bluuebunny/crossref_metadata_2025 huggingface dataset:
Dysbiosis of Fecal Microbiota From Complement 3 Knockout Mice With Constipation Phenotypes Contributes to Development of Defecation Delay
Authors: Choi Yun Ju , Kim Ji Eun , Lee Su Jin , Gong Jeong Eun , Son Hong Joo , Hong Jin Tae , Hwang Dae Youn
Published: July 2021

Significant phenotypes for constipation were detected in complement 3 (C3) knockout (KO) mice, although no research has been conducted on an association with alteration of gut microbiota. To investigate the effects of dysbiosis on fecal microbiota from C3 KO mice with constipation, the composition of fecal microbiota was characterized in mid-colons of 16-week-old C3 KO mice, and their function for defecation delay development was examined after fecal microbiota transplantation (FMT) of C3 KO mice. Some significant alterations in constipation phenotypes, including stool parameters and histopathological structure, were detected in 16-week-old C3 KO mice compared to those of wild-type (WT) mice. Fecal microbiota of C3 KO mice exhibited decreases inAnaerocolumna,Caecibacterium,Christensenella,Kineothrix, andOscillibacterpopulations and increases inPrevotellamassilia,Reuthenibacterium,Prevotella,Eubacterium,Culturomica,Bacteroides, andMuribaculumpopulations. In FMT study, key stool parameters, including weight and water content, were remarkably declined in a transplanted KO (KFMT) group of antibiotics-induced depletion of microbiota (AiDM)-WT and AiDM-KO mice, and a similar change was observed in fecal morphology. However, intestine length decreased in only the KFMT group of AiDM-WT mice compared with that of AiDM-KO mice. The mucosal layer and muscle thickness were commonly decreased in the KFMT group of AiDM-WT and AiDM-KO mice, and significant alterations in the crypt structure of Lieberkuhn and molecular regulators, including AQP8, C-kit, and 5-HT, were observed in the same group. Taken together, results of the present study indicate that dysbiosis of fecal microbiota from C3 KO mice with constipation phenotypes has a key role in the induction and regulation of defecation delay.
Exercise Intensity and Pacing Pattern During a Cross-Country Olympic Mountain Bike Race
Authors: Næss Steffan , Sollie Ove , Gløersen Øyvind Nøstdahl , Losnegard Thomas
Published: July 2021

Objective: To examine the power profiles and pacing patterns in relation to critical power (CP) and maximal aerobic power (MAP) output during a cross-country Olympic (XCO) mountain bike race.

Methods: Five male and two female national competitive XCO cyclists completed a UCI Cat. 1 XCO race. The races were 19 km and 23 km and contained five (female) and six (male) laps, respectively. Power output (PO) during the race was measured with the cyclists’ personal power meters. On two laboratory tests using their own bikes and power meters, CP and work capacity above CP (W') were calculated using three time trials of 12, 7, and 3 min, while MAP was established based on a 3-step submaximal test and the maximal oxygen uptake from the 7-min time trial.

Results: Mean PO over the race duration (96 ± 7 min) corresponded to 76 ± 9% of CP and 63 ± 4% of MAP. 40 ± 8% of race time was spent with PO > CP, and the mean duration and magnitude of the bouts >CP was ~8 s and ~120% of CP. From the first to last lap, time >CP and accumulated W' per lap decreased with 9 ± 6% and 45 ± 17%, respectively. For single >CP bouts, mean magnitude and mean W' expended decreased by 25 ± 8% and 38 ± 15% from the first to the last lap, respectively. Number and duration of bouts did not change significantly between laps.

Conclusion: The highly variable pacing pattern in XCO implies the need for rapid changes in metabolic power output, as a result of numerous separate short-lived >CP actions which decrease in magnitude in later laps, but with little lap-to-lap variation in number and duration.
Rewiring Meiosis for Crop Improvement
Authors: Kuo Pallas , Da Ines Olivier , Lambing Christophe
Published: July 2021

Meiosis is a specialized cell division that contributes to halve the genome content and reshuffle allelic combinations between generations in sexually reproducing eukaryotes. During meiosis, a large number of programmed DNA double-strand breaks (DSBs) are formed throughout the genome. Repair of meiotic DSBs facilitates the pairing of homologs and forms crossovers which are the reciprocal exchange of genetic information between chromosomes. Meiotic recombination also influences centromere organization and is essential for proper chromosome segregation. Accordingly, meiotic recombination drives genome evolution and is a powerful tool for breeders to create new varieties important to food security. Modifying meiotic recombination has the potential to accelerate plant breeding but it can also have detrimental effects on plant performance by breaking beneficial genetic linkages. Therefore, it is essential to gain a better understanding of these processes in order to develop novel strategies to facilitate plant breeding. Recent progress in targeted recombination technologies, chromosome engineering, and an increasing knowledge in the control of meiotic chromosome segregation has significantly increased our ability to manipulate meiosis. In this review, we summarize the latest findings and technologies on meiosis in plants. We also highlight recent attempts and future directions to manipulate crossover events and control the meiotic division process in a breeding perspective.
The Scaling and Modeling of Pay and the Robustness of the Effect of Core Self Evaluations on Career Success
Authors: Ganzach Yoav , Pazy Asya
Published: July 2021

A number of recent studies used nominal pay in estimating the effects of individual differences, particularly core-self-evaluation, on career success. We show that this practice may lead to results that are substantively different from the results when the logarithm of pay is used. We conduct three constructive replications of previous studies, and argue that substantive conclusion based on the results of nominal pay are misleading.
Identifying Interaction Patterns of Tangible Co-Adaptations in Human-Robot Team Behaviors
Authors: van Zoelen Emma M. , van den Bosch Karel , Rauterberg Matthias , Barakova Emilia , Neerincx Mark
Published: July 2021

As robots become more ubiquitous, they will increasingly need to behave as our team partners and smoothly adapt to the (adaptive) human team behaviors to establish successful patterns of collaboration over time. A substantial amount of adaptations present themselves through subtle and unconscious interactions, which are difficult to observe. Our research aims to bring about awareness of co-adaptation that enables team learning. This paper presents an experimental paradigm that uses a physical human-robot collaborative task environment to explore emergent human-robot co-adaptions and derive the interaction patterns (i.e., the targeted awareness of co-adaptation). The paradigm provides a tangible human-robot interaction (i.e., a leash) that facilitates the expression of unconscious adaptations, such as “leading” (e.g., pulling the leash) and “following” (e.g., letting go of the leash) in a search-and-navigation task. The task was executed by 18 participants, after which we systematically annotated videos of their behavior. We discovered that their interactions could be described by four types of adaptive interactions: stable situations, sudden adaptations, gradual adaptations and active negotiations. From these types of interactions we have created a language of interaction patterns that can be used to describe tacit co-adaptation in human-robot collaborative contexts. This language can be used to enable communication between collaborating humans and robots in future studies, to let them share what they learned and support them in becoming aware of their implicit adaptations.
Positive Psychological Capital as a Predictor of Satisfaction With the Fly-In Fly-Out Model
Authors: Soares Marques Nazaré , Lopes Miguel Pereira , Gonçalves Sónia P.
Published: July 2021

The flexibility of markets and international agreements have lured a growing number of companies to expand their business beyond frontiers in search for new markets and a bigger business network. Specifically, expatriates became keystones to implant and promote the so desired expansion into international markets, Particularly, Fly-in fly-out (FIFO) flexpatriates. Although FIFO work practices are widely used, little is known about how to promote these professionals’ perceived job satisfaction (JS) across the course of their work cycles. That is why the goal of our research is to test the positive psychological capital (PsyCap) applicability to Portuguese FIFO flexpatriates. In the midst of the positive psychology theories, Luthans et al. (2007b) underline that workers are the psychological capital of any organization. Therefore, the development of the PsyCap becomes crucial and also contributes to the promotion of JS, nowadays a construct intertwined with well-being. As such, we developed and applied a HERO–(hope, self-efficacy, resilience, and optimism)–micro-intervention in order to assess whether it moderated the relationship between a FIFO flexpatriates PsyCap and their JS. The research took place over three distinct moments, both PsyCap and JS were measured before and after the HERO micro-intervention, and again 3 months later. The data collected shows that a positive correlation exists between FIFO flexpatriates PsyCap and JS. Moreover, our results pointed out that the micro-intervention enhanced FIFO flexpatriates PsyCap, and also showed that this increase lasted over (at least) 3 months.
Unsolicited Pics and Sexual Scripts: Gender and Relationship Context of Compliant and Non-consensual Technology-Mediated Sexual Interactions
Authors: Courtice Erin Leigh , Czechowski Konrad , Noorishad Pari-Gole , Shaughnessy Krystelle
Published: July 2021

Technology-mediated sexual interaction (TMSI) refers to any partnered interaction that involves sending or receiving self-created, sexually explicit content using communication technology (e. g., sexting, cybersex). Most research on TMSI assumes that experiences are desired and consensual. However, it is likely that some people do not desire all their TMSI experiences but consent to them anyways (compliance), or experience non-consensual TMSIs. People also engage in TMSIs with different types of partners. According to the traditional sexual script (TSS), other-gender attracted women and men's non-consensual TMSI experiences should differ overall and depending on the relationship context of the experience. The goal of this study was to examine the role of sexual scripts in other-gender attracted women and men's non-consensual and compliant TMSI experiences with committed romantic partners (CRPs), known non-partners (KNPs), and strangers (Ss). Women (n = 331) and men (n = 120) completed an online survey with questions about lifetime prevalence of experiencing seven types of compliant and non-consensual TMSIs in each relationship context. Results of mixed ANOVAs revealed significant interactions: overall, more participants reported compliant TMSI with CRPs. More women than men had received a non-consensual TMSI from someone they were not in a committed relationship with, and more men than women reported sending non-consensual TMSIs to a stranger. Tests of unpaired proportions suggested that the prevalence of sending and receiving non-consensual TMSIs was discordant in the KNP and S contexts: both women and men received more non-consensual TMSIs from KNPs and Ss than the other-gender reported sending. Our findings suggest that gendered sexual scripts are evident in some, but not all, aspect of other-gender attracted women and men's compliant and non-consensual TMSI experiences.
Collaborating in Isolation: Assessing the Effects of the Covid-19 Pandemic on Patterns of Collaborative Behavior Among Working Musicians
Authors: Fram Noah R. , Goudarzi Visda , Terasawa Hiroko , Berger Jonathan
Published: July 2021

The Covid-19 pandemic severely limited collaboration among musicians in rehearsal and ensemble performance, and demanded radical shifts in collaborative practices. Understanding the nature of these changes in music creators' patterns of collaboration, as well as how musicians shifted prioritizations and adapted their use of the available technologies, can offer invaluable insights into the resilience and importance of different aspects of musical collaboration. In addition, assessing changes in the collaboration networks among music creators can improve the current understanding of genre and style formation and evolution. We used an internet survey distributed to music creators, including performers, composers, producers, and engineers, all active before and during the pandemic, to assess their perceptions of how their music, collaborative practice, and use of technology were impacted by shelter-in-place orders associated with Covid-19, as well as how they adapted over the course of the pandemic. This survey was followed by Zoom interviews with a subset of participants. Along with confirming previous results showing increased reliance on nostalgia for musical inspiration, we found that participants' collaborative behaviors were surprisingly resilient to pandemic-related changes. In addition, participant responses appeared to be driven by a relatively small number of underlying factors, representing approaches to musical collaboration such as musical extroversion or musical introversion, inspiration clusters such as activist musicking, and style or genre clusters.
Scale Characteristics of Intercultural Competence Measures and the Effects of Intercultural Competence on Prejudice
Authors: Genkova Petia , Schaefer Christoph Daniel , Schreiber Henrik , Rašticová Martina , Poor Jozsef , Veresné Klara Valentinyi , Suhajda Csilla , Viszetenvelt Andrea , Bjekic Jovana
Published: July 2021

Due to proceeding globalization processes, involving a rise in mobility and international interdependencies, the frequency and relevance of intercultural contact situations increases. Consequently, the ability to deal effectively with intercultural situations is gaining in importance. However, the majority of studies on measures of intercultural competence focuses on Western Europe and the United States or cultures of the Far East. For the present study, previously understudied Eastern European (former communist) cultures were included, by sampling in Hungary, Serbia, and the Czech Republic, in addition to (the Central or Western European country) Germany. Thus, this study enabled comparisons of scale characteristics of the cultural intelligence scale (CQS), the multicultural personality questionnaire (MPQ), as well as the blatant and subtle prejudice scales, across samples from different cultures. It was also examined how the CQS and MPQ dimensions are associated with prejudice. To analyse scale characteristics, the factor structures and measurement invariances of the used instruments were analyzed. There were violations of configural measurement invariance observed for all of these scales, indicating that the comparability across samples is limited. Therefore, each of the samples was analyzed separately when examining how the CQS and MPQ dimensions are related to prejudice. It was revealed that, in particular, the motivational aspect of the CQS was statistically predicting lower prejudice. Less consistently, the MPQ dimensions of open-mindedness and flexibility were statistically predicting lower prejudice in some of the analyses. However, the violations of measurement invariance indicate differences in the constructs' meanings across the samples from different cultures. It is consequently argued that cross-cultural equivalence should not be taken for granted when comparing Eastern and Western European cultures.
How Neurobiology Elucidates the Role of Emotions in Financial Decision-Making
Authors: Bossaerts Peter
Published: July 2021

Over the last 15 years, a revolution has been taking place in neuroscience, whereby models and methods of economics have led to deeper insights into the neurobiological foundations of human decision-making. These have revealed a number of widespread mis-conceptions, among others, about the role of emotions. Furthermore, the findings suggest that a purely behavior-based approach to studying decisions may miss crucial features of human choice long appreciated in biology, such as Pavlovian approach. The findings could help economists formalize elusive concepts such as intuition, as I show here for financial “trading intuition.”
Toward the Role of Language Teacher Confirmation and Stroke in EFL/ESL Students’ Motivation and Academic Engagement: A Theoretical Review
Authors: Gao Yun
Published: July 2021

Following the recent special issue in the journal of Frontiers in Psychology, named “The Role of Teacher Interpersonal Variables in Students’ Academic Engagement, Success, and Motivation,” this review is carried out to describe two prime instances of teacher interpersonal behaviors, namely teacher confirmation and stroke, their underlying frameworks, and contributions to desirable student-related outcomes. In light of rhetorical-relational goal theory and the school of positive psychology, it is stipulated that language teacher confirmation and stroke are facilitators of EFL/ESL students’ level of motivation and academic engagement. Providing empirical evidence, the argument regarding the pivotal role of language teacher confirmation and stroke in EFL/ESL contexts was proved. Reviewing the available literature on the aforementioned variables, some pedagogical implications were suggested for teacher trainers, educational supervisors, and pre- and in-service language teachers. Finally, the limitations and drawbacks of the reviewed studies were identified and some avenues for further research were recommended, accordingly.
Does Psychedelic Therapy Have a Transdiagnostic Action and Prophylactic Potential?
Authors: Kočárová Rita , Horáček Jiří , Carhart-Harris Robin
Published: July 2021

Addressing global mental health is a major 21st-century challenge. Current treatments have recognized limitations; in this context, new ones that are prophylactic and effective across diagnostic boundaries would represent a major advance. The view that there exists a core of transdiagnostic overlap between psychiatric disorders has re-emerged in recent years, and evidence that psychedelic therapy holds promise for a range of psychiatric disorders supports the position that it may be transdiagnostically effective. Here, we propose that psychedelic therapy's core, transdiagnostically relevant action lies in its ability to increase neuronal and mental plasticity, thus enhancing the potential for change, which we consider to be a key to its therapeutic benefits. Moreover, we suggest that enhanced plasticityviapsychedelics, combined with a psychotherapeutic approach, can aid healthy adaptability and resilience, which are protective factors for long-term well-being. We present candidate neurological and psychological markers of this plasticity and link them with a predictive processing model of the action of psychedelics. We propose that a model of psychedelic-induced plasticity combined with an adequate therapeutic context has prophylactic and transdiagnostic potential, implying that it could have a broad, positive impact on public health.
The Introduction of Medication-Free Mental Health Services in Norway: An Analysis of the Framing and Impact of Arguments From Different Standpoints
Authors: Nyttingnes Olav , Rugkåsa Jorun
Published: July 2021

Introduction: Debates about coercive practices have challenged a traditional biomedical hegemony in mental health care. The perspectives of service user organizations have gained considerable ground, such as in the development of the Convention on the Rights of Persons with Disabilities. Such changes are often contested, and might in practice be a result of (implicit) negotiation between stakeholders with different discursive positions. To improve understanding of such processes, and how discursive positions may manifest and interact, we analyzed texts published over a 10 year period related to the introduction of medication-free inpatient services in Norway.

Methods: We conducted qualitative analyses of 36 policy documents related to the introduction of medication-free services and 75 opinion pieces from a subsequent debate. We examined discursive practices in these texts as expressions of what is perceived as legitimate knowledge upon which to base mental health care from the standpoints of government, user organizations and representatives of the psychiatric profession. We paid particular attention to how standpoints were framed in different discourse surrounding mental health care, and how these interacted and changed during the study period (2008–2018).

Results: The analysis shows how elements from the discourse promoted by service user organizations—most notably the legitimacy of personal experiences as a legitimate source of knowledge—entered the mainstream by being incorporated into public policy. Strong reactions to this shift, firmly based in biomedical discourse, endorsed evidence-based medicine as the authoritative source of knowledge to ensure quality care, although accepting patient involvement. Involuntary medication, and how best to help those with non-response to antipsychotic medication represented a point at which discursive positions seemed irreconcilable.

Conclusion: The relative authorities of different sources of knowledge remain an area of contention, and especially in determining how best to help patients who do not benefit from antipsychotics. Future non-inferiority trials of medication-free services may go some way to break this discursive deadlock.
Cognitive Dysfunction in Insomnia Phenotypes: Further Evidence for Different Disorders
Authors: Olaithe Michelle , Ree Melissa , McArdle Nigel , Donaldson Sara , Pushpanathan Maria , Eastwood Peter R. , Bucks Romola S.
Published: July 2021

Study Objectives: To determine cognitive profiles in individuals with short sleep duration insomnia (SSDI) and normal sleep duration insomnia (NSDI; also, paradoxical insomnia), compared to healthy sleepers.

Method: Polysomnographic (PSG) and neuropsychological data were analysed from 902 community-based Raine Study participants aged 22 ± 0.6 years of whom 124 met criteria for insomnia (53 with NSDI and 71 with or SSDI) and 246 were classified as healthy with normal sleep (i.e., without insomnia or other sleep disorders). Measurements of self- report (attention and memory) and laboratory-assessed (attention, episodic memory, working memory, learning, and psychomotor function) cognition and mood, and PSG-based sleep stages (% total sleep time; %TST) were compared between these 3 groups.

Results: In comparison to the healthy sleeper group, both insomnia groups had poorer self-reported attention, memory, mood, and sleep, and poorer laboratory-assessed attention (inconsistency). The NSDI group had less consistent working memory reaction time than healthy-sleepers or those with SSDI. The SSDI group had more inconsistency in executive function (shifting), and showed greater %TST in stage N1 and N3, and less REM sleep than either healthy-sleepers or those with NSDI.

Conclusions: Individuals with NSDI demonstrated greater working memory inconsistency, despite no laboratory assessed sleep problems, implicating early signs of pathophysiology other than disturbed sleep. Those with SSDI demonstrated different sleep architecture, poorer attention (inconsistency), and greater executive function (inconsistency) compared to healthy-sleepers and those with NSDI, implicating sleep disturbance in the disease process of this phenotype.
Genetic Contribution of Synapse-Associated Protein 97 to Orbitofrontal–Striatal–Thalamic Circuitry Connectivity Changes in First-Episode Schizophrenia
Authors: Xu Xusan , Luo Shucun , Wen Xia , Wang Xiaoxia , Yin Jingwen , Luo Xudong , He Bin , Liang Chunmei , Xiong Susu , Zhu Dongjian , Fu Jiawu , Lv Dong , Dai Zhun , Lin Juda , Li You , Lin Zhixiong , Chen Wubiao , Luo Zebin , Wang Yajun , Ma Guoda
Published: July 2021

Functional and structural disturbances in the orbitofrontal–striatal–thalamic circuitry are thought to be associated with mental symptoms and neurocognitive impairments in schizophrenia. This study tested whether synapse-associated protein 97 (SAP97), a reasonable candidate gene for schizophrenia, is related to orbitofrontal–striatal–thalamic connection changes in first-episode schizophrenia (FES) patients and the clinical performance of schizophrenic patients by affecting this integrity. Fifty-two FES patients and 52 matched healthy controls were recruited. All subjects underwent genotyping via the improved multiplex ligation detection reaction technique and scanning with magnetic resonance imaging (MRI) to provide orbitofrontal–striatal–thalamic functional and structural imaging data. A two-way analysis of covariance model was employed to examine abnormal brain connectivities, and Spearman correlations were applied to estimate the relationships between brain connectivity and clinical manifestations. In the FES group, those with the SAP97 rs3915512 TT genotype showed lower structural and functional connectivity than A allele carriers between the orbitofrontal gyrus and striatum/thalamus. In the FES group, negative correlations were found between resting-state functional connectivity (RSFC) in the orbitofrontal gyrus and thalamus, and positive symptoms between structural connections in the orbitofrontal gyrus and striatum and cognitive functions, and positive correlations were suggested between RSFC in the orbitofrontal gyrus and thalamus and negative symptoms. Our findings suggested that the SAP97 rs3915512 polymorphism may be involved in mental symptoms and cognitive dysfunction in FES patients by influencing structural and functional connectivity of the orbitofrontal–striatal and orbitofrontal–thalamic regions.
Long-Term (2003–2019) Air Quality, Climate Variables, and Human Health Consequences in Dhaka, Bangladesh
Authors: Pavel Md Riad Sarkar , Zaman Shahid Uz , Jeba Farah , Islam Md Safiqul , Salam Abdus
Published: July 2021

Long-term trends in air quality by studying the criteria pollutants (PM2.5, PM10, CO, O3, NO2, and SO2) and climate variables (temperature, surface pressure, and relative humidity) were depicted in this study. The 17-year (2003–2019) average values of PM2.5, PM10, CO, O3, NO2, and SO2 were 88.69 ± 9.76 μg/m3, 124.57 ± 12.75 μg/m3, 0.69 ± 0.06 ppm, 51.42 ± 1.82 ppb, 14.87 ± 2.45 ppb, and 8.76 ± 2.07 ppb, respectively. The trends among the ambient pollutants were increasingly significant (p < 0.05) except for O3 with slopes of 1.83 ± 0.15 μg/m3/year, 2.35 ± 0.24 μg/m3/year, 0.01 ± 0.002 ppm/year, 0.47 ± 0.03 ppb/year, and 0.40 ± 0.02 ppb/year for PM2.5, PM10, CO, NO2, and SO2, respectively. Pearson correlations revealed a significant association among the pollutants while a noteworthy correlation was observed between ambient pollutants and surface temperature. Principal component analysis (PCA) and positive matrix factorization (PMF) have been employed collectively to examine the main sources of the pollutants. PCA revealed similar trends for PMs and CO, as well as NO2 and SO2 being equally distributed variables. PMF receptor modeling resulted in attributing four sources to the pollutants. The factors inferred from the PMF modeling were signified as vehicular emissions, road/soil dust, biomass burning, and industrial emissions. The hazard quotient (HQ) values were not antagonistic (HQ < 1) in acute exposure levels for the three age groups (infants, children, and adults) while showing significant health risk (HQ > 1) in chronic exposure for infants and children. Children are identified as the worst sufferers among the age groups, which points to low breathing levels and high exposure to traffic pollution in Dhaka, Bangladesh.
Characteristics and Dynamics of the Freshwater Fish Market in Chengdu, China
Authors: Fang Julian , Fabinyi Michael
Published: July 2021

The drivers and characteristics of trends in aquatic product consumption are a crucial component of fish food system sustainability. The Chinese market for aquatic products is the largest in the world, yet little has been published on the characteristics of the freshwater fish market. This Paper draws on interviews with key informants to understand the social characteristics of the freshwater fish market in Chengdu, Sichuan province. Price, food safety and quality, freshness and local culinary traditions are important influences on patterns of freshwater fish consumption. However, imported species such as pangasius and branded products are increasing in popularity, indicative of changes in the Chengdu freshwater fish market and the Chinese market for aquatic products more generally.
Post-harvest Management of Alternaria Induced Rot in Tomato Fruits With Essential Oil of Zanthoxylum armatum DC
Authors: Slathia Shummu , Sharma Yash Pal , Hakla Haroon Rashid , Urfan Mohammad , Yadav Narendra Singh , Pal Sikander
Published: July 2021

Alternaria fruit rot is a major disease caused byAlternaria alternata(Fr.) Keissl., a prolific fungal pathogen. Among post-harvest diseases of tomato, fruit rot induced byA.alternatais the most damaging. Antifungal agents are widely used to control post-harvest management of tomato fruits. However, negative impacts of fungicidal residues in edible fruits and vegetables on human health cannot be over ruled. Eco-friendly ways of controlling Alternaria rot in tomato fruits offer a novel way of tomato rot management. The current study proposes an alternate method in controlling tomato fruit rots throughZanthoxylum armatumDC essential oil (EO) application. Gas chromatography-mass spectrometry profiling showed eucalyptol and sabinene as major components ofZ.armatumEO. Furthermore, EO applied (0.5–4.5 μl/ml) showed significant inhibition ofA. alternatagrowth (p> 0.05) at 4.5 μl concentration tested. Lipid peroxidation assays revealed significant reduction in membrane damage in tomato fruits treated by EO compared to alone inoculated fruits withA.alternata. Elevated activities of superoxide dismutase, catalase, ascorbate peroxidase, and glutathione reductase coupled with enhanced antioxidants such as ascorbic acid, glutathione, proline, and total phenols in EO-treated fruits may be linked with better fruit rot management than control fruits inoculated withA.alternata-induced rot alone. Mycelia and spore production was dramatically reduced in EO applied tomato fruits overA.alternataalone in tomato fruits (p> 0.05). Interestingly, free radical scavenging activities of EO applied tomato fruits showed significant improvement compared to only pathogen-inoculated tomato fruits. Findings propose practical utility ofZ.armatumEO as a plant-based antifungal for post-harvest management of Alternaria rot in tomato fruits.
Progress on Genetic Improvement and Analysis of Bottle Gourd [Lagenaria siceraria (Molina) Standl.] for Agronomic Traits, Nutrient Compositions, and Stress Tolerance: A Review
Authors: Mkhize Phumzile , Mashilo Jacob , Shimelis Hussein
Published: July 2021

Bottle gourd [Lagenaria siceraria(Molina) Standl.] is an important multi-purpose cucurbit crop grown for its leaf, fruit, and seed. It is widely cultivated and used for human consumption in sub-Saharan Africa (SSA) providing vital human nutrition and serving as food security crop. There is wide genetic variation among bottle gourd genetic resources in Africa for diverse qualitative and quantitative attributes for effective variety design, product development, and marketing. However, the crop is under- researched and -utilized, and improved varieties are yet to be developed and commercialized in the region. Therefore, the objective of this review is to provide the progress on bottle gourd genetic improvement and genetic analysis targeting agronomic and horticultural attributes, nutritional composition, biotic, and abiotic stress tolerance to guide current and future cultivar development, germplasm access, and conservation in SSA. The first section of the paper presents progress on breeding of bottle gourd for horticultural traits, agronomic performance, nutritional and anti-nutritional composition, and biotic and abiotic stress tolerance. This is followed by important highlights on key genetic resources of cultivated and wild bottle gourd for demand driven breeding. Lastly, the review summaries advances in bottle gourd genomics, genetic engineering and genome editing. Information presented in this paper should aid bottle gourd breeders and agronomists to develop and deploy new generation and promising varieties with farmer- and market -preferred attributes.
Effect of Weeping Teats on Intramammary Infection and Somatic Cell Score in Dairy Goats
Authors: Gazzola Alessandra , Minozzi Giulietta , Biffani Stefano , Mattiello Silvana , Bailo Giovanni , Piccinini Renata
Published: July 2021

Mastitis is the most common disease affecting dairy goats and causing economic losses. Although it is accepted that increased somatic cell count (SCC) is mainly a response to infection, its reliability for subclinical mastitis detection in goats is controversial. Indeed, many physiological and extrinsic variables can increase SCC, including breed, parity, age, stage of lactation, seasonal variations, and milking methods. In some animals, milk-secreting tissue is present in the wall of the teat and, in some instances, milk can filter through pores in the skin to the udder surface. This condition is known as “weeping teat” (WT). In these animals, mammary tissue might be prone to develop bacterial infections, although limited information is provided. Weeping teat seems to have a genetic background and is reported to be especially found in goat breeds selected for high milk production. Moreover, it is observed a genetic correlation between WT and decreased milk yield as well as increased somatic cell scores (SCS). Since information on this topic is very limited, this study aimed at investigating any possible relationship between WT, high SCC, and the presence of bacteria in goat milk. Alpine goat farms in Northern Italy were selected based on the presence of WT. Each herd was divided into two age-matched groups, identified as case (WT+) and control (WT–). Half-udder milk samples were collected aseptically at three timepoints; bacteriological analysis was performed, and SCC were determined and transformed in SCS. There was a positive association between SCS and the presence of bacteria in milk (P = 0.037) overall, whereas WT udder defect was associated with positive bacterial culture in just one herd (P = 0.053). Thus, this herd was further investigated, repeating the sampling and the analysis on the following year. The positive association between high SCS and the presence of bacteria in milk was then confirmed (P = 0.007), whereas no association with WT condition was found. These results indicate that WT defect is usually unrelated to both the outcome of milk bacterial culture and SCS. As a side outcome, we could confirm the role of bacterial infection in increasing SCS.
The Principles of the Voluntary Programme for the Control and Elimination of Bovine Viral Diarrhoea Virus (BVDV) From Infected Herds in Slovenia
Authors: Toplak Ivan , Hostnik Peter , Černe Danijela , Mrkun Janko , Starič Jože
Published: July 2021

In Slovenia, the control of bovine viral diarrhoea virus (BVDV) infections started in 1994. Since 2014, a voluntary programme has been running according to the national rules that prescribe the conditions for recognising, acquiring, and maintaining a BVDV-free status for an individual herd. The principle is based on periodical laboratory testing and preventive measures that need to be strictly implemented in a herd. Between 2014 and 2020, a total of 348 herds were included in BVDV antibody testing, and 25.0% of tested herds were detected to be BVDV antibody positive. To recognise the BVDV-free status of the herd, the breeder should provide two consecutive tests with intervals of at least 6 months in all animals in the age from 7 to 13 months, with negative results for BVDV antibodies in ELISA. The BVDV-free status of the herd can be maintained by implementing preventive measures and can be renewed each year with one laboratory test in the age group of animals from 7 to 13 months for antibodies in ELISA. During the 7 years of the voluntary programme, 236 herds were included in the detection of BVDV in individual herds by real-time RT-PCR method and the elimination of positive animals from herds. In 71 (31.3%) herds, at least one BVDV-positive animal was detected, with the identification of a total of 267 persistently infected (PI) animals, representing an average of 2.9% of tested animals. The cost of testing for an average herd, recognised as BVDV-negative, and maintaining its BVDV-free status within the implemented voluntary programme, was €97.64/year, while for the average positive herd, the laboratory costs for elimination of BVDV were €189.59/year. Only limited progress towards eradication at the national level has been achieved in Slovenia since 2014.
Siblings as a Context for Positive Development: Closeness, Communication, and Well-Being
Authors: LeBouef Samantha , Dworkin Jodi
Published: July 2021

To address a gap in the literature we examined sibling relationships as a context for positive development. Specifically, the relationships between individual well-being, sibling closeness, and frequency of sibling communication were explored. The goal of this study was to determine whether individual well-being moderated the relationship between frequency of sibling communication and perceived sibling closeness. Participants included a subsample (n = 236) of youth from a larger study who reported having at least one sibling. Data were collected through Amazon’s Mechanical Turk. Hierarchical multiple regression analyses revealed that associations between frequency of sibling communication and sibling closeness were statistically significant, sibling communication and closeness were highly correlated, regardless of individual differences. Neither emotional, psychological, nor social well-being moderated the relationship between frequency of sibling communication and sibling closeness. Limitations and future directions are considered.
PSO-Based Soft Lunar Landing with Hazard Avoidance: Analysis and Experimentation
Authors: D’Ambrosio Andrea , Carbone Andrea , Spiller Dario , Curti Fabio
Published: July 2021

The problem of real-time optimal guidance is extremely important for successful autonomous missions. In this paper, the last phases of autonomous lunar landing trajectories are addressed. The proposed guidance is based on the Particle Swarm Optimization, and the differential flatness approach, which is a subclass of the inverse dynamics technique. The trajectory is approximated by polynomials and the control policy is obtained in an analytical closed form solution, where boundary and dynamical constraints are a priori satisfied. Although this procedure leads to sub-optimal solutions, it results in beng fast and thus potentially suitable to be used for real-time purposes. Moreover, the presence of craters on the lunar terrain is considered; therefore, hazard detection and avoidance are also carried out. The proposed guidance is tested by Monte Carlo simulations to evaluate its performances and a robust procedure, made up of safe additional maneuvers, is introduced to counteract optimization failures and achieve soft landing. Finally, the whole procedure is tested through an experimental facility, consisting of a robotic manipulator, equipped with a camera, and a simulated lunar terrain. The results show the efficiency and reliability of the proposed guidance and its possible use for real-time sub-optimal trajectory generation within laboratory applications.
Spatial Distribution and Development of Sequential Sampling Plans for Diaphorina citri Kuwayama (Hemiptera: Liviidae)
Authors: Díaz-Padilla Gabriel , López-Arroyo J. Isabel , Guajardo-Panes Rafael A. , Sánchez-Cohen Ignacio
Published: July 2021

Vector control in huanglongbing management has been conducted on a calendar basis resulting in high production costs. We addressed this issue and proposed a sequential sampling plan to support decision making for intervention against Diaphorina citri Kuwayama, which is involved in the transmission of the bacteria Candidatus Liberibacter asiaticus, associated with such lethal disease. We analyzed 3,264,660 records from samples gathered from the Mexican trapping program for the monitoring of D. citri; it included weekly inspection of 86,004 yellow sticky traps distributed in the country. Spatial distribution of the insect, estimation of a common k (kc), and sequential sampling plans based on Sequential Probability Ratio Test (SPRT) were determined. Taylor’s power law coefficients were ≥1 indicating aggregation in the spatial distribution of the insect. Common k ranged from 0.0183 to 0.2253 and varied independently of geographic zone or citrus species. We obtained 18 sequential sampling plans, one for each state. In the Average Sample Number (ASN) function, the minimal number of samples to make a decision ranged from 17 to 65. In the Operational Characteristic (OC) function, probabilities for a correct intervention at the threshold of 0.2 D. citri adults/trap in most cases were above 80%. In a field evaluation, the application of sampling plans yielded savings obtained by reduction in the number of interventions for insect control.
Porcine Protein Hydrolysates (PEPTEIVA®) Promote Growth and Enhance Systemic Immunity in Gilthead Sea Bream (Sparus aurata)
Authors: Gisbert Enric , Ibarz Antoni , Firmino Joana P. , Fernández-Alacid Laura , Salomón Ricardo , Vallejos-Vidal Eva , Ruiz Alberto , Polo Javier , Sanahuja Ignasi , Reyes-López Felipe E. , Tort Lluis , Andree Karl B.
Published: July 2021

The effects of porcine plasma protein hydrolysate (PPH) on growth, feed efficiency, and immune responses was evaluated in Sparus aurata. Fish were fed two isoproteic (48% protein), isolipidic (17% fat), and isoenergetic diets (21.7 MJ/kg) diets, one of them containing 5% PPH at the expense of fishmeal. Both diets were tested for 92 days. A significant increase in growth was observed in fish fed the PPH diet in comparison to the control group (182.2 ± 4.4 vs. 173.8 ± 4.1 g), as well as an increase in feed intake without worsening FCR values. An ex vivo assay, with splenocytes incubated with lipopolysaccharide, was conducted to evaluate the cellular immune competence of fish. Genes involved in humoral immunity (lys, IgM), pro- (tnf-α, il-1β), and anti-inflammatory (tgf-β1, il10) cytokines were upregulated in the PPH group in comparison to the control group. The inclusion of PPH in diets enhanced the antibacterial capacity of skin mucus, as the co-culture of selected bacteria (E. coli, V. anguillarum, and P. anguilliseptica) with skin mucus indicated. The present results showed that the PPH in low fishmeal diets (2%) promoted growth and feed efficiency, as well as enhancing the immune response, which indicates that this is a safe and functional ingredient for aquafeeds.
Effects of Dietary Supplementation with Protected Sodium Butyrate on Gut Microbiota in Growing-Finishing Pigs
Authors: Bernad-Roche María , Bellés Andrea , Grasa Laura , Casanova-Higes Alejandro , Mainar-Jaime Raúl Carlos
Published: July 2021

The study assessed changes in the gut microbiota of pigs after dietary supplementation with protected sodium butyrate (PSB) during the growing-fattening period (≈90 days). One gram of colon content from 18 pigs (9 from the treatment group -TG- and 9 from the control group -CG-) was collected. Bacterial DNA was extracted and 16S rRNA high-throughput amplicon sequencing used to assess microbiota changes between groups. The groups shared 75.4% of the 4697 operational taxonomic units identified. No differences in alpha diversity were found, but significant differences for some specific taxa were detected between groups. The low-represented phylum Deinococcus-Thermus, which is associated with the production of carotenoids with antioxidant, anti-apoptotic, and anti-inflammatory properties, was increased in the TG (p = 0.032). Prevotellaceae, Lachnospiraceae, Peptostreptococcaceae, Peptococcaceae, and Terrisporobacter were increased in the TG. Members of these families have the ability to ferment complex dietary polysaccharides and produce larger amounts of short chain fatty acids. Regarding species, only Clostridium butyricum was increased in the TG (p = 0.048). Clostridium butyricum is well-known as probiotic in humans, but it has also been associated with overall positive gut effects (increased villus height, improved body weight, reduction of diarrhea, etc.) in weanling pigs. Although the use of PSB did not modify the overall richness of microbiota composition of these slaughter pigs, it may have increased specific taxa associated with better gut health parameters.
Biocatalyzed Flow Oxidation of Tyrosol to Hydroxytyrosol and Efficient Production of Their Acetate Esters
Authors: Annunziata Francesca , Contente Martina L. , Pinna Cecilia , Tamborini Lucia , Pinto Andrea
Published: July 2021

Tyrosol (Ty) and hydroxytyrosol (HTy) are valuable dietary phenolic compounds present in olive oil and wine, widely used for food, nutraceutical and cosmetic applications. Ty and HTy are endowed with a number of health-related biological activities, including antioxidant, antimicrobial and anti-inflammatory properties. In this work, we developed a sustainable, biocatalyzed flow protocol for the chemo- and regio-selective oxidation of Ty into HTy catalyzed by free tyrosinase from Agaricus bisporus in a gas/liquid biphasic system. The aqueous flow stream was then in-line extracted to recirculate the water medium containing the biocatalyst and the excess ascorbic acid, thus improving the cost-efficiency of the process and creating a self-sufficient closed-loop system. The organic layer was purified in-line through a catch-and-release procedure using supported boronic acid that was able to trap HTy and leave the unreacted Ty in solution. Moreover, the acetate derivatives (TyAc and HTyAc) were produced by exploiting a bioreactor packed with an immobilized acyltransferase from Mycobacterium smegmatis (MsAcT), able to selectively act on the primary alcohol. Under optimized conditions, high-value HTy was obtained in 75% yield, whereas TyAc and HTyAc were isolated in yields of up to 80% in only 10 min of residence time.
On Combining Feature Selection and Over-Sampling Techniques for Breast Cancer Prediction
Authors: Huang Min-Wei , Chiu Chien-Hung , Tsai Chih-Fong , Lin Wei-Chao
Published: July 2021

Breast cancer prediction datasets are usually class imbalanced, where the number of data samples in the malignant and benign patient classes are significantly different. Over-sampling techniques can be used to re-balance the datasets to construct more effective prediction models. Moreover, some related studies have considered feature selection to remove irrelevant features from the datasets for further performance improvement. However, since the order of combining feature selection and over-sampling can result in different training sets to construct the prediction model, it is unknown which order performs better. In this paper, the information gain (IG) and genetic algorithm (GA) feature selection methods and the synthetic minority over-sampling technique (SMOTE) are used for different combinations. The experimental results based on two breast cancer datasets show that the combination of feature selection and over-sampling outperform the single usage of either feature selection and over-sampling for the highly class imbalanced datasets. In particular, performing IG first and SMOTE second is the better choice. For other datasets with a small class imbalance ratio and a smaller number of features, performing SMOTE is enough to construct an effective prediction model.