bluuebunny/crossref_metadata_2025 huggingface dataset:
Atuação dos profissionais da atenção primária na saúde mental materna
Authors: Rocha Flávia Ribeiro , Fernandes Bruna Bosi de Oliveira , Andrade Yan Victor Santana de , Arrais Alessandra da Rocha , Barros Ângela Ferreira
Published: November 2024

Objetivo: analisar a atuação dos profissionais da atenção primária ante as situações de sofrimento psíquico e/ou vulnerabilidade psicossocial de gestantes e puérperas. Métodos: estudo descritivo e exploratório, realizado com 78 profissionais de saúde atuantes na estratégia de saúde da família, aos quais foi aplicado um questionário de caracterização profissional e análise situacional. Resultados: a maioria dos profissionais de saúde atende diariamente gestantes e/ou puérperas (55,1%). Para 66,7% desses profissionais, na minoria dos atendimentos a esse público, eles identificam sinais e/ou sintomas de sofrimento emocional/psíquico/mental. Quanto à capacitação dos profissionais para atuar e ajudar gestantes e/ou puérperas em relação à saúde mental, 76,9% têm dúvidas e 89,7% não participaram de uma capacitação sobre o assunto e todos concordaram com sua relevância. Da análise textual das respostas discursivas resultaram duas categorias: Escuta qualificada como instrumento de acolhimento e Atendimento compartilhado e encaminhamento para profissional especializado. Conclusão: os profissionais da Estratégia Saúde da Família relataram ter dúvidas e fragilidades para o manejo da saúde mental de gestantes e puérperas. Contribuições para a prática: esses profissionais vivenciam situações que exigem deles o manejo de questões relacionadas à saúde mental perinatal e percebem como relevante uma capacitação para atenderem essa demanda.
Post-Discharge Cardiovascular Complications in Noncardiac Surgery: Incidence and Prediction
Authors: Sokolov D. A. , Kozlov I. A.
Published: November 2024

The aim of this study was to assess the incidence of cardiovascular complications (CVC) within 12 months after vascular surgery and to analyze inpatient perioperative examination data to identify potential predictors.

   Materials and Methods. A prospective cohort study included 103 patients aged 66 years [61–70] who underwent vascular surgery. Clinical outcomes within 12 months after surgery, including CVC and/or other cardiac events (composite outcome) and cardiac death, were assessed by telephone interviews with patients or their relatives. Patient physiological parameters, comorbidities, cardiac risk indices (CRI), platelet-lymphocyte ratio (PLR), concentration of N-terminal pro-B-type natriuretic peptide (NT-proBNP), and other parameters were obtained and analyzed from medical records. Logistic regression and ROC analysis were used to assess the predictive power of the investigated indicators.

   Results. The composite outcome was recorded in 33 % of cases and cardiac death occurred in 6.8 %. The risk of the composite outcome was associated with ASA class (OR 2.7413; 95 % CI 1.1126–6.7541), whereas the risk of perioperative myocardial infarction or cardiac arrest was associated with CRI (OR 1. 6051; 95 % CI 0.6645–2.0215), American University of Beirut (AUB) CRI (OR 2.1106; 95 % CI 1.0260–4.3414), PLR (1.0120; 95 % CI 1.0018–1.0222), and NT-proBNP concentration during hospitalization. Concurrent congestive heart failure (OR 5.0658; 95 % CI 1.2400–20.6956), revised CRI (OR 2.1024; 95 % CI 1.0572–4.1813), Khoronenko CRI (OR 103.76; 95 % CI 1.8752–5796.55), AUB CRI (OR 3.1902; 95 % CI 1.1040–9.2181), and NT-proBNP concentration all increased the risk of cardiac death. Predischarge NT-proBNP levels < 179 pg/mL (OR 1.0071; 95 % CI 1.0038–1.0104; AUC 0.795) and maximum postoperative NT-proBNP levels were reliable predictors of the composite outcome. The most effective predictor of postoperative mortality was a maximum NT-proBNP concentration > 303 pg/mL after surgery (OR 1.0039; 95 % CI 1.0015–1.0063; AUC 0.836).

   Conclusion. CVC developed in 33 % of patients within 12 months after vascular surgery, with cardiac death occurring in 6.8 % of cases. An NT-proBNP concentration > 179 pg/mL before hospital discharge or a maximum NT-proBNP concentration > 248 pg/mL in the postoperative period predicted CVC within one year. Postoperative NT-proBNP concentration > 303 pg/mL was a strong predictor of one-year cardiac mortality. Other factors associated with the risk of postoperative CVC did not provide an accurate prognosis.
Links Between Instagram Use and Fear of Missing Out Among Young Adults in Lithuania
Authors: Legkauskas Visvaldas , Žilinskaitė Ieva
Published: November 2024

The study involved 247 young adults (155 women and 92 men). The age of the subjects ranged from 18 to 34 years (mean age 22.8 years). 137 respondents indicated that they have secondary education, 86 respondents indicated that they have higher education, 16 respondents higher education and 8 basic education.
The study was conducted in the format of an online survey. Two quantitative research tools were used in the quantitative study – The Multidimensional Facebook Intensity Scale (Orosz, Toth-Kiraly, Bothe, 2016), which was adapted to investigate the intensity of Instagram use (Keyte et al., 2020), and the “Fear of Missing Out scale” (Przybylski et al., 2013). The subjects were asked two additional questions assessing the peculiarities of involvement in the Instagram network and socio-demographic questions.
The results of the study revealed that women are more likely to use the Instagram network, spend more time in the app, and have a higher expression of FOMO than men. The results of this study also showed that a stronger FOMO was associated with a longer time spent on Instagram among men. Among other things, the more pronounced FOMO is associated with more frequent checks of the Instagram app. Finally, the higher intensity of Instagram use by young adults is associated with a stronger expression of FOMO.
ELASTIC WAVES IN BODIES WITHIN WITH INITIAL (RESIDUAL) STRESSESSTRESSES
Authors: Guz A.N.
Published: November 2024

The monograph deals with a presentation of the theory of the elastic waves’ propagation in compressible and incompressible bodies with initial (residual) stresses. The results are based on using the three-dimensional linearized theory of elasticity for finite and small initial deformations. The theory and practical non-destructive determination of uniaxial, biaxial, and triaxial stresses in materials are scoped. The work is based on the results obtained at the Department of Dynamics and Stability continuum of the S.P. Timoshenko Institute of Mechanics of the NAS of Ukraine. The book is directed to specialists dealing with this theory and for the lecturers, postgraduate students, and students of corresponding specialties.
Restitution as a Prerequisite for Case Termination: Analyzing Conditional Exoneration in Russian Criminal Procedure
Authors: Grinenko Aleksandr , Ivanov Dmitriy , Kleshchina Elena , Alyshkin Vladislav , Bezryadin Viktor
Published: November 2024

In this scholarly article, the researchers methodically examine the protocols involved in discontinuing criminal cases through the conciliation of the involved parties, as well as ceasing criminal prosecution on the grounds of active contrition, contingent upon the pivotal requirement of recompensing the damage inflicted by the criminal act. The study substantiates that the investigator, or the individual responsible for the preliminary inquiry, bears the mandatory duty to ascertain, via investigative measures, that the injury wrought by the criminal offence has been comprehensively redressed. The authors delineate that the transcript of the victim's interrogation is the most prevalent procedural document, signifying that the conciliation procedures are mutually satisfactory, voluntary, and indicative of the parties' willingness and preparedness to reconcile. Conclusively, the authors infer that exemption from criminal accountability and the consequent cessation of the criminal case, predicated upon reconciliation or earnest remorse, is contingent upon the substantiated evidence of actual compensation for the harm engendered by the criminally punishable deed.
Индонезийские СМИ о специальной военной операции России (Indonesian media about Russia's special military operation)
Authors: Syurkani Panca , Muzykant Valerii L.
Published: November 2024

The Russian-Ukrainian military conflict often makes headlines in many media outlets around the world, including media in Indonesia. However, unfortunately, some media outlets publish fake news regarding the Russian-Ukrainian military conflict. This study examines fake news about Russia's special military operation in Indonesian media. The research method used is descriptive-qualitative. The data collection methodology was carried out by observing various Indonesian media outlets that published fake news related to the Russian-Ukrainian military conflict in Indonesian media. The collected data was then carefully analysed. Based on the analysis, it was established that several media outlets in Indonesia have been proven to disseminate false information regarding the Russian-Ukrainian military conflict that took place in 2022. While some of the above reports related to the Russian-Ukrainian army conflict have been proven fake news or hoaxes, the government does not impose sanctions on journalists or media outlets that publish such information. This is because journalists and media did not check the legitimacy of the information and did not fact-check. After verifying that the government has taken some steps to restore order in this area, the amount of fake news regarding the current Russian-Ukrainian military conflict has decreased significantly.
Classification of Circumstances that Contributed to the Commission of a Crime in the Legislation of the Russian Federation
Authors: Volkova Galina , Ivanov Dmitriy , Vasyukov Vitaliy , Polyakov Sergey , Batyukova Vera
Published: November 2024

In the article, the authors consider the most pressing issues related to the classification of circumstances that contributed to the commission of a crime in Russian legislation. It is established that the factors that make it possible to classify the above circumstances are: the identity of the suspect (accused); the living conditions and upbringing of the person who committed the crime; the circle of communication of the person who committed the crime; the circumstances of the origin of criminal intent; the circumstances that made it possible to achieve criminal goals; circumstances of an objective nature, the totality of which allows you to commit a specific crime; the absence of preventive measures and preventive work in the presence of appropriate conditions. The authors substantiate the position that the considered factors, according to which the classification of the circumstances under study was carried out, are conditional and are not exhaustive in their essence. At the same time, the classification of the circumstances under consideration by the authors allows the head of the investigative body and the investigator to correctly and competently plan their daily activities aimed at identifying, proving and eliminating them. In conclusion, the authors conclude that the classification of circumstances that contributed to the commission of a crime is necessary to build an algorithm of actions to identify, prove and eliminate them at all stages of pre-trial proceedings in criminal cases. Only when all three dominants are achieved (identification, proof, elimination) will the goals of criminal proceedings be achieved
Use of special knowledge in the investigation of crimes in the field of credit
Authors: Bondar Volodymyr , Korshun Anton , Chashnytska Tetiana , Bochkarova Maryna , Priakhin Yevhen , Nahornyi Andrii
Published: November 2024

On the basis of the results of the analysis of judicial and expert practice, as well as scientific sources, a study of the problematic aspects of the involvement of a specialist in the conduct of individual investigative (search) actions, the appointment and conduct of expert studies in the investigation of crimes in the field of lending was carried out. It has been established that the existence of schemes of criminal activity is typical for the technology of criminal enrichment through credit and financial transactions, which include: appropriation of bank credit resources, fraudulent obtaining of credit, fraud with financial resources. It was emphasized that the clarification of certain circumstances in the mentioned criminal proceedings is impossible without the use of special knowledge, primarily related to making changes to the relevant official documents. Typical investigative (search) actions are highlighted, in which it is necessary to actively use the help of a specialist, as well as the main areas of application of special knowledge by the parties to criminal proceedings, in particular, the investigator and the defense attorney. The importance of forensic examination (technical examination of documents, handwriting examination, complex forensic dactyloscopy, handwriting and technical examination of documents) is indicated as one of the main means of obtaining evidence during the investigation of criminal offenses in the field of lending. Particular attention is focused on determining the purpose, subject and tasks of technical examination of documents in the investigation of criminal offenses of this category.
Анализ Дела о Смерти Джамаля Хашогги: Перспективы Международного Права и Государственной Юрисдикции (Analysis of the Jamal Khashoggi Death Case: Perspectives on International Law and State Jurisdiction)
Authors: Faris Dzaka Ashriel , Fadillah Firman , Machyawaty Tety
Published: November 2024

The case of the murder of Jamal Khashoggi at the Saudi Consulate in Istanbul has drawn the attention of the international community to such important issues of international law as the abuse of diplomatic immunity and State responsibility for human rights violations. This analysis reveals the dynamics of international law and national jurisdiction in relation to crimes committed at diplomatic facilities, which should be protected by diplomatic immunity. The principle of territorial jurisdiction allows Turkey, as a crime scene, to investigate and bring the perpetrators to justice, while Saudi Arabia faces problems in complying with international conventions on law enforcement, fair treatment of foreign citizens and protection of human rights. The Khashoggi case raises questions about the extent to which diplomatic immunity can protect international crimes and how countries can fulfill their obligations under international law without sacrificing sovereignty. The impact of this event on Saudi Arabia's reputation and diplomatic relations is also significant, which has sparked a debate about the role of soft power and ethics in world politics. This study aims to deepen understanding of the challenges in the field of international justice, emphasizing the importance of States complying with existing international standards and responding decisively to the abuse of diplomatic immunity. The study concludes by emphasizing the need to reform international law as a preventive measure against such crimes in the future, while ensuring respect for the principles of justice at all levels.
Schaár Erzsébet ifjúkora és korai szobrászművészete (1908-1931)
Authors: Konkoly Ágnes
Published: November 2024

The present paper is a subchapter of my doctoral dissertation titled The Oeuvre of Sculptor Erzsébet Schaár (1908– 1975). The paper discussing a period of her life’s path sofar only tangentially touched on by special literature divides into three parts: the first is concerned with the artist’s family background, childhood and adolescence, the second with the initial phase of the career, the academy years, and the third looks at works created in the 1920s and the early 1930s, as well as exhibitions of that period.

Schaár was a veritable “child prodigy”. She began her studies as Zsigmond Kisfaludi Stróbl’s private pupil at the age of sixteen and a year later, in 1925 she was admitted to the Royal Academy of Art where her tutor was again Kisfaludi Stróbl. She debuted with her works publicly in 1926, first of all in exhibitions of the Szinyei Society and the KÚT (=new society of artists). Though several of her that-time works got lost or are latent, an approximately authentic overview of her art in that period can be had from exhibition catalogues, receptions, materials in archives and private collections – first of all the Vilt estate in the King St Stephen Museum in Székesfehérvár and the mostly unprocessed written and photography documents, correspondence in the Schaár estate, as well as from recollections by her contemporaries, first of all Tibor Vilt. A few nudes are known from this period, but her early artistic activity centred on portrait sculpture. This is also the clew along which the phases Schar’s artistic development, the modifications of her style, the influences exerted on her can be demonstrated from the earliest known portraits of her mother to the series of children’s portraits in the late 1920s, early 1930s. The studied period is characterized by a search of her own idiosyncratic style: in the 1920s she created both realistic portraits and works displaying features of art deco and cubism, she was interested in modelling with simple forms, experimented with facial casts – a procedure that returned in later phases of the oeuvre.

In sum, the earliest period of Schaár’s sculptural activity displays such singularly powerful idea of plasticity and creative outlook that compare with the later phases of the oeuvre in talent and quality, but with the help of which posterity can discern the trends that were to unfold in full later. Studying this early period may contribute to a more profound understanding and comprehensive interpretation of the entire oeuvre.
Adalékok Binder János Fülöp rézmetsző munkásságához
Authors: Tüskés Anna
Published: November 2024

Johann Philipp Binder (1736/7-1811), who worked in Buda in the second half of the 18th century and the first decade of the 19th, followed the Augsburg Rococo engraving tradition of the middle third of the 18th century. Since the publication of György Rózsa’s catalogue of three hundred and eighty-eight engravings by Binder and his workshop in 1998, his work has been the subject of much attention. In my study, I supplement the known oeuvre with nearly twenty signed engravings on religious and secular subjects found in nine books or other printed sources not yet examined in relation to Binder’s oeuvre, and discovered on three separate sheets. One of these may also modify the artist’s biography as known and accepted to date. On the basis of his will published five days after his death on 23 December 1811, I list the possessions he left to his second wife, who was still running the workshop a decade later. Museum catalogues and databases open up new perspectives for research. An important methodological lesson for the compilation of a new, more complete Binder catalogue, and for the processing of the 18th-century Hungarian reproduced graphic corpus in general, is that it is important to take all surviving copies of a book in hand, as not all illustrations are bound in all copies. Such a Binder catalogue would allow an analysis of the change in the artist’s style and the gradual inclusion of assistants.
Possible System Architecture for Travel Recommender
Authors: Supriyanto Supriyanto , Fahana Jefree
Published: November 2024

Travel recommender systems have been developed to meet the needs of users in the field of tourism. This system has several versions depending on the characteristics of the country, users and filtering techniques used. The development of recommendation filtering system techniques is very rapid so that the recommendation system has high enough complexity, but it also must have high usability. This paper discusses how the travel recommender system architecture is built by examining data structures, processing procedures and interaction design. The goal is to obtain the best usability in implementing a travel recommendation system. The system is built using the example case of finding the right tourist spot in Yogyakarta, Indonesia. This system applies several filtering techniques such as knowledge-based filtering, content-based filtering, and collaborative filtering. The evaluation results show that the system architecture optimized gets a usability level acceptable.
Investigation of shear mechanical behaviors of geogrid-coral sand interfaces
Authors: Luo Z. , Ding X. , Ou Q. , Zhang L. , Zhang T.
Published: November 2024

Geogrid-reinforced coral sand (GRCS) effectively enhances the strength and stability of marine geo-structures in island and coastal areas. The complex interaction and underlying mechanism of the geogrid-coral sand interface potentially determine the mechanical properties of the GRCS. In this study, large-scale interfacial shear tests were carried out to investigate the mechanical behaviors of geogrid-coral sand interfaces under the coupled influence of grain size, geogrid properties, and stress level. Comparisons with siliceous sand indicate that the mechanical properties of the geogrid-coral sand interface are excellent but suffer from more complex influences originating from particle properties. The interfacial friction angle is more sensitive to grain size, whereas the pseudo cohesion is more sensitive to aperture properties. The shear interface shows a general evolution trend of “contraction-dilation-contraction”, with the maximum and final dilation becoming more prominent as the aperture size and stress level decrease. The increase in grain size, vertical stress and geogrid apertures induces a thicker interface with more particle crushing. Given the microscopic geogrid-coral sand interaction, the force characteristics of the aperture are further analyzed, and an equivalent additional stress calculation method is developed to quantitatively characterize the effects of grain size, geogrid properties, and stress level.
Geotechnical characteristics of levees subjected to sand boil due to high-water levels
Authors: Kawajiri Shunzo , Kawaguchi Takayuki , Ogasawara Akinobu , Minabe Yuki , Nakamura Dai
Published: November 2024

The investigation of river levees holds significant implications for mitigating flood damage. Sand boiling, backward erosion piping, and phenomena manifesting along the riverside of levees directly imperil the integrity of these structures. It is imperative to address these phenomena comprehensively to safeguard both lives and property amidst flood events. The principal aim of this research is to delineate the variances in geotechnical conditions between sand boils observed at slope toes on the landside and those occurring at a distance from this region along the levee. Therefore, this study conducted extensive boring investigations at sites where sand boils occurred. The soil samples sampled from the boring investigations were analysed for grain size. The results of a series of geotechnical investigations showed that in the cases where sand boils occurred near the toe of the slope, a series of sandy soils with grain size characteristics similar to those of the sand boils were deposited in the foundation of the levee. On the other hand, in the case where the sand boil occurred far from the toe of the slope, sandy soil with grain size characteristics similar to that of the sand boil was deposited only on the landside.
From Simone de Beauvoir’s ‘House’ to bell hooks’ ‘Homeplace’: Autofiction and Autotheory in Architectural Writing
Authors: Cheatle Emma
Published: November 2024

From 1936 to 1948 Simone de Beauvoir lived alone in different hotels in Marseille, Rouen and Paris, from where she conceived her fictional and philosophical writing. In 1937, after convalescing in the south of France from a collapsed lung, she took a room in the hotel Mistral in Montparnasse to write the autofictional novel She Came to Stay (1943). The English writer Jean Rhys also lived an itinerant existence in successive, shabby Parisian hotels. Her novels written between 1928 and 1939 — Quartet, After Leaving Mr Mackenzie and Good Morning Midnight — are autofictional renditions of the desperate existence of a stateless, impoverished, unmarried woman.
Making close readings of de Beauvoir and Rhys texts, the article theorises that the in-between, material space of the early 20th-century hotel was integral to shaping an alternative domestic life for women which resisted patriarchal imperatives. Whilst promoting feminist autofiction as a source of evidence of the material conditions of women’s lives, I draw on Audre Lorde (1984) and others to critique the limitations of de Beauvoir and Rhys’ renditions with respect to class and race. Analysing the autotheoretical approach of bell hooks’ 1990 essay ‘Homeplace’, I suggest that autotheory is an alternative mode that can decolonise writing on domesticity and, further, politically extend architectural history writing.
Roundtable Discussion: Policy, Data and the UK's Hidden Screen Industries
Authors: Caston Emily
Published: November 2024

On 8th July 2024, industry leaders Steve Davies and Steve Garvey met at the British Film Institute (BFI) to discuss research and policy issues with the PI and Co-I of the AHRC Hidden Screen Industries network (Emily Caston and Patrick Russell), along with Ian Cade of the BFI’s Research and Statistics Unit. Topics covered included tax reliefs, screen skills, diversity and inclusion, the lack of research on advertising screen production and branded film, and priorities for future research. A selection of excerpts of the key points arising in the discussion are presented in this final contribution to the Special Dossier on Hidden Screen Industries.
The dialogue between philosophical schools on the «anthropological principle» in articles by N.G. Chernyshevsky and M.N. Katkov
Authors:   Saint Petersburg State University of Economics, Chernykh Andrey A.
Published: November 2024

The paper deals with the polemics between N.G. Chernyshevsky and M.N. Katkov on P.D. Yurkevich’s article «From the Science of the Human Spirit». In particular, the articles by M.N. Katkov «Old Gods and New Gods» and «Concerning “Polemical Beauties” in “Sovremennik”», published in «Russky Vestnik», and the work by N.G. Chernyshevsky «Polemical Beauties», published in «Sovremennik», are analyzed. By its form, the argument is closer to an altercation than to a scientific discussion, but the essence of the argument between N.G. Chernyshevsky and M.N. Katkov highlighted the philosophical basis for the differences between the theory of Chernyshevsky’s school and the views of M.N. Katkov and P.D. Yurkevich. The paper notes that the principle of partisanship in philosophy cannot be reduced only to the ontological division of thinkers into materialists and idealists, but also implies the epistemological distinction between monism and dualism. The discussion between M.N. Katkov and N.G. Chernyshevsky is considered as the special in which the universal is reflected. In other words, the episodic dispute between M.N. Katkov and N.G. Chernyshevsky expresses the main essence of the whole discussion of the 1860s on the anthropological principle. The paper outlines possible ways of overcoming the situation in which two unconnected monologues are conducted under the guise of a dialogue as well as offers the prospects for a philosophical dialogue between representatives of different philosophical schools and different points of view on fundamental questions of ontology and gnoseology. It is noted that in the 19th century in Russia materialism as a philosophical doctrine was less developed than idealism presented by the German classics, in connection with which this doctrine could be underestimated by opponents.
CONTRACT CRIMINAL PROCEDURE LAW AS A SUB-BRANCH OF MODERN RUSSIAN CRIMINAL PROCEDURE LAW
Authors:   Perm State University, Kolesnik V. V.
Published: November 2024

Abstract: in the Russian criminal procedural law, a sub-branch has been formed, which can be called contractual criminal procedural law. It includes criminal procedural proceedings, the basis of which is the agreement of the parties regarding the outcome of the case or its direction. This sub-branch of law is based on a system of its principles. These include the principles of speed, discretion, accusation, expediency, freedom of a person from self-disclosure, formal truth. The contractual criminal procedure mechanism serves as an addition to the general procedure for criminal proceedings. Its purpose is to achieve the general goals of criminal proceedings in an accelerated, simplified legal regime, saving the forces and means of public authority, as well as humanizing the criminal law impact. The article gives a general description of the new – contractual sub-branch of the modern criminal process in Russia and argues the need for its development.
LOST PROCEEDING ON CASES OF ADMINISTRATIVE OFFENCES IN COURT
Authors:   Perm State University, Bukhtoyarova О. А.
Published: November 2024

Abstract: in the process of the administrative law reform and creation “The Code of Administrative Judicial Procedure of the Russian Federation” in 2015, proceedings on cases of administrative offences in courts of general jurisdiction, which used to be in subsection III “Proceedings on cases lying in the sphere of the cases arising from public legal relationships” of “The Civil Procedure Code of the Russian Federation”, fell out of legal regulation, Till 2015, cases of administrative offences in courts of general jurisdiction were considered according to the rules of the above mentioned subsection of “The Civil Procedure Code of the Russian Federation” with particular aspects of “The Code of the Russian Federation on administrative offences”. But in 2015, due to withdrawing proceedings on cases of administrative offences arising from public legal relationships from one Code and creating a new Code on the basis of the previous one, the order of proceedings of cases of administrative offences was excluded from the newly-created Code of Administrative Judicial Procedure but not included in any other code judicial procedures.
Emma copy CUT&RUN: Targeted in situ genome-wide profiling with high efficiency for low cell numbers v1
Authors: Janssens Derek , Henikoff Steven
Published: November 2024

We previously described a novel alternative to Chromatin Immunoprecipitation, Cleavage Under Targets & Release Using Nuclease (CUT&RUN), in which unfixed permeabilized cells are incubated with antibody, followed by binding of a Protein A-Micrococcal Nuclease (pA/MNase) fusion protein. Upon activation of tethered MNase, the bound complex is excised and released into the supernatant for DNA extraction and sequencing. In the manuscript attached to this version of the protocol, Meers et al. introduce four enhancements to CUT&RUN: 1) a hybrid Protein A-Protein G-MNase construct that expands antibody compatibility; 2) a modified digestion protocol that prevents premature release of the nuclease-bound complex; 3) a calibration strategy based on carry-over of E. coli DNA introduced with the fusion protein; and 4) a novel peak-calling strategy customized for the low-background profiles obtained using CUT&RUN. Here we provide an updated CUT&RUN protocol that incorporates these enhancements, and provides three different options for the CUT&RUN MNase digestion reaction that are helpful to improve data quality or to increase throughput. These new features, coupled with the previously described low-cost, high efficiency, high reproducibility and high-throughput capability of CUT&RUN make it the method of choice for routine epigenomic profiling.
Stable cell line generation via retrovirus v1
Authors: Tudorica Dan , Velecela Victor
Published: November 2024

Production of pseudotyped virus an subsequent transduction.
Collections Standard Operating Protocol, Organism group: Lichens v1
Authors: L Jones Amanda , L Forrest Laura , Hart Michelle , Bell David , Yahr Rebecca
Published: November 2024

This is part of the collection "DToL Taxon-specific Standard Operating Procedure (SOP) for the Plant Working Group". The SOP collection contains guidance on how to process the various land plant taxa and lichens within the scope of the Darwin Tree of Life (DToL) project. The guidance specifically refers to the collection and processing of tissue samples needed for DNA barcoding (which takes place at the Royal Botanic Garden Edinburgh (RBGE)) and outlines the flash frozen tissues required for whole genome sequencing (WGS), which takes place at the Wellcome Sanger Institute. Every specimen is submitted for DNA barcoding first, before potentially being sent to the Wellcome Sanger institute. This Sample Collection SOP outlines the collection of lichen samples for the DToL project, which aims to generate high quality genome sequences from these samples. To achieve this goal, the DToL Genome Acquisition Labs (GALs) must collect a sufficient quantity of healthy living material, preserve it in a manner that conserves its DNA quality, and supply it to the appointed sequencing facility. Material must also be available and appropriately preserved for DNA barcoding and the provision of herbarium vouchers. It is the responsibility of the GALs to link accurate and information-rich metadata to all collections. Definition: Lichens Including: Lichenized Ascomycota and Basidiomycota (Agaricomycetes) Excluding: Non-lichenized fungi
Native Barcoding of amplicons (96 well plates) v1
Authors: Shaw Alex , Troman Catherine , Akello Joyce , Martin Javier , Grassly Nick , Bujaki Erika
Published: November 2024

The following protocol is for the preparation of amplicons for sequencing using the Oxford Nanopore Native barcoding kit version 14 with 96 barcodes (SQK-NBD114.96). When using this kit, you must ensure to use the appropriate flow cell (FLO-MIN114, R10.4.1) otherwise you will face difficulty when starting the run and sequencing results will not be accurate. We provide estimates of ng of material required for this protocol when amplifying the VP1 region of polio, the capsid region (PanEV) or the whole genome (PanPV). If you have amplicons of a different size to these, you can calculate the ng required using an online calculator such as this: Weight to Molar Quantity (for nucleic acids) (bioline.com)
A Worker’s Suicide as the Collapse of Masculinity: The Analysis of The Movie "My Father's Wings”
Authors: Çınar Sidar
Published: November 2024

My Father's Wings by Kıvanç Sezer (2016) shows us how loneliness and desperation are experienced, based on a case of a Turkish construction worker. This article analyzes the film My Father's Wings using the film sociology methodology and focusing on gender and the approach of the Turkish cinema industry that has emphasized emotions after the 2000s. The intertwined experiences of masculinity crisis and being a worker are discussed by articulating Turkish cinema and Turkey's social and political dynamics in the analysis of the film. Based on the film, the collapse of the two pillars on which masculinity is built as the head of the family and the master worker and individualization in the working class and society in general are discussed within the political and social atmosphere that resulted in everyone's withdrawal to their own world.
Maritime Justice, Environmental Crime Prevention, and Sustainable Development Goal 14
Authors: McLaughlin Conor , Lothian Sarah , Lindley Jade
Published: November 2024

SDG 14 “life below water” sets targets for the conservation and sustainable use of the oceans, seas, and marine resources, however, it is increasingly evident that its targets are unachievable without strengthened state‐led maritime justice. This article examines the concept of “maritime justice” from an environmental crime perspective and explores the critical link between maritime justice and ocean crime resilience. The article addresses the relationship between maritime justice and SDG 14 and explores various approaches to progress maritime justice in order to better respond to environmental crimes at sea.
Economic analysis of implementing Systemic Coronary Risk Estimation (SCORE2) scale and long-term consequences
Authors: Kurilovich E. O. , Nikitina A. V. , Sorokovikov I. V.
Published: November 2024

Objective: to assess the demand for pharmacotherapy in risk groups on adverse cardiovascular (CV) events identified during prophylactic medical examination of the adult population according to the SCORE2 (Systemic Coronary Risk Estimation) scale, which is being introduced into clinical practice.

Material and methods. To analyze the economic consequences of including diagnostic examinations to assess the risk by SCORE and SCORE2 scales, a Markov model was developed, which described the course of lipid metabolism disorders in adult patients aged 40 to 69 years without concomitant diseases identified during prophylactic medical examination. The model cycle was 2 months (the average waiting time for response to therapy); the modeling horizon was 3 years; the calculated values were estimated for the period 2024–2026. The economic analysis of long-term consequences involved calculating the cost of pharmacotherapy and the costs associated with CV events at outpatient and inpatient stages of medical care. The costs of drug therapy were determined based on dosage regimens presented in instructions for medical use in Russia, and clinical guidelines.

Results. According to the modeling results, in the period 2024–2026, the administration of pharmacotherapy to reduce the risks of CV events will enable to achieve 23,224 potentially saved lives through exclusively government funding or 23,605 through mixed sources of funding (government and citizens’ own funds). The cost of the analyzed pharmacotherapy upon the introduction of the SCORE2 scale will be from 50.18 billion rubles (with government funding) to 318.14 billion rubles. Concurrently, pharmacocorrection will provide a reduction in the costs of inpatient treatment and outpatient medical services to 4.1 billion rubles due to the achieving of intended low-density lipoprotein level, and 1.6 billion rubles due to the achieving of intended triglyceride levels.

Conclusion. Using new SCORE2 scale may lead to changes in the practice of prescribing lipid-lowering prophylaxis of CV diseases, as well as to a sharp increase in the number of patients who may require earlier prescription of pharmacotherapy to prevent CV events.
The impact of CTLA-4 gene polymorphism on the content of organ-specific antibodies in patients with autoimmune thyroiditis among the population of Azerbaijan
Authors: Rahimova Rena R. , Dashdamirova Gulnara S. , Guliyeva Sabina R. , Azizova Ulviyya H. , Rzayeva Farkhanda F.
Published: November 2024

BACKGROUND: The risk of developing immune tolerance to self-antigens in autoimmune thyroiditis is largely associated with a mutation in the CTLA-4 gene, the product of which negatively regulates T-cell activity.
AIM: To study the A49G (rs231775) CTLA-4 polymorphism in patients with autoimmune thyroiditis in a sample of Azerbaijani residents and the effect of the frequency of identified alleles and genotypes on the level of antibodies to thyroid peroxidase and thyroglobulin.
MATERIAL AND METHODS: A study was conducted in 2021–2023 involving 170 patients with autoimmune thyroiditis and 65 individuals without thyroid pathology or other autoimmune diseases, who formed a comparison group. The groups were matched by gender and age. Based on the patient history, thyroid status study results, thyroid ultrasound data, and organ-specific antibody assessment, the patients were diagnosed with autoimmune thyroiditis. The routine thyroid status study results in the comparison group were within normal limits. Genotyping of the A49G (rs231775) polymorphism was performed using polymerase chain reaction followed by digestion of the reaction product with PspEI restriction endonuclease. The results of the quantitative studies were presented as the median (Me) and interquartile range (25th quartile; 75th quartile). The correspondence of genotype frequencies to the Hardy–Weinberg equilibrium was assessed using the χ2 criterion.
RESULTS: The results revealed a statistically significant increase in the frequency of the G allele (48%) compared to the control group [33.8%; p=0.039, χ2=4.27, odds ratio (OR) 1.865, 95% confidence interval (CI) 1.028–3.382] and a decrease in the frequency of the A allele (51.2%) relative to the control group (66.1%; p=0.0389, χ2=4.27, OR=0.536, CI=0.296–0.973). In 22.4% of patients with the GG genotype (p=0.005, χ2=7.86, OR=0.237, 95% CI=0.088–0.635) and 55.6% of patients with the G allele (p=0.0012, χ2=10.43, OR=0.360, 95%, CI=0.192–0.674), thyroglobulin antibody titers were more than 100 IU/ml. Thyroid peroxidase antibody levels of 100 IU/ml or more were recorded in 22.7% of study participants with the GG genotype (p=0.030) and 50.0% with the G allele (p=0.048).
CONCLUSION: Elevated titers of antibodies to thyroid peroxidase and thyroglobulin were detected in patients from the population of Azerbaijan with the G allele and homozygous genotype GG of the +49A/G polymorphism of the CTLA-4 gene.
Caregiver-Observer Agreement of Emotion Expressivity in Autistic and Typically Developing Young Children
Authors:   , Register Hannah McKinnon , Wieckowski Andrea T.
Published: November 2024

Emotion expression is thought to present differently in autistic individuals and differences in presentation can be an early indicator of autism. However, there is no clear consensus on the measurement of emotion expressivity in early childhood. Caregiver report may provide a cost effective and time efficient method to measure emotion expressivity, while trained observers may report expressivity with an objective lens, but within a specific context. This study aims to investigate the level of agreement between caregivers and trained observers regarding emotion expression in both autistic and typically developing young children. Forty-nine children (27 diagnosed with autism spectrum disorder, 22 typically developing) with a mean age of 41.94 months (SD = 11.25) were assessed for facial expressions of joy and frustration through a play-based temperament battery, and caregivers completed emotion expression questionnaires. Caregivers of autistic children were more likely to report differences from expectation in how their child expressed emotions, compared to caregivers of typically developing children, and trained observers found lowered frequency of joy during play-based tasks. Overall, low caregiver-trained observer agreement was found in both typically developing and autistic groups, with no group differences in agreement identified. There was moderate agreement between caregivers and trained observers on decreased positive emotion expressivity, suggesting a potential avenue to measure attenuated positive emotion expressivity in autistic young children. These findings contribute to our current knowledge of multi-method, multi-informant agreement regarding emotion expressivity and provide future avenues of research to pursue regarding atypical emotion expression as an early indicator of autism spectrum disorder.
Humanity in the Eyes of Androids: A Few Reflections about the Book “Klara and the Sun” by Kazuo Ishiguro
Authors: Gajak-Toczek Małgorzata
Published: November 2024

The novel Klara and the Sun by Kazuo Ishiguro introduces the reader to a world immersed in information technologies. The author of the article presents reflections on the coexistence of human beings and humanoids. After presenting the title robot and her path of maturation to her own identity, considerations are made on the technological “improvement” of human being (genetic engineering) and human aspirations to avoid death (immortalism). The final part contains didactic recommendations – several selected fields of consideration that are worth taking with students as future teachers are indicated.
Hepatoprotective Effect of Xymedon and its Conjugate with L-ascorbic Acid during Poisoning of Mice with a Semilethal Dose of Paracetamol
Authors: Belyaev G.P. , Vyshtakalyuk A.B. , Parfenov A.A. , Galyametdinova I.V. , Semenov V.E. , Zobov V.V.
Published: November 2024

There is currently an urgent need to develop effective therapies aimed at preventing the consequences of drug-induced liver injury (DILI). In this context, our study focused on assessing the hepatoprotective effect of pyrimidine derivatives of xymedon (1,2-dihydro-4,6-dimethyl-1-(2- hydroxyethyl)-pyrimidin-2-one) and its conjugate with L-ascorbic acid in a mouse model of paracetamol (acetaminophen) poisoning. ICR (CD-1) mice were treated with a single oral LD50 dose of paracetamol (425 mg/kg), followed by intraperitoneal injections of various doses of the test compounds at various doses half an hour later. The animals` survival was monitored for 5 days. On the day 6, biochemical and histopathological evaluations of the effects of the test compounds were conducted. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities were analyzed. Malondialdehyde (MDA) levels in blood and liver tissue were also determined. Xymedon attenuated the toxic effects of paracetamol on the liver, improving animal survival and enhancing the macro- and micro-structure of the liver, normalizing the biochemical indicator of liver condition ALT and the serum marker of oxidative stress MDA. The conjugate of xymedon with L-ascorbic acid exhibited a more pronounced hepatoprotective effect compared to the native xymedon molecule.
Calculadoras Cifra: una experiencia pionera en diseño y producción electrónica en América Latina (Argentina, 1969-1976)
Authors: Urcelay Florencia Etel , Cáceres Yamila Noely , Picabea Juan Facundo
Published: November 2024

A fines de la década de 1960, una empresa privada de capitales nacionales emprendió un proceso de diversificación tecno-productiva y lideró el diseño y fabricación de calculadoras electrónicas en Argentina. Este artículo analiza la trayectoria de la Fábrica Argentina de Telas Engomadas (fate) durante la segunda fase del modelo de sustitución de importaciones y de dos centros de investigación pertenecientes a la Universidad de Buenos Aires: el Instituto de Cálculo de la Facultad de Ciencias Exactas y el Departamento de Electrónica de la Facultad de Ingeniería. Asimismo, analiza cómo el golpe de Estado de 1966 promovió la convergencia de dichas trayectorias y la creación de la División Electrónica de fate. Finalmente, analiza el desarrollo de tecnologías conocimiento-intensivas en un país semiperiférico.