bluuebunny/crossref_metadata_2025 huggingface dataset:
The Infratentorial Localization of Brain Metastases May Correlate with Specific Clinical Characteristics and Portend Worse Outcomes Based on Voxel-Wise Mapping
Authors: Dou Zhangqi , Wu Jiawei , Wu Hemmings , Yu Qian , Yan Feng , Jiang Biao , Li Baizhou , Xu Jinghong , Xie Qi , Li Chenguang , Sun Chongran , Chen Gao
Published: January 2021

The infratentorial regions are vulnerable to develop brain metastases (BMs). However, the associations between the infratentorial localization of BMs and clinical characteristics remained unclear. We retrospectively studied 1102 patients with 4365 BM lesions. Voxel-wise mapping of MRI was applied to construct the tumor frequency heatmaps after normalization and segmentation. The analysis of differential involvement (ADIFFI) was further used to obtain statistically significant clusters. Kaplan-Meier method and Cox regression were used to analyze the prognosis. The parietal, insular and left occipital lobes, and cerebellum were vulnerable to BMs with high relative metastatic risks. Infratentorial areas were site-specifically affected by the lung, breast, and colorectal cancer BMs, but inversely avoided by melanoma BMs. Significant infratentorial clusters were associated with young age, male sex, lung neuroendocrine and squamous cell carcinomas, high expression of Ki-67 of primaries and BMs, and patients with poorer prognosis. Inferior OS was observed in patients with ≥3 BMs and those who received whole-brain radiotherapy alone. Infratentorial involvement of BMs was an independent risk factor of poor prognosis for patients who received surgery (p = 0.023, hazard ratio = 1.473, 95% confidence interval = 1.055–2.058). The current study may add valuable clinical recognition of BMs and provide references for BMs diagnosis, treatment evaluation, and prognostic prediction.
Comparative Studies on the Susceptibility of (R)-2,3-Dipalmitoyloxypropylphosphonocholine (DPPnC) and Its Phospholipid Analogues to the Hydrolysis or Ethanolysis Catalyzed by Selected Lipases and Phospholipases
Authors: Mituła Paweł , Wawrzeńczyk Czesław , Gładkowski Witold
Published: January 2021

Susceptibility of soybean phosphatidylcholine, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and its phosphono analogue (R)-2,3-dipalmitoyloxypropylphosphonocholine (DPPnC) towards selected lipases and phospholipases was compared. The ethanolysis of substrates at sn-1 position was carried out by lipase from Mucor miehei (Lipozyme®) and lipase B from Candida antarctica (Novozym 435) in 95% ethanol at 30 °C, and the hydrolysis with LecitaseTM Ultra was carried out in hexane/water at 50 °C. Hydrolysis at sn-2 position was carried out in isooctane/Tris-HCl/AOT system at 40 °C using phospholipase A2 (PLA2) from porcine pancreas and PLA2 from bovine pancreas or 25 °C using PLA2 from bee venom. Hydrolysis in the polar part of the studied compounds was carried out at 30 °C in acetate buffer/ethyl acetate system using phospholipase D (PLD) from Streptococcus sp. and PLD from white cabbage or in Tris-HCl buffer/methylene chloride system at 35 °C using PLD from Streptomyces chromofuscus. The results showed that the presence of C-P bond between glycerol and phosphoric acid residue in DPPnC increases the rate of enzymatic hydrolysis or ethanolysis of ester bonds at the sn-1 and sn-2 position and decreases the rate of hydrolysis in the polar head of the molecule. The most significant changes in the reaction rates were observed for reaction with PLD from Streptococcus sp. and PLD from Streptomyces chromofuscus that hydrolyzed DPPnC approximately two times slower than DPPC and soybean PC. The lower susceptibility of DPPnC towards enzymatic hydrolysis by phospholipases D gives hope for the possibility of using DPPnC-like phosphonolipids as the carriers of bioactive molecules that, instead of choline, can be bounded with diacylpropylphosphonic acids (DPPnA).
Solution NMR Structure of the SH3 Domain of Human Caskin1 Validates the Lack of a Typical Peptide Binding Groove and Supports a Role in Lipid Mediator Binding
Authors: Tőke Orsolya , Koprivanacz Kitti , Radnai László , Merő Balázs , Juhász Tünde , Liliom Károly , Buday László
Published: January 2021

SH3 domains constitute an important class of protein modules involved in a variety of cellular functions. They participate in protein-protein interactions via their canonical ligand binding interfaces composed of several evolutionarily conserved aromatic residues forming binding grooves for typical (PxxP) and atypical (PxxxPR, RxxK, RKxxY) binding motifs. The calcium/calmodulin-dependent serine protein kinase (CASK)-interacting protein 1, or Caskin1, a multidomain scaffold protein regulating the cortical actin filaments, is enriched in neural synapses in mammals. Based on its known interaction partners and knock-out animal studies, Caskin1 may play various roles in neural function and it is thought to participate in several pathological processes of the brain. Caskin1 has a single, atypical SH3 domain in which key aromatic residues are missing from the canonical binding groove. No protein interacting partner for this SH3 domain has been identified yet. Nevertheless, we have recently demonstrated the specific binding of this SH3 domain to the signaling lipid mediator lysophospatidic acid (LPA) in vitro. Here we report the solution NMR structure of the human Caskin1 SH3 domain and analyze its structural features in comparison with other SH3 domains exemplifying different strategies in target selectivity. The key differences revealed by our structural study show that the canonical binding groove found in typical SH3 domains accommodating proline-rich motifs is missing in Caskin1 SH3, most likely excluding a bona fide protein target for the domain. The LPA binding site is distinct from the altered protein binding groove. We conclude that the SH3 domain of Caskin1 might mediate the association of Caskin1 with membrane surfaces with locally elevated LPA content.
Autophagy Involvement in the Postnatal Development of the Rat Retina
Authors: Pesce Noemi Anna , Canovai Alessio , Lardner Emma , Cammalleri Maurizio , Kvanta Anders , André Helder , Dal Monte Massimo
Published: January 2021

During retinal development, a physiologic hypoxia stimulates endothelial cell proliferation. The hypoxic milieu warrants retina vascularization and promotes the activation of several mechanisms aimed to ensure homeostasis and energy balance of both endothelial and retinal cells. Autophagy is an evolutionarily conserved catabolic system that contributes to cellular adaptation to a variety of environmental changes and stresses. In association with the physiologic hypoxia, autophagy plays a crucial role during development. Autophagy expression profile was evaluated in the developing retina from birth to post-natal day 18 of rat pups, using qPCR, western blotting and immunostaining methodologies. The rat post-partum developing retina displayed increased active autophagy during the first postnatal days, correlating to the hypoxic phase. In latter stages of development, rat retinal autophagy decreases, reaching a normalization between post-natal days 14-18, when the retina is fully vascularized and mature. Collectively, the present study elaborates on the link between hypoxia and autophagy, and contributes to further elucidate the role of autophagy during retinal development.
The Relationship between the Dominant Hand and the Occurrence of the Supracondylar Humerus Fracture in Pediatric Orthopedics
Authors: Herdea Alexandru , Ulici Alexandru , Toma Alexandra , Voicu Bogdan , Charkaoui Adham
Published: January 2021

It is known that during a fall, a child would rather protect their dominant hand by using the non-dominant one, although the role of handedness in upper limb fractures has not been studied in-depth. We carried out a retrospective, cross-sectional cohort study, including pediatric patients who presented to the emergency room with a supracondylar humerus fracture following an injury by falling from the same height. In total, 245 patients were selected and grouped according to age. In the 1–3 years group, no statistical significance was found between hand dominance and the side of fracture (p = 0.7315). During preschool years (4–6 years old), the non-dominant hand is more often involved (p = 0.03, odds ratio: 3.5). In the 7–14 years group this trend was maintained and actually increased (p = 0.052, odds ratio: 3.8). We might conclude that children tend to protect their dominant hand by falling on their non-dominant one. The main objective of this study is to highlight a link between handedness and the side of the body where the hand fracture will be identified in the pediatric population, regarding supracondylar humerus fracture.
Rapid Realist Review of School-Based Physical Activity Interventions in 7- to 11-Year-Old Children
Authors: Defever Emmanuel , Jones Michelle
Published: January 2021

Meta-analysis of physical activity interventions in school settings have revealed low efficacy and that there is a need to explore implementation fidelity. The aim of this rapid realist review was to determine, what physical activity interventions in school settings for children aged 7- to 11-years-old works, for whom, and in what circumstances. The realist synthesis was conducted following RAMESES guidelines. Relevant studies were identified following a systematic search process and data from 28 studies was extracted for evidence to form context-mechanism-outcome configurations that were clustered and refined. Using the five-level socioecological model, the program theories were classified into the levels of intrapersonal (child), interpersonal (teachers), institutional (program content, school administration, and school environment), community (home and neighborhood), and policy. The school level led to most context-mechanism-outcome configurations related to school leadership and policy, workforce structure, program characteristics, and school environment. At each level, we identified features of interventions, alongside implementation considerations that might work to promote efficacy and sustainability. The need to recognize the school environment as part of a complex system with multi-level interaction and influences was a key finding. In line with realist philosophy, the researchers encouraged primary research to confirm, refute, and refine the program theories presented.
Insights into the Frequency and Distinguishing Features of Sleep Disorders in Pediatric Palliative Care Incorporating a Systematic Sleep Protocol
Authors: Dreier Larissa Alice , Zernikow Boris , Stening Kathrin , Wager Julia
Published: January 2021

Currently, no concrete figures on sleep disorders and sleep characteristics in children and adolescents with life-limiting conditions (LLC) and severe neurological impairment (SNI) based on pediatric palliative care professionals’ assessment and following an official classification system such as the International Classification of Sleep Disorders (ICSD-3) exist. The ICSD-3 sleep disorders of inpatient children and adolescents with LLC and SNI (N = 70) were assessed by professionals using a recording sheet (two-year recruitment period). A systematic sleep protocol was applied to identify patients’ sleep characteristics. Of these patients, 45.6% had sleep disorders, with the majority of them experiencing two different ones. Overall, the most frequently identified disorders were Chronic Insomnia and Circadian Sleep–Wake Disorder. Patients experiencing Chronic Insomnia showed more sleep phases during the daytime and more waking phases at nighttime than those unaffected. Patients with and without a Circadian Sleep–Wake Disorder additionally differed in the length of sleep phases during the daytime. Rapid changes between wakefulness and sleep were specifically characteristic of Hypersomnia. The study provides important insights into the prevalence and characteristics of individual ICSD-3 sleep disorders in pediatric palliative care. The findings may contribute to a targeted and efficient diagnosis and therapy of distressing sleep problems in seriously ill patients.
A Comparison Study on Multilayered Barrier Oxide Structure in Charge Trap Flash for Synaptic Operation
Authors: Kim Minkyung , Park Eunpyo , Kim In Soo , Park Jongkil , Kim Jaewook , Jeong YeonJoo , Lee Suyoun , Kim Inho , Park Jong-Keuk , Seong Tae-Yeon , Kwak Joon Young
Published: January 2021

A synaptic device that contains weight information between two neurons is one of the essential components in a neuromorphic system, which needs highly linear and symmetric characteristics of weight update. In this study, a charge trap flash (CTF) memory device with a multilayered high-κ barrier oxide structure on the MoS2 channel is proposed. The fabricated device was oxide-engineered on the barrier oxide layers to achieve improved synaptic functions. A comparison study between two fabricated devices with different barrier oxide materials (Al2O3 and SiO2) suggests that a high-κ barrier oxide structure improves the synaptic operations by demonstrating the increased on/off ratio and symmetry of synaptic weight updates due to a better coupling ratio. Lastly, the fabricated device has demonstrated reliable potentiation and depression behaviors and spike-timing-dependent plasticity (STDP) for use in a spiking neural network (SNN) neuromorphic system.
Mobile-Based Sensing Scheme to Minimize Battery Power Consumption for Urban Monitoring Systems
Authors: Lee Sang-Hoon , Yang Taehun , Kim Tae-Sung
Published: January 2021

In urban monitoring systems, mobile sensing is imperative to acquire data from sensors and relay them to a cloud server. Mobile devices can be used anytime and anywhere, enabling communication with pervasive sensing in various conditions to obtain the data. Reliable data acquisition has been required in urban monitoring systems from the macroscale to the microscale. However, a broadcast method for the data acquisition process may lead to the increased battery power consumption of mobile devices. Managing the battery power consumption of mobile devices is essential for reliable data acquisition. In this paper, we propose an urban monitoring system with an optimization algorithm in which a cloud server broadcasts a communication request that includes battery power consumption and the data acquisition quantity of mobile devices. Game theoretic optimization is formulated with a decision process. We derive a best response and Nash equilibrium for mobile communication with sensors and a cloud server. Evaluation results demonstrate that the proposed system can guarantee a low battery power consumption, as well as acquire the desired data quantity.
Algorithmic-Level Approximate Tensorial SVM Using High-Level Synthesis on FPGA
Authors: Younes Hamoud , Ibrahim Ali , Rizk Mostafa , Valle Maurizio
Published: January 2021

Approximate Computing Techniques (ACT) are promising solutions towards the achievement of reduced energy, time latency and hardware size for embedded implementations of machine learning algorithms. In this paper, we present the first FPGA implementation of an approximate tensorial Support Vector Machine (SVM) classifier with algorithmic level ACTs using High-Level Synthesis (HLS). A touch modality classification framework was adopted to validate the effectiveness of the proposed implementation. When compared to exact implementation presented in the state-of-the-art, the proposed implementation achieves a reduction in power consumption by up to 49% with a speedup of 3.2×. Moreover, the hardware resources are reduced by 40% while consuming 82% less energy in classifying an input touch with an accuracy loss less than 5%.
Anamorphic Site Index Curves for Central Appalachian Red Spruce in West Virginia, USA
Authors: Yetter Eric , Brown John , Chhin Sophan
Published: January 2021

Traditional site index curves are frequently produced for shade-intolerant species but are scarce for shade-tolerant species. Red spruce (Picea rubens Sarg.) can be found in three distinct geographic regions (northern, central, and southern) within the Appalachian Mountains. The one commonly used set of red spruce site index curves is over ninety years old. A definite need exists for a modern, regionally applicable set of site index curves. This research sampled 83 plots randomly located in the central Appalachians of West Virginia. Three sets of anamorphic site index curves were created after careful examination of height models built using Chapman-Richards and Meyer functions. One set of curves was constructed with traditional age height pairs. The second utilized a suppression-corrected age and height pair. The third set examined diameter at breast height (DBH) and height pairs. Fit statistics indicated better performance for the suppression-corrected age–height pair site index and the DBH–height pair site index versus the traditional age–height pair models. Site index conversion equations were also investigated for the red spruce age-corrected site index. Linear regression was used to determine significant geographic and climate variables and the utility of including site index values for red maple (Acer rubrum L.) and yellow birch (Betula alleghaniensis Britton) in the model to predict red spruce site index. Significant models were found for varying combinations of species site index, climate, and geographic variables with R2adj in the range of 0.139–0.455. These new site index curves and conversion equations should provide utility for site productivity estimation and growth and yield modeling while aiding in restoration efforts for this important central Appalachian species.
On the Consequences of Using Moving Window Segmentation to Analyze the Structural Stand Heterogeneity and Debatable Patchiness of Old-Growth Temperate Forests
Authors: Paluch Jarosław
Published: January 2021

(1) Background: Early research in natural forests on decennia implanted conviction concerning the patchy patterns of their structural heterogeneity. Due to the variety of methodological approaches applied, verification of this fundamental assumption remains open. The aim of this study was to discuss the methodological limitations associated with the use of moving windows with overlap for the delineation of homogeneous patch mosaics in forest ecosystems. (2) Methods: The “patchiness” hypothesis was tested in six old-growth forests formed by Abies alba Mill., Fagus sylvatica L., and Picea abies (L.) H. Karst. localized in Bosnia and Herzegovina and southern Poland. In each stand, the tree diameter at breast height (dbh) was recorded on circular sample plots of 154 m2 regularly distributed in a 20 × 20 m lattice over an area of 10 ha. (3) Results: Computer simulations showed that patch classification based on overlapping windows results in apparent patchiness, even for completely randomized tree distributions. Analyses carried out on the empirical data indicated prevalent random patterns of structural heterogeneity. (4) Conclusions: Patchiness is not a universal feature of the investigated forest communities. The size of the moving window and the noise-smoothing procedure exert strong effects on the biasedness of patch classification, the frequency of structural types, and the mean patch size.
The Perceived Impact of Social Networking Sites and Apps on the Social Capital of Saudi Postgraduate Students: A Case Study
Authors: Alghamdi Abdulelah A. , Plunkett Margaret
Published: January 2021

With the increased use of Social Networking Sites and Apps (SNSAs) in Saudi Arabia, it is important to consider the impact of this on the social lives of tertiary students, who are heavy users of such technology. A mixed methods study exploring the effect of SNSAs use on the social capital of Saudi postgraduate students was conducted using a multidimensional construct of social capital, which included the components of life satisfaction, social trust, civic participation, and political engagement. Data were collected through surveys and interviews involving 313 male and 293 female postgraduate students from Umm Al-Qura University (UQU) in Makkah. Findings show that male and female participants perceived SNSAs use impacting all components of social capital at a moderate and mainly positive level. Correlational analysis demonstrated medium to large positive correlations among components of social capital. Gender differences were not evident in the life satisfaction and social trust components; however, females reported more involvement with SNSAs for the purposes of political engagement while males reported more use for civic participation, which is an interesting finding, in light of the norms and traditional culture of Saudi society.
Genetic Admixture in the Population of Wild Apple (Malus sieversii) from the Tien Shan Mountains, Kazakhstan
Authors: Ha Young-Ho , Oh Seung-Hwan , Lee Soo-Rang
Published: January 2021

There is growing attention given to gene flow between crops and the wild relatives as global landscapes have been rapidly converted into agricultural farm fields over the past century. Crop-to-wild introgression may advance the extinction risks of rare plants through demographic swamping and/or genetic swamping. Malus sieversii, the progenitor of the apple, is exclusively distributed along the Tien Shan mountains. Habitat fragmentation and hybridization between M. sieversii and the cultivated apples have been proposed to be the causal mechanism of the accelerated extinction risk. We examined the genetic diversity pattern of eleven wild and domesticated apple populations and assessed the gene flow between M. sieversii and the cultivated apples in Kazakhstan using thirteen nuclear microsatellite loci. On average, apple populations harbored fairly high within-population diversity, whereas population divergences were very low suggesting likely influence of human-mediated dispersal. Assignment results showed a split pattern between the cultivated and wild apples and frequent admixture among the apple populations. Coupled with the inflated contemporary migration rates, the admixture pattern might be the signature of increased human intervention within the recent past. Our study highlighted the prevalent crop to wild gene flow of apples occurring in Kazakhstan, proposing an accelerated risk of genetic swamping.
An Evidence-Based Strategic Approach to Prevention and Treatment of Dry Eye Disease, a Modern Global Epidemic
Authors: Donthineni Pragnya R. , Shanbhag Swapna S. , Basu Sayan
Published: January 2021

Dry eye disease (DED) is an emerging health concern causing significant visual, psychological, social, and economic impact globally. In contrast to visual rehabilitation undertaken at late stages of DED, measures instituted to prevent its onset, establishment, or progression can alter its natural course and effectively bring down the associated morbidity. This review attempts to present the available literature on preventive strategies of DED at one place, including strategies for risk assessment and mitigation, targeting a wide range of population. A literature search was conducted using PubMed and an extensive literature review on preventive strategies for DED was compiled to put forth a holistic and strategic approach for preventing DED. This can be undertaken at various stages or severity of DED directed at different tiers of the health care system. Conclusion: This review intends to put emphasis on preventive strategies being adopted as an integral part of routine clinical practice by general ophthalmologists and specialists to tackle the burden of DED and improve the quality of the lives of the patients suffering from it.
Current Issues on Research Conducted to Improve Women’s Health
Authors: Siristatidis Charalampos , Karageorgiou Vasilios , Vogiatzi Paraskevi
Published: January 2021

There are varied lessons to be learned regarding the current methodological approaches to women’s health research. In the present scheme of growing medical literature and inflation of novel results claiming significance, the sheer amount of information can render evidence-based practice confusing. The factors that classically determined the impact of discoveries appear to be losing ground: citation count and publication rates, hierarchy in author lists according to contribution, and a journal’s impact factor. Through a comprehensive literature search on the currently available data from theses, opinion, and original articles and reviews on this topic, we seek to present to clinicians a narrative synthesis of three crucial axes underlying the totality of the research production chain: (a) critical advances in research methodology, (b) the interplay of academy and industry in a trial conduct, and (c) review- and publication-associated developments. We also provide specific recommendations on the study design and conduct, reviewing the processes and dissemination of data and the conclusions and implementation of findings. Overall, clinicians and the public should be aware of the discourse behind the marketing of alleged breakthrough research. Still, multiple initiatives, such as patient review and strict, supervised literature synthesis, have become more widely accepted. The “bottom-up” approach of a wide dissemination of information to clinicians, together with practical incentives for stakeholders with competing interests to collaborate, promise to improve women’s healthcare.
UV Lighting in Horticulture: A Sustainable Tool for Improving Production Quality and Food Safety
Authors: Loconsole Danilo , Santamaria Pietro
Published: January 2021

Ultraviolet (UV) is a component of solar radiation that can be divided into three types defined by waveband: UV-A (315–400 nm), UV-B (280–315 nm), and UV-C (<280 nm). UV light can influence the physiological responses of plants. Wavelength, intensity, and exposure have a great impact on plant growth and quality. Interaction between plants and UV light is regulated by photoreceptors such as UV Resistance Locus 8 (UVR8) that enables acclimation to UV-B stress. Although UV in high doses is known to damage quality and production parameters, some studies show that UV in low doses may stimulate biomass accumulation and the synthesis of healthy compounds that mainly absorb UV. UV exposure is known to induce variations in plant architecture, important in ornamental crops, increasing their economic value. Abiotic stress induced by UV exposure increases resistance to insects and pathogens, and reduce postharvest quality depletion. This review highlights the role that UV may play in plant growth, quality, photomorphogenesis, and abiotic/biotic stress resistance.
Intimate Partner Violence against Women with Disabilities in Spain: A Public Health Problem
Authors: Meseguer-Santamaría María-Leticia , Sánchez-Alberola Francisco , Vargas-Vargas Manuel
Published: January 2021

Violence against women with disabilities is a social problem with important consequences for their physical and mental health. The World Health Organization (WHO) declared violence against women as a public health priority issue in 1996 and the fact that violence is used by the intimate partner and upon women with disabilities exacerbates the situation. Therefore, this is an issue that must be addressed from a public health viewpoint. Violence is studied from various aspects: Physical, psychological, sexual, or social control, and its multiple consequences in women’s health and the use of health services. In this perspective, with the data from the VI Violence against Women Macro-survey 2019 (VWM-2019) and adjusted to Spain, this study examines the incidence of intimate partner violence and its consequences in the health of women with disabilities and its impact on health services. Using binary logistic regression, the greater vulnerability of this group to these attacks is stated and the need to address this issue to improve the health of these people is brought to light.
Degree of Impact of Tailor’s Bunion on Quality of Life: A Case–Control Study
Authors: Mazoteras-Pardo Victoria , Becerro-de-Bengoa-Vallejo Ricardo , Losa-Iglesias Marta , Palomo-López Patricia , López-López Daniel , Calvo-Lobo César , Romero-Morales Carlos , Casado-Hernández Israel
Published: January 2021

Tailor’s bunion (TB) disease should be considered one of the foot injuries that causes disability in feet as well as general health. This case–control descriptive study investigated and contrasted the effects of different TB types in a sociodemographic population using the Foot Health Status Questionnaire (FHSQ). A sample of 100 subjects with a mean age of 51.70 ± 17.78 years was recruited and requested to reply to a foot health survey. Results were self-reported. Subjects were scored. Participants with TB type III (TB3) registered lower scores for foot pain, foot function, footwear, and foot health. Physical activity and social capacity had higher scores, and vigor and general health were lower. A Kruskal–Wallis test was used for systematic differences between the FHSQ and different TB types. In all analyses, statistical significance was considered a p-value <0.05 with a 95% confidence interval. Statistically significant differences were found between all domains of the FHSQ and TB, except for the social capacity domain and vigor. The FHSQ is an important measurement tool in TB subjects, showing that factors such as sex, age, and footwear used throughout an individual’s life are significantly associated with the development of TB3 and its influence on foot pain and foot health.
COVID-19, Fake News, and Vaccines: Should Regulation Be Implemented?
Authors: Marco-Franco Julio Emilio , Pita-Barros Pedro , Vivas-Orts David , González-de-Julián Silvia , Vivas-Consuelo David
Published: January 2021

We analysed issues concerning the establishment of compulsory vaccination against COVID-19, as well as the role of misinformation as a disincentive—especially when published by health professionals—and citizen acceptance of measures in this regard. Data from different surveys revealed a high degree of hesitation rather than outright opposition to vaccines. The most frequent complaint related to the COVID-19 vaccination was the fear of side effects. Within the Spanish and European legislative framework, both compulsory vaccination and government regulation of FN (Fake News) appear to be feasible options, counting on sufficient legal support, which could be reinforced by additional amendment. However, following current trends of good governance, policymakers must have public legitimation. Rather than compulsory COVID-19 vaccination, an approach based on education and truthful information, persuading the population of the benefits of a vaccine on a voluntary basis, is recommended. Disagreements between health professionals are positive, but they should be resolved following good practice and the procedures of the code of ethics. Furthermore, citizens do not support the involvement of government authorities in the direct control of news. Collaboration with the media and other organizations should be used instead.
Exploring the Relationship between Rising Temperatures and the Number of Climate-Related Natural Disasters in China
Authors: Zhu Mingan , Fan Bihang
Published: January 2021

Warming has strongly influenced the quantity and variability of natural disasters around the globe. This study aims to characterize the varying patterns between rising temperatures and climate-related natural disasters in China from 1951 to 2010. We examined the overall trend in the patterns of an 11-year cycle, and climate-related natural disaster responses to periods of rising and dropping temperature. We used Morlet wavelet analysis to determine the length of a temperature cycle period, and the arc elasticity coefficient to assess the number of climate-related natural disasters in response to the changing temperature. We found that: (1) the overall relationship between temperature and the number of climate-related natural disasters was positive; (2) however, on the cycle level, the pattern of climate-related natural disasters was found to be independent of temperature variation; (3) on the rise-drop level, temperature increases were associated with declines in the number of climate-related natural disasters. Moreover, as temperature decreased, the number of climate-related natural disasters increased substantially, such that temperature had a more considerable influence on the quantity of climate-related natural disasters during the temperature-drop period. Findings in this study can help enhance the dissemination of warning and mitigation efforts to combat natural disasters in the changing climate.
Psychosocial Factors Predicting Resilience in Family Caregivers of Children with Cancer: A Cross-Sectional Study
Authors: Toledano-Toledano Filiberto , Luna David , Moral de la Rubia José , Martínez Valverde Silvia , Bermúdez Morón Carlos Alberto , Salazar García Marcela , Vasquez Pauca Mario José
Published: January 2021

Chronic diseases in childhood can affect the physical and mental health of patients and their families. The objective of this study was to identify the sociodemographic and psychosocial factors that predict resilience in family caregivers of children with cancer and to define whether there are differences in the levels of resilience derived from these sociodemographic variables. Three hundred and thirty family caregivers of children with cancer, with an average age of 32.6 years were interviewed. The caregivers responded to a battery of tests that included a questionnaire of sociodemographic variables, the Measuring Scale of Resilience, the Beck Depression Inventory, the Inventory of Quality of Life, the Beck Anxiety Inventory, an interview of caregiver burden and the World Health Organization Well-Being Index. The main findings indicate that family caregivers of children with cancer reported high levels of resilience, which were associated positively with quality of life, psychological well-being and years of study and associated negatively with depression, anxiety and caregiver burden. The variables that predicted resilience in families of children with cancer were quality of life, psychological well-being, depression and number of children. Family caregivers who were married and Catholic showed higher resilience scores. We conclude that being a caregiver in a family with children with cancer is associated with symptoms of anxiety and with depressive episodes. These issues can be overcome through family strength, well-being, quality of life and positive adaptation processes and mobilization of family resources.
All That Glitters Is Not Silver—A New Look at Microbiological and Medical Applications of Silver Nanoparticles
Authors: Kowalczyk Paweł , Szymczak Mateusz , Maciejewska Magdalena , Laskowski Łukasz , Laskowska Magdalena , Ostaszewski Ryszard , Skiba Grzegorz , Franiak-Pietryga Ida
Published: January 2021

Silver and its nanoparticles (AgNPs) have different faces, providing different applications. In recent years, the number of positive nanosilver applications has increased substantially. It has been proven that AgNPs inhibit the growth and survival of bacteria, including human and animal pathogens, as well as fungi, protozoa and arthropods. Silver nanoparticles are known from their antiviral and anti-cancer properties; however, they are also very popular in medical and pharmaceutical nanoengineering as carriers for precise delivery of therapeutic compounds, in the diagnostics of different diseases and in optics and chemistry, where they act as sensors, conductors and substrates for various syntheses. The activity of AgNPs has not been fully discovered; therefore, we need interdisciplinary research to fulfil this knowledge. New forms of products with silver will certainly find application in the future treatment of many complicated and difficult to treat diseases. There is still a lack of appropriate and precise legal condition regarding the circulation of nanomaterials and the rules governing their safety use. The relatively low toxicity, relative biocompatibility and selectivity of nanoparticle interaction combined with the unusual biological properties allow their use in animal production as well as in bioengineering and medicine. Despite a quite big knowledge on this topic, there is still a need to organize the data on AgNPs in relation to specific microorganisms such as bacteria, viruses or fungi. We decided to put this knowledge together and try to show positive and negative effects on prokaryotic and eukaryotic cells.
Molecular Aspects of Pruritus Pathogenesis in Psoriasis
Authors: Jaworecka Kamila , Muda-Urban Joanna , Rzepko Marian , Reich Adam
Published: January 2021

Psoriasis is a chronic, systemic inflammatory disease with a genetic background that involves almost 3% of the general population worldwide. Approximately, 70–90% of patients with psoriasis suffer from pruritus, an unpleasant sensation that provokes a desire to scratch. Despite the enormous progress in understanding the mechanisms that cause psoriasis, the pathogenesis of psoriasis-related pruritus still remains unclear. In order to improve patients’ quality of life, development of more effective and safer antipruritic therapies is necessary. In turn to make it possible, better understanding of complexed and multifactorial pathogenesis of this symptom is needed. In this article we have systematized the current knowledge about pruritus origin in psoriasis.
BAFF Inhibition Effectively Suppresses the Development of Anti-HLA.A2 Antibody in the Highly Sensitized Mouse Model
Authors: Min Ji Won , Shin Yoo-Jin , Lee Hyeyoung , Kim Bo-Mi , Park Ki Hyun , Doh Kyoung Chan , Kim Tae-Min , Lim Sun Woo , Yang Chul Woo , Oh Eun-Jee , Chung Byung Ha
Published: January 2021

B cell activating factor (BAFF) is a cytokine that plays a role in the survival, proliferation and differentiation of B cells. We proposed to observe the effects of BAFF inhibition on the humoral immune responses of an allosensitized mouse model using HLA.A2 transgenic mice. Wild-type C57BL/6 mice were sensitized with skin allografts from C57BL/6-Tg (HLA-A2.1)1Enge/J mice and were treated with anti-BAFF monoclonal antibody (mAb) (named Sandy-2) or control IgG1 antibody. HLA.A2-specific IgG was reduced in BAFF-inhibited mice compared to the control group (Δ-13.62 vs. Δ27.07, p < 0.05). BAFF inhibition also resulted in increased pre-pro and immature B cell proportions and decreased mature B cells in the bone marrow (p < 0.05 vs. control). In the spleen, an increase in transitional B cells was observed with a significant decrease in marginal and follicular B cells (p < 0.05 vs. control). There was no significant difference in the proportions of long-lived plasma and memory B cells. Microarray analysis showed that 19 gene probes were significantly up- (>2-fold, p < 0.05) or down-regulated (≤2-fold, p < 0.05) in the BAFF-inhibited group. BAFF inhibition successfully reduced alloimmune responses through the reduction in alloantibody production and suppression of B cell differentiation and maturation. Our data suggest that BAFF suppression may serve as a useful target in desensitization therapy.
Survival Pathways Are Differently Affected by Microgravity in Normal and Cancerous Breast Cells
Authors: Monti Noemi , Masiello Maria Grazia , Proietti Sara , Catizone Angela , Ricci Giulia , Harrath Abdel Halim , Alwasel Saleh H. , Cucina Alessandra , Bizzarri Mariano
Published: January 2021

Metazoan living cells exposed to microgravity undergo dramatic changes in morphological and biological properties, which ultimately lead to apoptosis and phenotype reprogramming. However, apoptosis can occur at very different rates depending on the experimental model, and in some cases, cells seem to be paradoxically protected from programmed cell death during weightlessness. These controversial results can be explained by considering the notion that the behavior of adherent cells dramatically diverges in respect to that of detached cells, organized into organoids-like, floating structures. We investigated both normal (MCF10A) and cancerous (MCF-7) breast cells and found that appreciable apoptosis occurs only after 72 h in MCF-7 cells growing in organoid-like structures, in which major modifications of cytoskeleton components were observed. Indeed, preserving cell attachment to the substrate allows cells to upregulate distinct Akt- and ERK-dependent pathways in MCF-7 and MCF-10A cells, respectively. These findings show that survival strategies may differ between cell types but cannot provide sufficient protection against weightlessness-induced apoptosis alone if adhesion to the substrate is perturbed.
Diverse Evolution in 111 Plant Genomes Reveals Purifying and Dosage Balancing Selection Models for F-Box Genes
Authors: Hua Zhihua
Published: January 2021

The F-box proteins function as substrate receptors to determine the specificity of Skp1-Cul1-F-box ubiquitin ligases. Genomic studies revealed large and diverse sizes of the F-box gene superfamily across plant species. Our previous studies suggested that the plant F-box gene superfamily is under genomic drift evolution promoted by epigenomic programming. However, how the size of the superfamily drifts across plant genomes is currently unknown. Through a large-scale genomic and phylogenetic comparison of the F-box gene superfamily covering 110 green plants and one red algal species, I discovered four distinct groups of plant F-box genes with diverse evolutionary processes. While the members in Clusters 1 and 2 are species/lineage-specific, those in Clusters 3 and 4 are present in over 46 plant genomes. Statistical modeling suggests that F-box genes from the former two groups are skewed toward fewer species and more paralogs compared to those of the latter two groups whose presence frequency and sizes in plant genomes follow a random statistical model. The enrichment of known Arabidopsis F-box genes in Clusters 3 and 4, along with comprehensive biochemical evidence showing that Arabidopsis members in Cluster 4 interact with the Arabidopsis Skp1-like 1 (ASK1), demonstrates over-representation of active F-box genes in these two groups. Collectively, I propose purifying and dosage balancing selection models to explain the lineage/species-specific duplications and expansions of F-box genes in plant genomes. The purifying selection model suggests that most, if not all, lineage/species-specific F-box genes are detrimental and are thus kept at low frequencies in plant genomes.
Structure, Activation and Regulation of NLRP3 and AIM2 Inflammasomes
Authors: Sharma Meenakshi , de Alba Eva
Published: January 2021

The inflammasome is a three-component (sensor, adaptor, and effector) filamentous signaling platform that shields from multiple pathogenic infections by stimulating the proteolytical maturation of proinflammatory cytokines and pyroptotic cell death. The signaling process initiates with the detection of endogenous and/or external danger signals by specific sensors, followed by the nucleation and polymerization from sensor to downstream adaptor and then to the effector, caspase-1. Aberrant activation of inflammasomes promotes autoinflammatory diseases, cancer, neurodegeneration, and cardiometabolic disorders. Therefore, an equitable level of regulation is required to maintain the equilibrium between inflammasome activation and inhibition. Recent advancement in the structural and mechanistic understanding of inflammasome assembly potentiates the emergence of novel therapeutics against inflammasome-regulated diseases. In this review, we have comprehensively discussed the recent and updated insights into the structure of inflammasome components, their activation, interaction, mechanism of regulation, and finally, the formation of densely packed filamentous inflammasome complex that exists as micron-sized punctum in the cells and mediates the immune responses.
Charge Mobility in Discotic Liquid Crystals
Authors: Termine Roberto , Golemme Attilio
Published: January 2021

Discotic (disk-shaped) molecules or molecular aggregates may form, within a certain temperature range, partially ordered phases, known as discotic liquid crystals, which have been extensively studied in the recent past. On the one hand, this interest was prompted by the fact that they represent models for testing energy and charge transport theories in organic materials. However, their long-range self-assembling properties, potential low cost, ease of processability with a variety of solvents and the relative ease of tailoring their properties via chemical synthesis, drove the attention of researchers also towards the exploitation of their semiconducting properties in organic electronic devices. This review covers recent research on the charge transport properties of discotic mesophases, starting with an introduction to their phase structure, followed by an overview of the models used to describe charge mobility in organic substances in general and in these systems in particular, and by the description of the techniques most commonly used to measure their charge mobility. The reader already familiar or not interested in such details can easily skip these sections and refer to the core section of this work, focusing on the most recent and significant results regarding charge mobility in discotic liquid crystals.
De Novo Transcriptome Sequencing of Rough Lemon Leaves (Citrus jambhiri Lush.) in Response to Plenodomus tracheiphilus Infection
Authors: Russo Riccardo , Sicilia Angelo , Caruso Marco , Arlotta Carmen , Di Silvestro Silvia , Gmitter Frederick G. , Nicolosi Elisabetta , Lo Piero Angela Roberta
Published: January 2021

Mal secco is one of the most severe diseases of citrus, caused by the necrotrophic fungus Plenodomus tracheiphilus. With the main aim of identifying candidate genes involved in the response of citrus plants to “Mal secco”, we performed a de novo transcriptome analysis of rough lemon seedlings subjected to inoculation of P. tracheiphilus. The analysis of differential expressed genes (DEGs) highlighted a sharp response triggered by the pathogen as a total of 4986 significant DEGs (2865 genes up-regulated and 2121 down-regulated) have been revealed. The analysis of the most significantly enriched KEGG pathways indicated that a crucial role is played by genes involved in “Plant hormone signal transduction”, “Phenylpropanoid biosynthesis”, and “Carbon metabolism”. The main findings of this work are that under fungus challenge, the rough lemon genes involved both in the light harvesting and the photosynthetic electron flow were significantly down-regulated, thus probably inducing a shortage of energy for cellular functions. Moreover, the systemic acquired resistance (SAR) was activated through the induced salicylic acid cascade. Interestingly, RPM1 interacting protein 4, an essential positive regulator of plant defense, and BIR2, which is a negative regulator of basal level of immunity, have been identified thus representing useful targets for molecular breeding.
PEG-BHD1028 Peptide Regulates Insulin Resistance and Fatty Acid β-Oxidation, and Mitochondrial Biogenesis by Binding to Two Heterogeneous Binding Sites of Adiponectin Receptors, AdipoR1 and AdipoR2
Authors: Lee In Kyung , Kim Gyuyoup , Kim Do-Hwi , Kim Brian B.
Published: January 2021

Adiponectin plays multiple critical roles in modulating various physiological processes by binding to its receptors. The functions of PEG-BHD1028, a potent novel peptide agonist to AdipoRs, was evaluated using in vitro and in vivo models based on the reported action spectrum of adiponectin. To confirm the design concept of PEG-BHD1028, the binding sites and their affinities were analyzed using the SPR (Surface Plasmon Resonance) assay. The results revealed that PEG-BHD1028 was bound to two heterogeneous binding sites of AdipoR1 and AdipoR2 with a relatively high affinity. In C2C12 cells, PEG-BHD1028 significantly activated AMPK and subsequent pathways and enhanced fatty acid β-oxidation and mitochondrial biogenesis. Furthermore, it also facilitated glucose uptake by lowering insulin resistance in insulin-resistant C2C12 cells. PEG-BHD1028 significantly reduced the fasting plasma glucose level in db/db mice following a single s.c. injection of 50, 100, and 200 μg/Kg and glucose tolerance at a dose of 50 μg/Kg with significantly decreased insulin production. The animals received 5, 25, and 50 μg/Kg of PEG-BHD1028 for 21 days significantly lost their weight after 18 days in a range of 5–7%. These results imply the development of PEG-BHD1028 as a potential adiponectin replacement therapeutic agent.