bluuebunny/crossref_metadata_2025 huggingface dataset:
COVID-19: A Recommendation to Examine the Effect of Mouthrinses with β-Cyclodextrin Combined with Citrox in Preventing Infection and Progression
Authors: Carrouel Florence , Conte Maria Pia , Fisher Julian , Gonçalves Lucio Souza , Dussart Claude , Llodra Juan Carlos , Bourgeois Denis
Published: April 2020

Considered to be a major portal of entry for infectious agents, the oral cavity is directly associated with the evolutionary process of SARS-CoV-2 in its inhalation of ambient particles in the air and in expectorations. Some new generations of mouth rinses currently on the market have ingredients that could contribute to lower the SARS-CoV-2 viral load, and thus facilitate the fight against oral transmission. If chlorhexidine, a usual component of mouth rinse, is not efficient to kill SARS-CoV-2, the use of a mouth rinses and/or with local nasal applications that contain β-cyclodextrins combined with flavonoids agents, such as Citrox, could provide valuable adjunctive treatment to reduce the viral load of saliva and nasopharyngeal microbiota, including potential SARS-CoV-2 carriage. We urge national agencies and authorities to start clinical trials to evaluate the preventive effects of βCD-Citrox therapeutic oral biofilm rinses in reducing the viral load of the infection and possibly disease progression.
Electronic Tuning Square-Wave Generators with Improved Linearity Using Operational Transresistance Amplifier
Authors: Chandra Shaker Pittala , Srinivasulu Avireni
Published: April 2020

Two new electronic tuning current-mode square-wave generators are introduced in the ensuing paper. In the first proposed square-wave generator circuit, one Operational Trans-resistance Amplifier (OTRA) and two passive components are involved, along with two NMOS depletion mode transistors. This circuit generates a square-wave with almost equal and fixed duty cycles. The second proposed circuit is able to control both on-duty and off-duty cycles independently with the help of two passive components, two NMOS depletion mode transistors, and two diodes connected to the circuit. The frequency of the proposed circuits can be adjusted with the passive components connected to the circuit. Moreover, electronic tuning can also be achieved with the proposed circuits. The measured results that are included in the paper show the linear variation of a time period as compared with existing OTRA based square waveform generator. The performance of the proposed circuits is examined while using SPICE models. These circuits are built on a laboratory breadboard using commercially available Current Feedback Operational Amplifier (AD844 AN) and passive components are connected externally and tested for square waveform generation. The obtained results demonstrate good agreement with the theoretical values.
Design and Performance of Permeable Groins on a Low-Energy Natural Beach
Authors: Torres-Freyermuth Alec , Hofman Anne , Tuz-Pech José Clemente , Medellín Gabriela , Roos Pieter C.
Published: April 2020

Beach erosion, ubiquitous of sandy coasts around the world, can be exacerbated or mitigated with the use of hard engineering solutions including groins. The use of groins has been discouraged in many countries despite its capability to reduce sand losses. This study aims to design a 15 m permeable groin that allows sediment bypassing. Field experiments were conducted on a low-energy, sea-breeze dominated micro-tidal beach at the northern Yucatan peninsula during both mean and extreme wave conditions. Firstly, a short-term experiment consisted in monitoring the structure performance for 24 h during typical sea breeze conditions and the subsequent beach recovery after the structure removal. Secondly, a multi-day (60 days) experiment was conducted to investigate the performance of a single- and double-groin system during both mean and local storm conditions. Beach surveys were conducted to evaluate the beach response. Shoreline variability shows sediment accretion on the east side of the structure during sea breezes, whereas during NNW storm events, the accretion occurred on the west side. Importantly, on a scale of days, sediment bypassing occurs, and hence a net accretion was measured at both sides of the structure. During this time, a second structure was deployed 30 m apart to test the performance of a double-groin system, finding a net shoreline advance at all beach transects. Thus, low-crested short-length permeable groins are found to be a suitable emergency mitigation measure against beach erosion in micro-tidal environments causing moderate shoreline change.
Computation of Analytical Zoom Locus Using Padé Approximation
Authors: Kim Kang Min , Choe Sun-Ho , Ryu Jae-Myung , Choi Hojong
Published: April 2020

When the number of lens groups is large, the zoom locus becomes complicated and thus cannot be determined by analytical means. By the conventional calculation method, it is possible to calculate the zoom locus only when a specific lens group is fixed or the number of lens groups is small. To solve this problem, we employed the Padé approximation to find the locus of each group of zoom lenses as an analytic form of a rational function consisting of the ratio of polynomials, programmed in MATLAB. The Padé approximation is obtained from the initial data of the locus of each lens group. Subsequently, we verify that the obtained locus of lens groups satisfies the effective focal length (EFL) and the back focal length (BFL). Afterwards, the Padé approximation was applied again to confirm that the error of BFL is within the depth of focus for all zoom positions. In this way, the zoom locus for each lens group of the optical system with many moving lens groups was obtained as an analytical rational function. The practicality of this method was verified by application to a complicated zoom lens system with five or more lens groups using preset patents.
Photo-Thermal Interactions in a Semiconducting Media with a Spherical Cavity under Hyperbolic Two-Temperature Model
Authors: Alzahrani Faris S. , Abbas Ibrahim A.
Published: April 2020

This article highlights the study of photo-thermoelastic interaction in an unbounded semiconductor medium containing a spherical cavity. This problem is solved using the new hyperbolic two-temperature model. The bounding surface of the cavity is traction free and loaded thermally by exponentially decaying pulse boundary heat flux. In addition, the carrier density is prescribed on the inner surface of the cavity in terms of the recombination speed. The techniques of Laplace transforms are used to get the analytical solutions of the problem in the transformed domain by the eigenvalues method. The inversions of Laplace transformations have been carried out numerically. The outcomes also display that the analytical schemes can overcome the mathematical problem to analyze this problem. Numerical outcomes for a semiconductor material are performed and demonstrated graphically. According to the numerical results, this new hyperbolic two-temperature model of thermoelasticity offers finite speed of the thermal wave and mechanical wave propagation.
Powers of the Stochastic Gompertz and Lognormal Diffusion Processes, Statistical Inference and Simulation
Authors: Ramos-Ábalos Eva María , Gutiérrez-Sánchez Ramón , Nafidi Ahmed
Published: April 2020

In this paper, we study a new family of Gompertz processes, defined by the power of the homogeneous Gompertz diffusion process, which we term the powers of the stochastic Gompertz diffusion process. First, we show that this homogenous Gompertz diffusion process is stable, by power transformation, and determine the probabilistic characteristics of the process, i.e., its analytic expression, the transition probability density function and the trend functions. We then study the statistical inference in this process. The parameters present in the model are studied by using the maximum likelihood estimation method, based on discrete sampling, thus obtaining the expression of the likelihood estimators and their ergodic properties. We then obtain the power process of the stochastic lognormal diffusion as the limit of the Gompertz process being studied and go on to obtain all the probabilistic characteristics and the statistical inference. Finally, the proposed model is applied to simulated data.
Plasma 25-Hydroxyvitamin D Concentrations are Associated with Polyunsaturated Fatty Acid Metabolites in Young Children: Results from the Vitamin D Antenatal Asthma Reduction Trial
Authors: Huang Mengna , Kelly Rachel S. , Kachroo Priyadarshini , Chu Su H. , Lee-Sarwar Kathleen , Chawes Bo L. , Bisgaard Hans , Litonjua Augusto A. , Weiss Scott T. , Lasky-Su Jessica
Published: April 2020

Vitamin D deficiency contributes to a multitude of health conditions, but its biological mechanisms are not adequately understood. Untargeted metabolomics offers the opportunity to comprehensively examine the metabolic profile associated with variations in vitamin D concentrations. The objective of the current analysis was to identify metabolites and metabolic pathways associated with plasma 25-hydroxyvitamin D [25(OH)D] concentrations. The current study included children of pregnant women in the Vitamin D Antenatal Asthma Reduction Trial, who had 25(OH)D and global metabolomics data at age 1 and 3 years. We assessed the cross-sectional associations between individual metabolites and 25(OH)D using linear regression adjusting for confounding factors. Twelve metabolites were significantly associated with plasma 25(OH)D concentrations at both age 1 and 3 after correction for multiple comparisons, including three members of the n-6 polyunsaturated fatty acid (PUFA) metabolism pathway (linoleate, arachidonate, and docosapentaenoate) inversely associated with 25(OH)D. These PUFAs along with four other significant metabolites were replicated in the independent Childhood Asthma Management Program (CAMP) cohort. Both vitamin D and n-6 PUFAs are involved in inflammatory processes, and evidence from cell and animal studies demonstrate a plausible biological mechanism where the active form of 25(OH)D may influence n-6 PUFA metabolism. These relationships warrant further investigation in other populations.
Network Analysis Provides Insight into Tomato Lipid Metabolism
Authors: Kuhalskaya Anastasiya , Wijesingha Ahchige Micha , Perez de Souza Leonardo , Vallarino José , Brotman Yariv , Alseekh Saleh
Published: April 2020

Metabolic correlation networks have been used in several instances to obtain a deeper insight into the complexity of plant metabolism as a whole. In tomato (Solanum lycopersicum), metabolites have a major influence on taste and overall fruit quality traits. Previously a broad spectrum of metabolic and phenotypic traits has been described using a Solanum pennellii introgression-lines (ILs) population. To obtain insights into tomato fruit metabolism, we performed metabolic network analysis from existing data, covering a wide range of metabolic traits, including lipophilic and volatile compounds, for the first time. We provide a comprehensive fruit correlation network and show how primary, secondary, lipophilic, and volatile compounds connect to each other and how the individual metabolic classes are linked to yield-related phenotypic traits. Results revealed a high connectivity within and between different classes of lipophilic compounds, as well as between lipophilic and secondary metabolites. We focused on lipid metabolism and generated a gene-expression network with lipophilic metabolites to identify new putative lipid-related genes. Metabolite–transcript correlation analysis revealed key putative genes involved in lipid biosynthesis pathways. The overall results will help to deepen our understanding of tomato metabolism and provide candidate genes for transgenic approaches toward improving nutritional qualities in tomato.
Breakup Dynamics of Semi-dilute Polymer Solutions in a Microfluidic Flow-focusing Device
Authors: Xue Chun-Dong , Chen Xiao-Dong , Li Yong-Jiang , Hu Guo-Qing , Cao Tun , Qin Kai-Rong
Published: April 2020

Droplet microfluidics involving non-Newtonian fluids is of great importance in both fundamental mechanisms and practical applications. In the present study, breakup dynamics in droplet generation of semi-dilute polymer solutions in a microfluidic flow-focusing device were experimentally investigated. We found that the filament thinning experiences a transition from a flow-driven to a capillary-driven regime, analogous to that of purely elastic fluids, while the highly elevated viscosity and complex network structures in the semi-dilute polymer solutions induce the breakup stages with a smaller power-law exponent and extensional relaxation time. It is elucidated that the elevated viscosity of the semi-dilute solution decelerates filament thinning in the flow-driven regime and the incomplete stretch of polymer molecules results in the smaller extensional relaxation time in the capillary-driven regime. These results extend the understanding of breakup dynamics in droplet generation of non-Newtonian fluids and provide guidance for microfluidic synthesis applications involving dense polymeric fluids.
Electrochemical Biosensors for Determination of Colorectal Tumor Biomarkers
Authors: Quinchia Jennifer , Echeverri Danilo , Cruz-Pacheco Andrés , Maldonado María , Orozco Jahir
Published: April 2020

The accurate determination of specific tumor markers associated with cancer with non-invasive or minimally invasive procedures is the most promising approach to improve the long-term survival of cancer patients and fight against the high incidence and mortality of this disease. Quantification of biomarkers at different stages of the disease can lead to an appropriate and instantaneous therapeutic action. In this context, the determination of biomarkers by electrochemical biosensors is at the forefront of cancer diagnosis research because of their unique features such as their versatility, fast response, accurate quantification, and amenability for multiplexing and miniaturization. In this review, after briefly discussing the relevant aspects and current challenges in the determination of colorectal tumor markers, it will critically summarize the development of electrochemical biosensors to date to this aim, highlighting the enormous potential of these devices to be incorporated into the clinical practice. Finally, it will focus on the remaining challenges and opportunities to bring electrochemical biosensors to the point-of-care testing.
Knockdown of Dinoflagellate Condensin CcSMC4 Subunit Leads to S-Phase Impediment and Decompaction of Liquid Crystalline Chromosomes
Authors: Yan Ting Hin Kosmo , Wu Zhihao , Kwok Alvin Chun Man , Wong Joseph Tin Yum
Published: April 2020

Dinoflagellates have some of the largest genomes, and their liquid-crystalline chromosomes (LCCs) have high degrees of non-nucleosomal superhelicity with cation-mediated DNA condensation. It is currently unknown if condensins, pentameric protein complexes containing structural maintenance of chromosomes 2/4, commonly involved in eukaryotic chromosomes condensation in preparation for M phase, may be involved in the LCC structure. We find that CcSMC4p (dinoflagellate SMC4 homolog) level peaked at S/G2 phase, even though LCCs do not undergo global-decondensation for replication. Despite the differences in the chromosomal packaging system, heterologous CcSMC4p expression suppressed conditional lethality of the corresponding fission yeast mutant, suggesting conservation of some canonical condensin functions. CcSMC4p-knockdown led to sustained expression of the S-phase marker PCNAp, S-phase impediment, and distorted nuclei in the early stage of CcSMC4p depletion. Prolonged CcSMC4p-knockdown resulted in aneuploidal cells and nuclear swelling with increasing LCC decompaction–decondensation. Cumulatively, our data suggested CcSMC4p function was required for dinoflagellate S-phase progression, and we propose that condensin-mediated higher-order compaction provisioning is involved in the provision of local rigidity for the replisome.
Antioxidative Activity of 1,3,5-Triazine Analogues Incorporating Aminobenzene Sulfonamide, Aminoalcohol/Phenol, Piperazine, Chalcone, or Stilbene Motifs
Authors: Havránková Eva , Čalkovská Nikola , Padrtová Tereza , Csöllei Jozef , Opatřilová Radka , Pazdera Pavel
Published: April 2020

A series of 1,3,5-triazine analogues, incorporating aminobenzene sulfonamide, aminoalcohol/phenol, piperazine, chalcone, or stilbene structural motifs, were evaluated as potential antioxidants. The compounds were prepared by using step-by-step nucleophilic substitution of chlorine atoms in starting 2,4,6-trichloro-1,3,5-triazine. Reactions were catalyzed by Cu(I)-supported on a weakly acidic resin. The radical scavenging activity was determined in terms of %inhibition activity and EC50, using the ABTS method. Trolox and ascorbic acid (ASA) were used as standards. In the lowest concentration 1 × 10−4 M, the %inhibition activity values at 0 min were comparable with both standards at least for 10 compounds. After 60 min, compounds 5, 6, 13, and 25 showed nearly twice %inhibition (73.44–87.09%) in comparison with the standards (Trolox = 41.49%; ASA = 31.07%). Values of EC50 at 60 min (17.16–27.78 μM) were 5 times lower for compounds 5, 6, 13, and 25 than EC50 of both standards (trolox = 178.33 μM; ASA = 147.47 μM). Values of EC50 correlated with %inhibition activity. Based on these results, the presented 1,3,5-triazine analogues have a high potential in the treatment of illnesses caused or related to oxidative stress.
Separation of Biological Entities from Human Blood by Using Magnetic Nanocomposites Obtained from Zeolite Precursors
Authors: Esposito Serena , Marocco Antonello , Dell’Agli Gianfranco , Bonelli Barbara , Mannu Franca , Allia Paolo , Tiberto Paola , Barrera Gabriele , Pansini Michele
Published: April 2020

In this work, three novel magnetic metal–ceramic nanocomposites were obtained by thermally treating Fe-exchanged zeolites (either A or X) under reducing atmosphere at relatively mild temperatures (750–800 °C). The so-obtained materials were thoroughly characterized from the point of view of their physico-chemical properties and, then, used as magnetic adsorbents in the separation of the target gene factors V and RNASE and of the Staphylococcus aureus bacteria DNA from human blood. Such results were compared with those obtained by using a top ranking commercial separation system (namely, SiMAG-N-DNA by Chemicell). The results obtained by using the novel magnetic adsorbents were similar to (or even better than) those obtained by using the commercial system, both during manual and automated separations, provided that a proper protocol was adopted. Particularly, the novel magnetic adsorbents showed high sensitivity during tests performed with small volumes of blood. Finally, the feasible production of such magnetic adsorbents by an industrial process was envisaged as well.
Characterization of Free and Bound Phenolic Acids and Flavonoid Aglycones in Rosa rugosa Thunb. Leaves and Achenes Using LC–ESI–MS/MS–MRM Methods
Authors: Olech Marta , Pietrzak Wioleta , Nowak Renata
Published: April 2020

Fast and reliable determination of polyphenols is a quite common goal during investigation of new plant materials and herbal products, their standardization, quality control, or chemo-taxonomical studies. The aim of this study was to develop and validate methods based on the application of reversed phase liquid chromatography/electrospray ionization triple quadrupole mass spectrometry (LC-ESI-MS/MS) using multiple reaction monitoring (MRM) for comprehensive quantitative and qualitative analysis of phenolic acids and flavonoid aglycones. LC-MS/MS-MRM protocols were applied for the determination of free and bound phenolics in a series of plant samples prepared from leaves and achenes (true fruits) of Japanese rose (Rosa rugosa Thunb.). The presence of large amount of phenolic compounds was detected in rose leaves (786.44 µg/g and 14.46 µg/g of phenolic acids and flavonoid aglycones, respectively). Isoferulic acid and five aglycones were revealed for the first time in this plant material. Moreover, 15 phenolic acids and six aglycones were found in the rose achenes, including eight phenolic acids and four aglycones that had not been previously reported in this rose organ. It was shown that leaves and achenes may constitute an industrially relevant source of phenolic compounds for potential commercial use in pharmaceutical, food, or cosmetic industry.
Structural Elucidation of Malonylcommunol and 6β-Hydroxy-trans-communic Acid, Two Undescribed Diterpenes from Salvia cinnabarina. First Examples of Labdane Diterpenoids from a Mexican Salvia Species
Authors: Bustos-Brito Celia , Nieto-Camacho Antonio , Hernandez-Ortega Simón , Rivera-Chávez José , Quijano Leovigildo , Esquivel Baldomero
Published: April 2020

The aerial parts of Salvia cinnabarina afforded two undescribed labdane diterpenoids 1 and 2 (malonylcommunol and 6β-hydroxy-trans-communic acid) along with two known labdane diterpenoids, trans-communic acid (3) and trans-communol (4). Additionally, seven known metabolites were also isolated; two isopimarane diterpenoids 5 and 6, two sesquiterpenoids identified as β-eudesmol (7) and cryptomeridiol (8), and three aromatic compounds identified as phthalic acid (9), a mixture of tyrosol fatty acid esters (10) and the flavone salvigenine (11). While compounds compounds 1–3 showed significant inhibition of yeast α-glucosidase, compounds 2, 3 and 7 had no anti-inflammatory activity in the edema model induced by TPA. This paper is not only the first report on a wild population of Salvia cinnabarina, but also of the presence of labdane-type diterpenoids in a Mexican Salvia sp.
Thermodynamic, Physical, and Structural Characteristics in Layered Hybrid Type (C2H5NH3)2MCl4 (M = 59Co, 63Cu, 65Zn, and 113Cd) Crystals
Authors: Lim Ae Ran
Published: April 2020

The thermal, physical, and molecular dynamics of layered hybrid type (C2H5NH3)2MCl4 (M = 59Co, 63Cu, 65Zn, and 113Cd) crystals were investigated by thermogravimetric analysis (TGA) and magic angle spinning nuclear magnetic resonance (MAS NMR) spectroscopy. The temperatures of the onset of partial thermal decomposition were found to depend on the identity of M. In addition, the Bloembergen–Purcell–Pound curves for the 1H spin-lattice relaxation time T1ρ in the rotating frames of CH3CH2 and NH3, and for the 13C T1ρ of CH3 and CH2 were shown to exhibit minima as a function of the inverse temperature. These results confirmed the rotational motion of 1H and 13C in the C2H5NH3 cation. Finally, the T1ρ values and activation energies Ea obtained from the 1H measurements for the H‒Cl···M (M = Zn and Cd) bond in the absence of paramagnetic ions were larger than those obtained for the H‒Cl···M (M = Co and Cu) bond in the presence of paramagnetic ions. Moreover, the Ea value for 13C, which is distant from the M ions, was found to decrease upon increasing the mass of the M ion, unlike in the case of the Ea values for 1H.
Carbon Paper as Current Collectors in Graphene Hydrogel Electrodes for High-Performance Supercapacitors
Authors: Luo Peihui , Huang Lili
Published: April 2020

Current collectors are an important component of electrodes, functioning as conductive media by collecting currents from active materials and then exporting them to the external circuit. Common current collectors for graphene hydrogel (GH)-based supercapacitors are nickel foams or metal foils (platinum, gold, and aluminium, etc.). Here, hydrothermally synthesized GH was directly pressed on carbon paper and used as electrodes (denoted as GHE) for supercapacitors. With a mass loading of 2.7 mg·cm−2 at an active area of 0.64 cm2, the GHE-based supercapacitors revealed a high gravimetric capacitance of 294 F·g−1 at a current density of 1.18 A·g−1. When increasing the current density to 28.24 A·g−1, 66% (193 F·g−1) of the initial capacitance was maintained for the GHE-based supercapacitors. High performance for GHE-based supercapacitors was attributed to large specific surface area and good electrical conductivity of GH, and its intimate contact with carbon paper.
Adsorption Performance for Reactive Blue 221 Dye of β-Chitosan/Polyamine Functionalized Graphene Oxide Hybrid Adsorbent with High Acid–Alkali Resistance Stability in Different Acid–Alkaline Environments
Authors: Chiu Chih-Wei , Wu Ming-Tsung , Lin Chih-Lung , Li Jia-Wun , Huang Chen-Yang , Soong Yu-Chian , Lee Jimmy Chi-Min , Lee Sanchez William Anderson , Lin Hsuan-Yu
Published: April 2020

A hybrid material obtained by blending β-chitosan (CS) with triethylenetetramine-functionalized graphene oxide (TFGO) (CSGO), was used as an adsorbent for a reactive dye (C.I. Reactive Blue 221 Dye, RB221), and the adsorption and removal performances of unmodified CS and mix-modified CSGO were investigated and compared systematically at different pH values (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, and 12). The adsorption capacities of CS and CSGO were 45.5 and 56.1 mg/g, respectively, at a pH of 2 and 5.4 and 37.2 mg/g, respectively, at a pH of 12. This indicates that TFGO was successfully introduced into CSGO, enabling π–π interactions and electrostatic attraction with the dye molecules. Additionally, benzene ring-shaped GO exhibited a high surface chemical stability, which was conducive to maintaining the stability of the acid and alkali resistance of the CSGO adsorbent. The RB221 adsorption performance of CS and CSGO at acidic condition (pH 3) and alkaline condition (pH 12) and different temperatures was investigated by calculating the adsorption kinetics and isotherms of adsorbents. Overall, the adsorption efficiency of CSGO was superior to that of CS; thus, CSGO is promising for the treatment of dye effluents in a wide pH range.
Transcriptomics in Toxicogenomics, Part I: Experimental Design, Technologies, Publicly Available Data, and Regulatory Aspects
Authors: Kinaret Pia Anneli Sofia , Serra Angela , Federico Antonio , Kohonen Pekka , Nymark Penny , Liampa Irene , Ha My Kieu , Choi Jang-Sik , Jagiello Karolina , Sanabria Natasha , Melagraki Georgia , Cattelani Luca , Fratello Michele , Sarimveis Haralambos , Afantitis Antreas , Yoon Tae-Hyun , Gulumian Mary , Grafström Roland , Puzyn Tomasz , Greco Dario
Published: April 2020

The starting point of successful hazard assessment is the generation of unbiased and trustworthy data. Conventional toxicity testing deals with extensive observations of phenotypic endpoints in vivo and complementing in vitro models. The increasing development of novel materials and chemical compounds dictates the need for a better understanding of the molecular changes occurring in exposed biological systems. Transcriptomics enables the exploration of organisms’ responses to environmental, chemical, and physical agents by observing the molecular alterations in more detail. Toxicogenomics integrates classical toxicology with omics assays, thus allowing the characterization of the mechanism of action (MOA) of chemical compounds, novel small molecules, and engineered nanomaterials (ENMs). Lack of standardization in data generation and analysis currently hampers the full exploitation of toxicogenomics-based evidence in risk assessment. To fill this gap, TGx methods need to take into account appropriate experimental design and possible pitfalls in the transcriptomic analyses as well as data generation and sharing that adhere to the FAIR (Findable, Accessible, Interoperable, and Reusable) principles. In this review, we summarize the recent advancements in the design and analysis of DNA microarray, RNA sequencing (RNA-Seq), and single-cell RNA-Seq (scRNA-Seq) data. We provide guidelines on exposure time, dose and complex endpoint selection, sample quality considerations and sample randomization. Furthermore, we summarize publicly available data resources and highlight applications of TGx data to understand and predict chemical toxicity potential. Additionally, we discuss the efforts to implement TGx into regulatory decision making to promote alternative methods for risk assessment and to support the 3R (reduction, refinement, and replacement) concept. This review is the first part of a three-article series on Transcriptomics in Toxicogenomics. These initial considerations on Experimental Design, Technologies, Publicly Available Data, Regulatory Aspects, are the starting point for further rigorous and reliable data preprocessing and modeling, described in the second and third part of the review series.
Nanoscale Characterization of Surface Plasmon-Coupled Photoluminescence Enhancement in Pseudo Micro Blue LEDs Using Near-Field Scanning Optical Microscopy
Authors: Li Yufeng , Li Aixing , Zhang Ye , Hu Peng , Du Wei , Su Xilin , Li Qiang , Yun Feng
Published: April 2020

The microcave array with extreme large aspect ratio was fabricated on the p-GaN capping layer followed by Ag nanoparticles preparation. The coupling distance between the dual-wavelength InGaN/GaN multiple quantum wells and the localized surface plasmon resonance was carefully characterized in nanometer scale by scanning near-field optical microscopy. The effects of coupling distance and excitation power on the enhancement of photoluminescence were investigated. The penetration depth was measured in the range of 39–55 nm depending on the excitation density. At low excitation power density, the maximum enhancement of 103 was achieved at the optimum coupling distance of 25 nm. Time-resolved photoluminescence shows that the recombination life time was shortened from 5.86 to 1.47 ns by the introduction of Ag nanoparticle plasmon resonance.
Preparation and Properties of Cassava Residue Cellulose Nanofibril/Cassava Starch Composite Films
Authors: Huang Lijie , Zhao Hanyu , Yi Tan , Qi Minghui , Xu Hao , Mo Qi , Huang Chongxing , Wang Shuangfei , Liu Yang
Published: April 2020

Because of its non-toxic, pollution-free, and low-cost advantages, environmentally-friendly packaging is receiving widespread attention. However, using simple technology to prepare environmentally-friendly packaging with excellent comprehensive performance is a difficult problem faced by the world. This paper reports a very simple and environmentally-friendly method. The hydroxyl groups of cellulose nanofibrils (CNFs) were modified by introducing malic acid and the silane coupling agent KH-550, and the modified CNF were added to cassava starch as a reinforcing agent to prepare film with excellent mechanical, hydrophobic, and barrier properties. In addition, due to the addition of malic acid and a silane coupling agent, the dispersibility and thermal stability of the modified CNFs became significantly better. By adjusting the order of adding the modifiers, the hydrophobicity of the CNFs and thermal stability were increased by 53.5% and 36.9% ± 2.7%, respectively. At the same time, the addition of modified CNFs increased the tensile strength, hydrophobicity, and water vapor transmission coefficient of the starch-based composite films by 1034%, 129.4%, and 35.95%, respectively. This material can be widely used in the packaging of food, cosmetics, pharmaceuticals, and medical consumables.
Extra-Virgin Olive Oil from Apulian Cultivars and Intestinal Inflammation
Authors: Cariello Marica , Contursi Annalisa , Gadaleta Raffaella Maria , Piccinin Elena , De Santis Stefania , Piglionica Marilidia , Spaziante Ada Fiorenza , Sabbà Carlo , Villani Gaetano , Moschetta Antonio
Published: April 2020

Inflammatory bowel disease (IBD) is a multifactorial intestinal disorder characterized by chronic intestinal inflammation. The etiology of IBD is still unclear, although genetic, environmental and host factors have been associated to the disease. Extra-virgin olive oil (EVO) is a central component of the Mediterranean diet and it decreases chronic inflammation by interfering with arachidonic acid and NF-κB signaling pathways. Specifically, the different components of EVO are able to confer advantages in terms of health in their site of action. For instance, oleic acid displays a protective effect in liver dysfunction and gut inflammation, whereas phenolic compounds protect colon cells against oxidative damage and improve the symptoms of chronic inflammation in IBD. Given the biological properties of EVO, we investigated whether its administration is able to confer protection in a mouse model of dextrane sodium sulfate (DSS)-induced colitis. Four EVO cultivars from the Apulian Region of Italy, namely Ogliarola (Cima di Bitonto), Coratina, Peranzana and Cima di Mola, respectively, were used. Administration of EVO resulted in reduced body weight loss in our colitis model. Furthermore, mice treated with Ogliarola, Coratina and Cima di Mola EVO displayed a reduction of rectal bleeding and IL-1β, TGFβ, IL-6 gene expression levels. Furthermore, Ogliarola, Coratina and Peranzana EVO administration ameliorated intestinal permeability and histopathological features of inflammation. Our data further validate the well-known positive effects of EVO supplementation in promoting human health and suggest the bona fide contribution of EVO in preventing onset and reducing progression of intestinal inflammation.
Characterization and Mathematical Modeling of Alginate/Chitosan-Based Nanoparticles Releasing the Chemokine CXCL12 to Attract Glioblastoma Cells
Authors: Gascon Suzanne , Giraldo Solano Angéla , El Kheir Wiam , Therriault Hélène , Berthelin Pierre , Cattier Bettina , Marcos Bernard , Virgilio Nick , Paquette Benoit , Faucheux Nathalie , Lauzon Marc-Antoine
Published: April 2020

Chitosan (Chit) currently used to prepare nanoparticles (NPs) for brain application can be complexed with negatively charged polymers such as alginate (Alg) to better entrap positively charged molecules such as CXCL12. A sustained CXCL12 gradient created by a delivery system can be used, as a therapeutic approach, to control the migration of cancerous cells infiltrated in peri-tumoral tissues similar to those of glioblastoma multiforme (GBM). For this purpose, we prepared Alg/Chit NPs entrapping CXCL12 and characterized them. We demonstrated that Alg/Chit NPs, with an average size of ~250 nm, entrapped CXCL12 with ~98% efficiency for initial mass loadings varying from 0.372 to 1.490 µg/mg NPs. The release kinetic profiles of CXCL12 were dependent on the initial mass loading, and the released chemokine from NPs after seven days reached 12.6%, 32.3%, and 59.9% of cumulative release for initial contents of 0.372, 0.744, and 1.490 µg CXCL12/mg NPs, respectively. Mathematical modeling of released kinetics showed a predominant diffusive process with strong interactions between Alg and CXCL12. The CXCL12-NPs were not toxic and did not promote F98 GBM cell proliferation, while the released CXCL12 kept its chemotaxis effect. Thus, we developed an efficient and tunable CXCL12 delivery system as a promising therapeutic strategy that aims to be injected into a hydrogel used to fill the cavity after surgical tumor resection. This system will be used to attract infiltrated GBM cells prior to their elimination by conventional treatment without affecting a large zone of healthy brain tissue.
Impact of Up-Scheduling Medicines on Pharmacy Personnel, Using Codeine as an Example, with Possible Adaption to Complementary Medicines: A Scoping Review
Authors: Lee Kristenbella AYR , Harnett Joanna E. , Ung Carolina Oi Lam , Chaar Betty
Published: April 2020

Within Australia, vitamins, minerals, nutritional supplements, essential oils, and homoeopathic and herbal preparations are collectively termed and regulated as Complementary Medicines (CMs) by the Australian Therapeutic Goods Administration (TGA). CMs are predominantly self-selected through a pharmacy, providing pharmacy personnel an opportunity to engage with the public about their CM use. CMs are currently non-scheduled products in Australia. This review aimed to summarize the literature reporting the potential effect on pharmacies if scheduling of CMs was adopted, using codeine as an example. A scoping review methodology was employed. Seven databases were searched to identify four key concepts, including: CMs, scheduling and rescheduling, codeine, and pharmacists. Seven studies were included for analysis. The majority of the literature has explored qualitative studies on the perception and opinion of pharmacists in relation to the up-scheduling of codeine. The case of codeine illustrates the possible impact of up-scheduling. If CMs were to be up-scheduled, the accessibility of CMs would be limited to the pharmacy providing a role for pharmacy personnel, including both pharmacists and pharmacy technicians, to counsel on CM use. However, careful collaboration and consideration on how such a regulatory change would impact other key-stakeholders, including CM practitioners, requires both a strategic and collaborative approach.
Effects of Starter Defect on Energy Release Rate of Three-Point End-Notch Flexure Tested Unidirectional Carbon Fiber Reinforced Polymer Composite
Authors: Nor Fethma M. , Lim Joong Yeon , Tamin Mohd Nasir , Lee Ho Yong , Kurniawan D.
Published: April 2020

The mechanics of damage and fracture process in unidirectional carbon fiber reinforced polymer (CFRP) composites subjected to shear loading (Mode II) were examined using the experimental method of the three-point end-notch flexure (3ENF) test. The CFRP composite consists of [0o]16 with an insert film in the middle plane for a starter defect. A 3ENF test sample with a span of 50 mm and interface delamination crack length of 12.5 mm was tested to yield the load vs. deformation response. A sudden load drop observed at maximum force value indicates the onset of delamination crack propagation. The results are used to extract the energy release rate, GIIC, of the laminates with an insert film starter defect. The effect of the starter defect on the magnitude of GIIC was examined using the CFRP composite sample with a Mode II delamination pre-crack. The higher magnitude of GIIC for the sample with insert film starter defect was attributed to the initial straight geometry of the notch/interface crack and the toughness of the resin at the notch front of the fabricated film insert. The fractured sample was examined using a micro-computerized tomography scanner to establish the shape of the internal delamination crack front. Results revealed that the interface delamination propagated in a non-uniform manner, leaving a curved-shaped crack profile.
Design and Synthesis of Polysiloxane Based Side Chain Liquid Crystal Polymer for Improving the Processability and Toughness of Magnesium Hydrate/Linear Low-Density Polyethylene Composites
Authors: Guan Xiaoxiao , Cao Bo , Cai Jianan , Ye Zhenxing , Lu Xiang , Huang Haohao , Liu Shumei , Zhao Jianqing
Published: April 2020

In this study, a polysiloxane grafted by thermotropic liquid crystal polymer (PSCTLCP) is designed and synthesized to effectively improve the processability and toughness of magnesium hydroxide (MH)/linear low-density polyethylene (LLDPE) composites. The obtained PSCTLCP is a nematic liquid crystal polymer; the liquid crystal phase exists in a temperature range of 170 to 275 °C, and its initial thermal decomposition temperature is as high as 279.6 °C, which matches the processing temperature of MH/LLDPE composites. With the increase of PSCTLCP loading, the balance melt torque of MH/LLDPE/PSCTLCP composites is gradually decreased by 42% at 5 wt % PSCTLCP loading. Moreover, the power law index of MH/LLDPE/PSCTLCP composite melt is smaller than 1, but gradually increased with PSCTLCP, the flowing activation energy of PSCTLCP-1.0 is lower than that of MH/LLDPE at the same shear rate, indicating that the sensitivity of apparent melt viscosity of the composites to shear rate and to temperature is decreased with the increase of PSCTLCP, and the processing window is broadened by the addition of PSCTLCP. Besides, the elongation at break of MH/LLDPE/PSCTLCP composites increases from 6.85% of the baseline MH/LLDPE to 17.66% at 3 wt % PSCTLCP loading. All the results indicate that PSCTLCP can significantly improve the processability and toughness of MH/LLDPE composites.
Preparation of Biomass-Based Ester End-Capped Hyperbranched Poly(ether)s via Facile One-Pot Reaction and Their Performance as Non-Toxic Plasticizers
Authors: Li Qiaoguang , Shu Xugang , Jia Puyou , Zhou Yonghong
Published: April 2020

The aim of this study was to develop a facile one-pot reaction for the synthesis of biomass-based hyperbranched poly(ether)s end-capped as acetate esters (BHE) for use as a sustainable, safe and feasible plasticizer for flexible poly(vinyl chloride) (PVC) materials. BHE is completely miscible with PVC but shows weaker plasticizing effect than dioctyl phthalate (DOP) (EΔTg value of BHE reaches 64.8%). PVC plasticized with BHE displays greater thermal stability than that of PVC or PVC plasticized with DOP materials. BHE improves the thermal stability and flexibility of PVC materials. As a plasticizer, BHE displays lower solvent extractability and greater volatilization resistance than DOP. Acute oral toxicity indicates that BHE has toxic doses of 5 g/kg, suggesting that BHE is non-toxic.
Electrolytic Oxidation as a Sustainable Method to Transform Urine into Nutrients
Authors: Bensalah Nasr , Dbira Sondos , Bedoui Ahmed , Ahmad Mohammad I.
Published: April 2020

In this work, the transformation of urine into nutrients using electrolytic oxidation in a single-compartment electrochemical cell in galvanostatic mode was investigated. The electrolytic oxidation was performed using thin film anode materials: boron-doped diamond (BDD) and dimensionally stable anodes (DSA). The transformation of urine into nutrients was confirmed by the release of nitrate (NO3−) and ammonium (NH4+) ions during electrolytic treatment of synthetic urine aqueous solutions. The removal of chemical oxygen demand (COD) and total organic carbon (TOC) during electrolytic treatment confirmed the conversion of organic pollutants into biocompatible substances. Higher amounts of NO3− and NH4+ were released by electrolytic oxidation using BDD compared to DSA anodes. The removal of COD and TOC was faster using BDD anodes at different current densities. Active chlorine and chloramines were formed during electrolytic treatment, which is advantageous to deactivate any pathogenic microorganisms. Larger quantities of active chlorine and chloramines were measured with DSA anodes. The control of chlorine by-products to concentrations lower than the regulations require can be possible by lowering the current density to values smaller than 20 mA/cm2. Electrolytic oxidation using BDD or DSA thin film anodes seems to be a sustainable method capable of transforming urine into nutrients, removing organic pollution, and deactivating pathogens.
Editorial for the Special Issue “Remote Sensing of Large Rivers”
Authors: Alcântara Enner , Park Edward
Published: April 2020

Large rivers play important roles on Earth, such as transporting eroded materials from the continents to the ocean, facilitating the transfer of nutrients through biogeochemical cycles, and sustaining complex ecosystems and high levels of biodiversity [...]
Monitoring Plant Functional Diversity Using the Reflectance and Echo from Space
Authors: Ma Xuanlong , Migliavacca Mirco , Wirth Christian , Bohn Friedrich J. , Huth Andreas , Richter Ronny , Mahecha Miguel D.
Published: April 2020

Plant functional diversity (FD) is an important component of biodiversity. Evidence shows that FD strongly determines ecosystem functioning and stability and also regulates various ecosystem services that underpin human well-being. Given the importance of FD, it is critical to monitor its variations in an explicit manner across space and time, a highly demanding task that cannot be resolved solely by field data. Today, high hopes are placed on satellite-based observations to complement field plot data. The promise is that multiscale monitoring of plant FD, ecosystem functioning, and their services is now possible at global scales in near real-time. However, non-trivial scale challenges remain to be overcome before plant ecology can capitalize on the latest advances in Earth Observation (EO). Here, we articulate the existing scale challenges in linking field and satellite data and further elaborated in detail how to address these challenges via the latest innovations in optical and radar sensor technologies and image analysis algorithms. Addressing these challenges not only requires novel remote sensing theories and algorithms but also urges more effective communication between remote sensing scientists and field ecologists to foster mutual understanding of the existing challenges. Only through a collaborative approach can we achieve the global plant functional diversity monitoring goal.
An Integrated Shadow-Adjusted Snow-Aging Index for Alpine Regions
Authors: Li Haixing , Liu Jinrong , Bu Xiangxu , Feng Xuezhi , Xiao Pengfeng
Published: April 2020

Detecting the variations in snow cover aging over undulating alpine regions is challenging owing to the complex snow-aging process and shadow effect from steep slopes. This study proposes a novel snow-cover status index, namely shadow-adjusted snow-aging index (SASAI), portraying the integrated aging process within the Manas River Basin in northwest China. The Environment Satellites HJ-1A/B optical images and in-field measurements were used during the snow ablation and accumulation periods. The in-field measurements provide a reference for building a candidate library of snow-aging indicators. The representative aging samples for training and validation were obtained using the proposed time-gap searching method combined with the target zones established based on the altitude of snowline. An analytic hierarchy process was used to determine the snow-aging index (SAI) using multiple optimal snow-aging indicators. After correction by the extreme value optimization algorithm, the SASAI was finally corrected for the effects of shading and assessed. This study provides both a flexible algorithm that indicates the characteristics of snow aging and speculation on the causes of the aging process. The separability of the SAI/SASAI and adaptability of this algorithm on multiperiod remote sensing images further demonstrates the applicability of the SASAI to all the alpine regions.
Predicting Loneliness from Where and What People Do
Authors: MacDonald Kristi J. , Willemsen Gonneke , Boomsma Dorret I. , Schermer Julie Aitken
Published: April 2020

The many devastating mental health outcomes associated with chronic loneliness is the motivation behind research into examining personal and demographic characteristics of the lonely. The present study sought to examine the connection of where people live (degree of urbanization) and what people do (leisure activities) with self-report of loneliness in a large sample (N = 8356) of unrelated Dutch adults. Information regarding where people live and what they do in their leisure time was entered into a regression analysis for self-reported loneliness. The overall regression was significant and accounted for 2.8% of the loneliness scale scores. Significant independent predictors for loneliness were living in heavily urbanized areas and engaging in fewer social activities. People who went sightseeing or to amusement parks/zoos or who participated in clubs reported being less lonely. Spending time using a computer predicted higher self-report loneliness scores. Consistent with previous research, after controlling for other variables, gender was not a significant predictor of loneliness but both a younger age and a curvilinear or U-shaped curve of age predicted loneliness (the younger and the much older). The results suggest that meaningful interpersonal interactions may result in lower feelings of loneliness.