bluuebunny/crossref_metadata_2025 huggingface dataset:
An Improved Current Signal Extraction-Based High-Frequency Pulsating Square-Wave Voltage Injection Method for Interior Permanent-Magnet Synchronous Motor Position-Sensorless Control
Authors: Meng Dongyi , Wu Qiya , Zhang Jia , Diao Lijun
Published: June 2024

The high-frequency (HF) voltage injection method is widely applied in achieving position-sensorless control for interior permanent-magnet synchronous motors (IPMSMs). This method necessitates precise and rapid extraction of the current signal for accurate position estimation and field-oriented control (FOC). In the traditional methods, the position error signal and fundamental current are extracted from the current signal using band-pass filters (BPFs) and low-pass filters (LPFs), or a method based on time-delay filters. However, the traditional extraction method falls short in ensuring simultaneous dynamic performance and accuracy, particularly when the switching frequency is limited or when encountering harmonic and noise interference. In this article, a novel HF pulsating square-wave voltage injection method based on an improved current signal-extraction strategy is proposed to improve the extraction accuracy while maintaining good dynamic performance. The newly devised current signal-extraction method is crafted upon a notch filter (NF). Through harnessing NF’s effective separation characteristics of specific frequency signals, the current signal is meticulously processed. This process yields the extraction of the position error signal and fundamental-current component, crucial for accurate position estimation and motor FOC. Simulation and hardware-in-the-loop (HIL) testing are conducted to validate the effectiveness of the proposed approach.
AI-Enhanced Tools and Strategies for Airborne Disease Prevention in Cultural Heritage Sites
Authors: Greco Enrico , Gaetano Anastasia Serena , De Spirt Alessia , Semeraro Sabrina , Piscitelli Prisco , Miani Alessandro , Mecca Saverio , Karaj Stela , Trombin Rita , Hodgton Rachel , Barbieri Pierluigi
Published: June 2024

In the wake of the COVID-19 pandemic, the surveillance and safety measures of indoor Cultural Heritage sites have become a paramount concern due to the unique challenges posed by their enclosed environments and high visitor volumes. This communication explores the integration of Artificial Intelligence (AI) in enhancing epidemiological surveillance and health safety protocols in these culturally significant spaces. AI technologies, including machine learning algorithms and Internet of Things (IoT) sensors, have shown promising potential in monitoring air quality, detecting pathogens, and managing crowd dynamics to mitigate the spread of infectious diseases. We review various applications of AI that have been employed to address both direct health risks and indirect impacts such as visitor experience and preservation practices. Additionally, this paper discusses the challenges and limitations of AI deployment, such as ethical considerations, privacy issues, and financial constraints. By harnessing AI, Cultural Heritage sites can not only improve their resilience against future pandemics but also ensure the safety and well-being of visitors and staff, thus preserving these treasured sites for future generations. This exploration into AI’s role in post-COVID surveillance at Cultural Heritage sites opens new frontiers in combining technology with traditional conservation and public health efforts, providing a blueprint for enhanced safety and operational efficiency in response to global health challenges.
Investigation of Malocclusion and Associated Factors in Preschoolers: A Cross-Sectional Questionnaire Study
Authors: Carvalho Amanda Araújo de , Almeida Tatiana Frederico de , Cabral Maria Beatriz Barreto de Sousa , Cangussu Maria Cristina Teixeira
Published: June 2024

This study aims to describe the prevalence of malocclusion and identify associated factors in preschool children. Completed in 2022–2023, this cross-sectional study included 523 children aged 26 to 80 months in municipal schools in Salvador. An oral examination was carried out on the children, and a questionnaire was self-administered by the parents. Descriptive analyses and multivariate logistic regression (the backward method, p-value ≤ 0.05, 95% CI) were conducted. The majority of children were female (51.82%), over 54 months old (52.2%), Black or mixed race (90.63%), and not affected by COVID-19 (92.35%). The prevalence of malocclusion was 43.21%, with open bite as the most common condition. There was a significant association between malocclusion and screen time (OR: 1.34; p: 0.116; CI: 1.0–1.94), physical/psychological aggression (OR: 2.55; p: 0.031; CI: 1.0–5.98), consumption of ultra-processed foods (OR: 1.77; p: 0.003; CI: 1.22–2.57), digital suction (OR: 3.1; p: 0.001; CI: 1.56–6.16), and the habit of biting objects (OR: 1.56; p: 0.121; CI: 1.0–2.73). The promotion of comprehensive health in early childhood and psychosocial interventions are recommended, aiming to reduce screen time, aggression, consumption of ultra-processed foods, thumb sucking, and the habit of biting objects to prevent malocclusion.
Seasonal Dynamics of Greenhouse Gas Emissions from Island-like Forest Soils in the Sanjiang Plain: Impacts of Soil Characteristics and Climatic Factors
Authors: Xu Nan , Li Jinbo , Zhong Haixiu , Wang Yuan , Dong Juexian , Yang Xuechen
Published: June 2024

Using the static chamber–gas chromatography method, this study investigates the flux characteristics of CO2, CH4, and N2O in the soils of three typical island-like forests in the Sanjiang Plain during the growing season (May to September), as well as their relationships with environmental factors. The results indicate that the soils of the Broadleaf mixed forest, Quercus mongolica forest, and Betula platyphylla forest act as emission sources for CO2 and N2O, with average fluxes of 433.92, 452.41, and 358.17 μg·m−2·h−1 for CO2 and 12.48, 13.02, and 10.51 μg·m−2·h−1 for N2O, respectively. The differences among forest types are not significant. All three forest types serve as sinks for CH4, with average fluxes of −22.52, −23.29, and −0.76 μg·m−2·h−1. The Betula platyphylla forest has a significantly weaker absorption intensity compared to the other types (p < 0.01). The measured environmental factors collectively explain 66.58% of the variability in greenhouse gas fluxes in the island-like forests, with soil temperature, soil moisture, and total nitrogen content being the main influencing factors in the region. Rising temperatures favor the emission of CO2 and N2O and the absorption of CH4 in all three forest types. Increased soil moisture inhibits the absorption of CH4 in the Broadleaf mixed forest and Quercus mongolica forest, while higher levels of alkali-hydrolyzed nitrogen enhance the N2O flux in the Quercus mongolica forest. Soil organic carbon and soil pH significantly influence only the greenhouse gas fluxes of the Betula platyphylla forest.
Advanced RBF Methods for Mapping Aerodynamic Loads onto Structures in High-Fidelity FSI Simulations
Authors: Chiappa Andrea , Lopez Andrea , Groth Corrado
Published: June 2024

The reliable exchange of data is a crucial issue for the loose coupling of computational fluid dynamics (CFD) and computational structural mechanics (CSM) modules in fluid–structure interaction (FSI) applications. This paper presents a comparison between two methods for mapping the traction field across mismatching grids, namely the RIBES method and the preCICE algorithm, both based on radial basis function (RBF) interpolation. The two methods demonstrate different degrees of control over balance preservation during mapping, with the RIBES algorithm exhibiting greater efficacy. Test benches are a parametric double curved geometry and a wind tunnel mock-up. In this second case, forces from mapping are used to load a CSM model to retrieve stress and displacement fields. Differences in FEM results are appreciable although not significant, showing a correlation between the accuracy of balance preservation during data mapping and the structural output.
Study on Adsorption of Cd in Solution and Soil by Modified Biochar–Calcium Alginate Hydrogel
Authors: Wang Shuyue , Wang Yajun , Wang Xinyi , Sun Sijia , Zhang Yanru , Jiao Weixiong , Lin Dasong
Published: June 2024

Contamination with cadmium (Cd) is a prominent issue in agricultural non-point source pollution in China. With the deposition and activation of numerous Cd metal elements in farmland, the problem of excessive pollution of agricultural produce can no longer be disregarded. Considering the issue of Cd pollution in farmland, this study proposes the utilization of cross-linked modified biochar (prepared from pine wood) and calcium alginate hydrogels to fabricate a composite material which is called MB-CA for short. The aim is to investigate the adsorption and passivation mechanism of soil Cd by this innovative composite. The MB-CA exhibits a higher heavy metal adsorption capacity compared to traditional biochar and hydrogel due to its increased oxygen-containing functional groups and heavy metal adsorption sites. In the Cd solution adsorption experiment, the highest Cd2+ removal rate reached 85.48%. In addition, it was found that the material also has an excellent pH improvement effect. Through the adsorption kinetics experiment and the soil culture experiments, it was determined that MB-CA adheres to the quasi-second-order kinetic model and is capable of adsorbing 35.94% of Cd2+ in soil. This study validates the efficacy of MB-CA in the adsorption and passivation of Cd in soil, offering a novel approach for managing Cd-contaminated cultivated land.
The Analysis, Description, and Examination of the Maize LAC Gene Family’s Reaction to Abiotic and Biotic Stress
Authors: Wang Tonghan , Liu Yang , Zou Kunliang , Guan Minhui , Wu Yutong , Hu Ying , Yu Haibing , Du Junli , Wu Degong
Published: June 2024

Laccase (LAC) is a diverse group of genes found throughout the plant genome essential for plant growth and the response to stress by converting monolignin into intricate lignin formations. However, a comprehensive investigation of maize laccase has not yet been documented. A bioinformatics approach was utilized in this research to conduct a thorough examination of maize (Zea mays L.), resulting in the identification and categorization of 22 laccase genes (ZmLAC) into six subfamilies. The gene structure and motifs of each subgroup were largely consistent. The distribution of the 22 LAC genes was uneven among the maize chromosomes, with the exception of chromosome 9. The differentiation of the genes was based on fragment replication, and the differentiation time was about 33.37 million years ago. ZmLAC proteins are primarily acidic proteins. There are 18 cis-acting elements in the promoter sequences of the maize LAC gene family associated with growth and development, stress, hormones, light response, and stress response. The analysis of tissue-specific expression revealed a high expression of the maize LAC gene family prior to the V9 stage, with minimal expression at post-V9. Upon reviewing the RNA-seq information from the publicly available transcriptome, it was discovered that ZmLAC5, ZmLAC10, and ZmLAC17 exhibited significant expression levels when exposed to various biotic and abiotic stress factors, suggesting their crucial involvement in stress responses and potential value for further research. This study offers an understanding of the functions of the LAC genes in maize’s response to biotic and abiotic stress, along with a theoretical basis for comprehending the molecular processes at play.
Selection of the Value of the Power Distance Exponent for Mapping with the Inverse Distance Weighting Method—Application in Subsurface Porosity Mapping, Northern Croatia Neogene
Authors: Barudžija Uroš , Ivšinović Josip , Malvić Tomislav
Published: June 2024

The correct selection of the value of p is a complex and iterative procedure that requires experience in the interpretation of the obtained interpolated maps. Inverse Distance Weighting is a method applied to the porosities of the K and L hydrocarbon reservoirs discovered in the Neogene (Lower Pontian) subsurface sandstones in northern Croatia (Pannonian Basin System). They represent small and large data samples. Also, a standard statistical analysis of the data was made, followed by a qualitative–quantitative analysis of the maps, based on the selection of different values for the power distance exponent (p-value) for the K and L reservoir maps. According to the qualitative analysis, for a small data set, the p-value could be set at 1 or 2, giving the optimal result, while for a large data set, a p value of 3 or 4 could be applied. For quantitative analysis, in the case of a small data set, p = 2 is recommended, resulting in a root mean square error value of 0.03458, a mean absolute error of 0.02013 and a median absolute deviation of 0.00546. In contrast, a p-value of 3 or 4 is selected as appropriate for a large data set, with root mean square errors of 0.02435 and 0.02437, mean square errors of 0.01582 and 0.01509 and median absolute deviations 0.00896 and 0.00444. Eventually for a small data set, it is recommended to use a p-value of 2, and for a large data set, a p-value of 3 or 4.
Exploring the Genetic Landscape of Tilia Spp. with Molecular and Statistical Tools
Authors: Tenche-Constantinescu Alina-Maria , Lalescu Dacian Virgil , Popescu Sorina , Sarac Ioan , Petrescu Irina , Petolescu Cerasela , Camen Dorin , Horablaga Adina , Popescu Cosmin Alin , Berar Cristian , Onisan Emilian
Published: June 2024

Tree species with high ecological plasticity are recommended for use in urban green infrastructures. This study explores the genetic diversity of Tilia cordata, Tilia platyphyllos, and Tilia argentea, highlighting their contribution to urban landscapes. In this respect, the genetic variability of individuals from four populations of the Tilia genus has been analyzed using Inter Simple Sequence Repeats (ISSR) molecular markers, and some of their phenotypic characters were naked-eyed observed or determined by dendrometric measurements. Significant variability between the populations studied was determined. Cluster analysis identified two main groups: Tilia cordata I and Tilia cordata II populations formed the first cluster, and Tilia platyphyllos and Tilia argentea populations formed the second cluster. The results of the phenotypic analysis confirmed the genetic results separating the two populations of Tilia cordata from the populations of Tilia platyphyllos and Tilia argentea in relation to their visible morphological characters. Results obtained from dendrometric measurements analysis represent the foundation for further investigations on urban ecology. An essential potential practical use of the results: the establishment of more efficient ISSR primers to identify the Tilia species analyzed: UBC864, A12, UBC840, and A7 for Tilia cordata, A13 for Tilia platyphyllos and A7 for Tilia argentea has been demonstrated.
Ethanol Extract of the Microalga Phaeodactylum tricornutum Shows Hepatoprotective Effects against Acetaminophen-Induced Acute Liver Injury in Mice
Authors: Kim Dae Yoon , Park Hui Jin , Eom Jae-In , Han Cheol-Ho , Pan Cheol-Ho , Lee Jae Kwon
Published: June 2024

Acute liver failure is an infrequent yet fatal condition marked by rapid liver function decline, leading to abnormalities in blood clotting and cognitive impairment among individuals without prior liver ailments. The primary reasons for liver failure are infection with hepatitis virus or overdose of certain medicines, such as acetaminophen. Phaeodactylum tricornutum (PT), a type of microalgae known as a diatom species, has been reported to contain an active ingredient with anti-inflammatory and anti-obesity effects. In this study, we evaluated the preventive and therapeutic activities of PT extract in acute liver failure. To achieve our purpose, we used two different acute liver failure models: acetaminophen- and D-GalN/LPS-induced acute liver failure. PT extract showed protective activity against acetaminophen-induced acute liver failure through attenuation of the inflammatory response. However, we failed to demonstrate the protective effects of PT against acute liver injury in the D-GalN/LPS model. Although the PT extract did not show protective activity against two different acute liver failure animal models, this study clearly demonstrates the importance of considering the differences among animal models when selecting an acute liver failure model for evaluation.
Associations between Serum Kallistatin Levels and Markers of Glucose Homeostasis, Inflammation, and Lipoprotein Metabolism in Patients with Type 2 Diabetes and Nondiabetic Obesity
Authors: Lőrincz Hajnalka , Csiha Sára , Ratku Balázs , Somodi Sándor , Sztanek Ferenc , Paragh György , Harangi Mariann
Published: June 2024

Kallistatin is an endogenous serine proteinase inhibitor with various functions, including antioxidative, anti-inflammatory, and anti-atherosclerotic properties. To date, associations between kallistatin and lipoprotein subfractions are poorly investigated. In this study, we enrolled 62 obese patients with type 2 diabetes (T2D), 106 nondiabetic obese (NDO) subjects matched in gender, age, and body mass index, as well as 49 gender- and age-matched healthy, normal-weight controls. Serum kallistatin levels were measured with ELISA, and lipoprotein subfractions were analyzed using Lipoprint® (Quantimetrix Corp., Redondo Beach, CA, USA) gel electrophoresis. Kallistatin concentrations were significantly higher in T2D patients compared to NDO and control groups. We found significant positive correlations between very-low-density lipoprotein (VLDL), small high-density lipoprotein (HDL) subfractions, glucose, hemoglobin A1c (HbA1c), betatrophin, and kallistatin, while negative correlations were detected between mean low-density lipoprotein (LDL) size, large and intermediate HDL subfractions, and kallistatin in the whole study population. The best predictor of kallistatin was HbA1c in T2D patients, high-sensitivity C-reactive protein (hsCRP) and betatrophin in NDO patients, and hsCRP in controls. Our results indicate that kallistatin expression might be induced by persistent hyperglycemia in T2D, while in nondiabetic subjects, its production might be associated with systemic inflammation. The correlation of kallistatin with lipid subfractions may suggest its putative role in atherogenesis.
A Comprehensive Analysis of Non-Desmosomal Rare Genetic Variants in Arrhythmogenic Cardiomyopathy: Integrating in Padua Cohort Literature-Derived Data
Authors: Bueno Marinas Maria , Cason Marco , Bariani Riccardo , Celeghin Rudy , De Gaspari Monica , Pinci Serena , Cipriani Alberto , Rigato Ilaria , Zorzi Alessandro , Rizzo Stefania , Thiene Gaetano , Perazzolo Marra Martina , Corrado Domenico , Basso Cristina , Bauce Barbara , Pilichou Kalliopi
Published: June 2024

Arrhythmogenic cardiomyopathy (ACM) is an inherited myocardial disease at risk of sudden death. Genetic testing impacts greatly in ACM diagnosis, but gene-disease associations have yet to be determined for the increasing number of genes included in clinical panels. Genetic variants evaluation was undertaken for the most relevant non-desmosomal disease genes. We retrospectively studied 320 unrelated Italian ACM patients, including 243 cases with predominant right-ventricular (ARVC) and 77 cases with predominant left-ventricular (ALVC) involvement, who did not carry pathogenic/likely pathogenic (P/LP) variants in desmosome-coding genes. The aim was to assess rare genetic variants in transmembrane protein 43 (TMEM43), desmin (DES), phospholamban (PLN), filamin c (FLNC), cadherin 2 (CDH2), and tight junction protein 1 (TJP1), based on current adjudication guidelines and reappraisal on reported literature data. Thirty-five rare genetic variants, including 23 (64%) P/LP, were identified in 39 patients (16/243 ARVC; 23/77 ALVC): 22 FLNC, 9 DES, 2 TMEM43, and 2 CDH2. No P/LP variants were found in PLN and TJP1 genes. Gene-based burden analysis, including P/LP variants reported in literature, showed significant enrichment for TMEM43 (3.79-fold), DES (10.31-fold), PLN (117.8-fold) and FLNC (107-fold). A non-desmosomal rare genetic variant is found in a minority of ARVC patients but in about one third of ALVC patients; as such, clinical decision-making should be driven by genes with robust evidence. More than two thirds of non-desmosomal P/LP variants occur in FLNC.
Investigating Vitamin D-Binding Protein’s Role in Childhood Health and Development
Authors: Delrue Charlotte , Speeckaert Reinhart , Delanghe Joris R. , Prytuła Agnieszka , Speeckaert Marijn M.
Published: June 2024

Vitamin D-binding protein (DBP), also known as Gc-globulin, is a protein that affects several physiological processes, including the transport and regulation of vitamin D metabolites. Genetic polymorphisms in the DBP gene have a significant impact on vitamin D levels and may have implications for disease risk. DBP polymorphisms are linked to differential immune responses, which could influence the onset of juvenile diseases. This narrative review examines the various roles of DBP, with a focus on bone health, immunological regulation, and lipid metabolism in children. Chronic disorders affected by DBP polymorphisms include bone abnormalities, autoimmune diseases, cardiovascular issues, childhood asthma, allergies, cystic fibrosis, acute liver failure, celiac disease, inflammatory bowel disease, and chronic kidney disease. Future research should focus on identifying the processes that underpin the many roles that DBP plays and developing customized therapeutics to improve health outcomes in the juvenile population.
BRCA1 Intragenic Duplication Combined with a Likely Pathogenic TP53 Variant in a Patient with Triple-Negative Breast Cancer: Clinical Risk and Management
Authors: Ea Vuthy , Berthozat Claudine , Dreyfus Hélène , Legrand Clémentine , Rousselet Estelle , Peysselon Magalie , Baudet Laura , Martinez Guillaume , Coutton Charles , Bidart Marie
Published: June 2024

For patients with hereditary breast and ovarian cancer, the probability of carrying two pathogenic variants (PVs) in dominant cancer-predisposing genes is rare. Using targeted next-generation sequencing (NGS), we investigated a 49-year-old Caucasian woman who developed a highly aggressive breast tumor. Our analyses identified an intragenic germline heterozygous duplication in BRCA1 with an additional likely PV in the TP53 gene. The BRCA1 variant was confirmed by multiplex ligation probe amplification (MLPA), and genomic breakpoints were characterized at the nucleotide level (c.135-2578_442-1104dup). mRNA extracted from lymphocytes was amplified by RT-PCR and then Sanger sequenced, revealing a tandem duplication r.135_441dup; p.(Gln148Ilefs*20). This duplication results in the synthesis of a truncated and, most likely, nonfunctional protein. Following functional studies, the TP53 exon 5 c.472C > T; p.(Arg158Cys) missense variant was classified as likely pathogenic by the Li-Fraumeni Syndrome (LFS) working group. This type of unexpected association will be increasingly identified in the future, with the switch from targeted BRCA sequencing to hereditary breast and ovarian cancer (HBOC) panel sequencing, raising the question of how these patients should be managed. It is therefore important to record and investigate these rare double-heterozygous genotypes.
Ex Vivo Histological Analysis of Corneas with Manually Implanted Intracorneal Stromal Ring Segments
Authors: Kapelushnik Noa , Werner Liliana , Levinger Nadav , Levinger Samuel , Barequet Irina S.
Published: June 2024

Backgrond: Intracorneal ring segments (ICRSs) are utilized to correct refractive changes impacting visual acuity, commonly implanted via femtosecond laser but can also inserted manually. Corneal deposits alongside the ICRS channels are seen commonly. Methods: This study explores the histological characteristics of corneal deposits following manual ICRS implantation, comparing them to previously published articles describing femtosecond laser-assisted cases. Results: This is a retrospective analysis of three cases involving manual ICRS implantation, accumulation of whitish deposits and later explanation of the corneas due to penetrating keratoplasty (PKP). Patient demographics, ocular history, and surgical details were collected. Histological analysis employed Hematoxylin and Eosin (H&E) and Masson’s trichrome staining. Whitish deposits along ICRS tracts were observed in all cases, with minimal fibroblastic transformation of keratocytes adjacent to the segments. Comparing these cases of manual to femtosecond laser-assisted ICRS implantation, in most cases, similar deposits were identified, indicating the deposits’ association with the stromal tissue reaction to the ring segment and not to the surgical technique. Conclusions: This study contributes insights into the histopathology of manually implanted ICRS, emphasizing the shared nature of deposits in both insertion methods. The findings highlight the link between deposits and the stromal tissue reaction to the ring segment, irrespective of the insertion technique.
Use of Real-World FHIR Data Combined with Context-Sensitive Decision Modeling to Guide Sentinel Biopsy in Melanoma
Authors: Beckmann Catharina Lena , Lodde Georg , Swoboda Jessica , Livingstone Elisabeth , Böckmann Britta
Published: June 2024

Background: To support clinical decision-making at the point of care, the “best next step” based on Standard Operating Procedures (SOPs) and actual accurate patient data must be provided. To do this, textual SOPs have to be transformed into operable clinical algorithms and linked to the data of the patient being treated. For this linkage, we need to know exactly which data are needed by clinicians at a certain decision point and whether these data are available. These data might be identical to the data used within the SOP or might integrate a broader view. To address these concerns, we examined if the data used by the SOP is also complete from the point of view of physicians for contextual decision-making. Methods: We selected a cohort of 67 patients with stage III melanoma who had undergone adjuvant treatment and mainly had an indication for a sentinel biopsy. First, we performed a step-by-step simulation of the patient treatment along our clinical algorithm, which is based on a hospital-specific SOP, to validate the algorithm with the given Fast Healthcare Interoperability Resources (FHIR)-based data of our cohort. Second, we presented three different decision situations within our algorithm to 10 dermatooncologists, focusing on the concrete patient data used at this decision point. The results were conducted, analyzed, and compared with those of the pure algorithmic simulation. Results: The treatment paths of patients with melanoma could be retrospectively simulated along the clinical algorithm using data from the patients’ electronic health records. The subsequent evaluation by dermatooncologists showed that the data used at the three decision points had a completeness between 84.6% and 100.0% compared with the data used by the SOP. At one decision point, data on “patient age (at primary diagnosis)” and “date of first diagnosis” were missing. Conclusions: The data needed for our decision points are available in the FHIR-based dataset. Furthermore, the data used at decision points by the SOP and hence the clinical algorithm are nearly complete compared with the data required by physicians in clinical practice. This is an important precondition for further research focusing on presenting decision points within a treatment process integrated with the patient data needed.
Every Patent Foramen Ovale Should Be Closed
Authors: Meier Bernhard
Published: June 2024

At present, the patent foramen ovale (PFO) does not receive the deserved medical attention. The PFO poses a serious threat to health and even the life of mankind. The first respective case report in the medical literature dates back to the 19th century. It led to death. The fact that a PFO is present in roughly 25% of people underscores its overall potential to cause harm. Yet at the same time, the sheer number discourages the medical community from screening for it and from treating it. About 5% of the population have particularly dangerous forms of PFOs. Such PFOs portray a high enough risk for clinical events, the likes of death, stroke, myocardial infarction, or ocular, visceral, and peripheral embolism, to justify screening for them. Highly significant health incidents being at stake, it appears obvious that PFO closure should be used for primary prevention. This is supported by the fact that closing a PFO is the simplest intervention in cardiology, with presumably the highest clinical yield. Being mainly a preventive measure, PFO closure represents a mechanical vaccination. When closing PFOs for one of the rarer therapeutic indications (migraine, platypnea orthodeoxia, etc.), patients automatically profit from the collateral benefit of getting, at the same time, mechanically vaccinated for life against paradoxical embolism. Vice versa, closing a PFO for the prevention of paradoxical embolism betters or cures migraine or exercise dyspnea not infrequently, thereby improving quality of life as a collateral benefit.
Progress in Core–Shell Magnetic Mesoporous Materials for Enriching Post-Translationally Modified Peptides
Authors: Zhu Zhenyu , Fu Hang , Zhao Yu , Yan Qiulin
Published: June 2024

Endogenous peptides, particularly those with post-translational modifications, are increasingly being studied as biomarkers for diagnosing various diseases. However, they are weakly ionizable, have a low abundance in biological samples, and may be interfered with by high levels of proteins, peptides, and other macromolecular impurities, resulting in a high limit of detection and insufficient amounts of post-translationally modified peptides in real biological samples to be examined. Therefore, separation and enrichment are necessary before analyzing these biomarkers using mass spectrometry. Mesoporous materials have regular adjustable pores that can eliminate large proteins and impurities, and their large specific surface area can bind more target peptides, but this may result in the partial loss or destruction of target peptides during centrifugal separation. On the other hand, magnetic mesoporous materials can be used to separate the target using an external magnetic field, which improves the separation efficiency and yield. Core–shell magnetic mesoporous materials are widely utilized for peptide separation and enrichment due to their biocompatibility, efficient enrichment capability, and excellent recoverability. This paper provides a review of the latest progress in core–shell magnetic mesoporous materials for enriching glycopeptides and phosphopeptides and compares their enrichment performance with different types of functionalization methods.
Incidence of Late-Onset Psoriasis Following Tonsillectomy: A Longitudinal Follow-Up Study Using a National Health Screening Cohort
Authors: Park Sung Joon , Jung Hahn Jin , Park Min Woo , Choi Hyo Geun , Kim Heejin , Wee Jee Hye
Published: June 2024

Tonsillectomy has been suggested as a potential intervention to resolve psoriasis; however, its preventive effects on the development of psoriasis remain unclear. This study aimed to investigate the risk of developing late-onset psoriasis among a Korean adult population who had undergone tonsillectomy. Data from the Korean National Health Insurance Service-Health Screening Cohort between 2002 and 2019 were utilized. Out of a total of 514,866 participants, 1082 participants aged 40 years or older who had undergone tonsillectomy were matched with 4328 control participants using overlap weighting adjustment based on the propensity score. The incidence and hazard ratio (HR) of psoriasis were calculated for both tonsillectomy and control groups. The incidence rates of psoriasis were 1.30% in the tonsillectomy group and 1.20% in the control group. The incidence of psoriasis (overlap-weighted HR = 1.08, 95% confidence of interval = 0.69–1.69, and p = 0.732) did not differ significantly between the patients who underwent tonsillectomy and those in the control group. The cumulative probability of developing psoriasis was not different between the two groups (Log-rank test: p = 0.440). These findings were consistent across subgroups divided by age, sex, income, and region of residence. We found that tonsillectomy did not confer a preventive effect on the development of late-onset psoriasis in the Korean adult population.
Correction: Chaparro et al. Whey as an Alternative Nutrient Medium for Growth of Sporosarcina pasteurii and Its Effect on CaCO3 Polymorphism and Fly Ash Bioconsolidation. Materials 2021, 14, 2470
Authors: Chaparro Sandra , Rojas Hugo A. , Caicedo Gerardo , Romanelli Gustavo , Pineda Antonio , Luque Rafael , Martínez José J.
Published: June 2024

The Editorial Office was made aware of an error in Figure S1 within the original publication [...]
Evaluating the Impact of Oleocanthal and Oleacein on Skin Aging: Results of a Randomized Clinical Study
Authors: Nisticò Steven P. , Greco M. E. , Amato S. , Bennardo L. , Zappia E. , Pignataro E. , Pellacani G.
Published: June 2024

The prevalence of skin aging and the request for effective treatments have driven dermatological research towards natural solutions. This study investigates the anti-aging efficacy of two bioactive natural polyphenols, Oleocanthal and Oleacein, in a skincare formulation. A single-blind, randomized clinical trial involved 70 participants, using a comprehensive exclusion criterion to ensure participant safety and study integrity. Participants applied the Oleocanthal and Oleacein 1% serum formulation twice daily for 30 days. The efficacy was objectively assessed using the VISIA® Skin Analysis System at baseline, after 15 days, and after 30 days. Results indicated significant wrinkle reduction in most groups. For women aged 45–79 years, the mean change was −33.91% (95% CI: −46.75% to −21.07%). For men aged 20–44 years, it was −51.93% (95% CI: −76.54% to −27.33%), and for men aged 45–79 years, it was −46.56% (95% CI: −58.32% to −34.81%). For women aged 20–44 years, the change was −25.68% (95% CI: −63.91% to 12.54%), not statistically significant. These findings highlight the potential of EVOO-derived polyphenols in anti-aging skincare, particularly for older adults. This research paves the way for further exploration into natural compounds in dermatology, particularly for aging skin management.
Impact of Sub-Ambient Temperature on Aging Rate and Gas Separation Properties of Polymers of Intrinsic Microporosity
Authors: Dieudonné Pierre , Rea Riccardo , Lasseuguette Elsa , Ferrari Maria-Chiara
Published: June 2024

Aging in polymers of intrinsic microporosity has slowed exploitation due to a decay in performance over time since densification makes them unsuitable for industrial applications. This work aimed to study the impact of the operation and storage temperature on the gas separation properties and aging rates of PIM-1 self-standing films. The permeability, diffusivity, and solubility of the tested membranes were monitored through permeation tests for pure carbon dioxide and nitrogen at a maximum upstream pressure of 1.3 bar for temperatures ranging from −20 °C to 25 °C. This study found significant benefits in the operation of glassy polymeric membranes at low temperatures, resulting in a favourable trade-off in separation performance and a reduction in the aging rate by three orders of magnitude. This brings new opportunities for the industrial application of PIMs in innovative carbon capture processes.
UPLC-ESI-MS/MS Profiling of Secondary Metabolites from Methanol Extracts of In Vivo and In Vitro Tissues of Daucus capillifolius Gilli (A Comparative Study)
Authors: Abdallah Rehab H. , Hassan Wafaa H. B. , Al-Massarani Shaza M. , Abdel-Mageed Wael M. , Eldahmy Samih I. , Basudan Omer A. , Parveen Mehtab , El Senosy Entesar , Abdelaziz Sahar
Published: June 2024

Daucus capillifolius Gilli is a rare annual wild herb grown in Libya. It belongs to the Apiaceae family, which is one of the largest flowering plant families. Plants of this family are outstanding sources of various secondary metabolites with various biological activities. A UPLC-ESI-MS/MS analysis of different extracts of in vivo and in vitro tissues of Daucus capillifolius together with the fruit extract of the cultivated plant in both ionization modes was carried out for the first time in the current study. Our results reveal the tentative identification of eighty-seven compounds in the tested extracts, including thirty-two phenolic acids and their derivatives; thirty-seven flavonoid glycosides and aglycones of apigenin, luteolin, diosmetin, myricetin and quercetin, containing glucose, rhamnose, pentose and/or glucuronic acid molecules; seven anthocyanins; six tannins; three acetylenic compounds; and three nitrogenous compounds. The tentative identification of the above compounds was based on the comparison of their retention times and ESI-MS/MS fragmentation patterns with those previously reported in the literature. For this Apiaceae plant, our results confirm the presence of a wide array of secondary metabolites with reported biological activities. This study is among the first ones to shed light on the phytoconstituents of this rare plant.
Pickering Emulsion of Oleoresin from Dipterocarpus alatus Roxb. ex G. Don and Its Antiproliferation in Colon (HCT116) and Liver (HepG2) Cancer Cells
Authors: Pocasap Piman , Tamprasit Kawintra , Rungsri Thanyathanya , Kaimuangpak Karnchanok , Srisongkram Tarapong , Katekaew Somporn , Kamwilaisak Khanita , Puthongking Ploenthip , Weerapreeyakul Natthida
Published: June 2024

Oleoresin of Dipterocarpus alatus Roxb. ex G. Don (DA) has been traditionally used for local medicinal applications. Several in vitro studies have indicated its pharmacological potential. However, the low water solubility hinders its use and development for pharmaceutical purposes. The study aimed to (1) formulate oil-in-water (o/w) Pickering emulsions of DA oleoresin and (2) demonstrate its activities in cancer cells. The Pickering emulsions were formulated using biocompatible carboxylated cellulose nanocrystal (cCNC) as an emulsifier. The optimized emulsion comprised 3% (F1) and 4% (v/v) (F2) of oleoresin in 1% cCNC and 0.1 M NaCl, which possessed homogeneity and physical stability compared with other formulations with uniform droplet size and low viscosity. The constituent analysis indicated the presence of the biomarker dipterocarpol in both F1 and F2. The pharmacological effects of the two emulsions were demonstrated in vitro against two cancer cell lines, HepG2 and HCT116. Both F1 and F2 suppressed cancer cell viability. The treated cells underwent apoptosis, as demonstrated by distinct nuclear morphological changes in DAPI-stained cells and Annexin V/PI-stained cells detected by flow cytometry. Our study highlights the prospect of Pickering emulsions for oleoresin, emphasizing enhanced stability and potential pharmacological advantages.
Molecular Insight into the Processes and Mechanisms of N2 Adsorption and Accumulation at the Hydrophobic Solid/Liquid Interface
Authors: Li Bao , Su Dan
Published: June 2024

In this study, molecular dynamics (MD) simulations were employed to elucidate the processes and underlying mechanisms that govern the adsorption and accumulation of gas (represented by N2) at the hydrophobic solid–liquid interface, using the GROMACS program with an AMBER force field. Our findings indicate that, regardless of surface roughness, the presence of water molecules is a prerequisite for the adsorption and aggregation of N2 molecules on solid surfaces. N2 molecules dissolved in water can cluster even without a solid substrate. In the gas–solid–liquid system, the exclusion of water molecules at the hydrophobic solid–liquid interface and the adsorption of N2 molecules do not occur simultaneously. A loosely arranged layer of water molecules is initially formed on the hydrophobic solid surface. The two-stage process of N2 molecule adsorption and accumulation at the hydrophobic solid/liquid interface involves initial adsorption to the solid surface, displacing water molecules, followed by N2 accumulation via self-interaction after saturating the substrate’s surface. The process and underlying mechanisms of gas adsorption and accumulation at hydrophobic solid/liquid interfaces elucidated in this study offer a molecular-level understanding of nano-gas layer formation.
Multimodal Prehabilitation for Patients with Crohn’s Disease Scheduled for Major Surgery: A Narrative Review
Authors: Fiorindi Camilla , Giudici Francesco , Testa Giuseppe Dario , Foti Lorenzo , Romanazzo Sara , Tognozzi Cristina , Mansueto Giovanni , Scaringi Stefano , Cuffaro Francesca , Nannoni Anita , Soop Mattias , Baldini Gabriele
Published: June 2024

Approximately 15–50% of patients with Crohn’s disease (CD) will require surgery within ten years following the diagnosis. The management of modifiable risk factors before surgery is essential to reduce postoperative complications and to promote a better postoperative recovery. Preoperative malnutrition reduced functional capacity, sarcopenia, immunosuppressive medications, anemia, and psychological distress are frequently present in CD patients. Multimodal prehabilitation consists of nutritional, functional, medical, and psychological interventions implemented before surgery, aiming at optimizing preoperative status and improve postoperative recovery. Currently, studies evaluating the effect of multimodal prehabilitation on postoperative outcomes specifically in CD are lacking. Some studies have investigated the effect of a single prehabilitation intervention, of which nutritional optimization is the most investigated. The aim of this narrative review is to present the physiologic rationale supporting multimodal surgical prehabilitation in CD patients waiting for surgery, and to describe its main components to facilitate their adoption in the preoperative standard of care.
Exploring the Impact of Extra Virgin Olive Oil on Maternal Immune System and Breast Milk Composition in Rats
Authors: Zhan-Dai Sonia , Grases-Pintó Blanca , Lamuela-Raventós Rosa M. , Castell Margarida , Pérez-Cano Francisco J. , Vallverdú-Queralt Anna , Rodríguez-Lagunas Maria José
Published: June 2024

Maternal breast milk plays a key role in providing newborns with passive immunity and stimulating the maturation of an infant’s immune system, protecting them from many diseases. It is known that diet can influence the immune system of lactating mothers and the composition of their breast milk. The aim of this study was to establish if a supplementation during the gestation and lactation of Lewis rats with extra virgin olive oil (EVOO), due to the high proportion of antioxidant components in its composition, has an impact on the mother’s immune system and on the breast milk’s immune composition. For this, 10 mL/kg of either EVOO, refined oil (control oil) or water (REF group) were orally administered once a day to rats during gestation and lactation periods. Immunoglobulin (Ig) concentrations and gene expressions of immune molecules were quantified in several compartments of the mothers. The EVOO group showed higher IgA levels in both the breast milk and the mammary glands than the REF group. In addition, the gene expression of IgA in mammary glands was also boosted by EVOO consumption. Overall, EVOO supplementation during gestation and lactation is safe and does not negatively affect the mother’s immune system while improving breast milk immune composition by increasing the presence of IgA, which could be critical for an offspring’s immune health.
Heparin Dosing Regimen Optimization in Veno-Arterial Extracorporeal Membrane Oxygenation: A Pharmacokinetic Analysis
Authors: Lanoiselée Julien , Mourer Jérémy , Jungling Marie , Molliex Serge , Thellier Lise , Tabareau Julien , Jeanpierre Emmanuelle , Robin Emmanuel , Susen Sophie , Tavernier Benoit , Vincentelli André , Ollier Edouard , Moussa Mouhamed Djahoum
Published: June 2024

Background. Unfractionated heparin is administered in patients undergoing veno-arterial extracorporeal membrane oxygenation (VA-ECMO). Anticoagulation monitoring is recommended, with an anti-activated factor X (anti-Xa) targeting 0.3 to 0.7 IU/mL. Owing to heparin’s heterogeneous pharmacokinetic properties, anti-Xa is unpredictable, generating a challenge in anticoagulation practices. The aim of this study was to build a pharmacokinetic model of heparin accounting for potential confounders, and derive an optimized dosing regimen for a given anti-Xa target. Methods. Adult patients undergoing VA-ECMO were included between January 2020 and June 2021. Anticoagulation was managed with an initial 100 IU/kg heparin loading dose followed by a continuous infusion targeting 0.2 to 0.7 IU/mL anti-Xa. The data were split into model development and model validation cohorts. Statistical analysis was performed using a nonlinear mixed effects modeling population approach. Model-based simulations were performed to develop an optimized dosing regimen targeting the desired anti-Xa. Results. A total of 74 patients were included, with 1703 anti-Xa observations. A single-compartment model best fitted the data. Interpatient variability for distribution volume was best explained by body weight, C-reactive protein and ECMO indication (post-cardiotomy shock or medical cardiogenic shock), and interpatient variability for elimination clearance was best explained by serum creatinine and C-reactive protein. Simulations using the optimized regimen according to these covariates showed accurate anti-Xa target attainment. Conclusion. In adult patients on VA-ECMO, heparin’s effect increased with serum creatinine and medical indication, whereas it decreased with body weight and systemic inflammation. We propose an optimized dosing regimen accounting for key covariates, capable of accurately predicting a given anti-Xa target.
Fake News: “No Ban, No Spread—With Sequestration”
Authors: Galam Serge
Published: June 2024

To curb the spread of fake news, I propose an alternative to the current trend of implementing coercive measures. This approach would preserve freedom of speech while neutralizing the social impact of fake news. The proposal relies on creating an environment to naturally sequestrate fake news within quite small networks of people. I illustrate the process using a stylized model of opinion dynamics. In particular, I explore the effect of a simultaneous activation of prejudice tie breaking and contrarian behavior, on the spread of fake news. The results show that indeed most pieces of fake news do not propagate beyond quite small groups of people and thus pose no global threat. However, some peculiar sets of parameters are found to boost fake news so that it “naturally” invades an entire community with no resistance, even if initially shared by only a handful of agents. These findings identify the modifications of the parameters required to reverse the boosting effect into a sequestration effect by an appropriate reshaping of the social geometry of the opinion dynamics landscape. Then, all fake news items become “naturally” trapped inside limited networks of people. No prohibition is required. The next significant challenge is implementing this groundbreaking scheme within social media.