bluuebunny/crossref_metadata_2025 huggingface dataset:
Building Thermal-Network Models: A Comparative Analysis, Recommendations, and Perspectives
Authors: Boodi Abhinandana , Beddiar Karim , Amirat Yassine , Benbouzid Mohamed
Published: February 2022

The development of smart buildings, as well as the great need for energy demand reduction, has renewed interest in building energy demand prediction. Intelligent controllers are a solution for optimizing building energy consumption while maintaining indoor comfort. The controller efficiency on the other hand, is mainly determined by the prediction of thermal behavior from building models. Due to the development complexity of the models, these intelligent controllers are not yet implemented on an industrial scale. There are primarily three types of building models studied in the literature: white-box, black-box, and gray-box. The gray-box models are found to be robust, efficient, of low cost computationally, and of moderate modeling complexity. Furthermore, there is no standard model configuration, development method, or operation conditions. These parameters have a significant influence on the model performance accuracy. This motivates the need for this review paper, in which we examined various gray-box models, their configurations, parametric identification techniques, and influential parameters.
A Study on the Possibility of Using Iron Scale in the Construction of Electromagnetic Field Shields
Authors: Jakubas Adam , Łada-Tondyra Ewa , Makówka Marcin , Suchecki Łukasz
Published: February 2022

Electromagnetic radiation can have a negative effect on electronic circuits, for example, by disrupting their work and communication. Therefore, there is a need to reduce or eliminate electromagnetic interference using shields. This paper focuses on using waste materials in the form of iron scale, which is easy and inexpensive to obtain for shielding. Iron scale is the result of gaseous or aqueous corrosion, due to which a superficial layer of metal oxides formed on the surface of heated metal objects as a result of their contact with air or water during an industrial process is developed. In this study, morphology, chemical, and phase composition measurements were carried out. The results show that all the iron-based materials investigated are mainly composed of iron oxides and pure iron. The polymer–iron composites with different quantitative and qualitative compositions were prepared to verify the shielding effectiveness. The electrical resistance, impact strength, and magnetic properties were measured, and dynamic mechanical thermal analysis and tensile strength tests were performed. The tests confirmed the suitability of iron scale for the construction of electromagnetic field shielding material. Iron scale is cheap and easy to obtain from iron-based waste materials. The developed composites are easy to form and use recycled material.
Proficiency Level of Leadership Competences on the Initial Training Course for Firefighters—A Case Study of Lisbon Fire Service
Authors: Carolino João , Rouco Carlos
Published: February 2022

Leadership competence development in the Portuguese fire services is an area to be explored and one where we must obtain more profound knowledge. The research seeks to identify the role of the initial training course for professional firefighters in the proficiency level of leadership competences in trainees at the Lisbon Fire Service. To achieve the objectives of the investigation, the researchers adopted a quantitative method with a longitudinal case study research design, using a questionnaire of leadership competences, applied to 126 trainees of the Initial Training Course for professional firefighters from Lisbon Fire Service (case study), at three different moments during a one-year training period (longitudinal study). From the obtained results, it was concluded that the leadership competences with the highest level of proficiency at the end of the course are problem solving, participatory leadership, delegative leadership, conflict management, influence by example, task orientation, decision making, vision and proactivity.
Trawler Engine Size Had No Effect on Baltic Herring Size/Age Structure: An Experimental Study in the Gulf of Riga, Baltic Sea
Authors: Sepp Elor , Vetemaa Markus , Raid Tiit , Arula Timo
Published: February 2022

The Gulf of Riga stock of Baltic herring (Clupea harengus membras L.) has been maintained through several management tools. One such tool is the restriction of vessels’ main engine power (<221 kW). This restriction was implemented in the early 1990s and is based on the vessel types available in the area and on the assumption that the gear size used in trawl fishery depends on the vessel size (power). In the current study, we compared vessels with different engine powers using the same gears currently allowed in the gulf, to identify whether vessel power had any relation to catch structure. The results showed that engine power did not explain the differences in catch structure, which were more dependent on season and depth of water. Easing the power restriction of the trawl vessels in the Gulf of Riga will most likely not have a major negative impact on the sustainable management of the herring population. However, vessels with higher engine power should not use larger trawl gear than is currently used in the gulf.
The Influence of Hypothermia Hibernation Combined with CO2 Anesthesia on Life and Storage Quality of Large Yellow Croaker (Pseudosciaena crocea)
Authors: Tan Nanfeng , Gao Yuanpei , Wang Yueke , Deng Shanggui , Yuan Pengxiang , Jiang Tong , Zheng Wanyuan
Published: February 2022

We explore the feasibility of the long-term transportation of live large yellow croakers (Pseudosciaena crocea) using the combined method of CO2 anesthesia and hypothermia hibernation, and its effect on the quality of recovered fish stored at 4 °C. Fish treated with CO2 anesthesia at a 2 ppm/s aeration rate were cooled at 3 °C/h to hibernate survived for 36 h at 8 °C in seawater. This method resulted in better survival rates and time, and a lower operational time than hypothermia hibernation or CO2 anesthesia methods. The results of a blood analysis indicated that the stress experienced by the fish during hibernation was mitigated, but existent after recovery. The drip loss rate of the ordinary muscle of hibernated fish was significantly different from that of the control group at 4 °C, but there was no significant difference in the pH, lactic acid content, and color during early storage. Furthermore, hibernation did not affect springiness and chewiness. Thus, the combination of CO2 anesthesia and hibernation may improve the survival and operation efficiency of fish in long-term transportation. However, this method affects the quality of fish after long-term storage. Thus, hibernated fish should be consumed after appropriate domestication or immediately after recovery.
Vacuum Packaging Can Extend Fresh Color Characteristics of Beef Steaks during Simulated Display Conditions
Authors: Reyes Tristan M. , Wagoner Madison P. , Zorn Virginia E. , Coursen Madison M. , Wilborn Barney S. , Bonner Tom , Brandebourg Terry D. , Rodning Soren P. , Sawyer Jason T.
Published: February 2022

Packaging technology is evolving, and the objectives of this study were to evaluate instrumental surface color, expert color evaluation, and lipid oxidation (TBARS) on beef longissimus lumborum steaks packaged in vacuum-ready packaging (VRF) or polyvinyl chloride (PVC) overwrap packaging. Paired strip loins (Institutional Meat Purchasing Specifications # 180) were cut into 2.54-cm-thick steaks and assigned randomly to one of two packaging treatments, VRF or PVC. Steaks packaged in VRF were lighter in color (p < 0.05) as the display period increased, whereas steaks packaged in PVC became darker (p < 0.05). Redness (a*) values were greater (p < 0.05) for PVC steaks until day 5, whereas VRF steaks had a greater (p < 0.05) surface redness from day 10 to 35 of the display period. Calculated spectral values of red to brown were greater (p < 0.05) for steaks in VRF than PVC. In addition, expert color evaluators confirmed VRF steaks were less brown and less discolored (p < 0.05) from day 5 to 35 of the display. Nonetheless, lipid oxidation was greater (p < 0.05) for PVC steaks from day 10 through day 35 of the display. Results from this study suggest that the use of vacuum packaging for beef steaks is plausible for maintaining surface color characteristics during extended display periods.
Process Development for the Instant Quantification of Lycopene from Agricultural Produces Using Supercritical Fluid Chromatography-Diode Array Detector (SFC-DAD)
Authors: Priyadarsani Supriya , Patel Avinash Singh , Sharma Yogesh , Kar Abhijit
Published: February 2022

A quick, simple, and reliable isocratic ultra-performance supercritical fluid chromatography-photodiode array detector (UPSFC-DAD) method was developed and validated to determine lycopene in different horticultural products. The effects of stationary phase, co-solvent, pressure, temperature, flow rate, and mobile phase additive on the separation of lycopene were evaluated. The developed method involved BEH-2EP—2.1 × 150 mm, 5 µm as the stationary phase, and CO2/MeOH 85:15 (v/v) with formic acid as the additive at 0.10% as the mobile phase. The column temperature was maintained at 45 °C, ABPR at 1800 psi, and the mobile phase’s flow rate was maintained at 1 mL/min. Under the optimized conditions, lycopene was successfully separated within 0.722 ± 0.001 min. The standard curve assayed over a range of 10 to 100 µg/mL resulted in a correlation coefficient of 0.998. The mean recoveries between 97.38% and 102.67% at different spiking levels with RSD < 2.5% were achieved. The intra and inter-day precision expressed as relative standard deviations (RSD) were found to range from 1.27% to 3.28% and from 1.57% to 4.18%, respectively. Robustness in terms of retention time (tR) and RSD were found to be 0.93 ± 0.23 min and less <2.80%, respectively. The limits of detection and quantification were 0.14 µg/mL and 0.37 µg/mL, respectively. This method was successfully applied to determine lycopene extracted from papaya, grapefruit, and bitter melon.
Transcriptome Analysis of Halotolerant Staphylococcus saprophyticus Isolated from Korean Fermented Shrimp
Authors: Jo Eunhye , Hwang Sungmin , Cha Jaeho
Published: February 2022

Saeu-jeotgal, a Korean fermented shrimp food, is commonly used as an ingredient for making kimchi and other side dishes. The high salinity of the jeotgal contributes to its flavor and inhibits the growth of food spoilage microorganisms. Interestingly, Staphylococcus saprophyticus was discovered to be capable of growth even after treatment with 20% NaCl. To elucidate the tolerance mechanism, a genome-wide gene expression of S. saprophyticus against 0%, 10%, and 20% NaCl was investigated by RNA sequencing. A total of 831, 1314, and 1028 differentially expressed genes (DEGs) were identified in the 0% vs. 10%, 0% vs. 20%, and 10% vs. 20% NaCl comparisons, respectively. The Clusters of Orthologous Groups analysis revealed that the DEGs were involved in amino acid transport and metabolism, transcription, and inorganic ion transport and metabolism. The functional enrichment analysis showed that the expression of the genes encoding mechanosensitive ion channels, sodium/proton antiporters, and betaine/carnitine/choline transporter family proteins was downregulated, whereas the expression of the genes encoding universal stress proteins and enzymes for glutamate, glycine, and alanine synthesis was upregulated. Therefore, these findings suggest that the S. saprophyticus isolated from the saeu-jeotgal utilizes different molecular strategies for halotolerance, with glutamate as the key molecule.
Validation of the Magnetic Stirrer Method for the Detection of Trichinella Larvae in Muscle Samples Based on Proficiency Tests Results
Authors: Różycki Mirosław , Korpysa-Dzirba Weronika , Bełcik Aneta , Bilska-Zając Ewa , Kochanowski Maciej , Karamon Jacek , Sroka Jacek , Cencek Tomasz
Published: February 2022

Trichinellosis is a zoonotic disease caused by the nematodes of the genus Trichinella. Infection takes place through the consumption of infected meat containing live larvae. The only way to prevent the disease is to break its epizootic chain. To ensure effective control of Trichinella spp., a range of preventive and control measures have been undertaken. These efforts have been focused on controlling Trichinella in domestic pigs, the main source of the disease. Artificial digestion is also the reference point for other methods for Trichinella risk control. Descriptive data validation of the digestion assay was presented in 1998 based on results published by scientific laboratories. Herein, we supplement those data by characterizing the method’s performance in inter-laboratory comparisons. The source of data was the results of Proficiency Testing conducted in 2015–2019. Samples were contaminated by 0, 1, 3, and 5 larvae. In total, 7580 samples were examined by the laboratories. Based on Proficiency Testing results, the main parameters characterizing the method performance in field conditions were established as follows: specificity, 97.3%; sensitivity, 86.5%; accuracy, 89.2%; uncertainty, 0.3; limit of detection (LOD), 1 larva; and limit of quantification (LOQ), 3 larvae.
Identification of β-Glucosidase Activity of Lentilactobacillus buchneri URN103L and Its Potential to Convert Ginsenoside Rb1 from Panax ginseng
Authors: Renchinkhand Gereltuya , Magsar Urgamal , Bae Hyoung Churl , Choi Suk-Ho , Nam Myoung Soo
Published: February 2022

Lentilactobacillus buchneri isolated from Korean fermented plant foods produces β-glucosidase, which can hydrolyze ginsenoside Rb1 from Panax ginseng to yield ginsenoside Rd. The aim of this study was to determine the mechanisms underlying the extracellular β-glucosidase activity obtained from Lentilactobacillus buchneri URN103L. Among the 17 types of lactic acid bacteria showing positive β-glucosidase activity in the esculin iron agar test, only URN103L was found to exhibit high hydrolytic activity on ginsenoside Rb1. The strain showed 99% homology with Lentilactobacillus buchneri NRRLB 30929, whereby it was named Lentilactobacillus buchneri URN103L. Supernatants of selected cultures with β-glucosidase activity were examined for hydrolysis of the major ginsenoside Rb1 at 40 °C, pH 5.0. Furthermore, the β-glucosidase activity of this strain showed a distinct ability to hydrolyze major ginsenoside Rb1 into minor ginsenosides Rd and Rg3. Lentilactobacillus buchneri URN103L showed higher leucine arylamidase, valine arylamidase, α-galactosidass, β–galactosidase, and β-glucosidase activities than any other strain. We conclude that β-glucosidase from Lentilactobacillus buchneri URN103L can effectively hydrolyze ginsenoside Rb1 into Rd and Rg3. The converted ginsenoside can be used in functional foods, yogurts, beverage products, cosmetics, and other health products.
Vacuum-Packed Steak Tartare: Prevalence of Listeria monocytogenes and Evaluation of Efficacy of ListexTM P100
Authors: Hluchanova Lucie , Korena Kristyna , Juricova Helena
Published: February 2022

Steak tartare is a raw, ready-to-eat meal popular in European countries, the safety of which is often discussed due to the risk of foodborne illness. The aim of this study was to determine the prevalence of Listeria monocytogenes in vacuum-packed steak tartare from retailers in the Czech Republic, characterize the strains obtained by typing methods and to evaluate the efficacy of ListexTM P100 against L. monocytogenes artificially inoculated into steak tartare samples. The prevalence of L. monocytogenes was 55% and 17 isolates belonging mostly to serotype 1/2a were obtained. Altogether 11 sequence types and 11 clonal complexes were assigned based on the whole genome sequencing (WGS) signifying the high diversity of L. monocytogenes isolates obtained. Core genome multi-locus sequence typing (cgMLST) did not confirm an epidemiological connection with human cases of listeriosis. The efficacy of ListexTM P100 treatment at concentrations of 108 and 109 PFU/g on artificially inoculated beef steak tartare samples was not efficient. Based on the results of this study, steak tartare from retailers can be considered as a source of L. monocytogenes that remains a challenge to the food industry.
High-Performance Thin-Layer Chromatography-Immunostaining as a Technique for the Characterization of Whey Protein Enrichment in Edam Cheese
Authors: Treblin Mascha , von Oesen Tobias , Lüneburg Jana , Clawin-Rädecker Ingrid , Martin Dierk , Schrader Katrin , Zink Ralf , Hoffmann Wolfgang , Fritsche Jan , Rohn Sascha
Published: February 2022

Whey protein-enriched cheese can be produced by means of a high-temperature treatment of a part of the cheese milk. In this way, the nutritional quality of the resulting cheeses can be increased while resources are conserved. High-performance thin-layer chromatography-immunostaining (HPTLC-IS) using specific β-lactoglobulin (β-LG) antibodies was applied to study the implementation and stability of β-LG in two different sample sets of whey protein-enriched Edam model cheeses, including industrial-scale ones. Two methods were compared for the extraction of the proteins/peptides from the cheese samples. By applying tryptic hydrolysis directly from a suspended cheese sample instead of a supernatant of a centrifuged suspension, a better yield was obtained for the extraction of β-LG. When applying this method, it was found that selected epitopes in the tryptic β-LG peptides remain stable over the ripening period of the cheese. For four of the tryptic β-LG peptides detected by immunostaining, the amino acid sequence was identified using MALDI-TOF-MS/MS. One of the peptides identified was the semi-tryptic peptide VYVEELKPTP. A linear relationship was found between the content of this peptide in cheese and the proportion of high-heated milk in the cheese milk. β-LG enrichment factors of 1.72 (n = 3, sample set I) and 1.33 ± 0.19 (n = 1, sample set II) were determined for the cheese samples containing 30% high-heated milk compared to the non-enriched samples. The relative β-LG contents in the cheese samples with 30% high-heated milk were calculated to be 4.35% ± 0.39% (sample set I) and 9.11% ± 0.29% (sample set II) using a one-point calibration. It can be concluded that the HPTLC-IS method used is a suitable tool for the analysis of whey protein accumulation in cheese, being therefore potentially directly applicable on an industrial scale. For more accurate quantification of the whey protein content in cheese, an enhanced calibration curve needs to be applied.
Improving the Quality of Frozen Lamb by Microencapsulated Apple Polyphenols: Effects on Cathepsin Activity, Texture, and Protein Oxidation Stability
Authors: Zhong Yuanyuan , Liu Yangming , Xing Lijie , Zhao Mou , Wu Wenxia , Wang Qingling , Ji Hua , Dong Juan
Published: February 2022

This study aimed to evaluate the efficiency of microencapsulated apple polyphenols (MAP) in controlling cathepsin activity and texture, as well as inhibiting protein oxidation and metmyoglobin formation in lamb meat during frozen storage at −18 °C for 40 weeks. The effects of degradation in vitro on cathepsin and the microstructure in lamb were also evaluated. Results indicated that relative to the control group, the lamb treated with MAP exhibited increased cathepsin activity and inhibited metmyoglobin production. Textural characteristics, such as hardness and springiness, significantly changed (p < 0.05). Treatment with 0.2–1.6 mg/mL of MAP effectively reduced the mean particle size, increasing the zeta potential, delaying the conversion of α-helices to random coils, and maintaining the integrity of the tissue structure. However, treatment with 3.2 mg/mL of MAP damaged the protein structure. Degradation in vitro indicated that protein oxidation hindered the effect of cathepsin and was a dominant factor affecting protein during the frozen storage. These results demonstrated that microencapsulation can potentially be used for meat preservation and replace chemical antioxidants in the meat industry.
The Multi-Switching Sliding Mode Combination Synchronization of Fractional Order Non-Identical Chaotic System with Stochastic Disturbances and Unknown Parameters
Authors: Pan Weiqiu , Li Tianzeng , Wang Yu
Published: February 2022

This paper deals with the issue of the multi-switching sliding mode combination synchronization (MSSMCS) of fractional order (FO) chaotic systems with different structures and unknown parameters under double stochastic disturbances (SD) utilizing the multi-switching synchronization method. The stochastic disturbances are considered as nonlinear uncertainties and external disturbances. Our theoretical part considers that the drive-response systems have the same or different dimensions. Firstly, a FO sliding surface is established in terms of the fractional calculus. Secondly, depending on the FO Lyapunov stability theory and the sliding mode control technique, the multi-switching adaptive controllers (MSAC) and some suitable multi-switching adaptive updating laws (MSAUL) are designed. They can ensure that the state variables of the drive systems are synchronized with the different state variables of the response systems. Simultaneously, the unknown parameters are assessed, and the upper bound values of stochastic disturbances are examined. Selecting the suitable scale matrices, the multi-switching projection synchronization, multi-switching complete synchronization, and multi-switching anti-synchronization will become special cases of MSSMCS. Finally, examples are displayed to certify the usefulness and validity of the scheme via MATLAB.
Fractional p(·)-Kirchhoff Type Problems Involving Variable Exponent Logarithmic Nonlinearity
Authors: Zuo Jiabin , Soni Amita , Choudhuri Debajyoti
Published: February 2022

In this paper, we investigate a fractional p(·)-Kirchhoff type problem involving variable exponent logarithmic nonlinearity. With the help of the Nehari manifold approach, the existence and multiplicity of nontrivial weak solutions for the above problem are obtained. The main aspect and challenges of this paper are the presence of double non-local terms and logarithmic nonlinearity.
Some Fixed-Disc Results in Double Controlled Quasi-Metric Type Spaces
Authors: Mlaiki Nabil , Taş Nihal , Haque Salma , Rizk Doaa
Published: February 2022

In this paper, we introduce new types of general contractions for self mapping on double controlled quasi-metric type spaces, where we prove the existence and uniqueness of fixed disc and circle for such mappings.
Analysis of Lie Symmetries with Conservation Laws and Solutions of Generalized (4 + 1)-Dimensional Time-Fractional Fokas Equation
Authors: Jiang Zhuo , Zhang Zong-Guo , Li Jing-Jing , Yang Hong-Wei
Published: February 2022

High-dimensional fractional equations research is a cutting-edge field with significant practical and theoretical implications in mathematics, physics, biological fluid mechanics, and other fields. Firstly, in this paper, the (4 + 1)-dimensional time-fractional Fokas equation in a higher-dimensional integrable system is studied by using semi-inverse and fractional variational theory. Then, the Lie symmetry analysis and conservation law analysis are carried out for the higher dimensional fractional order model with the symmetry of fractional order. Finally, the fractional-order equation is solved using the bilinear approach to produce the rogue wave and multi-soliton solutions, and the fractional equation is numerically solved using the Radial Basis Functions (RBFs) method.
The Application of Focused Medium-Energy Extracorporeal Shockwave Therapy in Hemophilic A Arthropathy
Authors: Lo Wan-Shan , Sheen Jiunn-Ming , Chen Yu-Chieh , Wu Kuan-Ting , Wang Lin-Yi , Lau Yiu-Chung , Hsiao Chih-Cheng , Ko Jih-Yang
Published: February 2022

Hemophilic arthropathy causes the damage of synovium, cartilage, and subchondral bone. The present study evaluated the safety and the effect of extracorporeal shockwave therapy (ESWT), a safe treatment widely used in musculoskeletal conditions in patients with hemophilic arthropathy. Between 1 August 2019 and 31 July 2020, seven hemophilia A patients were enrolled and treated with medium-energy ESWT on the knee joint in the first two months after prophylactic coagulation factor administration. At the beginning of the study and at 1-, 2-, 3-, and 6-month follow-ups, the Hemophilia Joint Health Score (HJHS), visual analog scale score (VAS), and Hemophilia Early Arthropathy Detection with Ultrasound score (HEAD-US) were evaluated for therapeutic effectiveness and safety, while serum bone morphogenetic protein 2 (BMP-2) and von Willebrand factor (vWF) levels were analyzed for assessing chondroprotection and bone healing. Magnetic resonance imaging (MRI) of the knee was performed at the beginning of the study and the 6-month follow-ups. As a result, a non-significant decrease in VAS scores (p = 0.151) but not HJHS after treatment was noticed. At the 3-month follow-up, there was a non-significant increase in BMP2 levels (p = 0.171) but not vWF. Ultrasonography showed no disease activity score elevation in five patients and no further disease damage in all patients. Repeated MRI examinations in three patients showed no structural progression during the 6-month follow-up. As to adverse events, redness, local heat, and mild swelling were noted in five patients without breakthrough bleeding. We concluded that medium-energy ESWT might be safe for hemophilic arthropathy once prophylactic coagulation factors are administered.
Suicide among Health Care Professionals—An Indian Perspective
Authors: Das Nishant , Khar Prerna , Karia Sagar , Shah Nilesh
Published: February 2022

Suicide is a global phenomenon that claims a person’s life every 40 s. The suicide-mortality rate in India is higher than the worldwide average for health care professionals (HCP). The treatment gap for mental health care is alarming, more than 80% in India which has improved compared to a decade. Among the methods chosen by HCPs for dying by suicide, violent suicide methods are more common. Hanging is the most common means, followed by lethal injection and jumping from a building. Among the medical students and professionals in India, academic stress is the leading cause of suicides, followed by mental illness and harassment. Stressfully long working hours, starvation for long hours, inadequate diet, sleep deprivation, inadequate rest, high levels of personal expectations, knowledge of lethal suicide methods, easy access to potentially fatal drugs, apathy, and fearlessness towards death are some of the contributing factors. Primary preventive measures to minimize suicides in HCPs would be to conduct stress-management workshops at an institutional level, routine mental health check-ups in healthcare institutions, mental-health screening for students enrolling into healthcare courses, and prompt referrals to mental healthcare facilities. In addition, telehealth services or mental health services for medical professionals of India are the need of the hour.
Anticipatory Burden in Adult-Child Caregivers: A Concept Analysis
Authors: She Hangying , Man Yuncheng
Published: February 2022

This study aims to analyze the concept of anticipatory burden in adult-child caregivers. A systematic literature review was performed using four databases, Pubmed, CINAHL, PsycINFO and Medline, with the keywords of “anticipatory burden” and “anticipated burden”. Simplified Wilson’s classic concept analysis modified by Walker and Avant was employed to identify the attributes, antecedents and consequences of anticipatory burden in the adult-child caregivers. Eighteen articles were analyzed. Attributes of anticipatory burden in adult-child caregivers were found to be: (1) subjective burden, (2) anticipation, (3) overestimation, (4) inability, and (5) family relationship. Antecedents were identified as: (1) potential care recipients, (2) caregiving willingness, and (3) a lack of resources. Consequences included: (1) prediction of caregiving willingness, (2) impacts on caregivers’ health, (3) intervention promotion, and (4) behavioral changes. As the adult-child caregiver is one of the main types of family caregivers for the fast-growing aging population, it is important to understand the attributes, antecedents, and consequences of their anticipatory burden. Based on the results of this study, resources such as intervention, policy, and counseling services are recommended to help adult-child caregivers lower their anticipatory burden and get better prepared for providing family care.
Root Knot Nematode Presence and Its Integrated Management in Pomegranate Orchards Located in Indian Arid Areas
Authors: Singh Akath , Kaul Ramesh Kumar , Khapte Pratapsingh Suresh , Jadon Kuldeep Singh , Rouphael Youssef , Basile Boris , Kumar Pradeep
Published: February 2022

Nematodes are a serious problem across pomegranate-growing areas worldwide, but the severity is higher in light sandy soils of arid regions. The present study was carried out to explore the integrated approaches for the control of nematodes in pomegranate. Three different experiments were carried out during 2017–2020 to (a) delineate nematode abundance in major pomegranate areas, (b) screen pomegranate genotypes against nematode, and (c) assess the efficacy of integrated management for the control of root knot nematode in pomegranate. The survey results revealed that none of the pomegranate orchards were found to be free from nematode infestation. Moreover, the majority of the orchards (78%) showed moderate incidence (10.1 to 40%) of infestation. A significant yield reduction (40.2%) and a decrease in fruit size was observed in nematode-affected trees. Pattern of cuticular markings in the perineal area of the mature female confirmed the occurrence of Meloidogyne incognita only in all the surveyed orchard of pomegranate. All the evaluated genotypes and varieties were found susceptible to root knot nematodes, but the severity of the attack varied among them. Hence, more detailed screening is needed on a larger population. Nematode population (number of galls g−1 root) can be minimized significantly with the combined applications of Carbofuran at 20 g + Fluensulfone at 20 g per plant or Neemcake 500 g + Paecilomyceslilacinus at 25 mL + Carbofuran at 20 g + Fluensulfone at 20 g per plant in April and August.
Potential Toxic Effects of Exposure to Titanium Silicon Oxide Nanoparticles in Male Rats
Authors: Ghareeb Shimaa , Ragheb Didair , El-Sheakh Aly , Ashour Mohamed-Bassem Ali
Published: February 2022

Recently, nano titanium silicon oxide (TiSiO4 NPs) has been used in different fields and industries. Very few toxicological data exist for TiSiO4 NPs. In the present study, the potential adverse effects of oral exposure to a single dose of TiSiO4 NPs ≤ 50 nm (250 mg/kg b.w.) in adult male rats were investigated through the assessment of biomarkers for serum biochemical parameters, liver DNA damage, and histopathological examination and determination of Si and Ti in the exposed rat tissues. The results revealed that there were no significant changes in serum total protein, albumin, and triglycerides content, while total cholesterol level was significantly increased 7 days after exposure. TiSiO4 NPs significantly increased superoxide dismutase (SOD), glutathione peroxidase (GPx), acetylcholine esterase (AChE), lactate dehydrogenase (LDH) activity, luteinizing hormone (LH), and follicle-stimulating hormone (FSH) levels in the exposed rat serum, whereas alanine aminotransferase (ALT), aspartate aminotransferase (AST) activity, urea level, immunoglobulins (IgG and IgM) concentrations, progesterone, and testosterone levels were significantly decreased. The liver comet assay indices were significantly increased after 7 days post-exposure. Moreover, histopathological changes and the accumulation of Si and Ti in liver, kidney, spleen, and lung tissues of treated rats were recorded.
Identification of Priority Conservation Areas for Natural Heritage Sites Integrating Landscape Ecological Risks and Ecosystem Services: A Case Study in the Bogda, China
Authors: Wang Tian , Chen Xiaodong , Zheng Xin , Lu Yayan , Han Fang , Yang Zhaoping
Published: February 2022

The conservation of World Natural Heritage Sites has become a global concern. The identification of priority conservation areas can preserve the value of heritage sites while promoting sustainable development, which is important for balancing the conservation and development of heritage sites. This paper proposes an integrated framework for the identification of priority conservation areas for natural heritage sites based on landscape ecological risks (LERs) and ecosystem services (ESs), taking the Bogda heritage site in Xinjiang, China as a case study. The innovative approach combined the natural and cultural elements of natural heritage sites and included the following steps: (1) the LER index, Integrated Valuation of Ecosystem Services and Tradeoffs (InVEST) model and questionnaire method were adopted to assess the LERs and ESs of Bogda heritage sites during 1990–2018; (2) ordered weighted averaging (OWA) was used to identify conservation priorities by weighing LERs and ESs; and (3) the optimal priority conservation area was determined by comparing the conservation efficiencies under different scenarios. The results revealed that the LER, carbon storage (CS), habitat quality (HQ), aesthetic value (AV), and recreational value (RV) showed significant spatiotemporal variation. The most suitable priority conservation area was located at the central forestlands and high-coverage grasslands, with conservation efficiencies of 1.16, 2.91, 1.96, 1.03, and 1.21 for LER, CS, HQ, AV, and RV, respectively. Our study demonstrated that integrating LERs and ESs is a comprehensive and effective approach to identifying conservation priorities for heritage sites. The results can provide decision support for the conservation of the Bogda heritage site and a methodological reference for identifying conservation priorities for natural heritage sites. Furthermore, this study is also an effective application of LERs and ESs in identifying priority conservation areas.
Spatiotemporal Characteristics and Patterns of the COVID-19 Pandemic in China: An Empirical Study Based on 413 Cities or Regions
Authors: Shi Jialu , Wang Xuan , Ci Fuyi , Liu Kai
Published: February 2022

The global economy was stagnant and even regressed since the outbreak of COVID-19. Exploring the spatiotemporal characteristics and patterns of COVID-19 pandemic spread may contribute to more scientific and effective pandemic prevention and control. This paper attempts to investigate the spatiotemporal characteristics in cumulative confirmed COVID-19 cases, mortality, and cure rate in 413 Chinese cities or regions using the data officially disclosed by the government. The results showed that: (1) The pandemic development can be divided into five stages: early stage (sustained growth), early mid-stage (accelerated growth), mid-stage (rapid growth), late mid-stage (slow growth), and late-stage (stable disappearance); (2) the cumulative number of confirmed COVID-19 cases remained constant in Wuhan, whilst the mortality tended to rise faster from the early stage to the late-stage and the cure rate moved from the southeast to the northwest; (3) the three indicators mentioned above showed significant and positive spatial correlation. Moran’s I curve demonstrated an inverted “V” trend in cumulative confirmed COVID-19 cases; the mortality curve was generally flat; the cure rate curve tended to rise. There are apparent differences in the local spatial autocorrelation pattern of the three primary indicators.
Altered Visual Reliance Induced by Stroboscopic Glasses during Postural Control
Authors: Lee Hyunwook , Han Seunguk , Hopkins Jon Ty
Published: February 2022

Little is known about how disrupted vision affects visual reliance during postural control. postural control. Twenty-four physically active adults volunteered to participate in the study. Static postural control was quantified with center of pressure measures during a one-legged balance test with four different visual inputs (eyes-open (EO), high frequency of strobe vision (HSV), low frequency of strobe vision (LSV), and eyes-closed (EC)) and on two different surfaces (firm and foam). Dynamic postural control was calculated by the dynamic postural stability index and the Y-Balance test for three different visual inputs (EO, HSV, and LSV) and the two different surfaces. Romberg ratios (HSV/EO, LSV/EO, and EC/EO) were then calculated and used for statistical analysis to assess visual contribution during postural control. In the results, Romberg ratios were higher when people were on the foam surface than the firm surface in center of pressure total path in medial-lateral and anterior-posterior directions (p < 0.05, both directions). Similarly, Romberg ratios were higher on the foam surface than the firm surface in dynamic stability index in medial-lateral and anterior-posterior directions (p < 0.05, both directions). Stroboscopic glasses could alter visual reliance when the somatosensory system is disturbed by a foam pad during both static and dynamic postural control. Clinicians could use the glasses to manipulate visual reliance during dynamic balance training for patients with musculoskeletal injuries.
The Relationship between Metal Exposure and Chronic Obstructive Pulmonary Disease in the General US Population: NHANES 2015–2016
Authors: Fei Qiaoyuan , Weng Xueqiong , Liu Kun , Liu Shan , Chen Jingmin , Guo Xinrong , Jing Chunxia
Published: February 2022

The effects of metal on pulmonary function are inconsistent, and abnormal distribution of metals can decrease lung function. However, the effects of metals exposure on chronic obstructive pulmonary disease (COPD) are still unclear. This study aims to explore the relationship between metal exposure and COPD risk. Cross-sectional data from the National Health and Nutrition Survey (NHANES) 2015–2016 was analyzed. Inductively coupled plasma dynamic reaction cell mass spectrometry (ICP-DRC-MS) was used to measure the metals concentration in the blood. The multiple linear regression and restricted cubic spline (RCS) were used to analyze the relationship between metals exposure and COPD risk. In this study, 1399 participants were included, of which 107 participants were diagnosed with COPD using self-reported chronic bronchitis, emphysema, and COPD. The second and third tertiles of copper increased the COPD risk by 1.98-fold (95% CI: 1.08–3.62) and 2.43-fold (95% CI: 1.32–4.48) compared with the first tertile, using p = 0.005 for the trend after adjusting for the covariates. RCS showed a positive linear correlation between copper and COPD risk (p = 0.006 for overall association) in all participants. When stratified by sex, the multi-factor analysis showed that the third tertile of copper increased male’s COPD risk by 3.42-fold (95% CI: 1.52–7.76), with p = 0.003 for the trend, and RCS also showed a positive linear correlation (p = 0.013 for overall association). Although RCS showed that selenium can reduce the COPD risk (p = 0.008 for overall association) in males, an association between selenium and COPD was not observed (p > 0.05). Our findings suggest that a high concentration of copper may increase COPD risk in males in the general US population, and more research is needed to explore its possible mechanism of action.
Prediction and Uncertainty Capabilities of Quantile Regression Forests in Estimating Spatial Distribution of Soil Organic Matter
Authors: Nikou Melpomeni , Tziachris Panagiotis
Published: February 2022

One of the core tasks in digital soil mapping (DSM) studies is the estimation of the spatial distribution of different soil variables. In addition, however, assessing the uncertainty of these estimations is equally important, something that a lot of current DSM studies lack. Machine learning (ML) methods are increasingly used in this scientific field, the majority of which do not have intrinsic uncertainty estimation capabilities. A solution to this is the use of specific ML methods that provide advanced prediction capabilities, along with innate uncertainty estimation metrics, like Quantile Regression Forests (QRF). In the current paper, the prediction and the uncertainty capabilities of QRF, Random Forests (RF) and geostatistical methods were assessed. It was confirmed that QRF exhibited outstanding results at predicting soil organic matter (OM) in the study area. In particular, R2 was much higher than the geostatistical methods, signifying that more variation is explained by the specific model. Moreover, its uncertainty capabilities as presented in the uncertainty maps, shows that it can also provide a good estimation of the uncertainty with distinct representation of the local variation in specific parts of the area, something that is considered a significant advantage, especially for decision support purposes.
Bis-Indole Alkaloids Isolated from the Sponge Spongosorites calcicola Disrupt Cell Membranes of MRSA
Authors: Khan Neyaz A. , Kaur Navdeep , Owens Peter , Thomas Olivier P. , Boyd Aoife
Published: February 2022

Antimicrobial resistance (AMR) is a global health challenge with methicillin resistant Staphylococcus aureus (MRSA), a leading cause of nosocomial infection. In the search for novel antibiotics, marine sponges have become model organisms as they produce diverse bioactive compounds. We investigated and compared the antibacterial potential of 3 bis-indole alkaloids—bromodeoxytopsentin, bromotopsentin and spongotine A—isolated from the Northeastern Atlantic sponge Spongosorites calcicola. Antimicrobial activity was determined by MIC and time-kill assays. The mechanism of action of bis-indoles was assessed using bacterial cytological profiling via fluorescence microscopy. Finally, we investigated the ability of bis-indole alkaloids to decrease the cytotoxicity of pathogens upon co-incubation with HeLa cells through the measurement of mammalian cell lysis. The bis-indoles were bactericidal to clinically relevant Gram-positive pathogens including MRSA and to the Gram-negative gastroenteric pathogen Vibrio parahaemolyticus. Furthermore, the alkaloids were synergistic in combination with conventional antibiotics. Antimicrobial activity of the bis-indole alkaloids was due to rapid disruption and permeabilization of the bacterial cell membrane. Significantly, the bis-indoles reduced pathogen cytotoxicity toward mammalian cells, indicating their ability to prevent bacterial virulence. In conclusion, sponge bis-indole alkaloids are membrane-permeabilizing agents that represent good antibiotic candidates because of their potency against Gram-positive and Gram-negative bacterial pathogens.
Remodeling of Lipid A in Pseudomonas syringae pv. phaseolicola In Vitro
Authors: Gerster Tim , Wröbel Michelle , Hofstaedter Casey E. , Schwudke Dominik , Ernst Robert K. , Ranf Stefanie , Gisch Nicolas
Published: February 2022

Pseudomonas species infect a variety of organisms, including mammals and plants. Mammalian pathogens of the Pseudomonas family modify their lipid A during host entry to evade immune responses and to create an effective barrier against different environments, for example by removal of primary acyl chains, addition of phosphoethanolamine (P-EtN) to primary phosphates, and hydroxylation of secondary acyl chains. For Pseudomonas syringae pv. phaseolicola (Pph) 1448A, an economically important pathogen of beans, we observed similar lipid A modifications by mass spectrometric analysis. Therefore, we investigated predicted proteomes of various plant-associated Pseudomonas spp. for putative lipid A-modifying proteins using the well-studied mammalian pathogen Pseudomonas aeruginosa as a reference. We generated isogenic mutant strains of candidate genes and analyzed their lipid A. We show that the function of PagL, LpxO, and EptA is generally conserved in Pph 1448A. PagL-mediated de-acylation occurs at the distal glucosamine, whereas LpxO hydroxylates the secondary acyl chain on the distal glucosamine. The addition of P-EtN catalyzed by EptA occurs at both phosphates of lipid A. Our study characterizes lipid A modifications in vitro and provides a useful set of mutant strains relevant for further functional studies on lipid A modifications in Pph 1448A.
Interferon-β Activity Is Affected by S100B Protein
Authors: Kazakov Alexey S. , Sofin Alexander D. , Avkhacheva Nadezhda V. , Deryusheva Evgenia I. , Rastrygina Victoria A. , Permyakova Maria E. , Uversky Vladimir N. , Permyakov Eugene A. , Permyakov Sergei E.
Published: February 2022

Interferon-β (IFN-β) is a pleiotropic cytokine secreted in response to various pathological conditions and is clinically used for therapy of multiple sclerosis. Its application for treatment of cancer, infections and pulmonary diseases is limited by incomplete understanding of regulatory mechanisms of its functioning. Recently, we reported that IFN-β activity is affected by interactions with S100A1, S100A4, S100A6, and S100P proteins, which are members of the S100 protein family of multifunctional Ca2+-binding proteins possessing cytokine-like activities (Int J Mol Sci. 2020;21(24):9473). Here we show that IFN-β interacts with one more representative of the S100 protein family, the S100B protein, involved in numerous oncological and neurological diseases. The use of chemical crosslinking, intrinsic fluorescence, and surface plasmon resonance spectroscopy revealed IFN-β binding to Ca2+-loaded dimeric and monomeric forms of the S100B protein. Calcium depletion blocks the S100B–IFN-β interaction. S100B monomerization increases its affinity to IFN-β by 2.7 orders of magnitude (equilibrium dissociation constant of the complex reaches 47 pM). Crystal violet assay demonstrated that combined application of IFN-β and S100B (5–25 nM) eliminates their inhibitory effects on MCF-7 cell viability. Bioinformatics analysis showed that the direct modulation of IFN-β activity by the S100B protein described here could be relevant to progression of multiple oncological and neurological diseases.
New Insights on the Nuclear Functions and Targeting of FAK in Cancer
Authors: Pomella Silvia , Cassandri Matteo , Braghini Maria Rita , Marampon Francesco , Alisi Anna , Rota Rossella
Published: February 2022

Focal adhesion kinase (FAK) is a non-receptor tyrosine kinase over-expressed and activated in both adult and pediatric cancers, where it plays important roles in the regulation of pathogenesis and progression of the malignant phenotype. FAK exerts its functions in cancer by two different ways: a kinase activity in the cytoplasm, mainly dependent on the integrin signaling, and a scaffolding activity into the nucleus by networking with different gene expression regulators. For this reason, FAK has to be considered a target with high therapeutic values. Indeed, evidence suggests that FAK targeting could be effective, either alone or in combination, with other already available treatments. Here, we propose an overview of the novel insights about FAK’s structure and nuclear functions, with a special focus on the recent findings concerning the roles of this protein in cancer. Additionally, we provide a recent update on FAK inhibitors that are currently in clinical trials for patients with cancer, and discuss the challenge and future directions of drug-based anti-FAK targeted therapies.