bluuebunny/crossref_metadata_2025 huggingface dataset:
Application of Machine Learning to Predict the Acoustic Cavitation Threshold of Fluids
Authors: Yakupov Bulat , Smirnov Ivan
Published: May 2023

The acoustic cavitation of fluids, as well as related physical and chemical phenomena, causes a variety of effects that are highly important in technological processes and medicine. Therefore, it is important to be able to control the conditions that allow cavitation to begin and progress. However, the accurate prediction of acoustic cavitation is dependent on a complex relationship between external influence parameters and fluid characteristics. The multiparameter problem restricts the development of successful theoretical models. As a result, it is critical to identify the most important parameters influencing the onset of the cavitation process. In this paper, the ultrasonic frequency, hydrostatic pressure, temperature, degassing, density, viscosity, volume, and surface tension of a fluid were investigated using machine learning to determine their significance in predicting acoustic cavitation strength. Three machine learning models based on support vector regression (SVR), ridge regression (RR), and random forest (RF) algorithms with different input parameters were trained. The results showed that the SVM algorithm performed better than the other two algorithms. The parameters affecting the active cavitation nuclei, namely hydrostatic pressure, ultrasound frequency, and outgassing degree, were found to be the most important input parameters influencing the prediction of the cavitation threshold. Other parameters have a minor impact when compared to the first three, and their role can be compensated for by alternative variables. The further development of the obtained results provides a new way to optimize and improve existing theoretical models.
Effect of Preliminary Treatment by Pulsed Electric Fields and Blanching on the Quality of Fried Sweet Potato Chips
Authors: Liu Caiyun , Lv Minming , Du Huihui , Deng Haoyu , Zhou Lu , Li Piaoran , Li Xuxian , Li Baoguo
Published: May 2023

The effects of pulsed electric fields (PEF) and blanching pretreatments on frying kinetics, oil content, color, texture, acrylamide (AA) content, and microstructure have been investigated in this paper. The total PEF pretreatment duration was tPEF = 0.2 s with an intensity of E = 1 kV/cm; blanching was studied at 85 °C for 5 min. The results demonstrated that pretreatment significantly reduced the moisture ratio and oil content by 25% and 40.33%, respectively. The total color change ΔE value of the pretreated samples was lower than that of the untreated samples. In addition, pretreatment increased the hardness of the sample after frying, and the AA content in the fried samples pretreated with PEF + blanching was reduced by approximately 46.10% (638 μg/kg). Finally, fried sweet potato chips obtained by the combined pretreatment exhibited a smoother and flatter cross-sectional microstructure.
Immunomodulatory and Antioxidant Effects of Spray-Dried Encapsulated Kale Sprouts after In Vitro Gastrointestinal Digestion
Authors: Ortega-Hernández Erika , Camero-Maldonado Ana Victoria , Acevedo-Pacheco Laura , Jacobo-Velázquez Daniel A. , Antunes-Ricardo Marilena
Published: May 2023

The health-related compounds present in kale are vulnerable to the digestive process or storage conditions. Encapsulation has become an alternative for their protection and takes advantage of their biological activity. In this study, 7-day-old Red Russian kale sprouts grown in the presence of selenium (Se) and sulfur (S) were spray-dried with maltodextrin to assess their capacity to protect kale sprout phytochemicals from degradation during the digestion process. Analyses were conducted on the encapsulation efficiency, particle morphology, and storage stability. Mouse macrophages (Raw 264.7) and human intestinal cells (Caco-2) were used to assess the effect of the intestinal-digested fraction of the encapsulated kale sprout extracts on the cellular antioxidant capacity, the production of nitric oxide (NOx), and the concentrations of different cytokines as indicators of the immunological response. The highest encapsulation efficiency was observed in capsules with a 50:50 proportion of the hydroalcoholic extract of kale and maltodextrin. Gastrointestinal digestion affected compounds’ content in encapsulated and non-encapsulated kale sprouts. Spray-dried encapsulation reduced the phytochemicals’ degradation during storage, and the kale sprouts germinated with S and Se showed less degradation of lutein (35.6%, 28.2%), glucosinolates (15.4%, 18.9%), and phenolic compounds (20.3%, 25.7%), compared to non-encapsulated ones, respectively. S-encapsulates exerted the highest cellular antioxidant activity (94.2%) and immunomodulatory activity by stimulating IL-10 production (88.9%) and COX-2 (84.1%) and NOx (92.2%) inhibition. Thus, encapsulation is an effective method to improve kale sprout phytochemicals’ stability and bioactivity during storage and metabolism.
Effects of CaCl2 on 3D Printing Quality of Low-Salt Surimi Gel
Authors: Wang Chaoye , Ma Mengjie , Wei Yabo , Zhao Yunfeng , Lei Yongdong , Zhang Jian
Published: May 2023

In order to develop low-salt and healthy surimi products, we limited the amount of NaCl to 0.5 g/100 g in this work and studied the effect of CaCl2 (0, 0.5, 1.0, 1.5, and 2.0 g/100 g) on the 3D printing quality of low-salt surimi gel. The results of rheology and the 3D printing showed that the surimi gel with 1.5 g/100 g of CaCl2 added could squeeze smoothly from the nozzle and had good self-support and stability. The results of the chemical structure, chemical interaction, water distribution, and microstructure showed that adding 1.5 g/100 g of CaCl2 could enhance the water-holding capacity and mechanical strength (the gel strength, hardness, springiness, etc.) by forming an orderly and uniform three-dimensional network structure, which limited the mobility of the water and promoted the formation of hydrogen bonds. In this study, we successfully replaced part of the salt in surimi with CaCl2 and obtained a low-salt 3D product with good printing performance and sensory properties, which could provide theoretical support for the development of healthy and nutritious surimi products.
Composition of Sugars, Organic Acids, Phenolic Compounds, and Volatile Organic Compounds in Lingonberries (Vaccinium vitis-idaea L.) at Five Ripening Stages
Authors: Amundsen Mathias , Hykkerud Anne Linn , Kelanne Niina , Tuominen Sanni , Schmidt Gesine , Laaksonen Oskar , Yang Baoru , Martinussen Inger , Jaakola Laura , Aaby Kjersti
Published: May 2023

Wild lingonberries are a traditional source of food in the Nordic countries and an important contributor to economic activity of non-wood forest products in the region. Lingonberries are a rich source of bioactive compounds and can be a valuable contributor to a healthy diet. However, there are few studies available on how the bioactive compounds in lingonberries develop as they ripen. In this investigation, we examined the content of 27 phenolic compounds, three sugars, four organic acids, and 71 volatile organic compounds at five ripening stages. The study showed that, while the highest content of phenolic compounds was found early in the development, the organoleptic quality of the fruits improved as they ripened. From the first to the last stage of development, anthocyanins went from being nearly absent to 100 mg/100 g fw, and there was an increased content of sugars from 2.7 to 7.2 g/100 g fw, whereas the content of organic acids decreased from 4.9 to 2.7 g/100 g fw, and there were several changes in the profile of volatiles. The contents of flavonols, cinnamic acid derivatives, flavan-3-ols, and the total concentration of phenolic compounds were significantly lower in the fully ripe berries compared to berries in the early green stage. In addition to the changes occurring due to ripening, there was observed variation in the profile of both phenolic compounds and volatiles, depending on the growth location of the berries. The present data are useful for the assessment of harvest time to obtain the desired quality of lingonberries.
Microwave-Induced Rapid Shape Change of 4D Printed Vegetable-Based Food
Authors: Chen Xiaohuan , Zhang Min , Tang Tiantian
Published: May 2023

Microwave heating acts as an environmental stimulus factor to induce rapid shape changes in 4D-printed stereoscopic models over time. The influence of microwave power and model structure on the shape change behavior was explored, and the applicability of the deformed method to other vegetable-based gels was verified. The results described that the G′, G″, η, and proportion of bound water of yam gels increased with the increase in yam powder content, and the yam gel with 40% content had the best printing effect. The IR thermal maps showed the microwaves first gathered in the designed gully region caused the swelling phenomenon, which induced the printed sample to undergo a bird-inspired “spreading of wings” process within 30 s. Increasing the microwave power and microwave heating time were able to increase the bending angles and dehydration rates of the printed samples, thus improving the deformed degree and deformed speed. Different model base thicknesses (4, 6, 8, and 10 mm) also had significant effects on the shape change of the printed structures. The efficiency of the shape changes of 4D-printed structures under microwave induction can be judged by studying the dielectric properties of the materials. In addition, the deformed behaviors of other vegetable gels (pumpkin and spinach) verified the applicability of the 4D deformed method. This study aimed to create 4D-printed food with personalized and rapid shape change behavior, providing a basis for the application scenarios of 4D-printed food.
Edible Insects in Thailand: An Overview of Status, Properties, Processing, and Utilization in the Food Industry
Authors: Krongdang Sasiprapa , Phokasem Patcharin , Venkatachalam Karthikeyan , Charoenphun Narin
Published: May 2023

Edible insects have become increasingly popular in Thailand as a nutritious and appealing alternative food source. As the edible insect industry in the country expands rapidly, efforts are being made to transform it into an economically viable sector with substantial commercial potential. Some of the most consumed and sold edible insects in Thailand include locusts, palm weevils, silkworm pupae, bamboo caterpillars, crickets, red ants, and giant water bugs. With its strong growth, Thailand has the potential to emerge as a global leader in the production and promotion of edible insect products. Edible insects are an excellent source of protein, fat, vitamins, and minerals. In particular, crickets and grasshoppers are protein-rich, with the average protein content of edible insects ranging from 35 to 60 g/100 g of dry weight or 10 to 25 g/100 g of fresh weight. This surpasses the protein content of many plant-based sources. However, the hard exoskeleton of insects, which is high in chitin, can make them difficult to digest. In addition to their nutritional value, edible insects contain biologically active compounds that offer various health benefits. These include antibacterial, anti-inflammatory, anti-collagenase, elastase-inhibitory, α-glucosidase-inhibitory, pancreatic lipase-inhibitory, antidiabetic/insulin-like/insulin-like peptide (ApILP), antidiabetic, anti-aging, and immune-enhancing properties. The Thai food industry can process and utilize edible insects in diverse ways, such as low-temperature processing, including refrigeration and freezing, traditional processing techniques, and incorporating insects into products, such as flour, protein, oil, and canned food. This review offers a comprehensive overview of the status, functional properties, processing, and utilization of edible insects in Thailand, and it serves as a valuable resource for those interested in edible insects and provides guidance for their application in various fields.
Stress Adaptation Responses of a Listeria monocytogenes 1/2a Strain via Proteome Profiling
Authors: D’Onofrio Federica , Schirone Maria , Paparella Antonello , Krasteva Ivanka , Tittarelli Manuela , Pomilio Francesco , Iannetti Luigi , D’Alterio Nicola , Luciani Mirella
Published: May 2023

Listeria monocytogenes is a foodborne pathogen that is ubiquitous and largely distributed in food manufacturing environments. It is responsible for listeriosis, a disease that can lead to significant morbidity and fatality in immunocompromised patients, pregnant women, and newborns. Few reports have been published about proteome adaptation when L. monocytogenes is cultivated in stress conditions. In this study, we applied one-dimensional electrophoresis and 2D-PAGE combined with tandem mass spectrometry to evaluate proteome profiling in the following conditions: mild acid, low temperature, and high NaCl concentration. The total proteome was analyzed, also considering the case of normal growth-supporting conditions. A total of 1,160 proteins were identified and those related to pathogenesis and stress response pathways were analyzed. The proteins involved in the expression of virulent pathways when L. monocytogenes ST7 strain was grown under different stress conditions were described. Certain proteins, particularly those involved in the pathogenesis pathway, such as Listeriolysin regulatory protein and Internalin A, were only found when the strain was grown under specific stress conditions. Studying how L. monocytogenes adapts to stress can help to control its growth in food, reducing the risk for consumers.
The Effect of Guisangyou Tea on Abnormal Lipid Metabolism in Mice Induced by High-Fat Diet
Authors: Zhu Yan , Zhou Xianghui , Ling Nan , Yu Qiming , Wang Huijuan , Du Qizhen
Published: May 2023

This study was aimed to investigate the effect of Guisangyou tea (GSY tea) in improving abnormal lipid metabolism in mice with obesity induced by a high-fat diet (HFD). The results showed that intervention of the water extract of GSY tea (WE) decreased serum levels of lipids, and positively regulated the related antioxidant enzyme activities and the inflammatory factors in the serum and liver. In the liver, the mRNA and protein expression levels of sterol regulatory element-binding proteins-1 (SREBP-1), stearoyl-CoA desaturase-1 (SCD-1), fatty acid synthase (FASN), and acetyl CoA carboxylase (ACC) related to lipid synthesis were downregulated, and the mRNA and protein expression levels of the farnesoid X receptor (FXR) and small heterodimer partner (SHP) related to bile acid production were upregulated. The results demonstrate that GSY tea can improve abnormal lipid metabolism in obese mice by improving the body’s antioxidant capacity, regulating the inflammatory state, and reducing the synthesis of lipids and increasing the production of bile acids. GSY tea can be processed and utilized as a safe and effective resource for improving abnormal lipid metabolism.
Development and Validation of a High-Performance Liquid Chromatography–Tandem Mass Spectrometry Method to Determine Promethazine and Its Metabolites in Edible Tissues of Swine
Authors: Wen Dehui , Shi Rong , He Haiming , Chen Rundong , Zhang Yingzi , Liu Rong , Chen Hong
Published: May 2023

This study aimed to determine promethazine (PMZ) and its metabolites, promethazine sulfoxide (PMZSO) and monodesmethyl-promethazine (Nor1PMZ), in swine muscle, liver, kidney, and fat. A sample preparation method and high-performance liquid chromatography–tandem mass spectrometry (LC–MS/MS) analysis were established and validated. The samples were extracted using 0.1% formic acid–acetonitrile and purified with acetonitrile-saturated n-hexane. After concentration by rotary evaporation, the extract was re-dissolved in a mixture of 0.1% formic acid-water and acetonitrile (80:20, v/v). Analysis was performed using a Waters Symmetry C18 column (100 mm × 2.1 mm i.d., 3.5 μm) with 0.1% formic acid–water and acetonitrile as the mobile phase. The target compounds were determined using positive ion scan and multiple reaction monitoring. PMZ and Nor1PMZ were quantified with deuterated promethazine (PMZ-d6) as the internal standard, while PMZSO was quantified using the external standard method. In spiked muscle, liver, and kidney samples, the limits of detection (LOD) and limits of quantification (LOQ) for PMZ and PMZSO were 0.05 μg/kg and 0.1 μg/kg, respectively, while for Nor1PMZ, these values were 0.1 μg/kg and 0.5 μg/kg, respectively. For spiked fat samples, the LOD and LOQ for all three analytes were found to be 0.05 μg/kg and 0.1 μg/kg, respectively. The sensitivity of this proposed method reaches or exceeds that presented in previous reports. The analytes PMZ and PMZSO exhibited good linearity within the range of 0.1 μg/kg to 50 μg/kg, while Nor1PMZ showed good linearity within the range of 0.5 μg/kg to 50 μg/kg, with correlation coefficients (r) greater than 0.99. The average recoveries of the target analytes in the samples varied from 77% to 111%, with the precision fluctuating between 1.8% and 11%. This study developed, for the first time, an HPLC–MS/MS method for the determination of PMZ, PMZSO, and Nor1PMZ in four swine edible tissues, comprehensively covering the target tissues of monitoring object. The method is applicable for monitoring veterinary drug residues in animal-derived foods, ensuring food safety.
Coupled System of Fractional Impulsive Problem Involving Power-Law Kernel with Piecewise Order
Authors: Ali Arshad , Ansari Khursheed J. , Alrabaiah Hussam , Aloqaily Ahmad , Mlaiki Nabil
Published: May 2023

In this research paper, we study a coupled system of piecewise-order differential equations (DEs) with variable kernel and impulsive conditions. DEs with variable kernel have high flexibility due to the freedom of changing the kernel. We study existence and stability theory and derive sufficient conditions for main results of the proposed problem. We apply Scheafer’s fixed point theorem and Banach fixed point theorem for the result of at least one and unique solution, respectively. In addition, stability results based on the Ulam–Hyers concept are derived. Being a coupled system of piecewise fractional-order DEs with variable kernel and impulsive effects, the obtained results have multi-dimension applications. To demonstrate the applications, we apply the derived results to a numerical problem.
Hybrid Cryogels with Superabsorbent Properties as Promising Materials for Penicillin G Retention
Authors: Dumitru Marinela Victoria , Sandu Teodor , Miron Andreea , Zaharia Anamaria , Radu Ionuț Cristian , Gavrilă Ana-Mihaela , Sârbu Andrei , Iovu Horia , Chiriac Anita-Laura , Iordache Tanța Verona
Published: May 2023

This present study describes the investigation of new promising hybrid cryogels able to retain high amounts of antibiotics, specifically penicillin G, using chitosan or chitosan–biocellulose blends along with a naturally occurring clay, i.e., kaolin. In order to evaluate and optimize the stability of cryogels, three types of chitosan were used in this study, as follows: (i) commercial chitosan; (ii) chitosan prepared in the laboratory from commercial chitin; and (iii) chitosan prepared in the laboratory from shrimp shells. Biocellulose and kaolin, previously functionalized with an organosilane, were also investigated in terms of their potential to improve the stability of cryogels during prolonged submergence under water. The organophilization and incorporation of the clay into the polymer matrix were confirmed by different characterization techniques (such as FTIR, TGA, SEM), while their stability in time underwater was investigated by swelling measurements. As final proof of their superabsorbent behavior, the cryogels were tested for antibiotic adsorption in batch experiments, in which case cryogels based on chitosan extracted from shrimp shells seem to exhibit excellent adsorption properties for penicillin G.
Genome-Wide Identification of the NPR1-like Gene Family in Solanum tuberosum and Functional Characterization of StNPR1 in Resistance to Ralstonia solanacearum
Authors: He Fumeng , Kong Dexing , Feng Zhe , Xu Yongqing , Yuan Qiang , Liu Dan , Wang Xue , Feng Xu , Li Fenglan
Published: May 2023

The NPR1 (nonexpressor of pathogenesis-related genes 1) gene is an activator of the systemic acquisition of resistance (SAR) in plants and is one of the central factors in their response to pathogenic bacterial infestation, playing an important role in plant disease resistance. Potato (Solanum tuberosum) is a crucial non-grain crop that has been extensively studied. However, the identification and analysis of the NPR1-like gene within potato have not been understood well. In this study, a total of six NPR1-like proteins were identified in potato, and phylogenetic analysis showed that the six NPR1-like proteins in Solanum tuberosum could be divided into three major groups with NPR1-related proteins from Arabidopsis thaliana and other plants. Analysis of the exon–intron patterns and protein domains of the six NPR1-like genes from potato showed that the exon–intron patterns and protein domains of the NPR1-like genes belonging to the same Arabidopsis thaliana subfamily were similar. By performing quantitative real-time PCR (qRT-PCR) analysis, we found that six NPR1-like proteins have different expression patterns in different potato tissues. In addition, the expression of three StNPR1 genes was significantly downregulated after being infected by Ralstonia solanacearum (RS), while the difference in the expression of StNPR2/3 was insignificant. We also established potato StNPR1 overexpression lines that showed a significantly increased resistance to R. solanacearum and elevated activities of chitinase, β-1,3-glucanase, and phenylalanine deaminase. Increased peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT) activities, as well as decreased hydrogen peroxide, regulated the dynamic balance of reactive oxygen species (ROS) in the StNPR1 overexpression lines. The transgenic plants activated the expression of the genes associated with the Salicylic acid (SA) defense response but suppressed the expression of the genes associated with Jasmonic acid (JA) signaling. This resulted in resistance to Ralstonia solanacearum.
Benefits of Best Practice Guidelines in Spine Fusion: Comparable Correction in AIS with Higher Density and Fewer Complications
Authors: Fernandes Pedro , Flores Isabel , Soares do Brito Joaquim
Published: May 2023

Background: There is significant variability in surgeons’ instrumentation patterns for adolescent idiopathic scoliosis surgery. Implant density and costs are difficult to correlate with deformity correction, safety, and quality of life measures. Materials and Methods: Two groups of postoperative adolescents were compared based on exposure to a best practice guidelines program (BPGP) introduced to decrease complications. Hybrid and stainless steel constructs were dropped, and posterior-based osteotomies, screws, and implant density were increased to 66.8 ± 12.03 vs. 57.5 ± 16.7% (p < 0.001). The evaluated outcomes were: initial and final correction, rate of correction loss, complications, OR returns, and SRS-22 scores (minimum two-year follow-up). Results: 34 patients were operated on before BPGP and 48 after. The samples were comparable, with the exceptions of a higher density and longer operative times after BPGP. Initial and final corrections before BPGP were 67.9° ± 22.9 and 64.6° ± 23.7; after BPGP, the corrections were 70.6° ± 17.4 and 66.5° ± 14.9 (sd). A regression analysis did not show a relation between the number of implants and postoperative correction (beta = −0.116, p = 0.307), final correction (beta = −0.065, p = 0.578), or loss of correction (beta= −0.137, p = 0.246). Considering screw constructs only (n = 63), a regression model controlled for flexibility continued to show a slight negative effect of density on initial correction (b = −0.274; p = 0.019). Only with major curve concavity was density relevant in initial correction (b = 0.293; p = 0.038), with significance at 95% not being achieved for final correction despite a similar beta (b = 0.263; p = 0.069). Complications and OR returns dropped from 25.6% to 4.2%. Despite this, no difference was found in SRS-22 (4.30 ± 0.432 vs. 4.42 ± 0.39; sd) or subdomain scores pre- and post-program. Findings: Although it appears counterintuitive that higher density, osteotomies, and operative time may lead to fewer complications, the study shows the value of best practice guidelines in spinal fusions. It also shows that a 66% implant density leads to better safety and efficacy, avoiding higher costs.
The Impact of Regional COVID-19 Outbreak on Consumers’ Risk Perception of Purchasing Food Online
Authors: Liu Weijun , Cao Mengzhen , Florkowski Wojciech J.
Published: May 2023

This paper examines the perception of risk associated with the presence of coronavirus in food purchased online and online vs. offline food shopping during the COVID-19 epidemic. The influence of COVID-19 status on risk perception was tested using the data collected from 742 consumers between December 2021 and January 2022. The empirical approach distinguished between the epidemic’s status in a province (or region), city, and other areas of the country and applied the ordered logit technique. The regional and citywide epidemic increased the perception that online purchases carry the virus and are riskier than those made offline. Further examination showed that the regional/provincial epidemic created the perception that packaging or social media use were risk factors when purchasing food online. Heterogeneity analysis showed that risk perception was significantly higher in affected cities than in non-affected provinces or other provinces. Risk perception differed across five online food categories, with the highest levels for online-ordered meals and fresh products. Strengthening COVID-19 prevention and control in cities and the province, managing risk due to the handling of food purchased online, and government monitoring of social media use will lessen consumers’ risk perceptions and encourage the use of online food offers during epidemics.
Feasibility Using Telehealth for Planning Use of Extracorporeal Shockwave Therapy in a Sports Medicine Clinic
Authors: Eckley Marissa J. , Hsu Connie , Tenforde Adam S.
Published: May 2023

(1) Background: The purpose of this study is to describe whether telehealth compared with in-person visits, led to a similar agreement of primary diagnosis reached at the time of procedure using extracorporeal shockwave therapy. (2) Methods: This retrospective study consisted of chart reviews of all new patients evaluated in a sports medicine clinic prior to performing extracorporeal shockwave therapy from April 2020 to March 2021. The primary outcome of the study was describing agreement in primary diagnosis at the time of evaluation (telehealth and in-person) and during the procedure using extracorporeal shockwave therapy. Logistic regression was utilized to identify patient characteristics that may predict agreement of diagnosis using telehealth. (3) Results: The chart review identified 166 patients (45 telehealth and 121 in-person) evaluated for extracorporeal shockwave therapy. Agreement of diagnosis was similar for patients evaluated using telehealth compared to in-person visits (84% vs. 92%, Χ2 = 1.90, p = 0.168). Agreement on diagnosis was more likely in patients who started shockwave within the 1 week of initial visit (OR = 8.27, 95% CI = 1.69–45.29), patients over age 60 (OR = 0.94, 95% CI = 0.90–0.99), and in patients without a history of osteoarthritis (OR = 14.00, 95% CI = 1.88–113.46). (4) Conclusions: Telehealth resulted in a similar agreement to in-person visits to identify a primary diagnosis for planning extracorporeal shockwave therapy. Telehealth may be a reasonable alternative to in-person visits for procedural planning of extracorporeal shockwave therapy.
Ship Archaeology and Material Culture: Artifacts, Representations, Structures, Narratives, and Materialities (16th–19th Centuries)
Authors: Crespo-Solana Ana , Casimiro Tania Manuel
Published: May 2023

This Special Issue is a compilation of studies on underwater and maritime sites related to Early Modern ships and shipwrecks. [...]
Citrus Specialization or Crop Diversification: The Role of Smallholder’s Subjective Risk Aversion and Case Evidence from Guangxi, China
Authors: Chen Xinjian , Xia Mengyao , Zeng Di , Fan Xiaojun
Published: May 2023

Specialization may lead to higher income for small-scale farmers but comes with increased risks, while diversification can mitigate risks and foster agricultural sustainability. Considering the influences of complex risks and farmers’ subjective risk aversion, the decision for small-scale farmers to specialize in citrus cultivation or diversify with multiple crops remains uncertain. There is currently limited understanding of this issue among citrus smallholders in rural China. This study aims to fill this empirical gap by examining the impact of smallholder farmers’ subjective risk aversion on their choice between citrus monoculture and crop diversification. It utilizes a subjective risk assessment approach that incorporates farmers’ risk perceptions and risk attitudes towards citrus farming. Farm crop diversification is assessed through the utilization of both the count index and Shannon index. The empirical analysis employs survey data obtained from citrus growers in Guangxi, China, and applies an instrumental variable regression method with endogeneity consideration using the IV-Probit model and 2SLS model estimation. The results reveal that both risk perceptions and risk attitudes play important roles in citrus smallholders’ land allocation decisions. Specifically, citrus farmers who perceive higher risks and adopt risk-averse attitudes are statistically more inclined to engage in land use diversification practices, including the practice of growing citrus as well as other crops, which contributes to reducing the risks of citrus farming and promoting local environmental conservation. These results contribute to a better scholarly comprehension of the relationship between risk perceptions, risk attitudes, and crop diversification among small-scale citrus farmers. They provide valuable insights for enhancing the sustainability of land use systems with citrus farming while also emphasizing the importance of maintaining essential diversification in small-scale farming throughout the process of agricultural modernization.
Zinc Oxide Nanoparticles Enhanced Growth of Tea Trees via Modulating Antioxidant Activity and Secondary Metabolites
Authors: Chen Chen , Lai Jiaying , Chen Hong , Yu Fangyuan
Published: May 2023

Nano-fertilizer has been dubbed ‘the fertilizer of the 21st century’, and it is already being used extensively in agriculture. Zinc oxide nanoparticles (ZnO-NPs) have excellent biological properties and are expected to be an ideal choice for plant zinc fertilizer. Tea is one of the top three beverages in the world, and improving the quality of tea is a priority in its research field. In this study, different concentrations (0, 10, 50, 100, 150, and 200 mg·L−1) of ZnO-NPs were sprayed on tea leaves to investigate their effects on volatile aroma substances and biochemical aspects of tea leaves. The results revealed that various concentrations of ZnO-NPs had different effects on physiological indexes. The concentration of 150 mg·L−1 of ZnO-NPs enhanced chlorophyll content, while the 100 mg·L−1 concentration of ZnO-NPs promoted the accumulation of soluble proteins and the activity of antioxidant enzymes, including a decrease in the content of malondialdehyde. In addition, the ZnO-NPs spray reduced the content of tea polyphenols. A total of 27 volatiles were identified under six treatments, with benzene being the common compound with an average content of 45.97%. Ethanolamine and cis-3-hexenyl acetate were the other two major compounds. It was concluded that the presence of ZnO-NPs improved the antioxidant system of teas, increased soluble protein content and provided better reactive oxygen species protection for plants, especially in the case of ZnO-NPs at 100 mg·L−1. We highlighted that ZnO-NPs application was a favorable way to improve tea trees growth.
Symbiosis of Arbuscular Mycorrhizal Fungi and Lycium barbarum L. Prefers NO3− over NH4+
Authors: Gong Minggui , Zhang Qiaoming , Cheng Kang , Zhang Haoqiang
Published: May 2023

Nitrogen (N) is an essential nutrient that plants require and is, most of the time, limited in different terrestrial ecosystems. Forming symbioses with plants, arbuscular mycorrhizal (AM) fungi improve mineral element uptake and the net primary production of plants. Recent reports have suggested that AM fungi mediate N uptake in plants. However, there are fewer studies on the influence of AM fungi on the response of Lycium barbarum, a medicinal plant in northwest China, under different N-addition conditions. In this study, the effect of Rhizophagus irregularis, N forms (NO3− and NH4+), and N levels (1.5, 7.5, 15, 30 mM) on the performance of L. barbarum was evaluated through a pot experiment. The application of R. irregularis significantly improved L. barbarum biomass, net photosynthetic rate, and root tissue viability under adequate NO3− and NH4+ supplies, and mycorrhizal plants showed better performance under NO3− supply. AM colonization enhanced N acquisition under adequate NO3− supply and strongly induced the expression of LbAMT3-1 in L. barbarum roots. Based on these results, we propose that NO3−-dominated N supply favors mycorrhizal symbiosis to a greater extent than NH4+; this study provides a basis for maintaining beneficial AM symbiosis during nitrogen fertilizer use in arable land.
‘The Addiction Was Making Things Harder for My Mental Health’: A Qualitative Exploration of the Views of Adults and Adolescents Accessing a Substance Misuse Treatment Service
Authors: Spencer Liam , Alderson Hayley , Scott Steph , Kaner Eileen , Ling Jonathan
Published: May 2023

The relationship between substance use and mental health is complex, and both constitute a global public health burden. In the UK, the estimated annual financial costs of alcohol-related harm and illicit drug use are GBP 21.5 billion and GBP 10.7 billion, respectively. This issue is magnified in the North East of England, where treatment access is low and a large proportion of individuals experience socioeconomic deprivation. The present study aimed to explore the experiences of adults and adolescents accessing a substance misuse treatment service in the North East, in order to inform policy makers, commissioners, and providers of substance misuse treatment and prevention. Semi-structured qualitative interviews were conducted with an opportunistic sample of n = 15 adult participants (aged 18 years and over) and n = 10 adolescent participants (aged between 13 and 17 years). Interviews were audio-recorded, transcribed, anonymised, and analysed thematically. Five key themes were identified: (1) initiation of substance use, (2) early life experiences, (3) the bi-directional relationship of mental health and substance use, (4) cessation of substance use, and (5) accessing treatment. Future preventative interventions should focus on providing support to individuals who have been exposed to adverse childhood experiences, with treatment provision for individuals experiencing co-occurring mental health and substance use issues taking a more holistic approach.
Job Seekers’ Burnout and Engagement: A Qualitative Study of Long-Term Unemployment in Italy
Authors: Nonnis Marcello , Agus Mirian , Frau Gianmarco , Urban Antonio , Cortese Claudio Giovanni
Published: May 2023

Long-term unemployment has major consequences from an economic, physical and psychosocial perspective. Several authors have pointed out that the search for employment is in itself work, which can generate feelings of exhaustion of psychophysical energies, cynicism and disinvestment, as well as a sense of ineffectiveness to the point of complete disillusion. The construct of burnout can be used to describe this psychological process. This study evaluated the burnout and engagement dimensions in individuals searching for work for a long time, from a qualitative perspective. Fifty-six semi-structured interviews were conducted with a sample of long-term unemployed job seekers (Sardinia, Italy), based on Maslach’s model of burnout (exhaustion, cynicism, effectiveness in job search). The answers to the semi-structured interviews were processed through T-Lab, a semi-automatic textual analysis software. Four thematic cores emerged: exhaustion vs. engagement, cynicism vs. trust, inefficacy vs. efficacy in job search and disillusion vs. hope. This result is consistent with the four-dimensional theoretical model of burnout, originally proposed by Edelwich and Brodsky, recently taken up by Santinello, and framed as the opposite of engagement, as shown in the JD-R model. This study highlights that burnout can describe the psychosocial experiences of long-term unemployed job seekers.
A Theoretically Informed Critical Review of Research Applying the Concept of Liminality to Understand Experiences with Cancer: Implications for a New Oncological Agenda in Health Psychology
Authors: Stenner Paul , De Luca Picione Raffaele
Published: May 2023

Liminality was described more than 20 years ago as a major category explaining how cancer is experienced. Since then, it has been widely used in the field of oncology research, particularly by those using qualitative methods to study patient experience. This body of work has great potential to illuminate the subjective dimensions of life and death with cancer. However, the review also reveals a tendency for sporadic and opportunistic applications of the concept of liminality. Rather than being developed in a systematic way, liminality theory is being recurrently ‘re-discovered’ in relatively isolated studies, mostly within the realm of qualitative studies of ‘patient experience’. This limits the capacity of this approach to influence oncological theory and practice. In providing a theoretically informed critical review of liminality literature in the field of oncology, this paper proposes ways of systematizing liminality research in line with a processual ontology. In so doing, it argues for a closer engagement with the source theory and data, and with more recent liminality theory, and it sketches the broad epistemological consequences and applications.
Achilles Tendon Repair after Tenorraphy Imaging and the Doughnut Metaphor
Authors: Bisciotti Gian Nicola , Bisciotti Andrea , Auci Alessio , Bisciotti Alessandro , Eirale Cristiano , Corsini Alessandro , Volpi Piero
Published: May 2023

After Achilles tendon tenorraphy, tendon tissue undergoes a long period of biological healing. During this period, tissue turnover shows heterogeneity between its peripheral and central regions. This case report concerns the description of the tendon healing process of an athlete who underwent an Achilles tendon tenorraphy. As the reparative processes progressed, magnetic resonance imaging (MRI) showed centralization of the hyperintensity area and the tendon assumed a doughnut-like appearance. At the same time, ultrasound (US) assessment showed a progressive reorganization of the tendon fibrillar structure. Therefore, for the athlete, MRI and US assessment together represent a useful tool for the decision-making process after Achilles tendon tenorraphy.
New Caregiver Diagnoses of Severe Depression and Child Asthma Controller Medication Adherence
Authors: Currie Janet M. , Mercer Michele , Michael Russ , Pichardo Daniel
Published: May 2023

Background and Objectives: Children with asthma who have depressed caregivers are known to be less adherent to medication regimes. However, it is less clear how adherence responds to a caregiver’s new diagnosis of severe depression or whether there is a similar relationship with other serious caregiver diagnoses. The hypothesis is that adherence worsens both with new diagnoses of depression and possibly with new diagnoses of other serious conditions. Methods: This study follows a cohort of 341,444 continuously insured children with asthma before and after a caregiver’s new diagnosis of severe depression or another serious health condition. The effect of a new depression diagnosis on a child’s medication adherence is compared to the effect of new diagnoses of other common caregiver chronic conditions including diabetes, cancer, congestive heart failure, coronary artery disease, and chronic obstructive pulmonary disease. Results: Results show that children’s medication adherence declines following a caregiver’s new diagnosis of severe depression, but that it also declines following a caregiver’s new diagnosis of diabetes. There is no association with new diagnoses of the other caregiver chronic conditions examined. Conclusions: Children whose caregivers have a new diagnosis of depression or diabetes may be at increased risk of deterioration in their medication adherence. These caregivers may benefit from additional support and follow-up. The relationship between caregivers’ health and children’s medication adherence is complex and deserves further study.
From Social Rejection to Welfare Oblivion: Health and Mental Health in Juvenile Justice in Brazil, Colombia and Spain
Authors: Carbonell Ángela , Georgieva Sylvia , Navarro-Pérez José-Javier , Botija Mercedes
Published: May 2023

(1) Background: This study aims to examine and describe the policies of three Latin American countries: Colombia, Brazil, and Spain, and identify how they implement their support systems for health, mental health, mental health for children and adolescents, and juvenile justice systems that support judicial measures with treatment and/or therapeutic approaches specialized in mental health. (2) Methods: Google Scholar, Medline, and Scopus databases were searched to identify and synthesize of the literature. (3) Results: Three shared categories were extracted to construct the defining features of public policies on mental health care in juvenile justice: (i.) models of health and mental health care, (ii.) community-based child and adolescent mental health care, and (iii.) mental health care and treatment in juvenile justice. (4) Conclusions: Juvenile justice in these three countries lacks a specialized system to deal with this problem, nor have procedures been designed to specifically address these situations within the framework of children’s rights.
Revealing the Influence of the Fine-Scale Built Environment on Urban Rail Ridership with a Semiparametric GWPR Model
Authors: Wang Jianpo , Zhao Meng , Ai Teng , Wang Qushun , Liu Yufan
Published: May 2023

There is a causal interaction between urban rail passenger flow and the station-built environment. Analyzing the implicit relationship can help clarify rail transit operations or improve the land use planning of the station. However, to characterize the built environment around the station area, existing literature generally adopts classification factors in broad categories with strong subjectivity, and the research results are often shown to have case-specific applicability. Taking 154 stations on 8 rail transit lines in Xi’an, China, as an example, this paper uses the data sources of multiple open platforms, such as web map spatial data, mobile phone data, and price data on house purchasing and renting, then combines urban land classification in the China Urban Land Classification and Planning and Construction La1d Standard to classify the land use in the station area using structural hierarchy. On the basis of extracting fine-grained factors of the built environment, a semi-parametric Geographically Weighted Poisson Regression (sGWPR) model is used to analyze the correlation and influence between the variation of passenger flow and environmental factors. The results show that the area of Class II residential land (called R2) is the basis for generating passenger flow demand during morning and evening peak periods; The connection intensity between rail transit station area and bus services has a significant impact on commuters’ utilization level of urban rail transit. Furthermore, two scenarios in practical applications will be provided as guidance according to the research results. This study provides a general analytical framework using urban multi-source data to study the internal relationship and impact between the built environment of urban rail transit stations and passenger flow demand.
Cellulose Acetate-Supported Copper as an Efficient Sustainable Heterogenous Catalyst for Azide-Alkyne Cycloaddition Click Reactions in Water
Authors: Stiriba Salah-Eddine , Bahsis Lahoucine , Benhadria Elhouceine , Oudghiri Khaoula , Taourirte Moha , Julve Miguel
Published: May 2023

A new sustainable heterogeneous catalyst for copper-catalyzed azide-alkyne cycloaddition reaction (CuAAC) was investigated. The preparation of the sustainable catalyst was carried out through the complexation reaction between the polysaccharide cellulose acetate backbone (CA) and copper(II) ions. The resulting complex [Cu(II)-CA] was fully characterized by using different spectroscopic methods such as Fourier-transform infrared spectroscopy (FTIR), Scanning Electron Microscopy (SEM), Energy Dispersive X-ray (EDX), Ultraviolet-visible (UV-vis), and Inductively Coupled Plasma (ICP) analyses. The Cu(II)-CA complex exhibits high activity in the CuAAC reaction for substituted alkynes and organic azides, leading to a selective synthesis of the corresponding 1,4-isomer 1,2,3-triazoles in water as a solvent and working at room temperature. It is worth noting that this catalyst has several advantages from the sustainable chemistry point of view including no use of additives, biopolymer support, reactions carried out in water at room temperature, and easy recovery of the catalyst. These characteristics make it a potential candidate not only for the CuAAC reaction but also for other catalytic organic reactions.
Molecular Signatures in Swine Innate and Adaptive Immune Responses to African Swine Fever Virus Antigens p30/p54/CD2v Expressed Using a Highly Efficient Semliki Forest Virus Replicon System
Authors: Huang Mei , Zheng Hanghui , Tan Weixiong , Xiang Chengwei , Fang Niran , Xie Wenting , Wen Lianghai , Liu Dingxiang , Chen Ruiai
Published: May 2023

African swine fever virus (ASFV) causes a devastating viral hemorrhagic disease in domestic pigs and Eurasian wild boars, posing a foremost threat to the swine industry and pig farming. The development of an effective vaccine is urgently needed, but has been hampered by the lack of an in-depth, mechanistic understanding of the host immune response to ASFV infection and the induction of protective immunity. In this study, we report that immunization of pigs with Semliki Forest Virus (SFV) replicon-based vaccine candidates expressing ASFV p30, p54, and CD2v, as well as their ubiquitin-fused derivatives, elicits T cell differentiation and expansion, promoting specific T cell and humoral immunity. Due to significant variations in the individual non-inbred pigs in response to the vaccination, a personalized analysis was conducted. Using integrated analysis of differentially expressed genes (DEGs), Venn, KEGG and WGCNA, Toll-like receptor, C-type lectin receptor, IL17 receptor, NOD-like receptor and nucleic acid sensor-mediated signaling pathways were demonstrated to be positively correlated to the antigen-stimulated antibody production and inversely correlated to the IFN-γ secreting cell counts in peripheral blood mononuclear cells (PBMCs). An up-regulation of CIQA, CIQB, CIQC, C4BPA, SOSC3, S100A8 and S100A9, and down-regulation of CTLA4, CXCL2, CXCL8, FOS, RGS1, EGR1 and SNAI1 are general in the innate immune response post-the second boost. This study reveals that pattern recognition receptors TLR4, DHX58/DDX58 and ZBP1, and chemokines CXCL2, CXCL8 and CXCL10 may play important roles in regulating this vaccination-stimulated adaptive immune response.