bluuebunny/crossref_metadata_2025 huggingface dataset:
Terra Urbana aforada: estrutura, forma e transformação no espaço urbano original de fundação da cidade de Ribeirão Preto/SP
Authors: Piccinato Junior Dirceu , Salgado Ivone
Published: April 2019

O entendimento da formação do espaço urbano no Brasil não pode se restringir a uma única perspectiva de análise. O objetivo deste artigo é discutir os aspectos morfológicos urbanos das cidades regidas pelo regime jurídico da enfiteuse, situação recorrente no nordeste do estado de São Paulo - Brasil, com especial atenção para a cidade de Ribeirão Preto. Não se pode definir ao certo em que momento o processo de formação do espaço urbano brasileiro ganhou contornos processuais quanto à fundação de uma localidade, entretanto, pode-se reconhecer e dar sentido a um processo que, se não foi o único, foi o mais difundido no estado de São Paulo: o de formação do patrimônio religioso. A concessão de terras à Igreja Católica foi recorrente no Brasil durante os períodos colonial e imperial para a fundação das cidades. Como estas terras eram destinadas à formação de bens patrimoniais de uma devoção santa dos moradores, não podiam ser comercializadas. Aforamento, enfiteuse ou emprazamento são sinônimos de um procedimento jurídico que, ao longo do tempo, mostrou-se bastante flexível em relação ao momento histórico, aos interesses dos diversos agentes sociais e à sua adequação ao meio social. Esse regime jurídico revela uma relação em que há um proprietário – proprietário direto – que tem o controle direto da terra urbana, concedendo a outro, o arrendatário, o domínio útil da terra. A este último lhe cabia o direito de usar a terra e a obrigação de um pagamento anual de impostos e um compromisso de dar uma porcentagem da comercialização da propriedade. A enfiteuse era uma forma de desmembramento da propriedade mediante um contrato. No ano de 1845 alguns fazendeiros doaram um terreno para a formação dos bens fundiários do santo São Sebastião, onde hoje é a cidade de Ribeirão Preto. Essa conjuntura definiu a estrutura, a forma e as transformações do espaço urbano original da cidade.
Construindo uma Ruptura: Tectônica Moderna em Erich Mendelsohn
Authors: Costa Phillipe Cunha , Bueno Guilherme Da Silva
Published: April 2019

O presente artigo visa colaborar no aprofundamento do momento de inflexão na tectônica da arquitetura do início do séc. XX, destacando principalmente o expoente de Erich Mendelsohn, arquiteto alemão radicado nos Estados Unidos a partir dos anos 40. De origem judaica e sofrendo com o retalhamento nazista com a ascensão de Adolf Hitler no poder da Alemanha, Mendelsohn inicia uma produção genuinamente autora que passa a dialogar com produções marginais da arquitetura moderna dominante de seu período, principalmente sob o óculo de Mies Van Der Rohe. Partindo do conceito de que a produção moderna da arquitetura se constitui enquanto uma crescente cultura de expressão tectônica, exemplificada na criação da Bauhaus, podemos entender Mendelsohn como um expoente singular que viria a desempenhar uma linguagem mais radicalmente moderna e, sobretudo, fora de um contexto visivelmente estilístico. Para tal, rastrearemos o ponto de fragmentação em suas obras construídas, afim de entender suas intenções poéticas na construção. Sendo assim, encontra-se destacado sua produção no período de ascensão nazista e posterior instalação no continente norte-americano, entre os anos de 1921, com o final da construção da Torre Einstein, até 1950, com o término da The Park Synagogue em Cleveland Heighs.
Diabetes Mortality and Morbidity Trends and Related Risk Factors in Iranian Adults: An Appraisal via Current Data
Authors: Veisani Yousef , Khazaei Salman , Jenabi Ensiyeh , Delpisheh Ali
Published: April 2019

The abstract not available.
PRODUCT DEVELOPMENT SEASONING OF MADURA SATAY
Authors: Prasnowo M. Adhi , Hidayat Khoirul
Published: April 2019

Purpose of Study: Madura is one area in East Java province which has a wide range of local advantages such as Madura satay, Madura herbal, salt, corn, handicraft sickles, and others. Madura Satay is one of the advantages of a locally owned Madura island which is well known by the people of Indonesia and even to foreign countries. The seasoning of Madura satay is manufactured directly by the original Maduran people, so that consumers difficult to find its specific spices in the market. Therefore, there is a need for research on the development of the seasoning products of Madura satay instant.

Methodology: The method used in this research is the method of Quality Function Deployment.

Result: Based on the results of data analysis and processing, it can be concluded that there are eight consumer needs instant seasoning products of Madura satay, namely; product texture should be soft, there are a rancid taste, taste sweet spicy seasoning, spice brown-golden color, weight 500 grams of product, packaging using clear plastic material, the packaging must be attractive, low prices.

Implications/Applications: The level of customer satisfaction, there are two attributes of customer satisfaction that still need to be improved, namely the product weight of 500 grams and packaging using clear plastic material.
Disruptive Change in Today’s Innovation: A Case Study Analysis of OptimalPlus
Authors: Green Daryl D. , Murimi Renita , Mathson Taylor , Roach Christina , Stafford Rachel ,   Paul Dickinson College of Business, Oklahoma Baptist University, 500 W., University Drive, Shawnee, OK 74804, USA.,   Paul Dickinson College of Business, Oklahoma Baptist University, 500 W., University Drive, Shawnee, OK 74804, USA.,   Paul Dickinson College of Business, Oklahoma Baptist University, 500 W., University Drive, Shawnee, OK 74804, USA.,   Paul Dickinson College of Business, Oklahoma Baptist University, 500 W., University Drive, Shawnee, OK 74804, USA.,   Paul Dickinson College of Business, Oklahoma Baptist University, 500 W., University Drive, Shawnee, OK 74804, USA.
Published: April 2019

In today’s hypercompetitive world, electronics manufacturing companies experience challenges to respond to increasing consumer electronics demand while combatting disruptive change in their industries. The revolution in manufacturing via Industry 4.0 has transformed the way that companies approach supply chain process to gain a stronger understanding of data efficiencies. This case study examines OptimalPlus to demonstrate how its services while tapping into today’s technologies keep pace with competitors during disruptive change in the consumer electronics industry. OptimalPlus is a leader in providing the software solutions necessary for electronics manufacturers to get the most out of its supply chain process. This study explores the concept of Industry 4.0 and the benefits that manufacturers can gain from utilizing technologies. By understanding the benefits of using Industry 4.0, scholars and practitioners can better understand the way to innovate under the lens of disruptive change in the marketplace.
Variation in lactoferrin gene affects milk lactoferrin content and somatic cell count in murrah buffaloes
Authors: Singh Arun Pratap , Ramesha K. P. , Mir M. A. , Arya Ashwani , Isloor S.
Published: April 2019

Lactoferrin plays an important role in antimicrobial defence and is a potential candidate gene for mastitis resistance. In the present investigation, polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) studies were
carried out in Murrah (Bubalus bubalis) buffaloes to detect single nucleotide polymorphisms (SNPs) of lactoferrin gene and to analyse the association between the observed polymorphisms with milk lactoferrin content and Somatic cell count (SCC).
PCR-SSCP analysis revealed a total of 07 different variants in the partial coding region of the lactoferrin gene. PCR-SSCP analysis of exon 10 of lactoferrin gene revealed three and that of exons 4 and 5 revealed two unique patterns each, while all
other exons exhibited monomorphic pattern. Comparison of nucleotide sequences of lactoferrin gene of the Murrah buffaloes with taurine reference sequence revealed a total of 14 point mutations, 09 of which were found to be in coding region.
Conceptualized translation of nucleotide sequence revealed 07 amino acid changes. SSCP variants of exon 10 (P less than 0.01) had significant effect on milk SCC. The SSCP variants of exon 4 and exon 5 had significant (P less than 0.05) effect on
lactoferrin content. The SCC and lactoferrin content in Murrah buffaloes was highest in 4th and above parity group. Stage of lactation had highly significant (P less than 0.01) effect on both milk SCC and lactoferrin content. There was a high and
significant (p less than 0.01) correlation (0.741) between SCC and lactoferrin content in milk. The observed association between SSCP variants in lactoferrin gene with milk SCC and milk lactoferrin content can be used as prognostic markers for
selection of animals for high lactoferrin content and low somatic cell count, as well as a marker of susceptibility/resistance to mastitis in Murrah buffaloes
Reconsidering the motivation of learners in educational computer game contexts
Authors: TÜZÜN Hakan , BARAB Sasha A. , THOMAS Michael K.
Published: April 2019

The purpose of this study was to identify motivational elements of an online multi-player educational computer game that uses a 3D multi-user environment to immerse children ages 9-12 in educational tasks. The methodological approach design ethnography, a process that involves using ethnographic methods when the researchers are both observers and designers of the context being studied, was employed for researching this game. Interviews were completed with twenty participants playing the educational game and prolonged observations were conducted where participants played the game in a natural setting. The constant comparison method of grounded theory was used for analyzing interview and observation records. The qualitative methods used in this study allowed for additional insights into previous research on motivation. In addition to the 5 constructs identified in previous motivation research on videogames (challenge, curiosity, control, fantasy, and choice), 10 additional motivational elements to play the game emerged from an analysis of interviews with and observations of the 20 children participating in this study. These were identity presentation, social relations, playing, learning, achievement, rewards, immersive context, uniqueness, creativity, and context of support.
Explorando o potencial científico-social da coleção científica de fósseis do IGc/USP: preparação de material para pesquisa, exposição e uso didático
Authors: Voltani Cibele Gasparelo , Silva Kelly B. V. , Silva Dayane G. , Baptista Regina H. R. C. , Lookin Debora L. G. , Fairchild Thomas R. , Leme Juliana M.
Published: April 2019

O Laboratório de Paleontologia Sistemática (LPS) do Instituto de Geociências (IGc) da Universidade de São Paulo (USP), possui uma das mais importantes coleções paleontológicas do Brasil. Em 2012, os espécimes provenientes de ações de proteção ao patrimônio fossilifero passaram permanentemente para acervo do IGc, possibilitando sua utilização pelos pesquisadores e pelo público em geral. A maior quantidade deste material é composta por fósseis do eoretáceo da Bacia do Araripe em apreciáveis condições de preservação. Todavia, numerosos espécimes foram adulterados para suprir uma demanda do comércio ilegal. Estas adulterações comprometeram o uso desse material em pesquisas científicas, porém não comprometeram seu uso para a educação e divulgação científica. Os docentes responsáveis pelo LPS optaram pela utilização deste material para abordar assuntos científicos (p. ex., grandes eventos de extinção e tipos de fossilização) e sociais (p. ex., contrabando de fósseis e patrimônio científico). Temas relacionados à paleontologia, ao desenvolvimento do sistema terrestre e a história da vida no planeta são passíveis de contribuição no desenvolvimento de uma visão global do indivíduo como cidadão crítico. Por meio de ações aqui apresentadas é apontada a relevância da formação dos estagiários e a proximidade dos professores da rede de ensino com as pesquisas da universidade.
STUDI PEMANFAATAN KULIT KOPI ARABIKA (Coffee arabica L.) SEBAGAI MIKRO ORGANISME LOKAL (MOL)
Authors: Juwita A Ita , Mustafa Arnida , Tamrin Risna
Published: April 2019

MOL (Mikro Organisme Lokal) is the result of local material decomposition with
fermentation method. MOL contains macro and micro nutrients and microbial decomposers.
The smaller coffee peel that will fermented, the faster MOL can be formed. To accelerate
decomposition in MOL solution, it can be added food source of bacteria such as coconut water
and brown sugar.
The purpose of this study was to study the process of making MOL from coffee peel, to
analyze the content of nitrogen, phosphor, and potassium of MOL of coffee peel and to
determine the effect of fermentation time on nitrogen, phosphor, and potassium contents of
MOL that resulted.
This study was conducted with 4 (four) treatments, week 1, 2, 3 and 4 and 1 factor and
using 2 times replicate. The parameter of testing used for making coffee peel as local
microorganism are MOL volume, nitrogen, phosphor and potassium contents.
The result of study showed that the highest mol volume obtained in week 4 of 8.5 ml and
the lowest in week 1 of 5 ml. They were obtained from 500 g coffee peel. Determination of
nitogen, phosphorus and potassium as a macro nutrients has been conducted. The result showed
that the highest nitrogen was found to be 0,0039% in week 1, 3 and 4 and the lowest in week 2
with percentage 0.034 %. Determine of phosphorus showed that the highest phosphor was
obtained in week 2 wtih percentage 0.033 % and the lowest in week 4 of 0.018%, and the result
of potassium analyze, the highest obtained in week 2 of 0.035 % and the lowest in week 4 of
0.014%
PENENTUAN AKTIVITAS ENZIM MANANASE DARI BERBAGAI MIKROORGANISME DI INDONESIA DAN PERANANNYA DALAM BIDANG PANGAN: KAJIAN PUSTAKA
Authors: Seftiono Hermawan
Published: April 2019

Mananase is an enzyme that is involved in producing prebiotic, namely manooligosaccharide.
In Indonesia, research about mananase enzyme has started by using several types of
microogranisms. Based on available reports, it seems that data collection is needed in order to
determine the most optimal mananase enzyme producer. From among 20 microorganisms, it
showed that Isolat SM – 14 (Bacillus sp.) has 119.44 U / ml activity with 19.55 U / mg specific
activity in locus bean gum substrate. The highest enzyme activity is the most potential when
applied as manooligosaccharide producer in food industry. Manooligosaccharide can be
applied to various products such as cookies, chocolate, candy, and soft drinks.
KARAKTERISTIK MIE KERING DENGAN SUBSTITUSI TEPUNG GEMBILI DAN PENAMBAHAN PLASTIZISER GMS (GLISEROL MONO STEARAT)
Authors: Winarti Sri , Susiloningsih Enny Karti Basuki , Fasroh Ferry Yusuf Zaenal
Published: April 2019

Mie saat ini merupakan salah satu makanan yang sangat digemari semua masyarakat, hal ini didukung dengan kecenderungan dan pola hidup masyarakat modern yang lebih menginginkan makanan siap saji. Kebutuhan terigu yang merupakan bahan dasar pembuatan mie perlu diimbangi dengan upaya substitusi dengan bahan baku local, salah satu alternatif menggunakan tepung umbi gembili (Dioscorea esculenta L.). Meskipun telah dapat dikembangkan menjadi tepung, kelemahan dari sifat fungsional tepung umbi gembili ialah tidak adanya kandungan gluten pada tepung gembili yang mengakibatkan adonan mie kurang elastis. Salah satu upaya untuk memperbaiki kelemahan tersebut adalah dengan penambahan plastisizer yaitu gliserol monostearat untuk memperbaiki karakteristik mie kering. Penelitian ini bertujuan mempelajari pengaruh substitusi tepung gembili dan penambahan gliserol monostearat terhadap kualitas fisikokimia dan organoleptik mie, sehingga dihasilkan mie dengan kualitas baik dan disukai konsumen.
Rancangan penelitian yang digunakan adalah Rancangan Acak Lengkap (RAL) yang terdiri dari 12 perlakuan dan tiga kali ulangan dengan perlakuan substitusi tepung gembili (0%; 15%; 30%; dan 45%) dan penambahan GMS (1%; 2%; 3%). Data dianalisa menggunakan metode two-way ANOVA pada tingkat kepercayaan 95%, Jika ada perbedaan antar perlakuan dilanjutkan dengan Uji Duncan α = 5%,  dan untuk analisa uji organoleptik menggunakan metode hedonik scale scoring dengan perhitungan data menggunakan metode friedman. Hasil penelitian menunjukan mie kering tepung gembili dengan perlakuan substitusi tepung gembili 15% dan penambahan gliserol monostearat 3% merupakan perlakuan terbaik dengan nilai kadar air 8,84%, kadar abu 1,65%, kadar protein 7,50%, kadar pati 55,99%, daya rehidrasi 41,93%, elastisitas 25,69%, cooking loss 7,94%, serta jumlah ranking warna 132,00, aroma 114,50, dan tekstur 183,00
PENGGUNAAN SAW UNTUK ANALISIS PROSES PEREBUSAN KEDELAI DALAM PRODUKSI TEMPE
Authors: Utama Dana Marsetiya , Baroto Teguh
Published: April 2019

This article describes assessment organoleptic quality in boiling process soybean at production tempe. we proposed the model for selecting boiling process soybean based on quality organoleptic. We used pairwise comparison and SAW the problem. Pairwise comparison used to determine to weigh for organoleptic criteria. SAW used for selecting boiling process alternative. the result of proposed pairwise comparison and SAW model showed this model effective for assessment organoleptic quality in boiling process soybean at production tempe.
A large number of cerebral microbleeds in CADASIL patient presented with recurrent seizures: A case report
Authors: Anamnart Chumpol , Songsaeng Dittapong , Chanprasert Sirisak
Published: April 2019

Background

Cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) is a hereditary arteriopathy associated with NOTCH3 gene. Clinical manifestations include strokes, transient ischemic events, psychiatric disturbances, dementia, as well as migraines. We report a case of a Thai man with severe phenotype of CADASIL presenting with recurrent seizures and acute ischemic stroke in whom classic vascular risk factors are also present.

Case presentation

A 50 year-old-man who has had history of mood disorder and progressive cognitive decline for 20 years as well as well-controlled diabetes mellitus and hypertension presenting with recurrent generalized seizures and acute right-sided weakness. The MRI of the brain showed acute infarction of the left pons, a large number of cerebral microbleeds throughout the brain and white matter abnormalities without classic anterior temporal lobe lesion. Molecular genetic testing identified homozygous pathologic variants, c.1672C>T (p. Arg558Cys) in the NOTCH3 gene. The diagnosis of CADASIL was confirmed. His clinical symptoms deteriorated, and he died of fever of unknown origin with secretion obstruction.

Conclusion

This case raises the awareness of an uncommon cause of acute ischemic stroke in patients with classic vascular risk factors and highlights the complete evaluation for an unexpected clinical presentation or unexpected diagnostic study results.
Metabolic Reprogramming of Cancer by Chemicals that Target Glutaminase Isoenzymes
Authors: Matés José M. , Campos-Sandoval José A. , de los Santos-Jiménez Juan , Segura Juan A. , Alonso Francisco J. , Márquez Javier
Published: April 2019

Background:

Metabolic reprogramming of tumours is a hallmark of cancer. Among the
changes in the metabolic network of cancer cells, glutaminolysis is a key reaction altered in neoplasms.
Glutaminase proteins control the first step in glutamine metabolism and their expression
correlates with malignancy and growth rate of a great variety of cancers. The two types of glutaminase
isoenzymes, GLS and GLS2, differ in their expression patterns and functional roles: GLS
has oncogenic properties and GLS2 has been described as a tumour suppressor factor.

Results:

We have focused on glutaminase connections with key oncogenes and tumour suppressor
genes. Targeting glutaminase isoenzymes includes different strategies aimed at deactivating the
rewiring of cancer metabolism. In addition, we found a long list of metabolic enzymes, transcription
factors and signalling pathways dealing with glutaminase. On the other hand, a number of chemicals
have been described as isoenzyme-specific inhibitors of GLS and/or GLS2 isoforms. These molecules
are being characterized as synergic and therapeutic agents in many types of tumours.

Conclusion:

This review states the metabolic pathways that are rewired in cancer, the roles of glutaminase
isoforms in cancer, as well as the metabolic circuits regulated by glutaminases. We also
show the plethora of anticancer drugs that specifically inhibit glutaminase isoenzymes for treating
several sets of cancer.
Cancer-on-a-chip for Drug Screening
Authors: Lee I-Chi
Published: April 2019

:

The oncology pharmaceutical research spent a shocking amount of money on target validation and
drug optimization in preclinical models because many oncology drugs fail during clinical trial phase III. One of
the most important reasons for oncology drug failures in clinical trials may due to the poor predictive tool of
existing preclinical models. Therefore, in cancer research and personalized medicine field, it is critical to improve
the effectiveness of preclinical predictions of the drug response of patients to therapies and to reduce costly failures
in clinical trials. Three dimensional (3D) tumor models combine micro-manufacturing technologies mimic
critical physiologic parameters present in vivo, including complex multicellular architecture with multicellular
arrangement and extracellular matrix deposition, packed 3D structures with cell–cell interactions, such as tight
junctions, barriers to mass transport of drugs, nutrients and other factors, which are similar to in vivo tumor tissues.
These systems provide a solution to mimic the physiological environment for improving predictive accuracy
in oncology drug discovery.

:

his review gives an overview of the innovations, development and limitations of different types of tumor-like
construction techniques such as self-assemble spheroid formation, spheroids formation by micro-manufacturing
technologies, micro-dissected tumor tissues and tumor organoid. Combination of 3D tumor-like construction and
microfluidic techniques to achieve tumor on a chip for in vitro tumor environment modeling and drug screening
were all included. Eventually, developmental directions and technical challenges in the research field are also
discussed. We believe tumor on chip models have provided better sufficient clinical predictive power and will
bridge the gap between proof-of-concept studies and a wider implementation within the oncology drug development
for pathophysiological applications.
Causality Assessment of Adverse Drug Reaction: Controlling Confounding Induced by Polypharmacy
Authors: Dang Tran-Thai , Nguyen Thanh-Hang , Ho Tu-Bao
Published: April 2019

Background:

Post-marketing pharmaceutical surveillance, a.k.a. pragmatic clinical trials (i.e., PCT),
plays a vital role in preventing accidents in practical treatment. The most important and difficult task in PCT is to
assess which drug causes adverse reactions (i.e., ADRs) from clinical texts. The confounding (i.e., factors cause
confusions in causality assessment) is generated by the polypharmacy (i.e., multiple drugs use), which makes
most of existing methods poor for detecting drugs that capably cause observed ADRs.

Objective:

We aim to improve the performance of detecting drug-ADR causal relations from clinical texts. To
this end, a mechanism for reducing the impact of confounding on the detecting process is needful.

Methods:

We proposed a novel model which is called the analogy-based active voting (i.e., AAV) for improving
the ability of detecting causal drug-ADR pairs, in case multiple drugs are prescribed for treating the comorbidity.
This model is inspired by the analogy principle which was proposed by Bradford Hill.

Results:

The experimental results show the improvement of recognizing causal relations between drugs and
ADRs that are confirmed by the SIDER. In addition, the proposed model is promising to detect infrequently observed
causal drug-ADR pairs when the drug is not commonly used.

Conclusion:

The proposed model demonstrates its ability for controlling the polypharmacy-induced confounding,
to improve the quality of causality assessment of ADRs. Additionally, this also shows that the analogy principle
is applicable for the assessment.
Identification of N-Substituted Triazolo-azetidines as Novel Antibacterials using pDualrep2 HTS Platform
Authors: Ivanenkov Yan A. , Yamidanov Renat S. , Osterman Ilya A. , Sergiev Petr V. , Aladinskiy Vladimir A. , Aladinskaya Anastasia V. , Terentiev Victor A. , Veselov Mark S. , Ayginin Andrey A. , Skvortsov Dmitry A. , Komarova Katerina S. , Chemeris Alexey V. , Baimiev Alexey Kh. , Sofronova Alina A. , Malyshev Alexander S. , Machulkin Alexey E. , Petrov Rostislav A. , Bezrukov Dmitry S. , Filkov Gleb I. , Puchinina Maria M. , Zainullina Liana F. , Maximova Marina A. , Zileeva Zulfiya R. , Vakhitova Yulia V. , Dontsova Olga A.
Published: April 2019

Aim and Objective:

Antibiotic resistance is a serious constraint to the development of new effective antibacterials. Therefore, the discovery of the new antibacterials remains one of the main challenges in modern medicinal chemistry. This study was undertaken to identify novel molecules with antibacterial activity.

Materials and Methods:

Using our unique double-reporter system, in-house large-scale HTS campaign was conducted for the identification of antibacterial potency of small-molecule compounds. The construction allows us to visually assess the underlying mechanism of action. After the initial HTS and rescreen procedure, luciferase assay, C14-test, determination of MIC value and PrestoBlue test were carried out.

Results:

HTS rounds and rescreen campaign have revealed the antibacterial activity of a series of Nsubstituted triazolo-azetidines and their isosteric derivatives that has not been reported previously. Primary hit-molecule demonstrated a MIC value of 12.5 µg/mL against E. coli Δ tolC with signs of translation blockage and no SOS-response. Translation inhibition (26%, luciferase assay) was achieved at high concentrations up to 160 µg/mL, while no activity was found using C14-test. The compound did not demonstrate cytotoxicity in the PrestoBlue assay against a panel of eukaryotic cells. Within a series of direct structural analogues bearing the same or bioisosteric scaffold, compound 2 was found to have an improved antibacterial potency (MIC=6.25 µg/mL) close to Erythromycin (MIC=2.5-5 µg/mL) against the same strain. In contrast to the parent hit, this compound was more active and selective, and provided a robust IP position.

Conclusion:

N-substituted triazolo-azetidine scaffold may be used as a versatile starting point for the development of novel active and selective antibacterial compounds.
Identification of DNA-Binding Proteins by Multiple Kernel Support Vector Machine and Sequence Information
Authors: Ding Yijie , Chen Feng , Guo Xiaoyi , Tang Jijun , Wu Hongjie
Published: April 2019

Background:

The DNA-binding proteins is an important process in multiple biomolecular
functions. However, the tradition experimental methods for DNA-binding proteins identification are
still time consuming and extremely expensive.

Objective:

In past several years, various computational methods have been developed to detect DNAbinding
proteins. However, most of them do not integrate multiple information.

Methods:

In this study, we propose a novel computational method to predict DNA-binding proteins by
two steps Multiple Kernel Support Vector Machine (MK-SVM) and sequence information. Firstly, we
extract several feature and construct multiple kernels. Then, multiple kernels are linear combined by
Multiple Kernel Learning (MKL). At last, a final SVM model, constructed by combined kernel, is built
to predict DNA-binding proteins.

Results:

The proposed method is tested on two benchmark data sets. Compared with other existing
method, our approach is comparable, even better than other methods on some data sets.

Conclusion:

We can conclude that MK-SVM is more suitable than common SVM, as the classifier for
DNA-binding proteins identification.
GCMS Analysis of Bioactives of Piper betle Linn. Leaf
Authors: Saini Sapna , Nanda Sanju , Dhiman Anju
Published: April 2019

Background:

Piper betle Linn. (Piperaceae) is commonly known as “Green gold of India”
due to its high commercial value. It is a Vedic plant used in various traditional system of medicine v.iz.
Ayurveda system of medicine, Yunani system of medicine attributed to various pharmacological activities
v.i.z. antioxidant, anti-inflammatory, analgesic, anti-fertility, antimutagenic etc.

Objectives:

The main objective of the present study is quantitative estimation of the various bioactive
compounds present in leaf of P. betle L. (Bangla variety) by GC-MS analysis using head space method.

Material and Methods:

The vapor sample of leaf of P. betle L. was prepared by head space method for
GCMS analysis. GCMS analysis was carried out using high resolution Thermo scientific TSQ 8000 Gas
Chromatograph-Mass Spectrometer. Helium is used as carrier gas (99.999 %) with a constant flow rate
of 1 ml/min. Total run time was 29.10 min.

Results:

GC-MS analysis revealed the presence of 16 bioactive compounds in leaf of P. betle L.
Eugenol (29.13%), Bakuchiol (11.79%) and α-linalool (14.88%) are the chief bioactive compounds of
leaf. Leaf has been also reported to contain a good percentage of various terpenoids like β-
caryophyllene (7.69), α-muurolene (4.19), α-bisabolene (0.98), etc.

Conclusion:

From GC-MS analysis results it can be concluded that leaf of P. betle L. is a reservoir of
valuable phenolic and susquiterpene compounds. Eugenol chief biomarker compound present leaf is a
versatile remarkable molecule. Due to number of phytoconstituents P. betle L. leaf is recommended as
plant of phytopharmaceutical importance.
Synthesis and Biological Evaluation of Novel 4,5,6,7-Tetrahydrobenzo[D]-Thiazol-2- Yl Derivatives Derived from Dimedone with Anti-Tumor, C-Met, Tyrosine Kinase and Pim-1 Inhibitions
Authors: Mohareb Rafat M. , Abouzied Amr S. , Abbas Nermeen S.
Published: April 2019

Background:

Dimedone and thiazole moieties are privileged scaffolds (acting as primary pharmacophores)
in many compounds that are useful to treat several diseases, mainly tropical infectious diseases. Thiazole
derivatives are a very important class of compounds due to their wide range of pharmaceutical and therapeutic
activities. On the other hand, dimedone is used to synthesize many therapeutically active compounds. Therefore,
the combination of both moieties through a single molecule to produce heterocyclic compounds will produce
excellent anticancer agents.

Objective:

The present work reports the synthesis of 47 new substances belonging to two classes of compounds:
Dimedone and thiazoles, with the purpose of developing new drugs that present high specificity for tumor
cells and low toxicity to the organism. To achieve this goal, our strategy was to synthesize a series of
4,5,6,7-tetrahydrobenzo[d]-thiazol-2-yl derivatives using the reaction of the 2-bromodimedone with cyanothioacetamide.

Methods:

The reaction of 2-bromodimedone with cyanothioacetamide gave the 4,5,6,7-tetrahydrobenzo[d]-
thiazol-2-yl derivative 4. The reactivity of compound 4 towards some chemical reagents was observed to produce
different heterocyclic derivatives.

Results:

A cytotoxic screening was performed to evaluate the performance of the new derivatives in six tumor
cell lines. Thirteen compounds were shown to be promising toward the tumor cell lines which were further
evaluated toward five tyrosine kinases.

Conclusion:

The results of antitumor screening showed that many of the tested compounds were of high inhibition
towards the tested cell lines. Compounds 6c, 8c, 11b, 11d, 13b, 14b, 15c, 15g, 21b, 21c, 20d and 21d were
the most potent compounds toward c-Met kinase and PC-3 cell line. The most promising compounds 6c, 8c, 11b,
11d, 13b, 14b, 15c, 15g, 20c, 20d, 21b, 21c and 21d were further investigated against tyrosine kinase (c-Kit, Flt-3,
VEGFR-2, EGFR, and PDGFR). Compounds 6c, 11b, 11d, 14b, 15c, and 20d were selected to examine their
Pim-1 kinase inhibition activity the results revealed that compounds 11b, 11d and 15c had high activities.
Novel Pyrazolo[3,4-d]pyrimidines as Potential Cytotoxic Agents: Design, Synthesis, Molecular Docking and CDK2 Inhibition
Authors: Maher Mai , Kassab Asmaa E. , Zaher Ashraf F. , Mahmoud Zeinab
Published: April 2019

Background:

Pyrazolo[3,4-d]pyrimidine scaffold was reported to possess potent cytotoxic and CDK2
inhibitory activity as analogue of roscovitine.

Objective:

To design and synthesize novel 1-(4-flourophenyl)pyrazolo[3,4-d]pyrimidine derivatives as bioisosters
of roscovitine with potential cytotoxic and CDK2 inhibitory activity.

Methods:

A series of novel 1-(4-flourophenyl)pyrazolo[3,4-d]pyrimidines were designed and synthesized.
Structural elucidation for all the newly synthesized compounds was achieved through performing MS, 1H NMR,
13C NMR and IR spectral techniques. Eight compounds were screened for their cytotoxic activity by National
Cancer Institute (USA) against 60 different human cancer cell lines. Compounds 2a, 4, 6, 7b, 8a and 8b were
further studied through the determination of their IC50 values against the most sensitive cell lines. The inhibitory
activities of compounds 2a and 4 were evaluated against CDK2 enzyme.

Results:

Compound 4 exhibited the most prominent broad-spectrum cytotoxic activity against 42 cell lines representing
all human cancer types showing growth inhibition percentages from 53.19 to 99.39. Compound 2a
showed promising selectivity against several cell lines. Moreover, all the test compounds exhibited potent cytotoxic
activity in nanomolar to micromolar range with IC50 values ranging from 0.58 to 8.32μM. Compound 2a
showed significant cytotoxic activity against CNS (SNB-75), lung (NCI-H460) and ovarian (OVCAR-4) cancer
cell lines with IC50 values 0.64, 0.78 and 1.9μM, respectively. Compound 4 showed promising potency against
leukemia (HL-60) and CNS (SNB-75) cell lines (IC50 = 0.58 and 0.94μM, sequentially). Moreover, the antiproliferative
activities of compounds 2a and 4 appeared to correlate well with their ability to inhibit CDK2 at
sub-micromolar level (IC50 = 0.69 and 0.67μM, respectively) that were comparable to roscovitine
(IC50=0.44μM). The Molecular docking results revealed that compound 4 interacted with the same key amino
acids as roscovitine in the active site of CDK2 enzyme with a marked docking score (-14.1031 kcal/mol).

Conclusion:

1-(4-Flourophenyl)pyrazolo[3,4-d]pyrimidine is a promising scaffold for the design and synthesis
of potent cytotoxic leads.
A Quantitative Study of the Impact of Human Competences of Distribution Logisticians on Their Intention to Leave
Authors: Fatima Ibnchahid , Said BALHADJ , Jaouad LHALLOUBI
Published: April 2019

Context:

Distribution logistics is the field of the logistics, which is a key sector at the center of economic issues and concerns of the public authorities. Distribution logistics is recognized as a powerful tool for achieving the competitiveness of a company, which is why a growing need has been observed for highly qualified employees who can handle difficult processes.

The issue of logistics skills is important for management, career development and training. Nevertheless, human skills remain an important factor in retaining employees in Moroccan boards.

Objectives:

This research thus contributes to the construction of theories in the contemporary literature on human competence, in particular on the skills necessary for distribution logisticians to succeed in their jobs and careers.

Research approach:

On a methodological level, our positivist approach in its paradigm of human skills modeling, is based on a research in a public company, which we preceded by the examination of the point of view of the managers and technicians in distribution logistics, thanks to confirmatory research.

Methods:

The study was conducted in Morocco with 95 logisticians in different autonomous administrations of water and electricity distribution. We used SMART PLS to analyze the results.

Results:

Our contribution consists of demonstrating how the two variables: social skills, decision-making skills influence the intention to leave through the mediation of individual performance.
On Application of Metonymy in Advertisements
Authors: Sun Jing , Lin Yuewu
Published: April 2019

Metonymy, as a thinking way, on the basis of contiguity, uses one object to replace another object to activate the association for them in the same Idealized Cognitive Model (ICM). In other words, metonymy stresses on the mentally transformed process from the source domain to the target domain in the same Idealized Cognitive Model (ICM). Idealized cognitive models are those structures which help conceptualizing some certain entities, events and even abstract ideas in his mind in a specific cultural background. There are some main characteristics of idealized cognitive models. The most principal and prime point is that ICMs are idealized and cultural-based. What’s more important, ICMs are embodied because of the interaction between human beings and the outside world. Last but not least, ICMs are a kind of complex gestalt structure due to the compositions of many cognitive models. Later, as the media and Internet have developed dramatically, the merchants want to use a brief way to promote their products so that there appears the advertisement. Gradually, the advertisement gets into our life and then it is a part of our life. The characteristics of advertisement are brief and clear to attract most of the consumers. It is universally known that there is an AIDA principle in advertisements. “A” means attention. “I’ is interesting. “D” shows desire. “A” refers to action. In the end, not only can we draw the conclusions that the application of metonymy in advertisement can help highlight the features of advertisements so that it makes the advertisement more vivid and interesting, but also that the advertisements containing metonymy can give the customers great impressions as well as they can attach to the final destination to help the boss sell more products.
Evaluación de la adherencia al tratamiento de los pacientes menores de 18 años con fibrosis quística en el Hospital Infantil Dr. Robert Reid Cabral de Santo Domingo, República Dominicana, durante el período febrero-abril 2018
Authors: Cordero-Oñate Leandra , García-García Yanira , Alcántara Acosta Baylin , Fernández Troncoso Gisselle Marie , George Flores Jennifer , Mercado Jhomayri , Pugibet Enrique
Published: April 2019

Introducción: la fibrosis quística es una enfermedad hereditaria autosómica recesiva que afecta las células que producen el sudor, el moco y los jugos digestivos. Esta patología afecta a 1 de cada 2.000-3.500 nacidos vivos, siendo la más frecuente de las enfermedades hereditarias mortales en la población de raza blanca.

Objetivo: evaluar la adherencia al tratamiento de los pacientes menores de 18 años con fibrosis quística en el Hospital Infantil Dr Robert Reid Cabral de Santo Domingo, República Dominicana, durante el período febrero - abril 2018.

Material y métodos: estudio prospectivo, descriptivo y de corte transversal en el que se realizaron cuestionarios a 32 guardianes de pacientes del servicio de neumología pediátrica con fibrosis quística de un hospital.

Resultados: el nivel de adherencia al tratamiento fue bajo, representado por un 41%. La principal razón de no adherencia al tratamiento fue el alto costo monetario con un 61%. Con respecto a la adherencia y nivel socioeconómico, se mostró que el nivel socioeconómico más frecuente fue el D+, el cual se encuentra en la mitad baja de la escala y la mayoría de pacientes eran no adherentes. La vía de administración de tratamiento preferida fue la oral con un 78%. La mayoría de los pacientes tienen un nivel bajo de conocimiento del tratamiento, representado por un 32%.

Conclusiones: los pacientes contaban con una baja adherencia al tratamiento, debido al alto costo monetario de los medicamentos. La mayoría de ellos preferían tratamientos administrados por vía oral, pertenecían al nivel socioeconómico D+ y contaban con un conocimiento bajo acerca del tratamiento.
Evaluación del consumo de antibióticos con y sin prescripción médica en infantes menores de cinco años en la comunidad de Paya en medio-Baní, durante el período agosto-octubre 2018
Authors: Almonte Quiñones Cristhian J. , Martínez Jiménez Penélope , Valdez Aquino Cástulo J. , Contreras Cristina , López Emilton
Published: April 2019

Introducción: la resistencia antibiótica se está perfilando como una grave amenaza en todo el mundo. Cada día están propagándose a nivel mundial nuevos mecanismos de resistencia que ponen en peligro nuestra capacidad para tratar las enfermedades infecciosas.

Objetivo: evaluar el consumo de antibióticos con y sin prescripción médica de los niños menores de 5 años en la comunidad de Paya en medio-Baní, durante el periodo agosto-octubre 2018.

Material y métodos: estudio prospectivo, descriptivo, observacional, de corte transversal con una muestra de 68 niños menores de 5 años.

Resultados: se determinó que el 46% de la muestra utilizó antibióticos en los últimos 3 meses. El 68% de los infantes que consumieron antibióticos lo obtuvieron bajo prescripción médica y el 32% lo obtuvieron por venta libre. El antibiótico de mayor consumo fue la Amoxicilina. El motivo que mayor uso de antibiótico requirió fue relacionado a síntomas del aparato respiratorio.

Conclusiones: en un corto período de tiempo de tan solo 3 meses, menores de 5 años fueron altamente expuestos a antibióticos. Debido a que su uso puede contribuir a la resistencia bacteriana, estos resultados tienen implicaciones importantes para la educación en salud pública y para la normativa de ventas de antibióticos en la República Dominicana.
Intoxicación pediátrica por Paraquat: presentación de caso, Hospital Infantil Dr. Robert Reid Cabral (HIRRC), República Dominicana
Authors: Contreras Cristina , Corona Lisandra , Domínguez Norys , Mejía Sang Miguel
Published: April 2019

Introducción: el paraquat (dicloruro de 1,1’-dimetil-4,4’-bipiridilo) es un químico tóxico utilizado mundialmente como herbicida. La etiología de la intoxicación por paraquat se debe en su mayoría a la ingestión suicida. Fisiopatológicamente, el pulmón, el hígado y el riñón son los órganos especialmente afectados por el paraquat debido a su alta saturación de oxígeno. La letalidad por intoxicación por paraquat es muy alta, cerca del 80%, siendo la fibrosis pulmonar la causa más frecuente de muerte.

Objetivos: el siguiente documento tiene la intención de identificar los principales signos y síntomas que constituyen la presentación clínica de la intoxicación por paraquat en un paciente pediátrico. A pesar de la limitada bibliografía, se pretendió hacer una comparación de la presentación y el progreso clínico con otros casos de intoxicación por paraquat.

Material y Métodos: se expone el caso clínico de una joven de 15 años, la cual ingirió aproximadamente 250 ml de paraquat con intención autolítica. La misma presentó inicialmente afección en el tracto gastrointestinal, seguido de alteración de las pruebas hepáticas, renales y pancreáticas, y dificultad respiratoria.

Conclusiones: luego de describir la presentación clínica de nuestra paciente, y de compararla con la de otros casos similares, concluimos que el diagnóstico precoz y exhaustivo de la intoxicación por paraquat reduce significativamente su mortalidad debido a la actual inexistencia de un antídoto específico para dicho envenenamiento. Asimismo, recomendamos tener siempre presente la intoxicación por paraquat al momento de recibir pacientes con la presentación característica luego de la ingesta de sustancias desconocidas.
Características clínico epidemiológicas de los casos de tuberculosis infantil diagnosticados en el Hospital Infantil Dr. Robert Reid Cabral 2007- 2012
Authors: Melgen Ricardo Elías , Pantaleón Elsa Camilo , Lazala J Adria , Abreu B. Aimee
Published: April 2019

Introducción: la TB infantil es considerada como un evento centinela de transmisión de la enfermedad y su control no ha sido una prioridad. República Dominicana es un país de elevada incidencia de TB y los datos reportados de TB infantil apuntan a una baja detección de casos y un sub diagnóstico.

Objetivo: conocer las características clínico epidemiológicas de los casos de Tuberculosis infantil diagnosticados en el Hospital Infantil Dr. Robert Reid Cabral. 2007- 2012.

Métodos: estudio descriptivo. Se revisaron los expedientes clínicos de los pacientes menores de 15 años de edad diagnosticados y seguidos en el programa de tuberculosis infantil del Hospital Infantil Robert Reid Cabral en los años 2007-2012.

Resultados: se evaluaron 249 pacientes, 81% con diagnóstico de TB infantil y 19% con Infección Latente por Tuberculosis. 56.2% tenía menos de 5 años de edad y 61.4% tenían cicatriz de vacuna BCG. 63.5% de los casos refería el contacto con un caso de Tuberculosis, y en el 70.0% era intradomiciliario. La manifestación pulmonar representó el 63.4% de los casos TB y el 27.3% tenían baciloscopia positiva.

Conclusión: existen diferencias importantes en el diagnóstico y manejo de la tuberculosis infantil y la TB en el adultos. Estas deben ser considerados en la implementación de estrategias para el control; con acciones integradas, un enfoque orientado no solo al niño, sino también a la familia y tomando en consideración los determinantes sociales
Validation of Sterile Water for Injection In Pharmaceutical Industry and Othersterile Facility
Authors: Choudhary Neetu , Patil Bhagyashri , Choukse Raju , Varma Ajit Kumar , Bairagee Deepika , Kulkarni Sweta
Published: April 2019

Sterile facilities for all pharmaceutical product specially to parentral preparation, is a must important back bone of sterile formulation andor pharmaceutical dosage form. There is most important to sterile of the areas where the formulation process proceed from initial to final stage. The sterile injectable products are very critical and sensitive products as they are administered directly into blood circulation. These products are designed such that it should be free from micro-organisms, pyrogens and unacceptable particulate matter. Any failure in quality and purity of these products may directly affect the safety of patient being treated. FDA, WHO, ISO and Good Manufacturing Practiceshas established the guides to the development of sterile pharmaceutical preparation facilities for health care establishments.This report covers all summaries that the three batches of Methylcobalamine injection 2 ml have been validated with the support of process validation protocol.
Integração do Google Drive e WhatsApp como ambiente de aprendizagem em uma disciplina no ensino superior
Authors: Santos Zenildo , Sant'ana Claudinei De Carmargo
Published: April 2019

Este artigo apresenta uma pesquisa realizada com estudantes matriculados no quarto semestre do curso de Licenciatura em Matemática da Universidade Estadual do Sudoeste da Bahia (UESB). Trata-se de uma abordagem qualitativa, que tem como objetivo analisar as potencialidades do Google Drive e o WhatsApp como ambiente virtual de aprendizagem. Para isso, utiliza o processo de interação síncrona e assíncrona dos estudantes levando em consideração as discussões e comentários dos textos no grupo do WhatsApp e das atividades postadas no Google Drive. Utiliza-se da Análise de Conteúdo para tratar os dados levantados. Os resultados apontam que tanto o uso do WhatsApp quanto o Google Drive favoreceram a interação, cooperação e colaboração dos discentes no processo de ensino-aprendizagem para além dos limites físicos da sala de aula.