bluuebunny/crossref_metadata_2025 huggingface dataset:
Accumulation and antigenicity of truncated porcine circovirus type 2 capsid protein in Escherichia coli cells
Authors: Titova Anastasia D. , Kuleshova Yuliya M. , Potapovich Maxim I. , Prokulevich Vladimir A.
Published: July 2022

This work examines accumulation of shortened version of porcine circovirus type 2 capsid protein (SOP protein) in the cells of bacterial strain Escherichia coli BL21-CodonPlus(DE3)-RIPL with plasmid pET-SOP, obtained in previous work. The gene for SOP protein was modified by deletion of the part (108 base pair) interfering expression in procaryotes, as well as optimisation of 93 rare codons. Under cultivation temperature 37 °C for 2 h after induction the proportion of target protein reaches of 24 % of the total cellular protein, which makes it possible to classify this strain as an effective industrial producer of target protein. During the cultivation of the producer at 37 °C, the target protein is in the cells in the native soluble form right after induction, but 1 h after the addition of the inducer, it is found mostly in insoluble multimeric form (inclusion bodies). When the cultivation temperature is lowered to 18–30 °C, the formation of inclusion bodies slows down, however the proportion of recombinant protein in the cells of the producer decreases to 15– 6 % respectively, which significantly reduces the profitability of the technological process. It has been established that the modified recombinant SOP protein obtained from bacterial cells of the producer strain retains its antigenic activity, which is confirmed by specific enzyme-linked immunosorbent assay analysis. These data allow us to consider studied protein as a promising candidate for a porcine circovirus type 2 vaccine.
Stress-induced electrolyte leakage from root cells of higher plants: background, mechanism and physiological role
Authors: Hryvusevich Palina V. , Samokhina Veranika V. , Demidchik Vadim V.
Published: July 2022

Electrolyte leakage from tissues is one of the central reactions of the plant organism to stress. It is observed under almost any type of stresses, both abiotic and biotic. The loss of key electrolytes can lead to significant changes in metabolism and, in some cases, to the death of cells or the whole organism. For a long time, it was believed, that electrolyte leakage is associated with disruption of cell integrity and plasma membranes degradation, and that it is an unregulated process. However, in recent years, a lot of evidence has been received that, in most cases, electrolyte leakage is inhibited by ion channel blockers and reversible. It means that it is associated with the transfer of ions through the membrane by transport proteins, such as ion channels. Recently, the experimental evidence has been obtained, that under salinity, drought, pathogen attack, excessive levels of heavy metals, hypo- and hyperthermia, as well as oxidative stress, the electrolyte leakage in plant cells is mediated by several types of cation and anion channels, including K+-selective channels (SKOR and GORK), anion channels (such as ALMT1) and a number of non-selective cation channels. It has been demonstrated that the primary reactions that induce electrolyte leakage are plasma membrane depolarisation and generation of reactive oxygen species, leading to the activation of redox-regulated outwardly rectifying K+ channels, such as SKOR and GORK. Potassium efflux is up-stream and stimulates the counterion flow (transport of anions) through the anion channels. The regulation of electrolyte leakage at the ion channel level and the corresponding selection for ion channel properties can become an important link in the directed control of stress resistance in higher plants. This can be applied in agriculture via breeding of stress-tolerant plant varieties, as well as developing modern amelioration techniques.
Dynamics of gut microbiota in patients suffering from hematologic malignancies after allogeneic hematopoi-etic stem cell transplantation
Authors: Akhremchuk Katsiaryna V. , Skapavets Katsiaryna Y. , Akhremchuk Artur E. , Kirsanava Natallia , Sidarenka Anastasiya V. , Valentovich Leonid N.
Published: July 2022

In this study, we described the dynamics of intestinal microbiota of 32 patients after allogeneic hematopoietic stem cell transplantation (HSCT). After HSCT, on 14th day and 30th day, the inverse Simpson index value was more than 3 times lower compared to the pre-HSCT period. Biodiversity became more similar to the pre-HSCT level by 100th day. The correlation between diversity of microbiota and development of post-HSCT complications wasn’t observed. Our findings indicated individual-specific changes in the taxonomic structure of patients’ microbiota after HSCT. Changes in the relative abundance of the bacterial families Streptococcaceae, Actinomycetaceae, Acidaminococcaceae, Akkermansiaceae, Desulfovibrionaceae, Prevotellaceae, genera Streptococcus, Clostridioides, Phascolarctobacterium, Paraprevotella, Bilophila, Akkermansia, Coprococcus, Sutterella, Alistipes appear to be associated with the development of post-HSCT complications. High abundance of phylum Proteobacteria, families Actinomycetaceae and Streptococcaceae, genus Streptococcus before HSCT can be considered as a potential predictor of graft-versus-host disease development in the post-HSCT period.
Characteristic of strains Priestia megaterium Ср-1 and Rhodococcus jostii СА-6 demonstrating increased salt resistance
Authors: Naumovich Nadezhda I. , Aleschenkova Zinaida M. , Ananyeva Irina N. , Safronava Halina V.
Published: July 2022

The strains Priestia megaterium Ср-1 and Rhodococcus jostii CA-6 were shown to preserve phosphate-solubilising ability, nitrogen-fixing activity and synthesis of indole-3-acetic acid under osmotic stress conditions caused by sodium chloride in concentration range of 1197–2052 mmol/L, as well as to promote seed germination rate and development of small radish seedlings. Proline, betaine and sucrose were found to exert a beneficial effect on growth and ability of strains P. megaterium Ср-1 and Rh. jostii CA-6 to adapt to osmotic stress. Mass spectrometric analysis has revealed that intracellular synthesis of proline and betaine in both bacteria was directly proportional to sodium chloride concentration: rising NaCl level in the medium to 1197 mmol/L was accompanied by increase in proline level from 0.062 to 1.502 µg /mL (Rh. jostii CA-6) and from 0.089 to 2.105 µg /mL (P. megaterium Ср-1), in a similar manner betaine production grew up from 0.071 to 0.118 µg /mL (Rh. jostii СА-6) and from 0.055 to 0.219 µg /mL (P. megaterium Ср-1).
Pollen cargo structure on Aculeata – visitors of alien Solidago canadensis L. s. l. inflorescences in Belarus
Authors: Koroteeva Daria O. , Sheiko Anna A.
Published: July 2022

Community structure of visitors to inflorescences of invasive goldenrods was investigated by sampling at 9 sites in Minsk city, Minsk and Mogilev regions in Belarus. A total 44 species of Aculeata including 1 species of Chrysioidea, 7 species of Vespoidea and 36 species of Apoidea were registered. Among them 2 species of bumblebees (Bombus terrestris L., B. lapidarius L.) were numerous, 2 species of Vespoidea (Polistes dominula Christ, P. nimpha Christ) and 3 species of Apoidea (Philanthus triangulum F., Hylaeus communis Nyl., B. ruderarius Müller) were common. Pollen cargo analysis revealed the predominance of easily lost and relatively easily lost conspecific pollen grains on the bodies of the imago of the all common and numerous species of visitors to inflorescences of invasive goldenrods.
Gas chromatography – mass spectrometry analysis of acetone extract of Lactuca tatarica (L.) C. A. Mey. roots
Authors: Shukurlu Emil Namik
Published: July 2022

Research work is devoted to the chemical investigation of the roots of the Lactuca tatarica (L.) C. A. Mey. by gas chromatography – mass spectrometry method. From the crude extract that is obtained from the roots, the presence of natural compounds of various groups has been detected. As a result, 25 different substances including acyclic compounds (methyl myristate, palmitic acid methyl ester, ethyl palmitate, methyl elaidate, methyl stearate, ethyl oleate, etc.) have been identified from the solid mass of the research object. The most abundant fatty acid esters are methyl oleate (6.831 %) and palmitic acid methyl ester (2.902 %). In addition, the triterpenoid compounds (lupeol acetate, cycloartenol acetate, 3β,13β,14β-13, 27-cycloursan-3-ol acetate) which are characteristic of the L. tatarica have been identified. Lupeol acetate is the predominant component which makes up 56.35 % of the whole obtained substances. Elaidic acid which belongs to the fatty acids group, bis(2-ethylhexyl)phthalate which belongs to the aromatic dicarboxylic acid esters and other compounds have also been identified. Among the identified substances, some are found previously from the other species of the genus Lactuca L. but with regards to the L. tatarica to the best of our knowledge the majority of them are firstly being reported in this study. The investigated plant is widespread in the lowlands and coastal areas of Azerbaijani flora. The availability of some pharmacological effective compounds (lupeol acetate, 3β,13β,14β-13, 27-cycloursan-3-ol acetate, olean-12-en-3-yl acetate) makes the research significant in terms of chemical aspect. Therefore, the results of this investigation open up new horizons for future studies in various fields including the pharmaceutical one.
EVALUASI KARAKTERISTIK LALU LINTAS DAN TINGKAT PELAYANAN JALAN DI KAWASAN SIMPANG KECAMATAN PARUNG KABUPATEN BOGOR
Authors: NGURAH PURNAMA JAYA GDE
Published: July 2022

Persimpangan Parung merupakan ruas jalan kolektor sekunder yang menghubungkan antara Kecamatan Parung dengan wilayah Kota Bogor, Ciseeng, Tangerang, Ciputat dan Kota Depok serta merupakan perpotongan dari ruas Jalan Raya Parung - Bogor dan Jalan Haji Mawi-Ciseeng, Jalan Raya Gunung Sindur dan Jalan Raya Sawangan. Selain itu, kawasan simpang Parung juga merupakan salah satu kawasan yang memiliki permasalahan transportasi yang cukup komplek, seperti kondisi jalanan padat, terjadinya perlambatan pergerakan kendaraan, kemacetan hampir terjadi disepanjang ruas jalan, serta kenyamanan berlalu-lintas berkurang akibat tingginya hambatan samping. Berdasarkan hasil identifikasi kondisi kegiatan sekitar sepanjang ruas jalan dan analisis karakteristik arus lalu lintas kendaraan di kawasan simpang Parung Parung,ditemukan bahwa:  Penyebab permasalahan Lalu Lintas yang terjadi di Kawasan Simpang Parung : tingginya hambatan samping,  terjadinya penyempitan badan jalan yang diakibatkan oleh banyaknya angkutan kota dan angkutan bis yang ngetem, manajemen lalu lintas kawasan simpang belum tertata dengan baik,.Karakteristik Lalu Lintas di Ruas Jalan Kawasan Simpang Parung: bahwa kapasitas dasar masing-masing ruas jalan lebih tinggi dari kapasitas aktual dari masing-masing ruas jalan kawasan simpang di Kecamatan Parung, besarnya volume lalu lintas kendaraan dari masing-masing ruas jalan pada saat jam-jam puncak. Sehubungan dengan keadaan dan permasalahan lalu-lintas yang terjadi di kawasan simpang Parung, maka upaya yang dapat dilakukan untuk mengatasi kemacetan lalu-lintas yang terjadi adalah dengan cara perbaikan manajemen lalu-lintas yang tujuannya meningkatkan atau memperbaiki kinerja ruas jalan, sehingga nantinya diharapkan dapat melayani arus lalu-lintas dengan baik dan lancar. Kata Kunci: Perkotaan, Transport, Volume Lalu Lintas
KAJIAN PENINGKATAN TEGANGAN IJIN LEMPUNG LUNAK DI LAHAN BANGUNAN KOTA CINEMA MALL BANJARMASIN
Authors: LUKMAN HIKMAD
Published: July 2022

Sebagian besar lapisan tanah di pulau Kalimantan dan pulau Sumatera, terutama di pesisir pantai berupa tanah gambut terdiri dari lempung lunak dan hamparan lahan gambut yang berasal dari humus tumbuh-tumbuhan dengan ketebalan yang cukup besar. Sifat tanah gambut  mempunyai kompresibilitas yang besar, gaya geser yang rendah, koefisien permeabilitas yang rendah dan mempunyai daya dukung yang rendah. Pada kajian ini, penelitian dilakukan pada lahan dimana akan dibangun bangunan Kota Cinema Mall Banjarmasin di Jalan Belitung Banjarmasin Provinsi Kalimantan Selatan. Hasil tegangan konus pada percobaan Sondir yang diperoleh sampai kedalaman 30 m bernilai kecil ( 1 - 15 kg/cm²) yang mencirikan bahwa lapisan tanah tersebut adalah lembek. Berdasarkan hasil perhitungan tegangan ijin menggunakan metoda De Beer, untuk kedalaman 1,5 m diperoleh besarnya tegangan ijin tanah pondasi Dangkal hanya sebesar 0,084 kg/cm². Untuk meningkatkan besarnya daya dukung, maka diperlukan metoda perbaikan tanah dengan menggunakan cerucuk dari kayu dolken. Berdasarkan hasil perhitungan besarnya daya dukung pondasi dalam untuk kumpulan tiang dolken diameter 10 cm dengan panjang bervariasi 7,5 – 9,0 m diperoleh besarnya daya dukung kombinasi pondasi Dangkal dan pondasi Dalam terjadi adanya peningkatan besarnya daya dukung menjadi sebesar 0,42 kg/cm². Besarnya tegangan ijin tanah kombinasi pondasi Dangkal dan penggunaan tiang dolken meningkat menjadi sebesar 0,51 kg/cm2. Selanjutnya berdasarkan perhitungan besarnya penurunan tanah akibat penurunan seketika dan penurunan akibat konsolidasi dengan beban luar 3,5 t/m2 diperoleh sebesar 71 cm untuk beban sekaligus atau 36 cm untuk asumsi beban bekerja secara lambat laun. Kata kunci: lempung lunak, tanah gambut, dolken, tegangan ijin tanah
ANALISIS SEBARAN KEGIATAN PERTAMBANGAN TIMAH MENGGUNAKAN SISTEM INFORMASI GEOGRAFI DI DAERAH BANGKA, PROPINSI BANGKA BELITUNG
Authors: Bambang Sunarwan Mustafa Luthfi,
Published: July 2022

AbstrakKawasan Pulau Bangka telah sejak lama dikenal sebagai wilayah penghasil timah. Dan khusus Kabupaten Bangka menghasilkan logam timah terbanyak di P Bangka atau sekitar 7,3 juta Mton pada tahun 2006. Kemudian diikuti Kabupaten Bangka Selatan dengan 6,6 juta Mton. Akan tetapi secara kebetulan diketahui sebagian besar bijih tersebut dihasilkan dari proses pertambangan tanpa ijin, dimana kegiatan ini bila dibiarkan akan mengakibatkan dampak buruk terhadap lingkungan. Untuk Kabupaten Bangka, Kecamatan Belinyu merupakan kecamatan penghasil timah terbanyak, yaitu 1,95 juta Mton, diikuti Kecamatan Riau Silip dengan 1,66 juta Mton.Kolong atau kolam yang terbentuk dalam proses pertambangan di wilayah terbuka merupakan karakteristik pada wilayah tersebut. Karakter tersebut dapat dengan mudah diidentifikasi melalui citra satelit. Sebaran kolong timah yang diperoleh kemudian dioverlaykan dengan sebaran tutupan lahan, sebaran kuasa pertambangan timah dan sebaran batuan. Overlay dilakukan dengan menggunakan Program Arc View 3.3.Kolong penambangan terdapat terdapat pada semua jenis tutupan lahan dan terbanyak dilakukan pada tutupan lahan semak dan ruang terbuka, dengan jumlah kolong total 412. dan terbanyak terdapat di wilayah Kecamatan Belinyu dengan jumlah 230. Sebaran pertambangan berada pada batuan granit dan endapan aluvial.Dalam rangka mengurangi dampak kegiatan tambang timah, maka Pemda perlu melakukan langkah-langkah terkait dengan penataan tata ruang daerah dan untuk ini telah bekerja-sama dengan Dinas Pertambangan melakukan pengawasan terhadap kegiatan pertambangan. Evaluasi perijinan yang ada diberlakukan, agar mampu menghasilkan peraturan pertambangan memiliki peran dan arti secara terpadu.Kata-kata kunci : Kolong, overlay, inkonstitusional, kontribusi., signifikan, spesifik. interpretasi citra satelit, PETI, inkonstitusional, finansia,l Pendulangan/ pelimbangan, editing, labelisasi
Malproduction of endogenous hydrogen gas in COVID-19
Authors: Ostojic Sergej M.
Published: July 2022

The molecular footprints of COVID-19 occur everywhere, even reaching the family of biologically active gases and gasotransmitters. Besides nitric oxide and hydrogen sulfide, COVID-19 might also alter the homeostasis of dihydrogen (H2), another gaseous bioactive molecule produced endogenously by the human gut bacteria. Many studies have shown various alterations of the gut microbiota in patients with coronavirus disease 2019, including the lower abundance of hydrogen-producing bacteria that could instigate the shortage of hydrogen output. Since dihydrogen has many important bioactivities, including cytoprotective, antioxidant, anti-inflammatory, and antiapoptotic, its malproduction in COVID-19 might contribute to the disease progression and severity. On the other hand, replenishing dihydrogen by exogenous administration could be beneficial in COVID-19 for both patient- and clinical-reported outcomes. Assessing low dihydrogen along with H2 supplementation to restore normal levels could be thus combined via theranostic approaches to aid COVID-19 diagnosis and treatment.
An intensive, structured, mobile devices-based healthcare intervention to optimize the lipid-lowering therapy improves lipid control after an acute coronary syndrome
Authors: Ruiz-Bustillo Sonia , Badosa Neus , Cabrera-Aguilera Ignacio , Ivern Consol , Llagostera Marc , Mojón Diana , Vicente Miren , Ribas Núria , Recasens Lluis , Martí-Almor Julio , Cladellas Mercè , Farré Núria
Published: July 2022

Aims

Despite the evidence, lipid-lowering treatment (LLT) in secondary prevention remains insufficient, and a low percentage of patients achieve the recommended LDL cholesterol (LDLc) levels by the guidelines. We aimed to evaluate the efficacy of an intensive, mobile devices-based healthcare lipid-lowering intervention after hospital discharge in patients hospitalized for acute coronary syndrome (ACS).

Methods and results

Ambiespective register in which a mobile devices-based healthcare intervention including periodic follow-up, serial lipid level controls, and optimization of lipid-lowering therapy, if appropriate, was assessed in terms of serum lipid-level control at 12 weeks after discharge. A total of 497 patients, of which 462 (93%) correctly adhered to the optimization protocol, were included in the analysis. At the end of the optimization period, 327 (70.7%) patients had LDLc levels ≤ 70 mg/dL. 40% of patients in the LDLc ≤ 70 mg/dL group were upgraded to very-high intensity lipid-lowering ability therapy vs. 60.7% in the LDLc > 70 mg/dL group, p < 0.001. Overall, 38.5% of patients had at least a change in their LLT. Side effects were relatively infrequent (10.7%). At 1-year follow-up, LDLc levels were measured by the primary care physician in 342 (68.8%) of the whole cohort of 497 patients. In this group, 71.1% of patients had LDLc levels ≤ 70 mg/dL.

Conclusion

An intensive, structured, mobile devices-based healthcare intervention after an ACS is associated with more than 70% of patients reaching the LDLc levels recommended by the clinical guidelines. In patients with LDLc measured at 1-year follow-up, 71.1% had LDLc levels ≤ 70 mg/dL.
Proteomic profiling of thyroid tissue in patients with obesity and benign diffuse goiter
Authors: Benabdelkamel Hicham , Rafiullah Mohamed , Masood Afshan , Alsaif Abdulaziz , Musambil Mohthash , Alfadda Assim A.
Published: July 2022

Goiter is a term to describe the enlargement of the thyroid gland. The pathophysiology and molecular changes behind development of diffuse benign goiter remains unclear. The present study targeted to identify and describe the alterations in the thyroid tissue proteome from patients (obese euthyroid) with benign diffuse goiter (BDG) using proteomics approach. Thyroid tissue samples, from 7 age and sex matched, patients with BDG and 7 controls were obtained at the time of surgery. An untargeted proteomic analysis of the thyroid tissue was performed out utilizing two-dimensional difference (2D-DIGE) in gel electrophoresis followed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) for identification of the proteins. Progenesis software was used to identify changes in expression of tissue proteins and found statistically significant differences in abundance in a total of 90 proteins, 46 up and 44 down (1.5-fold change, ANOVA, p ≤ 0.05) in BDG compared to the control group. Bioinformatic analysis using Ingenuity Pathway Analysis (IPA) identified dysregulation of signalling pathways linked to ERK1/2, Glutathione peroxidase and NADPH oxidase associated to organismal injury and abnormalities, endocrine system disorders and cancer. The thyroid tissue proteome in patients with BDG revealed a significant decrease in thyroglobulin along with dysregulation of glycolysis and an increase in prooxidant peroxidase enzymes. Dysregulation of metabolic pathways related to glycolysis, redox proteins, and the proteins associated with maintaining the cytoskeletal structure of the thyrocytes was also identified.
Identical Ciguatoxin-3C group profiles in Lutjanus bohar from the Pacific and Indian Oceans - indicating the need to re-evaluate geographical CTX classifications
Authors: Spielmeyer Astrid , Loeffler Christopher R. , Kappenstein Oliver
Published: July 2022

Ciguatoxins (CTXs) are a group of marine biotoxins, consisting of over 30 different congeners that can contaminate marine food webs. Generally, the molecular structures among the known congeners are variable by geographical region (i.e., ocean basin) and vector species. Limited information is available regarding the CTX profiles among or within CTX vector species and their capture regions. Within this study, an in-depth investigation based on a semi-targeted LC-MS/MS approach was conducted to investigate 52 tissue samples from a single species Lutjanus bohar (Lutjanidae), a common CTX vector, sourced from two distinct regions (Indian Ocean and Pacific Ocean). All samples revealed the presence of a complex CTX contaminant profile, with samples containing several congeners of the CTX3C-group (2,3,51-trihydroxyCTX3C, 2,3,-dihydroxyCTX3C, 2-hydroxyCTX3C, M-seco-CTX3C, 51-hydroxyCTX3C, CTX3C, and respective 49-epimers in most cases). All samples were previously found to possess a CTX-like toxicity within an in vitro cytotoxicity assay (N2a-bioassay), demonstrating the relevance of CTX3C-group congeners with regard to ciguatera poisoning. Individual samples contained an indistinguishable toxin profile within the species and among the distinct oceanic capture regions. These findings imply either a species-specific CTX metabolism or the emergence of an interoceanic CTX toxin profile. The inter-regional CTX profile demonstrated here provides further evidence that classifying CTX congeners based on ocean basins may be imprecise.
Increase in newly diagnosed type 1 diabetes and serological evidence of recent SARS-CoV-2 infection: Is there a connection?
Authors: Denina Marco , Trada Michela , Tinti Davide , Funiciello Elisa , Novara Chiara , Moretto Martina , Rosati Sergio , Garazzino Silvia , Bondone Claudia , De Sanctis Luisa
Published: July 2022

Several studies have investigated the correlation between the COVID-19 pandemic and the onset of type 1 diabetes (T1D) in children, reporting an increased incidence of T1D and severe diabetic ketoacidosis (DKA). This study aimed to investigate the infection by SARS-CoV-2 in children with newly-diagnosed T1D to explore a possible link between SARS-CoV-2 infection, T1D and DKA. Thirty-nine children with a T1D new onset between October 15, 2020, and April 15, 2021, were enrolled. SARS-CoV-2 infection was investigated through a polymerase chain reaction on the nasal swab, dosage of specific antibodies, and an anamnestic question form. Nine (23%) of them had antibodies directed toward SARS-CoV-2, and five (12%) had a history of recent SARS-CoV-2 infection in themselves or in their family. No molecular swabs were positive. Compared to the general pediatric population, the overall incidence of COVID-19 was 5.6 times higher in the T1D patients' group (p < 0.00001). Referring only to the cases in the metropolitan area, we find a net increase in the incidence of T1D compared to the 5 years preceding our study, by 50% compared to the same months in 2016/2017 and 2017/2018, by 69% compared to 2018/2019 and by 77% compared to 2019/2020. The same trend was observed regarding DKA cases. The attributable risk of the pandemic cohort compared to the previous year is 44%. The abnormal disproportion of SARS-CoV-2 infection between children with T1D and the pediatric reference population, with a ratio of 5.6, appears to support the causative role of SARS-CoV-2 in triggering the immune response underlying diabetes, as often described for other viral infections. The difficulty accessing care services during the pandemic, with a consequent diagnosis delay, does not justify the increase in observed T1D cases, which could to be directly linked to the pandemic. The acceleration of the immune process provoked by SARS-CoV-2 may play a suggestive role in the development of T1D with DKA. Multicenter studies are needed to deepen and fully understand the pathophysiological link between SARS-CoV-2 and the onset of T1D in children.
NF-κB and AP-1 are required for the lipopolysaccharide-induced expression of MCP-1, CXCL1, and Cx43 in cultured rat dorsal spinal cord astrocytes
Authors: Lu Ying , Li Bo , Xu Axiang , Liang Xuan , Xu Tao , Jin Huan , Xie Ye , Wang Rong , Liu Xiaohong , Gao Xiaohong , Han Yong , Zeng Junwei
Published: July 2022

TLR4 and Cx43 signaling in dorsal spinal cord has been shown to be involved in the development of neuropathic pain. However, it is not clear whether TLR4 signaling is associated with the expression of MCP-1, CXCL1, and Cx43 in LPS (lipopolysaccharide)-treated rat dorsal spinal cord astrocytes under in vitro condition. In the present study, we found that TLR4 antagonist TAK-242 significantly inhibited LPS-induced MCP-1, CXCL1, and Cx43 expression, suggesting the role of TLR4 in response to LPS in cultured dorsal spinal cord astrocytes. Application of TAK-242 significantly blocked LPS-induced NF-κB and AP-1 activity and the expression of MCP-1, CXCL1 and Cx43. Furthermore, NF-κB inhibitor PDTC and AP-1 inhibitor SR11302 significantly blocked LPS-induced MCP-1, CXCL1, and Cx43 expression. DNA-binding activity of NF-κB, its effect on MCP-1 expression was suppressed by PDTC and SR11302. On the other hand, DNA-binding activity of AP-1, its effect on CXCL1 or Cx43 expression was also suppressed by PDTC and SR11302. In addition, PDTC was found to inhibit the nuclear translocation of AP-1 and the expression of c-Jun induced by LPS, which suggested that NF-κBp65 is essential for the AP-1 activity. Similarly, SR11302 significantly blocked LPS-induced the nuclear translocation of NF-κBp65 and the expression of NF-κBp65 induced by LPS. Pretreatment with CBX, Gap26, or Gap19 (Cx43 blockers) significantly inhibited abnormal astrocytic hemichannel opening and chemokines (MCP-1 and CXCL1) release in LPS-stimulated astrocytes. In summary, cell culture experiments revealed that LPS stimulation could evoke TLR4 signaling with the subsequent activation of NF-κB and AP-1, resulting in the expression of MCP-1, CXCL1, and Cx43. TLR4 activation increased Cx43 hemichannel, but not gap-junction activities and induced the release of the MCP-1 and CXCL1 from astrocytes via Cx43 hemichannel. These findings may help us to understand the role of astrocytic signaling in inflammatory response within dorsal spinal cord tissue.
Association between metabolic body composition status and vitamin D deficiency: A cross-sectional study
Authors: Chen Yi-Chuan , Li Wen-Cheng , Ke Pin-Hsuan , Chen I-Chun , Yu Wei , Huang Hsiung-Ying , Xiong Xue-Jie , Chen Jau-Yuan
Published: July 2022

This study aimed to investigate the risk of vitamin D deficiency in a relatively healthy Asian population, with (i) metabolically healthy normal weight (MHNW) (homeostasis model assessment-insulin resistance [HOMA-IR] < 2. 5 without metabolic syndrome [MS], body mass index [BMI] < 25), (ii) metabolically healthy obesity (MHO) (HOMA-IR < 2.5, without MS, BMI ≥ 25), (iii) metabolically unhealthy normal weight (MUNW) (HOMA-IR ≥ 2.5, or with MS, BMI < 25), and (iv) metabolically unhealthy obesity (MUO) (HOMA-IR ≥ 2.5, or with MS, BMI ≥ 25) stratified by age and sex. This cross-sectional study involved 6,655 participants aged ≥ 18 years who underwent health checkups between 2013 and 2016 at the Chang Gung Memorial Hospital. Cardiometabolic and inflammatory markers including anthropometric variables, glycemic indices, lipid profiles, high-sensitivity C-reactive protein (hs-CRP), and serum 25-hydroxy vitamin D levels, were retrospectively investigated. Compared to the MHNW group, the MHO group showed a higher odds ratio (OR) [1.35, 95% confidence interval (CI) 1.05–1.73] for vitamin D deficiency in men aged < 50 years. By contrast, in men aged > 50 years, the risk of vitamin D deficiency was higher in the MUO group (OR 1.44, 95% CI 1.05–1.97). Among women aged < and ≥ 50 years, the MUO group demonstrated the highest risk for vitamin D deficiency, OR 2.33 vs. 1.54, respectively. Our study revealed that in women of all ages and men aged > 50 years, MUO is associated with vitamin D deficiency and elevated levels of metabolic biomarkers. Among men aged < 50 years, MHO had the highest OR for vitamin D deficiency.
Comprehensive evaluation of the response to aluminum stress in olive tree (Olea europaea L.)
Authors: Niu Erli , Gao Song , Yu Xiaomin , Soleimani Ali , Zhu Shenlong
Published: July 2022

Olive (Olea europaea L.) is an ancient tree species in the Mediterranean, but the lack of knowledge about aluminum-resistant varieties limits its introduction to acidic soil. The objective of this study was to have a comprehensive evaluation of the response to aluminum stress in olive tree at germplasm, metabolome, and transcriptome levels. In this experiment, seedlings of 97 olive germplasm with 1.0–3.0 cm roots and two leaves were treated with 50 μM Al3+ (pH = 5.0). By factor analysis of the traits of defoliation rate, rooting rate, length of extended root, and length of new root, 97 germplasm were classified into five different groups according to their diverse responses to aluminum stress: 5 highly resistant (5.15%), 30 moderately resistant (30.93%), 31 general (31.96%), 23 moderately sensitive (23.71%), and 8 highly sensitive (8.25%) germplasm. The three most sensitive and three most resistant germplasm were further used for metabolome and transcriptome analysis. Exposed to aluminum stress, 96 differentially accumulated metabolites (DAMs)/4,845 differentially expressed genes (DEGs) and 66 DAMs/2,752 DEGs were identified in highly sensitive and resistant germplasm, respectively. Using multi-omics technology, the pathways and related DAMs/DEGs involved in cell wall/cytoplasm receptors, reactive oxygen species balance, hormone induction, synthesis of organic acids, Al3+ transport, and synthesis of metabolites were identified to mainly regulate the response to aluminum stress in olive. This study provides a theoretical guide and prior germplasm and genes for further genetic improvement of aluminum tolerance in the olive tree.
The role of legislation in K-12 school discipline: The silence of action
Authors: Bo Mengmeng , Onwubuya Gift Chinemerem
Published: July 2022

Researchers have consistently identified the disparity between teachers’ practical and legal knowledge regarding teachers’ right to discipline students. However, few studies have investigated teachers’ construction processes that form construction outcomes, which would help navigate the role of legislation in school discipline. This study contributes to a holistic picture of the neglected disciplinary rights that teachers construct in teaching practice and their underexplored attitude toward the law, using an interview-based constructionist method on twelve teachers of Lvliang city in a Chinese K-12 context. The findings suggest that the participants prefer to acquire discipline knowledge by interacting with multiple power relations in their local environment and that their knowledge is historically and culturally specific. Although the disciplinary right they construct is never static, balanced, or essentialised, the participants’ constructions are commonly not in line with legal provisions or the aims of the law. Furthermore, school discipline legislation plays a silent role in empowering teachers to discipline students, but it is more visible in holding back teachers’ use of corporal punishment. These findings illustrate the complexity of implementing school disciplinary law as a universal national policy.
Impact of absent crowds on technical and physical performances in the Chinese Soccer Super League
Authors: Chen Junjin , Zhai Shuaishuai , Xi Zenghui , Li Peilun , Zhang Shuolin
Published: July 2022

Purpose

Spectators have a significant impact on match performances in soccer, but to what extent crowd support contributes to the technical and physical performances remains unclear. This study aimed to (1) investigate the differences in terms of technical and physical performances with and without spectators; and (2) identify the key factors differentiating between win and loss when playing with and without the presence of an audience.

Methods

Our study examined 794 performance records from 397 matches during the 2019–2020 seasons in the Chinese Soccer Super League. The least absolute shrinkage and selection operator (LASSO)-logistic regression was utilized to select significant predictors. Using an independent t-test and the Mann–Whitney non-parametric test explores the difference between matches with and without spectators. Key factors between win and loss were explored using univariate and multivariate logistic regression analyses.

Results

Our study found that cross (p < 0.01, ES = −0.24), shots (p < 0.001, ES = −0.25), and shot accuracy (p < 0.05, ES = −0.18) displayed decreasing trends whereas sprint distances (p < 0.05, ES = 0.16) presented an increasing trend without spectators comparing with the crowd support. Moreover, the above three technical variables were the main factors differentiating between wins and losses. Similarly, team and opponent quality remained important potential factors affecting the match outcome.

Conclusion

Match outcome or team performance is determined by a myriad of factors, but there are clear differences in technical and physical performances between matches with and without the presence of an audience. Similarly, our study provides a better explanation for the impact of crowd support on match performances whereby coaches can deploy players and adjust match strategies for ultimate success.
Spatiotemporal variations of public opinion on social distancing in the Netherlands: Comparison of Twitter and longitudinal survey data
Authors: Zhang Chao , Wang Shihan , Tjong Kim Sang Erik , Adriaanse Marieke A. , Tummers Lars , Schraagen Marijn , Qi Ji , Dastani Mehdi , Aarts Henk
Published: July 2022

Background

Social distancing has been implemented by many countries to curb the COVID-19 pandemic. Understanding public support for this policy calls for effective and efficient methods of monitoring public opinion on social distancing. Twitter analysis has been suggested as a cheaper and faster-responding alternative to traditional survey methods. The current empirical evidence is mixed in terms of the correspondence between the two methods.

Objective

We aim to compare the two methods in the context of monitoring the Dutch public's opinion on social distancing. For this comparison, we quantified the temporal and spatial variations in public opinion and their sensitivities to critical events using data from both Dutch Twitter users and respondents from a longitudinal survey.

Methods

A longitudinal survey on a representative Dutch sample (n= 1,200) was conducted between July and November 2020 to measure opinions on social distancing weekly. From the same period, near 100,000 Dutch tweets were categorized as supporting or rejecting social distancing based on a model trained with annotated data. Average stances for the 12 Dutch provinces and over the 20 weeks were computed from the two data sources and were compared through visualizations and statistical analyses.

Results

Both data sources suggested strong support for social distancing, but public opinion was much more varied among tweets than survey responses. Both data sources showed an increase in public support for social distancing over time, and a strong temporal correspondence between them was found for most of the provinces. In addition, the survey but not Twitter data revealed structured differences among the 12 provinces, while the two data sources did not correspond much spatially. Finally, stances estimated from tweets were more sensitive to critical events happened during the study period.

Conclusions

Our findings indicate consistencies between Twitter data analysis and survey methods in describing the overall stance on social distancing and temporal trends. The lack of spatial correspondence may imply limitations in the data collections and calls for surveys with larger regional samples. For public health management, Twitter analysis can be used to complement survey methods, especially for capturing public's reactivities to critical events amid the current pandemic.
VOC Detections by p-Type Semiconducting Sensors Using Nano-Sized SmFeO3 Particles
Authors: Mori Masami , Noguchi Ayumu , Itagaki Yoshiteru
Published: July 2022

Nano-sized SmFeO3 particles are prepared by the pyrolysis of heteronuclear cyano-complex, Sm[Fe(CN)6]·4H2O at a temperature of 600 °C in ozone. The low temperature decomposition followed in ozone successfully yielded fine particles with a high specific surface area of 20.0 m3/g (sample A). The fine particles were classified into further smaller particles with a unimodal size distribution and this process yielded a high specific surface area of 26.0 m3/g (sample B). These semiconducting powders were deposited on a sensor electrode by electrophoretic deposition (EPD) and tested on their sensing properties to VOCs. The sensors consisting of samples A and B both showed good responses to ethanol at 285 and 320 °C. The sensor with sample B showed extraordinarily good selectivity of ethanol for toluene at 320 °C. This could be because the detection film of sample B with moderately grown particles selectively reduced the reaction activity of toluene. The sensor with sample B also exhibited good selectivity of ethanol for hexane and dichloromethane.
Teatro infantil. Una estrategia pedagógica de formación en la primera infancia
Authors: Salazar Arango Edwar , Rincón Gómez Sandra Lorena
Published: July 2022

El teatro infantil como estrategia de formación de la primera infancia, parte desde la intención de aportar alternativas de índole pedagógica, en correspondencia al currículo educativo del Ecuador; hacia una metodología del aprender haciendo. La investigación fue de tipo cualitativa, diseño descriptivo; los instrumentos de observación participante y entrevista con su respectivo procesamiento de información proporcionan desde el análisis e interpretación de datos, describir, explicar las experiencias de aprendizaje en niños de 4 a 5 años de edad, de la escuela “Juan Jacobo Rousseau”; en relación al teatro en su proceso formativo como fenómeno estudiado; en la adquisición y desarrollo de destrezas. A modo de conclusión; se puede describir de manera positiva la influencia del teatro infantil en el desarrollo cognitivo, procedimental y actitudinal; sirviendo esto, como base para potenciar ventajas de formación en comunicación, socialización en los diferentes contextos educativos desde el juego dramático; aportando al desarrollo integral infantil.
Reinserción social y reeducación del estudiante en centros penitenciarios
Authors: Ramos Huamán Wilmer Víctor
Published: July 2022

La reinserción social y reeducación de los jóvenes en los penales es parcialmente ignorado por el público general, ya que no se brinda información adecuada, ni conocen el gran trabajo que realizan los profesionales por brindarles un apoyo. El objetivo fue dar a conocer el tema de reinserción social y reeducación del estudiante en el centro penitenciario. La metodología según su fin, fue aplicada, de información cualitativa y con alcance de carácter correlacional, con una muestra de 121 internos. Como resultado de las clases brindado se pudo observar la existencia de un cambio de conducta, cognición y sensibilidad, este cambio de conciencia social se produjo con la ayuda de los familiares, concluyendo que este artículo busco mostrar en qué consisten los talleres y tratamientos de reinserción y reeducación, proponiendo un programa alternativo que se convierta en un proyecto para los penales, y así modificar todas aquellas conductas incorrectas o delictivas que los han llevado a la prisión.
Herramientas virtuales para mejorar las competencias digitales en los docentes en tiempos de pandemia
Authors: Díaz Ortiz Walter Demetrio , Mendocilla Martínez de Díaz Elena del Rosario , Merino Salazar Teresita del Rosario
Published: July 2022

La llegada de la pandemia generó que muchos docentes se replantearan sus procesos de enseñanza, sobre todo, en herramientas virtuales, por lo que el objetivo del presente estudio fue determinar si un programa de capacitación en herramientas virtuales permite mejorar las competencias digitales en docentes. La metodología fue de tipo aplicada. Su diseño fue pre-experimental. Se empleó la técnica de la encuesta y por instrumentos se usaron dos cuestionarios los cuales midieron las dos variables de estudio. La población fue de 77 docentes de los tres niveles de educación. Los datos recolectados fueron procesados y ordenados a darle respuesta a los objetivos del estudio y a las pruebas de hipótesis. Por resultados se obtuvo que existe una significativa mejora en el desempeño pedagógico de los docentes al emplear las plataformas tecnológicas, asimismo, en la información acerca de las bondades que ofrecen las nuevas tecnologías en contextos de pandemia.
Eficiencia en la resolución de tareas colaborativas presenciales y virtuales en universitarios
Authors: Mercado Contreras Cinthia Tomasa , Acuña Meléndrez Karla Fabiola , Rangel Bernal Nora Edith , Irigoyen Morales Juan José
Published: July 2022

La eficiencia del trabajo colaborativo es importante para asegurar el aprovechamiento de recursos usados en el logro de los objetivos en instituciones educativas y del sector privado, mediante interacciones presenciales y virtuales. Se realizó un estudio experimental con el objetivo de comparar la eficiencia en el trabajo colaborativo en díadas de estudiantes universitarios ante dos tareas realizadas presencial y virtualmente, con criterios abiertos y cerrados. Participaron 40 estudiantes de la Universidad de Sonora, desconocidos entre sí, con edades de entre17 a 31 años. Los resultados mostraron diferencias significativas en la cantidad de intervenciones entre los tipos de tarea y el tiempo de ejecución, pero no parecen tener una relación con el desempeño eficaz. Sin embargo, se evidenció un mayor desempeño de la tarea con criterio cerrado dentro del grupo con mayor interacción virtual y una mayor eficiencia en una tarea con criterio abierto dentro del grupo con mayor interacción presencial.
Niveles de autoconcepto, procesamiento estratégico de información y rendimiento académico en una universidad pública
Authors: Charry Aysanoa Juan , Rodas Serrano Virgilio Wilfredo , Yong Castañeda Cristian Alberto , Durand Saavedra Demetrio Pedro , Charry Aysanoa Pedro Amílcar
Published: July 2022

El autoconcepto y la capacidad de procesamiento de la información, son factores esenciales en la formación académica de estudiantes universitarios. El artículo demuestra la relación entre los niveles de autoconcepto y procesamiento estratégico de la información, con el rendimiento académico de los estudiantes de la carrera profesional de Ingeniería Agroindustrial de la Universidad Nacional Amazónica de Madre de Dios. El estudio desarrollado corresponde al nivel descriptivo asumiendo el diseño no experimental correlacional, en una muestra de 90 alumnos, donde se aplicó la técnica de la encuesta con dos instrumentos de recolección de datos: cuestionarios válidos y confiables. Como resultados de tiene que existe una relación inversa entre el autoconcepto y el rendimiento académico (r=-0.658) y el procesamiento estratégico de la información con el rendimiento (r=-0.464), por lo que se infiere que los estudiantes con niveles regulares de autoconcepto y capacidad de procesamiento de información, no presentan un buen rendimiento académico, lo que explica que existe una relación directamente inversa entre el autoconcepto y el procesamiento de la información con el rendimiento académico.
TKHK DEĞİŞİK m. 48 HÜKMÜ ÇERÇEVESİNDE ARACI HİZMET SAĞLAYICININ TÜKETİCİYE KARŞI SORUMLULUĞU
Authors: KESKİN Dilşad
Published: July 2022

Mesafeli sözleşmelere ilişkin TKHK m. 48 hükmü, 7392 sayılı Kanunun 7. maddesiyle, yayımı tarihinden altı ay sonra (1/10/2022) yürürlüğe girmek üzere değiştirilmiştir. Yeni düzenlemede “aracılık edenler” ifadesi yerine “aracı hizmet sağlayıcılar” terimi kullanılmış ve aracı hizmet sağlayıcıların tüketiciye yönelik yükümlülüklerinin kapsamı genişletilmiştir. Hükmün önceki versiyonunda aracılık edenlerin satıcı/sağlayıcı ile yapılan işlemlere ilişkin kayıt tutarak talep halinde bunları paylaşmakla yükümlü oldukları ve satıcı/sağlayıcı ile yaptıkları sözleşmeye aykırı fiillerinden sorumlu olacakları ifade edilmişti. TKHK değişik m. 48 hükmü ise bu hususlara ek olarak aracı hizmet sağlayıcıların, tüketicinin talep ve bildirimlerini iletip takip edebileceği bir sistemin kurulması ve açık tutulması ile tüketiciye ön bilgilendirme yapılması ve ön bilgilendirmenin teyidi ve ispatından sorumlu olacağını düzenlemektedir. Aracı hizmet sağlayıcı, aracılık sözleşmesine aykırı uygulamaları nedeniyle satıcı/sağlayıcıların bu madde hükümlerine aykırı davranmasına sebep oldukları işlemlerden, satıcı/sağlayıcı adına bedel tahsil etmesi hâlinde teslim veya ifa ile cayma hakkına ilişkin yükümlülüklerden, satıcı/sağlayıcı onayı olmaksızın düzenlediği kampanyalı, promosyonlu veya indirimli satışlarda sözleşmenin hiç ya da gereği gibi ifa edilmemesinden de sorumludur.
RENDIMIENTO DE LA CALABAZA PIPIANA EN RESPUESTA A LA PODA Y LA DENSIDAD DE POBLACIÓN
Authors: Ayvar-Serna Sergio , Mena-Bahena Antonio , Cortés-Moreno Doribel , Durán-Ramírez José Aurelio , De Luna-Moreno José Guillermo
Published: July 2022

Con el objetivo de evaluar el comportamiento agronómico de la calabaza pipiana (Cucurbita argyrosperma Huber) en el rendimiento de semilla, se hizo esta investigación en Cocula, Gro. Se evaluaron siete distancias entre matas (30, 60, 90, 120, 150, 180 y 210 cm), en combinación con dos niveles de poda (sin y con poda); los 14 tratamientos se establecieron en campo bajo un diseño experimental de bloques completos al azar en arreglo de parcelas divididas, con cuatro repeticiones. Los tratamientos estudiados mostraron diferencias significativas en cuanto al tamaño de la planta, el número de frutos y al peso de semilla. Las densidades de 26 666 y 8 888 plantas/ha (30 y 90 cm entre matas) produjeron la mayor cantidad de frutos.
An Implementation of Traffic Signs and Road Objects Detection Using Faster R-CNN
Authors: Güney Emin , Bayılmış Cüneyt
Published: July 2022

Traffic signs and road objects detection is significant issue for driver safety. It has become popular with the development of autonomous vehicles and driver-assistant systems. This study presents a real-time system that detects traffic signs and various objects in the driving environment with a camera. Faster R-CNN architecture was used as a detection method in this study. This architecture is a well-known two-stage approach for object detection. Dataset was created by collecting various images for training and testing of the model. The dataset consists of 1880 images containing traffic signs and objects collected from Turkey with the GTSRB dataset. These images were combined and divided into the training set and testing set with the ratio of 80/20. The model's training was carried out in the computer environment for 8.5 hours and approximately 10000 iterations. Experimental results show the real-time performance of Faster R-CNN for robustly traffic signs and objects detection.