bluuebunny/crossref_metadata_2025 huggingface dataset:
The Interplay of Growth Mechanism and Properties of ZnO Nanostructures for Different Applications
Authors: Clarke Breanna , Ghandi Khashayar
Published: July 2023

This review provides a background on the structure and properties of ZnO nanostructures. ZnO nanostructures are advantageous for many applications in sensing, photocatalysis, functional textiles, and cosmetic industries, which are described in this review. Previous work using UV Visible (UV–vis) spectroscopy and scanning electron microscopy (SEM) for ZnO nanorod growth analysis in‐solution and on a substrate for determination of optical properties and morphology is discussed, as well as their results in determining the kinetics and growth mechanisms. From this literature review, it is understood that the synthesis process greatly affects nanostructures and properties; and hence, their applications. In addition, in this review, the mechanism of ZnO nanostructure growth is unveiled, and it is shown that by having greater control over their morphology and size through such mechanistic understanding, the above‐mentioned applications can be affected. The contradictions and gaps in knowledge are summarized in order to highlight the variations in results, followed by suggestions for how to answer these gaps and future outlooks for ZnO nanostructure research.
Combined x‐ray reflectivity and grazing incidence x‐ray fluorescence study of Ta/Cr/Pt thin film stacks
Authors: Jonnard Philippe , Le Guen Karine , Delaunay Renaud , Ménesguen Yves , Lépy Marie‐Christine , Briand Emrick , Schmaus Didier , Vickridge Ian
Published: July 2023

The Ta/Cr/Pt three‐layer system can be used as a planar x‐ray waveguide, that is to say it can guide an x‐ray beam inside its chromium layer. This property comes from the difference in density and hence in optical index between the two “heavy” or cladding tantalum and platinum layers and the “light” or guiding chromium layer. The waveguide will be efficient provided the layers are a few nanometers thick and that the interfaces are as sharp as possible. To control the quality of the stack, we combine grazing incidence x‐ray fluorescence (GIXRF) and x‐ray reflectivity (XRR) measurements on a series of Ta/Cr/Pt samples, whose only difference is the thickness of the Cr layer. The three considered samples have been deposited by magnetron sputtering and their designed structures are: Ta (8 nm)/Cr (5, 10, and 15 nm)/Pt (14 nm)/Si substrate. The combination of XRR and GIXRF tightens constraints on the parameters used to simulate the stack: thickness, roughness, composition, and density of the layers and their interlayers. For each sample we used six GIXRF curves obtained from three different characteristic x‐ray lines (Ta Lα, Cr Kα, and Pt Lβ2,15) excited at three different incident photons energies (6.25, 10, and 12 keV) as well as one XRR curve obtained at 6.25 keV. The XRR‐GIXRF combined analysis demonstrates that the Ta/Cr/Pt structure is too simplistic and that it is necessary to introduce some interlayers at the top and bottom of the stacks to obtain a reliable agreement between the experimental and simulated GIXRF and XRR curves.
Can surgically assisted rapid palatal expansion (SARPE) be recommended over orthodontic rapid palatal expansion (ORPE) for girls above the age of 14?
Authors: Govaerts Dries , Da Costa Oliver , Garip Melisa , Combes François , Jacobs Reinhilde , Politis Constantinus
Published: July 2023

Background

For patients with a maxillary transversal deficiency (MTD), various treatment options are available, partly based on the practitioner’s experience. This study aimed to determine a cut-off age for decision making between surgically assisted rapid palatal expansion (SARPE) over orthodontic rapid palatal expansion (ORPE) based on skeletal maturation in a female population.

Methods

A total of 100 cone beam computed tomography (CBCT) images of young females were analyzed on maturation of the pterygomaxillary (PMS), zygomaticomaxillary (ZMS), transpalatal (TPS), and midpalatal (MPS) sutures. Based on the maturation of these four junctions, four independent observers had to determine whether they would prefer ORPE or SARPE to widen the maxilla.

Results

For the PMS, the results show a closure of 83–100% from 13 to 17 years. As for the TPS, a closure of 78–85% was observed from 15 years of age. For the 15- to 17-year-old females, a closed ZMS was present in 32–47%. Regarding MPS, closed sutures presented in 61% (stages D and E) of the 15-year-old females. The cut-off age at which SARPE was recommended was 15.1 years for the orthodontist observers and 14.8 years for the maxillofacial surgeon observers.

Conclusions

Significant maturation of MPS was reached at the age of 15 in a female population. The PMS, TPS, MPS, and ZMS closed sequentially. A comprehensive diagnostic approach is necessary for choosing the appropriate treatment. When in doubt, age could assist decision making in a female population, with a cut-off age of 15 years in favor of SARPE based on this study.
The dynamics of labor force participation: Is all quiet on the Appalachian front?
Authors: Beverly Josh , Stewart Shamar L. , Neill Clinton L.
Published: July 2023

This study examines the divergence and synchronicity of labor force participation rate (LFPR) dynamics across the USA. Using a dynamic factor model with time-varying stochastic volatility, we decompose each state’s LFPR into a national, regional, and state-specific latent factor. We find significant time variation in our factors and heterogeneous labor market responses and relative sensitivities. Our results show that, save for West Virginia, there is no strong Appalachian regional component, and instead, the national and state-specific components explain much of the variation in state LFPRs. Our results suggest the need for more targeted and localized labor market policies during periods of divergence in LFPRs (i.e., recessions and shocks) and federal policies during national economic booms or periods of recovery.
Suppression of sonic hedgehog pathway-based proliferation in glioblastoma cells by small-size silver nanoparticles in vitro
Authors: Skóra Bartosz , Masicz Martyna , Nowak Patrycja , Lachowska Jagoda , Sołtysek Paulina , Biskup Justyna , Matuszewska Paulina , Szychowski Konrad A.
Published: July 2023

Glioblastomas (GBs) are one of the most aggressive and invasive intracranial cancers. Recently, it has been postulated that, among other factors, the hedgehog (HH) pathway may be a key factor in this phenomenon. Moreover, it has been reported that small-size silver nanoparticles (AgNPs) are characterized by a high cytotoxic effect towards GBs. However, their effect on the sonic hedgehog (SHH) pathway has never been demonstrated in any cancer cells. Therefore, the aim of the present study was to evaluate the impact of the anti-proliferative properties of 5-nm AgNPs on the SHH pathway in the GB cell line (U-87MG) in vitro. The results showed a time- and dose-dependent decrease in the metabolic activity in the U-87MG cells treated with AgNPs, with IC50 reaching 30.41 and 21.16 µg/mL after 24 h and 48 h, respectively, followed by an increase in the intracellular reactive oxygen species (ROS) level. The co-treatment of the cells with AgNPs and Robotnikinin (SHH inhibitor) abolished and/or strengthened the effect of AgNPs, especially on the SHH mRNA levels and on the PCNA, PTCH1, Gli1, and SUFU protein levels. Interestingly, no changes in the level of ERK1/2, Akt, and SRC kinase protein expression were detected, suggesting a direct impact of AgNPs and/or ROS on the inhibition of the canonical SHH pathway. However, more studies are needed due to the increase in the mTOR protein expression after the treatment of the cells with AgNPs, as in the Robotnikinin treatment. In conclusion, small-size AgNPs are able to inhibit the proliferation of GB cells in vitro by suppressing the canonical SHH pathway.
Correlation Energy of a Weakly Interacting Fermi Gas with Large Interaction Potential
Authors: Benedikter Niels , Porta Marcello , Schlein Benjamin , Seiringer Robert
Published: July 2023

Recently the leading order of the correlation energy of a Fermi gas in a coupled mean-field and semiclassical scaling regime has been derived, under the assumption of an interaction potential with a small norm and with compact support in Fourier space. We generalize this result to large interaction potentials, requiring only$$|\cdot | \hat{V} \in \ell ^1 (\mathbb {Z}^3)$$|·|V^∈ℓ1(Z3). Our proof is based on approximate, collective bosonization in three dimensions. Significant improvements compared to recent work include stronger bounds on non-bosonizable terms and more efficient control on the bosonization of the kinetic energy.
Effects of the dopamine depleting agent tetrabenazine in tests evaluating different components of depressive-like behavior in mice: sex-dependent response to antidepressant drugs with SERT and DAT blocker profiles
Authors: Carratalá-Ros Carla , Martínez-Verdú Andrea , Olivares-García Regulo , Salamone John D. , Correa Mercè
Published: July 2023

Background

Depression is a disorder twice as common in women than in men. There are sex differences in the symptomatology and treatment response to this disorder. Impairments in behavioral activation (i.e. anergia, fatigue) are often seen in people with depression and are highly resistant to treatment. The role of mesolimbic dopamine (DA) in regulating behavioral activation has been extensively studied in male rodents, but little is known in female rodents.

Objective

The present studies assessed potential sex differences in rodent paradigms used to study different components of depressive-like behavior, and in the treatment response to antidepressants with different mechanisms of action.

Methods

Male and female CD1 mice received Tetrabenazine (TBZ), a VMAT-2 blocker that depletes DA and induces depressive symptoms in humans. Mice were tested on the Forced Swim Test, (FST), the Dark–Light box (DL), the elevated plus maze (EPM), Social Interaction (SI) test, and sucrose preference and consumption using the two bottles test. In addition, bupropion (a DA reuptake inhibitor) or fluoxetine (a serotonin reuptake inhibitor) were used to reverse TBZ-induced anergia.

Results

In the FST, bupropion reversed TBZ effects in both sexes but fluoxetine was only effective in female mice. DA depletion did not affect other aspects of depression such as anxiety, sociability or sucrose consumption, and there was no interaction with bupropion on these parameters. In TBZ treated-females SERT-blockers may be effective at reversing anergia in aversive contexts (FST), and potentiating avoidance of anxiogenic stimuli.

Conclusions

Pro-dopaminergic antidepressants seem more efficacious at improving anergia in both sexes than SERT-blockers.
Stomach content analysis of North Sea cephalopods: often-overlooked predators with direct impact on commercially used fish species?
Authors: Oesterwind Daniel , Piatkowski Uwe
Published: July 2023

The study of marine food web models has increased during the last years, but input data of important groups such as cephalopods are missing sometimes which restricts the quality of the model results. Cephalopods feed on a variety of preys, ranging from small crustaceans to large commercially important fish species. In turn, they are taken by larger invertebrates, fish, cephalopods, marine mammals and seabirds, which emphasizes their important role in various marine food webs. Our study presents stomach content analyses of various cephalopod species from the North Sea and describes their general feeding trends. The results further support the inclusion of cephalopods as predators into food web models to increase our knowledge of the North Sea ecosystem and to improve its management. Our data in combination with observed increasing biomasses of North Sea cephalopods suggest that the impact of cephalopods in the North Sea food web has increased and that large-sized cephalopods have become more important as predators for commercially exploited fish species during recent years.
Added value of zoomed-echo-planar imaging diffusion-weighted imaging for evaluation of periampullary carcinomas
Authors: Liu Jingjing , Huang Mengyue , Ren Yanan , Xu Man , Zhu Jinxia , Li Yinhua , Cheng Jingliang
Published: July 2023

Background

To assess the image quality feasibility and diagnostic value of zoomed diffusion-weighted imaging (z-EPI DWI) using echo-planar imaging (EPI) compared with conventional DWI (c-EPI DWI) in patients with periampullary disease.

Methods

Thirty-six patients with periampullary carcinomas and fifteen with benign periampullary disease were included in this study. All the subjects underwent MR cholangiopancreatography (MRCP), c-EPI DWI, and z-EPI DWI. Two radiologists independently assessed image quality of the two image sets, including overall image quality and lesion conspicuity. In addition, signal intensity and ADC measurements of DWIs in the periampullary lesions were conducted. Diagnostic accuracies of the combined image sets of MRCP and z-EPI DWI were compared with those of a combined set of MRCP and c-EPI DWI.

Results

z-EPI DWI showed significantly better image quality scores (anatomic structure visualization, 2.94 ± 0.24; overall image quality, 2.96 ± 0.17) compared to that with c-EPI DWI (anatomic structure visualization, 2.02 ± 0.22; overall image quality, 2.04 ± 0.24) (both P < 0.01). For all the periampullary malignant lesions and small lesions (≤ 20 mm), there was better delineation of lesion conspicuity and the lesion margin, as well as diagnostic confidence with z-EPI DWI (all P < 0.05). The rate of periampullary malignancy’s hyperintense signal on z-EPI DWI was increased to 91.7% (33/36) compared to c-EPI DWI (69.4% (25/36)) (P = 0.023). For all malignant lesions and small lesions, the diagnostic accuracy scores were increased using the MRCP and z-EPI DWI combined set, compared to the MRCP and c-EPI DWI combined set (P < 0.05). Diagnostic accuracy for detection and differentiation of malignant lesions from benign lesions significantly improved for the MRCP and z-EPI DWI combined set compared with MRCP and c-EPI DWI combined set (P < 0.05). There were no significant differences between c-EPI DWI and z-EPI DWI in the ADC values of periampullary malignant and benign lesions (P > 0.05).

Conclusions

z-EPI DWI has an advantage that could lead to remarkable image quality improvements and enhanced lesion visualization of periampullary carcinomas. z-EPI DWI was superior to c-EPI DWI for detecting, delineating, and diagnosing the lesions, particularly for small challenging lesions.

Graphical abstract
The current evidence on surgical management for synchronous bilateral renal tumors: results from a scoping review
Authors: Giulioni Carlo , Maggi Martina , Pirola Giacomo Maria , Martorana Eugenio , Cormio Angelo , Teoh Jeremy Yuen-Chun , Gauhar Vineet , Galosi Andrea Benedetto , Castellani Daniele
Published: July 2023

Purpose

Synchronous bilateral renal masses (SBRM) account for a low percentage of kidney tumors, and there is no current recommendation for their management. The objective was to review evidence regarding the best surgical approach for SBRM in terms of type and timing of surgery.

Methods

A broad literature search was performed on 28th January 2023 using Scopus, PubMed, and EMBASE. Only English papers dealing with adults were included. Meeting abstracts were excluded.

Results

Twenty-four papers were accepted and included. SBRM behave less aggressively than metachronous tumors, and partial nephrectomy (PN) is the preferred therapeutic option to preserve renal function. Open, laparoscopic, and robot-assisted approaches were found to be similar in oncological outcomes, though robot-assisted surgery resulted in lower comorbidities. Same-sitting PN was demonstrated to be a safe approach, particularly in the robotic-assisted one. Finally, the same-siting and staged NSS were similar in preserving renal function.

Conclusions

PN should be the desirable treatment for SBRM whenever feasible and if patients are fit, but surgeon expertise should also be taken into the account.
Convection-permitting climate simulations for South America with the Met Office Unified Model
Authors: Halladay Kate , Kahana Ron , Johnson Ben , Still Christopher , Fosser Giorgia , Alves Lincoln
Published: July 2023

We present the first convection-permitting regional climate model (CPRCM) simulations at 4.5 km horizontal resolution for South America at near-continental scale, including full details of the experimental setup and results from the reanalysis-driven hindcast and climate model-driven present-day simulations. We use a range of satellite and ground-based observations to evaluate the CPRCM simulations covering the period 1998–2007 comparing the CPRCM output with lower resolution regional and global climate model configurations for key regions of Brazil. We find that using the convection-permitting model at high resolution leads to large improvements in the representation of precipitation, specifically in simulating its diurnal cycle, frequency, and sub-daily intensity distribution (i.e. the proportion of heavy and light precipitation). We tentatively conclude that there are also improvements in the spatial structure of precipitation. We see higher precipitation intensity and extremes over Amazonia in the CPRCMs compared with observations, though more sub-daily observational data from meteorological stations are required to conclusively determine whether the CPRCMs add value in this regard. For annual mean precipitation and mean, maximum and minimum near surface temperatures, it is not clear that the CPRCMs add value compared with coarser-resolution models with parameterised convection. We also find large changes in the contribution to evapotranspiration from canopy evaporation compared to soil evaporation and transpiration compared with the RCM. This is likely to be related to the shift in precipitation intensity distribution of the CPRCMs compared to the RCM and its impact on the hydrological requires further investigation.
Reliability of whole mount radical prostatectomy histopathology as the ground truth for artificial intelligence assisted prostate imaging
Authors: Jager Auke , Postema Arnoud W. , van der Linden Hans , Nooijen Peet T.G.A. , Bekers Elise , Kweldam Charlotte F. , Daures Gautier , Zwart Wim , Mischi M. , Beerlage Harrie P. , Oddens Jorg R.
Published: July 2023

The development of artificial intelligence–based imaging techniques for prostate cancer (PCa) detection and diagnosis requires a reliable ground truth, which is generally based on histopathology from radical prostatectomy specimens. This study proposes a comprehensive protocol for the annotation of prostatectomy pathology slides. To evaluate the reliability of the protocol, interobserver variability was assessed between five pathologists, who annotated ten radical prostatectomy specimens consisting of 74 whole mount pathology slides. Interobserver variability was assessed for both the localization and grading of PCa. The results indicate excellent overall agreement on the localization of PCa (Gleason pattern ≥ 3) and clinically significant PCa (Gleason pattern ≥ 4), with Dice similarity coefficients (DSC) of 0.91 and 0.88, respectively. On a per-slide level, agreement for primary and secondary Gleason pattern was almost perfect and substantial, with Fleiss Kappa of .819 (95% CI .659–.980) and .726 (95% CI .573–.878), respectively. Agreement on International Society of Urological Pathology Grade Group was evaluated for the index lesions and showed agreement in 70% of cases, with a mean DSC of 0.92 for all index lesions. These findings show that a standardized protocol for prostatectomy pathology annotation provides reliable data on PCa localization and grading, with relatively high levels of interobserver agreement. More complicated tissue characterization, such as the presence of cribriform growth and intraductal carcinoma, remains a source of interobserver variability and should be treated with care when used in ground truth datasets.
Renal neuroendocrine tumors: clinical and molecular pathology with an emphasis on frequent association with ectopic Cushing syndrome
Authors: Kasajima Atsuko , Pfarr Nicole , von Werder Alexander , Schwamborn Kristina , Gschwend Jürgen , Din Nasir Ud , Esposito Irene , Weichert Wilko , Pavel Marianne , Agaimy Abbas , Klöppel Günter
Published: July 2023

Renal neuroendocrine tumors (RenNETs) are rare malignancies with largely unknown biology, hormone expression, and genetic abnormalities. This study aims to improve our understanding of the RenNETs with emphasis of functional, hormonal, and genetic features. Surgically resected RenNETs (N = 13) were retrieved, and immunohistochemistry and next-generation sequencing (NGS) were performed in all cases. In addition, all published RenNETs were systematically reviewed. Our cohort (4 men and 9 women, mean age 42, mean tumor size 7.6 cm) included 2 patients with Cushing syndrome (CS). WHO grade (23% G1, 54% G2, and 23% G3) and tumor progression did not correlate. CS-associated RenNETs (CS-RenNETs) showed a solid and eosinophilic histology and stained for ACTH, while the remaining non-functioning tumors had a trabecular pattern and expressed variably hormones somatostatin (91%), pancreatic polypeptide (63%), glucagon (54%), and serotonin (18%). The transcription factors ISL1 and SATB2 were expressed in all non-functioning, but not in CS-RenNETs. NGS revealed no pathogenic alterations or gene fusions. In the literature review (N = 194), 15 (8%) of the patients had hormonal syndromes, in which CS being the most frequent (7/15). Large tumor size and presence of metastasis were associated with shorter patients’ survival (p < 0.01). RenNETs present as large tumors with metastases. CS-RenNETs differ through ACTH production and solid-eosinophilic histology from the non-functioning trabecular RenNETs that produce pancreas-related hormones and express ISL1 and SATB2. MEN1 or DAXX/ARTX abnormalities and fusion genes are not detected in RenNETs, indicating a distinct yet unknown molecular pathogenesis.
Does sequential examination under anaesthesia provide a reliable method to determine a management plan for unstable lateral compression pelvic ring injuries? a prospective study
Authors: Shawky Mostafa Ahmed , Abdelazeem Ahmed Hazem , Abdel-Kader Khaled Fawzy , Mohammad Molham Mahmood , Azzam Ahmad Hamdi
Published: July 2023

Purpose

To assess the reliability of sequential examination under anaesthesia (EUA) to determine pelvic instability and to evaluate radiological and functional outcomes in unstable lateral compression (LC) injuries.

Methods

A prospective case series study was conducted from 2020 to 2022 at a university hospital on 43 cases with LC injuries that met the inclusion criteria. Sequential EUA was carried out in three steps. Posterior-only fixation or anterior–posterior fixation was done according to the algorithm. Each patient was followed up for at least 12 months, both radiologically and functionally.

Results

Forty cases proved unstable and were fixed. None showed secondary displacement in the anterior–posterior fixation group. However, five cases (19.2%) of the posterior-only fixation group showed secondary displacement with a mean of 5.9 mm. Four cases of them had tetra-ramic injuries. There is a high tendency for secondary displacement at 14.5 mm or more preoperative displacement of the rami. Patients with secondary displacement showed comparable functional outcome scores to patients without secondary displacement. Posterior-only fixation showed shorter operative time, lesser radiological exposure, blood loss and iatrogenic nerve injury than anterior–posterior fixation.

Conclusion

EUA is a reliable method to determine pelvic instability and management plan for LC fractures with unilateral anterior ring injury. Anterior–posterior fixation is needed if there is a tetra-ramic fracture or initial anterior ring displacement of 14.5 mm or more, irrespective of EUA.
Perception-based high quality distributed virtual reality
Authors: Naylor William , Debattista Kurt , Chalmers Alan
Published: July 2023

Virtual reality has great potential to enable remote collaborative work from anywhere in the world. Developing virtual reality into a platform suitable for natural interaction and immersive collaboration requires the experience to be reliably stable. For a networked collaborative environment, perceived smoothness of motion is limited by the tick rate, that is, the frequency at which information is distributed. As tick rate increases, motion will appear increasingly smooth; however, excessive tick rates may introduce additional load on a network without any perceptible benefit to a user. This paper details two visual psychophysics experiments ($$N_1 = 16$$

N
1

=
16

, $$N_2 = 11$$

N
2

=
11

) carried out to evaluate participant sensitivity to tick rate in virtual reality. The influence of three variables, velocity, complexity, and digital medium were investigated. Both velocity and digital medium displayed a significant effect, whilst complexity did not show significance. A model was then built and validated from the results of these experiments. The model predicts for average walking speed within the desktop condition, that 90% of the population will perceive motion to be smooth at 56 Hz, whilst this 90% threshold lies at 113 Hz for the VR condition. This model can predict participant perception of tick rate under given conditions, enabling networks to intelligently optimise participant experience without adding unnecessary further load on the network.
Burden of disease and real-world treatment patterns of patients with systemic lupus erythematosus in the Australian OPAL dataset
Authors: Ciciriello Sabina , Littlejohn Geoffrey , O’Sullivan Catherine , Smith Tegan , Deakin Claire T.
Published: July 2023

Objective

To describe the demographics, disease burden and real-world management of patients with systemic lupus erythematosus (SLE) in Australian community practice.

Methods

Patients with a physician diagnosis of SLE and at least 1 visit between 1 January 2009 and 31 March 2021 were identified in the OPAL dataset, an aggregated collection of data extracted from the electronic medical records of patients managed by 112 Australian rheumatologists. Demographics, basic clinical features and prescribed medications were described, with medication combinations used as a surrogate of disease severity.

Results

Of 5133 patients with a diagnosis of lupus, 4260 (83%) had SLE. Of these SLE patients, almost 90% of patients were female, with a median age of 49 years [IQR 37–61] at first-recorded visit. Of the 2285 SLE patients whose most recent visit was between 1 January 2019 and 31 March 2021, 52.5% had mild disease, 29.9% had moderate-severe disease and 7.4% had very severe disease. Visit frequency increased with disease severity. Most patients (85.8%) were treated with hydroxychloroquine, typically prescribed as first line-of-therapy.

Conclusion

In this large real-world Australian cohort of patients with SLE, a substantial burden of disease was identified, with a significant proportion (almost one-third of patients) considered to have moderate to severe disease based on medication use. This study provides a greater understanding of the path from symptom onset to treatment and the heterogeneous presentation of patients with SLE who are treated in community practice in Australia.

Key messages• Most published studies describing patients with SLE are derived from specialist lupus centres, typically in the hospital setting, therefore little is known about the characteristics of patients with SLE who are receiving routine care in community clinics.• The OPAL dataset is a large collection of clinical data from the electronic medical records of rheumatologists predominantly practising in private community clinics, which is where the majority (73–80%) of adult rheumatology services are conducted in Australia [1–3] . Since data from community care has not been widely available for SLE research, this study contributes important insight into this large and under-reported patient population.• To improve access to care and effective treatments, and reduce the burden of SLE in Australia, a greater understanding of the characteristics and unmet needs of patients with SLE managed in the community setting is required.
A systematic review of ambulance service-based randomised controlled trials in stroke
Authors: Dixon Mark , Appleton Jason P. , Siriwardena A. Niroshan , Williams Julia , Bath Philip M.
Published: July 2023

Background

Treatment for stroke is time-dependent, and ambulance services play a vital role in the early recognition, assessment and transportation of stroke patients. Innovations which begin in ambulance services to expedite delivery of treatments for stroke are developing. However, research delivery in ambulance services is novel, developing and not fully understood.

Aims

To synthesise literature encompassing ambulance service-based randomised controlled interventions for acute stroke with consideration to the characteristics of the type of intervention, consent modality, time intervals and issues unique to research delivery in ambulance services.

Summary of review

Online searches of MEDLINE, EMBASE, Web of Science, CENTRAL and WHO IRCTP databases and hand searches identified 15 eligible studies from 538. Articles were heterogeneous in nature and meta-analysis was partially available as 13 studies reported key time intervals, but terminology varied. Randomised interventions were evident across all points of contact with ambulance services: identification of stroke during the call for help, higher dispatch priority assigned to stroke, on-scene assessment and clinical interventions, direct referral to comprehensive stroke centres and definitive care delivery at scene. Consent methods ranged between informed patient, waiver and proxy modalities with country-specific variation. Challenges unique to the prehospital setting comprise the geographical distribution of ambulance resources, low recruitment rates, prolonged recruitment phases, management of investigational medicinal product and incomplete datasets.

Conclusion

Research opportunities exist across all points of contact between stroke patients and ambulance services, but randomisation and consent remain novel. Early collaboration and engagement between trialists and ambulance services will alleviate some of the complexities reported.

Registration number

PROSPERO 2018CRD42018075803
A systematic review of the use of bacteriophages for in vitro biofilm control
Authors: Meneses Luciana , Brandão Ana Catarina , Coenye Tom , Braga Ana Cristina , Pires Diana Priscila , Azeredo Joana
Published: July 2023

Bacteriophages (phages) are very promising biological agents for the prevention and control of bacterial biofilms. However, little is known about the parameters that can influence the efficacy of phages on biofilms. This systematic review provides a summary and analysis of the published data about the use of phages to control pre-formed biofilms in vitro, suggesting recommendations for future experiments in this area. A total of 68 articles, containing data on 605 experiments addressing the efficacy of phages to control biofilms in vitro were included, after a search conducted in Web of Science, Embase, and Medline (PubMed). The data collected from each experiment included information about biofilm growth conditions, phage characteristics, treatment conditions and biofilm reduction. In most cases, biofilms were formed in the surface of microtiter plates (82.5%); the median time for biofilm formation was 24 h, as is the median treatment duration. Quantification of biofilm biomass (52.6%), viable cells (25.5%) and metabolic activity (17.9%) were the most common biofilm assessment methods. Correlation analysis revealed that some phage parameters can influence the treatment outcome: higher phage concentrations were strongly associated with improved biofilm control, leading to higher levels of biofilm reduction, and phages with higher burst sizes and shorter latent periods seem to be the best candidates to control biofilms in vitro. However, the great variability of the methodologies used prompts the need for the development of standardized in vitro methodologies to characterize phage/biofilm interactions and to assess the efficacy of phages to control biofilms.
Post-infarct evolution of ventricular and myocardial function
Authors: Janssens K. L. P. M. , Kraamer M. , Barbarotta L. , Bovendeerd P. H. M.
Published: July 2023

Adverse ventricular remodeling following acute myocardial infarction (MI) may induce ventricular dilation, fibrosis, and loss of global contractile function, possibly resulting in heart failure (HF). Understanding the relation between the time-dependent changes in material properties of the myocardium and the contractile function of the heart may further our understanding of the development of HF post-MI and guide the development of novel therapies. A finite element model of cardiac mechanics was used to model MI in a thick-walled truncated ellipsoidal geometry. Infarct core and border zone comprised 9.6 and 8.1% of the LV wall volume, respectively. Acute MI was modeled by inhibiting active stress generation. Chronic MI was modeled by the additional effect of infarct material stiffening, wall thinning and fiber reorientation. In acute MI, stroke work decreased by 25%. In the infarct core, fiber stress was reduced but fiber strain was increased, depending on the degree of infarct stiffening. Fiber work density was equal to zero. Healthy tissue adjacent to the infarct showed decreased work density depending on the degree of infarct stiffness and the orientation of the myofibers with respect to the infarct region. Thinning of the wall partially restored this loss in work density while the effects of fiber reorientation were minimal. We found that the relative loss in pump function in the infarcted heart exceeds the relative loss in healthy myocardial tissue due to impaired mechanical function in healthy tissue adjacent to the infarct. Infarct stiffening, wall thinning and fiber reorientation did not affect pump function but did affect the distribution of work density in tissue adjacent to the infarct.
Electrophysiological Correlates of Reward Anticipation in Subjects with Schizophrenia: An ERP Microstate Study
Authors: Perrottelli A. , Giordano G. M. , Koenig T. , Caporusso E. , Giuliani L. , Pezzella P. , Bucci P. , Mucci A. , Galderisi S.
Published: July 2023

The current study aimed to investigate alterations of event-related potentials (ERPs) microstate during reward anticipation in subjects with schizophrenia (SCZ), and their association with hedonic experience and negative symptoms. EEG data were recorded in thirty SCZ and twenty-three healthy controls (HC) during the monetary incentive delay task in which reward, loss and neutral cues were presented. Microstate analysis and standardized low-resolution electromagnetic tomography (sLORETA) were applied to EEG data. Furthermore, analyses correlating a topographic index (the ERPs score), calculated to quantify brain activation in relationship to the microstate maps, and scales assessing hedonic experience and negative symptoms were performed. Alterations in the first (125.0–187.5 ms) and second (261.7–414.1 ms) anticipatory cue-related microstate classes were observed. In SCZ, reward cues were associated to shorter duration and earlier offset of the first microstate class as compared to the neutral condition. In the second microstate class, the area under the curve was smaller for both reward and loss anticipation cues in SCZ as compared to HC. Furthermore, significant correlations between ERPs scores and the anticipation of pleasure scores were detected, while no significant association was found with negative symptoms. sLORETA analysis showed that hypo-activation of the cingulate cortex, insula, orbitofrontal and parietal cortex was detected in SCZ as compared to HC. Abnormalities in ERPs could be traced already during the early stages of reward processing and were associated with the anticipation of pleasure, suggesting that these dysfunctions might impair effective evaluation of incoming pleasant experiences. Negative symptoms and anhedonia are partially independent results.
Size-Dependent Enforcement, Tax Evasion and Dimensional Trap
Authors: Coppier Raffaella , Michetti Elisabetta , Scaccia Luisa
Published: July 2023

Size-dependent tax enforcement is quite widespread worldwide, but the literature on its effects over firms’ behaviour is very scarce. By assuming different audit probabilities for small and large firms, we propose a dynamic model to study the consequences of size-dependent monitoring level on a firm’ fiscal compliance and its decision to invest and grow in a single-firm perspective. By combining analytical findings and simulation results, we show that: (1) under certain conditions, a dimensional trap may emerge, as small firms have no advantage in growing and prefer to remain small to avoid stronger enforcement; (2) audit activity and fine levels are important tools available to the State to fight evasion, but a careful calibration is required not to incur undesired effects.
TV Consumption Patterns and the Impact of Media Freedom on Political Trust and Satisfaction with the Government
Authors: Knoll Bodo , Pitlik Hans , Rode Martin
Published: July 2023

This paper empirically assesses the impact of media freedom on citizen’s trust in politicians and satisfaction with national governments. Restrictions potentially allow governments to provide citizens with biased information, which may then increase trust in, and satisfaction with, ruling elites. Yet, these restrictions may also be perceived as a signal that the latter are not trustworthy. Employing data from the European Social Survey to compare respondents with different levels of media consumption, we show that unfree media are partially effective in manipulating perceptions. Using age as an instrument for the time dedicated to media consumption, we find that higher levels of media freedom reduce citizen’s trust in government but are unrelated to satisfaction with national governments.
Blockage of the adenosine A2B receptor prevents cardiac fibroblasts overgrowth in rats with pulmonary arterial hypertension
Authors: Bessa-Gonçalves Mafalda , Bragança Bruno , Martins-Dias Eduardo , Vinhas Adriana , Certal Mariana , Rodrigues Tânia , Ferreirinha Fátima , Costa Maria Adelina , Correia-de-Sá Paulo , Fontes-Sousa Ana Patrícia
Published: July 2023

Abstract

Sustained pressure overload and fibrosis of the right ventricle (RV) are the leading causes of mortality in pulmonary arterial hypertension (PAH). Although the role of adenosine in PAH has been attributed to the control of pulmonary vascular tone, cardiac reserve, and inflammatory processes, the involvement of the nucleoside in RV remodelling remains poorly understood. Conflicting results exist on targeting the low-affinity adenosine A2B receptor (A2BAR) for the treatment of PAH mostly because it displays dual roles in acute vs. chronic lung diseases. Herein, we investigated the role of the A2BAR in the viability/proliferation and collagen production by cardiac fibroblasts (CFs) isolated from RVs of rats with monocrotaline (MCT)-induced PAH. CFs from MCT-treated rats display higher cell viability/proliferation capacity and overexpress A2BAR compared to the cells from healthy littermates. The enzymatically stable adenosine analogue, 5′-N-ethylcarboxamidoadenosine (NECA, 1–30 μM), concentration-dependently increased growth, and type I collagen production by CFs originated from control and PAH rats, but its effects were more prominent in cells from rats with PAH. Blockage of the A2BAR with PSB603 (100 nM), but not of the A2AAR with SCH442416 (100 nM), attenuated the proliferative effect of NECA in CFs from PAH rats. The A2AAR agonist, CGS21680 (3 and 10 nM), was virtually devoid of effect. Overall, data suggest that adenosine signalling via A2BAR may contribute to RV overgrowth secondary to PAH. Therefore, blockage of the A2AAR may be a valuable therapeutic alternative to mitigate cardiac remodelling and prevent right heart failure in PAH patients.
Income and Oral and General Health-Related Quality of Life: The Modifying Effect of Sense of Coherence, Findings of a Cross-Sectional Study
Authors: Zakershahrak Mehrsa , Chrisopoulos Sergio , Luzzi Liana , Jamieson Lisa , Brennan David
Published: July 2023

Objectives

To investigate whether a strong sense of coherence (SOC) modifies the association between low-income and oral and general health-related quality of life (OHRQoL and HRQoL, respectively) among a South Australian population sample; and to explore the main and interaction effects of income and SOC on OHRQoL and HRQoL.

Methods

Baseline data from the Dental Care and Oral Health Study (DCOHS, a South Australian representative study, 2015–2016) were used for cross-sectional analysis (n = 3,786). Four multivariable factorial ANOVA models were applied to assess the effect measure modification, main effects, and interaction of income and SOC on OHRQoL (measured using the OHIP-14) and HRQoL (measured using the EQ-5D-3L).

Results

Income and SOC had small main effects on OHRQoL. Income had a small effect and SOC had an intermediate effect on HRQoL, meaning that individuals with strong SOC had better OHRQoL and HRQoL in all income categories. Also, high-income participants had better OHRQoL and HRQoL. The interaction between income and SOC was statistically significant on HRQoL. Among participants from low-income group, those with strong SOC had better OHRQoL (mean = 8.8, 95% CI[7.9, 9.7]) and HRQoL (mean = 1.1, 95% CI[1.0, 1.3]) than others with weak SOC (OHIP-14 mean = 12.7, 95% CI[11.7, 13.6]) and (EQ-5D-3L mean = 2.0, 95% CI[1.9, 2.2]).

Conclusion

The findings showed the main effects and interaction between SOC and income on OHRQoL and HRQoL. Income had different effects on OHRQoL and HRQoL depending on whether SOC was strong or weak. Findings suggested that strong SOC modified the association between low-income and OHRQoL and HRQoL.
Screening native pepper, Tasmannia lanceolata (Poir.) A.C. Smith, for resistance against Phytophthora cinnamomi dieback
Authors: Sinhalagoda C. , Wilson M. D. , Tran S. N. , Cahill D. , Barry K. M.
Published: July 2023

Tasmannia lanceolata (native pepper) has been reported as susceptible to Phytophthora cinnamomi and the objective of this study was to identify variability in native pepper resistance to P. cinnamomi. Plant material was collected from native pepper populations across Tasmania (four regions) and selected commercially grown cultivars, and 47 clones were successfully propagated. Two disease screening experiments were conducted in “soil-free plant growth system” (SPS) units. Native pepper roots were inoculated with P. cinnamomi zoospores and maintained in controlled conditions. After one week, the pathogen was re-isolated to confirm infection success, and after two weeks root discolouration was visually assessed with images, which were then analysed with an automated machine learning system, using colour thresholds. The SPS was a successful approach to screen the early response of native pepper to P. cinnamomi. Based on pathogen re-isolation success and total root discolouration percentage, clones were categorised using a susceptibility rating method across multiple categories from highly resistant to highly susceptible. In the first experiment, all 47 propagated clones were challenged with one isolate of P. cinnamomi (Pc1), and pathogen re-isolation percentage and total root discolouration (brown and black) percentage differed significantly with clone (P < 0.001). In the second experiment, three representative clones of experiment 1 were challenged with two isolates of P. cinnamomi (Pc1 and Pc2) and clone response was similar. This study has highlighted that there is a range of responses to P. cinnamomi, from highly susceptible to highly resistant, in native pepper clones from different regions of Tasmania.
Ketogenic Diet as a Possible Non-pharmacological Therapy in Main Endocrine Diseases of the Female Reproductive System: A Practical Guide for Nutritionists
Authors: Camajani Elisabetta , Feraco Alessandra , Verde Ludovica , Moriconi Eleonora , Marchetti Marco , Colao Annamaria , Caprio Massimiliano , Muscogiuri Giovanna , Barrea Luigi
Published: July 2023

Purposeof Review

This narrative review explored the role of ketogenic diets (KDs) in improving fertility outcomes, low-grade inflammation, body weight, visceral adipose tissue, and its potential use in certain types of cancer, through its favorable actions on mitochondrial function, reactive oxygen species generation, chronic inflammation, and tumor growth inhibition.

Recent Findings 

Nutrition is crucial to maintain the female reproductive system’s health. Evidence on the association between diet and female reproductive system has greatly expanded over the last decade, leading to the identification of specific diet therapy, particularly KDs. KDs has been proved to be an effective weight-loss tool. To date, KDs is being increasingly used in the treatment of many diseases, such as obesity, type 2 diabetes mellitus. KDs is a dietary intervention capable of ameliorating the inflammatory state and oxidative stress through several mechanisms.

Due to the increasing use of KDs beyond obesity, this literature review will provide the latest scientific evidence of its possible use in common disorders of the female endocrine-reproductive tract, and a practical guide to its use in these patients.
Business Continuity Management, Operational Resilience, and Organizational Resilience: Commonalities, Distinctions, and Synthesis
Authors: Galaitsi S. E. , Pinigina Elizaveta , Keisler Jeffrey M. , Pescaroli Gianluca , Keenan Jesse M. , Linkov Igor
Published: July 2023

The concepts of business continuity management, operational resilience, and organizational resilience each refer to actions that businesses and organizations can take in anticipating and responding to disruptions. However, the existing definitions and usages are difficult to differentiate due to overlapping objectives, implementation processes, and outcomes. This article examines definitions and approaches for these three concepts and suggest a framework to operationalize methods and tools relevant to each. These definitions emphasize three dyads: risk versus resilience; organizational processes versus assets; and normal operating conditions versus crisis conditions. Using these dyads to differentiate the concepts of business continuity management, operational resilience, and organizational resilience can support planners in clarifying objectives and identifying which approach will be most beneficial as businesses or organizations plan for and encounter disruptions. This article evaluates these concepts by examining illustrative examples of disruptions and responses.