bluuebunny/crossref_metadata_2025 huggingface dataset:
Poly(Lactic Acid) Composites
Authors: Kaseem Mosab
Published: October 2019

Polylactic acid-based materials have gained great interest within the scientific community due to their biodegradability, good performance, and suitability for a number of applications. Therefore, this Special Issue “Poly(lactic acid) Composites” is proposed to cover the important advances in poly (lactic acid) composites, ranging from their design, fabrication, and material properties to the potential applications of these materials. Therefore, we believe that the present Issue can convey beneficial information to scientists and engineers in numerous fields, including polymer science and biomedical engineering.
Effects of the Quenching Rate on the Microstructure, Mechanical Properties and Paint Bake-Hardening Response of Al–Mg–Si Automotive Sheets
Authors: Gao Guanjun , Li Yong , Wang Zhaodong , Di Hongshuang , Li Jiadong , Xu Guangming
Published: October 2019

The quenching rate of Al–Mg–Si alloys during solution treatment is an important parameter for the automotive industry. In this work, the effect of the different quenching rates on the microstructures, mechanical properties, and paint bake-hardening response of Al–Mg–Si sheets was studied. Large dimples form on the fracture surface of a sample at a quenching rate of 0.01 °C/s. When the quenching rate increased to 58.9 °C/s, the dimples became smaller. The recrystallized grains and textures were slightly affected by quenching rates beyond 1.9 °C/s. Thus, higher r values of the samples were achieved with slower quenching rates. Furthermore, only the Al(FeMn)SiCr insoluble phases were observed in samples with a rapid quenching rate. Sufficient solute atoms and vacancies resulted in the improvement of the precipitation kinetics and paint bake-hardening capacity for Al–Mg–Si sheets at rapid rates. With a decrease in the quenching rate, the formation of the rod-like coarse β′ phases consumed many solute atoms and vacancies, leading to the deterioration of the paint bake-hardening capacity. This study provides a critical reference on quenching rates for industrial practices, so that good mechanical properties can be achieved using precision control of the quenching process.
Unusual Response of Thin LiTaO3 Films to Intense Microwave Pulses
Authors: Chen Haojia , Gao Qiong , Qian Baoliang , Zhao Lishan
Published: October 2019

Fundamentally different responses of a LiTaO 3 thin film detector are observed when it is subjected to short microwave pulses as the pulse intensity is altered over a wide range. We start from weak microwave pulses which lead to only trivial pyroelectric peak response. However, when the microwave pulses become intense, the normally expected pyroelectric signal seems to be suppressed and the sign of the voltage signal can even be completely changed. Analysis indicates that while the traditional pyroelectric model, which is a linear model and works fine for our data in the small regime, it does not work anymore in the large signal regime. Since the small-signal model is the key foundation of electromagnetic-wave sensors based on pyroelectric effects, such as pyroelectric infrared detecters, the observation in this work suggests that one should be cautious when using these devices in intense fields. In addition, the evolution of detector signal with respect to excitation strength suggests that the main polarisation process is changed in the large signal regime. This is of fundamental importance to the understanding on how crystalline solids interact with intense microwaves. Possible causes of the nonlinear behaviour is discussed.
Influence of Current Feeding Position of Duplex Current Feeding MIG Welding on Droplet Heat Quantity
Authors: Aoki Atsuhito , Tashiro Shinichi , Kurokawa Hideaki , Tanaka Manabu
Published: October 2019

Pure argon metal inert gas (MIG) welding is expected to offer the possibility to obtain high toughness weld joints. However, due to its arc instability and low wettability, it is difficult to apply pure argon MIG to a practical welding structure. In order to solve these problems, an improved MIG welding process with a duplex current feeding (DCF-MIG) mechanism was developed. In the DCF-MIG process, the welding current and the wire feeding speed are independently controlled by an additionally feeding secondary current from a secondary power source. Thereby, DCF-MIG can supply a large current compared to conventional MIG under the same deposition rate. In this study, to consider the influence of the secondary current feeding position of DCF-MIG on droplet heat quantity, droplet heat quantity was measured by calorimetry. As a result, the droplet heat quantity was found to be increased significantly with the increase of the distance between the primary current feeding point and secondary current feeding point. The increase of the droplet heat quantity in the DCF-MIG process had a strong effect on improving bead shape and penetration. The droplet heat quantity with the effective current value of DCF-MIG was derived from the simplified calculation and the results roughly agreed with the experimental data.
Mn-Doped BaTiO3 Ceramics: Thermal and Electrical Properties for Multicaloric Applications
Authors: Semenov Alexander , Dedyk Antonina , Mylnikov Ivan , Pakhomov Oleg , Es’kov Andrey , Anokhin Alexander , Krylov Vasiliy , Burovikhin Anton , Pavlova Yulia , Tselev Alexander , Kholkin Andrei
Published: October 2019

Multiferroic materials are widely used in microelectronics because they are sensitive to elastic, magnetic, and electric fields and there is an intrinsic coupling between them. In particular, transition metal-doped BaTiO3 is considered as a viable multiferroic because of the simultaneous presence of ferroelectricity and magnetism. In this work, we study the electrical and thermal properties of Mn-doped BaTiO3 ceramics that can be used for multicaloric applications. We found that Mn doping leads to the broadening and shifting of the phase transition accompanied with simultaneous decrease of latent heat and entropy. Mn doping causes a decrease in the bulk resistivity while contact resistance remains intact. Doped ceramics can withstand high electric fields (up to 40 kV/cm) and exhibit linear I-V characteristics followed by the Schottky limited current in contrast to earlier observations. As such, these ceramics are promising for multicaloric applications.
Design and Testing of a 3-DOF Robot for Studying the Human Response to Vibration
Authors: Marzaroli Pietro , Albanetti Alessandro , Negri Edoardo , Giberti Hermes , Tarabini Marco
Published: October 2019

This work describes the design and validation of an electro-mechanical excitation system for characterization of the response of the human body to multiaxial vibrations. The presented system is based on the linear delta configuration and is designed to expose standing subjects to vibration along three perpendicular axes, with an excitation bandwidth of at least 30 Hz and a maximum vibration amplitude of ±30 mm along the vertical direction and ±20 mm along the horizontal directions. The shaker characteristic dimensions are the result of numerical optimization of the inverse manipulability index; the motors and transmissions have been selected using a multibody dynamic simulation. Finite element simulations were performed to ensure that the structural resonances were outside the excitation bandwidth. Once the shaker had been manufactured, experiments were performed to verify the capability of the system in real testing conditions. The mean quadratic error between the modulus of the imposed acceleration and the measured one is between 5.7 × 10−3 and 1.4 × 10−2 m/s2 in the frequency range between 1 and 50 Hz, proving the good outcome of the design process.
Applying Undesirable Output Model to Security Evaluation of Taiwan
Authors: Wang Chia-Nan , Le Anh Luyen , Hou Chu-Chieh
Published: October 2019

The requirements and demand for personal security and public order have increased under great pressure from economic growth and society. This research applied the undesirable output model, which is a mathematical model, to measure the efficiencies of the security department in Taiwan. Further analysis has considered the efficient frontier to classify the efficiency of all 22 counties/cities in Taiwan in 2016, towards a sustainable security environment. The result of this research shown some cities have performed excellent efficiency in the security problem. According to analysis, the efficiency can be improved by decreasing excesses in inputs and bad outputs. This research has evaluated the police departments in Taiwan comprehensively and differentiated the efficiency in safety management of all police departments in Taiwan.
Investigation of the Possible Pharmacologically Active Forms of the Nicotinic Acetylcholine Receptor Agonist Anabaseine
Authors: Andrud Kristin , Xing Hong , Gabrielsen Bjarne , Bloom Linda , Mahnir Vladimir , Lee Stephen , Green Benedict T. , Lindstrom Jon , Kem William
Published: October 2019

Three major forms of the nicotinic agonist toxin anabaseine (cyclic iminium, cyclic imine and the monocationic open-chain ammonium-ketone) co-exist in almost equal concentrations at physiological pH. We asked the question: Which of these forms is pharmacologically active? First, we investigated the pH dependence of anabaseine inhibition of [3H]-methylcarbamylcholine binding at rat brain α4β2 nicotinic acetylcholine receptors (nAChRs). These experiments indicated that one or both monocationic forms interact with the orthosteric binding site for ACh. However, since they occur at equal concentrations near physiological pH, we employed another approach, preparing a stable analog of each form and examining its agonist activities and binding affinities at several vertebrate brain and neuromuscular nAChRs. Only 2-(3-pyridyl)-1,4,5,6-tetrahydropyrimidine monohydrogen chloride (PTHP), the cyclic iminium analog, displayed nAChR potencies and binding affinities similar to anabaseine. The cyclic imine analog 2,3′-bipyridyl and the open-chain ammonium-ketone analog 5-methylamino-1-(3-pyridyl)-1-pentanone (MAPP), displayed ≤1% of the activity predicted if the one form was solely active. The lower potency of weakly basic 2,3′-bipyridyl can be explained by the presence of a small concentration of its monocationic form. Since the open chain ammonium-ketone monocationic form of anabaseine has some structural similarity to the neurotransmitter GABA, we also tested the ability of anabaseine and its 1,2-dehydropyrrolidinyl analog myosmine to activate a mammalian GABAA receptor, but no activity was detected. We conclude that the monocationic cyclic iminium is the form which avidly binds and activates vertebrate nAChRs.
Dietary Supplementation with the Red Seaweed Porphyra umbilicalis Protects against DNA Damage and Pre-Malignant Dysplastic Skin Lesions in HPV-Transgenic Mice
Authors: Santos Susana , Ferreira Tiago , Almeida José , Pires Maria J. , Colaço Aura , Lemos Sílvia , Gil da Costa Rui M. , Medeiros Rui , Bastos Margarida M. S. M. , Neuparth Maria J. , Abreu Helena , Pereira Rui , Pacheco Mário , Gaivão Isabel , Rosa Eduardo , Oliveira Paula A.
Published: October 2019

Some diet profiles are associated with the risk of developing cancer; however, some nutrients show protective effects. Porphyra umbilicalis is widely consumed, having a balanced nutritional profile; however, its potential for cancer chemoprevention still needs comprehensive studies. In this study, we incorporated P. umbilicalis into the diet of mice transgenic for the human papillomavirus type 16 (HPV16), which spontaneously develop pre-malignant and malignant lesions, and determined whether this seaweed was able to block lesion development. Forty-four 20-week-old HPV+/− and HPV−/− mice were fed either a base diet or a diet supplemented with 10% seaweed. At the end of the study, skin samples were examined to classify HPV16-induced lesions. The liver was also screened for potential toxic effects of the seaweed. Blood was used to study toxicological parameters and to perform comet and micronucleus genotoxicity tests. P. umbilicalis significantly reduced the incidence of pre-malignant dysplastic lesions, completely abrogating them in the chest skin. These results suggest that P. umbilicalis dietary supplementation has the potential to block the development of pre-malignant skin lesions and indicate its antigenotoxic activity against HPV-induced DNA damage. Further studies are needed to establish the seaweed as a functional food and clarify the mechanisms whereby this seaweed blocks multistep carcinogenesis induced by HPV.
Virescenosides from the Holothurian-Associated Fungus Acremonium striatisporum Kmm 4401
Authors: Zhuravleva Olesya I. , Antonov Alexandr S. , Oleinikova Galina K. , Khudyakova Yuliya V. , Popov Roman S. , Denisenko Vladimir A. , Pislyagin Evgeny A. , Chingizova Ekaterina A. , Afiyatullov Shamil Sh.
Published: October 2019

Ten new diterpene glycosides virescenosides Z9-Z18 (1–10) together with three known analogues (11–13) and aglycon of virescenoside A (14) were isolated from the marine-derived fungus Acremonium striatisporum KMM 4401. These compounds were obtained by cultivating fungus on wort agar medium with the addition of potassium bromide. Structures of the isolated metabolites were established based on spectroscopic methods. The effects of some isolated glycosides and aglycons 15–18 on urease activity and regulation of Reactive Oxygen Species (ROS) and Nitric Oxide (NO) production in macrophages stimulated with lipopolysaccharide (LPC) were evaluated.
Bright Spots in the Darkness of Cancer: A Review of Starfishes-Derived Compounds and Their Anti-Tumor Action
Authors: Lazzara Valentina , Arizza Vincenzo , Luparello Claudio , Mauro Manuela , Vazzana Mirella
Published: October 2019

The fight against cancer represents a great challenge for researchers and, for this reason, the search for new promising drugs to improve cancer treatments has become inevitable. Oceans, due to their wide diversity of marine species and environmental conditions have proven to be precious sources of potential natural drugs with active properties. As an example, in this context several studies performed on sponges, tunicates, mollusks, and soft corals have brought evidence of the interesting biological activities of the molecules derived from these species. Also, echinoderms constitute an important phylum, whose members produce a huge number of compounds with diverse biological activities. In particular, this review is the first attempt to summarize the knowledge about starfishes and their secondary metabolites that exhibited a significant anticancer effect against different human tumor cell lines. For each species of starfish, the extracted molecules, their effects, and mechanisms of action are described.
Successful Approaches for a Red Seaweed Biorefinery
Authors: Álvarez-Viñas Milena , Flórez-Fernández Noelia , Torres M. Dolores , Domínguez Herminia
Published: October 2019

Macroalgae have been commercially exploited as food and for the production of phycocolloids, but they also contain compounds with potential in pharmaceutical, nutraceutical, cosmetic, chemical and energetic applications. The biorefinery concept applied to seaweed facilitates the extraction of different constituents ensuring full utilization of resources and generating few residues through a succession of steps. Seaweed biorefineries are less advanced than those based on terrestrial biomass and the design of efficient processes requires further study. This review presents practical successful examples to obtain two or more commercially valuable components from red seaweeds. The selected processes consist on cascading stages of both conventional and alternative techniques to illustrate different possible valorization strategies.
Improved Astaxanthin Production with Corynebacterium glutamicum by Application of a Membrane Fusion Protein
Authors: Henke Nadja A. , Wendisch Volker F.
Published: October 2019

Astaxanthin is one of the strongest natural antioxidants and a red pigment occurring in nature. This C40 carotenoid is used in a broad range of applications such as a colorant in the feed industry, an antioxidant in cosmetics or as a supplement in human nutrition. Natural astaxanthin is on the rise and, hence, alternative production systems are needed. The natural carotenoid producer Corynebacterium glutamicum is a potent host for industrial fermentations, such as million-ton scale amino acid production. In C. glutamicum, astaxanthin production was established through heterologous overproduction of the cytosolic lycopene cyclase CrtY and the membrane-bound β-carotene hydroxylase and ketolase, CrtZ and CrtW, in previous studies. In this work, further metabolic engineering strategies revealed that the potential of this GRAS organism for astaxanthin production is not fully exploited yet. It was shown that the construction of a fusion protein comprising the membrane-bound β-carotene hydroxylase and ketolase (CrtZ~W) significantly increased astaxanthin production under high glucose concentration. An evaluation of used carbon sources indicated that a combination of glucose and acetate facilitated astaxanthin production. Moreover, additional overproduction of cytosolic carotenogenic enzymes increased the production of this high value compound. Taken together, a seven-fold improvement of astaxanthin production was achieved with 3.1 mg/g CDW of astaxanthin.
Sediment as a Potential Pool for Lipophilic Marine Phycotoxins with the Case Study of Daya Bay of China
Authors: Liu Yang , Zhang Peng , Du Sen , Lin Zhuoru , Zhou Yanyan , Chen Lizhao , Yu Rencheng , Zhang Li
Published: October 2019

Marine sediments can reserve many environmental pollutants. Lipophilic marine phycotoxins (LMPs) are natural toxic substances widespread in the marine environment; however, evidence of their existence in sediment is scarce. In the present study, in order to explore the occurrence and distribution characteristics of LMPs in sediment, surface sediment samples collected from a tropical area of Daya Bay (DYB) at different seasons, were analyzed using liquid chromatography with tandem mass spectrometry (LC-MS/MS). According to the results, up to six toxin compounds were detected in sediment samples from DYB, OA and DTX1 had the highest levels, followed by PTX2, homo-YTX, AZA2, and GYM. Although AZA2 and GYM were found in most of the sediment, OA, DTX1, homo-YTX, and PTX2 were the predominant toxin compounds, and PTX2 was the most ubiquitous toxin in sediment. The spatial distribution of LMP components in the sediment fluctuated with sampling times, partially according to the physical–chemical parameters of the sediment. There are likely several sources for LMPs existing in surface sediments, but it is difficult to determine contributions of a specific toxin-source in the sediment. Therefore, marine sediments may be a toxin reservoir for LMPs accumulation in benthic organisms via food chains.
Performance Comparison of a Laterally-Fed Membrane Chromatography (LFMC) Device with a Commercial Resin Packed Column
Authors: Madadkar Pedram , Sadavarte Rahul , Ghosh Raja
Published: October 2019

The use of conventional membrane adsorbers such as radial flow devices is largely restricted to flow-through applications, such as virus and endotoxin removal, as they fail to give acceptable resolution in bind-and-elute separations. Laterally-fed membrane chromatography or LFMC devices have been specifically developed to combine high-speed with high-resolution. In this study, an LFMC device containing a stack of strong cation exchange membranes was compared with an equivalent resin packed column. Preliminary characterization experiments showed that the LFMC device had a significantly greater number of theoretical plates per metre than the column. These devices were used to separate a ternary model protein mixture consisting of ovalbumin, conalbumin and lysozyme. The resolution obtained with the LFMC device was better than that obtained with the column. For instance, the LFMC device could resolve lysozyme dimer from lysozyme monomer, which was not possible using the column. In addition, the LFMC device could be operated at lower pressure and at significantly higher flow rates. The devices were then compared based on an application case study, i.e., preparative separation of monoclonal antibody charge variants. The LFMC device gave significantly better separation of these variants than the column.
Highly Conductive and Flexible Gel Polymer Electrolyte with Bis(Fluorosulfonyl)imide Lithium Salt via UV Curing for Li-Ion Batteries
Authors: Jin Lei , Ahmed Faiz , Ryu Taewook , Yoon Sujin , Zhang Wei , Lee Yonghoon , Kim Daeho , Jang Hohyoun , Kim Whangi
Published: October 2019

A series of new self-standing gel polymer electrolytes (SGPEs) were fabricated by ultraviolet (UV) curing and investigated for application in flexible lithium-ion batteries. Compared with traditional gel polymer electrolytes (combine with solvents or plasticizers), these new SGPEs were prepared simply by curing different weight ratios of lithium bis(fluorosulfonyl)imide (LiFSI) with a methacrylic linear monomer, poly (ethylene glycol) dimethacrylate (PEGDMA). Noticeably, there were no solvents or plasticizers combined with the final SGPEs. Owing to this, the SGPEs showed high flexibility and strong mechanical stability. Some paramount physicochemical and electrochemical characters were observed. The SGPEs demonstrated good thermal stability below 150 °C and an extremely low glass transition temperature (Tg) (around −75 °C). Moreover, plastic crystal behaviors were also identified in this study. Ultimately, the SGPEs demonstrated excellent ionic conductivity at room temperature, which proves that these new SGPEs could be widely applied as a prospective electrolyte in flexible lithium-ion batteries.
Antituberculosis Drug Interactions with Membranes: A Biophysical Approach Applied to Bedaquiline
Authors: Pinheiro Marina , Amenitsch Heinz , Reis Salette
Published: October 2019

This work focuses on the interaction of the novel and representative antituberculosis (anti-TB) drug bedaquiline (BDQ) with different membrane models of eukaryotic and prokaryotic cells. The effect of BDQ on eukaryotic cell membrane models was assessed using liposomes, namely, multilamellar vesicles (MLVs) made of 1,2-dimyristoyl-rac-glycero-3-phosphocholine (DMPC) and also a mixture of DMPC and cholesterol (CHOL) (8:2 molar ratio). To mimic the prokaryotic cell membrane, 1,2-dimyristoyl-sn-glycero-3-phospho-rac-(1-glycerol) (DMPG) and 1,1′2,2′-tetra-oleoyl-cardiolipin (TOCL) were chosen. Powerful biophysical techniques were employed, including small-angle X-ray scattering (SAXS) and wide-angle X-ray scattering (WAXS), to understand the effect of BDQ on the nanostructure of the membrane models. The results showed that BDQ demonstrated a pronounced disordering effect in the bacterial cell membrane models, especially in the membrane model with cardiolipin (CL), while the human cell membrane model with large fractions of neutral phospholipids remained less affected. The membrane models and techniques provide detailed information about different aspects of the drug–membrane interaction, thus offering valuable information to better understand the effect of BDQ on their target membrane-associated enzyme as well as its side effects on the cardiovascular system.
The Effect of Milling Cooling Conditions on the Surface Integrity and Fatigue Behavior of the GH4169 Superalloy
Authors: Xu Rufeng , Zhou Yongxin , Li Xun , Yang Shenliang , Han Kangning , Wang Shijun
Published: October 2019

The GH4169 superalloy has high strength at high temperatures. Cooling conditions have a major impact on the machined surface integrity, which further affects the fatigue properties of specimens of the GH4169 superalloy. The influence of cooling conditions on the surface integrity of the GH4169 superalloy is first studied during the side milling. Then, the effect of surface integrity under different cooling conditions on the fatigue behavior of specimens of the GH4169 superalloy is investigated by a standard tensile and tensile–mode fatigue testing. The results obtained show that surface roughness and the depth of the plastic deformation layer in wet milling and dry milling makes little difference, the surface microhardness rate in dry milling is slightly lower than that in wet milling, the surface tensile residual stress in dry milling is significantly higher than that in wet milling, and the fatigue behavior in dry milling is only about 50% of that in wet milling. In addition, the crack initiation of specimens of the GH4169 superalloy utilizing wet milling is on the subsurface, while that from dry milling is on the surface. Thus, cooling conditions have an important impact on the fatigue behavior of specimens of the GH4169 superalloy, and micro defects in dry milling are the main factors of decreasing of fatigue behavior of specimens of the GH4169 superalloy.
Quantification of Urinary Phenyl-γ-Valerolactones and Related Valeric Acids in Human Urine on Consumption of Apples
Authors: Anesi Andrea , Mena Pedro , Bub Achim , Ulaszewska Marynka , Del Rio Daniele , Kulling Sabine E. , Mattivi Fulvio
Published: October 2019

Flavan-3-ols are dietary bioactive molecules that have beneficial effects on human health and reduce the risk of various diseases. Monomeric flavan-3-ols are rapidly absorbed in the small intestine and released in the blood stream as phase II conjugates. Polymeric flavan-3-ols are extensively metabolized by colonic gut microbiota into phenyl-γ-valerolactones and their related phenylvaleric acids. These molecules are the main circulating metabolites in humans after the ingestion of flavan-3-ol rich-products; nevertheless, they have received less attention and their role is not understood yet. Here, we describe the quantification of 8 phenyl-γ-valerolactones and 3 phenylvaleric acids in the urine of 11 subjects on consumption of apples by using UHPLC-ESI-Triple Quad-MS with pure reference compounds. Phenyl-γ-valerolactones, mainly as sulfate and glucuronic acid conjugates, reached maximum excretion between 6 and 12 after apple consumption, with a decline thereafter. Significant differences were detected in the cumulative excretion rates within subjects and in the ratio of dihydroxyphenyl-γ-valerolactone sulfate to glucuronide conjugates. This work observed for the first time the presence of two distinct metabotypes with regards to the excretion of phenyl-γ-valerolactone phase II conjugates.
Ultra-Sensitive Flexible Tactile Sensor Based on Graphene Film
Authors: Lü Xiaozhou , Qi Liang , Hu Hanlun , Li Xiaoping , Bai Guanghui , Chen Jun , Bao Weimin
Published: October 2019

Flexible tactile sensor can be integrated into artificial skin and applied in industrial robot and biomedical engineering. However, the presented tactile sensors still have challenge in increasing sensitivity to expand the sensor’s application. Aiming at this problem, this paper presents an ultra-sensitive flexible tactile sensor. The sensor is based on piezoresistive effect of graphene film and is composed of upper substrate (PDMS bump with a size of 5 mm × 7 mm and a thickness of 1 mm), medial Graphene/PET film (Graphene/PET film with a size of 5 mm × 7 mm, PET with a hardness of 2H) and lower substrate (PI with fabricated electrodes). We presented the structure and reduced the principle of the sensor. We also fabricated several sample devices of the sensor and carried out experiment to test the performance. The results show that the sensor performed an ultra high sensitivity of 10.80/kPa at the range of 0–4 kPa and have a large measurement range up to 600 kPa. The sensor has 4 orders of magnitude between minimum resolution and maximum measurement range which have great advantage compared with state of the art. The sensor is expected to have great application prospect in robot and biomedical.
Microfluidic Magnetic Mixing at Low Reynolds Numbers and in Stagnant Fluids
Authors: Shanko Eriola-Sophia , van de Burgt Yoeri , Anderson Patrick D. , den Toonder Jaap M. J.
Published: October 2019

Microfluidic mixing becomes a necessity when thorough sample homogenization is required in small volumes of fluid, such as in lab-on-a-chip devices. For example, efficient mixing is extraordinarily challenging in capillary-filling microfluidic devices and in microchambers with stagnant fluids. To address this issue, specifically designed geometrical features can enhance the effect of diffusion and provide efficient mixing by inducing chaotic fluid flow. This scheme is known as “passive” mixing. In addition, when rapid and global mixing is essential, “active” mixing can be applied by exploiting an external source. In particular, magnetic mixing (where a magnetic field acts to stimulate mixing) shows great potential for high mixing efficiency. This method generally involves magnetic beads and external (or integrated) magnets for the creation of chaotic motion in the device. However, there is still plenty of room for exploiting the potential of magnetic beads for mixing applications. Therefore, this review article focuses on the advantages of magnetic bead mixing along with recommendations on improving mixing in low Reynolds number flows (Re ≤ 1) and in stagnant fluids.
Fabrication of TiO2-Nanotube-Array-Based Supercapacitors
Authors: Ahmed Faheem , Pervez Syed A. , Aljaafari Abdullah , Alshoaibi Adil , Abuhimd Hatem , Oh JooHyeon , Koo Bon Heun
Published: October 2019

In this work, a simple and cost-effective electrochemical anodization technique was adopted to rapidly grow TiO2 nanotube arrays on a Ti current collector and to utilize the synthesized materials as potential electrodes for supercapacitors. To accelerate the growth of the TiO2 nanotube arrays, lactic acid was used as an electrolyte additive. The as-prepared TiO2 nanotube arrays with a high aspect ratio were strongly adhered to the Ti substrate. X-ray diffraction (XRD) and transmission electron microscopy (TEM) results confirmed that the TiO2 nanotube arrays were crystallized in the anatase phase. TEM images confirmed the nanotublar-like morphology of the TiO2 nanotubes, which had a tube length and a diameter of ~16 and ~80 nm, respectively. The electrochemical performance of the TiO2 nanotube array electrodes was evaluated using the cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and galvanostatic charge/discharge (GCD) measurements. Excellent electrochemical response was observed for the electrodes based on the TiO2 nanotube arrays, as the cells delivered a high specific capacitance of 5.12 mF/cm2 at a scan rate of 100 mV/s and a current density of 100 µA/cm2. The initial capacity was maintained for more than 250 cycles. Further, a remarkable rate capability response was observed, as the cell retained 88% of the initial areal capacitance when the scan rate was increased from 10 to 500 mV/s. The results suggest the suitability of TiO2 nanotube arrays as electrode materials for commercial supercapacitor applications.
A Novel Capacitance-Based In-Situ Pressure Sensor for Wearable Compression Garments
Authors: Lao Steven , Edher Hamza , Saini Utkarsh , Sixt Jeffrey , Salehian Armaghan
Published: October 2019

This paper pertains to the development & evaluation of a dielectric electroactive polymer-based tactile pressure sensor and its circuitry. The evaluations conceived target the sensor’s use case as an in-situ measurement device assessing load conditions imposed by compression garments in either static form or dynamic pulsations. Several testing protocols are described to evaluate and characterize the sensor’s effectiveness for static and dynamic response such as repeatability, linearity, dynamic effectiveness, hysteresis effects of the sensor under static conditions, sensitivity to measurement surface curvature and temperature and humidity effects. Compared to pneumatic sensors in similar physiological applications, this sensor presents several significant advantages including better spatial resolution, compact packaging, manufacturability for smaller footprints and overall simplicity for use in array configurations. The sampling rates and sensitivity are also less prone to variability compared to pneumatic pressure sensors. The presented sensor has a high sampling rate of 285 Hz that can further assist with the physiological applications targeted for improved cardiac performance. An average error of ± 5.0 mmHg with a frequency of 1–2 Hz over a range of 0 to 120 mmHg was achieved when tested cyclically.
Design and Optimization of Germanium-Based Gate-Metal-Core Vertical Nanowire Tunnel FET
Authors: Jang Won Douk , Yoon Young Jun , Cho Min Su , Jung Jun Hyeok , Lee Sang Ho , Jang Jaewon , Bae Jin-Hyuk , Kang In Man
Published: October 2019

In this paper, a germanium-based gate-metal-core vertical nanowire tunnel field effect transistor (VNWTFET) has been designed and optimized using the technology computer-aided design (TCAD) simulation. In the proposed structure, by locating the gate-metal as a core of the nanowire, a more extensive band-to-band tunneling (BTBT) area can be achieved compared with the conventional core–shell VNWTFETs. The channel thickness (Tch), the gate-metal height (Hg), and the channel height (Hch) were considered as the design parameters for the optimization of device performances. The designed gate-metal-core VNWTFET exhibits outstanding performance, with an on-state current (Ion) of 80.9 μA/μm, off-state current (Ioff) of 1.09 × 10−12 A/μm, threshold voltage (Vt) of 0.21 V, and subthreshold swing (SS) of 42.8 mV/dec. Therefore, the proposed device was demonstrated to be a promising logic device for low-power applications.
Defective RNA of a Novel Mycovirus with High Transmissibility Detrimental to Biocontrol Properties of Trichoderma spp.
Authors: You Jiaqi , Zhou Kang , Liu Xiaolin , Wu Mingde , Yang Long , Zhang Jing , Chen Weidong , Li Guoqing
Published: October 2019

Trichoderma species are a group of fungi which is widely distributed in major terrestrial ecosystems; they are also commonly used as biocontrol agents for many plant diseases. A virus, namely Trichoderma harzianum hypovirus 1 (ThHV1), was identified in T. harzianum isolate T-70, and also infected isolate T-70D, together with its defective RNA (ThHV1-S). The ThHV1 genome possessed two Open Reading Frames (ORFs), namely ORF1 and ORF2. The start codon of ORF2 overlapped with the stop codon of ORF1 in a 43 nt long region. The polypeptide encoded by ORF2 of ThHV1 shared sequence similarities with those of betahypoviruses, indicating that ThHV1 is a novel member of Hypoviridea. Isolate T-70D, carrying both ThHV1 and ThHV1-S, showed abnormal biological properties, notably a decreased mycoparasitism ability when compared with isolate T-70. Both ThHV1 and ThHV1-S could be vertically transmitted to conidia and horizontally transmitted to T. harzianum isolate T-68 and T. koningiopsis T-51. The derivative strains carrying both ThHV1 and ThHV1-S showed decreased mycoparasitism ability, whereas strains carrying ThHV1 alone were normal, indicating that ThHV1-S is closely associated with the decreased mycoparasitism ability of T. harzianum isolate T-70D. ThHV1 was widely detected in isolates of T. harzianum, T. koningiopsis and T. atroviride originating from soil of China. Therefore, viruses in fungal biocontrol agents may also be a factor associated with the stability of their application.
Red-Brown Pigmentation of Acidipropionibacterium jensenii Is Tied to Haemolytic Activity and cyl-Like Gene Cluster
Authors: Deptula Paulina , Loivamaa Iida , Smolander Olli-Pekka , Laine Pia , Roberts Richard J. , Piironen Vieno , Paulin Lars , Savijoki Kirsi , Auvinen Petri , Varmanen Pekka
Published: October 2019

The novel Acidipropionibacterium genus encompasses species of industrial importance but also those associated with food spoilage. In particular, Acidipropionibacterium acidipropionici, Acidipropionibacterium thoenii, and Acidipropionibacterium jensenii play an important role in food fermentation, as biopreservatives, or as potential probiotics. Notably, A. jensenii and A. thoenii can cause brown spot defects in Swiss-type cheeses, which have been tied to the rhamnolipid pigment granadaene. In the pathogenic bacterium Streptococcus agalactiae, production of granadaene depends on the presence of a cyl gene cluster, an important virulence factor linked with haemolytic activity. Here, we show that the production of granadaene in pigmented Acidipropionibacterium, including A. jensenii, A. thoenii, and Acidipropionibacterium virtanenii, is tied to haemolytic activity and the presence of a cyl-like gene cluster. Furthermore, we propose a PCR-based test, which allows pinpointing acidipropionibacteria with the cyl-like gene cluster. Finally, we present the first two whole genome sequence analyses of the A. jensenii strains as well as testing phenotypic characteristics important for industrial applications. In conclusion, the present study sheds light on potential risks associated with the presence of pigmented Acidipropionibacterium strains in food fermentation. In addition, the results presented here provide ground for development of a quick and simple diagnostic test instrumental in avoiding potential negative effects of Acidipropionibacterium strains with haemolytic activity on food quality.
Characterization of Rock Samples by A High-Resolution Multi-Technique Non-Invasive Approach
Authors: Fais Silvana , Cuccuru Francesco , Casula Giuseppe , Bianchi Maria Giovanna , Ligas Paola
Published: October 2019

Three different non-invasive techniques, namely Structure from Motion (SfM) photogrammetry, Terrestrial Laser Scanner (TLS) and ultrasonic tomography integrated with petrographic data, were applied to characterize two rock samples of a different nature: A pyroclastic rock and a carbonate rock. We started a computation of high-resolution 3D models of the two samples using the TLS technique supported by a digital SfM photogrammetry survey. The resulting radiometric information available, such as reflectivity maps, SfM photogrammetry textured models and patterns of geometrical residuals, were interpreted in order to detect and underline surface materials anomalies by a comparison of reflectance and natural colour anomalies. Starting from the 3D models from previous techniques, a 3D ultrasonic tomography on each rock sample was accurately planned and carried out in order to detect internal defects or sample heterogeneity. The integration of the above three geophysical non-invasive techniques with petrographical data—especially with the textural characteristics of such materials—represents a powerful method for the definition of the heterogeneity of the rocks at a different scale and for calibrating in situ measurements.
Effects and Mechanism of Different Grinding Media on the Flotation Behaviors of Beryl and Spodumene
Authors: Hu Zhenfeng , Sun Chuanyao
Published: October 2019

The flotation behavior of beryl and spodumene (typical silicate minerals) was studied at wet-grinding conditions with different grinding mediums, with dodecylamine or sodium oleate as the collector. The mechanism of potential influence of the grinding medium to the flotation behavior was investigated through measuring zeta potential of pure minerals, micro scanning by X-ray photoelectron spectroscopy (XPS), surface interaction simulation and etc. The test data suggested that, with dodecylamine as the collector and relatively lower pH, the recoveries of beryl and spodumene are higher with zircon balls as wet-grinding media than those with iron balls, while in the higher pH, the recovery difference became smaller. With sodium oleate as a collector, within the same pH environment, the recovery of beryl and spodumene under zircon ball wet-grinding is lower than those under iron wet-grinding. We observed formation of iron-hydroxyl complexes on the mineral surface after iron ball grinding, which are significant influence on the flotation behavior of silicate minerals. Furthermore, for iron wet-grinding, the iron adsorbed on the mineral surface increased the adsorption energy between laurylamine (cationic collector) and silicate minerals, which inhibited the interaction between collectors and minerals. On the other hand, the interaction energy between sodium oleate (anion collector) and silicate minerals was decreased, which promoted the interaction between collectors and minerals.
New Constraints on the Main Mineralization Event Inferred from the Latest Discoveries in the Bor Metallogenetic Zone (BMZ, East Serbia)
Authors: Banješević Miodrag , Cvetković Vladica , von Quadt Albrecht , Ljubović Obradović Darivojka , Vasić Nebojša , Pačevski Aleksandar , Peytcheva Irena
Published: October 2019

This study aims at better constraining the link between magmatism and metallogeny in the south-easternmost sector of the Bor Metallogenetic Zone (BMZ), where the world-class copper and gold deposit of Čukaru Peki was recently discovered. The obtained U/Pb zircon ages confirm the earlier knowledge that the major Cu–Au porphyry and epithermal mineralization in the BMZ is genetically related to the first volcanic phase (‘Timok andesite’; 85–90 Ma). However, the data also suggest that during this phase, two subgroups of andesite porphyry were formed; they are named volcanic phase 1A (V1A) and volcanic phase 1B (V1B). The V1A andesite (89–90 Ma) is plagioclase-hornblende phyric, holocrystalline and ubiquitously hydrothermally altered and/or mineralized, whereas the V1B (85–86 Ma) is hornblende-plagioclase phyric, holo- to hypocrystalline, fresh, and non-mineralized. According to our simplified model, the contrasting productivity of the V1A and V1B is explained by fluctuations during AFC (assimilation-fractional crystallization) processes of water-rich parental magma, which have controlled the order of crystallization of hornblende and plagioclase in the V1A and V1B andesite.
Geochemical Fractions of the Agricultural Soils of Southern Poland and the Assessment of the Potentially Harmful Element Mobility
Authors: Gruszecka-Kosowska Agnieszka , Baran Agnieszka , Mazur-Kajta Katarzyna , Czech Tomasz
Published: October 2019

Surface samples (0–25 cm each) of agricultural soils were investigated in five Regions (voivodeships) of southern Poland. The mean Potentially Harmful Element (PHE) pseudototal content ranges were as follows (mg/kg): As 5.19–10.9, Cd 0.34–1.56, Co 1.92–6.70, Cr 9.05–25.7, Cu 8.74–69.4, Hg 0.001–0.08, Ni 3.93–19.9, Pb 20.3–183, Sb 0.80–1.42, Tl 0.04–0.17, and Zn 61.3–422. The PHE availability depended on pH, the organic carbon (Corg) content, and the pseudototal PHE content in soils. Exchangeable and acid soluble PHE contents (BCRF1) determined in the Community Bureau of Reference (BCR) three-step sequential extraction procedure decreased in this order: Cd > Zn > Co > Ni = Sb > Cu > Tl > As > Cr = Pb. Actually available PHE contents in pore water (0.01 mol/dm3 CaCl2) ranged as follows: Cd 0.81–17%, Cr 0–0.25%, Cu 0.01–2.31%, Ni 0.16–2%, Pb 0.2–0.49%, and Zn 0.25–2.12%. The potential soluble total content of PHEs in pore water (0.05 mol/dm3 Na2EDTA) ranged as follows: Cd 27–91%, Cr 0.7–7.1%, Cu 6.7–98%, Ni 3.6–41%, Pb 15–41%, and Zn 3–34%. The mobility factor (MF) values indicated Cd (31.6%) and Zn (21.0%) as the most mobile elements in soil. Other PHEs followed the order of Co > Ni > Tl > As > Sb > Cu > Cr > Pb, with the MF values <10%. The risk assessment code (RAC) values revealed a very high ecological risk of Cd and Zn in the Podkarpackie Region and a high ecological risk of Cd in the Regions of Opolskie, Śląskie, Małopolskie, and Podkarpackie, and the same of Zn in the Opolskie and Śląskie. The modified risk assessment code (mRAC) index pointed a very high potential of adverse effects in soils in the Podkarpackie and a medium potential in the Opolskie, Śląskie, Małopolskie, and Świętokrzyskie. The potential adverse effect risk, described by the individual contamination factor (ICF) factor, was the following in the Regions, in the decreasing order: Cd > Pb > Sb > Zn > Co > Cu > Ni > Tl > As > Cr, and the same as described by the global contamination factor (GCF) values: Opolskie > Podkarpackie > Świętokrzyskie > Śląskie > Małopolskie.
Anti-Cancer and Ototoxicity Characteristics of the Curcuminoids, CLEFMA and EF24, in Combination with Cisplatin
Authors: Monroe Jerry D. , Hodzic Denis , Millay Matthew H. , Patty Blaine G. , Smith Michael E.
Published: October 2019

In this study, we investigated whether the curcuminoids, CLEFMA and EF24, improved cisplatin efficacy and reduced cisplatin ototoxicity. We used the lung cancer cell line, A549, to determine the effects of the curcuminoids and cisplatin on cell viability and several apoptotic signaling mechanisms. Cellular viability was measured using the MTT assay. A scratch assay was used to measure cell migration and fluorescent spectrophotometry to measure reactive oxygen species (ROS) production. Western blots and luminescence assays were used to measure the expression and activity of apoptosis-inducing factor (AIF), caspases-3/7, -8, -9, and -12, c-Jun N-terminal kinases (JNK), mitogen-activated protein kinase (MAPK), and proto-oncogene tyrosine-protein kinase (Src). A zebrafish model was used to evaluate auditory effects. Cisplatin, the curcuminoids, and their combinations had similar effects on cell viability (IC50 values: 2–16 μM) and AIF, caspase-12, JNK, MAPK, and Src expression, while caspase-3/7, -8, and -9 activity was unchanged or decreased. Cisplatin increased ROS yield (1.2-fold), and curcuminoid and combination treatments reduced ROS (0.75–0.85-fold). Combination treatments reduced A549 migration (0.51–0.53-fold). Both curcuminoids reduced auditory threshold shifts induced by cisplatin. In summary, cisplatin and the curcuminoids might cause cell death through AIF and caspase-12. The curcuminoids may potentiate cisplatin’s effect against A549 migration, but may counteract cisplatin’s effect to increase ROS production. The curcuminoids might also prevent cisplatin ototoxicity.
Brominated Bisindole Alkaloids from the Celtic Sea Sponge Spongosorites calcicola
Authors: Jennings Laurence K. , Khan Neyaz M. D. , Kaur Navdeep , Rodrigues Daniel , Morrow Christine , Boyd Aoife , Thomas Olivier P.
Published: October 2019

As part of an ongoing program to identify new bioactive compounds from Irish marine bioresources, we selected the subtidal sponge Spongosorites calcicola for chemical study, as fractions of this species displayed interesting cytotoxic bioactivities and chemical profiles. The first chemical investigation of this marine species led to the discovery of two new bisindole alkaloids of the topsentin family, together with six other known indole alkaloids. Missing the usual central core featured by the representatives of these marine natural products, the new metabolites may represent key biosynthetic intermediates for other known bisindoles. These compounds were found to exhibit weak cytotoxic activity against HeLa tumour cells, suggesting a specificity towards previously screened carcinoma and leukaemia cells.