bluuebunny/crossref_metadata_2025 huggingface dataset:
The Research into Quality Control over Bolt Support for High Slopes
Authors: Deng Hua
Published: November 2019

The height of slope has a significant impact on its safety and stability, for which an effective quality control enforced over the course of construction is essential to ensure the safety of construction works. In this paper, the high-slope bolt involved in a specific engineering project is exemplified to analyze the quality-related issues, based on which the major influencing factors in construction quality are identified as the techniques and environment of construction. Further with this, an analytical research is conducted into the quality of construction with three priority procedures, including the construction of latticed girder, bolt and slope concrete spraying. The construction environment shall be subjected to effective control and the ultimate quality of construction shall be inspected as compliant. In doing so, valuable reference could be provided for the engineering works of the same category in terms of construction.
Study on orthogonal test of plateau frozen soil under dynamic load
Authors: Wen Dongyao , Jiang Ningshan , Li Hongwei , Zhang Wuyu , Li Hu
Published: November 2019

With the increase in global temperature, the frozen soil in the Qinghai-Tibet Plateau has been degraded to varying degrees, resulting in frequent road disease in the cold area, causing the bearing capacity of the frozen soil subgrade to be attenuated. In order to study the effects of the elevated temperature on the deviatoric stress and strain of the subgrade soil, the loading rate, dynamic stress amplitude, and frequency were introduced to simulate the working conditions of the highway, which ensure the negative effect of loading rate, dynamic stress amplitude, and frequency on the deviatoric stress. Then, using the orthogonal test method, for the 9 samples make the GDS dynamic triaxial test. The test results showed that the vibration frequency is the major effect factor on the deviatoric stress, deviatoric stress increased with the vibration frequency increased; the strain increased with the loading rate increased.
Influencing factor analysis and control measures for speedy excavation in fully mechanized mining roadway
Authors: Feng Tantan , Zhu Xinming , Xie Xiaohua , Zhang Hao
Published: November 2019

To solve the existing problems, such as low mining rate of coal and tension mining replacement triggered by slow roadway excavation in Linyi Mining, this paper analyzed the main influenced factors and control methods of the speedy excavation of tunnel via comprehensive investigation method, taking the transportation roadway in the second mining area as an example of specific engineering geological conditions. The research shows that the main influencing factors include complex geological structure, unreasonable support of design scheme and working procedure, complex transportation system and defective personnel and equipment management. Based on those factors, the control measures, such as strengthening geological prediction, optimizing the construction process and auxiliary transportation system as well as innovating management concepts, were proposed, which has effectively solved the current problems and provided guidance and reference for the similar speedy excavation projects.
Study on fire area unsealing method in high gas outburst mine
Authors: Zhang Qingsong
Published: November 2019

The unsealing fire zone is very particular of the in the high-gas outburst mine. By analyzing the limitation of the ventilation unsealing method and the reduced ventilation unsealing method during unsealing fire area in the high-gas outburst mine, it is proposed to use the gas drainage system to unseal. It also is proposed to strengthen gas index analysis in the closed deep fire zone in order to improve the verdict accuracy of fire extinguish judgment. And it can speed up the safety process of high concentration gas in the unsealed area. By adjustment the ventilation system, mine rescue team in the process of unsealing the fire area is always in the fresh air space, which avoid the vicious incidents of gas explosion during the wind tunnel closed wall unsealing in order to protect the personal safety of the team members.
Optimization of Chlorpyrifos Degradation Conditions for and Microbial Community Structure Analysis in a Constructed Wetland System
Authors: Yanji Wang , Songhe Jiang , Wenhua Piao , Mingji Jin , Yunjiang Liang
Published: November 2019

In this study, chlorpyrifos degradation under different operating conditions was examined in a constructed wetland system, and the degradation conditions were optimized. Additionally, the microbial community structure in wetland system was analysed by high-throughput sequencing. The results are that the best chlorpyrifos degradation conditions in the constructed wetland system were pH = 8.69, sand/natural soil ratio of 1.36:1, and a planting density of 173.73/m2. The actual chlorpyrifos degradation rate was 75.68%. Moreover, Proteobacteria, Bacteroidetes and Actinobacteria; Gemmatimonas, Gp6 and Parcubacteria and so on had gradually become the main chlorpyrifos degraders of dominant phylum and genera with extension in a constructed wetland system.
The Influence and Integrated Governance Planning of Coal Mining Subsidence Areas in Hegang City
Authors: Jiang Yun , Li Yanjun , Zhang Guanzhuang
Published: November 2019

In this paper, the impact of coal mining subsidence on geological ecology, road traffic, shantytown renovation and industrial park production and construction is analyzed. With the goal of promoting economic development, ensuring farmers’ income and improving the ecological environment, the three innovative concepts of economic engine, people’s livelihood security, and urban green lungs’ comprehensive management plan for coal mining subsidence area are put forward. On this basis, the zoning integrated governance planning strategy of “one corridor and four districts” is expounded, namely, ecological cycle industry corridor, sightseeing and tourism area, ecological agriculture area, modern industrial area and landscape leisure area. The implementation measures of integrated control programming are discussed from the aspects of state funds or policy support and corporate performance obligations, in order to provide reference for the integrated governance planning of coal mining subsidence in Hegang city.
Comparison of Cold Resistance between Aboveground and Underground of Sedum aizoon L. Seedling with Sprout under Low Temperature
Authors: Chen Yan , Wang Haixia , He Zhongqun
Published: November 2019

The difference of cold resistance between aboveground and underground parts was compared by measuring the growth and cold-resistance physiology of dormancy-released Sedum aizoon L. seedling with sprout at -5, 0 and 5 degrees Celsius respectively. The results showed that with the decreasing temperature, the plant height and root length all showed a downward trend, and the plant height decreased more than root length. The aboveground fresh weight showed an upward trend, while the underground fresh weight showed a downward first and then upward trend. The relative electrical conductivity (REC), tissue water content, total soluble sugar and free proline content all showed an upward trend, while malondialdehyde (MDA) content showed a downward trend in leaves. The content of soluble protein showed a downward first and then upward trend in leaves. The changing trend of roots was the same as leaves in REC, total soluble sugar and soluble protein content, but the REC of roots increased less than leaves. There was no significant change in tissue water content, free proline and MDA content in roots. At the same low temperature, roots were all higher than leaves in the content of total soluble sugar and soluble protein, while the tissue water content and MDA content were the contrary. In conclusion, the cold resistance of the underground part was stronger than aboveground part in Sedum aizoon L. seedling with sprout.
Numerical simulation of polycrystalline aluminum alloy welded joints with micropores
Authors: Du Jiazheng , Nian Xiangchuan , Liu Liya , Deng Wen
Published: November 2019

Aluminum (Al) alloy welded structures are widely used in industry and daily life, while welded joints are easily produced defects, including macro and microscopic defects. It is a hot topic to study the effect of microscopic defects on the properties of welded joints. In this paper, a two-dimensional rate-dependent crystal plasticity model is established by using a numerical model, and then the mechanical behaviors of welded joints with micropores under tensile load is simulated in a mesoscale. The mechanical properties are described by varying the sizes and positions of micropores. The stress-strain response, equivalent plastic strain and equivalent stress distributions of polycrystalline Al alloy welded joints with different micropores are obtained. The results verify that those micropores have certain impact on the start of slip systems and the ability of resisting the deformation of polycrystalline joints. Compared with the positions of micropores, the sizes of micropores have a greater impact on the mechanical properties of the polycrystalline joints.
Research on Adsorption of Cr(VI) in Water by Potassium Chloride Modified Peanut Shell Carbon
Authors: Pan R , Chen Y J , Chen C , Chang H , Ding A F
Published: November 2019

In order to remove Cr (VI) in water effectively, peanut shell carbon was modified by potassium chloride. The results showed that the removal rate of modified peanut shell carbon was 50.3%, increasing by 17.73% compared with peanut shell carbon. The removal and adsorption effects of modified shell carbon on Cr (VI) under different adsorption conditions were investigated and which including pH, adsorbent dosage, adsorption time and the ion concentration. The results showed that the best adsorption removal efficient of Cr (VI) in water can be obtained with pH of 4, adsorption dosage of 0.3g, the adsorption time of 150 min, and the Cr (VI) concentration of 8μg/ml. The removal rate of modified peanut shell carbon reached 99.86% under the condition. The adsorption process fitted the Freundlich adsorption isotherm and conformed to the quasi-second order kinetic equation.
Effects of different selenium concentrations on the stress resistance of grape seedlings
Authors: Liu Lei , Wang Ting , Sui Liyun , Liu Ji , Lin Lijin , Liao Ming’an
Published: November 2019

The effects of different concentrations of selenium (0.00, 0.05, 0.10, 0.25, 0.50, 1.00, 2.00 mg/L) on the stress resistance of grape seedlings were studied by pot experiment. The results showed that the proline content in different parts of grape seedlings showed an increasing trend with the increase of selenium concentration. The activities of SOD, POD and CAT, as well as the relative conductivity, malondialdehyde and soluble protein content of grape seedlings increased first and then decreased with the increase of exogenous selenium concentration. When the concentration of selenium was 0.50 mg/L, the antioxidant enzyme activity and the content of permeate in grape seedlings reached the maximum. In summary, low concentration of selenium can effectively improve the stress resistance of grape seedlings, but when the concentration of selenium is higher than 0.50 mg/L, it has a toxic effect on grape seedlings.
Study on Processing Technology of Low Sugar Rose Jam
Authors: Dan-min Fan
Published: November 2019

Taking fresh rosa rugosa as raw material, the health and nutrient low-sugar rosa rugosa sauce was developed and the processing technology of low-sugar rosa rugosa sauce was studied in this paper, thus providing safe options for the crowd who are not suitable for sugar products. The recipe of low-sugar rosa rugosa sauce and its optimum process were determined via single factor test, orthogonal analysis and sensory evaluation. The test results show that the low-sugar rosa rugosa sauce whose ratio includes 40g fresh rosa rugosa, 0.4g xanthan gum, 2.1g sodium cyclamate and 100g water has obtained the best quality.
Effect of Steam Explosion Modification and in Vitro Simulated Digestion on Antioxidant Capacity of Dietary Fiber of Pineapple Peel
Authors: Liu Mengjie , Jiang Yongchao , Zhang Li , Wang Xiaofang , Lin Lijing
Published: November 2019

Pineapple peel is an important by-product in the processing of pineapple, which is rich in dietary fiber. In this paper, the dietary fiber of pineapple peel was modified by steam explosion method, and its effect on the morphology of dietary fiber was investigated. The dietary fiber was simulated in vitro to investigate its effect on the antioxidant activity of dietary fiber. The results showed that the steam explosion treatment can break the bulk volume of dietary fiber and increase the surface area. The in vitro simulated digestion, especially simulated gastric digestion, improved the FRAP antioxidant capacity of pineapple peel dietary fiber, and the antioxidant activity of dietary fiber modified by steam explosion was higher under the same conditions.
Monitoring of Benggang erosion based on UAV photogrammetry technology
Authors: Liao Kaitao , Song Yuejun , Xie Songhua , Zheng Haijin
Published: November 2019

The benggang is seriously harmful for its tremendous amount of erosion. At present, the traditional methods in monitoring benggang gully are limit to some extent. The UAV photogrammetry technology combined with GIS spatial analysis was adopted to study the benggang in Bailu Village, Gan County, Jiangxi Province, South China. We measured some parameters and comparing the data of benggang at different times. ArcGIS was used to analyse the spatial and temporal distribution characteristics of benggang erosion. The results show that UAV photogrammetry technology has the advantages of high accuracy, high- speed and no damage to the surface ground, it can calculate the point cloud, reconstruct the benggang landform in three dimensions, and generate surface model DEM, which can provides an alternative for the study of benggang erosion monitoring.
Carbon blocks from natural flake graphite and mesophase pitch: fabrication processing, microstructre, mechanical property, and electrical perfromance
Authors: Li Lei , Jiang Chenyang , Huan Yunzhe , Wang Haibin , Zhou Ruixi , Zhong Bo
Published: November 2019

Traditional preparation process of carbon blocks is relatively high-cost and time-cons uming. Hence natural flake graphite (500nm) and mesophase pitch with high charyield were used as filler and binder respectively to solve this problem. In this research, by controlling the molding pressure, the highest temperature of calcination and the content of different additives separately, the properties of the obtained carbon blocks were investigated. SEM,mechanical and electrical tests were carried out to investigate the phase composition and microstructure of the samples. The results showed that the properties of carbon blocks could be improved.
Load forecasting of distribution equipment based on artificial neural network
Authors: Tao Peng , Dai Wan , Miao Zhao , Xu jin Duan , Hengyi Zhou , Siming Peng
Published: November 2019

Distribution network is the key link that affects the level of power supply service. In order to realize first-class modern distribution network and ensure the high quality electric power for residents, it is necessary to change the original working mode of operation and maintenance “passive repair” and the investment mode”treating the symptoms and not the disease” in distribution network. In such a huge distribution network structure, in order to achieve active maintenance and accurate investment construction, load forecasting of distribution network transformers scientifically will play a vital role. This paper proposes a method that realized the reasonable prediction of load in distribution network by using the self-learning and prediction function of artificial neural network and combing with the change characteristics of the load, which has important reference value to guide the construction investment and operation and maintenance of distribution network.
Functional layout of new mode express service center in college campus
Authors: Bai Yan , Fan Lixue
Published: November 2019

As a gathering place for young people, online shopping is very popular and there is a great demand for express delivery. Campus express has become a problem faced by many university logistics departments. It leads to the contradiction between campus security management and express delivery. Based on the investigation of the basic situation of colleges and universities, the paper positions the express service center of colleges and universities. The functions were analyzed. The function of the new mode express service center for college campus includes the mailing area, the pickup area, the free express delivery package area, the student entrance buffer area and the express delivery area.
Research on the Network Construction of Multiple Modes of Transport on Railway Container
Authors: Meng Xiangru
Published: November 2019

Railway container Multiple modes of transport has the advantages of high efficiency, convenient freight transportation, low transportation cost and safe arrival of goods. The network construction of railway container multiple modes of transport makes China’s railway container multiple modes of transport have a certain position in the world. This paper first analyzes the contents of the organization network construction of railway container multiple modes of transport. The infrastructure network on railway container multiple modes of transport is constructed.The information network is constructed from two aspects: container dynamic tracking information and container document transmission information.Finally, the related contents of the operation network construction are put forward.
Research on Countermeasures of Multimodal Transport under “The Belt And Road”
Authors: zhang Jiaxuan , Liu Tianhui , Zhu Yaru , Wang Shuai , Jiang Shaoyong , Hu Zonglei
Published: November 2019

Multimodal transport can integrate the advantages of various types of transportation, improve the efficiency and quality of transportation, meet the requirements of personalized demand for commodity transportation. It is an inevitable trend for production enterprises to cope with the increasingly fierce global competition. Starting from the “The Belt And Road” strategy and the current situation of China’s multimodal transport, this paper analyzes the development of China’s multimodal transport under the “The Belt And Road” strategy. The development of international multimodal transport is analyzed. The countermeasure under“The Belt And Road” is put forward.
Research on Terminal Distribution Model of Urban Logistics
Authors: Han Lu , Zhang Heng , Bi Jiabao , Guo Tianfang , Zhao Peilei , Wang Xu
Published: November 2019

Aiming at improving the efficiency of express terminal distribution,this paper analyses two modes of domestic terminal distribution and establishes a new mode of terminal distribution based on resource integration and redistribution; The problems of terminal distribution are analyzed from six aspects,the process of terminal distribution is simplified, reengineered to improve the quality of service and reduce the cost of express delivery industry. Finally,the Countermeasures of terminal distribution are put forward,which can help express industry to produce intensively,run efficiently and provide high-quality services,and realize the sustainable operation of logistics industry.
The role of the electricity market management committee in the electricity market
Authors: Tang Chenghui , Zhang Fan
Published: November 2019

With the continuous advancement of China’s electricity market construction, the market has increasingly become the main means of resource optimization and allocation. In the construction of the electricity market, government agencies, power grid utilities and other market entities usually have different demands, and there are different opinions in the process of electricity market framework design and rulemaking. The electricity market management committee can serve as an important deliberative entity in the construction of the electricity market. This paper sorts out the current situation of the construction of China’s electricity market management committee, and summarizes and analyses the functions of the electricity market management committee in the major electricity markets all over the world, which can serve as a reference for the construction of the China’s electricity market management committee.
Research on the application of PPP model in smart city projects
Authors: Yang J Q , You J L
Published: November 2019

Compared with traditional infrastructure, where public-private partnerships (PPPs) have been widely used, smart city is a new field for PPP model application. Currently, the PPP model of smart city projects (SCPs) is faced with a tremendous number of problems. In view of this, according to the hierarchy and synergy characteristics of smart city construction, SCPs can be divided into three categories: the single structure, the cross structure, and the overall structure. This paper matches specific PPP operation models for different types of SCPs. Furthermore, the problem tree and target tree analysis methods are adopted to address the problem encountered in the profit-making model, and ultimately the “E-PPP” model is established as an effective solution. In addition, the proposed model can be used as an innovative one to release the constraints of infrastructure and public service supply in smart cities.
Design and implementation of airport vehicle intelligent management and control platform based on 3S
Authors: Cheng Xu , Chen Zongjuan , Sun Erxin , Shan Zemeng , Zang Dandan , Li Xiaoxiao
Published: November 2019

At present, there exists the phenomenon that each airport management department does things in his own way and each system operates independently, so it is necessary to establish a unified platform to realize the collaborative operation of multiple departments. In this paper, 3S (GIS/GPS/RS) technology is used to realize precise positioning of vehicles, personnel and flights through multi-sensor fusion. To achieve the dynamic monitoring of vehicles, automatic alarm and fence management, draw a custom electronic map of the airport based on the high-resolution remote sensing image. Adaptive genetic algorithm is applied to optimizing vehicle scheduling. The vehicle asset analysis and vehicle maintenance management are integrated into the vehicle intelligent control platform, so as to realize the organic integration of airlines, ground service, maintenance center, flight, asset procurement and multiple departments of various organizations. Through the establishment of the platform, the accurate and reasonable dispatching and operation of ground vehicles can be achieved, the operational efficiency of the airport can be effectively improved, and the maintenance of airport ground safety provides a powerful guarantee.
Identification of Endophytic and Rhizosphere Bacteria in Maize (Zea mays L.) in Limapuluh Kota Region, West Sumatra, Indonesia
Authors: Sondang Y , Anty K , Siregar R
Published: November 2019

The variety of microorganisms that are very abundant began to be used for the benefit of sustainable agriculture. A group of non-pathogenic bacteria has been reported to control disease-causing pathogens and improve plant growth. The purpose of this study is (a) to identify and characterize endophytic bacteria and corn rhizosphere (b) to study endophytic bacteria and rhizosphere which have the potential to spur plant growth. The experiment was carried out at the Payakumbuh State of Agricultural Polytechnic Laboratory, Limapuluh Kota Region, West Sumatra, and LIPI Bogor. The sampling method was carried out by random sampling at four locations. Endophytic bacteria taken from the roots of maize and rhizosphere bacteria originated from the soil around the roots. Bacterial isolation was carried out by the pour and the streak plate method. Identification of bacterial characteristics based on the form of colonies, edges, surfaces, rough surface roughness, surface color, pigment color, bacterial body density, and halo zone colonies. Six species of bacteria have been identified using molecular analysis based on 16S rRNA gene fragments. Bacteria are dominated by the Bacillus genera compared to the Pseudomonas genera. The ability of phosphate dissolving bacteria indicates that endophytic bacteria and the rhizosphere act as growth stimulants.
Phosphorus Uptakes and Yields of Sweet Corn Grown under Organic Production System
Authors: Fahrurrozi Fahrurrozi , Muktamar Zainal , Sudjatmiko Sigit , Chozin Mohammad , Setyowati Nanik
Published: November 2019

Organic production system for sweet corn applied both solid and liquid organic fertilizer. The use liquid organic fertilizer potentially changes the availability of phosphorus for organically grown sweet-corn. Phosphorus absorption in sweet corn production determines the quality and quality of crop yields. This study aimed to determine the relationship between phosphorus uptakes and yields of sweet corn. A field experiment was arranged in randomized complete block design with three replications. Twenty sweet corn varieties were grown under organic environment where soil was fertilized with 30 tons ha-1 of cattle-based vermicompost. At 14, 21, 28 and 35 days after planting, each plant was additionally sprayed with thitonia-enriched liquid organic fertilizer of 50, 100, 200, 300 ml plant-1. Observations were conducted on phosphorus uptakes by plant, leaf phosphorus content, shoot dry weight per plot, days to harvesting, weight of husked ear, and weight of unhusked ear as well as sweet corn yield per plot. Results indicated that phosphorus uptakes by sweet corn significantly increased leaf phosphorus content, (r=0.56), shoot dry weight per plot (r=0.83), weight of husked ear (r=0.60), weight of unhusked ear (r=0.56) and sweet corn yield per plot (r=0.70). Phosphorus uptakes by sweet corn also significantly decreased days to harvesting (r=-0.27). In conclusion, phosphorus uptakes determined growth and yields sweet corn grown under fully organic environment.
Viability Spermatozoa Epididymis of Buffalo (Bubalus Bubalis) In Fertilized Media to Additional Serum at Temperature 5°C
Authors: Harissatria  , Surtina D. , Astuti T. , Jaswandi  , Hendri
Published: November 2019

This study purposed to determine the quality of Buffalo’s epididymis Spermatozoa on the TALP (Tyrode Albumin Sodium Lactate Sodium Pyruvate) fertilization at the 5°C temperature. The Randomized Block Design used on this research with 3 treatments and 5 blocks as replication. The treatments were additional serum: P1 (0%), P2 (10%) and P3 (20%). The results showed that the highest motility was on the treatment P3 (20%), about 66.00±9.45 was significantly effect (P<0.05) than P1 and P2. The highest percentage of live on P3 (20%) was 75.20±2.41 and significantly effect (P<0.05) than P1 and P2. The best percentage of abnormality was also on the P3 (20%) i.e.14.20±1.30 significantly effect (P<0.05) compare P1 and P2. It could be concluded that the addition of 20% serum in TALP media as a fertilization medium can improve the buffalo’s spermatozoa quality of caudal epididymis.
The Effect of Using Some Kind of Manure on the Content of Dry Matter, Organic Matter and Crude Protein of Elephant Grass Cv. Taiwan (Pennisetum purpureum) on the First Harvest
Authors: Surtina D. , Sari R.M. , Astuti T. , Kusuma A.H.
Published: November 2019

This research aimed to determine the effect of different types of manure on dry matter content, organic matter and crude protein of elephant grass (Pennisetum purpureum) cv. Taiwan on the first harvest. This study used a completely randomized design, with 4 treatments and 4 replications for each treatment. The treatment were P1 = 100% acid soil (without manure/control), P2 = 80% acid soil + 20% cow manure, P3 = 80% acid soil + 20% goat manure, P4 = 80% acid soil + 20% of poultry manure. The best results on this researched obtained the highest dry matter content of elephant grass was P2 (16.07), organic matter on P2 (86.36), and the highest crude protein was P3 (14.12). It can be concluded that the using of different manure on the elephant grass (Pennisetum purpureum) cv. Taiwan has a highly significant effect (P <0.01) on dry matter content, and has a significant effect (P <0.05) on the content of organic matter and crude protein of elephant grass (Pennisetum purpureum) cv. Taiwan at the first harvest.
Moringa Seeds (Moringa olifiera L.) Application as Natural Coagulant in Coffee Wastewater Treatment
Authors: Novita Elida , Wahyuningsih Sri , Pradana Hendra Andiananta , Marsut Wendy Dreifyana , Farisul F Akhmad
Published: November 2019

The coffee processing will produce a wastewater with a low pH. It is corrosive and dangerous for the environment. One of the alternative solution to solve this problem is by applying the coagulation-flocculation treatment. The natural coagulant that can be applied is the moringa seeds powder. The aim of this research is to optimize the coagulation – flocculation treatment by using mesh variation moringa seed powder in coffee wastewater pollution decreasing. Experimental design used a completely randomized design (CRD) to dosage the optimization based on mesh size variation in moringa seed powder with rind and rindless toward coffee wastewater pollution decreasing. Duncan assay showed that with α < 0.05, mesh size variation in moringa seed powder with rind and rindless was significantly different. The best treatment in coagulation – flocculation using moringa seed as nature coagulant was rindless moringa seed powder. The optimal particle size was 60 mesh due for having a little efficiency level with 70 mesh and 80 mesh and it is easier than the manual one. It has the efficiency level decreasing in TSS, turbidity and COD of 60 mesh was 69.44 ± 1%; 88.15 ± 0.5 % and 41.80 ± 0.5 %.
Challenges in Molecular Identification of Endomycorrhizal Fungi from Rhizosphere of Cashew Plant
Authors: Pharmawati Made , Proborini Meitini Wahyuni
Published: November 2019

Molecular identification of endomycorrhiza through DNA analysis is needed to complement identification based on morphological characters. This research aimed to study molecular DNA techniques to identify endomycorrhiza in the rhizosphere of cashew plants (Anacardium occidentale L.). DNA extraction was carried out using CTAB buffer from cashew roots, soil and spores and PCR was carried out with specific primers for mycorrhiza. The primers used were the primer pairs of SSU-Glom1 and NDL22 or LR4 + 2 and SSUmCf-LSUmBr. The results showed that DNA extracted from endomycorrhizal spores has the potential for molecular identification of endomycorrhiza, however it needs high amount of spores to obtain high quality and quantity DNA. DNA samples from cashew roots and cashew rhizosphere soil showed no PCR product and could not be used to identify endomycorrhiza molecularly. Optimization of PCR conditions and primer selection are needed to obtain better PCR products.
Isolation and Identification of Lactic Acid Bacteria Producing Biopreservative Bacteriosin from Smoked Fish
Authors: Rialita Tita , Sukarminah Een , Yuliana Try , Sumanti Debby Moody , Kurnianingrium Intan , Octaviani Fanny Nur , Santoso Marcellia Bella , Susanto Heditia Febby
Published: November 2019

Bacteriocin was a product of bacterial metabolites that have antimicrobial properties, so that it had the potential to be developed into biopreservation material in the preservation of food products. Bacteriocin was produced by lactic acid bacteria (LAB), one of the sources of which was from smoked fish products. Some regions in Indonesia produce various types of smoked fish from various types of fish, which were thought to contain bacteriocin-producing lactic acid bacteria. This study aims to isolate and identify BAL from several types of smoked fish, and to determine the antimicrobial activity of LAB isolate as candidate biopreservative to food product relative to fish product. The research method used was explorative which was analysed descriptively. The results showed that several LAB isolates from various types of smoked fish could potentially produce bacteriocin which can inhibit pathogen bacteria with a broad inhibitory spectrum.
Influence of Liquid Smoke Cinnamon Against Attacks Leaf Rot Disease (Phytophthora Infestans) on Potato (Solanum Tuberosum L.)
Authors: Budaraga I Ketut , Tukiran  , Syamsuwirman
Published: November 2019

Shaped research on the effect of using liquid smoke cinnamon against late blight (Phytophthora infestans) on potato (Solanum tuberasum L.) has been conducted in the village of Gunung Tujuh Pelompek District of Kerinci regency in Jambi Province. The purpose of this study was to determine the effect of the use of liquid smoke against late blight (Phytophthora infestans) on potato and other effects related to growth factors and production of potato (Solanum tuberasum L). The experiments were conducted in the field using a randomized block design with 8 treatments and 3 replications thus obtained 24 experimental unit. Each experimental unit consisted of 35 plants. As the plant sample under observation for variable growth is as much as 5 samples and disease variables is the entire plant for each plot. The treatments were the use of multiple doses of liquid smoke for spraying are: A = non-treated liquid smoke (WAPer only), B = fungicide interval of 7 days, C = treated liquid smoke 25 cc/liter of WAPer alternating with fungicides, D = treated liquid smoke 50 cc/liter of WAPer alternating with fungicides, E = treatment liquid smoke 75 cc/liter of WAPer hose alternating with fungicides, F = treatment liquid smoke 100 cc/liter of WAPer alternating with fungicides, G = liquid smoke treatment of 125 cc/liter of WAPer alternating with fungicides, and H = treatment liquid smoke 150 cc/liter of WAPer. The data were analyzed statistically by F test if the F-test has a significantly different effect and then continued with Duncan’s New Multiple Range Test (DNMRT) at the 5% significance level. The results show that the use of different doses of liquid smoke influence of highly significant observation parameters, namely: plant height, length, and width of leaves, leaf number, and weight of tubers per plant, number of tubers per plant, tuber weight per plot, intensity rot disease and the percentage of leaf blight attack. Plants treated with the liquid smoke with a rate of 125 cc punctuated alternately with fungicides showed intensity and percentage of the lowest disease. It is advisable to do further studies with different doses and with no fungicide treatment on the season and a different location.
Spermatozoa Quality of Young Male White Rat after Treated with Moringa oleifera Leaf Extract
Authors: Suaskara I B M , Joni M , Setyawati I
Published: November 2019

Spermatogenesis is the process of spermatozoa formation from primitive germinativum cells. There are Leydig (interstitial) cells outside the seminiferous tubules that secrete testosterone hormone into the blood. The quality of spermatozoa is largely determined by the nutrients consumed during the growth period. Moringa leaves contain lots of nutrients, therefore it is often used to improve malnutrition in children. The specific purpose of this study was to determine the quality of spermatozoa of young male rats after treated with Moringa leaf extracts. This study used a randomized complete block design (RCBD) and divided into 4 groups of treatment consisted of 10 young male white rats i.e. control group (K0), treatment group 1 (K1) treated with dose 50 mg/kg, group 2 (K2) treated with dose 75 mg/kg, group 3 (K3) treated with dose 100 mg/kg of Moringa leaf extracts. Parameters observed were sperm quality (sperm count, motility, viability and morphology). The result of this research showed that the treatment of Moringa leaf extracts in the growth period could increase spermatozoa quality at dose 50 and 75 mg/kg body weight (bw significantly. Moringa leaf extracts at dose 100 mg/kg body weight (bw) could decrease spermatozoa quality significantly (p<0.005). The result proved that the higher doses of Moringa leaf extracts given to young male rats could decrease the quality of spermatozoa.
Antifungal Activities Of Cinnamon Leaf Extracts Against Sigatoka Fungus (Pseudocercospora Fijiensis)
Authors: Darmadi Anak Agung Ketut , Sudirga Sang Ketut , Suriani Ni Luh , Wahyuni I Gusti Ayu Sugi
Published: November 2019

Efforts to reduce the negative impacts caused by the use of synthetic fungicides need to be done in implementing environmentally sustainable agriculture. The use of biofungicides should be done. The acetone extract of cinnamon leaves has the potential as a biofungicide. Based on this, a series of experiments were carried out: crude cinnamon extract test against the test fungus, i.e. the fungus causing Sigatoka disease in banana plants, MIC (Minimum Inhibiting Concntration) extract, effectiveness test of extract on colony, biomass, spore formation, and identification of cinnamon leaf extract content based on partition, column chromatography and GS-MS analysis. The rough extract of cinnamon leaves inhibited the growth of the Sigatoka fungi (P. fijiensis) in-vitro on PDA media with a diameter of 30 mm which means that this extract has a very strong inhibitory activity, with a MIC value of 0.5% (w/v). Treatment of extracts with concentrations between 0.1 and 0.5% significantly (P <0.05) inhibited the growth of fungal colonies, fungal biomass and fungal spore formation. There were 16 compounds found in the active fraction of cinnamon leaf extracts and 3 dominant compounds that are anti-fungal compounds, namely 1.2-Benzenedicarboxylic acid, mono 2-ethyl (29%), 2H-I-Benzopyran-2- one (CAS) Coumarin (11.9%) and 2,6-Dimethyl-6-nitro-2-hepten-4-one (11.5%).
Bioactive compound of Streptomyces capoamus as biocontrol of Bacterial Wilt Disease on Banana Plant
Authors: Kawuri R , Darmayasa I B G
Published: November 2019

Banana is a plant that has a high economic value for human to fulfil his daily fruits need.. The antagonistic test between nine isolates Streptomyces sp. and R. solanacearum showed that the Streptomyces sp. 9 has the highest resistance (19 mm), indicating a strong inhibition and has smallest concentrations of inhibition (MIC) of 10%. The glasshouse scale test demonstrated that the Streptomyces sp. 9 culture sprayed 4 times on a different day could suppress bacterial wilt disease by 100% from 11% in control. The research was to determine molecular identification of Streptomyces sp.9 using 16S rRNA and identification of active compound by Gass Chromatography Mass Spectra (GCMS). The results showed that the molecular identified Streptomyces sp.9 as a Streptomyces capoamus. The chemical compounds which have antimicrobial are Hexadeconoic acid methyl ester, Heneicosane, Hexacosane(CAS) n-Hexacosane, Limonene, Dotriacontane and Tetracontane as antimicrobial agent. The conclusion was Streptomyces capoamus showed various bioactive compounds, which is importance as potential biocontrol on bacterial wilt disease on banana plants. There could be probably of new bioactive compounds and this also provided a new insight towards the development of good candidates for bioactive natural products.
Application of coconut biochar and organic materials to improve soil environmental
Authors: Herlambang Susila , S AZ.Purwono Budi , Sutiono Heru Tri , Rina N Susanti
Published: November 2019

Inceptisol is a land which suitable for brick making, where the industry will take several meters of topsoil for raw material of brick industry. The loss of topsoil causes soil damage, while the topsoil is a source of nutrition for plant growth. The purpose of this study is to improve the soil environment because damage is caused by the brick industry. The application of the material used is biochar from coconut shell and cow manure with a dose of 0 tons per hectare, 10 tons per hectare, 15 tons per hectare, and 20 tons per hectare which is incubated in the topsoil for 0 months, 1 month, 2 month and 3 months. The best content of organic matter in the soil environment is around 2%. If it is lacking, then there must be added ingredients that contain enough C-organic and nutrients for good soil environmental conditions. The treatment of biochar and organic matter can improve the soil environment because it is a source of C and nutrients. Both of these materials function improve the physical and chemical properties of the soil such as cation exchange capacity (CEC) and soil pH. Changing characteristics will create a better soil environment so that soil nutrients will become more available for growth.
Application Nano Zeolite-Molybdate For Avocado Ripeness Indicator
Authors: Putri VJ , Warsiki E , Syamsu K , Iskandar A
Published: November 2019

Zeolite is aluminosilicate containing pores and cavities that are usually used as absorbent materials. Nano zeolite - molybdate was used as a ripeness indicator of avocado. The ripeness indicator was made by dissolving molybdate in hydrogen peroxide (H2O2) and mixed with nano zeolite. This study was aimed to determine the colour change of indicator, and the relationship between the changing colour of indicator and the ripening of avocado. The ripeness indicator was prepared used activated nano zeolite with HCl 1 N for 24 h, then mixed with 4 mL peroxomolybdate. Nano zeolite-molybdate tablets was packed using 0,04 mm thick of LDPE plastic, and it was inserted inside avocado packaging. The ripeness indicator would identified by the ethylene production of avocados, and resulted changing the indicator colour from yellow to blue. The yellow colour would represent unripe avocado, but the blue colour would represent ripe avocados. The colour changing was related with the decrease of avocado quality during 10 days storage with the rate of weight loss 0.0113%, the rate of sugar level 0.7182% and the rate of avocado hardness 2.1132% by day.
Gambir Quality from West Sumatra Indonesia Processed With Traditional Extraction
Authors: Anggraini Tuty , Neswati  , Asben Alfi
Published: November 2019

Gambir is dried extract of leaves and branches of Uncaria gambir. Pesisir Selatan and Lima Puluh Kota were the central place in produce gambir in West Sumatra, Indonesia. This study identified gambir from both Pesisir Selatan and Lima Puluh Kota West Sumatra Indonesia for its quality. The analysis of gambir were the antioxidant activity, total polyphenol content and catechin content. Catechin content determined with High Performance Liquid Chromatography (HPLC). The result showed that the antioxidant activity, total polyphenols and the average of catechin content gambir from Pesisir Selatan and Lima Puluh Kota Region were 91.33 % and 91.55 %, 197.35 mg/m GAE and 182.00 mg/g GAE and 50.87 % and 55.40% respectively. The conclusion were the antioxidant activity, total polyphenols and catechin content of gambir from Pesisir Selatan and Lima Puluh Kota are influenced by the raw materials.
Isolation and identification of indigenous lactic acid bacteria on corn flour BISI-16 during spontaneous fermentation process
Authors: Sukainah Andi , Johannes Eva , Fadilah Ratnawaty , Mustarin Amirah , Putra ReskiPraja
Published: November 2019

Lactic acid bacteria (LAB) is one of the dominant bacteria species that have an important role during spontaneous fermentation process of corn flour BISI-16. LAB are known to change the chemical structure of natural corn starch through the activity of enzyme and lactic acid produced during fermentation process. This change of structure causes the modification of physicochemical properties of corn flour. The objectives of this study were to isolate and identify indigenous LAB involved in spontaneous fermentation process of BISI-16 cornflakes. Isolation and identification of early LAB was done by isolation morphology characterization including cell shape, Gram staining, catalase test, and endospora staining. Furthermore, isolates LAB were identified by their genotypes using Polymerase Chain Reaction (PCR) method and 16S rRNA sequencing analysis. The results showed two isolates (ASN3 and ASN5) that had been isolated from corn flour during spontaneous fermentation and were grown on MRSA media having rounded colonic and creamy, rod-shaped cells, Gram (+), catalase (-), and endospores (-). The initial identification stage shows both isolates as candidate LAB. The results of the 16S rRNA sequence analysis showed both isolates were genotypically similar with Lactobacillus fabifermentans with 97% similarity for ASN3 isolates and ASN5 isolates of 98%.
The Influence of Lactic Acid Bacteria Lactobasillus Casei For performance of Broiler
Authors: Mega Sari R , Akbar Syahro Ali
Published: November 2019

This research aims to determine the influence of addition of probiotic lactic acid bacteria Lactobasillus casei which derived from Yakult toward the performance of broiler chicken. This research used complete randomized design with four treatments and two replications. This research used 60 DOC CP 707 from Charoen Pokhpand Indonesia, Yakult as the source of baceria Lactobasillus casei, commercial feed 511 and 311, and drinking water. If the F count treatment is higher than F table of 5 % meal, so it would continued with Duncan test. This research was carried out with direct observation and weighing in the experimental field. Based on the result of the research it can be seen that the addition of lactic acid bacteria Lactobasillus casei had a very significant effect (P <0.01) on body weight gain and feed conversion and had no significant effect (P> 0.05) on the consumption of broiler chicken feed. Based on the results of the research it can be concluded that the addition of lactic acid bacteria Lactobasillus caseihas an effect on body weight gain and feed conversion. Where the lactobasillus casei lactic acid bacteria doses 1.5 ml helps digestion and absorption of fed so it can increase the body weight gain with the best feed conversion.
Metabolite profiling of Indonesian cacao using Gas Chromatography-Mass Spectrometry
Authors: Devy Lukita , Susilo Agung Wahyu , Wachjar Ade , Sobir
Published: November 2019

Indonesia as the world third largest cacao producer is also well known for its fine cacao from Trinitario group. Fine cacao breeding will support the sustainability of Indonesian specialty cocoa products. However, information on the distinction between metabolite profile of Indonesian fine and bulk cacao are still limited. The objective of this study was to compare the metabolite profile of Indonesian fine and bulk cacaos using Gas Chromatography-Mass Spectrometry (GC-MS) method with methanol-formic acid-water extraction. Four Indonesian fine cacaos (i.e. DR 2, DRC 16, PNT 16, PNT 17) and bulk cacaos (i.e. MCC 02, KW 516, KW 617, SUL 01) were used. Measurements were conducted on untargeted metabolite profile, theobromine (T), caffeine (C), T/C and total fat content. Forty-nine peaks were detected and 23 putative metabolites with high reliability were identified. Principal component analysis separated fine and bulk cacaos into two different groups. Hierarchical cluster analysis showed fine cacaos contained higher caffeine while bulk cacaos contained higher fatty acids (palmitic acid, stearic acid and oleic acid) content than the opposite. However only fatty acids, which represented in total fat content, showed stable result under targeted analysis. Therefore, these metabolites could be further analysed as biomarker for fine cacao selection.