bluuebunny/crossref_metadata_2025 huggingface dataset:
Which Sugar to Take and How Much to Take? Two Distinct Decisions Mediated by Separate Sensory Channels
Authors: Kohatsu Soh , Tanabe Noriko , Yamamoto Daisuke , Isono Kunio
Published: June 2022

In Drosophila melanogaster, gustatory receptor neurons (GRNs) for sugar taste coexpress various combinations of gustatory receptor (Gr) genes and are found in multiple sites in the body. To determine whether diverse sugar GRNs expressing different combinations of Grs have distinct behavioral roles, we examined the effects on feeding behavior of genetic manipulations which promote or suppress functions of GRNs that express either or both of the sugar receptor genesGr5a (Gr5a+ GRNs) and Gr61a (Gr61a+ GRNs). Cell-population-specific overexpression of the wild-type form of Gr5a (Gr5a+) in the Gr5a mutant background revealed that Gr61a+ GRNs localized on the legs and internal mouthpart critically contribute to food choice but not to meal size decisions, while Gr5a+ GRNs, which are broadly expressed in many sugar-responsive cells across the body with an enrichment in the labella, are involved in both food choice and meal size decisions. The legs harbor two classes of Gr61a expressing GRNs, one with Gr5a expression (Gr5a+/Gr61a+ GRNs) and the other without Gr5aexpression (Gr5a−/Gr61a+ GRNs). We found that blocking the Gr5a+ class in the entire body reduced the preference for trehalose and blocking the Gr5a- class reduced the preference for fructose. These two subsets of GRNsare also different in their central projections: axons of tarsal Gr5a+/Gr61a+ GRNs terminate exclusively in the ventral nerve cord, while some axons of tarsal Gr5a−/Gr61a+ GRNs ascend through the cervical connectives to terminate in the subesophageal ganglion. We propose that tarsal Gr5a+/Gr61a+ GRNs and Gr5a−/Gr61a+ GRNs represent functionally distinct sensory pathways that function differently in food preference and meal-size decisions.
Exploring the Role of Posttranslational Modifications in Spinal and Bulbar Muscular Atrophy
Authors: Gogia Neha , Ni Luhan , Olmos Victor , Haidery Fatema , Luttik Kimberly , Lim Janghoo
Published: June 2022

Spinal and Bulbar Muscular Atrophy (SBMA) is an X-linked adult-onset progressive neuromuscular disease that affects the spinal and bulbar motor neurons and skeletal muscles. SBMA is caused by expansion of polymorphic CAG trinucleotide repeats in the Androgen Receptor (AR) gene, resulting in expanded glutamine tract in the AR protein. Polyglutamine (polyQ) expansion renders the mutant AR protein toxic, resulting in the formation of mutant protein aggregates and cell death. This classifies SBMA as one of the nine known polyQ diseases. Like other polyQ disorders, the expansion of the polyQ tract in the AR protein is the main genetic cause of the disease; however, multiple other mechanisms besides the polyQ tract expansion also contribute to the SBMA disease pathophysiology. Posttranslational modifications (PTMs), including phosphorylation, acetylation, methylation, ubiquitination, and SUMOylation are a category of mechanisms by which the functionality of AR has been found to be significantly modulated and can alter the neurotoxicity of SBMA. This review summarizes the different PTMs and their effects in regulating the AR function and discusses their pathogenic or protective roles in context of SBMA. This review also includes the therapeutic approaches that target the PTMs of AR in an effort to reduce the mutant AR-mediated toxicity in SBMA.
SHANK3 Antibody Validation: Differential Performance in Western Blotting, Immunocyto- and Immunohistochemistry
Authors: Lutz Anne-Kathrin , Bauer Helen Friedericke , Ioannidis Valentin , Schön Michael , Boeckers Tobias M.
Published: June 2022

SHANK3 is a scaffolding protein implicated in autism spectrum disorders (ASD). Its function at excitatory glutamatergic synapses has been studied for the last two decades, however, tissue-specific expression patterns as well as its subcellular localization need to be studied in further detail. Especially the close sequence homology of SHANK3 to its protein family members SHANK2 and SHANK1 raises the emerging need for specific antibodies that are validated for the desired methodology. With this study, we aim to validate a set of commercial as well as homemade SHANK3 antibodies in Western Blotting, and synaptic immunocyto- and immunohistochemistry. We found that only a small subset of the antibodies included in this study meet the criteria of quality and specificity. Therefore, we aim to share our findings on SHANK3 antibody validation but also raise awareness of the necessity of antibody specificity testing in the field.
A Radiomics Model for Preoperative Predicting Sentinel Lymph Node Metastasis in Breast Cancer Based on Dynamic Contrast-Enhanced MRI
Authors: Ma Mingming , Jiang Yuan , Qin Naishan , Zhang Xiaodong , Zhang Yaofeng , Wang Xiangpeng , Wang Xiaoying
Published: June 2022

Purpose

To develop a radiomics model based on preoperative dynamic contrast-enhanced MRI (DCE-MRI) to identify sentinel lymph node (SLN) metastasis in breast cancer (BC) patients.

Materials and Methods

The MRI images and clinicopathological data of 142 female primary BC patients from January 2017 to December 2018 were included in this study. The patients were randomly divided into the training and testing cohorts at a ratio of 7:3. Four types of radiomics models were built: 1) a radiomics model based on the region of interest (ROI) of breast tumor; 2) a radiomics model based on the ROI of intra- and peri-breast tumor; 3) a radiomics model based on the ROI of axillary lymph node (ALN); 4) a radiomics model based on the ROI of ALN and breast tumor. Receiver operating characteristic (ROC) curve analysis and decision curve analysis (DCA) were used to assess the performance of the three radiomics models. The technique for order of preference by similarity to ideal solution (TOPSIS) through decision matrix analysis was used to select the best model.

Results

Models 1, 2, 3, and 4 yielded AUCs of 0.977, 0.999, 0.882, and 1.000 in the training set and 0.699, 0.817, 0.906, and 0.696 in the testing set, respectively, in terms of predicting SLN metastasis. Model 3 had the highest AUC in the testing cohort, and only the difference from Model 1 was statistically significant (p = 0.022). DCA showed that Model 3 yielded a greater net benefit to predict SLN metastasis than the other three models in the testing cohort. The best model analyzed by TOPSIS was Model 3, and the method’s names for normalization, dimensionality reduction, feature selection, and classification are mean, principal component analysis (PCA), ANOVA, and support vector machine (SVM), respectively.

Conclusion

ALN radiomics feature extraction on DCE-MRI is a potential method to evaluate SLN status in BC patients.
Plasma Metabolites Forecast Occurrence and Prognosis for Patients With Diffuse Large B-Cell Lymphoma
Authors: Fei Fei , Zheng Meihong , Xu Zhenzhen , Sun Runbin , Chen Xin , Cao Bei , Li Juan
Published: June 2022

Background

Diffuse large B-cell lymphoma (DLBCL) is the most common non-Hodgkin’s lymphoma with considerable heterogeneity and different clinical prognosis. However, plasma metabomics used to forecast occurrence and prognosis of DLBCL are rarely addressed.

Method

A total of 65 volunteers including 22 healthy controls (Ctrl), 25 DLBCL patients newly diagnosed (ND), and 18 DLBCL patients achieving complete remission (CR) were enrolled. A gas chromatography mass spectrometry-based untargeted plasma metabolomics analysis was performed.

Results

Multivariate statistical analysis displayed distinct metabolic features among Crtl, ND, and CR groups. Surprisingly, metabolic profiles of newly diagnosed DLBCL patients undergoing different prognosis showed clear and distinctive clustering. Based on the candidate metabolic biomarkers (glucose and aspartate) and clinical indicators (lymphocyte, red blood count, and hemoglobin), a distinct diagnostic equation was established showing improved diagnostic performance with an area under curve of 0.936. The enrichment of citric acid cycle, deficiency of branched chain amino acid, methionine, and cysteine in newly diagnosed DLBCL patients was closely associated with poor prognosis. In addition, we found that malate and 2-hydroxy-2-methylbutyric acid were positively correlated with the baseline tumor metabolic parameters (metabolically active tumor volume and total lesion glycolysis), and the higher abundance of plasma malate, the poorer survival.

Conclusion

Our preliminary data suggested plasma metabolomics study was informative to characterize the metabolic phenotypes and forecast occurrence and prognosis of DLBCL. Malate was identified as an unfavorable metabolic biomarker for prognosis-prediction of DLBCL, which provided a new insight on risk-stratification and therapeutic targets of DLBCL. More studies to confirm these associations and investigate potential mechanisms are in the process.
Lung Function in Preschool Children in Low and Middle Income Countries: An Under-Represented Potential Tool to Strengthen Child Health
Authors: Chaya Shaakira , Zar Heather J. , Gray Diane M.
Published: June 2022

Background

The burden of respiratory disease is high in low-middle income countries (LMIC). Pulmonary function tests are useful as an objective measure of lung health and to track progression. Spirometry is the commonest test, but its use is limited in preschool children. Other lung function methods have been developed but their use in LMIC has not been well described.

Aim

To review the use of preschool lung function testing in children in LMIC, with particular reference to feasibility and clinical applications.

Methods

Electronic databases “PubMed”, “Scopus”,” Web of Science”, and “EBSCO host” were searched for publications in low and middle income countries on preschool lung function testing, including spirometry, fractional exhaled nitric oxide (FeNO), oscillometry, interrupter technique, tidal breathing and multiple breath washout (MBW), from 1 January 2011 to 31 January 2022. Papers in English were included and those including only children ≥6 years were excluded.

Result

A total of 61 papers from LMIC in Asia, South America, Africa, Eurasia or the Middle East were included. Of these, 40 included spirometry, 7 FeNO, 15 oscillometry, 2 interrupter technique, and 2 tidal breathing. The papers covered test feasibility (19/61), clinical application (46/61) or epidemiological studies (13/61). Lung function testing was successful in preschool children from LMIC. Spirometry was the most technically demanding and success gradually increased with age.

Conclusion

Preschool lung function testing is under-represented in LMIC for the burden of respiratory disease. These tests have the potential to strengthen respiratory care in LMIC, however access needs to be improved.
Clinical Effectiveness of Conservative Treatments on Lumbar Spinal Stenosis: A Network Meta-Analysis
Authors: Chen Xuanwei , Zheng Zhizhan , Lin Jianhua
Published: June 2022

Objective: To systematically evaluate the clinical effectiveness of conservative treatments including pharmacological treatments and nonpharmacological treatments on patients with lumbar spinal stenosis.

Methods: We searched six electronic databases systematically for randomized clinical trials published between January 2000 and July 2021, including the China National Knowledge Infrastructure, WanFang Data, PubMed, MEDLINE, Embase, and the Cochrane library. The studies focused on the therapeutic effects of pharmacological treatments including calcitonin, antiepileptics, neurotrophic drugs, nonsteroidal anti-inflammatory drugs, Chinese Traditional Medicine, limaprost, and nonpharmacological treatments like physiotherapy for treating lumbar spinal stenosis were included. The outcome was measured using the visual analog scale, Oswestry Dysfunction Index, Japanese Orthopaedic Association Score, and EuroQol Five Dimensions Questionnaire. The quality of eligible studies was assessed by using the Cochrane recommended bias risk assessment tool. Stata was used to conduct the network meta-analysis.

Results: A total of 12 randomized control trials with 1,194 patients were included. The network meta-analysis showed that for the visual analog scale, a better therapeutic effect was noted while using Chinese Traditional Medicine and physiotherapy, followed by analgesics drugs and limaprost. Limaprost and calcitonin were better in decreasing the Oswestry Dysfunction Index. In terms of the Japanese Orthopaedic Association Score, the use of traditional Chinese Medicine and limaprost were associated with a better improvement than other treatments. Meanwhile, limaprost combined with analgesics drugs was found to be effective to improve the EuroQol Five Dimensions Questionnaire.

Conclusion: Among the commonly used conservative treatments for the treatment of lumbar spinal stenosis, limaprost may have better efficacy in improving the Japanese Orthopaedic Association Score and decreasing the Oswestry Dysfunction Index, with a beneficial effect on decreasing the visual analog scale and improving the EuroQol Five Dimensions Questionnaire.

Systematic Review Registration: website, identifier registration number.
Vitamin D: A Potential Star for Treating Chronic Pancreatitis
Authors: Zheng Meifang , Gao Runping
Published: June 2022

Chronic pancreatitis (CP) is a chronic inflammatory and fibrotic disease of the pancreas. The incidence of CP is increasing worldwide but the effective therapies are lacking. Hence, it is necessary to identify economical and effective agents for the treatment of CP patients. Vitamin D (VD) and its analogues have been confirmed as pleiotropic regulators of cell proliferation, apoptosis, differentiation and autophagy. Clinical studies show that VD deficiency is prevalent in CP patients. However, the correlation between VD level and the risk of CP remains controversial. VD and its analogues have been demonstrated to inhibit pancreatic fibrosis by suppressing the activation of pancreatic stellate cells and the production of extracellular matrix. Limited clinical trials have shown that the supplement of VD can improve VD deficiency in patients with CP, suggesting a potential therapeutic value of VD in CP. However, the mechanisms by which VD and its analogues inhibit pancreatic fibrosis have not been fully elucidated. We are reviewing the current literature concerning the risk factors for developing CP, prevalence of VD deficiency in CP, mechanisms of VD action in PSC-mediated fibrogenesis during the development of CP and potential therapeutic applications of VD and its analogues in the treatment of CP.
Mitochonic Acid-5 Inhibits Reactive Oxygen Species Production and Improves Human Chondrocyte Survival by Upregulating SIRT3-Mediated, Parkin-dependent Mitophagy
Authors: Xin Ruobing , Xu Yiyang , Long Dianbo , Mao Guping , Liao Hongyi , Zhang Ziji , Kang Yan
Published: June 2022

Mitochondrial dysfunction is related to the pathogenesis of osteoarthritis (OA); however, there are no effective drugs to treat OA for maintaining mitochondrial homeostasis. Studies have shown that mitochonic acid-5 (MA-5) has a protective effect against mitochondrial damage and plays a role in mitophagy. However, it is not clear whether MA-5 has a beneficial effect on inflammatory articular cartilage. Here, human OA cartilage was obtained from patients undergoing total joint replacement. Interleukin-1β (IL-1β) was used to stimulate chondrocytes and induce inflammatory injury. Cell Counting Kit-8, TUNEL, and flow cytometry assays were used to assess apoptosis. Gene expression was examined using quantitative reverse transcription-polymerase chain reaction. Mitochondrial function was evaluated using immunoblotting, mitochondrial membrane potential assay, JC-1 staining, and immunofluorescence analysis. Mitophagy was detected using immunoblotting and immunofluorescence. 3-(1H-1,2,3-triazol-4-yl) pyridine (3-TYP), a specific inhibitor of Sirtuin 3 (SIRT3), was used to block the SIRT3/Parkin pathway. Mitophagy in the cartilage sections was evaluated via immunohistochemistry. IL-1β was found to induce chondrocyte apoptosis by inhibiting SIRT3 expression and mitophagy. In addition, inflammatory damage reduced the mitochondrial membrane potential and promoted the production of intracellular reactive oxygen species (ROS), leading to increased mitochondrial division, mitochondrial fusion inhibition, and the consequent mitochondrial damage. In contrast, the MA-5 treatment inhibited excessive ROS production by upregulating mitophagy, maintaining the mitochondrial membrane potential, and reducing mitochondrial apoptosis. After chemically blocking SIRT3 with 3-TYP, Parkin-related mitophagy was also inhibited, an effect that was prevented by pretreatment of the chondrocytes with MA-5, thereby suggesting that SIRT3 is upstream of Parkin. Overall, MA-5 was found to enhance the activity of SIRT3, promote Parkin-dependent mitophagy, eliminate depolarized/damaged mitochondria in chondrocytes, and protect cartilage cells. In conclusion, MA-5 inhibits IL-1β-induced oxidative stress and protects chondrocytes by upregulating the SIRT3/Parkin-related autophagy signaling pathway.
Chemoreflex Control as the Cornerstone in Immersion Water Sports: Possible Role on Breath-Hold
Authors: Arce-Álvarez Alexis , Salazar-Ardiles Camila , Cornejo Carlos , Paez Valeria , Vásquez-Muñoz Manuel , Stillner-Vilches Katherine , Jara Catherine R. , Ramirez-Campillo Rodrigo , Izquierdo Mikel , Andrade David C.
Published: June 2022

Immersion water sports involve long-term apneas; therefore, athletes must physiologically adapt to maintain muscle oxygenation, despite not performing pulmonary ventilation. Breath-holding (i.e., apnea) is common in water sports, and it involves a decrease and increases PaO2 and PaCO2, respectively, as the primary signals that trigger the end of apnea. The principal physiological O2 sensors are the carotid bodies, which are able to detect arterial gases and metabolic alterations before reaching the brain, which aids in adjusting the cardiorespiratory system. Moreover, the principal H+/CO2 sensor is the retrotrapezoid nucleus, which is located at the brainstem level; this mechanism contributes to detecting respiratory and metabolic acidosis. Although these sensors have been characterized in pathophysiological states, current evidence shows a possible role for these mechanisms as physiological sensors during voluntary apnea. Divers and swimmer athletes have been found to displayed longer apnea times than land sports athletes, as well as decreased peripheral O2 and central CO2 chemoreflex control. However, although chemosensitivity at rest could be decreased, we recently found marked sympathoexcitation during maximum voluntary apnea in young swimmers, which could activate the spleen (which is a reservoir organ for oxygenated blood). Therefore, it is possible that the chemoreflex, autonomic function, and storage/delivery oxygen organ(s) are linked to apnea in immersion water sports. In this review, we summarized the available evidence related to chemoreflex control in immersion water sports. Subsequently, we propose a possible physiological mechanistic model that could contribute to providing new avenues for understanding the respiratory physiology of water sports.
Physiological and Transcriptional Responses to Saline Irrigation of Young ‘Tempranillo’ Vines Grafted Onto Different Rootstocks
Authors: Buesa Ignacio , Pérez-Pérez Juan G. , Visconti Fernando , Strah Rebeka , Intrigliolo Diego S. , Bonet Luis , Gruden Kristina , Pompe-Novak Maruša , de Paz Jose M.
Published: June 2022

The use of more salt stress-tolerant vine rootstocks can be a sustainable strategy for adapting traditional grapevine cultivars to future conditions. However, how the new M1 and M4 rootstocks perform against salinity compared to conventional ones, such as the 1103-Paulsen, had not been previously assessed under real field conditions. Therefore, a field trial was carried out in a young ‘Tempranillo’ (Vitis viniferaL.) vineyard grafted onto all three rootstocks under a semi-arid and hot-summer Mediterranean climate. The vines were irrigated with two kinds of water: a non-saline Control with EC of 0.8 dS m–1and a Saline treatment with 3.5 dS m–1. Then, various physiological parameters were assessed in the scion, and, additionally, gene expression was studied by high throughput sequencing in leaf and berry tissues. Plant water relations evidenced the osmotic effect of water quality, but not that of the rootstock. Accordingly, leaf-level gas exchange rates were also reduced in all three rootstocks, with M1 inducing significantly lower net photosynthesis rates than 1103-Paulsen. Nevertheless, the expression of groups of genes involved in photosynthesis and amino acid metabolism pathways were not significantly and differentially expressed. The irrigation with saline water significantly increased leaf chloride contents in the scion onto the M-rootstocks, but not onto the 1103P. The limitation for leaf Cl–and Na+accumulation on the scion was conferred by rootstock. Few processes were differentially regulated in the scion in response to the saline treatment, mainly, in the groups of genes involved in the flavonoids and phenylpropanoids metabolic pathways. However, these transcriptomic effects were not fully reflected in grape phenolic ripeness, with M4 being the only one that did not cause reductions in these compounds in response to salinity, and 1103-Paulsen having the highest overall concentrations. These results suggest that all three rootstocks confer short-term salinity tolerance to the scion. The lower transcriptomic changes and the lower accumulation of potentially phytotoxic ions in the scion grafted onto 1103-Paulsen compared to M-rootstocks point to the former being able to maintain this physiological response in the longer term. Further agronomic trials should be conducted to confirm these effects on vine physiology and transcriptomics in mature vineyards.
Molecular Structure and Phylogenetic Analyses of the Complete Chloroplast Genomes of Three Medicinal Plants Conioselinum vaginatum, Ligusticum sinense, and Ligusticum jeholense
Authors: Wei Xue-Ping , Zhang Xiao-Yi , Dong Yu-Qing , Cheng Ji-Long , Bai Yun-Jun , Liu Jiu-Shi , Qi Yao-Dong , Zhang Ben-Gang , Liu Hai-Tao
Published: June 2022

Most plants of Ligusticum have an important medicinal and economic value with a long history, Ligusticum sinense and L. jeholense (“Gaoben”) has long been used in traditional Chinese medicine for the treatment of carminative, dispelling cold, dehumidification, and analgesia. While in the market Conioselinum vaginatum (Xinjiang Gaoben) is substitution for Gaoben, and occupies a higher market share. These three Gaoben-related medicinal materials are similar in morphology, and are difficult to distinguish from each other by the commonly used DNA barcodes. The chloroplast genome has been widely used for molecular markers, evolutionary biology, and barcoding identification. In this study, the complete chloroplast genome sequences of C. vaginatum, L. sinense, and L. jeholense were reported. The results showed that the complete chloroplast genomes of these three species have typical quadripartite structures, which were comprised of 148,664, 148,539, and 148,497 bp. A total of 114 genes were identified, including 81 protein-coding genes (PCGs), 29 tRNA genes, and four rRNA genes. Our study indicated that highly variable region ycf2-trnL and accD-ycf4 that can be used as specific DNA barcodes to distinguish and identify C. vaginatum, L. sinense, and L. jeholense. In addition, phylogenetic study showed that C. vaginatum nested in Ligusticum and as a sister group of L. sinense and L. jeholense, which suggested these two genera are both in need of revision. This study offer valuable information for future research in the identification of Gaoben-related medicinal materials and will benefit for further phylogenetic study of Apiaceae.
Identification of QTLs Associated With Agronomic Traits in Tobacco via a Biparental Population and an Eight-Way MAGIC Population
Authors: Liu Yutong , Yuan Guangdi , Si Huan , Sun Ying , Jiang Zipeng , Liu Dan , Jiang Caihong , Pan Xuhao , Yang Jun , Luo Zhaopeng , Zhang Jianfeng , Ren Min , Pan Yi , Sun Kefan , Meng He , Wen Liuying , Xiao Zhiliang , Feng Quanfu , Yang Aiguo , Cheng Lirui
Published: June 2022

Agronomic traits such as plant height (PH), leaf number (LN), leaf length (LL), and leaf width (LW), which are closely related to yield and quality, are important in tobacco (Nicotiana tabacum L.). To identify quantitative trait loci (QTLs) associated with agronomic traits in tobacco, 209 recombinant inbred lines (RILs) and 537 multiparent advanced generation intercross (MAGIC) lines were developed. The biparental RIL and MAGIC lines were genotyped using a 430 K single-nucleotide polymorphism (SNP) chip assay, and their agronomic traits were repeatedly evaluated under different conditions. A total of 43 QTLs associated with agronomic traits were identified through a combination of linkage mapping (LM) and association mapping (AM) methods. Among these 43 QTLs, three major QTLs, namely qPH13-3, qPH17-1, and qLW20-1, were repeatedly identified by the use of various genetically diverse populations across different environments. The candidate genes for these major QTLs were subsequently predicted. Validation and utilization of the major QTL qLW20-1 for the improvement of LW in tobacco were investigated. These results could be applied to molecular marker-assisted selection (MAS) for breeding important agronomic traits in tobacco.
Perennial Cotton Ratoon Cultivation: A Sustainable Method for Cotton Production and Breeding
Authors: Zhang Xin , Yang Qian , Zhou Ruiyang , Zheng Jie , Feng Yan , Zhang Baohong , Jia Yinhua , Du Xiongming , Khan Aziz , Zhang Zhiyong
Published: June 2022

Cotton production is challenged by high costs with multiple management and material inputs including seed, pesticide, and fertilizer application. The production costs can be decreased and profits can be increased by developing efficient crop management strategies, including perennial cotton ratoon cultivation. This review focuses on the role of ratoon cultivation in cotton productivity and breeding. In areas that are frost-free throughout the year, when the soil temperature is suitable for cotton growth in spring, the buds of survived plants begin to sprout, and so their flowering and fruiting periods are approximately 4–6 weeks earlier than those of sown cotton. Due to the absence of frost damage, the ratoon cotton continues to grow, and the renewed plants can offer a higher yield than cotton sown in the following season. Moreover, ratoon cultivation from the last crop without sowing can help conserve seeds, reduce labor inputs, and reduce soil and water loss. In this review, the preservation of perennial cotton germplasm resources, the classification and genome assignment of perennial species in the cotton gene pools, and effective strategies for the collection, preservation, identification, and utilization of perennial cotton germplasms are discussed. Ratoon cultivation is the main driver of cotton production and breeding, especially to maintain male sterility for the utilization and fixation of heterosis. Ratoon cultivation of cotton is worth adopting because it has succeeded in Brazil, China, and India. Therefore, taking advantages of the warm environment to exploit the indeterminant growth habit of perennial cotton for breeding would be an efficiency-increasing, cost-saving, and eco-friendly approach in frost-free regions. In the future, more attention should be given to ratooning perennial cotton for breeding male-sterile lines.
Establishment and Optimization of Molecular Cytogenetic Techniques (45S rDNA-FISH, GISH, and Fiber-FISH) in Kiwifruit (Actinidia Lindl.)
Authors: Zhao Yang , Deng Honghong , Chen Yao , Li Jihan , Chen Silei , Li Chunyan , Mu Xue , Hu Zhongrong , Li Kunming , Wang Weixing
Published: June 2022

The kiwifruit (Actinidia chinensis) has long been regarded as “the king of fruits” for its nutritional importance. However, the molecular cytogenetics of kiwifruit has long been hampered because of the large number of basic chromosome (x = 29), the inherent small size and highly similar morphology of metaphase chromosomes. Fluorescence in situ hybridization (FISH) is an indispensable molecular cytogenetic technique widely used in many plant species. Herein, the effects of post-hybridization washing temperature on FISH, blocking DNA concentration on genomic in situ hybridization (GISH), extraction method on nuclei isolation and the incubation time on the DNA fiber quality in kiwifruit were evaluated. The post-hybridization washing in 2 × saline sodium citrate (SSC) solution for 3 × 5 min at 37°C ensured high stringency and distinct specific FISH signals in kiwifruit somatic chromosomes. The use of 50 × blocking DNA provided an efficient and reliable means of discriminating between chromosomes derived from in the hybrids of A. chinensis var. chinensis (2n = 2x = 58) × A. eriantha (2n = 2x = 58), and inferring the participation of parental genitors. The chopping method established in the present study were found to be very suitable for preparation of leaf nuclei in kiwifruit. A high-quality linear DNA fiber was achieved by an incubation of 20 min. The physical size of 45S rDNA signals was approximately 0.35–0.40 μm revealed by the highly reproducible fiber-FISH procedures established and optimized in this study. The molecular cytogenetic techniques (45S rDNA-FISH, GISH, and high-resolution fiber-FISH) for kiwifruit was for the first time established and optimized in the present study, which is the foundation for the future genomic and evolutionary studies and provides chromosomal characterization for kiwifruit breeding programs.
From COVID-19 Pandemic to Entrepreneurial Behavior: The Mediating Effect of Proactive Personality and the Moderating Role of Anticipated Regret
Authors: Jiatong Wang , Murad Majid , Bajun Fu , Syed Nausheen , Munir Muhammad
Published: June 2022

COVID-19 is a global public health issue that poses a challenge to the education sector. The pandemic has a devastating impact on student entrepreneurial behavior and their mental health. This study aimed to examine the impact of COVID-19 on the student entrepreneurial intention/behavioral model with a mediating effect of proactive personality and the moderating role of anticipated regret. The sample of the study comprised 345 university students from Pakistan. Data were collected using a self-report and other report survey questionnaires. The hypotheses were investigated using the partial least squares structural equation modeling (PLS–SEM) approach. According to the findings, COVID-19 perception has a negative and significant impact on the student entrepreneurial intention/behavior model. Meanwhile, findings show that a proactive personality significantly mediates the relationship between COVID-19 perception and entrepreneurial intention. The results show that anticipated regret moderates the relationship between entrepreneurial intention and entrepreneurial behavior in a favorable and significant way. Furthermore, discussion and implications were also discussed in this article.
Elite Players Invest Additional Time for Making Better Embodied Choices
Authors: Hinz Matthias , Lehmann Nico , Musculus Lisa
Published: June 2022

Expert athletes are determined to make faster and better decisions, as revealed in several simple heuristic studies using verbal reports or micro-movement responses. However, heuristic decision-making experiments that require motor responses, also being considered as the embodied-choice experiments, are still underrepresented. Furthermore, it is less understood how decision time and confidence depend on the type of embodied choices players make. To scrutinize the decision-making processes (i.e., decision time, decision confidence), this study investigated the embodied choices of male athletes with different expertise in a close-to-real-life environment; 22 elite (Mage = 17.59 yrs., SD = 3.67), and 22 amateur (Mage = 20.71 yrs., SD = 8.54) team handball players performed a sport-specific embodied-choice test. Attack sequences (n = 32) were shown to the players, who had to choose between four provided options by giving a respective sport-specific motor response. We analyzed the frequencies of specific choices and the best choice, as well as the respective decision time and decision confidence. Elite and amateur players differed in the frequencies of specific choices (i.e., forward/tackling; passive blocking), and elite players made the best choice more often. Slower decision times of elite players were revealed in specific choices and in best choices, the confidence of decisions was rated equally high by both player groups. Indications are provided that elite players make better choices rather slower, instead of faster. We suppose this is due to specific sensorimotor interactions and speed-accuracy-tradeoffs in favor of accuracy in elite players. Our findings extend expert decision-making research by using an embodied-choice paradigm, highlighting considerations of decision time and confidence in future experiments.
Applying the Dynamic Dual Pathway Model of Approach Coping to Collective Action Among Advantaged Group Allies and Disadvantaged Group Members
Authors: Radke Helena R. M. , Kutlaca Maja , Becker Julia C.
Published: June 2022

We apply the dynamic dual pathway model of approach coping to understanding the predictors of future collective action among a sample of advantaged group allies and disadvantaged group members who were attending a protest. We propose that problem-focused approach coping (i.e., group efficacy beliefs) would be a stronger predictor of future collective action among disadvantaged compared to advantaged group members, and emotion-focused approach coping (i.e., group-based anger) would be a stronger predictor of future collective action among advantaged compared to disadvantaged group members. Data was collected from LGBTIQ+ and heterosexual people (N= 189) protesting as part of the 2019 Christopher Street Day Parade in Cologne, Germany. We found that increased group efficacy predicted intentions to engage in future collective action for the rights of sexual minorities among LGBTIQ+ but not heterosexual participants. Increased group-based anger was a predictor of future collective action intentions regardless of which group the participants belonged to. Our findings extend the dynamic dual pathway model by applying it to a sample of advantaged group allies and disadvantaged group members attending a protest using a multiple perspectives approach.
Emotional Self-Care: Exploring the Influencing Factors Among Individuals With Cancer
Authors: Sebastian Ann Tresa , Rajkumar Eslavath , John Romate , Daniel Monica , George Allen Joshua , Greeshma Rajgopal , James Treasa
Published: June 2022

Cancer is a leading source of distress and fatality worldwide. Cancer-related aberrant cell proliferation causes excruciating pain and impairment. To cope with pain and manage symptoms and illness, pharmaceutical and non-pharmacological options are available. Self-care behaviors are recognized as a key source in symptom management and improving quality adherence to treatment among the current non-pharmacological strategies. The intervention measures to improve self-care were hardly impacted because of the narrow focus on physical self-care. Bringing in emotional self-care and addressing the individual's emotional health can enhance the effectiveness of interventions on a holistic level. Hence, this study has attempted to explore the factors that influence emotional self-care among individuals with cancer. Following an exploratory research design, the data were collected from 15 participants (4 men and 11 women) using purposive sampling and semi-structured interviews. Through thematic analysis, eight major themes were identified: physiological factors, social factors, family factors, psychological factors, individual factors, socioeconomic factors, cultural factors, and spiritual factors. The findings explained the emotional self-care among patients with cancer and how different identified factors influence their emotional self-care practices.
Alternatives to Gold Standard Diagnostic Tools for Distinguishing “Natural Kinds” on the Autism Spectrum
Authors: Philippe Anne
Published: June 2022

Next-generation sequencing techniques have accelerated the discovery of rare mutations responsible for autism spectrum disorder (ASD) in genes involved in a large number of physiological processes, including the control of gene expression, chromatin remodeling, signaling pathways, synaptic scaffolding, neurotransmitter receptors, and lipid metabolism. Genetic diagnosis provides subjects with an explanation of the cause of their disorder. However, it does not, or at least does not yet, shed light on the psychopathological phenomena specific to the individual. It could be hypothesized that each physiological impact of a mutation corresponds to a specific psychopathological phenomenon of ASD, i.e., “a psychopathological natural kind”. We discuss here the difficulties identifying this specificity of underlying psychopathology in individuals with ASD due to a rare mutation with a major effect. A comparison of Newson's pathological demand avoidance and Wing's Asperger's syndrome with Asperger's autistic psychopathy highlights different ways of approaching psychopathological descriptions and diagnosis, by focusing on either common or unusual features. Such a comparison calls into question the principles of clinical research recommended by Falret for characterizing “disease individuality” of ASD due to a rare mutation.
Impact and Risk of Moral Injury Among Deployed Veterans: Implications for Veterans and Mental Health
Authors: Boscarino Joseph A. , Adams Richard E. , Wingate Tiah J. , Boscarino Joseph J. , Urosevich Thomas G. , Hoffman Stuart N. , Kirchner H. Lester , Figley Charles R. , Nash William P.
Published: June 2022

The impact of “moral injury” (MI) among deployed veterans, defined as actions in combat that violate a veteran's moral beliefs and result in psychological distress, has increasingly become a significant clinical concern separate from other trauma- and stressor-related disorders. MI involves severe distress over violations of core beliefs often followed by feelings of guilt and conflict and is common among veterans with PTSD. While the psychological impact of PTSD is well-documented among veterans, this has been done less so with respect to MI. We studied MI among 1,032 deployed veterans who were outpatients in a large non-profit multi-hospital system in central Pennsylvania. The study included active duty and Guard/Reserve members, as well as veterans who were not Department of Veterans Affairs (VA) service users. Our hypothesis was that, controlling for other risk factors, veterans with high MI would have current mental disorders. Our secondary hypothesis was that MI would be associated with other psychopathologies, including chronic pain, sleep disorders, fear of death, anomie, and use of alcohol/drugs to cope post deployment. Most veterans studied were deployed to Vietnam (64.1%), while others were deployed to post-Vietnam conflicts in Iraq and Afghanistan and elsewhere. Altogether, 95.1% of the veterans were male and their mean age was 61.6 years (SD = 11.8). Among the veterans, 24.4% had high combat exposure, 10.9% had PTSD, 19.8% had major depressive disorder, and 11.7% had a history of suicidal thoughts. Based on the Moral Injury Events Scale (MIES), 25.8% had high MI post deployment, defined as a score above the 75th percentile. Results show that high MI among veterans was associated with current global mental health severity and recent mental health service use, but not suicidal thoughts. In addition, as hypothesized, MI was also associated with pain, sleep disorders, fear of death, anomie, use of alcohol/drugs to cope post-deployment, and poor unit support/morale during deployment. Deployed veterans with MI are more likely to have current mental health disorders and other psychological problems years after deployment. Further research is advised related to the screening, assessment, treatment, and prevention of MI among veterans and others after trauma exposures.
Variability of the Center of Mass in Trained Triathletes in Running After Cycling: A Preliminary Study Conducted in a Real-Life Setting
Authors: Evans Stuart A. , James Daniel , Rowlands David , Lee James B.
Published: June 2022

While the sport of short-distance (Sprint) triathlon provides an opportunity to research the effect of the center of mass (CoM) when cycling and running, much remains to be done. The literature has failed to consistently or adequately report how changes to hand position influence subsequent running as inferred by the magnitude of CoM acceleration. The demands of cycle training in a drops and aerodynamic position followed by running remain unquantified in Sprint Distance triathlon. Thus, far data collected indicate that the cycle to run transition (T2) is important for overall race success. While many age-groupers participate in Sprint Distance triathlon, the lack of T2 based research make comparisons between cycle hand position and ensuing running difficult. The motion of the human body when cycling and running in triathlon can be described by the motion of its CoM in a local coordinate system. Unobtrusive wearable sensors have proven to be an informative resource to monitor the magnitude of CoM accelerations in running. However, the extent to which they are used in cycling is unclear. Therefore, the aim of the present study was to analyse the temporal magnitudes of CoM acceleration when cycling position and cadence is changed and to analyse these effects on running after cycling. Ten recreational triathletes completed two 20 km cycling trials at varied cadence in a drops position (parts of the handlebars that curve outward, CycleDrops) and an aerodynamic position (arms bent, forearms parallel to the ground, CycleAero) immediately followed by a 5 km run at self-selected pace. Torso kinematics by way of CoM acceleration magnitude were captured in a typical training setting using a triaxial accelerometer. CoM acceleration was quantified in m/s2 and variability was measured by the coefficient of variation (CV) and root mean square (RMS). Results from CycleAero indicated that acceleration of the CoM in longitudinal (CV = 1%) and mediolateral directions (CV = 3%) was significantly reduced (p < 0.001) compared to CycleDrops. As for rate of perceived exertion (RPE), a significant difference was observed with triathletes reporting higher values in CycleAero alongside a greater CoM acceleration magnitude in the anteroposterior direction. The CoM varied significantly from RunAero with less longitudinal (CV = 0.2, p < 0.001) and mediolateral acceleration observed (CV = 7.5%, p < 0.001) compared to RunDrops. Although greater longitudinal acceleration was observed in the initial 1 km epoch of RunAero, triathletes then seemingly adjusted their CoM trajectory to record lower magnitudes until completion of the 5 km run, completing the run quicker compared to RunDrops (22.56 min1 ± 0.2, 23.34 min1 ± 0.5, p < 0.001, CV = 1.3%). Coaches may look to use triaxial accelerometers to monitor performance in both cycling and running after cycling.
Intralesional Marginal Resection for Osteoblastoma in the Mobile Spine: Experience From a Single Center
Authors: Cao Shiliang , Chen Keyuan , Jiang Liang , Wei Feng , Liu Xiaoguang , Liu Zhongjun
Published: June 2022

Osteoblastoma (OB) is a benign bone tumor with aggressive behavior and a tendency for local recurrence. The appropriate surgical strategy for spinal OB remains unclear. This retrospective study aimed to verify the clinical efficacy and safety of intralesional marginal resection of OB in the mobile spine. We enrolled 50 consecutive patients with spinal OB between January 2009 and December 2019. The tumors were staged based on the Enneking system, with 21 and 29 lesions being determined as stage 2 (St.2) and stage 3 (St.3), respectively. Among them, 42 patients underwent intralesional marginal resection, five underwent extensive curettage, and three underwent en bloc resection successfully since their lesions were limited to the posterior element in a single vertebra. We analyzed clinical characteristics, perioperative and follow-up images, surgical details, and follow-up data. Within a median follow-up duration of 50 (range: 24–160) months, six (12.0%) patients had local recurrence. The recurrence rates among patients who underwent intralesional marginal resection, curettage, en bloc resection were 7.1%(3/42), 60.0%(3/5), and 0%(0/3), respectively. The recurrence rate of intralesional marginal resection of St.3 lesions was slightly higher than that of St.2 lesions (7.7%[2/26] vs. 6.3%[1/16]). There were 16(38.1%), 3(60.0%), and 0 patients with surgical complications among those who underwent intralesional marginal resection, curettage, and en bloc resection, respectively. Local recurrence was observed in five (5/14, 35.7%) patients who had vertebral artery extension and in none who did not have vertebral artery extension (p = 0.02). Our findings suggest that intralesional marginal resection could be an appropriate treatment choice for patients with spinal OB, both St.2 and St.3 lesions, with an acceptable local recurrence rate and a low risk of complications. Vertebral artery extension could be a strong risk factor for local recurrence in patients with spinal OB.
Characterizing the Effect of the Lysine Deacetylation Modification on Enzyme Activity of Pyruvate Kinase I and Pathogenicity of Vibrio alginolyticus
Authors: Xu Zhou , Wang Linjing , Wang Xudong , Wan Mingyue , Tang Mei , Ding Yu
Published: June 2022

Pyruvate kinase I (PykF) is one of the key enzymes of glycolysis and plays a crucial role in bacterial metabolism. Several acetylation sites of Vibrio alginolyticus PykF were reported in previous studies and then 11 sites were first verified in this study, however, the specific roles of PykF acetylation remains unclear. Overlap-PCR and homologous recombination were implied to delete V. alginolyticus pykF gene and constructed complementary strains of site-directed mutagenesis for the further research focus on the deacetylation regulation on PykF. The results showed that the pyruvate kinase activity was sharply suppressed in the deacetylation status of K52, K68, and K317 of PykF, as well as the extracellular protease activity was significantly decreased in the deacetylation status of K52 and K68, but not induced with K317. Moreover, the growth rates of V. alginolyticus were not influenced with these three deacetylation sites. The ΔpykF mutant exhibited a 6-fold reduction in virulence to zebrafish. Site-directed mutations of K52R and K68R also showed reduced virulence while mutations of K317R didn't. The in vitro experiments showed that PykF was acetylated by acetyl phosphate (AcP), with the increase of incubation time by AcP, the acetylation level of PykF increased while the enzyme activity of PykF decreased correspondingly. Besides, PykF was deacetylated by CobB deacetylase and in result that the deacetylation was significantly down-regulated while the pyruvate kinase activity of PykF increased. Moreover, deletion of cobB gene had no significant difference in pyruvate kinase activity. These results confirm that CobB can regulate the acetylation level and pyruvate kinase activity of PykF. In summary, the results of this study provide a theoretical basis for further understanding of the deacetylation modification of PykF. It provides a new idea for the prevention and cure of vibriosis.