bluuebunny/crossref_metadata_2025 huggingface dataset:
Changes in attitudes and behaviors supportive of maternal and newborn health in Ethiopia: an evaluative case study
Authors: Story William T. , Amare Yared , Vaz Lara M.E. , Gardner Heather , Tura Halkeno , Snetro Gail , Kinney Mary V. , Wall Steve , Bekele Abeba
Published: May 2021

Background

Ethiopia’s high neonatal mortality rate led to the government’s 2013 introduction of Community-Based Newborn Care (CBNC) to bring critical prevention and treatment interventions closer to communities in need. However, complex behaviors that are deeply embedded in social and cultural norms continue to prevent women and newborns from getting the care they need. A demand creation strategy was designed to create an enabling environment to support appropriate maternal, newborn, and child health (MNCH) behaviors and CBNC. We explored the extent to which attitudes and behaviors during the prenatal and perinatal periods varied by the implementation strength of the Demand Creation Strategy for MNCH-CBNC.

Methods

Using an embedded, multiple case study design, we purposively selected four kebeles (villages) from two districts with different levels of implementation strength of demand creation activities. We collected information from a total of 150 key stakeholders across kebeles using multiple qualitative methods including in-depth interviews, focus group discussions, and illness narratives; sessions were transcribed into English and coded using NVivo 10.0. We developed case reports for each kebele and a final cross-case report to compare results from high and low implementation strength kebeles.

Results

We found that five MNCH attitudes and behaviors varied by implementation strength. In high implementation strength kebeles women felt more comfortable disclosing their pregnancy early, women sought antenatal care (ANC) in the first trimester, families did not have fatalistic ideas about newborn survival, mothers sought care for sick newborns in a timely manner, and newborns received care at the health facility in less than an hour. We also found changes across all kebeles that did not vary by implementation strength, including male engagement during pregnancy and a preference for giving birth at a health facility.

Conclusions

Findings suggest that a demand creation approach—combining participatory approaches with community empowering strategies—can promote shifts in behaviors and attitudes to support the health of mothers and newborns, including use of MNCH services. Future studies need to consider the most efficient level of intervention intensity to make the greatest impact on MNCH attitudes and behaviors.
Quantitative visual tests in primary open-angle glaucoma patients according to three different lights with different color-rendering index
Authors: Kim Sang Woo , Go YoungWook , Kang Sang-Ook , Lee Chang Kyu
Published: May 2021

Purpose

To compare quantitative visual tests, such as visual acuity, contrast sensitivity, and color vision tests in patients with primary open-angle glaucoma (POAG) patients according to three different light systems with different color-rendering index (CRI).

Methods

This was a cross-sectional study of 36 eyes in 36 patients with POAG. Three different light systems consisting of a 3-band fluorescent lamp (CRI 80), a white LED (CRI 75), and a quantum dot LED (CRI > 95) were used. All lights had the same illuminance of 230 lx to exclude illuminance effects. The visual testing included best-corrected visual acuity (BCVA) using an ETDRS chart, a CSV-1000E contrast test, and a color test performed by the Farnsworth Munsell 100-hue test.

Results

There was no significant difference in BCVA (p = 0.86). There were no significant differences in the detail contrast tests according to the three light systems (p = 0.95, p = 0.94, p = 0.94, respectively, p = 0.64). There was significant difference between the three light systems in color test (p = 0.042). The color test scores with a quantum dot LED were significantly lower than those of the white LED and 3-band fluorescent lamp (p = 0.03 and 0.047, respectively).

Conclusions

POAG patients did not show significant differences in visual acuity scores and contrast test scores, expressed as black and white symbols, according to the different light systems. However, POAG patients tested under a quantum dot LED (CRI > 95) could distinguish color differences better than in the other light systems.
Sex differences in risk factors for depressive symptoms in patients with COPD: The 2014 and 2016 Korea National Health and Nutrition Examination Survey
Authors: Choi Ji Soo , Kwak Se Hyun , Son Nak-Hoon , Oh Jae Won , Lee San , Lee Eun Hye
Published: May 2021

Background

Although depression is a common comorbidity of chronic obstructive pulmonary disease (COPD), the role of sex remains unexplored. We evaluated sex differences of risk factors of depressive symptoms in adults with COPD.

Methods

This was a population-based cross-sectional study using data from the 2014 and 2016 Korea National Health and Nutrition Examination Survey. Spirometry was used to identify patients with COPD, defined as a FEV1/FVC ratio < 0.7. Presence of depressive symptoms was defined as a total score ≥ 5 on the Patient Health Questionnaire-9.

Results

17.8% of participants expressed depressive symptoms. Relative regression analysis revealed that female sex (RR 2.38; 95% CI 1.55–3.66; p < 0.001), living alone (RR 1.46; 95% CI 1.08–1.97; p = 0.013), current smoker (RR 1.70; 95% CI 1.15–2.52; p = 0.008), underweight (RR 1.58 95% CI 1.00–2.49; p = 0.049), and GOLD Stage III/IV (RR 1.92; 95% CI 1.19–3.09; p = 0.007) were the risk factors for depressive symptoms. Low income, living alone, multiple chronic disorders, and low BMI were risk factors of depressive symptoms in male, whereas low educational attainment, urban living, and current smoking were risk factors in female.

Conclusions

Female sex is a main risk factor of depressive symptoms in adults with COPD. As risk factors of depressive symptoms in COPD patients vary according to their sex, different approaches are needed to manage depression in males and females with COPD.
Immunoglobulin G4-related periodontitis: case report and review of the literature
Authors: Zhang Jinmei , Zhao Lei , Zhou Jieyu , Dong Wei , Wu Yafei
Published: May 2021

Background

Immunoglobulin G4-related disease (IgG4-RD) is a chronic inflammatory systemic disease of unknown etiology that can affect one or multiple organs. The disease can mimic many infectious and inflammatory diseases, mainly causing organ enlargement or hyperplasia. Its diagnosis primarily relies on clinical, serologic, and histological features (lymphoplasmacytic infiltrates, storiform fibrosis, and obliterative phlebitis of IgG4 + plasma cells). Here, we report a rare case of IgG4-related periodontitis, and review the relevant literatures.

Case presentation

A 38-year-old Chinese man visited the Department of Periodontics with gingival enlargement, loose teeth, and tooth loss. The patient had very poor oral hygiene and a large amount of calculus. Gingivae were edematous with deep periodontal pockets and attachment loss. Panoramic radiography showed alveolar bone loss. Serologic examination showed that IgG was 23.70 g/L and IgG4 concentration was 2.800 g/L. There was significant lymphoplasmacytic infiltration, a storiform pattern of fibrosis, and mitotic figures with hematoxylin and eosin staining; immunohistochemical staining showed 10 scattered IgG4-positive plasma cells in a high-power field. The patient was diagnosed as IgG4-related periodontitis. He received a course of corticosteroids with periodontal therapy, and the enlargement was significantly improved without recurrence.

Conclusion

IgG4-RD in the oral and maxillofacial region mainly involves salivary glands, but this rare case was characterized by enlarged gingivae. The differential diagnosis of IgG4-RD should be based on the clinical features and serologic (IgG4) and histopathological examinations. Corticosteroid therapy is effective for most IgG4-RD patients. Taken together, we hope this case report and the literature review can help dentists to improve their understanding of the IgG4-RD.
Oral contraceptives and hypertension in women: results of the enrolment phase of Tabari Cohort Study
Authors: Afshari Mahdi , Alizadeh-Navaei Reza , Moosazadeh Mahmood
Published: May 2021

Background

The association between oral contraceptives (OCP) and hypertension has been reported in the literature with controversial results. According to the growing use of OCPs among women in Iran, this study aims to investigate the association between the duration of the OCP consumption and risk of hypertension among Iranian women.

Methods

In the current study, the data collected during the enrolment phase of the Tabari cohort were analyzed. Of 6106 women recruited in the cohort, 133 pregnant women were excluded. Epidemiological variables were collected using pre-designed questionnaires as well as the health insurance evidences. In addition, blood pressure and anthropometric factors were measured based on the standard guidelines. Chi square and partial correlation tests as well as logistic regression models were applied for data analysis.

Results

Frequency of oral contraceptive use among 35–70 year-old women in Tabari cohort study (TCS) was 42.2% (2520/5973). Hypertension was observed among 25% (1793/5973) of them. The adjusted odds ratio for OCP use was 1.23 (95% confidence interval: 1.08, 1.40, p = 0.002). The corresponding odds ratios for 61–120 months and more than 120 months OCP use were 1.39 (1.12,1.73) and 1.47 (1.16,1.87) respectively.

Conclusions

Oral contraceptives especially in long term use can be associated with hypertension.
Stakeholders' perspectives on research integrity training practices: a qualitative study
Authors: Pizzolato Daniel , Dierickx Kris
Published: May 2021

Background

Even though research integrity (RI) training programs have been developed in the last decades, it is argued that current training practices are not always able to increase RI-related awareness within the scientific community. Defining and understanding the capacities and lacunas of existing RI training are becoming extremely important for developing up-to-date educational practices to tackle present-day challenges. Recommendations on how to implement RI education have been primarily made by selected people with specific RI-related expertise. Those recommendations were developed mainly without consulting a broader audience with no specific RI expertise. Moreover, the academic literature lacks qualitative studies on RI training practices. For these reasons, performing in-depth focus groups with non-RI expert stakeholders are of a primary necessity to understand and outline how RI education should be implemented.

Methods

In this qualitative analysis, different focus groups were conducted to examine stakeholders’ perspectives on RI training practices. Five stakeholders' groups, namely publishers and peer reviewers, researchers on RI, RI trainers, PhDs and postdoctoral researchers, and research administrators working within academia, have been identified to have a broader overview of state of the art.

Results

A total of 39 participants participated in five focus group sessions. Eight training-related themes were highlighted during the focus group discussions. The training goals, timing and frequency, customisation, format and teaching approach, mentoring, compulsoriness, certification and evaluation, and RI-related responsibilities were discussed. Although confirming what was already proposed by research integrity experts in terms of timing, frequency, duration, and target audience in organising RI education, participants proposed other possible implementations strategies concerning the teaching approach, researchers' obligations, and development an evaluation-certification system.

Conclusions

This research aims to be a starting point for a better understanding of necessary, definitive, and consistent ways of structuring RI education. The research gives an overview of what has to be considered needed in planning RI training sessions regarding objectives, organisation, and teaching approach.
Potentially inappropriate medications and medication combinations before, during and after hospitalizations: an analysis of pathways and determinants in the Swiss healthcare setting
Authors: Migliazza Kevin , Bähler Caroline , Liedtke Daniel , Signorell Andri , Boes Stefan , Blozik Eva
Published: May 2021

Background

A hospitalization phase represents a challenge to medication safety especially for multimorbid patients as acute medical needs might interact with pre-existing medications or evoke adverse drug effects. This project aimed to examine the prevalence and risk factors of potentially inappropriate medications (PIMs) and medication combinations (PIMCs) in the context of hospitalizations.

Methods

Analyses are based on claims data of patients (≥65 years) with basic mandatory health insurance at the Helsana Group, and on data from the Hirslanden Swiss Hospital Group. We assessed PIMs and PIMCs of patients who were hospitalized in 2013 at three different time points (quarter prior, during, after hospitalization). PIMs were identified using the PRISCUS list, whereas PIMCs were derived from compendium.ch. Zero-inflated Poisson regression models were applied to determine risk factors of PIMs and PIMCs.

Results

Throughout the observation period, more than 80% of patients had at least one PIM, ranging from 49.7% in the pre-hospitalization, 53.6% in the hospitalization to 48.2% in the post-hospitalization period. PIMCs were found in 46.6% of patients prior to hospitalization, in 21.3% during hospitalization, and in 25.0% of patients after discharge. Additional medication prescriptions compared to the preceding period and increasing age were the main risk factors, whereas managed care was associated with a decrease in PIMs and PIMCs.

Conclusion

We conclude that a patient’s hospitalization offers the possibility to increase medication safety. Nevertheless, the prevalence of PIMs and PIMCs is relatively high in the study population. Therefore, our results indicate a need for interventions to increase medication safety in the Swiss healthcare setting.
Effects of isoflurane and sevoflurane alone and in combination with butorphanol or medetomidine on the bispectral index in chickens
Authors: Velasco Gallego Maria Luisa , Martin Jurado Olga , Hatt Jean-Michel
Published: May 2021

Background

The bispectral index (BIS) is an anaesthesia monitoring technique able to assess the level of central nervous system depression in humans and various animal species. In birds, it has been validated in chickens undergoing isoflurane anaesthesia. The aim of this study was to evaluate in an avian species the influence of isoflurane and sevoflurane on BIS, each at different minimum anaesthetic concentrations (MAC) multiples, alone or combined with butorphanol or medetomidine. Ten chickens (5 males and 5 females) underwent general anaesthesia with isoflurane or sevoflurane alone, and combined with either intramuscular administration of butorphanol (1 mg/kg) or medetomidine (0.1 mg/kg), in a prospective and cross-over study (i.e., 6 treatments per animal). BIS measurements were compared to heart rate (HR), non-invasive blood pressure (NIBP) and to a visual analogue scale (VAS) of anaesthesia depth.

Results

HR was significantly increased, and both NIBP and VAS were significantly reduced, with higher gas concentrations. NIBP (but not HR or VAS) was additionally affected by the type of gas, being lower at higher concentrations of sevoflurane. Butorphanol had no additional effect, but medetomidine led to differences in HR, NIBP, and in particular a reduction in VAS. With respect to deeper level of hypnosis at higher concentrations and the absence of difference between gases, BIS measurements correlated with all other measures (except with HR, where no significant relationship was found) The difference in BIS before (BISpre) and after stimulation (BISpost) did not remain constant, but increased with increasing MAC multiples, indicating that the BISpost is not suppressed proportionately to the suppression of the BISpre values due to gas concentration. Furthermore, neither butorphanol nor medetomidine affected the BIS.

Conclusions

The difference of degree of central nervous system depression monitored by BIS compared with neuromuscular reflexes monitored by VAS, indicate that BIS records a level of anaesthetic depth different from the one deducted from VAS monitoring alone. BIS provided complementary information such as that medetomidine suppressed spinal reflexes without deepening the hypnotic state. As a consequence, it is concluded that BIS improves the assessment of the level of hypnosis in chickens, improving anaesthesia monitoring and anaesthesia quality in this species.
Influences on tidal channel and aquaculture shrimp pond water chemical composition in Southwest Bangladesh
Authors: Dietrich Matthew , Ayers John C.
Published: May 2021

Detailed geochemical studies of both major and minor elements in Bangladesh surface waters are sparse, particularly in shrimp aquaculture pond environments. Therefore, water samples from shrimp aquaculture ponds and tidal channels were collected in high precipitation (July) and low precipitation (May) months from 2018–2019 in Southwest Bangladesh and analyzed for complete water chemistry. Selenium (Se) and arsenic (As) were elevated above WHO guidelines in 50% and ~ 87% of samples, respectively, but do not show any recognizable spatial patterns. Shrimp pond and tidal channel water compositions in the dry season (May) are similar, illustrating their connectivity and minimal endogenous effects within shrimp ponds. Tidal channels are less saline in July than shrimp ponds still irrigated by tidal channels, suggesting that either farmers limit irrigation to continue farming saltwater shrimp, or the irrigation flux is low and leads to a lag in aquaculture-tidal channel compositional homogenization. δ18O and δ2H isotopic compositions from samples in May of 2019 reveal tidal channel samples are closer to the local meteoric water line (LMWL) than shrimp pond samples, because of less evaporation. However, evaporation in May shrimp ponds has a minimal effect on water composition, likely because of regular drainage/exchange of pond waters. Dissolved organic carbon (DOC) is positively correlated with both δ18O and δ2H in shrimp ponds, suggesting that as evaporation increases, DOC becomes enriched. Multiple linear regression reveals that As and Se can be moderately predicted (adjusted R2 values between 0.4 and 0.7, p < 0.01) in surface waters of our study with only 3–4 independent predictor variables (e.g., Ni, V and DOC for Se prediction; Cu, V, Ni and P for As prediction). Thus, this general approach should be followed in other regions throughout the world when measurements for certain hazardous trace elements such as Se and As may be lacking in several samples from a dataset.
Gold standard for nutrition: a review of human milk oligosaccharide and its effects on infant gut microbiota
Authors: Zhang Shunhao , Li Tianle , Xie Jing , Zhang Demao , Pi Caixia , Zhou Lingyun , Yang Wenbin
Published: May 2021

Human milk is the gold standard for nutrition of infant growth, whose nutritional value is mainly attributed to human milk oligosaccharides (HMOs). HMOs, the third most abundant component of human milk after lactose and lipids, are complex sugars with unique structural diversity which are indigestible by the infant. Acting as prebiotics, multiple beneficial functions of HMO are believed to be exerted through interactions with the gut microbiota either directly or indirectly, such as supporting beneficial bacteria growth, anti-pathogenic effects, and modulation of intestinal epithelial cell response. Recent studies have highlighted that HMOs can boost infants health and reduce disease risk, revealing potential of HMOs in food additive and therapeutics. The present paper discusses recent research in respect to the impact of HMO on the infant gut microbiome, with emphasis on the molecular basis of mechanism underlying beneficial effects of HMOs.
Ventricular tachycardia with epicardial and pericardial fibrosis 6 months after resolution of subclinical COVID-19: a case report
Authors: Solaimanzadeh Jonathan , Freilich Aaron , Sood Michael R
Published: May 2021

Background

Coronavirus disease 2019 (COVID-19) has been shown to have extensive effects on the cardiovascular system. Its long-term cardiac manifestations, however, remain unclear.

Case presentation

We report the case of a Caucasian patient with a mild and self-limited presentation of COVID-19, with subsequent development, months later, of exertional dyspnea and non-sustained ventricular tachycardia, long after resolution of his illness and after returning to aerobic exercise. The patient had normal screening tests including electrocardiogram (ECG) and echocardiogram 4 months after his illness. Cardiac magnetic resonance imaging demonstrated epicardial and pericardial fibrosis of the right ventricle free wall and outflow tract and the pericardium over the anterior wall, 6 months following the initial infection. First abnormal ECG was recorded at month 7 following illness.

Conclusions

This case suggests an insidious and possible long-term cardiac involvement and reflects the challenges in traditional workups and screening modalities in identifying cardiac involvement in COVID-19.
Effects of cofD gene knock-out on the methanogenesis of Methanobrevibacter ruminantium
Authors: Ma Jian , Wang Xueying , Zhou Ting , Hu Rui , Zou Huawei , Wang Zhisheng , Tan Cui , Zhang Xiangfei , Peng Quanhui , Xue Bai , Wang Lizhi
Published: May 2021

This study aimed to investigate the effects of cofD gene knock-out on the synthesis of coenzyme F420 and production of methane in Methanobrevibacter ruminantium (M. ruminantium). The experiment successfully constructed a cofD gene knock-out M. ruminantium via homologous recombination technology. The results showed that the logarithmic phase of mutant M. ruminantium (12 h) was lower than the wild-type (24 h). The maximum biomass and specific growth rate of mutant M. ruminantium were significantly lower (P < 0.05) than those of wild-type, and the maximum biomass of mutant M. ruminantium was approximately half of the wild-type; meanwhile, the proliferation was reduced. The synthesis amount of coenzyme F420 of M. ruminantium was significantly decreased (P < 0.05) after the cofD gene knock-out. Moreover, the maximum amount of H2 consumed and CH4 produced by mutant were 14 and 2% of wild-type M. ruminantium respectively. In conclusion, cofD gene knock-out induced the decreased growth rate and reproductive ability of M. ruminantium. Subsequently, the synthesis of coenzyme F420 was decreased. Ultimately, the production capacity of CH4 in M. ruminantium was reduced. Our research provides evidence that cofD gene plays an indispensable role in the regulation of coenzyme F420 synthesis and CH4 production in M. ruminantium.
Human pluripotent stem cell-derived brain organoids as in vitro models for studying neural disorders and cancer
Authors: Luo Juan , Li Peng
Published: May 2021

The sheer complexities of brain and resource limitation of human brain tissue greatly hamper our understanding of the brain disorders and cancers. Recently developed three-dimensional (3D) brain organoids (BOs) are self-organized and spontaneously differentiated from human pluripotent stem cells (hPSCs) in vitro, which exhibit similar features with cell type diversity, structural organization, and functional connectivity as the developing human brain. Based on these characteristics, hPSC-derived BOs (hPDBOs) provide new opportunities to recapitulate the complicated processes during brain development, neurodegenerative disorders, and brain cancers in vitro. In this review, we will provide an overview of existing BO models and summarize the applications of this technology in modeling the neural disorders and cancers. Furthermore, we will discuss the challenges associated with their use as in vitro models for disease modeling and the potential future direction.
Solving a well-posed fractional initial value problem by a complex approach
Authors: Fernandez Arran , Uçar Sümeyra , Özdemir Necati
Published: May 2021

Nonlinear fractional differential equations have been intensely studied using fixed point theorems on various different function spaces. Here we combine fixed point theory with complex analysis, considering spaces of analytic functions and the behaviour of complex powers. It is necessary to study carefully the initial value properties of Riemann–Liouville fractional derivatives in order to set up an appropriate initial value problem, since some such problems considered in the literature are not well-posed due to their initial conditions. The problem that emerges turns out to be dimensionally consistent in an unexpected way, and therefore suitable for applications too.
Household willingness to adopt a single-stage solar-supported hyper-thermophilic anaerobic biogas digester in Ghana
Authors: Bryant Isaac Mbir , Afitiri Abdul-Rahaman
Published: May 2021

Background

Sustainability of energy is key for quality life; thus, the use of clean energy at the household level warrants moving from fossil-based energy to modern forms like biogas. However, the joint interactive effect of household income, biogas usage and willingness to adopt a single-stage solar-supported hyper-thermophilic anaerobic biogas digester (SSHTABD) is not known.

Methods

A cross-sectional survey was carried out to assess the willingness of residents of Elmina to adopt the SSHTABD. Stratified and simple random sampling techniques were used to select 219 respondents fitted into a complementary log–log regression model.

Results

Household willingness to adopt the SSHTABD was 86%. Among them are households not willing to use biogas but have high income and households willing to use biogas but have either low or high income are more likely to adopt the technology compared to households not willing to use biogas and have low income. Households not willing to use biogas, but have high income (OR = 1.725, confidence interval [CI] 0.803–3.706) and households willing to use biogas, but have low income (OR = 1.877, CI 1.103–3.188) compared to households willing to use biogas and have high income (OR = 1.725, CI 1.080–3.451) are more likely to adopt the technology as households not willing to use biogas and have low income. Additionally, households employed under the formal government sector, formal and informal private sectors are 40%, 136% and 103%, respectively, more likely to adopt the technology than those unemployed.

Conclusion

The high willingness of households to adopt the technology calls for government to support households to own biogas digesters thus requires policy interventions and interdisciplinary research.
The emerging role of RNA N6-methyladenosine methylation in breast cancer
Authors: Zheng Fangchao , Du Feng , Zhao Jiuda , Wang Xue , Si Yiran , Jin Peng , Qian Haili , Xu Binghe , Yuan Peng
Published: May 2021

N6-methyladenosine (m6A) modification is the most prevalent internal mRNA modification and is involved in many biological processes in eukaryotes. Accumulating evidence has demonstrated that m6A may play either a promoting or suppressing role in breast cancer, including in tumorigenesis, metastasis and angiogenesis. In this review, we summarize the latest research progress on the biological function and prognostic value of m6A modification in breast cancer, as well as potential related therapeutic strategies.
Evolutionary divergence of motifs in B-class MADS-box proteins of seed plants
Authors: Shen Gangxu , Jia Yong , Wang Wei-Lung
Published: May 2021

Background

MADS-box transcription factors function as homo- or heterodimers and regulate many aspects of plant development; moreover, MADS-box genes have undergone extensive duplication and divergence. For example, the morphological diversity of floral organs is closely related to the functional divergence of the MADS-box gene family. B-class genes (such as Arabidopsis thaliana APETALA3 [AP3] and PISTILLATA [PI]) belong to a subgroup of MADS-box genes. Here, we collected 97 MADS-box B protein sequences from 21 seed plant species and examined their motifs to better understand the functional evolution of B proteins.

Results

We used the MEME tool to identify conserved sequence motifs in these B proteins; unique motif arrangements and sequences were identified in these B proteins. The keratin-like domains of Malus domestica and Populus trichocarpa B proteins differed from those in other angiosperms, suggesting that a novel regulatory network might have evolved in these species. The MADS domains of Nelumbo nucifera, Glycine max, and Amborella trichopoda B-proteins contained motif 9; in contrast, those of other plants contained motif 1. Protein modelling analyses revealed that MADS domains with motif 9 may lack amino acid sites required for DNA-binding. These results suggested that the three species might share an alternative mechanism controlling floral development.

Conclusions

Amborella trichopoda has B proteins with either motif 1 or motif 9 MADS domains, suggesting that these two types of MADS domains evolved from the ancestral domain into two groups, those with motif 9 (N. nucifera and G. max), and those with motif 1. Moreover, our results suggest that the homodimer/heterodimer intermediate transition structure first appeared in A. trichopoda. Therefore, our systematic analysis of the motifs in B proteins sheds light on the evolution of these important transcription factors.
Association between prevalence of obstructive lung disease and obesity: results from The Vermont Diabetes Information System
Authors: Ramos-Nino Maria E. , MacLean Charles D. , Littenberg Benjamin
Published: May 2021

Background

The association of obesity with the development of obstructive lung disease, namely asthma and/or chronic obstructive pulmonary disease, has been found to be significant in general population studies, and weight loss in the obese has proven beneficial in disease control. Obese patients seem to present with a specific obstructive lung disease phenotype including a reduced response to corticosteroids. Obesity is increasingly recognized as an important factor to document in obstructive lung disease patients and a critical comorbidity to report in diabetic patients, as it may influence disease management. This report presents data that contributes to establishing the relationship between obstructive lung disease in a diabetic cohort, a population highly susceptible to obesity.

Methods

A total of 1003 subjects in community practice settings were interviewed at home at the time of enrolment into the Vermont Diabetes Information System, a clinical decision support program. Patients self-reported their personal and clinical characteristics, including any history of obstructive lung disease. Laboratory data were obtained directly from the clinical laboratory, and current medications were obtained by direct observation of medication containers. We performed a cross-sectional analysis of the interviewed subjects to assess a possible association between obstructive lung disease history and obesity.

Results

In a multivariate logistic regression model, a history of obstructive lung disease was significantly associated with obesity (body mass index ≥30) even after correcting for potential confounders including gender, low income (<$30,000/year), number of comorbidities, number of prescription medications, cigarette smoking, and alcohol problems (adjusted odds ratio (OR) = 1.58, P = 0.03, 95% confidence interval (CI) = 1.05, 2.37). This association was particularly strong and significant among female patients (OR = 2.18, P = < 0.01, CI = 1.27, 3.72) but not in male patients (OR = 0.97, P = 0.93, CI = 0.51, 1.83).

Conclusion

These data suggest an association between obesity and obstructive lung disease prevalence in patients with diabetes, with women exhibiting a stronger association. Future studies are needed to identify the mechanism by which women disproportionately develop obstructive lung disease in this population.
Functional hemispherectomy for patients with adult-onset Rasmussen’s encephalitis
Authors: Xue Hong , Xu Cuiping , Zhou Xiaoxia , Ni Duanyu , Wang Xueyuan , Yu Tao
Published: May 2021

Background

Surgical treatment for patients with adult-onset Rasmussen’s encephalitis (A-RE) is rarely reported. We investigated the clinical and surgical features of two patients with A-RE who underwent functional hemispherectomy.

Case presentation

The data of clinical manifestations, neuroimaging, surgical treatment and surgical outcomes of two patients with A-RE was reviewed. The two patients initially presented with recurrent partial seizures or secondly generalized tonic clonic seizures. Gradually, the patients showed unilateral limb paralysis as well as chronic focal epileptic status. Both patients underwent functional hemispherectomy and achieved seizure freedom in the follow-up. The contralateral neurological deficits improved gradually after rehabilitation and were acceptable for the self-care of daily living. The living quality improved prominently after surgery.

Conclusions

Despite the risk of hemiplegia, functional hemispherectomy may be a choice for patients with A-RE for favorable seizure control and improved quality of life in selected patients.
Multivariate optimization and evaluation of quaternary mixture in bulk and co-formulated dosage forms by central composite design
Authors: Jampana Rama Tulasi , Avula Prameela Rani , Anumolu Panikumar Durga
Published: May 2021

Background

The current study describes the use of central composite design for multivariate optimization of resolution and retention time, taking into account different critical method parameters like organic phase, pH, flow rate, and wavelength for risk assessment. The chromatographic method for the assay of the most effective anti-viral regimen (EPCLUSA, DARVONI, and HARVONI) was developed. An experimental design was presented by sequential investigation of four independent parameters. The method was developed using XTERRA C18 (250 mm × 4.6 mm, 5 μm particle size) column in isocratic mode using potassium dihydrogen phosphate buffer (pH adjusted to 5) and acetonitrile (50:50 % v/v) as mobile phase at a flow rate of 1.0 ml/min and UV detection wavelength of 260 nm.

Results

The separation of four drugs with fine resolution and preferable retention times was achieved. Retention times of four drugs were found to be 2.96, 3.91, 7.15, and 11.94 min for daclatasvir, sofosbuvir, velpatasvir, and ledipasvir, respectively. The percentage accuracy of labelled claim was in the range of 99–102%, and the pooled %RSD for repeatability, precision, and accuracy was less than 2%.

Conclusion

The suggested method was applied for quantification and identification of studied drugs in tablets; the results agreed with the label claim and were validated according to the ICH guidelines. The optimized method can be used for pharmacokinetic and quality control studies.
A method for adequate selection of training data sets to reconstruct seismic data using a convolutional U-Net
Authors: Park Jiho , Choi Jihun , Jee Seol Soon , Byun Joongmoo , Kim Young
Published: May 2021

Deep-learning (DL) methods have recently been introduced for seismic signal processing. Using DL methods, many researchers have adopted these novel techniques in an attempt to construct a DL model for seismic data reconstruction. The performance of DL-based methods depends heavily on what is learned from the training data. We focus on constructing the DL model that well reflect the features of target data sets. The main goal is to integrate DL with an intuitive data analysis approach that compares similar patterns prior to the DL training stage. We have developed a two-sequential method consisting of two stages: (1) analyzing training and target data sets simultaneously for determining the target-informed training set and (2) training the DL model with this training data set to effectively interpolate the seismic data. Here, we introduce the convolutional autoencoder t-distributed stochastic neighbor embedding (CAE t-SNE) analysis that can provide the insight into the results of interpolation through the analysis of training and target data sets prior to DL model training. Our method was tested with synthetic and field data. Dense seismic gathers (e.g., common-shot gathers) were used as a labeled training data set, and relatively sparse seismic gathers (e.g., common-receiver gathers [CRGs]) were reconstructed in both cases. The reconstructed results and signal-to-noise ratios demonstrated that the training data can be efficiently selected using CAE t-SNE analysis, and the spatial aliasing of CRGs was successfully alleviated by the trained DL model with this training data, which contain target features. These results imply that the data analysis for selecting target-informed training set is very important for successful DL interpolation. In addition, our analysis method can also be applied to investigate the similarities between training and target data sets for other DL-based seismic data reconstruction tasks.
Seismic inversion via closed-loop fully convolutional residual network and transfer learning
Authors: Wang Lingling , Meng Delin , Wu Bangyu
Published: May 2021

Because deep-learning networks can “learn” the complex mapping function between labeled inputs and outputs, they have shown great potential in seismic inversion. Conventional deep-learning algorithms require a large amount of labeled data for sufficient training. However, in practice, the number of well logs is limited. To address this problem, we have adopted a closed-loop fully convolutional residual network (FCRN) combined with a transfer learning strategy for seismic inversion. This closed-loop FCRN consists of an inverse network and a forward network. The inverse network predicts the inversion target from seismic data, whereas the forward network calculates seismic data from the inversion target. The inverse network is initialized by pretraining on the Marmousi2 model and is fine-tuned with the limited labeled data around the wells through transfer learning, to suit the target seismic data. The forward network is initialized by training with the limited labeled data around the wells. In this way, the closed-loop network is well initialized to ensure relatively good convergence. Then, the misfit of the limited labeled data and the error between the true and the forward seismic data are used to regularize the training of the initialized closed-loop network. The inverse network of the optimized closed-loop network is used to obtain the final inversion results. Our workflow can be used for velocity, density, and impedance inversion from poststack seismic data. We take velocity inversion as an example to illustrate the effectiveness of the method. The experimental results show that closed-loop FCRN with transfer learning is superior to open-loop FCRN with better lateral continuity and velocity details. Closed-loop FCRN can effectively predict velocity with high accuracy on synthetic data, has good antinoise performance, and can also be effectively used for field data with spatial heterogeneity.
Phenomenological models for estimating and constraining c13 for transversely isotropic hydrocarbon source rocks
Authors: Yan Fuyong , Vernik Lev
Published: May 2021

Hydrocarbon source rocks can be adequately approximated as transversely isotropic (TI) media. The elastic properties of a TI medium are defined by five stiffness parameters: [Formula: see text], [Formula: see text], [Formula: see text], [Formula: see text], and [Formula: see text]. The laboratory estimation of [Formula: see text], [Formula: see text], [Formula: see text], and [Formula: see text] is straightforward, with each of the stiffness parameters determined by a single velocity measurement in an orthogonal direction. For [Formula: see text], we need the information of [Formula: see text], [Formula: see text], [Formula: see text], and at least one oblique velocity. Consequently, it is usually more difficult to estimate [Formula: see text] than the other stiffness parameters in the laboratory, and it is even more challenging to acquire it from the field. Therefore, it is important to find the relations between [Formula: see text] and the other stiffness parameters so that [Formula: see text] can be estimated from the orthogonal elastic tensor elements [Formula: see text], [Formula: see text], [Formula: see text], and [Formula: see text] that can be much more economically and reliably acquired. There are phenomenological models for estimating [Formula: see text] from the other stiffness parameters, but their accuracy is not always satisfactory. We find that [Formula: see text] has strong correlations with [Formula: see text] and [Formula: see text], and that [Formula: see text] generally underestimates [Formula: see text] and [Formula: see text] generally overestimates [Formula: see text]. The average of [Formula: see text] and [Formula: see text] can be a much more precise phenomenological model to approximate [Formula: see text]. We also find that [Formula: see text] is generally greater than [Formula: see text] for hydrocarbon source rocks. Therefore, we determine that [Formula: see text] should lie between [Formula: see text] and [Formula: see text] for hydrocarbon source rocks.
Subbasalt Marchenko imaging with offshore Brazil field data
Authors: Jia Xueyi , Baumstein Anatoly , Jing Charlie , Neumann Erik , Snieder Roel
Published: May 2021

Subbasalt imaging for hydrocarbon exploration faces challenges with the presence of multiple scattering, attenuation, and mode conversion as seismic waves encounter highly heterogeneous and rugose basalt layers. A combination of modern seismic acquisition that can record densely sampled data and advanced imaging techniques make imaging through basalt feasible. Yet, the internal multiples, if not properly handled during seismic processing, can be mapped to reservoir layers by conventional imaging methods, misguiding geologic interpretation. Traditional internal multiple elimination methods suffer from the requirement of picking horizons of multiple generators and/or a top-down adaptive subtraction process. Marchenko imaging provides an alternative solution to directly remove the artifacts due to internal multiples, without the need of horizon picking or subtraction. In this paper, we evaluate a successful application of direct Marchenko imaging for subbasalt demultiple and imaging with an offshore Brazil field data set. The internal multiples in this example are generated from the seabed and basalt layers, causing severe artifacts in conventional seismic images. We have determined that these artifacts are largely suppressed with Marchenko imaging, and we have developed a general workflow for data preprocessing and regularization of marine streamer data sets. We find that horizontally propagating waves also can be reconstructed by the Marchenko method at far offsets.
Gemtuzumab Ozogamicin Improves Event-Free Survival and Reduces Relapse in PediatricKMT2A-Rearranged AML: Results From the Phase III Children's Oncology Group Trial AAML0531
Authors: Pollard Jessica A. , Guest Erin , Alonzo Todd A. , Gerbing Robert B. , Loken Mike R. , Brodersen Lisa Eidenschink , Kolb E. Anders , Aplenc Richard , Meshinchi Soheil , Raimondi Susana C. , Hirsch Betsy , Gamis Alan S.
Published: May 2021

PURPOSE

We investigated the impact of the CD33-targeted agent gemtuzumab ozogamicin (GO) on survival in pediatric patients with KMT2A-rearranged ( KMT2A-r) acute myeloid leukemia (AML) enrolled in the Children's Oncology Group trial AAML0531 ( NCT01407757 ).

METHODS

Patients with KMT2A-r AML were identified and clinical characteristics described. Five-year overall survival (OS), event-free survival (EFS), disease-free survival (DFS), and relapse risk (RR) were determined overall and for higher-risk versus not high-risk translocation partners. GO's impact on response was determined and outcomes based on consolidation approach (hematopoietic stem cell transplant [HSCT] v chemotherapy) described.

RESULTS

Two hundred fifteen (21%) of 1,022 patients enrolled had KMT2A-r AML. Five-year EFS and OS from study entry were 38% and 58%, respectively. EFS was superior with GO treatment (EFS 48% with GO v 29% without, P = .003), although OS was comparable (63% v 53%, P = .054). For patients with KMT2A-r AML who achieved complete remission, GO was associated with lower RR (40% GO v 66% patients who did not receive GO [No-GO], P = .001) and improved 5-year DFS (GO 57% v No-GO 33%, P = .002). GO benefit was observed in both higher-risk and not high-risk KMT2A-r subsets. For patients who underwent HSCT, prior GO exposure was associated with decreased relapse (5-year RR: 28% GO and HSCT v 73% No-GO and HSCT, P = .006). In multivariable analysis, GO was independently associated with improved EFS, improved DFS, and reduced RR.

CONCLUSION

GO added to conventional chemotherapy improved outcomes for KMT2A-r AML; consolidation with HSCT may further enhance outcomes. Future clinical trials should study CD33-targeted agents in combination with HSCT for pediatric KMT2A-r AML.
Sensory Neurons, Neuroimmunity, and Pain Modulation by Sex Hormones
Authors: Lenert Melissa E , Avona Amanda , Garner Katherine M , Barron Luz R , Burton Michael D
Published: May 2021

The inclusion of women in preclinical pain studies has become more commonplace in the last decade as the National Institutes of Health (NIH) released its “Sex as a Biological Variable” mandate. Presumably, basic researchers have not had a comprehensive understanding about neuroimmune interactions in half of the population and how hormones play a role in this. To date, we have learned that sex hormones contribute to sexual differentiation of the nervous system and sex differences in behavior throughout the lifespan; however, the cycling of sex hormones does not always explain these differences. Here, we highlight recent advances in our understanding of sex differences and how hormones and immune interactions influence sensory neuron activity to contribute to physiology and pain. Neuroimmune mechanisms may be mediated by different cell types in each sex, as the actions of immune cells are sexually dimorphic. Unfortunately, the majority of studies assessing neuronal contributions to immune function have been limited to males, so it is unclear if the mechanisms are similar in females. Finally, pathways that control cellular metabolism, like nuclear receptors, have been shown to play a regulatory role both in pain and inflammation. Overall, communication between the neuroimmune and endocrine systems modulate pain signaling in a sex-dependent manner, but more research is needed to reveal nuances of these mechanisms.
Acute Migraine Treatment
Authors: Ailani Jessica
Published: May 2021

PURPOSE OF REVIEW

Migraine is a disabling disease of attacks of moderate to severe pain with associated symptoms. Every person with migraine requires treatment for acute attacks. Treatments can range from behavioral management and nonspecific medications to migraine-specific medications and neuromodulation. For many with migraine, having a combination of tools allows for effective treatment of all types of attacks.

RECENT FINDINGS

Over the past several years, four neuromodulation devices have been cleared by the US Food and Drug Administration (FDA) for treatment of acute migraine, and three medications with novel mechanisms of action have been FDA approved. They add to the arsenal available to people with migraine and focus on migraine-specific pathways to allow for precise care with fewer side effects.

This article discusses acute migraine therapy, focusing on best-level evidence.