bluuebunny/crossref_metadata_2025 huggingface dataset:
Validating the use of clinical MSCT scans for cranial nonmetric sex estimation in a contemporary Indonesian population
Authors: Lye Ridhwan , Obertová Zuzana , Bachtiar Nur Amelia , Franklin Daniel
Published: February 2024

There is renewed interest in Asia for the development of forensic anthropological standards, partly due to the need for population-specific models to maintain high classification accuracies. At present, there are no known studies utilising morphoscopic standards specific to the Indonesian population. Craniometric analyses can often be time-consuming; morphoscopic assessments are quicker and are also known to be reliable and accurate. One of the most utilised morphoscopic standards for the estimation of skeletal sex is that of Walker (2008). Its application across population groups demonstrated reduced accuracies outside of the United States; population-specific predictive models would thus serve to improve the identification process of unknown skeletal remains. Digital imaging also allows for the validation of standards on a contemporary population and is an appropriate proxy to physical skeletal material.

The present study quantifies the applicability of the Walker standard to a contemporary Indonesian population. A total of 200 cranial MSCT scans from a hospital in Makassar were analysed using OsiriX®. Scoring was performed in accordance with the Walker standard. Five univariate and nine multivariate predictive models were derived using single trait and multi-trait combinations. The best performing univariate model included the glabella, with a total classification accuracy of 82.0% and a sex bias of 14.6%. Classification accuracy with all traits considered was at 95.2% for females and 82.8% for males with a sex bias of 12.5%. These results provide forensic practitioners in Indonesia with an appropriate morphoscopic sex estimation standard, strengthening their capabilities in the field and improving judicial outcomes.
Association of triglyceride‑glucose index in branch retinal vein occlusion
Authors: Aslan Sirakaya Hatice , Sirakaya Ender
Published: February 2024

Background

To investigate the association between the triglyceride-glucose (TyG) index and newly diagnosed branch retinal vein occlusion (BRVO) in patients.

Methods

The study included 57 individuals with BRVO and a control group comprising 50 healthy volunteers matched for age and gender. Detailed eye examinations were conducted, and various blood biochemistry and hematological parameters were recorded. The TyG index was calculated using fasting plasma glucose and triglyceride values.

Results

The mean age was 61.4 ± 9.6 years for the BRVO group and 60.6 ± 10.3 years for the control group. The TyG values were significantly higher in the BRVO group when compared to the control group (8.84 ± 0.41 vs. 8.52 ± 0.29, p < 0.001). Multivariate analysis revealed that the TyG index independently predicted BRVO (odds ratio = 2.58, 95% confidence interval = 1.69–3.93; p < 0.001). In receiver operating characteristics analysis, the TyG index had an area under the curve of 0.749, and a TyG index higher than 8.52 predicted BRVO with 83% sensitivity and 70% specificity.

Conclusions

This study establishes a significant association between an elevated TyG index and BRVO. Consequently, the TyG index could serve as a valuable predictive tool for identifying individuals at risk for BRVO.
Short term e-bicycle riding results in favorable cardiometabolic shifts in moderately active adults
Authors: Alessio Helaine M. , Ballard Kevin D. , Reidy Paul T. , Hayward Katie M. , Bagg Alexandra M. , Cooley Rachel A. , O’Connell Michael J. , Montoye Alexander H. K. , Timmerman Kyle L.
Published: February 2024

Purpose

Electric bikes (EB) are a form of active transportation with demonstrated health benefits. The purpose of this study was to determine the influence of riding an EB for one week on indices of cardiometabolic health in middle-aged adults.

Methods

Adults (n = 22; age = 57.1 ± 11.3 year; BMI = 27.7 ± 4.9) participated in a 2 week study. During Week 1, participants were instructed to continue regular activities. Starting Week 2 participants were provided an EB to ride at least 3 days for a minimum of 30 min·day−1. Physical activity (PA) and glucose were measured continuously. Body composition, blood lipids, glucose, insulin, hemoglobin A1c (HbA1c), plasma endothelin-1 (ET-1), and carotid-femoral pulse wave velocity (cf-PWV) were measured on days 1 and 14.Data and Statistical analyses or Statistics. Each participant served as their own control. Paired t-tests compared dependent variables between week 1 (without EB) and week 2 (with EB).

Results

When provided an EB for one week, moderate to vigorous PA increased by 6–9 min·day−1 (P < 0.05) and sedentary time decreased by ~ 77 min·day−1 (P < 0.05). Data from 24 h continuous glucose monitoring showed the percentage of time in healthy range (70–120 mg·dl−1 glucose) increased (P < 0.05) from week 1 to week 2. Compared to day 1, cf-PWV was lower at day 14 (P < 0.05) following one week of riding an EB.

Conclusion

Moderately-active, middleaged adults showed improved continuous glucose regulation and lower central arterial stiffness following one week of riding an EB.
Is the European badger a new host for Dirofilaria immitis? The first records in Greece
Authors: Markakis Grigorios , Sioutas Georgios , Bitchava Dimitra , Komnenou Anastasia , Ganoti Maria , Papadopoulos Elias
Published: February 2024

Dirofilaria immitis is a ubiquitous nematode parasite with zoonotic potential, transmitted by mosquitoes, that causes heartworm disease in various animal species. Dogs are the parasite’s typical final host, and wild carnivores represent the parasite’s reservoir in nature. Studies on D. immitis infections in wild animals are essential to assess infection pressure for domestic animals, and until now, there has been only one infection case reported in a European badger (Meles meles). The current report describes the first two European badger cases with cardiovascular dirofilariosis in Greece. Two adult male badgers were rescued in Heraklion and Chania, Crete Island, and admitted to “ANIMA -Wildlife Rehabilitation Centre” in Athens. The detailed clinical examination revealed that the first badger suffered from severe broncho-pneumonitis while the second one displayed clinical signs associated with severe brain trauma. Blood samples were taken for haematology and biochemistry analyses during their short hospitalisation period. In addition, different routine diagnostic tests were carried out, including heartworm antigen testing (ELISA) and the modified Knott’s test for microfilariae. Both badgers were positive in both tests. The animals died a few hours after their admission and the detailed necropsies followed, revealed the presence of three parasites in each animal’s right heart, morphologically identified as adults of D. immitis. These findings add the European badger in the list of additional potential reservoir hosts for D. immitis and highlight the potential role of wildlife for companion animals and human health.
Whole exome sequencing and polygenic assessment of a Swedish cohort with severe developmental language disorder
Authors: Yahia Ashraf , Li Danyang , Lejerkrans Sanna , Rajagopalan Shyam , Kalnak Nelli , Tammimies Kristiina
Published: February 2024

Developmental language disorder (DLD) overlaps clinically, genetically, and pathologically with other neurodevelopmental disorders (NDD), corroborating the concept of the NDD continuum. There is a lack of studies to understand the whole genetic spectrum in individuals with DLD. Previously, we recruited 61 probands with severe DLD from 59 families and examined 59 of them and their families using microarray genotyping with a 6.8% diagnostic yield. Herein, we investigated 53 of those probands using whole exome sequencing (WES). Additionally, we used polygenic risk scores (PRS) to understand the within family enrichment of neurodevelopmental difficulties and examine the associations between the results of language-related tests in the probands and language-related PRS. We identified clinically significant variants in four probands, resulting in a 7.5% (4/53) molecular diagnostic yield. Those variants were in PAK2, MED13, PLCB4, and TNRC6B. We also prioritized additional variants for future studies for their role in DLD, including high-impact variants in PARD3 and DIP2C. PRS did not explain the aggregation of neurodevelopmental difficulties in these families. We did not detect significant associations between the language-related tests and language-related PRS. Our results support using WES as the first-tier genetic test for DLD as it can identify monogenic DLD forms. Large-scale sequencing studies for DLD are needed to identify new genes and investigate the polygenic contribution to the condition.
Amino acid distribution in blood following high-intensity interval exercise: a preliminary study
Authors: Borges Nattai , Doering Thomas M. , Murphy Grace , Macdonald Margaret , Dunstan Richard H.
Published: February 2024

This study investigated the effect of high-intensity interval exercise on total and individual amino acid concentrations in red blood cells (RBCs) and plasma. Seven males (31 ± 13 yr) provided venous blood samples at rest, immediately and 15 min and 30 min following an 8-min high-intensity exercise bout. The exercise bout was 16 × 15 s cycle efforts at 0.4N/kg of body mass and 90 rpm, interspersed with 15 s passive recovery. Total and individual amino acid concentrations of RBC and plasma and blood cell parameters were analysed. No significant differences for total amino acid concentrations between RBC and plasma were found. Individual amino acid analyses showed significant interaction effects for alanine and α-aminoadipic acid (P < 0.05), with plasma alanine significantly increased from baseline across the recovery period (P < 0.001). Blood fraction (group) effects showed greater concentrations of glycine, serine, asparagine, aspartic acid, glutamic acid, α-aminoadipic acid and ornithine in RBC, while greater concentrations of alanine, α-aminobutyric acid, valine, leucine, isoleucine, threonine, proline, phenylalanine, glutamine, tryptophan and cystine were found in plasma (P < 0.05). Comparable levels of histidine, lysine and tyrosine were observed between blood fractions. Significant differences in the variation of total amino acids in RBC were reported with higher variance at rest compared to following exercise (P = 0.01). Haemoglobin, pack cell volume and white blood cell count significantly increased immediately following exercise (P < 0.05) but returned to baseline after 15 min recovery. These results support the notion of individualised amino acid transportation roles for RBC and plasma during exercise.
Peer relationships, adolescent anxiety, and life satisfaction: a moderated mediation model in Turkish and syrian samples
Authors: Yetim Onat , Çakır Resul , Bülbül Ece , Alleil İlham Sebea
Published: February 2024

Prior studies comparing Syrian refugee adolescents to their native peers in the same region have found higher anxiety and lower life satisfaction. Therefore, identifying regulatory variables is crucial for implementing support programs. This study examined the mediating effect of peer relationships and the moderating effect of being a refugee or native adolescent on the relationship between adolescent anxiety and life satisfaction across different samples. Participants and setting: The study included 2,336 adolescents aged 11–19 (M = 14.79, SD = 1.04). Participants completed the Screen for Child Anxiety Related Disorders, Satisfaction with Life Scale, and Strengths and Difficulties Questionnaire. The mediation and moderation effects were analyzed with the path analysis codes written on Mplus 8.3. SPSS 26 was used for descriptive statistics and group comparisons. The findings showed that peer relationships mediate adolescent anxiety and life satisfaction, and this relationship is moderated according to whether the participants are native adolescents or refugee adolescents. This study highlights the significant associations between peer relationships, adolescent anxiety, and life satisfaction and the moderating role of the participant identity. The findings may inform psychological interventions to improve Syrian refugee adolescents' mental health and well-being. These findings may also have implications for policies and programs aimed at supporting the integration of Syrian refugee adolescents in host communities.
Interfacial structure changes between amorphous silicon anode/liquid electrolyte using a highly dense and flat model electrode
Authors: Asano Sho , Hata Jun-ichi , Watanabe Kenta , Matsui Naoki , Suzuki Kota , Kanno Ryoji , Hirayama Masaaki
Published: February 2024

Changes in the interfacial structures of the amorphous silicon (a-Si) anode/organic electrolyte interfaces in lithium-ion batteries were investigated using highly dense a-Si films fabricated by cathodic arc plasma deposition as a model electrode. Raman spectroscopy, transmission electron microscopy (TEM), and X-ray reflectivity revealed that the Si films were grown in an amorphous state with an atomically flat surface. The a-Si films exhibited lithium alloying and de-alloying reactions, with a large irreversible capacity during the first cycle. The irreversible capacity was derived from the formation of a solid electrolyte interphase (SEI) along with an incompletely de-alloyed Li-Si phase, as confirmed by ex situ TEM and X-ray photoelectron spectroscopy observations. The discharge and charge capacities of the Si films gradually decreased in the subsequent cycles, despite the fact that no further SEI formation or cracking of the Si layer occurred. Scanning electron microscopy observations combined with energy-dispersive X-ray spectroscopy revealed the miniaturization of the a-Si film in the surface region to generate domains measuring less than a few hundred nanometers. These results suggest that delamination and miniaturization of the a-Si nanodomains from the electrode surface are partly responsible for degradation of the a-Si anode.
Impact of biologically effective dose on tremor decrease after stereotactic radiosurgical thalamotomy for essential tremor: a retrospective longitudinal analysis
Authors: Tuleasca Constantin , Carey Guillaume , Barriol Romain , Touzet Gustavo , Dubus Francois , Luc Defebvre , Carriere Nicolas , Reyns Nicolas
Published: February 2024

Stereotactic radiosurgery (SRS) is one of the surgical alternatives for drug-resistant essential tremor (ET). Here, we aimed at evaluating whether biologically effective dose (BEDGy2.47) is relevant for tremor improvement after stereotactic radiosurgical thalamotomy in a population of patients treated with one (unplugged) isocenter and a uniform dose of 130 Gy. This is a retrospective longitudinal single center study. Seventy-eight consecutive patients were clinically analyzed. Mean age was 69.1 years (median 71, range 36–88). Mean follow-up period was 14 months (median 12, 3–36). Tremor improvement was assessed at 12 months after SRS using the ET rating assessment scale (TETRAS, continuous outcome) and binary (binary outcome). BED was defined for an alpha/beta of 2.47, based upon previous studies considering such a value for the normal brain. Mean BED was 4573.1 Gy2.47 (median 4612, 4022.1–4944.7). Mean beam-on time was 64.7 min (median 61.4; 46.8–98.5). There was a statically significant correlation between delta (follow-up minus baseline) in TETRAS (total) with BED (p = 0.04; beta coefficient − 0.029) and beam-on time (p = 0.03; beta coefficient 0.57) but also between TETRAS (ADL) with BED (p = 0.02; beta coefficient 0.038) and beam-on time (p = 0.01; beta coefficient 0.71). Fractional polynomial multivariate regression suggested that a BED > 4600 Gy2.47 and a beam-on time > 70 min did not further increase clinical efficacy (binary outcome). Adverse radiation events (ARE) were defined as larger MR signature on 1-year follow-up MRI and were present in 7 out of 78 (8.9%) cases, receiving a mean BED of 4650 Gy2.47 (median 4650, range 4466–4894). They were clinically relevant with transient hemiparesis in 5 (6.4%) patients, all with BED values higher than 4500 Gy2.47. Tremor improvement was correlated with BED Gy2.47 after SRS for drug-resistant ET. An optimal BED value for tremor improvement was 4300–4500 Gy2.47. ARE appeared for a BED of more than 4500 Gy2.47. Such finding should be validated in larger cohorts.
A content analysis of alcohol imagery in season 2 of The Kardashians
Authors: Barker Alexander B. , Scott Charlotte L. , Sharpe Emma E.
Published: February 2024

Aim

The aim of the current study was to quantify the amount of alcohol content and branding in season 2 of the reality TV show The Kardashians.

Subject and methods

Content analysis of alcohol content in all 10 episodes of season 2 of The Kardashians was undertaken, and alcohol content measured using 1-minute interval coding. The presence of alcohol in the following four categories was noted: (1) actual use, (2) implied use, (3) other alcohol reference, and (4) brand appearance.

Results

Alcohol content was seen across all 10 episodes. Actual alcohol use was seen in six episodes across 13 intervals. Implied alcohol use was seen in all 10 episodes across 74 intervals. Alcohol branding was seen in six episodes, across 23 intervals. The Tequila brand 818, owned by Kendall Jenner, was seen 65 times across 17 intervals.

Conclusion

The current study shows that alcohol content was highly prevalent in season 2 of The Kardashians. This programme was popular on the Disney+ streaming platform and likely resulted in widespread population exposure to alcohol content and branding, particularly to a brand owned by Kendall Jenner. Current rules and regulations are not sufficient to protect against exposure to this potentially harmful content.
Does Engagement in HIV Care Affect Screening, Diagnosis, and Control of Noncommunicable Diseases in Sub-Saharan Africa? A Systematic Review and Meta-analysis
Authors: Kileel Emma M. , Zheng Amy , Bor Jacob , Fox Matthew P. , Crowther Nigel J. , George Jaya A. , Khoza Siyabonga , Rosen Sydney , Venter Willem D. F. , Raal Frederick , Hibberd Patricia , Brennan Alana T.
Published: February 2024

Low- and middle-income countries are facing a growing burden of noncommunicable diseases (NCDs). Providing HIV treatment may provide opportunities to increase access to NCD services in under-resourced environments. We conducted a systematic review and meta-analysis to evaluate whether use of antiretroviral therapy (ART) was associated with increased screening, diagnosis, treatment, and control of diabetes, hypertension, chronic kidney disease, or cardiovascular disease among people living with HIV in sub-Saharan Africa (SSA). A comprehensive search of electronic literature databases for studies published between 01 January 2011 and 31 December 2022 yielded 26 studies, describing 13,570 PLWH in SSA, 61% of whom were receiving ART. Random effects models were used to calculate summary odds ratios (ORs) of the risk of diagnosis by ART status and corresponding 95% confidence intervals (95% CIs), where appropriate. ART use was associated with a small but imprecise increase in the odds of diabetes diagnosis (OR 1.07; 95% CI 0.71, 1.60) and an increase in the odds of hypertension diagnosis (OR 2.10, 95% CI 1.42, 3.09). We found minimal data on the association between ART use and screening, treatment, or control of NCDs. Despite a potentially higher NCD risk among PLWH and regional efforts to integrate NCD and HIV care, evidence to support effective care integration models is lacking.
Local-global methods for generalised solar irradiance forecasting
Authors: Cargan Timothy R. , Landa-Silva Dario , Triguero Isaac
Published: February 2024

For efficient operation, solar power operators often require generation forecasts for multiple sites with varying data availability. Many proposed methods for forecasting solar irradiance / solar power production formulate the problem as a time-series, using current observations to generate forecasts. This necessitates a real-time data stream and enough historical observations at every location for these methods to be deployed. In this paper, we propose the use of Global methods to train generalised models. Using data from 20 locations distributed throughout the UK, we show that it is possible to learn models without access to data for all locations, enabling them to generate forecasts for unseen locations. We show a single Global model trained on multiple locations can produce more consistent and accurate results across locations. Furthermore, by leveraging weather observations and measurements from other locations we show it is possible to create models capable of accurately forecasting irradiance at locations without any real-time data. We apply our approaches to both classical and state-of-the-art Machine Learning methods, including a Transformer architecture. We compare models using satellite imagery or point observations (temperature, pressure, etc.) as weather data. These methods could facilitate planning and optimisation for both newly deployed solar farms and domestic installations from the moment they come online.
Shifting Norms and Value Conflicts: Exploring the Effects of HIV Status Disclosure Fields in Sex-Social Apps
Authors: Warner Mark , Gibbs Jo , Blandford Ann
Published: February 2024

Sex-social applications used by men who have sex with men (MSM) often provide options to disclose HIV status to encourage more positive language and reduce stigma. Yet, little research has sought to understand how in-app disclosure fields impact on disclosure motivation. We interviewed MSM living with HIV and those who self-reported being HIV-negative ($$N=27$$

N
=
27

) in the UK and applied a hierarchical model of motivation to interpret our data. We found conflicting motivations for disclosure and point to HIV status disclosure fields having shifted disclosure norms, limiting their perceived optionality. Moreover, the pairwise and location-aware nature of these apps fails to support narrative forms of disclosure, reducing motivation. We highlight an opportunity to support users in disclosing by linking apps more explicitly to the social narratives developed through public health campaigns. This could reduce the required effort to explain “the science" behind different treatment and prevention options and promote a more consistent narrative.
Auditory Cues Alter the Magnitude and Valence of Subjective Sexual Arousal and Desire Induced by an Erotic Video
Authors: Pfaus James G. , Zakreski Ellen
Published: February 2024

Although women and men rate their subjective arousal similarly in response to “female-centric” erotic videos, women rate their subjective arousal lower than men in response to “male-centric” videos, which often end with the male’s ejaculation. This study asked whether ratings of subjective sexual arousal and desire using the Sexual Arousal and Desire Inventory (SADI) would be altered if this ending was present or absent, and whether including or excluding the accompanying soundtrack would influence the magnitude and direction of the responses. A total of 119 cis-gendered heterosexual undergraduates (59 women and 60 men) viewed an 11-min sexually explicit heterosexual video that ended with a 15-s ejaculation scene. Two versions of the video were created, one with the ejaculatory ending (E+) and one without (E−). Participants were assigned randomly to view one of the two versions with (S+) or without (S−) the accompanying soundtrack, after which they completed the state version of the SADI. Women and men found both sequences without sound less arousing on the Evaluative, Motivational, and Physiological subscales of the SADI relative to the S+ sequences. However, on the Negative/Aversive subscale, women found the E + S- sequence more negative than did men, whereas this difference was not found with sound. Thus, women and men were sensitive to the auditory content of sexually explicit videos, and scenes of sexual intercourse ending with explicit ejaculation increased the Evaluative and Motivational properties of subjective sexual arousal and desire. However, this occurred in women only when the auditory cues signaled a clear and gratifying sexual interaction.
Novel Insights into Obesity in Preschool Children with Autism Spectrum Disorder
Authors: van der Lubbe Anna , Swaab Hanna , Vermeiren Robert , van den Akker Erica , Ester Wietske
Published: February 2024

Obesity is present in 8–32% of the children with Autism Spectrum Disorder (ASD). However, most studies are performed in school-aged children from the USA. The current study compares obesity rates of Dutch preschoolers with ASD with children from the Dutch general population and explores which child- and parental factors are related to obesity in children with ASD. This cross-sectional study is part of the ongoing Tandem Study (Dutch Trial register: NL7534). Seventy-eight children with ASD aged 3–7 years and their parents (77 mothers, 67 fathers) participated. Child factors are: Body Mass Index (by physical measurement), child eating behavior (Child Eating Behavior Questionnaire), child problem behavior (Child Behavior Checklist), and ASD severity (Autism Diagnostic Observation Scale 2). Parental factors are: BMI (by physical measurement), parental eating behavior (Dutch Eating Behavior Inventory), parenting stress (The Parenting Stress Questionnaire) and highest completed educational level (SES). Children with ASD were 8 times more often obese (16.8%) than children from the general population (2.0%). Child BMI correlated positively with child food approach behavior and maternal BMI, and correlated negatively with child ‘Slowness in eating’. There was no correlation between child BMI and ASD severity, problem behavior, parental eating behavior, parental stress and SES. Thus, Dutch, preschool children with ASD have 8 times higher obesity rates than children from the general population. More attention to obesity risk in research and clinical care could contribute to the quality of life of individuals with ASD and their families.

Clinical Trial Registration

Dutch Trial register, NL7534, https://trialsearch.who.int/Trial2.aspx?TrialID=NL7534.
Perceptions of environmental changes among a climate-vulnerable population from Bangladesh
Authors: Freihardt Jan
Published: February 2024

Effective climate change adaptation requires a thorough understanding of whether and how affected populations perceive climatic and environmental changes. Existing research has been inconclusive regarding the consistency of these perceptions compared to objective meteorological indicators. Moreover, no systematic comparison has been done for the perception of discrete environmental events such as floods or erosion. This study relies on novel panel survey data of approximately 1700 households residing along the Jamuna River in Bangladesh as well as on unique individual-level, satellite-based erosion data. It compares respondents’ perceptions of environmental events, namely riverbank erosion, and three climate change indicators, specifically long-term temperature change and changes in precipitation during wet and dry seasons, to objective measurements using satellite imagery and climatic time-series data (CRU TS). I find that long-term temperature change is perceived more accurately than long-term changes in precipitation. Given that educational attainment and climate change literacy among the study population are low, this indicates that global temperature increases are felt even by remote populations who have never heard the term climate change. Erosion is strongly overestimated, especially by those respondents who had been personally affected by it. Since human behavior is guided by perceptions rather than objective data, this has important policy implications, underlining the importance of considering people’s perceptions if the goal is to assist them in adapting to environmental changes.
Associations of long non-coding RNAs HOTAIR, LINC00951, POLR2E and HULC polymorphisms with the risk of esophageal and esophagogastric junction cancer in a western population: a case-control study
Authors: Baili Efstratia , Gazouli Maria , Lazaris Andreas C. , Kanavidis Prodromos , Boura Maria , Michalinos Adamantios , Charalabopoulos Alexandros , Liakakos Theodore , Alexandrou Andreas
Published: February 2024

Background

The incidence of single-nucleotide-polymorphisms with malignant potential in esophageal cancer tissues has only been sparsely investigated in the west. Hence, we explored the contribution of four long non-coding RNAs’ polymorphisms HOTAIR rs920778, LINC00951 rs11752942, POLR2E rs3787016 and HULC rs7763881 in esophageal cancer susceptibility.

Methods and results

Formalin-fixed paraffin-embedded tissue specimens from 95 consecutive patients operated for esophageal/esophagogastric junction carcinoma during 25/03/2014-25/09/2018 were processed. Demographic data, histopathological parameters, surgical and oncological outcomes were collected. DNA findings of the abovementioned population were compared with 121 healthy community controls. Both populations were of European/Greek ancestry. Sixty-seven patients underwent Ivor Lewis/McKeown esophagectomy for either squamous cell esophageal carcinoma (N = 6) or esophageal/esophagogastric junction Siewert I or II adenocarcinoma (N = 61). Twenty-eight patients were subjected to extended total gastrectomy for esophagogastric junction Siewert III adenocarcinoma. Neither LINC00951 rs11752942 nor HULC rs7763881 polymorphisms were detected more frequently in esophageal cancer patients compared with healthy community subjects. A significantly higher presence of HOTAIR rs920778 TT genotype in esophagogastric junction Siewert I/II adenocarcinoma was identified. POLR2E rs3787016 C allele and CC genotypes were overrepresented in the control group, and when found in esophageal cancer carriers were associated with earlier disease stages, as well as with minor lymph node involvement and lesser metastatic potential.

Conclusions

HOTAIR rs920778 may serve as a potential therapeutic suppression target, while POLR2E rs3787016 may represent a valuable biomarker to evaluate esophageal cancer predisposition and predict treatment response and prognosis. Clinical implications of these findings need to be verified with further prospective studies with larger sample-size.
Evaluating contouring accuracy and dosimetry impact of current MRI-guided adaptive radiation therapy for brain metastases: a retrospective study
Authors: Wang Bin , Liu Yimei , Zhang Jun , Yin Shaohan , Liu Biaoshui , Ding Shouliang , Qiu Bo , Deng Xiaowu
Published: February 2024

Background

Magnetic resonance imaging (MRI) guided adaptive radiotherapy (MRgART) has gained increasing attention, showing clinical advantages over conventional radiotherapy. However, there are concerns regarding online target delineation and modification accuracy. In our study, we aimed to investigate the accuracy of brain metastases (BMs) contouring and its impact on dosimetry in 1.5 T MRI-guided online adaptive fractionated stereotactic radiotherapy (FSRT).

Methods

Eighteen patients with 64 BMs were retrospectively evaluated. Pre-treatment 3.0 T MRI scans (gadolinium contrast-enhanced T1w, T1c) and initial 1.5 T MR-Linac scans (non-enhanced online-T1, T2, and FLAIR) were used for gross target volume (GTV) contouring. Five radiation oncologists independently contoured GTVs on pre-treatment T1c and initial online-T1, T2, and FLAIR images. We assessed intra-observer and inter-observer variations and analysed the dosimetry impact through treatment planning based on GTVs generated by online MRI, simulating the current online adaptive radiotherapy practice.

Results

The average Dice Similarity Coefficient (DSC) for inter-observer comparison were 0.79, 0.54, 0.59, and 0.64 for pre-treatment T1c, online-T1, T2, and FLAIR, respectively. Inter-observer variations were significantly smaller for the 3.0 T pre-treatment T1c than for the contrast-free online 1.5 T MR scans (P < 0.001). Compared to the T1c contours, the average DSC index of intra-observer contouring was 0.52‒0.55 for online MRIs. For BMs larger than 3 cm3, visible on all image sets, the average DSC indices were 0.69, 0.71 and 0.64 for online-T1, T2, and FLAIR, respectively, compared to the pre-treatment T1c contour. For BMs < 3 cm3, the average visibility rates were 22.3%, 41.3%, and 51.8% for online-T1, T2, and FLAIR, respectively. Simulated adaptive planning showed an average prescription dose coverage of 63.4‒66.9% when evaluated by ground truth planning target volumes (PTVs) generated on pre-treatment T1c, reducing it from over 99% coverage by PTVs generated on online MRIs.

Conclusions

The accuracy of online target contouring was unsatisfactory for the current MRI-guided online adaptive FSRT. Small lesions had poor visibility on 1.5 T non-contrast-enhanced MR-Linac images. Contour inaccuracies caused a one-third drop in prescription dose coverage for the target volume. Future studies should explore the feasibility of contrast agent administration during daily treatment in MRI-guided online adaptive FSRT procedures.
Mangrove species found in contrasting environments show differing phytohormonal responses to variation in soil bulk density
Authors: Ola Anne , Dodd Ian C. , Albacete Alfonso , Xiong Yanmei , Rasmussen Amanda , De Diego Nuria , Lovelock Catherine E.
Published: February 2024

Background and aims

Mangrove species respond to variation in soil bulk density (BD). However, very little is known about the regulatory mechanisms that trigger these responses.

Methods

Endogenous concentrations of different phytohormones were measured in the roots of two mangrove species (Avicennia marina and Rhizophora stylosa) grown in low and high BD soils. The potential involvement of ethylene in regulating plant growth responses was tested by applying the ethylene biosynthesis inhibitors cobalt chloride (CoCl2) and aminoisobutyric acid (AIB).

Results

The two mangrove species responded differently to variation in soil BD. High BD decreased root growth of R. stylosa, but not A. marina. Soil BD had no effect on root phytohormone levels in R. stylosa, but loose soils increased 1-aminocyclopropane-1-carboxylic acid whilst decreasing salicylic acid and gibberellin in A. marina. Applying ethylene inhibitors enhanced R. stylosa root growth, while increasing indole-3-acetic acid but decreasing isopentenyl adenine levels. In contrast, AIB inhibited A. marina root growth, while increasing trans-zeatin levels. Ethylene inhibitors affected salicylic acid levels in both species.

Conclusion

Salicylic acid is central to root growth responses to variation in BD in A. marina. Conversely, the interaction of ethylene and gibberellin drives responses in R. stylosa. Hormonal interactions involving ethylene potentially reflect the adaptations of the two species to differing conditions within the intertidal zone, with A. marina behaving like an aquatic species and R. stylosa behaving like a terrestrial species.
Investigation of artificial intelligence in SMEs: a systematic review of the state of the art and the main implementation challenges
Authors: Oldemeyer Leon , Jede Andreas , Teuteberg Frank
Published: February 2024

While the topic of artificial intelligence (AI) in multinational enterprises has been receiving attention for some time, small and medium enterprises (SMEs) have recently begun to recognize the potential of this new technology. However, the focus of previous research and AI applications has therefore mostly been on large enterprises. This poses a particular issue, as the vastly different starting conditions of various company sizes, such as data availability, play a central role in the context of AI. For this reason, our systematic literature review, based on the PRISMA protocol, consolidates the state of the art of AI with an explicit focus on SMEs and highlights the perceived challenges regarding implementation in this company size. This allowed us to identify various business activities that have been scarcely considered. Simultaneously, it led to the discovery of a total of 27 different challenges perceived by SMEs in the adoption of AI. This enables SMEs to apply the identified challenges to their own AI projects in advance, preventing the oversight of any potential obstacles or risks. The lack of knowledge, costs, and inadequate infrastructure are perceived as the most common barriers to implementation, addressing social, economic, and technological aspects in particular. This illustrates the need for a wide range of support for SMEs regarding an AI introduction, which covers various subject areas, like funding and advice, and differentiates between company sizes.
Diuretic Treatment in Patients with Heart Failure: Current Evidence and Future Directions—Part II: Combination Therapy
Authors: J.J Cuthbert , J.G.F Cleland , A.L Clark
Published: February 2024

Purpose of Review

Fluid retention or congestion is a major cause of symptoms, poor quality of life, and adverse outcome in patients with heart failure (HF). Despite advances in disease-modifying therapy, the mainstay of treatment for congestion—loop diuretics—has remained largely unchanged for 50 years. In these two articles (part I: loop diuretics and part II: combination therapy), we will review the history of diuretic treatment and current trial evidence for different diuretic strategies and explore potential future directions of research.

Recent Findings

We will assess recent trials, including DOSE, TRANSFORM, ADVOR, CLOROTIC, OSPREY-AHF, and PUSH-AHF, and assess how these may influence current practice and future research.

There are few data on which to base diuretic therapy in clinical practice. The most robust evidence is for high-dose loop diuretic treatment over low-dose treatment for patients admitted to hospital with HF, yet this is not reflected in guidelines. There is an urgent need for more and better research on different diuretic strategies in patients with HF.
Robust detection of clinically relevant features in single-cell RNA profiles of patient-matched fresh and formalin-fixed paraffin-embedded (FFPE) lung cancer tissue
Authors: Trinks Alexandra , Milek Miha , Beule Dieter , Kluge Julie , Florian Stefan , Sers Christine , Horst David , Morkel Markus , Bischoff Philip
Published: February 2024

Purpose

Single-cell transcriptional profiling reveals cell heterogeneity and clinically relevant traits in intra-operatively collected patient-derived tissue. So far, single-cell studies have been constrained by the requirement for prospectively collected fresh or cryopreserved tissue. This limitation might be overcome by recent technical developments enabling single-cell analysis of FFPE tissue.

Methods

We benchmark single-cell profiles from patient-matched fresh, cryopreserved and archival FFPE cancer tissue.

Results

We find that fresh tissue and FFPE routine blocks can be employed for the robust detection of clinically relevant traits on the single-cell level. Specifically, single-cell maps of fresh patient tissues and corresponding FFPE tissue blocks could be integrated into common low-dimensional representations, and cell subtype clusters showed highly correlated transcriptional strengths of signaling pathway, hallmark, and clinically useful signatures, although expression of single genes varied due to technological differences. FFPE tissue blocks revealed higher cell diversity compared to fresh tissue. In contrast, single-cell profiling of cryopreserved tissue was prone to artifacts in the clinical setting.

Conclusion

Our analysis highlights the potential of single-cell profiling in the analysis of retrospectively and prospectively collected archival pathology cohorts and increases the applicability in translational research.
Natural and engineered xylosyl products from microbial source
Authors: Qi Jianzhao , Kang Shi-jie , Zhao Ling , Gao Jin‑ming , Liu Chengwei
Published: February 2024

Glycosylation is a prevalent post-modification found in natural products and has a significant impact on the structural diversity and activity variation of natural products. Glucosylation is the most common type of glycosylation, whereas xylosylation is relatively rare. Despite their unique chemical structures and beneficial activities, xylosylated natural products from microorganisms have received little attention. This review provides, for the first time, a comprehensive summary of 126 microbial-derived xylosylated natural products, including xylosyl-cyathane diterpenes, xylosylated triterpenes, xylosyl aromatic compounds, and others. Among these compounds, xylosyl-cyathane diterpenes represent the highest number of derivatives, followed by xylosylated triterpenes. Xylosyl compounds from bacterial sources have less defined structural profiles compared to those from fungi. The characterization of xylosyltransferase EriJ from Basidiomycota extended the structural diversity of xylosyl cyathane diterpenes. This work provides a valuable reference for the research and use of xylosyltransferase for drug discovery and synthetic chemistry. Further work is needed to explore the potential applications of microbial derived xylosyl compounds and to develop novel xylosyl transferases. With the deepening of genomic sequencing of medicinal fungi, more biosynthesis of bioactive xylosyl compounds is expected to be elucidated in the future.

Graphical Abstract
Ability to Simulate Absorption and Melt Pool Dynamics for Laser Melting of Bare Aluminum Plate: Results and Insights from the 2022 Asynchronous AM-Bench Challenge
Authors: Simonds Brian J. , Tanner Jack , Artusio-Glimpse Alexandra , Parab Niranjan , Zhao Cang , Sun Tao , Williams Paul A.
Published: February 2024

The 2022 Asynchronous AM-Bench challenge was designed to test the ability of simulations to accurately predict laser power absorption as well as various melt pool behaviors (width, depth, and solidification) during laser melting of solid metal during stationary and scanned laser illumination. In this challenge, participants were asked to predict a series of experimental outcomes. Experimental data were obtained from a series of experiments performed at the Advanced Photon Source at Argonne National Laboratories in 2019. These experiments combined integrating sphere radiometry with high-speed X-ray imaging, allowing for the simultaneous recording of absolute laser power absorption and two-dimensional, projected images of the melt pool. All challenge problems were based on experiments using bare aluminum solid metal. Participants were provided with pertinent experimental information like laser power, scan speed, laser spot size, and material composition. Additionally, participants were given absorptance and X-ray imaging data from stationary and scanned laser experiments on solid Ti–6Al–4V that could be used for testing their models before attempting challenge problems. In total, this challenge received 56 submissions from eight different research groups for eight individual challenge problems. The data for this challenge, and associated information, are available for download from the NIST Public Data Repository. This paper summarizes the results from the 2022 Asynchronous AM-Bench challenge as well as discusses the lessons learned to help inform future challenges.
The Marrakesh Treaty to Facilitate Access to Published Works for Persons Who Are Blind, Visually Impaired or Otherwise Print Disabled in the European Union: Reflecting on Its Implementation and Gauging Its Impact from a Disability Perspective
Authors: Ferri Delia
Published: February 2024

The Marrakesh Treaty to Facilitate Access to Published Works for Persons Who Are Blind, Visually Impaired or Otherwise Print Disabled (Marrakesh Treaty) requires State Parties to introduce limitations and exceptions to national copyright rules in order to allow the reproduction, distribution and making available of published works in formats that are accessible to persons who are blind, visually impaired or otherwise print disabled. This Treaty was concluded by the European Union (EU) on behalf of its Member States in 2018. It was then implemented by means of a Directive and a Regulation. On the tenth anniversary of its adoption, and six years after the enactment of the EU implementing acts, this article reflects on the implementation of the Marrakesh Treaty in the EU, highlighting its strengths and shortcomings. It then discusses, from a disability perspective, the impact of the Marrakesh Treaty within Europe on foot of a multimethod empirical study conducted within the projectReCreating Europe. On the whole, this article argues that, in spite of some weaknesses of the Marrakesh Directive, which leaves room for differences across the Member States, the ratification of the Marrakesh Treaty by the EU has supported access to printed materials for people with disabilities.
Territoriality and the Quest for a Unitary Copyright Title
Authors: van Eechoud Mireille M. M.
Published: February 2024

After 30 years of harmonization at EU level, copyright and related rights remain decidedly territorial in scope. This is despite the continuous quest for an internal market and the profound impact on cross-border creation, dissemination and use of cultural content. This contribution recounts the outcome of research done on territoriality in the context of the ReCreating Europe project. It discusses why national territorial rights persist, what type of legal mechanisms the EU legislature employs to address the adverse effects of territoriality, and sketches a number of models for a unitary title based on Art. 118 TFEU which could be explored going forward.
Lessons From the COVID-19 Unplanned Transition to Online Learning at Home for Students With Disability: Socialisation, Technology, Education and Future Research Opportunities
Authors: Young Kirsty , Clerke Teena
Published: February 2024

Purpose of Review

Periods of transition for children and adolescents with disability are complex but usually anticipated. The COVID-19 pandemic resulted in an unexpected transition to online learning at home for students. This review examines the literature on the transition to online learning at home for students with disabilities in K–12 education during the COVID-19 pandemic and identifies the challenges and perceived benefits that emerged for these students to inform future planned or unplanned transitions to online learning.

Recent Findings

Thirty-one articles met the inclusion criteria. Overall, the transition to online learning generated significant social and technical challenges for students with disability—ranging from inadequate socialisation and engagement issues to technological barriers. There were also notable benefits. For some students, this included fewer social pressures, improvements in mental health and the development of personal skills such as resilience. Parent and caregiver participation in the online learning tasks was crucial, often leading to deeper insights into the learning preferences and needs of students with disability and the ability to tailor and customise learning activities. The review identifies a need for inclusive research methods that actively involve students with disability in research on their online learning experiences. Furthermore, the predominant focus on boys with disability and the lack of representation of specific types of disability and support needs suggest that in future larger studies should strive for a more diverse and representative sample of the students with disability.

Challenges and benefits reveal much about the disruptive impact the COVID-19 online learning transition exacted on some school-aged students with disability and their families. Greater attention to increasing access to technologies required for online learning is needed. The findings of this review can be applied in the adaptation of existing, or implementation of new learning modalities, accommodations and support for students with disability to promote their inclusion and access to education. Future research should explore a more inclusive approach and identify longer-term impacts of online learning on COVID-19 cohorts of students with disability and identify ways that schools can plan for more effective transition to online learning in the event of a future pandemic or other disaster requiring school closures.