bluuebunny/crossref_metadata_2025 huggingface dataset:
Changes in DNA Methylation in Response to 6-Benzylaminopurine Affect Allele-Specific Gene Expression in Populus Tomentosa
Authors: Xuan Anran , Song Yuepeng , Bu Chenhao , Chen Panfei , El-Kassaby Yousry A. , Zhang Deqiang
Published: March 2020

Cytokinins play important roles in the growth and development of plants. Physiological and photosynthetic characteristics are common indicators to measure the growth and development in plants. However, few reports have described the molecular mechanisms of physiological and photosynthetic changes in response to cytokinin, particularly in woody plants. DNA methylation is an essential epigenetic modification that dynamically regulates gene expression in response to the external environment. In this study, we examined genome-wide DNA methylation variation and transcriptional variation in poplar (Populus tomentosa) after short-term treatment with the synthetic cytokinin 6-benzylaminopurine (6-BA). We identified 460 significantly differentially methylated regions (DMRs) in response to 6-BA treatment. Transcriptome analysis showed that 339 protein-coding genes, 262 long non-coding RNAs (lncRNAs), and 15,793 24-nt small interfering RNAs (siRNAs) were differentially expressed under 6-BA treatment. Among these, 79% were differentially expressed between alleles in P. tomentosa, and 102,819 allele-specific expression (ASE) loci in 19,200 genes were detected showing differences in ASE levels after 6-BA treatment. Combined DNA methylation and gene expression analysis demonstrated that DNA methylation plays an important role in regulating allele-specific gene expression. To further investigate the relationship between these 6-BA-responsive genes and phenotypic variation, we performed SNP analysis of 460 6-BA-responsive DMRs via re-sequencing using a natural population of P. tomentosa, and we identified 206 SNPs that were significantly associated with growth and wood properties. Association analysis indicated that 53% of loci with allele-specific expression had primarily dominant effects on poplar traits. Our comprehensive analyses of P. tomentosa DNA methylation and the regulation of allele-specific gene expression suggest that DNA methylation is an important regulator of imbalanced expression between allelic loci.
Analysis of the Presence of Most Best-Ranked Universities on Social Networking Sites
Authors: Valerio-Ureña Gabriel , Herrera-Murillo Dagoberto , Madero-Gómez Sergio
Published: March 2020

Universities are becoming aware of the importance of social networking sites for the reinforcement of their institutional brands. This study was conducted to analyze the extent to which best-ranked universities use social networking sites. A quantitative methodology and digital methods were used to measure the social media presence of the first 400 universities listed in the QS (Quacquarelli Symonds) World University Ranking 2018. The results reveal a high degree of involvement of those universities on social media; some factors that are associated with significant differences in the adoption of social networking sites include the public versus private management model, the level of academic prestige, and the region of origin.
Review of Eyeless Pseudosinella Schäffer (Collembola, Entomobryidae, and Lepidocyrtinae) from Brazilian Caves
Authors: Cipola Nikolas G. , Oliveira João Victor L. C. , Bellini Bruno C. , Ferreira Aila S. , Lima Estevam C. A. , Brito Roniere A. , Stievano Luis C. , Souza Paolla G. C. , Zeppelini Douglas
Published: March 2020

Herein, eyeless Pseudosinella species from Brazilian caves are reviewed, including the description of 23 new species, new records plus additional notes on the descriptions of P. ambigua Zeppelini, Brito, and Lima and of P. guanhaensis Zeppelini, Brito, and Lima. We also provide an identification key to 27 eyeless species recorded from Brazil. To organize the 26 Brazilian eyeless taxa analyzed in this work, we organize them in apparently artificial groups: 11 species have one larger tooth on the unguiculus outer lamella (petterseni group); one presents unguiculus outer lamella smooth or serrated (never with a larger tooth), with 9 held prelabral chaetae undivided and the last 6 held prelabral chaetae bifurcated. The Brazilian species of eyeless Pseudosinella herein described present a remarkably conservate dorsal chaetotaxy; therefore, the main diagnostic characters are related to other features like prelabral, labral, and ventral head chaetotaxy and empodial complex morphology. In addition, our study suggests that Brazilian caves possibly shelter a great diversity of Pseudosinella taxa, several of them potentially cave dependent.
Patients with Axial Spondyloarthritis Are at Risk of Developing Adhesive Capsulitis: Real-World Evidence Database Study in Taiwan
Authors: Huang Shih-Wei , Wang Jr-Yi , Lin Che-Li , Huang Chi-Chang , Liou Tsan-Hon , Lin Hui-Wen
Published: March 2020

Patients with axial spondyloarthritis (ax-SpA) present with inflammation invading the axial skeleton. Symptoms of ax-SpA interfere with patients’ quality of life, and peripheral symptoms are also noted. Human leukocyte antigen B27 was associated with adhesive capsulitis. However, epidemiological studies investigating the associated incidence and risk factors for patients with ax-SpA with adhesive capsulitis are limited. The data of patients with ax-SpA were recorded during the 2004–2008 period and followed to the end of 2010. The control cohort comprised age- and sex-matched non-ax-SpA subjects. A Cox multivariate proportional hazards model was applied to analyze the risk factors for adhesive capsulitis. The hazard ratio (HR) and adjusted hazard ratio (aHR) were estimated between the study and control cohorts after confounders were adjusted for. Effects of sulfasalazine (SSZ), methotrexate (MTX), and hydroxychloroquine (HCQ) use on adhesive capsulitis risk were also analyzed. We enrolled 2859 patients with ax-SpA in the study cohort and 11,436 control subjects. A higher incidence of adhesive capsulitis was revealed in the ax-SpA cohort: The crude HR was 1.63 (95% CI, 1.24–2.13; p < 0.001), and the aHR was 1.54 (95% CI, 1.16–2.05; p = 0.002). For patients with ax-SpA using SSZ or HCQ, no difference in aHR was noted compared with control participants, but patients with ax-SpA treated with MTX had higher HR and aHR than controls. Patients with ax-SpA are at risk for adhesive capsulitis. When these patients receive SSZ or HCQ, the risk of adhesive capsulitis can be lowered compared with that of the control cohort.
Prediction of the Epidemic Peak of Coronavirus Disease in Japan, 2020
Authors: Kuniya Toshikazu
Published: March 2020

The first case of coronavirus disease 2019 (COVID-19) in Japan was reported on 15 January 2020 and the number of reported cases has increased day by day. The purpose of this study is to give a prediction of the epidemic peak for COVID-19 in Japan by using the real-time data from 15 January to 29 February 2020. Taking into account the uncertainty due to the incomplete identification of infective population, we apply the well-known SEIR compartmental model for the prediction. By using a least-square-based method with Poisson noise, we estimate that the basic reproduction number for the epidemic in Japan is R 0 = 2.6 ( 95 % CI, 2.4 – 2.8 ) and the epidemic peak could possibly reach the early-middle summer. In addition, we obtain the following epidemiological insights: (1) the essential epidemic size is less likely to be affected by the rate of identification of the actual infective population; (2) the intervention has a positive effect on the delay of the epidemic peak; (3) intervention over a relatively long period is needed to effectively reduce the final epidemic size.
Hepatitis C Virus: Evading the Intracellular Innate Immunity
Authors: Ferreira Ana Rita , Ramos Bruno , Nunes Alexandre , Ribeiro Daniela
Published: March 2020

Hepatitis C virus (HCV) infections constitute a major public health problem and are the main cause of chronic hepatitis and liver disease worldwide. The existing drugs, while effective, are expensive and associated with undesirable secondary effects. There is, hence, an urgent need to develop novel therapeutics, as well as an effective vaccine to prevent HCV infection. Understanding the interplay between HCV and the host cells will certainly contribute to better comprehend disease progression and may unravel possible new cellular targets for the development of novel antiviral therapeutics. Here, we review and discuss the interplay between HCV and the host cell innate immunity. We focus on the different cellular pathways that respond to, and counteract, HCV infection and highlight the evasion strategies developed by the virus to escape this intracellular response.
Associations between Depressive Symptoms and Satisfaction with Meaningful Activities in Community-Dwelling Japanese Older Adults
Authors: Maruta Michio , Makizako Hyuma , Ikeda Yuriko , Miyata Hironori , Nakamura Atsushi , Han Gwanghee , Shimokihara Suguru , Tokuda Keiichiro , Kubozono Takuro , Ohishi Mitsuru , Tomori Kounosuke , Tabira Takayuki
Published: March 2020

The aim of this cross-sectional study was to investigate relationships between individuals’ ratings of satisfaction and performance of activities that they found meaningful and depressive symptoms. Data was obtained from 806 older adults (mean age 74.9 ± 6.3 years, women = 63.0%) who participated in a community-based health check survey (Tarumizu Study 2018). Participants selected meaningful activities from 95 activities using the Aid for Decision-Making in Occupation Choice and evaluated their satisfaction and performance. Depressive symptoms were assessed using the 15-item Geriatric Depression Scale (GDS-15) and defined by a GDS-15 score of ≥5. Non-linear logistic regression analyses were used separately by gender to examine the association between satisfaction and performance of meaningful activities and depressive symptoms. The prevalence of depressive symptoms was 15.8%. We found no significant difference between meaningful activity choice between older adults with depressive symptoms and those without, in both men and women. After adjusting for potential covariates, satisfaction was associated with depressive symptoms in both men (OR 0.52, 95% CI 0.35–0.77) and women (OR 0.67, 95% CI 0.49–0.91), but performance was limited in women (OR 0.87, 95% CI 0.77–0.99). Our findings suggest that depressive symptoms are associated with satisfaction in meaningful activities regardless of activity categories.
Comparison of Renal Effects of Ezetimibe–Statin Combination versus Statin Monotherapy: A Propensity-Score-Matched Analysis
Authors: Bae Jaehyun , Hong Namki , Lee Byung-Wan , Kang Eun Seok , Cha Bong-Soo , Lee Yong-ho
Published: March 2020

Neither lowering of blood lipid levels nor treatment with statins definitively improves renal outcomes. Ezetimibe, a non-statin antilipidemic agent, is known to not only decrease blood lipid levels but also reduce inflammatory response and activate autophagy. We evaluated the effect of adding ezetimibe to a statin on renal outcome compared with statin monotherapy by analyzing longitudinal data of 4537 patients treated with simvastatin 20 mg plus ezetimibe 10 mg (S + E) or simvastatin 20 mg alone (S) for more than 180 days. A propensity-score-based process was used to match baseline characteristics, medical history, and estimated glomerular filtration rate (eGFR) between S + E and S groups. Changes in serum creatinine and incidence of renal events, defined as doubling of serum creatinine to ≥1.5 mg/dL or occurrence of end-stage renal disease after the first day of treatment initiation, were compared between the groups. Among 3104 well-matched patients with a median follow-up of 4.2 years, the S + E group showed a significantly lower risk of renal events than the S group (hazard ratio 0.58; 95% CI 0.35-0.95, P = 0.032). In addition, the S + E group tended to preserve renal function compared with the S group throughout follow-up, as assessed by serum creatinine changes (P-values for time–group interactions <0.001). These data support the beneficial effects on renal function when combining ezetimibe with a statin.
Risk Factors for Difficult Peripheral Intravenous Cannulation. The PIVV2 Multicentre Case-Control Study
Authors: Rodriguez-Calero Miguel Angel , de Pedro-Gomez Joan Ernest , Molero-Ballester Luis Javier , Fernandez-Fernandez Ismael , Matamalas-Massanet Catalina , Moreno-Mejias Luis , Blanco-Mavillard Ian , Moya-Suarez Ana Belén , Personat-Labrador Celia , Morales-Asencio José Miguel
Published: March 2020

Background. Difficult peripheral intravenous cannulation (DPIVC) is associated with serious complications related to vascular access. These complications might be avoided if the risk factors were identified previously, enabling the detection of potentially difficult situations at an early stage. The aim of this study is to consider these risk factors, to determine the influence of the hospital setting, to examine the association between DPIVC and the different techniques of catheter insertion and to analyse the importance of the clinician’s experience in this context. Methods. Case-control study following a previously published protocol, conducted in 48 units of eight public hospitals in Spain. Adult patients requiring a peripheral intravenous cannula were prospectively included in the study population during their hospital stay. Over a period of 11 months, for consecutive eligible patients, nurses in each participating unit recorded data on their assessment of the vascular access performed and the technique used. Variables related to these medical personnel were also recorded. One of the researchers reviewed the patients’ clinical history to compile the relevant health variables and to characterise the healthcare process. The statistical analysis included association tests among the main study variables. The risk factors were analysed using bivariate logistic regression. The variables found to be statistically significant were included in a multivariate logistic regression model incorporating each of the healthcare environments identified. Results. The study population was composed of 2662 patients, of whom 221 (8.3%) presented with DPIVC. A previous history of difficulty, the presence of non-palpable veins, acute upper limb alterations and punctures in the ante-cubital fossa were found to be independent risk factors for DPIVC. Differences were found in the frequency of occurrence of DPIVC and in some risk factors, according to the healthcare context. The variables related to the characteristics of the hospital personnel did not influence the study event. Conclusion: The present study identifies four independent risk factors for DPIVC that can be incorporated into algorithms aimed at preventing its occurrence and facilitating the referral of patients to vascular access specialist teams.
On-Treatment Decrease of Serum Interleukin-6 as a Predictor of Clinical Response to Biologic Therapy in Patients with Inflammatory Bowel Diseases
Authors: Caviglia Gian , Rosso Chiara , Stalla Francesco , Rizzo Martina , Massano Alessandro , Abate Maria , Olivero Antonella , Armandi Angelo , Vanni Ester , Younes Ramy , Fagoonee Sharmila , Pellicano Rinaldo , Astegiano Marco , Saracco Giorgio , Bugianesi Elisabetta , Ribaldone Davide
Published: March 2020

In patients with inflammatory bowel diseases (IBD) undergoing biologic therapy, biomarkers of treatment response are still scarce. This study aimed to evaluate whether serum zonulin, a biomarker of intestinal permeability; soluble CD163 (sCD163), a macrophage activation marker; and a panel of serum cytokines could predict the response to biologic treatment in patients with IBD. For this purpose, we prospectively enrolled 101 patients with IBD and 19 patients with irritable bowel syndrome (IBS) as a control group; 60 out of 101 patients underwent treatment with biologics. Zonulin, sCD163, and cytokines were measured at the baseline in all patients and after 10 weeks of treatment in the 60 patients who underwent biologic therapy. We observed that zonulin levels were higher in IBD patients with active disease compared to those in remission (p = 0.035), and that sCD163 values were higher in patients with IBD compared to those with IBS (p = 0.042), but no association with therapy response was observed for either biomarker. Conversely, interleukin (IL)-6, IL-8, IL-10, and tumor necrosis factor-alpha showed a significant reduction from baseline to week 10 of treatment, particularly in responder patients. By multivariate logistic regression analysis corrected for disease (Crohn’s disease or ulcerative colitis), type of biologic drug (Infliximab, Adalimumab, Vedolizumab, or Ustekinumab) and disease activity, the reduction in IL-6 values was associated with a clinical response at 12 months of biological therapy (odds ratio (OR) = 4.75, 95% confidence interval (CI) 1.25–18.02, p = 0.022). In conclusion, the measurement of serum IL-6 in biologics-treated IBD patients may allow for the prediction of response to treatment at 12 months of therapy and thus may help with tailoring personalized treatment strategies.
Effect of Low Intensity Pulsed Ultrasound (LIPUS) on Tooth Movement and Root Resorption: A Prospective Multi-Center Randomized Controlled Trial
Authors: El-Bialy Tarek , Farouk Khaled , Carlyle Terry D. , Wiltshire William , Drummond Robert , Dumore Tim , Knowlton Kevin , Tompson Bryan
Published: March 2020

The aim of this study was to evaluate the possible effect of low intensity pulsed ultrasound (LIPUS) on tooth movement and root resorption in orthodontic patients. Twenty-one patients were included in a split-mouth study design (group 1). Ten additional patients were included with no LIPUS device being used and this group was used as the negative control group (group 2). Group 1 patients were given LIPUS devices that were randomly assigned to right or left side on upper or lower arches. LIPUS was applied to the assigned side that was obtained by randomization, using transducers that produce ultrasound with a pulse frequency of 1.5 MHz, a pulse repetition rate of 1 kHz, and average output intensity of 30 mW/cm2. Cone-beam computed tomography (CBCT) images were taken before and after treatment. The extraction space dimensions were measured every four weeks and root lengths of canines were measured before and after treatment. The data were analyzed using paired t-test. The study outcome showed that the mean rate of tooth movement in LIPUS side was 0.266 ± 0.092 mm/week and on the control side was 0.232 ± 0.085 mm/week and the difference was statistically significant. LIPUS increased the rate of tooth movement by an average of 29%. For orthodontic root resorption, the LIPUS side (0.0092 ± 0.022 mm/week) showed a statistically significant decrease as compared to control side (0.0223 ± 0.022 mm/week). The LIPUS application accelerated tooth movement and minimized orthodontically induced tooth root resorption at the same time.
Does Treating Hearing Loss in Older Adults Improve Cognitive Outcomes? A Review
Authors: Amieva Hélène , Ouvrard Camille
Published: March 2020

Hearing loss is the third most prevalent health condition in older age. In recent years, research has consistently reported an association between hearing loss and mental health outcomes, including poorer cognitive performances. Whether treating hearing loss in elders improves cognition has been directly or indirectly addressed by several studies. This review aims at providing a synthesis of those results. Regarding the literature on hearing aids’ use and cognition, although the lack of interventional studies has to be underlined, observational data suggest that hearing aids positively impact long-term cognition, even though more research is necessary to ascertain this statement and provide information on the length or frequency of use required in order to observe benefits. Regarding cochlear implants in elders experiencing more severe auditory deprivation, the literature is scarcer. The available studies have many limitations and do not allow the drawing of clear conclusions. Taken together, the results are encouraging. Nevertheless, because hearing loss is suspected to account for 9% of dementia cases, and also because hearing loss is one of the few potentially modifiable factors from a dementia prevention perspective, the need to stimulate research to have clearer knowledge of the benefits of treating hearing loss on cognitive outcomes is urgent.
Anatomical Considerations of Intramedullary Humeral Nailing and Lengthening
Authors: Schwarz Gilbert Manuel , Zak Lukas , Hirtler Lena , Wozasek Gerald Eliot
Published: March 2020

Intramedullary lengthening, in cases of extensive humeral shortening, offers the advantages of preventing external-fixator-associated problems. The humeral cavity, as the main parameter in nailing, however, has been neglected in recent literature. It was hypothesized that available implants might be too large and therefore increase the risk of intraoperative fractures. The aim of this cross-sectional study was to describe the humeral canal and how it might affect the choice of implant and the surgical approach. Thirty humeri (15 female, 15 male) from clinical patients and anatomical specimens were studied. Specifically, the medullary cavity width (MCW), cortical thickness (CoT), and the course of the medullary canal were examined. The smallest MCW diameters were found at the distal third of the humeral shaft with mean diameters of 10.15 ± 1.96 mm. CoTs of female humeri were significantly smaller than those of male humeri (p < 0.001). The mean angles of the pro- and recurvatum were 4.01 ± 1.68° and 10.03 ± 2.25°, and the mean valgus bending was 3.37 ± 1.58°. Before implanting a straight lengthening nail into a doubly curved humerus, X-rays and, in selected cases, CT-scans should be performed. The unique size and course of the humeral canal favors an antegrade approach in cases of intramedullary lengthening.
Assessing the Implementation of Pharmacogenomic Panel-Testing in Primary Care in the Netherlands Utilizing a Theoretical Framework
Authors: van der Wouden Cathelijne H. , Paasman Ellen , Teichert Martina , Crone Matty R. , Guchelaar Henk-Jan , Swen Jesse J.
Published: March 2020

Despite overcoming many implementation barriers, pharmacogenomic (PGx) panel-testing is not routine practice in the Netherlands. Therefore, we aim to study pharmacists’ perceived enablers and barriers for PGx panel-testing among pharmacists participating in a PGx implementation study. Here, pharmacists identify primary care patients, initiating one of 39 drugs with a Dutch Pharmacogenetic Working Group (DPWG) recommendation and subsequently utilizing the results of a 12 gene PGx panel test to guide dose and drug selection. Pharmacists were invited for a general survey and a semi-structured interview based on the Tailored Implementation for Chronic Diseases (TICD) framework, aiming to identify implementation enablers and barriers, if they had managed at least two patients with actionable PGx results. In total, 15 semi-structured interviews were performed before saturation point was reached. Of these, five barrier themes emerged: (1) unclear procedures, (2) undetermined reimbursement for PGx test and consult, (3) insufficient evidence of clinical utility for PGx panel-testing, (4) infrastructure inefficiencies, and (5) HCP PGx knowledge and awareness; and two enabler themes: (1) pharmacist perceived role in delivering PGx, and (2) believed clinical utility of PGx. Despite a strong belief in the beneficial effects of PGx, pharmacists’ barriers remain, an these hinder implementation in primary care.
Bedside 3D Visualization of Lymphatic Vessels with a Handheld Multispectral Optoacoustic Tomography Device
Authors: Giacalone Guido , Yamamoto Takumi , Belva Florence , Hayashi Akitatsu
Published: March 2020

Identification of lymphatics by Indocyanine Green (ICG) lymphography in patients with severe lymphedema is limited due to the overlying dermal backflow. Nor can the method detect deep and/or small vessels. Multispectral optoacoustic tomography (MSOT), a real-time three- dimensional (3D) imaging modality which allows exact spatial identification of absorbers in tissue such as blood and injected dyes can overcome these hurdles. However, MSOT with a handheld probe has not been performed yet in lymphedema patients. We conducted a pilot study in 11 patients with primary and secondary lymphedema to test whether lymphatic vessels could be detected with a handheld MSOT device. In eight patients, we could not only identify lymphatics and veins but also visualize their position and contractility. Furthermore, deep lymphatic vessels not traceable by ICG lymphography and lymphatics covered by severe dermal backflow, could be clearly identified by MSOT. In three patients, two of which had advanced stage lymphedema, only veins but no lymphatic vessels could be identified. We found that MSOT can identify and image lymphatics and veins in real-time and beyond the limits of near-infrared technology during a single bedside examination. Given its easy use and high accuracy, the handheld MSOT device is a promising tool in lymphatic surgery.
Clinical, Immunological, and Functional Characterization of Six Patients with Very High IgM Levels
Authors: Gallo Vera , Cirillo Emilia , Prencipe Rosaria , Lepore Alessio , Del Vecchio Luigi , Scalia Giulia , Martinelli Vincenzo , Di Matteo Gigliola , Saunders Carol , Durandy Anne , Moschese Viviana , Leonardi Antonio , Giardino Giuliana , Pignata Claudio
Published: March 2020

Very high IgM levels represent the hallmark of hyper IgM (HIGM) syndromes, a group of primary immunodeficiencies (PIDs) characterized by susceptibility to infections and malignancies. Other PIDs not fulfilling the diagnostic criteria for HIGM syndromes can also be characterized by high IgM levels and susceptibility to malignancies. The aim of this study is to characterize clinical phenotype, immune impairment, and pathogenic mechanism in six patients with very high IgM levels in whom classical HIGM syndromes were ruled out. The immunological analysis included extended B-cell immunophenotyping, evaluation of class switch recombination and somatic hypermutation, and next generation sequencing (NGS). Recurrent or severe infections and chronic lung changes at the diagnosis were reported in five out of six and two out of six patients, respectively. Five out of six patients showed signs of lymphoproliferation and four patients developed malignancies. Four patients showed impaired B-cell homeostasis. Class switch recombination was functional in vivo in all patients. NGS revealed, in one case, a pathogenic mutation in PIK3R1. In a second case, the ITPKB gene, implicated in B- and T-cell development, survival, and activity was identified as a potential candidate gene. Independent of the genetic basis, very high IgM levels represent a risk factor for the development of recurrent infections leading to chronic lung changes, lymphoproliferation, and high risk of malignancies.
Updating the Definition of the Alcohol Hangover
Authors: Verster Joris C. , Scholey Andrew , van de Loo Aurora J.A.E. , Benson Sarah , Stock Ann-Kathrin
Published: March 2020

In 2016, the Alcohol Hangover Research Group defined the alcohol hangover as “the combination of mental and physical symptoms experienced the day after a single episode of heavy drinking, starting when blood alcohol concentration (BAC) approaches zero”. In the light of new findings and evidence, we carefully reviewed the different components of that definition. Several studies demonstrated that alcohol hangovers are not limited to heavy drinking occasions. Instead, data from both student and non-student samples revealed that at a group level, alcohol hangover may occur at much lower BAC levels than previously thought. Regression analysis further revealed that for individual drinkers, the occurrence of hangovers is more likely when subjects consume more alcohol than they usually do. However, hangovers may also occur at a drinker’s usual BAC, and in some cases even at lower BAC (e.g. in case of illness). We also carefully reviewed and modified other parts of the definition. Finally, hangovers are not necessarily limited to the ‘next day’. They can start at any time of day or night, whenever BAC approaches zero after a single dinking occasion. This may also be on the same day as the drinking occasion (e.g. when drinking in, or until the morning and subsequently having a hangover in the afternoon or evening). To better reflect the new insights and sharpen the description of the concept, we hereby propose to update the definition of the alcohol hangover as follows: “The alcohol hangover refers to the combination of negative mental and physical symptoms which can be experienced after a single episode of alcohol consumption, starting when blood alcohol concentration (BAC) approaches zero”, and recommend to use this new definition in future hangover research.
Improved Isolation and Culture of Urine-Derived Stem Cells (USCs) and Enhanced Production of Immune Cells from the USC-Derived Induced Pluripotent Stem Cells
Authors: Kim Kyeongseok , Gil Minchan , Dayem Ahmed , Choi Sangbaek , Kang Geun-Ho , Yang Gwang-Mo , Cho Sungha , Jeong Yeojin , Kim Se , Seok Jaekwon , Kwak Hee , Kumar Saha Subbroto , Kim Aram , Cho Ssang-Goo
Published: March 2020

The availability of autologous adult stem cells is one of the essential prerequisites for human stem cell therapy. Urine-derived stem cells (USCs) are considered as desirable cell sources for cell therapy because donor-specific USCs are easily and non-invasively obtained from urine. Efficient isolation, expansion, and differentiation methods of USCs are necessary to increase their availability. Here, we developed a method for efficient isolation and expansion of USCs using Matrigel, and the rho-associated protein kinase (ROCK) inhibitor, Y-27632. The prepared USCs showed significantly enhanced migration, colony forming capacity, and differentiation into osteogenic or chondrogenic lineage. The USCs were successfully reprogramed into induced pluripotent stem cells (USC-iPSCs) and further differentiated into kidney organoid and hematopoietic progenitor cells (HPCs). Using flavonoid molecules, the isolation efficiency of USCs and the production of HPCs from the USC-iPSCs was increased. Taken together, we present an improved isolation method of USCs utilizing Matrigel, a ROCK inhibitor and flavonoids, and enhanced differentiation of USC-iPSC to HPC by flavonoids. These novel findings could significantly enhance the use of USCs and USC-iPSCs for stem cell research and further application in regenerative stem cell-based therapies.
Intraoperative Autofluorescence and Indocyanine Green Angiography for the Detection and Preservation of Parathyroid Glands
Authors: Demarchi Marco Stefano , Karenovics Wolfram , Bédat Benoît , Triponez Frédéric
Published: March 2020

Fluorescence imaging is a well-known method for both the in vivo and in vitro identification of specific cells or tissues. This imaging tool is gaining importance in the intraoperative detection and preservation of parathyroid glands during endocrine surgery owing to the intrinsic properties of parathyroid tissue. The aim of this paper is to provide an overview of the basics of the technology, its history, and the recent surgical intraoperative applications of near-infrared imaging methods. Moreover, a literature review of the utilization of fluorescence devices in thyroid surgery suggests that the use of near-infrared imaging seems to be beneficial in reducing postoperative hypoparathyroidism, which is one of the most frequent complications of thyroid surgery.
Cortical Complexity in Anorexia Nervosa: A Fractal Dimension Analysis
Authors: Collantoni Enrico , Madan Christopher R. , Meneguzzo Paolo , Chiappini Iolanna , Tenconi Elena , Manara Renzo , Favaro Angela
Published: March 2020

Fractal Dimension (FD) has shown to be a promising means to describe the morphology of cortical structures across different neurologic and psychiatric conditions, displaying a good sensitivity in capturing atrophy processes. In this study, we aimed at exploring the morphology of cortical areas by means of FD in 58 female patients with Anorexia Nervosa (AN) (38 currently underweight and 20 fully recovered) and 38 healthy controls (HC). All participants underwent high-resolution MRI. Surface extraction was completed using FreeSurfer, FD was computed using the calcFD toolbox. The whole cortex mean FD value was lower in acute AN patients compared to HC (p < 0.001). Recovered AN patients did not show differences in the global FD when compared to HC. However, some brain areas showed higher FD in patients than controls, while others showed the opposite pattern. Parietal regions showed lower FD in both AN groups. In acute AN patients, the FD correlated with age (p < 0.001), body mass index (p = 0.019) and duration of illness (p = 0.011). FD seems to represent a feasible method to explore cortical complexity in patients with AN since it demonstrated to be sensitive to the effects of both severity and duration of malnutrition.
Different Pulp Dressing Materials for the Pulpotomy of Primary Teeth: A Systematic Review of the Literature
Authors: Bossù Maurizio , Iaculli Flavia , Di Giorgio Gianni , Salucci Alessandro , Polimeni Antonella , Di Carlo Stefano
Published: March 2020

Background: Pulpotomy of primary teeth provides favorable clinical results over time; however, to date, there is still not a consensus on an ideal pulp dressing material. Therefore, the aim of the present systematic review was to compare pulpotomy agents to establish a preferred material to use. Methods: After raising a PICO question, the PRISMA guideline was adopted to carry out an electronic search through the MEDLINE database to identify comparative studies on several pulp dressing agents, published up to October 2019. Results: The search resulted in 4274 records; after exclusion, a total of 41 papers were included in the present review. Mineral trioxide aggregate (MTA), Biodentine and ferric sulphate yielded good clinical results over time and might be safely used in the pulpotomies of primary molars. Among agents, MTA seemed to be the material of choice. On the contrary, calcium hydroxide showed the worst clinical performance. Although clinically successful, formocreosol should be replaced by other materials, due to its potential cytotoxicity and carcinogenicity. Conclusion: MTA seemed to be the gold standard material in the pulpotomy of primary teeth. Promising results were also provided by calcium silicate-based cements. Further randomized clinical trials (RCTs) with adequate sample sizes and long follow-ups are encouraged to support these outcomes.
Electrical Conductivity and Electromagnetic Shielding Effectiveness of Bio-Composites
Authors: Tserpes Konstantinos , Tzatzadakis Vasileios , Bachmann Jens
Published: March 2020

In this paper, the electrical conductivity and electromagnetic shielding effectiveness of two bio-composites are studied by experimental testing and numerical models. Two monolithic composites with partly bio-based content were manufactured. The first bio-composite is made of a carbon fiber fabric prepreg and a partly bio-based (rosin) epoxy resin (CF/Rosin). The second bio-composite is a combination of prepregs of carbon fiber fabric/epoxy resin and flax fiber fabric/epoxy resin (CF-Flax/Epoxy). A single line infusion process was used prior to the curing step in the autoclave. Both variants are exemplary for the possibility of introducing bio-based materials in high performance CFRP. In-plane and out-of-plane electrical conductivity tests were conducted according to Airbus standards AITM2 0064 and AITM2 0065, respectively. Electromagnetic shielding effectiveness tests were conducted based on the standard ASTM D 4935-10. Materials were prepared at the German Aerospace Center (DLR), while characterization tests were conducted at the University of Patras. In addition to the tests, numerical models of representative volume elements were developed, using the DIGIMAT software, to predict the electrical conductivity of the two bio-composites. The preliminary numerical results show a good agreement with the experimental results.
Optimized Radial Basis Function Neural Network Based Intelligent Control Algorithm of Unmanned Surface Vehicles
Authors: Wang Renqiang , Li Donglou , Miao Keyin
Published: March 2020

To improve the tracking stability control of unmanned surface vehicles (USVs), an intelligent control algorithm was proposed on the basis of an optimized radial basis function (RBF) neural network. The design process was as follows. First, the adaptation value and mutation probability were modified to improve the traditional optimization algorithm. Then, the improved genetic algorithms (GA) were used to optimize the network parameters online to improve their approximation performance. Additionally, the RBF neural network was used to approximate the function uncertainties of the USV motion system to eliminate the chattering caused by the uninterrupted switching of the sliding surface. Finally, an intelligent control law was introduced based on the sliding mode control with the Lyapunov stability theory. The simulation tests showed that the intelligent control algorithm can effectively guarantee the control accuracy of USVs. In addition, a comparative study with the sliding mode control algorithm based on an RBF network and fuzzy neural network showed that, under the same conditions, the stabilization time of the intelligent control system was 33.33% faster, the average overshoot was reduced by 20%, the control input was smoother, and less chattering occurred compared to the previous two attempts.
Surprising Behaviour of the Wageningen B-Screw Series Polynomials
Authors: Helma Stephan
Published: March 2020

Undoubtedly, the Wageningen B-screw Series is the most widely used systematic propeller series. It is very popular to preselect propeller dimensions during the preliminary design stage before performing a more thorough optimisation, but in the smaller end of the market it is often used to merely select the final propeller. Over time, the originally measured data sets were faired and scaled to a uniform Reynolds number of 2 · 106 to increase the reliability of the series. With the advent of the computer, polynomials for the thrust and torque values were calculated based on the available data sets. The measured data are typically presented in the well-known open-water curves of thrust and torque coefficients K T and K Q versus the advance coefficient J . Changing the presentation from these diagrams to efficiency maps reveals some unsuspected and surprising behaviours, such as multiple extrema when optimising for efficiency or even no optimum at all for certain conditions, where an optimum could be expected. These artefacts get more pronounced at higher pitch to diameter ratios and low blade numbers. The present work builds upon the paper presented by the author at the AMT’17 and smp’19 conferences and now includes the extended efficiency maps, as suggested by Danckwardt, for all propellers of the Wageningen B-screw Series.
Assessment of Bankruptcy Risk of Large Companies: European Countries Evolution Analysis
Authors: Bărbuță-Mișu Nicoleta , Madaleno Mara
Published: March 2020

Assessment and estimation of bankruptcy risk is important for managers in decision making for improving a firm’s financial performance, but also important for investors that consider it prior to making investment decision in equity or bonds, creditors and company itself. The aim of this paper is to improve the knowledge of bankruptcy prediction of companies and to analyse the predictive capacity of factor analysis using as basis the discriminant analysis and the following five models for assessing bankruptcy risk: Altman, Conan and Holder, Tafler, Springate and Zmijewski. Stata software was used for studying the effect of performance over risk and bankruptcy scores were obtained by year of analysis and country. Data used for non-financial large companies from European Union were provided by Amadeus database for the period 2006–2015. In order to analyse the effects of risk score over firm performance, we have applied a dynamic panel-data estimation model, with Generalized Method of Moments (GMM) estimators to regress firm performance indicator over risk by year and we have used Tobit models to infer about the influence of company performance measures over general bankruptcy risk scores. The results show that the Principal Component Analysis (PCA) used to build a bankruptcy risk scored based on discriminant analysis indices is effective for determining the influence of corporate performance over risk.
Extensive Diversity and Disparity of the Early Miocene Platanistoids (Cetacea, Odontoceti) in the Southeastern Pacific (Chilcatay Formation, Peru)
Authors: Bianucci Giovanni , de Muizon Christian , Urbina Mario , Lambert Olivier
Published: March 2020

Several aspects of the fascinating evolutionary history of toothed and baleen whales (Cetacea) are still to be clarified due to the fragmentation and discontinuity (in space and time) of the fossil record. Here we open a window on the past, describing a part of the extraordinary cetacean fossil assemblage deposited in a restricted interval of time (19–18 Ma) in the Chilcatay Formation (Peru). All the fossils here examined belong to the Platanistoidea clade as here redefined, a toothed whale group nowadays represented only by the Asian river dolphin Platanista gangetica. Two new genera and species, the hyper-longirostrine Ensidelphis riveroi and the squalodelphinid Furcacetus flexirostrum, are described together with new material referred to the squalodelphinid Notocetus vanbenedeni and fragmentary remains showing affinities with the platanistid Araeodelphis. Our cladistic analysis defines the new clade Platanidelphidi, sister-group to Allodelphinidae and including E. riveroi and the clade Squalodelphinidae + Platanistidae. The fossils here examined further confirm the high diversity and disparity of platanistoids during the early Miocene. Finally, morphofunctional considerations on the entire platanistoid assemblage of the Chilcatay Formation suggest a high trophic partitioning of this peculiar cetacean paleocommunity.
The Process of Magnetizing FeNbYHfB Bulk Amorphous Alloys in Strong Magnetic Fields
Authors: Jeż Bartłomiej , Wysłocki Jerzy , Walters Simon , Postawa Przemysław , Nabiałek Marcin
Published: March 2020

The structure of amorphous alloys still has not been described satisfactorily due to the lack of direct methods for observing structural defects. The magnetizing process of amorphous alloys is closely related to its disordered structure. The sensitivity of the magnetization vector to any heterogeneity allows indirect assessment of the structure of amorphous ferromagnetic alloys. In strong magnetic fields, the magnetization process involves the rotation of a magnetization vector around point and line defects. Based on analysis of primary magnetization curves, it is possible to identify the type of these defects. This paper presents the results of research into the magnetization process of amorphous alloys that are based on iron, in the areas called the approach to ferromagnetic saturation and the Holstein–Primakoff para-process. The structure of a range of specially produced materials was examined using X-ray diffraction. Primary magnetization curves were measured over the range of 0 to 2 T. The process of magnetizing all of the tested alloys was associated with the presence of linear defects, satisfying the relationship Ddi p < 1H. It was found that the addition of yttrium, at the expense of hafnium, impedes the magnetization process. The alloy with an atomic content of Y = 10% was characterized by the highest saturation magnetization value and the lowest value of the Dspf parameter, which may indicate the occurrence of antiferromagnetic ordering in certain regions of this alloy sample.
Numerical Simulation on Size Effect of Fracture Toughness of Concrete Based on Mesomechanics
Authors: Wang Juan , Wu Qianqian , Guan Junfeng , Zhang Peng , Fang Hongyuan , Hu Shaowei
Published: March 2020

The fracture performance of concrete is size-dependent within a certain size range. A four-phase composite material numerical model of mesofracture considering a mortar matrix, coarse aggregates, an interfacial transition zone (ITZ) at the meso level and the initial defects of concrete was established. The initial defects were assumed to be distributed randomly in the ITZ of concrete. The numerical model of concrete mesofracture was established to simulate the fracture process of wedge splitting (WS) concrete specimens with widths of 200–2000 mm and three-point bending (3-p-b) concrete specimens with heights of 200–800 mm. The fracture process of concrete was simulated, and the peak load (Pmax) of concrete was predicted using the numerical model. Based on the simulating results, the influence of specimen size of WS and 3-p-b tests on the fracture parameters was analyzed. It was demonstrated that when the specimen size was large enough, the fracture toughness (KIC) value obtained by the linear elastic fracture mechanics formula was independent of the specimen size. Meanwhile, the improved boundary effect model (BEM) was employed to study the tensile strength (ft) and fracture toughness of concrete using the mesofracture numerical model. A discrete value of β = 1.0–1.4 was a sufficient approximation to determine the ft and KIC values of concrete.
Color Shift, Color Stability, and Post-Polishing Surface Roughness of Esthetic Resin Composites
Authors: Alkhadim Yara Khalid , Hulbah Malak Jameel , Nassar Hani Mohammad
Published: March 2020

The aim of this study was to compare color shift, color stability, and post-polishing surface roughness of esthetic restorative materials. Twenty-five disc-shaped specimens (10 mm in diameter and 2 mm in thickness) from five esthetic resin materials (Z250XT, IPS Empress-Direct, G-ænial, Vit-l-escence, and Ceram.X) were fabricated. Color shift before and immediately after light-curing (∆E0) then color stability after immersion in tea, coffee, berry juice, and distilled water were measured using a spectrophotometer. Color changes were measured after 2 (ΔE2), 4 (ΔE4), 6 (ΔE6), and 8 weeks (ΔE8). Surface roughness values (Rq) were determined using an optical profilometer after polishing using a rotary polishing system. Data were analyzed using ANOVA and multiple comparison methods at 0.05 significance level. There were no significant differences between the tested materials regarding color shift except between Vit-l-escence and Ceram.X (p = 0.033). There was no significant difference between coffee and tea solutions (p = 1.0) and between berry juice and distilled water (p = 0.15). There was no difference between the tested materials regarding Rq (p = 0.057). Ceram.X was associated with the lowest ΔE8 values overall. Tested materials were comparable regarding color shift and post-polishing surface roughness. Susceptibility to staining was dependent on the material.
Harvesting Variable-Speed Wind Energy with a Dynamic Multi-Stable Configuration
Authors: Wang Yuansheng , Zhou Zhiyong , Liu Qi , Qin Weiyang , Zhu Pei
Published: March 2020

To harvest the energy of variable-speed wind, we proposed a dynamic multi-stable configuration composed of a piezoelectric beam and a rectangular plate. At low wind speeds, the system exhibits bi-stability, whereas, at high wind speeds, the system exhibits a dynamic tri-stability, which is beneficial for harvesting variable-speed wind energy. The theoretical analysis was carried out. For validation, the prototype was fabricated, and a piezoelectric material was bonded to the beam. The corresponding experiment was conducted, with the wind speed increasing from 1.5 to 7.5 m/s. The experiment results prove that the proposed harvester could generate a large output over the speed range. The dynamic stability is helpful to maintain snap-through motion for variable-speed wind. In particular, the snap-through motion could reach coherence resonance in a range of wind speed. Thus, the system could keep large output in the environment of variable-speed wind.