bluuebunny/crossref_metadata_2025 huggingface dataset:
Sosialisasi Penggunaan Media Pembelajaran Inovatif pada Guru SMA di Karawang
Authors: Saylendra Nadya Putri , Susanto Erwin , Repelita Tridays
Published: June 2022

Pengabdian ini dilatarbelakangi oleh perlunya adaptasi perubahan zaman yang dilakukan oleh guru guna tetap dapat mengikuti perkembangan zaman dan meningkatkan kualitas pembelajaran di kelas. Adapun kegiatan ini akan dilakukan melalui zoom meeting dengan menggunakan metode sosialisasi. Sasaran dalam kegiatan ini adalah guru-guru di lingkungan SMAN 5 Karawang. Hasil dari sosialisasi ini yaitu pemahaman guru tentang kebutuhan untuk beradaptasi dalam dunia digital, teknologi dan informasi cukup meningkat. Hal tersebut dapat dilihat dari antusiasme yang mereka tunjukkan dalam interaksi yang terjadi selama kegiatan berlangsung dan pengintegrasian perangkat pembelajaran terutama pada media pembelajaran dengan teknologi adalah sebuah keharusan. Rekomendasi yaitu perlu diadakannya kegiatan serupa untuk terus me-maintenance dan meningkatkan pemahaman guru-guru agar terus dapat menyesuaikan diri dengan perkembangan zaman.
Two years of post-project monitoring of a navigation solution in a dynamic coastal environment, Smith Island, Maryland
Authors: Seiple Jacqueline , Santiago Luis , Spaur Christopher , Altman Safra , Balazik Matthew , Laczo Thomas , Mensah Daniel , Amarasingha Warunika , Payson Andrew , Szimanski Danielle
Published: June 2022

In 2018, jetties and a sill were constructed by the US Army Corps of Engineers adjacent to the Sheep Pen Gut Federal Channel at Rhodes Point, Smith Island, Maryland. These navigation improvements were constructed under Section 107 of the Continuing Authorities Program. Material dredged for construction of the structures and realignment of the channel were used to restore degraded marsh. Following construction and dredging, 2 years of monitoring were performed to evaluate the performance of navigation improvements with respect to the prevention of shoaling within the channel, shoreline changes, and impacts to submerged aquatic vegetation (SAV). Technical Report ERDC/CHL TR-20-14 describes the first year of post-project monitoring and the methodologies employed. This report describes conclusions derived from 2 years of monitoring. While the navigation improvements are largely preventing the channel from infilling, shoaling within is occurring at rates higher than expected. The placement site appears stable and accreting landward; however, there continues to be erosion along the shoreline and through the gaps in the breakwaters. SAV monitoring indicates that SAV is not present in the project footprint, even though turbidity is comparable to the reference area. Physical disturbance of the bottom sediment during construction may explain SAV absence.
Analytical forecasting of the optimal trajectory of mobile robot
Authors: Lobaty A. A. , Kapskiy D. V. , Ibrahim A. K. , Shishkovets I. A.
Published: June 2022

The purpose of the research, the results of which are presented in the article, is the analytical synthesis of the law of control of a wheeled mobile robot while it moves along a trajectory specified by reference points on the surface in an inertial coordinate system. The analysis of the existing various approaches to the formation of a given trajectory of movement of a mobile robot, based on a different mathematical formulation of the problem, is carried out. To achieve this goal, the trajectory of motion is considered to consist of separate intervals, at each of which the control optimization problem is solved. The optimization criterion in general form and its representation in the form of a minimized quadratic quality functional, convenient for analytical synthesis of control, are substantiated. As components of the functional, the parameters of the deviation of the trajectory of the mobile robot from the given points in space are considered, as well as the predicted parameters of the velocity vector and the controlling normal acceleration of the mobile robot mass center. In this case, at each given point in space, the trajectory direction to the next point is taken into account, which ensures the optimal curvature of the trajectory at a given flight speed of the aircraft. As a result of analytical synthesis, mathematical dependences were obtained to determine the control acceleration, which allow in the robot control system to obtain a given optimal control law in the form of the rotation angle of the velocity vector, which ultimately ensures the minimum energy consumption by the mobile robot for various conditions of its use. The validity of the proposed theoretical provisions is confirmed by an illustrative example, in which, for a simplified mathematical formulation of the problem, the optimal laws of changing the direction of the velocity vector and the parameters of the trajectory of a mobile robot are calculated by means of computer modeling. The illustrations show the trajectories of the robot through various given points in space and the law of changing the direction of the velocity vector.
Device models and technologies of data transmission and processing in the internet of things networks
Authors: Vishnyakov V. A.
Published: June 2022

The subject of research is the analysis and definition of the information model of devices, the choice of technologies and protocols for data collection and analysis, routing protocols for the Internet of Things (IoT) networks. The purpose of the article is to create an information model of IoT devices, to define technologies and protocols for data transmission and processing and routing in Internet of Things systems. Building an information model of an IoT device includes identifying data sources and their formats, creating a model and data structure, and analyzing them using tools such as: Eclipse Vorto ThingBoard, Ubodots IoT, Node-Red-UI, freeboard.io . An example of a description of a model of microclimate control devices using the Eclipse Vorto toolkit is given. Two main data processing technologies from IoT devices are considered: IBM Watson IoT, Cisco IWF with examples of their application. Radio frequency, infrared, optical and galvanic technologies for interaction between data collection and data transmission devices in IoT networks with examples of their use are discussed. The areas of application of three main IoT network protocols are considered: MQTT (large corporate networks), CoAP (limited networks on the Internet), Bluetooth Low Energy (local networks without the Internet with a small amount of data).Wi-Fi WebSockets, ZigBee, LoRA, Simple RF, XMPP, RFID, NFC can be used as additional protocols for networks. The features of using routing protocols in IoT networks are discussed: RPL (low power consumption), cognitive RPL (for intelligent networks), CARP (for underwater communication networks), E-CARP (for data reuse).
Construction of a system for monitoring the state of technological equipment based on computer models
Authors: Ausiyevich A. M. , Skoybeda A. T. , Dounar S. S. , Kruglikau A. A. , Robilko E. M. , Rahman D. K.
Published: June 2022

The paper describes a method for condition monitoring of a complex mechanical system based on pre-computer FEM simulation of vibrations. The interrelation of vibrations of the connected elements of the system, described by transfer functions, is shown. The occurrence of resonant vibrations in directions orthogonal to the direction of the disturbing effect is revealed. The method for calculating of orthogonal direction vibration amplitudes can be based on measuring the values of the of vibrations in one direction and the regularities of the ratio of the amplitudes of vibrations.
Regeneración ósea vertical con implante dental simultáneo en zona estética
Authors: Jiménez Castellanos Fabio Andrés , Castro Pereira Paola Andrea , Peña Pineda Wilson Orlando
Published: June 2022

Introducción: La presencia de defectos óseos dificulta el posicionamiento de los implantes, y es la deficiencia ósea vertical la que representa un mayor reto para el clínico por la alta sensibilidad de la técnica y las frecuentes complicaciones intra y posoperatorias. Una alternativa de tratamiento para los defectos verticales es la regeneración ósea guiada con implante simultáneo; sin embargo, en la literatura existen pocos estudios que evalúen su efectividad en el tiempo. Objetivo: Evaluar la estabilidad de los tejidos periimplantares en un implante dental posicionado simultáneo a la regeneración ósea vertical en una zona estética, luego de 3 años de carga funcional. Presentación de caso: Paciente masculino de 62 años que presenta ausencia del incisivo lateral derecho con un defecto óseo vertical de 3 mm, que crea una desarmonía de los puntos cenit. Posteriormente a la adecuada evaluación del caso, se decide realizar una regeneración ósea guiada vertical y colocar un implante dental simultáneo, y se obtuvo una ganancia ósea vertical de 3 mm. El implante es rehabilitado con corona en zirconio y evaluado luego de 3 años de la carga funcional. Conclusión: La regeneración ósea guiada vertical y el implante dental simultáneo en zona estética en defectos óseos pequeños (<4 mm) es una técnica quirúrgica y protésica adecuada para reducir los momentos quirúrgicos, ya que proporciona estabilidad a los tejidos periimplantares, luego de 3 años de la carga funcional del implante.
Symbiont-driven niche expansion shaped the adaptive radiation of insects
Authors: Cornwallis Charlie , Padje Anouk van't , Ellers Jacintha , Klein Malin , Jackson Raphaella , Kiers Toby , West Stuart , Henry Lee
Published: June 2022

For over 300 million years, insects have relied on symbiotic microbes for nutrition and defence1,2. However, it is unclear whether specific ecological conditions have repeatedly favoured the evolution of symbioses, and how this has influenced insect diversification1,3,4. Using data on 1849 microbe-insect symbioses across 402 insect families, we found that symbionts have allowed insects to specialize on a range of nutrient-imbalanced diets, including phloem, blood and wood. Across diets, the only limiting nutrient consistently associated with the evolution of obligate symbiosis was B vitamins. The shift to new diets, facilitated by symbionts, had mixed consequences for insect diversification. In some cases, such as herbivory, it resulted in spectacular species proliferation. In other niches, such as strict blood feeding, diversification has been severely constrained. Symbioses therefore appear to solve widespread nutrient deficiencies for insects, but the consequences for insect diversification depend on the feeding niche invaded.
Temperate Regenerative Agriculture practices increase soil carbon but not crop yield – a meta-analysis
Authors: Jordon Matthew , Willis Kathy , Bürkner Paul-Christian , Haddaway Neal , Smith Pete , Petrokofsky Gillian
Published: June 2022

Regenerative Agriculture (RA) claims to build soil organic carbon (SOC) and increase crop yields through simultaneous adoption of a suite of management practices which restore soil health. However, this claim is largely unevidenced as few studies of fully integrated regenerative systems are currently available. As a first step to addressing this knowledge gap, we here examine three practices now being promoted as part of RA: reducing tillage intensity, cover cropping and including a grass-based phase in arable rotations (ley-arable rotations). Our Bayesian meta-analysis of 195 paired SOC and crop yield observations from a systematic review of published studies finds statistically significant increases in SOC concentration for reduced tillage intensity (0.06 g C.100g-1) and ley-arable rotations (0.05 g C.100-1g per year of ley) compared to conventional practice over an average study duration of 15 years, but no effect of cover crops. None of these practices reduce yield during cropping years, although we find no evidence of a win-win between increasing SOC and enhanced agricultural productivity following adoption. While future work should evaluate the net greenhouse gas emission implications of each practice and investigate the potential for synergistic effects if RA practices are adopted in combination, our results give land managers and policy makers confidence to further adopt these practices without loss of crop yield.
A new scheme for calculating soil thermal diffusivity
Authors: Li Yujie , Gao Xiaoqing , Yang Liwei , Li Zhenchao , Zhou Ya
Published: June 2022

Land-atmosphere interaction is one of important causes of climate change. Accurate characterization of physical mechanism of the soil layer can help us better understand land-atmosphere interaction. As one of important thermal properties of soil, soil thermal diffusivity is an important parameter reflecting soil thermal conductivity and a necessary condition for simulating soil thermodynamic environment. Based on comparison of several methods for calculating soil thermal diffusivity (amplitude method, phase method, logarithmic method, arctangent method, Laplace method and numerical methods), we developed a new soil thermal diffusivity model (Thermal conduction-convection method based on Fourier series boundary condition for calculating soil thermal diffusivity, TCCMF) and compares the results of the new model with other methods. The results show that: (1) The accuracy of the results obtained by the amplitude method, phase method, logarithmic method, and arctangent method is not high enough; the Laplace method can better solve the effects of extreme weather or non-periodic changes in soil temperature. (2) When numerical methods are used to solve the thermal conduction equation, Crank-Nichalson-Sch format is unconditionally stable, the data utilization rate is higher, the obtained soil thermal diffusivity is less discrete, and the result is more accurate. (3) The new model (TCCMF) uses the Fourier series method, which is easy to calculate, and has more complete physical process and more flexible precision control. The simulation results of soil temperature using this method show that this method (TCCMF) has better simulation accuracy, indicating that it has potential value in simulating soil thermodynamic characteristics and land-atmosphere interaction.
Tectonic Geomorphology and Seismic Hazard of the East Boundry Thrust in northern segment of the Sunda-Andaman subduction zone
Authors: BHAT GULAM RASOOL , Balaji Sankaran , Ganai Maqbool Yousuf
Published: June 2022

The East Boundary Thrust (EBT), is a ~ 500 km long linear to curvilinear deformation zone along the Sunda-Andaman plate edge convergent margin. EBT is the main active tectonic structure responsible for accretionary prism formation and accommodates the bulk of present crustal deformation in the outer arc ridge of the Andaman and Nicobar subduction zone. The EBT marks the up dip expression of subduction mega thrust progressing towards the Andaman trench in the form of actively accreting the frontal prism and deforming the trench sediment sequences above the detachment. We conducted paleoseismic trench investigations of the Jarawa Thrust (JT) (northern 300km segment of EBT) at two specific sites between Ferrargunj in the south and the Radhanagar in the north Andaman. Structural and stratigraphic relationships exposed in a trench excavated across the 6 m high scarp at Ferrargunj show the scarp to be the result of single earthquake. Dating of sediment offset by the fault with optical luminescence indicates the displacement occurred between 2160 ± 300 BP and 400 ± 50 BP. Complex structural deformation is observed in the trench excavated across 10 m high scarp at Radhanagar that similarly indicates the scarp is due to single earthquake. The present findings are helpful for seismic hazard evaluation of the Andaman and Nicobar region, where greater slip deficit and no great earthquake (M ≥ 8) have been accounted so far and suggests potential for earthquakes larger than observed historically.
Comparative Investigation of Photobiomodulation in Diabetes-Impaired Alveolar Bone healing; A Histomorphometrical and Molecular Study
Authors: salari-sedigh hamideh , dalirsani zohreh , shafieian reyhaneh
Published: June 2022

Introduction:
Diabetes mellitus is increasing worldwide. Photobiomodulation is proposed as a therapeutic method in various medical concerns. This study aimed to compare the effects of photobiomodulation at the wavelength of 660, 808, or 660 + 808nm, on alveolar bone healing in diabetic rats.
Materials and methods
Bilateral maxillary first molars were extracted from diabetic Wistar rats (n = 36). Right-sided sockets were treated by an In-Ga-Al-P laser at 660nm (7.2 j/cm2, 24 s; DM660), Ga-Al-As laser at 808nm (7 j/cm2, 14 s; DM808), or a combination of these two sets (DM-dual) (n = 12). Left sides served as controls. On days 7 or 14, specimens were assigned for histomorphometric or RT-PCR analysis of RunX2, OCN, COL1 and VEGF expression.
Results
Irradiated sockets of groups DM-808 and DM-dual showed a significant increase in bone tissue and blood vessel establishment as compared to DM-660. Furthermore, group DM-dual exhibited the least amount of fibrosis tissue as compared to the other groups.
Conclusion
Within the limits of this study, our experimental model suggested photobiomodulation at 808nm, whether in form of single or combined irradiation, imposing a therapeutic influence on diabetic alveolar bone healing.
Development of an evaluation method on using electric heating in cold rural areas of China
Authors: Yu Wei , Zhou Haixia , Huang Jiaying , Yu Zixian , Wei Shen , Wu Xiaochun , Ma Xiao
Published: June 2022

Responding to the national “double carbon” policy, rural areas in China are upgrading their heating methods. Since rural areas are more dispersed than urban areas, centralized heating is not easy to use. Therefore, electric heating has become one of the major solutions. However, relevant studies on electric heating usage in rural areas are limited. In this study, therefore, research methods including literature review, questionnaire, and expert consultation have been adopted, to decide relevant indicators reflecting the suitability of using electric heating in cold rural areas in China. Then, both expert questionnaire and analytic hierarchy process were used to decide the weight of these indicators, with a hybrid model established based on the gray whitening weight clustering method. Finally, a case village was selected to validate the performance of the model, with 20 households involved. The main results from this study include: 1) the primary indicators were technology, economy, comfort, safety, aesthetic portability, and environmental protection; 2) the weights of these indicators were 0.1617, 0.3158, 0.2337, 0.1846, 0.0516, and 0.0525; 3) the evaluation result for the case village was ‘suitable’, which was consistent with the villagers' actual subjective evaluation results. The evaluation method developed in this study can effectively reflect the actual needs of people living in cold rural areas of China and can help to achieve low-carbon heating in rural areas.
Outbreak.info genomic reports: scalable and dynamic surveillance of SARS-CoV-2 variants and mutations
Authors: Hughes Laura , Gangavarapu Karthik , Latif Alaa Abdel , Mullen Julia , Alkuzweny Manar , Hufbauer Emory , Tsueng Ginger , Haag Emily , Zeller Mark , Aceves Christine , Zaiets Karina , Cano Marco , Zhou Jerry , Qian Zhongchao , Sattler Rachel , Matteson Nathaniel , Levy Joshua , Lee Raphael , Freitas Lucas , Maurer-Stroh Sebastian , Suchard Marc , Wu Chunlei , Su Andrew , Andersen Kristian
Published: June 2022

The emergence of SARS-CoV-2 variants of concern has prompted the need for near real-time genomic surveillance to inform public health interventions. In response to this need, the global scientific community, through unprecedented effort, has sequenced and shared over 11 million genomes through GISAID, as of May 2022. This extraordinarily high sampling rate provides a unique opportunity to track the evolution of the virus in near real-time. Here, we present outbreak.info, a platform that currently tracks over 40 million combinations of PANGO lineages and individual mutations, across over 7,000 locations, to provide insights for researchers, public health officials, and the general public. We describe the interpretable and opinionated visualizations in the variant and location focussed reports available in our web application, the pipelines that enable the scalable ingestion of heterogeneous sources of SARS-CoV-2 variant data, and the server infrastructure that enables widespread data dissemination via a high performance API that can be accessed using an R package. We present a case study that illustrates how outbreak.info can be used for genomic surveillance and as a hypothesis generation tool to understand the ongoing pandemic at varying geographic and temporal scales. With an emphasis on scalability, interactivity, interpretability, and reusability, outbreak.info provides a template to enable genomic surveillance at a global and localized scale.
Metadichol, a natural ligand for the expression of Yamanaka reprogramming factors in somatic and primary cancer cell lines
Authors: Raghavan P.R.
Published: June 2022

The conversion of somatic cells back into induced pluripotent stem cells (iPSCs) or embryonic-like stem cells involves the introduction of four genes commonly called Yamanaka factors: SOX2, OCT4, KLF4, and c-MYC. One drawback is that these genes and the viral vectors used to introduce them into cells have the potential to cause cancer, and these iPSC lines are not clinically useful. Most instances of direct reprogramming have been achieved by the forced expression of defined factors using viral vectors. In this paper, we show that Metadichol® has the potential to generate iPSCs non-virally and may be helpful in clinical applications. Metadichol is a Nano formulation of long-chain alcohols derived from food. Quantitative real-time PCR (qRT-PCR) and western blotting showed that OCT4, SOX2, and NANOG are expressed when fibroblasts were treated with Metadichol at concentrations of one picogram to 100 nanograms. Reverse-transcription PCR (RT-PCR) resulted in OCT4, KLF4, NANOG, and SOX2 levels increased relative to controls by 4.01, 3.51 and 1.26, and 2.5 times, respectively. Such multifold increases were also seen in A549 cancer cell lines and in Colo-205 cells. Reprogramming human primary cancer cells remains a challenge. We present data that even primary cancer cells show increases in the four Yamanaka factors. Metadichol treatment of triple-negative primary breast cancer (HCAF-TNPBC) primary cancer cells led to increases in OKSM factors by 19.6, 8.07, 2.45, and 6.91 times in concentration ranges of 1 picogram to 100 nanograms. Metadichol induces the expression of Klotho, an antiaging gene, that curbs the expression of the TP53 gene, as this is a pre-requisite for reprogramming somatic cells into iPSCs. Metadichol also increases endogenous vitamin C levels, which is necessary for the efficient reprogramming of somatic cells into iPSCs. Metadichol an extract from food that is nontoxic, which opens the door to direct testing in humans with the potential for applications in stem cell therapy
Combustion of Al nanoparticles coated with NC/ethanol/ether molecules by equilibrium molecular dynamics simulations
Authors: Jin Xin , Wang Lei , Liu Pingan , Sui Penghua
Published: June 2022

Al nano-particle (ANP) have high reactivity, but they are easily inactivated by external oxidants. To improve its surface property, we coat ANPs with nitrocellulose (NC)/ethanol/ether solution. And MD simulation method is used to simulate the coating, ignition and combustion processes of ANPs under three different coating conditions. Our results show that the NC/ethanol/ether formed a dense coating layer on the surface of annealed ANPs and passivated ANPs through physical and chemical adsorption. The coating layer can block the contact between the active Al atoms and O2 molecules at low temperature. In ignition phase, NC/ethanol/ether coating layer can increase the density of O2 molecules around ANPs and the surface temperature of ANPs. At the same time, due to the desorption and diffusion of the coating layer exposed more reaction sites, ANPs have shorter ignition delay and lower ignition temperature. According to the change of atomic displacement, the combustion stage can be divided into three stages. The NC molecules can increase the combustion propagation speed, combustion time and efficiency of ANPs. Such improvement will enable ANPs to obtain better storage performance, combustion performance and play a stronger role in the field of energetic materials.
Latent factors affecting safer injection practices that reduce infection and how education can improve them
Authors: Park Jung Wan , Park Samel , Lee Eunjung , Kim Tark , Kim Eu Suk , Kim Bongyoung , Yoo So Yeon , Han Su Ha , Kim Tae Hyong
Published: June 2022

Background
The incidence of healthcare-associated infections, particularly injection-related infections, increases patient comorbidities even in countries with adequate medical resources. Although there are clear guidelines for injection practices to prevent infection, their application in clinical settings is insufficient. Therefore, we have identified factors that effectively affect the application of injection practices with reduced infection.
Methods
We developed a questionnaire related to safe injection for infection prevention and administered it to the medical staff in charge of injection practice. The survey questionnaires were classified into four factors through factor loading, and the factors affecting injection practice performance were analyzed using a multivariable robust regression test.
Results
In total, 842 questionnaires were analyzed. Each questionnaire was classified into four factors: “Reuse and contamination,” “Compliance with aseptic technique,” “Exchange of infusion set,” and “Use of multidose vials,” Nurses with higher careers showed more compliance; education within one year and awareness of each item of the questionnaire had positive associations with proper injection practice.
Conclusions
The most important factor in the proper injection practice with reduced infection is education, and relevant knowledge through timely education positively affects the performance and compliance related to safe injection.
Increase in COVID-19 downwind following a wind change
Authors: Hailwood Alice , Graham Catriona , Ramalingam Sandeep
Published: June 2022

Aerosolized SARS COV-2 is viable for at least 3–5 hours. Aerosols can rapidly become droplet nuclei and be carried long distances by wind before they settle. We hence investigated the possibility of identifying wind-assisted transmission of COVID-19. COVID-19 cases/100,000 population was calculated for hotspots and surrounding areas. Daily wind direction/speed data for hotspots was collated. Seven-day rolling averages of COVID-19 cases/100,000 population was plotted against wind direction/speed to compare case rate trends in hotspots, upwind and downwind areas. Within 14 days of the wind blowing into an area, case rate trends downwind differed from that of the hotspot. The difference compared to the hotspot could be an increase, plateau, or a slower decrease. This suggests that viral particles carried by the wind can lead to an increase in infections downwind. Research on ways to reduce aerosol transmission in the community is urgently needed.
An exosome-related long non-coding RNAs risk model could predict survival outcomes in patients with breast cancer
Authors: Qiu Pengjun , Guo Qiaonan , Lin Jianqing , Pan Kelun , Chen Jianpeng , Ding Mingji
Published: June 2022

Background: Breast cancer (BC) is one of the most frequent malignancies among women worldwide. Accumulating evidence indicates that long non-coding RNA (lncRNA) may affect BC progression. Exosomes, a class of small membrane vesicles, have been reported to promote tumor progression through transporting proteins, mRNAs, lncRNAs and so on. However, the interaction between exosome-related lncRNAs and the microenvironment of malignancies is unclear. Hence, we proceeded to investigate the relationship between exosome-related lncRNAs and BC microenvironment. Method: 121 exosome-associated genes were extracted from ExoBCD database. Then, the Pearson analysis was used to screened out the exosome-related lncRNAs. After that, 15 exosome-related differentially expressed lncRNAs were identified by the correlation with BC prognosis. According to the sum of the expression of these 15 lncRNAs, extracted from The Cancer Genome Atlas (TCGA), and the regression coefficients, an exosome-related lncRNAs signature was developed by using Cox regression analysis. With the median risk score of the training set, the patients in training and validation sets were separated to low-risk group and high-risk group. Subsequently, the lncRNA–mRNA co-expression network was constructed. The distinct enrichment pathways were compared among the different risk groups by using the R package clusterProfiler. The ESTIMATE method and ssGESA database were adopted to study the ESTIMATE Score and immune cell infiltration. Eventually, the expression of immune checkpoint associated genes, microsatellite instable (MSI) and the immunophenoscore (IPS) were further analyzed between different risk groups. Results: Different risk groups exhibited different prognosis, with lower survival rate in the high-risk group. The differentially expressed genes (DEGs) between the different risk groups were enriched in biological processes pathways as well as immune responses. BC patients in high-risk group were identified with lower scores of ESTIMATE scores. Subsequently, we noticed that the infiltrating levels of aDCs, B cells, CD8+ T cells, iDCs, DCs, Neutrophils, macrophages, NK cells, pDCs, Tfh, T helper cells, TIL and Tregs were obvious elevated with the decreased risk score in training and validation cohorts. And some immune signatures were significantly activated with the decreased risk score in both cohorts. Eventually, the exosome-associated lncRNAs risk model was demonstrated to accurately predict immunotherapy response in patients with BC. Conclusion: The results of our study suggest that exosome-related lncRNAs risk model has close relationship with prognosis and immune cells infiltration in BC patients. These findings could make a great contribution to improving BC immunotherapy.
Gene Expression Alterations in Peripheral Blood Following Sport-Related Concussion in a Prospective Cohort of Collegiate Athletes: A Concussion Assessment, Research and Education (CARE) Consortium Study
Authors: Liu Yunlong , Simpson Edward , Nudelman Kelly , Riggen Larry , Menser Michael , Ren Jie , Reiter Jill , Harezlak Jaroslaw , Foroud Tatiana , Saykin Andrew , Broglio Steve , McCrea Michael , Pasquina Paul , McAllister Thomas
Published: June 2022

Rapid diagnosis of concussion is essential to effective treatment and recovery. Concussion biomarker research has focused primarily on blood-based protein assays to detect markers of brain injury. However, transcriptomic data provides insight into the complex biological response to concussion. In this study, we investigated RNA-seq transcriptome analysis of whole blood in a large cohort of concussed and control collegiate athletes who were participating in the Concussion Assessment, Research and Education (CARE) Consortium. In this multicenter prospective cohort study, blood samples were collected from collegiate athletes at preseason (baseline), post-injury (0–6 hours), 24–48 hours postinjury, time of symptom resolution, 7 days after unrestricted return to play and 6 months post-injury. RNA-sequencing was performed on samples from 230 concussed, 130 contact control and 102 non-contact control athletes. Differential gene expression analysis was performed at each timepoint relative to baseline. Deconvolution analysis was used to identify differences in immune cell types. We identified key genes and pathways that were activated in response to concussion. Cytokine and immune response signaling pathways were activated immediately after concussion, but at later time points, these pathways appeared to be suppressed relative to contact controls. RNA-seq data also revealed that the proportion of neutrophils increased and natural killer cells decreased in the blood following concussion. Transcriptome signatures in the blood reflect the known pathophysiology of concussion and may be useful for defining the immediate biological response and the time course for recovery. In addition, the identified immune response pathways and changes in immune cell type proportions following concussion could inform future treatment strategies.
The Use of Intercultural Interpreter Services at a Pediatric Emergency Department in Switzerland
Authors: Buser S , Gessler N , Gmuender M , Feuz U , Jachmann A , Fayyaz J , Keitel K , Brandenberger J
Published: June 2022

The aim of our study was to analyze the use of interpreter services and improve communication during health encounters with families with limited language proficiency (LLP) at the pediatric emergency department (ED) of the University Hospital of Bern.This study is a pre- and post-intervention study analyzing the use of interpreter services for LLP families. All families originating from a country with a native language other than German, English or French presenting to the ED were eligible to participate in the study. If they agreed to participate, the language proficiency of the caregiver present during the health encounter was systematically assessed during a phone interview within a few days after the consultation, using a standardized screening tool. If screened positive (relevant LLP), a second phone interview with an interpreter was conducted. Further variables were extracted including nationality, age, gender and date of visit using administrative health records. To increase the use of interpreter services, a package of interventions was implemented at the department during 3 months. It consisted of: i) in person and online transcultural teaching ii) awareness raising through the regular information channels and iii) the introduction of a pathway to systematically identify and manage LLP families. The proportion of LLP families who received an interpreter was 11.0% (14/127) in the pre-intervention period compared to 14.8% (20/135) in the post-intervention period. The interpreter use was therefore increased by 3.8% (95% CI -0.43 to 0.21; p = 0.36). The assessed level of language proficiency of caregivers differed from the self-reported level of language proficiency. Of the study participants in the interview whose language proficiency was screened as limited, 77.1% estimated their language proficiency level as intermediate. More than half of the LLP families who did not receive an interpreter and participated in the interview reported, that they would have liked an interpreter during the consultation. Conclusions: Interpreter services are largely underused during health encounters with LLP families. Relying on caregivers´ self-assessed language proficiency and their active request for an interpreter is not sufficient to ensure safe communication during health encounters. Systematic screening of language proficiency and standardized management of LLP families is feasible and needed at health care facilities to ensure equitable care. Further studies are needed to analyze personal and institutional barriers to interpreter use and find interventions to sustainably increase the use of interpreter services for LLP families.
Groundwater conceptual pollution model and related human health hazards, the main dilemma of a desert aquifer near ophiolite complex
Authors: Sudegi Peyman , Bagheri Rahim , Jafari Hadi , Qishlaqi Afshin
Published: June 2022

Groundwater is a finite resource in Davarzan region with limited annual rainfall in the northeast of Iran. This aquifer is located between the ophiolite complex mountain in the north and salty playa at the south. 26 groundwater and 4 springs’ samples were analyzed to assess the origin of groundwater pollution and explains links between the disturbed heavy metals composition of the earth’s surface and the human health risks. The main heavy metal pollutants in the groundwater are Cr, Fe, As and Pb ions. In general, the groundwater salinity and some elements such as Cr and As are increased along with surface topography and groundwater flow directions from the northern ophiolite highlands recharge area to the adjacent desert discharging zone in the south. Despite the ophiolite complexes being the most enriched in Cr element, the lowest Cr concentration in the groundwater was measured near the ophiolite area, which is in the range of its discharged springs. Based on the groundwater conceptual pollution model, bedrock geochemistry controls the composition of soil and hence that of groundwater. The Cr samples show a direct relation with the EC value indicating that intrusion of salinity from the salt pan is probably another reason for the increased Cr concentration. They are the most important natural sources of metal entry into bodies of the groundwater. The results of health risk assessment indicated that the groundwater suffered from significant contamination and if used for long-term without pre-treatment, may pose serious health risks to human population via drinking water and irrigation of agricultural fields. This is the first attempt to apply hydrogeological setting along with the source of pollution and its health risk in a desert-ophiolitic area.
Protein nanobarcodes enable single-step multiplexed fluorescence imaging
Authors: Bolm Daniëlle de Jong - , Sadeghi Mohsen , Bao Guobin , Klaehn Gabriele , Hoff Merle , Mittelmeier Lucas , Basamanav Buket , Opazo Felipe , Noé Frank , Rizzoli Silvio
Published: June 2022

Multiplexed cellular imaging typically relies on the sequential application of detection probes, such as antibodies or DNA barcodes, which is complex and time-consuming. To address this, we developed here protein nanobarcodes, composed of combinations of epitopes recognized by specific sets of nanobodies. The nanobarcodes are read in a single imaging step, relying on nanobodies conjugated to distinct fluorophores, which enables a precise analysis of large numbers of protein combinations.
Effectiveness of brief psychosocial support on mental health of cancer patients and their relatives: A non-randomized controlled evaluation of cancer counseling centers
Authors: Lingens Solveigh P. , Schilling Georgia , Schulz Holger , Bleich Christiane
Published: June 2022

Purpose: Patients with cancer and their relatives often experience a deterioration of mental health while dealing with the consequences of the disease. The aim of the present study was to evaluate changes of mental health during and after brief psychosocial support at cancer counselling centers.Methods: In this non-randomized controlled trial n = 885 participants were recruited and n = 449 were eligible for the analysis. The intervention group (IG, n = 264) was recruited at two cancer counselling centers in Germany. The control group (CG, n = 195) were cancer patients or relatives who did not seek support at one of the aforementioned cancer counseling centers. To compare differences between IG and CG across three points in time (before the first counseling session and subsequently after two twelve weeks), a linear mixed model analysis (LLM) was performed. The primary outcome was distress; secondary outcomes were anxiety and depressive symptoms, well-being, cancer specific and generic quality of life (QoL), self-efficacy, and fatigue. Results: The LLM showed significant differences between IG and CG at follow-up for distress (d = 0.36), depressive (d = 0.22) and anxiety symptoms (d = 0.22), well-being (d = 0.26), quality of life (QoL mental, d = 0.26), cancer specific QoL (symptoms, d = 0.13) and QoL (global, d = 0.27). The changes were not significant for QoL (physical), cancer specific QoL (functional) and fatigue. Conclusion: The results suggest that brief psychosocial support is associated with the improvement of mental health of cancer patients and their relatives after three months.The study was approved by the Center of Psychosocial Medicine (LPEK) at the University Clinic Hamburg-Eppendorf on 22nd of August 2018 (No: LPEK-007). The study was registered prior to data collection with the German Registration of Clinical Trials (DRKS, ID: DRKS00015516) in September 2018.
Internationalization of Medical Education in China: An Investigation of Medical Graduates’ Global Competence and its influencing factors
Authors: Jiang Qin , Song Xiaokang , Wang Ke , Yue Cailing , Sun Hong , Zhang Zhuoqi , Hu Bin , Li Hui , Miao Gugen , Miao Chunxia , Duan Wei , Liu Shenjun
Published: June 2022

Global competence has become an important competence for medical graduates in the globalized world. However, no research has been made to measure medical graduates’ global competence. To fill the gap, this study was built upon the scholarship published in the field and administered the questionnaire to China medical graduates sampled from four institutions of medical education. We found that the sample had a relatively good level of global competence in general, but lacked skills in cross-cultural communication and international academic communication. We also found that “Internationalization Concept and System” and “International Development of Teachers” in the school dimension and “Taking International Courses”, “International Publication” and “Participation in International Conference” in the dimension of individual international involvement had a significant positive impact on the cultivation of global competence. In conclusion, the universities should aim for the construction of an effective institutional mechanism for internationalization to help improve students’ global competence.
Perinodular and intranodular radiomic features on 18F-FDG PET/CT images predict PD-L1 status in non-small cell lung cancer
Authors: Wang Yubo , He Xinghua , Song Xiaoyi , Li Man , Zhu Ding , Zhang Fanwei , Chen Qingling , Lu Yao , Wang Ying
Published: June 2022

Background
Programmed death-ligand 1 (PD-L1) status can affect the efficacy of immunotherapy. Based on the link between PD-L1 expression and metabolism, we hypothesize that radiomics based on 18F-FDG PET/CT can provide clinical decision support for patients with non-small cell lung cancer (NSCLC).
Methods
In this retrospective study, 18F-FDG PET/CT images and clinical data of 394 eligible patients were divided into training (n = 315) and test sets (n = 79). Radiomic features of the CT and PET images were extracted and these were analysed using five different machine learning classifiers. Combining semantic features with the best radiomic model, a clinical-radiomic model was established to predict the PD-L1 status in patients with NSCLC.
Results
The radiomic model based on PET intranodular features preform the best, yielding an area under receiver operating characteristics curve (AUC) of 0.791 (95% CI: 0.788, 0.799) on the test set. By adding semantic features, the test set AUC improved to 0.795 (95% CI: 0.794, 0.803).
Conclusion
This study showed that 18F-FDG PET/CT-based radiomics could be used as a non-invasive biomarker to provide more accurate and personalized decision support for patients with NSCLC.
Seismic hazard maps of the eastern Himalaya syntaxis by integrating the surface topography and site effects
Authors: Rahman M. Moklesur , Bai Ling
Published: June 2022

The complex tectonic position and the long history of the devastating earthquakes with massive casualties and socio-economic damages have highlighted the importance of proper seismic hazard assessment for the eastern Himalaya syntaxis region. Here, probabilistic seismic hazards are computed combining the area, linear and smooth grid seismogenic source models. The surface topography and the site effects are incorporated to thousands of 0.1° x 0.1° grid cells. Uniform moment magnitude of declustered catalogs are used. The mean seismicity rate, the b-value and the expected maximum magnitude are estimated using the maximum likelihood algorithm. Two sets of ground motion prediction equations for shallow crustal and subduction interface tectonic regime are assigned to every source model by utilizing a logic tree to reduce the epistemic uncertainties in hazard calculation. The peak ground acceleration and spectral acceleration are estimated at 0.2s and 1.0s for 2% and 10% probabilities of exceedance in 50 years. The hazard maps show a wide range of spatio-temporal variation of values. The topographic irregularities and site effects significantly amplified the seismic hazard values. High hazardous zones are associated with the continental plate collision beneath the Assam Syntaxis. Medium hazard is estimated at Namche Barwa syntaxis. This analysis suggests the integration of actual site conditions, well developed ground motion prediction equations and multiple source models through logic tree to evaluate the better seismic hazards for any complex region.
Surgical treatment of recurrent retroperitoneal sarcoma in its different patterns: a 15-years’ two-centers experience
Authors: Guadagni Simone , Peri Andrea , Palmeri Matteo , Furbetta Niccolò , Franco Gregorio Di , Filardo Matteo , Comandatore Annalisa , Pollina Luca Emanuele , Gaeta Raffaele , Sbrana Andrea , Galli Luca , Mercinelli Chiara , Pietrabissa Andrea , Candio Giulio Di , Morelli Luca
Published: June 2022

Introduction:
Few data are available about the role of surgery in the treatment of retroperitoneal sarcoma (RPS) recurrences. We herein report the short- and mid-term outcomes of patients who underwent surgical treatment of RPS recurrences at two Italian centers.
Materials and Methods
From January 2005 to January 2020, 33 patients underwent surgical treatment of isolated locally recurrent RPS (LR group), locally recurrent RPS associated with the presence of distant recurrence (LR + DM group), and distant-only recurrent RPS (DM group). Data regarding pre-, intra-, post-operative course, and follow-up, were retrospectively analyzed and compared.
Results
LR-group was composed of 15 patients, LR + DM group of 9 patients, and DM group of 9 patients. During the follow-up, 78.5% of the LR group, 77.8% of the DM group and 100% of the LR + DM group (p = 0.244) experienced a second recurrence. No differences in the mean disease-free survival (p = 0.127), overall survival (OS) (p = 0.165) was reported between the three groups. Repeated surgery was an independent factor affecting survival in multivariate analysis (p = 0.01).
Conclusions
A surgical treatment of RPS recurrences should always be taken into consideration, also in metastatic patients and/or in those who have already undergone surgery, because this approach may offer longer survival.