bluuebunny/crossref_metadata_2025 huggingface dataset:
Forecasting Charging Point Occupancy Using Supervised Learning Algorithms
Authors: Ostermann Adrian , Fabel Yann , Ouan Kim , Koo Hyein
Published: May 2022

The prediction of charging point occupancy enables electric vehicle users to better plan their charging processes and thus promotes the acceptance of electromobility. The study uses Adaptive Charging Network data to investigate a public and a workplace site for predicting individual charging station occupancy as well as overall site occupancy. Predicting individual charging point occupancy is formulated as a classification problem, while predicting total occupancy is formulated as a regression problem. The effects of different feature sets on the predictions are investigated, as well as whether a model trained on data of all charging points per site performs better than one trained on the data of a specific charging point. Reviewed studies so far, however, have failed to compare these two approaches to benchmarks, to use more than one algorithm, or to consider more than one site. Therefore, the following supervised machine-learning algorithms were applied for both tasks: linear and logistic regression, k-nearest neighbor, random forest, and XGBoost. Further, the model results are compared to three different naïve approaches which provide a robust benchmark, and the two training approaches were applied to two different sites. By adding features, the prediction quality can be increased considerably, which resulted in some models performing better than the naïve approaches. In general, models trained on data of all charging points of a site perform slightly better on median than models trained on individual charging points. In certain cases, however, individually trained models achieve the best results, while charging points with very low relative charging point occupancy can benefit from a model that has been trained on all data.
An Application of Kolmogorov Complexity and Its Spectrum to Positive Surges
Authors: Gualtieri Carlo , Mihailović Anja , Mihailović Dragutin
Published: May 2022

A positive surge is associated with a sudden change in flow that increases the water depth and modifies flow structure in a channel. Positive surges are frequently observed in artificial channels, rivers, and estuaries. This paper presents the application of Kolmogorov complexity and its spectrum to the velocity data collected during the laboratory investigation of a positive surge. Two types of surges were considered: a undular surge and a breaking surge. For both surges, the Kolmogorov complexity (KC) and Kolmogorov complexity spectrum (KCS) were calculated during the unsteady flow (US) associated with the passage of the surge as well as in the preceding steady-state (SS) flow condition. The results show that, while in SS, the vertical distribution of KC for Vx is dominated by the distance from the bed, with KC being the largest at the bed and the lowest at the free surface; in US only the passage of the undular surge was able to drastically modify such vertical distribution of KC resulting in a lower and constant randomness throughout the water depth. The analysis of KCS revealed that Vy values were peaking at about zero, while the distribution of Vx values was related both to the elevation from the bed and to the surge type. A comparative analysis of KC and normal Reynold stresses revealed that these metrics provided different information about the changes observed in the flow as it moves from a steady-state to an unsteady-state due to the surge passage. Ultimately, this preliminary application of Kolmogorov complexity measures to a positive surge provides some novel findings about such intricate hydrodynamics processes.
Cereal and Confectionary Packaging: Assessment of Sustainability and Environmental Impact with a Special Focus on Greenhouse Gas Emissions
Authors: Krauter Victoria , Bauer Anna-Sophia , Milousi Maria , Dörnyei Krisztina Rita , Ganczewski Greg , Leppik Kärt , Krepil Jan , Varzakas Theodoros
Published: May 2022

The usefulness of food packaging is often questioned in the public debate about (ecological) sustainability. While worldwide packaging-related CO2 emissions are accountable for approximately 5% of emissions, specific packaging solutions can reach significantly higher values depending on use case and product group. Unlike other groups, greenhouse gas (GHG) emissions and life cycle assessment (LCA) of cereal and confectionary products have not been the focus of comprehensive reviews so far. Consequently, the present review first contextualizes packaging, sustainability and related LCA methods and then depicts how cereal and confectionary packaging has been presented in different LCA studies. The results reveal that only a few studies sufficiently include (primary, secondary and tertiary) packaging in LCAs and when they do, the focus is mainly on the direct (e.g., material used) rather than indirect environmental impacts (e.g., food losses and waste) of the like. In addition, it is shown that the packaging of cereals and confectionary contributes on average 9.18% to GHG emissions of the entire food packaging system. Finally, recommendations on how to improve packaging sustainability, how to better include packaging in LCAs and how to reflect this in management-related activities are displayed.
A High-Luminescence Biomimetic Nanosensor Based on N, S-GQDs-Embedded Zinc-Based Metal–Organic Framework@Molecularly Imprinted Polymer for Sensitive Detection of Octopamine in Fermented Foods
Authors: Guo Ying , Yuan Guanqing , Hu Xuelian , Zhang Jinni , Fang Guozhen
Published: May 2022

In this study, a novel fluorescent molecularly imprinted nanosensor (N, S-GQDs@ZIF-8@MIP) based on the nitrogen and sulfur co-doped graphene quantum dots decorated zeolitic imidazolate framework-8 was constructed for the detection of octopamine (OA). Herein, ZIF-8 with a large surface area was introduced as a supporter of the sensing system, which effectively shortened the response time of the sensor. Meanwhile, high green luminescent N, S-GQDs and a maximum emission wavelength of 520 nm under 460 nm excitation and a 12.5% quantum yield were modified on the surface of ZIF-8 as a signal tag that can convert the interactions between the sensor and OA into detectable fluorescent signals. Finally, N, S-GQDs@ZIF-8@MIP was acquired through the surface molecular imprinting method. Due to the synergy of N, S-GQDs, ZIF-8, and MIP, the obtained sensor not only demonstrated higher selectivity and sensitivity than N, S-GQDs@ZIF-8@NIP, but also displayed faster fluorescence response than N, S-GQDs@MIP. Under optimal conditions, the developed sensor presented a favorable linear relationship in the range of 0.1–10 mg L−1 with a detection limit of 0.062 mg L−1. Additionally, the proposed N, S-GQDs@ZIF-8@MIP strategy was effectively applied to the detection of OA in fermented samples, and the obtained results had a satisfactory correlation with those of HPLC.
Phytochemical Analysis, α-Glucosidase and α-Amylase Inhibitory Activities and Acute Toxicity Studies of Extracts from Pomegranate (Punica granatum) Bark, a Valuable Agro-Industrial By-Product
Authors: Laaraj Nassima , Bouhrim Mohamed , Kharchoufa Loubna , Tiji Salima , Bendaha Hasnae , Addi Mohamed , Drouet Samantha , Hano Christophe , Lorenzo Jose Manuel , Bnouham Mohamed , Mimouni Mostafa
Published: May 2022

Punica granatum is a tree of the Punicaceae family which is widespread all over the world with several types of varieties. Its fruit juice is highly prized, whereas the bark, rich in in phytochemicals such as flavonoids, hydrolysable tannins, phenolic acids, and fatty acids, is regarded an agro-industrial waste. It is utilized in traditional medicine for its medicinal properties in the treatment and prevention of a variety of ailments. This study aims to extract and to separate the phytochemical compounds from the bark of P. granatum, to identify them and to study the inhibitory effect of its extracts against antidiabetic activity. First, we carried out successive hot extractions with solvents (chloroform, acetone, methanol, and water) of increasing polarity by the Soxhlet. Then, using both qualitative and quantitative phytochemical investigation, we were able to identify groups of chemicals that were present in all extracts. We identified the majority of the molecular structures of chemicals found in each extract using HPLC-DAD analysis. The inhibition against both intestinal α-glucosidase and pancreatic α-amylase enzymes by P. granatum extracts was used to evaluate their potential antidiabetic effect in vitro. Our results demonstrated the great potential of the acetone extract. Ellagic acid, (−)-catechin, vanillin and vanillic acid were proposed as the most active compounds by the correlation analysis, and their actions were confirmed through the calculation of their IC50 and the determination of their inhibition mechanisms by molecular modelling. To summarize, these results showed that P. granatum bark, a natural agro-industrial by-product, may constitute a promising option for antidiabetic therapeutic therapy.
A Simplified Approach to Understanding Body Cooling Behavior and Estimating the Postmortem Interval
Authors: Sharma Pushpesh , Kabir C. S.
Published: May 2022

Ascertaining the postmortem interval or PMI has been an item of interest over many decades in forensic science for crime scene investigations. The challenge revolves around establishing the postmortem interval or PMI with a single temperature measurement, given the known initial and the final boundary condition of a human body and room temperature. Despite the advent of a succession of single, double, and triple-exponential analytical models, and more recently, the 3-D heat-transfer modeling, the uncertainty remains in the PMI estimation. This study presents a pragmatic way to solve this problem in a two-step approach. First, we attempted to understand the cooling rate in various body parts. Second, we proposed a hyperbolic modeling approach to fit the time-dependent temperature data to estimate the PMI. The latest digital data of Wilk et al.’s study provided the platform for validating our solution approach. Overall, the use of 20 subsets of three bodies involving Wilk et al. and five from one body of Bartgis et al. provided the required data. Although body imaging and 3-D modeling greatly facilitate our understanding of overall body-cooling behavior in the modern era in real-time, a simple semi-analytical tool can corroborate the model results for PMI.
Initial Analysis of Structural Variation Detections in Cattle Using Long-Read Sequencing Methods
Authors: Gao Yahui , Ma Li , Liu George E.
Published: May 2022

Structural variations (SVs), as a great source of genetic variation, are widely distributed in the genome. SVs involve longer genomic sequences and potentially have stronger effects than SNPs, but they are not well captured by short-read sequencing owing to their size and relevance to repeats. Improved characterization of SVs can provide more advanced insight into complex traits. With the availability of long-read sequencing, it has become feasible to uncover the full range of SVs. Here, we sequenced one cattle individual using 10× Genomics (10 × G) linked read, Pacific Biosciences (PacBio) continuous long reads (CLR) and circular consensus sequencing (CCS), as well as Oxford Nanopore Technologies (ONT) PromethION. We evaluated the ability of various methods for SV detection. We identified 21,164 SVs, which amount to 186 Mb covering 7.07% of the whole genome. The number of SVs inferred from long-read-based inferences was greater than that from short reads. The PacBio CLR identified the most of large SVs and covered the most genomes. SVs called with PacBio CCS and ONT data showed high uniformity. The one with the most overlap with the results obtained by short-read data was PB CCS. Together, we found that long reads outperformed short reads in terms of SV detections.
USP10 as a Potential Therapeutic Target in Human Cancers
Authors: Tao Li , Liu Xiao , Jiang Xinya , Zhang Kun , Wang Yijing , Li Xiumin , Jiang Shulong , Han Tao
Published: May 2022

Deubiquitination is a major form of post-translational protein modification involved in the regulation of protein homeostasis and various cellular processes. Deubiquitinating enzymes (DUBs), comprising about five subfamily members, are key players in deubiquitination. USP10 is a USP-family DUB featuring the classic USP domain, which performs deubiquitination. Emerging evidence has demonstrated that USP10 is a double-edged sword in human cancers. However, the precise molecular mechanisms underlying its different effects in tumorigenesis remain elusive. A possible reason is dependence on the cell context. In this review, we summarize the downstream substrates and upstream regulators of USP10 as well as its dual role as an oncogene and tumor suppressor in various human cancers. Furthermore, we summarize multiple pharmacological USP10 inhibitors, including small-molecule inhibitors, such as spautin-1, and traditional Chinese medicines. Taken together, the development of specific and efficient USP10 inhibitors based on USP10’s oncogenic role and for different cancer types could be a promising therapeutic strategy.
Numerical Modelling Challenges in Rock Engineering with Special Consideration of Open Pit to Underground Mine Interaction
Authors: Shapka-Fels Tia , Elmo Davide
Published: May 2022

This paper raises important questions about the way we approach numerical analysis in rock engineering design. The application of advanced numerical models is essential to adequately analyze and design different geotechnical aspects of pit-to-cave transitions. We present a critical review of numerical methods centered around the hypothesis that a model is not, and cannot be, a perfect imitation of reality; therefore, numerical modelling of large-scale mining projects requires the real problem to be idealized and simplified. The discussion highlights the dichotomy of continuum vs. discontinuum modelling and the important question of whether continuum models can effectively capture dynamic continuum-to-discontinuum processes typical of cave mining. The discussion is complemented by examples of hybrid continuum-discontinuum models to analyze the important problem of transitioning from surface (open pit) mining to underground mass mining (caving). The results demonstrate the hypothesis that forward modelling should be performed in the context of a risk-based approach, with numerical models becoming investigative tools to assess risk and evaluate the impact of different unknowns, thus classifying modelling outputs in terms of expected consequences.
A Chronological Database about Natural and Anthropogenic Sinkholes in Italy
Authors: Vennari Carmela , Parise Mario
Published: May 2022

Sinkholes are a widespread geological hazard, typical of karst lands, where they generally originate as collapse features related to presence of underground voids. Nevertheless, other types of sinkholes can be formed through solution, suffusion and sagging processes. Sinkholes can also be originated in relation to artificial cavities, excavated by man in past times. In Italy, sinkholes interest large sectors of the country, given the very long history of Italy with an intense utilization of the underground. They cause serious damage to infrastructures, economic activities, and human health every year. We present a catalogue on natural and anthropogenic sinkholes in Italy, as the first step toward evaluation of the sinkhole hazard. After introducing sinkholes, which is definitely a highly underrated type of disaster in Italy, we point out their occurrence in the country. We illustrate the methodology used to build the database, with particular focus on accuracy and reliability of the data. Collecting information from different types of sources, a catalogue of some 1190 sinkhole events is built. Database structure and data analysis are then illustrated. Eventually, we draw some conclusions on the likely uses of our work by providing recommendations for environmental management on this very delicate issue.
Assessment of Traffic Accidents in Japan during the COVID-19 Pandemic vs. Previous Years: A Preliminary Report
Authors: Shimizu Kazuki , Ueda Peter , Ghaznavi Cyrus , Sakamoto Haruka , Nomura Shuhei
Published: May 2022

Beginning in April 2020, social distancing measures were implemented to mitigate the COVID-19 pandemic in Japan. We assessed whether traffic accident rates had decreased from April 2020 to December 2021 as compared with previous years. The analysis included 2,934,477 traffic accidents, and the trend of decreasing rates of traffic accidents in recent years and seasonal fluctuations in traffic accidents were considered. The yearly change in the traffic accident rate between 2015 and 2019 was estimated, and the traffic accident rate in 2020 and 2021 was predicted. This was followed by the comparison of observed vs. predicted traffic accident rate. In 2020, the observed vs. expected rates of traffic accidents were lower in April to December 2020, and the rate of traffic accidents in Japan was 30–40% lower in April–May 2020 than would be expected based on trends from previous years. In 2021, rates of traffic accidents remained lower than expected between January and November, but the magnitude of decrease was not as pronounced. These findings could be explained by social distancing policies, including the declaration of the state of emergency, and the relaxation of public health and social measures over time.
Associations between Fear of COVID-19, Depression, and Internet Addiction in South Korean Adults
Authors: Lee Jung Jae , Shin Sun-Hwa
Published: May 2022

This study investigates the associations between Internet addiction and psychological distress, including fear of coronavirus disease 2019 (COVID-19), and depression in South Korean adults during the COVID-19 pandemic. A population-based cross-sectional online survey was conducted from 14 to 18 May 2021. Potential adult survey respondents aged 20 years or older were randomly extracted from one of the largest online survey panels in South Korea, matching the South Korean national demographic proportions (i.e., region, gender, and age). Subsequently, 1155 participants were included in the study. Descriptive and logistic regression analyses were performed to investigate the participants’ characteristics and analyze the adjusted odds ratios (aORs). Of the South Korean adults, 11.17%, 15.15%, and 37.23% were at risk of Internet addiction, COVID-19 fear, and depression, respectively. Internet addiction was positively associated with COVID-19 fear (aOR = 3.25, 95% CI [2.10, 5.04]) and depressive symptoms (aOR = 6.40, 95% CI [4.15, 9.86]). Addictive Internet use was significantly associated with psychological distress during the COVID-19 pandemic. The development of public health strategies that mitigate addictive Internet use and increase eHealth literacy will be useful for securing the public’s psychological well-being.
The Effects of Combined Physical and Cognitive Interventions on Direct and Indirect Fall Outcomes for the Elderly with Mild Cognitive Impairment: A Systematic Review
Authors: Mai Ba Hai , Kim Jiyun
Published: May 2022

This review was intended to determine the effectiveness of physical and cognitive training (PCT) on falls and fall-related factors and cognitive function among community-dwelling elderly people with mild cognitive impairment (MCI). A systematic literature search was performed of the MEDLINE, CINAHL, Web of Sciences, Scopus, ProQuest, Embase, and Google Scholar databases for articles published from 2010 to 2020. The studies that combined PCT to assess their impacts on fall outcomes both directly and indirectly were included. Study quality was assessed using the standardized JBI Critical Appraisal Tool for RCTs. The standardized data extraction tool from JBI-MAStARI was used to extract data of included studies. Seven RCTs involving 740 participants were included. The overall fall incidence did not significantly decrease after the interventions. However, PCT significantly impacted the cognitive function and physical activities of elderly people with MCI, particularly improving their balancing ability, gait speed, muscular strength, and executive functions. This study indicated that combining PCT improves balance ability, gait speed, and executive functioning in the elderly with MCI, which may help to minimize fall occurrence.
Educational Intervention in the Postural Hygiene of School-Age Children
Authors: Menor-Rodríguez María José , Rodríguez-Blanque Raquel , Montiel-Troya María , Cortés-Martín Jonathan , Aguilar-Cordero María José , Sánchez-García Juan Carlos
Published: May 2022

Introduction: Healthy lifestyle habits formed in childhood provide the foundation for a healthy adult life; therefore, it is important to encourage healthy habits and to correct poor habits from an early age. Aim: In this study, we aim to evaluate the influence of educational intervention in order to modify postural hygiene habits in school-age children. Materials and methods: We randomly selected three public primary education schools in the Galician provinces of Orense and Pontevedra based on stratified multistage sampling. A sample of 479 students was obtained, representing 2% of all 6- to 12-year-old children registered during the 2015/2016 academic year in those provinces. Results: Following the intervention, the students’ postural hygiene improved. We found statistically significant differences regarding a reduction in the number of hours spent watching television (0.531 h/day); the way school books and supplies were carried, with an increase in the use of backpacks with wheels (from 58.5% to 64.1%); and an improvement in postural hygiene when watching television, with an increase from 63.7% to 80.8% of those surveyed opting to watch television whilst seated on a chair instead of lying down. Conclusions: Educational intervention by inculcating healthy postural hygiene habits in children at an early age can improve and correct unhealthy behaviours.
Tissue Transglutaminase Knock-Out Preadipocytes and Beige Cells of Epididymal Fat Origin Possess Decreased Mitochondrial Functions Required for Thermogenesis
Authors: Lénárt Kinga , Bankó Csaba , Ujlaki Gyula , Póliska Szilárd , Kis Gréta , Csősz Éva , Antal Miklós , Bacso Zsolt , Bai Péter , Fésüs László , Mádi András
Published: May 2022

Beige adipocytes with thermogenic function are activated during cold exposure in white adipose tissue through the process of browning. These cells, similar to brown adipocytes, dissipate stored chemical energy in the form of heat with the help of uncoupling protein 1 (UCP1). Recently, we have shown that tissue transglutaminase (TG2) knock-out mice have decreased cold tolerance in parallel with lower utilization of their epididymal adipose tissue and reduced browning. To learn more about the thermogenic function of this fat depot, we isolated preadipocytes from the epididymal adipose tissue of wild-type and TG2 knock-out mice and differentiated them in the beige direction. Although differentiation of TG2 knock-out preadipocytes is phenotypically similar to the wild-type cells, the mitochondria of the knock-out beige cells have multiple impairments including an altered electron transport system generating lower electrochemical potential difference, reduced oxygen consumption, lower UCP1 protein content, and a higher portion of fragmented mitochondria. Most of these differences are present in preadipocytes as well, and the differentiation process cannot overcome the functional disadvantages completely. TG2 knock-out beige adipocytes produce more iodothyronine deiodinase 3 (DIO3) which may inactivate thyroid hormones required for the establishment of optimal mitochondrial function. The TG2 knock-out preadipocytes and beige cells are both hypometabolic as compared with the wild-type controls which may also be explained by the lower expression of solute carrier proteins SLC25A45, SLC25A47, and SLC25A42 which transport acylcarnitine, Co-A, and amino acids into the mitochondrial matrix. As a consequence, the mitochondria in TG2 knock-out beige adipocytes probably cannot reach the energy-producing threshold required for normal thermogenic functions, which may contribute to the decreased cold tolerance of TG2 knock-out mice.
Tissue Niches Formed by Intestinal Mesenchymal Stromal Cells in Mucosal Homeostasis and Immunity
Authors: Pasztoi Maria , Ohnmacht Caspar
Published: May 2022

The gastrointestinal tract is the largest mucosal surface in our body and accommodates the majority of the total lymphocyte population. Being continuously exposed to both harmless antigens and potentially threatening pathogens, the intestinal mucosa requires the integration of multiple signals for balancing immune responses. This integration is certainly supported by tissue-resident intestinal mesenchymal cells (IMCs), yet the molecular mechanisms whereby IMCs contribute to these events remain largely undefined. Recent studies using single-cell profiling technologies indicated a previously unappreciated heterogeneity of IMCs and provided further knowledge which will help to understand dynamic interactions between IMCs and hematopoietic cells of the intestinal mucosa. In this review, we focus on recent findings on the immunological functions of IMCs: On one hand, we discuss the steady-state interactions of IMCs with epithelial cells and hematopoietic cells. On the other hand, we summarize our current knowledge about the contribution of IMCs to the development of intestinal inflammatory conditions, such as infections, inflammatory bowel disease, and fibrosis. By providing a comprehensive list of cytokines and chemokines produced by IMCs under homeostatic and inflammatory conditions, we highlight the significant immunomodulatory and tissue niche forming capacities of IMCs.
Friend or Foe: The Relativity of (Anti)oxidative Agents and Pathways
Authors: Szarka András , Lőrincz Tamás , Hajdinák Péter
Published: May 2022

An element, iron, a process, the generation of reactive oxygen species (ROS), and a molecule, ascorbate, were chosen in our study to show their dual functions and their role in cell fate decision. Iron is a critical component of numerous proteins involved in metabolism and detoxification. On the other hand, excessive amounts of free iron in the presence of oxygen can promote the production of potentially toxic ROS. They can result in persistent oxidative stress, which in turn can lead to damage and cell death. At the same time, ROS—at strictly regulated levels—are essential to maintaining the redox homeostasis, and they are engaged in many cellular signaling pathways, so their total elimination is not expedient. Ascorbate establishes a special link between ROS generation/elimination and cell death. At low concentrations, it behaves as an excellent antioxidant and has an important role in ROS elimination. However, at high concentrations, in the presence of transition metals such as iron, it drives the generation of ROS. In the term of the dual function of these molecules and oxidative stress, ascorbate/ROS-driven cell deaths are not necessarily harmful processes—they can be live-savers too.
Coronary Artery Ectasia: Review of the Non-Atherosclerotic Molecular and Pathophysiologic Concepts
Authors: Richards Gavin H. C. , Hong Kathryn L. , Henein Michael Y. , Hanratty Colm , Boles Usama
Published: May 2022

Coronary artery ectasia (CAE) is frequently encountered in clinical practice, conjointly with atherosclerotic CAD (CAD). Given the overlapping cardiovascular risk factors for patients with concomitant CAE and atherosclerotic CAD, a common underlying pathophysiology is often postulated. However, coronary artery ectasia may arise independently, as isolated (pure) CAE, thereby raising suspicions of an alternative mechanism. Herein, we review the existing evidence for the pathophysiology of CAE in order to help direct management strategies towards enhanced detection and treatment.
Small Extracellular Vesicles Containing miR-34c Derived from Bone Marrow Mesenchymal Stem Cells Regulates Epithelial Sodium Channel via Targeting MARCKS
Authors: Hua Yu , Han Aixin , Yu Tong , Hou Yapeng , Ding Yan , Nie Hongguang
Published: May 2022

Epithelial sodium channel (ENaC) is a pivotal regulator of alveolar fluid clearance in the airway epithelium and plays a key role in the treatment of acute lung injury (ALI), which is mainly composed of the three homologous subunits (α, β and γ). The mechanisms of microRNAs in small extracellular vesicles (sEVs) derived from mesenchymal stem cell (MSC-sEVs) on the regulation of lung ion transport are seldom reported. In this study, we aimed at investigating whether miR-34c had an effect on ENaC dysfunction induced by lipopolysaccharide and explored the underlying mechanism in this process. Primarily, the effect of miR-34c on lung edema and histopathology changes in an ALI mouse model was investigated. Then the uptake of PKH26-labeled sEVs was observed in recipient cells, and we observed that the overexpression of miR-34c in MSC-sEVs could upregulate the LPS-inhibited γ-ENaC expression. The dual luciferase reporter gene assay demonstrated that myristoylated alanine-rich C kinase substrate (MARCKS) was one of target genes of miR-34c, the protein expression of which was negatively correlated with miR-34c. Subsequently, either upregulating miR-34c or knocking down MARCKS could increase the protein expression of phospho-phosphatidylinositol 3-kinase (p-PI3K) and phospho-protein kinase B (p-AKT), implying a downstream regulation pathway was involved. All of the above suggest that miR-34c in MSC-sEVs can attenuate edematous lung injury via enhancing γ-ENaC expression, at least partially, through targeting MARCKS and activating the PI3K/AKT signaling pathway subsequently.
Bispecific mAb2 Antibodies Targeting CD59 Enhance the Complement-Dependent Cytotoxicity Mediated by Rituximab
Authors: Stadlbauer Katharina , Andorfer Peter , Stadlmayr Gerhard , Rüker Florian , Wozniak-Knopp Gordana
Published: May 2022

Inhibition of complement activation via the overexpression of complement-regulatory proteins (CRPs), most notably CD46, CD55 and CD59, is an efficient mechanism of disguise of cancer cells from a host immune system. This phenomenon extends to counteract the potency of therapeutic antibodies that could lyse target cells by eliciting complement cascade. The manifold functions and ubiquitous expression of CRPs preclude their systemic specific inhibition. We selected CD59-specific Fc fragments with a novel antigen binding site (Fcabs) from yeast display libraries using recombinant antigens expressed in bacterial or mammalian cells. To produce a bispecific antibody, we endowed rituximab, a clinically applied anti-CD20 antibody, used for therapy of various lymphoid malignancies, with an anti-CD59 Fcab. This bispecific antibody was able to induce more potent complement-dependent cytotoxicity for CD20 and CD59 expressing Raji cell line measured with lactate dehydrogenase-release assay, but had no effect on the cells with lower levels of the primary CD20 antigen or CD20-negative cells. Such molecules are promising candidates for future therapeutic development as they elicit a higher specific cytotoxicity at a lower concentration and hence cause a lower exhaustion of complement components.
Effect of Temperature on the Adhesion and Bactericidal Activities of Ag+-Doped BiVO4 Ceramic Tiles
Authors: Zhang Ying , Zhao Xuhuan , Wang Hao , Fu Shiqi , Lv Xiulong , He Qian , Liu Rui , Ji Fangying , Xu Xuan
Published: May 2022

The aim of this research was to study the effect of temperature on the adhesion and disinfection activities of an Ag+-doped BiVO4 (Ag+/BiVO4) coating. Ag+/BiVO4 was prepared by a sol–gel method, and spraying was used as the deposition method of coating. X-ray diffraction patterns showed that the monoclinic scheelite phase of the samples was unchanged by annealing at 450–650 °C. Scanning electron microscopy results showed that, at high temperatures, the particles melted and formed a dense coating, and the roughness of the coating decreased after initially increasing. The adhesion and disinfection activities were evaluated by ASTM D3359-08 and disinfection experiments. The results showed that the samples modified by silver had a good disinfection activity when annealed in the range of 450–650 °C. The adhesion increased upon increasing the annealing temperature. The sample annealed at 650 °C showed the best coating adhesion and completely killed Escherichia coli, Staphylococcus aureus, Shigella, and Salmonella after 2 h of visible-light irradiation.
The Impact of Hydromorphological Alterations on Mayfly Assemblages of a Mid-Sized Lowland River in South-Eastern Europe
Authors: Vilenica Marina , Vidaković Maoduš Iva , Mihaljević Zlatko
Published: May 2022

Historically, rivers have been anthropogenically modified for different purposes worldwide (e.g., flood control, drinking water abstraction, and land drainage). Although the majority of European lowland rivers are under the impact of multiple stressors (water quality, hydromorphological alterations, land-use), the consequences of these influences on aquatic macroinvertebrates, including mayflies, have not been sufficiently studied. Therefore, with the aim of providing additional data on the response of mayflies to anthropogenic disturbances in riverine habitats, we studied mayfly assemblages and their relationship to environmental factors along a mid-sized lowland river in Croatia. No significant differences in mayfly species richness and their functional traits were observed among the three habitat groups with different levels of hydromorphological alterations (near-natural, moderately altered, and severely altered habitats). This could be related to the river’s connection to the numerous tributaries, and the variety of available microhabitats along the studied system, despite the existing hydromorphological pressures. A stronger relationship was found between mayflies and the physico-chemical water characteristics and land-use. Water temperature, water velocity, oxygen content, and nutrient (e.g., nitrogen, phosphorous) concentrations related to agricultural and urban land-use were found to be the most important factors shaping mayfly assemblages in the studied lotic lowland system. These results can contribute to the planning of management and conservation activities for lowland rivers and their biota according to the requirements of the European Water Framework Directive.
Prone Position Facilitates Creation of Ulnar-Basilic Arteriovenous Fistula
Authors: Gołębiowski Tomasz , Jerzak Patryk , Letachowicz Krzysztof , Konieczny Andrzej , Kusztal Mariusz , Gołębiowski Maciej , Banasik Mirosław , Sznajder Katarzyna , Krajewska Magdalena
Published: May 2022

Background: The distal ulnar-basilic arteriovenous fistula (UBAVF) is a rarely used alternative type of vascular access for haemodialysis. The location of the vein on the back aspect of the forearm forces an extremely uncomfortable external rotation of the upper limb during surgery when the patient is in a supine position. Methods: We present a new approach towards creating UBAVF, which involves placing the patient in the prone position, thus eliminating the aforementioned inconvenience. The procedure was performed and described in a 46-year-old patient with chronic kidney disease (CKD) due to diabetic nephropathy. In the period from September 2021 to December 2021, we created an additional three UBAVFs with such modifications. Results: All fistulas were patent both immediately after the procedure and 2 weeks after surgery. Conclusions: The prone position may improve the comfort of both the operator and the patient during the procedure. On top of this, it may have a positive impact on the quality of the arteriovenous anastomosis.
Corneal Dendritic Cell Dynamics Are Associated with Clinical Factors in Type 1 Diabetes
Authors: Colorado Luisa H. , Beecher Lauren , Pritchard Nicola , Al Rashah Khaled , Dehghani Cirous , Russell Anthony , Malik Rayaz A. , Efron Nathan , Edwards Katie
Published: May 2022

Time-lapsed in vivo corneal confocal microscopy (IVCCM) has shown that corneal dendritic cells (DCs) migrate at approximately 1 µm/min in healthy humans. We have undertaken IVCCM of the whorl region to compare the density of rounded DCs, and DCs with (wDCs) and without (woDCs) dendrites and dynamics; trajectory (length travelled/time), displacement (distance from origin to endpoint/time) speeds and persistence ratio (displacement/trajectory) of woDCs in subjects with type 1 diabetes (T1D) (n = 20) and healthy controls (n = 10). Only the wDC density was higher (p = 0.02) in subjects with T1D compared to controls. There was no significant difference in cell dynamics between subjects with T1D and controls. woDC density correlated directly with HDL cholesterol (r = 0.59, p = 0.007) and inversely with triglycerides (r = −0.61, p = 0.005), whilst round-shaped cell density correlated inversely with HDL cholesterol (r = −0.54, p = 0.007). Displacement, trajectory, and persistency correlated significantly with eGFR (mL/min) (r = 0.74, p < 0.001; r = 0.48, p = 0.031; r = 0.58, p = 0.008, respectively). We show an increase in wDC density but no change in any other DC sub-type or alteration in cell dynamics in T1D. However, there were associations between DC density and lipid parameters and between DC dynamics and renal function. IVCCM provides evidence of a link between immune cell dynamics with lipid levels and renal function.
Antiepileptic Drugs and Their Dual Mechanism of Action on Carbonic Anhydrase
Authors: Magheru Calin , Magheru Sorina , Coltau Marcela , Hoza Anica , Moldovan Corina , Sachelarie Liliana , Gradinaru Irina , Hurjui Loredana Liliana , Marc Felicia , Farcas Dorina Maria
Published: May 2022

(1) Background: The benefit of using inhibitors of carbonic anhydrase (CA), such as acetazolamide, in the treatment of epilepsy has previously been described. (2) Methods: In this paper, the effect on CA of the most well-known antiepileptic drugs was studied in vitro and in vivo. The effects, after chronic treatment, of carbamazepine, phenytoin, valproate, primidone, clonazepam, and ethosuximide were studied in vitro on purified CA, isozyme I (CA I) and CA, and isozyme II (CA II) activity and in vivo on epileptic erythrocyte CA I and CA II activity. (3) Results: In vitro results showed that all antiepileptic drugs reduced purified CA II activity according to dose–response relationships and slightly inhibited CA I activity. In vivo results showed that the chronic administration of antiseizure drugs induced a progressive reduction in erythrocyte CA II activity in all the groups studied. This study shows that CA II inhibition can be induced both in vitro and in vivo by major antiepileptic agents as it might be one of the effective mechanisms of these anticonvulsant drugs. (4) Conclusions: The decrease in CA II activity in epileptic patients after antiseizure treatment suggests the involvement of CA II in the pathogenesis of epilepsy.
Clinical Relevance of Myopenia and Myosteatosis in Colorectal Cancer
Authors: Okugawa Yoshinaga , Kitajima Takahito , Yamamoto Akira , Shimura Tadanobu , Kawamura Mikio , Fujiwara Takumi , Mochiki Ikuyo , Okita Yoshiki , Tsujiura Masahiro , Yokoe Takeshi , Ohi Masaki , Toiyama Yuji
Published: May 2022

Sarcopenia was initially described as a decrease in muscle mass associated with aging and subsequently also as a consequence of underlying disease, including advanced malignancy. Accumulating evidence shows that sarcopenia has clinically significant effects in patients with malignancy, including an increased risk of adverse events associated with medical treatment, postoperative complications, and a poor survival outcome. Colorectal cancer (CRC) is one of the most common cancers worldwide, and several lines of evidence suggest that preoperative sarcopenia negatively impacts various outcomes in patients with CRC. In this review, we summarize the current evidence in this field and the clinical relevance of sarcopenia in patients with CRC from three standpoints, namely, the adverse effects of medical treatment, postoperative infectious complications, and oncological outcomes.
Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Zea mays L. Using Endophytic Aspergillus fumigatus
Authors: Abdelaziz Amer M. , El-Wakil Deiaa A. , Attia Mohamed S. , Ali Omar M. , AbdElgawad Hamada , Hashem Amr H.
Published: May 2022

Aspergillus flavus infection of vegetative tissues can affect the development and integrity of the plant and poses dangerous risks on human and animal health. Thus, safe and easily applied approaches are employed to inhibit A. flavus growth. To this end, the fungal endophyte, i.e., Aspergillus fumigatus, was used as a safe biocontrol agent to reduce the growth of A. flavus and its infection in maize seedlings. Interestingly, the safe endophytic A. fumigatus exhibited antifungal activity (e.g., 77% of growth inhibition) against A. flavus. It also reduced the creation of aflatoxins, particularly aflatoxin B1 (AFB1, 90.9%). At plant level, maize seedling growth, leaves and root anatomy and the changes in redox status were estimated. Infected seeds treated with A. fumigatus significantly improved the germination rate by 88.53%. The ultrastructure of the infected leaves showed severe disturbances in the internal structures, such as lack of differentiation in cells, cracking, and lysis in the cell wall and destruction in the nucleus semi-lysis of chloroplasts. Ultrastructure observations indicated that A. fumigatus treatment increased maize (leaf and root) cell wall thickness that consequentially reduced the invasion of the pathogenic A. flavus. It was also interesting that the infected seedlings recovered after being treated with A. fumigatus, as it was observed in growth characteristics and photosynthetic pigments. Moreover, infected maize plants showed increased oxidative stress (lipid peroxidation and H2O2), which was significantly mitigated by A. fumigatus treatment. This mitigation was at least partially explained by inducing the antioxidant defense system, i.e., increased phenols and proline levels (23.3 and 31.17%, respectively) and POD, PPO, SOD and CAT enzymes activity (29.50, 57.58, 32.14 and 29.52%, respectively). Overall, our study suggests that endophytic A. fumigatus treatment could be commercially used for the safe control of aflatoxins production and for inducing biotic stress tolerance of A. flavus-infected maize plants.
Diversity of Cantharellus (Cantharellales, Basidiomycota) in China with Description of Some New Species and New Records
Authors: Zhang Ming , Wang Chao-Qun , Gan Man-Shui , Li Yi , Shao Shi-Cheng , Qin Wei-Qiang , Deng Wang-Qiu , Li Tai-Hui
Published: May 2022

Cantharellus is a well-known genus of edible mushrooms, belonging to the family Hydnaceae in the class Agaricomycetes. In this study, a phylogenetic overview of Cantharellus subg. Cinnabarinus and C. subg. Parvocantharellus in China is carried out with the description of four new species. Species description are based on morphological characters of basidiomata and phylogenetic analyses of multi-locus dataset of 28S + tef1 + rpb2. Among the new species, two species, C. chrysanthus and C. sinocinnabarinus, belong to C. subg. Cinnabarinus and two new species, C. convexus and C. neopersicinus, belong to C. subg. Parvocantharellus. Species delimitation characters of the new taxa are compared with closely related species. In addition, three new records of Cantharellus are reported for China: C. albovenosus and C. citrinus of subg. Cinnabarinus and C. koreanus of subg. Parvocantharellus. A key to the species of subg. Cinnabarinus in China was provided.
Study on the Coupling System of Grain-Grass-Livestock of Herbivorous Animal Husbandry in Agricultural Areas: A Case Study of Najitun Farm of Hulunbuir Agricultural Reclamation in China
Authors: Han Chengji , Wang Guogang , Yang Hongbo
Published: May 2022

With the population growth and the upgrading of residents’ food consumption structures, the consumption demand for herbivorous animal products will maintain relatively rapid growth. However, restrictive factors for the development of herbivorous animal husbandry in pastoral areas have increased, and how to undertake herbivorous animal husbandry in agricultural areas has become the focus of widespread social concern. This study is based on survey data of Najitun Farm of Hulunbuir Agricultural Reclamation in China. Through field investigation and computer simulation technology of system dynamics, a development system of herbivorous animal husbandry in agricultural areas was established with the development of herbivorous animal husbandry at the core, and the balance of grassland-livestock and the combination of planting-breeding as the constraint. Moreover, the system designs the development strategy compared with the development of system inertia—strengthening and optimizing herbivorous animal husbandry and optimizing the structure of grain, economy, and the feed planting industry, and simulates the above three scenarios, respectively. The study found that without any development strategy, the inertia trend is subject to the influence of factors such as the scale of female livestock, epidemic diseases, and breeding level, so it is difficult to realize the sustainable development of the industry in the next five years. However, expanding the scale of breeding alone will occupy too much environmental capacity, and there will be a shortage in the supply of grass for a long time. According to the scheme of optimizing the structure of the grain, economy, and feed planting industry, it will not only provide feed sources for grass-feeding livestock of about 58,200 sheep units, but also realize the total agricultural output value of USD 7.02 million by the end of the 14th Five-Year Plan, which is 1.89 times of the inertia trend. At the same time, the nutrient demand of grass crops has alleviated 20.42% of the environmental pressure. Based on the results of this study, it is proven that herbivorous animal husbandry has a broad development potential in agricultural areas, and at the same time, it contributes to decisions of developing herbivorous animal husbandry in agricultural areas. This study has important theoretical and practical significance for expanding the industrial space and building a new type of planting-breeding relationship.
Bearing Fault Diagnosis via Incremental Learning Based on the Repeated Replay Using Memory Indexing (R-REMIND) Method
Authors: Zheng Junhui , Xiong Hui , Zhang Yuchang , Su Kaige , Hu Zheyuan
Published: May 2022

In recent years, deep-learning schemes have been widely and successfully used to diagnose bearing faults. However, as operating conditions change, the distribution of new data may differ from that of previously learned data. Training using only old data cannot guarantee good performance when handling new data, and vice versa. Here, we present an incremental learning scheme based on the Repeated Replay using Memory Indexing (R-REMIND) method for bearing fault diagnosis. R-REMIND can learn new information under various working conditions while retaining older information. First, we use a feature extraction network similar to the Inception-v4 neural network to collect bearing vibration data. Second, we encode the features by product quantization and store the features in indices. Finally, the parameters of the feature extraction and classification networks are updated using real and reconstructed features, and the model did not forget old information. The experiment results show that the R-REMIND model exhibits continuous learning ability with no catastrophic forgetting during sequential tasks.