bluuebunny/crossref_metadata_2025 huggingface dataset:
Invasion fronts and adaptive dynamics in a model for the growth of cell populations with heterogeneous mobility
Authors: LORENZI T. , PERTHAME B. , RUAN X.
Published: July 2021

We consider a model for the dynamics of growing cell populations with heterogeneous mobility and proliferation rate. The cell phenotypic state is described by a continuous structuring variable and the evolution of the local cell population density function (i.e. the cell phenotypic distribution at each spatial position) is governed by a non-local advection–reaction–diffusion equation. We report on the results of numerical simulations showing that, in the case where the cell mobility is bounded, compactly supported travelling fronts emerge. More mobile phenotypic variants occupy the front edge, whereas more proliferative phenotypic variants are selected at the back of the front. In order to explain such numerical results, we carry out formal asymptotic analysis of the model equation using a Hamilton–Jacobi approach. In summary, we show that the locally dominant phenotypic trait (i.e. the maximum point of the local cell population density function along the phenotypic dimension) satisfies a generalised Burgers’ equation with source term, we construct travelling-front solutions of such transport equation and characterise the corresponding minimal speed. Moreover, we show that, when the cell mobility is unbounded, front edge acceleration and formation of stretching fronts may occur. We briefly discuss the implications of our results in the context of glioma growth.
Pedagogical benefits of Chinese-American virtual exchange: A study of student perceptions
Authors: Luo Han , Yang Chunsheng
Published: July 2021

Despite the rapid development of virtual exchange in foreign language education, its use in the field of Chinese language education is still rather nascent. Adopting a mixed-methods research approach, this study focuses on examining the pedagogical benefits of a Chinese-American virtual exchange as mediated by students’ self-reported data. Two Chinese language classes (i.e. one at the elementary level and one at the intermediate level) at a small liberal arts college in the United States and a group of Chinese-speaking English majors at a Chinese university in Shanghai participated in the exchange. Analyses of four sources of qualitative data (i.e. WeChat group cultural discussion transcripts, Skype conversations, reflection journals, and end-of-program interviews) gave rise to four benefits of the exchange: promotion of cultural learning, improvement of Chinese language skills, enhancement of learning motivation, and establishment of a language learning community. Quantitative analyses of the end-of-semester questionnaire items show that the benefit regarding cultural learning, on average, received the highest rating, followed by community building and motivation enhancement, with improvement of Chinese skills being rated the lowest. Moreover, intermediate-level students evaluated all four benefits with higher ratings than elementary-level students. In particular, the two groups’ quantitative evaluations in terms of Chinese skills and motivation differed significantly. However, both groups enthusiastically endorsed the benefits of cultural learning and community building. The pedagogical implications of these results are also discussed.
Trans-catheter closure of large PDA in adult patients with Amplatzer device: case series
Authors: Khajali Zahra , Firouzi Ata , Ghaderian Homa , Aliramezany Maryam
Published: July 2021

Ductus arteriosus is a physiological structure if not closed after birth, may lead to many complications. Today, trans-catheter closure of patent ductus arteriosus with Occluder devices is the preferred method. Surgical ligation is used only in certain cases such as large symptomatic patent ductus arteriosus in very small infants and premature babies; unfavourable structure of the duct or economic considerations. In this article, we described haemodynamic and morphological characteristics of five patients with large patent ductus arteriosus which were occluded with Amplatzer device.

From 23 January, 2010 to 31 July, 2018, five patients referred to our clinic with large patent ductus arteriosus and pulmonary arterial hypertension for further evaluation. After assessing them with various diagnostic methods, we decided to close defect with ventricular septal defect Occluder device. Patients aged 21–44 years and one of them was male. Ductus closure was successfully done with ventricular septal defect Occluder device. Closure was successful for all of them but in one case, whose device was embolized to pulmonary artery after 24 hr and he underwent surgery.

Trans-catheter closure of large patent ductus arteriosus in adult patients with pulmonary hypertension is feasible. Despite the fact that complications may occur even with the most experienced hands, the ‘double disk’ Amplatzer ventricular septal defect muscular Occluder could be advantageous in this setting.
Assessing the utility of screening electrocardiograms in paediatric patients following COVID-19
Authors: Gartenberg Ari J. , White Travus J. , Dang Khoi , Shah Maully , Paridon Stephen M. , Elias Matthew D.
Published: July 2021

Objective:

To determine the utility of screening electrocardiograms after severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection among children in detecting myocarditis related to coronavirus disease 2019 (COVID-19).

Study Design:

A retrospective chart review was performed at a large paediatric academic institution to identify patients with prior SARS-CoV-2 infection who received a screening electrocardiogram by their primary care providers and were subsequently referred for outpatient cardiology consultation due to an abnormal electrocardiogram. The outcomes were the results from their cardiology evaluations, including testing and final diagnoses.

Results:

Among 46 patients, during their preceding COVID-19 illness, the majority had mild symptoms, 4 were asymptomatic, and 1 had moderate symptoms. The median length of time from positive SARS-CoV-2 test to screening electrocardiogram was 22 days, and many electrocardiogram findings that prompted cardiology consultation were normal variants in asymptomatic adolescent athletes. Patients underwent frequent additional testing at their cardiology appointments: repeat electrocardiogram (72%), echocardiogram (59%), Holter monitor (11%), exercise stress test (7%), and cardiac MRI (2%). Five patients were incidentally diagnosed with CHD or structural cardiac abnormalities, and three patients had conduction abnormalities (pre-mature atrial contractions, pre-mature ventricular contractions, borderline prolonged QTc), although potentially incidental to COVID-19. No patients were diagnosed with myocarditis or ventricular dysfunction.

Conclusion:

In a small cohort of children with prior COVID-19, who were primarily either asymptomatic or mildly symptomatic, subsequent screening electrocardiograms identified various potential abnormalities prompting cardiology consultation, but no patient was diagnosed with myocarditis. Larger multi-centre studies are necessary to confirm these results and to evaluate those with more severe disease.
Terrorist campaigns and the growth of the Muslim population: a reply
Authors: Egger Clara , Magni-Berton Raul
Published: July 2021

A recently published paper in this journal (Choi, 2021) establishes a statistical link between, on the one hand, Islamist terrorist campaigns – including terrorist attacks and online propaganda – and, on the other the growth of the Muslim population. The author explains this result by stating that successful campaigns lead some individuals to convert to Islam. In this commentary, we intend to reply to this article by focusing on the impact of terrorist attacks on religious conversion. We first show that Choi's results suffer from theoretical flaws – a failure to comprehensively unpack the link between violence and conversion – and methodological shortcomings – a focus on all terrorist groups over a period where Islamist attacks were rare. This leads us to replicate Choi's analysis by distinguishing Islamist and non-Islamist terror attacks on a more adequate timeframe. By doing so, we no longer find empirical support for the relationship between terror attacks and the growth of the Muslim population. However, our analyses suggest that such a hypothesis may hold but only in contexts where the level and intensity of political violence are high.
CONDITIONAL LOGIC IS COMPLETE FOR CONVEXITY IN THE PLANE
Authors: MARTI JOHANNES
Published: July 2021

We prove completeness of preferential conditional logic with respect to convexity over finite sets of points in the Euclidean plane. A conditional is defined to be true in a finite set of points if all extreme points of the set interpreting the antecedent satisfy the consequent. Equivalently, a conditional is true if the antecedent is contained in the convex hull of the points that satisfy both the antecedent and consequent. Our result is then that every consistent formula without nested conditionals is satisfiable in a model based on a finite set of points in the plane. The proof relies on a result by Richter and Rogers showing that every finite abstract convex geometry can be represented by convex polygons in the plane.
Associations of dietary B vitamins intakes with depression in adults
Authors: Wu Yanjun , Li Suyun , Wang Weijing , Zhang Dongfeng
Published: July 2021

Abstract: Background: The impact of the dietary B vitamins intakes on the development of depression has been scarcely investigated. Thus, this study aimed to examine the associations of dietary B vitamins intakes with the risk of depression in American adults. Methods: The data we used in this study were from the National Health and Nutrition Examination Survey (NHANES) 2007-2014. We used the Logistic regression models to analyze the associations of the dietary intakes of B vitamins with the risk of depression. Results: 17,732 individuals (8,623 males and 9,109 females) were enrolled in the study and they were all 18 or older. Compared to the lowest quartile of dietary intake, the ORs (95%CIs) of the highest quartile were 0.64 (0.50–0.82), 0.78 (0.62–0.97), 0.60 (0.47–0.78), 0.65 (0.50–0.84), and 0.71 (0.54–0.95) for vitamin B1, vitamin B2, niacin, vitamin B6, and vitamin B12, respectively. Compared to the people whose dietary intakes below the RDA in the model 2, those with intake meeting the RDA of vitamin B1 (OR: 0.68; 95%CI: 0.56–0.84), niacin (OR: 0.65; 95%CI: 0.51–0.81), B6 (OR: 0.65; 95%CI: 0.52–0.81), or B12 (OR: 0.65; 95%CI: 0.48–0.88) had a lower risk of depression, severally. We also found a nonlinear negative association between dietary vitamin B1, vitamin B2, niacin, vitamin B6, and vitamin B12 intakes and the risk of depression in the dose-response analyses, severally. Conclusions: Our results suggested that dietary vitamin B1, vitamin B2, niacin, vitamin B6, and vitamin B12 intakes may be inversely associated with the risk of depression.
Seismic Attenuation and S‐Velocity Structures in Beneath Central America Using 1‐D Full‐Waveform Inversion
Authors: Borgeaud Anselme F. E. , Deschamps Frédéric
Published: July 2021

We use one‐dimensional full‐waveform inversion to simultaneously infer the S‐velocity () and anelastic () structures of the lowermost 480 km of the mantle in 11 contiguous corridors located beneath Northern South America and Central America. Previous studies in this region have reported the presence of slabs at the core‐mantle boundary (CMB) and large variations in the depth of the discontinuity, suggesting local temperature variations. We use transverse component waveform data cut around the ScS phase, including the Scd phase due to the discontinuity, from 31 events beneath South America recorded at USArray stations. The minimum period used in this study is 8 s, allowing us to constrain the fine‐scale structure of the region and the depth of the discontinuity. We correct the data for the effect of amplitude focusing due to lateral velocity anomalies (i.e., out‐of‐plane focusing effect) in on the inferred structure using three‐dimensional waveform calculations. We show that, in the explored region, corrections for out‐of‐plane focusing effects are necessary to obtain models that have a reasonable interpretation in terms of temperature and fraction of post‐perovskite (pPv). Our final models show regions with high , high , and an elevated discontinuity, surrounding a low‐velocity corridor with lower and a depressed discontinuity that can be explained by local temperature variations. However, differences between temperature perturbations inferred from and suggest lateral variations in the fraction of pPv.
Spatial Persistence of Water Chemistry Patterns Across Flow Conditions in a Mesoscale Agricultural Catchment
Authors: Gu S. , Casquin A. , Dupas R. , Abbott B. W. , Petitjean P. , Durand P. , Gruau G.
Published: July 2021

Protecting water quality at catchment scales is complicated by the high spatiotemporal variability in water chemistry. Consequently, determining pollutant sources requires costly monitoring strategies to diagnose causes and guide management solutions. However, recent studies have shown that spatial patterns in water chemistry can be persistent at catchment scales, potentially allowing identification of pollution sources and sinks with just a few sampling campaigns. Here, we tested a new method to quantify spatial persistence (SP) of water chemistry patterns with data from synoptic samplings in 22 headwater subcatchments within a 375 km2 catchment in western France (March 2018 to July 2019). This new method to quantify SP reduces dependence on long‐term metrics such as flow‐weighted concentrations, which are usually uncertain or unavailable. We applied the method to 16 ecologically relevant water quality parameters, including soluble reactive phosphorus, nitrate, and dissolved organic carbon. The results showed an average SP of 0.68 among parameters during the study period. For most parameters, SP was higher during the high‐flow winter period but lower and more variable during the low‐flow summer period. We found that the SP ultimately depended on the ratio between the temporal and spatial coefficients of variation (variance explained: 70%) rather than the temporal synchrony among subcatchments (variance explained: 4%). These results demonstrate that in these temperate catchments, synoptic sampling during the high‐flow winter period allows efficient identification of source and sink subcatchments, while more frequent samplings are needed to characterize ecological conditions at low flow.
Possibility of Stabilizing the Greenland Ice Sheet
Authors: Wang Xiuquan , Fenech Adam , Farooque Aitazaz A.
Published: July 2021

Recent acceleration in the retreat of the Greenland ice sheet under a warming climate has caused unprecedented challenges and threats to coastal communities due to the rising sea level and increasing storm surges. This raises a critical question from a climate mitigation perspective: Would there still be a chance to stabilize the Greenland ice sheet if the carbon reduction goals of the Paris Agreement could be met? Here, we show that there is indeed a possibility for stabilizing the Greenland ice sheet with the low‐emission scenario of RCP2.6. In particular, RCP2.6 would potentially limit the warming in Greenland below 1°C within next 30 years and constrain its loss of ice sheet coverage below 10%. After 2050, the annual mean temperature in Greenland is likely to be stabilized and no further loss is expected to its ice sheet. However, the effective window for this chance will be closing after 2020. If no effective carbon reduction policies are being taken now, we are very likely to enter a continuous warming pathway and lose the chance of stabilizing the Greenland ice sheet.
Kronian Magnetospheric Reconnection Statistics Across Cassini’s Lifetime
Authors: Garton T. M. , Jackman C. M. , Smith A. W.
Published: July 2021

Magnetic reconnection is a fundamental physical process in planetary magnetospheres, in which plasma can be exchanged between the solar wind and a planetary magnetosphere, and material can be disconnected and ultimately lost from a magnetosphere. Magnetic reconnection in a planetary magnetotail can result in the release of plasmoids downtail and dipolarizations planetward of an x‐line. The signatures of these products include characteristic deflections in the north‐south component of the magnetic field which can be detected by in‐situ spacecraft. These signatures have been identified by eye, semi‐automated algorithms, and recently machine learning (ML) methods. Here, we apply statistical analysis to the most thorough catalog of Kronian magnetospheric reconnection signatures created through ML methods to improve understanding of magnetospheric evolution. This research concludes that no quasi‐steady position of the magnetotail x‐line exists within 70 . This research introduces prediction equations to estimate the distribution of duration of plasmoid passage over the spacecraft (, bin width = 1 min) and north‐south field deflection (, bin width = 0.25 nT) expected to be identified by an orbiting spacecraft across a year of observations. Furthermore, this research finds a local time asymmetry for reconnection identifications, with a preference for dusk‐side over dawn‐side. This may indicate a preference for Vasyliunas style reconnection over Dungey style for Saturn. Finally, through these distributions, the reconnection rate of Saturn’s magnetotail can be estimated as 3.22 reconnection events per day, with a resulting maximum mass loss from plasmoids of 44.87 kg on average, which is comparable with the magnetospheric mass loading from Enceladus (8–250 kg ).
An increase in VGF expression through a rapid, transcription-independent, autofeedback mechanism improves cognitive function
Authors: Lin Wei-Jye , Zhao Yan , Li Zhe , Zheng Shuyu , Zou Jin-lin , Warren Noël A. , Bali Purva , Wu Jingru , Xing Mengdan , Jiang Cheng , Tang Yamei , Salton Stephen R. , Ye Xiaojing
Published: July 2021

The release of neuropeptides from dense core vesicles (DCVs) modulates neuronal activity and plays a critical role in cognitive function and emotion. The granin family is considered a master regulator of DCV biogenesis and the release of DCV cargo molecules. The expression of the VGF protein (nonacronymic), a secreted neuropeptide precursor that also belongs to the extended granin family, has been previously shown to be induced in the brain by hippocampus-dependent learning, and its downregulation is mechanistically linked to neurodegenerative diseases such as Alzheimer’s disease and other mood disorders. Currently, whether changes in translational efficiency of Vgf and other granin mRNAs may be associated and regulated with learning associated neural activity remains largely unknown. Here, we show that either contextual fear memory training or the administration of TLQP-62, a peptide derived from the C-terminal region of the VGF precursor, acutely increases the translation of VGF and other granin proteins, such as CgB and Scg2, via an mTOR-dependent signaling pathway in the absence of measurable increases in mRNA expression. Luciferase-based reporter assays confirmed that the 3′-untranslated region (3′UTR) of the Vgf mRNA represses VGF translation. Consistently, the truncation of the endogenous Vgf mRNA 3′UTR results in substantial increases in VGF protein expression both in cultured primary neurons and in brain tissues from knock in mice expressing a 3′UTR-truncation mutant encoded by the modified Vgf gene. Importantly, Vgf 3′UTR-truncated mice exhibit enhanced memory performance and reduced anxiety- and depression-like behaviors. Our results therefore reveal a rapid, transcription-independent induction of VGF and other granin proteins after learning that are triggered by the VGF-derived peptide TLQP-62. Our findings suggest that the rapid, positive feedforward increase in the synthesis of granin family proteins might be a general mechanism to replenish DCV cargo molecules that have been released in response to neuronal activation and is crucial for memory function and mood stability.
A momentary assessment study on emotional and biological stress in adult males and females with autism spectrum disorder
Authors: van der Linden Kim , Simons Claudia , Viechtbauer Wolfgang , Ottenheijm Emmy , van Amelsvoort Thérèse , Marcelis Machteld
Published: July 2021

Prospective momentary psychological and biological measures of real-time daily life stress experiences have been examined in several psychiatric disorders, but not in adults with an autism spectrum disorder (ASD). The current electronic self-monitoring study examined associations between momentary daily life stressors and (i) negative affect (NA; emotional stress reactivity) and (ii) cortisol levels (biological stress reactivity) in males and females with ASD (N = 50) and without ASD (N = 51). The Experience Sampling Method, including saliva sampling, was used to measure three types of daily life stress (activity-related, event-related, and social stress), NA, and cortisol. Multilevel regression analyses demonstrated significant interactions between group and stress (i.e., activity-related and event-related stress) in the model of NA, indicating stronger emotional stress reactivity in the ASD than in the control group. In the model of cortisol, none of the group × stress interactions were significant. Male/female sex had no moderating effect on either emotional or biological stress reactivity. In conclusion, adults with ASD showed a stronger emotional stress (but not cortisol) reactivity in response to unpleasant daily life events and activities. The findings highlight the feasibility of electronic self-monitoring in individuals with ASD, which may contribute to the development of more personalized stress-management approaches.
Multi-level dilated residual network for biomedical image segmentation
Authors: Gudhe Naga Raju , Behravan Hamid , Sudah Mazen , Okuma Hidemi , Vanninen Ritva , Kosma Veli-Matti , Mannermaa Arto
Published: July 2021

We propose a novel multi-level dilated residual neural network, an extension of the classical U-Net architecture, for biomedical image segmentation. U-Net is the most popular deep neural architecture for biomedical image segmentation, however, despite being state-of-the-art, the model has a few limitations. In this study, we suggest replacing convolutional blocks of the classical U-Net with multi-level dilated residual blocks, resulting in enhanced learning capability. We also propose to incorporate a non-linear multi-level residual blocks into skip connections to reduce the semantic gap and to restore the information lost when concatenating features from encoder to decoder units. We evaluate the proposed approach on five publicly available biomedical datasets with different imaging modalities, including electron microscopy, magnetic resonance imaging, histopathology, and dermoscopy, each with its own segmentation challenges. The proposed approach consistently outperforms the classical U-Net by 2%, 3%, 6%, 8%, and 14% relative improvements in dice coefficient, respectively for magnetic resonance imaging, dermoscopy, histopathology, cell nuclei microscopy, and electron microscopy modalities. The visual assessments of the segmentation results further show that the proposed approach is robust against outliers and preserves better continuity in boundaries compared to the classical U-Net and its variant, MultiResUNet.
Exosomal hsa_circRNA_104484 and hsa_circRNA_104670 may serve as potential novel biomarkers and therapeutic targets for sepsis
Authors: Tian Chang , Liu Jiaying , Di Xin , Cong Shan , Zhao Min , Wang Ke
Published: July 2021

In order to explore the role of exosomal circRNAs in the occurrence and development of sepsis, we looked for potential diagnostic markers to accurately identify sepsis and to lay a molecular basis for precise treatment. Ultracentrifugation was used to extract exosomes from the serum of patients with sepsis and healthy individuals. Then, changes in circRNA expression in exosomes were studied by circRNA microarray analysis. Gene ontology (GO) analysis and Kyoto City Encyclopaedia of Genes and Genomes (KEGG) pathway analysis were used to annotate the biological functions and pathways of genes, and a circRNA-miRNA-mRNA regulatory network was constructed. In the microarray analysis, 132 circRNAs were significantly differentially expressed, including 80 and 52 that were upregulated and downregulated, respectively. RT-qPCR verified the results of microarray analysis: hsa_circRNA_104484 and hsa_circRNA_104670 were upregulated in sepsis serum exosomes. ROC analysis showed that hsa_circRNA_104484 and hsa_circRNA_104670 in serum exosomes have the potential to be used as diagnostic markers for sepsis. The circRNA-miRNA-mRNA network predicted the potential regulatory pathways of differentially expressed circRNAs. There are differences in the expression of circRNA in serum exosomes between patients with sepsis and healthy individuals, which may be involved in the occurrence and development of the disease. Among them, elevations in hsa_circRNA_104484 and hsa_circRNA_104670 could be used as novel diagnostic biomarkers and molecular therapeutic targets.
The value of autopsy in preterm infants at a Swedish tertiary neonatal intensive care unit 2002–2018
Authors: Hoffsten Alice , Markasz Laszlo , Ericson Katharina , Nelin Leif D. , Sindelar Richard
Published: July 2021

Reliable data on causes of death (COD) in preterm infants are needed to assess perinatal care and current clinical guidelines. In this retrospective observational analysis of all deceased preterm infants born < 37 weeks’ gestational age (n = 278) at a Swedish tertiary neonatal intensive care unit, we compared preliminary COD from Medical Death Certificates with autopsy defined COD (2002–2018), and assessed changes in COD between two periods (period 1:2002–2009 vs. period 2:2011–2018; 2010 excluded due to centralized care and seasonal variation in COD). Autopsy was performed in 73% of all cases and was more than twice as high compared to national infant autopsy rates (33%). Autopsy revised or confirmed a suspected preliminary COD in 34.9% of the cases (23.6% and 11.3%, respectively). Necrotizing enterocolitis (NEC) as COD increased between Period 1 and 2 (5% vs. 26%). The autopsy rate did not change between the two study periods (75% vs. 71%). We conclude that autopsy determined the final COD in a third of cases, while the incidence of NEC as COD increased markedly during the study period. Since there is a high risk to determine COD incorrectly based on clinical findings in preterm infants, autopsy remains a valuable method to obtain reliable COD.
Impact of antifungal stewardship interventions on the susceptibility of colonized Candida species in pediatric patients with malignancy
Authors: Amanati Ali , Badiee Parisa , Jafarian Hadis , Ghasemi Fatemeh , Nematolahi Samane , Haghpanah Sezaneh , Hamzavi Seyedeh Sedigheh
Published: July 2021

There is a worldwide concern regarding the antimicrobial resistance and the inappropriate use of antifungal agents, which had led to an ever-increasing antifungal resistance. This study aimed to identify the antifungal susceptibility of colonized Candida species isolated from pediatric patients with cancer and evaluate the clinical impact of antifungal stewardship (AFS) interventions on the antifungal susceptibility of colonized Candida species. Candida species colonization was evaluated among hospitalized children with cancer in a tertiary teaching hospital, Shiraz 2017–2018. Samples were collected from the mouth, nose, urine, and stool of the patients admitted to our center and cultured on sabouraud dextrose agar. The isolated yeasts identified by polymerase chain reaction–restriction fragment length polymorphisms (PCR–RFLP). DNA Extracted and PCR amplification was performed using the ITS1 and ITS4 primer pairs and Msp I enzyme. The broth microdilution method was used to determine the minimum inhibitory concentrations (MICs) for amphotericin B, caspofungin, and azoles. The prevalence of Candida albicans in the present study was significantly higher than other Candida species. Candida albicans species were completely susceptible to the azoles. The susceptibility rate of C. albicans to amphotericin B and caspofungin was 93.1% and 97.1%, respectively. The fluconazole MIC values of Candida albicans decreased significantly during the post-AFS period (P < 0.001; mean difference: 72.3; 95% CI of the difference: 47.36–98.62). We found that ‏52.5% (53/117) of the isolated C. albicans were azole-resistant before AFS implementation, while only 1.5% (2/102) of the isolates were resistant after implementation of the AFS program (P < 0.001). C. albicans fluconazole and caspofungin resistant rate also decreased significantly (P < 0.001) after implementation of the AFS program [26 (32.9%) versus 0 (0.0%) and 11 (10.9%) versus 1 (0.9%), respectively]. Besides, fluconazole use (p < 0.05) and fluconazole expenditure reduced significantly (about one thousand US$ per year) after the AFS program. Our results confirm the positive effect of optimized antifungal usage and bedside intervention on the susceptibility of Candida species after the implementation of the AFS program. C. albicans and C. glabrata exhibited a significant increase in susceptibility after the execution of the AFS program.
Dysregulation of anti-Mullerian hormone expression levels in mural granulosa cells of FMR1 premutation carriers
Authors: Friedman-Gohas Moran , Orvieto Raoul , Michaeli Abigael , Aizer Adva , Kirshenbaum Michal , Cohen Yoram
Published: July 2021

FMR1 premutation (55–200 CGG repeats) results in fragile X-associated primary ovarian insufficiency (FXPOI). We evaluated expression levels of folliculogenesis-related mediators, follicle-stimulating hormone (FSH) receptor and anti-Mullerian hormone (AMH), to gain insights into the mechanisms underlying the reduced ovarian function. Mural granulosa cells (MGCs) were collected from FMR1 premutation carriers and noncarriers undergoing IVF treatments. At baseline, MGCs of carriers demonstrated significantly higher mRNA expression levels of AMH (3.5 ± 2.2, n = 12 and 0.97 ± 0.5, n = 17, respectively; p = 0.0003) and FSH receptor (5.6 ± 2.8 and 2.7 ± 2.8, respectively; p = 0.02) and higher AMH protein expression on immunostaining. Accordingly, FMR1 premutation-transfected COV434 cells exhibited higher AMH protein expression than COV434 cells transfected with 20 CGG repeats. We conclude that FMR1 premutation may lead to dysregulation of AMH expression levels, probably due to a compensatory mechanism. Elucidating the pathophysiology of FXPOI may help in early detection of ovarian dysfunction and tailoring IVF treatments to FMR1 premutation carriers.
Heterodissemination: precision insecticide delivery to mosquito larval habitats by cohabiting vertebrates
Authors: Unlu Isik , Faraji Ary , Wang Yi , Rochlin Ilia , Gaugler Randy
Published: July 2021

Conventional larvicide delivery strategies originally developed for permanent and floodwater mosquitoes have proved suboptimal in the small, scattered, and cryptic larval habitats preferred by container-inhabiting Aedes mosquitoes. New methods such as autodissemination, wherein adult mosquitoes spread insecticides to their own larval habitats, have been under study. Another novel delivery method termed heterodissemination, i.e. larvicide delivery by other species sharing the same habitats, has also been proposed. We conducted a proof-of-concept study with four independent experiments using American bullfrogs (Lithobates catesbeianus) and green frogs Lithobates clamitans as carriers of pyriproxyfen, an insect growth regulator, under semi-field conditions in three different locations, two in New Jersey, and one in Utah. Frogs with attached slow-release pyriproxyfen tablets were introduced into outdoor enclosures with water containers. Water samples from the containers were periodically tested using larval Aedes albopictus and Culex pipiens mosquitoes to assess mortality and percent eclosure inhibition. Overall pupal mortality [95% credible intervals] estimated by Bayesian analysis for the treatment group was 73.4% [71.3–75.2] compared to 4.1% [2.9–5.5] for the control group. Mortality within treatment groups in four different experiments ranged from 41 to 100%, whereas control mortalities ranged from 0.5% to 11%. We conclude that heterodissemination is a promising and effective approach deserving of further study.
Metamaterial array based meander line planar antenna for cube satellite communication
Authors: Alam Touhidul , Almutairi Ali F. , Samsuzzaman Md , Cho Mengu , Islam Mohammad Tariqul
Published: July 2021

This research article presents a design and performance analysis of a metamaterial inspired ultra-high frequency (UHF) compact planar patch antenna for the CubeSat communication system that could be smoothly integrated with commercially available 2U Cube Satellite structure and onboard subsystem. The proposed antenna consists of two layers, one is two different width meander line antenna patch with partial ground plane and another layer is 3 × 2 near-zero-indexed metamaterial (NZIM) metamaterial array structure with ground plane. The NZIM array layer has been utilized to minimize the coupling effect with Cube Satellite structure and improve the frequency stability with enhanced antenna gain and efficiency. The fabricated antenna can operate within the lower UHF frequency band of 443.5–455 MHz. with an average peak gain of 2.5 dB. The designed antenna impedance stability characteristic has been explored after integration with the 2U Cube Satellite body layout. Besides, the antenna communication performance has been verified using 2U Cube Satellite free space path loss investigation. Small antenna volume with trade-off between the antenna size and performance are the key advantages of the proposed design, as the antenna occupies only 80 × 40 × 3.35 mm3 space of the 2U Cube Satellite body structure and the geometrical parameters can be designed to provide the best performance between 449 and 468.5 MHz.
Mapping habitat suitability for Asiatic black bear and red panda in Makalu Barun National Park of Nepal from Maxent and GARP models
Authors: Su Huiyi , Bista Manjit , Li Mingshi
Published: July 2021

Habitat evaluation is essential for managing wildlife populations and formulating conservation policies. With the rise of innovative powerful statistical techniques in partnership with Remote Sensing, GIS and GPS techniques, spatially explicit species distribution modeling (SDM) has rapidly grown in conservation biology. These models can help us to study habitat suitability at the scale of the species range, and are particularly useful for examining the overlapping habitat between sympatric species. Species presence points collected through field GPS observations, in conjunction with 13 different topographic, vegetation related, anthropogenic, and bioclimatic variables, as well as a land cover map with seven classification categories created by support vector machine (SVM) were used to implement Maxent and GARP ecological niche models. With the resulting ecological niche models, the suitable habitat for asiatic black bear (Ursus thibetanus) and red panda (Ailurus fulgens) in Nepal Makalu Barun National Park (MBNP) was predicted. All of the predictor variables were extracted from freely available remote sensing and publicly shared government data resources. The modeled results were validated by using an independent dataset. Analysis of the regularized training gain showed that the three most important environmental variables for habitat suitability were distance to settlement, elevation, and mean annual temperature. The habitat suitability modeling accuracy, characterized by the mean area under curve, was moderate for both species when GARP was used (0.791 for black bear and 0.786 for red panda), but was moderate for black bear (0.857), and high for red panda (0.920) when Maxent was used. The suitable habitat estimated by Maxent for black bear and red panda was 716 km2 and 343 km2 respectively, while the suitable area determined by GARP was 1074 km2 and 714 km2 respectively. Maxent predicted that the overlapping area was 83% of the red panda habitat and 40% of the black bear habitat, while GARP estimated 88% of the red panda habitat and 58% of the black bear habitat overlapped. The results of land cover exhibited that barren land covered the highest percentage of area in MBNP (36.0%) followed by forest (32.6%). Of the suitable habitat, both models indicated forest as the most preferred land cover for both species (63.7% for black bear and 61.6% for red panda from Maxent; 59.9% black bear and 58.8% for red panda from GARP). Maxent outperformed GARP in terms of habitat suitability modeling. The black bear showed higher habitat selectivity than red panda. We suggest that proper management should be given to the overlapping habitats in the buffer zone. For remote and inaccessible regions, the proposed methods are promising tools for wildlife management and conservation, deserving further popularization.
Cortical correlates in upright dynamic and static balance in the elderly
Authors: Rubega Maria , Formaggio Emanuela , Di Marco Roberto , Bertuccelli Margherita , Tortora Stefano , Menegatti Emanuele , Cattelan Manuela , Bonato Paolo , Masiero Stefano , Del Felice Alessandra
Published: July 2021

Falls are the second most frequent cause of injury in the elderly. Physiological processes associated with aging affect the elderly’s ability to respond to unexpected balance perturbations, leading to increased fall risk. Every year, approximately 30% of adults, 65 years and older, experiences at least one fall. Investigating the neurophysiological mechanisms underlying the control of static and dynamic balance in the elderly is an emerging research area. The study aimed to identify cortical and muscular correlates during static and dynamic balance tests in a cohort of young and old healthy adults. We recorded cortical and muscular activity in nine elderly and eight younger healthy participants during an upright stance task in static and dynamic (core board) conditions. To simulate real-life dual-task postural control conditions, the second set of experiments incorporated an oddball visual task. We observed higher electroencephalographic (EEG) delta rhythm over the anterior cortex in the elderly and more diffused fast rhythms (i.e., alpha, beta, gamma) in younger participants during the static balance tests. When adding a visual oddball, the elderly displayed an increase in theta activation over the sensorimotor and occipital cortices. During the dynamic balance tests, the elderly showed the recruitment of sensorimotor areas and increased muscle activity level, suggesting a preferential motor strategy for postural control. This strategy was even more prominent during the oddball task. Younger participants showed reduced cortical and muscular activity compared to the elderly, with the noteworthy difference of a preferential activation of occipital areas that increased during the oddball task. These results support the hypothesis that different strategies are used by the elderly compared to younger adults during postural tasks, particularly when postural and cognitive tasks are combined. The knowledge gained in this study could inform the development of age-specific rehabilitative and assistive interventions.
Bacillus firmus I-1582 promotes plant growth and impairs infection and development of the cyst nematode Heterodera schachtii over two generations
Authors: Huang Mengmeng , Bulut Aylin , Shrestha Bidhya , Matera Christiane , Grundler Florian M. W. , Schleker A. Sylvia S.
Published: July 2021

Plant-parasitic nematodes wreak havoc on crops by root parasitism worldwide. An approach to combat nematode root parasitism is the application of antagonistic microbes like the rhizobacterium Bacillus firmus I-1582 which is promoted as biological control agent. Although B. firmus is a known nematode antagonist in general, the underlying mechanisms about its interaction with nematodes and plants have not yet been elucidated. Therefore, we explored the influence of B. firmus I-1582 as well as its extracellular and secreted molecules on plant–nematode interaction utilizing the plant–pathogen system Arabidopsis thaliana–Heterodera schachtii. We demonstrated that B. firmus I-1582 is attracted by A. thaliana root exudates, particularly by those of young plants. The bacterium colonized the root and showed a strictly pH-dependent development and plant growth promotion effect. Our results revealed that root colonization by B. firmus I-1582 significantly protected A. thaliana from infestation by the beet cyst nematode whereas dead bacterial cells or the culture supernatant were not effective. The bacterium also negatively affected nematode reproduction as well as pathogenicity and development of next generation nematodes. The obtained results highlight B. firmus I-1582 as a promising biocontrol agent that is well suited as an element of integrated control management strategies in sustainable agriculture.
CPT to RVU conversion improves model performance in the prediction of surgical case length
Authors: Garside Nicholas , Zaribafzadeh Hamed , Henao Ricardo , Chung Royce , Buckland Daniel
Published: July 2021

Methods used to predict surgical case time often rely upon the current procedural terminology (CPT) code as a nominal variable to train machine-learned models, however this limits the ability of the model to incorporate new procedures and adds complexity as the number of unique procedures increases. The relative value unit (RVU, a consensus-derived billing indicator) can serve as a proxy for procedure workload and could replace the CPT code as a primary feature for models that predict surgical case length. Using 11,696 surgical cases from Duke University Health System electronic health records data, we compared boosted decision tree models that predict individual case length, changing the method by which the model coded procedure type; CPT, RVU, and CPT–RVU combined. Performance of each model was assessed by inference time, MAE, and RMSE compared to the actual case length on a test set. Models were compared to each other and to the manual scheduler method that currently exists. RMSE for the RVU model (60.8 min) was similar to the CPT model (61.9 min), both of which were lower than scheduler (90.2 min). 65.2% of our RVU model’s predictions (compared to 43.2% from the current human scheduler method) fell within 20% of actual case time. Using RVUs reduced model prediction time by ninefold and reduced the number of training features from 485 to 44. Replacing pre-operative CPT codes with RVUs maintains model performance while decreasing overall model complexity in the prediction of surgical case length.
mTOR inhibitors potentially reduce TGF-β2-induced fibrogenic changes in trabecular meshwork cells
Authors: Igarashi Nozomi , Honjo Megumi , Aihara Makoto
Published: July 2021

We examined the effects of mTOR inhibitors on the fibrotic response induced by transforming growth factor-beta2 (TGF-β2) in cultured human trabecular meshwork (hTM) cells. TGF-β2-induced expression of fibronectin, collagen type I, alpha 1 chain (COL1A1), and alpha-smooth muscle actin (αSMA) in hTM cells was examined in the presence or absence of mTOR inhibitors using quantitative real-time polymerase chain reaction, Western blotting, and immunohistochemistry. The migration rates of hTM cells were examined in the presence of TGF-β2 with or without mTOR inhibitors. An in vitro study showed that the expression of fibronectin, COL1A1, and αSMA was upregulated by TGF-β2 treatment of hTM cells; such upregulation was significantly suppressed by mTOR inhibitors. The inhibitors significantly reduced the migration rate of TGF-β2-stimulated hTM cells. mTOR inhibitors may usefully reduce the fibrotic response of hTM cells and we may have to explore if it is also effective in in vivo model.
TIFA protein expression is associated with pulmonary arterial hypertension
Authors: Chang Hao-Chih , Wei Tong-You Wade , Wu Pei-Yu , Tsai Ming-Daw , Yu Wen-Chung , Chen Chen-Huan , Sung Shih-Hsien
Published: July 2021

Tumor necrosis factor receptor-associated factor-interacting protein with a forkhead-associated domain (TIFA), a key regulator of inflammation, may be involved in the pathogenesis of pulmonary arterial hypertension (PAH). A total of 48 PAH patients (age 50.1 ± 13.1 years, 22.9% men), 25 hypertensive subjects, and 26 healthy controls were enrolled. TIFA protein expression in peripheral blood mononuclear cells (PBMCs) and plasma interleukin (IL)-1β and tumor necrosis factor (TNF)-α were measured. Pulmonary arterial hemodynamics were derived from right heart catheterization. PAH patients had the highest expression of TIFA, TNF-α, and IL-1β. TIFA protein expression was significantly associated with IL-1β (r = 0.94; P < 0.001), TNF-α (r = 0.93; P < 0.001), mean pulmonary artery pressure (r = 0.41; P = 0.006), and pulmonary vascular resistance (r = 0.41; P = 0.007). TIFA protein expression could independently predict the presence of PAH (odds ratio [95% confidence interval per-0.1 standard deviation]: 1.72 [1.37–2.16]; P < 0.001) and outperformed echocardiographic estimation. Ex vivo silencing of TIFA protein expression in PBMCs led to the suppression of the cellular expression of IL-1β and TNF-α. IL-1β and TNF-α mediated 80.4% and 56.6% of the causal relationship between TIFA and PAH, respectively, supporting the idea that TIFA protein is involved in the pathogenesis of PAH.
Normative data and standard operating procedures for static and dynamic retinal vessel analysis as biomarker for cardiovascular risk
Authors: Streese Lukas , Lona Giulia , Wagner Jonathan , Knaier Raphael , Burri Andri , Nève Gilles , Infanger Denis , Vilser Walthard , Schmidt-Trucksäss Arno , Hanssen Henner
Published: July 2021

Retinal vessel phenotype is predictive for cardiovascular outcome. This cross-sectional population-based study aimed to quantify normative data and standard operating procedures for static and dynamic retinal vessel analysis. We analysed central retinal arteriolar (CRAE) and venular (CRVE) diameter equivalents, as well as retinal endothelial function, measured by flicker light‐induced maximal arteriolar (aFID) and venular (vFID) dilatation. Measurements were performed in 277 healthy individuals aged 20 to 82 years of the COmPLETE study. The mean range from the youngest compared to the oldest decade was 196 ± 13 to 166 ± 17 µm for CRAE, 220 ± 15 to 199 ± 16 µm for CRVE, 3.74 ± 2.17 to 3.79 ± 2.43% for aFID and 4.64 ± 1.85 to 3.86 ± 1.56% for vFID. Lower CRAE [estimate (95% CI): − 0.52 (− 0.61 to − 0.43)], CRVE [− 0.33 (− 0.43 to − 0.24)] and vFID [− 0.01 (− 0.26 to − 0.00)], but not aFID, were significantly associated with older age. Interestingly, higher blood pressure was associated with narrower CRAE [− 0.82 (− 1.00 to − 0.63)] but higher aFID [0.05 (0.03 to 0.07)]. Likewise, narrower CRAE were associated with a higher predicted aFID [− 0.02 (− 0.37 to − 0.01)]. We recommend use of defined standardized operating procedures and cardiovascular risk stratification based on normative data to allow for clinical implementation of retinal vessel analysis in a personalized medicine approach.