bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparison of Landscape Metrics for Three Different Level Land Cover/Land Use Maps
Authors: Sertel Elif , Topaloğlu Raziye , Şallı Betül , Yay Algan Irmak , Aksu Gül
Published: October 2018

This research aims to investigate how different landscape metrics are affected by the enhancement of the thematic classes in land cover/land use (LC/LU) maps. For this aim, three different LC/LU maps based on three different levels of CORINE (Coordination of Information on The Environment) nomenclature were created for the selected study area using GEOBIA (Geographic Object Based Image Analysis) techniques. First, second and third level LC/LU maps of the study area have five, thirteen and twenty-seven hierarchical thematic classes, respectively. High-resolution Spot 7 images with 1.5 m spatial resolution were used as the main Earth Observation data to create LC/LU maps. Additional geospatial data from open sources (OpenStreetMap and Wikimapia) were also integrated to the classification in order to identify some of the 2nd and 3rd level LC/LU classes. Classification procedure was initially conducted for Level 3 classes in which we developed decision trees to be used in object-based classification. Afterwards, Level 3 classes were merged to create Level 2 LC/LU map and then Level 2 classes were merged to create the Level 1 LC/LU map according to CORINE nomenclature. The accuracy of Level 1, Level 2, Level 3 maps are calculated as; 93.50%, 89.00%, 85.50% respectively. At the last stage, several landscape metrics such as Number of Patch (NP), Edge Density (ED), Largest Patch Index (LPI), Euclidean Nearest Neighbor Distance (ENN), Splitting Index (SPLIT) and Aggregation Index (AI) metrics and others were calculated for different level LC/LU maps and landscape metrics values were compared to analyze the impact of changing thematic details on landscape metrics. Our results show that, increasing the thematic detail allows landscape characteristics to be defined more precisely and ensure comprehensive assessment of cause and effect relationships between classes.
Line-Constrained Shape Feature for Building Change Detection in VHR Remote Sensing Imagery
Authors: Liu Haifei , Yang Minhua , Chen Jie , Hou Jialiang , Deng Min
Published: October 2018

Buildings represent the most relevant features of human activity in urban regions, but their change detection using very-high-resolution (VHR) remote sensing imagery is still a major challenge. Effective representation of the building is the key point in building change detection. The linear feature can indirectly represent the structure and distribution of man-made objects. Thus, this study proposes a shape feature-based building change detection method. Specifically, a line-constrained shape (LCS) feature is developed to capture the shape characteristics of buildings. This feature improves the discriminability between buildings and other ground objects by integrating the pixel shape feature and line segments. The building candidate area (BCA) is created in accordance with the distribution of the line segments in two-phase images. The problem space is constrained in a high-likelihood region of buildings because of the BCA. Comparative experimental results demonstrate that the combination of the spectral feature and the developed LCS feature achieves the best performance in object-based building change detection in VHR imagery.
Exploring Protein–Protein Interaction in the Study of Hormone-Dependent Cancers
Authors: Miki Yasuhiro , Iwabuchi Erina , Ono Katsuhiko , Sasano Hironobu , Ito Kiyoshi
Published: October 2018

Estrogen receptors promote target gene transcription when they form a dimer, in which two identical (homodimer) or different (heterodimer) proteins are bound to each other. In hormone-dependent cancers, hormone receptor dimerization plays pivotal roles, not only in the pathogenesis or development of the tumors, but also in the development of therapeutic resistance. Protein–protein interactions (PPIs), including dimerization and complex formation, have been also well-known to be required for proteins to exert their functions. The methods which could detect PPIs are genetic engineering (i.e., resonance energy transfer) and/or antibody technology (i.e., co-immunoprecipitation) using cultured cells. In addition, visualization of the target proteins in tissues can be performed using antigen–antibody reactions, as in immunohistochemistry. Furthermore, development of microscopic techniques (i.e., electron microscopy and confocal laser microscopy) has made it possible to visualize intracellular and/or intranuclear organelles. We have recently reported the visualization of estrogen receptor dimers in breast cancer tissues by using the in situ proximity ligation assay (PLA). PLA was developed along the lines of antibody technology development, and this assay has made it possible to visualize PPIs in archival tissue specimens. Localization of PPI in organelles has also become possible using super-resolution microscopes exceeding the resolution limit of conventional microscopes. Therefore, in this review, we summarize the methodologies used for studying PPIs in both cells and tissues, and review the recently reported studies on PPIs of hormones.
Oxidative Stress and the Kidney in the Space Environment
Authors: Pavlakou Paraskevi , Dounousi Evangelia , Roumeliotis Stefanos , Eleftheriadis Theodoros , Liakopoulos Vassilios
Published: October 2018

In space, the special conditions of hypogravity and exposure to cosmic radiation have substantial differences compared to terrestrial circumstances, and a multidimensional impact on the human body and human organ functions. Cosmic radiation provokes cellular and gene damage, and the generation of reactive oxygen species (ROS), leading to a dysregulation in the oxidants–antioxidants balance, and to the inflammatory response. Other practical factors contributing to these dysregulations in space environment include increased bone resorption, impaired anabolic response, and even difficulties in detecting oxidative stress in blood and urine samples. Enhanced oxidative stress affects mitochondrial and endothelial functions, contributes to reduced natriuresis and the development of hypertension, and may play an additive role in the formation of kidney stones. Finally, the composition of urine protein excretion is significantly altered, depicting possible tubular dysfunction.
iTRAQ-Based Proteomic Analysis of Ogura-CMS Cabbage and Its Maintainer Line
Authors: Han Fengqing , Zhang Xiaoli , Yang Limei , Zhuang Mu , Zhang Yangyong , Li Zhansheng , Fang Zhiyuan , Lv Honghao
Published: October 2018

Ogura cytoplasmic male sterility (CMS) contributes considerably to hybrid seed production in Brassica crops. To detect the key protein species and pathways involved in Ogura-CMS, we analysed the proteome of the cabbage Ogura-CMS line CMS01-20 and its corresponding maintainer line F01-20 using the isobaric tags for the relative and absolute quantitation (iTRAQ) approach. In total, 162 differential abundance protein species (DAPs) were identified between the two lines, of which 92 were down-accumulated and 70 were up-accumulated in CMS01-20. For energy metabolism in the mitochondrion, eight DAPs involved in oxidative phosphorylation were down-accumulated in CMS01-20, whereas in the tricarboxylic acid (TCA) cycle, five DAPs were up-accumulated, which may compensate for the decreased respiration capacity and may be associated with the elevated O2 consumption rate in Ogura-CMS plants. Other key protein species and pathways involved in pollen wall assembly and programmed cell death (PCD) were also identified as being male-sterility related. Transcriptome profiling revealed 3247 differentially expressed genes between the CMS line and the fertile line. In a conjoint analysis of the proteome and transcriptome data, 30 and 9 protein species/genes showed the same and opposite accumulation patterns, respectively. Nine noteworthy genes involved in sporopollenin synthesis, callose wall degeneration, and oxidative phosphorylation were presumably associated with the processes leading to male sterility, and their expression levels were validated by qRT-PCR analysis. This study will improve our understanding of the protein species involved in pollen development and the molecular mechanisms underlying Ogura-CMS.
Evaluation for Synergistic Effects by Combinations of Photodynamic Therapy (PDT) with Temoporfin (mTHPC) and Pt(II) Complexes Carboplatin, Cisplatin or Oxaliplatin in a Set of Five Human Cancer Cell Lines
Authors: Lange Carsten , Bednarski Patrick J.
Published: October 2018

The platinum(II) complexes carboplatin (CBDCA), cisplatin (CDDP) and oxaliplatin (1-OHP) are used as anticancer drugs in a large number of tumour chemotherapy regimens. Many attempts have been made to combine Pt(II)-based chemotherapy with alternative treatment strategies. One such alternative anticancer approach is known as photodynamic therapy (PDT), where a non-toxic photosensitizer (PS) produces oxidative stress via the formation of reactive oxygen species (ROS) after local illumination of the affected tissue. A very promising PS is 5,10,15,20-tetra(m-hydroxyphenyl)chlorin (mTHPC, Temoporfin), which is approved for the treatment of head and neck cancer in Europe. In the present study, a combination of mTHPC-mediated PDT and either CBDCA, CDDP, or 1-OHP was applied to five human cancer cell lines from different tumour origins. Cytotoxicity was determined by the MTT assay and synergistic effects on cytotoxicity were evaluated by calculation of Combination Indices (CI). Synergy was identified in some of the combinations, for example, with 1-OHP in three of the tested cell lines but antagonism was also observed for a number of combinations in certain cell lines. In cases of synergy, elevated ROS levels were observed after combination but apoptosis induction was not necessarily increased compared to a treatment with a single compound. Cell cycle analysis revealed a formation of apoptotic subG1 populations and S phase as well as G2/M phase arrests after combination. In conclusion, pre-treatment with mTHPC-PDT has the potential to sensitize some types of tumour cells towards Pt(II) complexes, in particular 1-OHP but synergy is highly dependent on the type of cancer.
Cell-Based Therapies for Cardiac Regeneration: A Comprehensive Review of Past and Ongoing Strategies
Authors: Ghiroldi Andrea , Piccoli Marco , Cirillo Federica , Monasky Michelle M. , Ciconte Giuseppe , Pappone Carlo , Anastasia Luigi
Published: October 2018

Despite considerable improvements in the treatment of cardiovascular diseases, heart failure (HF) still represents one of the leading causes of death worldwide. Poor prognosis is mostly due to the limited regenerative capacity of the adult human heart, which ultimately leads to left ventricular dysfunction. As a consequence, heart transplantation is virtually the only alternative for many patients. Therefore, novel regenerative approaches are extremely needed, and several attempts have been performed to improve HF patients’ clinical conditions by promoting the replacement of the lost cardiomyocytes and by activating cardiac repair. In particular, cell-based therapies have been shown to possess a great potential for cardiac regeneration. Different cell types have been extensively tested in clinical trials, demonstrating consistent safety results. However, heterogeneous efficacy data have been reported, probably because precise end-points still need to be clearly defined. Moreover, the principal mechanism responsible for these beneficial effects seems to be the paracrine release of antiapoptotic and immunomodulatory molecules from the injected cells. This review covers past and state-of-the-art strategies in cell-based heart regeneration, highlighting the advantages, challenges, and limitations of each approach.
Identification of Arbuscular Mycorrhiza Fungi Responsive microRNAs and Their Regulatory Network in Maize
Authors: Xu Yunjian , Zhu Suwen , Liu Fang , Wang Wei , Wang Xuewen , Han Guomin , Cheng Beijiu
Published: October 2018

Maize can form symbiotic relationships with arbuscular mycorrhiza (AM) fungus to increase productivity and resistance, but the miRNAs in maize responsible for this process have not been discovered. In this study, 155 known and 28 novel miRNAs were identified by performing high-throughput sequencing of sRNA in maize roots colonized by AM fungi. Similar to the profiles in other AM-capable plants, a large proportion of identified maize miRNAs were 24 nt in length. Fourteen and two miRNAs were significantly down- and up-regulated in response to AM fungus Glomus intraradices inoculation, respectively, suggesting potential roles of these miRNAs in AM symbiosis. Interestingly, 12 of 14 significantly down-regulated known maize miRNAs belong to the miR399 family, which was previously reported to be involved in the interaction between Medicago truncatula and AM fungi. This result indicated that the miR399 family should regulate AM symbiosis conservatively across different plant lineages. Pathway and network analyses showed that the differentially expressed miRNAs might regulate lipid metabolism and phosphate starvation response in maize during the symbiosis process via their target genes. Several members of the miR399 family and the miR397 family should be involved in controlling the fatty acid metabolism and promoting lipid delivering from plants to AM fungi. To the best of our knowledge, this is the first report on miRNAs mediating fatty acids from plant to AM fungi. This study provides insight into the regulatory roles of miRNAs in the symbiosis between plants and AM fungi.
First Assessment of the Thryssa vitrirostris (Engraulidae) Beach Seine Fishery in Northeastern Mozambique
Authors: Manuessa Bonifácio , Morais Eurico , Cerveira Borges Teresa , Teodósio Maria Alexandra , Leitão Francisco
Published: October 2018

Monthly length-frequency data, from 2009 to 2014, was used to estimate the growth parameters, mortality, and spawning season, and to assess the exploitation status of Thryssa vitrirostris (Engraulidae) fisheries in Pebane. The von Bertalanffy asymptotic length (L∞) and growth rate (K) were 25.1 cm (standard length) and 0.41 per year (standard length), respectively. Two proxy recruitment peaks were found: the first peak of recruitment occurs from April to July, and the second recruitment peak from September to October. The total estimated mortality rates (Z), natural mortality (M), and fishing mortality (F) were Z = 1.31, M = 0.92, and F = 0.39. For the beach seine gear, the size at first catch of T. vitrirostris was Lc25 = 4.43 cm. The 50% retention size of the catch was Lc50 = 5.39 cm. The retention probability analyses revealed a large rate of juvenile fishing mortality (54.2%). The estimated exploitation rate (0.30) was below the maximum exploitation rate (0.48), and above the optimal sustainable exploitation rate (E50 = 0.28), evidencing a sustainable fishery. However, under such an exploitation regime, it is advised that a continuously monitoring-survey of T. vitrirostris is maintained. An increase in migration of fishermen has been recently recorded in Pebane, due to its rich fisheries, which can increase the fishing effort and the risk of overexploitation if management measures (such as mesh size increase) are not taken in advance.
Drug-Induced Interstitial Lung Disease: A Systematic Review
Authors: Skeoch Sarah , Weatherley Nicholas , Swift Andrew J. , Oldroyd Alexander , Johns Christopher , Hayton Conal , Giollo Alessandro , Wild James M. , Waterton John C. , Buch Maya , Linton Kim , Bruce Ian N. , Leonard Colm , Bianchi Stephen , Chaudhuri Nazia
Published: October 2018

Background: Drug-induced interstitial lung disease (DIILD) occurs as a result of numerous agents, but the risk often only becomes apparent after the marketing authorisation of such agents. Methods: In this PRISMA-compliant systematic review, we aimed to evaluate and synthesise the current literature on DIILD. Results: Following a quality assessment, 156 full-text papers describing more than 6000 DIILD cases were included in the review. However, the majority of the papers were of low or very low quality in relation to the review question (78%). Thus, it was not possible to perform a meta-analysis, and descriptive review was undertaken instead. DIILD incidence rates varied between 4.1 and 12.4 cases/million/year. DIILD accounted for 3–5% of prevalent ILD cases. Cancer drugs, followed by rheumatology drugs, amiodarone and antibiotics, were the most common causes of DIILD. The radiopathological phenotype of DIILD varied between and within agents, and no typical radiological pattern specific to DIILD was identified. Mortality rates of over 50% were reported in some studies. Severity at presentation was the most reliable predictor of mortality. Glucocorticoids (GCs) were commonly used to treat DIILD, but no prospective studies examined their effect on outcome. Conclusions: Overall high-quality evidence in DIILD is lacking, and the current review will inform larger prospective studies to investigate the diagnosis and management of DIILD.
Apparent Diffusion Coefficient Is a Novel Imaging Biomarker of Myopathic Changes in Liver Cirrhosis
Authors: Surov Alexey , Paul Lisa , Meyer Hans , Schob Stefan , Engelmann Cornelius , Wienke Andreas
Published: October 2018

Diffusion weighted imaging can provide information regarding tissue composition and can quantitatively characterize different pathological changes by means of apparent diffusion coefficient (ADC). The study comprised of 114 patients with liver cirrhosis—22 women and 92 men with a mean age of 56.5 ± 9.0 years. In all patients, the Model for End Stage-Liver Disease (MELD) score was calculated. Furthermore, 12 healthy persons (5 women, 7 men), mean age, 42.1 ± 16.2 years, were investigated as a control group. In all cases, magnetic resonance imaging of the liver/trunk was performed using different 3T scanners and diffusion weighted images were obtained with a multi-shot SE-EPI sequence. In all cases, polygonal regions of interest were manually drawn on the ADC maps along the contours of the iliopsoas and paravertebral muscles. The comparison of ADC values in groups was performed by Mann-Whitney-U tests. The association between ADC and MELD score was calculated by Spearman’s rank correlation coefficient. ADC values of the skeletal musculature were statistically much higher in comparison to those in the control group: 1.85 ± 0.46 × 10−3 mm2 s−1 vs. 1.23 ± 0.12 × 10−3 mm2 s−1, p = 0.001. ADC values showed statistically significant correlation with the MELD score (r = 0.473, p = 0.0001). Furthermore, ADC values differed between the subgroups with different values of the MELD score. ADC values correlated slightly with lactate dehydrogenase (LDH) (r = 0.381, p = 0.0001) and tended to correlate with C-reactive protein (CRP) (r = 0.171, p = 0.07) and alanine aminotransferase (ALAT) (r = −0.167, p = 0.076). ADC can reflect muscle changes in liver cirrhosis and shows statistically significant correlation with the MELD score. Therefore, ADC can be used as an imaging biomarker of myopathic changes in liver cirrhosis.
Higher Screening Aldosterone to Renin Ratio in Primary Aldosteronism Patients with Diabetes Mellitus
Authors: Chang Chia-Hui , Hu Ya-Hui , Huang Kuo-How , Lin Yen-Hung , Tsai Yao-Chou , Wu Che-Hsiung , Yang Shao-Yu , Chang Chin-Chen , Lu Ching-Chu , Wu Kwan-Dun , Wu Vin-Cent
Published: October 2018

Accumulated evidence has shown that low renin hypertension is common in patients with diabetic nephropathy. However, the performance of aldosterone to renin ratio (ARR) in primary aldosteronism (PA) patients with diabetes has not been well validated. Here, we report the performance of screening ARR in PA patients with diabetes. The study enrolled consecutive patients and they underwent ARR testing at screening. Then the diagnosis of PA was confirmed from the Taiwan Primary Aldosteronism Investigation registration dataset. Generalized additive model smoothing plot was used to validate the performance of screening ARR in PA patients with or without diabetes. During this study period, 844 PA patients were confirmed and 136 (16.0%) among them had diabetes. Other 816 patients were diagnosed with essential hypertension and used as the control group and 89 (10.9%) among them had diabetes. PA patients with diabetes were older and had a longer duration of hypertensive latency, higher systolic blood pressure and lower glomerular filtration rate than those PA patients without diabetes. The cut-off value of ARR in the generalized additive model predicting PA was 65 ng/dL per ng/mL/h in diabetic patients, while 45 ng/dL per ng/mL/h in non-diabetic patients. There was a considerable prevalence of diabetes among PA patients, which might be capable of interfering with the conventional screening test. The best cut-off value of ARR, more than 65 ng/dL per ng/mL/h in PA patients with diabetes, was higher than those without diabetes.
Reduced Cardiorespiratory Capacity in Children with Autism Spectrum Disorders
Authors: Bricout Véronique-Aurélie , Pace Marion , Dumortier Léa , Baillieul Flavie , Favre-Juvin Anne , Guinot Michel
Published: October 2018

Background—Children with autistic spectrum disorders (ASDs) are frequently hampered by motor impairment. It limits them from regularly practicing physical activities and results in a lower physical fitness even though low cardiorespiratory fitness is one of the most important predictors of all-cause mortality. This study aimed to investigate the cardiorespiratory fitness of boys with ASD compared to typically developed children. Methods—forty male children participated. Twenty were control children (CONT—10.0 ± 1.6 years) and 20 were ASD children (ASD—10.7 ± 1.2 years; intellectual quotient > 70). All participants completed an incremental exercise test on a treadmill. An evaluation of motor characteristics by three tests was conducted (muscular strength; explosive power; flexibility). Assessments of daily physical activity were obtained by questionnaires (PAQ-C) and by actigraphy. Results—in the ASD group, aerobic capacity values (VO2peak), effort duration and maximal speed were significantly lower compared to CONT (p < 0.05). Flexibility, explosive power and muscular strength were significantly lower in ASD compared to CONT (p < 0.05). Similarities between all children were observed for physical activity evaluation by actigraphy and with the PAQ-C. Conclusions—children with ASD had lower cardiorespiratory fitness than CONT despite similar physical activity levels. Our results suggested that the difference may be due to motor discrepancies.
In the Eye of the Deceiver: Analyzing Eye Movements as a Cue to Deception
Authors: Borza Diana , Itu Razvan , Danescu Radu
Published: October 2018

Deceit occurs in daily life and, even from an early age, children can successfully deceive their parents. Therefore, numerous book and psychological studies have been published to help people decipher the facial cues to deceit. In this study, we tackle the problem of deceit detection by analyzing eye movements: blinks, saccades and gaze direction. Recent psychological studies have shown that the non-visual saccadic eye movement rate is higher when people lie. We propose a fast and accurate framework for eye tracking and eye movement recognition and analysis. The proposed system tracks the position of the iris, as well as the eye corners (the outer shape of the eye). Next, in an offline analysis stage, the trajectory of these eye features is analyzed in order to recognize and measure various cues which can be used as an indicator of deception: the blink rate, the gaze direction and the saccadic eye movement rate. On the task of iris center localization, the method achieves within pupil localization in 91.47% of the cases. For blink localization, we obtained an accuracy of 99.3% on the difficult EyeBlink8 dataset. In addition, we proposed a novel metric, the normalized blink rate deviation to stop deceitful behavior based on blink rate. Using this metric and a simple decision stump, the deceitful answers from the Silesian Face database were recognized with an accuracy of 96.15%.
Hydrodynamic Zone of Influence Due to a Floating Structure in a Fjordal Estuary—Hood Canal Bridge Impact Assessment
Authors: Khangaonkar Tarang , Nugraha Adi , Wang Taiping
Published: October 2018

Floating structures such as barges and ships affect near-field hydrodynamics and create a zone of influence (ZOI). Extent of the ZOI is of particular interest due to potential obstruction to and impact on out-migrating juvenile fish. Here, we present an assessment of ZOI from Hood Canal (Floating) Bridge, located within the 110-km-long fjord-like Hood Canal sub-basin in the Salish Sea, Washington. A field data collection program allowed near-field validation of a three-dimensional hydrodynamic model of Hood Canal with the floating bridge section embedded. The results confirm that Hood Canal Bridge, with a draft of 4.6 m covering ~85% of the width of Hood Canal, obstructs the brackish outflow surface layer. This induces increased local mixing near the bridge, causes pooling of water (up-current) during ebb and flood, and results in shadow/sheltering of water (down-current). The change in ambient currents, salinity, and temperature is highest at the bridge location and reduces to background levels with distance from the bridge. The ZOI extends ~20 m below the surface and varies from 2–3 km for currents, from 2–4 km for salinity, and from 2–5 km for temperature before the deviations with the bridge drop to <10% relative to simulated background conditions without the bridge present.
Regional Scale Risk-Informed Land-Use Planning Using Probabilistic Coastline Recession Modelling and Economical Optimisation: East Coast of Sri Lanka
Authors: Dastgheib Ali , Jongejan Ruben , Wickramanayake Mangala , Ranasinghe Roshanka
Published: October 2018

One of the measures that has been implemented widely to adapt to the effect of climate change in coastal zones is the implementation of set-back lines. The traditional approach of determining set-back lines is likely to be conservative, and thus pose unnecessary constraints on coastal zone development and fully utilising the potential of these high-return areas. In this study, we apply a newly developed risk-informed approach to determine the coastal set-back line at regional scale in a poor data environment. This approach aims to find the economic optimum by balancing the (potential) economic gain from investing in coastal zones and the risk of coastal retreat due to sea level rise and storm erosion. This application focusses on the east coast of Sri Lanka, which is experiencing rapid economic growth on one hand and severe beach erosion on the other hand. This area of Sri Lanka is a highly data-poor environment, and the data is mostly available from global databases and very limited measurement campaigns. Probabilistic estimates of coastline retreat are obtained from the application of Probabilistic Coastline Recession (PCR) framework. Economic data, such as the discount rate, rate of return of investment, cost of damage, etc., are collated from existing estimates/reports for the area. The main outcome of this study is a series of maps indicating the economically optimal set-back line (EOSL) for the ~200-km-long coastal region. The EOSL is established for the year 2025 to provide a stable basis for land-use planning decisions over the next two decades or so. The EOSLs thus determined range between 12 m and 175 m from the coastline. Sensitivity analyses show that strong variations in key economic parameters such as the discount rate have a disproportionately small impact on the EOSL.
Characterizing the Evolution of the Container Traffic Share in the Mediterranean Sea Using Hierarchical Clustering
Authors: Grifoll Manel , Karlis Thanassis , Ortego M. I.
Published: October 2018

This research investigates the traffic share evolution of the container throughput in the Mediterranean ports from 2000 to 2015 considering hierarchical clustering and concentration indexes. Compositional Data analysis techniques are used to illustrate periods with similar traffic share composition. Two different regions (East and West) in the Mediterranean Sea (Med) are selected in the function of the long haul services. The standard concentration indexes (i.e., concentration ratio, Gini coefficient, and Normalized Herfindahl-Hirschman) reveal a gentle decreasing of the concentration with relevant fluctuations mainly in the East region. This is due to the investment in port infrastructure in the area resulting from privatization initiatives in many Eastern Mediterranean countries. The periods obtained from the hierarchical clustering show a differentiated pattern in traffic share composition. For these periods, the shift-share results are consistent with traffic fluctuations and in line with the evolution of the concentration indexes. The combination of methods has allowed a good interpretation of the spatial and temporal evolution of the Med ports’ traffic being the methodology applicable elsewhere in the context of port system analysis.
Landscape Assessment and Economic Accounting in Wind Farm Programming: Two Cases in Sicily
Authors: Giuffrida Salvatore , Gagliano Filippo , Nocera Francesco , Trovato Maria Rosa
Published: October 2018

In recent years, the scientific interest for the economic and landscape impact of wind farms has increased. This paper presents a useful GIS tool that allows for helping policymakers and investors to identify promising areas for wind power generation as well as landscape impact and financial and economic sustainability of wind farms. The results of the research carried out for exploring the potential for wind energy in two territorial contexts of Sicily region are presented with the particular look at the possibilities of economic developing, stakeholders’ opportunities and obstacles in the policy, legal, and regulatory framework.
Cropland Abandonment in South African Smallholder Communal Lands: Land Cover Change (1950–2010) and Farmer Perceptions of Contributing Factors
Authors: Blair Dale , Shackleton Charlie M. , Mograbi Penelope J.
Published: October 2018

Despite agricultural land abandonment threatening the food security and the livelihoods of smallholder farmers, it is pervasive globally and in developing countries. Yet land abandonment is an understudied aspect of land use change in social–ecological systems. Here we provide more information on this phenomenon by exploring cropland abandonment during 1950–2010 in four former South African ‘homelands’—part of the ‘Apartheid’ era racially-based land allocation programs—characterized by rural, smallholder farmers. Cropland abandonment 1950–2010 was widespread in all surveyed sites (KwaZulu: 0.08% year−1, Transkei: 0.13% year−1, Lebowa: 0.23% year−1, Venda: 0.28% year−1), with rates peaking between 1970 and 1990, with concomitant increases (up to 0.16% year−1) of woody vegetation cover at the expense of grassland cover. Active and past farmers attributed cropland abandonment to a lack of draught power, rainfall variability and droughts, and a more modernized youth disinclined to living a marginal agrarian lifestyle. We discuss the potential social and ecological implications of abandoned croplands at the local and regional scales, as the deagrarianization trend is unlikely to abate considering the failure of current South African national agricultural incentives.
Two-Scale Tomography Based Finite Element Modeling of Plasticity and Damage in Aluminum Foams
Authors: Amani Yasin , Dancette Sylvain , Maire Eric , Adrien Jérôme , Lachambre Joël
Published: October 2018

In this study, finite element (FE) modeling of open-cell aluminum foams in tension was performed based on laboratory X-ray tomography scans of the materials at two different scales. High-resolution stitching tomography of the initial state allowed local intermetallic particles to be distinguished from internal defects in the solid phase of the foam. Lower-resolution scans were used to monitor the deformation and fracture in situ during loading. 3D image-based FE models of the foams were built to simulate the tensile behavior using a new microstructure-informed Gurson–Tvergaard–Needleman model. The new model allows quantitative consideration of the local presence of brittle intermetallic particles in the prediction of damage. It performs well in the discrimination of potential fracture zones in the foam, and can be easily adapted to any type of architectured material where both the global architecture and local microstructural details should be taken into account in the prediction of damage behavior.
Low-Temperature CVD Graphene Nanostructures on Cu and Their Corrosion Properties
Authors: Huang Wei-Hao , Lin Cheng-Hsuan , Lin Ben-Son , Sun Chia-Liang
Published: October 2018

Chemical vapor deposition (CVD) graphene is reported to effectively prevent the penetration of outer factors and insulate the underneath metals, hence achieving an anticorrosion purpose. However, there is little knowledge about their characteristics and corresponding corrosion properties, especially for those prepared under different parameters at low temperatures. Using electron cyclotron resonance chemical vapor deposition (ECR-CVD), we can successfully prepare graphene nanostructures on copper (Cu) at temperatures lower than 600 °C. Scanning electron microscopy (SEM), X-ray diffraction (XRD), Raman spectroscopy, and potentiodynamic polarization measurements were used to characterize these samples. In simulated seawater, i.e., 3.5 wt.% sodium chloride (NaCl) solution, the corrosion current density of one graphene-coated Cu fabricated at 400 °C can be 1.16 × 10−5 A/cm2, which is one order of magnitude lower than that of pure Cu. Moreover, the existence of tall graphene nanowalls was found not to be beneficial to the protection as a consequence of their layered orientation. These correlations among the morphology, structure, and corrosion properties of graphene nanostructures were investigated in this study. Therefore, the enhanced corrosion resistance in selected cases suggests that the low-temperature CVD graphene under appropriate conditions would be able to protect metal substrates against corrosion.
The Diffusion of Low-Energy Methyl Group on ITO Film Surface and Its Impact on Optical-Electrical Properties
Authors: Zhao Shiping , Lv Zhixuan , Guo Xuelin , Liu Chaoqian , Wang Hualin , Jiang Weiwei , Liu Shimin , Wang Nan , Cui Yunxian , Ding Wanyu , Han Bing , Ju Dongying
Published: October 2018

Indium tin oxide (ITO) film is one of the ideal candidates for transparent conductive cathode in methylammonium lead halide perovskite solar cells. Thus, the diffusion of methyl group in ITO film is inevitable, which could deteriorate the optical-electrical property of ITO film. In this study, ITO films with and without (100) preferred orientation were bombarded by the low-energy methyl group beam. After the bombardment, the optical-electrical property of ITO film without (100) preferred orientation deteriorated. The bombardment of methyl group had little influence on the optical-electrical property of ITO film with (100) preferred orientation. Finally, combining the crystallographic texture and chemical bond structure analysis, the diffusion mechanism of low-energy methyl group on ITO lattice and grain boundary, as well as the relation between the optical-electrical property and the diffusion of the methyl group, were discussed systematically. With the above results, ITO film with (100) preferred orientation could be an ideal candidate for transparent conductive cathode in methylammonium lead halide perovskite solar cells.
Ultrasonic Processing of Aluminum–Magnesium Alloys
Authors: Mills Kurt , Wang Gui , StJohn David , Dargusch Matthew
Published: October 2018

This study evaluated the effect of UltraSonic Treatment (UST) on a range of Al–Mg alloys. Previous research was carried out on single magnesium compositions. However, the amount and type of the alloy addition are known to affect the grain size even under UST, and the aim of this study was to determine whether or not alloy composition plays a similar role in the case of Al–Mg alloys. By testing binary Al–Mg alloys cast under regular casting conditions and under the presence of an ultrasonic field, it was found that while the addition of Mg solute is important, the amount of solute has little effect when UST is applied. It was observed that the grain size was barely affected by extra solute additions in this condition. This is due to the application of UST during solidification, which resulted in a dramatic reduction in the size of the nucleation free zone thus promoting many more successful nucleation events. Acoustic streaming is proposed as the main cause of this reduction in grain size.
High-Order Finite-Element Framework for the Efficient Simulation of Multifluid Flows
Authors: Metivet Thibaut , Chabannes Vincent , Ismail Mourad , Prud’homme Christophe
Published: October 2018

In this paper, we present a comprehensive framework for the simulation of Multifluid flows based on the implicit level-set representation of interfaces and on an efficient solving strategy of the Navier-Stokes equations. The mathematical framework relies on a modular coupling approach between the level-set advection and the fluid equations. The space discretization is performed with possibly high-order stable finite elements while the time discretization features implicit Backward Differentation Formulae of arbitrary order. This framework has been implemented within the Feel++ library, and features seamless distributed parallelism with fast assembly procedures for the algebraic systems and efficient preconditioning strategies for their resolution. We also present simulation results for a three-dimensional Multifluid benchmark, and highlight the importance of using high-order finite elements for the level-set discretization for problems involving the geometry of the interfaces, such as the curvature or its derivatives.
New Mammalian Target of Rapamycin (mTOR) Modulators Derived from Natural Product Databases and Marine Extracts by Using Molecular Docking Techniques
Authors: Ruiz-Torres Verónica , Losada-Echeberría Maria , Herranz-López Maria , Barrajón-Catalán Enrique , Galiano Vicente , Micol Vicente , Encinar José Antonio
Published: October 2018

Mammalian target of rapamycin (mTOR) is a PI3K-related serine/threonine protein kinase that functions as a master regulator of cellular growth and metabolism, in response to nutrient and hormonal stimuli. mTOR functions in two distinct complexes—mTORC1 is sensitive to rapamycin, while, mTORC2 is insensitive to this drug. Deregulation of mTOR’s enzymatic activity has roles in cancer, obesity, and aging. Rapamycin and its chemical derivatives are the only drugs that inhibit the hyperactivity of mTOR, but numerous side effects have been described due to its therapeutic use. The purpose of this study was to identify new compounds of natural origin that can lead to drugs with fewer side effects. We have used computational techniques (molecular docking and calculated ADMET (Absorption, Distribution, Metabolism, Excretion, and Toxicity) parameters) that have enabled the selection of candidate compounds, derived from marine natural products, SuperNatural II, and ZINC natural products, for inhibitors targeting, both, the ATP and the rapamycin binding sites of mTOR. We have shown experimental evidence of the inhibitory activity of eleven selected compounds against mTOR. We have also discovered the inhibitory activity of a new marine extract against this enzyme. The results have been discussed concerning the necessity to identify new molecules for therapeutic use, especially against aging, and with fewer side effects.
Kororamides, Convolutamines, and Indole Derivatives as Possible Tau and Dual-Specificity Kinase Inhibitors for Alzheimer’s Disease: A Computational Study
Authors: Llorach-Pares Laura , Nonell-Canals Alfons , Avila Conxita , Sanchez-Martinez Melchor
Published: October 2018

Alzheimer’s disease (AD) is becoming one of the most disturbing health and socioeconomic problems nowadays, as it is a neurodegenerative pathology with no treatment, which is expected to grow further due to population ageing. Actual treatments for AD produce only a modest amelioration of symptoms, although there is a constant ongoing research of new therapeutic strategies oriented to improve the amelioration of the symptoms, and even to completely cure the disease. A principal feature of AD is the presence of neurofibrillary tangles (NFT) induced by the aberrant phosphorylation of the microtubule-associated protein tau in the brains of affected individuals. Glycogen synthetase kinase-3 beta (GSK3β), casein kinase 1 delta (CK1δ), dual-specificity tyrosine phosphorylation regulated kinase 1A (DYRK1A) and dual-specificity kinase cdc2-like kinase 1 (CLK1) have been identified as the principal proteins involved in this process. Due to this, the inhibition of these kinases has been proposed as a plausible therapeutic strategy to fight AD. In this study, we tested in silico the inhibitory activity of different marine natural compounds, as well as newly-designed molecules from some of them, over the mentioned protein kinases, finding some new possible inhibitors with potential therapeutic application.
Study on Hot Press Forming Process of Large Curvilinear Generatrix Workpiece of Ti55 High-Temperature Titanium Alloy
Authors: Wu Fengyong , Xu Wenchen , Yang Zhongze , Guo Bin , Shan Debin
Published: October 2018

In order to manufacture complex curvilinear generatrix workpieces of high-temperature titanium alloy, the hot tensile behavior of Ti55 alloy sheet was tested and the hot press forming process was investigated using Finite Element Method (FEM) simulation and experiment. The hot tensile experiments of Ti55 rolled sheet were conducted at the temperatures of 800–900 °C with the strain rates of 0.001–0.1 s−1. According to the results of hot tensile tests and microstructure evolution, the proper hot press forming parameters were determined as the temperature of 850 °C and the strain rates of 0.001–0.01 s−1. The wrinkling mechanism in the transition region was analyzed and the initial blank sheet geometry was optimized by FE simulation of hot press forming. The two-step hot press forming process was better to produce the complex sheet workpiece of Ti55 alloy than the one-step hot forming scheme, which could restrain the wrinkling trend and ensure the microstructure and mechanical properties of the hot formed workpieces.
Surface Morphology and Its Influence on Cyclic Deformation Behavior of High-Mn TWIP Steel
Authors: Klein Matthias W. , Smaga Marek , Beck Tilmann
Published: October 2018

In this study, the dependence of the cyclic deformation behavior on the surface morphology of metastable austenitic HSD® 600 TWinning Induced Plasticity (TWIP) steel was investigated. This steel—with the alloying concept Mn-Al-Si—shows a fully austenitic microstructure with deformation-induced twinning at ambient temperature. Four different surface morphologies were analyzed: as-received with a so-called rolling skin, after up milling, after down milling, and a reference morphology achieved by polishing. The morphologies were characterized by X-Ray Diffraction (XRD), Focused Ion Beam (FIB), Scanning Electron Microscopy (SEM) as well as confocal microscopy methods and show significant differences in initial residual stresses, phase fractions, topographies and microstructures. For specimens with all variants of the morphologies, fatigue tests were performed in the Low Cycle Fatigue (LCF) and High Cycle Fatigue (HCF) regime to characterize the cyclic deformation behavior and fatigue life. Moreover, this study focused on the frequency-dependent self-heating of the specimens caused by cyclic plasticity in the HCF regime. The results show that both surface morphology and specimen temperature have a significant influence on the cyclic deformation behavior of HSD® 600 TWIP steel in the HCF regime.
Intracellular Fate of Universally Labelled 13C Isotopic Tracers of Glucose and Xylose in Central Metabolic Pathways of Xanthomonas oryzae
Authors: Shree Manu , K. Masakapalli Shyam
Published: October 2018

The goal of this study is to map the metabolic pathways of poorly understood bacterial phytopathogen, Xanthomonas oryzae (Xoo) BXO43 fed with plant mimicking media XOM2 containing glutamate, methionine and either 40% [13C5] xylose or 40% [13C6] glucose. The metabolic networks mapped using the KEGG mapper and the mass isotopomer fragments of proteinogenic amino acids derived from GC-MS provided insights into the activities of Xoo central metabolic pathways. The average 13C in histidine, aspartate and other amino acids confirmed the activities of PPP, the TCA cycle and amino acid biosynthetic routes, respectively. The similar labelling patterns of amino acids (His, Ala, Ser, Val and Gly) from glucose and xylose feeding experiments suggests that PPP would be the main metabolic route in Xoo. Owing to the lack of annotated gene phosphoglucoisomerase in BXO43, the 13C incorporation in alanine could not be attributed to the competing pathways and hence warrants additional positional labelling experiments. The negligible presence of 13C incorporation in methionine brings into question its potential role in metabolism and pathogenicity. The extent of the average 13C labelling in several amino acids highlighted the contribution of pre-existing pools that need to be accounted for in 13C-flux analysis studies. This study provided the first qualitative insights into central carbon metabolic pathway activities in Xoo.
Rational Design and Biotechnological Production of Novel AfpB-PAF26 Chimeric Antifungal Proteins
Authors: Heredero Marcos , Garrigues Sandra , Gandía Mónica , Marcos Jose F. , Manzanares Paloma
Published: October 2018

Antimicrobial peptides (AMPs) have been proposed as candidates to develop new antimicrobial compounds for medicine, agriculture, and food preservation. PAF26 is a synthetic antifungal hexapeptide obtained from combinatorial approaches with potent fungicidal activity against filamentous fungi. Other interesting AMPs are the antifungal proteins (AFPs) of fungal origin, which are basic cysteine-rich and small proteins that can be biotechnologically produced in high amounts. A promising AFP is the AfpB identified in the phytopathogen Penicillium digitatum. In this work, we aimed to rationally design, biotechnologically produce and test AfpB::PAF26 chimeric proteins to obtain designed AFPs (dAfpBs) with improved properties. The dAfpB6 and dAfpB9 chimeras could be produced using P. digitatum as biofactory and a previously described Penicillium chrysogenum-based expression cassette, but only dAfpB9 could be purified and characterized. Protein dAfpB9 showed subtle and fungus-dependent differences of fungistatic activity against filamentous fungi compared to native AfpB. Significantly, dAfpB9 lost the fungicidal activity of PAF26 and AfpB, thus disconnecting this activity from the fungistatic activity and mapping fungicidal determinants to the exposed loop L3 of AfpB, wherein modifications are located. This study provides information on the design and development of novel chimeric AFPs.