bluuebunny/crossref_metadata_2025 huggingface dataset:
Acute Neurological Involvement after Donor Lymphocyte Infusion for Post-Transplant Viral Infection: The Same Pattern of Novel Cancer Immunotherapy-Related CNS Toxicity?
Authors: Marcuzzi Annalisa , Rimondi Erika , Melloni Elisabetta , Zennaro Floriana , Sonzogni Aurelio , Leo Sara , Maximova Natalia
Published: March 2022

Early post-transplant is the critical phase for the success of hematopoietic stem cell transplantation (HSCT). New viral infections and the reactivations associated with complete ablation of the recipient’s T-cell immunity and inefficient reconstitution of the donor-derived system represent the main risks of HSCT. To date, the pharmacological treatments for post-HSCT viral infection-related complications have many limitations. Adoptive cell therapy (ACT) represents a new pharmacological strategy, allowing us to reconstitute the immune response to infectious agents in the post-HSC period. To demonstrate the potential advantage of this novel immunotherapy strategy, we report three cases of pediatric patients and the respective central nervous system complications after donor lymphocyte infusion.
Effect of Metformin on T2D-Induced MAM Ca2+ Uncoupling and Contractile Dysfunction in an Early Mouse Model of Diabetic HFpEF
Authors: Dia Maya , Leon Christelle , Chanon Stephanie , Bendridi Nadia , Gomez Ludovic , Rieusset Jennifer , Thibault Helene , Paillard Melanie
Published: March 2022

Diabetic cardiomyopathy (DCM) is a leading complication in type 2 diabetes patients. Recently, we have shown that the reticulum-mitochondria Ca2+ uncoupling is an early and reversible trigger of the cardiac dysfunction in a diet-induced mouse model of DCM. Metformin is a first-line antidiabetic drug with recognized cardioprotective effect in myocardial infarction. Whether metformin could prevent the progression of DCM remains not well understood. We therefore investigated the effect of a chronic 6-week metformin treatment on the reticulum-mitochondria Ca2+ coupling and the cardiac function in our high-fat high-sucrose diet (HFHSD) mouse model of DCM. Although metformin rescued the glycemic regulation in the HFHSD mice, it did not preserve the reticulum-mitochondria Ca2+ coupling either structurally or functionally. Metformin also did not prevent the progression towards cardiac dysfunction, i.e., cardiac hypertrophy and strain dysfunction. In summary, despite its cardioprotective role, metformin is not sufficient to delay the progression to early DCM.
Specialized Pro-Resolving Lipid Mediators: New Therapeutic Approaches for Vascular Remodeling
Authors: Díaz del Campo Lucía Serrano , Rodrigues-Díez Raquel , Salaices Mercedes , Briones Ana M. , García-Redondo Ana B.
Published: March 2022

Vascular remodeling is a typical feature of vascular diseases, such as atherosclerosis, aneurysms or restenosis. Excessive inflammation is a key mechanism underlying vascular remodeling via the modulation of vascular fibrosis, phenotype and function. Recent evidence suggests that not only augmented inflammation but unresolved inflammation might also contribute to different aspects of vascular diseases. Resolution of inflammation is mediated by a family of specialized pro-resolving mediators (SPMs) that limit immune cell infiltration and initiate tissue repair mechanisms. SPMs (lipoxins, resolvins, protectins, maresins) are generated from essential polyunsaturated fatty acids. Synthases and receptors for SPMs were initially described in immune cells, but they are also present in endothelial cells (ECs) and vascular smooth muscle cells (VSMCs), where they regulate processes important for vascular physiology, such as EC activation and VSMC phenotype. Evidence from genetic models targeting SPM pathways and pharmacological supplementation with SPMs have demonstrated that these mediators may play a protective role against the development of vascular remodeling in atherosclerosis, aneurysms and restenosis. This review focuses on the latest advances in understanding the role of SPMs in vascular cells and their therapeutic effects in the vascular remodeling associated with different cardiovascular diseases.
Mitochondrial Dysfunction and Acute Fatty Liver of Pregnancy
Authors: Ramanathan Raghu , Ibdah Jamal A.
Published: March 2022

The liver is one of the richest organs in mitochondria, serving as a hub for key metabolic pathways such as β-oxidation, the tricarboxylic acid (TCA) cycle, ketogenesis, respiratory activity, and adenosine triphosphate (ATP) synthesis, all of which provide metabolic energy for the entire body. Mitochondrial dysfunction has been linked to subcellular organelle dysfunction in liver diseases, particularly fatty liver disease. Acute fatty liver of pregnancy (AFLP) is a life-threatening liver disorder unique to pregnancy, which can result in serious maternal and fetal complications, including death. Pregnant mothers with this disease require early detection, prompt delivery, and supportive maternal care. AFLP was considered a mysterious illness and though its pathogenesis has not been fully elucidated, molecular research over the past two decades has linked AFLP to mitochondrial dysfunction and defects in fetal fatty-acid oxidation (FAO). Due to deficient placental and fetal FAO, harmful 3-hydroxy fatty acid metabolites accumulate in the maternal circulation, causing oxidative stress and microvesicular fatty infiltration of the liver, resulting in AFLP. In this review, we provide an overview of AFLP and mitochondrial FAO followed by discussion of how altered mitochondrial function plays an important role in the pathogenesis of AFLP.
Ascorbic Acid as an Adjuvant to Unbleached Cotton Promotes Antimicrobial Activity in Spunlace Nonwovens
Authors: Edwards Judson Vincent , Prevost Nicolette T. , Yager Dorne , Mackin Robert , Santiago Michael , Chang SeChin , Condon Brian , Dacorta Joseph
Published: March 2022

The development of affordable, effective, and environmentally friendly barrier fabrics is a current goal in antimicrobial textile development. The discovery of new routes to achieve non-toxic naturally occurring molecules with antimicrobial activity is of interest in the development of materials that promote wound healing, improve hygiene, and offer protection against nosocomial infection. Highly cleaned and sterile unbleached cotton has constituents that produce hydrogen peroxide at levels commensurate with those that favor cell signaling in wound healing. Here, we show the antimicrobial and antiviral properties of spunlaced griege cotton-containing nonwovens treated with ascorbic acid formulations. The mechanism of action occurs through the promotion of enhanced hydrogen peroxide activity. The levels of hydrogen peroxide activity afford antimicrobial activity against Gram-negative and Gram-positive bacteria and antiviral activity against MS2 bacteriophages. Spun-bond nonwoven unbleached cotton was treated with ascorbic acid using traditional pad-dry-cure methods. An assessment of antibacterial and antiviral activity against Staphylococcus aureus, Klebsiella pneumoniae, and MS2 bacteriophages with the AATCC 100 test method showed a 99.99% inhibitory activity. An approach to the covalent attachment of ascorbic to cellulose through citric acid crosslinking chemistry is also discussed. Thus, a simple, low-cost approach to antimicrobial and antiviral cotton-based nonwovens applicable to dressings, nosocomial barrier fabrics, and face masks can be adopted by combining ascorbic acid with spunlace greige cotton nonwoven fabrics.
The Effect of Discharge Mode on the Distribution of Myocardial Pulsed Electric Field—A Simulation Study for Pulsed Field Ablation of Atrial Fibrillation
Authors: Ji Xingkai , Zhang Hao , Zang Lianru , Yan Shengjie , Wu Xiaomei
Published: March 2022

Background: At present, the effects of discharge modes of multielectrode catheters on the distribution of pulsed electric fields have not been completely clarified. Therefore, the control of the distribution of the pulsed electric field by selecting the discharge mode remains one of the key technical problems to be solved. Methods: We constructed a model including myocardium, blood, and a flower catheter. Subsequently, by setting different positive and ground electrodes, we simulated the electric field distribution in the myocardium of four discharge modes (A, B, C, and D) before and after the catheter rotation and analyzed their mechanisms. Results: Modes B, C, and D formed a continuous circumferential ablation lesion without the rotation of the catheter, with depths of 1.6 mm, 2.7 mm, and 0.7 mm, respectively. After the catheter rotation, the four modes could form a continuous circumferential ablation lesion with widths of 10.8 mm, 10.6 mm, 11.8 mm, and 11.5 mm, respectively, and depths of 5.2 mm, 2.7 mm, 4.7 mm, and 4.0 mm, respectively. Conclusions: The discharge mode directly affects the electric field distribution in the myocardium. Our results can help improve PFA procedures and provide enlightenment for the design of the discharge mode with multielectrode catheters.
Fostering Offshore Wind Integration in Europe through Grid Connection Impact Assessment
Authors: Amaro Nuno , Egorov Aleksandr , Glória Gonçalo
Published: March 2022

Floating offshore wind energy is one of the solutions that can foster the ongoing climate transition in Europe. The ARCWIND project aims to contribute to this topic by considering multiple research activities designed to contribute to the development of multiple floating technologies, identifying high-potential deployment areas while considering their economic viability and the impact that these would have in existing power systems. Regarding the latter activity, a two-step methodology was implemented to first calculate the nodal capacity that existing electricity networks have to absorb energy from these potential new wind farms and secondly to assess the impact at the point of connection. This assessment is performed by identifying grid reinforcement needs, verifying the impact on short circuit current levels and measuring the impact on the existing energy mix at countrywide level. This article includes the description of this methodology as well as its application to six different use cases covering five European countries: Portugal, Spain, France, the United Kingdom, and Ireland. Results obtained seem to indicate that, in most cases, the current power systems have enough capacity for the possible connection of new floating offshore wind farms without major reinforcement needs and that these wind farms can have a major contribution to the countries’ energy mix and to the achievement of established climate targets.
Research on Construction Land Use Benefit and the Coupling Coordination Relationship Based on a Three-Dimensional Frame Model—A Case Study in the Lanzhou-Xining Urban Agglomeration
Authors: Zhang Weiping , Shi Peiji , Tong Huali
Published: March 2022

Coordinating the social, economic, and eco-environmental benefits of construction land use has become the key to the high-quality development of Lanzhou-Xining urban agglomerations (LXUA). Therefore, based on the coupling coordination connotation and interaction mechanism of construction land use benefit (CLUB), we measured the CLUB level and the coupling coordination degree (CCD) between its principal elements in LXUA from 2005 to 2018. Results showed that: (1) The construction land development intensity (CLDI) in the LXUA is generally low, and spatially presents a dual-core structure with Lanzhou and Xining urban areas as the core. (2) The comprehensive construction land use benefit has increased over time, but the overall level is not high. The spatial differentiation is obvious, and the core cities (Lanzhou and Xining) are significantly higher than other cities. (3) The regional differences in the subsystem benefit of construction land use are obvious. The social benefit and economic benefit showed a “convex” shape distribution pattern of “high in the middle and low in the east and west wings”, and regional differences of economic benefit vary greatly. The eco-environmental benefit was relatively high, showed a “concave” shape evolution in the east–west direction. (4) In addition, the CCD of the CLUB were still at a medium–low level. The higher the administrative level of the city, the better the economic foundation, and the higher or better the CCD of the social, economic, and eco-environmental benefits. (5) The CCD is inseparable from the influence of the three benefits of construction land use. Therefore, different regions should form their own targeted development paths to promote the coordinated and orderly development of LXUA.
Advances in Nanostructures for Antimicrobial Therapy
Authors: Jampilek Josef , Kralova Katarina
Published: March 2022

Microbial infections caused by a variety of drug-resistant microorganisms are more common, but there are fewer and fewer approved new antimicrobial chemotherapeutics for systemic administration capable of acting against these resistant infectious pathogens. Formulation innovations of existing drugs are gaining prominence, while the application of nanotechnologies is a useful alternative for improving/increasing the effect of existing antimicrobial drugs. Nanomaterials represent one of the possible strategies to address this unfortunate situation. This review aims to summarize the most current results of nanoformulations of antibiotics and antibacterial active nanomaterials. Nanoformulations of antimicrobial peptides, synergistic combinations of antimicrobial-active agents with nitric oxide donors or combinations of small organic molecules or polymers with metals, metal oxides or metalloids are discussed as well. The mechanisms of actions of selected nanoformulations, including systems with magnetic, photothermal or photodynamic effects, are briefly described.
Self-Healing Systems in Silicon Anodes for Li-Ion Batteries
Authors: Yuca Neslihan , Kalafat Ilknur , Guney Emre , Cetin Busra , Taskin Omer S.
Published: March 2022

Self-healing is the capability of materials to repair themselves after the damage has occurred, usually through the interaction between molecules or chains. Physical and chemical processes are applied for the preparation of self-healing systems. There are different approaches for these systems, such as heterogeneous systems, shape memory effects, hydrogen bonding or covalent–bond interaction, diffusion, and flow dynamics. Self-healing mechanisms can occur in particular through heat and light exposure or through reconnection without a direct effect. The applications of these systems display an increasing trend in both the R&D and industry sectors. Moreover, self-healing systems and their energy storage applications are currently gaining great importance. This review aims to provide general information on recent developments in self-healing materials and their battery applications given the critical importance of self-healing systems for lithium-ion batteries (LIBs). In the first part of the review, an introduction about self-healing mechanisms and design strategies for self-healing materials is given. Then, selected important healing materials in the literature for the anodes of LIBs are mentioned in the second part. The results and future perspectives are stated in the conclusion section.
Fiber Thickness and Porosity Control in a Biopolymer Scaffold 3D Printed through a Converted Commercial FDM Device
Authors: Lovecchio Joseph , Cortesi Marilisa , Zani Marco , Govoni Marco , Dallari Dante , Giordano Emanuele
Published: March 2022

3D printing has opened exciting new opportunities for the in vitro fabrication of biocompatible hybrid pseudo-tissues. Technologies based on additive manufacturing herald a near future when patients will receive therapies delivering functional tissue substitutes for the repair of their musculoskeletal tissue defects. In particular, bone tissue engineering (BTE) might extensively benefit from such an approach. However, designing an optimal 3D scaffold with adequate stiffness and biodegradability properties also guaranteeing the correct cell adhesion, proliferation, and differentiation, is still a challenge. The aim of this work was the rewiring of a commercial fuse deposition modeling (FDM) 3D printer into a 3D bioplotter, aiming at obtaining scaffold fiber thickness and porosity control during its manufacturing. Although it is well-established that FDM is a fast and low-price technology, the high temperatures required for printing lead to limitations in the biomaterials that can be used. In our hands, modifying the printing head of the FDM device with a custom-made holder has allowed to print hydrogels commonly used for embedding living cells. The results highlight a good resolution, reproducibility and repeatability of alginate/gelatin scaffolds obtained via our custom 3D bioplotter prototype, showing a viable strategy to equip a small-medium laboratory with an instrument for manufacturing good-quality 3D scaffolds for cell culture and tissue engineering applications.
Quad-Level Cell Switching with Excellent Reliability in TiN/AlOx:Ti/TaOx/TiN Memory Device
Authors: Shin Hee Ju , Seo Hyun Kyu , Lee Su Yeon , Park Minsoo , Park Seong-Geon , Yang Min Kyu
Published: March 2022

TiN/AlOx:Ti/TaOx/TiN memory devices using bilayer resistive switching memory demonstrated excellent durability and capability of QLC (quad-level cell) memory devices. The best nonvolatile memory characteristics with the lowest operation current and optimized 4 bit/cell states were obtained using the Incremental Step Pulse Programming (ISPP) algorithm in array. As a result, a superior QLC reliability (cycle endurance > 1 k at each level of the QLC, data retention > 2 h at 125 °C) for all the 4 bits/cell operations was achieved in sub-μm scaled RRAM (resistive random access memory) devices.
Dynamic NVH Numerical Analysis of Power Steering in the Presence of Lubricant in the System
Authors: Pietrusiak Damian , Wróbel Jakub , Czechowski Mateusz , Fiebig Wiesław
Published: March 2022

The ongoing shift towards hybrid and electric vehicles has a strong impact on noise and vibration engineering. New, complex dynamic phenomena are brought to vehicle user attention due to the absence of internal combustion engines and the significant role in vehicle and drive feel perception. This paper presents an FEM (Finite Element Method) dynamic simulation model of an automotive Electric Power Steering assembly. Preliminary modal simulations and experiments as well as field data replication techniques were implemented to identify the phenomena and prepare and validate model components. A full dynamic model of an Electric Power Steering was presented, and fine-tuned including the presence of lubrication at the gear mesh interface. Experimental investigations were conducted alongside FEM simulations for various model setups. Linear and nonlinear contact stiffness models were implemented, as well as contact damping, and simulated at chosen assembly interfaces. The results indicated that in the case of NVH (Noise Vibration and Harshness) analysis of shock/impact originating problems, contact parameters used for static, quasi-static, and low velocity analyses were not applicable. Nonlinear and damped contact stiffness provided better results in such a case.
Biogas Pollution and Mineral Deposits Formed on the Elements of Landfill Gas Engines
Authors: Konkol Izabela , Cebula Jan , Świerczek Lesław , Piechaczek-Wereszczyńska Magdalena , Cenian Adam
Published: March 2022

Municipal landfills generate a significant amount of high-energy biogas, which can be used as a renewable gaseous fuel. However, it is necessary to improve the quality of this biogas due to the presence of various chemical compounds. The most common pollutants in landfill biogas include volatile compounds of silicon, sulphur, phosphorus and chlorine. The aforementioned elements, as well as other metals, were found both in the deposits and in the engine oil. The paper presents detailed characteristics of the solid residues formed in selected parts of gas engines powered by landfill biogas. Its elemental composition and morphology were investigated in order to determine the structure and influence of these deposits. In order to better understand the observed features, selected analyses were also conducted for biogas, engine oil and the condensate generated during biogas dewatering. It was found that the content of individual elements in samples collected from the same part of the gas engine but sourced from various landfills vary. The occurrence of elements in deposits, e.g., Mg, Zn, P and Cr, depends on the location of sampling sites and the type of engine. It was also observed that the deposits formed in parts that come into contact with both biogas and engine oil contain Ca or Zn, which can be related to biogas pollutants as well as different oil additives. The presence of Al, Fe, Cu, Cr, Sn or Pb in selected motor oil samples can be explained by the penetration of metallic abrasives, which confirms the abrasive properties of the formed deposits. The analysis of the characteristic deposits may contribute to the selection of an appropriate landfill biogas purification technology, thus reducing the operating costs of energy cogeneration systems. Finally, we highlight challenges for biogas purification processes and anticipate the direction of future work.
Experimental Analysis of Ductile Cutting Regime in Face Milling of Sintered Silicon Carbide
Authors: Groeb Marvin , Hagelüken Lorenz , Groeb Johann , Ensinger Wolfgang
Published: March 2022

In this study, sintered silicon carbide is machined on a high-precision milling machine with a high-speed spindle, closed-loop linear drives and friction-free micro gap hydrostatics. A series of experiments was undertaken varying the relevant process parameters such as feedrate, cutting speed and chip thickness. For this, the milled surfaces are characterized in a process via an acoustic emission sensor. The milled surfaces were analyzed via confocal laser scanning microscopy and the ISO 25178 areal surface quality parameters such as Sa, Sq and Smr are determined. Moreover, scanning electron microscopy was used to qualitatively characterize the surfaces, but also to identify sub-surface damages such as grooves, breakouts and pitting. Raman laser spectroscopy is used to identify possible amorphization and changes to crystal structure. We used grazing incidence XRD to analyze the crystallographic structure and scanning acoustic microscopy to analyze sub-surface damages. A polycrystalline diamond tool was able to produce superior surfaces compared to diamond grinding with an areal surface roughness Sa of below 100 nm in a very competitive time frame. The finished surface exhibits a high gloss and reflectance. It can be seen that chip thickness and cutting speed have a major influence on the resulting surface quality. The undamaged surface in combination with a small median chip thickness is indicative of a ductile cutting regime.
A Modified Power Family of Distributions: Properties, Simulations and Applications
Authors: Hussein Mohamed , Cordeiro Gauss M.
Published: March 2022

In this paper, we present a new class of distributions called the modified power family by adding an extra shape parameter. Some of its structural properties are derived. Three special cases of the new family are considered and estimated using the method of maximum likelihood. The validity of the method of maximum likelihood is illustrated via Monte Carlo simulations. The importance and flexibility of the new family are empirically illustrated, partly due to efficient modeling of several real data. We compare the proposed family with some distributions and special models generated from other classes using classical statistical measures.
Correction: Singh et al. An Experimental Investigation on the Material Removal Rate and Surface Roughness of a Hybrid Aluminum Metal Matrix Composite (Al6061/SiC/Gr). Metals 2021, 11, 1449
Authors: Singh Mandeep , Garg Harish Kumar , Maharana Sthitapragyan , Yadav Anchal , Singh Rasmeet , Maharana Pragyansu , Nguyen Tien V. T. , Yadav Sudesh , Loganathan M. K.
Published: March 2022

Harish Kumar Garg was not included as an author in the published article [...]
Comparing Carbon Origami from Polyaramid and Cellulose Sheets
Authors: Islam Monsur , Weidler Peter G. , Mager Dario , Korvink Jan G. , Martinez-Duarte Rodrigo
Published: March 2022

Carbon origami enables the fabrication of lightweight and mechanically stiff 3D complex architectures of carbonaceous materials, which have a high potential to impact a wide range of applications positively. The precursor materials and their inherent microstructure play a crucial role in determining the properties of carbon origami structures. Here, non-porous polyaramid Nomex sheets and macroporous fibril cellulose sheets are explored as the precursor sheets for studying the effect of precursor nature and microstructure on the material and structural properties of the carbon origami structures. The fabrication process involves pre-creasing precursor sheets using a laser engraving process, followed by manual-folding and carbonization. The cellulose precursor experiences a severe structural shrinkage due to its macroporous fibril morphology, compared to the mostly non-porous morphology of Nomex-derived carbon. The morphological differences further yield a higher specific surface area for cellulose-derived carbon. However, Nomex results in more crystalline carbon than cellulose, featuring a turbostratic microstructure like glassy carbon. The combined effect of morphology and glass-like features leads to a high mechanical stiffness of 1.9 ± 0.2 MPa and specific modulus of 2.4 × 104 m2·s−2 for the Nomex-derived carbon Miura-ori structure, which are significantly higher than cellulose-derived carbon Miura-ori (elastic modulus = 504.7 ± 88.2 kPa; specific modulus = 1.2 × 104 m2·s−2) and other carbonaceous origami structures reported in the literature. The results presented here are promising to expand the material library for carbon origami, which will help in the choice of suitable precursor and carbon materials for specific applications.
In Vitro and In Silico Based Approaches to Identify Potential Novel Bacteriocins from the Athlete Gut Microbiome of an Elite Athlete Cohort
Authors: Wosinska Laura , Walsh Calum J. , O’Connor Paula M. , Lawton Elaine M. , Cotter Paul D. , Guinane Caitriona M. , O’Sullivan Orla
Published: March 2022

Exercise reduces inflammation, fatigue, and aids overall health. Additionally, physical fitness has been associated with desirable changes in the community composition of the athlete gut microbiome, with health-associated taxa being shown to be increased in active individuals. Here, using a combination of in silico and in vitro methods, we investigate the antimicrobial activity of the athlete gut microbiome. In vitro approaches resulted in the generation of 284 gut isolates with inhibitory activity against Clostridioides difficile and/or Fusobacterium nucleatum, and the most potent isolates were further characterized, and potential bacteriocins were predicted using both MALDI-TOF MS and whole-genome sequencing. Additionally, metagenomic reads from the faecal samples were used to recover 770 Metagenome Assembled Genomes (MAGs), of which 148 were assigned to be high-quality MAGs and screened for the presence of putative bacteriocin gene clusters using BAGEL4 software, with 339 gene clusters of interest being identified. Class I was the most abundant bacteriocin class predicted, accounting for 91.3% of predictions, Class III had a predicted abundance of 7.5%, and Class II was represented by just 1% of all predictions.
Essential Oils and Hydrolates: Potential Tools for Defense against Bacterial Plant Pathogens
Authors: Proto Maria Rita , Biondi Enrico , Baldo David , Levoni Matilde , Filippini Gianfranco , Modesto Monica , Di Vito Maura , Bugli Francesca , Ratti Claudio , Minardi Paola , Mattarelli Paola
Published: March 2022

The essential oils (EOs) of Origanum compactum and Satureja montana chemotyped (CT) at carvacrol, two Thymus vulgaris CT at thujanol and thymol, and Hydrolates (Hys) of S. montana and Citrus aurantium var. amara were chosen for studying their bactericidal efficacy against few phytobacterial pathogens. The Minimal Inhibitory Concentration (MIC) and Bactericidal Concentration (MBC) were found by microdilution assay. The essential oils of O. compactum (MBC 0.06% v/v), T. vulgaris CT thymol (MBC 0.06% v/v), and Hy of C. aurantium (MBC 6.25% v/v) resulted in being the most effective against Erwinia amylovora; thus, they were used as starting concentrations for ex vivo assays. Despite the great in vitro effectiveness, the disease incidence and the population dynamic ex vivo assays showed no significant results. On the other hand, EO of O. compactum and Hy of C. aurantium (at 0.03% and 4.5% v/v, respectively) showed resistance induction in tomato plants against Xanthomonas vesicatoria infections; both treatments resulted in approximately 50% protection. In conclusion, EOs and Hys could be promising tools for agricultural defense, but further studies will be necessary to stabilize the EOs emulsions, while Hys application could be an effective method to prevent bacterial diseases when used as resistance inducer by pre-transplantation treatment at roots.
Predominance of Other Pathogenic Bacteria among Presumptive Tuberculosis Cases Attending Tuberculosis Clinics in Mwanza, Tanzania: A Cross-Sectional Laboratory-Based Study
Authors: Buchera Florencia S. , Silago Vitus , Japhet Geofrey , Mtemisika Conjester I. , Damiano Prisca , Nyawale Helmut A. , Mushi Martha F. , Mirambo Mariam M. , Seni Jeremiah , Mshana Stephen E.
Published: March 2022

This study was designed to determine the prevalence and co-infection of Mycobacterium tuberculosis and other pathogenic bacteria among presumptive cases of tuberculosis (TB) at selected hospitals in Mwanza, Tanzania. GeneXpert and conventional bacteriological culture and sensitivity were used for the detection of TB and other pathogenic bacteria, respectively. STATA version 13.0 was used for data analysis. The median (IQR) age of participants was 33 (19–51) years with males forming more than half (i.e., 59% (158/264)) of the participants. Microscopically, 29.5% (78/264) of the patients had polymorphonuclear leucocytes in the sputum samples. Approximately 7.2% (19/264), 16.3% (43/264), and 1.1% (3/264) of participants had TB, other pathogenic bacteria, and co-infections, respectively. One sample had growth of two other bacteria, resulting in a total of 44 isolated bacteria with the predominance of Gram-negative bacteria at 75.0% (33/44). The predominant species isolated was the Klebsiella pneumoniae complex at 52.3% (23/44). Overall, 27.3% (9/33) of GNB were resistant to third-generation cephalosporins, while Gram-positive bacteria were more resistant to erythromycin at 63.6% (7/11). Good quality sputa had a significantly higher yield of pathogenic bacteria than poor quality sputa (37.2% vs. 7.5%, p < 0.001). Presumptive TB cases were predominantly infected with other pathogenic bacteria than M. tuberculosis. Therefore, other pathogenic bacteria should be considered when attending presumptive TB cases to ensure favorable treatment outcomes.
Geologic Carbon Storage of Anthropogenic CO2 under the Colorado Plateau in Emery County, Utah
Authors: Moodie Nathan , Jia Wei , Middleton Richard , Yaw Sean , Lee Si-Yong , Xiao Ting , Wheatley David , Steele Peter , Esser Rich , McPherson Brian
Published: March 2022

Geologic Carbon Storage (GCS) is a promising technology for storing large volumes of anthropogenic CO2 effectively and permanently. Numerical simulations are an integral part of site selection and characterization for any potential GCS site. As part of the DOE-funded CarbonSAFE Rocky Mountains Phase I project, a regional GCS analysis was undertaken to understand the efficacy of storing CO2 emissions from the power generation and heavy industry in central Utah’s favorable geology. In this study, the injection of CO2 for geologic storage was simulated in the Navajo Sandstone Formation in Emery County, Utah. Carbon dioxide was sourced from regional power generation stations and heavy industries throughout Utah, with an emphasis on emissions reduction at the Hunter Power Plant near Castle Dale, Utah. A simulation grid was extracted from the project’s geological model encompassing an area around Price, Huntington, and Castle Dale in central Utah. The Navajo Sandstone Member of the Glen Canyon Group was the target of CO2 injection with the overlying Carmel formation providing the primary seal. A suite of simulations was performed assessing the viability of this area for permanent CO2 storage. Results indicate that the area can not only store 46 million metric tons of anthropogenic CO2, meeting the project goals, but this area has the capacity to securely store at least 1.3 billion tons of CO2, suggesting the injection site and surrounding geology are suitable locations for commercial-scale GCS.
Kinetics of Ni, V and Fe Leaching from a Spent Catalyst in Microwave-Assisted Acid Activation Process
Authors: Wang Tian , Ren Jing , Ravindra Annavarapu V. , Lv Yan , Le Thiquynhxuan
Published: March 2022

Ni, V and Fe are the main contaminant metals that lead to the deactivation of the spent fluid catalytic cracking (SFCC) catalyst. In this work, the properties and distribution of Ni, V and Fe in the SFCC catalyst are investigated by employing EPMA-EDX, SEM and XPS techniques. The kinetics of Ni, V, Fe and Al leaching in organic and inorganic acids are studied under microwave heating. The EPMA-EDX results show that Fe and Ni mainly accumulate near the particle surface, while V eventually distributes throughout the catalyst particle. The XPS result suggests that the phase speciations of Ni in the SFCC catalyst are Ni, Ni2SiO4 and NiAl2O4, while Fe is present in a mixture of Fe3O4, Fe2O3 and Fe2SiO4. V is in the forms of V2O5 and VO2. Compared with oxalic acid, sulfuric acid has a better removal effect of contaminant metals, especially for Ni. The leaching kinetics results indicate that using either sulfuric acid or oxalic acid, the apparent activation energy of V is obviously lower than that of Fe and Ni, and the priority of the three contaminant metals in the removal effect is V > Fe > Ni. In addition, the leaching kinetics of contaminant metals in the microwave-assisted acid activation process are controlled by the surface chemical reaction control model.
Significant Enhancement of the Capacity and Cycling Stability of Lithium-Rich Manganese-Based Layered Cathode Materials via Molybdenum Surface Modification
Authors: Shao Yijia , Lu Zhiyuan , Li Luoqian , Liu Yanni , Yang Lijun , Shu Ting , Li Xiuhua , Liao Shijun
Published: March 2022

Lithium-rich manganese-based layered cathode materials are considered to be one of the best options for next-generation lithium-ion batteries, owing to their ultra-high specific capacity (>250 mAh·g−1) and platform voltage. However, their poor cycling stability, caused by the release of lattice oxygen as well as the electrode/electrolyte side reactions accompanying complex phase transformation, makes it difficult to use this material in practical applications. In this work, we suggest a molybdenum surface modification strategy to improve the electrochemical performance of Li1.2Mn0.54Ni0.13Co0.13O2. The Mo-modified Li1.2Mn0.54Ni0.13Co0.13O2 material exhibits an enhanced discharge specific capacity of up to 290.5 mAh·g−1 (20 mA·g−1) and a capacity retention rate of 82% (300 cycles at 200 mA·g−1), compared with 261.2 mAh·g−1 and a 70% retention rate for the material without Mo modification. The significantly enhanced performance of the modified material can be ascribed to the formation of a Mo-compound-involved nanolayer on the surface of the materials, which effectively lessens the electrolyte corrosion of the cathode, as well as the activation of Mo6+ towards Ni2+/Ni4+ redox couples and the pre-activation of a Mo compound. This study offers a facile and effective strategy to address the poor cyclability of lithium-rich manganese-based layered cathode materials.
Carnosic Acid Protects INS-1 β-Cells against Streptozotocin-Induced Damage by Inhibiting Apoptosis and Improving Insulin Secretion and Glucose Uptake
Authors: El-Huneidi Waseem , Anjum Shabana , Saleh Mohamed A. , Bustanji Yasser , Abu-Gharbieh Eman , Taneera Jalal
Published: March 2022

Carnosic acid (CA), a natural polyphenolic diterpene derived from Rosmarinus officinalis, has been proven to possess a broad spectrum of medicinal properties. Nevertheless, no studies on its impact on pancreatic β-cells have been conducted to date. Herein, clonal rat INS-1 (832/13) cells were pretreated with CA for 24 h and then incubated with streptozotocin (STZ) for 3 h. Several functional experiments were performed to determine the effect of CA on STZ-induced pancreatic β-cell damage, including cell viability assay, apoptosis analysis, and measurement of the level of insulin secretion, glucose uptake, malondialdehyde (MDA), reactive oxygen species (ROS), and proteins expression. STZ treatment decreased cell survival, insulin secretion, glucose uptake, and increased apoptosis, MDA, and ROS production in INS-1 cells. Furthermore, protein expression/phosphorylation analysis showed significant down-regulation in insulin, PDX-1, PI3K, AKT/p-AKT, and Bcl2. On the other hand, expression of BAX and BAD and cleaved PARP were significantly increased. Interestingly, preincubation with CA reversed the adverse impact of STZ at the cellular and protein expression levels. In conclusion, the data indicate that CA protects β-cells against STZ-induced damage, presumably through its modulatory effect on the different pathways, including the Pi3K/AKT/PDX-1/insulin pathway and mitochondria-mediated apoptosis.
Structure and Biological Activity of Ergostane-Type Steroids from Fungi
Authors: Zhabinskii Vladimir N. , Drasar Pavel , Khripach Vladimir A.
Published: March 2022

Mushrooms are known not only for their taste but also for beneficial effects on health attributed to plethora of constituents. All mushrooms belong to the kingdom of fungi, which also includes yeasts and molds. Each year, hundreds of new metabolites of the main fungal sterol, ergosterol, are isolated from fungal sources. As a rule, further testing is carried out for their biological effects, and many of the isolated compounds exhibit one or another activity. This study aims to review recent literature (mainly over the past 10 years, selected older works are discussed for consistency purposes) on the structures and bioactivities of fungal metabolites of ergosterol. The review is not exhaustive in its coverage of structures found in fungi. Rather, it focuses solely on discussing compounds that have shown some biological activity with potential pharmacological utility.
Enhancement of the Anti-Inflammatory Activity of NSAIDs by Their Conjugation with 3,4,5-Trimethoxybenzyl Alcohol
Authors: Tziona Paraskevi , Theodosis-Nobelos Panagiotis , Papagiouvannis Georgios , Petrou Anthi , Drouza Chryssoula , Rekka Eleni A.
Published: March 2022

The synthesis of derivatives of three nonspecific COX-1 and COX-2 inhibitors, ibuprofen, ketoprofen, naproxen is presented. These acids were connected via an amide bond with an amino acid (L-proline, L-tyrosine, and beta-alanine) used as a linker. The amino acid carboxylic group was esterified with 3,4,5 trimethoxybenzyl alcohol. The activity of the novel derivatives was examined in vivo on carrageenan-induced inflammation, and in vitro, as cyclooxygenase and lipoxygenase inhibitors. It was found that the new compounds were more potent anti-inflammatory agents than the parent drugs. Thus, the ibuprofen (21) and ketoprofen (16) derivatives reduced rat paw edema by 67 and 91% (the reduction by the relevant NSAIDs was 36 and 47%, respectively). They inhibited COX-2 more than the starting drugs (21 by 67%, ibuprofen 46%, 19 by 94%, ketoprofen 49%). Docking of compounds on the active sites of COX-1 and COX-2 reflects their in vitro activity. Thus, 19 adopts an unfavorable orientation for COX-1 inhibition, but it binds effectively in the binding pocket of COX-2, in agreement with the absence of activity for COX-1 and the high inhibition of COX-2. In conclusion, the performed structural modifications result in the enhancement of the anti-inflammatory activity, compared with the parent NSAIDs.
Atomistic Simulation of Lysozyme in Solutions Crowded by Tetraethylene Glycol: Force Field Dependence
Authors: Liu Donglin , Qiu Yejie , Li Qing , Zhang Haiyang
Published: March 2022

The behavior of biomolecules in crowded environments remains largely unknown due to the accuracy of simulation models and the limited experimental data for comparison. Here we chose a small crowder of tetraethylene glycol (PEG-4) to investigate the self-crowding of PEG-4 solutions and molecular crowding effects on the structure and diffusion of lysozyme at varied concentrations from dilute water to pure PEG-4 liquid. Two Amber-like force fields of Amber14SB and a99SB-disp were examined with TIP3P (fast diffusivity and low viscosity) and a99SB-disp (slow diffusivity and high viscosity) water models, respectively. Compared to the Amber14SB protein simulations, the a99SB-disp model yields more coordinated water and less PEG-4 molecules, less intramolecular hydrogen bonds (HBs), more protein–water HBs, and less protein–PEG HBs as well as stronger interactions and more hydrophilic and less hydrophobic contacts with solvent molecules. The a99SB-disp model offers comparable protein–solvent interactions in concentrated PEG-4 solutions to that in pure water. The PEG-4 crowding leads to a slow-down in the diffusivity of water, PEG-4, and protein, and the decline in the diffusion from atomistic simulations is close to or faster than the hard sphere model that neglects attractive interactions. Despite these differences, the overall structure of lysozyme appears to be maintained well at different PEG-4 concentrations for both force fields, except a slightly large deviation at 370 K at low concentrations with the a99SB-disp model. This is mainly attributed to the strong intramolecular interactions of the protein in the Amber14SB force field and to the large viscosity of the a99SB-disp water model. The results indicate that the protein force fields and the viscosity of crowder solutions affect the simulation of biomolecules under crowding conditions.
High Performance Polycarbonate Nanocomposites Mechanically Boosted with Titanium Carbide in Material Extrusion Additive Manufacturing
Authors: Vidakis Nectarios , Petousis Markos , Grammatikos Sotirios , Papadakis Vassilis , Korlos Apostolos , Mountakis Nikolaos
Published: March 2022

Herein, a polycarbonate (PC) polymer is melt extruded together with titanium carbide (TiC) nano powder for the development of advanced nanocomposite materials in material extrusion (MEX) 3D printing. Raw material for the 3D printing process was prepared in filament form with a thermomechanical extrusion process and specimens were built to be tested according to international standards. A thorough mechanical characterization testing course (tensile, flexural, impact, microhardness, and dynamic mechanical analysis-DMA) was conducted on the 3D printed specimens. The effect of the ceramic filler loading was also investigated. The nanocomposites’ thermal and stoichiometric properties were investigated with thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), energy-dispersive X-ray spectroscopy (EDS), and Raman respectively. The specimens’ 3D printing morphology, quality, and fracture mechanism were investigated with atomic force microscopy (AFM) and scanning electron microscopy (SEM) respectively. The results depicted that the addition of the filler decidedly enhances the mechanical response of the virgin polymer, without compromising properties such as its processability or its thermal stability. The highest improvement of 41.9% was reported for the 2 wt.% filler loading, making the nanocomposite suitable for applications requiring a high mechanical response in 3D printing, in which the matrix material cannot meet the design requirements.
Characterizations of MWCNTs Nanofluids on the Effect of Surface Oxidative Treatments
Authors: Mohd Saidi Norshafiqah , Norizan Mohd Nurazzi , Abdullah Norli , Janudin Nurjahirah , Kasim Noor Azilah Mohd , Osman Mohd Junaedy , Mohamad Imran Syakir
Published: March 2022

In this study, multi-walled carbon nanotubes (MWCNTs) were chemically modified using three acid treatment methods to introduce the surface oxygen functional group (SOFG). The presence of SOFG on the MWCNTs has been characterized by Fourier Transform Infrared (FTIR) spectroscopy. Morphology, structural and thermal properties were performed using Field Emission Scanning Electron Microscopy (FESEM), Raman spectroscopy, and Thermogravimetric analysis (TGA), respectively. The result shows that the modification with acid treatment significantly affects the degree of defects and surface group functionality of surface oxidized MWCNTs from method B. The preparation of nanofluids using MWCNTs produced from method B (MWCNT-MB) was prepared using two different parameters: with and without polyvinylpyrrolidone (PVP) as surfactant. The experiment was conducted by setting variable carbon particle concentration from 0.1 wt.% to 1.0 wt.%, and the amount of PVP is 10% of carbon particles at different temperatures (6 °C, 25 °C, 40 °C). Based on visual observation, the dispersion of carbon particles was enhanced by the presence of PVP as the stabilizing agent. The thermal conductivity performance of nanofluids revealed that the surface oxidized MWCNTs with PVP show enhanced thermal conductivity compared to the nanofluid containing MWCNTs without PVP. The improvement contributes to this in terms of stability and homogenization of nanoparticles. Hence the improved distribution of MWCNTs in water-based media improves thermal conductivity. These promising properties of MWCNTs in water-based fluids would enable the nanofluids to be used in heat transfer fluid and cooling applications.
Antioxidant and Anti-Inflammatory Properties of Rubber Seed Oil in Lipopolysaccharide-Induced RAW 267.4 Macrophages
Authors: Liu Jing , Zhao Lulu , Cai Hongying , Zhao Zitao , Wu Yongbao , Wen Zhiguo , Yang Peilong
Published: March 2022

Rubber seed oil (RSO) is a typical PUFA-enriched plant oil, but it has not been widely used as a healthy edible oil resource due to the lack of understanding of its nutritional values, health biological effects, and action mechanisms. This work was conducted to characterize the basic physicochemical properties, evaluate the antioxidant and anti-inflammatory properties, and explore the involved mechanisms of RSO in LPS-induced RAW 264.7 cells. In the present study, the basic physicochemical parameters of RSO indicated that RSO has good qualities as a potential edible plant oil resource. In LPS-induced macrophages, RSO supplementation displayed a significant antioxidant effect by decreasing ROS and MDA levels as well as elevating T-AOC. In addition, RSO supplementation showed an anti-inflammatory effect by reducing the production of NO, IL-1β, IL-6, and TNF-α while promoting the production of IL-10. Moreover, RSO supplementation decreased the mRNA expression of IL-6, IL-1β, TNF-α, iNOS, and MCP-1 genes while increasing the mRNA expression of the IL-10 gene. Furthermore, RSO supplementation increased Nrf2 protein expression and up-regulated antioxidant genes (HO-1 and NQO-1), which was accompanied by the decrease in TLR4 protein expression and NF-κB p65 phosphorylation as well as IκBα phosphorylation. This study provided some insight into the applications of RSO as a healthy edible oil resource.