bluuebunny/crossref_metadata_2025 huggingface dataset:
Maternal outcomes of rheumatic heart disease in pregnancy
Authors: L. Hema Priya , Bhandiwad Ambarish , Desai Nagaraj , Kondareddy Triveni
Published: February 2017

Background: Preexisting cardiac disease is seen in 1-3% of pregnancies. In developing countries, sequelae of rheumatic fever often constitute the majority of women with heart disease; whereas in developed countries, it is the congenital heart diseases. The aim of this study was to examine the changing trends and mode of care of women with Rheumatic heart disease in pregnancy over a period of five years in a tertiary care centre.Methods: Patient records over five years were retrieved and maternal and perinatal outcomes were documented. The present study reports the outcomes of 72 women with rheumatic heart disease.Results: The prevalence of heart disease in pregnancy in our study was 1.72%. The prevalence was higher among the rural population, and in lower socio economic strata. 30% of patients were diagnosed during pregnancy. The risk of complications co - related with their functional status at the onset of pregnancy. The mode of termination of pregnancy and indications for LSCS did not vary. However, the risk of complications was greatest during labour and post-partum period. The mean birth weight was 2.7 kg, however, 30% of term neonates were of low birth weight (<2.5 kg).Conclusions: Rheumatic heart disease continues to be a major cause of cardiac disease complicating pregnancy. However, early diagnosis, appropriate management prior to pregnancy, and good functional status at the time of entering pregnancy allowed for a good maternal and neonatal outcome.
A study on faeto-maternal outcome in pregnancy with jaundice due to hepatitis E
Authors: Jena Sushanta Kumar , Mohanty Minakshi , Behuria Sasmita
Published: February 2017

Background: Hepatitis E virus is a non-envelope RNA virus responsible for large epidemics in Asia, the Middle east Mexico and Africa. It spreads via the feco-oral route, and has an incubation period of 8-10 weeks. The objective of the study was to find out the effect of hepatitis E during pregnancy on faeto-maternal outcome.Methods: A study on faeto-maternal outcome of 38 pregnant women admitted with jaundice due to hepatitis E to the Department of Hepatology, Shri Ramachandra Bhanj (SCB) Medical College, Cuttack, Odisha, India during January 2015 to December 2015.Results: 38 Pregnant women admitted to the Department of Hepatology of SCB Medical College, Cuttack, Odisha, India were studied. 65.7% were from rural area, 92.1% were in their third trimester of pregnancy. Serum bilirubin >10 mg in 52.19 %, SGOT, SGPT and alkaline phosphatase were raised in majority of them. Out of 38 women 32 delivered and 6 remain undelivered. In 81.25% of cases labor was spontaneous, perinatal mortality was observed in 46.87% of cases and 5 women died during child birth.Conclusions: When pregnancy is associated with jaundice due to hepatitis E, there is high perinatal and maternal morbidity and mortality. Early diagnosis and careful management is required for such cases.
Changing trends in genital cancer
Authors: Singh Abha , Kujur Avinashi
Published: February 2017

Background: Globally, cancers in all forms are causing about 12% of all deaths. In developed countries cancer is the second leading cause of death accounting for 21% of mortality by other causes and in developing countries it ranks third, accounting for 9.5% of all deaths. The objective was to find the incidence of genital malignancy and any change in the trend at Pandit Jawahar Lal Nehru Memorial Medical College, Raipur, Chhattisgarh, India.Methods: This was a cross-sectional, retrospective, observational study conducted in Dr. BRAM Hospital, Department of Obstetrics and Gynecology from 2000 to 2015. We analyzed data for demographic profile, type of the genital carcinoma and the stages in which they reported and observed any changing trends.Results: Over 15 years the contribution of genital cancer remained almost stable at 2.78% in 2000 to 2.48% in 2015. 85% women belonged to rural residence, 75% were educated up to high school level and 76% women were from low socioeconomic status. Maximum prevalence of genital cancer is 65.90% falls in 45-60 years age group. Young age at first sexual activity, low education, parity >4 were found to be associated risk factors. Most significant changing trend was that the incidence of cancer cervix decreased by 11%. There was a shift from stage III to stage II at the time of reporting. The cancer endometrium emerged as new site for genital cancer with increased incidence.Conclusions: Cervical cancers are the most common cause of mortality but it is a preventable disease. Its decreasing trend and shift to early stage diagnosis is a positive beginning.
Towards critical global education worker subjectivity: An exploration of narratives of American women engaged in education-related international volunteerism
Authors: Mule Lucy
Published: February 2017

International volunteerism is increasingly associated with shaping global subjectivities of participants. Significant numbers of Global North volunteers – whether working through established volunteer organizations, corporations, nonprofits, academia, or personal networks and
connections – engage in educationrelated activities while in the Global South. I emphasize in this paper that education-related international volunteering presents a rich context in which to explore global subjectivities due to the high likelihood of participants' engagement with mobility,
difference, poverty, inequality, and development. In this paper, I explore six women's accounts of their transnational experiences and resulting understandings of their education-related work. Four related thematic categories derived from these accounts convey meanings of education-related
work in terms of self-fulfilment, social responsibility, active engagement with host communities, and cross-cultural competence. I explore two overlapping subjectivities – participatory and critical – that emerge from an exploration of these themes, examine how they intersect with
common discourses of international volunteering and development, and discuss the implications for the relationship between global citizenship and education-related international volunteering.
Development of a Database for Study Data in Registration Applications for Veterinary Medicinal Products
Authors: Finnah Anke , Klee Sabine , Ruddat Inga , Schneider Bettina , Kreienbrock Lothar
Published: February 2017

Objective: In the present study, the feasibility of a systematic record of clinical study data from marketing authorisation applications for veterinary medicinal products (VMP) and benefits of the selected approach were investigated.

Background: Drug registration dossiers for veterinary medicinal products contain extensive data from drug studies, which are not easily accessible to assessors.

Evidentiary value: Fast access to these data including specific search tools could facilitate a meaningful use of the data and allow assessors for comparison of test and studies from different dossiers.

Methods: First, pivotal test parameters and their mutual relationships were identified. Second, a data model was developed and implemented in a relational database management system, including a data entry form and various reports for database searches. Compilation of study data in the database was demonstrated using all available clinical studies involving VMPs containing the anthelmintic drug Praziquantel.
 By means of descriptive data analysis possibilities of data evaluation including graphical presentation were shown. Suitability of the database to support the performance of meta-analyses was tentatively validated.

Results: The data model was designed to cover the specific requirements arising from study data. A total of 308 clinical studies related to 95 VMPs containing Praziquantel (single agent and combination drugs) was selected for prototype testing. The relevant data extracted from these studies were appropriately structured and shown to be basically suitable for descriptive data analyses as well as for meta-analyses.

Conclusion: The database-supported collection of study data would provide users with easy access to the continuously increasing pool of scientific information held by competent authorities. It enables specific data analyses. Database design allows expanding the data model to all types of studies and classes of drugs registered in veterinary medicine. The needs for detailed data recording and versatility of the data model must be carefully balanced.

Application: The database will be used by regulatory authorities.
Screening of probiotics bacteria from coral reef using co-culture method for controlling vibriosis in tiger shrimp (Penaeus monodon) larvae
Authors: Sasanti Ade Dwi , . Widanarni , . Sukenda
Published: February 2017

ABSTRACTThis study was carried out to obtain bacteria isolates from coral reef using co-culture method which potentially inhibit Vibrio harveyi growth. A total of 110 isolates were isolated from Acropora sp., Merulina sp., Hystrix sp., Poecillophora sp., Porites sp., and Haliophora sp., and were screened for their antagonistic activity against V. harveyi in in vitro and in vivo test. Five candidate probiotics (5H1 candidate probiotics isolated from Acropora sp., 11I and 11G isolates isolated from Hystrix sp. and 13B and 13G1 isolates isolated from Poecillophora sp., was able to inhibit growth of V. harveyi MR5339 RFR up to 101‒102 cfu/mL. Two isolates (13B and 13G1) were not pathogenic at concentration 106 cfu/mL bacteria and could increase of survival rate of tiger shrimp (Penaeus monodon) larvae in in vivo test. Survival rate of tiger shrimp larvae that treatment with 13B and 13G1 were 86,67% and 88,33%, and have a significant different with positive control (61,67%). Partial sequencing of 16S-rRNA showed that 13G1 isolate was similar to V. alginolyticus.Keywords: vibriosis, Vibrio harveyi, tiger shrimp, probiotic, coral reefABSTRAKPenelitian ini bertujuan untuk mendapatkan bakteri probiotik asal terumbu karang dengan metode kultur bersama untuk pengendalian penyakit vibriosis pada larva udang windu (Penaeus monodon). Sebanyak 110 isolat berhasil diisolasi dari Acropora sp., Merulina sp., Hystrix sp., Poecillophora sp., Porites sp., dan Heliophora sp. dan dilakukan penapisan untuk melihat aktivitas kemampuannya melawan Vibrio harveyi MR 5339 RfR dalam uji in vitro dan in vivo. Sebanyak 56 isolat menghasilkan daya hambat terhadap V. harveyi MR5339 RfR pada metode kultur bersama. Lima isolat kandidat probiotik (isolate 5H1 diisolasi dari Acropora sp., isolat 11I dan 11G diisolasi dari Hystrix sp., serta isolat 13B dan 13G1 yang diisolasi dari Poecillophora sp.), mampu menekan pertumbuhan V. harveyi MR5339 RfR hingga 101–102 cfu/mL. Kedua isolat (13B dan 13G1) terbukti tidak bersifat patogen pada konsentrasi 106 cfu/mL dan mampu meningkatkan sintasan larva udang windu pada uji in vivo. Nilai sintasan larva pada perlakuan yang diberi kandidat probiotik 13B dan 13G1 berturut-turut adalah 86,67% dan 88,33%, namun berbeda nyata dengan kontrol positif (61,67%). Hasil analisis sekuen sebagian gen 16S-rRNA menunjukkan bahwa isolat 13G1 termasuk spesies V. algynoliticus.Kata kunci: vibriosis, Vibrio harveyi, udang windu, probiotik, terumbu karang
The use of sand as a substrate for culture of polychaeta worm Dendronereis pinnaticirris
Authors: Mustofa Ahmad Ghufron , Harris Enang , Supriyono Eddy , Jusadi Dedi
Published: February 2017

This experiment was conducted to study the activity, protein retention, daily growth rate, and production of Dendronereis pinnaticirris cultured in different soil substrates. Forty tested worms with average body weight of 150 mg were adapted in laboratory for 30 days. Thereafter, worms were cultured for 30 days in the 13.6 L aquaria and equipped with aeration system. A triplicate experiment was conducted using 10 cm depth of sterilized soil substrate with particle diameter of either 63‒250 μm, 250‒500 μm, or without substrate. The results showed that (1) D. pinnaticirris always swims actively when cultured in the medium without substrate, thereby resulting into the mass mortality (96.7%); (2) the substrate with particle diameter of 63‒250 μm generated significantly higher daily growth rate, survival rate, and production of D. pinnaticirris, but protein retention and feed efficiency were insignificance with those cultured in the substrate of 250–500 μm.

Keywords: substrate, protein retention, production, Dendronereis pinnaticirris
Duration of feed application of mixed powders of Phyllanthus niruri and Allium sativum for the prevention of Aeromonas hydrophila infection in catfish Clarias sp.
Authors: Wahjuningrum Dinamella , Widiani Iis , Nuryati Sri
Published: February 2017

Motile Aeromonad Septicaemia (MAS) caused by Aeromonas hydrophila induced serious epidemics of disease in catfish Clarias sp. The purposed of this research was to determine optimum durations of feed application of mixed powders Phyllanthus niruri and Allium sativum to prevent MAS. Experimental design that used was Complete Random Design which consist of three treatments and three replications. This research tested on three different of durations of feed application of P. niruri and A. sativum, namely A (powder of P. niruri and A. sativum feed application during 21 days and infected with A. hydrophila 0,1 mL IP), B (powder of P. niruri and A. sativum feed application during 14 days and injected IP with A. hydrophila 0,1 mL), C (powder of P. niruri and A. sativum feed application during seven days and injected IP with A. hydrophila 0,1 mL), and control, namely K- (without P. niruri and A. sativum feed application and injected IP with PBS 0,1 mL) and K+ (without P. niruri and A. sativum powder application and injected IP with A. hydrophila 0,1 mL). The treatments was given for 21 days before challenging test, at 22th day test in vivo carried out by injecting A. hydrophila (108 CFU/mL) into the fish by intramuscular and observed for 10 days. The highest value of survival rate that consist in treatment K- was 100±0.00%, treatment A was 93.3±11.55%, and treatment B was 73.33±30.55%. While treatment K+ and C have the same survival rate that is, 26.67±11.55%. The application P. niruri and A. sativum powder during 21 days was optimum for preventing MAS in catfish.

Keywords: Phyllanthus niruri, Allium sativum, Aeromonas hydrophila, Clarias sp.
Isolation and characterization of pathogenic Vibrio on tiger grouper Epinephelus fuscoguttatus
Authors: Ilmiah . , Sukenda . , Widanarni . , Harris Enang
Published: February 2017

This study was aimed to obtain pathogenic bacterial isolate causing vibriosis disease. Isolation of Vibrio was conducted from maribound tiger grouper collected from floating net cage in Barru Regency using TCBS medium. Ability to cause vibriosis was confirmed by pathogenicity test performed by mean injecting the tiger grouper juveniles with bacterial suspension at concentration of 106 CFU/fish and mortality of fish during seven days observation then was noted. Then, the Vibrio pathogenic isolate was characterized and identified based on morphology, growth, and biochemical features. Moreover, the most pathogenic isolate was identified by molecular analysis of 16S-rRNA gene sequences. The results showed that three potential isolates caused Vibriosis disease in tiger grouper culture. The isolates tested were biochemically identified as Vibrio metschnikovii, V. parahaemolyticus, and V. mimicus. The most virulent among isolates was V. parahaemolyticus.

Keywords: isolation, characterization, pathogenic, vibriosis, tiger grouper
Efficacy of DNA vaccine encoding koi herpesvirus glycoprotein GP-25in common carp juvenile by immersion
Authors: Nuswantoro Soko , Alimuddin . , Yuhana Munti , Santika Ayi , Nuryati Sri , Zainun Zakki , Mawardi Mira
Published: February 2017

Koi herpesvirus (KHV) is a herpesvirus that particularly infects and causes mass mortality to koi and common carp. Therefore, the protection of common carp from KHV infection is urgently needed. In this study, we developed an application of DNA vaccine encoding KHV glycoprotein-25 by immersion method to increase survival of common carp against KHV infection. A total of 400 common carp juveniles at 30-day-old were immersed in 1-L water containing 1.3×108CFU/mL of the killed Escherichia coli cells carrying DNA vaccine. Three frequencies and three duration of fish immersion were tested, namely: 1×30 minutes, 1×60 minutes, 1× 90 minutes, 2×90 minutes and 3×90 minutes by interval of 24 hours. Reversetranscription polymerase chain reaction analysis showed that DNA vaccine was successfully expressed in the vaccinated fish. Fish at twenty eight days post vaccination were challenged by injecting 10-4 mL of KHV per fish. The result showed that vaccination by 1×30 minutes immersion allowed 61% of fish survived, and this was significantly higher (p<0.05) compared to control (without vaccination), but it was similar among vaccination treatments (p>0.05). The relative percent survival of vaccinated fish were also similar among treatments (p>0.05). DNA vaccination has increased fish survival about two fold higher compared to unvaccinated fish control (26.67%). Thus, DNA vaccination was effectively delivered by immersion for 1×30 minutes, and this technique can be useful to level up the resistance of common carp juveniles against KHV infection.

Keywords: DNA vaccine, KHV, glycoprotein, immersion, common carp
Dietary vitamin E on the reproductive performance of the fantail goldfish Carassius auratus auratus
Authors: Arfah Harton , Melati . , Setiawati Mia
Published: February 2017

ABSTRACT

 

This research was conducted to examine the different dose of vitamin E in the diet on female broodstock reproduction performance of the fantail goldfish Carassius auratus auratus. This research consisted of four treatments with three replications. The use of vitamin E doses was 0, 125, 250, and 375 mg/kg. The vitamin E was dissolved in vegetable oil and mixed with albumen as a binder in feed. The vitamin E was sprayed at feed and was air dried. Female broodstock of the fantail goldfishes were reared for 40 days. The result showed that 375 mg/kg treatment performed the highest quality of reproduction. Egg diameter, gonadosomatic index, fecundity, and germinal vesicle breakdown of fish which are treated by 375 mg/kg vitamin E were respectively 0.92±0.05 mm, 8.86±4.62%, 56.00±29.18%, and 67.35±17.67% higher than control. Thus, 375 mg/kg of dietary vitamin E suplementating was a best dose to improve female broodstock productivity of the fantail goldfish

 

Keywords: female broodstock, fantail goldfish, vitamin E, reproduction quality

 

 

ABSTRAK

 

Penelitian dilakukan untuk menguji suplementasi vitamin E dengan dosis berbeda dicampur ke dalam pakan komersial terhadap produktivitas induk betina ikan komet Carassius auratus auratus. Penelitian ini menggunakan empat perlakuan dengan tiga ulangan. Dosis vitamin E yang diberikan, yaitu 0, 125, 250, dan 375 mg/kg pakan. Vitamin E dilarutkan dalam minyak nabati dan dicampur dengan putih telur sebagai perekat pada pakan. Vitamin E disemprotkan ke pakan dan dikeringanginkan. Induk betina ikan komet pascasalin dengan bobot 72,78±19,47 g diberi perlakuan selama 40 hari. Hasil penelitian menunjukkan bahwa induk betina ikan komet yang diberi suplementasi vitamin E sebanyak 375 mg/kg dalam pakan memiliki diameter telur 0,92±0,05 mm, gonadosomatic index 8,86±4,62%, fekunditas 56,00±29,18 butir/g ikan, dan germinal vesicle breakdown 67,35±17,67% yang lebih tinggi dibandingkan kontrol. Dengan demikian, suplementasi vitamin E sebesar 375 mg/kg pada pakan adalah dosis terbaik dalam meningkatkan produktivitas induk betina ikan komet.

 

Kata kunci: induk betina, ikan komet, vitamin E, kualitas reproduksi

 
Frequency and persistency of DNA vaccine encoding GP25 by oral on common carp
Authors: Nuryati Sri , Yuliyanti , , Alimuddin ,
Published: February 2017

ABSTRACT Koi herpesvirus (KHV) is a major viral pathogen that infects common carp and koi. KHV disease outbreak is happened in almost all centre of common carp culture in Indonesia and caused mass mortality. Deoxyribonucleic acid (DNA) vaccination method is one of ways to cope with KHV infection. Vaccines were commonly given by injection. The aim of this research was to get frequency and persistency of DNA vaccine encoding GP25 given by oral delivery method in common carp. This research would like to determine dose, frequency of vaccination, persistency of DNA vaccine and culture medium for the bacterial host. DNA vaccine persistency test was done by using polymerase chain reaction (PCR) method with the specific primer for GP25 gene. The results showed that level of DNA vaccine that could be detected in feed was 7.56 ng (equal to 1.598×1010 copies). Efficient culture medium for Escherichia coli DH5α carrying DNA vaccine was LB triptone. Feeding fish with diet supplemented with 1 mL E. coli DH5α containing DNA vaccine for each fish and two times a week allowed persistence of DNA vaccine in kindney and spleen. Keywords: common carp, KHV, DNA vaccine, GP25, persistance  ABSTRAK Koi herpesvirus (KHV) adalah virus patogen utama yang menginfeksi ikan mas dan ikan koi. Wabah penyakit KHV terjadi di hampir semua sentra budidaya ikan mas di Indonesia dan menyebabkan kematian massal ikan. Metode vaksinasi DNA merupakan salah satu cara yang dapat dilakukan untuk menanggulangi serangan KHV. Pemberian vaksin umumnya dilakukan dengan cara injeksi. Tujuan penelitian ini adalah untuk menguji frekuensi dan persistensi vaksin DNA GP25 antivirus KHV yang diberikan melalui oral pada ikan mas. Pada penelitian ini dilakukan uji dosis, frekuensi pemberian vaksin, persistensi vaksin DNA, dan media kultur bakteri inang. Persistensi vaksin DNA dianalisis menggunakan metode PCR dengan primer spesifik gen GP25. Hasil penelitian menunjukkan bahwa dosis vaksin DNA yang dapat terdeteksi dalam pakan adalah 7,56 ng (setara dengan 1,598×1010 copy). Media kultur yang efisien bagi bakteri Escherichia coli DH5α pembawa vaksin DNA adalah LB tripton. Pemberian pakan bervaksin berupa bakteri konsentrasi 1 mL/ekor ikan dengan frekuensi dua kali seminggu menghasilkan persistensi DNA GP25 di ginjal dan limpa. Kata kunci: ikan mas, KHV, vaksin DNA, GP25, persistensi
Feeding for larvae of catfish Pangasionodon sp. larvae in different ages
Authors: Suprayudi Muhammad Agus , Ramadhan Ricky , Jusadi Dedi
Published: February 2017

ABSTRACT Sludge worm (Tubifex sp.) as natural feed on catfish (Pangasionodon sp.) larvae rearing is available in limited amount especially during rainy season. It becomes a constraint factor for larvae rearing sector. This research was conducted to evaluate the appropriate initial age of catfish larvae to get artificial feed as sludge worm replacement. Evaluation was conducted on the growth and survival of catfish larvae in 14 days of culture. There were four treatments of feeding in triplicates i.e. larvae were given natural feed without artificial feed, given artificial feed started from d3, d6, and d9) with three replications. The results showed that larvae fed on artificial feed on d3 had the lowest growth compared to the other treatments, whereas the survival was not significantly different (P>0.05) among the treatments. As a conclusion, artificial feed could be used to replace natural feed for catfish larvae started at the age of nine days. Keywords: sludge worm, catfish larvae, artificial feed  ABSTRAK Cacing sutra (Tubifex sp.) tersedia dalam jumlah terbatas terutama pada musim penghujan sebagai pakan alami dalam usaha pembenihan ikan patin (Pangasionodon sp.). Ini menjadi kendala dalam usaha pembenihan. Penelitian ini dilakukan untuk mengevaluasi umur larva ikan patin yang tepat untuk mulai diberi pakan buatan menggantikan cacing sutra. Evaluasi dilakukan pada pertumbuhan dan kelangsungan hidup larva ikan patin umur 14 hari. Selama pemeliharaan, larva diberi pakan dengan empat perlakuan; pemberian pakan alami tanpa pakan buatan, pemberian pakan buatan mulai d3, d6, dan d9 dengan tiga ulangan untuk masing-masing perlakuan. Hasil penelitian menunjukkan bahwa perlakuan pemberian pakan buatan mulai d3 memiliki pertumbuhan panjang yang terkecil dibandingkan perlakuan lain, sedangkan tingkat kelangsungan hidup larva tidak berbeda nyata (P>0,05) antarperlakuan. Berdasarkan hasil penelitian, dapat disimpulkan bahwa pakan buatan dapat digunakan sebagai pengganti pakan alami mulai larva ikan patin umur sembilan hari. Kata kunci: cacing sutra, larva ikan patin, pakan buatan
Feeding rate of freshwater eel Anguilla bicolor bicolor: at the body weight of 1–2 g
Authors: Fekri Latifa , Affandi Ridwan , Budiardi Tatag
Published: February 2017

ABSTRACT The aim of the research was to determine feed requirement for the maintenance, optimum, and maximum growth of freshwater eel (Anguilla bicolor bicolor) seed (1–2 g body weight). Feed used in this research was KRA feed with 46% protein content, with different feeding level (FR) at 0%, 5%, 10%, and 15% of fish biomass. The experiment was conducted in 30 days. Parameters measured were survival and specific growth (SGR). The results showed that survival of fish in all treatments were 100%, except in fish fed on 15% of biomass (only 96). Fish growth with feeding of 0%, 5%,10%, and 15%, were -1.06%; 0.42%; 0.73%; and 0.19%, respectively. Based on the analysis of the relationship between feeding level and growth, the feed requirement for maintenance, optimum, and maximum growth in 1–2 g freshwater eel seed were 3.3%; 7.0%; and 9.5% of the biomass, respectively. Keywords: eel seed, feed requirement, growth ABSTRAK Penelitian dilakukan dengan tujuan untuk mendapatkan kebutuhan pakan untuk maintenance, dan untuk pertumbuhan optimum serta maksimum benih ikan sidat (Anguilla bicolor bicolor) (bobot tubuh 1–2 g). Pakan yang digunakan pada penelitian ini adalah pakan KRA dengan kadar protein 46%, dengan tingkat pemberian pakan 0%, 5%, 10%, dan 15% dari bobot biomassa ikan. Pemeliharaan berlangsung selama 30 hari. Parameter yang diukur meliputi sintasan (STS) dan laju pertumbuhan spesifik (LPS). Hasil penelitian menunjukkan bahwa STS benih ikan sidat selama pemeliharaan, memiliki nilai yang baik (100%) kecuali pada pemberian pakan 15% (hanya 96%). Laju pertumbuhan benih ikan sidat selama pemeliharaan dengan pemberian pakan 0%, 5%, 10%, dan 15% berturut-turut adalah -1,06%; 0,42%; 0,73%; dan 0,19%. Berdasarkan analisis hubungan antara tingkat pemberian pakan dan laju pertumbuhan spesifik, maka kebutuhan pakan maintenance, pertumbuhan optimum dan maksimum benih ikan sidat berukuran 1–2 g berturut-turut adalah 3,3%; 7%; dan 9,5% dari biomassa. Kata kunci: benih ikan sidat, kebutuhan pakan, pertumbuhan
Gracilaria spp. morphology cultured in brackish water pond Pantai Sederhana Village, Muara Gembong
Authors: Soelistyowati Dinar Tri , Murni Ida Ayu Amarilia Dewi , Wiyoto ,
Published: February 2017

ABSTRACT Gracilaria spp. is a euryhaline species of seaweed which can live in the marine and brackish water. Development of Gracilaria spp. culture in Bekasi is potential because this seaweed can be cultured in ex shrimp pond by polyculture system. The objective of this research was to evaluate the phenotype morphological characteristic of Gracilaria spp. based on and its relationship with shrimp pond water quality. Sampling was done at three shrimp ponds with a salinity range at 13.7–19.2 g/L. Phenotypical characteristics of Gracilaria spp. consisted of colour and thallus morfometrics, while measurement of water quality consisted of physical and chemical charactersof shrimp pond. The result showed that Gracilaria spp. generally had light brown colour. At salinity higher than 13.7 g/kg, the number of secondary thalli increased, the distance among internode tertiary thalli declined, and the number of ramification index increased. Salinity showed a positive correlation with remification index which was 0.571. Keywords: Gracilaria spp., remification index, phenotype, salinity, brackishwater culture  ABSTRAK Gracilaria spp. merupakan spesies rumput laut eurihalin yang dapat hidup di laut dan di perairan payau. Pengembangan budidaya Gracilaria spp. di Bekasi potensial dilakukan karena memanfaatkan tambak bekas budidaya udang dengan sistem polikultur. Penelitian ini bertujuan untuk mengevaluasi karakteristik fenotipe morfologi Gracilaria spp. dan hubungannya dengan kualitas air di tambak budidaya. Sampling dilakukan pada tiga tambak dengan kisaran salinitas 13,7–19,2 g/L. Karakterisasi fenotipe meliputi warna dan morfometrik talus Gracilaria spp., sedangkan parameter kualitas air meliputi karakter fisika dan kimia air tambak. Hasil menunjukkan talus Gracilarias spp. umumnya berwarna coklat muda dan pada salinitas di atas 13,7 g/L menunjukkan jumlah talus sekunder meningkat, jarak internode talus tersier menurun, dan indeks percabangan meningkat (P<0,05). Salinitas berkorelasi positif dengan indeks percabangan sebesar 0,571. Kata kunci: Gracilaria spp., indeks percabangan, fenotipe, salinitas, budidaya air payau
The growth of eel fed with different protein level and protein-energy ratio
Authors: Nawir Fitria , Utomo Nur Bambang Priyo , Budiardi Tatag
Published: February 2017

ABSTRACT The study was aimed to determine the optimum dietary protein level and energy protein ratio which can optimize growth performance of the eel A. bicolor bicolor on nursery phase.  Four treatments and three replications were applied in this study. The treatments were  P1, containing 37.66% protein with energy protein ratio 14.75 kcal GE/g (37.66%; 14.75 kcal GE/g), treatment P2 (41.30%; 13.51 kcal GE/g), treatment P3 (45.38%; 12.27 kcal GE/g), and treatment P4 (49.60%; 11.31 kcal GE/g). Eels used for this study were 6.5±0.3 g in average body weight. Eels were reared in a series of aquaria with dimension 90×40×40 cm3 and filled with 100 L of fresh water. Total weight of eel stocked in aquarium were 400 g. Eels were fed until satiated  twice a day at 8 am and 4 pm for 60 days. The result showed  that different protein level and energy protein ratio was significantly affected  growth performance (feed consumption, specific growth rate, feed efficiency, protein retention, and lipid retention), protein and fat of whole body eels at confident limit of 5%. In contrary, there was no significant different on the survival rate, hepatosomatic index, ash content, and nitrogen free extract of the body eel. The optimal growth performance was reached by dietary protein level and energy protein ratio of 45.38%; 12.27 kcal GE/g and 49.60%; 11.31 kcal GE/g. Keywords: Anguilla bicolor bicolor, energy protein ratio, feed, growth performance, protein  ABSTRAK Penelitian ini bertujuan menentukan kadar protein dan rasio energi protein optimum yang dapat meningkatkan kinerja pertumbuhan ikan sidat A. bicolor bicolor fase pendederan. Empat macam perlakuan dan tiga ulangan digunakan dalam penelitian ini. Perlakuan tersebut adalah P1 yang mengandung protein 37,66%; dengan rasio energi protein 14,75 kkal GE/g (37,66%; 14,75 kkal GE/g), perlakuan P2 (41,30%; 13,51 kkal GE/g), perlakuan P3 (45,38%; 12,27 kkal GE/g) dan perlakuan P4 (49,60%; 11,31 kkal GE/g). Bobot rata-rata ikan sidat yang digunakan adalah 6,5±0,3 g. Ikan sidat dipelihara dalam akuarium berukuran 90×40×40 cm3 dengan volume air 100 L. Total bobot ikan yang digunakan dalam setiap akuarium adalah 400 g. Ikan sidat diberi pakan sekenyangnya dengan frekuensi dua kali sehari yaitu pukul 08.00 dan pukul 16.00 WIB selama 60 hari. Hasil menunjukkan bahwa pemberian kadar protein dan rasio energi protein pakan berbeda, memberikan pengaruh nyata (P<0,05) terhadap kinerja pertumbuhan (jumlah konsumsi pakan, laju pertumbuhan spesifik, efisiensi pakan, retensi protein, retensi lemak), protein tubuh dan lemak tubuh, tetapi tidak berpengaruh nyata (P>0,05) terhadap tingkat kelangsungan hidup, indeks hepatosomatik, kadar abu, dan bahan ekstrak tanpa nitrogen (BETN) tubuh ikan sidat. Kinerja pertumbuhan optimal dicapai oleh kadar protein dan rasio energi protein pakan 45,38%; 12,27 kkal GE/g dan 49,60%; 11,31 kkal GE/g.  Kata kunci: Anguilla bicolor bicolor, kinerja pertumbuhan, pakan, protein, rasio energi protein
Application of different honey and dilution ratios on sperm quality of Pangasianodon hypopthalmus
Authors: Arfah Harton , Hasan Fahmi , Setiawati Mia
Published: February 2017

ABSTRACT The aim of this study was to determine the effect of various types of honey (longan honey, lychee honey, and cottonwoods honey) on sperm quality of Siamese catfish Pangasianodon hypopthalmus with different dilution ratios after the storage period which includes sperm viability, fertilization rate, and hatching rate. The best treatment was obtained on the aplication of longan honey as a sperm diluent at 1:50 dilution ratio, with sperm viability 96.33±0.58%, fertilization rate 97.10±0.70%, and hatching rate 93.44±2.39%. Keywords: Siamese catfish, Pangasianodon hypophthalmus, honey, sperm dilution ratio  ABSTRAK Tujuan penelitian ini adalah mengevaluasi pengaruh pemberian berbagai jenis madu (madu kelengkeng, madu leci, dan madu randu) terhadap kualitas sperma ikan patin siam Pangasianodon hypopthalmus dengan rasio pengenceran berbeda setelah masa penyimpanan, yang meliputi viabilitas sperma, tingkat fertilisasi, dan derajat penetasan. Hasil penelitian menunjukkan bahwa perlakuan terbaik didapatkan pada pemberian madu kelengkeng dengan rasio pengenceran 1:50 dengan viabilitas sperma 96,33±0,58%, tingkat fertilisasi 97,10±0,70%, dan derajat penetasan 93,44±2,39%. Kata kunci: ikan patin, Pangasianodon hypopthalmus, madu, rasio pengenceran sperma
Glycogen and proximate content of white shrimp fed on different carbohydrate level and feeding frequency
Authors: Zainuddin , , Haryati , , Aslamyah Siti
Published: February 2017

ABSTRACT White shrimp Litopenaeus vannamei is one of penaeid shrimp which can be cultured either in traditional and advanced technology scale. One problem that commonly faced by pond farmer is the high price of feed with high protein content. This research was to identify the optimal level of carbohydrate and feeding frequency on glycogen deposit and chemical composition of white shrimp juvenile. Research used factorial completely randomized design with two factors in triplicates. Treatments were factor A, carbohydrate level in feed respectively A1 (30%), A2 (37%), A3 (44%), A4 (50%), and factor B daily feeding frequency respectively B1 (twice), B2 (four times), and B3 (six times) respectively. White shrimp used had an initial average body weight of 0.3 g/shrimp. Feeding level was 10% of shrimp body weight. Results showed that both factors, carbohydrate level in feed, feeding frequency and their interactions were not significantly different on glycogen deposit of white shrimp juvenile. Analysis result on chemical compositions of white shrimp juvenile consisted of protein, lipid, nitrogen-free extract, crude fiber, ash, and energy were generally increased after treatments. Keywords: glycogen deposit, feeding frequency, chemical composition, carbohydrate level ABSTRAK Udang vaname Litopenaeus vannamei merupakan salah satu jenis udang penaeid yang dapat dibudidayakan baik dalam skala teknologi tradisional maupun skala teknologi maju. Salah satu masalah yang dihadapi para petani tambak adalah tingginya harga pakan yang disebabkan karena tingginya kandungan protein pakan. Penelitian ini bertujuan untuk menguji efek pemberian pakan dengan kadar karbohidrat dan frekuensi pemberian berbeda terhadap deposit glikogen dan komposisi kimia tubuh juvenil udang vanamei. Penelitian menggunakan desain rancangan acak lengkap pola faktorial dengan dua faktor dan setiap faktor diberi masing-masing tiga ulangan. Perlakuan yang diuji adalah faktor A, kadar karbohidrat pakan berturut-turut A1 (30%), A2 (37%), A3 (44%), A4 (50%), dan faktor B, frekuensi pemberian pakan dalam sehari berturut-turut B1 (dua kali), B2 (empat kali), dan B3 (enam kali). Udang vaname yang digunakan memiliki bobot rata-rata 0,3 g/individu. Dosis pakan ditetapkan sebesar 10% dari bobot tubuh udang dengan frekuensi pemberian pakan disesuaikan dengan perlakuan. Hasil penelitian menunjukkan bahwa faktor level karbohidrat pakan, frekuensi pemberian pakan dan kombinasi keduanya tidak berpengaruh signifikan terhadap deposit glikogen juvenil udang vaname.  Hasil analisis terhadap komposisi kimia tubuh juvenil udang vaname meliputi protein, lemak, bahan ekstrak tanpa nitrogen (BETN), serat kasar, kadar abu dan energi secara umum mengalami peningkatan setelah diberikan perlakuan dibandingkan pada awal penelitian. Kata kunci: deposit glikogen, frekuensi pemberian pakan, komposisi kimia, level karbohidrat
Evaluation of PMSG (Oodev®) application on hepatosomatic and gonadosomatic index of snakehead fish
Authors: Hutagalung Rizal Akbar , Widodo Maheno Sri , Faqih Abdul Rahem
Published: February 2017

ABSTRACT Snakehead fish (Channa striata) is a freshwater fish commodities that have high economic value, while their needs depend on natural catching. In general, hormonal induction commonly used for the efficiency of female parent utilization and enhancement quality and quantity of fish fry. One of the hormones that can be used is pregnant mare serum gonadotropin (PMSG) and antidopamine with trademark Oodev®. These hormones contain many elements follicle stimulating hormone (FSH) which can trigger the early stages of gonad maturity. The purpose of this research was to determine the effectiveness of hormone PMSG on gonadosomatic index (GSI) and hepatosomatic index (HSI). The size of female fish was 30−40 cm, weighing of 500−700 g, adapted in the aquarium and then induced with different doses of PMSG, namely: treatment A dose: 0.75 mL/kg; B: 1.0 mL/kg; C: 1.25 mL/kg; D: 1.5 mL/kg and be repeated three times. Fishes were then reared up to 72 hours to determine the development of GSI and HSI. The results showed that the best treatment was at dose of 1.25 mL/kg with GSI and HSI values of 1.37% and 3.35%, respectively. Keywords: PMSG, GSI, HSI, snakehead fish  ABSTRAK Ikan gabus (Channa striata) merupakan salah satu komoditas air tawar yang mempunyai nilai ekonomi tinggi. Saat ini pemenuhan kebutuhan hanya bergantung pada hasil penangkapan dari alam. Pada umumnya cara pemijahan buatan dengan induksi hormon dilakukan untuk efisiensi penggunaan induk serta peningkatan kualitas dan kuantitas benih ikan. Salah satu hormon yang dapat digunakan adalah pregnant mare serum gonadotropin (PMSG) dan antidopamin dengan merk dagang Oodev®. Hormon-hormon tersebut banyak mengandung unsur follicle stimulating hormon (FSH) yang dapat memicu kematangan gonad tahap awal. Tujuan dari penelitian ini adalah untuk menguji efektivitas hormon PMSG terhadap nilai indeks gonadosomatik (IGS) dan indeks hepatosomatik (IHS). Induk ikan gabus yang digunakan berukuran 30−40 cm dengan bobot 500–700 g, diaklimatisasikan di akuarium kemudian diinduksi hormon PMSG dengan perlakuan dosis A: 0,75 mL/kg; B: 1,0 mL/kg; C: 1,25 mL/kg; D: 1,5 mL/kg dan dilakukan pengulangan sebanyak tiga kali. Selanjutnya ditunggu hingga 72 jam untuk mengevaluasi perkembangan IGS dan IHS nya. Hasil penelitian menunjukkan bahwa perlakuan terbaik adalah dosis 1,25 ml/kg dengan nilai rata-rata IHS 1,37% dan IGS 3,35%, Kata kunci: PMSG, IGS, IHS, ikan gabus
Combination of Tubifex and artificial diet for catfish Pangasianodon hypophthalmus larvae
Authors: Jusadi Dedi , Anggraini Ria Septy , Suprayudi Muhammad Agus
Published: February 2017

ABSTRACT This research was conducted to evaluate the effect of different combination of feeding frequency of Tubifex (C) and artificial diet (P) on survival and growth rate of larval yellow tail catfish Pangasianodon hypophthalmus. Newly hatched larvae (d0) with total length of 0.44±0.04 cm were cultured for 14 days in 30×20×20 cm3 aquarium. Water volume in each aquarium was 9 L. At d5, larvae were fed on five different feeding frequencies either 6C+0PB, 5C+2PB, 3C+3PB, 2C+5PB, dan 0C+6PB. Feeding method used in this research was ad libitum. Results showed that survival and total length of d14 old larvae fed on 0C+6PB was lower than the groups fed on either 6C+0PB or three combinations of C+PB. Lipase activity tend to be increase in line with the increasing amount of artificial feed. On the other hand, larvae fed on 6C+0PB and 0C+6PB had the lowest protease activity. Therefore, larval catfish can be fed by combination of Tubifex and artificial feed. Keywords: artificial diet, lipase activity, protease activity, sludge worm, Pangasianodon hypophthalmus  ABSTRAK Penelitian ini mengevaluasi pengaruh perbedaan frekuensi pemberian pakan harian cacing sutra (C) dan pakan buatan (PB) terhadap sintasan dan pertumbuhan larva ikan patin Pangasianodon hypophthalmus. Larva ikan patin yang baru menetas berukuran 0,44±0,04 cm dipelihara dalam akuarium kaca berukuran 30×20×20 cm3 yang diisi air setinggi 15 cm selama 14 hari. Mulai dh5, larva diberi lima perlakuan frekuensi pemberian pakan, yaitu 6C+0PB, 5C+2PB, 3C+3PB, 2C+5PB, dan 0C+6PB. Pemberian pakan dilakukan secara ad libitum. Hasil penelitian menunjukkan bahwa larva yang diberi 6C+0PB dan kombinasi C+PB memiliki tingkat kelangsungan hidup dan panjang akhir yang tidak berbeda nyata. Namun larva yang diberi 0C+6PB, menghasilkan tingkat kelangsungan hidup dan panjang akhirnya terendah. Semakin banyak porsi sering frekuensi pemberian PB yang diberikan menunjukkan tren aktivitas lipase yang cenderung meningkat. Tetapi pada aktivitas protease pada pemberian 6C+0PB dan 0C+6PB lebih rendah dari ketiga perlakuan kombinasi C+PB. Dengan demikian, larva ikan patin dapat dibudidaya dengan menggunakan kombinasi cacing sutra dan pakan buatan. Kata kunci: pakan buatan, aktivitas lipase, aktivitas protease, cacing sutra, Pangasianodon hypophthalmus
Effect of electrical field on gonadal development of goldfish in saline media
Authors: Nirmala Kukuh , Habibie Ahmad , Arfah Harton
Published: February 2017

ABSTRACT The aim of this study was to evaluate the effect of electric field exposure duration at the voltage of 10 volt on goldfish Carassius auratus auratus gonadal development maintained in 3 ppt salinity media. The experiment consisted of four treatments in triplicates i.e. control, two, four, and six minutes of electrical-field exposure. The experiment design used was completely randomized design. Fish used was female goldfish at the density of 4 fish/aquarium with an average total length of 12.27±0.05 cm and average body weight of 22.29±0.54 g. Result of study showed that the electrical-field exposure at 10 volt for all duration treatments in 3 ppt of media salinity did not give significant effect on gonadosomatic index (GSI) and gonadal development of goldfish. Keywords: electrical field, Carassius auratus auratus, gonad, salinity  ABSTRAK Tujuan penelitian ini adalah untuk mengevaluasi pengaruh lama waktu pemaparan medan listrik dengan voltase 10 volt terhadap perkembangan gonad ikan komet Carassius auratus auratus yang dipelihara pada media bersalinitas 3 ppt. Perlakuan pada penelitian ini terdiri atas empat perlakuan, yaitu: perlakuan kontrol, dua, empat, dan enam menit. Rancangan percobaan yang digunakan adalah rancangan acak lengkap dengan empat perlakuan dan tiga ulangan. Ikan uji yang digunakan adalah ikan komet betina. Jumlah ikan yang digunakan adalah 4 ekor/akuarium dengan panjang total rata-rata 12,27±0,05 cm dan bobot tubuh rata-rata 22,29±0,54 g. Hasil penelitian menunjukkan bahwa pemberian medan listrik sebesar 10 volt dengan lama waktu pemaparan medan listrik pada semua perlakuan durasi di media pemeliharaan bersalinitas 3 ppt tidak memberikan pengaruh yang nyata terhadap parameter indeks gonadosomatik (GSI) dan perkembangan gonad ikan komet. Kata kunci: medan listrik, Carassius auratus auratus, gonad, salinitas
Rematuration Periods and Sperm Characteristics of Litopenaeus vannamei
Authors: Anwar L. Okmawati , Sumantadinata K. , Carman O.
Published: February 2017

The reproduction ability of male has an important role in seeds production of Litopenaeus vannamei. The objective of research was to know the sperm characteristics from each rematuration period. The research conducted in Broodstock and Nauply Quality Control Laboratory, Central Pertiwi Bahari, Lampung Province on April until July 2006. Five male shrimps obtained from High Health, Aquaculture Inc., Hawaii-USA, about 39.4 ± 1.51 g in weight and 16.4 ± 0.43 cm in length. Carefull stripping was applied to release the mature spermatophore from the thelicum. The results showed that number of sperm at the first to fourth rematuration period relatively constant (33.62x106 - 39.7402x106 cell). However the number of abnormal sperm were relatively increase slightly (1.2806x106 to 23.3576x106 cell) and also the number of death sperm (0.293x106 to 3.92x106 cell) while the number of normal sperm were relatively decrease (29.1158x106 to 23.3576x106 cell). There were no changes in head size and tail lenght of the sperm from each rematuration periods.

Keywords:  Litopenaeus vannamei, spermatozoa, rematuration

 

ABSTRAK

Kemampuan reproduksi udang jantan berperan penting dalam pembenihan udang vaname Litopenaeus vannamei. Penelitian ini bertujuan untuk mengetahui karakteristik sperma pada setiap periode rematurasi. Penelitian dilaksanakan di Laboratorium Kendali Mutu Induk dan Naupli, Central Pertiwi Bahari, Propinsi  Lampung, bulan April sampai Juli 2006. Udang jantan yang digunakan berasal dari High Health, Aquaculture Inc., Hawaii-USA, dengan ukuran berat 39,4 ± 1,51 g, panjang tubuh 16,4 ± 0,43 cm dan berjumlah lima ekor sebagai ulangan. Spermatofor dilepaskan dari telikum dengan teknik  pengurutan. Hasil penelitian menunjukkan jumlah sperma relatif stabil pada empat kali rematurasi (33,62x106 - 39,7402x106 sel). Jumlah sperma abnormal dan sperma mati berbanding terbalik dengan jumlah sperma normal. Jumlah sperma abnormal meningkat dari rematurasi pertama hingga rematurasi ke empat (1,2806x106 ke 23,3576x106 sel) demikian halnya jumlah sperma mati (0,293x106 ke 3,92x106 sel), sedangkan jumlah sperma normal mengalami penurunan (29,1158x106 ke 23,3576x106 sel). Tidak terdapat perubahan ukuran kepala dan panjang ekor sperma dalam setiap periode rematurasi.

Kata kunci :  Litopenaeus vannamei, spermatozoa, rematurasi