bluuebunny/crossref_metadata_2025 huggingface dataset:
Segmentation stability of human head and neck cancer medical images for radiotherapy applications under de-identification conditions: Benchmarking data sharing and artificial intelligence use-cases
Authors: Sahlsten Jaakko , Wahid Kareem A. , Glerean Enrico , Jaskari Joel , Naser Mohamed A. , He Renjie , Kann Benjamin H. , Mäkitie Antti , Fuller Clifton D. , Kaski Kimmo
Published: March 2023

Background

Demand for head and neck cancer (HNC) radiotherapy data in algorithmic development has prompted increased image dataset sharing. Medical images must comply with data protection requirements so that re-use is enabled without disclosing patient identifiers. Defacing, i.e., the removal of facial features from images, is often considered a reasonable compromise between data protection and re-usability for neuroimaging data. While defacing tools have been developed by the neuroimaging community, their acceptability for radiotherapy applications have not been explored. Therefore, this study systematically investigated the impact of available defacing algorithms on HNC organs at risk (OARs).

Methods

A publicly available dataset of magnetic resonance imaging scans for 55 HNC patients with eight segmented OARs (bilateral submandibular glands, parotid glands, level II neck lymph nodes, level III neck lymph nodes) was utilized. Eight publicly available defacing algorithms were investigated: afni_refacer, DeepDefacer, defacer, fsl_deface, mask_face, mri_deface, pydeface, and quickshear. Using a subset of scans where defacing succeeded (N=29), a 5-fold cross-validation 3D U-net based OAR auto-segmentation model was utilized to perform two main experiments: 1.) comparing original and defaced data for training when evaluated on original data; 2.) using original data for training and comparing the model evaluation on original and defaced data. Models were primarily assessed using the Dice similarity coefficient (DSC).

Results

Most defacing methods were unable to produce any usable images for evaluation, while mask_face, fsl_deface, and pydeface were unable to remove the face for 29%, 18%, and 24% of subjects, respectively. When using the original data for evaluation, the composite OAR DSC was statistically higher (p ≤ 0.05) for the model trained with the original data with a DSC of 0.760 compared to the mask_face, fsl_deface, and pydeface models with DSCs of 0.742, 0.736, and 0.449, respectively. Moreover, the model trained with original data had decreased performance (p ≤ 0.05) when evaluated on the defaced data with DSCs of 0.673, 0.693, and 0.406 for mask_face, fsl_deface, and pydeface, respectively.

Conclusion

Defacing algorithms may have a significant impact on HNC OAR auto-segmentation model training and testing. This work highlights the need for further development of HNC-specific image anonymization methods.
Bone marrow CD34+ molecular chimerism as an early predictor of relapse after allogeneic stem cell transplantation in patients with acute myeloid leukemia
Authors: Malagola Michele , Polverelli Nicola , Beghin Alessandra , Bolda Federica , Comini Marta , Farina Mirko , Morello Enrico , Radici Vera , Accorsi Buttini Eugenia , Bernardi Simona , Re Federica , Leoni Alessandro , Bonometti Davide , Brugnoni Duilio , Lanfranchi Arnalda , Russo Domenico
Published: March 2023

Background

Minimal residual disease (MRD) monitoring is an important tool to optimally address post-transplant management of acute myeloid leukemia (AML) patients.

Methods

We retrospectively analyzed the impact of bone marrow CD34+ molecular chimerism and WT1 on the outcome of a consecutive series of 168 AML patients submitted to allogeneic stem cell transplantation.

Results

The cumulative incidence of relapse (CIR) was significantly lower in patients with donor chimerism on CD34+ cells ≥ 97.5% and WT1 < 213 copies/ABL x 10^4 both at 1st month (p=0.008 and p<0.001) and at 3rd month (p<0.001 for both). By combining chimerism and WT1 at 3rd month, 13 patients with chimerism < 97.5% or WT1 > 213 showed intermediate prognosis. 12 of these patients fell in this category because of molecular chimerism < 97.5% at a time-point in which WT1 was < 213.

Conclusions

Our results confirm that lineage-specific molecular chimerism and WT1 after allo-SCT (1st and 3rd month) are useful MRD markers. When considered together at 3rd month, CD34+ molecular chimerism could represent an earlier predictor of relapse compared to WT1. Further studies are necessary to confirm this preliminary observation.
Identification of predictive factors for outcomes after robot-assisted partial nephrectomy based on three-dimensional reconstruction of preoperative enhanced computerized tomography
Authors: Li Qinyu , Zhang Yucong , Liu Man , Li Heng , Guan Wei , Meng Xiaoyan , Hu Zhiquan , Wang Zhihua , Wang Shaogang , Li Zhen , Liu Jihong , Liu Zheng
Published: March 2023

Background

Information from the RENAL score is limited. This study aimed to identify new parameters based on three-dimensional (3D) reconstruction of preoperative enhanced computerized tomography (CT) for predicting outcomes after robot-assisted partial nephrectomy (RPN).

Materials and methods

The records of kidney cancer patients who underwent RPN at Tongji Hospital from March 2015 to July 2019 were reviewed. Demographic data, laboratory examinations, postoperative hospitalization time, and enhanced CT were retrospectively collected. Some tumor parameters were obtained from 3D reconstruction of CT data. The association between these predictive factors and outcomes after RPN was analyzed.

Results

A larger tumor bed area (TBA) was associated with a longer warm ischemia time (WIT) (P-value <0.001) and tumor resection time (P-value <0.001). Moreover, TBA was significantly associated with the elevation of postoperative creatinine (P-value = 0.005). TBA (P = 0.008), distance from the tumor to the first bifurcation of the renal artery (DTA) (P <0.034), and RENAL score (P = 0.005) were significantly associated with WIT in univariate logistic regression. In multivariate logistic regression, TBA (P = 0.026) and DTA (P = 0.048) were independent risk factors for prolonged WIT (over 25 min). The predictive effect of the combination of TBA, DTA, and RENAL score was higher than the predictive effect of RENAL score alone for WIT (area under curve: 0.786 versus 0.72).

Conclusion

TBA and DTA are independently associated with the WIT of RPN, which provides additional assessment value for the complexity of kidney cancer in RPN over the RENAL score.
The significance of MUAC z-scores in diagnosing pediatric malnutrition: A scoping review with special emphasis on neurologically disabled children
Authors: Aydın Kürşad , Dalgıç Buket , Kansu Aydan , Özen Hasan , Selimoğlu Mukadder Ayşe , Tekgül Hasan , Ünay Bülent , Yüce Aysel
Published: March 2023

This review by a panel of pediatric gastroenterology-hepatology-nutrition and pediatric neurology experts aimed to address the significance of mid-upper arm circumference (MUAC) assessment in diagnosis of pediatric malnutrition. Specifically, the potential utility of recently developed MUAC z-score tape in clinical practice for larger patient populations was addressed including the neurologically disabled children. In accordance with the evidence-based data, four statements were identified by the participating experts on the utility of MUAC z-score tape, including (1) MUAC z-scores correlate with body mass index (BMI) and weight for height/length (WFH/l) z-scores in diagnosing malnutrition; (2) MUAC z-score tape offers a higher sensitivity to diagnose the mild and moderate malnutrition and better ability to track the changes in nutritional status over time than the other single datapoint measurements; (3) Using single-step MUAC z-score tape in children with cerebral palsy (CP) seems to provide more reliable data on anthropometry; and (4) The clinical value of the tool in classifying secondary malnutrition in CP should be investigated in large-scale populations. In conclusion, enabling single-step estimation of nutritional status in a large-scale pediatric population regardless of age and within a wide range of weight, without formal training or the need for ancillary reference charts and calculators, MUAC z-tape offers a favorable tool for easier and earlier diagnosis of pediatric malnutrition. Nonetheless, further implementation of MUAC z-score screening in larger-scale and/or special populations is necessary to justify its utility in relation to other primary anthropometric indicators in diagnosis of malnutrition as well as in treatment monitoring in the community and hospital setting.
Chemotherapy-induced cavitating Wilms' tumor pulmonary metastasis: Active disease or scarring? A case report and literature review
Authors: Zarfati Angelo , Martucci Cristina , Crocoli Alessandro , Serra Annalisa , Persano Giorgio , Inserra Alessandro
Published: March 2023

The second most common abdominal tumor in children is Wilms’ tumor, and the lung is where it most often metastasizes. The typical metastases are multiple, peripherally located, round, and variable-sized nodules. Atypical patterns are also possible and may create diagnostic challenges, especially in patients treated with chemotherapy. Among these, cavitating metastases are an anecdotal type of atypical secondary lung lesions. Here, we report a case of a chemotherapy-induced cavitating Wilms' tumor pulmonary metastasis discovered during the follow-up for an anaplastic nephroblastoma in a 6-year-old girl. Furthermore, we conducted a review of the existing literature on this exceedingly rare radiological pattern to establish its best management.
Risk factors for postoperative avascular necrosis of the femoral head in children with developmental dysplasia of the hip
Authors: Yong Ming , Xu Mengqiu , Lou Yue , Lin Gang
Published: March 2023

Aim

To investigate factors associated with postoperative avascular necrosis of the femoral head (ANFH) in developmental dysplasia of the hip (DDH) patients, and if or how the associations varied among different subpopulations of age, sex and surgical method.

Methods

Patients with DDH were enrolled between October 31, 2016 and July 15, 2020 in this retrospective cohort study. The average follow-up time was 21.42 ± 10.02 months. The outcome was postoperative ANFH. The main study variables were the DDH classification, Tonnis grade, International Hip Dysplasia Institute (IHDI) classification, and preoperative traction. Multivariate logistic regression was employed to assess the associations between main study variables and postoperative ANFH. Subgroup analysis was carried out based on age at reduction, sex and surgical method. Odds ratio (ORs) and 95% confidence intervals (CIs) were calculated.

Results

A total of 427 children with DDH were included, with 92 (21.55%) in the ANFH group, and 335 (78.45%) in the non-ANFH group. DDH classification was positively correlated with the risk of postoperative ANFH (OR = 4.14, 95% CI, 1.08–15.77, P = 0.038). Children with preoperative traction had a significantly decreased risk of postoperative ANFH in contrast to those without preoperative traction (OR = 0.37, 95% CI, 0.22–0.61, P < 0.001). Children aged 1–3 years who received preoperative traction has a significantly reduced risk of postoperative ANFH than those who did not receive preoperative traction (OR = 0.28, 95% CI, 0.15–0.51, P < 0.001). For children aged >3 years, positive association was found between DDH classification and the risk of postoperative ANFH (OR = 3.75, 95% CI, 1.51–9.31, P = 0.004). Girls with a more severe DDH type had a significantly higher risk of postoperative ANFH (OR = 3.80, 95% CI, 1.80–8.02, P < 0.001). Receiving preoperative traction was associated with a significantly decreased risk of postoperative ANFH in girls (OR = 0.37, 95% CI, 0.22–0.61, P < 0.001). For children undergoing open reduction, DDH classification was positively associated with the risk of postoperative ANFH (OR = 3.01, 95% CI, 1.65–5.50, P < 0.001), and those with preoperative traction had a lower risk of postoperative ANFH compared with those without preoperative traction (OR = 0.35, 95% CI, 0.20–0.61, P < 0.001).

Conclusion

DDH classification and preoperative traction were associated with the risk of postoperative ANFH, and these associations varied across DDH patients with different ages, sexes and surgical methods.
Staphylococcus aureus bacteremia complicated with non-traumatic mediastinal abscess in children: A case report
Authors: Li Yiyuan , Zhu Yu , Wan Chaomin , Wen Yang
Published: March 2023

Background

Staphylococcus aureus bacteremia complicated with non-traumatic mediastinal abscess rarely occurs in children. Herein, we report a case of S. aureus bacteremia in a previously healthy 15-month-old boy, which was complicated with a non-traumatic mediastinal abscess, followed by recovery without surgery

Case presentation

A previously healthy 15-month-old boy presented to the hospital with a high fever, accompanied by chills, lethargy, tachycardia, tachypnea, and slight cough. Contrast-enhanced computerized tomography revealed mediastinal abscess and blood culture analysis showed the presence of S. aureus which was methicillin-susceptible. With prompt initiation of antibiotic treatment, with appropriate duration, the patient successfully recovered without surgical drainage upon discharge.

Conclusions

Staphylococcus aureus bacteremia complicated with non-traumatic mediastinal abscess is rare in children, and early recognition and appropriate management are essential for a successful outcome.
Evaluation of therapeutic effects of tetramethylpyrazine nitrone in Alzheimer’s disease mouse model and proteomics analysis
Authors: Zhou Xinhua , Huang Kaipeng , Wang Yuqiang , Zhang Zaijun , Liu Yingying , Hou Qinghua , Yang Xifei , Hoi Maggie Pui Man
Published: March 2023

The pathophysiology of Alzheimer’s disease (AD) is multifactorial with characteristic extracellular accumulation of amyloid-beta (Aβ) and intraneuronal aggregation of hyperphosphorylated tau in the brain. Development of disease-modifying treatment for AD has been challenging. Recent studies suggest that deleterious alterations in neurovascular cells happens in parallel with Aβ accumulation, inducing tau pathology and necroptosis. Therefore, therapies targeting cellular Aβ and tau pathologies may provide a more effective strategy of disease intervention. Tetramethylpyrazine nitrone (TBN) is a nitrone derivative of tetramethylpyrazine, an active ingredient from Ligusticum wallichii Franchat (Chuanxiong). We previously showed that TBN is a potent scavenger of free radicals with multi-targeted neuroprotective effects in rat and monkey models of ischemic stroke. The present study aimed to investigate the anti-AD properties of TBN. We employed AD-related cellular model (N2a/APPswe) and transgenic mouse model (3×Tg-AD mouse) for mechanistic and behavioral studies. Our results showed that TBN markedly improved cognitive functions and reduced Aβ and hyperphosphorylated tau levels in mouse model. Further investigation of the underlying mechanisms revealed that TBN promoted non-amyloidogenic processing pathway of amyloid precursor protein (APP) in N2a/APPswe in vitro. Moreover, TBN preserved synapses from dendritic spine loss and upregulated synaptic protein expressions in 3×Tg-AD mice. Proteomic analysis of 3×Tg-AD mouse hippocampal and cortical tissues showed that TBN induced neuroprotective effects through modulating mitophagy, MAPK and mTOR pathways. In particular, TBN significantly upregulated PINK1, a key protein for mitochondrial homeostasis, implicating PINK1 as a potential therapeutic target for AD. In summary, TBN improved cognitive functions in AD-related mouse model, inhibited Aβ production and tau hyperphosphorylation, and rescued synaptic loss and neuronal damage. Multiple mechanisms underlie the anti-AD effects of TBN including the modulation of APP processing, mTOR signaling and PINK1-related mitophagy.
Polygoni Multiflori Radix interferes with bile acid metabolism homeostasis by inhibiting Fxr transcription, leading to cholestasis
Authors: Dai Yihang , Jia Zhixin , Fang Cong , Zhu Meixia , Yan Xiaoning , Zhang Yinhuan , Wu Hao , Feng Menghan , Liu Lirong , Huang Beibei , Li Yueting , Liu Jie , Xiao Hongbin
Published: March 2023

Objective: To explore the possible mechanisms of cholestasis induced by Polygoni Multiflori Radix (PM).

Methods: Low and high doses of water extract of PM were given to mice by gavage for 8 weeks. The serum biochemical indexes of aspartate aminotransferase (AST), alanine aminotransferase (ALT), glutamyltransferase (GGT) alkaline phosphatase (ALP) and so on were detected in the second, fourth, sixth, and eighth weeks after administration. At the end of the eighth week of administration, the bile acid metabolic profiles of liver and bile were screened by high-performance liquid chromatography tandem triple quadrupole mass spectrometry (HPLC-QQQ-MS/MS). Liver pathological changes were observed by hematoxylin and eosin staining. Real-time quantitative polymerase chain reaction (RT-qPCR) was used to detect the mRNA transcription of the target genes and Western blotting (WB) was used to the detect target protein expression.

Results: Biochemical tests results showed the values of ALP and GGT were two and three times greater than the normal values respectively, and the value of R was less than 2. Histopathology also showed that PM caused lymphocyte infiltration, a small amount of hepatocyte necrosis and nuclear fragmentation in mouse liver. The proliferation of bile duct epithelial cells was observed in the high group. These results indicated that PM may lead to cholestatic liver injury. HPLC-QQQ-MS/MS analysis with the multivariate statistical analysis revealed significant alterations of individual bile acids in liver and gallbladder as compared to those of the control group. RT-qPCR showed that the transcription of Fxr, Shp, Bsep, Bacs, Mdr2, and Ugt1a1 were downregulated and that of Cyp7a1, Mrp3, and Cyp3a11 was significantly upregulated in the treatment group. WB demonstrated that PM also markedly downregulated the protein expression of FXR, BSEP, and MDR2, and upregulated CYP7A1.

Conclusion: PM inhibited the expression of FXR, which reduced the expression of MDR2 and BSEP, leading to the obstruction of bile acids outflow, and increased the expression of CYP7A1, resulting in an increase of intrahepatic bile acid synthesis, which can lead to cholestasis.
Platycodin-D exerts its anti-cancer effect by promoting c-Myc protein ubiquitination and degradation in gastric cancer
Authors: Xu Qianqian , Pan Guangzhao , Wang Zhonglan , Wang Lingling , Tang Yancheng , Dong Jinyun , Qin Jiang-Jiang
Published: March 2023

Platycodin D (PD) is a triterpene saponin extracted from the root of Platycodon grandiflorum. It has been reported to exhibit multiple pharmacological and biological properties. There is substantial evidence to support that PD displays a wide range of anti-tumor activities. However, the detailed molecular mechanism still needs further elaboration. In the present study, to explore whether PD inhibits gastric cancer (GC) cell viability, eight GC cell lines and the GES-1 cell line (a gastric mucosal cell line) were tested. We found that PD exhibited better inhibitory activity on GC cell lines than on the non-tumor cell line. Besides, treatment with PD led to a significant cell cycle arrest, thereby causing subsequent apoptosis. Regarding the cell growth inhibition mechanism, PD can downregulate the protein level of c-Myc rather than its mRNA level in a dose-dependent manner. Further studies revealed that PD disturbed the overall ubiquitination level in GC cell lines and enhanced the ubiquitination-dependent degradation of c-Myc. Interestingly, the inhibition of cell viability by PD could be restored to a certain extent when the expression of c-Myc was recovered, suggesting that PD-mediated GC cell growth inhibition is closely associated with c-Myc expression. Our study proposes a novel molecular mechanism for PD inhibiting GC cell proliferation and growth by destabilizing the c-Myc protein. This work may lay a preliminary foundation for developing PD as an anti-cancer therapy.
Niclosamide does not modulate airway epithelial function through blocking of the calcium activated chloride channel, TMEM16A
Authors: Danahay Henry , Lilley Sarah , Adley Kathryn , Charlton Holly , Fox Roy , Gosling Martin
Published: March 2023

Niclosamide and benzbromarone have been described as inhibitors of the calcium activated chloride channel, TMEM16A, and on this basis have been considered and tested as clinical candidates for the treatment of airway diseases. However, both compounds have previously demonstrated activity on a range of additional biological targets and it is unclear from the literature to what extent any activity on TMEM16A may contribute to efficacy in these models of airway disease. The aim of the present study was therefore to examine the pharmacology and selectivity of these clinical candidates together with a structurally unrelated TMEM16A blocker, Ani9, in a range of functional assays to better appreciate the putative role of TMEM16A in the regulation of both epithelial ion transport and the development of an airway epithelial mucus secretory phenoptype. Benzbromarone and Ani9 both attenuated recombinant TMEM16A activity in patch clamp studies, whereas in contrast, niclosamide induced a paradoxical potentiation of the TMEM16A-mediated current. Niclosamide and benzbromarone were also demonstrated to attenuate receptor-dependent increases in intracellular Ca2+ levels ([Ca2+]i) which likely contributed to their concomitant attenuation of the Ca2+-stimulated short-circuit current responses of FRT-TMEM16A and primary human bronchial epithelial (HBE) cells. In contrast, Ani9 attenuated the Ca2+-stimulated short-circuit current responses of both cell systems without influencing [Ca2+]i which supports a true channel blocking mechanism for this compound. Additional studies using HBE cells revealed effects of both niclosamide and benzbromarone on global ion transport processes (absorptive and secretory) as well as signs of toxicity (elevated LDH levels, loss of transepithelial resistance) that were not shared by Ani9. Ani9 also failed to influence the IL-13 induced differentiation of HBE towards a goblet cell rich, mucus hypersecreting epithelium, whereas niclosamide and benzbromarone attenuated numbers of both goblet and multiciliated cells, that would be consistent with cellular toxicity. Together these data challenge the description of niclosamide as a TMEM16A blocker and illustrate a range of off-target effects of both niclosamide and benzbromarone which may contribute to the reported activity in models of airway function.
Access to medicines for rare diseases: A European regulatory roadmap for academia
Authors: Rosenberg Noa , van den Berg Sibren , Stolwijk Nina N. , Jacobs Bart A. W. , Post Hendrika C. , Pasmooij Anna M. G. , de Visser Saco J. , Hollak Carla E. M.
Published: March 2023

Background: Novel or repurposed medicines for rare diseases often emerge from fundamental research or empirical findings in academia. However, researchers may be insufficiently aware of the possibilities and requirements to bring novel medicinal treatment options to the patient. This paper aims to provide an easily applicable, comprehensive roadmap designed for academic researchers to make medicines for rare diseases available for patients by addressing the relevant regulatory frameworks, including marketing authorization and alternative routes.

Methods: Key points of the regulatory chapters “Placing on the Market” and “Scope” of Directive 2001/83/EC relating to medicinal products for human use were summarized. Provisions in EU directives regarding blood products, radiopharmaceuticals, and herbal and homeopathic medicinal products were excluded. Cross-referencing to other provisions was included. European case-law was retrieved from the InfoCuria database to exemplify the implications of alternative routes.

Results: Medicines may only be placed on the market with a valid marketing authorization. To obtain such authorization in Europe, a “Common Technical Document” comprising reports on quality and non-clinical and clinical studies must be submitted to a “competent authority”, a national medicine agency or the European Medicines Agency. Timely interaction of academic researchers with regulators via scientific advice may lead to better regulatory alignment and subsequently a higher chance for approval of academic inventions. Furthermore, reimbursement by national payers could be essential to ensure patient access. Apart from the marketing authorization route, we identified multiple alternative routes to provide (early) access. These include off-label use, named-patient basis, compassionate use, pharmacy compounding, and hospital exemption for Advanced Therapy Medicinal Products.

Discussion: Aligning academic (non-)clinical studies on rare diseases with regulatory and reimbursement requirements may facilitate fast and affordable access. Several alternative routes exist to provide (early) pharmaceutical care at a national level, but case-law demonstrates that alternative routes should be interpreted strictly and for exceptional situations only. Academics should be aware of these routes and their requirements to improve access to medicines for rare diseases.
Lie symmetry and exact homotopic solutions of a non-linear double-diffusion problem
Authors: Khan R. A. , Taj S. , Ahmed S. , Khan Ilyas , Eldin Sayed M.
Published: March 2023

The Lie symmetry method is applied, and exact homotopic solutions of a non-linear double-diffusion problem are obtained. Additionally, we derived Lie point symmetries and corresponding transformations for equations representing heat and mass transfer in a thin liquid film over an unsteady stretching surface, using MAPLE. We used these symmetries to construct new (Lie) similarity transformations that are different from those that are commonly used for flow and mass transfer problems. These new (Lie) similarity transformations map the partial differential equations of a mathematical model under consideration to ordinary differential equations along with boundary conditions. Lie similarity transformations are shown to lead to new solutions for the considered flow problem. These solutions are obtained using the homotopy analysis method to analytically solve the ordinary differential equations that resulted from the reduction of considered flow equations through Lie similarity transformations. With the aid of these solutions, effects of various parameters on the flow and heat transfer are discussed and presented graphically in this study.
The role and regulation mechanism of Chinese traditional fitness exercises on the bone and cartilage tissue in patients with osteoporosis: A narrative review
Authors: Sun Weibo , Zhang Xin-An , Wang Zhuo
Published: March 2023

Osteoporosis (ops) is a systemic degenerative bone disease characterized by bone mass reduction, bone mineral density loss, bone microstructure destruction, bone fragility, and increased fracture susceptibility. Thus far, drug therapy is the main method used to prevent and treat osteoporosis. However, long-term drug treatment will inevitably lead to drug resistance and certain side effects. In response, rehabilitation treatment is generally recommended, which involves drug supplementation combined with the treatment. A Chinese traditional fitness exercise is an organic combination of sports and traditional Chinese medicine with a series of advantages such as being safe, convenient, non-toxic, and harmless. Hence, it is one of the rehabilitation methods widely used in clinical practice. By searching the CNKI, PubMed, Web of Science, Embase, Cochrane Library, and other relevant databases, our research clarifies the current situation of four kinds of Chinese traditional fitness exercises widely used in clinical practice, namely, Taijiquan, Baduanjin, Wuqinxi, and Yijin Jing. In addition, the molecular mechanism of osteoporosis is summarized in this study. Based on the research, Chinese traditional fitness exercises are expected to directly stimulate the bone through a mechanical load to improve bone density. Moderate and regular traditional Chinese fitness exercises also improve osteoporosis by regulating the endocrine system with the secretion of hormones and factors such as estrogen and irisin, which are beneficial for bone formation. Finally, the purpose of promoting bone formation, reducing bone loss, and preventing and treating osteoporosis is achieved. The various means of Chinese traditional fitness exercises have different emphases, and the effect of improving bone density differs in various parts of the body. The exercisers may choose the exercise flexibly based on their own needs. Chinese traditional fitness exercises can improve the bone density of the exercisers and relieve pain, improve balance, and regulate the psychological state. Consequently, it is worth promoting to be applied in clinical practices.
A lightweight fetal distress-assisted diagnosis model based on a cross-channel interactive attention mechanism
Authors: Deng Yanjun , Zhang Yefei , Zhou Zhixin , Zhang Xianfei , Jiao Pengfei , Zhao Zhidong
Published: March 2023

Fetal distress is a symptom of fetal intrauterine hypoxia, which is seriously harmful to both the fetus and the pregnant woman. The current primary clinical tool for the assessment of fetal distress is Cardiotocography (CTG). Due to subjective variability, physicians often interpret CTG results inconsistently, hence the need to develop an auxiliary diagnostic system for fetal distress. Although the deep learning-based fetal distress-assisted diagnosis model has a high classification accuracy, the model not only has a large number of parameters but also requires a large number of computational resources, which is difficult to deploy to practical end-use scenarios. Therefore, this paper proposes a lightweight fetal distress-assisted diagnosis network, LW-FHRNet, based on a cross-channel interactive attention mechanism. The wavelet packet decomposition technique is used to convert the one-dimensional fetal heart rate (FHR) signal into a two-dimensional wavelet packet coefficient matrix map as the network input layer to fully obtain the feature information of the FHR signal. With ShuffleNet-v2 as the core, a local cross-channel interactive attention mechanism is introduced to enhance the model’s ability to extract features and achieve effective fusion of multichannel features without dimensionality reduction. In this paper, the publicly available database CTU-UHB is used for the network performance evaluation. LW-FHRNet achieves 95.24% accuracy, which meets or exceeds the classification results of deep learning-based models. Additionally, the number of model parameters is reduced many times compared with the deep learning model, and the size of the model parameters is only 0.33 M. The results show that the lightweight model proposed in this paper can effectively aid in fetal distress diagnosis.
Mechanisms of primordial follicle activation and new pregnancy opportunity for premature ovarian failure patients
Authors: Zhang Tuo , He Meina , Zhang Jingjing , Tong Yuntong , Chen Tengxiang , Wang Chao , Pan Wei , Xiao Ziwen
Published: March 2023

Primordial follicles are the starting point of follicular development and the basic functional unit of female reproduction. Primordial follicles are formed around birth, and most of the primordial follicles then enter a dormant state. Since primordial follicles are limited in number and can’t be renewed, dormant primordial follicles cannot be reversed once they enter the growing state. Thus, the orderly occurrence of primordial follicles selective activation directly affects the rate of follicle consumption and thus determines the length of female reproductive lifespan. Studies have found that appropriately inhibiting the activation rate of primordial follicles can effectively slow down the rate of follicle consumption, maintain fertility and delay ovarian aging. Based on the known mechanisms of primordial follicle activation, primordial follicle in vitro activation (IVA) technique has been clinically developed. IVA can help patients with premature ovarian failure, middle-aged infertile women, or infertile women due to gynecological surgery treatment to solve infertility problems. The study of the mechanism of selective activation of primordial follicles can contribute to the development of more efficient and safe IVA techniques. In this paper, recent mechanisms of primordial follicle activation and its clinical application are reviewed.
Oxygen uptake (V˙ O2) and pulmonary ventilation (V˙ E) during military surface fin swimming in a swimming flume: Effects of surface immersion
Authors: Castagna Olivier , Blatteau Jean-Eric , Druelle Arnaud , Amara Jordan , Lacour Jean-René
Published: March 2023

Introduction: During military fin swimming, we suspected that oxygen uptake (V˙ O2) and pulmonary ventilation (V˙ E) might be much higher than expected. In this framework, we compared these variables in the responses of trained military divers during land cycling and snorkeling exercises.

Methods: Eighteen male military divers (32.3 ± 4.2 years; 178.0 ± 5.0 cm; 76.4 ± 3.4 kg; 24.1 ± 2.1 kg m-2) participated in this study. They performed two test exercises on two separate days: a maximal incremental cycle test (land condition), and an incremental fin swimming (fin condition) in a motorized swimming flume.

Results: The respective fin and landV˙ O2max were 3,701 ± 39 mL min-1 and 4,029 ± 63 mL min-1 (p = 0.07), these values were strongly correlated (r2 = 0.78 p < 0.01). Differences in V˙ O2max between conditions increased relative to l;V˙ O2max (r2 = 0.4 p = 0.01). FinV˙ Emax values were significantly lower than landV˙ Emax values (p = 0.01). This result was related to both the significantly lower fin Vt and f (p < 0.01 and <0.04, respectively). Consequently, the finV˙ Emax/V˙ O2max ratios were significantly lower than the corresponding ratios for land values (p < 0.01), and the fin and landV˙ Emax were not correlated. Other parameters measured at exhaustion—PaO2, PaCO2, and SO2 - were similar in fin and land conditions. Furthermore, no significant differences between land and fin conditions were observed for peak values for heart rate, blood lactate concentration, and respiratory exchange ratio R.

Conclusion: Surface immersion did not significantly reduce the V˙ O2max in trained divers relative to land conditions. As long as V˙ O2 remained below V˙ O2max, the V˙ E values were identical in the two conditions. Only at V˙ O2max was V˙ E higher on land. Although reduced by immersion, V˙ Emax provided adequate pulmonary gas exchange during maximal fin swimming.
Genetic changes in the genus Vitis and the domestication of vine
Authors: Guzmán-Ardiles Ruth Elena , Pegoraro Camila , da Maia Luciano Carlos , Costa de Oliveira Antônio
Published: March 2023

The genusVitisbelongs to the Vitaceae family and is divided into two subgenera:MuscadiniaandVitis, the main difference between these subgenera being the number of chromosomes. There are many hypotheses about the origin of the genus, which have been formed with archaeological studies and lately with molecular analyses. Even though there is no consensus on the place of origin, these studies have shown that grapes have been used by man since ancient times, starting later on its domestication. Most studies point to the Near East and Greece as the beginning of domestication, current research suggests it took place in parallel in different sites, but in all casesVitis vinifera(L.) subsp.sylvestris[Vitis vinifera(L.) subsp.sylvestris(Gmelin) Hagi] seems to be the species chosen by our ancestors to give rise to the now knownVitis vinifera(L.) subsp.vinifera[=sativa (Hegi)= caucasica (Vavilov)]. Its evolution and expansion into other territories followed the formation of new empires and their expansion, and this is where the historical importance of this crop lies. In this process, plants with hermaphrodite flowers were preferentially selected, with firmer, sweeter, larger fruits of different colors, thus favoring the selection of genes associated with these traits, also resulting in a change in seed morphology. Currently, genetic improvement programs have made use of wild species for the introgression of disease resistance genes and tolerance to diverse soil and climate environments. In addition, the mapping of genes of interest, both linked to agronomic and fruit quality traits, has allowed the use of molecular markers for assisted selection. Information on the domestication process and genetic resources help to understand the gene pool available for the development of cultivars that respond to producer and consumer requirements.
Training set designs for prediction of yield and moisture of maize test cross hybrids with unreplicated trials
Authors: Terraillon Jérôme , Roeber Frank K. , Flachenecker Christian , Frisch Matthias
Published: March 2023

Unreplicated field trials and genomic prediction are both used to enhance the efficiency in early selection stages of a hybrid maize breeding program. No results are available on the optimal experimental design when combining both approaches. Our objectives were to investigate the effect of the training set design on the accuracy of genomic prediction in unreplicated maize test crosses. We carried out a cross validation study on basis of an experimental data set consisting of 1436 hybrids evaluated for yield and moisture for which genotyping information of 461 SNP markers were available. Training set designs of different size, implementing within environment prediction, within year prediction, across year prediction, and combinations of data sources across years and environments were compared with respect to their prediction accuracy. Across year prediction did not reach prediction accuracies that are useful for genomic selection. Within year prediction across environments provided useful correlations between observed and predicted breeding values. The prediction accuracies did not improve when adding to the training set data from previous years. We conclude that using all data available from unreplicated tests of the current breeding cycle provides a good accuracy of predicting test crosses, whereas adding data from previous breeding cycles, in which the genotypes are less related to the tested material, has only limited value for increasing the prediction accuracy.
Assessing farmland suitability for agricultural machinery in land consolidation schemes in hilly terrain in China: A machine learning approach
Authors: Yang Heng , Ma Wenqiu , Liu Tongxin , Li Wenqing
Published: March 2023

Identifying available farmland suitable for agricultural machinery is the most promising way of optimizing agricultural production and increasing agricultural mechanization. Farmland consolidation suitable for agricultural machinery (FCAM) is implemented as an effective tool for increasing sustainable production and mechanized agriculture. By using the machine learning approach, this study assesses the suitability of farmland for agricultural machinery in land consolidation schemes based on four parameters, i.e., natural resource endowment, accessibility of agricultural machinery, socioeconomic level, and ecological limitations. And based on “suitability” and “potential improvement in farmland productivity”, we classified land into four zones: the priority consolidation zone, the moderate consolidation zone, the comprehensive consolidation zone, and the reserve consolidation zone. The results showed that most of the farmland (76.41%) was either basically or moderately suitable for FCAM. Although slope was often an indicator that land was suitable for agricultural machinery, other factors, such as the inferior accessibility of tractor roads, continuous depopulation, and ecological fragility, contributed greatly to reducing the overall suitability of land for FCAM. Moreover, it was estimated that the potential productivity of farmland would be increased by 720.8 kg/ha if FCAM were implemented. Four zones constituted a useful basis for determining the implementation sequence and differentiating strategies for FCAM schemes. Consequently, this zoning has been an effective solution for implementing FCAM schemes. However, the successful implementation of FCAM schemes, and the achievement a modern and sustainable agriculture system, will require some additional strategies, such as strengthening farmland ecosystem protection and promoting R&D into agricultural machinery suitable for hilly terrain, as well as more financial support.
Zinc/iron-regulated transporter-like protein gene family in Theobroma cacao L: Characteristics, evolution, function and 3D structure analysis
Authors: Pacheco Daniel Dastan Rezabala , Santana Brenda Conceição Guimaraes , Pirovani Carlos Priminho , de Almeida Alex-Alan Furtado
Published: March 2023

The zinc/iron-regulated transporter-like protein (ZIP) gene family first identified in plants is highly distributed in the plant kingdom. This family has previously been reported to transport several essential and non-essential cationic elements, including those toxic to many economically important crops such as cacao (Theobroma cacao L.). In this article, we present a detailed study on physicochemical properties, evolution, duplication, gene structure, promoter region and TcZIP family three-dimensional protein structure. A total of 11 TcZIP genes have been identified to encode proteins from 309 to 435 aa, with localization in the plasma membrane and chloroplast, containing 6-9 putative domains (TM). Interspecies phylogenetic analysis subdivided the ZIP proteins into four groups. Segmental duplication events significantly contributed to the expansion of TcZIP genes. These genes underwent high pressure of purifying selection. The three-dimensional structure of the proteins showed that α helix conformations are predominant with several pocket sites, containing the metal binding site, with the residues leucine (LEU), alanine (ALA), glycine (GLY), serine (SER), lysine (LYS) and histidine (HIS) the most predicted. Regarding the analysis of the protein-protein interaction and enrichment of the gene ontology, four biological processes were assigned, the most important being the cation transport. These new discoveries expand the knowledge about the function, evolution, protein structures and interaction of ZIP family proteins in cacao and contribute to develop cacao genotypes enriched with important mineral nutrients as well as genotypes that bioaccumulate or exclude toxic metals.
Differentiate responses of soil nutrient levels and enzymatic activities to freeze-thawing cycles in different layers of moss-dominated biocrusts in a temperate desert
Authors: Zhang Qing , Li Jiwen , Zhang Shujun , Li Yonggang , Wu Nan , Zhou Xiaobing , Yin Benfeng , Zhang Yuanming
Published: March 2023

Introduction

The biological soil crust, a widespread phenomenon in arid and semi-arid regions, influences many ecological functions, such as soil stability, surface hydrology, and biogeochemical cycling. Global climate change has significantly altered winter and spring freeze-thaw cycles (FTCs) in mid and high-latitude deserts. However, it is unclear how these changes will affect the biological soil crust and its influence on nutrient cycling and soil enzyme activity.

Methods

We conducted this study in the Gurbantunggut Desert, a typical temperate desert, using the moss crust as an example of an evolved biological soil crust. Simulating the effects of different FTC frequencies (0, 5, and 15 times) on soil carbon, nitrogen, phosphorus-related nutrients, and extracellular enzyme activities allowed us to understand the relationship between soil environmental factors and nutrient multifunctionality during FTC changes.

Results

The results showed that recurrent FTCs significantly increased the accumulation of carbon and phosphorus nutrients in the soil and decreased the effectiveness of nitrogen nutrients. These changes gradually stabilized after 15 FTCs, with available nutrients showing greater sensitivity than the previous full nutrient level. FTCs inhibited carbon, nitrogen, and phosphorus cycle-related hydrolase activities and promoted carbon cycle-related oxidase activities in the crust layer. However, in the 0–3 cm layer, the carbon and phosphorus cycle-related hydrolase activities increased, while peroxidase and urease activities decreased. Overall, the nutrient contents and enzyme activities associated with the carbon, nitrogen, and phosphorus cycles were lower in the 0–3 cm layer than in the crust layer. In addition, the multifunctionality of nutrients in the soil decreased after 15 FTCs in the crust layer and increased after 5 FTCs in the 0–3 cm layer. Structural equation modeling showed that FTC, soil water content, pH, available nutrients, and extracellular enzyme activity had opposite effects on nutrient multifunctionality in different soil layers. The change in nutrient multifunctionality in the crust layer was primarily caused by changes in total nutrients, while soil water content played a greater role in the 0–3 cm layer. Regardless of the soil layer, the contribution of total nutrients was much higher than the contribution of available nutrients and extracellular enzyme activity. In conclusion, it is essential to consider different soil layers when studying the effects of global climate change on the nutrient cycling of the biological soil crust.
A Monte Carlo study of IRTree models’ ability to recover item parameters
Authors: Alarcon Gene M. , Lee Michael A. , Johnson Dexter
Published: March 2023

Item response tree (IRTree) models are theorized to extract response styles from self-report data by utilizing multidimensional item response theory (IRT) models based on theoretical decision processes. Despite the growing popularity of the IRTree framework, there has been little research that has systematically examined the ability of its most popular models to recover item parameters across sample size and test length. This Monte Carlo simulation study explored the ability of IRTree models to recover item parameters based on data created from the midpoint primary process model. Results indicate the IRTree model can adequately recover item parameters early in the decision process model, specifically the midpoint node. However, as the model progresses through the decision hierarchy, item parameters have increased associated error variance. The authors ultimately recommend caution when employing the IRTree framework.
Chinese students’ filial piety beliefs and procrastination in mathematics learning: The mediating role of academic emotions
Authors: Guo Meng , Cao Yiming , Hu Xiang
Published: March 2023

This study examined the relations between Chinese students’ filial piety beliefs and mathematics procrastination and the mediating role of academic emotions in the relations. Analysis of data on 1,476 primary school students in China with structural equation modeling revealed that students’ reciprocal and authoritarian filial piety beliefs were positively related to academic enjoyment and anxiety, respectively. Students’ procrastination in mathematics learning was positively related to anxiety and authoritarian filial piety beliefs and had negative associations with enjoyment and reciprocal filial piety beliefs. The bootstrap analysis results confirmed the mediating role of anxiety in the relation between authoritarian filial piety beliefs and procrastination. Reciprocal filial piety beliefs had negative indirect relationship with procrastination via enjoyment. The results were explained from a socio-cultural perspective. The theoretical contributions and practical implications are discussed.