bluuebunny/crossref_metadata_2025 huggingface dataset:
β-Mannanase Production Using Coffee Industry Waste for Application in Soluble Coffee Processing
Authors: Favaro Camila , Baraldi Ilton , Casciatori Fernanda , Farinas Cristiane
Published: February 2020

Soluble coffee offers the combined benefits of high added value and practicality for its consumers. The hydrolysis of coffee polysaccharides by the biochemical route, using enzymes, is an eco-friendly and sustainable way to improve the quality of this product, while contributing to the implementation of industrial processes that have lower energy requirements and can reduce environmental impacts. This work describes the production of hydrolytic enzymes by solid-state fermentation (SSF), cultivating filamentous fungi on waste from the coffee industry, followed by their application in the hydrolysis of waste coffee polysaccharides from soluble coffee processing. Different substrate compositions were studied, an ideal microorganism was selected, and the fermentation conditions were optimized. Cultivations for enzymes production were carried out in flasks and in a packed-bed bioreactor. Higher enzyme yield was achieved in the bioreactor, due to better aeration of the substrate. The best β-mannanase production results were found for a substrate composed of a mixture of coffee waste and wheat bran (1:1 w/w), using Aspergillus niger F12. The enzymatic extract proved to be very stable for 24 h, at 50 °C, and was able to hydrolyze a considerable amount of the carbohydrates in the coffee. The addition of a commercial cellulase cocktail to the crude extract increased the hydrolysis yield by 56%. The production of β-mannanase by SSF and its application in the hydrolysis of coffee polysaccharides showed promise for improving soluble coffee processing, offering an attractive way to assist in closing the loops in the coffee industry and creating a circular economy.
Chemical Composition of Essential Oils from Different Parts of Zingiber kerrii Craib and Their Antibacterial, Antioxidant, and Tyrosinase Inhibitory Activities
Authors: Pintatum Aknarin , Laphookhieo Surat , Logie Emilie , Berghe Wim Vanden , Maneerat Wisanu
Published: February 2020

The essential oils of the fresh rhizomes; flowers; and leaves of Zingiber kerrii Craib were investigated using different extraction techniques; including solid-phase microextraction (SPME), hydrodistillation (HD), and organic solvent (OS), and characterized by gas chromatography–mass spectrometry (GC–MS). A total of 37 SPME; 19 HD; and 36 OS compounds were identified from the rhizome extract of Z. kerrii; with the major components being α-pinene; β-pinene; and terpinen-4-ol; respectively. From the flower extract; 16 SPME; 2 HD; and 10 OS compounds were identified; (E)-caryophyllene was found as a major compound by these techniques. The leaf extract exhibited 20 SPME; 13 HD; and 14 OS compounds; with α-pinene; (E)-caryophyllene; and n-hexadecanoic acid being the major compounds; respectively. The rhizome extract showed tyrosinase inhibitory activity of 71.60% and a total phenolic content of 22.4 mg gallic acid/g. The IC50 values of the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) assays were 25.2 µg/mL and 153.6 µg/mL; respectively; and the ferric ion reducing antioxidant power (FRAP) assay value was 318.5 µM ascorbic acid equivalent (AAE)/g extract. The rhizome extract showed weak antibacterial activity. This extract showed no adverse toxicity in human keratinocyte (HaCaT) cell lines at concentrations below 200 µg/mL.
Role of Human Galectins in Inflammation and Cancers Associated with Endometriosis
Authors: Hisrich Brooke V. , Young R. Brant , Sansone Alison M. , Bowens Zachary , Green Lisa J. , Lessey Bruce A. , Blenda Anna V.
Published: February 2020

Galectins are a family of β-galactoside-binding proteins that contribute to multiple cellular functions, including immune surveillance and apoptosis. Human galectins are also important regulators of inflammation, making them a research target for various inflammatory diseases and tumorigenesis associated with pro-inflammatory conditions. This review focuses on the involvement of human galectins in modulation of inflammation and in the pathophysiology of endometriosis and endometriosis-associated neoplasms. Endometriosis is a chronic inflammatory disease with unknown etiology. Galectins-1, -3 and -9 were found to be overexpressed in ectopic and eutopic endometrium of females with endometriosis compared to those without endometriosis. These findings suggest galectins’ role in the progression on endometriotic lesions and their potential use as diagnostic biomarkers and/or targets for therapeutic approaches. Galectins-1, -3, and -9 have also been implicated in the development of endometriosis-associated neoplasms. Furthermore, galectin-3 has been shown to interact with KRAS protein and contribute to cellular growth, proliferation, inflammation, and the uptake of nutrients in endometriotic lesions and may be involved in the maintenance and propagation of endometriosis. These galectins have been shown to be upregulated in certain forms of cervical, ovarian, endometrial, and colon cancer associated with endometriosis and have become a potential target for anti-cancer therapies.
A Thermolabile Phospholipase B from Talaromyces marneffei GD-0079: Biochemical Characterization and Structure Dynamics Study
Authors: Durrani Rabia , Khan Faez Iqbal , Ali Shahid , Wang Yonghua , Yang Bo
Published: February 2020

Phospholipase B (EC 3.1.1.5) are a distinctive group of enzymes that catalyzes the hydrolysis of fatty acids esterified at the sn-1 and sn-2 positions forming free fatty acids and lysophospholipids. The structural information and catalytic mechanism of phospholipase B are still not clear. Herein, we reported a putative phospholipase B (TmPLB1) from Talaromyces marneffei GD-0079 synthesized by genome mining library. The gene (TmPlb1) was expressed and the TmPLB1 was purified using E. coli shuffle T7 expression system. The putative TmPLB1 was purified by affinity chromatography with a yield of 13.5%. The TmPLB1 showed optimum activity at 35 °C and pH 7.0. The TmPLB1 showed enzymatic activity using Lecithin (soybean > 98% pure), and the hydrolysis of TmPLB1 by 31P NMR showed phosphatidylcholine (PC) as a major phospholipid along with lyso-phospholipids (1-LPC and 2-LPC) and some minor phospholipids. The molecular modeling studies indicate that its active site pocket contains Ser125, Asp183 and His215 as the catalytic triad. The structure dynamics and simulations results explained the conformational changes associated with different environmental conditions. This is the first report on biochemical characterization and structure dynamics of TmPLB1 enzyme. The present study could be helpful to utilize TmPLB1 in food industry for the determination of food components containing phosphorus. Additionally, such enzyme could also be useful in Industry for the modifications of phospholipids.
Anticancer Activity of Rutin and Its Combination with Ionic Liquids on Renal Cells
Authors: Caparica Rita , Júlio Ana , Araújo Maria Eduarda Machado , Baby André Rolim , Fonte Pedro , Costa João Guilherme , Santos de Almeida Tânia
Published: February 2020

The renal cell carcinoma (RCC) is the most common type of kidney cancer. Identifying novel and more effective therapies, while minimizing toxicity, continues to be fundamental in curtailing RCC. Rutin, a bioflavonoid widely found in nature, has shown promising anticancer properties, but with limited applicability due to its poor water solubility and pharmacokinetics. Thus, the potential anticancer effects of rutin toward a human renal cancer cell line (786-O), while considering its safety in Vero kidney cells, was assessed, as well as the applicability of ionic liquids (ILs) to improve drug delivery. Rutin (up to 50 µM) did not show relevant cytotoxic effects in Vero cells. However, in 786-O cells, a significant decrease in cell viability was already observed at 50 µM. Moreover, exposure to rutin caused a significant increase in the sub-G1 population of 786-O cells, reinforcing the possible anticancer activity of this biomolecule. Two choline-amino acid ILs, at non-toxic concentrations, enhanced rutin’s solubility/loading while allowing the maintenance of rutin’s anticancer effects. Globally, our findings suggest that rutin may have a beneficial impact against RCC and that its combination with ILs ensures that this poorly soluble drug is successfully incorporated into ILs–nanoparticles hybrid systems, allowing controlled drug delivery.
Bioinformatics Analysis of Global Proteomic and Phosphoproteomic Data Sets Revealed Activation of NEK2 and AURKA in Cancers
Authors: Deb Barnali , Sengupta Pratyay , Sambath Janani , Kumar Prashant
Published: February 2020

Tumor heterogeneity attributes substantial challenges in determining the treatment regimen. Along with the conventional treatment, such as chemotherapy and radiotherapy, targeted therapy has greater impact in cancer management. Owing to the recent advancements in proteomics, we aimed to mine and re-interrogate the Clinical Proteomic Tumor Analysis Consortium (CPTAC) data sets which contain deep scale, mass spectrometry (MS)-based proteomic and phosphoproteomic data sets conducted on human tumor samples. Quantitative proteomic and phosphoproteomic data sets of tumor samples were explored and downloaded from the CPTAC database for six different cancers types (breast cancer, clear cell renal cell carcinoma (CCRCC), colon cancer, lung adenocarcinoma (LUAD), ovarian cancer, and uterine corpus endometrial carcinoma (UCEC)). We identified 880 phosphopeptide signatures for differentially regulated phosphorylation sites across five cancer types (breast cancer, colon cancer, LUAD, ovarian cancer, and UCEC). We identified the cell cycle to be aberrantly activated across these cancers. The correlation of proteomic and phosphoproteomic data sets identified changes in the phosphorylation of 12 kinases with unchanged expression levels. We further investigated phosphopeptide signature across five cancer types which led to the prediction of aurora kinase A (AURKA) and kinases-serine/threonine-protein kinase Nek2 (NEK2) as the most activated kinases targets. The drug designed for these kinases could be repurposed for treatment across cancer types.
Machilin D, a Lignin Derived from Saururus chinensis, Suppresses Breast Cancer Stem Cells and Inhibits NF-κB Signaling
Authors: Zhen Xing , Choi Hack Sun , Kim Ji-Hyang , Kim Su-Lim , Liu Ren , Yun Bong-Sik , Lee Dong-Sun
Published: February 2020

Cancer stem cells are responsible for breast cancer initiation, metastasis, and relapse. Targeting breast cancer stem cells (BCSCs) using phytochemicals is a good strategy for the treatment of cancer. A silica gel, a reversed-phase C18 column (ODS), a Sephadex LH-20 gel, thin layer chromatography, and high-performance liquid chromatography (HPLC) were used for compound isolation from Saururus chinensis extracts. The isolated compound was identified as machilin D by mass spectrometry and nuclear magnetic resonance (NMR). Machilin D inhibited the growth and mammosphere formation of breast cancer cells and inhibited tumor growth in a xenograft mouse model. Machilin D reduced the proportions of CD44+/CD24- and aldehyde dehydrogenase 1 (ALDH1)-positive cells. Furthermore, this compound reduced the nuclear localization of the NF-κB protein and decreased the IL-6 and IL-8 secretion in mammospheres. These results suggest that machilin D blocks IL-6 and IL-8 signaling and induces CSC death and thus may be a potential agent targeting BCSCs.
MicroRNAs as Theranostics Targets in Oral Carcinoma Stem Cells
Authors: Hsieh Pei-Ling , Liao Yi-Wen , Pichler Martin , Yu Cheng-Chia
Published: February 2020

Oral cancer belongs to head and neck squamous cell carcinoma and has been recognized as one of the most prevalent malignancies worldwide. Recent studies have suggested that cancer stem cells (CSCs) may participate in tumor initiation, metastasis and even recurrence, so the regulation of CSCs has drawn significant attention over the past decade. Among various molecules that are associated with CSCs, non-coding RNAs (ncRNAs) have been indicated as key players in the acquisition and maintenance of cancer stemness. In addition, accumulating studies have shown that the aberrant expression of these ncRNAs may serve as surrogate diagnostic markers or even therapeutic targets for cancer treatment. The current study reviews the previous work by us and others to summarize how these ncRNAs affect oral cancer stemness and their potential theranostic applications. A better understanding of the implication of these ncRNAs in oral tumorigenesis will facilitate the translation of basic ncRNA research into clinical application in the future.
Synergy between Non-Thermal Plasma with Radiation Therapy and Olaparib in a Panel of Breast Cancer Cell Lines
Authors: Lafontaine Julie , Boisvert Jean-Sébastien , Glory Audrey , Coulombe Sylvain , Wong Philip
Published: February 2020

Cancer therapy has evolved to a more targeted approach and often involves drug combinations to achieve better response rates. Non-thermal plasma (NTP), a technology rapidly expanding its application in the medical field, is a near room temperature ionized gas capable of producing reactive species, and can induce cancer cell death both in vitro and in vivo. Here, we used proliferation assay to characterize the plasma sensitivity of fourteen breast cancer cell lines. These assays showed that all tested cell lines were sensitive to NTP. In addition, a good correlation was found comparing cell sensitivity to NTP and radiation therapy (RT), where cells that were sensitive to RT were also sensitive to plasma. Moreover, in some breast cancer cell lines, NTP and RT have a synergistic effect. Adding a dose of PARP-inhibitor olaparib to NTP treatment always increases the efficacy of the treatment. Olaparib also exhibits a synergistic effect with NTP, especially in triple negative breast cancer cells. Results presented here help elucidate the position of plasma use as a potential breast cancer treatment.
A Review of ULK1-Mediated Autophagy in Drug Resistance of Cancer
Authors: Liu Li , Yan Lu , Liao Ning , Wu Wan-Qin , Shi Jun-Ling
Published: February 2020

The difficulty of early diagnosis and the development of drug resistance are two major barriers to the successful treatment of cancer. Autophagy plays a crucial role in several cellular functions, and its dysregulation is associated with both tumorigenesis and drug resistance. Unc-51-like kinase 1 (ULK1) is a serine/threonine kinase that participates in the initiation of autophagy. Many studies have indicated that compounds that directly or indirectly target ULK1 could be used for tumor therapy. However, reports of the therapeutic effects of these compounds have come to conflicting conclusions. In this work, we reviewed recent studies related to the effects of ULK1 on the regulation of autophagy and the development of drug resistance in cancers, with the aim of clarifying the mechanistic underpinnings of this therapeutic target.
Return to Work of Breast Cancer Survivors: Perspectives and Challenges for Occupational Physicians
Authors: Campagna Marcello , Loscerbo Roberto , Pilia Ilaria , Meloni Federico
Published: February 2020

Breast cancer is one of the most common diseases worldwide, mainly affecting the female gender. Considering the increase of breast cancer incidence and the decrease of mortality due to news diagnostic and therapeutic tools, the return to work issue after treatment is going to be very common in the next years. Occupational physicians therefore need to face the return to work and the fitness for work of workers previously diagnosed with breast cancer with a sufficient cultural and technical background. In addition to individual characteristics preceding the diagnosis, clinical outcome, lifestyles and occupational variables are the most impactful factors on return to work that need to be taken into account. The aim of this work is to analyze these factors and discuss the central role of occupational physicians in the decision-making process of returning to work in breast cancer survivors.
Visceral Adipose Tissue Radiodensity Is Linked to Prognosis in Hepatocellular Carcinoma Patients Treated with Selective Internal Radiation Therapy
Authors: Ebadi Maryam , Moctezuma-Velazquez Carlos , Meza-Junco Judith , Baracos Vickie E. , DunichandHoedl Abha R. , Ghosh Sunita , Sarlieve Philippe , Owen Richard J. , Kneteman Norman , Montano-Loza Aldo J.
Published: February 2020

Hepatocellular carcinoma (HCC) constitutes the fourth leading cause of cancer-related mortality. Various factors, such as tumor size, tumor multiplicity, and liver function, have been linked to the prognosis of HCC. The aim of this study was to explore the prognostic significance of muscle, subcutaneous and visceral adipose tissue (VAT) mass, and radiodensity, in a cohort of 101 HCC patients treated with selective internal radiation therapy (SIRT). Muscle and adipose tissue cross sectional area (cm2/m2) and radiodensity, reported as the Hounsfield Unit (HU), were determined using pre-SIRT computed tomography images. Cox proportional hazard models and exact logistic regression were conducted to assess associations between body composition and adverse outcomes. Majority of the patients were male (88%) with a mean VAT radiodensity of −85 ± 9 HU. VAT radiodensity was independently associated with mortality (HR 1.05; 95% CI: 1.01–1.08; p = 0.01), after adjusting for cirrhosis etiology, Barcelona Clinic Liver Cancer stage, previous HCC treatment, and portal hypertension markers. Patients with a high VAT radiodensity of ≥–85 HU had a two times higher risk of mortality (HR 2.01, 95% CI 1.14–3.54, p = 0.02), compared to their counterpart. Clinical features of portal hypertension were more prevalent in patients with high VAT radiodensity. High VAT radiodensity was associated with severe adverse events after adjusting for confounding factors. High VAT radiodensity is independently associated with both increased mortality and severe adverse events in patients treated with SIRT. VAT radiodensity measurement might serve as an objective approach to identify patients who will experience the most benefit from SIRT.
Effects of Germline CYP2W1*6 and CYP2B6*6 Single Nucleotide Polymorphisms on Mitotane Treatment in Adrenocortical Carcinoma: A Multicenter ENSAT Study
Authors: Altieri Barbara , Sbiera Silviu , Herterich Sabine , De Francia Silvia , Della Casa Silvia , Calabrese Anna , Pontecorvi Alfredo , Quinkler Marcus , Kienitz Tina , Mannelli Massimo , Canu Letizia , Angelousi Anna , Chortis Vasileios , Kroiss Matthias , Terzolo Massimo , Fassnacht Martin , Ronchi Cristina L.
Published: February 2020

Mitotane is the only approved drug for advanced adrenocortical carcinoma (ACC) and no biomarkers are available to predict attainment of therapeutic plasma concentrations and clinical response. Aim of the study was to evaluate the suitability of cytochrome P450(CYP)2W1 and CYP2B6 single nucleotide polymorphisms (SNPs) as biomarkers. A multicenter cohort study including 182 ACC patients (F/M = 121/61) treated with mitotane monotherapy after radical resection (group A, n = 103) or in not completely resectable, recurrent or advanced disease (group B, n = 79) was performed. CYP2W1*2, CYP2W1*6, CYP2B6*6 and CYP2B6 rs4803419 were genotyped in germline DNA. Mitotane blood levels were measured regularly. Response to therapy was evaluated as time to progression (TTP) and disease control rate (DCR). Among investigated SNPs, CYP2W1*6 and CYP2B6*6 correlated with mitotane treatment only in group B. Patients with CYP2W1*6 (n = 21) achieved less frequently therapeutic mitotane levels (>14 mg/L) than those with wild type (WT) allele (76.2% vs 51.7%, p = 0.051) and experienced shorter TTP (HR = 2.10, p = 0.019) and lower DCR (chi-square = 6.948, p = 0.008). By contrast, 55% of patients with CYP2B6*6 vs. 28.2% WT (p = 0.016) achieved therapeutic range. Combined, a higher rate of patients with CYP2W1*6WT+CYP2B6*6 (60.6%) achieved mitotane therapeutic range (p = 0.034). In not completely resectable, recurrent or advanced ACC, CYP2W1*6 SNP was associated with a reduced probability to reach mitotane therapeutic range and lower response rates, whereas CYP2B6*6 correlated with higher mitotane levels. The association of these SNPs may predict individual response to mitotane.
Proton Magnetic Resonance Spectroscopy Characterization of Rathke’s Cleft Cysts (RCCs): Relevance to the Differential Diagnosis of Pituitary Adenomas and RCCs
Authors: Ijare Omkar B. , Sharpe Martyn A. , Baskin David S. , Pichumani Kumar
Published: February 2020

Background: Rathke’s Cleft Cysts (RCCs) are rare epithelial cysts arising from remnants of the Rathke pouch in the pituitary gland. A subset of these lesions enlarge and produce a mass effect with consequent hypopituitarism, and may result in visual loss. Moreover, some RCCs with a high intra-cystic protein content may mimic cystic pituitary adenoma, which makes their differential diagnosis ambiguous. Currently, medical professionals have no definitive way to distinguish RCCs from pituitary adenomas. Therefore, preoperative confirmation of RCCs would be of help to medical professionals for the management and proper surgical decision making. The goal of this study is to identify molecular markers in RCCs. Methods: We characterized aqueous and chloroform extracts of surgically resected RCCs and pituitary adenomas using ex vivo 1H NMR spectroscopy. Results: All RCCs exclusively showed the presence of mucopolysaccharides which are glycosaminoglycans (GAGs) made up of disaccharides of aminosugars and uronic sugars. Conclusion: GAGs can be used as metabolite marker for the detection of RCCs and this knowledge will lay the groundwork for the development of a non-invasive, in vivo magnetic resonance spectroscopy methodology for the differential diagnosis of RCCs and pituitary adenomas using clinical MRI scanners.
Integrin α7 and Extracellular Matrix Laminin 211 Interaction Promotes Proliferation of Acute Myeloid Leukemia Cells and Is Associated with Granulocytic Sarcoma
Authors: Kobayashi Nobuhiko , Oda Tsukasa , Takizawa Makiko , Ishizaki Takuma , Tsukamoto Norifumi , Yokohama Akihiko , Takei Hisashi , Saitoh Takayuki , Shimizu Hiroaki , Honma Kazuki , Kimura-Masuda Kei , Kuroda Yuko , Ishihara Rei , Murakami Yuki , Murakami Hirokazu , Handa Hiroshi
Published: February 2020

Acute myeloid leukemia (AML) with granulocytic sarcoma (GS) is characterized by poor prognosis; however, its underlying mechanism is unclear. Bone marrow samples from 64 AML patients (9 with GS and 55 without GS) together with AML cell lines PL21, THP1, HL60, Kasumi-1, and KG-1 were used to elucidate the pathology of AML with GS. RNA-Seq analyses were performed on samples from seven AML patients with or without GS. Gene set enrichment analyses revealed significantly upregulated candidates on the cell surface of the GS group. Expression of the adhesion integrin α7 (ITGA7) was significantly higher in the GS group, as seen by RT-qPCR (p = 0.00188) and immunohistochemistry of bone marrow formalin-fixed, paraffin-embedded (FFPE) specimens. Flow cytometry revealed enhanced proliferation of PL21 and THP1 cells containing surface ITGA7 in the presence of laminin 211 and stimulated ERK phosphorylation; this effect was abrogated following ITGA7 knockdown or ERK inhibition. Overall, high ITGA7 expression was associated with poor patient survival (p = 0.0477). In summary, ITGA7 is highly expressed in AML with GS, and its ligand (laminin 211) stimulates cell proliferation through ERK signaling. This is the first study demonstrating the role of integrin α7 and extracellular matrix interactions in AML cell proliferation and extramedullary disease development.
Pyrolysis Products Distribution of Enzymatic Hydrolysis Lignin with/without Steam Explosion Treatment by Py-GC/MS
Authors: Dong Yuguo , Lu Xinyu , Hu Chengjuan , Li Liang , Hu Qixiang , Wang Dandan , Xu Chaozhong , Gu Xiaoli
Published: February 2020

This paper investigated the pyrolytic behaviors of enzymatic hydrolysis lignin (EHL) and EHL treated with steam explosion (EHL-SE) by pyrolysis-gas chromatography/mass spectrometer (Py-GC/MS). It was shown that the main component of the pyrolysis products was phenolic compounds, including G-type, H-type, S-type, and C-type phenols. With different treatment methods, the proportion of units in phenolic products had changed significantly. Meanwhile, proximate, elemental, and FTIR analysis of both lignin substrates were also carried out for a further understanding of the lignin structure and composition with or without steam explosion treatment. FTIR result showed that, after steam explosion treatment, the fundamental structural framework of the lignin substrate was almost unchangeable, but the content of lignin constituent units, e.g., hydroxyl group and alkyl group, evidently changed. It was noticeable that 2-methoxy-4-vinylphenol with 11% relative content was the most predominant pyrolytic product for lignin after steam explosion treatment. Combined with the above analysis, the structural change and pyrolysis product distribution of EHL with or without steam explosion treatment could be better understood, providing more support for the multi-functional utilization of lignin.
Highly Crystallized Pd/Cu Nanoparticles on Activated Carbon: An Efficient Heterogeneous Catalyst for Sonogashira Cross-Coupling Reaction
Authors: Wei Zhen , Xie Zunyuan , Gao Lingxiang , Wang Yanyan , Sun Huaming , Jian Yajun , Zhang Guofang , Xu Liwen , Yang Jianming , Zhang Weiqiang , Gao Ziwei
Published: February 2020

In the quest for efficient and recyclable heterogeneous catalysts for Sonogashira coupling reactions, a PEG-OMe (Methoxy Polyene Glycol) - mediated method was developed to immobilize Pd/Cu bimetallic nanoparticles on activated carbon (AC). Catalytic experiments showed that Pd/Cu@AC prepared in a PEG-OMe 500 had the highest activity. The morphology and composition of the catalyst were determined, and the identified crystallized and heterometallic nanoparticles of Pd/Cu were essential for an efficient catalytic cycle. The Pd/Cu@AC catalyst was successfully used in Sonogashira reactions (21 examples) including with aryl bromides as the coupling partner in EtOH at 80–100 °C. The catalyst was recycled at least nine times.
PPAR-Mediated Toxicology and Applied Pharmacology
Authors: Xi Yue , Zhang Yunhui , Zhu Sirui , Luo Yuping , Xu Pengfei , Huang Zhiying
Published: February 2020

Peroxisome proliferator-activated receptors (PPARs), members of the nuclear hormone receptor family, attract wide attention as promising therapeutic targets for the treatment of multiple diseases, and their target selective ligands were also intensively developed for pharmacological agents such as the approved drugs fibrates and thiazolidinediones (TZDs). Despite their potent pharmacological activities, PPARs are reported to be involved in agent- and pollutant-induced multiple organ toxicity or protective effects against toxicity. A better understanding of the protective and the detrimental role of PPARs will help to preserve efficacy of the PPAR modulators but diminish adverse effects. The present review summarizes and critiques current findings related to PPAR-mediated types of toxicity and protective effects against toxicity for a systematic understanding of PPARs in toxicology and applied pharmacology.
PVPred-SCM: Improved Prediction and Analysis of Phage Virion Proteins Using a Scoring Card Method
Authors: Charoenkwan Phasit , Kanthawong Sakawrat , Schaduangrat Nalini , Yana Janchai , Shoombuatong Watshara
Published: February 2020

Although, existing methods have been successful in predicting phage (or bacteriophage) virion proteins (PVPs) using various types of protein features and complex classifiers, such as support vector machine and naïve Bayes, these two methods do not allow interpretability. However, the characterization and analysis of PVPs might be of great significance to understanding the molecular mechanisms of bacteriophage genetics and the development of antibacterial drugs. Hence, we herein proposed a novel method (PVPred-SCM) based on the scoring card method (SCM) in conjunction with dipeptide composition to identify and characterize PVPs. In PVPred-SCM, the propensity scores of 400 dipeptides were calculated using the statistical discrimination approach. Rigorous independent validation test showed that PVPred-SCM utilizing only dipeptide composition yielded an accuracy of 77.56%, indicating that PVPred-SCM performed well relative to the state-of-the-art method utilizing a number of protein features. Furthermore, the propensity scores of dipeptides were used to provide insights into the biochemical and biophysical properties of PVPs. Upon comparison, it was found that PVPred-SCM was superior to the existing methods considering its simplicity, interpretability, and implementation. Finally, in an effort to facilitate high-throughput prediction of PVPs, we provided a user-friendly web-server for identifying the likelihood of whether or not these sequences are PVPs. It is anticipated that PVPred-SCM will become a useful tool or at least a complementary existing method for predicting and analyzing PVPs.
N6-Adenosine Methylation in RNA and a Reduced m3G/TMG Level in Non-Coding RNAs Appear at Microirradiation-Induced DNA Lesions
Authors: Svobodová Kovaříková Alena , Stixová Lenka , Kovařík Aleš , Komůrková Denisa , Legartová Soňa , Fagherazzi Paolo , Bártová Eva
Published: February 2020

The DNA damage response is mediated by both DNA repair proteins and epigenetic markers. Here, we observe that N6-methyladenosine (m6A), a mark of the epitranscriptome, was common in RNAs accumulated at UV-damaged chromatin; however, inhibitors of RNA polymerases I and II did not affect the m6A RNA level at the irradiated genomic regions. After genome injury, m6A RNAs either diffused to the damaged chromatin or appeared at the lesions enzymatically. DNA damage did not change the levels of METTL3 and METTL14 methyltransferases. In a subset of irradiated cells, only the METTL16 enzyme, responsible for m6A in non-coding RNAs as well as for splicing regulation, was recruited to microirradiated sites. Importantly, the levels of the studied splicing factors were not changed by UVA light. Overall, if the appearance of m6A RNAs at DNA lesions is regulated enzymatically, this process must be mediated via the coregulatory function of METTL-like enzymes. This event is additionally accompanied by radiation-induced depletion of 2,2,7-methylguanosine (m3G/TMG) in RNA. Moreover, UV-irradiation also decreases the global cellular level of N1-methyladenosine (m1A) in RNAs. Based on these results, we prefer a model in which m6A RNAs rapidly respond to radiation-induced stress and diffuse to the damaged sites. The level of both (m1A) RNAs and m3G/TMG in RNAs is reduced as a consequence of DNA damage, recognized by the nucleotide excision repair mechanism.
Fibroblasts from the Human Skin Dermo-Hypodermal Junction are Distinct from Dermal Papillary and Reticular Fibroblasts and from Mesenchymal Stem Cells and Exhibit a Specific Molecular Profile Related to Extracellular Matrix Organization and Modeling
Authors: Haydont Valérie , Neiveyans Véronique , Perez Philippe , Busson Élodie , Lataillade Jean-Jacques , Asselineau Daniel , Fortunel Nicolas O.
Published: February 2020

Human skin dermis contains fibroblast subpopulations in which characterization is crucial due to their roles in extracellular matrix (ECM) biology. This study investigates the properties of fibroblasts localized at the frontier of deep dermis and hypodermis, i.e., dermo-hypodermal junction fibroblasts (F-DHJ), which were compared to intermediate reticular dermis (Fr) and superficial papillary dermis (Fp) fibroblasts. F-DHJ differed from Fr and Fp cells in their wider potential for differentiation into mesodermal lineages and in their absence of contractility when integrated in a three-dimensional dermal equivalent. The transcriptomic profile of F-DHJ exhibited specificities in the expression of genes involved in ECM synthesis-processing and “tissue skeleton” organization. In accordance with transcriptome data, ECM proteins, notably Tenascin C, distributions differed between the reticular dermis and the dermo-hypodermal junction areas, which was documented in normal adult skin. Finally, genome-wide transcriptome profiling was used to evaluate the molecular proximity of F-DHJ with the two dermal fibroblast populations (Fp and Fr) and with the mesenchymal stem cells (MSCs) corresponding to five tissue origins (bone marrow, fat, amnion, chorion, and cord). This comparative analysis classified the three skin fibroblast types, including F-DHJ, as a clearly distinct group from the five MSC sample origins.
Electrochemical Behavior and Detection of Diclofenac at a Microporous Si3N4 Membrane Modified Water–1,6-dichlorohexane Interface System
Authors: Almbrok Eissa Mohamed , Yusof Nor Azah , Abdullah Jaafar , Zawawi Ruzniza Mohd
Published: February 2020

The electrochemical behavior when the liquid–liquid interface was modified by commercially available, microporous silicon nitride membrane, was achieved using cyclic voltammetry with tetramethyl ammonium. The transfer characteristics of the ionizable drug diclofenac ( DCF − ), as an anti-inflammatory, anti-rheumatic, antipyretic, and analgesic treatment in common use in biomedical applications, were also investigated across microporous silicon nitride-modified liquid interface. Thus, some thermodynamic variables for DCF − , such as the standard Gibbs energy of transfer, the standard transfer potential and lipophilicity were estimated. Furthermore, the influence of possible interfering substances (ascorbic acid, sugar, amino acid, urea, and metal ions) on the detection of DCF − was investigated. An electrochemical DCF sensor is investigated using differential pulse voltammetry (DPV) as the quantification technique, a linear range of 8–56 µM and a limit of detection of 1.5 µM was possible due to the miniaturized interfaces formed within silicon nitride.
Climate Change and Sustaining Heritage Resources: A Framework for Boosting Cultural and Natural Heritage Conservation in Central Italy
Authors: Shirvani Dastgerdi Ahmadreza , Sargolini Massimo , Broussard Allred Shorna , Chatrchyan Allison , De Luca Giuseppe
Published: February 2020

Climate change has dramatically affected the rainfall patterns and water systems in Central Italy. The vulnerability of this area to climate change and natural hazards necessitates that appropriate adaptation policies be put in place to protect heritage sites. This study aims to develop a cultural and natural heritage conservation framework for Central Italy that enhances the capacity of climate change adaptation for heritage resources. For this purpose, a comparison was made between the UNESCO (United National Educational, Scientific and Cultural Organization) Convention of 1972 and the European Landscape Convention of the Council of Europe to achieve a coherent vision for the protection of heritage resources in Europe. After describing the impacts of climate change on heritage resources in Central Italy, we analyze and suggest improvements to the conservation framework for wisely protecting heritage resources in a changing climate. The findings reveal that conservation sectors require assessments of the value of heritage resources at the territorial scale to effectively define conservation priorities, assess the vulnerabilities, and more precisely direct funding. In this respect, the integration of the European Landscape Convention with territorial planning may boost the unity of a conservation framework in terms of climate change while providing new opportunities for conservation authorities to develop adaptation policies.
Foam Formation and Interaction with Porous Media
Authors: Johnson Phillip , Vaccaro Mauro , Starov Victor , Trybala Anna
Published: February 2020

Foams are a common occurrence in many industries and many of these applications require the foam to interact with porous materials. For the first time interaction of foams with porous media has been investigated both experimentally and theoretically by O. Arjmandi-Tash et al. It was found that there are three different regimes of the drainage process for foams in contact with porous media: rapid, intermediate and slow imbibition. Foam formation using soft porous media has only been investigated recently, the foam was made using a compression device with soft porous media containing surfactant solution. During the investigation, it was found that the maximum amount of foam is produced when the concentration of the foaming agent (dishwashing surfactant) is in the range of 60–80% m/m. The amount of foam produced was independent of the pore size of the media in the investigated range of pore sizes. This study is expanded using sodium dodecyl sulphate (SDS), which has the same critical micelle concentration as the commercial dishwashing surfactant, where the foam is formed using the same porous media and compression device. During the investigation, it was found that 10 times the critical micelle concentration (CMC) is the optimum concentration for a pure SDS surfactant solution to create foam. Any further increase in concentration after that point resulted in no further mass of foam being generated.
Safety and Design Aspects of Powered Toothbrush—A Narrative Review
Authors: Ng Clarence , Tsoi James Kit Hon , Lo Edward C. M. , Matinlinna Jukka P.
Published: February 2020

The powered toothbrush has become a modern dental tool that is available in the supermarket. Indeed, the design of the powered toothbrush, e.g., mechanical and electrical, would affect not only the efficacy but also the safety of the products. This narrative review attempted to view the powered toothbrush from design, safety, and application points with respect to tufts, filaments, handles, mechanics, motions, and materials interactions from various available sources. Different brands and models of powered toothbrushes have their own designs that might affect the clinical outcome. The rotational design was advocated to be clinically more effective than the manual one, some modern models might be designed with vibrational or oscillation (or mixed) tufts head that might be useful in patients with specific needs, such as having xerostomia or for the elderly. To conclude, tuft retention design is important in the powered toothbrush as it contributes significantly to safety as the fallen off tufts, filaments and metal parts might cause injury. Tests revealing the retention force of brush head plates and brush head bristles will be significant references for consumers to determine which design of powered toothbrushes is relatively safer.
Correction: Ardia, D., et al. Return and Risk of Pairs Trading Using a Simulation-Based Bayesian Procedure for Predicting Stable Ratios of Stock Prices. Econometrics 2016, 4, 14
Authors: Ardia David , Gatarek Lukasz T. , Hoogerheide Lennart , Van Dijk Herman K.
Published: February 2020

The authors wish to make the following corrections to this paper (Ardia et al [...]
Mathematical Performance of American Youth: Diminished Returns of Educational Attainment of Asian-American Parents
Authors: Assari Shervin , Boyce Shanika , Bazargan Mohsen , Caldwell Cleopatra H.
Published: February 2020

The Marginalization-related Diminished Returns (MDR) phenomenon refers to the weaker effects of parental educational attainment for marginalized groups, particularly ethnic minorities. This literature, however, is limited to Blacks and Hispanics; thus, it is not clear if the MDR phenomenon also applies to the educational performance of Asian Americans or not. To explore ethnic differences in the association between parental educational attainment and youth mathematical performance among 10th-grade American high schoolers, this cross-sectional study used baseline data from the Education Longitudinal Study, a national survey of 10th-grade American youth. The analytical sample included a total number of 10,142 youth composed of 1460 (14.4%) Asian-American and 8682 (85.6%) non-Hispanic youth. The dependent variable was youth math performance (standard test score). The independent variable was parental education. Gender, both parents living in the same household, and school characteristics (% students receiving free lunch, urban school, and public school) were the covariates. Ethnicity was the moderating variable. Linear regression was used for data analysis. Overall, parental educational attainment was positively associated with math ability (test score). We observed a statistically significant interaction between ethnicity (Asian American) and parental education attainment on the results of math test scores, indicating that the boosting effect of high parental educational attainment on youth math function is smaller for Asian-American youth than for Non-Hispanic White youth. While high parental educational attainment contributes to youth educational outcomes, this association is weaker for Asian-American youth than non-Hispanic White youth. Diminished returns (weaker effects of parental education in generating outcomes for ethnic minorities) that are previously shown for Hispanics and Blacks also apply to Asian Americans.
The Organization of Self-Knowledge in Adolescence: Some Contributions Using the Repertory Grid Technique
Authors: Carapeto Maria João , Feixas Guillem
Published: February 2020

(1) Background: This study aims to explore the usefulness of personal construct psychology as a comprehensive framework and assessment tool to embrace a diversity of self-knowledge organization constructs, and to account for developmental differences across adolescence. (2) Methods: The repertory grid technique was used to measure self-knowledge differentiation, polarization, discrepancies between Actual Self, Ideal Self, and Others, and implicative dilemmas, a particular kind of intrapersonal conflict. Data were collected from two samples of early and late adolescents, respectively. (3) Results: Globally, they showed that the organization of self-knowledge was different in both samples. In particular, older adolescents revealed a less polarized self-knowledge. In addition, they tended to construe higher Actual–Ideal self-discrepancies and to present more internal conflicts. No differences were found between early and late adolescents concerning global differentiation and the discrepancies between the self (Actual and Ideal) and the Others. (4) Conclusions: Despite the limitations of the study (e.g., small sample size, cross-sectional design), these novel results support the suitability of the repertory grid technique to capture developmental changes in self-knowledge organization during adolescence, as well as the explanatory potential of personal construct psychology to advance their understanding.
A Comprehensive Assessment of Food Parenting Practices: Psychometric Properties of the Portuguese Version of the HomeSTEAD Family Food Practices Survey and Associations with Children’s Weight and Food Intake
Authors: Afonso Lisa , Castro Joana , Parente Nuno , Torres Sandra
Published: February 2020

The Home Self-Administered Tool for Environmental Assessment of Activity and Diet (HomeSTEAD) survey evaluates a broad spectrum of food parenting practices related to parental use of control, autonomy support, and structure. This study aims to test the psychometric properties of the Portuguese version of the HomeSTEAD family food practices survey in parents of 3–12 year old children. Data were collected from 184 parents/caregivers. We performed an exploratory factor analysis (EFA), calculated the internal consistency coefficients of each subscale, and tested for associations with children’s food intake and weight. Based on the EFA, 61 items were included in the Portuguese version of the HomeSTEAD family food practices survey, and were distributed among four Coercive Control Practices (16 items); five Autonomy Support Practices (17 items); and nine Structure Practices (28 items). All scales demonstrated an acceptable level of internal consistency. A higher body mass index (BMI) SD score in children was associated with higher levels of restriction and weight talk by parents and distractions during meals. Higher levels of distractions during meals were also associated with higher sweets intake in children. Additionally, higher levels of parental modeling and the establishment of rules and limits were associated with lower intake of sugar-sweetened beverages. These associations provide preliminary evidence of the HomeSTEAD family food practices survey’s construct validity and attest to its potential to assess parental strategies and provide useful information to improve children’s eating.
Interference Analysis for Vehicle-to-Vehicle Communications at 28 GHz
Authors: Saraereh Omar A. , Ali Ashraf , Khan Imran , Rabie Khaled
Published: February 2020

High capacity and ultra-reliable vehicular communication are going to be important aspects of beyond 5G communication networks. However, the vehicular communication problem becomes complex at a large scale when vehicles are roaming on the road, while simultaneously communicating with each other. Moreover, at higher frequencies (like 28 GHz), the dynamics of vehicular communication completely shift towards unpredictability and low-reliability. These factors may result in high packet error and a large amount of interference, resulting in regular disruptions in communications. A thorough understanding of performance variations is the key to moving towards the next generation of vehicular networks. With this intent, this article aims to provide a comprehensive interference analysis, wherein the closed-form expressions of packet error probability (PEP) and ergodic capacity are derived. Using the expression of the PEP, diversity analysis is provided which unveils the impact of channel nonlinearities on the performance of interference-constrained vehicular networks. The insights provided here are expected to pave the way for reliable and high capacity vehicular communication networks.
Research on Modified Hybrid Frequency Modulation Technology of Type-III Asymmetric CHB Multilevel Inverters
Authors: Ye Manyuan , Ren Wei , Wei Qiwen , Song Guizhi , Miao Zhilin
Published: February 2020

Asymmetric Cascaded H-bridge (ACHB) level inverters can output more voltage waveforms with fewer cascaded units while ensuring the quality of output voltage waveforms, so they have attracted more and more attention. Taking the topology of Type-III asymmetric CHB multilevel inverters as the research object, a Modified Hybrid Frequency Pulse Width Modulation (MHF-PWM) strategy is proposed in this paper. This modulation strategy overcomes the local overshoot of low-voltage unit in the presence of traditional Hybrid Frequency Pulse Width Modulation (HF-PWM), thus completely eliminating the low frequency harmonics in the output voltage waveform of Type-III ACHB nine-level inverters, and the Total Harmonic Distortion (THD) of output line voltage of the modulation strategy is lower than that of PS-PWM strategy in the whole modulation degree, which effectively improves the quality waveform of the output line voltage. At the same time, the strategy can also improve the problems of current backflow and energy feedback caused by the high-voltage unit pouring current to the low-voltage unit, thereby reducing the imbalance of the output power of the high-voltage and low-voltage units. Finally, the Matlab/Simulink simulation model and experimental platform are established to verify the validity and practicality of the modulation strategy.