bluuebunny/crossref_metadata_2025 huggingface dataset:
Escherichia coli from urine samples of pregnant women as an indicator for antimicrobial resistance in the community: a field study from rural Burkina Faso
Authors: Post Annelies S. , Guiraud I. , Peeters M. , Lompo P. , Ombelet S. , Karama I. , Yougbaré S. , Garba Z. , Rouamba E. , Tinto H. , Jacobs Jan
Published: September 2022

Background

In low- and middle-income countries, surveillance of antimicrobial resistance (AMR) is mostly hospital-based and, in view of poor access to clinical microbiology, biased to more resistant pathogens. We aimed to assess AMR among Escherichia coli isolates obtained from urine cultures of pregnant women as an indicator for community AMR and compared the AMR results with those from E. coli isolates obtained from febrile patients in previously published clinical surveillance studies conducted within the same population in Nanoro, rural Burkina Faso. We furthermore explored feasibility of adding urine culture to standard antenatal care in a rural sub-Saharan African setting.

Methods

Between October 2016–September 2018, midstream urine samples collected as part of routine antenatal care in Nanoro district were cultured by a dipslide method and screened for antibiotic residues. Significant growth was defined as a pure culture of Enterobacterales at counts of ≥ 104 colony forming units/ml.

Results

Significant growth was observed in 202/5934 (3.4%) cultures; E. coli represented 155 (76.7%) of isolates. Among E. coli isolates, resistance rates to ampicillin, cotrimoxazole and ciprofloxacin were respectively 65.8%, 64.4% 16.2%, compared to 89.5%, 89.5% and 62.5% among E. coli from clinical isolates (n = 48 of which 45 from blood cultures). Proportions of extended spectrum beta-lactamase producers and multidrug resistance were 3.2% and 5.2% among E. coli isolates from urine in pregnant women versus 35.4%, and 60.4% respectively among clinical isolates.

Conclusions

The E. coli isolates obtained from healthy pregnant women had significantly lower AMR rates compared to clinical E. coli isolates, probably reflecting the lower antibiotic pressure in the pregnant women population. Adding urine culture to the routine urine analysis (dipstick) of antenatal care was feasible. The dipslide culture method was affordable and user-friendly and allowed on-site inoculation and easy transport; challenges were contamination (midstream urine sampling) and the semi-quantitative reading. Provided confirmation of the present findings in other settings, E. coli from urine samples in pregnant women may be a potential indicator for benchmarking, comparing, and monitoring community AMR rates across populations over different countries and regions.
CircRIC8B regulates the lipid metabolism of chronic lymphocytic leukemia through miR199b-5p/LPL axis
Authors: Wu Zijuan , Gu Danling , Wang Ruixin , Zuo Xiaoling , Zhu Huayuan , Wang Luqiao , Lu Xueying , Xia Yi , Qin Shuchao , Zhang Wei , Xu Wei , Fan Lei , Li Jianyong , Jin Hui
Published: September 2022

Objective

Circular RNAs (circRNAs) play a critical role in the modulation of tumor metabolism. However, the expression patterns and metabolic function of circRNAs in chronic lymphocytic leukemia (CLL) remain largely unknown. This study aimed to elucidate the role of circRNAs in the lipid metabolism of CLL.

Methods

The expression and metabolic patterns of circRNAs in a cohort of 53 patients with CLL were investigated using whole transcriptome sequencing. Cell viability, liquid chromatography with tandem mass spectrometry (LC–MS/MS) analysis, lipid analysis, Nile red staining as well as triglyceride (TG) assay were used to evaluate the biological function of circRIC8B in CLL. The regulatory mechanisms of circRIC8B/miR-199b-5p/lipoprotein lipase (LPL) axis were explored by luciferase assay, RNA immunoprecipitation (RIP), qRT-PCR, and fluorescence in situ hybridization (FISH). CCK-8 and flow cytometry were used to verify the inhibition role of cholesterol absorption inhibitor, ezetimibe, in CLL cells.

Results

Increased circRIC8B expression was positively correlated with advanced progression and poor prognosis. Knockdown of circRIC8B significantly suppressed the proliferation and lipid accumulation of CLL cells. In contrast, the upregulation of circRIC8B exerted opposite effects. Mechanistically, circRIC8B acted as a sponge of miR-199b-5p and prevented it from decreasing the level of LPL mRNA, and this promotes lipid metabolism alteration and facilitates the progression of CLL. What’s more, ezetimibe suppressed the expression of LPL mRNA and inhibited the growth of CLL cells.

Conclusions

In this study, the expressional and metabolic patterns of circRNAs in CLL was illustrated for the 1st time. Our findings revealed that circRIC8B regulates the lipid metabolism abnormalities in and development of CLL through the miR-199b-5p/LPL axis. CircRIC8B may serve as a promising prognostic marker and therapeutic target, which enhances the sensitivity to ezetimibe in CLL.
Corncob structures in dental plaque reveal microhabitat taxon specificity
Authors: Morillo-Lopez Viviana , Sjaarda Alexandra , Islam Imon , Borisy Gary G. , Mark Welch Jessica L.
Published: September 2022

Background

The human mouth is a natural laboratory for studying how bacterial communities differ across habitats. Different bacteria colonize different surfaces in the mouth—teeth, tongue dorsum, and keratinized and non-keratinized epithelia—despite the short physical distance between these habitats and their connection through saliva. We sought to determine whether more tightly defined microhabitats might have more tightly defined sets of resident bacteria. A microhabitat may be characterized, for example, as the space adjacent to a particular species of bacterium. Corncob structures of dental plaque, consisting of coccoid bacteria bound to filaments of Corynebacterium cells, present an opportunity to analyze the community structure of one such well-defined microhabitat within a complex natural biofilm. Here, we investigate by fluorescence in situ hybridization and spectral imaging the composition of the cocci decorating the filaments.

Results

The range of taxa observed in corncobs was limited to a small subset of the taxa present in dental plaque. Among four major groups of dental plaque streptococci, two were the major constituents of corncobs, including one that was the most abundant Streptococcus species in corncobs despite being relatively rare in dental plaque overall. Images showed both Streptococcus types in corncobs in all individual donors, suggesting that the taxa have different ecological roles or that mechanisms exist for stabilizing the persistence of functionally redundant taxa in the population. Direct taxon-taxon interactions were observed not only between the Streptococcus cells and the central corncob filament but also between Streptococcus cells and the limited subset of other plaque bacteria detected in the corncobs, indicating species ensembles involving these taxa as well.

Conclusions

The spatial organization we observed in corncobs suggests that each of the microbial participants can interact with multiple, albeit limited, potential partners, a feature that may encourage the long-term stability of the community. Additionally, our results suggest the general principle that a precisely defined microhabitat will be inhabited by a small and well-defined set of microbial taxa. Thus, our results are important for understanding the structure and organizing principles of natural biofilms and lay the groundwork for future work to modulate and control biofilms for human health.
Investigating the effect of classroom-based feedback on speaking assessment: a multifaceted Rasch analysis
Authors: Bijani Houman , Hashempour Bahareh , Ibrahim Khaled Ahmed Abdel-Al , Orabah Salim Said Bani , Heydarnejad Tahereh
Published: September 2022

Due to subjectivity in oral assessment, much concentration has been put on obtaining a satisfactory measure of consistency among raters. However, the process for obtaining more consistency might not result in valid decisions. One matter that is at the core of both reliability and validity in oral assessment is rater training. Recently, multifaceted Rasch measurement (MFRM) has been adopted to address the problem of rater bias and inconsistency in scoring; however, no research has incorporated the facets of test takers’ ability, raters’ severity, task difficulty, group expertise, scale criterion category, and test version together in a piece of research along with their two-sided impacts. Moreover, little research has investigated how long rater training effects last. Consequently, this study explored the influence of the training program and feedback by having 20 raters score the oral production produced by 300 test-takers in three phases. The results indicated that training can lead to more degrees of interrater reliability and diminished measures of severity/leniency, and biasedness. However, it will not lead the raters into total unanimity, except for making them more self-consistent. Even though rater training might result in higher internal consistency among raters, it cannot simply eradicate individual differences related to their characteristics. That is, experienced raters, due to their idiosyncratic characteristics, did not benefit as much as inexperienced ones. This study also showed that the outcome of training might not endure in long term after training; thus, it requires ongoing training throughout the rating period letting raters regain consistency.
Portrait of an artist at work: exploring Max Ernst’s surrealist techniques
Authors: Zuena Martina , Pensabene Buemi Luciano , Nodari Luca , Subelytė Grazina , Stringari Lena , Campanella Beatrice , Lorenzetti Giulia , Palleschi Vincenzo , Tomasin Patrizia , Legnaioli Stefano
Published: September 2022

Max Ernst was one of the most influential artists associated with both the Dada and Surrealist movements. However, until now, only few scientific studies have been devoted to his works. This paper presents the results of a multi-analytical investigation on six oil paintings, made between 1927 and 1942, belonging to the Peggy Guggenheim Collection in Venice (Solomon R. Guggenheim Foundation, New York). Through a combined art historical and scientific approach, this study aims at understanding Ernst’s painting techniques, including frottage, grattage, dripping, and decalcomania, the used materials, and the state of conservation of the artworks. Non-invasive in situ investigations were performed by means of Vis–NIR multi-spectral imaging, X-ray fluorescence, external reflection FTIR and Raman spectroscopy. Imaging analysis revealed important information about Ernst’s painting methods while the other techniques provided useful information about the ground layer, the painting materials and the presence of alteration products. Ernst’s palette discloses great freedom in his use of materials and evolution during the time. This investigation demonstrates that an integrated, non-invasive, diagnostic approach provides a thorough analysis of materials and execution techniques of Ernst’ masterworks allowing an in-depth knowledge of his highly skilled work.
Exploration of facilitators and barriers to the regulatory frameworks of dietary and herbal supplements: a scoping review
Authors: Ng Jeremy Y. , Kim Minji , Suri Ayush
Published: September 2022

Background

Over the last decade, the use of dietary and herbal supplements (DHSs) has expanded greatly across national healthcare settings for the prevention, management and/or treatment of a variety of health issues. Although regulatory policies for DHSs across different countries have been aimed towards evaluating safety and efficacy, performing quality controls, monitoring the manufacturing practices, and encouraging further research, significant safety issues have emerged from inappropriate regulatory classification, lack of suitable quality controls, and inadequate testing and labeling of these products. Therefore, the purpose of this scoping review was to identify facilitators and barriers to DHS regulation across different countries.

Methods

A scoping review was conducted and informed by the five-stage methodological framework proposed by Arksey and O’Malley and further enhanced by Levac et al. MEDLINE, EMBASE, AMED, and PsycINFO databases were systematically searched for eligible articles from database inception to September 29, 2020. Studies analyzing DHS regulatory frameworks were eligible for review. Relevant data from eligible articles were extracted and categorized into themes to provide a descriptive overview of the literature.

Results

Searches generated 4314 results, 1800 of which were duplicates, followed by 2472 that were excluded after screening the titles and abstracts of these articles. Of the remaining 42 full-texts, 15 eligible articles were included in this review. Themes identified include: (1) increased financial and human resources encouraging knowledge expansion as a facilitator to DHS regulation; (2) variances in DHS classification and regulatory requirements across countries as a barrier to DHS regulation, and (3) collaboration between various stakeholders (experts, policymakers, representatives of regulatory bodies, product companies and research institutions) facilitating DHS regulation.

Conclusion

The present scoping review identified facilitators and barriers to DHS regulation across different countries. We highlight that safety assessments of DHSs continue to be inadequate, and emerging technologies could potentially play a significant role in establishing common reference standards of herbal materials and products between regulatory agencies. Regulatory harmonization, increased scientific research, and collaboration could improve regulations globally through appropriate categorization and safe application of DHSs.
Association between ICU admission during off-hours and in-hospital mortality: a multicenter registry in Japan
Authors: Namikata Yu , Matsuoka Yoshinori , Ito Jiro , Seo Ryutaro , Hijikata Yasukazu , Itaya Takahiro , Ouchi Kenjiro , Nishida Haruka , Yamamoto Yosuke , Ariyoshi Koichi
Published: September 2022

Background

The effect of ICU admission time on patient outcomes has been shown to be controversial in several studies from a number of countries. The imbalance between ICU staffing and medical resources during off-hours possibly influences the outcome for critically ill or injured patients. Here, we aimed to evaluate the association between ICU admission during off-hours and in-hospital mortality in Japan.

Methods

This study was an observational study using a multicenter registry (Japanese Intensive care PAtient Database). From the registry, we enrolled adult patients admitted to ICUs from April 2015 to March 2019. Patients with elective surgery, readmission to ICUs, or ICU admissions only for medical procedures were excluded. We compared in-hospital mortalities between ICU patients admitted during off-hours and office-hours, using a multilevel logistic regression model which allows for the random effect of each hospital.

Results

A total of 28,200 patients were enrolled with a median age of 71 years (interquartile range [IQR], 59 to 80). The median APACHE II score was 18 (IQR, 13 to 24) with no significant difference between patients admitted during off-hours and those admitted during office-hours. The in-hospital mortality was 3399/20,403 (16.7%) when admitted during off-hours and 1604/7797 (20.6%) when admitted during office-hours. Thus, off-hours ICU admission was associated with lower in-hospital mortality (adjusted odds ratio 0.91, [95% confidence interval, 0.84–0.99]).

Conclusions

ICU admissions during off-hours were associated with lower in-hospital mortality in Japan. These results were against our expectations and raised some concerns for a possible imbalance between ICU staffing and workload during office-hours. Further studies with a sufficient dataset required for comparing with other countries are warranted in the future.
Understanding how facilitators adapt to needs of STEM faculty in online learning communities: a case study
Authors: Martin Makenna M. , Goldberg Fred , McKean Michael , Price Edward , Turpen Chandra
Published: September 2022

Background

Faculty Learning Communities (FLCs) and Faculty Online Learning Communities (FOLCs) are ways to support STEM faculty implementing research-based curricula. In these communities, faculty facilitators take on the role of sharing expertise and promoting discussion. However, as members gain more experience, their needs change from addressing logistical to pedagogical issues. Hence, facilitators need to change their practices in response. However, there is little research on the mechanisms of faculty facilitator change. In this article, we provide a case study of a specific STEM FOLC facilitator and demonstrate the usefulness of a teacher change model to investigate facilitator change.

Results

Guided by our adaptation of the InterconnectedModelofProfessionalGrowth(IMPG), we conducted interviews with FOLC facilitators, and selected a case facilitator who reported changes in facilitation goals and strategies over time. The model helped us identify specific areas of change and potential mechanisms for these changes. Using themes of change identified in the case facilitator interview, we developed coding schemes to analyze his FOLC meetings over a 2-year period. We found empirical evidence from multiple data sources, including FOLC meetings and facilitator reflections, that supported the change themes, including: changing his role as an “expert” by sharing his own expertise less and drawing on others’ expertise more frequently, changing his response to members’ comments by jumping in to answer less frequently and withholding his own responses more often to encourage member sharing, and a change in group discussions towards less logistical and more pedagogical conversations.

Conclusions

Our findings suggest that the IMPG can be fruitfully adapted to study facilitator change. A diagrammatic representation of the IMPG provides a description the types of change the case facilitator experienced and the factors that supported those changes. We discuss how the methodology used to analyze facilitator actions in FOLC group meetings may be useful to study other types of professional growth. Finally, because our analytical model allowed us to identify mechanisms of facilitator change, we describe the implications and provide suggestions to support facilitators in other faculty community groups.
Investigating the impact of peer supplemental instruction on underprepared and historically underserved students in introductory STEM courses
Authors: Anfuso Chantelle , Awong-Taylor Judy , Curry Savage Jamye , Johnson Cynthia , Leader Tirza , Pinzon Katherine , Shepler Benjamin , Achat-Mendes Cindy
Published: September 2022

Background

Supplemental instruction (SI) is a well-established mode of direct academic support, used in a wide variety of courses. Some reports have indicated that SI and similar peer-led academic support models particularly benefit students identifying with historically underserved racial/ethnic groups in STEM. However, these studies have not explicitly examined the role of prior academic experiences, an important consideration in college success. We report on the impact of a modified SI model, Peer Supplemental Instruction (PSI), on student success in introductory STEM courses at a diverse access institution. This study focuses on PSI’s impact on the academic performance of students identifying with historically underserved racial/ethnic groups, while also considering the effects of prior academic experiences.

Results

Data were aggregated for nine courses over five semesters to produce a robust data set (n = 1789). PSI attendees were representative of the overall student population in terms of previous academic experiences/performance (as determined by high school GPA) and self-identified racial/ethnic demographics. Frequent PSI attendance was correlated with a significant increase in AB rates (average increase of 29.0 percentage points) and reduction in DFW rates (average decrease of 26.1 percentage points) when comparing students who attended 10 + vs. 1–2 PSI sessions. Overall, students identifying as Black/African American received the largest benefit from PSI. These students experienced a significant increase in their final course GPA when attending as few as 3–5 PSI sessions, and exhibited the largest increase in AB rates (from 28.7 to 60.5%) and decrease in DFW rates (from 47.1 to 14.8%) when comparing students who attended 10 + vs. 1–2 sessions. However, students with similar HS GPAs experienced similar benefits from PSI, regardless of self-identified race/ethnicity.

Conclusions

The data presented here suggest that PSI particularly benefitted underprepared students in their introductory STEM courses. Since students identifying with historically underserved racial/ethnic groups have traditionally had inequitable K–12 educational experiences, they enter college less prepared on average, and thus particularly benefit from PSI. PSI, in conjunction with additional strategies, may be a useful tool to help rectify the results of systemic educational inequities for students identifying with historically underserved racial/ethnic groups.
Effect of chitooligosaccharides with a specific degree of polymerization on multiple targets in T2DM mice
Authors: You Jiangshan , Zhao Mengyao , Chen Shumin , Jiang Lihua , Gao Shuhong , Yin Hao , Zhao Liming
Published: September 2022

Chitooligosaccharides (COS) are found naturally in the ocean and present a variety of physiological activities, of which hypoglycemic action has attracted considerable research attention. This study aimed to assess the therapeutic effect of COS on mice suffering from type 2 diabetes mellitus (T2DM). COS effectively reduced blood glucose and blood lipid levels and improved glucose tolerance. Furthermore, COS revealed strong inhibitory activity against α-glucosidase, reducing postprandial blood glucose levels. Molecular docking data showed that COS might interact with surrounding amino acids to form a complex and decrease α-glucosidase activity. Additionally, COS enhanced insulin signal transduction and glycogen synthesis while restricting gluconeogenesis in the liver and muscles, reducing insulin resistance (IR) as a result. Moreover, COS effectively protected and restored islet cell function to increase insulin secretion. These results indicated that COS exhibited a significant hypoglycemic effect with multi-target participation. Therefore, COS may serve as a new preventive or therapeutic drug for diabetes to alleviate metabolic syndrome.

Graphical Abstract
Comparison of image quality and spatial resolution between 18F, 68Ga, and 64Cu phantom measurements using a digital Biograph Vision PET/CT
Authors: Braune Anja , Oehme Liane , Freudenberg Robert , Hofheinz Frank , van den Hoff Jörg , Kotzerke Jörg , Hoberück Sebastian
Published: September 2022

Background

PET nuclides can have a considerable influence on the spatial resolution and image quality of PET/CT scans, which can influence diagnostics in oncology, for example. The individual impact of the positron energy of 18F, 68Ga, and 64Cu on spatial resolution and image quality was compared for PET/CT scans acquired using a clinical, digital scanner.

Methods

A Jaszczak phantom and a NEMA PET body phantom were filled with 18F-FDG, 68Ga-HCl, or 64Cu-HCl, and PET/CT scans were performed on a Siemens Biograph Vision. Acquired images were analyzed regarding spatial resolution and image quality (recovery coefficients (RC), coefficient of variation within the background, contrast recovery coefficient (CRC), contrast–noise ratio (CNR), and relative count error in the lung insert). Data were compared between scans with different nuclides.

Results

We found that image quality was comparable between 18F-FDG and 64Cu-HCl PET/CT measurements featuring similar maximal endpoint energies of the positrons. In comparison, RC, CRC, and CNR were degraded in 68Ga-HCl data despite similar count rates. In particular, the two smallest spheres of 10 mm and 13 mm diameter revealed lower RC, CRC, and CNR values. The spatial resolution was similar between 18F-FDG and 64Cu-HCl but up to 18% and 23% worse compared with PET/CT images of the NEMA PET body phantom filled with 68Ga-HCl.

Conclusions

The positron energy of the PET nuclide influences the spatial resolution and image quality of a digital PET/CT scan. The image quality and spatial resolution of 68Ga-HCl PET/CT images were worse than those of 18F-FDG or 64Cu-HCl despite similar count rates.
Towards accurate partial volume correction in 99mTc oncology SPECT: perturbation for case-specific resolution estimation
Authors: Gillen Rebecca , Erlandsson Kjell , Denis-Bacelar Ana M. , Thielemans Kris , Hutton Brian F. , McQuaid Sarah J.
Published: September 2022

Background

Currently, there is no consensus on the optimal partial volume correction (PVC) algorithm for oncology imaging. Several existing PVC methods require knowledge of the reconstructed resolution, usually as the point spread function (PSF)—often assumed to be spatially invariant. However, this is not the case for SPECT imaging. This work aimed to assess the accuracy of SPECT quantification when PVC is applied using a case-specific PSF.

Methods

Simulations of SPECT$$^{99{\mathrm{m}}}$$99mTc imaging were performed for a range of activity distributions, including those replicating typical clinical oncology studies. Gaussian PSFs in reconstructed images were estimated using perturbation with a small point source. Estimates of the PSF were made in situations which could be encountered in a patient study, including; different positions in the field of view, different lesion shapes, sizes and contrasts, noise-free and noisy data. Ground truth images were convolved with the perturbation-estimated PSF, and with a PSF reflecting the resolution at the centre of the field of view. Both were compared with reconstructed images and the root-mean-square error calculated to assess the accuracy of the estimated PSF. PVC was applied using Single Target Correction, incorporating the perturbation-estimated PSF. Corrected regional mean values were assessed for quantitative accuracy.

Results

Perturbation-estimated PSF values demonstrated dependence on the position in the Field of View and the number of OSEM iterations. A lower root mean squared error was observed when convolution of the ground truth image was performed with the perturbation-estimated PSF, compared with convolution using a different PSF. Regional mean values following PVC using the perturbation-estimated PSF were more accurate than uncorrected data, or data corrected with PVC using an unsuitable PSF. This was the case for both simple and anthropomorphic phantoms. For the simple phantom, regional mean values were within 0.7% of the ground truth values. Accuracy improved after 5 or more OSEM iterations (10 subsets). For the anthropomorphic phantoms, post-correction regional mean values were within 1.6% of the ground truth values for noise-free uniform lesions.

Conclusion

Perturbation using a simulated point source could potentially improve quantitative SPECT accuracy via the application of PVC, provided that sufficient reconstruction iterations are used.
TRIM46 aggravated high glucose-induced hyper permeability and inflammatory response in human retinal capillary endothelial cells by promoting IκBα ubiquitination
Authors: Shen Hangqi , Gong Qiaoyun , Zhang Jingting , Wang Haiyan , Qiu Qinghua , Zhang Jingfa , Luo Dawei
Published: September 2022

Background

Diabetic retinopathy (DR) as a severe diabetic complication contributes to blindness. The increased permeability of retinal capillary endothelial cells (RCECs) as well as the production of inflammatory markers are closely related to DR occurrence. We recently revealed that TRIM46 promotes high glucose (HG)-caused ferroptosis in human RCECs (HRCECs). The current study aims to explore the molecular mechanism of how TRIM46 plays its role in DR progression.

Methods

Western blot was utilized to determine protein expression. The cell counting kit-8 assay was used to observe cell viability. The permeability of the cell layer was determined by measuring the transepithelial electrical resistance and fluorescein isothiocyanate (FITC)-dextran leak. Enzyme-linked immunosorbent assay was used to quantify the protein level of pro-inflammatory cytokines and co-immunoprecipitation was employed to verify the relationship between TRIM46 and IκBα.

Results

HG dramatically upregulated TRIM46 protein expression in a dose-dependent way. Silencing TRIM46 effectively reversed HG-induced cell growth inhibition, cell cycle arrest, hyper permeability and pro-inflammatory cytokines secretion in HRCECs, while overexpression of TRIM46 exhibited an opposite effect. Furthermore, TRIM46 was able to interact with IκBα and promote the ubiquitination and degradation of IκBα. IκBα overexpression recovered the effects of TRIM46 overexpression in HRCECs. Furthermore, inhibiting the activation of NF-κB partially recovered HG-induced HRCEC injury, whereas TRIM46 overexpression reversed these effects.

Conclusion

This study demonstrates that TRIM46 interacts with IκBα to activate the NF-κB signaling pathway, thereby enhancing cell proliferation inhibition, hyper permeability and the inflammatory response of HRCECs in a HG state.
Application of a Reactive Agility Training Program Using Light-Based Stimuli to Enhance the Physical and Cognitive Performance of Car Racing Drivers: A Randomized Controlled Trial
Authors: Horváth Dávid , Négyesi János , Győri Tamás , Szűcs Botond , Tóth Péter János , Matics Zsolt , Ökrös Csaba , Sáfár Sándor , Szabó Nikolett , Takács Beáta , Kathy Róbert , Tóth Klára , Ferguson David P. , Nagatomi Ryoichi , Rácz Levente
Published: September 2022

Background

There is a need to develop strategies that could contribute to the physical and mental preparation of motorsport athletes. A common method used by experienced motorsport athlete physical trainers is flashing light devices to train or assess reactive agility, despite limited evidence. Therefore, in the present study, we determined the effects of a 6-week reactive agility training program using light-based stimuli on the physiological and cognitive abilities of car racing drivers.

Materials and Methods

The CONSORT guidelines for randomized controlled trial were used. In a single-blinded randomized controlled trial, 24 car racing drivers (EXP, n = 12; CON, n = 12) performed a comprehensive battery of cognitive tests marketed specifically at motorsport athletes from Vienna test system (VTS) at rest or during moderate intensity exercise on a bicycle. Physiological abilities were determined via a maximal incremental cardio-respiratory treadmill test. Baseline and post-intervention tests were performed on three consecutive days. Participants in EXP underwent a 6-week intervention consisting of 60-min training sessions twice a week using the Witty SEM light stimulus.

Results

Participants in EXP but not in CON performed some of the VTS cognitive tasks with higher accuracy and/or shorter reaction time after the intervention at rest and during exercise. Car racing drivers performed the STROOP word-reading condition more accurately when the task was performed during the exercise vs. rest, regardless of group. In addition, the intervention induced beneficial changes in peak heart rate (HR), HR at gas exchange threshold, ventilation, and relative maximal oxygen consumption (rVO2 max). In contrast, body mass and fat mass increased, while peak HR and rVO2 max decreased in CON. Finally, participants in EXP improved their reactive agility performance and reaction time throughout the training program.

Conclusion

Overall, the reactive agility training program using light-based stimuli appeared to be efficient to induce beneficial effects on some physiological and cognitive performance measures; therefore, it may have the potential to contribute to car racing drivers’ physical and mental performance.
Impact of COVID-19 on G20 countries: analysis of economic recession using data mining approaches
Authors: Taylan Osman , Alkabaa Abdulaziz S. , Yılmaz Mustafa Tahsin
Published: September 2022

The G20 countries are the locomotives of economic growth, representing 64% of the global population and including 4.7 billion inhabitants. As a monetary and market value index, real gross domestic product (GDP) is affected by several factors and reflects the economic development of countries. This study aimed to reveal the hidden economic patterns of G20 countries, study the complexity of related economic factors, and analyze the economic reactions taken by policymakers during the coronavirus disease of 2019 (COVID-19) pandemic recession (2019–2020). In this respect, this study employed data-mining techniques of nonparametric classification tree and hierarchical clustering approaches to consider factors such as GDP/capita, industrial production, government spending, COVID-19 cases/population, patient recovery, COVID-19 death cases, number of hospital beds/1000 people, and percentage of the vaccinated population to identify clusters for G20 countries. The clustering approach can help policymakers measure economic indices in terms of the factors considered to identify the specific focus of influences on economic development. The results exhibited significant findings for the economic effects of the COVID-19 pandemic on G20 countries, splitting them into three clusters by sharing different measurements and patterns (harmonies and variances across G20 countries). A comprehensive statistical analysis was performed to analyze endogenous and exogenous factors. Similarly, the classification and regression tree method was applied to predict the associations between the response and independent factors to split the G-20 countries into different groups and analyze the economic recession. Variables such as GDP per capita and patient recovery of COVID-19 cases with values of $12,012 and 82.8%, respectively, were the most significant factors for clustering the G20 countries, with a correlation coefficient (R2) of 91.8%. The results and findings offer some crucial recommendations to handle pandemics in terms of the suggested economic systems by identifying the challenges that the G20 countries have experienced.
Vitrectomy for macular retinoschisis associated with peripapillary intrachoroidal cavitations in a moderately myopic eye
Authors: Aoki Shuichiro , Imaizumi Hiroko
Published: September 2022

Background

Peripapillary intrachoroidal cavitation (PICC), a cavernous change in the peripapillary choroid, may present with macular lesions. Here, we present a rare case of PICC with outer lamellar macular hole and macular retinoschisis.

Case presentation

A 69-year-old man presented with metamorphopsia in the left eye. Fundus examination revealed macular retinoschisis and PICCs. Pars plana vitrectomy with fovea sparing internal limiting membrane peeling (FSIP) was performed. Three weeks postoperatively, the patient developed rhegmatogenous retinal detachment and underwent a second vitrectomy. The macular retinoschisis resolved without macular hole formation and the patient’s visual acuity improved. There were no recurrent macular lesions over the three years of postoperative follow-up. Postoperative spectral-domain optical coherence tomography scan revealed a communication between the PICC and the vitreous cavity.

Conclusions

This case shed light on understanding development of PICC in non-highly myopic eyes and a rare complication of macular retinoschisis. Vitrectomy with FSIP may effectively resolve macular retinoschisis.
Particulates formed in dialysis fluid: effect of silica in tap water
Authors: Imai Motoyuki , Yamamoto Masahito , Togo Konomi , Yamashita Akihiro C.
Published: September 2022

Background

A fluid commonly used for dialysis in Japan is prepared from two dialysate concentrates and dialysis water. Silicon (Si) from tap water is reported in dialysis water as suspended insoluble particulate matters. We aimed to examine the source of insoluble particulate matters in the online prepared substitution fluid and to examine effects of the silica (SiO2) of tap water on the SiO2 of dialysis water.

Method

Based on the number of particles and particle size distribution in online prepared substitution fluid, we evaluated particle diameters and constituent elements of particles in online prepared substitution fluid. To identify sources of insoluble particulate matters in the online prepared substitution fluid, we measured particle diameters and constituent elements of particles adhering to the surface of hollow fiber of the final endotoxin-retentive filter (ETRF) and to the inner surface of the housing of the final ETRF and measured SiO2 in tap water, dialysis water, and commercial sterile substitution fluid.

Results

Si-containing particles accounted for 83% of 30 particles detected in the final ETRF (the hollow fibers and the housing) and the online prepared substitution fluid sampled from the final ETRF. The large variability in the SiO2 in tap water depending on the geographic regions. The SiO2 in dialysis water was 0.4–0.7 mg/L. Si was not detected in particles in the commercial sterilized substitution fluid, and the commercial sterile substitution fluids contained SiO2 at 0.1 mg/L.

Conclusion

We presume that insoluble particulates in online prepared substitution fluid were calcium and magnesium precipitated from dialysate and Si precipitated from dialysis water. Moreover, variation in the SiO2 in tap water is a factor affecting the SiO2 level in dialysis water. If the formation of silicates in the online prepared substitution fluid is considered and the target SiO2 in dialysis water is to be set ≤ 0.1 mg/L, SiO2 in tap water available in hospitals and dialysis centers should be known before specifications of instruments for dialysis fluid preparation are determined.
Efficacy and safety of endovascular therapy for delayed hepatic artery post-pancreatectomy hemorrhage: development of extrahepatic collateral circulation and complications of post endovascular therapy
Authors: Nozawa Yosuke , Ymazoe Shinji , Masuda Koichi , Takigawa Yutaka , Kobashi Yuko , Ikeda Koshi , Fukuda Takeshi , Michimoto Kenkichi
Published: September 2022

Background

Endovascular therapy (ET) for delayed hepatic artery post-pancreatectomy hemorrhage (HA-PPH) may require complete hepatic artery occlusion (HAO). Nonetheless, the development of extrahepatic collateral circulation (EHC) and the relationship between radiological factors (EHC, portal vein stenosis, and HAO) and adverse hepatic events after ET remain unclear. This study aimed to evaluate the efficacy and safety of ET for delayed PPH and examine the development of EHC.

Methods

A total of 19 ET cases for delayed HA-PPH were reviewed. Hepatic adverse events, portal vein stenosis, HAO, and mortality rate after ET were evaluated. Moreover, EHC from the left gastric artery (LGA), right inferior phrenic artery (RIPA), left inferior phrenic artery (LIPA), right internal thoracic artery (RITA), left internal thoracic artery (LITA), renal artery (RA), omental artery (OA), intercostal artery (IA), and branch of superior mesenteric artery (BSMA) was assessed using angiogram and computed tomography angiography (CTA).

Results

All cases were successfully treated using transcatheter arterial embolization (n = 17) and stent-graft placement (n = 2) without mortality. EHC from the LGA (8/19), RIPA (10/19), LIPA (4/19), and RITA (3/19) was observed on post-ET CTA. The incidence of hepatic adverse events was significant in the group with both HAO and portal vein stenosis (p < 0.001) and absence of EHC from LIPA and RITA (p < 0.05).

Conclusion

ET for delayed HA-PPH might be effective and safe. While avoiding both HAO and portal vein stenosis is important, the development of EHCs from LIPA and RITA may prevent hepatic adverse events after ET.
The value of data privacy during the COVID-19 pandemic: a new set of survey questions
Authors: Helmschrott Susanne , Anand Kartik , Zhou Sophie , Schmidt Tobias
Published: September 2022

Contact-tracing smartphone apps that rely on users’ private data have been proposed as important tools in fighting the COVID-19 pandemic. The use of these apps, however, has sparked new debates on the value of data privacy. Several earlier studies have investigated citizens’ willingness to use such apps. We propose a set of questions as a new measurement instrument that goes beyond eliciting acceptance and aims at quantifying users’ willingness to pay (WTP) for data privacy. We assess some aspects of the measurement instrument pertaining to its validity. We find central assumptions of our theoretical model met, suggesting that the instrument serves as a good starting point for measuring WTP. For example, we found a rather low WTP for data privacy in times of a pandemic, with high consent rates to data sharing and a majority of people who would pay amounts of up to 10€ only to not have to share data. Nevertheless, there are several improvements to the instrument possible that should be addressed by future research. We also encourage researchers to field the refined version in larger samples including the offline population.
Essen transition model for neuromuscular diseases
Authors: Fleischer Michael , Coskun Bayram , Stolte Benjamin , Della-Marina Adela , Kölbel Heike , Lax Hildegard , Nonnemacher Michael , Kleinschnitz Christoph , Schara-Schmidt Ulrike , Hagenacker Tim
Published: September 2022

Background

With the optimization of medical care structures and the rapid progress in the development of new therapeutic methods, an increase in life expectancy is observed in patients with neuromuscular diseases. This leads to an expansion of the phenotypic spectrum, whereby new or previously less relevant disease manifestations in different organ systems gain more importance. The care of adolescents and young adults with neuromuscular diseases, therefore, requires increasingly close interdisciplinary collaboration within neuromuscular centers.

Research question

How can the transition process from pediatric to adult care be structured so that the individual disciplines are efficiently integrated into the complex treatment and care process, and the patients' quality of life is improved?

Material and methods

A structured transition process was established at the University Hospital in Essen, Germany. Exemplarily, a comparable care concept was developed based on Pompe disease, Duchenne muscular dystrophy, and juvenile myasthenia gravis comprising four elements: (1) With the introduction of cross-department standard operating procedures, the logistical processes, as well as the diagnostic and therapeutic measures, are uniformly coordinated, and the transition process is bindingly defined. (2) To ensure a seamless transition, young patients are seen with their parents during joint consultations before they reach their 17th birthday. This creates an opportunity for patients to get to know the subsequent department structure and build a lasting relationship of trust. (3) A quarterly “transition board” regularly brings together the participating disciplines from pediatric and adult care systems for a case-related interdisciplinary exchange and continuous optimization of the transition process. (4) A cross-department “Transition Database”, in which medical findings and parameters are recorded, was implemented as a common information platform and database.

Conclusion

The Essen Transition Model aims to close the gap in care for young patients with neuromuscular diseases during the critical transition from pediatric to adult medicine and to create a successful continuation of treatment in adulthood.
The use of coconut in rituals and food preparations in India: a review
Authors: Devi Monika , Ghatani Kriti
Published: September 2022

India is highly diverse ranging from snow-capped mountains to deserts, plateaus, and plain regions. It has a tropical climate with diverse climatic condition that favors the cultivation of wide range of fruit, vegetables, cereals, pulses, cotton, and various crops of medicinal importance making it one of the top producers in the world. India ranks second in the production of coconut grown in coastal regions playing a significant role in the agrarian economy and is known as a Nature’s gift to mankind; it is a source of food, beverage, oil seed, fibers, timbers, and health products. Further, it is a highly appreciated fruit with antibacterial, antifungal, antioxidant, antiparasitic, antiviral, and many other qualities. Considering the growing increase in consumption of coconut and its products and the large number of studies that address the theme, it is felt necessary to carry out a bibliographic survey based on the most current and relevant works in the area using books, journal articles, and other available print and electronic resources. This study attempts to gather information on a detailed assessment of the coconut; its uses in rituals, its health benefits, and culinary preparations across India as the delicacies of immemorial ethnic fruit are of rising significance. The importance and significance of documenting this coconut ethnicity in Indian cuisine are an attempt to keep it relevant and take it to broader viewers who admire such unique delicacies. The study reveals coconut has a high value in Indian ritual, and the survey of pharmacological studies on its medicinal uses supports its beneficial properties to human health.
Integration into regional or global value chains and economic upgrading prospects: an analysis of the East African Community (EAC) bloc
Authors: Lwesya Francis
Published: September 2022

The aim of the study was to assess the integration into regional or global value chains and economic upgrading prospects in the East African Community (EAC) bloc. We conducted a comparative analysis of participation in global value chain (GVC) among EAC member states and assessed the determinants of economic upgrading in the region using UNCTAD-Eora GVC Panel data from 2005 to 2018. The results show that Kenya, Tanzania and Uganda are relatively better integrated through two channels of GVC participation, namely foreign value added (FVA) and domestic value added. However, for indirect-value added channel, Kenya had the highest score, the rest member states had relatively lower mean scores. The overall results show that EAC’s participation in GVC still resides in upstream low- and middle-value-added production activities, which limits its competitiveness compared to other regions. The empirical results show the positive and significant effect of domestic credit, foreign direct investment, quality of institutions and FVA on economic upgrading. However, a positive but insignificant association with economic upgrading was observed for human capital and GDP per capita. Infrastructure quality was negatively and significantly associated with economic upgrading. These results suggest that improving infrastructure connectivity and further strengthening institutional governance would reduce trade costs and promote greater investments, product and service diversification, leading to deeper economic upgrading in the region. At the policy level, the adoption of effective national and regional industrial policies would promote innovation and human capital development, attract foreign direct investment and help address market and coordination deficiencies in the region.