bluuebunny/crossref_metadata_2025 huggingface dataset:
The Profile of Belgian Osteopaths: A Cross-Sectional Survey
Authors: van Dun Patrick L.S. , Verbeeck Johan , Arcuri Lorenzo , Esteves Jorge E. , Cerritelli Francesco
Published: October 2022

Background: This study gives an update on the characteristics of Belgian osteopaths five years after the Benelux Osteosurvey. Additional new data were collected on their professional identity and views on the profession. Methods: All Belgian osteopaths who could be contacted (n = 1473) were invited to complete a voluntary, online-based, closed-ended survey distributed between May and September 2018. The survey, composed of 52 questions and seven sections, was formally translated from English to Dutch and French and adapted from the original version. Adult, self-defined osteopaths working in Belgium were eligible. Recruitment of participants was performed through all professional associations and the InterMutualistic Agency. Descriptive statistics were used to analyse the data. Results: The survey was completed by 332 osteopaths. Thirty-one per cent of the respondents were female. Almost all the respondents were self-employed (99.4%); half of them worked as part of a team (47.6%). Most respondents had a 5-year part-time training, and the majority had a previous academic degree, mainly in physical therapy (65.8%). According to respondents, most patients seek care for lumbar non-specific low back, pelvis and neck pain. Most respondents strongly define themselves as osteopaths and advertise themselves exclusively as osteopaths. Conclusions: This survey provided an update of the current characteristics of Belgian osteopathic practitioners and added new information on their professional identity and views on the profession. The information provided could contribute to the body of evidence used by stakeholders and policymakers in the future regulation of the profession in Belgium.
Green Hydrogen Blends with Natural Gas and Its Impact on the Gas Network
Authors: Ekhtiari Ali , Flynn Damian , Syron Eoin
Published: October 2022

With increasing shares of variable and uncertain renewable generation in many power systems, there is an associated increase in the importance of energy storage to help balance supply and demand. Gas networks currently store and transport energy, and they have the potential to play a vital role in longer-term renewable energy storage. Gas and electricity networks are becoming more integrated with quick-responding gas-fired power plants, providing a significant backup source for renewable electricity in many systems. This study investigates Ireland’s gas network and operation when a variable green hydrogen input from excess wind power is blended with natural gas. How blended hydrogen impacts a gas network’s operational variables is also assessed by modelling a quasi-transient gas flow. The modelling approach incorporates gas density and a compressibility factor, in addition to the gas network’s main pressure and flow rate characteristics. With an increasing concentration of green hydrogen, up to 20%, in the gas network, the pipeline flow rate must be increased to compensate for reduced energy quality due to the lower energy density of the blended gas. Pressure drops across the gas pipeline have been investigated using different capacities of P2H from 18 MW to 124 MW. The results show significant potential for the gas network to store and transport renewable energy as hydrogen and improve renewable energy utilisation without upgrading the gas network infrastructure.
Clinical and Epidemiological Features of Hospitalized and Ambulatory Patients with Human Monkeypox Infection: A Retrospective Observational Study in Portugal
Authors: Caria João , Pinto Raquel , Leal Ema , Almeida Vasco , Cristóvão Gonçalo , Gonçalves Ana Catarina , Torres Margarida , Santos Maria Beatriz , Pinheiro Hélder , Póvoas Diana , Seixas Diana , Lino Sara , Cardoso Orlando , Manata Maria José , Virgolino Ana , Maltez Fernando
Published: October 2022

Monkeypox, a neglected and re-emergent zoonotic disease caused by monkeypox virus (MPXV) infection, has been endemic in Central and Western Africa for decades. More recently, an outbreak has spread to a global level, occurring in sites with no previous reported cases and being clustered among men who have sex with men, suggesting new modes of transmission. There is an urgent need for research for a better understanding of the genomic evolution and changing epidemiology of the Orthopoxvirus group. Our work aimed to characterize the clinical and epidemiological features of a cohort of patients with MPXV infection in a Portuguese hospital, admitted between 5 May and 26 July 2022. In this retrospective observational study, aggregate data of a case series on the presentation, clinical course, and outcomes of confirmed MPXV infections are reported. The study included 40 men and 1 woman, with a mean age of 37.2 years old; 92.7% identified as men who have sex with men, 90.2% had unprotected sex or sex with multiple or anonymous partners in the previous month, and 39.0% reported to have had sex with an MPXV-confirmed case; 59.5% had previously known human immunodeficiency virus (HIV) infection, all of whom were under antiretroviral therapy, and no patients had acquired immunodeficiency syndrome (AIDS) criteria. About a quarter of patients were observed only a week after symptom onset. All patients had skin or mucosal lesions and the anogenital region was the most frequent lesion site. There were no statistically significant clinical differences between HIV-positive and negative individuals. Four patients were admitted to the inpatient clinic, two of whom had proctitis with difficult-to-manage anal pain. There were no reported deaths. Our findings suggest the sexual route as a relevant mode of transmission of MPXV and confirm the mostly benign presentation of this disease.
Trade-Off between COVID-19 Pandemic Prevention and Control and Economic Stimulus
Authors: Liu Fangfang , Ma Zheng , Wang Ziqing , Xie Shaobo
Published: October 2022

The coronavirus disease 2019 (COVID-19) pandemic has posed a severe threat to public health and economic activity. Governments all around the world have taken positive measures to, on the one hand, contain the epidemic spread and, on the other hand, stimulate the economy. Without question, tightened anti-epidemic policy measures restrain people’s mobility and deteriorate the levels of social and economic activity. Meanwhile, loose policy measures bring little harm to the economy temporarily but could accelerate the transmission of the virus and ultimately wreck social and economic development. Therefore, these two kinds of governmental decision-making behaviors usually conflict with each other. With the purpose of realizing optimal socio-economic benefit over the full duration of the epidemic and to provide a helpful suggestion for the government, a trade-off is explored in this paper between the prevention and control of the epidemic, and economic stimulus. First, the susceptible–infectious–recovered (SIR) model is introduced to simulate the epidemic dynamics. Second, a state equation is constructed to describe the system state variable—the level of socio-economic activity dominated by two control variables. Specifically, these two variables are the strengths of the measures taken for pandemic prevention and control, and economic stimulus. Then, the objective function used to maximize the total socio-economic benefit over the epidemic’s duration is defined, and an optimal control problem is developed. The statistical data of the COVID-19 epidemic in Wuhan are used to validate the SIR model, and a COVID-19 epidemic scenario is used to evaluate the proposed method. The solution is discussed in both static and dynamic strategies, according to the knowledge of the epidemic’s duration. In the static strategy, two scenarios with different strengths (in terms of anti-epidemic and economic stimulus measures) are analyzed and compared. In the dynamic strategy, two global optimization algorithms, including the dynamic programming (DP) and Pontryagin’s minimum principle (PMP), respectively, are used to acquire the solutions. Moreover, a sensitivity analysis of model parameters is conducted. The results demonstrate that the static strategy, which is independent of the epidemic’s duration and can be easily solved, is capable of finding the optimal strengths of both policy measures. Meanwhile, the dynamic strategy, which generates global optimal trajectories of the control variables, can provide the path that leads to attaining the optimal total socio-economic benefit. The results reveal that the optimal total socio-economic benefit of the dynamic strategy is slightly higher than that of the static strategy.
Are Children Harmed by Being Locked up at Home? The Impact of Isolation during the COVID-19 Pandemic on the Phenomenon of Domestic Violence
Authors: Grzejszczak Jagoda , Gabryelska Agata , Gmitrowicz Agnieszka , Kotlicka-Antczak Magdalena , Strzelecki Dominik
Published: October 2022

The phenomenon of violence against children is a very complex one. There are many types of child abuse, and they are culturally dependent to a significant degree. Although studies show that children generally only suffer from mild COVID-19 infection, some social restrictions introduced during the pandemic, such as home isolation, may have many severe consequences on the population’s mental health. Studies on this topic suggest that violence against children increased during lockdown due to the COVID-10 pandemic. This narrative review summarizes this available literature on the subject and discusses the different forms of violence against children, their cultural aspects, the impact of the COVID-19 pandemic on the phenomenon of violence, the long-term consequences of the above, and forms of assistance for abused minors.
Moderating Effects of Self-Esteem on the Relationship between Communication Anxiety and Academic Performance among Female Health College Students during the COVID-19 Pandemic
Authors: Al Awaji Nisreen , Zaidi Uzma , Awad Salwa S. , Alroqaiba Nouf , Aldhahi Monira I. , Alsaleh Hadel , Akil Shahnaz , Mortada Eman M.
Published: October 2022

Unprecedented quarantine due to COVID-19 exposes individuals to withdraw from face-to-face interactions, which may influence communication and self-esteem (SE). Therefore, the overarching aims of this study are to examine the communication apprehension levels among female college students, and thus to investigate the moderating role of self-esteem on the relationship between communication apprehension and academic achievement. In this cross-sectional study, 287 female college students completed the survey, which was circulated through email. The survey included the following questionnaires: General Health Characteristics, Rosenberg Self-esteem Scale, and Personal Report of Communication Apprehension Scale. The results showed that 28.2% of participants were categorized as having a high level of communication apprehension, and only 9.8% had a low level of communication apprehension. The SE reported an overall score of 24.3 ± 2.14, indicating a high self-esteem level among students. The students’ grade point average (GPA) was positively correlated with SE. However, self-esteem as a moderator variable had no significant effect on the relationships between all predictors and GPA. The finding of the study highlights the need to implement different strategies to enhance students’ group discussions, meetings, and interpersonal communication to ensure the best learning outcomes. Future studies are required to investigate gender-based disparities in the relationship between communication apprehension and SE.
Municipal Ethnic Composition and Disparities in COVID-19 Infections in New Jersey: A Blinder–Oaxaca Decomposition Analysis
Authors: Wang Yuqi , Reyes Laurent , Greenfield Emily A. , Allred Sarah R.
Published: October 2022

COVID-19 has disproportionally impacted Latinx and Black communities in the US. Our study aimed to extend the understanding of ethnic disparities in COVID-19 case rates by using a unique dataset of municipal case rates across New Jersey (NJ) during the first 17 months of the pandemic. We examined the extent to which there were municipal-level ethnic disparities in COVID-19 infection rates during three distinct spikes in case rates over this period. Furthermore, we used the Blinder–Oaxaca decomposition analysis to identify municipal-level exposure and vulnerability factors that contributed to ethnic disparities and how the contributions of these factors changed across the three initial waves of infection. Two clear results emerged. First, in NJ, the COVID-19 infection risk disproportionally affected Latinx communities across all three waves during the first 17 months of the pandemic. Second, the exposure and vulnerability factors that most strongly contributed to higher rates of infection in Latinx and Black communities changed over time as the virus, alongside medical and societal responses to it, also changed. These findings suggest that understanding and addressing ethnicity-based COVID-19 disparities will require sustained attention to the systemic and structural factors that disproportionately place historically marginalized ethnic communities at greater risk of contracting COVID-19.
Development of the Web-Based Spanish Version of the Barthel Index in Patients with Multiple Sclerosis
Authors: Aguilar-Zafra Sandra , del Corral Tamara , Valera-Calero Juan Antonio , Martín-Casas Patricia , Plaza-Manzano Gustavo , López-de-Uralde-Villanueva Ibai
Published: October 2022

Background: The aims of this study were to develop a web-based Spanish form of the Barthel index (BI), to evaluate its psychometric properties and stability over time (test-retest), and to determine minimal detectable change (MDC) in patients with multiple sclerosis (MS). Methods: Participants answered the BI on two forms (web-based and face-to-face interview), 7–10 days apart. The internal consistency was evaluated using Cronbach’s alpha, and intraclass correlation (ICC) and kappa (κ) coefficients were used to investigate the agreement between both forms. Results: 143 participants were included. The Spanish web-based form of the BI showed excellent agreement between both forms for each item (κ = 0.86 (0.79 to 0.92), and for total score (κ = 0.87 (0.81 to 0.93); ICC = 0.99 (0.98 to 0.99). The internal consistency was good–excellent (Cronbach’s alpha = 0.89 (0.86–0.91)). The stability over time was adequate, the agreement of each item was κ = 0.63 (0.52–0.74)), and for total score (ICC = 0.97), determining a MDC95 of 12.09 points. Conclusions: The Spanish web-based form of the BI is a valid and reliable tool to assess functionality and can be applied in both formats in patients with MS. A total score difference of more than 12 points was found to indicate a deterioration or improvement in the patient’s functionality.
Age and Gender Perspectives on Social Media and Technology Practices during the COVID-19 Pandemic
Authors: Chidiac Mary , Ross Christopher , Marston Hannah R. , Freeman Shannon
Published: October 2022

Few studies have examined social media and technology use during the COVID-19 pandemic in Canada. Therefore, the main research question and objective of this study was to examine similarities and differences in the influences of mobile technology and social media use on Canadians among different age groups and across gender during the COVID-19 pandemic. From June through October 2021, 204 persons completed a 72-item online survey. Survey questions encompassed COVID-19 pandemic experiences and technology use. Standardized measures including the Psychological Wellbeing measure, eHeals, and the UCLA V3 Loneliness scale were collected to examine the psychological influences of the COVID-19 pandemic. Findings showed that males under 50 years were most likely to self-isolate compared to the other demographic results of the study. Males reported using technology less than females but were more likely to report using technology to share information regarding COVID-19. Respondents under 50 years were also more likely to use smartphones/mobile phones as their most used mobile technology device, whereas respondents over 50 were more split between smartphones/mobile phones and computers/tablets as their most used device. Males scored higher on the UCLA loneliness scale and lower on the Psychological Wellbeing sub-scores compared to females. Further research should explore additional demographics in relation to broader aspects of digital skills across different age groups.
Sexual Function in Women with Breast Cancer: An Evidence Map of Observational Studies
Authors: Rodrigues-Machado Nelson , Quintana M. , Gómez-Gómez Raquel , Bonfill-Cosp Xavier
Published: October 2022

Breast cancer (BC) is the leading cause of cancer in women, and has implications for sexual function (SF). In this study, we used an evidence map to identify, describe, and organise the current available evidence regarding SF in women with BC. We searched the MEDLINE, PsycINFO, and CINAHL databases for observational studies assessing SF in women with BC published in English, Spanish, Portuguese, and French between 2000 and 2021 (sample ≥ 50 women). Of the 64 included studies (13,257 women with BC), 58 were published since 2010. Women who were married, partnered, or in relationships represented 74.1% of the entire sample. Only a single study was conducted on women representing a sexual minority. We identified 22 assessment instruments and 40 sexual dysfunction (SdF) domains. The number of publications on SF in women with BC has increased in the last 10 years, but still remains low. Some groups of women are underrepresented, and some SdF domains are underdiagnosed, with the assessment instrument used affecting which domains are studied. Women with BC need to be better screened, as their quality of life (QoL) is affected by SdF.
Social Factors as Major Determinants of Rural Development Variation for Predicting Epidemic Vulnerability: A Lesson for the Future
Authors: Dudzińska Małgorzata , Gwiaździńska-Goraj Marta , Jezierska-Thöle Aleksandra
Published: October 2022

There have been changes in social attitudes in recent years. These changes have been a consequence of a new societal view of the common good, which manifests itself in social responsibility for a clean and healthy environment. The outbreak and spread of the COVID-19 epidemic has highlighted the socio-spatial variation across regions and countries. The epidemic necessitated restrictive measures by state authorities. In the initial period in many countries, the actions of the authorities were identical throughout the country. This was mainly due to a lack of information about the differentiation of areas in relation to the epidemic risk. The aim of the research was to present a model for classifying rural areas taking into account vulnerability to epidemic threats. The model takes into account demographic, social, economic and spatial-environmental development factors. A total of 33 indicators based on public statistics that can be used to determine the area’s vulnerability to epidemic threats were identified. The study showed that for Poland, 11 indicators are statistically significant to the developed classification model. The study found that social factors were vital in determining an area’s vulnerability to epidemic threats. We include factors such as average number of persons per one apartment, village centers (number), events (number), number of people per facility (cultural center, community center, club, community hall), residents of nursing homes per 1000 inhabitants, and the number of children in pre-school education establishments per 1000 children aged 3–5 years. The research area was rural areas in Poland. The results of the classification and the methods used should be made available as a resource for crisis management. This will enable a better response to threats from other epidemics in the future, and will influence the remodeling of the environment and social behavior to reduce risks at this risk, which has a significant impact on sustainable development in rural areas.
Community Assets for Health Model and Assessment Scale: A Delphi-Based Analysis and Expert Validation
Authors: Sáinz-Ruiz Pablo , Martínez-Riera José Ramón
Published: October 2022

The salutogenesis theory of Aaron Antonovsky and the Health Assets Model of Morgan and Ziglio have given rise to a notable interest in defining the resources available to individuals and the community to maintain or improve their health and well-being. The present study began by identifying the universal dimensions of Community Assets for Health, and then analyzed and validated an assessment scale following the Delphi method. A high degree of consensus was achieved among 13 experts from different disciplines. The results of the content analysis and statistical analysis led to a reconfiguring of an instrument that is so far unique in its approach. It is composed of 103 items across 14 dimensions (utility, intention, previous use, affordability, proximity, walkability, connectivity, intelligibility, identity, design, safety, diversity, public dimension, and sustainability).
High Frequency of Antibiotic Resistance Genes (ARGs) in the Lerma River Basin, Mexico
Authors: Tapia-Arreola Ana K. , Ruiz-Garcia Daniel A. , Rodulfo Hectorina , Sharma Ashutosh , De Donato Marcos
Published: October 2022

The spread of beta-lactamase-producing bacteria is of great concern and the environment has been found to be a main source of contamination. Herein, it was proposed to determine the frequency of antimicrobial-resistant-Gram-negative bacteria throughout the Lerma River basin using phenotypic and molecular methods. Resistant bacteria were isolated with chromogenic media and antimicrobial susceptibility tests were used to characterize their resistance. ARGs for beta-lactams, aminoglycosides, and quinolones were detected by PCR. Species were identified by Sanger sequencing the 16S rRNA gene and the representative genomes of MDR strains were sequenced by NGS. A high variation in the number of isolates was observed in the 20 sampled sites, while observing a low diversity among the resistant bacteria. Of the 12 identified bacterial groups, C. freundii, E. coli, and S. marcescens were more predominant. A high frequency of resistance to beta-lactams, quinolones, and aminoglycosides was evidenced, where the blaCTX,qnrB, qnrS y, and aac(6′)lb-cr genes were the most prevalent. C. freundii showed the highest frequency of MDR strains. Whole genome sequencing revealed that S. marcescens and K. pneumoniae showed a high number of shared virulence and antimicrobial resistance genes, while E. coli showed the highest number of unique genes. The contamination of the Lerma River with MDR strains carrying various ARGs should raise awareness among environmental authorities to assess the risks and regulations regarding the optimal hygienic and sanitary conditions for this important river that supports economic activities in the different communities in Mexico.
Organizational Factors and Their Impact on Mental Health in Public Safety Organizations
Authors: Edgelow Megan , Scholefield Emma , McPherson Matthew , Legassick Kathleen , Novecosky Jessica
Published: October 2022

Public safety personnel (PSP), including correctional officers, firefighters, paramedics, and police officers, have higher rates of mental health conditions than other types of workers. This scoping review maps the impact of organizational factors on PSP mental health, reviewing applicable English language primary studies from 2000–2021. JBI methodology for scoping reviews was followed. After screening, 97 primary studies remained for analysis. Police officers (n = 48) were the most frequent population studied. Correctional officers (n = 27) and paramedics (n = 27) were the second most frequently identified population, followed by career firefighters (n = 20). Lack of supervisor support was the most frequently cited negative organizational factor (n = 23), followed by negative workplace culture (n = 21), and lack of co-worker support (n = 14). Co-worker support (n = 10) was the most frequently identified positive organizational factor, followed by supervisor support (n = 8) and positive workplace culture (n = 5). This scoping review is the first to map organizational factors and their impact on PSP mental health across public safety organizations. The results of this review can inform discussions related to organizational factors, and their relationship to operational and personal factors, to assist in considering which factors are the most impactful on mental health, and which are most amenable to change.
Sodium Nitrite Intoxication and Death: Summarizing Evidence to Facilitate Diagnosis
Authors: Padovano Martina , Aromatario Mariarosaria , D’Errico Stefano , Concato Monica , Manetti Federico , David Maria Chiara , Scopetti Matteo , Frati Paola , Fineschi Vittorio
Published: October 2022

Background: Over the years, forensic pathology has registered the spread of new methods of suicide, such as the ingestion of sodium nitrite. Sodium nitrite causes increased methemoglobin, resulting in systemic hypoxia, metabolic acidosis, and cyanosis. Since sodium nitrite is a preservative, the ingestion of foods containing an excessive amount of this substance can also cause acute intoxication up to death. The present review is aimed at guiding health professionals in the identification and management of sodium-nitrite-related intoxications and deaths. Methods: A systematic literature search was carried out on PubMed by following the PRISMA statement’s criteria. A total of 35 studies with 132 cases were enrolled, and the data were cataloged in Microsoft Excel. To establish the causal correlation between sodium nitrite ingestion and death, the Naranjo Adverse Drug Reaction Probability Scale was used. Results: In addition to the small number of cases that have currently been published, the study demonstrated that there was a general methodological discrepancy in the diagnostic process. However, some interesting results have emerged, especially in post-mortem diagnostics. Conclusion: Sodium-nitrite-related deaths represent a challenge for forensic pathologists; therefore, it is important to promptly recognize the essential features and perform the necessary and unrepeatable examinations for the correct diagnosis of the cause of death.
Land Use Changes Influence the Soil Enzymatic Activity and Nutrient Status in the Polluted Taojia River Basin in Sub-Tropical China
Authors: Yuan Chenglin , Liang Siqi , Wu Xiaohong , Farooq Taimoor , Liu Tingting , Hu Yu , Wang Guangjun , Wang Jun , Yan Wende
Published: October 2022

Different land use practices may improve soil quality or lead to soil deterioration. Recently, environmental problems, such as heavy pollution and soil erosion, have led to serious land degradation in the Taojia River basin. In this study, we explored the soil fertility characteristics (mechanical composition; pH; soil organic matter (SOM); soil total nitrogen (TN); and the activity of four enzymes, i.e., urease, hydrogen peroxide, alkaline phosphatase, and sucrose enzymes) under different types of land use in the Taojia River basin. Soil samples were taken from 0–10 cm, 10–20 cm, and 20–40 cm depths from four different land use types that were widely used in the Taojia river basin, including cultivated land, vegetable fields, woodlands, and wastelands. The results showed that the soil enzyme activity and the constituents of the soil were closely related and significantly affected each other (p < 0.05). Woodland soil exhibited the highest content of SOM in all soil depths. Soil total nitrogen mainly depended on the accumulation of biomass and the decomposition intensity of organic matter, so the changes in TN followed the trends of the changes in SOM. Woodland soil showed an improved mechanical composition. We were also able to observe an increased clay content in woodland soil. Woodland soil also exhibited the reversal of soil desertification and an increase in nutrient/water retention capacity. Therefore, an increase in woodland areas would be an appropriate goal in terms of land use in order to improve the eco-environmental quality of the Taojia River basin.
Influence of Primary Palatal Surgery on Craniofacial Morphology in Patients with Cleft Palate Only (CPO)—Systematic Review with Meta-Analysis
Authors: Janiszewska-Olszowska Joanna , Grocholewicz Katarzyna , Mazur Marta , Jedliński Maciej
Published: October 2022

Background: Cleft palate only (CPO) is the second most prevalent cleft type. Both the cleft and palatal scarring may affect craniofacial growth. The aim of this systematic review was to summarize scientific evidence on effect of palatal surgery on craniofacial morphology in CPO. Methods: A search was conducted in PubMed, PMC, WoS, Scopus, Embase, using the keywords: “cleft palate” AND (“craniofacial morphology” OR “cephalometric analysis”) NOT “lip” with inclusion and exclusion criteria ensuring confident, direct comparison between study groups. The quality assessment was performed with Arrive’s scale for radiologic examinations. Results: Of 713 potential articles, 19 were subjected to qualitative analysis and 17 to meta-analysis, which confirmed reduced SNA in unoperated CPO versus non-cleft individuals. No scientific evidence was found directly assessing the effect of surgery on craniofacial morphology. The negative effect of palatal surgery was seen indirectly: in treated CPO versus non-cleft, the size effect of SNA is bigger than in untreated CPO versus non-cleft. A high heterogeneity came from a few non-European publications. Conclusions: CPO is associated with sagittal maxillary deficiency resulting both from the cleft and from primary surgery, disregarding cleft severity in operated CPO patients. Ethnic differences influence craniofacial morphology in CPO. This research received no external funding. Study protocol number in PROSPERO database: CRD42021268957.
Hemodialysis and Peritoneal Dialysis in Germany from a Health Economic View—A Propensity Score Matched Analysis
Authors: Shukri Arim , Mettang Thomas , Scheckel Benjamin , Schellartz Isabell , Simic Dusan , Scholten Nadine , Müller Martin , Stock Stephanie
Published: October 2022

Background: Hemodialysis (HD) and peritoneal dialysis (PD) are deemed medically equivalent for therapy of end-stage renal disease (ESRD) and reimbursed by the German statutory health insurance (SHI). However, although the home dialysis modality PD is associated with higher patient autonomy than HD, for unknown reasons, PD uptake is low in Germany. Hence, we compared HD with PD regarding health economic outcomes, particularly costs, as potentially relevant factors for the predominance of HD. Methods: Claims data from two German health insurance funds were analysed in a retrospective cohort study regarding the prevalence of HD and PD in 2013–2016. Propensity score matching created comparable HD and PD groups (n = 436 each). Direct annual health care costs were compared. A sensitivity analysis included a comparison of different matching techniques and consideration of transportation costs. Additionally, hospitalisation and survival were investigated using Poisson regression and Kaplan-Meier curves. Results: Total direct annual average costs were higher for HD (€47,501) than for PD (€46,235), but not significantly (p = 0.557). The additional consideration of transportation costs revealed an annual cost advantage of €7000 for PD. HD and PD differed non-significantly in terms of hospitalisation and survival rates (p = 0.610/p = 0.207). Conclusions: PD has a slight non-significant cost advantage over HD, especially when considering transportation costs.
To Vaccinate or Not to Vaccinate—Reasons of Willingness and Reluctance of Students against SARS-CoV-2 Vaccination—An International Experience
Authors: Sitarz Ryszard , Forma Alicja , Karakuła Kaja , Juchnowicz Dariusz , Baj Jacek , Bogucki Jacek , Rog Joanna , Tee Michael L. , Tee Cherica A. , Ly-Uson Josefina T. , Islam Md. Saiful , Sikder Md. Tajuddin , El-Monshed Ahmed Hashem , Loutfy Ahmed , Qureshi Muhammad Fazal Hussain , Abbas Munib , Taseen Shafaq , Lakhani Mahira , Wang Cuiyan , Wan Xiaoyang , Tan Yilin , Pan Riyu , Ho Roger , Jayakumar Saikarthik , Ilango Saraswathi , Kumar Senthil , Ruiz-Chow Ángel A. , Iturbide Adriana , González-Mille David D. , Doan Linh Phuong , Karakuła-Juchnowicz Hanna
Published: October 2022

Despite the vaccine against the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) being reported to be safe and effective, the unwillingness to vaccinate and doubts are still common. The aim of this international study was to assess the major reasons for the unwillingness to vaccinate in a group of students from Poland (n = 1202), Bangladesh (n = 1586), India (n = 484), Mexico (n = 234), Egypt (n = 566), Philippines (n = 2076), Pakistan (n = 506), Vietnam (n = 98) and China (n = 503). We conducted an online cross-sectional study that aimed to assess (1) the percentage of vaccinated and unvaccinated students and (2) the reasons associated with willingness/unwillingness to the vaccine. The study included 7255 respondents from 9 countries with a mean age of 21.85 ± 3.66 years. Only 22.11% (n = 1604) of students were vaccinated. However, the majority (69.25%, n = 5025) expressed a willingness to be vaccinated. More willing to vaccinate were students in informal relationships who worked mentally, used psychological/psychiatric services before the pandemic, and studied medicine. There are cultural differences regarding the reasons associated with the unwillingness to vaccinate, but some ‘universal’ might be distinguished that apply to the whole group.
Map-Matching Error Identification in the Absence of Ground Truth
Authors: Dey Subhrasankha , Tomko Martin , Winter Stephan
Published: October 2022

Map-matching of trajectory data has widespread applications in vehicle tracking, traffic flow analysis, route planning, and intelligent transportation systems. Map-matching algorithms snap a set of trajectory points observed by a satellite navigation system to the most likely route segments of a map. However, due to the unavoidable errors in the recorded trajectory points and the incomplete map data, map-matching algorithms may match points to incorrect segments, leading to map-matching errors. Identification of these map-matching errors in the absence of ground truth can only be achieved by visual inspection and reasoning. Thus, the identification of map-matching errors without ground truth is a time-consuming and mundane task. Although research has focused on improving map-matching algorithms, to our knowledge no attempts have been made to automatically classify and identify the residual map-matching errors. In this work, we propose the first method to automatically identify map-matching errors in the absence of ground truth, i.e., only using the recorded trajectory points and the map-matched route. We have evaluated our method on a public dataset and observed an average accuracy of 91% in automatically identifying map-matching errors, thus helping analysts to significantly reduce manual effort for map-matching quality assurance.
Improved Locomotor Recovery in a Rat Model of Spinal Cord Injury by BioLuminescent-OptoGenetic (BL-OG) Stimulation with an Enhanced Luminopsin
Authors: Ikefuama Ebenezer C. , Kendziorski Griffin E. , Anderson Kevin , Shafau Lateef , Prakash Mansi , Hochgeschwender Ute , Petersen Eric D.
Published: October 2022

Irrespective of the many strategies focused on dealing with spinal cord injury (SCI), there is still no way to restore motor function efficiently or an adequate regenerative therapy. One promising method that could potentially prove highly beneficial for rehabilitation in patients is to re-engage specific neuronal populations of the spinal cord following SCI. Targeted activation may maintain and strengthen existing neuronal connections and/or facilitate the reorganization and development of new connections. BioLuminescent-OptoGenetics (BL-OG) presents an avenue to non-invasively and specifically stimulate neurons; genetically targeted neurons express luminopsins (LMOs), light-emitting luciferases tethered to light-sensitive channelrhodopsins that are activated by adding the luciferase substrate coelenterazine (CTZ). This approach employs ion channels for current conduction while activating the channels through treatment with the small molecule CTZ, thus allowing non-invasive stimulation of all targeted neurons. We previously showed the efficacy of this approach for improving locomotor recovery following severe spinal cord contusion injury in rats expressing the excitatory luminopsin 3 (LMO3) under control of a pan-neuronal and motor-neuron-specific promoter with CTZ applied through a lateral ventricle cannula. The goal of the present study was to test a new generation of LMOs based on opsins with higher light sensitivity which will allow for peripheral delivery of the CTZ. In this construct, the slow-burn Gaussia luciferase variant (sbGLuc) is fused to the opsin CheRiff, creating LMO3.2. Taking advantage of the high light sensitivity of this opsin, we stimulated transduced lumbar neurons after thoracic SCI by intraperitoneal application of CTZ, allowing for a less invasive treatment. The efficacy of this non-invasive BioLuminescent-OptoGenetic approach was confirmed by improved locomotor function. This study demonstrates that peripheral delivery of the luciferin CTZ can be used to activate LMOs expressed in spinal cord neurons that employ an opsin with increased light sensitivity.
PRG3 and PRG5 C-Termini: Important Players in Early Neuronal Differentiation
Authors: Brandt Nicola , Willmer Jan Philipp , Ayon-Olivas Maurilyn S. , Banicka Veronika , Witt Martin , Wree Andreas , Groß Isabel , Gläser Anne , Hausmann Jens , Bräuer Anja U.
Published: October 2022

The functional importance of neuronal differentiation of the transmembrane proteins’ plasticity-related genes 3 (PRG3) and 5 (PRG5) has been shown. Although their sequence is closely related, they promote different morphological changes in neurons. PRG3 was shown to promote neuritogenesis in primary neurons; PRG5 contributes to spine induction in immature neurons and the regulation of spine density and morphology in mature neurons. Both exhibit intracellularly located C-termini of less than 50 amino acids. Varying C-termini suggested that these domains shape neuronal morphology differently. We generated mutant EGFP-fusion proteins in which the C-termini were either swapped between PRG3 and PRG5, deleted, or fused to another family member, plasticity-related gene 4 (PRG4), that was recently shown to be expressed in different brain regions. We subsequently analyzed the influence of overexpression in immature neurons. Our results point to a critical role of the PRG3 and PRG5 C-termini in shaping early neuronal morphology. However, the results suggest that the C-terminus alone might not be sufficient for promoting the morphological effects induced by PRG3 and PRG5.
Structural Insight into TNIK Inhibition
Authors: Kukimoto-Niino Mutsuko , Shirouzu Mikako , Yamada Tesshi
Published: October 2022

TRAF2- and NCK-interacting kinase (TNIK) has emerged as a promising therapeutic target for colorectal cancer because of its essential role in regulating the Wnt/β-catenin signaling pathway. Colorectal cancers contain many mutations in the Wnt/β-catenin signaling pathway genes upstream of TNIK, such as the adenomatous polyposis coli (APC) tumor suppressor gene. TNIK is a regulatory component of the transcriptional complex composed of β-catenin and T-cell factor 4 (TCF4). Inhibition of TNIK is expected to block the aberrant Wnt/β-catenin signaling caused by colorectal cancer mutations. Here we present structural insights into TNIK inhibitors targeting the ATP-binding site. We will discuss the effects of the binding of different chemical scaffolds of nanomolar inhibitors on the structure and function of TNIK.
Endoplasmic Reticulum Stress Underlies Nanosilver-Induced Neurotoxicity in Immature Rat Brain
Authors: Dąbrowska-Bouta Beata , Sulkowski Grzegorz , Gewartowska Magdalena , Strużyńska Lidia
Published: October 2022

The growing production of silver nanoparticles (AgNPs), and their widespread use in medical and consumer products, poses a potential threat to the environment and raises questions about biosafety. Immature organisms are particularly susceptible to various insults during development. The biological characteristics of immature organisms are different from those of adults, and dictate the consequences of exposure to various toxic substances, including AgNPs. Nanoparticles are highly reactive and can easily cross the blood–brain barrier (BBB) to accumulate in brain tissues. It is therefore important to investigate the molecular mechanisms of AgNP-induced neurotoxicity in the developing brain. Immature 2-week-old rats were exposed to a low dose of AgNPs (0.2 mg/kg b.w.) over a long period. Subsequently, brain tissues of the animals were subjected to ultrastructural and molecular analyses to determine endoplasmic reticulum (ER) stress. Ultrastructural markers of ER stress, such as pathological alterations in the ER and elongated forms of mitochondria accompanied by autophagy structures, were confirmed to be present in AgNP-exposed rat brain. Evidence for induction of ER stress in neurons was also provided by molecular markers. Upregulation of genes related to the ER-stress-induced unfolded protein response (UPR) pathway, such as GRP78, PERK, and CHOP ATF-6, was observed at the transcriptional and translational levels. The results show that prolonged exposure of immature rats to a low dose of AgNPs during the developmental period leads to induction of ER stress in the neurons of the developing brain. Simultaneously, in response to AgNP-induced ER stress, neurons promote protective mechanisms that partially compensate for ER stress by regulating the biodynamic processes of mitochondria and autophagy.
Puerarin-V Improve Mitochondrial Respiration and Cardiac Function in a Rat Model of Diabetic Cardiomyopathy via Inhibiting Pyroptosis Pathway through P2X7 Receptors
Authors: Sun Shuchan , Dawuti Awaguli , Gong Difei , Wang Ranran , Yuan Tianyi , Wang Shoubao , Xing Cheng , Lu Yang , Du Guanhua , Fang Lianghua
Published: October 2022

There is a new form of puerarin, puerarin-V, that has recently been developed, and it is unclear whether puerarin-V has a cardioprotective effect on diabetic cardiomyopathy (DCM). Here, we determined whether puerarin-V had any beneficial influence on the pathophysiology of DCM and explored its possible mechanisms. By injecting 30 mg/kg of STZ intraperitoneally, diabetes was induced in rats. After a week of stability, the rats were injected subcutaneously with ISO (5 mg/kg). We randomly assigned the rats to eight groups: (1) control; (2) model; (3) metformin; (4–6) puerarin-V at different doses; (7) puerarin (API); (8) puerarin injection. DCM rats were found to have severe cardiac insufficiency (arrythmia, decreased LVdP/dt, and increased E/A ratio). In addition, cardiac injury biomarkers (cTn-T, NT-proBNP, AST, LDH, and CK-MB), inflammatory cytokines (IL-1β, IL-18, IL-6, and TNF-α), and oxidative damage markers (MDA, SOD and GSH) were markedly increased. Treatment with puerarin-V positively adjusts these parameters mentioned above by improving cardiac function and mitochondrial respiration, suppressing myocardial inflammation, and maintaining the structural integrity of the cardiac muscle. Moreover, treatment with puerarin-V inhibits the P2X7 receptor-mediated pyroptosis pathway that was upregulated in diabetic hearts. Given these results, the current study lends credence to the idea that puerarin-V can reduce myocardial damage in DCM rats. Furthermore, it was found that the effect of puerarin-V in diabetic cardiomyopathy is better than the API, the puerarin injection, and metformin. Collectively, our research provides a new therapeutic option for the treatment of DCM in clinic.
Panobinostat Synergistically Enhances the Cytotoxicity of Microtubule Destabilizing Drugs in Ovarian Cancer Cells
Authors: Ovejero-Sánchez María , Asensio-Juárez Gloria , González Myriam , Puebla Pilar , Vicente-Manzanares Miguel , Pélaez Rafael , González-Sarmiento Rogelio , Herrero Ana Belén
Published: October 2022

Ovarian cancer (OC) is one of the most common gynecologic neoplasia and has the highest mortality rate, which is mainly due to late-stage diagnosis and chemotherapy resistance. There is an urgent need to explore new and better therapeutic strategies. We have previously described a family of Microtubule Destabilizing Sulfonamides (MDS) that does not trigger multidrug-mediated resistance in OC cell lines. MDS bind to the colchicine site of tubulin, disrupting the microtubule network and causing antiproliferative and cytotoxic effects. In this work, a novel microtubule-destabilizing agent (PILA9) was synthetized and characterized. This compound also inhibited OC cell proliferation and induced G2/M cell cycle arrest and apoptosis. Interestingly, PILA9 was significantly more cytotoxic than MDS. Here, we also analyzed the effect of these microtubule-destabilizing agents (MDA) in combination with Panobinostat, a pan-histone deacetylase inhibitor. We found that Panobinostat synergistically enhanced MDA-cytotoxicity. Mechanistically, we observed that Panobinostat and MDA induced α-tubulin acetylation and that the combination of both agents enhanced this effect, which could be related to the observed synergy. Altogether, our results suggest that MDA/Panobinostat combinations could represent new therapeutic strategies against OC.
Dysregulation of miR-1-3p: An Early Event in Colitis-Associated Dysplasia
Authors: Fragoso Mariana F. , Fernandez Geysson J. , Vanderveer Lisa , Cooper Harry S. , Slifker Michael , Clapper Margie L.
Published: October 2022

Detection of colorectal dysplasia during surveillance colonoscopy remains the best method of determining risk for colitis-associated colorectal cancer (CAC). miRNAs (miRs) show great promise as tissue-specific biomarkers of neoplasia. The goal of this study was to explore the miR expression profile of precancerous dysplastic lesions in the AOM/DSS mouse model and identify early molecular changes associated with CAC. Epithelial cells were laser-microdissected from the colonic mucosa (inflamed versus dysplastic) of mice with AOM/DSS-induced colitis. A miR signature that can distinguish inflamed non-neoplastic mucosa from dysplasia was identified. Bioinformatic analyses led to the discovery of associated miR gene targets and enriched pathways and supported the construction of a network interaction map. miR-1a-3p was one of the miRs with the highest number of predicted targets, including Cdk6. Interestingly, miR-1a-3p and Cdk6 were down- and up-regulated in dysplastic lesions, respectively. Transfection of HCT116 and RKO cells with miR-1a-3p mimics induced apoptosis and cell cycle arrest in G1, suggesting its biological function. A slight reduction in the level of CDK6 transcripts was also observed in cells transfected with miR-1. These data provide novel insight into the early molecular alterations that accompany the development of CAC and identify a miR signature that represents a promising biomarker for the early detection of colitis-associated dysplasia.