bluuebunny/crossref_metadata_2025 huggingface dataset:
Severe Fever with Thrombocytopenia Syndrome Virus in Ticks in the Republic of Korea
Authors: Kang Jun-Gu , Cho Yoon-Kyoung , Jo Young-Sun , Han Sun-Woo , Chae Jeong-Byoung , Park Jung-Eun , Jeong Hyesung , Jheong Weon-Hwa , Chae Joon-Seok
Published: February 2022

Severe fever with thrombocytopenia syndrome virus (SFTSV) is a zoonotic, tick-borne RNA virus of the genus Bandavirus (Family Phenuiviridae), mainly reported in China, Japan, and the Republic of Korea (Korea). For the purpose of this study, a total of 3,898 adult and nymphal ticks of species Haemaphysalis longicornis (94.2%), Haemaphysalis flava (5.0%), Ixodes nipponensis (0.8%), and 1 specimen of Ixodes ovatus, were collected from the Deogyusan National Park, Korea, between April 2016 and June 2018. A single-step reverse transcriptase–nested PCR was performed, targeting the S segment of the SFTSV RNA. Total infection rate (IR) of SFTSV in individual ticks was found to be 6.0%. Based on developmental stages, IR was 5.3% in adults and 6.0% in nymphs. The S segment sequences obtained from PCR were divided into 17 haplotypes. All haplotypes were phylogenetically clustered into clades B-2 and B-3, with 92.7% sequences in B-2 and 7.3% in B-3. These observations indicate that the Korean SFTSV strains were closer to the Japanese than the Chinese strains. Further epidemiological studies are necessary to better understand the characteristics of the Korean SFTSV and its transmission cycle in the ecosystem.
Characterization of a Peptide Antibody Specific to the Adenylyl Cyclase-Associated Protein of Acanthamoeba castellanii
Authors: Kim Min-Jeong , Lee Hae-Ahm , Quan Fu-Shi , Kong Hyun-Hee , Moon Eun-Kyung
Published: February 2022

Acanthamoeba keratitis (AK) is a rare infectious disease and accurate diagnosis has remained arduous as clinical manifestations of AK were similar to keratitis of viral, bacterial, or fungal origins. In this study, we described the production of a polyclonal peptide antibody against the adenylyl cyclase-associated protein (ACAP) of A. castellanii, and evaluated its differential diagnostic potential. Enzyme-linked immunosorbent assay revealed high titers of A. castellanii-specific IgG and IgA antibodies being present in low dilutions of immunized rabbit serum. Western blot analysis revealed that the ACAP antibody specifically interacted with A. castellanii, while not interacting with human corneal epithelial (HCE) cells and other causes of keratitis such as Fusarium solani, Pseudomonas aeruginosa, and Staphylococcus aureus. Immunocytochemistry (ICC) results confirmed the specific detection of trophozoites and cysts of A. castellanii co-cultured with HCE cells. The ACAP antibody also specifically interacted with the trophozoites and cysts of 5 other Acanthamoeba species. These results indicate that the ACAP antibody of A. castellanii can specifically detect multiple AK-causing members belonging to the genus Acanthamoeba and may be useful for differentially diagnosing Acanthamoeba infections.
Feasibility Study of Biogas Production and Applications using Rural Biomass in Northern Queensland, Australia.
Authors: Ansari Ammar Arif , Elshamy Yousef , Dheen Manush , Khalid Ahmed , Correia Cavin Hansel , Talal Ribal , Doucet Florian
Published: February 2022

Cape York, in Northern Queensland, Australia, is a remote area with a limited supply of energy, making it very hard for the locals to have an easier life. Locals use diesel as a fuel for transportation which is economically harmful to the environment due to the carbon emissions. The research team found a solution for these problems and decided to design a biogas plant, which will be environmentally friendly, cost-effective, and will produce job opportunities for the locals. The plant aims to produce biogas from the raw manure of the cattle from the farms in Cairns, a nearby neighborhood. The long-term plan of the plant is to be sustainable and profitable. The business plan predicts the break-even point of the business as 4 months and the payback period as less than a year.
Human Growth Factor/Immunoglobulin Complexes for Treatment of Myocardial Ischemia-Reperfusion Injury
Authors: Liebman Benjamin , Schwaegler Claire , Foote Andrea T. , Rao Krithika S. , Marquis Taylor , Aronshtam Alexander , Bell Stephen P. , Gogo Prospero , LaChapelle Richard R. , Spees Jeffrey L.
Published: February 2022

Hepatocyte Growth Factor (HGF) and Fibroblast Growth Factor 2 (FGF2) are receptor tyrosine kinase agonists that promote cell survival after tissue injury and angiogenesis, cell proliferation and migration during tissue repair and regeneration. Both ligands have potential as systemic treatments for ischemia-reperfusion injury, however clinical use of HGF and FGF2 has been limited by poor pharmacokinetic profiles, i.e., their susceptibility to serum proteases, rapid clearance and short half-lives. Previously, we reported vaso- and cardioprotective protein complexes formed between HGF and polyclonal, non-specific immunoglobulin (IgG) with therapeutic efficacy in a rat model of myocardial ischemia with reperfusion (MI/R). Here, using a pre-clinical porcine MI/R model, we demonstrate human HGF/IgG complexes provide significant myocardial salvage, reduce infarct size, and are detectable in myocardial tissue 24 h after intracoronary injection. Furthermore, we show that multiple daily infusions of HGF/IgG complexes after MI do not lead to production of HGF-specific auto-antibodies, an important concern for administered biologic drugs. In experiments to identify other growth factors that non-covalently interact with IgG, we found that human FGF2 associates with IgG. Similar to human HGF/IgG complexes, FGF2/IgG complexes protected primary human cardiac endothelial cells under simulated ischemia (1% oxygen and nutrient deprivation) for 48–72 h. Molecular modeling studies suggested that FGF2 and HGF both interact with the Fc domain of IgG. Also, we tested whether an Fc-fusion protein would bind FGF2 to form complexes. By native gel electrophoretic assays and biochemical pulldowns, we found that Jagged1, a Notch1 ligand that controls stem cell self-renewal and tissue regeneration, bound FGF2 when presented as a Jagged1- Fc fusion protein. Our results suggest that human growth factor/IgG and FGF2/Fc- fusion complexes have potential to provide a biologics platform to treat myocardial ischemia-reperfusion and other forms of tissue injury.
VHPKQHR Peptide Modified Ultrasmall Paramagnetic Iron Oxide Nanoparticles Targeting Rheumatoid Arthritis for T1-Weighted Magnetic Resonance Imaging
Authors: Zhang Chunyu , Huang Wentao , Huang Chen , Zhou Chengqian , Tang Yukuan , Wei Wei , Li Yongsheng , Tang Yukuan , Luo Yu , Zhou Quan , Chen Wenli
Published: February 2022

Magnetic resonance imaging (MRI) could be the ideal diagnostic modality for early rheumatoid arthritis (RA). Vascular cell adhesion molecule-1 (VCAM-1) is highly expressed in synovial locations in patients with RA, which could be a potential target protein for RA diagnosis. The peptide VHPKQHR (VHP) has a high affinity to VCAM-1. To make the contrast agent to target RA at an early stage, we used VHP and ultrasmall paramagnetic iron oxide (USPIO) to synthesize UVHP (U stands for USPIO) through a chemical reaction with 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide. The size of UVHP was 6.7 nm; the potential was −27.7 mV, and ther2/r1value was 1.73. Cytotoxicity assay exhibited that the cell survival rate was higher than 80% at even high concentrations of UVHP (Fe concentration 200 µg/mL), which showed the UVHP has low toxicity. Compared with no TNF-α stimulation, VCAM-1 expression was increased nearly 3-fold when mouse aortic endothelial cells (MAECs) were stimulated with 50 ng/mL TNF-α; cellular Fe uptake was increased very significantly with increasing UVHP concentration under TNF-α treatment; cellular Fe content was 17 times higher under UVHP with Fe concentration 200 µg/mL treating MAECs. These results indicate that UVHP can target overexpression of VCAM-1 at the cellular level. RA mice models were constructed with adjuvant-induced arthritis.In vivoMRI and biodistribution results show that the signal intensity of knee joints was increased significantly and Fe accumulation in RA model mice compared with normal wild-type mice after injecting UVHP 24 h. These results suggest that we have synthesized a simple, low-cost, and less toxic contrast agent UVHP, which targeted VCAM-1 for early-stage RA diagnosis and generates high contrast in T1-weighted MRI.
Postoperative Hip Center Position Associated With the Range of Internal Rotation and Extension During Gait in Hip Dysplasia Patients After Total Hip Arthroplasty
Authors: Hu Yi , Zou Diyang , Sun Qi , Jiang Mengda , Li Huiwu , Tsai Tsung-Yuan , Zhang Jingwei
Published: February 2022

Background: Total hip arthroplasty (THA) for hip dysplasia patients is sometimes complex and compromises pathomorphological changes in these patients. However, it remains unclear whether it is preoperative deformities or postoperative structures or anatomic changes during THA that have the most remarkable correlation with the hip dynamic function during gait. The purpose of this study was to investigate this relationship and propose insights into the surgical reconstruction strategy in patients with developmental dysplasia of the hip.

Methods: A total of 21 unilateral hip dysplasia patients received computed tomography scans for the creation of 3D hip models before surgery and at the last follow-up. Acetabular and femoral orientations, hip center positions, and femoral length were measured before and after THA. Hip kinematics of the operated side during gait was quantified using a dual fluoroscopic imaging technique. Pearson correlation and multiple linear regression were performed to evaluate the relationship between hip maximum range of motion in six directions and demographics characters and above hip anatomic parameters before and after THA and their changes in surgery.

Results: Pearson correlation analysis found significant correlations with the gait range of motion mainly in postoperative structures, including postoperative hip center positions and acetabulum and combined anteversion. Further multiple linear regression indicated that a laterally placed hip center was significantly correlated with an increased internal rotation (R2 = 0.25, p = 0.021), which together with increased postoperative acetabulum anteversion explained 45% of external rotation decreasing (p = 0.004). A proximally placed hip center was correlated with more extension (R2 = 0.30, p = 0.010). No significant demographic characters or preoperative deformities or surgical changes were included into other multiple regression models.

Conclusion: Strong correlations between postoperative structures, especially hip center positions and gait range of motion in unilateral hip dysplasia patients after THA were found. It indicated that postoperative prosthesis structures, particularly hip center positions had significant impact on the hip gait motion range and should be treated with particular caution in surgery.
A Novel Signature of Lipid Metabolism-Related Gene Predicts Prognosis and Response to Immunotherapy in Lung Adenocarcinoma
Authors: Zhang Kai , Qian Ying , Quan Xiaowei , Zhu Tengteng , Qian Biyun
Published: February 2022

Background: Lipid metabolism disorder, a new hallmark of cancer initiation, has been involved in lung adenocarcinoma (LUAD). However, few biomarkers about lipid metabolism-related genes (LMRGs) have been developed for prognosis prediction and clinical treatment of LUAD patients.

Methods: In this study, we constructed and validated an effective prognostic prediction model for LUAD patients depending on LMRGs. Subsequently, we investigated the prediction model from immune microenvironment, genomic changes, and immunotherapy.

Results: Then, eleven LMRGs were identified and applied to LUAD subtyping. In comparison with the high-risk group, the low-risk group exhibited a remarkably favorable prognosis, along with a higher immune score and lower tumor purity. Moreover, the low-risk group presented higher levels of immune checkpoint molecules, lower tumor immune dysfunction and exclusion (TIDE) score and tumor mutation burden (TMB), and higher likelihood of benefiting from immunotherapy. Furthermore, the genomic changes of six LMRGs (CD79A, HACD1, CYP17A1, SLCO1B3, ANGPTL4, and LDHA) were responsible for the difference in susceptibility to LUAD by greatly influencing B-cell activation.

Conclusion: Generally speaking, the LMRG model is a reliable independent biomarker for predicting adverse outcomes in LUAD patients and has the potential to facilitate risk-stratified immunotherapy.
A Bioelectrochemical System for Waste Degradation and Energy Recovery From Industrial Coffee Wastewater
Authors: Agudelo-Escobar Lina María , Cabrera Santiago Erazo , Avignone Rossa Claudio
Published: February 2022

The primary production of coffee involves the extensive use of water resources, since it is not only used for irrigation of coffee plantations, but it is also required in large volumes for the processing of the coffee berry to obtain high quality green beans. It is calculated that for every kg of dry coffee grain produced, up to 40 L of water are consumed, and its disposal represents a significant environmental problem, since most coffee growers are small producers with no access to efficient technologies for wastewater treatment. This situation leads to these liquid wastes to be discarded untreated in natural water sources, generating environmental pollution and public health problems. Bioelectrochemical Systems (BES) have been proposed as an alternative to conventional wastewater treatments, either as a primary bioremediation strategy or for secondary wastewater treatment systems. Among BES, microbial fuel cells (MFCs) are designed to exploit the metabolic capability of andophilic microorganisms to degrade the organic matter present in the waste. Anodophilic microorganisms use electrodes as terminal electron acceptors, generating a flow of electrons that can be used in the generation of electricity. In this work, we evaluated the ability of native microbial communities to degrade the organic matter present in wastewater from the coffee agroindustry and its electrogenic potential for the co-generation of electricity was evaluated using an MFC device developed by the authors. Wastewater samples obtained at different stages of the coffee wet process were used as inoculum and feedstocks. The system was operated in fed-batch, in both open and closed-circuit conditions, for 60 days. The degree of decontamination or bioremediation of the wastewater was assessed by measurements of physicochemical parameters. For the characterization of the native microbial community, microscopic and molecular techniques were used and the electrogenic potential was established by assessing the electrochemical performance of the system. With the proposed bioelectrochemical system, a reduction of up to 70% of the initial content of organic matter of the residual water from the coffee benefit was achieved, and open circuit voltages of up to 400 mV were recorded, comparable to those reported for conventional air breathing cathode MFC.
IBX-Mediated Organic Transformations in Heterocyclic Chemistry-A Decade Update
Authors: Venkata Durga Nageswar Yadavalli , Ramesh Katla , Rakhi Katla
Published: February 2022

O-Iodoxybenzoic acid (IBX) is a very mild and efficient hypervalent iodine synthetic reagent useful to carry out several selective oxidations. The present review highlights research reports on IBX-assisted transformations in heterocyclic derivatives, particularly from 2010 onward.
Facile Synthesis of Water-Soluble Rhodamine-Based Polymeric Chemosensors via Schiff Base Reaction for Fe3+ Detection and Living Cell Imaging
Authors: Qiu Xiaoyong , Huang Jun , Wang Ning , Zhao Kaijie , Cui Jiwei , Hao Jingcheng
Published: February 2022

Quantitative and accurate determination of iron ions play a vital role in maintaining environment and human health, but very few polymeric chemosensors were available for the detection of Fe3+ in aqueous solutions. Herein, a water-soluble rhodamine-poly (ethylene glycol) conjugate (DRF-PEG), as a dual responsive colorimetric and fluorescent polymeric sensor for Fe3+ detection with high biocompatibility, was first synthesized through Schiff base reaction between rhodamine 6G hydrazide and benzaldehyde-functionalized polyethylene glycol. As expected, the introduction of PEG segment in DRF-PEG significantly improved the water solubility of rhodamine derivatives and resulted in a good biosensing performance. The detection limit of DRF-PEG for Fe3+ in pure water is 1.00 μM as a fluorescent sensor and 3.16 μM as a colorimetric sensor at pH 6.5. The specific sensing mechanism of DRF-PEG toward Fe3+ is proposed based on the intramolecular charge transfer (ICT) mechanism, in which the O and N atoms in rhodamine moiety, together with the benzene groups from benzaldehyde-modified PEG segment, participate in coordination with Fe3+. Furthermore, DRF-PEG was applied for the ratiometric imaging of Fe3+ in HeLa cells and showed the potential for quantitative determination of Fe3+ in fetal bovine serum samples. This work provides insights for the design of water-soluble chemosensors, which can be implemented in iron-related biological sensing and clinical diagnosis.
Cationic Metal–Organic Framework-Based Mixed-Matrix Membranes for Fast Sensing and Removal of Cr2O72− Within Water
Authors: Zhang Shuyun , Zheng Heqi , Yang Yu , Qian Guodong , Cui Yuanjing
Published: February 2022

Considering that metal–organic framework (MOF)-polymer mixed-matrix membranes (MMMs) can overcome the drawbacks of intrinsic fragility and poor processability of pure-MOF membranes, we designed MOF-based MMMs for efficient removal and fast fluorescence sensing of heavily toxic ions within water systems simultaneously. In this work, a series of MOF-based MMMs are prepared by mixing a hydrolytically stable cationic [Eu7 (mtb)5(H2O)16]·NO3 8DMA·18H2O (denoted as Eu-mtb) MOF material into poly (vinylidene fluoride) with high loadings up to 70%. The free volume at the interface between the polymer and Eu-mtb particles, combined with the permanent porosity and uniform distribution of Eu-mtb particles, enables these MMMs to show fast enrichment of Cr2O72- from solutions and consequently have a full contact between the analyte and MOFs. The developed Eu-mtb MMM (70wt% loading) thus shows both efficient removal and exceptional fluorescence sensing of Cr2O72- in aqueous media. The overall adsorption capacity of the Eu-mtb MMM (70 wt% loading) for Cr2O72- reaches up to 33.34 mg/g, which is 3.4 times that of powder-form Eu-mtb. The detection limit of the Eu-mtb MMM (70 wt% loading) for Cr2O72- is around 5.73 nM, which is lower than that of the reported powder-form Eu-mtb. This work demonstrates that it is feasible to develop flexible luminescent MOF-based MMMs as a significant platform for efficient removal and sensitive sensing of pollutants from water systems simultaneously.
Influence of Fluoride-Resistant Streptococcus mutans Within Antagonistic Dual-Species Biofilms Under Fluoride In Vitro
Authors: Zhang Keke , Xiang Yangfan , Peng Youjian , Tang Fengyu , Cao Yanfan , Xing Zhenjie , Li Yejian , Liao Xiangyan , Sun Yan , He Yan , Ye Qingsong
Published: February 2022

The widespread application of fluoride, an extremely effective caries prevention agent, induces the generation of fluoride-resistant strains of opportunistic cariogenic bacteria such as fluoride-resistant Streptococcus mutans (S. mutans). However, the influence of this fluoride-resistant strain on oral microecological homeostasis under fluoride remains unknown. In this study, an antagonistic dual-species biofilm model composed of S. mutans and Streptococcus sanguinis (S. sanguinis) was used to investigate the influence of fluoride-resistant S. mutans on dual-species biofilm formation and pre-formed biofilms under fluoride to further elucidate whether fluoride-resistant strains would influence the anti-caries effect of fluoride from the point of biofilm control. The ratio of bacteria within dual-species biofilms was investigated using quantitative real-time PCR and fluorescence in situ hybridization. Cristal violet staining, scanning electron microscopy imaging, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assay were used to evaluate biofilm biomass, biofilm structure, and metabolic activity, respectively. Biofilm acidogenicity was determined using lactic acid and pH measurements. The anthrone method and exopolysaccharide (EPS) staining were used to study the EPS production of biofilms. We found that, in biofilm formation, fluoride-resistant S. mutans occupied an overwhelming advantage in dual-species biofilms under fluoride, thus showing more biofilm biomass, more robust biofilm structure, and stronger metabolic activity (except for 0.275 g/L sodium fluoride [NaF]), EPS production, and acidogenicity within dual-species biofilms. However, in pre-formed biofilms, the advantage of fluoride-resistant S. mutans could not be fully highlighted for biofilm formation. Therefore, fluoride-resistant S. mutans could influence the anti-caries effect of fluoride on antagonistic dual-species biofilm formation while being heavily discounted in pre-formed biofilms from the perspective of biofilm control.
Efficacy of Fecal Microbiota Transplantation in Irritable Bowel Syndrome: A Meta-Analysis of Randomized Controlled Trials
Authors: Wu Jie , Lv Liang , Wang Chunlian
Published: February 2022

Background

Randomized controlled trials (RCTs) have examined the efficacy of fecal microbiota transplantation (FMT) in irritable bowel syndrome (IBS) with inconsistent results. We performed a meta-analysis to assess both the short- and long-term efficacy of FMT in IBS.

Methods

MEDLINE, EMBASE, and the Cochrane Central Register were searched through September 2021. RCTs recruiting adult patients with IBS that compared FMT with placebo with dichotomous data of response to therapy were eligible. Dichotomous data were pooled to obtain a relative risk (RR) of symptom not improving after therapy. RR was also pooled for adverse events (AEs). Continuous data were calculated using a mean difference for IBS-Quality of Life (IBS-QoL). GRADE methodology was used to assess quality of evidence.

Results

The search strategy generated 658 citations. Seven RCTs comprising 472 patients with IBS were included. FMT was not associated with a significant improvement in global symptom in IBS at 12 weeks in comparison with placebo (RR 0.75, 95% CI 0.43–1.31) with high heterogeneity between studies (I2 87%). Subgroup analyses showed that FMT was superior to placebo when administered via colonoscopy or gastroscope (RR 0.70, 95% CI 0.51–0.96; RR 0.37, 95% CI 0.14–0.99, respectively, while FMT was inferior to placebo when administered via oral capsules (RR 1.88, 95% CI 1.06–3.35). FMT induced a significant improvement in IBS-QoL compared to placebo (mean difference 9.39, 95% CI 3.86–14.91) at 12 weeks. No significant difference in the total number of AEs was observed between FMT and placebo (RR 1.20, 95% CI 0.59–2.47). FMT did not significantly improve global symptom in IBS at 1-year follow-up compared with placebo (RR 0.90, 95% CI 0.72–1.12). The GRADE quality evidence to support recommending FMT in IBS was very low.

Conclusion

IBS patients may benefit from FMT when administered via colonoscopy or gastroscope. FMT may improve the quality of life of IBS. The long-term use of FMT in IBS warrants further investigation. There is very-low-quality evidence to support recommending FMT in IBS.
Bioinformatics and Machine Learning Methods to Identify FN1 as a Novel Biomarker of Aortic Valve Calcification
Authors: Xiong Tao , Han Shen , Pu Lei , Zhang Tian-Chen , Zhan Xu , Fu Tao , Dai Ying-Hai , Li Ya-Xiong
Published: February 2022

Aim

The purpose of this study was to identify potential diagnostic markers for aortic valve calcification (AVC) and to investigate the function of immune cell infiltration in this disease.

Methods

The AVC data sets were obtained from the Gene Expression Omnibus. The identification of differentially expressed genes (DEGs) and the performance of functional correlation analysis were carried out using the R software. To explore hub genes related to AVC, a protein–protein interaction network was created. Diagnostic markers for AVC were then screened and verified using the least absolute shrinkage and selection operator, logistic regression, support vector machine-recursive feature elimination algorithms, and hub genes. The infiltration of immune cells into AVC tissues was evaluated using CIBERSORT, and the correlation between diagnostic markers and infiltrating immune cells was analyzed. Finally, the Connectivity Map database was used to forecast the candidate small molecule drugs that might be used as prospective medications to treat AVC.

Results

A total of 337 DEGs were screened. The DEGs that were discovered were mostly related with atherosclerosis and arteriosclerotic cardiovascular disease, according to the analyses. Gene sets involved in the chemokine signaling pathway and cytokine–cytokine receptor interaction were differently active in AVC compared with control. As the diagnostic marker for AVC, fibronectin 1 (FN1) (area the curve = 0.958) was discovered. Immune cell infiltration analysis revealed that the AVC process may be mediated by naïve B cells, memory B cells, plasma cells, activated natural killer cells, monocytes, and macrophages M0. Additionally, FN1 expression was associated with memory B cells, M0 macrophages, activated mast cells, resting mast cells, monocytes, and activated natural killer cells. AVC may be reversed with the use of yohimbic acid, the most promising small molecule discovered so far.

Conclusion

FN1 can be used as a diagnostic marker for AVC. It has been shown that immune cell infiltration is important in the onset and progression of AVC, which may benefit in the improvement of AVC diagnosis and treatment.
Current Studies of Mitochondrial Quality Control in the Preeclampsia
Authors: Peng Xiaoqing , Hou Ruirui , Yang Yuanyuan , Luo Zhigang , Cao Yunxia
Published: February 2022

Mitochondria are cellular energy powerhouses that play important roles in regulating cellular processes. Mitochondrial quality control (mQC), including mitochondrial biogenesis, mitophagy, mitochondrial fusion and fission, maintains physiological demand and adapts to changed conditions. mQC has been widely investigated in neurodegeneration, cardiovascular disease and cancer because of the high demand for ATP in these diseases. Although placental implantation and fetal growth similarly require a large amount of energy, the investigation of mQC in placental-originated preeclampsia (PE) is limited. We elucidate mitochondrial morphology and function in different pregnancy stages, outline the role of mQC in cellular homeostasis and PE and summarize the current findings of mQC-related PE studies. This review also provides suggestions on the future investigation of mQC in PE, which will lead to the development of new prevention and therapy strategies for PE.
Differential Biological Effects of Dietary Lipids and Irradiation on the Aorta, Aortic Valve, and the Mitral Valve
Authors: Donis Nathalie , Jiang Zheshen , D'Emal Céline , Hulin Alexia , Debuisson Margaux , Dulgheru Raluca , Nguyen Mai-Linh , Postolache Adriana , Lallemand François , Coucke Philippe , Martinive Philippe , Herzog Marielle , Pamart Dorian , Terrell Jason , Pincemail Joel , Drion Pierre , Delvenne Philippe , Nchimi Alain , Lancellotti Patrizio , Oury Cécile
Published: February 2022

Aims

Dietary cholesterol and palmitic acid are risk factors for cardiovascular diseases (CVDs) affecting the arteries and the heart valves. The ionizing radiation that is frequently used as an anticancer treatment promotes CVD. The specific pathophysiology of these distinct disease manifestations is poorly understood. We, therefore, studied the biological effects of these dietary lipids and their cardiac irradiation on the arteries and the heart valves in the rabbit models of CVD.

Methods and Results

Cholesterol-enriched diet led to the thickening of the aortic wall and the aortic valve leaflets, immune cell infiltration in the aorta, mitral and aortic valves, as well as aortic valve calcification. Numerous cells expressing α-smooth muscle actin were detected in both the mitral and aortic valves. Lard-enriched diet induced massive aorta and aortic valve calcification, with no detectable immune cell infiltration. The addition of cardiac irradiation to the cholesterol diet yielded more calcification and more immune cell infiltrates in the atheroma and the aortic valve than cholesterol alone. RNA sequencing (RNAseq) analyses of aorta and heart valves revealed that a cholesterol-enriched diet mainly triggered inflammation-related biological processes in the aorta, aortic and mitral valves, which was further enhanced by cardiac irradiation. Lard-enriched diet rather affected calcification- and muscle-related processes in the aorta and aortic valve, respectively. Neutrophil count and systemic levels of platelet factor 4 and ent-8-iso-15(S)-PGF2α were identified as early biomarkers of cholesterol-induced tissue alterations, while cardiac irradiation resulted in elevated levels of circulating nucleosomes.

Conclusion

Dietary cholesterol, palmitic acid, and cardiac irradiation combined with a cholesterol-rich diet led to the development of distinct vascular and valvular lesions and changes in the circulating biomarkers. Hence, our study highlights unprecedented specificities related to common risk factors that underlie CVD.
Actionable Information in Flood Risk Communications and the Potential for New Web-Based Tools for Long-Term Planning for Individuals and Community
Authors: Mostafiz Rubayet Bin , Rohli Robert V. , Friedland Carol J. , Lee Yong-Cheol
Published: February 2022

Because of its ubiquitous nature and catastrophic impacts, flood information should be readily available and continually evaluated, to maximize utility for the public and professionals. Web-based tools can fill existing needs for actionable information to inform decisions regarding flood damage mitigation for new and existing structures. The goal of this research is to identify the current capabilities, gaps, and future demands of Web-based flood communication systems. To understand the current and potential niche of Web-based flood tools, a review of the literature concerning the effectiveness of mass media, grassroots-based “citizen science” efforts, and social media in communicating flood risk is conducted. Then, the strengths of 50 major, freely-available, Web-based flood information portals are reviewed. Results suggest that mass media often provide actionable information, especially for short-term benefit immediately before, during, and immediately after the flood for individuals and community leaders. Citizen science and grassroots efforts encourage planning strategies to prevent or mitigate flood. Social media is most beneficial in raising awareness of the flood hazard and communicating short- and long-term mitigation and adaptation strategies. However, while mass media, citizen science, and social media have revolutionized the way that people plan for, survive, and recover from floods, their utility is largely restricted to addressing short-term information needs at the meso-scale or broader and/or conveying information about singular events to scientists and/or other professional interests. Actionable information to inform long-term planning and mitigating flood, for both the public and community leaders, remains lacking. A particular need is for communication mechanisms that satisfy several criteria simultaneously: wide broadcast, appealing delivery method, and focus on enhancing decision-making for long-term needs rather than solely for short-term benefit. Particularly useful would be a new, webtool that provides sufficient functionality to enhance flood risk reduction decision making, considering both the costs and benefits of mitigation.
Deformation Prediction of Tunnel-Surrounding Rock Considering the Time Effect of the Viscosity Coefficient: A Case of an NATM-Excavated Tunnel
Authors: Hu Da , Liang Xiaoqiang , Li Yongsuo , Wu Youping , Jiang Lei
Published: February 2022

Considering engineering problems such as complicated stress and the difficulty in controlling large deformation while a tunnel passes through a soft rock stratum, a theoretical prediction model of convergence deformation of tunnel-surrounding rock is proposed. Based on the longitudinal displacement profile curve reflecting the “space effect” of the excavation surface, the Hoek formula with better applicability was introduced to analyze and theoretically deduct the “time–space effect comprehensively.” By taking the influence of the “time effect” coefficient into account, an improved Nishihara model was established to derive the analytical equation of the viscoelastic–viscoplastic convergence of surrounding rock. Taking the Dingxi Tunnel of Wujing Expressway in Hunan Province, the physical and mechanical parameters of surrounding rock in the tunnel were firstly determined then they were used to calculate and predict the vault down of three typical sections with the scoping equation of surrounding rock deformation. Based on the calculation results, the causes of the differences between the measured and theoretical values were analyzed; moreover, it is indicated that the minimum root-mean-square error is 1.68, the minimum average error is 1.3%, and the correlation coefficient is 0.99. The comparison shows that the theoretical prediction results agree well with the corresponding field test results. The improved Nishihara model can accurately predict the final deformation of the surrounding rock. Simultaneously, it is also proved that the relevant parameters and the hypothesis and correlation of the nonlinear viscosity coefficient equation are reasonable, with particular effectiveness and applicability in practical engineering. This study is significant for further studying the tunnel-surrounding rock’s stability and accumulating theoretical and practical experience to develop rheological theory.
Nanopore Structure and Mechanical Properties in Brittle Tectonically Deformed Coals Explored by Atomic Force Microscopy
Authors: Zhang Ningyuan , Yao Suping , Wang Yuhui
Published: February 2022

Tectonically deformed coals (TDCs) are of great importance to coalbed methane exploitation and coal mining safety. Compared to primary coals, reservoir properties of TDCs have been transformed greatly by tectonic stress. Here, the pore structure and mechanical properties of primary coal and brittle TDCs were obtained with atomic force microscopy and software. The results showed that tectonic stress generally promotes pore development and Young’s modulus of brittle TDCs. According to the variation in pore structure and Young’s modulus, two stages were identified: weak brittle deformation stage (primary coal–cataclastic coal–schistose coal–mortar coal) and strong brittle deformation stage (mortar coal–granulitic coal–flaky coal). The extent of tectonic impact varies greatly between these two stages. In weak brittle deformation stage, tectonic stress has little impact on coal pore structure. The mean pore number increases slowly, and the mean pore size decreases slowly. In this stage, half of the pore number increment is provided by macropores of 100–200 nm diameter. In strong brittle deformation stage, tectonic stress has a major impact on coal pore structure. The mean pore number increases quickly, and the mean pore size decreases quickly. Most of the pore number increment comes from mesopores of 10–50 nm diameter and macropores of 50–100 nm diameter. In addition, the Young’s modulus of primary coal and different brittle TDCs varies from 1.5 to 2.0 GPa. In weak brittle deformation stage, the Young’s modulus of different samples levels off. While in strong brittle deformation stage, the Young’s modulus increases gradually. Combined with former studies, it is inferred that tectonic stress can influence coal’s Young’s modulus by transforming its chemical structure.
A Gray Wolf Optimization-Based Improved Probabilistic Neural Network Algorithm for Surrounding Rock Squeezing Classification in Tunnel Engineering
Authors: Huang Xing , Yin Xin , Liu Bin , Ding Ziwei , Zhang Chaofan , Jing Boyu , Guo Xiaosheng
Published: February 2022

Surrounding rock squeezing deformation is a common and prominent hazard in tunnel engineering projects, which often induces the shield jamming disaster during the TBM tunneling process. Based on the 139 groups of historical squeezing deformation cases, this study developed a hybrid PCA-IWGO-PNN model for squeezing classification. According to the influencing factors and characteristics of squeezing deformation, the strength-stress ratio, tunnel burial depth, tunnel equivalent diameter, rock mass quality index, and support stiffness were selected to establish the prediction index system of squeezing level. Because the probabilistic neural network (PNN) requires that the input variables are independent, principal component analysis (PCA) was used to preprocess the original data to eliminate the correlation between prediction indexes and achieve dimensionality reduction. The spread coefficient was the critical hyper-parameter in the PNN, and the improved gray wolf optimization (IGWO) algorithm was used to realize its efficient automatic optimization. Then, the PNN model was applied to engineering practice. Only 1 of 20 test samples was misjudged, achieving the 95% prediction accuracy. Finally, the comparison analysis with the artificial neural network (ANN) model, support vector machine (SVM) model, and random forest (RF) model was conducted. Among them, the PNN model achieved the highest prediction accuracy, followed by the artificial neural network (85%), RF (85%), and SVM (80%). In addition, the PNN model had the fastest running speed, which only consumed 5.6350 s, while the running time of ANN, SVM, and RF was 8.8340, 6.2290, and 6.9260 s, respectively. The hybrid PCA-IWGO-PNN model developed in this research provides an effective method for surrounding rock squeezing classification, and it has superiorities in both prediction accuracy and running speed.
MgO-Coated Layered Cathode Oxide With Enhanced Stability for Sodium-Ion Batteries
Authors: Xue Ling , Bao Shuo , Yan Ling , Zhang Yi , Lu Jinlin , Yin Yansheng
Published: February 2022

Na0.67Ni0.33Mn0.67O2 is a prospective layered cathode material for sodium-ion batteries owing to its low cost, ease of synthesis, and high specific capacity. However, due to direct contact with electrolytes during the cycling process, the cyclic stability is not satisfied. To address this issue, magnesium oxide (MgO) surface modification was performed in this study to improve the material’s cycling properties. MgO layers of various thicknesses were successfully coated onto the cathode, and their electrochemical performances were thoroughly investigated. Among the as-prepared samples, the 2 wt% MgO-coated sample demonstrated the best rate capability and cycling stability. It had an initial reversible discharge capacity of 105 mAh g−1 in the voltage range from 2.0 to 4.5 V at 0.2 C with a high cycle retention of 81.5%. Electrochemical impedance spectroscopy (EIS) results showed that the 2 wt% MgO-coated electrode had the highest conductivity due to the smaller charge transfer resistance (Rct) value. All the test results show that the MgO modification improves the electrochemical properties of Na0.67Ni0.33Mn0.67O2 cathode material. This research could lead to the development of a promising strategy for improving the electrochemical performance of next-generation sodium-ion batteries.
RETRACTED: The Impact of Geopolitical and Pandemic Risks on Tourist Inflows: Evidence From Asia-Pacific Region
Authors: Wang Hong , Khan Yousaf Ali , Mouldi Abir , Abou El Khier Bothaina Samih , Guo Wei
Published: February 2022

The aim of this research is to investigate the impact of major risks such as geopolitical and pandemic risks on tourism industry in selected countries of Asia-Pacific. The results were obtained using a sample of 10 neighboring countries in the period of 1996 to 2018. Diagnostic tests confirmed the existence of spatial interaction between model variables for these countries. According to the results, the economic growth, internally and in neighboring countries, leads to an increase in the influx of tourists. Whereas increased domestic prices will reduce the influx of domestic tourists, rising prices in neighboring countries intensify tourists’ arrival of the country. Furthermore, results reveal that domestic geopolitical and pandemic risks lead to a decrease in tourist arrivals. In addition, whereas pandemic risks in neighboring countries do not have a significant effect on tourist arrivals, geopolitical risks in neighboring countries lead to more domestic tourists’ arrival.
Comparative Analysis of Trade Liberalization, CO2 Emissions, Energy Consumption and Economic Growth in Southeast Asian and Latin American Regions: A Structural Equation Modeling Approach
Authors: Zeeshan Muhammad , Han Jiabin , Rehman Alam , Ullah Irfan , Afridi Fakhr E. Alam , Fareed Zeeshan
Published: February 2022

This study compares the nexus among trade liberalization, CO2 emissions, energy consumption, and economic growth in Southeast Asian and Latin American countries. We apply the structural equation modeling approach for estimation analysis of the data from 1991 to 2018. The empirical findings of this study validate that trade has a positive and statistically significant effect on energy consumption, CO2 emissions, and gross domestic product (GDP) in Southeast Asian countries. Whereas in Latin American countries, trade shows a positive insignificant impact on energy consumption, but the coefficients for both CO2 emissions and GDP are positive and statistically significant. Energy consumption also exhibits a positive significant effect on CO2 emissions and a positive statistically insignificant effect on GDP in the Southeast Asian region. However, in Latin American countries, energy consumption predicts a positive and statistically significant impact on both CO2 emissions and GDP. Whereas, CO2 emissions indicate a positive significant effect on GDP in both regions. Therefore, each country’s government in both areas should formulate appropriate policies to promote green technologies in the production and exports, which could help economies to achieve a clean environment and sustainable long-term development.
Network Pharmacology and Molecular Docking Elucidate the Pharmacological Mechanism of the OSTEOWONDER Capsule for Treating Osteoporosis
Authors: Fan Jiashuang , Zhou Jianli , Qu Zhuan , Peng Hangya , Meng Shuhui , Peng Yaping , Liu Tengyan , Luo Qiu , Dai Lifen
Published: February 2022

Background: Osteoporosis (OP) is a serious and common bone metabolic disease with bone mass loss and bone microarchitectural deterioration. The OSTEOWONDER capsule is clinically used to treat OP. However, the potential regulatory mechanism of the OSTEOWONDER capsule in treatment of OP remains largely unknown.

Methods: The bioactive compounds of herbs and their targets were identified using the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP) database. The speculative targets of OP were screened out based on GeneCards, DisGeNET, and Online Mendelian Inheritance in Man (OMIM) databases. The gene modules and hub genes of OP were identified using a weighted gene co-expression network analysis (WGCNA). Then, an herb-compound-target network was constructed based on the above analyses. The biological function of targets was subsequently investigated, and a protein–protein interaction (PPI) network was constructed to identify hub targets of OP. Finally, molecular docking was performed to explore the interaction between compounds and targets.

Results: A total of 148 compounds of eight herbs and the corresponding 273 targets were identified based on the TCMSP database. A total of 4,929 targets of OP were obtained based on GeneCards, DisGeNET, and OMIM databases. In addition, six gene modules and 4,235 hub genes of OP were screened out based on WGCNA. Generally, an herb-compound-target network, including eight herbs, 84 compounds, and 58 targets, was constructed to investigate the therapeutic mechanism of the OSTEOWONDER capsule for OP. The biofunction analysis indicated 58 targets mainly associated with the bone metabolism, stimulation response, and immune response. EGFR, HIF1A, MAPK8, IL6, and PPARG were identified as the hub therapeutic targets in OP. Moreover, the interaction between EGFR, HIF1A, MAPK8, IL6, PPARG, and the corresponding compounds (quercetin and nobiletin) was analyzed using molecular docking.

Conclusion: Our finding discovered the possible therapeutic mechanisms of the OSTEOWONDER capsule and supplied the potential therapeutic targets for OP.
Differently Expressed Circular RNAs in Lacrimal Sacs From Patients With Chronic Dacryocystitis
Authors: Li Yue , Liu Xueru , Zhang Wenyue , Song Xuefei , Zhang Leilei , Xiao Caiwen
Published: February 2022

This study was designed to identify differently expressed circular RNAs (circRNAs) and investigate their potential roles in lacrimal sacs from patients with chronic dacryocystitis. The lacrimal sac samples of three chronic dacryocystitis patients and three control subjects were collected for RNA sequencing after ribosomal RNA was depleted. Differently expressed circRNAs and messenger RNAs (mRNAs) were used for co-expression analysis. CircRNA-microRNA (miRNA)-mRNA interaction network were also established by miRanda software. Meanwhile, pathway and functional enrichment analysis were conducted for the down- and up-regulated mRNAs in the circRNA-mRNA co-expression network. The expression levels of circRNAs and mRNAs in chronic dacryocystitis and control samples were validated by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). In all the 3,909 circRNAs predicted through RNA sequencing, 25 circRNAs (20 up-regulated and 5 down-regulated) expressed differently in chronic dacryocystitis samples. Besides, there identified 1,486 differentially expressed mRNAs. Of these differently expressed circRNAs and mRNAs, eight were validated by qRT-PCR, including MYH2, DSP, CD27, CCL5, FN1, has_circ_0004792, has_circ_0001062, and has_circ_0115476. Gene Ontology (GO) analysis indicated that the majority of altered mRNAs in this co-expression network were involved in immune system processes and meanwhile Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that these altered expressed mRNAs were also amplified in bacterial invasion of epithelial cells, both of which were thought to be involved in the pathogenesis of chronic dacryocystitis. In the circRNA-miRNA-mRNA interaction network, six circRNAs were found to be related to Th1 and Th2 cell differentiation, which was closely associated with the development of chronic dacryocystitis. This study identified statistically significant differences between circRNAs and mRNAs of lacrimal sac samples of chronic dacryocystitis patients and control individuals and provides novel insight into the regulatory mechanism of circRNAs, miRNAs, and mRNAs in the pathogenesis of chronic dacryocystitis.