bluuebunny/crossref_metadata_2025 huggingface dataset:
Black Youth Poetry of 2020 and Reimagined Literacies
Authors: Sciurba Katie
Published: October 2021

In response to anti-Black policing in 2020 that led to the deaths of George Floyd and Breonna Taylor, Black children and teens turned to poetry as a means to channel their self-described terror, rage, pain, horror, tiredness, and need for change. Reminiscent of the poetry of the Black Arts Movement and works published in The Black Panther newspaper, these poems, many of which call for a “revolution,” are reflective of young people’s critical engagements with the world and the word. With critical literacy as a framework, I engage in critical discourse analysis to determine how the young poets reimagine literacy as they protest anti-Black policing and racism. By focusing on young people’s own grassroots literacy initiatives, which call for the reimagination of blackness and whiteness, and demand truth, justice, and reimagined futures, I demonstrate how educators can reimagine literacy practices to center students’ criticalities and prioritize racial justice.
The Effects of Neurofeedback on Executive Functioning in Children With ADHD: A Meta-Analysis
Authors: Louthrenoo Orawan , Boonchooduang Nonglak , Likhitweerawong Narueporn , Charoenkwan Kittipat , Srisurapanont Manit
Published: October 2021

Objective:

Possible beneficial effects of neurofeedback in improving ADHD functional outcomes have been increasingly reported. This meta-analysis aimed to evaluate the relationship between neurofeedback and executive functioning in children with ADHD.

Methods:

PubMed, EMBASE, EBSCO, Web of Science, and Cochrane databases were searched to identify studies reporting the effects of neurofeedback on executive functioning, including response inhibition, sustained attention, and working memory, assessed by neuropsychological tests. Only randomized controlled studies of children aged 5 to 18 years were included using a random-effects model.

Results:

Ten studies were included. The effects of neurofeedback were not found on three domains of executive functions. A meta-regression analysis revealed a trend of numbers of neurofeedback sessions positively associated with response inhibition ( p = .06).

Conclusion:

Results did not show the benefits of neurofeedback on executive functions assessed by neuropsychological tests. Future studies should focus on standard neurofeedback protocols, the intensity of intervention, and neuropsychological outcomes.
NAD+, Senolytics, or Pyruvate for Healthy Aging?
Authors: Zhou Fang-Qiang
Published: October 2021

In last decades, healthy aging has become one of research hotspots in life science. It is well known that the nicotinamide adenine dinucleotide oxidized form (NAD+) level in cells decreases with aging and aging-related diseases. Several years ago, one of NAD+ precursors was first demonstrated with its new role in DNA damage repairing in mice, restoring old mice to their physical state at young ones. The finding encourages extensive studies in animal models and patients. NAD+ and its precursors have been popular products in nutrition markets. Alternatively, it was also evidenced that clearance of cellular senescence by senolytics preserved multiorgan (kidney and heart) function and extended healthy lifespan in mice. Subsequent studies confirmed findings in elderly patients subjected with idiopathic pulmonary fibrosis. The senolytic therapy is now focused on various diseases in animal and clinical studies. However, pyruvate, as both a NAD+ substitute and a new senolytic, may be advantageous, on the equimolar basis, over current products above in preventing and treating diseases and aging. Pyruvate-enriched fluids, particularly pyruvate oral rehydration salt, may be a novel intervention for diseases and aging besides critical care. Albeit the direct evidence that benefits healthy aging is still limited to date, pyruvate, as both NAD+ provider and senolytic agent, warrants intensive research to compare NAD+ or senolytics for healthy aging, specifically on the equimolar basis, in effective blood levels. This review briefly discussed the recognition of healthy aging by comparing NAD+ and Senolytics with sodium pyruvate from the clinical point of view.
Elevated sCD40L in Secondary Progressive Multiple Sclerosis in Comparison to Non-progressive Benign and Relapsing Remitting Multiple Sclerosis
Authors: Wu Qi , Wang Qin , Yang Jennifer , Martens Jacob WS , Mills Elizabeth A , Saad Aiya , Chilukuri Pavani , Dowling Catherine A , Mao-Draayer Yang
Published: October 2021

Background:

The long-term prognosis of relapsing-remitting multiple sclerosis (RRMS) is usually unfavorable as most patients transition to secondary progressive multiple sclerosis (SPMS) with accumulative disability. A rare form of non-progressive multiple sclerosis (MS) also exists, known as benign MS (BMS or NPMS), which lacks disease progression defined as Expanded Disability Status Scale (EDSS) ≤3 after 15 years of disease onset without treatment.

Purpose:

Our study aims to identify soluble plasma factors predicting disease progression in multiple sclerosis (MS).

Research Design and Study Sample:

We utilized Luminex multiplex to analyze plasma levels of 33 soluble factors, comparing 32 SPMS patients to age-, sex-, and disease duration-matched non-progressive BMS patients, as well as to RRMS patients and healthy controls.

Results:

Plasma levels of EGF, sCD40L, MCP1/CCL2, fractalkine/CX3CL1, IL-13, Eotaxin, TNFβ/LTα, and IL-12p40 were significantly different between the various types of MS. Plasma sCD40L was significantly elevated in SPMS compared to BMS and RRMS. The combination of MCP1/CCL2 and sCD40L discriminated between RRMS and SPMS. MCP1/CCL2 was found to be the most effective classifier between BMS and RRMS, while BMS was most effectively distinguished from SPMS by the combination of sCD40L and IFNγ levels.

Conclusions:

These differences may facilitate personalized precision medicine and aid in the discovery of new therapeutic targets for disease progression through the improvement of patient stratification.
‘Strikingly and stubbornly high’: Investigating the paradox of public confidence in the Irish police
Authors: Hamilton Claire , Black Lynsey
Published: October 2021

While levels of public confidence in the police have declined internationally, the Republic of Ireland appears to have bucked this trend with confidence levels that remain ‘strikingly and stubbornly high’ ( Mulcahy, 2016 : 275). This situation appears all the more puzzling given the wave of scandals to have hit the force in recent decades, ranging from police corruption in Donegal in the late 1990s to a more recent whistleblower scandal that has resulted in the resignation of a slew of Ministers and high-ranking officials. Such developments beg important questions as to the factors sustaining public confidence over this tumultuous period. Drawing on international and domestic data, this article aims to probe this ‘paradox’ of public confidence in the Irish police. It argues that, although confidence is high, there is more to the dynamics of confidence in the police in Ireland than this initial appraisal suggests. Indeed, it advances the Irish case as an illustration both of the dimensionality of the public confidence concept and the complexity of the pathways to trust in the police.
Transnationalism with Multi-Leveled Actors: A Systematic Review of Studies on Overseas Koreans’ Voting
Authors: Jang Sou Hyun
Published: October 2021

COVID-19 has affected several aspects of people’s lives, including overseas voting by Koreans overseas. Compared to the high national voter turnout for Korea’s 21st legislative elections (62.6%), the voter turnout among overseas Koreans was only 23.8%, the lowest since the inception of overseas voting in 2012. However, no study has systematically reviewed prior studies on overseas voting through the framework of political transnationalism with multilevel actors. By reviewing articles published between 2012 and 2020 that explore overseas Koreans’ voting in their home country’s election, this study aims to categorize studies at different levels and suggest policy implications and future directions for research that could contribute toward filling the gaps in the existing literature. The methods of overseas voting need to be diversified to adequately prepare for any exogenous shocks, such as COVID-19, during the upcoming election periods.
Did Juvenile Domestic Violence Offending Change During COVID-19?
Authors: Baglivio Michael T. , Wolff Kevin T. , Reid Joan A. , Jackson Sherry L. , Piquero Alex R.
Published: October 2021

The current study castssome of the first light into the initial impacts of the largest global health crisis in a generation on family and domestic violence, the long-term repercussions of which may take decades to unpack. Statewide trends in juvenile arrests for domestic violence (DV)-related offending are examined, taking into account school closures for in-person learning in March 2020 and the subsequent mandate for an in-person learning option in Florida in August 2020. Additionally, trends by sex, race/ethnicity, and severity of the offense are examined. Contrasting with growing studies demonstrating an increase in DV-related arrests among adults, we find a significant decrease upon school closures then subsequent increase when schools reopened with an in-person option. Results held across examined subgroups, yet the extent of increase following mandatory in-person learning availability was not as uniform, with Hispanic youth showing the smallest increase and Black youth the largest. Implications are discussed.
Gold nanotheranostics: future emblem of cancer nanomedicine
Authors: Navyatha Bankuru , Nara Seema
Published: October 2021

Cancer nanotheranostics aims at providing alternative approaches to traditional cancer diagnostics and therapies. In this context, plasmonic nanostructures especially gold nanostructures are intensely explored due to their tunable shape, size and surface plasmon resonance (SPR), better photothermal therapy (PTT) and photodynamic therapy (PDT) ability, effective contrast enhancing ability in Magnetic Resonance imaging (MRI) and Computed Tomography (CT) scan. Despite rapid breakthroughs in gold nanostructures based theranostics of cancer, the translation of gold nanostructures from bench side to human applications is still questionable. The major obstacles that have been facing by nanotheranostics are specific targeting, poor resolution and photoinstability during PTT etc. In this regard, various encouraging studies have been carried out recently to overcome few of these obstacles. Use of gold nanocomposites also overcomes the limitations of gold nanostructure probes and emerged as good nanotheranostic probe. Hence, the present article discusses the advances in gold nanostructures based cancer theranostics and mainly emphasizes on the importance of gold nanocomposites which have been designed to decipher the past questions and limitations of in vivo gold nanotheranostics.
Pharmacological Role of Ostericum koreanum: A Short Viewpoint
Authors: Hosseini Nasab Narges , Shah Fahad Hassan , Kim Song Ja
Published: October 2021

Ostericum koreanum Maxim., a perennial medicinal plant native to Asian countries, is traditionally exploited in Korean Oriental and Chinese Herbal Medicine. It has been used in the treatment of neuralgia, respiratory problems, and joint pain due to its rich content of phytochemicals. Therefore, the significant role of compounds present in O. koreanum should not be overlooked to explore and develop drugs against diseases. The purpose of this review is to provide a reference for researchers and to describe the phytochemical constituents and pharmacological activity of O. koreanum. In this mini review, we have collected the data from 1980 to 2020 regarding the phytochemicals present and pharmacological activities of this plant. Our findings indicated that this plant possesses a rich source of phytochemicals that have significant pharmacological activities, such as anti-microbial, anti-inflammatory, anti-pyretic, anti-influenza, anti-cancer, and neuroprotective. These phytochemicals have promising pharmacological activity which should be further explored for the treatment of various diseases.
Synthesis and Cytotoxic Activity of Novel Betulin Derivatives Containing Hydrazide-Hydrazone Moieties
Authors: Wu Jiale , Wang Jiafeng , Han Yinglong , Lin Yu , Wang Jing , Bu Ming
Published: October 2021

A series of novel betulin derivatives containing hydrazide-hydrazone moieties were synthesized. All compounds were evaluated for their cytotoxicity against four human carcinoma cell lines (HepG2, A549, MCF-7 and HCT-116) and a normal human gastric epithelial cell line (GES-1). Among them, compound 6i was the most potent against HepG2 and MCF-7 cell lines, with IC50 values of 9.27 and 8.87 μM, respectively. The results suggest that the incorporation of a hydrazide-hydrazone side chain at the C-28 position of betulin is beneficial for compounds to display significant cytotoxicity. Compound 6i may be used as a promising skeleton for antitumor agents with improved efficacy.
Posttraumatic Osteoarthritis Damage in Mice: From Histological and Micro–Computed Tomodensitometric Changes to Gait Disturbance
Authors: Fouasson-Chailloux Alban , Dauty Marc , Bodic Benoit , Masson Martial , Maugars Yves , Metayer Benoit , Veziers Joëlle , Lesoeur Julie , Rannou François , Guicheux Jérôme , Vinatier Claire
Published: October 2021

Objectives

Osteoarthritis is a painful joint disease responsible for walking impairment. Its quantitative assessment by gait analysis in mice may be a relevant and noninvasive strategy to assess the disease severity. In this study, we aimed to determine the severity of osteoarthritis at the tissular and gait levels in unilateral and bilateral posttraumatic murine osteoarthritis.

Methods

Twenty-four C57BL/6 male mice were randomly assigned to 3 groups ( n = 8/group): controls, unilateral surgery, and bilateral surgery. Posttraumatic osteoarthritis was induced unilaterally or bilaterally by destabilization of the medial meniscus. Gait analysis was performed weekly with the CatWalkTM XT system until the 16th week after surgery. After animal sacrifices, histological and micro–computed tomographic assessment was performed.

Results

Operated knees showed a significant increase in the histological score compared with controls ( P < 0.001). Calcified anterior medial meniscal bone volume was higher on the ipsilateral side after unilateral destabilization of the medial meniscus ( P < 0.001) and on both sides after bilateral intervention ( P < 0.01). One week after surgery, the mice mean speed decreased significantly in both operated groups ( P < 0.001 and P < 0.05). In the unilateral group, a significant increase in the contralateral hind print area appeared from week 4 to week 16.

Conclusions

While bilateral destabilization of the medial meniscus induced no detectable gait modification except 1 week after surgery, unilateral model was responsible for a gait disturbance on the contralateral side. Further studies are needed to better define the place of the CatWalkTM in the evaluation of mouse models of osteoarthritis.
Association Between Blunted Glomerular Hyperfiltration in Pregnancy and Severe Maternal Morbidity—A Research Letter
Authors: Harel Ziv , Park Alison L. , Ray Joel G.
Published: October 2021

Background:

Glomerular hyperfiltration is one physiological adaptation to pregnancy, marked by a decline in serum creatinine (SCr) concentration by 16 weeks’ gestation. It is not known whether blunted glomerular hyperfiltration leads to adverse maternal outcomes, including severe maternal morbidity (SMM).

Objective:

To evaluate the association between blunted glomerular hyperfiltration and subsequent SMM or death.

Design:

Population-based cohort study

Setting:

Ontario, Canada, from 2008 to 2019.

Participants:

Included were births among women who had ≥ 1 SCr measured as an outpatient within 10 weeks before conception (“preconception”), and again, at 110/7 to 206/7 weeks’ gestation (“in-pregnancy”). Excluded were women who died before birth, who had end-stage renal disease or kidney transplantation before conception, or whose pre-pregnancy SCr was 125 μmol/L.

Exposure:

Net glomerular hyperfiltration defined as the preconception minus the in-pregnancy SCr.

Measures:

The primary study outcome was SMM or death arising from 23 weeks’ gestation up to 42 days after the index birth.

Methods:

Adjusted relative risks (aRRs) were calculated using Modified Poisson regression per 1-SD net blunting of glomerular hyperfiltration adjusting for important covariates.

Results:

A total of 10,323 births met all inclusion criteria. The mean (SD) SCr was 61.7 (11.0) μmol/L preconception, 48.0 (9.2) μmol/L in-pregnancy, and the mean net difference 13.6 (8.2) μmol/L. Among these births, the adjusted RR of SMM or death from 23 weeks’ gestation up to 42 days post-partum was 1.16 (95% confidence interval 1.14-1.30) per 1-SD (8.2 μmol/L) net blunting of glomerular hyperfiltration.

Limitations:

As SCr assessment is not a routine part of pregnancy care, its measurement could have been for a specific health condition thereby imparting selection bias.

Conclusions:

Blunted glomerular hyperfiltration in pregnancy may identify some women at higher risk of SMM. Further prospective research is needed about the implications of glomerular hyperfiltration in early pregnancy.
Emotional thinking as the foundation of consciousness in artificial intelligence
Authors: Zhou Zhenhua
Published: October 2021

Current theories of artificial intelligence (AI) generally exclude human emotions. The idea at the core of such theories could be described as ‘cognition is computing’; that is, that human psychological and symbolic representations and the operations involved in structuring such representations in human thinking and intelligence can be converted by AI into a series of cognitive symbolic representations and calculations in a manner that simulates human intelligence. However, after decades of development, the cognitive computing doctrine has encountered many difficulties, both in theory and in practice; in particular, it is far from approaching real human intelligence. Real human intelligence runs through the whole process of the emotions. The core and motivation of rational thinking are derived from the emotions. Intelligence without emotion neither exists nor is meaningful. For example, the idea of ‘hot thinking’ proposed by Paul Thagard, a philosopher of cognitive science, discusses the mechanism of the emotions in human cognition and the thinking process. Through an analysis from the perspectives of cognitive neurology, cognitive psychology and social anthropology, this article notes that there may be a type of thinking that could be called ‘emotional thinking’. This type of thinking includes complex emotional factors during the cognitive processes. The term is used to refer to the capacity to process information and use emotions to integrate information in order to arrive at the right decisions and reactions. This type of thinking can be divided into two types according to the role of cognition: positive and negative emotional thinking. That division reflects opposite forces in the cognitive process. In the future, ‘emotional computing’ will cause an important acceleration in the development of AI consciousness. The foundation of AI consciousness is emotional computing based on the simulation of emotional thinking.
Effect of Foreign Direct Investment on Bilateral Trade: Experience From Asian Emerging Economies
Authors: Sohail Hafiz M. , Zatullah Mir , Li Zengfu
Published: October 2021

This study examines the effects of foreign direct investment (FDI) on bilateral trade between East and South Asian emerging economies, including their related trading partners. We cover the bilateral data on trade and FDI from June 2001 to June 2019. We estimate an augmented gravity model of trade to examine the study sample. This study is the first to use the Mundlak approaches an alternative for the fixed effect model to empirically estimate the relationship between FDI and trade among the countries in the region. Results show that free trade agreements (FTAs) and the corruption perception index (CPI) significantly and positively affect bilateral trade. However, the distance variable has become insignificant after introducing the FTA variable to the model. This finding indicates that FTAs marginalize the effect of distance on bilateral trade between the member countries. Thus, policymakers in developing countries should encourage and liberalize FDI from developing countries to enhance the bilateral trade volume.
The Relationship of Internal Communication Satisfaction With Employee Engagement and Employer Attractiveness: Testing the Joint Mediating Effect of the Social Exchange Quality Indicators
Authors: Tkalac Verčič Ana , Galić Zvonimir , Žnidar Krešimir
Published: October 2021

In order to improve internal communication within organizations, it is necessary to understand it better. This study explores the potential impact of internal communication on employee engagement and employer attractiveness by testing the mediating effects of social exchange quality indicators. A survey of 1,805 employees was used to test the relationships between internal communication satisfaction, employee engagement, and employer attractiveness and the potential mediating variables; reflecting the perception of the social relationship between an organization and its employees. The results showed that communication satisfaction correlated positively with the social exchange quality indicators (psychological contract fulfillment and perceived organizational support) and both outcomes—employee engagement and employer attractiveness. Additionally, the social exchange quality indicators partially mediated the relationship between internal communication satisfaction and both employee engagement and employer attractiveness. Our findings were consistent with the theoretical models in which internal communication satisfaction leads to higher employee engagement and employer attractiveness.
To Divide or Not to Divide? A Case Report Using Near-Infrared Spectroscopy to Assist With Decision Making on Timing of Division of Forehead Flaps
Authors: Yakaback Spencer , Webb Carmen , Temple-Oberle Claire
Published: October 2021

Paramedian forehead flaps (PMFFs) are commonly performed procedures for the reconstruction of complex nasal defects and require multiple stages predicated on when the interpolated pedicle is no longer required to provide perfusion to the neo-nose. As the presence of the pedicle is disfiguring, the flap is therefore divided ideally as soon as safely possible, but that determination is challenging. Recently, a novel device that uses near-infrared spectroscopy (NIRS) to measure tissue oxygen saturation has been developed and may provide an accurate and cost-effective tool to assess tissue perfusion. Here we present the use of this technology in the management of 2 patients who underwent successful oncologic nasal reconstructions using PMFFs. Using the device as a clinical adjunct, we measured tissue oxygen saturation values in the flaps before and after pedicle division, which assisted us in deciding when to safely divide the flaps, as well as with post-division management, particularly when a low tissue oxygen saturation reading ultimately necessitated the use of nitroglycerine past to improve flap perfusion in one of our patients. Interestingly, the values we recorded corresponded well to previously published cut-off values for adequate tissue perfusion, which have to date only been reported for free flaps. To our knowledge, this is the first description of the use of NIRS in the division of PMFFs, which we overall found to be a useful and reliable adjunct to a clinical examination in assessing flap neovascularization.
New conditional, scenario-based, and non-conditional cumulative absolute velocity models for subduction tectonic settings
Authors: Liu Chenying , Macedo Jorge
Published: October 2021

The PEER NGA-Sub ground-motion intensity measure database is used to develop new conditional ground-motion models (CGMMs), a set of scenario-based models, and non-conditional models to estimate the cumulative absolute velocity ([Formula: see text]) of ground motions from subduction zone earthquakes. In the CGMMs, the median estimate of [Formula: see text] is conditioned on the estimated peak ground acceleration ([Formula: see text]), the time-averaged shear-wave velocity in the top 30 m of the soil ([Formula: see text]), the earthquake magnitude ([Formula: see text]), and the spectral acceleration at the period of 1 s ([Formula: see text]). Multiple scenario-based [Formula: see text] models are developed by combining the CGMMs with pseudo-spectral acceleration ([Formula: see text]) ground-motion models (GMMs) for [Formula: see text] and [Formula: see text] to directly estimate [Formula: see text] given an earthquake scenario and site conditions. Scenario-based [Formula: see text] models are capable of capturing the complex ground-motion effects (e.g. soil non-linearity and regionalization effects) included in their underlying [Formula: see text]/[Formula: see text] GMMs. This approach also ensures the consistency of the [Formula: see text] estimates with a [Formula: see text] design spectrum. In addition, two non-conditional [Formula: see text] GMMs are developed using Bayesian hierarchical regressions. Finally, we present comparisons between the developed models. The comparisons show that if non-conditional GMMs are properly constrained, they are consistent with scenario-based GMMs. The [Formula: see text] GMMs developed in this study advance the performance-based earthquake engineering practice in areas affected by subduction zone earthquakes.
A novel mechanism of thrombocytopenia by PS exposure through TMEM16F in sphingomyelin synthase 1 deficiency
Authors: Fujii Yoritaka , Taniguchi Makoto , Nagaya Shingo , Ueda Yoshibumi , Hashizume Chieko , Watanabe Ken , Takeya Hiroyuki , Kosaka Takeo , Okazaki Toshiro
Published: October 2021

Sphingomyelin synthase 1 (SMS1) contributes to the generation of membrane sphingomyelin (SM) and affects SM-mediated physiological functions. Here, we describe the hematologic phenotypes, such as reduced circulating platelets and dysfunctional hemostasis, in SMS1-deficient (SMS1-KO) mice. SMS1-KO mice display pathologic manifestations related to idiopathic thrombocytopenia (ITP), including relatively high amounts of peripheral blood reticulated platelets, enhanced megakaryopoiesis in the bone marrow and spleen, and splenomegaly. Deficiency of SMS1, but not SMS2, prevented SM production and enhanced phosphatidylserine (PS) externalization on the plasma membranes of platelets and megakaryocytes. Consequently, SMS1-KO platelets were excessively cleared by macrophages in the spleen. Multimer formation in the plasma membrane of TMEM16F, a known calcium (Ca2+)-activated nonselective ion channel and Ca2+-dependent PS scramblase, was enhanced; the result was PS externalization to outer leaflets through increased Ca2+ influx in immortalized mouse embryonic fibroblasts established from SMS1-KO mice (SMS1-KO tMEFs), as seen with SMS1-KO platelets. Thus, SMS1 deficiency changed the TMEM16F distribution on the membrane microdomain, regulating Ca2+ influx-dependent PS exposure. SMS1-KO tMEFs in which TMEM16F was knocked out by using the CRISPR/Cas9 system lacked both the Ca2+ influx and excess PS exposure seen in SMS1-KO tMEFs. Therefore, SM depletion on platelet membrane microdomains due to SMS1 deficiency enhanced PS externalization via a Ca2+ influx through TMEM16F activation, leading to elevated platelet clearance and causing hemostasis dysfunction through thrombocytopenia. Our current findings show that the SM-rich microdomain generated by SMS1 is a potent regulator of thrombocytopenia through TMEM16F, suggesting that its dysfunction may be a novel additional mechanism of ITP.
A Markov random field model for network-based differential expression analysis of single-cell RNA-seq data
Authors: Li Hongyu , Zhu Biqing , Xu Zhichao , Adams Taylor , Kaminski Naftali , Zhao Hongyu
Published: October 2021

Background

Recent development of single cell sequencing technologies has made it possible to identify genes with different expression (DE) levels at the cell type level between different groups of samples. In this article, we propose to borrow information through known biological networks to increase statistical power to identify differentially expressed genes (DEGs).

Results

We develop MRFscRNAseq, which is based on a Markov random field (MRF) model to appropriately accommodate gene network information as well as dependencies among cell types to identify cell-type specific DEGs. We implement an Expectation-Maximization (EM) algorithm with mean field-like approximation to estimate model parameters and a Gibbs sampler to infer DE status. Simulation study shows that our method has better power to detect cell-type specific DEGs than conventional methods while appropriately controlling type I error rate. The usefulness of our method is demonstrated through its application to study the pathogenesis and biological processes of idiopathic pulmonary fibrosis (IPF) using a single-cell RNA-sequencing (scRNA-seq) data set, which contains 18,150 protein-coding genes across 38 cell types on lung tissues from 32 IPF patients and 28 normal controls.

Conclusions

The proposed MRF model is implemented in the R package MRFscRNAseq available on GitHub. By utilizing gene-gene and cell-cell networks, our method increases statistical power to detect differentially expressed genes from scRNA-seq data.
Alternative splicing during fruit development among fleshy fruits
Authors: Yan Xiaomin , Bai Dan , Song Hongtao , Lin Kui , Pang Erli
Published: October 2021

Background

Alternative splicing (AS) is an important mechanism of posttranscriptional modification and dynamically regulates multiple physiological processes in plants, including fruit ripening. However, little is known about alternative splicing during fruit development in fleshy fruits.

Results

We studied the alternative splicing at the immature and ripe stages during fruit development in cucumber, melon, papaya and peach. We found that 14.96–17.48% of multiexon genes exhibited alternative splicing. Intron retention was not always the most frequent event, indicating that the alternative splicing pattern during different developmental process differs. Alternative splicing was significantly more prevalent at the ripe stage than at the immature stage in cucumber and melon, while the opposite trend was shown in papaya and peach, implying that developmental stages adopt different alternative splicing strategies for their specific functions. Some genes involved in fruit ripening underwent stage-specific alternative splicing, indicating that alternative splicing regulates fruits ripening. Conserved alternative splicing events did not appear to be stage-specific. Clustering fruit developmental stages across the four species based on alternative splicing profiles resulted in species-specific clustering, suggesting that diversification of alternative splicing contributes to lineage-specific evolution in fleshy fruits.

Conclusions

We obtained high quality transcriptomes and alternative splicing events during fruit development across the four species. Dynamics and nonconserved alternative splicing were discovered. The candidate stage-specific AS genes involved in fruit ripening will provide valuable insight into the roles of alternative splicing during the developmental processes of fleshy fruits.
The current practice of aspiration prophylaxis in obstetric anesthesia: a survey among non-physician anesthetic providers working in hospitals in Ethiopia
Authors: Hunie Metages , Fenta Efrem , Kibret Simegnew , Teshome Diriba
Published: October 2021

Background

Pulmonary aspiration is one of the most important complications of obstetric anesthesia. Prevention of pulmonary aspiration is commonly performed by the application of different anesthetic maneuvers and administration of drugs. This study aimed to assess the non-physician anesthetic providers current practice of aspiration prophylaxis during anesthesia for cesarean section in Ethiopia.

Methods

This survey study was conducted from October 01 to November 05, 2020, on a total of 490 anesthetic providers working in hospitals in Ethiopia. A structured checklist was used to collect data from non-physician anesthetic providers.

Results

Four hundred and ninety (490) anesthetic providers participated in our study. The majority of the respondents (84%) were working in the public sector. Most of the cesarean delivery was done under regional anesthesia and more than half of anesthetic providers in Ethiopia administered aspiration prophylaxis routinely. Metoclopramide was the most frequently given as a prophylaxis for pulmonary aspiration.

Conclusions

More than half of the anesthetic providers administered aspiration prophylaxis routinely. Metoclopramide was the commonest administered aspiration prophylaxis for parturients who underwent cesarean delivery to prevent aspiration.
The effect of digoxin on renal function in patients with heart failure
Authors: Shah Parin , Pellicori Pierpaolo , Hanning Ian , Zhang Jufen , Clark Andrew L. , Bhandari Sunil
Published: October 2021

Introduction

Digoxin is used in patients with chronic heart failure (CHF) who remain symptomatic despite optimal medical treatment. Impaired renal function is commonly associated with CHF. We investigated the relation between digoxin use and change in renal function over time in patients with CHF.

Methods

One thousand two hundred forty-one patients with symptoms and signs of CHF (average age 72 years (64% male), and median NTproBNP 1426 ng/l (interquartile range 632–2897) were divided into four groups: never on digoxin (N = 394); digoxin throughout (N = 449); started digoxin at some point after baseline (N = 367); and stopped digoxin at some point after baseline (N = 31). The rate of change of estimated glomerular filtration rate (eGFR) was calculated using linear regression.

Results

Patients on digoxin throughout had a significantly greater rate of decline in eGFR per year than patients not on digoxin throughout (mean (± standard deviation); − 5 (14) ml/min/1.73m2per yearv − 2 (11) ml/min/1.73m2per year,P = 0.02). In those patients who started digoxin during follow up, there was no significant difference in the rate of decline in eGFR before and after starting digoxin. There was no correlation between baseline eGFR (or rate of decline in eGFR) and age, haemoglobin or NTproBNP. Compared to patients taking both angiotensin-converting-enzyme inhibitor (ACEi) or angiotensin receptor blockers (ARB) and beta-blocker (BB), patients who were not taking an ACEi/ARB or BB had a numerically faster rate of decline in eGFR, although this was not statistically significant.

Conclusion

The rate of decline in renal function is greater in patients with CHF who are taking digoxin.
Trimester-specific reference ranges for thyroid hormones of pregnant females at tertiary care hospitals in Lahore, Pakistan
Authors: Mumtaz Asim , Sadiq Fauzia , Zaki Saima , Batool Hijab , Ibrahim Muhammad , Khurram Muhammad , Awan Usman Ayub , Saeed Kiran , Afzal Muhammad Sohail
Published: October 2021

Background

The significance of investigation for diagnosing and managing thyroid dysfunction in pregnant females has been extensively documented in the medical literature. This study aimed to determine trimester-specific reference ranges for thyroid-stimulating hormones (TSH), free T3 (FT3), and free T4 (FT4) in apparently healthy pregnant women attending tertiary care hospitals in Lahore.

Methods

This cross-sectional study was conducted at two tertiary care Hospitals in Lahore, Pakistan. In this multi-centric study, 500 pregnant females were initially enrolled from September 2019 to December 2019 who fulfilled the inclusion criteria. For measurement of serum FT3, FT4, thyroid stimulating hormone (TSH), anti-thyroid peroxidase (anti-TPO), and thyroglobulin antibodies, 5 ml of the blood sample was drawn, under aseptic conditions, from each subject using Maglumi 800 chemiluminescence immunoassay (CLIA) system.

Results

Out of 500 subjects, 23 subjects with positive anti-TPO, 19 subjects with anti-TG antibodies, and 12 subjects due to less volume of serum yielded from whole blood (serum less than 3 ml) were excluded from the analysis. Ten samples were hemolyzed and not included in the analysis. A total of 436 samples were examined for analysis. Of the remaining 436 subjects, 133 (30.5%) were from 1st trimester, 153 (35.1%) from 2nd trimester, and 150 (34.4%) from 3rd trimester. As the data were non-normal, the 2.5th, 50th, and 97.5th percentiles were calculated to express each group's results. Trimester specific range of TSH 0.168-4.294, 0.258-4.584 and 0.341-4.625 mIU/mL, FT31.857-4.408, 1.958-4.621 and 2.025-4.821 pmol/L and FT4 8.815-18.006, 8.306-17.341 and 7.402-17.292 pmol/L.

Conclusion

In this study, we established a trimester-specific reference range for our local population's thyroid function test. The results of this study have complemented the results of previous studies.
Life in a time of COVID: a mixed method study of the changes in lifestyle, mental and psychosocial health during and after lockdown in Western Australians
Authors: Bhoyroo Ranila , Chivers Paola , Millar Lynne , Bulsara Caroline , Piggott Ben , Lambert Michelle , Codde Jim
Published: October 2021

Background

Since the beginning of the COVID-19 pandemic, the Western Australian government imposed multiple restrictions that impacted daily life activities and the social life. The aim of this study was to examine the effects of COVID-19 lockdown on the community’s physical, mental and psychosocial health.

Methods

Approximately 2 months after a three-month lockdown, a cross-sectional study was opened to Western Australian adults for an 8-week period (25th August – 21 October 2020). Participants competed a 25-min questionnaire adapted from the Western Australia Health and Wellbeing Surveillance system. Participants provided information on their socio-demographic status, lifestyle behaviours, mental health, and psychosocial health during and post-lockdown. Open-ended questions explored key issues in greater detail. Changes between the lockdown and post-lockdown period were assessed using Wilcoxon signed rank test and One-Sample Kolmogorov-Smirnov Normal tests as appropriate. Sex differences were examined using the Mann-Whitney U test. A content analysis approach examined responses to the open-ended questions with frequencies and variations in responses determined using Chi-Square tests.

Results

A total of 547 complete responses were obtained. Compared to post-lockdown period, lockdown was associated with a significantly lower levels of physical activity, poorer mental well-being and sense of control over one’s life, and a higher level of loneliness. Similarly, during lockdown, there was a significantly higher consumption of junk food, soft drinks and alcoholic drinks but no change in fruit and vegetable intake. Participants recalled health campaigns on hand washing and social distancing and there was a retrospective view that more timely and informative campaigns on physical activity, nutrition and mental well-being should have been available during lockdown.

Conclusions

While advice on infection control measures were appropriately provided, there is a need for concurrent health promotional information to help combat the changes in physical, mental and psychosocial well-being observed during quarantine to prevent negative health consequences in the community even if there are minimal effects of the pandemic itself.
It’s what you do, not the way you do it – online versus face-to-face small group teaching in first year medical school
Authors: Torda Adrienne , Shulruf Boaz
Published: October 2021

Background

Major disruptions imposed on medical education by the COVID-19 pandemic and the rapid shift to online teaching in medical programs, necessitated need for evaluation of this format. In this study we directly compared knowledge outcomes, social outcomes, and wellbeing of first year student small group teaching in either face to face (f2f) or online format.

Methods

At the end of the first course of our medical program, students were invited to participate in an online questionnaire with 10 quantitative items and 1 qualitative item. These were analysed using Factor Analysis Pattern Matrix and linear regression to group items and assess relatedness. Qualitative responses were thematized using Qualtrics software (Qualtrics, Provo, UT, USA). Summative assessment results were compared, both between current cohorts to historical cohorts.

Results

From a cohort of 298 students there was a 77% response rate. Overall, there were no differences in knowledge gains, either between groups or when compared to historical cohorts. Questionnaire items fell reliably into groups that related to either learning outcomes, social outcomes, or wellbeing. Independent T tests showed that format for teaching (online versus f2f) had an impact on social outcomes but no direct impact on learning outcomes. Linear regression revealed that the social outcomes have a direct impact on wellbeing and almost the double the impact on learning outcomes than mode of learning i.e.. F2f or online (β = .448 and β = .232 respectively).

Conclusion

In this study, we were able to show with statistical strength that social outcomes for students such as engaging with peers and facilitator, contributing to the group, and making friends have a direct impact on wellbeing and indirectly impact learning outcomes (such as motivation, satisfaction, integration of knowledge). In a rapidly changing educational landscape, in our opinion, it is vital that these aspects are a focus of design and delivery of medical education. The data from this study supports the notion that activity design and the expertise of the teacher in facilitating the small group activities, has greater impact than the mode of educational delivery itself on students’ learning processes.
Evaluation of the implementation process of the mobile health platform ‘WelTel’ in six sites in East Africa and Canada using the modified consolidated framework for implementation research (mCFIR)
Authors: El Joueidi Samia , Bardosh Kevin , Musoke Richard , Tilahun Binyam , Abo Moslim Maryam , Gourlay Katie , MacMullin Alissa , Cook Victoria J. , Murray Melanie , Mbaraga Gilbert , Nsanzimana Sabin , Lester Richard
Published: October 2021

Background

Health systems globally are investing in integrating secure messaging platforms for virtual care in clinical practice. Implementation science is essential for adoption, scale-up, spread and maintenance of complex evidence-based solutions in clinics with evolving priorities. In response, the mobile Health (mHealth) Research Group modified the existing consolidated framework for implementation research (CFIR) to evaluate implementation of virtual health tools in clinical settings. WelTel® is an evidence-based digital health platform widely deployed in various geographical and health contexts. The objective is to identify the facilitators and barriers for implementing WelTel and to assess the application of the mCFIR tool in facilitating focus groups in different geographical and health settings.

Methods

Both qualitative and descriptive quantitative approaches were employed. Six mCFIR sessions were held in three countries with 51 key stakeholders. The mCFIR tool consists of 5 Domains and 25 constructs and was distributed through Qualtrics Experience Management (XM). “Performance” and “Importance” scores were valued on a scale of 0 to 10 (Mean ± SD). Descriptive analysis was conducted using R computing software. NVivo 12 Pro software was used to analyze mCFIR responses and to generate themes from the participants’ input.

Results

We observed a parallel trend in the scores of Importance and Performance. Of the five Domains, Domain 4 (End-user Characteristics) and Domain 3 (Inner Settings) scored highest in Importance (8.9 ± 0.5 and 8.6 ± 0.6, respectively) and Performance (7.6 ± 0.7 and 7.2 ± 1.3, respectively) for all sites. Domain 2 (Outer Setting) scored the lowest in both Importance and Performance for all sites (7.6 ± 0.4 and 5.6 ± 1.8). The thematic analysis produced the following themes: for areas of strengths, the themes brought up were timely diagnosis and response, cost-effectiveness, and user-friendliness. As for areas for improvement, the themes discussed were training, phone accessibility, stakeholder engagement, and literacy.

Conclusion

The mCFIR tool allowed for a comprehensive understanding of the barriers and facilitators to the implementation, reach, and scale-up of digital health tools. Amongst several important findings, we observed the value of bringing the perspectives of both end users (HCPs and patients) to the table across Domains.

Trial Registration: NCT02603536 – November 11, 2015: WelTelOAKTREE: Text Messaging to Support Patients With HIV/AIDS in British Columbia (WelTelOAKTREE). NCT01549457 – March 9, 2012: TB mHealth Study—Use of Cell Phones to Improve Compliance in Patients on LTBI Treatment.
An ADAMTS13 mutation that causes hereditary thrombotic thrombocytopenic purpura: a case report and literature review
Authors: Li Pengzhu , Jiang Jie , Xi Qiong , Yang Zuocheng
Published: October 2021

Background

Mutations in the ADAMTS13 gene can lead to an ADAMTS13 enzyme deficiency, which is related to Upshaw–Schulman syndrome (USS). USS is a common type of thrombotic thrombocytopenic purpura (TTP). Here we present a very rare case of TTP caused by 2 mutations in the ADAMTS13 gene. Besides, we reviewed and summarized previous pathogenic ADAMTS13 gene mutations associated with the TTP.

Case presentation

A 10-year-old female was admitted to the Third Xiangya Hospital of Central South University after experiencing discontinuous thrombocytopenia for 8 years, abnormal renal function for more than 2 years, cough for more than 10 days, and weakness of the left limb for 3 days. Gene sequencing shows the patient’s ADAMTS13 gene contains compound heterozygous nucleotide variations: c.1335delC (p. Phe445LeufsTer52) is a frameshift variation inherited from her father and c.2130C > G (p. Cys710Trp) is a missense variation inherited from her mother. The final diagnosis was USS.

Conclusions

Our study reports a very rare genetic TTP case caused by two compound heterozygous variants in the ADAMTS13 gene. The effect of these two mutations on the secretion of ADAMTS13 requires further in vitro experiments to confirm.