bluuebunny/crossref_metadata_2025 huggingface dataset:
Website Premia for Extensive Margins of International Firm Activities: Evidence for SMEs from 34 Countries
Authors: Wagner Joachim
Published: October 2022

This paper uses firm-level data from the Flash Eurobarometer 421 survey conducted in June 2015 in 34 European countries to investigate the link between having a website and international firm activities in small- and medium-sized enterprises (SMEs). We find that firms that are present on the web more often export, import, engage in research and development cooperation with international partners, work as subcontractors for firms from other countries, use firms in other countries as subcontractors, and perform foreign direct investments—both inside and outside the European Union. The estimated website premia are statistically highly significant after controlling for firm size, country, and sector of economic activity. Furthermore, the size of these premia can be considered to be large. Internationally active firms tend to have a website.
Νarrow Row Spacing and Cover Crops to Suppress Weeds and Improve Sulla (Hedysarum coronarium L.) Biomass Production
Authors: Gazoulis Ioannis , Kanatas Panagiotis , Antonopoulos Nikolaos , Tataridas Alexandros , Travlos Ilias
Published: October 2022

Sulla (Hedysarum coronarium L.) is a new candidate crop for biofuel production. A field trial was conducted in 2018–2020 in Pyrgos, Greece, and repeated in 2019–2021 to evaluate different row spacings and cover crops for weed management in sulla in a two-factor randomized complete block design (RCBD) with four replications. Four row spacings, namely 76-cm, 51-cm, 38-cm, and 19-cm, were assigned to the main plots. Three cover crops, namely farro wheat (Triticum turgidum subsp. dicoccum (Schrank ex Schübler) Thell.), common vetch (Vicia sativa L.), white mustard (Sinapis alba L.), and an untreated control, were assigned to the subplots. In the first year of sulla growth, weed biomass, sulla stem, and total dry matter yield (DMY) were affected by growing cycles (p-value ≤ 0.05). The 19- and 38-cm row spacings resulted in the lowest weed biomass and the highest stem and total sulla DMY in the first year of sulla growth. White mustard was the most weed-suppressive cover crop in both years and growing cycles followed by farro heat. The highest stem DMY was observed in subplots with white mustard in both years. The combination of 38 cm row spacing and white mustard as a cover crop resulted in the highest cumulative two-year sulla DMY (18.9 t ha−1). Further case studies are needed to evaluate more cultural practices for weed management in sulla and other major biomass crops under different soil and climatic conditions.
Effect of Water Injection on Turbine Inlet under Different Flight Conditions
Authors: Luo Jiamao , Huang Shengfang , Yang Shunhua , Zhang Wanzhou , Mu Zhongqiang
Published: October 2022

Numerical simulations were conducted to research the pre-cooling effects of water injection on the turbine inlet of a turbine-based combined cycle (TBCC) engine under different flight conditions. Then, the performance of the water injection pre-compressor cooling (WIPCC) engine was calculated by mathematical modelling with different water to air ratios (WAR). It was the first time that the mass injection field of the turbine inlet of a TBCC engine was simulated, and it was also the first time that the performance of a subcomponent turbine engine of a TBCC was assessed. The calculation results showed the relationship of the inlet temperature with respect to WAR, inlet length and flight Mach number. The strategy for inlet length and water mass flow was proposed in order to meet the requirements of pre-cooling. When the length of the turbine inlet was 10 times the diameter of the inlet exit, the air could be cooled by 167.5 K with WAR = 0.09. The highest evaporation ratio reached at 93%. Finally, the calculation results revealed the performance of the water-pre-cooled turbine engine, of which the flight envelope was expanded to Ma3.0 from Ma2.3 by pre-cooling. The engine thrust as well as the specific impulse were significantly improved. The thrust reached at 0.9 times the characteristic thrust meeting the TBCC mode transition requirement of thrust and working speed spectrum.
Adaptive Resilient Control of AC Microgrids under Unbounded Actuator Attacks
Authors: Zuo Shan , Zhang Yi , Wang Yichao
Published: October 2022

Existing secondary control methods using fault-tolerant and/or H∞ control techniques for multi-inverter microgrids generally assume bounded faults and/or disturbances. Herein, we study unknown unbounded attacks on the input channels of both frequency and voltage control loops of inverters that could deteriorate the cooperative performance and affect the microgrid stability. We propose a fully distributed attack-resilient control framework using adaptive control techniques that, using stability analysis with Lyapunov techniques, are shown to preserve the uniformly ultimately bounded consensus for frequency regulation and voltage containment. Moreover, the ultimate bound can be set by adjusting the tuning parameters. The proposed result is validated for a modified IEEE 34-bus test feeder benchmark system augmented with four inverters.
Impacts of Climatic Variability on Agricultural Total Factor Productivity Growth in the Southern United States
Authors: Joshi Kartik , Lachaud Michée A. , Solís Daniel , Alvarez Sergio
Published: October 2022

This study investigates the impact of climatic variability on both agricultural production and total factor productivity (TFP) in the Southern United States (US). It also aims at identifying the drivers of productivity in this region. The analysis is tailored to inform decision makers about effective policy options to increase and sustain productivity in this important agricultural region. We use data from the US Department of Agriculture, National Climatic Data Center, and US Geological Survey to estimate alternative stochastic production frontier models. The estimated parameters are then analyzed and used to compute and decompose TFP into several measures of efficiency. The results show that agricultural production in the Southern US is more responsive to labor and has been increasing at a 1.13 percent rate annually. The findings also suggest that while precipitation, on average, has a positive and significant impact on productivity, intra-annual variation in both temperature and precipitation, which can be considered as anomalies, has a negative and significant impact on production. The impact of climatic effects on productivity across states is mixed and technological progress has been the main driver of TFP growth. Findings indicate that climatic variability is having a negative impact on agricultural productivity in the Southern US, similar in magnitude to the positive impact of irrigation.
Dynamic Change Characteristics of Litter and Nutrient Return in Subtropical Evergreen Broad-Leaved Forest in Different Extreme Weather Disturbance Years in Ailao Mountain, Yunnan Province
Authors: Liu Xingyue , Wang Ziyuan , Liu Xi , Lu Zhiyun , Li Dawen , Gong Hede
Published: October 2022

By studying the dynamic change characteristics of litter production, composition, nutrient content, and return amount of different components in different extreme weather interference years of Ailao Mountain evergreen broad-leaved forest, the paper provides theoretical support for the post-disaster nutrient cycle, ecological recovery, and sustainable development of the subtropical mid-mountain humid evergreen broad-leaved forest. Square litter collectors were randomly set up to collect litter. After drying to a constant mass, we calculated the seasonal and annual litter volume and the contents of organic carbon (C), total nitrogen (N), total phosphorus (P), total potassium (k), total sulfur (S), total calcium (Ca), and total magnesium (Mg). Finally, the nutrient return amount is comprehensively calculated according to the litter amount and element content. We tracked dynamic changes in litter quantity, nutrient composition, and nutrient components across different years. The results showed that the amount of litter from 2005 to 2015 was 7704–8818 kg·hm−2, and the order of magnitude was: 2005 (normal year) > 2015 (extreme snow and ice weather interference) > 2010 (extreme drought weather interference); the composition mainly included branches, leaves, fruit (flowers), and other components (bark, moss, lichen, etc.), of which the proportion of leaves was the largest, accounting for 41.70%–61.52%; The monthly changes and total amounts in different years exhibited single or double peak changes, and the monthly litter components in different years showed significant seasonality. In this study, the nutrient content of litter was higher than that of litter branches each year. The total amount of litter and the nutrient concentration of each component are C, Ca, N, K, Mg, S, and P, from large to small. The order of nutrient return in different years was the same as that of litter, and the returns of nutrients in litter leaves were greater than that of litter branches. The ratio of nutrient returns of litter and litter branches from 2005 to 2010 was 2.03, 1.23, and 3.69, respectively. The research shows that the litter decreased correspondingly under the extreme weather disturbance, and the impact of the extreme dry weather disturbance was greater than that of the extreme ice and snow weather disturbance. However, the evergreen broad-leaved forest in the study area recovers well after being disturbed. The annual litter amount and nutrient return amount is similar to that of evergreen broad-leaved forests in the same latitude and normal years in other subtropical regions. The decomposition rate and seasonal dynamics of litter nutrients are not greatly affected by extreme weather.
Research Opportunity on Fractional Cover of Forest: A Bibliometric Review
Authors: Mohd Ghazali Norzalyta , Said Mohd Nizam Mohd , Wan Mohd Jaafar Wan Shafrina , Muhmad Kamarulzaman Aisyah Marliza , Saad Siti Nor Maizah
Published: October 2022

Forests are threatened globally by deforestation. Forest restoration at the landscape scale can reduce these threats. Ground-based and remote sensing inventories are needed to assess restoration success. Fractional canopy cover estimated from forest algorithms can be used to monitor forest loss, growth, and health via remote sensing. Various studies on the fractional cover of forest have been published. However, none has yet conducted a bibliometric analysis. Bibliometrics provide a detailed examination of a topic, pointing academics to new research possibilities. To the best of the authors’ knowledge, this is the first bibliometric study screening publications to assess the incidence of studies of the fractional cover of forests in Web of Science (WoS) and Scopus databases. This research analyses WoS and Scopus publications on the fractional cover of forest dating from 1984 to 2021. The current study uses the Bibliometrix R-package for citation metrics and analysis. The first paper on the fractional cover of forest was published in 1984 and annual publication numbers have risen since 2002. USA and China were the most active countries in the study of fractional cover of forests. A total of 955 documents from 69 countries with multiple languages were retrieved. Vegetation, forestry, and remote sensing were the most discussed topics. Findings suggest more studies on the fractional cover of forests algorithms should be conducted in tropical forest from developing countries.
Effect of Sorbic Acid, Ethanol, Molasses, Previously Fermented Juice and Combined Additives on Ensiling Characteristics and Nutritive Value of Napiergrass (Pennisetum purpureum) Silage
Authors: Zhang Lei , Li Xinbao , Wang Siran , Zhao Jie , Dong Zhihao , Zhao Qiang , Xu Yang , Pan Xiaoqing , Shao Tao
Published: October 2022

The effects of sorbic acid, ethanol, previously fermented juice and their combined additives on fermentation quality and residual water-soluble carbohydrates (WSC) of napiergrass (Pennisetum purpureum) were herein investigated. There were two experiments in total. The treatments of experiment 1 were as follows: control (no addition); sorbic acid at 0.1%, S; molasses at 1.0%, M; previously fermented juice at 5 mL/kg, P; SP; SM; SPM. The treatments of experiment 2 were as follows: control (no addition); ethanol at 1.5%, E; molasses at 1.0%, M; previously fermented juice at 5 mL/kg, P; EM; EP; EPM. The laboratory silos (10 L) were kept at room temperature (~25 °C), and opened after 120 days of ensiling and the chemical compositions of silage were analyzed. Sorbic acid addition significantly (p < 0.05) decreased the silage pH and increased the residual WSC and lactic acid (LA) contents as compared with control. Ethanol addition decreased pH, acetic acid (AA) and volatile fatty acids (VFAs) contents, and increased the ratio of LA/AA, LA and residual WSC contents. Molasses addition increased ratios of LA/AA and enhanced WSC content used by lactic acid bacteria (LAB). The PFJ addition increased AA and ammonia nitrogen/total nitrogen (AN/TN) and decreased the LA contents. Comparing the fermentation quality among all silages in the present study, the combined additives SM and EM performed best in improving the silage quality of napiergrass.
Acceptability Analysis of 3D-Printed Food in the Area of the Czech Republic Based on Survey
Authors: Tesikova Karolina , Jurkova Lucie , Dordevic Simona , Buchtova Hana , Tremlova Bohuslava , Dordevic Dani
Published: October 2022

The aim of the research was to observe consumer perceptions of 3D food printing and to highlight possible applications of this production. The questionnaire survey took place in the Czech Republic and was attended by 1156 respondents. The questionnaire was divided into six sections: (1) Socio-Demographic Data; (2) 3D Common Printing Awareness; (3) 3D Food Printing Awareness; (4) 3D Food Printing, Worries and Understanding; (5) Application; (6) Investments. Although awareness of 3D food printing is increasing, a very small fraction of respondents had encountered printed food in person (1.5%; n = 17). Respondents expressed concerns about the health benefits and the reduced prices of novel foods, and they perceived printed foods as ultra-processed foods (56.0%; n = 647). Concerns have also been raised about job losses due to the introduction of new technology. On the contrary, they perceived that quality raw materials would be used to prepare printed foods (52.4%; n = 606). Most respondents believed that printed foods would be visually appealing and would find application in several food industry sectors. Most respondents believed that 3D food printing is the future of the food sector (83.8%; n = 969). The gained results can be helpful for 3D food printer producers, as well as for future experiments dealing with 3D food printing issues.
Genome-Wide Identification and Characterization of the CCT Gene Family in Foxtail Millet (Setaria italica) Response to Diurnal Rhythm and Abiotic Stress
Authors: Li Yuntong , Yu Shumin , Zhang Qiyuan , Wang Ziwei , Liu Meiling , Zhang Ao , Dong Xiaomei , Fan Jinjuan , Zhu Yanshu , Ruan Yanye , Li Cong
Published: October 2022

The CCT gene family plays important roles in diurnal rhythm and abiotic stress response, affecting crop growth and development, and thus yield. However, little information is available on the CCT family in foxtail millet (Setaria italica). In the present study, we identified 37 putative SiCCT genes from the foxtail millet genome. A phylogenetic tree was constructed from the predicted full-length SiCCT amino acid sequences, together with CCT proteins from rice and Arabidopsis as representatives of monocotyledonous and dicotyledonous plants, respectively. Based on the conserved structure and phylogenetic relationships, 13, 5, and 19 SiCCT proteins were classified in the COL, PRR, and CMF subfamilies, respectively. The gene structure and protein conserved motifs analysis exhibited highly similar compositions within the same subfamily. Whole-genome duplication analysis indicated that segmental duplication events played an important role in the expansion of the CCT gene family in foxtail millet. Analysis of transcriptome data showed that 16 SiCCT genes had significant diurnal rhythm oscillations. Under abiotic stress and exogenous hormonal treatment, the expression of many CMF subfamily genes was significantly changed. Especially after drought treatment, the expression of CMF subfamily genes except SiCCT32 was significantly up-regulated. This work provides valuable information for further study of the molecular mechanism of diurnal rhythm regulation, abiotic stress responses, and the identification of candidate genes for foxtail millet molecular breeding.
Is Smoking Associated with Carpal Tunnel Syndrome? A Meta-Analysis
Authors: Lampainen Kaisa , Hulkkonen Sina , Ryhänen Jorma , Curti Stefania , Shiri Rahman
Published: October 2022

To date, the role of smoking in carpal tunnel syndrome (CTS) is unclear. The aim of this systematic review and meta-analysis was to assess the association between smoking and CTS. The literature searches were conducted in PubMed, Embase, and Scopus, from inception until October 2021. Three reviewers screened the titles, abstracts, and full-text articles and evaluated the methodological quality of the included studies. A random-effects meta-analysis was used, and heterogeneity across studies was examined using I2 statistic. A total of 31 (13 cross-sectional, 10 case-control, and 8 cohort) studies were qualified for meta-analysis. In a meta-analysis of cohort studies, the risk of CTS did not differ between current and never smokers (pooled hazard ratio (HR) 1.09, 95% CI 0.84–1.43), current and past/never smokers (HR 1.07, 95% CI 0.94–1.23), and past and never smokers (HR 1.12, 95% CI 0.83–1.49). Furthermore, a meta-analysis of case control studies found no difference in the risk of CTS between current and never smokers (pooled odds ratio (OR) 0.92, 95% CI 0.56–1.53), current and past/never smokers (OR 1.10, 95% CI 0.51–2.36), and past and never smokers (OR 0.91, 95% CI 0.59–1.39). However, a meta-analysis of cross-sectional studies showed the associations of ever (OR 1.36, 95% CI 1.08–1.72) and current smoking (OR 1.52, 95% CI 1.11–2.09) with CTS. However, the association between ever smoking and CTS disappeared after limiting the meta-analysis to higher quality studies or after adjusting for publication bias. The association between current smoking and CTS also attenuated after limiting the meta-analysis to studies that confirmed CTS by a nerve conduction study or studies with low attrition bias. This meta-analysis does not support an association between smoking and CTS. The association between smoking and CTS observed in cross-sectional studies could be due to biases and/or confounding factors.
The Geriatric Depression Scale Predicts Glycemic Control in Older Adult with Type 2 Diabetes Mellitus: A Longitudinal Study
Authors: Sirirak Thanitha , Sangsupawanich Pasuree , Wongpakaran Nahathai , Srisintorn Wisarut
Published: October 2022

The presence of comorbid depression and diabetes is associated with worse glycemic control, higher complication and greater mortality risk than expected by each condition alone. The association between various levels of severity of depressive symptoms and glycemic control over time among type 2 diabetic older patients was unclear. This study aimed to investigate a longitudinal association between depression and HbA1c among type 2 diabetic older patients. Type 2 diabetes patients aged 60 years and above with normal cognition were recruited from the outpatient department from 1 June 2020 to 1 July 2021. The Thai Geriatric Depression Scale (TGDS) and HbA1c were assessed at five time points (baseline and every 12 weeks) for 1 year. A linear mixed effect model was used. Of the 161 enrolled participants, 146 completed the study. At baseline, 14% were susceptible to depression or having depression (TGDS score 6 and above), and there was a significant correlation between HbA1c and depression (r = 0.26, p ≤ 0.01). The longitudinal analysis indicated that TGDS was a significant predictor of HbA1c in the next visit, and the relationship was J-shaped. A TGDS below 5 was associated with decreasing HbA1c in the next visit, but the association became positive at a TGDS score at 5 or higher. The presence of significant symptoms of depression was associated with glycemic control in the next 3-month interval OPD visit event, although major depressive disorder has not yet been established.
Child Marriage and Problems Accessing Healthcare in Adulthood: Evidence from India
Authors: Datta Biplab , Pandey Ajay , Tiwari Ashwini
Published: October 2022

The association between child marriage and the access to or utilization of maternal and antenatal healthcare has been widely studied. However, little is known about child brides’ access to healthcare for illnesses later in life. Using data on 496,283 married women aged 18 to 49 years from the India National Family and Health Survey 2015–2016, we developed an 11-point composite score (ranging from 0 to 10) outlining the extent of problems accessing healthcare, as follows: (i) no/little problem (score 0 to 2), (ii) some problems (score 3 to 6), and (iii) big problems (score 7 to 10). The differences between child brides and their peers married as adults were assessed by the relative risk ratios obtained from multinomial logistic regressions. The adjusted risk of having “some problems” and “big problems” accessing healthcare relative to “no/little problem” for child brides was found to be 1.22 (95% CI: 1.20–1.25) and 1.26 (95% CI: 1.22–1.29) times that of those married as adults, respectively. These findings highlight the disproportionate barriers to healthcare access faced by women married as children compared to women married as adults and the need for further research to inform policies regarding effective public health interventions to improve healthcare access.
Strawberry Production with Different Mulches and Wetted Areas
Authors: Pinto João Pedro , da Cunha Fernando França , da Silva Adão Alexandre , de Paula Laércio Boratto , Ribeiro Marcos Caldeira , Costa Neto José Ricardo Resende
Published: October 2022

The use of mulch contributes to the reduction of water consumption and weed infestation in strawberry cultivation. Recycled paper, being biodegradable, has great potential to replace plastics. Thus, the objective was to evaluate the water consumption and agronomic performance of strawberry subjected to different wetted areas and mulches. The wet areas tested were 40% (WA40) and 70% (WA70) imposed by a drip irrigation system. The different types of mulch were: white polyethylene (WHP), black polyethylene (BLP), recycled paper (REP) and no mulch (NM). BLP, REP and WHP mulches promoted the same weed control. The number of fruits per plant, fruit length, fruit yield, and water productivity did not differ for the factors wetted area and types of mulch. Higher fruit mass and diameter were found in the WA40 treatment, while the mulches favored only fruit mass. Thus, fruit yield showed no difference, and only water consumption differed between the wet areas and between the types of mulch. Strawberry water consumption was higher in WA70. In relation to fruit waste, it was found that the WHP and BLP mulches provided higher values than REP and NM. Thus, the recycled paper, combined with a wet area of 40%, is recommended as a mulch in strawberry production.
Factors Influencing O3 Concentration in Traffic and Urban Environments: A Case Study of Guangzhou City
Authors: Liu Tao , Sun Jia , Liu Baihua , Li Miao , Deng Yingbin , Jing Wenlong , Yang Ji
Published: October 2022

Ozone (O3) pollution is a serious issue in China, posing a significant threat to people’s health. Traffic emissions are the main pollutant source in urban areas. NOX and volatile organic compounds (VOCs) from traffic emissions are the main precursors of O3. Thus, it is crucial to investigate the relationship between traffic conditions and O3 pollution. This study focused on the potential relationship between O3 concentration and traffic conditions at a roadside and urban background in Guangzhou, one of the largest cities in China. The results demonstrated that no significant difference in the O3 concentration was observed between roadside and urban background environments. However, the O3 concentration was 2 to 3 times higher on sunny days (above 90 μg/m3) than on cloudy days due to meteorological conditions. The results confirmed that limiting traffic emissions may increase O3 concentrations in Guangzhou. Therefore, the focus should be on industrial, energy, and transportation emission mitigation and the influence of meteorological conditions to minimize O3 pollution. The results in this study provide some theoretical basis for mitigation emission policies in China.
Leaving Past Adversities Behind: Gratitude Intervention Compensates for the Undesirable Effects of Past Time Perspectives on Negative Affect
Authors: Burzynska-Tatjewska Bozena , Stolarski Maciej
Published: October 2022

Both gratitude and savoring the moment are considered to be well-established “well-being boosters” (WBBs). Each of them has a salient temporal reference: Gratitude is past-related, whereas savoring the moment refers to the present. The temporal match–mismatch model posits that time perspectives (TPs) moderate the effects of WBBs on well-being if they refer to the same temporal frame (e.g., gratitude and Past-Positive). Our study tested whether TPs moderate the effects of two positive interventions on positive affect (PA) and negative affect (NA). The participants (N = 174 individuals, 73% women) completed measures of TPs, PA, and NA, followed by a brief intervention of gratitude (N = 86) or savoring (N = 88). Subsequently, they completed the PA/NA measures again. Both interventions significantly decreased NA but did not foster PA. The magnitude of the shift in NA in the gratitude condition depended on the levels of past TPs: Individuals high in Past-Negative and low in Past-Positive displayed greater baseline NA than their counterparts; however, the difference was leveled after the gratitude intervention. No interaction effects between the present TPs and the savoring intervention were observed. The results suggest that practicing gratitude may diminish the undesirable consequences of negative views of the past.
Effect of Receiving Financial Support from Adult Children on Depression among Older Persons and the Mediating Role of Social Participation
Authors: Xia Wenran , van Wijngaarden Jeroen D. H. , Huijsman Robbert , Buljac-Samardžić Martina
Published: October 2022

Older persons are vulnerable to depression SFduring the ageing process. Financial resources and social participation are expected to have an impact on depressive symptoms. This study investigated the relationship between financial support from children and depression among Chinese older persons, as well as the mediating effect of social participation in this relationship. Data from 7163 participants aged 60 and above were extracted from wave 2015 and 2018 of the China Health and Retirement Longitudinal Survey (CHARLS). A multivariate regression analysis was performed on both cross-sectional data and two-wave longitudinal data to test our hypotheses. The results revealed that financial support from children was negatively associated with depressive symptoms in both the short-term and the long-term. In addition, this relationship was partially mediated by social participation in the short-term association and fully mediated by social participation in the long-term, where financial support was positively related to social participation, and social participation was negatively associated with depressive symptoms. This study offers an in-depth insight into the relationship between financial support from children and depression among Chinese older persons. Policies and initiatives to stimulate social participation should be promoted to improve older persons’ mental health.
Cocktail Effect of Endocrine Disrupting Chemicals: Application to Chlorpyrifos in Lavender Essential Oils
Authors: Fouyet Sophie , Olivier Elodie , Leproux Pascale , Boutefnouchet Sabrina , Dutot Mélody , Rat Patrice
Published: October 2022

Chlorpyrifos is a pesticide that is toxic to human health and has been banned for the past decade. Due to its persistent and bioaccumulative properties, chlorpyrifos is still present in soil. Pregnant women can be exposed to chlorpyrifos through drinking water and herbal products, such as essential oils (EOs), resulting in adverse effects to the mother and fetus. Our objective was to evaluate and compare the potential endocrine disrupting effects of chlorpyrifos “free” or in contaminated lavender EO. We studied the release of four hormones and the activation of the P2X7 cell death receptor in human placental JEG-Tox cells as key biomarkers of endocrine toxicity for pregnant women (hPlacentox assay). We observed that “free” chlorpyrifos disrupted placental hormones and activated the P2X7 receptor, whereas chlorpyrifos in lavender EO disrupted only the placental hormones. We confirm that chlorpyrifos can be classified as an endocrine disrupting chemical (EDC) for pregnant women and point out that its endocrine disrupting effect may not be apparent when present in lavender EOs. Our results reveal the existence of specific reverse cocktail effects that may have protective properties against EDCs.
The Impact of Biochar Used in Repairs to Historical Buildings on Public Health
Authors: Tokarski Daniel , Ickiewicz Irena , Żukiewicz-Sobczak Wioletta , Woliński Paweł
Published: October 2022

The subject matter of this manuscript concerns the analysis and identification of microorganisms that pose a threat to human health and, in particular, mold fungi occurring in historical buildings. Surfaces infected by fungal spores pose a threat to the structure and the health of both visitors to historical buildings and professionals working in them. Research was undertaken to fill in the defects in building partitions with a supplementary layer of biochar in order to eliminate, or partially reduce, the possibility of contamination with and development of harmful mold fungi. In the designed cement mixture, biochar was used as a filler, the task of which was to eliminate the causes that lead to the development of harmful mold fungi. Microbiological analyses of the surface of walls and air in selected buildings were carried out before and after the application of supplementary biochar layers. The inhibitory properties of the material used against the presence and growth of mold fungi were observed. The average number of microorganisms isolated on the tested partitions decreased by between 70 and 100%. As a consequence, the use of this material significantly influenced the air quality of the rooms, which is important for protecting the health of people at work, as well as those visiting historical buildings.
Prerequisite for COVID-19 Prediction: A Review on Factors Affecting the Infection Rate
Authors: Tang Shirley Gee Hoon , Hadi Muhamad Haziq Hasnul , Arsad Siti Rosilah , Ker Pin Jern , Ramanathan Santhi , Afandi Nayli Aliah Mohd , Afzal Madihah Mohd , Yaw Mei Wyin , Krishnan Prajindra Sankar , Chen Chai Phing , Tiong Sieh Kiong
Published: October 2022

Since the year 2020, coronavirus disease 2019 (COVID-19) has emerged as the dominant topic of discussion in the public and research domains. Intensive research has been carried out on several aspects of COVID-19, including vaccines, its transmission mechanism, detection of COVID-19 infection, and its infection rate and factors. The awareness of the public related to the COVID-19 infection factors enables the public to adhere to the standard operating procedures, while a full elucidation on the correlation of different factors to the infection rate facilitates effective measures to minimize the risk of COVID-19 infection by policy makers and enforcers. Hence, this paper aims to provide a comprehensive and analytical review of different factors affecting the COVID-19 infection rate. Furthermore, this review analyses factors which directly and indirectly affect the COVID-19 infection risk, such as physical distance, ventilation, face masks, meteorological factor, socioeconomic factor, vaccination, host factor, SARS-CoV-2 variants, and the availability of COVID-19 testing. Critical analysis was performed for the different factors by providing quantitative and qualitative studies. Lastly, the challenges of correlating each infection risk factor to the predicted risk of COVID-19 infection are discussed, and recommendations for further research works and interventions are outlined.
Nutraceutical-Based Nanoformulations for Breast and Ovarian Cancer Treatment
Authors: Serini Simona , Cassano Roberta , Curcio Federica , Trombino Sonia , Calviello Gabriella
Published: October 2022

Different strategies have been investigated for a more satisfactory treatment of advanced breast cancer, including the adjuvant use of omega-3 polyunsaturated fatty acids (PUFAs). These nutritional compounds have been shown to possess potent anti-inflammatory and antiangiogenic activities, the capacity to affect transduction pathways/receptors involved in cell growth and to reprogram tumor microenvironment. Omega-3 PUFA-containing nanoformulations designed for drug delivery in breast cancer were shown to potentiate the effects of enclosed drugs, enhance drug delivery to target sites, and minimize drug-induced side effects. We have critically analyzed here the results of the most recent studies investigating the effects of omega-3 PUFA-containing nanoformulations in breast cancer. The anti-neoplastic efficacy of omega-3 PUFAs has also been convincingly demonstrated by using preclinical in vivo models of ovarian cancer. The results obtained are critically analyzed here and seem to provide a sufficient rationale to move to still lacking interventional clinical trials, as well as to evaluate possible advantages of enclosing omega-3 PUFAs to drug-delivery nanosystems for ovarian cancer. Future perspectives in this area are also provided.
Interactions between C8orf37 and FAM161A, Two Ciliary Proteins Essential for Photoreceptor Survival
Authors: Liu Yu , Chen Jinjun , Sager Rachel , Sasaki Erika , Hu Huaiyu
Published: October 2022

Mutations in C8orf37 cause Bardet-Biedl syndrome (BBS), retinitis pigmentosa (RP), and cone–rod dystrophy (CRD), all manifest in photoreceptor degeneration. Little is known about which proteins C8orf37 interacts with to contribute to photoreceptor survival. To determine the proteins that potentially interact with C8orf37, we carried out a yeast two-hybrid (Y2H) screen using C8orf37 as a bait. FAM161A, a microtubule-binding protein localized at the photoreceptor cilium required for photoreceptor survival, was identified as one of the preys. Double immunofluorescence staining and proximity ligation assay (PLA) of marmoset retinal sections showed that C8orf37 was enriched and was co-localized with FAM161A at the ciliary base of photoreceptors. Epitope-tagged C8orf37 and FAM161A, expressed in HEK293 cells, were also found to be co-localized by double immunofluorescence staining and PLA. Furthermore, interaction domain mapping assays identified that the N-terminal region of C8orf37 and amino acid residues 341-517 within the PFAM UPF0564 domain of FAM161A were critical for C8orf37–FAM161A interaction. These data suggest that the two photoreceptor survival proteins, C8orf37 and FAM161A, interact with each other which may contribute to photoreceptor health.
Microsporum gypseum Isolated from Ailuropoda melanoleuca Provokes Inflammation and Triggers Th17 Adaptive Immunity Response
Authors: Ma Xiaoping , Liu Zhen , Yu Yan , Jiang Yaozhang , Wang Chengdong , Zuo Zhicai , Ling Shanshan , He Ming , Cao Sanjie , Wen Yiping , Zhao Qin , Wu Rui , Huang Xiaobo , Zhong Zhijun , Peng Guangneng , Gu Yu
Published: October 2022

Microsporum gypseum causes dermatomycoses in giant pandas (Ailuropoda melanoleuca). This study aimed to investigate the immune response of M. gypseum following deep infection. The degree of damage to the heart, liver, spleen, lungs, and kidneys was evaluated using tissue fungal load, organ index, and histopathological methods. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) detected the mRNA expression of receptors and cytokines in the lung, and immunofluorescence staining and flow cytometry, were used to assess immune cells in the lung. The results indicated that conidia mainly colonized the lungs and caused serious injury with M. gypseum infection. Furthermore, dectin-1, TLR-2, and TLR-4 played a role in recognizing M. gypseum cells. Numerous inflammatory cells, mainly macrophages, dendritic cells, polymorphonuclear neutrophils, and inflammatory cytokines (TGF-β, TNF-α, IL-1β, IL-6, IL-10, IL-12, and IL-23), were activated in the early stages of infection. With the high expression of IL-22, IL-17A, and IL-17F, the Th17 pathway exerted an adaptive immune response to M. gypseum infection. These results can potentially aid in the diagnosis and treatment of diseases caused by M. gypseum in giant pandas.
CircCSDE1 Regulates Proliferation and Differentiation of C2C12 Myoblasts by Sponging miR-21-3p
Authors: Sun Di , An Jiaqi , Cui Zixu , Li Jiao , You Ziwei , Lu Chang , Yang Yang , Gao Pengfei , Guo Xiaohong , Li Bugao , Cai Chunbo , Cao Guoqing
Published: October 2022

The growth and development of skeletal muscle is regulated by many factors, and recent studies have shown that circular RNAs (circRNAs) can participate in this process. The model of porcine skeletal muscle injury was constructed to search for circRNAs that can regulate the growth and development of skeletal muscle in pigs. Using whole-transcriptome sequencing and bioinformatics analysis, a novel circRNA (circCSDE1) was screened out, which is highly expressed in skeletal muscle. Functional studies in C2C12 cells demonstrated that circCSDE1 could promote proliferation and inhibit myoblast differentiation, while opposing changes were observed by circCSDE1 knockdown. A dual-luciferase reporter assay revealed that circCSDE1 directly targeted miR-21-3p to regulate the expression of the downstream target gene (Cyclin-dependent kinase 16, CDK16). Moreover, miR-21-3p could inhibit proliferation and promote myoblast differentiation in C2C12 cells, opposite with the effects of circCSDE1. Additionally, the rescue experiments offered further evidence that circCSDE1 and its target, miR-21-3p, work together to regulate myoblast proliferation and differentiation. This study provides a theoretical basis for further understanding the regulatory mechanisms of circRNAs.
Heme Oxygenase-1 as Therapeutic Target for Diabetic Foot Ulcers
Authors: Leal Ermelindo Carreira , Carvalho Eugenia
Published: October 2022

A diabetic foot ulcer (DFU) is one of the major complications of diabetes. Wound healing under diabetic conditions is often impaired. This is in part due to the excessive oxidative stress, prolonged inflammation, immune cell dysfunction, delayed re-epithelialization, and decreased angiogenesis present at the wound site. As a result of these multifactorial impaired healing pathways, it has been difficult to develop effective therapeutic strategies for DFU. Heme oxygenase-1 (HO-1) is the rate-limiting enzyme in heme degradation generating carbon monoxide (CO), biliverdin (BV) which is converted into bilirubin (BR), and iron. HO-1 is a potent antioxidant. It can act as an anti-inflammatory, proliferative, angiogenic and cytoprotective enzyme. Due to its biological functions, HO-1 plays a very important role in wound healing, in part mediated through the biologically active end products generated by its enzymatic activity, particularly CO, BV, and BR. Therapeutic strategies involving the activation of HO-1, or the topical application of its biologically active end products are important in diabetic wound healing. Therefore, HO-1 is an attractive therapeutic target for DFU treatment. This review will provide an overview and discussion of the importance of HO-1 as a therapeutic target for diabetic wound healing.
Usefulness of Urinary Biomarkers for Assessing Bladder Condition and Histopathology in Patients with Interstitial Cystitis/Bladder Pain Syndrome
Authors: Jiang Yuan-Hong , Jhang Jia-Fong , Hsu Yuan-Hsiang , Kuo Hann-Chorng
Published: October 2022

This study investigated the usefulness of urinary biomarkers for assessing bladder condition and histopathology in patients with interstitial cystitis/bladder pain syndrome (IC/BPS). We retrospectively enrolled 315 patients (267 women and 48 men) diagnosed with IC/BPS and 30 controls. Data on clinical and urodynamic characteristics (visual analog scale (VAS) score and bladder capacity) and cystoscopic hydrodistention findings (Hunner’s lesion, glomerulation grade, and maximal bladder capacity (MBC)) were recorded. Urine samples were utilized to assay inflammatory, neurogenic, and oxidative stress biomarkers, including interleukin (IL)-8, C-X-C motif chemokine ligand 10 (CXCL10), monocyte chemoattractant protein-1 (MCP-1), brain-derived neurotrophic factor (BDNF), eotaxin, IL-6, macrophage inflammatory protein 1 beta (MIP-1β), regulated on activation, normal T cell expressed and secreted (RANTES), tumor necrosis factor-alpha (TNF-α), prostaglandin E2 (PGE2), 8-hydroxy-2′-deoxyguanosine (8-OHdG), and 8-isoproatane, and total antioxidant capacity. Further, specific histopathological findings were identified via bladder biopsy. The associations between urinary biomarker levels and bladder conditions and histopathological findings were evaluated. The results reveal that patients with IC/BPS had significantly higher urinary MCP-1, eotaxin, TNF-α, PGE2, 8-OHdG, and 8-isoprostane levels than controls. Patients with Hunner’s IC (HIC) had significantly higher IL-8, CXCL10, BDNF, eotaxin, IL-6, MIP-1β, and RANTES levels than those with non-Hunner’s IC (NHIC). Patients with NHIC who had an MBC of ≤760 mL had significantly high urinary CXCL10, MCP-1, eotaxin, IL-6, MIP-1β, RANTES, PGE2, and 8-isoprostane levels and total antioxidant capacity. Patients with NHIC who had a higher glomerulation grade had significantly high urinary MCP-1, IL-6, RANTES, 8-OHdG, and 8-isoprostane levels. A significant association was observed between urinary biomarkers and glomerulation grade, MBC, VAS score, and bladder sensation. However, bladder-specific histopathological findings were not well correlated with urinary biomarker levels. The urinary biomarker levels can be useful for identifying HIC and different NHIC subtypes. Higher urinary inflammatory and oxidative stress biomarker levels are associated with IC/BPS. Most urinary biomarkers are not correlated with specific bladder histopathological findings; nevertheless, they are more important in the assessment of bladder condition than bladder histopathology.
Lipid Droplet Formation Is Regulated by Ser/Thr Phosphatase PPM1D via Dephosphorylation of Perilipin 1
Authors: Kamada Rui , Uno Sae , Kimura Nozomi , Yoshimura Fumihiko , Tanino Keiji , Sakaguchi Kazuyasu
Published: October 2022

Hypertrophy and hyperplasia of white adipocytes induce obesity, leading to diseases such as type 2 diabetes and hypertension, and even cancer. Hypertrophy of white adipocytes is attributed to the excessive storage of the energy form of triglycerides in lipid droplets (LDs). LDs are fat storage organelles that maintain whole-body energy homeostasis. It is important to understand the mechanism of LD formation for the development of obesity therapy; however, the regulatory mechanisms of LD size and formation are not fully understood. In this study, we demonstrated that the PPM family phosphatase PPM1D regulates LD formation. PPM1D specific inhibitor, SL-176 significantly decreased LD formation via two different pathways: dependent of and independent of adipocyte-differentiation processes. In the mature white adipocytes after differentiation, LD formation was found to be controlled by PPM1D via dephosphorylation of Ser511 of perilipin 1. We found that inhibition of PPM1D in mature white adipocytes significantly reduced the size of the LDs via dephosphorylation of Ser511 of perilipin 1 but did not change the lipolysis sensitivity and the total amount of lipid in cells. Collectively, the results of this study provide evidence that PPM1D plays an important role in LD formation in mature adipocytes.
Inflammatory and Prothrombotic Biomarkers, DNA Polymorphisms, MicroRNAs and Personalized Medicine for Patients with Peripheral Arterial Disease
Authors: Poredoš Pavel , Šabovič Mišo , Božič Mijovski Mojca , Nikolajević Jovana , Antignani Pier Luigi , Paraskevas Kosmas I. , Mikhailidis Dimitri P. , Blinc Aleš
Published: October 2022

Classical risk factors play a major role in the initiation and development of atherosclerosis. However, the estimation of risk for cardiovascular events based only on risk factors is often insufficient. Efforts have been made to identify biomarkers that indicate ongoing atherosclerosis. Among important circulating biomarkers associated with peripheral arterial disease (PAD) are inflammatory markers which are determined by the expression of different genes and epigenetic processes. Among these proinflammatory molecules, interleukin-6, C-reactive protein, several adhesion molecules, CD40 ligand, osteoprotegerin and others are associated with the presence and progression of PAD. Additionally, several circulating prothrombotic markers have a predictive value in PAD. Genetic polymorphisms significantly, albeit moderately, affect risk factors for PAD via altered lipoprotein metabolism, diabetes, arterial hypertension, smoking, inflammation and thrombosis. However, most of the risk variants for PAD are located in noncoding regions of the genome and their influence on gene expression remains to be explored. MicroRNAs (miRNAs) are single-stranded, noncoding RNAs that modulate gene expression at the post-transcriptional level. Patterns of miRNA expression, to some extent, vary in different atherosclerotic cardiovascular diseases. miRNAs appear to be useful in the detection of PAD and the prediction of progression and revascularization outcomes. In conclusion, taking into account one’s predisposition to PAD, i.e., DNA polymorphisms and miRNAs, together with circulating inflammatory and coagulation markers, holds promise for more accurate prediction models and personalized therapeutic options.
Natural Variation of Fatty Acid Desaturase Gene Affects Linolenic Acid Content and Starch Pasting Viscosity in Rice Grains
Authors: Zhang Liting , Xia Yu , Dong Yage , Xie Tianyi , Sun Wenqiang , Yu Sibin
Published: October 2022

Rice, as one of the main food crops, provides a vital source of dietary energy for over half the world’s population. The OsFAD3 gene encodes fatty acid desaturase, catalyzing the conversion of linoleic acid (LA) to alpha-linolenic acid (ALA) in rice. However, the genetic characterization of OsFAD3 and its role in the conversion of LA to ALA remains elusive. Here, we validated the effects of two homologous genes, OsFAD3-1 and OsFAD3-2, on the ALA and LA/ALA ratio in rice grains using near-isogenic lines. Two major haplotypes of OsFAD3-1 are identified with different effects on the ALA and LA/ALA ratio in rice germplasm. High expression of OsFAD3-1 is associated with high ALA accumulation and eating quality of rice grains. Overexpression of OsFAD3-1 driven by a seed-specific promoter increases the ALA content up to 16-fold in the endosperm. A diagnostic marker is designed based on an 8-bp insertion/deletion in the OsFAD3-1 promoter, which can recognize OsFAD3-1 alleles in rice. These results indicate that OsFAD3-1 is a useful target gene in marker-assisted breeding programs to improve varieties with high ALA and appropriate LA/ALA ratio in brown rice.