bluuebunny/crossref_metadata_2025 huggingface dataset:
Extractability of Curcuminoids Is Enhanced with Milk and Aqueous-Alcohol Mixtures
Authors: Kotha Raghavendhar R. , Tareq Fakir Shahidullah , Luthria Devanand L.
Published: August 2022

In this study, we evaluated the extractability of three curcuminoids (curcumin, demethoxycurcumin, and bisdemethoxycurcumin) from turmeric powder in several solvents using high-performance liquid chromatography (HPLC) with the diode-array detection method. These solvents include water, milk (homogenized, 2% reduced fat, low fat, fat free, soy, almond, coconut, and milkadamia), and aqueous ethanols (0%, 4%, 10%, 20%, 30%, 40%, 50%, and 100%). Ambient water was able to extract only 0.55 mg/g of curcuminoids, whereas warm water extracted more than four-fold higher amounts (2.42 mg/g). Almond, coconut, and milkadamia milk were able to extract only small amounts of curcuminoids at ambient temperatures (0.01–0.07 mg/g). The extractability of curcuminoids in these milk types did not improve, even in warm conditions (0.08–0.37 mg/g). Whereas dairy and soy milk extracted 6.76–9.75 mg/g of curcuminoids under ambient conditions, their extractability increased significantly in warm conditions by 30–100% higher (11.7–14.9 mg/g). The solubility of curcuminoids also varied remarkably in different proportions of aqueous-alcohol mixtures. With 4% ethanol, only 1.7 mg/g of curcuminoids were extracted, and the amounts improved with the increase in ethanol content up to 50% (32.2 mg/g), while 100% ethanol extracted a similar amount as 50% ethanol (34.2 mg/g). This study suggests that the extractability of curcuminoids from turmeric will be dependent on the type of diets consumed with the turmeric supplements.
Discovery of New Inhibitors of eEF2K from Traditional Chinese Medicine Based on In Silico Screening and In Vitro Experimental Validation
Authors: Fu Qinghua , Liu Xiaomei , Li Yan , Wang Peng , Wu Tian , Xiao Haihan , Zhao Yameng , Liao Qichao , Song Ziyi
Published: August 2022

Eukaryotic elongation factor 2 kinase (eEF2K) is a highly conserved α kinase and is increasingly considered as an attractive therapeutic target for cancer as well as other diseases. However, so far, no selective and potent inhibitors of eEF2K have been identified. In this study, pharmacophore screening, homology modeling, and molecular docking methods were adopted to screen novel inhibitor hits of eEF2K from the traditional Chinese medicine database (TCMD), and then cytotoxicity assay and western blotting were performed to verify the validity of the screen. Resultantly, after two steps of screening, a total of 1077 chemicals were obtained as inhibitor hits for eEF2K from all 23,034 compounds in TCMD. Then, to verify the validity, the top 10 purchasable chemicals were further analyzed. Afterward, Oleuropein and Rhoifolin, two reported antitumor chemicals, were found to have low cytotoxicity but potent inhibitory effects on eEF2K activity. Finally, molecular dynamics simulation, pharmacokinetic and toxicological analyses were conducted to evaluate the property and potential of Oleuropein and Rhoifolin to be drugs. Together, by integrating in silico screening and in vitro biochemical studies, Oleuropein and Rhoifolin were revealed as novel eEF2K inhibitors, which will shed new lights for eEF2K-targeting drug development and anticancer therapy.
Psycho-Neuro-Endocrine-Immunology: A Role for Melatonin in This New Paradigm
Authors: Bitzer-Quintero Oscar K. , Ortiz Genaro G. , Jaramillo-Bueno Socorro , Ramos-González Elsy J. , Márquez-Rosales María G. , Delgado-Lara Daniela L. C. , Torres-Sánchez Erandis D. , Tejeda-Martínez Aldo R. , Ramirez-Jirano Javier
Published: August 2022

Psychoneuroendocrinoimmunology is the area of study of the intimate relationship between immune, physical, emotional, and psychological aspects. This new way of studying the human body and its diseases was initiated in the last century’s first decades. However, the molecules that participate in the communication between the immune, endocrine, and neurological systems are still being discovered. This paper aims to describe the development of psychoneuroendocrinoimmunology, its scopes, limitations in actual medicine, and the extent of melatonin within it.
Metagenomics Approach to the Intestinal Microbiome Structure and Abundance in High-Fat-Diet-Induced Hyperlipidemic Rat Fed with (−)-Epigallocatechin-3-Gallate Nanoparticles
Authors: Chen Zhiyin , Liu Baogui , Gong Zhihua , Huang Hua , Gong Yihui , Xiao Wenjun
Published: August 2022

The effects of nanoparticles (NPs) on microbiota homeostasis and their physiological relevance are still unclear. Herein, we compared the modulation and consequent pharmacological effects of oral administration of (−)-epigallocatechin-3-gallate (EGCG)-loaded β-cyclodextrin (β-CD) NPs (EGCG@β-CD NPs) and EGCG on gut microbiota. EGCG@β-CD NPs were prepared using self-assembly and their influence on the intestinal microbiome structure was analyzed using a metagenomics approach. The “Encapsulation efficiency (EE), particle size, polydispersity index (PDI), zeta potential” of EGCG@β-CD NPs were recorded as 98.27 ± 0.36%, 124.6 nm, 0.313 and –24.3 mV, respectively. Surface morphology of EGCG@β-CD NPs was observed as spherical. Fourier-transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and molecular docking studies confirmed that EGCG could be well encapsulated in β-CD and formed as EGCG@β-CD NPs. After being continuously administered EGCG@β-CD NPs for 8 weeks, the serum cholesterol (TC), low-density lipoprotein cholesterol (LDL-C) and liver malondialdehyde (MDA) levels in the rats were significantly decreased, while the levels of catalase (CAT) and apolipoprotein-A1 (apo-A1) in the liver increased significantly in the hyperlipidemia model of rats, when compared to the high-fat-diet group. Furthermore, metagenomic analysis revealed that the ratio of Verrucomicrobia/Bacteroidetes was altered and Bacteroidetes decreased in the high-fat diet +200 mg/kg·bw EGCG@β-CD NPs group, while the abundance of Verrucomicrobia was significantly increased, especially Akkermansia muciniphila in rat feces. EGCG@β-CD NPs could be a promising EGCG delivery strategy to modulate the gut microbiota, enhancing its employment in the prevention of hyperlipidemia.
Chemical Composition, Antioxidant, Antibacterial, and Antibiofilm Activities of Backhousia citriodora Essential Oil
Authors: Lim Ann Chie , Tang Shirley Gee Hoon , Zin Noraziah Mohamad , Maisarah Abdul Mutalib , Ariffin Indang Ariati , Ker Pin Jern , Mahlia Teuku Meurah Indra
Published: August 2022

The essential oil of Backhousia citriodora, commonly known as lemon myrtle oil, possesses various beneficial properties due to its richness in bioactive compounds. This study aimed to characterize the chemical profile of the essential oil isolated from leaves of Backhousia citriodora (BCEO) and its biological properties, including antioxidant, antibacterial, and antibiofilm activities. Using gas chromatography–mass spectrometry, 21 compounds were identified in BCEO, representing 98.50% of the total oil content. The isomers of citral, geranial (52.13%), and neral (37.65%) were detected as the main constituents. The evaluation of DPPH radical scavenging activity and ferric reducing antioxidant power showed that BCEO exhibited strong antioxidant activity at IC50 of 42.57 μg/mL and EC50 of 20.03 μg/mL, respectively. The antibacterial activity results showed that BCEO exhibited stronger antibacterial activity against Gram-positive bacteria (Staphylococcus aureus and Staphylococcus epidermidis) than against Gram-negative bacteria (Escherichia coli and Klebsiella pneumoniae). For the agar disk diffusion method, S. epidermidis was the most sensitive to BCEO with an inhibition zone diameter of 50.17 mm, followed by S. aureus (31.13 mm), E. coli (20.33 mm), and K. pneumoniae (12.67 mm). The results from the microdilution method showed that BCEO exhibited the highest activity against S. epidermidis and S. aureus, with the minimal inhibitory concentration (MIC) value of 6.25 μL/mL. BCEO acts as a potent antibiofilm agent with dual actions, inhibiting (85.10% to 96.44%) and eradicating (70.92% to 90.73%) of the biofilms formed by the four tested bacteria strains, compared with streptomycin (biofilm inhibition, 67.65% to 94.29% and biofilm eradication, 49.97% to 89.73%). This study highlights that BCEO can potentially be a natural antioxidant agent, antibacterial agent, and antibiofilm agent that could be applied in the pharmaceutical and food industries. To the best of the authors’ knowledge, this is the first report, on the antibiofilm activity of BCEO against four common nosocomial pathogens.
Novel 1,3-Thiazole Analogues with Potent Activity against Breast Cancer: A Design, Synthesis, In Vitro, and In Silico Study
Authors: Salem Manar G. , El-Maaty Dina M. Abu , El-Deen Yassmina I. Mohey , Elesawy Basem H. , Askary Ahmad El , Saleh Asmaa , Saied Essa M. , Behery Mohammed El
Published: August 2022

Breast cancer is the most common cancer in women, responsible for over half a million deaths in 2020. Almost 75% of FDA-approved drugs are mainly nitrogen- and sulfur-containing heterocyclic compounds, implying the importance of such compounds in drug discovery. Among heterocycles, thiazole-based heterocyclic compounds have demonstrated a broad range of pharmacological activities. In the present study, a novel set of 1,3-thiazole derivatives was designed and synthesized based on the coupling of acetophenone derivatives, and phenacyl bromide was substituted as a key reaction step. The activity of synthesized compounds was screened against the proliferation of two breast cancer cell lines (MCF-7 and MDA-MB-231). Almost all compounds exhibited a considerable antiproliferative activity toward the breast cancer cells as compared to staurosporine, with no significant cytotoxicity toward the epithelial cells. Among the synthesized compounds, compound 4 exhibited the most potent antiproliferative activity, with an IC50 of 5.73 and 12.15 µM toward MCF-7 and MDA-MB-231 cells, respectively, compared to staurosporine (IC50 = 6.77 and 7.03 µM, respectively). Exploring the mechanistic insights responsible for the antiproliferative activity of compound 4 revealed that compound 4 possesses a significant inhibitory activity toward the vascular endothelial growth factor receptor-2 (VEGFR-2) with (IC50 = 0.093 µM) compared to Sorafenib (IC50 = 0.059 µM). Further, compound 4 showed the ability to induce programmed cell death by triggering apoptosis and necrosis in MCF-7 cells and to induce cell cycle arrest on MCF-7 cells at the G1 stage while decreasing the cellular population in the G2/M phase. Finally, detailed in silico molecular docking studies affirmed that this class of compounds possesses a considerable binding affinity toward VEGFR2 proteins. Overall, these results indicate that compound 4 could be a promising lead compound for developing potent anti-breast cancer compounds.
Layered Double Hydroxides as Rising-Star Adsorbents for Water Purification: A Brief Discussion
Authors: Gama Brígida Maria Villar da , Selvasembian Rangabhashiyam , Giannakoudakis Dimitrios A. , Triantafyllidis Konstantinos S. , McKay Gordon , Meili Lucas
Published: August 2022

Within the frame of this article, briefly but comprehensively, we present the existing knowledge, perspectives, and challenges for the utilization of Layered Double Hydroxides (LDHs) as adsorbents against a plethora of pollutants in aquatic matrixes. The use of LDHs as adsorbents was established by considering their significant physicochemical features, including their textural, structural, morphological, and chemical composition, as well as their method of synthesis, followed by their advantages and disadvantages as remediation media. The utilization of LDHs towards the adsorptive removal of dyes, metals, oxyanions, and emerging pollutants is critically reviewed, while all the reported kinds of interactions that gather the removal are collectively presented. Finally, future perspectives on the topic are discussed. It is expected that this discussion will encourage researchers in the area to seek new ideas for the design, development, and applications of novel LDHs-based nanomaterials as selective adsorbents, and hence to further explore the potential of their utilization also for analytic approaches to detect and monitor various pollutants.
Two New Phenolic Glycosides with Lactone Structural Units from Leaves of Ardisia crenata Sims with Antibacterial and Anti-Inflammatory Activities
Authors: Tao Huihui , Zhou Yongqiang , Yin Xin , Wei Xin , Zhou Ying
Published: August 2022

Two new lactones, named Ardisicreolides A–B (1–2), together with four known flavonoids, Quercetin (3), Myricetrin (4), Quercitrin (5), Tamarixetin 3-O-rhamnoside (6), were isolated from the ethyl acetate portion of 70% ethanol extracts of dried leaves from Ardisia crenata Sims. These compounds were identified from Ardisia crenata Sims for the first time. The structures of 1–6 were elucidated according to 1D and 2D-NMR methods and together with the published literature. All of the isolated compounds were evaluated for in vitro anti-microbial effect against Escherichia coli, Pseudomonas aeuroginosa, Enterococcus faecalis, Proteus vulgaris, Staphylococcus aureus, and Bacillus subtilis. In addition, compounds 1–2 were assessed for anti-inflammatory activity by acting on LPS-induced RAW 264.7 macrophage cells in vitro. The results showed that only compound 2 exhibited moderate antibacterial activity on Bacillus subtilis. Moreover, compounds 1 and 2 were found to significantly inhibit the production of nitric oxide (NO) and reduce the release of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-4 (IL-4), and interleukin-10 (IL-10) in LPS-induced RAW 264.7 macrophage cells. The present data suggest that lactones from the leaves of A. crenata Sims might be used as a potential source of natural anti-inflammatory agents.
Solution-Processed SnO2 Quantum Dots for the Electron Transport Layer of Flexible and Printed Perovskite Solar Cells
Authors: Kiani Muhammad Salman , Sadirkhanov Zhandos T. , Kakimov Alibek G. , Parkhomenko Hryhorii P. , Ng Annie , Jumabekov Askhat N.
Published: August 2022

Flexible and printed perovskite solar cells (PSCs) fabricated on lightweight plastic substrates have many excellent potential applications in emerging new technologies including wearable and portable electronics, the internet of things, smart buildings, etc. To fabricate flexible and printed PSCs, all of the functional layers of devices should be processed at low temperatures. Tin oxide is one of the best metal oxide materials to employ as the electron transport layer (ETL) in PSCs. Herein, the synthesis and application of SnO2 quantum dots (QDs) to prepare the ETL of flexible and printed PSCs are demonstrated. SnO2 QDs are synthesized via a solvothermal method and processed to obtain aqueous and printable ETL ink solutions with different QD concentrations. PSCs are fabricated using a slot-die coating method on flexible plastic substrates. The solar cell performance and spectral response of the obtained devices are characterized using a solar simulator and an external quantum efficiency measurement system. The ETLs prepared using 2 wt% SnO2 QD inks are found to produce devices with a high average power conversion efficiency (PCE) along with a 10% PCE for a champion device. The results obtained in this work provide the research community with a method to prepare fully solution-processed SnO2 QD-based inks that are suitable for the deposition of SnO2 ETLs for flexible and printed PSCs.
Theory of MOCVD Growth of III-V Nanowires on Patterned Substrates
Authors: Dubrovskii Vladimir G.
Published: August 2022

An analytic model for III-V nanowire growth by metal organic chemical vapor deposition (MOCVD) in regular arrays on patterned substrates is presented. The model accounts for some new features that, to the author’s knowledge, have not yet been considered. It is shown that MOCVD growth is influenced by an additional current into the nanowires originating from group III atoms reflected from an inert substrate and the upper limit for the group III current per nanowire given by the total group III flow and the array pitch. The model fits the data on the growth kinetics of Au-catalyzed and catalyst-free III-V nanowires quite well and should be useful for understanding and controlling the MOCVD nanowire growth in general.
HPV-Related Skin Phenotypes in Patients with Inborn Errors of Immunity
Authors: El Kettani Assiya , Ailal Fatima , El Bakkouri Jalila , Zerouali Khalid , Béziat Vivien , Jouanguy Emmanuelle , Casanova Jean-Laurent , Bousfiha Ahmed Aziz
Published: August 2022

Patients with inborn errors of immunity (IEI) are prone to develop infections, either due to a broad spectrum of pathogens or to only one microbe. Since skin is a major barrier tissue, cutaneous infections are among the most prevalent in patients with IEI due to high exposures to many microbes. In the general population, human papillomaviruses (HPVs) cause asymptomatic or self-healing infections, but, in patients with IEI, unusual clinical expression of HPV infection is observed ranging from epidermodysplasia verruciformis (EV) (a rare disease due to β-HPVs) to profuse, persistent, and recalcitrant warts (due to α-, γ-, and μ-HPVs) or even tree man syndrome (due to HPV2). Mutations in EVER1, EVER2, and CIB1 are associated with EV phenotype; GATA2, CXCR4, and DOCK8 mutations are typically associated with extensive HPV infections, but there are several other IEI that are less frequently associated with severe HPV lesions. In this review, we describe clinical, immunological, and genetic patterns of IEI related to severe HPV cutaneous infections and propose an algorithm for diagnosis of IEI with severe warts associated, or not, with lymphopenia.
Direct and Rapid Identification of Vibrio Cholerae Serogroup and Toxigenicity by a Novel Multiplex Real-Time Assay
Authors: Yan Yong , Zhan Li , Zhu Guoying , Zhang Junyan , Li Ping , Chen Lixia , He Peiyan , Luo Jianyong , Chen Zhongwen
Published: August 2022

Molecular diagnostic assays for cholera detection have superior sensitivity to conventional assays and are now being accepted as the new standard method, especially the real-time PCR/RT-PCR. However, limited throughput capacity and long detection duration prevent them from detecting more specimens and more targets in one turnaround time simultaneously. In this study, we utilized nucleic acid extraction-free, direct RT-PCR and high-speed amplification to develop a novel multiplex assay, a quadplex direct one-tube real-time RT-PCR assay, for rapid detection of the serogroup and cholera toxin toxigenicity of Vibrio cholerae targeting the epsM, ctxA, rfb-O1, and rfb-O139 genes. Performance of the multiplex assay was evaluated by comparison with the monoplex real-time PCR assay according to the China Cholera Prevention Manual. Detection data from clinical specimens showed that the new assay had good diagnostic sensitivities for epsM (100%, n = 301), ctxA (100%, n = 125), rfb-O1 (100%, n = 85), and rfb-O139 (97.87%, n = 49). Analysis of the analytical sensitivities with serial dilutions of positive standards showed that the detection limits of the new assay for Vibrio cholerae epsM,ctxA,rfb-O1, and rfb-O139 were up to 200, 590, 115, and 1052 copies per mL lower than the monoplex real-time PCR (910, 345, and 1616 copies/mL respectively, for ctxA,rfb-O1, and rfb-O139). The results indicate that the multiplex assay is a rapid, sensitive, specific, and easy-to-use detection tool for Vibrio cholerae, especially suitable for rapid identification and screening detection of mass specimens.
Epidemic Characteristics of HIV Drug Resistance in Hefei, Anhui Province
Authors: Zheng Shan , Wu Jianjun , Hao Jingjing , Wang Dong , Hu Zhongwang , Liu Lei , Song Chang , Hu Jing , Lei Yanhua , Wang Hai , Liao Lingjie , Feng Yi , Shao Yiming , Ruan Yuhua , Xing Hui
Published: August 2022

To study the characteristics of HIV pretreatment drug resistance (PDR) and acquired drug resistance (ADR) in Hefei, a cross-sectional survey was used to collect 816 samples from newly reported HIV infections from 2017 to 2020 and 127 samples from HIV infections with virological failure from 2018 to 2019 in Hefei. HIV drug resistance levels and drug resistance mutations were interpreted using the Stanford Drug Resistance Database. Molecular networks were constructed by HIV-TRACE. Among the newly reported infections in Hefei, the prevalence of PDR was 6.4% (52/816). The drug resistance mutations were mainly V179E/D/T (12.4%), K103N (1.3%), and V106I/M (1.3%). In addition, it was found that the CRF55_01B subtype had a higher drug resistance rate than other subtypes (p < 0.05). Molecular network analysis found that K103N and V179E may be transmitted in the cluster of the CRF55_01B subtype. The prevalence of ADR among HIV infections with virological failure was 38.6% (49/127), and the drug resistance mutations were mainly M184V (24.4%), K103N/S (15.7%), Y181C (11.0%), G190S/A/E (10.2%), and V106M/I (10.2%). The molecular network was constructed by combining HIV infections with virological failure and newly reported infections; M184V and Y181C may be transmitted between them. The chi-square trend test results indicated that the higher the viral load level, the greater the number of newly reported infections linked to the infections with virological failure in the molecular network. In conclusion, interventions should focus on infections of the CRF55_01B subtype to reduce the transmission of drug-resistant strains. However, improving the treatment effect of HIV infections is beneficial for reducing the second-generation transmission of HIV.
Effect of Landscape Composition and Invasive Plants on Pollination Networks of Smallholder Orchards in Northeastern Thailand
Authors: Simla Pattraporn , Chaianunporn Thotsapol , Sankamethawee Wangworn , Hughes Alice C. , Sritongchuay Tuanjit
Published: August 2022

Destruction of natural habitat, land-use changes and biological invasion are some of the major threats to biodiversity. Both habitat alteration and biological invasions can have impacts on pollinator communities and pollination network structures. This study aims to examine the effect of an invasive plant, praxelis (Praxelis clematidea; Asteraceae), and land-use types on pollinator communities and the structure of pollination networks. We conducted the study in smallholder orchards which are either invaded or non-invaded by P. clematidea. We estimated the pollinator richness, visitation rates, and pollinator diversity and evaluated the network structures from 18 smallholder orchards in Northeastern Thailand. The effect of landscape structure in the vicinity of the orchards was investigated, with the proportion of agricultural, forest, and urban landscape within a 3 km radius analyzed. The invasive species and land-use disturbance influence the pollinator communities and pollination network structure at species level was affected by the presence of P. clematidea. Bees were the most important pollinator group for pollinator communities and pollination networks of both invaded or non-invaded plots, as bees are a generalist species, they provide the coherence of both the network and its own module. The urban landscape had a strong negative influence on pollinator richness, while the proportions of agriculture and forest landscape positively affected the pollinator community.
A Proposed New Species Complex within the Cosmopolitan Ring Nematode Criconema annuliferum (de Man, 1921) Micoletzky, 1925
Authors: Clavero-Camacho Ilenia , Palomares-Rius Juan Emilio , Cantalapiedra-Navarrete Carolina , Castillo Pablo , Liébanas Gracia , Archidona-Yuste Antonio
Published: August 2022

Ring nematodes are obligate ectoparasites on cultivated and wild herbaceous and woody plants, inhabiting many types of soil, but particularly sandy soils. This study explored the morphometrical and molecular diversity of ring nematodes resembling Criconema annuliferum in 222 soil samples from fruit crops in Spain, including almond, apricot, peach and plum, as well as populations from cultivated and wild olives, and common yew. Ring nematodes of the genus Criconema were detected in 12 samples from under Prunus spp. (5.5%), showing a low to moderate nematode soil densities in several localities from southeastern and northeastern Spain. The soil population densities of Criconema associated with Prunus spp. ranged from 1 nematode/500 cm3 of soil in apricot at Sástago (Zaragoza province) to 7950 and 42,491 nematodes/500 cm3 of soil in peach at Ricla and Calasparra (Murcia province), respectively. The integrative taxonomical analyses reveal the presence of two cryptic species identified using females, males (when available), and juveniles with detailed morphology, morphometry, and molecular markers (D2-D3, ITS, 18S, and COI), described herein as Criconema paraannuliferum sp. nov. and Criconema plesioannuliferum sp. nov. All molecular markers from each species were obtained from the same individuals, and these individuals were also used for morphological and morphometric analyses. Criconema paraannuliferum sp. nov. was found in a high soil density in two peach fields (7950 and 42,491 nematodes/500 cm3 of soil) showing the possibility of being pathogenic in some circumstances.
Seed Dormancy and Seedling Ecophysiology Reveal the Ecological Amplitude of the Threatened Endemism Picris willkommii (Schultz Bip.) Nyman (Asteraceae)
Authors: Fernández Manuel , Tapias Raúl
Published: August 2022

Plant communities can undergo drastic changes in their composition if the ecosystem is severely altered by human actions or climate change. These changes endanger any vulnerable species, mainly if it lives in a small area, as is the case of Picris willkommii (Schultz Bip.) Nyman. Therefore, it is essential to know how an ecosystem alteration could affect the seasonal pattern of the life cycle, seed production, germination time, as well as both plant emergence and development. During three consecutive years, the growth phenology and seed morpho-physiological traits of Picris willkommii were assessed, as well as the environmental factors that affect them (light, temperature, substrate). Under natural conditions, germination is in early autumn (15–25 °C air temperature), flowering is in spring, and seed maturation in late spring. The species produces two types of seeds differentiated in the degree of dormancy and other morpho–physiological traits, which contributes to the dispersal and spreading capacity; it prefers fine-textured limestone substrates with high N and P availability; it does not tolerate frosts below −5 °C; and it is able to acclimatize to changing environmental conditions, but there is a risk of being replaced by other more aggressive species. All of this is useful for species conservation programs.
Seed Priming with Carbon Nanomaterials Improves the Bioactive Compounds of Tomato Plants under Saline Stress
Authors: González-García Yolanda , López-Vargas Elsy Rubisela , Pérez-Álvarez Marissa , Cadenas-Pliego Gregorio , Benavides-Mendoza Adalberto , Valdés-Reyna Jesús , Pérez-Labrada Fabián , Juárez-Maldonado Antonio
Published: August 2022

The consumption of food with a high content of bioactive compounds is correlated with the prevention of chronic degenerative diseases. Tomato is a food with exceptional nutraceutical value; however, saline stress severely affects the yield, the quality of fruits, and the agricultural productivity of this crop. Recent studies have shown that seed priming can mitigate or alleviate the negative effects caused by this type of stress. However, the use of carbon nanomaterials (CNMs) in this technique has not been tested for this purpose. In the present study, the effects of tomato seed priming with carbon nanotubes (CNTs) and graphene (GP) (50, 250, and 500 mg L−1) and two controls (not sonicated and sonicated) were evaluated based on the content of photosynthetic pigments in the leaves; the physicochemical parameters of the fruits; and the presence of enzymatic and non-enzymatic antioxidant compounds, carotenoids, and stress biomarkers such as hydrogen peroxide (H2O2) and malondialdehyde (MDA) in the leaves and fruits of tomato plants without saline stress and with saline stress (50 mM NaCl). The results show that saline stress in combination with CNTs and GP increased the content of chlorophylls (9.1–21.7%), ascorbic acid (19.5%), glutathione (≈13%), proteins (9.9–11.9%), and phenols (14.2%) on the leaves. The addition of CNTs and GP increased the activity of enzymes (CAT, APX, GPX, and PAL). Likewise, there was also a slight increase in the content of H2O2 (by 20.5%) and MDA (3.7%) in the leaves. Salinity affected the quality of tomato fruits. The physico-chemical parameters and bioactive compounds in both the stressed and non-stressed tomato plants were modified with the addition of CNTs and GP. Higher contents of total soluble solids (25.9%), phenols (up to 144.85%), flavonoids (up to 37.63%), ascorbic acid (≈28%), and lycopene (12.4–36.2%) were observed. The addition of carbon nanomaterials by seed priming in tomato plants subjected to saline stress modifies the content of bioactive compounds in tomato fruits and improves the antioxidant defense system, suggesting possible protection of the plant from the negative impacts of stress by salinity. However, analysis of the mechanism of action of CNMs through seed priming, in greater depth is suggested, perhaps with the use of omics sciences.
Seed-to-Seedling Transition: Novel Aspects
Authors: Smolikova Galina , Medvedev Sergei
Published: August 2022

Transition from seed to seedling represents a critical stage in plants’ life cycles. This process includes three significant events in the seeds: (i) tissue hydration, (ii) the mobilization of reserve nutrients, and (iii) the activation of metabolic activity. Global metabolic rearrangements lead to the initiation of radicle growth and the resumption of vegetative development. It requires massive reprogramming of the transcriptome, proteome, metabolome, and attendant signaling pathways, resulting in the silencing of seed-maturation genes and the activation of vegetative growth genes. This Special Issue discusses the mechanisms of genetic, epigenetic, and hormonal switches during seed-to-seedling transitions. Detailed information has also been covered regarding the influence of germination features on seedling establishment.
Azadirachta indica A. Juss Fruit Mesocarp and Epicarp Extracts Induce Antimicrobial and Antiproliferative Effects against Prostate (PC-3), Breast (MCF-7), and Colorectal Adenocarcinoma (Caco-2) Cancer Cell Lines through Upregulation of Proapoptotic Genes
Authors: Ibrahim Omer H. M. , Mousa Magdi A. A. , Asiry Khalid A. , Alhakamy Nabil A. , Abo-Elyousr Kamal A. M.
Published: August 2022

Effective alternative strategies and methodological approaches are critically necessary for cancer prevention and therapy. In this study, we investigated the antitumor potential of neem fruit mesocarp and epicarp extracts. The chemical composition of the derived extracts was characterized using GC–MS. Data were collected on the antimicrobial activity of the extracts in addition to the cytotoxicity effect evaluated against PC-3, MCF-7, and Caco-2 cancer cell lines, compared with the normal Vero cells. Cell-cycle arrest, apoptosis, and expression of apoptosis-related genes were assessed on PC-3 cells. Both extracts had significant antiproliferative effects on all tested cell lines in a dose-dependent manner, with the mesocarp extract being more potent. Both extracts also showed high antibacterial and antifungal activities. These results were related to the chemical constituents of the extracts identified by the GC–MS analysis. The extract of neem fruit mesocarp caused cell-cycle arrest at G2/M phase of PC-3 cells. The cytotoxicity of neem mesocarp extract is strongly correlated with the induction of apoptosis, where it caused downregulation of the antiapoptotic BCL2 gene but upregulation of the proapoptotic P53 and BAX genes. This study showed that neem fruit extract is potential anticancer material in the future.
The Role of Transcription Factors in the Regulation of Plant Shoot Branching
Authors: Zhang Lingling , Fang Weimin , Chen Fadi , Song Aiping
Published: August 2022

Transcription factors, also known as trans-acting factors, balance development and stress responses in plants. Branching plays an important role in plant morphogenesis and is closely related to plant biomass and crop yield. The apical meristem produced during plant embryonic development repeatedly produces the body of the plant, and the final aerial structure is regulated by the branching mode generated by axillary meristem (AM) activities. These branching patterns are regulated by two processes: AM formation and axillary bud growth. In recent years, transcription factors involved in regulating these processes have been identified. In addition, these transcription factors play an important role in various plant hormone pathways and photoresponses regulating plant branching. In this review, we start from the formation and growth of axillary meristems, including the regulation of hormones, light and other internal and external factors, and focus on the transcription factors involved in regulating plant branching and development to provide candidate genes for improving crop architecture through gene editing or directed breeding.
Development of Antibody-like Proteins Targeting the Oncogenic Ser/Thr Protein Phosphatase PPM1D
Authors: Ikeura Megumi , Tashiro Hiroto , Yamagata Yuka , Saito Hikaru , Kobayashi Tamaki , Mizunuma Masataka , Yamazaki Kazuki , Baba Keisuke , Furukawa Kazuhiro , Chuman Yoshiro
Published: August 2022

PPM1D, a protein Ser/Thr phosphatase, is overexpressed in various cancers and functions as an oncogenic protein by inactivating the p53 pathway. Therefore, molecules that bind PPM1D are expected to be useful anti-cancer agents. In this study, we constructed a phage display library based on the antibody-like small molecule protein adnectin and screened for PPM1D-specific binding molecules. We identified two adnectins, PMDB-1 and PMD-24, that bind PPM1D specific B-loop and PPM1D430 as targets, respectively. Specificity analyses of these recombinant proteins using other Ser/Thr protein phosphatases showed that these molecules bind to only PPM1D. Expression of PMDB-1 in breast cancer-derived MCF-7 cells overexpressing endogenous PPM1D stabilized p53, indicating that PMDB-1 functions as an inhibitor of PPM1D. Furthermore, MTT assay exhibited that MCF-7 cells expressing PMDB-1 showed inhibition of cell proliferation. These data suggest that the adnectin PMDB-1 identified in this study can be used as a lead compound for anti-cancer drugs targeting intracellular PPM1D.
Reconsidering Homosexual Unification in Islam: A Revisionist Analysis of Post-Colonialism, Constructivism and Essentialism
Authors: Zaharin Aisya Aymanee M.
Published: August 2022

While there are Muslims who hold that the two identities Muslim and homosexual are mutually exclusive and that it is illegal to practise homosexual acts, the ongoing Muslim revisionist movement seems to provide a more understanding approach. Members of this movement argue that although the act of homosexuality is mentioned in the Qur’ān, this term is not inherently similar to the contemporary understanding of homosexuality based on love (mawaddah) and mutual consent. This paper provides a comprehensive understanding of theological, historical and sociological discourses to demonstrate some of the challenges facing contemporary sexual ethics in relation to Muslim homosexuality and its relationship to power (religious, patriarchal and neo-colonialist). It argues that conservative and centrist scholars have always presented homosexuality based on cis-hetero morality standards, but not from a deep understanding of the Qur’ān and the Hadith. It also argues that from a conservative view, homosexuality has never been addressed from either a medical/psychological (essentialism) or a social constructivist perspective, thereby further denying the existence of men who are not attracted to females as explained in the Qur’ān (24:31). On a historical level, this paper offers a discussion of legal, social and political genealogies of the history of same-sex attraction among Muslims by addressing relevant questions related to pre-colonial and post-colonial legacies. This paper then considers sociological and scientific approaches (constructivism and essentialism theories) that explain a place for same-sex unions in Islam.
Religious Belief through Drum-Sound Experience: Bengal’s Devotional Dialectic of the Classical Goddess and Indigenous God
Authors: Sarbadhikary Sukanya
Published: August 2022

The epistemic question about what constitutes religious belief in non-Western contexts is addressed here through the ontology of sonic experience. I demonstrate that religious beliefs are habitually ingrained as long-sustaining visceral memories, when afforded by sensory—for instance, aural—affects. Bengal’s peculiar devotional milieu constructs a prototype of oppositions. On one end is the urban, classical, martial goddess, Durga, with elite histories of acquiring a high Brahmanical form, and whose autumnal rituals are based on scriptural rules, caste hierarchies, and distance among the devotees and deity. On the other end is the rural, indigenous, non-classical, peasant god, Shiva, whose spring-time worship celebrating primordial death and regeneration is based on intensely embodied and communitarian principles of identity among the caste-equal bodies of devotee men, and even their god. Based on immersive ethnographic analyses, the paper argues that these dual psychological ends of the regional sacred cosmos are made vividly real through differential perceptive experiences of percussion sounds (ubiquitous in these festivities), their varied tempos, textures, volumes, and rhythm modulations. Through phenomenological deep listening, I describe stark styles of making and playing the sacred membranophone drum, dhak, which embodies distinct rhythm styles, relationships with rituals, and psychophysical effects on the devotional ensembles. I show how the bodies of devotees, dhak players (dhakis), deities, and even the dhak, become tied to the tonalities of the drum, which is taught through generations of deft learning among dhakis, to sound distinctly when echoed for Durga and Shiva. The paper’s main argument is that these dhak sounds, which have remained a conceptual oversight in literature, not only aid in, but indeed, enable the experience of and belief in Bengal’s divergent deities. It is through such empowering sensory sedimentations of the different sounds of the same percussion, that people recognize, remember, and maintain the region’s devotional dialectic and complex religious lifeworld. In essence, the body’s powerful experiences of drum sounds make religious belief palpable and possible.
Fuzzy-Based Privacy-Preserving Scheme of Low Consumption and High Effectiveness for IoTs: A Repeated Game Model
Authors: Cao Laicheng , Zhu Min
Published: August 2022

In the Internet of things (IoTs), data transmission via network coding is highly vulnerable to intra-generation and inter-generation pollution attacks. To mitigate such attacks, some resource-intensive privacy-preserving schemes have been adopted in the previous literature. In order to balance resource consumption and data-privacy-preserving issues, a novel fuzzy-based privacy-preserving scheme is proposed. Our scheme is constructed on a T-S fuzzy trust theory, and network coding data streams are routed in optimal clusters formulated by a designed repeated game model to defend against pollution attacks. In particular, the security of our scheme relies on the hardness of the discrete logarithm. Then, we prove that the designed repeated game model has a subgame-perfect Nash equilibrium, and the model can improve resource utilization efficiency under the condition of data security. Simulation results show that the running time of the proposed privacy-preserving scheme is less than 1 s and the remaining energy is higher than 4 J when the length of packets is greater than 400 and the number of iterations is 100. Therefore, our scheme has higher time and energy efficiency than those of previous studies. In addition, the effective trust cluster formulation scheme (ETCFS) can formulate an optimal cluster more quickly under a kind of camouflage attack.
An Energy-Efficient Clustering Method for Target Tracking Based on Tracking Anchors in Wireless Sensor Networks
Authors: Qu Zhiyi , Li Baoqing
Published: August 2022

As a key technology in wireless sensor networks (WSNs), target tracking plays an essential role in many applications. To improve energy efficiency, clustering is widely used in tracking to organize the network to achieve data fusion and reduce communication costs. Many existing studies make dynamic adjustments based on static clusters to track moving targets. However, the additional overhead caused by frequent cluster reconstruction and redundant data transmission is rarely considered. To address this issue, we propose a tracking-anchor-based clustering method (TACM) in this paper, in which tracking anchors are introduced to provide activation indications for sensors according to the target position. We use the rough fuzzy C-means (RFCM) algorithm to locate the anchors and use the membership table to activate sensors to form a cluster. Since there are no sending, receiving, and fusing data tasks for anchors, they are lightly burdened and can significantly reduce the frequency of being rotated. Moreover, the state of cluster members (CMs) is scheduled using the linear 0–1 programming to reduce redundant transmissions. The simulation results demonstrate that, compared with some existing clustering methods, the proposed TACM effectively reduces the energy consumption when tracking a moving target, thus prolonging the network lifetime.
SASMOTE: A Self-Attention Oversampling Method for Imbalanced CSI Fingerprints in Indoor Positioning Systems
Authors: Liu Ankang , Cheng Lingfei , Yu Changdong
Published: August 2022

WiFi localization based on channel state information (CSI) fingerprints has become the mainstream method for indoor positioning due to the widespread deployment of WiFi networks, in which fingerprint database building is critical. However, issues, such as insufficient samples or missing data in the collection fingerprint database, result in unbalanced training data for the localization system during the construction of the CSI fingerprint database. To address the above issue, we propose a deep learning-based oversampling method, called Self-Attention Synthetic Minority Oversampling Technique (SASMOTE), for complementing the fingerprint database to improve localization accuracy. Specifically, a novel self-attention encoder-decoder is firstly designed to compress the original data dimensionality and extract rich features. The synthetic minority oversampling technique (SMOTE) is adopted to oversample minority class data to achieve data balance. In addition, we also construct the corresponding CSI fingerprinting dataset to train the model. Finally, extensive experiments are performed on different data to verify the performance of the proposed method. The results show that our SASMOTE method can effectively solve the data imbalance problem. Meanwhile, the improved location model, 1D-MobileNet, is tested on the balanced fingerprint database to further verify the excellent performance of our proposed methods.
Enhanced Laser-Induced Breakdown Spectroscopy for Heavy Metal Detection in Agriculture: A Review
Authors: Yang Zihan , Ren Jie , Du Mengyun , Zhao Yanru , Yu Keqiang
Published: August 2022

Heavy metal pollution in agriculture is a significant problem that endangers human health. Laser-induced breakdown spectroscopy (LIBS) is an emerging technique for material and elemental analysis, especially heavy metals, based on atomic emission spectroscopy. The LIBS technique has been widely used for rapid detection of heavy metals with its advantages of convenient operation, simultaneous detection of multi-elements, wide range of elements, and no requirement for the state and quantity of samples. However, the development of LIBS is limited by its detection sensitivity and limit of detection (LOD). Therefore, in order to improve the detection sensitivity and LOD of LIBS, it is necessary to enhance the LIBS signal to achieve the purpose of detecting heavy metal elements in agriculture. This review mainly introduces the basic instruments and principles of LIBS and summarizes the methods of enhanced LIBS signal detection of heavy metal elements in agriculture over the past 10 years. The three main approaches to enhancing LIBS are sample pretreatment, adding laser pulses, and using auxiliary devices. An enhanced LIBS signal may improve the LOD of heavy metal elements in agriculture and the sensitivity and stability of the LIBS technique. The enhanced LIBS technique will have a broader prospect in agricultural heavy metal monitoring and can provide technical support for developing heavy metal detection instruments.
Advances in Vision-Based Gait Recognition: From Handcrafted to Deep Learning
Authors: Mogan Jashila Nair , Lee Chin Poo , Lim Kian Ming
Published: August 2022

Identifying people’s identity by using behavioral biometrics has attracted many researchers’ attention in the biometrics industry. Gait is a behavioral trait, whereby an individual is identified based on their walking style. Over the years, gait recognition has been performed by using handcrafted approaches. However, due to several covariates’ effects, the competence of the approach has been compromised. Deep learning is an emerging algorithm in the biometrics field, which has the capability to tackle the covariates and produce highly accurate results. In this paper, a comprehensive overview of the existing deep learning-based gait recognition approach is presented. In addition, a summary of the performance of the approach on different gait datasets is provided.