bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of Chemogenetic Inhibition of D1 or D2 Receptor-Containing Neurons of the Substantia Nigra and Striatum in Mice With Tourette Syndrome
Authors: Lin Lixue , Lan Yuye , Zhu He , Yu Lingling , Wu Shuang , Wan Wangyixuan , Shu Yang , Xiang Hongchun , Hou Tengfei , Zhang Hong , Ma Yan , Su Wen , Li Man
Published: December 2021

As tourette syndrome (TS) is a common neurobehavioral disorder, the primary symptoms of which include behavioral stereotypies. Dysfunction of the substantia nigra–striatum network could be the main pathogenesis of TS, which is closely associated with dopamine (DA) and its receptors. TS is often resistant to conventional treatments. Therefore, it is necessary to investigate the neurobiological mechanisms underlying its pathogenesis. In this study, we investigated whether chemogenetic activation or inhibition of dopaminergic D1 receptor (D1R)- or D2 receptor (D2R)-containing neurons in the substantia nigra pars compacta (SNpc) or dorsal striatum (dSTR) affected the stereotyped behavior and motor functions of TS mice. Intraperitoneal injection of 3,3′-iminodipropionitrile (IDPN) was used to induce TS in mice. Stereotyped behavior test and open-field, rotarod, and grip strength tests were performed to evaluate stereotyped behavior and motor functions, respectively. Immunofluorescence labeling was used to detect the co-labeling of virus fluorescence and D1R or D2R. We found that chemogenetic inhibition of D1R- or D2R-containing neurons in the SNpc and dSTR alleviated behavioral stereotypies and motor functions in TS mice. Chemogenetic activation of D1R-containing neurons in the dSTR aggravated behavioral stereotypies and motor functions in vehicle-treated mice, but neither was aggravated in TS mice. In conclusion, chemogenetic inhibition of D1R- or D2R-containing neurons in the SNpc and dSTR alleviated behavioral stereotypies of TS, providing a new treatment target for TS. Moreover, the activation of D1R-containing neurons in the dSTR may contribute to the pathogenesis of TS, which can be chosen as a more precise target for treatment.
EEG-Microstates Reflect Auditory Distraction After Attentive Audiovisual Perception Recruitment of Cognitive Control Networks
Authors: Korn Ute , Krylova Marina , Heck Kilian L. , Häußinger Florian B. , Stark Robert S. , Alizadeh Sarah , Jamalabadi Hamidreza , Walter Martin , Galuske Ralf A. W. , Munk Matthias H. J.
Published: December 2021

Processing of sensory information is embedded into ongoing neural processes which contribute to brain states. Electroencephalographic microstates are semi-stable short-lived power distributions which have been associated with subsystem activity such as auditory, visual and attention networks. Here we explore changes in electrical brain states in response to an audiovisual perception and memorization task under conditions of auditory distraction. We discovered changes in brain microstates reflecting a weakening of states representing activity of the auditory system and strengthening of salience networks, supporting the idea that salience networks are active after audiovisual encoding and during memorization to protect memories and concentrate on upcoming behavioural response.
In vitro Prebiotic Properties of Garlic Polysaccharides and Its Oligosaccharide Mixtures Obtained by Acid Hydrolysis
Authors: Lu Xiaoming , Li Ningyang , Zhao Renjie , Zhao Meng , Cui Xuanxuan , Xu Yukun , Qiao Xuguang
Published: December 2021

Fructans and oligofructose are usually used as prebiotics without any limitation in functional food or food ingredients. The degree of polymerization (DP) of polysaccharides affects the utilization of probiotics. Garlic is rich in fructans. The objective of this study was to extract and purify polysaccharides from garlic, analyze its composition, hydrolyze them using HCl, and then evaluate the prebiotic potential of the garlic neutral polysaccharides (GPs) before and after hydrolysis. GPs were 6.57 × 103 Da with a composition of fructose and glucose at a ratio of 4:1. After acid hydrolysis, low molecular weight fraction in garlic oligofructose (GOs) may be eliminated through ultrafiltration. The content of oligosaccharides with an average DP < 10 increased from 15 to 75%. GPs and GOS had a stronger resistance to acid conditions in human stomach than fructooligosaccharide, and GOs showed better prebiotic properties on the growth of lactobacilli than GPs. This study evaluates the prebiotic potential of the garlic frutctans and oligosaccharides mixtures obtained by acid hydrolysis, which may be used as an ingredient in functional food and nutraceutical products.
Frozen Section Analysis and Real-Time Magnetic Resonance Imaging of Surgical Specimen Oriented on 3D Printed Tongue Model to Assess Surgical Margins in Oral Tongue Carcinoma: Preliminary Results
Authors: Giannitto Caterina , Mercante Giuseppe , Disconzi Luca , Boroni Riccardo , Casiraghi Elena , Canzano Federica , Cerasuolo Michele , Gaino Francesca , De Virgilio Armando , Fiamengo Barbara , Ferreli Fabio , Esposito Andrea Alessandro , Oliva Paolo , Ronzoni Flavio , Terracciano Luigi , Spriano Giuseppe , Balzarini Luca
Published: December 2021

Background

A surgical margin is the apparently healthy tissue around a tumor which has been removed. In oral cavity carcinoma, a negative margin is considered ≥ 5 mm, a close margin between 1 and 5 mm, and a positive margin ≤ 1 mm. Currently, the intraoperative surgical margin status is based on the visual inspection and tissue palpation by the surgeon and intraoperative histopathological assessment of the resection margins by frozen section analysis (FSA). FSA technique is limited and susceptible to sampling errors. Definitive information on the deep resection margins requires postoperative histopathological analysis.

Methods

We described a novel approach for the assessment of intraoperative surgical margins by examining a surgical specimen oriented through a 3D-printed specific patient tongue with real-time Magnetic Resonance Imaging (MRI). We reported the preliminary results of a case series of 10 patients, prospectively enrolled, with oral tongue carcinoma who underwent surgery between February 2020 and April 2021. Two radiologists with 5 and 10 years of experience, respectively, in Head and Neck radiology in consensus evaluated specimen MRI and measured the distance between the tumor and the specimen surface. We performed intraoperative bedside FSA. To compare the performance of bedside FSA and MRI in predicting definitive margin status we computed the weighted sensitivity (SE), specificity (SP), accuracy (ACC), area under the ROC curve (AUC), F1-score, Positive Predictive Value (PPV), and Negative Predictive Value (NPV). To express the concordance between FSA and ex-vivo MRI we reported the jaccard index.

Results

Intraoperative bedside FSA showed SE of 90%, SP of 100%, F1 of 95%, ACC of 0.9%, PPV of 100%, NPV (not a number), and jaccard of 90%, and ex-vivo MRI showed SE of 100%, SP of 100%, F1 of 100%, ACC of 100%, PPV of 100%, NPV of 100%, and jaccard of 100%. These results needed to be validated in a larger sample size of 21- 44 patients.

Conclusion

The presented method allows a more accurate evaluation of surgical margin status, and the first clinical experiences underline the high potential of integrating FSA with ex-vivo MRI of the fresh surgical specimen.
Clinical and Functional Characterization of a Novel Mutation in AVPR2 Causing Nephrogenic Diabetes Insipidus in a Four-Generation Chinese Family
Authors: Guo Shusen , Wu Shimin , Li Zhuxi , Huang Lianjing , Zhan Di , Zhang Cai , Luo Xiaoping
Published: December 2021

Background: Congenital nephrogenic diabetes insipidus (CNDI) is a rare inherited disease that is caused by mutations in arginine vasopressin receptor 2 (AVPR2) or aquaporin 2 (AQP2). Functional analysis of the mutated receptor is necessary to verify the impact of the mutation on receptor function and suggest some possible therapeutic strategies for specific functional defects.

Methods: Family history and clinical information were collected. Whole-exome sequencing and sanger sequencing were performed to determine the potential genetic cause of diabetes insipidus. The identified variant was classified according to the American College of Medical Genetics (ACMG) criteria. Bioinformatic analysis was performed to predict the function of the identified variation. Moreover, wild-type and mutated AVPR2 vectors were constructed and transfection to HEK-293T cells. Immunofluorescence experiments were performed to investigate the expression and localization of the mutated protein and cAMP parameter assays were used to measure its activity in response to AVP.

Results: The heights of the adult members affected with polyuria and polydipsia were normal, but all affected children had growth retardation. Next-generation sequencing identified a novel mutation in AVPR2 gene (c.530T > A) in this family. Bioinformatic analysis indicated that the mutation in AVPR2 changed the hydropathic characteristic of the protein and was probably deleterious. Although immunofluorescence showed that the mutated AVPR2 was normally expressed in the cell surface, the intracellular cAMP concentration stimulated by AVP was significantly lower in cells transfected with mutated AVPR2 than cells transfected with wild-type AVPR2. Based on the ACMG criteria, the novel c.530T > A variant of the AVPR2 gene was likely pathogenic and the affected family members were diagnosed as CNDI. After the confirmation of the diagnosis, the proband was treated with compound amiloride hydrochloride and rhGH, the symptoms of polyuria, polydipsia and growth retardation were all improved.

Conclusion: These findings suggested that the novel mutation in AVPR2 (c.530T > A) was a true disease-causing variant with mild effects, which could be classified as a type III mutant receptor. Moreover, investigations of the function of growth hormone axis could be important for the pediatric CNDI patients with extreme short stature, and rhGH treatment might improve the final adult heights in these patients.
Compound Kushen Injection Protects Skin From Radiation Injury via Regulating Bim
Authors: Zheng Jianxiao , Li Gong , Wang Juanjuan , Wang Shujing , Tang Qing , Sheng Honghao , Wu Wanyin , Wang Sumei
Published: December 2021

Background: Radiation-induced skin injury is a major side-effect observed in cancer patients who received radiotherapy. Thus identifying new radioprotective drugs for prevention or treatment of post-irradiation skin injury should be prompted. A large number of clinical studies have confirmed that Compound Kushen injection (CKI) can enhance efficacy and reduce toxicity of radiotherapy. The aim of this study is to confirm the effect of CKI in alleviating radiotherapy injury in the skin and explore the exact mechanism.

Methods: 60 patients who met the inclusion/exclusion criteria were allocated to treatment group (CKI before radiotherapy) or control group (normal saline before radiotherapy) randomly. MTT assay, flow cytometry, Western Blot, and transient transfection were performed to detect the cell viability, cell apoptosis and Bim expression after treatment with CKI or/and radiotherapy.

Results: CKI had the effect of alleviating skin injury in cancer patients who received radiotherapy in clinic. CKI induced cancer cell apoptosis when combined with irradiation (IR), while it reversed the induction of cell apoptosis by IR in human skin fibroblast (HSF) cells. And Bim, as a tumor suppressor, was induced in cancer cells but had no change in HSF cells when treated with CKI. Moreover, the above effect could be attenuated when Bim was silenced by siRNA.

Conclusion: We conclude that CKI represents a promising radio-protective agent with a potential differential beneficial effect on both cancer cells (inducing apoptosis) and HSF cells (providing radio-protection via inhibiting IR-induced apoptosis), via regulating Bim. Our study uncovers a novel mechanism by which CKI inhibits human cancer cell while protects skin from radiotherapy, indicating CKI might be a promising radio-protective drug.

Clinical Trial Registration: Chinese Clinical Trial Registry (www.chictr.org.cn), identifier ChiCTR2100049164.
Case Report: Monitoring Vancomycin Concentrations and Pharmacokinetic Parameters in Continuous Veno-Venous Hemofiltration Patients to Guide Individualized Dosage Regimens: A Case Analysis
Authors: Chen Jihui , Huang Xiaohui , Lin Zhiyan , Li Chao , Ding Haoshu , Du Junming , Li Lixia
Published: December 2021

There are limited pharmacokinetic (PK) studies on vancomycin in patients treated with continuous renal replacement therapy (CRRT), and the results have been inconsistent. Because of individual differences, proposing a definite recommendation for the clinical regimen is not possible. Rapidly reaching target vancomycin concentrations will facilitate effective treatment for critically ill patients treated with CRRT. In this study, to understand the dynamic change in drug clearance rates in vivo, analyze the effect of PK changes on drug concentrations, and recommend loading and maintenance dosage regimens, we monitored the blood concentrations of vancomycin and calculated the area under the curve in two critically ill patients treated with vancomycin and continuous veno-venous hemofiltration (CVVH). On the basis of real-time therapeutic drug monitoring results and PK parameters, an individualized vancomycin regimen was developed for patients with CVVH. Good clinical efficacy was achieved, which provided support and reference for empirical vancomycin therapy in these patients.
Burdock Fructooligosaccharide Attenuates High Glucose-Induced Apoptosis and Oxidative Stress Injury in Renal Tubular Epithelial Cells
Authors: Ding Mengru , Tang Zhiyan , Liu Wei , Shao Taili , Yuan Pingchuan , Chen Kaoshan , Zhou Yuyan , Han Jun , Zhang Jing , Wang Guodong
Published: December 2021

Hyperglycemia-induced apoptosis and oxidative stress injury are thought to play important roles in the pathogenesis of diabetic nephropathy (DN). Attenuating high glucose (HG)-induced renal tubular epithelial cell injury has become a potential approach to ameliorate DN. In recent years, burdock fructooligosaccharide (BFO), a water-soluble inulin-type fructooligosaccharide extracted from burdock root, has been shown to have a wide range of pharmacological activities, including antiviral, anti-inflammatory, and hypolipidemic activities. However, the role and mechanism of BFO in rat renal tubular epithelial cells (NRK-52E cells) have rarely been investigated. The present study investigated the protective effect of BFO on HG-induced damage in NRK-52E cells. BFO could protect NRK-52E cells against the reduced cell viability and significantly increased apoptosis rate induced by HG. These anti-oxidative stress effects of BFO were related to the significant inhibition of the production of reactive oxygen species, stabilization of mitochondrial membrane potential, and increased antioxidant (superoxide dismutase and catalase) activities. Furthermore, BFO increased the expression of Nrf2, HO-1, and Bcl-2 and decreased the expression of Bax. In conclusion, these findings suggest that BFO protects NRK-52E cells against HG-induced damage by inhibiting apoptosis and oxidative stress through the Nrf2/HO-1 signaling pathway.
Closed-Loop Multiscale Computational Model of Human Blood Circulation. Applications to Ballistocardiography
Authors: Rabineau Jeremy , Nonclercq Antoine , Leiner Tim , van de Borne Philippe , Migeotte Pierre-Francois , Haut Benoit
Published: December 2021

Cardiac mechanical activity leads to periodic changes in the distribution of blood throughout the body, which causes micro-oscillations of the body’s center of mass and can be measured by ballistocardiography (BCG). However, many of the BCG findings are based on parameters whose origins are poorly understood. Here, we generate simulated multidimensional BCG signals based on a more exhaustive and accurate computational model of blood circulation than previous attempts. This model consists in a closed loop 0D-1D multiscale representation of the human blood circulation. The 0D elements include the cardiac chambers, cardiac valves, arterioles, capillaries, venules, and veins, while the 1D elements include 55 systemic and 57 pulmonary arteries. The simulated multidimensional BCG signal is computed based on the distribution of blood in the different compartments and their anatomical position given by whole-body magnetic resonance angiography on a healthy young subject. We use this model to analyze the elements affecting the BCG signal on its different axes, allowing a better interpretation of clinical records. We also evaluate the impact of filtering and healthy aging on the BCG signal. The results offer a better view of the physiological meaning of BCG, as compared to previous models considering mainly the contribution of the aorta and focusing on longitudinal acceleration BCG. The shape of experimental BCG signals can be reproduced, and their amplitudes are in the range of experimental records. The contributions of the cardiac chambers and the pulmonary circulation are non-negligible, especially on the lateral and transversal components of the velocity BCG signal. The shapes and amplitudes of the BCG waveforms are changing with age, and we propose a scaling law to estimate the pulse wave velocity based on the time intervals between the peaks of the acceleration BCG signal. We also suggest new formulas to estimate the stroke volume and its changes based on the BCG signal expressed in terms of acceleration and kinetic energy.
The Dynamic Relationship Between Cortical Oxygenation and End-Tidal CO2 Transient Changes Is Impaired in Mild Cognitive Impairment Patients
Authors: Marmarelis Vasilis Z. , Shin Dae C. , Zhang Rong
Published: December 2021

Background: Recent studies have utilized data-based dynamic modeling to establish strong association between dysregulation of cerebral perfusion and Mild Cognitive Impairment (MCI), expressed in terms of impaired CO2 dynamic vasomotor reactivity in the cerebral vasculature. This raises the question of whether this is due to dysregulation of central mechanisms (baroreflex and chemoreflex) or mechanisms of cortical tissue oxygenation (CTO) in MCI patients. We seek to answer this question using data-based input-output predictive dynamic models.

Objective: To use subject-specific data-based multivariate input-output dynamic models to quantify the effects of systemic hemodynamic and blood CO2 changes upon CTO and to examine possible differences in CTO regulation in MCI patients versus age-matched controls, after the dynamic effects of central regulatory mechanisms have been accounted for by using cerebral flow measurements as another input.

Methods: The employed model-based approach utilized the general dynamic modeling methodology of Laguerre expansions of kernels to analyze spontaneous time-series data in order to quantify the dynamic effects upon CTO (an index of relative capillary hemoglobin saturation distribution measured via near-infrared spectroscopy) of contemporaneous changes in end-tidal CO2 (proxy for arterial CO2), arterial blood pressure and cerebral blood flow velocity in the middle cerebral arteries (measured via transcranial Doppler). Model-based indices (physio-markers) were computed for these distinct dynamic relationships.

Results: The obtained model-based indices revealed significant statistical differences of CO2 dynamic vasomotor reactivity in cortical tissue, combined with “perfusivity” that quantifies the dynamic relationship between flow velocity in cerebral arteries and CTO in MCI patients versus age-matched controls (p = 0.006). Significant difference between MCI patients and age-matched controls was also found in the respective model-prediction accuracy (p = 0.0001). Combination of these model-based indices via the Fisher Discriminant achieved even smaller p-value (p = 5 × 10–5) when comparing MCI patients with controls. The differences in dynamics of CTO in MCI patients are in lower frequencies (<0.05 Hz), suggesting impairment in endocrine/metabolic (rather than neural) mechanisms.

Conclusion: The presented model-based approach elucidates the multivariate dynamic connectivity in the regulation of cerebral perfusion and yields model-based indices that may serve as physio-markers of possible dysregulation of CTO during transient CO2 changes in MCI patients.
User Evaluation of Passenger Assistance System Concepts on Public Highways
Authors: Ittner Sandra , Mühlbacher Dominik , Neukum Alexandra , Weisswange Thomas H.
Published: December 2021

There is ample research on assistance systems for drivers in conventional and automated vehicles. In the past, those systems were developed to increase safety but also to increase driver comfort. Since many common risks have by now been mitigated through such systems, the research and development focus expanded to also include comfort-related assistance. However, the passenger has rarely been taken into account explicitly, although it has been shown that passenger discomfort is a relevant problem. Therefore, this work investigated the potential of passenger assistance systems to reduce such discomfort. Three different passenger assistant system prototypes were tested in a driving study on public highway with N = 19 participants. The systems provided information about parameters related to the performance of the driver and one additionally provided a communicative means of influence. For two passenger assistant systems, it could be shown that they significantly reduced passenger discomfort in at least a subset of the evaluated situations. The majority of participants rated one or multiple of the assistant systems as more comfortable than a ride without assistance. The system providing information about the attentiveness of the driver was most effective in reducing discomfort and was rated as the most helpful system. The results show that explicitly considering the situation of passengers in the design of assistance systems can positively impact their comfort. This can be achieved using information from common systems targeting driver assistance available to the passenger.
Higher Residence Attachment and Religiosity Are Associated With Less Depressive Symptoms After Terror Event Exposure
Authors: Korn Liat , Billig Miriam , Zukerman Gil
Published: December 2021

Introduction: We examined how community type, residence attachment, and religiosity contribute to resilience to depressive symptoms, psychosomatic complaints, residential stress, and avoidance behavior among students exposed to terror.

Methods: Undergraduate students from Ariel University (N = 1,413; 62.7% females; Mage = 26.5; SD = 6.03) completed a self-report questionnaire on socio-demographics, terror exposure, place attachment, and depressive/psychosomatic symptoms. Participants were divided into three residential groups: “Ariel,” “Small settlement communities in Judea and Samaria” or “Other places in Israel.”

Results: Participants from small settlement communities in Judea and Samaria showed significantly fewer depressive symptoms and greater adjustment– less avoidance, psychosomatic symptoms, and residential stress– compared to those living in Ariel or other places in Israel, despite significantly higher exposure to terror.

Conclusion: Greater religiosity and residence attachment may protect against depressive symptom development following terror exposure. Secular, temporary residents living in highly terror-exposed areas should be targeted for community strengthening interventions.
The Consideration of Future Consequences Scale Among Malaysian Young Adults: A Psychometric Evaluation
Authors: She Long , Ma Lan , Khoshnavay Fomani Fatemeh
Published: December 2021

Background: The consideration of future consequences (CFC) determines the extent to which individuals consider the potential future outcomes of their current behavior. The significance of assessing the CFC scale’s validation in different contexts has been acknowledged by the previous studies. While the majority of the studies have been conducted in western countries, no study has been conducted in Malaysia. The aim of the current study was to validate a Malaysian version of the CFC scale among Malaysian young adults.

Methods: The methodological cross-sectional approach was adopted in this study. The study recruited 529 young adults (age range from 25 to 40) who fulfilled the inclusion criteria of the paper survey. Construct validity was assessed using content validity, convergent validity, and discriminant validity. Cronbach’s alpha, McDonald’s omega, and average inter-item correlation (AIC) were used to assess the scale’s internal consistency. Also, composite reliability (CR) and maximal reliability (MaxR) were used to assess the construct reliability. Measurement invariance was tested across gender.

Results: The findings of the exploratory factor analysis indicated that the Malaysian version of the CFC scale has a two-factor structure (i.e., CFC-Future and CFC-Immediate) with 10-item explaining 61.682% of the total variance. The confirmatory factor analysis (CFA) supported the two-factor structure of the CFC scale with good construct validity. The internal consistency and CR were acceptable. [The Cronbach’s alpha, McDonald’s omega, and CR for CFC-I were 0.901 (CI 95%: 0.881–918), 0.901, and 0.887, respectively. Also, these parameters for CFC-F were 0.867 (CI 95%: 0.838–891), 0.868, and 0.867, respectively].

Conclusion: We found acceptable psychometric evidence for the 10-item two-factors CFC scale used in the context of young adults in Malaysia. The validated instrument can be used in future studies to assess young adults’ CFC tendency and CFC-related behavior in Malaysia.
Case Report: Repeated Transcranial Magnetic Stimulation Improves Comorbid Binge Eating Disorder in Two Female Patients With Treatment-Resistant Bipolar Depression
Authors: Sciortino Domenico , Schiena Giandomenico , Cantù Filippo , Maggioni Eleonora , Brambilla Paolo
Published: December 2021

Introduction: Binge eating disorder (BED) is the most common eating disorder, affecting a large population worldwide. It is characterized by recurrent episodes of binge eating, with no compensatory behaviors. BED is often associated with psychiatric comorbidities, and still represents a challenge in terms of treatment strategies. In the last years, neuromodulation has represented a promising approach in the treatment of BED. We report the cases of two women, affected by Bipolar Disorder Type II (BD-II) and comorbid BED, whose BED symptoms improved after a course of accelerated intermittent Theta Burst Stimulation (iTBS).

Methods: We carried out a clinical study, involving neurostimulation on six patients with a treatment-resistant depressive episode. The trial consisted of a 3-week accelerated iTBS treatment, delivered to the left dorsolateral pre-frontal cortex. Clinical evaluation scales (Hamilton Rating Scale for Depression, Montgomery-Åsberg Depression Rating Scale, and Young Mania Rating Scale) were administered at baseline, after 2 weeks, and at the end of the stimulation cycle. Pharmacotherapy was maintained unchanged during iTBS treatment. Patients gave their informed consent both for the protocol and for the publication.

Results: The treatment was well-tolerated. Depressive symptoms only slightly improved; however, patients' binge episodes remitted completely, which was a serendipitous finding. BED symptomatology complete remission lasted up to 12 weeks follow-up.

Discussion: This is the first study regarding iTBS use in BED in comorbidity with BD-II. Further research is still needed to assess the efficacy of this technique in BED treatment.
Persuasive Apps for Sustainable Waste Management: A Comparative Systematic Evaluation of Behavior Change Strategies and State-of-the-Art
Authors: Nkwo Makuochi , Suruliraj Banuchitra , Orji Rita
Published: December 2021

With the proliferation of ubiquitous computing and mobile technologies, mobile apps are tailored to support users to perform target behaviors in various domains, including a sustainable future. This article provides a systematic evaluation of mobile apps for sustainable waste management to deconstruct and compare the persuasive strategies employed and their implementations. Specifically, it targeted apps that support various sustainable waste management activities such as personal tracking, recycling, conference management, data collection, food waste management, do-it-yourself (DIY) projects, games, etc. The authors who are persuasive technology researchers retrieved a total of 244 apps from App Store and Google Play, out of which 148 apps were evaluated. Two researchers independently analyzed and coded the apps and a third researcher was involved to resolve any disagreement. They coded the apps based on the persuasive strategies of the persuasive system design framework. Overall, the findings uncover that out of the 148 sustainable waste management apps evaluated, primary task support was the most employed category by 89% (n = 131) apps, followed by system credibility support implemented by 76% (n = 112) apps. The dialogue support was implemented by 71% (n = 105) apps and social support was the least utilized strategy by 34% (n = 51) apps. Specifically, Reduction (n = 97), personalization (n = 90), real-world feel (n = 83), surface credibility (n = 83), reminder (n = 73), and self-monitoring (n = 50) were the most commonly employed persuasive strategies. The findings established that there is a significant association between the number of persuasive strategies employed and the apps’ effectiveness as indicated by user ratings of the apps. How the apps are implemented differs depending on the kind of sustainable waste management activities it was developed for. Based on the findings, this paper offers design implications for personalizing sustainable waste management apps to improve their persuasiveness and effectiveness.
Socio-Ecological Conflicts in a Global South Metropolis: Opportunities and Threats of a Potential Greenway in the São Paulo Metropolitan Region
Authors: Moreno Robson da Silva , Braga Diego Rafael Galvão Cesar , Xavier Luis Felipe
Published: December 2021

Greenways are a measure of environmental remediation within a broad framework aimed at promoting urban greening and adaptation to climate change. The typical characteristics of large urban agglomerations, including land use (such as commercial, industrial, and residential areas) with few public spaces and fragmented landscapes, make it difficult to apply these solutions to the urban fabric, forcing decision-makers and planners to act in informal settlements, highways, and industrial parks. One proposed area is an enclave with unused or underutilized lots, where fragments of the Atlantic Forest, parks, landfills, and rapidly expanding informal settlements can be found. This manuscript examines the socioeconomic and environmental processes that shaped this potential urban greenway between Santo André, Mauá, and Ribeirão Pires, which are part of the São Paulo Metropolitan Region (SPRM), the largest in South America. A survey was conducted based on municipal and regional plans, the environmental and urban laws of Brazil, and the socioeconomic history of this part of the SPRM. In addition, satellite images were used to analyze land use evolution through geotechnologies. Finally, we prepared land use recommendations, considering opportunities and threats, highlighting the possibilities of protection and expansion of the Atlantic Forest. To this end, we examined the literature on environmental urban planning and design, green infrastructure, and other concepts. This study intends to stimulate researchers, planners, and decision-makers regarding the urban greening process in the Global South. According to the recommendations, this stimulus would develop these concepts according to the real situation of the region, which would combine the protection of wild habitats and urban environmental amenities. However, this effort makes no sense if one of the defining Global South characteristics not addressed is social inequality. Therefore, we recommend that an effort be made to develop and incorporate processes from urban greening in slum upgrading.
Optic Foraminotomy for Clipping of Superior Carotid-Ophthalmic Aneurysms
Authors: Baldoncini Matias , Luzzi Sabino , Giotta Lucifero Alice , Flores-Justa Ana , González-López Pablo , Campero Alvaro , Villalonga Juan F. , Lawton Michael T.
Published: December 2021

Background: Carotid-ophthalmic aneurysms usually cause visual problems. Its surgical treatment is challenging because of its anatomically close relations to the optic nerve, carotid artery, ophthalmic artery, anterior clinoid process, and cavernous sinus, which hinder direct access. Despite recent technical advancements enabling risk reduction of this complication, postoperative deterioration of visual function remains a significant problem. Therefore, the goal of preserving and/or improving the visual outcome persists as a paramount concern.

Objective: We propose optic foraminotomy as an alternative microsurgical technique for dorsal carotid-ophthalmic aneurysms clipping. As a secondary objective, the step by step of that technique and its benefits are compared to the current approach of anterior clinoidectomy.

Methods: We present as an example two patients with superior carotid-ophthalmic aneurysms in which the standard pterional craniotomy, transsylvian approach, and optic foraminotomy were performed. Surgical techniques are presented and discussed in detail with the use of skull base dissections, microsurgical images, and original drawings.

Results: Extensive opening of the optic canal and optic nerve sheath was successfully achieved in all patients allowing a working angle with the carotid artery for correct visualization of the aneurysm and further clipping. Significant visual acuity improvement occurred in both patients because of decompression of the optic nerve.

Conclusion: Optic foraminotomy is an easy and recommended technique for exposing and treating superior carotid-ophthalmic aneurysms and allowing optic nerve decompression during the first stages of the procedure. It shows several advantages over the current anterior clinoidectomy technique regarding surgical exposure and facilitating visual improvement.
Feszes íny szélesítés implantátumok körül – „H” technika esetismertetés
Authors: Párkányi László , Fráter Márk
Published: December 2021

Míg a természetes fogak körül a gingiva vastagságának és a feszes íny jelenlétének inkább csak esztétikai jelentősége van, addig a koronai restaurátummal ellátott implantátumok körül a lágyszöveti viszonyok nem csak az implantációs pótlások esztétikai megjelenését, hanem akár az implantátumok hosszú távú sikerességét is befolyásolhatják. Implantátumok mellett hagyományosan a biotípus-módosítás, illetve keratinizált szövetszélesítés külön sebészi beavatkozás eredménye. Ez megtörténhet az implantátum felszabadítása előtt vagy azt követően. A beavatkozáshoz alkalmazott graft rendszerint szabad íny graft (Free Gingival Graft, FGG), mely jelentős donor morbiditást jelenthet, valamint az esztétikájában, megjelenésében eltér a környező lágyszövetekétől. Jelen technika célja a biotípus módosítása és keratinizált szövet szélesítésének elvégzése egyazon lépésben az implantációval, kötőszövet-helyettesítő kollagén mátrix (Acellular dermal matrix, ADM) alkalmazásával, nyitott gyógyulás során. Ezzel egyszerre csökkenthető a donor morbiditás, a sebészi beavatkozások száma (ezáltal a gyógyulási idő), valamint javítható az esztétikai végeredmény.
Orális lichen planus előfordulása diabeteses betegek körében – irodalmi áttekintés
Authors: Szigeti Virág , Németh Zsolt , Ujpál Márta
Published: December 2021

Manapság egyre több olyan irodalmi adatot közölnek, melyek azt igazolják, hogy a cukorbetegek körében gyakrabban fordulnak elő szájüregi praecancerosus elváltozások, elsősorban leukoplákia és lichen. Cikkünkben azokat a PubMeden 1988–2017 között megjelent publikációkat gyűjtöttük össze, amelyek az orális lichen és a diabetes mellitus kapcsolatával foglalkoznak. A lichen planus előfordulása cukorbetegek között ezek alapján 2,52%, addig a kontrollcsoportban 0,97%,a lichen planussal rendelkező betegek között a diabetes gyakorisága pedig 23,32%. A vizsgálatok alapján megállapítható, hogy a lichen planus mint az egyik leggyakoribb rákmegelőző elváltozás, szoros kapcsolatban áll a diabetessel, ezért az érintett betegcsoport szájüregi és metabolikus kontrollja kiemelt figyelmet és interdiszciplináris együttműködést igényel.
Minimál invazív bemeneti kavitás premoláris és moláris fogaknál – irodalmi összefoglalás
Authors: Balázs Mónika , Komora Péter , Tóth Zsuzsanna
Published: December 2021

A közösségi médiában és a tudományos fórumokon is egyre többször jelennek meg esetbemutatások, melyekben miniatűrbemeneti kavitás(ok)on keresztül végeznek gyökérkezelést a vállalkozó szellemű fogorvosok. Az egyértelmű technikaibravúrhoz elengedhetetlen nagyítás (operációs mikroszkóp használata), megfelelő megvilágítás és műszerezettség.Minimál invazív bemeneti kavitás kialakításával a gyökérkezelés kivitelezése nehezebb és időigényesebb lehet, ezértösszetettebb anatómiájú fogaknál csak korlátozottan alkalmazható. Egyszerűbb esetekben azonban, ha a szövettörmelékeltávolítása és a pulpaűr fertőtlenítése biztosított, valós alternatívája lehet a hagyományos bemeneti kavitásoknak.Több koronai foganyag megőrzését teszi lehetővé, így a fog restaurálható marad, de a törési ellenállás növekedését invitro egyelőre nem sikerült igazolni.
Strategies to Increase the Value of Pomaces with Fermentation
Authors: Munekata Paulo E. S. , Domínguez Rubén , Pateiro Mirian , Nawaz Asad , Hano Christophe , Walayat Noman , Lorenzo José M.
Published: December 2021

The generation of pomaces from juice and olive oil industries is a major environmental issue. This review aims to provide an overview of the strategies to increase the value of pomaces by fermentation/biotransformation and explore the different aspects reported in scientific studies. Fermentation is an interesting solution to improve the value of pomaces (especially from grape, apple, and olive) and produce high-added value compounds. In terms of animal production, a shift in the fermentation process during silage production seems to happen (favoring ethanol production rather than lactic acid), but it can be controlled with starter cultures. The subsequent use of silage with pomace in animal production slightly reduces growth performance but improves animal health status. One of the potential applications in the industrial context is the production of enzymes (current challenges involve purification and scaling up the process) and organic acids. Other emerging applications are the production of odor-active compounds to improve the aroma of foods as well as the release of bound polyphenols and the synthesis of bioactive compounds for functional food production.
Microbiological Status of Venous Leg Ulcers and Its Predictors: A Single-Center Cross-Sectional Study
Authors: Cwajda-Białasik Justyna , Mościcka Paulina , Jawień Arkadiusz , Szewczyk Maria Teresa
Published: December 2021

Venous leg ulcers are frequently colonized by microbes. This can be particularly devastating if the ulcer is infected with alert pathogens, i.e., highly virulent microorganisms with well-developed mechanisms of antibiotic resistance. We analyzed the microbiological status of venous leg ulcers and identified the clinicodemographic predictors of culture-positive ulcers, especially in ulcers with colonization by alert pathogens. Methods: This study included 754 patients with chronic venous leg ulcers. Material for microbiological analysis was collected by swabbing only from patients who did not receive any antibiotic treatment. Results: A total of 636 (84.3%) patients presented with culture-positive ulcers. Alert pathogens, primarily Pseudomonas aeruginosa, were detected in 28.6% of the positive cultures. In a logistic regression model, culture-positive ulcers were predicted independently by age > 65 years, current ulcer duration > 12 months, and ulceration area greater than 8.25 cm2. Two of these factors, duration of current ulcer > 12 months and ulceration area > 8.25 cm2, were also identified as the independent predictors of colonization by alert pathogens. Conclusions: Colonization/infection is particularly likely in older persons with chronic and/or large ulcers. Concomitant atherosclerosis was an independent predictor of culture-negative ulcers.
Synthesis, Structural Characterization, and In Vitro and In Silico Antifungal Evaluation of Azo-Azomethine Pyrazoles (PhN2(PhOH)CHN(C3N2(CH3)3)PhR, R = H or NO2)
Authors: Fernandez Dorancelly , Restrepo-Acevedo Andrés , Rocha-Roa Cristian , Le Lagadec Ronan , Abonia Rodrigo , Zacchino Susana A. , Gómez Castaño Jovanny A. , Cuenú-Cabezas Fernando
Published: December 2021

The azo-azomethine imines, R1-N=N-R2-CH=N-R3, are a class of active pharmacological ligands that have been prominent antifungal, antibacterial, and antitumor agents. In this study, four new azo-azomethines, R1 = Ph, R2 = phenol, and R3 = pyrazol-Ph-R’ (R = H or NO2), have been synthesized, structurally characterized using X-ray, IR, NMR and UV–Vis techniques, and their antifungal activity evaluated against certified strains of Candida albicans and Cryptococcus neoformans. The antifungal tests revealed a high to moderate inhibitory activity towards both strains, which is regulated as a function of both the presence and the location of the nitro group in the aromatic ring of the series. These biological assays were further complemented with molecular docking studies against three different molecular targets from each fungus strain. Molecular dynamics simulations and binding free energy calculations were performed on the two best molecular docking results for each fungus strain. Better affinity for active sites for nitro compounds at the “meta” and “para” positions was found, making them promising building blocks for the development of new Schiff bases with high antifungal activity.
Preparation and Characterization of Indomethacin Supramolecular Systems with β-Cyclodextrin in Order to Estimate Photostability Improvement
Authors: Jamrógiewicz Marzena , Józefowicz Marek
Published: December 2021

Cyclodextrins have found wide application in contemporary chemistry, pharmacy and medicine. Because of their unique properties, cyclodextrins are constantly used in research on solubility or stability improvement, as well as other physicochemical properties of medicinal substances. Indomethacin (IND) is a photolabile molecule that also attracts the interest of researchers due to its therapeutic potential and the need to overcome its problematic photosensitivity. Supramolecular complexes of indomethacin with β-cyclodextrin (CD) are already known, and they show greater stability compared to complexes with other types of cyclodextrins. So far, however, the sensitivity to light of physical mixtures and inclusion complexes in the solid phase has not been studied, and their various stoichiometries have not yet been investigated. Due to this fact, the aim of the present study is to obtain supramolecular systems (inclusion complexes and physical mixtures) of indomethacin with three different amounts of β-cyclodextrin. Assessment of the photochemical stability of indomethacin-β-cyclodextrin systems in the solid state is performed in order to find the best correlation between IND stability and the amount of CD. Comparative analysis of physicochemical degradation for stoichiometry systems [CD:IND] = [1:1], [0.5:1] and [0.1:1] is performed by using ultraviolet spectroscopy, transmission—FTIR, reflection—ATR-FTIR infrared spectroscopy and DSC calorimetry.
Comparative Analysis of Major Flavonoids among Parts of Lactuca indica during Different Growth Periods
Authors: Hao Junfeng , Li Yuyu , Jia Yushan , Wang Zhijun , Rong Rong , Bao Jian , Zhao Muqier , Fu Zhihui , Ge Gentu
Published: December 2021

L. indica L. cv. Mengzao, a medicinal plant of the Ixeris genus, is rich in flavonoids. In order to thoroughly analyze the the distribution and dynamic change of major flavonoids in its various parts from different growth periods, the flavonoids extracted from L. indica L. cv. Mengzao were identified and quantitatively analyzed by ultra-high-performance liquid chromatography mass spectrometer (LC-MS/MS). Results indicated that 15 flavonoids were identified from L. indica L. cv. Mengzao, and rutin, luteolin, luteolin-7-O-glucoside, kaempferol, quercetin, and apigenin are the major flavonoids in L. indica L. cv. Mengzao. In general, the total flavonoids’ content in different parts of L. indica L. cv. Mengzao followed the order flowers > leaves > stems > roots. Flowers and leaves are the main harvesting parts of L. indica L. cv. Mengzao, and the flowering period is the most suitable harvesting period. This study provides valuable information for the development and utilization of L. indica L. cv. Mengzao and determined the best part to harvest and the optimal time for harvesting.
Cognitively Diagnostic Analysis Using the G-DINA Model in R
Authors: Shi Qingzhou , Ma Wenchao , Robitzsch Alexander , Sorrel Miguel A. , Man Kaiwen
Published: December 2021

Cognitive diagnosis models (CDMs) have increasingly been applied in education and other fields. This article provides an overview of a widely used CDM, namely, the G-DINA model, and demonstrates a hands-on example of using multiple R packages for a series of CDM analyses. This overview involves a step-by-step illustration and explanation of performing Q-matrix evaluation, CDM calibration, model fit evaluation, item diagnosticity investigation, classification reliability examination, and the result presentation and visualization. Some limitations of conducting CDM analysis in R are also discussed.
Bench Testing of Sensors Utilized for In-Line Monitoring of Lubricants and Hydraulic Fluids Properties
Authors: Pochi Daniele , Grilli Renato , Fornaciari Laura , Betto Monica , Benigni Stefano , Fanigliulo Roberto
Published: December 2021

This work reports the results of a study on the behaviour of five sensors recently developed for oil conditions monitoring, installed in-line in an experimental test rig for lubricants. The tests were carried out on seven oils of different origins (one synthetic ester, two bio-based synthetic esters, four vegetable oils) and use (two UTTOs and five hydraulic oils), under controlled working conditions, according to a specially designed test method. At first, the study concerned the identification of the conditions for the correct sensors’ installation. Then, the tests started applying to the fluids severe work cycles intended to accelerate oil ageing. The data of viscosity, permittivity, relative humidity, electric conductivity, particle contamination, and ferro-magnetic particles provided by the sensors were compared to the results of laboratory analyses made on oil samples taken during the tests with the aim of verifying the sensors measurements accuracy and reliability and selecting the more suitable ones to in-line oil conditions monitoring, in the perspective of introducing them also in field applications, e.g., on agricultural tractors, for preventing damages due to oil deterioration, and in managing the machine maintenance.