bluuebunny/crossref_metadata_2025 huggingface dataset:
Shear-induced incipient motion of a single sphere on uniform substrates at low particle Reynolds numbers
Authors: Agudo J. R. , Illigmann C. , Luzi G. , Laukart A. , Delgado A. , Wierschem A.
Published: July 2017

We study the incipient motion of single spheres in steady shear flow on regular substrates at low particle Reynolds numbers. The substrate consists of a monolayer of regularly arranged fixed beads, in which the spacing between beads is varied resulting in different angles of repose and exposures of the particle to the main flow. The flow-induced forces and the level of flow penetration into the substrate are determined numerically. Since experiments in this range had shown that the critical Shields number is independent of inertia but strongly dependent on the substrate geometry, the particle Reynolds number was fixed to 0.01 in the numerical study. Numerics indicates that rolling motion is always preferred to sliding and that the flow penetration is linearly dependent on the spacing between the substrate particles. Besides, we propose an analytical model for the incipient motion. The model is an extension of Goldman’s classical result for a single sphere near a plain surface taking into account the angle of repose, flow orientation with respect to substrate topography and shielding of the sphere to the linear shear flow. The effective level of flow penetration is the only external parameter. The model, applied to triangular and quadratic arrangements with different spacings, is able to predict the dependence of the critical Shields number on the geometry and on the orientation of the substrate. The model is in very good agreement with numerical results. For well-exposed particles, we observed that the minimum critical Shields number for a certain angle of repose does not depend sensitively on the considered arrangement. At large angles of repose, as expected in fully armoured beds, the model is consistent with experimental results for erodible beds at saturated conditions.
Autonomous propulsion of nanorods trapped in an acoustic field
Authors: Collis Jesse F. , Chakraborty Debadi , Sader John E.
Published: July 2017

Acoustic fields in a liquid medium can trap and suspend small particles at their pressure nodes. Recent measurements demonstrate that nanorods immersed in these fields generate autonomous propulsion, with their direction and speed controlled by both the particle’s shape and density distribution. Specifically, slender nanorods with an asymmetric density distribution about their geometric centre are observed to move steadily with their low density end leading the motion; particle geometry exerts an equally significant and potentially opposing effect. In this article, we investigate the physical mechanisms underlying this combined density/shape induced phenomenon by developing a simple yet rigorous mathematical framework for axisymmetric particles. This only requires solution of the (linear) unsteady Stokes equations, which can be performed numerically or analytically. The theory holds for all particle shapes, particle aspect ratios (length/width) and acoustic frequencies. It is applied to slender dumbbell-shaped particles and asymmetric nanorods – these provide model systems to investigate the competing effects governing propulsion. This shows that geometric and density asymmetries in the particle generate axial jets that can produce motion in either direction, depending on the relative strengths of these asymmetries and the acoustic Reynolds number (dimensionless frequency). Strikingly, the propulsion direction is found to reverse with increasing frequency, an effect that is yet to be reported experimentally. The general theory and mechanism described here enable thea prioridesign and fabrication of nano-motors in fluid for transport of small-scale payloads and robotic applications.
Dynamo action in rapidly rotating Rayleigh–Bénard convection at infinite Prandtl number
Authors: Cattaneo Fausto , Hughes David W.
Published: July 2017

In order better to understand the processes that lead to the generation of magnetic fields of finite amplitude, we study dynamo action driven by turbulent Boussinesq convection in a rapidly rotating system. In the limit of infinite Prandtl number (the ratio of viscous to thermal diffusion) the inertia term drops out of the momentum equation, which becomes linear in the velocity. This simplification allows a decomposition of the velocity into a thermal part driven by buoyancy, and a magnetic part driven by the Lorentz force. While the former velocity defines the kinematic dynamo problem responsible for the exponential growth of the magnetic field, the latter encodes the magnetic back reaction that leads to the eventual nonlinear saturation of the dynamo. We argue that two different types of solution should exist: weak solutions in which the saturated velocity remains close to the kinematic one, and strong solutions in which magnetic forces drive the system into a new strongly magnetised state that is radically different from the kinematic one. Indeed, we find both types of solutions numerically. Interestingly, we also find that, in our inertialess system, both types of solutions exist on the same subcritical branch of solutions bifurcating from the non-magnetic convective state, in contrast with the more traditional situation for systems with finite inertia in which weak and strong solutions are thought to exist on different branches. We find that for weak solutions, the force balance is the same as in the non-magnetic case, with the horizontal size of the convection varying as the one-third power of the Ekman number (the ratio of viscous to Coriolis forces), which gives rise to very small cells at small Ekman numbers (i.e. high rotation rates). In the strong solutions, magnetic forces become important and the convection develops on much larger horizontal scales. However, we note that even in the strong cases the solutions never properly satisfy Taylor’s constraint, and that viscous stresses continue to play a role. Finally, we discuss the relevance of our findings to the study of planetary dynamos in rapidly rotating systems such as the Earth.
The turbulence cascade in the near wake of a square prism
Authors: Alves Portela F. , Papadakis G. , Vassilicos J. C.
Published: July 2017

We present a study of the turbulence cascade on the centreline of an inhomogeneous and anisotropic near-field turbulent wake generated by a square prism at a Reynolds number of$Re=3900$using the Kármán–Howarth–Monin–Hill equation. This is the fully generalised scale-by-scale energy balance which, unlike the Kármán–Howarth equation, does not require homogeneity or isotropy assumptions. Our data are obtained from a direct numerical simulation and therefore enable us to access all of the processes involved in this energy balance. A significant range of length scales exists where the orientation-averaged nonlinear interscale transfer rate is approximately constant and negative, indicating a forward turbulence cascade on average. This average cascade consists of coexisting forward and inverse cascade behaviours in different scale-space orientations. With increasing distance from the prism but within the near field of the wake, the orientation-averaged nonlinear interscale transfer rate tends to be approximately equal to minus the turbulence dissipation rate even though all of the inhomogeneity-related energy processes in the scale-by-scale energy balance are significant, if not equally important. We also find well-defined near$-5/3$energy spectra in the streamwise direction, in particular at a centreline position where the inverse cascade behaviour occurs for streamwise oriented length scales.
Direct numerical simulations of two-phase flow in an inclined pipe
Authors: Xie Fangfang , Zheng Xiaoning , Triantafyllou Michael S. , Constantinides Yiannis , Zheng Yao , Em Karniadakis George
Published: July 2017

We study the instability mechanisms leading to slug flow formation in an inclined pipe subject to gravity forces. We use a phase-field approach, where the Cahn–Hillard model is used to model the interface. At the inlet, a stratified flow is imposed with a specified velocity profile. We validate our numerical results by comparing against previous theoretical models and by predicting the various flow regimes for horizontal and inclined pipes, including stratified flow, slug flow, dispersed bubble flow and annular flow. Subsequently, we focus on slug formation in an inclined pipe and connect its appearance with specific vortical dynamics. A two-dimensional channel geometry is first considered. When the heavy fluid is injected as the top layer, inverted vortex shedding emerges, which periodically impacts on the bottom wall, as it develops further downstream. The accumulation of heavy fluid in the bottom wall causes a back flow that induces rolling waves and interacts with the upstream jet. When the heavy fluid is placed as the bottom layer, the heavy fluid accumulates and initially forms a massive slug at the bottom region, close to the inlet. Subsequently, the heavy fluid slug starts to break into smaller pieces, some of which translate along the pipe. During the accumulation phase, a back flow forms also generating rolling waves. Occasionally, a rolling wave can reach the top of the pipe and form a new slug. To describe the generation of vorticity from the two-phase interface and pipe walls in the slug formation, we study the circulation dynamics and connect it with the resulting two-phase flow patterns. Finally, we conduct three-dimensional (3-D) simulations in a circular pipe and compare the differences between the 3-D flow patterns and its circulation dynamics against the 2-D simulation results.
An Implicit Stereotype of the Rich and Its Relation to Psychological Connectedness
Authors: Liu Chang-Jiang , Zhang Yue , Hao Fang
Published: July 2017

This study investigates people's implicit stereotype of the social group of the rich in terms of competence and warmth. We further examine the stereotype's relationship with temporal selves. Implicit Association Tests were used as measures of implicit social perception in a social comparison context. We also rated the degree of psychological connectedness between current and possible future selves across time. Our results demonstrate that the rich are implicitly perceived as having high levels of competence and low levels of warmth compared to the average person, and that a close psychological connectedness mitigates the negative perception of the rich. The implications and limitations of these findings are also discussed.
MANGANESE NUTRITION IMPROVES THE PRODUCTIVITY AND GRAIN BIOFORTIFICATION OF BREAD WHEAT IN ALKALINE CALCAREOUS SOIL
Authors: ULLAH AMAN , FAROOQ MUHAMMAD , REHMAN ABDUL , ARSHAD MUHAMMAD SHAKEEL , SHOUKAT HIRA , NADEEM ASIF , NAWAZ AHMAD , WAKEEL ABDUL , NADEEM FAISAL
Published: July 2017

Manganese (Mn) is one important microelement for plants and the human beings. This study was conducted to evaluate the potential of Mn nutrition in improving the productivity and grain biofortification of wheat. For optimization of Mn seed treatments, seeds were primed with 0.1 and 0.01 M Mn solution, or were coated with 250 and 500 mg Mn kg−1 seed. The optimized treatments were used in the second experiment replicated over time and space. In the first experiment conducted at Faisalabad during 2012–2013, maximum grain yield was recorded with Mn seed priming (0.1 M Mn solution), while maximum grain Mn concentration was recorded with foliar application of 0.75 M Mn solution and seed coating with 250 mg Mn kg−1 seed. In the second experiment, conducted at Faisalabad and Sheikhupura during 2013–2014, and at Faisalabad during 2014–2015, maximum grain yield and grain Mn concentration were recorded from seed priming with 0.1 M Mn solution. Regardless of method, Mn application improved the productivity and grain biofortification of wheat. Overall order of improvement in grain yield was seed priming (3.87 Mg ha−1) > foliar application (3.74 Mg ha−1) > seed coating (3.57 Mg ha−1). Regarding grain Mn concentration, the best treatment was seed priming (41.40 µg g−1) followed by seed coating (39.87 µg g−1) and foliar application (36.94 µg g−1). Maximum net returns and benefit-cost ratio were obtained with Mn seed priming, while maximum marginal rate of return was obtained with Mn seed coating. In conclusion, Mn application through seed treatments was cost effective for improving the productivity and grain biofortification of bread wheat in alkaline calcareous soil.
Pandemic-related health behavior: repeat episodes of influenza-like illness related to the 2009 H1N1 influenza pandemic
Authors: MGBERE O. , NGO K. , KHUWAJA S. , MOUZOON M. , GREISINGER A. , ARAFAT R. , MARKEE J.
Published: July 2017

The Houston Health Department (HHD) in Texas tracks influenza-like illness (ILI) in the community through its Influenza Sentinel Surveillance Program, which began in 2008. After the influenza A(H1N1) pandemic (pH1N1) in 2009, investigators sought to assess the feasibility of this program as a non-traditional data source for tracking and monitoring care-seeking activities. Through the process of characterizing and describing patients who had ‘return visits’, or who were considered the heaviest ILI-related care-utilizers, the investigators sought to understand the strengths and limitations of this data source. Data used for this study were obtained from a multispecialty clinic in Houston, Texas between August 2008 and January 2011 across three phases: pre-pH1N1, pH1N1, and post-pH1N1. The data, which comprised of 4047 patient visits, yielded 150 return visits. We found an increase in the number of visits for ILI and proportion of return visits during the pandemic phase (pH1N1), as well as differences in the likelihood of a return visit between genders and age groups. More broadly, the findings of this study provide important considerations for future research and expose important gaps in using surveillance data to assess sick-role behaviors.
OVERCOMING FINANCIAL FRICTIONS WITH THE FRIEDMAN RULE
Authors: Salas Sergio
Published: July 2017

A general equilibrium model with financial frictions in which individuals may encounter unobservable investment opportunities is developed along the lines of Kiyotaki and Moore (2012). I study efficiency properties induced by money and monetary policy when financial frictions prevent optimal equilibrium allocations. By providing closed-form solutions to all prices, allocations, welfare, and, especially, the distribution of individuals with respect to assets, I show that the Friedman rule achieves maximal social welfare, independent of how tight the financial constraints may be. The same level of welfare would be induced by an omniscient central planner able to verify who has an investment opportunity.
BUSINESS UNCERTAINTY AND THE EFFECTIVENESS OF FISCAL POLICY IN GERMANY
Authors: Berg Tim Oliver
Published: July 2017

There are suggestions that increased uncertainty makes fiscal policy temporarily less effective. In this paper, I examine the relationship between business uncertainty and fiscal policy effectiveness in Germany. I use measures of business uncertainty that are derived from the firm-level data of the Ifo Business Climate Survey and interact them with the parameters of a structural vector autoregression to produce state-dependent spending multipliers. The impact of increased uncertainty on the spending multiplier is generally small and often statistically not significant in the short run. By contrast, I obtain a significant positive impact on the long-run multiplier. These baseline results are supported by a variety of robustness checks and specifications.
A new set of microsatellite loci for Cattleya walkeriana Gardner, an endangered tropical orchid species and its transferability to Cattleya loddigesii Lindl. and Cattleya nobilior Reichenbach
Authors: Rossini Bruno C. , Kampa Mariá B. , Marino Celso L. , Pereira Fernanda Bortolanza , Alves Fábio M. , Pereira de Souza Anete , Ibanes Bruna , Schwarcz Kaiser Dias , Zucchi Maria Imaculada , Bobrowski Rogério , Peres Fabiana S. B. , Tambarussi Evandro Vagner
Published: July 2017

In this study, we isolate and analyse a new set of microsatellite loci for Cattleya walkeriana. Twenty-two primer pairs were screened for C. walkeriana (n = 32) and assessed for their transferability to Cattleya loddigesii (n = 12) and Cattleya nobilior (n = 06). All loci amplified for C. walkeriana; however, for C. loddigesii and C. nobilior, four and five primers, respectively, did not present amplification. The polymorphic loci presented between 2 and 13 alleles per locus for both C. walkeriana and C. loddigesii, with respective averages of 5.1 and 4.2. For C. nobilior, we found between two and five alleles per locus, with an average of 2.6. For C. walkeriana, observed heterozygosity varied from 0.100 to 0.966, whereas expected heterozygosity ranged from 0.097 to 0.900. The observed and expected heterozygosity for C. loddigesii and C. nobilior were also estimated. We found no significant linkage disequilibrium between any pair of loci, and evidence of null alleles at four loci (Cw16, Cw24, Cw30 and Cw31) for C. walkeriana. The combined power to exclude the first parent and combined non-exclusion probability of identity were 0.999 and 2.3 × 10−20, respectively. These new loci can be used in studies of germplasm resources, and assessments of genotypic and genetic diversity and population structure, thus improving the accuracy of such analyses and their applicability in the conservation and protection of these endangered species.
Private Litigation as a Foreign Relations Problem
Authors: Stephan Paul B.
Published: July 2017

In RJR Nabisco v. European Community, the Court added an exclamation point to a long term trend in its jurisprudence. It believes, this trend indicates, that private civil suits pose specific foreign relations issues, at least when the targets are foreign transactions and actors, to which the Court will respond by erecting barriers. To this general point the case adds an unsurprising, but still important codicil: These problems don’t go away when foreign states take advantage of the U.S. civil litigation system by acting as plaintiffs.
The Frankenstein’s Monster of Extraterritoriality Law
Authors: Colangelo Anthony J.
Published: July 2017

The judge-made presumption against extraterritoriality has recently become a motley patchwork of eccentric and sometimes contradictory doctrines seemingly stitched together for one, and only one, mission: to deprive plaintiffs the right to sue in U.S. courts for harms suffered abroad. It lumbers along, blithely squashing precedent, principle, statutory text, and legislative intent—all to heed its abiding and single-minded obsession. The Supreme Court has so far mangled the scope of the Securities Exchange Act and the Alien Tort Statute (ATS), and, in RJR Nabisco v. European Community, has placed another statute—The Racketeer Influenced and Corrupt Organizations Act (RICO)—on the chopping block. The major surgery performed was amputating RICO’s private right of action for extraterritorial offenses and replacing it with a much stubbier appendage limited to injuries suffered on U.S. territory.
Doubling Down on Litigation Isolationism
Authors: Bookman Pamela K.
Published: July 2017

Last year in the Stanford Law Review, I described an emerging trend in U.S. courts: litigation isolationism. Through developments in personal jurisdiction,forum non conveniens, international comity, and the presumption against extraterritoriality, I argued, courts have developed increasingly strong tools for avoiding transnational litigation. Decisions advancing litigation isolationism often fail to accomplish their stated goals—typically promoting separation of powers, avoiding interstate friction, and protecting defendants from the inconvenience of U.S. litigation. They also undermine important U.S. interests, often by excluding or dismissing cases that have close ties to the United States. At the end of that article, I cautioned against the continuation of the trend.
Out-Beale-Ing Beale
Authors: Vázquez Carlos M.
Published: July 2017

In response to the 1991 Supreme Court decision resuscitating the presumption against extraterritoriality [hereinafter “PAE” or “presumption”],EEOC v. Arabian American Oil Co.(Aramco), Larry Kramer described the presumption as an anachronism—a throwback to the strict territorialist approach to choice of law that prevailed before the mid-Twentieth Century but has been mostly abandoned since then. The title of his scathing article,Vestiges of Beale, referred to Joseph Beale, the Harvard Law professor and reporter of the First Restatement of Conflict of Laws, whose since-discredited theories underlay that Restatement’s approach to choice of law. In the cases sinceAramco, the Court has strengthened and expanded the presumption. With its decision inRJR Nabisco v. European Community, it is fair to say, the Court has out-Beale’d Beale.
The Idea of Global Public Law: Response To Unbound Symposium Essays
Authors: Hovell Devika
Published: July 2017

Law abhors a vacuum. Lawyers (including international lawyers) have constructed their profession around the fiction that such a thing is impossible. Where gaps emerge in a legal framework, lawyers face the task of filling it, compromised by the additional hurdle of having to pretend there was no gap in the first place.

The challenge has intensified with the ever-widening and deepening accountability gap that has accompanied the growth of global governance. In the period between H.G. Wells’ writing of The New World Order and the drafting of Security Council resolutions 827, 1267, 1373, and 1540, global governance has evolved from an idea of utopian/dystopian fiction to reality. In a recent article in the American Journal of International Law on “Due Process in the United Nations,” I argue that as legal academics we are justified in taking a more architectural role in proposing a legal framework to fill the good-governance-size hole in this emerging tier of governance. Essayists in the AJIL Unbound Symposium convened in response to my article raised interesting (and fairly fundamental) challenges to the methodology proposed. The hosts of the symposium kindly offered me the chance to respond—I took them up. There may be gaps in international law, but never silences.
Gender Representation on the Tribunals of the United Nations Internal Justice System: A Response to Nienke Grossman
Authors: Ebrahim-Carstens Memooda
Published: July 2017

Shylock: Most learned judge, a sentence! Come prepare!

Portia: This bond doth give thee here no jot of blood; The words expressly are “a pound of flesh.”

(The Merchant of Venice, Act 4, Scene1)

In her interesting study on “Achieving Sex Representative International Court Benches,” Nienke Grossman observes that international judgeships are often based on personal networks and social currency, or used to reward political loyalty or to advance political agendas, rather than to select the most qualified candidates. She illustrates how nomination procedures for international benches generally lack transparency, and horse-trading infects elections to the international courts. To ensure gender equity, Grossman advocates that states should take remedial steps to make nomination and selection procedures more open, transparent, and merit-based, at both the national and the international level.
Palestine and the Secret Treaties
Authors: Kattan Victor
Published: July 2017

The Sykes-Picot agreement is the foremost example of Western double-dealing in the Middle East since the discovery of oil. The agreement, formalized in an exchange of notes between the British Foreign Secretary and the French Ambassador to the United Kingdom in London, is named after its principal negotiators Sir Mark Sykes (1879-1919) and Georges-Picot (1870-1951). As one of several overlapping arrangements affecting the postwar settlement in West Asia secretly negotiated during the First World War, the agreement provided for the division of the region into spheres of influence comprised of nominally independent Arab states under the “tutelage” of British and French advisers.
Textual Settlements: The Sykes–Picot Agreement and Secret Treaty-Making
Authors: Donaldson Megan
Published: July 2017

The Sykes–Picot agreement embodies a certain style of diplomacy: an assumption of European predominance, given expression through cartographic line-drawing, terms of art (“protection,” “independence,” “interests”), and a structural secrecy which kept agreements from rival European powers, on the one hand, and from the peoples most affected, on the other. It is this element of secrecy that constitutes the focus of the present contribution. I situate the Sykes–Picot agreement in a prewar pattern of secrecy as diplomatic technique, explore its role in spurring a new regime of publicity for treaties, and take it as a touchstone for exploring whether this new regime could achieve a fundamental transformation of prevailing modes of diplomacy.
The Conflicts Restatement and the World
Authors: Michaels Ralf
Published: July 2017

Some sixteen years ago, on the occasion one of many symposia on the possibility of a new Restatement on Conflict of Laws to replace the much-derided Second Restatement, Mathias Reimann suggested that a new Restatement should focus on the requirements of what he called “the international age.” Conflict of laws is increasingly international, he pointed out. This remains true today—just recall that three of the four recent U.S. Supreme Court decisions on personal jurisdiction concerned international conflicts. A new Restatement must take that into account. Reimann formulated three very sensible wishes for drafters of a new Restatement: they should consider every rule and principle they formulate with international disputes in mind; they should work comparatively; and they should include foreign advisers.
The Inter-American Human Rights System and the Colombian Peace: Redefining the Fight Against Impunity
Authors: Acosta-López Juana Inés
Published: July 2017

The results of the plebiscite suggest that Colombia’s international obligations regarding the right to justice will be a key issue in the new postplebiscite phase of the peace negotiation. Perhaps, then, the best starting point for an analysis of these obligations is the rejected Peace Accord. If it complies with international obligations, then it seems likely that any new agreement will also be in compliance: opponents focused much of their arguments on the high level of impunity supposedly embedded in the original deal.
Gender Panic and the Failure of a Peace Agreement
Authors: Céspedes-Báez Lina M.
Published: July 2017

Gender may have been one of the main reasons behind the rejection of the Peace Agreement in Colombia. A few hours after the narrow victory of those who opposed the deal, Senator and ex-president Álvaro Uribe Vélez gave a speech calling for the strengthening of religious family values. His words echoed an argument that gained traction in recent months in Colombia, particularly in the Evangelical Christian community: that the content of the Peace Accord dismantled traditional mores, such as the biological difference between man and woman, the importance of the heterosexual family, and the place of religion in public life.
How Politics Shapes the Contributions of Justice: Lessons from the ICTY and the ICTR
Authors: Rodman Kenneth A.
Published: July 2017

The International Criminal Tribunals for the former Yugoslavia (ICTY) and Rwanda (ICTR) established a number of precedents in international criminal law, as detailed by Darryl Robinson and Gillian MacNeil.1 They also set the template for the International Criminal Court (ICC) and other tribunals as to how politics can both empower and constrain international prosecution and determine its potential contribution to peace.The lesson of the ICTY is that international criminal law can assist peace processes in an ongoing way if powerful states and international institutions complement it with coercive political strategies to weaken regimes or militias led by criminal spoilers to the point where their cooperation is not needed tonegotiate and maintain a peace settlement.The lesson of the ICTR is that the impact of international criminal law on consolidating peace is dependent upon the political agenda of the state on whose territory the crimes oc-curred and whose cooperation is needed for effective prosecution.Therefore, the contribution of prosecution to peace depends on whether the law is embedded in national and international political com-mitments that go beyond compliance with formal legal obligations and over which a tribunal has limited influence.
On a Self-Deconstructing Symposium
Authors: Moyn Samuel
Published: July 2017

It is not clear what there is left for a commentator to say once a symposium has unfolded in such a way as to cancel itself out. But in case others read it differently than I do, I am happy to explain how I think this process occurs across the wonderful though self-canceling pages of the American Journal of International Law symposium on the International Criminal Tribunal for the Former Yugoslavia (ICTY) and the International Criminal Tribunal for Rwanda (ICTR) and—through valedictory reflection on thoseenter prises—on contemporary international criminal law so far. The self-cancellation process, as I see it, takes place in the move from creation story and doctrinal evolution to impact measurement amidst legacy rhetoric. One might take this result as an index of where things stand (or whether anything stands) in the fascinating emergence of a prestigious enterprise—and what might come next.
The South China Sea Arbitration: Innovations in Marine Environmental Fact-Finding and due Diligence Obligations
Authors: Mbengue Makane Moïse
Published: July 2017

The South China Sea Arbitration is a leading case in a new generation of environmental disputes, namely, environmental disputes that occur in disputed territorial or maritime areas. The dispute between the Philippines and China before the United Nations Convention on the Law of the Sea (UNCLOS) Annex VII Tribunal (the Tribunal) dealt in significant part with the Philippines’ allegations of environmental violations by China. The Philippines asserted that China tolerated harmful fishing practices and proceeded with harmful construction activities, and that both caused serious harm to the marine environment of the South China Sea.
Mice lacking NF-κB1 exhibit marked DNA damage responses and more severe gastric pathology in response to intraperitoneal tamoxifen administration
Authors: Burkitt Michael D , Williams Jonathan M , Townsend Tristan , Hough Rachael , Pritchard D Mark
Published: July 2017

Tamoxifen (TAM) has recently been shown to cause acute gastric atrophy and metaplasia in mice. We have previously demonstrated that the outcome of Helicobacter felis infection, which induces similar gastric lesions in mice, is altered by deletion of specific NF-κB subunits. Nfkb1−/− mice developed more severe gastric atrophy than wild-type (WT) mice 6 weeks after H. felis infection. In contrast, Nfkb2−/− mice were protected from this pathology. We therefore hypothesized that gastric lesions induced by TAM may be similarly regulated by signaling via NF-κB subunits. Groups of five female C57BL/6 (WT), Nfkb1−/−, Nfkb2−/− and c-Rel−/− mice were administered 150 mg/kg TAM by IP injection. Seventy-two hours later, gastric corpus tissues were taken for quantitative histological assessment. In addition, groups of six female WT and Nfkb1−/− mice were exposed to 12 Gy γ-irradiation. Gastric epithelial apoptosis was quantified 6 and 48 h after irradiation. TAM induced gastric epithelial lesions in all strains of mice, but this was more severe in Nfkb1−/− mice than in WT mice. Nfkb1−/− mice exhibited more severe parietal cell loss than WT mice, had increased gastric epithelial expression of Ki67 and had an exaggerated gastric epithelial DNA damage response as quantified by γH2AX. To investigate whether the difference in gastric epithelial DNA damage response of Nfkb1−/− mice was unique to TAM-induced DNA damage or a generic consequence of DNA damage, we also assessed gastric epithelial apoptosis following γ-irradiation. Six hours after γ-irradiation, gastric epithelial apoptosis was increased in the gastric corpus and antrum of Nfkb1−/− mice. NF-κB1-mediated signaling regulates the development of gastric mucosal pathology following TAM administration. This is associated with an exaggerated gastric epithelial DNA damage response. This aberrant response appears to reflect a more generic sensitization of the gastric mucosa of Nfkb1−/− mice to DNA damage.
Oroxylin A activates PKM1/HNF4 alpha to induce hepatoma differentiation and block cancer progression
Authors: Wei Libin , Dai Yuanyuan , Zhou Yuxin , He Zihao , Yao Jingyue , Zhao Li , Guo Qinglong , Yang Lin
Published: July 2017

Liver cancer is the second cause of death from cancer worldwide, without effective treatment. Traditional chemotherapy for liver cancer has big side effects for patients, whereas targeted drugs, such as sorafenib, commonly have drug resistance. Oroxylin A (OA) is the main bioactive flavonoids of Scutellariae radix, which has strong anti-hepatoma effect but low toxicity to normal tissue. To date, no differentiation-inducing agents have been reported to exert a curative effect on solid tumors. Here our results demonstrated that OA restrained the proliferation and induced differentiation of hepatoma both in vitro and in vivo, via inducing a high PKM1 (pyruvate kinase M1)/PKM2 (pyruvate kinase M2) ratio. In addition, inhibited expression of polypyrimidine tract-binding protein by OA was in charge of the decrease of PKM2 and increase of PKM1. Further studies demonstrated that increased PKM1 translocated into the nucleus and bound with HNF-4α (hepatocyte nuclear factor 4 alpha) directly, promoting the transcription of HNF-4α-targeted genes. This work suggested that OA increased PKM1/PKM2 ratio, resulting in HNF-4α activation and hepatoma differentiation. Especially, OA showed reliable anticancer effect on both human primary hepatocellular carcinoma cells and patient-derived tumor xenograft model for hepatoma, and slowed down the development of primary hepatoma, suggesting that OA could be developed into a novel differentiation inducer agent for hepatoma.
FOXQ1 regulates senescence-associated inflammation via activation of SIRT1 expression
Authors: Wang Pan , Lv Cuicui , Zhang Tao , Liu Junling , Yang Jin , Guan Fangxia , Hong Tianpei
Published: July 2017

Cellular senescence is an initial barrier to tumor development that prevents the proliferation of premalignant cells. However, some of the features of senescent cells seem to promote tumor progression via senescence-associated secretory phenotype (SASP). Here, we demonstrated that the protein level of forkhead box Q1 (FOXQ1), which highly overexpresses in several kinds of tumors, was significantly downregulated during both replicative and oncogene-induced senescence. Moreover, overexpression of FOXQ1 delayed senescence, whereas FOXQ1 silence led to premature senescence in human fibroblasts. Furthermore, we identified that FOXQ1 upregulated SIRT1 expression through transcriptional regulation via directly binding to the SIRT1 promoter. Finally, we showed that FOXQ1 remarkably inhibited the replicative senescence through depressing the expression of the inflammatory cytokines interleukin-6 (IL-6) and IL-8 via modulation of SIRT1-NF-κB pathway. In addition, FOXQ1 overexpressed in human esophageal cancer cells and ablation of FOXQ1 restrained the tumourigenic ability of the esophageal cancer cells (EC109 and EC9706) in a mouse xenograft model in vivo. Taken together, these findings uncover a previously unidentified role of FOXQ1 regulating SASP and tumor development at same time.
Different Potential of Extracellular Vesicles to Support Thrombin Generation: Contributions of Phosphatidylserine, Tissue Factor, and Cellular Origin
Authors: Tripisciano Carla , Weiss René , Eichhorn Tanja , Spittler Andreas , Heuser Thomas , Fischer Michael Bernhard , Weber Viktoria
Published: July 2017

Cells release diverse types of vesicles constitutively or in response to proliferation, injury, inflammation, or stress. Extracellular vesicles (EVs) are crucial in intercellular communication, and there is emerging evidence for their roles in inflammation, cancer, and thrombosis. We investigated the thrombogenicity of platelet-derived EVs, which constitute the majority of circulating EVs in human blood, and assessed the contributions of phosphatidylserine and tissue factor exposure on thrombin generation. Addition of platelet EVs to vesicle-free human plasma induced thrombin generation in a dose-dependent manner, which was efficiently inhibited by annexin V, but not by anti-tissue factor antibodies, indicating that it was primarily due to the exposure of phosphatidylserine on platelet EVs. Platelet EVs exhibited higher thrombogenicity than EVs from unstimulated monocytic THP-1 cells, but blockade of contact activation significantly reduced thrombin generation by platelet EVs. Stimulation of monocytic cells with lipopolysaccharide enhanced their thrombogenicity both in the presence and in the absence of contact activation, and thrombin generation was efficiently blocked by anti-tissue factor antibodies. Our study provides evidence that irrespective of their cellular origin, EVs support the propagation of coagulation via the exposure of phosphatidylserine, while the expression of functional tissue factor on EVs appears to be limited to pathological conditions.
Erratum: Tactile Flow Overrides Other Cues To Self Motion
Authors: Harris Laurence R. , Sakurai Kenzo , Beaudot William H. A.
Published: July 2017

A correction to this article has been published and is linked from the HTML version of this paper. The error has been fixed in the paper.
Metabolic Biomarkers for Prognostic Prediction of Pre-diabetes: results from a longitudinal cohort study
Authors: Zeng Hailuan , Tong Renchao , Tong Wenxin , Yang Qiaoling , Qiu Miaoyan , Xiong Aizhen , Sun Siming , Ding Lili , Zhang Hongli , Yang Li , Tian Jingyan
Published: July 2017

To investigate the metabolic biomarkers of predicting the transition from pre-diabetes (pre-DM) to normal glucose regulation (NGR) and diabetes (DM) in a longitudinal cohort study. 108 participants with pre-DM were followed up for ten years and divided into 3 groups according to different glycemic outcomes. 20 participants progressed to DM, 20 regressed to NGR, and 68 remained at pre-DM. Alterations in plasma metabolites in these groups were evaluated by untargeted ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS). Twenty three metabolites related to glycerophospholipid metabolism, oxidation and antioxidation were associated with the process from pre-DM to NGR, while twenty two metabolites related to amino acid metabolism, glycerophospholipid metabolism and mitochondrial β-oxidation played important roles in the progression to DM. Results from stepwise logistic regression analysis showed that five biomarkers (20-Hydroxy-leukotriene E4, Lysopc(20:4), 5-methoxytryptamine, Endomorphin-1, Lysopc(20:3)) were good prediction for the restoration to NGR, and five biomarkers (Iso-valeraldehyde, linoleic acid, Lysopc(18:1), 2-Pyrroloylglycine, Dityrosine) for the development of DM. The findings suggest that the combination of these potential metabolites may be used for the prognosis of pre-DM. Targeting the pathways that involved in these prognostic biomarkers would be beneficial for the regression to NGR and the early prevention of DM among pre-DM.
Evidence linking microRNA suppression of essential prosurvival genes with hippocampal cell death after traumatic brain injury
Authors: Boone Deborah Kennedy , Weisz Harris A. , Bi Min , Falduto Michael T. , Torres Karen E. O. , Willey Hannah E. , Volsko Christina M. , Kumar Anjali M. , Micci Maria-Adelaide , Dewitt Douglas S. , Prough Donald S. , Hellmich Helen L.
Published: July 2017

The underlying molecular mechanisms of how dysregulated microRNAs (miRNAs) cause neurodegeneration after traumatic brain injury (TBI) remain elusive. Here we analyzed the biological roles of approximately 600 genes - we previously found these dysregulated in dying and surviving rat hippocampal neurons - that are targeted by ten TBI-altered miRNAs. Bioinformatic analysis suggests that neurodegeneration results from a global miRNA-mediated suppression of genes essential for maintaining proteostasis; many are hub genes - involved in RNA processing, cytoskeletal metabolism, intracellular trafficking, cell cycle progression, repair/maintenance, bioenergetics and cell-cell signaling - whose disrupted expression is linked to human disease. Notably, dysregulation of these essential genes would significantly impair synaptic function and functional brain connectivity. In surviving neurons, upregulated miRNA target genes are co-regulated members of prosurvival pathways associated with cellular regeneration, neural plasticity, and development. This study captures the diversity of miRNA-regulated genes that may be essential for cell repair and survival responses after TBI.
The Association of Ambient Temperature and Violent Crime
Authors: Tiihonen Jari , Halonen Pirjo , Tiihonen Laura , Kautiainen Hannu , Storvik Markus , Callaway James
Published: July 2017

It is controversial if global warming will result into increased crime and conflict rate, and no causal neurobiological mechanisms have been proposed for the putative association between ambient temperature and aggressive behavior. This study shows that during 1996–2013, ambient temperature explained 10% of variance in the violent crime rate in Finland, corresponding to a 1.7% increase/degree centigrade. Ambient temperature also correlated with a one month delay in circannual changes in peripheral serotonin transporter density among both offenders and healthy control subjects, which itself correlated strongly with the monthly violent crime rate. This suggests that rise in temperature modulates serotonergic transmission which may increase impulsivity and general human activity level, resulting into increase in social interaction and risk of violent incidents. Together, these results suggest that the effect of ambient temperature on occurrence of violent crime is partly mediated through the serotonergic system, and that a 2 °C increase in average temperatures would increase violent crime rates by more than 3% in non-tropical and non-subtropical areas, if other contributing factors remained constant.
The Epigenetic Landscape of Promoter Genome-wide Analysis in Breast Cancer
Authors: Karsli-Ceppioglu Seher , Dagdemir Aslihan , Judes Gaëlle , Lebert André , Penault-Llorca Frédérique , Bignon Yves-Jean , Bernard-Gallon Dominique
Published: July 2017

Breast cancer is a heterogeneous disease due to its clinico-pathological features and response to therapy. The classification of breast tumors based on their hormone receptor status and pathologic features. Post-translational histone modifications come into prominence for regulation of gene expression in cancer pathogenesis. Here, we analyzed dysregulation of H3K9ac and H3K27me3-enriched subtype-specific genes using ChIP-on-chip assay in breast cancer tumors and matched normal tissue samples. Breast cancer tumors were classified according to St Gallen Consensus 2013. Our results indicated that the promoter regions of genes modified by H3K9ac epi-mark are commonly associated with tumors with HER2-positive and TNBC subtype. H3K27me3-enriched genes were comprised of Luminal A and B1 subtypes. We constructed a network structure to elicit epigenetically regulated genes related with breast cancer progression. The central genes of the network (RUNX1, PAX3, GATA4 and DLX5) were subjected for epigenetically dysregulation in association with different breast cancer subtypes. Our study submits epigenetic mechanisms are crucial to elicit subtype-specific regulation in breast cancer and ChIP-on-chip assay provides a better understanding for breast tumorigenesis and new approaches for prevention and treatment.
Dietary patterns reflecting healthy food choices are associated with lower serum LPS activity
Authors: Ahola Aila J , Lassenius Mariann I. , Forsblom Carol , Harjutsalo Valma , Lehto Markku , Groop Per-Henrik
Published: July 2017

Gram-negative bacteria-derived lipopolysaccharides (LPS) are associated with various negative health effects. Whether diet is associated with LPS, is an understudied phenomenon. We investigated the association between diet and serum LPS activity in 668 individuals with type 1 diabetes in the FinnDiane Study. Serum LPS activity was determined using the Limulus Amoebocyte Lysate assay. Diet was assessed with a food frequency questionnaire (FFQ) section of a diet questionnaire and a food record. The food record was used to calculate energy, macronutrient, and fibre intake. In a multivariable model, energy, macronutrient, or fibre intake was not associated with the LPS activity. Using factor analysis, we identified seven dietary patterns from the FFQ data (“Sweet”, “Cheese”, “Fish”, “Healthy snack”, “Vegetable”, “Traditional”, and “Modern”). In a multivariable model, higher factor scores of the Fish, Healthy snack, and Modern patterns predicted lower LPS activity. The validity of the diet questionnaire was also investigated. The questionnaire showed reasonable relative validity against a 6-day food record. The two methods classified participants into the dietary patterns better than expected by chance. In conclusion, healthy dietary choices, such as consumption of fish, fresh vegetables, and fruits and berries may be associated with positive health outcomes by reducing systemic endotoxaemia.
The Distribution Characteristics and Applications for Maternal Cells on Chicken Egg Vitelline Membrane
Authors: Li Quanlin , Li Wenbo , Li Xingzheng , Liu Lulu , Zhang Ying , Guo Yuying , Chen Xia , Xu Guiyun , Zheng Jiangxia
Published: July 2017

The major components of vitelline membrane (VM) are ovomucin, VM outer (VMO) I and VMO II. At present, the distribution pattern of maternal cells on the VM has not been described in detail. In this study, the existence and distribution characteristics of maternal cells on VM were observed. There were more than 3.2 × 105 somatic cells on VM, which were uneven distributed. The calcein AM/PI staining of the maternal cells on the VM showed that the cells’ viability changed with the freshness of the eggs, and that the maternal cells gradually underwent apoptosis and became degraded. The results of morphology of different tissues indicated that the most of maternal cells on the VM were granulosa cells. Moreover, the karyotype of the cultured granulosa cells, which is the main source of cells on VM, were identified as the normal diploid karyotype of chicken. Furthermore, the VM DNA extracted from chickens and quails, which represent the eggs of different size, was adequate for further genetic analysis. The VM DNA was easily accessible and relatively constant, without cross-contamination. Therefore, the VM DNA could potentially be applied for the molecular traceability between eggs and chickens, and be beneficial in avian ecology research studies.