bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Anxiety About COVID-19 Infection in the Workplace on the Association Between Job Demands and Psychological Distress
Authors: Eguchi Hisashi , Hino Ayako , Inoue Akiomi , Tsuji Mayumi , Tateishi Seiichiro , Ando Hajime , Nagata Tomohisa , Matsuda Shinya , Fujino Yoshihisa
Published: October 2021

Purpose: There is limited information about the association between workplace psychosocial factors and general worker mental health status during the COVID-19 pandemic. In the present study, we examined how anxiety about being infected by COVID-19 in the workplace affected the association between job demands and psychological distress (PD).

Method: A cross-sectional online survey was conducted in December 2020. The final analyzed sample was 27,036. The dependent variable of PD was assessed using the Kessler Psychological Distress Scale (K6). Job demands were assessed using the Job Content Questionnaire. Feelings of anxiety were assessed by participants' responses to the following question: “Do you feel anxiety about being infected by COVID-19 in the workplace?” We used a two-level regression adjusting for prefectural level: each individual-level variable at level 1 was nested into each prefecture at level 2, stratified by presence of anxiety.

Results: A total of 50.5% of participants felt anxious about being infected by COVID-19 in the workplace. The interaction between anxiety and job demands was significant. Job demands were positively associated with PD. In the stratified analysis, the associations were stronger among employees who experienced anxiety about COVID-19 infection in the workplace than among those who did not.

Conclusion: The association between job demands and PD may be strengthened by anxiety about COVID-19 infection in the workplace.
Uneven Use of Remote Work to Prevent the Spread of COVID-19 in South Korea's Stratified Labor Market
Authors: Park Saejung , Lee Sanghee , Cho Joonmo
Published: October 2021

Background: This research analyzed whether South Korean companies adopted remote work during the COVID-19 pandemic by focusing on the dual labor market structure comprising of primary sector (large corporations) and secondary sector [small and medium enterprises (SMEs)]. Companies in the dual labor market were classified based on firm size.

Methods: We used August supplementary data from the Economically Active Population Survey covering 2017–2020 provided by Statistics Korea. In this empirical study, a Linear Probability Model was used to analyze the probability that employees would work for companies that introduced remote work since COVID-19 depending on the size of the company.

Results: This study showed three main results. First, unlike other flexible work systems, the use of remote work has increased rapidly since COVID-19. Second, the larger the size of the company, the higher the probability that employees would work for companies that introduced remote work after COVID-19. Third, according to the analysis by industry, the difference in remote work utilization between large corporations and SMEs was relatively small because of a similar working method in manufacturing.

Conclusion: Results of this study suggested that polarization within the dual labor market structure also spilled over to adoption of remote work, which was initially introduced to prevent the spread of the pandemic. This study examined the system and factors of labor-management relations contributing to such polarization and presented policy directions for the current labor market structure.
The Contribution of Community Health Education to Sustainable Control of the Neglected Zoonotic Diseases
Authors: Butala Caitlin , Fyfe Jenna , Welburn Susan Christina
Published: October 2021

Effective and sustainable control of the Neglected Zoonoses (NZDs) demands a One Health approach. NZDs largely impact on individuals in low- and middle-income countries, disproportionally affecting resource poor communities with poor access to veterinary and human health services and to clean water and which are intrinsically dependent on animals for their livelihoods. Many NZDs in humans can be treated, but treatment is often complex and expensive. Similarly, while tools for prevention of transmission may exist, they are complex and expensive to adopt at the scale required to be effective. The cost of intervention for NZDs is high when compared to the public health benefits alone, but costs are easily outweighed by full cross sector analysis and when monetary and non-monetary benefits to all stakeholders are considered. Education is a key tool, often overlooked in favor of more complex solutions for the control of NZDs. Successful education programs have been targeted to children of school age for Taenia solium in Kenya, schistosomiasis in Nigeria, and soil transmitted helminths in China. A Snakes and Ladders board game, designed to teach children about schistosomiasis and encourage compliance with mass deworming programs, deployed in Nigerian schools, showed a 67% increase in knowledge of praziquantel and 65% of children who had previously rejected treatment requested the drug at school. For soil transmitted helminths in China, presentation of health information in cartoon format rather than in poster format, showed post-assessment knowledge to be 90% higher. With the rise in affordable smart-phone technology, internet access and airtime in communities in low- and middle- income countries e-education is an increasingly attractive proposition as an intervention tool for the NZDs. The Vicious Worm, a computer based educational health tool that has been designed around the prevention of Taenia Solium has shown remarkable efficacy in affected communities in which it has been deployed with participants applying the principles learned in their communities. This review explores the successes and benefits of education as a control tool for the NZDs.
Experimental Evaluation of Tactile Sensors for Compliant Robotic Hands
Authors: Friedl Werner A. , Roa Máximo A.
Published: October 2021

The sense of touch is a key aspect in the human capability to robustly grasp and manipulate a wide variety of objects. Despite many years of development, there is still no preferred solution for tactile sensing in robotic hands: multiple technologies are available, each one with different benefits depending on the application. This study compares the performance of different tactile sensors mounted on the variable stiffness gripper CLASH 2F, including three commercial sensors: a single taxel sensor from the companies Tacterion and Kinfinity, the Robotic Finger Sensor v2 from Sparkfun, plus a self-built resistive 3 × 3 sensor array, and two self-built magnetic 3-DoF touch sensors, one with four taxels and one with one taxel. We verify the minimal force detectable by the sensors, test if slip detection is possible with the available taxels on each sensor, and use the sensors for edge detection to obtain the orientation of the grasped object. To evaluate the benefits obtained with each technology and to assess which sensor fits better the control loop in a variable stiffness hand, we use the CLASH gripper to grasp fruits and vegetables following a published benchmark for pick and place operations. To facilitate the repetition of tests, the CLASH hand is endowed with tactile buttons that ease human–robot interactions, including execution of a predefined program, resetting errors, or commanding the full robot to move in gravity compensation mode.
Nitrogen and Phosphorus Balances Vary at the Whole-Farm, Field, and Within-Field Scales
Authors: Berlingeri Jonathan M. , Lawrence Joseph R. , Sunoj S. , Czymmek Karl J. , Ketterings Quirine M.
Published: October 2021

A field nutrient balance (supplied minus harvested) can be an effective, end-of-season management evaluation tool. However, development of guidance for balance-based management requires knowledge of variability in balance inputs. To contribute to development of such guidelines, we evaluated the impact of corn silage hybrid selection, nutrient management, and growing conditions on field nitrogen (N) balances and documented variability in N and phosphorus (P) balances at the whole-farm, field, within-field levels. Variability in N removal among hybrids was evaluated using hybrid trials (5 locations, 4 years each). Variability in farm and field balances (4 farms, 2 years each) and within-field balances (2 farms, 2 years each) was assessed as well. Nitrogen supply comprised soil N (soil type-specific book values), rotation N, past manure N, and current year N (fertilizer and/or manure). Total N balances included all current year manure N while available N balances considered only plant-available N from manure. Phosphorus balances were derived as total P applied minus P harvested. Yield explained 81% of the variability in N uptake across hybrids. Nitrogen uptake intensity (NUI; N uptake per unit of yield) varied across locations and years, averaging 4.3 ± 0.1 kg N/Mg for short-season hybrids [≤95 days-to-maturity (DTM)] vs. 4.1 ± 0.1 kg N Mg−1 for longer-season hybrids. Whole-farm N balances ranged from 139 to 251 kg N ha−1 for total N and 43 to 106 kg N ha−1 for available N. Phosphorus balances ranged from 28 to 154 kg P ha−1. Balances per field ranged from −8 to 453, −66 to 250 kg N ha−1, and −30 to 315 kg P ha−1 for total N, available N, and total P, respectively, while within-field balances showed even larger ranges. We conclude that (1) variability in corn silage N and P balances at field and within-field scales and across year is large, emphasizing the need for field and within-field (where feasible) evaluation tools and management options, and (2) feasible limits for N balances should include both total and available N.
Who Governs at What Price? Technocratic Dominance, Ways of Knowing, and Long-Term Resilience of Brazil's Water System
Authors: Almazán-Casali Stefania , Puga Bruno Peregrina , Lemos Maria Carmen
Published: October 2021

Technocratic decision making has been long criticized for dampening participation and limiting the range of adaptive choices through its overreliance on infrastructure-based solutions. There has been growing attention to how technocratic approaches shape long-term resilience of water systems, especially under the threat of climatic change impacts. In Brazil, even under its highly decentralized and participatory water management system, technical expertise and science-based decisions have been often promoted as a desirable mechanism to insulate governance outcomes from the country's prevailing clientelistic and rent-seeking politics. Yet, Brazilian river basins continue to struggle with long-standing problems (such as universal access to sanitation) and increasing challenges for guaranteeing water provision under recurrent drought. In this study, we examine how technocratic insulation, different ways of knowing (WoKs), and participatory governance shape long-term resilience in one of Brazil's most important river basins, the Piracicaba-Capivari-Jundiaí (PCJ). By taking an in-depth look at how the PCJ River Basin's governance system responded to the 2014 Brazilian water crisis, we seek to understand how planning decisions in the aftermath of the crisis were influenced by different actors, and how the outcomes of those decisions are likely to shape long term resilience. Based on 27 in-depth interviews with members of the PCJ River Basin Committees, we show how a distinct preference for infrastructure-based solutions to deal with on-going and upcoming challenges may be unsustainable under climate change as the basin's traditional technocratic approach failed both to insulate its decisions from politics and to explore adaptive water management solutions that might be key to shape long-term resilience.
The Cod and the Cut: Intra-Active Intuitions
Authors: Hertz Tilman , Mancilla Garcia Maria
Published: October 2021

Interest in causality is growing in sustainability science and it has been argued that a multiplicity of approaches is needed to account for the complexities of social-ecological dynamics. However, many of these approaches operate within perspectives that establish a separation between what has causal agency and all the rest, which is relegated to the role of background conditions. We argue that the distinction between causal elements and background conditions is by no means a necessary one, and that the causal agency of background conditions is worthy of investigation. We argue that such conditions correspond to what Karen Barad has called a “cut”: a specific determination of the world (or part of it) respective to another part, for which it becomes intelligible. In this sense, most approaches to causality so far operate from “within” particular cuts. To illustrate this, we focus on the paradigmatic case of the Baltic cod collapse in the eighties. This case has been extensively studied, and overfishing has been identified as a key cause explaining the collapse. We dig deeper into the conditions which characterized fishing practices in the run-up to the collapse and uncover the separation between the social and the ecological that they enforce by encouraging policies to increase productivity under the rationale of national “development”. We then re-examine the case from a process-relational perspective, rejecting the separation of nature from society. A process-relational perspective allows us to consider relations as constitutive of processes through which what exists becomes determinate. For this purpose we use the concepts of intra-action (co-constitution of processes) and of performativity (determination of language and matter within processes). We complete our conceptual framework by drawing inspiration from pragmatist philosophers and suggest that the concept of intuition can constitute an alternative to untangle causal dynamics and explain social-ecological phenomena beyond the cause/condition dichotomy. This article seeks to fulfil two objectives: firstly, to question the thick boundaries between conditions and causal elements that explain the processes in which social-ecological systems evolve; secondly, to provide a different approach to transforming a social-ecological system.
To Other Planets With Upgraded Millennial Kombucha in Rhythms of Sustainability and Health Support
Authors: Kozyrovska Natalia , Reva Oleg , Podolich Olga , Kukharenko Olga , Orlovska Iryna , Terzova Vitalia , Zubova Ganna , Trovatti Uetanabaro Ana Paula , Góes-Neto Aristóteles , Azevedo Vasco , Barh Debmalya , Verseux Cyprien , Billi Daniela , Kołodziejczyk Agata Maria , Foing Bernard , Demets René , Vera Jean-Pierre de
Published: October 2021

Humankind has entered a new era of space exploration: settlements on other planetary bodies are foreseen in the near future. Advanced technologies are being developed to support the adaptation to extraterrestrial environments and, with a view on the longer term, to support the viability of an independent economy. Biological processes will likely play a key role and lead to the production of life-support consumables, and other commodities, in a way that is cheaper and more sustainable than exclusively abiotic processes. Microbial communities could be used to sustain the crews’ health as well as for the production of consumables, for waste recycling, and for biomining. They can self-renew with little resources from Earth, be highly productive on a per-volume basis, and be highly versatile—all of which will be critical in planetary outposts. Well-defined, semi-open, and stress-resistant microecosystems are particularly promising. An instance of it is kombucha, known worldwide as a microbial association that produces an eponymous, widespread soft drink that could be valuable for sustaining crews’ health or as a synbiotic (i.e., probiotic and prebiotic) after a rational assemblage of defined probiotic bacteria and yeasts with endemic or engineered cellulose producers. Bacterial cellulose products offer a wide spectrum of possible functions, from leather-like to innovative smart materials during long-term missions and future activities in extraterrestrial settlements. Cellulose production by kombucha is zero-waste and could be linked to bioregenerative life support system (BLSS) loops. Another advantage of kombucha lies in its ability to mobilize inorganic ions from rocks, which may help feed BLSS from local resources. Besides outlining those applications and others, we discuss needs for knowledge and other obstacles, among which is the biosafety of microbial producers.
The Ideal Body: A Social Construct? Reflections on Body Pressure and Body Ideal Among Students in Upper Secondary School
Authors: Sæle Ove Olsen , Sæther Ida Kathrine , Viig Nina Grieg
Published: October 2021

Several studies show that young people today are negatively impacted by body image ideals in social media. We studies young people's reflections on body image and body pressure. More precisely: How does a selected group of third-year upper secondary school students understand their body images and body pressures through social media? Eight third-year students, four of each gender, were interviewed from two upper secondary schools in a medium-sized city in Norway. An interesting find was that body pressure was not experienced solely as one-sided pressure exerted externally by media sources, but that they also personally influenced others through their own active use of social media channels like Facebook and Instagram. They reported having experienced body pressure in their own lives and in their immediate environment, and that both genders are affected. The study builds on sociocultural body theory based on Foucault's ideas and work, but also uses more recent media theory for the analysis and discussion. A BOPS model developed by the researchers was used for the operational parameters that is centered around the concepts of panopticon, synopticon and omniopticon.
From Microns to Meters: Exploring Advances in Legume Microbiome Diversity for Agroecosystem Benefits
Authors: Schaedel Marie , Hidrobo Gabriela , Grossman Julie
Published: October 2021

Legumes are of primary importance for agroecosystems because they provide protein-rich foods and enhance soil fertility through fixed atmospheric nitrogen. The legume-rhizobia symbiosis that makes this possible has been extensively studied, from basic research on biochemical signaling to practical applications in cropping systems. While rhizobia are the most-studied group of associated microorganisms, the functional benefit they confer to their legume hosts by fixing nitrogen is not performed in isolation. Indeed, non-rhizobia members of the rhizosphere and nodule microbiome are now understood to contribute in multiple ways to nodule formation, legume fitness, and other agroecosystem services. In this review, we summarize advances contributing to our understanding of the diversity and composition of bacterial members of the belowground legume microbiome. We also highlight applied work in legume food and forage crops that link microbial community composition with plant functional benefits. Ultimately, further research will assist in the development of multi-species microbial inoculants and cropping systems that maximize plant nutrient benefits, while reducing sources of agricultural pollution.
Effects of Tranexamic Acid on Bleeding in Pediatric Surgeries: A Systematic Review and Meta-Analysis
Authors: Wei Yiyong , Zhang Yajun , Jin Tao , Wang Haiying , Li Jia , Zhang Donghang
Published: October 2021

Background: Major pediatric surgeries can cause severe intraoperative blood loss. This meta-analysis aims to evaluate the efficacy of tranexamic acid (TXA) in pediatric surgeries.

Methods: We searched PubMed, Embase, Web of Science, and Cochrane Library from the conception to March 31, 2021 to identify eligible randomized controlled trials (RCTs) that evaluated the efficacy of TXA in pediatric surgeries. Two reviewers choosed studies, evaluated quality, extracted data, and assessed the risk of bias independently. Mean difference (MD) was calculated as the summary statistic for continuous data. We used a random-effects model to measure mean effects. Data were generated from the corresponding 95% confidence interval (CI) using RevMan 5.3 software. Primary outcomes included intraoperative and postoperative blood loss, red blood cell (RBC) transfusion as well as fresh frozen plasma (FFP) transfusion.

Results: Fifteen studies enrolling 1,332 patients were included in this study. The pooled outcomes demonstrated that TXA was associated with a decreased intraoperative (MD = −1.57 mL/kg, 95% CI, −2.54 to −0.60, P = 0.002) and postoperative (MD = −7.85 mL/kg, 95% CI, −10.52 to −5.19, P < 0.001) blood loss, a decreased intraoperative (MD = −7.08 mL/kg, 95% CI, −8.01 to −6.16, P < 0.001) and postoperative (MD = −5.30 mL/kg, 95% CI, −6.89 to −3.70, P < 0.001) RBC transfusion, as well as a decreased intraoperative (MD = −2.74 mL/kg, 95% CI, −4.54 to −0.94, P = 0.003) and postoperative (MD = −6.09 mL/kg, 95% CI, −8.26 to −3.91, P < 0.001) FFP transfusion in pediatric surgeries. However, no significant difference was noted between two groups in duration of surgery (MD = −12.51 min, 95% CI −36.65 to 11.63, P = 0.31). Outcomes of intraoperative and postoperative blood loss and the duration of surgery in included studies were not pooled due to the high heterogeneity.

Conclusion: This meta-analysis demonstrated that TXA was beneficial for bleeding in pediatric surgeries.
Genotypic Characterization of Mycobacterium bovis Isolates From Dairy Cattle Diagnosed With Clinical Tuberculosis
Authors: de Melo Elizabeth Hortêncio , Gomes Harrison Magdinier , Suffys Philip Noel , Lopes Márcia Quinhones Pires , de Figueiredo Teixeira Raquel Lima , dos Santos Ícaro Rodrigues , Franco Marília Masello Junqueira , Langoni Helio , Paes Antonio Carlos , Afonso José Augusto Bastos , de Mendonça Carla Lopes
Published: October 2021

Molecular diagnosis of bovine tuberculosis plays an essential role in the epidemiological knowledge of the disease. Bovine tuberculosis caused by Mycobacterium bovis represents a risk to human health. This study aimed to perform the genotypic characterization of M. bovis isolated from bovines diagnosed as tuberculosis from dairy herds in the state of Pernambuco, Brazil. Granulomas from 30 bovines were sent for microbiological culture, and colonies compatible with Mycobacterium spp. were obtained in at least one culture from 17/30 granulomas. All isolates were confirmed to be M. bovis by spoligotyping and 24loci MIRU-VNTR typing. While spoligotyping characterized the isolates as SB0121, SB0295, SB0852, SB0120, and an unclassified genotype, 24loci MIRU-VNTR rendered two clusters of two isolates each and 13 unique profiles. Loci ETR-A showed higher discriminatory power, and loci (ETR-B, ETR-C, MIRU16, MIRU27, and QUB26) showed moderate allelic diversity. This is the first study on the genetic variability of the infectious agent cause of bovine TB in Pernambuco and demonstrates variability of strains in the state. Thus, it corroborates the importance of this microorganism as agent of bovine tuberculosis and its zoonotic potential, this epidemiological tool being a determinant in the rigor of the sanitary practices of disease control in dairy herds.
In Gastric Cancer Patients Receiving Neoadjuvant Chemotherapy Systemic Inflammation Response Index is a Useful Prognostic Indicator
Authors: Chen Li , Chen Yong , Zhang Lele , Xue Yingwei , Zhang Shiwei , Li Xingrui , Song Hongjiang
Published: October 2021

Background: The preoperative systemic inflammation response index (SIRI), based on peripheral neutrophil (N), monocyte (M), and lymphocyte (L) counts, has shown mounting evidence as an effective prognostic indicator in some malignant tumors. The aim of the present study was to evaluate the prognostic significance of pre-treatment SIRI in gastric cancer patients who received neoadjuvant chemotherapy (NACT).

Methods: This retrospective study comprised 107 patients with advanced gastric cancer treated with NACT between July 2007 and September 2015 in our hospital. SIRI was calculated from peripheral venous blood samples obtained prior to treatment. The best cutoff value for SIRI by receiver operating characteristic (ROC) curve was 1.2 (low SIRI <1.21, high SIRI ≥1.21). The clinical outcomes of disease-free survival (DFS) and overall survival (OS) were analyzed by Kaplan-Meier survival analysis and compared using the log-rank test. Univariate and multivariate analyses were performed by the Cox proportional hazards regression model.

Results: The results demonstrated that the low SIRI group was statistically associated with gender, primary tumor site, white blood cell, neutrophil, and monocyte counts, NLR (neutrophil to lymphocyte ratio), MLR (monocyte to lymphocyte ratio), and PLR (platelet to lymphocyte ratio). The SIRI was predictive for DFS and OS by univariate and multivariate analysis; the low SIRI group had better median DFS and OS than the high SIRI group (median DFS 27.03 vs. 22.33 months, median OS 29.73 vs. 24.43 months). The DFS and OS in the low SIRI group were longer than the high SIRI group.

Conclusions: SIRI may qualify as a useful, reliable, and convenient prognostic indicator in patients with advanced gastric cancer to help physicians to provide personalized prognostication for gastric cancer patients treated with NACT.
High Speed Microactuators for Low Aspect Ratio High Speed Micro Aircraft Surfaces
Authors: Barrett-Gonzalez Ronald , Wolf Nathan
Published: October 2021

This paper covers a class of actuators for modern high speed, high performance subscale aircraft. The paper starts with an explanation of the challenges faced by micro aircraft, including low power, extremely tight volume constraints, and high actuator bandwidth requirements. A survey of suitable actuators and actuator materials demonstrates that several classes of piezoceramic actuators are ideally matched to the operational environment. While conventional, linear actuation of piezoelectric actuators can achieve some results, dramatic improvements via reverse-biased spring mechanisms can boost performance and actuator envelopes by nearly an order of magnitude. Among the highest performance, low weight configurations are post-buckled precompressed (PBP) actuator arrangements. Analytical models display large deflections at bandwidths compatible with micro aircraft flight control speed requirements. Bench testing of an example PBP micro actuator powered low aspect ratio flight control surface displays +/−11° deflections through 40 Hz, with no occupation of volume within the aircraft fuselage and good correlation between theory and experiment. A wind tunnel model of an example high speed micro aircraft was fabricated along with low aspect ratio PBP flight control surfaces, demonstrating stable deflection characteristics with increasing speed and actuator bandwidths so high that all major aeromechanical modes could be easily controlled. A new way to control such a PBP stabilator with a Limit Dynamic Driver is found to greatly expand the dynamic range of the stabilator, boosting the dynamic response of the stabilator by more than a factor of four with position feedback system engaged.
Hybrid Neuromuscular Training Improves Cardiometabolic Health and Alters Redox Status in Inactive Overweight and Obese Women: A Randomized Controlled Trial
Authors: Batrakoulis Alexios , Jamurtas Athanasios Z. , Draganidis Dimitrios , Georgakouli Kalliopi , Tsimeas Panagiotis , Poulios Athanasios , Syrou Niki , Deli Chariklia K. , Papanikolaou Konstantinos , Tournis Symeon , Fatouros Ioannis G.
Published: October 2021

This randomized controlled trial investigated the effects of a 5-month high-intensity hybrid-type neuromuscular training program with nontraditional implements on cardiometabolic health, redox status, and cardiovascular disease (CVD) risk in inactive overweight and obese women. Forty-nine inactive female participants with overweight and obesity (age: 36.4 ± 4.4 years; BMI: 29.1 ± 2.9 kg/m2) were randomly assigned to either a control (C, n = 21) or a training group (TR, n = 28). TR followed a 20-week supervised, progressive, time-efficient (3 days/week; 6–15 min net exercise time) program implementing loaded fundamental movement patterns with prescribed work-to-rest time intervals (20–40 s, 1:2, 1:1, 2:1) in a circuit fashion (2–3 rounds). Cardiometabolic risk factors were measured at baseline and post-training as secondary outcomes of a larger randomized controlled trial. At post-intervention, TR demonstrated favorable changes in resting heart rate (−7%, p = 0.043), high-density lipoprotein (+18.1%, p = 0.029), atherogenic index (−17%, p = 0.045), mean arterial pressure (−4.5%, p = 0.03), waist circumference (−6.2%, p = 0.005), waist-to-hip ratio (−4.6%; p = 0.015), metabolic syndrome severity score (−222%, p = 0.024), full 30-year CVD risk (−15.8%, p = 0.002) and hard 30-year CVD risk (−17.6%, p = 0.01), vascular age (−7.8%, p = 0.002), protein carbonyls (−45.7%, p = 0.001), catalase activity (+15.2%, p = 0.023), and total antioxidant capacity (+11.4%, p = 0.002) relative to C. Additionally, TR induced beneficial changes in fasting glucose (−3.4%, p = 0.002), homeostatic model assessment for insulin resistance (−15.7%, p < 0.001), diastolic blood pressure (−5.6%, p < 0.001), reduced glutathione (+39.8%, p < 0.001), 10-year CVD risk (−17.4%, p = 0.011), and total bilirubin (−21.7%, p < 0.001) compared to baseline. These results suggest that hybrid-type neuromuscular training may improve aspects of cardiometabolic health and antioxidant status in inactive overweight and obese women providing a time-efficient (~100 min/week) exercise approach in a real-world gym setting.
The Human NUP58 Nucleoporin Can Form Amyloids In Vitro and In Vivo
Authors: Danilov Lavrentii G. , Moskalenko Svetlana E. , Matveenko Andrew G. , Sukhanova Xenia V. , Belousov Mikhail V. , Zhouravleva Galina A. , Bondarev Stanislav A.
Published: October 2021

Amyloids are fibrillar protein aggregates with a cross-β structure and unusual features, including high resistance to detergent or protease treatment. More than two hundred different proteins with amyloid or amyloid-like properties are already known. Several examples of nucleoporins (e.g., yeast Nup49, Nup100, Nup116, and human NUP153) are supposed to form amyloid fibrils. In this study, we demonstrated an ability of the human NUP58 nucleoporin to form amyloid aggregates in vivo and in vitro. Moreover, we found two forms of NUP58 aggregates: oligomers and polymers stabilized by disulfide bonds. Bioinformatic analysis revealed that all known orthologs of this protein are potential amyloids which possess several regions with conserved ability to aggregation. The biological role of nucleoporin amyloid formation is debatable. We suggest that it is a rather abnormal process, which is characteristic for many proteins implicated in phase separation.
Feasibility of Photodynamic Therapy for Glioblastoma with the Mitochondria-Targeted Photosensitizer Tetramethylrhodamine Methyl Ester (TMRM)
Authors: Vasilev Alex , Sofi Roba , Smith Stuart J. , Rahman Ruman , Teschemacher Anja G. , Kasparov Sergey
Published: October 2021

One of the most challenging problems in the treatment of glioblastoma (GBM) is the highly infiltrative nature of the disease. Infiltrating cells that are non-resectable are left behind after debulking surgeries and become a source of regrowth and recurrence. To prevent tumor recurrence and increase patient survival, it is necessary to cleanse the adjacent tissue from GBM infiltrates. This requires an innovative local approach. One such approach is that of photodynamic therapy (PDT) which uses specific light-sensitizing agents called photosensitizers. Here, we show that tetramethylrhodamine methyl ester (TMRM), which has been used to asses mitochondrial potential, can be used as a photosensitizer to target GBM cells. Primary patient-derived GBM cell lines were used, including those specifically isolated from the infiltrative edge. PDT with TMRM using low-intensity green light induced mitochondrial damage, an irreversible drop in mitochondrial membrane potential and led to GBM cell death. Moreover, delayed photoactivation after TMRM loading selectively killed GBM cells but not cultured rat astrocytes. The efficacy of TMRM-PDT in certain GBM cell lines may be potentiated by adenylate cyclase activator NKH477. Together, these findings identify TMRM as a prototypical mitochondrially targeted photosensitizer with beneficial features which may be suitable for preclinical and clinical translation.
Investigation of Escherichia coli O157:H7 Survival and Interaction with Meal Components during Gastrointestinal Digestion
Authors: de La Pomelie Diane , Leroy Sabine , Talon Régine , Ruiz Philippe , Gatellier Philippe , Santé-Lhoutellier Véronique
Published: October 2021

Escherichia coli O157:H7 is responsible for foodborne poisoning, incriminating contaminated animal food and especially beef meat. This species can survive in the digestive tract, but, up to now, very few studies have considered its survival during the gastrointestinal digestion of meat. The present study aimed to investigate the survival of the pathogenic strain E. coli O157:H7 CM454 during the gastrointestinal digestion of ground beef meat and its interactions with meal components using a semidynamic digestive model. The CM454 strain in meat survived throughout digestion despite acidic pH (pH 2) and the presence of bile salts. The addition of nitrite and ascorbate in the digestion medium led to a decrease in strain survival. During digestion, a release of free iron was observed, which was accentuated in the presence of the CM454 strain. In addition, the strain modified the Fe2+/Fe3+ ratio, in favor of Fe2+ compared to the noninoculated meat sample. In the presence of nitrite, nitroso compounds such as nitrosamines, nitrosothiols, and nitrosylheme were formed. E. coli O157:H7 CM454 had no impact on N-nitrosation but seemed to decrease S-nitrosation and nitrosylation.
Application of Natural Preservatives for Meat and Meat Products against Food-Borne Pathogens and Spoilage Bacteria: A Review
Authors: Yu Hwan Hee , Chin Young-Wook , Paik Hyun-Dong
Published: October 2021

Meat and meat products are excellent sources of nutrients for humans; however, they also provide a favorable environment for microbial growth. To prevent the microbiological contamination of livestock foods, synthetic preservatives, including nitrites, nitrates, and sorbates, have been widely used in the food industry due to their low cost and strong antibacterial activity. Use of synthetic chemical preservatives is recently being considered by customers due to concerns related to negative health issues. Therefore, the demand for natural substances as food preservatives has increased with the use of plant-derived and animal-derived products, and microbial metabolites. These natural preservatives inhibit the growth of spoilage microorganisms or food-borne pathogens by increasing the permeability of microbial cell membranes, interruption of protein synthesis, and cell metabolism. Natural preservatives can extend the shelf-life and inhibit the growth of microorganisms. However, they can also influence food sensory properties, including the flavor, taste, color, texture, and acceptability of food. To increase the applicability of natural preservatives, a number of strategies, including combinations of different preservatives or food preservation methods, such as active packaging systems and encapsulation, have been explored. This review summarizes the current applications of natural preservatives for meat and meat products.
Validity and Reliability of the Korean Version of the Acceptance and Action Questionnaire-Stigma (AAQ-S-K)
Authors: Lee Hyunjin , Kwon Myoungjin , Seo Kawoun
Published: October 2021

This study investigated the validity and reliability of the Korean version of the Acceptance and Action Questionnaire-Stigma (AAQ-S-K) in people with diabetes. A total of 208 patients with diabetes participated in the study. After performing forward and backward translation of the original version of the AAQ-S into Korean, its validity (construct and concurrent validity) and reliability were assessed. Construct validity measured using confirmatory factor analysis showed a good fit. Concurrent validity was confirmed through the significant correlation between the AAQ-S-K, acceptance and action, diabetes self-stigma and diabetes distress. The AAQ-S-K was positively correlated with acceptance and action and had a negative correlation with diabetes self-stigma and diabetes distress. The reliability of the AAQ-S-K ranged from 0.82 to 0.88. The AAQ-S-K can be applied to assess stigma acceptance and action in Korean patients with diabetes and to compare the level of psychological flexibility of patients with diabetes internationally.
Portuguese Medical Students’ Interest for Science and Research Declines after Freshman Year
Authors: Pereira Margarida , Correia Gustavo , Severo Milton , Veríssimo Ana Cristina , Ribeiro Laura
Published: October 2021

The integration of scientific research into medical curricula remains insufficient despite its advantages for medical students’ professional development and the advancement of medicine. This study aimed to evaluate the impact of first-year medical course attendance on medical students’ attitudes and perceptions towards scientific research and clinical practice, while also assessing the contribution of sociodemographic and academic factors. Two hundred and thirteen medical students self-administrated a questionnaire at the beginning and at the end of the first school year. Their responses were compared and two regression models were calculated to assess factors influencing students’ attitudes and perceptions. After freshman year, students displayed significantly lower positive attitudes towards science and research. Their motivation to perform research and to integrate it into the curriculum also decreased, while the importance attributed to research skills for clinical practice increased. Motivation to perform research and negative attitudes were positively and negatively associated with grade point average (GPA), respectively. Female students and those who attended public secondary schools attributed greater importance to communication skills. This study reinforces the need to early develop research skills and positive attitudes in medical students, motivating them to become physician-scientists. Additional follow-up studies may offer further contributions to the integration of research into medical curricula.
Numerical Investigation of Shallow Liquid Sloshing in a Baffled Tank and the Associated Damping Effect by BM-MPS Method
Authors: Wang Lizhu , Xu Min , Zhang Qian
Published: October 2021

Understanding the damping mechanism of baffles is helpful to make more reasonable use of them in suppressing liquid sloshing. In this study, the damping effect and mechanism of vertical baffles in shallow liquid sloshing under a rotational excitation are investigated by an improved particle method. By incorporation of a background mesh scheme and a modified pressure gradient model, the accuracy of impact pressure during sloshing is significantly enhanced. Combined with the advantages of the particle method, the present numerical method is a wonderful tool for the investigation of liquid sloshing issues. Through the analysis of impact pressure, the influences of baffle height and baffle position on the damping mechanism are discussed. The results show that the damping effect of vertical baffles increases with the increase of the elevation of baffle top and decreases with the increase of the elevation of the baffle bottom. Moreover, the resonance characteristics of sloshing are altered when static water is divided into two parts by the vertical baffle. The dominant damping mechanism of vertical baffles depends on the configurations.
Soil Quality Assessment Using Multivariate Approaches: A Case Study of the Dakhla Oasis Arid Lands
Authors: Selmy Salman A.H. , Abd Al-Aziz Salah H. Abd , Jiménez-Ballesta Raimundo , Jesús García-Navarro Francisco , Fadl Mohamed E.
Published: October 2021

A precise evaluation of soil quality (SQ) is important for sustainable land use planning. This study was conducted to assess soil quality using multivariate approaches. An assessment of SQ was carried out in an area of Dakhla Oasis using two methods of indicator selection, i.e., total data set (TDS) and minimum data set (MDS), and three soil quality indices (SQIs), i.e., additive quality index (AQI), weighted quality index (WQI), and Nemoro quality index (NQI). Fifty-five soil profiles were dug and samples were collected and analyzed. A total of 16 soil physicochemical parameters were selected for their sensitivity in SQ appraising to represent the TDS. The principal component analysis (PCA) was employed to establish the MDS. Statistical analyses were performed to test the accuracy and validation of each model, as well as to understand the relationship between the used methods and indices. The results of principal component analysis (PCA) showed that soil depth, gravel content, sand fraction, and exchangeable sodium percentage (ESP) were included in the MDS. High positive correlations (r ≥ 0.9) occurred between SQIs calculated using TDS and/or MDS under the three models. Moreover, the findings showed highly significant differences (p < 0.001) among SQIs within and between TDS and MDS. Approximately 80 to 85% of the total study area based on TDS, as well as 70 to 75%, according to MDS, were identified as suitable soils with slight limitations on soil quality grade (Q3, Q2, and Q1), while the remaining 20 to 30% had high to severe limitations (Q4 and Q5). The highest sensitivity (SI = 2.9) occurred by applying WQI using MDS and indicator weights based on the variance of PCA. Furthermore, the highest linear regression value (R2 = 0.88) between TDS and MDS was recorded using the same model. Because of its high sensitivity, such a model could be used for monitoring SQ changes caused by agricultural practices and environmental factors. The findings of this study have significant guiding implications and practical value in assessing the soil quality using TDS and MDS in arid areas critically and accurately.
The Structure and Functions of PRMT5 in Human Diseases
Authors: Motolani Aishat , Martin Matthew , Sun Mengyao , Lu Tao
Published: October 2021

Since the discovery of protein arginine methyltransferase 5 (PRMT5) and the resolution of its structure, an increasing number of papers have investigated and delineated the structural and functional role of PRMT5 in diseased conditions. PRMT5 is a type II arginine methyltransferase that catalyzes symmetric dimethylation marks on histones and non-histone proteins. From gene regulation to human development, PRMT5 is involved in many vital biological functions in humans. The role of PRMT5 in various cancers is particularly well-documented, and investigations into the development of better PRMT5 inhibitors to promote tumor regression are ongoing. Notably, emerging studies have demonstrated the pathological contribution of PRMT5 in the progression of inflammatory diseases, such as diabetes, cardiovascular diseases, and neurodegenerative disorders. However, more research in this direction is needed. Herein, we critically review the position of PRMT5 in current literature, including its structure, mechanism of action, regulation, physiological and pathological relevance, and therapeutic strategies.
Prevalence and Risk Factors for ESBL/AmpC-E. coli in Pre-Weaned Dairy Calves on Dairy Farms in Germany
Authors: Weber Laura Patricia , Dreyer Sylvia , Heppelmann Maike , Schaufler Katharina , Homeier-Bachmann Timo , Bachmann Lisa
Published: October 2021

The objectives of this study were to ascertain the fecal ESBL/AmpC-E. coli prevalence and to detect risk factors for their occurrence in young pre-weaned calves and their dams on large dairy farms in Germany. From 2018–2019 we investigated 2816 individual fecal samples from pre-weaned dairy calves and their dams, representing seventy-two farms (mean = 667 milking cows) from eight German federal states. To assess possible risk factors associated with ESBL/AmpC-E. coli prevalence in calves and dams, a questionnaire was performed, collecting management data. We observed an ESBL/AmpC-E. coli prevalence of 63.5% (95% CI: 57.4–69.5) among the sampled calves and 18.0% (95% CI: 12.5–23.5) among the dams. On all farms, at least one positive sample was obtained. To date, this is the highest ESBL/AmpC-E. coli prevalence observed in dairy herds in Europe. Feeding with waste milk was identified as a significant risk factor for a high prevalence of ESBL/AmpC-E. coli in calves. Many calves at large dairies in Germany are fed with waste milk due to the large amounts generated as a result of antibiotic dry-off routines and mastitis treatment with antibiotics. Other notable risk factors for high ESBL/AmpC-E. coli in calves were the general fitness/health of dams and calves, and the quality of farm hygiene. Taken together, these findings suggest that new or improved approaches to animal health management, for example, antibiotic dry cow management (selective dry cow therapy) and mastitis treatment (high self-recovery), as well as farm hygiene, should be researched and implemented.
Phyto-Extract-Mediated Synthesis of Silver Nanoparticles Using Aqueous Extract of Sanvitalia procumbens, and Characterization, Optimization and Photocatalytic Degradation of Azo Dyes Orange G and Direct Blue-15
Authors: Aslam Madeeha , Fozia Fozia , Gul Anadil , Ahmad Ijaz , Ullah Riaz , Bari Ahmed , Mothana Ramzi A. , Hussain Hidayat
Published: October 2021

Green synthesis of silver nanoparticles (AgNPs) employing an aqueous plant extract has emerged as a viable eco-friendly method. The aim of the study was to synthesize AgNPs by using plant extract of Sanvitalia procumbens (creeping zinnia) in which the phytochemicals present in plant extract act as a stabilizing and reducing agent. For the stability of the synthesized AgNPs, different parameters like AgNO3 concentration, volume ratios of AgNO3, temperature, pH, and contact time were studied. Further, AgNPs were characterized by UV–visible spectroscopy, FT-IR (Fourier Transform Infrared Spectroscopy), XRD (X-ray Diffraction), SEM (Scanning Electron Microscopy), and EDX (Energy Dispersive X-ray Spectrometer) analysis. FT-IR analysis showed that the plant extract contained essential functional groups like O–H stretching of carboxylic acid, N–H stretching of secondary amides, and C–N stretching of aromatic amines, and C–O indicates the vibration of alcohol, ester, and carboxylic acid that facilitated in the green synthesis of AgNPs. The crystalline nature of synthesized AgNPs was confirmed by XRD, while the elemental composition of AgNPs was detected by energy dispersive X-ray analysis (EDX). SEM studies showed the mean particle diameter of silver nanoparticles. The synthesized AgNPs were used for photocatalytic degradation of Orange G and Direct blue-15 (OG and DB-15), which were analyzed by UV-visible spectroscopy. Maximum degradation percentage of OG and DB-15 azo dyes was observed, without any significant silver leaching, thereby signifying notable photocatalytic properties of AgNPs.