bluuebunny/crossref_metadata_2025 huggingface dataset:
Finger-specific effects of age on tapping speed and motor fatigability
Authors: Heimhofer Caroline , Neumann Amira , Odermatt Ingrid , Bächinger Marc , Wenderoth Nicole
Published: September 2024

Introduction

Increased motor fatigability is a symptom of many neuromuscular and neurodegenerative disorders. However, it is difficult to pinpoint pathological motor fatigability, since the phenomena has not yet been fully characterized in the healthy population. In this study, we investigate how motor fatigability differs across age. Given that many disorders involve supraspinal components, we characterize motor fatigability with a paradigm that has previously been associated with supraspinal mechanisms. Finger tapping at maximal speed results in a rapid decrease in movement speed, which is a measure of motor fatigability.

Methods

We collected finger tapping data in a field experiment from the general population with a smartphone app, and we investigated age differences in maximal tapping speed, as well as the decrease in tapping speed for the index, middle, and little fingers.

Results

We found that the maximal tapping speed differed significantly between young (18–30 years, n = 194) and aged (50–70 years, n = 176), whereas the fatigability-induced relative decrease in movement speed did not differ between the age groups (average decrease: 17.0% ± 6.9% (young) vs. 16.5% ± 7.5% (aged) decrease). Furthermore, tapping speed and motor fatigability depended on which finger was used.

Discussion

These findings might relate to dexterity, with more dexterous movements being more resistant to fatigue. In this study, we provide a characterization of motor fatigability in the general population which can be used as a comparison for clinical populations in the future.
The differential effect of optogenetic serotonergic manipulation on sustained motor actions and waiting for future rewards in mice
Authors: Taira Masakazu , Miyazaki Kayoko W. , Miyazaki Katsuhiko , Chen Jianning , Okitsu-Sakurayama Shiho , Chaudhary Anupama , Nishio Mika , Miyake Tsukasa , Yamanaka Akihiro , Tanaka Kenji F. , Doya Kenji
Published: September 2024

Serotonin is an essential neuromodulator that affects behavioral and cognitive functions. Previous studies have shown that activation of serotonergic neurons in the dorsal raphe nucleus (DRN) promotes patience to wait for future rewards. However, it is still unclear whether serotonergic neurons also regulate persistence to act for future rewards. Here we used optogenetic activation and inhibition of DRN serotonergic neurons to examine their effects on sustained motor actions for future rewards. We trained mice to perform waiting and repeated lever-pressing tasks with variable reward delays and tested effects of optogenetic activation and inhibition of DRN serotonergic neurons on task performance. Interestingly, in the lever-pressing task, mice tolerated longer delays as they repeatedly pressed a lever than in the waiting task, suggesting that lever-pressing actions may not simply be costly, but may also be subjectively rewarding. Optogenetic activation of DRN serotonergic neurons prolonged waiting duration in the waiting task, consistent with previous studies. However, its effect on lever presses was nuanced, and was detected only by focusing on the period before premature reward check and by subtracting the trends within and across sessions using generalized linear model. While optogenetic inhibition decreased waiting, it did not affect lever pressing time or numbers. These results revealed that the necessity of motor actions may increase motivation for delayed rewards and that DRN serotonergic neurons more significantly promote waiting rather than persistent motor actions for future rewards.
SMLS-YOLO: an extremely lightweight pathological myopia instance segmentation method
Authors: Xie Hanfei , Yuan Baoxi , Hu Chengyu , Gao Yujie , Wang Feng , Wang Yuqian , Wang Chunlan , Chu Peng
Published: September 2024

Pathological myopia is a major cause of blindness among people under 50 years old and can result in severe vision loss in extreme cases. Currently, its detection primarily relies on manual methods, which are slow and heavily dependent on the expertise of physicians, making them impractical for large-scale screening. To tackle these challenges, we propose SMLS-YOLO, an instance segmentation method based on YOLOv8n-seg. Designed for efficiency in large-scale screenings, SMLS-YOLO employs an extremely lightweight model. First, StarNet is introduced as the backbone of SMLS-YOLO to extract image features. Subsequently, the StarBlock from StarNet is utilized to enhance the C2f, resulting in the creation of the C2f-Star feature extraction module. Furthermore, shared convolution and scale reduction strategies are employed to optimize the segmentation head for a more lightweight design. Lastly, the model incorporates the Multi-Head Self-Attention (MHSA) mechanism following the backbone to further refine the feature extraction process. Experimental results on the pathological myopia dataset demonstrate that SMLS-YOLO outperforms the baseline YOLOv8n-seg by reducing model parameters by 46.9%, increasing Box mAP@0.5 by 2.4%, and enhancing Mask mAP@0.5 by 4%. Furthermore, when compared to other advanced instance segmentation and semantic segmentation algorithms, SMLS-YOLO also maintains a leading position, suggesting that SMLS-YOLO has promising applications in the segmentation of pathological myopia images.
Dietary inflammatory index and all-cause mortality in adults with COPD: a prospective cohort study from the NHANES 1999–2018
Authors: Tian Tu-Lei , Zhi Tian-Yu , Xie Mei-Ling , Jiang Ya-Lin , Qu Xiang-Kun
Published: September 2024

Background

Chronic inflammation is closely linked to Chronic Obstructive Pulmonary Disease (COPD); however, the impact of the Dietaryq Inflammatory Index (DII) on mortality among COPD patients remains uncertain.

Objective

To assess the correlation between the DII and all-cause mortality in COPD patients using data from the National Health and Nutrition Examination Survey (NHANES).

Methods

We conducted a retrospective cohort study on 1,820 COPD patients from the NHANES dataset (1999-2018). The influence of DII on mortality was evaluated using multivariate Cox regression, smoothing spline fitting, and threshold effect analysis. Additionally, Kaplan-Meier survival analysis was performed to compare survival curves among different DII groups. Subgroup analyses and E-values identified sensitive cohorts and assessed unmeasured confounding.

Results

Over an average follow-up of 91 months, multivariate Cox regression models revealed a significant positive correlation between DII scores and mortality risk, with each unit increase in DII associated with a 10% higher risk of death (HR: 1.10, 95% CI: 1.03-1.16; P = 0.002). Among the DII tertiles, individuals in the second tertile (T2: 1.23-2.94) experienced a 67% increase in mortality risk compared to those in the lowest tertile (T1: -5.28-1.23) (HR: 1.67, 95% CI: 1.26-2.21; p < 0.001). The third tertile (T3) did not show a statistically significant increase in mortality risk (HR: 1.30, 95% CI: 0.98-1.72; p=0.074). A restricted cubic spline analysis indicated a significant nonlinear association between DII and all-cause mortality (p = 0.021). Threshold effect analysis further revealed that below a DII of 2.19, there was a significant increase in all-cause mortality risk (HR = 1.19, 95% CI: 1.07-1.33; p = 0.002), while at or above this threshold, the risk increase was not statistically significant (HR=0.89, 95% CI: 0.68-1.15; p = 0.380). Kaplan-Meier analysis revealed significant differences in survival curves among DII tertiles (p < 0.001), with the lowest DII tertile showing the highest survival probability. Both subgroup and sensitivity analyses confirmed the robustness of these findings.

Conclusion

DII is positively correlated with mortality risk in COPD patients, showing nonlinear characteristics and threshold effects, underscoring its prognostic value.
Association between serum iron levels and atherosclerotic cardiovascular diseases among American older adults: a cross-sectional study based on the National Health and Nutrition Examination Survey, 2009–2018
Authors: Yu Xiaochen , Li Min , Chen Bingxing , Qi Yuan , Guan Xiuru
Published: September 2024

Background

There is controversy regarding the relationship between serum iron levels and atherosclerotic cardiovascular disease (ASCVD).

Objective

To investigate the relationship between serum iron levels and ASCVD among older adults using data from the 2009–2018 National Health and Nutrition Examination Survey (NHANES).

Methods

We performed a cross-sectional analysis involving 8,682 participants aged 60 years and older, with complete data on serum iron levels and confirmed ASCVD status, sourced from the 2009–2018 National Health and Nutrition Examination Survey (NHANES). Multivariable logistic regression models were used to examine the association between serum iron levels and ASCVD. To assess the consistency of this association across different demographic groups, subgroup analyses, and interaction tests were performed.

Results

The group with the highest serum iron levels (fourth quartile, 100–369 μg/dL) exhibited several distinct characteristics: they were the youngest on average (69.57 ± 6.91 years), had the highest proportion of males (61.42%), and the highest hemoglobin levels (14.43 ± 1.33 g/dL). This group also showed the lowest iron supplement usage (19.71 ± 12.85 mg/30 days), white blood cell counts (6.73 ± 2.41 1,000 cells/μL), and serum creatinine levels (0.98 ± 0.45 mg/dL). Moreover, they had higher levels of education and income, a higher likelihood of being married, and a lower body mass index (BMI). Additionally, they had significantly lower rates of diabetes, hypertension, stroke, and heart attacks (all p < 0.05). After adjusting for potential confounders, a linear relationship between serum iron levels and ASCVD was initially observed (OR = 0.97; 95% CI, 0.95–0.99, p < 0.05). However, further analysis using a two-part logistic regression model with an inflection point at 131 μg/dL revealed more nuanced results. For serum iron levels below 131 μg/dL, each 10 μg/dL increase was associated with a 4% decrease in the odds of ASCVD (OR = 0.96; 95% CI, 0.93–0.98, p < 0.001). Conversely, for serum iron levels above 131 μg/dL, each 10 μg/dL increase corresponded to a 1% increase in the odds of ASCVD, though this finding was not statistically significant (OR = 1.01; 95% CI, 0.98–1.08, p > 0.05).

Conclusion

In the US elderly population, serum iron levels are negatively associated with ASCVD, particularly when serum iron levels are below 131 μg/dL.
Risk–benefit assessment of foods and its role to inform policy decisions: outcome of an international workshop
Authors: Matteu Monteiro Constanza De , Membré Jeanne-Marie , Poulsen Morten , Thomsen Sofie Theresa , Pires Sara Monteiro
Published: September 2024

Policy decisions in public health require consideration and evaluation of trade-offs for which transparency and science-based evidence is needed. Improvement of decision-support tools is essential to help guide food policy decisions that promote healthy diets and meet the challenges of food systems without compromising food security, food safety, and sovereignty. Risk–benefit assessment of foods (RBA) is an established methodological approach designed to inform policy decisions within the area of nutrition and food safety. Despite methodological developments, translation of RBA findings into policies is still limited. In this context, a stakeholder workshop held in May 2023 gathered RBA experts and food regulators from Europe to identify the challenges, obstacles and opportunities in using evidence generated through RBAs to inform food policy decisions. A structured process was implemented to collect their views through online surveys, breakout groups, and plenary discussions. As a secondary objective, food regulators’ views on other approaches for holistic risk assessment fit for food systems analysis were also explored. This paper summarizes the main findings of the workshop and discusses policy implications and future perspectives to improve the area of RBA and its role in food policymaking.
Association between dopamine genes, adiposity, food addiction, and eating behavior in Chilean adult
Authors: Luengo Nicole , Goldfield Gary S. , Obregón Ana M.
Published: September 2024

Background

A frequent consumption of high sugar/fat foods can affect dopamine signaling in the brain and cause sustained stimulation of the reward system. It has been hypothesized that a hypodopaminergic trait results in an individual overeating in order to increase brain DA. Genetic variants in this route have been connected with addiction and eating behaviors. Most studies focus on a specific SNP, and few studies have used multilocus genetic scores, which quantify genetic risk on a continuum.

Aim

To assess the relationship between multilocus genetic scores based on multiple gene variants in the dopaminergic pathway and measurements of anthropometry, eating behavior, food reinforcement, and food addiction (FA) in Chilean adults.

Methods

We recruited 221 Chilean adults for a cross-sectional study. A standard anthropometric measurement procedure was followed and eating behavior was examined using the Three Factor Eating questionnaire (TFEQ), Food Reinforcement Value Questionnaire (FRVQ), Yale Food Addiction Scale (YFAS) and 24-h diet recall. Multilocus genetic scores were calculated using TaqMan assays (rs1800497-rs1799732-rs6277-rs4680).

Results

No differences were found in the entire sample for anthropometric measurements, by MLGS. We found that participants with a score ≥ 2.0 in the MLGS showed higher food choices on the RVFQ and lower energy intake in protein, lipids, SAFA, MUFA, PUFA, dietary cholesterol, omega-3 and Omega-6 fatty acids in the 24-h recall (p < 0.05). Stratified by nutritional condition, the group with obesity had inferior scores on cognitive restriction, greater scores on uncontrolled eating, emotional eating, and responding to palatable food in the RVFQ. Also, in subjects with obesity, there was more food addiction in the group scoring “MLGS ≥2.0 or low dopamine signaling” (53%), compared to the group scored “MLGS <2.0 or high dopamine signaling” (23%) (p-value; 0.05). Emotional Eating scores correlated positively with MLGS in subjects with obesity.

Conclusion

In adults with obesity, the MLGS of the dopamine pathway, reflecting hypodopaminergic signaling, was associated with greater scores on food addiction and altered eating behavior traits.
Global burden and health inequality of nutritional deficiencies from 1990 to 2019
Authors: Yu Yang , Li Hui , Hu Nan-xi , Wu Xiao-hua , Huang Xin-yi , Lin Hong-tao , Yu Kai-li , Li Jin-luan
Published: September 2024

Introduction

Nutritional deficiencies (NDs) manifest in various forms and are widespread globally. However, a systematic evaluation of the epidemiology of NDs across all causes and age groups in different countries and regions has not been conducted.

Materials and methods

This study aimed to utilize data from the 2019 Global Burden of Disease (GBD) study to assess the burden and trends of NDs, including their incidence, prevalence, mortality, and disability-adjusted life years (DALYs). Additionally, the study evaluated health inequalities at global, regional, and national levels from 1990 to 2019.

Result

In 2019, the age-standardized incidence rate of NDs was 2,207.71 per 100,000 individuals (95% UI 1,863.04–2,604.67), and the age-standardized DALYs (ASR-DALYs) was 680.12 per 100,000 individuals (95% UI 507.21–894.89). Among the causes of NDs, dietary iron deficiency had the highest ASR-DALYs and exhibited minimal variation. Children under the age of 5 years faced the greatest risk of NDs. Sex disparity was evident, with males having lower rates than females. Although the gap in the burden of NDs between regions classified as poor and wealthy decreased, disparities persist.

Conclusion

These findings provide critical insights for the development of global health strategies aimed at mitigating NDs and may guide policymakers in implementing effective and economically viable interventions.
Association between dynapenic obesity phenotypes and physical performance in middle-age and older women living in community
Authors: Arteaga-Pazmiño Cecilia , Fonseca-Pérez Diana , Balladares Mazzini Manuel , Galvez-Celi Javier , Emén Sánchez Janet , Álvarez-Córdova Ludwig
Published: September 2024

Background

Dynapenic obesity (DO) is the coexistence of excess adipose tissue/body weight and low muscle strength. This condition is associated with an increased risk of suffering from various chronic diseases and physical deterioration in older people.

Aim

To analyze the association between DO phenotypes and physical performance in middle-aged women living in the community.

Methods

This cross-sectional study was conducted on middle-aged and older women (≥50 years) residing in Guayaquil, Ecuador. Dynapenia was diagnosticated by a handgrip strength (HGS) < 16 kg; obesity was determined based on body mass index (BMI) ≥ 30 kg/m2. Participants were categorized into four groups based on their dynapenia and obesity status: non-dynapenic/non-obesity (ND/NO), obesity/non-dynapenic (O/ND), dynapenic/non-obesity (D/NO) and dynapenic/obesity (D/O). Physical performance was assessed by the Short Physical Performance Battery (SPPB).

Results

A total of 171 women were assessed. The median (IQR) age of the sample was 72.0 (17.0) years. Obesity and dynapenia were 35% (n = 60) and 57.8% (n = 99) of the participants, respectively. The prevalence of ND/NO was 25.1% (n = 43), O/ND 17% (n = 29), D/NO 39.8% (n = 68) and DO 18.1% (n = 31). The mean SPPB total score was 6.5 ± 3.2. Participants of D/NO and DO groups presented significantly lower mean SPPB scores (p < 0.001) compared to those of NO/ND and O/ND groups.

Conclusion

Women with DO and D/NO exhibited significantly lower SPPB scores, indicating poorer physical performance. These findings emphasize the importance of incorporating a comprehensive assessment of muscle strength and obesity in middle-aged and older women.
ASPYRE-Lung: validation of a simple, fast, robust and novel method for multi-variant genomic analysis of actionable NSCLC variants in FFPE tissue
Authors: Evans Ryan T. , Gillon-Zhang Elizabeth , Brown Julia N. , Knudsen Katherine E. , King Candace , Green Amanda S. , Silva Ana-Luisa , Mordaka Justyna M. , Palmer Rebecca N. , Tomassini Alessandro , Collazos Alejandra , Xyrafaki Christina , Turner Iyelola , Ho Chau Ha , Nugent Dilyara , Jose Jinsy , Andreazza Simonetta , Potts Nicola D. , von Bargen Kristine , Gray Eleanor R. , Stolarek-Januszkiewicz Magdalena , Cooke Aishling , Reddi Honey V. , Balmforth Barnaby W. , Osborne Robert J.
Published: September 2024

Introduction

Genomic variant testing of tumors is a critical gateway for patients to access the full potential of personalized oncology therapeutics. Current methods such as next-generation sequencing are costly and challenging to interpret, while PCR assays are limited in the number of variants they can cover. We developed ASPYRE® (Allele-Specific PYrophosphorolysis REaction) technology to address the urgent need for rapid, accessible and affordable diagnostics informing actionable genomic target variants of a given cancer. The targeted ASPYRE-Lung panel for non-small cell carcinoma covers 114 variants in 11 genes (ALK, BRAF, EGFR, ERBB2, KRAS, RET, ROS1, MET & NTRK1/2/3) to robustly inform clinical management. The assay detects single nucleotide variants, insertions, deletions, and gene fusions from tissue-derived DNA and RNA simultaneously.

Methods

We tested the limit of detection, specificity, analytical accuracy and analytical precision of ASPYRE-Lung using FFPE lung tissue samples from patients with non-small cell lung carcinoma, variant-negative FFPE tissue from healthy donors, and FFPE-based contrived samples with controllable variant allele fractions.

Results

The sensitivity of ASPYRE-Lung was determined to be ≤ 3% variant allele fraction for single nucleotide variants and insertions or deletions, 100 copies for fusions, and 200 copies for MET exon 14 skipping. The specificity was 100% with no false positive results. The analytical accuracy test yielded no discordant calls between ASPYRE-Lung and expected results for clinical samples (via orthogonal testing) or contrived samples, and results were replicable across operators, reagent lots, runs, and real-time PCR instruments with a high degree of precision.

Conclusions

The technology is simple and fast, requiring only four reagent transfer steps using standard laboratory equipment (PCR and qPCR instruments) with analysis via a cloud-based algorithm. The ASPYRE-Lung assay has the potential to be transformative in facilitating access to rapid, actionable molecular profiling of tissue for patients with non-small cell carcinoma.
Transumbilical single-site laparoscopic treatment of primary splenic cyst in child: a rare case report and review of literature
Authors: Kong Meng , Chen Shuai , Bai Yuexia , Yan Yuxi , Yi Minggang , Wang Meiyun , Liu Hongzhen , Jia Jinhua , Liu Chuanyang , Zhang Shisong
Published: September 2024

Background

Splenic cysts are relatively rare benign tumors that are asymptomatic in most patients and are usually discovered incidentally by imaging. In our case, we report a splenic cyst in a child who underwent laparoscopic partial splenectomy.

Case description

A 13-year-old boy was admitted to the hospital after an incidental finding of a splenic cyst on an abdominal ultrasound conducted 4 days prior. He was asymptomatic before admission. Upon admission, abdominal ultrasound and CT revealed a cystic lesion in the spleen, highly suspicious for a splenic cyst. Then, we used transumbilical single-site laparoscopic exploration and found a cyst measuring approximately 12 cm × 11 cm × 10 cm at the upper pole of the spleen, so we performed a partial splenectomy and diagnosed a primary epithelioid splenic cyst via postoperative pathology.

Conclusions

Splenic cysts in children are very rare and can be treated conservatively in asymptomatic patients with a diameter of less than 5 cm, while surgery is required in symptomatic patients or those with a diameter greater than or equal to 5 cm. Transumbilical single-site laparoscopic partial splenectomy is a minimally invasive and effective treatment, especially for children.
Muscarinic receptor drug trihexyphenidyl can alter growth of mesenchymal glioblastoma in vivo
Authors: Du Renfei , Sanin Ahmed Y. , Shi Wenjie , Huang Bing , Nickel Ann-Christin , Vargas-Toscano Andres , Huo Shuran , Nickl-Jockschat Thomas , Dumitru Claudia A. , Hu Wei , Duan Siyu , Sandalcioglu I. Erol , Croner Roland S. , Alcaniz Joshua , Walther Wolfgang , Berndt Carsten , Kahlert Ulf D.
Published: September 2024

Glioblastoma (GBM) is the most commonly occurring and most aggressive primary brain tumor. Transcriptomics-based tumor subtype classification has established the mesenchymal lineage of GBM (MES-GBM) as cancers with particular aggressive behavior and high levels of therapy resistance. Previously it was show that Trihexyphenidyl (THP), a market approved M1 muscarinic receptor-targeting oral drug can suppress proliferation and survival of GBM stem cells from the classical transcriptomic subtype. In a series of in vitro experiments, this study confirms the therapeutic potential of THP, by effectively suppressing the growth, proliferation and survival of MES-GBM cells with limited effects on non-tumor cells. Transcriptomic profiling of treated cancer cells identified genes and associated metabolic signaling pathways as possible underlying molecular mechanisms responsible for THP-induced effects. In vivo trials of THP in immunocompromised mice carry orthotopic MES-GBMs showed moderate response to the drug. This study further highlights the potential of THP repurposing as an anti-cancer treatment regimen but mode of action and d optimal treatment procedures for in vivo regimens need to be investigated further.
Neuroprotective effects of Gastrodia elata Blume on promoting M2 microglial polarization by inhibiting JNK/TLR4/T3JAM/NF-κB signaling after transient ischemic stroke in rats
Authors: Huang Shang-Chih , Huang Hui-Chi , Liao Wen-Ling , Kao Shung-Te , Cheng Chin-Yi
Published: September 2024

Background

Gastrodia elata Blume, also called Tian Ma (TM), has been used to treat stroke for centuries. However, its effects on inflammation in acute cerebral ischemic injury and underlying mechanisms involved in microglial polarization remain unknown. The present study explored the effects of the TM extract on the modulation of microglial M1/M2 polarization 2 days after transient cerebral ischemia.

Methods

Male Sprague Dawley rats were intracerebroventricularly administered with 1% dimethyl sulfoxide 25 min before cerebral ischemia and subsequently intraperitoneally administered 0.25 g/kg (DO + TM-0.25 g), 0.5 g/kg (DO + TM-0.5 g), or 1 g/kg (DO + TM-1 g) of the TM extract after cerebral ischemia onset.

Results

DO + TM-0.5 g and DO + TM-1 g treatments downregulated the following: phospho-c-Jun N-terminal kinase (p-JNK)/JNK, tumor necrosis factor (TNF) receptor-associated factor 3 (TRAF3), TRAF3-interacting JNK-activating modulator (T3JAM), p-nuclear factor-kappa B p65 (p-NF-κB p65)/NF-κB p65, ionized calcium-binding adapter molecule 1 (Iba1), CD86, TNF-α, interleukin (IL)-1β, and IL-6 expression and toll-like receptor 4 (TLR4)/Iba1, CD86/Iba1, and p-NF-κB p65/Iba1 coexpression. These treatments also upregulated IL-10, nerve growth factor, and vascular endothelial growth factor A expression and YM-1/2/Iba1 and IL-10/neuronal nuclei coexpression in the cortical ischemic rim. The JNK inhibitor SP600125 exerted similar treatment effects as the DO + TM-0.5 g and DO + TM-1 g treatments.

Conclusion

DO + TM-0.5 g and DO + TM-1 g/kg treatments attenuate cerebral infarction by inhibiting JNK-mediated signaling. TM likely exerts the neuroprotective effects of promoting M1 to M2 microglial polarization by inhibiting JNK/TLR4/T3JAM/NF-κB-mediated signaling in the cortical ischemic rim 2 days after transient cerebral ischemia.
Malva sylvestris leaf powder as a feed additive affects the performance, carcass traits, meat quality attributes, serum antioxidants, stress physiology, intestinal bacterial counts, and gut morphology of broiler chicken
Authors: Banday Mohammad T. , Wani Manzoor A. , Alqhtani Haifa A. , Bin-Jumah May , Rudayni Hassan A. , Allam Ahmed A. , Algopishi Uthman Balgith , Adil Sheikh
Published: September 2024

This study investigated the effect of supplementation of Malva sylvestris leaf powder (MSLP) on the production performance of broiler chicken. Ven Cobb broiler chicks (240 day-old male chicks) were distributed randomly into four treatments, each replicated four times, with 15 birds per replicate. The diets formulated were T1 (control) given basal diet only, T2 (basal diet +1.0% MSLP), T3 (basal diet +1.5% MSLP), and T4 (basal diet +2.0% MSLP). The highest improvement of 3.83% in the average daily gain (ADG) was recorded in the T3 group fed 1.5% Malva powder in the diet compared to the control (P = 0.009). The average daily feed intake (ADFI) tended to decrease with an increase in the dose of MSLP in the diet, with the lowest feed intake in the T4 group fed 2% MSLP. During the overall period (7–42 days), the feed/gain (F/G) ratio reduced significantly (P = 0.048) in the T3 and T4 groups compared to the control. The dressed and breast meat yield was found to be significantly (P < 0.05) higher in the T3 group, with no significant change (P > 0.05) in the thigh yield. The changes in the pH and water-holding capacity (WHC) of breast meat were found to be non-significant (p > 0.05) between the control and various other treatments. Thiobarbituric acid-reactive substances (TBARS) were significantly (P < 0.05) decreased in the T3 and T4 groups. There was no negative effect of including MSLP in the diet on the color coordinates of breast meat among different treatments. Compared to the control, the serum immunoglobulin values increased significantly (P < 0.05) in the T3 and T4 groups. Superoxide dismutase (SOD) showed no difference between various treatments; however, malondialdehyde (MDA) decreased significantly (P < 0.05) according to dietary treatments. Serum cortisol increased significantly (P < 0.05) in the T4 group compared to other treatments. The inclusion of Malva powder in the diet at the 2% level significantly (P < 0.05) decreased the coliform count compared to the control birds. Supplementation with Malva powder resulted in a significant (P < 0.05) increase in the villus height-to-crypt depth (VH:CD) ratio of broiler birds in the T3 and T4 groups. In conclusion, MSLP supplementation at 1.5% and 2% resulted in improved production performance of broiler chicken.
Bacillus velezensis GH1-13 enhances drought tolerance in rice by reducing the accumulation of reactive oxygen species
Authors: Park Dongryeol , Jang Jinwoo , Seo Deok Hyun , Kim Yangseon , Jang Geupil
Published: September 2024

Plant growth-promoting rhizobacteria colonize the rhizosphere through dynamic and intricate interactions with plants, thereby providing various benefits and contributing to plant growth. Moreover, increasing evidence suggests that plant growth-promoting rhizobacteria affect plant tolerance to abiotic stress, but the underlying molecular mechanisms remain largely unknown. In this study, we investigated the effect of Bacillus velezensis strain GH1-13 on drought stress tolerance in rice. Phenotypical analysis, including the measurement of chlorophyll content and survival rate, showed that B. velezensis GH1-13 enhances rice tolerance to drought stress. Additionally, visualizing ROS levels and quantifying the expression of ROS-scavenging genes revealed that GH1-13 treatment reduces ROS accumulation under drought stress by activating the expression of antioxidant genes. Furthermore, the GH1-13 treatment stimulated the jasmonic acid response, which is a key phytohormone that mediates plant stress tolerance. Together with the result that jasmonic acid treatment promotes the expression of antioxidant genes, these findings indicate that B. velezensis GH1-13 improves drought tolerance in rice by reducing ROS accumulation and suggest that activation of the jasmonic acid response is deeply involved in this process.
The effects of applying cellulase and laccase on fermentation quality and microbial community in mixed silage containing corn stover and wet brewer’s grains
Authors: Li Li , Xie Xiangxue , Zhao Guoqiang , He Jiajun , Zhang Yongliang
Published: September 2024

Objective

The purpose of this experiment was to explore the effect of adding cellulase and laccase on fermentation quality and microbial community in mixed silage of corn stover and wet brewer’s grains. Try to a new approach for the proper preservation and utilization of the agro-industrial by-products (corn stover and wet brewer’s grains).

Methods

The experiment was divided into four groups: CK (control), C (cellulase, 120 U/g fresh matter [FM]), L (laccase, 50 U/g FM), CL (cellulase 120 U/g FW and laccase 50 U/g FM), and the chemical composition, fermentation quality, microbial population and microbial community in mixed silage of corn stover and wet brewer’s grains after 30 day’s fermentation were determined.

Results

Compared to control, the addition of cellulase significantly increased crude protein (CP), water-soluble carbohydrate (WSC), lactic acid bacteria (LAB) counts, while significantly decreased the neutral detergent fiber (NDF), acid detergent fiber (ADF) content (P < 0.05). Adding laccase significantly decreased the acid detergent lignin (ADL) content (P < 0.05). Combined application of cellulase and laccase significantly increased the CP, WSC content and LAB counts, while significantly decreased pH value, NDF, ADF and ADL content (P < 0.05), thereby improving fermentation quality. In addition, the application of cellulase and laccase increased the abundance of Firmicutes and LAB genera, and decreased microbial diversity level of the sample.

Conclusion

The combined application of cellulase and laccase further improved fermentation quality and microbial community in mixed silage of corn stover and wet brewer’s grains.
An open-access database of video stimuli for action observation research in neuroimaging settings: psychometric evaluation and motion characterization
Authors: Georgiev Christian , Legrand Thomas , Mongold Scott J. , Fiedler-Valenta Manoa , Guittard Frédéric , Bourguignon Mathieu
Published: September 2024

Video presentation has become ubiquitous in paradigms investigating the neural and behavioral responses to observed actions. In spite of the great interest in uncovering the processing of observed bodily movements and actions in neuroscience and cognitive science, at present, no standardized set of video stimuli for action observation research in neuroimaging settings exists. To facilitate future action observation research, we developed an open-access database of 135 high-definition videos of a male actor performing object-oriented actions. Actions from 3 categories: kinematically natural and goal-intact (Normal), kinematically unnatural and goal-intact (How), or kinematically natural and goal-violating (What), directed toward 15 different objects were filmed from 3 angles. Psychometric evaluation of the database revealed high video recognition accuracy (Mean accuracy = 88.61 %) and substantial inter-rater agreement (Fleiss' Kappa = 0.702), establishing excellent validity and reliability. Videos' exact timing of motion onset was identified using a custom motion detection frame-differencing procedure. Based on its outcome, the videos were edited to assure that motion begins at the second frame of each video. The videos' timing of category recognition was also identified using a novel behavioral up-down staircase procedure. The identified timings can be incorporated in future experimental designs to counteract jittered stimulus onsets, thus vastly improving the sensitivity of neuroimaging experiments. All videos, their psychometric evaluations, and the timing of their frame of category recognition, as well as our custom programs for performing these evaluations on our, or on other similar video databases, are available at the Open Science Framework (https://osf.io/zexc4/).
How depression and ADHD relate to exercise addiction: a cross-sectional study among frequent exercisers
Authors: Baltes-Flueckiger Lavinia , Wagner Aline , Sattler Isabel , Meyer Maximilian , Tschopp Amos , Walter Marc , Colledge Flora
Published: September 2024

Background

To date, there are no official diagnostic criteria for the frequently reported phenomenon of exercise addiction. Therefore, the aim of the present study was to investigate how mental disorders, specifically depression and attention-deficit hyperactivity disorder (ADHD), are related to exercise addiction (EA).

Methods

A total of 173 participants aged between 18 and 70 years, who reported exercising more than 10 h a week and continued to exercise despite injury or illness, answered questionnaires including the Exercise Dependence Scale, the Beck Depression Inventory, and the Homburger ADHD scale for adults. Multiple linear regression analyses were performed adjusting for relevant confounders (age, gender) and stepwise regression was used to identify which of the two mental disorders is the more influential predictor of EA.

Results

Pearson correlation analysis showed that depressive symptoms [r (171) = 0.422, p < 0.00] and ADHD symptoms [r (171) = 0.308, p < 0.001] were positively correlated with EA symptoms. The relation between depressive symptoms and EA remained after adjusting for confounders in the regression model (B = 20.531; t(170) = 5.950; 95% CI [13.719, 27.343]; p < 0.001). Similarly, the positive link between ADHD symptoms and EA persisted after controlling for confounders (B = 15.507; t(170) = 3.771; 95% CI [7.389, 23.625]; p < 0.001). Additionally, a stepwise regression model identified that depressive symptoms are a stronger predictor for EA than ADHD symptoms.

Conclusion

Depressive symptoms seem to be a stronger predictor for EA compared to ADHD symptoms in frequent exercisers. Although individuals with ADHD May exercise extensively, they might be less at risk for EA than individuals with depression. These results contribute to the complex characterization of the psychiatric profile of individuals with exercise addiction, and underline the need for further research elucidating the interplay between mental disorders and EA.
Effect of the COVID-19 pandemic on infants’ development: analyzing the results of developmental assessments at ages 10–11 and 18–24 months
Authors: Otani Takashi , Kato Masaharu , Haraguchi Hisami , Goma Hideyo
Published: September 2024

The purpose of this study was to determine the effect of the COVID-19 pandemic on infant development. The study investigated the development of infants at 10–11 months of age between 2020 and 2023 by using the Kyoto Scale of Psychological Development-2020 (KSPD2020), an individualized developmental scale, and the Kinder Infant Developmental Scale (KIDS), a developmental questionnaire. We compared the results of the KSPD2020 with those of a pre-pandemic developmental research and compared the developmental age (DA) of KIDS with children’s chronological age (CA). Moreover, the same developmental research was conducted again on the same children at 18–24 months of age. DA for receptive language and expressive language was lower in the KIDS compared to CA in the investigation at 10–11 months. However, in the investigation at 18–24 months, there were no areas where KIDS’ DA was lower than CA, and DA in the areas of manipulation, receptive language, social relationship with adults, discipline, and eating was higher than CA. On the other hand, using the KSPD2020, there were no differences when compared to pre-pandemic data in the investigation at 10–11 months. Furthermore, the investigation at 18–24 months showed that developmental quotient (DQ) was lower in the Language-Social (L-S) areas than in the investigation at 10–11 months. The lower DQ of L-S in this study was also evident in comparison to the 18–24 months pre-pandemic data. These results suggested that to investigate the medium- and long-term effects of the COVID-19 pandemic on children’s development, it is necessary to use not only parent-filled questionnaires but also individualized developmental scales. In addition, the finding that results may differ depending on the method of developmental assessment is considered important not only for developmental researchers but also for professionals involved in supporting children’s development.
Ethical implications in using robots among older adults living with dementia
Authors: Deusdad Blanca
Published: September 2024

The aging of the world’s population due to accelerating demographic shift on all continents is causing increasing pressure worldwide, giving rise to a “crisis of care” or “care wave.” The increase in longevity is resulting in an increase in chronic diseases (such as dementia), an increase in care needs to perform the activities of daily living, and situations of isolation and profound loneliness among older adults. These circumstances are opening the debate on the need to use technology, such as robots, to improve the wellbeing of older adults and their caregivers. The aim of this paper is to address the ethical questions in using social and companion robots for people with dementia, such as concerning consent, the replacement of human care, the potential for increased dependency, and the burden on caregivers. Involving older adults and other stakeholders offers the potential to pursue robotics to support older people while also ensuring a strong ethical commitment. The study is a review of high-impact articles on the topic of the use of social and companion robots with older people with dementia.
Exploring cariprazine as a treatment option for varied depression symptom clusters
Authors: Pejušković Bojana , Munjiza Jovanović Ana , Pešić Danilo
Published: September 2024

Major depressive disorder (MDD) is among the most prevalent psychiatric conditions and a leading cause of disability worldwide. MDD presents a diverse range of symptoms that significantly impact personal, societal, and economic dimensions. Despite the availability of numerous antidepressant treatments (ADTs) targeting different molecular mechanisms, a substantial proportion of patients experience inadequate response, presenting a considerable challenge in MDD management. As a result, adjunctive strategies, particularly involving atypical antipsychotics, are often employed to enhance treatment efficacy. Cariprazine, a D2/D3 partial agonist, is distinguished from other atypical antipsychotics by its selective action on the D3 receptor and its modulation of 5-HT1A, 5-HT2A, and alpha 1B receptors. This distinctive pharmacological profile warrants investigation into its potential effectiveness and tolerability across various symptom domains of MDD, including pleasure, interest, and motivation; mood and suicidality; sleep and appetite; fatigue; psychomotor activity and anxiety; and cognitive function. Preliminary evidence from animal studies and clinical trials suggests that cariprazine may improve motivation, anhedonia, and cognitive function symptoms. Cariprazine shows promise in alleviating mood-related symptoms, though its impact on anxiety and its effects on agitation and psychomotor retardation remains uncertain. Cariprazine may be particularly beneficial for patients with MDD exhibiting anhedonia, cognitive deficits, and possibly fatigue and hypersomnia. Evaluating cariprazine’s efficacy across these symptom domains could reveal patterns that support more personalized treatment approaches for depression. Further research is essential to elucidate the role of cariprazine as an adjunctive therapy for adults with major depressive disorder who have an inadequate response to antidepressant monotherapy.
Economic evaluation of acupuncture in treating patients with pain and mental health concerns: the results of the Alberta Complementary Health Integration Project
Authors: Lu Mingshan , Sharmin Sumaiya , Tao Yong , Xia Xin , Yang Gongliang , Cong Yingying , Yang Guanhu , Razavilar Negar , Aziz Riffat , Jiang Jing , Xiao Yun , Peng Laura , Xu Bentong
Published: September 2024

Background

The COVID-19 pandemic and its economic impact have heightened the risk of mental health and pain-related issues. The integration of acupuncture with conventional medicine shows promise in improving treatment outcomes for these conditions. The Alberta Complementary Health Integration Project (ABCHIP) aimed to provide acupuncture to youth (aged 24 and under) and seniors (aged 55 and above) experiencing chronic pain, pain management issues, mental health issues, and/or related conditions. The program aimed to promote integrative care, assess the effectiveness and cost-effectiveness of these therapies, and deliver patient-centered care.

Design

ABCHIP provided acupuncture to address pain, mental health, and addiction issues at no cost to two vulnerable populations in Alberta: youth and the older adult. A total of 606 patients aged 14–65 received 5,424 acupuncture treatments. Outcome measures included pain interference, pain severity, sleep quality, depression, anxiety, fatigue, anger, and quality of life. Short-term outcomes were assessed through questionnaires completed at the beginning and completion of the treatments, while long-term benefits were estimated using these outcome indicators and existing literature on the economic cost of illnesses.

Result

The cost-effectiveness analysis revealed the following ratios per Quality-Adjusted Life Year (QALY): CND12,171 for the overall sample, CND10,766 for patients with pain, CND9,331 for individuals with depression, and CND9,030 for those with anxiety. The cost–benefit analysis demonstrated annual cost savings ranging from CND1,487 to CND5,255, with an average of CND3,371.

Conclusion

The study findings indicate that ABCHIP’s treatment for pain, depression, anxiety, and sleep issues is cost-effective, leading to substantial cost savings and improved quality of life for patients. The program’s cost per Quality-Adjusted Life Year (QALY) is significantly lower than benchmarks used in other countries, demonstrating high cost-effectiveness and value. Patients receiving 12 treatments experienced significant improvements across all measures, with estimated economic benefits surpassing treatment costs. In summary, ABCHIP offers a cost-effective and economically efficient therapy choice for individuals dealing with pain and mental health issues.
Radon exposure and potential health effects other than lung cancer: a systematic review and meta-analysis
Authors: Henyoh Afi Mawulawoe Sylvie , Laurent Olivier , Mandin Corinne , Clero Enora
Published: September 2024

Context and objective

To date, lung cancer is the only well-established health effect associated with radon exposure in humans. To summarize available evidence on other potential health effects of radon exposure, we performed a comprehensive qualitative and quantitative synthesis of the available literature on radon exposure and health effects other than lung cancer, in both occupational and general populations.

Method

Eligible studies published from January 1990 to March 2023, in English and French languages, were identified in PubMed, ScienceDirect, Scopus, ScieLo and HAL. In the meta-analysis, we estimated average weighted standardized incidence ratios (metaSIR), standardized mortality ratios (metaSMR), and risk ratio (metaRR) per 100 unit (Bq/m3 or Working level Month) increase in radon exposure concentration by combining estimates from the eligible studies using the random-effect inverse variance method. DerSimonian & Laird estimator was used to estimate the between-study variance. For each health outcome, analyses were performed separately for mine workers, children, and adults in the general population.

Results

A total of 129 studies were included in the systematic review and 40 distinct studies in the meta-analysis. For most of these health outcomes, the results of the meta-analyses showed no statistically significant association, and heterogeneity was only present among occupational studies, especially between those included in the metaSIR or metaSMR analyses. However, the estimated exposure-risk associations were positive and close to the statistical significance threshold for: lymphohematological cancer incidence in children (metaRR = 1.01; 95%CI: 1.00–1.03; p = 0.08); malignant melanoma mortality among adults in the general population (metaRR = 1.10; 95%CI: 0.99–1.21; p = 0.07); liver cancer mortality among mine workers (metaRR = 1.04; 95%CI: 1.00–1.10; p = 0.06); intestine and rectal cancer mortality combined among mine workers (metaRR = 1.02; 95%CI: 1.00–1.04; p = 0.06).

Conclusion

Although none of the exposure-risk associations estimated in the meta-analyses reached statistical significance, the hypothesis that radon may have other health effects apart from lung cancer could not be ruled-out and call for additional research. Larger and well-designed studies are needed to further investigate this question.

Systematic review registration

https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42023474542, ID: CRD42023474542.