bluuebunny/crossref_metadata_2025 huggingface dataset:
Self Directed Learning Scale at University Context
Authors: Masrul Masrul , Yuliani Sri
Published: April 2024

This research was carried out to investigate a relationship between self-directedness, self-involvement, expressive language, self-assessment, language varieties, metacognitive skills, editing and problem solving, text processing, knowledge rehearsal, idea planning, goal oriented monitoring and evaluating, peer learning, feedback handling, interest enhancement, motivational self-talk, and emotional control and  the main and forming factors of self-directedness, self-involvement, expressive language, self-assessment, language varieties, metacognitive skills, editing and problem solving, text processing, knowledge rehearsal, idea planning, goal oriented monitoring and evaluating, peer learning, feedback handling, interest enhancing, motivational self-talk, and emotional control of self directed learning. A convenience sample approach was used to recruit English majors from a range of State University of Yogyakarta. All participants were recruited intentionally, and that they were informed that they might leave the study at any moment either during collecting data. The participant criteria include second-year English major students from various State Universities of Yogyakarta. As the result of pearson correlation in this research, it was found that there was a substantial relationship between the variables examined in this research. Moreover, as the result of factor analysis in this research was found that from the 11 variables included in the research namely self involvement, expressive language, self assessment, language varieties, metacognitive skills, editing and problem solving, Cognition, Metacognition, Social Behavior, and Motivational Regulation, they can be grouped into two main factors.  The implications of these findings lie in a deeper understanding of the underlying conceptual structure of self-directed learning in a university context. With the identification of the key factors emerging from these variables, a more focused approach can be developed to enhance students' self-directed learning. This can also aid in the development of more precise and measurable assessment instruments to gauge progress in crucial aspects of self-directed learning. Thus, the implications of these findings can enrich teaching and learning practices in higher education settings.
Message from the Dean
Authors: Bertolami Charles
Published: April 2024

The Dean congratulates the Academy of Distinguished Educators on the 12th anniversary of its founding and for promoting excellence in educating tomorrow's health care providers across disciplines, innovation, experimentation, and evaluation of methods used for instruction and assessment and encouraging approaches that are reasonable and evidence-based.
The Dental Therapist: A Century of Controversy
Authors: Spielman Andrew
Published: April 2024

"Dental Therapy: A Century of Controversy," provides a historical account of dental therapy, its distribution worldwide, the type of curriculum that is followed for it globally and, in the U.S., the practice expectations of dental therapists and current controversies.
2021 Clinical & Educational Scholarship Showcase Abstracts
Authors: Amintavakoli Niloufar , Spivakovsky Silvia , Attanasi Kim , Rodriguez Cardenas Maria P , DeBartolo Angela , DePaola Cefola Lucretia , Kumar Arthi , Kyles Jason B , Ferraiolo Debra M , Pezzullo Cheryline , Burns Lorel , Stier Kaitlin M , Benvenuto Victoria , Stefanou Lisa , Sefo Dianne , Strange John
Published: April 2024

Fifteen abstracts from the Clinical & Educational Showcase hosted in January 2021 by the Academy of Distinguished Educators elucidate how educators continue coping with the uncertainty that the pandemic has produced, find ways to transcend it, and integrate the lessons we've learned into the educational process as we move out of the pandemic.
İnsan kaynakları yönetiminde yapay zekâ teknolojisinin benimsenmesi üzerine güç alanı analizi
Authors: Kişi Nermin , Özer Mehmet Akif
Published: April 2024

Artan küreselleşme ile birlikte sürekli değişen iş dünyasında, teknolojik yenilikler hayatta kalmanın kritik bir belirleyicisi olarak kabul edilmektedir. Bu nedenle, modern çağın işletmeleri iş süreçlerinde yeni teknolojileri kullanmaya yönelmektedir. Günümüzde en çok tartışılan ve en yaygın kullanılan teknolojilerinden biri yapay zekâdır. İşletmelerin mevcut sistemlerinden bu ileri teknoloji sistemlerine geçişi kapsamlı bir planlama süreci gerektirir. Bu açıdan, işletmelerde yapay zekâya dayalı uygulamaları benimsemede temel faktörleri keşfetmek önemli bir araştırma konusudur. Özellikle, İnsan Kaynakları Yönetimi (İKY)’nde yapay zekâ kullanımına geçiş karmaşık ve zorlu bir süreç olduğundan, bu konuda daha fazla araştırma yapılmasına ihtiyaç duyulmaktadır. Bu çalışmanın amacı, yapay zekâya dayalı İKY uygulamalarının benimsenmesini etkileyen itici ve kısıtlayıcı güçleri belirlemektir. Bu amaca ulaşmak için, çalışmada Güç Alanı Analizi Modeli çerçevesinde sistematik literatür taraması yöntemi kullanılmıştır. Bulgular İKY’de yapay zekânın benimsenmesini teşvik eden temel itici güçlerin örgütsel hazırbulunuşluk ve algılanan faydalar olduğunu ortaya koymuştur. Ayrıca, teknolojik değişime karşı olumsuz tepkiler, veri gizliliği ve güvenliğiyle ilgili endişeler, algoritmik önyargıdan kaynaklanan hatalar ve duygusal zekâ eksikliği İKY’de yapay zekânın benimsenmesinde karşılaşılan kısıtlayıcı güçler olarak tanımlanmıştır.
Heterogeneous risk tolerance, in-groups, and epidemic waves
Authors: Tovissodé Chénangnon Frédéric , Baumgaertner Bert
Published: April 2024

There is a growing interest in the joint modeling of the dynamics of disease and health-related beliefs and attitudes, but coupling mechanisms are yet to be understood. We introduce a model where risk information, which can be delayed, comes in two flavors, including historical risk derived from perceived incidence data and predicted risk information. Our model also includes an interpretation domain where the behavioral response to risk information is subject to in-group pressure. We then simulate how the strength of behavioral reaction impacts epidemic severity as measured by epidemic peak size, number of waves, and final size. Simulated behavioral response is not effective when the level of protection that prophylactic behavior provides is as small as 50% or lower. At a higher level of 75% or more, we see the emergence of multiple epidemic waves. In addition, simulations show that different behavioral response profiles can lead to various epidemic outcomes that are non-monotonic with the strength of reaction to risk information. We also modeled heterogeneity in the response profile of a population and find they can lead to less severe epidemic outcome in terms of peak size.
Smart cities and communities in the GCC region: from top-down city development to more local approaches
Authors: Al-Saidi Mohammad , Zaidan Esmat
Published: April 2024

City planners are seeking ways to incorporate human-centric urban projects that combine smart technology, good infrastructure and people’s perception as well as their participation. Smart city developments are increasingly being applied to smaller scales at which communities can co-design hard infrastructure and the resulting services. Experiences from city projects at this level have produced a plethora of designs, challenges and success factors, particularly from cases in countries with long legacies in city development. In the Gulf Cooperation Council region, young cities and new planned cities house the bulk of the population and face environmental challenges related to urban segregation, urban sprawl and large consumption footprints. The political-economy of this region in terms of central urban planning and government-led economic development offers valuable insights on the limits and challenges in implementing projects related to smart and connected communities (SCC). SCC as a label for smart urban interventions towards more connectedness between hard and soft (human-related) infrastructure can provide opportunities for participatory and sustainable urban planning in the region. This paper analyzes the role of community-level interventions within the smart city policies of Gulf countries. It shows that only few local-level projects exist, while the barriers to large-scale SCC initiatives are related to demographics, community characteristics, technological sophistication, lack of conducive regulations, and the prevalence of central urban planning. The success of local smart city approaches in the Gulf is dependent on public leadership in terms of clearing obstacles, designing broader strategies, and expanding projects to include the residential sector. Considering the potential of SCC projects to enhance the transition to sustainability in the predominantly urban Gulf societies, broader engagement of local governments, utilities, and community-level developers can result in tangible benefits in terms of more sustainable, smart and tailored local services.
Exploring theophylline-1,2,4-triazole tethered N-phenylacetamide derivatives as antimicrobial agents: unraveling mechanisms via structure-activity relationship, in vitro validation, and in silico insights
Authors: Saeed Sadaf , Shahzadi Irum , Zahoor Ameer Fawad , Al-Mutairi Aamal A. , Kamal Shagufta , Faisal Shah , Irfan Ali , Al-Hussain Sami A. , Muhammed Muhammed Tilahun , Zaki Magdi E. A.
Published: April 2024

Theophylline, a nitrogen-containing heterocycle, serves as a promising focal point for medicinal researchers aiming to create derivatives with diverse pharmacological applications. In this work, we present an improved synthetic method for a range of theophylline-1,2,4-triazole-S-linked N-phenyl acetamides (4a‒g) utilizing ultrasound-assisted synthetic approach. The objective was to assess the effectiveness of synthesized theophylline-1,2,4-triazoles (4a‒g) as inhibitors of HCV serine protease and as antibacterial agents against B. subtilis QB-928 and E. coli AB-274. Theophylline-1,2,4-triazoles were obtained in good to excellent yields (69%–95%) in a shorter time than conventional approach. 4-Chlorophenyl moiety containing theophylline-1,2,4-triazole 4c displayed significantly higher inhibitory activity against HCV serine protease enzyme (IC50 = 0.015 ± 0.25 mg) in comparison to ribavirin (IC50 = 0.165 ± 0.053 mg), but showed excellent binding affinity (−7.55 kcal/mol) with the active site of serine protease, better than compound 4c (−6.90 kcal/mol) as well as indole-based control compound 5 (−7.42 kcal/mol). In terms of percentage inhibition of serine protease, 2-chlorophenyl compound 4b showed the maximum percentage inhibition (86%), more than that of the 3,4-dichlorophenyl compound 4c (76%) and ribavirin (81%). 3,4-Dimethylphenyl-based theophylline-1,2,4-triazole 4g showed the lowest minimum inhibitory concentration (MIC = 0.28 ± 0.50 μg/mL) against the B. subtilis bacterial strain as compared to the standard drug penicillin (MIC = 1 ± 1.50 μg/mL). The other 4-methylphenyl theophylline-1,2,4-triazole 4e (MIC = 0.20 ± 0.08 μg/mL) displayed the most potent antibacterial potential against E. coli in comparison to the standard drug penicillin (MIC = 2.4 ± 1.00 μg/mL). Molecular docking studies further helped in an extensive understanding of all of the interactions between compounds and the enzyme active site, and DFT studies were also employed to gain insights into the molecular structure of the synthesized compounds. The results indicated that theophylline-linked triazole derivatives 4b and 4c showed promise as leading contenders in the fight against the HCV virus. Moreover, compounds 4e and 4g demonstrated potential as effective chemotherapeutic agents against E. coli and B. subtilis, respectively. To substantiate these findings, additional in vivo studies and clinical trials are imperative, laying the groundwork for their integration into future drug design and development.
Integrative computational approaches for discovery and evaluation of lead compound for drug design
Authors: Naithani Utkarsha , Guleria Vandana
Published: April 2024

In the drug discovery and development, the identification of leadcompoundsplaysa crucial role in the quest for novel therapeutic agents. Leadcompounds are the initial molecules that show promising pharmacological activity againsta specific target and serve as the foundation for drug development. Integrativecomputational approaches have emerged as powerful tools in expediting this complex andresource-intensive process. They enable the efficient screening of vast chemical librariesand the rational design of potential drug candidates, significantly accelerating the drugdiscoverypipeline. This review paper explores the multi-layered landscape of integrative computationalmethodologies employed in lead compound discovery and evaluation. These approaches include various techniques, including molecular modelling, cheminformatics, structure-based drug design (SBDD), high-throughput screening, molecular dynamics simulations, ADMET (absorption, distribution, metabolism, excretion, and toxicity) prediction, anddrug-target interaction analysis. By revealing the critical role ofintegrative computational methods, this review highlights their potential to transformdrug discovery into a more efficient, cost-effective, and target-focused endeavour, ultimately paving the way for the development of innovative therapeutic agents to addressa multitude of medical challenges.
The landslide traces inventory in the transition zone between the Qinghai-Tibet Plateau and the Loess Plateau: a case study of Jianzha County, China
Authors: Li Tao , Xu Chong , Li Lei , Xu Jixiang
Published: April 2024

The upper reaches of the Yellow River in China, influenced by erosion of the Yellow River and tectonic activities, are prone to landslides. Therefore, it is necessary to investigate the existing landslide traces. Based on visual interpretation on high-resolution satellite images and terrain data, supplemented and validated by existing landslide records, this paper prepared the most complete and detailed landslide traces inventory in Jianzha County, Huangnan Tibetan Autonomous Prefecture, Qinghai Province, to date. The results indicate that within the study area of 1714 km2, there are at least 713 landslide traces, ranging in scale from 3,556 m2 to 11.13 km2, with a total area of 134.46 km2. The total landslide area excluding the overlap area is 126.30 km2. The overall landslide point density and area density in the study area are 0.42 km-2 and 7.37% respectively. The maximum point density and maximum area density of landslide traces in the area are as high as 5.69 km-2 and 98.0% respectively. The landslides are primarily distributed in the relatively low-elevation northeastern part of Jianzha County, characterized mainly by large-scale loess landslides, with 14 landslides exceeding 1×106 m2. This inventory not only supplements the landslide trace data in the transition zone between the Qinghai-Tibet Plateau and the Loess Plateau, but also provides an important basis for subsequent landslide risk zoning, response to climate change, and landscape evolution. Additionally, it holds significant reference value for compiling landslide inventories in similar geological environments.
Outcomes of endoscopic ultrasound-guided ablation and minimally invasive surgery in the treatment of pancreatic insulinoma: a systematic review and meta-analysis
Authors: Xiao Dan , Zhu Li , Xiong Si , Yan Xu , Jiang Qin , Wang Ao , Jia Yegui
Published: April 2024

Background and aims

Most pancreatic insulinomas can be treated by minimally invasive modalities. The aim of this meta-analysis was to assess the clinical outcomes of endoscopic ultrasound (EUS)-guided ablation and minimally invasive surgery (MIS) in the treatment of pancreatic insulinoma.

Materials and methods

Online databases were searched for relevant studies. The primary aim was to compare the rates of adverse events (AEs) and the secondary aims were to compare the clinical and technical success rates, length of hospital stays, and symptom recurrence rates between EUS and MIS approaches.

Results

Eight studies with 150 patients were identified that reported EUS-guided ablation outcomes, forming the EUS group, and 9 studies with 236 patients reported MIS outcomes, forming the MIS group. The pooled median age of the included patients in the EUS group was greater than that of the MIS group (64.06 vs. 44.98 years old, p < 0.001). Also, the technical success rate was significantly higher in the EUS group (100% vs. 96.6%, p = 0.025), while the clinical success was significantly higher (6%) in the MIS group (94% vs. 98.7%, p = 0.021). The AE rates (18.7% vs. 31.1%, p = 0.012) and severe AE rates (1.3% vs. 7.9%, p = 0.011) were significantly lower in the EUS group. The median length of hospital stay in the EUS group (2.68 days, 95% CI: 1.88–3.48, I2 = 60.3%) was significantly shorter than in the MIS group (7.40 days, 95% CI: 6.22–8.58, I2 = 42.2%, p < 0.001). The recurrence rate was significantly higher in the EUS group (15.3% vs. 1.3%, p < 0.001).

Conclusions

EUS-guided ablation is associated with a lower AE rate and a shorter length of hospital stay, but a higher recurrence rate for the treatment of insulinoma compared with MIS. The EUS approach may be an alternative, even first-line, treatment for poor surgery candidates.
Exploring a path of vegetation restoration best suited for a photovoltaic plant in the Hobq desert
Authors: Cai Jiale , Meng Zhongju , Meng Ruibing , Li Haonian , Chen Xiaoyan , Ren Xiaomeng , Guo Lijun , Hao Meijun
Published: April 2024

To investigate the soil improvement effects of different vegetation restoration measures during the operation and maintenance of photovoltaic power plants in the Hobq Desert. This study determined the soil grain size composition and soil nutrient content of 0–5 cm under four vegetation restoration measures and within the mechanical sand barriers by laser diffraction techniques and chemical experiments. The results showed that: (1) Soil particle size composition in the study area is predominantly sandy, with 1.02%, 6.63%, 5.34% and 2.61% less sand inLeymus chinensis(YC),Glycyrrhiza uralensis(GC),Artemisia ordosica Krasch(YH) andHedysarum scoparium(HB), respectively, compared to mechanical sand barriers (CK). Soil particle distributions of YC, GC, and HB showed better sorting and more concentrated patterns, and all four vegetation restoration measures had higher fractal dimensions than CK. (2) AN (Alkali-hydrolyzable Nitrogen) content performance: The content of YC, GC, and HB was significantly higher than that of YH and CK, AP (Available Phosphorus) content did not differ significantly between measures, AK (Available Potassium) content was higher than that of CK in all measures, with YC content being the highest and SOM content being the highest for GC. (3) Soil total nutrient is unstable across vegetation restoration measures. Overall, the performance showed that the three vegetation restoration measures, YC, GC, and HB, were more evident in the soil total nutrient content improvement. Each particle size characteristic parameter and soil nutrient response vegetation restoration measures ameliorate sandy soil in PV power plants during operation and maintenance. YC and GC have the most apparent effect on soil amelioration.
Identification and characterization of endogenous retroviruses upon SARS-CoV-2 infection
Authors: Guo Xuefei , Zhao Yang , You Fuping
Published: April 2024

Endogenous retroviruses (ERVs) derived from the long terminal repeat (LTR) family of transposons constitute a significant portion of the mammalian genome, with origins tracing back to ancient viral infections. Despite comprising approximately 8% of the human genome, the specific role of ERVs in the pathogenesis of COVID-19 remains unclear. In this study, we conducted a genome-wide identification of ERVs in human peripheral blood mononuclear cells (hPBMCs) and primary lung epithelial cells from monkeys and mice, both infected and uninfected with SARS-CoV-2. We identified 405, 283, and 206 significantly up-regulated transposable elements (TEs) in hPBMCs, monkeys, and mice, respectively. This included 254, 119, 68, and 28 ERVs found in hPBMCs from severe and mild COVID-19 patients, monkeys, and transgenic mice expressing the human ACE2 receptor (hACE2) and infected with SARS-CoV-2. Furthermore, analysis using the Genomic Regions Enrichment of Annotations Tool (GREAT) revealed certain parental genomic sequences of these up-regulated ERVs in COVID-19 patients may be involved in various biological processes, including histone modification and viral replication. Of particular interest, we identified 210 ERVs specifically up-regulated in the severe COVID-19 group. The genes associated with these differentially expressed ERVs were enriched in processes such as immune response activation and histone modification. HERV1_I-int: ERV1:LTR and LTR7Y: ERV1:LTR were highlighted as potential biomarkers for evaluating the severity of COVID-19. Additionally, validation of our findings using RT-qPCR in Bone Marrow-Derived Macrophages (BMDMs) from mice infected by HSV-1 and VSV provided further support to our results. This study offers insights into the expression patterns and potential roles of ERVs following viral infection, providing a valuable resource for future studies on ERVs and their interaction with SARS-CoV-2.
Memory B-cell derived donor-specific antibodies do not predict outcome in sensitized kidney transplant recipients: a retrospective single-center study
Authors: Altulea Dania , van den Born Joost C. , Diepstra Arjan , Bungener Laura , Terpstra Dagmar , Hepkema Bouke G. , Lammerts Rosa , Heeringa Peter , Heidt Sebastiaan , Otten Henny , Reteig Leon , Karahan Gonca E. , Berger Stefan P. , Sanders Jan-Stephan
Published: April 2024

Background

Repeated exposure to sensitizing events can activate HLA-specific memory B cells, leading to the production of donor-specific memory B cell antibodies (DSAm) that pose a risk for antibody-mediated rejection (ABMR) in kidney transplant recipients (KTRs). This single-center retrospective study aimed to identify DSAm and assess their association with outcomes in a cohort of KTRs with pretransplant serum donor-specific antibodies (DSA).

Methods

We polyclonally activated pretransplant peripheral blood mononuclear cells (PBMCs) from 60 KTRs in vitro, isolated and quantified IgG from the culture supernatant using ELISA, and analyzed the HLA antibodies of eluates with single antigen bead (SAB) assays, comparing them to the donor HLA typing for potential DSAm. Biopsies from 41 KTRs were evaluated for rejection based on BANFF 2019 criteria.

Results

At transplantation, a total of 37 DSAm were detected in 26 of 60 patients (43%), of which 13 (35%) were found to be undetectable in serum. No significant association was found between pretransplant DSAm and ABMR (P=0.53). Similar results were observed in a Kaplan–Meier analysis for ABMR within the first year posttransplant (P=0.29). Additionally, MFI levels of DSAm showed no significant association with ABMR (P=0.28).

Conclusion

This study suggests no significant association between DSAm and biopsy-proven clinical ABMR. Further prospective research is needed to determine whether assessing DSAm could enhance existing immunological risk assessment methods for monitoring KTRs, particularly in non-sensitized KTRs.
Granzymes in health and diseases: the good, the bad and the ugly
Authors: Cigalotto Lavinia , Martinvalet Denis
Published: April 2024

Granzymes are a family of serine proteases, composed of five human members: GA, B, H, M and K. They were first discovered in the 1980s within cytotoxic granules released during NK cell- and T cell-mediated killing. Through their various proteolytic activities, granzymes can trigger different pathways within cells, all of which ultimately lead to the same result, cell death. Over the years, the initial consideration of granzymes as mere cytotoxic mediators has changed due to surprising findings demonstrating their expression in cells other than immune effectors as well as new intracellular and extracellular activities. Additional roles have been identified in the extracellular milieu, following granzyme escape from the immunological synapse or their release by specific cell types. Outside the cell, granzyme activities mediate extracellular matrix alteration via the degradation of matrix proteins or surface receptors. In certain contexts, these processes are essential for tissue homeostasis; in others, excessive matrix degradation and extensive cell death contribute to the onset of chronic diseases, inflammation, and autoimmunity. Here, we provide an overview of both the physiological and pathological roles of granzymes, highlighting their utility while also recognizing how their unregulated presence can trigger the development and/or worsening of diseases.
Numerical simulation of hydrodynamics of ocean-observation-used remotely operated vehicle
Authors: Zhang Dapeng , Zhao Bowen , Zhang Yi , Zhou Nan
Published: April 2024

Remotely operated vehicle is the most widely used underwater robot and can work safely and steadily in complex environments compared to autonomous underwater vehicle and other types. It has obvious advantages in operation time and plays a significant function in marine engineering equipment. Hydrodynamic coefficients are the coefficients of ROV motion equation. In order to simulate the motion and predict the performance of a ROV, the hydrodynamic coefficients must be determined first. The motion mathematical model of remotely operated vehicles is also established, and the hydrodynamic dynamics of the vehicles are simulated using the finite volume method by combining overset mesh technology and governing equations. Finally, a simulation and verification of the standard model SUBOFF model and the calculation process’s dependability are also conducted. The primary hydrodynamic coefficients of the ROV were derived through the simulation data fitting process. The results showed that the ROV’s asymmetry results in an obvious disparity in pressure resistance between the forward and backward sailing, ascending and descending motions, and this disparity becomes significantly greater as the velocity increases. This method confirmed the accuracy of the hydrodynamic simulation computation of the remotely operated vehicle and served as a guide for the maneuverability and design of the vehicle.
DeepSTARia: enabling autonomous, targeted observations of ocean life in the deep sea
Authors: Barnard Kevin , Daniels Joost , Roberts Paul L. D. , Orenstein Eric C. , Masmitja Ivan , Takahashi Jonathan , Woodward Benjamin , Katija Kakani
Published: April 2024

The ocean remains one of the least explored places on our planet, containing myriad life that are either unknown to science or poorly understood. Given the technological challenges and limited resources available for exploring this vast space, more targeted approaches are required to scale spatiotemporal observations and monitoring of ocean life. The promise of autonomous underwater vehicles to fulfill these needs has largely been hindered by their inability to adapt their behavior in real-time based on what they are observing. To overcome this challenge, we developed Deep Search and Tracking Autonomously with Robotics (DeepSTARia), a class of tracking-by-detection algorithms that integrate machine learning models with imaging and vehicle controllers to enable autonomous underwater vehicles to make targeted visual observations of ocean life. We show that these algorithms enable new, scalable sampling strategies that build on traditional operational modes, permitting more detailed (e.g., sharper imagery, temporal resolution) autonomous observations of underwater concepts without supervision and robust long-duration object tracking to observe animal behavior. This integration is critical to scale undersea exploration and represents a significant advance toward more intelligent approaches to understanding the ocean and its inhabitants.
Iron-organic carbon coprecipitates reduce nitrification by restricting molybdenum in agricultural soils
Authors: Slimani Imane , Doane Timothy , Zhu-Barker Xia , Lazicki Patricia , Lybrand Rebecca A. , Zaharescu Dragos G. , Horwath William
Published: April 2024

Nitrification converts ammonium (NH4+) to nitrate (NO3−) using metalloenzymes, the activity of which depends on iron (Fe), molybdenum (Mo), and copper (Cu) availability. Iron-organic carbon coprecipitates (or Fe-OC flocs) are key byproducts of wastewater treatment industry and natural components of soil that may affect nitrification by changing the bioavailability of these metals. Here, we used flocs of different chemistry (aromatic and aliphatic) and known Fe and C composition to investigate their effects on nitrification in soils along a soil C gradient. Both aromatic and aliphatic flocs reduced net nitrification, but the magnitude of their effect was more pronounced in soils with low C content as opposed to those with high C content. Within each soil, both flocs reduced net nitrification similarly. In the presence of flocs, the bioavailability of Mo (assessed by changes in the concentration of water-soluble Mo) was dramatically decreased in low C soils, possibly because Mo was incorporated into or adsorbed to flocs or their decomposition products. In contrast, Mo bioavailability in high C soils was decreased to a lesser extent by flocs, likely because organic matter limited floc adsorption capacity and released Mo through mineralization. The depletion of bioavailable Mo by flocs in agricultural soils has the potential to impede soil nitrification and extend the residence time of NH4+ and its availability to plants and microbes.
Tensile properties and constitutive model of cost-effective multiscale hybrid fiber reinforced strain hardening cementitious composites
Authors: Hou Jin , Bai Jianjun , Mou Hongmei , Xiang Zhisuo
Published: April 2024

To enhance the mechanical properties and cost-effectiveness of conventional polyvinyl alcohol fiber reinforced strain hardening cementitious composite (PVA-SHCC), a modified version called multiscale hybrid fiber reinforced SHCC (MsHySHCC) was developed. This new composite incorporates a combination of steel fiber, PVA fiber and calcium carbonate (CaCO3) whisker. Uniaxial direct tensile behaviors (stress-strain relationship, tensile strength, tensile deformation capacity and tensile toughness) of designed MsHySHCCs were investigated and evaluated. The results show that the PVA fibers dominate the ductile behavior and the steel fibers and CaCO3 whiskers effectively affect the strength of MsHySHCCs. The PVA fibers can be partially replaced by CaCO3 whisker and steel fiber, along with an increase in tensile strength and ductility of designed composites. The findings suggest that the configuration of MsHySHCC proves to be a viable approach in simultaneously enhancing the strength and ductility of PVA-SHCC. A semi-theoretical prediction model for tensile constitutive relationship was derived. The comparison of the theoretical results with the experimental data shows that this semi-theoretical model is applicable for determining the tensile constitutive relationship of PVA-SHCCs and MsHySHCCs.
The value of pre-hospital trauma life support courses for medical personnel—a questionnaire study
Authors: Teuben Michel Paul Johan , Löhr Nikolaus , Shehu Alba , Berk Till , Jensen Kai Oliver , Mikova Ester , Brüesch Martin , Müller Stephan , Pfeifer Roman , Mica Ladislav , Pape Hans Christoph , Sprengel Kai
Published: April 2024

Background

The aim of the study was to determine the impact that PHTLS® course participation had on self-confidence of emergency personnel, regarding the pre-hospital treatment of patients who had suffered severe trauma. Furthermore, the goal was to determine the impact of specific medical profession, work experience and prior course participation had on the benefits of PHTLS® training.

Methods

A structured questionnaire study was performed. Healthcare providers from local emergency services involved in pre-hospital care in the metropolitan area of Zurich (Switzerland, Europe) who completed a PHTLS® course were included. Altered self-confidence, communication, and routines in the treatment of severe trauma patients were examined. The impact of prior course participation, work experience and profession on course benefits were evaluated.

Results

The response rate was 76%. A total of 6 transport paramedics (TPs), 66 emergency paramedics (EPs) and 15 emergency doctors (EDs) were included. Emergency paramedics had significantly more work experience compared with EDs (respectively 7.1 ± 5.7 yrs. vs. 4.5 ± 2.1 yrs., p = 0.004). 86% of the participants reported increased self-confidence in the pre-hospital management of severe trauma upon PHTLS® training completion. Moreover, according to 84% of respondents, extramural treatment of trauma changed upon course completion. PHTLS® course participants had improved communication in 93% of cases. This was significantly more frequent in EPs than TPs (p = 0.03). Multivariable analysis revealed emergency paramedics benefit the most from PHTLS® course participation.

Conclusion

The current study shows that PHTLS® training is associated with improved self-confidence and enhanced communication, with regards to treatment of severe trauma patients in a pre-hospital setting, among medical emergency personnel. Additionally, emergency paramedics who took the PHTLS® course improved in overall self-confidence. These findings imply that all medical personal involved in the pre-hospital care of trauma patients, in a metropolitan area in Europe, do benefit from PHTLS® training. This was independent of the profession, previous working experience or prior alternative course participation.
Clostridium butyricum isolated from giant panda can attenuate dextran sodium sulfate-induced colitis in mice
Authors: Yu Shuran , Xie Junjin , Guo Qiang , Yan Xia , Wang Yuxiang , Leng Tangjian , Li Lin , Zhou Jielong , Zhang Wenping , Su Xiaoyan
Published: April 2024

Objective

Probiotics are beneficial to the intestinal barrier, but few studies have investigated probiotics from giant pandas. This study aims to explore the preventive effects of giant panda-derived Clostridium butyricum on dextran sodium sulfate (DSS)-induced colitis in mice.

Methods

Clostridium butyricum was administered to mice 14 days before administering DSS treatment to induce enteritis.

Results

Clostridium butyricum B14 could more effectively prevent colitis in mice than C. butyricum B13. C. butyricum B14 protected the mouse colon by decreasing the histology index and serum interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-α) levels, which improved intestinal inflammation-related symptoms. In addition, the treatment led to the regulation of the expression of Tifa, Igkv12-89, and Nr1d1, which in turn inhibited immune pathways. The expression of Muc4, Lama3, Cldn4, Cldn3, Ocln, Zo1, Zo2, and Snai is related the intestinal mucosal barrier. 16S sequencing shows that the C. butyricum B14 significantly increased the abundance of certain intestinal probiotics. Overall, C. butyricum B14 exerted a preventive effect on colitis in mice by inhibiting immune responses, enhancing the intestinal barrier and increasing the abundance of probiotic species. Thus, C. butyricum B14 administration helps regulate the balance of the intestinal microecology. It can suppress immune pathways and enhance barrier-protective proteins.
Liver kinase B-1 modulates the activity of dopamine neurons in the ventral tegmental area and regulates social memory formation
Authors: Yu Meng , Sun Fengjiao , Xiang Guo , Zhang Yuhan , Wang Xuejun , Liu Xia , Huang Bin , Li Xingang , Zhang Di
Published: April 2024

Social memory is the ability to discriminate between familiar and unknown conspecifics. It is an important component of social cognition and is therefore essential for the establishment of social relationships. Although the neural circuit mechanisms underlying social memory encoding have been well investigated, little focus has been placed on the regulatory mechanisms of social memory processing. The dopaminergic system, originating from the midbrain ventral tegmental area (VTA), is a key modulator of cognitive function. This study aimed to illustrate its role in modulating social memory and explore the possible molecular mechanisms. Here, we show that the activation of VTA dopamine (DA) neurons is required for the formation, but not the retrieval, of social memory. Inhibition of VTA DA neurons before social interaction, but not 24 h after social interaction, significantly impaired social discrimination the following day. In addition, we showed that the activation of VTA DA neurons was regulated by the serine/threonine protein kinase liver kinase B1 (Lkb1). Deletion of Lkb1 in VTA DA neurons reduced the frequency of burst firing of dopaminergic neurons. Furthermore, Lkb1 plays an important role in regulating social behaviors. Both genetic and virus-mediated deletions of Lkb1 in the VTA of adult mice impaired social memory and subsequently attenuated social familiarization. Altogether, our results provide direct evidence linking social memory formation to the activation of VTA DA neurons in mice and illustrate the crucial role of Lkb1 in regulating VTA DA neuron function.
Perinatal exposure to atazanavir-based antiretroviral regimens in a mouse model leads to differential long-term motor and cognitive deficits dependent on the NRTI backbone
Authors: Dhume Shreya H. , Balogun Kayode , Sarkar Ambalika , Acosta Sebastian , Mount Howard T. J. , Cahill Lindsay S. , Sled John G. , Serghides Lena
Published: April 2024

Background

Combination antiretroviral therapy (ART) use in pregnancy has been pivotal in improving maternal health and reducing perinatal HIV transmission. However, children born HIV-exposed uninfected fall behind their unexposed peers in several areas including neurodevelopment. The contribution of in utero ART exposure to these deficits is not clear. Here we present our findings of neurocognitive outcomes in adult mice exposed in utero to ART.

Methods

Dams were treated with a combination of ritonavir-boosted atazanavir with either abacavir plus lamivudine (ABC/3TC + ATV/r) or tenofovir disoproxil fumarate plus emtricitabine (TDF/FTC + ATV/r), or water as a control, administered daily from day of plug detection to birth. Offspring underwent a battery of behavioral tests that investigated motor performance and cognition starting at 6-weeks of age and ending at 8 months. Changes in brain structure were assessed using magnetic resonance imaging and immunohistochemistry. Expression of genes involved in neural circuitry and synaptic transmission were assessed in the hippocampus, a region strongly associated with memory formation, using qPCR.

Findings

Pups exposed to TDF/FTC + ATV/r showed increased motor activity and exploratory drive, and deficits in hippocampal-dependent working memory and social interaction, while pups exposed to ABC/3TC + ATV/r showed increased grooming, and deficits in working memory and social interaction. Significant volumetric reductions in the brain were seen only in the ABC/3TC + ATV/r group and were associated with reduced neuronal counts in the hippocampus. Altered neurotransmitter receptor mRNA expression as well as changes in expression of the neurotrophic factor BDNF and its receptors were observed in both ART-exposed groups in a sex-dependent manner.

Interpretation

In our model, in utero ART exposure had long-term effects on brain development and cognitive and motor outcomes in adulthood. Our data show that neurological outcomes can be influenced by the type of nucleoside reverse transcriptase inhibitor backbone of the regimen and not just the base drug, and display sex differences.
Association between the oxidative balance score and low muscle mass in middle-aged US adults
Authors: Chen Kun , Yin Qiang , Guan Jiangan , Yang Jingwen , Ma Yuan , Hu Yu , Chen Chan , Chen Wenwen
Published: April 2024

Background

Oxidative Balance Score (OBS) is a tool for assessing the oxidative stress-related exposures of diet and lifestyle. The study aimed to investigate the association between OBS and low muscle mass.

Methods

Overall, 6,307 individuals over the age of 18 were assessed using data from the 2011 to 2018 National Health and Nutrition Examination Survey (NHANES). Weighted logistic regression and models were used, together with adjusted models.

Results

There was a negative relationship between OBS and low muscle mass [odds ratio (OR): 0.96, 95% confidence interval (CI): 0.94–0.97, p< 0.0001] using the first OBS level as reference. The values (all 95% CI) were 0.745 (0.527–1.054) for the second level, 0.650 (0.456–0.927) for the third level, and 0.326 (0.206–0.514) for the fourth level (P for trend <0.0001). Independent links with low muscle mass were found for diet and lifestyle factors. A restricted cubic spline model indicated a non-linear association between OBS and low muscle mass risk (P for non-linearity<0.05). In addition, the inflection points of the nonlinear curves for the relationship between OBS and risk of low muscle mass were 20.

Conclusion

OBS and low muscle mass were found to be significantly negatively correlated. By modulating oxidative balance, a healthy lifestyle and antioxidant rich diet could be a preventive strategy for low muscle mass.
A retrospective study of the efficacy and safety of levofloxacin in children with severe infection
Authors: Junqi Zhang , Jie Cai , Jinglin Wang , Jinmiao Lu , Guoping Lu , Yi Wang , Zhiping Li
Published: April 2024

Objectives

Levofloxacin is widely used because of its broad-spectrum antimicrobial activity and convenient dosing schedule. However, the relevance of its use in children remains to be investigated. The purpose of this study is to investigate the efficacy and safety of levofloxacin use in children with severe infections.

Methods

We conducted a retrospective observational study of patients <18 years of age who received levofloxacin intravenously in the Pediatric Intensive Care Unit (PICU) of our hospital during the period between 2021 and 2022. Patient demographics, course characteristics, clinical effectiveness, and adverse event correlations were extracted through a retrospective tabular review.

Results

We included 25 patients treated with 28 courses of levofloxacin. The mean age of these children treated with levofloxacin was 4.41 years. Conversion of pathogenic microbiological test results to negative after levofloxacin treatment was detected in 11 courses (39.29%). A decrease in inflammatory markers, white blood cell or C-reactive protein counts, was detected in 18 courses (64.29%). A total of 57 adverse events occurred during the treatment period, of which 21 were possibly related to levofloxacin and no adverse events were probably related to levofloxacin.

Conclusion

The effectiveness of levofloxacin use in children with serious infections is promising, especially for the treatment of multidrug-resistant bacteria. Adverse events occurring during the initiation of levofloxacin therapy in children are reported to be relatively common, but in this study, only a small percentage of them were possibly related to levofloxacin, and none of them were highly possibly related to levofloxacin.
An 8-month adapted motor activity program in a young CMT1A male patient
Authors: Bottoni Giorgio , Crisafulli Oscar , Pisegna Caterina , Serra Marco , Brambilla Sara , Feletti Fausto , Cremonte Giovanni , D’Antona Giuseppe
Published: April 2024

Background:

It is unclear whether prolonged periods of training can be well tolerated. In Charcot-Marie Tooth disease (CMT). We report the effects of an 8-month, adapted motor activity (AMA) program in a 16-years-old CMT1A male patient. The program included strength, mobility, and balance training (two sessions per week, 1 h per session).

Measures:

Walking ability and walking velocity (Six-Minute Walking Test—6MWT, Ten Meters Walking Test—10 mW T), balance (Y-Balance Test—YBT, Berg Balance Scale—BBS), functional mobility (Short Physical Performance Battery—Short physical performance battery), fatigue (Checklist Individual strength questionnaire - CIS20R), health and quality of life (Short Form Health Survey 36 questionnaire—SF-36) were evaluated in three moments: before (T0), after 5 (T1) and 8 (T2) months of adapted motor activity. Dorsal and plantar foot flexion strength (Maximal Voluntary Contraction—maximum voluntary contraction) and neuromuscular functions (Electromyography—sEMG, interpolated twitch technique—ITT) were measured at T1 and T2.

Results:

Relative to T0, an amelioration of walking ability (6MWT, +9,3%) and balance (with improvements on Y-balance composite normalized mean reach of the right and left limb of 15,3% and 8,5%, respectively) was appreciable. Relative to T1, an increase in foot strength in three out of four movements (right plantar flexion, +39,3%, left plantar flexion, +22,7%, left dorsal flexion, 11,5%) was observed. Concerning voluntary muscle activation, a greater recruitment in the left, unlike right, medial gastrocnemius was observed.

Conclusion:

Results suggest the safety of an 8-month AMA program in a young patient affected by CMT1A.
Individualized positive end-expiratory pressure reduces driving pressure in obese patients during laparoscopic surgery under pneumoperitoneum: a randomized clinical trial
Authors: Xavier Tiago Batista , Coelho Leonardo Vicente , Ferreira Daniel Antonio Lopes , Cota y Raposeiras José Manuel , Duran Marcelo Sampaio , Silva Leticia Almeida , Motta-Ribeiro Gabriel Casulari da , Camilo Luciana Moisés , Carvalho Alysson Roncally Silva , Silva Pedro Leme
Published: April 2024

Introduction

During pneumoperitoneum (PNP), airway driving pressure (ΔPRS) increases due to the stiffness of the chest wall and cephalic shift of the diaphragm, which favors atelectasis. In addition, depending on the mechanical power (MP) formulas, they may lead to different interpretations.

Methods

Patients >18 years of age with body mass index >35 kg/m2 were included in a single-center randomized controlled trial during their admission for bariatric surgery by abdominal laparoscopy. Intra-abdominal pressure was set at 15 mmHg at the pneumoperitoneum time point (PNP). After the recruitment maneuver, the lowest respiratory system elastance (ERS) was detected during the positive end-expiratory pressure (PEEP) step-wise decrement. Patients were randomized to the 1) CTRL group: ventilated with PEEP of 5 cmH2O and 2) PEEPIND group: ventilated with PEEP value associated with ERS that is 5% higher than its lowest level. Respiratory system mechanics and mean arterial pressure (MAP) were assessed at the PNP, 5 min after randomization (T1), and at the end of the ventilation protocol (T2); arterial blood gas was assessed at PNP and T2. ΔPRS was the primary outcome. Three MP formulas were used: MPA, which computes static PEEP × volume, elastic, and resistive components; MPB, which computes only the elastic component; and MPC, which computes static PEEP × volume, elastic, and resistive components without inspiratory holds.

Results

Twenty-eight patients were assessed for eligibility: eight were not included and 20 patients were randomized and allocated to CTRL and PEEPIND groups (n = 10/group). The PEEPIND ventilator strategy reduced ΔPRS when compared with the CTRL group (PEEPIND, 13 ± 2 cmH2O; CTRL, 22 ± 4 cmH2O; p < 0.001). Oxygenation improved in the PEEPIND group when compared with the CTRL group (p = 0.029), whereas MAP was comparable between the PEEPIND and CTRL groups. At the end of surgery, MPA and MPB were correlated in both the CTRL (rho = 0.71, p = 0.019) and PEEPIND (rho = 0.84, p = 0.020) groups but showed different bias (CTRL, −1.9 J/min; PEEPIND, +10.0 J/min). At the end of the surgery, MPA and MPC were correlated in both the CTRL (rho = 0.71, p = 0.019) and PEEPIND (rho = 0.84, p = 0.020) groups but showed different bias (CTRL, −1.9 J/min; PEEPIND, +10.0 J/min).

Conclusion

Individualized PEEP was associated with a reduction in ΔPRS and an improvement in oxygenation with comparable MAP. The MP, which solely computes the elastic component, better reflected the improvement in ΔPRS observed in the individualized PEEP group.

Clinical Trial Registration:

The protocol was registered at the Brazilian Registry of Clinical Trials (U1111-1220-7296).