bluuebunny/crossref_metadata_2025 huggingface dataset:
Content Estimation of Foreign Fibers in Cotton Based on Deep Learning
Authors: Wei Wei , Zhang Chen , Deng Dexiang
Published: October 2020

Cotton foreign fibers directly affect the quality of a textile product; the less foreign fibers in raw cotton, the higher the quality grade of the textile product. Based on the foreign fiber clean machine, this paper proposed an evaluation method of foreign fiber content using deep learning. First of all, a large number of images of foreign fibers were collected from different production lines and annotated to obtain the mask image dataset of foreign fibers. Secondly, by comparing the image segmentation algorithm based on deep learning, tests showed that U-Net has a better performance on different segment metrics evaluations, and U-Net is improved to realize the real-time segmentation of foreign fiber images. The actual size of the foreign fiber could be calculated through the combination of the segment result and the mechanical parameters of the machine. Finally, the test results showed that the relative error between the estimated size and the actual size was less than 4%. After the prototype test, the algorithm was deployed on the actual production line and, by comparing the algorithm data in a random time with the actual foreign fiber statistical data, the overall error was less than 2%. The test showed that the new evaluation method can fully reflect the content of foreign fiber in raw cotton.
Assessment of the Steering Precision of a Hydrographic USV along Sounding Profiles Using a High-Precision GNSS RTK Receiver Supported Autopilot
Authors: Marchel Łukasz , Specht Cezary , Specht Mariusz
Published: October 2020

Unmanned Surface Vehicles (USV) are increasingly used to perform numerous tasks connected with measurements in inland waters and seas. One of such target applications is hydrography, where traditional (manned) bathymetric measurements are increasingly often realized by unmanned surface vehicles. This pertains especially to restricted or hardly navigable waters, in which execution of hydrographic surveys with the use of USVs requires precise maneuvering. Bathymetric measurements should be realized in a way that makes it possible to determine the waterbody’s depth as precisely as possible, and this requires high-precision in navigating along planned sounding profiles. This paper presents research that aimed to determine the accuracy of unmanned surface vehicle steering in autonomous mode (with a Proportional-Integral-Derivative (PID) controller) along planned hydrographic profiles. During the measurements, a high-precision Global Navigation Satellite System (GNSS) Real Time Kinematic (RTK) positioning system based on a GNSS reference station network (positioning accuracy: 1–2 cm, p = 0.95) and a magnetic compass with the stability of course maintenance of 1°–3° Root Mean Square (RMS) were used. For the purpose of evaluating the accuracy of the vessel’s path following along sounding profiles, the cross track error (XTE) measure, i.e., the distance between an USV’s position and the hydrographic profile, calculated transversely to the course, was proposed. The tests were compared with earlier measurements taken by other unmanned surface vehicles, which followed the exact same profiles with the use of much simpler and low-cost multi-GNSS receiver (positioning accuracy: 2–2.5 m or better, p = 0.50), supported with a Fluxgate magnetic compass with a high course measurement accuracy of 0.3° (p = 0.50 at 30 m/s). The research has shown that despite the considerable difference in the positioning accuracy of both devices and incomparably different costs of both solutions, the authors proved that the use of the GNSS RTK positioning system, as opposed to a multi-GNSS system supported with a Fluxgate magnetic compass, influences the precision of USV following sounding profiles to an insignificant extent.
Oriented Fermentation of Food Waste towards High-Value Products: A Review
Authors: Wang Qiao , Li Huan , Feng Kai , Liu Jianguo
Published: October 2020

Food waste has a great potential for resource recovery due to its huge yield and high organic content. Oriented fermentation is a promising method with strong application prospects due to high efficiency, strong robustness, and high-value products. Different fermentation types lead to different products, which can be shifted by adjusting fermentation conditions such as inoculum, pH, oxidation-reduction potential (ORP), organic loading rate (OLR), and nutrients. Compared with other types, lactic acid fermentation has the lowest reliance on artificial intervention. Lactic acid and volatile fatty acids are the common products, and high yield and high purity are the main targets of food waste fermentation. In addition to operational parameters, reactors and processes should be paid more attention to for industrial application. Currently, continuously stirred tank reactors and one-stage processes are used principally for scale-up continuous fermentation of food waste. Electro-fermentation and iron-based or carbon-based additives can improve food waste fermentation, but their mechanisms and application need further investigation. After fermentation, the recovery of target products is a key problem due to the lack of green and economic methods. Precipitation, distillation, extraction, adsorption, and membrane separation can be considered, but the recovery step is still the most expensive in the entire treatment chain. It is expected to develop more efficient fermentation processes and recovery strategies based on food waste composition and market demand.
Electric Power System Transformations: A Review of Main Prospects and Challenges
Authors: Voropai Nikolai
Published: October 2020

The paper deals with the main prospects and challenges of radical transformations of electric power systems (EPSs) with changes in their structure and properties conditioned by wide use of innovative energy-related technologies and digitalization and intellectualization of system operation and control. Structural trends of EPS development are the focus of the analysis. Consideration is given to changes in EPS properties driven by the use of new technologies, to the problems of system flexibility and to its enhancement. EPS “resiliency” and “survivability” notions are subjected to comparison. The main factors favoring the formation of future EPSs to cyber-physical systems are discussed. Objective trends of EPS control and protection system development are under consideration.
A New Virtual Synchronous Generator Design Based on the SMES System for Frequency Stability of Low-Inertia Power Grids
Authors: Magdy Gaber , Bakeer Abualkasim , Nour Morsy , Petlenkov Eduard
Published: October 2020

In light of the challenges of integrating more renewable energy sources (RESs) into the utility grid, the virtual synchronous generator (VSG) will become an indispensable configuration of modern power systems. RESs are gradually replacing the conventional synchronous generators that are responsible for supplying the utility grid with the inertia damping properties, thus renewable power grids are more vulnerable to disruption than traditional power grids. Therefore, the VSG is presented to mimic the behavior of a real synchronous generator in the power grid through the virtual rotor concept (i.e., which emulates the properties of inertia and damping) and virtual primary and secondary controls (i.e., which emulate the conventional frequency control loops). However, inadequate imitation of the inertia power owing to the low and short-term power of the energy storage systems (ESSs) may cause system instability and fail dramatically. To overcome this issue, this paper proposes a VSG based on superconducting magnetic energy storage (SMES) technology to emulate the needed inertia power in a short time and thus stabilizing the system frequency at different disturbances. The proposed VSG based on SMES is applied to improve the frequency stability of a real hybrid power grid, Egyptian Power System (EPS), with high renewables penetration levels, nonlinearities, and uncertainties. The performance superiority of the proposed VSG-based SMES is validated by comparing it with the traditional VSG approach based on battery ESSs. The simulation results demonstrated that the proposed VSG based on the SMES system could significantly promote ultra-low-inertia renewable power systems for several contingencies.
Hybrid Energy Systems Sizing for the Colombian Context: A Genetic Algorithm and Particle Swarm Optimization Approach
Authors: Torres-Madroñero José Luis , Nieto-Londoño César , Sierra-Pérez Julián
Published: October 2020

The use of fossil resources for electricity production is one of the primary reasons for increasing greenhouse emissions and is a non-renewable resource. Therefore, the electricity generation by wind and solar resources have had greater applicability in recent years. Hybrid Renewable Energy Systems (HRES) integrates renewable sources and storage systems, increasing the reliability of generators. For the sizing of HRES, Artificial Intelligence (AI) methods such as Genetic Algorithms (GA) and Particle Swarm Optimization (PSO) stand out. This article presents the sizing of an HRES for the Colombian context, taking into account the energy consumption by three typical demands, four types of wind turbines, three types of solar panels, and a storage system for the system configuration. Two optimization approaches were set-up with both optimization strategies (i.e., GA and PSO). The first one implies the minimization of the Loss Power Supply Probability (LPSP). In contrast, the second one concerns adding the Total Annual Cost (TAC) or the Levelized Cost of Energy (LCOE) to the objective function. Results obtained show that HRES can supply the energy demand, where the PSO method gives configurations that are more adjusted to the considered electricity demands.
Sustainable Production of 5-Hydroxymethylfurfural from Pectin-Free Sugar Beet Pulp in a Simple Aqueous Phase System-Optimization with Doehlert Design
Authors: Pińkowska Hanna , Krzywonos Małgorzata , Wolak Paweł , Seruga Przemysław , Górniak Agata , Złocińska Adrianna , Ptak Michał
Published: October 2020

Waste solid residue from the hydrothermal extraction of pectin derived from sugar beet pulp was used as feedstock in the production of 5-hydroxymethylfurfural (5-HMF). The depolymerization of pectin-free sugar beet pulp (PF-SBP) to monosaccharides and their dehydration to 5-HMF were conducted in subcritical water using a batch reactor. The experimental design methodology was used in order to model the hydrothermal process and to optimize the operational parameters of the reaction, namely temperature and holding time. These parameters are required to achieve the highest yield of 5-HMF. The model predicts, in good agreement with experimental results (R2 = 0.935), an optimal yield of 5-HMF (of approximately 38% in relation to the cellulosic fraction content in the PF-SBP) at a temperature of 192.5 °C and a holding time of about 51.2 min. 5-HMF was successfully isolated from the reaction mixture using the liquid–liquid extraction method. The results are suitable for industrial upscaling and may become an incentive to introduce a new, environmentally friendly, uncomplicated, and efficient waste treatment method. The method would be used to treat products from the sugar refining industry, the treatment of which has proven to be problematic until now.
Reliability of PEA Measurement in Presence of an Air Void Defect
Authors: Imburgia Antonino , Romano Pietro , Rizzo Giuseppe , Viola Fabio , Ala Guido , Chen George
Published: October 2020

This paper deals with the reliability of the Pulsed Electro-Acoustic (PEA) technique in the case of a specimen containing an air layer. The first approach to this study has been proposed by the authors in previously published works. In these papers, the mathematical description, the PEA cell simulation model, and some experimental tests have been reported. In this work, a more accurate description of the acoustic wave behavior within the PEA cell and specimen with and without an air layer is given. Moreover, the comparison between simulation and experimental tests for both cases (specimen with and without air layer) allowed the validation of the previously developed PEA cell simulation model. The latter was previously validated only for a single layer specimen, here the good performances of the model have also been confirmed in the case of a multilayer specimen, also with an air layer. Experimental and simulation results show that the air layer acts as a barrier for the acoustic signal, due to the different acoustic impedance between the air and the solid dielectric material which constitute the specimen. Therefore, the aim of the present work is to demonstrate that in the case of a three-layers specimen, composed as dielectric-air-dielectric, the PEA cell is not able to provide the complete profile of the entire specimen under test.
Factors Influencing the Threats for Urban Energy Networks: The Inhabitants’ Point of View
Authors: Čábelková Inna , Strielkowski Wadim , Wende Frank-Detlef , Krayneva Raisa
Published: October 2020

Our paper focuses on eliciting the factors that constitute threats and dangers for urban energy networks, information networks, and energy grids in the cities. Moreover, we attempt to determine how these threats are perceived by the inhabitants of those cities. Urban energy networks tend to play an increasing role in achieving energy efficiency and environmental sustainability in large urban centers. Even though they do not necessarily correspond to reality, public opinions can substantially influence the adoption of relevant technologies in populous urban locations. We use the public opinion representative survey data from the Czech Republic (a sample of 1023 respondents) in order to show how people perceive the dangers and threats for urban energy networks from such events as: (i) Extensive and long-term power outage; (ii) service disruption of the Internet, mobile networks or telephone landlines; (iii) cyber-attacks, and (iv) the technological dependence of the state on multinational technological companies. Our results demonstrate that people who live in small rural settlements and medium-sized cities tend to be more afraid of the threats and dangers from urban energy networks such as electricity and Internet outages, compared to people residing in some smaller towns. As far as there would always be a fear of the new technologies and their vulnerabilities, the local governments, mass media, and Internet resources alike should pay more attention to providing the relevant and updated information on the threats to urban energy networks for the general public.
Enhanced Hydrate-Based Geological CO2 Capture and Sequestration as a Mitigation Strategy to Address Climate Change
Authors: Pandey Jyoti Shanker , Daas Yousef Jouljamal , Karcz Adam Paul , von Solms Nicolas
Published: October 2020

Geological sequestration of CO2-rich gas as a CO2 capture and storage technique has a lower technical and cost barrier compared to industrial scale-up. In this study, we have proposed CO2 capture and storage via hydrate in geological formation within the hydrate stability zone as a novel technique to contribute to global warming mitigation strategies, including carbon capture, utilization, and storage (CCUS) and to prevent vast methane release into the atmosphere caused by hydrate melting. We have attempted to enhance total gas uptake and CO2 capture efficiency in hydrate in the presence of kinetic promoters while using diluted CO2 gas (CO2-N2 mixture). Experiments are performed using unfrozen sands within hydrate stability zone condition and in the presence of low dosage surfactant and amino acids. Hydrate formation parameters, including sub-cooling temperature, induction time, total gas uptake, and split fraction, are calculated during the single-step formation and dissociation process. The effect of sands with varying particle sizes (160–630 µm, 1400–5000 µm), low dosage promoter (500–3000 ppm) and CO2 concentration in feed gas (20–30 mol%) on formation kinetic parameters was investigated. Enhanced formation kinetics are observed in the presence of surfactant (1000–3000 ppm) and hydrophobic amino acids (3000 ppm) at 120 bar and 1 ℃ experimental conditions. We report induction time in the range of 7–170 min and CO2 split fraction (0.60–0.90) in hydrate for 120 bar initial injection pressure. CO2 split fraction can be enhanced by reducing sand particle size or increasing the CO2 mol% in incoming feed gas at given injection pressure. This study also reports that formation kinetics in a porous medium are influenced by hydrate morphology. Hydrate morphology influences gas and water migration within sediments and controls pore space or particle surface correlation with the formation kinetics within coarse sediments. This investigation demonstrates the potential application of bio-friendly amino acids as promoters to enhance CO2 capture and storage within hydrate. Sufficient contact time at gas-liquid interface and higher CO2 separation efficiency is recorded in the presence of amino acids. The findings of this study could be useful in exploring the promoter-driven pore habitat of CO2-rich hydrates in sediments to address climate change.
Seed Removal Rates in Forest Remnants Respond to Forest Loss at the Landscape Scale
Authors: Hambuckers Alain , Trolliet Franck , Simon Astrid , Cazetta Eliana , Rocha-Santos Larissa
Published: October 2020

Seed removal is a key component of seed dispersal and may be influenced by both landscape-scale and local attributes, and it has been used as an indicator of the intensity of interactions between ecosystem components. We examined how the seed removal rates, which integrate the activity of seed dispersers and seed predators, vary with landscape-scale forest cover. We collected data under 34 trees belonging to two zoochoric species (Helicostylis tomentosa (Poepp. and Endl.) J. F. Macbr. and Inga vera Willd.) in 17 remnants in the Brazilian Atlantic forest, with different percentages of forest cover. The seed removal rate was estimated using a fast method based on the abundance of intact fruits and fruit scraps on the ground. The amount of forest cover affected the rate of seed removal in a humpbacked shape, with a maximum seed removal rate at intermediate forest cover. Seed removal rates must be related to the amount of food resources offered and diversity of dispersers and predators in the region. In landscapes with intermediate forest amount, there is a better balance between supply and demand for fruits, leading to a higher seed removal rate than more deforested or forested landscape. Our results also show that local factors, such as crop size and canopy surface, together with forest cover amount, are also important to the removal rate, depending on the species. In addition, our results showed that plant–animal interactions are occurring in all fragments, but the health status of these forests is similar to disturbed forests, even in sites immersed in forested landscapes.
Detecting Growth Phase Shifts Based on Leaf Trait Variation of a Canopy Dipterocarp Tree Species (Parashorea chinensis)
Authors: Deng Yun , Deng Xiaobao , Dong Jinlong , Zhang Wenfu , Hu Tao , Nakamura Akihiro , Song Xiaoyang , Fu Peili , Cao Min
Published: October 2020

Canopy species need to shift their adaptive strategy to acclimate to very different light environments as they grow from seedlings in the understory to adult trees in the canopy. However, research on how to quantitively detect ecological strategy shifts in plant ontogeny is scarce. In this study, we hypothesize that changes in light and tree height levels induce transitions in ecological strategies, and growth phases representing different adaptive strategies can be classified by leaf trait variation. We examined variations in leaf morphological and physiological traits across a vertical ambient light (represented by the transmittance of diffuse light, %TRANS) and tree height gradient in Parashorea chinensis, a large canopy tree species in tropical seasonal rainforest in Southwestern China. Multivariate regression trees (MRTs) were used to detect the split points in light and height gradients and classify ontogenetic phases. Linear piecewise regression and quadratic regression were used to detect the transition point in leaf trait responses to environmental variation and explain the shifts in growth phases and adaptive strategies. Five growth phases of P. chinensis were identified based on MRT results: (i) the vulnerable phase, with tree height at less than 8.3 m; (ii) the suppressed phase, with tree height between 8.3 and 14.9 m; (iii) the growth release phase, with tree height between 14.9 and 24.3 m; (iv) the canopy phase, with tree height between 24.3 and 60.9 m; and (v) the emergent phase, with tree height above 60.9 m. The suppressed phase and canopy phase represent “stress-tolerant” and “competitive” strategies, respectively. Light conditions drive the shift from the “stress-tolerant” to the “competitive” strategy. These findings help us to better understand the regeneration mechanisms of canopy species in forests.
Diversity Patterns Associated with Varying Dispersal Capabilities as a Function of Spatial and Local Environmental Variables in Small Wetlands in Forested Ecosystems
Authors: Tornwall Brett M. , Pitt Amber L. , Brown Bryan L. , Hawley-Howard Joanna , Baldwin Robert F.
Published: October 2020

The diversity of species on a landscape is a function of the relative contribution of diversity at local sites and species turnover between sites. Diversity partitioning refers to the relative contributions of alpha (local) and beta (species turnover) diversity to gamma (regional/landscape) diversity and can be influenced by the relationship between dispersal capability as well as spatial and local environmental variables. Ecological theory predicts that variation in the distribution of organisms that are strong dispersers will be less influenced by spatial properties such as topography and connectivity of a region and more associated with the local environment. In contrast, the distribution of organisms with limited dispersal capabilities is often dictated by their limited dispersal capabilities. Small and ephemeral wetlands are centers of biodiversity in forested ecosystems. We sampled 41 small and ephemeral wetlands in forested ecosystems six times over a two-year period to determine if three different taxonomic groups differ in patterns of biodiversity on the landscape and/or demonstrate contrasting relationships with local environmental and spatial variables. We focused on aquatic macroinvertebrates (aerial active dispersers consisting predominantly of the class Insecta), amphibians (terrestrial active dispersers), and zooplankton (passive dispersers). We hypothesized that increasing active dispersal capabilities would lead to decreased beta diversity and more influence of local environmental variables on community structure with less influence of spatial variables. Our results revealed that amphibians had very high beta diversity and low alpha diversity when compared to the other two groups. Additionally, aquatic macroinvertebrate community variation was best explained by local environmental variables, whereas amphibian community variation was best explained by spatial variables. Zooplankton did not display any significant relationships to the spatial or local environmental variables that we measured. Our results suggest that amphibians may be particularly vulnerable to losses of wetland habitat in forested ecosystems as they have high beta diversity. Consequently, the loss of individual small wetlands potentially results in local extirpations of amphibian species in forested ecosystems.
Faraday Waves in a Square Cell Network: The Effects of Varying the Cell Size
Authors: Peña-Polo Franklin , Carvajal-Mariscal Ignacio , Vargas Carlos A. , Sigalotti Leonardo Di G.
Published: October 2020

We have conducted experiments of the Faraday instability in a network of square cells filled with water for driving frequencies and amplitudes in the intervals 10≤F≤22 Hz and 0.1≤A≤3 mm, respectively. The experiments were aimed at studying the effects of varying the size of the cells on the surface wave patterns. Images of the surface wave patterns were recorded with a high-speed camera. The time series of photographs composing each video was Fourier analyzed, and information about the waveforms was obtained by using a Pearson correlation analysis. For small square cells of side length l=2.5 cm, adjacent cells collaborate synchronously to form regular patterns of liquid bumps over the entire grid, while ordered matrices of oscillons are formed at higher frequencies. As the size of the cells is increased to l=5 cm, collective cell behaviour at lower frequencies is no longer observed. As the frequency is increased, a transition from three triangularly arranged oscillons within each cell to three, or even four, irregularly arranged oscillons is observed. The wave patterns, the waveforms and the energy content necessary to excite Faraday waves are seen to depend on the cell size.
Childhood Leukemia in Small Geographical Areas and Proximity to Industrial Sources of Air Pollutants in Three Colombian Cities
Authors: Rodriguez-Villamizar Laura Andrea , Moreno-Corzo Feisar Enrique , Valbuena-Garcia Ana María , Uribe Pérez Claudia Janeth , Brome Bohórquez Mary Ruth , García García Héctor Iván , Bravo Luis Eduardo , Ortiz Martínez Rafael Gustavo , Niederbacher Velásquez Jürg , Osornio-Vargas Alvaro R.
Published: October 2020

Acute leukemia is the most common childhood cancer and has been associated with exposure to environmental carcinogens. This study aimed to identify clusters of acute childhood leukemia (ACL) cases and analyze their relationship with proximity to industrial sources of air pollution in three capital cities in Colombia during 2000–2015. Incident ACL cases were obtained from the population cancer registries for the cities of Bucaramanga, Cali, and Medellín. The inventory of industrial sources of emissions to the air was obtained from the regional environmental authorities and industrial conglomerates were identified. The Kulldorf’s circular scan test was used to detect city clusters and to identify clusters around industrial conglomerates. Multivariable spatial modeling assessed the effect of distance and direction from the industrial conglomerates controlling for socioeconomic status. We identified industrials sectors within a buffer of 1 km around industrial conglomerates related to the ACL clusters. Incidence rates showed geographical heterogeneity with low spatial autocorrelation within cities. The spatio-temporal tests identified one cluster in each city. The industries located within 1 km around the ACL clusters identified in the three cities represent different sectors. Exposure to air pollution from industrial sources might be contributing to the incidence of ACL cases in urban settings in Colombia.
The Relation between Domain-Specific Physical Behaviour and Cardiorespiratory Fitness: A Cross-Sectional Compositional Data Analysis on the Physical Activity Health Paradox Using Accelerometer-Assessed Data
Authors: Ketels Margo , Rasmussen Charlotte Lund , Korshøj Mette , Gupta Nidhi , De Bacquer Dirk , Holtermann Andreas , Clays Els
Published: October 2020

In contrast to leisure time physical activity (LTPA), occupational physical activity (OPA) does not have similar beneficial health effects. These differential health effects might be explained by dissimilar effects of LTPA and OPA on cardiorespiratory fitness (CRF). This study investigated cross-sectional associations between different physical behaviours during both work and leisure time and CRF by using a Compositional Data Analysis approach. Physical behaviours were assessed by two accelerometers among 309 workers with various manual jobs. During work time, more sedentary behaviour (SB) was associated with higher CRF when compared relatively to time spent on other work behaviours, while more SB during leisure time was associated with lower CRF when compared to other leisure time behaviours. Reallocating more time to moderate-to-vigorous physical activity (MVPA) from the other behaviours within leisure time was positively associated with CRF, which was not the case for MVPA during work. The results of our study are in line with the physical activity health paradox and we call for further study on the interaction between LTPA and OPA by implementing device-worn measures in a longitudinal design. Our results highlight the need for recommendations to take into account the different effects of OPA and LTPA on CRF.
Medial Branch Blocks for Diagnosis of Facet Joint Pain Etiology and Use in Chronic Pain Litigation
Authors: Lawson Gordon E. , Nolet Paul S. , Little Adam R. , Bhattacharyya Anit , Wang Vivian , Lawson C. Adam , Ko Gordon D.
Published: October 2020

A commonly disputed medicolegal issue is the documentation of the location, degree, and anatomical source of an injured plaintiff’s ongoing pain, particularly when the painful region is in or near the spine, and when the symptoms have arisen as result of a relatively low speed traffic crash. The purpose of our paper is to provide health and legal practitioners with strategies to identify the source of cervical pain and to aid triers of fact (decision makers) in reaching better informed conclusions. We review the medical evidence for the applications and reliability of cervical medial branch nerve blocks as an indication of painful spinal facets. We also present legal precedents for the legal admissibility of the results of such diagnostic testing as evidence of chronic spine pain after a traffic crash. Part of the reason for the dispute is the subjective nature of pain, and the fact that medical documentation of pain complaints relies primarily on the history given by the patient. A condition that can be documented objectively is chronic cervical spine facet joint pain, as demonstrated by medial branch block (injection). The diagnostic accuracy of medial branch blocks has been extensively described in the scientific medical literature, and evidence of facet blocks to objectively document chronic post-traumatic neck pain has been accepted as scientifically reliable in courts and tribunals in the USA, Canada and the United Kingdom. We conclude that there is convincing scientific medical evidence that the results of cervical facet blocks provide reliable objective evidence of chronic post-traumatic spine pain, suitable for presentation to an adjudicative decision maker.
Environmental Risk Factors for Early-Onset Alzheimer’s Dementia and Frontotemporal Dementia: A Case-Control Study in Northern Italy
Authors: Adani Giorgia , Filippini Tommaso , Garuti Caterina , Malavolti Marcella , Vinceti Giulia , Zamboni Giovanna , Tondelli Manuela , Galli Chiara , Costa Manuela , Vinceti Marco , Chiari Annalisa
Published: October 2020

Background: Early-onset dementia (EOD) is defined as dementia with symptom onset before 65 years. The role of environmental risk factors in the etiology of EOD is still undefined. We aimed at assessing the role of environmental risk factors in EOD etiology, taking into account its different clinical types. Methods: Using a case-control study, we recruited all EOD cases referred to Modena hospitals from 2016 to 2019, while the referent population was drawn from cases’ caregivers. We investigated residential history, occupational and environmental exposures to chemicals and lifestyle behaviors through a self-administered questionnaire. We computed the odds ratios of EOD risk (overall and restricting to the Alzheimer’s dementia (AD) or frontotemporal dementia (FTD) diagnoses) and the corresponding 95% confidence intervals using an unconditional logistic regression model. Results: Fifty-eight EOD patients (19 FTD and 32 AD) and 54 controls agreed to participate. Most of the investigated exposures, such as occupational exposure to aluminum, pesticides, dyes, paints or thinners, were associated with an increased odds ratio (OR) for FTD but not for AD. Long-term use of selenium-containing dietary supplements was associated with increased OR for EOD and, particularly, for FTD. For both EOD forms, smoking and playing football showed an increased odds ratio, while cycling was associated with increased risk only in FTD. Overall sports practice appeared to be a protective factor for both types. Conclusions: Our results suggest a role of environmental and behavioral risk factors such as some chemical exposures and professional sports in EOD etiology, in particular with reference to FTD. Overall sports practice may be associated with a reduced EOD risk.
Ciranda—An Inclusive Floor Seating Positioning System and Social Enterprise
Authors: Maximo Tulio , Foureaux Erika , Wang Xiao Lu , Fong Kenneth N. K.
Published: October 2020

One of the first challenges for many children with physical disabilities is to sit independently. A floor seating positioning system enables this milestone, helping a child to maintain eye level with other children, play and learn on the floor, rectify his or her posture, and, therefore, helps to include the child within his or her social spectrum. Ciranda is the first comprehensive floor seat solution in Brazil to attend to those needs. The project collected anthropometric data from 370 children who were unable to sit without support. A sample of 37 families of these children was visited, observed, and interviewed. A project requirement compiled key insights from the field data to support a multidisciplinary team of collaborators to co-design solutions. The project resulted in two floor seating positioning systems to attend to different needs. One is a social enterprise where the children’s parents and the community build the seat while the child in need and his or her friends engage in entertainment. The other is a salable seat that helps to raise funds for the social enterprise. The model also unravels other challenges common to assistive technologies, such as access to a device and training for the use and maintenance of the device.
Disparities between Rural and Urban Areas of the Central Region of Saudi Arabia in the Utilization and Time-Centeredness of Emergency Medical Services
Authors: Moafa Hassan N. , van Kuijk Sander Martijn Job , Alqahtani Dhafer M. , Moukhyer Mohammed E. , Haak Harm R.
Published: October 2020

The purpose of this study was to explore differences in characteristics of missions dispatched by Emergency Medical Services (EMS) between rural and urban areas of Riyadh province in Saudi Arabia (SA). It also aimed at identifying weaknesses related to utilization and Response Time (RT). The study retrospectively evaluated 146,639 completed missions in 2018 by measuring the utilization rate in rural and urban areas. The study shows there are six times more ambulance crews available for rural areas compared to urban. There were 22.1 missions per 1000 urban inhabitants and 11.2 missions per 1000 in rural areas. The median RT for high urgent trauma cases was 20.2 min in rural compared to 15.2 min in urban areas (p < 0.001). In urban areas, the median RT for high urgent medical cases was 16.1 min, while it was 15.2 min for high urgent trauma cases. Around 62.3% of emergency cases in urban and 56.5% in rural areas were responded to within 20.00 min. Women utilized EMS less frequently. The RT was increased in urban areas compared to previous studies. The RT in the central region of SA has been identified as equal, or less than 20.00 min in 62.4% of all emergency cases. To further improve adherence to the 20′ target, reorganizing the lowest urgent cases in the rural areas seems necessary.
Social Capital and Mental Health among Older Adults Living in Urban China in the Context of COVID-19 Pandemic
Authors: Sun Qian , Lu Nan
Published: October 2020

Although social capital has been found to be an important social determinant of mental health in later life, research on social capital in the context of COVID-19 and the interplay among subdimensions of social capital is lacking. The present study examined the mediating role of cognitive social capital on the relationship between structural social capital and mental health among older adults in urban China in the context of the COVID-19 pandemic. Data were collected from the Yangpu district in Shanghai, China, in July–August 2020. A quota sampling approach was used to recruit 472 respondents aged 60 years and older from 23 communities in the Yangpu district. Mental health was measured by depressive symptoms and life satisfaction. Cognitive social capital was assessed through trust and reciprocity, and structural social capital was assessed through organization memberships, and COVID-19 related volunteering and citizenship activity. Structural equation modeling was used to test the mediation model. The results show that cognitive social capital had a full mediation effect on the association between structural social capital and mental health indicators (life satisfaction: b = 0.122, SD = 0.029, p < 0.001; depressive symptoms: b = −0.343, SD = 0.119, p < 0.01). The findings indicate that social capital can play an important role in sustaining and improving mental health in the context of the COVID-19 pandemic. Policy and intervention implications are discussed.
Factors Associated with In-Hospital Mortality in Acute Care Hospital Settings: A Prospective Observational Study
Authors: Porcel-Gálvez Ana María , Barrientos-Trigo Sergio , Gil-García Eugenia , Aguilera-Castillo Olivia , Pérez-Fernández Antonio Juan , Fernández-García Elena
Published: October 2020

Background: In-hospital mortality is a key indicator of the quality of care. Studies so far have demonstrated the influence of patient and hospital-related factors on in-hospital mortality. Currently, new variables, such as nursing workload or the level of dependency, are being incorporated. We aimed to identify which individual, clinical and hospital characteristics are related to hospital mortality. Methods: A multicentre prospective observational study design was used. Sampling was conducted between February 2015 and October 2017. Patients over 16 years, admitted to medical or surgical units at 11 public hospitals in Andalusia (Spain), with a foreseeable stay of at least 48 h were included. Multivariate regression analyses were performed to analyse the data. Results: The sample consisted of 3821 assessments conducted in 1004 patients. The mean profile was that of a male (52%), mean age of 64.5 years old, admitted to a medical unit (56.5%), with an informal caregiver (60%). In-hospital mortality was 4%. The INICIARE (Inventario del Nivel de Cuidados Mediante Indicadores de Clasificación de Resultados de Enfermería) scale yielded an adjusted odds ratio [AOR] of 0.987 (95% confidence interval [CI]: 0.97–0.99) and the nurse staffing level (NSL) yielded an AOR of 1.197 (95% CI: 1.02–1.4). Conclusion: Nursing care dependency measured by INICIARE and nurse staffing level was associated with in-hospital mortality.
B Cell Activating Factor (BAFF) Is Required for the Development of Intra-Renal Tertiary Lymphoid Organs in Experimental Kidney Transplantation in Rats
Authors: Steines Louisa , Poth Helen , Herrmann Marlene , Schuster Antonia , Banas Bernhard , Bergler Tobias
Published: October 2020

Intra-renal tertiary lymphoid organs (TLOs) are associated with worsened outcome in kidney transplantation (Ktx). We used an anti-BAFF (B cell activating factor) intervention to investigate whether BAFF is required for TLO formation in a full MHC-mismatch Ktx model in rats. Rats received either therapeutic immunosuppression (no rejection, NR) or subtherapeutic immunosuppression (chronic rejection, CR) and were sacrificed on d56. One group additionally received an anti-BAFF antibody (CR + AB). Intra-renal T (CD3+) and B (CD20+) cells, their proliferation (Ki67+), and IgG+ plasma cells were analyzed by immunofluorescence microscopy. Formation of T and B cell zones and TLOs was assessed. Intra-renal expression of TLO-promoting factors, molecules of T:B crosstalk, and B cell differentiation was analyzed by qPCR. Intra-renal B and T cell zones and TLOs were detected in CR and were associated with elevated intra-renal mRNA expression of TLO-promoting factors, including CXCL13, CCL19, lymphotoxin-β, and BAFF. Intra-renal plasma cells were also elevated in CR. Anti-BAFF treatment significantly decreased intra-renal B cell zones and TLO, as well as intra-renal B cell-derived TLO-promoting factors and B cell differentiation markers. We conclude that BAFF-dependent intra-renal B cells promote TLO formation and advance local adaptive alloimmune responses in chronic rejection.
GPCR-Based Dopamine Sensors—A Detailed Guide to Inform Sensor Choice for In Vivo Imaging
Authors: Labouesse Marie A. , Cola Reto B. , Patriarchi Tommaso
Published: October 2020

Understanding how dopamine (DA) encodes behavior depends on technologies that can reliably monitor DA release in freely-behaving animals. Recently, red and green genetically encoded sensors for DA (dLight, GRAB-DA) were developed and now provide the ability to track release dynamics at a subsecond resolution, with submicromolar affinity and high molecular specificity. Combined with rapid developments in in vivo imaging, these sensors have the potential to transform the field of DA sensing and DA-based drug discovery. When implementing these tools in the laboratory, it is important to consider there is not a ‘one-size-fits-all’ sensor. Sensor properties, most importantly their affinity and dynamic range, must be carefully chosen to match local DA levels. Molecular specificity, sensor kinetics, spectral properties, brightness, sensor scaffold and pharmacology can further influence sensor choice depending on the experimental question. In this review, we use DA as an example; we briefly summarize old and new techniques to monitor DA release, including DA biosensors. We then outline a map of DA heterogeneity across the brain and provide a guide for optimal sensor choice and implementation based on local DA levels and other experimental parameters. Altogether this review should act as a tool to guide DA sensor choice for end-users.
Contribution of Autophagy-Notch1-Mediated NLRP3 Inflammasome Activation to Chronic Inflammation and Fibrosis in Keloid Fibroblasts
Authors: Lee Seongju , Kim Sun Kyeon , Park Hyungsun , Lee Yu Jin , Park Song Hee , Lee Kyung Jae , Lee Dong Geon , Kang Hoon , Kim Jung Eun
Published: October 2020

Keloid is a representative chronic fibroproliferative condition that occurs after tissue injury. Emerging evidence showed that activation of NACHT, LRR, and PYD domains-containing protein 3 (NLRP3) inflammasome is involved in the pro-inflammatory response in injured tissues. However, the role of NLRP3 inflammasome in keloid progression remains unclear. Notch signaling, which activates NLRP3 inflammasome, is known to contribute to scar formation in keloid, but the cause of enhanced Notch signaling in keloid is not clear. We sought to investigate whether autophagy regulates Notch1 signaling in keloid fibroblasts and determine whether Notch1 signaling might regulate NLRP3 inflammasomes and myofibroblast differentiation. An in vitro model of keloid was established by culturing primary keloid fibroblasts from patients. Expression levels of Notch1, NLRP3 inflammasome proteins, pro-inflammatory cytokines, and myofibroblast markers in keloid fibroblasts were examined and compared with those in normal fibroblasts. Autophagy known to mediate Notch1 degradation was also monitored in fibroblasts. Small interfering RNA (siRNA) targeting Notch1 was used to transfect keloid fibroblasts to further examine the role of Notch signaling in NLRP3 inflammasome activation. Expression levels of Notch1 and NLRP3 inflammasome in keloid fibroblasts increased compared to those in normal fibroblasts. Such increases were accompanied by increased LC3 levels and reduced autophagic flux. Notch1 silencing in keloid fibroblasts by siRNA transfection significantly suppressed increased levels of overall NLRP3 inflammasome complex proteins, NF-kB, and α-smooth muscle actin. Autophagy induction by rapamycin treatment in keloid fibroblasts effectively suppressed expression levels of Notch1 and NLRP3 inflammasome proteins. Decreased autophagy activity in keloid can result in Notch1-mediated myofibroblast activation and NLRP3 inflammasome signaling activation which is critical for chronic inflammation. Collectively, these results identify Notch1 as a novel activator of NLRP3 inflammasome signaling leading to chronic tissue damage and myofibroblast differentiation in keloid progression.
Current Insights in Elucidation of Possible Molecular Mechanisms of the Juvenile Form of Batten Disease
Authors: Shematorova Elena K. , Shpakovski George V.
Published: October 2020

The neuronal ceroid lipofuscinoses (NCLs) collectively constitute one of the most common forms of inherited childhood-onset neurodegenerative disorders. They form a heterogeneous group of incurable lysosomal storage diseases that lead to blindness, motor deterioration, epilepsy, and dementia. Traditionally the NCL diseases were classified according to the age of disease onset (infantile, late-infantile, juvenile, and adult forms), with at least 13 different NCL varieties having been described at present. The current review focuses on classic juvenile NCL (JNCL) or the so-called Batten (Batten-Spielmeyer-Vogt; Spielmeyer-Sjogren) disease, which represents the most common and the most studied form of NCL, and is caused by mutations in the CLN3 gene located on human chromosome 16. Most JNCL patients carry the same 1.02-kb deletion in this gene, encoding an unusual transmembrane protein, CLN3, or battenin. Accordingly, the names CLN3-related neuronal ceroid lipofuscinosis or CLN3-disease sometimes have been used for this malady. Despite excessive in vitro and in vivo studies, the precise functions of the CLN3 protein and the JNCL disease mechanisms remain elusive and are the main subject of this review. Although the CLN3 gene is highly conserved in evolution of all mammalian species, detailed analysis of recent genomic and transcriptomic data indicates the presence of human-specific features of its expression, which are also under discussion. The main recorded to date changes in cell metabolism, to some extent contributing to the emergence and progression of JNCL disease, and human-specific molecular features of CLN3 gene expression are summarized and critically discussed with an emphasis on the possible molecular mechanisms of the malady appearance and progression.
The Dual Role of Myeloperoxidase in Immune Response
Authors: Arnhold Jürgen
Published: October 2020

The heme protein myeloperoxidase (MPO) is a major constituent of neutrophils. As a key mediator of the innate immune system, neutrophils are rapidly recruited to inflammatory sites, where they recognize, phagocytose, and inactivate foreign microorganisms. In the newly formed phagosomes, MPO is involved in the creation and maintenance of an alkaline milieu, which is optimal in combatting microbes. Myeloperoxidase is also a key component in neutrophil extracellular traps. These helpful properties are contrasted by the release of MPO and other neutrophil constituents from necrotic cells or as a result of frustrated phagocytosis. Although MPO is inactivated by the plasma protein ceruloplasmin, it can interact with negatively charged components of serum and the extracellular matrix. In cardiovascular diseases and many other disease scenarios, active MPO and MPO-modified targets are present in atherosclerotic lesions and other disease-specific locations. This implies an involvement of neutrophils, MPO, and other neutrophil products in pathogenesis mechanisms. This review critically reflects on the beneficial and harmful functions of MPO against the background of immune response.