bluuebunny/crossref_metadata_2025 huggingface dataset:
Protocol: Can coronary artery calcium score identified on thoracic planning CT scans be used and actioned to identify cancer survivors at high risk of cardiac events: A feasibility study in cancer survivors undergoing radiotherapy in Australia
Authors: Dalla Via Jack , Stewart Nina , Kennedy Mary A , Cehic Daniel A , Purnell Peter , Toohey Joanne , Morton Jamie , Ramchand Sabashini K , Lewis Joshua R , Zissiadis Yvonne
Published: July 2023

Introduction

A coronary artery calcium (CAC) CT scan can identify calcified plaque and predict risk of future cardiac events. Cancer survivors undergoing thoracic radiotherapy routinely undergo a planning CT scan, which presents a unique opportunity to use already obtained medical imaging to identify those at the highest risk of cardiac events. While radiation therapy is an important modality for many cancer treatments, radiation dose to the heart in thoracic radiotherapy leads to cardiotoxicity and may accelerate pre-existing atherosclerosis. The primary aims of this study are to investigate the feasibility of using CAC scores calculated on thoracic radiotherapy planning CT scans to identify a subset of cancer survivors at an increased risk of future cardiac events, and to establish and evaluate a referral pathway for assessment and management in a cardio-oncology clinic. An optional substudy aims to investigate using abdominal aortic calcification (AAC) as a practical, low-radiation alternative to CAC to evaluate and monitor vascular health.

Methods and analysis

This is an observational, prospective study in a minimum of 100 cancer survivors commencing radiotherapy. Participants will have CAC scored from thoracic radiotherapy planning CT scans. Those identified as high risk (CAC score>0) will be referred to a cardio-oncology clinic. Feasibility, determined by adherence to the recommended pathway, and impact on quality of life and anxiety measured via questionnaire, will be assessed. Participants in Western Australia will be invited to participate in a 12-month observational pilot substudy, investigating lifestyle behaviours and the use of a dual-energy X-ray absorptiometry machine to measure musculoskeletal health and AAC.

Ethics and dissemination

Ethics approval has been obtained from St Vincent’s Hospital, Sydney (Project number 2021/ETH11847), GenesisCare and Edith Cowan University (2022-03326-DALLAVIA). Study results will be reported in peer-reviewed academic journals, at scientific conferences, and at clinical forums, irrespective of the results observed.

Trial registration number

ACTRN12621001343897.
Association of follicle-stimulating hormone with lipid profiles in older women: a cross-sectional SPECT-China study
Authors: Zhang Wen , Chen Yingchao , Zhang Xian , Chen Yi , Han Bing , Li Qin , Xia Fangzhen , Zhai Hualing , Wang Ningjian , Lu Yingli
Published: July 2023

Aims

Follicle-stimulating hormone (FSH) is associated with higher risks of metabolic syndrome and diabetes in menopausal women. We aimed to investigate whether FSH was associated with the lipid profile in women older than 55 years.

Design

The data were obtained from a cross-sectional study.

Participants

Our data were from the Survey on Prevalence in East China for Metabolic Diseases and Risk Factors (China, including Shanghai and Zhejiang, Jiangxi and Anhui provinces). A total of 1795 women older than 55 years were selected.

Methods

Morning serum sex hormones and lipid profiles were measured. Linear and logistic regression analyses were used to analyse the data.

Results

Lower FSH was associated with lower high-density lipoprotein cholesterol (HDL-C) and higher triglycerides (TG), total cholesterol (TC)/HDL-C ratio and low-density lipoprotein cholesterol (LDL-C)/HDL-C ratio (all p for trend <0.05) after adjusting for age and other sex hormones. After further adjustment for body mass index, diabetes and hypertension, the associations of FSH with the lipid profile weakened, but the associations of FSH quartiles with HDL-C and the TC/HDL-C ratio were still significant (both p for trend <0.05). Compared with women in the highest FSH quartile, the odds of low HDL-C (HDL-C<1.04 mmol/L) in women in the lowest FSH quartile were 5.25 (95% CI 1.60 to 17.26) (p for trend <0.05) in the fully adjusted model, and the odds of TC≥6.22 mmol/L, TGs≥2.26 mmol/L and LDL-C≥4.14 mmol/L were not significant. Luteinising hormone did not show a significant association with dyslipidaemia.

Conclusion

Lower FSH was associated with a worse lipid profile in women older than 55. Diabetes, adiposity and hypertension mostly explained the association of FSH with TGs and the LDL-C/HDL-C ratio but only partially explained the associations of FSH with HDL-C and the TC/HDL-C ratio.
Quality assessment of clinical practice guidelines in Kenya using the AGREE II tool: a methodological review
Authors: Sagam Caleb Kimutai , Were Lisa M , Otieno Jenifer A , Mulaku Mercy N , Kariuki Simon , Ochodo Eleanor
Published: July 2023

Objective

To assess the quality of available and accessible national Clinical Practice Guidelines (CPGs) in Kenya using the Appraisal of Guidelines for Research and Evaluation II (AGREE II) tool.

Methods

We searched the websites of the Kenyan Ministry of Health, professional associations and contacted experts in relevant organisations. Our scope was guidelines on maternal, neonatal, nutritional disorders, injuries, communicable and non-communicable diseases in Kenya published in the last 5 years until 30 June 2022. Study selection and data extraction were done by three independent reviewers with disagreements resolved via discussion or with a senior reviewer. We conducted a quality assessment using the online English version of AGREE II tool across six domains. Descriptive statistics were analysed using Stata software V.17. The primary outcome was the methodological quality of the included CPGs assessed by the AGREE II tool score.

Results

We retrieved 95 CPGs and included 24 in the analysis after screening for eligibility. The CPGs scored best in clarity of presentation and least in the rigour of development. In descending order, the appraisal scores (mean and CI) per domain were as follows: Clarity of presentation 82.96% (95% CI 78.35% to 87.57%) with all guidelines scoring above 50%. Scope and purpose 61.75% (95% CI 54.19% to 69.31%) with seven guidelines scoring less than 50%. Stakeholder involvement 45.25% (95% CI 40.01% to 50.49%) with 16 CPGs scoring less than 50%. Applicability domain 19.88% (95% CI 13.32% to 26.43%) with only one CPG scoring above 50%. Editorial independence 6.92% (95% CI 3.47% to 10.37%) with no CPG scoring above 50% and rigour of development 3% (95% CI 0.61% to 5.39%) with no CPG scoring at least 50%.

Conclusion

Our findings suggest that the quality of CPGs in Kenya is limited mainly by the rigour of development, editorial independence, applicability and stakeholder involvement. Training initiatives on evidence-based methodology among guideline developers are needed to improve the overall quality of CPGs for better patient care.
Physician engagement in organisational patient safety through the implementation of a Medical Safety Huddle initiative: a qualitative study
Authors: Rotteau Leahora , Othman Dalia , Dunbar-Yaffe Richard , Fortin Chris , Go Katharyn , Mayo Amanda , Pelc Jordan , Wolfstadt Jesse , Guo Meiqi , Soong Christine
Published: July 2023

Background

Efforts to increase physician engagement in quality and safety are most often approached from an organisational or administrative perspective. Given hospital-based physicians’ strong professional identification, physician-led strategies may offer a novel strategic approach to enhancing physician engagement. It remains unclear what role medical leadership can play in leading programmes to enhance physician engagement. In this study, we explore physicians’ experience of participating in a Medical Safety Huddle initiative and how participation influences engagement with organisational quality and safety efforts.

Methods

We conducted a qualitative study of the Medical Safety Huddle initiative implemented across six sites. The initiative consisted of short, physician focused and led, weekly meetings aimed at reviewing, anticipating and addressing patient safety issues. We conducted 29 semistructured interviews with leaders and participants. We applied an interpretive thematic analysis to the data using self-determination theory as an analytic lens.

Results

The results of the thematic analysis are organised in two themes, (1) relatedness and meaningfulness, and (2) progress and autonomy, representing two forms of intrinsic motivation for engagement that we found were leveraged through participation in the initiative. First, participation enabled a sense of community and a ‘safe space’ in which professionally relevant safety issues are discussed. Second, participation in the initiative created a growing sense of ability to have input in one’s work environment. However, limited collaboration with other professional groups around patient safety and the ability to consistently address reported concerns highlights the need for leadership and organisational support for physician engagement.

Conclusion

The Medical Safety Huddle initiative supports physician engagement in quality and safety through intrinsic motivation. However, the huddles’ implementation must align with the organisation’s multipronged patient safety agenda to support multidisciplinary collaborative quality and safety efforts and leaders must ensure mechanisms to consistently address reported safety concerns for sustained physician engagement.
An efficient strategy for evaluating new non-invasive screening tests for colorectal cancer: the guiding principles
Authors: Bresalier Robert S , Senore Carlo , Young Graeme P , Allison James , Benamouzig Robert , Benton Sally , Bossuyt Patrick M M , Caro Luis , Carvalho Beatriz , Chiu Han-Mo , Coupé Veerle M H , de Klaver Willemijn , de Klerk Clasine Maria , Dekker Evelien , Dolwani Sunil , Fraser Callum G , Grady William , Guittet Lydia , Gupta Samir , Halloran Stephen P , Haug Ulrike , Hoff Geir , Itzkowitz Steven , Kortlever Tim , Koulaouzidis Anastasios , Ladabaum Uri , Lauby-Secretan Beatrice , Leja Mārcis , Levin Bernard , Levin Theodore Robert , Macrae Finlay , Meijer Gerrit A , Melson Joshua , O'Morain Colm , Parry Susan , Rabeneck Linda , Ransohoff David F , Sáenz Roque , Saito Hiroshi , Sanduleanu-Dascalescu Silvia , Schoen Robert E , Selby Kevin , Singh Harminder , Steele Robert J C , Sung Joseph J Y , Symonds Erin Leigh , Winawer Sidney J
Published: July 2023

Objective

New screening tests for colorectal cancer (CRC) are rapidly emerging. Conducting trials with mortality reduction as the end point supporting their adoption is challenging. We re-examined the principles underlying evaluation of new non-invasive tests in view of technological developments and identification of new biomarkers.

Design

A formal consensus approach involving a multidisciplinary expert panel revised eight previously established principles.

Results

Twelve newly stated principles emerged. Effectiveness of a new test can be evaluated by comparison with a proven comparator non-invasive test. The faecal immunochemical test is now considered the appropriate comparator, while colonoscopy remains the diagnostic standard. For a new test to be able to meet differing screening goals and regulatory requirements, flexibility to adjust its positivity threshold is desirable. A rigorous and efficient four-phased approach is proposed, commencing with small studies assessing the test’s ability to discriminate between CRC and non-cancer states (phase I), followed by prospective estimation of accuracy across the continuum of neoplastic lesions in neoplasia-enriched populations (phase II). If these show promise, a provisional test positivity threshold is set before evaluation in typical screening populations.Phase IIIprospective studies determine single round intention-to-screen programme outcomes and confirm the test positivity threshold.Phase IVstudies involve evaluation over repeated screening rounds with monitoring for missed lesions. Phases III and IV findings will provide the real-world data required to model test impact on CRC mortality and incidence.

Conclusion

New non-invasive tests can be efficiently evaluated by a rigorous phased comparative approach, generating data from unbiased populations that inform predictions of their health impact.
Artificial intelligence (AI) molecular analysis tool assists in rapid treatment decision in lung cancer: a case report
Authors: Waissengrin Barliz , Garasimov Alexandra , Bainhoren Or , Merimsky Ofer , Shamai Sivan , Erental Ariel , Wolf Ido , Hershkovitz Dov
Published: July 2023

Leptomeningeal involvement among non-small cell lung cancer (NSCLC) patients is an aggressive form of disease that requires quick and efficient treatment. In this case report, we describe a woman in her 40s with a presenting symptom of headache that ultimately was diagnosed as leptomeningeal spread from NSCLC adenocarcinoma. We identified EGFR mutation in less than 48 hours from the biopsy using imagene-artificial intelligence’s real-time algorithmic solution on the pathological diagnostic slide.
Using administrative data to evaluate national policy impacts on child and maternal health: a research framework from the Maternal and Child Health Network (MatCHNet)
Authors: Stewart Emma , Brophy Sinead , Cookson Richard , Gilbert Ruth , Given Joanne , Hardelid Pia , Harron Katie , Leyland Alastair , Pearce Anna , Wood Rachael , Dundas Ruth
Published: July 2023

Reducing health inequalities by addressing the social circumstances in which children are conceived and raised is a societal priority. Early interventions are key to improving outcomes in childhood and long-term into adulthood. Across the UK nations, there is strong political commitment to invest in the early years. National policy interventions aim to tackle health inequalities and deliver health equity for all children. Evidence to determine the effectiveness of socio-structural policies on child health outcomes is especially pressing given the current social and economic challenges facing policy-makers and families with children. As an alternative to clinical trials or evaluating local interventions, we propose a research framework that supports evaluating the impact of whole country policies on child health outcomes. Three key research challenges must be addressed to enable such evaluations and improve policy for child health: (1) policy prioritisation, (2) identification of comparable data and (3) application of robust methods.
Clinical phenotype and genetic function analysis of a rare family with hereditary leiomyomatosis and renal cell carcinoma complicated with Birt–Hogg–Dubé syndrome
Authors: Pan Hong-Hong , Ruan Dan-Dan , Wu Min , Chen Ting , Lu Tao , Gan Yu-Mian , Wang Chen , Liao Li-Sheng , Lin Xin-Fu , Chen Xin , Zhu Yao-Bin , Fang Zhu-Ting , Yu Qing-Hua , Yang Guo-Kai , Ye Lie-Fu , Luo Jie-Wei
Published: July 2023

To date, over 200 families with hereditary leiomyomatosis and renal cell carcinoma (HLRCC) and over 600 families with Birt–Hogg–Dubé (BHD) syndrome have been reported, with low incidence. Here, we describe a patient with suspected rare HLRCC complicated by BHD syndrome. The proband (II1) had characteristic cutaneous leiomyoma-like protrusions on the neck and back, a left renal mass and multiple right renal, liver and bilateral lung cysts. Three family members (I1, II2, II3) had a history of renal cancer and several of the aforementioned clinical features. Two family members (II1, II3) diagnosed with fumarate hydratase (FH)-deficient papillary RCC via pathological biopsy carried two heterozygous variants:FH(NM_000143.3) missense mutation c.1189G>A (p.Gly397Arg) andFLCN(NM_144997.5) frameshift mutation c.1579_1580insA (p.Arg527Glnfs*75). No family member carrying a single variant had renal tumours. In HEK293T cells transfected with mutant vectors, mRNA and protein expression afterFLCNp.Arg527Glnfs*75 andFHp.Gly397Arg mutations were significantly lower than those in wild-type (WT) cells. Cell immunofluorescence showed altered protein localisation and reduced protein expression afterFLCNp.Arg527Glnfs*75 mutation. The FH WT was uniformly distributed in the cytoplasm, whereas FH protein expression was reduced after the p.Gly397Arg mutation and scattered sporadically with altered cell localisation. Patients with two variants may have a significantly increased penetrance of RCC.
Influence of cardiorespiratory fitness and MRI measures of neuroinflammation on hippocampal volume in multiple sclerosis
Authors: Morozumi Tetsu , Preziosa Paolo , Meani Alessandro , Albergoni Matteo , Margoni Monica , Pagani Elisabetta , Filippi Massimo , Rocca Maria A
Published: July 2023

Background

The hippocampus is a clinically relevant region where neurogenesis and neuroplasticity occur throughout the whole lifespan. Neuroinflammation and cardiorespiratory fitness (CRF) may influence hippocampal integrity by modulating the processes promoting neurogenesis and neuroprotection that contribute to the preservation of functions. This study aimed to investigate the effects of neuroinflammation and CRF on hippocampal volume in multiple sclerosis (MS) patients with relapsing-remitting (RR) and progressive (P) clinical phenotypes. The influence of neuroinflammation and CRF on brain, grey matter (GM) and thalamic volumes was also assessed to determine whether the effects were specific for the hippocampus.

Method

Brain 3T structural MRI scans and maximum oxygen consumption (VO2max), a proxy of CRF, were acquired from 81 MS patients (27 RR and 54 P) and 45 age-matched and sex-matched healthy controls. T2-hyperintense white matter lesion volume (T2-LV) and choroid plexuses volume (CPV) were quantified as neuroinflammatory measures. Associations of demographic, clinical, neuroinflammatory and CRF measures with normalised brain, GM, hippocampal and thalamic volumes in relapsing-remitting MS (RRMS) and progressive MS patients were assessed using Shapley and best subset selection regression.

Results

For most volumetric measures, the largest portions of variance were explained by T2-LV (variable importance (VI)=9.4–39.4) and CPV (VI=4.5–26.2). VO2max explained the largest portion of variance of normalised hippocampal volume only in RRMS patients (VI=16.9) and was retained as relevant predictor (standardised β=0.374, p=0.023) with T2-LV (standardised β=−0.330, p=0.016).

Conclusions

A higher CRF may play a specific neuroprotective role on MS patients’ hippocampal integrity, but only in the RR phase of the disease.
Multi-criteria decision analysis in assessing watershed scale pollution risk: a review of combined approaches and applications
Authors: Akdogan Zeynep , Guven Basak
Published: July 2023

Decision-making tools have become a prominent methodology in watershed management for many years due to the complexity of environmental systems and requirement for multi-disciplinary expertise. Multi-Criteria Decision Analysis (MCDA) is a systematic methodology, which combines hierarchical structures of a problem and priorities for the alternatives in many fields. This study reviews MCDA applications in pollution risk assessment in the abiotic environments of watersheds for multi-pollutants. Over 80 papers published between 2000 and 2021 are identified in three categories of the Web of Science Core Collection database: “Environmental Sciences”, “Environmental Studies”, and “Water Resources”. The publications are further classified according to different environmental compartments: surface water, groundwater, and soil to investigate MCDA applications in these matrices. Finally, the distribution of the publications according to contaminants and MCDA methods used are also examined. The results reveal that the number of the studies focusing on pollution risk assessment within watersheds has been significantly increasing, especially over the last decade. However, there are still limited MCDA applications linking environmental compartments. Despite several MCDA studies focusing on the vulnerability of watersheds to multiple pollutants, studies related with emerging pollutants are scarce. Moreover, compared to non-point source pollution, studies adopting MCDA to investigate pollutant concentrations coming from point sources are relatively few. According to the overall distributions of MCDA methods, Analytic Hierarchy Process, a commonly found method in the literature that adopts a technique of pairwise comparison to prioritize criteria of prominence, dominates 34% of the publications.
Running vacuum in QFT in FLRW spacetime: the dynamics of $$\rho _{\textrm{vac}}(H)$$ from the quantized matter fields
Authors: Moreno-Pulido Cristian , Peracaula Joan Solà , Cheraghchi Samira
Published: July 2023

Phenomenological work in the last few years has provided significant support to the idea that the vacuum energy density (VED) is a running quantity with the cosmological evolution and that this running helps to alleviate the cosmological tensions afflicting the $$\Lambda $$
Λ
CDM. On the theoretical side, recent devoted studies have shown that the properly renormalized $$\rho _{\textrm{vac}}$$

ρ
vac

in QFT in FLRW spacetime adopts the ‘running vacuum model’ (RVM) form. While in three previous studies by two of us (CMP and JSP) such computations focused solely on scalar fields non-minimally coupled to gravity, in the present work we compute the spin-1/2 fermionic contributions and combine them both. The calculation is performed using a new version of the adiabatic renormalization procedure based on subtracting the UV divergences at an off-shell renormalization point M. The quantum scaling of $$\rho _{\textrm{vac}}$$

ρ
vac

with M turns into cosmic evolution with the Hubble rate, H. As a result the ‘cosmological constant’ $$\Lambda $$
Λ
appears in our framework as the nearly sustained value of $$8\pi G(H)\rho _{\textrm{vac}}(H)$$

8
π
G

(
H
)

ρ
vac

(
H
)

around (any) given epoch H, where G(H) is the gravitational coupling, which is also running, although very mildly (logarithmically). We find that the VED evolution at present reads $$\delta \rho _\textrm{vac}(H)\sim \nu _{\textrm{eff}}\, m_{\textrm{Pl}}^2 \left( H^2-H_0^2 \right) \ (|\nu _{\textrm{eff}}|\ll 1)$$

δ

ρ
vac

(
H
)

∼

ν
eff

m

Pl

2

H
2

-

H
0
2

(
|

ν
eff

|
≪
1
)

. The coefficient $$\nu _{\textrm{eff}}$$

ν
eff

receives contributions from all the quantized fields, bosons and fermions, which we compute here for an arbitrary number of matter fields. Remarkably, there also exist higher powers $$\mathcal{O}(H^{6})$$

O
(

H
6

)

which can trigger inflation in the early universe. Finally, the equation of state (EoS) of the vacuum receives also quantum corrections from bosons and fermion fields, shifting its value from − 1. The striking consequence is that the EoS of the quantum vacuum may nowadays effectively appears as quintessence.
Research on the Construction of Financial Computing Model Based on BSDE Algorithm
Authors: Cai Youli
Published: July 2023

Economic and social development has made financial engineering an increasingly important research area, and more and more financial problems cannot be solved directly by analytical formulas. In view of this, algorithms that apply computer technology to financial engineering have emerged. In this study, the Backward Stochastic Differential Equation (BSDE) algorithm is used to investigate and analyse the problem of option pricing calculation in finance. In the research process, GBSDE-Theta parallel algorithm composed of BSDE-Theta algorithm and GPU algorithm uses the new algorithm to establish a computing model in the financial engineering field, which applies to the calculation of enterprise option pricing. The research results show that compared with the basic algorithm, the actual option values of the option pricing data obtained by using the GBSDE-Theta parallel algorithm are more closely matched. The computational model can achieve a speedup ratio of about 230 times of the serial version with the number of time steps [Formula: see text] and the number of simulated paths 80,000. About the relative error of the GBSDE-Theta algorithm, there are 80 points within 3% and only 16 points over 3.00%, which is a relatively small error. The above results show that the financial computing system obtained in this study is highly feasible and effective, and can provide a new research idea for the progress and development of other computations in the financial field.
Query-by-Example Spoken Term Detection for Zero-Resource Languages Using Heuristic Search
Authors: P Sudhakar , K Sreenivasa Rao , Mitra Pabitra
Published: July 2023

Query-by-Example spoken content retrieval is a demanding and challenging task when a large volume of spoken content is piled up in the repositories without annotation. In the absence of annotation, spoken content retrieval is achieved by capturing the similarities between the query and spoken terms from the acoustic feature representation itself. Dynamic Time Warping (DTW) centric techniques identify the optimal alignment between the acoustic feature representations and capture the similarities between query and spoken terms. Despite feasibility, the DTW-centric techniques produce a lot of false alarms due to the variabilities that exist in natural speech and degrade the performance. In the proposed approach, the variability challenges are addressed in two stages. At first, the speaker-independent acoustic feature representation was obtained from the deep convolutional neural networks that reduce the speaker variabilities. In the second stage, the similarities between the query and spoken term were captured using the heuristic search method. The proposed approach was compared with other state-of-the-art methods using Microsoft Low-Resource Language speech corpus. A 3% improvement and 32% reduction in the hit and false alarm ratio were achieved across languages.
Nth Composite Iterative Scheme via Weak Contractions with Application
Authors: Qawasmeh Tariq , Bataihah Anwar , Bataihah Khalaf , Qazza Ahmad , Hatamleh Raed
Published: July 2023

The main goal of this study is to formulate an effective iterative scheme, namely, an nth− composite iterative scheme for approximating the fixed point of a self‐map with weak contraction property. We show that the nth− composite iterative scheme is faster than the scheme obtained by Sintunavarat–Pitea’s iterative scheme. We present some examples using the MATLAB simulator to illustrate our results. Finally, we approximate the solution of some integral equations using our scheme and the Sintunavarat–Pitea scheme.
Replacement Effect of Fish Meal by Plant Protein Sources in Olive Flounder (Paralichthys olivaceus) Feeds with an Addition of Jack Mackerel Meal on Growth, Feed Availability, and Biochemical Composition
Authors: Baek Seong Il , Jeong Hae Seung , Cho Sung Hwoan
Published: July 2023

Application of feed stimulants is very helpful to increase the feed intake of fish, especially in the development of low fish meal (FM) diets. FM replacement effect by various plant protein sources (corn gluten meal (CGM), soy protein concentrate (SPC), and corn protein concentrate (CPC)) in diets with an addition of jack mackerel meal (JMM) as feed stimulants on growth, feed availability, and biochemical composition of olive flounder was elucidated. An experimental design of two-way (two replacement levels (25% and 50%) × 3 replacement sources (CGM, SPC, and CPC)) analysis of variance was adopted. Seven diets were formulated. Amount of 60% FM was contained in the control (Con) diet. In the Con diet, 25% and 50% FM were replaced by CGM, SPC, and CPC with an addition of 12% JMM as feed stimulants, referred to as the CGM25, CGM50, SPC25, SPC50, CPC25, and CPC50 diets, respectively. Four hundred and twenty juvenile fish were distributed into 21 flow-through tanks. All diets were assigned to triplicate groups of fish. Fish were hand-fed to satiation twice a day for 56 days. Both dietary replacement levels and sources had statistical effect on weight gain (

P
<
0.0001

and

P
<
0.045

, respectively), specific growth rate (SGR) (

P
<
0.0001

and

P
<
0.033

), and feed consumption (

P
<
0.0001

and

P
<
0.03

) of fish. Dietary increased FM replacement levels lowered weight gain, SGR, and feed consumption of fish. Weight gain, SGR, and feed consumption of fish fed the Con and CGM25 diets were statistically (

P
<
0.05

) greater than those of fish fed the CGM50, SPC50, and CPC50 diets. Both replacement level and source had no statistical effect on feed utilization, biochemical composition except for statistical effect of replacement source on glycine content of fish, and lysozyme and superoxide dismutase (SOD) activities of fish. FM up to 25% could be substituted with CGM, SPC, and CPC in the olive flounder feeds supplemented with 12% JMM as feed stimulants without compromising growth, feed utilization, and lysozyme and SOD activities.
Data from Results of an Open-label, Phase 1a/b Study of Pembrolizumab plus Olaratumab in Patients with Unresectable, Locally Advanced or Metastatic Soft Tissue Sarcoma
Authors: Schöffski Patrick , Bahleda Rastislav , Wagner Andrew J. , Burgess Melissa A. , Junker Niels , Chisamore Michael , Peterson Patrick , Szpurka Anna M. , Ceccarelli Matteo , Tap William D.
Published: July 2023

Abstract
Purpose: The study evaluated safety and efficacy of olaratumab+pembrolizumab in patients with unresectable locally advanced/metastatic soft-tissue sarcoma with disease progression on standard treatment. Patients and Methods: This was open-label, multicenter, nonrandomized, phase 1a/1b dose-escalation study followed by cohort expansion (olaratumab+pembrolizumab intravenous infusion). Primary objectives were safety and tolerability. Results: Majority of patients enrolled (41) were female (phase 1a: 9 of 13, phase 1b/dose-expansion cohort [DEC]: 17 of 28), aged <65 years. In phases 1a and 1b, 13 and 26 patients received prior systemic therapy, respectively. Patients received olaratumab 15mg/kg (phase 1a; cohort 1) or 20mg/kg (phase 1a; cohort 2 and phase 1b) and pembrolizumab 200mg (phase 1a/1b). The median (Q1–Q3) duration of therapy (olaratumab) was 6.0 (3.0-11.9) (cohort 1), 14.4 (12.4-20.9) (cohort 2), and 14.0 (6.0-21.8) weeks (DEC). No dose-limiting toxicities and few Grade ≥3 treatment-emergent adverse events (TEAEs, 15 mg/kg: 2 [increased lipase], 20mg/kg: 1 [increased lipase], 1 [colitis], 2 [diarrhea], 3 [anemia]) were reported. Two TEAEs (increased lipase) were related to study discontinuations. Twenty-one patients reported mild (Grade ≤2) TEAE. Phase 1a, disease control rate (DCR):14.3% (1/7, cohort 1); 66.7% (4/6, cohort 2); no responses were reported. Phase 1b, DCR: 53.6% (15/28); objective response rate: 21.4% (6/28) (RECIST and irRECIST criteria). No response in patients with programmed death ligand-1 positive tumors. Conclusions: Antitumor activity was observed in some patients in DEC, combination was well tolerated with manageable safety profile. Further studies are warranted to evaluate efficacy/mechanistic impact of platelet-derived growth factor receptors inhibitor with immune checkpoint modulator coadministration.
Data from Progressive spreading of DNA methylation in the GSTP1 promoter CpG island across transitions from precursors to invasive prostate cancer
Authors: Gupta Harshath , Inoue Hitoshi , Nakai Yasutomo , Nakayama Masashi , Jones Tracy , Hicks Jessica L. , Kumar Balasubramanian , Gurel Meltem , Nelson William G. , De Marzo Angelo M. , Yegnasubramanian Srinivasan
Published: July 2023

Abstract
Glutathione S-transferase pi 1 (GSTP1) is lowly expressed in normal prostate luminal cells and becomes induced in most proliferative inflammatory atrophy lesions (PIA). GSTP1 becomes silenced in prostatic intraepithelial neoplasia (PIN) and prostate adenocarcinoma (CaP) via cytosine-phospho-guanine (CpG) island promoter hypermethylation. However, GSTP1 methylation patterns in PIA and PIN, and their relationship to patterns in CaP are poorly understood. We used bisulfite genomic sequencing to examine patterns of GSTP1 promoter CpG island methylation in laser capture microdissected benign, PIA, PIN, and CaP regions from 32 subjects that underwent radical prostatectomy. We analyzed 908 sequence clones across 24 normal epithelium, 37 PIA, 18 PIN, and 23 CaP regions, allowing assessment of 34,863 CpG sites with allelic phasing. Normal and PIA lesions were mostly unmethylated with 0.52 and 1.3% of total CpG sites methylated, respectively. PIN and CaP lesions had greater methylation with 24% and 51% of total CpG sites methylated, respectively. The degree of GSTP1 methylation showed progression from PIA << PIN < CaP. PIN lesions showed more partial methylation compared to CaP lesions. Partially methylated lesions were enriched for methylation changes at AP1 and SP1 transcription factor binding sites. These results demonstrate that methylation density in the GSTP1 CpG island in PIN was intermediate relative to that in normal prostate epithelium/PIA and CaP lesions. These results are consistent with gradual spreading of DNA methylation centered at the SP1/AP1 transcription factor binding sites in precursor lesions, with subsequent spreading of methylation across the entire CpG island in transition to CaP.
Data from RLY-4008, the first highly selective FGFR2 inhibitor with activity across FGFR2 alterations and resistance mutations
Authors: Subbiah Vivek , Sahai Vaibhav , Maglic Dejan , Bruderek Kamil , Toure B. Barry. , Zhao Songping , Valverde Roberto , O'Hearn Patrick J. , Moustakas Demetri T. , Schonherr Heike , Gerami-Moayed Nastaran , Taylor Alexander M. , Hudson Brandi M. , Houde Damian J. , Pal Debjani , Foster Lindsey , Gunaydin Hakan , Ayaz Pelin , Sharon Dina A. , Goyal Lipika , Schram Alison M. , Kamath Suneel , Sherwin Cori Ann , Schmidt-Kittler Oleg , Jen Kai Yu , Ricard Fabien , Wolf Beni B. , Shaw David E. , Bergstrom Donald A. , Watters James , Casaletto Jessica B.
Published: July 2023

Abstract
Oncogenic activation of fibroblast growth factor receptor 2 (FGFR2) drives multiple cancers and represents a broad therapeutic opportunity, yet selective targeting of FGFR2 has not been achieved. While the clinical efficacy of pan-FGFR inhibitors (pan-FGFRi) validates FGFR2 driver status in FGFR2 fusion-positive intrahepatic cholangiocarcinoma, their benefit is limited by incomplete target coverage due to FGFR1- and FGFR4-mediated toxicities (hyperphosphatemia and diarrhea) and the emergence of FGFR2 resistance mutations. RLY 4008 is a highly selective, irreversible FGFR2 inhibitor designed to overcome these limitations. In vitro, RLY-4008 demonstrates >250- and >5000-fold selectivity over FGFR1 and FGFR4, respectively, and targets primary alterations and resistance mutations. In vivo, RLY-4008 induces regression in multiple xenograft models – including models with FGFR2 resistance mutations that drive clinical progression on current pan-FGFRi – while sparing FGFR1 and FGFR4. In early clinical testing, RLY-4008 induced responses without clinically significant off-isoform FGFR toxicities, confirming the broad therapeutic potential of selective FGFR2 targeting.
Morphological surveys of the sagittal otolith of marine gobiid fish (Teleostei: Gobioidei)
Authors: Iranmanesh Mahdi , Askari Hesni Majid , Lashkari Mohammadreza
Published: July 2023

The challenges fish face in intraspecific communication has resulted in adaptation in different parts of their sensory system, including the auditory system. Evidence suggests that the sensory capability is specific for each species. The otolith is a part of the auditory system of teleost fish, and plays an important role in maintaining balance and in hearing. According to previous research, the morphological variation of otoliths is a result of a synergy between genetic and environmental factors such as depth, diet, swimming, and substrates. In this research we focused to explain (1) morphological variations of the otolith of gobiid fish as an animal inhabiting both aquatic and terrestrial environments; (2) the use of this morphological variations for distinguishing gobiid fish; and (3) how communication systems adapt to the local environment. The sagittal otoliths of six species of gobiid fish were extracted and described. In the next step, we studied the sagittal otoliths using the geometric morphometrics method and shape indices. The most common characteristics of the sagitta in the studied gobies fish were the median and mesial sulcus and the absence of a clear rostrum and antirostrum. A comparison between the examined methods showed that the geometric morphometric approach was able to detect the subtle differences between gobies species. Moreover, it seems that lifestyle and vision could affect the efficiency of the auditory system, in particular the otolith shape. For example, it seems the specific otolith shape of Periophthalmus waltoni (a species that unlike other fish spends most of its time out of the water) perhaps is a result of adaptation to land and water.
Description of a new species of the genus Amphioctopus (Mollusca: Octopodidae) from the Hawai'ian Islands
Authors:   Department of Integrative Biology, 3060 Valley Life Sciences, BLDG #3140, University of California, Berkeley, CA 94720- 3140 USA., HUFFARD CHRISTINE L. , HOCHBERG F. G. ,   Department of Invertebrate Zoology, Santa Barbara Museum of Natural History, 2559 Puesta del Sol Rd, Santa Barbara, CA 93105, USA.
Published: July 2023

Amphioctopus arenicola is a new species of octopus that lives in shallow coastal waters off the Hawai’ian Islands. The species is medium sized (ML to 100 mm); sucker counts range from 160–210 on normal arms and approximately 100 on hectocotylized arms of males; gill counts range from 9–11 lamellae per demibranch; copulatory organs are small, 2.5–3.5% of hectocotylized arm lengths; eggs are small (2–3 mm); and hatchlings planktonic. Amphioctopus arenicola shares a number of characteristics with several non-ocellate tropical Pacific members of the genus, namely, A. aegina (Gray, 1849), A. kagoshimensis (Ortman, 1888), and A. marginatus (Taki, 1964). It can be distinguished from these species based on body size, sucker counts, configuration of the male terminal organ, spermatophore size and number, and geographic distribution. Amphioctopus arenicola typically lives on sandy substrates in depths ranging from 1–80 m.
On the identities of the sesarmid crabs, Sesarma villosum A. Milne-Edwards, 1869, and Clistocoeloma suvaense Edmondson, 1951, with the description of a new species from the West Pacific (Decapoda, Brachyura, Thoracotremata)
Authors: LEE BEE YAN , NG NGAN KEE , NG PETER K. L.
Published: July 2023

The identities of two sesarmid crabs, Clistocoeloma villosum (A. Milne-Edwards, 1869), and C. suvaense Edmondson, 1951, both currently in Clistocoeloma A. Milne-Edwards, 1873, are poorly known and the types have not been studied in detail since their description. Clistocoeloma villosum, supposedly widespread, has been recorded from Madagascar and East Africa to Singapore, Japan, Korea, and Samoa. Clistocoeloma suvaense is only known for certain from one male collected from its type locality, Fiji. The taxonomy of C. villosum, and C. suvaense is clarified on the basis of the type specimens from Samoa and Fiji, respectively. A new species, which has long been confused with C. villosum, is described from Southeast Asia and Western Pacific.
Combination Analyses Next Generation Sequencing of Lung Adenocarcinoma ctDNA and CTCs Based on Multi-Site Immunomagnetic Beads
Authors: Li Jia , Ding Jun , Xu Fang
Published: July 2023

Background: Lung cancer is the most important disease that endangers human health worldwide. High research value exists for liquid biopsy in predicting treatment response, early diagnosis of disease recurrence, and real-time dynamic detection of tumor evolution, and each one
have their own unique advantages and drawbacks. Methods: We used a novel combined Epcam immunomagnetic liposome bead (Ep-IML) and Vimentin immunomagnetic liposome bead (Vi-IML) tumor cell enrichment strategy to capture CTCs from 30 lung adenocarcinoma (LAC) patients and then performed
high-throughput sequencing with Circulating tumor cell DNA (ctcDNA) and ctDNA to understand the genetic variants of the patients. Results: The modified CTC enrichment efficiency was significantly improved and the mean value of CTCs enriched when Ep-IML combined with Vi-IML is 11.78/7.5
mL. The results of genomic analysis of CTC in lung adenocarcinoma showed that the five most frequently mutated genes were EGFR, TP53, KRAS, ALK, BRAF. And results of ctDNA gene analysis in lung adenocarcinoma patients showed that the five most frequently mutated genes were EGFR, AKT1, TP53,
DDR2, and FGFR3. NGS analysis demonstrated that variations in the genetic profile revealed by the liquid biopsy might be increased by combining tests with CTC and ctDNA. Conclusion: We have developed a customized CTC enrichment identification system. CTCs could be an ideal complement
to ctDNA and have important clinical applications in guiding clinical dosing and individualized therapy, combined CTC and ctDNA assays could detect as many drug-available targets as possible for a patient in a single trial.
Machine Learning Analysis of Microtensile Bond Strength of Dental Adhesives
Authors: Wang R. , Hass V. , Wang Y.
Published: July 2023

Dental adhesives provide retention to composite fillings in dental restorations. Microtensile bond strength (µTBS) test is the most used laboratory test to evaluate bonding performance of dental adhesives. The traditional approach for developing dental adhesives involves repetitive laboratory measurements, which consumes enormous time and resources. Machine learning (ML) is a promising tool for accelerating this process. This study aimed to develop ML models to predict the µTBS of dental adhesives using their chemical features and to identify important contributing factors for µTBS. Specifically, the chemical composition and µTBS information of 81 dental adhesives were collected from the manufacturers and the literature. The average µTBS value of each adhesive was labeled as either 0 (if <36 MPa) or 1 (if ≥36 MPa) to denote the low and high µTBS classes. The initial 9-feature data set comprised pH, HEMA, BisGMA, UDMA, MDP, PENTA, filler, fluoride, and organic solvent (OS) as input features. Nine ML algorithms, including logistic regression, k-nearest neighbor, support vector machine, decision trees and tree-based ensembles, and multilayer perceptron, were implemented for model development. Feature importance analysis identified MDP, pH, OS, and HEMA as the top 4 contributing features, which were used to construct a 4-feature data set. Grid search with stratified 10-fold cross-validation (CV) was employed for hyperparameter tunning and model performance evaluation using 2 metrics, the area under the receiver operating characteristic curve (AUC) and accuracy. The 4-feature data set generated slightly better performance than the 9-feature data set, with the highest AUC score of 0.90 and accuracy of 0.81 based on stratified CV. In conclusion, ML is an effective tool for predicting dental adhesives with low and high µTBS values and for identifying important chemical features contributing to the µTBS. The ML-based data-driven approach has great potential to accelerate the discovery of new dental adhesives and other dental materials.
Young Adult Medical Cannabis Patients in Florida: Gender Differences in Characteristics of use, Experiences at Dispensaries, and Association With Health-Related Problems
Authors: Lorenzo Elise , McClean Carina , Ford Jason A.
Published: July 2023

In recent years there has been an increase in cannabis use among adults in the U.S., which corresponds with changes in state-level laws making cannabis available for medical/recreational use. While cannabis became available for medical use in Florida in 2014, it was not until a ban on smoking cannabis was lifted in 2019 that the number of patients began to increase. The data for the current study are the Florida Young Adult Cannabis Study and focus on 415 medical cannabis patients (MCP). We identified several significant differences between male and female MCP. Female MCP initiated regular cannabis use at a younger age and reported more frequent cannabis use. Female MCP were more likely to endorse self-treatment motives while male MCP were more likely to endorse recreational motives. As Florida is one of the largest and most diverse states in the U.S., research on MCP is needed to inform policy.
Cannabis Consumption Among Adults Aged 55–65 in Canada, 2018–2021
Authors: Wadsworth Elle , Cristiano Nick , Gabrys Robert , Renard Justine , Hammond David
Published: July 2023

Cannabis consumption among aging adults in Canada is increasing. The aims of the study were to examine cannabis consumption patterns before and after non-medical cannabis legalization and assess whether these patterns differ between men and women. Data were analyzed from Canadian respondents in a repeat cross-sectional survey conducted in 2018–2021. Analyses were conducted among adults aged 55–65 ( n = 18,177) who had consumed cannabis in the past 12-month ( n = 4119). Past 12-month cannabis consumption significantly increased among 55–65-year-olds from 2018 (19.3%) to the first-year post-legalization in 2019 (24.5%; p < .001), but remained stable thereafter (24.3%, and 25.6% in 2020 and 2021). More men reported past 12-month consumption than women (28.4% vs. 21.4%; p < .001). A substantial number of cannabis consumers consumed to manage a physical or mental health condition. Targeted messaging might be beneficial for this age group, including possible interactions with other medications. This research may be helpful for informing age-adapted cannabis education.
The Moral Perspectives of the Anaphora of Addai and Mari in the Syro-Malabar Liturgy
Authors: Kanniyakonil Scaria
Published: July 2023

Liturgical prayers comprise a myriad of moral concepts. This is obvious in the anaphora of Addai and Mari, one of the earliest liturgies in the East Syrian tradition. Prayers of this anaphora are a source of Christian moral tradition. This article argues that there are fundamental moral concepts, moral values, and special moral cases in the anaphora of Addai and Mari. The prominent moral themes of this anaphora include conscience, justice, love, communion, holiness, gratitude, mercy, peace, and eco-theology. Moreover, participation in the Holy Qurbana incites moral imagination and subsequently helps one to arrive at good moral decisions. The result is the moral response (counter-gift) of the participation in the Holy Qurbana. The article also explores the similarities between the moral concepts of Acts of Thomas, Ephrem's hymns, Aphrahat's Demonstrations and the anaphora of Addai and Mari. This article reasons that a deep relationship exists between liturgy and morality, and one can obtain mature moral perspectives from the anaphora of Addai and Mari in the present context.
MiR-128-3p promotes the progression of deep venous thrombosis through binding SIRT1
Authors: Han Jinan , Hao Wanjiang , Ma Yanping , Hou Yanqiu
Published: July 2023

Objectives

This research aimed to study the effect of microRNA-128-3p (miR-128-3p) on deep venous thrombosis (DVT).

Method

The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, Transwell chamber method, and flow cytometry technique were used in the cell experiments. Potential interconnection between miR-128-3p and silent information regulator sirtuin 1 (SIRT1) was revealed by luciferase activity. The concentration of miR-128-3p and mRNA SIRT1 was assessed by quantitative reverse transcription polymerase chain reaction (qRT-PCR). The receiver operating characteristic (ROC) curve was used to test the predictive effect of miR-128-3p in DVT.

Results

Decreased miR-128-3p expression was beneficial to cell proliferation and migration and inhibited inflammation, apoptosis, and adhesion of human umbilical vein endothelial cells (HUVECs). The impacts of miR-128-3p on HUVECs were achieved by targeting SIRT1. MiR-128-3p was upregulated in patients with DVT, and it was of great significance in differentiating patients with DVT.

Conclusion

Overexpression of miR-128-3p might become a biomarker for patients with DVT.