bluuebunny/crossref_metadata_2025 huggingface dataset:
Associations between Pharmacotherapy for Cardiovascular Diseases and Periodontitis
Authors: Pająk-Łysek Ewa , Polak Maciej , Kopeć Grzegorz , Podolec Mateusz , Desvarieux Moïse , Pająk Andrzej , Zarzecka Joanna
Published: January 2021

The goal of the study was to assess the relationship between cardioprotective medications, i.e., beta-blockers, angiotensin-converting enzyme inhibitors (ACEIs), calcium channel blockers (CCBs), angiotensin II receptor blockers (ARBs), statins, acetylsalicylic acid (ASA), and periodontitis (PD). Background: Xerostomia increases the risk of PD and is a side effect of some pharmacotherapies. Information about the effect of cardioprotective treatment of periodontal status is scarce. Methods: We studied 562 dentate residents of Krakow at the age of 50 to 70 years. Information about treatment was collected using a standardized questionnaire. The pocket depth and clinical attachment level (CAL) were used to ascertain PD. Multivariate logistic regression was applied to assess the relation between cardioprotective medications and PD. Results: PD was found in 74% of participants. The range of cardioprotective drug use among participants was 7% (ARBs) to 32% (beta-blockers). After adjusting for age, sex, number of teeth, smoking, and education, ASA’s use was related to a lower prevalence of PD in all dentate participants (odds ratio (OR) = 0.63, 95% confidence interval (CI): 0.40–0.99). The use of ARBs and statins was found to be associated with a higher prevalence of PD in persons having ≥6 teeth (odds ratio (OR) = 3.57, 95% CI: 1.06–11.99 and OR = 1.81, 95% CI: 1.03–3.16, respectively). Further adjustment for CVD risk factors, history of coronary heart disease, and other chronic diseases did not attenuate the results. There was no significant relation between PD and the use of other cardioprotective drugs.
Impact of Real and Simulated Flights on Psychophysiological Response of Military Pilots
Authors: Fuentes-García Juan Pedro , Clemente-Suárez Vicente J. , Marazuela-Martínez Miguel Ángel , Tornero-Aguilera José F. , Villafaina Santos
Published: January 2021

Objective: The present research aimed to analyse the autonomic, anxiety, perceived exertion, and self-confidence response during real and simulated flights. Methods: This cross-sectional study participated 12 experienced male pilots (age = 33.08 (5.21)) from the Spanish Air Force. Participants had to complete a real and a simulated flight mission randomly. The heart rate variability (HRV), anxiety, self-confidence, and rating of perceived exertion were collected before and after both manoeuvres, and HRV was also collected during both simulated and real flights. Results: When studying the acute effects of real and simulated flights, the mean heart rate, the R-to-R interval, the cognitive anxiety and the perceived exertion were significantly impacted only by real flights. Furthermore, significant differences in the mean heart rate and RR interval were found when compared to the acute effects of real and simulated flights (with higher acute effects observed in real flights). Additionally, when compared the HRV values during simulated and real flights, significant differences were observed in the RR and heart rate mean (with lower RR interval and higher heart rate mean observed during real flights). Conclusion: Real flights significantly reduced the RR interval and cognitive anxiety while increased the heart rate mean and the rating of perceived exertion, whereas simulated flights did not induce any significant change in the autonomic modulation.
Is Visual Pedagogy Effective in Improving Cooperation towards Oral Hygiene and Dental Care in Children with Autism Spectrum Disorder? A Systematic Review and Meta-Analysis
Authors: Balian Araxi , Cirio Silvia , Salerno Claudia , Wolf Thomas Gerhard , Campus Guglielmo , Cagetti Maria Grazia
Published: January 2021

Visual pedagogy has emerged as a new approach in improving dental care in children with autism spectrum disorders (ASDs). This paper aimed to evaluate and assess the scientific evidence on the use of visual pedagogy in improving oral hygiene skills and cooperation during dental care in children with ASDs. The review protocol was registered on the PROSPERO Register (CRD42020183030). Prospective clinical studies, randomized trials, interruptive case series, before and after comparison studies, and cross-sectional studies following the PRISMA guideline were searched in PubMed, Embase, Scopus, and Google Scholar using ad hoc prepared search strings. The search identified 379 papers, of which 342 were excluded after title and abstract evaluation, and 37 full-text papers were analyzed. An additional four papers were added after consulting reference lists. Eighteen papers were disregarded; 23 were finally included, and their potential bias was assessed using ROB-2 and ROBINS-I tools. The wide heterogenicity of the studies included does not allow for conclusive evidence on the effectiveness of visual pedagogy in oral hygiene skills and dental care. Nevertheless, a significant and unilateral tendency of the overall outcomes was found, suggesting that visual pedagogy supports ASD children in improving both oral hygiene skills and cooperation during dental care.
Preliminary Results of the Planet Nutrition Program on Obesity Parameters in Mexican Schoolchildren: Pilot Single-School Randomized Controlled Trial
Authors: Ramírez-Rivera Diana L. , Martínez-Contreras Teresita , Villegas-Valle Rosa C. , Henry-Mejia Gricelda , Quizán-Plata Trinidad , Haby Michelle M. , Díaz-Zavala Rolando G.
Published: January 2021

School-based obesity prevention programs are key to promoting healthy habits. The aim of this study was to evaluate the effect of the Planet Nutrition program on BMI z-score and other parameters compared to a control group of Mexican schoolchildren after 9 weeks of intervention. The effect of the summer holidays on the BMI z-score was also evaluated at 23 weeks. A pilot randomized controlled trial design was used and 41 schoolchildren were randomized (21 intervention group and 20 control). The program included 18 nutrition education sessions, 20 physical activity classes and six brochures for parents. At 9 weeks, no significant differences were found between the intervention and control groups in the change in BMI z-score (−0.11, 95% CI −0.23, 0.01). Significant differences were observed in some secondary outcomes: body fat percentage (−1.72, 95% CI −3.42, −0.02), waist circumference (−3.45, 95% CI −5.55, −1.36), physical activity (0.44, 95% CI 0.01, 0.88) and nutrition knowledge (1.15, 95% CI 0.27, 2.03). Summer holidays negatively affected the BMI z-score in both groups, reducing the difference observed between groups at 9 weeks (−0.07, 95% CI −0.22, 0.07). The Planet Nutrition program showed favorable effects in some obesity and lifestyle parameters in the short term.
Reply to Detection of Leptospira interrogans DNA in Urine of a Captive Ocelot (Leopardus pardalis)
Authors: Murillo Andrea , Cuenca Rafaela , Serrano Emmanuel , Marga Goris , Ahmed Ahmed , Cervantes Salvador , Caparrós Cristina , Vieitez Verónica , Ladina Andrea , Pastor Josep
Published: January 2021

In reply to a comment, we want to mention that we consider this report significant in animal leptospirosis [...]
Physical Environment vs. Social Environment: What Factors of Age-Friendliness Predict Subjective Well-Being in Men and Women?
Authors: del Barrio Elena , Pinzón Sandra , Marsillas Sara , Garrido Francisco
Published: January 2021

“Age-Friendly Cities and Communities” is an initiative launched by the WHO in 2007 that has spread to more than 1000 cities and communities around the world. This initiative is based on an integrated physical and social environment for older people, and a model of participatory, collaborative governance. An enabling social environment setting is just as important as material conditions in determining well-being in later life. The objective of this study is to analyze the interaction between age-friendliness (physical and social) and subjective well-being in women and men aged 55 and over in the Basque Country. The methodology was based on a survey of a representative sample (n = 2469 individuals). In order to know the predictive power of age-friendliness over subjective well-being, linear regression models separated by gender were constructed. The predictive models of age-friendliness are composed by different variables for men and women. In both cases, the physical environment variables do not remain in the final model. Among the predictors of well-being in men, the coexistence stands out as a safety and support network. In women, the neighborhood has proved to be a very important resource. The conclusions of this study contribute to literature and interventions promoting more effective strategies that enhance older people well-being, considering the gender perspective.
Combined Effects of Elevated Temperature and Crude Oil Pollution on Oxidative Stress and Apoptosis in Sea Cucumber (Apostichopus japonicus, Selenka)
Authors: Li Xishan , Wang Chengyan , Li Nan , Gao Yali , Ju Zhonglei , Liao Guoxiang , Xiong Deqi
Published: January 2021

Currently, global climate change and oil pollution are two main environmental concerns for sea cucumber (Apostichopus japonicus) aquaculture. However, no study has been conducted on the combined effects of elevated temperature and oil pollution on sea cucumber. Therefore, in the present study, we treated sea cucumber with elevated temperature (26 °C) alone, water-accommodated fractions (WAF) of Oman crude oil at an optimal temperature of 16 °C, and Oman crude oil WAF at an elevated temperature of 26 °C for 24 h. Results showed that reactive oxygen species (ROS) level and total antioxidant capacity in WAF at 26 °C treatment were higher than that in WAF at 16 °C treatment, as evidenced by 6.03- and 1.31-fold-higher values, respectively. Oxidative damage assessments manifested that WAF at 26 °C treatment caused much severer oxidative damage of the biomacromolecules (including DNA, proteins, and lipids) than 26 °C or WAF at 16 °C treatments did. Moreover, compared to 26 °C or WAF at 16 °C treatments, WAF at 26 °C treatment induced a significant increase in cellular apoptosis by detecting the caspase-3 activity. Our results revealed that co-exposure to elevated temperature and crude oil could simulate higher ROS levels and subsequently cause much severer oxidative damage and cellular apoptosis than crude oil alone on sea cucumber.
Process Simulation and Environmental Aspects of Dimethyl Ether Production from Digestate-Derived Syngas
Authors: Giuliano Aristide , Catizzone Enrico , Freda Cesare
Published: January 2021

The production of dimethyl ether from renewables or waste is a promising strategy to push towards a sustainable energy transition of alternative eco-friendly diesel fuel. In this work, we simulate the synthesis of dimethyl ether from a syngas (a mixture of CO, CO2 and H2) produced from gasification of digestate. In particular, a thermodynamic analysis was performed to individuate the best process conditions and syngas conditioning processes to maximize yield to dimethyl etehr (DME). Process simulation was carried out by ChemCAD software, and it was particularly focused on the effect of process conditions of both water gas shift and CO2 absorption by Selexol® on the syngas composition, with a direct influence on DME productivity. The final best flowsheet and the best process conditions were evaluated in terms of CO2 equivalent emissions. Results show direct DME synthesis global yield was higher without the WGS section and with a carbon capture equal to 85%. The final environmental impact was found equal to −113 kgCO2/GJ, demonstrating that DME synthesis from digestate may be considered as a suitable strategy for carbon dioxide recycling.
Bayesian Meta-Analysis for Binary Data and Prior Distribution on Models
Authors: Negrín-Hernández Miguel-Angel , Martel-Escobar María , Vázquez-Polo Francisco-José
Published: January 2021

In meta-analysis, the structure of the between-sample heterogeneity plays a crucial role in estimating the meta-parameter. A Bayesian meta-analysis for binary data has recently been proposed that measures this heterogeneity by clustering the samples and then determining the posterior probability of the cluster models through model selection. The meta-parameter is then estimated using Bayesian model averaging techniques. Although an objective Bayesian meta-analysis is proposed for each type of heterogeneity, we concentrate the attention of this paper on priors over the models. We consider four alternative priors which are motivated by reasonable but different assumptions. A frequentist validation with simulated data has been carried out to analyze the properties of each prior distribution for a set of different number of studies and sample sizes. The results show the importance of choosing an adequate model prior as the posterior probabilities for the models are very sensitive to it. The hierarchical Poisson prior and the hierarchical uniform prior show a good performance when the real model is the homogeneity, or when the sample sizes are high enough. However, the uniform prior can detect the true model when it is an intermediate model (neither homogeneity nor heterogeneity) even for small sample sizes and few studies. An illustrative example with real data is also given, showing the sensitivity of the estimation of the meta-parameter to the model prior.
Enhanced Phytoremediation of Bisphenol A in Polluted Lake Water by Seedlings of Ceratophyllum demersum and Myriophyllum spicatum from In Vitro Culture
Authors: Zhao Chong , Zhang Guosen , Jiang Jinhui
Published: January 2021

Bisphenol A (BPA) is a typical endocrine disruptor that causes problems in waters all around the world. In this study, the effects of submerged macrophytes (Ceratophyllum demersum and Myriophyllum spicatum) cultured in vitro on the removal of BPA at two initial concentrations (0.5 mg L−1 vs. 5.0 mg L−1) from Donghu lake water were investigated, using different biomass densities (2 g L−1 vs. 10 g L−1) under different nutrient conditions (1.85 mg L−1 and 0.039 mg L−1 vs. 8.04 mg L−1 and 0.175 mg L−1 of the total nitrogen and phosphorus concentration, respectively), together with the effect of indigenous microorganisms in the water. The results showed that indigenous microorganisms had limited capacity for BPA removal, especially at higher BPA initial concentration when its removal rate amounted to about 12% in 12 days. Addition with plant seedlings (5 cm in length) greatly enhanced the BPA removal, which reached 100% and over 50% at low and high BPA initial concentration in 3 days, respectively. Higher biomass density greatly favored the process, resulting in 100% of BPA removal at high BPA initial concentration in 3 days. However, increases in nutrient availability had little effect on the BPA removal by plants. BPA at 10.0 mg L−1 significantly inhibited the growth of M. spicatum. Therefore, C. demersum may be a candidate for phytoremediation due to greater efficiency for BPA removal and tolerance to BPA pollution. Overall, seedlings of submerged macrophytes from in vitro culture showed great potential for use in phytoremediation of BPA in natural waters, especially C. demersum.
Prevalence of Asymptomatic Malaria Infections in Seemingly Healthy Children, the Rural Dzanga Sangha Region, Central African Republic
Authors: Korzeniewski Krzysztof , Bylicka-Szczepanowska Emilia , Lass Anna
Published: January 2021

According to the World Health Organization 94% of global malaria cases and 94% of global malaria deaths have been reported from Africa. Unfortunately, it is difficult to determine the exact prevalence of disease in some African countries due to a large number of asymptomatic cases. The aim of this study was to assess the prevalence of malaria infections in seemingly healthy children living in the Central African Republic (CAR). CareStartTM Malaria HRP2 rapid diagnostic test (RDT) targeting Plasmodium falciparum was used to test a group of 500 asymptomatic children aged 1-15 years old (330 settled Bantu and 170 semi-nomadic BaAka Pygmies) inhabiting the villages in the Dzanga Sangha region (south-west CAR) in March 2020. In total, 32.4% of asymptomatic Bantu and 40.6% of asymptomatic Pygmy children had a positive result of malaria RDT. Our findings allowed us to demonstrate the high prevalence of asymptomatic malaria infections in south-west CAR. RDTs seem to be a useful tool for the detection of Plasmodium falciparum in areas with limited possibilities of using other diagnostic methods, such as light microscopy and molecular biology.
The Effect of Two Bed Bath Practices in Cost and Vital Signs of Critically Ill Patients
Authors: Tai Chia-Hui , Hsieh Tsung-Cheng , Lee Ru-Ping
Published: January 2021

By promoting personal hygiene and improving comfort, bed baths can decrease the risk of infection and help maintain skin integrity in critically ill patients. Current bed-bathing practices commonly involve the use of either soap and water (SAW) or disposable wipes (DWs). Previous research has shown both bed-bathing methods are equally effective in removing dirt, oil, and microorganisms. This experimental study compared the cost, staff satisfaction, and effects of two bed-bathing practices on critically ill patients’ vital signs. We randomly assigned 138 participants into 2 groups: an experimental group that received bed baths using DWs and a control group that received bed baths using SAW. We compared the bath duration, cost, vital sign trends, and nursing staff satisfaction between the two groups. We used the chi-square test and t-test for the statistical analysis, and we expressed the quantitative data as mean and standard deviation. Our results showed the bed baths using DWs had a shorter duration and lower cost than those using SAW. There were no significant differences in the vital sign trends between the two groups. The nursing staff preferred to use DWs over SAW. This study can help clinical nursing staff decide which method to use when assisting patients with bed baths.
Big Five Personality Traits, Coping Strategies and Compulsive Buying in Spanish University Students
Authors: Otero-López José Manuel , Santiago María José , Castro María Cristina
Published: January 2021

Personality traits and coping strategies have historically been two key elements in the field of health psychology. It is, therefore, striking that there is no study in the field of compulsive buying that integrates the most generic, decontextualized and stable aspects (traits) with those having a more marked processual and dynamic nature, which are closer to goal-based views of human nature (coping strategies). Another weakness of the compulsive buying field is that, despite the confirmed growing increase in compulsive buying in the younger age groups, most studies have been conducted with adult samples. Hence, this study seeks to clarify the role of the Big Five domains and different coping strategies in university students’ compulsive buying. The sample consisted of 1093 participants who were classified as either compulsive buyers or non-compulsive buyers. Both groups were compared regarding sociodemographic variables (gender, age), the Big Five personality traits, and coping strategies through chi-square tests or Student’s t-tests. Besides, a multivariate logistic regression analysis was conducted to determine which of these determinants might play a part in the construction of a risk profile for compulsive buying. The results showed that other than gender (specifically being female), Neuroticism and the use of such coping strategies as problem avoidance and wishful thinking are risk factors that increase the propensity for compulsive buying. The use of active coping strategies such as problem solving, cognitive restructuring and social support, as well as the Conscientiousness dimension are protection factors that decrease the likelihood of becoming a compulsive buyer. Finally, and on the basis of the findings obtained, possible guidelines are given, which, hopefully, may effectively contribute to the prevention of and/or intervention in compulsive buying among young adults.
Norepinephrine Infusion in the Emergency Department in Septic Shock Patients: A Retrospective 2-Years Safety Report and Outcome Analysis
Authors: Messina Antonio , Milani Angelo , Morenghi Emanuela , Costantini Elena , Brusa Stefania , Negri Katerina , Alberio Daniele , Leoncini Ornella , Paiardi Silvia , Voza Antonio , Cecconi Maurizio
Published: January 2021

Hemodynamic optimization during sepsis and septic shock is based on a prompt and large fluid resuscitation strategy associated with early administration of norepinephrine. In our hospital, norepinephrine is administered in the emergency department (ED), within a protocol-guided management context, to reduce norepinephrine infusion timing due to central line insertion. This choice, however, can be associated with side effects. Objectives: We conducted a retrospective analysis regarding the safety of norepinephrine in the ED. We also appraised the association between in-hospital mortality and predefined ED variables and patients’ admission severity scores. Design, settings, and participants: This was a retrospective analysis of electronic sheets of the ED of a tertiary hospital in the North of Italy. Outcomes measure and analysis: Electronic documentation was assessed to identify local and systemic side effects. We considered two subgroups of patients according to the in-hospital clinical paths: (1) those admitted in the intensive care unit (ICU); and (2) those who received a ceiling of care decision. We collected and considered variables related to septic shock treatment in the ED and analyzed their association with in-hospital mortality. Main Results: We considered a two-year period, including 108,033 ED accesses, and ultimately analyzed data from 127 patients. Side effects related to the use of this drug were reported in five (3.9%) patients. Thirty patients (23.6%) were transferred to the ICU from the ED, of whom six (20.0%) died. Twenty-eight patients (22.0%) received a ceiling of care indication, of whom 21 (75.0%) died. Of the 69 (54.3%) finally discharged to either medical or surgical wards, 21 (30.4%) died. ICU admission was the only variable significantly associated to in-hospital mortality in the multivariable analysis [OR (95% CI) = 4.48 (1.52–13.22); p-value = 0.007]. Conclusions: Norepinephrine peripheral infusion in the ED was associated with a low incidence of adverse events requiring discontinuation (3.9%). It could be considered safe within <12 h when a specific line management protocol and pump infusion protocol are adopted. None of the variables related to septic shock management affected in-hospital mortality, except for the patient’s ICU admission.
Development of a Community-Based Integrated Service Model of Health and Social Care for Older Adults Living Alone
Authors: Yi Yu Mi , Park Yeon-Hwan , Cho BeLong , Lim Kyung-Choon , Jang Soong-Nang , Chang Sun Ju , Ko Hana , Noh Eun-Young , Ryu So Im
Published: January 2021

The number of elderly people living alone worldwide is increasing, and the responsibility of the state in this context is emerging. This study aimed to develop a community-based integrated service (CBIS) model of health and social care for older adults living alone. The model was designed based on a literature review of previous community care models and per older adults’ health and daily life needs. Thereafter, feedback on the integrated model was taken from older adults living alone by conducting a survey (n = 1023) and focus group interviews, after which the opinions of the Public type Health Management Promotion Council were considered and content validity was confirmed. The model, comprising eight healthcare services and five social care services, was tested on 22 older adults for two weeks to assess its feasibility and preliminary efficiency. Each service included screening, assessment, providing service, evaluation, and quit. Participants rated their overall satisfaction with the services as 9 out of 10. Care navigators reported feeling comforted and discovered their own sense of being while providing the services. We believe that the CBIS model may foster independence among community-dwelling older adults living alone, thereby improving their quality of life through “aging in place”.
Effects of Combined Exercise and Low Carbohydrate Ketogenic Diet Interventions on Waist Circumference and Triglycerides in Overweight and Obese Individuals: A Systematic Review and Meta-Analysis
Authors: Lee Hyun Suk , Lee Junga
Published: January 2021

(1) Background: The purpose of this meta-analysis was to investigate the effects of combined exercise and low carbohydrate ketogenic diet interventions (CELCKD) for overweight and obese individuals. (2) Methods: Relevant studies were searched by using the MEDLINE and EMBASE databases up to October 2020. Study Inclusion and Exclusion Criteria: Inclusion criteria were reporting effects of the CELCKD for overweight and obese individuals from randomized controlled trials. Studies that did not match the inclusion criteria were excluded. The methods for CELCKD and outcomes of selected studies were extracted. The effect sizes for interventions that included cardiorespiratory fitness, body composition, fasting glucose, and lipid profiles were calculated by using the standardized mean difference statistic. (3) Results: A total of seven studies and 278 overweight and obese individuals were included. The average intervention of selected studies consisted of moderate to vigorous intensity, 4 times per week for 9.2 weeks. Participating in CELCKD interventions was decreased triglycerides (d = −0.34, CI; −0.68–−0.01, p = 0.04) and waist circumference (d = −0.74, 95% confidence interval [CI]; −1.28–−1.20, p = 0.01), while cardiovascular fitness, body composition, fasting glucose, total cholesterol, high density lipoprotein (HDL) cholesterol, and low density lipoprotein (LDL) cholesterol were not statistically different after the interventions. No adverse side effects were reported. (4) Conclusions: Participation in interventions by overweight and obese individuals had beneficial effects including decreased waist circumference and triglycerides. Longer term intervention studies with homogenous control groups may be needed.
Deploying Spatial Data for Coastal Community Resilience: A Review from the Managerial Perspective
Authors: Huang Xiameng , Song Yanqing , Hu Xuan
Published: January 2021

The use of spatial data for coastal community resilience applications has diversified as a consequence of the increasing availability of data, and extensive development in data processing. However, the true value of spatial data is not fully exploited as a result of lacking scientific managerial models that incorporate spatial data into decision-making. This article synthesizes the cross-disciplinary literature review on deploying spatial data for coastal community resilience from the managerial perspective. It systematically reviews research addressing the topic of deploying spatial data for coastal resilience operations from the earliest available to 1999. The review uses 142 studies to address three research questions: (1) What kind of data can be obtained for coastal resilience situational awareness? (2) What outcomes have spatial data attributed to coastal resilience applications? and (3) What are the missing pieces (gaps) in connecting the spatial data with coastal resilience applications? In addressing these research questions, the authors review articles based on three dimensions including the availability of spatial data, the availability of applications, and limitations. Based on the findings of the analysis, the authors conclude that the managerial perspective of deploying spatial data in coastal hazards are understudies, and outline problem formulation, mission prioritization, and information salience as an agenda for future research.
Changes in the Impacts of Topographic Factors, Soil Texture, and Cropping Systems on Topsoil Chemical Properties in the Mountainous Areas of the Subtropical Monsoon Region from 2007 to 2017: A Case Study in Hefeng, China
Authors: Li Qing , Gu Fenlan , Zhou Yong , Xu Tao , Wang Li , Zuo Qian , Xiao Liang , Liu Jingyi , Tian Yang
Published: January 2021

Understanding the spatial pattern of soil chemical properties (SCPs) together with topological factors and soil management practices is essential for land management. This study examines the spatial changes in soil chemical properties and their impact on China’s subtropical mountainous areas. In 2007 and 2017, 290 and 200 soil samples, respectively, were collected in Hefeng County, a mountainous county in central China. We used descriptive statistics and geostatistical methods, including ANOVA, semivariance, Moran’s I, and fractal dimensions, to analyze the characteristics and spatial autocorrelation changes in soil organic matter (OM), available phosphorus (AP), available potassium (AK), and pH value from 2007 to 2017. We explored the relationship between each SCP and the relationship between SCPs with topographic parameters, soil texture, and cropping systems. The results show that the mean value of soil OM, AP, AK, and pH in Hefeng increased from 2007 to 2017. The spatial variation and spatial dependency of each SCP in 2007, excluding AP and AK in 2007, were higher than in 2017. The soil in areas with high topographic relief, profile curvature, and planform curvature had less AP, AK, and pH. Soil at higher elevation had lower OM (r = −0.197, p < 0.01; r = −0.334, p < 0.01) and AP (r = −0.043, p < 0.05; r = −0.121, p < 0.05) and higher AK (r = −0.305, p < 0.01; r = 0.408, p < 0.01) in 2007 and 2017. Soil OM and AK in 2007 were significantly (p < 0.05) correlated with soil texture (p < 0.05). In contrast, oil AP and soil pH in 2007 and all SCPs in 2017 were poorly correlated with soil texture. The cropping systems played an important role in affecting all SCPs in 2007 (p < 0.01), while they only significantly affected AK in 2017 (p < 0.05). Our findings demonstrate that both topological factors, that is, the changes in cropping management and the changes in acid rain, impact soil chemical properties. The local government should place more focus on reducing soil acid amounts, soil AP content, and soil erosion by improving water conservancy facilities.
Using Restaurant POI Data to Explore Regional Structure of Food Culture Based on Cuisine Preference
Authors: Jiang Shangjing , Zhang Haiping , Wang Haoran , Zhou Lei , Tang Guoan
Published: January 2021

As a result of the influence of geographical environment and historical heritage, food preference has significant regional differentiation characteristics. However, the spatial structure of food culture represented by the cuisine culture at the regional level has not yet been explored from the perspective of geography. Cultural regionalization is an important way to analyze and understand the spatial structure of food culture. It is of great significance to deeply mine intra-regional homogeneity and scientifically cognize inter-regional cultural characteristics. This study aims to explore such patterns by focusing on the restaurants of the eight most famous cuisines in Mainland China. Initially, the density based geospatial hotspot detector method is proposed to analyze and mapping the spatial quantitative characteristics of the eight major cuisines. A heuristic method for geographical regionalization based on machine learning was used to analyze spatial distribution patterns in accordance with the proportion of these cuisines in each prefecture-level city. Results show that some types of single-category cuisines have a stronger spatial concentration effect in the present, whereas others have a strong diffusion trend. In the comprehensive analysis of multicategory cuisines, the eight major cuisines formed a new structure of geographical regionalization of Chinese cuisine culture. This study is helpful to understand regional structure characteristics of food preference, and the density-based hotspot detector proposed in this paper can also be used in the analysis of other type of point of interest (POI) data.
Transcriptome Analysis Revealed GhWOX4 Intercedes Myriad Regulatory Pathways to Modulate Drought Tolerance and Vascular Growth in Cotton
Authors: Sajjad Muhammad , Wei Xi , Liu Lisen , Li Fuguang , Ge Xiaoyang
Published: January 2021

Cotton is a paramount cash crop around the globe. Among all abiotic stresses, drought is a leading cause of cotton growth and yield loss. However, the molecular link between drought stress and vascular growth and development is relatively uncharted. Here, we validated a crucial role of GhWOX4, a transcription factor, modulating drought stress with that of vasculature growth in cotton. Knock-down of GhWOX4 decreased the stem width and severely compromised vascular growth and drought tolerance. Conversely, ectopic expression of GhWOX4 in Arabidopsis enhanced the tolerance to drought stress. Comparative RNAseq analysis revealed auxin responsive protein (AUX/IAA), abscisic acid (ABA), and ethylene were significantly induced. Additionally, MYC-bHLH, WRKY, MYB, homeodomain, and heat-shock transcription factors (HSF) were differentially expressed in control plants as compared to GhWOX4-silenced plants. The promotor zone of GhWOX4 was found congested with plant growth, light, and stress response related cis-elements. differentially expressed genes (DEGs) related to stress, water deprivation, and desiccation response were repressed in drought treated GhWOX4-virus-induced gene silencing (VIGS) plants as compared to control. Gene ontology (GO) functions related to cell proliferation, light response, fluid transport, and flavonoid biosynthesis were over-induced in TRV: 156-0 h/TRV: 156-1 h (control) in comparison to TRV: VIGS-0 h/TRV: VIGS-1 h (GhWOX4-silenced) plants. This study improves our context for elucidating the pivotal role of GhWOX4 transcription factors (TF), which mediates drought tolerance, plays a decisive role in plant growth and development, and is likely involved in different regulatory pathways in cotton.
Glucagon-Like Peptide 1 and Taste Perception: From Molecular Mechanisms to Potential Clinical Implications
Authors: Jensterle Mojca , Rizzo Manfredi , Janez Andrej
Published: January 2021

Preclinical studies provided some important insights into the action of glucagon-like peptide 1 (GLP-1) in taste perception. This review examines the literature to uncover some molecular mechanisms and connections between GLP-1 and the gustatory coding. Local GLP-1 production in the taste bud cells, the expression of GLP-1 receptor on the adjacent nerves, a functional continuum in the perception of sweet chemicals from the gut to the tongue and an identification of GLP-1 induced signaling pathways in peripheral and central gustatory coding all strongly suggest that GLP-1 is involved in the taste perception, especially sweet. However, the impact of GLP-1 based therapies on gustatory coding in humans remains largely unaddressed. Based on the molecular background we encourage further exploration of the tongue as a new treatment target for GLP-1 receptor agonists in clinical studies. Given that pharmacological manipulation of gustatory coding may represent a new potential strategy against obesity and diabetes, the topic is of utmost clinical relevance.
Advances in the Knowledge of the Underlying Airway Remodeling Mechanisms in Chronic Rhinosinusitis Based on the Endotypes: A Review
Authors: Lee Kijeong , Tai Junhu , Lee Sang Hag , Kim Tae Hoon
Published: January 2021

Chronic rhinosinusitis (CRS) is a chronic inflammatory condition of the nasal and paranasal sinus mucosa that affects up to 10% of the population worldwide. CRS is the most representative disease of the upper respiratory tract where airway remodeling occurs, including epithelial damage, thickening of the basement membrane, fibrosis, goblet cell hyperplasia, subepithelial edema, and osteitis. CRS is divided into two phenotypes according to the presence or absence of nasal polyps: CRS with nasal polyp (CRSwNP) and CRS without nasal polyps (CRSsNP). Based on the underlying pathophysiologic mechanism, CRS is also classified as eosinophilic CRS and non-eosinophilic CRS, owing to Type 2 T helper (Th2)-based inflammation and Type 1 T helper (Th1)/Type 17 T helper (Th17) skewed immune response, respectively. Differences in tissue remodeling in CRS are suggested to be based on the clinical phenotype and endotypes; this is because fibrosis is prominent in CRSsNP, whereas edematous changes occur in CRSwNP, especially in the eosinophilic type. This review aims to summarize the latest information on the different mechanisms of airway remodeling in CRS according to distinct endotypes.
Nail Melatonin Content: A Suitable Non-Invasive Marker of Melatonin Production
Authors: Gomez-Gomez Alex , Montero-San-Martin Blanca , Haro Noemí , Pozo Oscar J.
Published: January 2021

Melatonin plays multiple physiological roles in the human body. Evaluation of melatonin production by the determination of urinary 6-sulfatoxymelatonin in 24-h samples has important drawbacks which hinder the successful evaluation of melatonin production in large cohorts. Here, we evaluated the potential of nail analysis for estimating melatonin production. Firstly, mass spectrometry methodology for the determination of melatonin in nails was optimized and successfully validated. The method was found to be linear in the range 6.5–830 fg/mg with intraday and interday accuracy in the range 100–104 %, precision below 15 % and a LOD of 3.5 fg/mg. Secondly, nail melatonin concentrations from 84 volunteers (age 5–96) were determined. The expected correlation between melatonin and age was obtained (correlation coefficient −0.615; p < 0.001). Additionally, we showed that fingernails are preferable to toenails to determine nail melatonin content. Finally, fingernails collected for 180 days after melatonin administration (two volunteers, 1.9 mg/night during 5 days) were analyzed. Nail melatonin concentrations immediately rose after administration and went back to pre-administration values after ≈100 days in both volunteers. Our results suggest that melatonin determination in nails is a suitable non-invasive tool for the estimation of global melatonin production. Due to the easy collection and storage of nails, the long-term information obtained and the multiple functions of melatonin, nail melatonin content might complement dim light melatonin onset, which is commonly measured from plasma/saliva samples, paving the way for melatonin research.
Molecular Mechanisms of Periodontal Disease
Authors: Kajiya Mikihito , Kurihara Hidemi
Published: January 2021

Periodontal disease, one of the most prevalent human infectious diseases, is characterized by chronic inflammatory tissue destruction of the alveolar bone and the connective tissues supporting the tooth [...]
Heat Stress Responses and Thermotolerance in Maize
Authors: Li Zhaoxia , Howell Stephen H.
Published: January 2021

High temperatures causing heat stress disturb cellular homeostasis and impede growth and development in plants. Extensive agricultural losses are attributed to heat stress, often in combination with other stresses. Plants have evolved a variety of responses to heat stress to minimize damage and to protect themselves from further stress. A narrow temperature window separates growth from heat stress, and the range of temperatures conferring optimal growth often overlap with those producing heat stress. Heat stress induces a cytoplasmic heat stress response (HSR) in which heat shock transcription factors (HSFs) activate a constellation of genes encoding heat shock proteins (HSPs). Heat stress also induces the endoplasmic reticulum (ER)-localized unfolded protein response (UPR), which activates transcription factors that upregulate a different family of stress response genes. Heat stress also activates hormone responses and alternative RNA splicing, all of which may contribute to thermotolerance. Heat stress is often studied by subjecting plants to step increases in temperatures; however, more recent studies have demonstrated that heat shock responses occur under simulated field conditions in which temperatures are slowly ramped up to more moderate temperatures. Heat stress responses, assessed at a molecular level, could be used as traits for plant breeders to select for thermotolerance.
Oxidation of Hydrogen Sulfide by Quinones: How Polyphenols Initiate Their Cytoprotective Effects
Authors: Olson Kenneth R. , Gao Yan , Straub Karl D.
Published: January 2021

We have shown that autoxidized polyphenolic nutraceuticals oxidize H2S to polysulfides and thiosulfate and this may convey their cytoprotective effects. Polyphenol reactivity is largely attributed to the B ring, which is usually a form of hydroxyquinone (HQ). Here, we examine the effects of HQs on sulfur metabolism using H2S- and polysulfide-specific fluorophores (AzMC and SSP4, respectively) and thiosulfate sensitive silver nanoparticles (AgNP). In buffer, 1,4-dihydroxybenzene (1,4-DB), 1,4-benzoquinone (1,4-BQ), pyrogallol (PG) and gallic acid (GA) oxidized H2S to polysulfides and thiosulfate, whereas 1,2-DB, 1,3-DB, 1,2-dihydroxy,3,4-benzoquinone and shikimic acid did not. In addition, 1,4-DB, 1,4-BQ, PG and GA also increased polysulfide production in HEK293 cells. In buffer, H2S oxidation by 1,4-DB was oxygen-dependent, partially inhibited by tempol and trolox, and absorbance spectra were consistent with redox cycling between HQ autoxidation and H2S-mediated reduction. Neither 1,2-DB, 1,3-DB, 1,4-DB nor 1,4-BQ reduced polysulfides to H2S in either 21% or 0% oxygen. Epinephrine and norepinephrine also oxidized H2S to polysulfides and thiosulfate; dopamine and tyrosine were ineffective. Polyphenones were also examined, but only 2,5-dihydroxy- and 2,3,4-trihydroxybenzophenones oxidized H2S. These results show that H2S is readily oxidized by specific hydroxyquinones and quinones, most likely through the formation of a semiquinone radical intermediate derived from either reaction of oxygen with the reduced quinones, or from direct reaction between H2S and quinones. We propose that polysulfide production by these reactions contributes to the health-promoting benefits of polyphenolic nutraceuticals.
Cellular Redox State Acts as Switch to Determine the Direction of NNT-Catalyzed Reaction in Cystic Fibrosis Cells
Authors: Favia Maria , Atlante Anna
Published: January 2021

The redox states of NAD and NADP are linked to each other in the mitochondria thanks to the enzyme nicotinamide nucleotide transhydrogenase (NNT) which, by utilizing the mitochondrial membrane potential (mΔΨ), catalyzes the transfer of redox potential between these two coenzymes, reducing one at the expense of the oxidation of the other. In order to define NNT reaction direction in CF cells, NNT activity under different redox states of cell has been investigated. Using spectrophotometric and western blotting techniques, the presence, abundance and activity level of NNT were determined. In parallel, the levels of NADPH and NADH as well as of mitochondrial and cellular ROS were also quantified. CF cells showed a 70% increase in protein expression compared to the Wt sample; however, regarding NNT activity, it was surprisingly lower in CF cells than healthy cells (about 30%). The cellular redox state, together with the low mΔΨ, pushes to drive NNT reverse reaction, at the expense of its antioxidant potential, thus consuming NADPH to support NADH production. At the same time, the reduced NNT activity prevents the NADH, produced by the reaction, from causing an explosion of ROS by the damaged respiratory chain, in accordance with the reduced level of mitochondrial ROS in NNT-loss cells. This new information on cellular bioenergetics represents an important building block for further understanding the molecular mechanisms responsible for cellular dysfunction in cystic fibrosis.
Structural Reorganization of Imidazolium Ionic Liquids Induced by Pressure-Enhanced Ionic Liquid—Polyethylene Oxide Interactions
Authors: Wang Teng-Hui , Hsu Li-Wen , Chang Hai-Chou
Published: January 2021

Mixtures of polyethylene oxide (PEO, M.W.~900,000) and imidazolium ionic liquids (ILs) are studied using high-pressure Fourier-transform infrared spectroscopy. At ambient pressure, the spectral features in the C–H stretching region reveal that PEO can disturb the local structures of the imidazolium rings of [BMIM]+ and [HMIM]+. The pressure-induced phase transition of pure 1-butyl-3-methylimidazolium bromide ([BMIM]Br) is observed at a pressure of 0.4 GPa. Pressure-enhanced [BMIM]Br-PEO interactions may assist PEO in dividing [BMIM]Br clusters to hinder the aggregation of [BMIM]Br under high pressures. The C–H absorptions of pure 1-hexyl-3-methylimidazolium bromide [HMIM]Br do not show band narrowing under high pressures, as observed for pure [BMIM]Br. The band narrowing of C–H peaks is observed at 1.5 GPa for the [HMIM]Br-PEO mixture containing 80 wt% of [HMIM]Br. The presence of PEO may reorganize [HMIM]Br clusters into a semi-crystalline network under high pressures. The differences in aggregation states for ambient-pressure phase and high-pressure phase may suggest the potential of [HMIM]Br-PEO (M.W.~900,000) for serving as optical or electronic switches.
Aspects Regarding Safety and Security in Hotels: Romanian Experience
Authors: Anichiti Alexandru , Dragolea Larisa-Loredana , Tacu Hârșan Georgia-Daniela , Haller Alina-Petronela , Butnaru Gina Ionela
Published: January 2021

This study investigates safety and security from the perspective of Romanian tourists by assessing the level of importance that tourists give to safety and security depending on the level of classification of the services they experienced and the generation to which they belong. We used a quantitative research method in the form of a questionnaire and analysed eight dimensions of safety and security: detectors, emergency preparedness, medical preparedness, staff security, guestroom security, pool and beach security, hotel access control, and cyber security. We identified the differences between tourists’ perception of safety and security depending on the level of classification of accommodation services and on generation.
Morphology and Distribution of Antennal Sensilla in Three Species of Thripidae (Thysanoptera) Infesting Alfalfa Medicago sativa
Authors: Liu Yan-Qi , Li Jin , Ban Li-Ping
Published: January 2021

Thrips are important pests to alfalfa Medicago sativa. Similar as many other plant-feeding insects, thrips rely on the antennae to receive chemical signals in the environment to locate their hosts. Previous studies indicated that sensilla of different shapes on the surface of insect antenna play an important role in signal recognition. However, morphological analysis of the antennal sensilla in Thysanoptera has been limited to only a few species. To expand the understanding of how antennal sensilla are related to semiochemical detection in thrips, here we compared the morphology and distribution of antennal sensilla in three thrip species, Odontothrips loti, Megalurothrips distalis, and Sericothrips kaszabi, by scanning electron microscope (SEM). The antennae of these three species are all composed of eight segments and share similar types of sensilla which distribute similarly in each segment, despite that their numbers show sexual dimorphism. Specifically, nine major types of sensilla in total were found, including three types of sensilla basiconica (SBI, SBII, and SBIII), two types of sensilla chaetica (SChI and SChII), and one type for each of sensilla coeloconica (SCo), sensilla trichodea (ST), sensilla campaniformia (SCa), and sensilla cavity (SCav). The potential functions of sensilla were discussed according to the previous research results and will lay a morphological foundation for the study of the olfactory mechanism of three species of thrips.