bluuebunny/crossref_metadata_2025 huggingface dataset:
ChemR23 activation reprograms macrophages toward a less inflammatory phenotype and dampens carcinoma progression
Authors: Lavy Margot , Gauttier Vanessa , Dumont Alison , Chocteau Florian , Deshayes Sophie , Fresquet Judith , Dehame Virginie , Girault Isabelle , Trilleaud Charlène , Neyton Stéphanie , Mary Caroline , Juin Philippe , Poirier Nicolas , Barillé-Nion Sophie , Blanquart Christophe
Published: July 2023

Introduction

Tumor Associated Macrophages (TAM) are a major component of the tumor environment and their accumulation often correlates with poor prognosis by contributing to local inflammation, inhibition of anti-tumor immune response and resistance to anticancer treatments. In this study, we thus investigated the anti-cancer therapeutic interest to target ChemR23, a receptor of the resolution of inflammation expressed by macrophages, using an agonist monoclonal antibody, αChemR23.

Methods

Human GM-CSF, M-CSF and Tumor Associated Macrophage (TAM)-like macrophages were obtained by incubation of monocytes from healthy donors with GM-CSF, M-CSF or tumor cell supernatants (Breast cancer (BC) or malignant pleural mesothelioma (MPM) cells). The effects of αChemR23 on macrophages were studied at the transcriptomic, protein and functional level. Datasets from The Cancer Genome Atlas (TCGA) were used to study CMKLR1 expression, coding for ChemR23, in BC and MPM tumors. In vivo, αChemR23 was evaluated on overall survival, metastasis development and transcriptomic modification of the metastatic niche using a model of resected triple negative breast cancer.

Results

We show that ChemR23 is expressed at higher levels in M-CSF and tumor cell supernatant differentiated macrophages (TAM-like) than in GM-CSF-differentiated macrophages. ChemR23 activation triggered by αChemR23 deeply modulates M-CSF and TAM-like macrophages including profile of cell surface markers, cytokine secretion, gene mRNA expression and immune functions. The expression of ChemR23 coding gene (CMKLR1) strongly correlates to TAM markers in human BC tumors and MPM and its histological detection in these tumors mainly corresponds to TAM expression. In vivo, treatment with αChemR23 agonist increased mouse survival and decreased metastasis occurrence in a model of triple-negative BC in correlation with modulation of TAM phenotype in the metastatic niche.

Conclusion

These results open an attractive opportunity to target TAM and the resolution of inflammation pathways through ChemR23 to circumvent TAM pro-tumoral effects.
Commonalities between the Atacama Desert and Antarctica rhizosphere microbial communities
Authors: Contreras María José , Leal Karla , Bruna Pablo , Nuñez-Montero Kattia , Goméz-Espinoza Olman , Santos Andrés , Bravo León , Valenzuela Bernardita , Solis Francisco , Gahona Giovanni , Cayo Mayra , Dinamarca M. Alejandro , Ibacache-Quiroga Claudia , Zamorano Pedro , Barrientos Leticia
Published: July 2023

Plant-microbiota interactions have significant effects on plant growth, health, and productivity. Rhizosphere microorganisms are involved in processes that promote physiological responses to biotic and abiotic stresses in plants. In recent years, the interest in microorganisms to improve plant productivity has increased, mainly aiming to find promising strains to overcome the impact of climate change on crops. In this work, we hypothesize that given the desertic environment of the Antarctic and the Atacama Desert, different plant species inhabiting these areas might share microbial taxa with functions associated with desiccation and drought stress tolerance. Therefore, in this study, we described and compared the composition of the rhizobacterial community associated with Deschampsia antarctica (Da), Colobanthus quitensis (Cq) from Antarctic territories, and Croton chilensis (Cc), Eulychnia iquiquensis (Ei) and Nicotiana solanifolia (Ns) from coastal Atacama Desert environments by using 16S rRNA amplicon sequencing. In addition, we evaluated the putative functions of that rhizobacterial community that are likely involved in nutrient acquisition and stress tolerance of these plants. Even though each plant microbial rhizosphere presents a unique taxonomic pattern of 3,019 different sequences, the distribution at the genus level showed a core microbiome with a higher abundance of Haliangium, Bryobacter, Bacillus, MND1 from the Nitrosomonadaceae family, and unclassified taxa from Gemmatiamonadaceae and Chitinophagaceae families in the rhizosphere of all samples analyzed (781 unique sequences). In addition, species Gemmatirosa kalamazoonesis and Solibacter usitatus were shared by the core microbiome of both Antarctic and Desert plants. All the taxa mentioned above had been previously associated with beneficial effects in plants. Also, this microbial core composition converged with the functional prediction related to survival under harsh conditions, including chemoheterotrophy, ureolysis, phototrophy, nitrogen fixation, and chitinolysis. Therefore, this study provides relevant information for the exploration of rhizospheric microorganisms from plants in extreme conditions of the Atacama Desert and Antarctic as promising plant growth-promoting rhizobacteria.
A root-knot nematode effector manipulates the rhizosphere microbiome for establishing parasitism relationship with hosts
Authors: Liu Rui , Chen Mengfei , Liu Boliang , Huang Kaiwei , Mao Zhenchuan , Li Huixia , Zhao Jianlong
Published: July 2023

Introduction

Root-knot nematode (RKN; Meloidogyne spp.) is one of the most infamous soilborne plant diseases, causing severe crop losses every year. Effector proteins secreted by RKNs play crucial roles during plant-nematode interaction. However, less is known about whether RKN effector proteins can impact the rhizosphere microbial environment.

Methods

In this study, we investigated the rhizosphere microbiome community of MiMIF-2 (a plant immunity-modulating effector) transgenic Arabidopsis thaliana with or without nematode infection using the Illumina high-throughput sequencing analysis.

Results and discussion

The results showed that the bacterial species richness index increased, while the fungi species richness index decreased in M. incognita-infected MiMIF-2 transgenic A. thaliana plants. The relative abundance of genera such as Clitopilus, Komagataeibacter, Lactobacillus, Prevotella, Moritella, Vibrio, Escherichia-Shigella, and Pseudomonas was reduced in MiMIF-2 transgenic A. thaliana plants compared to wild type, but was significantly increased after inoculation with M. incognita. The Cluster of Orthologous Genes (COG) function classification analysis revealed a decrease in the relative abundance of defense mechanisms, secondary metabolite biosynthesis, transport, and nematode infection catabolism-related functions in MiMIF-2 lines compared to the wild type. These differences may be the reason for the increased susceptibility of MiMIF-2 transgenic A. thaliana to nematode infection. Our results provide a new insight into RKN effector proteins and their association with the microbial community, host, and plant pathogens, which will lead to the exploration of new innovative ideas for future biological control of RKNs.
Dynamics of PO2 and VO2 in resting and contracting rat spinotrapezius muscle
Authors: Golub Aleksander S. , Song Bjorn K. , Nugent William H. , Pittman Roland N.
Published: July 2023

This study examined changes in interstitial PO2, which allowed calculation of VO2 during periods of rest, muscle contraction and recovery using an in situ rat spinotrapezius muscle preparation. The PO2 was measured using phosphorescence quenching microscopy and the muscle VO2 was calculated as the rate of O2 disappearance during brief periods of muscle compression to stop blood flow with a supra-systolic pressure. The PO2 and VO2 measurements were made during “5 s compression and 15 s recovery” (CR) cycles. With all three stimulation frequencies, 1, 2 and 4 Hz, the fall in interstitial PO2 and rise in VO2 from resting values occurred within the first 20 s of contraction. The PO2 during contraction became lower as stimulation frequency increased from 1 to 4 Hz. VO2 was higher at 2 Hz than at 1 Hz contraction. With cessation of stimulation, PO2 began increasing exponentially towards baseline values. After 1 and 2 Hz contraction, the fall in muscle VO2 was delayed by one CR cycle and then exponentially decreased towards resting values. After 4 Hz stimulation, VO2 increased for 2 cycles and then decreased. The post-contraction transients of PO2 and VO2 were not synchronous and had different time constants. With further analysis two distinct functional responses were identified across all stimulation frequencies having PO2 during contraction above or below 30 mmHg. The corresponding VO2 responses were different - for “high” PO2, muscle VO2 reached high levels, while for the “low” PO2 data set muscle VO2 remained low. Recovery patterns were similar to those described above. In summary, local microscopic PO2 and VO2 were measured in resting and contracting muscle in situ and the post-contraction transients of PO2 and VO2 were all much slower than the onset transients.
Using the photoplethysmography method to monitor age-related changes in the cardiovascular system
Authors: Djurić Biljana , Žikić Katarina , Nestorović Zorica , Lepojević-Stefanović Danijela , Milošević Nebojša , Žikić Dejan
Published: July 2023

Introduction: Aging is a physiological process characterized by progressive changes in all organ systems. In the last few decades, the elderly population has been growing, so the scientific community is focusing on the investigation of the aging process, all in order to improve the quality of life in elderly. One of the biggest challenges in studying the impact of the aging on the human body represents the monitoring of the changes that inevitably occur in arterial blood vessels. Therefore, the medical community has invested a great deal of effort in studying and discovering new methods and tools that could be used to monitor the changes in arterial blood vessels caused by the aging process. The goal of our research was to develop a new diagnostic method using a photoplethysmographic sensor and to examine the impact of the aging process on the cardiovascular system in adults. Long-term recorded arterial blood flow waveforms were analyzed using detrended fluctuation analysis.

Materials and Methods: The study included 117 respondents, aged 20–70 years. The waveform of the arterial blood flow was recorded for 5 min, with an optical sensor placed above the left common carotid artery, simultaneously with a single-channel ECG. For each cardiac cycle, the blood flow amplitude was determined, and a new time series was formed, which was analyzed non-linearly (DFA method). The values of the scalar coefficients α1 and α2, particularly their ratio (α1/α2) were obtained, which were then monitored in relation to the age of the subjects.

Result: The values of the scalar ratio (α1/α2) were significantly different between the subjects older and younger than 50 years. The value of the α1/α2 decreased exponentially with the aging. In the population of middle-aged adults, this ratio had a value around 1, in young adults the value was exclusively higher than 1 and in older adults the value was exclusively lower than 1.

Conclusion: The results of this study indicated that the aging led to a decrease in the α1/α2 in the population of healthy subjects. With this non-invasive method, changes in the cardiovascular system due to aging can be detected and monitored.
Seasonal changes in the abundance Fusarium proliferatium, microbial endophytes and nutrient levels in the roots of hybrid bamboo Bambusa pervariabilis × Dendrocalamopsis grandis
Authors: Li Lin , Wang Yaxuan , Yu Cailin , Li Shuying , Lin Tiantian , Han Shan , Zhu Tianhui , Li Shujiang
Published: July 2023

Plant root pathogens invade the soil around plant roots, disturbing the systemic balance, reducing plant defenses, and causing severe disease. At present, there are few studies on the severity of plant diseases caused by pathogen invasion in different seasons and how pathogens affect root microecology. In this study, we compared the levels of nutrients in the root tissues of the two groups of plants. We used 16S and ITS amplicon sequencing with Illumina NovaSeq 6000 to compare seasonal changes in the composition and structure of microbial communities from healthy roots of bamboo Bambusa pervariabilis × Dendrocalamopsis grandis and roots infected by the soilborne pathogen Fusarium proliferatum. We have found that the invasion of the pathogen led to a substantial decrease in nutrient elements in bamboo roots, except for nitrogen. The pathogen presence correlated with seasonal changes in the bamboo root microbiome and decreased bacterial richness in diseased plants. The root microbial community structure of healthy plants was more stable than that of their diseased counterparts. Furthermore, we identified the lesion area and relative abundance of F. proliferatum were significant predictors of disease progression. The potassium tissue content and the disease lesion area were identified as factors linked with the observed changes in the bamboo root microbiome. This study provides a theoretical foundation for understanding the seasonal dynamics F. proliferatum, an economically important soilborne pathogen of hybrid bamboo grown in Sichuan Province, China.
Metabolic changes and potential biomarkers in "Candidatus Liberibacter solanacearum"-infected potato psyllids: implications for psyllid-pathogen interactions
Authors: Li Yelin , Tan Zhiqing , Wang Xiaolan , Hou Liping
Published: July 2023

Psyllid yellows, vein-greening (VG), and zebra chip (ZC) diseases, which are primarily transmitted by potato psyllid (PoP) carrying Candidatus Liberibacter solanacearum (CLso), have caused significant losses in solanaceous crop production worldwide. Pathogens interact with their vectors at the organic and cellular levels, while the potential changes that may occur at the biochemical level are less well reported. In this study, the impact of CLso on the metabolism of PoP and the identification of biomarkers from infected psyllids were examined. Using ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) analysis, metabolomic changes in CLso-infected psyllids were compared to uninfected ones. A total of 34 metabolites were identified as potential biomarkers of CLso infection, which were primarily related to amino acid, carbohydrate, and lipid metabolism. The significant increase in glycerophospholipids is thought to be associated with CLso evading the insect vector’s immune defense. Matrix-assisted Laser Desorption Ionization Mass Spectrometry Imaging (MALDI-MSI) was used to map the spatial distribution of these biomarkers, revealing that 15-keto-Prostaglandin E2 and alpha-D-Glucose were highly expressed in the abdomen of uninfected psyllids but down-regulated in infected psyllids. It is speculated that this down-regulation may be due to CLso evading surveillance by immune suppression in the PoP midgut. Overall, valuable biochemical information was provided, a theoretical basis for a better understanding of psyllid-pathogen interactions was offered, and the findings may aid in breaking the transmission cycle of these diseases.
Assessment of nematicidal and plant growth-promoting effects of Burkholderia sp. JB-2 in root-knot nematode-infested soil
Authors: Kim Jong-Hoon , Lee Byeong-Min , Kang Min-Kyoung , Park Dong-Jin , Choi In-Soo , Park Ho-Yong , Lim Chi-Hwan , Son Kwang-Hee
Published: July 2023

Root-knot nematodes (RKN), Meloidogyne spp., are plant-parasitic nematodes that are responsible for considerable economic losses worldwide, because of the damage they cause to numerous plant species and the inadequate biological agents available to combat them. Therefore, developing novel and eco-friendly nematicides is necessary. In the present study, Burkholderia sp. JB-2, isolated from RKN-infested rhizosphere soil in South Korea, was evaluated to determine its nematicidal and plant growth-promoting effects under in vitro and in vivo conditions. Cell-free filtrates of the JB-2 strain showed high levels of nematicidal activity against second-stage juveniles (J2) of M. incognita, with 87.5% mortality following two days of treatment. In addition, the assessment of the activity against other six plant parasitic nematodes (M. javanica, M. hapla, M. arenaria, Ditylenchus destructor, Aphelenchoides subtenuis, and Heterodera trifolii) showed that the cell-free filtrates have a broad nematicidal spectrum. The three defense-responsive (MiMIF-2, MiDaf16-like1, and MiSkn1-like1) genes were activated, while Mi-cm-3 was downregulated when treated with cell-free filtrates of JB-2 cultures on J2. The greenhouse experiments suggested that the cell-free filtrates of the JB-2 strain efficiently controlled the nematode population in soil and egg mass formations of M. incognita in tomato (Solanum lycopersicum L., cv. Rutgers). An improvement in the host plant growth was observed, in which the shoot length and fresh weights of shoots and roots increased. The treatment with 10% of JB-2 cell-free filtrates significantly upregulated the expression levels of plant defenses (SlPR1, SlPR5, and SlPAL) and growth-promoting (ACO1, Exp18, and SlIAA1) genes compared with the corresponding parameters of the control group. Therefore, JB-2 could be a promising candidate for the sustainable management of RKN.
Adolescent mentalizing and childhood emotional abuse: implications for depression, anxiety, and borderline personality disorder features
Authors: Martin-Gagnon Gabriel , Normandin Lina , Fonagy Peter , Ensink Karin
Published: July 2023

Background

There is preliminary evidence that childhood emotional abuse (CEA) is a risk factor for adolescent mentalizing difficulties (Uncertainty/Confusion about mental states) and borderline personality features and that Uncertainty/Confusion about mental states mediate the relationship between CEA and adolescent borderline personality features, but these findings need replication. Furthermore, no previous studies have examined the relationship between adolescent mentalizing deficits, anxiety, and depression in the context of CEA.

Objectives

This study examined the associations between CEA, adolescent borderline personality features, depression and anxiety symptoms and tested a pathway model where Uncertainty/Confusion about mental states mediates the relationships between CEA and adolescent borderline personality features, depression and anxiety symptoms.

Method

A clinical sample of 94 adolescents completed the Reflective Function Questionnaire for Youth (RFQY) to assess mentalizing, the Childhood Experiences of Care and Abuse Questionnaire (CECA-Q), the Borderline Personality Disorders Features Scale (BPFS-C), and the Beck Youth Inventories for Depression (BDI-Y) and Anxiety (BAI-Y).

Results

Uncertainty/Confusion about mental states partially mediated the relationship between CEA and borderline traits as well as anxiety. In addition, there was an indirect effect where CEA predicted Uncertainty/Confusion about mental states, which then predicted depression.

Discussion

The findings are consistent with the mentalizing model of psychopathology and provide new evidence that Uncertainty/Confusion about mental states might be a critical mentalizing deficit that characterizes the associations between CEA and adolescent BPD features and depression and anxiety symptoms. Uncertainty/Confusion may be a transdiagnostic risk factor for adolescent psychological distress and dysfunction. We discuss the clinical implications.
Physical and mental health conditions account for variability in awareness of age-related changes
Authors: Sabatini Serena , Turner Shelbie , Brooker Helen , Ballard Clive , Corbett Anne , Hampshire Adam
Published: July 2023

Background

The concept of Awareness of Age-Related Changes captures people’s perceptions of the positive (AARC-gains) and negative (AARC-losses) age-related changes they experience in several life domains, including their health. We investigated the cross-sectional associations of number and type of physical and mental health conditions with AARC-gains and AARC-losses.

Methods

The sample comprised 3,786 middle-aged and older adults (mean age = 67.04 years; SD = 6.88) participating to the UK PROTECT study. We used hierarchical regression models to analyze whether after having included sociodemographic variables (model 1), number of physical (model 2) and of mental (model 3) health conditions explained a significant additional amount of variance in AARC-gains and AARC-losses, and whether the association between number of conditions and AARC depended on participants’ age. We used multiple regression models to analyze the associations of types of physical and mental health conditions with AARC-gains and AARC-losses.

Results

A higher number of physical health conditions was associated with higher AARC-gains and higher AARC-losses, but the association did not depend on participant age. After controlling for the number of physical health conditions, a higher number of mental health conditions was associated with higher AARC-losses but not with AARC-gains, and the association was stronger among older participants. Small effects were found between greater AARC-gains and current cancer and between greater AARC-losses and diagnoses of mild cognitive impairment, Parkinson’s disease, arthritic condition, cancer in full remission, osteoporosis, depression, anxiety disorders, and personality disorder. The remaining health conditions were either negligibly or non-statistically related to AARC-losses.

Conclusion

Middle-aged and older adults having more physical health conditions and more mental health conditions may be at higher risk of negative views on their own aging. However, specific physical health conditions, such as arthritis, and certain mental health conditions, such as depression, may make adults particularly vulnerable to negative age-related perceptions.
Case report: Psychosis and catatonia in an adolescent patient with adipsic hypernatremia and autoantibodies against the subfornical organ
Authors: Liebrand Matthias , Rebsamen Michael , Nakamura-Utsunomiya Akari , von den Driesch Luisa , Köck Patrick , Caccia Julien , Hamann Christoph , Wiest Roland , Kaess Michael , Walther Sebastian , Tschumi Sibylle , Hiyama Takeshi Y. , Kindler Jochen
Published: July 2023

This is the first description of a patient in which adipsic hypernatremia, a rare autoimmune encephalitis, presented in combination with complex psychiatric symptomatology, including psychosis and catatonia. Adipsic hypernatremia is characterized by autoantibodies against the thirst center of the brain. These autoantibodies cause inflammation and apoptosis in key regions of water homeostasis, leading to lack of thirst and highly increased serum sodium. To date, the symptoms of weakness, fatigue and drowsiness have been associated with adipsic hypernatremia, but no psychiatric symptomatology. Here, we showcase the first description of an adolescent patient, in which severe and complex psychiatric symptoms presented along with adipsic hypernatremia. The patient experienced delusion, hallucinations, restlessness and pronounced depression. Further, he showed ritualized, aggressive, disinhibited and sexualized behavior, as well as self-harm and psychomotor symptoms. Due to his severe condition, he was hospitalized on the emergency unit of the child and adolescent psychiatry for 8 months. Key symptoms of the presented clinical picture are: childhood-onset complex and treatment-resistant psychosis/catatonia, pronounced behavioral problems, fatigue, absent thirst perception, hypernatremia and elevated prolactin levels. This case report renders first evidence speaking for a causal link between the autoimmune adipsic hypernatremia and the psychotic disorder. Moreover, it sheds light on a new form of autoimmune psychosis.
Factors affecting decreased physical activity during the COVID-19 pandemic: an age-, gender-, and body mass index-matched study
Authors: Shin Jhin-Yi , Lee Jaemoo , Lee Jung-Min , Ho Nam Yoon
Published: July 2023

Purpose

This study aims to investigate the association between factors affecting decreased physical activity (PA) during the COVID-19 pandemic by matching groups based on age, gender, and BMI variables using public Community Health Survey (CHS) data.

Methods

Data from the CHS was selected and used to investigate health-related factors related to PA, including demographic, psychological, behavioral characteristics, sociocultural, and chronic disease. Exact group matching was conducted based on age, gender, and BMI variables. Frequency analysis, Chi-square test (χ2 test), and multinominal logistic regression analysis were performed to analyze the data, and odds ratio (OR) and 95% confidence interval (95% CI) were presented. The study also examined the impact of COVID-19 on PA, the fear of PA infection.

Results

The logistic regression analysis by gender showed that PA decreased in all age groups, males, and females during the COVID-19 pandemic. The decrease in PA was lower in age groups other than those aged 60 or older. Stress experience, residence area, housing type, drinking, smoking, education level, and fear of infection were found to affect decreased PA due to COVID-19. Specifically, experiencing stress (Odds Ratio [OR] = 1.178; 95% Confidence Interval [CI] = 1.054 ~ 1.317) and increased smoking (OR = 1.332; 95% CI = 1.073 ~ 1.653) had a slightly higher impact on decreased PA. Conversely, living in a suburban area (OR = 0.653; CI = 0.585 ~ 0.728), having public housing (OR = 0.836; CI = 0.754 ~ 0.928), having less than a high school education (OR = 0.813; CI = 0.729 ~ 0.907), staying the same with alcohol (OR = 0.567; CI = 0.507 ~ 0.633) and smoking (OR = 0.836; CI = 0.728 ~ 0.959), and having low fear of infection (OR = 0.817; CI = 0.737 ~ 0.905) had a slightly lower impact on decreased PA.

Conclusion

PA should be maintained or increased, particularly in the context of social distancing measures during the pandemic. To ensure that PA can be sustained, a program should be developed that considers the individual’s geographical location, economic status, lifestyle, and environment.
Socio-cultural, economic, and environmental implications for innovation in sustainable food in Africa
Authors: Assan Never
Published: July 2023

The debate over innovation in sustainable food in Africa is multifaceted and complex since it is impacted by an array of issues, including social, cultural, religious, and environmental implications. The diversity of cultures on the continent, as well as religion, influence the foods that people eat. The architecture of agro-ecological zones influences food production and, as a result, the sort of innovation in sustainable food. Africa, in particular, has immense potential for the development of multiple sustainable food innovations, whether in terms of food, culture, or tradition. By sustainable food innovation, it may be possible to improve Africa’s food systems from the demand side while also mitigating the effects of climate change. For Africa, it is the food crisis that simply cannot be allowed to go to waste, hence the need for innovation in sustainable food solutions in its own context. Africa’s long-standing food crisis, recently worsened by the COVID-19 pandemic and, increasingly, by climate change and heavily weighed on gender disparities in agriculture and food production, threatens to have dire consequences, especially on achieving the SDGs in Africa. Innovation in sustainable food is at the forefront of all Africa needs for its survival at every current stage of population evolution; this applies whether in industrialized or impoverished nations, rural or urban, village or metropolis, etc. It is false to believe that the only sources of innovation in sustainable food are cutting-edge research or the most recent developments in genomic science. The discussion gives an opinion on the socio-cultural, economic, and environmental factors and their implications for innovation in sustainable food in Africa.
Fly Bioash Ameliorates Acid Luvisol and Increases Sunflower (Helianthus annuus L.) Yield in Field Conditions without Compromising the Risk of Radioactive Contamination
Authors: Ondrasek Gabrijel , Kranjčec Filip , Horvatinec Jelena , Bubalo Kovačić Marina , Husnjak Stjepan , Čoga Lepomir , Babić Dinko , Rašeta Davor , Volarić Nikola , Fulajtar Emil , Rashid Muhammad Imtiaz , Včev Aleksandar , Petrinec Branko
Published: July 2023

Fly bioash (FBA) as a by-product of biomass-fuelled facilities exhibits alkaline properties and is enriched with phytonutrients, thereby offering the potential to effectively ameliorate acidic and nutrient-deficient soils. However, concerns about health risks due to a potential FBA radioactive contamination are still not well studied, notably under field conditions. This study examined pH changes and concentrations of natural (238U, 232Th, 226Ra, 40K) and anthropogenic (137Cs) radionuclides after application of very alkaline (pH > 12) FBA in: (i) highly acid (pHKCl = 4.1) Luvisol and (ii) sunflower (Helianthus annuus L.) seeds, grown in organic farming and rain-fed conditions. FBA (originated from a modern cogeneration, fuelled on certified deciduous forest wood chips) was applied at increasing doses; 0, 4.5, 8.6, 13, and 17.2 t/ha. After 54 months of application, FBA significantly increased soil pHKCl by up to 1.8 unit and the seed yield by 15%, compared with no amended Control, without compromising soil electrical conductivity (salinity). The activity concentrations (Ac) of all observed radionuclides, measured using high-resolution gamma-ray spectrometry, were not altered under FBA application, neither in the surface (0–30 cm) Luvisol horizon nor in the sunflower seed. Moreover, the Ac of 238U, 232Th, and 137Cs in the seed were below detection limit, whereas the Ac of 40K and 226Ra were lower by up to 2.6 and 61 times, respectively, than their corresponding Ac in the soil treatments. The radiological footprint of FBA exhibited lower Ac for most of the observed radionuclides compared with both (i) Croatian non-arable topsoils (with reductions of 238U 3.6 times, 232Th 1.8 times, 226Ra 1.7 times, and 137Cs 1.5 times) and (ii) widely used mineral N/P/K fertilisers in conventional agroecosystems (with reductions of 238U 12.5 times; 226Ra 1.3 times, and 40K 2.4 times). Our findings provide evidence that the application of FBA as a soil conditioner does not pose radiological health or environmental risks, contributing to more sustainable agri-food production and circular bioeconomy. However, it is essential to conduct further studies to comprehensively investigate the effects of FBA application on soil and crop quality across diverse environmental conditions and extended spatiotemporal scales.
The Effect of Dietary Synbiotics in Actively Racing Standardbred Horses Receiving Trimethoprim/Sulfadiazine
Authors: Lagounova Maria , MacNicol Jennifer L. , Weese J. Scott , Pearson Wendy
Published: July 2023

Synbiotics are often provided to horses receiving antibiotics to protect against microbiome disturbances, despite a lack of evidence for efficacy. The purpose of this study was to evaluate the effect of a synbiotic product in horses receiving antibiotics. Sixteen actively racing Standardbred horses were randomly allocated (four-way crossover) to one of four groups: antibiotics (10 days; AB), synbiotics (28 days; PROBIOPlusTM; PBP), PBP + AB, or Control. The fecal microbiome was investigated using 16S rRNA sequencing, and fecal dry matter (DM; %), pH, and scores (FS; 0–9) were measured. Data were analyzed with two-way ANOVA. Results found microbiota differences in community membership between PBP + AB and all other treatments during and after antibiotic treatment. During antibiotic treatment, AB and PBP + AB were significantly different from Control. After antibiotic treatment, PBP + AB was significantly different from all other treatments. The few differences found in relative abundance of phyla or predominant genera were mostly in fiber degrading bacteria. The Fibrobacter population was significantly higher in AB and PBP + AB horses than Control. Unclassified Ruminococcaceae was significantly higher in Control than AB and PBP. After antibiotic treatment, PBP + AB horses were significantly higher than PBP horses. In conclusion, these data provide support for the ability of PROBIOPlus™ to maintain healthy gastrointestinal microbiome during antibiotic treatment.
Intelligent Identification and Verification of Flutter Derivatives and Critical Velocity of Closed-Box Girders Using Gradient Boosting Decision Tree
Authors: Chen Neyu , Ge Yaojun , Borri Claudio
Published: July 2023

Flutter derivatives (FDs) of the bridge deck are basic aerodynamic parameters by which flutter analysis determines critical flutter velocity (CFV), and they are traditionally identified by sectional model wind tunnel tests or computational fluid dynamics (CFD) numerical simulation. Based on some wind tunnel testing results and numerical simulation data, the machine learning models for identifying FDs of closed-box girders are trained and developed via a gradient boosting decision tree in this study. The models can explore the underlying input–output transfer relationship of datasets and realize rapid intelligent identification of FDs without wind tunnel tests or numerical simulation. This method also provides a convenient and feasible option for expanding datasets of FDs, and the distribution of FDs can be analyzed through the post-interpretation of trained models. Combined with FD sensitivity analysis, the models can be verified by the calculation error of CFV. In addition, the proposed method can help determine the appropriate shape of the box girder cross-section in the preliminary design stage of long-span bridges and provide the necessary reference for aerodynamic shape optimization by modifying the local geometric features of the cross-section.
Neutrophil–Lymphocyte Ratio as a Predictor of Cerebral Small Vessel Disease in a Geriatric Community: The I-Lan Longitudinal Aging Study
Authors: Chuang Shao-Yuan , Hsu Yin-Chen , Chou Kuang-Wei , Chang Kuo-Song , Wong Chiong-Hee , Hsu Ya-Hui , Cheng Hao-Min , Chen Chien-Wei , Chen Pang-Yen
Published: July 2023

Cerebral Small Vessel Disease (CSVD) frequently affects the elderly, with inflammation playing a crucial role in related health complications, including dementia, stroke, and SVD. Studies, including animal experiments, indicate a strong link between inflammation and SVD progression. The Neutrophil-Lymphocyte Ratio (NLR) serves as a possible biomarker for ongoing inflammatory risks. A total of 720 adults aged 50 years or older from the community-based I-Lan Longitudinal Aging Study were included in this study. General linear regression and ordinally logistic regression analyses were performed to evaluate the association between NLR and CSVD. We further examined the presence of lacune, microbleed, and white matter hyperintensity (WMH) on brain MRI, which were used to construct a combined CSVD score. The NLR was positively associated with WMH (adjusted r = 0.109, p = 0.003), microbleed (adjusted r = 0.102, p = 0.006), and lacune (adjusted r = 0.100, p = 0.008). After adjustments for smoking, drinking, and physical activity in the ordinal logistic regression analysis, age, gender, brachial Systolic Blood Pressure (SBP), fasting glucose, LDL-cholesterol, and Hs-CRP were compared among subjects with low tertile (T1), medium tertile (T2) and high tertile (T3) NLR. The results showed that T2 vs. T1 had an odds ratio of 1.23 (0.86–1.77); and T3 vs. T1 had an odds ratio of 1.87 (1.29–2.71) of CSVD scores in four groups (zero (reference group), one, two, and three or more). NLR could be used to assess the state of inflammation in cerebral vessels. A significant and positive correlation between NLR and CSVD was verified in this study. However, the practical clinical application of NLR in CSVD patients and prognosis prediction should be validated through more scientific attempts.
Empirical Investigation of How Social Media Usage Enhances Employee Creativity: The Role of Knowledge Management Behavior
Authors: Zhang Huiqin , Wang Meng , Chen Anhang
Published: July 2023

Employee use of social media in the workplace has become a common phenomenon. Thus, how to effectively manage and utilize employee social media usage in the workplace has become a new issue. This study examines how employees’ work-related and social-related social media usage at work can present different impacts upon their creativity through knowledge management behaviors. To test the research model, this study collected data from 425 employees in various industries in China and utilized a covariance-based structural equation model (CB-SEM) to test the hypotheses. The results suggested that work-related social media usage enhances employee creativity through promoting knowledge sharing and restraining knowledge manipulation. On the contrary, social-related social media usage cannot indirectly influence employee creativity through knowledge management behaviors. This study contributes to the literature on social media research by providing theoretical arguments on how employee use of social media for different purposes affects their creativity. Furthermore, this research offers the insight of the different paths of work-related and social-related social media usage that influence employee creativity rather than treating social media usage as a unitary concept and linking it simply with work results. This study also explores the role of three knowledge management behaviors in the relationship between social media usage and employee creativity.
A Sectorial Validation and Application of a Conceptual Framework for Creating a Brand Management Strategy
Authors: Cid Allan , Blanchet Pierre , Robichaud François , Kinuani Nsimba
Published: July 2023

Brands can be one of a company’s most valuable intangible assets and a lever to generate value. As a source of added value, a brand should be strategically built and managed. To fully take advantage of the benefits that the brand provides, it is necessary to propose a brand management strategy. A conceptual framework was developed by the authors as an alternative to propose a brand management strategy according to a specific business scenario. The objective of this study is to validate this conceptual framework and apply it to propose a brand management strategy in a specific business scenario. For this purpose, a sectorial cross-validation was developed by triangulating the application of the framework to two data collection methods: (1) interviews and (2) a literature review. The results suggested that decomposing a complex business scenario into single-dimensioned business scenarios can help to propose, enhance, or reframe a brand strategy. The results also suggested that some brand dimensions can be used to lever other brand dimensions, such as brand relationship, which is at the top of CEO/CMO priorities in this field. This work contributes to theory by cross-validating the conceptual framework for creating brand management strategies through triangulation.
Immunobiology and Cytokine Modulation of the Pediatric Brain Tumor Microenvironment: A Scoping Review
Authors: Budhiraja Shreya , Najem Hinda , Tripathi Shashwat , Wadhawani Nitin R. , Horbinski Craig , McCord Matthew , Lenzen Alicia C. , Heimberger Amy B. , DeCuypere Michael
Published: July 2023

Utilizing a Scoping Review strategy in the domain of immune biology to identify immune therapeutic targets, knowledge gaps for implementing immune therapeutic strategies for pediatric brain tumors was assessed. The analysis demonstrated limited efforts to date to characterize and understand the immunological aspects of tumor biology with an over-reliance on observations from the adult glioma population. Foundational knowledge regarding the frequency and ubiquity of immune therapeutic targets is an area of unmet need along with the development of immune-competent pediatric tumor models to test therapeutics and especially combinatorial treatment. Opportunities arise in the evolution of pediatric tumor classification from histological to molecular with targeted immune therapeutics.
Roles of Extracellular Vesicles on the Progression and Metastasis of Hepatocellular Carcinoma
Authors: Seay Turner W. , Suo Zucai
Published: July 2023

Liver cancer is a global health challenge as it is the third leading cause of cancer death worldwide. Hepatocellular carcinoma (HCC) is the most common type of liver cancer and is often found in liver cells, where it is associated with high morbidity and mortality rates. Recent studies have shown that extracellular vesicles (EVs) secreted by HCC cells play a critical role in HCC progression and metastasis. EVs contain proteins, nucleic acids, lipids, and metabolites as cargos. EVs derived from HCC cells can transfer oncogenic factors to surrounding cells leading to increased tumor growth, cell invasion, and angiogenesis. In this review, we summarize the roles that EVs play and the specific effects of their cargos on HCC progression and metastasis and identify potential therapeutic targets for HCC treatment.
Pyrroline-5-Carboxylate Reductase-2 Promotes Colorectal Carcinogenesis by Modulating Microtubule-Associated Serine/Threonine Kinase-like/Wnt/β-Catenin Signaling
Authors: Lama Tamang Raju , Kumar Balawant , Patel Sagar M. , Thapa Ishwor , Ahmad Alshomrani , Kumar Vikas , Ahmad Rizwan , Becker Donald F. , Bastola Dundy (Kiran) , Dhawan Punita , Singh Amar B.
Published: July 2023

Background: Despite significant progress in clinical management, colorectal cancer (CRC) remains the third most common cause of cancer-related deaths. A positive association between PYCR2 (pyrroline-5-carboxylate reductase-2), a terminal enzyme of proline metabolism, and CRC aggressiveness was recently reported. However, how PYCR2 promotes colon carcinogenesis remains ill understood. Methods: A comprehensive analysis was performed using publicly available cancer databases and CRC patient cohorts. Proteomics and biochemical evaluations were performed along with genetic manipulations and in vivo tumor growth assays to gain a mechanistic understanding. Results: PYCR2 expression was significantly upregulated in CRC and associated with poor patient survival, specifically among PYCR isoforms (PYCR1, 2, and 3). The genetic inhibition of PYCR2 inhibited the tumorigenic abilities of CRC cells and in vivo tumor growth. Coinciding with these observations was a significant decrease in cellular proline content. PYCR2 overexpression promoted the tumorigenic abilities of CRC cells. Proteomics (LC-MS/MS) analysis further demonstrated that PYCR2 loss of expression in CRC cells inhibits survival and cell cycle pathways. A subsequent biochemical analysis supported the causal role of PYCR2 in regulating CRC cell survival and the cell cycle, potentially by regulating the expression of MASTL, a cell-cycle-regulating protein upregulated in CRC. Further studies revealed that PYCR2 regulates Wnt/β-catenin-signaling in manners dependent on the expression of MASTL and the cancer stem cell niche. Conclusions: PYCR2 promotes MASTL/Wnt/β-catenin signaling that, in turn, promotes cancer stem cell populations and, thus, colon carcinogenesis. Taken together, our data highlight the significance of PYCR2 as a novel therapeutic target for effectively treating aggressive colon cancer.
The Molecular Pathogenesis of Tumor-Suppressive miR-486-5p and miR-486-3p Target Genes: GINS4 Facilitates Aggressiveness in Lung Adenocarcinoma
Authors: Tomioka Yuya , Suetsugu Takayuki , Seki Naohiko , Tanigawa Kengo , Hagihara Yoko , Shinmura Masahiro , Asai Shunichi , Kikkawa Naoko , Inoue Hiromasa , Mizuno Keiko
Published: July 2023

The involvement of passenger strands of miRNAs in the molecular pathogenesis of human cancers is a recent concept in miRNA research, and it will broaden our understanding of the molecular mechanisms of miRNA-mediated cancer. The analysis of our miRNA signature of LUAD revealed that both strands of pre-miR-486 (miR-486-5p and miR-486-3p) were downregulated in LUAD tissues. Ectopic expression of both miRNAs induced cell cycle arrest in LUAD cells, suggesting both strands of miRNAs derived from pre-miR-486 were tumor suppressive. Our in silico analysis showed a total of 99 genes may be under the control of both miRNAs in LUAD cells. Importantly, among these targets, the high expression of seven genes (MKI67, GINS4, RRM2, HELLS, MELK, TIMELESS, and SAPCD2) predicted a poorer prognosis of LUAD patients (p < 0.05). We focused on GINS4, a DNA replication complex GINS protein that plays an essential role in the initiation of DNA replication. Our functional assays showed that GINS4 was directly controlled by both strands of pre-miR-486, and its aberrant expression facilitated the aggressive behavior of LUAD cells. GINS4 is attractive as a therapeutic target for this disease. MiRNA analysis, including passenger strands, will further improve our understanding of the molecular pathogenesis of LUAD.
Design of a New Type of In-Hole Gold-Coated High-Performance Quasi-PCF Sensor Enhanced with Surface Plasmon Resonance
Authors: Zhou Wenjun , Qin Xi , Lv Ming , Qiu Lifeng , Chen Zhongjiang , Zhang Fan
Published: July 2023

With the development of aerospace, deep-sea exploration and other technologies, the demand for anti-electromagnetic, high-sensitivity and miniaturized sensors is increasingly urgent. In this paper, a model of a quasi-photonic crystal fiber (Q-PCF) refractive index (RI) sensor enhanced with surface plasmon resonance (SPR) is proposed. A stable gold film with a significant SPR effect is applied to the two identically sized and oppositely positioned air holes of the proposed sensor, and all air holes are filled with analyte. A detailed analysis of the mode characteristics, structural parameters and RI sensing performance of the sensor has been carried out using the finite element method. It has been shown that the maximum sensitivity (S) is 4977.59 nm/RIU in the RI range of 1.35–1.40, corresponding to a resolution (R) of 2.01 × 10−5 RIU and a figure of merit (FOM) of 160.36 RIU−1. The proposed Q-PCF sensor has unique fabrication advantages and outstanding sensing properties, providing a new idea for biosensing, complex environment monitoring and long-range measurement, and is of great practical value in the field of highly integrated sensing.
Assesing Climate Change Risk in the Mining Industry: A Case Study in the Copper Industry in the Antofagasta Region, Chile
Authors: Del Rio J. Ignacio , Fernandez Paulina , Castillo Emilio , Orellana Luis Felipe
Published: July 2023

The challenges climate change poses require careful consideration and addressing within specific industries. In the mining context, climate change introduces potential limitations to the sustainable sourcing of minerals, thereby amplifying the criticality of several metals. However, most studies examining mineral criticality fail to assess the localized impacts of climate change, despite significant variations occurring at a relatively small scale. In this study, we propose a methodological approach for conducting a climate risk assessment in the mining industry, encompassing the identification of relevant hazards, vulnerabilities, and exposure specific to the sector. To illustrate the application of this approach, we utilize micro-level data for the Antofagasta region in Chile, a prominent mining cluster situated in a country projected to be profoundly affected by climate change. The findings of this study underscore the necessity for coordinated efforts in adaptation and climate resilience while offering a valuable tool for allocating resources to more vulnerable locations, thus strengthening the mineral supply chain.