bluuebunny/crossref_metadata_2025 huggingface dataset:
Robustness of antiadhesion between nanofibers and surfaces covered with nanoripples of varying spatial period
Authors: Buchberger Gerda , Meyer Marco , Plamadeala Cristina , Weissbach Margret , Hesser Günter , Baumgartner Werner , Heitz Johannes , Joel Anna-Christin
Published: June 2023

Since nanofibers have a high surface-to-volume ratio, van der Waals forces render them attracted to virtually any surface. The high ratio provides significant advantages for applications in drug delivery, wound healing, tissue regeneration, and filtration. Cribellate spiders integrate thousands of nanofibers into their capture threads as an adhesive to immobilize their prey. These spiders have antiadhesive nanoripples on the calamistrum, a comb-like structure on their hindmost legs, and are thus an ideal model for investigating how nanofiber adhesion can be reduced. We found that these nanoripples had similar spacing in the cribellate species Uloborus plumipes, Amaurobius similis, and Menneus superciliosus, independent of phylogenetic relation and size. Ripple spacing on other body parts (i.e., cuticle, claws, and spinnerets), however, was less homogeneous. To investigate whether a specific distance between the ripples determines antiadhesion, we fabricated nanorippled foils by nanosecond UV laser processing. We varied the spatial periods of the nanoripples in the range ~ 203–613 nm. Using two different pulse numbers resulted in ripples of different heights. The antiadhesion was measured for all surfaces, showing that the effect is robust against alterations across the whole range of spatial periods tested. Motivated by these results, we fabricated irregular surface nanoripples with spacing in the range ~ 130–480 nm, which showed the same antiadhesive behavior. The tested surfaces may be useful in tools for handling nanofibers such as spoolers for single nanofibers, conveyor belts for producing endless nanofiber nonwoven, and cylindrical tools for fabricating tubular nanofiber nonwoven. Engineered fibers such as carbon nanotubes represent a further candidate application area.
The exposure to volatile organic chemicals associates positively with rheumatoid arthritis: a cross-sectional study from the NHANES program
Authors: Lei Ting , Qian Hu , Yang Junxiao , Hu Yihe
Published: June 2023

Introduction

Rheumatoid arthritis (RA) is an autoimmune disease and closely associated with both genetic and environmental factors. Volatile organic chemicals (VOC), a common environment pollutant, was associated with some autoimmune diseases, while whether VOC exposure or which VOC leads to RA is yet clarified.

Methods

A cross-sectional study using data from the 6 survey cycles (2005-2006, 2011-2012, 2013-2014, 2015-2016, 2017-2018, 2017-2020) of NHANES program was performed. The RA or non-arthritis status of participant was identified through a questionnaire survey. The quantile logistic regression method was used for correlation analysis between VOC metabolites (VOCs) in urine and RA. The covariates included age, gender, race, educational level, marital status, total energy intake, physical activity, smoking, hypertension, diabetes, urine creatinine, albumin and marihuana use.

Results

A total of 9536 participants (aged 20 to 85) with 15 VOCs, comprising 618 RA and 8918 non-arthritis participants, was finally included for analysis. Participants in the RA group showed higher VOCs in urine than that in the non-arthritis group. A positive association between 2 VOCs (AMCC: Q4: OR=2.173, 95%CI: 1.021, 4.627. 3HPMA: Q2: OR=2.286, 95%CI: 1.207 - 4.330; Q4: OR=2.663, 95%CI: 1.288 -5.508.) and RA was detected in the model 3, which was independent of all the covariates. The relative parent compounds of the two VOCs included N,N-Dimethylformamide and acrolein.

Discussion

These findings suggested that the VOC exposure significantly associated with RA, providing newly epidemiological evidence for the establishment that environmental pollutants associated with RA. And also, more prospective studies and related experimental studies are needed to further validate the conclusions of this study.
Transcriptome sequencing and single-cell sequencing analysis identify GARS1 as a potential prognostic and immunotherapeutic biomarker for multiple cancers, including bladder cancer
Authors: Nie Jianqiang , Liu Taobin , Mao Taotao , Yang Hailang , Deng Wen , Liu Xiaoqiang , Fu Bin
Published: June 2023

Background

Glycyl-tRNA synthetase 1 (GARS1) belongs to the aminoacyl-tRNA synthetase family, playing a crucial role in protein synthesis. Previous studies have reported a close association between GARS1 and various tumors. However, the role of GARS1 in human cancer prognosis and its impact on immunology remain largely unexplored.

Methods

In this study, we comprehensively analyzed GARS1 expression at the mRNA and protein levels, examined genetic alterations, and assessed its prognostic implications in pan-cancer, with a specific emphasis on the immune landscape. Furthermore, we investigated the functional enrichment of genes related to GARS1 and explored its biological functions using single-cell data. Finally, we conducted cellular experiments to validate the biological significance of GARS1 in bladder cancer cells.

Results

In general, GARS1 expression was significantly upregulated across multiple cancer types, and it demonstrated prognostic value in various cancers. Gene Set Enrichment Analysis (GSEA) revealed the association of GARS1 expression with multiple immune regulatory pathways. Moreover, GARS1 exhibited significant correlations with immune infiltrating cells (such as DC, CD8+T cells, Neutrophils, and Macrophages), immune checkpoint genes (CD274, CD276), and immune regulatory factors in tumors. Additionally, we observed that GARS1 could effectively predict the response to anti-PD-L1 therapy. Notably, Ifosfamide, auranofin, DMAPT, and A-1331852 emerged as potential therapeutic agents for GARS1-upregulated tumors. Our experimental findings strongly suggest that GARS1 promotes the proliferation and migration of bladder cancer cells.

Conclusion

GARS1 holds promise as a potential prognostic marker and therapeutic target for pan-cancer immunotherapy, offering valuable insights for the development of more precise and personalized approaches to tumor treatment in the future.
Paxbp1 is indispensable for the survival of CD4 and CD8 double-positive thymocytes
Authors: Li Wenting , Yang Yang , Liu Shenglin , Zhang Dongsheng , Ren Xuanyao , Tang Mindan , Zhang Wei , Chen Xiaofan , Huang Cong , Yu Bo
Published: June 2023

The lifespan of double-positive (DP) thymocytes is critical for intrathymic development and shaping the peripheral T cell repertoire. However, the molecular mechanisms that control DP thymocyte survival remain poorly understood. Paxbp1 is a conserved nuclear protein that has been reported to play important roles in cell growth and development. Its high expression in T cells suggests a possible role in T cell development. Here, we observed that deletion of Paxbp1 resulted in thymic atrophy in mice lacking Paxbp1 in the early stages of T cell development. Conditional loss of Paxbp1 resulted in fewer CD4+CD8+ DP T cells, CD4 and CD8 single positive (SP) T cells in the thymus, and fewer T cells in the periphery. Meanwhile, Paxbp1 deficiency had limited effects on the CD4-CD8- double negative (DN) or immature single-positive (ISP) cell populations. Instead, we observed a significant increase in the susceptibility of Paxbp1-deficient DP thymocytes to apoptosis. Consistent with this, RNA-Seq analysis revealed a significant enrichment of the apoptotic pathway within differentially expressed genes in Paxbp1-deficient DP cells compared to control DP cells. Together, our results suggest a new function for Paxbp1, which is an important mediator of DP thymocyte survival and critical for proper thymic development.
Single cell transcriptome profiling reveals cutaneous immune microenvironment remodeling by photodynamic therapy in photoaged skin
Authors: Yan Yu , Yan Guorong , Cao Zhi , Wang Bo , Zeng Qingyu , Shi Lei , Chang Qihang , Chen Chengqian , Zhang Linglin , Liao Caihe , Jin Shengkai , Sun Xiaofei , Zhang Guolong , Wang Peiru , Wang Xiuli
Published: June 2023

Background

The immune microenvironment plays a critical role in maintaining skin homeostasis, which is closely related to the dysfunction in photoaged skin such as autoimmunity and tumorigenesis. Several recent studies have demonstrated the efficacy of 5-aminolevulinic acid photodynamic therapy (ALA-PDT) in alleviating photoaging and skin cancer. However, the underlying immune mechanisms and the immune microenvironment change by ALA-PDT remain largely unknown.

Methods

To illustrate the effects of ALA-PDT on immune microenvironment in photoaged skin, single cell RNA sequencing (scRNA-seq) analysis of photoaged skin on the extensor side of the human forearm before and after ALA-PDT was performed. R-packages of Seurat, clusterProfiler, Monocle, CellChat were used for cell clustering, differentially expressed genes analysis, functional annotation, pseudotime analysis and cell-cell communication analysis. The gene sets related to specific functions were extracted from the MSigDB database, which were used to score the functions of immune cells in different states. We also compared our result with published scRNA-seq data of photoaged skin of the eyelids.

Results

The increase score of cellular senescence, hypoxia and reactive oxygen species pathway in immune cells and the decrease of immune receptor activity function and proportion of naive T cells were found in skin photoaging. Moreover, the function of T cell ribosomal synthesis was also impaired or down regulated and function of G2M checkpoint was up regulated. However, ALA-PDT showed promising results in reversing these effects, as it improved the above functions of T cells. The ratio of M1/M2 and percentage of Langerhans cells also decreased with photoaging and increased after ALA-PDT. Additionally, ALA-PDT restored the antigen presentation and migration function of dendritic cells and enhanced cell-cell communication among immune cells. These effects were observed to last for 6 months.

Conclusion

ALA-PDT has potential to rejuvenate immune cells, partially reversed immunosenescence and improved the immunosuppressive state, ultimately remodelling the immune microenvironment in photoaged skin. These results provide an important immunological basis for further exploring strategies to reverse skin photoaging, chronological aging and potentially systemic aging.
Case Report: Chronic hepatitis E virus Infection in an individual without evidence for immune deficiency
Authors: Ying Dong , Niu Wenxia , Chen Yanling , Wang Yingbin , Tian Weikun , Zhang Xiaoping , Liu Chang , Wang Siling , Chen Zihao , Lin Yajie , Guo Shaoqi , Yu Zihao , Chen Xiuting , Fang Mujin , Qiang Hongsheng , Yin Yifan , Tang Zimin , Zheng Zizheng , Fu Lijuan , Xia Ningshao
Published: June 2023

Chronic hepatitis E virus (HEV) infection occurs mainly in immunosuppressed populations. We describe an investigation of chronic HEV infection of genotype 3a in an individual without evidence for immune deficiency who presented hepatitis with significant HEV viremia and viral shedding. We monitored HEV RNA in plasma and stools, and assessed anti-HEV specific immune responses. The patient was without apparent immunodeficiency based on quantified results of white blood cell, lymphocyte, neutrophilic granulocyte, CD3+ T cell, CD4+ T cell, and CD8+ T cell counts and CD4/CD8 ratio, as well as total serum IgG, IgM, and IgA, which were in the normal range. Despite HEV specific cellular response and strong humoral immunity being observed, viral shedding persisted up to 109 IU/mL. After treatment with ribavirin combined with interferon, the indicators of liver function in the patient returned to normal, accompanied by complete suppression and clearance of HEV. These results indicate that HEV chronicity can also occur in individuals without evidence of immunodeficiency.
RETRACTED: Junior secondary school students' motivation for learning multiple foreign languages in Burundi: a cross-sectional study
Authors: Nizigama Elvis , Fazilatfar Ali Mohammad , Jabbari Ali Akbar
Published: June 2023

This study investigated the motivation of Burundi junior secondary school students learning simultaneously three foreign languages: French, English, and Kiswahili. A 65-item questionnaire was employed to sample the views of 348 (grade 7 through 9) learners from state schools. Using L2 motivational self system developed by Dörnyei as the main theoretical framework, this study specifically explored temporal changes in L2 motivation of the participants over the 3-year period across the three target languages. The results indicated that there were significant differences in most of the measured motivational scales across the three languages with ninth grade students scoring significantly lower than their peers from lower school grades. Moreover, the results revealed that L2 learning experience and ideal L2 self were the two variables that, respectively, predicted most the students' intended effort to learn each language. One school grade-related difference was that international posture appeared as a significant factor among seventh grade students in relation to French learning and among ninth grade pupils regarding English learning. Another finding, which was unexpected, was that English (L3) appeared to be the most popular foreign language among these students. In the light of the results, pedagogical implications are discussed based on the socio-educational context of Burundi.
Coral reef island shoreline change and the dynamic response of the freshwater lens, Huvadhoo Atoll, Maldives
Authors: Carruthers Lucy , East Holly , Ersek Vasile , Suggitt Andrew , Campbell Millie , Lee Kara , Naylor Victoria , Scurrah Dominic , Taylor Liam
Published: June 2023

Low-lying coral reef islands have been projected to become uninhabitable by the end of the century due to sea level rise, but such projections of vulnerability assume that reef islands are static landforms that flood incrementally with sea level rise. In fact, GIS-based reef island shoreline analyses have demonstrated that reef islands are highly dynamic landforms that may adjust their shorelines in response to changing environmental conditions. However, the vast majority of reef island shoreline analyses have been undertaken in the Pacific Ocean, leaving our understanding of changes in the Indian Ocean more limited. Further, our knowledge of how island dynamics can impact groundwater resources is restricted due to the assumption that islands will exhibit purely erosional responses to sea level rise. Here, we analyse shoreline evolution on 49 reef islands over a 50-year timeframe in Huvadhoo Atoll, Maldives. Additionally, rates of shoreline change were used to undertake numerical modelling of shifts in freshwater lens volume in 2030, 2050 and 2100 in response to changes in recharge. Despite sea level rising at 4.24 mm/year (1969-2019), accretion was prevalent on 53% of islands, with the remaining islands eroding (25%) or remaining stable (22%). Average net shoreline movement was 4.13 m, ranging from -17.51 to 65.73 m; and the average rate of shoreline change (weighted linear regression) was 0.13 m/year, ranging from -0.07 to 2.65 m/year. The magnitudes and rates of reef island evolution were found to be highly site-specific, with island type found to be the only significant predictor of either net shoreline movement or weighted linear regression. Results suggest that freshwater lens volume was substantially impacted by shoreline change compared to changes in recharge whereby accretion and erosion led to large increases (up to 65.05%) decreases (up to -50.4%) in les volume, respectively. We suggest that the capacity of reef islands to both (1) adjust their shorelines, and even accrete, under conditions of sea level rise; and (2) increase their storage of groundwater over the coming decades represents highly valuable geomorphic ecosystem services.
Modelling the biogeochemical footprint of rivers in the Hauraki Gulf, New Zealand
Authors: Macdonald Helen , Collins Charine , Plew David , Zeldis John , Broekhuizen Niall
Published: June 2023

Building accurate physical-biogeochemical models of processes driving climate and eutrophication-related stressors in coastal waters is an essential step in managing the impacts of these stressors. Here we develop a coupled physical-biogeochemical model to investigate present day processes for a key marine ecosystem in Aotearoa, New Zealand: The Hauraki Gulf/Firth of Thames system. Simulation results compared well with an accompanying long-term (decadal) observational dataset, indicating that the model captured most of the physical and biological dynamics of the Hauraki Gulf/Firth of Thames system. This model was used to investigate the riverine and cross shelf exchanges of nutrients in the region and showed that only a small number of large rivers within the Firth of Thames dominated the freshwater inputs, with phytoplankton concentrations driven by nutrient inputs from these rivers. However, while riverine inputs dominated the biological response in the Firth of Thames, cross-shelf fluxes dominated the biological response in the outer Hauraki Gulf region. Nutrients from both sources were balanced by a sediment denitrification flux. Analyses were conducted to examine agreement of observations with subsampled and climatological model outputs. These revealed that modelling effort needs to focus on the representation of sediment fluxes and parameterizations during the autumn, and the observational effort needs to focus on increased temporal data collection during summer to better understand biases in seasonal climatologies derived from model and observations. These results are valuable for demonstrating effects of land-derived and oceanic drivers of the biogeochemical dynamics of the Hauraki Gulf/Firth of Thames system.
Investigation of carboxymethyl chitosan in the development of biodegradable soft bait fishing lures
Authors: Legault Ryan , Ahmadi Ali
Published: June 2023

This paper describes the development and testing of two compositions of biodegradable soft bait fishing lures. A water-soluble form of chitosan, known as carboxymethyl chitosan, was added to the biodegradable lure formula to investigate if the lure properties, such as tensile strength, swelling, and underwater performance, could be improved. A three-month shelf-life study was completed to compare the lure properties of two compositions of biodegradable baits: the first composition containing no carboxymethyl chitosan and the second composition containing 5 wt.% carboxymethyl chitosan. The baits manufactured with carboxymethyl chitosan showed increased tensile strength and underwater performance compared to the lures manufactured without this ingredient. Also, the lures manufactured with carboxymethyl chitosan showed increased swelling when submerged in fresh water, which is not desired; however, these lures stayed intact longer before beginning to degrade. When submerged in salt water, the lures manufactured with and without carboxymethyl chitosan showed similar characteristics. The following results will assist in completing further formula optimizations to improve other hindering properties of the current biodegradable lures. The development of more environmentally friendly fishing options is needed to preserve the world’s oceans and freshwater systems for the future generation of recreational anglers.
Flood-driven jet flow and sedimentary regime in a river-dominated estuary
Authors: Liu Jing , Lin Yitong , He Zixiao , Liu Feng , Jia Liangwen , Wei Wen
Published: June 2023

Sediment dynamics under floods are critical for estuarine morphological evolution. A two-dimensional coupled hydrodynamic/sediment transport numerical modeling was adapted to investigate the flood-driven jet structure and the resultant sedimentary regime in a river-dominated estuary, Modaomen Estuary, Pearl River Delta, China. The results show that the flow velocity of flood jet exhibits a Gaussian distribution in the transverse direction and shows a linear decreasing trend in the longitudinal direction. Moreover, a distinct zonation, including a zone of flow establishment (ZOFE) and a zone of established flow (ZOEF), was detected. The ZOFE was the core area of high turbidity and strong erosion and provided sufficient sediments to the ZOEF, where sediment diffused and settled. Due to the settling lag, the sediment diffusion and deposition areas were larger than those of the jet. Estuarine geomorphology regulates the shape of flood jet, which shows an asymmetry configuration with the main body located at the west side of the bifurcated estuary. Tidal dynamics play a crucial role in altering the range of jet diffusion, especially in the longitudinal direction. Increased flood intensity has little effect on the jet structure and erosion/deposition pattern, but significantly increases the magnitude of seaward current velocities and bed level changes, as well as the transverse diffusion range of jet and sedimentation.
Identification and validation of aging-related gene signatures and their immune landscape in diabetic nephropathy
Authors: Liang Yingchao , Liang Zhiyi , Huang Jinxian , Jia Mingjie , Liu Deliang , Zhang Pengxiang , Fang Zebin , Hu Xinyu , Li Huilin
Published: June 2023

Background

Aging and immune infiltration have essential role in the physiopathological mechanisms of diabetic nephropathy (DN), but their relationship has not been systematically elucidated. We identified aging-related characteristic genes in DN and explored their immune landscape.

Methods

Four datasets from the Gene Expression Omnibus (GEO) database were screened for exploration and validation. Functional and pathway analysis was performed using Gene Set Enrichment Analysis (GSEA). Characteristic genes were obtained using a combination of Random Forest (RF) and Support Vector Machine Recursive Feature Elimination (SVM-RFE) algorithm. We evaluated and validated the diagnostic performance of the characteristic genes using receiver operating characteristic (ROC) curve, and the expression pattern of the characteristic genes was evaluated and validated. Single-Sample Gene Set Enrichment Analysis (ssGSEA) was adopted to assess immune cell infiltration in samples. Based on the TarBase database and the JASPAR repository, potential microRNAs and transcription factors were predicted to further elucidate the molecular regulatory mechanisms of the characteristic genes.

Results

A total of 14 differentially expressed genes related to aging were obtained, of which 10 were up-regulated and 4 were down-regulated. Models were constructed by the RF and SVM-RFE algorithms, contracted to three signature genes: EGF-containing fibulin-like extracellular matrix (EFEMP1), Growth hormone receptor (GHR), and Vascular endothelial growth factor A (VEGFA). The three genes showed good efficacy in three tested cohorts and consistent expression patterns in the glomerular test cohorts. Most immune cells were more infiltrated in the DN samples compared to the controls, and there was a negative correlation between the characteristic genes and most immune cell infiltration. 24 microRNAs were involved in the transcriptional regulation of multiple genes simultaneously, and Endothelial transcription factor GATA-2 (GATA2) had a potential regulatory effect on both GHR and VEGFA.

Conclusion

We identified a novel aging-related signature allowing assessment of diagnosis for DN patients, and further can be used to predict immune infiltration sensitivity.
Intermittent parathyroid hormone enhances the healing of medication-related osteonecrosis of the jaw lesions in rice rats
Authors: Castillo E. J. , Jiron J. M. , Croft C. S. , Freehill D. G. , Castillo C. M. , Kura J. , Yarrow J. F. , Bhattacharyya I. , Kimmel D. B. , Aguirre J. Ignacio
Published: June 2023

Medication-related osteonecrosis of the jaw (MRONJ) is a potentially severe adverse event in patients treated with antiresorptives. Management of MRONJ is challenging, and no non-antibiotic, established medical treatment exists. Intermittent parathyroid hormone (iPTH) has been used off-label to treat MRONJ with favorable results. However, its medical efficacy has rarely been substantiated in clinical or preclinical experiments. Using a validated rice rat, infection-based model of MRONJ, we evaluated the effects of iPTH on established MRONJ. We hypothesize that iPTH contributes to MRONJ resolution by enhancing alveolar bone turnover and healing oral soft tissues. Eighty-four rice rats began a standard rodent chow diet at age 4 weeks to induce localized periodontitis. Rats were simultaneously randomized to receive saline (vehicle, VEH) or zoledronic acid (ZOL, 80 μg/kg IV) every 4 weeks. Oral exams were conducted bi-weekly to assign a gross quadrant grade (GQG, 0–4) to evaluate any lesion at the lingual aspect of the interdental space between maxillary molar (M2) and M3. 14 of 20 VEH-treated rice rats (70%) developed maxillary localized periodontitis with GQG 2–3 after 30 ± 10 weeks of saline. Additionally, 40 of 64 ZOL-treated rice rats with periodontitis developed MRONJ-like lesions after 30 ± 10 weeks of ZOL treatment. Rice rats with localized periodontitis or MRONJ-like lesions were treated with saline or iPTH (40 μg/kg) subcutaneously (SC) 3 times/week For 6 weeks until euthanasia. We found that iPTH -treated ZOL rats had a lower prevalence of MRONJ (p < 0.001), with lower severity extent of oral lesions (p = 0.003) and percentage of empty osteocyte lacunae (p < 0.001). ZOL rats treated with iPTH displayed a higher osteoblast surface (p < 0.001), more osteoblasts (p < 0.001), higher osteoclast surface (p < 0.001) and more osteoclasts (p = 0.002) at alveolar bone surfaces than ZOL/VEH rats. Greater gingival epithelial thickness and epithelial cell proliferation rate was found in the oral mucosa and gingiva of ZOL/PTH rats than in ZOL/VEH rats (p < 0.001). Our data suggest that iPTH is an efficacious non-operative medicinal therapy that accelerates oral healing and enhances the resolution of MRONJ lesions in ZOL-treated rice rats.
Oxygen and mechanical stretch in the developing lung: risk factors for neonatal and pediatric lung disease
Authors: Zhang Emily Y. , Bartman Colleen M. , Prakash Y. S. , Pabelick Christina M. , Vogel Elizabeth R.
Published: June 2023

Chronic airway diseases, such as wheezing and asthma, remain significant sources of morbidity and mortality in the pediatric population. This is especially true for preterm infants who are impacted both by immature pulmonary development as well as disproportionate exposure to perinatal insults that may increase the risk of developing airway disease. Chronic pediatric airway disease is characterized by alterations in airway structure (remodeling) and function (increased airway hyperresponsiveness), similar to adult asthma. One of the most common perinatal risk factors for development of airway disease is respiratory support in the form of supplemental oxygen, mechanical ventilation, and/or CPAP. While clinical practice currently seeks to minimize oxygen exposure to decrease the risk of bronchopulmonary dysplasia (BPD), there is mounting evidence that lower levels of oxygen may carry risk for development of chronic airway, rather than alveolar disease. In addition, stretch exposure due to mechanical ventilation or CPAP may also play a role in development of chronic airway disease. Here, we summarize the current knowledge of the impact of perinatal oxygen and mechanical respiratory support on the development of chronic pediatric lung disease, with particular focus on pediatric airway disease. We further highlight mechanisms that could be explored as potential targets for novel therapies in the pediatric population.
Study on diversity, nitrogen-fixing capacity, and heavy metal tolerance of culturable Pongamia pinnata rhizobia in the vanadium-titanium magnetite tailings
Authors: Shen Tian , Jin Ruimin , Yan Jing , Cheng Xiran , Zeng Lan , Chen Qiang , Gu Yunfu , Zou Likou , Zhao Ke , Xiang Quanju , Penttinen Petri , Ma Menggen , Li Shuangcheng , Zou Ting , Yu Xiumei
Published: June 2023

Introduction

The diversity, nitrogen-fixing capacity and heavy metal tolerance of culturable rhizobia in symbiotic relationship with Pongamia pinnata surviving in vanadium (V) - titanium (Ti) magnetite (VTM) tailings is still unknown, and the rhizobia isolates from the extreme barren VTM tailings contaminated with a variety of metals would provide available rhizobia resources for bioremediation.

Methods

P. pinnata plants were cultivated in pots containing the VTM tailings until root nodules formed, and then culturable rhizobia were isolated from root nodules. The diversity, nitrogen-fixing capacity and heavy metal tolerance of rhizobia were performed.

Results

Among 57 rhizobia isolated from these nodules, only twenty strains showed different levels of tolerance to copper (Cu), nickel (Ni), manganese (Mn) and zinc (Zn), especially strains PP1 and PP76 showing high tolerance against these four heavy metals. Based on the phylogenetic analysis of 16S rRNA and four house-keeping genes (atpD, recA, rpoB, glnII), twelve isolates were identified as Bradyrhizobium pachyrhizi, four as Ochrobactrum anthropic, three as Rhizobium selenitireducens and one as Rhizobium pisi. Some rhizobia isolates showed a high nitrogen-fixing capacity and promoted P. pinnata growth by increasing nitrogen content by 10%-145% in aboveground plant part and 13%-79% in the root. R. pachyrhizi PP1 showed the strongest capacity of nitrogen fixation, plant growth promotion and resistance to heavy metals, which provided effective rhizobia strains for bioremediation of VTM tailings or other contaminated soils. This study demonstrated that there are at least three genera of culturable rhizobia in symbiosis with P. pinnata in VTM tailings.

Discussion

Abundant culturable rhizobia with the capacity of nitrogen fixation, plant growth promotion and resistance to heavy metals survived in VTM tailings, indicating more valuable functional microbes could be isolated from extreme soil environments such as VTM tailings.
Porphyromonas gingivalis induces an inflammatory response via the cGAS-STING signaling pathway in a periodontitis mouse model
Authors: Bi Rong , Yang Yanling , Liao Hongwei , Ji Guang , Ma Yan , Cai Lukui , Li Jingyan , Yang Jingsi , Sun Mingbo , Liang Jiangli , Shi Li
Published: June 2023

Periodontitis is an inflammatory disease initiated by periodontopathogenic bacteria in the dental plaque biofilms. Understanding the role of Porphyromonas gingivalis (P. gingivalis), a keystone pathogen associated with chronic periodontitis, in the inflammatory response is crucial. Herein, we investigated whether P. gingivalis infection triggers the expression of the type I IFN gene and various cytokines and leads to activation of the cGAMP synthase–stimulator of IFN genes (cGAS-STING) pathway both in vitro and in a mouse model. Additionally, in an experimental model of periodontitis using P. gingivalis, StingGt mice showed lower levels of inflammatory cytokines and bone resorption than wild-type mice. Furthermore, we report that a STING inhibitor (SN-011) significantly decreased inflammatory cytokine production and osteoclast formation in a periodontitis mouse model with P. gingivalis. In addition, STING agonist (SR-717) -treated periodontitis mice displayed enhanced macrophage infiltration and M1 macrophage polarization in periodontal lesions compared with that in vehicle-treated periodontitis mice. In conclusion, our results demonstrate that the cGAS-STING signaling pathway may be one of the key mechanisms crucial for the P. gingivalis-induced inflammatory response that leads to chronic periodontitis.
Gut microbiota and chronic obstructive pulmonary disease: a Mendelian randomization study
Authors: Wei Yi , Lu Xuechao , Liu Chao
Published: June 2023

Background

A growing number of studies implies a strong association between gut microbiota and chronic obstructive pulmonary disease (COPD). However, the causal impact between gut microbiota and COPD remains unclear. As a result, we used a two-sample Mendelian randomization (MR) method to investigate the connection between gut microbiota and COPD in this study.

Methods

The largest available genome-wide association study (GWAS) of gut microbiota was obtained from the MiBioGen consortium. Summary-level dataset for COPD were obtained from the FinnGen consortium. The main analysis method for determining the causal link between gut microbiota and COPD was inverse variance weighted (IVW). Subsequently, pleiotropy and heterogeneity tests were performed to determine the reliability of the results.

Results

IVW method identified 9 bacterial taxa nominally associated with the risk of COPD. Class Actinobacteria (p = 0.020), genus Allisonella (p = 0.024), genus Coprococcus2 (p = 0.002) and genus Oscillospira (p = 0.018) were protective against COPD. In addition, order Desulfovibrionales (p = 0.011), family Desulfovibrionaceae (p = 0.039), family Peptococcaceae (p = 0.020), family Victivallaceae (p = 0.012) and genus Marvinbryantia (p = 0.017) were associated with a higher risk of COPD. No pleiotropy or heterogeneity were found.

Conclusion

According to the findings of this MR analysis, a causal relationship exists between certain gut microbiota and COPD. New insights into the mechanisms of COPD mediated by gut microbiota are provided.
Understanding health behavior change by motivation and reward mechanisms: a review of the literature
Authors: Michaelsen Maren M. , Esch Tobias
Published: June 2023

The global rise of lifestyle-related chronic diseases has engendered growing interest among various stakeholders including policymakers, scientists, healthcare professionals, and patients, regarding the effective management of health behavior change and the development of interventions that facilitate lifestyle modification. Consequently, a plethora of health behavior change theories has been developed with the intention of elucidating the mechanisms underlying health behavior change and identifying key domains that enhance the likelihood of successful outcomes. Until now, only few studies have taken into account neurobiological correlates underlying health behavior change processes. Recent progress in the neuroscience of motivation and reward systems has provided further insights into the relevance of such domains. The aim of this contribution is to review the latest explanations of health behavior change initiation and maintenance based on novel insights into motivation and reward mechanisms. Based on a systematic literature search in PubMed, PsycInfo, and Google Scholar, four articles were reviewed. As a result, a description of motivation and reward systems (approach/wanting = pleasure; aversion/avoiding = relief; assertion/non-wanting = quiescence) and their role in health behavior change processes is presented. Three central findings are discussed: (1) motivation and reward processes allow to distinguish between goal-oriented and stimulus-driven behavior, (2) approach motivation is the key driver of the individual process of behavior change until a new behavior is maintained and assertion motivation takes over, (3) behavior change techniques can be clustered based on motivation and reward processes according to their functional mechanisms into facilitating (= providing external resources), boosting (= strengthening internal reflective resources) and nudging (= activating internal affective resources). The strengths and limitations of these advances for intervention planning are highlighted and an agenda for testing the models as well as future research is proposed.
Evaluation of fMRI activation in post-stroke patients with movement disorders after repetitive transcranial magnetic stimulation: a scoping review
Authors: Cheng Siman , Xin Rong , Zhao Yan , Wang Pu , Feng Wuwei , Liu Peng
Published: June 2023

Background

Movement disorders are one of the most common stroke residual effects, which cause a major stress on their families and society. Repetitive transcranial magnetic stimulation (rTMS) could change neuroplasticity, which has been suggested as an alternative rehabilitative treatment for enhancing stroke recovery. Functional magnetic resonance imaging (fMRI) is a promising tool to explore neural mechanisms underlying rTMS intervention.

Object

Our primary goal is to better understand the neuroplastic mechanisms of rTMS in stroke rehabilitation, this paper provides a scoping review of recent studies, which investigate the alteration of brain activity using fMRI after the application of rTMS over the primary motor area (M1) in movement disorders patients after stroke.

Method

The database PubMed, Embase, Web of Science, WanFang Chinese database, ZhiWang Chinese database from establishment of each database until December 2022 were included. Two researchers reviewed the study, collected the information and the relevant characteristic extracted to a summary table. Two researchers also assessed the quality of literature with the Downs and Black criteria. When the two researchers unable to reach an agreement, a third researcher would have been consulted.

Results

Seven hundred and eleven studies in all were discovered in the databases, and nine were finally enrolled. They were of good quality or fair quality. The literature mainly involved the therapeutic effect and imaging mechanisms of rTMS on improving movement disorders after stroke. In all of them, there was improvement of the motor function post-rTMS treatment. Both high-frequency rTMS (HF-rTMS) and low-frequency rTMS (LF-rTMS) can induce increased functional connectivity, which may not directly correspond to the impact of rTMS on the activation of the stimulated brain areas. Comparing real rTMS with sham group, the neuroplastic effect of real rTMS can lead to better functional connectivity in the brain network in assisting stroke recovery.

Conclusion

rTMS allows the excitation and synchronization of neural activity, promotes the reorganization of brain function, and achieves the motor function recovery. fMRI can observe the influence of rTMS on brain networks and reveal the neuroplasticity mechanism of post-stroke rehabilitation. The scoping review helps us to put forward a series of recommendations that might guide future researchers exploring the effect of motor stroke treatments on brain connectivity.
Enhancing collaborative neuroimaging research: introducing COINSTAC Vaults for federated analysis and reproducibility
Authors: Martin Dylan , Basodi Sunitha , Panta Sandeep , Rootes-Murdy Kelly , Prae Paul , Sarwate Anand D. , Kelly Ross , Romero Javier , Baker Bradley T. , Gazula Harshvardhan , Bockholt Jeremy , Turner Jessica A. , Esper Nathalia B. , Franco Alexandre R. , Plis Sergey , Calhoun Vince D.
Published: June 2023

Collaborative neuroimaging research is often hindered by technological, policy, administrative, and methodological barriers, despite the abundance of available data. COINSTAC (The Collaborative Informatics and Neuroimaging Suite Toolkit for Anonymous Computation) is a platform that successfully tackles these challenges through federated analysis, allowing researchers to analyze datasets without publicly sharing their data. This paper presents a significant enhancement to the COINSTAC platform: COINSTAC Vaults (CVs). CVs are designed to further reduce barriers by hosting standardized, persistent, and highly-available datasets, while seamlessly integrating with COINSTAC's federated analysis capabilities. CVs offer a user-friendly interface for self-service analysis, streamlining collaboration, and eliminating the need for manual coordination with data owners. Importantly, CVs can also be used in conjunction with open data as well, by simply creating a CV hosting the open data one would like to include in the analysis, thus filling an important gap in the data sharing ecosystem. We demonstrate the impact of CVs through several functional and structural neuroimaging studies utilizing federated analysis showcasing their potential to improve the reproducibility of research and increase sample sizes in neuroimaging studies.
Insulin-like growth factor 1 supplementation supports motor coordination and affects myelination in preterm pigs
Authors: Christiansen Line I. , Ventura Gemma C. , Holmqvist Bo , Aasmul-Olsen Karoline , Lindholm Sandy E. H. , Lycas Matthew D. , Mori Yuki , Secher Jan Bojsen-Møller , Burrin Douglas G. , Thymann Thomas , Sangild Per T. , Pankratova Stanislava
Published: June 2023

Introduction

Preterm infants have increased risk of impaired neurodevelopment to which reduced systemic levels of insulin-like growth factor 1 (IGF-1) in the weeks after birth may play a role. Hence, we hypothesized that postnatal IGF-1 supplementation would improve brain development in preterm pigs, used as a model for preterm infants.

Methods

Preterm pigs delivered by cesarean section received recombinant human IGF-1/IGF binding protein-3 complex (rhIGF-1/rhIGFBP-3, 2.25 mg/kg/day) or vehicle from birth to postnatal day 19. Motor function and cognition were assessed by monitoring of in-cage and open field activities, balance beam test, gait parameters, novel object recognition and operant conditioning tests. Collected brains were subject to magnetic resonance imaging (MRI), immunohistochemistry, gene expression analyses and protein synthesis measurements.

Results

The IGF-1 treatment increased cerebellar protein synthesis rates (both in vivo and ex vivo). Performance in the balance beam test was improved by IGF-1 but not in other neurofunctional tests. The treatment decreased total and relative caudate nucleus weights, without any effects to total brain weight or grey/white matter volumes. Supplementation with IGF-1 reduced myelination in caudate nucleus, cerebellum, and white matter regions and decreased hilar synapse formation, without effects to oligodendrocyte maturation or neuron differentiation. Gene expression analyses indicated enhanced maturation of the GABAergic system in the caudate nucleus (decreased NKCC1:KCC2 ratio) with limited effects in cerebellum or hippocampus.

Conclusion

Supplemental IGF-1 during the first three weeks after preterm birth may support motor function by enhancing GABAergic maturation in the caudate nucleus, despite reduced myelination. Supplemental IGF-1 may support postnatal brain development in preterm infants, but more studies are required to identify optimal treatment regimens for subgroups of very or extremely preterm infants.
High unhealthy food and beverage consumption is associated with poor diet quality among 12–35-month-olds in Guédiawaye Department, Senegal
Authors: Vanderkooy Anna , Ferguson Elaine L. , Sy Ndèye Yaga , Kane Rosenette , Diagne Maty , Mbodji Aminata , Pries Alissa M.
Published: June 2023

Background

High consumption of unhealthy foods and beverages (UFB) during early childhood is cause for concern, with growing evidence from low- and middle-income countries finding associations with poor diet quality and malnutrition. Research from sub-Saharan Africa remains limited, with no studies quantifying the contribution of UFB to total energy intakes among young children or exploring the relationship between such intakes and diet quality or anthropometric outcomes.

Objectives

Assess UFB consumption patterns and their contribution to total energy intake from non-breastmilk foods/beverages (TEI-NBF), assess the association between high UFB consumption and dietary/nutrition outcomes, and explore drivers of unhealthy food choice among young children in Guédiawaye Department, Senegal.

Methods

We conducted a cross-sectional study of a representative sample of 724 primary caregivers and their 12–35.9-month-old children. The study included a questionnaire, a quantitative four-pass 24-h dietary recall, and anthropometric measurements. The contribution of UFB to TEI-NBF was calculated and terciles generated. Logistic and linear models were used to compare outcomes of high versus low UFB consumption terciles.

Results

UFB contributed on average 22.2% of TEI-NBF, averaging 5.9% for the lowest tercile and 39.9% for the highest. Diets of high UFB consumers, as compared to low, were significantly less dense in protein, fiber, and seven of the 11 micronutrients assessed and significantly denser in total fat, saturated fat, and total sugar. No associations were found with anthropometric outcomes. High UFB consumers were older and more likely to be living in food insecurity. The most common drivers of commercial UFB consumption were related to child preference, the use of these products as behavior management tools, treats, or gifts, and the sharing of these products by someone else eating them.

Conclusion

High UFB consumption is associated with poor diet quality among 12–35-month-olds in Guédiawaye Department, Senegal. Addressing high UFB consumption during this critical developmental period should be prioritized in young child nutrition research, programming, and policy development.
Risk factors and a new nomogram for predicting brain metastasis from lung cancer: a retrospective study
Authors: Wu Bo , Zhou Yujun , Yang Yong , Zhou Dong
Published: June 2023

Objective

This study aims to establish and validate a new nomogram for predicting brain metastasis from lung cancer by integrating data.

Methods

266 patients diagnosed as lung cancer between 2016 and 2018 were collected from Guangdong Academy of Medical Sciences. The first 70% of patients were designated as the primary cohort and the remaining patients were identified as the internal validation cohort. Univariate and multivariable logistics regression were applied to analyze the risk factors. Independent risk factors were used to construct nomogram. C-index was used to evaluate the prediction effect of nomogram.100 patients diagnosed as lung cancer between 2018 and 2019 were collected for external validation cohorts. The evaluation of nomogram was carried out through the distinction and calibration in the internal validation cohort and external validation cohort.

Results

166 patients were diagnosed with brain metastasis among the 266 patients. The gender, pathological type (PAT), leukocyte count (LCC) and Fibrinogen stage (FibS) were independent risk factors of brain metastasis. A novel nomogram has been developed in this study showed an effective discriminative ability to predict the probability of lung cancer patients with brain metastasis, the C-index was 0.811.

Conclusion

Our research provides a novel model that can be used for predicting brain metastasis of lung cancer patients, thus providing more credible evidence for clinical decision-making.
Functional role of MicroRNA/PI3K/AKT axis in osteosarcoma
Authors: Xiang Yubo , Yang Yingxin , Liu Jia , Yang Xu
Published: June 2023

Osteosarcoma (OS) is a primary malignant bone tumor that occurs in children and adolescents, and the PI3K/AKT pathway is overactivated in most OS patients. MicroRNAs (miRNAs) are highly conserved endogenous non-protein-coding RNAs that can regulate gene expression by repressing mRNA translation or degrading mRNA. MiRNAs are enriched in the PI3K/AKT pathway, and aberrant PI3K/AKT pathway activation is involved in the development of osteosarcoma. There is increasing evidence that miRNAs can regulate the biological functions of cells by regulating the PI3K/AKT pathway. MiRNA/PI3K/AKT axis can regulate the expression of osteosarcoma-related genes and then regulate cancer progression. MiRNA expression associated with PI3K/AKT pathway is also clearly associated with many clinical features. In addition, PI3K/AKT pathway-associated miRNAs are potential biomarkers for osteosarcoma diagnosis, treatment and prognostic assessment. This article reviews recent research advances on the role and clinical application of PI3K/AKT pathway and miRNA/PI3K/AKT axis in the development of osteosarcoma.