bluuebunny/crossref_metadata_2025 huggingface dataset:
Anatomical Variants Identified on Computed Tomography of Oropharyngeal Carcinoma Patients
Authors: Janta Sirorat , Suwannakhan Athikhun , Yurasakpong Laphatrada , Chaiyamoon Arada , Kruepunga Nutmethee , Iwanaga Joe , Tubbs R. Shane , Eiamratchanee Pinthusorn , Paensukyen Tawanrat
Published: April 2023

Background and Objectives: Anatomical variations in the head, neck and chest are common, and are observed as occasional findings on computed tomography (CT). Although anatomical variations are mostly asymptomatic and do not cause any negative influence on the body function, they may jeopardize diagnosis and may be confused with pathological conditions. The presence of variations may also limit surgical access during tumor removal. The aim of this study was to investigate the prevalence of six anatomical variations—os acromiale, episternal ossicles, cervical rib, Stafne bone cavity, azygos lobe and tracheal bronchus—in an open-access computed tomography dataset obtained from oropharyngeal cancer patients. Materials and Methods: A total of 606 upper-chest and neck computed-tomography scans (79.4% male and 20.6% female) were retrospectively investigated. Sex difference was evaluated using the z-test for two proportions. Results: Os acromiale, episternal ossicles, cervical rib, Stafne bone cavity, azygos lobe, and tracheal bronchus were present in 3.1%, 2.2%, 0.2%, 0%, 0.3% and 0.5%, respectively, of all patients. Os acromiale was identified as meso-acromion in 86.6%, and as pre-acromion in 17.4%, of all acromia. Episternal ossicles were present unilaterally in 58.3%, and bilaterally in 41.7%, of all sterna. Only the cervical rib showed a sex difference in prevalence. Conclusions: awareness of these variations is important for radiologists interpreting head, neck and chest CTs; for example, those of oropharyngeal cancer patients. This study also illustrates the applicability of publicly available datasets in prevalence-based anatomical research. While most of the variations investigated in the present study are well-known, the episternal ossicles are not well explored, and need further investigation.
Advances in the Synthesis of Heteroaromatic Hybrid Chalcones
Authors: Mallia Ajay , Sloop Joseph
Published: April 2023

Chalcones continue to occupy a venerated status as scaffolds for the construction of a variety of heterocyclic molecules with medicinal and industrial properties. Syntheses of hybrid chalcones featuring heteroaromatic components, especially those methods utilizing green chemistry principles, are important additions to the preparative methodologies for this valuable class of molecules. This review outlines the advances made in the last few decades toward the incorporation of heteroaromatic components in the construction of hybrid chalcones and highlights examples of environmentally responsible processes employed in their preparation.
A Blooming Season for Natural Polymers and Biopolymers
Authors: Caillol Sylvain
Published: April 2023

The year 2023 is particularly remarkable because we are celebrating the 25th anniversary of the 12 principles of Green Chemistry described in the groundbreaking book Green Chemistry: Theory and Practice co-authored by Paul Anastas and John C [...]
Chemical Characterization of Trichoderma spp. Extracts with Antifungal Activity against Cocoa Pathogens
Authors: Chóez-Guaranda Ivan , Espinoza-Lozano Fernando , Reyes-Araujo Dennys , Romero Christian , Manzano Patricia , Galarza Luis , Sosa Daynet
Published: April 2023

Ecuador is one of the major cocoa producers worldwide, but its productivity has lately been affected by diseases. Endophytic biocontrol agents have been used to minimize pathogenic effects; however, compounds produced by endophytes are minimally understood. This work presents the chemical characterization of the Trichoderma species extracts that proved inhibition against cocoa pathogens. Solid–liquid extraction was performed as a partitioning method using medium with the fungal mycelia of Trichoderma reesei (C2A), Trichoderma sp. (C3A), Trichoderma harzianum (C4A), and Trichoderma spirale (C10) in ethyl acetate individually. The extract of T. spirale (C10) exhibited the growth inhibition (32.97–47.02%) of Moniliophthora perniciosa at 10 µg/mL, while a slight stimulation of Moniliophthora roreri was shown by the extracts of T. reesei (C2A) and T. harzianum (C4A) at higher concentrations. The inhibitory activity could be related to alkaloids, lactones, quinones, flavonoids, triterpenes, and sterols, as indicated by chemical screening and antifungal compounds, such as widdrol, β-caryophyllene, tyrosol, butyl isobutyrate, sorbic acid, palmitic acid, palmitelaidic acid, linoleic acid, and oleic acid, which were identified by gas chromatography–mass spectrometry (GC-MS). The results showed that the extracts, particularly T. spirale (C10), have the potential as biocontrol agents against witches’ broom disease; however, further studies are needed to confirm their effectiveness.
Apoptotic Potential of Glucomoringin Isothiocyanate (GMG-ITC) Isolated from Moringa oleifera Lam Seeds on Human Prostate Cancer Cells (PC-3)
Authors: Abd Karim Nurul Ashikin , Adam Aziza Hussein Bakheit , Jaafaru Mohammed Sani , Rukayadi Yaya , Abdull Razis Ahmad Faizal
Published: April 2023

Inhibition of several protein pathways involved in cancer cell regulation is a necessary key in the discovery of cancer chemotherapy. Moringa oleifera Lam is often used in traditional medicine for the treatment of various illnesses. The plant contains glucomoringin isothiocyanate (GMG-ITC) with therapeutic potential against various cancer cells. Therefore, GMG-ITC was evaluated for its cytotoxicity against the PC-3 prostate cancer cell line and its potential to induce apoptosis. GMG-ITC inhibited cell proliferation in the PC-3 cell line with IC50 value 3.5 µg/mL. Morphological changes as a result of GMG-ITC-induced apoptosis showed chromatin condensation, nuclear fragmentation, and membrane blebbing. Additionally, Annexin V assay showed proportion of cells in early and late apoptosis upon exposure to GMG-ITC in a time-dependent manner. Moreover, GMG-ITC induced a time-dependent G2/M phase arrest, with reduction of 39.1% in the PC-3 cell line. GMG-ITC also activates apoptotic genes including caspase, tumor suppressor gene (p53), Akt/MAPK, and Bax of the proapoptotic Bcl family. Early apoptosis proteins (JNK, Bad, Bcl2, and p53) were significantly upregulated upon GMG-ITC treatment. It is concluded that apoptosis induction was observed in PC-3 cells treated with GMG-ITC. These phenomena suggest that GMG-ITC from M. oleifera seeds could be useful as a future cytotoxic agent against prostate cancer.
Fluorescent Molecular Rotors Based on Hinged Anthracene Carboxyimides
Authors: Ni Yanhai , Fang Wangjian , Olson Mark A.
Published: April 2023

Temperature and viscosity are essential parameters in medicine, environmental science, smart materials, and biology. However, few fluorescent sensor publications mention the direct relationship between temperature and viscosity. Three anthracene carboxyimide-based fluorescent molecular rotors, 1DiAC∙Cl, 2DiAC∙Cl, and 9DiAC∙Cl, were designed and synthesized. Their photophysical properties were studied in various solvents, such as N, N-dimethylacetamide, N, N-dimethylformamide, 1-propanol, ethanol, dimethyl sulfoxide, methanol, and water. Solvent polarizability resulted in a solvatochromism effect for all three rotors and their absorption and emission spectra were analyzed via the Lippert–Mataga equation and multilinear analysis using Kamlet–Taft and Catalán parameters. The rotors exhibited red-shifted absorption and emission bands in solution on account of differences in their torsion angle. The three rotors demonstrated strong fluorescence in a high-viscosity environment due to restricted intramolecular rotation. Investigations carried out under varying ratios of water to glycerol were explored to probe the viscosity-based changes in their optical properties. A good linear correlation between the logarithms of fluorescence intensity and solution viscosity for two rotors, namely 2DiAC∙Cl and 9DiAC∙Cl, was observed as the percentage of glycerol increased. Excellent exponential regression between the viscosity-related temperature and emission intensity was observed for all three investigated rotors.
Evaluation of Antibacterial Activity of Thiourea Derivative TD4 against Methicillin-Resistant Staphylococcus aureus via Destroying the NAD+/NADH Homeostasis
Authors: Hou Yachen , Zhu Sikai , Chen Yamiao , Yu Moxi , Liu Yongsheng , Li Mingkai
Published: April 2023

To develop effective agents to combat bacterial infections, a series of thiourea derivatives (TDs) were prepared and their antibacterial activities were evaluated. Our results showed that TD4 exerted the most potent antibacterial activity against a number of Staphylococcus aureus (S. aureus), including the methicillin-resistant Staphylococcus aureus (MRSA), Staphylococcus epidermidis and Enterococcus faecalis strains, with the minimum inhibitory concentration (MIC) at 2–16 µg/mL. It inhibited the MRSA growth curve in a dose-dependent manner and reduced the colony formation unit in 4× MIC within 4 h. Under the transmission electron microscope, TD4 disrupted the integrity of MRSA cell wall. Additionally, it reduced the infective lesion size and the bacterial number in the MRSA-induced infection tissue of mice and possessed a good drug likeness according to the Lipinski rules. Our results indicate that TD4 is a potential lead compound for the development of novel antibacterial agent against the MRSA infection.
Five-Membered Heterocyclic Sulfonamides as Carbonic Anhydrase Inhibitors
Authors: Angeli Andrea , Paoletti Niccolò , Supuran Claudiu T.
Published: April 2023

The development of heterocyclic derivatives has progressed considerably over the past decades, and many new carbonic anhydrase inhibitors (CAIs) fall into this field. In particular, five-membered heterocyclic sulfonamides have been generally shown to be more effective inhibitors compared to six-membered rings ones. Despite the importance of oxygen and nitrogen five-membered heterocyclic aromatic rings in medicinal chemistry, the installation of sulfonamide moiety on such heterocycles has not received much attention. On the other hand, 1,3,4-thiadiazole/thiadiazoline ring-bearing sulfonamides are the scaffolds which have been widely used in a variety of pharmaceutically important CAIs such as acetazolamide, metazolamide and their many derivatives obtained by using the tail approach. Here, we reviewed the field focusing on the diverse biological activities of these CAIs, such as antiglaucoma, antiepileptic, antitumor and antiinfective properties. This review highlights developments involving five-membered heterocyclic sulfonamides over the last years, with a focus on their pharmacological/clinical applications.
An Overview of Bioactive Phenolic Molecules and Antioxidant Properties of Beer: Emerging Trends
Authors: Nardini Mirella
Published: April 2023

Beer is one of the oldest and most common beverages worldwide. The phenolic contents and antioxidant properties of beer are crucial factors in evaluating its nutritional quality. Special beers brewed with the addition of adjuncts are gaining in consumer preference, in response to demands for healthy food and new gustatory and olfactory stimuli. Many studies recently dealt with functional beers brewed with the addition of adjuncts. This review focuses on bioactive molecules, particularly the composition of phenolic compounds, and the antioxidant activity of beer. The current knowledge concerning the effect of the addition of adjuncts in the form of fruit, vegetables, herbs, and natural foods on the polyphenol content, antioxidant properties, and phenolic profile of beer is reviewed, with an outline of the emerging trends in brewing processes. Future studies need to complete the identification and characterization of the bioactive molecules in beer, as well as studying their absorption and metabolic fate in humans.
Therapeutic Potential of Albumin Nanoparticles Encapsulated Visnagin in MDA-MB-468 Triple-Negative Breast Cancer Cells
Authors: Alsrhani Abdullah , Elderdery Abozer Y. , Alzahrani Badr , Alzerwi Nasser A. N. , Althobiti Maryam Musleh , Rayzah Musaed , Idrees Bandar , Elkhalifa Ahmed M. E. , Subbiah Suresh K. , Mok Pooi Ling
Published: April 2023

Breast cancer is among the most recurrent malignancies, and its prevalence is rising. With only a few treatment options available, there is an immediate need to search for better alternatives. In this regard, nanotechnology has been applied to develop potential chemotherapeutic techniques, particularly for cancer therapy. Specifically, albumin-based nanoparticles are a developing platform for the administration of diverse chemotherapy drugs owing to their biocompatibility and non-toxicity. Visnagin, a naturally derived furanochromone, treats cancers, epilepsy, angina, coughs, and inflammatory illnesses. In the current study, the synthesis and characterization of albumin visnagin (AV) nanoparticles (NPs) using a variety of techniques such as transmission electron microscopy, UV-visible, Fourier transform infrared, energy dispersive X-ray composition analysis, field emission scanning electron microscopy, photoluminescence, X-Ray diffraction, and dynamic light scattering analyses have been carried out. The MTT test, dual AO/EB, DCFH-DA, Annexin-V-FITC/PI, Propidium iodide staining techniques as well as analysis of apoptotic proteins, antioxidant enzymes, and PI3K/Akt/mTOR signaling analysis was performed to examine the NPs’ efficacy to suppress MDA-MB-468 cell lines. The NPs decreased cell viability increased the amount of ROS in the cells, disrupted membrane integrity, decreased the level of antioxidant enzymes, induced cell cycle arrest, and activated the PI3K/Akt/mTOR signaling cascade, ultimately leading to cell death. Thus, AV NPs possesses huge potential to be employed as a strong anticancer therapy alternative.
Insights into the Fluxional Processes of Monomethylcyclohexenyl Manganese Tricarbonyl
Authors: Liang Guangchao , Zhang Min
Published: April 2023

Multiple fluxional processes of 6-monomethylcyclohexenylmanganese tricarbonyl [(6-MeC6H8)Mn(CO)3, complex 1] and 5-monomethylcyclohexenylmanganese tricarbonyl [(5-MeC6H8)Mn(CO)3, complex 2] have been explored using density functional theory (DFT) computations. The contributions of four agostomers—1, 2, 3, and 4—to the (MeC6H8)Mn(CO)3 exchange processes were revealed. The computational results demonstrated that the 1, 2-agostic isomerization only occurred via the η4-diene hydride transition state (TS-1-2, 14.0 kcal/mol), which is consistent with the experimentally proposed high-energy exchange process (16.0 kcal/mol). Excellent agreement is observed (R2 = 0.9862) when comparing the computed and experimentally observed variable temperature 1H NMR chemical shifts. With these results, important insights into the role of agostic interaction in the homogeneous catalysis process could be made, especially with regard to transition metal catalyzed C-H activation.
Green and Sustainable Ultrasound-Assisted Anodic Electrochemical Preparation of Graphene Oxide Dispersions and Their Antioxidant Properties
Authors: Ratova Daria-Maria V. , Mikheev Ivan V. , Chermashentsev Grigoryi R. , Maslakov Konstantin I. , Kottsov Sergei Yu. , Stolbov Dmitrii N. , Maksimov Sergey V. , Sozarukova Madina M. , Proskurnina Elena V. , Proskurnin Mikhail A.
Published: April 2023

A fast method for preparing aqueous graphene oxide (GO) dispersions by electrochemical oxidation of a graphite anode without preliminary intercalation with oxidizing agents is proposed. Ultrasonic probing was used in the modulation mode of ultrasonic waves (work/rest) for more efficient graphite oxidation–exfoliation. It is shown that the 4/2 s mode of ultrasonic modulation is the most effective due to the probe material’s low corrosion while maintaining the optimum synthesis temperature not exceeding 30–35 °C and achieving the best characteristics of the resulting product. Three cases of anodic oxidation of graphite to obtain graphene oxide were considered: (1) a combined cathode–anode compartment, (2) a split cathode–anode salt-bridged compartment, and (3) separated anode compartment with a 3.5 kDa dialysis membrane. It was determined that the approach to synthesis with a divided cathode–anode compartment makes it possible to obtain GO sheets with fewer defects compared to chemical methods or methods with a combined cathode–anode compartment and makes it possible to control the oxidation degree of the material (C:O ratio) by varying the current density. The prepared samples showed good stability for more than six months. The spectral and morphological characteristics were studied. Using chemiluminometry in the luminol/Co(II)/H2O2 system, the antioxidant properties concerning three key reactive oxygen species (H2O2, superoxide anion radical, and hydroxyl radical) were demonstrated. It was also shown that the prepared GO dispersions do not induce lipid and phospholipid peroxidation.
Biosynthesis of Gold and Silver Nanoparticles Using Phytochemical Compounds
Authors: Zuhrotun Ade , Oktaviani Dede Jihan , Hasanah Aliya Nur
Published: April 2023

Gold and silver nanoparticles are nanoparticles that have been widely used in various fields and have shown good benefits. The method of nanoparticle biosynthesis utilizing plant extracts, also known as green synthesis, has become a promising method considering the advantages it has compared to other synthesis methods. This review aims to give an overview of the phytochemical compounds in plants used in the synthesis of gold and silver nanoparticles, the nanoparticle properties produced using plant extracts based on the concentration and structure of phytochemical compounds, and their applications. Phytochemical compounds play an important role as reducing agents and stabilizers in the stages of the synthesis of nanoparticles. Polyphenol compounds, reducing sugars, and proteins are the main phytochemical compounds that are responsible for the synthesis of gold and silver nanoparticles. The concentration of phytochemical compounds affects the physical properties, stability, and activity of nanoparticles. This is important to know to be able to overcome limitations in controlling the physical properties of the nanoparticles produced. Based on structure, the phytochemical compounds that have ortho-substituted hydroxyl result in a smaller size and well-defined shape, which can lead to greater activity and stability. Furthermore, the optimal condition of the biosynthesis process is required to gain a successful reaction that includes setting the metal ion concentration, temperature, reaction time, and pH.
Enhancement Mechanism of Pt/Pd-Based Catalysts for Oxygen Reduction Reaction
Authors: Zhang Xinqun , Wang Jiaqi , Zhao Yang
Published: April 2023

The oxygen reduction reaction (ORR) is one of the key catalytic reactions for hydrogen fuel cells, biofuel cells and metal–air cells. However, due to the complex four-electron catalytic process, the kinetics of the oxygen reduction reaction are sluggish. Platinum group metal (PGM) catalysts represented by platinum and palladium are considered to be the most active ORR catalysts. However, the price and reserves of Pt/Pd are major concerns and issues for their commercial application. Improving the catalytic performance of PGM catalysts can effectively reduce their loading and material cost in a catalytic system, and they will be more economical and practical. In this review, we introduce the kinetics and mechanisms of Pt/Pd-based catalysts for the ORR, summarize the main factors affecting the catalytic performance of PGMs, and discuss the recent progress of Pt/Pd-based catalysts. In addition, the remaining challenges and future prospects in the design and improvement of Pt/Pd-based catalysts of the ORR are also discussed.
A Physical Model to Describe the Motion Behavior of BNNSs under Nanosecond Pulses
Authors: Zhao Liang , Zhou Lin , Jin Lin
Published: April 2023

This paper presents a physical model that provides a comprehensive understanding of the motion behavior of boron nitride nanosheets (BNNSs) immersed in ultrapure deionized water and subjected to a series of nanosecond pulses. In a study conducted by Y. Mi et al. The authors explored the global alignment behavior of BNNSs and fitted the experimental data with an exponential decay function. However, this function lacks clear physical mechanisms and the significance of the fitting parameters remains unclear. To address this issue, we have developed a kinetic model that explicitly describes the underlying physical mechanisms. Furthermore, we propose a simplified mathematical model that not only predicts the displacement of BNNSs but also estimates the total time, velocity, and acceleration of the motion process.
Relationship between the Mediterranean Diet Score in Pregnancy and the Incidence of Asthma at 4 Years of Age: The Japan Environment and Children’s Study
Authors: Nakano Kaita , Kuraoka Shohei , Oda Masako , Ohba Takashi , Mitsubuchi Hiroshi , Nakamura Kimitoshi , Katoh Takahiko ,   the Japan Environment and Children’s Study (JECS) Group
Published: April 2023

Several scoring methods for the Mediterranean diet, which is considered as a healthy diet, are available, but studies that have compared more than one of these scores are rare. In addition, the applicability of Mediterranean diet scoring has not been sufficiently examined outside of Mediterranean regions. We collected data on the Mediterranean diet during pregnancy and the incidence of type 1 allergies in offspring from the Japan Environment and Children’s Study. Using multiple Mediterranean diet scoring methods, we analyzed the effect of adherence to the Mediterranean diet in pregnancy on the allergies of the offspring. Overall, 46,532 pairs of mothers and children were analyzed. In Japan, a high adherence to the Mediterranean diet during pregnancy was associated with a lower incidence of asthma in the offspring (odds ratio: 0.896, 95% confidence interval: 0.835, 0.962). Furthermore, we found that the selection of the Mediterranean diet scoring method and the setting of the reference value significantly altered the results. Our findings suggest that an appropriate selection of scoring methods and a reference value for food items are important to analyze the effects of adherence to the Mediterranean diet inside and outside of Mediterranean regions.
Hydroxytyrosol Interference with Inflammaging via Modulation of Inflammation and Autophagy
Authors: Velotti Francesca , Bernini Roberta
Published: April 2023

Inflammaging refers to a chronic, systemic, low-grade inflammation, driven by immune (mainly macrophages) and non-immune cells stimulated by endogenous/self, misplaced or altered molecules, belonging to physiological aging. This age-related inflammatory status is characterized by increased inflammation and decreased macroautophagy/autophagy (a degradation process that removes unnecessary or dysfunctional cell components). Inflammaging predisposes to age-related diseases, including obesity, type-2 diabetes, cancer, cardiovascular and neurodegenerative disorders, as well as vulnerability to infectious diseases and vaccine failure, representing thus a major target for anti-aging strategies. Phenolic compounds—found in extra-virgin olive oil (EVOO)—are well known for their beneficial effect on longevity. Among them, hydroxytyrosol (HTyr) appears to greatly contribute to healthy aging by its documented potent antioxidant activity. In addition, HTyr can modulate inflammation and autophagy, thus possibly counteracting and reducing inflammaging. In this review, we reference the literature on pure HTyr as a modulatory agent of inflammation and autophagy, in order to highlight its possible interference with inflammaging. This HTyr-mediated activity might contribute to healthy aging and delay the development or progression of diseases related to aging.
Taq-Polymerase Stop Assay to Determine Target Selectivity of G4 Ligands in Native Promoter Sequences of MYC, TERT, and KIT Oncogenes
Authors: Chashchina Galina V. , Tevonyan Liana L. , Beniaminov Artemy D. , Kaluzhny Dmitry N.
Published: April 2023

Computational and high-throughput experimental methods predict thousands of potential quadruplex sequences (PQSs) in the human genome. Often these PQSs contain more than four G-runs, which introduce additional uncertainty into the conformational polymorphism of the G4 DNA. G4-specific ligands, which are currently being actively developed as potential anticancer agents or tools for studying G4 structures in genomes, may preferentially bind to specific G4 structures over the others that can be potentially formed in the extended G-rich genomic region. We propose a simple technique that identifies the sequences that tend to form G4 in the presence of potassium ions or a specific ligand. Thermostable DNA Taq-polymerase stop assay can detect the preferential position of the G4 –ligand binging within a long PQS-rich genomic DNA fragment. This technique was tested for four G4 binders PDS, PhenDC3, Braco-19, and TMPyP4 at three promoter sequences of MYC, KIT, and TERT that contain several PQSs each. We demonstrate that the intensity of polymerase pausing reveals the preferential binding of a ligand to particular G4 structures within the promoter. However, the strength of the polymerase stop at a specific site does not always correlate with the ligand-induced thermodynamic stabilization of the corresponding G4 structure.
Efficient mRNA Delivery with mRNA Lipoplexes Prepared Using a Modified Ethanol Injection Method
Authors: Tang Min , Sagawa Ayane , Inoue Nodoka , Torii Satomi , Tomita Kana , Hattori Yoshiyuki
Published: April 2023

Messenger RNA (mRNA)-based therapies are a novel class of therapeutics used in vaccination and protein replacement therapies for monogenic diseases. Previously, we developed a modified ethanol injection (MEI) method for small interfering RNA (siRNA) transfection, in which cationic liposome/siRNA complexes (siRNA lipoplexes) were prepared by mixing a lipid-ethanol solution with a siRNA solution. In this study, we applied the MEI method to prepare mRNA lipoplexes and evaluated the in vitro and in vivo protein expression efficiencies. We selected six cationic lipids and three neutral helper lipids to generate 18 mRNA lipoplexes. These were composed of cationic lipids, neutral helper lipids, and polyethylene glycol-cholesteryl ether (PEG-Chol). Among them, mRNA lipoplexes containing N-hexadecyl-N,N-dimethylhexadecan-1-aminium bromide (DC-1-16) or 11-((1,3-bis(dodecanoyloxy)-2-((dodecanoyloxy)methyl) propan-2-yl) amino)-N,N,N-trimethyl-11-oxoundecan-1-aminium bromide (TC-1-12) with 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) and PEG-Chol exhibited high protein expression in cells. Furthermore, mRNA lipoplexes composed of DC-1-16, DOPE, and PEG-Chol exhibited high protein expression in the lungs and spleen of mice after systemic injection and induced high antigen-specific IgG1 levels upon immunization. These results suggest that the MEI method can potentially increase the efficiency of mRNA transfection, both in vitro and in vivo.
Clustering in Oxygen Nuclei and Spectator Fragments in 16O–16O Collisions at the LHC
Authors: Svetlichnyi Aleksandr , Savenkov Savva , Nepeivoda Roman , Pshenichnov Igor
Published: April 2023

A new version of the Abrasion–Ablation Monte Carlo for Colliders model with the Minimum Spanning Tree clusterization algorithm (AAMCC-MST) is used to simulate 16O–16O collisions at the LHC, accounting for the presence of alpha-clustered states in 16O. The yields of He, Li, Be, B, C and N spectator nuclei are calculated taking into account the pre-equilibrium clusterization of spectator matter and short-range correlations (SRC) between nucleons in 16O. The impact of α-clustering and SRC on the production of spectator neutrons and deuterons is investigated. The results on the production of spectator nucleons and fragments can help in evaluating the performance of Zero Degree Calorimeters in future studies of 16O–16O collisions at the LHC.
The Contribution of Epigenetics to Evolutionary Adaptation in Zingiber kawagoii Hayata (Zingiberaceae) Endemic to Taiwan
Authors: Li Yi-Shao , Liao Pei-Chun , Chang Chung-Te , Hwang Shih-Ying
Published: April 2023

We epigenotyped 211 individuals from 17 Zingiber kawagoii populations using methylation-sensitive amplification polymorphism (MSAP) and investigated the associations of methylated (mMSAP) and unmethylated (uMSAP) loci with 16 environmental variables. Data regarding genetic variation based on amplified fragment length polymorphism (AFLP) were obtained from an earlier study. We found a significant positive correlation between genetic and epigenetic variation. Significantly higher mean mMSAP and uMSAP uHE (unbiased expected heterozygosity: 0.223 and 0.131, respectively, p < 0.001) per locus than that estimated based on AFLP (uHE = 0.104) were found. Genome scans detected 10 mMSAP and 9 uMSAP FST outliers associated with various environmental variables. A significant linear fit for 11 and 12 environmental variables with outlier mMSAP and uMSAP ordination, respectively, generated using full model redundancy analysis (RDA) was found. When conditioned on geography, partial RDA revealed that five and six environmental variables, respectively, were the most important variables influencing outlier mMSAP and uMSAP variation. We found higher genetic (average FST = 0.298) than epigenetic (mMSAP and uMSAP average FST = 0.044 and 0.106, respectively) differentiation and higher genetic isolation-by-distance (IBD) than epigenetic IBD. Strong epigenetic isolation-by-environment (IBE) was found, particularly based on the outlier data, controlling either for geography (mMSAP and uMSAP βE = 0.128 and 0.132, respectively, p = 0.001) or for genetic structure (mMSAP and uMSAP βE = 0.105 and 0.136, respectively, p = 0.001). Our results suggest that epigenetic variants can be substrates for natural selection linked to environmental variables and complement genetic changes in the adaptive evolution of Z. kawagoii populations.
Study of the Plasticization Effect of 1-Ethyl-3-methylimidazolium Acetate in TPS/PVA Biodegradable Blends Produced by Melt-Mixing
Authors: Castro Jennifer M. , Montalbán Mercedes G. , Domene-López Daniel , Martín-Gullón Ignacio , García-Quesada Juan C.
Published: April 2023

The first step towards the production and marketing of bioplastics based on renewable and sustainable materials is to know their behavior at a semi-industrial scale. For this reason, in this work, the properties of thermoplastic starch (TPS)/polyvinyl alcohol (PVA) films plasticized by a green solvent, as the 1-ethyl-3-methylimidazolium acetate ([Emim+][Ac−]) ionic liquid, produced by melt-mixing were studied. These blends were prepared with a different content of [Emim+][Ac−] (27.5–42.5 %wt.) as a unique plasticizer. According to the results, this ionic liquid is an excellent plasticizer due to the transformation of the crystalline structure of the starch to an amorphous state, the increase in flexibility, and the drop in Tg, as the [Emim+][Ac−] amount increases. These findings show that the properties of these biomaterials could be modified in the function of [Emim+][Ac−] content in the formulations of TPS, depending on their final use, thus becoming a functional alternative to conventional polymers.
Benefits and Limitations of Artificial Neural Networks in Process Chromatography Design and Operation
Authors: Mouellef Mourad , Vetter Florian Lukas , Strube Jochen
Published: April 2023

Due to the progressive digitalization of the industry, more and more data is available not only as digitally stored data but also as online data via standardized interfaces. This not only leads to further improvements in process modeling through more data but also opens up the possibility of linking process models with online data of the process plants. As a result, digital representations of the processes emerge, which are called Digital Twins. To further improve these Digital Twins, process models in general, and the challenging process design and development task itself, the new data availability is paired with recent advancements in the field of machine learning. This paper presents a case study of an ANN for the parameter estimation of a Steric Mass Action (SMA)-based mixed-mode chromatography model. The results are used to exemplify, discuss, and point out the effort/benefit balance of ANN. To set the results in a wider context, the results and use cases of other working groups are also considered by categorizing them and providing background information to further discuss the benefits, effort, and limitations of ANNs in the field of chromatography.
Struggling to Restore a Lost Identity: Hanshan Deqing’s 憨山德清 (1546–1623) Reforms at Nanhua Temple 南華寺, 1600–1610
Authors: Zhang Dewei
Published: April 2023

During the ten years from Wanli 28 to 38 (1600–1610), Hanshan Deqing, then an exiled leading Buddhist master, managed to launch large-scale reforms in Nanhua temple in an attempt to reinvigorate the ancestral temple of Chan Buddhism. Strategically significant though it was, this effort proved eventful and finally came to a tragic end, including the suicide of the temple’s incumbent abbot. How deeply the process of the reforms and their significance can be understood hinges upon the extent to which two puzzles can be tackled. First, how could it have been possible for Deqing, as an exile, to initiate the reforms in such a significant temple in the first place? And how and why did Deqing’s efforts evolve into such a life-and-death confrontation? Keeping these questions in mind, this article reveals how Deqing was able to mobilize resources for initial success by adjusting his strategies according to the situation; how his efforts were conditioned both by domestic situations on the local, regional, and national levels, respectively, and by international elements that characterized the dawn of the global age; and how the reform efforts failed halfway amid the escalating tensions between the new group led by Deqing and Nanhua’s existing monks. This study highlights both the uniqueness of Buddhism in the often-overlooked Lingnan region—which, to a large part, determined the fate of Deqing’s reform—and the vitality and fragility of the ongoing late-Ming Buddhist renewal.
Insights into the Magmatic Feeding System of the 2021 Eruption at Cumbre Vieja (La Palma, Canary Islands) Inferred from Gravity Data Modeling
Authors: Montesinos Fuensanta G. , Sainz-Maza Sergio , Gómez-Ortiz David , Arnoso José , Blanco-Montenegro Isabel , Benavent Maite , Vélez Emilio , Sánchez Nieves , Martín-Crespo Tomás
Published: April 2023

This study used spatiotemporal land gravity data to investigate the 2021 eruption that occurred in the Cumbre Vieja volcano (La Palma, Canary Islands). First, we produced a density model by inverting the local gravity field using data collected in July 2005 and July 2021. This model revealed a low-density body beneath the western flank of the volcano that explains a highly fractured and altered structure related to the active hydrothermal system. Then, we retrieved changes in gravity and GNSS vertical displacements from repeated measurements made in a local network before (July 2021) and after (January 2022) the eruption. After correcting the vertical surface displacements, the gravity changes produced by mass variation during the eruptive process were used to build a forward model of the magmatic feeding system consisting of dikes and sills based on an initial model defined by the paths of the earthquake hypocenters preceding the eruption. Our study provides a final model of the magma plumbing system, which establishes a spatiotemporal framework tracing the path of magma ascent from the crust–mantle boundary to the surface from 11–19 September 2021, where the shallowest magma path was strongly influenced by the low-density body identified in the inversion process.
Monitoring Glacier Lake Outburst Flood (GLOF) of Lake Merzbacher Using Dense Chinese High-Resolution Satellite Images
Authors: Gu Changjun , Li Suju , Liu Ming , Hu Kailong , Wang Ping
Published: April 2023

Establishing an effective real-time monitoring and early warning system for glacier lake outburst floods (GLOFs) requires a full understanding of their occurrence mechanism. However, the harsh conditions and hard-to-reach locations of these glacial lakes limit detailed fieldwork, making satellite imagery a critical tool for monitoring. Lake Mercbacher, an ice-dammed lake in the central Tian Shan mountain range, poses a significant threat downstream due to its relatively high frequency of outbursts. In this study, we first monitored the daily changes in the lake area before the 2022 Lake Mercbacher outburst. Additionally, based on historical satellite images from 2014 to 2021, we calculated the maximum lake area (MLA) and its changes before the outburst. Furthermore, we extracted the proportion of floating ice and water area during the period. The results show that the lake area of Lake Mercbacher would first increase at a relatively low speed (0.01 km2/day) for about one month, followed by a relatively high-speed increase (0.04 km2/day) until reaching the maximum, which would last for about twenty days. Then, the lake area would decrease slowly until the outburst, which would last five days and is significant for early warning. Moreover, the floating ice and water proportion provides more information about the outburst signals. In 2022, we found that the floating ice area increased rapidly during the early warning stage, especially one day before the outburst, accounting for about 50% of the total lake area. Historical evidence indicates that the MLA shows a decreasing trend, and combining it with the outburst date and climate data, we found that the outburst date shows an obvious advance trend (6 days per decade) since 1902, caused by climate warming. Earlier melting results in an earlier outburst. This study provides essential references for monitoring Lake Mercbacher GLOFs and building an effective early warning system.
Robotic Ultrasonic Testing Technology for Aero-Engine Blades
Authors: Ma Pengzhi , Xu Chunguang , Xiao Dingguo
Published: April 2023

With the rapid development of the aerospace industry, the quality inspection of complex curved components, such as aero-engine blades, is becoming increasingly strict. In contrast with other NDT methods, ultrasonic testing is easier to automate, while offering higher accuracy and efficiency in thickness measuring. To solve the challenge of the automated NDT inspection of aero-engine blades, in this study, an ultrasonic inspection system with a six degree of freedom (DOF) was proposed for industrial robots. Additionally, a defect detection model and a thickness detection method were proposed for the robotic ultrasonic inspection system, based on the thickness variation of the aero-engine blade. Through the quantitative analysis on engine blades with prefabricated defects and curved test blocks with stepped thicknesses, it can be concluded that our system is able to achieve high accuracy in defect detection and thickness measurement.
Design of Planar Differential Microphone Array Beampatterns with Controllable Mainlobe Beamwidth and Sidelobe Level
Authors: Wang Xianghui , Li Mei , Zhao Yingke , Wang Jiao , Tan Xin
Published: April 2023

The differential microphone array, or differential beamformer, has attracted much attention for its frequency-invariant beampattern, high directivity factor and compact size. In this work, the design of differential beamformers with small inter-element spacing planar microphone arrays is concerned. In order to exactly control the main lobe beamwidth and sidelobe level and obtain minimum main lobe beamwidth with a given sidelobe level, we design the desired beampattern by applying the Chebyshev polynomials at first, via exploiting the structure of the frequency-independent beampattern of a theoretical Nth-order differential beamformer. Next, the so-called null constrained and least square beamformers, which can obtain approximately frequency-invariant beampattern at relatively low frequencies and can be steered to any direction without beampattern distortion, are proposed based on planar microphone arrays to approximate the designed desired beampatterns. Then, for dealing with the white noise amplification at low-frequency bands and beampattern divergence problems at high-frequency bands of the null constrained and least square beamformers, the so-called minimum norm and combined solutions are deduced, which can compromise among the white noise gain, directivity factor and beampattern distortion flexibly. Preliminary simulation results illustrate the properties and advantages of the proposed differential beamformers.
Mammalian Cell-Growth Monitoring Based on an Impedimetric Sensor and Image Processing within a Microfluidic Platform
Authors: Podunavac Ivana , Knežić Teodora , Djisalov Mila , Omerovic Nejra , Radovic Marko , Janjušević Ljiljana , Stefanovic Dimitrije , Panic Marko , Gadjanski Ivana , Radonic Vasa
Published: April 2023

In recent years, advancements in microfluidic and sensor technologies have led to the development of new methods for monitoring cell growth both in macro- and micro-systems. In this paper, a microfluidic (MF) platform with a microbioreactor and integrated impedimetric sensor is proposed for cell growth monitoring during the cell cultivation process in a scaled-down simulator. The impedimetric sensor with an interdigitated electrode (IDE) design was realized with inkjet printing and integrated into the custom-made MF platform, i.e., the scaled-down simulator. The proposed method, which was integrated into a simple and rapid fabrication MF system, presents an excellent candidate for the scaled-down analyses of cell growths that can be of use in, e.g., optimization of the cultivated meat bioprocess. When applied to MRC-5 cells as a model of adherent mammalian cells, the proposed sensor was able to precisely detect all phases of cell growth (the lag, exponential, stationary, and dying phases) during a 96-h cultivation period with limited available nutrients. By combining the impedimetric approach with image processing, the platform enables the real-time monitoring of biomasses and advanced control of cell growth progress in microbioreactors and scaled-down simulator systems.
Transforming Industrial Manipulators via Kinesthetic Guidance for Automated Inspection of Complex Geometries
Authors: Loukas Charalampos , Vasilev Momchil , Zimmerman Rastislav , Vithanage Randika K. W. , Mohseni Ehsan , MacLeod Charles N. , Lines David , Pierce Stephen Gareth , Williams Stewart , Ding Jialuo , Burnham Kenneth , Sibson Jim , O’Hare Tom , Grosser Michael R.
Published: April 2023

The increased demand for cost-efficient manufacturing and metrology inspection solutions for complex-shaped components in High-Value Manufacturing (HVM) sectors requires increased production throughput and precision. This drives the integration of automated robotic solutions. However, the current manipulators utilizing traditional programming approaches demand specialized robotic programming knowledge and make it challenging to generate complex paths and adapt easily to unique specifications per component, resulting in an inflexible and cumbersome teaching process. Therefore, this body of work proposes a novel software system to realize kinesthetic guidance for path planning in real-time intervals at 250 Hz, utilizing an external off-the-shelf force–torque (FT) sensor. The proposed work is demonstrated on a 500 mm2 near-net-shaped Wire–Arc Additive Manufacturing (WAAM) complex component with embedded defects by teaching the inspection path for defect detection with a standard industrial robotic manipulator in a collaborative fashion and adaptively generating the kinematics resulting in the uniform coupling of ultrasound inspection. The utilized method proves superior in performance and speed, accelerating the programming time using online and offline approaches by an estimate of 88% to 98%. The proposed work is a unique development, retrofitting current industrial manipulators into collaborative entities, securing human job resources, and achieving flexible production.
Adapting to Crisis: The Governance of Public Services for Migrants and Refugees during COVID-19 in Four European Cities
Authors: Zardo Federica , Rössl Lydia , Khoury Christina
Published: April 2023

The lack of access to basic services played a big part among the key effects of COVID-19 on migrants and refugees. This paper examines the governance dynamics behind public services for migrants and refugees to understand how COVID-19 has impacted them and what accounts for different levels of adaptive capacity. It employs a mixed methods approach, using egocentric network analysis and qualitative interviews to compare the service ecosystems in four European cities from 2020 to 2022 (Birmingham, Larissa, Malaga, and Palermo). The paper explores the impact of two conditions on the service ecosystems’ ability to adapt to the pandemic: the structure of governance and the presence of dynamic capabilities. We argue that the ability of local governments to manage pandemic challenges is highly dependent on the formal distribution of comprehensive competences across various levels (the structure of governance), and the quality of network cooperation between different administrations and civil society (dynamic capabilities). Our analysis reveals that while both conditions are critical for the level of adaptive capacity in public services’ provision, the structure of governance is more likely to act as a constraint or trigger for coping strategies.
Consequential Life Cycle Assessment of Grain and Oilseed Crops: Review and Recommendations
Authors: Bamber Nicole , Turner Ian , Dutta Baishali , Heidari Mohammed Davoud , Pelletier Nathan
Published: April 2023

The field crop industry in Canada is a source of both significant economic benefits and environmental impacts. Environmental impacts include land and energy use, as well as greenhouse gas (GHG) and other emissions. Impacts also accrue upstream of the field in the product supply chain, from the production of such inputs as fertilizers and pesticides. There are currently two types of environmental life cycle assessment (LCA)—attributional LCA (ALCA) and consequential LCA (CLCA)—that may be used to study the life cycle impacts of products such as field crops. ALCA is a retrospective methodology that presents a snapshot of average, “status quo” conditions. CLCA is a prospective methodology that presents the potential implications of changes in a product system, including any associated market-mediated changes in supply or demand in other product systems. Thus, CLCAs can be used to assess large-scale changes in the field crop industry, including its relationship to other sectors and processes, such as the production of biofuel or of food for both human and animal consumption. The aim of this paper is to review and curate the knowledge derived through published CLCA studies that assessed the impacts of changes to field crop production systems on the life cycle resource use and emissions associated with the agricultural products, with a focus on their relevance to temperate climate conditions. The current study also highlights how previous studies, including ALCAs and farm management recommendations, can be used to inform the changes that should be studied using CLCA. The main challenges to conducting CLCAs include identifying the system boundaries, marginal products and processes that would be impacted by changes to field crop production. Marginal markets and product systems to include can be determined using economic equilibrium models, or information from local experts and industry reports. In order to conduct ISO-compliant CLCAs, it is necessary to include multiple relevant environmental impact categories, and to perform robust data quality and uncertainty analyses.