bluuebunny/crossref_metadata_2025 huggingface dataset:
The Effects of Privileging Moral or Performance Character Development in Urban Adolescents
Authors: Seider Scott , Novick Sarah , Gomez Jessica
Published: June 2013

This study compared the effects of emphasizing moral character development or performance character development at three high-performing, high-poverty urban middle schools. Performance character consists of the qualities that allow individuals to regulate their thoughts and actions in ways that support achievement in a particular endeavor. Moral character consists of the qualities relevant to striving for ethical behavior in one’s relationships with other individuals and communities. Using a quasi-experimental research design, the authors found that early adolescents attending a school emphasizing moral character development through ethical philosophy programming demonstrated significantly higher levels of integrity over the course of the 2010 to 2011 academic year than their peers at two matched comparison schools ( N = 544). However, the early adolescents attending the comparison schools—which emphasized performance character development through advisory programming—demonstrated significantly higher levels of perseverance and community connectedness over the course of the academic year. These divergent outcomes across the three schools offer useful implications to educators, researchers, and policy-makers about the different effects of privileging a particular dimension of character education.
Multidimensional Conflict and Organizational Performance
Authors: Flink Carla M.
Published: June 2013

Conflict is part of every organization. Scholars have studied the effects of conflict on organization dynamics and their outputs. Literature suggests that not all conflict is detrimental for organizations—some conflict actually helps bolster and refresh organizations. One concern for organizations is the vertical strategic alignment of management strategies. Vertical cohesion or conflict impacts an organization’s ability to reach optimal performance. In the setting of English local governments, this study uses vertical strategic differences among two levels of management as the measure of conflict in organizations to examine, one, how it impacts organization performance, and two, if conflict has a nonlinear relationship with performance. Results indicate that conflict on single strategies has no bearing on organization performance. Total strategy or multidimensional conflict, however, negatively impacts performance. There is little support for a nonlinear relationship between conflict and performance. Further analyses indicate that the negative impact of conflict is amplified for smaller organizations.
Response of savanna gross primary productivity to interannual variability in rainfall
Authors: Kanniah Kasturi Devi , Beringer Jason , Hutley Lindsay B.
Published: June 2013

Studying the temporal pattern of savanna gross primary productivity (GPP) is essential for predicting the response of the biome to global environmental changes. In this study, MODIS satellite data coupled with eddy covariance based flux measurements were used to estimate GPP using a remote sensing based light use efficiency model across a significant rainfall gradient in the Northern Territory (NT) region of Australia. Closed forest that occurred in wet and often fireproof environments assimilated (GPP) 4–6 times more carbon than grasslands and Acacia woodlands that grow in arid environments (<600 mm annual rainfall). However, due to their small spatial extent, closed forests contributed <0.5% of the regional budget compared to savanna woodlands (86%) and grasslands (32%). Annual rainfall was found to exert a significant influence on GPP for different vegetation types except for closed forest which was less sensitive to above-average rainfall. Interannual variability in GPP showed that arid ecosystems had a higher variation (>20%) compared to woodlands and forest (∼5%). This variation in GPP was correlated with that of rainfall (R2 = 0.88, p<0.05). Analysis of the impact of wettest and driest years on GPP showed a strong positive correlation between the magnitude of the relative maxima in rainfall and maxima in GPP (R2 = 0.89, p<0.05). In contrast, the relative rainfall minima exhibited an insignificant relationship with relative GPP minima (R2 = 0.45, p = 0.07). These findings provide valuable information on the carbon uptake across the savanna biome and show the sensitivity of different vegetation systems to rainfall, a variable that may change in quantity and variability with projected climate change. Such data also show regions of high levels of carbon that could be linked with savanna management to protect the resources in the Australian savannas.
Experiments in the Laboratories of Democracy
Authors: Feldman Maryann P. , Lanahan Lauren , Lendel Iryna V.
Published: June 2013

State initiatives that build innovation capacity by supporting local academic research, attracting eminent scholars, and building research excellence have become prominent among the 50 states over the past 30 years. This article focuses on three programs: University Research Grants, Eminent Scholars, and Centers of Excellence. We include examples for each of the state programs and trace the historical evolution of program attributes. Our objectives are to differentiate program attributes to improve understanding of state science initiatives and to begin to assess how programs contribute to the ultimate goal of creating economic growth. Our empirical analysis demonstrates evidence of the long-term impact of these three programs in building state innovative capacity. The article concludes by outlining how these data may be used in future analyses.
Link and effect model for performance improvement of railway infrastructure
Authors: Stenström Christer , Parida Aditya , Galar Diego , Kumar Uday
Published: June 2013

Railway traffic has increased over the last decade due to its fuel efficiency and the need to reduce emissions. The railway infrastructure performance needs to be measured to allow assets to be managed effectively against set objectives. Various systems are used to collect and store data on traffic, failures, inspections, track quality, etc. However, these systems are often used in an ad hoc manner, partly because of the weaknesses of traditional performance measurement systems. This paper proposes a link and effect model which is focused on the areas of continuous improvement, the key elements of strategic planning and on the underlying factors responsible for the railway performance. The model provides information on the performance of railway systems and components, and how they are linked to each other and to the overall objectives, thereby facilitating proactive decision-making. The model is applied in a case study on the Iron Ore Line in Sweden. The performance of a section of the line is studied in terms of failures, train delays and repair times, and ranked through a risk matrix and composite indicator.
Detection and identification of first and second heart sounds using empirical mode decomposition
Authors: Bajelani Kourosh , Navidbakhsh Mahdi , Behnam Hamid , Doyle John D , Hassani Kamran
Published: June 2013

We present a novel, low complexity method for the detection of the first and second of heart sounds (S1 and S2, respectively) and the periods of systole and diastole without using an electrocardiogram reference. The algorithm uses a technique called empirical mode decomposition to produce intensity envelopes of the main heart sounds in the time domain. The performance of the algorithm was evaluated using 14,000 cardiac periods from 100 normal and abnormal digital phonocardiographic recordings. The sensitivity of the detection method was 88.3% for both S1 and S2, and the precision (positive predictive value) was 95.8% for both S1 and S2.
Incentivizing Wellness in the Workplace
Authors: Tannenbaum David , Valasek Chad J. , Knowles Eric D. , Ditto Peter H.
Published: June 2013

Companies often provide incentives for employees to maintain healthy lifestyles. These incentives can take the form of either discounted premiums for healthy-weight employees (“carrot” policies) or increased premiums for overweight employees (“stick” policies). In the three studies reported here, we demonstrated that even when stick and carrot policies are formally equivalent, they do not necessarily convey the same information to employees. Stick but not carrot policies were viewed as reflecting negative company attitudes toward overweight employees (Study 1a) and were evaluated especially negatively by overweight participants (Study 1b). This was true even when overweight employees paid less money under the stick than under the carrot policy. When acting as policymakers (Study 2), participants with high levels of implicit overweight bias were especially likely to choose stick policies—often on the grounds that such policies were cost-effective—even when doing so was more costly to the company. Policymakers should realize that the framing of incentive programs can convey tacit, and sometimes stigmatizing, messages.
Progressive improvement of patient and renal survival and reduction of morbidity over time in patients with lupus nephritis (LN) followed for 20 years
Authors: Moroni G , Quaglini S , Gallelli B , Banfi G , Messa P , Ponticelli C
Published: June 2013

Whether the long-term patient and renal survival of those diagnosed with lupus nephritis (LN) has improved over the decades is still debated. Eighty-nine patients diagnosed between 1968 and 1990 entered this study and their outcome was evaluated after 20 years. At presentation 54% of patients had class IV LN, 39.3% had renal insufficiency and 59.5% had nephrotic syndrome. Patients were divided into two groups: Group 1 consisted of 30 patients diagnosed between 1968 and 1980; Group 2 consisted of 59 patients diagnosed between 1981 and 1990. In Group 1 patient survival at 20 years was 84% versus 95% in Group 2 ( p = 0.05). Survivals without end-stage renal failure were respectively 75% and 84% at 20 years ( p = 0.05). Survivals without severe infection at 20 years were 44% in Group 1 and 66.5% in Group 2 ( p = 0.02). Survivals without cardiovascular events at 20 years were: 53% in Group 1 and 90% in Group 2 ( p = 0.005). At presentation, patients in Group 1 had higher serum creatinine (1.96 vs 1.15 mg/dl, p = 0.01), higher activity index (8 vs 5.5, p = 0.01), lower hematocrit (31% v s6%, p = 0.008) and lower serum C4 levels ( p = 0.04) than Group 2 patients. Patients in Group 1 also received less frequent methylprednisolone pulses (43% v s81%, p = 0.0006). In Italian patients with LN, long-term life expectancy and renal survival progressively improved over the decades, while morbidity progressively declined. An earlier referral and refinement of therapy achieved this goal.
Traditional Heart Failure Medications and Sudden Cardiac Death Prevention
Authors: Al Chekakie M. Obadah
Published: June 2013

Sudden cardiac death (SCD) is still a major public health issue with an estimated annual incidence ranging from 184,000 to > 400,000 per year. The ACC/AHA/ESC 2006 guidelines define SCD as “death from an unexpected circulatory arrest, usually due to a cardiac arrhythmia occurring within an hour of the onset of symptoms”. A recent study of sudden cardiac death using multiple sources of ascertainment found that coronary artery disease was present in more than 50% of patients older than 35 years who died suddenly and underwent autopsy. Antiarrhythmic drugs have failed to show any mortality benefit even when compared to placebo or implantable cardiovertor defibrillators (ICDs). While patients with systolic heart failure are at higher risk of dying suddenly, most of the patients experiencing sudden cardiac death have left ventricular ejection fraction (LVEF) > 50%. β-blockers, Angiotensin enzymes (ACE) inhibitors as well as aldosterone antagonists prevent ischemia and remodelling in the left ventricle especially in post myocardial infarction (MI) patients and in patients with systolic heart failure. This article will review the data on the effects of traditional heart failure medications, especially β-blockers, Renin Angiotensin system blockers, as well as Statin therapy on sudden cardiac death in post MI patients and in patients with systolic heart failure.
On the microscopic definitions of the dislocation density tensor
Authors: Mandadapu Kranthi K , Jones Reese E , Zimmerman Jonathan A
Published: June 2013

The dislocation density tensor at the macroscale may be obtained by using two seemingly disparate definitions given by Nye and Arsenlis and Parks. Nye’s definition depends on counting the dislocations crossing a Burgers circuit of infinitesimal area at the macroscale, where as Arsenlis and Parks’s definition is defined as an integrated property of dislocations within an infinitesimal volume. In this paper, it is shown that Arsenlis and Parks’ and Nye’s definitions for the dislocation density tensor are equivalent when conditions on the length scales of the spacing and curvature of the dislocation lines are obeyed. It is also shown that the definition by Arsenlis and Parks, which can be easily employed in microscopic dislocation dynamics simulations, follows the fundamental extensive property of the Burgers vector, namely, the total Burgers vector of a Burgers circuit is the sum of Burgers vectors of individual dislocation lines intersecting the circuit.
Reconsidering American Indian historical trauma: Lessons from an early Gros Ventre war narrative
Authors: Gone Joseph P.
Published: June 2013

Professional clinicians and human services providers are increasingly attributing the mental health problems of American Indians (AIs) to historical trauma (HT). As an alternative to established psychiatric disorders, AI HT was formulated to explain enduring mental health disparities as originating in tribal experiences of Euro-American colonization. As a result, AI HT has been described as the collective, cumulative, and intergenerational psychosocial disability resulting from massive group-based oppression, such as forced relocation, political subjugation, cultural domination, and genocide. One objective of the HT construct is to frame AI distress and dysfunction in social and historical terms. Given widespread indigenous experiences of colonization, the debilitating effects of HT are presumed to affect most AI communities today. With this background in mind, I explore AI HT with specific reference to a “war narrative” obtained by an anthropologist in 1901 from an elderly Gros Ventre woman. In this account, Watches All described her participation in a historic intertribal battle, and her subsequent captivity and escape from the enemy during the late 1860s. This historical narrative references many first-hand experiences that would today be identified as traumatogenic. Interestingly, however, this account complicates several assumptions underlying AI HT, leading to vexing questions of whether Watches All’s ordeal actually qualifies as an instance of AI HT. No matter how one answers these questions, such ambiguity highlights serious theoretical confusions requiring elaboration and refinement if AI HT is to remain a useful construct in the behavioral health sciences.
Ankle fractures and dislocations: A pictorial review
Authors: Richards PJ , Charran AK , Singhal R , McBride DJ
Published: June 2013

Ankle fractures and dislocations are common injuries. The aim of this pictorial review is to illustrate their radiological assessment, the normal anatomy, the radiographic techniques and the classification of fractures and dislocations in and around the ankle. The mechanism of injury will also be described, which not only can help in classifying the injury, but may also aid in deciding their management. This is not a comprehensive surgical review; however, the importance of determining whether fractures are stable or unstable and the various treatment options are discussed .
The components of the angiotensin-(1-7) system are differentially expressed during follicular wave in cattle
Authors: Barreta Marcos Henrique , Gasperin Bernardo Garziera , Ferreira Rogério , Rovani Monique , Pereira Gabriel Ribas , Bohrer Rodrigo C , de Oliveira João Francisco , Gonçalves Paulo Bayard Dias
Published: June 2013

Introduction:

This study was based on the hypothesis that some components of the angiotensin-(1-7) (Ang-(1-7)) system are differentially expressed during follicular development and can be involved in the follicular health/atresia transition in bovine.

Material and methods:

The largest (F1) and second largest follicles (F2) were collected from cows before (Day 2), during (Day 3), or after (Day 4) the expected moment of follicular deviation. In the second experiment, F1 was induced to atresia through intrafollicular injection of fulvestrant (estrogen receptor-antagonist) and, in both experiments, mRNA expression of the Mas receptor, ACE2, NEP, and PEP was evaluated in the granulosa and theca cells.

Results:

The mRNA expression of Mas receptor was upregulated in the granulosa cells of F2 after the establishment of follicular deviation, while PEP mRNA increased during and after the deviation process. The mRNA expression of ACE2 was upregulated in the granulosa cells of F1 during and after the follicular deviation. The mRNA expression of NEP was not regulated in F1 and F2. Mas receptor expression increased in the F1 induced to atresia.

Conclusions:

mRNA for Mas receptor, ACE2, and PEP are differentially expressed in granulosa cells throughout follicular development and the Mas receptor can be involved with the establishment of follicular dominance.
In vitro comparison of bradykinin degradation by aliskiren, a renin inhibitor, and an inhibitor of angiotensin-converting enzyme
Authors: Joseph Kusumam , Tholanikunnel Tracy E , Kaplan Allen P
Published: June 2013

Introduction:

Angiotensin-converting enzyme (ACE) inhibitors cause angioedema due to diminished degradation of bradykinin. Angiotensin receptor blockers may occasionally cause angioedema but the mechanism is unknown, and are generally considered safe, even in those who have reacted to ACE inhibitors. We determined whether aliskiren, a renin inhibitor, has an effect on the rate of bradykinin degradation.

Methods:

The ability of renin to metabolize bradykinin was studied and the rate of bradykinin degradation compared in the presence or absence of aliskiren. Enalapril, a known ACE inhibitor that causes angioedema served as positive control.

Results:

Renin was unable to digest bradykinin, indicating that a renin inhibitor is unlikely to affect the rate of bradykinin degradation. In a plasma system, aliskiren had no effect on the rate of bradykinin degradation while enalapril inhibited it appreciably. An inhibitory effect of aliskiren on the rate of bradykinin degradation by human pulmonary endothelial cells was observed, estimated to be about 5% of that of enalapril.

Conclusion:

Aliskiren has no effect upon the rate of bradykinin degradation in plasma and a minimal effect employing vascular endothelial cells. The latter suggests inhibition of a non-renin enzyme that is a minor contributor to bradykinin degradation.
Classification of indoor daylight enhancement systems
Authors: Nair MG , Ramamurthy K , Ganesan AR
Published: June 2013

In a dense urban environment, sky obstruction caused by high rise buildings causes minimal daylighting for interiors on lower floors and in deep interior spaces. Innovative daylighting systems can be used to enhance daylighting indoors using available sunlight. This paper presents an overview of recent developments of innovative daylighting systems. The daylighting systems, which are classified as light guiding, light transport, light diffracting, hybrid and integrated systems, have been reviewed and a comparison has been made with respect to ease of integration, durability, maintenance, availability, efficiency, light output and transportation. Suitability of climate has been identified with respect to each daylighting system. Recent research and development involves hybrid and integrated systems which have the advantage of providing lighting irrespective of external sky conditions. The advantages and disadvantages of each system are summarized. Compared to active systems, passive systems are environmentally friendly and their efficiencies are also higher. It is inferred that each system has its own unique principle of light transmittance and is suitable for certain climates. More research is required on developing daylighting systems which are environment friendly, less energy intensive, cost-effective and having ease of integration.
Risk Factors and Gene Type for Infections of MRSA in Diabetic Foot Patients in Tianjin, China
Authors: Feng Shu-Hong , Chu Yue-Jie , Wang Peng-Hua , Jun Xu , Min Ding , Li Xue-Mei
Published: June 2013

The objective was to study risk factors and gene type of DF patients infected with MRSA. A total of 429 DF patients were recruited. The patients with S aureus infections were divided into MRSA and MSSA groups. MRSA were genotyped by SCCmec. pvl and lukE-lukD were detected. A total of 559 pathogens were isolated from them, with G+ bacteria firstly(59.0%), followed G- bacilli (37.7%) and true fungi (3.3%). The 3 most frequently isolated pathogens were S aureus (35.2%), S epidermidis (12.3%), and Pseudomonas aeruginosa (11.2%). SCCmec III MRSA and SCCmec IVa MRSA had the same antibacterial spectrum. mecA positive rate was 100%. lukE-lukD and pvl positive rates were 100% and 0%, respectively. 28 strains belonged to SCCmec III and the others belonged to SCCmec IVa. The G+ cocci were the main pathogens, S aureus and S epidermidis were predominant among them. Antibiotic usage in 6 months prior to hospitalization, long course of ulcer, osteomyelitis and hypoproteinemia are risk factors for MRSA. SCCmec IVa is high in proportion to MRSA isolates, suggesting that CA-MRSA has become major pathogen of DF infection. All the MRSA were harboring lukE-lukD, which has been reported to present poor leucotoxin compared to pvl, and may be a response to atypical local inflammatory reaction in DF infection.
Effect of Daptomycin on Local Interleukin-6, Matrix Metalloproteinase-9, and Metallopeptidase Inhibitor 1 in Patients With MRSA-Infected Diabetic Foot
Authors: Ambrosch Andreas , Halevy Daniel , Fwity Boushra , Brin Thomas , Lobmann Ralf
Published: June 2013

Infection is a major cause of the diabetic foot syndrome being aggravating by the increased burden of multiresistant germs like methicillin-resistant Staphylococcus aureus (MRSA). Maximizing positive outcome for serious MRSA infections requires an aggressive treatment approach and a careful monitoring of the healing process. Therefore, we examined 8 patients with MRSA-infected diabetic foot syndrome Wagner classification grades 2 or 3 (corresponding to the Texas classification stage 2 and 3) during antibiotic treatment with daptomycin. We documented the wound size and obtained samples of wound secretion for analyses of pro-inflammatory interleukin-6 (IL-6), protease (matrix metalloproteinase-9 [MMP-9]), and antiprotease activity (metallopeptidase inhibitor 1 [TIMP-1]). During the course of anti-MRSA therapy, a decrease in the concentration of local IL-6 within the first 3 days followed by a drop of MMP-9 and an increase of TIMP-1 was observed. Finally, a reduction of wound size could be documented. The present data show that efficient antimicrobial treatment with daptomycin leads to a number of beneficial processes at the molecular level of wound healing in MRSA-infected diabetic foot ulcers.
Exercise training improves endothelial function in young prehypertensives
Authors: Beck Darren T , Casey Darren P , Martin Jeffrey S , Emerson Blaze D , Braith Randy W
Published: June 2013

Prehypertensives exhibit marked endothelial dysfunction, a risk factor for future cardiovascular morbidity and mortality. However, the ability of exercise to ameliorate endothelial dysfunction in prehypertensives is grossly underinvestigated. This prospective randomized and controlled study examined the separate effects of resistance and endurance training on conduit artery endothelial function in young prehypertensives. Forty-three unmedicated prehypertensive (systolic blood pressure [SBP]=120–139 mmHg; diastolic blood pressure [DBP]=80–89 mmHg) but otherwise healthy men and women and 15 normotensive matched time-controls (NMTC); n = 15) between 18 and 35 y of age met screening requirements and participated in the study. Prehypertensive subjects were randomly assigned to either a resistance exercise training (PHRT; n = 15), endurance exercise training (PHET; n = 13) or time-control group (PHTC; n = 15). The treatment groups performed exercise training three days per week for eight weeks. The control groups did not initiate exercise programs throughout the study. Flow mediated dilation (FMD) of the brachial artery, biomarkers of enodothelial function and peripheral blood pressure were evaluated before and after exercise intervention or time-matched control. PHRT and PHET reduced resting SBP (9.6 ± 3.6 and 11.9 ± 3.4 mmHg, respectively; P < 0.05) and DBP (8.0 ± 5.1 and 7.2 ± 3.4 mmHg, respectively; P < 0.05). Exercise training improved brachial artery FMD absolute diameter, percent dilation and normalized percent dilation by 30%, 34% and 19% for PHRT, P < 0.05; and by 54%, 63% and 75% for PHET, P < 0.05; respectively. PHRT and PHET increased plasma concentrations of 6-keto prostaglandin F1 α (19% and 22%, respectively; P < 0.05), NO x (19% and 23%, respectively; P < 0.05), and reduced endothelin-1 by (16% and 24%, respectively; P < 0.01). This study provides novel evidence that resistance and endurance exercise separately have beneficial effects on resting peripheral blood pressure, brachial artery FMD and endothelial-derived vasoactive agents in young prehypertensives.
The Cry4B toxin of Bacillus thuringiensis subsp. israelensis kills Permethrin-resistant Anopheles gambiae, the principal vector of malaria
Authors: Ibrahim Mohamed A , Griko Natalya B , Bulla Lee A
Published: June 2013

Resurgence of malaria has been attributed, in part, to the development of resistance by Anopheles gambiae, a principal vector of the disease, to various insecticidal compounds such as Permethrin. Permethrin, a neurotoxicant, is widely used to impregnate mosquito nets. An alternative strategy to control mosquitoes is the use of Bacillus thuringiensis subsp. israelensis (Bti) because there is no observable resistance in the field to the bacterium. Bti kills mosquitoes by targeting cadherin molecules residing in the midgut epithelium of larvae of the insect. Cry proteins (Cry4A, Cry4B, Cry10A and Cry11A) produced by the bacterium during the sporulation phase of its life cycle bind to the cadherin molecules, which serve as receptors for the proteins. These Cry proteins have variable specificity to a variety of mosquitoes, including Culex and Aedes as well as Anopheles. Importantly, selective mosquitocidal action is occasioned by binding of the respective Cry toxins to cadherins distinctive to individual mosquito species. Differential fractionation of the four Cry proteins from a novel Bti isolate (M1) and cloning and expression of their genes in Escherichia coli revealed that Cry4B is the only Cry protein that exerts insecticidal action against An. gambiae. Indeed, it does so against a Permethrin-resistant strain of the mosquito. The other three Cry proteins are ineffective. Multiple sequence alignments of the four Cry proteins revealed a divergent sequence motif in the Cry4B toxin, which most likely determines binding of the toxin to its cognate receptor, BT-R3, in An. gambiae and to its specific toxicity. A model showing Cry4B toxin binding to BT-R3 is presented.
Interleukin-21 up-regulates interleukin-21R expression and interferon gamma production by CD8+ cells in SHIV-infected macaques
Authors: Zhao Chang-cheng , Gao Xi-qiang , Xue Jing , Cong Zhe , Zhang Wei-lun , Chen Ting , Wu Fang-xin , Xiong Jing , Ju Bin , Su Aihua , Wei Qiang , Qin Chuan
Published: June 2013

Interleukin-21 (IL-21) is produced primarily by CD4+ T cells and regulates immunity against human/simian immunodeficiency virus (HIV/SIV) infection. Activated CD8+ cells and their secreted interferon-gamma (IFN- γ) are crucial for the control of acute HIV/SIV infection. However, whether IL-21 can regulate IFN- γ production by CD8+ cells remains controversial. Rhesus macaques (RMs, n = 8) were infected with SHIV and the levels of plasma IL-21, IFN- γ and the frequency of peripheral blood activated T cells were measured longitudinally. Following infection with SHIV, the levels of plasma IL-21 and IFN- γ increased, peaked at 17 days postinfection and declined later. Furthermore, IL-21 induced IL-21 receptor (IL-21R) and IFN- γ, perforin, but not granmyze B, expression in CD8+ cells from four selected SHIV-infected RMs. The regulatory effect of IL-21 on CD8+ cell function appeared to be associated with increased levels of STAT3, but not STAT5, phosphorylation in CD8+ cells from SHIV-infected RMs. In parallel, treatment with soluble IL-21R/Fc, an inhibitor of IL-21-induced activation of JAK1/3 and STAT3, abrogated IL-21-induced STAT3 activation and IFN- γ production in CD8+ cells from SHIV-infected RMs in vitro. Our data indicated that IL-21 was a positive regulator of IFN- γ-secreting CD8+ cells and increased the STAT3 phosphorylation, regulating T-cell immunity against acute SHIV infection in RMs. Our findings may provide a new basis for the development of immunotherapies for the control of SHIV/HIV infection.
Acute glutathione depletion induces hepatic methylglyoxal accumulation by impairing its detoxification to d-lactate
Authors: Masterjohn Christopher , Mah Eunice , Park Youngki , Pei Ruisong , Lee Jiyoung , Manautou Jose E , Bruno Richard S
Published: June 2013

Methylglyoxal (MGO) is a dicarbonyl that reacts with amino acids and nucleic acids to form advanced glycation endproducts, which may contribute to diabetes and its cardiovascular complications. MGO detoxification through the glyoxalase (GLO) pathway is glutathione (GSH)-dependent, but no studies have investigated whether acute depletion of GSH regulates MGO accumulation in vivo. We therefore administered a single intraperitoneal injection of the specific GSH biosynthesis inhibitor l-buthionine-( RS)-sulfoximine (BSO; 4 mmol/kg) or phosphate-buffered saline vehicle to six-week-old Sprague Dawley rats ( n = 48) prior to sacrificing at 0, 6, 12 and 48 h ( n = 6/time point/treatment). BSO had no effect ( P > 0.05) on adipose or plasma MGO at any specific time points following treatment. In contrast, hepatic GSH was 68–71% lower ( P < 0.05) at 6–12 h following BSO, and MGO was 27% higher at 12 h. At 12 h, hepatic d-lactate was 13% lower and GLO activity was 52% lower following BSO, which was fully restored by the exogenous addition of GSH. Hepatic GSH was inversely related to hepatic MGO ( r = −0.81; P < 0.01) and positively correlated with hepatic GLO activity ( r = 0.72; P < 0.01), whereas hepatic GLO activity was positively correlated with hepatic d-lactate ( r = 0.63; P < 0.05). BSO had no effect on hepatic malondialdehyde or vitamin E. These findings demonstrate that GSH depletion in vivo increases hepatic MGO accumulation by impairing its GSH-dependent, GLO-mediated detoxification to d-lactate independent of oxidative stress.
Methionine restriction affects oxidative stress and glutathione-related redox pathways in the rat
Authors: Maddineni Sreenivasa , Nichenametla Sailendra , Sinha Raghu , Wilson Ronald P , Richie John P
Published: June 2013

Lifelong dietary methionine restriction (MR) is associated with increased longevity and decreased incidence of age-related disorders and diseases in rats and mice. A reduction in the levels of oxidative stress may be a contributing mechanistic factor for the beneficial effects of MR. To examine this, we determined the effects of an 80% dietary restriction of Met on different biomarkers of oxidative stress and antioxidant pathways in blood, liver, kidney and brain in the rat. Male F-344 rats were fed control (0.86% methionine) or MR (0.17% methionine) diets for up to six months. Blood and tissues were analyzed for glutathione (GSH) concentrations, related enzyme activities and biomarkers of oxidative stress. MR was associated with reductions in oxidative stress biomarkers including plasma 8-hydoxydeoxyguanosine (8-OHdG) and 8-isoprostane and erythrocyte protein-bound glutathione after one month with levels remaining low for at least six months ( P < 0.05). Levels of free GSH in blood were increased after 1–6 months of MR feeding whereas liver GSH levels were reduced over this time ( P < 0.05). In MR rats, GSH peroxidase activity was decreased in liver and increased in kidney compared with controls. No changes in the activities of GSH reductase in liver and kidney and superoxide dismutase in liver were observed as a result of MR feeding. Altogether, these findings indicate that oxidative stress is reduced by MR feeding in rats, but this effect cannot be explained by changes in the activity of antioxidant enzymes.
Rehabilitating Walking Speed Poststroke With Treadmill-Based Interventions
Authors: Charalambous Charalambos C. , Bonilha Heather Shaw , Kautz Steven A. , Gregory Chris M. , Bowden Mark G.
Published: June 2013

Background. In the past several years, several randomized controlled trials (RCTs) have been reported regarding the efficacy of treadmill-based walking-specific rehabilitation programs, either individually (TT) or combined with body weight support (BWSTT), over control group therapies poststroke. No clear consensus exists as to whether treadmill-based interventions are superior in rehabilitating walking speed (WS) poststroke. Objective. To review published RCTs examining TT and BWSTT poststroke and describe the effects on improving and retaining WS. Methods. A systematic literature search in computerized databases was conducted to identify RCTs whose methodological quality was assessed with PEDro. Pre- and post-WS, change in WS, functional outcomes, and follow-up speed were extracted and calculated from each study. Additionally, statistical results of each study were examined, and the intragroup and intergroup effect sizes (ESintra and ESinter, respectively) were calculated. Results. All studies (8 TT; 7 BWSTT) met the inclusion criteria, and their methodological quality was generally good, with a mean PEDro score 6.9/10. Of the 15 studies, 8 studies (4 TT; 4 BWSTT) reported intragroup significant increases of WS, whereas only 4 (4 TT) found superiority of treadmill interventions. Nine studies demonstrated large ESintra (4 TT; 5 BWSTT), yet only 3 showed large ESinter (1 TT; 2 BWSTT). Four studies (2 TT and 2 BWSTT) reported retention of gains in WS, regardless of intervention. Conclusions. Treadmill-based interventions poststroke may increase and retain WS, but their universal superiority to other control group therapies has failed to be established.
Responsiveness of Upper Extremity Kinematic Measures and Clinical Improvement During the First Three Months After Stroke
Authors: Alt Murphy Margit , Willén Carin , Sunnerhagen Katharina S.
Published: June 2013

Background. Kinematic movement analysis is increasingly used as an outcome measure in evaluation of upper extremity function after stroke. Little is known, however, about what observed longitudinal changes in kinematics mean in the context of an individual’s functioning. In this study, the responsiveness and expected change in kinematic measures associated with clinically meaningful improvement in the upper extremity were evaluated. Methods. Kinematic movement analysis of a drinking task and Action Research Arm Test (ARAT) were performed early (9 days poststroke) and at 3 months after stroke in 51 subjects. The receiver-operating characteristic curve and linear regression analyses were used to evaluate responsiveness of kinematic parameters. Results. Movement time, smoothness, and trunk displacement discriminated those subjects demonstrating clinically meaningful improvements. Significant associations of 31% to 36% were found between the change in ARAT and kinematic measures. A real clinical improvement in kinematics lies in the range of 2.5 to 5 seconds, 3 to 7 units, and 2 to 5 cm in movement time, smoothness, and trunk displacement, respectively. Conclusions. All kinematic measures reported in this study are responsive measures for capturing improvements in the upper extremity during the first 3 months after stroke. Approximate estimates for the expected change in kinematics associated with clinically meaningful improvement in upper extremity activity capacity illustrate the usefulness of the linear regression analysis for assessing responsiveness. This knowledge facilitates the selection of kinematic measures for clinical and movement analysis research as well as for technology-based devices.
KinesioTaping Reduces Pain and Modulates Sensory Function in Patients With Focal Dystonia
Authors: Pelosin Elisa , Avanzino Laura , Marchese Roberta , Stramesi Paola , Bilanci Martina , Trompetto Carlo , Abbruzzese Giovanni
Published: June 2013

Background. Pain is one of the most common and disabling “nonmotor” symptoms in patients with dystonia. No recent study evaluated the pharmacological or physical therapy approaches to specifically treat dystonic pain symptoms. Objective. To evaluate the effectiveness of KinesioTaping in patients with cervical dystonia (CD) and focal hand dystonia (FHD) on self-reported pain (primary objective) and on sensory functions (secondary objective). Methods. Twenty-five dystonic patients (14 with CD and 11 FHD) entered a randomized crossover pilot study. The patients were randomized to 14-day treatment with KinesioTaping or ShamTaping over neck (in CD) or forearm muscles (in FHD), and after a 30-day washout period, they received the other treatment. The main outcome measures were 3 visual analog scales (VASs) for usual pain, worst pain, and pain relief. Disease severity changes were evaluated by means of the Toronto Western Spasmodic Torticollis Rating Scale (CD) and the Writer’s Cramp Rating Scale (FHD). Furthermore, to investigate possible KinesioTaping-induced effects on sensory functions, we evaluated the somatosensory temporal discrimination threshold. Results. Treatment with KinesioTape induced a decrease in the subjective sensation of pain and a modification in the ability of sensory discrimination, whereas ShamTaping had no effect. A significant, positive correlation was found in both groups of patients between the improvement in the subjective sensation of pain and the reduction of somatosensory temporal discrimination threshold values induced by KinesioTaping. Conclusions. These preliminary results suggest that KinesioTaping may be useful in treating pain in patients with dystonia.
Lessons learned from a resident-led clinical trial in obstetrics
Authors: Costescu Dustin JW , Cullimore Amie J
Published: June 2013

Background

Completion of a randomised controlled trial is one way by which the resident research requirement can be met in Canadian obstetrics and gynaecology programmes. However, little is known about the specific challenges of performing clinical trials within the specialty, let alone as a resident project.

Purpose

A resident-led randomised controlled trial comparing two methods of labour induction at term was halted due to insufficient patient enrolment. A structured review of the study design and recruitment process was conducted to identify factors contributing to poor recruitment.

Methods

In addition to completing a literature review and internal review by the research team, we surveyed obstetricians and residents regarding recruitment efforts and barriers to participation. We solicited feedback on trial design and the expectations of clinicians with respect to participation in research studies.

Results

Eight obstetricians (67%) and 13 residents (93%) responded to the survey. All were able to identify eligible patients, though only 60% had invited one or more patients to participate during the recruitment period. Failure to consider trial participation and excessive clinical workload were the most commonly cited barriers for clinicians. Resistance to the test intervention was the major barrier to patient participation. Several residents cited a lack of personal incentive to recruit patients as a significant barrier.

Limitations

The research team was unable to contact patients directly, thus limiting the scope of our review to our internal methods and feedback from clinicians.

Conclusions

Poor recruitment in a resident-led clinical trial in obstetrics resulted from multiple coexisting factors. A structured review provided valuable insight for the research team. Academic clinicians and trainees in all specialties should be encouraged to share their experiences in the hope of improving the likelihood of success in future research endeavours.
Clinical biobanks in Italy and Liguria: Ethical and social issues, initiatives at the national, regional and local level
Authors: Parodi Barbara
Published: June 2013

This article aims to revise the ethical and social implications for clinical biobanks and their application in Italy, in the Liguria Region and in a comprehensive cancer centre in Genoa. The policies already in place in the regional network and in the IST National Institute for Cancer Research in terms of involvement of the community of patients and citizens are described, as well as the future development of initiatives aimed at improving the active participation of the community. The author is a biobank manager and not a professional bioethicist. The world of biobanks in Italy includes large numbers of small initiatives, and ‘biobankers’ are aware that public engagement is essential to ensure public trust and to give legitimacy to the research. The governance of these research infrastructures requires new tools in order to improve involvement of participants. The October 2011 Conference, ‘New Patient-Centric Perspectives in Medical Research: Ethical and Governance Challenges’ has been an important occasion for bringing together different experiences and expertise and for learning about new forms of participant-centric approaches and tools.
Public deliberation to develop ethical norms and inform policy for biobanks: Lessons learnt and challenges remaining
Authors: O’Doherty Kieran C , Burgess Michael M
Published: June 2013

Public participation is increasingly an aspect of policy development in many areas, and the governance of biomedical research is no exception. There are good reasons for this: biomedical research relies on public funding; it relies on biological samples and information from large numbers of patients and healthy individuals; and the outcomes of biomedical research are dramatically and irrevocably changing our society. There is thus arguably a democratic imperative for including public values in strategic decisions about the governance of biomedical research. However, it is not immediately clear how this might best be achieved. While different approaches have been proposed and trialled, we focus here on the use of public deliberation as a mechanism to develop input for policy on biomedical research. We begin by explaining the rationale for conducting public deliberation in biomedical research. We focus, in particular, on the ELS (ethical, legal, social) aspects of human tissue biobanking. The last few years have seen the development of methods for conducting public deliberation on these issues in several jurisdictions, for the purpose of incorporating lay public voices in biobanking policy. We explain the theoretical foundation underlying the notion of deliberation, and outline the main lessons and capacities that have been developed in the area of conducting public deliberation on biobanks. We next provide an analysis of the theoretical and practical challenges that we feel still need to be addressed for the use of public deliberation to guide ethical norms and governance of biomedical research. We examine the issues of: (i) linking the outcomes of deliberation to tangible action; (ii) the mandate under which a deliberation is conducted; (iii) the relative weight that should be accorded to a public deliberative forum vs other relevant voices; (iv) evaluating the quality of deliberation; and (5) the problem of scalability of minipublics.
The cognitive side of essential tremor: what are the therapeutic implications?
Authors: Janicki Sarah C. , Cosentino Stephanie , Louis Elan D.
Published: June 2013

While essential tremor (ET) has traditionally been categorized as a pure motor disease, cross-sectional and longitudinal studies of cognition in ET have demonstrated that these patients may have cognitive dysfunction. Recent epidemiological studies demonstrate an association between ET (particularly with onset after age 65) and increased risk for cognitive impairment and dementia. Although existing studies have generally conceptualized cognitive changes in ET as consistent with a ‘frontosubcortical’ or ‘corticocerebellar’ profile, results from these same studies suggest that cognitive impairment in ET may in fact be heterogeneous. Furthermore, the underlying mechanisms remain uncertain. Cognitive changes could be a byproduct of the cerebellar dysfunction of ET itself; alternately, they may be a feature of concomitant neurodegenerative diseases that have been associated in several studies with ET, including Alzheimer’s disease, Parkinson’s disease or progressive supranuclear palsy. While the study of cognitive dysfunction in ET has received research attention in recent years, the results of these studies have not been translated into the clinical domain and clinical practice. This review first summarizes the current literature on the potential relationships between ET and cognitive change. We then suggest areas of further clinical evaluation and treatment; these suggestions are directed at physicians caring for ET patients who may demonstrate or complain of cognitive impairment. As we discuss, clinicians should ideally screen ET patients for possible signs or symptoms of cognitive impairment in addition to assessing for psychiatric comorbidity and quality of life. These recommendations are in contrast to most current clinical practice, which does not routinely include such assessment among ET patients. To our knowledge, there have been no pharmacotherapeutic trials to date of any agent for cognitive change associated with ET. We believe that studies for this indication are now called for. Future efforts in this direction will also need to take into account the pathobiology of cognitive changes in ET, which itself is an area that is ripe for future investigations.
Social Rejection Biases Estimates of Interpersonal Distance
Authors: Knowles Megan L. , Green Allison , Weidel Alicia
Published: June 2013

Given the power of belonging needs to shape individuals’ thoughts, feelings, and behavior, we posited that people’s desire for reconnection even influences judgments of physical distance. We hypothesized that rejection motivates individuals to distance themselves from sources of rejection and draw near those who are accepting. We tested this hypothesis in five studies. Participants recalled someone who had rejected or accepted them previously (Study 1), tossed a ball with inclusive and exclusive confederates (Study 2), and relived a past rejection, acceptance, or failure in the presence of an uninvolved other (Studies 3–5). Participants provided retrospective estimates of distance to rejecting and accepting others (Studies 1–2) and to uninvolved others (Studies 3–5). Participants reported that (1) accepting others were closer than rejecting others and (2) uninvolved others were closer than nonsocial targets after rejection but not acceptance or failure. Findings suggest that individuals distort perceptions of distance to serve belonging needs.