bluuebunny/crossref_metadata_2025 huggingface dataset:
Hua-Tan-Sheng-Jing Decoction Treats Obesity With Oligoasthenozoospermia by Up-Regulating the PI3K-AKT and Down-Regulating the JNK MAPK Signaling Pathways: At the Crossroad of Obesity and Oligoasthenozoospermia
Authors: Dong Yang , Zheng Yanfei , Zhu Linghui , Li Tianxing , Guan Yuanyuan , Zhao Shipeng , Wang Qi , Wang Ji , Li Lingru
Published: April 2022

Background: Oligoasthenozoospermia is the leading cause of male infertility, seriously affecting men’s health and increasing the societal medical burden. In recent years, obesity-related oligoasthenozoospermia has attracted increased attention from researchers to find a cure. This study aimed to evaluate the efficacy of Hua-Tan-Sheng-Jing decoction (HTSJD) in treating obesity with oligoasthenozoospermia, determine its active ingredients and identify its mechanism of action.

Methods: The ingredients of HTSJD were determined by combining the ultra-performance liquid chromatography with mass spectrometry (UPLC-MS/MS) and systems pharmacology approach. The common pathogenesis of obesity and oligoasthenozoospermia and the potential mechanism of HTSJD against obesity with oligoasthenozoospermia were obtained through target fishing, network construction, and enrichment analyses. Further, molecular docking of the key ingredients with the upstream receptors of the key signaling pathways of the potential mechanism was used to predict their affinity. Finally, high-fat-induced obesity with oligoasthenozoospermia rat model was constructed to determine the effects of HTSJD on semen concentration, sperm motility, body weight, and serum lipid metabolism. The key proteins were validated by immunohistochemistry (IHC).

Results: A total of 70 effective components and 847 potential targets of HTSJD (H targets) were identified, of which 743 were common targets related to obesity and oligoasthenozoospermia (O-O targets) mainly enriched in the pathways related to inflammation, oxidative stress and hormone regulation. Finally, 143 common targets (H-O-O targets) for HTSJD against obesity with oligoasthenozoospermia were obtained. Combining the hub genes and the results of Gene Ontology (GO) functional and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of H-O-O targets, PI3K-AKT and MAPK signaling pathways were identified as the key pathways. Molecular docking results showed that Diosgenin, Kaempferol, Quercetin, Hederagenin, Isorhamnetin may act on the related pathways by docking EGFR, IGF1R and INSR. The animal-based in vivo experiments confirmed that HTSJD improves the sperm quality of high-fat diet-fed rats by reducing their body weight and blood lipid levels, influencing the PI3K-AKT and MAPK signaling pathways and altering the corresponding protein expressions.

Conclusion: HTSJD treats obesity with oligoasthenozoospermia by up-regulating the PI3K-AKT signaling pathway and down-regulating the MAPK signaling pathway, which are at the crossroad of obesity and oligoasthenozoospermia.
Plasma-Surface Interactions Within Helicon Plasma Sources
Authors: Del Valle Juan I. , Chang Diaz Franklin R. , Granados Víctor H.
Published: April 2022

Helicon plasma sources do not require electrodes or grids directly immersed in the plasma, and also present an axial magnetic field confining the plasma discharge. These factors are believed to provide them with long operational lifetimes because of the reduced potential for surface etching. The physics of helicon waves, cylindrical magnetized plasmas, sheaths, and plasma-surface interactions are discussed in the context of this claim. Practical implementation aspects are also reviewed, along with relevant experimental results. It is shown that understanding the distribution of ion density within the source, the presence of induced potentials in its surfaces, and the physics of low-energy sputtering reactions is essential to properly model erosion phenomena within helicons, and consequently predict their performance in practical applications.
High-Dimensional Entanglement of Photonic Angular Qudits
Authors: Puentes Graciana
Published: April 2022

We propose a method for generation of entangled photonic states in high dimensions, the so-called qudits, by exploiting quantum correlations of Orbital Angular Momentum (OAM) entangled photons, produced via Spontaneous Parametric Down Conversion. Diffraction masks containing N angular slits placed in the path of twin photons define a qudit space of dimension N2, spanned by the alternative pathways of OAM-entangled photons. We quantify the high-dimensional entanglement of path-entangled photons by the Concurrence, using an analytic expression valid for pure states. We report numerical results for the Concurrence as a function of the angular aperture size for the case of high-dimensional OAM entanglement and for the case of high-dimensional path entanglement, produced by N × M angular slits. Our results provide additional means for preparation and characterization of entangled quantum states in high-dimensions, a fundamental resource for quantum simulation and quantum information protocols.
The Physiological Basis of High-Frequency Oscillatory Ventilation and Current Evidence in Adults and Children: A Narrative Review
Authors: Miller Andrew G. , Tan Herng Lee , Smith Brian J. , Rotta Alexandre T. , Lee Jan Hau
Published: April 2022

High-frequency oscillatory ventilation (HFOV) is a type of invasive mechanical ventilation that employs supra-physiologic respiratory rates and low tidal volumes (VT) that approximate the anatomic deadspace. During HFOV, mean airway pressure is set and gas is then displaced towards and away from the patient through a piston. Carbon dioxide (CO2) is cleared based on the power (amplitude) setting and frequency, with lower frequencies resulting in higher VTand CO2clearance. Airway pressure amplitude is significantly attenuated throughout the respiratory system and mechanical strain and stress on the alveoli are theoretically minimized. HFOV has been purported as a form of lung protective ventilation that minimizes volutrauma, atelectrauma, and biotrauma. Following two large randomized controlled trials showing no benefit and harm, respectively, HFOV has largely been abandoned in adults with ARDS. A multi-center clinical trial in children is ongoing. This article aims to review the physiologic rationale for the use of HFOV in patients with acute respiratory failure, summarize relevant bench and animal models, and discuss the potential use of HFOV as a primary and rescue mode in adults and children with severe respiratory failure.
Fluorescent Microscopy-Based Detection of Chitin in Intact Drosophila melanogaster
Authors: Flaven-Pouchon J. , Moussian B.
Published: April 2022

Chitin is the major scaffolding component of the insect cuticle. Ultrastructural analyses revealed that chitin adopts a quasi-crystalline structure building sheets of parallel running microfibrils. These sheets called laminae are stacked either helicoidally or with a preferred orientation of the microfibrils. Precise control of chitin synthesis is mandatory to ensure the correct chitin assembly and in turn proper function of cuticular structures. Thus, evaluation of chitin-metabolism deficient phenotypes is a key to our understanding of the function of the proteins and enzymes involved in cuticle architecture and more generally in cuticle biology in insects. Usually, these phenotypes have been assessed using electron microscopy, which is time-consuming and labor intensive. This stresses the need for rapid and straightforward histological methods to visualize chitin at the whole tissue level. Here, we propose a simple method of chitin staining using the common polysaccharide marker Fluorescent brightener 28 (FB28) in whole-mount Drosophila melanogaster. To overcome the physical barrier of FB28 penetration into the cuticle, staining is performed at 65°C without affecting intactness. We quantify FB28 fluorescence in three functionally different cuticular structures namely wings, dorsal abdomens and forelegs by fluorescence microscopy. We find that, as expected, cuticle pigmentation may interfere with FB28 staining. Down-regulation of critical genes involved in chitin metabolism, including those coding for chitin synthase or chitinases, show that FB28 fluorescence reflects chitin content in these organs. We think that this simple method could be easily applied to a large variety of intact insects.
Deuterated Linoleic Acid Attenuates the RBC Storage Lesion in a Mouse Model of Poor RBC Storage
Authors: Kim Christopher Y. , Johnson Hannah , Peltier Sandy , Spitalnik Steven L. , Hod Eldad A. , Francis Richard O. , Hudson Krystalyn E. , Stone Elizabeth F. , Gordy Dominique E. , Fu Xiaoyun , Zimring James C. , Amireault Pascal , Buehler Paul W. , Wilson Robert B. , D’Alessandro Angelo , Shchepinov Mikhail S. , Thomas Tiffany
Published: April 2022

Background: Long-chain polyunsaturated fatty acids (PUFAs) are important modulators of red blood cell (RBC) rheology. Dietary PUFAs are readily incorporated into the RBC membrane, improving RBC deformability, fluidity, and hydration. However, enriching the lipid membrane with PUFAs increases the potential for peroxidation in oxidative environments (e.g., refrigerated storage), resulting in membrane damage. Substitution of bis-allylic hydrogens with deuterium ions in PUFAs decreases hydrogen abstraction, thereby inhibiting peroxidation. If lipid peroxidation is a causal factor in the RBC storage lesion, incorporation of deuterated linoleic acid (DLA) into the RBC membrane should decrease lipid peroxidation, thereby improving RBC lifespan, deformability, filterability, and post-transfusion recovery (PTR) after cold storage.

Study Design and Methods: Mice associated with good (C57BL/6J) and poor (FVB) RBC storage quality received diets containing 11,11-D2-LA Ethyl Ester (1.0 g/100 g diet; deuterated linoleic acid) or non-deuterated LA Ethyl Ester (control) for 8 weeks. Deformability, filterability, lipidomics, and lipid peroxidation markers were evaluated in fresh and stored RBCs.

Results: DLA was incorporated into RBC membranes in both mouse strains. DLA diet decreased lipid peroxidation (malondialdehyde) by 25.4 and 31% percent in C57 mice and 12.9 and 79.9% in FVB mice before and after cold storage, respectively. In FVB, but not C57 mice, deformability filterability, and post-transfusion recovery were significantly improved.

Discussion: In a mouse model of poor RBC storage, with elevated reactive oxygen species production, DLA attenuated lipid peroxidation and significantly improved RBC storage quality.
Oxygenation Performance of Different Non-Invasive Devices for Treatment of Decompression Illness and Carbon Monoxide Poisoning
Authors: Köhler Andrea , Zoll Felicitas M. , Ploner Thomas , Hammer Alexander , Joannidis Michael , Tilg Herbert , Finkenstedt Armin , Hartig Frank
Published: April 2022

Study Objective:Application of high concentrations of oxygen to increase oxygen partial pressure (pO2) is the most important treatment for patients with carbon monoxide intoxication or divers with suspected decompression illness. The aim of this study was to evaluate the oxygenation performance of various non-invasive oxygen systems.

Methods:The effect of different oxygen systems on arterial pO2, pCO2 and pH and their subjective comfort was evaluated in 30 healthy participants. Eight devices were included: nasal cannula, non-rebreather mask, AirLife Open mask, Flow-Safe II CPAP device, SuperNO2VA nasal PAP device, all operated with 15 L/min constant flow oxygen; nasal high-flow (50 L/min flow, 1.0 FiO2), non-invasive positive pressure ventilation (NPPV, 12 PEEP, 4 ASB, 1.0 FiO2) and a standard diving regulator (operated with pure oxygen).

Results:Diving regulator, SuperNO2VA, nasal high-flow and NPPV achieved mean arterial pO2 concentrations between 538 and 556 mm Hg within 5 minutes. The AirLife Open mask, the nasal cannula and the non-rebreather mask achieved concentrations of 348–451 mm Hg and the Flow-Safe II device 270 mm Hg. Except for the AirLife open mask, pCO2 decreased and pH increased with all devices. The highest pH values were observed with NPPV, diving regulator, Flow-Safe II and nasal high-flow but apparent hyperventilation was uncommon. The AirLife Open and the non-rebreather mask were the most comfortable, the SuperNO2VA and the nasal cannula the most uncomfortable devices.

Conclusion:A standard diving regulator and the SuperNO2VA device were equally effective in providing highest physiologically possible pO2 as compared to nasal high-flow and NPPV.
Two Dominant Herbaceous Species Have Different Plastic Responses to N Addition in a Desert Steppe
Authors: Guo Aixia , Zuo Xiaoan , Hu Ya , Yue Ping , Li Xiangyun , Lv Peng , Zhao Shenglong
Published: April 2022

Nitrogen (N) deposition rates are increasing in the temperate steppe due to human activities. Understanding the plastic responses of plant dominant species to increased N deposition through the lens of multiple traits is crucial for species selection in the process of vegetation restoration. Here, we measured leaf morphological, physiological, and anatomical traits of two dominant species (Stipa glareosa and Peganum harmala) after 3-year N addition (0, 1, 3, and 6 g N m−2 year−1, designated N0, N1, N3, and N6, respectively) in desert steppe of Inner Mongolia. We separately calculated the phenotypic plasticity index (PI) of each trait under different N treatments and the mean phenotypic plasticity index (MPI) of per species. The results showed that N addition increased the leaf N content (LNC) in both species. N6 increased the contents of soluble protein and proline, and decreased the superoxide dismutase (SOD) and the peroxidase (POD) activities of S. glareosa, while increased POD and catalase (CAT) activities of P. harmala. N6 increased the palisade tissue thickness (PT), leaf thickness (LT), and palisade-spongy tissue ratio (PT/ST) and decreased the spongy tissue–leaf thickness ratio (ST/LT) of S. glareosa. Furthermore, we found higher physiological plasticity but lower morphological and anatomical plasticity in both species, with greater anatomical plasticity and MPI in S. glareosa than P. harmala. Overall, multi-traits comparison reveals that two dominant desert-steppe species differ in their plastic responses to N addition. The higher plasticity of S. glareosa provides some insight into why S. glareosa has a broad distribution in a desert steppe.
Alignment-Free Analysis of Whole-Genome Sequences From Symbiodiniaceae Reveals Different Phylogenetic Signals in Distinct Regions
Authors: Lo Rosalyn , Dougan Katherine E. , Chen Yibi , Shah Sarah , Bhattacharya Debashish , Chan Cheong Xin
Published: April 2022

Dinoflagellates of the family Symbiodiniaceae are predominantly essential symbionts of corals and other marine organisms. Recent research reveals extensive genome sequence divergence among Symbiodiniaceae taxa and high phylogenetic diversity hidden behind subtly different cell morphologies. Using an alignment-free phylogenetic approach based on sub-sequences of fixed length k (i.e. k-mers), we assessed the phylogenetic signal among whole-genome sequences from 16 Symbiodiniaceae taxa (including the genera of Symbiodinium, Breviolum, Cladocopium, Durusdinium and Fugacium) and two strains of Polarella glacialis as outgroup. Based on phylogenetic trees inferred from k-mers in distinct genomic regions (i.e. repeat-masked genome sequences, protein-coding sequences, introns and repeats) and in protein sequences, the phylogenetic signal associated with protein-coding DNA and the encoded amino acids is largely consistent with the Symbiodiniaceae phylogeny based on established markers, such as large subunit rRNA. The other genome sequences (introns and repeats) exhibit distinct phylogenetic signals, supporting the expected differential evolutionary pressure acting on these regions. Our analysis of conserved core k-mers revealed the prevalence of conserved k-mers (>95% core 23-mers among all 18 genomes) in annotated repeats and non-genic regions of the genomes. We observed 180 distinct repeat types that are significantly enriched in genomes of the symbiotic versus free-living Symbiodinium taxa, suggesting an enhanced activity of transposable elements linked to the symbiotic lifestyle. We provide evidence that representation of alignment-free phylogenies as dynamic networks enhances the ability to generate new hypotheses about genome evolution in Symbiodiniaceae. These results demonstrate the potential of alignment-free phylogenetic methods as a scalable approach for inferring comprehensive, unbiased whole-genome phylogenies of dinoflagellates and more broadly of microbial eukaryotes.
Tubulin Cytoskeleton Organization in Cells of Determinate Nodules
Authors: Kitaeva Anna B. , Gorshkov Artemii P. , Kusakin Pyotr G. , Sadovskaya Alexandra R. , Tsyganova Anna V. , Tsyganov Viktor E.
Published: April 2022

Plant cell differentiation is based on rearrangements of the tubulin cytoskeleton; this is also true for symbiotic nodules. Nevertheless, although for indeterminate nodules (with a long-lasting meristem) the organization of microtubules during nodule development has been studied for various species, for determinate ones (with limited meristem activity) such studies are rare. Here, we investigated bacteroid morphology and dynamics of the tubulin cytoskeleton in determinate nodules of four legume species: Glycine max, Glycine soja, Phaseolus vulgaris, and Lotus japonicus. The most pronounced differentiation of bacteroids was observed in G. soja nodules. In meristematic cells in incipient nodules of all analyzed species, the organization of both cortical and endoplasmic microtubules was similar to that described for meristematic cells of indeterminate nodules. In young infected cells in developing nodules of all four species, cortical microtubules formed irregular patterns (microtubules were criss-crossed) and endoplasmic ones were associated with infection threads and infection droplets. Surprisingly, in uninfected cells the patterns of cortical microtubules differed in nodules of G. max and G. soja on the one hand, and P. vulgaris and L. japonicus on the other. The first two species exhibited irregular patterns, while the remaining two exhibited regular ones (microtubules were oriented transversely to the longitudinal axis of cell) that are typical for uninfected cells of indeterminate nodules. In contrast to indeterminate nodules, in mature determinate nodules of all four studied species, cortical microtubules formed a regular pattern in infected cells. Thus, our analysis revealed common patterns of tubulin cytoskeleton in the determinate nodules of four legume species, and species-specific differences were associated with the organization of cortical microtubules in uninfected cells. When compared with indeterminate nodules, the most pronounced differences were associated with the organization of cortical microtubules in nitrogen-fixing infected cells. The revealed differences indicated a possible transition during evolution of infected cells from anisotropic growth in determinate nodules to isodiametric growth in indeterminate nodules. It can be assumed that this transition provided an evolutionary advantage to those legume species with indeterminate nodules, enabling them to host symbiosomes in their infected cells more efficiently.
Mining Beneficial Genes for Salt Tolerance From a Core Collection of Rice Landraces at the Seedling Stage Through Genome-Wide Association Mapping
Authors: Wang Xiaoliang , Li Jinquan , Sun Jian , Gu Shuang , Wang Jingbo , Su Chang , Li Yueting , Ma Dianrong , Zhao Minghui , Chen Wenfu
Published: April 2022

Rice is a salt-sensitive plant. High concentration of salt will hinder the absorption of water and nutrients and ultimately affect the yield. In this study, eight seedling-stage salt-related traits within a core collection of rice landraces were evaluated under salinity stress (100 mM NaCl) and normal conditions in a growth chamber. Genome-wide association study (GWAS) was performed with the genotypic data including 2,487,353 single-nucleotide polymorphisms (SNPs) detected in the core collection. A total of 65 QTLs significantly associated with salt tolerance (ST) were identified by GWAS. Among them, a co-localization QTL qTL4 associated with the SKC, RN/K, and SNC on chromosome 6, which explained 14.38–17.94% of phenotypic variation, was selected for further analysis. According to haplotype analysis, qRT-PCR analysis, and sequence alignment, it was finally determined that 4 candidate genes (LOC_Os06g47720, LOC_Os06g47820, LOC_Os06g47850, LOC_Os06g47970) were related to ST. The results provide useful candidate genes for marker assisted selection for ST in the rice molecular breeding programs.
A Case Study of the Relationship Between Vegetation Coverage and Urban Heat Island in a Coastal City by Applying Digital Twins
Authors: Qi Yansu , Li Han , Pang Zonglin , Gao Weijun , Liu Chao
Published: April 2022

While urban vegetation affects the urban thermal environment directly, the effects of different plant layouts and vegetation cover on urban microclimate regulation are different. This study has applied digital technologies to advance urban environmental research and forestry analysis. With a focus on a coastal city located on the eastern coast of the North Temperate Zone as a study area, this study collected the Landsat archive satellite remote sensing image data covering the study area in 2000–2020 and analyzed the temporal and spatial distribution characteristics of vegetation coverage, land surface temperature, and urban heat island (UHI) ratio index. The study results included the following findings: (1) The area of high fractional vegetation cover (FVC) (0.8–1.0) in the study area is increasing. Those areas are located in the mountain forests in the near-coastal area. The lowest temperature was also detected in the mountain area. (2) The distance from the coastline causes a negative correlation between land surface temperature and FVC. The land surface temperature in the regions with a distance of more than 25 km from the coastline decreases obviously with increasing FVC in summer. However, the correlation between the land surface temperature and FVC showed a slight change in the winter period. (3) UHI ratio index decreases along with the area of high FVC (H-FVC) area. The influence of ocean climate on seasons is different, which results in the reduced effect of the H-FVC area and differences in the UHI ratio index. (4) The distance from the coastline should be considered as an important factor in the forestry development planning of the coastal cities.
Pollen Development and Viability in Diploid and Doubled Diploid Citrus Species
Authors: Lora Jorge , Garcia-Lor Andres , Aleza Pablo
Published: April 2022

Seedlessness is one of the most important agronomic traits in mandarins on the fresh fruit market. Creation of triploid plants is an important breeding strategy for development of new commercial varieties of seedless citrus. To this end, one strategy is to perform sexual hybridizations, with tetraploid genotypes as male parents. However, while seed development has been widely studied in citrus, knowledge of key steps such as microsporogenesis and microgametogenesis, is scarce, especially in polyploids. Therefore, we performed a study on the effect of ploidy level on pollen development by including diploid and tetraploid (double diploid) genotypes with different degrees of pollen performance. A comprehensive study on the pollen ontogeny of diploid and doubled diploid “Sanguinelli” blood orange and “Clemenules” clementine was performed, with focus on pollen grain germination in vitro and in planta, morphology of mature pollen grains by scanning electron microscopy (SEM), cytochemical characterization of carbohydrates by periodic acid–Shiff staining, and specific cell wall components revealed by immunolocalization. During microsporogenesis, the main difference between diploid and doubled diploid genotypes was cell area, which was larger in doubled diploid genotypes. However, after increase in size and vacuolization of microspores, but before mitosis I, doubled diploid “Clemenules” clementine showed drastic differences in shape, cell area, and starch hydrolysis, which resulted in shrinkage of pollen grains. The loss of fertility in doubled diploid “Clemenules” clementine is mainly due to lack of carbohydrate accumulation in pollen during microgametogenesis, especially starch content, which led to pollen grain abortion. All these changes make the pollen of this genotype unviable and very difficult to use as a male parent in sexual hybridization with the objective of recovering large progenies of triploid hybrids.
A Phloem-Expressed PECTATE LYASE-LIKE Gene Promotes Cambium and Xylem Development
Authors: Bush Max , Sethi Vishmita , Sablowski Robert
Published: April 2022

The plant vasculature plays essential roles in the transport of water and nutrients and is composed of xylem and phloem, both of which originate from undifferentiated cells found in the cambium. Development of the different vascular tissues is coordinated by hormonal and peptide signals and culminates in extensive cell wall modifications. Pectins are key cell wall components that are modified during cell growth and differentiation, and pectin fragments function as signals in defence and cell wall integrity pathways, although their role as developmental signals remains tentative. Here, we show that the pectin lyase-like gene PLL12 is required for growth of the vascular bundles in the Arabidopsis inflorescence stem. Although PLL12 was expressed primarily in the phloem, it also affected cambium and xylem growth. Surprisingly, PLL12 overexpression induced ectopic cambium and xylem differentiation in the inflorescence apex and inhibited development of the leaf vasculature. Our results raise the possibility that a cell wall-derived signal produced by PLL12 in the phloem regulates cambium and xylem development.
Meristem Initiation and de novo Stem Cell Formation
Authors: Nicolas Antoine , Laufs Patrick
Published: April 2022

Plant aerial development relies on meristem activity which ensures main body plant axis development during plant life. While the shoot apical meristem (SAM) formed in the embryo only contributes to the main stem, the branched structure observed in many plants relies on axillary meristems (AMs) formed post-embryonically. These AMs initiate from a few cells of the leaf axil that retain meristematic characteristics, increase in number, and finally organize into a structure similar to the SAM. In this review, we will discuss recent findings on de novo establishment of a stem cell population and its regulatory niche, a key step essential for the indeterminate fate of AMs. We stress that de novo stem cell formation is a progressive process, which starts with a transient regulatory network promoting stem cell formation and that is different from the one acting in functional meristems. This transient stage can be called premeristems and we discuss whether this concept can be extended to the formation of meristems other than AMs.
Impact of Social Support Ecosystem on Academic Performance of Children From Low-Income Families: A Moderated Mediation Model
Authors: Wen Xiaoqiong , Li Zhihua
Published: April 2022

This study conducted a questionnaire survey involving 513 children from low-income families (mean age = 13.25 ± 2.19 years) to explore the relationship between social support and academic performance as well as the mediating role of dispositional optimism and the moderating role of grit. A structural equation model analysis showed that: (1) social support has a significant positive predictive effect on academic performance and (2) dispositional optimism has a significant mediating effect on the relationship between social support and academic performance. Further, a moderated mediation effect test showed that grit moderates (3) the direct social support effect on academic performance as well as (4) the direct and indirect pathways among social support, dispositional optimism, and academic performance. The results indicate that social support is conducive to the development of dispositional optimism in children from low-income families, thereby improving their academic performance. At the same time, grit can enhance the positive impact of optimism on the academic performance of children from low-income families. This study has important theoretical and practical implications for effectively improving the academic performance of children from low-income families.
Repetitive Exposure to Orofacial Somatosensory Inputs in Speech Perceptual Training Modulates Vowel Categorization in Speech Perception
Authors: Ito Takayuki , Ogane Rintaro
Published: April 2022

Orofacial somatosensory inputs may play a role in the link between speech perception and production. Given the fact that speech motor learning, which involves paired auditory and somatosensory inputs, results in changes to speech perceptual representations, somatosensory inputs may also be involved in learning or adaptive processes of speech perception. Here we show that repetitive pairing of somatosensory inputs and sounds, such as occurs during speech production and motor learning, can also induce a change of speech perception. We examined whether the category boundary between /ε/ and /a/ was changed as a result of perceptual training with orofacial somatosensory inputs. The experiment consisted of three phases: Baseline, Training, and Aftereffect. In all phases, a vowel identification test was used to identify the perceptual boundary between /ε/ and /a/. In the Baseline and the Aftereffect phase, an adaptive method based on the maximum-likelihood procedure was applied to detect the category boundary using a small number of trials. In the Training phase, we used the method of constant stimuli in order to expose participants to stimulus variants which covered the range between /ε/ and /a/ evenly. In this phase, to mimic the sensory input that accompanies speech production and learning in an experimental group, somatosensory stimulation was applied in the upward direction when the stimulus sound was presented. A control group (CTL) followed the same training procedure in the absence of somatosensory stimulation. When we compared category boundaries prior to and following paired auditory-somatosensory training, the boundary for participants in the experimental group reliably changed in the direction of /ε/, indicating that the participants perceived /a/ more than /ε/ as a consequence of training. In contrast, the CTL did not show any change. Although a limited number of participants were tested, the perceptual shift was reduced and almost eliminated 1 week later. Our data suggest that repetitive exposure of somatosensory inputs in a task that simulates the sensory pairing which occurs during speech production, changes perceptual system and supports the idea that somatosensory inputs play a role in speech perceptual adaptation, probably contributing to the formation of sound representations for speech perception.
Improving the Behavioral Intention of Continuous Online Learning Among Learners in Higher Education During COVID-19
Authors: Xu Wei , Shen Zhi-Yi , Lin Shi-Jia , Chen Jia-Chen
Published: April 2022

The COVID-19 pandemic caused colleges and universities to rely heavily on online learning to continue knowledge dissemination to learners. This study used the second-generation model of unified theory of acceptance and use of technology (UTAUT2) to comprehensively analyze the mediating effects of self-efficacy, which affects learners’ effective use of online tools for learning, and capability of metacognition and self-regulation, which can independently adjust learning progress into the UTAUT2 model, on the learner’s willingness to continue online learning [i.e., their behavioral intention (BI)] by constructing a UTAUT2-based e-learning model. This study administered questionnaires to undergraduates in universities in East China to collect data. The effects of performance expectancy, effort expectancy (EE), social influence (SI), and facilitating conditions (FCs), hedonic motivation (HM), price value (PV), and habits on BI (directly or through mediators) were analyzed through data analysis and structural equation modeling, and the UTAUT2-based e-learning model was accordingly modified. The results indicated that the self-efficacy enhanced the effects of EE, SI, FCs, HM, and PV on learners’ BI; that metacognition and self-regulation (MS) capabilities enhanced the effects of EE on learners’ BI; and that habits had a direct and strong effect on BI. This study also provided some suggestions to enhance higher education learners’ willingness to continue online learning, such as improving social recognition and support, careful design of teaching content, easy-to-use technology, financial support. These results and suggestions may guide colleges and universities in conducting, continuing, or enhancing online education, particularly as the pandemic continues.
Leashes, Litterboxes, and Lifelines: Exploring Volunteer-Based Pet Care Assistance Programs for Older Adults
Authors: McLennan Kate , Rock Melanie J. , Mattos Emma , Toohey Ann M.
Published: April 2022

At the convergence of population aging and pet-ownership, community stakeholders are well-positioned to support older adults’ relationships with companion animals through age-related transitions in health and living arrangements. In this study’s setting, a volunteer-based pet care assistance program launched in 2017 to provide practical assistance with pet care for socially disadvantaged, community-dwelling older adults. This case study explored the impacts and feasibility of this and similar programs via (i) an Internet-based environmental scan to compare similar programs and (ii) qualitative interviews with a sampling of diverse community stakeholders (n= 9). A small number of comparable international programs (n= 16) were found. Among these, programs were delivered using a range of funding models; fewer than half involved collaborations across human social services and animal welfare sectors; and none addressed all dimensions of support offered by our local program. Analysis of qualitative interviews highlighted five major themes confirming the value of the volunteer-based approach and the importance of cross-sectoral collaborations in addressing older adults’ under-recognized pet care-related needs. Taken together, the findings confirmed the effectiveness of our local program model. Collaborative, cross-sectoral programs that target both human and companion animal well-being hold promise to reduce barriers to pet ownership that many disadvantaged older adults face. This unique approach leverages the health-promoting potential of human-animal relationships in ways that enhance quality of life for individuals, animal welfare, and age-friendliness of communities.
Implementation Facilitators and Challenges of a Place-Based Intervention to Reduce Health Disparities in Harlem Through Community Activation and Mobilization
Authors: VanDevanter Nancy , Zhong Lynna , Dannefer Rachel , Manyindo Noel , Walker Sterling , Otero Victor , Smith Kimberly , Keita Rose , Thorpe Lorna , Drackett Elizabeth , Seidl Lois , Brown-Dudley La'Shawn , Earle Katherine , Islam Nadia
Published: April 2022

Background

To address significant health inequities experienced by residents of public housing in East and Central Harlem compared to other New Yorkers, NYC Department of Health and Mental Health (DOHMH) collaborated with community and academic organizations and the New York City Housing Authority to develop a place-based initiative to address chronic diseases in five housing developments, including a community activation and mobilization component led by community health organizers (CHOs).

Purpose

Guided by the Consolidated Framework for Implementation Research (CFIR), we evaluated the initial implementation of the community activation and mobilization component to systematically investigate factors that could influence the successful implementation of the intervention.

Methods

Nineteen in-depth qualitative interviews were conducted with a purposive sample of CHOs, community members and leaders, collaborating agencies and DOHMH staff. Interviews were transcribed verbatim, and themes and codes were developed to identify theoretically important concepts of the CFIR and emergent analytic patterns.

Results

Findings identified important facilitators to implementation: positive community perception of the program, CHO engagement and responsiveness to community needs, CHO norms and values and adaptability of DOHMH and CHOs to community needs. Challenges included the instability of the program in the first year, limited ability to address housing related issues, concerns about long term funding, competing community priorities, low expectations by the community for the program, time and labor intensity to build trust within the community, and the dual roles of CHOs as community advocates and DOHMH employees.

Conclusions

Findings will guide future community activation and mobilization activities. The study demonstrates the value of integrating implementation science and health equity frameworks.
Spatio-Temporal Distribution and Demographic Characteristics of Congenital Heart Defects in Guangdong, China, 2016–2020
Authors: Miao Huazhang , Zeng Qinghui , Shi Zengping , Xia Yi , Shi Lushaobo , Chen Dongxue , Guo Pi , Zhu Yingxian , Wang Dong
Published: April 2022

Background

Congenital heart defects are the most common type of birth defects and bring a heavy disease burden in China. Examining the temporal and spatial trends of congenital heart defects epidemics can give some elementary knowledge for succeeding studies.

Objective

To characterize the spatial-temporal patterns of the prevalence of congenital heart defects based on a substantial cohort of the perinatal fetus in south China in 2016–2020.

Methods

This study was a retrospective population-based cohort study conducted in Guangdong, China from 2016 to 2020. Pregnant women and their infants received birth defect surveillance during pregnancy and seven days after delivery in more than 1,900 midwifery hospitals in 21 cities. Perinatal infants with congenital heart defects were identified and enrolled. The prevalence of congenital heart defects was calculated according to cities, years, urban and rural areas, regions of Guangdong, categories of maternal age at delivery, seasons of delivery, and infant's gender.

Results

A total of 8,653,206 perinatal infants and 53,912 total congenital heart defects were monitored in Guangdong, including 46,716 (86.65%) without other defects and 7,736 (13.35%) with other defects. The average prevalence of total congenital heart defects was 62.30/10,000 (95% CI, 61.78/10,000–62.83/10,000), congenital heart defects without other defects was 53.36/10,000 (95% CI, 52.88/10,000–53.85/10,000), and congenital heart defects with other defects was 8.94/10,000 (95%CI, 8.74/10,000–9.14/10,000). From 2016 to 2020, the prevalence of total congenital heart defects was 54.92/10,000, 54.23/10,000, 63.79/10,000, 73.11/10,000, 68.20/10,000, respectively. We observed geographical variations within the prevalence of congenital heart defects. The prevalence of congenital heart defects was much higher in the Pearl River Delta region than in the non-Pearl River Delta region, as well as higher in urban areas than in rural areas.

Conclusion

The findings of this study are helpful to the understanding of the etiology and epidemiology characteristics of congenital heart defects in south China. Our data likely reflect a better estimate of the spatiotemporal trends in congenital heart defects prevalence than reported previously.
Can Neuropeptide S Be an Indicator for Assessing Anxiety in Psychiatric Disorders?
Authors: Markiewicz-Gospodarek Agnieszka , Kuszta Piotr , Baj Jacek , Dobrowolska Beata , Markiewicz Renata
Published: April 2022

Neuropeptide S (NPS) is a neuropeptide primarily produced within three brainstem regions including locus coeruleus, trigeminal nerve nucleus, and lateral parabrachial nucleus. NPS is involved in the central regulation of stress, fear, and cognitive integration. NPS is a mediator of behavior, seeking food, and the proliferation of new adipocytes in the setting of obesity. So far, current research of NPS is only limited to animal models; data regarding its functions in humans is still scarce. Animal studies showed that anxiety and appetite might be suppressed by the action of NPS. The discovery of this neuromodulator peptide is effective considering its strong anxiolytic action, which has the potential to be an interesting therapeutic option in treating neuropsychiatric disorders. In this article, we aimed to analyze the pharmaceutical properties of NPS as well as its influence on several neurophysiological aspects—modulation of behavior, association with obesity, as well as its potential application in rehabilitation and treatment of psychiatric disorders.
Technological Innovation and Agrifood Systems Resilience: The Potential and Perils of Three Different Strategies
Authors: Charatsari Chrysanthi , Lioutas Evagelos D. , De Rosa Marcello , Vecchio Yari
Published: April 2022

The interest in technological innovation has burgeoned in recent years. Theory and research support the vital role of innovative technologies in enhancing agrifood systems resilience. In this theoretical contribution, focusing on different sets of technologies, we present three technological innovation strategies, discuss their potential for strengthening resilience, and expose some open issues that need to be addressed. Responsible technological innovation arose as a response to the growing concerns about the possible unintended impacts of mega-technological trends, like digital farming tools or nanotechnologies, on agrifood systems. Although responsibly innovating is far from easy, and despite the gaps between theoretical ideals and innovation praxis, responsible technological innovation is a promising development since it can prevent counterintuitive effects of technologies on resilience. On the other hand, poly-innovation emerged as a social practice in which internetworking technologies facilitate—and create bundles with—organizational, social, and business innovation. In that strategy, technology represents a mediator of resilience-enhancing social behavior. However, by promoting the uberization of agrifood systems, poly-innovation is associated with various uncertainties. Finally, micro-innovation refers to the incremental adaptations of existing technologies or the development of new ones through bricolage and tinkering. The commercialization of such innovations through open design can increase the resilience of small-scale farming, especially in low-income countries. Nonetheless, the lack of financial resources, technical assistance, and institutional support hamper the full exploitation of micro-innovation.
Certification of Natural Wine: Policy Controversies and Future Prospects
Authors: Alonso González Pablo , Parga Dans Eva , Fuentes Fernández Rosana
Published: April 2022

Natural wine is made from grapes produced under organic or biodynamic management without using additives in the cellar. Natural wine represents a movement of winegrowers that see agriculture as an ethical act against wine industrialization and as a way to make food systems more sustainable. The movement has gained fast-growing global fame and connects rural producers with urban consumers. The recent French recognition of a natural wine certification has spurred discontent among other European countries and wine business associations. A debate about the policy implications of natural wine certification is necessary in order to shed light on the matter. This article calls for transparency in the labeling of wine ingredients, in line with recent consumer demands, which would make the creation of new certifications redundant.
The Evaluation of the Diagnostic Value of a PCR Assay When Compared to a Serologic Micro-Agglutination Test for Canine Leptospirosis
Authors: Martin Elizabeth A. , Heseltine Johanna C. , Creevy Kate E.
Published: April 2022

Objective

To evaluate PCR assay sensitivity and specificity compared to that of microscopic agglutination test (MAT) for diagnosis of canine leptospirosis. Electronic records search was performed to identify dogs with results for both PCR and MAT testing for leptospirosis.

Methods

All dogs were clinically ill. Diagnosis of leptospirosis was defined as an unvaccinated dog with a positive MAT titer of ≥1:800 or a vaccinated dog or dog with an unknown vaccination status with a positive MAT titer of ≥1:1,600. Diagnosis of leptospirosis was excluded based on MAT titer <1:800 on both the initial and convalescent samples or an initial MAT titer <1:800 and an alternative definitive diagnosis.

Results

Forty-nine samples (urine, n = 39; blood, n = 10) were evaluated. Leptospirosis was diagnosed in 17 dogs and excluded in 26 dogs. Urine PCR assay demonstrated sensitivity of 69.2%, specificity of 100%, positive predictive value of 100%, and negative predictive value of 86.6%. Blood PCR assay demonstrated sensitivity of 25%, specificity of 100%, positive predictive value of 100%, and negative predictive value of 25%. Overall PCR sensitivity was 52.4%, specificity was 100%, positive predictive value was 100%, and negative predictive value was 73.7%.

Conclusions

PCR assay performed on urine or blood has high specificity and positive predictive value when compared to MAT for diagnosis of clinical canine leptospirosis. Sensitivity and negative predictive value are moderate to low, so PCR testing should be performed in conjunction with paired MAT testing for canine leptospirosis. Prior antibiotic therapy does not preclude the use of the PCR test.
Proposed Canadian Consensus Guidelines on Osteoarthritis Treatment Based on OA-COAST Stages 1–4
Authors: Mosley Conny , Edwards Tara , Romano Laura , Truchetti Geoffrey , Dunbar Laurie , Schiller Teresa , Gibson Tom , Bruce Charles , Troncy Eric
Published: April 2022

The Canadian consensus guidelines on OA treatment were created from a diverse group of experts, with a strong clinical and/or academic background in treating OA in dogs. The document is a summary of the treatment recommendations made by the group, with treatments being divided into either a core or secondary recommendation. Each treatment or modality is then summarized in the context of available research based support and clinical experience, as the treatment of OA continues to be a multimodal and commonly a multidisciplinary as well as individualized approach. The guidelines aim to help clinicians by providing clear and clinically relevant information about treatment options based on COAST defined OA stages 1–4.