bluuebunny/crossref_metadata_2025 huggingface dataset:
Mapeamento dos fragmentos florestais da Guarnição da Aeronáutica de Pirassununga (Estado de São Paulo, Brasil)
Authors: Fernandes Emmanuélly Maria de Souza , Sebastiani Renata , Sais Adriana Cavalieri
Published: September 2022

A vegetação remanescente do Estado de São Paulo é fragmentada e tem sofrido com o processo acelerado de devastação, assim a manutenção de fragmentos florestais é essencial para conservação da biodiversidade. Por essa razão, o objetivo desse trabalho foi compreender a dinâmica da vegetação, por meio do mapeamento e análise dos fragmentos florestais que compõem a Guarnição da Aeronáutica de Pirassununga (SP, Brasil), localizada às margens do Rio Mogi Guaçu. Os fragmentos florestais presentes na Guarnição são essenciais para conservação da biodiversidade, uma vez que constituem ecótonos com distintas formações vegetais. A classificação e obtenção da métrica do componente arbóreo deu-se por visitas a campo e pela utilização do software livre QGIS 2.18, através do qual também foi possível realizar o exame das imagens do satélite Landsat 8, e o cálculo dos índices de vegetação por diferença normalizada (NDVI) e de forma (SI). A partir desse estudo identificou-se aproximadamente 2000 hectares de vegetação nativa, considerando formações como Floresta Estacional Semidecidual (FES) associada à Floresta Ciliar, Cerrado e Cerradão, e áreas de transição, além dos 252 hectares de várzea e 356 hectares de silvicultura. Dos municípios pertencentes à Unidade de Gerenciamento de Recursos Hídricos da Bacia Hidrográfica do Rio Mogi Guaçu, Pirassununga detém fragmentos de FES e Cerradão, com área superior a 200 hectares, sendo este ainda não contabilizado na literatura. E pela utilização do NDVI pode-se constatar que no verão a vegetação apresentou-se mais densa e que no inverno há a redução desses valores devido à diminuição da biomassa foliar.
Dietary and oral hygiene patterns as risk factors for oral health in a Brazilian city without water fluoridation system
Authors: Moura Helene Soares , Leite Dayane Franco Barros Mangueira , Alves Danielle da Nóbrega , Freitas Claudia Helena Soares de Morais , Costa Maria José de Carvalho , Sampaio Fábio Correia
Published: September 2022

The objective was to investigate dietary and oral hygiene patterns as risk factors for dental caries, fluorosis and tooth erosion in a subsample of individuals of a population-based study in the city of João Pessoa (without water fluoridation program), Brazil. In this cross-sectional study data were collected through clinical examinations and questionnaires addressing dietary and oral hygiene habits. The clinical examination was performed by two calibrated examiners (Kappa > 0.80). The sample (n = 244) was distributed among four age groups: G1 (0-13 years), G2 (14-30 years), G3 (31-54 years) and G4 (≥ 55 years). The prevalence of dental caries was high (87.4%) whereas fluorosis and erosion was low (4.1% and 6.1%, respectively). The mean DMFT index was 1.49 ± 2.19 in G1, 8.19 ± 6.10 in G2, 18.19 ± 7.00 in G3 and 25.81 ± 6.80 in G4. Significant associations were found between the DMFT index and both diet (p = 0.05) and income (p = 0.022), suggesting an associated effect of these variables. Oral hygiene was not related to dental caries experience.  Cariogenic diet was related to the high DMFT index in all age groups supporting that dietary pattern was a stronger risk factor for caries experience in contrast to oral hygiene habits that no association was found.
Sensoriamento Remoto aplicado à análise do fenômeno de Ilhas de Calor na Cidade de Juazeiro do Norte - CE
Authors: Freitas Cley Anderson Silva de , Moreira Luis Clenio Jario , Calou Vinícius Bitencourt Campos , Figueiredo José Vidal de , Sousa Raissa Rodrigues de , Sampaio Gabriel Guerreiro Chaves , Saraiva Kleiton Rocha , Carvalho Clayton Moura de , Silva Manoel Rodrigues da
Published: September 2022

O município de Juazeiro do Norte, localizado na região do Cariri cearense, é uma cidade que se expandiu consideravelmente nas últimas décadas. O processo de urbanização acelerado, sem um planejamento ambiental, tem proporcionado aumento da temperatura do ar e da superfície, afetando diretamente o conforto térmico da população local. Deste modo, o estudo da espacialização e comportamento sazonal desse fenômeno é fundamental para tomadas de decisões nas políticas públicas da cidade. O objetivo deste trabalho, portanto, avaliar o efeito da dinâmica de uso do solo na temperatura superficial da cidade de Juazeiro do Norte visando identificar ilhas de calor e frescor em uma série temporal (2005 a 2020) de imagens da plataforma Landsat.. Para obtenção da série histórica da temperatura de superfície e NDVI foram utilizadas imagens do Landsat 5, sensor TM nas bandas 3, 4 e 6 e Landsat 8, sensor OLI, nas bandas 4, 5 e 10 adquiridas da United States Geological Survey (USGS). Como resultados, foram identificadas ilhas de calor que envolvem os bairros intensamente edificados e com classes de solos expostos, enquanto as ilhas de frescor compõem os corpos hídricos e a vegetação que os margeiam. A arborização da cidade com plantas perenifólias pode favorecer a redução da temperatura local principalmente nos meses de estiagem.
A bibliometric analysis of the relationship between Digital Twins and Health Management: based on the Web of Science (WoS) platform
Authors: Ribeiro Anderson de Oliveira , Sabbadini Francisco Santos , Costa Kelly Alonso , Cruz Bruna Sacramento de Souza
Published: September 2022

Amidst the development of Industry 4.0, the appropriation of digital tools applied to production and manufacturing of activities represents a challenge for managers in other areas. Digital Twin (DT) technology is based on the integration of different "traditional" tools, such as simulation modeling and sensors, and aims to increase the performance of any process that can be represented virtually. With the increase in population, the demand for more efficient and universal Health Management (HM) has become a challenge of the 21st century. This study aims to analyze the relationship between the field of knowledge DT and HM and their interactions. A bibliometric review was performed using the Web of Science database through the Bibliometrix package and the VOSviewer application to evaluate studies, applications and identify research clusters and future trends. Our study indicates that the applications of DT in HM are focused on the diagnosis and monitoring of chronic diseases and that, so far, there is not a critical mass of knowledge that consolidates a general theory of application of DT and HM. This study identifies a relational hotspot between the integration of a DT in the optimization of resource management and patient care.
A eficácia terapêutica dos probióticos na acne: revisão integrativa
Authors: Vasconcellos Isabela Ribeiro Nascimento de , Cipolli Gabriela Cabett , Rossi Raul Cartagena
Published: September 2022

Os probióticos são microrganismos vivos que afetam beneficamente o hospedeiro quando administrados em quantidades adequadas, além de terem um excelente perfil de segurança. Por esse motivo, os probióticos têm sido usados como imunomoduladores em doenças inflamatórias da pele, como a acne inflamatória. Este estudo teve como objetivo avaliar a ação terapêutica do probiótico na acne. Realizou-se uma revisão integrativa da literatura. Dados quantitativos e qualitativos realizados em meio de cultura e em humanos. As buscas eletrônicas foram realizadas no PubMed/MEDLINE e BIREME, considerando os estudos publicados sem limite de data. A busca ocorreu em Outubro de 2020, utilizando uma combinação dos seguintes termos e correlatos: “probiotic” e “acne”. Foram incluídos dez estudos, sendo quatro estudos in vivo e seis in vitro. Os estudos utilizaram vários probióticos conhecidos na literatura para suplementação, obtendo resultado positivo. Entretanto, ainda são escassos os estudos que utilizam o probiótico como um tratamento para acne como coadjuvante com antibiótico ou sozinho.
Panorama do ensino em cuidados críticos na graduação em Enfermagem: uma revisão integrativa
Authors: Menezes João Daniel de Souza , Pereira Amanda Alves , Guarnieri Giovanna Marques , Chiminez Hericlys Matheus , Rodrigues Ana Maria da Silveira , Rodrigues Cléa Dometilde Soares
Published: September 2022

O profissional enfermeiro, responsável por executar os cuidados de enfermagem em ambientes no qual é ofertado assistência em saúde, com leis e regulamentos próprios. A enfermagem possui três categorias, sendo o enfermeiro, profissional com curso de educação superior, assim, ensino em faculdades de enfermagem deve compreender desde o ensino de conteúdos base até cuidados críticos em enfermagem. Para tornar o ensino assertivo o emprego de tecnologias se faz necessário, como uso de recursos controlados de simulação, o que proporciona ao aluno vivência prática sem danos ao paciente e com menor índice de lacunas no ensino. Metodologia: trata-se de um estudo de revisão integrativa da literatura, com uso de critérios pré definidos para escolha e seleção dos artigos. Resultados: após o uso dos critérios pré estabelecidos, encontrou-se um total de 23 artigos. Assim, após a leitura foi optado por subdivisões para melhor discussão em relação à temática do ensino em cuidados críticos, dessa forma a discussão foi contextualizada em subtemas sendo: Relação do pensamento crítico e tomada de decisão durante a graduação; Uso de ambiente virtual para ensino; Uso de ambiente controlado presencial no ensino crítico. Conclusão: o ensino de habilidade críticas na atualidade se torna facilitado por recursos tecnológicos, os quais permitem ao graduando desenvolver habilidades e competências técnicas, emocionais e aprimorar o relacionamento interprofissional, com objetivo de reduzir danos e experiências negativas ao aluno e também ao cliente final dessa formação, o paciente.
Análise epidemiológica dos casos de violência sexual no Estado do Maranhão entre 2015 a 2020
Authors: Costa Lucas Manoel Oliveira , Sousa Jéssica Lima , Chaves Verônica Priscila Macedo , Lima Maria do Amparo Paranã , Souza Emerson Silva , Oliveira Aline Custódia Ayres de , Santos Daniele Anchieta dos , Santos Lisiane Pires Martins dos , Souza Marcus Vinicius de Carvalho , Rosal Veronésia Maria de Sena
Published: September 2022

Objetivo: analisar os casos notificados de violência sexual ocorridos no Estado do Maranhão dentro de um recorte temporal. Metodologia: trata-se de um estudo epidemiológico do tipo transversal, descritivo, retrospectivo, de abordagem mista, urdido pelos achados dispostos no DataSUS, via Tabnet, registrados por meio do Sistema de Informação de Agravos de Notificação (SINAN) referente ao Estado do Maranhão, nos anos de 2015 a 2020. Utilizou-se dos seguintes variáveis para reger o estudo: faixa etária, sexo (binário), raça e grau de parentesco ou proximidade e anos de notificação. Resultados: houve um registro de 3142 casos, sendo as principais vítimas deste agravo mulheres 93,22%. Além disso, crianças de 10 a 14 anos (36,38%) foram destaque entre os achados, como também casos ocorridos entre a raça parda (72,66%), ano de notificação foi 2020 com 24,44% e e a prevalência de 633 casos cujo o violentador era desconhecido da vítima. Discussão: esta violência é, historicamente, um prevalente agravante no estado de saúde pública, salientando-se o Estado do Maranhão, sendo fomentada pela inegável cultura do estupro e impactando diretamente na saúde física, sexual, psicológica, patrimonial e econômica das vítimas. Conclusão: dada à relevância desta temática, constatou-se no presente estudo a prevalência de casos no público feminino (dentro dos aspectos binários), como pôde-se compreender a perpetuação de um ciclo negativo e vicioso historicamente conhecido. Ademais, houve uma crescente dentre os casos notificados, sinalizando os impactos da utilização dos sistemas de notificação.
Perfil antropométrico de pacientes com síndrome lipodistrófica associada ao HIV em um hospital Amazônico
Authors: Lima Débora Suellen de Oliveira Guimarães , Lima João Gabriel Barbosa , Rosário Diana Domingas Silva do , Lima Ellaine Valéria Araújo da Silva , Aguiar Arthur Ribeiro , Costa Lívia Bessa dos Santos , Braga Ana Carolina da Cruz , Libonati Rosana Maria Feio
Published: September 2022

A síndrome lipodistrófica no HIV é caracterizada por alterações na distribuição da gordura corporal e alterações metabólicas caracterizadas pelo aumento dos lipídios séricos; resistência periférica à insulina. Os questionários aplicados foram: um questionário para caracterização de adultos com HIV / AIDS. A maioria dos entrevistados era do sexo masculino (66%), com idade entre 29 e 73 anos, quanto ao grau de instrução 44% possuíam o ensino médio completo. Eles eram predominantemente de baixo nível socioeconômico. Quanto ao estado civil, apenas 14% eram casados. Quanto as variáveis antropométricas, a média do peso masculino (66,81 kg) foi maior que o do peso feminino (63,3 kg) e quanto a altura apenas 0,01m de diferença de altura entre os dois grupos, sendo os homens de maior estatura que as mulheres. A média do IMC teve uma pequena diferença entre os dois grupos, sendo assim, apresentando magreza apenas 2 pacientes; eutrofia tivemos 22 pacientes; pré obesidade tivemos 19 pacientes; obesidade tivemos 7 pacientes. A pesquisa traz implicações para a prática profissional, com a percepção da importância de observar, aprimorar e intervir para que as demandas dos usuários dos serviços sejam atendidas, visando direcionar ações que visem a sua resolução e fortalecimento do vínculo do paciente com a saúde.
Perfil dos casos notificados de intoxicação exógena em um município cearense no período de 2017 a 2021
Authors: Diógenes Isla Vieira , Evangelista Brenda Pinheiro , Freitas Taynara Silva de , Silva Ivanise Freitas da , Freitas Kerma Márcia de , Duarte Rafael Bezerra
Published: September 2022

Objetivou-se descrever e analisar o perfil epidemiológico dos casos de notificação compulsória de intoxicação exógena no município de Jaguaribe Ceará no período de 2017 a 2021. Trata-se de um estudo transversal, descritivo e de cunho quantitativo, realizado através do levantamento de dados secundários dos casos de intoxicação exógena notificados no Sistema de Informação de Agravos de Notificação (SINAN) no período de 2017 a 2021 no município de Jaguaribe, Ceará. A coleta de dados foi realizada no período de julho de 2022, através do acesso ao site do Portal do Departamento de Informática do Sistema Único de Saúde (DATASUS). Os dados foram organizados e tabulados com o auxílio do software Microsoft Excel (2019), e posteriormente, foram analisados por meio de estatística descritiva e discutidos frente à literatura. A partir da coleta e análise dos dados foi possível identificar 215 casos de notificações de intoxicação exógena no município em estudo. Destes, predominaram notificações relativas a indivíduos do sexo feminino (60,5%), de raça parda (95%), na faixa-etária entre 20 e 39 anos (44.65%), com a escolaridade ignorada/branca (61,7%), apresentando como principal agente tóxico os medicamentos (72,7%), circunstância predominante a tentativa de suicídio (44%), exposição do tipo aguda-única (58,6%) e evolução com cura sem sequela (93,4%). Portanto, verifica-se que a descrição do perfil epidemiológico de um agravo é de suma importância para subsidiar as ações de prevenção, promoção e recuperação da saúde das populações, bem como a formulação de políticas públicas de saúde objetivando diminuir a ocorrência de casos de intoxicação exógena.
Horizontes atuais da ultrassonografia na investigação de nódulos e câncer de tireoide
Authors: Mendes Lucas Ferrari da Silva , Araújo Neto André Joaquim de , Bezerra Camila de Sá , Freitas Neto Aluízio Pereira de , Moreira Jefferson Segundo Dantas , Piauilino Bárbara Cândida Nogueira , Oliveira Isadora Rênia Lucena , Sousa Lourivan Leal de , Nunes Teresa Cristina Reinaldo , Lima Carlos Daniel de Sousa , Brito Silana Rosa Soares , Leal Anne Kaline Marques Portela , Araújo Maria Eduarda da Silva Oliveira , Ribeiro Gabriel de Vasconcelos Pessoa
Published: September 2022

Introdução: Nódulos tireoidianos são lesões que podem ou não estar vinculados a doenças, de curso benigno ou maligno. A sua detecção tem sido ampliada pelos novos métodos de imagem desenvolvidos. O ultrassom é o primeiro instrumento complementar de avaliação por ser um método não invasivo e que possui grau de sensibilidade satisfatório na maioria dos casos. Permite a caracterização de aspectos preditores de malignidade, contribuindo para a correta investigação diagnóstica Objetivo: elencar as inovações e a aplicabilidade do diagnóstico diferencial ultrassonográfico dos nódulos tireoidianos conforme descrição na bibliografia. Metodologia: Foram selecionados 10 artigos a partir da Biblioteca Virtual de Saúde, publicados entre 2017 e 2022, para compor esta revisão integrativa de literatura. Resultados e Discussão: Embora a grande maioria dos nódulos tireoidianos seja de origem benigna, o achado é motivo de alarde para pacientes, afinal a possibilidade de uma neoplasia é facilmente aventada. A punção aspirativa por agulha fina é o passo subsequente na averiguação dos nódulos tireoidianos e permite a coleta de material para exame citopatológico e reconhecimento do tipo celular que compõe o nódulo. A ultrassonografia multimodal pode representar uma possibilidade no estudo de nódulos em que se recaiam ambiguidades em modos isolados. Conclusão: a ultrassonografia é consagrada como artifício indispensável na pesquisa de nódulos tireoidianos. Sua utilização é capaz fornecer atributos objetivos na necessidade de exames invasivos, reduzindo os riscos inerentes de procedimentos desnecessários. Observa-se que a ultrassonografia é capaz de trazer dados que fortuitamente possam identificar lesões em estágios pré-metastáticos, permitindo a análise e intervenção necessárias.
Translation Process of Core Values, Vision, and Mission into The Prescribed Curriculum
Authors:   SMAK HendrikusSurabaya, Indonesia, Tondoprasetyo Antonius Cahyono
Published: September 2022

The case study is intended to describe how Core Values, Vision, and Mission of English Education Department in Widya Mandala Catholic University’s Graduate School (Magister Pendidikan Bahasa Inggris or “MPBI”) are translated into the syllabi (prescribed curriculum) using the Value Sharing Model as the framework and Actor Network Theory (ANT) to explain the process within this framework. The research questions are: first, do the course syllabi reflect the Vision, Mission and Core Values of MPBI? And second, how the vision, mission and core values are translated into the prescribed curriculum (syllabi)? This study was conducted by analyzing the syllabi using document analysis parameters and triangulated by conducting interviews to some lecturers and students in MPBI-19. The results are: first, the syllabi were reflecting the core values, vision and mission statements. Second, there are four ways to deliver values. Third, syllabus should be seen as a part of a curriculum not as a separated unit of course. Fourth, all courses are crystallized in Teaching Practice course (in practical term) and Thesis Writing (in theoretical form). The suggestions were: first, using a generalized format of syllabus to help lecturers state their values explicitly. Second, there are opportunities to research further the same topic in the scope of enacted curriculum. And third, lecturers should maintain their internalization processes.
Student-to-Student Cooperation in Virtual Learning without Breakout Rooms
Authors:   Universitas Pendidikan Ganesha, Singaraja, Bali Indonesia, Santosa Made Hery , Ivone Francisca Maria ,   Universitas Negeri Malang, Malang Indonesia, Jacobs George M ,   James Cook University, Singapore, Flores Jenett C. ,   Senior High School, University of Baguio, Baguio city Philippines
Published: September 2022

Education has increasingly turned to virtual learning in response to greater awareness of the benefits of virtual learning, increased technology to support learning outside of educational institutions, and, sadly, the advent of the COVID-19 pandemic. At the same time, awareness has also increased of the benefits of student-student cooperation, and technology to facilitate that cooperation has multiplied. Unfortunately, not all students have access to that technology. This article shares ideas, both low-tech and high-tech, to enhance the functioning of groups in virtual learning environments.
Michel Serres, Topology and Folded Time in Christopher Nolan's Dunkirk
Authors: Hunt Kevin
Published: September 2022

This article discusses Michel Serres's topological thinking and his approach to space and time from a film studies perspective, specifically looking at connections between Serresian philosophy and the work of Christopher Nolan, using Dunkirk (2017) as an example of folded time. The article provides a selective overview of Serres's topological thinking, which opposes a geometrical approach to space and time, as well as indicating connections between Serresian thought and film studies more broadly. Serres makes frequent use of visual metaphors that rely upon motion, which means discussing Serres's philosophy in relation to film can be a purposeful way to make sense of his topological thinking about spatialized time. Serresian philosophy can bring new ideas to film studies while discussion of Serres's thinking from a film studies perspective can help to visualize some of his philosophical ideas. The article considers similarities with, and distinctions from, Gilles Deleuze's understanding of time in cinema, which is modelled on the work of Henri Bergson. One essential difference is that Serres approaches topological time in relation to space whereas Deleuze only considers topological aspects to time through the virtual qualities of the non-spatialized time-image. Key aims of the article are to contribute to film studies through the application of Serresian thinking in relation to Dunkirk as well as offering a new approach to the recurrent themes of space and time in Nolan's work. The main objective is to present Serresian topological thinking as an alternative approach to space and time in film studies, which includes the capability to potentially complement or enhance ideas developed by Deleuze and Bergson. In keeping with Serresian philosophy this article pursues a new way of thinking about folded time in relation to film, based upon exploring new territory and synthesizing ideas rather than attempting to present a universal argument.
Truth, Beauty and Goodness: Freedom and the Platonic Triad in Eric Rohmer’s Film Theory
Authors: Schelstraete Hanne
Published: September 2022

This article analyses Eric Rohmer’s film theory in the light of the Platonic triad of truth, beauty and goodness, as embodied by the aesthetic philosophy of Kant, Hegel and Schiller. Although his film theory shows affinity with Kant’s ideal of art as a form of natural beauty, I will argue that a broader look at Rohmer’s philosophical foundations is necessary. The point where Rohmer’s film theory deviates from Kant’s triadic philosophy is exactly the point where he approaches the aesthetics of Hegel and Schiller. Turning towards their idealistic philosophy creates a new understanding of Rohmer’s film theory. His ideas on cinema’s ontology and value mirror both Hegel’s emphasis on beauty as the artistic and immediate manifestation of trans-historical truth and Schiller’s belief in beauty as the condition for morality and freedom. Plato’s conceptual and intertwined triad of truth, beauty and goodness lies at the basis of his affinity to 18thcentury aesthetic philosophy. This close affinity raises the question of the relation between film theory and philosophy, and Rohmer’s position in this debate. He enriches his own thoughts with those of philosophers that inspired him, and enriches their legacy with his cinema-related conclusions.
Data Analysis of Russian Disinformation Supply Chains: Finding Propaganda in the U.S. Media Ecosystem in Real Time
Authors: Oates Sarah , Lee Doowan , Knickerbocker David
Published: September 2022

This paper uses artificial intelligence to identify a Russian disinformation narrative and track it to its original sources online. This project, using online content that is collected and categorized by the VAST (Veracity Authentication Systems Technology) OSINT system, identifies and analyzes content associated with Russian propaganda with strategic narrative insights. We use the example of accusations of Nazism in Ukraine, specifically related to the Ukrainian Azov regiment, to demonstrate how different stories within this propaganda narrative appear on far-right U.S. websites. At the same time, our study shows little engagement with these stories in the mainstream U.S. media. This paper demonstrates how to scale human content analysis by using artificial intelligence to analyze how foreign propaganda penetrates the U.S. media ecosystem. Using this technology, we can identify disinformation ‘supply chains’ and hopefully disrupt this supply more effectively than we have in the past.
Development of a highly sensitive Gaussia luciferase immunoprecipitation assay for the detection of antibodies against African swine fever virus
Authors: Ding Jingjing , Yang Jifei , Jiang Daoyuan , Zhou Yanyang , Li Chenxi , Li Yanhua
Published: September 2022

In recent years, African swine fever (ASF) has caused a devastating blow to the swine industry globally. Since no effective vaccine is available, strict biosafety measures and rapid diagnosis are the most effective strategies for ASF control. ASFV p30 is one of the most antigenic viral proteins that have been widely used in the field for serological diagnosis of ASF infection. In this study, we developed a luciferase immunoprecipitation system (LIPS) assay for the detection of ASFV antibodies in pig serum using Gaussia luciferase (GLuc)-tagged p30 as a diagnostic antigen. The optimal GLuc-p30 input of 107 luminance units (LU) and optimal serum dilution factor of 1/100 were set to achieve the highest P/N ratio. Based on 87 ASFV-positive and negative pig sera, the cutoff value of the S/N ratio could be set between 2.298 and 30.59 to achieve 100% sensitivity and 100% specificity. Moreover, the diagnostic sensitivity of this LIPS is comparable to that of a commercial enzyme-linked immunosorbent assay (ELISA) and the specificity of LIPS is even superior to the tested ELISA. In conclusion, we have established a LIPS assay for ASFV antibody detection, which could be a potential method for ASFV diagnosis in laboratories and farms.
Thinking-for-speaking patterns in the L2 classroom: A mindful conceptual engagement approach to teaching motion events
Authors: Aguiló-Mora Francisca , Negueruela-Azarola Eduardo
Published: September 2022

Inspired by sociocultural psychology principles applied to understanding language learning and conceptual development, this participatory action research illustrates a pedagogical intervention to teach motion events as conceptual categories in the Second Language (L2) classroom. The goal is to develop and implement conceptual engagement tasks that promote mindful understanding, application, and internalization of motion events as productive concepts in communicative tasks. This is the basis for a Mindful Conceptual Engagement (MCE) approach to L2 development. We focus on L2 Spanish classroom learners when asking-and-giving-directions. This task is conceptually challenging for First Language (L1) English L2 Spanish learners because it is connected to motion events and how Thinking-for-Speaking (TFS) patterns operate in L2 communication. Twenty-three L2-Spanish students from three different courses participated in the project. A selection of coded data and examples of graphic representations are analyzed. Findings illustrate how to teach motion events conceptually and how MCE promotes conceptual awareness and control of L2 communicative features. We also document the challenges of developing functional conceptual categories of meaning as they only emerge when mindfully applied in conceptual engagement tasks. L2 learners must create their own conceptual representations as learning tools to understand motion events. It is concluded that shifting L1 TFS patterns requires creative and mindful conceptual engagement by L2 learners.
Elucidating the relationship between dyslipidemia and osteoporosis: A multicenter, prospective cohort study protocol
Authors: Wei Xu , Zhang Yili , Sun Chuanrui , Qi Baoyu , Huang Xinyi , Chen Ming , Liu Ning , Sun Kai , Chen Xin , Qin Xiaokuan , Xie Yanming , Zhu Liguo
Published: September 2022

Background

A previous study has shown similar factors in dyslipidemias (DL) and osteoporosis (OP). However, no cohort study has been reported on the association between DL and OP in the postmenopausal population in China. This study aims to provide epidemiological and pathophysiological evidence regarding the association between DL and bone mass and fracture risk.

Methods

This is a multicenter, prospective cohort study that will have approximately 1,100 representative participants enrolled from multiple hospitals or communities in China. They will be divided into two groups according to whether or not they are exposed to dyslipidemia and will be epidemiologically investigated. Each participant will be visited continuously once every year with a minimum follow-up of 3 years to track incidences of OP. Meanwhile, free bone density screening, questionnaires, and blood sample collection will also be completed during this period.

Conclusion

The current study is likely to provide greater insight into the relationship between lipid metabolism and bone metabolism in postmenopausal women. Furthermore, the research result maybe fed into public health strategies with regard to metabolic disease prevention.
Identification of key monocytes/macrophages related gene set of the early-stage abdominal aortic aneurysm by integrated bioinformatics analysis and experimental validation
Authors: Cheng Shuai , Liu Yuanlin , Jing Yuchen , Jiang Bo , Wang Ding , Chu Xiangyu , Jia Longyuan , Xin Shijie
Published: September 2022

Objective

Abdominal aortic aneurysm (AAA) is a lethal peripheral vascular disease. Inflammatory immune cell infiltration is a central part of the pathogenesis of AAA. It’s critical to investigate the molecular mechanisms underlying immune infiltration in early-stage AAA and look for a viable AAA marker.

Methods

In this study, we download several mRNA expression datasets and scRNA-seq datasets of the early-stage AAA models from the NCBI-GEO database. mMCP-counter and CIBERSORT were used to assess immune infiltration in early-stage experimental AAA. The scRNA-seq datasets were then utilized to analyze AAA-related gene modules of monocytes/macrophages infiltrated into the early-stage AAA by Weighted Correlation Network analysis (WGCNA). After that, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) functional enrichment analysis for the module genes was performed by ClusterProfiler. The STRING database was used to create the protein-protein interaction (PPI) network. The Differentially Expressed Genes (DEGs) of the monocytes/macrophages were explored by Limma-Voom and the key gene set were identified. Then We further examined the expression of key genes in the human AAA dataset and built a logistic diagnostic model for distinguishing AAA patients and healthy people. Finally, real-time quantitative polymerase chain reaction (RT-qPCR) and Enzyme Linked Immunosorbent Assay (ELISA) were performed to validate the gene expression and serum protein level between the AAA and healthy donor samples in our cohort.

Results

Monocytes/macrophages were identified as the major immune cells infiltrating the early-stage experimental AAA. After pseudocell construction of monocytes/macrophages from scRNA-seq datasets and WGCNA analysis, four gene modules from two datasets were identified positively related to AAA, mainly enriched in Myeloid Leukocyte Migration, Collagen-Containing Extracellular matrix, and PI3K-Akt signaling pathway by functional enrichment analysis. Thbs1, Clec4e, and Il1b were identified as key genes among the hub genes in the modules, and the high expression of Clec4e, Il1b, and Thbs1 was confirmed in the other datasets. Then, in human AAA transcriptome datasets, the high expression of CLEC4E, IL1B was confirmed and a logistic regression model based on the two gene expressions was built, with an AUC of 0.9 in the train set and 0.79 in the validated set. Additionally, in our cohort, we confirmed the increased serum protein levels of IL-1β and CLEC4E in AAA patients as well as the increased expression of these two genes in AAA aorta samples.

Conclusion

This study identified monocytes/macrophages as the main immune cells infiltrated into the early-stage AAA and constructed a logistic regression model based on monocytes/macrophages related gene set. This study could aid in the early diagnostic of AAA.
Macrophage migration inhibitory factor gene rs755622 G/C polymorphism and coronary artery disease: A meta-analysis of 8,488 participants
Authors: Li Yan-yan , Wang Hui , Zhang Yang-yang
Published: September 2022

Background and aims

Macrophage migration inhibitory factor (MIF) gene rs755622 G/C polymorphism was suggested to be associated with CAD risk. However, due to the different results among the individual studies, no agreement has been reached till now. Therefore, the meta-analysis on the association of MIF gene rs755622 G/C polymorphism with CAD was performed.

Methods and results

The association between them was evaluated by calculating the pooled odds ratios (ORs) and the corresponding 95% confidence intervals (CIs). The random-effects models were used because of the significant heterogeneity among them. In this meta-analysis, 8,488 subjects from 9 studies were included. The MIF gene rs755622 G/C polymorphism was significantly associated with CAD under the allelic (OR: 1.213, 95% CI: 1.039–1.417, P = 0.014), recessive (OR: 1.945, 95% CI: 1.214–3.115, P = 0.006), dominant (OR: 0.781, 95% CI: 0.617–0.989, P = 0.041), homozygous (OR: 2.057, 95% CI: 1.289–3.284, P = 0.003), and additive (OR: 1.327, 95% CI: 1.081–1.630, P = 0.007) genetic models.

Conclusion

MIF gene rs755622 G/C polymorphism was significantly related to CAD, especially in the Chinese population. Persons with the C allele of the MIF gene rs755622 G/C polymorphism might be susceptible to CAD.
The path to a hemocompatible cardiovascular implant: Advances and challenges of current endothelialization strategies
Authors: Exarchos Vasileios , Zacharova Ema , Neuber Sebastian , Giampietro Costanza , Motta Sarah E. , Hinkov Hristian , Emmert Maximilian Y. , Nazari-Shafti Timo Z.
Published: September 2022

Cardiovascular (CV) implants are still associated with thrombogenicity due to insufficient hemocompatibility. Endothelialization of their luminal surface is a promising strategy to increase their hemocompatibility. In this review, we provide a collection of research studies and review articles aiming to summarize the recent efforts on surface modifications of CV implants, including stents, grafts, valves, and ventricular assist devises. We focus in particular on the implementation of micrometer or nanoscale surface modifications, physical characteristics of known biomaterials (such as wetness and stiffness), and surface morphological features (such as gratings, fibers, pores, and pits). We also review how biomechanical signals originating from the endothelial cell for surface interaction can be directed by topography engineering approaches toward the survival of the endothelium and its long-term adaptation. Finally, we summarize the regulatory and economic challenges that may prevent clinical implementation of endothelialized CV implants.
Cellular senescence and abdominal aortic aneurysm: From pathogenesis to therapeutics
Authors: Wang Ding , Hao Xinyu , Jia Longyuan , Jing Yuchen , Jiang Bo , Xin Shijie
Published: September 2022

As China’s population enters the aging stage, the threat of abdominal aortic aneurysm (AAA) mainly in elderly patients is becoming more and more serious. It is of great clinical significance to study the pathogenesis of AAA and explore potential therapeutic targets. The purpose of this paper is to analyze the pathogenesis of AAA from the perspective of cellular senescence: on the basis of clear evidence of cellular senescence in aneurysm wall, we actively elucidate specific molecular and regulatory pathways, and to explore the targeted drugs related to senescence and senescent cells eliminate measures, eventually improve the health of patients with AAA and prolong the life of human beings.
Assessment of the nocturnal boundary layer height based on long-term atmospheric radon measurements
Authors: Kubiak Joanna Aleksandra , Zimnoch Miroslaw
Published: September 2022

The article presents the results of statistical analyzes of meteorological and atmospheric radon concentration measurements carried out in Krakow in the years 2004–2013. Based on the diurnal variability, an estimation of the height of the nocturnal boundary layer was performed and compared with the ERA5 data. The average concentration of radon in the air of that period in Kraków was equal to (5.262 ± 0.017) Bqm−3. The diurnal and seasonal variability of radon concentration was found. The correlation of meteorological parameters calculated based on hourly mean data was analyzed. The correlations obtained were statistically significant, for wind speed (r= -0.38, p<0.001), and air humidity (r=0.33, p<0.001). The possibility of determining of the nocturnal boundary layer height on the basis of radon measurements has been confirmed; however, the calculation model needs to be refined.
Expression of long noncoding RNA Xist is induced by glucocorticoids
Authors: Su Yun , Chen Xing , Zhou Hongyan , Shaw Sean , Chen Jie , Isales Carlos M. , Zhao Jing , Shi Xingming
Published: September 2022

Glucocorticoids (GCs) are potent anti-inflammatory and immunosuppressive agents. However, their clinical usage is limited by severe multisystemic side effects. Glucocorticoid induced osteoporosis results in significant morbidity and mortality but the cellular and molecular mechanisms underlying GC-induced bone loss are not clear. GC use results in decreased osteoblast differentiation with increased marrow adiposity through effects on bone marrow stem cells. GC effects are transduced through its receptor (GR). To identify novel GR regulated genes, we performed RNA sequencing (RNA-Seq) analysis comparing conditional GR knockout mouse made by crossing the floxed GR animal with the Col I promoter-Cre, versus normal floxed GR without Cre, and that testing was specific for Col I promoter active cells, such as bone marrow mesenchymal stem/osteoprogenitor cells (MSCs) and osteoblasts. Results showed 15 upregulated genes (3- to 10-fold) and 70 downregulated genes (-2.7- to -10-fold), with the long noncoding RNA X-inactive specific transcript (Xist) downregulated the most. The differential expression of genes measured by RNA-Seq was validated by qRT-PCR analysis of selected genes and the GC/GR signaling-dependent expression of Xist was further demonstrated by GC (dexamethasone) treatment of GR-deficient MSCs in vitro and by GC injection of C57BL/6 mice (wild-type males and females) in vivo. Our data revealed that the long noncoding RNA Xist is a GR regulated gene and its expression is induced by GC both in vitro and in vivo. To our knowledge, this is the first evidence showing that Xist is transcriptionally regulated by GC/GR signaling.
Secondary hormonal alterations in short-term severe hypothyroidism; in the focus: Apelin and copeptin
Authors: Gergics Marin , Pham-Dobor Gréta , Horváth-Szalai Zoltán , Kőszegi Tamás , Mezősi Emese , Bajnok László
Published: September 2022

Objective

This study aimed to investigate the complex interactions of thyroid hormone, apelin, and copeptin in the fluid–ion homeostasis of patients with severe transitory hypothyroidism.

Methods

In this prospective observational study, 39 patients (ECOG: 0; 11 men, 28 women, mean age: 50.3 ± 14.9 years) were investigated during short-term severe hypothyroidism due to surgical removal of the thyroid gland and after adequate thyroid replacement therapy. In addition to the routinely available lab tests, copeptin and apelin levels were determined using ELISA.

Results

In the hypothyroid state, apelin concentration was lower, while copeptin levels did not differ compared to the euthyroid condition. Apelin showed a positive correlation with copeptin (p = 0.003), sodium (p = 0.002), NT-proBNP (p < 0.001), and fT4 (p < 0.001) and a negative correlation with thyroid-stimulating hormone (TSH) (p < 0.001). In multivariate linear regression models, copeptin and TSH proved to be significant independent predictors of apelin levels, of which TSH had an explanatory power of 48.7%. Aside from apelin, copeptin only correlated with sodium (p = 0.046). Sodium levels were negatively associated with TSH (p = 0.004) and positively with ACTH (p = 0.002) and cortisol (p = 0.047), in addition to copeptin. None of the parameters were independent predictors of serum sodium levels in a multivariate regression model.

Conclusions

In short-term severe hypothyroidism, serum apelin level is markedly decreased, which may predispose susceptible patients to hyponatremia, while the level of copeptin is unchanged. TSH and copeptin are independent predictors of apelin concentration, of which TSH is stronger.
Influence of grooved rib tip structure on tip loss and heat transfer in a gas turbine blade
Authors: Kong Zhiqi , Zhang Tianyi , Du Wei
Published: September 2022

This study focuses on the effects of three groove tip structures (full rib groove tip, partial rib tip on the suction side, and partial rib tip on the pressure side) on tip leakage flow, aerodynamic characteristics of a cascade, and heat transfer in a gas turbine blade. The groove’s width B = 1.6 mm, while the tip clearance is τ = 1.2 mm. Results of the flow parameters, fluid flow, and heat transfer in the recessed channel are discussed. The results show that all ribbed tips obtain more uniform outlet flow angle distribution and higher aerodynamic performance than the plane tips. The total aerodynamic pressure loss of the ribbed tips on the pressure side is the same as that of complete ribbed tips. The evolution mechanisms are different, although both can improve the turbine efficiency. Although the partial rib tip on the pressure side weakens the mixing of the channel vortex and leakage vortex near the trailing edge and has the best control effect on the leakage vortex, the lack of the suction side rib will make it easier for the low-energy fluid to flow into the gap from the front of the suction side, which is not conducive to reducing the leakage flow inside the gap; the full rib tip not only minimizes the tip relative leakage flow and leakage loss but also increases the channel vortex loss. With the complex vortex system in the groove and the rib blocking effect at the leakage outlet, the suction-side rib tip becomes the tip structure with the best leakage flow control effect under the same clearance, but the leakage vortex loss is the highest.
It's time! Ten reasons to start replicating simulation studies
Authors: Lohmann Anna , Astivia Oscar L. O. , Morris Tim P. , Groenwold Rolf H. H.
Published: September 2022

The quantitative analysis of research data is a core element of empirical research. The performance of statistical methods that are used for analyzing empirical data can be evaluated and compared using computer simulations. A single simulation study can influence the analyses of thousands of empirical studies to follow. With great power comes great responsibility. Here, we argue that this responsibility includes replication of simulation studies to ensure a sound foundation for data analytical decisions. Furthermore, being designed, run, and reported by humans, simulation studies face challenges similar to other experimental empirical research and hence should not be exempt from replication attempts. We highlight that the potential replicability of simulation studies is an opportunity quantitative methodology as a field should pay more attention to.
Genome-wide analysis of IQD proteins and ectopic expression of watermelon ClIQD24 in tomato suggests its important role in regulating fruit shape
Authors: Dou Junling , Duan Shixiang , Umer Muhammad Jawad , Xie Kuixi , Wang Yinping , Kang Qishuai , Yang Sen , Yang Luming , Liu Dongming , Liu Lifeng , Zhao Fengli
Published: September 2022

The plant-specific IQ67 domain (IQD) is the largest class of calmodulin targets found in plants, and plays an important role in many biological processes, especially fruit development processes. However, the functional role of IQD proteins in the development of watermelon (Citrullus lanatus) shape remains unknown, as the IQD protein family in watermelon has not been systematically characterized. Herein, we elucidated the gene structures, chromosomal locations, evolutionary divergence, and functions of 35 IQD genes in the watermelon genome. The transcript profiles and quantitative real-time PCR analysis at different stages of fruit development showed that the ClIQD24 gene was highly expressed on 0 days after pollination. Furthermore, we found that the ectopic overexpression of ClIQD24 promoted tomato fruit elongation, thereby revealing the significance of ClIQD24 in the progression of watermelon shape. Our study will serve as a reference for further investigations on the molecular mechanisms underlying watermelon fruit shape formation.
Th17-cells in depression: Implication in multiple sclerosis
Authors: Melnikov Mikhail , Lopatina Anna
Published: September 2022

Depression is one of the most common neuropsychological symptoms of multiple sclerosis. However, in addition to mood disorder, depression can also influence on multiple sclerosis course. The mechanism of this dependence is not fully understood. The recent studies suggest the possible common immune mechanisms in the pathogenesis of depression and multiple sclerosis. In particular, it was shown that along with biogenic amines disturbance, neuroinflammation also play an important role in the pathogenesis of depression. Significant attention is drawn to Th17-cells subsets, which are considered as critical players in the pathogenesis of inflammatory diseases of the central nervous system, including multiple sclerosis. This brief report reviews the literature data on the role of neuroinflammation in the reciprocal influence of multiple sclerosis and depression with focus on Th17-cells, which may underlie pathogenetic mechanisms of both this diseases.