bluuebunny/crossref_metadata_2025 huggingface dataset:
Analisis Studi Kelayakan Usaha Pendirian Ricebowl Ikan Tuna Suwir Mangathat di Malang
Authors: Purnomo Purnomo , Ekawati Yurida , Noya Sunday , Hadi Yuswono , Oktiarso Teguh , Kartika Putrianto Novenda
Published: November 2021

Penelitian  ini  bertujuan  untuk  mengetahui  tingkat  kelayakan  investasi  dalam  pendirian IKM ricebowl ikan tuna yang beralamat di Kelurahan Tegalgondo Kecamatan Karangploso Kabupaten Malang. Penelitian ini dilakukan di industri rumahan bernama IKM Ricebowl Ikan Tuna Suwir, yang merupakan Industri Kecil dan Menengah   pengolah makanan. Terjadinya wabah Covid-19 di tahun 2020 ini mengakibatkan permintaan akan produk IKM menurun karena tempat kuliner yang menjalin kerjasama masih menutup usahanya. Penelitian dilaksanakan pada Bulan Juni - Juli 2020 dengan teknik pengumpulan data berupa observasi, wawancara, dan dokumentasi. Metode yang digunakan yaitu aspek pasar dan pemasaran, aspek teknis dan produksi, aspek organisasi dan manajemen, serta aspek finansial dengan perhitungan kelayakan investasi berupa Payback Period (PP) (Present Value)    usaha  makanan ricebowl akan  menghasilkan  manfaat  bersih sebesar Rp. 276.258.850. dengan umur usaha  4   tahun.  Nilai  IRR (Internal  Rate  of Return) yang diperoleh sebesar 36% > 15, 20% (IRR>DR).   Nilai    ini    menunjukan bahwa tingkat  pengembalian  usaha  IKM ricebowl  dalam kondisi optimal. Analisis pasar dan pemasaran menunjukkan bahwa prospek IKM ricebowl ikan tuna cukup baik, dilihat dari peningkatan jumlah permintaan setiap  tahun.
Gambaran Intensitas Penggunaan Gadget dan Kelainan Refraksi Pada Remaja di tengah Pandemi Covid-19 di JABODETABEK
Authors: Setyawati Ribka Dwi , Haryanti Suci , Sulistyono Benny
Published: November 2021

Intensitas penggunaan gadget semakin meningkat seiring dengan kebutuhan dan animo masyarakat akan informasi yang serba cepat, bukan hanya itu dalam gadget juga banyak aplikasi lain yang mampu memfasilitasi pekerjaan seseorang. Hal ini terjadi tanpa menghiraukan dampak yang ditimbulkan dari sinar biru terhadap kesehatan mata terutama kelainan Refraksi. Melalui progam pengabdian Masyarakat yang dilakukan secara mandiri oleh mahasiswa, dalam pengawasan dosen pembimbing. Pada domisili tempat tinggalnya. Yaitu sekitar JABODETABEK. Penggunaan Gadget pada  303 remaja yang diperiksa oleh mahasiswa melalui program pengabdian masyarakat dengan sesuai domisili mahasiswa yaitu wilayah JABODETABEK. Untuk penggunaan gadget tertinggi pada intensitas 2 jam yaitu 69,64% yaitu penggunaan laptop dan untuk penggunaan smartphone pada intensitas 6 jam yaitu 48,84%. Pada kelainan refraksi yang diderita kebanyak remaja adalah myopia yaitu rabun jauh yaitu sebesar 95,04% , astigmat sebesar 40,26% dan hypermetropia 2,31%. Dengan usia tertinggi yang menderita kelainan refraksi adalah 12 tahun  dan 10 tahun.
Penilaian Risiko Penularan Covid-19 pada Peserta Didik di Kampung Inggris Menggunakan Aplikasi InaRisk
Authors: Tsamroh Dewi Izzatus , Devi Permata Wijaya Nika , Zuhria Sugeha Anisa
Published: November 2021

Covid-19 adalah penyakit yang disebabkan oleh varian virus baru yang kemudian dinamakan SARS-CoV-2. Penyakit ini mewabah dari Wuhan, Hubei, Cina ke seluruh dunia, dan ditetapkan sebagai pandemi oleh WHO, dan di Indonesia sendiri wabah ini ditetapkan sebagai bencana nasional. Pemerintah telah memberlakukan kebijakan untuk menekan penularan covid-19 termasuk penerapan protokol kesehatan yang ketat. Seiring berjalannya waktu, kesadaran masyarakat untuk menerapkan protokol kesehatan semakin berkurang. Tujuan kegiatan pengabdian masyarakat ini adalah untuk mengedukasi masyarakat, khususnya di Kampung Inggris Pare mengenai risiko penularan covid-19, serta penilaian mandiri, dan upaya pencegahan penularan covid-19. Kegiatan ini dilakukan dengan menggunakan metode pendidikan masyarakat. Hasil kegiatan menunjukkan bahwa sebagian besar status risiko penularan covid-19 di Kampung Inggris rendah, yaitu sebesar 56%. Akan tetapi, masih ditemui 40% dengan status risiko sedang, dan 4% dengan status risiko tinggi. Hal ini disebabkan adanya pola perilaku peserta didik di Kampung Inggris yang masih abai dengan protokol kesehatan.
Pendampingan Ekowisata di Desa Kucur Kecamatan Dau Kabupaten Malang
Authors: Stephanus Daniel Sugama , Octavianus Rony Joyo Negoro , Manalu Sahala
Published: November 2021

Desa Kucur mengandalkan lahan pertanian tadah hujan atau lahan yang hanya bisa diolah pada musim penghujan. Sehingga, belum memiliki produk pertanian unggulan.   Produk pertanian Desa Kucur hanyalah produk pertanian palawija seperti jagung, cabe merah, kacang tanah, tebu, cabe kecil dan ubi kayu dengan model pertanian tradisional. Komoditi sayur tidak dapat ditanam di Desa Kucur, khususnya di musim kemarau. Akibat pertanian tadah hujan tersebut banyak petani beralih pekerjaan di luar Desa, beralih ke sektor nonformal sebeagai pekerja bangunan, tukang kayu, dan pekerja serabutan. Metode hidroponik merupakan metode yang memungkinkan petani dapat bercocok tanam sepanjang tahun, karena kebutuhan air lebih sedikit dibandingkan dengan pertanian konvensional. Pendampingan ini mendorong dan mendukung usaha rintisan hidroponik dapat lebih berkembang dan menjadi unggulan desa. Lebih lanjut, usaha rintisan tersebut mampu menginisisasi pembentukan kelompok usaha masyarakat desa dan wisata yang berbasis komunitas. Kondisi ini mendorong Tim Program Pendampingan Desa Mitra (PPDM) Universitas Ma Chung, berpartisipasi mengembangkan kualitas kehidupan masyarakat Desa Kucur, khususnya lewat program Pendampingan Wisata Desa untuk peningkatan ekonomi. Kegiatan PPDM dilakukan dengan metode SLA (Sustainable Livelyhood Approach), PRA (Parsipatory Rutal Appraisal) dan CBT (Community Based Tourism). Capaian tahun ke-1 peningkatan kapasitas Sumber Daya Manusia, Tahun ke-2 Penetrasi Pasar, dan tahun ke-3 penguatan kelembagaan dan keberlanjutan usaha.
Pengembangan Usaha Tusuk Sate Melalui Penerapan Teknologi Tepat Guna
Authors: Nurfarida Iva Nurdiana , Sarwoko Endi
Published: November 2021

Home industry biting bambu merupakan salah satu usaha yang ditekuni masyarakat desa Karanganyar, Kec. Poncokusumo, Kab. Malang, hasil produksinya dijual kepada pengepun untuk dijadikan sebagai bahan baku pembuatan tusuk sate. Proses produksi pembuatan biting bambu dilakukan oleh tenaga manusia secara manual, sehingga kapasitas produksi yang dihasilkan masih rendah, padahal jika ditekuni secara serius bisa meningkatkan pendapatan masyarakat. Metode pelaksanaan kegiatan pengabdian meliputi difusi dan penerapan Ipteks, serta pengembangan kemitraan. Penerapan teknologi tepat guna berupa mesin mesin belah dan serut bambu digunakan sebagai solusi untuk meningkatkan jumlah produksi biting bambu, karena jika menggunakan mesin maka proses produksi bisa dilakukan dengan cepat. Peningkatan produksi biting bambu tersebut selanjutnya didukung dengan adanya kemitraan antara pengrajin dengan pengusaha tusuk sate dalam bentuk kemitraan sub-kontrak, dimana pengrajin akan memproduksi biting bambu untuk memenuhi kebutuhan produksi pengusaha tusuk sate sesuai dengan kualitas yang ditetapkan. Penggunaan teknologi berupa mesin belah dan serut bambu menyebabkan peningkatan kapasitas produksi pengrajin antara 50%-60% per bulan, dampaknya pendapatan para pengrajin juga meningkat. Pola kemitraan yang dibangun memberikan keuntungan bagi pengrajin dimana hasil produksi semuanya akan dibeli oleh perusahan mitra dengan harga yang sudah disepakati, bagi perusahaan tusuk sate sebagai mitra, akan membantu perekonomian masyarakat sekitar. Penerapan teknologi tepat guna dengan kemitraan menjadi salah satu model yang bisa diadopsi oleh pemerintah daerah untuk pengembangan usaha kreatif dalam rangka meningkatkan pendapatan masyarakat.
Pengembangan Kemampuan Enterpreneur Sebagai Upaya Meningkatkan Competitive Advantage UMKM
Authors: Ekasari Ratna , Setiawan Setiawan , Wulandari Anita , Kunsiati Wahyu , Novitasari Mariyana
Published: November 2021

Dalam rangka mengembangkan kemampuan entrepreneur untuk meningkatkan daya saing melalui UMKM, maka Universitas Maarif Hasyim Latif memberikan bekal tambahan bagi pengusaha kecil dan menengah untuk menghadapi persaingan di dunia usaha. Salah satu usaha yang dilakukan adalah melalui penyuluhan cara pembuatan laporan keuangan yang benar. Program ini dilaksanakan melalui  pendampingan tentang pembuatan laporan keuangan (Laporan Laba Rugi, Laporan Perubahan Modal, Neraca, Laporan Arus Kas, dan Catatan atas Laporan Keuangan) dengan tujuan untuk mengembangkan UMKM agar mampu bersaing dalam dunia usaha di pasar nasional. Berdasarkan pengamatan dari 7 UMKM yang ada, hampir 85% belum bisa membuat laporan keuangan. Sedangkan pengelolaan keuangan hanya berdasarkan pemasukan dan pengeluaran tanpa ada pemisahan antara modal usaha dan modal pribadi.
Cycle System of Product Industrial Activities Berbasis Iptek dengan Mengoptimalisasikan Sumber Daya Lokal Potensi Kulit Kopi untuk Desa Sejahtera Kemiri
Authors: Amarawardani Nisfatul , Arsyad Haydar Nur , Izzah Dewi Jalinan , Hidayatullah M. Furqon , Rizkitasari Lindia
Published: November 2021

Desa Kemiri, Kecamatan Jabung Kabupaten Malang merupakan salah satu desa penghasil kopi terbesar di Kabupaten Malang. Pengelolaan kopi memberikan hasil samping berupa kulit kopi (Cascara) yang berpotensi menjadi limbah. Alternatif solusi yang bisa dilakukan yaitu dengan cara mengolah limbah kulit kopi (Cascara) tersebut menjadi produk olahan minuman seduh (teh) yang bisa dimanfaatkan oleh masyarakat sekitar, terutama untuk kelompok PKK Desa Kemiri sebagai Usaha Mikro, Kecil dan Menengah (UMKM). Program Cycle System Of Product Industrial Activities (CSPIA) Berbasis IPTEK ini bertujuan untuk memberdayakan kelompok PKK Desa Kemiri melalui pemanfaatan kulit kopi (Cascara). Metode yang digunakan yaitu metode terencana dengan beberapa tahapan yaitu persiapan, pelaksanaan, evaluasi dan penyusunan laporan. Pada tahap pelaksanaan ini diakukan pelatihan Cycle System Of Product Industrial Activities (CSPIA) berbasis IPTEK, pendampingan kewirausahaan, dan pengenalan UMKM. Hasil dari pelaksanaan program ini adalah meningkatkan pengetahuan dan keterampilan kelompok PKK tentang pengolahan limbah kulit kopi (Cascara) menjadi produk olahan minuman seduh (teh) yang dapat  memberikan nilai ekonomis pada kulit kopi.
Pemberdayaan Masyarakat dalam Penanaman Tanaman Melati (Jasminum Sambac) Sebagai Larvasida Alami untuk Pencegahan Demam Berdarah Dengue (DBD) di Desa Lebakjabung Kec. Jatirejo Kab. Mojokerto
Authors: Charisma Acivrida Mega , Anwari Farida , Farida Elis Anita , Wahyuni Khurin In
Published: November 2021

Demam Berdarah Dengue (DBD) masih merupakan salah satu masalah kesehatan masyarakat yang utama di Indonesia. DBD  disebabkan oleh salah satu virus dengue berbeda dan ditularkan melalui nyamuk terutama Aedes aegypti dan Aedes albopictus yang ditemukan pada daerah tropis dan sub tropis di antaranya kepulauan Indonesia hingga bagian utara Australia (WHO, 2009). Menurut Kementerian Kesehatan Republik Indonesia dalam sebulan pada bulan Januari-Februari 2016 ditemukan sebanyak 8.487 kasus di Indonesia dengan jumlah kematian sebanyak 108 orang. Golongan terbanyak yang mengalami DBD di Indonesia pada usia 5-14 tahun mencapai 43,44% dan usia 15-44 tahun mencapai 33,25%. Kejadian Luar Biasa (KLB) penyakit DBD di Indonesia tersebar pada 11 provinsi.  Di Mojokerto Jawa Timur terdapat peningkatan hampir 2.5 kali lipat dibandingkan dengan periode yang sama tahun 2015. Pada tahun 2016, kasus DBD di Sidoarjo hampir mencapai 1% dari angka kasus nasional sebesar 155.927 (Kompas, 2016). Tingginya angka kasus DBD khususnya di daerah Mojokerto mengindikasikan bahwa pemberantasan DBD masih belum maksimal. Terlebih, metode yang di anggap paling efektif untuk mengendalikan nyamuk vektor demam berdarah adalah dengan cara membunuh jentik- jentiknya. Cara alternatif yang paling aman yaitu dengan cara menggunakan bahan alami yang mudah terurai di alam, termasuk melalui metode penanaman tanaman melati (jasminum sambac) sebagai larvasida alami sehingga tidak mencemari lingkungan serta relatif aman bagi manusia dan ternak peliharaan.
Sayur Berkah di Masa Pandemi
Authors: Chosinawarotin Chosinawarotin , Sudrajat Hendrig
Published: November 2021

Dilaksanakannya kegiatan pengabdian kepada masyarakat berupa pemberian sayur gratis diharapkan dapat terjalin silaturahmi dan persaudaraan antar sesame sehingga terbentuk rasa kekerabatan antara masyarakat dengan civitas akademik UNISBA Blitar. Adapun tujuan dari kegiatan yang dilakukan ini adalah untuk membantu masyarakat yang terkena dampak Covid-19 dan masyarakat lanjut usia dengan harapan dapat meringankan beban kebutuhan masyarakat. Hasil kegiatan pengabdian ini yaitu proses pembagian sayur gratis dilakukan secara langsung dengan cara menempatan sayur gratis di pinggir jalan yang biasanya dilewati oleh warga. Untuk lebih memudahkan dalam pengambilan,anggota pengabdian menempatkan sayur gratis di jalan dekat mushola yang kemudian setelah selesai jamaah Sholat Asyar masyarakat dengan semangat mengambil sayur gratis yang sudah disediakan.
Pembuatan Handsanitizer Secara Ekonomis dan Mandiri sebagai Pencegahan Covid-19 pada Masyarakat Blitar
Authors: Suprayitno Hery , Sunarsasi Sunarsasi , Arinanda Kurnia Denny
Published: November 2021

Hand sanitizer (antiseptik tangan) adalah produk kesehatan yang secara instant dapat mematikan kuman tanpa menggunakan air, dapat digunakan kapan saja dan dimana saja, misalnya setelah memegang uang, sebelum makan, setelah dari toilet dan setelah membuang sampah. Merebaknya COVID-19 tersebut membuat masyarakat melakukan panicbuying terhadap kebutuhan pokok dan alat kesehatan tak terkecuali masker dan hand sanitizer, sehingga menyebabkan ketersediaan barang tersebut menjadi langka dan mahal. Pelaksanaan program pelatihan ini dibagi menjadi beberapa tahapan yang meliputi sosialisasi, pelaksanaan dan evaluasi. Hasil dari kegiatan pengabdian masyarakat ini adalah Pelatihan pembuatan handsanitizer dengan alat manual telah dilakukan, sehingga warga dapat berkarya menggunakan bahan lainnya. Terjadi peningkatan pengetahuan dan keterampilan baik dari tim kelompok maupun warga setempat. Warga mampu membuat handsanitizer berbahan alam yang aman untuk kesehatan.
Gaya Kepemimpinan Direktur Utama, Disiplin Kerja, dan Motivasi Kerja Terhadap Kinerja Karyawan
Authors: Purnamasari Wulan , Syaifudin Moch Dawud , Ali Mukti
Published: November 2021

Didalam penelitian ini terdapat maksud identifikasi pengaruh peran gaya kepemimpinan direktur utama, kinerja karyawan, disiplin kerja dan motivasi kerja pada kinerja karyawan pada PT. X Surabaya. Didalam penelitian ini menggunakan 53 responden karyawan yang memiliki pekerjaan dengan jabatan tetap pada PT. X Surabaya karena menggunakan metode purposive sampling. Pengolahan data pada penelitian ini menggunakan metode statistika Patrial Least Square (PLS) dalam proses pengujian hipotesis dan pengaruh dari variabel – variabel yang ada, seperti gaya kepemimpinan Direktur utama (gaya kepemimpinan partisipatif, gaya kepemimpinan delegatif dan gaya kepemimpinan otoriter), disiplin kerja (hubungan kemanusiaan, teladan kepemimpinan dan sanksi hukum) dan motiovasi kerja (kebutuhan akan keselamatan, keamanan, kebutuhan sosial dan juga kebutuhan fisik) kinerja karyawan (kualitas kerja dan kuantitas kerja)

Hasil dari penelitian didapatkan dari T-Statistic dan P-Value dengan presentase nilai(<0.05) maka dari data tersebut diperoleh variabel gaya kepemimpinan Direktur utama pada motivasi kerja (0,462), variabel gaya kepemimpinan Direktur utama pada kinerja karyawan (0,387), motivasi kerja pada kinerja karyawan (0,388), disiplin kerja pada motivasi kerja (0.525) dan disiplin kerja pada kinerja karyawan (0.034). Dan didapatkan kesimpulan hasil yaitu yang berpengaruh positif signifikan adalah disiplin kerja pada kinerja karyawan pada perolehan presentase di bawah p-value (0,05) yaitu (0,034)
Analisis Peramalan Permintaan Darah di Unit Transfusi Darah Kota Madiun
Authors: Khoiri Halwa Annisa , Muttaqin Aan Zainal , Elyuda Dika Restu
Published: November 2021

Unit Donor Darah (UDD) Kota Madiun melayani transfusi darah sekaligus menyediakan darah dari permintaan yang berasal dari Bank Darah Rumah Sakit (BDRS) maupun langsung dari Rumah Sakit di wilayah Kota Madiun dan sekitarnya. Pada tahun 2020, UDD telah berhasil memenuhi 97% permintaan darah, namun karena penyebaran virus Covid-19 yang semakin meluas maka diperlukan peramalan permintaan darah sampai akhir tahun 2021. Berdasarkan analisis data permintaan pada tahun sebelumnya diperoleh model peramalan terbaik untuk permintaan darah A adalah SARIMA(2,1,0)(0,1,1)12 dengan nilai MAPE 9,35%, model peramalan untuk permintaan darah B adalah SARIMA(1,1,0)(0,1,1)12 dengan MAPE 13,25%, model peramalan untuk permintaan darah AB adalah ARIMA(2,1,1) dengan MAPE 22,46%, dan model peramalan untuk permintaan darah O adalah ARIMA(0,1,1).
Usulan Kinerja Green Logistic dengan Pendekatan Root Cause Analysis guna Meningkatkan Re- Order Point yang Efektif
Authors: Dewi Lolyka , Komari Ana , Lutfianto Saufik
Published: November 2021

Konsep manajemen persediaan akan dipuncakkan untuk mencapai green logistic. Pertumbuhan ekonomi mengakibatkan perusahaan manufaktur harus menerapkan efisiensi yang ramah lingkungan. Efisiensi ramah lingkungan tidak hanya fokus pada lingkungan sekitar perusahaan. Efisiensi ramah lingkungan menggunakan pendekatan Green Logistic demi mencapai kinerja optimal. Green logistic sebagai puncak akhir pencapaian dalam konsep manajemen persediaan. Tujuan penelitian 1) untuk membandingkan nilai pemesanan kembali secara eksisting dengan metode Re-Order Point pada Preform Clear 600 ml, 2) untuk meningkatkan peran Green Logistic pada Preform Clear 600 ml. Metode analisis data diawali dengan mengitung nilai Re – Order Point dibandingkan dengan kondisi pemesanan secara eksisting. Tahap kedua menggunakan metode FMEA untuk mengetahui indikator dengan nilai RPN tertinggi ranking 1, 2 dan 3. Tahap ketiga membuat diagram fishbone dengan melakukan brainstroming untuk mengetahui usulan yang tepat dalam mencapai green logistic. Penelitian ini menghasilkan bahwa Perbandingan nilai pemesanan kembali secara eksisting sebesar 2.000.000 buah preform 600ml sedangkan metode Re-Order Point sebesar 2.284.174 pada Preform Clear 600 ml, sehingga efektifitas laba dengan acuan  Re-Order Point lebih menguntungkan, 2) Peran Green Logistic pada Preform Clear 600 ml dengan usulan pengembangan Monitoring dan pelatihan pekerja di bidang logistik perlu di tingkatkan 1 bulan, evaluasi persediaan lebih baik menerapkan metode analisis Re – Order Point dan meningkatkan jadwal kedatangan preform 600 ml dengan langkah meeting pra- kedatangan preform 600 ml
Nilai Perlambatan Dan Uji Ketegangan Disch Brake Pada Sistem Pengereman (Gokart 7,5 Hp)
Authors: Tripariyanto Afiff Yudha , Dewi Lolyka , Komari Ana
Published: November 2021

Setiap kendaraan baik kendaraan yang kita pakai sehari hari sampai ke kendaraan sport untuk balapan di lintasan akan dilengkapi dengan system Pengereman. Pengereman merupakan salah satu piranti penting dalam sebuah kendaraan bermotor baik Roda dua maupun roda empat dan seterusnya. Secara sederhana Fungsi dari pengereman untuk mengurangi laju kendaraan dari kecepatan tinggi ke rendah dan sampai benar-benar berhenti. Dalam system pengereman dibagai menjadi 2 yaitu Disch brake dan Drum brake yang membedakan keduanya terletak pada cara kerjanya dan jenis komponenaya. Metode yang digunakan dalam penelitian ini adalah menggunakan metode secara langsung yaitu mulai mempersiapkan alat dan bahan serta melakukan pengujian secara langsung yaitu pemasangan pengeraman Disch brake pada Gokart Daiho 7,5 Hp yang peletakanya dilakukan pada poros belakang dengan menghitung centroid/titik tengah sehingga didapatkan balancing yang sesuai.  Dalam penelitian ini akan menganalisa aplikasi nilai perlambatan pengereman Disch brake pada Gokart Daiho 7,5 Hp dengan beberapa variasi kecepatan awal yang berbeda yitu dimulai dari nilai kecepatan terkecil 20,30,40,50,60 dengan nilai perlambatan pengereman adalah 80,75,94,11,125 dan 150m/dtk dengan waktu pengereman 0,25. 0,4. 0,425.0,4.0,4 dan 0,4. Dengan efisiensi pengereman sebesar 13,58% dengan nilai tegangan pengereman sebesar 0,26m². dari hasil nilai tersebut maka bisa ditarik kesimpulan bahwa semakin tinggi nilai kecapatan kendaraan maka juga akan semakin lama waktu yng digunakan untuk memberhrntikan kendaraan tersebut dan jarak pengeremanpun juga akan semakin panjang.
Customer Segmentation berdasarkan Usia, Jumlah Kredit dan Lama Kredit Nasabah di Bank XYZ menggunakan Model K-Means Clustering
Authors: Wijaya Moch Rizky , Wibowo Gigih Satriyo
Published: November 2021

Pelanggan internet banking tumbuh sangat cepat. Segmentasi nasabah dapat diterapkan berdasarkan data internet banking. Clustering adalah teknik data mining tanpa pengawasan yang dapat digunakan untuk segmentasi pelanggan. Penelitian ini membangun model clustering pada data profil nasabah bank berdasarkan data kredit sehinagga didapatkan segemntasi nasabah yang nantinya digunakan sebagai landasan keputusan untuk melakukan startegi pemasaran. Metode clustering menggunakan metode K-Means dengan validasi cluster menggunakan metode Silhouette coefficient. Berdasarkan Silhouette coefficient didapatkan nilai terbaik untuk 3 cluster yaitu cluster 0, 1, dan 2. Hasil cluster dengan k-means terbagi menjadi 3 cluster yaitu cluster 0 – rata-rata jumlah kredit lebih rendah, durasi pendek, dan pelanggan usia tua, cluster 1 – rata-rata jumlah kredit tinggi, durasi panjang, dan pelanggan paruh baya atau usai pertengahan, dan cluster 2 - rata-rata jumlah kredit lebih rendah, durasi pendek, dan pelanggan usia muda. Hasil segementasi tersebut dapat dijadikan acuan untuk melakukan startegi pemasaran kedepan.
Klugee: Web-Based Application for Teaching Mathematics in English to Indonesian Elementary School Students
Authors: Amiroh Bebasari
Published: November 2021

Despite the advancement of digital technology, there is no application specializing in teaching mathematics in English for Indonesian elementary school learners. During the pandemic, teachers need online teaching media to assist them when teaching mathematics.  This article covers the project of teaching media creation for teaching Common Core mathematics to Indonesian elementary school students. The objectives of this project are creating web-based applications focusing to assist teachers to teach Mathematics in English for Indonesian Elementary students; and providing tools for the teachers in terms of material management such as curriculum and exercises; student management; and step-by-step guide during teaching in the classroom setting. Content and Language Integrated Learning (CLIL) is the teaching method supported by the teaching media. The results of the project are two web-based applications. The first application is the Learning Application which contains the mathematics teaching materials and student management. The second application is the Teaching Guide application, which contains the step-by-step-guide for teachers in CLIL classroom setting. The informal trials with 13 elementary students and 4 mathematics teachers as the participants showed positive results.
The Mediation Effect of Overall Brand Equity between Brand Superiority and Media Consumption Behaviours of Sport Team Consumers During COVID-19
Authors: Yağiz Kadir
Published: November 2021

Background. During the COVID-19 pandemic, it is paramount to investigate how to influence professional sport team consumers’ behaviours towards media consumption, which is an indirect consumption of sports products with importance raised by the lockdown. The effect of brand superiority, one of the essential consumer judgments about the brands, on some behavioural intentions of sport team consumers was examined before; however, the role of brand superiority and overall brand equity on media consumption behaviours in pandemic conditions lacked empirical investigation. Therefore, this study aims to examine the relationship between brand superiority, overall brand equity and media consumption behaviours among professional sports teams’ consumers during the COVID-19 pandemic.

Methods. The participants of this quantitative study were 232 volunteer football (soccer) consumers who support a team in the Turkish Super Football League. The data was collected in 3 weeks using the online convenience sampling method. Confirmatory factor analysis (CFA) and structural equation modelling (SEM) were used in data analyses, and direct and indirect paths tested by the bootstrapping method.

Results. The results of the study showed that brand superiority did not have a direct effect on media consumption behaviours of sport team consumers. Besides, overall brand equity had a direct effect on media consumption behaviours and mediated the relationship between brand superiority and media consumption behaviours.

Conclusions. The evidence in this study indicates sport managers that the cognitive judgement of sport consumers, generally related to the more functional aspect of a sports product alone not an efficient way to directly influence sport team consumers toward media consumption behaviours even in COVID-19 pandemic. Brand equity, however, found to be an effective tool to address for directing sport team consumers’ behaviours toward media consumption in the extraordinary circumstance currently exist.

 Keywords: branding, sports industry, spectator sports, professional sports teams, sports consumers.
Muslim Integration and the Hijabi Monologues Ireland
Authors: Townsend Sarah L.
Published: November 2021

In 2012 and 2013, two productions of the Hijabi Monologues, an American theatre project featuring the stories of Muslim women, were staged in Ireland. This essay considers their relationship to state-sponsored and community-led interculturalism during the Celtic Tiger and post-Tiger years. Both productions centred on the act of storytelling and tended to downplay xenophobia, instead enacting the type of feel-good intercultural exchange that has dominated Irish and European integration efforts since the late 1990s. At the same time, the 2013 production, on which the essay focuses, employed coalition-building strategies borrowed from the field of migrant activism, thereby ensuring Muslim involvement throughout the production process. The Hijabi Monologues Ireland furnishes a snapshot of a transitional moment in Irish intercultural programming when the state-funded projects of the Celtic Tiger era were giving way to migrant-led initiatives. By examining the production's artistic process, community participation, and funding streams, the essay assesses its successes and shortcomings in addressing the complex challenges of Muslim integration.
Buenavistita en Maryland. Un enclave étnico en la migración internacional
Authors: Núñez Aguilar Jorge Iván , Salazar Tania Cruz , Cejudo Espinoza Luis
Published: November 2021

This paper aims to show the sociocultural adaptation strategy of a group of mestizo migrants from Chiapas residing in “Buenavistita,” Laurel, Maryland, U.S. The methodology is qualitative, based on interviews and participant observation. The findings allow us to argue that the migratory experience forces them to loosen their identity boundaries inward the ethnic group to reconstitute themselves, survive away, and maintain their Mexican mestizo status. Resulting in the formation of territorial enclaves where they identified against those who label them as illegal, this being the way to defend themselves in a racialized society. This work contributes empirically to migration studies with the case of Chiapanecos mestizos. Although we conclude that the enclave is for them a refuge area, little can be said about other experiences of migrants from Chiapas around ethnicity and the blend of cultures.
Venite A spasso con noi!
Authors: Kappler Daniela
Published: November 2021

I percorsi didattici A spasso con noi rappresentano un’innovazione sul piano nazionale e internazionale per quanto concerne la declinazione concreta e multimodale del metodo Content and Language Integrated Learning (CLIL) a favore della lingua italiana e per allievi di scuola elementare. I materiali costituiscono i frutti di esperienze didattiche plurime con bambini e adulti e di intense collaborazioni intercantonali e sono dedicati a classi di scuola elementare del Canton Uri. Da agosto 2020 un connubio unico, virtuoso e coerente, di materiali cartacei e digitali ricchi di tasks che lasciano molto spazio alla personalizzazione e alla differenziazione è entrato nelle aule. Il fil rouge che dischiude gli orientamenti del nuovo Piano di studio della Svizzera tedesca e del CLIL è, “naturalmente”, il viaggio. Un viaggio alla scoperta del territorio e della lingua della Svizzera italiana. Il presente articolo ripercorre le esperienze didattiche preparate e vissute all’interno del progetto e ne ritrae il valore formativo per le persone e le istituzioni coinvolte.
Speech Perception through Face Masks by Children and Adults
Authors: Schwarz Julia , Li Katrina (Kechun) , Hong Sim Jasper , Zhang Yixin , Buchanan-Worster Elizabeth , Post Brechtje , Gibson Jenny , McDougall Kirsty
Published: November 2021

Face masks can cause speech processing difficulties. However, it is unclear to what extent these difficulties are caused by the visual obstruction of the speaker’s mouth or by changes of the acoustic signal, and whether the effects can be found regardless of semantic context. In the present study, children and adults performed a cued shadowing task online, repeating the last word of English sentences. Target words were embedded in sentence-final position and manipulated visually, acoustically, and by semantic context (cloze probability). First results from 16 children and 16 adults suggest that processing language through face masks leads to slower responses in both groups, but visual, acoustic, and semantic cues all significantly reduce the mask effect. Although children were less proficient in predictive speech processing overall, they were still able to use semantic cues to compensate for face mask effects in a similar fashion to adults.
Effective Genome Editing in Leishmania (Viannia) braziliensis Stably Expressing Cas9 and T7 RNA Polymerase
Authors: Espada Caroline R. , Quilles José Carlos , Albuquerque-Wendt Andreia , Cruz Mario C. , Beneke Tom , Lorenzon Lucas B. , Gluenz Eva , Cruz Angela K. , Uliana Silvia R. B.
Published: November 2021

Until 2015, loss-of-function studies to elucidate protein function in Leishmania relied on gene disruption through homologous recombination. Then, the CRISPR/Cas9 revolution reached these protozoan parasites allowing efficient genome editing with one round of transfection. In addition, the development of LeishGEdit, a PCR-based toolkit for generating knockouts and tagged lines using CRISPR/Cas9, allowed a more straightforward and effective genome editing. In this system, the plasmid pTB007 is delivered to Leishmania for episomal expression or integration in the β-tubulin locus and for the stable expression of T7 RNA polymerase and Cas9. In South America, and especially in Brazil, Leishmania (Viannia) braziliensis is the most frequent etiological agent of tegumentary leishmaniasis. The L. braziliensis β-tubulin locus presents significant sequence divergence in comparison with Leishmania major, which precludes the efficient integration of pTB007 and the stable expression of Cas9. To overcome this limitation, the L. major β-tubulin sequences, present in the pTB007, were replaced by a Leishmania (Viannia) β-tubulin conserved sequence generating the pTB007_Viannia plasmid. This modification allowed the successful integration of the pTB007_Viannia cassette in the L. braziliensis M2903 genome, and in silico predictions suggest that this can also be achieved in other Viannia species. The activity of Cas9 was evaluated by knocking out the flagellar protein PF16, which caused a phenotype of immobility in these transfectants. Endogenous PF16 was also successfully tagged with mNeonGreen, and an in-locus complementation strategy was employed to return a C-terminally tagged copy of the PF16 gene to the original locus, which resulted in the recovery of swimming capacity. The modified plasmid pTB007_Viannia allowed the integration and stable expression of both T7 RNA polymerase and Cas9 in L. braziliensis and provided an important tool for the study of the biology of this parasite.
Causal Association of Coffee Consumption and Total, Knee, Hip and Self-Reported Osteoarthritis: A Mendelian Randomization Study
Authors: Zhang Yangchang , Fan Jun , Chen Li , Xiong Yang , Wu Tingting , Shen Shisi , Wang Xu , Meng Xuchen , Lu Yanjun , Lei Xun
Published: November 2021

Background

The causal association between coffee consumption and the risk of OA is limited. This study was conducted to identify the potential causal effects of coffee consumption on total, knee, hip, and self-reported OA.

Methods

Genome-wide association studies (GWAS) of OA were derived from the UK Biobank, comprising 50,508 participants of European ancestry (10,083 with cases and 40,425 controls), and genetic data for specific diagnosed knee OA (4462 cases and 17,885 controls), hip OA (12,625 cases and 50,898 controls), and self-reported OA (12,658 cases and 50,898 controls). Primary and secondary genetic instruments (11 SNPs and 8 SNPs) were selected as instrumental variants from GWAS among 375,833 and 91,462 participants. Two-sample Mendelian randomization (MR) analyses were performed to test the effects of the selected single nucleotide polymorphisms (SNPs) and the OA risk. The causal effects were primarily estimated using weighted median and inverse-variance weighted method with several sensitivity analyses.

Results

The MR analyses suggested that genetically predicted 1% increase of coffee consumption was associated with an increased risk of overall OA (OR:1.009, 95% CI:1.003-1.016), knee OA (OR:1.023, 95% CI:1.009-1.038), self-reported OA (OR:1.007, 95% CI:1.003-1.011), but not hip OA (OR: 1.012, 95%CI:0.999-1.024) using primary genetic instruments. Similar results were found when using secondary genetic instruments that genetically predicted coffee consumption (cups/day). Additionally, the sensitivity analyses for leave-one-out methods supported a robust association between exposure traits and OA.

Conclusion

Our findings indicate that genetically predicted coffee consumption exerts a causal effect on total, knee, and self-reported OA risk, but not at the hip. Further research is required to unravel the role of coffee consumption in OA prevention.
Predicting Bone Metastasis Using Gene Expression-Based Machine Learning Models
Authors: Albaradei Somayah , Uludag Mahmut , Thafar Maha A. , Gojobori Takashi , Essack Magbubah , Gao Xin
Published: November 2021

Bone is the most common site of distant metastasis from malignant tumors, with the highest prevalence observed in breast and prostate cancers. Such bone metastases (BM) cause many painful skeletal-related events, such as severe bone pain, pathological fractures, spinal cord compression, and hypercalcemia, with adverse effects on life quality. Many bone-targeting agents developed based on the current understanding of BM onset’s molecular mechanisms dull these adverse effects. However, only a few studies investigated potential predictors of high risk for developing BM, despite such knowledge being critical for early interventions to prevent or delay BM. This work proposes a computational network-based pipeline that incorporates a ML/DL component to predict BM development. Based on the proposed pipeline we constructed several machine learning models. The deep neural network (DNN) model exhibited the highest prediction accuracy (AUC of 92.11%) using the top 34 featured genes ranked by betweenness centrality scores. We further used an entirely separate, “external” TCGA dataset to evaluate the robustness of this DNN model and achieved sensitivity of 85%, specificity of 80%, positive predictive value of 78.10%, negative predictive value of 80%, and AUC of 85.78%. The result shows the models’ way of learning allowed it to zoom in on the featured genes that provide the added benefit of the model displaying generic capabilities, that is, to predict BM for samples from different primary sites. Furthermore, existing experimental evidence provides confidence that about 50% of the 34 hub genes have BM-related functionality, which suggests that these common genetic markers provide vital insight about BM drivers. These findings may prompt the transformation of such a method into an artificial intelligence (AI) diagnostic tool and direct us towards mechanisms that underlie metastasis to bone events.
High Frequencies of Functional Virus-Specific CD4+ T Cells in SARS-CoV-2 Subjects With Olfactory and Taste Disorders
Authors: Mele Dalila , Calastri Anna , Maiorano Eugenia , Cerino Antonella , Sachs Michele , Oliviero Barbara , Mantovani Stefania , Baldanti Fausto , Bruno Raffaele , Benazzo Marco , Grifoni Alba , Sette Alessandro , Mondelli Mario U. , Varchetta Stefania
Published: November 2021

Olfactory and taste disorders (OTD) are commonly found as presenting symptoms of SARS-CoV-2 infection in patients with clinically mild COVID-19. Virus-specific T cells are thought to play an important role in the clearance of SARS-CoV-2; therefore the study of T cell specific immune responses in patients with mild symptoms may help to understand their possible role in protection from severe disease. We evaluated SARS-CoV-2-specific T cell responses to four different peptide megapools covering all SARS-CoV-2 proteins during the acute phase of the disease in 33 individuals with mild or no other symptom beside OTD and in 22 age-matched patients with severe infection. A control group of 15 outpatients with OTD and consistently negative nasopharyngeal SARS-CoV-2 RNA swabs and virus-specific IgG serology was included in the study. Increased frequencies of virus-specific CD4+ and CD8+ T cells were found in SARS-CoV-2 positive patients with OTD compared with those with severe COVID-19 and with SARS-CoV-2 negative OTD individuals. Moreover, enhanced CD4+ and CD8+ T-cell activation induced by SARS-CoV-2 peptides was associated with higher interferon (IFN)γ production. Increased frequencies of Spike (S1/S2)-specific CD4+ T cells showing enhanced IFNγ secretion and granzyme B content were associated with serum spike-specific IgG in the OTD group. In conclusion, patients with SARS-CoV-2 induced OTD develop highly functional virus-specific CD4+ and CD8+ T cells during the symptomatic phase of the disease, suggesting that robust and coordinated T-cell responses provide protection against extension of COVID-19 to the lower respiratory tract.
MicroRNA-124 Promotes Singapore Grouper Iridovirus Replication and Negatively Regulates Innate Immune Response
Authors: Li Pin-Hong , Wang Li-Qun , He Jia-Yang , Zhu Xiang-Long , Huang Wei , Wang Shao-Wen , Qin Qi-Wei , Sun Hong-Yan
Published: November 2021

Viral infections seriously affect the health of organisms including humans. Now, more and more researchers believe that microRNAs (miRNAs), one of the members of the non-coding RNA family, play significant roles in cell biological function, disease occurrence, and immunotherapy. However, the roles of miRNAs in virus infection (entry and replication) and cellular immune response remain poorly understood, especially in low vertebrate fish. In this study, based on the established virus-cell infection model, Singapore grouper iridovirus (SGIV)-infected cells were used to explore the roles of miR-124 of Epinephelus coioides, an economically mariculture fish in southern China and Southeast Asia, in viral infection and host immune responses. The expression level of E. coioides miR-124 was significantly upregulated after SGIV infection; miR-124 cannot significantly affect the entry of SGIV, but the upregulated miR-124 could significantly promote the SGIV-induced cytopathic effects (CPEs), the viral titer, and the expressions of viral genes. The target genes of miR-124 were JNK3/p38α mitogen-activated protein kinase (MAPK). Overexpression of miR-124 could dramatically inhibit the activation of NF-κB/activating protein-1 (AP-1), the transcription of proinflammatory factors, caspase-9/3, and the cell apoptosis. And opposite results happen when the expression of miR-124 was inhibited. The results suggest that E. coioides miR-124 could promote viral replication and negatively regulate host immune response by targeting JNK3/p38α MAPK, which furthers our understanding of virus and host immune interactions.
Environmental Filtering Influences Functional Community Assembly of Epibenthic Communities
Authors: Sutton Lauren , Mueter Franz J. , Bluhm Bodil A. , Iken Katrin
Published: November 2021

Community assembly theory states that species assemble non-randomly as a result of dispersal limitation, biotic interactions, and environmental filtering. Strong environmental filtering likely leads to local assemblages that are similar in their functional trait composition (high trait convergence) while functional trait composition will be less similar (high trait divergence) under weaker environmental filters. We used two Arctic shelves as case studies to examine the relationship between functional community assembly and environmental filtering using the geographically close but functionally and environmentally dissimilar epibenthic communities on the Chukchi and Beaufort Sea shelves. Environmental drivers were compared to functional trait composition and to trait convergence within each shelf. Functional composition in the Chukchi Sea was more strongly correlated with environmental gradients compared to the Beaufort Sea, as shown by a combination of RLQ and fourth corner analyses and community-weighted mean redundancy analyses. In the Chukchi Sea, epibenthic functional composition, particularly body size, reproductive strategy, and several behavioral traits (i.e., feeding habit, living habit, movement), was most strongly related to gradients in percent mud and temperature while body size and larval development were most strongly related to a depth gradient in the Beaufort Sea. The stronger environmental filter in the Chukchi Sea also supported the hypothesized relationship with higher trait convergence, although this relationship was only evident at one end of the observed environmental gradient. Strong environmental filtering generally provides a challenge for biota and can be a barrier for invading species, a growing concern for the Chukchi Sea shelf communities under warming conditions. Weaker environmental filtering, such as on the Beaufort Sea shelf, generally leads to communities that are more structured by biotic interactions, and possibly representing partitioning of resources among species from intermediate disturbance levels. We provide evidence that environmental filtering can structure functional community composition, providing a baseline of how community function could be affected by stressors such as changes in environmental conditions or increased anthropogenic disturbance.
E2 Site Mutations in S Protein Strongly Affect Hepatitis B Surface Antigen Detection in the Occult Hepatitis B Virus
Authors: Wang Hao , Liao Fenfang , Xie Junmo , Gao Wenbo , Wang Min , Huang Jieting , Xu Ru , Liao Qiao , Shan Zhengang , Zheng Yourong , Rong Xia , Li Chengyao , Fu Yongshui
Published: November 2021

The mechanism of occult hepatitis B infection (OBI) has not yet been fully clarified. Our previous research found that novel OBI-related mutation within S protein, E2G, could cause the hepatitis B surface antigen (HBsAg) secretion impairment, which resulted in intracellular accumulation in OBI of genotype B. Here, to further explore the role of E2 site mutations in the occurrence of OBI, we analyzed these site mutations among 119 OBI strains identified from blood donors. Meanwhile, 109 wild-type HBV strains (HBsAg positive/HBV DNA positive) were used as control group. Furthermore, to verify the E2 site mutations, two conservative 1.3-fold full-gene expression vectors of HBV genotype B and C (pHBV1.3B and pHBV1.3C) were constructed. Then, the E2 mutant plasmids on the basis of pHBV1.3B or pHBV1.3C were constructed and transfected into HepG2 cells, respectively. The extracellular and intracellular HBsAg were analyzed by electrochemical luminescence and cellular immunohistochemistry. The structural characteristics of S proteins with or without E2 mutations were analyzed using relevant bioinformatics software. E2 mutations (E2G/A/V/D) existed in 21.8% (26/119) of OBIs, while no E2 mutations were found in the control group. E2G/A/V/D mutations could strongly affect extracellular and intracellular level of HBsAg (p < 0.05). Notably, unlike E2G in genotype B that could cause HBsAg intracellular accumulation and secretion decrease (p < 0.05), E2G in genotype C could lead to a very significant HBsAg decrease both extracellularly (0.46% vs. pHBV1.3C) and intracellularly (11.2% vs. pHBV1.3C) (p < 0.05). Meanwhile, for E2G/A mutations, the relative intracellular HBsAg (110.7–338.3% vs. extracellular) and its fluorescence intensity (1.5–2.4-fold vs. with genotype-matched pHBV1.3B/C) were significantly higher (p < 0.05). Furthermore, N-terminal signal peptides, with a typical cleavage site for peptidase at positions 27 and 28, were exclusively detected in S proteins with secretion-defective mutants (E2G/A). Our findings suggest that: (1) E2G/A/V/D mutations were confirmed to significantly influence the detection of HBsAg, (2) the underlying mechanism of OBI caused by E2G mutation is quite different between genotype B and genotype C, and (3) E2G/A could produce a N-terminal truncated S protein, which might attribute to the HBsAg secretion impairment in the OBIs.
A Novel Strategy Conjugating PD-L1 Polypeptide With Doxorubicin Alleviates Chemotherapeutic Resistance and Enhances Immune Response in Colon Cancer
Authors: Wang Maolin , Shu Xing-sheng , Li Meiqi , Zhang Yilin , Yao Youli , Huang Xiaoyan , Li Jianna , Wei Pengfei , He Zhendan , Lu Jun , Ying Ying
Published: November 2021

Background

Modifying the structure of anti-tumor chemotherapy drug is of significance to enhance the specificity and efficacy of drug-delivery. A novel proteolysis resistant PD-L1-targeted peptide (PPA1) has been reported to bind to PD-L1 and disrupt the PD-1/PD-L1 interaction, thus appearing as an outstanding tumor-targeting modification of synergistic drug conjugate for effective anti-tumor treatment. However, the combination regimen of coupling PD-L1 polypeptide with chemotherapeutic drug in tumoricidal treatment has not been reported thus far.

Methods

We developed a novel synergistic strategy by conjugating PPA1 to doxorubicin (DOX) with a pH sensitive linker that can trigger the release of DOX near acidic tumor tissues. The binding affinity of PPA1-DOX with PD-L1 and the acid-sensitive cleavage of PPA1-DOX were investigated. A mouse xenograft model of colon cancer was used to evaluate the biodistribution, cytotoxicity and anti-tumor activity of PPA1-DOX.

Results

PPA1-DOX construct showed high binding affinity with PD-L1 in vitro and specifically enriched within tumor when administered in vivo. PPA1-DOX exhibited a significantly lower toxicity and a remarkably higher antitumor activity in vivo, as compared with free PPA1, random polypeptide-DOX conjugate, DOX, or 5-FU, respectively. Moreover, increased infiltration of both CD4+ and CD8+ T cells was found in tumors from PPA1-DOX treated mice.

Conclusions

We describe here for the first time that the dual-functional conjugate PPA1-DOX, which consist of the PD-L1-targeted polypeptide that renders both the tumor-specific drug delivery and inhibitory PD-1/PD-L1 immune checkpoint inhibition, and a cytotoxic agent that is released and kills tumor cells once reaching tumor tissues, thus representing a promising therapeutic option for colon cancer with improved efficacy and reduced toxicity.