bluuebunny/crossref_metadata_2025 huggingface dataset:
A CW-CNN regression model-based real-time system for virtual hand control
Authors: Qin Zixuan , He Zixun , Li Yuanhao , Saetia Supat , Koike Yasuharu
Published: December 2022

For upper limb amputees, wearing a myoelectric prosthetic hand is the only way for them to continue normal life. Even until now, the proposal of a high-precision and natural performance real-time control system based on surface electromyography (sEMG) signals is still challenging. Researchers have proposed many strategies for motion classification or regression prediction tasks based on sEMG signals. However, most of them have been limited to offline analysis only. There are even few papers on real-time control based on deep learning models, almost all of which are about motion classification. Rare studies tried to use deep learning-based regression models in real-time control systems for multi-joint angle estimation via sEMG signals. This paper proposed a CW-CNN regression model-based real-time control system for virtual hand control. We designed an Adaptive Kalman Filter to smooth the joint angles output before sending them as control commands to control a virtual hand. Eight healthy participants were invited, and three sessions experiments were conducted on two different days for all of them. During the real-time experiment, we analyzed the joint angles estimation accuracy and computational latency. Moreover, target achievement control (TAC) test was applied to emphasize motion regression in real-time. The experimental results show that the proposed control system has high precision for 3-DOFs motion regression in simultaneously, and the system remains stable and low computational latency. In the future, the proposed real-time control system can be applied to actual prosthetic hand.
Cerebellum as a kernel machine: A novel perspective on expansion recoding in granule cell layer
Authors: Bae Hyojin , Park Sa-Yoon , Kim Sang Jeong , Kim Chang-Eop
Published: December 2022

Sensorimotor information provided by mossy fibers (MF) is mapped to high-dimensional space by a huge number of granule cells (GrC) in the cerebellar cortex’s input layer. Significant studies have demonstrated the computational advantages and primary contributor of this expansion recoding. Here, we propose a novel perspective on the expansion recoding where each GrC serve as a kernel basis function, thereby the cerebellum can operate like a kernel machine that implicitly use high dimensional (even infinite) feature spaces. We highlight that the generation of kernel basis function is indeed biologically plausible scenario, considering that the key idea of kernel machine is to memorize important input patterns. We present potential regimes for developing kernels under constrained resources and discuss the advantages and disadvantages of each regime using various simulation settings.
Efficacy of portable sleep monitoring device in diagnosing central sleep apnea in patients with congestive heart failure
Authors: Tung Pi-Hung , Hsieh Meng-Jer , Chuang Li-Pang , Lin Shih-Wei , Hung Kuo-Chun , Lu Cheng-Hui , Lee Wen-Chen , Hu Han-Chung , Wen Ming-Shien , Chen Ning-Hung
Published: December 2022

Introduction

Central sleep apnea (CSA) is a common and serious comorbidity mainly occurring in patients with heart failure (HF), which tends to be underdiagnosed and has not been widely studied. Overnight polysomnography (PSG) is the gold standard for diagnosing CSA; however, the time and expense of the procedure limit its applicability. Portable monitoring (PM) devices are convenient and easy to use; however, they have not been widely studied as to their effectiveness in detecting CSA in patients with HF. In the current study, we examined the diagnostic value of PM as a screening tool to identify instances of CSA among patients with HF.

Methods

A total of 22 patients under stable heart failure conditions with an ejection fraction of <50% were enrolled. All patients underwent PM and overnight PSG within a narrow time frame. The measurements of the apnea–hypopnea index (AHI), hypopnea index (HI), central apnea index (CAI), and obstructive apnea index (OAI) obtained from PSG, automatic scoring, and manual scoring of PM were recorded. The results obtained from PSG and those from PM (automatic and manual scoring) were compared to assess the accuracy of PM.

Results

Among the patients, CSA in 11 patients was found by PSG. The AHI measurements performed using manual scoring of PM showed a significant correlation with those performed using PSG (r = 0.69; P = 0.01). Nonetheless, mean AHI measurements showed statistically significant differences between PSG and automatic scoring of PM (40.0 vs. 23.7 events/hour, respectively; P < 0.001), as well as between automatic and manual scoring of PM (23.7 vs. 29.5 events/hour; P < 0.001). Central sleep apnea was detected by PM; however, the results were easily misread as obstructive apnea, particularly in automatic scoring.

Conclusion

PM devices could be used to identify instances of central sleep apnea among patients with HF. The results from PM were well-correlated with standard PSG results, and manual scoring was preferable to automated scoring.
Naturally cultured high resistant starch rice improved postprandial glucose levels in patients with type 2 diabetes: A randomized, double-blinded, controlled trial
Authors: Tan Ling-li , Duan Wei-qian , Chen Meng-xue , Mei Ying , Qi Xiao-ya , Zhang Yong
Published: December 2022

Objective

To assess the effect of a novel naturally cultured rice with high resistant starch (RS) on postprandial glycemia in patients with type 2 diabetes compared to ordinary rice.

Design

This study is a randomized, double-blinded controlled trial.

Methods

Patients with type 2 diabetes were recruited, and postprandial glucose levels were measured at 5-time points after the ingestion of one of two types of cooked rice in random order. Paired t-tests were used to compare postprandial blood glucose changes and increment areas under the blood glucose curve between high-RS rice and ordinary rice.

Results

The increments of the postprandial blood glucose levels for high-RS rice were significantly lower than that for ordinary rice, i.e., 2.80 ± 1.38 mmol/L vs. 3.04 ± 1.50 mmol/L (P = 0.043) and 3.94 ± 2.25 mmol/L vs. 4.25 ± 2.29 mmol/L (P = 0.036) at 30 min and 60 min, respectively. The incremental areas under the blood glucose curve for high-RS rice were also significantly lower than that for ordinary rice, i.e., 42.04 ± 20.65 [mmol/(L·min)] vs. 45.53 ± 22.45 [mmol/(L·min)] (P = 0.043), 143.54 ±69.63 [mmol/(L·min)] vs. 155.15 ± 73.53 [mmol/(L·min)] (P = 0.026), and 354.61 ± 191.96 [mmol/(L·min)] vs. 379.78 ± 195.30 [mmol/(L·min)] (P = 0.042) at 30, 60, and 120 min, respectively. Repeated-measures ANOVA showed that postprandial glucose levels were not affected by the test order.

Conclusion

The novel high-RS rice as a staple food when substituting for widely consumed ordinary rice may provide potential health benefits by lowering blood glucose in patients with type 2 diabetes.
Dose-response association of leisure time physical activity with mortality in adults with major chronic diseases
Authors: Sun Jiahong , Wu Han , Zhao Min , Magnussen Costan G. , Xi Bo
Published: December 2022

We aimed to evaluate the association between leisure-time physical activity (PA) and mortality risk in adults with major chronic diseases. A total of 170,579 adults with major chronic diseases aged 30–84 years from the U.S. National Health Interview Surveys (1997–2014) with linkage to the National Death Index (NDI) through December 31, 2015 were included in this study. During a median follow-up of 7.25 years, 36,914 adults with chronic diseases died from all causes, 8,767 died from cardiovascular disease (CVD), and 9,090 died from cancer. Compared with participants with no leisure-time PA, those with a low level (10–59 min/week) of total leisure-time PA had a 23% [hazard ratio (HR) 0.77, 95% confidence interval (CI) 0.73–0.82] reduced risk of all-cause mortality. Adults with higher levels of leisure time had more reduced risk of all-cause mortality, as well as CVD-specific and cancer-specific mortality. Adults with leisure-time PA ≥ 1,500 min/week had more reduced risk of CVD-specific mortality (61%) but less reduced risk of cancer-specific mortality (29%) compared with the reduced risk of all-cause mortality (43%). There was an inversely non-linear dose-response relationship between leisure-time PA and all-cause and cause-specific mortality. Reduced risk of all-cause and cancer-specific mortality between leisure-time light-to-moderate PA and vigorous-intensity PA time were largely comparable. Low and high levels of leisure-time PA showed substantial survival benefits compared with no leisure-time PA in adults with major chronic diseases. The light-to-moderate-intensity leisure-time PA is largely comparable with vigorous PA to provide survival benefits for all-cause and cancer-specific mortality.
Acute malnutrition and its contributing factors among children under-five years in rural kebeles of Shashemene Oromia, Ethiopia
Authors: Shifera Nigusie , Endale Aschalew , Debela Degfachew , Yosef Tewodros
Published: December 2022

Introduction

Globally, more than 52 million under-five years old were wasted; One-third of these children live in Africa. Ethiopia is the seventh country among the ten top countries in which acute malnutrition (AM) is concentrated and currently 10% of under-five children are wasted. Even though Ethiopia has implemented a variety of nutritional interventions, acute malnutrition is still prevalent and spreading at an alarming rate. Therefore, this study aimed to assess the prevalence of acute malnutrition and its contributing factors among children under-five years of age.

Materials and methods

A community-based cross-sectional study was conducted from July 1 to 30, 2018 among 12 randomly selected kebeles. The sample sizes were proportionally allocated to the selected kebeles. A total of 457 mothers/caretakers of under-five children were interviewed using pre-tested structured questionnaires and anthropometric measurements of the children were taken using standard procedures. EPI data version 4.2 was used for data entry and Statistical Package for the Social Sciences (SPSS) Version 21 was used for statistical analysis. The World Health Organization (WHO) Anthro software was used to convert nutritional data indices. Binary logistic regression was used to determine the association between dependent and independent variables. The level of significance was declared at a P-value < 0.05.

Results

The prevalence of acute malnutrition is 19.91% (95%CI; 16.24%, 23.57%) among under-five children. Factors contributing to acute malnutrition were mothers with no antenatal care (ANC) visits [adjusted odds ratio (AOR) = 2.26, 95% CI 1.14–4.46], mothers who had no autonomy in decision-making (AOR = 2.42, 95% CI 1.42–4.12), children with diarrheal disease in the last 2 weeks preceding the survey (AOR = 2.07, 95% CI 1.19–3.59), and not feeding colostrum (AOR = 1.99, 95% CI 1.07–3.71).

Conclusion and recommendation

The prevalence of acute malnutrition is high as compared to other findings in Ethiopia. Moreover, decision-making power, not feeding colostrum, no ANC visit, and a child's history of diarrhea were independent determinants of acute malnutrition. Therefore, the local health department and health extension workers should consider imparting health education for women on nutritional counseling and timely treatment for children with diarrhea. Empowering women's decision-making is also a key element in addressing wasting among under-five children.
Novel MRI deformation-heterogeneity radiomic features are associated with molecular subgroups and overall survival in pediatric medulloblastoma: Preliminary findings from a multi-institutional study
Authors: Iyer Sukanya , Ismail Marwa , Tamrazi Benita , Salloum Ralph , de Blank Peter , Margol Ashley , Correa Ramon , Chen Jonathan , Bera Kaustav , Statsevych Volodymyr , Ho Mai-Lan , Vaidya Pranjal , Verma Ruchika , Hawes Debra , Judkins Alexander , Fu Pingfu , Madabhushi Anant , Tiwari Pallavi
Published: December 2022

Introduction

Medulloblastoma (MB) is a malignant, heterogenous brain tumor. Advances in molecular profiling have led to identifying four molecular subgroups of MB (WNT, SHH, Group 3, Group 4), each with distinct clinical behaviors. We hypothesize that (1) aggressive MB tumors, growing heterogeneously, induce pronounced local structural deformations in the surrounding parenchyma, and (b) these local deformations as captured on Gadolinium (Gd)-enhanced-T1w MRI are independently associated with molecular subgroups, as well as overall survival in MB patients.

Methods

In this work, a total of 88 MB studies from 2 institutions were analyzed. Following tumor delineation, Gd-T1w scan for every patient was registered to a normal age-specific T1w-MRI template via deformable registration. Following patient-atlas registration, local structural deformations in the brain parenchyma were obtained for every patient by computing statistics from deformation magnitudes obtained from every 5mm annular region, 0 < d < 60 mm, where d is the distance from the tumor infiltrating edge.

Results

Multi-class comparison via ANOVA yielded significant differences between deformation magnitudes obtained for Group 3, Group 4, and SHH molecular subgroups, observed up to 60-mm outside the tumor edge. Additionally, Kaplan-Meier survival analysis showed that the local deformation statistics, combined with the current clinical risk-stratification approaches (molecular subgroup information and Chang’s classification), could identify significant differences between high-risk and low-risk survival groups, achieving better performance results than using any of these approaches individually.

Discussion

These preliminary findings suggest there exists significant association of our tumor-induced deformation descriptor with overall survival in MB, and that there could be an added value in using the proposed radiomic descriptor along with the current risk classification approaches, towards more reliable risk assessment in pediatric MB.
The role of Phosphodiesterase-1 and its natural product inhibitors in Alzheimer’s disease: A review
Authors: Ahmad Nazir , Lesa Kaisun Nesa , Sudarmanto Ari , Fakhrudin Nanang , Ikawati Zullies
Published: December 2022

Phosphodiesterase-1 (PDE1) is a versatile enzyme that has surprisingly received considerable attention as a possible therapeutic target in Alzheimer’s disease (AD) because it maintains the homeostasis of 3ʹ,5ʹ-cyclic adenosine monophosphate (cAMP) and 3ʹ,5ʹ-cyclic guanosine monophosphate (cGMP) in the brain. 3ʹ,5ʹ-cyclic adenosine monophosphate and 3ʹ,5ʹ-cyclic guanosine monophosphate are the two key second messengers that regulate a broad range of intracellular processes and neurocognitive functions, specifically memory and cognition, associated with Alzheimer’s disease. However, the lack of available selective drugs on the market poses challenges to identifying the beneficial effects of natural products. The present review focuses on Phosphodiesterase-1 and its isoforms, splicing variants, location, distribution, and function; the role of Phosphodiesterase-1 inhibitors in Alzheimer’s disease; and the use of vinpocetine and natural products as specific Phosphodiesterase-1 inhibitors. Moreover, it aims to provide ongoing updates, identify research gaps, and present future perspectives. This review indicates the potential role of Phosphodiesterase-1 inhibitors in the treatment of neurodegenerative disorders, such as Alzheimer’s disease. Certain clinical trials on the alleviation of Alzheimer’s disease in patients are still in progress. Among de novo outcomes, the employment of Phosphodiesterase-1 inhibitors to treat Alzheimer’s disease is an important advancement given the absence of particular therapies in the pipeline for this highly prevalent disease. To sum up, Phosphodiesterase-1 inhibition has been specifically proposed as a critical therapeutic approach for Alzheimer’s disease. This study provides a comprehensive review on the biological and pharmacological aspects of Phosphodiesterase-1, its role on the Alzheimer’s diseases and its significance as Alzheimer’s disease therapeutic target in drug discovery from natural products. This review will help clinical trials and scientific research exploring new entities for the treatment and prevention of Alzheimer’s disease.
Screening of the active Ingredients in Huanglian Jiedu decoction through amide bond-Immobilized magnetic nanoparticle-assisted cell membrane chromatography
Authors: Liao Fengyun , He Dongmei , Vong Chi Teng , Wang Lisheng , Chen Zhangmei , Zhang Tiejun , Luo Hua , Wang Yitao
Published: December 2022

Graphical Abstract
A KCNB1 gain of function variant causes developmental delay and speech apraxia but not seizures
Authors: Veale Emma L. , Golluscio Alessia , Grand Katheryn , Graham John M. , Mathie Alistair
Published: December 2022

Objective: Numerous pathogenic variants in KCNB1, which encodes the voltage-gated potassium channel, KV2.1, are linked to developmental and epileptic encephalopathies and associated with loss-of-function, -regulation, and -expression of the channel. Here we describe a novel de novo variant (P17T) occurring in the KV2.1 channel that is associated with a gain-of-function (GoF), with altered steady-state inactivation and reduced sensitivity to the selective toxin, guanxitoxin-1E and is clinically associated with neurodevelopmental disorders, without seizures.

Methods: The autosomal dominant variant was identified using whole exome sequencing (WES). The functional effects of the KCNB1 variant on the encoded KV2.1 channel were investigated using whole-cell patch-clamp recordings.

Results: We identified a de novo missense variant in the coding region of the KCNB1 gene, c.49C>A which encodes a p.P17T mutation in the N-terminus of the voltage-gated, KV2.1 potassium channel. Electrophysiological studies measuring the impact of the variant on the functional properties of the channel, identified a gain of current, rightward shifts in the steady-state inactivation curve and reduced sensitivity to the blocker, guanxitoxin-1E.

Interpretation: The clinical evaluation of this KCNB1 mutation describes a novel variant that is associated with global developmental delays, mild hypotonia and joint laxity, but without seizures. Most of the phenotypic features described are reported for other variants of the KCNB1 gene. However, the absence of early-onset epileptic disorders is a much less common occurrence. This lack of seizure activity may be because other variants reported have resulted in loss-of-function of the encoded KV2.1 potassium channel, whereas this variant causes a gain-of-function.
Response strategies of fine root morphology of Cupressus funebris to the different soil environment
Authors: Wen Xiaochen , Wang Xiao , Ye Mengting , Liu Hai , He Wenchun , Wang Yu , Li Tianyi , Zhao Kuangji , Hou Guirong , Chen Gang , Li Xianwei , Fan Chuan
Published: December 2022

Understanding fine root morphology is crucial to uncover water and nutrient acquisition and transposition of fine roots. However, there is still a lack of knowledge regarding how the soil environment affects the fine root morphology of various root orders in the stable forest ecosystem. Therefore, this experiment assessed the response strategies of fine root morphology (first- to fifth -order fine roots) in four different soil environments. The results showed that fine root morphology was related to soil environment, and there were significant differences in specific root length (SRL), specific surface area (SRA), diameter (D), and root tissue density (RTD) of first- and second -order fine roots. Soil total nitrogen (TN), alkaline nitrogen (AN) and available phosphorus (AP) were positively correlated with SRL and SRA and negatively correlated with D and RTD. Soil moisture (SW) was positively correlated with the D and RTD of first- and second-order fine roots and negatively correlated with the SRL and SRA. Soil temperature (ST), organic carbon (OC), soil bulk density (SBD) and soil porosity (SP) were not significantly correlated with the D, SRL, SRA, and RTD of the first- and second -order fine roots. AN was positively correlated with SRL and SRA and negatively correlated with both D and RTD in the first- and second -order fine roots, and the correlation coefficient was very significant. Therefore, we finally concluded that soil AN was the most critical factor affecting root D, SRL, SRA and RTD of fine roots, and mainly affected the morphology of first- and second -order fine roots. In conclusion, our research provides support for understanding the relationship between fine root morphology and soil environment, and indicates that soil nutrient gradient forms good root morphology at intraspecific scale.
The impact of moral leadership on physical education teachers’ innovation behavior: The role of identification with leader and psychological safety
Authors: Chen Jineng , Zheng Wei , Jiang Binbin
Published: December 2022

With the growth of people’s health needs and the impact of the COVID-19 pandemic, it is an inevitable trend to promote innovation behaviors of physical education (PE) teachers to innovate traditional physical education and adapt to national needs of sustainable development in the sports industry. Considering that moral leadership can promote innovation behavior of individuals through psychological factors, this study defines the types of innovation behavior, and from the perspective of psychological safety and identifying with leaders, discusses the impact of moral leadership on individuals’ innovation behavior by using hierarchical multivariate regression analysis, which provides inspiration for schools to strengthen the innovation behavior of physical education teachers. In this study, 327 questionnaires were distributed to PE teachers in Chinese provinces and 287 valid questionnaires were collected. The analysis of the collected data was performed with the help of the SPSSAU data analysis platform. The following conclusions were drawn: First, moral leadership has a significant positive impact on the psychological safety and internal and external innovation of physical education teachers. Secondly, moral leadership influences employees’ innovation behavior through psychological safety, and plays a part of intermediary role between moral leadership and internal and external innovation behavior; Third, by comparing the two impact mechanisms of innovation behavior, we found that moral leadership encourages employees to produce more external innovation behavior through psychological safety; Finally, strong leadership identity plays a positive role in regulating the relationship between moral leadership and innovation behavior.
Is psychological science progressing? Explained variance in PsycINFO articles during the period 1956 to 2022
Authors: Smedslund Geir , Arnulf Jan Ketil , Smedslund Jan
Published: December 2022

We aimed to numerically assess the progress of modern psychological science. Average explained variance in 1565 included articles was 42.8 percent, and this was constant during 1956 to 2022. We explored whether this could be explained by a combination of methodological conventions with the semantic properties of the involved variables. Using latent semantic analysis (LSA) on a random sample of 50 studies from the 1,565, we were able to replicate the possible semantic factor structures of 205 constructs reported in the corresponding articles. We argue that the methodological conventions pertaining to factor structures will lock the possible explained variance within mathematical constraints that will make most statistics cluster around 40 percent explained variance. Hypotheses with close to 100 percent semantic truth value will never be part of any assumed empirical study. Nor will hypotheses approaching zero truth value. Hypotheses with around 40 percent truth value will probably be experienced as empirical and plausible and, consequently, as good candidates for psychological research. Therefore, to the extent that the findings were indeed produced by semantic structures, they could have been known without collecting data. Finally, we try to explain why psychology had to abandon an individual, causal method and switch to studying whether associations among variables at the group level differ from chance. Psychological processes take place in indefinitely complex and irreversibly changing contexts. The prevalent research paradigm seems bound to producing theoretical statements that explain each other to around 40%. Any theoretical progress would need to address and transcend this barrier.
A nomological network for misophonia in two German samples using the S-Five model for misophonia
Authors: Remmert Nico , Jebens Antonia , Gruzman Rebecca , Gregory Jane , Vitoratou Silia
Published: December 2022

The Selective Sound Sensitivity Syndrome Scale (S-Five) is a contemporary and multidimensional self-report instrument measuring different aspects of misophonia. The five-factor scale consists of 25 items measuring the severity of the misophonic experience. The items capture misophonia in relation to internalising and externalising appraisals, perceived threat, aggressive behavior (outbursts), and adverse impact on individuals’ lives. It is complemented by a trigger checklist (S-Five-T), measuring the emotional nature and intensity of reactions to sensory triggers. In this work, we administered the S-Five in two German samples with a majority of individuals with significant misophonia. The S-Five and the supplementary S-Five-T were both translated into German using a rigorous translation procedure (i.e., TRAPD) and were separately tested in large German community samples. Psychometric analyses included the evaluation of the factor structure, measurement invariance with respect to age and gender, reliability (internal consistency and stability over time), and an extensive examination of the construct validity in a proposed nomological network. The nomological network we explore in this work consists of several constructs including different misophonic manifestations, anger and aggression, disgust propensity, anxiety sensitivity, depression, obsessive–compulsive traits, and functional impairment in different life domains. Results indicate evidence in line with the nomological network as demonstrated by strong correlations between the S-Five dimensions and convergent measures. All S-Five dimensions strongly correlated with overall misophonic symptoms (r ≥ 0.53). Internalising appraisals were highly associated with insight into excessive or disproportionate reactions to sounds (r ≥ 0.59), externalising appraisals with anger and irritability (r ≥ 0.46), threat with trait anxiety and dysregulation facets (r ≥ 0.62), aggressive behavior (outbursts) with anger and behavioral dysregulation (r ≥ 0.70), and impact with distress and functional impairment (r ≥ 0.64). The results demonstrate that the S-Five has a robust five-factor structure and allows to draw reliable and valid conclusions about misophonic experiences in German samples. The proposed nomological network gives an initial insight into the nature of misophonia and provides a formalized fundament to develop and test further hypotheses about misophonia in a more sophisticated and symptom-oriented way.
Humble leadership and its outcomes: A meta-analysis
Authors: Luo Yifei , Zhang Zeyu , Chen Qishu , Zhang Kairui , Wang Yijiang , Peng Jianfeng
Published: December 2022

The importance of humble leadership has garnered attention from both researchers and practitioners. Unfortunately, despite the accumulation of recent findings on the effects of leader humility, a quantitative review remains scant. In addressing this void, this study is among the first to conduct a meta-analytic review of humble leadership and its outcomes. Eighty-four correlations (N = 16,534) from 53 independent studies are synthesized. The authors found that: (a) humble leadership is positively related to affective commitment (ρ = 0.56), affective trust (ρ = 0.62), creativity (ρ = 0.39), engagement (ρ = 0.40), leader–member exchange (LMX) (ρ = 0.58), job satisfaction (ρ = 0.51), organizational identification (ρ = 0.48), psychological empowerment (ρ = 0.33), self-efficacy (ρ = 0.24), task performance (ρ = 0.33), and voice (ρ = 0.34); and that (b) humble leadership contributes a significant incremental variance beyond transformational, servant, and ethical leadership in several crucial criterion variables, providing solid evidence for the construct's uniqueness. However, humble leadership does not explain incremental variance in some criterion variables, indicating that future studies should control for the influence of some positive leadership (e.g., transformational and servant leadership). Age, gender, study design, country, and year partially moderate the correlations of interest. We discuss our findings with caution and propose future research directions.
Neutropenia after the coadministration of clozapine and nirmatrelvir-ritonavir in a patient with SARS-CoV-2 infection: A case report with a literature review
Authors: Liu Chun-I , Goh Kah Kheng , Chen Chun-Hsin
Published: December 2022

Background

Schizophrenia is considered one of the major risk factors for mortality from SARS-CoV-2 infection. Early antiviral treatment is important to decrease the risk of mortality. Currently, Paxlovid (nirmatrelvir-ritonavir) has been widely used in SARS-CoV-2 patients with risk factors. However, drug–drug interactions with anti-psychotics are prominent and complicated.

Case presentation

We report a clozapine-treated patient with SARS-CoV-2 infection who developed neutropenia after coadministration with Paxlovid. In this case, clozapine was used for over 15 years, without neutropenia development. However, severe neutropenia (absolute neutrophil count = 523/μl) developed 3 days after the coadministration of Paxlovid 2 doses per day, valproic acid 1,000 mg per day and clozapine 100 mg per day. The development of neutropenia may be attributed to the complicated interaction among Paxlovid, SARS-CoV-2 infection, valproic acid, fluvoxamine and clozapine.

Conclusions

Neutropenia is a rare but life-threatening event if a concomitant infection occurs. The risk may increase during SARS-CoV-2 infection and the coadministration of clozapine and Paxlovid. Although the exact causes of neutropenia in this patient are not fully clear, the white blood cell count and absolute neutrophil count should be closely monitored during the administration of Paxlovid in clozapine-treated patients with SARS-CoV-2 infection.
Urban infrastructure development-human security nexus: Flows, spaces, and livelihoods framework for comparative research in Africa's post-colonies
Authors: Mbiba Beacon
Published: December 2022

This desktop study paper suggests a “flows and livelihoods” framework for comparative studies on displaceability in the context of infrastructure and investment/projects in diverse post-colonial settings. It uses the ongoing upgrading of Mbudzi (Goats) interchange, in Harare, to discuss the utility of this framework in addressing diverse sustainability and human security questions irrespective of scale, scope and settings of the project. Thus, the paper contributes to integrated ways of understanding dynamics and sustainability of infrastructure investments. In the process, it also responds to calls on the need for exemplars on how theory can be integrated into planning research. Ultimately, what it offers is a heuristic device for cross-sectional and time-series studies.
The effect of oregano essential oil on the prevention and treatment of Salmonella pullorum and Salmonella gallinarum infections in commercial Yellow-chicken breeders
Authors: Xu Ziheng , Wang Can , Li Changcheng , Wang Min , Chen Wenyan , Zhou Chenyu , Wei Ping
Published: December 2022

In order to prevent pullorum disease and fowl typhoid in breeders, the use of oregano essential oil (OEO) was tested for the prevention and treatment of infections of multidrug-resistant Salmonella pullorum (SP) and Salmonella gallinarum (SG) in commercial Yellow-chicken breeders. In the challenge-protection experiment, commercial Hongguang-Black 1-day-old breeder chicks were randomly divided into four groups, including A (challenged, preventive dose), B (challenged, treatment dose), C (challenged, untreated), and D (unchallenged, untreated). Group A was supplemented with 200 μL/L OEO in the drinking water during the whole trial (1-35 days of age) and group B was supplemented with 400 μL/L OEO during 8–12 days of age, while groups C and D were kept as untreated controls. At 7 days of age, birds of groups A, B, and C were divided into two subgroups with equal number of birds (A1-A2, B1-B2, and C1-C2), and then subgroups A1, B1, and C1 were challenged with SP, while subgroups A2, B2, and C2 were challenged with SG. Clinical symptoms and death were observed and recorded daily. Every week during the experiment, serum antibodies against SP and SG of all the groups were detected by the plate agglutinate test (PAT). At the age of 35 days, all birds were weighed and necropsied, lesions were recorded and the challenging pathogens were isolated. The results showed that the positive rates of SP and SG isolation in groups A1, A2 and B1, B2 were significantly lower (P < 0.05) than those of groups C1 and C2, respectively, while groups A1 and A2 were slightly lower (P > 0.05) than those of groups B1 and B2. The average body weight (BW) of groups A1 and A2 were significantly higher (P < 0.05) than those of groups B1, B2 and C1, C2, respectively, but there was no significant difference (P > 0.05) with that of group D. The r-value between PAT positive and the recovery rates of Salmonella was 0.99, which means they are highly positively correlated. The results of this study demonstrated that the prevention dose (200μL/L) and the treatment dose (400 μL/L) of OEO supplemented in the drinking water could all effectively decrease infections of SP and SG and that the effect of the prevention was greater than that of the treatment and finally that the prevention could also significantly reduce the BW decline of birds challenged with SP and SG.
Structural Characteristics of a Shock Train Flow Field in a Variable Cross-Section S-Shaped Isolator
Authors: Yan Yuepeng , Fan Xiaoqiang , Xiong Bing
Published: December 2022

Experiments were conducted in this study to reveal the flow characteristics of a variable cross-section S-shaped isolator, when applying the steady-state back pressure at the isolator outlet. The self-excited oscillation characteristics of the shock train generated under the influence of steady-state back pressure at an incoming flow speed of Mach 2, were also studied. The findings suggest that pressure oscillation within the area affected by the shock train’s flow field was significantly more potent than outside the affected area. Moreover, the forward movement velocity of the shock train in the variable cross-section S-shaped isolator was not uniform. The forward movement speed was slower when encountering sharp turns and faster during gentle turns. In the shock train flow field, high-frequency pressure oscillations, which mainly stemmed from the oscillations of the separated shock legs, propagated more readily within the flow field than low-frequency pressure oscillations. The significant separation of the shock train flow field will switch between the top and bottom walls, and the frequency of pressure oscillation in the large separation region is low. On another note, the closer the distance between two points is in the shock flow field, the stronger the coherence of pressure oscillations will be. In the distance upstream of the shock train flow field, the turbulent boundary layer (TBL) determines pressure oscillations instead of the shock train flow field, so the coherence was very high.
Evaluation of the Parasitism Capacity of a Thelytoky Egg Parasitoid on a Serious Rice Pest, Nilaparvata lugens (Stål)
Authors: Shi Longqing , Liu Dawei , Qiu Liangmiao , Jiang Zhaowei , Zhan Zhixiong
Published: December 2022

Pseudoligosita yasumatsui and Anagrus nilaparvatae are both egg parasitoids of the brown planthopper, Nilaparvata lugens (Stål) (BPH). In this study, we obtained a stable strain of P. yasumatsui reproduced via thelytoky through indoor rearing and screening. We assessed the parasitism capacity of this strain on eggs of N. lugens by comparing the parasitism preference and circadian rhythm of this strain to that of A. nilaparvatae, which is proved as the dominant egg parasitoid species of BPH in rice fields. The findings indicated that both egg parasitoids could parasitize fertilized and unfertilized BPH eggs, however, with a significant preference for fertilized eggs. The daily parasitization volume of P. yasumatsui was slightly higher than that of A. nilaparvatae. Both egg parasitoids preferred parasitizing 1–3-day-old BPH eggs, but the parasitism amount of 5–6-day-old BPH eggs by P. yasumatsui is higher than that by A. nilaparvatae. The parasitism events of both species of egg parasitoid wasps occurred primarily from 7:00–15:00 and the parasitism amount at night accounted for less than 15% of the total amount. The results indicate that this strain of P. yasumatsui reproduced via thelytoky could be valuable for rice planthopper control.
Use of Heating Methods and Xylose to Increase Rumen Undegradable Protein of Alternative Protein Sources: 1) Peanut Meal
Authors: Rigon Fernanda , Pereira David A. B. , Loregian Kalista E. , Magnani Elaine , Marcondes Marcos I. , Branco Renata H. , Benedeti Pedro D. B. , Paula Eduardo M.
Published: December 2022

Peanut meal has an excellent total protein content but also has low rumen undegradable protein (RUP). High-performance ruminants have high RUP requirements. We aimed to evaluate the effects of processing peanut meal with an autoclave and conventional and microwave ovens, with and without using xylose on its ruminal kinetics degradation parameters and intestinal digestibility (ID). In situ studies were conducted to determine dry matter (DM) and crude protein (CP) rumen degradation kinetics. In vitro studies were conducted to evaluate intestinal digestibility (ID). The control treatment had a greater fraction A for DM and CP than peanut meals processed with an autoclave or conventional oven. The control had greater kd for CP compared with the microwave. The addition of xylose decreased fraction A, the degradation rate of fraction B (kd) and RUP, and increased the protein B fraction of autoclaved peanut meal. We observed a decrease in effective degradability (ED) and increased RUP for processed treatments in all experiments compared with the control. Processing methods did not affect the protein ID of autoclaved peanut meal compared to the control. An interaction between xylose and heating time was observed, where increasing heating time linearly reduced the ID of xylose-untreated treatments. Overall, these results suggest that the tested methods effectively increased the RUP content of peanut meal.
Analysis of Extended Spectrum Beta Lactamase (ESBL) Genes of Non-Invasive ESBL Enterobacterales in Southeast Austria in 2017
Authors: Paulitsch-Fuchs Astrid H. , Melchior Nadine , Haitzmann Theresa , Fingerhut Theres , Feierl Gebhard , Baumert Rita , Kittinger Clemens , Zarfel Gernot
Published: December 2022

Extended spectrum beta lactamases producing Enterobacteriaceae are a major player in the antibiotic resistance challenge. In general, the situation regarding antibiotic resistance in Austria is very good compared to many other countries. Perhaps this is why there is a lack of data on the distribution of ESBL genes in the clinical setting. The aim of this study was to collect data on ESBL genes from a larger sample of human non-invasive clinical isolates from one region in Austria. In total, 468 isolates from different sample materials isolated at the Medical University of Graz from 2017 were examined. The most frequent organisms were Escherichia coli and Klebsiella pneumoniae. Among the enzymes produced, CTX-M-15 was clearly dominant, exotic ESBLs were only represented by three Proteus mirabilis isolates harboring genes for VEB-6 and one P. mirabilis for CTX-M-2, respectively. Compared to other countries, the results are in line with the expectations. The data help to better classify the many studies from the non-clinical field in Austria and to shift the focus slightly away from the exotic results and sample sites.
Personalized Approach in Eradication of Helicobacter pylori Infection
Authors: Mestrovic Antonio , Perkovic Nikola , Tonkic Ante , Sundov Zeljko , Kumric Marko , Bozic Josko
Published: December 2022

The increase in antibiotic resistance to Helicobacter pylori (H. pylori) is associated with a decrease in the effectiveness of eradication therapy. Although some success has been achieved by adjusting therapeutic regimens according to local data on resistance to certain antibiotics, a new approach is needed to ensure a better therapeutic response. Tailored therapy, based on sensitivity tests to antibiotics, is increasingly proving to be a superior therapeutic option, even as a first-line therapy. Moreover, the recently published Maastricht VI guidelines emphasize utilizing a susceptibility-guided strategy in respect to antibiotic stewardship as the first choice for eradication therapy. In addition, polymerase chain reaction (PCR) technology is becoming a standard tool in the diagnosis of H. pylori infections through non-invasive testing, which further optimizes the eradication process. We provide a review regarding the current position of the individualized approach in eradication therapy and its future prospects. Based on novel understandings, the personalized approach is an effective strategy to increase the successful eradication of H. pylori infections.
Towards a New Concept of Regenerative Endodontics Based on Mesenchymal Stem Cell-Derived Secretomes Products
Authors: Costa Luis A. , Eiro Noemi , Vaca Andrea , Vizoso Francisco J.
Published: December 2022

The teeth, made up of hard and soft tissues, represent complex functioning structures of the oral cavity, which are frequently affected by processes that cause structural damage that can lead to their loss. Currently, replacement therapy such as endodontics or implants, restore structural defects but do not perform any biological function, such as restoring blood and nerve supplies. In the search for alternatives to regenerate the dental pulp, two alternative regenerative endodontic procedures (REP) have been proposed: (I) cell-free REP (based in revascularization and homing induction to remaining dental pulp stem cells (DPSC) and even stem cells from apical papilla (SCAP) and (II) cell-based REP (with exogenous cell transplantation). Regarding the last topic, we show several limitations with these procedures and therefore, we propose a novel regenerative approach in order to revitalize the pulp and thus restore homeostatic functions to the dentin-pulp complex. Due to their multifactorial biological effects, the use of mesenchymal stem cells (MSC)-derived secretome from non-dental sources could be considered as inducers of DPSC and SCAP to completely regenerate the dental pulp. In partial pulp damage, appropriate stimulate DPSC by MSC-derived secretome could contribute to formation and also to restore the vasculature and nerves of the dental pulp.
Radiomics-Based Machine Learning for Predicting the Injury Time of Rib Fractures in Gemstone Spectral Imaging Scans
Authors: Jin Liang , Sun Yingli , Ma Zongjing , Li Ming
Published: December 2022

This retrospective study aimed to predict the injury time of rib fractures in distinguishing fresh (30 days) or old (90 days) rib fractures. We enrolled 111 patients with chest trauma who had been scanned for rib fractures at our hospital between January 2018 and December 2018 using gemstone spectral imaging (GSI). The volume of interest of each broken end of the rib fractures was segmented using calcium-based material decomposition images derived from the GSI scans. The training and testing sets were randomly assigned in a 7:3 ratio. All cases were divided into groups distinguishing the injury time at 30 and 90 days. We constructed radiomics-based models to predict the injury time of rib fractures. The model performance was assessed by the area under the curve (AUC) obtained by the receiver operating characteristic analysis. We included 54 patients with 259 rib fracture segmentations (34 men; mean age, 52 years ± 12.02; and range, 19–72 years). Nine features were excluded by the least absolute shrinkage and selection operator logistic regression to build the radiomics signature. For distinguishing the injury time at 30 days, the Support Vector Machine (SVM) model and human–model collaboration resulted in an accuracy and AUC of 0.85 and 0.871 and 0.91 and 0.912, respectively, and 0.81 and 0.804 and 0.83 and 0.85, respectively, at 90 days in the testing set. The radiomics-based model displayed good accuracy in differentiating between the injury time of rib fractures at 30 and 90 days, and the human–model collaboration generated more accurate outcomes, which may help to add value to clinical practice and distinguish artificial injury in forensic medicine.
Design of Christmas-Tree-like Microfluidic Gradient Generators for Cell-Based Studies
Authors: Wang Yu-Hsun , Ping Chi-Hung , Sun Yung-Shin
Published: December 2022

Microfluidic gradient generators (MGGs) provide a platform for investigating how cells respond to a concentration gradient or different concentrations of a specific chemical. Among these MGGs, those based on Christmas-tree-like structures possess advantages of precise control over the concentration gradient profile. However, in designing these devices, the lengths of channels are often not well considered so that flow rates across downstream outlets may not be uniform. If these outlets are used to culture cells, such non-uniformity will lead to different fluidic shear stresses in these culture chambers. As a result, cells subject to various fluidic stresses may respond differently in aspects of morphology, attachment, alignment and so on. This study reports the rationale for designing Christmas-tree-like MGGs to attain uniform flow rates across all outlets. The simulation results suggest that, to achieve uniform flow rates, the lengths of vertical channels should be as long as possible compared to those of horizontal channels, and modifying the partition of horizontal channels is more effective than elongating the lengths of vertical channels. In addition, PMMA-based microfluidic chips are fabricated to experimentally verify these results. In terms of chemical concentrations, perfect linear gradients are observed in devices with modified horizontal channels. This design rationale will definitely help in constructing optimal MGGs for cell-based applications including chemotherapy, drug resistance and drug screening.
Diazoxide for Neonatal Hyperinsulinemic Hypoglycemia and Pulmonary Hypertension
Authors: Newman-Lindsay Shoshana , Lakshminrusimha Satyan , Sankaran Deepika
Published: December 2022

Hypoglycemia in neonates is associated with long-term neurodevelopmental effects. Hyperinsulinemic hypoglycemia (HH) is the most common cause of persistent hypoglycemia in neonatal intensive care units. Diazoxide is the only medication that is currently recommended for treatment of HH in neonates. However, the use of diazoxide in neonates is associated with pulmonary hypertension as an adverse effect. In this article, we review the literature on the mechanism of action and adverse effects with the use of diazoxide in neonatal hyperinsulinism. We then present a case series of neonates treated with diazoxide in our neonatal intensive care unit over a 5-year period. Among 23 neonates who received diazoxide, 4 developed pulmonary hypertension and 1 died. All infants who developed pulmonary hypertension were born preterm at less than 36 weeks gestation and had pre-existing risk factors for pulmonary hypertension. HH in preterm neonates, with pre-existing pulmonary hypertension or with risk factors for pulmonary hypertension requires thoughtful management.