bluuebunny/crossref_metadata_2025 huggingface dataset:
A Comparison of Parenting Strategies in a Digital Environment: A Systematic Literature Review
Authors: Banić Leonarda , Orehovački Tihomir
Published: April 2024

In the modern digital landscape, parental involvement in shaping children’s internet usage has gained unprecedented importance. This research delves into the evolving trends of parental mediation concerning children’s internet activities. As the digital realm increasingly influences young lives, the role of parents in guiding and safeguarding their children’s online experiences becomes crucial. The study addresses key research questions to explore the strategies parents adopt, the content they restrict, the rules they establish, the potential exposure to inappropriate content, and the impact of parents’ computer literacy on their children’s internet safety. Additionally, the research includes a thematic question that broadens the analysis by incorporating insights from studies not directly answering the primary questions but contributing valuable context and understanding to the digital parenting arena. Building on this, the findings from a systematic literature review, conducted in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines, highlight a shift towards more proactive parental involvement. Incorporating 49 studies from 11 databases, these findings reveal the current trends and methodologies in parental mediation. Active mediation strategies, which involve positive interactions and discussions about online content, are gaining recognition alongside the prevalent restrictive mediation approaches. Parents are proactively forbidding specific internet content, emphasizing safety and privacy concerns. Moreover, the emergence of parents’ computer literacy as a significant factor influencing their children’s online safety underlines the importance of digital proficiency. By shedding light on the contemporary landscape of parental mediation, this study contributes to a deeper understanding of how parents navigate their children’s internet experiences and the challenges they face in ensuring responsible and secure online engagement. The implications of these findings offer valuable insights for both practitioners and researchers, emphasizing the need for active parental involvement and the importance of enhancing parents’ digital proficiency. Despite limitations due to the language and methodological heterogeneity among the included studies, this research paves the way for future investigations into digital parenting practices.
Advances in Nanocarrier Systems for Overcoming Formulation Challenges of Curcumin: Current Insights
Authors: Jacob Shery , Kather Fathima , Morsy Mohamed , Boddu Sai , Attimarad Mahesh , Shah Jigar , Shinu Pottathil , Nair Anroop
Published: April 2024

Curcumin, an organic phenolic molecule that is extracted from the rhizomes of Curcuma longa Linn, has undergone extensive evaluation for its diverse biological activities in both animals and humans. Despite its favorable characteristics, curcumin encounters various formulation challenges and stability issues that can be effectively addressed through the application of nanotechnology. Nano-based techniques specifically focused on enhancing solubility, bioavailability, and therapeutic efficacy while mitigating toxicity, have been explored for curcumin. This review systematically presents information on the improvement of curcumin’s beneficial properties when incorporated, either individually or in conjunction with other drugs, into diverse nanosystems such as liposomes, nanoemulsions, polymeric micelles, dendrimers, polymeric nanoparticles, solid-lipid nanoparticles, and nanostructured lipid carriers. Additionally, the review examines ongoing clinical trials and recently granted patents, offering a thorough overview of the dynamic landscape in curcumin delivery. Researchers are currently exploring nanocarriers with crucial features such as surface modification, substantial loading capacity, biodegradability, compatibility, and autonomous targeting specificity and selectivity. Nevertheless, the utilization of nanocarriers for curcumin delivery is still in its initial phases, with regulatory approval pending and persistent safety concerns surrounding their use.
Hydrophobic and Luminescent Polydimethylsiloxane PDMS-Y2O3:Eu3+ Coating for Power Enhancement and UV Protection of Si Solar Cells
Authors: Goponenko Darya , Zhumanova Kamila , Shamarova Sabina , Yelzhanova Zhuldyz , Ng Annie , Atabaev Timur Sh.
Published: April 2024

Solar cells have been developed as a highly efficient source of alternative energy, collecting photons from sunlight and turning them into electricity. On the other hand, ultraviolet (UV) radiation has a substantial impact on solar cells by damaging their active layers and, as a result, lowering their efficiency. Potential solutions include the blocking of UV light (which can reduce the power output of solar cells) or converting UV photons into visible light using down-conversion optical materials. In this work, we propose a novel hydrophobic coating based on a polydimethylsiloxane (PDMS) layer with embedded red emitting Y2O3:Eu3+ (quantum yield = 78.3%) particles for UV radiation screening and conversion purposes. The favorable features of the PDMS-Y2O3:Eu3+ coating were examined using commercially available polycrystalline silicon solar cells, resulting in a notable increase in the power conversion efficiency (PCE) by ~9.23%. The chemical and UV stability of the developed coatings were assessed by exposing them to various chemical conditions and UV irradiation. It was found that the developed coating can endure tough environmental conditions, making it potentially useful as a UV-protective, water-repellent, and efficiency-enhancing coating for solar cells.
Sensing at the Nanoscale Using Nitrogen-Vacancy Centers in Diamond: A Model for a Quantum Pressure Sensor
Authors: Paudel Hari P. , Lander Gary R. , Crawford Scott E. , Duan Yuhua
Published: April 2024

The sensing of stress under harsh environmental conditions with high resolution has critical importance for a range of applications including earth’s subsurface scanning, geological CO2 storage monitoring, and mineral and resource recovery. Using a first-principles density functional theory (DFT) approach combined with the theoretical modelling of the low-energy Hamiltonian, here, we investigate a novel approach to detect unprecedented levels of pressure by taking advantage of the solid-state electronic spin of nitrogen-vacancy (NV) centers in diamond. We computationally explore the effect of strain on the defect band edges and band gaps by varying the lattice parameters of a diamond supercell hosting a single NV center. A low-energy Hamiltonian is developed that includes the effect of stress on the energy level of a ±1 spin manifold at the ground state. By quantifying the energy level shift and split, we predict pressure sensing of up to 0.3 MPa/Hz using the experimentally measured spin dephasing time. We show the superiority of the quantum sensing approach over traditional optical sensing techniques by discussing our results from DFT and theoretical modelling for the frequency shift per unit pressure. Importantly, we propose a quantum manometer that could be useful to measure earth’s subsurface vibrations as well as for pressure detection and monitoring in high-temperature superconductivity studies and in material sciences. Our results open avenues for the development of a sensing technology with high sensitivity and resolution under extreme pressure limits that potentially has a wider applicability than the existing pressure sensing technologies.
A 12-Week, Single-Centre, Randomised, Double-Blind, Placebo-Controlled, Parallel-Design Clinical Trial for the Evaluation of the Efficacy and Safety of Lactiplantibacillus plantarum SKO-001 in Reducing Body Fat
Authors: Shin Seon Mi , Park Jeong-Su , Kim Sang Back , Cho Young Hee , Seo Hee , Lee Hak Sung
Published: April 2024

There is growing evidence linking gut microbiota to overall health, including obesity risk and associated diseases. Lactiplantibacillus plantarum SKO-001, a probiotic strain isolated from Angelica gigas, has been reported to reduce obesity by controlling the gut microbiome. In this double-blind, randomised clinical trial, we aimed to evaluate the efficacy and safety of SKO-001 in reducing body fat. We included 100 participants randomised into SKO-001 or placebo groups (1:1) for 12 weeks. Dual-energy X-ray absorptiometry was used to objectively evaluate body fat reduction. Body fat percentage (p = 0.016), body fat mass (p = 0.02), low-density lipoprotein-cholesterol levels (p = 0.025), and adiponectin levels (p = 0.023) were lower in the SKO-001 group than in the placebo group after 12 weeks of SKO-001 consumption. In the SKO-001 group, the subcutaneous fat area (p = 0.003), total cholesterol levels (p = 0.003), and leptin levels (p = 0.014) significantly decreased after 12 weeks of SKO-001 consumption compared with baseline values. Additionally, SKO-001 did not cause any severe adverse reactions. In conclusion, SKO-001 is safe and effective for reducing body fat and has the potential for further clinical testing in humans.
Selection of Fermentation Supernatant from Probiotic Strains Exhibiting Intestinal Epithelial Barrier Protective Ability and Evaluation of Their Effects on Colitis Mouse and Weaned Piglet Models
Authors: Abrehame Solomon , Hung Man-Yun , Chen Yu-Yi , Liu Yu-Tse , Chen Yung-Tsung , Liu Fang-Chueh , Lin Yu-Chun , Chen Yen-Po
Published: April 2024

The intestinal epithelial barrier can prevent the invasion of pathogenic microorganisms and food antigens to maintain a consistent intestinal homeostasis. However, an imbalance in this barrier can result in various diseases, such as inflammatory bowel disease, malnutrition, and metabolic disease. Thus, the aim of this study was to select probiotic strains with epithelial barrier-enhancing ability in cell-based model and further investigate them for their improving effects on colitis mouse and weaned piglet models. The results showed that selected specific cell-free fermentation supernatants (CFSs) from Ligilactobacillus salivarius P1, Lactobacillus gasseri P12, and Limosilactobacillus reuteri G7 promoted intestinal epithelial cell growth and proliferation, strengthening the intestinal barrier in an intestinal epithelial cell line Caco-2 model. Further, the administration of CFSs of L. salivarius P1, L. gasseri P12, and L. reuteri G7 were found to ameliorate DSS-induced colitis in mice. Additionally, spray-dried powders of CFS from the three strains were examined in a weaned piglet model, only CFS powder of L. reuteri G7 could ameliorate the feed/gain ratio and serum levels of D-lactate and endotoxin. In conclusion, a new potential probiotic strain, L. reuteri G7, was selected and showed ameliorating effects in both colitis mouse and weaned piglet models.
Characterizing Factors Associated with Excess Body Weight: A Descriptive Study Using Principal Component Analysis in a Population with Overweight and Obesity
Authors: Fernández-Cardero Álvaro , Sierra-Cinos José , López-Jiménez Adrián , Beltrán Beatriz , Cuadrado Carmen , García-Conesa María , Bravo Laura , Sarriá Beatriz
Published: April 2024

Obesity is a worldwide epidemic, making it crucial to understand how it can be effectively prevented/treated. Considering that obesity is a multifactorial condition, this article carried out a baseline cross-sectional study of the variables involved in the disorder. Eighty-four subjects with overweight/obesity were recruited. Dietary baseline information was obtained by analysing three 24 h recalls. Resting metabolic rate was measured using indirect calorimetry, physical activity was measured through accelerometry, cardiometabolic parameters were determined in blood samples and body composition via anthropometry and bioimpedance. A univariant and multivariate exploratory approach was carried out using principal component analysis (PCA). Large inter-individual variability was observed in dietetic, biochemical, and physical activity measurements (coefficient of variation ≥ 30%), but body composition was more uniform. Volunteers had an unbalanced diet and low levels of physical activity. PCA reduced the 26 analysed variables to 4 factors, accounting for 65.4% of the total data variance. The main factor was the “dietetic factor”, responsible for 24.0% of the total variance and mainly related to energy intake, lipids, and saturated fatty acids. The second was the “cardiometabolic factor” (explaining 16.8% of the variability), the third was the “adiposity factor” (15.2%), and the last was the “serum cholesterol factor” (9.4%).
S/O/W Emulsion with CAPE Ameliorates DSS-Induced Colitis by Regulating NF-κB Pathway, Gut Microbiota and Fecal Metabolome in C57BL/6 Mice
Authors: Wei Xuelin , Dai Juan , Liu Ruijia , Wan Guochao , Gu Shiyu , Du Yuwei , Yang Xinyue , Wang Lijun , Huang Yukun , Chen Pengfei , Chen Xianggui , Yang Xiao , Wang Qin
Published: April 2024

Inflammatory bowel disease (IBD) has attracted much attention worldwide due to its prevalence. In this study, the effect of a solid-in-oil-in-water (S/O/W) emulsion with Caffeic acid phenethyl ester (CAPE, a polyphenolic active ingredient in propolis) on dextran sulfate sodium (DSS)-induced colitis in C57BL/6 mice was evaluated. The results showed that CAPE-emulsion could significantly alleviate DSS-induced colitis through its effects on colon length, reduction in the disease activity index (DAI), and colon histopathology. The results of ELISA and Western blot analysis showed that CAPE-emulsion can down-regulate the excessive inflammatory cytokines in colon tissue and inhibit the expression of p65 in the NF-κB pathway. Furthermore, CAPE-emulsion promoted short-chain fatty acids production in DSS-induced colitis mice. High-throughput sequencing results revealed that CAPE-emulsion regulates the imbalance of gut microbiota by enhancing diversity, restoring the abundance of beneficial bacteria (such as Odoribacter), and suppressing the abundance of harmful bacteria (such as Afipia, Sphingomonas). The results of fecal metabolome showed that CAPE-emulsion restored the DSS-induced metabolic disorder by affecting metabolic pathways related to inflammation and cholesterol metabolism. These research results provide a scientific basis for the use of CPAE-emulsions for the development of functional foods for treating IBD.
Renormalisable Non-Local Quark–Gluon Interaction: Mass Gap, Chiral Symmetry Breaking and Scale Invariance
Authors: Chatterjee Arpan , Frasca Marco , Ghoshal Anish , Groote Stefan
Published: April 2024

We derive a Nambu–Jona-Lasinio (NJL) model from a non-local gauge theory and show that it has confining properties at low energies. In particular, we present an extended approach to non-local QCD and a complete revision of the technique of Bender, Milton and Savage applied to non-local theories, providing a set of Dyson–Schwinger equations in differential form. In the local case, we obtain closed-form solutions in the simplest case of the scalar field and extend it to the Yang–Mills field. In general, for non-local theories, we use a perturbative technique and a Fourier series and show how higher-order harmonics are heavily damped due to the presence of the non-local factor. The spectrum of the theory is analysed for the non-local Yang–Mills sector and found to be in agreement with the local results on the lattice in the limit of the non-locality mass parameter running to infinity. In the non-local case, we confine ourselves to a non-locality mass that is sufficiently large compared to the mass scale arising from the integration of the Dyson–Schwinger equations. Such a choice results in good agreement, in the proper limit, with the spectrum of the local theory. We derive a gap equation for the fermions in the theory that gives some indication of quark confinement in the non-local NJL case as well. Confinement seems to be a rather ubiquitous effect that removes some degrees of freedom in the original action, favouring the appearance of new observable states, as seen, e.g., for quantum chromodynamics at lower energies.
FAM19A4 and hsa-miR124-2 Double Methylation as Screening for ASC-H- and CIN1 HPV-Positive Women
Authors: Peronace Cinzia , Cione Erika , Abrego-Guandique Diana Marisol , Fazio Marco De , Panduri Giuseppina , Caroleo Maria Cristina , Cannataro Roberto , Minchella Pasquale
Published: April 2024

The DNA methylation levels of host cell genes increase with the severity of the cervical intraepithelial neoplasia (CIN) grade and are very high in cervical cancer. Our study aims to evaluate FAM19A4 and hsa-miR124-2 methylation in Atypical Squamous cells with high-grade squamous intraepithelial lesions (ASC-H) and in CIN1, defined as low-grade squamous intraepithelial lesions (LSILs) by the Bethesda classification, as possible early warning biomarkers for managing women with high-risk HPV infections (hrHPV). FAM19A4 and hsa-miR124-2 methylation tests were conducted on fifty-six cervical screening samples from a subset of women aged 30–64 years old. Specimens were collected into ThinPrep PreservCyt Solution. Their HrHPV genotype and cytology diagnosis were known. A Qiasure (Qiagen) was used for FAM19A4 and hsa-miR124-2 methylation testing on bisulfite-converted DNA, according to the manufacturer’s specifications. The reported results were hypermethylation-positive or -negative. We found that FAM194A4 and hsa-miR124-2 methylation was detected in 75% of ASC-H cases with a persistent infection of hrHPV. A total of 60% of CIN1 lesions were found to be positive for methylation, and 83.3% were when the cytology was CIN2/3. In addition, as a novelty of this pilot study, we found that combined FAM19A4 and hsa-miR124-2 methylation positivity rates (both methylated) were associated with the HPV genotypes 16, 18, and 59 and covered 22 and 25% of ASC-H and CIN1 cases, respectively. The methylation of these two genes, in combination with HPV genotyping, can be used as an early warning biomarker in the management and follow-up of women with ASC-H and CIN1 to avoid their progression to cervical cancer.
Assessment of the Risk Impact of SARS-CoV-2 Infection Prevalence between Cats and Dogs in America and Europe: A Systematic Review and Meta-Analysis
Authors: Silva Marcos Jessé Abrahão , Santana Davi Silva , Lima Marceli Batista Martins , Silva Caroliny Soares , de Oliveira Letícia Gomes , Monteiro Ellerson Oliveira Loureiro , Dias Rafael dos Santos , Pereira Bruna de Kássia Barbosa , Nery Paula Andresa da Silva , Ferreira Márcio André Silva , Sarmento Matheus Alonso de Souza , Ayin Andrea Alexandra Narro , Mendes de Oliveira Ana Cristina , Lima Karla Valéria Batista , Lima Luana Nepomuceno Gondim Costa
Published: April 2024

The COVID-19 pandemic represented a huge obstacle for public health and demonstrated weaknesses in surveillance and health promotion systems around the world. Its etiological agent, SARS-CoV-2, of zoonotic origin, has been the target of several studies related to the control and prevention of outbreaks and epidemics of COVID-19 not only for humans but also for animals. Domestic animals, such as dogs and cats, have extensive contact with humans and can acquire the infection both naturally and directly from humans. The objective of this article was to summarize the seroprevalence findings of SARS-CoV-2 in dogs and cats and correlate them with the strength of infection risk between each of them. This is a systematic review and meta-analysis following the recommendations of PRISMA 2020. The search and selection of papers was carried out using in vivo experimental works with animals using the descriptors (MeSH/DeCS) “Animal”, “Public Health”, “SARS-CoV-2” and “Pandemic” (together with AND) in English, Portuguese or Spanish for Science Direct, PUBMED, LILACS and SciELO databases. The ARRIVE checklist was used for methodological evaluation and the Comprehensive Meta-Analysis v2.2 software with the Difference Risk (RD) test to evaluate statistical inferences (with subgroups by continent). Cats showed greater susceptibility to SARS-CoV-2 compared to dogs both in a joint analysis of studies (RD = 0.017; 95% CI = 0.008–0.025; p < 0.0001) and in the American subgroup (RD = 0.053; 95% CI = 0.032–0.073; p < 0.0001), unlike the lack of significant difference on the European continent (RD = 0.009; 95% CI = −0.001–0.018; p = 0.066). Therefore, it was observed that cats have a greater interest in health surveillance due to the set of biological and ecological aspects of these animals, but also that there are a set of factors that can influence the spread and possible spillover events of the virus thanks to the anthropozoonotic context.
Discovery of Small Molecule Glycolytic Stimulants for Enhanced ApoE Lipidation in Alzheimer’s Disease Cell Model
Authors: Patil Sachin P. , Kuehn Bella R.
Published: April 2024

Alzheimer’s disease (AD) is a progressive neurodegenerative disease characterized by pathophysiological deposits of extracellular amyloid beta (Aβ) peptides and intracellular neurofibrillary tangles of tau. The central role of Aβ in AD pathology is well-established, with its increased deposition attributed mainly to its decreased cerebral clearance. Here, it is noteworthy that apolipoprotein E (ApoE), the most significant risk factor for AD, has been shown to play an isoform-specific role in clearing Aβ deposits (ApoE2 > ApoE3 > ApoE4), owing mainly to its lipidation status. In addition to the pathophysiological Aβ deposits, AD is also characterized by abnormal glucose metabolism, which is a distinct event preceding Aβ deposition. The present study established, for the first time, a possible link between these two major AD etiologies, with glucose metabolism directly influencing ApoE lipidation and its secretion by astrocytes expressing human ApoE4. Specifically, glucose dose-dependently activated liver X receptor (LXR), leading to elevated ABCA1 and ABCG1 protein levels and enhanced ApoE lipidation. Moreover, co-treatment with a glycolytic inhibitor significantly inhibited this LXR activation and subsequent ApoE lipidation, further supporting a central role of glucose metabolism in LXR activation leading to enhanced ApoE lipidation, which may help against AD through potential Aβ clearance. Therefore, we hypothesized that pharmacological agents that can target cellular energy metabolism, specifically aerobic glycolysis, may hold significant therapeutic potential against AD. In this context, the present study also led to the discovery of novel, small-molecule stimulants of astrocytic glucose metabolism, leading to significantly enhanced lipidation status of ApoE4 in astrocytic cells. Three such newly discovered compounds (lonidamine, phenformin, and berberine), owing to their promising cellular effect on the glycolysis-ApoE nexus, warrant further investigation in suitable in vivo models of AD.
Preventive and Therapeutic Effects of Crocetin in Rats with Heart Failure
Authors: Ma Renqiang , Li Sijia , Mo Qingmei , Chen Xiaojuan , Liang Yan , Hu Tao , Hu Hui , He Bao , Li Renshi , Kou Junping , Yu Boyang
Published: April 2024

Gardenia is both a food and medicine plant. It is widely used for cardiovascular protection, and its main bioactive ingredient is crocetin. This study aims to observe the therapeutic effects of crocetin on chronic heart failure in rats induced by various etiologies. It further compares the efficacy differences between preventative and treatment administration, varying dosages, and treatment durations, to provide improved guidance for medication in heart failure rats and determine which categories of chronic heart failure rats might benefit most from crocetin. Chronic heart failure models induced by abdominal aorta constriction, renal hypertension, and coronary artery ligation were constructed. By examining cardiac function, blood biochemistry, and histopathology, the study assessed the preventive and therapeutic effects of crocetin on load-induced and myocardial ischemia-induced heart failure. The results showed that in all three models, both treatment and preventative administration of crocetin significantly improved chronic heart failure in rats, especially in preventative administration. The results indicate crocetin may be beneficial for improving symptoms and functional capacity in rats with heart failure. Furthermore, long-term administration was more effective than short-term administration across all three rat models, with therapeutic onset observed over 6 weeks.
Molecular Ballet: Investigating the Complex Interaction between Self-Assembling Dendrimers and Human Serum Albumin via Computational and Experimental Methods
Authors: Cavalieri Gabriele , Marson Domenico , Giurgevich Nicoletta , Valeri Rachele , Felluga Fulvia , Laurini Erik , Pricl Sabrina
Published: April 2024

Dendrimers, intricate macromolecules with highly branched nanostructures, offer unique attributes including precise control over size, shape, and functionality, making them promising candidates for a wide range of biomedical applications. The exploration of their interaction with biological environments, particularly human serum albumin (HSA), holds significant importance for biomedical utilization. In this study, the interaction between HSA and a recently developed self-assembling amphiphilic dendrimer (AD) was investigated using various experimental techniques. Fluorescence spectroscopy and isothermal titration calorimetry revealed moderate interactions between the protein and the AD nanomicelles (NMs), primarily attributed to favorable enthalpic contributions arising from electrostatic interactions and hydrogen bonding. Structural analysis indicated minimal changes in HSA upon complexation with the AD NMs, which was further supported by computational simulations demonstrating stable interactions at the atomistic level. These findings provide valuable insights into the binding mechanisms and thermodynamic parameters governing HSA/AD NM interactions, thereby contributing to the understanding of their potential biomedical applications.
The Politics of Film Aesthetics: Filmososphy, Post-Theory, and Rancière
Authors: Koutras Konstantinos
Published: April 2024

The question of aesthetics in film-theoretical discourse today is split between, on the one hand, a film-phenomenological or “filmosophical” approach that values the putatively immanent relation between film and the mind and, on the other, the naturalizing epistemology of post-theory, which reduces the question of film aesthetics to one of poetics. What unites these otherwise disparate projects is the consideration of aesthetics divorced from the question of politics; in both cases, the social or political significance of the film–spectator relationship has been summarily purged. In this article, I will offer an alternative account of film aesthetics that draws on Jacques Rancière’s theory concerning the mutually determining relationship between aesthetics and politics. In particular, I will consider the relevance of Rancière’s thesis concerning what he calls the distribution of sensible to current accounts, as well as taking up his novel consideration of aesthetic distance and the “emancipated” spectator. With respect to film phenomenology, I will examine how its film-theoretical program rests on the flawed concept of a de-politicized spectator enchained by the film image. With respect to post-theory, I will examine how its appropriation from cognitive science of the rational agent model of meaning making inappropriately limits the political potential of film aesthetics.
Microwave Photonics Broadband Doppler Velocity Simulator with High Spurious Suppression Ratio by Using Serrodyne Modulation
Authors: Liu Zhe , Wang Dayong , Zhu Weimin , Zhang Jing , Wang Yunxin , Yao Jinchuan , Zhang Yu
Published: April 2024

A Doppler velocity simulation method based on serrodyne modulation is proposed to achieve the frequency shift from hundred hertz to megahertz. One sub-phase modulation (PM) in a dual-parallel dual-drive Mach–Zehnder modulator loads a sawtooth signal to achieve a small frequency shift of the optical carrier. The other three sub-PMs implement carrier-suppressed double-band modulation of the RF signal. The RF signal is directly coupled from the receiving antenna to the modulator’s RF port without any electrical devices like a 90° hybrid, which ensures a broad operational bandwidth of the system. After filtering out one of the RF modulation sidebands by an optical filter, Doppler frequency shifting (DFS) is realized through frequency beating. The half-wave voltage of modulators rapidly decreases at low frequency shifts, leading to an increase in spurious signals. In order to improve the spurious suppression ratio (SSR) of DFS, a digital pre-distortion compensation based on the measured half-wave voltage is implemented in the frequency domain. Experimental results show that SSRs are larger than 35 dB when frequency shifts range from 0.1 kHz to 1 MHz. The RF operation bandwidth covers 2–40 GHz. The effectiveness of a Doppler velocity simulator is evaluated, and the simulation velocity error is less than 0.06 km/h. The proposed method has potential applications in both broadband electronic warfare and traffic metering applications.
Photoluminescence Enhancement and Carrier Dynamics of Charged Biexciton in Monolayer WS2 Coupled with Plasmonic Nanocavity
Authors: Geng Huiqiang , Liu Qirui , Tang Yuxiang , Wei Ke
Published: April 2024

Monolayer two-dimensional transition metal dichalcogenide (TMD)-based materials have become one of the ideal platforms for the study of multibody interactions due to their rich excitonic complexes. The coupling between optical nanocavity and material has become an important means for manipulating the optical properties of materials, but there are few studies on the coupling of nanocavities and the multi-body effect in materials. In this study, we investigate the optical properties of silver nanodisk (Ag ND) arrays covering a monolayer WS2. In the experimental sample, we observed a ~114.3-fold photoluminescence enhancement of charged biexciton in the heterostructure region, as compared to the monolayer WS2 region, a value which is much higher than those for exciton (~2.2-fold) and trion (~16.4-fold), a finding which is attributed to the Fano resonant coupling between monolayer WS2 and the Ag ND. By means of time-resolved spectroscopy, we studied the carrier dynamics in the hybrid system. Our findings reveal that resonant coupling promotes the formation and radiation recombination processes of the charged biexciton, significantly reducing the radiative recombination lifetime by ~15-fold, which is much higher than the measurement in exciton (~2-fold). Our results provide an opportunity to understand the multibody physics of coupling with nanocavities, which could facilitate the application of multi-body excitons in the fields of light-emitting devices and lasers, etc.
Role of N1-Domain, Linker, N2-Domain, and Latch in the Binding Activity and Stability of the Collagen-Binding Domain for the Collagen-Binding Protein Cbm from Streptococcus mutans
Authors: Nishi Akari , Hirata Azumi , Mukaiyama Atsushi , Tanaka Shun-ichi , Nomura Ryota , Nakano Kazuhiko , Takano Kazufumi
Published: April 2024

A special type of Streptococcus mutans expressing collagen-binding proteins (CBPs), Cnm, and Cbm, on the cell surface has been shown to be highly pathogenic. It is believed that S. mutans with CBPs that has entered the blood vessel attaches to collagen molecules exposed from the damaged blood vessel, inhibiting aggregation by platelets and increasing bleeding. Therefore, it is crucial to understand the molecular characteristic features of CBPs to protect against and cure S. mutans-related diseases. In this work, we highlighted the Cbm/collagen-binding domain (CBD) and examined its binding ability and thermal stability using its domain/region exchange variants in more detail. The CBD comprises the N1-domain, a linker, N2-domain, and a latch (N1–N2~), where the latch interacts with the N1-domain to form a β-sheet. The collagen-binding activity of the Cbm/CBD domain/region exchange variants was investigated using ELISA. Binding assays demonstrated that the N-domain_linker_N-domain composition is necessary for collagen binding as previously reported, newly that the latch is involved in binding through the β-sheet with the N1-domain when the N1-domain is present at the N-terminal position, and that the N2-domain is particularly important for collagen binding at both the N- and C-terminal positions. Thermal denaturation experiments newly revealed that the linker and latch bound to the N-domain contribute to N-domain stabilization but have no effect on the N-domain_linker_N-domain molecule, which contains two N-domains. It has also been shown that the N-terminal N2-domain destabilizes the N-domain_linker_N-domain structure. The results of this study will contribute to the rapid detection of CBP, development of CBP-targeted therapies, and application of CBPs to protein engineering using their collagen-binding ability.
OsSCYL2 is Involved in Regulating ABA Signaling-Mediated Seed Germination in Rice
Authors: Xu Minyan , Zhang Wei , Jiao Yuhuan , Yang Qing , Chen Meng , Cheng Hu , Cheng Beijiu , Zhang Xin
Published: April 2024

Seed germination represents a multifaceted biological process influenced by various intrinsic and extrinsic factors. In the present study, our investigation unveiled the regulatory role of OsSCYL2, a gene identified as a facilitator of seed germination in rice. Notably, the germination kinetics of OsSCYL2-overexpressing seeds surpassed those of their wild-type counterparts, indicating the potency of OsSCYL2 in enhancing this developmental process. Moreover, qRT-PCR results showed that OsSCYL2 was consistently expressed throughout the germination process in rice. Exogenous application of ABA on seeds and seedlings underscored the sensitivity of OsSCYL2 to ABA during both seed germination initiation and post-germination growth phases. Transcriptomic profiling following OsSCYL2 overexpression revealed profound alterations in metabolic pathways, MAPK signaling cascades, and phytohormone-mediated signal transduction pathways, with 15 genes related to the ABA pathways exhibiting significant expression changes. Complementary in vivo and in vitro assays unveiled the physical interaction between OsSCYL2 and TOR, thereby implicating OsSCYL2 in the negative modulation of ABA-responsive genes and its consequential impact on seed germination dynamics. This study elucidated novel insights into the function of OsSCYL2 in regulating the germination process of rice seeds through the modulation of ABA signaling pathways, thereby enhancing the understanding of the functional significance of the SCYL protein family in plant physiological processes.
A High-Proton Conductivity All-Biomass Proton Exchange Membrane Enabled by Adenine and Thymine Modified Cellulose Nanofibers
Authors: Xie Chong , Yang Runde , Wan Xing , Li Haorong , Ge Liangyao , Li Xiaofeng , Zhao Guanglei
Published: April 2024

Nanocellulose fiber materials were considered promising biomaterials due to their excellent biodegradability, biocompatibility, high hydrophilicity, and cost-effectiveness. However, their low proton conductivity significantly limited their application as proton exchange membranes. The methods previously reported to increase their proton conductivity often introduced non-biodegradable groups and compounds, which resulted in the loss of the basic advantages of this natural polymer in terms of biodegradability. In this work, a green and sustainable strategy was developed to prepare cellulose-based proton exchange membranes that could simultaneously meet sustainability and high-performance criteria. Adenine and thymine were introduced onto the surface of tempo-oxidized nanocellulose fibers (TOCNF) to provide many transition sites for proton conduction. Once modified, the proton conductivity of the TOCNF membrane increased by 31.2 times compared to the original membrane, with a specific surface area that had risen from 6.1 m²/g to 86.5 m²/g. The wet strength also increased. This study paved a new path for the preparation of environmentally friendly membrane materials that could replace the commonly used non-degradable ones, highlighting the potential of nanocellulose fiber membrane materials in sustainable applications such as fuel cells, supercapacitors, and solid-state batteries.
In Vivo Evaluation of Innovative Gadolinium-Based Contrast Agents Designed for Bioimaging Applications
Authors: Voicu Sorina Nicoleta , Gheran Cecilia Virginia , Balta Cornel , Hermenean Anca , Callewaert Maité , Chuburu Françoise , Dinischiotu Anca
Published: April 2024

The aim of this study was the investigation of biochemical and histological changes induced in different tissues, as a result of the subcutaneous administration of Gd nanohydrogels (GdDOTA⸦CS-TPP/HA) in a CD-1 mouse strain. The nanohydrogels were obtained by encapsulating contrast agents (GdDOTA) in a biocompatible polymer matrix composed of chitosan (CS) and hyaluronic acid (HA) through the ionic gelation process. The effects of Gd nanohydrogels on the redox status were evaluated by measuring specific activities of the antioxidant enzymes catalase (CAT), glutathione peroxidase (GPx), and superoxide dismutase (SOD), as well as oxidative stress markers, such as reduced glutathione (GSH), malondialdehyde (MDA), advanced oxidation protein products (AOPP), and protein-reactive carbonyl groups (PRCG), in the liver, kidney, and heart tissues. The nitrosylated proteins expression were analyzed with Western Blot and the serum biochemical markers were measured with spectrophotometric methods. Also, a histological analysis of CD-1 mouse tissues was investigated. These results indicated that Gd nanohydrogels could potentially be an alternative to current MRI contrast agents thanks to their low toxicity in vivo.
Bioinspired Fabrication of an Insensitive Ammonium Perchlorate Core–Shell Composite with Polydopamine Coating
Authors: Huang Yafeng , Tian Xuan , Wang Jinfei , Zhong Kejun , Chen Yuan , Li Chenglong , Jia Pengxiang
Published: April 2024

In this research, an ammonium perchlorate/polydopamine (AP/PDA) core–shell composite was prepared in a non-aqueous solution to reduce the mechanical sensitivity of ammonium perchlorate (AP). The result showed that the AP/PDA core–shell composite could be successfully constructed in ethyl acetate solution with an AP recovery rate that reached 86%. The mechanical sensitivity of the obtained AP/PDA core–shell composite was significantly reduced with a PDA content of only 0.76%. The DSC and TG also indicated that the coating of PDA showed catalytic activity in the thermal decomposition of AP with a lower decomposition temperature and a decreased Ea value of AP. Thus, this study proposed a simple strategy for achieving a good balanced between harnessing the energy and ensuring the safety of ammonium perchlorate by significantly reducing its mechanical sensitivity by using a very low polydopamine coating layer content, and this shows great potential for the design and fabrication of insensitive energetic composites for use in propellants.
The Effect of Notch and Molecular Weight on the Impact Fracture Behavior of Polycarbonate
Authors: Xu Xueting , Wang Tao , Sun Qiwei , Wang Bolun , Ge Yong , Lang Jianlin , Yan Yue
Published: April 2024

The impact protection applications of polycarbonate (PC) products are gradually increasing. Due to the high sensitivity of PC to notches, research on notch impacts has become very important. In this paper, the impact performance of PC with two different molecular weights under different notch states was investigated. Three notch size factors, namely notch tip radius, notch angle, and notch center depth, were selected to design orthogonal experiments and research impact toughness. Subsequently, a single-factor study was conducted on the impact radius at the tip of the notch, which was the most important factor affecting the impact performance. Research shows that the brittle–ductile-transition tip radius of high-molecular-weight PC is 0.15 mm, and it has a higher impact toughness than low-molecular-weight PC during the brittle fracture process. The brittle–ductile-transition tip radius of lower molecular weight is 0.25 mm, while low-molecular-weight PC has a higher impact toughness during the ductile fracture process. The brittle and ductile fracture mechanisms of PC with different molecular weights were analyzed by observing the stress changes and cross-sectional morphology.
A Screen-Printed Voltammetric Sensor Modified with Electropolymerized Molecularly Imprinted Polymer (eMIP) to Determine Gallic Acid in Non-Alcoholic and Alcoholic Beverages
Authors: Zanoni Camilla , Dallù Lucrezia Virginia , Costa Clementina , Cutaia Alessandra , Alberti Giancarla
Published: April 2024

This paper presents a low-cost disposable sensor for gallic acid (GA) detection in non-alcoholic and alcoholic beverages using a screen-printed cell (SPC) whose working electrode (in graphite) is modified with electrosynthesized molecularly imprinted polypyrrole (eMIP). Our preliminary characterization of the electrochemical process shows that gallic acid (GA) undergoes irreversible oxidation at potentials of about +0.3 V. The peak potential is not affected by the presence of the eMIP film and alcohol percentages (ethanol) up to 20%. The GA determination is based on a differential pulse voltammetry (DPV) analysis leveraging its oxidation peak. The calibration data and the figures of merit of the analytical method (LOD, LOQ, and linear range) are calculated. To validate the feasibility of the sensor’s application for the dosing of GA in real matrices, some non-alcoholic and alcoholic beverages are analyzed. The results are then compared with those reported in the literature and with the total polyphenol content determined by the Folin–Ciocalteu method. In all cases, the concentrations of GA align with those previously found in the literature for the beverages examined. Notably, the values are consistently lower than the total polyphenol content, demonstrating the sensor’s selectivity in discriminating the target molecule from other polyphenols present.
Preparation of Environmentally Friendly Oil- and Water-Resistant Paper Using Holo-Lignocellulosic Nanofibril (LCNF)-Based Composite Coating
Authors: Wang Shengdan , Pei Lihua , Wei Jichao , Xie Jiabao , Ji Xingxiang , Wang Yukang , Jia Peng , Jiao Yajuan
Published: April 2024

In the present study, an environmentally friendly oil- and water-resistant paper was developed using a holo-lignocellulosic nanofibril (LCNF)-based composite coating. The LCNF was prepared from wheat straw using a biomechanical method. Characterizations of oil- and water-resistant coated paper and the effect of LCNF content on the performance of the coated paper were confirmed by combining contact angle analysis, Cobb 300s, and mechanical performance tests. The results show that the barrier performance and mechanical strength of the coated paper were greatly improved with the increase of LCNF content. The contact angle of oil and water of coated paper containing 50% LCNF were 69° and 78°, respectively, while the contact angle of oil and water of the base paper were only 30° and 20°, respectively. Cobb 300s values reduced from 110 g/m2 to 30 g/m2 when the LCNF content increased from 50% to 90%. Moreover, under the coating amount of 20 g/m2, the tensile strength of the coating paper was 0.980 KN/m, an increase of 10.11% compared with the base paper. The bursting strength reached 701.930 KPa, which was 10.75% higher than the base paper. In short, it is feasible to prepare LCNF from wheat straw, and apply it to produce water-proof and oil-proof paper. The water-proof and oil-proof paper developed in this study not only offers a novel approach to addressing white pollution but also presents a new research avenue for exploring the potential applications of agricultural waste.
Self-Assembly Study of Block Copolypeptoids in Response to pH and Temperature Stimulation
Authors: Liu Di , Yang Kang , Xu Liugen , Shen Xiran , Feng Lei , Jiang Yangang , Ali Amjad , Lu Jianwei , Guo Li
Published: April 2024

Polypeptoids with well-designed structures have the ability to self-assemble into nanomaterials, which have wide potential applications. In this study, a series of diblock copolypeptoids were synthesized via ring-opening polymerization followed by click chemistry and exhibited both temperature and pH stimulation responsiveness. Under specific temperature and pH conditions, the responsive blocks in the copolypeptoids became hydrophobic and aggregated to form micelles. The self-assembly process was monitored using the UV-Vis and DLS methods, which suggested the reversible transition of free molecules to micelles and bigger aggregates upon instituting temperature and pH changes. By altering the length and proportion of each block, the copolypeptoids displayed varying self-assembly characteristics, and the transition temperature could be tuned. With good biocompatibility, stability, and no cytotoxicity, the polypeptoids reported in this study are expected to be applied as bionanomaterials in fields including drug delivery, tissue engineering, and intelligent biosensing.
Copper Quantum Dot/Polyacrylamide Composite Nanospheres: Spreading on Quartz Flake Surfaces and Displacing Crude Oil in Microchannel Chips
Authors: Ma Xinru , Yang Haien , Liu Xiaofei , Zeng Lixiang , Li Xinzi , Zheng Lijun , Yang Yu , Cao Lei , Meng Weikai , Zheng Junping
Published: April 2024

Polyacrylamide, silica, and other nanoparticles have all been realized in the field of enhanced oil recovery. Researchers often explore the mechanisms of spreading behavior and simulated displacement to develop more efficient types of nanoparticles. In this study, copper quantum dots were introduced into a acrylamide copolymerization system to obtain composite nanospheres and its structure, topographic, and application performance were characterized. The results show that the composite nanospheres have a particle size of around 25 nm, are uniformly loaded with copper particles, and have good temperature resistance. The spreading ability on the quartz flake surfaces and displacement effect in microchannels of composite nanospheres, acrylamide copolymer nanospheres, and copper quantum dots were compared by nanofluid spreading experiments and microchannel chip oil displacement experiments. The results indicate that the composite nanospheres can effectively reduce the water contact angle, promote the spreading of aqueous phase, and accelerate the oil droplet removal process; the accelerating effect is stronger than other samples. Its oil displacement effect is also the strongest, and it is minimized by the influence of channel size, temperature, and dispersing medium, with better stratigraphic adaptability. This work supports the practical application of copper quantum dot/polyacrylamide composite nanospheres in the oilfield.
Treatise of the Golden Lion: An Exploration of the Doctrine of the Infinite Dependent Arising of Dharmadhātu
Authors: Xiong Ye
Published: April 2024

Among the texts of Huayan Buddhism, the study of the Treatise of the Golden Lion remains at the level of literal translation. Neither the core doctrines of the Treatise nor the original contexts of its delivery have received much scholarly attention. This paper first contextualizes the preaching background of the Treatise and its relevant doctrines, and then conducts a section-by-section explanation of the Treatise with special consideration given to the intention of Fazang and his manner of preaching. The doctrines of the ten mysterious gates and the six characteristics, along with the manifestation of the distinctive teaching of one vehicle, constitute the key components of the preaching. They are the representation and revelation of the doctrine of the infinite dependent arising of dharmadhātu.