bluuebunny/crossref_metadata_2025 huggingface dataset:
Mass Spectrometry Rearrangement Ions and Metabolic Pathway-Based Discovery of Indole Derivatives during the Aging Process in Citrus reticulata ‘Chachi’
Authors: Li Tian , Chen Ke , Wang Xiaoming , Wang Ying , Su Yue , Guo Yinlong
Published: December 2023

The rapid analysis and characterization of compounds using mass spectrometry (MS) may overlook trace compounds. Although targeted analysis methods can significantly improve detection sensitivity, it is hard to discover novel scaffold compounds in the trace. This study developed a strategy for discovering trace compounds in the aging process of traditional Chinese medicine based on MS fragmentation and known metabolic pathways. Specifically, we found that the characteristic component of C. reticulata ‘Chachi’, methyl N-methyl anthranilate (MMA), fragmented in electrospray ionization coupled with collision-induced dissociation (CID) to produce the rearrangement ion 3-hydroxyindole, which was proven to exist in trace amounts in C. reticulata ‘Chachi’ based on comparison with the reference substance using liquid chromatography–tandem mass spectrometry (LC–MS/MS). Combining the known metabolic pathways of 3-hydroxyindole and the possible methylation reactions that may occur during aging, a total of 10 possible indole derivatives were untargeted predicted. These compounds were confirmed to originate from MMA using purchased or synthesized reference substances, all of which were detected in C. reticulata ‘Chachi’ through LC–MS/MS, achieving trace compound analysis from untargeted to targeted. These results may contribute to explaining the aging mechanism of C. reticulata ‘Chachi’, and the strategy of using the CID-induced special rearrangement ion-binding metabolic pathway has potential application value for discovering trace compounds.
Valorization and Utilization of Food Wastes and By-Products: Recent Trends, Innovative Technologies and Sustainability Challenges
Authors: Betoret Noelia , Betoret Ester , Glicerina Virginia Teresa
Published: December 2023

The recovery of food by-products and waste is an issue of universal concern, as every year the food industry generates a huge amount of waste and by-products from a variety of sources [...]
Combining AI Tools with Non-Destructive Technologies for Crop-Based Food Safety: A Comprehensive Review
Authors: Raki Hind , Aalaila Yahya , Taktour Ayoub , Peluffo-Ordóñez Diego H.
Published: December 2023

On a global scale, food safety and security aspects entail consideration throughout the farm-to-fork continuum, considering food’s supply chain. Generally, the agrifood system is a multiplex network of interconnected features and processes, with a hard predictive rate, where maintaining the food’s safety is an indispensable element and is part of the Sustainable Development Goals (SDGs). It has led the scientific community to develop advanced applied analytical methods, such as machine learning (ML) and deep learning (DL) techniques applied for assessing foodborne diseases. The main objective of this paper is to contribute to the development of the consensus version of ongoing research about the application of Artificial Intelligence (AI) tools in the domain of food-crop safety from an analytical point of view. Writing a comprehensive review for a more specific topic can also be challenging, especially when searching within the literature. To our knowledge, this review is the first to address this issue. This work consisted of conducting a unique and exhaustive study of the literature, using our TriScope Keywords-based Synthesis methodology. All available literature related to our topic was investigated according to our criteria of inclusion and exclusion. The final count of data papers was subject to deep reading and analysis to extract the necessary information to answer our research questions. Although many studies have been conducted, limited attention has been paid to outlining the applications of AI tools combined with analytical strategies for crop-based food safety specifically.
Extension of the Optimal Auxiliary Function Method to Solve the System of a Fractional-Order Whitham–Broer–Kaup Equation
Authors: Alsheekhhussain Zainab , Moaddy Khaled , Shah Rasool , Alshammari Saleh , Alshammari Mohammad , Al-Sawalha M. Mossa , Alderremy Aisha Abdullah
Published: December 2023

In this paper, we introduce and implement the optimal auxiliary function method to solve a system of fractional-order Whitham–Broer–Kaup equations, a class of nonlinear partial differential equations with broad applications in mathematical physics. This method provides a systematic and efficient approach to finding accurate solutions for complex systems of fractional-order equations. We give a full analysis using tables and figures to demonstrate the reliability and accuracy of our approach. We confirm the effectiveness of our suggested method in solving the considered equations using numerical simulations and comparisons, emphasizing its potential for applications in a variety of scientific and engineering areas.
Cross-Sectional Study of the Professional Quality of Life of Palliative Care Professionals during the COVID-19 Pandemic
Authors: Campos i Arnal Adrià , Galiana Laura , Sánchez-Ruiz Javier , Sansó Noemí
Published: December 2023

Background: The display of compassionate care by palliative care professionals is of the utmost importance to the patients, their families, and even to their own professional well-being. Lately and, especially due to the emergence of the COVID-19 pandemic, palliative care professionals have been subjected to greater pressures stemming from their work environment, organizational standpoint, and emotional sense of view. Not only have these factors made it harder for professionals to deliver compassionate care to their patients, but they have also decreased their overall well-being. The aim is to study how sociodemographics, workplace characteristics, internal resources, and the COVID-19 pandemic-derived pressures have affected the professionals’ capacity to perform compassionate care and their well-being while at the same time exploring the relationship between compassionate care and well-being. Methods: This study used a cross-sectional design with data gathered from Spanish palliative care professionals. The final sample was formed by 241 participants. They were surveyed about compassion, professional quality of life, well-being, sociodemographic data, working conditions, self-care, and coping with death competence, and the impact of the COVID-19 pandemic. The analyses used were descriptive statistics, bivariate tests, and the construction of a structural equation model. Results: Compassion was predicted by the ability to control their workload and the ability to cope with death. Burnout was predicted by age, workload, workload control, self-care, material resources, and changes in teamwork. Moreover, compassion, age, workload control, and changes in teamwork and self-care were shown to significantly predict compassion satisfaction. When it comes to compassion fatigue, different variables were shown to predict it, those being compassion, control over the workload, social self-care, and the ability to cope with death. Conclusions: Having a healthy lifestyle and an adequate social support system is key to maintaining professional well-being in the case of palliative care professionals. Inner resources such as the ability to perform self-care and the capacity to cope with death are of vital importance to taking care of these professionals. Thus, it would be beneficial to establish training programs focused on these aspects in the myriad of sanitary centers that perform these tasks, as these abilities are necessary to withstand the work-related pressures and, at the same time, be able to provide compassionate care for patients.
Micropropagation and Acclimatization of Monstera deliciosa Liebm. ‘Thai Constellation’
Authors: Jing Yifan , Beleski David , Vendrame Wagner
Published: December 2023

Monstera deliciosa Liebm. ‘Thai Constellation’ is a variegated variety of M. deliciosa belonging to the family Araceae, which has become a new favorite in the foliage plant market. However, limited studies exist on its propagation, and growers have difficulties in achieving large-scale production. This study aimed at developing an efficient protocol for the micropropagation of M. deliciosa using SETIS™ temporary immersion bioreactors. Furthermore, we aimed at evaluating the role of a novel biostimulant (IQ Forte) in the acclimatization of M. deliciosa. Significant differences were observed among the different treatments, showing higher multiplication rates under TIS conditions as compared to the semi-solid control. Adjusting immersion parameters also showed benefits in improving multiplication rates. The novel biostimulant (IQ Forte) did not provide significant gains in growth of M. deliciosa ‘Thai Constellation’ during acclimatization.
Compatibility and Possibility of New Ornamental Geophytes for Their Utilization in Landscape Architecture
Authors: Babarabie Mehrdad , Sardoei Ali Salehi , Jamali Babak , Hatami Mehrnaz , Nicola Silvana , Devecchi Marco
Published: December 2023

Ornamental geophytes, renowned for their beauty, hold a special place among flower enthusiasts and producers, enhancing the aesthetic appeal of gardens and orchards. The main aim of this study was to ascertain the viability of cultivating decay-resistant genotypes and identify appropriate planting locations for each species within a one-to-three-year timeframe, contingent upon the specific species. The research took place at the Flower and Plant Production Center of the Gorgan Municipality’s Landscape and Urban Green Space Organization in Iran, with the primary focus on leveraging various geophyte flower species to optimize urban landscapes and elevate their visual allure. Utilizing a completely randomized block design with three replications, the study examined numerous species in the landscape. Various plant growth parameters were evaluated, including flowering time, optimal planting time, flower longevity on the plants, speed of underground bulb sprouting time, and visual quality of the samples. Results revealed that Narcissus jonquilla and Alstroemeria aurea cv. Balance exhibited the longest flower longevity, lasting for 43 days in the second year of growth. Conversely, Gladiolus hybrida (cv. Alexander) and Canna indica (cv. Flaccida and cv. Phasion) demonstrated a flower longevity of 13 days across both cultivation years. Alstroemeria and Crocosmia showed the shortest flowering time, significantly reduced compared to the first year due to the altered planting time. The assessment of visual quality highlighted Polianthes, Dahlia, and Gladiolus cultivars as displaying the highest visual appeal among the studied species. These findings yield valuable insights into the potential production and/or breeding of decay-resistant hybrid cultivars well suited for such regions.
Prophage Carriage and Genetic Diversity within Environmental Isolates of Clostridioides difficile
Authors: Blau Khald , Gallert Claudia
Published: December 2023

Clostridioides difficile is an important human pathogen causing antibiotic-associated diarrhoea worldwide. Besides using antibiotics for treatment, the interest in bacteriophages as an alternative therapeutic option has increased. Prophage abundance and genetic diversity are well-documented in clinical strains, but the carriage of prophages in environmental strains of C. difficile has not yet been explored. Thus, the prevalence and genetic diversity of integrated prophages in the genomes of 166 environmental C. difficile isolates were identified. In addition, the clustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems were determined in the genomes of prophage regions. Predicted prophages and CRISPR-Cas systems were identified by using the PHASTER web server and CRISPRCasFinder, respectively. Phylogenetic relationships among predicated prophages were also constructed based on phage-related genes, terminase large (TerL) subunits and LysM. Among 372 intact prophages, the predominant prophages were phiCDHM1, phiCDHM19, phiMMP01, phiCD506, phiCD27, phiCD211, phiMMP03, and phiC2, followed by phiMMP02, phiCDKM9, phiCD6356, phiCDKM15, and phiCD505. Two newly discovered siphoviruses, phiSM101- and phivB_CpeS-CP51-like Clostridium phages, were identified in two C. difficile genomes. Most prophages were found in sequence types (STs) ST11, ST3, ST8, ST109, and ST2, followed by ST6, ST17, ST4, ST5, ST44, and ST58. An obvious correlation was found between prophage types and STs/ribotypes. Most predicated prophages carry CRISPR arrays. Some prophages carry several gene products, such as accessory gene regulator (Agr), putative spore protease, and abortive infection (Abi) systems. This study shows that prophage carriage, along with genetic diversity and their CRISPR arrays, may play a role in the biology, lifestyle, and fitness of their host strains.
Biological Effects of “Inflammageing” on Human Oral Cells: Insights into a Potential Confounder of Age-Related Diseases
Authors: Alexakou Elli , Bakopoulou Athina , Apatzidou Danae A. , Kritis Aristeidis , Malousi Andigoni , Anastassiadou Vassiliki
Published: December 2023

Objectives: The term “inflammageing” describes the process of inflammation-induced aging that leads living cells to a state of permanent cell cycle arrest due to chronic antigenic irritation. This in vitro study aimed to shed light on the mechanisms of “inflammageing” on human oral cells. Methods: Primary cultures of human gingival fibroblasts (hGFs) were exposed to variable pro-inflammatory stimuli, including lipopolysaccharide (LPS), Tumor Necrosis Factor-alpha (TNFa), and gingival crevicular fluid (GCF) collected from active periodontal pockets of systemically healthy patients. Inflammageing was studied through two experimental models, employing either late-passage (“aged”) cells (p. 10) that were exposed to the pro-inflammatory stimuli or early-passage (“young”) cells (p. 1) continuously exposed during a period of several passages (up to p. 10) to the above-mentioned stimuli. Cells were evaluated for the expression of beta-galactosidase activity (histochemical staining), senescence-associated genes (qPCR analysis), and biomarkers related to a Senescence-Associated Secretory Phenotype (SASP), through proteome profile analysis and bioinformatics. Results: A significant increase (p < 0.05) in beta-galactosidase-positive cells was observed after exposure to each pro-inflammatory stimulus. The senescence-associated gene expression included upregulation for CCND1 and downregulation for SUSD6, and STAG1, a profile typical for cellular senescence. Overall, pro-inflammatory priming of late-passage cells caused more pronounced effects in terms of senescence than long-term exposure of early-passage cells to these stimuli. Proteomic analysis showed induction of SASP, evidenced by upregulation of several pro-inflammatory proteins (IL-6, IL-10, IL-16, IP-10, MCP-1, MCP-2, M-CSF, MIP-1a, MIP-1b, TNFb, sTNF-RI, sTNF-RII, TIMP-2) implicated in cellular aging and immune responses. The least potent impact on the induction of SASP was provoked by LPS and the most pronounced by GCF. Conclusion: This study demonstrates that long-term exposure of hGFs to various pro-inflammatory signals induced or accelerated cellular senescence with the most pronounced impact noted for the late-passage cells. The outcome of these analyses provides insights into oral chronic inflammation as a potential confounder of age-related diseases.
Targeting Farnesoid X Receptor in Tumor and the Tumor Microenvironment: Implication for Therapy
Authors: Nenkov Miljana , Shi Yihui , Ma Yunxia , Gaßler Nikolaus , Chen Yuan
Published: December 2023

The farnesoid-X receptor (FXR), a member of the nuclear hormone receptor superfamily, can be activated by bile acids (BAs). BAs binding to FXR activates BA signaling which is important for maintaining BA homeostasis. FXR is differentially expressed in human organs and exists in immune cells. The dysregulation of FXR is associated with a wide range of diseases including metabolic disorders, inflammatory diseases, immune disorders, and malignant neoplasm. Recent studies have demonstrated that FXR influences tumor cell progression and development through regulating oncogenic and tumor-suppressive pathways, and, moreover, it affects the tumor microenvironment (TME) by modulating TME components. These characteristics provide a new perspective on the FXR-targeted therapeutic strategy in cancer. In this review, we have summarized the recent research data on the functions of FXR in solid tumors and its influence on the TME, and discussed the mechanisms underlying the distinct function of FXR in various types of tumors. Additionally, the impacts on the TME by other BA receptors such as takeda G protein-coupled receptor 5 (TGR5), sphingosine-1-phosphate receptor 2 (S1PR2), and muscarinic receptors (CHRM2 and CHRM3), have been depicted. Finally, the effects of FXR agonists/antagonists in a combination therapy with PD1/PD-L1 immune checkpoint inhibitors and other anti-cancer drugs have been addressed.
A Functional Single Nucleotide Polymorphism in the 3′ Untranslated Region of the Porcine JARID2 Gene Is Associated with Aggressive Behavior of Weaned Pigs after Mixing
Authors: Yang Huan , Zhang Chunlei , Chao Xiaohuan , Zhao Jing , Liu Mingzheng , Chen Jiahao , Liu Shuhan , Wang Tianshuo , Muhammad Asim , Schinckel Allan P. , Zhou Bo
Published: December 2023

In pig production, pigs often show more aggressive behavior after mixing, which adversely affects animal welfare and growth performance. The Jumonji and structural domain-rich AT interaction domain 2 (JARID2) gene plays an important role in neurodevelopment in mice and various psychiatric disorders in humans. The JARID2 gene may impact the aggressive behavior of pigs. By observing the behavior of 500 weaned pigs during the first 72 h after mixing, the ear tissue samples of the 12 most aggressive and 12 least aggressive pigs were selected for DNA resequencing based on the intensity of their aggressive behavior. Large group correlation analysis indicated that the rs3262221458 site located in the 3′-UTR region of the porcine JARID2 gene has a strong relationship with the aggressive behavior of weaned pigs. Pigs with the mutant TT genotype of rs3262221458 have more aggressive behavior than those pigs with the GG and GT genotypes. The dual luciferase assay indicated that the luciferase activity of the plasmids containing the G allele of rs326221458 was significantly less than that of plasmids containing the T allele of rs326221458 and control groups. The binding ability of miR-9828-3p to sequences containing the T allele was less than that of sequences containing the G allele. The overexpression of miR-9828-3p in porcine neuroglial cells (PNGCs) and PK15 cells significantly decreased the mRNA and protein levels of the JARID2 gene. In addition, miR-9828-3p inhibited the proliferation of PNGCs. After inhibiting miR-9828-3p, the mRNA and protein expression levels of JARID2 increased, and the proliferation of PNGCs showed an opposite trend to the cells that forced the expression of miR-9828-3p. In addition, interference with the JARID2 gene by siRNA can effectively inhibit the proliferation of PNGCs. In summary, we found that the rs326221458 locus regulates the expression of the JARID2 gene by affecting the binding of miR-9828-3p and the JARID2 gene, thereby affecting the aggressive behavior of weaned pigs after mixing.
Chronic Stress Blocks the Endometriosis Immune Response by Metabolic Reprogramming
Authors: Lu Chong , Xu Jing , Li Ke , Wang Jing , Dai Yilin , Chen Yiqing , Chai Ranran , Xu Congjian , Kang Yu
Published: December 2023

Studies have shown that the occurrence and development of endometriosis are closely linked to long-term psychological stress. The specific contribution of chronic stress to the metabolic adaptations in patients with endometriosis is still unknown. Lesions were removed from ten endometriosis patients during an operation, and the participants were divided into two groups using a psychological questionnaire. An mRNA Human Gene Expression Microarray analysis was applied to compare the mRNA expression profiles between the chronic stress group and the control group. In addition, the reliability of the mRNA Human Gene Expression Microarray analysis was verified by using research on metabolites based on both the liquid chromatography (LC-MS/MS) technique and quantitative reverse transcription polymerase chain reaction (RT–PCR). A microarray analysis of significantly up-regulated, differentially expressed genes between the chronic stress and the control groups showed genes that were principally related to metabolism-related processes and immune-related processes, such as the immune response process, negative regulation of T cell proliferation, the leucine metabolic process, and the L-cysteine metabolic process (p < 0.05). LC-MS showed that the differential metabolites were primarily concerned with arginine and proline metabolism, D-glutamine and D-glutamate metabolism, aspartate metabolism, glycine, serine metabolism, and tyrosine metabolism (p < 0.05). The possibility of chronic stress blocks the endometriosis immune response through metabolic reprogramming. Chronic stress reduces the supply of energy substrates such as arginine and serine, down-regulates T immune cell activation, and affects the anti-tumor immune response, thereby promoting the migration and invasion of endometriosis lesions in patients with chronic stress.
Serum-Resistant Ternary DNA Polyplexes for Suicide Gene Therapy of Uterine Leiomyoma
Authors: Egorova Anna , Shtykalova Sofia , Maretina Marianna , Freund Svetlana , Selutin Alexander , Shved Natalia , Selkov Sergei , Kiselev Anton
Published: December 2023

Uterine leiomyoma (UL) is a prevalent benign tumor in women that frequently gives rise to a multitude of reproductive complications. The use of suicide gene therapy has been proposed as a highly promising method for treating UL. To achieve successful gene therapy, it is essential to develop carriers that can efficiently transport nucleic acids into targeted cells and tissues. The instability of polyplexes in blood and other biological fluids is a crucial factor to consider when using non-viral carriers. In this study, we present serum-resistant and cRGD-modified DNA complexes for targeted delivery genes to UL cells. Ternary polyplexes were formed by incorporating cystine-cross-linked polyglutamic acid modified with histidine residues. We employed two techniques in the production of cross-linked polyanionic coating: matrix polymerization and oxidative polycondensation. In this study, we investigated the physicochemical properties of ternary DNA complexes, including the size and zeta-potential of the nanoparticles. Additionally, we evaluated cellular uptake, toxicity levels, transfection efficiency and specificity in vitro. The study involved introducing the HSV-TK gene into primary UL cells as a form of suicide gene therapy modeling. We have effectively employed ternary peptide-based complexes for gene delivery into the UL organtypic model. By implementing in situ suicide gene therapy, the increase in apoptosis genes expression was detected, providing conclusive evidence of apoptosis occurring in the transfected UL tissues. The results of the study strongly suggest that the developed ternary polyplexes show potential as a valuable tool in the implementation of suicide gene therapy for UL.
Mutanolysin-Digested Peptidoglycan of Lactobacillus reuteri Promotes the Inhibition of Porphyromonas gingivalis Lipopolysaccharide-Induced Inflammatory Responses through the Regulation of Signaling Cascades via TLR4 Suppression
Authors: Kim Donghan , Choi Hanhee , Oh Hyeonjun , Lee Jiyeon , Hwang Yongjin , Kang Seok-Seong
Published: December 2023

Periodontitis is an oral infectious disease caused by various pathogenic bacteria, such as Porphyromonas gingivalis. Although probiotics and their cellular components have demonstrated positive effects on periodontitis, the beneficial impact of peptidoglycan (PGN) from probiotic Lactobacillus remains unclear. Therefore, our study sought to investigate the inhibitory effect of PGN isolated from L. reuteri (LrPGN) on P. gingivalis-induced inflammatory responses. Pretreatment with LrPGN significantly inhibited the production of interleukin (IL)-1β, IL-6, and CCL20 in RAW 264.7 cells induced by P. gingivalis lipopolysaccharide (LPS). LrPGN reduced the phosphorylation of PI3K/Akt and MAPKs, as well as NF-κB activation, which were induced by P. gingivalis LPS. Furthermore, LrPGN dose-dependently reduced the expression of Toll-like receptor 4 (TLR4), indicating that LrPGN inhibits periodontal inflammation by regulating cellular signaling cascades through TLR4 suppression. Notably, LrPGN exhibited stronger inhibition of P. gingivalis LPS-induced production of inflammatory mediators compared to insoluble LrPGN and proteinase K-treated LrPGN. Moreover, MDP, a minimal bioactive PGN motif, also dose-dependently inhibited P. gingivalis LPS-induced inflammatory mediators, suggesting that MDP-like molecules present in the LrPGN structure may play a crucial role in the inhibition of inflammatory responses. Collectively, these findings suggest that LrPGN can mitigate periodontal inflammation and could be a useful agent for the prevention and treatment of periodontitis.
Synthesis and Characterization of Poly(Butylene Sebacate-Co-Terephthalate) Copolyesters with Pentaerythritol as Branching Agent
Authors: Jang Hyunho , Kwon Sangwoo , Kim Sun Jong , Kim Young-Teck , Park Su-il
Published: December 2023

Poly(butylene sebacate-co-terephthalate) (PBSeT) copolyesters are prepared by melt polymerization via two-step transesterification and polycondensation using pentaerythritol (PE) as a branching agent. The effects of the incorporated PE on its chemical, thermal, mechanical, and degradation properties, along with the rheological properties of its melt, are investigated. The highest molecular weight and intrinsic viscosity along with the lowest melt flow index were achieved at a PE content of 0.2 mol%, with minimal reduction in the tensile strength and the highest tear strength. The addition of PE did not significantly influence the thermal behavior and stability of the PBSeT copolyesters; however, the elongation at break decreased with increasing PE content. The sample with 0.2 mol% PE exhibited a higher storage modulus and loss modulus as well as a lower loss angle tangent than the other samples, indicating improved melt elasticity. The incorporation of more than 0.2 mol% PE enhanced the enzymatic degradation of copolyesters. In summary, including within 0.2 mol%, PE effectively improved both the processability-related characteristics and degradation properties of PBSeT copolyesters, suggesting their potential suitability for use in agricultural and packaging materials.
Engineering Band Gap of Ternary Ag2TexS1−x Quantum Dots for Solution-Processed Near-Infrared Photodetectors
Authors: Wang Zan , Gu Yunjiao , Aleksandrov Daniil , Liu Fenghua , He Hongbo , Wu Weiping
Published: December 2023

Silver-based chalcogenide semiconductors exhibit low toxicity and near-infrared optical properties and are therefore extensively employed in the field of solar cells, photodetectors, and biological probes. Here, we report a facile mixture precursor hot-injection colloidal route to prepare Ag2TexS1−x ternary quantum dots (QDs) with tunable photoluminescence (PL) emissions from 950 nm to 1600 nm via alloying band gap engineering. As a proof-of-concept application, the Ag2TexS1−x QDs-based near-infrared photodetector (PD) was fabricated via solution-processes to explore their photoelectric properties. The ICP-OES results reveal the relationship between the compositions of the precursor and the samples, which is consistent with Vegard’s equation. Alloying broadened the absorption spectrum and narrowed the band gap of the Ag2S QDs. The UPS results demonstrate the energy band alignment of the Ag2Te0.53S0.47 QDs. The solution-processed Ag2TexS1−x QD-based PD exhibited a photoresponse to 1350 nm illumination. With an applied voltage of 0.5 V, the specific detectivity is 0.91 × 1010 Jones and the responsivity is 0.48 mA/W. The PD maintained a stable response under multiple optical switching cycles, with a rise time of 2.11 s and a fall time of 1.04 s, which indicate excellent optoelectronic performance.
Flywheel Romanian Deadlift: Intra- and Inter-Day Kinetic and Kinematic Reliability of Four Inertial Loads Using Cluster Sets
Authors: Ryan Shane , Ramirez-Campillo Rodrigo , Browne Declan , Moody Jeremy , Byrne Paul J.
Published: December 2023

The primary aim of this study was to investigate the intra- and inter-day reliability of flywheel cluster set training in concentric power (CON), eccentric power (ECC), and ECC overload during the Romanian deadlift exercise (RDL). A secondary aim was to assess the acute effect of internal and external attentional focus instructions on mean power when performing the flywheel RDL. Fourteen collegiate male field sport athletes (age, 23.3 ± 3.7 years; mass, 80.8 ± 9.9 kg; height, 1.79 ± 0.06 m) were randomized into internal (n = 7) or external (n = 7) attentional focus groups and attended four testing sessions, with a between-session separation of 7 days. Sessions consisted of four cluster sets of fifteen repetitions “excluding momentum repetitions” (4 × (5 + 5 + 5)) using a specific inertial load (0.025, 0.050, 0.075, and 0.100 kg·m−2) for a given set in a randomized ascending or descending order. Cluster sets were separated by a 45 s intra-set rest period. Both instructional focus groups attained familiarization, although the time taken to achieve familiarization (outcome stability) differed between groups. The external instructional group attained familiarization post-session 2 (Cohen’s d (ES), ES = 0.11–0.65) with little volatility between performance measures (CV% = 4.61–9.59). Additionally, the internal group reported inconsistencies among all inertial loads, reporting large differences in MP in the 0.100 kg·m−2 inertial load from day 2 to day 3 (ES = 1.22) and both 0.050 kg·m2 (p = 0.010) and 0.075 kg·m−2 (p = 0.016) between day 3 and day 4. The flywheel RDL cluster set approach is a reliable training modality for maintaining mean power output during cluster set repetitions.
Analysis of Stress–Strain Characteristics and Signal Coherence of Low-Specific-Speed Impeller Based on Fluid–Structure Interaction
Authors: Qiao Fengquan , Sun Yi , Zhu Di , Fang Mingkun , Zhang Fangfang , Tao Ran , Xiao Ruofu
Published: December 2023

In this study, an analysis of a low-specific-speed pump is carried out based on the methods of one-way and two-way fluid–structure interactions (FSIs). This study analyzes the influence of FSIs on the internal flow field and external characteristics of the pump. Utilizing a two-way FSI, the signal coherence analysis method is employed to analyze the coherence of signals between the flow field and the structural field. Addressing the issue of a lack of a connection between the two signals, this study bridges a gap in the existing research. The results indicate that different interaction methods have certain influences on impeller stress and deformation. However, in both coupling modes, the maximum deformation and the maximum equivalent stress have the same distribution position. The head error obtained using the two-way coupling method is lower than that of the uncoupled results, which indicates that the two-way FSI calculation results are closer to the experimental results. The pressure pulsation signals at the interface of the impeller and volute exhibit strong coherence with the structural field signals. For low-specific-speed centrifugal pumps, establishing a clear connection between the flow field signals and structural field signals will help guide further optimization of their performance through design.
Leg Mechanism Design and Motion Performance Analysis for an Amphibious Crab-like Robot
Authors: Hu Shihao , Ma Xinmeng , Chen Xi , Xin Mingfei , Tian Changda , Liu Kaixin , Li Sicen , Wang Liquan , Tang Qinyun , Liu Zhaojin , Ding Mingxuan , Li Jiawei
Published: December 2023

Bionic-legged robots draw inspiration from animal locomotion methods and structures, demonstrating the potential to traverse irregular and unstructured environments. The ability of Portunus trituberculatus (Portunus) to run flexibly and quickly in amphibious environments inspires the design of systems and locomotion methods for amphibious robots. This research describes an amphibious crab-like robot based on Portunus and designs a parallel leg mechanism for the robot based on biological observations. The research creates the group and sequential gait commonly used in multiped robots combined with the form of the robot’s leg mechanism arrangement. This research designed the parallel leg mechanism and modeled its dynamics. Utilizing the outcomes of the dynamics modeling, we calculate the force and torque exerted on each joint of the leg mechanism during group gait and sequential gait when the robot is moving with a load. This analysis aims to assess the performance of the robot’s motion. Finally, a series of performance evaluation experiments are conducted on land and underwater, which show that the amphibious crab-like robot has good walking performance. The crab-like robot can perform forward, backward, left, and right walking well using group and sequential gaits. Simultaneously, the crab-like robot showcases faster movement in group gaits and a more substantial load capacity in sequential gaits.
Differential Influences of High-Speed Railway Stations on the Surrounding Construction Land Expansion and Institutional Analysis: The Case of Taiwan and Hainan
Authors: Zhou Bo , Hu Xiaofei , Xiong Changsheng
Published: December 2023

The purpose of this paper is to compare the differential impact of Taiwan High-speed Railway (T-HSR) and Hainan East Ring High-speed Railway (HER-HSR) stations on the surrounding construction land expansion, reveal the underlying institutional reasons, and then provide policy recommendations for coordinating the rational development of land resources and ecological environment protection around high-speed railway (HSR) stations. The research methods include the Institutional Analysis and Development (IAD) framework, buffer analysis, and GIS spatial analysis. The results show that (1) The spillover impact of HSR stations on the surrounding construction land expansion is the result of the game between different actors around the development and protection of land around HSR stations under the action scenarios of various external factors. The surrounding construction land of 12 stations of T-HSR increased by 269.98 km2 within the study scope and period, while the surrounding area of 10 stations in HER-HSR increased by 187.61 km2. (2) There are nine stations in T-HSR that affect the expansion of surrounding construction land within 1.0–3.0 km and lead to the expansion of construction land of 22.44 km2, while there are six stations in HER-HSR that affect the expansion of construction land within 1.5–3.5 km and lead to the expansion of construction land of 24.20 km2. Other stations cannot effectively identify their areas of influence due to other reasons. (3) Each station of HER-HSR has a stronger influence intensity and degree on the expansion of surrounding construction land than that of T-HSR, which is closely related to institutional factors such as land planning, land property rights, and public participation. In conclusion, the differences in land planning, land property rights, public participation, and other basic systems will lead to differences in the expansion of surrounding construction land for HSR stations. This research aims to provide institutional adjustment suggestions to achieve coordinated economic development and ecological protection around HSR stations in two Chinese islands with different institutional environments.
Karenia brevis Extract Induces Cellular Entry through Distinct Mechanisms in Phagocytic RAW 264.7 Macrophages versus Non-Phagocytic Vero Cells
Authors: Minns Laurie , Sausman Kathryn , Brown Ariel , York Robert , McCall Jennifer
Published: December 2023

Marine algae extracts are an important area of potential drug discovery; however, nearly all studies to date have used non-fluorescent-based methods to determine changes in target cell activity. Many of the most robust immunological and cellular analyses rely on fluorescent probes and readouts, which can be problematic when the algae extract is fluorescent itself. In this study, we identified the fluorescent spectrum of an isolated extract from the marine dinoflagellate Karenia brevis, which included two fluorescing components: chlorophyll α and pheophytin α. When excited at 405 nm and 664 nm, the extract emitted fluorescence at 676 nm and 696 nm, respectively. The extract and its fluorescing components, chlorophyll α and pheophytin α, entered phagocytic RAW 264.7 macrophages and non-phagocytic Vero kidney cells through distinct mechanisms. When incubated with the extract and its main components, both the RAW 264.7 macrophages and the Vero cells accumulated fluorescence as early as 30 min and continued through 48 h. Vero kidney cells accumulated the K. brevis fluorescent extract through a dynamin-independent and acidified endosomal-dependent mechanism. RAW 264.7 macrophages accumulated fluorescent extract through a dynamin-independent, acidified endosomal-independent mechanism, which supports accumulation through phagocytosis. Furthermore, RAW 264.7 macrophages downregulated cell-surface expression of CD206 in response to extract stimulation indicating activation of phagocytic responses and potential immunosuppression of these immune cells. This study represents the first characterization of the cellular update of K. brevis extracts in phagocytic versus non-phagocytic cells. The data suggest the importance of understanding cellular uptake of fluorescing algae extracts and their mechanism of action for future drug discovery efforts.
Chewing Gum May Alleviate Degree of Thirst in Patients on Hemodialysis
Authors: Chen Yi-Qi , Wang Chia-Liang , Chiu Ai-Hua , Yeh Ming-Chen , Chiang Tsay-I
Published: December 2023

Background and Objectives: Fluid restriction commonly causes dry mouth in patients on hemodialysis (HD). The observed effects of chewing gum on the amount of saliva, interdialytic weight gain (IDWG), and degree of thirst in patients on HD have been inconsistent. We investigated whether chewing gum can modulate these three parameters in patients on HD. Materials and Methods: We used purposive sampling to enroll 37 patients on HD who were then randomly divided into the chewing gum group (n = 19) and the control (non–chewing gum) group (n = 18). The degree of thirst was assessed using a self-rated visual analog scale (VAS) and a summated xerostomia inventory (SXI), respectively. Results: The degree of thirst after dialysis was significantly lower in the chewing gum group than in the control group. The amount of saliva was higher in the chewing gum group than in the control group before dialysis, but the saliva amount was comparable between the groups after dialysis. Furthermore, the amount of saliva was not associated with IDWG in patients on HD before or after dialysis. Conclusions: Chewing gum may alleviate the feeling of thirst but may not affect the amount of saliva and IDWG in patients on HD. Therefore, we suggest that chewing gum may be a useful approach to improve dry mouth and thirst in patients on HD.
Assessing Genomic Mutations in SARS-CoV-2: Potential Resistance to Antiviral Drugs in Viral Populations from Untreated COVID-19 Patients
Authors: Lombardo Daniele , Musolino Cristina , Chines Valeria , Caminiti Giuseppe , Palermo Claudia , Cacciola Irene , Raffa Giuseppina , Pollicino Teresa
Published: December 2023

Naturally occurring SARS-CoV-2 variants mutated in genomic regions targeted by antiviral drugs have not been extensively studied. This study investigated the potential of the RNA-dependent RNA polymerase (RdRp) complex subunits and non-structural protein (Nsp)5 of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) to accumulate natural mutations that could affect the efficacy of antiviral drugs. To this aim, SARS-CoV-2 genomic sequences isolated from 4155 drug-naive individuals from southern Italy were analyzed using the Illumina MiSeq platform. Sequencing of the 4155 samples showed the following viral variant distribution: 71.2% Delta, 22.2% Omicron, and 6.4% Alpha. In the Nsp12 sequences, we found 84 amino acid substitutions. The most common one was P323L, detected in 3777/4155 (91%) samples, with 2906/3777 (69.9%) also showing the G671S substitution in combination. Additionally, we identified 28, 14, and 24 different amino acid substitutions in the Nsp5, Nsp7, and Nsp8 genomic regions, respectively. Of note, the V186F and A191V substitutions, affecting residues adjacent to the active site of Nsp5 (the target of the antiviral drug Paxlovid), were found in 157/4155 (3.8%) and 3/4155 (0.07%) samples, respectively. In conclusion, the RdRp complex subunits and the Nsp5 genomic region exhibit susceptibility to accumulating natural mutations. This susceptibility poses a potential risk to the efficacy of antiviral drugs, as these mutations may compromise the drug ability to inhibit viral replication
Independent Risk Factors Predicting Eradication Failure of Hybrid Therapy for the First-Line Treatment of Helicobacter pylori Infection
Authors: Chen Chien-Lin , Wu I-Ting , Wu Deng-Chyang , Lei Wei-Yi , Tsay Feng-Woei , Chuah Seng-Kee , Chen Kuan-Yang , Yang Jyh-Chin , Liu Yu-Hwa , Kuo Chao-Hung , Shiu Sz-Iuan , Shie Chang-Bih , Lin Kuan-Hua , Lee Chia-Long , Hsu Ping-I
Published: December 2023

Hybrid therapy is a recommended first-line anti-H. pylori treatment option in the American College of Gastroenterology guidelines, the Bangkok Consensus Report on H. pylori management, and the Taiwan H. pylori Consensus Report. However, the cure rates of eradication therapy in some countries are suboptimal, and the factors affecting the treatment efficacy of hybrid therapy remain unclear. The aim of this study is to identify the independent risk factors predicting eradication failure of hybrid therapy in the first-line treatment of H. pylori infection. A retrospective cohort study was conducted on 589 H. pylori-infected patients who received 14-day hybrid therapy between September 2008 and December 2021 in ten hospitals in Taiwan. The patients received a hybrid therapy containing a dual regimen with a proton pump inhibitor (PPI) plus amoxicillin for an initial 7 days and a quadruple regimen with a PPI plus amoxicillin, metronidazole and clarithromycin for a final 7 days. Post-treatment H. pylori status was assessed at least 4 weeks after completion of treatment. The relationships between eradication rate and 13 host and bacterial factors were investigated via univariate and multivariate analyses. In total, 589 patients infected with H. pylori infection were included in the study. The eradication rates of hybrid therapy were determined as 93.0% (95% confidence interval (CI): 90.9–95.1%), 94.4% (95% CI: 93.8–97.2%) and 95.5%% (95% CI: 93.8–97.2%) by intention-to-treat, modified intention-to-treat and per-protocol analyses, respectively. Univariate analysis showed that the eradication rate of clarithromycin-resistant strains was lower than that of clarithromcyin-susceptible strains (83.3% (45/54) vs. 97.6%% (280/287); p < 0.001). Subjects with poor drug adherence had a lower cure rate than those with good adherence (73.3% (11/15) vs. 95.5% (534/559); p = 0.005). Other factors such as smoking, alcohol drinking, coffee consumption, tea consumption and type of PPI were not significantly associated with cure rate. Multivariate analysis revealed that clarithromcyin resistance of H. pylori and poor drug adherence were independent risk factors related to eradication failure of hybrid therapy with odds ratios of 4.8 (95% CI: 1.5 to 16.1; p = 0.009) and 8.2 (95% CI: 1.5 to 43.5; p = 0.013), respectively. A 14-day hybrid therapy has a high eradication rate for H. pylori infection in Taiwan, while clarithromycin resistance of H. pylori and poor drug adherence are independent risk factors predicting eradication failure of hybrid therapy.
Extreme Gradient Boosting Combined with Conformal Predictors for Informative Solubility Estimation
Authors: Jovic Ozren , Mouras Rabah
Published: December 2023

We used the extreme gradient boosting (XGB) algorithm to predict the experimental solubility of chemical compounds in water and organic solvents and to select significant molecular descriptors. The accuracy of prediction of our forward stepwise top-importance XGB (FSTI-XGB) on curated solubility data sets in terms of RMSE was found to be 0.59–0.76 Log(S) for two water data sets, while for organic solvent data sets it was 0.69–0.79 Log(S) for the Methanol data set, 0.65–0.79 for the Ethanol data set, and 0.62–0.70 Log(S) for the Acetone data set. That was the first step. In the second step, we used uncurated and curated AquaSolDB data sets for applicability domain (AD) tests of Drugbank, PubChem, and COCONUT databases and determined that more than 95% of studied ca. 500,000 compounds were within the AD. In the third step, we applied conformal prediction to obtain narrow prediction intervals and we successfully validated them using test sets’ true solubility values. With prediction intervals obtained in the last fourth step, we were able to estimate individual error margins and the accuracy class of the solubility prediction for molecules within the AD of three public databases. All that was possible without the knowledge of experimental database solubilities. We find these four steps novel because usually, solubility-related works only study the first step or the first two steps.
Research on the Structure Design of Silicon Avalanche Photodiode with Near-Ultraviolet High Responsivity
Authors: Guo Guangtong , Chen Weishuai , Zheng Kaifeng , Lv Jinguang , Qin Yuxin , Zhao Baixuan , Zhao Yingze , Chen Yupeng , Gao Dan , Liang Jingqiu , Wang Weibiao
Published: December 2023

To improve the low responsivity of the silicon avalanche photodiode in the near-ultraviolet wavelength range, we designed a near-ultraviolet highly responsive Si-APD basic structure with a multiplication layer neighboring the photosensitive surface through the analysis of the optical absorption characteristics, junction breakdown characteristics, and avalanche multiplication characteristics. The dark current and electric field distribution of the device were investigated. Meanwhile, the structural parameters of the surface non-depleted layer, multiplication layer, and absorption layer were optimized. It was found that the breakdown voltage of the device is 21.07 V. At an applied bias voltage of 20.02 V, the device exhibits a responsivity of 6.79–14.51 A/W in the wavelength range of 300–400 nm. These results provide valuable insights for the design of silicon avalanche photodiode with high responsivity in the near-ultraviolet range.