bluuebunny/crossref_metadata_2025 huggingface dataset:
Fermented Foods as a Potential Vehicle of Antimicrobial-Resistant Bacteria and Genes
Authors: Vinayamohan Poonam Gopika , Viju Leya Susan , Joseph Divya , Venkitanarayanan Kumar
Published: July 2023

Fermented food products are widely consumed for their nutritional and health-promoting properties, earning them a central place in diets around the globe. However, these foods can present a paradox, as they have the potential to harbor not only beneficial probiotics but also antibiotic-resistant (AR) microbes and genes. The impact of AR microbes and genes in fermented foods has far-reaching implications, such as potential effects on human health, repercussions in the food industry, and environmental consequences. An in-depth analysis of AR microbes and genes in fermented foods, including dairy products, fermented fruits and vegetables, meat products, and beverages, would provide insights into the extent and ramifications of the issue with these foods. Therefore, this review systematically presents the status of AR in fermented foods, with a particular focus on AR bacteria and genes within this category of food products. The review also highlights the complexities of AR in fermented foods, emphasizing the role of bacterial adaptation during the fermentation process and the dynamics of bacterial gene transfer. Various factors contributing to AR microbes and genes are brought into focus, including intrinsic resistance among bacteria in fermented foods and the potential risk of contamination with pathogenic bacteria. Moreover, this review presents a range of mitigation strategies, from the development of novel antimicrobials to advances in fermentation technology and regulatory control. This comprehensive perspective on the intricate interplay between AR and fermented food will potentially pave the way for more targeted research and mitigation strategies in this critical area.
Unveiling the Landscape of Operating System Vulnerabilities
Authors: Bhurtel Manish , Rawat Danda B.
Published: July 2023

Operating systems play a crucial role in computer systems, serving as the fundamental infrastructure that supports a wide range of applications and services. However, they are also prime targets for malicious actors seeking to exploit vulnerabilities and compromise system security. This is a crucial area that requires active research; however, OS vulnerabilities have not been actively studied in recent years. Therefore, we conduct a comprehensive analysis of OS vulnerabilities, aiming to enhance the understanding of their trends, severity, and common weaknesses. Our research methodology encompasses data preparation, sampling of vulnerable OS categories and versions, and an in-depth analysis of trends, severity levels, and types of OS vulnerabilities. We scrape the high-level data from reliable and recognized sources to generate two refined OS vulnerability datasets: one for OS categories and another for OS versions. Our study reveals the susceptibility of popular operating systems such as Windows, Windows Server, Debian Linux, and Mac OS. Specifically, Windows 10, Windows 11, Android (v11.0, v12.0, v13.0), Windows Server 2012, Debian Linux (v10.0, v11.0), Fedora 37, and HarmonyOS 2, are identified as the most vulnerable OS versions in recent years (2021–2022). Notably, these vulnerabilities exhibit a high severity, with maximum CVSS scores falling into the 7–8 and 9–10 range. Common vulnerability types, including CWE-119, CWE-20, CWE-200, and CWE-787, are prevalent in these OSs and require specific attention from OS vendors. The findings on trends, severity, and types of OS vulnerabilities from this research will serve as a valuable resource for vendors, security professionals, and end-users, empowering them to enhance OS security measures, prioritize vulnerability management efforts, and make informed decisions to mitigate risks associated with these vulnerabilities.
Socio-Psychological, Economic and Environmental Effects of Forest Fires
Authors: Kalogiannidis Stavros , Chatzitheodoridis Fotios , Kalfas Dimitrios , Patitsa Christina , Papagrigoriou Aristidis
Published: July 2023

One of the most common forest disturbances, fire, has a significant influence on the people, societies, economies, and environment of countries all over the world. This study explores the different environmental and socioeconomic effects of forest fires to establish priorities for countries in battling and mitigating the harmful effects of forest fires based on data collected from 382 professionals working in Greece’s forestry and agriculture sectors. Secondary data, especially from Statista, were further utilized to enhance the analytical comparisons and conclusions of this study. Wildfires in Greece destroy agricultural land and greatly impact the rural economy and community. This study showed that forest fires have led to several economic costs, mainly affecting the incomes of different investors in the forest sector in Greece. It was revealed that the overall cost of a fire is determined by the direct and indirect expenditures as well as the price of fire control and preventative methods. Direct expenses are broken down into two categories: direct damage that occurs immediately and direct losses that are caused immediately after a fire. Governments should take the initiative to create and expand bilateral and/or multilateral cooperation and coordination, as well as exchange necessary financial resources, technology, and training, to reduce the effects of forest fires in a fragile international man-made and natural environment.
Biochemical Patterns and Genotoxicity of the Endocrine Disruptor Metformin in the Freshwater Fish Labeo rohita
Authors: Sibiya Ashokkumar , Al-Ghanim Khalid A. , Govindarajan Marimuthu , Nicoletti Marcello , Sachivkina Nadezhda , Vaseeharan Baskaralingam
Published: July 2023

Metformin is one of the most extensively used drugs, making it one of the most likely endocrine disruptors in the environment, which may negatively affect fish and other freshwater animals. Still, there is a dearth of studies examining metformin’s impact on freshwater creatures, like fish. This research aimed to identify the biochemical and genotoxicity effects of the endocrine disruptor metformin in the freshwater fish Labeo rohita at ecologically appropriate doses. Metformin’s toxicity was evaluated by subjecting L. rohita to the drug over 28 days at two dosages (40 µg/L to 80 µg/L). The results indicated that 40 µg/L and 80 µg/L of metformin caused an increase in reactive oxygen species and the generation of free radicals in the body of L. rohita, which in turn caused impairment and alterations in total hemoglobin, red blood corpuscles, white blood corpuscles, oxidative stress, lipid peroxidation, protein carbonyl activity, respiratory burst activity, myeloperoxidase activity, and lysozyme activity. In addition, animals treated with the maximum metformin dose (80 µg/L) demonstrated substantial DNA damage in the genotoxicity experiment. Metformin’s endocrine-disrupting actions may have unintended ramifications for the well-being of aquatic species in their natural habitats. Results of the study demonstrated a serious concern that metformin exposure might be harmful to aquatic life.
Amino Acid Variation at the Mitochondrial Binding Site of Antimycin A is Proposed to Reflect Sensitivity and Toxicity Differences among Fish Species
Authors: Baudoin Brooke A. , Brown Bonnie L. , Calfee Robin D. , Jenkins Jill A.
Published: July 2023

To better understand differential sensitivities among fish species to the piscicidal compound Antimycin-A (ANT-A), we hypothesized that variations in amino acids at the ANT-A binding site may reflect toxicity differences. Protein sequences for six motifs comprising the ANT-A binding site were obtained and compared for invasive carp species (N = 515) and seven non-target species (N = 277); a consensus was delineated from each species. The carp species, Common Carp (Cyprinus carpio), Silver Carp (Hypophthalmichthys molitrix), Bighead Carp (Hypophthalmichthys nobilis), Grass Carp (Ctenopharyngodon idella), and Black Carp (Mylopharyngodon piceus), showed the same amino acids at the site; thus, it was termed the carp consensus motif sequence (CCM). Channel Catfish (Ictalurus punctatus) showed the most amino acid polymorphisms, with three motifs 96–100% different from CCM. Within a species, Bluegill (Lepomis macrochirus) and Fathead Minnow (Pimephales promelas) variation per motif was most dissimilar (46.7% and 21.6%, respectively). Organismal mortality data from the literature indicated Yellow Perch (Perca flavescens), Walleye (Sander vitreus), and American Gizzard Shad (Dorosoma cepedianum) to be most sensitive to the piscicide, Catfish least sensitive, and all others intermediate. The protein sequence variations of the binding site appeared to be in accord with organismal sensitivity categories when they differed from the CCM; the motifs in Gizzard Shad and Walleye were the same as in CCM. The physical/chemical nature of ANT-A is important to consider in organismal response comparisons. This cellular approach of studying ANT-A binding at its target enzyme is a non-destructive way to predict piscicidal efficacy of ANT-A against fishes of interest, informs management decisions in control efforts for invasives, and can be used to forecast effects on sympatric species.
Bulk Cavitation in Model Gasoline Injectors and Their Correlation with the Instantaneous Liquid Flow Field
Authors: Kolokotronis Dimitrios , Sahu Srikrishna , Hardalupas Yannis , Taylor Alex M. K. P. , Arioka Akira
Published: July 2023

It is well established that spray characteristics from automotive injectors depend on, among other factors, whether cavitation arises in the injector nozzle. Bulk cavitation, which refers to the cavitation development distant from walls and thus far from the streamline curvature associated with salient points on a wall, has not been thoroughly investigated experimentally in injector nozzles. Consequently, it is not clear what is causing this phenomenon. The research objective of this study was to visualize cavitation in three different injector models (designated as Type A, Type B, and Type C) and quantify the liquid flow field in relation to the bulk cavitation phenomenon. In all models, bulk cavitation was present. We expected this bulk cavitation to be associated with a swirling flow with its axis parallel to that of the nozzle. However, liquid velocity measurements obtained through particle image velocimetry (PIV) demonstrated the absence of a swirling flow structure in the mean flow field just upstream of the nozzle exit, at a plane normal to the hypothetical axis of the injector. Consequently, we applied proper orthogonal decomposition (POD) to analyze the instantaneous liquid velocity data records in order to capture the dominant coherent structures potentially related to cavitation. It was found that the most energetic mode of the liquid flow field corresponded to the expected instantaneous swirling flow structure when bulk cavitation was present in the flow.
Hazelnut Skin Waste as a Functional Ingredient to Nutritionally Improve a Classic Shortbread Cookie Recipe
Authors: Costantini Lara , Frangipane Maria Teresa , Molinari Romina , Garzoli Stefania , Massantini Riccardo , Merendino Nicolò
Published: July 2023

Hazelnut skin (HS) is a byproduct of hazelnut processing (2.5% of total kernel) and becomes a food waste despite its high content of antioxidants, unsaturated fats, and fibers. Classic shortbread cookies have a large worldwide market, even if their nutritional composition does not meet nutritional guidelines due to the high content of saturated fats. In the present study, after the nutritional evaluation of four different HS varieties, 5% and 10% ratios of HS of the Tonda Gentile Romana variety were integrated into a classic shortbread cookie recipe, proportionally replacing the butter amount with the unsaturated fats naturally present in HS. The 10% HS addition determined a 20% increase in the monounsaturated oleic acid and a 15.7% decrease in the saturated palmitic acid, in addition to a significant ash increase. The sensory analysis revealed higher consumer acceptance of the 5% formulation, with scores comparable to the control. Although the 10% formulation obtained lower scores for consumer acceptance, 35% of the interviewed population said they would purchase it, indicating that this product, beyond the ethical dimension of using a food waste matrix to promote the circular economy, can attract the commercial interest of part of the population.
Black Queens of Fruits: Chemical Composition of Blackberry (Rubus subg. rubus Watson) and Black Currant (Ribes nigrum L.) Cultivars Selected in Serbia
Authors: Karaklajic-Stajic Zaklina , Tomic Jelena , Pesakovic Marijana , Paunovic Svetlana M. , Stampar Franci , Mikulic-Petkovsek Maja , Grohar Mariana C. , Hudina Metka , Jakopic Jerneja
Published: July 2023

Black fruits, especially blackberries and black currants, are highly appreciated by consumers due to their nutraceutical properties, which have reported health benefits. This study aimed to assess the fruit quality of the blackberry (cv. ‘Čačanska Bestrna’) and black currant (cv. ‘Čačanska Crna’) created at the Fruit Research Institute, Čačak (Republic of Serbia) by evaluating basic quality parameters (fruit weight, soluble solids), and content of primary (sugars and organic acids) and secondary (phenolic compounds) metabolites. Additionally, the study examined the quality of frozen and long-term frozen storage (6, 9, and 12 months). The results showed that ‘Čačanska Crna’ contained a high level of total soluble solids (15.23°Bx), sugars (93.06 mg g−1), and a high index of sweetness (159.48) as well as a high content of acids (34.35 mg g−1) in the fruit. Both species were found to contain forty-seven phenolic compounds, including phenolic acids, flavanols, and anthocyanins, analyzed using high performance liquid chromatography-mass spectrometry (HPLC-MS). ‘Čačanska Bestrna’ had the highest amounts of phenolic acids (66.85 mg 100 g−1) and flavanols (53.99 mg 100 g−1), whereas ‘Čačanska Crna’ showed the highest levels of flavonols (8.57 mg 100 g−1) and anthocyanins (139.11 mg 100 g−1). Furthermore, the study revealed that anthocyanins were the most abundant phenolic group in both blackberries and black currants, and their levels remained constant during frozen storage. The sugar content in both species remained unchanged, while the acid concentration increased over time in blackberries but remained consistent in black currants. Overall, the findings highlight the superior phenolic content, particularly anthocyanins, of the cultivars with black fruits selected in Serbia. These cultivars have great commercial potential for the fresh market and processing. Moreover, the study suggests that frozen storage is an effective method for preserving their quality and beneficial properties.
Preparation, Characterization, and Application of pH-Response Color-Changeable Films Based on Pullulan, Cooked Amaranth (Amaranthus tricolor L.) Juice, and Bergamot Essential Oil
Authors: Xu Fengfeng , Yun Dawei , Huang Xiaoqian , Sun Bixue , Tang Chao , Liu Jun
Published: July 2023

Pullulan-based smart packaging films were prepared by mixing cooked amaranth juice and bergamot essential oil. The impact of cooked amaranth juice and bergamot essential oil on the color-changeability, structural characterization, and barrier, antioxidant, mechanical and thermal properties of pullulan-based films was determined. Results showed the cooked amaranth juice contained pH-response color-changing betacyanins. The pullulan films containing cooked amaranth juice were color-changeable in pH 9–12 buffers and in ammonia vapor. The color-changeable property of betacyanins in cooked amaranth juice was unaffected by bergamot essential oils. The inner structure of pullulan films was greatly affected by cooked amaranth juice, forming big and ordered humps in film cross-sections. The crystallinity of pullulan films was improved by the combined addition of cooked amaranth juice and bergamot essential oil. Among the films, the pullulan film containing cooked amaranth juice and 6% bergamot essential oil showed the highest UV-vis light barrier property, antioxidant activity, and tensile strength; while the pullulan film containing cooked amaranth juice and 4% bergamot essential oil showed the highest oxygen barrier property and thermal stability. Moreover, the pullulan films containing cooked amaranth juice were able to monitor the freshness of shrimp by presenting color changes from reddish purple to dark red.
Valorisation of Buckwheat By-Product as a Health-Promoting Ingredient Rich in Fibre for the Formulation of Gluten-Free Bread
Authors: Gutiérrez Ángel L. , Villanueva Marina , Rico Daniel , Harasym Joanna , Ronda Felicidad , Martín-Diana Ana Belén , Caballero Pedro A.
Published: July 2023

Bread is a widely consumed food that has often been used as a vehicle for functional ingredients such as dietary fibre. Fibre-rich breads have beneficial physiological effects on health, helping to combat chronic pathologies such as cardiovascular disease, diabetes, and certain types of colon cancer. The aim of this study is to evaluate the technological and nutritional effects of the inclusion of buckwheat hull particles (BH) at two addition levels (3 and 6%) and two particle sizes (fine, D50: 62.7 μm; coarse, D50: 307 μm) in a gluten-free (GF) bread formulation. A significant (p < 0.05) increase in the dough elastic modulus (G’) was observed for all doughs containing BH, from 712 Pa for a rice-based dough to 1027–3738 Pa for those containing BH. Compared to rice-based breads, those containing BH showed a significant (p < 0.05) increase in total dietary fibre content (from three to five times) and in antioxidant capacity (from 78 to 290 mg TE/100 g dw. in the ORAC test). Breads containing fine BH at a level of 3% had similar sensory properties to the rice-based bread, demonstrating that it is possible to improve the TDF content while maintaining the sensory quality of the GF bread.
Effectiveness of Bacteriophages against Biofilm-Forming Shiga-Toxigenic Escherichia coli In Vitro and on Food-Contact Surfaces
Authors: Jaroni Divya , Litt Pushpinder Kaur , Bule Punya , Rumbaugh Kaylee
Published: July 2023

(1) Background: Formation of biofilms on food-contact surfaces by Shiga-toxigenic Escherichia coli (STEC) can pose a significant challenge to the food industry, making conventional control methods insufficient. Targeted use of bacteriophages to disrupt these biofilms could reduce this problem. Previously isolated and characterized bacteriophages (n = 52) were evaluated against STEC biofilms in vitro and on food-contact surfaces. (2) Methods: Phage treatments (9 logs PFU/mL) in phosphate-buffered saline were used individually or as cocktails. Biofilms of STEC (O157, O26, O45, O103, O111, O121, and O145) were formed in 96-well micro-titer plates (7 logs CFU/mL; 24 h) or on stainless steel (SS) and high-density polyethylene (HDPE) coupons (9 logs CFU/cm2; 7 h), followed by phage treatment. Biofilm disruption was measured in vitro at 0, 3, and 6 h as a change in optical density (A595). Coupons were treated with STEC serotype-specific phage-cocktails or a 21-phage cocktail (3 phages/serotype) for 0, 3, 6, and 16 h, and surviving STEC populations were enumerated. (3) Results: Of the 52 phages, 77% showed STEC biofilm disruption in vitro. Serotype-specific phage treatments reduced pathogen population within the biofilms by 1.9–4.1 and 2.3–5.6 logs CFU/cm2, while the 21-phage cocktail reduced it by 4.0 and 4.8 logs CFU/cm2 on SS and HDPE, respectively. (4) Conclusions: Bacteriophages can be used to reduce STEC and their biofilms.
A Feasible Approach to Developing Fiber-Enriched Bread Using Pomegranate Peel Powder: Assessing Its Nutritional Composition and Glycemic Index
Authors: García Paula , Bustamante Andrés , Echeverría Francisca , Encina Cristian , Palma Manuel , Sanhueza Leyla , Sambra Verónica , Pando Maria Elsa , Jiménez Paula
Published: July 2023

The consumption of dietary fiber (DF) has been associated with a reduced incidence of non-communicable diseases. Despite various strategies implemented worldwide to increase DF intake, it remains low. Therefore, the development of new fiber-rich food products that are widely consumed could be a strategy to improve DF intake. In this study, an agro-industrial by-product, pomegranate peel powder (PPP), was used as an innovative source of DF and antioxidant. The objective was to develop a bread enriched with DF, antioxidants, and sensory characteristics by partially replacing wheat flour (WF) with PPP at levels of 0%, 2.5%, 5%, 7.5%, and 10%. Bread with 2.5% and 5% PPP was chosen for a clinical trial to evaluate glycemic response (GR) in healthy subjects and determine the bread’s glycemic index (GI). As the percentage of PPP increased, both the DF and total polyphenol content increased significantly. The highest overall acceptability was achieved with bread containing up to 5% PPP. Consumption of bread with 2.5% and 5.0% PPP significantly reduced the GI compared to the control bread, while the decrease in GR was not significant. PPP could be a potential food and low-cost ingredient to improve the bread’s nutritional quality through its contribution to DF and antioxidants.
Influence of the Amount and Type of Whey Protein on the Chemical, Technological, and Sensory Quality of Pasta and Bakery Products
Authors: Komeroski Marina Rocha , Oliveira Viviani Ruffo de
Published: July 2023

In addition to being an important source of nutrients, pasta and bakery products are consumed globally and so there is a growing need to study them in addition to other ingredients such as whey proteins. These dairy proteins are intended to improve the quality of these foods, as they have important nutritional, technological, and sensory properties that can be exploited. The importance of new formulations in the quality features of pasta and bakery products and gaining an understanding of how the ingredients can interfere with these foods are described. A summary of the latest progress in the application of whey protein in bakery products, as well as their types and quantities from a physicochemical and sensory point of view, is presented. This review was reported following PRISMA recommendations and included articles (n = 32) from scientific journals that evaluated the use of whey protein in bakery products over the past ten years. More than half of the authors (n = 20) used WPC, likely due to its nutritional composition, cost, and easy access. Cake formulations were those with the highest amounts of whey protein, unlike researchers who made bread and pasta, possibly due to the fragility of these preparations. The addition of whey proteins modified the physical characteristics and improved the chemical composition of the bread. However, at higher concentrations (≥30%), they caused damage to the texture characteristics.
Special Issue: Bioceramics, Bioglasses, and Gels for Tissue Engineering
Authors: Dasan Arish , Chandrasekar Ashokraja
Published: July 2023

Undoubtedly, biomaterials such as bioceramics, bioactive glasses, and gels have attracted a wide range of research interest in the field of tissue engineering (TE), as they facilitate the essential support and environment for cells to grow, differentiate, and, specifically, regenerate new tissues [...]
Microemulsion-Based Keratin–Chitosan Gel for Improvement of Skin Permeation/Retention and Activity of Curcumin
Authors: Niu Jiangxiu , Yuan Ming , Gao Panpan , Wang Liye , Qi Yueheng , Chen Jingjing , Bai Kaiyue , Fan Yanli , Liu Xianming
Published: July 2023

Curcumin (Cur) is a kind of polyphenol with a variety of topical pharmacological properties including antioxidant, analgesic and anti-inflammatory activities. However, its low water solubility and poor skin bioavailability limit its effectiveness. In the current study, we aimed to develop microemulsion-based keratin–chitosan gel for the improvement of the topical activity of Cur. The curcumin-loaded microemulsion (CME) was formulated and then loaded into the keratin–chitosan (KCS) gel to form the CME-KCS gel. The formulated CME-KCS gel was evaluated for its characterization, in vitro release, in vitro skin permeation and in vivo activity. The results showed that the developed CME-KCS gel had an orange-yellow and gel-like appearance. The particle size and zeta potential of the CME-KCS gel were 186.45 ± 0.75 nm and 9.42 ± 0.86 mV, respectively. The CME-KCS gel showed desirable viscoelasticity, spreadability, bioadhesion and controlled drug release, which was suitable for topical application. The in vitro skin permeation and retention study showed that the CME-KCS gel had better in vitro skin penetration than the Cur solution and achieved maximum skin drug retention (3.75 ± 0.24 μg/cm2). In vivo experimental results confirmed that the CME-KCS gel was more effective than curcumin-loaded microemulsion (Cur-ME) in analgesic and anti-inflammatory activities. In addition, the CME-KCS gel did not cause any erythema or edema based on a mice skin irritation test. These findings indicated that the developed CME-KCS gel could improve the skin penetration and retention of Cur and could become a promising formulation for topical delivery to treat local diseases.
Controlled Release of Growth Factor from Heparin Embedded Poly(aldehyde guluronate) Hydrogels and Its Effect on Vascularization
Authors: Zhao Yilan , Lin Zezhong , Liu Wenqu , Piao Mingwei , Li Junjie , Zhang Hong
Published: July 2023

To deliver growth factors controllably for tissue regeneration, poly(aldehyde guluronate) (PAG) was obtained from alginate and covalently cross-linked with aminated gelatin (AG) to form PAG/AG hydrogel as a growth factors carrier. The prepared hydrogel exhibits a slow degradation rate and excellent cytocompatibility. Heparin was conjugated with gelatin and embedded into the hydrogel to reserve and stabilize growth factors. Basic fibroblast growth factor (bFGF) was immobilized into the hydrogel and performed sustained release as the hydrogel degraded. The bFGF loaded hydrogel can improve vascularization effectively in a rat dorsal sac model. To summarize, heparin embedded PAG/AG hydrogels would serve as a promising biodegradable vehicle for the controlled delivery of growth factors and promoting vascularization in regenerative medicine.
Injectable Poloxamer Hydrogels for Local Cancer Therapy
Authors: Marques Ana Camila , Costa Paulo Cardoso , Velho Sérgia , Amaral Maria Helena
Published: July 2023

The widespread push to invest in local cancer therapies comes from the need to overcome the limitations of systemic treatment options. In contrast to intravenous administration, local treatments using intratumoral or peritumoral injections are independent of tumor vasculature and allow high concentrations of therapeutic agents to reach the tumor site with minimal systemic toxicity. Injectable biodegradable hydrogels offer a clear advantage over other delivery systems because the former requires no surgical procedures and promotes drug retention at the tumor site. More precisely, in situ gelling systems based on poloxamers have garnered considerable attention due to their thermoresponsive behavior, biocompatibility, ease of preparation, and possible incorporation of different anticancer agents. Therefore, this review focuses on the use of injectable thermoresponsive hydrogels based on poloxamers and their physicochemical and biological characterization. It also includes a summary of these hydrogel applications in local cancer therapies using chemotherapy, phototherapy, immunotherapy, and gene therapy.
Multiple Chromosome Fissions, Including That of the X Chromosome, in Aulacocyclus tricuspis Kaup (Coleoptera, Passalidae) from New Caledonia: Characterization of a Rare but Recurrent Pathway of Chromosome Evolution in Animals
Authors: Dutrillaux Bernard , Dutrillaux Anne-Marie , Salazar Karen , Boucher Stéphane
Published: July 2023

The male karyotype of Aulacocyclus tricuspis Kaup 1868 (Coleoptera, Scarabaeoidea, Passalidae, Aulacocyclinae) from New Caledonia contains an exceptionally high number of chromosomes, almost all of which are acrocentric (53,X1X2Y). Unlike the karyotypes of other species of the pantropical family Passalidae, which are principally composed of metacentric chromosomes, this karyotype is derived by fissions involving almost all the autosomes after breakage in their centromere region. This presupposes the duplication of the centromeres. More surprising is the X chromosome fragmentation. The rarity of X chromosome fission during evolution may be explained by the deleterious effects of alterations to the mechanisms of gene dosage compensation (resulting from the over-expression of the unique X chromosome in male insects). Herein, we propose that its occurrence and persistence were facilitated by (1) the presence of amplified heterochromatin in the X chromosome of Passalidae ancestor, and (2) the capacity of heterochromatin to modulate the regulation of gene expression. In A. tricuspis, we suggest that the portion containing the X proper genes and either a gene-free heterochromatin fragment or a fragment containing a few genes insulated from the peculiar regulation of the X by surrounding heterochromatin were separated by fission. Finally, we show that similar karyotypes with multiple acrocentric autosomes and unusual sex chromosomes rarely occur in species of Coleoptera belonging to the families Vesperidae, Tenebrionidae, and Chrysomelidae. Unlike classical Robertsonian evolution by centric fusion, this pathway of chromosome evolution involving the centric fission of autosomes has rarely been documented in animals.
The Effect of DNA Methylation in the Development and Progression of Chronic Kidney Disease in the General Population: An Epigenome-Wide Association Study Using the Korean Genome and Epidemiology Study Database
Authors: Kim Ji-Eun , Jo Min-Jee , Cho Eunjung , Ahn Shin-Young , Kwon Young-Joo , Gim Jeong-An , Ko Gang-Jee
Published: July 2023

Background: Although knowledge of the genetic factors influencing kidney disease is increasing, epigenetic profiles, which are associated with chronic kidney disease (CKD), have not been fully elucidated. We sought to identify the DNA methylation status of CpG sites associated with reduced kidney function and examine whether the identified CpG sites are associated with CKD development. Method: We analyzed DNA methylation patterns of 440 participants in the Korean Genome and Epidemiology Study (KoGES) with estimated glomerular filtration rates (eGFRs) ≥ 60 mL/min/1.73 m2 at baseline. CKD development was defined as a decrease in the eGFR of <60 at any time during an 8-year follow-up period (“CKD prediction” analysis). In addition, among the 440 participants, 49 participants who underwent a second methylation profiling were assessed for an association between a decline in kidney function and changes in the degree of methylation of CpG sites during the 8 years (“kidney function slope” analysis). Results: In the CKD prediction analysis, methylation profiles of a total of 403,129 CpG sites were evaluated at baseline in 440 participants, and increased and decreased methylation of 268 and 189 CpG sites, respectively, were significantly correlated with the development of CKD in multivariable logistic regression. During kidney function slope analysis using follow-up methylation profiles of 49 participants, the percent methylation changes in 913 CpG sites showed a linear relationship with the percent change in eGFR during 8 years. During functional enrichment analyses for significant CpG sites found in the CKD prediction and kidney function slope analyses, we found that those CpG sites represented MAPK, PI3K/Akt, and Rap1 pathways. In addition, three CpG sites from three genes, NPHS2, CHCHD4, and AHR, were found to be significant in the CKD prediction analysis and related to a decline in kidney function. Conclusion: It is suggested that DNA methylation on specific genes is associated with the development of CKD and the deterioration of kidney function.
Germline CNV Detection through Whole-Exome Sequencing (WES) Data Analysis Enhances Resolution of Rare Genetic Diseases
Authors: Tilemis Faidon-Nikolaos , Marinakis Nikolaos M. , Veltra Danai , Svingou Maria , Kekou Kyriaki , Mitrakos Anastasios , Tzetis Maria , Kosma Konstantina , Makrythanasis Periklis , Traeger-Synodinos Joanne , Sofocleous Christalena
Published: July 2023

Whole-Exome Sequencing (WES) has proven valuable in the characterization of underlying genetic defects in most rare diseases (RDs). Copy Number Variants (CNVs) were initially thought to escape detection. Recent technological advances enabled CNV calling from WES data with the use of accurate and highly sensitive bioinformatic tools. Amongst 920 patients referred for WES, 454 unresolved cases were further analysed using the ExomeDepth algorithm. CNVs were called, evaluated and categorized according to ACMG/ClinGen recommendations. Causative CNVs were identified in 40 patients, increasing the diagnostic yield of WES from 50.7% (466/920) to 55% (506/920). Twenty-two CNVs were available for validation and were all confirmed; of these, five were novel. Implementation of the ExomeDepth tool promoted effective identification of phenotype-relevant and/or novel CNVs. Among the advantages of calling CNVs from WES data, characterization of complex genotypes comprising both CNVs and SNVs minimizes cost and time to final diagnosis, while allowing differentiation between true or false homozygosity, as well as compound heterozygosity of variants in AR genes. The use of a specific algorithm for calling CNVs from WES data enables ancillary detection of different types of causative genetic variants, making WES a critical first-tier diagnostic test for patients with RDs.
Effect of Bovine MEF2A Gene Expression on Proliferation and Apoptosis of Myoblast Cells
Authors: Sun Jinkui , Ruan Yong , Xu Jiali , Shi Pengfei , Xu Houqiang
Published: July 2023

Myocyte enhancer factor 2A (MEF2A) is a member of the myocyte enhancer factor 2 family. MEF2A is widely distributed in various tissues and organs and participates in various physiological processes. This study aimed to investigate the effect of MEF2A expression on the proliferation and apoptosis of bovine myoblasts. CCK8, ELISA, cell cycle, and apoptosis analyses were conducted to assess cell status. In addition, the mRNA expression levels of genes associated with bovine myoblast proliferation and apoptosis were evaluated using RT-qPCR. The results showed that the upregulation of MEF2A mRNA promoted the proliferation rate of myoblasts, shortened the cycle process, and increased the anti-apoptotic rate. Furthermore, the RT-qPCR results showed that the upregulation of MEF2A mRNA significantly increased the cell proliferation factors MyoD1 and IGF1, cell cycle factors CDK2 and CCNA2, and the apoptotic factors Bcl2 and BAD (p < 0.01). These results show that the MEF2A gene can positively regulate myoblast proliferation and anti-apoptosis, providing a basis for the analysis of the regulatory mechanism of the MEF2A gene on bovine growth and development.
Spatial Colinear but Broken Temporal Expression of Duplicated ParaHox Genes in Asexually Reproducing Annelids, Nais communis and Pristina longiseta
Authors: Kostyuchenko Roman P. , Amosov Artem V.
Published: July 2023

ParaHox genes are key developmental regulators involved in the patterning of the digestive tract along the anteroposterior axis and the development of the nervous system. Most studies have focused on the function of these genes in embryogenesis, while their expression patterns in postembryonic development often remain unknown. In this study, we identified for the first time all ParaHox orthologs in two naidid oligochaetes, N. communis and P. longiseta, and described their expression patterns during normal growth and fission in these animals. We showed that Gsx and Cdx are presented by two paralogs, while Xlox is a single copy gene in both species. Using whole-mount in situ hybridization, we also found that orthologs, except for the Xlox gene, have similar activity patterns with minor differences in details, while the expression patterns of paralogs can differ significantly. However, all these genes are involved in axial patterning and/or in tissue remodeling during growth and asexual reproduction in naidids. Moreover, during paratomic fission, these genes are expressed with spatial colinearity but temporal colinearity is broken. The results of this study may be evidence of the functional diversification of duplicated genes and suggest involvement of the ParaHox genes in whole-body patterning during growth and asexual reproduction in annelids.
An Analytical Study on Soil Water Index (SWI), Landslide Prediction and Other Related Factors Using XRAIN Data during the July 2018 Heavy Rain Disasters in Hiroshima, Japan
Authors: Rodrigues Neto José Maria dos Santos , Bhandary Netra Prakash , Fujita Yuichi
Published: July 2023

The rainfall-induced landslide disasters in July 2018 in Southwestern Japan yet again exemplified the severity of slope failure-related damage and the need for improvement of early warning systems. The Japanese Meteorological Agency (JMA) uses a method based on a threshold value of soil water index (SWI), a conceptual measurement that represents saturation of slope soil. The current SWI early warning system uses 60-min rainfall data on a 5-km2 mesh and does not take into consideration other landslide conditioning factors such as slope angle and geology. This study calculates SWI values during the July 2018 disasters in Kure City (Hiroshima Prefecture) using 1-min XRAIN rainfall data in a 250-m mesh to investigate the relationship between SWI and landslide occurrence. It was found that the SWI threshold of 124 mm used in the JMA early warning system for the area was surpassed in all cells. A new SWI threshold calculation method taking slope angle and geology into consideration and produced with machine learning is proposed, comprising power lines for different geological units at a two-dimensional graph where points located above the threshold line represent landslide risk. It is judged that this method would provide a more accurate early warning system for landslide disasters.
Systematic Review of Lung Cancer Screening: Advancements and Strategies for Implementation
Authors: Amicizia Daniela , Piazza Maria Francesca , Marchini Francesca , Astengo Matteo , Grammatico Federico , Battaglini Alberto , Schenone Irene , Sticchi Camilla , Lavieri Rosa , Di Silverio Bruno , Andreoli Giovanni Battista , Ansaldi Filippo
Published: July 2023

Lung cancer is the leading cause of cancer-related deaths in Europe, with low survival rates primarily due to late-stage diagnosis. Early detection can significantly improve survival rates, but lung cancer screening is not currently implemented in Italy. Many countries have implemented lung cancer screening programs for high-risk populations, with studies showing a reduction in mortality. This review aimed to identify key areas for establishing a lung cancer screening program in Italy. A literature search was conducted in October 2022, using the PubMed and Scopus databases. Items of interest included updated evidence, approaches used in other countries, enrollment and eligibility criteria, models, cost-effectiveness studies, and smoking cessation programs. A literature search yielded 61 scientific papers, highlighting the effectiveness of low-dose computed tomography (LDCT) screening in reducing mortality among high-risk populations. The National Lung Screening Trial (NLST) in the United States demonstrated a 20% reduction in lung cancer mortality with LDCT, and other trials confirmed its potential to reduce mortality by up to 39% and detect early-stage cancers. However, false-positive results and associated harm were concerns. Economic evaluations generally supported the cost-effectiveness of LDCT screening, especially when combined with smoking cessation interventions for individuals aged 55 to 75 with a significant smoking history. Implementing a screening program in Italy requires the careful consideration of optimal strategies, population selection, result management, and the integration of smoking cessation. Resource limitations and tailored interventions for subpopulations with low-risk perception and non-adherence rates should be addressed with multidisciplinary expertise.
Perception of Women’s Knowledge of and Attitudes towards Cervical Cancer and Papanicolaou Smear Screenings: A Qualitative Study in South Africa
Authors: Gwavu Zintle , Murray Daphne , Okafor Uchenna Benedine
Published: July 2023

Background: Cervical cancer is the most common form of cancer worldwide. Consequently, it is crucial that women are encouraged to undergo interventions early via Papanicolaou (Pap) smear screenings to improve their health. In light of this, this study explored the knowledge of and attitudes towards cervical cancer and Papanicolaou (Pap) smear screenings among women in the Caleb Motshabi district, South Africa. Four focus group discussions were carried out among 19 women. The interviews/discussion were audio-recorded and transcribed verbatim and then analysed thematically. In this regard, seven main themes emerged that provided insight into the perceptions of the participants regarding their knowledge of and attitudes towards cervical cancer and Papanicolaou (Pap) smear screenings. While the majority of participants were aware of cervical cancer and Pap smears, they lacked more specific knowledge of what this cancer is or its related causes. Although some participants had had a Pap smear done, they neither knew how the procedure was done nor the reasons for it. In addition, most mentioned receiving information about this procedure from their peers rather than healthcare workers. Notably, those with previous experience indicated that they had regular screenings. Furthermore, they better understood exactly how it is done. The findings emphasised women’s limited knowledge of cervical cancer and Pap smears. It further highlighted the need for sustainable education programmes and mobile clinics to encourage an awareness of and accessibility to this particular type of screening within South African communities. Therefore, intervention strategies that make people aware of this specific cancer and encourage the uptake of Papanicolaou (Pap) smear screenings are crucial, as is the continued advocacy for sustained educational programmes and accessible healthcare services.
Experiences of Telenursing in Overcoming Challenges and Applaying Strategies by COVID-19 Patients in Home Isolation: Qualitative Study in Primary Care
Authors: Tort-Nasarre Glòria , Espart Anna , Galbany-Estragués Paola , Álvarez Bruna , Subias-Miquel Martí , Romeu-Labayen Maria
Published: July 2023

During the first wave of the COVID-19 pandemic, there was a significant increase in the use of telenursing to provide care for patients at home. However, the quality of the patient experience when nurses rely on technology instead of personal contact has not been thoroughly investigated. This study aimed to understand the perspectives of COVID-19 patients in home isolation who received telenursing from primary care nurses during the initial phase of the pandemic. A qualitative study was conducted that employed purposive sampling and involved semi-structured interviews via videoconference with fourteen COVID-19 patients from two primary health centers in Catalonia (Spain). Thematic analysis was used, and the study adhered to the COREQ checklist. The findings revealed three themes related to the challenges faced by COVID-19 patients in home isolation: physical symptoms, emotional and social difficulties, and a lack of information. Three themes emerged regarding the strategies patients employed to overcome these challenges and the role of nurses: self-care, emotional support, and personal commitment. The patients reported having achieved strategies to improve their physical, psychological, and situational well-being despite the unprecedented situation. The study highlights that telenursing is a valuable resource for delivering patient-centered care, which could lead to changes in organisational policies and the development of best clinical practices.
Characteristics of Stress and Burnout among Lithuanian University Coaches: A Pre-Pandemic Coronavirus and Post-Pandemic Period Comparison
Authors: Malinauskas Romualdas , Malinauskiene Vilija
Published: July 2023

(1) Background: The majority of studies analyzing associations between burnout, gender, and perceived stress have utilized a cross-sectional design. This present longitudinal study investigated associations between burnout, perceived stress, and gender compared during the pre-pandemic Coronavirus and post-pandemic period among Lithuanian university coaches. (2) Methods: 214 university coaches were randomly selected for the study. Study participants completed two measurements: one pre-pandemic and the second post-pandemic. (3) Results: Gender differences were identified on all burnout elements but not on perceived stress. Repeated measures (RM) multivariate analysis of variance (MANOVA) results did not show a significant effect for the interaction between gender and time. Results of hierarchical (stepwise) regression analyses revealed that perceived stress after the pandemic predicted burnout levels for all three burnout components (exhaustion, depersonalization, and reduced accomplishment) after the pandemic. (4) Conclusions: The current study contributes to the understanding of burnout components in relation to perceived stress through a longitudinal approach using a representative sample of Lithuanian university coaches.
Between Resilience and Agency: A Systematic Review of Protective Factors and Positive Experiences of LGBTQ+ Students
Authors: Fernandes Telmo , Alves Beatriz , Gato Jorge
Published: July 2023

A negative school climate resulting from homophobic and transphobic bias and discrimination is associated with poor well-being and mental health among LGBTQ+ youth. However, protective factors and mechanisms may buffer against the impact of stigmatization. Drawing on the socio-ecological model, minority stress theory, and positive youth development and agency perspectives, we carried out a systematic review of research focusing on factors that can promote the well-being of LGBTQ+ students in educational settings, outlining the primary outcomes from studies published between 2012 and 2022. The PRISMA protocol was used for this review, and 64 articles were scrutinized. The results of the thematic analysis revealed that both external factors (school-inclusive policies and extracurricular activities; social support from school, family, and the community; and school connectedness) and internal factors (psychosocial characteristics and personal agency) promote positive school experiences, such as the exploration of sexual and gender identities in a safe environment. The present findings highlight the need for inclusive school policies and strategies and individual-level interventions that target the well-being and positive mental health outcomes of sexual and gender minority students.