bluuebunny/crossref_metadata_2025 huggingface dataset:
sTNFRII-Fc modification protects human UC-MSCs against apoptosis/autophagy induced by TNF-α and enhances their efficacy in alleviating inflammatory arthritis
Authors: Zhao Yingjie , Yang Xuezhi , Li Siyu , Zhang Bingjie , Li Susu , Wang Xinwei , Wang Yueye , Jia Chengyan , Chang Yan , Wei Wei
Published: July 2021

Background

Tumor necrosis factor (TNF)-α inhibitors represented by etanercept (a fusion protein containing soluble TNF receptor Ⅱ (sTNFRⅡ) and the Fc segment of human IgG1) play a pivotal role in Rheumatoid arthritis (RA) treatment. However, long-term use increases the risk of infection and tumors for their systemic inhibition of TNF-α, which disrupts the regular physiological function of this molecular. Mesenchymal stem cells (MSCs)-based delivery system provides new options for RA treatment with their “homing” and immune-regulation capacities, whereas inflammatory environment (especially TNF-α) is not conducive to MSCs' therapeutic effects by inducing apoptosis/autophagy. Here, we constructed a strain of sTNFRⅡ-Fc-expressing MSCs (sTNFRⅡ-MSC), aiming to offset the deficiency of those two interventions.
Methods

Constructed sTNFRII-Fc lentiviral vector was used to infect human umbilical cord-derived MSCs, and sTNFRII-MSC stable cell line was generated by monoclonal cultivation. In vitro and vivo characteristics of sTNFRII-MSC were assessed by coculture assay and an acute inflammatory model in NOD/SCID mice. The sTNFRII-MSC were transplanted into CIA model, pathological and immunological indicators were detected to evaluate the therapeutic effects of sTNFRII-MSC. The distribution of sTNFRII-MSC was determined by immunofluorescence assay. Apoptosis and autophagy was analyzed by flow cytometry, western blot and immunofluorescence.
Results

sTNFRⅡ-Fc secreted by sTNFRⅡ-MSC present biological activity both in vitro and vivo. sTNFRⅡ-MSC transplantation effectively alleviates mice collagen-induced arthritis (CIA) via migrating to affected area, protecting articular cartilage destruction, modulating immune balance and sTNFRⅡ-MSC showed prolonged internal retention via resisting apoptosis/autophagy induced by TNF-α.
Conclusion

sTNFRⅡ-Fc modification protects MSCs against apoptosis/autophagy induced by TNF-α, in addition to releasing sTNFRⅡ-Fc neutralizing TNF-α to block relevant immune-inflammation cascade, and thus exert better therapeutic effects in alleviating inflammatory arthritis.
Genome-Wide Identification and Expression Profiling Analysis of Wnt Family Genes Affecting Adipocyte Differentiation in Cattle
Authors: Pan Cuili , Wang Shuzhe , Yang Chaoyun , Hu Chunli , Sheng Hui , Xue Xiaoshu , Hu Honghong , Lei Zhaoxiong , Yang Mengli , Ma Yun
Published: July 2021

Wnt is a family of conserved glycoproteins that functions in a variety of crucial biological processes including tissue regeneration, animal development, and cell proliferation and differentiation. For its functional diversity and importance, Wnt gene family has gained considerable research interest in a variety of species. However, comprehensive identification and analysis of Wnt genes in Bovinae is lacking. In this study, we identiﬁed the repertoire of Wnt genes in cattle and seven other species of Bovinae and obtained 19 Wnt genes. Protein properties of these Wnt genes were also described. Phylogenetic analysis showed that the 149 Wnt proteins in Bovinae, together with 38 Wnt proteins from human and mouse, were clustered into 12 major clades. The Wnt genes belonging to the same subfamilies shared similar protein motif compositions and exon-intron patterns. Chromosomal distribution and collinearity analysis of Wnt genes among cattle and five species of Bovinae revealed that this gene family was conservative in evolution. RNA-seq data analysis indicated that Wnt genes exhibited tissue-specific expression patterns in cattle. qPCR analysis of Wnt gene family showed that each gene had a unique expression pattern during bovine adipocytes differentiation. And the comprehensive analysis indicated that Wnt2B may regulate adipose differentiation through activation of FZD5, which is worthy of further study. Our study presents the first genome-wide study of Wnt gene family in Bovinae, and lay the foundation for further functional characterization of the Wnt family in bovine adipocytes differentiation.
Days to Visit an Offshore Island: Effect of Weather Conditions on Arrival Fuel Load and Potential Flight Range for Common Blackbirds Turdus Merula Migrating Over the North Sea
Authors: Kelsey Natalie , Hüppop Ommo , Bairlein Franz
Published: July 2021

Background: Crossing open water instead of following the coast(line) is one way for landbirds to continue migration. However, depending on prevailing weather and the birds’ physiological condition, it is also a risky choice. To date, the question remains as to which interplay between environmental conditions and physiological condition force landbirds to stop on remote islands. We hypothesise that unfavourable winds would affect lean birds with low energy resources, while poor visibility would affect all birds regardless of their fuel load. Methods: To test this hypothesis, we used data of 1312 Common Blackbirds Turdus merula, caught while stopping over on Helgoland during autumn and spring migration. Arrival fuel load was measured using quantitative magnetic resonance technology. Weather parameters (wind and relative humidity as a proxy for visibility) were interpolated for the night before arrival. Further, we calculated whether caught individuals would have managed to successfully cross the North Sea instead of landing on Helgoland, depending on wind conditions. Results: Both wind and relative humidity the night before arrival were correlated with arrival fuel load. After nights with strong headwinds, birds caught the following day were mostly lean, most of which would not have managed to cross the sea if they had not stopped on Helgoland. On the other hand, fat birds that could have successfully travelled on were caught mainly after nights with high relative humidity (≥ 80%). Furthermore, the rate of presumably successful flights was lower due to wind: although only 9% of all blackbirds captured on Helgoland had insufficient fuel loads to allow safe onward migration in still air, real wind conditions would have prevented 30% of birds from successfully crossing the sea during autumn and 21% during spring migration. Conclusions: We were able to decipher how physiological condition, wind and relative humidity force blackbirds to stop on a remote island. Adverse winds tend to affect lean birds with low energy resources, while poor visibility affects all birds, regardless of whether the arrival fuel load was sufficient for onward flight. Our findings will help to understand different migratory strategies and explain further questions like migration timing.
Dendritic cells transfected with a polyepitope DNA construct stimulate an antitumor cytotoxic response in various tumor diseases
Authors: Kurilin Vasily , Kulikova Ekaterina , Shevchenko Julia , Lopatnikova Julia , Obleukhova Irina , Khantakova Julia , Maksyutov Amir , Kuznetsova Maria , Khristin Alexander , Kiryshina Natalya , Kozlov Vadim , Sidorov Sergey , Sokolov Andrey , Vitsin Alexander , Sennikov Sergey
Published: July 2021

Dendritic cells (DCs) loaded with tumor-associated antigens (TAA) are known to be the important agents in antitumor response realization and still figure in lots of treatment schemes in cancer immunotherapy research. Here, we evaluated a cell-based protocol involving the use of original DNA constructs encoding the wide range of TAA epitopes expressed on different epithelial cancers. The constructs were transfected into ex-vivo-generated DCs of cancer patients (breast cancer, colorectal cancer, and non-small cell lung cancer). Direct cytotoxicity assay of effector cells, activated with the transfected DCs, showed a significant increase in the cytotoxicity against autologous tumor cells. The use of DNA-constructs encoding a large number of TAA’s for the in vitro DC loading to activate the T cell response could be a reliable and unified approach for immunotherapy and relapse prevention in patients with epithelial cancers.
Surface Texturing Behavior of Nano-copper Particles under Various Copper Salts System during Copper-assisted Chemical Etching
Authors: Hong Shihao , Ma Liang , Chen Xiuhua , Li Shaoyuan , Ma Wenhui , Chang Yuanchih
Published: July 2021

In this work, the effects of different copper salts on the etching behavior of n-type monocrystalline silicon wafers were detailedly studied by Cu-assisted chemical etching method. Firstly, the inverted pyramid, inverted pyramid-like and oval pit texturing structures were obtained by HF/H2O2/Cu(NO3)2, HF/H2O2/CuSO4 and HF/H2O2/CuCl2 etching systems. Then, the evolution of copper particles deposition behavior was studied to reveal the influencing mechanism of different anion species, the textured wafer surfaces were characterized by scanning electron microscopy (SEM) and ultraviolet-visible (UV) spectrophotometer, the etching rate, silicon wafer thinning and the deposition amount of copper particle was systematically analyzed. We conclude that the binding force between anion and cation, the oxidation of anions and the formation of complex groups [CuCl2]− lead to great difference in the deposition behavior of copper, resulting in different etching morphology and etching rate. The moderate size copper particles deposited from HF/H2O2/Cu(NO3)2 system make that the etching process is mild and the anisotropic etching ability can fully demonstrated, and the regular inverted pyramid structures can be formed under low thinning of silicon wafers. This work will provide guidance for controllable preparation of inverted pyramid structure and future application in high efficiency solar cells.
Utilizing intrapartum transperineal ultrasound in the second stage of labor to predict the difficulty in operative forceps delivery
Authors: Chen Hui , Liang Xuxia , Zhang Shanshan , Nong Zhiwei , Huang Chunli , Ma Yanhua , Liao Shaoxia
Published: July 2021

Objectives:
To assess the predictive value of intrapartum transperineal ultrasound (ITU) measurements, including angle of progression (AOP) and head direction (HD), for the difficulty of forceps delivery in occiput anterior position.
Methods:
This prospective observational study was conducted among 21 nulliparous women with singleton term pregnancies with an indication for forceps delivery. Two operators performed ITU (AOP and HD) immediately before blade application, both at rest and with concurrently contraction and active pushing. Managing obstetricians were blinded to the ultrasound data. A forceps delivery was classified as “complicated” when one or more of the following situations occurred: three or more tractions, a subjective impression of a difficult traction, failed forceps delivery, a third-degree or higher perineal tear and a significant neonatal lesion.
Results:
Of 21 cases, we identified 5 cases of complicated forceps deliveries. AOP at rest (AOP1) was 127.67 ± 11.35° in complicated group and 143.68 ± 15.08° in uncomplicated group (P = 0.043); AOP during contraction (AOP2) was 161.44 ± 9.25° versus 179.55 ± 15.81° (P = 0.026). HD at rest (HD1) was 14.81 ± 15.39° versus 20.30 ± 9.81° (P = 0.351); HD during contraction (HD2) was 26.77 ± 14.99° versus 35.97 ± 10.21° (P = 0.131). By calculating the area under the receiver-operating characteristics curve (AUC), the measurements of AOP1 and AOP2 and their combination (Combined prediction score = -28.790 + 0.105*AOP1(°) + 0.094*AOP2(°)) showed significant predictive values for a safe forceps delivery. The AUCs were 81.3% (95CI 58.4%-94.7%), 83.8% (95 CI 61.3%-96.0%) and 92.5% (95%CI 72.3%-99.4%), respectively. The optimal cut-offs were an AOP1 of 127.8° (sensitivity = 81.25%, specificity = 80%), an AOP2 of 171.4° (sensitivity = 75%, specificity = 100%) and a new combined prediction score of 1.376 (sensitivity = 81.25%, specificity = 80%).
Conclusions:
Using the measurements of AOP1 and AOP2 and their combination can predict a high probability of safe forceps delivery, and such prediction can be helpful for the obstetricians in clinical decision-making.
miR-3065-3p promotes stemness and metastasis by targeting CRLF1 in colorectal cancer
Authors: Li Yi fan , Xun Jing , Wang Bo tao , Ma Yuan , Zhang Lan qiu , Yang Lei , Gao Rui fang , Guan Jun , Liu Tian yu , Gao He jun , Wang Xi mo , Zhang Qi
Published: July 2021

Background: Colorectal cancer is one of the most common malignancy in the world. It has been reported that cancer stem cells (CSCs) serve as the primary drivers of tumorigenesis and tumor progression. There is an urgent need to explore novel molecules that regulate CSCs or their signatures. Increasing evidence has shown that miRNAs are involved in tumorigenesis and progression. Here, we aim to explore the regulatory effect and mechanism of miR-3065-3p on the stemness of colorectal cancer.Methods: The expression of miR-3065-3p in colorectal cancer and the association of miR-3065-3p expression with prognosis of patients with colorectal cancer were analyzed using TCGA dataset or clinical cases. Gain of function in different models, including colorectal cancer cell lines and orthotopic xenograft or liver metastatic mouse model, were used to investigate the effects of miR-3065-3p on colorectal cancer stemness adn metastasis in vitro and in vivo. Cancer stemness was analyzed by detecting the ability of migration and invasion, NANOG, OCT4, and SOX2 expression, ALDH activity and sphere formation. In addition, the interaction of miR-3065-3p and cytokine receptor-like factor 1 (CRLF1) was analyzed theoretically and identified by the luciferase reporter assay. Moreover, the correlation between CRLF1 expression and miR-3065-3p was analyzed in colorectal cancer tissues. Finally, the effect of CRLF1 on the stemness and metastasis of colorectal cancer in vitro and in vivo was assessed.Results: In this report, we found that miR-3065-3p was overexpressed in colorectal cancer and that its high expression was associated with poor prognosis of patients with colorectal cancer. miR-3065-3p promotes the stemness and metastasis of colorectal cancer. Furthermore, CRLF1 was the downstream target of miR-3065-3p and inhibited the stemness of colorectal cancer. In addition, CRLF1 expression was negatively correlated with miR-3065-3p in colorectal cancer tissues. In addition, CRLF1 mediated the effects of miR-3065-3p on promoting stemness-related transcription factor expression in colorectal cancer cells.Conclusion: Our data suggest that miR-3065-3p promoted the stemness and metastasis of colorectal cancer by targeting CRLF1. miR-3065-3p might serve as a promising prognostic marker as well as a therapeutic target for colorectal cancer.
Domain Reconstruction and Mutation Approach to Expand Creative Design Solution Space for Practical Mechanical Systems
Authors: Li Yutong , Wang Yuxin
Published: July 2021

Mechanical systems are fundamental means and carriers for expanding human physical strength. Their novelty is the key factor to determine the cost of manufacture and maintenance, service life, reliability, and other comprehensive performance of products. However, the function-based synthesis methods mainly focus on expanding the solution space in the function layer; each artifact only matches a subfunction individually. Consequently, the obtained physical structures to realize the overall function of design problems are bloated rather than concise. For overcoming this flaw，a computerized creative design system for solving non-routine design problems based on the domain synthesis theory in quotient space was proposed in our previous studies by introducing two bottom-up rule-based matching processes to replace the function-form direct mapping in the FBS model and using form-independent and solution-neutral fine granularity function units to describe the subfunctions. In this paper, the domain reconstruction and mutation processes are introduced to expand the solution space of creative design and increase the creativity of matching physical structures for mechanical systems further. The case study shows that domain reconstruction can effectively imitate human experts' creative design skills to realize the ingenious combination of mechanisms and expand the function-form mapping solution space to the creative solution space. Moreover, the domain mutation can obtain more simplified physical structures to realize the complex top-level functions with few constituent devices. Utilizing fewer and simpler constituent physical structures to meet the functional requirements synergistically determine that the physical structures of mechanical systems figured out by the platform have prominent market competitive advantages due to their structural simplicity and novelty.
Practical Tracking Control of a Class of Uncertain Surface Vessels Under Weak Assumptions on Uncertainties and Reference Trajectories
Authors: Li Jian , Xu Wenqing , Wu Zhaojing , Liu Yungang
Published: July 2021

This paper is devoted to the tracking control of a class of uncertain surface vessels. The main contributions focus on the considerable relaxation of the severe restrictions on system uncertainties and reference trajectory in the related literature. Specifically, all the system parameters are unknown and the disturbance is not necessarily to be differentiable in the paper, but either unknown parameters or disturbance is considered but the other one is excluded in the related literature, or both of them are considered but the disturbance must be continuously differentiable. Moreover, the reference trajectories in the related literature must be at least twice continuously differentiable and themselves as well as their time derivatives must be known for feedback, which are generalized to a more broad class ones that are unknown and only one time continuously differentiable in the paper. To solve the control problem, a novel practical tracking control scheme is presented by using backstepping scheme and adaptive technique, and in turn to derive an adaptive state-feedback controller which guarantees that all the states of the resulting closed-loop system are bounded while the tracking error arrives at and then stay within an arbitrary neighborhood of the origin. Finally, simulation is provided to validate the effectiveness of the proposed theoretical results.
Longitudinal study of a SARS-CoV-2 infection in an immunocompromised patient with X-linked agammaglobulinemia
Authors: Ciuffreda Laura , Lorenzo-Salazar José M. , Alcoba-Florez Julia , Rodriguez-Pérez Héctor , Gil-Campesino Helena , Íñigo-Campos Antonio , de Artola Diego García-Martínez , Valenzuela-Fernández Agustín , Hayek-Peraza Marcelino , Rojo-Alba Susana , Alvarez-Argüelles Marta Elena , Díez-Gil Oscar , González-Montelongo Rafaela , Flores Carlos
Published: July 2021

We describe the case of a 23-year-old immunocompromised male patient with clinically diagnosed X-linked agammaglobulinemia who was admitted to the hospital on the 14th April 2020 due to coronavirus disease of 2019 (COVID-19). Despite COVID-19 test negativizations, the patient was hospitalized most of the time and finally admitted to the intensive care unit where he died from multiorgan failure and shock. Over 149 days, 26 respiratory samples were collected, subjected to viral genome sequencing, and all assigned to the same lineage, supporting a single viral infection event. The accumulation of mutations throughout the course of the infection was accelerated and suggested the presence of compartmentalized viral subpopulations that evolved independently in the upper and lower respiratory airways. These results support that long-term viral shedding in immunocompromised patients is one possible mechanism for the emergence of variants of concern and provide evidence towards the infection control guidelines in these patients.
Tomato Bushy Stunt Virus nanoparticles as a Platform for Drug Delivery to Shh-dependent Medulloblastoma
Authors: Lico Chiara , Tanno Barbara , Marchetti Luca , Novelli Flavia , Giardullo Paola , Arcangeli Caterina , Pazzaglia Simonetta , Podda Maurizio , Santi Luca , Bernini Roberta , Baschieri Selene , Mancuso Mariateresa
Published: July 2021

Background: Medulloblastoma (MB) is a primary central nervous system tumor that affects mainly young children. New strategies of drug delivery are urgent to treat this cancer and, in particular, the SHH-dependent subtype - the most common subgroup in infants - in whom radiotherapy is precluded due to the severe neurological side effects. Plant virus nanoparticles represent an innovative solution to approach this challenge.Methods: Tomato Bushy Stunt virus (TBSV) was functionally characterized using a murine model as carrier for targeted delivery to Shh-MB. The TBSV nanoparticles surface was engineered with peptides described as enabling targeting to brain cancer cells and the modified particles were then produced on large scale using Nicotiana benthamiana plants. Results: Tests on primary cultures of Shh-MB cells and on their cerebellar precursors allowed to define the most efficient peptides able to induce specific uptake of the viral NPs. Immunofluorescence and molecular dynamics simulations supported the hypothesis that the specific targeting of the NPs was mediated by the interaction of the peptides with their natural partners and reinforced by the presentation in association to the viral particle. In vitro experiments demonstrated that the delivery of Doxorubicin through the chimeric TBSV particles allowed to reduce the dose of the chemotherapeutic agent necessary to induce a significant decrease in tumor cells viability. Moreover, the systemic administration of TBSV nanoparticles in MB symptomatic mice confirmed the ability of the virus particles to reach the tumor in a specific manner. A significant advantage in the recognition of the target appeared when TBSV NPs were functionalized with the CooP peptide.Conclusion: Overall, these results open new perspectives for the use of TBSV particles as vehicle for the targeted delivery of chemotherapeutics to MB in order to reduce early and late toxicity.
Legal Factors and Psychological Stress of Health Professionals in the Forensic Area: A Systematic Review Protocol
Authors: Silva João Paulo Nogueira da , Furtado Monalisa Pereira , Magalhães Celina Maria Colino , Pedroso Janari da Silva
Published: July 2021

Background: The illness of mental health workers in the forensic area has multifactorial causes, but among the main ones is the stress resulting from legal factors at work, such as workload; bylaws; wage; promotion; resignation; assignments; safety rules; legislation for the promotion, prevention and protection of health in the workplace; regulation of risky activities, as they significantly vary the health-disease process of health personnel by directly interfering with their work mode and rhythm. Evidence shows that it is the population group more prone to stress, physical and mental exhaustion, and suicide, unlike the rest of the population. It is noteworthy the fact that 90% of the world population suffers from stress, according to the World Health Organization. Given the growth of primary research on the subject and its importance in the various health outcomes, it is necessary to start processes of compilation and synthesis of this evidence to facilitate understanding of the importance of legal factors as stressors to the work routine of health professionals in the forensic area.Methods: The studies included will be original qualitative and quantitative research articles. This systematic review protocol will be conducted in accordance with the Cochrane Manual and will follow PRISMA-P guidelines. Searches will take place from August 2021 to October 2021 and will be performed from the following electronic databases: APA PsycNet, Web of Science, Scopus, PubMed, Oxford Journals and ScienceDirect. Two reviewers will obtain eligible articles, published from 1990 to 2020, to assess the quality of each study and extract data. A narrative and qualitative synthesis will be used to analyze the primary results, and choose the articles relevant to the research objective.Discussion: The results of this review will contribute to a better understanding that stress in the workplace is not restricted to clinical and organizational factors, but also occurs as a result of legal factors. This expanded understanding of the causes of work stress can better support discussions on the reformulation of legislation related to prevention, promotion and recovery of workers' health effectively. Ethics and dissemination: Ethics committee approval is not required for a systematic review protocol. The results will be published in a peer-reviewed health or social science journal.Systematic Review Registration: PROSPERO CRD42021225442
Stigma associated with and attitude regarding mental health help-seeking among undergraduate nursing students in Karachi, Pakistan
Authors: Farooq Salima , Parpio Yasmin , Sattar Saadia , Ali Zahra , Rahim Shirin , Peerwani Ghazal
Published: July 2021

Background
Globally, the prevalence of mental illness amongst university students is a major concern; same is the case with nursing students. Unaddressed mental illness stigma contributes towards hiding of symptoms hampers timely identification of the disease, and leads to reluctance in attitude towards seeking help. This study determined personal and perceived depression stigma and attitude towards help-seeking behaviors and its associated factors, among undergraduate nursing students at a private nursing institution in Karachi, Pakistan.
Methods
We conducted a cross-sectional study by recruiting 246 first and second-Year undergraduate nursing students using consecutive sampling. Data were collected using the Depression Stigma Scale (DSS) and the Attitude toward Seeking Professional Psychological Help Scale (ATSPPHS), along with a demographic questionnaire. Chi-square test, Fischer’s exact test and student T test were used to determine significance of difference of each response between first and second year students. Multiple linear regression was employed to determine predictors of DSS and ATSPPHS. Approval was obtained from the university’s Ethics Review Committee.
Results
The study findings revealed that the mean scores of the personal and perceived stigma scales were 29.7 ± 4.9 and 24.3 ± 6.1, respectively. The mean score of ATSPPHS was 16.5 ± 3.8. The participants reported a mean openness score of7.8 ± 2.6 and a value scale score of 8.7 ± 3.0. A history of psychiatric illness, current living arrangements, and personal depression stigma were found to be significant predictors of ATSPHHS. The year of study was a significant predictor of personal depression stigma whereas both years of study and the current living arrangements were significantly associated with perceived depression stigma.
Conclusion
Attitude towards seeking help for mental illness was significantly influenced by personal and perceived stigma. Moreover, previous history of psychiatric illness and living arrangements also predicted attitudes towards seeking help. In order to encourage positive attitudes towards seeking help for students’ well-being, it is essential to destigmatize mental health issues by adapting context-based, individualized, and group mental health interventions.
Comparative Analysis of Long Non-Coding RNA and mRNA Expression Provides Insights Into Adaptation to Hypoxia in Tibetan Sheep
Authors: Wang Fan , Liu Jianbin , Zeng Qiaoying , Zhuoga Deqing
Published: July 2021

Tibetan sheep have lived on the Qinghai-Tibetan Plateau for thousands of years and they have a good adaptability to the hypoxic environment and strong disease resistance. However, the molecular mechanism of the Tibetan sheep adapting to this extreme environment, especially the role of genetic regulation is still unknown. Emerging evidence suggests that long non-coding RNAs (lncRNAs) participate in the regulation of a diverse range of biological processes. To explore the potential lncRNAs involved in Tibetan sheep adapting to high altitude hypoxia environment, we analyzed the expression profile of lncRNAs and mRNAs in liver and lung tissue of sheep based on the comparative transcriptome analysis between four Tibetan sheep populations (high altitude) and Hu sheep (low altitude). The results showed a total of 7848 differentially expressed (DE) lncRNAs transcripts and 22971 DE mRNAs transcripts were detected by pairwise comparison. The expression patterns of selected mRNAs and lncRNAs were validated by qRT-PCR and the results correlated well with the transcriptome data. Moreover, the functional annotation analysis based on the Gene Ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) databases showed that DE mRNAs and the target genes of the lncRNAs were significantly enriched in organ morphogenesis, response to stimulus, heme binding, immune system, arginine and proline metabolism, and fatty acid biosynthesis. The prediction of mRNA-mRNA and lncRNA-mRNA interaction networks further revealed transcripts potentially involved in adaptation to high altitude hypoxia, the hub genes that DDX24, PDCD11, EIF4A3, NDUFA11, SART1, PRPF8 and TCONS_00306477, TCONS_00306029, TCONS_00139593, TCONS_00293272, TCONS_00313398 were selected. Additionally, a set of target genes, PIK3R1, IGF1R, FZD6, IFNB2, ATF3, MB, CYP2B4, PSMD13, and TGFB1 were also identified as candidate genes associated with high altitude hypoxia adaptation. In conclusion, a collection of aberrantly expressed lncRNA, a set of target genes and biological pathways known to be relevant for altitude adaptation were identified by comparative transcriptome analysis between Tibetan sheep and Hu sheep. Our results first identified the characterization and expression profile of lncRNAs between Tibetan sheep and Hu sheep and provides insights into the genetic regulation mechanisms for Tibetan sheep adaptation to high altitude hypoxia environments.
Lower Tunguska ethnographic expedition of 1930 (based on the personal archive of P.P. Khoroshikh)
Authors:   Institute for Mongolian, Buddhist and Tibetan Studies of Siberian Branch of the Russian Academy of Sciences, Buraeva Olga V. , Buraeva Svetlana V. ,   Institute for Mongolian, Buddhist and Tibetan Studies of Siberian Branch of the Russian Academy of Sciences
Published: July 2021

The article introduces written and visual materials from the center for Oriental Manuscripts and Woodcuts of the Institute of Mongolian, Buddhist and Tibetan Studies of the SB RAS archival collections related to the 1930 expedition to the Evenks of Lower Tunguska. Ethnographers Poltoradnev P.G. and P.P. Khoroshikh from Irkutsk worked as the members of the expedition. In the personal archive of P.P. Khoroshikh a map-scheme of the route, field records about the material, ritual and everyday culture of the Evenks, drafts of articles on the results of the expedition, as well as photographs and drawings with explanations by the author were revealed. The narrative part of the travel diary is compiled in chronological order of visiting camps and localities, contains various details of a complex and long journey. For the first time, the text of travel notes “Under the Arctic circle” is published, compiled in chronological order of visiting camps and localities and revealing various details of a complex and long journey. The subject matter of the identified materials fully reflects both the tasks set for the expedition and the professional interests of the team members – this is an information about the settlement of families, the peculiarities of making homes (chums), boats, utensils, clothing, crafts, rituals, etc. The collected information is supplemented by numerous photos of the region's aborigines, objects and localities, as well as drawings and diagrams of the location of objects with dimensions, orientation to the cardinal directions, location relative to each other, Evenk names and translation into Russian. The authors have formulated the questions, the answers to which have not yet been found in the archive materials – about the future fate of the collected collections, about the actual distribution of the responsibilities in the expedition, about a small number of publications on its results.
Comparing the Intellivent-ASV® Mode with Conventional Ventilation Modes during Weaning after Uncomplicated Cardiac Surgery
Authors: Eremenko А. А. , Komnov R. D. , Titov P. А. , Gerasimenko S. А. , Chakal D. А.
Published: July 2021

The objective: to compare efficacy and safety of Intellivent-ASV® with conventional ventilation modes during weaning in the patients after cardiac surgery.Subjects and methods. In this randomized controlled trial, 40 adult patients were ventilated with conventional ventilation modes and 40 with Intellivent-ASV after uncomplicated cardiac surgery. Eight physicians were involved in the study.Care of both groups was standardized, except for the modes of postoperative ventilation.We compared:- The physician’s workload, through accounting number of manual ventilator settings and time they spent near the ventilator in every group,- Duration of tracheal intubation in ICU,- Evaluation of ventilation safety by considering driving pressure, mechanical power, positive end expiratory pressure, and tidal volume level,- The frequency of adverse events, postoperative complications, and lethality.Results. There were significant differences in the duration of respiratory support in ICU: 226 ± 31 min (Intellivent Group) vs 271 ± 78 min (Control Group) (p = 0.0013).In Intellivent Group, the number of manual ventilator settings and time spent by physicians near the ventilator before tracheal extubation were significantly lower: 0 vs 4 (2–6), and 35 (25–53) sec vs 164 ± 69 sec respectively (p < 0.001 in both cases).Intellivent-ASV provided significantly more protective ventilation through reduction in the driving pressure, tidal volume, FiO2 and PEEP levels but no difference was noted between paO2/FiO2 ratio. ∆P and Vt were significantly lower in Intellivent Group – ∆P on mechanical ventilation was 6 (5–7) cm H2O vs 7.25 (6.5–9.5) cm H2O (p < 0.001); Vt on mechanical ventilation was 6 (5.2–7) vs 7 (6–9.5) ml/kg/PBW (p = 0.000003). PEEP and FiO2 levels were also significantly lower in Intellivent Group, PEEP on mechanical ventilation was 5 (5–7.5) cm H2O vs 7 (5–11.5) cm H2O and FiO2 level was 26 (22–30) % vs 34 (30–40) %.There were no significant differences between the groups in frequency of adverse events and duration of ICU and hospital stay.Conclusion. Application of Intellivent-ASV mode after uncomplicated cardiac surgery provides more protective mechanical ventilation and reduces the physician’s workload without compromising the quality of respiratory support and safety of patients.
Identidad de la universidad franciscana en Colombia. Un estudio en perspectiva pedagógica
Authors: Vanegas Carvajal Edgar Alonso
Published: July 2021

El objetivo de este artículo es mostrar el compromiso de la pedagogía franciscana en la construcción de identidad de la Universidad Franciscana en Colombia. Es decir, se busca indagar acerca de la responsabilidad que tiene la pedagogía franciscana con la identidad en cuatro universidades franciscanas de Colombia. Para ello, se proponen tres escenarios interconectados: se parte de la relación entre franciscanismo y universidad que emerge desde la primera fraternidad franciscana; seguidamente, se analiza la concepción de pedagogía franciscana que declara cada una de las universidades franciscanas de Colombia; finalmente, se proponen líneas y acciones de cooperación interinstitucional entre las universidades franciscanas de Colombia, encaminadas a responder a los retos y desafíos que la globalización le plantea a la universidad moderna.
La investigación es eminentemente documental-descriptiva con enfoque cualitativo de tipo no experimental, puesto que prevaleció un análisis crítico a partir de la revisión y análisis de material bibliográfica seleccionado intencionalmente. En consecuencia, se utilizó como técnica el análisis categorial en los términos propuestos por Corbin y Strauss[1], que parte de un análisis categorial seguido de un estudio comparativo con muestreo teórico.
 
[1] Juliet Corbin and Anselmo Strauss, Basic of qualitative research: Techniques and procedures for developing grounded theory (Newbury Park, California: SAGE Publications, Inc., 2014), 17.
Editorial Franciscanum 176
Authors: Bonilla Morales Jaime Laurence
Published: July 2021

Se muestra una corta reflexión sobre la crisis social y del covid, en relación con la reactivación educativa, así como se muestran cada uno de los artículos de las secciones de filosofía y teología.
Polyphonies of the body in Professional Training. And the wave travels further
Authors: Viafara Sandoval Harold , Pérez Muñoz Mónica Cristina , Parra Hinojosa Sandra
Published: July 2021

"This book is an approach to the sphere of the body from different disciplinary perspectives. For this, a dialogue is necessary, not to unify the concept, but to have the possibility of meeting and moving around different fields of knowledge. This to give meaning to what has been done over time and structure a system of consensus, dissent, and networks in a discourse-practice that has been marginal in the local space. In this encounter, it can be observed that there is no attribute of the body that allows us to speak of it as a whole nor a nature that informs us of a purpose. The body is constructed and this construction refers to a use, a need, and an experience. But on many occasions, its construction has been left aside in some areas of training, without giving it the importance it deserves. I invite the readers to delight in this new wave, in which we can see views from perspectives such as sport, health, disability, education, and art. Winding, undulating movements that have been based on academic reflections, projects, and research by friends and colleagues who have joined these dialogues today and seek to unravel and decant the mysteries of the body, how they perceive it, assume it and it is constitutive of the subject."
Glycemic properties of soursop-based ice cream enriched with moringa leaf powder
Authors: Ademosun Ayokunle O.
Published: July 2021

Introduction. Diabetes is a common disease all over the world that is often a cause of mortality. Ice cream is popular in many countries. However, sugar and fat in its composition makes ice cream a high-caloric product. Soursop (Annona muricata L.) and moringa (Moringa oleifera L.), African medicinal plants, contain natural sugars and are rich in phytochemicals. We aimed to produce ice cream with these plants and evaluate its remedial properties.

Study objects and methods. The study featured ice cream purchased in a local store (control sample) and soursop ice cream with moringa leaf powder (experimental samples). The experimental ice cream samples included ice cream with soursop, ice cream with soursop and 0.1 g of moringa, and ice cream with soursop and 1 g of moringa. The antioxidant properties, glycemic indices, amylose and amylopectin contents, as well as α-amylase and α-glucosidase inhibitory properties of the samples were determined using the standard methods.

Results and discussion. Comparing with the other samples, ice cream with 1 g of moringa showed the highest total phenol and flavonoid contents, ABTS scavenging ability, DPPH radical scavenging ability, hydroxyl scavenging ability, ferric reducing antioxidant properties, and lowest glycemic index. Sensory evaluation revealed a lower overall acceptability of the experimental samples compared to the control ice cream. This could be due a peculiar taste of moringa (the formulation did not include sugar).

Conclusion. Ice cream based on soursop and moringa can be a good alternative to sugar-sweetened ice cream due to its antioxidant properties, low glycemic index, and acceptable sensory attributes.
Continuous hydrolysis of milk proteins in membrane reactors of various configurations
Authors: Ryazantseva Ksenia , Agarkova Eugeniya , Fedotova Olga
Published: July 2021

Introduction. The article provides a review of technologies for membrane fractionation of various hydrolyzed food substrates in membrane bioreactors (MBR). In food industry, MBRs are popular in functional food production, especially in the processing of whey, which is a very promising raw material due to its physicochemical composition.

Study objects and methods. The research was based on a direct validated analysis of scientific publications and featured domestic and foreign experience in MBR hydrolysis of protein raw material.

Results and discussion. The MBR hydrolysis of proteins combines various biocatalytic and membrane processes. This technology makes it possible to intensify the biocatalysis, optimize the use of the enzyme preparation, and regulate the molecular composition of hydrolysis products. The paper reviews MBRs based on batch or continuous stirring, gradient dilution, ceramic capillary, immobilized enzyme, etc. Immobilized enzymes reduce losses that occur during the production of fractionated peptides. Continuous MBRs are the most economically profitable type, as they are based on the difference in molecular weight between the enzyme and the hydrolysis products.

Conclusion. Continuous stirred tank membrane reactors have obvious advantages over other whey processing reactors. They provide prompt separation of hydrolysates with the required biological activity and make it possible to reuse enzymes.
Leadership in Professional Vocabulary Acquisition at ESL classes in Small Groups of Students Majoring in Management
Authors: Dashkina Alexandra
Published: July 2021

The present research featured how learning a foreign language in small groups under the guidance of a student with leadership qualities improves professional vocabulary. Such ESL classes proved useful for students majoring in management. Their work was organized in such a way that students' roles were close to their personal characteristics, i.e. action-, people-, and thinking-oriented. A diagnostic test revealed the students' level of proficiency in English. A psychological training made it possible to identify potential leaders. The students in the experimental group were divided into small subgroups and had to master professional vocabulary under the guidance of the leader. In the control group, the subgroups consisted of either leaders or subordinates. Vocabulary acquisition in the experimental subgroups appeared to be 25 % higher than in the control subgroups. Therefore, teamwork under the guidance of a leader proved quite effective.
Pengaruh Theory Planned Behavior terhadap Keputusan Menabung di Rekening Syariah
Authors:   Schoo lof Business and Economics Universitas Prasetiya Mulya JL. RA. Kartini(TB.Simatupang),Cilandak Barat Jakarta Selatan,Jakarta 12430 Indonesia, irawan Doni , Widhi N Prima ,   Schoo lof Business and Economics Universitas Prasetiya Mulya JL. RA. Kartini(TB.Simatupang),Cilandak Barat Jakarta Selatan,Jakarta 12430 Indonesia, Sagiman Richard ,   School of Business and Economics Universitas Prasetiya MulyaJL. RA. Kartini (TB Simatupang), Cilandak Barat Jakarta Selatan, Jakarta 12430 Indonesia, Nugroho Soesilo ,   School of Business and Economics Universitas Prasetiya MulyaJL. RA. Kartini (TB Simatupang), Cilandak Barat Jakarta Selatan, Jakarta 12430 Indonesia
Published: July 2021

Studi ini menggunakan model Theory Planned Behavior (Icek Ajzen, 1985)dalammenelitikeputusan menabung di rekening Tabungan Syariah.Penelitian dilakukan kepada responden di berbagai wilayah di Indonesia. Dalam penelitian ini terlihat bahwa sikap terhadap perilaku menabung di tabungan, norma subyektifdan persepsi kendali perilaku berpengaruhpositif terhadap intensi menabung di tabungan syariah.Intensi dan perceived behavior controlmempengaruhi secara positif terhadapperilaku menabung di tabungan syariah.Implikasi managerial dari studi ini adalah pentingnya meningkatkan pemahaman masyarakat terhadap kebaikan perbankan syariah serta membuat lebih banyak kemudahan bagi masyarakat untuk menabung di rekening syariah.
Faktor-Faktor yang Memengaruhi Intensi Masyarakat Indonesia untuk Berdonasi pada Kegiatan Crowdfunding
Authors:   School of Business and Economics Universitas Prasetiya Mulya JL. RA. Kartini (TB Simatupang), Cilandak Barat Jakarta Selatan, Jakarta 12430 Indonesia., Adila Iskandar Dea , A.A. Siagian Jerikho ,   School of Business and Economics Universitas Prasetiya Mulya JL. RA. Kartini (TB Simatupang), Cilandak Barat Jakarta Selatan, Jakarta 12430 Indonesia., Clementius Salim Reiner ,   School of Business and Economics Universitas Prasetiya Mulya JL. RA. Kartini (TB Simatupang), Cilandak Barat Jakarta Selatan, Jakarta 12430 Indonesia., Nugraha Kenardi Vincentius ,   School of Business and Economics Universitas Prasetiya Mulya JL. RA. Kartini (TB Simatupang), Cilandak Barat Jakarta Selatan, Jakarta 12430 Indonesia., Adimas Wibisono Yohan ,   School of Business and Economics Universitas Prasetiya Mulya JL. RA. Kartini (TB Simatupang), Cilandak Barat Jakarta Selatan, Jakarta 12430 Indonesia.
Published: July 2021

Indonesia sebagai negara nomor satu yang memiliki penduduk dengan tingkat kedermawanan yang paling tinggi sedunia merupakan landasan yang tepat bagi platform crowdfunding untuk tumbuh. Tujuan dari studi ini adalah untuk mengetahui faktor apa saja yang mempengaruhi intensi seseorang dalam melakukan donasi. Studi ini meneliti hubungan antar orang, sikap terhadap membantu orang lain, inovasi, dan rasa empati sebagai determinan intensi seseorang untuk berpartisipasi dalam ​crowdfunding​. Identifikasi sosial dengan komunitas ​crowdfunding merupakan mediator dalam model penelitian. Hasil dari penelitian ini adalah identifikasi sosial dengan komunitas ​crowdfunding ​dan ​rasa empati memiliki pengaruh yang positif terhadap intensi seseorang dalam melakukan donasi.
Faktor-Faktor yang Mempengaruhi Repurchase Intention dengan Variabel Mediasi Customer Satisfaction pada Network Orchestrator x
Authors:   School of Business and Economics Universitas Prasetiya Mulya JL. RA. Kartini (TB Simatupang), Cilandak Barat Jakarta Selatan, Jakarta 12430 Indonesia., Bakar Adni Abu , Evan Kristian Aloysius ,   School of Business and Economics Universitas Prasetiya Mulya JL. RA. Kartini (TB Simatupang), Cilandak Barat Jakarta Selatan, Jakarta 12430 Indonesia., Asyraf Lufty Iqbal ,   School of Business and Economics Universitas Prasetiya Mulya JL. RA. Kartini (TB Simatupang), Cilandak Barat Jakarta Selatan, Jakarta 12430 Indonesia., Tedy Tedy ,   School of Business and Economics Universitas Prasetiya Mulya JL. RA. Kartini (TB Simatupang), Cilandak Barat Jakarta Selatan, Jakarta 12430 Indonesia.
Published: July 2021

Perkembangan peer-to-peer lodging dimulai dengan kehadiran Airbnb pada tahun 2008 yang tersambung di 192 negara dan telah melayani 60 juta pelancong di seluruh dunia. Kehadiran Airbnb telah memunculkan beberapa model bisnis yang serupa yaitu network orchestrator. Di Indonesia muncul network orchestrator x pada tahun 2015 dengan model bisnis yang mirip hotel tetapi aset berupa gedung dan isinya dimiliki oleh partner/orang ketiga. Network orchestrator x menyediakan sistem aplikasi yang terintegrasi dengan smartphone. Dalam penelitian ini kuesioner disebarkan kepada 401 responden dan jumlah yang dapat digunakan sebanyak 226 kuesioner. Penelitian ini dilakukan dengan menambah dua variabel terhadap model penelitian yang sudah ada yaitu variabel tangible dan intangible, berdasarkan hasil penelitian. Faktor yang secara signifikan mempengaruhi customer satisfaction adalah product performance risk, room and bathroom size, staff’s helpfulness, accuracy of service, personal attention, dan customer satisfaction juga mempengaruhi secara signifikan terhadap repurchase intention.