bluuebunny/crossref_metadata_2025 huggingface dataset:
Mapping and Quantification of Miombo Deforestation in the Lubumbashi Charcoal Production Basin (DR Congo): Spatial Extent and Changes between 1990 and 2022
Authors: Muteya Héritier Khoji , Nghonda Dieu-donné N’Tambwe , Kalenda Franco Mwamba , Strammer Harold , Kankumbi François Munyemba , Malaisse François , Bastin Jean-François , Sikuzani Yannick Useni , Bogaert Jan
Published: September 2023

Population growth in the city of Lubumbashi in the southeastern Democratic Republic of the Congo (DR Congo) is leading to increased energy needs, endangering the balance of the miombo woodland in the rural area referred to as the Lubumbashi charcoal production basin (LCPB). In this study, we quantified the deforestation of the miombo woodland in the LCPB via remote sensing and landscape ecology analysis tools. Thus, the analysis of Landsat images from 1990, 1998, 2008, 2015 and 2022 was supported by the random forest classifier. The results showed that the LCPB lost more than half of its miombo woodland cover between 1990 (77.90%) and 2022 (39.92%) and was converted mainly to wooded savannah (21.68%), grassland (37.26%), agriculture (2.03%) and built-up and bare soil (0.19). Consecutively, grassland became the new dominant land cover in 2022 (40%). Therefore, the deforestation rate (−1.51%) is almost six-times higher than the national average (−0.26%). However, persistent miombo woodland is characterised by a reduction, over time, in its largest patch area and the complexity of its shape. Consequently, because of anthropogenic activities, the dynamics of the landscape pattern are mainly characterised by the attrition of the miombo woodland and the creation of wooded savannah, grassland, agriculture and built-up and bare soil. Thus, it is urgent to develop a forest management plan and find alternatives to energy sources and the sedentarisation of agriculture by supporting local producers to reverse these dynamics.
Opportunities for Monitoring Soil and Land Development to Support United Nations (UN) Sustainable Development Goals (SDGs): A Case Study of the United States of America (USA)
Authors: Mikhailova Elena A. , Zurqani Hamdi A. , Lin Lili , Hao Zhenbang , Post Christopher J. , Schlautman Mark A. , Shepherd George B.
Published: September 2023

Land, including soil resources, makes important contributions to the United Nations (UN) Sustainable Development Goals (SDGs). However, there are challenges in identifying land/soil measurable information (e.g., indicators, metrics, etc.) to monitor the progress toward achieving these goals. This study examines the role of land/soil in selected SDGs (SDG 2: Zero Hunger; SDG 12: Responsible Consumption and Production; SDG 13: Climate Action; SDG 15: Life on Land) and provides practical examples on how to use geospatial analysis to track relevant qualitative and quantitative land/soil data using the contiguous United States of America (USA) as a case study. The innovative aspect of this study leverages geospatial technologies to track the intersection of land use/land cover (LULC) change and soil resources to quantify development trends within the overall land cover matrix to evaluate if these trends are sustainable. Classified land cover data derived from satellite-based remote sensing were used to identify the extent of developed areas in 2016 and the change in development areas since 2011. Most land development through time in the USA has caused losses (area loss of nearly 355,600 km2, with projected midpoint losses of about 5.7 × 1012 kg total soil carbon (TSC) and about $969B (where B = billion = 109, USD) in social costs of carbon dioxide emissions, SC-CO2). All ten soil orders present in the contiguous USA experienced losses from developments, which represents a loss for both biodiversity and soil diversity (pedodiversity). The contiguous USA experienced an increase in land/soil consumption between 2001 and 2016 at the expense of deciduous forest (−3.1%), evergreen forest (−3.0%), emergent herbaceous wetlands (−0.6%), and hay/pasture (−7.9%). These “new” land developments (24,292.2 km2) caused a complete projected midpoint loss of 4.0 × 1011 kg TSC, equivalent to $76.1B SC-CO2. States with the largest developed areas and the highest TSC losses with associated SC-CO2 were Texas and Florida. The proposed methodology used in this study can be applied worldwide, at various spatial scales, to help monitor SDGs over time. With improved tools to monitor SDGs, progress on these SDGs may require linking the SDGs to existing or future international and national legal frameworks.
China’s Investment in the Nigerian Energy Sector: A Prognosis of the Dispute Settlement Paradigm
Authors: Xiang Wen , Oluduro Olubayo
Published: September 2023

Nigeria is one of the top countries of China’s outward foreign direct investments in energy and power projects to meet the needs of China’s fast-growing energy-intensive industries. Following several risks faced by investors to invest in countries with high levels of regulatory, judicial and political uncertainties that appeared in most African states, including Nigeria, contracting parties often take steps to advance and enhance their investment relations and investment climate through an agreement or bilateral investment treaties. This paper examines the China–Nigeria Bilateral Investment Treaty (BIT) and the investment arbitration framework in place in the energy sector. It includes a general analysis on China–African BITs and features common difficulties and possible ways of addressing them. It analyzes the adequacy or otherwise of these frameworks and the various protections afforded to the contracting parties or the host state and the investors. It contends that the current China–Nigeria BIT is lacking essential environmental and social aspects, including sustainable development, corporate social responsibility, transparency and respect for the human rights of host communities, for the promotion of better China–Nigeria investment relations. Notwithstanding the fact that there has not been any known energy dispute in China–Nigeria-related projects, this paper calls for the need for an effective and efficient dispute resolution mechanism to address future disputes between the parties, in order to promote a favorable investment climate for Chinese (and international) investors willing to invest in Nigeria. It advocates that the China–Nigeria BIT should be unambiguous and well drafted to cover issues that could best address investment disputes in the energy sector.
Molecular Insight into Prostate Cancer: Preventive Role of Selective Bioactive Molecules
Authors: Jameel Mohd , Fatma Homa , Nadtochii Liudmila A. , Siddique Hifzur R.
Published: September 2023

Prostate cancer (CaP) is one of the most prevalent male malignancies, accounting for a considerable number of annual mortalities. However, the prompt identification of early-stage CaP often faces delays due to diverse factors, including socioeconomic inequalities. The androgen receptor (AR), in conjunction with various other signaling pathways, exerts a central influence on the genesis, progression, and metastasis of CaP, with androgen deprivation therapy (ADT) serving as the primary therapeutic strategy. Therapeutic modalities encompassing surgery, chemotherapy, hormonal intervention, and radiotherapy have been formulated for addressing early and metastatic CaP. Nonetheless, the heterogeneous tumor microenvironment frequently triggers the activation of signaling pathways, culminating in the emergence of chemoresistance, an aspect to which cancer stem cells (CSCs) notably contribute. Phytochemicals emerge as reservoirs of bioactive agents conferring manifold advantages against human morbidity. Several of these phytochemicals demonstrate potential chemoprotective and chemosensitizing properties against CaP, with selectivity exhibited towards malignant cells while sparing their normal counterparts. In this context, the present review aims to elucidate the intricate molecular underpinnings associated with metastatic CaP development and the acquisition of chemoresistance. Moreover, the contributions of phytochemicals to ameliorating CaP initiation, progression, and chemoresistance are also discussed.
Polymer-Assisted Synthesis, Structure and Magnetic Properties of Bimetallic FeCo- and FeNi/N-Doped Carbon Nanocomposites
Authors: Kugabaeva Gulsara D. , Kydralieva Kamila A. , Bondarenko Lyubov S. , Baimuratova Rose K. , Karpenkov Dmitry Yu. , Golovkova Ekaterina A. , Degtyarenko Pavel N. , Golubeva Nina D. , Uflyand Igor E. , Dzhardimalieva Gulzhian I.
Published: September 2023

Bimetallic FeCo and FeNi nanoparticles attract much attention due to their promising magnetic properties and a wide range of practical applications as recording and storage media, catalytic systems in fuel cells, supercapacitors, lithium batteries, etc. In this paper, we propose an original approach to the preparation of FeCo- and FeNi/N-doped carbon nanocomposites by means of a coupled process of frontal polymerization and thermolysis of molecular co-crystallized acrylamide complexes. The phase composition, structure, and microstructure of the resulting nanocomposites are studied using XRD, IR spectroscopy, elemental and thermal analysis, and electron microscopy data. The main magnetic characteristics of the synthesized nanocomposites, including the field dependences and the ZFC-FC curves peculiarities, are studied. It is shown that the obtained FeCo/N-C nanocomposites exhibit exchange bias behavior at low temperatures. In turn, FeNi/N-C nanocomposites are ferromagnetically ordered.
Some Results in Fuzzy b-Metric Space with b-Triangular Property and Applications to Fredholm Integral Equations and Dynamic Programming
Authors: Mani Gunaseelan , Gnanaprakasam Arul Joseph , Guran Liliana , George Reny , Mitrović Zoran D.
Published: September 2023

In this paper, we introduce the b-triangular property in fuzzy b-metric space. Furthermore, we give some new fixed point results in fuzzy b-metric space for non-continuous mappings. Our results generalize and expand some results from the related literature. Two applications of our results, to solving Fredholm integral equation and in dynamic programming, are also given.
Item Difficulty Prediction Using Item Text Features: Comparison of Predictive Performance across Machine-Learning Algorithms
Authors: Štěpánek Lubomír , Dlouhá Jana , Martinková Patrícia
Published: September 2023

This work presents a comparative analysis of various machine learning (ML) methods for predicting item difficulty in English reading comprehension tests using text features extracted from item wordings. A wide range of ML algorithms are employed within both the supervised regression and the classification tasks, including regularization methods, support vector machines, trees, random forests, back-propagation neural networks, and Naïve Bayes; moreover, the ML algorithms are compared to the performance of domain experts. Using f-fold cross-validation and considering the root mean square error (RMSE) as the performance metric, elastic net outperformed other approaches in a continuous item difficulty prediction. Within classifiers, random forests returned the highest extended predictive accuracy. We demonstrate that the ML algorithms implementing item text features can compete with predictions made by domain experts, and we suggest that they should be used to inform and improve these predictions, especially when item pre-testing is limited or unavailable. Future research is needed to study the performance of the ML algorithms using item text features on different item types and respondent populations.
Anti-Osteoarthritic Effects of Antarctic Krill Oil in Primary Chondrocytes and a Surgical Rat Model of Knee Osteoarthritis
Authors: Ku Sae-Kwang , Kim Jong-Kyu , Chun Yoon-Seok , Song Chang-Hyun
Published: September 2023

Osteoarthritis (OA) is characterized by progressive cartilage destruction and synovitis; however, there are no approved disease-modifying OA drugs. Krill oil (KO) has been reported to possess anti-inflammatory properties and alleviate joint pain in knee OA, indicating its potential to target the inflammatory mechanism of OA. Therefore, the anti-OA effects of KO were investigated in primary chondrocytes and a surgical rat model of knee OA. The oral administration of KO at 200 and 100 mg/kg for 8 weeks improved joint swelling and mobility in the animal model and led to increased bone mineral density and compressive strength in the cartilage. The oral KO doses upregulated chondrogenic genes (type 2 collagen, aggrecan, and Sox9), with inhibition of inflammation markers (5-lipoxygenase and prostaglandin E2) and extracellular matrix (ECM)-degrading enzymes (MMP-2 and MMP-9) in the cartilage and synovium. Consistently, KO treatments increased the viability of chondrocytes exposed to interleukin 1α, accompanied by the upregulation of the chondrogenic genes and the inhibition of the ECM-degrading enzymes. Furthermore, KO demonstrated inhibitory effects on lipopolysaccharide-induced chondrocyte inflammation. Histopathological and immunohistochemical analyses revealed that KO improved joint destruction and synovial inflammation, probably due to the anti-inflammatory, anti-apoptotic, and chondrogenic effects. These findings suggest the therapeutic potential of KO for knee OA.
Recent Advances and Adaptive Strategies in Image Guidance for Cervical Cancer Radiotherapy
Authors: Anghel Beatrice , Serboiu Crenguta , Marinescu Andreea , Taciuc Iulian-Alexandru , Bobirca Florin , Stanescu Anca Daniela
Published: September 2023

The standard of care for locally advanced cervical cancer is external beam radiotherapy (EBRT) with simultaneous chemotherapy followed by an internal radiation boost. New imaging methods such as positron-emission tomography and magnetic resonance imaging have been implemented into daily practice for better tumor delineation in radiotherapy planning. The method of delivering radiation has changed with technical advances in qualitative imaging and treatment delivery. Image-guided radiotherapy (IGRT) plays an important role in minimizing treatment toxicity of pelvic radiation and provides a superior conformality for sparing the organs at risk (OARs) such as bone marrow, bowel, rectum, and bladder. Similarly, three-dimensional image-guided adaptive brachytherapy (3D-IGABT) with computed tomography (CT) or magnetic resonance imaging (MRI) has been reported to improve target coverage and reduce the dose to normal tissues. Brachytherapy is a complementary part of radiotherapy treatment for cervical cancer and, over the past 20 years, 3D-image-based brachytherapy has rapidly evolved and established itself as the gold standard. With new techniques and adaptive treatment in cervical cancer, the concept of personalized medicine is introduced with an enhanced comprehension of the therapeutic index not only in terms of volume (three-dimensional) but during treatment too (four-dimensional). Current data show promising results with integrated IGRT and IGABT in clinical practice and, therefore, better local control and overall survival while reducing treatment-related morbidity. This review gives an overview of the substantial impact that occurred in the progress of image-guided adaptive external beam radiotherapy and brachytherapy.
Acquired Hemophilia A: Bleeding Pattern and Hemostatic Therapeutic Strategies
Authors: Marino Renato
Published: September 2023

Acquired Hemophilia A (AHA) is a rare autoimmune disorder characterized by the onset of a sudden and unexpected bleeding episode in a patient with no personal or family history of bleeding diathesis, and with a typical laboratory feature, i.e., a prolonged activated partial thromboplastin time that is not otherwise explained. This bleeding disorder is caused by autoantibodies directed against the coagulation factor VIII (FVIII). AHA is idiopathic in 50% of cases and is secondary to well-defined diseases in the remaining 50%. AHA affects elderly patients although it has also been observed in the post-partum period. Bleeding manifestations are heterogeneous, ranging from mild to life-threatening bleeds involving limbs and organs. Severe bleeding with a significant decrease in hemoglobin levels must be promptly and adequately treated in order to avoid a worsening of the hemorrhages and their complications. According to international recommendations, the bypass agents (i.e., activated prothrombin complex concentrate and activated recombinant factor VII) and the replacement therapy with recombinant porcine FVIII are considered as the first-line therapy for bleeding control, due to their proven clinical efficacy. Plasma-derived or recombinant FVIII concentrates could be used as second-line treatments. Emicizumab may represent a valid and interesting therapeutic option for prophylaxis of bleeding recurrences.
Utilizing a Combination of Network Pharmacology and Experimental Validation to Unravel the Mechanism by Which Kuanxiongzhuyu Decoction Ameliorates Myocardial Infarction Damage
Authors: Wu Yihao , Li Miaofu , Chen Liuying , Xu Linhao , Xu Yizhou , Zhong Yigang
Published: September 2023

Background and Objectives: With the growing incidence and disability associated with myocardial infarction (MI), there is an increasing focus on cardiac rehabilitation post-MI. Kuanxiongzhuyu decoction (KXZY), a traditional Chinese herbal formula, has been used in the rehabilitation of patients after MI. However, the chemical composition, protective effects, and underlying mechanism of KXZY remain unclear. Materials and Methods: In this study, the compounds in KXZY were identified using a high-performance liquid chromatography-mass spectrometry (HPLC-MS) analytical method. Based on the compounds identified in the KXZY, we predictively selected the potential targets of MI and then constructed a protein–protein interaction (PPI) network to identify the key targets. Furthermore, the DAVID database was used for the GO and KEGG analyses, and molecular docking was used to verify the key targets. Finally, the cardioprotective effects and mechanism of KXZY were investigated in post-MI mice. Results: A total of 193 chemical compounds of KXZY were identified by HPLC-MS. In total, 228 potential targets were obtained by the prediction analysis. The functional enrichment studies and PPI network showed that the targets were largely associated with AKT-pathway-related apoptosis. The molecular docking verified that isoguanosine and adenosine exhibited excellent binding to the AKT. In vivo, KXZY significantly alleviated cardiac dysfunction and suppressed AKT phosphorylation. Furthermore, KXZY significantly increased the expression of the antiapoptotic proteins Bcl-2 and Bcl-xl and decreased the expression of the proapoptotic protein BAD. Conclusions: In conclusion, the network pharmacological and experimental evidence suggests that KXZY manifests anti-cardiac dysfunction behavior by alleviating cardiomyocyte apoptosis via the AKT pathway in MI and, thus, holds promising therapeutic potential.
Theoretical and Experimental Analysis of Osmotically Assisted Reverse Osmosis for Minimum Liquid Discharge
Authors: Ju Jaehyun , Lee Seoyeon , Kim Yusik , Cho Hyeongrak , Lee Sangho
Published: September 2023

Osmotically assisted reverse osmosis (OARO) is an innovative process that shows promising potential in the treatment of brine produced by conventional reverse osmosis (RO) systems. This study presents a theoretical and experimental analysis of the OARO process, focusing on its application to achieve minimum liquid discharge (MLD). This theoretical analysis includes the development of a mathematical model to describe the transport phenomena occurring during OARO. By considering mass balance equations coupled with transport equations, the theoretical model allows for the simulation of a full-scale system consisting of a single-stage RO and a four-stage OARO. Experimental investigations are also conducted to validate the theoretical model and to evaluate the performance of the OARO process. A laboratory-scale OARO system is designed and operated using a synthetic RO brine. Various operating conditions, including applied pressure, feed concentration, and draw concentration, are varied to investigate their effects on process performance. The experimental results demonstrate the feasibility of OARO as an MLD solution and also validate the predictions of the theoretical model, confirming its reliability for process optimization and design. The results of the theoretical analysis show that OARO has the potential to significantly improve water recovery compared to conventional RO. Based on the simulation, the optimal operating conditions are explored, leading to a significant reduction (up to 89%) in the volume of brine discharge.
Scalability of Sartobind® Rapid A Membrane for High Productivity Monoclonal Antibody Capture
Authors: Yang Sabrina , Braczkowski Ryszard , Chen Shih-Hsun , Busse Ricarda , Li Yudhi , Fabri Louis , Bekard Innocent Berbelle
Published: September 2023

Improved upstream titres in therapeutic monoclonal antibody (mAb) production have shifted capacity constraints to the downstream process. The consideration of membrane-based chromatographic devices as a debottlenecking option is gaining increasing attention with the recent introduction of high-capacity bind and elute membranes. We have evaluated the performance and scalability of the Sartobind® Rapid A affinity membrane (1 mL) for high-productivity mAb capture. For scalability assessment, a 75 mL prototype device was used to process 100 L of clarified cell culture harvest (CH) on a novel multi-use rapid cycling chromatography system (MU-RCC). MabSelect™ PrismA (4.7 mL) was used as a benchmark comparator for Protein A (ProtA) resin studies. Results show that in addition to a productivity gain of >10×, process and product quality attributes were either improved or comparable to the benchmark. Concentrations of eluate pools were 7.5× less than that of the benchmark, with the comparatively higher bulk volume likely to cause handling challenges at process scale. The MU-RCC system is capable of membrane operation at pilot scale with comparable product quality profile to the 1 mL device. The Sartobind® Rapid A membrane is a scalable alternative to conventional ProtA resin chromatography for the isolation and purification of mAbs from harvested cell culture media.
The Membrane-Mediated Interaction of Liquid-Ordered Lipid Domains in the Presence of Amphipathic Peptides
Authors: Pinigin Konstantin V. , Akimov Sergey A.
Published: September 2023

The lipid membranes of living cells are composed of a large number of lipid types and can undergo phase separation with the formation of nanometer-scale liquid-ordered lipid domains, also called rafts. Raft coalescence, i.e., the fusion of lipid domains, is involved in important cell processes, such as signaling and trafficking. In this work, within the framework of the theory of elasticity of lipid membranes, we explore how amphipathic peptides adsorbed on lipid membranes may affect the domain–domain fusion processes. We show that the elastic deformations of lipid membranes drive amphipathic peptides to the boundary of lipid domains, which leads to an increase in the average energy barrier of the domain–domain fusion, even if the surface concentration of amphipathic peptides is low and the domain boundaries are only partially occupied by the peptides. This inhibition of the fusion of lipid domains may lead to negative side effects of using amphipathic peptides as antimicrobial agents.
The Role of Indoor Microbiome and Metabolites in Shaping Children’s Nasal and Oral Microbiota: A Pilot Multi-Omic Analysis
Authors: Zhang Mei , Tang Hao , Yuan Yiwen , Ou Zheyuan , Chen Zhuoru , Xu Yanyi , Fu Xi , Zhao Zhuohui , Sun Yu
Published: September 2023

Maintaining a diverse and well-balanced nasal and oral microbiota is vital for human health. However, the impact of indoor microbiome and metabolites on nasal and oral microbiota remains largely unknown. Fifty-six children in Shanghai were surveyed to complete a questionnaire about their personal and environmental characteristics. The indoor microbiome and metabolites from vacuumed indoor dust were profiled via shotgun metagenomics and untargeted liquid chromatography-mass spectrometry (LC–MS). The nasal and oral microbiota in children was characterized using full-length 16S rRNA sequencing from PacBio. Associations between personal/environmental characteristics and the nasal/oral microbiota were calculated using PERMANOVA and regression analyses. We identified 6247, 431, and 342 microbial species in the indoor dust, nasal, and oral cavities, respectively. The overall nasal and oral microbial composition showed significant associations with environmental tobacco smoke (ETS) exposure during pregnancy and early childhood (p = 0.005 and 0.03, respectively), and the abundance of total indoor flavonoids and two mycotoxins (deoxynivalenol and nivalenol) (p = 0.01, 0.02, and 0.03, respectively). Notably, the abundance of several flavonoids, such as baicalein, eupatilin, isoliquiritigenin, tangeritin, and hesperidin, showed positive correlations with alpha diversity and the abundance of protective microbial taxa in nasal and oral cavities (p < 0.02), suggesting their potential beneficial roles in promoting nasal/oral health. Conversely, high carbohydrate/fat food intake and ETS exposure diminished protective microorganisms while augmenting risky microorganisms in the nasal/oral cavities. Further, potential microbial transfer was observed from the indoor environment to the childhood oral cavity (Moraxella catarrhalis, Streptococcus mitis, and Streptococcus salivarius), which could potentially increase virulence factors related to adherence and immune modulation and vancomycin resistance genes in children. This is the first study to reveal the association between the indoor microbiome/metabolites and nasal/oral microbiota using multi-omic approaches. These findings reveal potential protective and risk factors related to the indoor microbial environment.
Biocide Syntheses Bee Venom-Conjugated ZnO@αFe2O3 Nanoflowers as an Advanced Platform Targeting Multidrug-Resistant Fecal Coliform Bacteria Biofilm Isolated from Treated Wastewater
Authors: Sharaf Mohamed , Mohammed Eman Jassim , Farahat Eman M. , Alrehaili Amani A. , Alkhudhayri Abdulsalam , Ali Ahmed Mohamed , Zahra Abdullah A. , Zakai Shadi A. , Elkelish Amr , AlHarbi Maha , Saad Mai Farag
Published: September 2023

This study targeted developing a novel Zinc oxide with alpha hematite nanoflowers (NFs)-loaded bee venom (Bv) (Bv-ZnO@αFe2O3 NFs) as a bio-natural product from bees to combine both the advantages of combination magnetic properties and the antimicrobial and anti-biofilm properties on isolated coliform bacteria from the effluent of wastewater treatment plants. About 24 isolates of treated wastewater isolates were multidrug resistant (MDR). The phylogenetic grouping of Escherichia coli (E. coli) and Klebsiella pneumonia (K. pneumonia) showed that the largest group was Group A, followed by Group B2 and Group B1. Fourier transform infrared (FTIR), The X-ray diffraction (XRD), and scanning electron microscopy-energy dispersive X-ray analysis (SEM− EDX) validated the coating operation’s contact with Bv onto ZnO@αFe2O3 NFs. According to high-resolution transmission electron microscopy (TEM) and selected area electron diffraction (SAED), pattern analyses for prepared nanoformulations exhibited a spherical shape of αFe2O3 (~9–15 nm), and floral needle shapes with uniform distribution of size with aggregation of ZnOαFe2O3 and Bv-ZnO@αFe2O3 NFs around (~100–200 nm). The toxicity of Bv-ZnO@αFe2O3 NFs was comparable up to 125 µg mL−1, when it reached 64.79% (IC50, 107.18 µg mL−1). The antibacterial activity showed different zones of inhibition against different isolates. The biofilm inhibitory activity of NPs and NFs showed a highly significant reduction (p < 0.001) in treated biofilms with ZnO@αFe2O3 and Bv-ZnO@αFe2O3. In essence, ZnO@αFe2O3 and Bv-ZnO@αFe2O3 NFs are promising antimicrobials for inhibiting the growth and biofilm of MDR E. coli and K. pneumonia isolates, thereby, biocontrol of wastewater.
SARS-CoV-2 Rapid Antigen Test Based on a New Anti-Nucleocapsid Protein Monoclonal Antibody: Development and Real-Time Validation
Authors: Coelho Fabiana Fioravante , da Silva Miriam Aparecida , Lopes Thiciany Blener , Polatto Juliana Moutinho , de Castro Natália Salazar , Andrade Luis Adan Flores , Lourenço Karine Lima , Sato Hugo Itaru , de Carvalho Alex Fiorini , Coelho Helena Perez , Bagno Flávia Fonseca , Luz Daniela , Viala Vincent Louis , Cattony Pedro Queiroz , Melo Bruna de Sousa , Moro Ana Maria , Quintilio Wagner , Barbosa Ana Paula , Bomfim Camila Gasque , Soares Camila Pereira , Guzzo Cristiane Rodrigues , Fonseca Flavio Guimarães , Durigon Edison Luiz , Gazzinelli Ricardo Tostes , Ribeiro Teixeira Santuza M. , Piazza Roxane Maria Fontes , Fernandes Ana Paula
Published: September 2023

SARS-CoV-2 diagnostic tests have become an important tool for pandemic control. Among the alternatives for COVID-19 diagnosis, antigen rapid diagnostic tests (Ag-RDT) are very convenient and widely used. However, as SARS-CoV-2 variants may continuously emerge, the replacement of tests and reagents may be required to maintain the sensitivity of Ag-RDTs. Here, we describe the development and validation of an Ag-RDT during an outbreak of the Omicron variant, including the characterization of a new monoclonal antibody (anti-DTC-N 1B3 mAb) that recognizes the Nucleocapsid protein (N). The anti-DTC-N 1B3 mAb recognized the sequence TFPPTEPKKDKKK located at the C-terminus of the N protein of main SARS-CoV-2 variants of concern. Accordingly, the Ag-RDT prototypes using the anti-DTC-N 1B3 mAB detected all the SARS-CoV-2 variants—Wuhan, Alpha, Gamma, Delta, P2 and Omicron. The performance of the best prototype (sensitivity of 95.2% for samples with Ct ≤ 25; specificity of 98.3% and overall accuracy of 85.0%) met the WHO recommendations. Moreover, results from a patients’ follow-up study indicated that, if performed within the first three days after onset of symptoms, the Ag-RDT displayed 100% sensitivity. Thus, the new mAb and the Ag-RDT developed herein may constitute alternative tools for COVID-19 point-of-care diagnosis and epidemiological surveillance.
Re-Evaluation of the Cross-Reactions of the Antibody against the Causative Agent for Paracoccidioidomycosis Ceti; Paracoccidioides ceti and the Related Fungal Species
Authors: Kanegae Hikaru , de Souza Suguiura Igor Massahiro , Tashiro Rentaro , Konno Toshihiro , Hou De-Xing , Sano Ayako , Eto Takeshi , Ueda Keiichi , Hossain Md. Amzad
Published: September 2023

Paracoccidioidomycosis ceti (PCM-C) is a chronic granulomatous keloidal dermatitis in cetaceans that has been reported worldwide and is caused by Paracoccidioides ceti. Serological cross-reactions among highly pathogenic fungal infections and related diseases have been reported. However, the true cross-reaction of antibodies against P. ceti has remained unknown due to the use of positive control sera from infected dolphins. This study aimed to re-evaluate antibodies from mechanically dislodged fungal cells in the infected tissue of a PCM-C case and demonstrate the actual cross-reaction. The results revealed a limited cross-reaction between PCM-C and paracoccidioidomycosis, while the antibodies did not react with other pathogens such as Coccidioides posadasii, Histoplasama capsulatum, and Arthrographis kalrae. Thus, the method for evaluation of the antibody against PCM-C is reliable, and there is potential for epidemiological study.
Epidemiological, Genetic, and Phenotypic Characteristics of Non-Typhoidal Salmonella in Young Children, as Obtained from a Tertiary Hospital in Guangzhou, China
Authors: Gong Baiyan , Feng Yulian , Zhuo Zhenxu , Song Jingjie , Chen Xiankai , Li Xiaoyan
Published: September 2023

Gastroenteritis caused by non-typhoidal Salmonella (NTS) is a significant disease in childhood, ranking as the seventh-leading cause of diarrhea mortality in children aged < 5 years. To understand the epidemiological, genetic, and phenotypic characteristics of NTS, 465 anal swabs from children aged < 5 years in a tertiary hospital in Conghua District, Guangzhou, China, were collected from June to October 2021. An average prevalence of 35.27% (164/465) was observed, with whole genome sequencing identifying 11 serotypes, among which Salmonella 1,4,[5],12:i:- was the most prevalent (65.24%, 107/164). Meanwhile, ST34 was found to be the predominant subtype. Children who are breastfed, eat fresh food, and have good hygiene habits show a relatively low prevalence of NTS. Fever is a common symptom that may be caused by NTS infection. Antimicrobial resistance testing revealed that the majority of strains were resistant to tetracycline (83.5%) and ampicillin (82.3%), with multi-drug resistance (MDR) observed in 50.61% (83/164) of all strains tested. The predominant resistance spectrum presents as tetracycline-ampicillin-chloramphenicol-trimethoprim-sulfamethoxazole (30.49%, 50/164). The antimicrobial resistance rates (2.4%, 9.8%, 9.8%, 10.4%, 9.1%, and 3.7%, respectively) of cephalosporins (cefepime, cefuroxime, cefuroxime axetil, ceftriaxone, ceftazidime, and cefoxitin) were low. Therefore, continued surveillance of the prevalence and MDR profiles of NTS, along with the rational use antibiotics, is required. This protocol is significant for preventing further dissemination of NTS and formulating effective prevention and control strategies.
Sedimentary Environments and Paleoclimate Control of the Middle Miocene Balikpapan Group, Lower Kutai Basin (Indonesia): Implications for Evaluation of the Hydrocarbon Potential
Authors: Jamaluddin  , Wagreich Michael , Gier Susanne , Schöpfer Kateřina , Battu Desianto Payung
Published: September 2023

Sedimentary organic matter concentrated in source rocks forms the main source for the formation of hydrocarbons. Its deposition and preservation are strongly controlled by the depositional environment and paleoclimate. This study evaluates the paleoenvironment and the paleoclimatic controls of sediments in the Middle Miocene Balikpapan Group, Mahakam Delta of the Lower Kutai Basin, Indonesia. The sedimentary succession of the Mentawir Formation, encountered in three wells (MHK 1, MHK 3, and MHK 4), contains interbedded sandstones, siltstones, shales, and coal. Gamma ray log analysis has revealed four facies associations: (a) funnel-, (b) bell-, (c) cylindrical-, and (d) bow-shaped patterns, which, together with sedimentological and mineralogical analysis, suggest a fluvio-deltaic depositional environment during the Middle Miocene in the study area. Sedimentary successions from wells MHK 1 and MHK 3 comprise interbedded sandstone and siltstones and are interpreted to represent repeatedly occurring delta plain, delta front, and prodelta deposits. The succession encountered in well MHK 4 mostly consists of amalgamated sandstones and indicates a predominantly fluvial to upper delta plain environment with distributary channels and crevasse splays interbedded with only thin delta front deposits. X-ray diffraction–clay fraction analysis shows that the <2 μm clay-sized fraction consists of kaolinite (38%–67%), illite (14%–29%), chlorite (2%–17%), and mixed-layer illite/smectite (I/S) (14%–30%). Kaolinite formation and abundance indicates a hinterland climate classified as type Af (tropical rainforest) and intensive chemical weathering conditions in the source areas related to tropical to sub-tropical climates with high precipitation. Under such climatic conditions, kaolinite and I/S mixed-layer minerals are preferentially formed because the characteristic ions, K+, Na+, Ca2+, and Fe2+, are leached away. Thus, the production, transport, distribution, and preservation of sedimentary organic matter in the onshore Mentawir Formation of the Balikpapan Group are predominantly controlled by the humid tropical climate and fluvio-deltaic processes.
On the Prediction of the Mechanical Properties of Limestone Calcined Clay Cement: A Random Forest Approach Tailored to Cement Chemistry
Authors: Han Taihao , Aylas-Paredes Bryan K. , Huang Jie , Goel Ashutosh , Neithalath Narayanan , Kumar Aditya
Published: September 2023

Limestone calcined clay cement (LC3) is a sustainable alternative to ordinary Portland cement, capable of reducing the binder’s carbon footprint by 40% while satisfying all key performance metrics. The inherent compositional heterogeneity in select components of LC3, combined with their convoluted chemical interactions, poses challenges to conventional analytical models when predicting mechanical properties. Although some studies have employed machine learning (ML) to predict the mechanical properties of LC3, many have overlooked the pivotal role of feature selection. Proper feature selection not only refines and simplifies the structure of ML models but also enhances these models’ prediction performance and interpretability. This research harnesses the power of the random forest (RF) model to predict the compressive strength of LC3. Three feature reduction methods—Pearson correlation, SHapley Additive exPlanations, and variable importance—are employed to analyze the influence of LC3 components and mixture design on compressive strength. Practical guidelines for utilizing these methods on cementitious materials are elucidated. Through the rigorous screening of insignificant variables from the database, the RF model conserves computational resources while also producing high-fidelity predictions. Additionally, a feature enhancement method is utilized, consolidating numerous input variables into a singular feature while feeding the RF model with richer information, resulting in a substantial improvement in prediction accuracy. Overall, this study provides a novel pathway to apply ML to LC3, emphasizing the need to tailor ML models to cement chemistry rather than employing them generically.
Land Reclamation Using Typical Coal Gasification Slag in Xinjiang: A Full-Cycle Environmental Risk Study
Authors: Zhang Kai , Song Shuang , Zhao Jiangang , Li Xiaonan , Liu Changyong
Published: September 2023

A rising quantity of coal gasification slag (CGS) is produced annually. Land reclamation is a valuable method for efficiently utilizing coal gasification slag on a large scale. The ecological influence of CGS during land reclamation has not been widely investigated. This article covers the entire CGS use cycle for land reclamation, which includes generation, storage, and disposal. The environmental risk of using CGS for land reclamation was assessed by combining four environmental risk assessment methods. The results show no environmental risk for coal gasification coarse slag (CGCS) and coal gasification fine slag (CGFS) at the generation and storage stages. However, a concern remains regarding manganese leaching from CGCS during the storage stage. In the disposal phase, no environmental risk is present when up to 15% of CGCS and CGFS are applied to land reclamation projects. However, the environmental risk of disposing of 100% of CGS in a landfill cannot be disregarded. Conversely, the full-cycle use of CGS for land reclamation carries no environmental risk.
Zinc Hydroxystannate/Carbon Nanotube Hybrids as Flame Retardant and Smoke Suppressant for Epoxy Resins
Authors: Shi Congling , Wan Mei , Qian Xiaodong , Jing Jingyun , Zhou Keqing
Published: September 2023

Novel hybrid flame retardants containing zinc hydroxystannate and carbon nanotubes (ZHS-CNTs) were synthesized using the coprecipitation method, and the structure and morphology of ZHS−CNTs were investigate using an X-ray powder diffractometer (XRD), scanning electron microscope (SEM), transmission electron microscopy (TEM) and thermogravimetric analyzer (TGA). Then, the ZHS, CNTs and ZHS−CNTs were incorporated into EP, respectively, and the flame-retardant and smoke inhibition performance of the composites were compared and studied. Among the three composites, the EP/ZHS-CNT composites have the highest improvements on the fire resistance and smoke inhibition properties. With only 2.0 wt.% ZHS-CNT hybrids, the pHRR of EP/ZHS-CNT composite materials is reduced by 34.2% compared with EP. Moreover, the release of toxic gases including CO, CO2 and SPR from the composites was also effectively inhibited. The mechanisms of flame retardant and smoke inhibition were investigated and the improved properties were generally ascribed to the synergistic flame-retardant effects between ZHS and CNTs, the catalyzing effect of ZHS and the stable network structure of CNTs.
Comparison of H2O Adsorption and Dissociation Behaviors on Rutile (110) and Anatase (101) Surfaces Based on ReaxFF Molecular Dynamics Simulation
Authors: Zhou He , Zhang Heng , Yuan Shiling
Published: September 2023

The relationship between structure and reactivity plays a dominant role in water dissociation on the various TiO2 crystallines. To observe the adsorption and dissociation behavior of H2O, the reaction force field (ReaxFF) is used to investigate the dynamic behavior of H2O on rutile (110) and anatase (101) surfaces in an aqueous environment. Simulation results show that there is a direct proton transfer between the adsorbed H2O (H2Oad) and the bridging oxygen (Obr) on the rutile (110) surface. Compared with that on the rutile (110) surface, an indirect proton transfer occurs on the anatase (101) surface along the H-bond network from the second layer of water. This different mechanism of water dissociation is determined by the distance between the 5-fold coordinated Ti (Ti5c) and Obr of the rutile and anatase TiO2 surfaces, resulting in the direct or indirect proton transfer. Additionally, the hydrogen bond (H-bond) network plays a crucial role in the adsorption and dissociation of H2O on the TiO2 surface. To describe interfacial water structures between TiO2 and bulk water, the double-layer model is proposed. The first layer is the dissociated H2O on the rutile (110) and anatase (101) surfaces. The second layer forms an ordered water structure adsorbed to the surface Obr or terminal OH group through strong hydrogen bonding (H-bonding). Affected by the H-bond network, the H2O dissociation on the rutile (110) surface is inhibited but that on the anatase (101) surface is promoted.
Microwave Heating for Synthesis of Carbonaceous Adsorbents for Removal of Toxic Organic and Inorganic Contaminants
Authors: Bazan-Wozniak Aleksandra , Machelak Katarzyna , Nosal-Wiercińska Agnieszka , Pietrzak Robert
Published: September 2023

The residues obtained from the extraction of Inonotus obliquus fungus were used to produce carbonaceous adsorbents. The initial material was subjected to pyrolysis in a microwave oven. The adsorbents were characterized through elemental analysis, low-temperature nitrogen adsorption/desorption isotherms, and Boehm titration. The carbonaceous adsorbents were tested for the removal of NO2, methylene blue, and malachite green. The results indicated that the obtained carbonaceous adsorbents exhibited basic characteristics and possessed specific surface areas of 372 and 502 m2/g. The adsorption process of liquid contaminants was modeled using the single-layer Langmuir model. The maximum adsorption capacities were found to be 101 and 109 mg/g for methylene blue, and 75 and 77 mg/g for malachite green. The kinetic study demonstrated that the adsorption of methylene blue and malachite green was better described by a pseudo-second order model. The study affirmed that the adsorption of organic dyes onto the resultant carbonaceous adsorbents was both spontaneous and endothermic. The study also demonstrated that the presence of an air stream during the NO2 adsorption process and prehumidization of the adsorbent with humid air had a beneficial effect on the obtained sorption capacities. In conclusion, the study demonstrated that pyrolysis of the extraction residues from the fungus Inonotus obliquus yields highly effective, environmentally friendly, and cost-efficient carbonaceous adsorbents for the removal of both gaseous and liquid pollutants.
A Coordination-Driven Self-Assembly and NIR Photothermal Conversion Study of Organometallic Handcuffs
Authors: Lu Xiaoyan , Huang Jing-Jing , Chen Tian , Zheng Jie , Liu Ming , Wang Xin-Yi , Li Yu-Xin , Niu Xinkai , Dang Li-Long
Published: September 2023

Due to their fascinating topological structures and application prospects, coordination supramolecular complexes have continuously been studied by scientists. However, the controlled construction and property study of organometallic handcuffs remains a significant and challenging research subject in the area of supramolecular chemistry. Hence, a series of tetranuclear organometallic and heterometallic handcuffs bearing different size and metal types were rationally designed and successfully synthesized by utilizing a quadridentate pyridyl ligand (tetra-(3-pyridylphenyl)ethylene) based on three Cp*Rh (Cp* = η5-C5Me5) fragments bearing specific longitudinal dimensions and conjugated planes. These results were determined with single-crystal X-ray diffraction analysis technology, ESI-MS NMR spectroscopy, etc. Importantly, the photoquenching effect of Cp* groups and the discrepancy of intermolecular π–π stacking interactions between building block and half-sandwich fragments promote markedly different photothermal conversion results. These results will further push the synthesis of topological structures and the development of photothermal conversion materials.
In Vitro and In Vivo Anti-Aging Effect of Coffee Berry Nanoliposomes
Authors: Saewan Nisakorn , Jimtaisong Ampa , Panyachariwat Nattakan , Chaiwut Phanuphong
Published: September 2023

Encapsulation of bioactive compounds in the liposome system provides several advantages, such as enhancing the stability and lowering the toxicity of active compounds. Coffee berry extract (CBE) has previously been established to have in vitro anti-aging properties and to retard the aging of human skin. The purposes of this study were to encapsulate CBE in nanoliposomes and to assess its stability and in vitro anti-aging potential in human dermal fibroblasts (HDF), as well as in healthy human skin. In the HDF model, anti-aging potential was determined by nitric oxide (NO) and collagenase inhibition assays and a superoxide dismutase (SOD) activity assay, whereas in healthy human skin (in vivo), the skin elasticity and brightness were examined. First, liposomal CBE (L-CBE) was created with a particle size of 117.33 ± 2.91 nm, a polydispersity index (PDI) of 0.36 ± 0.03, and a zeta potential of −56.13 ± 1.87 mV. The percentages of encapsulation efficacy (%EE) and loading efficacy (%LE) were 71.26 ± 3.12% and 2.18 ± 0.18%, respectively. After undergoing a 12-week stability test, the L-CBE retained more phenolic content than the free CBE when stored at 4 °C, room temperature, and 45 °C. Compared to free CBE, the L-CBE demonstrated a more consistent, elevated, and prolonged release of phenolics from the lipid system. In human dermal fibroblasts, L-CBE showed lower toxicity, and at its maximum nontoxic concentration (10 mg/mL), it exhibited slightly higher anti-aging effects than CBE, including NO inhibition, enhanced SOD activity, and anti-collagenase activities. In clinical trials (30 volunteer subjects), none of the participants’ skin was irritated when the L-CBE, the CBE, or base creams were applied. After 2 weeks of application, the L-CBE and CBE creams both demonstrated an improvement in skin elasticity and a reduction in melanin levels, and after 4 weeks, L-CBE cream showed a significantly greater improvement in skin elasticity and lightening. The results demonstrate that the encapsulation of the CBE in liposomal systems could increase its stability and skin penetration, reduce its toxicity, and maintain its anti-aging effect, which is powerful enough to be exploited in anti-aging and whitening agents for application in cosmetics and cosmeceuticals.