bluuebunny/crossref_metadata_2025 huggingface dataset:
Immunofluorescence Targeting PBP2a Protein: A New Potential Methicillin Resistance Screening Test
Authors: Silvestri Serenella , Rampacci Elisa , Stefanetti Valentina , Trotta Michele , Fani Caterina , Levorato Lucia , Brachelente Chiara , Passamonti Fabrizio
Published: December 2021

The indiscriminate use of first-line drugs contributed to the spread of resistant bacteria, a major concern for both human and veterinary medicine. Methicillin resistance is acquired through the mecA gene, which encodes for the PBP2a protein and lends the resistance to β-lactams. Verifying the correspondence between gene harboring and protein expression and accelerating methicillin resistance diagnosis is critical to improve the management of antimicrobial administration and to reduce the spread of drug resistances. We tested the applicability of immunofluorescence targeting PBP2a protein to identify a new potential methicillin resistance screening test, ancillary to conventional culture methods. We collected 26 clinical Staphylococcus pseudintermedius (SP) isolates: 25 from canine pyoderma and 1 from dermatitis in a dog owner. SP is one of the most important etiological agents in canine pyoderma and can harbor the mecA gene. We performed PCR for mecA gene detection, broth microdilution (BMD) for phenotypic methicillin resistance, and immunofluorescence targeting PBP2a protein. Compared to the PCR as the gold standard, immunofluorescence showed an apparent prevalence of 34.6% vs. a true prevalence of 53.8%, with 100% specificity, 64.3% sensitivity, and 80.8% diagnostic accuracy. PBP2a expression showed isolate-dependent variability: in some isolates, most of the bacterial cells showed an intense and clearly membranous pattern, while in others only a few of them could be detected. Performing the assay in duplicate improved the diagnostic accuracy. Since the mecA gene is shared among the members of the Staphylococcus genus, the test can be applied to identify methicillin resistance independently from the staphylococcal species, both in human and animal samples. Being a rapid and easy method and providing the unique possibility to study the expression of PBP2a by directly visualizing the morphology, it could represent a new interesting tool for both research and diagnostics. To accelerate methicillin resistance diagnosis, it would be worth further testing of its performance on cytological samples.
Dietary Hermetia illucens Larvae Replacement Alleviates Diarrhea and Improves Intestinal Barrier Function in Weaned Piglets Challenged With Enterotoxigenic Escherichia coli K88
Authors: Jin Xinxin , Yuan Boyu , Liu Mingming , Zhu Mingqiang , Zhang Xue , Xie Gaijie , Wu Wenxiang , Wang Zifan , Xu Haidong , Lv Yantao , Huang Yanhua , Wang Wei
Published: December 2021

A high-quality protein substitute, Hermetia illucens (black soldier fly) larvae powder, is rich in protein and often used in animal feed. This study aimed to investigate the feasibility and optimal ratio of replacing fish meal with H. illucens larvae in weaned piglets and to demonstrate the effects on piglets' growth performance, intestinal microflora and immune performance. Forty-eight female weaned piglets were randomly classified into three groups. Each group consisted of eight pens (replicates), with two piglets per pen. Three groups containing different proportions of H. illucens larvae (0, 4, and 8%) were referred to as C, HI4, and HI8. We first designed a 28-day feeding experiment to detect growth performance; after that, the piglets were induced with oral gavage of enterotoxigenic Escherichia coli K88 (ETEC K88) and recording diarrhea on day 29 of the experiment. Samples were taken on the 32nd day to detect the effect of H. illucens larvae on the immune performance of the weaned piglets. H. illucens larvae replacement did not cause any obvious change in the growth performance nether in HI4 nor in HI8 of weaned piglets with 28 d feeding stage. H. illucens larvae could improve the intestinal health of weaned piglets by increasing the content of Lactobacillus and reducing the content of Streptococcus. Compared with C+K88 group, the diarrhea rate was attenuated for the H. illucens supplemented group. The integrity of ileum villi in HI4+K88 and HI8+K88 groups was better than that in C+K88 group, and the villi in C+K88 group were severely damaged. The expression of IL-10, Occludin and Claudin-3 in the intestinal mucosa of the HI4+K88 group and HI8+K88 group were significantly increased (P < 0.05), and the expression of TNF-α was significantly decreased (P < 0.05) compared with the C+K88 group. The results of immunoblotting also validated that the same ETEC K88 treatment of weaned piglets enhanced the expression of tight junction protein in the intestinal mucosa of the H. illucens addition group. ETEC-induced diarrhea will be reduced by the diet of weaned piglets containing H. illucens larvae, ameliorating the immune performance of piglets. Our results indicates that the optimal dosage of H. illucens replacement in weaned piglets is 4%.
Potential Toxicity Evaluation of Protopine in Macleaya cordata (Willd.) R. Br.—A Bioactivity Guided Approach
Authors: Hu Wanjun , Yang Fan , Liu Weixue , Guo Liyang , Ai Liwen , Zhang Xiaomeng , Sheng Zunlai , Gao Chunbo
Published: December 2021

Macleaya cordata (Willd.) R. Br. (M. cordata) is a perennial herb known for its chemotherapeutic properties, strong feeding additive, and potential antidiarrheal drug. Despite its therapeutic potentials, its clinical applications are hindered by an apparent lack of toxicity data. In this study, the toxic ingredients of this plant were investigated using a bioactivity-guided approach. Two compounds, protopine and allocryptopine, were purified and elucidated by LC-MS, 1H-NMR, and 13C-NMR. Protopine, a primary component in M. cordata, had an LD50 of 313.10 mg/kg i.e., which was considered toxic. An autopsy was performed on protopine-administered mice, and the histopathology of the kidney, liver, brain, heart, lung, and spleen was determined. Autopsy findings included hemorrhage in the respiratory system, lung congestion, and hemorrhage and edema in the parenchymatous organs (heart, liver, kidney, and brain). Histopathology confirmed the pathological changes in the brain, liver, and kidney. Protopine is one of the principal bioactive constituents of many phytopreparations used in veterinary and human medicine, such as Sangrovit and Iberogast. Our findings indicated that phytopreparations containing protopine might pose a serious health threat to humans and animals.
The Buy-Online-Pick-Up-in-Store Retailing Model: Optimization Strategies for In-Store Picking and Packing
Authors: Pietri Nicola Ognibene , Chou Xiaochen , Loske Dominic , Klumpp Matthias , Montemanni Roberto
Published: December 2021

Online shopping is growing fast due to the increasingly widespread use of digital services. During the COVID-19 pandemic, the desire for contactless shopping has further changed consumer behavior and accelerated the acceptance of online grocery purchases. Consequently, traditional brick-and-mortar retailers are developing omnichannel solutions such as click-and-collect services to fulfill the increasing demand. In this work, we consider the Buy-Online-Pick-up-in-Store concept, in which online orders are collected by employees of the conventional stores. As labor is a major cost driver, we apply and discuss different optimizing strategies in the picking and packing process based on real-world data from a German retailer. With comparison of different methods, we estimate the improvements in efficiency in terms of time spent during the picking process. Additionally, the time spent on the packing process can be further decreased by applying a mathematical model that guides the employees on how to organize the articles in different shopping bags during the picking process. In general, we put forward effective strategies for the Buy-Online-Pick-up-in-Store paradigm that can be easily implemented by stores with different topologies.
Nutritional Quality and In Vitro Rumen Fermentation Characteristics of Silage Prepared with Lucerne, Sweet Maize Stalk, and Their Mixtures
Authors: Wang Musen , Zhang Fujin , Zhang Xinxin , Yun Ying , Wang Lei , Yu Zhu
Published: December 2021

The objective of this work was to evaluate the pH, chemical composition, minerals, vitamins, and in vitro rumen fermentation characteristics of silage prepared with lucerne, sweet maize stalk (MS), and their mixtures. Freshly chopped lucerne and MS were combined in ratios of 100:0 (M0, control), 80:20 (M20), 60:40 (M40), 40:60 (M60), 20:80 (M80), and 0:100 (M100) on a fresh matter basis. Each treatment was prepared in triplicate, and a total of eighteen silos were fermented for 65 days. After 65 days of fermentation, the pH values in M0, M20, M40, M60, M80, and M100 silages were 5.47, 4.84, 4.23, 4.13, 3.79, and 3.61, respectively. As the MS proportion in the mixtures increased, silage K, Ca, P, Na, Fe, and Cu concentrations linearly decreased (p < 0.001) and so did vitamins B5 and K1 and α-tocopherol. In vitro rumen dry matter and organic matter degradability, pH, ammonia, total volatile fatty acid, and gas production linearly decreased (p < 0.01), while neutral detergent fiber concentration linearly increased (p < 0.001), with increasing proportion of MS. The in vitro dry matter and organic matter degradability rapidly decreased when the MS percentage was ≥60%. In conclusion, the M40 silage is the most suitable for livestock utilization in local forage production considering the balance of silage pH, nutritional quality, and in vitro ruminal fermentation characteristics.
Effects of Chemical Fertilizer Combined with Organic Fertilizer Application on Soil Properties, Citrus Growth Physiology, and Yield
Authors: Wan Lian-Jie , Tian Yang , He Man , Zheng Yong-Qiang , Lyu Qiang , Xie Rang-Jin , Ma Yan-Yan , Deng Lie , Yi Shi-Lai
Published: December 2021

Chemical fertilizer has been excessively used for high yield of citrus around the world, especially in China; meanwhile, it deteriorates the citrus orchard soil environment. To resolve the conflict, the use of organic fertilizer provides a promising solution. However, the data about organic fertilizer used in citrus orchard is rarely available. Here, four treatments including CK (no fertilizer), CF (chemical fertilizer), OF + CF (chemical fertilizer reduction combined with organic fertilizer; application of N, P2O5, K2O fertilizer and organic fertilizer is 0.564, 0.236, 0.336 and 10 kg/plant), and BF + CF (chemical fertilizer reduction combined with bioorganic fertilizer; application of N, P2O5, K2O fertilizer and bioorganic fertilizer is 0.508, 0.320, 0.310 and 10 kg/plant) were performed in a ‘Ponkan’ (Citrus reticulata Blanco) orchard to evaluate the effect of organic fertilizer on citrus yield, growth, soil properties etc. when nutrients of fertilizer of each treatment were equal except CK. The data obtained in 2019 and 2020 showed that both OF + CF and BF + CF were beneficial to improve soil fertility (soil physicochemical and microbe properties) and citrus growth physiology (growth, nutrient and photosynthesis), alleviate NO3−-N leaching, and promote yields. Comprehensive evaluation indicated that BF + CF was more effective than OF + CF. Together, organic fertilizer has the potential to substitute partial chemical fertilizer with improvement in soil properties, growth physiology, and yield of citrus.
Stearoyl-CoA Desaturase Activity and Gene Expression in the Adipose Tissue of Buffalo Bulls Was Unaffected by Diets with Different Fat Content and Fatty Acid Profile
Authors: Iommelli Piera , Infascelli Federico , Musco Nadia , Grossi Micaela , Ferrara Maria , Sarubbi Fiorella , D’Aniello Biagio , Lombardi Pietro , Tudisco Raffaella
Published: December 2021

Research on diet effects on buffalo meat quality may be critical to assess its possible consumption benefits in human nutrition. This study investigated, in growing buffalo bulls, the effects of two diets differing in total fat content and fatty acid profile on the activity and gene expression of Stearoyl-CoA Desaturase (SCD) in the adipose tissue and on meat quality. Twenty buffalo bulls, 6 months old, were randomly assigned to the two dietary treatments until slaughtering (about 400 kg body weight). No significant difference between the groups was observed for chemical composition, fatty acid profile and CLAs content of Longissimus thoracis as well as for the SCD gene expression. Such results seem to be in contrast with similar studies performed on other ruminant species, but confirm that important differences occur between buffalo and bovine species, such as the lower content in fat of buffalo meat. Our results also confirm that specific studies should be performed on buffalo, also in terms of the metabolic pathways activated by different diets.
The Optimal Concentration of KH2PO4 Enhances Nutrient Uptake and Flower Production in Rose Plants via Enhanced Root Growth
Authors: Ma Qinghua , Wang Xinghong , Yuan Weijie , Tang Hongliang , Luan Mingbao
Published: December 2021

Monopotassium phosphate is a widely used phosphorus and potassium fertiliser for ornamental plants, but it is not known what concentration will result in optimal flower production, root growth and nutrient uptake of rose plants. We compared potted rose plants fertilised with six different concentrations (0.0 as a water-only control, 1.0, 2.0, 3.0, 4.0 and 5.0 g·L−1) of an aqueous monopotassium phosphate solution as a combination of foliar and soil applications over two consecutive flowering cycles. Rose growth, flower production and nutrient accumulation responded differently to fertilisation with different concentrations of monopotassium phosphate. During the first flowering cycle, shoot and root dry weight, leaf chlorophyll content, flower diameter, total root length and surface area, and total fine root length significantly increased in response to increased monopotassium phosphate concentrations from 0.0 to 3.0 g·L−1 but decreased in response to fertilisation with 4.0 or 5.0 g·L−1 monopotassium phosphate. Similar trends were observed in shoot dry weight, leaf chlorophyll content, flower diameter and number, phosphorus and potassium accumulation during the second flowering cycle. According to quadratic equations derived from both flowering cycles, the optimal concentration of monopotassium phosphate, based on flower diameter and dry weight, as well as total phosphorus and potassium accumulation, was 2.6–3.0 g·L−1. Furthermore, total root length was significantly correlated with flower diameter, flower dry weight, and total phosphorus and potassium accumulation (p < 0.05). These results indicated that fertilisation with optimal concentrations of monopotassium phosphate can increase rose growth, flower productivity and nutrient uptake through enhanced root growth.
Chemical Composition of Fir, Pine and Thyme Essential Oils and Their Effect on Onion (Allium cepa L.) Seed Quality
Authors: Dorna Hanna , Szopińska Dorota , Rosińska Agnieszka , Górski Romuald
Published: December 2021

Botrytis allii and B. cinerea are important seed-transmitted pathogens of onion. The aim of this study was to determine the chemical compositions of fir, pine and thyme essential oils and evaluate their effects on the germination, vigour and health of onion seeds. Gas Chromatography/Mass Spectroscopy was used for the analysis of the oils’ chemical compositions. Seed germination, vigour and health tests were performed on untreated seeds and on seeds treated with volatile compounds of the oils applied at a concentration of 0.2 µL cm−3 for 6, 12, 24, 48 and 72 h or at a concentration of 0.4 µL cm−3 for 3, 6 and 12 h. The main components of fir oil were bornyl acetate (55.5%) and α-pinene (15.6%), pine oil contained mostly α-pinene (35.5%) and β-pinene (18.6%), and thymol (34.2%) and p-cymene (26.2%) prevailed in thyme oil. Almost all treatments significantly reduced seed infestation with Alternaria alternata and Fusarium spp., and some effectively controlled Botrytis spp., however, seed health improvement was usually associated with deterioration of seed germination and vigour. Only treatment with fir oil at a concentration of 0.2 µL cm−3 for 6 h effectively controlled seed-borne pathogens and positively affected seed germination. Considering the beneficial effect of the oils on seed health, further studies are necessary in order to establish the optimal conditions for onion seed treatment.
Seeding Rate Effects on Forage Mass and Vegetation Dynamics of Cool-Season Grass Sod Interseeded with Sorghum-Sudangrass
Authors: Guretzky John A. , Redfearn Daren D.
Published: December 2021

Interseeding annual warm-season grasses into perennial cool-season grasses has the potential to increase summer forage mass and nutritive value. Knowledge of how seeding rate affects annual warm-season grass establishment, forage mass, and vegetation dynamics remains limited. From 2016–2017, we conducted a field experiment evaluating the effects of seeding rates on sorghum-sudangrass (Sorghum bicolor × S. bicolor var. sudanense) density and forage mass and on the frequency of occurrence of plant species in cool-season grass sod in Lincoln, NE. The experiment had a completely randomized design consisting of six replicates of four seeding rates [0, 14, 28, and 35 kg pure live seed (PLS) ha−1] in sod mowed at a 2.5-cm height and one unseeded, non-mowed control treatment. Sorghum-sudangrass establishment increased with seeding rate from an average of 20 to 45 plants m−2 as the seeding rate increased from 14 to 35 kg PLS ha−1. Forage mass depended on a seeding rate × harvest interaction, showing positive linear and cubic responses to seeding rate in consecutive harvests at 45 and 90 d after interseeding. To increase forage mass in perennial cool-season grass sod, producers should interseed sorghum-sudangrass with at least 28 kg PLS ha−1. One-time seedings into cool-season, perennial grass sod have no residual effects on subsequent forage mass and vegetation dynamics.
Characterization of Molecular Properties and Expression of Gene GmPLMT and Its Effects on the Production of Lipid Metabolites in Soybean and Arabidopsis thaliana
Authors: Chen Zhanyu , Wang Yushuang , Chen Yanbo , Yang Xiaoqin , Wang Shuang , Yu Tingting , Zhou Ying , Cui Xiyan
Published: December 2021

Phospholipid N-methyltransferase (PLMT) plays an important role in the synthesis of phosphatidylcholine (PtdCho). The aim of this study was to characterize the molecular properties of GmPLMT and the expression of soybean GmPLMT and its effects on the production of lipid metabolites. Results showed that GmPLMT composed of mainly α-helix was a hydrophobic and transmembrane protein. In soybean leaves, GmPLMT was highly expressed during seedling and flowering stages. In transgenic Arabidopsis thaliana, the highest and lowest expression levels of GmPLMT were detected at flowering and maturity stages, respectively. The total phospholipid contents in soybean grains were decreased from 7.2% (35 days after flowering) to 4.8% (55 days after flowering) and then increased to 7.0% (75 days after flowering). The contents of PtdCho showed a similar pattern to that of total phospholipids. In transgenic A. thaliana seeds, the contents of total phospholipids and PtdCho were significantly increased. Significantly positive correlations were revealed between expression of GmPLMT and contents of both PtdCho and crude fats, and between the contents of PtdCho and both linoleic acid and linolenic acid, suggesting that increased expression of GmPLMT improved the production of lipid metabolites. This study provided solid experimental evidence for further improvement of soybean quality based on GmPLMT in the molecular breeding of soybeans.
The Analysis of Chitosan-Coated Nanovesicles Containing Erythromycin—Characterization and Biocompatibility in Mice
Authors: Hilițanu Loredana Nicoleta , Mititelu-Tarțău Liliana , Popa Grațiela Eliza , Buca Beatrice Rozalina , Pavel Liliana Lăcrămioara , Pelin Ana-Maria , Meca Andreea-Daniela , Bogdan Maria , Pricop Daniela Angelica
Published: December 2021

Nanoantibiotics have proved improved pharmacokinetic characteristics and antimicrobial features. Recent studies have shown non-toxicity, non-immunogenicity, antioxidant, anti-hyperlipidemic, and hepatocyte protective actions, among other advantages of chitosan-based nanoparticles. The purpose of our study was the structural analysis of novel chitosan-coated vesicles entrapping erythromycin (ERT) and the assessment of their biocompatibility in mice. According to the group in which they were randomly assigned, the mice were treated orally with one of the following: distilled water; chitosan; ERT; chitosan vesicles containing ERT. Original nanosystems entrapping ERT in liposomes stabilized with chitosan were designed. Their oral administration did not produce sizeable modifications in the percentages of the leukocyte formula elements, of some blood constants useful for evaluating the hepatic and renal function, respectively, and of some markers of oxidative stress and immune system activity, which suggests a good biocompatibility in mice. The histological examination did not reveal significant alterations of liver and kidney architecture in mice treated with chitosan liposomes entrapping ERT. The results indicate the designed liposomes are a promising approach to overcome disadvantages of conventional ERT treatments and to amplify their benefits and can be further studied as carrier systems.
Structural Analysis, Phase Stability, Electronic Band Structures, and Electric Transport Types of (Bi2)m(Bi2Te3)n by Density Functional Theory Calculations
Authors: Park Sungjin , Ryu Byungki , Park SuDong
Published: December 2021

Thermoelectric power generation is a promising candidate for automobile energy harvesting technologies because it is eco-friendly and durable owing to direct power conversion from automobile waste heat. Because Bi−Te systems are well-known thermoelectric materials, research on (Bi2)m(Bi2Te3)n homologous series can aid the development of efficient thermoelectric materials. However, to the best of our knowledge, (Bi2)m(Bi2Te3)n has been studied through experimental synthesis and measurements only. Therefore, we performed density functional theory calculations of nine members of (Bi2)m(Bi2Te3)n to investigate their structure, phase stability, and electronic band structures. From our calculations, although the total energies of all nine phases are slightly higher than their convex hulls, they can be metastable owing to their very small energy differences. The electric transport types of (Bi2)m(Bi2Te3)n do not change regardless of the exchange–correlation functionals, which cause tiny changes in the atomic structures, phase stabilities, and band structures. Additionally, only two phases (Bi8Te9, BiTe) became semimetallic or semiconducting depending on whether spin–orbit interactions were included in our calculations, and the electric transport types of the other phases were unchanged. As a result, it is expected that Bi2Te3, Bi8Te9, and BiTe are candidates for thermoelectric materials for automobile energy harvesting technologies because they are semiconducting.
Behavior of Non-Shear-Strengthened UHPC Beams under Flexural Loading: Influence of Reinforcement Percentage
Authors: Khan Mohammad Iqbal , Fares Galal , Abbas Yassir M. , Alqahtani Fahad K.
Published: December 2021

In the present work, the structural responses of 12 UHPC beams to four-point loading conditions were experimentally and analytically studied. The inclusion of a fibrous system in the UHPC material increased its compressive and flexural strengths by 31.5% and 237.8%, respectively. Improved safety could be obtained by optimizing the tensile reinforcement ratio (ρ) for a UHPC beam. The slope of the moment–curvature before and after steel yielding was almost typical for all beams due to the inclusion of a hybrid fibrous system in the UHPC. Moreover, we concluded that as ρ increases, the deflection ductility exponentially increases. The cracking response of the UHPC beams demonstrated that increasing ρ notably decreases the crack opening width of the UHPC beams at the same service loading. The cracking pattern the beams showed that increasing the bar reinforcement percentages notably enhanced their initial stiffness and deformability. Moreover, the flexural cracks were the main cause of failure for all beams; however, flexure shear cracks were observed in moderately reinforced beams. The prediction efficiency of the proposed analytical model was established by performing a comparative study on the experimental and analytical ultimate moment capacity of the UHPC beams. For all beams, the percentage of the mean calculated moment capacity to the experimentally observed capacity approached 100%.
Consistency Analysis and Accuracy Assessment of Eight Global Forest Datasets over Myanmar
Authors: Xing Huaqiao , Niu Jingge , Liu Chang , Chen Bingyao , Yang Shiyong , Hou Dongyang , Zhu Linye , Hao Wenjun , Li Cansong
Published: December 2021

Accurate and up-to-date forest monitoring plays a significant role in the country’s society and economy. Many open-access global forest datasets can be used to analyze the forest profile of countries around the world. However, discrepancies exist among these forest datasets due to their specific classification systems, methodologies, and remote sensing data sources, which makes end-users difficult to select an appropriate dataset in different regions. This study aims to explore the accuracy, consistency, and discrepancies of eight widely-used forest datasets in Myanmar, including Hansen2010, CCI-LC2015, FROM-GLC2015/2017, FROM-GLC10, GLC-FCS2015/2020, and GlobeLand30-2020. Firstly, accuracy assessment is conducted by using 934 forest and non-forest samples with four different years. Then, spatial consistency of these eight datasets is compared in area and spatial distribution. Finally, the factors influencing the spatial consistency are analyzed from the aspects of terrain and climate. The results indicate that in Myanmar the forest area derived from GlobeLand30 has the best accuracy, followed by FROM-GLC10 and FROM-GLC2017. The eight datasets differ in spatial detail, with the mountains of northern Myanmar having the highest consistency and the seaward areas of southwestern Myanmar having the highest inconsistency, such as Rakhine and the Ayeyarwady. In addition, it is found that the spatial consistency of the eight datasets is closely related to the terrain and climate. The highest consistency among the eight datasets is found in the range of 1000–3500 m above sea level and 26°–35° slope. In the subtropical highland climate (Cwb) zone, the percentage of complete consistency among the eight datasets is as high as 60.62%, which is the highest consistency among the six climatic zones in Myanmar. Therefore, forest mapping in Myanmar should devote more effort to low topography, seaward areas such as border states like Rakhine, Irrawaddy, Yangon, and Mon. This is because these areas have complex and diverse landscape types and are prone to confusion between forest types (e.g., grassland, shrub, and cropland). The approach can also be applied to other countries, which will help scholars to select the most suitable forest datasets in different regions for analysis, thus providing recommendations for relevant forest policies and planning in different countries.
Modeling and Control of a Microgrid Connected to the INTEC University Campus
Authors: Aybar-Mejía Miguel , León-Viltre Lesyani , Santos Félix , Neves Francisco , Gómez Víctor Alonso , Mariano-Hernández Deyslen
Published: December 2021

A smart microgrid is a bidirectional electricity generation system—a type of system that is becoming more prevalent in energy production at the distribution level. Usually, these systems have intermittent renewable energy sources, e.g., solar and wind energy. These low voltage networks contribute to decongestion through the efficient use of resources within the microgrid. In this investigation, an energy management strategy and a control scheme for DG units are proposed for DC/AC microgrids. The objective is to implement these strategies in an experimental microgrid that will be developed on the INTEC university campus. After presenting the microgrid topology, the modeling and control of each subsystem and their respective converters are described. All possible operation scenarios, such as islanded or interconnected microgrids, different generation-load possibilities, and state-of-charge conditions of the battery, are verified, and a seamless transition between different operation modes is ensured. The simulation results in Matlab Simulink show how the proposed control system allows transitions between the different scenarios without severe transients in the power transfer between the microgrid and the low voltage network elements.
Relationship between the Somatosensory Cortex Morphology, Cutaneous Allodynia, and Clinical Features of Patients with Migraine
Authors: Carvalho Gabriela Ferreira , Maciel Nicoly Machado , Arruda Eduardo , Pinheiro Carina Ferreira , Oliveira Natália , Correia Rodolfo Dias Chiari , Dach Fabiola , Santos Antonio Carlos dos , Bevilaqua-Grossi Debora
Published: December 2021

Recent studies have demonstrated the presence of brain alterations in patients with migraine. Functional and vascular changes in the brain are related to the presence and severity of cutaneous allodynia. However, the association between brain structural changes and cutaneous allodynia has not been yet investigated in patients with migraine. Thus, the purpose of this study was to evaluate the correlation between the severity of cutaneous allodynia, migraine features, and the thickness and volume of the somatosensory cortex. Forty-five patients with migraine, with and without aura and chronic migraine, were included. Volunteers filled out the Allodynia Symptom Questionnaire (ASC-12/Brazil) and were evaluated via magnetic resonance imaging (MRI). The images were inspected by a blinded neuroradiologist and analyzed with Freesurfer software. Correlation tests and a linear regression model were used to evaluate the relationship among the outcomes. The somatosensory cortex thickness and volume were not different among migraine subgroups (p > 0.05). There was no significant correlation between the somatosensory thickness and volume with the ASC-12/Brazil, migraine frequency, intensity, migraine onset or aura frequency. The ASC-12/Brazil score variability cannot be predicted by the somatosensory cortex thickness or volume. The results show that the somatosensory cortex morphology is neither associated with cutaneous allodynia nor with migraine features among migraineurs.
Application of the Extended Reality Technology for Teaching New Languages: A Systematic Review
Authors: Tegoan Noura , Wibowo Santoso , Grandhi Srimannarayana
Published: December 2021

Much attention has been given to the use of extended reality (XR) technology in educational institutions due to its flexibility, effectiveness, and attractiveness. However, there is a limited study of the application of XR technology for teaching and learning languages in schools. Thus, this paper presents a systematic review to identify the potential benefits and challenges of using XR technology for teaching new languages. This review provides a basis for adopting XR technology for teaching languages in schools. This research also provides recommendations to successfully implement the XR technology and ways to improve motivation, engagement, and enhanced accessibility of learning and teaching resources on both students and teachers. To fulfil the aims of this research, previous studies from 2011 to 2021 are collected from various academic databases. This study finds that there is still a need to develop appropriate strategies for the development and implementation of XR technology for teaching new languages to school students.
Recent Developments on Hydrogen Production Technologies: State-of-the-Art Review with a Focus on Green-Electrolysis
Authors: Vidas Leonardo , Castro Rui
Published: December 2021

Growing human activity has led to a critical rise in global energy consumption; since the current main sources of energy production are still fossil fuels, this is an industry linked to the generation of harmful byproducts that contribute to environmental deterioration and climate change. One pivotal element with the potential to take over fossil fuels as a global energy vector is renewable hydrogen; but, for this to happen, reliable solutions must be developed for its carbon-free production. The objective of this study was to perform a comprehensive review on several hydrogen production technologies, mainly focusing on water splitting by green-electrolysis, integrated on hydrogen’s value chain. The review further deepened into three leading electrolysis methods, depending on the type of electrolyzer used—alkaline, proton-exchange membrane, and solid oxide—assessing their characteristics, advantages, and disadvantages. Based on the conclusions of this study, further developments in applications like the efficient production of renewable hydrogen will require the consideration of other types of electrolysis (like microbial cells), other sets of materials such as in anion-exchange membrane water electrolysis, and even the use of artificial intelligence and neural networks to help design, plan, and control the operation of these new types of systems.
Collagen Nanoparticles in Drug Delivery Systems and Tissue Engineering
Authors: Arun Ashni , Malrautu Pratyusha , Laha Anindita , Luo Hongrong , Ramakrishna Seeram
Published: December 2021

The versatile natural polymer, collagen, has gained vast attention in biomedicine. Due to its biocompatibility, biodegradability, weak antigenicity, biomimetics and well-known safety profile, it is widely used as a drug, protein and gene carrier, and as a scaffold matrix in tissue engineering. Nanoparticles develop favorable chemical and physical properties such as increased drug half-life, improved hydrophobic drug solubility and controlled and targeted drug release. Their reduced toxicity, controllable characteristics of scaffolds and stimuli-responsive behavior make them suitable in regenerative medicine and tissue engineering. Collagen associates and absorbs nanoparticles leading to significant impacts on their biological functioning in any biofluid. This review will discuss collagen nanoparticle preparation methods and their applications and developments in drug delivery systems and tissue engineering.
Identification of Fractional Damping Parameters in Structural Dynamics Using Polynomial Chaos Expansion
Authors: Prem Marcel S. , Klanner Michael , Ellermann Katrin
Published: December 2021

In order to analyze the dynamics of a structural problem accurately, a precise model of the structure, including an appropriate material description, is required. An important step within the modeling process is the correct determination of the model input parameters, e.g., loading conditions or material parameters. An accurate description of the damping characteristics is a complicated task, since many different effects have to be considered. An efficient approach to model the material damping is the introduction of fractional derivatives in the constitutive relations of the material, since only a small number of parameters is required to represent the real damping behavior. In this paper, a novel method to determine the damping parameters of viscoelastic materials described by the so-called fractional Zener material model is proposed. The damping parameters are estimated by matching the Frequency Response Functions (FRF) of a virtual model, describing a beam-like structure, with experimental vibration data. Since this process is generally time-consuming, a surrogate modeling technique, named Polynomial Chaos Expansion (PCE), is combined with a semi-analytical computational technique, called the Numerical Assembly Technique (NAT), to reduce the computational cost. The presented approach is applied to an artificial material with well defined parameters to show the accuracy and efficiency of the method. Additionally, vibration measurements are used to estimate the damping parameters of an aluminium rotor with low material damping, which can also be described by the fractional damping model.
Giving Absurdity Form: The Place of Contemporary Art in the Environmental Crisis
Authors: Douglas Anne Elizabeth
Published: December 2021

Absurdity in art creates bizarre juxtapositions that expose, and question conflicted, even dangerous, aspects of life which have become normalized. Absurd art appears in troubled times, subverting moments of extreme contradiction in which it appears impossible to think differently. For example, Dada (1917–1923) used nonsense to reflect the nonsensical brutality of WW1. The power to unsettle in this form of art rests in disrupting the world of the viewer and positioning them as interlocutors in a new framing. Absurdity in art reveals the absurdity that is inherent in life and its institutions, breaking the illusion of control. It can help us to comprehend the ‘incomprehensible’ in other species and spheres of life. In the challenge of anthropogenic climate change, how might the absurd capture the strangeness of current times in which a gap is widening between the earth we live ‘in’ and the earth we live ‘from’? This article explores qualities of the absurd in art as a possible way in which to grasp and reimagine ourselves beyond the anthropocentric, focusing on the work of the artists John Newling (b. 1952, UK) and Helen Mayer (1927–2018, US) and Newton Harrison (b. 1932, US), known as ‘The Harrisons’.
Oversized Electrical Appliance Impacts on Condominium Energy Efficiency and Cost-Effectiveness Management: Experts’ Perspectives
Authors: Buranaaudsawakul Techatat , Wisaeng Kittipol
Published: December 2021

A direct use approach incorporating a cost approach assumed that replacing oversized electrical appliances with those better fit to actual energy consumption can reduce energy consumption, optimizing capacities of the new appliances to the maximum while reducing electricity costs. This study aimed to verify the assumption that the size of appliances has impacts on energy consumption and cost effectiveness. A mixed-method approach included these instruments for data elicitations (i.e., a questionnaire, data records of 485 transformers, two assessments of condominium technical caretakers, and two in-depth interviews of electrical engineering experts). The findings revealed that most condominiums installed electric appliances that are too large for their actual energy usage, which lies between 5.4% and 7.1% of the capacity. This study therefore proposed a total cost reduction of 54% by downsizing these appliances (i.e., MV Switchgear 2 sets, dry type transformer 2 sets 80,000, LV Cable 10 m. (XLPE), main distribution board, Busduct (MDB-DB), generator (20% of Tr.), and generator installation). Even though this analysis is limited to Bangkok, Thailand, this case may contribute decision-making on electrical appliance selection at early stage of investment or to downsize the currently installed appliances for the more energy efficient and cost-effective management of condominiums around the world.
Hydrogen Bond Arrangement Is Shown to Differ in Coexisting Phases of Aqueous Two-Phase Systems
Authors: Madeira Pedro P. , Titus Amber R. , Ferreira Luisa A. , Belgovskiy Alexander I. , Mann Elizabeth K. , Mann Jay Adin , Meyer William V. , Smart Anthony E. , Uversky Vladimir N. , Zaslavsky Boris Y.
Published: December 2021

Analysis by attenuated total reflection–Fourier transform infrared spectroscopy shows that each coexisting phase in aqueous two-phase systems has a different arrangement of hydrogen bonds. Specific arrangements vary for systems formed by different solutes. The hydrogen bond arrangement is shown to correlate with differences in hydrophobic and electrostatic properties of the different phases of five specific systems, four formed by two polymers and one by a single polymer and salt. The results presented here suggest that the arrangement of hydrogen bonds may be an important factor in phase separation.
Enhancing the Potential of Immunotherapy in Paediatric Sarcomas: Breaking the Immunosuppressive Barrier with Receptor Tyrosine Kinase Inhibitors
Authors: Fleuren Emmy D. G. , Terry Rachael L. , Meyran Deborah , Omer Natacha , Trapani Joseph A. , Haber Michelle , Neeson Paul J. , Ekert Paul G.
Published: December 2021

Despite aggressive surgery, chemotherapy, and radiotherapy, survival of children and adolescents and young adults (AYAs) with sarcoma has not improved significantly in the past four decades. Immune checkpoint inhibitors (ICIs) are an exciting type of immunotherapy that offer new opportunities for the treatment of paediatric and AYA sarcomas. However, to date, most children do not derive a benefit from this type of treatment as a monotherapy. The immunosuppressive tumour microenvironment is a major barrier limiting their efficacy. Combinations of ICIs, such as anti-PD-1 therapy, with targeted molecular therapies that have immunomodulatory properties may be the key to breaking through immunosuppressive barriers and improving patient outcomes. Preclinical studies have indicated that several receptor tyrosine kinase inhibitors (RTKi) can alter the tumour microenvironment and boost the efficacy of anti-PD-1 therapy. A number of these combinations have entered phase-1/2 clinical trials, mostly in adults, and in most instances have shown efficacy with manageable side-effects. In this review, we discuss the status of ICI therapy in paediatric and AYA sarcomas and the rationale for co-treatment with RTKis. We highlight new opportunities for the integration of ICI therapy with RTK inhibitors, to improve outcomes for children with sarcoma.
Genomic and Personalized Medicine Approaches for Substance Use Disorders (SUDs) Looking at Genome-Wide Association Studies
Authors: Cozzoli Danilo , Daponte Alessia , De Fazio Salvatore , Ariano Vincenza , Quaranta Maria Rita , Leone Vincenzo , Ostuni Angelo , Casanova Margherita , Catacchio Claudia Rita , Ventura Mario , Montinaro Francesco
Published: December 2021

Drug addiction, or substance use disorder (SUD), is a chronic, relapsing disorder in which compulsive drug-seeking and drug-taking behaviour persist despite serious negative consequences. Drug abuse represents a problem that deserves great attention from a social point of view, and focuses on the importance of genetic studies to help in understanding the genetic basis of addiction and its medical treatment. Despite the complexity of drug addiction disorders, and the high number of environmental variables playing a role in the onset, recurrence, and duration of the symptoms, several studies have highlighted the non-negligible role of genetics, as demonstrated by heritability and genome-wide association studies. A correlation between the relative risk of addiction to specific substances and heritability has been recently observed, suggesting that neurobiological mechanisms may be, at least in part, inherited. All these observations point towards a scenario where the core neurobiological factors of addiction, involving the reward system, impulsivity, compulsivity, stress, and anxiety response, are transmitted, and therefore, genes and mutations underlying their variation might be detected. In the last few years, the development of new and more efficient sequencing technologies has paved the way for large-scale studies in searching for genetic and epigenetic factors affecting drug addiction disorders and their treatments. These studies have been crucial to pinpoint single nucleotide polymorphisms (SNPs) in genes that affect the reaction to medical treatments. This is critically important to identify pharmacogenomic approaches for substance use disorder, such as OPRM1 SNPs and methadone required doses for maintenance treatment (MMT). Nevertheless, despite the promising results obtained by genome-wide association and pharmacogenomic studies, specific studies related to population genetics diversity are lacking, undermining the overall applicability of the preliminary findings, and thus potentially affecting the portability and the accuracy of the genetic studies. In this review, focusing on cannabis, cocaine and heroin use, we report the state-of-the-art genomics and pharmacogenomics of SUDs, and the possible future perspectives related to medical treatment response in people that ask for assistance in solving drug-related problems.
Adherence to Antiplatelet Medications among Persistent and Non-Persistent Older Patients with Peripheral Arterial Disease
Authors: Wawruch Martin , Murin Jan , Tesar Tomas , Paduchova Martina , Petrova Miriam , Celovska Denisa , Matalova Petra , Havelkova Beata , Trnka Michal , Aarnio Emma
Published: December 2021

Secondary prevention of peripheral arterial disease (PAD) includes administration of antiplatelet agents, and adherence to medication is a requirement for an effective treatment. The aim of this study was to analyse adherence measured using the proportion of days covered (PDC) index separately in persistent and non-persistent patients, and to identify patient- and medication-related characteristics associated with non-adherence in these patient groups. The study cohort of 9178 patients aged ≥ 65 years in whom PAD was diagnosed in 1/–12/2012 included 6146 persistent and 3032 non-persistent patients. Non-adherence was identified as PDC < 80%. Characteristics associated with non-adherence were determined using the binary logistic regression model. In the group of persistent patients, 15.3% of subjects were identified as non-adherent, while among non-persistent patients, 26.9% of subjects were non-adherent to antiplatelet medication. Administration of dual antiplatelet therapy (aspirin and clopidogrel) and a general practitioner as index prescriber were associated with adherence in both patient groups. Our study revealed a relatively high proportion of adherent patients not only in the group of persistent patients but also in the group of non-persistent patients before discontinuation. These results indicate that most non-persistent PAD patients discontinue antiplatelet treatment rapidly after a certain period of adherence.
Ultrasound Technologies and the Diagnosis of Giant Cell Arteritis
Authors: Jianu Dragoș Cătălin , Jianu Silviana Nina , Dan Traian Flavius , Munteanu Georgiana , Bîrdac Claudiu Dumitru , Motoc Andrei Gheorghe Marius , Docu Axelerad Any , Petrica Ligia , Gogu Anca Elena
Published: December 2021

Giant cell arteritis (GCA) is a primary autoimmune vasculitis that specifically affects medium-sized extracranial arteries, like superficial temporal arteries (TAs). The most important data to be considered for the ultrasound (US) diagnosis of temporal arteritis are stenosis, acute occlusions and “dark halo” sign, which represent the edema of the vascular wall. The vessel wall thickening of large vessels in GCA can be recognized by the US, which has high sensitivity and is facile to use. Ocular complications of GCA are common and consist especially of anterior arterial ischemic optic neuropathies or central retinal artery occlusion with sudden, painless, and sharp loss of vision in the affected eye. Color Doppler imaging of the orbital vessels (showing low-end diastolic velocities and a high resistance index) is essential to quickly differentiate the mechanism of ocular involvement (arteritic versus non-arteritic), since the characteristics of TAs on US do not correspond with ocular involvement on GCA. GCA should be cured immediately with systemic corticosteroids to avoid further visual loss of the eyes.