bluuebunny/crossref_metadata_2025 huggingface dataset:
Population Size, Sex and Purifying Selection: Comparative Genomics of Two Sister Taxa of the Wild Yeast Saccharomyces paradoxus
Authors: Koufopanou Vassiliki , Lomas Susan , Pronina Olga , Almeida Pedro , Sampaio Jose Paulo , Mousseau Timothy , Liti Gianni , Burt Austin
Published: July 2020

This study uses population genomic data to estimate demographic and selection parameters in two sister lineages of the wild yeast Saccharomyces paradoxus and compare their evolution. We first estimate nucleotide and recombinational diversities in each of the two lineages to infer their population size and frequency of sex and then analyze the rate of mutation accumulation since divergence from their inferred common ancestor to estimate the generation time and efficacy of selection. We find that one of the lineages has significantly higher silent nucleotide diversity and lower linkage disequilibrium, indicating a larger population with more frequent sexual generations. The same lineage also shows shorter generation time and higher efficacy of purifying selection, the latter consistent with the finding of larger population size and more frequent sex. Similar analyses are also performed on the ancestries of individual strains within lineages and we find significant differences between strains implying variation in rates of mitotic cell divisions. Our sample includes some strains originating in the Chernobyl nuclear-accident exclusion zone, which has been subjected to high levels of radiation for nearly 30 years now. We find no evidence, however, for increased rates of mutation. Finally, there is a positive correlation between rates of mutation accumulation and length of growing period, as measured by latitude of the place of origin of strains. Our study illustrates the power of genomic analyses in estimating population and life history parameters and testing predictions based on population genetic theory.
Metabolic Signatures of Life Span Regulated by Mating, Sex Peptide, and Mifepristone/RU486 in FemaleDrosophila melanogaster
Authors: Landis Gary N , Doherty Devon V , Yen Chia-An , Wang Lu , Fan Yang , Wang Ina , Vroegop Jonah , Wang Tianyi , Wu Jimmy , Patel Palak , Lee Shinwoo , Abdelmesieh Mina , Shen Jie , Promislow Daniel E L , Curran Sean P , Tower John
Published: July 2020

Mating and transfer of male sex peptide (SP), or transgenic expression of SP, causes inflammation and decreased life span in female Drosophila. Mifepristone rescues these effects, yielding dramatic increases in life span. Here targeted metabolomics data were integrated with further analysis of extant transcriptomic data. Each of 7 genes positively correlated with life span were expressed in the brain or eye and involved regulation of gene expression and signaling. Genes negatively correlated with life span were preferentially expressed in midgut and involved protein degradation, amino acid metabolism, and immune response. Across all conditions, life span was positively correlated with muscle breakdown product 1/3-methylhistidine and purine breakdown product urate, and negatively correlated with tryptophan breakdown product kynurenic acid, suggesting a SP-induced shift from somatic maintenance/turnover pathways to the costly production of energy and lipids from dietary amino acids. Some limited overlap was observed between genes regulated by mifepristone and genes known to be regulated by ecdysone; however, mifepristone was unable to compete with ecdysone for activation of an ecdysone-responsive transgenic reporter. In contrast, genes regulated by mifepristone were highly enriched for genes regulated by juvenile hormone (JH), and mifepristone rescued the negative effect of JH analog methoprene on life span in adult virgin females. The data indicate that mifepristone increases life span and decreases inflammation in mated females by antagonizing JH signaling downstream of male SP. Finally, mifepristone increased life span of mated, but not unmated, Caenorhabditis elegans, in 2 of 3 trials, suggesting possible evolutionary conservation of mifepristone mechanisms.
Personality Development Beyond the Mean: Do Life Events Shape Personality Variability, Structure, and Ipsative Continuity?
Authors: Jackson Joshua J , Beck Emorie D
Published: July 2020

Objectives

Life experiences are thought to prompt changes in personality. However, existing studies find few replicable mean-level changes in personality following life events. The focus on mean-level change may obscure other types of personality change that are not routinely studied in the context of life events. These are variability in response, structural, and ipsative change.

Methods

The current proposal examines whether major life events (e.g., divorce and job loss) affect these 3 understudied types of personality trait change using 3 waves of Big Five trait data in a large-scale, representative longitudinal study (German Socioeconomic Panel Study, N = 16,368). Structural equation models compare those who had an event to their prior self and a control group who did not experience the event.

Results

Life events were found to have mostly null or small effects on variability in response, structural, and ipsative change. Across 2 types of tests for variability in response, few replications occurred. The only consistent effect across 3 types of change was for mental health events, which served to increase variance in all Big Five traits and increase consistency in ipsative profiles.

Discussion

Life events tend not to affect these novel metrics of personality trait change. The one exception of mental health events is consistent with previous literature on mean-level change. Overall, life events do not appear to by major catalysts of personality change, regardless of how change is defined.
Imputing missing RNA-sequencing data from DNA methylation by using a transfer learning–based neural network
Authors: Zhou Xiang , Chai Hua , Zhao Huiying , Luo Ching-Hsing , Yang Yuedong
Published: July 2020

Background

Gene expression plays a key intermediate role in linking molecular features at the DNA level and phenotype. However, owing to various limitations in experiments, the RNA-seq data are missing in many samples while there exist high-quality of DNA methylation data. Because DNA methylation is an important epigenetic modification to regulate gene expression, it can be used to predict RNA-seq data. For this purpose, many methods have been developed. A common limitation of these methods is that they mainly focus on a single cancer dataset and do not fully utilize information from large pan-cancer datasets.

Results

Here, we have developed a novel method to impute missing gene expression data from DNA methylation data through a transfer learning–based neural network, namely, TDimpute. In the method, the pan-cancer dataset from The Cancer Genome Atlas (TCGA) was utilized for training a general model, which was then fine-tuned on the specific cancer dataset. By testing on 16 cancer datasets, we found that our method significantly outperforms other state-of-the-art methods in imputation accuracy with a 7–11% improvement under different missing rates. The imputed gene expression was further proved to be useful for downstream analyses, including the identification of both methylation–driving and prognosis-related genes, clustering analysis, and survival analysis on the TCGA dataset. More importantly, our method was indicated to be useful for general purposes by an independent test on the Wilms tumor dataset from the Therapeutically Applicable Research to Generate Effective Treatments (TARGET) project.

Conclusions

TDimpute is an effective method for RNA-seq imputation with limited training samples.
The media and health education: Did Nigerian media provide sufficient warning messages on coronavirus disease?
Authors: Gever Verlumun Celestine , Ezeah Gregory
Published: July 2020

Previous studies on media coverage of health issues hardly recognize the role of time in moderating media contents. Instead, scholars most often examine how news media report health issues. In this study, we recognized the role of time by taking into account how media report differs based on when a global outbreak is confirmed in a country and when it is not. We focused on coronavirus disease 2019 (COVID-19) and examined six media—two TV stations, two newspapers and two radio stations. We content-analysed 537 stories and found that there were few stories about the virus before it was confirmed in Nigeria. But as soon as Nigeria recorded a confirmed case, the number of stories tripled. We also noticed that story format and recommendation on health behaviour were also closely linked to the COVID-19 status of Nigeria. However, we did not find an association between Nigeria’s COVID-19 status and policy recommendation among the media studied. Therefore, this study concludes that Nigerian media did not provide sufficient health warning messages on COVID-19 before its spread to the country.
Digital Distraction or Stimulated Self-Disclosure: Preadolescents’ Mobile Device Use in the Family Context
Authors: Meeus Anneleen , Eggermont Steven , Beullens Kathleen
Published: July 2020

This study explored the social repercussions of preadolescents’ mobile device use within the family context by testing two opposing predictions regarding the impact of technology on the quality of interpersonal relationships. Specifically, we examined whether smartphone and tablet use was positively related to preadolescents’ self-disclosure to their parents and/or displaced family time, which we hypothesized to be related to children’s satisfaction with family life. Results of a cross-sectional survey (n = 698, 49.6% girls, Mage= 10.9, SD = 0.69) provided support for both hypotheses, thus corroborating the presence of at least two diverging pathways that underlie this relationship. Although mobile device use seemed to foster a context that supported children’s self-disclosure to their parents, results also indicated a negative and indirect association through children’s perceived family time. This negative pathway, however, did not hold when social use among children and parents (e.g., playing games together online) was considered separately.
CD33 and SIGLECL1 Immunoglobulin Superfamily Involved in Dementia
Authors: Rendina Antonella , Drongitis Denise , Donizetti Aldo , Fucci Laura , Milan Graziella , Tripodi Francesca , Giustezza Francesca , Postiglione Alfredo , Pappatà Sabina , Ferrari Raffaele , Bossù Paola , Angiolillo Antonella , di Costanzo Alfonso , Caiazzo Massimiliano , Vitale Emilia
Published: July 2020

Sialic acid-binding immunoglobulin-type lectins, which are predominantly expressed in immune cells, represent a family of immunomodulatory receptors with inhibitory and activating signals, in both healthy and disease states. Genetic factors are important in all forms of dementia, especially in early onset dementia. CD33 was recently recognized as a genetic risk factor for Alzheimer disease (AD). Here, we present a 2-generation family with 4 members, the father and the 3 siblings, characterized by an early form of unusual dementia exhibiting a behavioral variant close to behavioral variant frontotemporal dementia phenotype and severe forms of memory loss suggestive of AD. We analyzed the CD33 gene in this family and identified 10 single nucleotide polymorphisms (SNPs) in a linkage disequilibrium block associated with the disease. We also identified a tag SNP, rs2455069-A>G, in CD33 exon 2 that could be involved with dementia risk. Additionally, we excluded the presence of C9orf72 expansion mutations and other mutations previously associated with sporadic FTD and AD. The tag SNP association was also analyzed in selected sporadic AD patients from the same Southern Italy region. We demonstrate that CD33 and SIGLECL1 have a significantly increased level of expression in these patients.
Expression of Mutant Ubiquitin and Proteostasis Impairment in Kii Amyotrophic Lateral Sclerosis/Parkinsonism-Dementia Complex Brains
Authors: Verheijen Bert M , Morimoto Satoru , Sasaki Ryogen , Oyanagi Kiyomitsu , Kokubo Yasumasa , Kuzuhara Shigeki , van Leeuwen Fred W
Published: July 2020

Kii amyotrophic lateral sclerosis/parkinsonism-dementia complex (ALS/PDC) is a progressive neurodegenerative disorder that is endemic to the Kii peninsula of Japan. The disorder is clinically characterized by a variable combination of parkinsonism, dementia, and motor neuron symptoms. Despite extensive investigations, the etiology and pathogenesis of ALS/PDC remain unclear. At the neuropathological level, Kii ALS/PDC is characterized by neuronal loss and tau-dominant polyproteinopathy. Here, we report the accumulation of several proteins involved in protein homeostasis pathways, that is, the ubiquitin-proteasome system and the autophagy-lysosome pathway, in postmortem brain tissue from a number of Kii ALS/PDC cases (n = 4). Of particular interest is the presence of a mutant ubiquitin protein (UBB+1), which is indicative of disrupted ubiquitin homeostasis. The findings suggest that abnormal protein aggregation is linked to impaired protein homeostasis pathways in Kii ALS/PDC.
The Multipotential of Leucine-Rich α-2 Glycoprotein 1 as a Clinicopathological Biomarker of Glioblastoma
Authors: Furuta Takuya , Sugita Yasuo , Komaki Satoru , Ohshima Koichi , Morioka Motohiro , Uchida Yasuo , Tachikawa Masanori , Ohtsuki Sumio , Terasaki Tetsuya , Nakada Mitsutoshi
Published: July 2020

Leucine-rich α-2 glycoprotein 1 (LRG1) is a diagnostic marker candidate for glioblastoma. Although LRG1 has been associated with angiogenesis, it has been suggested that its biomarker role differs depending on the type of tumor. In this study, a clinicopathological examination of LRG1’s role as a biomarker for glioblastoma was performed. We used tumor tissues of 155 cases with diffuse gliomas (27 astrocytomas, 14 oligodendrogliomas, 114 glioblastomas). The immunohistochemical LRG1 intensity scoring was classified into 2 groups: low expression and high expression. Mutations of IDH1, IDH2, and TERT promoter were analyzed through the Sanger method. We examined the relationship between LRG1 expression level in glioblastoma and clinical parameters, such as age, preoperative Karnofsky performance status, tumor location, extent of resection, O6-methylguanine DNA methyltransferase promoter, and prognosis. LRG1 high expression rate was 41.2% in glioblastoma, 3.7% in astrocytoma, and 21.4% in oligodendroglioma. Glioblastoma showed a significantly higher LRG1 expression than lower-grade glioma (p = 0.0003). High expression of LRG1 was an independent favorable prognostic factor (p = 0.019) in IDH-wildtype glioblastoma and correlated with gross total resection (p = 0.002) and the tumor location on nonsubventricular zone (p = 0.00007). LRG1 demonstrated multiple potential as a diagnostic, prognostic, and regional biomarker for glioblastoma.
Species-specific responses to drought, salinity and their interactions in Populus euphratica and P. pruinosa seedlings
Authors: Yu Lei , Dong Haojie , Li Zhijun , Han Zhanjiang , Korpelainen Helena , Li Chunyang
Published: July 2020

Aims

Drought and salinity are severe abiotic stress factors, which limit plant growth and productivity, particularly in desert regions. In this study, we employed two desert poplars, Populus euphratica Oliver and Populus pruinosa Schrenk seedlings, to compare their tolerance to drought, salinity and combined stress.

Methods

We investigated species-specific responses of P. euphratica and P. pruinosa in growth, photosynthetic capacity and pigment contents, nonstructural carbohydrate concentrations, Cl− allocation, osmotic regulation and the accumulation of reactive oxygen species (ROS) under drought, salinity and the combined stress.

Important Findings

Populus pruinosa exhibited greater growth inhibitory effects, photosynthesis decline, stomatal closure and ROS accumulation, and lower antioxidant enzyme activities and osmotic regulation compared with P. euphratica under drought, salinity and especially under their combined stress. On the other hand, salt-stressed P. euphratica plants restricted salt transportation from roots to leaves, and allocated more Cl− to coarse roots and less to leaves, whereas salt-stressed P. pruinosa allocated more Cl− to leaves. It was shown that there is species-specific variation in these two desert poplars, and P. pruinosa suffers greater negative effects compared with P. euphratica under drought, salinity and especially under the combined stress. Therefore, in ecological restoration and afforestation efforts, species-specific responses and tolerances of these two poplar species to drought and salinity should be considered under climate change with increasing drought and soil salinity developing.
Minimal incision superficialization of the brachial artery: a technical note
Authors: Kawatani Yohei , Oguri Atsushi
Published: July 2020

The Japanese Society for Dialysis Therapy recommends superficialization of the brachial artery (BA) for vascular access in patients with comorbidities. We describe a novel minimal incision superficialization surgery of a BA through a single small incision. A 78-year-old male, who underwent chronic hemodialysis through an arterio-venous fistula, was transferred to our hospital for treatment of heart failure. We chose superficialization of the right BA for new vascular access. Under tumescent local analgesia, though a single 2-cm long incision, the BA was superficialized for 10-cm long. To complete procedures in the narrow and deep space, vessel branches were ligated by vascular clip and knot-less barbed suture was applied for closure of the brachial fascia beneath the BA. The hemodynamic status during the hemodialysis improved and the New York Heat Association (NYHA) classification grade improved from IV to II. This technique can be an alternative for arterio-venous fistula in patients with comorbidities.
Root architecture for improved resource capture: trade-offs in complex environments
Authors: van der Bom Frederik J T , Williams Alwyn , Bell Michael J
Published: July 2020

Root architecture is a promising breeding target for developing resource-efficient crops. Breeders and plant physiologists have called for root ideotypes that have narrow, deep root systems for improved water and nitrate capture, or wide, shallower root systems for better uptake of less mobile topsoil nutrients such as phosphorus. Yet evidence of relationships between root architecture and crop yield is limited. Many studies focus on the response to a single constraint, despite the fact that crops are frequently exposed to multiple soil constraints. For example, in dryland soils under no-till management, topsoil nutrient stratification is an emergent profile characteristic, leading to spatial separation of water and nutrients as the soil profile dries. This results in spatio-temporal trade-offs between efficient resource capture and pre-defined root ideotypes developed to counter a single constraint. We believe there is need to identify and better understand trade-offs involved in the efficient capture of multiple, spatially disjunct soil resources. Additionally, how these trade-offs interact with genotype (root architecture), environment (soil constraints), and management (agronomy) are critical unknowns. We argue that identifying root traits that enable efficient capture of multiple soil resources under fluctuating environmental constraints is a key step towards meeting the challenges of global food security.
Germany’s integration politics in practice: Lived realities of Chinese-speaking skilled female family migrants
Authors: Hsu Chieh
Published: July 2020

Germany imposes integration-oriented regulations before and after entry on third-country family migrants. However, little attention has been paid to how integration actually unfolds for the subjects of these regulations, usually women, who are situated in the private sphere of the family. This article examines how the concept of integration, a state-anticipated and state-stipulated goal, is connected to the early adaptation of skilled female family migrants who are married to German husbands. Based on participant observation and semi-structured interviews with 21 Chinese-speaking women across Germany, I focus on how these women mobilize resources to overcome ‘hurdles’ of entry requirements, how they define and interpret their tasks and obligation of integration upon arrival, and how the dynamics of their intercultural marriage affect their integration. Viewing these female spousal migrants as subjects in an integration-oriented family migration regime that legitimates intensive integration also in the private sphere, this article highlights these women’s ‘wife’ and ‘migrant’ roles in the family. Specifically, through interactions with their significant others and extended family members, role-related expectations are transferred. The results show that female spouses are consequently exposed to an alternative ideal of integration that is tied to their domestic role. This homebound notion of integration differs from the social integration envisioned and coveted by the German government. This research broadly unveils how these skilled female family migrants’ early acculturation can be seen as a rivalry between pre-established self-conceptions and new circumstances in the host society.
Necrosis and Brain Invasion Predict Radio-Resistance and Tumor Recurrence in Atypical Meningioma: A Retrospective Cohort Study
Authors: Garcia-Segura Monica Emili , Erickson Anders Wilder , Jairath Rishi , Munoz David G , Das Sunit
Published: July 2020

BACKGROUND

Meningiomas are the most common tumors occurring in the central nervous system, with variable recurrence rates depending on World Health Organization grading. Atypical (Grade II) meningioma has a higher rate of recurrence than benign (Grade I) meningioma. The efficacy of adjuvant radiotherapy (RT) to improve tumor control has been questioned.

OBJECTIVE

To investigate clinical and histopathological predictors of tumor recurrence and radio-resistance in atypical meningiomas.

METHODS

This cohort study retrospectively reviewed all patients in St. Michael's Hospital CNS tumor patient database who underwent surgical resection of a Grade II meningioma from 1995 to 2015. Cases with neurofibromatosis type II, multiple satellite tumors, spinal cord meningioma, radiation-induced meningioma, and perioperative death were excluded. Patient demographics, neuropathological diagnosis, tumor location, extent of resection, radiation therapy, and time to recurrence or progression were recorded. Cox univariate regression and Kaplan-Meier survival analysis were employed to identify risk factors for recurrence and radio-resistance.

RESULTS

Among 181 patients, the combination of necrosis and brain invasion was associated with an increased recurrence risk (hazard ratio [HR] = 4.560, P = .001) and the lowest progression-free survival (PFS) relative to other pathological predictors. This trend was maintained after gross total resection (GTR, P = .001). RT was associated with decreased PFS (P = .001), even in patients who received GTR (P = .001).

CONCLUSION

The combination of necrosis and brain invasion is a strong predictor of tumor recurrence and radio-resistance in meningioma, regardless of EOR or adjuvant RT. Our findings question the sensibility of brain invasion as an absolute criterion for Grade II status.
Income and education inequalities in cervical cancer incidence in Canada, 1992–2010
Authors: Morriscey Carol , Hajizadeh Mohammad
Published: July 2020

Background

There is evidence of socioeconomic inequalities in cancer incidence in Canada and other countries globally, yet there is no study investigating socioeconomic inequalities in national cervical cancer incidence in Canada. Thus, the current study investigated income and education inequalities in the incidence of cervical cancer in Canada from 1992 to 2010.

Methods

Data were derived from a linked dataset that combined cervical cancer incidence from the Canadian Cancer Registry and demographic and socioeconomic information from the Canadian Census of Population and the National Household Survey. The Concentration index approach was used to measure income and education inequalities in the incidence of cervical cancer over time.

Results

National incidence of cervical cancer decreased significantly from 1992 to 2010. The age-standardized C was negative for the majority of years for both income and education inequalities, but the preponderance were not significant. Trend analyses of socioeconomic inequalities suggested an increasing concentration of cervical cancer incidence among less-educated females over the study period.

Conclusions

Over almost two decades, there were no pervasive socioeconomic inequalities in the incidence of cervical cancer in Canada. As such, policies aimed at reducing the incidence of cervical cancer should focus on the general population, irrespective of socioeconomic status.
A randomized prospective open-label controlled trial comparing the performance of a connected monitoring interface versus physical routine monitoring in patients with rheumatoid arthritis
Authors: Pers Yves-Marie , Valsecchi Verushka , Mura Thibault , Aouinti Safa , Filippi Nathalie , Marouen Sarah , Letaief Hind , Le Blay Pierre , Autuori Michel , Fournet Dominique , Mercier Grégoire , Ferreira Rosanna , Jorgensen Christian
Published: July 2020

Objectives

In RA, telemedicine may allow tight control of disease activity while reducing hospital visits. We developed a smartphone application connected with a physician’s interface to monitor RA patients. We aimed to assess the performance of this e-Health solution in comparison with routine practice in the management of patients with RA.

Methods

A six-month pragmatic, randomized, controlled, prospective, clinical trial was conducted in RA patients with high to moderate disease activity starting a new DMARD therapy. Two groups were established: ‘connected monitoring’ and ‘conventional monitoring’. The primary outcome was the number of physical visits between baseline and six months. Secondary outcomes included adherence, satisfaction, changes in clinical, functional and health status scores (Short-Form 12).

Results

Of the 94 randomized patients, 89 completed study: 44 in the ‘conventional monitoring’ arm and 45 in the ‘connected monitoring’ arm. The total number of physical visits between required baseline and six-month visits was significantly lower in the ‘connected monitoring’ group [0.42 (0.58) vs 1.93 (0.55); P <0.05]. No differences between groups were observed in the clinical and functional scores. A better quality of life for Short-Form 12 subscores (Role-Physical and Role-Emotional) were found in the ‘connected monitoring’ group.

Conclusion

Our results suggest that connected monitoring reduces the number of physical visits while maintaining a tight control of disease activity and improving quality of life in patients with RA starting a new treatment.

Trial registration

ClinicalTrials.gov, https://clinicaltrials.gov, NCT03005925.
Genetics and the axial spondyloarthritis spectrum
Authors: Brown Matthew A , Xu Huji , Li Zhixiu
Published: July 2020

The axial SpAs (axSpAs) are clearly clinically a heterogeneous set of diseases with markedly varying extra-articular features. These diseases are all highly heritable and have overlapping but differing genetic origins. Shared features include association with HLA class I alleles and genes of the IL-23 pathway, among other things. Significant differences do exist however, both in the genetic loci involved and at specific loci in the individual genetic variants associated with each disease. These similarities and differences are of great interest in regards to disease pathogenesis and treatment development, although individually they are too small in effect to be of prognostic or diagnostic value. Polygenic risk scores, which capture a high proportion of the genetic variation between disorders, have been shown to have clinically useful discriminatory capacity in axSpA. This suggests they have the potential to enable improved disease classification, incorporating basic pathogenic features such as genomics, and ultimately benefitting clinical care. The aim of this article is to review the genetic characteristics of the spectrum of axSpAs and to discuss how this influences our understanding of the disease pathogenesis and the clinical implications of this understanding.
Natural Defect of a Plant Rhabdovirus Glycoprotein Gene: A Case Study of Virus–Plant Coevolution
Authors: Zhang Song , Huang Aijun , Zhou Xin , Li Zhenghe , Dietzgen Ralf G. , Zhou Changyong , Cao Mengji
Published: July 2020

Seven isolates of a putative cytorhabdovirus (family Rhabdoviridae, order Mononegavirales) designated as citrus-associated rhabdovirus (CiaRV) were identified in citrus, passion fruit, and paper bush from the same geographical area in China. CiaRV, bean-associated cytorhabdovirus (Brazil), and papaya virus E (Ecuador) should be taxonomically classified in the species Papaya cytorhabdovirus. Due to natural mutations, the glycoprotein (G) and P4 genes were impaired in citrus-infecting isolates of CiaRV, resulting in an atypical rhabdovirus genome organization of 3′ leader-N-P-P3-M-L-5′ trailer. The P3 protein of CiaRV shared a common origin with begomoviral movement proteins (family Geminiviridae). Secondary structure analysis and trans-complementation of movement-deficient tomato mosaic virus and potato virus X mutants by CiaRV P3 supported its function in viral cell-to-cell trafficking. The wide geographical dispersal of CiaRV and related viruses suggests an efficient transmission mechanism, as well as an underlying risk to global agriculture. Both the natural phenomenon and experimental analyses demonstrated presence of the “degraded” type of CiaRV in citrus, in parallel to “undegraded” types in other host plant species. This case study shows a plant virus losing the function of an important but nonessential gene, likely due to host shift and adaption, which deepened our understanding of course of natural viral diversification.
A High-Quality Genome Resource of Botrytis fragariae, a New and Rapidly Spreading Fungal Pathogen Causing Strawberry Gray Mold in the United States
Authors: Wu Ying , Saski Christopher , Schnabel Guido , Xiao Shunyuan , Hu Mengjun
Published: July 2020

Although Botrytis fragariae was only recently identified as a new Botrytis species that causes strawberry gray mold, its prevalence extends to many states of the eastern United States. Compared with B. cinerea, which is known to be the causal agent of gray mold on many crop plants including strawberry, B. fragariae appears to have specifically adapted to strawberry and exhibits distinct fungicide sensitivity. This is the first presentation of a high-quality genome assembly of B. fragariae with gene annotation based on sequence homology and deep transcriptome data. The genome sequence information from B. fragariae is expected to help reveal genomic features underlying its host specialization and evolution of distinct fungicide resistance and other novel pathogenicity mechanisms.
Organoid in colorectal cancer: progress and challenges
Authors: Ji Deng-Bo , Wu Ai-Wen
Published: July 2020

Patient-derived tumor organoids (PDOs) currently represent important modeling tools in pre-clinical investigation of malignancies. Organoid cultures conserve the genetic and phenotypic characteristics of the original tumor and maintain its heterogeneity, allowing their application in many research fields. PDOs derived from colorectal cancer (CRC) have been used for genetic modeling to investigate the function of driver genes. Some researchers have been exploring the value of CRC PDOs in chemotherapy, targeted therapy, and radiotherapy response prediction. The successful generation of PDOs derived from CRC could deepen our understanding of CRC biology and provide novel tools for cancer modeling, for realizing precision medicine by assessing specimens from individual patients ex vivo. The present review discusses recently reported advances in CRC PDOs and the challenges they face as pre-clinical models in CRC research.
Intestinal epithelial chemokine (C-C motif) ligand 7 overexpression protects against high fat diet-induced obesity and hepatic steatosis in mice
Authors: Luo Zhi-Hong , Niu Meng-Wei , Gong Shen-Hai , Wu Guang-Yan , Wang Teng , Wang Fang-Zhao , Wei Guo-Quan , He Zhan-Ke , Jiang Yong , Chen Peng
Published: July 2020

Background

We previously found that the intestinal epithelial chemokine (C-C motif) ligand 7 (CCL7) plays an important role in the development of toxin-induced acute liver damage. The detailed effects of intestinal epithelial CCL7 on chronic diseases; however, are still unclear. Here, we aimed to investigate the impact of intestinal epithelial CCL7 overexpression on high-fat diet (HFD)-induced obesity and steatohepatitis in mice.

Methods

Intestinal epithelial CCL7 overexpression (CCL7

tgIEC

) mice and their wild-type (WT) littermates were fed with normal chow or HFD for 16 weeks to induce obesity and non-alcoholic fatty liver disease. Body weight gain, as well as adipose tissue index were assessed. Liver injury was monitored by histological analysis and real time polymerase chain reaction. Gut microbial composition was analyzed by 16S rRNA gene sequencing.

Results

We found that the CCL7

tgIEC

mice on a HFD had markedly decreased weight gain (8.9 vs. 17.0 g, P < 0.05) and a lower adipose tissue index that include mesenteric fat (1.0% vs. 1.76%, P < 0.05), gonadal fat (2.1% vs. 6.1%, P < 0.05), subcutaneous fat (1.0% vs. 2.8%, P < 0.05) compared to WT animals. HFD-induced glucose intolerance and insulin resistance were also significantly improved in CCL7

tgIEC

mice compared to WT. Furthermore, HFD-fed CCL7

tgIEC

mice displayed less hepatic lipid accumulation and lower expression of inflammatory factors than WT mice. 16S rRNA gene sequencing demonstrated that CCL7 overexpression in intestinal epithelial cells improved HFD-induced gut microbial dysbiosis.

Conclusions

Our study revealed that CCL7 overexpression in the intestinal epithelium protects mice against the progression of diet-induced obesity, hepatic steatosis, and enteric dysbiosis.
Radiological features of traumatic vertebral endplate fracture: an analysis of 194 cases with 263 vertebral fractures
Authors: Wang Xiao-Rong , Xu Fei-Rong , Huang Qiu-Li , Wáng Yì Xiáng J.
Published: July 2020

Background

The importance of identifying osteoporotic vertebral endplate or/and cortex fracture (ECF), which primarily includes endplate fracture (EPF) and vertebral anterior cortex buckling, has been recognized. However, some old traumatic ECFs with healing process in the elderly may be mistaken as osteoporotic. This study analyzes the radiological features of traumatic EPF.

Methods

This was a retrospective analysis of 194 spine trauma patients with 263 vertebral fractures (mean age: 42.11 ± 9.82 years, 118 males and 76 females). All patients had traumatic EPF identified by X-ray/CT/MRI.

Results

The involved vertebra was mostly L1 (29.7%), followed by T12 and L2. Except EPFs involved both superior and inferior endplates (12.6%), only 1.9% involved inferior endplate alone, with the majority involved superior endplate. If each endplate was divided into five segments of equal lengths (from anterior to posterior: a1, a2, m, p2, p1), the most depressed point of superior EPFs was mostly at segment-a2 (approximately 45%), followed by segment-a1 (approximately 20%) or segment-m (approximately 20%), and very rarely at segment-p1. The upper 1/3 of anterior vertebral wall was more likely to fracture, followed by middle 1/3 of anterior wall. For posterior vertebral wall fracture, 68.5% broke the bony wall surrounding the basivertebral vain. 58.6%, 30.0%, and 11.4% of vertebral fractures had <1/5, 1/5–1/3, and >1/3 vertebral body height loss. As the extent of vertebral height loss increased, the chance of having both superior and inferior EPFs also increased; however, the chance of having inferior EPF alone did not increase.

Conclusion

Traumatic EPF features are characterized, which may help the differentiation of traumatic and osteoporotic EPFs.
Oxcarbazepine monotherapy in children with benign epilepsy with centrotemporal spikes improves quality of life
Authors: Liu Wan-Ting , Yan Xiu-Xian , Cheng Da-Zhi , Zhang Han-Zi , Ding Ning , Xu Ke-Ming , Zhou Xin-Lin , Chen Qian
Published: July 2020

Background

Benign epilepsy with centrotemporal spikes (BECTS) is the most common type of childhood idiopathic focal epilepsy. BECTS is associated with pervasive cognitive deficits and behavior problems. While seizures can be easily controlled, it is crucial to select anti-epileptic drugs that do not impair cognition, do not cause psychosocial effects, and improve the quality of life. Previous studies showed effects of oxcarbazepine (OXC) monotherapy on the cognitive and psychosocial profiles of patients with BECTS. Here, we studied the effects of OXC monotherapy on the neuropsychologic profiles and quality of life in patients with BECTS in China.

Methods

Thirty-one patients aged 6 to 12 years newly diagnosed with BECTS were recruited. A psychometric assessment was performed before and during the follow-up of OXC monotherapy with Cognitive Computerized Task Battery, Depression Self-Rating Scale for children, Screen for Child Anxiety Related Emotional Disorders, and Quality of Life in Epilepsy-31 (QOLIE-31). The results of the assessments were compared to explore the effect of OXC monotherapy in patients with BECTS.

Results

Thirty children with BECTS completed the study. Five of ten cognitive test scores improved after treatment via OXC monotherapy, including visual tracing (F = 14.480, P < 0.001), paired associated learning (language) (F = 6.292, P < 0.001), paired associated learning (number) (F = 9.721, P < 0.05), word semantic (F = 6.003, P < 0.05), and simple subtraction (F = 6.229, P < 0.05). Of the neuropsychology data concerning the quality of life, statistically significant improvements were observed in emotion (F = 4.946, P < 0.05), QOLIE-social (F = 5.912, P < 0.05), and QOLIE-total (F = 14.161, P < 0.001).

Conclusions

OXC is safe and does not impair neuropsychologic functions, with no obvious mood burden on children with BECTS. Most importantly, OXC has positive impacts on children's perception of quality of life, especially in terms of happiness and life satisfaction.
Potential contribution of the gut microbiota to hypoglycemia after gastric bypass surgery
Authors: Zhou Li-Yuan , Deng Ming-Qun , Xiao Xin-Hua
Published: July 2020

Obesity has become a global health problem. Lifestyle modification and medical treatment only appear to yield short-term weight loss. Roux-en-Y gastric bypass (RYGB) is the most popular bariatric procedure, and it sustains weight reduction and results in the remission of obesity-associated comorbidities for obese individuals. However, patients who undergo this surgery may develop hypoglycemia. To date, the diagnosis is challenging and the prevalence of post-RYGB hypoglycemia (PRH) is unclear. RYGB alters the anatomy of the upper gastrointestinal tract and has a combined effect of caloric intake restriction and nutrient malabsorption. Nevertheless, the physiologic changes after RYGB are complex. Although hyperinsulinemia, incretin effects, dysfunction of β-cells and α-cells, and some other factors have been widely investigated and are reported to be possible mediators of PRH, the pathogenesis is still not completely understood. In light of the important role of the gut microbiome in metabolism, we hypothesized that the gut microbiome might also be a critical link between RYGB and hypoglycemia. In this review, we mainly highlight the current possible factors predisposing individuals to PRH, particularly related to the gut microbiota, which may yield significant insights into the intestinal regulation of glucose metabolic homeostasis and provide novel clues to improve the treatment of type 2 diabetes mellitus.
Mitral regurgitation: a contemporary review of percutaneous mitral valve repair and role of periprocedural imaging
Authors: Beg Faheemullah , Little Stephen H. , Faza Nadeen N.
Published: July 2020

Purpose of review

This article aims to review recent landmark clinical trials that have resulted in a paradigm shift in the management of patients with mitral regurgitation. This article additionally highlights the instrumental role that structural heart disease (SHD) imaging plays in evaluation of mitral regurgitation and determining candidacy for transcatheter mitral valve repair (TMVr), in addition to the procedural guidance and detection of complications.

Recent findings

Edge-to-edge TMVr with the MitraClip device (Abbott Vascular, Santa Clara, California) was initially studied and subsequently approved by the US Food and Drug Administration (FDA) for treatment of primary mitral regurgitation in 2013. After the publication of a landmark clinical trial in 2018, the indications have been expanded and FDA has subsequently, in 2019, approved TMVr for patients with functional mitral regurgitation. This has been paralleled by advances in cardiac imaging with more emphasis on the role of SHD imagers as a part of the heart team.

TMVr has revolutionized the management of mitral regurgitation and relies heavily on SHD imaging to ensure optimal outcomes.
The evolving role of coronary artery calcium in preventive cardiology 30 years after the Agatston score
Authors: Kapoor Karan , Cainzos-Achirica Miguel , Nasir Khurram
Published: July 2020

Purpose of review

On the brink of the 30th anniversary of the Agatston score we summarize the epidemiological data that shaped the recommendations relevant to coronary artery calcium (CAC) included in the 2018/2019 US and European guidelines for the primary prevention of atherosclerotic cardiovascular disease (ASCVD). We also discuss the implications of novel CAC research conducted in asymptomatic populations within the past 2 years.

Recent findings

Based on a wealth of observational evidence, CAC has emerged as a mainstay in personalized risk assessment and is now endorsed as a class IIa tool in both US and European guidelines. In the past 2 years, data supporting the prognostic power of CAC has kept mounting, with longer term follow-up data now available. CAC has been evaluated in a variety of patient populations including individuals with severe hypercholesterolemia, diabetes mellitus and younger adults with family history of ASCVD, in all of whom it may be able to inform a more personalized management. Novel CAC scoring approaches are also discussed.

Despite a strong endorsement in recent guidelines, active research in the last 2 years has provided further insights on the potential utility of CAC in informing a more individualized preventive management in broader populations.
Vascular consequences of inflammation: a position statement from the ESH Working Group on Vascular Structure and Function and the ARTERY Society
Authors: Zanoli Luca , Briet Marie , Empana Jean P. , Cunha Pedro G. , Mäki-Petäjä Kaisa M. , Protogerou Athanase D. , Tedgui Alain , Touyz Rhian M. , Schiffrin Ernesto L. , Spronck Bart , Bouchard Philippe , Vlachopoulos Charalambos , Bruno Rosa M. , Boutouyrie Pierre
Published: July 2020

Inflammation is a physiological response to aggression of pathogenic agents aimed at eliminating the aggressor agent and promoting healing. Excessive inflammation, however, may contribute to tissue damage and an alteration of arterial structure and function. Increased arterial stiffness is a well recognized cardiovascular risk factor independent of blood pressure levels and an intermediate endpoint for cardiovascular events. In the present review, we discuss immune-mediated mechanisms by which inflammation can influence arterial physiology and lead to vascular dysfunction such as atherosclerosis and arterial stiffening. We also show that acute inflammation predisposes the vasculature to arterial dysfunction and stiffening, and alteration of endothelial function and that chronic inflammatory diseases such as rheumatoid arthritis, inflammatory bowel disease and psoriasis are accompanied by profound arterial dysfunction which is proportional to the severity of inflammation. Current findings suggest that treatment of inflammation by targeted drugs leads to regression of arterial dysfunction. There is hope that these treatments will improve outcomes for patients.
Beta-blockers in hypertension: overview and meta-analysis of randomized outcome trials
Authors: Thomopoulos Costas , Bazoukis George , Tsioufis Costas , Mancia Giuseppe
Published: July 2020

Background:

Meta-analyses from randomized outcome-based trials have challenged the role of beta-blockers for the treatment of hypertension. However, because they often include trials on diseases other than hypertension, the role of these drugs in the choice of the blood pressure (BP)-lowering treatment strategies remains unclear.

Methods:

Electronic databases were searched for randomized trials that compared beta-blockers vs. placebo/no-treatment/less-intense treatment (BP-lowering trials) or beta-blockers vs. other antihypertensive agents in patients with or without hypertension (comparison trials). Among BP-lowering trials and according to baseline comorbidity, we separately considered trials in hypertension, trials without chronic heart failure or acute myocardial infarction, and trials with either chronic heart failure or acute myocardial infarction. Seven fatal and nonfatal outcomes were calculated (random-effects model) for BP-lowering or comparison trials.

Results:

A total of 84 BP-lowering or comparison trials (165 850 patients) were eligible. In 67 BP-lowering trials (68 478 patients; mean follow-up 2.5 years; baseline SBP/DBP, 136/82 mmHg), beta blockers were associated with a lower incidence of major cardiovascular events [risk ratio 0.85 and 95% confidence interval (95% CI) 0.78–0.92] and all-cause death (risk ratio 0.81 and 95% CI 0.75–0.86). Restriction of the analysis to five trials recruiting exclusively hypertensive patients (18 724 patients; mean follow-up 5.1 years; baseline SBP/DBP 163/94 mmHg), a -10.5/-7.0 mmHg BP decrease was accompanied by reduction of major cardiovascular events by 22% (95% CI, 6–34). In 24 comparison trials (103 764 patients, 3.92 years of mean follow-up), beta-blockers compared with other agents were less protective for stroke and all-cause death in all trials and in trials conducted exclusively in hypertensive patients (averaged risk ratio increase 20 and 6%, respectively, for both cases).

Conclusion:

Compared with other antihypertensive agents, beta-blockers appear to be substantially less protective against stroke and overall mortality. However, they exhibit a substantial risk-reducing ability for all events when prescribed to lower BP in patients with modest or more clear BP elevations, and therefore can be used as additional agents in hypertensive patients.