bluuebunny/crossref_metadata_2025 huggingface dataset:
Data from Long Noncoding RNA NEAT1 Acts as a Molecular Switch for BRD4 Transcriptional Activity and Mediates Repression of BRD4/WDR5 Target Genes
Authors: Pistoni Mariaelena , Rossi Teresa , Donati Benedetta , Torricelli Federica , Polano Maurizio , Ciarrocchi Alessia
Published: April 2023

Abstract
BRD4 is an epigenome reader known to exert key roles at the interface between chromatin remodeling and transcriptional regulation, and is primarily known for its role in promoting gene expression. In selective contexts, however, BRD4 may work as negative regulator of transcription. Here, we reported that BRD4 binds several long noncoding RNAs (lncRNA). Among these, the lncRNA NEAT1 was found to interfere with BRD4 transcriptional activity. Mechanistically, lncNEAT1 forms a complex with BRD4 and WDR5 and maintains them in a low-activity state. Treatment with Bromodomains and Extraterminal (BET) inhibitor caused the lncRNA NEAT1 to dissociate from the BRD4/WDR5 complex, restored the acetyl-transferase capacity of BRD4, and restored the availability of WDR5 to promote histone trimethylation, thereby promoting BRD4/WDR5 transcriptional activity and activation of target gene expression. In addition, the lncRNA NEAT1 then became available to bind and to inhibit EZH2, cooperatively increasing transcriptional activation.
Implications:
Our results revealed an epigenetic program that involves the interaction between the lncRNA NEAT1 and BRD4, functioning as a molecular switch between BRD4's activator and repressor chromatin complexes.
Data from Intraperitoneal Oil Application Causes Local Inflammation with Depletion of Resident Peritoneal Macrophages
Authors: Alsina-Sanchis Elisenda , Mülfarth Ronja , Moll Iris , Mogler Carolin , Rodriguez-Vita Juan , Fischer Andreas
Published: April 2023

Abstract
Oil is frequently used as a solvent to inject lipophilic substances into the peritoneum of laboratory animals. Although mineral oil causes chronic peritoneal inflammation, little is known whether other oils are better suited. We show that olive, peanut, corn, or mineral oil causes xanthogranulomatous inflammation with depletion of resident peritoneal macrophages. However, there were striking differences in the severity of the inflammatory response. Peanut and mineral oil caused severe chronic inflammation with persistent neutrophil and monocyte recruitment, expansion of the vasculature, and fibrosis. Corn and olive oil provoked no or only mild signs of chronic inflammation. Mechanistically, the vegetal oils were taken up by macrophages leading to foam cell formation and induction of cell death. Olive oil triggered caspase-3 cleavage and apoptosis, which facilitate the resolution of inflammation. Peanut oil and, to a lesser degree, corn oil, triggered caspase-1 activation and macrophage pyroptosis, which impair the resolution of inflammation. As such, intraperitoneal oil administration can interfere with the outcome of subsequent experiments. As a proof of principle, intraperitoneal peanut oil injection was compared with its oral delivery in a thioglycolate-induced peritonitis model. The chronic peritoneal inflammation due to peanut oil injection impeded the proper recruitment of macrophages and the resolution of inflammation in this peritonitis model. In summary, the data indicate that it is advisable to deliver lipophilic substances, like tamoxifen, by oral gavage instead of intraperitoneal injection.
Implications:
This work contributes to the reproducibility of animal research by helping to understand some of the undesired effects observed in animal experiments.
Data from SOHLH2 Suppresses Angiogenesis by Downregulating HIF1α Expression in Breast Cancer
Authors: Cui Weiwei , Xiao Yunling , Zhang Ruihong , Zhao Na , Zhang Xianghong , Wang Fuwu , Liu Yang , Zhang Xiaoli , Hao Jing
Published: April 2023

Abstract
SOHLH2 has been demonstrated the downregulation in various cancers and the involvement in tumor growth and metastasis. However, the function of SOHLH2 on tumor angiogenesis and the underlying molecular mechanisms have not been interrogated. IHC staining results revealed that SOHLH2 was negatively associated with microvessel density (MVD), tumor size, histology grade, and metastasis. Overexpression of SOHLH2 inhibited the angiogenic behavior of human umbilical vein endothelial cells (HUVEC) by a tumor cell–mediated paracrine signal, while knockdown of SOHLH2 promoted HUVEC angiogenic behavior. Ectopic SOHLH2 expression remarkably suppressed tumor growth and MVD in xenograft tumors, downregulated the expression of hypoxia inducible factor-1 alpha (HIF1α)-mediated proangiogenic genes in vivo and in vitro, while knockdown of SOHLH2 had an opposite result. Furthermore, we found that upregulation of HIF1α reversed SOHLH2-induced suppression of breast cancer angiogenesis, while KC7F2, the inhibitor of HIF1α, could attenuate the promotion of angiogenesis by SOHLH2 silencing. Using Chromatin immunoprecipitation and luciferase reporter assays, we validated that SOHLH2 could directly bind to HIF1α promoter and repress its transcriptional activity. Collectively, SOHLH2 suppresses breast cancer angiogenesis by downregulating HIF1α transcription and may be a potential biomarker for anti-angiogenesis therapy.
Implications:
SOHLH2 directly represses HIF1α–mediated angiogenesis and serves as an important inhibitor of angiogenesis in breast cancer.
Data from EGR1 Addiction in Diffuse Large B-cell Lymphoma
Authors: Kimpara Shuichi , Lu Li , Hoang Nguyet M. , Zhu Fen , Bates Paul D. , Daenthanasanmak Anusara , Zhang Shanxiang , Yang David T. , Kelm Amanda , Liu Yunxia , Li Yangguang , Rosiejka Alexander , Kondapelli Apoorv , Bebel Samantha , Chen Madelyn , Waldmann Thomas A. , Capitini Christian M. , Rui Lixin
Published: April 2023

Abstract
Early growth response gene (EGR1) is a transcription factor known to be a downstream effector of B-cell receptor signaling and Janus kinase 1 (JAK1) signaling in diffuse large B-cell lymphoma (DLBCL). While EGR1 is characterized as a tumor suppressor in leukemia and multiple myeloma, the role of EGR1 in lymphoma is unknown. Here we demonstrate that EGR1 is a potential oncogene that promotes cell proliferation in DLBCL. IHC analysis revealed that EGR1 expression is elevated in DLBCL compared with normal lymphoid tissues and the level of EGR1 expression is higher in activated B cell–like subtype (ABC) than germinal center B cell–like subtype (GCB). EGR1 expression is required for the survival and proliferation of DLBCL cells. Genomic analyses demonstrated that EGR1 upregulates expression of MYC and E2F pathway genes through the CBP/p300/H3K27ac/BRD4 axis while repressing expression of the type I IFN pathway genes by interaction with the corepressor NAB2. Genetic and pharmacologic inhibition of EGR1 synergizes with the BRD4 inhibitor JQ1 or the type I IFN inducer lenalidomide in growth inhibition of ABC DLBCL both in cell cultures and xenograft mouse models. Therefore, targeting oncogenic EGR1 signaling represents a potential new targeted therapeutic strategy in DLBCL, especially for the more aggressive ABC DLBCL.
Implications:
The study characterizes EGR1 as a potential oncogene that promotes cell proliferation and defines EGR1 as a new molecular target in DLBCL, the most common non-Hodgkin lymphoma.
Data from REX-1 Represses RASSF1a and Activates the MEK/ERK Pathway to Promote Tumorigenesis in Prostate Cancer
Authors: Liu Weijing , Xie An , Tu Chunhua , Liu Weipeng
Published: April 2023

Abstract
Epigenetics play an important role in the pathogenesis of prostate cancer; it is urgent to investigate vital transcription factors in methylation regulation with the aim to develop novel treatment strategies targeting prostate cancer. As a member of the zinc finger protein family, REX-1 (reduced expression-1) is a transcription factor that has been reported to be closely linked to the development of several cancers. So far, the expression level and precise function of REX-1 in prostate cancer remain largely unknown. Here, we show that REX-1 was overexpressed in prostate cancer clinical tissues, and its expression level was closely correlated with patient prognosis. REX-1 affected prostate tumor growth in vivo by MEK/ERK phosphorylation. Mechanistic studies indicated that REX-1 recruited DNMT3b (DNA methyltransferase 3b), inhibited the transcription of RASSF1a (RAS association domain family 1a), and further modulated methylation of RASSF1a promoter. Intervention of the REX-1/DNMT3b/RASSF1a axis may shed light on the development of novel therapeutic approaches for prostate cancer treatment.
Implications:
REX1 overexpression recruits DNMT3b and downregulates RASSF1a by promoter methylation, suggesting that epigenetic intervention may contribute to prostate cancer treatment.
Data from ILT3 (LILRB4) Promotes the Immunosuppressive Function of Tumor-Educated Human Monocytic Myeloid-Derived Suppressor Cells
Authors: Singh Latika , Muise Eric S. , Bhattacharya Anannya , Grein Jeff , Javaid Sarah , Stivers Peter , Zhang Jun , Qu Yujie , Joyce-Shaikh Barbara , Loboda Andrey , Zhang Chunsheng , Meehl Michael , Chiang Derek Y. , Ranganath Sheila H. , Rosenzweig Michael , Brandish Philip E.
Published: April 2023

Abstract
Myeloid-derived suppressor cells (MDSC) are immature myeloid cells that accumulate in the tumor microenvironment (TME). MDSCs have been shown to dampen antitumor immune responses and promote tumor growth; however, the mechanisms of MDSC induction and their role in promoting immune suppression in cancer remain poorly understood. Here, we characterized the phenotype and function of monocytic MDSCs (M-MDSC) generated by coculture of human peripheral blood mononuclear cells with SK-MEL-5 cancer cells in vitro. We selected the SK-MEL-5 human melanoma cell line to generate M-MDSCs because these cells form subcutaneous tumors rich in myeloid cells in humanized mice. M-MDSCs generated via SK-MEL-5 coculture expressed low levels of human leukocyte antigen (HLA)-DR, high levels of CD33 and CD11b, and suppressed both CD8+ T-cell proliferation and IFNγ secretion. M-MDSCs also expressed higher levels of immunoglobulin-like transcript 3 (ILT3, also known as LILRB4) and immunoglobulin-like transcript 4 (ILT4, also known as LILRB2) on the cell surface compared with monocytes. Therefore, we investigated how ILT3 targeting could modulate M-MDSC cell function. Treatment with an anti-ILT3 antibody impaired the acquisition of the M-MDSC suppressor phenotype and reduced the capacity of M-MDSCs to cause T-cell suppression. Finally, in combination with anti-programmed cell death protein 1 (PD1), ILT3 blockade enhanced T-cell activation as assessed by IFNγ secretion.
Implications:
These results suggest that ILT3 expressed on M-MDSCs has a role in inducing immunosuppression in cancer and that antagonism of ILT3 may be useful to reverse the immunosuppressive function of M-MDSCs and enhance the efficacy of immune checkpoint inhibitors.
Data from DNA-PK Inhibition and Radiation Promote Antitumoral Immunity through RNA Polymerase III in Pancreatic Cancer
Authors: Wang Weiwei , McMillan Matthew T. , Zhao Xinyi , Wang Zhuwen , Jiang Long , Karnak David , Lima Fatima , Parsels Joshua D. , Parsels Leslie A. , Lawrence Theodore S. , Frankel Timothy L. , Morgan Meredith A. , Green Michael D. , Zhang Qiang
Published: April 2023

Abstract
Targeting the DNA damage response in combination with radiation enhances type I interferon (T1IFN)-driven innate immune signaling. It is not understood, however, whether DNA-dependent protein kinase (DNA-PK), the kinase critical for repairing the majority of radiation-induced DNA double-strand breaks in cancer cells, is immunomodulatory. We show that combining radiation with DNA-PK inhibition increases cytosolic double-stranded DNA and tumoral T1IFN signaling in a cyclic GMP-AMP synthase (cGAS)- and stimulator of interferon genes (STING)-independent, but an RNA polymerase III (POL III), retinoic acid-inducible gene I (RIG-I), and antiviral-signaling protein (MAVS)-dependent manner. Although DNA-PK inhibition and radiation also promote programmed death-ligand 1 (PD-L1) expression, the use of anti–PD-L1 in combination with radiation and DNA-PK inhibitor potentiates antitumor immunity in pancreatic cancer models. Our findings demonstrate a novel mechanism for the antitumoral immune effects of DNA-PK inhibitor and radiation that leads to increased sensitivity to anti–PD-L1 in poorly immunogenic pancreatic cancers.
Implications:
Our work nominates a novel therapeutic strategy as well as its cellular mechanisms pertinent for future clinical trials combining M3814, radiation, and anti-PD-L1 antibody in patients with pancreatic cancer.
Data from Attenuating Adaptive VEGF-A and IL8 Signaling Restores Durable Tumor Control in AR Antagonist–Treated Prostate Cancers
Authors: Maxwell Pamela J. , McKechnie Melanie , Armstrong Christopher W. , Manley Judith M. , Ong Chee Wee , Worthington Jenny , Mills Ian G. , Longley Daniel B. , Quigley James P. , Zoubeidi Amina , de Bono Johann S. , Deryugina Elena , LaBonte Melissa J. , Waugh David J.J.
Published: April 2023

Abstract
Inhibiting androgen signaling using androgen signaling inhibitors (ASI) remains the primary treatment for castrate-resistant prostate cancer. Acquired resistance to androgen receptor (AR)-targeted therapy represents a major impediment to durable clinical response. Understanding resistance mechanisms, including the role of AR expressed in other cell types within the tumor microenvironment, will extend the clinical benefit of AR-targeted therapy. Here, we show the ASI enzalutamide induces vascular catastrophe and promotes hypoxia and microenvironment adaptation. We characterize treatment-induced hypoxia, and subsequent induction of angiogenesis, as novel mechanisms of relapse to enzalutamide, highlighting the importance of two hypoxia-regulated cytokines in underpinning relapse. We confirmed AR expression in CD34+ vascular endothelium of biopsy tissue and human vascular endothelial cells (HVEC). Enzalutamide attenuated angiogenic tubule formation and induced cytotoxicity in HVECs in vitro, and rapidly induced sustained hypoxia in LNCaP xenografts. Subsequent reoxygenation, following prolonged enzalutamide treatment, was associated with increased tumor vessel density and accelerated tumor growth. Hypoxia increased AR expression and transcriptional activity in prostate cells in vitro. Coinhibition of IL8 and VEGF-A restored tumor response in the presence of enzalutamide, confirming the functional importance of their elevated expression in enzalutamide-resistant models. Moreover, coinhibition of IL8 and VEGF-A resulted in a durable, effective resolution of enzalutamide-sensitive prostate tumors. We conclude that concurrent inhibition of two hypoxia-induced factors, IL8 and VEGF-A, prolongs tumor sensitivity to enzalutamide in preclinical models and may delay the onset of enzalutamide resistance.
Implications:
Targeting hypoxia-induced signaling may extend the therapeutic benefit of enzalutamide, providing an improved treatment strategy for patients with resistant disease.
Data from Loss of E-cadherin Induces IGF1R Activation and Reveals a Targetable Pathway in Invasive Lobular Breast Carcinoma
Authors: Elangovan Ashuvinee , Hooda Jagmohan , Savariau Laura , Puthanmadhomnarayanan Susrutha , Yates Megan E. , Chen Jian , Brown Daniel D. , McAuliffe Priscilla F. , Oesterreich Steffi , Atkinson Jennifer M. , Lee Adrian V.
Published: April 2023

Abstract
No special-type breast cancer [NST; commonly known as invasive ductal carcinoma (IDC)] and invasive lobular carcinoma (ILC) are the two major histological subtypes of breast cancer with significant differences in clinicopathological and molecular characteristics. The defining pathognomonic feature of ILC is loss of cellular adhesion protein, E-cadherin (CDH1). We have previously shown that E-cadherin functions as a negative regulator of the IGF1R and propose that E-cadherin loss in ILC sensitizes cells to growth factor signaling that thus alters their sensitivity to growth factor–signaling inhibitors and their downstream activators. To investigate this potential therapeutic vulnerability, we generated CRISPR-mediated CDH1 knockout (CDH1 KO) IDC cell lines (MCF7, T47D, and ZR75.1) to uncover the mechanism by which loss of E-cadherin results in IGF pathway activation. CDH1 KO cells demonstrated enhanced invasion and migration that was further elevated in response to IGF1, serum and collagen I. CDH1 KO cells exhibited increased sensitivity to IGF resulting in elevated downstream signaling. Despite minimal differences in membranous IGF1R levels between wild-type (WT) and CDH1 KO cells, significantly higher ligand–receptor interaction was observed in the CDH1 KO cells, potentially conferring enhanced downstream signaling activation. Critically, increased sensitivity to IGF1R, PI3K, Akt, and MEK inhibitors was observed in CDH1 KO cells and ILC patient-derived organoids.
Implications:
Overall, this suggests that these targets require further exploration in ILC treatment and that CDH1 loss may be exploited as a biomarker of response for patient stratification.
Data from Glutamine Metabolism Mediates Sensitivity to Respiratory Complex II Inhibition in Acute Myeloid Leukemia
Authors: Roma Alessia , Tcheng Matthew , Ahmed Nawaz , Walker Sarah , Jayanth Preethi , Minden Mark D. , Hope Kristin , Nekkar Rao Praveen P. , Luc Jessica , Doxey Andrew C. , Reisz Julie A. , Culp-Hill Rachel , D'Alessandro Angelo , Spagnuolo Paul A.
Published: April 2023

Abstract
Acute myeloid leukemia (AML) is a hematologic malignancy metabolically dependent on oxidative phosphorylation and mitochondrial electron transport chain (ETC) activity. AML cells are distinct from their normal hematopoietic counterparts by this metabolic reprogramming, which presents targets for new selective therapies. Here, metabolic changes in AML cells after ETC impairment are investigated. Genetic knockdown of the ETC complex II (CII) chaperone protein SDHAF1 (succinate dehydrogenase assembly factor 1) suppressed CII activity and delayed AML cell growth in vitro and in vivo. As a result, a novel small molecule that directly binds to the ubiquinone binding site of CII and inhibits its activity was identified. Pharmacologic inhibition of CII induced selective death of AML cells while sparing normal hematopoietic progenitors. Through stable isotope tracing, results show that genetic or pharmacologic inhibition of CII truncates the tricarboxylic acid cycle (TCA) and leads to anaplerotic glutamine metabolism to reestablish the truncated cycle. The inhibition of CII showed divergent fates, as AML cells lacked the metabolic plasticity to adequately utilize glutamine metabolism, resulting in preferential depletion of key TCA metabolites and death; normal cells were unaffected. These findings provide insight into the metabolic mechanisms that underlie AML's selective inhibition of CII.
Implications:
This work highlights the effects of direct CII inhibition in mediating selective AML cell death and provides insights into glutamine anaplerosis as a metabolic adaptation that can be therapeutically targeted.
Data from Redefining CD56 as a Biomarker and Therapeutic Target in Multiple Myeloma
Authors: Cottini Francesca , Rodriguez Jose , Hughes Tiffany , Sharma Nidhi , Guo Ling , Lozanski Gerard , Liu Bei , Cocucci Emanuele , Yang Yiping , Benson Don
Published: April 2023

Abstract
Multiple myeloma cells aberrantly express surface antigens compared with normal plasma cells. Among others, CD56 is present at variable levels in approximately 70% of patients with multiple myeloma; however, very little is known about CD56 role in multiple myeloma. We demonstrated that patients with multiple myeloma with more than 10% of CD56-expressing clonal multiple myeloma cells have inferior clinical outcomes. By gain-of and loss-of function models, we revealed that CD56 promotes multiple myeloma cell growth, survival, and adhesion to stromal cells. These protumoral effects are induced by the activation of the RSK2/CREB1 signaling pathway, with increased mRNA and protein levels of the anti-apoptotic genes BCL2 and MCL1. Consequently, the genomic and pharmacological inhibition of RSK2 or CREB1 specifically induced multiple myeloma cell death in CD56-expressing multiple myeloma cells. Finally, we observed that CD56 signaling decreases CRBN expression, reducing responses to lenalidomide. RSK2 or CREB1 inhibition increased CRBN levels and were synergic with lenalidomide in inducing cell death, especially in CD56-expressing multiple myeloma cells. In conclusion, our findings demonstrate that CD56 promotes multiple myeloma cell growth, and pave the way to novel therapies based on targeting CD56, along with the use of CD56 as a predictive biomarker for multiple myeloma therapies.
Implications:
Multiple myeloma is an incurable, genetically heterogeneous disease, without available tailored therapeutic approaches. CD56 signaling promotes multiple myeloma growth and adhesion, by activating CREB1 target genes, MCL1 and BCL2. Inhibition of CREB1 alone or in combination with lenalidomide is an unexplored synthetic lethal approach in CD56-expressing patients with multiple myeloma.
Data from Inhibition of LSD1 Attenuates Oral Cancer Development and Promotes Therapeutic Efficacy of Immune Checkpoint Blockade and YAP/TAZ Inhibition
Authors: Alhousami Thabet , Diny Michael , Ali Faiza , Shin Jennifer , Kumar Gaurav , Kumar Vikas , Campbell Joshua D. , Noonan Vikki , Hanna Glenn J. , Denis Gerald V. , Monti Stefano , Kukuruzinska Maria A. , Varelas Xaralabos , Bais Manish V.
Published: April 2023

Abstract
Lysine-specific demethylase 1 (LSD1) is a histone demethylase that contributes to the etiology of oral squamous cell carcinoma (OSCC) in part by promoting cancer stem cell phenotypes. The molecular signals regulated by LSD1, or acting with LSD1, are poorly understood, particularly in the development of OSSC. In this study, we show that conditional deletion of the Lsd1 gene or pharmacologic inhibition of LSD1 in the tongue epithelium leads to reduced development of OSCC following exposure to the tobacco carcinogen 4NQO. LSD1 inhibition attenuated proliferation and clonogenic survival and showed an additive effect when combined with the YAP inhibitor Verteporfin. Interestingly, LSD1 inhibition upregulated the expression of PD-L1, leading to immune checkpoint inhibitor therapy responses.
Implications:
Collectively, our studies reveal a critical role for LSD1 in OSCC development and identification of tumor growth targeting strategies that can be combined with LSD1 inhibition for improved therapeutic application.
Data from Disruption of β-Catenin–Dependent Wnt Signaling in Colon Cancer Cells Remodels the Microenvironment to Promote Tumor Invasion
Authors: Chen George T. , Tifrea Delia F. , Murad Rabi , Habowski Amber N. , Lyou Yung , Duong Madeleine R. , Hosohama Linzi , Mortazavi Ali , Edwards Robert A. , Waterman Marian L.
Published: April 2023

Abstract
The recent classification of colon cancer into molecular subtypes revealed that patients with the poorest prognosis harbor tumors with the lowest levels of Wnt signaling. This is contrary to the general understanding that overactive Wnt signaling promotes tumor progression from early initiation stages through to the later stages including invasion and metastasis. Here, we directly test this assumption by reducing the activity of ß-catenin–dependent Wnt signaling in colon cancer cell lines at either an upstream or downstream step in the pathway. We determine that Wnt-reduced cancer cells exhibit a more aggressive disease phenotype, including increased mobility in vitro and disruptive invasion into mucosa and smooth muscle in an orthotopic mouse model. RNA sequencing reveals that interference with Wnt signaling leads to an upregulation of gene programs that favor cell migration and invasion and a downregulation of inflammation signatures in the tumor microenvironment. We identify a set of upregulated genes common among the Wnt perturbations that are predictive of poor patient outcomes in early-invasive colon cancer. Our findings suggest that while targeting Wnt signaling may reduce tumor burden, an inadvertent side effect is the emergence of invasive cancer.
Implications:
Decreased Wnt signaling in colon tumors leads to a more aggressive disease phenotype due to an upregulation of gene programs favoring cell migration in the tumor and downregulation of inflammation programs in the tumor microenvironment; these impacts must be carefully considered in developing Wnt-targeting therapies.

Watch the interview with Marian L. Waterman, PhD, recipient of the 2023 MCR Michael B. Kastan Award for Research Excellence: https://vimeo.com/847435577
Supplementary Figure 2 from Effects of Ginger Supplementation on Cell-Cycle Biomarkers in the Normal-Appearing Colonic Mucosa of Patients at Increased Risk for Colorectal Cancer: Results from a Pilot, Randomized, and Controlled Trial
Authors: Citronberg Jessica , Bostick Roberd , Ahearn Thomas , Turgeon D. Kim , Ruffin Mack T. , Djuric Zora , Sen Ananda , Brenner Dean E. , Zick Suzanna M.
Published: April 2023

PDF file - 95K, Flow Diagram of a Trial of Ginger Supplementation Over Four Weeks on Markers of Apoptosis, Proliferation and Differentiation in the Normal-Appearing Colorectal Mucosa of Individuals at Increased Risk of CRC
Supplementary Methods, Table 1, Figure Legends 1-2 from Effect of Orally Administered Bovine Lactoferrin on the Growth of Adenomatous Colorectal Polyps in a Randomized, Placebo-Controlled Clinical Trial
Authors: Kozu Takahiro , Iinuma Gen , Ohashi Yasuo , Saito Yutaka , Akasu Takayuki , Saito Daizo , Alexander David B. , Iigo Masaaki , Kakizoe Tadao , Tsuda Hiroyuki
Published: April 2023

Supplementary Methods, Table 1, Figure Legends 1-2 from Effect of Orally Administered Bovine Lactoferrin on the Growth of Adenomatous Colorectal Polyps in a Randomized, Placebo-Controlled Clinical Trial
Related Perspective from A Genome-Wide Association Study Identifies a Locus on Chromosome 14q21 as a Predictor of Leukocyte Telomere Length and as a Marker of Susceptibility for Bladder Cancer
Authors: Gu Jian , Chen Meng , Shete Sanjay , Amos Christopher I. , Kamat Ashish , Ye Yuanqing , Lin Jie , Dinney Colin P. , Wu Xifeng
Published: April 2023

Related Perspective from A Genome-Wide Association Study Identifies a Locus on Chromosome 14q21 as a Predictor of Leukocyte Telomere Length and as a Marker of Susceptibility for Bladder Cancer
Supplementary Table 1 from Chemopreventive Doses of Methylselenocysteine Alter Circadian Rhythm in Rat Mammary Tissue
Authors: Zhang Xun , Zarbl Helmut
Published: April 2023

Supplementary Table 1 from Chemopreventive Doses of Methylselenocysteine Alter Circadian Rhythm in Rat Mammary Tissue
Supplementary Table 2 from The Chemopreventive Effect of Mifepristone on Mammary Tumorigenesis Is Associated with an Anti-invasive and Anti-inflammatory Gene Signature
Authors: Yuan Hongyan , Upadhyay Geeta , Lu Jin , Kopelovich Levy , Glazer Robert I.
Published: April 2023

XLS file - 96K, Gene array data form wild-type...
Supplementary Figure 2 from The Chemopreventive Effect of Mifepristone on Mammary Tumorigenesis Is Associated with an Anti-invasive and Anti-inflammatory Gene Signature
Authors: Yuan Hongyan , Upadhyay Geeta , Lu Jin , Kopelovich Levy , Glazer Robert I.
Published: April 2023

PDF file - 115K, Heatmap
Related Article from The Monkey, the Hen, and the Mouse: Models to Advance Ovarian Cancer Chemoprevention
Authors: Lu Karen H. , Yates Melinda S. , Mok Samuel C.
Published: April 2023

Related Article from The Monkey, the Hen, and the Mouse: Models to Advance Ovarian Cancer Chemoprevention
Related Perspective from Physical Activity and Survival in Postmenopausal Women with Breast Cancer: Results from the Women's Health Initiative
Authors: Irwin Melinda L. , McTiernan Anne , Manson JoAnn E. , Thomson Cynthia A. , Sternfeld Barbara , Stefanick Marcia L. , Wactawski-Wende Jean , Craft Lynette , Lane Dorothy , Martin Lisa W. , Chlebowski Rowan
Published: April 2023

Related Perspective from Physical Activity and Survival in Postmenopausal Women with Breast Cancer: Results from the Women's Health Initiative
Supplementary Table 1 from Novel Susceptibility Loci for Second Primary Tumors/Recurrence in Head and Neck Cancer Patients: Large-Scale Evaluation of Genetic Variants
Authors: Wu Xifeng , Spitz Margaret R. , Lee J. Jack , Lippman Scott M. , Ye Yuanqing , Yang Hushan , Khuri Fadlo R. , Kim Edward , Gu Jian , Lotan Reuben , Hong Waun K.
Published: April 2023

Supplementary Table 1 from Novel Susceptibility Loci for Second Primary Tumors/Recurrence in Head and Neck Cancer Patients: Large-Scale Evaluation of Genetic Variants
Perspective on this Article from Novel Susceptibility Loci for Second Primary Tumors/Recurrence in Head and Neck Cancer Patients: Large-Scale Evaluation of Genetic Variants
Authors: Wu Xifeng , Spitz Margaret R. , Lee J. Jack , Lippman Scott M. , Ye Yuanqing , Yang Hushan , Khuri Fadlo R. , Kim Edward , Gu Jian , Lotan Reuben , Hong Waun K.
Published: April 2023

Perspective on this Article from Novel Susceptibility Loci for Second Primary Tumors/Recurrence in Head and Neck Cancer Patients: Large-Scale Evaluation of Genetic Variants
Supplementary Table 9 from Characterizing the Impact of Smoking and Lung Cancer on the Airway Transcriptome Using RNA-Seq
Authors: Beane Jennifer , Vick Jessica , Schembri Frank , Anderlind Christina , Gower Adam , Campbell Joshua , Luo Lingqi , Zhang Xiao Hui , Xiao Ji , Alekseyev Yuriy O. , Wang Shenglong , Levy Shawn , Massion Pierre P. , Lenburg Marc , Spira Avrum
Published: April 2023

Supplementary Table 9 from Characterizing the Impact of Smoking and Lung Cancer on the Airway Transcriptome Using RNA-Seq
Supplementary Figures 1-2 from Immunomodulation of Curcumin on Adoptive Therapy with T Cell Functional Imaging in Mice
Authors: Chang Ya-Fang , Chuang Hui-Yen , Hsu Chien-Hui , Liu Ren-Shyan , Gambhir Sanjiv Sam , Hwang Jeng-Jong
Published: April 2023

PDF file - 160K
Supplementary Figure 4 from Dietary Resveratrol Prevents Development of High-Grade Prostatic Intraepithelial Neoplastic Lesions: Involvement of SIRT1/S6K Axis
Authors: Li Guiming , Rivas Paul , Bedolla Roble , Thapa Dinesh , Reddick Robert L. , Ghosh Rita , Kumar Addanki P.
Published: April 2023

PDF file - 94KB, Effect of RES on body weight and food consumption in PTEN knockout mice
Supplementary Tables 1-2 from The Core Circadian Gene Cryptochrome 2 Influences Breast Cancer Risk, Possibly by Mediating Hormone Signaling
Authors: Hoffman Aaron E. , Zheng Tongzhang , Yi Chun-Hui , Stevens Richard G. , Ba Yue , Zhang Yawei , Leaderer Derek , Holford Theodore , Hansen Johnni , Zhu Yong
Published: April 2023

Supplementary Tables 1-2 from The Core Circadian Gene Cryptochrome 2 Influences Breast Cancer Risk, Possibly by Mediating Hormone Signaling
Appendix from Health Benefits and Cost-Effectiveness of Primary Genetic Screening for Lynch Syndrome in the General Population
Authors: Dinh Tuan A. , Rosner Benjamin I. , Atwood James C. , Boland C. Richard , Syngal Sapna , Vasen Hans F. A. , Gruber Stephen B. , Burt Randall W.
Published: April 2023

Appendix from Health Benefits and Cost-Effectiveness of Primary Genetic Screening for Lynch Syndrome in the General Population
Supplementary Tables 1-5 from Biomarkers of Dietary Energy Restriction in Women at Increased Risk of Breast Cancer
Authors: Ong Kai Ren , Sims Andrew H. , Harvie Michelle , Chapman Mary , Dunn Warwick B. , Broadhurst David , Goodacre Royston , Wilson Mary , Thomas Nicola , Clarke Robert B. , Howell Anthony
Published: April 2023

Supplementary Tables 1-5 from Biomarkers of Dietary Energy Restriction in Women at Increased Risk of Breast Cancer
Supplementary Figure 1 from A Hexane Fraction of American Ginseng Suppresses Mouse Colitis and Associated Colon Cancer: Anti-inflammatory and Proapoptotic Mechanisms
Authors: Poudyal Deepak , Mai Le Phuong , Davis Tia , Hofseth Anne B. , Chumanevich Alena , Chumanevich Alexander A. , Wargovich Michael J. , Nagarkatti Mitzi , Nagarkatti Prakash S. , Windust Anthony , Hofseth Lorne J.
Published: April 2023

PDF file - 814K, Experimental protocol for the DSS mouse model of Ulcerative Colitis. 11.9 mg/kg/day of whole AG extract, the Hexane Fraction of AG or Vehicle (1x PBS) was given to the respective group of mice by oral gavage after 1.5 cycles of DSS and continued daily until the end of the experiment (5.5 cycles). The mice from each group were euthanized on days indicated.
Supplementary Table 1 from The Chemopreventive Efficacies of Nonsteroidal Anti-inflammatory Drugs: The Relationship of Short-term Biomarkers to Long-term Skin Tumor Outcome
Authors: Mikulec Carol D. , Rundhaug Joyce E. , Simper Melissa S. , Lubet Ronald A. , Fischer Susan M.
Published: April 2023

Supplementary Table 1 - PDF file 50K, Effect of NSAIDs on UV-induced Epidermal Thickness and Apoptosis
Supplementary Figure 2 from Deficiency in the 15-kDa Selenoprotein Inhibits Tumorigenicity and Metastasis of Colon Cancer Cells
Authors: Irons Robert , Tsuji Petra A. , Carlson Bradley A. , Ouyang Ping , Yoo Min-Hyuk , Xu Xue-Ming , Hatfield Dolph L. , Gladyshev Vadim N. , Davis Cindy D.
Published: April 2023

Supplementary Figure 2 from Deficiency in the 15-kDa Selenoprotein Inhibits Tumorigenicity and Metastasis of Colon Cancer Cells
2015.02.16_Supplementary Figures_OPL methylation manuscript from New DNA Methylation Markers and Global DNA Hypomethylation Are Associated with Oral Cancer Development
Authors: Foy Jean-Philippe , Pickering Curtis R. , Papadimitrakopoulou Vassiliki A. , Jelinek Jaroslav , Lin Steven H. , William William N. , Frederick Mitchell J. , Wang Jing , Lang Wenhua , Feng Lei , Zhang Li , Kim Edward S. , Fan You H. , Hong Waun K. , El-Naggar Adel K. , Lee J. Jack , Myers Jeffrey N. , Issa Jean-Pierre , Lippman Scott M. , Mao Li , Saintigny Pierre
Published: April 2023

Supplementary Figure 1: Flow-chart describing the selection of 86 candidate genes. Supplementary Figure 2: Promoter methylation of 62/86 candidate genes in head and neck cancer, normal mucosa and blood. Supplementary Figure 3: Promoter methylation of 13/86 candidate genes in normal tissues. Supplementary Fig. 4: Hierarchical clustering of paired normal, dysplastic lesions and carcinomas (in situ or invasive) collected from 10 patients (3), using methylation profiles of 60/86 of our candidate genes. Supplementary Figure 5: (A) Hierarchical cluster analysis of oral squamous cell carcinoma and oral normal mucosa using candidate genes expression profiles. Supplementary Figure 6: Correlation of the percentage methylation of AGTR1.
Supplementary Materials 3 from Characterizing the Molecular Spatial and Temporal Field of Injury in Early-Stage Smoker Non–Small Cell Lung Cancer Patients after Definitive Surgery by Expression Profiling
Authors: Kadara Humam , Shen Li , Fujimoto Junya , Saintigny Pierre , Chow Chi-Wan , Lang Wenhua , Chu Zuoming , Garcia Melinda , Kabbout Mohamed , Fan You-Hong , Behrens Carmen , Liu Diane A. , Mao Li , Lee J. Jack , Gold Kathryn A. , Wang Jing , Coombes Kevin R. , Kim Edward S. , Hong Waun Ki , Wistuba Ignacio I.
Published: April 2023

PDF file - 129K, Sweave report depicting complete codes use for microarray analysis of paired adjacent and contralateral airways
Supplementary Materials 2 from Characterizing the Molecular Spatial and Temporal Field of Injury in Early-Stage Smoker Non–Small Cell Lung Cancer Patients after Definitive Surgery by Expression Profiling
Authors: Kadara Humam , Shen Li , Fujimoto Junya , Saintigny Pierre , Chow Chi-Wan , Lang Wenhua , Chu Zuoming , Garcia Melinda , Kabbout Mohamed , Fan You-Hong , Behrens Carmen , Liu Diane A. , Mao Li , Lee J. Jack , Gold Kathryn A. , Wang Jing , Coombes Kevin R. , Kim Edward S. , Hong Waun Ki , Wistuba Ignacio I.
Published: April 2023

PDF file - 6272K, Sweave report depicting complete codes used for microarray analysis and identification of genes differentially expressed by site and time
Supplementary Table 1, Figure 1 from Low Integrated DNA Repair Score and Lung Cancer Risk
Authors: Sevilya Ziv , Leitner-Dagan Yael , Pinchev Mila , Kremer Ran , Elinger Dalia , Rennert Hedy S. , Schechtman Edna , Freedman Laurence S. , Rennert Gad , Paz-Elizur Tamar , Livneh Zvi
Published: April 2023

PDF file - 856K, Supplementary Table 1, presenting Conditional logistic regression model relating the three biomarkers OGG1, MPG and APE1 to lung cancer. Supplementary Figure 1, presenting Association of DNA repair activity with lung cancer risk, including relative frequency plots for APE1 activity in 100 case patients and 99 matched controls subjects, relative frequency plots for the integrated DNA repair OMA score in 100 case patients and 99 matched controls subjects, and odds ratio calculated as a function of the percentiles of the distribution of the OMA score among the control subjects.
Supplementary Figures 1 - 3 from Characterizing the Molecular Spatial and Temporal Field of Injury in Early-Stage Smoker Non–Small Cell Lung Cancer Patients after Definitive Surgery by Expression Profiling
Authors: Kadara Humam , Shen Li , Fujimoto Junya , Saintigny Pierre , Chow Chi-Wan , Lang Wenhua , Chu Zuoming , Garcia Melinda , Kabbout Mohamed , Fan You-Hong , Behrens Carmen , Liu Diane A. , Mao Li , Lee J. Jack , Gold Kathryn A. , Wang Jing , Coombes Kevin R. , Kim Edward S. , Hong Waun Ki , Wistuba Ignacio I.
Published: April 2023

PDF file - 125K, Histograms of the p-values for site- and time-dependent differential expression; Site-dependent differential gene expression patterns identified by expression profiling of airways excluding main carinas; Smoothened scatter plot of transformed p-values for site and time effects
Perspective on This Article from Neonatal Experiences Differentially Influence Mammary Gland Morphology, Estrogen Receptor α Protein Levels, and Carcinogenesis in BALB/c Mice
Authors: Boyd Allison L. , Salleh Ayesha , Humber Brent , Yee Janet , Tomes Ladislav , Kerr Leslie R.
Published: April 2023

Perspective on This Article from Neonatal Experiences Differentially Influence Mammary Gland Morphology, Estrogen Receptor α Protein Levels, and Carcinogenesis in BALB/c Mice
Supplementary Figures 1 - 2, Tables 1 - 2 from Molecular Profiling of Premalignant Lesions in Lung Squamous Cell Carcinomas Identifies Mechanisms Involved in Stepwise Carcinogenesis
Authors: Ooi Aik T. , Gower Adam C. , Zhang Kelvin X. , Vick Jessica L. , Hong Longsheng , Nagao Brian , Wallace W. Dean , Elashoff David A. , Walser Tonya C. , Dubinett Steven M. , Pellegrini Matteo , Lenburg Marc E. , Spira Avrum , Gomperts Brigitte N.
Published: April 2023

PDF file - 690KB, Supplementary Figure S1. Laser capture microdissection. Supplementary Figure S2. Experimental validation of PTBP3 expression. Supplementary Table S1. Patient demographics. List of SCC patients with their age, gender, grade of premalignant lesion, and assays performed. Supplementary Table S2. Alignment statistics. The number and percentage of reads aligning uniquely to the nuclear (autosomal and sex) or mitochondrial chromosomes, or not uniquely aligned, are shown for each sample.
Supplementary Tables 1 - 2 from Emergence of HPV16-Positive Oropharyngeal Cancer in Black Patients Over Time: University of Maryland 1992–2007
Authors: Zandberg Dan P. , Liu Sandy , Goloubeva Olga G. , Schumaker Lisa M. , Cullen Kevin J.
Published: April 2023

PDF file - 40K, Supplemental Table 1. Selected Demographic and Baseline Characteristics of the Patients. Supplemental Table 2. Selected Demographic and Baseline Characteristics of the HPV16 positive.
Supplementary Table S2 from Effects of Metformin, Buformin, and Phenformin on the Post-Initiation Stage of Chemically Induced Mammary Carcinogenesis in the Rat
Authors: Zhu Zongjian , Jiang Weiqin , Thompson Matthew D. , Echeverria Dimas , McGinley John N. , Thompson Henry J.
Published: April 2023

Supplementary Table S2. Plasma and tissue concentrations of biguanides
Supplementary Figure S2 from Effects of Metformin, Buformin, and Phenformin on the Post-Initiation Stage of Chemically Induced Mammary Carcinogenesis in the Rat
Authors: Zhu Zongjian , Jiang Weiqin , Thompson Matthew D. , Echeverria Dimas , McGinley John N. , Thompson Henry J.
Published: April 2023

Supplementary Figure S2. Representative composite gels for Western blotting of mammary carcinomas
Supplementary Table 5 from Prediction of Recurrence and Survival in Hepatocellular Carcinoma Based on Two Cox Models Mainly Determined by FoxP3+ Regulatory T Cells
Authors: Lin Sheng-Zhang , Chen Kang-Jie , Xu Zhi-Yuan , Chen Hui , Zhou Lin , Xie Hai-Yang , Zheng Shu-Sen
Published: April 2023

PDF file - 73K, Correlation of FoxP3+/CD4+, IL-17+/CD3+ and Trypase+ cells with Clinicopathologic characteristics.
Supplemental Tables 1-5 from Omega-3 and Omega-6 Fatty Acids in Blood and Breast Tissue of High-Risk Women and Association with Atypical Cytomorphology
Authors: Hidaka Brandon H. , Li Shengqi , Harvey Katherine E. , Carlson Susan E. , Sullivan Debra K. , Kimler Bruce F. , Zalles Carola M. , Fabian Carol J.
Published: April 2023

Supplemental Tables 1-5. Supplemental Table 1: comparison of fatty acid composition by cytologic atypia or not. Supplemental Table 2: Comparison of fatty acid composition of plasma phospholipids (PLs) by evidence of cytology atypia. Supplemental Table 3: Comparison of fatty acid composition of plasma triacylglycerides (TAGs) by evidence of cytology atypia. Supplemental Table 4: Comparison of fatty acid composition of breast phospholipids (PLs) by evidence of cytology atypia. Supplemental Table 5: Comparison of fatty acid composition of breast triacylglycerides (TAGs) by evidence of cytology atypia.
Supplementary Tables 1-6 from Blood Biomarker Levels to Aid Discovery of Cancer-Related Single-Nucleotide Polymorphisms: Kallikreins and Prostate Cancer
Authors: Klein Robert J. , Halldén Christer , Cronin Angel M. , Ploner Alexander , Wiklund Fredrik , Bjartell Anders S. , Stattin Pär , Xu Jianfeng , Scardino Peter T. , Offit Kenneth , Vickers Andrew J. , Grönberg Henrik , Lilja Hans
Published: April 2023

Supplementary Tables 1-6 from Blood Biomarker Levels to Aid Discovery of Cancer-Related Single-Nucleotide Polymorphisms: Kallikreins and Prostate Cancer
Supplementary Figure Legend from A Novel Prodrug of Epigallocatechin-3-gallate: Differential Epigenetic hTERT Repression in Human Breast Cancer Cells
Authors: Meeran Syed M. , Patel Shweta N. , Chan Tak-Hang , Tollefsbol Trygve O.
Published: April 2023

PDF file - 51K
Supplementary Figure 1 from STAT2 Contributes to Promotion of Colorectal and Skin Carcinogenesis
Authors: Gamero Ana M. , Young Matthew R. , Mentor-Marcel Roycelynn , Bobe Gerd , Scarzello Anthony J. , Wise Jennifer , Colburn Nancy H.
Published: April 2023

Supplementary Figure 1 from STAT2 Contributes to Promotion of Colorectal and Skin Carcinogenesis
Supplementary Figures 1 - 3 from Licochalcone E Present in Licorice Suppresses Lung Metastasis in the 4T1 Mammary Orthotopic Cancer Model
Authors: Kwon Soo Jin , Park So Young , Kwon Gyoo Taik , Lee Ki Won , Kang Young-Hee , Choi Myung-Sook , Yun Jong Won , Jeon Jae-Ho , Jun Jong Gab , Park Jung Han Yoon
Published: April 2023

PDF file - 303K, Supplemental Figure 1. Chemical structure of licochalcone E (LicE). Supplemental Figure 2. Licochalcone E decreases the tube formation of endothelial cells. Supplemental Figure 3. Licochalcone E decreases the secretion of urokinase-type plasminogen activator (uPA), VEGF-A, and MMP-9 in MDA-MB-231 breast cancer cells.
Supplementary Figures from NID2 and HOXA9 Promoter Hypermethylation as Biomarkers for Prevention and Early Detection in Oral Cavity Squamous Cell Carcinoma Tissues and Saliva
Authors: Guerrero-Preston R. , Soudry E. , Acero J. , Orera M. , Moreno-López L. , Macía-Colón G. , Jaffe A. , Berdasco M. , Ili-Gangas C. , Brebi-Mieville P. , Fu Y. , Engstrom C. , Irizarry R.A. , Esteller M. , Westra W. , Koch W. , Califano J. , Sidransky D.
Published: April 2023

Supplementary Figures from NID2 and HOXA9 Promoter Hypermethylation as Biomarkers for Prevention and Early Detection in Oral Cavity Squamous Cell Carcinoma Tissues and Saliva
Supplementary Table 2 from Prostatic and Dietary Omega-3 Fatty Acids and Prostate Cancer Progression during Active Surveillance
Authors: Moreel Xavier , Allaire Janie , Léger Caroline , Caron André , Labonté Marie-Ève , Lamarche Benoît , Julien Pierre , Desmeules Patrice , Têtu Bernard , Fradet Vincent
Published: April 2023

PDF file - 76K, Supplementary Table S2. Mean of calculated coefficient of variation between duplicate prostate biopsies determined for specific fatty acids.
Perspective on this Article from Biological Activity of Celecoxib in the Bronchial Epithelium of Current and Former Smokers
Authors: Kim Edward S. , Hong Waun K. , Lee J. Jack , Mao Li , Morice Rodolfo C. , Liu Diane D. , Jimenez Carlos A. , Eapen Georgie A. , Lotan Reuben , Tang Ximing , Newman Robert A. , Wistuba Ignacio I. , Kurie Jonathan M.
Published: April 2023

Perspective on this Article from Biological Activity of Celecoxib in the Bronchial Epithelium of Current and Former Smokers
Supplementary Table 2 from Lycopene Metabolite, Apo-10′-Lycopenoic Acid, Inhibits Diethylnitrosamine-Initiated, High Fat Diet–Promoted Hepatic Inflammation and Tumorigenesis in Mice
Authors: Ip Blanche C. , Hu Kang-Quan , Liu Chun , Smith Donald E. , Obin Martin S. , Ausman Lynne M. , Wang Xiang-Dong
Published: April 2023

PDF file - 44K, Supplementary Table S2. Primer sequences for RT-PCR.
Related Article from Aspirin and Familial Adenomatous Polyposis: Coming Full Circle
Authors: Chan Andrew T.
Published: April 2023

Related Article from Aspirin and Familial Adenomatous Polyposis: Coming Full Circle
Supplementary Figure 4 from Breast Cancer Cell Apoptosis with Phytoestrogens Is Dependent on an Estrogen-Deprived State
Authors: Obiorah Ifeyinwa E. , Fan Ping , Jordan V. Craig
Published: April 2023

PDF file - 41K, Anti-estrogen, ICI 182 780 blocks phytoestrogen inhibited growth MCF7:5C cells were treated with E2(1nM), equilin (1nM), phytoestrogens (1microM) in presence of various concentrations of ICI 182 780. Total DNA was assessed in each well using a fluorescent DNA quantification kit.
Supplementary Table 1 from Class I HDACs Are Mediators of Smoke Carcinogen–Induced Stabilization of DNMT1 and Serve as Promising Targets for Chemoprevention of Lung Cancer
Authors: Brodie Seth A. , Li Ge , El-Kommos Adam , Kang Hyunseok , Ramalingam Suresh S. , Behera Madhusmita , Gandhi Khanjan , Kowalski Jeanne , Sica Gabriel L. , Khuri Fadlo R. , Vertino Paula M. , Brandes Johann C.
Published: April 2023

PDF file - 47K, Differentially methylated genes with beta values (3KT vs carcinogen exposed).
Supplementary Figure 2 from A DRD1 Polymorphism Predisposes to Lung Cancer among Those Exposed to Secondhand Smoke during Childhood
Authors: Robles Ana I. , Yang Ping , Jen Jin , McClary Andrew C. , Calhoun Kara , Bowman Elise D. , Vähäkangas Kirsi , Greathouse K. Leigh , Wang Yi , Olivo-Marston Susan , Wenzlaff Angela S. , Deng Bo , Schwartz Ann G. , Ryan Bríd M.
Published: April 2023

Relationship between rs686 and pack-years of smoking (A) in the NCI-MD (B) and Wayne State (C) studies. Relationship between rs686 and age at smoking initiation (D) in the NCI-MD study.
Supplementary Figure 1 from Licochalcone A, a Natural Inhibitor of c-Jun N-Terminal Kinase 1
Authors: Yao Ke , Chen Hanyong , Lee Mee-Hyun , Li Haitao , Ma Weiya , Peng Cong , Song Nu Ry , Lee Ki Won , Bode Ann M. , Dong Ziming , Dong Zigang
Published: April 2023

PDF file 58K, Figure S1. (A) Licochalcone A does not bind with p38 or ERKs. Whole lysates from JNKwt MEFs were incubated with 30 microL of licochalcone A-Sepharose 4B beads (50% slurry) for 2 h at 4{degree sign}C. Beads were washed and p38 or ERKs binding was visualized by Western blotting. (B) Truncated JNK1 fragments bind with licochalcone A. The indicated JNK1 expression vector was transiently introduced into 293 cells. Total cell lysates (200 microg) were used for licochalcone A Sepharose 4B binding and bound proteins were visualized by Western blotting with anti-GAL4
Supplementary Figure 2 from DACT2 Is a Candidate Tumor Suppressor and Prognostic Marker in Esophageal Squamous Cell Carcinoma
Authors: Hou Jian , Liao Lian-Di , Xie Yang-Min , Zeng Fa-Min , Ji Xia , Chen Bo , Li Li-Yan , Zhu Meng-Xiao , Yang Cui-Xia , Chen Tao , Xu Xiu-E , Shen Jian , Guo Ming-Zhou , Li En-Min , Xu Li-Yan
Published: April 2023

PDF file - 111K, DACT2-mediated suppression of tumorigenicity of ESCC cells in vivo.