bluuebunny/crossref_metadata_2025 huggingface dataset:
Secondary Structural Model of Human MALAT1 Reveals Multiple Structure–Function Relationships
Authors: McCown Phillip J. , Wang Matthew C. , Jaeger Luc , Brown Jessica A.
Published: November 2019

Human metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) is an abundant nuclear-localized long noncoding RNA (lncRNA) that has significant roles in cancer. While the interacting partners and evolutionary sequence conservation of MALAT1 have been examined, much of the structure of MALAT1 is unknown. Here, we propose a hypothetical secondary structural model for 8425 nucleotides of human MALAT1 using three experimental datasets that probed RNA structures in vitro and in various human cell lines. Our model indicates that approximately half of human MALAT1 is structured, forming 194 helices, 13 pseudoknots, five structured tetraloops, nine structured internal loops, and 13 intramolecular long-range interactions that give rise to several multiway junctions. Evolutionary conservation and covariation analyses support 153 of 194 helices in 51 mammalian MALAT1 homologs and 42 of 194 helices in 53 vertebrate MALAT1 homologs, thereby identifying an evolutionarily conserved core that likely has important functional roles in mammals and vertebrates. Data mining revealed that RNA modifications, somatic cancer-associated mutations, and single-nucleotide polymorphisms may induce structural rearrangements that sequester or expose binding sites for several cancer-associated microRNAs. Our findings reveal new mechanistic leads into the roles of MALAT1 by identifying several intriguing structure–function relationships in which the dynamic structure of MALAT1 underlies its biological functions.
The Pathogenesis of Endometriosis: Molecular and Cell Biology Insights
Authors: Laganà Antonio Simone , Garzon Simone , Götte Martin , Viganò Paola , Franchi Massimo , Ghezzi Fabio , Martin Dan C.
Published: November 2019

The etiopathogenesis of endometriosis is a multifactorial process resulting in a heterogeneous disease. Considering that endometriosis etiology and pathogenesis are still far from being fully elucidated, the current review aims to offer a comprehensive summary of the available evidence. We performed a narrative review synthesizing the findings of the English literature retrieved from computerized databases from inception to June 2019, using the Medical Subject Headings (MeSH) unique ID term “Endometriosis” (ID:D004715) with “Etiology” (ID:Q000209), “Immunology” (ID:Q000276), “Genetics” (ID:D005823) and “Epigenesis, Genetic” (ID:D044127). Endometriosis may origin from Müllerian or non-Müllerian stem cells including those from the endometrial basal layer, Müllerian remnants, bone marrow, or the peritoneum. The innate ability of endometrial stem cells to regenerate cyclically seems to play a key role, as well as the dysregulated hormonal pathways. The presence of such cells in the peritoneal cavity and what leads to the development of endometriosis is a complex process with a large number of interconnected factors, potentially both inherited and acquired. Genetic predisposition is complex and related to the combined action of several genes with limited influence. The epigenetic mechanisms control many of the processes involved in the immunologic, immunohistochemical, histological, and biological aberrations that characterize the eutopic and ectopic endometrium in affected patients. However, what triggers such alterations is not clear and may be both genetically and epigenetically inherited, or it may be acquired by the particular combination of several elements such as the persistent peritoneal menstrual reflux as well as exogenous factors. The heterogeneity of endometriosis and the different contexts in which it develops suggest that a single etiopathogenetic model is not sufficient to explain its complex pathobiology.
SH3Ps—Evolution and Diversity of a Family of Proteins Engaged in Plant Cytokinesis
Authors: Baquero Forero Anežka , Cvrčková Fatima
Published: November 2019

SH3P2 (At4g34660), an Arabidopsis thaliana SH3 and Bin/amphiphysin/Rvs (BAR) domain-containing protein, was reported to have a specific role in cell plate assembly, unlike its paralogs SH3P1 (At1g31440) and SH3P3 (At4g18060). SH3P family members were also predicted to interact with formins—evolutionarily conserved actin nucleators that participate in microtubule organization and in membrane–cytoskeleton interactions. To trace the origin of functional specialization of plant SH3Ps, we performed phylogenetic analysis of SH3P sequences from selected plant lineages. SH3Ps are present in charophytes, liverworts, mosses, lycophytes, gymnosperms, and angiosperms, but not in volvocal algae, suggesting association of these proteins with phragmoplast-, but not phycoplast-based cell division. Separation of three SH3P clades, represented by SH3P1, SH3P2, and SH3P3 of A. thaliana, appears to be a seed plant synapomorphy. In the yeast two hybrid system, Arabidopsis SH3P3, but not SH3P2, binds the FH1 and FH2 domains of the formin FH5 (At5g54650), known to participate in cytokinesis, while an opposite binding specificity was found for the dynamin homolog DRP1A (At5g42080), confirming earlier findings. This suggests that the cytokinetic role of SH3P2 is not due to its interaction with FH5. Possible determinants of interaction specificity of SH3P2 and SH3P3 were identified bioinformatically.
Staphylococcus aureus Nasal Carriage and Autoimmune Diseases: From Pathogenic Mechanisms to Disease Susceptibility and Phenotype
Authors: Ceccarelli Fulvia , Perricone Carlo , Olivieri Giulio , Cipriano Enrica , Spinelli Francesca , Valesini Guido , Conti Fabrizio
Published: November 2019

The role of infective agents in autoimmune diseases (ADs) development has been historically investigated, but in the last years has been strongly reconsidered due to the interest in the link between the microbiome and ADs. Together with the gut, the skin microbiome is characterized by the presence of several microorganisms, potentially influencing innate and adaptive immune response. S. aureus is one of the most important components of the skin microbiome that can colonize anterior nares without clinical manifestations. Data from the literature demonstrates a significantly higher prevalence of nasal colonization in ADs patients in comparison with healthy subjects, suggesting a possible role in terms of disease development and phenotypes. Thus, in the present narrative review we focused on the mechanisms by which S. aureus could influence the immune response and on its relationship with ADs, in particular granulomatosis with polyangiitis, rheumatoid arthritis, and systemic lupus erythematosus.
Micronutrient Depletion in Heart Failure: Common, Clinically Relevant and Treatable
Authors: Cvetinovic Natasa , Loncar Goran , Isakovic Andjelka M. , von Haehling Stephan , Doehner Wolfram , Lainscak Mitja , Farkas Jerneja
Published: November 2019

Heart failure (HF) is a chronic condition with many imbalances, including nutritional issues. Next to sarcopenia and cachexia which are clinically evident, micronutrient deficiency is also present in HF. It is involved in HF pathophysiology and has prognostic implications. In general, most widely known micronutrients are depleted in HF, which is associated with symptoms and adverse outcomes. Nutritional intake is important but is not the only factor reducing the micronutrient availability for bodily processes, because absorption, distribution, and patient comorbidity may play a major role. In this context, interventional studies with parenteral micronutrient supplementation provide evidence that normalization of micronutrients is associated with improvement in physical performance and quality of life. Outcome studies are underway and should be reported in the following years.
Anti-Inflammatory and Anti-Hyperuricemic Functions of Two Synthetic Hybrid Drugs with Dual Biological Active Sites
Authors: Almeer Rafa S. , Hammad Sherif F. , Leheta Ola F. , Abdel Moneim Ahmed E. , Amin Hatem K.
Published: November 2019

The present study aimed to test the anti-inflammatory and xanthine oxidase inhibitory activities of two synthesized molecules and compare them to routinely prescribed nonsteroidal anti-inflammatory drugs (NSAIDs), such as diclofenac and the serum urate-lowering drug, allopurinol. The anti-inflammatory effects of the designed compounds (A and B) were evaluated in carrageenan (CAR)-induced paw edema in mice. The levels of nitric oxide and myeloperoxidase activity were measured in paw skin using biochemical methods. Additionally, prostaglandin E2 (PGE2), C-reactive protein (CRP), cyclooxygenase-2 (Cox-2), tumor necrosis factor-α (TNFα), interleukin (IL)-1β, IL-2 and IL-10, and monocyte chemoattractant protein-1 (MCP1) were assessed by enzyme-linked immunosorbent assay (ELISA). The expression of inflammation-related genes was confirmed by real-time qPCR. The expression of inducible nitric oxide synthase (iNOS) and nuclear factor-kappa B (NF-κB) were estimated using immunohistochemistry, and xanthine oxidase inhibitory activity was evaluated using an in vitro assay. The results revealed that compounds A and B decreased inflammation, as was observed by a reduction in the elevation of all the tested markers. In addition, the tested compounds markedly decreased paw swelling, mobilization of inflammatory cells, iNOS-, and NF-κB-immunoreactive cells in a mouse model of paw edema. Interestingly, both compounds were potent xanthine oxidase inhibitors as well as Cox inhibitors with higher activity in favor of compound B providing potential dual acting series of anti-hyperuricemic and anti-inflammatory therapeutic agents.
The Novel Perspectives of Adipokines on Brain Health
Authors: Lee Thomas Ho-yin , Cheng Kenneth King-yip , Hoo Ruby Lai-chong , Siu Parco Ming-fai , Yau Suk-yu
Published: November 2019

First seen as a fat-storage tissue, the adipose tissue is considered as a critical player in the endocrine system. Precisely, adipose tissue can produce an array of bioactive factors, including cytokines, lipids, and extracellular vesicles, which target various systemic organ systems to regulate metabolism, homeostasis, and immune response. The global effects of adipokines on metabolic events are well defined, but their impacts on brain function and pathology remain poorly defined. Receptors of adipokines are widely expressed in the brain. Mounting evidence has shown that leptin and adiponectin can cross the blood–brain barrier, while evidence for newly identified adipokines is limited. Significantly, adipocyte secretion is liable to nutritional and metabolic states, where defective circuitry, impaired neuroplasticity, and elevated neuroinflammation are symptomatic. Essentially, neurotrophic and anti-inflammatory properties of adipokines underlie their neuroprotective roles in neurodegenerative diseases. Besides, adipocyte-secreted lipids in the bloodstream can act endocrine on the distant organs. In this article, we have reviewed five adipokines (leptin, adiponectin, chemerin, apelin, visfatin) and two lipokines (palmitoleic acid and lysophosphatidic acid) on their roles involving in eating behavior, neurotrophic and neuroprotective factors in the brain. Understanding and regulating these adipokines can lead to novel therapeutic strategies to counteract metabolic associated eating disorders and neurodegenerative diseases, thus promote brain health.
Maternal Platelets—Friend or Foe of the Human Placenta?
Authors: Moser Gerit , Guettler Jacqueline , Forstner Désirée , Gauster Martin
Published: November 2019

Human pregnancy relies on hemochorial placentation, including implantation of the blastocyst and deep invasion of fetal trophoblast cells into maternal uterine blood vessels, enabling direct contact of maternal blood with placental villi. Hemochorial placentation requires fast and reliable hemostasis to guarantee survival of the mother, but also for the neonates. During human pregnancy, maternal platelet count decreases gradually from first, to second, and third trimester. In addition to hemodilution, accelerated platelet sequestration and consumption in the placental circulation may contribute to a decline of platelet count throughout gestation. Local stasis, turbulences, or damage of the syncytiotrophoblast layer can activate maternal platelets within the placental intervillous space and result in formation of fibrin-type fibrinoid. Perivillous fibrinoid is a regular constituent of the normal placenta which is considered to be an important regulator of intervillous hemodynamics, as well as having a role in shaping the developing villous trees. However, exaggerated activation of platelets at the maternal-fetal interface can provoke inflammasome activation in the placental trophoblast, and enhance formation of circulating platelet-monocyte aggregates, resulting in sterile inflammation of the placenta and a systemic inflammatory response in the mother. Hence, the degree of activation determines whether maternal platelets are a friend or foe of the human placenta. Exaggerated activation of maternal platelets can either directly cause or propagate the disease process in placenta-associated pregnancy pathologies, such as preeclampsia.
Titania Nanofiber Scaffolds with Enhanced Biointegration Activity—Preliminary In Vitro Studies
Authors: Ehlert Michalina , Roszek Katarzyna , Jędrzejewski Tomasz , Bartmański Michał , Radtke Aleksandra
Published: November 2019

The increasing need for novel bone replacement materials has been driving numerous studies on modifying their surface to stimulate osteogenic cells expansion and to accelerate bone tissue regeneration. The goal of the presented study was to optimize the production of titania-based bioactive materials with high porosity and defined nanostructure, which supports the cell viability and growth. We have chosen to our experiments TiO2 nanofibers, produced by chemical oxidation of Ti6Al4V alloy. Fibrous nanocoatings were characterized structurally (X-ray diffraction (XRD)) and morphologically (scanning electron microscopy (SEM)). The wettability of the coatings and their mechanical properties were also evaluated. We have investigated the direct influence of the modified titanium alloy surfaces on the survival and proliferation of mesenchymal stem cells derived from adipose tissue (ADSCs). In parallel, proliferation of bone tissue cells—human osteoblasts MG-63 and connective tissue cells - mouse fibroblasts L929, as well as cell viability in co-cultures (osteoblasts/ADSCs and fibroblasts/ADSCs has been studied. The results of our experiments proved that among all tested nanofibrous coatings, the amorphous titania-based ones were the most optimal scaffolds for the integration and proliferation of ADSCs, fibroblasts, and osteoblasts. Thus, we postulated these scaffolds to have the osteopromotional potential. However, from the co-culture experiments it can be concluded that ADSCs have the ability to functionalize the initially unfavorable surface, and make it suitable for more specialized and demanding cells.
Cell-Free Nucleic Acids
Authors: Nagy Balint
Published: November 2019

The discovery of cell-free DNA (cfDNA) dates back to 1948, when Mandel and Metais found it in the sera of cancer patients [...]
Oligomerization Affects the Ability of Human Cyclase-Associated Proteins 1 and 2 to Promote Actin Severing by Cofilins
Authors: Purde Vedud , Busch Florian , Kudryashova Elena , Wysocki Vicki H. , Kudryashov Dmitri S.
Published: November 2019

Actin-depolymerizing factor (ADF)/cofilins accelerate actin turnover by severing aged actin filaments and promoting the dissociation of actin subunits. In the cell, ADF/cofilins are assisted by other proteins, among which cyclase-associated proteins 1 and 2 (CAP1,2) are particularly important. The N-terminal half of CAP has been shown to promote actin filament dynamics by enhancing ADF-/cofilin-mediated actin severing, while the central and C-terminal domains are involved in recharging the depolymerized ADP–G-actin/cofilin complexes with ATP and profilin. We analyzed the ability of the N-terminal fragments of human CAP1 and CAP2 to assist human isoforms of “muscle” (CFL2) and “non-muscle” (CFL1) cofilins in accelerating actin dynamics. By conducting bulk actin depolymerization assays and monitoring single-filament severing by total internal reflection fluorescence (TIRF) microscopy, we found that the N-terminal domains of both isoforms enhanced cofilin-mediated severing and depolymerization at similar rates. According to our analytical sedimentation and native mass spectrometry data, the N-terminal recombinant fragments of both human CAP isoforms form tetramers. Replacement of the original oligomerization domain of CAPs with artificial coiled-coil sequences of known oligomerization patterns showed that the activity of the proteins is directly proportional to the stoichiometry of their oligomerization; i.e., tetramers and trimers are more potent than dimers, which are more effective than monomers. Along with higher binding affinities of the higher-order oligomers to actin, this observation suggests that the mechanism of actin severing and depolymerization involves simultaneous or consequent and coordinated binding of more than one N-CAP domain to F-actin/cofilin complexes.
Safety Assessment of Tunnel Portals for Site Selection Based on Spatial Information Geoprocessing
Authors: Wang Iau-Teh
Published: November 2019

The evaluation of portal locations for mountain tunnels is among the most crucial considerations during route selection and structural layout planning. The development of spatial information technology has provided a more objective approach for assessing the slope stability of potential portal sites. The simulations in such studies have been performed to evaluate potential hazards and slope stability. However, potential instabilities resulting from excavation are seldom considered in these studies. Therefore, a method based on spatial information technology was developed in this study for considering the potential impact of the direction and depth of excavations on portal stability. An analysis method for an infinite slope was integrated into the geographical information system for evaluating the stability of critical wedges. The proposed method provides a reasonable estimation comparable with that provided by the conventional slice method. The results of applying this method to six mountain tunnel portals where slope instability occurred during construction indicate that the actual outcomes agreed with the predicted outcomes. For potential portal site evaluation, the proposed method facilitates the rapid estimation of safety factors for various slope designations, which is useful for site selection.
Genetic Modifiers at the Crossroads of Personalised Medicine for Haemoglobinopathies
Authors: Stephanou Coralea , Tamana Stella , Minaidou Anna , Papasavva Panayiota , Kleanthous Marina , Kountouris Petros
Published: November 2019

Haemoglobinopathies are common monogenic disorders with diverse clinical manifestations, partly attributed to the influence of modifier genes. Recent years have seen enormous growth in the amount of genetic data, instigating the need for ranking methods to identify candidate genes with strong modifying effects. Here, we present the first evidence-based gene ranking metric (IthaScore) for haemoglobinopathy-specific phenotypes by utilising curated data in the IthaGenes database. IthaScore successfully reflects current knowledge for well-established disease modifiers, while it can be dynamically updated with emerging evidence. Protein–protein interaction (PPI) network analysis and functional enrichment analysis were employed to identify new potential disease modifiers and to evaluate the biological profiles of selected phenotypes. The most relevant gene ontology (GO) and pathway gene annotations for (a) haemoglobin (Hb) F levels/Hb F response to hydroxyurea included urea cycle, arginine metabolism and vascular endothelial growth factor receptor (VEGFR) signalling, (b) response to iron chelators included xenobiotic metabolism and glucuronidation, and (c) stroke included cytokine signalling and inflammatory reactions. Our findings demonstrate the capacity of IthaGenes, together with dynamic gene ranking, to expand knowledge on the genetic and molecular basis of phenotypic variation in haemoglobinopathies and to identify additional candidate genes to potentially inform and improve diagnosis, prognosis and therapeutic management.
Relationship between Lower Urinary Tract Symptoms and Prostatic Urethral Stiffness Using Strain Elastography: Initial Experiences
Authors: Kwon Jong Kyou , Kim Do Kyung , Lee Joo Yong , Kim Jong Won , Cho Kang Su
Published: November 2019

We attempted to visualize the periurethral stiffness of prostatic urethras using strain elastography in the midsagittal plane of transrectal ultrasonography (TRUS) and to evaluate periurethral stiffness patterns in relation to lower urinary tract symptoms (LUTS). A total of 250 men were enrolled. The stiffness patterns of the entire prostate and individual zones were evaluated using strain elastography during a TRUS examination. After excluding 69 men with inappropriate elastography images, subjects were divided according to periurethral stiffness into either group A (low periurethral stiffness, N = 80) or group B (high periurethral stiffness, N = 101). There were significant differences in patient age (p = 0.022), transitional zone volume (p = 0.001), transitional zone index (p = 0.33), total international prostate symptom score (IPSS) (p < 0.001), IPSS-voiding subscore (p < 0.001), IPSS-storage subscore (p < 0.001), and quality of life (QoL) score (p = 0.002) between groups A and B. After adjusting for relevant variables, significant differences in total IPSS, IPSS-voiding subscore, and QoL score were maintained. Men with high periurethral stiffness were associated with worse urinary symptoms than those with low periurethral stiffness, suggesting that periurethral stiffness might play an important role in the development of LUTS.
Fracture Resistance of Partial Indirect Restorations Made with CAD/CAM Technology. A Systematic Review and Meta-Analysis
Authors: Amesti-Garaizabal Amaia , Agustín-Panadero Rubén , Verdejo-Solá Blanca , Fons-Font Antonio , Fernández-Estevan Lucía , Montiel-Company Jose , Solá-Ruíz María Fernanda
Published: November 2019

Background: The aim of this systematic review and meta-analysis was to determine the fracture resistance and survival rate of partial indirect restorations inlays, onlays, and overlays fabricated using computer-aided design and computer-aided manufacturing (CAD-CAM) technology from ceramics, composite resin, resin nanoceramic, or hybrid ceramic and to analyze the influence of proximal box elevation on fracture resistance. Materials and methods: This systematic review was based on guidelines proposed by the preferred reporting items for systematic reviews and meta-analyses (PRISMA). An electronic search was conducted in databases US National Library of Medicine National Institutes of Health (PubMed), Scopus, Web of Science (WOS), and Embase. In vitro trials published during the last 10 years were included in the review. Results: Applying inclusion criteria based on the review’s population, intervention, comparison, outcome (PICO) question, 13 articles were selected. Meta-analysis by restoration type estimated the fracture resistance of inlays to be 1923.45 Newtons (N); of onlays 1644 N and of overlays 1383.6 N. Meta-analysis by restoration material obtained an estimated fracture resistance for ceramic of 1529.5 N, for composite resin of 1600 Ne, for resin nanoceramic 2478.7 N, and hybrid ceramic 2108 N. Conclusions: Resin nanoceramic inlays present significantly higher fracture resistance values. Proximal box elevation does not exert any influence on the fracture resistance of indirect restorations.
Internal Fixation of Osteochondritis Dissecans of the Knee Leads to Good Long-Term Outcomes and High Degree of Healing without Differences between Fixation Devices
Authors: Perelli Simone , Molina Romoli Agustín Rubén , Costa-Paz Matías , Erquicia Juan Ignacio , Gelber Pablo Eduardo , Monllau Juan Carlos
Published: November 2019

The aim of the present study is to describe results at long-term follow-up of internal fixation of unstable Osteochondritis Dissecans (OCD) achieved with three different fixation devices in skeletally mature knees. A retrospective cohort study was performed at 5 to 19 years follow-up. Patient-reported questionnaires were collected at the final follow-up. Postoperative X-rays and MRIs were evaluated for healing of the lesion and articular degeneration. An arthroscopic second look was performed in 74.3% of the cases. Failures were reported as reintervention to address the osteochondral lesion or poor functional outcomes at the last follow-up. A total of 39 subjects with a median follow-up of 10.7 years were included. Herbert screws were used in 51.2% of the cases, bioabsorbable nails in 25.7% of the cases and cannulated screws in 23.1% of the cases. No differences were observed in terms of the clinical score (International Knee Documentation Committee (IKDC) p = 0.211; Lysholm p = 0.197), radiographic union (p = 0.102) or radiographic degeneration (p = 0.238) between the three different fixation devices. Arthroscopic second look found complete stability of the lesions in all 29 cases evaluated. The mean postoperative Lysholm score was 83 (range = 33–100) and IKDC score was 79 (range = 39–100). Radiographic union was seen in 74% of the cases. Lack of radiographic union was correlated with worst functional scores. A failure rate of 20.5% was found: four reinterventions were performed, and four patients had poor scores at last follow up. This study shows that internal fixation of condylar OCD in skeletally mature patients provides good long-term clinical results and a high degree of healing regardless of the dimensions of the lesion and type of fixation.
Circulating Endometrial Cells: A New Source of Information on Endometriosis Dynamics
Authors: Pospisilova Eliska , Kiss Imrich , Souckova Helena , Tomes Pavel , Spicka Jan , Matkowski Rafal , Jedryka Marcin , Ferrero Simone , Bobek Vladimir , Kolostova Katarina
Published: November 2019

The focus of the presented work was to isolate and characterize circulating endometrial cells (CECs) enriched from peripheral blood (PB) of patients with diagnosed endometriosis. The molecular characteristics of CECs could be supportive for an understanding of endometriosis pathogenesis and treatment decisions in the future. Material and Methods: Blood samples (n = 423) were tested for CECs presence. Subsequently, gene expression analysis (GEA) was carried out for CECs. In parallel, CECs presence and characteristics were tested during menstrual cycle (MC) phases in 11 patients. CECs were enriched by size-based separation. Results: CECs were present in 78.4% of the tested blood samples. In line with the revised American Fertility Society (rAFS) classification, CECs presence was confirmed in all the acknowledged endometriosis stages: minimal, mild, moderate, and severe. Surprisingly, CECs negativity rate was also reported for severe disease in 21.1% of cases. The CECs captured during MC phases displayed different cytomorphology, including epithelial, stromal, and stem cell-like characteristics. The highest CECs numbers were detected in the mid-secretory phase of MC, which corresponds to uterine lining decidualization. CECs captured during mid-secretory periods expressed genes KRT18, NANOG, and VIM in higher amounts when compared to the proliferative phase of MC, where genes KRT19 and ESR1 were mostly elevated. GEA of the super-positive CECs samples (1000 CECs/8 mL PB) revealed high expression of genes KRT18, VIM, NANOG, and FLT1. The expression of these genes was also elevated in the endometriosis tissue samples and endometrioma. Conclusion: The panel of the identified CEC genes could be tested in a prospective manner to confirm the role of CECs in endometriosis pathogenesis and diagnostics.
The Effects of Postoperative Astaxanthin Administration on Nasal Mucosa Wound Healing
Authors: Manciula Lavinia-Gianina , Berce Cristian , Tabaran Flaviu , Trombitaș Veronica , Albu Silviu
Published: November 2019

Background: Wound healing of the nasal mucosa after endoscopic sinus surgery (ESS) is frequently complicated by scaring and consequently recurrences are encountered. Methods of optimizing results have been sought. In the present study we evaluated the effects of a powerful antioxidant, astaxanthin, on nasal mucosa healing after surgery, comparing it to the extensively studied properties of dexamethasone. Materials and Methods: 63 Wistar rats were used. The nasal mucosa from one side was damaged employing the brushing method. They were randomly divided into three experimental groups, one treated with astaxanthin, the second treated with dexamethasone and the third one acted as the control and was given normal saline. The rats were killed on days 5, 14 and 28 following injury. We observed the temporal evolution of the wound healing process and quantified the results by assessing four parameters: the epithelial thickness index (ETI), the subepithelial thickness index (STI), the goblet cell count and the subepithelial fibrosis index (SFI). Results: At 28 days, the ETI was significantly lower in the astaxanthin group (p < 0.05) compared to the other two groups. The STI was also lower in the astaxanthin group (p < 0.05), but comparable to the dexamethasone group at 28 days. The goblet cell count was higher in the astaxanthin group. The SFI had similar results in both dexamethasone and astaxanthin groups, with lower values compared to the control group. In the astaxanthin group there was no synechia formation. Conclusion: Astaxanthin given in the post injury period significantly decreases fibrosis, inhibits synechia development and significantly decreases subepithelial fibrosis. Moreover, it has no general or local toxic effects.
Conversion Surgery for Advanced Pancreatic Cancer
Authors: Hank Thomas , Strobel Oliver
Published: November 2019

While primarily unresectable locally advanced pancreatic cancer (LAPC) used to be an indication for palliative therapy, a strategy of neoadjuvant therapy (NAT) and conversion surgery is being increasingly used after more effective chemotherapy regimens have become available for pancreatic ductal adenocarcinoma. While high-level evidence from prospective studies is still sparse, several large retrospective studies have recently reported their experience with NAT and conversion surgery for LAPC. This review aims to provide a current overview about different NAT regimens, conversion rates, survival outcomes and determinants of post-resection outcomes, as well as surgical strategies in the context of conversion surgery after NAT. FOLFIRINOX is the predominant regimen used and associated with the highest reported conversion rates. Conversion rates considerably vary between less than 5% and more than half of the study population with heterogeneous long-term outcomes, owing to a lack of intention-to-treat analyses in most studies and a high heterogeneity in resectability criteria, treatment strategies, and reporting among studies. Since radiological criteria of local resectability are no longer applicable after NAT, patients without progressive disease should undergo surgical exploration. Surgery after NAT has to be aimed at local radicality around the peripancreatic vessels and should be performed in expert centers. Future studies in this rapidly evolving field need to be prospective, analyze intention-to-treat populations, report stringent and objective inclusion criteria and criteria for resection. Innovative regimens for NAT in combination with a radical surgical approach hold high promise for patients with LAPC in the future.
The Influence of Thrust Force on the Vitality of Bone Chips Harvested for Autologous Augmentation during Dental Implantation
Authors: Ben Achour Anas , Petto Carola , Meißner Heike , Hipp Dominik , Nestler Andreas , Lauer Günter , Teicher Uwe
Published: November 2019

Bone drill chips that are collected during implant site preparation can be reused as autologous bone-grafting material for alveolar ridge augmentation. This study characterized five market-leading implant drill sets regarding their geometric properties and ability to produce vital bone chips. The drill geometry of each tool of five commercial implant drill sets was characterized while using optical profile projector devices and SEM. Bone chips were collected during the in vitro preparation of porcine jaw bone with the various drill sets. Produced bone chip masses were measured. The bone chips were cultured in vitro and the number of outgrown cells was determined and measurand for vitality. Furthermore, the thrust force and cutting torque were recorded to examine the mechanical loads of the manual drilling process. The tool geometry and set configuration of one out of five implant drill sets appears to be superior regarding chip mass, vitality, and thrust force. It could be proven that there is a correlation between vitality and thrust force. The thrust force is influenced by the cutting behavior of the tool, which in turn depends on the geometry of the tool. The tool geometry has an influence on the vitality of the augmentation material due to this relationship.
Resistance Switching Behavior in Rectangle-Nano-Pattern SrTiO3 Induced by Simple Annealing
Authors: Liao Xiaxia , Zhang Yufeng , Wang Jiaou , Kang Junyong , Zhang Jinbin , Wang Jizheng , Zheng Jincheng , Wang Huiqiong
Published: November 2019

The tunability of semi-conductivity in SrTiO3 single crystal substrates has been realized by a simple encapsulated annealing method under argon atmosphere. This high temperature annealing-induced property changes are characterized by the transmission spectra, scanning electron microscopy (SEM) and synchrotron-based X-ray absorption (XAS). We find the optical property is strongly influenced by the annealing time (with significant decrease of transmittance). A sub gap absorption at ~427 nm is detected which is attributed to the introduction of oxygen vacancy. Interestingly, in the SEM images, annealing-induced regularly rectangle nano-patterns are directly observed which is contributed to the conducting filaments. The XAS of O K-edge spectra shows the changes of electronic structure by annealing. Very importantly, resistance switching response is displayed in the annealed SrTiO3 single crystal. This suggests a possible simplified route to tune the conductivity of SrTiO3 and further develop novel resistance switching materials.
Properties of Arsenic–Doped ZnTe Thin Films as a Back Contact for CdTe Solar Cells
Authors: Oklobia Ochai , Kartopu Giray , J. C. Irvine Stuart
Published: November 2019

As-doped polycrystalline ZnTe layers grown by metalorganic chemical vapor deposition (MOCVD) have been investigated as a back contact for CdTe solar cells. While undoped ZnTe films were essentially insulating, the doped layers showed significant rise in conductivity with increasing As concentration. High p-type carrier densities up 4.5 × 1018 cm−3 was measured by the Hall-effect in heavily doped ZnTe:As films, displaying electrical properties comparable to epitaxial ZnTe single crystalline thin films in the literature. Device incorporation with as-deposited ZnTe:As yielded lower photovoltaic (PV) performance compared to reference devices, due to losses in the open-circuit potential (VOC) and fill factor (FF) related to reducing p-type doping density (NA) in the absorber layer. Some minor recovery observed in absorber doping following a Cl-free post–ZnTe:As deposition anneal in hydrogen at 420 °C contributed to a slight improvement in VOC and NA, highlighting the significance of back contact activation. A mild CdCl2 activation process on the ZnTe:As back contact layer via a sacrificial CdS cap layer has been assessed to suppress Zn losses, which occur in the case of standard CdCl2 anneal treatments (CHT) via formation of volatile ZnCl2. The CdS sacrificial cap was effective in minimising the Zn loss. Compared to untreated and non-capped, mild CHT processed ZnTe:As back contacted devices, mild CHT with a CdS barrier showed the highest recovery in absorber doping and an ~10 mV gain in VOC, with the best cell efficiency approaching the baseline devices.
Growth of β-NaYF4:Eu3+ Crystals by the Solvothermal Method with the Aid of Oleic Acid and Their Photoluminescence Properties
Authors: Huang Jianhua , Wang Xiaojing , Shao An , Du Guoping , Chen Nan
Published: November 2019

Lanthanide-doped hexagonal β-NaYF4 crystals have received much attention in recent years due to their excellent photoluminescence properties. However, lanthanide-doped β-NaYF4 crystals with micron and submicron scales as well as uniform morphology have received less attention. In this study, Eu3+-doped β-NaYF4 (β-NaYF4:Eu3+) crystals of micron and submicron size scales were synthesized using the solvothermal method with ethylene glycol as the solvent. The β-NaYF4:Eu3+ crystals were highly crystallized. A comparison of the characteristics of the β-NaYF4:Eu3+ crystals synthesized with and without the use of oleic acid as a surfactant was conducted. It was found that the utilization of oleic acid as a surfactant during their synthesis greatly decreased their particle size from micron to submicron scale, while adding a small amount of ethanol further reduced their particle size. In addition, they exhibited much smoother surfaces and more uniform morphologies, which were hexagonal prism bipyramids. The microstructural characteristics and photoluminescence properties of the β-NaYF4:Eu3+ crystals were studied in detail. Results showed that β-NaYF4:Eu3+ crystals prepared with the aid of oleic acid as a surfactant during their synthesis exhibited stronger photoluminescence.
Effects of Water Exposure on the Interfacial Bond between an Epoxy Resin Coating and a Concrete Substrate
Authors: Won Boreum , Kim Min Ook , Park Sangmin , Yi Jin-Hak
Published: November 2019

Interfacial bond properties of six different epoxy resins used to coat submerged concrete structures were investigated. Test variables included coating type, coating equipment, and underwater curing time. Coating thickness and pull-off bond strength were measured using commercially available test equipment. Coating thickness and bond strength varied greatly depending on the manufacturer. The standard (control) coating equipment positively influenced the bond strength compared to other equipment. The effect of curing time on the bond properties was not significant within the range of 24 to 72 h. Lastly, some important considerations for the underwater coating of actual marine and coastal concrete structures were discussed, and suggestions for future research are presented.
Effect of Heat Treatment on the Phase Composition and Corrosion Resistance of 321 SS Welded Joints Produced by a Defocused Laser Beam
Authors: Kuryntsev Sergey Vyacheslavovich
Published: November 2019

The effect of heat treatment of welded joints made of steel 321 on corrosion resistance, phase composition, residual stresses, and distribution of alloying elements was studied using optical microscope (OM) and scanning electron microscope (SEM), electron dispersive spectroscopy (EDS), X-ray diffraction (XRD), and intergranular corrosion testing (IGC). Samples previously obtained by the authors using defocused laser beam, which led to the formation of directionally crystallized austenite with lathy and skeletal δ-ferrite, were investigated. Based on X-ray diffraction studies in the base metal, the maximum number of peaks of various phases was presented, which decreased after exposure to the heating effect of the welding process and subsequent heat treatment. The distribution of alloying elements, in particular, Ti and Si, was significantly affected by heat treatment depending on the regimes. A spot chemical analysis showed that the nickel content differs in δ-ferrite and austenite by 1.5%–2% whereas the chromium content in these phases is not significantly different. Tests have shown that all samples have high resistance to intergranular corrosion, which can be explained by the insufficient dissolution of titanium carbides in austenite and the absence of chromium carbides formation along austenite grain boundaries, due to high cooling rates when welding by a defocused laser beam, and as a result, the high δ-ferrite content in which chromium dissolves.
On the Unitary Representations of the Braid Group B6
Authors: Dally Malak M. , Abdulrahim Mohammad N.
Published: November 2019

We consider a non-abelian leakage-free qudit system that consists of two qubits each composed of three anyons. For this system, we need to have a non-abelian four dimensional unitary representation of the braid group B 6 to obtain a totally leakage-free braiding. The obtained representation is denoted by ρ . We first prove that ρ is irreducible. Next, we find the points y ∈ C * at which the representation ρ is equivalent to the tensor product of a one dimensional representation χ ( y ) and μ ^ 6 ( ± i ) , an irreducible four dimensional representation of the braid group B 6 . The representation μ ^ 6 ( ± i ) was constructed by E. Formanek to classify the irreducible representations of the braid group B n of low degree. Finally, we prove that the representation χ ( y ) ⊗ μ ^ 6 ( ± i ) is a unitary relative to a hermitian positive definite matrix.
Numerical Analysis of Thermal Non-Equilibrium in Porous Medium Subjected to Internal Heating
Authors: Badruddin Irfan Anjum
Published: November 2019

Thermal non-equilibrium in porous medium is a prevailing condition when discrepancy of temperature exists between the two phases. The solution of a thermal non-equilibrium model requires that the two heat transport equations corresponding to fluid and solid phases, can be solved separately, which, in turn, provides the information of temperature variations of fluid as well as solid phases of the porous domain. A new method is proposed in the current article to solve the energy equations of the thermal non-equilibrium condition in a porous square cavity. The proposed method is able to predict the thermal equilibrium as well as thermal non-equilibrium accurately. The proposed method is used to investigate the heat transfer through the porous cavity subjected to two different boundary conditions with a heating strip being placed inside the porous cavity. It was found that the new method predicted the heat and fluid flow behavior accurately for the previously mentioned case studies. It is noted that the heat transfer is higher when the heating strip is placed toward the cold surface. The Nusselt number at the bottom of the strip toward the right side is almost 10 times higher than that of the left side of the strip.
Energy-Based Control and LMI-Based Control for a Quadrotor Transporting a Payload
Authors: Guerrero-Sánchez María-Eusebia , Hernández-González Omar , Lozano Rogelio , García-Beltrán Carlos-D. , Valencia-Palomo Guillermo , López-Estrada Francisco-R.
Published: November 2019

This paper presents the control of a quadrotor with a cable-suspended payload. The proposed control structure is a hierarchical scheme consisting of an energy-based control (EBC) to stabilize the vehicle translational dynamics and to attenuate the payload oscillation, together with a nonlinear state feedback controller based on an linear matrix inequality (LMI) to control the quadrotor rotational dynamics. The payload swing control is based on an energy approach and the passivity properties of the system’s translational dynamics. The main advantage of the proposed EBC strategy is that it does not require excessive computations and complex partial differential equations (PDEs) for implementing the control algorithm. We present a new methodology for using an LMI to synthesize the controller gains for Lipschitz nonlinear systems with larger Lipschitz constants than other classical techniques based on LMIs. This theoretical approach is applied to the quadrotor rotational dynamics. Stability proofs based on the Lyapunov theory for the controller design are presented. The designed control scheme allows for the stabilization of the system in all its states for the three-dimensional case. Numerical simulations demonstrating the effectiveness of the controller are provided.
Fuzzy Logic and Its Uses in Finance: A Systematic Review Exploring Its Potential to Deal with Banking Crises
Authors: Sanchez-Roger Marc , Oliver-Alfonso María Dolores , Sanchís-Pedregosa Carlos
Published: November 2019

The major success of fuzzy logic in the field of remote control opened the door to its application in many other fields, including finance. However, there has not been an updated and comprehensive literature review on the uses of fuzzy logic in the financial field. For that reason, this study attempts to critically examine fuzzy logic as an effective, useful method to be applied to financial research and, particularly, to the management of banking crises. The data sources were Web of Science and Scopus, followed by an assessment of the records according to pre-established criteria and an arrangement of the information in two main axes: financial markets and corporate finance. A major finding of this analysis is that fuzzy logic has not yet been used to address banking crises or as an alternative to ensure the resolvability of banks while minimizing the impact on the real economy. Therefore, we consider this article relevant for supervisory and regulatory bodies, as well as for banks and academic researchers, since it opens the door to several new research axes on banking crisis analyses using artificial intelligence techniques.
A Compact Representation of Preferences in Multiple Criteria Optimization Problems
Authors: Salas-Molina Francisco , Pla-Santamaria David , Garcia-Bernabeu Ana , Reig-Mullor Javier
Published: November 2019

A critical step in multiple criteria optimization is setting the preferences for all the criteria under consideration. Several methodologies have been proposed to compute the relative priority of criteria when preference relations can be expressed either by ordinal or by cardinal information. The analytic hierarchy process introduces relative priority levels and cardinal preferences. Lexicographical orders combine both ordinal and cardinal preferences and present the additional difficulty of establishing strict priority levels. To enhance the process of setting preferences, we propose a compact representation that subsumes the most common preference schemes in a single algebraic object. We use this representation to discuss the main properties of preferences within the context of multiple criteria optimization.
Optimal Uncertain Controls for Cash Holding Problems
Authors: Wang Zhengyan , Xu Guanghua , Zhao Peibiao
Published: November 2019

Determining whether an enterprise has target holdings and figuring out how to reasonably determine these cash holdings are common problems faced by all enterprises. This paper first establishes an uncertain optimal cash holdings model with a security area constraint, and then proves that the model is a typical bang–bang control model. The control variables in the model can be expressed as a symbolic function. Then, under the specific objective function, the optimal cash holdings are discussedfor two casesincluding whether to consider transaction costs or not. Finally, the applicability of the model is verified by specific examples, and the influences of factors, including risky asset returns and the transaction cost of unit securities on decision-making results are discussed.