bluuebunny/crossref_metadata_2025 huggingface dataset:
Detection of the oil-producing microalga Botryococcus braunii in natural freshwater environments by targeting the hydrocarbon biosynthesis gene SSL-3
Authors: Hirano Kotaro , Hara Takuya , Ardianor  , Nugroho Rudy Agung , Segah Hendrik , Takayama Naru , Sulmin Gumiri , Komai Yukio , Okada Shigeru , Kawamura Koji
Published: November 2019

The green microalga Botryococcus braunii produces hydrocarbon oils at 25–75% of its dry weight and is a promising source of biofuel feedstock. Few studies have examined this species’ ecology in natural habitats, and few wild genetic resources have been collected due to difficulties caused by its low abundance in nature. This study aimed to develop a real-time PCR assay for specific detection and quantification of this alga in natural environments and to quantify spatiotemporal variations of wild B. braunii populations in a tropical pond. We designed PCR primers toward the hydrocarbon biosynthesis gene SSL-3 and examined amplification specificity and PCR efficiency with 70 wild strains newly isolated from various environments. The results demonstrated that this PCR assay specifically amplified B. braunii DNA, especially that of B-race strains, and can be widely used to detect wild B. braunii strains in temperate and tropical habitats. Field-testing in a tropical pond suggested a diurnal change in the abundance of B. braunii in surface water and found B. braunii not only in surface water, but also at 1–1.5 m deep and in bottom sediments. This method can contribute to efficient genetic resource exploitations and may also help elucidate the unknown ecology of B. braunii.
Prefrontal and posterior parietal contributions to the perceptual awareness of touch
Authors: Rullmann M. , Preusser S. , Pleger B.
Published: November 2019

Which brain regions contribute to the perceptual awareness of touch remains largely unclear. We collected structural magnetic resonance imaging scans and neurological examination reports of 70 patients with brain injuries or stroke in S1 extending into adjacent parietal, temporal or pre-/frontal regions. We applied voxel-based lesion-symptom mapping to identify brain areas that overlap with an impaired touch perception (i.e., hypoesthesia). As expected, patients with hypoesthesia (n = 43) presented lesions in all Brodmann areas in S1 on postcentral gyrus (BA 1, 2, 3a, 3b). At the anterior border to BA 3b, we additionally identified motor area BA 4p in association with hypoesthesia, as well as further ventrally the ventral premotor cortex (BA 6, BA 44), assumed to be involved in whole-body perception. At the posterior border to S1, we found hypoesthesia associated effects in attention-related areas such as the inferior parietal lobe and intraparietal sulcus. Downstream to S1, we replicated previously reported lesion-hypoesthesia associations in the parietal operculum and insular cortex (i.e., ventral pathway of somatosensory processing). The present findings extend this pathway from S1 to the insular cortex by prefrontal and posterior parietal areas involved in multisensory integration and attention processes.
Electrodeposition behavior of homoleptic transition metal acetonitrile complexes interrogated with piezoelectric gravimetry
Authors: Sconyers David J. , Blakemore James D.
Published: November 2019

Homoleptic first-row transition metal acetonitrile complexes are shown to be precursors for metal electrodeposition from homogeneous solutions.
Rapid differentiation of Campylobacter jejuni cell wall mutants using Raman spectroscopy, SERS and mass spectrometry combined with chemometrics
Authors: Chisanga Malama , Linton Dennis , Muhamadali Howbeer , Ellis David I. , Kimber Richard L. , Mironov Aleksandr , Goodacre Royston
Published: November 2019

SERS was developed for intercellular and intracellular analyses. Using a series of cell wall mutants in C. jejuni we show cell wall versus cytoplasm differences.
Lactate monitoring in droplet microfluidics: a cautionary tale in assay miniaturisation
Authors: Leong Chi Leng , Coleman Sharon , Nightingale Adrian M. , Hassan Sammer-ul , Voegeli David , Boutelle Martyn G. , Niu Xize
Published: November 2019

We present the development of a droplet-based device for real-time continuous lactate measurement, highlighting how protocols from established homogeneous enzymatic assays require careful re-optimisation before transferring to microfluidic systems.
Tailored ultra-small Prussian blue-based nanoparticles for MRI imaging and combined photothermal/photoacoustic theranostics
Authors: Fétiveau Lucile , Paul Gabriella , Nicolas-Boluda Alba , Volatron Jeanne , George Riya , Laurent Sophie , Muller Robert , Sancey Lucie , Mejanelle Philippe , Gloter Alexandre , Gazeau Florence , Catala Laure
Published: November 2019

Ultrasmall sub-10 nm nanoparticles of Prussian blue analogues incorporating GdIII ions at their periphery revealed longitudinal relaxivities above 40 mM−1 s−1 per GdIII regardless of the nature of the core and the polymer coating.
Polymerization-driven successive collapse of DNA dominoes enabling highly sensitive cancer gene diagnosis
Authors: Dong Haiyan , Lu Bo , Wang Jie , Xie Jingjing , Liu Kuancan , Jia Lee , Zhuang Junyang
Published: November 2019

A novel fluorescence assay method based on a polymerization-driven DNA dominoes collapse (PDDC) strategy was designed for sensitive cancer gene diagnosis.
A zinc phthalocyanine–benzoperylenetriimide conjugate for solvent dependent ultrafast energy vs. electron transfer
Authors: Navarro-Pérez Valeria , Gutiérrez-Vílchez Ana M. , Ortiz Javier , Sastre-Santos Ángela , Fernández-Lázaro Fernando , Seetharaman Sairaman , Duffy M. J. , Karr Paul A. , D’Souza Francis
Published: November 2019

Ultrafast energy and electron transfer as a function of solvent polarity has been demonstrated using a femtosecond transient absorption technique in a zinc phthalocyanine–benzoperylenetriimide conjugate.
NIRF turn-on nanoparticles based on the tumor microenvironment for monitoring intracellular protein delivery
Authors: Xu Huaibao , Wang Yi , Pei Zhichao , Ji Wei , Pei Yuxin
Published: November 2019

A dual responsive NIRF turn-on protein delivery system incorporating an NIRF turn-on probe and protein into one single nanoparticle has been constructed.
Hydrophobicity and CH/π-interaction-driven self-assembly of amphiphilic aromatic hydrocarbons into nanosheets
Authors: Nishikawa Tsuyoshi , Narita Hiroki , Ogi Soichiro , Sato Yoshikatsu , Yamaguchi Shigehiro
Published: November 2019

The hydrophobicity and CH/π-interaction-driven self-assembly of an amphiphile that contains a biphenylanthracene group furnishes micrometer-scale nanosheets with a windmill-shaped molecular packing structure in dilute aqueous solution.
Ultrastable tetraphenyl-p-phenylenediamine-based covalent organic frameworks as platforms for high-performance electrochemical supercapacitors
Authors: EL-Mahdy Ahmed F. M. , Mohamed Mohamed Gamal , Mansoure Tharwat Hassan , Yu Hsiao-Hua , Chen Tao , Kuo Shiao-Wei
Published: November 2019

In this study we synthesized two tetraphenyl-p-phenylenediamine-based covalent organic frameworks (TPPDA-TPPyr and TPPDA-TPTPE COFs) for potential use in high-performance electrochemical supercapacitors.
A two-dimensional multi-shelled metal–organic framework and its derived bimetallic N-doped porous carbon for electrocatalytic oxygen reduction
Authors: Xu Yang , Huang Zhehao , Wang Bin , Liang Zuozhong , Zhang Chaochao , Wang Yanzhi , Zhang Wei , Zheng Haoquan , Cao Rui
Published: November 2019

We report a multi-shelled two-dimensional metal–organic framework (MOF), which is transferred to a Co/Ni-embedded bimetallic N-doped porous carbon.
Difluorinative ring expansions of benzo-fused carbocycles and heterocycles are achieved withp-(difluoroiodo)toluene
Authors: Zhao Zhensheng , To Avery J. , Murphy Graham K.
Published: November 2019

Fluorination of exocyclic alkenes and allenes withp-TolIF2gives ring-expanded β,β-difluoridesviaa 1,2-phenyl shift.
Photoredox-catalyzed synthesis of sulfones through deaminative insertion of sulfur dioxide
Authors: Wang Xuefeng , Kuang Yunyan , Ye Shengqing , Wu Jie
Published: November 2019

Katritzky salts are used as the alkyl radical precursors with the insertion of sulfur dioxide under photoredox catalysis, leading to diverse dialkyl sulfones in good to excellent yields.
Highly sensitive mechano-controlled luminescence in polymer films modified by dynamic CuI-based cross-linkers
Authors: Karimata Ayumu , Patil Pradnya H. , Khaskin Eugene , Lapointe Sébastien , Fayzullin Robert R. , Stampoulis Pavlos , Khusnutdinova Julia R.
Published: November 2019

Highly sensitive mechanical stress detection is achieved by using dynamic Cu(i)-based mechanophores as cross-linkers.
Use of pyridazinediones as extracellular cleavable linkers through reversible cysteine conjugation
Authors: Bahou Calise , Spears Richard J. , Aliev Abil E. , Maruani Antoine , Fernandez Marcos , Javaid Faiza , Szijj Peter A. , Baker James R. , Chudasama Vijay
Published: November 2019

Herein we report the potential use of pyridazinediones as novel extracellular cleavable linkers in the context of protein bioconjugates.
Mixed 4f population of Tm adatoms on insulating Cu2N islands
Authors: Coffey David , de la Fuente César , Ciria Miguel , Serrate David , Loth Sebastian , Arnaudas José Ignacio
Published: November 2019

STS of Tm adatoms deposited on insulating Cu2N nanoislands and DFT calculations allow distinguishing the 4f occupancy of the adatoms.
Aqueous systems of a surface active ionic liquid having an aromatic anion: phase behavior, exfoliation of graphene flakes and its hydrogelation
Authors: Kaur Manvir , Singh Gagandeep , Damarla Krishnaiah , Singh Gurbir , Wang Huiyong , Wang Jianji , Aswal Vinod Kumar , Kumar Arvind , Kang Tejwant Singh
Published: November 2019

Surface Active Ionic Liquid (SAIL) induced aqueous phase exfoliation of graphene-flakes and formation of SAIL-graphene-flakes hydrogel.
Critical nucleus of Greek-key-like core of α-synuclein protofibril and its disruption by dopamine and norepinephrine
Authors: Zou Yu , Qian Zhenyu , Gong Yehong , Tang Yiming , Wei Guanghong , Zhang Qingwen
Published: November 2019

Protofibrillar trimer is the critical nucleus for the αS fibril formation, and the tetramer is the minimal stable nucleus. The interactions of DA/NE with αS trimer/tetramer disrupt the backbone H-bonds and destabilize the αS protofibrils.
Ab initio insights into the structural, energetic, electronic, and stability properties of mixed CenZr15−nO30 nanoclusters
Authors: Felício-Sousa Priscilla , Mucelini Johnatan , Zibordi-Besse Larissa , Andriani Karla F. , Seminovski Yohanna , Prati Ronaldo C. , Da Silva Juarez L. F.
Published: November 2019

In this work, we report a DFT investigation combined with Spearman correlation analysis of the energetic, structural and electronic properties of mixed CenZr15−nO30 nanoclusters as a function of the composition (n = 0, 1,…,14, 15).
A general topological network criterion for exploring the structure of icy nanoribbons and monolayers
Authors: Goswami Amrita , Singh Jayant K.
Published: November 2019

We develop intuitive metrics for quantifying complex nucleating systems under confinement.
Surface curvature-dependent adsorption and aggregation of fluorescein isothiocyanate on gold nanoparticles
Authors: Xue Shan , Liu Xinxin , Chen Shun-Li , Gan Wei , Yuan Qunhui
Published: November 2019

Time-dependent TPF measurements revealed the kinetics of the adsorption and aggregation processes of FITC on gold nanoparticles.
Operando soft X-ray emission spectroscopy of the Fe2O3 anode to observe the conversion reaction
Authors: Asakura Daisuke , Nanba Yusuke , Okubo Masashi , Niwa Hideharu , Kiuchi Hisao , Miyawaki Jun , Oshima Masaharu , Hosono Eiji , Harada Yoshihisa
Published: November 2019

Drastic electronic-structure changes in an Fe2O3 thin film anode for a Li-ion battery during discharge (lithiation) and charge (delithiation) processes were observed using operando Fe 2p soft X-ray emission spectroscopy (XES).
On the transition from partial wetting to complete wetting of methanol on graphite
Authors: Prasetyo Luisa , Horikawa Toshihide , Takashima Naoki , Do D. D. , Nicholson D.
Published: November 2019

Excellent agreement with experiment for methanol adsorption on graphitized carbon black at low temperatures by Monte Carlo simulation. Incomplete wetting and complete wetting are observed at a range of temperatures above the triple point.
Analysis of thyme essential oils using gas-phase broadband rotational spectroscopy
Authors: Quesada-Moreno María Mar , Krin Anna , Schnell Melanie
Published: November 2019

A semi-quantitative analysis as well as determination of the structures and internal dynamics of components of two natural essential oils have been carried out using rotational spectroscopy.
In situ synthesis of CuO nanoparticles over functionalized mesoporous silica and their application in catalytic syntheses of symmetrical diselenides
Authors: Das Trisha , Chatterjee Rana , Majee Adinath , Uyama Hiroshi , Morgan David , Nandi Mahasweta
Published: November 2019

A versatile and novel mesoporous silica supported CuO nanoparticle catalyst (nCuO-FMS) and its application in the syntheses of symmetrical diselenides.
Synthesis of a germanosilicate zeolite HPM-12 using a short imidazolium-based dication: structure-direction by charge-to-charge distance matching
Authors: Lu Peng , Gómez-Hortigüela Luis , Gao Zihao , Camblor Miguel A.
Published: November 2019

A short dication selectively directing the crystallization of zeolite HPM-12 (*UOE) adopts an unstable conformation in the zeolite.
A fluorescent “ON–OFF–ON” switch for the selective and sequential detection of Hg2+and I−with applications in imaging using human AGS gastric cancer cells
Authors: Gharami Saswati , Aich Krishnendu , Ghosh Paramita , Patra Lakshman , Murmu Nabendu , Mondal Tapan K.
Published: November 2019

A new fluorescent “on–off–on” probe (BIPQ) probe was designed which displayed selective and sequential detection of Hg2+and I−without interference of other cations or anions.
Mechanical degradation of polyacrylamide at ultra high deformation rates during hydraulic fracturing
Authors: Xiong Boya , Purswani Prakash , Pawlik Taylor , Samineni Laxmicharan , Karpyn Zuleima T. , Zydney Andrew L. , Kumar Manish
Published: November 2019

Degradation of drag reducer polyacrylamide under high volume hydraulic fracturing (HVHF) conditions alters its polymer size, distribution and chemical composition, potentially affecting the toxicity and treatability of the resulting wastewater.
Electrochemical degradation of perfluoroalkyl acids by titanium suboxide anodes
Authors: Wang Yaye , Pierce Randall “David” , Shi Huanhuan , Li Chenguang , Huang Qingguo
Published: November 2019

Effective degradation of eight perfluoroalkyl acids by electrooxidation on titanium suboxide anodes is correlated to their respective molecular structures, offering insight into their degradation behaviors.
Triphenylamine based conjugated microporous polymers for selective photoreduction of CO2 to CO under visible light
Authors: Dai Chunhui , Zhong Lixiang , Gong Xuezhong , Zeng Lei , Xue Can , Li Shuzhou , Liu Bin
Published: November 2019

Two novel triphenylamine based conjugated microporous polymers are designed and synthesized for photocatalytic CO2 reduction using water vapor as electron donor under ambient condition (>420 nm).
Sirtuin inhibition and anti-cancer activities of ethyl 2-benzimidazole-5-carboxylate derivatives
Authors: Yeong K. Y. , Nor Azizi M. I. H. , Berdigaliyev N. , Chen W. N. , Lee W. L. , Shirazi A. N. , Parang K.
Published: November 2019

Sirtuin inhibitor potently inhibits the proliferation of H103 cancer cells.
Introducing nitrogen atoms to amidoalkylindoles: potent and selective cannabinoid type 2 receptor agonists with improved aqueous solubility
Authors: Ji Yue-Yang , Wang Zhi-Long , Pei Fang-Ning , Shi Jun-Jie , Li Jiao-Jiao , Gunosewoyo Hendra , Yang Fan , Tang Jie , Xie Xin , Yu Li-Fang
Published: November 2019

Previously we identified a series of amidoalkylindoles as potent and selective CB2 partial agonists.
A hybrid ionic liquid-based electrolyte for high-performance lithium–sulfur batteries
Authors: Lu Hai , Zhu Yan , Zheng Bin , Du Huiling , Zheng Xuezhao , Liu Changchun , Yuan Yan , Fang Jing , Zhang Kai
Published: November 2019

A hybrid IL-based electrolyte consisting of P1,2O1TFSI and the support solvent(s) of DOL and/or ETFE was applied in Li–S batteries, and a rational balance between Li2Sx dissolution and Li protection to achieve controllable shuttle was proposed.
Recyclable bimetallic CuMoO4 nanoparticles for C–N cross-coupling reaction under mild conditions
Authors: Panigrahi Reba , Panda Subhalaxmi , Behera Pradyota Kumar , Sahu Santosh Kumar , Rout Laxmidhar
Published: November 2019

New bimetallic CuMoO4 nanocatalysts for C–N cross-coupling.
Distinctively complete inhibition of fibrillation of serum albumins by methotrexate in vitro: experimental and modelling studies to understand the tuning of protein misfolding-related aggregations
Authors: Khatua Deb Kumar , Halder Mintu
Published: November 2019

Formation of amyloid fibril and non-fibril agglomerations of BSA in the absence and presence of FA and MTX, respectively.
Surface roughness influences the protein corona formation of glycosylated nanoparticles and alter their cellular uptake
Authors: Piloni Alberto , Wong Chin Ken , Chen Fan , Lord Megan , Walther Andreas , Stenzel Martina H.
Published: November 2019

Patterned nanoparticle surfaces can repel protein absorption and prevent the formation of a protein corona, which alters the biological behavior and therefore the fate of the nanoparticle.
Cobalt-doping SnS2 nanosheets towards high-performance anodes for sodium ion batteries
Authors: Wang Liqin , Zhao Quanqing , Wang Zhitao , Wu Yujun , Ma Xilan , Zhu Youqi , Cao Chuanbao
Published: November 2019

The stable 3D integral electrode structure and the heteroatom-doping effect of Sn0.9Co0.1S2/CC nanosheets lead to outstanding electrochemical properties for sodium ion batteries.
Label-free and enzyme-free detection of microRNA based on a hybridization chain reaction with hemin/G-quadruplex enzymatic catalysis-induced MoS2 quantum dots via the inner filter effect
Authors: Ge Jia , Qi Zhangyu , Zhang Liangliang , Shen Xueping , Shen Yanmei , Wang Weixia , Li Zhaohui
Published: November 2019

A new label-free, enzyme-free, and specific strategy for the assay of microRNA has been developed based on a hybridization chain reaction with hemin/G-quadruplex enzymatic catalysis-induced MoS2 quantum dots via the inner filter effect.
Aqueous stable Pd nanoparticles/covalent organic framework nanocomposite: an efficient nanoenzyme for colorimetric detection and multicolor imaging of cancer cells
Authors: Sun Panpan , Hai Jun , Sun Shihao , Lu Siyu , Liu Sha , Liu Hanwen , Chen Fengjuan , Wang Baodui
Published: November 2019

We have prepared an aqueous stable folic acid functionalized Pd NPs/CMC-COF-LZU1 nanoenzyme for precise, sensitive, quantitative and rapid detection of cancer cells.
2D material broadband photodetectors
Authors: Yao Jiandong , Yang Guowei
Published: November 2019

2D materials and their hybrid structures have high application prospects in broadband photodetection, making them promising complements to traditional schemes.
Synthesis of poly[oligo(hexafluoropropylene oxide) perfluoroisopropenylether (PIPE)] graft copolymers with vinylidene fluoride (VDF) using CF3radicals
Authors: Burgess Trevor J. , Vitale Alessandra , Joly-Duhamel Christine , Bongiovanni Roberta , Manseri Abdelatif , Ono Taizo , Améduri Bruno , Friesen Chadron M.
Published: November 2019

The synthesis of poly(vinylidene fluoride)-graft-oligomeric hexafluoropropylene oxide copolymers [poly[VDF-g-oligo(HFPO)-PIPE] is provided, having glass transitions ofca.−82 °C and respective water and hexadecane contact angles ofca.133° and 70°.
Synthesis of selenide-containing polymers by multicomponent polymerization based on γ-butyroselenolactone
Authors: Chen Sisi , Pan Xiangqiang , Zhu Jian , Zhu Xiulin
Published: November 2019

A versatile protocol for the synthesis of various multiresponsive selenide-containing polymeric architectures was developed by multicomponent polymerization (MCP) of primary diamines, γ-butyroselenolactone and electrophilic reagents.
Ni-Catalyzed electrophile driven regioselective arylative cyclization of ortho-functional diaryl acetylenes for the synthesis of pyridine and indene derivatives
Authors: Singam Maneesh Kumar Reddy , Nagireddy Attunuri , Rajesh Manda , Ganesh Veeramalla , Reddy Maddi Sridhar
Published: November 2019

A regioselective arylative cyclization of ortho functional diaryl acetylenes for the synthesis of selectively substituted diaryl pyridine and indene derivatives is accomplished through an electrophile driven alkyne polarization.
Green synthesis of zinc oxide nanostructures and investigation of their photocatalytic and bactericidal applications
Authors: Kahsay Mebrahtu Hagos , Tadesse Aschalew , RamaDevi Dharamasoth , Belachew Neway , Basavaiah K.
Published: November 2019

We report a facile one-pot green synthesis of zinc oxide (ZnO) nanostructures using aqueous leaf extract of Dolichos Lablab L. as the reducing and capping agent.
Constrained Bayesian optimization for automatic chemical design using variational autoencoders
Authors: Griffiths Ryan-Rhys , Hernández-Lobato José Miguel
Published: November 2019

Automatic Chemical Design is a framework for generating novel molecules with optimized properties.
Detection of key transient Cu intermediates in SSZ-13 during NH3-SCR deNOx by modulation excitation IR spectroscopy
Authors: Greenaway Alex G. , Marberger Adrian , Thetford Adam , Lezcano-González Inés , Agote-Arán Miren , Nachtegaal Maarten , Ferri Davide , Kröcher Oliver , Catlow C. Richard A. , Beale Andrew M.
Published: November 2019

Protagonists in the standard SCR reaction have been caught in the act by modulation excitation IR & XAFS spectroscopy.
Disruption of microtubule function in cultured human cells by a cytotoxic ruthenium(ii) polypyridyl complex
Authors: Alatrash Nagham , Issa Faiza H. , Bawazir Nada S. , West Savannah J. , Van Manen-Brush Kathleen E. , Shelor Charles P. , Dayoub Adam S. , Myers Kenneth A. , Janetopoulos Christopher , Lewis Edwin A. , MacDonnell Frederick M.
Published: November 2019

Treatment of cultured human cell lines with a cytotoxic IC50dose of ∼2 μM tris(diphenylphenanthroline)ruthenium(ii) chloride (RPC2) retards or arrests microtubule motion as tracked by visualizing fluorescently-tagged microtubule plus end-tracking proteins.
Correction: Theory for polariton-assisted remote energy transfer
Authors: Du Matthew , Martínez-Martínez Luis A. , Ribeiro Raphael F. , Hu Zixuan , Menon Vinod M. , Yuen-Zhou Joel
Published: November 2019

Correction for ‘Theory for polariton-assisted remote energy transfer’ by Matthew Du et al., Chem. Sci., 2018, 9, 6659–6669.
Correction: Polariton chemistry: controlling molecular dynamics with optical cavities
Authors: Ribeiro Raphael F. , Martínez-Martínez Luis A. , Du Matthew , Campos-Gonzalez-Angulo Jorge , Yuen-Zhou Joel
Published: November 2019

Correction for ‘Polariton chemistry: controlling molecular dynamics with optical cavities’ by Raphael F. Ribeiro, et al., Chem. Sci., 2018, 9, 6325–6339.
Correction: Impact of perovskite solar cell degradation on the lifetime energy yield and economic viability of perovskite/silicon tandem modules
Authors: Qian Jiadong , Ernst Marco , Wu Nandi , Blakers Andrew
Published: November 2019

Correction for ‘Impact of perovskite solar cell degradation on the lifetime energy yield and economic viability of perovskite/silicon tandem modules’ by Jiadong Qian et al., Sustainable Energy Fuels, 2019, 3, 1439–1447.
Fluctuations and conformational stability of a membrane patch with curvature inducing inclusions
Authors: Pezeshkian Weria , Ipsen John H.
Published: November 2019

Membranes with curvature inducing inclusions display a range of cooperative phenomena, which can be linked to biomembrane function, e.g. membrane tubulation, vesiculation, softening and spontaneous tension.
One-pot synthesis of oligonucleotide-grafted polymeric nanoparticles
Authors: Haladjova Emi , Ugrinova Iva , Rangelov Stanislav
Published: November 2019

Novel polymeric SNAs, preparedviaan original synthetic approach, undergo reversible changes in the grafting density and conformation of the oligonucleotide strands.
Molecular transport in systems containing binding obstacles
Authors: Polanowski Piotr , Sikorski Andrzej
Published: November 2019

We studied the movement of particles in crowded environments by means of extensive Monte Carlo simulations. The detailed analysis of the influence of binding obstacles on motion of the particles was performed.
Disulfide chemistry in responsive aggregation of amphiphilic systems
Authors: Bej Raju , Dey Pradip , Ghosh Suhrit
Published: November 2019

We highlight the nanostructure assemblies containing disulfide linkages, dynamics, GSH triggered disassembly and implications in biological applications.
Effective oxygen reduction on A-site substituted LaCuO3−δ: toward air electrodes for SOFCs based on perovskite-type copper oxides
Authors: Niemczyk Anna , Du Zhihong , Olszewska Anna , Marzec Mateusz , Gajewska Marta , Świerczek Konrad , Zhao Hailei , Poudel Bisham , Dabrowski Bogdan
Published: November 2019

It is shown that appropriately doped Cu-based perovskite-type oxides exhibit high catalytic activity toward the oxygen reduction reaction at high temperatures, making them suitable candidates for the air electrodes for solid oxide fuel cells.
A thermo-sensitive injectable hydroxypropyl chitin hydrogel for sustained salmon calcitonin release with enhanced osteogenesis and hypocalcemic effects
Authors: Yu Peng , Xie Jing , Chen Yu , Liu Jinming , Liu Yanpeng , Bi Bo , Luo Jun , Li Sheyu , Jiang Xulin , Li Jianshu
Published: November 2019

An injectable thermo-sensitive hydroxypropyl chitin hydrogel was applied for long-term sCT release, with enhanced osteogenic potential and hypocalcemic effects.
Significant enhancement in the thermoelectric performance of Bi2O2S through dimensionality reduction
Authors: Zhang Renqi , Zhou Zizhen , Qi Ning , Zhao Bin , Zhang Quankun , Zhang Ziye , Chen Zhiquan
Published: November 2019

The thermoelectric conversion efficiency η in the Bi2O2S monolayer (bilayer) has been enhanced by 75% (42%) through dimensionality reduction.
Astaxanthin reduces perfluorooctanoic acid cytotoxicity in Saccharomyces cerevisiae
Authors: Sudharshan S. J. , Tirupathi Raghavendra , Dyavaiah Madhu
Published: November 2019

Perfluorooctanoic acid (PFA) has been identified as an environmental contaminant of high concern for human health. In this study, we demonstrated that PFA induces a dose (0 to 1.5 mM) dependent cytotoxicity in S. cerevisiae cells which can be rescued by astaxanthin. The percent sensitivity induced by PFA and the cell protection offered by astaxanthin (30 μM) were demonstrated by CFU counts and spots. The increase in intracellular ROS, superoxide dismutase (SOD), glutathione and lipid peroxidation levels in PFA treated cells suggested that increased oxidative stress resulted in yeast cell death. In contrast, decreased ROS level, increased SOD activity, reduced glutathione and decreased lipid peroxidation by astaxanthin supplementation suggest that the cells are protected from the PFA induced oxidative stress mediated cytotoxicity. Reduced chromatin condensation and nuclear fragmentation in astaxanthin pre-treated cells indicate that astaxanthin rescued the cells from PFA induced apoptosis. Our overall results suggest that PFA induces oxidative stress-mediated cytotoxicity in yeast cells, which were rescued by astaxanthin treatment.
Astilbin attenuates cerebral ischemia/reperfusion injury by inhibiting the TLR4/MyD88/NF-κB pathway
Authors: Li Jing , Gu Zhaowei , Liu Yue , Wang Yu , Zhao Min
Published: November 2019

Ischemic stroke is the second most common cause of death worldwide and cerebral ischemia/reperfusion (I/R) injury also leads to serious tissue damage. Astilbin, a natural bioactive flavonoid compound, has been reported to have protective effects on neurological diseases. This study aims to investigate the effects of astilbin on cerebral I/R injury and determine the mechanisms involved. The results demonstrated that, in cerebral I/R rats, astilbin could attenuate I/R injury in the hippocampal region, decreasing the activity of lactate dehydrogenase (LDH) and malondialdehyde (MDA) in the rat brain. Astilbin also inhibited the I/R-induced upregulation of pro-inflammatory mediators (TNFα, IL-1β, IL-6). Similarly, in hypoxia/reperfusion (H/R) treated human neuroblastoma cells, astilbin could increase the cell viability of SH-SY5Y, decrease the activity of LDH and MDA, and inhibit the H/R-induced upregulation of pro-inflammatory mediators. For the mechanism study, western blot results indicated that astilbin could inhibit the expression of Toll-like receptor 4 (TLR4), myeloid differential protein 88 (MYD88) and phosphorylated NF-κB p65 in H/R treated SH-SY5Y cells. The research indicated that astilbin ameliorated cerebral I/R injury partly via the TLR4/MyD88/NF-κB pathway. Astilbin may have potential therapeutic effects on cerebral ischemia.
Biphenyl degradation by recombinant photosynthetic cyanobacteriumSynechocystissp. PCC6803 in an oligotrophic environment using unphysiological electron transfer
Authors: Suzuki Takaaki , Nishizawa Akito , Kikuchi Masashi , Nonaka Chihiro , Komuro Mariko , Nakayama Miki , Kashino Yasuhiro , Fukuda Masao , Kimura Shigenobu
Published: November 2019

Cyanobacteria are potentially useful photosynthetic microorganisms for bioremediation under oligotrophic environments. Here, the biphenyl degradation pathway genes of β-proteobacterium Acidovorax sp. strain KKS102 were co-expressed in cyanobacterium Synechocystis sp. PCC6803 cells under control of the photo-inducible psbE promoter. In the KKS102 cells, biphenyl is dioxygenated by bphA1 and bphA2 gene products complex using electrons supplied from NADH via bphA4 and bphA3 gene products (BphA4 and BphA3, respectively), and converted to benzoic acid by bphB, bphC and bphD gene products. Unexpectedly, biphenyl was effectively hydroxylated in oligotrophic BG11 medium by co-expressing the bphA3, bphA1 and bphA2 genes without the bphA4 gene, suggesting that endogenous cyanobacteria-derived protein(s) can supply electrons to reduce BphA3 at the start of the biphenyl degradation pathway. Furthermore, biphenyl was converted to benzoic acid by cyanobacterial cells co-expressing bphA3, bphA1, bphA2, bphB, bphC and bphD. Structural gene-screening using recombinant Escherichia coli cells co-expressing bphA3, bphA1, bphA2, bphB and bphC suggested that petH, which encodes long- and short-type NADP-ferredoxin oxidoreductase isomers (FNRL and FNRS, respectively), and slr0600, which is annotated as an NADPH-thioredoxin reductase gene in CyanoBase, were BphA3-reducible proteins. Purified FNRL and FNRS, and the slr0600 gene product showed BphA3 reductase activity dependent on NADPH and the reduced form of glutathione, respectively, potentially shedding light on the physiological roles of the slr0600 gene product in cyanobacterial cells. Collectively, our results demonstrate the utility of Synechocystis sp. PCC6803 cells as a host for bioremediation of biphenyl compounds under oligotrophic environments without an organic carbon source.
MicroRNA-15a modulates lens epithelial cells apoptosis and proliferation through targeting B-cell lymphoma-2 and E2F transcription factor 3 in age-related cataracts
Authors: Li Qiao , Pan HaiTao , Liu QingHuai
Published: November 2019

Age-related cataract remains a serious problem in the aged over the world. MicroRNAs are abnormally expressed in various diseases including age-related cataract. MicroRNA-15a (MicroRNA-15a) has been involved in various diseases and plays crucial roles in many cellular processes. However, the mechanism of microRNA-15a in the genesis of cataract remains barely known. We therefore aimed to investigate the role of microRNA-15a in the cataract. Herein, human lens epithelial B3 cells, HLE-B3 cells were treated with 200 μmol/l H2O2 for 24 h. H2O2 was utilized in our study to induce HLE-B3 cells injury. We observed that cell apoptosis was induced by the treatment of H2O2 and meanwhile, cell proliferation was repressed by 200 μmol/l H2O2. Then, it was found that microRNA-15a was significantly increased with the H2O2 exposure in vitro. Importantly, B-cell lymphoma-2 (BCL2) and E2F transcription factor 3 (E2F3) exert crucial roles in cell apoptosis and cell proliferation. We found that BCL2 and E2F3 were greatly reduced by 200 μmol/l H2O2 in human lens epithelial cells. In addition, microRNA-15a overexpression induced cell apoptosis and repressed cell proliferation through suppressing BCL2 and E2F3. Subsequently, BCL2 and E2F3 were predicted as a direct target of microRNA-15a. The direct correlation between microRNA-15a and BCL2/E2F3 was confirmed by dual luciferase reporter assay. In conclusion, we demonstrated that microRNA-15a triggered apoptosis and repressed the proliferation of HLE-B3 cells by modulating BCL2 and E2F3.
The Relationship Between Speech Perceptual Discrimination and Speech Production in Parkinson's Disease
Authors: Mollaei Fatemeh , Shiller Douglas M. , Baum Shari R. , Gracco Vincent L.
Published: November 2019

Purpose

We recently demonstrated that individuals with Parkinson's disease (PD) respond differentially to specific altered auditory feedback parameters during speech production. Participants with PD respond more robustly to pitch and less robustly to formant manipulations compared to control participants. In this study, we investigated whether differences in perceptual processing may in part underlie these compensatory differences in speech production.

Methods

Pitch and formant feedback manipulations were presented under 2 conditions: production and listening. In the production condition, 15 participants with PD and 15 age- and gender-matched healthy control participants judged whether their own speech output was manipulated in real time. During the listening task, participants judged whether paired tokens of their previously recorded speech samples were the same or different.

Results

Under listening, 1st formant manipulation discrimination was significantly reduced for the PD group compared to the control group. There was a trend toward better discrimination of pitch in the PD group, but the group difference was not significant. Under the production condition, the ability of participants with PD to identify pitch manipulations was greater than that of the controls.

Conclusion

The findings suggest perceptual processing differences associated with acoustic parameters of fundamental frequency and 1st formant perturbations in PD. These findings extend our previous results, indicating that different patterns of compensation to pitch and 1st formant shifts may reflect a combination of sensory and motor mechanisms that are differentially influenced by basal ganglia dysfunction.
VIS3COS
Authors: Paulino-Afonso Ana , Sobral David , Darvish Behnam , Ribeiro Bruno , Smail Ian , Best Philip , Stroe Andra , Cairns Joseph
Published: November 2019

We present spectroscopic observations of 466 galaxies in and around a superstructure at z ∼ 0.84 targeted by the VIMOS Spectroscopic Survey of a Supercluster in the COSMOS field (VIS3COS). We use [OII]λ3727, Hδ, and Dn4000 to trace recent, medium-, and long-term star formation histories and investigate the effect of stellar mass and local environment on them. By studying trends in individual and composite galaxy spectra, we find that stellar mass and environment play a role in the observed galactic properties. Galaxies with low stellar mass (10 <  log10(M⋆/M⊙) < 10.5) in the field show the strongest Hδ absorption. Similarly, the massive population (log10(M⋆/M⊙) > 11) shows an increase in Hδ absorption strengths in intermediate-density environments (e.g. filaments). Galaxies with intermediate stellar mass (10.5 <  log10(M⋆/M⊙) < 11) have similar Hδ absorption profiles in all environments, but show an indication of enhanced [OII] emission in intermediate-density environments. This indicates that field galaxies with low stellar mass and filament galaxies with high stellar mass are more likely to have experienced a recent burst of star formation, while galaxies of the intermediate stellar-mass show an increase of star formation at filament-like densities. We also find that the median [OII] equivalent width (|EW[OII]|) decreases from 27 ± 2 Å to 2.0+0.5−0.4 Å and Dn4000 increases from 1.09 ± 0.01 to 1.56 ± 0.03 with increasing stellar mass (from ∼109.25 to ∼1011.35 M⊙). For the dependence on the environment, we find that at fixed stellar mass, |EW[OII]| is tentatively lower in environments with higher density. We find for Dn4000 that the increase with stellar mass is sharper in denser environments, which indicates that these environments may accelerate galaxy evolution. Moreover, we find higher Dn4000 values in denser environments at fixed stellar mass, suggesting that galaxies are on average older and/or more metal rich in these dense environments. This set of tracers depicts a scenario where the most massive galaxies have, on average, the lowest specific star formation rates and the oldest stellar populations (age ≳ 1 Gyr, showing a mass-downsizing effect). We also hypothesize that the observed increase in star formation (higher EW[OII]|, higher specific star formation rate) at intermediate densities may lead to quenching because we find that the quenched fraction increases sharply from the filament to cluster-like regions at similar stellar masses.
New predictions for radiation-driven, steady-state mass-loss and wind-momentum from hot, massive stars
Authors: Sundqvist J. O. , Björklund R. , Puls J. , Najarro F.
Published: November 2019

Context. Radiation-driven mass loss plays a key role in the life cycles of massive stars. However, basic predictions of such mass loss still suffer from significant quantitative uncertainties.

Aims. We develop new radiation-driven, steady-state wind models for massive stars with hot surfaces, suitable for quantitative predictions of global parameters like mass-loss and wind-momentum rates.

Methods. The simulations presented here are based on a self-consistent, iterative grid solution to the spherically symmetric, steady-state equation of motion, using full non-local thermodynamic equilibrium radiative transfer solutions in the co-moving frame to derive the radiative acceleration. We do not rely on any distribution functions or parametrization for computation of the line force responsible for the wind driving. The models start deep in the subsonic and optically thick atmosphere and extend up to a large radius at which the terminal wind speed has been reached.

Results. In this first paper, we present models representing two prototypical O-stars in the Galaxy, one with a higher stellar mass M*∕M⊙ = 59 and luminosity log10L*∕L⊙ = 5.87 (spectroscopically an early O supergiant) and one with a lower M*∕M⊙ = 27 and log10L*∕L⊙ = 5.1 (a late O dwarf). For these simulations, basic predictions for global mass-loss rates, velocity laws, and wind momentum are given, and the influence from additional parameters like wind clumping and microturbulent speeds is discussed. A key result is that although our mass-loss rates agree rather well with alternative models using co-moving frame radiative transfer, they are significantly lower than those predicted by the mass-loss recipes normally included in models of massive-star evolution.

Conclusions. Our results support previous suggestions that Galactic O-star mass-loss rates may be overestimated in present-day stellar evolution models, and that new rates might therefore be needed. Indeed, future papers in this series will incorporate our new models into such simulations of stellar evolution, extending the very first simulations presented here toward larger grids covering a range of metallicities, B supergiants across the bistability jump, and possibly also Wolf-Rayet stars.
Methods of increasing the efficiency of the worm gear
Authors: Melnikov Pavel , Schegoleva Svetlana
Published: November 2019

This article describes the geometric features of the worm gear, the principle of operation and the theoretical basis for calculating the efficiency. A number of research works aimed at the development of new advanced techniques, both production and use of worm gear were analyzed. Based on the analyzed techniques, one was chosen and confirmed experimentally. Based on the performed researches and experiments, recommendations were developed to improve the efficiency of the worm gear type 2H-63.
Diagnosing of automotive alternators on thermal state
Authors: Puzakov Andrey
Published: November 2019

Since automotive alternators serve as the primary sources of power onboard of vehicles, the online diagnostics of technical conditions thereof is a relevant task. An advantage of temperature as a diagnostic parameter is sensitivity to most faults at the early stage of their development. Physical modeling of faults (stator one-line open fault, stator turn-to-turn short circuit, stator winding phase-to-phase short circuit, circuit opening and short circuit of rectifier diodes) has been done by forced increase (decrease) of electrical resistance between alternator elements. In order to measure alternator temperature, it has been brought to steady thermal state within 20 minutes. It has been found that the alternator temperature in case of faults can increase the rated temperature by 10-30 °С even when the alternator operates without load. An algorithm has been developed to find alternator faults by evaluating the thermal state thereof, which can become a basis of an onboard automatic online diagnosing system of an automotive alternator.
Increasing the operational life of the cutting tool based on stress relieving
Authors: Chuikov R.S. , Chuikov S.S. , Stavyshenko A.S. , Samohvalov V.D.
Published: November 2019

The paper covers the results of studying the influence of internal stresses arising in the manufacture of changeable polygonal plates of TC type hard alloys on the resistance of the cutting tool and the possibility of relieving them by preheating the cutting plates before cutting. A method and a special design are proposed for preheating changeable polygonal plates in order to relieve internal stresses in tool hard alloys and increase the resistance of the cutting tool.
Mathematical modelling and optimization of the electroplating process with a rotating cathode to reduce the non-uniformity of the coating thickness
Authors: Solovjev Denis , Solovjeva Inna , Konkina Viktoriya
Published: November 2019

The article is devoted to the development of an approach to improving the electroplating uniformity on the cathode surface, rotating around its axis, using a figured anode. A mathematical model of a stationary process with distributed coordinates, based on the equations of theoretical electrochemistry, has been developed for this approach. The problem for optimizing the anode shape according to the criterion for minimizing the non-uniformity of the deposited coating on the rotating cathode is formulated. The results of solving this problem are demonstrated on the example of the Zinc plating. Possible improvements to improve the accuracy of the calculation results for the developed mathematical model are given in the conclusion of the article.
Thermal properties of products based on ABS / PC
Authors: Romanova Natalia , Shafigullin Lenar , Gabdrakhmanov Azat , Buyatova Svetlana
Published: November 2019

Polymer blends are capable of providing materials with extended useful properties beyond the range that can be obtained from single polymers. Acrylonitrile-butadiene-styrene and polycarbonate blends are important industrial materials, especially, in the machine building industry, as ABS improves the processing properties of parts and their toughness, and they also feature positive thermodynamic interaction between PC and styrene-acrylonitrile (SAN) copolymer as a part of ABS. DSC method was used to study the thermal properties of ABS and ABS/PC blends with various ratios. The result from this work shows that abnormal endothermic effect in the ABS glass transition region implies an inadequate manufacturing process for automotive components. The paper studied the influence of ABS/PC blend ratios on the value of glass transition temperature and delta Cp, and it was found that the Tg value for SAN/PC increased with decreasing ABS for ABS/PC blends with mass ratio of 75/25, 60/40, 50/50.
Structural and dynamic characteristics of technological power unit for heat treatment of vermiculite concentrates
Authors: Nizhegorodov Anatolij , Gavrilin Alexey , Moyzes Boris
Published: November 2019

The considered electric furnace with a mobile base is the most innovative power unit providing energy consumption of 48…52 mJ/m3during the process of vermiculite firing which has never been achieved before. New power unit was developed for all mechanical shortcomings of the test prototype were eliminated. By eliminating the resonating base plate and switching to the platform with forced excitation of its vibrations, the influence of fluctuations in the excitation frequency of the oscillatory system on its technological parameters, ensuring the duration of vermiculite heat treatment, has been eliminated. The activity of high sensitivity of the oscillations of the base platform to the change in friction, which appears during operation, is also completely removed. In addition, the problem of a controlled and regulated vibrational method of supplying raw materials to the furnace without the use of drum doses has been solved. It simplifies the design and cost of new power units, which are characterized by doubled productivity and dynamic balance.
Regulation of the cooling fan rotation speed, as an effective instrument for reducing mechanical consumption of energy
Authors: Lomakin George , Kondakov Sergey , Kochnev Ilya
Published: November 2019

Drawing on the example of engine unit YaMZ-652, by means of calculation, and theoretically, the efficiency of using stepless regulation of the cooling fan rotation speed. The greatest effect from the regulation is achieved in the range of small and average loads of the engine performance at the regimes being tested.
Features of calculation of steel structures of bridge cranes at variable loads
Authors: Akhtulov A. L. , Kirasirov O. M. , Kirasirov M. O.
Published: November 2019

The article deals with the calculation of metal bridge cranes operating under the influence of variable loads. Requirements to static and dynamic characteristics of mechanisms of load-lifting cranes are caused by specifics of work, features of a design and operating conditions of the cranes working at variable loadings. Thus, it is proposed to perform calculations of parts of crane mechanisms for endurance, operating under non-stationary variable load, based on the principle of linear summation of damage, allowing the calculation from the point of view of the load equivalent to the entire range of operating loads.