bluuebunny/crossref_metadata_2025 huggingface dataset:
Attitudes towards hearing difficulties, health-seeking behaviour, and hearing aid use among older adults in Thailand
Authors: Pornprasit Panicha , Utoomprurkporn Nattawan , Areekit Poonsub , Manum Pornnapat , Thanutammakul Sutatta , Parmar Bhavisha , Adamson Joy , Bamiou Doris
Published: January 2024

Introduction

This qualitative study explores older adults' attitudes towards hearing difficulties, health-seeking behaviour and hearing aid use in Samutprakarn, Thailand.

Method

Three focus groups (n=25), with adults aged 60-88 years, were conducted within a residential complex for older adults. Qualitative data analysis, employing a phenomenological approach was completed.

Results

Four themes are presented: (1) Perception of hearing difficulties, (2) Experience of receiving care, (3) Attitudes towards wearing hearing aids, and (4) Raising awareness of hearing difficulties in older adults. Findings indicate that Thai older adults may not be aware or accepting of their hearing difficulties, due to the stigma associated hearing loss, older age, and disability. The consequential impact of these unacknowledged issues manifest in communication challenges and the adoption of avoidance behaviours across diverse situational contexts.

Conclusion

This study enhances our understanding of how Thai older adults perceive hearing problems and ear care. Findings underscore the potential pivotal role of familial and social networks in mitigating barriers associated with hearing difficulties among older adults in Thailand. These insights can raise awareness and inform effective management for older adults and their families. Additionally, the findings could guide healthcare providers, researchers, and policy makers, fostering improved care for this demographic.
Lipid accumulation product is an effective predictor of metabolic syndrome in non-obese women with polycystic ovary syndrome
Authors: Han Wenju , Zhang Meiwei , Wang Haiyan , Yang Yitian , Wang Lei
Published: January 2024

Objective

To explore the correlation of lipid accumulation product (LAP) with metabolic syndrome (MS) and to assess the predictive value of LAP for MS risk in polycystic ovary syndrome (PCOS) with different body mass index (BMI).

Methods

A total of 242 PCOS patients and 150 controls were recruited and divided into normal-weight, overweight, and obese groups, then further divided into MS and without MS subgroups. Clinical and anthropometric variables and laboratory results were recorded. LAP was calculated from waist circumference (WC) and triglyceride using sex-specific formulae. Logistic regression analysis and receiver operating characteristic (ROC) curve were applied to determine and analyze the predictive value of LAP for MS.

Results

The prevalence of MS among PCOS patients was 45.04%, which was significantly higher than that of the controls (10%). Stratified by BMI, the incidence of MS in the normal-weight, overweight, and obese PCOS groups were 15.58%, 41.43%, and 71.58%, respectively. Logistic regression analysis indicated that LAP was an independent risk factor for MS in both normal-weight and overweight groups; however, the results were not significant in the obese group. ROC curve analysis showed that LAP had an outstanding discrimination index for MS in normal-weight (AUC=0.960, cut-off value=42.5) and overweight (AUC=0.937, cut-off value=47.93) PCOS patients, with a sensitivity of 0.917/0.931 (normal-weight/overweight) and a specificity of 0.969/0.854 (normal-weight/overweight), respectively.

Conclusion

Normal-weight and overweight PCOS patients also have a fairly high incidence of MS and should receive as much attention as obese patients. Compared to applying multiple clinical indicators, LAP is more convenient and facilitates acquiring early and accurate diagnoses of MS among non-obese PCOS patients using fewer MS markers.
Gut microbiota and metabolites exhibit different profiles after very-low-caloric restriction in patients with type 2 diabetes
Authors: Gong Tong , Di Hongjie , Hu Yongxin , Xu Shuhang , Chen Jie , Chen Guofang , Wei Xiao , Liu Chao
Published: January 2024

Background and aims

To investigate the effect of short-term very-low-calorie restriction (VLCR) on metabolism in patients with type 2 diabetes (T2D), and elucidate the molecular mechanism through analyses on gut microbiota and small-molecule metabolites.

Methods

Fourteen T2D patients were hospitalized to receive VLCR (300-600 kcal/d) for 9 days. BMI, BP, and HR were taken before and after VLCR. Levels of blood lipids, fasting insulin, FBG, and 2h PBG were assessed. The microbial diversity in feces was detected by 16S rDNA high-throughput sequencing technology, and small-molecule metabolites in plasma and feces by untargeted metabolomics technology.

Results

After VLCR, BW, BMI, WC, BP, and levels of FBG and 2h PBG, insulin, HOMA-IR, and triglyceride decreased significantly in T2D patients (P<0.05). There was no significant change in the α-diversity of fecal microbiota, but the abundance of Bacteroidetes increased significantly, and the Firmicutes/Bacteroidetes ratio decreased significantly from 11.79 to 4.20. Parabacteroides distasonis showed an abundance having increased most prominently after VLCR treatment. Plasma level of amino acid metabolite L-arginine increased significantly. Plasma levels of three lipid metabolites, PC (14:0/20:4 [8Z, 11Z, 14Z, 17Z]), LysoPC (16:1 [9Z]) and LysoPC (18:1 [11Z]), were significantly reduced. Fecal levels of lipid metabolite LysoPC (18:1 [11Z]) and bile acid metabolite glycholic acid were significantly decreased.

Conclusion

In T2DM patients, VLCR can considerably reduce body weight and improve glucose and lipid metabolism without causing severe side effects. LysoPC (18:1 [11Z]) and Parabacteroides distasonis showed the most obvious difference after VLCR, which could be the indicators for VLCR in T2D.
Application and discoveries of metabolomics and proteomics in the study of female infertility
Authors: Shi Junhua , Wu Xingjie , Qi Haiou , Xu Xin , Hong Shihao
Published: January 2024

Introduction

Female infertility is defined as the absence of clinical pregnancy after 12 months of regular unprotected sexual intercourse.

Methods

This study employed metabolomics and proteomics approaches to investigate the relationship between metabolites and proteins and female infertility. The study used metabolomics and proteomics data from the UK Biobank to identify metabolites and proteins linked to infertility.

Results

The results showed that GRAM domain-containing protein 1C and metabolites fibrinogen cleavage peptides ADpSGEGDFXAEGGGVR and 3-Hydroxybutyrate had a positive correlation with infertility, whereas proteins such as Interleukin-3 receptor subunit alpha, Thrombospondin type-1 domain-containing protein 1, Intestinal-type alkaline phosphatase, and platelet and endothelial cell adhesion molecule 1 exhibited a negative correlation. These findings provide new clues and targets for infertility diagnosis and treatment. However, further research is required to validate these results and gain a deeper understanding of the specific roles of these metabolites and proteins in infertility pathogenesis.

Discussion

In conclusion, metabolomics and proteomics techniques have significant application value in the study of infertility, allowing for a better understanding of the biological mechanisms underlying infertility and providing new insights and strategies for its diagnosis and treatment. These research findings provide a crucial biological mechanistic basis for early infertility screening, prevention, and treatment.
Reliability-constrained capacity market design with high proportions of renewable energies
Authors: Zheng Chen , Nan Shang
Published: January 2024

Renewable energy generation (REG) has continued to grow strongly and resulted in the lower profitability of conventional generation. Meanwhile, the indeterminacy of REG will also lead to electricity balancing challenges and more volatile and less-predictable physical flows in the power grid. In this case, a capacity market is essential to motivate new investments and, therefore, ensure supply adequacy. Considering the low-carbon transition goal and the requirements of the reliability of the power system, this paper develops a reliability-constrained capacity market framework in which the reliability criterion rather than the capacity supply–demand equilibrium is taken into account. The ramping constraints, devices’ random failures, and REG uncertainties are comprehensively considered in the capacity requirement determination and allocation. First, a comprehensive capacity market mechanism coordinated with multi-objective regulations is proposed to compensate the capacity providers and encourage the renewable energy transition. Then, a novel capacity market model is proposed to clear the market with reliability constraints. Moreover, to reduce the computational burden caused by the explicit consideration of reliability constraints, several techniques are applied including the root-event-based state screening technique and the adaptive Kriging metamodel. A modified IEEE-RTS-79 case is studied to illustrate the benefits of the proposed reliability-constrained capacity market model.
Research progress on animal habitat constructions from the perspective of urban biodiversity improvement
Authors: Liu Zhiruo , Yin Hao , Wang Yang , Cheng Qiushuang , Wang Zihan
Published: January 2024

The accelerated urbanization process has caused problems such as habitat loss, isolation, and habitat quality decline, resulting in a sharp reduction in the richness and abundance of urban species. Constructing suitable habitat environmental conditions is the most direct and effective way to protect animals. In urban environment, habitat construction can be achieved by integrating species protection and landscape planning, which is also an important manifestation of biodiversity conservation at the ecosystem level. Understanding how to incorporate animal habitats into city plan and design is critical and urban planners would benefit from a review that holistically describes the steps and methods of animal habitat constructions. We conducted a review to highlight the animal habitats space resources and network structures. We synthesized the findings of research studies in the last 20 years to illuminate the investigation, assessment, planning and management of animal habitats. As habitat degradation and fragmentation in anthropogenic environments, our findings suggest city planners should consider ecological background investigation, habitat suitability assessment, habitat planning strategies and animal habitat management as four key steps of mitigation to alleviate these impacts. This study will provide a useful reference to improve animal survival quality and communication. Through this study, the consolidated research can aid in sustainable development and innovation to promote the ecological function of urban green space and the harmonious coexistence of humans and animals.
Estimation of hourly one square kilometer fine particulate matter concentration over Thailand using aerosol optical depth
Authors: Punpukdee Pongsakon , Winijkul Ekbordin , Kyaw Pyae Phyo , Virdis Salvatore G. P. , Xue Wenchao , Nguyen Thi Phuoc Lai
Published: January 2024

In the recent years, concentration of fine particulate matter that are 2.5 microns or less in diameter (PM2.5) in Thailand has consistently exceeded the national ambient air quality standard. Currently, the measurement of PM2.5 concentration relies on air quality monitoring stations operated by the Pollution Control Department of Thailand (PCD). However, these stations are insufficient, particularly in rural areas, where agricultural open burning are major sources of pollution after harvesting period. This study aims to enhance the monitoring of PM2.5 concentration by leveraging cost-effective technologies. We propose the integration of satellite data, specifically Aerosol Optical Depth (AOD) from Multi-Angle Atmospheric Correction (MAIAC) product and Himawari-8 satellites, with the Weather Research and Forecasting Model (WRF) data, to provide supplementary data to the ground-based monitoring. Hourly 5 × 5 km2 AOD data from Himawari-8 were downscaled to a high-resolution of 1 × 1 km2, leveraging the AOD distribution pattern of the concurrent MAIAC product using eXtreme Gradient Boosting (XGBoost) model. Notably, during Thailand’s rainy season (May to August), the study observed a relative reduction in the training model’s R-square value. This phenomenon is attributed to temporal discrepancies between Himawari-8 and the MAIAC products during this period. The predictive models of PM2.5 concentrations with the identification of pertinent variables through Pearson’s correlation analysis and recursive feature elimination, driven by the robust XGBoost model. Subsequently, the downscaled AOD, wind speed, temperature, and pressure were identified as predictors for the estimation of hourly PM2.5 concentration. This comprehensive approach enabled the projection of PM2.5 levels across Thailand, encompassing over 600,000 grids at 1 × 1 km2 resolution. The developed models, thus, offer a valuable tool for robust and high-resolution PM2.5 concentration estimation, presenting significant implications for air quality monitoring and management in Thailand.
Exploring KRAS-mutant pancreatic ductal adenocarcinoma: a model validation study
Authors: Yang Fan , He Yanjie , Ge Nan , Guo Jintao , Yang Fei , Sun Siyu
Published: January 2024

Introduction

Pancreatic ductal adenocarcinoma (PDAC) has the highest mortality rate among all solid tumors. Tumorigenesis is promoted by the oncogene KRAS, and KRAS mutations are prevalent in patients with PDAC. Therefore, a comprehensive understanding of the interactions between KRAS mutations and PDAC may expediate the development of therapeutic strategies for reversing the progression of malignant tumors. Our study aims at establishing and validating a prediction model of KRAS mutations in patients with PDAC based on survival analysis and mRNA expression.

Methods

A total of 184 and 412 patients with PDAC from The Cancer Genome Atlas (TCGA) database and the International Cancer Genome Consortium (ICGC), respectively, were included in the study.

Results

After tumor mutation profile and copy number variation (CNV) analyses, we established and validated a prediction model of KRAS mutations, based on survival analysis and mRNA expression, that contained seven genes: CSTF2, FAF2, KIF20B, AKR1A1, APOM, KRT6C, and CD70. We confirmed that the model has a good predictive ability for the prognosis of overall survival (OS) in patients with KRAS-mutated PDAC. Then, we analyzed differential biological pathways, especially the ferroptosis pathway, through principal component analysis, pathway enrichment analysis, Gene Ontology (GO) enrichment analysis, and gene set enrichment analysis (GSEA), with which patients were classified into low- or high-risk groups. Pathway enrichment results revealed enrichment in the cytokine-cytokine receptor interaction, metabolism of xenobiotics by cytochrome P450, and viral protein interaction with cytokine and cytokine receptor pathways. Most of the enriched pathways are metabolic pathways predominantly enriched by downregulated genes, suggesting numerous downregulated metabolic pathways in the high-risk group. Subsequent tumor immune infiltration analysis indicated that neutrophil infiltration, resting CD4 memory T cells, and resting natural killer (NK) cells correlated with the risk score. After verifying that the seven gene expression levels in different KRAS-mutated pancreatic cancer cell lines were similar to that in the model, we screened potential drugs related to the risk score.

Discussion

This study established, analyzed, and validated a model for predicting the prognosis of PDAC based on risk stratification according to KRAS mutations, and identified differential pathways and highly effective drugs.
Persistence of KIRneg NK cells after haploidentical hematopoietic stem cell transplantation protects from human cytomegalovirus infection/reactivation
Authors: Di Vito Clara , Coianiz Nicolò , Calvi Michela , Terzoli Sara , Zaghi Elisa , Puccio Simone , Frigo Alessandro , Mariotti Jacopo , De Philippis Chiara , Mannina Daniele , Sarina Barbara , Mineri Rossana , Le-Trilling Vu Thuy Khanh , Trilling Mirko , Castagna Luca , Bramanti Stefania , Santoro Armando , Mavilio Domenico
Published: January 2024

Haploidentical hematopoietic stem cell transplantation (h-HSCT) is a therapeutic option to cure patients affected by hematologic malignancies. The kinetics and the quality of immune-reconstitution (IR) impact the clinical outcome of h-HSCT and limit the onset of life-threatening Human Cytomegalovirus (HCMV) infection/reactivation. Natural Killer (NK) cells are the first lymphocytes that recover after h-HSCT and they can provide rapid innate immune responses against opportunistic pathogens. By performing a longitudinal single-cell analysis of multiparametric flow-cytometry data, we show here that the persistence at high frequencies of CD158b1b2jneg/NKG2Apos/NKG2Cneg/NKp30pos/NKp46pos (KIRneg) NK cells is associated with HCMV infection/reactivation control. These KIRneg NK cells are “unlicensed”, and are not terminal-differentiated lymphocytes appearing early during IR and mainly belonging to CD56bright/CD16neg and CD56bright/CD16pos subsets. KIRneg NK cells are enriched in oxidative and glucose metabolism pathways, produce interferon-γ, and are endowed with potent antiviral activity against HCMV ex vivo. Decreased frequencies of KIRneg NK cells early during IR are associated with clinically relevant HCMV replication. Taken together, our findings indicate that the prolonged persistence of KIRneg NK cells after h-HSCT could serve as a biomarker to better predict HCMV infection/reactivation. This phenomenon also paves the way to optimize anti-viral immune responses by enriching post-transplant donor lymphocyte infusions with KIRneg NK cells.
TCRβ clones in muscle tissue share structural features in patients with idiopathic inflammatory myopathy and are associated with disease activity
Authors: Anang Dornatien C. , Walter Hannah A. W. , Lim Johan , Niewold Ilse T. G. , van der Weele Linda , Aronica Eleonora , Eftimov Filip , Raaphorst Joost , van Schaik Barbera D. C. , van Kampen Antoine H. C. , van der Kooi Anneke J. , de Vries Niek
Published: January 2024

Objectives

To characterize the T cell receptor (TCRβ) repertoire in peripheral blood and muscle tissues of treatment naïve patients with newly diagnosed idiopathic inflammatory myopathies (IIMs).

Methods

High throughput RNA sequencing of the TCRβ chain was performed in peripheral blood and muscle tissue in twenty newly-diagnosed treatment-naïve IIM patients (9 DM, 5 NM/OM, 5 IMNM and 1 ASyS) and healthy controls. Results thereof were correlated with markers of disease activity.

Results

Muscle tissue of IIM patients shows more expansion of TCRβ clones and decreased diversity when compared to peripheral blood of IIM as well as healthy controls (both p=0.0001). Several expanded TCRβ clones in muscle are tissue restricted and cannot be retrieved in peripheral blood. These clones have significantly longer CDR3 regions when compared to clones (also) found in circulation (p=0.0002), while their CDR3 region is more hydrophobic (p<0.01). Network analysis shows that clonal TCRβ signatures are shared between patients. Increased clonal expansion in muscle tissue is significantly correlated with increased CK levels (p=0.03), while it tends to correlate with decreased muscle strength (p=0.08).

Conclusion

Network analysis of clones in muscle of IIM patients shows shared clusters of sequences across patients. Muscle-restricted CDR3 TCRβ clones show specific structural features in their T cell receptor. Our results indicate that clonal TCRβ expansion in muscle tissue might be associated with disease activity. Collectively, these findings support a role for specific clonal T cell responses in muscle tissue in the pathogenesis of the IIM subtypes studied.
Antigen-presenting B cells promote TCF-1+ PD1- stem-like CD8+ T-cell proliferation in glioblastoma
Authors: Hou David , Wan Hanxiao , Katz Joshua L. , Wang Si , Castro Brandyn A. , Vazquez-Cervantes Gustavo I. , Arrieta Victor A. , Dhiantravan Silpol , Najem Hinda , Rashidi Aida , Chia Tzu-yi , Arjmandi Tarlan , Collado Jimena , Billingham Leah , Lopez-Rosas Aurora , Han Yu , Sonabend Adam M. , Heimberger Amy B. , Zhang Peng , Miska Jason , Lee-Chang Catalina
Published: January 2024

Understanding the spatial relationship and functional interaction of immune cells in glioblastoma (GBM) is critical for developing new therapeutics that overcome the highly immunosuppressive tumor microenvironment. Our study showed that B and T cells form clusters within the GBM microenvironment within a 15-μm radius, suggesting that B and T cells could form immune synapses within the GBM. However, GBM-infiltrating B cells suppress the activation of CD8+ T cells. To overcome this immunosuppression, we leveraged B-cell functions by activating them with CD40 agonism, IFNγ, and BAFF to generate a potent antigen-presenting B cells named BVax. BVax had improved antigen cross-presentation potential compared to naïve B cells and were primed to use the IL15-IL15Ra mechanism to enhance T cell activation. Compared to naïve B cells, BVax could improve CD8 T cell activation and proliferation. Compared to dendritic cells (DCs), which are the current gold standard professional antigen-presenting cell, BVax promoted highly proliferative T cells in-vitro that had a stem-like memory T cell phenotype characterized by CD62L+CD44- expression, high TCF-1 expression, and low PD-1 and granzyme B expression. Adoptive transfer of BVax-activated CD8+ T cells into tumor-bearing brains led to T cell reactivation with higher TCF-1 expression and elevated granzyme B production compared to DC-activated CD8+ T cells. Adoptive transfer of BVax into an irradiated immunocompetent tumor-bearing host promoted more CD8+ T cell proliferation than adoptive transfer of DCs. Moreover, highly proliferative CD8+ T cells in the BVax group had less PD-1 expression than those highly proliferative CD8+ T cells in the DC group. The findings of this study suggest that BVax and DC could generate distinctive CD8+ T cells, which potentially serve multiple purposes in cellular vaccine development.
Comparative transcriptomic insights into the domestication of Pleurotus abieticola for coniferous cultivation
Authors: Sun Lei , Sossah Frederick Leo , Li Yu , Sun Huiying
Published: January 2024

Introduction:Pleurotus abieticola, a promising edible fungus in the Pleurotaceae family, especially its ability to utilize coniferous substrate, holds significant potential for commercial cultivation. However, few reports on the adaptation of P. abieticola to coniferous substrate from the perspective of omics.

Methods: This study explores the biological characteristics, domestication process, and nutritional composition of P. abieticola, along with its adaptability to coniferous substrates using transcriptomics. We assessed biological characteristics, optimizing mycelial growth on agar medium with varied carbon and nitrogen sources, temperature, and pH. Additionally, the optimization process extended to fruiting bodies, where impact on the differentiation were evaluated under varying light conditions. Fruiting body nutrient composition was analyzed per the Chinese National Food Safety Standard. Transcriptome sequencing focused on P. abieticola mycelial colonized coniferous and broadleaved substrates.

Results and Discussion: The optimal conditions for mycelial growth were identified: dextrin (carbon source), diammonium hydrogen phosphate (nitrogen source), 25°C (temperature), and pH 7.0. White light promoted fruiting body growth and differentiation. Larch substrate exhibited superior yield (190 g) and biological efficiency (38.0%) compared to oak (131 g, 26.2%) and spruce (166 g, 33.2%). P. abieticola showcased high dietary fiber, protein, and total sugar content, low fat, and sufficient microelements. Transcriptome analysis revealed significant key genes involved in lignocellulose degradation, stress-resistant metabolism, and endocytosis metabolism, underscoring their pivotal for coniferous adaptation. This study offers valuable insights for the commercial development and strain breeding of P. abieticola, efficiently leveraging conifer resources. The findings underscore its potential as a valuable source for food, medicinal products, and biotechnological applications.
Intruders or protectors – the multifaceted role of B cells in CNS disorders
Authors: Aspden James W. , Murphy Matthew A. , Kashlan Rommi D. , Xiong Yueyue , Poznansky Mark C. , Sîrbulescu Ruxandra F.
Published: January 2024

B lymphocytes are immune cells studied predominantly in the context of peripheral humoral immune responses against pathogens. Evidence has been accumulating in recent years on the diversity of immunomodulatory functions that B cells undertake, with particular relevance for pathologies of the central nervous system (CNS). This review summarizes current knowledge on B cell populations, localization, infiltration mechanisms, and function in the CNS and associated tissues. Acute and chronic neurodegenerative pathologies are examined in order to explore the complex, and sometimes conflicting, effects that B cells can have in each context, with implications for disease progression and treatment outcomes. Additional factors such as aging modulate the proportions and function of B cell subpopulations over time and are also discussed in the context of neuroinflammatory response and disease susceptibility. A better understanding of the multifactorial role of B cell populations in the CNS may ultimately lead to innovative therapeutic strategies for a variety of neurological conditions.
Characterizing a new clinical phenotype: the co-existence of cerebral venous outflow and connective tissue disorders
Authors: Midtlien Jackson P. , Curry Brian P. , Chang Emily , Kiritsis Nicholas R. , Aldridge Jennifer B. , Fargen Kyle M.
Published: January 2024

Background

There is increasing recognition of connective tissue disorders and their influence on disease in the general population. A conserved clinical phenotype involving connective tissue disorders and idiopathic intracranial hypertension (IIH) and associated cerebral venous outflow disorders (CVD) has not been previously described.

Methods

A single-institution retrospective review of a prospectively maintained database of patients with connective tissue disorders and CVD was performed.

Results

A total of 86 patients were identified. The majority of these patients carried a diagnosis of Ehlers-Danlos syndrome (55%) and most were non-obese (mean body mass index 29.7 kg/m2), Caucasian (90%) females (87%). Most prevalent presenting symptoms included pressure headache (98%), dizziness (90%), tinnitus (92%), and cognitive dysfunction (69%). Aside from CVD and IIH, the most common associated conditions were postural orthostatic tachycardia syndrome (POTS; 55.8%), cerebrospinal fluid (CSF) leaks (51.2%), dysautonomia (45.3%), cranio-cervical instability (37.2%), mast cell activation syndrome (25.6%), and tethered cord syndrome (23.3%). Allergies to medications (87.2%) and surgical tape (19.8%) were also frequent. Despite significantly lower opening pressures on lumbar puncture, headache severity and quality of life scores were reported with the same severity of classic IIH patients, suggesting an underlying hypersensitivity to intracranial pressures and cerebral venous congestion.

Conclusion

There is a rare but conserved clinical phenotype that has not been described previously that presents with severe IIH symptoms in predominantly young, non-obese Caucasian women with a high associated incidence of dysautonomia, POTS, craniocervical instability, and CSF leaks, among others.
The application of radionuclide therapy for breast cancer
Authors: Musket Anna , Davern Sandra , Elam Brianna M. , Musich Philip R. , Moorman Jonathan P. , Jiang Yong
Published: January 2024

Radionuclide-mediated diagnosis and therapy have emerged as effective and low-risk approaches to treating breast cancer. Compared to traditional anatomic imaging techniques, diagnostic radionuclide-based molecular imaging systems exhibit much greater sensitivity and ability to precisely illustrate the biodistribution and metabolic processes from a functional perspective in breast cancer; this transitions diagnosis from an invasive visualization to a noninvasive visualization, potentially ensuring earlier diagnosis and on-time treatment. Radionuclide therapy is a newly developed modality for the treatment of breast cancer in which radionuclides are delivered to tumors and/or tumor-associated targets either directly or using delivery vehicles. Radionuclide therapy has been proven to be eminently effective and to exhibit low toxicity when eliminating both primary tumors and metastases and even undetected tumors. In addition, the specific interaction between the surface modules of the delivery vehicles and the targets on the surface of tumor cells enables radionuclide targeting therapy, and this represents an exceptional potential for this treatment in breast cancer. This article reviews the development of radionuclide molecular imaging techniques that are currently employed for early breast cancer diagnosis and both the progress and challenges of radionuclide therapy employed in breast cancer treatment.
Association between body weight perception and intuitive eating among undergraduate students in China: the mediating role of body image
Authors: Zhu Yuanyuan , Gao Jiage , Gao Qinyi , Chen Dandan , Zeng Zhi
Published: January 2024

Background

The association between body weight perception and intuitive eating among undergraduate students in China remains insufficiently understood. In the present study, we were aimed to examine the correlation between body weight perception, body image, and intuitive eating and determine whether the link between body weight perception and intuitive eating was influenced by body image.

Methods

A total of 1,050 undergraduate students completed the survey. Participants provided self-reported demographic details and completed two structured scales. The Body Esteem Scale for Adolescents and Adults (BESAA) and the Intuitive Eating Scale-2 (IES-2) were employed to assess body image and intuitive eating. Analysis of the mediation model was conducted using version 4.1 of the PROCESS Macro. Results with a value of p less than 0.05 were deemed statistically significant.

Results

The average age of the participants was 20.08 years (SD = 1.64). Among the students, 837 (79.7%) were female, and 212 (20.3%) were male. Body image (r = −0.429, p < 0.001) and intuitive eating (r = −0.313, p < 0.001) exhibited significant negative associations with body weight perception. Furthermore, body image showed a significant positive correlation with intuitive eating (r = 0.318, p < 0.001). Significant mediating effects of body image were identified concerning intuitive eating and body weight perception in the right weight (95% bootstrap CI = 0.007, 0.040) and overweight groups (95% bootstrap CI = −0.048, −0.009). The indirect effects of body image constituted 12.19% and 15.33% of the total effects of intuitive eating in these two groups.

Conclusion

Although the indirect effects were not substantial, these outcomes shed light on the partial understanding of how body weight perception impacted intuitive eating via body image. Importantly, our findings emphasized the significance of body image and body weight perception, offering a novel insight for prospective interventions targeting undergraduate students.
Radical gastrectomy is safe for treatment of gastric cancer patients on immunosuppressive drugs after organ transplantation
Authors: Lee Jin Ah , Kim So Jung , Seo Ho Seok , Lee Han Hong , Kim Sung Geun , Jun Kyong Hwa , Song Kyo Young , Jung Yoon Ju
Published: January 2024

Background

De novo malignancies are major causes of death after organ transplantation because the recipients subsequently receive immunosuppressant drugs. When gastric cancer develops, the clinical course of the tumor may be particularly aggressive. However, there are few reliable studies of gastric cancer treatment after organ transplantation. This study examined the clinicopathological characteristics of gastric cancer patients after organ transplantation and evaluated treatment outcomes after gastrectomy.

Methods

Clinical data were collected from 54 patients who were diagnosed with gastric cancer after organ transplantation. Of these, 30 who underwent surgery for gastric cancer while on immunosuppressant medications were compared with a control group of 625 gastric cancer patients. To compensate for clinical differences between the two groups, 1:1 propensity-score matching was performed.

Results

Among the 30 gastric cancer patients on immunosuppressants, kidney transplantation was the most common procedure (19/30, 63.3%) followed by bone marrow (6) and liver transplantation (4); among all 54 patients, 45 were on one or two immunosuppressants. Up-migration to an advanced pathological stage was more frequent in the transplant group. In multivariate analysis, transplantation was a significant risk factor for up-migration from the T, M, and final stages after surgery. When the 30 patients on immunosuppressants who underwent gastric cancer surgery were compared with the matched controls, the total incidence (30.0 vs 40.0%, P = 0.417) and the number of severe postoperative complications (16.7 vs 13.4%, P = 0.417) did not differ significantly between groups after propensity score matching. In terms of overall survival, the transplant group showed significantly worse prognosis in stages I, II, and IV (P < 0.001, P = 0.039 and 0.007, respectively).

Conclusion

Radical gastrectomy can be a safe oncological procedure for gastric cancer patients on immunosuppressants after transplantation. Considering their immunosuppressed condition and the possibility of underestimation of the stage of gastric cancer, early detection with endoscopic screening is needed to allow curative treatment.
Pathologic and immunohistochemical prognostic markers in residual triple-negative breast cancer after neoadjuvant chemotherapy
Authors: Ilie Silvia Mihaela , Briot Nathalie , Constatin Guillaume , Ilie Alis , Beltjens Francoise , Ladoire Sylvain , Desmoulins Isabelle , Hennequin Audrey , Bertaut Aurelie , Coutant Charles , Causeret Sylvain , Ghozali Niama , Coudert Bruno , Arnould Laurent
Published: January 2024

Background

The persistence of residual tumour after neoadjuvant chemotherapy (NAC) in localised triple-negative breast cancer (TNBC) is known to have a negative prognostic value. However, different degrees of expression of some immunohistochemical markers may correlate with different prognoses.

Methods

The expression of biomarkers with a known prognostic value, i.e., cytokeratin 5/6 (CK5/6), androgen receptor (AR), epidermal growth factor receptor (EGFR) proliferation-related nuclear antigen Ki-67, human epidermal growth factor receptor 2 (HER2), protein 53 (p53), forkhead box protein 3 (FOXP3), and cluster differentiation 8 (CD8), was analysed by immunohistochemistry in 111 samples after NAC in non-metastatic TNBC patients addressed to Georges-François Leclerc Cancer Centre Dijon, France. Clinical and pathological variables were retrospectively collected. Cox regression was used to identify immunohistochemical (IHC) and clinicopathological predictors of event-free survival (EFS) (relapse or death).

Results

Median age was 50.4 years (range 25.6–88.3), 55.9% (n = 62) were non-menopausal, 70 (63.1%) had stage IIA–IIB disease. NAC was mostly sequential anthracycline-taxanes (72.1%), and surgical intervention was principally conservative (51.3%). We found 65.7% ypT1, 47.2% lymph node involvement (ypN+), and 29.4% lymphovascular invasion (LVI). Most residual tumours were EGFR >110 (H-score) (60.5%, n = 66), AR ≥4% (53.2%, n = 58), p53-positive mutated (52.7%, n = 58), CD8 ≥26 (58.1%, n = 61), FOXP3 ≥7 (51.4%, n = 54), more than half in the stroma, and 52.3% (n = 58) HER2 score 0. After a median follow-up of 80.8 months, 48.6% had relapsed. Median EFS was 62.3 months (95% CI, 37.2–not reached (NR)). Factors independently associated with poor EFS were AR-low (p = 0.002), ypN+ (p < 0.001), and LVI (p = 0.001). Factors associated with lower overall survival (OS) were EGFR-low (p = 0.041), Ki-67 high (p = 0.024), and ypN+ (p < 0.001).

Conclusion

Post-NAC residual disease in TNBC showed biomarkers specific to a basal-like subtype and markers of lymphocyte infiltration mostly present in the stroma. Prognostic markers for EFS were AR, LVI, and ypN and warrant further validation in a prognostic model.
Pediatric intensive care unit admissions network—rationale, framework and method of operation of a nationwide collaborative pediatric intensive care research network in Germany
Authors: Bruns Nora , Dohna-Schwake Christian , Olivieri Martin , Urschitz Michael S. , Blomenkamp Susanne , Frosch Clara , Lieftüchter Victoria , Tomidis Chatzimanouil Markos K. , Hoffmann Florian , Brenner Sebastian
Published: January 2024

The Pediatric Intensive Care Unit Admissions (PIA) network aims to establish a nationwide database in Germany to gather epidemiological, clinical, and outcome data on pediatric critical illness. The heterogeneity of pediatric patients in intensive care units (PICU) poses challenges in obtaining sufficient case numbers for reliable research. Multicentered approaches, such as patient registries, have proven effective in collecting large-scale data. However, Germany lacks a systematic registration system for pediatric intensive care admissions, hindering epidemiological and outcome assessments. The PIA network intends to address these gaps and provide a framework for clinical and epidemiological research in pediatric intensive care. The network will interconnect PICUs across Germany and collect structured data on diagnoses, treatment, clinical course, and short-term outcomes. It aims to identify areas for improvement in care, enable disease surveillance, and potentially serve as a quality control tool. The PIA network builds upon the existing infrastructure of the German Pediatric Surveillance Unit ESPED and utilizes digitalized data collection techniques. Participating units will complete surveys on their organizational structure and equipment. The study population includes patients aged ≥28 days admitted to participating PICUs, with a more detailed survey for cases meeting specific criteria. Data will be collected by local PIA investigators, anonymized, and entered into a central database. The data protection protocol complies with regulations and ensures patient privacy. Quarterly data checks and customized quality reports will be conducted to monitor data completeness and plausibility. The network will evaluate its performance, data collection feasibility, and data quality. Eligible investigators can submit proposals for data analyses, which will be reviewed and analyzed by trained statisticians or epidemiologists. The PIA network aims to improve pediatric intensive care medicine in Germany by providing a comprehensive understanding of critical illness, benchmarking treatment quality, and enabling disease surveillance.
Effects of ketamine on rat social behavior as analyzed by DeepLabCut and SimBA deep learning algorithms
Authors: Popik Piotr , Cyrano Ewelina , Piotrowska Diana , Holuj Malgorzata , Golebiowska Joanna , Malikowska-Racia Natalia , Potasiewicz Agnieszka , Nikiforuk Agnieszka
Published: January 2024

Traditional methods of rat social behavior assessment are extremely time-consuming and susceptible to the subjective biases. In contrast, novel digital techniques allow for rapid and objective measurements. This study sought to assess the feasibility of implementing a digital workflow to compare the effects of (R,S)-ketamine and a veterinary ketamine preparation Vetoquinol (both at 20 mg/kg) on the social behaviors of rat pairs. Historical and novel videos were used to train the DeepLabCut neural network. The numerical data generated by DeepLabCut from 14 video samples, representing various body parts in time and space were subjected to the Simple Behavioral Analysis (SimBA) toolkit, to build classifiers for 12 distinct social and non-social behaviors. To validate the workflow, previously annotated by the trained observer historical videos were analyzed with SimBA classifiers, and regression analysis of the total time of social interactions yielded R2 = 0.75, slope 1.04; p < 0.001 (N = 101). Remarkable similarities between human and computer annotations allowed for using the digital workflow to analyze 24 novel videos of rats treated with vehicle and ketamine preparations. Digital workflow revealed similarities in the reduction of social behavior by both compounds, and no substantial differences between them. However, the digital workflow also demonstrated ketamine-induced increases in self-grooming, increased transitions from social contacts to self-grooming, and no effects on adjacent lying time. This study confirms and extends the utility of deep learning in analyzing rat social behavior and highlights its efficiency and objectivity. It provides a faster and objective alternative to human workflow.
Therapeutic drug monitoring of corticosteroids/β2-agonists in the hair of patients with asthma: an open-label feasibility study
Authors: Salvator Hélène , Lamy Elodie , Roquencourt Camille , Bardin Emmanuelle , Devillier Philippe , Grassin-Delyle Stanislas
Published: January 2024

Background: Although adherence to inhaled medication is critically important for treatment efficiency, around half of patients taking these drugs are non-adherent or make critical errors when using their delivery device. Segmental hair analysis might be a valuable tool for therapeutic monitoring because hair concentrations reflect exposure from month to month. The objective of the present proof-of-concept study was to establish the feasibility of segmental hair analysis of inhaled budesonide and formoterol in asthma patients.

Methods: We conducted a prospective, open-label, interventional study of adult patients being treated with budesonide/formoterol for controlled, moderate-to-severe asthma (CorticHair, NCT03691961). Asthma control, lung function, and medication adherence were recorded. Hair samples were taken 4 months after enrolment and cut into four 1 cm segments.

Results: Samples were available from 21 patients (20 women; median age: 53; median budesonide dose: 600 μg/d). Budesonide and formoterol were detected in samples from 18 to 13 patients, respectively. The median hair concentrations were 6.25 pg/mg for budesonide and 0.9 pg/mg for formoterol. The intrapatient coefficient of variation between hair segments was 21% for budesonide and 40% for formoterol. Pearson’s coefficients for the correlations between the hair concentration and the self-reported drug dose and the prescribed drug dose were respectively 0.42 (p = 0.08) and 0.29 (p = 0.25) for budesonide and 0.24 (p = 0.44) and 0.17 (p = 0.57) for formoterol.

Conclusion: Segmental hair analysis of inhaled medications was feasible, with low intrapatient variability. This innovative, non-invasive means of assessing monthly drug exposure might help physicians to personalize drug regimens for patients with difficult-to-treat asthma.
Gender and social class inequalities in higher education: intersectional reflections on a workshop experience
Authors: Fernandez Daniela , Orazzo Emily , Fry Emma , McMain Alice , Ryan Michelle K. , Wong Chuk Yan , Begeny Christopher T.
Published: January 2024

Research about the experiences of underrepresented groups in higher education (HE) demonstrates the persistence of challenges, despite policies and institutional strategies to promote inclusion. Diversity and inclusion policies have been part of the HE agenda for several decades, yet most policies and interventions focus on (a) a given, isolated identity experience (e.g., based solely on gender, social class, or ethnicity) rather than more intersectional approaches to identity; and (b) top-down interventions that do not include participants insights in their design. In this paper, we report a case study of a workshop with students at an elite university that drew on an intersectional approach to social identities (IASI), specifically, looking at gender and social class. We explore three key themes: (a) the importance of group processes, (b) the use of visual techniques, and (c) the institutional tensions and the (de)politicisation of social psychology research. Reflecting on this case study we argue that approaches to identity and inclusion in HE can benefit from intersectionality beyond the use of multi and overlapping identity and social group categories. We argue that research in this space is not neutral and needs to acknowledge researchers’ position about (a) inclusion and diversity, (b) perceptions of participants in research, and (c) the motivation and aims of institutions where the research is conducted. Finally, we discuss the theoretical and practical implications of integrating an intersectional approach within social identity research in HE when focusing on underrepresented groups.
Explore the effects of forest travel activities on university students’ stress affection
Authors: Chang Wei-Yin , Wang Xin , Guo De-Sheng , Nguyen Lam-Huu-Phuoc , Tran Ngoc-Huy , Yang Shuai-Jie , Lin Hui-Zhong , Wu Hsiu-Chen , Huang Chin-Fei
Published: January 2024

This study aims to explore the effects of forest travel activities on university students’ stress affection. Forty volunteer university students participated in this study. All participants were asked to complete physiological (Heart Rate Variability) and psychological (Brief Profile of Mood State and State–Trait Anxiety Inventory) tests before and after the travel activities. The results reported that students’ heart rates were significantly lower after the forest travel activities than before. All domains of negative mood and anxiety decreased from the pre-test to the post-test. This study found that university students could feel less stressed if they went on forest travel activities.
Using an extended model of the reasoned action approach to explore individual behavioral intentions regarding litter and plastic pollution prevention in a developing country
Authors: Oduro-Appiah Kwaku , Afful Abraham , Osei-Tutu Henrietta
Published: January 2024

Implementing litter and plastic pollution prevention strategies is essential for cities of developing countries, mainly due to the prevailing high incidence of littering and the urgent need to realize the adverse per capita environmental impact target of the sustainable development goals. In this article, we report the use of the prominent reasoned action approach—in its original state and an extended model with moral norms—for exploring the critical socio-cognitive determinants of individuals’ litter prevention intentions in Ghana. By analyzing the valid answers of 447 participants to a structured questionnaire on litter prevention, we found attitudes (β = 0.35, SE = 0.014, p < 0.001) and moral norms (β = 0.57, SE = 0.099, p < 0.001) as the most influencing determinants to individual intentions in the original and the extended models, respectively. The analysis suggests that individuals will stop littering their environments if environmentally friendly interventions are implemented to elicit self-responsibility and moral obligation. Campaigns that demonstrate the effects of littering on drain blockage, flooding, and disease outbreaks may improve individual litter prevention attitudes. Installing waste receptacles in public spaces and communicating persuasive messages may facilitate personal antilittering intentions. Apart from contributing to the implementation of a litter management strategy to reduce the flood risk and enhance the resilience of the Greater Accra region of Ghana, this research helps to close the literature gaps in litter prevention behavior in developing countries, as well as support the implementation of the sustainable development goals and the global plastic action partnership.