bluuebunny/crossref_metadata_2025 huggingface dataset:
Quantitative Ultrastructural Morphometry and Gene Expression of mTOR-Related Mitochondriogenesis within Glioblastoma Cells
Authors: Ferese Rosangela , Lenzi Paola , Fulceri Federica , Biagioni Francesca , Fabrizi Cinzia , Gambardella Stefano , Familiari Pietro , Frati Alessandro , Limanaqi Fiona , Fornai Francesco
Published: June 2020

In glioblastoma (GBM) cells, an impairment of mitochondrial activity along with autophagy suppression occurs. Autophagy suppression in GBM promotes stemness, invasion, and poor prognosis. The autophagy deficit seems to be due, at least in part, to an abnormal up-regulation of the mammalian target of rapamycin (mTOR), which may be counteracted by pharmacological mTORC1 inhibition. Since autophagy activation is tightly bound to increased mitochondriogenesis, a defect in the synthesis of novel mitochondria is expected to occur in GBM cells. In an effort to measure a baseline deficit in mitochondria and promote mitochondriogenesis, the present study used two different GBM cell lines, both featuring mTOR hyperactivity. mTORC1 inhibition increases the expression of genes and proteins related to autophagy, mitophagy, and mitochondriogenesis. Autophagy activation was counted by RT-PCR of autophagy genes, LC3- immune-fluorescent puncta and immune-gold, as well as specific mitophagy-dependent BNIP3 stoichiometric increase in situ, within mitochondria. The activation of autophagy-related molecules and organelles after rapamycin exposure occurs concomitantly with progression of autophagosomes towards lysosomes. Remarkably, mitochondrial biogenesis and plasticity (increased mitochondrial number, integrity, and density as well as decreased mitochondrial area) was long- lasting for weeks following rapamycin withdrawal.
The Potential Contribution of Dysfunctional RNA-Binding Proteins to the Pathogenesis of Neurodegeneration in Multiple Sclerosis and Relevant Models
Authors: Libner Cole D. , Salapa Hannah E. , Levin Michael C.
Published: June 2020

Neurodegeneration in multiple sclerosis (MS) is believed to underlie disease progression and permanent disability. Many mechanisms of neurodegeneration in MS have been proposed, such as mitochondrial dysfunction, oxidative stress, neuroinflammation, and RNA-binding protein dysfunction. The purpose of this review is to highlight mechanisms of neurodegeneration in MS and its models, with a focus on RNA-binding protein dysfunction. Studying RNA-binding protein dysfunction addresses a gap in our understanding of the pathogenesis of MS, which will allow for novel therapies to be generated to attenuate neurodegeneration before irreversible central nervous system damage occurs.
Biosensors for D-Amino Acids: Detection Methods and Applications
Authors: Rosini Elena , D’Antona Paola , Pollegioni Loredano
Published: June 2020

D-enantiomers of amino acids (D-AAs) are only present in low amounts in nature, frequently at trace levels, and for this reason, their biological function was undervalued for a long time. In the past 25 years, the improvements in analytical methods, such as gas chromatography, HPLC, and capillary electrophoresis, allowed to detect D-AAs in foodstuffs and biological samples and to attribute them specific biological functions in mammals. These methods are time-consuming, expensive, and not suitable for online application; however, life science investigations and industrial applications require rapid and selective determination of D-AAs, as only biosensors can offer. In the present review, we provide a status update concerning biosensors for detecting and quantifying D-AAs and their applications for safety and quality of foods, human health, and neurological research. The review reports the main challenges in the field, such as selectivity, in order to distinguish the different D-AAs present in a solution, the simultaneous assay of both L- and D-AAs, the production of implantable devices, and surface-scanning biosensors. These innovative tools will push future research aimed at investigating the neurological role of D-AAs, a vibrant field that is growing at an accelerating pace.
Treatment Efficiency in Gaucher Patients Can Reliably Be Monitored by Quantification of Lyso-Gb1 Concentrations in Dried Blood Spots
Authors: Cozma Claudia , Cullufi Paskal , Kramp Guido , Hovakimyan Marina , Velmishi Virtut , Gjikopulli Agim , Tomori Sonila , Fischer Steffen , Oppermann Sebastian , Grittner Ulrike , Bauer Peter , Beetz Christian , Rolfs Arndt
Published: June 2020

Gaucher disease (GD) is a lysosomal storage disorder that responds well to enzyme replacement therapy (ERT). Certain laboratory parameters, including blood concentration of glucosylsphingosine (Lyso-Gb1), the lyso-derivate of the common glycolipid glucocerebroside, correlate with clinical improvement and are therefore considered candidate-monitoring biomarkers. Whether they can indicate a reduction or loss of treatment efficiency, however, has not been systematically addressed for obvious reasons. We established and validated measurement of Lyso-Gb1 from dried blood spots (DBSs) by mass spectrometry. We then characterized the assay’s longitudinal performance in 19 stably ERT-treated GD patients by dense monitoring over a 3-year period. The observed level of fluctuation was accounted for in the subsequent development of a unifying data normalization concept. The resulting approach was eventually applied to data from Lyso-Gb1 measurements after an involuntary treatment break for all 19 patients. It enabled separation of the “under treatment” versus “not under treatment” conditions with high sensitivity and specificity. We conclude that Lyso-Gb1 determination from DBSs indicates treatment issues already at an early stage before clinical consequences arise. In addition to its previously shown diagnostic utility, Lyso-Gb1 thereby qualifies as a monitoring biomarker in GD patients.
Brachytherapy in a Single Dose of 10Gy as an “in situ” Vaccination
Authors: Jarosz-Biej Magdalena , Smolarczyk Ryszard , Cichoń Tomasz , Drzyzga Alina , Czapla Justyna , Urbaś Zbigniew , Pilny Ewelina , Matuszczak Sybilla , Wojcieszek Piotr
Published: June 2020

Radiotherapy (RT) is one of the major methods of cancer treatment. RT destroys cancer cells, but also affects the tumor microenvironment (TME). The delicate balance between immunomodulation processes in TME is dependent, among other things, on a specific radiation dose. Despite many studies, the optimal dose has not been clearly determined. Here, we demonstrate that brachytherapy (contact radiotherapy) inhibits melanoma tumor growth in a dose-dependent manner. Doses of 10Gy and 15Gy cause the most effective tumor growth inhibition compared to the control group. Brachytherapy, at a single dose of ≥ 5Gy, resulted in reduced tumor blood vessel density. Only a dose of 10Gy had the greatest impact on changes in the levels of tumor-infiltrating immune cells. It most effectively reduced the accumulation of protumorogenic M2 tumor-associated macrophages and increased the infiltration of cytotoxic CD8+ T lymphocytes. To summarize, more knowledge about the effects of irradiation doses in anticancer therapy is needed. It may help in the optimization of RT treatment. Our results indicate that a single dose of 10Gy leads to the development of a robust immune response. It seems that it is able to convert a tumor microenvironment into an “in situ” vaccine and lead to a significant inhibition of tumor growth.
Influence of Intermittent Cold Stimulations on CREB and Its Targeting Genes in Muscle: Investigations into Molecular Mechanisms of Local Cryotherapy
Authors: Sugasawa Takehito , Tome Yoshiya , Takeuchi Yoshinori , Yoshida Yasuko , Yahagi Naoya , Sharma Rahul , Aita Yuichi , Ueda Haruna , Maruyama Reina , Takeuchi Kaoru , Morita Shohei , Kawamai Yasushi , Takekoshi Kazuhiro
Published: June 2020

Local cryotherapy is widely used as a treatment for sports-related skeletal muscle injuries. The molecular mechanisms are unknown. To clarify these mechanisms, we applied one to three 15-min cold stimulations at 4 °C to various cell lines (in vitro), the tibialis anterior (TA) muscle (ex vivo), and mouse limbs (in vivo). In the in vitro assay, cyclic AMP (cAMP) response element binding protein 1 (CREB1) was markedly phosphorylated (p-CREB1), and the CREB-binding protein (CBP) was recruited to p-CREB-1 in response to two or three cold stimulations. In a reporter assay with the cAMP-responsive element, the signals significantly increased after two to three cold stimulations at 4 °C. In the ex vivo study, CREB-targeting genes were significantly upregulated following two or three cold stimulations. The in vivo experiment disclosed that cold stimulation of a mouse limb for 9 days significantly increased mitochondrial DNA copy number and upregulated genes involved in mitochondrial biogenesis. The results suggest that local cryotherapy increases CREB transcription and upregulates CREB-targeting genes, in a manner dependent on cold stimulation frequency and duration. This information will inform further investigations into local cryotherapy as a treatment for sports-related skeletal muscle trauma.
Self-Organized Liver Microtissue on a Bio-Functional Surface: The Role of Human Adipose-Derived Stromal Cells in Hepatic Function
Authors: Hong Seokheon , Oh Seung Ja , Choi Dongho , Hwang Yongsung , Kim Sang-Heon
Published: June 2020

The maintenance of hepatocyte function is a critical research topic in liver tissue engineering. Although an increasing number of strategies have been developed, liver tissue engineering using hepatocytes as a therapeutic alternative remains challenging owing to its poor efficacy. In this study, we developed a multicellular hepatic microtissue to enhance the function of induced hepatic precursor cells. Mouse induced hepatic precursor cells (miHeps) were self-organized in 3D with human adipose-derived stem cells (hASCs) on a bio-functional matrix. We found that hepatic phenotypes, such as levels of albumin, asialoglycoprotein receptor-1, and cytochrome P450, were enhanced in miHeps-hASC microtissue comprising miHeps and hASCs relative to two-dimensional-cultured miHeps-hASCs. Additionally, the secretome of 3D-cultured hASCs increased the hepatic function of mature miHeps. Furthermore, hepatic gene expression was reduced in mature miHeps treated with conditioned media of hypoxia-inducible factor 1α (HIF1α)-depleted hASCs relative to that with conditioned media of control hASCs. Our results suggested that the hepatic function of 3D-co-cultured miHeps could be enhanced by HIF1α-dependent factors secreted from stromal cells. This study provides an insight into the factors regulating hepatic function and shows that self-organized hepatic microtissue could act as liver spheroids for liver regenerative medicine and liver toxicity tests.
Exploration of the Effects of Different Blue LED Light Intensities on Flavonoid and Lipid Metabolism in Tea Plants via Transcriptomics and Metabolomics
Authors: Wang Pengjie , Chen Sirong , Gu Mengya , Chen Xiaomin , Chen Xuejin , Yang Jiangfan , Zhao Feng , Ye Naixing
Published: June 2020

Blue light extensively regulates multiple physiological processes and secondary metabolism of plants. Although blue light quantity (fluence rate) is important for plant life, few studies have focused on the effects of different blue light intensity on plant secondary metabolism regulation, including tea plants. Here, we performed transcriptomic and metabolomic analyses of young tea shoots (one bud and two leaves) under three levels of supplemental blue light, including low-intensity blue light (LBL, 50 μmol m–2 s–1), medium-intensity blue light (MBL, 100 μmol m–2 s–1), and high-intensity blue light (HBL, 200 μmol m–2 s–1). The total number of differentially expressed genes (DEGs) in LBL, MBL and HBL was 1, 7 and 1097, respectively, indicating that high-intensity blue light comprehensively affects the transcription of tea plants. These DEGs were primarily annotated to the pathways of photosynthesis, lipid metabolism and flavonoid synthesis. In addition, the most abundant transcription factor (TF) families in DEGs were bHLH and MYB, which have been shown to be widely involved in the regulation of plant flavonoids. The significantly changed metabolites that we detected contained 15 lipids and 6 flavonoid components. Further weighted gene co-expression network analysis (WGCNA) indicated that CsMYB (TEA001045) may be a hub gene for the regulation of lipid and flavonoid metabolism by blue light. Our results may help to establish a foundation for future research investigating the regulation of woody plants by blue light.
HMGB1-Mediated Neuroinflammatory Responses in Brain Injuries: Potential Mechanisms and Therapeutic Opportunities
Authors: Paudel Yam Nath , Angelopoulou Efthalia , Piperi Christina , Othman Iekhsan , Shaikh Mohd. Farooq
Published: June 2020

Brain injuries are devastating conditions, representing a global cause of mortality and morbidity, with no effective treatment to date. Increased evidence supports the role of neuroinflammation in driving several forms of brain injuries. High mobility group box 1 (HMGB1) protein is a pro-inflammatory-like cytokine with an initiator role in neuroinflammation that has been implicated in Traumatic brain injury (TBI) as well as in early brain injury (EBI) after subarachnoid hemorrhage (SAH). Herein, we discuss the implication of HMGB1-induced neuroinflammatory responses in these brain injuries, mediated through binding to the receptor for advanced glycation end products (RAGE), toll-like receptor4 (TLR4) and other inflammatory mediators. Moreover, we provide evidence on the biomarker potential of HMGB1 and the significance of its nucleocytoplasmic translocation during brain injuries along with the promising neuroprotective effects observed upon HMGB1 inhibition/neutralization in TBI and EBI induced by SAH. Overall, this review addresses the current advances on neuroinflammation driven by HMGB1 in brain injuries indicating a future treatment opportunity that may overcome current therapeutic gaps.
Molecular Evidence that Lysiphlebia japonica Regulates the Development and Physiological Metabolism of Aphis gossypii
Authors: Gao Xueke , Xue Hui , Luo Junyu , Ji Jichao , Zhang Lijuan , Niu Lin , Zhu Xiangzhen , Wang Li , Zhang Shuai , Cui Jinjie
Published: June 2020

Lysiphlebia japonica Ashmead (Hymenoptera, Braconidae) is an endophagous parasitoid and Aphis gossypii Glover (Hemiptera, Aphididae) is a major pest in cotton. The relationship between insect host-parasitoids and their hosts involves complex physiological, biochemical and genetic interactions. This study examines changes in the development and physiological metabolism of A. gossypii regulated by L. japonica. Our results demonstrated that both the body length and width increased compared to non-parasitized aphids. We detected significantly increases in the developmental period as well as severe reproductive castration following parasitization by L. japonica. We then used proteomics to characterize these biological changes, and when combined with transcriptomes, this analysis demonstrated that the differential expression of mRNA (up or downregulation) captured a maximum of 48.7% of the variations of protein expression. We assigned these proteins to functional categories that included immunity, energy metabolism and transport, lipid metabolism, and reproduction. We then verified the contents of glycogen and 6-phosphate glucose, which demonstrated that these important energy sources were significantly altered following parasitization. These results uncover the effects on A. gossypii following parasitization by L. japonica, additional insight into the mechanisms behind insect-insect parasitism, and a better understanding of host-parasite interactions.
Genetic Variants in the FGB and FGG Genes Mapping in the Beta and Gamma Nodules of the Fibrinogen Molecule in Congenital Quantitative Fibrinogen Disorders Associated with a Thrombotic Phenotype
Authors: Simurda Tomas , Brunclikova Monika , Asselta Rosanna , Caccia Sonia , Zolkova Jana , Kolkova Zuzana , Loderer Dusan , Skornova Ingrid , Hudecek Jan , Lasabova Zora , Stasko Jan , Kubisz Peter
Published: June 2020

Fibrinogen is a hexameric plasmatic glycoprotein composed of pairs of three chains (Aα, Bβ, and γ), which play an essential role in hemostasis. Conversion of fibrinogen to insoluble polymer fibrin gives structural stability, strength, and adhesive surfaces for growing blood clots. Equally important, the exposure of its non-substrate thrombin-binding sites after fibrin clot formation promotes antithrombotic properties. Fibrinogen and fibrin have a major role in multiple biological processes in addition to hemostasis and thrombosis, i.e., fibrinolysis (during which the fibrin clot is broken down), matrix physiology (by interacting with factor XIII, plasminogen, vitronectin, and fibronectin), wound healing, inflammation, infection, cell interaction, angiogenesis, tumour growth, and metastasis. Congenital fibrinogen deficiencies are rare bleeding disorders, characterized by extensive genetic heterogeneity in all the three genes: FGA, FGB, and FGG (enconding the Aα, Bβ, and γ chain, respectively). Depending on the type and site of mutations, congenital defects of fibrinogen can result in variable clinical manifestations, which range from asymptomatic conditions to the life-threatening bleeds or even thromboembolic events. In this manuscript, we will briefly review the main pathogenic mechanisms and risk factors leading to thrombosis, and we will specifically focus on molecular mechanisms associated with mutations in the C-terminal end of the beta and gamma chains, which are often responsible for cases of congenital afibrinogenemia and hypofibrinogenemia associated with thrombotic manifestations.
Bispicolyamine-Based Supramolecular Polymeric Gels Induced by Distinct Different Driving Forces with and Without Zn2+
Authors: Park Jaehyeon , Kim Ka Young , Kang Seok Gyu , Lee Shim Sung , Lee Ji Ha , Jung Jong Hwa
Published: June 2020

Metal-coordination polymeric gels are interesting areas as organic/inorganic hybrid supramolecular materials. The bispicolylamine (BPA) based gelator (1) showed excellent gelation with typical fibrillar morphology in acetonitrile. Upon complexing 1 with Zn2+, complexes ([1 + Zn + ACN]2+ and [1 + zinc trifluoromethanesulfonate (ZnOTf)]+) with four coordination numbers were formed, which determine the gel structure significantly. A gel-sol transition was induced, driven by the ratio of the two metal complexes produced. Through nuclear magnetic resonance analysis, the driving forces in the gel formation (i.e., hydrogen-bonding and π–π stacking) were observed in detail. In the absence and the presence of Zn2+, the intermolecular hydrogen-bonds and π–π stacking were the primary driving forces in the gel formation, respectively. In addition, the supramolecular gels exhibited a monolayer lamellar structure irrespective of Zn2+. Conclusively, the gels’ elasticity and viscosity reduced in the presence of Zn2+.
Effects of Apigenin on RBL-2H3, RAW264.7, and HaCaT Cells: Anti-Allergic, Anti-Inflammatory, and Skin-Protective Activities
Authors: Park Che-Hwon , Min Seon-Young , Yu Hye-Won , Kim Kyungmin , Kim Suyeong , Lee Hye-Ja , Kim Ji-Hye , Park Young-Jin
Published: June 2020

Apigenin (4′,5,7-trihydroxyflavone, flavonoid) is a phenolic compound that is known to reduce the risk of chronic disease owing to its low toxicity. The first study on apigenin analyzed its effect on histamine release in the 1950s. Since then, anti-mutation and antitumor properties of apigenin have been widely reported. In the present study, we evaluated the apigenin-mediated amelioration of skin disease and investigated its applicability as a functional ingredient, especially in cosmetics. The effect of apigenin on RAW264.7 (murine macrophage), RBL-2H3 (rat basophilic leukemia), and HaCaT (human immortalized keratinocyte) cells were analyzed. Apigenin (100 μM) significantly inhibited nitric oxide (NO) production, cytokine expression (interleukin (IL)-1β, IL6, cyclooxygenase (COX)-2, and inducible nitric oxide synthase [iNOS]), and phosphorylation of mitogen-activated protein kinase (MAPK) signal molecules, including extracellular signal-regulated kinase (ERK) and c-Jun N-terminal protein kinase (JNK) in RAW264.7 cells. Apigenin (30 μM) also inhibited the phosphorylation of signaling molecules (Lyn, Syk, phospholipase Cγ1, ERK, and JNK) and the expression of high-affinity IgE receptor FcεRIα and cytokines (tumor necrosis factor (TNF)-α, IL-4, IL-5, IL-6, IL-13, and COX-2) that are known to induce inflammation and allergic responses in RBL-2H3 cells. Further, apigenin (20 μM) significantly induced the expression of filaggrin, loricrin, aquaporin-3, hyaluronic acid, hyaluronic acid synthase (HAS)-1, HAS-2, and HAS-3 in HaCaT cells that are the main components of the physical barrier of the skin. Moreover, it promoted the expression of human β-defensin (HBD)-1, HBD-2, HBD-3, and cathelicidin (LL-37) in HaCaT cells. These antimicrobial peptides are known to play an important role in the skin as chemical barriers. Apigenin significantly suppressed the inflammatory and allergic responses of RAW264.7 and RBL cells, respectively, and would, therefore, serve as a potential prophylactic and therapeutic agent for immune-related diseases. Apigenin could also be used to improve the functions of the physical and chemical skin barriers and to alleviate psoriasis, acne, and atopic dermatitis.
Seismic Assessment of Six Typologies of Existing RC Bridges
Authors: Crespi Pietro , Zucca Marco , Longarini Nicola , Giordano Nicola
Published: June 2020

Over the last few decades, the attention on the safety of existing reinforced concrete (RC) structures has significantly increased. RC bridges, in particular, are highly relevant for strategic importance. In the Italian context, several of these bridges were built around 1960, when engineering practice commonly ignored or underestimated the presence of seismic actions. Therefore, it is fundamental to quantify as accurately as possible their seismic safety level with state-of-the-art analysis techniques. In this paper, an efficient procedure based on the multi-modal pushover analysis approach is proposed for the risk evaluation of several bridges of the Italian highway network. This procedure, tailored for portfolio level assessment, takes into account the non-linear behavior and the complex dynamic response this type of structure with limited computational effort. Three fundamental aspects are defined for the structural modelling of bridges, i.e., materials’ constitutive law, finite element type and nonlinear hinge models. Flexural and shear nonlinearities of piers are included to account for ductile and brittle damage potential. The standardized procedure guarantees consistent comparisons among different bridges of the same network in the form of risk indexes.
Miniaturised Rod-Shaped Polymer Structures with Wire or Fibre Reinforcement—Manufacturing and Testing
Authors: Kucher Michael , Dannemann Martin , Heide Ansgar , Winkler Anja , Modler Niels
Published: June 2020

Rod-shaped polymer-based composite structures are applied to a wide range of applications in the process engineering, automotive, aviation, aerospace and marine industries. Therefore, the adequate knowledge of manufacturing methods is essential, covering the fabrication of small amounts of specimens as well as the low-cost manufacturing of high quantities of solid rods using continuous manufacturing processes. To assess the different manufacturing methods and compare the resulting quality of the semi-finished products, the cross-sectional and bending properties of rod-shaped structures obtained from a thermoplastic micro-pultrusion process, conventional fibre reinforced epoxy resin-based solid rods and fibre reinforced thermoplastic polymers manufactured by means of an implemented shrink tube consolidation process, were statistically analysed. Using the statistical method one-way analysis of variance (ANOVA), the differences between groups were calculated. The statistical results show that the flexural moduli of carbon fibre reinforced polymers were statistically significantly higher than the modulus of all other investigated specimens (probability value ). The discontinuous shrink tube consolidation process resulted in specimens with a smooth outer contour and a high level of roundness. However, this process was recommended for the manufacturing of small amounts of specimens. In contrast, the pultrusion process allowed the manufacturing of high amounts of semi-finished products; however, it requires a more extensive process controlling and manufacturing equipment.
Analyzing Age-Related Macular Degeneration Progression in Patients with Geographic Atrophy Using Joint Autoencoders for Unsupervised Change Detection
Authors: Dupont Guillaume , Kalinicheva Ekaterina , Sublime Jérémie , Rossant Florence , Pâques Michel
Published: June 2020

Age-Related Macular Degeneration (ARMD) is a progressive eye disease that slowly causes patients to go blind. For several years now, it has been an important research field to try to understand how the disease progresses and find effective medical treatments. Researchers have been mostly interested in studying the evolution of the lesions using different techniques ranging from manual annotation to mathematical models of the disease. However, artificial intelligence for ARMD image analysis has become one of the main research focuses to study the progression of the disease, as accurate manual annotation of its evolution has proved difficult using traditional methods even for experienced practicians. In this paper, we propose a deep learning architecture that can detect changes in the eye fundus images and assess the progression of the disease. Our method is based on joint autoencoders and is fully unsupervised. Our algorithm has been applied to pairs of images from different eye fundus images time series of 24 ARMD patients. Our method has been shown to be quite effective when compared with other methods from the literature, including non-neural network based algorithms that still are the current standard to follow the disease progression and change detection methods from other fields.
Restoration of Uterine Cavity Measurements after Surgical Correction
Authors: Detti Laura , Christiansen Mary Emily , Levi D’Ancona Roberto , Gordon Jennifer C. , Van de Velde Nicole , Peregrin-Alvarez Irene
Published: June 2020

Objective: We sought to define the uterine and uterine cavity dimensions of subseptate uteri before and after hysteroscopic surgical incision, and compare them to those obtained in normal uteri with 3-D ultrasound. Methods: Two cohorts of consecutive women with normal-appearing uterine cavity and women diagnosed with uterine subseptations, before and after undergoing hysteroscopic incision. 3-D ultrasound was used to measure the uterine cavity width, length, and area on a frozen coronal view of the uterus. Results: A total of 215 women were included: 89 in the normal, and 126 in the subseptate uterus, groups. Uterine length and height were similar in the pre-operative, post-operative subseptate uteri, and in the normal uteri, while the uterine width was significantly greater in the pre-operative (5.1 + 0.8 cm) than post-operative (4.7 + 0.8 cm) and normal uterus (4.6 + 0.7 cm; p < 0.001) groups. The pre-operative uterine cavity length (3.3 + 0.5 cm), width (3.2 + 0.7 cm), and area (4.4 + 1.2 cm2), were significantly greater than the post-operative ones (length 2.9 + 0.4 cm; width 2.6 + 0.6 cm; area 3.7 + 0.8 cm; overall p < 0.001), and became similar to the dimensions of the normal uterus. Of the patients who subsequently conceived, 2.6% miscarried in the corrected subseptation group and 28.8% miscarried in the normal uterus group. Conclusions: We defined the ultrasound dimensions of the uterine cavity in subseptate uteri and their change after surgical correction. Uterine cavity length, width, and area show very little variability in adult normal uteri, while they are increased in uteri with a subseptation greater than 5.9 mm in length, and regain normal measurements after surgical correction.
Estimating the Annual Exceedance Probability of Water Levels and Wave Heights from High Resolution Coupled Wave-Circulation Models in Long Island Sound
Authors: Liu Chang , Jia Yan , Onat Yaprak , Cifuentes-Lorenzen Alejandro , Ilia Amin , McCardell Grant , Fake Todd , O’Donnell James
Published: June 2020

Accurately estimating the probability of storm surge occurrences is necessary for flood risk assessments. This research models Long Island Sound using a coupled coastal circulation and wave model (FVCOM-SWAVE) to hindcast the 44 highest storms between 1950–2018 and fitted Poisson-GPD distributions to modelled water levels and wave heights. Floodwater elevations and significant wave heights for 10% (1/10), 3% (1/30), 2% (1/50), and 1% (1/100) annual exceedance probabilities are provided for all Connecticut coastal towns. The results show that both water levels and their corresponding return intervals are higher along the western coast of Connecticut than the eastern coast, whereas significant wave heights increase eastward. Comparing our model results with those from the North Atlantic Coast Comprehensive Study (NACCS) shows that the mean NACCS results are higher for water levels and lower for significant wave heights for longer return periods. Likewise, the Federal Emergency Management Agency (FEMA) results in large errors compared to our results in both eastern and western coastal Connecticut regions. In addition to evaluating historical risks, we also added a sea-level height offset of 0.5 m for 2050 estimates in order to examine the effect of rising sea-levels on the analysis. We find that sea-level rise reduces the return period of a 10-year storm to two years. We advise periodically updating this work as improved sea-level rise projections become available.
Distribution of Bisphenol A in Sediment and Suspended Matter and Its Possible Impact on Marine Life in Kaštela Bay, Adriatic Sea, Croatia
Authors: Anđelić Ivana , Roje-Busatto Romana , Ujević Ivana , Vuletić Nenad , Matijević Slavica
Published: June 2020

Over the last decade, bisphenol A (BPA) has become a chemical of concern in the marine environment. There is little data on BPA levels in the eastern Adriatic Sea, Croatian waters. This study provides concentrations of BPA in marine sediments and suspended matter sampled from the Kaštela Bay (the central part of the Adriatic Sea) for two years. The results obtained show that BPA in sediment samples ranged from 1.05 to 46.31 µg kg−1, while they were higher in the suspended matter, ranging from 1.84 to 81.39 µg kg−1. To demonstrate a possible correlation between BPA concentrations and other parameters in sediment and suspended matter, the granulometric composition and organic matter content were determined. There was no correlation between BPA and investigated sediment characteristics. To assess the possible effects of BPA on marine biota, its possible estrogenic effect was also investigated by calculating estradiol equivalent concentration (EEQ, µg kg−1), the maximum value of which, in this study, was 0.0181 µg kg−1 in the sediment samples and 0.0317 µg kg−1 in suspended matter samples. Based on the presented results, it is unlikely that BPA could cause significant endocrine disruption to marine life in the study area.
Marine Algae Polysaccharides as Basis for Wound Dressings, Drug Delivery, and Tissue Engineering: A Review
Authors: Kuznetsova Tatyana A. , Andryukov Boris G. , Besednova Natalia N. , Zaporozhets Tatyana S. , Kalinin Andrey V.
Published: June 2020

The present review considers the physicochemical and biological properties of polysaccharides (PS) from brown, red, and green algae (alginates, fucoidans, carrageenans, and ulvans) used in the latest technologies of regenerative medicine (tissue engineering, modulation of the drug delivery system, and the design of wound dressing materials). Information on various types of modern biodegradable and biocompatible PS-based wound dressings (membranes, foams, hydrogels, nanofibers, and sponges) is provided; the results of experimental and clinical trials of some dressing materials in the treatment of wounds of various origins are analyzed. Special attention is paid to the ability of PS to form hydrogels, as hydrogel dressings meet the basic requirements set out for a perfect wound dressing. The current trends in the development of new-generation PS-based materials for designing drug delivery systems and various tissue-engineering scaffolds, which makes it possible to create human-specific tissues and develop target-oriented and personalized regenerative medicine products, are also discussed.
CPAnet Registry—An International Chronic Pulmonary Aspergillosis Registry
Authors: Laursen Christian B. , Davidsen Jesper Rømhild , Van Acker Lander , Salzer Helmut J.F. , Seidel Danila , Cornely Oliver A. , Hoenigl Martin , Alastruey-Izquierdo Ana , Hennequin Christophe , Godet Cendrine , Barac Aleksandra , Flick Holger , Munteanu Oxana , Van Braeckel Eva
Published: June 2020

Chronic pulmonary aspergillosis (CPA) is a chronic fungal infection of the lung associated with high morbidity and mortality. The CPA Research network (CPAnet) registry established in 2018 is an international multicenter collaboration aiming to improve CPA knowledge and patient care. This study’s aim was to describe the data collection process and content of CPAnet registry with preliminary clinical data. In the CPAnet registry, clinical data are collected through a web-based questionnaire. Data include CPA phenotype, comorbidities, treatment, outcome, and follow-up from several international centers. An exemplary descriptive analysis was performed on 74 patients, who were registered online before April 2020. CPA patients were predominantly (72%) male, 39% had chronic obstructive pulmonary disease, and 68% had a history of smoking. Chronic cavitary pulmonary aspergillosis was the most common CPA subtype (62%). In 32 patients (52%), voriconazole was the preferred first-line therapy. The multicenter multinational CPAnet registry is a valuable approach to gather comprehensive data on a large study population and reflects real-world clinical practice rather than focusing on specific patient populations in more specialized centers. Additional CPA reference centers are being encouraged to join this promising clinical research collaboration.
Nursing Personnel in the Era of Personalized Healthcare in Clinical Practice
Authors: Spanakis Marios , Patelarou Athina E. , Patelarou Evridiki
Published: June 2020

Personalized, stratified, or precision medicine (PM) introduces a new era in healthcare that tries to identify and predict optimum treatment outcomes for a patient or a cohort. It also introduces new scientific terminologies regarding therapeutic approaches and the need of their adoption from healthcare providers. Till today, evidence-based practice (EBP) was focusing on population averages and their variances among cohorts for clinical values that are essential for optimizing healthcare outcome. It can be stated that EBP and PM are complementary approaches for a modern healthcare system. Healthcare providers through EBP often see the forest (population averages) but miss the trees (individual patients), whereas utilization of PM may not see the forest for the trees. Nursing personnel (NP) play an important role in modern healthcare since they are consulting, educating, and providing care to patients whose needs often needs to be individualized (personalized nursing care, PNC). Based on the clinical issues earlier addressed from clinical pharmacology, EBP, and now encompassed in PM, this review tries to describe the challenges that NP have to face in order to meet the requisites of the new era in healthcare. It presents the demands that should be met for upgrading the provided education and expertise of NP toward an updated role in a modern healthcare system.
Technology Acceptance in e-Governance: A Case of a Finance Organization
Authors: Mohammad Ebrahimzadeh Sepasgozar Fatemeh , Ramzani Usef , Ebrahimzadeh Sabbar , Sargolzae Sharifeh , Sepasgozar Samad
Published: June 2020

Presently, one of the most critical challenges for e-government and e-banking is the accurate and correct realization of factors that have a significant impact on customer behavior. Without appropriate knowledge of these factors, it would be impossible to predict the level of welcoming toward new services, acquire a competitive advantage, and coordinate marketing programs with the needs of customers. On the other hand, in today’s competitive world, banks are obliged to implement new services to retain current customers and attract new ones. This research has been conducted with the goal of identifying influential factors that have an impact on the development of user intentions. The theoretical research model has been designed based on the technology acceptance model (TAM), as well as technology adoption theory, technology dissemination theory, and planned behavior theory. This study adopted an empirical approach to investigate key acceptance factors in a case organization. The statistical population of this research consists of customers and employees in different branches of a financial institution called Mehr bank in Iran. The data was collected by means of questionnaires that were completed by 200 customers and employees who work at Mehr bank or have business relationships with it. Data analysis in descriptive and inferential statistics domains had been done in SPSS and AMOS software, respectively. This paper presents first-hand data analysis of a case study on technology adoption in banking systems in Iran. In addition, structural equations have been used for inferential analysis. The findings of this study confirm the direct impact of “perceived usefulness” and “perceived ease of use” towards user attitudes. In addition, results show that “attitude” and “perceived usefulness” have a direct impact on the development of usage intention in customers. However, the results do not confirm the role of subjective norms on the development of user intent. This study is limited to a selected organization, and the proposed model should be examined by applying it in different contexts.
Untangle the Complex Stakeholder Relationships in Rural Settlement Consolidation in China: A Social Network Approach
Authors: Yang Fan , Chi Guangqing , Wang Ge , Tang Shirui , Li Yunting , Ju Cong
Published: June 2020

Rural settlement consolidation (RSC) has a critical role in facilitating the transformation of human settlement and land use transition in the rural revitalization process. RSC involves a diversity of stakeholder groups with complex and intertwined concerns. It is therefore crucial to identify the key stakeholders and their main concerns to effectively align rural planning and policymaking. However, this line of research remains underdeveloped. This study provides a novel and holistic network perspective for unpacking the complex relationships among different stakeholders. The results indicate: (1) the network of stakeholder concerns is relatively sparse, with 68 concern nodes and 159 concern ties; (2) The village committee, centralized residents, and contractors occupy the core position within the concerns network, while the local government has the majority of strongly connected nodes; (3) The lists of prominent concern nodes and ties are identified by different network indices, including the degree difference, the out-status centrality, closeness centrality, node betweenness centrality, and link betweenness centrality; (4) The main interaction type among stakeholder groups can be classified into five categories: financing, psychological attachment, stakeholder participation, project management, and the improvement in living conditions and infrastructure. This study reveals the relatively weak status of residents, the pivotal role of the village committee, as well as the indispensable part of the contractor and township government, with the aim to provide targeted guidance and decision-making supports for strengthening interactions and cooperation among different stakeholder groups. The findings shed new light on performing the multi-tasks of RSC and facilitating the sustainable management of rural areas.
Simple, Accurate and User-Friendly Differential Constitutive Model for the Rheology of Entangled Polymer Melts and Solutions from Nonequilibrium Thermodynamics
Authors: S. Stephanou Pavlos , Ch. Tsimouri Ioanna , G. Mavrantzas Vlasis
Published: June 2020

In a recent reformulation of the Marrucci-Ianniruberto constitutive equation for the rheology of entangled polymer melts in the context of nonequilibrium thermodynamics, rather large values of the convective constraint release parameter βccr had to be used in order for the model not to violate the second law of thermodynamics. In this work, we present an appropriate modification of the model, which avoids the splitting of the evolution equation for the conformation tensor into an orientation and a stretching part. Then, thermodynamic admissibility simply dictates that βccr ≥ 0, thus allowing for more realistic values of βccr to be chosen. Moreover, and in view of recent experimental evidence for a transient stress undershoot (following the overshoot) at high shear rates, whose origin may be traced back to molecular tumbling, we have incorporated additional terms into the model accounting, at least in an approximate way, for non-affine deformation through a slip parameter ξ. Use of the new model to describe available experimental data for the transient and steady-state shear and elongational rheology of entangled polystyrene melts and concentrated solutions shows close agreement. Overall, the modified model proposed here combines simplicity with accuracy, which renders it an excellent choice for managing complex viscoelastic fluid flows in large-scale numerical calculations.
Performance of Short Fiber Interlayered Reinforcement Thermoplastic Resin in Additive Manufacturing
Authors: Fan Congze , Shan Zhongde , Zou Guisheng , Zhan Li , Yan Dongdong
Published: June 2020

To further improve the mechanical properties of thermoplastic resin in additive manufacturing (AM), this paper presents a novel method to directly and quantitatively place the short fibers (SFs) between two printing process of resin layers. The printed composite parts with SFs between the layers was reinforced. The effects of single-layer fiber content, multi-layer fiber content and the length of fibers on the mechanical properties of printed specimens were studied. The distribution of fibers and quality of interlayer bonding were assessed using mechanical property testing and microstructure examination. The results showed that the tensile strength of the single-layered specimen with 0.5 wt% interlayered SFs increased by 18.82%. However, when the content of SFs continued to increase, the mechanical properties declined because of the increasing interlayered gap and the poor bonding quality. In addition, when the interlayered SFs length was 0.5–1 mm, the best reinforcement was obtained. To improve the interfacial bonding quality between the fiber and the resin, post-treatment and laser-assisted preheating printing was used. This method is effective for the enhancement of the interfacial bonding to obtain better mechanical properties. The research proves that adding SFs by placement can reduce the wear and breakage of the fibers compared to the conventional forming process. Therefore, the precise control of the length and content of SFs was realized in the specimen. In summary, SFs placement combined with post-treatment and laser-assisted preheating is a new method in AM to improve the performance of thermoplastic resin.
Texture and Differential Stress Development in W/Ni-Co Composite after Rotary Swaging
Authors: Strunz Pavel , Kocich Radim , Canelo-Yubero David , Macháčková Adéla , Beran Přemysl , Krátká Ludmila
Published: June 2020

Knowledge of texture and residual stresses in tungsten heavy pseudoalloys is substantial for the microstructure optimization. These characteristics were determined in cold and warm rotary swaged W/NiCo composite with help of neutron diffraction. The results were discussed in view of the observed microstructure and mechanical properties. The investigated bars consisted of tungsten agglomerates (bcc lattice) surrounded by NiCo-based matrix (fcc lattice). No preferential crystallographic orientation was found in the as-sintered bar. A strong texture was formed in both the tungsten agglomerates (<101> fiber texture parallel to the swaging axis) and in the NiCo-based matrix (<111> fiber texture) after rotary swaging. Although usually of double-fiber texture, the <001> fiber of the fcc structures was nearly missing in the matrix. Further, the cold-swaged bar exhibited substantially stronger texture for both phases which corresponds to the higher measured ultimate tensile strength. The residual stress differences were employed for characterization of the stress state of the bars. The largest residual stress difference (≈400 MPa) was found at the center of the bar deformed at room temperature. The hoop stresses were non-symmetrical with respect to the swaging axis, which was likely caused by the elliptical cross section of the as-sintered bar.
An Insight into Ionic Conductivity of Polyaniline Thin Films
Authors: Chulkin Pavel , Łapkowski Mieczysław
Published: June 2020

The work addresses an issue of the conductivity phenomenon in conductive polymer thin films. Polyaniline was chosen as a broadly used and thoroughly investigated conductive polymer in order to test and show capabilities of the developed original approach based on impedance spectra analysis. A number of films of different thickness were deposited onto a Pt electrode surface and consequently investigated in aqueous solution containing perchloric acid as an electrolyte. The processes that occur in polyaniline film were studied by cyclic voltammetry, electrochemical quartz crystal microgravimetry (EQCM) and electrochemical impedance spectroscopy (EIS). The role of incorporated ions as charge carriers was investigated with respect to the control of the conductivity properties of the film. Along with detailed polyaniline behavior study, the work makes up a fundamental scientific impact on theoretical electrochemistry and electroanalytical techniques.