bluuebunny/crossref_metadata_2025 huggingface dataset:
Microscopic Mechanical Properties and Physicochemical Changes of Cement Paste Exposed to Elevated Temperatures and Subsequent Rehydration
Authors: Xu Lei , Hu Xiaochuan , Tang Ruifeng , Zhang Xin , Xia Yan , Ran Bo , Liu Jinlong , Zhuang Shiyu , Tian Weichen
Published: February 2025

The effect of elevated temperatures and subsequent rehydration on the microscopic mechanical properties and physicochemical changes of cement pastes was investigated. Cement pastes with different grades (CEM I 42.5, CEM I 52.5) and different water-to-cement ratios (0.3, 0.4) were exposed to target temperatures of 300 °C, 600 °C, and 900 °C, followed by rehydration. Several characterization techniques, including the Vickers microhardness test, X-ray diffraction, thermogravimetry, and 1H Nuclear Magnetic Resonance spectroscopy, were employed to assess changes in the microscopic mechanical and physicochemical properties of the cement pastes resulting from the heating and rehydration treatments. The results indicate that the cement pastes with higher grades and a higher water-to-cement ratio exhibit better resistance to high temperatures. The heating process alters the water distribution and structure of C-S-H gel, leading to the collapse of its interlayer structure and an increase in gel porosity. Elevated temperatures (300 °C and 600 °C), followed by rehydration, enhance the Vickers microhardness of the cement pastes. However, excessively high temperatures (900 °C) weaken the micro-mechanical properties and may cause damage. Cement pastes heated to 600 °C show a more significant recovery in micro-mechanical properties compared to those heated at 300 °C, which is attributed to the rehydration of a new amorphous nesosilicate phase formed at 600 °C.
Determination of Constraint-Independent Crack Tip Opening Angle for Stable Crack Growth in High-Strength Ductile Steels
Authors: Zhu Xian-Kui
Published: February 2025

The crack tip opening angle (CTOA) is one of fracture toughness parameters that has been used for decades in describing large stable crack growth in thin-walled aerospace structures under the low-constraint conditions. Recently, the pipeline industry has developed a growing interest in using the CTOA parameter to serve as the minimum required fracture toughness to arrest dynamic crack propagation in modern gas transmission pipelines made of high-strength ductile steel. To meet this industrial need, the CTOA test standard ASTM E3039 was therefore developed for measuring the constant critical CTOA. ASTM E3039 recommends a drop weight tearing test (DWTT) specimen with a shallow crack for standard CTOA testing, but its CTOA may depend on the low constraint condition of the DWTT specimen at the crack tip. Verifying the constraint independence of the DWTT-measured CTOA thus becomes indispensable for applying CTOA toughness to the running fracture control in the pipeline design. For this purpose, the present paper evaluates critical CTOA values in a set of fracture toughness tests on single-edge notched bend (SENB) specimens with shallow and deep cracks, based on four CTOA estimation models. Among these, the Ln(P)-LLD linear fit model is similar to that recommended by ASTM E3039 for CTOA calculation. Fracture test data for X80 pipeline steel and HY80 structural steel were considered in the CTOA evaluation. The results showed that the four CTOA models were able to determine a constraint independent CTOA value for stable crack growth in the SENB specimens. As a result, a single, reliable, constant CTOA value could be determined regardless of the specimen geometry or the crack-tip constraint conditions. Therefore, the CTOA measured using ASTM E3039 is constraint-independent and transferable to use in cases of actual cracks propagating in gas transmission pipelines.
BERT Mutation: Deep Transformer Model for Masked Uniform Mutation in Genetic Programming
Authors: Shem-Tov Eliad , Sipper Moshe , Elyasaf Achiya
Published: February 2025

We introduce BERT mutation, a novel, domain-independent mutation operator for Genetic Programming (GP) that leverages advanced Natural Language Processing (NLP) techniques to improve convergence, particularly using the Masked Language Modeling approach. By combining the capabilities of deep reinforcement learning and the BERT transformer architecture, BERT mutation intelligently suggests node replacements within GP trees to enhance their fitness. Unlike traditional stochastic mutation methods, BERT mutation adapts dynamically by using historical fitness data to optimize mutation decisions, resulting in more effective evolutionary improvements. Through comprehensive evaluations across three benchmark domains, we demonstrate that BERT mutation significantly outperforms conventional and state-of-the-art mutation operators in terms of convergence speed and solution quality. This work represents a pivotal step toward integrating state-of-the-art deep learning into evolutionary algorithms, pushing the boundaries of adaptive optimization in GP.
Analysis of Fatty Acid Profile, α-Tocopherol, Squalene and Cholesterol Content in Edible Parts and By-Products of South Pacific Wild Fishes
Authors: López-Puebla Sussi , Arias-Santé María Fernanda , Romero Jaime , Costa de Camargo Adriano , Rincón-Cervera Miguel Ángel
Published: February 2025

Fish are generally rich sources of n-3 polyunsaturated fatty acids such as EPA and DHA, and although the edible part (fillet) has been analyzed in many species, less is known about the composition of fish by-products. The analysis of these materials allows them to be evaluated as raw sources of EPA and DHA, thus contributing to sustainable practices to produce healthy oils for human consumption. This work provides information on the fatty acid profiles, lipid quality indices and α-tocopherol, squalene and cholesterol contents in fillets, heads, bones and viscera of three fish species (anchovy, chub mackerel and Chilean jack mackerel). Samples were lyophilized and lipids were extracted using either the Folch or Hara and Radin methods. FA profiles were obtained by gas chromatography coupled with flame ionization detection, and tocopherol, squalene and cholesterol analyses were performed by high performance liquid chromatography with UV-visible detection. The highest levels of EPA were found in anchovy fillet (18.9–20.6%) and bone (14.7%), while DHA was more abundant in anchovy fillet (16.9–22.0%) and Chilean jack mackerel fillet (15.4–16.6%) and bone (13.1–13.8%). α-Tocopherol, squalene and cholesterol contents ranged from 0.18 to 1.35 mg/100 g, 0.07 to 0.80 mg/100 g and 30.46 to 246.17 mg/100 g, respectively, in the different tissues analyzed from the three fish species.
Implications of Heart Failure Treatment on Atrial Fibrillation Onset: A Retrospective Study
Authors: Suhov Loredana , Apostol Adrian , Dăniluc Larissa , Haj Ali Lina , Sandu Oana Elena , Bogdan Carina , Andor Minodora
Published: February 2025

Background and Objectives: Atrial fibrillation (AF) is one of the most common supraventricular arrhythmias in the adult population worldwide and it is frequently associated with heart failure (HF). The coexistence of these conditions increases morbidity, mortality and reduces quality of life in these patients. Therefore, it is important to delay the onset of AF in HF patients in order to avoid complications. The study aims to assess whether HF treatment influences AF onset. Materials and Methods: This retrospective observational study included 260 patients, 144 patients with heart failure treated with sodium–glucose cotransporter 2 inhibitors (SGLT2i) and 116 patients with heart failure without SGLT2i treatment (control group) hospitalized at least twice in the Cardiology Department of the “Pius Brinzeu” Emergency County Hospital between 2022 and 2024. Results: Treatment with SGLT2i was associated with a lower prevalence of atrial fibrillation in patients with heart failure. Conclusions: The study highlights the cardiovascular benefits of SGLT2 inhibitors and suggests a potential effect on the onset of AF in heart failure patients.
Correction: Liang et al. Effect of Al2O3 on Inclusion Removal in H13 Steels Using High-Basicity LF (Ladle Furnace) Refining Slags. Metals 2023, 13, 1592
Authors: Liang Ting , Qin Zhuo , Wang Linzhu
Published: February 2025

In the original publication [...]
Unravelling Shared Pathways Linking Metabolic Syndrome, Mild Cognitive Impairment, Dementia, and Sarcopenia
Authors: Ceccarelli Ceccarelli Daniela , Solerte Sebastiano Bruno
Published: February 2025

Background: Aging is characterized by shared cellular and molecular processes, and aging-related diseases might co-exist in a cluster of comorbidities, particularly in vulnerable individuals whose phenotype meets the criteria for frailty. Whilst the multidimensional definition of frailty is still controversial, there is an increasing understanding of the common pathways linking metabolic syndrome, cognitive decline, and sarcopenia, frequent conditions in frail elderly patients. Methods: We performed a systematic search in the electronic databases Cochrane Library and PubMed and included preclinical studies, cohort and observational studies, and trials. Discussion: Metabolic syndrome markers, such as insulin resistance and the triglyceride/HDL C ratio, correlate with early cognitive impairment. Insulin resistance is a cause of synaptic dysfunction and neurodegeneration. Conversely, fasting and fasting-mimicking agents promote neuronal resilience by enhancing mitochondrial efficiency, autophagy, and neurogenesis. Proteins acting as cellular metabolic sensors, such as SIRT1, play a pivotal role in aging, neuroprotection, and metabolic health. In AD, β-amyloid accumulation and hyperphosphorylated tau in neurofibrillary tangles can cause metabolic reprogramming in brain cells, shifting from oxidative phosphorylation to aerobic glycolysis, similar to the Warburg effect in cancer. The interrelation of metabolic syndrome, sarcopenia, and cognitive decline suggests that targeting these shared metabolic pathways could mitigate all the conditions. Pharmacological interventions, including GLP-1 receptor agonists, metformin, and SIRT 1 inducers, demonstrated neuroprotective effects in animals and some preliminary clinical models. Conclusions: These findings encourage further research on the prevention and treatment of neurodegenerative diseases as well as the drug-repurposing potential of molecules currently approved for diabetes, dyslipidemia, and metabolic syndrome.
Two-Dimensional Simulation on the Critical Diameter of Particles in Asymmetric I-Shaped DLD Arrays
Authors: Wu Jiangbo , Yan Zihan , He Yongqing , Liu Jie , Lv Yao
Published: February 2025

Deterministic lateral displacement (DLD) is a passive particle separation method based on microfluidic technology, with its separation mechanism primarily relying on particle size differences. Therefore, the critical separation size is of great significance in the design of DLD devices. The geometric asymmetry of the pillar array design significantly influences fluid behavior and critical particle size variations. This study first analyzed particle motion characteristics through particle trajectory observation experiments within asymmetric microfluidic chips. Subsequently, a two-dimensional numerical simulation method was employed to investigate the effects of three different ratios of lateral gap size to downstream gap size (Gx:Gy) on particle trajectories and flow field distribution. The results indicate that as Gx:Gy decreases, the upward flow rate gradually reduces, accompanied by changes in the flow field velocity distribution, causing particles to favor displacement mode. This study provides new theoretical foundations for the precise regulation of particle motion behavior and introduces novel insights for optimizing DLD device design.
A Novel Closed-Loop Single-Channel Time Division Multiplexing Detection Circuit for Hemispherical Resonator Gyroscope
Authors: Wang Qi , Xie Weinan , Xi Boqi , Li Hanshi , Yi Guoxing
Published: February 2025

The vector control method is applied to a whole angle hemispherical resonator gyroscope (HRG). The detection and control of the resonator vibration state are implemented using orthogonal X/Y channels. However, the performance of the HRG is limited by the asymmetry in the gain and phase delay of X/Y channels. To address these issues, a novel detection circuit is proposed. The circuit leverages the closed-loop characteristics to achieve symmetry and stability in the X/Y channel gain while simultaneously eliminating phase delays within the loop. Firstly, a closed-loop single-channel time division multiplexing circuit is designed to overcome the deficiencies of the traditional dual-channel circuit. Secondly, a model is developed to analyze the time division detection errors, and an improved demodulation method is proposed to mitigate detection errors. Lastly, experimental results demonstrate that the designed circuit successfully suppresses drift in both gain and phase delay within the loop, confirming the effectiveness of the proposed solution in enhancing the performance of the HRG.
Study of Charged Nanodroplet Deposition into Microcavity Through Many-Body Dissipative Particle Dynamics
Authors: Jin Yiwei , Chen Jiankui , Chen Wei , Yin Zhouping
Published: February 2025

For a near-eye display, a resolution of over 10,000 pixels per inch (PPI) for the display device is needed to eliminate the “screen door effect” and have better display quality. Electrohydrodynamic (EHD) printing techniques, which have the advantages of a high resolution, wide material applicability and flexibility in patterning, have been widely used in the printing of high-resolution structures. However, due to factors such as the extremely small size of the droplets, the electric charge, the electric field, and the unavoidable positioning error, various deposition defects can occur. For droplets at a nanoscale, the dynamic deposition process is hard to observe. The continuum hypothesis fails and the fluid cannot be described by the traditional Navier–Stokes equation. In this work, the behaviors of charged nanodroplet deposition into a microcavity in an electric field are studied. The many-body dissipative particle dynamics (MDPD) method is used to examine the deformation of the nanodroplet during the impact process at a mesoscale. The dynamic process of charged droplet deposition into a microcavity under an electric field is revealed. Strategies for failure-free printing are proposed by analyzing the influences of the impact speeds, positioning errors, charge levels and electric intensities on the out-of-pixel spread length. The relationship between the internal charge moves and the deformation of the charged droplet in the deposition process is first discussed. The spreading theory of charged droplet deposition into a microcavity with a positioning error is established by analyzing the Coulombic capillary number. Moreover, the printing parameter space that results in successful printing is acquired.
Effect of Lactococcus lactis JNU 534 Supplementation on the Performance, Blood Parameters and Meat Characteristics of Salmonella enteritidis Inoculated Broilers
Authors: Purnamasari Listya , dela Cruz Joseph F. , Cho Dae-Yeon , Lee Kwang-Ho , Cho Sung-Min , Chung Seung-Sik , Choi Yong-Jun , Yi Jun-Koo , Hwang Seong-Gu
Published: February 2025

Salmonellosis in broilers is a disease with considerable economic implications for the poultry industry. As a foodborne illness, it also poses a public health risk due to potential cross-contamination. Probiotics have been proposed as alternative feed additives aiming to enhance growth, livestock productivity, and overall health. This study investigated the dietary impact of Lactococcus lactis JNU 534 on growth performance, blood characteristics, internal organ weight, and meat quality in broilers inoculated with Salmonella enteritidis (SE). A total of 96 one-day-old Arbor Acres broiler chickens, comprising both sexes, were challenged with SE and randomly assigned into two treatment groups and housed in eight pens (four pens per each treatment, with 12 birds per pen). They were fed a commercial broiler diet for 35 days. The two dietary treatment groups consisted of a control group receiving commercial feed, and a treatment group receiving commercial feed supplemented with 0.3% L. lactis JNU 534. Probiotic supplementation significantly improved average body weight gain, feed efficiency, and carcass yield compared to the control group (p < 0.05). Notably, the abdominal fat pad was significantly reduced in the probiotics group (p < 0.05). Meat quality assessments revealed no significant differences between the groups in terms of meat pH, cooking loss, drip loss, and water-holding capacity. These findings suggest that L. lactis JNU 534 is a promising candidate to mitigate the negative effects of Salmonella on growth performance in commercial broiler farms, without adversely affecting health. Extending the research to other types of livestock could help confirm its wider use as an alternative to antibiotics.
Evaluating Interlaboratory Variability in Wastewater-Based COVID-19 Surveillance
Authors: Azzellino Arianna , Pellegrinelli Laura , Pedrini Ramon , Turolla Andrea , Bertasi Barbara , Binda Sandro , Castiglioni Sara , Cocuzza Clementina E. , Ferrari Fabio , Franzetti Andrea , Guiso Maria Giovanna , Losio Marina Nadia , Martinelli Marianna , Martines Antonino , Musumeci Rosario , Oliva Desdemona , Sandri Laura , Primache Valeria , Righi Francesco , Scarazzato Annalisa , Schiarea Silvia , Pariani Elena , Ammoni Emanuela , Cereda Danilo , Malpei Francesca
Published: February 2025

Wastewater-based environmental surveillance enables the monitoring of SARS-CoV-2 dynamics within populations, offering critical epidemiological insights. Numerous workflows for tracking SARS-CoV-2 have been developed globally, underscoring the need for interlaboratory comparisons to ensure data consistency and comparability. An inter-calibration test was conducted among laboratories within the network monitoring SARS-CoV-2 in wastewater samples across the Lombardy region (Italy). The test aimed to evaluate data reliability and identify potential sources of variability using robust statistical approaches. Three wastewater samples were analyzed in parallel by four laboratories using identical pre-analytical (PEG-8000-based centrifugation) and analytical processes (qPCR targeting N1/N3 and Orf-1ab). A two-way ANOVA framework within Generalized Linear Models was applied, and multiple pairwise comparisons among laboratories were performed using the Bonferroni post hoc test. The statistical analysis revealed that the primary source of variability in the results was associated with the analytical phase. This variability was likely influenced by differences in the standard curves used by the laboratories to quantify SARS-CoV-2 concentrations, as well as the size of the wastewater treatment plants. The findings of this study highlight the importance of interlaboratory testing in verifying the consistency of analytical determinations and in identifying the key sources of variation.
Improved Isolation of Ultra-High-Molecular-Weight Genomic DNA Suitable for Third-Generation Sequencing
Authors: Ova Ayşe Öykü , Joffre Enrique , Zandi Shafagh Reza , Assunção Mariana F. G. , Sidorov Roman Y. , Santos Lilia M. A. , Lauschke Volker M. , Römling Ute
Published: February 2025

Although a variety of protocols to isolate high-molecular-weight genomic DNA exist, the isolation and preservation of ultra-high-molecular-weight genomic DNA of sufficient quality and length for error-free third-generation sequencing remains challenging. Inspired by the isolation of high-molecular-weight DNA in agarose plugs suitable to be separated by pulsed-field gel electrophoresis, we report the construction of an incubation chamber for DNA isolation. The chamber is flanked by semi-permeable polycarbonate membranes of variable pore size for the selective diffusion of compounds and components, which allows efficient cell lysis and the subsequent isolation of ultra-high-molecular-weight genomic DNA without shearing. The designed experimental approach is simple, but effective in isolating high-quality ultra-long genomic DNA that is suitable for third-generation sequencing by Oxford Nanopore Technology from challenging bacterial samples. We envisage that genomic DNA isolation using the incubation chamber, or variations thereof, will facilitate the regular sequencing of megabasepair-long DNA fragments, with a multitude of applications in microbiology, immunology, oncology, paleontology and forensic science.
Microbiome and Microbiota Within Wineries: A Review
Authors: Aires Cristina , Maioto Rita , Inês António , Dias Albino Alves , Rodrigues Paula , Egas Conceição , Sampaio Ana
Published: February 2025

The main goal of this work is to review the winery’s microbiota, from the grape to the winery’s microbial niches (fermentation tanks, surfaces, air), and their risks to wine and human health. The impact of climate change on the winery microbiome and related challenges are also discussed. Microbial diversity in wineries depends on several factors, such as the grape variety and its ripeness, temperature, relative humidity and the diverse activities of the winemaking process. Winery surfaces and equipment allow the establishment of a microbial community that can impact wine quality, the health of winery workers and visitors and even wine consumers. In the context of climate change, changes in the sugar content, phenolic compounds and the profile of hexoses and amino acids are already evident. These changes interfere with the fermentation microbiota and the quality of the wines, which are more alcoholic and less acidic. Furthermore, periods of drought or heavy rain favor species associated with berry diseases, including some capable of producing mycotoxins or harmful biogenic amines. In order to understand the impact of these changes on microbial communities, the use of various techniques will be discussed, such as flow cytometry, fluorescence in situ hybridization (FISH), quantitative polymerase chain reaction (qPCR) and metagenomic methods.
Enhanced Luminescence and Thermal Stability in High Gd3+/Eu3+ Co-Doped Ba3Y4O9 Phosphors via Co-Precipitation Method
Authors: Zhu Dong , Wang Chunfeng , Wang Xiaohuai , Han Shun , Zeng Yuxiang , Fang Ming , Liu Wenjun , Zhu Deliang , Cao Peijiang , Lu Youming
Published: February 2025

The co-precipitation method was successfully used to synthesize Ba3(Y0.6−xGd0.4Eux)4O9 (0.01 ≤ x ≤ 0.09) phosphors with heavy Gd3+ doping, resulting in significantly enhanced thermal stability and luminescence performance. Structural analyses confirm that Gd3+ and Eu3+ ions substitute Y3+ in the lattice, causing lattice expansion and improving crystal asymmetry, which enhances Eu3+ emission. The incorporation of Gd3+ creates efficient energy transfer pathways to Eu3+ while suppressing non-radiative relaxation, leading to stable fluorescence lifetimes even at elevated temperatures. With a thermal activation energy of ~0.3051 eV, the Ba3(Y0.55Gd0.4Eu0.05)4O9 phosphor exhibits superior resistance to thermal quenching compared to Ba3(Y0.95Eu0.05)4O9 and many conventional red phosphors. Furthermore, the reduced color temperature and stable emission spectra across a wide temperature range highlight its potential for advanced lighting and display technologies in high-temperature environments.
Development of Superhydrophobic Reduced Graphene Oxide (rGO) for Potential Applications in Advanced Materials
Authors: Adotey Enoch , Kurbanova Aliya , Ospanova Aigerim , Ardakkyzy Aida , Toktarbay Zhexenbek , Kydyrbay Nazerke , Zhazitov Mergen , Nuraje Nurxat , Toktarbaiuly Olzat
Published: February 2025

Reduced graphene oxide (rGO) was synthesized by chemically reducing graphene oxide (GO) using a reducing agent. The product, rGO, showed excellent hydrophobicity, as indicated by its high-water contact angle, which was greater than 150°. Characterizations using Fourier-transform infrared (FTIR) spectroscopy, Raman spectroscopy, and X-ray diffraction (XRD) were used to analyze the composition and structural differences between GO and the superhydrophobic rGO material. Scanning electron microscopy (SEM) showed that GO particles exhibited a plate-like morphology with layers of stacked plates, while rGO displayed fewer stacks that show a more separated structure of layers. The increasing demand for superhydrophobic materials in advanced materials industries, due to their potential to enhance performance, durability, and safety, makes rGO a promising candidate for use in composite materials.
Oxygen Vacancies on Hydrated Anatase (101) Surfaces: Insights from Classical and Ab Initio Molecular Dynamics Simulations
Authors: Grote Fredrik , Lyubartsev Alexander
Published: February 2025

Hydrated anatase (101) titanium dioxide surfaces with oxygen vacancies have been studied using a combination of classical and ab initio molecular dynamics simulations. The reactivity of surface oxygen vacancies was investigated using ab initio calculations, showing that water molecules quickly adsorb to oxygen vacancy sites upon hydration. The oxygen vacancy then quickly reacts with the adsorbed water, forming a protonated bridging oxygen atom at the vacancy site and at a neighboring oxygen bridge. Ab initio simulations also revealed that this occurs via a short-lived hydronium ion intermediate. It was investigated how this reaction affects the structure and dynamics of water near the anatase surface. Classical molecular dynamics simulations of surfaces with and without oxygen vacancies showed that vacancies disrupt the second solvation shell, consisting of water molecules hydrogen bonded to the surface, thereby changing the local water density and diffusion as well as the binding modes for hydrogen bonding. Our findings support the hydroxylation of oxygen vacancies on anatase (101) surfaces, rather than stabilization by molecular adsorption or subsurface diffusion. The work gives new atomistic insight into water structure and surface chemistry on the catalytically relevant anatase (101) titanium dioxide surface.
Low-Crystallized Carbon as an Electron Mediator in g-C3N4/C/TiO2 for Enhancing Photocatalytic Degradation of Antibiotics
Authors: Shen Yijie , Liu Zhe , Bi Renke , Zhou Bianbian , Wang Yan , Liu Jialong , Wang Zhiyu , Han Bing
Published: February 2025

Photodegradation of antibiotics based on photocatalytic semiconductors is a promising option to alleviate water pollution. Despite its limitations, TiO2-based photocatalysts are still the most widely studied materials for pollutant degradation. In this work, a pomegranate-like g-C3N4/C/TiO2 nano-heterojunction was constructed using the hydrothermal–calcination method, consisting of interconnected small crystals with a dense structure and closely contacted interface. Low-crystallized carbon filled the gap between TiO2 and g-C3N4, forming a large interface. The local in-plane heterostructures generated by C/g-C3N4 are further improved for carrier transport. As expected, the optimal sample calcined at 300 °C (GTC-300) efficiently eliminated tetracycline hydrochloride (TC-HCl, 20 mg L−1), achieving a removal rate of up to 92.9% within 40 min under full-spectrum irradiation and 87.8% within 60 min under the visible spectrum (λ > 400 nm). The electron mediator, low-crystallized carbon, successfully promoted the formation of new internal electric fields via the widespread heterojunction interface, which accelerated the separation and migration of photogenerated carriers between g-C3N4 and TiO2. These results confirm that the g-C3N4/C/TiO2 nano-heterojunction exhibited outstanding photodegradation performance of TC-HCl. The electron mediator shows great potential in promoting carrier transfer and enhancing photocatalytic performance of heterogeneous photocatalysts in water treatment.
Effects of One-Year Menaquinone-7 Supplementation on Vascular Stiffness and Blood Pressure in Post-Menopausal Women
Authors: de Vries Femke , Bittner Rudolf , Maresz Katarzyna , Machuron François , Gåserød Olav , Jeanne Jean-François , Schurgers Leon J.
Published: February 2025

Background/Objectives: Post-menopausal women are at an increased risk of developing cardiovascular disease. Menaquinone-7 (MK-7) is a fat-soluble vitamin involved in coagulation and maintaining vascular health. The aim of the post hoc analysis of this one-year study is to investigate the effects of MK-7 supplementation on the vascular parameters in pre-, peri-, and post-menopausal women. Methods: In a clinical intervention trial (NCT02404519), a total of 165 women with a low vitamin K status received either 180 µg of MK-7 daily (n = 82) or a matching placebo (n = 83) for one year. Established vascular parameters were measured before and after one year of vitamin K2 supplementation. Pre-, peri-, and post-menopausal women were subdivided according to arterial stiffness, with a high b-stiffness index defined as being greater than the overall median of 9.83. Results: The post hoc analyses showed a significant decrease in desphospho-uncarboxylated matrix Gla protein (dp-ucMGP) plasma levels after MK-7 supplementation (pre/peri, p = 0.009; post, p < 0.001). MK-7 treatment significantly attenuated vascular stiffness in post-menopausal women (placebo +49.1% ± 77.4; MK-7 +9.4% ± 67.1; p = 0.035). Post-menopausal women with a high stiffness index showed significantly improved vascular markers after MK-7 treatment, e.g., a decreased blood pressure at brachialis (−3.0% ± 9.0; p = 0.007) and an increased distensibility coefficient (+13.3% ± 32.3; p = 0.040). Conclusions: Our results confirm that menopause affects vascular health status. Post-menopausal women with an increased stiffness benefit most from MK-7 supplementation, with a significantly improved blood pressure. Further research is needed to unravel the beneficial effects of MK-7 in post-menopausal women.
Latency Reversing Agents and the Road to a HIV Cure
Authors: Tioka Louis , Diez Rafael Ceña , Sönnerborg Anders , van de Klundert Maarten A. A.
Published: February 2025

HIV-1 infection cannot be cured due to the presence of HIV-1 latently infected cells. These cells do not produce the virus, but they can resume virus production at any time in the absence of antiretroviral therapy. Therefore, people living with HIV (PLWH) need to take lifelong therapy. Strategies have been coined to eradicate the viral reservoir by reactivating HIV-1 latently infected cells and subsequently killing them. Various latency reversing agents (LRAs) that can reactivate HIV-1 in vitro and ex vivo have been identified. The most potent LRAs also strongly activate T cells and therefore cannot be applied in vivo. Many LRAs that reactivate HIV in the absence of general T cell activation have been identified and have been tested in clinical trials. Although some LRAs could reduce the reservoir size in clinical trials, so far, they have failed to eradicate the reservoir. More recently, immune modulators have been applied in PLWH, and the first results seem to indicate that these may reduce the reservoir and possibly improve immunological control after therapy interruption. Potentially, combinations of LRAs and immune modulators could reduce the reservoir size, and in the future, immunological control may enable PLWH to live without developing HIV-related disease in the absence of therapy.
Immunomodulatory Effects of a Standardized Botanical Mixture Comprising Angelica gigas Roots and Pueraria lobata Flowers Through the TLR2/6 Pathway in RAW 264.7 Macrophages and Cyclophosphamide-Induced Immunosuppression Mice
Authors: Jang Seo-Yun , Song Hyeon-A , Park Min-Ji , Chung Kyung-Sook , Lee Jong Kil , Jang Eun Yeong , Sun Eun Mi , Pyo Min Cheol , Lee Kyung-Tae
Published: February 2025

Background: As the population ages, enhancing immune function is crucial to mitigating age-related physiological decline. Since immunostimulant drugs are known to have potential side effects, medicinal plants emerge as promising candidates offering a safer alternative. To leverage the advantages of medicinal plants with fewer side effects and develop a potent immune-enhancing agent, we investigated the efficacy of a novel immunomodulatory candidate derived from the combination of Angelica gigas and Pueraria lobata (CHL). Methods: In vitro, CHL was treated in RAW 264.7 macrophages at various time points, and the experiments conducted in the study were performed using ELISA, Western blot, and RT-qPCR analysis. In vivo, C57BL/6 mice were administrated CHL for 16 days (p.o.) and CTX on the three days (i.p.), and experiments were conducted with ELISA, western blot, RT-qPCR analysis, H&E staining, flow cytometry, gut microbiome, and correlation analysis. Results: In vitro, CHL has upregulated NO and cytokines expression, substantially enhancing the NF-κB and MAPK activation. Furthermore, CHL promoted the TAK1, TRAF6, and MyD88 via TLR2/6 signaling. In vivo, the CHL improved the reduced body weight and immune organs’ indices and recovered various cytokines expression, NK cell cytotoxicity activity, and immune cell population. CHL also improved the histological structure and tight junction markers, mucin-2, and TLR2/6 in the intestines of CTX-induced mice. Conclusions: Overall, CHL demonstrated immunostimulatory potential by enhancing immune responses and restoring immune function, suggesting its promise as a safe and effective immune-enhancing agent.
RETRACTED: El-Gowily et al. Tioconazole and Chloroquine Act Synergistically to Combat Doxorubicin-Induced Toxicity via Inactivation of PI3K/AKT/mTOR Signaling Mediated ROS-Dependent Apoptosis and Autophagic Flux Inhibition in MCF-7 Breast Cancer Cells. Pharmaceuticals 2021, 14, 254
Authors: El-Gowily Afnan H. , Loutfy Samah A. , Ali Ehab M. M. , Mohamed Tarek M. , Mansour Mohammed A.
Published: February 2025

The journal retracts the article titled “Tioconazole and Chloroquine Act Synergistically to Combat Doxorubicin-Induced Toxicity via Inactivation of PI3K/AKT/mTOR Signaling Mediated ROS-Dependent Apoptosis and Autophagic Flux Inhibition in MCF-7 Breast Cancer Cells [...]
Pharmacological Treatment of Ascites: Challenges and Controversies
Authors: Lai Jimmy Che-To , Dai Junlong , Liang Lilian Yan , Wong Grace Lai-Hung , Wong Vincent Wai-Sun , Yip Terry Cheuk-Fung
Published: February 2025

Ascites is the most common complication from cirrhosis related to portal hypertension and depicts the onset of hepatic decompensation. Ranging from uncomplicated to refractory ascites, the progression carries prognostic value by reflecting the deterioration of underlying cirrhosis and portal hypertension. Diuretics have been the mainstay of treatment to control ascites, but the side effects heighten when the dosage is escalated. Non-selective beta-blockers (NSBBs) are widely used nowadays to prevent hepatic decompensation and variceal hemorrhage. However, with worsening systemic vasodilation and inflammation when ascites progresses, patients on NSBBs are at risk of hemodynamic collapse leading to renal hypoperfusion and thus hepatorenal syndrome. Long-term albumin infusion was studied to prevent the progression of ascites. However, the results were conflicting. Sodium-glucose cotransporter-2 inhibitors are under investigation to control refractory ascites. With that, patients with refractory ascites may require regular large-volume paracentesis. With an aging population, more patients are put on anti-thrombotic agents and their risks in decompensated cirrhosis and invasive procedures have to be considered. In general, decompensated cirrhosis with ascites poses multiple issues to pharmacological treatment. In the present review, we discuss the challenges and controversies in the pharmacological treatment of ascites.
Fluorescent Rhein-Loaded Liposomes for In Vivo Biodistribution Study
Authors: Filipiuc Silviu Iulian , Simionescu Natalia , Stanciu Gabriela Dumitrița , Coroaba Adina , Marangoci Narcisa Laura , Filipiuc Leontina Elena , Pinteala Mariana , Uritu Cristina Mariana , Tamba Bogdan Ionel
Published: February 2025

Objectives: This work aimed to develop and investigate liposomes incorporating Rhein (Lip-Rh) into the liposomal membrane to enhance the compound’s water solubility and oral bioavailability. Methods: Liposomes were produced by the thin lipid film technique, with a phosphatidylcholine-to-cholesterol molar ratio of 5:1, dissolved in chloroform and methanol, and thereafter hydrated with ultrapure water and subjected to sonication. The resultant liposomes were studied from a physicochemical perspective using DLS, zeta potential, STEM, UV–Vis, and fluorescence spectroscopies, while oral bioavailability was assessed by fluorescence imaging. Additionally, cell viability assays were performed on tumour cells (MCF-7) in comparison to normal cells (HGFs). Results: The resultant nanoparticles exhibited relatively uniform sizes and narrow size distribution. In vivo fluorescence imaging studies performed on Wistar rats demonstrated significantly enhanced oral bioavailability for Lip-Rh, with rapid absorption into the bloodstream observed one hour after administration, in contrast to the free compound dissolved in vegetable oil. Cell viability assays demonstrated higher cytotoxicity of Lip-Rh towards MCF-7 cells compared to HGF cells, highlighting the selective therapeutic potential of the product. Moreover, we determined that the optimal dose of Rhein per kilogram of body weight, when encapsulated in liposomes, is approximately 2.5 times less than when Rhein is delivered in its unencapsulated form. Conclusions: Lip-Rh is a promising candidate for oncological treatments, presenting three key advantages: increased cytotoxicity towards tumour cells, protection of normal tissues, and the practicality of oral delivery. Additional investigation is required to explore its application in anticancer therapy, whether as monotherapy or as a complementary treatment.
Plasma-Activated Water Produced by a Moderately High Energy-Efficient 1-Liter Reactor: Effects on Germination and Growth of Tomato and Bell Pepper Plants
Authors: Ferreyra Matías G. , Caffaro María M. , Santamaría Brenda , Zilli Carla , Hernández Alejandra , Fina Brenda L. , Vélez Ada S. , Balestrasse Karina B. , Prevosto Leandro
Published: February 2025

Plasma-activated water (PAW) is a sustainable and innovative alternative for agriculture, especially in controlled environments like greenhouses. Tomato and pepper are key horticultural crops worldwide, with a considerable part of their production in greenhouses. This study examined the effects of PAW irrigation on seed germination, plant growth, and oxidative stress in tomato and bell pepper plants. PAW was activated for up to 15 min using a 1 L capacity plasma reactor based on a glow-type discharge in air with water-cathode. The concentration of nitrogen compounds and the energy efficiency of synthesis obtained with the reactor were moderately high (5.4 mM and 60 nmol/J, respectively). The most notable effects of PAW were observed in bell pepper. The germination percentage in bell pepper increased by up to 26%, while no significant effects were found in tomato seeds. PAW irrigation significantly promoted plant growth, with dry weight increasing by up to 61% in bell pepper and 42% in tomato. Lipid peroxidation results showed no oxidative damage in either crop. The biochemical analysis of antioxidant enzymes (catalase, superoxide dismutase, and guaiacol peroxidase) confirmed that plant defense systems responded adequately to PAW irrigation. These results highlight PAW’s potential as an innovative and eco-friendly alternative in agriculture.
Response of Maize (Zea mays L.) to Foliar-Applied Nanoparticles of Zinc Oxide and Manganese Oxide Under Drought Stress
Authors: Kathirvelan Perumal , Vaishnavi Sonam , Manivannan Venkatesan , Djanaguiraman M. , Thiyageshwari S. , Parasuraman P. , Kalarani M. K.
Published: February 2025

Maize (Zea mays L.) is an important crop grown for food, feed, and energy. In general, maize yield is decreased due to drought stress during the reproductive stages, and, hence, it is critical to improve the grain yield under drought. A field experiment was conducted with a split-plot design. The main factor was the irrigation regime viz. well-irrigated conditions and withholding irrigation from tasseling to grain filling for 21 days. The subplots include six treatments, namely, (i) the control (water spray), (ii) zinc oxide @ 100 ppm, (iii) manganese oxide @ 20 ppm, (iv) nZnO @ 100 ppm + nMnO @ 20 ppm, (v) Tamil Nadu Agricultural University (TNAU) Nano Revive @ 1.0%, and (vi) zinc sulfate 0.25% + manganese sulfate 0.25%. During drought stress, the anthesis–silking interval (ASI), chlorophyll a and b content, proline, starch, and carbohydrate fractions were recorded. At harvest, the grain-filling rate and duration, per cent green leaf area, and yield traits were recorded. Drought stress increased the proline (38.1%) and anthesis–silking interval (0.45 d) over the irrigated condition. However, the foliar application of ZnO (100 ppm) and nMnO (20 ppm) lowered the ASI and increased the green leaf area, leaf chlorophyll index, and proline content over water spray. The seed-filling rate (17%), seed-filling duration (11%), and seed yield (19%) decreased under drought. Nevertheless, the seed-filling rate (90%), seed-filling duration (13%), and seed yield (52%) were increased by the foliar spraying of nZnO (100 ppm) and nMnO (20 ppm) over water spray. These findings suggest that nZnO and nMnO significantly improve the grain yield of maize under drought stress conditions.
Effect of Hard-Segment Structure on the Properties of Polyurethane/Poly(Ethyl Methacrylate) Damping Composites
Authors: Ma Jinbao , Ma Chi , Long Risheng , Jiang Yan , Wang Xingjia , Liu Chang , Li Fan , Sin Lee Tin
Published: February 2025

Damping material performance influences the efficacy of vibration and noise reduction. However, traditional damping materials often have low damping peaks or narrow damping temperature ranges. In this study, a series of polyurethane (PU)/poly(ethylene methacrylate) (PEMA) composites were synthesised, in which the PU hard segments were varied using toluene diisocyanate (TDI), diphenylmethane diisocyanate (MDI), isophorone diisocyanate (IPDI), and hexamethylene diisocyanate. The soft segments comprised tetrahydrofuran homopolymer glycol. The influence of the hard-segment structure on the properties of the PU/PEMA composites was investigated by infrared spectroscopy, thermogravimetric analysis, dynamic mechanical thermal analysis, and other experimental methods. The performance mechanism was explored from a molecular perspective via integration with molecular dynamics simulations. The PU/PEMA material with IPDI hard segments comprised numerous microphase-separated structures and exhibited greater free volume, fuller molecular-chain movement, and the highest damping performance, with a loss factor of 0.56. The PU/PEMA composites synthesised with TDI and MDI hard segments exhibited better compatibility, with the MDI-PU/PEMA system exhibiting a higher hydrogen-bonding force. This material also exhibited a higher thermal stability, with an initial breakdown temperature of 287.87 °C. This study provides a basis for regulating and optimising the performance of PU-based damping materials.
In Vitro Evaluation and Clinical Effects of a Regenerative Complex with Non-Cross-Linked Hyaluronic Acid and a High-Molecular-Weight Polynucleotide for Periorbital Treatment
Authors: Abuyousif Hanadi Sami , Porcello Alexandre , Cerrano Marco , Marques Cíntia , Scaletta Corinne , Lourenço Kelly , Abdel-Sayed Philippe , Chemali Michèle , Raffoul Wassim , Hirt-Burri Nathalie , Applegate Lee Ann , Laurent Alexis E.
Published: February 2025

Skin aging is a complex and multifactorial process influenced by both intrinsic and extrinsic factors. The periorbital area of the face is particularly susceptible to premature aging signs due to its delicate skin structure, and is a major concern for many individuals. While hyaluronic acid (HA)-based dermal filler products are commonly used for periorbital rejuvenation, novel approaches to effectively locally address the visible signs of aging are available. This study aimed to investigate Innovyal Regenerative Action (IRA), an injectable polynucleotide–HA (PN-HA) regenerative complex designed for periocular prejuvenation. Firstly, PN-HA was compared to other commercially available HA-based dermbooster products (Profhilo®, Suisselle Cellbooster® Glow, and NCTF® 135 HA) in terms of rheological properties, in vitro antioxidant capacity, and total collagen production stimulation in human fibroblasts. Secondly, the clinical effects of the IRA PN-HA complex were evaluated in two case reports (monotherapy for periorbital prejuvenation). It was shown that the PN-HA complex outperformed its comparators in terms of relative rheological behavior (biophysical attributes normalized to polymer contents), intrinsic antioxidant activity (CUPRAC, FRAP, and ORAC assays), as well as total collagen level induction (72-h in vitro dermal fibroblast induction model). Generally, the results of this study provided mechanistic and preliminary clinical insights into the potential benefits of the IRA PN-HA complex for periocular cutaneous treatment. Overall, it was underscored that combining the structural support and regenerative properties of PN with the hydrating and volumizing effects of HA bares tangible potential for multifactorial skin quality enhancement and for periocular prejuvenation in particular.