bluuebunny/crossref_metadata_2025 huggingface dataset:
Quantifying the Carbon Sequestration Costs for Pinus elliottii Afforestation Project of China Greenhouse Gases Voluntary Emission Reduction Program: A Case Study in Jiangxi Province
Authors: Cao Xian-lei , Li Xiu-shan , Breeze Tom D.
Published: August 2020

Afforestation and reforestation (AR) is an important component of China’s Greenhouse Gases (GHG) Voluntary Emission Reduction Program, acting as a valuable nationwide carbon sink. Although a number of studies have explored the costs of creating a carbon sink, such an assessment is lacking for China’s GHG Voluntary Emissions Reduction Program. This study develops an economic carbon sink costs measurement model for the Pinus elliottii afforestation project, based on the land opportunity cost method, which incorporates carbon sink transaction costs and leakage costs. From this, an empirical analysis on the carbon sink costs and its sensitivity factors was conducted. The results show that, firstly, the carbon sequestration costs of afforestation projects are generally high, ranging from 44.2 Yuan/tCO2e to 425.4 Yuan/tCO2e with and without considering the benefits of wood, respectively. This is higher than the current average carbon sink price of 20 Yuan/tCO2e. Secondly, forestry carbon sink transaction costs have a positive impact on carbon sequestration costs, but the impact is weak. Thirdly, carbon sequestration costs are negatively affected by timber prices but positively influenced by increasing labor prices and discount rate, which is not conductive to the development of carbon sink afforestation projects. In order to strengthen role of forestry in combating climate change, the study holds that the government departments should take measures to reduce carbon sink transaction costs, establish and improve the forestry carbon sink compensation mechanism in the future, and encourage wood-processing companies to make technological innovations to produce and sell durable wood products. The project owners can explore multiple operating models to increase their revenue, including market and non-market benefits, when the carbon sink afforestation project has been launched according to relevant methodologies.
Short Tandem Repeats (STRs) as Biomarkers for the Quantitative Follow-Up of Chimerism after Stem Cell Transplantation: Methodological Considerations and Clinical Application
Authors: Navarro-Bailón Almudena , Carbonell Diego , Escudero Asunción , Chicano María , Muñiz Paula , Suárez-González Julia , Bailén Rebeca , Oarbeascoa Gillen , Kwon Mi , Díez-Martín José Luis , Martínez-Laperche Carolina , Buño Ismael
Published: August 2020

Chimerism refers to the relative proportion of donor and recipient DNA after hematopoietic stem cell transplantation (HSCT) and its quantitative follow-up is of great clinical utility in this setting. PCR of short tandem repeats (STR-PCR) constitutes the gold standard method for chimerism quantification, although more sensitive PCR techniques (such as qPCR) have recently arisen. We compared the sensitivity and the quantification capacity of both techniques in patient samples and artificial mixtures and demonstrated adequate performance of both methods, with higher sensitivity of qPCR and better quantification skills of STR-PCR. By qPCR, we then prospectively followed up 57 patients that were in complete chimerism (CC) by STR-PCR. Twenty-seven patients (59%) showed 0.1–1% recipient DNA in the bone marrow. Only 4 patients presented 0.1–1% recipient DNA in peripheral blood (PB), and one of them relapsed. Finally, by qPCR, we retrospectively studied the last sample that showed CC by STR-PCR prior to relapse in 8 relapsed patients. At a median of 59 days prior to relapse, six patients presented mixed chimerism by qPCR in PB. Since both approaches have complementary characteristics, we conclude that different techniques should be applied in different clinical settings and therefore propose a methodological algorithm for chimerism follow-up after HSCT.
Repeat Self-Harm Following Hospital-Presenting Intentional Drug Overdose among Young People—A National Registry Study
Authors: Daly Caroline , Griffin Eve , McMahon Elaine , Corcoran Paul , Webb Roger T. , Witt Katrina , Ashcroft Darren M. , Arensman Ella
Published: August 2020

Background: The incidence of hospital-presenting self-harm peaks among young people, who most often engage in intentional drug overdose (IDO). The risk of self-harm repetition is high among young people and switching methods between self-harm episodes is common. However, little is known about their patterns of repetition and switching following IDO. This study aimed to investigate repeat self-harm and method-switching following hospital-presenting IDO among young people. Methods: Data from the National Self-Harm Registry Ireland on hospital-presenting self-harm by individuals aged 10–24 years during 2009–2018 were examined. Cox proportional hazards regression models with associated hazard ratios (HRs), survival curves and Poisson regression models with risk ratios (RRs), were used to examine risk factors for repetition and method-switching. Results: During 2009–2018, 16,800 young people presented following IDO. Within 12 months, 2136 young people repeated self-harm. Factors associated with repetition included being male (HR = 1.13, 95% CI: 1.03–1.24), aged 10–17 years (HR = 1.29, 95% CI: 1.18–1.41), consuming ≥ 50 tablets (HR = 1.27, 95% CI: 1.07–1.49) and taking benzodiazepines (HR = 1.67, 95% CI: 1.40–1.98) or antidepressants (HR = 1.36, 95% CI: 1.18–1.56). The cumulative risk for switching method was 2.4% (95% CI: 2.2–2.7). Method-switching was most likely to occur for males (RR = 1.36; 95% CI: 1.09–1.69) and for those who took illegal drugs (RR = 1.63; 95% CI: 1.19–2.25). Conclusion: Young males are at increased risk of repeat self-harm and method-switching following IDO and the type and quantity of drugs taken are further indicators of risk. Interventions targeting IDO among young people are needed that ensure that mental health assessments are undertaken and which address access to drugs.
Promoting Undetectable Equals Untransmittable in Sub-Saharan Africa: Implication for Clinical Practice and ART Adherence
Authors: Thomford Nicholas Ekow , Mhandire Doreen , Dandara Collet , Kyei George B.
Published: August 2020

In the last decade, reliable scientific evidence has emerged to support the concept that undetectable viral loads prevent human immunodeficiency virus (HIV). Undetectable equals untransmissible (U = U) is a simple message that everyone can understand. The success of this concept depends on strict adherence to antiretroviral therapy (ART) and the attainment of suppressed viral loads (VLs). To achieve U = U in sub-Saharan Africa (SSA), poor adherence to ART, persistent low-level viremia, and the emergence of drug-resistant mutants are challenges that cannot be overlooked. Short of a cure for HIV, U = U can substantially reduce the burden and change the landscape of HIV epidemiology on the continent. From a public health perspective, the U = U concept will reduce stigmatization in persons living with HIV (PLWHIV) in SSA and strengthen public opinion to accept that HIV infection is not a death sentence. This will also promote ART adherence because PLWHIV will aim to achieve U = U within the shortest possible time. This article highlights challenges and barriers to achieving U = U and suggests how to promote the concept to make it beneficial and applicable in SSA. This concept, if expertly packaged by policy-makers, clinicians, health service providers, and HIV control programs, will help to stem the tide of the epidemic in SSA.
Predictors of Urinary Pyrethroid and Organophosphate Compound Concentrations among Healthy Pregnant Women in New York
Authors: Balalian Arin A. , Liu Xinhua , Siegel Eva Laura , Herbstman Julie Beth , Rauh Virginia , Wapner Ronald , Factor-Litvak Pam , Whyatt Robin
Published: August 2020

Our study aimed to investigate dietary and non-dietary predictors of exposure to pyrethroids, organophosphates pesticides and 2,4-D herbicide in two cohorts of pregnant women in New York City: 153 women from the Thyroid Disruption and Infant Development (TDID) cohort and 121 from the Sibling/Hermanos Cohort(S/H). Baseline data on predictors were collected from the women at time of recruitment. We used three different modeling strategies to address missing data due to biomarker values below the limit of detection (<LOD): (1) logistic regression models with biomarkers categorized as (<median, ≥median); (2) linear regression models, imputing the <LOD values with (LOD/√2); (3) regression models, considering <LOD values as left-censored. Generally, all three models identified similar predictors of exposure. We found that ethnicity, higher income and education predicted higher concentrations of most of the biomarkers in both cohorts. Mothers who consumed processed meat in the TDID cohort, and broiled, barbequed food or burgers in the S/H cohort, tended to have lower concentrations of organophosphates and 2,4-D. The choice of modeling led to a few different predictors identified, and the selection of modeling strategy should be based on the study question.
Access to an Electronic Health Record: A Polish National Survey
Authors: Bujnowska-Fedak Maria Magdalena , Wysoczański Łukasz
Published: August 2020

In Poland, as in many countries around the world, e-health services are becoming more and more popular. Obligatory e-sick leave was implemented, followed by e-prescriptions and e-referrals. Therefore, it is worth considering the introduction of a complete electronic health record (EHR) that can be accessed by doctors and patients. The main aim of the study is to find out whether patients want to have access to their EHRs and if they would agree to pay for such a service. The research was based on three surveys conducted among 1000 Polish adults in 2007, 2012, and 2018. The sample collection was carried out by the national opinion poll agency, with the use of computer-assisted telephone interviews. Over 60% of respondents were interested in the possibility of accessing EHRs in general, and almost 50% of them were ready to pay for it. Nevertheless, when analyzing all the year-on-year trends, they were subject to a gradual decrease. The youngest age group was the one most interested in EHRs, while the group comprising respondents in middle age was the one mostwilling to pay for it. There is still great potential in implementing EHRs on a bigger scale.
Examining Injury Severity of Pedestrians in Vehicle–Pedestrian Crashes at Mid-Blocks Using Path Analysis
Authors: Peng Haorong , Ma Xiaoxiang , Chen Feng
Published: August 2020

Walking is a sustainable mode of transport which has well established health and environmental benefits. Unfortunately, hundreds of thousands of pedestrians lose their lives each year over the world due to involvement in road traffic crashes, and mid-blocks witness a significant portion of pedestrian fatalities. This study examined the direct and indirect effects of various contributing factors on the pedestrian injury severity in vehicle–pedestrian crashes at mid-blocks. Data of vehicle–pedestrian crashes during 2002–2009 were extracted from the NASS-GES, with pre-crash behaviors and injury severity included. The SEM path analysis method was applied to uncover the inter-relationships between the pedestrian injury severity and various explanatory variables. Both the direct and indirect effects of these explanatory variables on the pedestrian injury severity were calculated based on the marginal effects in the multinomial and ordered logit models. The results indicate some variables including number of road lanes and the age of pedestrian have indirect impacts on the injury severity through influencing the pre-crash behaviors. Although most indirect effects are relatively small compared with the direct effects, the results in this study still provide some valuable information to improve the overall understanding of pedestrian injury severity at mid-blocks.
Nuclear P38: Roles in Physiological and Pathological Processes and Regulation of Nuclear Translocation
Authors: Maik-Rachline Galia , Lifshits Lucia , Seger Rony
Published: August 2020

The p38 mitogen-activated protein kinase (p38MAPK, termed here p38) cascade is a central signaling pathway that transmits stress and other signals to various intracellular targets in the cytoplasm and nucleus. More than 150 substrates of p38α/β have been identified, and this number is likely to increase. The phosphorylation of these substrates initiates or regulates a large number of cellular processes including transcription, translation, RNA processing and cell cycle progression, as well as degradation and the nuclear translocation of various proteins. Being such a central signaling cascade, its dysregulation is associated with many pathologies, particularly inflammation and cancer. One of the hallmarks of p38α/β signaling is its stimulated nuclear translocation, which occurs shortly after extracellular stimulation. Although p38α/β do not contain nuclear localization or nuclear export signals, they rapidly and robustly translocate to the nucleus, and they are exported back to the cytoplasm within minutes to hours. Here, we describe the physiological and pathological roles of p38α/β phosphorylation, concentrating mainly on the ill-reviewed regulation of p38α/β substrate degradation and nuclear translocation. In addition, we provide information on the p38α/β ′s substrates, concentrating mainly on the nuclear targets and their role in p38α/b functions. Finally, we also provide information on the mechanisms of nuclear p38α/b translocation and its use as a therapeutic target for p38α/β-dependent diseases.
Extracellular Vesicles Mediate Anti-Oxidative Response—In Vitro Study in the Ocular Drainage System
Authors: Lerner Natalie , Chen Itay , Schreiber-Avissar Sofia , Beit-Yannai Elie
Published: August 2020

The importance of extracellular vesicles (EVs) as signaling mediators has been emphasized for several pathways with only limited data regarding their role as protective messages during oxidative stress (OS). The ocular drainage system is unique by being continuously exposed to OS and having a one-way flow of the aqueous humor carrying EVs taking role in glaucoma disease. Here, we aimed to examine the ability of EVs derived from the non-pigmented ciliary epithelium (NPCE)—the aqueous humor producing cells exposed to OS—to deliver protecting messages to the trabecular meshwork (TM)—the aqueous humor draining cells—a process with significance to the pathophysiology of glaucoma disease. EVs extracted from media of NPCE cells exposed to non-lethal OS and their unstressed control were incubated with TM cells. The effects of EVs derived from oxidative stressed cells on the activation of the nuclear factor erythroid 2-related factor 2-Kelch-like ECH-associated protein 1 (Nrf2-Keap1), a major OS pathway, and of the Wnt pathway, known for its role in primary open-angle glaucoma, were evaluated. EVs derived from oxidized NPCE cells significantly protected TM cells from direct OS. The TM cells uptake of EVs from oxidized NPCE and their cytosolic Nrf2 levels were significantly higher at 8 h post-exposure. EVs derived from oxidized NPCE cells significantly attenuated Wnt protein expression in TM cells and activated major antioxidant genes as measured by qRT-PCR. TM cells exposed to EVs derived from oxidized NPCE cells exhibited significantly lower OS and higher super oxide dismutase and catalase activity. Finally, we were able to show that carbonylated proteins and products of oxidized protein are presented in significantly higher levels in EVs derived from oxidized NPCE cells, supporting their suggested role in the signaling process. We hypothesize that these findings may have implications beyond understanding the pathophysiology of glaucoma disease and that transmitting signals that activate the antioxidant system in target cells represent a broad response common to many tissues communication.
Lipophagy and Lipolysis Status in Lipid Storage and Lipid Metabolism Diseases
Authors: Kloska Anna , Węsierska Magdalena , Malinowska Marcelina , Gabig-Cimińska Magdalena , Jakóbkiewicz-Banecka Joanna
Published: August 2020

This review discusses how lipophagy and cytosolic lipolysis degrade cellular lipids, as well as how these pathway ys communicate, how they affect lipid metabolism and energy homeostasis in cells and how their dysfunction affects the pathogenesis of lipid storage and lipid metabolism diseases. Answers to these questions will likely uncover novel strategies for the treatment of aforementioned human diseases, but, above all, will avoid destructive effects of high concentrations of lipids—referred to as lipotoxicity—resulting in cellular dysfunction and cell death.
Biotransformation of Methoxyflavones by Selected Entomopathogenic Filamentous Fungi
Authors: Łużny Mateusz , Tronina Tomasz , Kozłowska Ewa , Dymarska Monika , Popłoński Jarosław , Łyczko Jacek , Kostrzewa-Susłow Edyta , Janeczko Tomasz
Published: August 2020

The synthesis and biotransformation of five flavones containing methoxy substituents in the B ring: 2′-, 3′-, 4′-methoxyflavones, 2′,5′-dimethoxyflavone and 3′,4′,5′-trimethoxyflavone are described. Strains of entomopathogenic filamentous fungi were used as biocatalysts. Five strains of the species Beauveria bassiana (KCh J1.5, J2.1, J3.2, J1, BBT), two of the species Beauveria caledonica (KCh J3.3, J3.4), one of Isaria fumosorosea (KCh J2) and one of Isaria farinosa (KCh KW 1.1) were investigated. Both the number and the place of attachment of the methoxy groups in the flavonoid structure influenced the biotransformation rate and the amount of nascent products. Based on the structures of products and semi-products, it can be concluded that their formation is the result of a cascading process. As a result of enzymes produced in the cells of the tested strains, the test compounds undergo progressive demethylation and/or hydroxylation and 4-O-methylglucosylation. Thirteen novel flavonoid 4-O-methylglucosides and five hydroxy flavones were isolated and identified.
Versatility of Induced Pluripotent Stem Cells (iPSCs) for Improving the Knowledge on Musculoskeletal Diseases
Authors: Sanjurjo-Rodríguez Clara , Castro-Viñuelas Rocío , Piñeiro-Ramil María , Rodríguez-Fernández Silvia , Fuentes-Boquete Isaac , Blanco Francisco J. , Díaz-Prado Silvia
Published: August 2020

Induced pluripotent stem cells (iPSCs) represent an unlimited source of pluripotent cells capable of differentiating into any cell type of the body. Several studies have demonstrated the valuable use of iPSCs as a tool for studying the molecular and cellular mechanisms underlying disorders affecting bone, cartilage and muscle, as well as their potential for tissue repair. Musculoskeletal diseases are one of the major causes of disability worldwide and impose an important socio-economic burden. To date there is neither cure nor proven approach for effectively treating most of these conditions and therefore new strategies involving the use of cells have been increasingly investigated in the recent years. Nevertheless, some limitations related to the safety and differentiation protocols among others remain, which humpers the translational application of these strategies. Nonetheless, the potential is indisputable and iPSCs are likely to be a source of different types of cells useful in the musculoskeletal field, for either disease modeling or regenerative medicine. In this review, we aim to illustrate the great potential of iPSCs by summarizing and discussing the in vitro tissue regeneration preclinical studies that have been carried out in the musculoskeletal field by using iPSCs.
TRPC and TRPV Channels’ Role in Vascular Remodeling and Disease
Authors: Martín-Bórnez Marta , Galeano-Otero Isabel , del Toro Raquel , Smani Tarik
Published: August 2020

Transient receptor potentials (TRPs) are non-selective cation channels that are widely expressed in vascular beds. They contribute to the Ca2+ influx evoked by a wide spectrum of chemical and physical stimuli, both in endothelial and vascular smooth muscle cells. Within the superfamily of TRP channels, different isoforms of TRPC (canonical) and TRPV (vanilloid) have emerged as important regulators of vascular tone and blood flow pressure. Additionally, several lines of evidence derived from animal models, and even from human subjects, highlighted the role of TRPC and TRPV in vascular remodeling and disease. Dysregulation in the function and/or expression of TRPC and TRPV isoforms likely regulates vascular smooth muscle cells switching from a contractile to a synthetic phenotype. This process contributes to the development and progression of vascular disorders, such as systemic and pulmonary arterial hypertension, atherosclerosis and restenosis. In this review, we provide an overview of the current knowledge on the implication of TRPC and TRPV in the physiological and pathological processes of some frequent vascular diseases.
Endoplasmic Reticulum Stress and Unfolded Protein Response in Neurodegenerative Diseases
Authors: Ghemrawi Rose , Khair Mostafa
Published: August 2020

The endoplasmic reticulum (ER) is an important organelle involved in protein quality control and cellular homeostasis. The accumulation of unfolded proteins leads to an ER stress, followed by an adaptive response via the activation of the unfolded protein response (UPR), PKR-like ER kinase (PERK), inositol-requiring transmembrane kinase/endoribonuclease 1α (IRE1α) and activating transcription factor 6 (ATF6) pathways. However, prolonged cell stress activates apoptosis signaling leading to cell death. Neuronal cells are particularly sensitive to protein misfolding, consequently ER and UPR dysfunctions were found to be involved in many neurodegenerative diseases including Alzheimer’s disease, Parkinson’s disease, amyotrophic lateral sclerosis and prions diseases, among others characterized by the accumulation and aggregation of misfolded proteins. Pharmacological UPR modulation in affected tissues may contribute to the treatment and prevention of neurodegeneration. The association between ER stress, UPR and neuropathology is well established. In this review, we provide up-to-date evidence of UPR activation in neurodegenerative disorders followed by therapeutic strategies targeting the UPR and ameliorating the toxic effects of protein unfolding and aggregation.
Tumor Necrosis Factor α Influences Phenotypic Plasticity and Promotes Epigenetic Changes in Human Basal Forebrain Cholinergic Neuroblasts
Authors: Guarnieri Giulia , Sarchielli Erica , Comeglio Paolo , Herrera-Puerta Erika , Piaceri Irene , Nacmias Benedetta , Benelli Matteo , Kelsey Gavin , Maggi Mario , Gallina Pasquale , Vannelli Gabriella Barbara , Morelli Annamaria
Published: August 2020

TNFα is the main proinflammatory cytokine implicated in the pathogenesis of neurodegenerative disorders, but it also modulates physiological functions in both the developing and adult brain. In this study, we investigated a potential direct role of TNFα in determining phenotypic changes of a recently established cellular model of human basal forebrain cholinergic neuroblasts isolated from the nucleus basalis of Meynert (hfNBMs). Exposing hfNBMs to TNFα reduced the expression of immature markers, such as nestin and β-tubulin III, and inhibited primary cilium formation. On the contrary, TNFα increased the expression of TNFα receptor TNFR2 and the mature neuron marker MAP2, also promoting neurite elongation. Moreover, TNFα affected nerve growth factor receptor expression. We also found that TNFα induced the expression of DNA-methylation enzymes and, accordingly, downregulated genes involved in neuronal development through epigenetic mechanisms, as demonstrated by methylome analysis. In summary, TNFα showed a dual role on hfNBMs phenotypic plasticity, exerting a negative influence on neurogenesis despite a positive effect on differentiation, through mechanisms that remain to be elucidated. Our results help to clarify the complexity of TNFα effects in human neurons and suggest that manipulation of TNFα signaling could provide a potential therapeutic approach against neurodegenerative disorders.
Epidemiologic, Phenotypic, and Structural Characterization of Aminoglycoside-Resistance Gene aac(3)-IV
Authors: Plattner Michel , Gysin Marina , Haldimann Klara , Becker Katja , Hobbie Sven N.
Published: August 2020

Aminoglycoside antibiotics are powerful bactericidal therapeutics that are often used in the treatment of critical Gram-negative systemic infections. The emergence and global spread of antibiotic resistance, however, has compromised the clinical utility of aminoglycosides to an extent similar to that found for all other antibiotic-drug classes. Apramycin, a drug candidate currently in clinical development, was suggested as a next-generation aminoglycoside antibiotic with minimal cross-resistance to all other standard-of-care aminoglycosides. Here, we analyzed 591,140 pathogen genomes deposited in the NCBI National Database of Antibiotic Resistant Organisms (NDARO) for annotations of apramycin-resistance genes, and compared them to the genotypic prevalence of carbapenem resistance and 16S-rRNA methyltransferase (RMTase) genes. The 3-N-acetyltransferase gene aac(3)-IV was found to be the only apramycin-resistance gene of clinical relevance, at an average prevalence of 0.7%, which was four-fold lower than that of RMTase genes. In the important subpopulation of carbapenemase-positive isolates, aac(3)-IV was nine-fold less prevalent than RMTase genes. The phenotypic profiling of selected clinical isolates and recombinant strains expressing the aac(3)-IV gene confirmed resistance to not only apramycin, but also gentamicin, tobramycin, and paromomycin. Probing the structure–activity relationship of such substrate promiscuity by site-directed mutagenesis of the aminoglycoside-binding pocket in the acetyltransferase AAC(3)-IV revealed the molecular contacts to His124, Glu185, and Asp187 to be equally critical in binding to apramycin and gentamicin, whereas Asp67 was found to be a discriminating contact. Our findings suggest that aminoglycoside cross-resistance to apramycin in clinical isolates is limited to the substrate promiscuity of a single gene, rendering apramycin best-in-class for the coverage of carbapenem- and aminoglycoside-resistant bacterial infections.
An In-Situ Assessment of Wood-in-Service Using Microwave Technologies, with a Focus on Assessing Hardwood Power Poles
Authors: Brodie Graham , Thanigasalam Deepan Babu , Farrell Peter , Kealy Allison , French John R. J. , Ahmed (Shiday) Berhan
Published: August 2020

Wooden power poles and their ongoing inspection represent a significant investment for most electrical power utilities. This study explored the potential for using microwave fields to non-invasively assess the state of hardwood power poles in a field experiment. Two strategies were assessed: 2.4 GHz microwave field transmission through the pole; and mutual coupling between antennae using a 10.525 GHz radar module applied to the surface of the pole. Both systems distinguished between sound hardwood poles and those which were compromised by decay and subterranean termite attack and infestation.
Efficacy of Minimum Application of Chlorfluazuron Baiting to Control Urban Subterranean Termite Populations of Coptotermes gestroi (Wasmann) (Blattodea: Rhinotermitidae)
Authors: Wan Umar Wan Ahmad Syahir , Ab Majid Abdul Hafiz
Published: August 2020

Termite infestations in urban areas are a serious problem because they cause negative economic effects, reduce the esthetic value of buildings, damage crops, and require household repairs. Chemical controls are the most common method used against subterranean termites, and baiting has emerged as one of the prominent control methods. The goal of this research was to determine the efficacy of termite baiting by treating one of six active termite stations (selective baiting) with chlorfluazuron baits to eradicate six populations of subterranean termites. This work shows that the placement of chlorfluazuron baits in one of the active stations was sufficient to destroy a colony that was interconnected with multiple chlorfluazuron-free stations. In general, it requires an average of 4–8 weeks for a quantity of less than 300 g of chlorfluazuron bait to remove a termite infestation at the study site.
Point-of-Care Assays Could Be Useful for Therapeutic Drug Monitoring of IBD Patients in a Proactive Strategy with Adalimumab
Authors: Cherry Mohamad , Dutzer Dominique , Nasser Yara , Berger Anne-Emmanuelle , Roblin Xavier , Paul Stephane
Published: August 2020

The objective of the study was to evaluate whether Point-of-Care (POC) assays are equivalent to ELISAs for measuring residual trough levels of adalimumab (ADA) in a cohort of Inflammatory Bowel Disease (IBD) patients. ADA trough levels obtained by POC assays were used to optimize patients in daily clinical practice. Different assays (three ELISAs (Enzyme-Linked ImmunoSorbent Assay) from two different suppliers and two POC assays) were compared to measure ADA trough levels in a first cohort of 31 IBD patients. All assays revealed a high correlation within the assays, ranging from 0.86 to 0.99. Cut-off values were always higher with ELISAs than with POC assays. Then, a small prospective clinical study with a second cohort of 37 IBD patients was performed to compare POC assays and ELISAs for their ability to optimize patients on the basis of the measured ADA trough levels. The use of a POC assay to monitor ADA trough levels did not improve the follow-up of patients with loss of response, as they were always optimized whatever their ADA residual rate. For patients in clinical remission, a POC assay can be useful in some clinical situations to maintain or de-escalate ADA doses according to the measured trough levels. In conclusion, different assays for ADA monitoring are quite equivalent. A POC assay could be only useful for a proactive strategy for asymptomatic patients with a sub-therapeutic dose of ADA, but new therapeutic thresholds need to be identified.
Antimicrobial Stewardship Program, COVID-19, and Infection Control: Spread of Carbapenem-Resistant Klebsiella Pneumoniae Colonization in ICU COVID-19 Patients. What Did Not Work?
Authors: Tiri Beatrice , Sensi Emanuela , Marsiliani Viola , Cantarini Mizar , Priante Giulia , Vernelli Carlo , Martella Lucia Assunta , Costantini Monya , Mariottini Alessandro , Andreani Paolo , Bruzzone Paolo , Suadoni Fabio , Francucci Marsilio , Cirocchi Roberto , Cappanera Stefano
Published: August 2020

The Italian burden of disease associated with infections due to antibiotic-resistant bacteria has been very high, largely attributed to Carbapenem-Resistant Klebsiella pneumoniae (CR-Kp). The implementation of infection control measures and antimicrobial stewardship programs (ASP) has been shown to reduce healthcare-related infections caused by multidrug resistance (MDR) germs. Since 2016, in our teaching hospital of Terni, an ASP has been implemented in an intensive care unit (ICU) setting, with the “daily-ICU round strategy” and particular attention to infection control measures. We performed active surveillance for search patients colonized by Carbapenem-Resistant Enterobacteriaceae (CRE). In March 2020, coronavirus disease 2019 (COVID-19) arrived and the same ICU was reserved only for COVID-19 patients. In our retrospective observational study, we analyzed the bimonthly incidence of CRE colonization patients and the incidence of CRE acquisition in our ICU during the period of January 2019 to June 2020. In consideration of the great attention and training of all staff on infection control measures in the COVID-19 era, we would have expected a clear reduction in CRE acquisition, but this did not happen. In fact, the incidence of CRE acquisition went from 6.7% in 2019 to 50% in March–April 2020. We noted that 67% of patients that had been changed in posture with prone position were colonized by CRE, while only 37% of patients that had not been changed in posture were colonized by CRE. In our opinion, the high intensity of care, the prone position requiring 4–5 healthcare workers (HCWs), equipped with personal protective equipment (PPE) in a high risk area, with extended and prolonged contact with the patient, and the presence of 32 new HCWs from other departments and without work experience in the ICU setting, contributed to the spread of CR-Kp in our ICU, determining an increase in CRE acquisition colonization.
Bacterial Colonization of Irrigation Fluid during Aseptic Revision Knee Arthroplasty
Authors: Fuchs Michael , Pumberger Matthias , Hommel Hagen , Perka Carsten , von Roth Philipp , Thiele Kathi
Published: August 2020

Surgical risk factors for periprosthetic joint infection (PJI) after total knee arthroplasty (TKA) are the subjects of ongoing research. It is unclear if there are specific locations of the surgical area that might act as a pathogen source. Due to the fact that bacterial replication occurs preferably under humid conditions, it was our aim to investigate if irrigation fluid reservoirs on the surgical covers are subject to bacterial colonization. We prospectively observed 40 patients with scheduled aseptic 1-stage TKA revision. At time intervals of 30 min, irrigation samples were tested for microbiological colonization. Additionally, the suction tip was investigated at the end of surgery. Overall, a bacterial detection rate of 25% was found (57/232 samples). Analysis for any positive microbial detection revealed pathogen findings of irrigation fluid in 41.7% of samples after 30 min with a constant increase up to 77.8% after 90 min. Twenty-three percent of suction tips showed bacterial colonization. Coagulase-negative staphylococci, accounting for the majority of PJI, were the predominant pathogens. After an average follow-up of 17 months, no PJI was confirmed. Despite the substantial bacterial load of irrigation fluid, PJI rates were not elevated. Nevertheless, we recommend that irrigation fluid reservoirs should be prevented and not withdrawn by suction.
Risks According to the Timing and Frequency of Hypotension Episodes in Postanoxic Comatose Patients
Authors: Kim Yong Hwan , Lee Jae Hoon , Seo Jung In , Lee Dong Hoon , Kim Won Young , Lee Byung Kook
Published: August 2020

The aim of this study was to assess the risk of unfavorable outcomes according to the timing of hypotension episodes in cardiac arrest patients. This prospectively conducted multicenter observational study included 1373 out-of-hospital cardiac arrest patients treated with 33 °C targeted temperature management (TTM). Unfavorable neurological outcome and the incidence of complications were analyzed according to the timing of hypotension. Compared with hypotension before TTM initiation (adjusted hazard ratio (aHR) 1.51), hypotension within 6 h after TTM initiation was associated with an increased risk of unfavorable neurologic outcome (aHR 1.693), and after 24 h of TTM, was connected with decreased risk (aHR 1.277). The risk of unfavorable neurological outcome was gradually reduced over time after TTM initiation. Hypotension, persisting both before and during TTM, demonstrated a greater risk (aHR 2) than transient hypotension (aHR 1.265). Hypotension was correlated with various complications. Differences in lactate levels were persistent, regardless of the initial fluid therapy (p < 0.001). Hypotension showed a strong correlation with unfavorable neurological outcome, especially in the early phase after TTM initiation, and complications. It is essential to manage hypotension that occurs at the beginning of TTM initiation to recover cerebral function in cardiac arrest patients.
Joint Distribution of the Wave Crest and Its Associated Period for Nonlinear Random Waves of Finite Bandwidth
Authors: Cho Yong Jun
Published: August 2020

The theoretical treatment of statistical properties relevant to nonlinear random waves of finite bandwidth, such as the joint distribution of wave crest and its associated wave period, is an overdue task hampered by the complicated form of the analytical model for sea surface elevation. In this study, we first derive the wave crest distribution based on the simplified version of the Longuet-Higgins’ wave model and proceed to derive the joint distribution of the wave crest and its associated period, and the conditional wave period distribution with a given wave crest, which are of great engineering value. It is shown that the bandwidth of the wave spectrum has a significant influence on the crest distribution, and the significant wave crest is getting larger in an increasing manner as nonlinearity is increased as expected. It also turns out that the positive correlation of wave crest height with its associated period is extended to more massive waves as nonlinearity is enhanced contrary to the general perception in the coastal engineering community that the wave crest is a statistically independent random process with wave period over large waves. The peak period decreases due to the destructive interference of second-order free harmonics.
Beyond Calendars and Maps: Rethinking Time and Space for Effective Knowledge Governance in Protected Areas
Authors: Múnera-Roldán Claudia , Roux Dirk , Colloff Matthew , van Kerkhoff Lorrae
Published: August 2020

Protected area managers rely on relevant, credible, and legitimate knowledge. However, an increase in the rate, extent, severity, and magnitude of the impacts of drivers of change (e.g., climate change, altered land use, and demand for natural resources) is affecting the response capacity of managers and their agencies. We address temporal aspects of knowledge governance by exploring time-related characteristics of information and decision-making processes in protected areas. These areas represent artefacts where the past (e.g., geological periods and evolutionary processes), the present (e.g., biodiversity richness), and the future (e.g., protection of ecosystem services for future generations) are intimately connected and integrated. However, temporal horizons linked with spatial scales are often neglected or misinterpreted in environmental management plans and monitoring programs. In this paper, we present a framework to address multi-dimensional understandings of knowledge-based processes for managing protected areas to guide researchers, managers, and practitioners to consider temporal horizons, spatial scales, different knowledge systems, and future decisions. We propose that dealing with uncertain futures starts with understanding the knowledge governance context that shapes decision-making processes, explicitly embracing temporal dimensions of information in decision-making at different scales. We present examples from South Africa and Colombia to illustrate the concepts. This framework can help to enable a reflexive practice, identify pathways or transitions to enable actions and connect knowledge for effective conservation of protected areas.
Current Research Landscape of Marine-Derived Anti-Atherosclerotic Substances
Authors: Cao Qi , Zhao Jiarui , Xing Maochen , Xiao Han , Zhang Qian , Liang Hao , Ji Aiguo , Song Shuliang
Published: August 2020

Atherosclerosis is a chronic disease characterized by lipid accumulation and chronic inflammation of the arterial wall, which is the pathological basis for coronary heart disease, cerebrovascular disease and thromboembolic disease. Currently, there is a lack of low-cost therapeutic agents that effectively slow the progression of atherosclerosis. Therefore, the development of new drugs is urgently needed. The research and development of marine-derived drugs have gained increasing interest from researchers across the world. Many marine organisms provide a rich material basis for the development of atherosclerotic drugs. This review focuses on the latest technological advances in the structures and mechanisms of action of marine-derived anti-atherosclerotic substances and the challenges of the application of these substances including marine polysaccharides, proteins and peptides, polyunsaturated fatty acids and small molecule compounds. Here, we describe the theoretical basis of marine biological resources in the treatment of atherosclerosis.
Strengthening of Nanocrystalline Al with Al3Zr Core-Shell Structure
Authors: Janovszky Dora
Published: August 2020

High-density Al-based composites reinforced with ten-wt.% recycled nanocrystalline CuZrAgAl particles have been fabricated by mechanical milling, cold- and hot-pressing. The microstructures, phase transformations, and mechanical properties of the mixed powder and sintered samples were investigated. After milling in a ball mill for 30 h, the microhardness of the mixed powder increases to 301 ± 31 HV0.01 and 222 ± 10 HV0.01 without and with ethanol milling, respectively. On account of the interdiffusion, the melting temperature of mixed powder reduces to 574 ± 5.0 °C and 627.5 ± 6.5 °C after 30 h milling. The study showed that the reinforcing particles are homogeneously distributed in the sintered nanocrystalline Al-based composites. During the hot-pressing, a shell zone forms at the interface of reinforcing particles during hot pressing after high energy milling with a minimum of ten hours milling time. This shell zone consists of Al3Zr (D023) phase. The coarsening resistant core-shell structure and grain refinement greatly improve mechanical properties. The compression strength at room temperature varies between 650 and 800 MPa at room temperature and is 380 MPa at 400 °C for the composite containing ten-wt.% of the Cu-Zr-based amorphous-nanocrystalline phases. The Brinell hardness of the sintered composite is 329 HB.
Effect of V2O5 Additive on Micro-Arc Oxidation Coatings Fabricated on Magnesium Alloys with Different Loading Voltages
Authors: Wang Zhanying , Ma Ying , Wang Yushun
Published: August 2020

Effect of V2O5 additive in silicate-containing electrolyte on AZ91D magnesium alloys treated by micro-arc oxidation (MAO) technology under different loading voltages was investigated. The results showed that vanadium was well up-taken into the coating chemically. Moreover, a new phase of MgV2O4 with spinel structure was obtained in MAO coatings due to V2O5 added into the electrolyte. The MgV2O4 phase was responsible for the coatings exhibiting brown color and also was beneficial to improving the anti-corrosion property. In spotting tests, the corrosion resistances of coatings prepared under the high voltage are about 6–9 times higher than those of the low voltage because of the thicker coatings of the former. In potentiodynamic polarization tests, the coatings’ corrosion resistances were improved with the addition of V2O5, which was more significant under the low voltage than that under the high voltage. When the concentration of V2O5 was 0.2 g/L, the corrosion current density of the coating was the lowest, which means that the coating’s corrosion resistance under the low voltage is the best. Hence, it is necessary to carry out targeted design of the coating’s microstructure according to the different applications.
Optimization Design of a Small-Sized Cruciform Specimen for Biaxial Fatigue Testing
Authors: Lu Zheng , Zhao Jia-Yu , Zhou Chang-Yu , He Xiao-Hua
Published: August 2020

The in-plane biaxial specimen can well reflect the complex stress state of sheet metal. However, there is no standard for small-sized specimens used in the low-power biaxial fatigue testing machine. The main goal of this paper is to apply the finite element method and orthogonal experiment method to design the cruciform specimen, considering the influence of three main parameters including the diameter of the central semispherical thinning area, the minimum thickness of the center and the arm thickness of the specimen. According to the central strain dispersion coefficient and the strain concentration coefficient proposed in this paper, we ensured that the distribution of strain in the gauge area is uniform and the strain it at its maximum value at the same time. The optimized specimen is verified by a biaxial fatigue test with the digital image correlation (DIC) technique. It is found that the fatigue crack appears in the center region, which proves that the optimized specimen can be effectively used for biaxial fatigue test.
Thermomechanical Simulations of Residual Stresses and Distortion in Electron Beam Melting with Experimental Validation for Ti-6Al-4V
Authors: M. Abdullah Fawaz , Anwar Saqib , Al-Ahmari Abdulrahman
Published: August 2020

Electron beam melting (EBM) is a relatively new process in three-dimensional (3D) printing to enable rapid manufacturing. EBM can manufacture metallic parts with thin walls, multi-layers, and complex internal structures that could not otherwise be produced for applications in aerospace, medicine, and other fields. A 3D transient coupled thermomechanical finite element (FE) model was built to simulate the temperature distribution, distortion, and residual stresses in electron beam additive manufactured Ti-6Al-4V parts. This research enhances the understanding of the EBM-based 3D printing process to achieve parts with lower levels of residual stress and distortion and hence improved quality. The model used a fine mesh in the layer deposition zone, and the mesh size was gradually increased with distance away from the deposits. Then, elements are activated layer by layer during deposition according to the desired material properties. On the top surface, a Gaussian distributed heat flux is used to model the heat source, and the temperature-dependent properties of the powder and solid are also included to improve accuracy. The current simulation has been validated by comparing the FE distortion and temperature results with the experimental results and other reported simulation studies. The residual stress results calculated by the FE analysis were also compared with the previously reported simulation studies on the EBM process. The results showed that the finite element approach can efficiently and accurately predict the temperature field of a part during the EBM process and can easily be extended to other powder bed fusion processes.
Structural Defects in TiNi-Based Alloys after Warm ECAP
Authors: Lotkov Aleksandr , Baturin Anatoly , Kopylov Vladimir , Grishkov Victor , Laptev Roman
Published: August 2020

The microstructure, martensitic transformations and crystal structure defects in the Ti50Ni47.3Fe2.7 (at%) alloy after equal-channel angular pressing (ECAP, angle 90°, route BC, 1–3 passes at T = 723 K) have been investigated. A homogeneous submicrocrystalline (SMC) structure (grains/subgrains about 300 nm) is observed after 3 ECAP passes. Crystal structure defects in the Ti49.4Ni50.6 (at%) alloy (8 ECAP passes, angle 120°, BC route, T = 723 K, grains/subgrains about 300 nm) and Ti50Ni47.3Fe2.7 (at%) alloy with SMC B2 structures after ECAP were studied by positron lifetime spectroscopy at the room temperature. The single component with the positron lifetime τ1 = 132 ps and τ1 = 140 ps were observed for positron lifetime spectra (PLS) obtained from ternary and binary, correspondingly, annealed alloys with coarse-grained structures. This τ1 values correspond to the lifetime of delocalized positrons in defect-free B2 phase. The two component PLS were found for all samples exposed by ECAP. The component with τ2 = 160 ps (annihilation of positrons trapped by dislocations) is observed for all samples after 1–8 ECAP passes. The component with τ3 = 305 ps (annihilation of positrons trapped by vacancy nanoclusters) was detected only after the first ECAP pass. The component with τ3 = 200 ps (annihilation of positrons trapped by vacancies in the Ti sublattice of B2 structure) is observed for all samples after 3–8 ECAP passes.