bluuebunny/crossref_metadata_2025 huggingface dataset:
Role of Nitric Oxide in Gene Expression Regulation during Cancer: Epigenetic Modifications and Non-Coding RNAs
Authors: de la Cruz-Ojeda Patricia , Flores-Campos Rocío , Dios-Barbeito Sandra , Navarro-Villarán Elena , Muntané Jordi
Published: June 2021

Nitric oxide (NO) has been identified and described as a dual mediator in cancer according to dose-, time- and compartment-dependent NO generation. The present review addresses the different epigenetic mechanisms, such as histone modifications and non-coding RNAs (ncRNAs), miRNA and lncRNA, which regulate directly or indirectly nitric oxide synthase (NOS) expression and NO production, impacting all hallmarks of the oncogenic process. Among lncRNA, HEIH and UCA1 develop their oncogenic functions by inhibiting their target miRNAs and consequently reversing the inhibition of NOS and promoting tumor proliferation. The connection between miRNAs and NO is also involved in two important features in cancer, such as the tumor microenvironment that includes key cellular components such as tumor-associated macrophages (TAMs), cancer associated fibroblasts (CAFs) and cancer stem cells (CSCs).
Sustained Release of Insulin-Like Growth Factor-1 from Bombyx mori L. Silk Fibroin Delivery for Diabetic Wound Therapy
Authors: Lin Meng-Jin , Lu Mei-Chun , Chang Hwan-You
Published: June 2021

The goals of this study are to develop a high purity patented silk fibroin (SF) film and test its suitability to be used as a slow-release delivery for insulin-like growth factor-1 (IGF-1). The release rate of the SF film delivering IGF-1 followed zero-order kinetics as determined via the Ritger and Peppas equation. The release rate constant was identified as 0.11, 0.23, and 0.09% h−1 at 37 °C for SF films loaded with 0.65, 6.5, and 65 pmol IGF-1, respectively. More importantly, the IGF-1 activity was preserved for more than 30 days when complexed with the SF film. We show that the IGF-1-loaded SF films significantly accelerated wound healing in vitro (BALB/3T3) and in vivo (diabetic mice), compared with wounds treated with free IGF-1 and an IGF-1-loaded hydrocolloid dressing. This was evidenced by a six-fold increase in the granulation tissue area in the IGF-1-loaded SF film treatment group compared to that of the PBS control group. Western blotting analysis also demonstrated that IGF-1 receptor (IGF1R) phosphorylation in diabetic wounds increased more significantly in the IGF-1-loaded SF films group than in other experimental groups. Our results suggest that IGF-1 sustained release from SF films promotes wound healing through continuously activating the IGF1R pathway, leading to the enhancement of both wound re-epithelialization and granulation tissue formation in diabetic mice. Collectively, these data indicate that SF films have considerable potential to be used as a wound dressing material for long-term IGF-1 delivery for diabetic wound therapy.
Long-Lasting Growth Hormone Regulated by the Ubiquitin-Proteasome System
Authors: Kim Myung-Sun , Kim Kyunggon , Oh Su Kyung , Lee Gidae , Kim Jin-Ock , Li Lan , Park Jung-Hyun , Baek Kwang-Hyun
Published: June 2021

To increase the half-life of growth hormones, we proposed its long-lasting regulation through the ubiquitin-proteasome system (UPS). We identified lysine residues (K67, K141, and K166) that are involved in the ubiquitination of human growth hormone (hGH) using ubiquitination site prediction programs to validate the ubiquitination sites, and then substituted these lysine residues with arginine residues. We identified the most effective substituent (K141R) to prevent ubiquitination and named it AUT-hGH. hGH was expressed and purified in the form of hGH-His, and ubiquitination was first verified at sites containing K141 in the blood stream. Through the study, we propose that AUT-hGH with an increased half-life could be used as a long-lasting hGH in the blood stream.
Establishment of a Key Hidden Danger Factor System for Electric Power Personal Casualty Accidents Based on Text Mining
Authors: Lu Dan , Xu Changqing , Mi Chuanmin , Wang Yijing , Xu Xiangmin , Zhao Chufan
Published: June 2021

Based on actual safety management difficulties and needs, this paper aims to screen and extract the key accident potential factors of personal injuries and deaths within the electric power industry to provide a reference for electric power companies’ accident prevention effort. First, this document sorts out and analyzes all of the causes and influencing elements that may lead to the occurrence of electric personal injuries and deaths, based on which rough accident potential factors are initially identified and combined with the definition of accident potentials. Second, this paper mines and analyzes relevant accident report texts using text-mining technologies such as term count, word cloud, and term frequency–inverse document frequency (TF-IDF), and thus a system of key accident potential factors for personal injuries and deaths within the electric power industry, including three key factors (human, material, and management), is finally constructed. Workers’ habitual violation behavior, in particular, has a larger risk than other key accident potential components, implying that additional steps should be made to eradicate this type of critical accident potential in time.
Deep Learning Models for Colorectal Polyps
Authors: Bardhi Ornela , Sierra-Sosa Daniel , Garcia-Zapirain Begonya , Bujanda Luis
Published: June 2021

Colorectal cancer is one of the main causes of cancer incident cases and cancer deaths worldwide. Undetected colon polyps, be them benign or malignant, lead to late diagnosis of colorectal cancer. Computer aided devices have helped to decrease the polyp miss rate. The application of deep learning algorithms and techniques has escalated during this last decade. Many scientific studies are published to detect, localize, and classify colon polyps. We present here a brief review of the latest published studies. We compare the accuracy of these studies with our results obtained from training and testing three independent datasets using a convolutional neural network and autoencoder model. A train, validate and test split was performed for each dataset, 75%, 15%, and 15%, respectively. An accuracy of 0.937 was achieved for CVC-ColonDB, 0.951 for CVC-ClinicDB, and 0.967 for ETIS-LaribPolypDB. Our results suggest slight improvements compared to the algorithms used to date.
International Trade in the High-Tech Sector—Support or Obstacle to Start-Up Processes at the Macro Level in European Union Countries?
Authors: Gawel Aleksandra
Published: June 2021

High-tech internationalization touches on two aspects impacting entrepreneurship: openness of the economy and its level of innovativeness. Both of them might positively or negatively affect the process of new company creations, as suggested by the concept of entrepreneurial regime with creative destruction or by the concept of routinized regime with creative accumulation. The aim of the article is to assess the impact of high-tech internationalization on the start-up process of new company creation. Cluster analysis and panel modeling for European Union countries in 2009–2018 were conducted. The research results distinguish clusters of European Union countries with a different level of high-tech internationalization. The impact of high-tech internationalization on start-up rates is significant in the case of both clusters; however, it is stronger in the cluster of countries with a relatively higher level of high-tech international openness. The high-tech intra-EU import and extra-EU high-tech export negatively affect the rate of new enterprise creation in both clusters. Additionally, extra-EU import in clusters with a relatively higher level of high-tech internationalization also negatively impacts start-up rates. The only aspect supporting the start-up process is the level of intra-EU export in clusters with higher levels of high-tech internationalization. The results suggest that in European Union countries, creative accumulation is the dominant phenomenon.
Demarcation Technique and Geometric Analysis of Village Boundaries Resulting from Delineation of Land Areas (ujazd) in the Middle Ages in the Wielkopolska Region (Poland)
Authors: Oliskiewicz-Krzywicka Anna
Published: June 2021

The paper presents the genesis of village borders’ shape formed in the Middle Ages and presents rules according to which demarcations were made. The hypothesis that the borders formed in the Middle Ages had a shape similar to a circle or segment of a circle was accepted. This hypothesis was confirmed by geometric analysis of borders from that period. Geometric elements of the boundaries were calculated: types of arcs, their radii, lengths and central angles. The center of the newly granted area was analyzed, as this location played a major role in the delineation of the boundary. Accuracy of measurements was determined and influence of errors on the presented results was analyzed. Wielkopolska was chosen as the research area because natural conditions had no influence on the shape of the borders. The starting materials were topographic maps in the scale of 1:25,000 (Messtischblätter) from the years 1887–1890. The research confirmed the hypothesis of a circular shape of borders formed in the Middle Ages in the studied area. In the original allocations, the area took the shape of a circle, which was a specific pattern for the villages to be founded. Later, as the settlement developed, the villages took on the shapes of circle segments. Considering the demarcation technique, the accuracy of the boundary staking was high. The deviation from a perfect circle did not exceed approximately ± 60 m, and the length of the arc radius varied between 1500 and 2200 m. The historical village boundaries from medieval times, preserved to this day in their residual form, have a historic character due to their antiquity and should be protected.
Non-Ionic Surfactants for Stabilization of Polymeric Nanoparticles for Biomedical Uses
Authors: Cortés Hernán , Hernández-Parra Héctor , Bernal-Chávez Sergio A. , Prado-Audelo María L. Del , Caballero-Florán Isaac H. , Borbolla-Jiménez Fabiola V. , González-Torres Maykel , Magaña Jonathan J. , Leyva-Gómez Gerardo
Published: June 2021

Surfactants are essential in the manufacture of polymeric nanoparticles by emulsion formation methods and to preserve the stability of carriers in liquid media. The deposition of non-ionic surfactants at the interface allows a considerable reduction of the globule of the emulsion with high biocompatibility and the possibility of oscillating the final sizes in a wide nanometric range. Therefore, this review presents an analysis of the three principal non-ionic surfactants utilized in the manufacture of polymeric nanoparticles; polysorbates, poly(vinyl alcohol), and poloxamers. We included a section on general properties and uses and a comprehensive compilation of formulations with each principal non-ionic surfactant. Then, we highlight a section on the interaction of non-ionic surfactants with biological barriers to emphasize that the function of surfactants is not limited to stabilizing the dispersion of nanoparticles and has a broad impact on pharmacokinetics. Finally, the last section corresponds to a recommendation in the experimental approach for choosing a surfactant applying the systematic methodology of Quality by Design.
Design of Circular Composite Cylinders for Optimal Natural Frequencies
Authors: Serhat Gokhan
Published: June 2021

This study concerns optimizing the eigenfrequencies of circular cylindrical laminates. The stiffness properties are described by lamination parameters to avoid potential solution dependency on the initial assumptions of the laminate configurations. In the lamination parameter plane, novel response contours are obtained for the first and second natural frequencies as well as their difference. The influence of cylinder length, radius, thickness, and boundary conditions on the responses is investigated. The lamination parameters yielding the maximum response values are determined, and the first two mode shapes are shown for the optimum points. The results demonstrate that the maximum fundamental frequency points of the laminated cylinders mostly lie at the inner lamination parameter domain, unlike the singly curved composite panels. In addition, the second eigenfrequency shows a nonconvex response surface containing multiple local maxima for several cases. Moreover, the frequency difference contours appear as highly irregular, which is unconventional for free vibration responses.
Special Issue of the Manufacturing Engineering Society 2020 (SIMES-2020)
Authors: Rubio Eva María , Camacho Ana María
Published: June 2021

The Special Issue of the Manufacturing Engineering Society 2020 (SIMES-2020) has been launched as a joint issue of the journals “Materials” and “Applied Sciences”. The 17 contributions published in this Special Issue of Materials present cutting-edge advances in the field of Manufacturing Engineering, focusing on additive manufacturing and 3D printing; advances and innovations in manufacturing processes; sustainable and green manufacturing; manufacturing of new materials; manufacturing systems: machines, equipment and tooling; robotics, mechatronics and manufacturing automation; metrology and quality in manufacturing; Industry 4.0; design, modeling and simulation in manufacturing engineering. Among them, this issue highlights that the topic “advances and innovations in manufacturing processes” has collected a large number of contributions, followed by additive manufacturing and 3D printing; sustainable and green manufacturing; metrology and quality in manufacturing.
Prediction of Crack Resistance of LFSMA-13 with and without Anti-Rut Agent Using Parameters of FTIR Spectrum under Different Aging Degrees
Authors: Wu Xing , Kang Aihong , Wu Bangwei , Lou Keke , Fan Zhao
Published: June 2021

This paper aims to better analyze the crack resistance of lignin fiber reinforced SMA-13 (LFSMA-13) asphalt mixtures, with and without polymer anti-rut agent (ARA), under different aging degrees. IDEAL-CT test and Fourier transform infrared (FTIR) spectroscopy were utilized to analyze the relationships between the crack resistance of LFSMA-13, with and without ARA, and the parameters of the FTIR spectrum of the asphalt extracted from the test samples. A convenient testing method to predict the anti-crack ability of the mixtures in a road was also derived in this study. The test samples were prepared using the specifications listed by AASHTO. The fracture formation work (Winitial) and cracking index (CTIndex) in the IDEAL-CT test were adopted to reflect the cracking ability of the asphalt mixtures in both the crack formation stage and the crack propagation stage. The peak areas of the FTIR spectrum were utilized to reveal the chemical properties of the asphalt material inside the SMA-13 asphalt mixtures, with and without ARA under different aging degrees. Grey correlation analysis was adopted to choose the most suitable FTIR spectrum parameters to derive the prediction models of Winitial and CTIndex under different aging degrees. After conducting a series of tests, the results showed that the aging process could well affect the crack resistance of the test samples and the peak areas of the asphalt extracted from the mixtures. The FTIR parameters selected from the grey correlation analysis could be used to well predict the anti-crack ability of the asphalt mixtures.
Phase Structure Evolution of the Fe-Al Arc-Sprayed Coating Stimulated by Annealing
Authors: Chmielewski Tomasz , Chmielewski Marcin , Piątkowska Anna , Grabias Agnieszka , Skowrońska Beata , Siwek Piotr
Published: June 2021

The article presents the results of research on the structural evolution of the composite Fe-Al-based coating deposited by arc spray with initial low participation of in situ intermetallic phases. The arc spraying process was carried out by simultaneously melting two different electrode wires, aluminum and low alloy steel (98.6 wt.% of Fe). The aim of the research was to reach protective coatings with a composite structure consisting of a significant participation of FexAly as intermetallic phases reinforcement. Initially, synthesis of intermetallic phases took place in situ during the spraying process. In the next step, participation of FexAly fraction was increased through the annealing process, with three temperature values, 700 °C, 800 °C, and 900 °C. Phase structure evolution of the Fe-Al arc-sprayed coating, stimulated by annealing, has been described by means of SEM images taken with a QBSD backscattered electron detector and by XRD and conversion electron Mössbauer spectroscopy (CEMS) investigations. Microhardness distribution of the investigated annealed coatings has been presented.
Chemical Characterisation of Silanised Zirconia Nanoparticles and Their Effects on the Properties of PMMA-Zirconia Nanocomposites
Authors: Zidan Saleh , Silikas Nikolaos , Al-Nasrawi Suhad , Haider Julfikar , Alshabib Abdulrahman , Alshame Alshame , Yates Julian
Published: June 2021

Objectives: The objective of this study was to investigate the mechanical properties of high-impact (HI) heat-cured acrylic resin (PMMA) reinforced with silane-treated zirconia nanoparticles. Methods: Forty-five PMMA specimens reinforced with zirconia were fabricated and divided into three groups: Pure HI PMMA (control group), PMMA reinforced with 3 wt.% of non-silanised zirconia nanoparticles and PMMA reinforced with 3 wt.% of silanised zirconia nanoparticles. Silanised and non-silanised zirconia nanoparticles were analysed with Fourier Transform Infrared (FTIR) Spectroscopy. For measuring the flexural modulus and strength, a Zwick universal tester was used, and for surface hardness, a Vickers hardness tester were used. Furthermore, raw materials and fractured surfaces were analysed using Scanning Electron Microscopy (SEM). A one-way ANOVA test followed by a post-hoc Bonferroni test was employed to analyse the data. Results: The results showed that the mean values for flexural strength (83.5 ± 6.2 MPa) and surface hardness (20.1 ± 2.3 kg/mm2) of the group containing 3 wt.% treated zirconia increased significantly (p < 0.05) in comparison to the specimens in the group containing non-treated zirconia (59.9 ± 7.1 MPa; 15.0 ± 0.2 kg/mm2) and the control group (72.4 ± 8.6 MPa; 17.1 ± 0.9 kg/mm2). However, the group with silanised zirconia showed an increase in flexural modulus (2313 ± 161 MPa) but was not significantly different (p > 0.05) from the non-silanised group (2207 ± 252 MPa) and the control group (1971 ± 235 MPa). Conclusion: Silane-treated zirconia nano-filler improves the surface hardness and flexural strength of HI PMMA-zirconia nanocomposites, giving a potentially longer service life of the denture base.
Technology and Properties of Peripheral Laser-Welded Micro-Joints
Authors: Tofil Szymon , Danielewski Hubert , Witkowski Grzegorz , Mulczyk Krystian , Antoszewski Bogdan
Published: June 2021

This article presents the results of research on the technology and peripheral properties of laser-welded micro-couplings. The aim of this research was to determine the characteristics of properly made joints and to indicate the range of optimal parameters of the welding process. Thin-walled AISI 316L steel pipes with diameters of 1.5 and 2 mm used in medical equipment were tested. The micro-welding process was carried out on a SISMA LM-D210 Nd:YAG laser. The research methods used were macroscopic and microscopic analyses of the samples, and assessment of the distribution of elements in the weld, the distribution of microhardness and the tear strength of the joint. As a result of the tests, the following welding parameters are recommended: a pulse energy of 2.05 J, pulse duration of 4 ms and frequency of 2 Hz, beam focusing to a diameter of 0.4 mm and a rotation speed of 0.157 rad/s. In addition, the tests show good joint properties with a strength of more than 75% of the thinner pipe, uniform distribution of alloying elements and a complex dendritic structure characteristic of pulse welding.
Covalent Triazine Frameworks Based on the First Pseudo-Octahedral Hexanitrile Monomer via Nitrile Trimerization: Synthesis, Porosity, and CO2 Gas Sorption Properties
Authors: Wessely Isabelle D. , Schade Alexandra M. , Dey Subarna , Bhunia Asamanjoy , Nuhnen Alexander , Janiak Christoph , Bräse Stefan
Published: June 2021

Herein, we report the first synthesis of covalent triazine-based frameworks (CTFs) based on a hexanitrile monomer, namely the novel pseudo-octahedral hexanitrile 1,4-bis(tris(4′-cyano-phenyl)methyl)benzene 1 using both ionothermal reaction conditions with ZnCl2 at 400 °C and the milder reaction conditions with the strong Brønsted acid trifluoromethanesulfonic acid (TFMS) at room temperature. Additionally, the hexanitrile was combined with different di-, tri-, and tetranitriles as a second linker based on recent work of mixed-linker CTFs, which showed enhanced carbon dioxide captures. The obtained framework structures were characterized via infrared (IR) spectroscopy, elemental analysis, scanning electron microscopy (SEM), and gas sorption measurements. Nitrogen adsorption measurements were performed at 77 K to determine the Brunauer-Emmett-Teller (BET) surface areas range from 493 m2/g to 1728 m2/g (p/p0 = 0.01–0.05). As expected, the framework CTF-hex6 synthesized from 1 with ZnCl2 possesses the highest surface area for nitrogen adsorption. On the other hand, the mixed framework structure CTF-hex4 formed from the hexanitrile 1 and 1,3,5 tricyanobenzene (4) shows the highest uptake of carbon dioxide and methane of 76.4 cm3/g and 26.6 cm3/g, respectively, at 273 K.
Resistance to Fracture of Lithium Disilicate Feldspathic Restorations Manufactured Using a CAD/CAM System and Crystallized with Different Thermal Units and Programs
Authors: Abad-Coronel Cristian , Ordoñez Balladares Andrea , Fajardo Jorge I. , Martín Biedma Benjamín José
Published: June 2021

The aim of this study was to determine the resistance to fracture of feldspathic restorations with lithium disilicate and crystallized with different ovens and programs. Methods: Sixty monolithic restorations (LD) (EMAX CAD™ LT, Ivoclar-Vivadent™) were designed with the same parameters and milled with a CAD/CAM system (CEREC SW 5.1, CEREC MCXL, Dentsply-Sirona™, Bensheim). Each restoration was randomly assigned by randomization software (RANDNUM) to one of the three groups: (a) (NF) Oven P310 (Ivoclar, Vivadent) normal crystallization program, (b) (FF) Ivoclar P310 oven (Ivoclar-Vivadent™) rapid crystallization program, or (c) (SF) SpeedFire oven (Dentsply-Sirona™). Results: There were statistically significant differences between the groups (ANOVA, p < 0.05). The NF and FF groups showed the highest values of resistance to fracture, with statistically significant differences with the SF group. Conclusions: Using a furnace from the same dental company with predetermined programs from the material manufacturer, as well as using a predetermined program for rapid crystallization, has no effect on fracture resistance, and would save clinical time when performing ceramic restorations with lithium disilicate, while keeping their mechanical properties.
The Formation of Cr-Al Spinel under a Reductive Atmosphere
Authors: Shtyka Oleksandr , Maniukiewicz Waldemar , Ciesielski Radoslaw , Kedziora Adam , Shatsila Viktar , Sierański Tomasz , Maniecki Tomasz
Published: June 2021

In the present work, for the first time, the possibility of formation of CrAl2O4 was shown from the equimolar mixture of co-precipitated Al2O3 and Cr2O3 oxides under a reductive environment. The crystallographic properties of the formed compound were calculated using the DICVOL procedure. It was determined that it has a cubic crystal structure with space group Fd-3m and a unit cell parameter equal to 8.22(3) Å. The formed CrAl2O4 is not stable under ambient conditions and easily undergoes oxidation to α-Al2O3 and α-Cr2O3. The overall sequence of the phase transformations of co-precipitated oxides leading to the formation of spinel structure is proposed.
Comparison of the Performances of Different Computational Methods to Calculate the Electrochemical Stability of Selected Ionic Liquids
Authors: Paolone Annalisa , Brutti Sergio
Published: June 2021

The electrochemical stability windows (ESW) of selected ionic liquids have been calculated by comparing different computational approaches previously suggested in the literature. The molecular systems under study are based on di-alkyl imidazolium and tetra-alkyl ammonium cations coupled with two different imide anions (namely, bis-fluorosulfonyl imide and bis-trifluoromethyl sulfonyl imide), for which an experimental investigation of the ESW is available. Thermodynamic oxidation and reduction potentials have here been estimated by different models based on calculations either on single ions or on ionic couples. Various Density Functional Theory (DFT) functionals (MP2, B3LYP, B3LYP including a polarizable medium and empirical dispersion forces) were exploited. Both vertical and adiabatic transitions between the starting states and the oxidized or reduced states were considered. The approach based on calculations on ionic couples is not able to reproduce the experimental data, whatever the used DFT functional. The best quantitative agreement is obtained by calculations on single ions when the MP2 functional in vacuum is considered and the transitions between differently charged states are vertical (purely electronic without the relaxation of the structure). The B3LYP functional underestimates the ESW. The inclusion of a polar medium excessively widens the ESW, while a large shrinkage of the ESW is obtained by adopting an adiabatic transition scheme instead of a vertical transition one.
Minimal Optimized Effective Potentials for Density Functional Theory Studies on Excited-State Proton Dissociation
Authors: Partovi-Azar Pouya , Sebastiani Daniel
Published: June 2021

Recently, a new method [P. Partovi-Azar and D. Sebastiani, J. Chem. Phys. 152, 064101 (2020)] was proposed to increase the efficiency of proton transfer energy calculations in density functional theory by using the T1 state with additional optimized effective potentials instead of calculations at S1. In this work, we focus on proton transfer from six prototypical photoacids to neighboring water molecules and show that the reference proton dissociation curves obtained at S1 states using time-dependent density functional theory can be reproduced with a reasonable accuracy by performing T1 calculations at density functional theory level with only one additional effective potential for the acidic hydrogens. We also find that the extra effective potentials for the acidic hydrogens neither change the nature of the T1 state nor the structural properties of solvent molecules upon transfer from the acids. The presented method is not only beneficial for theoretical studies on excited state proton transfer, but we believe that it would also be useful for studying other excited state photochemical reactions.
Relative Efficacy of Weight Management, Exercise, and Combined Treatment for Muscle Mass and Physical Sarcopenia Indices in Adults with Overweight or Obesity and Osteoarthritis: A Network Meta-Analysis of Randomized Controlled Trials
Authors: Chu Shu-Fen , Liou Tsan-Hon , Chen Hung-Chou , Huang Shih-Wei , Liao Chun-De
Published: June 2021

Aging and osteoarthritis are associated with high risk of muscle mass loss, which leads to physical disability; this loss can be effectively alleviated by diet (DI) and exercise (ET) interventions. This study investigated the relative effects of different types of diet, exercise, and combined treatment (DI+ET) on muscle mass and functional outcomes in individuals with obesity and lower-limb osteoarthritis. A comprehensive search of online databases was performed to identify randomized controlled trials (RCTs) examining the efficacy of DI, ET, and DI+ET in patients with obesity and lower-extremity osteoarthritis. The included RCTs were analyzed through network meta-analysis and risk-of-bias assessment. We finally included 34 RCTs with a median (range/total) Physiotherapy Evidence Database score of 6.5 (4–8/10). DI plus resistance ET, resistance ET alone, and aerobic ET alone were ranked as the most effective treatments for increasing muscle mass (standard mean difference (SMD) = 1.40), muscle strength (SMD = 1.93), and walking speed (SMD = 0.46). Our findings suggest that DI+ET is beneficial overall for muscle mass in overweight or obese adults with lower-limb osteoarthritis, especially those who are undergoing weight management.
Association between Bone Mineral Density and Fat Mass Independent of Lean Mass and Physical Activity in Women Aged 75 or Older
Authors: Mathieu Marie , Guillot Pascale , Riaudel Typhaine , Boureau Anne-Sophie , Chapelet Guillaume , Brouessard Céline , de Decker Laure , Berrut Gilles
Published: June 2021

(1) Osteoporosis and sarcopenia are frequent pathologies among the geriatric population. The interlink between these two diseases is supported by their common pathophysiology. The aim is to explore the relationship between bone mineral density (BMD) and body composition in women aged 75 or older. (2) From January 2016 to December 2019, women aged 75 or older of Caucasian ethnicity, who were addressed to perform a biphoton absorptiometry (DXA), were included in this observational study. Femoral neck T-score, lean mass, fat mass, and physical performances were measured. (3) The mean age of 101 patients included was 84.8 (±4.9) years old. Osteoporosis was present in 72% of patients. According to EWGSOP criteria, 37% of patients were sarcopenic. Osteosarcopenia was present in 34% of patients. The femoral neck T-score was significantly associated with fat mass (β = 0.02, 95% CI (0.01; 0.03), p < 0.05) in multivariable analysis. Osteosarcopenic patients had significantly lower fat mass (16.2 kg (±6.8) vs. 23.1 kg (±10.8), p < 0.001) and body mass index (BMI) (20.7 kg/m2 (±2.8) vs. 26.7 kg/m2 (±5.6), p < 0.001). (4) In postmenopausal women, fat mass is estimated to provide hormonal protection. While osteosarcopenia is described as a lipotoxic disease, fat mass and BMI would appear to protect against the risk of osteosarcopenia. This raises questions about the relevance of BMI and DXA.
Influence of Algae Supplementation on the Concentration of Glutathione and the Activity of Glutathione Enzymes in the Mice Liver and Kidney
Authors: Świderska-Kołacz Grażyna , Jefimow Małgorzata , Klusek Jolanta , Rączka Norbert , Zmorzyński Szymon , Wojciechowska Anna , Stanisławska Iwona , Łyp Marek , Czerwik-Marcinkowska Joanna
Published: June 2021

Algae are potential and natural source of long-chain polyunsaturated fatty acids like eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). The diatom Pinnularia borealis accumulates high levels of EPA and may be considered as a source for commercial production of dietary supplements. In this study we asked the question whether diet supplementation with P. borealis may augment antioxidant defense and ameliorate risk factors for cardiovascular diseases. We fed mice (Mus musculus) with lyophilized diatom solutions of different concentrations (1%, 3%, and 5%) for 7 days. Then we measured glutathione content and the activity of glutathione redox system enzymes, total cholesterol and triacylglycerol concentrations, and malondialdehyde concentration in the liver and kidney. We found that cholesterol and triacylglycerol concentrations in the liver and kidneys were the lowest in mice who were fed with the highest concentration of Pinnularia borealis, suggesting protective properties of algae. Additionally, the lowest concentration of Pinnularia borealis was sufficient to improve antioxidant capacity. Our results suggest that P. borealis may be used as a source for dietary supplements rich in EPA, but the amount supplied to the organism should be limited.
Association of Trimethylamine N-Oxide (TMAO) with the Clinical Severity of Hidradenitis Suppurativa (Acne Inversa)
Authors: Barrea Luigi , Muscogiuri Giovanna , Pugliese Gabriella , de Alteriis Giulia , Maisto Maria , Donnarumma Marianna , Tenore Gian Carlo , Colao Annamaria , Fabbrocini Gabriella , Savastano Silvia
Published: June 2021

In this case-control, cross-sectional, observational study, we evaluated circulating trimethylamine n-oxide (TMAO) levels, a gut-derived metabolite associated with inflammation and cardiometabolic risk, in patients with hidradenitis suppurativa (HS), a highly disabling inflammatory skin disease associated with an elevated prevalence of comorbidities, especially cardiovascular and metabolic diseases. In this study, we enrolled 35 naive-treatment patients with HS and 35 controls, matched for sex, age, and body mass index (BMI). HS Sartorius score was 49.0 (33.0–75.0), while according to the Harley system 12 and 23 patients presented grade 1 and grade 2 severity, respectively. HS patients had a lower adherence to the Mediterranean diet (MD) (p = 0.002), lower phase angle (PhA) (p < 0.001), and higher circulating TMAO levels (p < 0.001) than the control group. HS patients with grade 2 rather than grade 1 of Harley grade severity showed a higher BMI (p = 0.007), waist circumference (p = 0.016), total energy intake (p = 0.005), and lower PhA (p < 0.001) and adherence to the MD (p = 0.003). Of interest, patients with Hurley grade 2 of severity exhibited higher circulating TMAO levels (p < 0.001) compared to grade 1. Circulating TMAO levels showed a positive correlation with HS Sartorius score even after adjustment for confounding covariates, including BMI, waist circumference, adherence to the MD, total energy intake, and PhA (r = 0.570, p = 0.001). Using a linear regression model, circulating TMAO levels and PhA were the main predictors of the clinical severity of HS.
The Hunger Games: Homeostatic State-Dependent Fluctuations in Disinhibition Measured with a Novel Gamified Test Battery
Authors: Voigt Katharina , Giddens Emily , Stark Romana , Frisch Emma , Moskovsky Neda , Kakoschke Naomi , Stout Julie C. , Bellgrove Mark A. , Andrews Zane B. , Verdejo-Garcia Antonio
Published: June 2021

Food homeostatic states (hunger and satiety) influence the cognitive systems regulating impulsive responses, but the direction and specific mechanisms involved in this effect remain elusive. We examined how fasting, and satiety, affect cognitive mechanisms underpinning disinhibition using a novel framework and a gamified test-battery. Thirty-four participants completed the test-battery measuring three cognitive facets of disinhibition: attentional control, information gathering and monitoring of feedback, across two experimental sessions: one after overnight fasting and another after a standardised meal. Homeostatic state was assessed using subjective self-reports and biological markers (i.e., blood-derived liver-expressed antimicrobial protein 2 (LEAP-2), insulin and leptin). We found that participants who experienced greater subjective hunger during the satiety session were more impulsive in the information gathering task; results were not confounded by changes in mood or anxiety. Homeostatic state did not significantly influence disinhibition mechanisms linked to attentional control or feedback monitoring. However, we found a significant interaction between homeostatic state and LEAP-2 on attentional control, with higher LEAP-2 associated with faster reaction times in the fasted condition only. Our findings indicate lingering hunger after eating increases impulsive behaviour via reduced information gathering. These findings identify a novel mechanism that may underpin the tendency to overeat and/or engage in broader impulsive behaviours.
Validity and Reproducibility of a Food Frequency Questionnaire to Assess Macro and Micro-Nutrient Intake among a Convenience Cohort of Healthy Adult Qataris
Authors: Bawadi Hiba , Akasheh Rand T. , Kerkadi Abdelhamid , Haydar Salma , Tayyem Reema , Shi Zumin
Published: June 2021

This study aimed at developing a valid culture-sensitive quantitative food frequency questionnaire (FFQ) for Qatari adults. A convenient sample of healthy Qataris (n = 107) were recruited from family members of Qatar University students. The Diet History Questionnaire II of the US National Cancer Institute was translated to Arabic language, back-translated to English, pilot tested, and then modified accordingly to be used in Qatari setting. Participants were asked to complete the translated version of the FFQ. This FFQ was then validated against three 24 h diet recall (24 hDR) including a weekend day. Participants were asked to complete the FFQ again after one-month period to measure its repeatability. Dietary data were analyzed using the dietary analysis software ESHA. The validity and reliability of FFQ were assessed by comparing the median intake of nutrients and foods and by calculating the Pearson correlation coefficients. The median nutrient intakes assessed by the second FFQ were higher than that reported in the baseline FFQ1 except for fat. The percentage of increase varies between 1.5% and 96%. Results of the second FFQ indicated an overestimation of intake for most nutrients (macro and micro). Macronutrient intakes assessed by the two FFQ and 24 hDR were strongly correlated. The correlation coefficients for micronutrient intakes between FFQ2 and 24hDR were lower than that of the two FFQs except for calcium (r = 0.55) and sodium (r = 0.643). They ranged from (−0.17) for fluorine to (0.643) for sodium. The agreement rates for classifying macronutrient intakes into same or adjacent quartile were between 79.4% and 100% for the two FFQs and between 71% and 100% for the second FFQ and 24hDR. The reported consumption of food groups estimated by FFQ2 was significantly higher than that reported by FFQ1. In conclusion, the developed FFQ was sufficiently valid to assess energy and macronutrients but not micronutrients. The reliability was adequate for most nutrients.
Curcumin and Its Potential Impact on Microbiota
Authors: Jabczyk Marzena , Nowak Justyna , Hudzik Bartosz , Zubelewicz-Szkodzińska Barbara
Published: June 2021

Curcumin is one of the most frequently researched herbal substances; however, it has been reported to have a poor bioavailability and fast metabolism, which has led to doubts about its effectiveness. Curcumin has antioxidant and anti-inflammatory effects, and has demonstrated favorable health effects. Nevertheless, well-reported in vivo pharmacological activities of curcumin are limited by its poor solubility, bioavailability, and pharmacokinetic profile. The bidirectional interactions between curcumin and gut microbiota play key roles in understanding the ambiguity between the bioavailability and biological activity of curcumin, including its wider health impact.
Are We Closer to International Consensus on the Term ‘Food Literacy’? A Systematic Scoping Review of Its Use in the Academic Literature (1998–2019)
Authors: Thompson Courtney , Adams Jean , Vidgen Helen Anna
Published: June 2021

(1) Background: The term ‘food literacy’ has gained momentum globally; however, a lack of clarity around its definition has resulted in inconsistencies in use of the term. Therefore, the objective was to conduct a systematic scoping review to describe the use, reach, application and definitions of the term ‘food literacy’ over time. (2) Methods: A search was conducted using the PRISMA-ScR guidelines in seven research databases without any date limitations up to 31 December 2019, searching simply for use of the term ‘food literacy’. (3) Results: Five hundred and forty-nine studies were included. The term ‘food literacy’ was used once in 243 articles (44%) and mentioned by researchers working in 41 countries. Original research was the most common article type (n = 429, 78%). Food literacy was published across 72 In Cites disciplines, with 456 (83%) articles from the last 5 years. In articles about food literacy (n = 82, 15%), review articles were twice as prevalent compared to the total number of articles (n = 10, 12% vs. n = 32, 6%). Fifty-one different definitions of food literacy were cited. (4) Conclusions: ‘Food literacy’ has been used frequently and broadly across differing article types and disciplines in academic literature internationally. However, agreement on a standardised definition of food literacy endorsed by a peak international agency is needed in order to progress the field.
Adherence to Mediterranean Diet Measured through Medi-Lite Score and Obesity: A Retrospective Study
Authors: Dinu Monica , Pagliai Giuditta , Lotti Sofia , Giangrandi Ilaria , Colombini Barbara , Sofi Francesco
Published: June 2021

We recently developed and validated a questionnaire to measure adherence to the Mediterranean diet, called Medi-Lite. The aim of this study was to assess the accuracy of the Medi-Lite adherence score in relation to obesity status. A total of 208 patients who attended the Clinical Nutrition Unit of Careggi University Hospital, Florence, were included in this retrospective analysis. Of them, 126 (45%) had abdominal obesity (110 F; 16 M). The mean adherence score, calculated through the Medi-Lite questionnaire, was 9.5 ± 2.2, with significantly (p < 0.001) lower values in patients with abdominal obesity (8.9 ± 1.9) than those without abdominal obesity (10 ± 2.2). Logistic regression analysis adjusted for age and sex showed that the Medi-Lite score determined significant protection (−28%) against the risk of abdominal obesity for every one-unit increase in the total score (OR 0.72, 95% CI 0.63–0.82; p < 0.001). Looking for cut-off values that denote increased risk of having abdominal obesity, we observed that patients who scored ≤9 had a significantly increased risk (OR 3.21, 95% CI 1.91–5.39; p < 0.001). Adherence to the Mediterranean diet assessed through the Medi-Lite score was found to be associated with abdominal obesity. In particular, patients who reported a score of ≤9 had a 3.5-fold times higher risk of having abdominal obesity than those who scored >9.
Riboswitches for Controlled Expression of Therapeutic Transgenes Delivered by Adeno-Associated Viral Vectors
Authors: Tickner Zachary J. , Farzan Michael
Published: June 2021

Vectors developed from adeno-associated virus (AAV) are powerful tools for in vivo transgene delivery in both humans and animal models, and several AAV-delivered gene therapies are currently approved for clinical use. However, AAV-mediated gene therapy still faces several challenges, including limited vector packaging capacity and the need for a safe, effective method for controlling transgene expression during and after delivery. Riboswitches, RNA elements which control gene expression in response to ligand binding, are attractive candidates for regulating expression of AAV-delivered transgene therapeutics because of their small genomic footprints and non-immunogenicity compared to protein-based expression control systems. In addition, the ligand-sensing aptamer domains of many riboswitches can be exchanged in a modular fashion to allow regulation by a variety of small molecules, proteins, and oligonucleotides. Riboswitches have been used to regulate AAV-delivered transgene therapeutics in animal models, and recently developed screening and selection methods allow rapid isolation of riboswitches with novel ligands and improved performance in mammalian cells. This review discusses the advantages of riboswitches in the context of AAV-delivered gene therapy, the subsets of riboswitch mechanisms which have been shown to function in human cells and animal models, recent progress in riboswitch isolation and optimization, and several examples of AAV-delivered therapeutic systems which might be improved by riboswitch regulation.
Unexpected Salt/Cocrystal Polymorphism of the Ketoprofen–Lysine System: Discovery of a New Ketoprofen–l-Lysine Salt Polymorph with Different Physicochemical and Pharmacokinetic Properties
Authors: Aramini Andrea , Bianchini Gianluca , Lillini Samuele , Bordignon Simone , Tomassetti Mara , Novelli Rubina , Mattioli Simone , Lvova Larisa , Paolesse Roberto , Chierotti Michele Remo , Allegretti Marcello
Published: June 2021

Ketoprofen–l-lysine salt (KLS) is a widely used nonsteroidal anti-inflammatory drug. Here, we studied deeply the solid-state characteristics of KLS to possibly identify new polymorphic drugs. Conducting a polymorph screening study and combining conventional techniques with solid-state nuclear magnetic resonance, we identified, for the first time, a salt/cocrystal polymorphism of the ketoprofen (KET)–lysine (LYS) system, with the cocrystal, KET–LYS polymorph 1 (P1), being representative of commercial KLS, and the salt, KET–LYS polymorph 2 (P2), being a new polymorphic form of KLS. Interestingly, in vivo pharmacokinetics showed that the salt polymorph has significantly higher absorption and, thus, different pharmacokinetics compared to commercial KLS (cocrystal), laying the basis for the development of faster-release/acting KLS formulations. Moreover, intrinsic dissolution rate (IDR) and electronic tongue analyses showed that the salt has a higher IDR, a more bitter taste, and a different sensorial kinetics compared to the cocrystal, suggesting that different coating/flavoring processes should be envisioned for the new compound. Thus, the new KLS polymorphic form with its different physicochemical and pharmacokinetic characteristics can open the way to the development of a new KET–LYS polymorph drug that can emphasize the properties of commercial KLS for the treatment of acute inflammatory and painful conditions.
Modelling and Simulation of the Drug Release from a Solid Dosage Form in the Human Ascending Colon: The Influence of Different Motility Patterns and Fluid Viscosities
Authors: Schütt Michael , Stamatopoulos Konstantinos , Batchelor Hannah K. , Simmons Mark J. H. , Alexiadis Alessio
Published: June 2021

For colonic drug delivery, the ascending part of the colon is the most favourable site as it offers the most suitable environmental conditions for drug dissolution. Commonly, the performance of a drug formulation is assessed using standardised dissolution apparatus, which does not replicate the hydrodynamics and shear stress evoked by wall motion in the colon. In this work, computer simulations are used to analyse and understand the influence of different biorelevant motility patterns on the disintegration/drug release of a solid dosage form (tablet) under different fluid conditions (viscosities) to mimic the ascending colonic environment. Furthermore, the ability of the motility pattern to distribute the drug in the ascending colon luminal environment is analysed to provide data for a spatiotemporal concentration profile. The motility patterns used are derived from in vivo data representing different motility patterns in the human ascending colon. The applied motility patterns show considerable differences in the drug release rate from the tablet, as well as in the ability to distribute the drug along the colon. The drug dissolution/disintegration process from a solid dosage form is primarily influenced by the hydrodynamic and shear stress it experiences, i.e., a combination of motility pattern and fluid viscosity. Reduced fluid motion leads to a more pronounced influence of diffusion in the tablet dissolution process. The motility pattern that provoked frequent single shear stress peaks seemed to be more effective in achieving a higher drug release rate. The ability to simulate drug release profiles under biorelevant colonic environmental conditions provides valuable feedback to better understand the drug formulation and how this can be optimised to ensure that the drug is present in the desired concentration within the ascending colon.