bluuebunny/crossref_metadata_2025 huggingface dataset:
The serological IgG and neutralizing antibody of SARS-CoV-2 omicron variant reinfection in Jiangsu Province, China
Authors: Chu Jinjin , Dai Qigang , Dong Chen , Kong Xiaoxiao , Tian Hua , Li Chuchu , Peng Jiefu , Xu Ke , Ju Hao , Bao Changjun , Hu Jianli , Zhu Liguo
Published: May 2024

Background

It is important to figure out the immunity of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) reinfection to understand the response of humans to viruses. A serological survey for previously infected populations in Jiangsu Province was conducted to compare the antibody level of SARS-CoV-2 in reinfection by Omicron or not.

Methods

Reinfection with SARS-CoV-2 was defined as an individual being infected again after 90 days of the initial infection. Telephone surveys and face-to-face interviews were implemented to collect information. Experimental and control serum samples were collected from age-sex-matched reinfected and non-reinfected cases, respectively. IgG anti-S and neutralizing antibodies (Nab) concentrations were detected by the Magnetism Particulate Immunochemistry Luminescence Method (MCLIA). Antibody titers were log(2)-transformed and analyzed by a two-tailed Mann–Whitney U test. Subgroup analysis was conducted to explore the relationship between the strain type of primary infection, SARS-Cov-2 vaccination status, and antibody levels. Multivariate linear regression models were used to identify associations between reinfection with IgG and Nab levels.

Results

Six hundred thirty-one individuals were enrolled in this study, including 327 reinfected cases and 304 non-reinfected cases. The reinfection group had higher IgG (5.65 AU/mL vs. 5.22 AU/mL) and Nab (8.02 AU/mL vs. 7.25 AU/mL) levels compared to the non-reinfection group (p < 0.001). Particularly, individuals who had received SARS-CoV-2 vaccination or were initially infected with the Wild type and Delta variant showed a significant increase in antibody levels after reinfection. After adjusting demographic variables, vaccination status and the type of primary infection together, IgG and Nab levels in the reinfected group increased by log(2)-transformed 0.71 and 0.64 units, respectively (p < 0.001). This revealed that reinfection is an important factor that affects IgG and Nab levels in the population.

Conclusion

Reinfection with Omicron in individuals previously infected with SARS-CoV-2 enhances IgG and Nab immune responses.
Associations between special diet and incidence risk of osteoporosis: a Mendelian randomization study
Authors: Zhou Changwen , Yang Lixue , Liu Ce , Ma Hongzhong , Yang Feng , Chen Liheng
Published: May 2024

Introduction

Osteoporosis is a prevalent challenge in clinical orthopedics, affecting a significant percentage of individuals aged 50 and above. The goal of this study was to comprehensively understand the relationships between a specialized dietary regimen and the risk of developing osteoporosis.

Methods

This study employed extensive genome-wide association study (GWAS) summary statistics derived from the UK Biobank. It encompassed 8 kinds of special diets and 7 datasets pertaining to osteoporosis and associated symptoms. The principal analytical approach employed was the inverse-variance weighted method. Additionally, sensitivity analysis was employed to elucidate the diverse multiplicity patterns observed in the final model.

Results

Our results showed that there is significant evidence that a gluten-free diet is associated with osteoporosis [odds ratio (OR): 1.080, 95% confidence interval (CI): 1.048–1.112, p = 4.23E-07)]. Furthermore, there exists a suggestive link between the three distinct dietary approaches and osteoporosis [(OR: 0.949, 95%CI: 0.929–0.970, p = 3.00E-06) for comprehensive consumption; (OR: 1.053, 95%CI: 1.018–1.089, p = 2.23E-03) for abstaining from wheat consumption; (OR: 1.036, 95%CI: 1.005–1.068, p = 1.97E-02) for abstaining from sugar consumption]. No additional correlation between the special dietary regimens and osteoporosis has been observed.

Conclusion

Our research has uncovered a notable correlation between a gluten-free diet and the occurrence of osteoporosis. Furthermore, it exerts a promoting influence on the onset of osteoporosis, which stands in direct contradiction to the therapeutic principles for Celiac Disease’s complications. As such, a novel association among these three elements is postulated.
Low back pain prevention behaviors and beliefs among the Polish population in a cross-sectional survey
Authors: Kuśmierek Paulina , Mikołajczyk Mateusz , Złotkowska Dagmara , Łowczak Anna , Mikołajczyk Anita
Published: May 2024

Background

Low back pain (LBP) is one of the most common problems of public health and creates a burden globally. The aim was to assess the Polish population’s back pain prevention behaviors and beliefs and to examine how these health behaviors and beliefs vary across sociodemographic factors and physical activity.

Methods

A cross-sectional survey was carried out among 208 randomly selected patients of the public general practitioner clinic. The differences in LBP-related beliefs and attitudes were determined due to participants’ status of requiring or non-requiring LBP treatment.

Results

More than half of the respondents did not engage in behaviors that protect against back pain. Individuals with higher education levels and those who exercised at least once a week were significantly more likely to adopt behaviors to protect their backs. Less than half of the participants reported having a workplace that was adequately prepared to protect against back pain, and only 35.1% of the participants reported receiving instruction while taking up work on how to avoid back pain while working. According to respondents’ opinions, preventive actions are necessary to protect against back pain. Inappropriate exercises and stress can be contributors to back pain, with these opinions reported more often by women and participants with higher education levels. Participants who received treatment for LBP showed a significantly higher expression of behaviors to protect against back pain compared to participants who did not require treatment. However, there were no significant differences in participants’ beliefs about back pain prevention between the group requiring LBP treatment and the group not requiring LBP treatment.

Conclusion

The study provides valuable insights into the association between LBP treatment, back pain prevention behaviors, and beliefs, suggesting potential avenues for future research and intervention development. By addressing workplace ergonomics and promoting a culture of back health, it may be possible to reduce the burden of LBP in Poland.
Urban green spaces and resident health: an empirical analysis from data across 30 provinces in China
Authors: Bi Yan , Wang Ya , Yang Ding , Mao Jialin , Wei Qifeng
Published: May 2024

Background

This study aims to explore the correlation between urban green space coverage and resident health, and to analyze its underlying mechanisms.

Methods

Using panel data from 30 provinces in China from 2006 to 2022, which mainly includes urban green space coverage, general health of the population, air quality, and social connectivity. This research constructed a fixed effects model to perform baseline regression analysis. A series of robustness tests, including variable substitution, controlling for geographical differences, regional robustness tests, and shortening the time span of the study, further verified the robustness of the results. Additionally, mechanism tests were conducted to examine the positive impacts of urban green spaces on resident health by improving air quality and enhancing social connectivity.

Results

The findings indicate a significant positive correlation between urban green space coverage and resident health levels. That is, the greater the area covered with urban green space, the healthier the residents of the area will be. Robustness tests support the reliability of this finding, while mechanism analysis reveals that urban green spaces have a positive impact on the health of the population by improving air quality and increasing social connectivity.

Discussion

This study underscores the importance of urban green space planning in improving resident health and quality of life, providing urban planners with scientific evidence to optimize urban green systems for broader health objectives.
Quantum pathways for charged track finding in high-energy collisions
Authors: Brown Christopher , Spannowsky Michael , Tapper Alexander , Williams Simon , Xiotidis Ioannis
Published: May 2024

In high-energy particle collisions, charged track finding is a complex yet crucial endeavor. We propose a quantum algorithm, specifically quantum template matching, to enhance the accuracy and efficiency of track finding. Abstracting the Quantum Amplitude Amplification routine by introducing a data register, and utilizing a novel oracle construction, allows data to be parsed to the circuit and matched with a hit-pattern template, without prior knowledge of the input data. Furthermore, we address the challenges posed by missing hit data, demonstrating the ability of the quantum template matching algorithm to successfully identify charged-particle tracks from hit patterns with missing hits. Our findings therefore propose quantum methodologies tailored for real-world applications and underline the potential of quantum computing in collider physics.
Accelerating human–computer interaction through convergent conditions for LLM explanation
Authors: Raikov Aleksandr , Giretti Alberto , Pirani Massimiliano , Spalazzi Luca , Guo Meng
Published: May 2024

The article addresses the accelerating human–machine interaction using the large language model (LLM). It goes beyond the traditional logical paradigms of explainable artificial intelligence (XAI) by considering poor-formalizable cognitive semantical interpretations of LLM. XAI is immersed in a hybrid space, where humans and machines have crucial distinctions during the digitisation of the interaction process. The author’s convergent methodology ensures the conditions for making XAI purposeful and sustainable. This methodology is based on the inverse problem-solving method, cognitive modeling, genetic algorithm, neural network, causal loop dynamics, and eigenform realization. It has been shown that decision-makers need to create unique structural conditions for information processes, using LLM to accelerate the convergence of collective problem solving. The implementations have been carried out during the collective strategic planning in situational centers. The study is helpful for the advancement of explainable LLM in many branches of economy, science and technology.
Bringing rigor in contextual objectivity: lessons from applying feminist lens in scoping the evidence on girlhood studies in Indonesia
Authors: Kusumaningrum Santi , Tieken Shaila , Adhi Andrea Andjaringtyas , Nisa Siti Ainun , Sari Widi Laras , Beta Annisa R.
Published: May 2024

This perspective paper contemplates the nuances of engaging with literature ethically in conducting a scoping review based on the researchers' project on girlhood studies in Indonesia. We assert that the ethical perspective extends beyond conventional primary data collection from human participants, further emphasizing the essence of a feminist methodology in this scholarly investigation. We discuss the interplay between the role of rigor and the dynamics of power relations in research, shedding light on reconciling between the pursuit of facts and acknowledgment of biases in knowledge production. This reflection offers insights into the methodological process and the researcher's role, contributing to the broader discourse on how research can effectively address issues of gender equity and social inclusion. Through this paper, we underscore the necessity of an intentional approach in unifying the domains of science and advocacy because only then can we truly catalyze transformative change. In doing so, we seek to foster a more comprehensive, objective, and empathetic understanding of the researched: in this case, the experiences of girls and young women –and, by extension, marginalized individuals in Indonesia and beyond.
Haptic based fundamentals of laparoscopic surgery simulation for training with objective assessments
Authors: Abinaya P. , Manivannan M.
Published: May 2024

Force is crucial for learning psychomotor skills in laparoscopic tissue manipulation. Fundamental laparoscopic surgery (FLS), on the other hand, only measures time and position accuracy. FLS is a commonly used training program for basic laparoscopic training through part tasks. The FLS is employed in most of the laparoscopic training systems, including box trainers and virtual reality (VR) simulators. However, many laparoscopic VR simulators lack force feedback and measure tissue damage solely through visual feedback based on virtual collisions. Few VR simulators that provide force feedback have subjective force metrics. To provide an objective force assessment for haptic skills training in the VR simulators, we extend the FLS part tasks to haptic-based FLS (HFLS), focusing on controlled force exertion. We interface the simulated HFLS part tasks with a customized bi-manual haptic simulator that offers five degrees of freedom (DOF) for force feedback. The proposed tasks are evaluated through face and content validity among laparoscopic surgeons of varying experience levels. The results show that trainees perform better in HFLS tasks. The average Likert score observed for face and content validity is greater than 4.6 ± 0.3 and 4 ± 0.5 for all the part tasks, which indicates the acceptance of the simulator among subjects for its appearance and functionality. Face and content validations show the need to improve haptic realism, which is also observed in existing simulators. To enhance the accuracy of force rendering, we incorporated a laparoscopic tool force model into the simulation. We study the effectiveness of the model through a psychophysical study that measures just noticeable difference (JND) for the laparoscopic gripping task. The study reveals an insignificant decrease in gripping-force JND. A simple linear model could be sufficient for gripper force feedback, and a non-linear LapTool force model does not affect the force perception for the force range of 0.5–2.5 N. Further study is required to understand the usability of the force model in laparoscopic training at a higher force range. Additionally, the construct validity of HFLS will confirm the applicability of the developed simulator to train surgeons with different levels of experience.
Local super apps in the 15-minute city: a new model for sustainable smart cities?
Authors: Hasselwander Marc , Weiss Daniel , Werland Stefan
Published: May 2024

In this perspective paper, we propose to integrate the concepts of Mobility-as-a-Feature (MaaF, an extension of MaaS) and the 15-minute city (15mC). The 15mC concept maintains that daily necessities and services, such as shopping, healthcare, and leisure should be accessible without private cars within 15 minute. In line with MaaF, these services could be integrated with a variety of mobility options into a single app. This novel approach is poised to offer a seamless customer experience, better resource utilization, enhanced urban mobility, improved and more inclusive access to services, and greater community connectivity. We call them local super apps: a new model to drive equitable and sustainable urban transitions. We substantiate this preliminary idea with evidence from literature, practical applications, and a user survey (N = 1,019), while also discussing future research avenues to further develop the concept of local super apps.
Emotion dysregulation in insomnia disorder: the possible role of psychiatric comorbidity
Authors: Jansson-Fröjmark Markus , Hossain Samiul
Published: May 2024

Aim

The purpose of this study was to investigate the link between emotion dysregulation and insomnia disorder as well as the possible role of psychiatric comorbidity on the association. More specifically, the aim was to examine whether the elevations in emotion dysregulation in insomnia are dependent on co-occurring psychiatric comorbidity, in this study defined as anxiety disorders and/or major depression.

Methods

Four diagnostically differing groups with 25 participants in each were recruited: normal sleep, normal sleep with psychiatric comorbidity, insomnia disorder, and insomnia disorder with psychiatric comorbidity. The 100 study participants completed self-report scales and items assessing socio-demographic parameters, symptoms of insomnia, anxiety, and depression, generic emotion regulation, and insomnia-specific emotion regulation.

Results

Concerning generic emotion regulation, the results showed that psychiatric comorbidity, but not insomnia, was associated with elevations in generic emotion dysregulation. Psychiatric comorbidity was distinctly related to elevations in non-acceptance, goals, and impulse domains (d = 1.09–1.22). Regarding insomnia-specific emotion regulation, the findings demonstrated that insomnia, with or without psychiatric comorbidity, was related to heightened use of insomnia-associated emotion dysregulation strategies. Insomnia was uniquely associated with elevated unhelpful beliefs about sleep and safety behaviors (d = 1.00–1.04).

Conclusion

The current findings support the notion that insomnia is associated with specific, but not generic, emotion dysregulation strategies. These results have relevance for the conceptualization of the role of emotion dysregulation in insomnia and the clinical management of insomnia.
The impact of improper waste disposal on human health and the environment: a case of Umgungundlovu District in KwaZulu Natal Province, South Africa
Authors: Raphela Tlou , Manqele Nelisiwe , Erasmus Mariana
Published: May 2024

Introduction

Waste generation has increased drastically around the world in recent decades, with less than 20% of waste recycled each year, and one-third of all food produced wasted. With Sustainable Development Goal 12 advocating for changing how we consume, produce, and dispose of items, the cruciality of driving a more sustainable future lies in how we dispose of our waste.

Methodology

This study assessed the impacts of improper waste disposal on human health and the environment in the KwaZulu Natal Province of South Africa. The study applied a mixed-method pragmatic research approach, using Statistical Package for Social Scientists, and applied a series of Chi-Squared tests of independence, regression, and descriptive statistics to analyse data. This study has shed light on the complex dynamics surrounding the respondents’ awareness and perception of risks associated with improper waste disposal.

Results

While a fair level of knowledge exists concerning the general risks, there are notable gaps in understanding specific aspects of human health risks related to improper waste disposal. Furthermore, the study findings highlight a critical disparity in awareness regarding specific human health risks associated with improper waste disposal.

Discussion

This study has shed light on the complex dynamics surrounding the respondents’ awareness and perception of risks associated with improper waste disposal. This underscores the importance of enhancing public education and outreach programs to ensure a comprehensive understanding of the potential dangers to human health. The study municipality should explore sustainable waste management practices to mitigate the economic burden posed by increased waste generation.
Volunteer programs, empowerment, and life satisfaction in Jordan: mapping local knowledge and systems change to inform public policy and science diplomacy
Authors: Panter-Brick Catherine , Qtaishat Lina , Eggerman Jannik Joseph , Thomas Honey , Kumar Praveen , Dajani Rana
Published: May 2024

Introduction

Volunteering in the community is thought to provide unique benefits to people who experience limited engagement in society. In the global South, volunteer programs are often framed as empowering women and benefiting the poor, without empirical evidence or systematic investigation of what this means from a local perspective. For this reason, it is critical to represent stakeholder knowledge, understand how change happens systemically, and reduce cultural bias in scientific inquiry and public policy. As such, efforts to respect diverse narratives and problem-solving approaches are key to science diplomacy – they help us understand cultural relevance, program efficacy, and for whom a program is considered transformative.

Methods and results

This study shows how Syrian refugee and Jordanian women, living in resource-poor families, articulated (i) concepts of empowerment and life satisfaction and (ii) the benefits of engaging in community-based volunteering programs. Through engaging in a participatory methodology known as Fuzzy Cognitive Mapping, women generated visual representations of these constructs and cause-and-effect reasoning. They identified several dimensions of empowerment (e.g., cultural, financial, and psychological empowerment) and several meanings of life satisfaction (e.g. adaptation, acceptance, and contentment). They also mapped connections between variables, identifying those that might catalyze change. We were specifically interested in evaluating understandings of We Love Reading, a program that trains volunteers to become changemakers in their local community. In simulations, we modelled how employment, education, money, and volunteering would drive system change, with notable results on cultural empowerment.

Discussion

Through visual maps and scenarios of change, the study demonstrates a participatory approach to localizing knowledge and evaluating programs. This is key to improving scientific enquiry and public policy.
How do nuclear isomers influence the gamma-ray bursts in binary neutron star mergers?
Authors: Hamilton Maria C. Babiuc , Powell Joseph I.
Published: May 2024

Neutron star mergers are astrophysical “gold mines,” synthesizing over half of the elements heavier than iron through rapid neutron capture nucleosynthesis. The observation of the binary neutron star merger GW170817, detected both in gravitational waves and electromagnetic radiation, marked a breakthrough. One electromagnetic component of this event, the gamma ray burst GRB 170817A, has an unresolved aspect: the characteristics of its prompt gamma-ray emission spectrum. In this work, we investigate that gamma-ray spectra in such GRBs may be influenced by de-excitations from isomeric transitions. Our study begins with a review of current knowledge on GRB structure and of r-process nucleosynthesis in neutron star collisions, focusing on the role of nuclear isomers in these settings. We then test our hypothesis by developing criteria to select representative isomers, based on known solar element abundances, for modeling GRB spectral characteristics. We integrate these criteria into an interactive web page, facilitating the construction and analysis of relevant gamma-ray spectra from isomeric transitions. Our analysis reveals that three isomers—90Zr, 207Pb, and 89Y—stand out for their potential to impact the prompt GRB spectrum due to their specific properties. This information allows us to incorporate nuclear isomer data into astrophysical simulations and calculate isomeric abundances generated by astrophysical r-processes in neutron star mergers and their imprint on the detected signal.
Magnetopause MHD surface wave theory: progress & challenges
Authors: Archer Martin O. , Pilipenko Vyacheslav A. , Li Bo , Sorathia Kareem , Nakariakov Valery M. , Elsden Tom , Nykyri Katariina
Published: May 2024

Sharp boundaries are a key feature of space plasma environments universally, with their wave-like motion (driven by pressure variations or flow shears) playing a key role in mass, momentum, and energy transfer. This review summarises magnetohydrodynamic surface wave theory with particular reference to Earth’s magnetopause, due to its mediation of the solar-terrestrial interaction. Basic analytic theory of propagating and standing surface waves within simple models are presented, highlighting many of the typically-used assumptions. We raise several conceptual challenges to understanding the nature of surface waves within a complex environment such as a magnetosphere, including the effects of magnetic topology and curvilinear geometry, plasma inhomogeneity, finite boundary width, the presence of multiple boundaries, turbulent driving, and wave nonlinearity. Approaches to gain physical insight into these challenges are suggested. We also discuss how global simulations have proven a fruitful tool in studying surface waves in more representative environments than analytic theory allows. Finally, we highlight strong interdisciplinary links with solar physics which might help the magnetospheric community. Ultimately several upcoming missions provide motivation for advancing magnetopause surface wave theory towards understanding their global role in filtering, accumulating, and guiding turbulent solar wind driving.
More sustainable use of aquaculture cleaner fish: collagen-rich protein hydrolysates from lumpfish (Cyclopterus lumpus) – effects of biomass, pretreatment, and enzyme choice
Authors: Lian Kjersti , Maribu Ingrid , Rode Tone Mari , Jenssen Marte , Vang Birthe , Solstad Runar Gjerp
Published: May 2024

Farmed lumpfish (Cyclopterus lumpus) are used as cleaner fish in Norwegian aquaculture. However, once the fish reaches sexual maturity, it becomes less effective in combating lice and is often wasted or turned into silage. This raises ethical, economic, and sustainability concerns regarding whether the use is sustainable without increasing its standard of living and further use of the fish to higher value products. To improve the utilization of lumpfish, a study was conducted to investigate the differences in extraction efficiency by comparing product yield, protein content, and peptide size distribution after enzymatic hydrolysis of lumpfish using several commercial proteases (Corolase 8,000, Corolase 7,089, Endocut 01 L, Flavourzyme, and Food Pro PNL), and different pretreatment methods (pulsed electric field and high-pressure processing). The choice of enzyme did not affect the amino acid contents of the different hydrolysates. Furthermore, varying enzyme concentrations had a minor impact on the final product’s protein content. As anticipated, increased enzyme concentrations decreased the hydrolysates’ average molecular weight. The study found that biomass treated either chemically (NaOH and butanol) or mechanically with a pulsed electric field before enzymatic hydrolysis resulted in higher protein content and relatively higher amounts of collagen peptides. Initial bioactivity testing indicated that the hydrolysates had no toxic effects on hepatocellular carcinoma and non-malignant lung fibroblast cells. Previous studies have shown that farmed lumpfish contains all the essential amino acids and has high levels of EPA, DHA, B12, and D3 vitamins. The contents of environmental pollutants and heavy metals were also below the EU maximum levels. This study’s knowledge and results open for the potential use of lumpfish peptides and gelatin for, e.g., dietary supplements, feed, and biodegradable packaging.
Morphology, morphometry, and phylogeny of the protozoan parasite, Eimeria labbeana-like (Apicomplexa, Eimeriidae), infecting Columba livia domestica
Authors: Albasyouni Shurug , Abdel-Gaber Rewaida , Al Quraishy Saleh , Al-Shaebi Esam M. , Mohammed Osama B.
Published: May 2024

Introduction

Eimeria spp. are intracellular protozoan parasites of the phylum Apicomplexa causing economic losses to various wild and domestic animals. An eimerian species infecting Columba livia domestica was identified in this study.

Methods

A total of 15 faecal samples were examined by floatation technique, a prevalence rate of 60% was reported. Eimerian oocysts were sporulated in 2.5% potassium dichromate solution then identified using morphological and molecular (DNA amplification of the 18S rRNA and ITS-1 genes) diagnostic techniques.

Results

Sporulated oocysts were identified as Eimeria labbeana-like, after morphometry with typical bi-layered wall with spherical to subspherical oocysts morphology. A polar granule is present, but no micropyle or oocyst residuum. Sporocysts are elongated ovoidal with stieda body. Sporocyst residuum with many granules and sporozoites with refractile bodies and nucleus. Both 18S rRNA and ITS-1 sequences have been deposited in GenBank database. DNA sequences from the partial 18S rRNA generated from the oocysts were found to be related to eimerian and isosporan parasites found in domestic pigeons. For the first time, ITS-1 sequences for E. labbeana-like were provided.

Conclusion

The necessity of using molecular techniques to describe pigeon intestinal coccidian parasites in conjunction with traditional morphology-based tools was emphasized in this work in order to understand the biology of such parasites.
Molecular characterization of Cryptosporidium in wild rodents from the Inner Mongolian Autonomous Region and Liaoning Province, China: assessing host specificity and the potential for zoonotic transmission
Authors: Liu Li , Xu Qunfang , Jiang Aiying , Zeng Fansheng , Zhao Wei , Tan Feng
Published: May 2024

Introduction

Wild rodents are key hosts for Cryptosporidium transmission, yet there is a dearth of information regarding their infection status in the Inner Mongolian Autonomous Region and Liaoning Province of China. Therefore, the present study was conducted to determine the prevalence and genetic characteristics of Cryptosporidium among wild rodents residing in these two provinces.

Methods

A total of 486 rodents were captured, and fresh feces were collected from each rodent’s intestine for DNA extraction. Species identification of rodents was performed through PCR amplification of the vertebrate cytochrome b (cytb) gene. To detect the presence of Cryptosporidium in all fecal samples, PCR analysis and sequencing of the partial small subunit of the ribosomal RNA (rRNA) gene were performed.

Results

Four species of rodents were identified: Rattus norvegicus, Mus musculus, Apodemus agrarius, and Cricetulus barabensis. Positive results for Cryptosporidium were obtained for 9.2% (18/195), 6.6% (7/106), 5.6% (5/89), and 6.3% (6/96) of these rodents, respectively, with an average infection rate of 7.4% (36/486). The identification revealed the presence of five Cryptosporidium species, C. ubiquitum (n = 8), C. occultus (n = 5), C. muris (n = 2), C. viatorum (n = 1), and C. ratti (n = 1), along with two Cryptosporidium genotypes: Rat genotype III (n = 10) and Rat genotype IV (n = 9).

Discussion

Based on the molecular evidence presented, the wild rodents investigated were concurrently infected with zoonotic (C. muris, C. occultus, C. ubiquitum and C. viatorum) as well as rodent-adapted (C. ratti and Rat genotype III and IV) species/genotypes, actively participating in the transmission of cryptosporidiosis.
Genome-wide association analysis of fleece traits in Northwest Xizang white cashmere goat
Authors: Lu Xiaotian , Suo Langda , Yan Xiaochun , Li Wenze , Su Yixin , Zhou Bohan , Liu Can , Yang Lepu , Wang Jiayin , Ji De , Cuomu Renqing , Cuoji Awang , Gui Ba , Wang Zhiying , Jiang Wei , Wu Yujiang , Su Rui
Published: May 2024

Northwest Xizang White Cashmere Goat (NXWCG) is the first new breed of cashmere goat in the Xizang Autonomous Region. It has significant characteristics of extremely high fineness, gloss, and softness. Genome-wide association analysis is an effective biological method used to measure the consistency and correlation of genotype changes between two molecular markers in the genome. In addition, it can screen out the key genes affecting the complex traits of biological individuals. The aim of this study was to analyze the genetic mechanism of cashmere trait variation in NXWCG and to discover SNP locus and key genes closely related to traits such as superfine cashmere. Additionally, the key genes near the obtained significant SNPs were analyzed by gene function annotation and biological function mining. In this study, the phenotype data of the four traits (cashmere length, fiber length, cashmere diameter, and cashmere production) were collected. GGP_Goat_70K SNP chip was used for genotyping the ear tissue DNA of the experimental group. Subsequently, the association of phenotype data and genotype data was performed using Gemma-0.98.1 software. A linear mixed model was used for the association study. The results showed that four fleece traits were associated with 18 significant SNPs at the genome level and 232 SNPs at the chromosome level, through gene annotated from Capra hircus genome using assembly ARS1. A total of 107 candidate genes related to fleece traits were obtained. Combined with Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis, we can find that CLNS1A, CCSER1, RPS6KC1, PRLR, KCNRG, KCNK9, and CLYBL can be used as important candidate genes for fleece traits of NXWCG. We used Sanger sequencing and suitability chi-square test to further verify the significant loci and candidate genes screened by GWAS, and the results show that the base mutations loci on the five candidate genes, CCSER1 (snp12579, 34,449,796, A → G), RPS6KC1 (snp41503, 69,173,527, A → G), KCNRG (snp41082, 67,134,820, G → A), KCNK9 (14:78472665, 78,472,665, G → A), and CLYBL (12: 9705753, 9,705,753, C → T), significantly affect the fleece traits of NXWCG. The results provide a valuable basis for future research and contribute to a better understanding of the genetic structure variation of the goat.
Prebiotic fiber blend supports growth and development and favorable digestive health in puppies
Authors: McGrath Allison P. , Motsinger Laura A. , Brejda John , Hancock Leslie
Published: May 2024

Introduction

A healthy gastrointestinal (GI) microbiome has been shown to be essential for proper nutrient absorption and metabolism, maintenance of intestinal epithelial integrity and osmolarity, gut immunomodulation, and overall health. One of the most effective ways to promote a healthy GI microbiome is through dietary interventions, such as the addition of prebiotics. Prebiotics are substrates that are selectively utilized by the host GI microbiome through fermentation to confer a health benefit. However, research on prebiotics in companion animals is limited, especially in growing animals. Thus, this study was conducted to assess the effects of a novel prebiotic fiber blend on key parameters related to intestinal health and growth in puppies.

Methods

Twenty-two puppies at least 4 months of age but not older than 10 months were fed a commercially available dry food during a prefeed period, and then fed a similarly formulated test food with the addition of the prebiotic fiber blend for a minimum of 90 days. Serum and fecal samples were collected at the end of the prefeed period and throughout the test period.

Results

Puppies fed the test food grew as expected for puppies of this age. Complete blood count and serum chemistry analyses were clinically normal for all animals. Fecal score increased linearly, fecal moisture decreased linearly, and pH exhibited a cubic trend throughout the study duration. There was a linear increase in short-chain fatty acids throughout the study, which is associated with favorable digestive and overall health. The inflammatory cytokine interleukin-7 decreased linearly and interleukin-18 trended towards linear decrease.

Conclusion

This study showed that puppies continued to grow and develop normally, and experienced serum and stool characteristics indicative of improved GI health when fed a growth food fortified with a novel prebiotic fiber blend. Furthermore, these results contribute to the overall understanding of the effects of prebiotics on the GI health of growing companion animals.
Instrument to Study Plume Surface Interactions (PSI) on the Lunar Surface: Science Motivation, Requirements, Instrument Overview, and Test Plans
Authors: Bueno Ariana , Krasowski Michael J. , Prokop Norman , Greer Lawrence C. , Adams Christina M. , Rennó Nilton O.
Published: May 2024

Safe landings are imperative to accomplish NASA’s Artemis goal to enable human exploration on the Moon, including sample collection missions. However, a process known as plume surface interaction (PSI) presents a significant hazard to lunar landings. PSI occurs when the engine exhaust of a lander interacts with the surface ejecting large amounts of regolith particles at high velocities that can interfere with the landing, disturb the surface, and damage hardware. To better understand PSI, the particle impact event (PIE) sensor is being developed to measure the kinetic energy and the flux of ejecta during landings, to quantify the potential damage, and to quantify the ejecta displaced. Multiple parameters were estimated to define the PIE instrument requirements. These estimates demonstrate that ejecta can travel at velocities of up to 800 m/s and impact the surrounding area with energies of up to 400 µJ. A significant amount of ejecta can be deposited several 10 s of meters away from the landing site, modifying the surface and causing dust-related challenges. The PIE sensor will be launched for the first time in an upcoming lunar lander. Then, PIE measurements will be used to improve PSI prediction capabilities and develop mitigation strategies to ensure safe landings.
Modeling and Analysis of the Flap Actuation System Considering the Nonlinear Factors of EMA, Joint Clearance and Flexibility
Authors: Wan Qi , Song Chunyu , Zhou Yong , Tong Ruiting , Ma Shangjun , Liu Geng
Published: May 2024

The performance of the flap actuation system directly affects the control effect and the flight quality of an aircraft. The electromechanical actuator (EMA) and the linkage mechanism are important components of the system. In order to achieve the goals of good transmission accuracy and dynamic response, the influence of nonlinear properties in the transmission chain including the EMA and linkage mechanism should be considered. A co-simulation model at the system-level of the flap actuation system was developed, which takes nonlinear factors of the EMA, the impact dynamics of the linkage mechanism with joint clearance and the rigid–flexible coupling characteristics into account. Moreover, the experiments with different command frequencies and loads were performed. The simulation and experimental results were compared to verify the effectiveness of the co-simulation model. Finally, the effects of nonlinear properties including the contact stiffness and clearance of a planetary roller screw mechanism, EMA anchorage stiffness, number of clearance joints, flexibility and load are discussed. This work can contribute to analyzing the performance of an electromechanical multibody system with nonlinear characteristics, which has crucial academic meaning and engineering application values for the development of systems with high speed, good reliability and long life.
Exploring the Psychological Well-Being of Flight Cadets through a Comprehensive Survey Analysis of Self-Awareness and Self-Acceptance
Authors: Miao Dan , Cao Xiaodong , Zhao Bingxu , Shi Yuan , Shi Yunze
Published: May 2024

A robust level of self-awareness and self-acceptance is crucial for flight cadets. In this study, a total of 106 flight cadets from various grades and flight training sites were assessed using the self-awareness and self-acceptance scale. The scales were optimized through item analysis, reliability, and validity assessments. The finalized scales demonstrated an acceptable level of reliability and validity. Upon analyzing the collected data, it was observed that the overall self-awareness and -acceptance levels among the evaluated pilot students fell within the normal range. However, identifying positive symptoms directly proved challenging. The tested flight cadets exhibited moderate symptoms across each factor, with instances of severe symptoms in academic self-awareness. Notably, flight cadets trained abroad exhibited a lower level of self-awareness and -acceptance compared to those trained in China. But this phenomenon was not reflected in grade difference. Regression analysis revealed that physical and emotional self-awareness dimensions accounted for 62% of the variations in the psychological dimension, while passive self-acceptance explained 72% of the changes in active self-acceptance. Finally, in view of the issues found in the research, corresponding management measures and recommendations are presented to enhance the self-awareness and -acceptance levels of flight cadets.
Orbital Pursuit–Evasion–Defense Linear-Quadratic Differential Game
Authors: Li Zhen-Yu
Published: May 2024

To find superior guidance strategies for preventing possible interception threats from space debris, out-of-control satellites, etc., this paper investigates an orbital pursuit–evasion–defense game problem with three players called the pursuer, the evader, and the defender, respectively. In this game, the pursuer aims to intercept the evader, while the evader tries to escape the pursuer. A defender accompanying the evader can protect the evader by actively intercepting the pursuer. For such a game, a linear-quadratic duration-adaptive (LQDA) strategy is first proposed as a basic strategy for the three players. Later, an advanced pursuit strategy is designed for the pursuer to evade the defender when they are chasing the evader. Meanwhile, a cooperative evasion–defense strategy is proposed for the evader and the defender to build their cooperation. Simulations determined that the proposed LQDA strategy has higher interception accuracy than the classic LQ strategy. Meanwhile, the proposed two-sided pursuit strategy can improve the interception performance of the pursuer against a non-cooperative defender. But if the evader and defender employ the proposed cooperation strategy, the pursuer’s interception will be much more difficult.
MEEMD-Based Ground Vibration Component Extraction and Response Analysis of Long Period Ground Vibration on High-Pier Large-Span Bridges
Authors: Li Chunyu , Jia Jingwei , Jia Jie
Published: May 2024

Comparing the empirical modal decomposition (EMD) method and the modified ensemble empirical modal decomposition (MEEMD) method, a response analysis of the long-period components, with the separation and reconstruction of the two methods, and the original long-period ground vibration on high-pier bridges is carried out to verify the validity of the extracted long-period components. In addition, the response characteristics of the components extracted based on the MEEMD and the original ground vibration are studied on the bridges at different points. The results show that, through the correlation of the displacement response of the bridge piers, the long-period component of the long-period ground shaking can well reflect the response characteristics of the long-period ground shaking on the long-period structure, and the MEEMD provides better results than the EMD extraction method. The long-period structure resonates during the long-period component of the long-period ground shaking, the short-period structure resonates during the short-period component, and the structural seismic response is similar to the self-resonance of the structure. The seismic response of the structures is closely related to the self-oscillation period and the spectral parameters of the ground shaking.
Evaluation of Salivary Biomarkers and Spirometry for Diagnosing COPD in Non-Smokers and Smokers of Polish Origin
Authors: Rudzinska-Radecka Magdalena , Bańcerowski Bartłomiej , Marczyński Robert , Mukherjee Debjita , Sikora Tomasz , Morawska Karolina , Mielczarek Agnieszka , Moździerski Marcin , Hajduk Bogdan , Kotowicz Beata
Published: May 2024

Chronic obstructive pulmonary disease (COPD) is a prevalent respiratory condition with global implications. Accurate and timely diagnosis is critical; however, traditional diagnostic methods (based on spirometry) show limitations, prompting the search for predictive biomarkers and modern diagnostic techniques. This study explored the validation of COPD-related biomarkers (C-reactive protein, procalcitonin, neutrophil elastase, and alpha-1 antitrypsin) in saliva. A diverse cohort, including healthy non-smokers, healthy smokers, and COPD patients of Polish origin, underwent spirometry and marker analysis. The data correlated with clinical factors, revealing noteworthy relations. Firstly, salivary biomarker levels were compared with serum concentrations, demonstrating notable positive or negative correlations, depending on the factor. Further analysis within healthy individuals revealed associations between biomarker levels, spirometry, and clinical characteristics such as age, sex, and BMI. Next, COPD patients exhibited an enhanced concentration of biomarkers compared to healthy groups. Finally, the study introduced a breathing assessment survey, unveiling significant associations between self-perceived breathing and spirometric and tested parameters. Outcomes emphasized the relevance of subjective experiences in COPD research. In conclusion, this research underscored the potential of salivary biomarkers as diagnostic tools for COPD, offering a non-invasive and accessible alternative to traditional methods. The findings paved the way for improved modern diagnostic approaches.
Hyperoxia-Induced miR-195 Causes Bronchopulmonary Dysplasia in Neonatal Mice
Authors: Philpot Patrick , Graumuller Fred , Melchiorre Nicole , Prahaladan Varsha , Takada Xander , Chandran Srinarmadha , Guillermo Melissa , Dickler David , Aghai Zubair H. , Das Pragnya , Bhandari Vineet
Published: May 2024

Background: Exposure to hyperoxia is an important factor in the development of bronchopulmonary dysplasia (BPD) in preterm newborns. MicroRNAs (miRs) have been implicated in the pathogenesis of BPD and provide a potential therapeutic target. Methods: This study was conducted utilizing a postnatal animal model of experimental hyperoxia-induced murine BPD to investigate the expression and function of miR-195 as well as its molecular signaling targets within developing mouse lung tissue. Results: miR-195 expression levels increased in response to hyperoxia in male and female lungs, with the most significant elevation occurring in 40% O2 (mild) and 60% O2 (moderate) BPD. The inhibition of miR-195 improved pulmonary morphology in the hyperoxia-induced BPD model in male and female mice with females showing more resistance to injury and better recovery of alveolar chord length, septal thickness, and radial alveolar count. Additionally, we reveal miR-195-dependent signaling pathways involved in BPD and identify PH domain leucine-rich repeat protein phosphatase 2 (PHLPP2) as a novel specific target protein of miR-195. Conclusions: Our data demonstrate that high levels of miR-195 in neonatal lungs cause the exacerbation of hyperoxia-induced experimental BPD while its inhibition results in amelioration. This finding suggests a therapeutic potential of miR-195 inhibition in preventing BPD.