bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparison of Cas12a and Cas9- mediated mutagenesis in tomato cells
Authors: Slaman Ellen , Kottenhagen Lisanne , de Martines William , Angenent Gerco C. , de Maagd Ruud A.
Published: October 2023

Cas12a is a promising addition to the CRISPR toolbox, offering versatility due to its TTTV-protospacer adjacent motif (PAM) and the fact that it induces double-stranded breaks (DSBs) with single-stranded overhangs. We characterized Cas12a-mediated genome editing in tomato using high-throughput amplicon sequencing on protoplasts. Of the three tested variants, Lachnospiraceae (Lb) Cas12a was the most efficient. Additionally, we developed an easy and effective Golden-Gate-based system for crRNA cloning. We compared LbCas12a to SpCas9 by investigating on-target efficacy and specificity at 35 overlapping target sites and 57 (LbCas12a) or 100 (SpCas9) predicted off-target sites. We found LbCas12a an efficient, robust addition to SpCas9, with similar overall though target-dependent efficiencies. LbCas12a induced more and larger deletions than SpCas9, which can be advantageous for specific genome editing applications. Off-target activity for LbCas12a was found at 10 out of 57 investigated sites. One or two mismatches were present distal from the PAM in all cases. We conclude that Cas12a-mediated genome editing is generally precise as long as such off-target sites can be avoided. In conclusion, we have determined the mutation pattern and efficacy of Cas12a-mediated CRISPR mutagenesis in tomato and developed a cloning system for the routine application of Cas12a for tomato genome editing.
Fractured Guidewire in Calcified Chronic Total Occlusion: Is Rotational Atherectomy Contraindicated?
Authors: Hong Xu-Lin , Zhu Jun-hui , Fu Guo-Sheng , Zhang Wen-Bin
Published: October 2023

Guidewires fracture may occur in severely calcified coronary chronic total occlusion (CTO). Rotational atherectomy (RA) is safe if the broken guidewire is firmly entrapped in the lesion. We present a case of using RA to open the calcified CTO and meanwhile grind the entrapped wire.
Association between systemic immune-inflammation index and insulin resistance and mortality: Evidence from NHANES 2011–2016
Authors: Deng xiaoqi , Liu Dichuan , Li Miao , He Jie , Fu Yufan
Published: October 2023

The role of inflammation in disease promotion is significant, yet the precise association between a newly identified inflammatory biomarker and insulin resistance (IR) and mortality remains uncertain. We aim to explore the potential correlation between systemic immune-inflammation index (SII) and these factors. We used data from 2011–2016 of National Health and Nutrition Examination Surve, and multivariate logistic regression and restricted cubic spline were employed. Subgroup and interaction analysis were conducted to recognize the consistency of the results. The association between SII and mortality was described by survival analysis. 6734 participants were enrolled, of whom 49.3% (3318) exhibited IR and 7.02% experienced mortality. Multivariate logistic regression revealed that individuals in the highest quartile (Q4) of SII had a significantly increased risk of IR compared to those in the lowest quartile (Q1). We then identified a linear association between SII and IR with an inflection point of 407, but may be influenced by gender. Similarly, compared to Q1, people whose SII at Q4 showed a higher all-cause and cardiovascular mortality. It showed a significant association between SII and both all-cause and cardiovascular mortality, but the results need to be interpreted with caution.
Causal effects between negative moods and cardiovascular diseases: a Mendelian randomization study
Authors: Yu Shuyang , Chen Songzan , Tao Tingting , Liu Xianglan , Fu Guosheng , Shen Zhida , Huang He
Published: October 2023

Background.
Previous observational studies have found that negative moods are associated with cardiovascular diseases (CVDs). However, their causal association remains unclear. We aim to evaluate the causal associations of genetically determined negative moods with CVDs including coronary artery disease (CAD), myocardial infarction (MI), stroke, and subtypes of stroke using Mendelian randomization study.
Methods.
Summary statistics for negative moods were extracted from the UK Biobank and a large genome-wide association study including 366,726 subjects. Six negative moods, tiredness, depressed mood, tenseness, unenthusiasm, anxiety, and anger, were included. Genetic associations with CVDs were extracted from the CardiogramplusC4D consortium and MEGASTROKE consortium: CAD (n = 184,305), MI (n = 171,875), stroke (n = 446,696), and subtypes of stroke. Causal effects were inferred between related traits by univariable, reverse and multivariable Mendelian randomization (MR) method.
Results.
Univariable MR analyses found that tiredness was genetically correlated with CAD (OR 1.91; 95% CI 1.23–2.97; P = 0.004) and MI (OR 2.12; 95% CI 1.29–3.47; P = 0.003). Besides, we found that the genetic liability to depressed mood was associated with higher risks of CAD (OR 2.97; 95%CI: 1.25–7.09; P = 0.014) and MI (OR 2.81; 95%CI: 1.06–7.42; P = 0.037). No causal relationship between negative moods and stroke was found. Bidirectional MR analysis failed to find any significant causal effect of CVDs on the risk of negative moods. Multivariable MR analyses revealed compelling evidence of tiredness on the risk of CAD and MI. However, after adjusting for other cardiovascular risk traits, the causal relationship between depressed mood and CVDs disappeared.
Conclusions.
This MR study found that genetic predisposition to tiredness may have a causal effect on the risk of CAD and MI. Early diagnosis and prevention of tiredness may be helpful in the management of CAD and MI.
Dihydroartemisinin inhibits EMT progression in medullary thyroid carcinoma through Hippo signaling pathway regulated by IL-6
Authors: Li Ruicong , Zhang Xinyu , Ge Yanan , Zhao Zhen , Feng Liangliang , Li Xiaoming
Published: October 2023

Dihydroartemisinin, an artemisinin derivative, has the ability to influence both the inflammatory response and the growth of certain malignancies. In this study, we used the CCK-8 and Transwell assays to show that DHA had a suppressive effect on the growth, migration, and invasion of medullary thyroid cancer cells. Furthermore, we used Elisa, Western blot, and immunofluorescence assays to confirm the expression of transcriptional co-activators YAP/TAZ downstream of the Hippo pathway, as well as changes in the expression of EMT process markers E-cadherin and N-cadherin.The results demonstrated that DHA effectively reduced the expression of IL-6 in medullary thyroid carcinoma cells and hindered their EMT process by regulating the Hippo pathway. This regulation was achieved through the promotion of YAP phosphorylation and the inhibition of YAP/TAZ protein expression.Following additional activation of the Hippo pathway with GA-017, the inhibitory effect of DHA on IL-6 was alleviated. Subsequently, the Hippo pathway was activated, leading to an increase in the expression of E-cadherin, a marker associated with the epithelial-mesenchymal transition (EMT) process. In conclusion, this study demonstrates that DHA can regulate the Hippo pathway by inhibiting IL-6 secretion, leading to the inhibition of the EMT process in medullary thyroid carcinoma. These findings provide a theoretical foundation for further exploring the anticancer mechanism of DHA. Additionally, this study offers valuable insights for the potential clinical application of DHA as a combination drug.
Changes of inflammatory mediator’s expression in the rat medial prefrontal cortex (mPFC) after minocycline treatment in lipopolysaccharide induced neuroinflammation rat model
Authors: Qaid Entesar Yaseen Abdo , Abdullah Zuraidah , Zakaria Rahimah , Long Idris
Published: October 2023

Introduction:
Minocycline has been showed can ameliorates neuroinflammation that was encountered in many neurodegenerative diseases. This study aims to investigate the expression of inflammatory mediators in the rat medial prefrontal cortex (mPFC) after minocycline treatment in lipopolysaccharide (LPS) induced neuroinflammation rat model.
Methods
Adult male Sprague Dawley (SD) rats (N = 50) were divided into 5 groups: 1) control, 2) LPS (5 mg/kg), 3) LPS + minocycline (25 mg/kg), 4) LPS + minocycline (50 mg/kg) and 5) LPS + memantine (10 mg/kg). Intraperitoneal minocycline and memantine were given daily for 14 days, while LPS injection was given once on 5th day. Western blot and immunohistochemistry were used to assess density and expression of TLR-4, nuclear factor kappa-B (NF-kB), tumour necrosis factor (TNF)-α and cyclooxygenase (COX)-2 in the medial prefrontal cortex (mPFC) of rats.
Results
Findings displayed that minocycline significantly decreased expression and density of TLR-4, NF-kB, TNF-α and COX-2 proteins that were comparable to memantine in mPFC of SD rat injected with single intraperitoneal LPS. Interestingly, the anti-inflammatory effects of minocycline 50 mg/kg were significantly more than minocycline 25 mg/kg.
Conclusion
This study suggested that minocycline can modulated LPS-induced neuroinflammation in dose-dependent manner in the mPFC area. Thus, it is suggested that minocycline can be used as potential preventive-therapeutic drug for neuroinflammatory diseases such as depression and anxiety.
A dopamine D1-like receptor-specific agonist improves the survival of septic mice while suppressing neuroinflammation
Authors: Tanaka Koichi , Choudhury Mohammed , Kikuchi Satoshi , Umakoshi Kensuke , Miyaue Noriyuki , Takenaga Ayane , Shinabe Rintaro , Mikami Kanta , Matsumoto Hironori , Yano Hajime , Nagai Masahiro , Takeba Jun , Tanaka Junya
Published: October 2023

Background
Sepsis is a leading cause of mortality in intensive-care units worldwide. It is accompanied by dysregulated systemic immune reactions, which lead to multiple organ failure. In addition to the peripheral organs, the brain is often affected by sepsis. Sepsis-induced brain dysfunction is termed sepsis-associated encephalopathy (SAE) and displays severity ranging from mild delirium to deep coma. SAE is known to increase the mortality of sepsis cases.
Methods
A murine sepsis model was prepared using the cecum ligation and puncture (CLP) technique. The expression of dopamine (DA) receptors (DRs) was examined by quantitative reverse transcription polymerase chain reaction (RT-PCR). Methyl-4-phenyl-1,2,3,6-tetrahydropyridine hydrochloride (MPTP) was used to decrease intracerebral DA levels. The blood-brain barrier (BBB)-permeable DA D1-like receptor selective agonist SKF-81297 (SKF), the BBB-impermeable D1R agonist fenoldopam, and DA were intraperitoneally administered shortly after CLP. CLP-induced changes in body temperature and mortality were then monitored every 12 h. Systemic and brain inflammation were investigated using flow cytometry, quantitative RT-PCR, and an enzyme-linked immunosorbent assay. SAE-like symptoms were evaluated using behavioral tests and electroencephalogram (EEG)/electromyogram (EMG) recordings.
Results
D1-like receptors were significantly expressed in the brain but not in the lung. MPTP aggravated mortality in septic mice. SKF, but not fenoldopam, prevented CLP-induced death of septic mice. SKF ameliorated acute lung injury-like pathology and inhibited leukocyte accumulation in the lung and in the brain. SKF suppressed the expression of TNFα and IL-1β in the periphery and brain. The Morris water maze and Y-maze tests revealed that CLP-induced cognitive dysfunction was ameliorated by SKF, and EEG/EMGs showed that SKF increased sleep duration after CLP.
Conclusion
These data suggest that endogenous DA prevents sepsis-induced neuroinflammation through D1-like receptors, and BBB-permeable D1-like receptor agonists can be novel drugs for treating sepsis. Neuroinflammation that causes SAE may be a critical cause of sepsis-induced death.
Distribution of Staphylococcus species and Bacillus species in environment of dairy farm located at sewerage site, river Ravi and urban area, Lahore, Pakistan
Authors: Khan Tanveer Ali , Afzal Hanan , Noreen Amara , Ali Zulfiqar , Mustafa Ghulam
Published: October 2023

Bioaerosol contains many pathogenic species of bacteria. The workers of dairy farms are continuously exposed to the farm environment so they are more prone and susceptible to airborne infections. In the current study, different samples from the dairy farm of three regions viz sewerage site, river Ravi and urban areas were collected. The bacterial load was accessed and also some pathogenic bacteria were isolated. The 16S rRNA gene characterization analysis followed by the phylogenetic tree analysis showed 98–99% similarities with the staphylococcus sciuri, bacillus toyogensis and bacillus pulmis. The overall microbial load was higher in open and sewerage site farms as compared to the open farm of river Ravi and urban area farm. Similar trend was observed in closed farms. The 16s rRNA gene characterization and phylogenetic analysis not only help to identify species level identification but also genetic relatedness with other species. So, farm’s worker’s health is more at risk at open and sewerage site farm than river Ravi and urban area farms.
Complex Interplay of Psychiatry Disorders, Fractures, and Osteoporosis: A Mendelian Randomization Study
Authors: wu xueqiang , Li zefang , li hanzheng , bi cong , zhang can , sun yiqing , Yan Zhaojun
Published: October 2023

Background
Neurodevelopmental disorders, such as Attention-Deficit/Hyperactivity Disorder (ADHD), Autism Spectrum Disorder (ASD), and Tourette Syndrome (TS), have been extensively studied for their multifaceted impacts on psychological, social, and emotional well-being. Recently, there has been growing interest in their potential relationship with fracture risks in adulthood. This study aims to explore the associations between these disorders and fracture rates, considering factors like self-harm and the impact of psychotropic medications.
Methods
Employing a novel approach, this study utilized Mendelian randomization (MR) analysis to investigate the complex interplay between ADHD, ASD, TS, and fractures. The MR framework, leveraging extensive genomic datasets, facilitated a systematic examination of potential causal relationships and genetic predispositions.
Results
The findings unveil intriguing bidirectional causal links between ADHD, ASD, and specific types of fractures. Notably, ADHD is identified as a risk factor for fractures, with pronounced associations in various anatomical regions, including the skull, trunk, and lower limbs. Conversely, individuals with specific fractures, notably those affecting the femur and lumbar spine, exhibit an increased genetic predisposition to ADHD and ASD. These results provide a genetic perspective on the complex relationships between neurodevelopmental disorders and fractures, emphasizing the importance of early diagnosis, intervention, and a holistic approach to healthcare.
Conclusion
This research sheds new light on the intricate connections between neurodevelopmental disorders and fractures, offering valuable insights into potential risk factors and causal links. The bidirectional causal relationships between ADHD, ASD, and specific fractures highlight the need for comprehensive clinical approaches that consider both psychological and physical well-being. Early diagnosis, pharmacological interventions, and diligent monitoring of bone health are essential components of improved healthcare strategies for individuals with ADHD and ASD.
Seasonal and ecological determinants of wild boar rooting on priority protected grasslands
Authors: Calosi Martina , Gabbrielli Chiara , Lazzeri Lorenzo , Fattorini Niccolò , Cesaretti Gloria , Burrini Lucia , Petrillo Ottavio , Ferretti Francesco
Published: October 2023

Wild ungulates can influence various trophic levels, regulating carnivore abundance as well as affecting habitat structure. Conservation problems can arise when high ungulate densities threaten species or habitats with conservation concern. Assessing factors influencing the intensity of their impact is important to identify appropriate measures enhancing the conservation of protected habitats. We assessed seasonal and ecological factors influencing wild boar Sus scrofa pressure on EU priority grasslands in three protected areas of central Italy, by modelling the effects of habitat/topography-related variables, as well as wild boar population density, on indices of rooting activity. We seasonally surveyed 136 sampling plots from spring 2019 to spring 2021 to estimate rooting, and we used faecal counts to estimate wild boar densities in summer. Estimates of density and rooting varied from 3.5 to 22.2 individuals/km2 and from 1.5 to 22.2% of rooted ground, respectively. We detected a clear seasonal trend in rooting activity, that peaked in autumn and winter. We also found a positive correlation between spring-summer rooting and summer density, across sites. Rooting intensity was negatively related to the local extent of rock cover and increased with the size of the grassland patch and with the percentage of forest around each plot. These results emphasise the wild boar tendency to exploit feeding sites in ecotonal areas, i.e., at the interface between forest and meadows, which maximises security and ease of finding food resources. Actions aiming at the protection of focal plants in grassland habitats, as well as reducing wild boar densities, are supported.
Myopia Progression during COVID-19 Home Quarantine in Indonesia: A Retrospective Multicenter Cohort Study
Authors: Sitorus Rita S , Caesarya Sesy , Karfiati Feti , Manurung Florence M , Ekowati Liana , Sumual Vera , Loebis Rozalina , Darusman Kianti Raisa , Surasmiati Ni made Ayu , Sayuti Kemala , Aldy Fithria , Wulandari Lely Retno , Rahmah Marlyanti Nur , Estu Dian , Barliana Julie Dewi , Prabaniswara Marcelius Patria , Sutandi Nathania , Widyahening Indah Suci
Published: October 2023

Purpose: To evaluate the impact of home quarantine on myopia progression in Indonesian children during COVID-19 pandemic.
Methods: This multicenter retrospective cohort study included children aged 8-17 years old from 20 hospitals across 9 provinces in Indonesia. Medical records of children diagnosed with myopia and/or astigmatism were obtained and grouped to pre-pandemic (September 2019-March 2020) and pandemic periods (September 2021-May 2022). Patient demographics and spherical equivalents of refraction (SER) were collected. Direct or phone interviews were conducted with questions regarding the child’s screen time, outdoor activity time, break time in between gadget use, and sleep duration.
Results: In total, 607 children were enrolled. There was a significant increase in SER progression (-0.97 ± 1.04 D, p<0.001). SER progression in Java-Bali was higher compared to others (-1.3± 1.18 D vs 0.57± 0.67 D, p<0.001). A total of 262 patients were interviewed. Younger age (OR: 2.52, 95% CI:[1.42-5.03], p = 0.004), female gender (OR: 2.16, 95% CI:[1.18-3.83], p = 0.011), screen time of ≥4 hours daily for leisure activities (OR: 1.95, 95% CI:[1.07-3.54], p = 0.028), ≤1 hour of time spent outdoor daily (OR: 1.89, 95% CI:[1.03-3.47], p = 0.047, and sleep duration of >8 hours (OR: 2.7, 95% CI:[1.46-4.99], p = 0.002) were significantly associated with fast myopia progression.
Conclusion: Myopia progression was accelerated during COVID-19 home quarantine, especially in more urban area. Collaborative efforts should be made to address this current issue.
Tetra-primers ARMS-PCR-based analyses of the involvement of intronic variation in the FTO gene for the risk of developing knee osteoarthritis
Authors: Khan Bushra , Kumar Ranjeet , Sahar Najmus , Saifullah Saifullah
Published: October 2023

Genetic polymorphism in FTO was reported to disturb energy homeostasis, mediating obesity that may lead to OA. Therefore, the study aims to detect the presence of the FTO genetic variant, rs8044769, in patients of OA and to analyze the association of the genetic polymorphism with the disease. Case-control research was conducted at the University of Karachi between March to September 2022. Diagnosed OA patients, based on the ACR criteria, were enrolled from the T.O. Clinic and SouthCity Hospital, Karachi, Pakistan. Blood samples were collected from 125 OA patients and 125 healthy individuals with informed consent. Genomic- DNA was isolated, and genetic variations were analyzed. A significant association was observed between rs8044769 genotypes and susceptibility to OA (χ2 = 7.82, df = 2, P < 0.05). The minor allele of the FTO-rs8044769 had a significant association with the disease risk among the studied participants (OR = 1.729, 95% CI = 1.214 − 2.464, P = 0.002). In the female cohorts, rs8044769 conferred the disease susceptibility at the co-dominant, CC v/s TT (OR = 2.71, 95% CI = 1.09 − 6.76, P = 0.03); recessive, TT v/s CC + CT (OR = 2.17, 95% CI = 1.03 − 4.56, P = 0.04); additive, 2TT + CT v/s 2CC + CT (OR = 1.65, 95% CI = 1.05 − 2.61), P = 0.02) and allelic C v/s T (OR = 1.81, 95% CI = 1.11 − 2.96, P = 0.01) levels. There is a positive association of the FTO gene polymorphism with disease development in OA patients.
Spatial distribution and health risk assessment of soil petroleum hydrocarbons at an oil refinery in northwest China
Authors: Bai Jinjing , Jiang Jing , Yan Zengguang , Wang Qihao , Zhang Chao , Yang Pingguo , Yang Min
Published: October 2023

In the present study, the concentrations of six petroleum hydrocarbons, including n-alkane hydrocarbons (total petroleum hydrocarbons [TPH] (C6–C9) and (C10–C40)), BTEX(benzene, toluene, ethylbenzene, and m- and p-xylene), in 261 soil (0–14 cm), 31 groundwater, and 4 soil gas samples were measured to determine their spatial distribution and human health risks. The study integrated the model by Johnson and Ettinger and the HJ 25.3–2019 technical standards for risk assessment of soil contamination with localized parameters using Chinese demographic characteristics and site features to determine Health risk assessments. The results revealed that TPH (C6–C9) was the main contaminant in oil refineries, and the m- and p-xylene concentrations exceeded the screening values in groundwater and soil gas but not in soil. According to the spatial distribution characteristics, the contaminants were mainly concentrated in the refining workshop and around the storage tank, and the migration ability of linear-chain petroleum hydrocarbons in the soil was stronger than that of BTEX. TPH (C6–C9), TPH (C10–C40), and benzene in soil and groundwater pose a threat to human health. The pathway of steam invasion in soil gas and the carcinogenic risk and hazard quotient assessment of benzene in soil gas were 0–4 and 1–3 orders of magnitude higher than those in soil. Aliphatic hydrocarbons [AHCs] (C10–C12) had the highest non-carcinogenic risk among all TPH (C10–C40) parts. The present study provides a reference to accurately determine the toxicological parameters of TPH (C6–C9) and TPH (C10–C40) and to improve the BTEX risk assessment methods.
Chromosomes and the Mitotic Cell Cycle Phase in Onion Roots
Authors: Gupta Shalini
Published: October 2023

Onions (Allium cepa) are commonly employed in vitro to evaluate the effects of chemical agents. Onion chromosomes have long been prepared using the acetic orcein staining technique. However, aceto-orcein stain is corrosive and deadly since it contains oxidizing agents like organic peroxides, toxic compounds like cyanides, acid corrosives, and radioactive materials. This study examined the mitotic activity in the roots of onion plants to ascertain the impact of the aceto orcein dye's soaking period on actively dividing root cells. Using the aceto-orcein squash procedure, a number of root tips from each bulb were collected, soaked for 1, 3, and 24 hours in aceto-orcein stain, and then processed further for cytological analyses. The purpose of the research was to examine the impact of chromosomal aberration and mitotic index on onion roots. It will reveal how many cells are actually going through mitosis. The tip of onion root cells was observed going through mitosis while actively undergoing mitotic division using the usual methods. There are various phases involved in mitotic divisions, including prophase, metaphase, anaphase, telophase, and interphase. At each soaking time, the experiment was run six times. The outcome shown that different soaking time durations had a substantial impact on the decline in mitotic index value.
SMART TECHNOLOGIES ONTHE TWO WHEELS POLE ASSEMBLY LINE: CHALLENGES, OPPORTUNITIES, AND IMPLICATIONSOF PYTHON-BASEDAUTOMATION
Authors: Pedro Rocha Pereira Joao , Eduarda Sanchez Gomes Luize , de Souza Torres Mayara , Mark Lobo de Oliveira Jean , Augustoda Paz Elleres Pablo
Published: October 2023

This article addresses the implementation of intelligent automation in the assembly line of Polo Duas Rodas, using the PDCA methodology, aiming to optimize production and quality processes. Based on the convergence between industrial automation and advanced artificial intelligence, machine learning and Industrial Internet of Things (IIoT) technologies, the assembly line has been transformed to meet the demands of Industry 4.0. Through the stages of the PDCA cycle, objectives were established, automation technologies were implemented, results were evaluated in real time and adjustments were made for continuous improvements. The results of the tests demonstrated significant improvements in production efficiency, product quality and adaptation to changes. The approach adopted has reinforced the assembly lines ability to adapt to the ever-evolving industrial landscape, highlighting its competitive position in the era ofintelligentautomationandIndustry 4.0.
AGRONOMY OF MULATO GRASS CULTIVAR (BRACHIARIA SP.) IN GRAZING CONDITIONS IN QUINTANA ROO, MEXICO
Authors:   Campo Experimental Chetumal, km 25 Carretera Chetumal - CancÃƒÂºn s/n. Xul-ha, Quintana Roo., Enrique Sosa Rubio Edgar , Herrera Cool Gilbert ,   Campo Experimental Chetumal, km 25 Carretera Chetumal - CancÃƒÂºn s/n. Xul-ha, Quintana Roo., del Carmen Zavaleta Cordova Maria ,   Campo Experimental Chetumal, km 25 Carretera Chetumal - CancÃƒÂºn s/n. Xul-ha, Quintana Roo., Alcaraz Romero Rosendo ,   Campo Experimental Mococha. INIFAP-CIRSE.
Published: October 2023

The objective of this study was to determine the behavior of Mulato grass under grazing conditions in Quintana Roo. This work was carried out in the Chetumal Experimental Field of INIFAP in Quintana Roo, which is located 25 km from Chetumal, in the vicinity of the Juan Sarabia ejido, on km 25 of the Chetumal federal highway. For the development of the study, two hectares were used, 1 ha of Brachiaria humidÃƒÂ­cola (Chetumal) and another of Brachiaria sp. (mulato). For each type of pasture, three recently weaned steers of the Holstein for Zebu and Swiss for Zebu breeds with an approximate initial weight of 242 Ã‚Â± 3 kg were used the variables measured were seasonal dry matter production, plant cover and height, forage supply, and the botanical composition of the meadow. Subsequently, the steers were changed by load adjustment and the weight gain was determined. The design used was totally randomized with three repetitions per treatment and the analysis of the information was carried out through the ANOVA. No differences (PÃ¢Â‰Â¥0.05) were found in the number of plants 12 weeks after establishment. For the coverage parameter, differences (PÃ¢Â‰Â¤0.05) were observed with values Ã¢Â€Â‹Ã¢Â€Â‹of 89% and 77 for Chetumal and mulatto grass, respectively. The values Ã¢Â€Â‹Ã¢Â€Â‹of weight gain among the grasses under study (650 vs 675 for Chetumal and mulatto, respectively) did not indicate differences (PÃ¢Â‰Â¥0.05) between them. Therefore, mulatto grass should be considered as an option for livestock in the state.
ASSESSING DISEASE SEVERITY AND QUALITY OF LIFE IN PSORIASIS PATIENTS: A COMPREHENSIVE STUDY
Authors:   Department of Clinical Pharmacy Practice, Vaageswari College of Pharmacy, Telangana-India., Anoosha Dr. B. , Banu Shagufta ,   Department of Clinical Pharmacy Practice, Vaageswari College of Pharmacy, Telangana-India., Tarannum Syeda ,   Department of Clinical Pharmacy Practice, Vaageswari College of Pharmacy, Telangana-India., Alia Tabassum Syed ,   Department of Clinical Pharmacy Practice, Vaageswari College of Pharmacy, Telangana-India., Taskeen Nishat ,   Department of Clinical Pharmacy Practice, Vaageswari College of Pharmacy, Telangana-India.
Published: October 2023

Psoriasis, a common and chronic non-contagious skin condition, presents as red, scaly, and itchy patches that significantly impact patients lives. This hospital-based prospective observational study, spanning six months in the outpatient department, aimed to assess disease severity and its effect on quality of life among psoriasis patients. Disease severity was determined using the Psoriasis Area and Severity Index (PASI) scale, while the Dermatological Life Quality Index Scale (DLQI) measured its impact on daily functioning. Of the 110 patients screened, the 36-55 age group dominated, representing individuals aged 15 to 56 and above. Gender distribution revealed 60.9% males and 39.1% females, with males predominantly affected, particularly by chronic plaque psoriasis and Palmoplantar psoriasis. PASI assessments indicated that 39.0% of patients suffered from severe disease, while DLQI assessments revealed moderate quality of life impact in 50.9% of cases. In summary, this study identified age, gender, disease duration, and psoriasis type as significant determinants of patients experiences in the dermatological department. Importantly, a clear association emerged between disease severity and decreased quality of life, influenced by various contributing factors. These findings underscore the importance of comprehensive care and support, acknowledging the multifaceted nature of psoriasis management to enhance overall patient well-being.
A PROSPECTIVE STUDY OF FUNCTIONAL OUTCOMES FOR CLOSED FRACTURE PROXIMAL HUMERUS TREATED WITH EXTERNAL FIXATOR
Authors: Banerjee Sanjeev , Kumar Pradhan Chinmaya , Varma H.S. , Naik Sparsh
Published: October 2023

Background:In the aging population, the proximal humerus fracture is the third most common osteoporotic fracture type after hip and distal radius fractures, accounting for 4% of all fractures. More than 70% of the patients that present with proximal humerus fracture are over 60 years of age and 75% are women. The mechanism of low-energy injury in elderly patients is usually falling from standing height. Aims And Objective: To study the functional outcomes for closed fracture proximal humerus treated with external fixator. Materials And Methods:A prospective study using a clinical and X-ray assessment of 86 two-and three-part fracture of the humerus in patients that underwent external fixation. Result:Mean scores of points were obtained using the DASH SCORE, with 80% satisfactory results. The following variables did not influence results: sex (p >0.05), age (p >0.05), laterality (p >0.05), nor type of fracture (p >0.05). Union was obtained in all cases. Reduction was considered good in 70(81%) cases, there were 11 cases of union in varus and 4 in valgus. Conclusion:This procedure makes it possible to obtain early satisfactory functional results and helps rehabilitation as it limits postoperative mobility to a lesser degree than other techniques it is also less aggressive than open reduction and internal fixation and has a low complication rate.
COMPREHENSIVE EVALUATION OF PREGNANCY COMPLICATIONS AT A TERTIARY CARE TEACHING HOSPITAL
Authors:   Department of Clinical Pharmacy Practice, Vaageswari College of Pharmacy, Telangana-India., Fareed Sana , Saba Iram ,   Department of Clinical Pharmacy Practice, Vaageswari College of Pharmacy, Telangana-India., Lingampally Srujana ,   Department of Clinical Pharmacy Practice, Vaageswari College of Pharmacy, Telangana-India., Kumar Dr. R. Dinesh ,   Department of Clinical Pharmacy Practice, Vaageswari College of Pharmacy, Telangana-India.
Published: October 2023

Pregnancy complications, while often mild, can pose significant risks to both mother and child. Common complications include anemia in pregnancy, thyroid disorders, gestational hypertension, preeclampsia, eclampsia, gestational diabetes mellitus (GDM), infections, and preterm labor. Physiological changes during pregnancy increase the demand for iron, making anemia a prevalent concern. Preeclampsia, characterized by elevated blood pressure and proteinuria, presents a risk with an unknown etiology. Timely prenatal care and early detection are essential for mitigating these risks. This prospective observational study was conducted over six months in an obstetrics ward at a tertiary care teaching hospital, involving a sample of 110 participants. Our findings revealed that the 18-25 age group was most susceptible to pregnancy complications. Moreover, the majority of complications were detected during the third trimester, affecting 74.54% of the study population. Among the subjects, 25.45% experienced hypertensive disorders, 20% were diagnosed with GDM, 13.63% suffered from anemia during pregnancy, and 36.36% reported thyroid disorders during pregnancy. Notably, thyroid disorders were the most frequently identified complication in this population. Complications during pregnancy can have severe maternal and fetal consequences if left untreated. Physiological changes, inadequate antenatal care, young age at pregnancy, illicit drug use, and multiple pregnancies are contributing factors to these complications. Early screening, diagnosis, and treatment are crucial for effective management and the prevention of severe maternal and fetal complexities. Educating and raising awareness about symptoms, consequences, and preventive measures play a vital role in reducing the occurrence of pregnancy complications.
MANAGEMENT OF FUSED PRIMARY TEETH IN ESTHETIC ZONE A CASE REPORT
Authors: M.S Yadukrishnan , Zarina A. Rita , J. Reshmi , Dayakar P.S Danu
Published: October 2023

Tooth fusion arises through union of two normally separated tooth germs. Depending upon the stage of development of the teeth at the time of union, it may be either complete or incomplete and is more often seen in deciduous dentition. This article discusses esthetic oriented endodontic management of fused maxillary anterior in a 4-year-old child.
OPTIMIZATION OF CASSAVA EFFLUENT BIOMETHANIZATION WITH ASH AS COSUBSTRATE
Authors:   AgrovalorisationLaboratory, Biochemistry and MicrobiologyDepartment, Universite Jean Lorougnon Guede, BP 150 Daloa (Cote dIvoire)., Nazo Edith Kpata-Konan , Francis Kouame ,   Environmental Sciences and Technologies Laboratory, Physics Chemistry Mathematics Computer Science DepartmentUniversite Jean Lorougnon Guede, BP 150 Daloa (Cote dIvoire)., Tanon Virginie NGBADJO ,   AgrovalorisationLaboratory, Biochemistry and MicrobiologyDepartment, Universite Jean Lorougnon Guede, BP 150 Daloa (Cote dIvoire).
Published: October 2023

The main objective of this study is to determine the optimal conditions for anaerobic biodigestion of cassava effluents with the ash of cassava peels as co-substrate. To do this, three (03) digesters were used in which were put effluent + ash for digester 1, effluent + ash + cow dung for digester 2 and effluent + ash + urine for digester 3. The physico-chemical parameters (temperature, pH), biochemical parameters (COD and TKN) and the volume of biogas produced were quantified. In each of the reaction media a decrease in values was observed in pH (digester 1: 7.15 to 6.92 digester 2: 7.20 to 5.38 digester 3: 7.00 to 5.28), COD (digester 1: 1.66g/l to 1.16g/l digester 2: 1.81g/l to 1.44g/l digester 3: 1.46g/l to 0.99g/l) and NTK (digester 1: 0.27 g/l to 0.24 g/l digester 2: 0.29 g/l to 0.22 g/l digester 3: 0.25 g/l 0.16 g/l). Â¨For the temperature of the media, the recorded values are in the range of mesophilic fermentation (digester 1: 26.66Â°C, digester 2: 27.97Â°C and digester 3: 27Â°C). Regarding gas production, the digesters produced gas from day 1 with 37.463 m3, 37.463 m3 and 69.272 m3 respectively. A significant improvement in the biogas production rate was observed in all digesters. Thus, the ash could be used as a replacement for human urine for pH adjustment of cassava effluents.
CYTOTOXIC AND GENOTOXIC EFFECTS OF UV IRRADIATION ON ROOT MERISTEM CELLS OF ALLIUM CEPA L
Authors:   Department of Botany, Miranda House, University of Delhi, Delhi 110007., Bajaj Madhu , Bahri Saloni ,   Department of Botany, Miranda House, University of Delhi, Delhi 110007., Agrawal Renuka ,   Department of Botany, Miranda House, University of Delhi, Delhi 110007., Sinha Roy Somdutta ,   Department of Botany, Miranda House, University of Delhi, Delhi 110007., a Riya ,   Department of Botany, Cluster University of Jammu, Jammu 180001., Khanna Charvi ,   Zakir Hussain Delhi College, University of Delhi, New Delhi 110002.
Published: October 2023

The widespread use of ultraviolet radiation as a disinfectant necessitates an investigation into its possible harmful effects in living organisms. In the present study the effect of exposure to UVC radiation was studied using the Allium cepa assay. To determine the effect of UVC radiation, roots of four sets of A. cepa bulbs were irradiated with 254 nm UV radiation for durations of half, one, two and four hours, respectively. The cytotoxicity of UVC radiation was assessed by comparing the mitotic index of root meristem cells of exposed roots and unirradiated roots (control). Frequency of chromosomal aberrations in the dividing cells of the root meristem was taken as a measure of the genotoxicity of UVC radiation. The roots subjected to UVC exposure showed several types of chromosomal aberrations including sticky chromosomes, c-metaphase, vagrant chromosomes, irregular anaphase, multipolar anaphase, chromosomal bridges and laggards. The study clearly shows that exposure to UVC radiation for even a brief period of half an hour is cytotoxic and genotoxic.
RESPONSES OF RMG PRODUCERS IN BANGLADESH TO MULTINATIONAL BUYERS DEMAND DURING COVID-19 PANDEMIC
Authors: Al-Amin Syeedul , Mohiuddin Muhammad
Published: October 2023

The COVID-19 pandemic affected Bangladesh RMG sectors negatively and the sector producers have undertaken policy and action plans to overcome the setbacks to survive and sustain in the wake of declining buyersÃ¢Â€Â™ demands. Drastic fall of demand, order cancelation, reduction of the number of orders, payment holding up, order price fall, etc. were the effects that sharply declined the RMG situations. The study found that mainly two policies were taken by the RMG owners to handle the crises. They are the Cost reduction policy and the new market search policy. Under such policies, many action measures were taken. Besides, governmental support helped a lot. All these actions at last ended up with the survival of many firms. Till now they are struggling with the crises. Mixed-form research methods have been applied in the study. The study was confined in the Gazipur district of Bangladesh and 20 firms working there were the sample. Further study needs to be done to know about the scenario and effects in detail.
ASSESSMENT OF QUALITY IN OBSTETRIC CARE IN CHETTINAD HOSPITAL
Authors: Logitha R. , Kumar Shery Angel Raja
Published: October 2023

Aim:To assess the quality of Obstetric Care in Chettinad Hospital for antenatal patients and adhere to practices that benefit of the patient. Design: Cross Sectional Study Methodology: Sample Size: 100 patients Inclusion Criteria: Antenatal patients. Procedure: The study is a questionnaire based study. The study population will be antenatal patients who receive antenatal care in Chettinad Hospital. After getting informed consent, the procedure will be explained thoroughly to the participants and only the participants who are willing to take part in the study will be selected. The participants were observed on an outpatient basis and those who were admitted in the antenatal ward. They were asked to answer a questionnaire. The questionnaire contains questions related to the WHO recommendations of Quality Care for Antenatal Women which includes blood pressure measurement, blood test, urine test, informed on possible complications, treatment of any such complications, counseling on nutrition, patient - doctor confidentiality, mental counseling and advice on birth preparedness plan during pregnancy. This study is completely a non-invasive study. Study Duration: 2 Months Results:The study population included 100 participants who received outpatient treatment and admitted for antenatal care in a tertiary care hospital where they received quality Antenatal care. It was observed that women who were treated with proper care and follow-up tend to entrust the doctors effectively, follow up on reviews, investigations and properly adhere to the treatment plan if recommended. The participants did not express any concerns related to the process of delivery as they were counselled accordingly, complications treated appropriately and parents adhering to the advice of the doctor. Conclusion:Providing Quality Maternity Care to antenatal patients has more positive outcomes in antenatal periods of a patient. It reduces occurrence of common antenatal complications and promotes maternal and infants well-being by reducing maternal and infant morbidity and mortality rate.
RARE CASE OF ESCITALOPRAM INDUCED THROMBOCYTOPENIA- A CASE REPORT
Authors:   Senior Resident in Internal Medicine, TD Medical College, Alappuzha., H. Sreeraj
Published: October 2023

Drug-induced thrombocytopenia can be caused by dozens, perhaps hundreds of medications. Because thrombocytopenia can have many other causes, the diagnosis of drug-induced thrombocytopenia can easily be overlooked. In acutely ill, hospitalized patients, drug-induced thrombocytopenia can be overlooked because thrombocytopenia is attributed to sepsis, the effect of coronary-artery bypass surgery, or some other underlying condition. Although drug-induced thrombocytopenia is uncommon, it can be devastating. Heparin-induced thrombocytopenia is the most common drug-related cause of drop in the platelet count. Because heparin is often given together with certain drugs that are likely to cause drug-induced thrombocytopenia (platelet inhibitors and vancomycin), it is important to distinguish between heparin-induced thrombocytopenia and drug induced thrombocytopenia. Drug-induced platelet destruction is usually caused by drug-induced antibodies, but this can be difficult to prove. Although platelets are the preferred targets of drug-induced antibodies, drugs can also cause immune hemolytic anemia and neutropenia through similar mechanisms.
DETERMINANTS OF THE EFFECTIVENESS OF AUDIT PROCEDURES IN REVEALING FRAUD: ATTRIBUTION THEORY APPROACH WITH TIME PRESSURE AS AN INTERVENING VARIABLE
Authors:   Master of Accounting, Trisakti University, Indonesia., Ifana Firdia , a Reskino ,   State Islamic University Syarif Hidayatullah Jakarta, Indonesia.
Published: October 2023

This study aims to examine the determinants of the effectiveness of audit procedures in revealing fraud, which consists of five variables: independence, objectivity, professional skepticism, ability, and auditor experience. In addition, time pressure is placed as an intervening variable. This research is quantitative with a survey of auditors at the BPK Head Office in Jakarta. An intervening model for the research framework was developed to investigate the role of time pressure intervention. This research provides evidence that the determinants of the effectiveness of audit procedures in revealing fraud in the form of independence, objectivity, professional skepticism, ability, and experience have not been fully proven in predicting the effectiveness of audit procedures in revealing fraud in Indonesia. Independence has a negative effect, while objectivity, professional skepticism, ability, and auditors experiencepositively affect the effectiveness of audit procedures in revealing fraud. However, not all of them are significant. Then, the independence and ability of auditors have a negative effect on time pressure, while objectivity, professional skepticism, and experience of auditors have a positive effect on time pressure. Testing the effect of time pressure on the effectiveness of audit procedures in revealing fraud results have a positive but insignificant effect. 5 (five) determinant variables of the effectiveness of audit procedures in revealing fraud are not proven to increase the effectiveness of audit procedures in revealing fraud through time pressure. This can be explained because this research is the first study to examine time pressure as an intervening variable on the effectiveness of audit procedures in revealing fraud, so the results cannot be compared with previous research, which partially used time pressure as a moderating variable in fraud detection.
INTERNET ADDICTION AND ITS EFFECTS ON LIFE STYLE OF THE ADOLESCENTS
Authors:   Tutor (Govt. College of Nursing, Indore), Ex-Professor & HOD of Child Health Nursing Department, Peoples College of Nursing & Research Centre, Bhopal, Madhya Pradesh, India., Bairagi Purnima , Prasad Karesh ,   Principal, Peoples college of Nursing & Research Centre, Bhopal, Madhya Pradesh, India.
Published: October 2023

Introduction:- Adolescence is a period of the lifecycle when individuals are managing multiple and complex developmental tasks. Negative impact of internet addiction which effects adolescentÂ’s behavior, academics, working performance, family life, social relationships, physical health, and psychological well being. Statement of the Problem: Â“A study to assess the effectiveness of Structured Teaching Programme (STP) regarding internet addiction and its effects on life style of the adolescents at selected schools of Bhopal, Madhya Pradesh. Methodology:-Quantitative approach was adopted and pre experimental one group pre- test and post-test study design was used. Multistage Stratified Simple Random Sampling technique was used to select the 1080 adolescents from selected urban private English medium Co. Ed higher secondary schools of Bhopal. Self Structured Questionnaires was used to collect the data and Structured Teaching Program (STP) was administered. Data analysis was done by Frequency and percentage distribution for socio demographic variables. Chi Square test was used to find out the association between pre-test knowledge and socio demographic variables. The Paired T Test was used to find the difference between pre test and post test knowledge. Statistical analysis of data done by using SPSS 20.0 Software. Results:- Results of the study revealed that majority of the variables like age, class, monthly family income and family type are significantly associated with pre test level of knowledge regarding internet addiction & its effects on life style of the adolescents. Pre-test knowledge regarding internet addiction prior to the administration of STP 543(50.3%) almost half of the participants had poor knowledge, 528(48.9%) had average knowledge and only 09(0.8%) had good knowledge. Post test knowledge regarding internet addiction after administration of STP 60 (5.6 %) had average knowledge and majority 1020 (94.4%) had good knowledge. The mean pre test knowledge score was 8.4481 (S.D = 2.39769) and mean post-test knowledge score was 18.6889 (S.D = 1.962245). Findings of comparison between pre- test and post-test knowledge regarding internet addiction & its effects on life style of the adolescents indicated highly significant (<0.0001*) difference. Conclusion:- The study findings highlighted that pre test and post test knowledge of participants having highly significant (<0.0001*) difference .Based on the findings this study concluded that majority of the adolescents knowledge improved after STP regarding internet addiction & its effects on life style of the adolescents.