bluuebunny/crossref_metadata_2025 huggingface dataset:
Chitotriosidase, a biomarker of Amyotrophic Lateral Sclerosis accentuates neurodegeneration in spinal motor neurons through neuroinflammation.
Authors: Varghese Anu Mary , Ghosh Mausam , Bhagat Savita Kumari , Vijayalakshmi K , Preethish-Kumar Veeramani , Vengalil Seena , Chevula Pradeep-Chandra-Reddy , Nashi Saraswati , Polavarapu Kiran , Sharma Meenakshi , Dhaliwal Rupinder Singh , Philip Mariamma , Nalini Atchayaram , Alladi Phalguni Anand , Sathyaprabha Talakad N , Raju Trichur R
Published: January 2020

Background Cerebrospinal fluid from Amyotrophic Lateral Sclerosis patients (ALS-CSF) induces neurodegenerative changes in motor neurons and gliosis in sporadic ALS models. Search for identification of toxic factor(s) in CSF revealed an enhancement in the level and enzyme activity of chitotriosidase (CHIT-1). Here, we have investigated its upregulation in a large cohort of samples and more importantly its role in ALS pathogenesis in a rat model. Methods CHIT-1 level in CSF samples from ALS (n=158), non-ALS (n=12) and normal (n=48) subjects were measured using ELISA. Enzyme activity was also assessed (ALS, n=56; non-ALS, n=10 and normal-CSF, n=45). Recombinant CHIT-1 was intrathecally injected into Wistar rat neonates. Lumbar spinal cord sections were stained for Iba1, Glial Fibrillary Acidic Protein and Choline Acetyl Transferase to identify microglia, astrocytes and motor neurons respectively after 48 hrs of injection. Levels of tumor necrosis factor-α and interleukin-6 was measured by ELISA. Findings CHIT-1 level in ALS-CSF samples was increased by twenty folds and it can distinguish ALS patients with a sensitivity of 87% and specificity of 83·3% at a cut off level of 1405·43 pg/ml. Enzyme activity of CHIT-1 was also fifteen folds higher in ALS-CSF and has a sensitivity of 80·4% and specificity of 80% at cut off value of 0·1077989μmol/μl/min. Combining CHIT-1 level and activity together gave a positive predictive value of 97·78% and negative predictive value of 100%. Administration of CHIT-1 increased microglial numbers and astrogliosis in the ventral horn with a concomitant increase in the levels of pro-inflammatory cytokines. Amoeboid shaped microglial and astroglial cells were also present around the central canal. CHIT-1 administration also resulted in the reduction of motor neurons.Conclusions CHIT-1, an early diagnostic biomarker of sporadic ALS activates glia priming them to attain a toxic phenotype resulting in neuroinflammation leading to motor neuronal death.
Community health volunteers’ contribution to Tuberculosis patients notified to National Tuberculosis program in Kenya
Authors: Abongo Tabitha , Ulo Benson , Karanja Sarah
Published: January 2020

Background Contact investigation is important in finding contacts who have Tuberculosis (TB) disease so that they can be given treatment and stop further transmission. The main objective of this study was to assess the contribution of community health volunteers (CHVs) to the number of TB patients notified to the National program in Kenya. The specific objectives were to establish the total number of households of TB index cases visited by CHVs for contact screening, identify the total number of household members screened for TB and establish the number of persons referred for further TB investigation and the number identified with TB.Methods This was a retrospective desk review of project reports submitted to Amref Health Africa in Kenya by the sub recipients implementing activities in the 33 counties with Case Notification Rate (CNR) of less 175/100,000 and Treatment Success Rate (TRS) of less than 88% as per the National strategic plan 2015-2018. Data for this study covered a time period between January and December 2016. Data on the notified TB patients was obtained from the National Tuberculosis Information Basic Unit (TIBU). The study population included all the TB index cases whose households were visited by CHVs for contact screening. Data was analyzed descriptively.Results CHVs visited a total of 26,307 TB patients in their households for contact screening. A total of 44,617 household members were screened for TB with 43,012 (96.40%) coming from households of bacteriologically confirmed TB patients and 1,606 (3.60%) from households of children under 5 years. The proportion of the persons referred in relation to number screened was 19.6% for those over 5 years and 21.9% from under 5 years with almost the same percentages for male and female at 19.2% and 19.7% respectivelyConclusions This study showed that in the 33 counties of Global Fund TB project implementation the percentage of TB cases identified through tracing of contacts improved from 6% to 10% while the percentage of notified TB cases; all forms contributed through community referrals improved from 4% to 8%. Community health volunteers can play an effective role in household contact screening and referrals for identification of TB.
Prevalence of reduced visual acuity among school-aged children and adolescents in 6 districts of Changsha city: a population-based survey
Authors: Liao Menglian , Cai Zehuai , Khan Muhammad Ahmad , Miao Wenjie , Lin Ding , Tang Qiongyan
Published: January 2020

Background: To calculate and evaluate the prevalence of reduced visual acuity(VA) in primary and secondary school students in 6 districts of Changsha, Hunan, China. Methods: A population-based retrospective study was conducted in 239 schools in 6 districts of Changsha. After routine eye examination to rule out diseases that can affect refraction, 250,980 eligible students from elementary, middle and high schools were enrolled in the survey. Then the uncorrected distant and near visual acuity of each eye were measured. Categories of key schools, districts, grades, eye exercises and sports time were also documented and analyzed. Results: The overall prevalence of reduced VA was 51.8% (95% confidence interval [CI]: 51.6%-52.0%) in 6 districts of Changsha. Results of individual districts were as follows: Furong district 59.9%(95% CI: 57.9%-61.8%), Tianxin district 62.3%(95% CI: 60.5%-64.0%), Wangcheng district 47.8%(95% CI: 46.8%-48.8%), Kaifu district 58.5%(95% CI: 58.0%-58.9%), Yuhua district 47.0%(95% CI: 46.7%-47.4%) and Yuelu district 52.6%(95% CI: 52.3%-52.9%). The proportion of normal VA is seen to decrease from primary grade 3. The proportion of mildly reduced VA is higher in primary grade 1 and 2. The proportion of moderately reduced VA remains similar during 12 grades. The proportion of severely reduced VA increases with grades. Multivariate analysis shows that the prevalence of reduced VA is lower in the province level key schools (odds ratio [OR]=1.23, 95% CI 1.13-1.33) than in the municipal level key schools (OR=1.47, 95% CI 1.44-1.50). Conclusion: According to the existing data analysis results, the prevalence of reduced VA among primary and secondary school students in Changsha is very high. Some effective measures need to be taken to prevent it.
Predictive model for early diagnosis of keratoconus
Authors: CASTRO-LUNA GRACIA , PÉREZ-RUEDA ANTONIO
Published: January 2020

Background To describe the topographic, pachymetric and aberrometry characteristics in patients with keratoconus, subclinical keratoconus/forme fruste and normal corneas. Calculate a diagnostic model of subclinical keratoconus/forme fruste. Methods The design was a cross-sectional study. It included 205 eyes from 188 patients distributed in 82 normal corneas, 40 subclinical keratoconus/forme fruste and 83 established keratoconus The topographic, pachymetric and aberrometry variables obtained by rotary Scheimpflug camera (Pentacam® type) were analyzed. A descriptive and bivariate analysis of the recorded data was performed. A diagnostic model of subclinical keratoconus/forme fruste was calculated. Results Statistically significant differences were obtained when comparing normal corneas with subclinical keratoconus/forme fruste in variables of vertical asymmetry and corneal thickness.The regression model was calculated with the minimum corneal thickness and the anterior coma to 90º and posterior coma to 90º. Conclusions The diagnosis of subclinical keratoconus/forme fruste depends on the central corneal thickness, and two aberrometric topographic parameters the anterior coma to 90º and posterior coma to 90º.
Adults´ dental treatment in 2001-2013 in Finnish Public Dental Service
Authors: Linden Jari , Widström Eeva , Sinkkonen Janne
Published: January 2020

Background All adults over 17 years of age have access to the Public Dental Service after the Finnish Dental Care Reform in 2001-2002. This study aimed to survey the treatment needs and treatment measures provided for adult patients and changes in these during the period 2001–2013.Methods Using each person’s unique identifier, demographic data on dental visits during the period 2001 - 2013 were collected from municipal databases in five PDS-units covering 320,000 inhabitants. The numbers of visitors, those in need of basic periodontal or caries treatment (CPI >2 and D + d > 0) were calculated for three age groups. Treatment provided was also calculated in 13 treatment categories. Trend analyses were performed to study changes during the study period.Results Restorative treatments (968,772; 23.6%), examinations (658,394; 16.1%), radiographs taken (529,875; 12.9%) anaesthesia used (521,169; 12.7%) and emergency treatments (348,229; 8.5%) made up 73.8% of all treatment measures during the entire study period. Periodontal treatment (7.8%) and caries prevention (3.9%) made up a small part of the care provided and prosthetics and treatment of TMJ disorders were extremely uncommon (fewer than 1%). Treatments related to caries (restorative treatment, examinations, endodontics, emergencies, anaesthesia and radiographs) made up 60.4% of the dental personnel’s treatment time. During the study period, statistically significant increasing trends were found for radiographs (p<0.001***), anaesthesia (p=0.003**) and total number of treatments (p=0.009**). There was a slight decreasing trend in treatment need among the youngest adults (18-39years; p=0.033*).Conclusion Compared with the results of national epidemiological studies, insufficient periodontal treatment is provided and prosthetic treatment is almost totally neglected in the PDS. Rather, adults’ dental treatment concentrates on treatment of caries. The unmet needs may be due to tradition, inadequate treatment processes or a lack of resources or failed salary incentives.
Chemosensory Dysfunction and Oral Disorders Affect Oral Health-Related Quality Of Life in Patients with Primary Sjögren's Syndrome
Authors: Gobeljić Mirjana Šijan , Milić Vera , Pejnovic Nada , Damjanov Nemanja
Published: January 2020

Background: The aim of this study was to evaluate chemosensory function and oral disorders in patients with primary Sjögren's syndrome (pSS) and to compare these findings with those of age- and gender-matched healthy controls.Methods: Olfactory and gustatory function, dysgeusia, burning sensations in the tongue (BST), halitosis, and oral health-related quality of life (OHRQoL) using the short-form Oral Health Impact Profile (OHIP-14) were evaluated in 58 patients with primary Sjögren’s syndrome (pSS) and 55 age- and gender-matched healthy controls.Results: Patients with pSS had significantly lower self-reported visual analogue scale (VAS) smell score (8.6±2.2 vs. 9.6±0.7, p=0.016) and VAS taste score (9.5±0.7 vs. 8.5±2.1, p=0.014) than healthy controls. A greater proportion of patients with pSS had anosmia (3.8% vs. 0.0%) or hyposmia (36.5% vs. 13.2%) and ageusia for basic tastes: sweetness (34.0% vs. 7.5%), sourness (10.6 % vs. 0.0), saltiness (10.0% vs. 5.7%) or bitterness (19.1% vs. 1.9%). A higher proportion of pSS patients complained of dysgeusia (52.6% vs. 9.4%, p<0.0001) and BST (45.6% vs. 0.0%, p<0.0001) while similar number of pSS patients and controls reported halitosis (31.6% vs. 28.3%, p=0.434). The mean OHIP-14 score was significantly higher in patients with pSS (6.8±7.0 vs. 2.3±8.5, p<0.001) indicating patients’ poorer OHRQoL compared to controls. Conclusions: The majority of patients with pSS had impaired chemosensory function and indicators of oral health in comparison to the age- and gender-matched healthy controls. Further studies of oral hygiene habits and dietary intake of these patients are needed to ensure better management of oral health problems in patients with pSS.
Molecular analysis of lipid uptake- and necroptosis-associated factor expression in vitrified-warmed mouse oocytes
Authors: Um Da-Eun , Shin Hyejin , Park Dayoung , Ahn Jeong Min , Kim Jayeon , Song Haengseok , Lim Hyunjung Jade
Published: January 2020

Background: We had previously demonstrated that vitrification reduces the levels of certain phospholipid classes, and that oocytes from aged mice show a similar lipidome alteration, even without vitrification. In the current investigation, we examined if vitrification-warming of mouse oocytes from young and aged mice causes any changes in molecular aspects of lipid-associated features. Methods: Metaphase II (MII) stage oocytes were harvested from young (10‒14-week-old) and aged (45‒54-week-old) mice by a superovulation regime with PMSG followed by hCG. We examined the status of the intracellular lipid pool and the integrity of the plasma membrane by staining oocytes with BODIPY 500/510 and CellMask live dyes. Expression of lipid uptake- and necroptosis-associated genes was assessed by quantitative PCR analyses, in oocytes from young and old mice, before and after vitrification. Localization patterns of two crucial necroptosis proteins, phosphorylated MLKL (pMLKL) and phosphorylated RIPK1 (pRIPK1) were examined in mouse oocytes by immunofluorescence staining. Necrostain-1 (Nec1), an inhibitor of RIPK1, was used to examine if RIPK1 activity is required to maintain oocyte quality during vitrification. Results: We confirmed that vitrified-warmed oocytes from aged mice showed noticeable decrease in both CellMask and BODIPY 500/510 dyes. Among the lipid uptake-associated genes, Cd36 expression was higher in oocytes from aged mice. Necroptosis is a type of programmed cell death that involves damage to the plasma membrane, eventually resulting in cell rupture. The expression of necroptosis-associated genes did not significantly differ among groups. We observed that localization patterns of pMLKL and pRIPK1 were unique in mouse oocytes, showing association with microtubule organizing centers (MTOCs) and spindle poles. pMLKL was also localized on kinetochores of MII chromosomes. Oocytes treated with Nec1 during vitrification showed a decreased survival rate, indicating the importance of RIPK1 activity in oocyte vitrification.Conclusions: We report that oocytes from aged mice show differential expression of CD36, which suggests that CD36-mediated lipid uptake may be influenced by age. We also show for the first time that pMLKL and pRIPK1 exhibit unique localization pattern in mouse oocytes and this may suggest role(s) for these factors in non-necroptosis-associated cellular processes.
Outcomes following administration of tranexamic acid in combat-related intracranial hemorrhage: a cohort study
Authors: Walker Patrick F , Bozzay Joseph D. , Johnston Luke R. , Elster Eric A. , Rodriguez Carlos J. , Bradley Matthew J.
Published: January 2020

BACKGROUND: Tranexamic acid (TXA) may be a useful adjunct for military patients with severe traumatic brain injury (TBI). These patients are often treated in austere settings without immediate access to neurosurgical intervention. The purpose of this study was to evaluate any association between TXA use and progression of intracranial hemorrhage (ICH), neurologic outcomes, and venous thromboembolism (VTE) in TBI.METHODS: This was a retrospective cohort study of combat casualties from October 2010 to December 2015 who were transferred to a military treatment facility (MTF) in the United States. Data collected included: demographics, types of injuries, initial and interval head computerized tomography (CT) scans, Glasgow Coma Scores (GCS), and six-month Glasgow Outcome Scores (GOS). Results were stratified based on TXA administration, progression of ICH, and VTE.RESULTS: Of the 687 active duty service members reviewed, 71 patients had ICH (10.3%). Most casualties were injured in a blast (80.3%), with 36 patients (50.7%) sustaining a penetrating TBI. Mean ISS was 28.2 ± 12.3. Nine patients (12.7%) received a massive transfusion within 24 hours of injury, and TXA was administered to 14 (19.7%) casualties. Patients that received TXA had lower initial reported GCS (9.2 ± 4.4 vs. 12.5 ± 3.4, p=0.003), similar discharge GCS (13.3 ±4.0 vs. 13.8±3.2, p=0.58), and a larger improvement between initial and discharge GCS (3.7±3.9 vs. 1.3±3.1, p=0.02). However, there was no difference in mortality (7.1% vs. 7.0%, p=1.00), progression of ICH (45.5% vs. 14.7%, p=0.09), frequency of cranial decompression (50.0% vs. 42.1%, p=0.76), or mean GOS (3.5±0.9 vs. 3.8±1.0, p=0.13). Patients administered TXA had a higher rate of VTE (35.7% vs. 7.0%, p=0.01). On multivariate analysis, however, TXA was not independently associated with VTE.CONCLUSIONS: Patients that received TXA were associated with an improvement in GCS but not in progression of ICH or GOS. TXA was not independently associated with VTE, although this may be related to a paucity of patients receiving TXA. Decisions about TXA administration in combat casualties with ICH should be considered in the context of the availability of neurosurgical intervention as well as severity of extracranial injuries and need for massive transfusion.
Potential mechanisms of quantitative resistance to Leptosphaeria maculans (blackleg) on cotyledons of canola (Brassica napus)
Authors: Hubbard Michelle , Zhai Chun , Peng Gary
Published: January 2020

Background: Blackleg disease, caused by Leptosphaeria maculans (Lm), can lead to significant losses of canola/rapeseed crops. Growing resistant canola cultivars can be an effective and environmentally friendly way to manage blackleg. Major resistance genes may stop infection, but can also be rapidly overcome by shifts in pathogen population towards virulence. Thus, using race-nonspecific or quantitative resistance (QR) is of interest because it is potentially more durable. However, the mechanisms and genes underlying QR are mostly unknown. In this study, we explored QR in “74‑44 BL”, a Canadian canola cultivar carrying a moderate level of race nonspecific resistance, based on cotyledon inoculation (Supple. Fig.1) . The susceptible cultivar “Westar” was used as a control. Lesions developed more slowly on 74-44BL than on Westar. We used RNA-Seq to identify genes and gene functions putatively involved in the QR. Results: Relative to inoculated Westar, some of the B. napus genes that were differentially expressed strongly in inoculated 74-44 BL included those putatively involved in programmed cell death (PCD), reactive oxygen species (ROS) generation, signal transduction and/or intracellular endomembrane transport. Examples included genes annotated as a Bax inhibitor 1, a development/cell death (DCD) domain containing proteinases and peptidases, all of which could play a role in PCD and a zinc-finger Sec23/Sec24 and five small GTPases likely involved in endoplasmic reticulum (ER) to Golgi vesicle traffic and/or signal transduction. Further experiments, however, did not confirm changes in genomic DNA degradation, a potential marker for PCD, between the two cultivars. In addition, infection progression in cotyledons was not altered by applying protease inhibitors directly to cotyledons. Additional testing was done using green fluorescent protein (GFP)-tagged Lm for cotyledon colonization as well as ROS production, in relation to the lesion development. The results showed that ROS production occurred beyond the area colonized by Lm hyphae in 74-44 BL.Conclusions: ROS may also be involved in signal transduction and/or intracellular endomembrane transport. These results provide a starting point for a better understanding of the mechanisms behind QR against Lm in canola and developing new host-resistance strategies for management of blackleg.
No Negative Influence On Complications of femoral neck fracture Children Age Between 14 And 17 Years Treatment with Cannulated Screws Transphyseal fixation
Authors: Liu Yang , Zhao Xiao-Dan
Published: January 2020

Background Hip fractures represent less than 1% of pediatric fractures. The physeal of femoral head closure at 14 to 17 years of age, and it provides capacity for spontaneous fracture remodeling. The primary purpose of the current retrospective study was to determine the influence of transphyseal fixation of cancellous screws on the outcomes in this age group. Methods: From April 2007 to August 2016, all patients age between 14 to 17 years with Delbet type II and Type III hip fracture treatment with cancellous screws fixation were included. According to whether the cancellous screws thread across the proximal femur epiphysis, we divided the patients into the no cross epiphysis (NCE) group and cross epiphysis (CE) group. The outcome was analysis regarding osteonecrosis of femoral head, nonunion, delay union, premature physeal closure (PPC), coxa vara and overgrowth of femoral neck. Results: Of the 28 patients were included in the study, most patients were male (92.9%).19 were classified as Delbet type II, 9 were type III. The CE and the NCE group both included 14 patients. The average follow-up time is 37.8 months (range 26-68 months). The rate of superficial infection, deep infection, AVN, non-union, delay union, coxa vara, PCE, overgrowth was not significantly different between the two groups. Conclusion: This study demonstrated that the transphyseal fixation of cannulated screws has no negative influence on the outcomes, especially on femoral head osteonecrosis. Achievement of accurate reduction and rigid fixation for this unique injury is more important than preservation of the proximal femoral epiphysis.
Schizophyllum commune induced oxidative stress and immunosuppressive activity in Spodoptera litura
Authors: Kaur Mandeep , Chadha Pooja , Kaur Sanehdeep , Kaur Amarjeet , Kaur Rajvir
Published: January 2020

Background: In a search for the mechanism of action of an endophytic fungus Schizophyllum commune against Spodoptera litura, the effect of its ethyl acetate extract was judged on antioxidant and detoxifying enzymes as well as on morphology of haemocytes. Results: Ethyl acetate extract of S.commune was administrated to the larvae of S.litura using artificial diet method having concentration 276.54µg/ml (LC50 of fungus). The effect on antioxidant and detoxifying enzymes (Catalase, Ascorbate peroxidase, Superoxide dismutase, Glutathione-S-Transferase) was observed using haemolymph and midgut of insect larvae for different time intervals (24, 48, 72 and 96). In additionally, haemocytes morphology was also studied using Scanning Electron Microscopy (SEM) after 96hr. In particular, to resist the toxicity, the activities of the antioxidant and detoxifying enzymes (Catalase, Ascorbate peroxidase, Superoxide dismutase, Glutathione-S-Transferase) significantly (p≤0.05) increased in both the tissues of treated larvae as compared to control. The exposed group revealed various deformities in haemocytes of S.litura like breakage in the cell membrane, cytoplasmic leakage and appearance of strumae etc. Conclusion: This study provides important information regarding the oxidative stress causing potential and immunosuppressant nature of S.commune against S.litura and elucidates the patterns of antioxidant and detoxifying enzymes activities and changes in haemocytes in the susceptible larvae.
Measuring quality of life and identifying what is important to Jordanian living with Multiple Sclerosis using the Arabic version of the Patient Generated Index (PGI)
Authors: Aburub Ala' S. , Khalil Hanan , Al-Sharman Alham , El-Salem Khalid
Published: January 2020

Background Patients Generated index (PGI) is one of the individualized measures used to measure the quality of life (QOL) in people with different chronic conditions including multiple sclerosis (MS). However, the psychometric properties of the Arabic version of the PGI have not been fully established in Jordanian living with MS. Therefore, the objective of this study is to identify what matters to Jordanian living with MS and to contribute evidence toward the psychometric properties of the Arabic version of the PGI. Method A total of 75 participants with MS completed three QOL measures; PGI, the Patient Determined Disease Steps (PDDS), and EQ-5D. Generalized Estimating Equations (GEE) were used to compare the total score of three QOL measures. Bland-Altman plot and Spearman’s correlation coefficient were used to study the relationships and differences between the PGI and the other study measures (PDDS and EQ-5D). Results Only 66 (88%) of the participants were able to complete the PGI. Overall, 36 areas of QOL concern were nominated by the participants using the PGI with the top 3 areas were an emotional function (47%), involuntary movement reaction functions (45.5%) and walking (44%). The average global score of the PGI was lower (34±22) than the global score of the EQ-5D (69±23) and the PDDS (68±24).PGI had a moderate correlation with EQ-5D and PPDS. Conclusion The Arabic version of the PGI is a feasible and acceptable measure among Jordanian with MS and it captures a wide spectrum of important areas that contribute to QOL than EQ-5D and PDDS. PGI could improve the decision making and guide health-care professionals to provide appropriate intervention programs to reduce the burdens from MS disease and improve QOL.
Association between pH and neurological outcome among cardiac arrest patients treated by extracorporeal CPR: a prospective observational cohort study in Japan
Authors: OKADA YOHEI , Kiguchi Takeyuki , Irisawa Taro , Yoshiya Kazuhisa , Yamada Tomoki , Hayakawa Koichi , Noguchi Kazuo , Nishimura Tetsuro , Ishibe Takuya , Yagi Yoshiki , Kishimoto Masafumi , Shintani Hiroshi , Hayashi Yasuyuki , Sogabe Taku , Morooka Takaya , Sakamoto Haruko , Suzuki Keitaro , Nakamura Fumiko , Nishioka Norihiro , Matsuyama Tasuku , Sado Junya , Matsui Satoshi , Shimazu Takeshi , Koike Kaoru , Kawamura Takashi , Kitamura Tetsuhisa , Iwami Taku
Published: January 2020

Background We aimed to identify the association of pH value in blood gas assessment with neurological outcome among out-of-hospital cardiac arrest (OHCA) patients treated by extracorporeal cardiopulmonary resuscitation (ECPR). Methods We retrospectively analysed the database of a multicentre prospective observational study to collect pre-hospital and in-hospital data among OHCA patients in Osaka prefecture, Japan (CRITICAL study), from 1 July 2012 to 31 December 2016. We included the adult OHCA patients treated by ECPR with blood gas assessment before starting ECPR. Patients with OHCA of external causes, such as trauma, were excluded. The patients were divided into three approximately equal groups based on the pH values. We conducted logistic regression analysis to identify the odds ratio (OR) and 95% confidence interval (CI) of the groups for the 1-month neurological favourable outcome adjusted by the potential confounders of sex, age, witnessed by bystander, CPR by bystander, pre-hospital initial cardiac rhythm, and cardiac rhythm on hospital arrival. Results Among the 9,822 patients in the database, 260 patients were finally included in the analysis. The three groups were Tertile 1: pH ≥7.030, Tertile 2: pH 6.875-7.029, and Tertile 3: pH<6.875. The adjusted OR of Tertiles 2 and 3 compared with Tertile 1 for 1-month favourable neurological outcome were 0.26 (95% CI: 0.10-0.63) and 0.24 (95% CI: 0.09-0.61), respectively. Conclusion This multi-institutional observational study showed that low pH value (<7.03) before the implementation of ECPR was associated with 1-month unfavourable neurological outcome among OHCA patients treated with ECPR. It may be helpful to consider the candidate for ECPR.
Validity assessment of self-reported medication use for hypertension, diabetes and dyslipidemia in a pharmacoepidemiologic study by comparison with health insurance claims
Authors: Matsumoto Minako , Harada Sei , Iida Miho , Kato Suzuka , Sata Mizuki , Hirata Aya , Kuwabara Kazuyo , Takeuchi Ayano , Sugiyama Daisuke , Okamura Tomonori , Takebayashi Toru
Published: January 2020

Background Although self-reported questionnaires are widely employed in epidemiologic studies, their validity has not been sufficiently assessed. The aim of this study was to evaluate the validity of a self-reported questionnaire on medication use by comparison with health insurance claims and to identify individual determinants of discordance.Methods Participants were 2,720 community-dwellers aged 37 to 78 years from the Tsuruoka Metabolomics Cohort Study. Information on lifestyle and medications was collected through a questionnaire. Sensitivity and specificity were determined using health insurance claims from November 2014 to March 2016, which were used as a standard. Potential determinants of discordance were assessed by multivariable logistic regression.Results The self-reported questionnaire on medication use showed high validity. Sensitivity and specificity were 0.95 (95% CI:0.93-0.96) and 0.94 (0.93-0.95) for antihypertensives, 0.94 (0.91-0.97) and 0.98 (0.98-0.99) for diabetes medications, 0.84 (0.82-0.87) and 0.96 (0.95-0.97) for dyslipidemia medications, respectively. Males without high education were found to be associated with discordant reporting, especially those with medication use for dyslipidemia.Conclusions In this population-based study, we found that the self-reported questionnaire on medication use was a valid measure of true medication use. Sensitivity for dyslipidemia medications was lower than those for the other medications. Type of medication, gender and education influenced discordance in self-reporting.
RNA-seq analysis of galaninergic neurons from ventrolateral preoptic nucleus identifies expression changes between sleep and wake
Authors: Guo Xiaofeng , Gao Xiaoling , Keenan Brendan T. , Zhu Jingxu , Sarantopoulou Dimitra , Lian Jie , Galante Raymond J. , Grant Gregory R. , Pack Allan I.
Published: January 2020

Background : Previous studies show that galanin neurons in ventrolateral preoptic nucleus (VLPO-Gal) are essential for sleep regulation. Here, we explored the function of the VLPO-Gal neurons in sleep by comparing their transcriptional responses between sleeping mice and those kept awake, sacrificed at the same diurnal time. Results : RNA-sequencing (RNA-seq) analysis was performed on eGFP(+) galanin neurons isolated using laser captured microdissection (LCM) from VLPO. Expression of Gal was assessed in our LCM eGFP(+) neurons via real time qPCR and showed marked enrichment when compared to LCM eGFP(-) neurons and to bulk VLPO samples. Gene set analysis utilizing data from a recent single-cell RNA-seq study of the preoptic area demonstrated that our VLPO-Gal samples were highly enriched with galanin-expressing inhibitory neurons, but not galanin-expressing excitatory neurons. A total of 263 genes were differentially expressed between sleep and wake in VLPO-Gal neurons. When comparing differentially expressed genes in VLPO-Gal neurons to differentially expressed genes in a wake-active neuronal region (the medial prefrontal cortex), evidence indicates that both systemic and cell-specific mechanisms contribute to the transcriptional regulation in VLPO-Gal neurons. In both wake-active and sleep-active neurons, ER stress pathways are activated by wake and cold-inducible RNA-binding proteins are activated by sleep. In contrast, expression of DNA repair genes is increased in VLPO-Gal during wakefulness, but increased in wake-active cells during sleep. Conclusion : Our study identified transcriptomic responses of the galanin neurons in the ventrolateral preoptic nucleus (VLPO) during sleep and sleep deprivation. Data indicate that VLPO contains mainly sleep-active inhibitory galaninergic neurons. The VLPO galanin neurons show responses to sleep and wake similar to wake-active regions, indicating these responses, such as ER stress and cold-inducible RNA-binding proteins, are systemic affecting all neuronal populations. Region-specific differences in sleep/wake responses were also identified, in particular DNA repair, suggesting these could be driven by neuronal activity. Our study expands knowledge about the transcriptional response of a distinct group of neurons essential for sleep.
“Everything is provided free, but they are still hesitant to access healthcare services”: Why does the Indigenous community in Attapadi, Kerala continue to experience poor access to healthcare?
Authors: George Mathew Sunil , Davey Rachel , Mohanty Itismita , Upton Penney
Published: January 2020

Background Inequity in access to healthcare services is a constant concern. While advances in healthcare have progressed in the last several decades, thereby significantly improving the prevention and treatment of disease, these benefits have not been shared equally. Excluded communities such as Indigenous communities typically face a lack of access to healthcare services that others do not. This study seeks to understand why the indigenous community in Attapadi continues to experience poor access to healthcare in spite of both financial protection and adequate coverage of health services Methods Ethnographic fieldwork was carried out among the various stakeholders living in Attapadi . A total of 52 in-depth interviews and 5 focus group discussions were conducted amongst the indigenous community, the healthcare providers and key informants. The data was coded utilising a reflexive and inductive approach leading to the development of the key categories and themes Results The health system provided a comprehensive financial protection package in addition to a host of healthcare facilities for the indigenous community to avail services. In spite of this, the community resisted the attempts of the health system to improve their access. The failure to provide culturally respectful care, the discrimination of the community at healthcare facilities, the centralisation of the delivery of services as well as the lack of power among the indigenous community to negotiate with the health system for services that were less disruptive to their lives were barriers to improving healthcare access. Power differentials between the community and the health system stakeholders also ensured that meaningful involvement of the community in the local health system did not occur. Conclusion Improving access to health care for indigenous communities would require UHC interventions to be culturally safe, locally relevant and promote active involvement of the community at all stages of the intervention. Structural power imbalances that affect access to resources and prevent meaningful involvement of indigenous communities also need to be addressed.
The relational development of infants with CLP: impact on parents and on the parent-infant relationship. Results from the first French prospective multicenter CLP study.
Authors: GROLLEMUND BRUNO , Dissaux Caroline , Gavelle Pascale , Martinez Carla Perez , Mullaert Jimmy , Alfaiate Toni , Guedeney Antoine
Published: January 2020

Background : The objective of this prospective, multidisciplinary and multicenter study was to explore the impact of a cleft lip, whether or not in association with a cleft palate (CLP) on the parents, on the parent-infant relationship, and on the infant’s relational development, depending on the type of malformation and the timing of the repair surgery, at 4 and 12 months postpartum. Method : 158 infants with CLP and their parents participated in this multicenter prospective cohort. Clinical evaluations were performed at 4 and 12 months postpartum. The impact on the parents and on the parent-infant relationship was evaluated by the Parenting Stress Index (PSI), the Edinburgh Post-partum Depression Scale (EPDS) and the Impact-on-Family Scale (IOFS). The relational development of the infant was assessed using the Alarm Distress Baby Scale (ADBB). The main criteria used to compare the infants were the severity of CLP and the time of surgery. Results 1) No effect was found in this sample of the timing of surgery, the type of malformation or the care structure on social withdrawal behaviors of the child at 4 and 12 months postpartum (ADBB). Furthermore, early intervention significantly decreased maternal stress assessed with the PSI at 4 months. 2) Parents for whom it had been possible to give a prenatal diagnosis were much better prepared to accept the waiting time between birth and the first surgical intervention (PSI, EPDS, IOFS). 3) Higher postpartum depression scores (EPDS) were found for both parents compared to the general population. Conclusion : A joint assessment of the mental health of both infants and parents is required in the follow-up of cleft lip and palate. Even if most families are remarkably resilient when faced with this major cause of stress, a significant proportion of them could require help to deal with the situation, especially during this first year of follow-up. An assessment of social withdrawal behaviours in the child and of the level of parental stress and depression appears useful, in order to adapt care to infant and parents alike. Trial Registration: ClinicalTrials.gov Identifier: NCT00993993. Registered 10/14/2009, https://clinicaltrials.gov/ct2/show/NCT00993993?term=grollemund&draw=2&rank=1.
Better efficacy in differentiating WHO grade II from III oligodendrogliomas with machine-learning than radiologist’s reading from conventional T1 contrast-enhanced and fluid attenuated inversion recovery images
Authors: Zhao Sha-Sha , Feng Xiu-Long , Hu Yu-Chuan , Han Yu , Tian Qiang , Sun Ying-Zhi , Zhang Jie , Ge Xiang-Wei , Cheng Si-Chao , Li Xiu-Li , Mao Li , Shen Shu-Ning , Yan Lin-Feng , Cui Guang-Bin , Wang Wen
Published: January 2020

Background: The medical imaging to differentiate World Health Organization (WHO) grade II (ODG2) from III (ODG3) oligodendrogliomas still remains a challenge. We investigated whether combination of machine leaning with radiomics from conventional T1 contrast-enhanced (T1CE) and fluid attenuated inversion recovery (FLAIR) magnetic resonance imaging (MRI) offered superior efficacy. Methods: Thirty-six patients with histologically confirmed ODGs underwent T1CE and 33 of them underwent FLAIR MR examination before any intervention from January 2015 to July 2017 were retrospectively recruited in the current study. The volume of interest (VOI) covering the whole tumor enhancement were manually drawn on the T1CE and FLAIR slice by slice using ITK-SNAP and a total of 1072 features were extracted from the VOI using 3-D slicer software. Random forest (RF) algorithm was applied to differentiate ODG2 from ODG3 and the efficacy was tested with 5-fold cross validation. The diagnostic efficacy of radiomics-based machine learning and radiologist’s assessment were also compared. Results: Nineteen ODG2 and 17 ODG3 were included in this study and ODG3 tended to present with prominent necrosis and nodular/ring-like enhancement (P < 0.05). The AUC, ACC, sensitivity, and specificity of radiomics were 0.798, 0.735, 0.672, 0.789 for T1CE, 0.774, 0.689, 0.700, 0.683 for FLAIR, as well as 0.861, 0.781, 0.778, 0.783 for the combination, respectively. The AUCs of radiologists 1, 2 and 3 were 0.700, 0.687, and 0.714, respectively. The efficacy of machine learning based on radiomics was superior to the radiologists' assessment. Conclusions: Machine-learning based on radiomics of T1CE and FLAIR offered superior efficacy to that of radiologists in differentiating ODG2 from ODG3.
Biochar Affects Essential Nutrients of Carrot Taproots and Lettuce Leaves
Authors: Olszyk David M. , Shiroyama Tamotsu , Novak Jeffrey M. , Cantrell Keri B. , Sigua Gilbert , Watts Donald W. , Johnson Mark G.
Published: January 2020

Essential nutrient concentrations in crops can affect human health. While biochar has the potential as a soil amendment to improve crop yields, it may also affect the concentrations of nutrients such as Ca, Fe, K, Mg, Mn, and Zn in edible portions of crops. To better characterize effects of biochar on important human nutrients in food crops, we evaluated the effects of biochar on lettuce (Lactuca sativa L. cv. Black-Seeded Simpson) leaf and carrot [Daucus carota subsp. sativus (Hoffm.) Schübl. cv. Tendersweet] developing taproot nutrients. Plants were grown in pots in a greenhouse using sandy loam (Coxville, fine, kaolinitic, thermic Typic Paleaquults) and loamy sand (Norfolk, fine-loamy, kaolinitic, thermic Typic Kandiudults,) series soils, amended with biochar produced from four feedstocks: pine chips (PC), poultry litter (PL), swine solids (SS), and switchgrass (SG); and two blends of PC plus PL [PC/PL, 50%/50% (55) and 80%/20% (82) by weight]. Biochar was produced at 350, 500, and 700 °C from each feedstock. Lettuce leaf and carrot taproot total nutrient concentrations were determined by inductively coupled plasma analysis. Biochar (especially at least in part manure-based, i.e., PL, SS, 55, and 82 at nearly all temperatures) primarily decreased nutrient concentrations in lettuce leaves, with Ca, Mg, and Zn affected most. Carrot taproot nutrient concentrations also deceased, but to a lesser extent. Some biochars increased leaf or taproot nutrient concentrations, especially K. This study indicated that biochar can both decrease and increase leaf and taproot nutrient concentrations important for human health. Thus, potential effects on nutrients in plants should be carefully considered when biochar is used as a soil amendment with vegetable crops.
Evaluation of Several Reference Evapotranspiration Models and Determination of Crop Water Requirement for Tomato in a Solar Greenhouse
Authors: Gong Xuewen , Wang Shunsheng , Xu Cundong , Zhang Hao , Ge Jiankun
Published: January 2020

Studies on dual crop coefficient method in a greenhouse require accurate values of reference evapotranspiration (ETo). This study was conducted in a solar greenhouse at the experimental station of the Chinese Academy of Agricultural Sciences during 2015 and 2016. An automatic weather station was installed in the center of the same greenhouse to record weather parameters at 30-minute intervals. Five ETo models (Penman-Monteith, Penman, radiation, pan evaporation, and Priestley-Taylor) were employed, and their performance was evaluated using the dual crop coefficient method. The basal crop coefficient Kcb and soil evaporation coefficient Ke were adjusted according to the surrounding climate inside the greenhouse. Crop evapotranspiration (ETc) was continuously measured using sap flow system combined with microlysimeter in 2015 and weighing lysimeters in 2016. Daily ETo was simulated from the five models and compared with the measurements. Results show that the adjusted Kcb values were 0.15, 0.94, and 0.65 in 2015 and 0.15, 1.02, and 0.70 in 2016 at initial, midseason, and late-season, respectively. The Ke varies between 0.10 and 0.45 during the whole growth period. The ETc was ≈345 mm for drip-irrigated tomato in solar greenhouse at the whole growth stage. The radiation and pan evaporation models tend to overestimate ETo values. Results of the Penman-Monteith, Penman, and Priestley-Taylor models show comparatively good performance in estimating ETo. Considering the robustness and simplicity, the Priestley-Taylor was recommended as the first choice to estimate ETo of tomato grown in a solar greenhouse. This work can help farmers to optimize the irrigation scheduling based on an ETo model for solar greenhouse vegetables in northern China.
Expression Analysis of ERFs during Storage under Modified Atmosphere Packaging (High-concentration of CO2) of Fresh-cut Lotus Root
Authors: Liu En-chao , Niu Li-fang , Yi Yang , Wang Li-mei , Ai You-wei , Zhao Yun , Wang Hong-xun , Min Ting
Published: January 2020

Ethylene response factor (ERF) genes have been characterized in numerous plants, where they are associated with responses to biotic and abiotic stress. Modified atmosphere packaging (MAP) is an effective treatment to prevent lotus root browning. However, the possible relationship between ERF transcription factors and lotus root browning under MAP remains unexplored. In this study, the effects of phenol, phenylalanine ammonia lyase (PAL), polyphenol oxidase (PPO), and peroxidase (POD) enzyme activities; and PPO, PAL, POD, and ERF gene expression on fresh-cut lotus root browning were studied with MAP. The expression pattern of ERF2/5 correlated highly with the degree of browning. It is suggested that NnERF2/5 can be used as an important candidate gene for the regulation of fresh-cut lotus root browning under MAP, and the correlation of each gene should be studied further.
Using Text Mining to Gauge Student Sentiments about Hands-on Activities in a Protected Agriculture Course
Authors: Nunez Gerardo H.
Published: January 2020

Hands-on activities enhance learning and increase student satisfaction in horticulture courses. Nevertheless, hands-on activities can have widely different impacts on student learning. To achieve and maintain educational quality, instructors need to evaluate and improve activities. This research used text mining and sentiment analysis to gauge student sentiments about hands-on activities in a protected agriculture course. Students participated in five hands-on activities and submitted short reflective essays about them. Essay texts were separated into single-word (unigram) or three-word (trigram) objects. Unigrams were compared with general-use emotion lexica to extract student sentiments from the texts. Trigrams were used to assess essay content. All activities elicited positive sentiments among students. Trust, anticipation, and joy were the most prominent emotions identified. The activity focused on freeze protection was preferred over the other activities. Although other activities were also well received, they should be refined for future offerings. The presented method could be used to assess hands-on activities, leading to continuous improvement and successful implementation of experiential learning in horticulture courses.
Financial Development and the Effectiveness of Monetary Policy: New Evidence from Malaysia
Authors: Basa Danie Eirieswanty Kamal , Abdul Karim Zulkefly , Zaidi Mohd Azlan Shah
Published: January 2020

This paper examines the relationship between financial development (FD) and the monetary policy effectiveness (MPE) in Malaysia. Two indicators designate the level of FD, namely liquid liabilities and stock market capitalisation, and gross fixed capital formation are used in estimating the determinants of MPE which is generated using structural vector auto regressive (SVAR) in an open economy approach. Accordingly, an autoregressive distributed lag (ARDL) model is used in examining the effects of FD and gross fixed capital formation on MPE. Using a yearly time series data spanning from 1991 to 2016, the results revealed that there is a cointegration between FD and gross fixed capital formation on MPE. Interestingly, FD has a negatively significant effect on MPE on output and inflation, which indicates that a more developed financial system has hindered the effectiveness of the monetary policy.