bluuebunny/crossref_metadata_2025 huggingface dataset:
In Silico Analysis of Bioactive Peptides Released from Giant Grouper (Epinephelus lanceolatus) Roe Proteins Identified by Proteomics Approach
Authors: Panjaitan Fenny Crista A. , Gomez Honey Lyn R. , Chang Yu-Wei
Published: November 2018

Major proteins contained in dried giant grouper roe (GR) such as vitellogenin (from Epinephelus coioides; NCBI accession number: AAW29031.1), apolipoprotein A-1 precursor (from Epinephelus coioides; NCBI accession number: ACI01807.1) and apolipoprotein E (from Epinephelus bruneus; NCBI accession number: AEB31283.1) were characterized through compiled proteomics techniques (SDS-PAGE, in-gel digestion, mass spectrometry and on-line Mascot database analysis). These proteins were subjected to in silico analysis using BLAST and BIOPEP-UWM database. Sequence similarity search by BLAST revealed that the aligned vitellogenin sequences from Epinephelus coioides and Epinephelus lanceolatus share 70% identity, which indicates that the sequence sample has significant similarity with proteins in sequence databases. Moreover, prediction of potential bioactivities through BIOPEP-UWM database resulted in high numbers of peptides predominantly with dipeptidyl peptidase-IV (DPP-IV) and angiotensin-I-converting enzyme (ACE-I) inhibitory activities. Pepsin (pH > 2) was predicted to be the most promising enzyme for the production of bioactive peptides from GR protein, which theoretically released 82 DPP-IV inhibitory peptides and 47 ACE-I inhibitory peptides. Overall, this work highlighted the potentiality of giant grouper roe as raw material for the generation of pharmaceutical products. Furthermore, the application of proteomics and in silico techniques provided rapid identification of proteins and useful prediction of its potential bioactivities.
Carbonic Anhydrase Inhibitors as Novel Drugs against Mycobacterial β-Carbonic Anhydrases: An Update on In Vitro and In Vivo Studies
Authors: Aspatwar Ashok , Winum Jean-Yves , Carta Fabrizio , Supuran Claudiu T. , Hammaren Milka , Parikka Mataleena , Parkkila Seppo
Published: November 2018

Mycobacteria cause a variety of diseases, such as tuberculosis, leprosy, and opportunistic diseases in immunocompromised people. The treatment of these diseases is problematic, necessitating the development of novel treatment strategies. Recently, β-carbonic anhydrases (β-CAs) have emerged as potential drug targets in mycobacteria. The genomes of mycobacteria encode for three β-CAs that have been cloned and characterized from Mycobacterium tuberculosis (Mtb) and the crystal structures of two of the enzymes have been determined. Different classes of inhibitor molecules against Mtb β-CAs have subsequently been designed and have been shown to inhibit these mycobacterial enzymes in vitro. The inhibition of these centrally important mycobacterial enzymes leads to reduced growth of mycobacteria, lower virulence, and impaired biofilm formation. Thus, the inhibition of β-CAs could be a novel approach for developing drugs against the severe diseases caused by pathogenic mycobacteria. In the present article, we review the data related to in vitro and in vivo inhibition studies in the field.
Heparan Sulfate Mimetics in Cancer Therapy: The Challenge to Define Structural Determinants and the Relevance of Targets for Optimal Activity
Authors: Lanzi Cinzia , Cassinelli Giuliana
Published: November 2018

Beyond anticoagulation, the therapeutic potential of heparin derivatives and heparan sulfate (HS) mimetics (functionally defined HS mimetics) in oncology is related to their ability to bind and modulate the function of a vast array of HS-binding proteins with pivotal roles in cancer growth and progression. The definition of structural/functional determinants and the introduction of chemical modifications enabled heparin derivatives to be identified with greatly reduced or absent anticoagulant activity, but conserved/enhanced anticancer activity. These studies paved the way for the disclosure of structural requirements for the inhibitory effects of HS mimetics on heparanase, selectins, and growth factor receptor signaling, as well as for the limitation of side effects. Actually, HS mimetics affect the tumor biological behavior via a multi-target mechanism of action based on their effects on tumor cells and various components of the tumor microenvironment. Emerging evidence indicates that immunomodulation can participate in the antitumor activity of these agents. Significant ability to enhance the antitumor effects of combination treatments with standard therapies was shown in several tumor models. While the first HS mimetics are undergoing early clinical evaluation, an improved understanding of the molecular contexts favoring the antitumor action in certain malignancies or subgroups is needed to fully exploit their potential.
Mitigation Effects of a Novel Herbal Medicine, Hepad, on Neuroinflammation, Neuroapoptosis, and Neuro-Oxidation
Authors: Song Da Hye , Kim Gyeong-Ji , Lee Kwon Jai , Shin Jae Soo , Kim Dong-Hee , Park Byung-Jun , An Jeung Hee
Published: November 2018

Parkinson’s disease (PD), a common adult-onset neurodegenerative disorder with complex pathological mechanisms, is characterized by the degeneration of dopaminergic nigrostriatal neurons. The present study demonstrated that the herbal medicines Hepad 1 and 2 protected against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced dopaminergic neurotoxicity in C57BL/6 mice and SH-SY5Y cells. Hepad 1 and 2 remarkably alleviated the enhanced expression of pro-inflammatory cytokines (tumor necrosis factor-α, interleukin-6, inducible nitric oxide synthase, cyclooxygenase-2, macrophage-1, and phosphorylated iκB-α) and apoptotic signals (Bcl-2-associated X protein, caspase-3, and poly [ADP-ribose] polymerase-1). Additionally, Hepad reduced MPTP-induced oxidative damage by increasing the expression of anti-oxidant defense enzymes (superoxide dismutase and glutathione S-transferase) and downregulating the levels of nicotinamide adenine dinucleotide phosphate oxidase 4. This study also showed that the neuroprotective effects of Hepad include anti-inflammatory, anti-apoptotic, and anti-oxidative properties, in addition to activation of the protein kinase B, extracellular-signal-regulated kinase, and c-Jun N-terminal kinase signaling pathways. Furthermore, oral administration of Hepad 1 and 2 attenuated the death of tyrosine hydroxylase-positive substantia nigra neurons that was induced by 20 mg/kg MPTP. Therefore, our results suggest that Hepad 1 and 2 are useful for treating PD and other disorders associated with neuro-inflammatory, neuro-apoptotic, and neuro-oxidative damage.
Highly Cancer Selective Antiproliferative Activity of Natural Prenylated Flavonoids
Authors: Bartmańska Agnieszka , Tronina Tomasz , Popłoński Jarosław , Milczarek Magdalena , Filip-Psurska Beata , Wietrzyk Joanna
Published: November 2018

Xanthohumol (XN) and four minor hops prenylflavonoids: α,β-dihydroxanthohumol (2HXN), isoxanthohumol (IXN), 8-prenylnaringenin (8PN), and 6-prenylnaringenin (6PN), were tested for antiproliferative activity towards human cancer and normal cell lines. Nonprenylated naringenin (NG) was used as a model compound. Xanthohumol, α,β-dihydroxanthohumol and 6-prenylnaringenin were the most active compounds. Xanthohumol exhibited higher antiproliferative activity than cisplatin (CP) against five cancer cell lines: ovarian resistant to cisplatin A2780cis, breast MDA-MB-231 and T-47D, prostate PC-3, and colon HT-29. Isoxanthohumol was more potent than cisplatin against breast cancer cell lines MDA-MB-231 and T-47D whereas 6-prenylnaringenin was stronger than cisplatin against breast cancer cell line T-47D. It was found that tested chalcones possessed highly selective antiproliferative activity towards all tested breast cancer lines compared to the normal breast MCF 10A cell line (the calculated selectivity index ranged from 5 to 10). Low antiproliferative activity of naringenin indicates the importance of the prenyl group with respect to antiproliferative activity.
Macroscopic Observation of Soil Nitrification Kinetics Impacted by Copper Nanoparticles: Implications for Micronutrient Nanofertilizer
Authors: VandeVoort Allison Rick , Arai Yuji
Published: November 2018

The potential agricultural use of metal nanoparticles (NPs) for slow-release micronutrient fertilizers is beginning to be investigated by both industry and regulatory agencies. However, the impact of such NPs on soil biogeochemical cycles is not clearly understood. In this study, the impact of commercially-available copper NPs on soil nitrification kinetics was investigated via batch experiments. The X-ray absorption near edge structure spectroscopy analysis showed that the NPs readily oxidized to Cu(II) and were strongly retained in soils with minimum dissolution (<1% of total mass). The Cu2+ (aq) at 1 mg/L showed a beneficial effect on the nitrification similar to the control: an approximately 9% increase in the average rate of nitrification kinetics (Vmax). However Vmax was negatively impacted by ionic Cu at 10 to 100 mg/L and CuNP at 1 to 100 mg/L. The copper toxicity of soil nitrifiers seems to be critical in the soil nitrification processes. In the CuNP treatment, the suppressed nitrification kinetics was observed at 1 to 100 mg/kg and the effect was concentration dependent at ≥10 mg/L. The reaction products as the results of surface oxidation such as the release of ionic Cu seem to play an important role in suppressing the nitrification process. Considering the potential use of copper NPs as a slow-release micronutrient fertilizer, further studies are needed in heterogeneous soil systems.
Degradable and Photocatalytic Antibacterial Au-TiO2/Sodium Alginate Nanocomposite Films for Active Food Packaging
Authors: Tang Siying , Wang Zhe , Li Penghui , Li Wan , Li Chengyong , Wang Yi , Chu Paul K.
Published: November 2018

A degradable and antibacterial sodium alginate film containing functional Au-TiO2 nanocomposites for food packaging was successfully developed. The Au-TiO2 nanocomposites are synthesized hydrothermally and mixed with the alginate solution to form the film by a casting method. The Au-TiO2 nanocomposites enable the film with excellent visible light absorption and transfer ability with the light absorption rang covering UV–visible wavelength (300–800 nm) and induce the increase of the film water contact angle from 40° to 74°, which contributes to the film shape stability. Furthermore, compared to the TiO2 nanoparticle-incorporated film, the antibacterial ability of Au-TiO2/sodium alginate composite film is improved approximately by 60% and 50% against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli), respectively, in light conditions. The antibacterial property of the film arises from the increased production of reactive oxygen species (ROS) induced by the surface plasmonic resonance of Au nanoparticles. The degradable and antibacterial properties render the composite film of great application potential in food packaging industry.
Almond Allergy: An Overview on Prevalence, Thresholds, Regulations and Allergen Detection
Authors: Mandalari Giuseppina , Mackie Alan R.
Published: November 2018

Food allergy has been on the increase for many years. The prevalence of allergy to different foods varies widely depending on type of food, frequency of consumption and geographic location. Data from the literature suggests that the prevalence of tree nut allergy is of the order of 1% in the general population. Almond is one such tree nut that is frequently eaten in many parts of the world and represents a potential allergenic hazard. Given the need to label products that contain allergens, a number of different methods of direct and indirect detection have been developed. However, in the absence of population-based threshold data, and given that almond allergy is rare, the sensitivity of the required detection is unknown and thus aims as low as possible. Typically, this is less than 1 ppm, which matches the thresholds that have been shown for other allergens. This review highlights the lack of quantitative data on prevalence and thresholds for almonds, which is limiting progress in consumer protection.
The Association of Poor Oral Health Parameters with Malnutrition in Older Adults: A Review Considering the Potential Implications for Cognitive Impairment
Authors: Kossioni Anastassia E.
Published: November 2018

Poor dental status and chewing deficiencies have been associated with cognitive decline. Altered dietary habits and malnutrition have been suggested as linking mechanisms. The aim of the present review was thus to investigate if poor oral health, and in particular tooth loss and impaired masticatory function, may affect dietary selection and nutritional intake in older adults, and moreover, to assess if prosthodontic dental care may improve nutritional status. Extensive tooth loss may impair masticatory function. Several studies in older populations have shown that severe tooth loss and masticatory impairment are associated with limited consumption of various food types (especially fruits and vegetables), increased consumption of sugary and easy-to-chew foods, and lower dietary intake of fibre and vitamins. However, these findings are not consistently reported, due to methodological variation among studies, potential adverse causalities, and the multifactorial nature of food choices. On the other hand, a few interventional studies revealed that prosthetic rehabilitation of missing teeth, when accompanied by dietary counselling, may improve dietary habits and nutritional intake. Further research is required to improve current knowledge of these associations. Under the limitations of the current study, a functional dental arch of natural or artificial teeth is important for maintaining adequate chewing efficiency and ability, but this only partly contributes to food choices and nutritional status. The multifactorial nature of food choices necessitates the interprofessional collaboration of dental professionals, dietetics practitioners, and primary care providers to improve dietary habits and nutritional intake.
Celiac Male’s Gluten-Free Diet Profile: Comparison to that of the Control Population and Celiac Women
Authors: González Teba , Larretxi Idoia , Vitoria Juan Carlos , Castaño Luis , Simón Edurne , Churruca Itziar , Navarro Virginia , Lasa Arrate
Published: November 2018

The aim of the present work was to analyze the body composition and dietary profile of Spanish celiac men and to compare them to control men and celiac women from our previous studies. Forty-two celiac men (31.5 ± 11.9 years) were recruited and anthropometric measurements were taken. Analysis of energy consumption, macro- and micronutrient intake and food frequency consumption was carried out. Celiac men were more overweight and obese than celiac women, but less than the control population, reporting the same energy intake and macronutrient distribution. Most micronutrient deficiencies in celiac men were not directly related to a gluten free diet; these were also observed for the entire population. The least adherence to Dietary Reference Intakes in women was reported for iron, iodine, potassium and selenium, whereas magnesium intake was higher than in men. Among celiac participants (both genders), cereal, vegetable and legume consumption was poor and meat intake was contrastingly excessive. In conclusion, the dietary profile of celiac men is as unbalanced as that of control men but slightly more than that of celiac women. General nutritional education should be given to both general and celiac populations, and specific advices to celiac men, in order to decrease the risk of celiac disease-related pathologies.
A Review on Liquid Chromatography-Tandem Mass Spectrometry Methods for Rapid Quantification of Oncology Drugs
Authors: Wong Andrea Li-Ann , Xiang Xiaoqiang , Ong Pei Shi , Mitchell Ee Qin Ying , Syn Nicholas , Wee Ian , Kumar Alan Prem , Yong Wei Peng , Sethi Gautam , Goh Boon Cher , Ho Paul Chi-Lui , Wang Lingzhi
Published: November 2018

In the last decade, the tremendous improvement in the sensitivity and also affordability of liquid chromatography-tandem mass spectrometry (LC-MS/MS) has revolutionized its application in pharmaceutical analysis, resulting in widespread employment of LC-MS/MS in determining pharmaceutical compounds, including anticancer drugs in pharmaceutical research and also industries. Currently, LC-MS/MS has been widely used to quantify small molecule oncology drugs in various biological matrices to support preclinical and clinical pharmacokinetic studies in R&D of oncology drugs. This mini-review article will describe the state-of-the-art LC-MS/MS and its application in rapid quantification of small molecule anticancer drugs. In addition, efforts have also been made in this review to address several key aspects in the development of rapid LC-MS/MS methods, including sample preparation, chromatographic separation, and matrix effect evaluation.
The Question of Nationalism and Belonging
Authors: Buyze David
Published: November 2018

This paper situates an analysis on the commonalities and ordinariness of Jewish and Muslim experiences vis-à-vis a critique on nationalism and belonging in the literature of Edeet Ravel and Mohsin Hamid, in addition to other writers. These literary writers are highlighted by an exploration of Eran Riklis’ film A Borrowed Identity amidst the critical perspectives of Ari Shavit, Leila Ahmed, Edward W. Said, and Justin Trudeau. The focus on Israel/Palestine is complemented by addressing sustained issues of nationalism and belonging in America that reverberate on global degrees of awareness as to how religious degrees of belonging can be reconsidered in light of understanding instantiations of cultural mise-en-scène from nuanced degrees of awareness. In turn, a multifaceted unsettling of identity, religion, and culture is posited that vividly collapses distinctions between East/West in revealing highly different ways of contemplating perceptions of Jews and Muslims in the world today.
On the Optimal Design of Doppler Scatterometers
Authors: Rodriguez Ernesto
Published: November 2018

Pencil-beam Doppler scatterometers are a promising remote sensing tool for measuring ocean vector winds and currents from space. While several point designs exist in the literature, these designs have been constrained by the hardware they inherited, and the design is sub-optimal. Here, guidelines to optimize the design of these instruments starting from the basic sensitivity equations are presented. Unlike conventional scatterometers or pencil-beam imagers, appropriate sampling of the Doppler spectrum and optimizing the radial velocity error lead naturally to a design that incorporates a pulse-to-pulse separation and pulse length that vary with scan angle. Including this variation can improve radial velocity performance significantly and the optimal selection of system timing and bandwidth is derived. Following this, optimization of the performance based on frequency, incidence angle, antenna length, and spatial sampling strategy are considered. It is shown that antenna length influences the performance most strongly, while the errors depend only on the square root of the transmit power. Finally, a set of example designs and associated performance are presented.
Canopy Effects on Snow Accumulation: Observations from Lidar, Canonical-View Photos, and Continuous Ground Measurements from Sensor Networks
Authors: Zheng Zeshi , Ma Qin , Qian Kun , Bales Roger C.
Published: November 2018

A variety of canopy metrics were extracted from the snow-off airborne light detection and ranging (lidar) measurements over three study areas in the central and southern Sierra Nevada. Two of the sites, Providence and Wolverton, had wireless snow-depth sensors since 2008, with the third site, Pinecrest having sensors since 2014. At Wolverton and Pinecrest, images were captured and the sky-view factors were derived from hemispherical-view photos. We found the variation of snow accumulation across the landscape to be significantly related to canopy-cover conditions. Using a regularized regression model Elastic Net to model the normalized snow accumulation with canopy metrics as independent variables, we found that about 50 % of snow accumulation variability at each site can be explained by the canopy metrics from lidar.
Tropical Cyclone Rainfall Estimates from FY-3B MWRI Brightness Temperatures Using the WS Algorithm
Authors: Zhang Ruanyu , Wang Zhenzhan , Hilburn Kyle A.
Published: November 2018

A rainfall retrieval algorithm for tropical cyclones (TCs) using 18.7 and 36.5 GHz of vertically and horizontally polarized brightness temperatures (Tbs) from the Microwave Radiation Imager (MWRI) is presented. The beamfilling effect is corrected based on ratios of the retrieved liquid water absorption and theoretical Mie absorption coefficients at 18.7 and 36.5 GHz. To assess the performance of this algorithm, MWRI measurements are matched with the National Snow and Ice Data Center (NSIDC) precipitation for six TCs. The comparison between MWRI and NSIDC rain rates is relatively encouraging, with a mean bias of −0.14 mm/h and an overall root-mean-square error (RMSE) of 1.99 mm/h. A comparison of pixel-to-pixel retrievals shows that MWRI retrievals are constrained to reasonable levels for most rain categories, with a minimum error of −1.1% in the 10–15 mm/h category; however, with maximum errors around −22% at the lowest (0–0.5 mm/h) and highest rain rates (25–30 mm/h). Additionally, Advanced Microwave Scanning Radiometer for Earth Observing System (AMSR-E) Tbs are applied to retrieve rain rates to assess the sensitivity of this algorithm, with a mean bias and RMSE of 0.90 mm/h and 3.11 mm/h, respectively. For the case study of TC Maon (2011), MWRI retrievals underestimate rain rates over 6 mm/h and overestimate rain rates below 6 mm/h compared with Precipitation Radar (PR) observations on storm scales. The Tropical Rainfall Measuring Mission (TRMM) Microwave Imager (TMI) rainfall data provided by the Remote Sensing Systems (RSS) are applied to assess the representation of mesoscale structures in intense TCs, and they show good consistency with MWRI retrievals.
Underestimates of Grassland Gross Primary Production in MODIS Standard Products
Authors: Zhu Xiaoyan , Pei Yanyan , Zheng Zhaopei , Dong Jinwei , Zhang Yao , Wang Junbang , Chen Lajiao , Doughty Russell B. , Zhang Geli , Xiao Xiangming
Published: November 2018

As the biggest carbon flux of terrestrial ecosystems from photosynthesis, gross primary productivity (GPP) is an important indicator in understanding the carbon cycle and biogeochemical process of terrestrial ecosystems. Despite advances in remote sensing-based GPP modeling, spatial and temporal variations of GPP are still uncertain especially under extreme climate conditions such as droughts. As the only official products of global spatially explicit GPP, MOD17A2H (GPPMOD) has been widely used to assess the variations of carbon uptake of terrestrial ecosystems. However, systematic assessment of its performance has rarely been conducted especially for the grassland ecosystems where inter-annual variability is high. Based on a collection of GPP datasets (GPPEC) from a global network of eddy covariance towers (FluxNet), we compared GPPMOD and GPPEC at all FluxNet grassland sites with more than five years of observations. We evaluated the performance and robustness of GPPMOD in different grassland biomes (tropical, temperate, and alpine) by using a bootstrapping method for calculating 95% confident intervals (CI) for the linear regression slope, coefficients of determination (R2), and root mean square errors (RMSE). We found that GPPMOD generally underestimated GPP by about 34% across all biomes despite a significant relationship (R2 = 0.66 (CI, 0.63–0.69), RMSE = 2.46 (2.33–2.58) g Cm−2 day−1) for the three grassland biomes. GPPMOD had varied performances with R2 values of 0.72 (0.68–0.75) (temperate), 0.64 (0.59–0.68) (alpine), and 0.40 (0.27–0.52) (tropical). Thus, GPPMOD performed better in low GPP situations (e.g., temperate grassland type), which further indicated that GPPMOD underestimated GPP. The underestimation of GPP could be partly attributed to the biased maximum light use efficiency (εmax) values of different grassland biomes. The uncertainty of the fraction of absorbed photosynthetically active radiation (FPAR) and the water scalar based on the vapor pressure deficit (VPD) could have other reasons for the underestimation. Therefore, more accurate estimates of GPP for different grassland biomes should consider improvements in εmax, FPAR, and the VPD scalar. Our results suggest that the community should be cautious when using MODIS GPP products to examine spatial and temporal variations of carbon fluxes.
The Making of Democratic Actors: Counting the Costs of Public Cuts in England on Young People’s Steps towards Citizenship
Authors: Sharpe Darren
Published: November 2018

This paper provides a synthesis of qualitative studies, examining youth empowerment projects and initiatives that have encouraged young people to have a voice in local, regional, and national political debates. Specifically, the article examines the role of English youth services in building the spirit of citizenship in young people against the challenging question of the changing behavioural pattern and profiles of young English electorates. To do this, the paper draws on four case studies to help rethink the critical moments for disadvantaged and vulnerable young people in their journeys towards citizenship, and how English youth services understand and respond to the experiences of young people. The article presents the strengths and limitations of the youth sector to enrich and furnish the spirit of citizenship in today’s youth, and argues for a more innovative role in the part played by the state in an era of austerity.
Conservation Agriculture Using Coulters: Effects of Crop Residue on Working Performance
Authors: Wang Qi , Zhu Longtu , Li Mingwei , Huang Dongyan , Jia Honglei
Published: November 2018

Conservation agriculture is an important measure promoting sustainable agriculture in northeast China. Coulters in the conservation agriculture system are used to cut the excessive residue in strips, loosen soils, and create good seedbeds. Information on the performance of coulters worked in a field with or without corn residue coverage is lacking in the literature. In this study, five coulters were tested in two field conditions at three working velocities to compare their performance. The five coulters were four types of fluted coulters (8 W, 13 W, 18 W, and 25 W) and one notched-flat coulter (NF); the two field conditions were whole residue plots (WR) and no residue plots(NR), and the three working velocities were 8 km/h (V1), 10 km/h (V2), and 12 km/h (V3). All of the tests were tested at a tillage depth of 80 mm. The results showed that the maximum furrow width (Wf), furrow disturbance area (A), and residue coverage change (C) were significantly affected by the working velocity and coulter type, while the cutting force (F) and skid rate (S) were significantly affected by the residue coverage, working velocity, and coulter type. The NF was found to have the smallest furrow profile, residue coverage change, and cutting force, as well as the largest skid rate. Among the fluted coulters, as the wavenumber rose, the cutting force, furrow width, and furrow disturbance area all gradually decreased, while the skid rate and residue coverage change were gradually enhanced. The straw residual intensified the cutting force and reduced the skid rate, which changed by 11.6% and 20.9%, respectively. As the working velocity rose from 8 km/h to 12 km/h, the furrow width, furrow disturbance area, residue coverage change, cutting force, and skid rate increased by 26.5%, 16.5%, 44.6%, 8.2%, and 22.7%, respectively. The results reveal that the flat coulter and large-wavenumber fluted coulters (18 W and 25 W) have less cutting force and are more beneficial for cutting straw residue in residue coverage fields, while the small-wavenumber fluted coulters (8 W and 13 W) are suitable for loosening soil and constructing seedbeds. The cutting force has significant effects on the performance of cutting straw residue, loosening soils, and creating seedbeds.
Assessment of Heavy Metals and Color as Indicators of Contamination in Street Dust of a City in SE Spain: Influence of Traffic Intensity and Sampling Location
Authors: Marín Sanleandro Pura , Sánchez Navarro Antonio , Díaz-Pereira Elvira , Bautista Zuñiga Francisco , Romero Muñoz Miriam , Delgado Iniesta María José
Published: November 2018

In the present work, a sampling grid of the urban core of the city of Murcia (South East Spain) was designed in order to analyze street dust, focusing on the contents of the heavy metals Cd, Cr, Cu, Ni, Pb, and Zn and their relationships with the color of the sample, the traffic pattern, and the location where they were sampled (sidewalks, ledges, and roads). The characterization of the samples was carried out by X-ray diffraction and scanning electron microscopy, whereas the heavy metals were extracted by acid digestion and determined by inductively coupled plasma mass spectrometry. The concentration (mg/kg) in urban dust of the city of Murcia was highest for Zn (653), followed by Cu (201) > Pb (177) > Cr (117) > Ni (51) >> Cd (0.5). The color expounded statistically significant differences with regard to the heavy metals, including the pollutant load. The same pattern was found when the classification variable was the traffic intensity, except in the case of Ni. The areas with a higher risk of contamination by heavy metals in the urban dust are the ledges of narrow city center streets with moderate traffic, where Zn and Pb seem to accumulate most greatly.
Leading from the Niche: Insights from a Strategic Dialogue of Renewable Energy Cooperatives in The Netherlands
Authors: Proka Antonia , Loorbach Derk , Hisschemöller Matthijs
Published: November 2018

Renewable energy cooperatives envision and manifest an alternative way of organising within the energy system (and beyond). Yet, despite their growth, it is uncertain whether such initiatives are able to increase and deepen their impact, leading the transition to an environmentally sustainable and socially just energy system. This paper presents insights from a strategic dialogue co-organised with the Dutch national interest group of renewable energy cooperatives “ODE Decentraal”. We used transition management as action research methodology to organise the dialogue to understand and support the transformative potential of the cooperative energy movement. The dialogue helped to clarify the challenges and possibilities for scaling energy cooperatives beyond the niche, supporting at the same time the participants to reflect, strategize and develop a shared transition agenda. This contribution presents and analyses our intervention and its impact, also specifically evaluating the potential of transition management to facilitate social learning processes, reflexivity and the development of strategic actions. Our intervention confirmed the hypothesis that actors in the niche often focus too much on the competition with the regime for individual goals, thereby failing to collectively strategize and engage with incumbent regimes in a systematic way.
Toward Sustainable Biofuels in the European Union? Lessons from a Decade of Hybrid Biofuel Governance
Authors: Stattman Sarah L. , Gupta Aarti , Partzsch Lena , Oosterveer Peter
Published: November 2018

The European Union (EU) stands at a crossroads regarding its biofuel policies. For more than a decade, the EU sought to create a market for and govern sustainable biofuels for the transport sector, even as debates over sustainability escalated. It did so by devising novel hybrid (public and private) governance arrangements. We took stock of the nature and outcomes of this experiment in hybrid biofuel governance. We relied on qualitative methods of analysis, whereby we reviewed and synthesized the evolution of EU biofuel governance arrangements over time, through detailed document analysis of secondary and primary literature, including EU and related policy documents and private certification scheme websites. Our analysis reveals that, instead of yielding an increasingly stringent sustainability framework, the hybrid EU governance arrangements resulted in a proliferation of relatively lax, industry-driven, sustainability standards, even as the notion of “sustainable biofuels” remained contested in public and political debate. These findings contribute to an ongoing debate about the merits of hybrid (public–private) governance arrangements, and whether a hybrid approach helps strengthen or weaken sustainability objectives. We conclude that a more stringent EU meta-standard on sustainability needs to be developed, to underpin future governance arrangements.
Qualitative Analysis of Traditional Italian Dishes: FTIR Approach
Authors: Durazzo Alessandra , Kiefer Johannes , Lucarini Massimo , Camilli Emanuela , Marconi Stefania , Gabrielli Paolo , Aguzzi Altero , Gambelli Loretta , Lisciani Silvia , Marletta Luisa
Published: November 2018

Italian cuisine and its traditional recipes experience an ever-increasing popularity around the world. The “Integrated Approach” is the key to modern food research and the innovative challenge for analyzing and modeling agro-food systems in their totality. The present study aims at applying and evaluating Fourier Transformed Infrared (FTIR) spectroscopy for the analysis of complex food matrices and food preparations. Nine traditional Italian recipes, including First courses, One-dish meals, Side courses, and Desserts, were selected and experimentally prepared. Prior to their analysis via FTIR spectroscopy, the samples were homogenized and lyophilized. The IR spectroscopic characterization and the assignment of the main bands was carried out. Numerous peaks, which correspond to functional groups and modes of vibration of the individual components, were highlighted. The spectra are affected by both the preparation procedures, the cooking methods, and the cooking time. The qualitative analysis of the major functional groups can serve as a basis for a discrimination of the products and the investigation of fraud. For this purpose, the FTIR spectra were evaluated using Principal Component Analysis (PCA). Our results show how the utilization of vibrational spectroscopy combined with a well-established chemometric data analysis method represents a potentially powerful tool in research linked to the food sector and beyond. This study is a first step towards the development of new indicators of food quality.
Nonuniformity Correction of Single Infrared Images Based on Deep Filter Neural Network
Authors: Jian Xianzhong , Lv Chen , Wang Ruzhi
Published: November 2018

The fixed-pattern noise (FPN) caused by nonuniform optoelectronic response limits the sensitivity of an infrared imaging system and severely reduces the image quality. Therefore, nonuniform correction of infrared images is very important. In this paper, we propose a deep filter neural network to solve the problems of network underfitting and complex training with convolutional neural network (CNN) applications in nonuniform correction. Our work is mainly based on the idea of deep learning, where the nonuniform image noise features are fully learned from a large number of simulated training images. The network is designed by introducing the filter and the subtraction structure. The background interference of the image is removed by the filter, so the learning model is gathered in the nonuniform noise. The subtraction structure is used to further reduce the input-to-output mapping range, which effectively simplifies the training process. The results from the test on infrared images shows that our algorithm is superior to the state-of-the-art algorithm in visual effects and quantitative measurements, providing a new method for deep learning in nonuniformity correction of single images.
Comparative Assessment of Tungsten Toxicity in the Absence or Presence of Other Metals
Authors: Wasel Ola , Freeman Jennifer L.
Published: November 2018

Tungsten is a refractory metal that is used in a wide range of applications. It was initially perceived that tungsten was immobile in the environment, supporting tungsten as an alternative for lead and uranium in munition and military applications. Recent studies report movement and detection of tungsten in soil and potable water sources, increasing the risk of human exposure. In addition, experimental research studies observed adverse health effects associated with exposure to tungsten alloys, raising concerns on tungsten toxicity with questions surrounding the safety of exposure to tungsten alone or in mixtures with other metals. Tungsten is commonly used as an alloy with nickel and cobalt in many applications to adjust hardness and thermal and electrical conductivity. This review addresses the current state of knowledge in regard to the mechanisms of toxicity of tungsten in the absence or presence of other metals with a specific focus on mixtures containing nickel and cobalt, the most common components of tungsten alloy.
Regeneration and Reuse of Immunoaffinity Column for Highly Efficient Clean-Up and Economic Detection of Ochratoxin A in Malt and Ginger
Authors: Liu Xi , Liu Xiaofei , Huang Pinxuan , Wei Fang , Ying Guangyao , Lu Jinghua , Zhou Lidong , Kong Weijun
Published: November 2018

Immunoaffinity columns (IACs) are most popularly used for mycotoxin clean-up in complex matrices prior to chromatographic analysis. But, their high cost has limited their wide application and the regeneration of IACs for multiple instances of reuse is important. This study aimed to investigate the feasibility of regeneration and reuse of IACs for purification of ochratoxin A (OTA) in spiked raw malt and dried ginger samples followed by high performance liquid chromatography-fluorescence detection. After each use, the IACs were filled with phosphate buffer saline (PBS) as the preservation solution and stored at 8 °C overnight for regeneration and reuse until the recovery rate was <70%. The results showed that matrix type, preparation procedure, and pH value of sample extraction exhibited major effects on the reuse of IACs for OTA clean-up. While, after modifying the sample preparation procedure using water as the diluent and the solution at a pH of 7 to 8, the IACs could be used eight and three times for the spiked raw malt and dried ginger samples with OTA after regeneration. Regarding the traditional procedure recommended in Chinese Pharmacopoeia (2015 edition), the IACs could be used for three and two times for the spiked raw malt and dried ginger samples with OTA, respectively. Therefore, the corresponding experimental cost could be reduced to one-eighth and one-third of the original cost. This is the first study on the regeneration and reuse of IACs for OTA clean-up in complex Chinese herbal medicines, providing a green and economical tool for a large number of samples analysis with low cost.
Optimal Scale of Urbanization with Scarce Water Resources: A Case Study in an Arid and Semi-Arid Area of China
Authors: Liu Siyuan , Wang Ni , Xie Jiancang , Jiang Rengui , Zhao Menglong
Published: November 2018

A complex interwoven relationship exists between water resources and urbanization, which is of much interest in international water science research. To study the urban development in large cities facing water deficiency problems, it is important to identify rational thresholds of urbanization to achieve optimal utilization of limited water resources, and to promote sustainable economic and population growth and social development. A multi-objective optimization model is proposed to search for the optimal scale of urbanization of large cities with limited water resources. To solve a large-dimensional multi-objective optimization problem, the non-dominated sorting genetic algorithm (NSGA-II) is improved to the OENSGA-II based on the orthogonal generation method and the E-optimality method and applied to a typical arid city, Xi’an of China, which underwent rapid urbanization in recent years. For Xi’an, a statistically significant positive correlation is found between urbanization rate (Ur) and gross domestic product (GDP), domestic water, tertiary industry water, and ecological water. However, Ur is negatively correlated with the primary industry water. If the current urbanization trend continues, the water resources available are far from sufficient to support the future city of Xi’an. In this work, it was found that, by implementing restrictive water resources management and water saving measures, the economic threshold of Xi’an could be raised to 1890.3 and 2403.3 billion yuan, while the population threshold could be raised to 11.0 and 13.9 million, and Ur to 79.9% and 85.9% in 2020 and 2030, respectively. The corresponding maximum urban area to be constructed based on the projected population will be 964.81 and 1197.6 km2 in those years. It will be prudent for Xi’an to practice strict water resource management, and to allocate its water resources among various water sectors effectively and equitably to avoid major water shortage problems in the future.
Quelques aspects des conditions de circulation des eaux souterraines dans les "karsts" du gypse des Alpes internes
Authors: Couturier Bernard , Fourneaux Jean-Claude , Sommeria Laure
Published: November 2018

Les affleurements de gypses sont nombreux dans les Alpes internes où ils jalonnent souvent les contacts entre unités structurales différentes et plus particulièrement les chevauchements. Une morphologie caractéristique avec dolines et zones d'effondrement souligne souvent leur présence même lorsqu'ils sont masqués par des dépôts morainiques ou des éboulis. Dans ces zones on peut aussi observer des pertes ce qui traduit l'existence de circulations souterraines. Dans certains cas il n'existe pas de résurgences à caractères karstiques que l'on puisse associer à ces pertes.

A partir de deux exemples de ce type où les gypses représentent la semelle d'un chevauchement, il est possible de voir que le contexte tectonique joue un grand rôle dans les conditions de circulation des eaux souterraines.

À La Norma, en Maurienne, les eaux infiltrées dans une vaste doline ressortent en différents points après un passage au sein d'une zone à perméabilité de pores. Aucune des sources n'a un débit important et variable qui soit caractéristique des exutoires karstiques. Sur le site du Clou, en haute Tarentaise, les eaux infiltrées dans plusieurs dolines se mélangent avec des eaux ayant une autre origine et subissent ensuite une grande dispersion. Deux traçages réalisés à différentes époques ont

montré que ces eaux ressortaient à plusieurs sources qui jalonnent un grand contact anormal.

Si la partie alimentation des réseaux est tout à fait analogue à celle des réseaux karstiques classiques que l'on rencontre en milieu calcaire, il n'en est pas de même au niveau des exutoires. Dans les deux exemples étudiés la structure des gypses induit une grande dispersion et la plupart des sources ne peuvent être assimilées à des résurgences. Ces karsts semblent se vidanger dans des nappes de fissures. Cela explique l'absence de véritables résurgences. Ces nappes de Assures jouent, par ailleurs, un rôle régulateur.

L'écoulement des eaux souterraines se fait comme s'il y avait passage continu d'un système karstique à un milieu fissuré sans chenaux et même par endroits à un milieu poreux. Cela se traduit sur les débits et sur la qualité des eaux par la disparition des traces de contamination.

Ce type de circulations semble lié aux zones où la tectonique ne permet pas le développement en profondeur des réseaux de chenaux. Il y a d'une part une sorte de compression qui empêche cette formation, mais aussi un écaillage qui entraîne une interpénétration des gypses et des cargneules avec les Schistes lustrés qui représentent dans les exemples étudiés la série chevauchante comme nous avons pu l'observer dans les galeries de reconnaissance sur le site du Clou.
Current Landscape of Immunotherapy in the Treatment of Solid Tumours, with Future Opportunities and Challenges
Authors: Nixon N. A. , Blais N. , Ernst S. , Kollmannsberger C. , Bebb G. , Butler M. , Smylie M. , Verma S.
Published: November 2018

Immunotherapy has emerged as a new standard of care, showing survival benefit for solid tumours in multiple disease sites and indications. The survival improvements seen in diseases that were highly resistant to traditional therapies, with a poor prognosis, are unprecedented. Although the benefits observed in clinical trials are undeniable, not all patients derive those benefits, leading to emerging combination strategies and an ongoing quest for biomarker selection. Here, we summarize the current evidence for immunotherapy in the treatment of solid tumours, and we discuss emerging strategies at the forefront of research. We discuss future challenges that will be encountered as experience and knowledge continue to expand in this rapidly emerging field.
Is There a Role for Adjuvant Therapy After Surgery in “High Risk for Recurrence” Kidney Cancer? an Update on Current Concepts
Authors: Sharma T. , Tajzler C. , Kapoor A.
Published: November 2018

Background: Although surgical resection remains the standard of care for localized kidney cancers, a significant proportion of patients experience systemic recurrence after surgery and hence might benefit from effective adjuvant therapy. So far, several treatment options have been evaluated in adjuvant clinical trials, but only a few have provided promising results. Nevertheless, with the recent development of targeted therapy and immunomodulatory therapy, a series of clinical trials are in progress to evaluate the potential of those novel agents in the adjuvant setting. In this paper, we provide a narrative review of the progress in this field, and we summarize the results from recent adjuvant trials that have been completed. Methods: A literature search was conducted. The primary search strategy at the medline, Cochrane reviews, and http://ClinicalTrials.gov/databases included the keywords “adjuvant therapy,” “renal cell carcinoma,” and “targeted therapy or/and immunotherapy.” Conclusions: Data from the S-TRAC study indicated that, in the “highest risk for recurrence” patient population, disease-free survival was increased with the use of adjuvant sunitinib compared with placebo. The assure trial showed no benefit for adjuvant sunitinib or sorafenib in the “intermediate- to high-risk” patient population. The ARISER (adjuvant girentuximab) and PROTECT (adjuvant pazopanib) trials indicated no survival benefit, but subgroup analyses in both trials recommended further investigation. The inconsistency in some of the current results can be attributed to a variety of factors pertaining to the lack of standardization across the trials. Nevertheless, patients in the “high risk of recurrence” category after surgery for their disease would benefit from a discussion about the potential benefits of adjuvant treatment and enrolment in ongoing adjuvant trials.