bluuebunny/crossref_metadata_2025 huggingface dataset:
Research on High-Dose-Rate Transient Ionizing Radiation Effect in Nano-Scale FDSOI Flip-Flops
Authors: Li Tongde , Yuan Jingshuang , Bai Yang , Yu Chunqing , Gou Chunliang , Shu Lei , Wang Liang , Zhao Yuanfu
Published: July 2023

This paper presents an experimental study on the high-dose-rate transient ionizing radiation response and influencing factors of a Nano-Scale Fully Depleted Silicon-On-Insulator (FDSOI) D flip-flops (DFFs) circuit. Results indicate that data errors occur in DFFs at the lowest dose rate of 4.70 × 1011 rad(Si)/s in experiments, and the number of data errors shows a nonlinear increasing trend with the increase in dose rate and supply voltage. Three-dimensional technology computer-aided design (TCAD) simulations were conducted to analyze the transient photocurrent and charge collection mechanism at advanced process. The simulation results indicated that the charge collection efficiency is heightened with an increase in supply voltage, resulting in the higher photocurrent. This plays a major role in the process of charge collection for Ultra-Thin Body and Buried oxide (UTBB) FDSOI technology. The investigation into the high-dose-rate transient ionizing radiation effect (HDR-TIRE) in Nano-Scale FDSOI DFFs will aid in the assessment and application of advanced integrated circuits in aerospace.
Experimental Performance of a Novel Dual−Stage Counter−Rotating Small Wind Turbine and Forming a Validatable CFD Computational Model
Authors: Le Dang Huy , Nguyen The Bao , Ngo Van Minh
Published: July 2023

This paper publishes the experimental performance results of a novel small dual−stage wind turbine at different speeds under wind tunnel testing. The results confirm its superiority at low wind speeds <7 m/s, which is consistent with natural wind conditions in most countries around the world. At lower wind speeds, the efficiency of the small, dual−stage wind turbine is higher than that of a conventional single−stage turbine. At wind speeds of <6 m/s, the novel dual−stage wind turbine always produces approximately 2.5 times more power than a single−stage wind turbine; the power coefficient is greater than 0.4. Thanks to this feature, the novel small, dual−stage wind turbine is effective in practical applications. For windy areas in Vietnam, the power generated over the same amount of time by the novel dual−stage wind turbine can be more than double that of a single−stage turbine. Next, a standard CFD model is established and validated with the above experimental measurements. This method ensures the objectivity and authenticity of the computational model. This standard CFD model is applicable to a horizontal double−stage wind turbine with any configuration and blade profile. The URANS simulation method, which uses sliding meshes and the k−omega SST turbulence model, was implemented with rotational domains of the minimum thicknesses of rotational domains (in this paper, 0.09Rfront and 0.06Rrear, accordingly). This method predicts higher mechanical work for the turbine than the actual value by an interval of <10% in the same speed range as in the experiment. This is an acceptable deviation.
Analysis of the Contribution of China’s Car-Sharing Service to Carbon Emission Reduction
Authors: Cao Xinyue , Zhou Haizhu , Li Han , Kong Xiangfei
Published: July 2023

In light of carbon peak and carbon neutrality goals, China has attached great importance to energy savings and carbon reduction. Carbon reduction in the transport sector is critical to achieving the two-carbon target, as it accounts for 9.41% of total carbon emissions. As the sharing economy grows, car sharing is considered to present excellent carbon reduction potential in the transportation sector. However, the current research is focused on car sharing usage, with a lack of research on the carbon reduction capability of car sharing in China. Hence, this study aims to investigate the carbon reduction capacity of car sharing, including usage rates of car-share services and changes in travel behavior, through an online questionnaire combined with carbon emission data from the transportation sector. The study aims to analyze the contribution of car-share services to carbon reduction in the transportation sector under the current model. The well-to-wheel (WTW) approach is employed, including the energy consumption of vehicles and carbon emissions in the production process. The research results indicate that the introduction of car-sharing services increases driving energy consumption; however, this increase is offset by the decrease in carbon emissions as a result of the production process. Therefore, the overall effect is a reduction in carbon emissions of 1.058971 million tons in 2021, accounting for 1.95 percent of total transport carbon emissions. In addition, the impact on different modes on carbon emission reduction is also explored in this study. The results demonstrate that the private car disposal rate shows the most significant influence on traffic carbon emissions; a 10% reduction in the number of private cars can lead to a 2.48% carbon reduction. The relevant conclusions of this study can provide support for the future development of car sharing in China and the reduction of carbon emissions in the transportation sector.
Complete Chloroplast Genome Sequences of Two Ehretia Trees (Ehretia cymosa and Ehretia obtusifolia): Genome Structures and Phylogenetic Analysis
Authors: Alawfi Mohammad S. , Alzahrani Dhafer A. , Albokhari Enas J.
Published: July 2023

Ehretiaceae is a family in the order Boraginales. It contains more than 150 species. The Ehretiaceae classification has remained elusive and changed over time from subfamily to family, or vice versa. In this paper, we sequenced, characterized, and analyzed the complete chloroplast (cp) genomes of Ehretia cymosa and Ehretia obtusifolia, and their cp genomes were compared to those of related species. The length of the chloroplast genomes of E. cymosa was 156,328 bp, whereas that of E. obtusifolia was 155,961 bp. Each genome contained 114 genes, including 80 protein-coding genes, 4 rRNA genes, and 30 tRNA genes. Repeat analysis revealed that complement, forward, palindromic, and reverse repeats were present in the chloroplast genomes of both species. Simple sequence repeat analysis showed that the chloroplast genomes of E. cymosa and E. obtusifolia comprise 141 and 139 microsatellites, respectively. Phylogenetic analysis based on Bayesian and maximum likelihood analyses divided the order Boraginales into two well-supported clades. The first clade includes a single family (Boraginaceae), and the second clade includes three families (Ehretiaceae, Cordiaceae, and Heliotropiaceae). This study provides valuable genomic resources and insights into the evolutionary relationships within Boraginales.
ranchSATdb: A Genome-Wide Simple Sequence Repeat (SSR) Markers Database of Livestock Species for Mutant Germplasm Characterization and Improving Farm Animal Health
Authors: Duhan Naveen , Kaur Simardeep , Kaundal Rakesh
Published: July 2023

Microsatellites, also known as simple sequence repeats (SSRs), are polymorphic loci that play an important role in genome research, animal breeding, and disease control. Ranch animals are important components of agricultural landscape. The ranch animal SSR database, ranchSATdb, is a web resource which contains 15,520,263 putative SSR markers. This database provides a comprehensive tool for performing end-to-end marker selection, from SSRs prediction to generating marker primers and their cross-species feasibility, visualization of the resulting markers, and finding similarities between the genomic repeat sequences all in one place without the need to switch between other resources. The user-friendly online interface allows users to browse SSRs by genomic coordinates, repeat motif sequence, chromosome, motif type, motif frequency, and functional annotation. Users may enter their preferred flanking area around the repeat to retrieve the nucleotide sequence, they can investigate SSRs present in the genic or the genes between SSRs, they can generate custom primers, and they can also execute in silico validation of primers using electronic PCR. For customized sequences, an SSR prediction pipeline called miSATminer is also built. New species will be added to this website’s database on a regular basis throughout time. To improve animal health via genomic selection, we hope that ranchSATdb will be a useful tool for mapping quantitative trait loci (QTLs) and marker-assisted selection. The web-resource is freely accessible at https://bioinfo.usu.edu/ranchSATdb/.
Melkersson–Rosenthal Syndrome and Migraine: A New Phenotype Associated with SCN1A Variants?
Authors: Azzarà Alessia , Cassano Ilaria , Lintas Carla , Pilato Fabio , Capone Fioravante , Di Lazzaro Vincenzo , Gurrieri Fiorella
Published: July 2023

Peripheral facial palsy rarely occurs as part of Melkersson–Rosenthal syndrome (MRS), which is characterized by the classical triad of tongue cheilitis, recurrent episodes of orofacial swelling, and palsy. MRS is a disorder with variable expressivity and clinical as well as genetic heterogeneity; however, the causative gene remains to be identified. Migraine is a common neurological disorder, presenting with or without aura, which may be associated with neurological symptoms. The classical example of monogenic migraine is familial hemiplegic migraine (FHM), which has phenotypic variability in carriers of variants in the same gene or even carriers of the same variant. We present a family in which two sisters displayed recurrent migraines, one of which presented recurrent facial palsy and had clinical diagnosis of MRS. We performed WES and Sanger sequencing for segregation analysis in the available family members. We identified a c.3521C>G missense heterozygous variant in SCN1A carried only by the affected sister. Variants in the SCN1A gene can cause a spectrum of early-onset epileptic encephalopathies, in addition to FHM; therefore, our finding reasonably explains the proband phenotype, in which the main symptom was recurrent facial palsy. This report also adds knowledge to the clinical spectrum of SCN1A alterations and suggests a potential overlap between MRS and FHM.
Extracellular Heat Shock Protein 70 Increases the Glucocorticoid Receptor and Dual-Specificity Phosphatase 1 via Toll-like Receptor 4 and Attenuates Inflammation in Airway Epithelial Cells
Authors: Zhou Liang , Fang Lei , Tamm Michael , Stolz Daiana , Roth Michael
Published: July 2023

Heat shock protein 70 (HSP70) regulates the ligand binding of the glucocorticoid receptor (GR). In asthma patients, heat treatment increased both the GR expression and secretion of extracellular HSP70 (eHSP70) by bronchial epithelial cells (EC). The objective of this study was to assess the effects of eHSP70 on GR expression and the GR-dependent regulation of immune response in human bronchial ECs. Cells were treated with either eHSP70 or transfected with an expression vector for intracellular HSP70 (iHSP70). Ribonucleic acid (RNA) and protein levels were detected by reverse transcriptase-polymerase chain reaction (RT-PCR), Western blotting, and immunofluorescence. Interleukin (IL-6 and IL-8) secretion was determined by enzyme linked immunosorbent assay (ELISA). The overexpression of iHSP70 decreased, while eHSP70 increased GR expression. In addition, eHSP70 increased the expression of the GR target dual-specificity phosphatase 1 (DUSP-1). In doing so, eHSP70 reduced the tumor growth factor (TGF)-β1-dependent activation of extracellular signal-regulated kinase (Erk)-1/2 and cyclic AMP response element binding protein (CREB) and the secretion of IL-6 and IL-8. Blocking the GR or Toll-like receptor 4 (TLR4) counteracted all eHSP70-induced effects. This study demonstrates a novel anti-inflammatory effect of eHSP70 by the signaling cascade of TLR4-GR-DUSP1, which inhibits TGF-β1-activated pro-inflammatory ERK1/2-CREB signaling and cytokine secretion. The findings suggest that eHSP70 might present a novel non-steroidal therapeutic strategy to control airway inflammation in asthma.
Maternal Chronic Ultrasound Stress Provokes Immune Activation and Behavioral Deficits in the Offspring: A Mouse Model of Neurodevelopmental Pathology
Authors: Pavlov Dmitrii , Gorlova Anna , Haque Abrar , Cavalcante Carlos , Svirin Evgeniy , Burova Alisa , Grigorieva Elizaveta , Sheveleva Elizaveta , Malin Dmitry , Efimochkina Sofia , Proshin Andrey , Umriukhin Aleksei , Morozov Sergey , Strekalova Tatyana
Published: July 2023

Neurodevelopmental disorders stemming from maternal immune activation can significantly affect a child’s life. A major limitation in pre-clinical studies is the scarcity of valid animal models that accurately mimic these challenges. Among the available models, administration of lipopolysaccharide (LPS) to pregnant females is a widely used paradigm. Previous studies have reported that a model of ‘emotional stress’, involving chronic exposure of rodents to ultrasonic frequencies, induces neuroinflammation, aberrant neuroplasticity, and behavioral deficits. In this study, we explored whether this model is a suitable paradigm for maternal stress and promotes neurodevelopmental abnormalities in the offspring of stressed females. Pregnant dams were exposed to ultrasound stress for 21 days. A separate group was injected with LPS on embryonic days E11.5 and E12.5 to mimic prenatal infection. The behavior of the dams and their female offspring was assessed using the sucrose test, open field test, and elevated plus maze. Additionally, the three-chamber sociability test and Barnes maze were used in the offspring groups. ELISA and qPCR were used to examine pro-inflammatory changes in the blood and hippocampus of adult females. Ultrasound-exposed adult females developed a depressive-like syndrome, hippocampal overexpression of GSK-3β, IL-1β, and IL-6 and increased serum concentrations of IL-1β, IL-6, IL-17, RANTES, and TNFα. The female offspring also displayed depressive-like behavior, as well as cognitive deficits. These abnormalities were comparable to the behavioral changes induced by LPS. The ultrasound stress model can be a promising animal paradigm of neurodevelopmental pathology associated with prenatal ‘emotional stress’.
BASIDIN as a New Protein Effector of the Phytopathogen Causing Witche’s Broom Disease in Cocoa
Authors: Farias Keilane Silva , Ferreira Monaliza Macêdo , Amaral Geiseane Veloso , Zugaib Maria , Santos Ariana Silva , Gomes Fábio Pinto , Rezende Rachel Passos , Gramacho Karina Peres , Aguiar Eric Roberto Guimarães Rocha , Pirovani Carlos Priminho
Published: July 2023

The fungus Moniliophthora perniciosa secretes protein effectors that manipulate the physiology of the host plant, but few effectors of this fungus have had their functions confirmed. We performed functional characterization of a promising candidate effector of M. perniciosa. The inoculation of rBASIDIN at 4 µmol L−1 in the mesophyll of leaflets of Solanum lycopersicum caused symptoms of shriveling within 6 h without the presence of necrosis. However, when sprayed on the plant at a concentration of 11 µmol L−1, it caused wilting symptoms only 2 h after application, followed by necrosis and cell death at 48 h. rBASIDIN applied to Theobroma cacao leaves at the same concentration caused milder symptoms. rBASIDIN caused hydrogen peroxide production in leaf tissue, damaging the leaf membrane and negatively affecting the photosynthetic rate of Solanum lycopersicum plants. Phylogenetic analysis indicated that BASIDIN has orthologs in other phytopathogenic basidiomycetes. Analysis of the transcripts revealed that BASIDIN and its orthologs are expressed in different fungal species, suggesting that this protein is differentially regulated in these basidiomycetes. Therefore, the results of applying BASIDIN allow the inference that it is an effector of the fungus M. perniciosa, with a strong potential to interfere in the defense system of the host plant.
A Commemorative Issue in Honor of 200th Anniversary of the Birth of Gregor Johann Mendel: The Genius of Genetics
Authors: Smýkal Petr , von Wettberg Eric J. B.
Published: July 2023

In celebration of the bicentennial of the birth of Gregor Johann Mendel, the genius of genetics, this Special Issue presents seven papers [...]
Editorial of Special Issue: The Toxicity of Nanomaterials and Legacy Contaminants: Risks to the Environment and Human Health
Authors: Reis Ana Teresa , Costa Carla , Fraga Sónia
Published: July 2023

Nanotechnology and the incorporation of nanomaterials (NM) into everyday products help to solve problems in society and improve the quality of life, allowing for major advances in the technological, industrial, and medical fields [...]
The Effect of Xanthohumol Derivatives on Apoptosis Induction in Canine Lymphoma and Leukemia Cell Lines
Authors: Grudzień Małgorzata , Pawlak Aleksandra , Tronina Tomasz , Kutkowska Justyna , Kruszyńska Angelika , Popłoński Jarosław , Huszcza Ewa , Rapak Andrzej
Published: July 2023

Xanthohumol is a cancer chemopreventive agent that can interfere with the initiation, promotion, and progression phase of carcinogenesis via a variety of inhibitory mechanisms. Xanthohumol was reported as an effective agent against leukemia/lymphoma cells. In the present study, we investigated the effect of xanthohumol and its natural and semisynthetic derivatives against various canine leukemia/lymphoma cell lines. Xanthohumol, three hops minor prenylflavonoids (xanthohumol C, xanthohumol D, α,β-dihydroxanthohumol) and four derivatives obtained by biotransformation (xanthohumol 4′-O-β-D-(4‴-O-methyl)-glucopyranoside) as well as by chemical modification (1″,2″-dihydroxanthohumol K, 2,3-dehydroisoxanthohumol, (Z)-6,4′-dihydroxy-4-methoxy-7-prenylaurone) were tested for their antiproliferative and pro-apoptotic activities against the following canine leukemia/lymphoma cell lines: CLBL-1 (B-cell lymphoma), CLB70 (B-cell leukemia), and GL-1 (B-cell leukemia). The compounds were tested at a final concentration range of 0.1–30 µM for 48 h. All eight of the tested flavonoids exerted concentration-dependent cytotoxicity in the selected canine lymphoma/leukemia cell lines. Three compounds markedly decreased the viability of all cell lines with IC50 in the range of 0.5 to 8 μM. Double-staining of the treated cells with AnnexinV and propidium iodide revealed that the dying cells were mostly in the late apoptosis stage. ROS production and changes in mitochondrial potential were detected. Western blot analysis showed a decreased expression of Bcl-2. Canine lymphoma and leukemia cell lines are sensitive to xanthohumol derivatives, and the compounds acted through an apoptotic cell-death mechanism. These compounds, either used alone or in combination with other therapies, may be useful for the treatment of canine leukemia/lymphoma.
Untargeted Lipidomics after D2O Administration Reveals the Turnover Rate of Individual Lipids in Various Organs of Living Organisms
Authors: Kostyukevich Yury , Stekolshikova Elena , Levashova Anna , Kovalenko Anna , Vishnevskaya Anna , Bashilov Anton , Kireev Albert , Tupertsev Boris , Rumiantseva Lidiia , Khaitovich Philipp , Osipenko Sergey , Nikolaev Eugene
Published: July 2023

The administration of low doses of D2O to living organisms was used for decades for the investigation of metabolic pathways and for the measurement of the turnover rate for specific compounds. Usually, the investigation of the deuterium uptake in lipids is performed by measuring the deuteration level of the palmitic acid residue using GC-MS instruments, and to our knowledge, the application of the modern untargeted LC-MS/MS lipidomics approaches was only reported a few times. Here, we investigated the deuterium uptake for >500 lipids for 13 organs and body liquids of mice (brain, lung, heart, liver, kidney, spleen, plasma, urine, etc.) after 4 days of 100% D2O administration. The maximum deuteration level was observed in the liver, plasma, and lung, while in the brain and heart, the deuteration level was lower. Using MS/MS, we demonstrated the incorporation of deuterium in palmitic and stearic fragments in lipids (PC, PE, TAG, PG, etc.) but not in the corresponding free forms. Our results were analyzed based on the metabolic pathways of lipids.
Calpain Regulation and Dysregulation—Its Effects on the Intercalated Disk
Authors: Yoder Micah W. , Wright Nathan T. , Borzok Maegen A.
Published: July 2023

The intercalated disk is a cardiac specific structure composed of three main protein complexes—adherens junctions, desmosomes, and gap junctions—that work in concert to provide mechanical stability and electrical synchronization to the heart. Each substructure is regulated through a variety of mechanisms including proteolysis. Calpain proteases, a class of cysteine proteases dependent on calcium for activation, have recently emerged as important regulators of individual intercalated disk components. In this review, we will examine how calcium homeostasis regulates normal calpain function. We will also explore how calpains modulate gap junctions, desmosomes, and adherens junctions activity by targeting specific proteins, and describe the molecular mechanisms of how calpain dysregulation leads to structural and signaling defects within the heart. We will then examine how changes in calpain activity affects cardiomyocytes, and how such changes underlie various heart diseases.
Sex-Specific Protection of Endothelial Function after Vascular Ischemia/Reperfusion Injury by the Senomorphic Agent Ruxolitinib
Authors: Saemann Lars , Naujoks Paula , Hartrumpf Lotta , Pohl Sabine , Simm Andreas , Szabó Gábor
Published: July 2023

Ischemia/reperfusion (I/R)-induced endothelial dysfunction occurs in various cardiovascular disorders. I/R injury is partially driven by the release of cytokines. Known for its use in senotherapy, the JAK inhibitor ruxolitinib is able to block the release of cytokines. We investigated the effect of ruxolitinib on the cytokine release and endothelial-dependent vasorelaxation in an in vitro model of I/R. Aortic segments of C57BL/6J mice (N = 12/group) were divided into three groups: control, in vitro I/R (I/R group), and in vitro I/R with ruxolitinib during ischemic incubation (I/R+Ruxo group). We determined cytokine expression. In organ bath chambers, we investigated the maximal endothelial-dependent relaxation to acetylcholine (RmaxACh) and maximal endothelial-independent relaxation to sodium-nitroprusside (RmaxSNP). RmaxACh was decreased in I/R compared to the control (83.6 ± 2.4 vs. 48.6 ± 3.4%; p < 0.05) and I/R+Ruxo (74.4 ± 2.6 vs. 48.6 ± 3.4%; p < 0.05). RmaxSNP was comparable between all groups. IL-10 was detectable only in I/R+Ruxo. CXCL5, CCL2, CCL3, CCL8, CCL11, ICAM-1, IL-1α, IL-7, TNF-α, and G-CSF were decreased or not detectable in I/R+Ruxo. In I/R+Ruxo, ICAM-1 was reduced in rings only from male mice. Treatment of the aorta from mice during in vitro ischemia with the senomorphic agent ruxolitinib reduces cytokine release and protects the endothelium from I/R-mediated dysfunction.
Comparing the Performance of Historical and Regular Stone Pavement Structures in Urban Trafficked Areas through the Finite Element Method (FEM)
Authors: Fiorentini Nicholas , Huang Jiandong , Cuciniello Giacomo , Leandri Pietro , Losa Massimo
Published: July 2023

Stone pavement structures (SPS), also known as stone roads or stone-paved roads, are road pavements constructed using stones as the primary surface material. Different types of SPS exist; historically, irregular-shaped stones with downward protrusions have been often exploited since regular-shaped stones were difficult to be produced. More recently, regular cuboid stones can be also used. Accordingly, in new construction and renovations of SPS, pavement designers must take an essential decision concerning the adoption of historical or regular stones. Nonetheless, it is often confusing which of the two types of stones should be employed, considering that historical and regular SPS follow the same theory and pavement design methods. Therefore, a comparison between the performance of these two types of SPS is required to support their design and maintenance. Moreover, SPS are limitedly investigated and, to the best of our knowledge, there are no research contributions that address this specific task. Accordingly, in the present study, after conducting a laboratory characterization and in situ structural survey by Falling Weight Deflectometer (FWD) on a SPS, a comparative analysis based on the Finite Element Method (FEM) was carried out for investigating the structural performance of the historical (H-SPS) and regular SPS (R-SPS) in urban trafficked areas, where SPS must withstand heavy traffic loads. Specifically, considering both typologies of SPS, the paper aims to model and investigate: (a) the mechanical behavior under loading (displacements, stress, and strain distribution), (b) failure criteria (stone warpage and separation between the stones and the mortar joint), (c) the joint efficiency between stones, and (d) to which extent the road subgrade stiffness may influence the performance of SPS. In addition to the pavement design perspective, the research also provides a short glance at the strengths and weaknesses of R-SPS and H-SPS from other sides, such as functionality, ease of maintenance, construction techniques, and cultural and historical values.
Pituitary Diseases and COVID-19 Outcomes in South Korea: A Nationwide Cohort Study
Authors: Ha Jeonghoon , Kim Kyoung Min , Lim Dong-Jun , Song Keeho , Seo Gi Hyeon
Published: July 2023

The pituitary gland is either directly or indirectly impacted by SARS-CoV-2 infection. As a consequence of SARS-CoV-2 infection, hypothalamic–pituitary dysfunction or pituitary apoplexy can occur. This study aimed to investigate severe COVID-19 outcomes and COVID-19-related mortality in patients with underlying pituitary disease in Korea using a nationwide cohort database. The data required for this study were obtained from the Health Insurance Review and Assessment Service of Korea. Patients with SARS-CoV-2 infection between January 2020 and December 2021 were divided into the following three groups and analyzed: Group A, those who were hospitalized for SARS-CoV-2 infection without underlying pituitary disease (n = 725,170); Group B, those who were hospitalized for SARS-CoV-2 infection with underlying pituitary disease without exposure to systemic steroids (n = 1509); and Group C, patients with underlying pituitary disease and exposure to systemic steroids (n = 365). Differences in severe COVID-19, requirement for oxygen therapy, intensive care unit admission, application of invasive ventilation or use of extracorporeal membrane oxygenation, and COVID-19-related deaths between groups were then analyzed. Group C had the highest rates of hospitalization after COVID-19 infection (82.2%) and mortality within 30 days of infection (6.8%). Group B had a 1.3-fold increase in severe COVID-19 outcomes compared to Group A. Group C had 1.8-fold and 1.3-fold increases in severe COVID-19 outcomes compared to Group A and Group B, respectively. Group C also had 2.34 and 3.24 times higher mortality rates within 30 days of COVID-19 infection than Group A and Group B, respectively. In conclusion, patients with pituitary disease who are receiving systemic steroids have poorer outcomes and higher mortality associated with COVID-19. Therefore, thorough COVID-19 infection control is required in these patients.
Association of Glaucoma with the Risk of Peripheral Arterial Occlusive Disease: A Retrospective Population-Based Cohort Study
Authors: Yeh Han-Wei , Chung Chi-Tzu , Chang Chao-Kai , Yeh Chao-Bin , Wang Bo-Yuan , Lee Chia-Yi , Wang Yu-Hsun , Yeh Liang-Tsai , Yang Shun-Fa
Published: July 2023

This study aimed to investigate the potential association between glaucoma and peripheral arterial occlusive disease. The study recruited patients, including 101,309 with glaucoma and 1,860,528 without a glaucoma diagnosis, from a population of 2 million patients in the Longitudinal Health Insurance Database. Propensity score matching was performed between the two groups, matching for age, sex, and comorbidities. In total, 95,575 patients with glaucoma and 95,575 patients without glaucoma were analyzed for their risk of developing peripheral arterial occlusive disease. The analysis of the data revealed that the glaucoma group had a higher incidence density (ID = 4.13) of peripheral arterial occlusive disease than the non-glaucoma group (ID = 3.42). The relative risk for the glaucoma group was 1.21 (95% C.I. = 1.15–1.28). Cox proportional hazard model analysis indicated that the glaucoma group had a higher risk of developing peripheral arterial occlusive disease (HR = 1.18; 95% C.I. = 1.12–1.25). The subgroup analysis of the risk of PAOD showed that the glaucoma group had a higher risk of developing peripheral arterial occlusive disease in the age group of 20 to 39 (p for interaction = 0.002). In conclusion, patients with glaucoma were associated with a higher risk of subsequent peripheral arterial occlusive disease compared with those without a diagnosis of glaucoma.
Dysfunction of Biliary Sphincter of Oddi—Clinical, Diagnostic and Treatment Challenges
Authors: Kegnæs Marina , Novovic Srdan , Shabanzadeh Daniel Mønsted
Published: July 2023

Biliary Sphincter of Oddi dysfunction (SOD) is one of the main causes of post-cholecystectomy pain. In this review, we aimed to provide an update on the current knowledge on biliary SOD, with an emphasis on diagnostics and therapy. Overall, current but scarce data support biliary sphincterotomy for patients with type 1 and 2 SOD, but not for type 3. However, sphincterotomy is associated with post-treatment pancreatitis rates of from 10% to 15%, thus calling for improved diagnostics, patient selection and treatment modalities for SOD. The role of pharmacologic therapy for patients with SOD is poorly explored and only two randomized controlled trials are available. Currently, studies comparing treatment outcomes are few. There is an unmet need for randomized sham/placebo-controlled clinical trials related to both pharmacological and non-pharmacological treatments of SOD.
The Croatian Primary Sjögren’s Disease Oral Health Study: Oral Status and Oral Health-Related Quality of Life
Authors: Glavina Ana , Božić Ivona , Parat Katica , Perković Dijana , Biočina-Lukenda Dolores , Martinović Kaliterna Dušanka , Radić Mislav
Published: July 2023

To determine salivary flow rate, oral and periodontal status, oral health-related quality of life (OHRQoL), objective and subjective indexes, and serum antibody reactivity in patients with primary Sjögren’s disease (pSD). Thirty-one patients with pSD and 31 control subjects participated in this cross-sectional, single-center study. The unstimulated whole salivary flow rate (UWSFR) and stimulated whole salivary flow rate (SWSFR), salivary pH, DMFT index (DMFT = D—decayed, M—missing, F—filled tooth), periodontal pocket depth (PPD), clinical attachment level (CAL), interincisal distance, OHRQoL, objective European League Against Rheumatism (EULAR) SS Disease Activity Index (ESSDAI) and subjective (EULAR SS Patient Reported Index (ESSPRI), 6-items-VAS-SS (Visual Analog Scale), Profile of Fatigue) indexes were analyzed. The patients with pSD had a blood sample taken in the morning between 7 and 10 a.m. for comprehensive laboratory analysis. Patients with pSD had statistically significant lower UWSFR (0.20 vs. 0.90 mL/min) and SWSFR (0.56 vs. 1.64 mL/min) values compared with control subjects (p < 0.001, Mann-Withney U test). Salivary pH value of pSD patients was significantly lower compared with control subjects (6.00 vs. 7.00; p < 0.001, Mann-Whitney U test). The mean DMFT index of patients with pSD compared to control subjects was not statistically significant (23.74 ± 7.28 vs. 20.77 ± 5.73; p = 0.08, t-test). Interincisal distance was significantly decreased in the pSD group compared with control subjects (43.80 ± 0.38 vs. 47.60 ± 0.50; p = 0.003, t-test). The prevalence of periodontitis was similar in patients with pSD and control subjects (83.9% vs. 77.4%; p = 0.35, λ2 test). The mean Oral Health Impact Profile (OHIP-49) total score was statistically significantly higher in pSD patients compared with control subjects (32.00 vs. 8.00; p < 0.001, Mann–Whitney U test). Patients with pSD have decreased salivary flow and salivary pH, poor oral health, decreased interincisal distance, high prevalence of periodontitis, and worse OHRQoL. These findings highlight the need for a multidisciplinary approach to the management of patients with pSD that includes physical and psychological aspects of the disease.
Strength of Ship Structures
Authors: Oterkus Erkan
Published: July 2023

Ships are the main drivers of global transportation [...]
A Single Intradermal Injection of Autologous Adipose-Tissue-Derived Stem Cells Rejuvenates Aged Skin and Sharpens Double Eyelids
Authors: Ichihashi Masamitsu , Tanaka Masaki , Iizuka Takashi , Totsuka Hiroko , Tominaga Ekuko , Hitomi Yuka , Ando Hideya , Nishikata Takahiro , Mizutani Ken-Ichi
Published: July 2023

Facial skin aging is the most visible manifestation of aging in the body. In this study, we aimed to rejuvenate aging skin via a one-time intradermal injection of autologous adipose-derived stem cells (ADSCs). Eight patients were enrolled for study. Photographs of patients taken immediately before and 1, 3, 6, and 12 months after ADSC injections were comparatively evaluated for visible skin manifestations. ADSCs were cultured from the abdominal-skin-derived subcutaneous fat tissue, and 1 × 108 cultured ADSCs were injected intradermally into the facial skin. Cultured myoblasts were incubated with the supernatant derived from ADSCs, and the effect was evaluated via glucose consumption and lactic acid production in the medium. Eight cases showed the shallowing and disappearance of wrinkles, including those of the glabella, lower eyelids, crow`s feet, and forehead and nasolabial grooves, a month to several months after treatment. Double eyelids became prominent, and facial pores significantly reduced in size. These effects lasted for over one year. Myoblasts cultured in the presence of an ADSC-derived exosome were activated compared to that of ADSCs cultured without supernatant. The result supports the role of muscle in ADSC skin rejuvenation. The present study first reports that a single intradermal administration of cultured ADSCs rejuvenates aged facial skin over the course of one year. Further, patients exhibited definite double eyelids and pore shrinkage, strongly indicating the active involvement of muscle, which was supported by an in vitro study. Our study also suggested the important role of biological factors delivered from injected stem cells, although the detailed mechanism of rejuvenation effects of ADSC skin injection remains to be clarified.
A Validation Study of cT-Categories in the Swedish National Urinary Bladder Cancer Register—Norrland University Hospital
Authors: Wiberg Erik , Vega Andrés , Eriksson Victoria , Banday Viqar , Svensson Johan , Eriksson Elisabeth , Jahnson Staffan , Sherif Amir
Published: July 2023

Background: In Sweden, all patients with urinary bladder cancer (UBC) are recorded in the Swedish National Register for Urinary Bladder Cancer (SNRUBC). The purpose of this study was to validate the registered clinical tumour categories (cT-categories) in the SNRUBC for Norrland University Hospital, Sweden, from 2009 to 2020, inclusive. Methods: The medical records of all 295 patients who underwent radical cystectomy for the treatment of UBC were reviewed retrospectively. Possible factors impacting the cT-categories were identified. To optimise cT-classification, computed tomography urography of all patients with suspected tumour-associated hydronephrosis (TAH) or suspected tumour in bladder diverticulum (TIBD) were retrospectively reviewed by a radiologist. Discrepancy was tested with a logistic regression model. Results: cT-categories differed in 87 cases (29.5%). Adjusted logistic regression analysis found TIBD and TAH as significant predictors for incorrect registration; OR = 7.71 (p < 0.001), and OR = 17.7, (p < 0.001), respectively. In total, 48 patients (68.6%) with TAH and 12 patients (52.2%) with TIBD showed discrepancy regarding the cT-category. Incorrect registration was mostly observed during the years 2009–2012. Conclusion: The study revealed substantial incorrect registration of cT-categories in SNRUBC. A major part of the misclassifications was related to TAH and TIBD. Registration of these variables in the SNRUBC might be considered to improve correct cT-classification.
Genetic Structure and Biodiversity in Wild Centropomus parallelus and in Wild and Recently Domesticated Centropomus undecimallis Populations
Authors: Herkenhoff Marcos Edgar , Bajay Miklos Maximiliano , Costamilan Carlos André da Veiga Lima-Rosa
Published: July 2023

Centropomus undecimalis (common snook) and Centropomus parallelus (fat snook) have a wide distribution from southern Florida to southern Brazil. Due to their value as a food source, these species have been heavily exploited through predatory fishing, posing a conservation challenge. To assess their genetic diversity and population structure, we used microsatellite markers. Our findings revealed genetic differences among populations of the same species, highlighting the need for targeted conservation efforts. The microsatellite markers proved effective in assessing genetic variability, providing valuable insights for management and conservation. The parameters Ho (observed heterozygosity) and He (expected heterozygosity) were reliable indicators of genetic diversity, and specific loci showed varying allele numbers across populations. Our study contributes to understanding population genetics in these snook species and supports their conservation. Despite not being classified as endangered, genetic differences among populations emphasize the importance of considering population-level characteristics in conservation strategies. This research lays the foundation for future studies and actions aimed at preserving these valuable fish species. In summary, our study demonstrates the significance of microsatellite markers in assessing genetic variability and population structure in common snook and fat snook, informing conservation efforts for these species.
What Makes a Good Plant Invader?
Authors: Korpelainen Helena , Pietiläinen Maria
Published: July 2023

We explored traits that promote plant invasions. External factors affecting invasion success consist of various abiotic and biotic constraints. How well plants perform under those depends on multiple characteristics, such as life history traits, genetic variation patterns, competitive and dispersal abilities, phenotypic plasticity, resistance, tolerance, and possibly allelopathic interactions. Since the introduction of invasive species is often connected with humans, their geographical distribution and differentiation may not reflect adaptation. However, a lack of adaptation may be compensated for by repeated introductions via mixing genotypes from multiple populations or through novel mutations. As a case study, we used data from the Global Invasive Species Database of IUCN and attempted to reveal factors contributing to invasiveness. The most prevalent features are that the dispersal is strongly human assisted, many species are used as ornamentals, disturbed habitats are favored, and most species are perennial. Distribution features show that the worst invasive species typically have a narrower native distribution, but both groups, i.e., most serious invasive and other listed invasive species, have commonly developed a multicontinental distribution. The change in the multicontinental distribution from 6% to 63% in most serious invasive species reflects their effectiveness in global dispersal and establishment. High proportions of invasive species in both groups have mixed reproduction systems, i.e., they have the ability to propagate both sexually and asexually (57% and 50%, respectively). This provides flexibility for spreading and establishment. A lower proportion of the worst invasive species was mainly/only sexual (23%, often hermaphrodites) when compared to other invasive plants (40%). In the case of sexual reproduction, hermaphroditism combined with self-compatibility may enhance invasiveness, since selfing allows fertilization and recombination even under low population densities. Overall, the ability for asexual propagation and, in the case of sexuality, hermaphroditism, is an asset in the invasion process.
Effects of Variated Final Temperature and Workpiece Thickness for Hot Rolling of Aluminum Alloy EN AW-8011
Authors: Kraner Jakob , Cvahte Peter , Šuštarič Primož , Šuštar Tomaž , Donik Črtomir , Paulin Irena , Kim Shae K. , Kim Kyung Il
Published: July 2023

Hot rolling in the process chain of aluminum-rolled products presents the critical element of material quality and influences productivity. To increase the letter demand modifications of hot rolling, the consequential changes of microstructure, crystallographic texture, and mechanical and formability properties must be acknowledged and consistently considered when planning the rolling process and rolled product. Achieving lower thicknesses of the hot-rolled band would enable fewer passes with cold rolling; consequently, hot rolling with the same number of passes can be completed with lower temperatures. Microstructural and texture characterizations conducted using the light microscope and scanning electron microscope, respectively, of the 3.25 mm hot-rolled band revealed that the smaller grains appeared in the center of the cross-section, unlike for the 6 mm hot-rolled band, where smaller grains were detected on the top and bottom positions of the cross-section. Furthermore, the comparison also shows that the 6 mm hot-rolled band had 64% of random texture components and 83% of recrystallized grains, whereas the proportional adjustment for the 3.25 mm hot-rolled band had 42% of random texture components and 55% of recrystallized grains. For the mechanical testing results, the elongation values in rolling and transverse directions significantly differ only in the case of a hot-rolled band of 3.25 mm. Consequently, the earing results are more than 1.5% higher for the 3.25 mm hot-rolled band, than the 6 mm hot-rolled band.
The Phase Transformation in a Low-Carbon 13Cr4Ni Martensitic Stainless Steel during Two-Stage Intercritical Tempering
Authors: He Zhiyang , Wang Pei , Liu Gongmei , Liu Jie , Zhang Shenghua
Published: July 2023

The microstructure evolution of a low-carbon 13Cr4Ni martensitic stainless steel during two-stage intercritical tempering at 630 °C and 590 °C has been investigated by X-ray diffraction, thermodilatometry, and transmission electron microscopy. It was found that the amount and size of reversed austenite increase remarkably after second-stage tempering at 590 °C. However, there is no remarkable variation in the chemical composition and nucleation site of the reversed austenite during the first- and second-stage tempering. The dynamics of the phase transformation and elements distribution imply that the martensite-to-austenite phase transformation during second-stage tempering is controlled by diffusion. The unstable austenite transformation into martensite during the cooling process of the first-stage tempering induces high density dislocations and inhomogeneous element distribution, which facilitate the nucleation and growth of the reversed austenite in the second-stage intercritical tempering. Additionally, some lathy reversed austenite spheroidizes to granular during second-stage tempering.
Metabolomics-Based Investigation on the Metabolic Changes in Crassostrea gigas Experimentally Exposed to Galvanic Anodes
Authors: Imbert-Auvray Nathalie , Fichet Denis , Bodet Pierre-Edouard , Ory Pascaline , Sabot René , Refait Philippe , Graber Marianne
Published: July 2023

Cathodic protection is widely used to protect metal structures from corrosion in marine environments using sacrificial galvanic anodes. These anodes, either in Zinc, or preferentially nowadays in Al-Zn-In alloys, are expected to corrode instead of the metal structures. This leads to the release of dissolved species, Zn2+, Al3+, and In3+, and solid phases such as Al(OH)3. Few studies have been conducted on their effects on marine organisms, and they concluded that further investigations are needed. We therefore evaluated the effects of Zn and Al-Zn-In anodes on oysters stabulated in tanks, under controlled conditions defined through a comparison with those prevailing in a given commercial seaport used as reference. We analyzed the entire metabolome of gills with a non-targeted metabolomic approach HRMS. A modelling study of the chemical species, corresponding to the degradation products of the anodes, likely to be present near the exposed oysters, was also included. We identified 16 and two metabolites modulated by Zn- and Al-Zn-In-anodes, respectively, that were involved in energy metabolism, osmoregulation, oxidative stress, lipid, nucleotide nucleoside and amino acid metabolisms, defense and signaling pathways. The combination of chemical modelling and metabolomic approach, used here for the first time, enlightened the influence of Zn present in the Al-Zn-In anodes.
Role of Bubble Evolution in the Bubble-Propelled Janus Micromotors
Authors: Chen Gang , Wang Xuekui , Zhang Bingyang , Zhang Fangfang , Wang Zhibin , Zhang Baiqiang , Li Guopei
Published: July 2023

Bubble-propelled Janus micromotors have attracted extensive attention in recent years and have been regarded as powerful tools in the environmental and medical fields due to their excellent movement ability. The movement ability can mainly be attributed to the periodic growth, detachment, and/or collapse of the bubble. However, subjected to the experimental conditions, the mechanism of bubble evolution on the motion of the micromotor could not be elucidated clearly. In this work, a finite element method was employed for exploring the role of bubble evolution in bubble-propelled Janus micromotors, which emphasized the growth and collapse of bubbles. After the proposed model was verified by the scallop theorem, the influence of the growth and rapid collapse of bubbles on micromotors was investigated. Results show that the growth and collapse of a bubble can drive the micromotor to produce a displacement, but the displacement caused by a bubble collapse is significantly greater than that caused by bubble growth. The reasons for this phenomenon are analyzed and explained. In addition to the influence of bubble size, the collapse time of the bubble is also investigated.
Effect of P-Type GaN Buried Layer on the Temperature of AlGaN/GaN HEMTs
Authors: Lv Hanghang , Cao Yanrong , Ma Maodan , Wang Zhiheng , Zhang Xinxiang , Chen Chuan , Wu Linshan , Lv Ling , Zheng Xuefeng , Wang Yongkun , Tian Wenchao , Ma Xiaohua
Published: July 2023

In this paper, a P-type GaN buried layer is introduced into the buffer layer of AlGaN/GaN HEMTs, and the effect of the P-type GaN buried layer on the device’s temperature characteristics is studied using Silvaco TCAD software. The results show that, compared to the conventional device structure, the introduction of a P-type GaN buried layer greatly weakens the peak of the channel electric field between the gate and drain of the device. This leads to a more uniform electric field distribution, a substantial reduction in the lattice temperature of the device, and a more uniform temperature distribution. Therefore, the phenomenon of negative resistance caused by self-heating effect is significantly mitigated, while the breakdown performance of the device is also notably enhanced.