bluuebunny/crossref_metadata_2025 huggingface dataset:
HILIC-MS for Untargeted Profiling of the Free Glycation Product Diversity
Authors: Yan Yingfei , Hemmler Daniel , Schmitt-Kopplin Philippe
Published: November 2022

Glycation products produced by the non-enzymatic reaction between reducing carbohydrates and amino compounds have received increasing attention in both food- and health-related research. Although liquid chromatography mass spectrometry (LC-MS) methods for analyzing glycation products already exist, only a few common advanced glycation end products (AGEs) are usually covered by quantitative methods. Untargeted methods for comprehensively analyzing glycation products are still lacking. The aim of this study was to establish a method for simultaneously characterizing a wide range of free glycation products using the untargeted metabolomics approach. In this study, Maillard model systems consisting of a multitude of heterogeneous free glycation products were chosen for systematic method optimization, rather than using a limited number of standard compounds. Three types of hydrophilic interaction liquid chromatography (HILIC) columns (zwitterionic, bare silica, and amide) were tested due to their good retention for polar compounds. The zwitterionic columns showed better performance than the other two types of columns in terms of the detected feature numbers and detected free glycation products. Two zwitterionic columns were selected for further mobile phase optimization. For both columns, the neutral mobile phase provided better peak separation, whereas the acidic condition provided a higher quality of chromatographic peak shapes. The ZIC-cHILIC column operating under acidic conditions offered the best potential to discover glycation products in terms of providing good peak shapes and maintaining comparable compound coverage. Finally, the optimized HILIC-MS method can detect 70% of free glycation product features despite interference from the complex endogenous metabolites from biological matrices, which showed great application potential for glycation research and can help discover new biologically important glycation products.
Short-Term Effects of Spirulina Consumption on Glycemic Responses and Blood Pressure in Healthy Young Adults: Results from Two Randomized Clinical Trials
Authors: Lympaki Foteini , Giannoglou Marianna , Magriplis Emmanuella , Bothou Dionysia Lydia , Andreou Varvara , Dimitriadis George D. , Markou Giorgos , Zampelas Antonis , Theodorou Georgios , Katsaros George , Papakonstantinou Emilia
Published: November 2022

The effects of spirulina consumption added in foods were investigated in two crossover clinical trials (n = 13 different healthy adults). In Trial-1 adults consumed cookies with-and-without spirulina (3.12 g per 100 g final product; 2.5 g spirulina per 50 g available carbohydrates) according to glycemic index (GI) methodology. In Trial-2, adults consumed 4 g, 6 g, and 8 g spirulina as beverage diluted in 50 g D-glucose vs. 50 g plain D-glucose. Capillary blood glucose samples were collected at 0, 15, 30, 45, 60, 90, and 120 min and blood pressure (BP) was measured at beginning and end of each visit in both trials. Trial-1: both cookies with and without spirulina provided medium GI values (59 and 60, respectively, on glucose-scale), but no significant differences were found for BP. Trial-2: both 4 g and 8 g spirulina lowered postprandial glucose at 120 min (95% CI: −1.64 to −16.12 and −1.23 to −15.87, respectively). The results explained 29% of variation. Only 8 g spirulina decreased significantly 90–120 min area under the curve (AUC) for glucose and systolic BP (−4%). No differences were found for fasting glucose. Adding spirulina to cookies did not affect glucose responses and BP. Only 8 g provided significantly lower 90–120 min-AUC for glucose and BP compared to 4 g, 6 g-and-D-glucose, indicating advantages to glycemic control and hypertension.
Impaired Carbohydrate Metabolism and Excess of Lipid Accumulation in Offspring of Hyperandrogenic Mice
Authors: Aburto-Hernández Cynthia , Barrera David , Ortiz-Hernández Rosario , Espinoza-Simón Emilio , Parra-Gámez Leticia , González James , Escobar M Luisa , Vázquez-Nin Gerardo H , Echeverría-Martínez Olga , Torres-Ramírez Nayeli
Published: November 2022

Polycystic ovary syndrome (PCOS) is an endocrine–metabolic disorder of unknown etiology. Hyperandrogenism (HA) is the main diagnostic criteria for PCOS, in addition to being a risk factor for developing several disorders throughout the patient’s life, including pregnancy. However, the impact on offspring is little known. Therefore, the aim of this work was to evaluate the effect of maternal HA on glucose metabolism and hepatic lipid accumulation in adult offspring. We used Balb/c mice treated with dehydroepiandrosterone (DHEA) for 20 consecutive days. The ovary of DHEA-treated mice showed hemorrhagic bodies, an increased number of atretic follicles, and greater expression of genes related to meiotic cell cycle and DNA repair. The DHEA offspring (O-DHEA) had low birth weight, and some pups showed malformations. However, O-DHEA individuals gained weight rapidly, and the differences between them and the control group became significantly greater in adulthood. Moreover, O-DHEA presented higher serum glucose after a 6 h fast and a larger area under glucose, insulin, and pyruvate tolerance test curves. Oil Red O staining showed a more significant accumulation of fat in the liver but no changes in serum cholesterol and triacylglycerol levels. In summary, our results show that HA, induced by DHEA, affects gene expression in oocyte, which in turn generates defects in embryonic development, insulin resistance, and alteration in hepatic gluconeogenesis and lipid metabolism in O-DHEA, thereby increasing the risk of developing metabolic diseases.
Myoinositol vs. Metformin in Women with Polycystic Ovary Syndrome: A Randomized Controlled Clinical Trial
Authors: Ravn Pernille , Gram Freja , Andersen Marianne S. , Glintborg Dorte
Published: November 2022

Polycystic ovary syndrome (PCOS) is associated with insulin resistance. Few randomized controlled trials (RCT) compared myoinositol (MI) with metformin (MET) regarding insulin resistance in PCOS. This was an open-label six-month RCT in women with PCOS (n = 45) with interventions MI 4 g/day or MET 2 g/day. Primary outcome was the homeostasis model assessment of insulin resistance (HOMA-IR). Secondary outcomes were fasting glucose, weight, cycle length, lipids, testosterone, adverse effects, quality of life, and depression scores. Median age was 26 years. Body mass was index was 34.4 kg/m2. HOMA-IR was unchanged during MI (p = 0.31) and MET (p = 0.11) (MI vs. MET, p = 0.09). Median fasting glucose changed +0.2 mmol/L during MI (p < 0.001) and −0.1 mmol/L during MET (p = 0.04) (MI vs. MET p < 0.001). Median weight changed −2.3 kg during MI (p = 0.98) and −6.1 kg during MET (p < 0.001) (MI vs. MET, p = 0.02). Median cycle length decreased nine days during MI (p = 0.03) and 13 days during MET (p = 0.03) (MI vs. MET, p = 0.93). High-density lipoprotein (HDL) changed +0.1 mmol/L during MET (p = 0.04) (MI vs. MET, p = 0.07). All other blood parameters and scores of quality of life and depression remained unchanged during MI and MET (all p > 0.06) (MI vs. MET, all p > 0.27). Adverse effects appeared in four women during MI and 16 women during MET (MI vs. MET, p = 0.001). In conclusion, there was no effect on the metabolic outcomes during MI, but positive effects on fasting blood glucose, weight, and HDL during MET. The effect on cycle length was comparable during MI and MET. Adverse effects were less frequent during MI.
Petrogenesis of Alkaline Complex of the Longbaoshan Rare Earth Element Deposit in the Luxi Block, North China Craton, China
Authors: Yang Ze-Yu , Li Shan-Shan , An Mao-Guo , Zhi Cheng-Long , Shang Zhen , Long Zheng-Yu , Geng Jian-Zhen , Yu Hao-Cheng , Qiu Kun-Feng
Published: November 2022

The alkaline complex in the southwest region of Luxi Terrane of the North China Craton is spatially correlated with the newly discovered Longbaoshan REE deposit. Its petrogenesis, however, remains ambiguous. In this study, we present an integrated petrology, whole-rock geochemistry, sphene U-Pb and rare earth element data from the Longbaoshan alkaline complex to investigate the petrogenesis, magma source and tectonic evolution. The Longbaoshan alkaline complex consists of mafic to intermediate rocks of hornblende diorite and alkaline hornblende syenite porphyry, biotite monzonite porphyry and aegirine diorite porphyrite. The hornblende diorites show a composition of low SiO2, high MgO, Fe2O3 and moderate Na2O, CaO and are metaluminous and medium-to-high-K calc-alkaline. The hornblende syenite porphyries, biotite monzonites and argirine diorite porphyrites display a relatively higher content of SiO2, Na2O, K2O and Al2O3 and lower contents of MgO, Fe2O3 and CaO and are metaluminous, peralkaline, high-K calcic-alkaline and shoshonite. The sphene U-Pb data shows that the parent magma of the hornblende diorite was emplaced at ca. 120 Ma. All these samples show a common depletion in Th, Nb-Ta and Zr-Hf and enrichment in large ion lithophile elements (e.g., Pb, Ba, Sr) and Light Rare Earth Elements. The magma may have experienced fractionation of pyroxene, amphibole, sphene, apatite and zircon during its evolution. The variable La content, La/Sm, Rb/Sr and (Ta/Th) N ratios indicate that the parent magma may produce by partial melting of a mantle source that was interacted with sediment-derived melts in a subduction setting. Therefore, we propose that the parent magma of the Longbaoshan alkaline complex was derived from a lithospheric mantle which was metasomatized by sediment-derived melt in a prior subduction process. The enriched magma was emplaced through an extension process and experienced subsequent fractionation and assimilation with the continental crust during the rollback of the Paleo Pacific Ocean plate.
Carbon Paper Modified with Functionalized Poly(diallyldimethylammonium chloride) Graphene and Gold Phytonanoparticles as a Promising Sensing Material: Characterization and Electroanalysis of Ponceau 4R in Food Samples
Authors: Stozhko Natalia Yu. , Khamzina Ekaterina I. , Bukharinova Maria A. , Tarasov Aleksey V. , Kolotygina Veronika Yu. , Lakiza Natalia V. , Kuznetcova Ekaterina D.
Published: November 2022

This paper presents a novel eco-friendly sensing material based on carbon paper (CP) volumetrically modified with a composite nanomodifier that includes functionalized poly(diallyldimethylammonium chloride) graphene (PDDA-G) and phytosynthesized gold nanoparticles (phyto-AuNPs). The functionalization of graphene was justified by Fourier-transform infrared spectroscopy. The phyto-AuNPs (d = 6 nm) were prepared by “green” synthesis with the use of strawberry leaf extract. The sensing material was characterized using scanning electron microscopy, electrochemical impedance spectroscopy, and voltammetry. The research results indicated a more than double increase in the electroactive surface area; a decrease in the resistance of electron transfer on nanocomposite-modified CP, compared to bare CP. The phyto-AuNPs/PDDA-G/CP was used for the electrosensing of the synthetic dye Ponceau 4R. The oxidation signal of colorant enhanced 4-fold on phyto-AuNPs/PDDA-G/CP in comparison to CP. The effect of the quantity of nanomodifier, solution pH, potential scan rate, accumulation parameters, and differential pulse parameters on the peak current of Ponceau 4R was studied. Under optimal conditions, excellent sensory characteristics were established: LOD 0.6 nM and LR 0.001–2 μM for Ponceau 4R. High selectivity and sensitivity enable the use of the sensor for analyzing the content of Ponceau 4R in food products (soft drinks, candies, and popsicles) without additional sample preparation.
Digital Twins Solve the Mystery of Raman Spectra of Parental and Reduced Graphene Oxides
Authors: Sheka Elena F.
Published: November 2022

Digital Twins concept presents a new trend in virtual material science, common to all computational techniques. Digital twins, virtual devices and intellectual products, presenting the main constituents of the concept, are considered in detail on the example of a complex problem, which concerns an amazing identity of the D-G-doublet Raman spectra of parental and reduced graphene oxides. Digital twins, presenting different aspects of the GO and rGO structure and properties, were virtually synthesized using a spin-density algorithm emerging from the Hartree-Fock approximation. Virtual device presents AM1 version of the semi-empirical unrestricted HF approximation. The equilibrium structure of the twins as well as virtual one-phonon harmonic spectra of IR absorption and Raman scattering constitute a set of intellectual products. It was established that in both cases the D-G doublets owe their origin to the sp3 and sp2 C-C stretchings, respectively. This outwardly similar community reveals different grounds. Thus, multilayer packing of individual rGO molecules in stacks provides the existence of the sp3 D band in addition to sp2 G one. The latter is related to stretchings of the main pool of sp2 C-C bonds, while the sp3 constituent presents out-of-plane stretchings of dynamically stimulated interlayer bonds. In the GO case, the sp3 D component, corresponding to stretchings of the main pool of sp3 C-C bonds, is accompanied by an sp2 G component, which is related to stretchings of the remaining sp2 C-C bonds provided with the spin-influenced prohibition of the 100% oxidative reaction in graphene domain basal plane.
Carbon-Supported PdCu Alloy as Extraordinary Electrocatalysts for Methanol Electrooxidation in Alkaline Direct Methanol Fuel Cells
Authors: Li Guixian , Wang Shoudeng , Li Hongwei , Guo Peng , Li Yanru , Ji Dong , Zhao Xinhong
Published: November 2022

Palladium (Pd) nanostructures are highly active non-platinum anodic electrocatalysts in alkaline direct methanol fuel cells (DMFCs), and their electrocatalytic performance relies highly on their morphology and composition. This study reports the preparation, characterizations, and electrocatalytic properties of palladium-copper alloys loaded on the carbon support. XC-72 was used as a support, and hydrazine hydrate served as a reducing agent. PdxCuy/XC-72 nanoalloy catalysts were prepared in a one-step chemical reduction process with different ratios of Pd and Cu. A range of analytical techniques was used to characterize the microstructure and electronic properties of the catalysts, including transmission electron microscopy (TEM), X-ray diffractometry (XRD), X-ray photoelectron spectroscopy (XPS), and inductively coupled plasma emission spectroscopy (ICP-OES). Benefiting from excellent electronic structure, Pd3Cu2/XC-72 achieves higher mass activity enhancement and improves durability for MOR. Considering the simple synthesis, excellent activity, and long-term stability, PdxCuy/XC-72 anodic electrocatalysts will be highly promising in alkaline DMFCs.
Formulation, Characterization, Anti-Inflammatory and Cytotoxicity Study of Sesamol-Laden Nanosponges
Authors: Nair Anroop B. , Dalal Pooja , Kadian Varsha , Kumar Sunil , Kapoor Archana , Garg Minakshi , Rao Rekha , Aldhubiab Bandar , Sreeharsha Nagaraja , Almuqbil Rashed M. , Attimarad Mahesh , Elsewedy Heba S. , Shinu Pottathil
Published: November 2022

Sesamol (SES) possesses remarkable chemotherapeutic activity, owing to its anti-inflammatory and antioxidant potential. However, the activity of SES is mainly hampered by its poor physicochemical properties and stability issues. Hence, to improve the efficacy of this natural anti-inflammatory and cytotoxic agent, it was loaded into β-cyclodextrin nanosponges (NS) prepared using different molar ratios of polymer and crosslinker (diphenyl carbonate). The particle size of SES-laden NS (SES-NS) was shown to be in the nano range (200 to 500 nm), with a low polydispersity index, an adequate charge (−17 to −26 mV), and a high payload. Field emission scanning electron microscopy, thermogravimetric analysis, and Fourier transform infrared spectroscopy were used to characterize the bioactive-loaded selected batch (SES-NS6). This batch of nanoformulations showed improved solubilization efficacy (701.88 µg/mL) in comparison to bare SES (244.36 µg/mL), polymer (β-CD) (261.43 µg/mL), and other fabricated batches. The drug release data displayed the controlled release behavior of SES from NS. The findings of the egg albumin denaturation assay revealed the enhanced anti-inflammatory potential of SES-NS as compared to bare SES. Further, the cytotoxicity assay showed that SES-NS was more effective against B16F12 melanoma cell lines than the bioactive alone. The findings of this assay demonstrated a reduction in the IC50 values of SES-NS (67.38 μg/mL) in comparison to SES (106 μg/mL). The present investigation demonstrated the in vitro controlled release pattern and the enhanced anti-inflammatory and cytotoxic activity of SES-NS, suggesting its potential as a promising drug delivery carrier for topical delivery.
Plasma Ag-Modified α-Fe2O3/g-C3N4 Self-Assembled S-Scheme Heterojunctions with Enhanced Photothermal-Photocatalytic-Fenton Performances
Authors: Xiao Yawei , Yao Bo , Wang Zhezhe , Chen Ting , Xiao Xuechun , Wang Yude
Published: November 2022

Low spectral utilization and charge carrier compounding limit the application of photocatalysis in energy conversion and environmental purification, and the rational construction of heterojunction is a promising strategy to break this bottleneck. Herein, we prepared surface-engineered plasma Ag-modified α-Fe2O3/g-C3N4 S-Scheme heterojunction photothermal catalysts by electrostatic self-assembly and light deposition strategy. The local surface plasmon resonance effect induced by Ag nanoparticles broadens the spectral response region and produces significant photothermal effects. The temperature of Ag/α-Fe2O3/g-C3N4 powder is increased to 173 °C with irradiation for 90 s, ~3.2 times higher than that of the original g-C3N4. The formation of 2D/2D structured S-Scheme heterojunction promotes rapid electron-hole transfer and spatial separation. Ternary heterojunction construction leads to significant enhancement of photocatalytic performance of Ag/α-Fe2O3/g-C3N4, the H2 photocatalytic generation rate up to 3125.62 µmol g−1 h−1, which is eight times higher than original g-C3N4, and the photocatalytic degradation rate of tetracycline to reach 93.6%. This thermally assisted photocatalysis strategy improves the spectral utilization of conventional photocatalytic processes and provides new ideas for the practical application of photocatalysis in energy conversion and environmental purification.
Morphology-Controlled Green Synthesis of Magnetic Nanoparticles Using Extracts of ‘Hairy’ Roots: Environmental Application and Toxicity Evaluation
Authors: Kobylinska Natalia , Klymchuk Dmytro , Khaynakova Olena , Duplij Volodymyr , Matvieieva Nadiia
Published: November 2022

Magnetic nanoparticles (MNPs) were “green” synthesized from a FeCl3/FeSO4/CoCl2 mixture using ethanolic extracts of Artemisia tilesii Ledeb ‘hairy’ roots. The effect of chemical composition and reducing power of ethanolic extracts on the morphology, size destribution and other features of obtained MNPs was evaluated. Depending on the extract properties, nanosized magnetic materials of spherical (8–11 nm), nanorod-like (15–24 nm) and cubic (14–24 nm) shapes were obtained via self-assembly. Microspherical MNPs composed of nanoclusters were observed when using extract of the control root line in the synthesis. Polyhedral magnetic nanoparticles with an average size of ~30 nm were formed using ‘hairy’ root ethanolic extract without any additive. Studied samples manifested excellent magnetic characteristics. Field-dependent magnetic measurements of most MNPs demonstrated a saturation magnetization of 42.0–72.9 emu/g with negligible coercivity (∼0.02–0.29 emu/g), indicating superparamagnetic behaviour only for solids with a magnetite phase. The synthesized MNPs were minimally aggregated and well-dispersed in aqueous medium, probably due to their stabilization by bioactive compounds in the initial extract. The nanoparticles were tested for magnetic solid-phase extraction of copper (Cu), cadmium (Cd) and arsenic (As) pollutants in aqueous solution, followed by ICP-OES analysis. The magnetic oxides, mainly magnetite, showed high adsorption capacity and effectively removed arsenic ions at pH 6.7. The maximum adsorption capacity was ~150 mg/g for As(III, V) on the selected MNPs with cubic morphology, which is higher than that of previously reported adsorbents. The best adsorption was achieved using Fe3O4-based nanomaterials with low crystallinity, non-spherical form and a large number of surface-localized organic molecules. The phytotoxicity of the obtained MNPs was estimated in vitro using lettuce and chicory as model plants. The obtained MNPs did not exhibit inhibitory activity. This work provides novel insights on the morphology of “green” synthesized magnetic nanoparticles that can be used for applications in adsorption technologies.
Organic–Inorganic Lead Halide Perovskite Single Crystal: From Synthesis to Applications
Authors: Liang Zhenye , Tian Chen , Li Xiaoxi , Cheng Liwei , Feng Shanglei , Yang Lifeng , Yang Yingguo , Li Lina
Published: November 2022

Organic–inorganic lead halide perovskite is widely used in the photoelectric field due to its excellent photoelectric characteristics. Among them, perovskite single crystals have attracted much attention due to its lower trap density and better carrier transport capacity than their corresponding polycrystalline materials. Owing to these characteristics, perovskite single crystals have been widely used in solar cells, photodetectors, light-emitting diode (LED), and so on, which have greater potential than polycrystals in a series of optoelectronic applications. However, the fabrication of single-crystal devices is limited by size, thickness, and interface problems, which makes the development of single-crystal devices inferior to polycrystalline devices, which also limits their future development. Here, several representative optoelectronic applications of perovskite single crystals are introduced, and some existing problems and challenges are discussed. Finally, we outlook the growth mechanism of single crystals and further the prospects of perovskite single crystals in the further field of microelectronics.
Nipah and Hendra Viruses: Deadly Zoonotic Paramyxoviruses with the Potential to Cause the Next Pandemic
Authors: Gazal Sabahat , Sharma Neelesh , Gazal Sundus , Tikoo Mehak , Shikha Deep , Badroo Gulzar Ahmed , Rashid Mohd , Lee Sung-Jin
Published: November 2022

Nipah and Hendra viruses are deadly zoonotic paramyxoviruses with a case fatality rate of upto 75%. The viruses belong to the genus henipavirus in the family Paramyxoviridae, a family of negative-sense single-stranded RNA viruses. The natural reservoirs of NiV and HeV are bats (flying foxes) in which the virus infection is asymptomatic. The intermediate hosts for NiV and HeV are swine and equine, respectively. In humans, NiV infections result in severe and often fatal respiratory and neurological manifestations. The Nipah virus was first identified in Malaysia and Singapore following an outbreak of encephalitis in pig farmers and subsequent outbreaks have been reported in Bangladesh and India almost every year. Due to its extreme pathogenicity, pandemic potential, and lack of established antiviral therapeutics and vaccines, research on henipaviruses is highly warranted so as to develop antivirals or vaccines that could aid in the prevention and control of future outbreaks.
Drug Repurposing Approaches towards Defeating Multidrug-Resistant Gram-Negative Pathogens: Novel Polymyxin/Non-Antibiotic Combinations
Authors: Koh Jing Jie Augustine , Hussein Maytham , Rao Gauri G. , Li Jian , Velkov Tony
Published: November 2022

Multidrug-resistant (MDR) Gram-negative pathogens remain an unmet public health threat. In recent times, increased rates of resistance have been reported not only to commonly used antibiotics, but also to the last-resort antibiotics, such as polymyxins. More worryingly, despite the current trends in resistance, there is a lack of new antibiotics in the drug-discovery pipeline. Hence, it is imperative that new strategies are developed to preserve the clinical efficacy of the current antibiotics, particularly the last-line agents. Combining conventional antibiotics such as polymyxins with non-antibiotics (or adjuvants), has emerged as a novel and effective strategy against otherwise untreatable MDR pathogens. This review explores the available literature detailing the latest polymyxin/non-antibiotic combinations, their mechanisms of action, and potential avenues to advance their clinical application.
The Bio-Diversity and the Role of Gut Microbiota in Postmenopausal Women with Luminal Breast Cancer Treated with Aromatase Inhibitors: An Observational Cohort Study
Authors: Lasagna Angioletta , De Amici Mara , Rossi Chiara , Zuccaro Valentina , Corbella Marta , Petazzoni Greta , Comandatore Francesco , Sacchi Lucia , Testa Giorgia , Ferraris Elisa , Rizzo Gianpiero , Tancredi Richard , Ferrari Alessandra , Lucioni Marco , Sacchi Paolo , Bruno Raffaele , Pedrazzoli Paolo
Published: November 2022

The interactions between aromatase inhibitors (AI) in breast cancer (BC) and gut microbiota (GM) have not been completely established yet. The aim of the study is to evaluate the bio-diversity of GM and the relationship between GM, inflammation and tumor-infiltrating lymphocytes (TILs) in postmenopausal women with BC during adjuvant AI treatment compared to women with disease relapse during or after one year of AI therapy (“endocrine-resistant”). We conducted a monocenter observational case-control study. Eighty-four women with BC (8 cases, 76 controls) were enrolled from 2019 to 2021. We observed a significant difference in the mean microbial abundance between the two groups for the taxonomic rank of order (p 0.035) and family (p 0.029); specifically, the case group showed higher diversity than the control group. Veillonella reached its maximum abundance in cases (p 0.022). Cytokine levels were compared among the groups created considering the TILs levels. We obtained a statistically significant difference (p 0.045) in IL-17 levels among the groups, with patients with low TILs levels showing a higher median value for IL-17 (0.15 vs. 0.08 pg/mL). Further studies about the bio-diversity in women with BC may lead to the development of new biomarkers and targeted interventions.
Phenotype of Coxiella burnetii Strains of Different Sources and Genotypes in Bovine Mammary Gland Epithelial Cells
Authors: Sobotta Katharina , Bonkowski Katharina , Heydel Carsten , Henning Klaus , Menge Christian
Published: November 2022

Despite the high prevalence of C. burnetii in dairy herds and continuous shedding via milk by chronically infected cows, bovine milk is not recognized as a relevant source of human Q fever. We hypothesized that the bovine mammary gland epithelial cell line PS represents a suitable in vitro model for the identification of C. burnetii-strain-specific virulence properties that may account for this discrepancy. Fifteen C. burnetii strains were selected to represent different host species and multiple loci variable number of tandem repeat analysis (MLVA) genotypes (I, II, III and IV). The replication efficiencies of all strains were similar, even though strains of the MLVA-genotype II replicated significantly better than genotype I strains, and bovine and ovine isolates replicated better than caprine ones. Bovine milk isolates replicated with similar efficiencies to isolates from other bovine organs. One sheep isolate (Cb30/14, MLVA type I, isolated from fetal membranes) induced a remarkable up-regulation of IL-1β and TNF-α, whereas prototypic strains and bovine milk isolates tended to suppress pro-inflammatory responses. While infection with strain Nine Mile I rendered the cells partially refractory to re-stimulation with E. coli lipopolysaccharide, Cb30/14 exerted a selective suppressive effect which was restricted to IL-6 and TNF-α and spared IL-1β. PS cells support the replication of different strains of C. burnetii and respond in a strain-specific manner, but isolates from bovine milk did not display a common pattern, which distinguishes them from strains identified as a public health concern.
Genome-Wide Identification and Characterisation of Stress-Associated Protein Gene Family to Biotic and Abiotic Stresses of Grapevine
Authors: Sun Xiaoye , Xia Xue , Guan Xin
Published: November 2022

Grapevine is one of the earliest domesticated fruit crops and prized for its table fruits and wine worldwide. However, the concurrence of a number of biotic/abiotic stresses affects their yield. Stress-associated proteins (SAPs) play important roles in response to both biotic and abiotic stresses in plants. Despite the growing number of studies on the genomic organisation of SAP gene family in various species, little is known about this family in grapevines (Vitis vinifera L.). In this study, a total of 15 genes encoding proteins possessing A20/AN1 zinc-finger were identified based on the analysis of several genomic and proteomic grapevine databases. According to their structural and phylogenetics features, the identified SAPs were classified into three main groups. Results from sequence alignments, phylogenetics, genomics structure and conserved domains indicated that grapevine SAPs are highly and structurally conserved. In order to shed light on their regulatory roles in growth and development, as well as the responses to biotic/abiotic stresses in grapevine, the expression profiles of SAPs were examined in publicly available microarray data. Bioinformatics analysis revealed distinct temporal and spatial expression patterns of SAPs in various tissues, organs and developmental stages, as well as in response to biotic/abiotic stresses. This study provides insight into the evolution of SAP genes in grapevine and may aid in efforts for further functional identification of A20/AN1-type proteins in the signalling cross-talking induced by biotic/abiotic stresses.
HepCare Plus: Enhancing Primary Care Identification and Treatment of Hepatitis C Virus in High-Risk Individuals
Authors: O’Gorman Tessa , Lambert John S. , McHugh Tina , Cullen Walter , Avramovic Gordana , Federico Raffaele , West Bernard , O’Kelly Brendan , Vidal Louise , Farrell Jeremy , Broughan John , O’Connor Eileen , Woo James
Published: November 2022

Hepatitis C Virus (HCV) disproportionately affects people who inject drugs, migrants, prisoners and the homeless. An integrated, peer-led model of care involving primary and secondary care is required to enhance the identification and treatment of HCV in these marginalised groups. HepCare Plus builds on the network and achievements of HepCare Europe (a co-funded Third Health Programme of the European Union/Health Service Executive project). It further identifies those not accessing care and facilitates prompt assessment and treatment of those diagnosed with HCV, with the aid of a peer support worker (PSW) and a community HCV nurse specialist. Of 109 individuals identified and assessed for HCV treatment, 100 commenced HCV treatment. Despite interruptions to treatment (COVID-19 pandemic and national health service cyberattack) there was a high-level of treatment completion with PSW engagement (98%, n = 98). Eighty (73%) individuals were previously aware of a positive HCV status, highlighting the ongoing need to address barriers preventing marginalised groups from engaging with care. HepCare Plus reiterates the defining role of peer-led community interventions in HCV treatment engagement and the need for continuous open-ended HCV care. It provides a sustainable framework to meaningfully combat HCV and achieve the United Nations Sustainable Development Goal of HCV elimination by 2030.
Comparative Genomics Reveal the Utilization Ability of Variable Carbohydrates as Key Genetic Features of Listeria Pathogens in Their Pathogenic Lifestyles
Authors: Lu Qunfeng , Zhu Xiaoying , Long Qinqin , Yi Xueli , Yang Anni , Long Xidai , Cao Demin
Published: November 2022

Background: L. monocytogenes and L. ivanovii, the only two pathogens of Listeria, can survive in various environments, having different pathogenic characteristics. However, the genetic basis of their excellent adaptability and differences in pathogenicity has still not been completely elucidated. Methods: We performed a comparative genomic analysis based on 275 L. monocytogenes, 10 L. ivanovii, and 22 non-pathogenic Listeria strains. Results: Core/pan-genome analysis revealed that 975 gene families were conserved in all the studied strains. Additionally, 204, 242, and 756 gene families existed uniquely in L. monocytogenes, L. ivanovii, and both, respectively. Functional annotation partially verified that these unique gene families were closely related to their adaptability and pathogenicity. Moreover, the protein–protein interaction (PPI) network analysis of these unique gene sets showed that plenty of carbohydrate transport systems and energy metabolism enzymes were clustered in the networks. Interestingly, ethanolamine-metabolic-process-related proteins were significantly enriched in the PPI network of the unique genes of the Listeria pathogens, which can be understood as a determining factor of their pathogenicity. Conclusions: The utilization capacity of multiple carbon sources of Listeria pathogens, especially ethanolamine, is the key genetic basis for their ability to adapt to various environments and pathogenic lifestyles.
Anisakis Infection in the Spotted Flounder Citharus linguatula (Pleuronectiformes: Citharidae) Caught in the Gulf of Cadiz (Area FAO 27-ICES IXa) Appears to Negatively Affect Fish Growth
Authors: Morales-Yuste Manuel , Sánchez-Yebra Waldo , Garrido Mario , Benítez Rocío , Adroher Francisco Javier
Published: November 2022

Spotted flounder (Citharus linguatula L.) caught in the Gulf of Cadiz (area FAO 27 ICES IXa) were examined for Anisakis larvae and to assess the possible risk of anisakiasis in humans through consumption of this fish. Larvae of the genera Anisakis and Hysterothylacium were identified in the analysis of 128 purchased fish specimens. All Anisakis larvae corresponded to type I. Molecular analysis showed the presence of A. pegreffii, A. simplex s.s., and recombinant genotype between the two. The prevalence of Anisakis was 9.4% with a mean intensity of 1.42, while for Hysterothylacium the values were 12.5% and 1.06. The length and weight of the fish, but not Fulton’s condition factor, varied significantly between infected and uninfected fish. The prevalence of Anisakis increased with fish length, with no fish parasitized with Anisakis measuring less than 15.5 cm (2–2.5 years old), which is probably related to the reported dietary change of these fish at around 2 years of age. Fish not parasitized with any of these nematodes showed positive allometric growth, while those parasitized only with Anisakis showed negative allometric growth. When comparing both groups including only fish ≥ 15.5 cm (the smallest size of Anisakis-infected fish), the difference is shown to be statistically significant (p = 0.01), suggesting that Anisakis infection of spotted flounder negatively affects fish growth even when parasite intensity is low, which may have important economic repercussions. Finally, the low prevalence and, above all, intensity of Anisakis in these fish, as well as the habit of consuming this fish fried in oil in our geographical area, means that the risk of acquiring anisakiasis through consumption of this fish is low.
Spectrum and Density of Gamma and X-ray Induced Mutations in a Non-Model Rice Cultivar
Authors: Jankowicz-Cieslak Joanna , Hofinger Bernhard J. , Jarc Luka , Junttila Sini , Galik Bence , Gyenesei Attila , Ingelbrecht Ivan L. , Till Bradley J.
Published: November 2022

Physical mutagens are a powerful tool used for genetic research and breeding for over eight decades. Yet, when compared to chemical mutagens, data sets on the effect of different mutagens and dosages on the spectrum and density of induced mutations remain lacking. To address this, we investigated the landscape of mutations induced by gamma and X-ray radiation in the most widely cultivated crop species: rice. A mutant population of a tropical upland rice, Oryza sativa L., was generated and propagated via self-fertilization for seven generations. Five dosages ranging from 75 Gy to 600 Gy in both X-ray and gamma-irradiated material were applied. In the process of a forward genetic screens, 11 unique rice mutant lines showing phenotypic variation were selected for mutation analysis via whole-genome sequencing. Thousands of candidate mutations were recovered in each mutant with single base substitutions being the most common, followed by small indels and structural variants. Higher dosages resulted in a higher accumulation of mutations in gamma-irradiated material, but not in X-ray-treated plants. The in vivo role of all annotated rice genes is yet to be directly investigated. The ability to induce a high density of single nucleotide and structural variants through mutagenesis will likely remain an important approach for functional genomics and breeding.
Identification and Comparison of Bioactive Components of Two Dryopteris sp. Extract Using LC-QTOF-MS
Authors: Kim Yangseon , Lim Da Jung , Song Jeong-Sup , Kim Jung-Ae , Lee Byoung-Hee , Son Youn Kyoung
Published: November 2022

Dryopteris sp. is known for its various pharmacological effects and is used as a traditional medicine in Asia. The present study investigated the chemical composition and antimicrobial activity of Dryopteris sp. distributed in Korea. The chemical compounds in the ethanolic extracts of Dryopteris lacera and Dryopteris bissetiana were investigated by ultra-high performance liquid chromatography–quadrupole time-of-flight–mass spectrometry analysis and identified by exploring the UNIFI traditional medicine library. Flavonoids such as juglanin, 6-hydroxyluteolin 7-O-laminaribioside, peltatoside, kaempferitrin, hyperoside, and astragalin were identified in both D. lacera and D. bissetiana. Neochlorogenic acid was identified as a caffeoylquinic acid in D. bissetiana. Both extracts of D. lacera and D. bissetiana exhibited antibacterial activity against Gram-positive pathogens, Staphylococcus aureus and Streptococcus mutans. The minimum inhibitory concentration of D. bissetiana against S. aureus was less than 625 ppm. The antibacterial activity was attributed to the identified phenolic compounds, juglanin, 6-hydroxyluteolin 7-O-laminaribioside, kaempferitrin, astragalin, and neochlorogenic acid. Therefore, D. lacera and D. bissetiana can be used as Gram-positive selective antibiotics for further investigation.
Effect of Pyrolysis Temperature on the Sorption of Cd(II) and Se(IV) by Rice Husk Biochar
Authors: Li Zheyong , Su Qu , Xiang Luojing , Yuan Yajun , Tu Shuxin
Published: November 2022

This study investigated the removal of metal cations (Cd(II)) and metalloid anions (Se(IV)) from their aqueous solution by using agricultural waste (rice husk biochar). Rice husk biochar samples were prepared under 300, 500, and 700 °C pyrolysis conditions and their physicochemical properties were characterized. Aqueous Cd(II) and Se(IV) sorption kinetics and isotherms of rice husk biochar were studied. The results showed that the yield of rice husk biochar decreased from 41.6% to 33.3%, the pH increased from 7.5 to 9.9, and the surface area increased from 64.8 m2/g to 330.0 m2/g as the pyrolysis temperature increased from 300 °C to 700 °C. Under the experimental conditions, at increasing preparation temperatures of rice husk biochar, the sorption performance of Cd(II) and Se(IV) was enhanced. The sorption capability and sorption rate were considerably higher and faster for Cd(II) ions than for Se(IV) ions. Cd(II) sorption was found to reach equilibrium faster, within 150 min, while Se(IV) sorption was slower and reached equilibrium within 750 min. The maximum sorption capacities of cadmium and selenium by rice husk biochar were 67.7 mg/g and 0.024 mg/g, respectively, according to Langmuir model fitting.
Allelopathy and Allelochemicals of Solidago canadensis L. and S. altissima L. for Their Naturalization
Authors: Kato-Noguchi Hisashi , Kato Midori
Published: November 2022

Solidago canadensis L. and Solidago altissima L. are native to North America and have naturalized many other continents including Europa and Asia. Their species is an aggressive colonizer and forms thick monospecific stands. The evidence of the allelopathy for S. canadensis and S. altissima has accumulated in the literature since the late 20th century. The root exudates, extracts, essential oil and rhizosphere soil of S. canadensis suppressed the germination, growth and the arbuscular mycorrhizal colonization of several plants, including native plant species. Allelochemicals such as fatty acids, terpenes, flavonoids, polyphenols and their related compounds were identified in the extracts and essential oil of S. canadensis. The concentrations of total phenolics, total flavonoids and total saponins in the rhizosphere soil of S. canadensis obtained from the invasive ranges were greater than those from the native ranges. Allelochemicals such as terpenes, flavonoids, polyacetylene and phenols were also identified in the extracts, essential oil and the rhizosphere soil in S. altissima. Among the identified allelochemicals of S. altissima, the cis-dehydromatricaria ester may be involved in the allelopathy considering its growth inhibitory activity and its concentration in the rhizosphere soil. Therefore, the allelopathy of S. canadensis and S. altissima may support their invasiveness, naturalization and formation of thick monospecific stands. This is the first review article focusing on the allelopathy of both of S. canadensis and S. altissima.
An Inventory of South African Medicinal Plants Used in the Management of Sexually Transmitted and Related Opportunistic Infections: An Appraisal and Some Scientific Evidence (1990–2020)
Authors: Mongalo Nkoana I. , Raletsena Maropeng V.
Published: November 2022

The current work is aimed at generating the first inventory of South African medicinal plants used in the treatment of sexually transmitted and related opportunistic infections associated with HIV-AIDS. This is important in assisting researchers to access a list of plant species to evaluate for potential phytocompounds, as this area of research is greatly lagging in South Africa. A total of 335 medicinal plants from 103 families have been documented in the current work. The most represented families are Fabaceae (11.64%) and Asteraceae (6.27%). Herbs constitute 36.53%, trees 32.34%, shrubs 29.04%, climbers 1.80% and parasites 0.30%. It is worrying that on the plant parts used, the roots constitute 47.18%, while leaves and stem bark yield 16.62 and 15.01%, respectively. Catharanthus roseus exhibited the highest number of citations (19), while Peltophorum africanum had 14 and both Carica papaya and Vachelia karoo had 12. In the mode of administration of the reported medicinal plant species, most of the plants are boiled and taken orally (48.22%), while other plant species are used as mouth washes (3.25%). Although there is reasonable in vitro activity of some of the plant species, validating the relevance of use, there is still a need to explore the mode of action of such plant species; isolated compounds and possible derivatives thereof are of paramount importance and need to be explored as well. Furthermore, toxicological aspects of such plant species need to be explored.
Genomic Characteristics of Elite Maize Inbred Line 18-599 and Its Transcriptional Response to Drought and Low-Temperature Stresses
Authors: Cao Yang , Qu Jingtao , Yu Haoqiang , Yang Qingqing , Li Wanchen , Fu Fengling
Published: November 2022

Elite inbred line 18-599 was developed via triple test cross from introduced hybrid P78599 and used as parents of dozens of maize hybrids adapting to the diverse ecological conditions of the maize ecological region in Southwest China. In this study, its genomic DNA was resequenced and aligned with the B73 genome sequence to identify single nucleotide polymorphism (SNP), and insertion (In) and deletion (Del) loci. These loci were aligned with those between B73 and 1020 inbred lines in the HapMap database to identify specific variation loci of 18-599. The results showed that there were 930,439 specific SNPs and 358,750 InDels between 18-599 and the 1020 lines. In total, 21,961 of them showed significant impacts on the functions of 12,297 genes, such as frameshift, change of splicing site, stop gain, change of start site, and stop loss. Phylogenetic analysis showed that 18-599 was closely related to inbred lines ZEAxujRAUDIAAPE and 2005-4, but far from some inbred lines directly isolated from P78599. This result indicated that 18-599 not only pyramided the elite genes of P78599, but also acquired genetic divergence during the repetitive backcrosses of triple test cross to confer its elite agronomic characteristics. Subsequently, the RNA of 18-599 was sequenced. The aligned 9713 and 37,528 of the 165,098 unigenes were screened and aligned with annotated transcripts of the B73 genome differentially expressed under drought and low-temperature stress, respectively, and their functions were involved in the responses to these stresses. The quantitative PCR results of fourteen random genes verified the RNA sequencing results. These findings suggest that the transcriptional responses of many resistance-related genes were an important mechanism for 18-599 to adapt to diverse ecological conditions.
Fine Mapping of Stripe-Rust-Resistance Gene YrJ22 in Common Wheat by BSR-Seq and MutMap-Based Sequencing
Authors: Chen Can , Hao Weihao , Wu Jingchun , Si Hongqi , Xia Xianchun , Ma Chuanxi
Published: November 2022

Identification and accurate mapping of new resistance genes are essential for gene pyramiding in wheat breeding. The YrJ22 gene is a dominant stripe-rust-resistance gene located at the distal end of chromosome 2AL, which was identified in a leading Chinese-wheat variety, Jimai 22, showing high resistance to CYR32, a prevalent race of Puccinia striiformis tritici (Pst) in China. In the current study, 15 F1 and 2273 F2 plants derived from the cross of Jimai 22/Avocet S were used for the fine-mapping of YrJ22. The RNA-Seq of resistant and susceptible bulks of F2 plants (designated BSR-Seq) identified 10 single-nucleotide polymorphisms (SNP) in a 12.09 Mb physical interval on chromosome 2AL. A total of 1022 EMS-induced M3 lines of Jimai 22 were screened, to identify susceptible mutants for MutMap analysis. Four CAPS markers were developed from SNPs identified using BSR-Seq and MutMap. A linkage map for YrJ22 was constructed with 11 CAPS/STS and three SSR markers. YrJ22 was located at a 0.9 cM genetic interval flanked by markers H736 and H400, corresponding to a 340.46 kb physical region (768.7–769.0 Mb), including 13 high-confidence genes based on the Chinese Spring reference genome. TraesCS2A01G573200 is a potential candidate-gene, according to linkage and quantitative real-time PCR (qPCR) analyses. The CAPS marker H732 designed from an SNP in TraesCS2A01G573200 co-segregated with YrJ22. These results provide a useful stripe-rust-resistance gene and molecular markers for marker-assisted selection in wheat breeding and for further cloning of the gene.