Wastewater surveillance forSalmonellaTyphi and its association with seroincidence of enteric fever in Vellore, India
Authors: Abraham Dilip , Kathiresan Lalithambigai , Sasikumar Midhun , Aiemjoy Kristen , Charles Richelle C. , Kumar Dilesh , Srinivasan Rajan , Troman Catherine , Gray Elizabeth , Uzzell Christopher B. , John Jacob , Veeraraghavan Balaji , Grassly Nicholas C. , Mohan Venkata Raghava 
Published: July 2024

Background

Blood culture-based surveillance for typhoid fever has limited sensitivity, encountering operational challenges in resource-limited settings. Environmental surveillance targetingSalmonellaTyphi (S. Typhi) shed in wastewater (WW), coupled with cross-sectional serosurveys ofS.Typhi-specific antibodies estimating exposure to infection, emerges as a promising alternative.

Methods

We assessed the feasibility and effectiveness of wastewater (WW) and sero-surveillance for S. Typhi in Vellore, India, from May 2022 to April 2023. Monthly samples were collected from 40 sites in open drainage channels and processed using standardized protocols. DNA was extracted and analyzed via quantitative PCR for S. Typhi genes (ttr, tviB, staG) and the fecal biomarker HF183. Clinical cases of enteric fever were recorded from four major hospitals, and a cross-sectional serosurvey measured hemolysin E (HlyE) IgG levels in children under 15 years to estimate seroincidence.

Results

7.50% (39/520) of grab and 15.28% (79/517) Moore swabs were positive for all 3S .Typhi genes. Moore swab positivity was significantly associated with HF183 (adjusted odds ratio (aOR): 3.08, 95% CI: 1.59 – 5.95) and upstream catchment population (aOR: 4.67, 1.97 – 11.04), and there was increased detection during monsoon season - membrane filtration (aOR: 2.99, 1.06 – 8.49), and Moore swab samples (aOR: 1.29, 0.60 – 2.79). Only 11 blood culture-confirmed typhoid cases were documented over the study period. Estimated seroincidence was 10.4/100 person-years (py) (95% CI: 9.61 - 11.5/100 py). The number ofS.Typhi positive samples at a site was associated with the estimated sero- incidence in the site catchment population (incidence rate ratios: 1.14 (1.07-1.23) and 1.10 (1.02-1.20) for grab and Moore swabs respectively.

Conclusions

These findings underscore the utility and effectiveness of alternate surveillance approaches to estimating the incidence of S. Typhi infection in resource-limited settings, offering valuable insights for public health interventions and disease monitoring strategies where conventional methods are challenging to implement.

Author Summary

Our study explores the potential of detectingSalmonellaTyphi in wastewater, informing public health strategies, guiding vaccination campaigns, and monitoring the effectiveness of interventions, contributing to better disease control and prevention policies.

S. Typhi positivity rates of 7.50% and 15.30% were observed in grab samples and Moore swabs, respectively, and significant correlations were found betweenS. Typhi positivity in Moore swabs and fecal contamination marker levels in wastewater samples.

The study also estimated that the community seroincidence for typhoid 10.40/100 person-years andS. Typhi detections in WW positively correlated with seroincidence.

The study reveals significant associations between wastewater S. Typhi positivity and typhoid seroincidence, seasonal variations, and population dynamics, providing deeper insights into the epidemiology of typhoid fever.