bluuebunny/crossref_metadata_2025 huggingface dataset:
Web-Based New Student Admission Information System Using Waterfall Method
Authors: Aldi Febri
Published: January 2022

The current development of information technology on the activities of people's daily lives is very influential. Where in doing an activity that takes a lot of time can be shortened to complete it. One of the things that we can see at this time is in the field of education. Education service technology is feasible if the government, education service providers, and the community all work together. The problem is how to build information system technology that can provide educational service processes without intervening in existing operational standards. The New Student Admissions Information System is a website-based platform designed to enable public access to information about schools and the new student registration process. The waterfall approach was used to construct this information system, together with the PHP programming language, the Codeigniter 3 framework, the MVC pattern, and the MySQL database. The results obtained in the form of a new student admission information system website that provides services related to registration, and processing of registration data by the school administration. With this research, it is expected to facilitate new student admission officers at Al Azhar Islamic Elementary School 32 Padang to process data well, and help parents to register their children as new students at Al Azhar Islamic Elementary School 32 Padang.
Comparison Decision Tree and Logistic Regression Machine Learning Classification Algorithms to determine Covid-19
Authors: Arista Artika
Published: January 2022

Many people today are unsure whether they have COVID-19. The frequent fever, dry cough, and sore throat are all signs and symptoms of COVID-19. If a person has signs or symptoms of coronavirus disease 2019 (COVID-19), he/she should see the doctor or go to a clinic as soon as possible. As a result, it's vital to learn and comprehend the fundamental differences. COVID-19 can cause a wide range of symptoms. The experiments were carried out using two Machine Learning Classification Algorithms, namely Decision Tree (DT) and Logistic Regression (LR). Both algorithms were written and analyzed using the Python program in Jupyter Notebook 6.4.5. From the results obtained in the experiments of covid symptoms dataset, on average, the DT model has obtained the best cross-validation average and the testing performance average compared to the LR machine learning models. For cross-validation results, the DT model has achieved an accuracy of 98.0%. For performance testing, the DT model has achieved an accuracy of 98.0%. The LR has obtained the second-best result on the average of cross-validation performance and the testing results. For cross-validation results, the LR model has achieved an accuracy of 96.0%. For performance testing, the LR model has achieved an accuracy of 97.0%. Consequently, the DT for the COVID-19 symptoms dataset is outperforming the LR for cross-validation and testing results.
Plantas medicinais no tratamento de ansiedade e depressão: Uma revisão
Authors: Nobrega José Cândido da Silva , Batista Alan Vinícius de Araújo , Silva Osvaldo Soares da , Belchior Verônica Cristian Soares de , Lacerda Wendell de Almeida , Belchior Sandra Maijane Soares de
Published: January 2022

Há uma grande diversidade de plantas no Brasil, e muitas delas, ao serem estudadas, apresentam elevado teor nutricional, presença de antioxidante, entre outras propriedades, podendo ter um potencial farmacológico significativo e consequentemente gerando aplicações em diversas áreas como saúde, cosmética e culinária. As plantas medicinais são usadas por grande parte da população mundial, como um recurso medicinal alternativo para o tratamento de diversas doenças, visto que em muitas comunidades, representam um recurso mais acessível em diversos sentidos em relação aos medicamentos alopáticos. Destaca-se a utilização das plantas medicinais para fins terapêuticos, em que muitas atividades biológicas conferidas às plantas foram demonstradas, dentre elas a ação no sistema nervoso central, sendo utilizadas para o tratamento e/ou prevenção de transtornos psicossociais como ansiedade e depressão. Sendo assim, o presente estudo tem como objetivo realizar uma revisão bibliográfica sobre as plantas medicinais mais utilizadas no tratamento da ansiedade e depressão. Verificou-se que nos tratamentos, as plantas medicinais e os fitoterápicos são vistos como uma alternativa viável para o tratamento dos quadros de ansiedade e depressão, visto que possuem menos efeitos colaterais. Sendo assim, a presente pesquisa revisou as espécies: Hypericum perforatum L.; Passiflora incarnata L.; Melissa Officinalis L.; Chamomilla recutita L.; Piper methysticum G.; Erythrina verna; Humulus lupulus L. e Crataegus oxyacantha L. Foi possível apresentar a efetividade de todas as espécies citadas, com as descrições encontradas em estudos da literatura.
A importância da perícia técnica para a construção civil
Authors: Lima Thaygno Azevedo , Dourado Graziela Rios de Araújo , Silva Wellington César Teles da
Published: January 2022

A construção é um participante importante nas economias dos países em desenvolvimento. A engenharia tem procurado agregar novas tecnologias, principalmente no campo da globalização, o que aumenta a competição. Além da necessidade de inovação, o engenheiro tem dificuldade em encontrar o ajuste certo para o projeto, custo, qualidade e prazo, e a meta de sucesso de gerenciamento nesses novos cenários. Nesse sentido, este estudo teve por objetivo relatar sobre a importância do laudo pericial para a construção civil. Trata-se de uma pesquisa de revisão da literatura, de metodologia descritiva, apresentada de modo qualitativo. A coleta de dados foi realizada mediante busca eletrônica, nas bases de dados Scientific Electronic Library Online (SCIELO) e Google Scholar. A temática em questão, foi desenvolvida tendo o enfoque de pesquisa voltado para o objetivo do estudo. A partir do crescente desenvolvimento com que o setor da construção civil tem se deparado, bem como o surgimento de novas técnicas e tecnologias, pode-se garantir que, por um lado, esta área está reunindo condições para realizar cada vez mais trabalhos de alta qualidade. Em contrapartida, o campo da construção sofre grandes apreensões, desde a falta de qualidade dos projetos a serem executados até a de mão de obra qualificada para a execução das edificações, sendo esta, certamente, o mais grave dos problemas.
Proposta de intervenção psicopedagógica em caso de dislexia a partir das perspectivas do filme “Como Estrelas na Terra”
Authors: Pinto Vinícius Rodrigues Arruda , Gomes Jamila Reis , Fonseca Felipe Vidigal Sette da , Fernandes Marcela de Melo
Published: January 2022

A Arte terapia é um método que tem como objetivo proporcionar a saúde mental e sua prática incorpora diferentes linguagens artísticas. No presente artigo, objetivou-se fazer um comparativo entre a experiência do protagonista do filme Como Estrelas na Terra, o portador de dislexia Ishaan, e a também portadora de dislexia Clarice, para entender a importância da arteterapia como aliada na intervenção psicoterapêutica. Objetivamos ainda entender como a institucionalização do sistema educacional atual interfere na educação inclusiva, contribuindo para o desenvolvimento de neurose noogênica em estudantes com dislexia. Nesse contexto, o papel que a família enquanto instituição também é preponderante para promover sensibilização e valorização das formas singulares no processo de ensino-aprendizagem de portadores de dislexia. A partir da proposição da Terapia de Intenção Paradoxal associada aos recursos arte terapêuticos propusemos uma intervenção no caso Clarice, tendo a arte como aliada no alvorecer do sentido da vida. do presente trabalho é potencializar maneiras singulares do processo de livre criação-aprendizagem, sobretudo a partir do desfecho de Ishaan, propiciando mudanças nas esferas afetivas, interpessoais e relacionais de estudantes com dislexia.
Utility of vitamin D serum evaluation and supplementation in dogs and cats’ medical routine: a review
Authors: Cruz-Filho João da , Rocha Leandro Branco , Santana Alessandra de , Lobão Gabriel de Araújo
Published: January 2022

Vitamin D is traditionally known for its role in regulating calcium homeostasis and consequently maintaining bone integrity and health. However, more recently, it has been observed that it has a series of previously unknown non-canonical functions, such as maintenance of immunity and intestinal mucosa integrity. Furthermore, it has been shown to be associated with the progression or perpetuation of several diseases since its serum concentration is reduced during the course of the conditions. Therefore, we sought to investigate, through a literature review, the usefulness of serum vitamin D measurement in dogs and cats as a prognostic factor for different diseases, as well as the possible therapeutic effect of supplementing this vitamin in the correction of those illnesses. Vitamin D is in fact deficient in gastroenteric diseases, such as protein-losing enteropathy, infectious diseases, such as canine leishmaniasis, heart and kidney disease, among others. However, it is necessary to understand more properly about the physiological role of vitamin D in health, so that we can also understand it in disease. There are also too few data reports supporting supplementing this vitamin as main or adjuvant therapy in the treatment of any disease, but evidence points to the usefulness of vitamin D serum measurement as a prognostic predictor for dogs and cats.
Parada Cardiorrespiratória em Unidade de Internação Cirúrgica: tecnologia educativa para sistematizar ações de enfermagem
Authors: Coelho Camilada Silva Vale , Lima Fernando Conceição de , Nazaré Nancy de Souza Felipe , Santana Victoria Karolina Santos , Oliveira Rosivalda Ferreira de , Franco Alex Miranda , Simor Alzinei , Mendes Clarissa Porfírio , Dias Gabriela de Nazaré e Silva , Guimarães Danielle Casseb , Moia Gracilene Wanzeler , Souto Maria Madalena Castro , Ramalho Matheus Almeida , Silva Thalita Mariana Gonçalves da , Freitas Luana da Silva
Published: January 2022

Objetivo: produzir uma tecnologia assistencial para a equipe de enfermagem para detecção de PCR e início precoce de manobras de RCP. Metodologia: Trata-se de um estudo metodológico, desenvolvido em três etapas: Etapa 1 – Definição do escopo, realizado em um hospital credenciado como Centro de Alta Complexidade em Oncologia. A pesquisa se desenvolveu com enfermeiros, enfermeiros residentes e técnicos de enfermagem, Etapa 2 – Levantamento bibliográfico - demonstrou uma escassez de produções acerca do tema abordado, o que justifica a criação do instrumento descrito neste estudo e Etapa 3 – Desenvolvimento - Construiu-se o fluxograma.  Resultado: Obteve-se respostas permitindo a comparação antes e depois da aplicação de um instrumento de coleta criado pelos próprios autores e que propiciou a criação da tecnologia. Conclusão: A criação do fluxograma como tecnologia assistencial para sistematizar ações de enfermagem na clínica, possibilitou oferecer de forma clara e concisa a assistência no local, com ações de enfermagem para aumentar a sobrevida de pacientes vítimas de PCR.
Impacts and effects of the use of medications by crew and passengers during air transport
Authors: Soares Júnior Gilberto Gomes , Ayub Omar , Gasparim Jose Carlos , Sampaio Luciana Maria Malosá , Contador José Celso , Barbosa Antônio Pires , Monken Sonia Francisca de Paula , Martens Mauro Luiz , Martens Cristina Dai Prá , Satyro Walter Cardoso
Published: January 2022

During a flight, changes occur in the human body, whose possible reactions can influence the well-being and quality of health of medicine users, subject of scarce studies. The aim of this paper is to analyze the impacts and effects of medication use during air transport. Through a literature review and documentary research methodology, relevant facts about the precautions that should be taken regarding the use of medication in flights were considered. As a practical contribution, some important medications and their possible effects during flights are presented, as well as some warnings about medication interactions, and some advice for better quality air travel for the health of passengers and crew are given. As a theoretical contribution, this study gathers the scattered information presented in the literature about the problems that the use of medications during a flight can cause to the human body and possible recommendations. As social contribution, this study warns about the risks of self-medication, and establishes a basis for future discussions with society about the use of medication in flights.
PENGARUH TUNJANGAN KINERJA (TUKIN) DAN MOTIVASI TERHADAP KINERJA PEGAWAI POLITEKNIK KELAUTAN DAN PERIKANAN SORONG
Authors: Saharuddin Saharuddin , Watunglawar B. , Tafalas Muhammad Guzali
Published: January 2022

The Purpose of this research is to process, analyze, prove empirically and discuss: (a) the effect of perfrormance benefits on the performance of Sorong Maritime and Fisheries Polythecnic employess; (b) The influence of motivation on the performance of Sorong Polytechnic Maritime and Fisheries employess; and (c) The join effect of performance and motivation allowance on the performance of Sorong Polytechnic Maritime and Fisheries employees. This research was conducted in September to November 2018. The author collected data through questionnaires and observation for a month. The population in this study is the Polytechnic Maritime and Fisheries totaling 66 people. This study is a study of facts in the form of opinions/opinion, attitudes, experiences or characteristics of people (respondents) as subject data in the form of primary data obtained from interviews, questionnaire and observation, also seconday data. Analysis of research data using descriptive statistics, with a view to finding strong relationship between variables through correlation analysis with regression analysis, by making comparisons by comparing the average sampel data or population. The results showed that (1) Performance allowance (X1) with all the components of its formation had no effect on employee performance (Y), so the first hypothesis stating that performance allowance (X1) affected employee performance (Y) was not proven; (2) Motivation (X2) with all the components of its formation affect employee performance (Y), so the first hypothesis stating that Motivation (X2) influences employee performance (Y) is proven.
Kedudukan Hak Keperdataan Anak Luar Kawin Perspektif Hukum Islam
Authors: Susanto Muhammad Hajir , Puspitasari Yonika , Marwa Muhammad Habibi Miftakhul
Published: January 2022

Secara konstitusional setiap warga negara mempunyai hak keperdataan yang harus dijamin dan dilindungi. Tidak semua anak lahir bernasib baik. Ada anak dilahirkan dari ikatan perkawinan yang sah disebut anak sah. Sementara, disebut anak luar kawin yaitu anak yang lahir tidak dalam ikatan perkawinan yang sah di mana laki-laki dan perempuan yang masih perjaka dan perawan. Adanya perbedaan hak yang diterima anak sah dengan anak luar kawin menarik untuk dikaji menggunakan berbagai perspektif, salah satu perspektif hukum Islam. Penelitian ini merupakan penelitian normatif dengan menggunakan sumber data sekunder yang diperoleh melalui studi pustaka. Data yang diperoleh dianalisis secara deskriptif menggunakan pendekatan konsep hukum Islam. Nasab anak dalam hukum Islam dibedakan antara anak yang dibuahi tidak dalam perkawinan sah namun dilahirkan dalam perkawinan yang sah, dengan anak yang dibuahi dan dilahirkan di luar perkawinan yang sah. Kedudukan anak luar kawin menurut hukum Islam hanya memiliki hubungan keperdataan dengan ibu dan keluarga ibu. Adapun hubungan anak luar kawin dengan bapaknya menurut hukum Islam, yakni tidak memilik hak keperdataan berupa tidak ada hubungan nasab, tidak saling mewarisi, dan tidak dapat menjadi wali nikah.
BENTUK PENYALAHGUNAAN WEWENANG PEJABAT PEMERINTAH YANG TIDAK DAPAT DIPIDANA
Authors: Alti Putra Moh Alfatah
Published: January 2022

Bentuk kesalahan dalam penggunaan wewenang (misuse of authority), sebenarnya merupakan perbuatan yang beda pengertian dengan penyalahgunaan wewenang (abuse of power) sehingga berkaitan dengan dapat tidaknya dipidana bentuk kesalahan tersebut. Penelitian yang dilakukan adalah penelitian dengan menggunakan metode penelitian kepustakaan (Library Research Methods). Bentuk Penyalahgunaan Wewenang yang tidak dapat dipidana merupakan penyalahgunaan wewenang dengan wujud kesalahan penggunaan wewenang (misuse of authority), sedangkan dalam Penyalahgunaan Wewenang yang dapat dipidana didalamnya terdapat bentuk kesalahan dengan corak kesengajaan sebagai maksud atau niat jahat (mens rea) untuk menyalahgunakan wewenang. Bentuk Penyalahgunaan Wewenang yang tidak dapat dipidana adalah penyalahgunaan wewenang dengan bentuk melampaui wewenang dan sewenang-wenang, karena dalam pengertiannya di Undang Undang Administrasi Pemerintahan tidak ada peralihan tujuan dari pemberian wewenang terkecuali merupakan kesalahan administrasi dengan wujud kesalahan penggunaan wewenang (misuse of authority). Sedangkan dalam Penyalahgunaan Wewenang yang dapat dipidana bentuk penyalahgunaan wewenangnya sesuai dengan pengertian dalam Undang Undang Administrasi Pemerintahan tentang mencampuradukkan wewenang yang dapat ditindaklanjuti dengan pemidanaan ketika peralihan tujuan wewenang itu disertai Niat Jahat (Mens Rea) dalam bentuk kesalahan dengan corak kesengajaan sebagai maksud untuk mencari keuntungan yang merugikan keuangan Negara atau perekonomian Negara.
Fiat Money Inconsistency and the Return of Dinar-Dirham as a Medium of Exchange in the Community
Authors: Burhanuddin Chairul Iksan , Hidayat M
Published: January 2022

Coronavirus ior ireferred ito ias icovid-19 ihas icaused ivarious ikinds iof inegative ieffects, iespecially iin ithe ieconomic ifield. iThe isoaring iunemployment irate, ithe icontraction iin ithe ivalue iof ithe irupiah iand iother icurrencies ihas iresulted iin ian iincreasingly isluggish ieconomic imovement iin irecent itimes iuntil ithe ipanic iof ithe ivalue iof igold icontinues ito irise iin irecent imonths. iThe iqualitative imethod iis iused iby iscientifically idescribing ithe iimpact iof ithe iCovid i19 ivirus icase ion ithe imovement iof icurrency iand igold ivalues. iThe iresults iof ithis istudy iindicate ithat ithe iCovid i19 ivirus ihas ia iquite inegative iimpact ion ithe ieconomic isector, iespecially ion icurrency iand igold ivalue imovements. iThe imovement iin ithe ivalue iof ithe irupiah icontracted ito itouch iRp.14,000 iwhile ithe ivalue iof igold itended ito irise isignificantly. iThe irising igold ivalue ishows ia ipositive itrend itowards ithe ipossible iimplementation iof ithe ilegal iuse iof ithe idinar iand idirham icurrency iin ithe icommunity. iGold iis ino ilonger iseen ias ia iSave iHaven, ibut iits irole ias ian iextension iof imuamalah iactivities i(buying iand iselling) iin ithe icommunity iis iincreasingly ivisible. iThere iis ipanic iand ipsychologically idisturbing ieconomic iand icommunity iactors iin iresponding ito ithis iincident.iCoronavirus ior ireferred ito ias icovid-19 ihas icaused ivarious ikinds iof inegative ieffects, iespecially iin ithe ieconomic ifield. iThe isoaring iunemployment irate, ithe icontraction iin ithe ivalue iof ithe irupiah iand iother icurrencies ihas iresulted iin ian iincreasingly isluggish ieconomic imovement iin irecent itimes iuntil ithe ipanic iof ithe ivalue iof igold icontinues ito irise iin irecent imonths. iThe iqualitative imethod iis iused iby iscientifically idescribing ithe iimpact iof ithe iCovid i19 ivirus icase ion ithe imovement iof icurrency iand igold ivalues. iThe iresults iof ithis istudy iindicate ithat ithe iCovid i19 ivirus ihas ia iquite inegative iimpact ion ithe ieconomic isector, iespecially ion icurrency iand igold ivalue imovements. iThe imovement iin ithe ivalue iof ithe irupiah icontracted ito itouch iRp.14,000 iwhile ithe ivalue iof igold
The Effect of Turnover Intention, Locus of Control, Audit Fee, Work Motivation on the Performance Quality of Medan City Public Accountant Firm
Authors: Basa Holy Maria , Yanti Lidia Yuni , Br. Pinem Delvi Meilani , Munthe Dora Kristyna , Lilia Wirda
Published: January 2022

The purpose of this study is to determine the effect of turnover intention, locus of control, audit fees, and work motivation on the quality of auditor performance at the Medan City Public Accounting Firm in 2021. The choice of the sector in this study is due to very tight competition conditions like now. In this case, the auditor must always maintain their performance so that audit quality is not compromised. Auditors must perform work by professional standards so that trust in the auditor can be maintained. The data used is sourced from quantitative data in the form of questionnaires. Using the Simple Random Sampling technique, a sample of 50 auditors was drawn from five Medan City KAPs.The results showed that locus of control, audit fees, and work motivation had a positive and significant effect on the quality of auditor performance at KAP Medan City. Meanwhile, the turnover intention has a negative effect and does not significantly affect the quality of auditor performance at KAP Medan City. But all variables are affected simultaneously.Â
Application of Quick Response Code Indonesian as a Payment Tool in Digitizing MSMEs
Authors: Anggarini Desy Tri
Published: January 2022

The purpose of the research is to contribute to the development of technology in the financial sector, to give influence to the community in terms of cashless, then also to provide insight into financial technology on financial literacy by using QRIS (Quick Response Code Indonesian). MSMEÂ  use facilitate their business activities by implementing fintech in terms of transaction management and the role of financial technology, is the QRIS (Quick Response Code Indonesian) application as a means of payment. The research method used in the research on the application of QRIS (Quick Response Code Indonesian) as a Payment Tool in digitizing MSMEs is a qualitative descriptive study using field research methods. This study uses interviews with 10 traders and 10 UMKM consumers who use QRIS. The research location is in BSD Modern Market, using non-probability techniques. Primary data taken directly from the object of research. With the direct observation method at the BSD modern market, South Tangerang with interview techniques for both consumers and traders who use QRIS as well as secondary data also obtained through various literatures. The result of the research is that QRIS as a digital payment tool for MSMEs is non-cash digital payment using Gopay, Ovo and others. QRIS has the potential to expand MSME sales, with QRIS an increase in digital sales traffic and a decrease in cash, minimizing the risk of paying counterfeit money, automatically recording transactions, building credit profiles for banks, making it easier to get working capital, payment of retribution, bills, purchasing goods in cash easily.
Sustainable management of populations impacted by harvesting and climate change
Authors: Stenseth NC , Ims RA , Sæther BE , Cadahía L , Herfindal I , Lee AM , Whittington JD , Yoccoz NG
Published: January 2022

The sustainable use of natural resources is critical for addressing the global challenges of today. Strategies for sustainable harvesting need to consider not only harvested species, but also other non-harvested species interacting with them in the same ecosystem. In addition, environmental variation needs to be considered, with climate change currently being one of the main sources of this variation. Understanding the consequences of complex interactions between different drivers and processes affecting dynamics of species and ecosystems across spatial scales requires large-scale integrative research projects. The Norwegian research initiative “Sustainable management of renewable resources in a changing environment: an integrated approach across ecosystems” (SUSTAIN) was launched to fill knowledge gaps related to the sustainable management of populations and ecosystems experiencing climate change. SUSTAIN investigated terrestrial, marine and freshwater ecosystems in boreal and Arctic regions, using both theoretical developments and empirical analyses of long-term data. This Climate Research Special contains both synthesis articles and original research exemplifying some of the approaches used in SUSTAIN. In this introduction we highlight 4 key topics addressed by SUSTAIN: (i) population structure, (ii) interactions between species, (iii) spatial processes, and (iv) adaptive management. These topics are fundamental to the understanding of harvested species from an ecosystem perspective, and to ecosystem-based management approaches, which we are striving to work towards.
THE RELATIONSHIP BETWEEN CORONAVIRUS FEAR AND ANXIETY IN PREGNANT WOMEN DURING COVID-19 OUTBREAK
Authors: DURMUŞ Mustafa , ŞENER Nurdilan , ERSÖĞÜTÇÜ Filiz
Published: January 2022

The aim of this research is to determine the relationship between coronavirus (COVID-19) fears and anxiety levels of pregnant women. The research was conducted in descriptive and cross-sectional design with 461 pregnant women between 15.10.2020-15.11.2020. Data were collected using personal information form, COVID-19 Fear Scale, and Beck Anxiety Scale. There was no statistically significant difference between the women's occupation, number of pregnancies, family structure, and income status, and the total mean scores of COVID-19 fear (p>0.05). While the coronavirus fear average score of pregnant women (21.29±6.08) was above the medium level, it was found to be at a moderate level for anxiety (15.85±9.80). It was determined that there was a significant positive difference between the COVID-19 fear and anxiety in pregnant women (r=.399, p<0.01). It can be recommended to provide telephone consultation service to pregnant women during the pandemic to reduce their fear and anxiety levels and inform them using mass communication methods.
Study on Employment Creation by Hotel Industries: A Case of Five Star Hotels of Kathmandu.
Authors: Jha Krishna Chandra , Rijal Nimananda
Published: January 2022

Abstract. Nepal Opened up to 1950external tourists since Sagarmatha was scaled up in 1953. ‘The first hotel of Kathmandu was Hotel Snow View, opened in early1950 to cater to the tourists. Most of the workers were hired from India. At present, There are 138-star hotels, 1151 Tourist Standard Hotel, and 43999 hotel beds in Nepal. The study found out that there are 16 five-star hotels in Kathmandu with an occupancy capacity of 1343 rooms 54 restaurants in an average of 3.37 in a hotel. Total employment in five-star hotels found 3065 among them 1966 male and 1099 female employees; the ratio between men to women is 1: 1.78. And the outcome is interpreted as 1 F= 1.78 M. The employee should have refreshing training in their respective field of work. The Government and HAN should join hands in retaining the well-trained employees. They should have retirement benefits as well.
Alteration of the Sitting and Standing Movement in Adult Spinal Deformity
Authors: Saad Eddy , Semaan Karl , Kawkabani Georges , Massaad Abir , Salibv Renee Maria , Mekhael Mario , Fakhoury Marc , Karam Krystel Abi , Jaber Elena , Ghanem Ismat , Lafage Virginie , Skalli Wafa , Rachkidi Rami , Assi Ayman
Published: January 2022

Adults with spinal deformity (ASD) are known to have spinal malalignment affecting their quality of life and daily life activities. While walking kinematics were shown to be altered in ASD, other functional activities are yet to be evaluated such as sitting and standing, which are essential for patients’ autonomy and quality of life perception. In this cross-sectional study, 93 ASD subjects (50 ± 20 years; 71 F) age and sex matched to 31 controls (45 ± 15 years; 18 F) underwent biplanar radiographic imaging with subsequent calculation of standing radiographic spinopelvic parameters. All subjects filled HRQOL questionnaires such as SF36 and ODI. ASD were further divided into 34 ASD-sag (with PT > 25° and/or SVA >5 cm and/or PI-LL >10°), 32 ASD-hyperTK (with only TK >60°), and 27 ASD-front (with only frontal malalignment: Cobb >20°). All subjects underwent 3D motion analysis during the sit-to-stand and stand-to-sit movements. The range of motion (ROM) and mean values of pelvis, lower limbs, thorax, head, and spinal segments were calculated on the kinematic waveforms. Kinematics were compared between groups and correlations to radiographic and HRQOL scores were computed. During sit-to-stand and stand-to-sit movements, ASD-sag had decreased pelvic anteversion (12.2 vs 15.2°), hip flexion (53.0 vs 62.2°), sagittal mobility in knees (87.1 vs 93.9°), and lumbar mobility (L1L3-L3L5: −9.1 vs −6.8°, all p < 0.05) compared with controls. ASD-hyperTK showed increased dynamic lordosis (L1L3–L3L5: −9.1 vs −6.8°), segmental thoracic kyphosis (T2T10–T10L1: 32.0 vs 17.2°, C7T2–T2T10: 30.4 vs 17.7°), and thoracolumbar extension (T10L1–L1L3: −12.4 vs −5.5°, all p < 0.05) compared with controls. They also had increased mobility at the thoracolumbar and upper-thoracic spine. Both ASD-sag and ASD-hyperTK maintained a flexed trunk, an extended head along with an increased trunk and head sagittal ROM. Kinematic alterations were correlated to radiographic parameters and HRQOL scores. Even after controlling for demographic factors, dynamic trunk flexion was determined by TK and PI-LL mismatch (adj. R2 = 0.44). Lumbar sagittal ROM was determined by PI-LL mismatch (adj. R2 = 0.13). In conclusion, the type of spinal deformity in ASD seems to determine the strategy used for sitting and standing. Future studies should evaluate whether surgical correction of the deformity could restore sitting and standing kinematics and ultimately improve quality of life.
Human Weight Compensation With a Backdrivable Upper-Limb Exoskeleton: Identification and Control
Authors: Verdel Dorian , Bastide Simon , Vignais Nicolas , Bruneau Olivier , Berret Bastien
Published: January 2022

Active exoskeletons are promising devices for improving rehabilitation procedures in patients and preventing musculoskeletal disorders in workers. In particular, exoskeletons implementing human limb’s weight support are interesting to restore some mobility in patients with muscle weakness and help in occupational load carrying tasks. The present study aims at improving weight support of the upper limb by providing a weight model considering joint misalignments and a control law including feedforward terms learned from a prior population-based analysis. Three experiments, for design and validation purposes, are conducted on a total of 65 participants who performed posture maintenance and elbow flexion/extension movements. The introduction of joint misalignments in the weight support model significantly reduced the model errors, in terms of weight estimation, and enhanced the estimation reliability. The introduced control architecture reduced model tracking errors regardless of the condition. Weight support significantly decreased the activity of antigravity muscles, as expected, but increased the activity of elbow extensors because gravity is usually exploited by humans to accelerate a limb downwards. These findings suggest that an adaptive weight support controller could be envisioned to further minimize human effort in certain applications.
Diabetes Medication Metformin Inhibits Osteoclast Formation and Activity in In Vitro Models for Periodontitis
Authors: Tao Lucy Y. , Łagosz-Ćwik Katarzyna B. , Hogervorst Jolanda M.A. , Schoenmaker Ton , Grabiec Aleksander M. , Forouzanfar Tim , van der Weijden Fridus A. , de Vries Teun J.
Published: January 2022

Diabetes and periodontitis are comorbidities and may share common pathways. Several reports indicate that diabetes medication metformin may be beneficial for the periodontal status of periodontitis patients. Further research using appropriate cell systems of the periodontium, the tissue that surrounds teeth may reveal the possible mechanism. Periodontal ligament fibroblasts anchor teeth in bone and play a role in the onset of both alveolar bone formation and degradation, the latter by inducing osteoclast formation from adherent precursor cells. Therefore, a cell model including this type of cells is ideal to study the influence of metformin on both processes. We hypothesize that metformin will enhance bone formation, as described for osteoblasts, whereas the effects of metformin on osteoclast formation is yet undetermined. Periodontal ligament fibroblasts were cultured in the presence of osteogenic medium and 0.2 or 1 mM metformin. The influence of metformin on osteoclast formation was first studied in PDLF cultures supplemented with peripheral blood leukocytes, containing osteoclast precursors. Finally, the effect of metformin on osteoclast precursors was studied in cultures of CD14+ monocytes that were stimulated with M-CSF and receptor activator of Nf-κB ligand (RANKL). No effects of metformin were observed on osteogenesis: not on alkaline phosphatase activity, Alizarin red deposition, nor on the expression of osteogenic markers RUNX-2, Collagen I and Osteonectin. Metformin inhibited osteoclast formation and accordingly downregulated the genes involved in osteoclastogenesis: RANKL, macrophage colony stimulating factor (M-CSF) and osteoclast fusion gene DC-STAMP. Osteoclast formation on both plastic and bone as well as bone resorption was inhibited by metformin in M-CSF and RANKL stimulated monocyte cultures, probably by reduction of RANK expression. The present study unraveling the positive effect of metformin in periodontitis patients at the cellular level, indicates that metformin inhibits osteoclast formation and activity, both when orchestrated by periodontal ligament fibroblasts and in cytokine driven osteoclast formation assays. The results indicate that metformin could have a systemic beneficiary effect on bone by inhibiting osteoclast formation and activity.
New Insights into LINC00346 and its Role in Disease
Authors: Lu Juan , Xiao Zhaoying , Xu Mengqiu , Li Lanjuan
Published: January 2022

Accumulating evidence has shown that long intergenic non-protein-coding RNA 346 (LINC00346) functions as an oncogene in the tumorigenesis of several cancers. The expression level of LINC00346 has been shown to be obviously correlated with prognosis, lymphoma metastasis, histological grade, TNM stage, tumor size and pathologic stage. LINC00346 has been found to regulate specific cellular functions by interacting with several molecules and signaling pathways. In this review, we summarize recent evidence concerning the role of LINC00346 in the occurrence and development of diseases. We also discuss the potential clinical utility of LINC00346, thereby providing new insight into the diagnosis and treatment of diseases. In addition, we further discuss the potential clinical utility of LINC00346 in the diagnosis, prognostication, and treatment of diseases.
Solventless Microextration Techniques for Pharmaceutical Analysis: The Greener Solution
Authors: Mohamed Heba M.
Published: January 2022

Extensive efforts have been made in the last decades to simplify the holistic sample preparation process. The idea of maximizing the extraction efficiency along with the reduction of extraction time, minimization/elimination of hazardous solvents, and miniaturization of the extraction device, eliminating sample pre- and posttreatment steps and reducing the sample volume requirement is always the goal for an analyst as it ensures the method’s congruency with the green analytical chemistry (GAC) principles and steps toward sustainability. In this context, the microextraction techniques such as solid-phase microextraction (SPME), stir bar sorptive extraction (SBSE), microextraction by packed sorbent (MEPS), fabric phase sorptive extraction (FPSE), in-tube extraction dynamic headspace (ITEX-DHS), and PAL SPME Arrow are being very active areas of research. To help transition into wider applications, the new solventless microextraction techniques have to be commercialized, automated, and validated, and their operating principles to be anchored to theory. In this work, the benefits and drawbacks of the advanced microextraction techniques will be discussed and compared, together with their applicability to the analysis of pharmaceuticals in different matrices.
Silica Nanochannel Array Film Supported by ß-Cyclodextrin-Functionalized Graphene Modified Gold Film Electrode for Sensitive and Direct Electroanalysis of Acetaminophen
Authors: Zhou Huaxu , Ding Yao , Su Ruobing , Lu Dongming , Tang Hongliang , Xi Fengna
Published: January 2022

Convenient and sensitive detection of active analytes in complex matrix is crucial in biological, medical, and environmental analysis. Silica nanochannel array film (SNF) equipped electrochemical sensors have shown excellent anti-fouling performance in direct analysis of complex samples. In this work, we demonstrated an electrochemical sensor with anti-fouling performance for highly sensitive detection of acetaminophen (APAP) based on SNF supported by ß-cyclodextrin-graphene (CDG) nanocomposite modified Au film electrode (AuF). Because of their rich surface hydroxyls and 2D lamellar structure, CDG on AuF can serve as the nanoadhesive for compact binding SNF, which can be grown by electrochemical assisted self-assembly method in a few seconds. Attributable to the electrocatalytic property of graphene and the synergistic enrichment from both CD and SNF nanochannels towards analyte, the SNF/CDG/AuF sensor demonstrates sensitive detection of acetaminophen ranged from 0.2 to 50 μM with an ultralow limit-of-detection of 14 nM. Taking advantage of the anti-fouling ability of SNF, the sensor is able to realize accurate and convenient analysis of APAP in commercially available paracetamol tablets.
Endoplasmic Reticulum-Shaping Atlastin Proteins Facilitate KSHV Replication
Authors: Long Wen-ying , Zhao Guo-hua , Wu Yao
Published: January 2022

Kaposi’s sarcoma-associated herpesvirus (KSHV) has two life cycle modes: the latent and lytic phases. The endoplasmic reticulum (ER) is the site for KSHV production. Furthermore, ER stress can trigger reactivation of KSHV. Little is known about the nature of the ER factors that regulate KSHV replication. Atlastin proteins (ATLs which include ATL1, ATL2, and ATL3) are large dynamin-related GTPases that control the structure and the dynamics of the ER membrane. Here, we show that ATLs can regulate KSHV lytic activation and infection. Overexpression of ATLs enhances KSHV lytic activation, whereas ATLs silence inhibits it. Intriguingly, we find that silencing of ATLs impairs the response of cells to ER stress, and ER stress can promote the lytic activation of KSHV. Our study establishes that ATLs plays a critically regulatory role in KSHV infection, thus expanding the known scope of biological processes controlled by ATLs to include KSHV infection.
mazEF Homologue Has a Minor Role in Staphylococcus epidermidis 1457 Virulence Potential
Authors: Gaio Vânia , Lima Tânia , Vilanova Manuel , Cerca Nuno , França Angela
Published: January 2022

Staphylococcus epidermidis biofilm cells are characterized by increased antimicrobial tolerance and improved ability to evade host immune system defenses. These features are, in part, due to the presence of viable but non-culturable (VBNC) cells. A previous study identified genes potentially involved in VBNC cells formation in S. epidermidis biofilms, among which SERP1682/1681 raised special interest due to their putative role as a toxin–antitoxin system of the mazEF family. Herein, we constructed an S. epidermidis mutant lacking the mazEF genes homologues and determined their role in (i) VBNC state induction during biofilm formation, (ii) antimicrobial susceptibility, (iii) survival in human blood and plasma, and (iv) activation of immune cells. Our results revealed that mazEF homologue did not affect the proportion of VBNC cells in S. epidermidis 1457, refuting the previous hypothesis that mazEF homologue could be linked with the emergence of VBNC cells in S. epidermidis biofilms. Additionally, mazEF homologue did not seem to influence key virulence factors on this strain, since its deletion did not significantly affect the mutant biofilm formation capacity, antimicrobial tolerance or the response by immune cells. Surprisingly, our data suggest that mazEF does not behave as a toxin–antitoxin system in S. epidermidis strain 1457, since no decrease in the viability and culturability of bacteria was found when only the mazF toxin homologue was being expressed.
Propionibacterium (Cutibacterium) granulosum Extracellular DNase BmdE Targeting Propionibacterium (Cutibacterium) acnes Biofilm Matrix, a Novel Inter-Species Competition Mechanism
Authors: Bronnec Vicky , Eilers Hinnerk , Jahns Anika C. , Omer Hélène , Alexeyev Oleg A.
Published: January 2022

Acne vulgaris is the most common dermatological disorder worldwide affecting more than 80% of adolescents and young adults with a global prevalence of 231 million cases in 2019. The involvement of the skin microbiome disbalance in the pathophysiology of acne is recognized, especially regarding the relative abundance and diversity of Propionibacterium acnes a well-known dominant human skin commensal. Biofilms, where bacteria are embedded into a protective polymeric extracellular matrix, are the most prevalent life style for microorganisms. P. acnes and its biofilm-forming ability is believed to be a contributing factor in the development of acne vulgaris, the persistence of the opportunistic pathogen and antibiotic therapy failures. Degradation of the extracellular matrix is one of the strategies used by bacteria to disperse the biofilm of competitors. In this study, we report the identification of an endogenous extracellular nuclease, BmdE, secreted by Propionibacterium granulosum able to degrade P. acnes biofilm both in vivo and in vitro. This, to our knowledge, may represent a novel competitive mechanism between two closely related species in the skin. Antibiotics targeting P. acnes have been the mainstay in acne treatment. Extensive and long-term use of antibiotics has led to the selection and spread of resistant bacteria. The extracellular DNase BmdE may represent a new bio-therapeutical strategy to combat P. acnes biofilm in acne vulgaris.
A Combination of Chest Radiography and Estimated Plasma Volume May Predict In-Hospital Mortality in Acute Heart Failure
Authors: Kobayashi Masatake , Douair Amine , Coiro Stefano , Giacomin Gaetan , Bassand Adrien , Jaeger Déborah , Duarte Kevin , Huttin Olivier , Zannad Faiez , Rossignol Patrick , Chouihed Tahar , Girerd Nicolas
Published: January 2022

Background: Patients with heart failure (HF) often display dyspnea associated with pulmonary congestion, along with intravascular congestion, both may result in urgent hospitalization and subsequent death. A combination of radiographic pulmonary congestion and plasma volume might screen patients with a high risk of in-hospital mortality in the emergency department (ED).

Methods: In the pathway of dyspneic patients in emergency (PARADISE) cohort, patients admitted for acute HF were stratified into 4 groups based on high or low congestion score index (CSI, ranging from 0 to 3, high value indicating severe congestion) and estimated plasma volume status (ePVS) calculated from hemoglobin/hematocrit.

Results: In a total of 252 patients (mean age, 81.9 years; male, 46.8%), CSI and ePVS were not correlated (Spearman rho <0 .10, p > 0.10). High CSI/high ePVS was associated with poorer renal function, but clinical congestion markers (i.e., natriuretic peptide) were comparable across CSI/ePVS categories. High CSI/high ePVS was associated with a four-fold higher risk of in-hospital mortality (adjusted-OR, 95%CI = 4.20, 1.10-19.67) compared with low CSI/low ePVS, whereas neither high CSI nor ePVS alone was associated with poor prognosis (all-p-value > 0.10; Pinteraction = 0.03). High CSI/high ePVS improved a routine risk model (i.e., natriuretic peptide and lactate)(NRI = 46.9%, p = 0.02), resulting in high prediction of risk of in-hospital mortality (AUC = 0.85, 0.82-0.89).

Conclusion: In patients hospitalized for acute HF with relatively old age and comorbidity burdens, a combination of CSI and ePVS was associated with a risk of in-hospital death, and improved prognostic performance on top of a conventional risk model.
Case Report: Next Generation Sequencing in Clinical Practice–A Real Tool for Ending the Protracted Diagnostic Odyssey
Authors: Limonova Alena S. , Ershova Alexandra I. , Meshkov Alexey N. , Kiseleva Anna V. , Divashuk Mikhail G. , Kurkina Marina V. , Drapkina Oxana M.
Published: January 2022

We reported a case of sitosterolemia, which is a rare genetic disease, characterized by increased plant sterol absorption and great heterogeneity of clinical manifestations. Our patient was initially referred to the lipid clinic due to high cholesterol levels and premature cardiovascular disease. Diagnosis of familial hypercholesterolemia was established in accordance with the Dutch Lipid Clinic Network criteria. Next-generation sequencing was later performed, which revealed a nonsense mutation in the ABCG8 gene, which led to the diagnosis of sitosterolemia. The aim of our report is to demonstrate, how genetic testing helped to make the correct diagnosis and to explain many of the patient's health problems, which etiology remained unclear for many years.
Comparative Efficacy of Medical Treatments for Chronic Heart Failure: A Network Meta-Analysis
Authors: Xiang Boyang , Yu Zongliang , Zhou Xiang
Published: January 2022

Background: The medical treatments of chronic heart failure have made remarkable progress in recent years. It is crucial to determine the optimal drug combination based on current evidence.

Methods: A search of PubMed, EMBASE, and Cochrane CENTRAL databases was conducted for studies on angiotensin receptor-neprilysin inhibitors (ARNIs), sodium-glucose cotransporter 2 inhibitors (SGLT2is), angiotensin-converting enzyme inhibitors (ACEIs), angiotensin receptor blockers (ARBs), beta-blockers (BBs), mineralocorticoid receptor antagonists (MRAs), and ivabradine (IVA) between 1987 and 2021. The network meta-analysis was performed to compare the efficacy of drug therapies in heart failure with reduced ejection fraction (HFrEF).

Results: Forty-eight randomized controlled trials (RCTs), which overall included 68,074 patients with HF and left ventricular ejection fraction (LVEF) ≤ 40%, were identified and included in the network meta-analysis. The efficacies of 13 intervention classes, including monotherapies or combinations of ACEI, ARB, ARNI, BB, MRA, SGLT2i, IVA, and placebo, on hospitalization for HF, cardiovascular mortality, and all-cause mortality were compared. Among the 13 included interventions, ARNI+BB+MRA, SGLT2i+ACEI+BB+MRA, and IVA+ACEI+BB+MRA were found to be best in terms of all three outcomes. Compared with placebo, these three drug combinations were associated with significant reductions in the risk of all-cause death, cardiovascular mortality and hospitalization for HF.

Conclusions: ARNI+BB+MRA, SGLT2i+ACEI+BB+MRA, and IVA+ACEI+BB+MRA were the top three therapies for patients with HFrEF. The increasing use of combinations of conventional and novel drugs contributed to progressive reductions in hospitalization and mortality in patients with HFrEF.