bluuebunny/crossref_metadata_2025 huggingface dataset:
Multifaceted Role of Apolipoprotein C3 in Cardiovascular Disease Risk and Metabolic Disorder in Diabetes
Authors: Pan Bo-Yi , Chen Chen-Sheng , Chen Fang-Yu , Shen Ming-Yi
Published: November 2024

Apolipoprotein C3 (APOC3) plays a critical role in regulating triglyceride levels and serves as a key predictor of cardiovascular disease (CVD) risk, particularly in patients with diabetes. While APOC3 is known to inhibit lipoprotein lipase, recent findings reveal its broader influence across lipoprotein metabolism, where it modulates the structure and function of various lipoproteins. Therefore, this review examines the complex metabolic cycle of APOC3, emphasizing the impact of APOC3-containing lipoproteins on human metabolism, particularly in patients with diabetes. Notably, APOC3 affects triglyceride-rich lipoproteins and causes structural changes in high-, very low-, intermediate-, and low-density lipoproteins, thereby increasing CVD risk. Evidence suggests that elevated APOC3 levels—above the proposed safe range of 10–15 mg/dL—correlate with clinically significant CVD outcomes. Recognizing APOC3 as a promising biomarker for CVD, this review underscores the urgent need for high-throughput, clinically feasible methods to further investigate its role in lipoprotein physiology in both animal models and human studies. Additionally, we analyze the relationship between APOC3-related genes and lipoproteins, reinforcing the value of large-population studies to understand the impact of APOC3 on metabolic diseases. Ultimately, this review supports the development of therapeutic strategies targeting APOC3 reduction as a preventive approach for diabetes-related CVD.
Only Infant MLL-Rearranged Leukemia Is Susceptible to an Inhibition of Polo-like Kinase 1 (PLK-1) by Volasertib
Authors: Fischer Jacqueline , Erkner Estelle , Radszuweit Pia , Hentrich Thomas , Keppeler Hildegard , Korkmaz Fulya , Schulze-Hentrich Julia , Fitzel Rahel , Lengerke Claudia , Schneidawind Dominik , Schneidawind Corina
Published: November 2024

MLL-rearranged (MLLr) leukemia is characterized by a poor prognosis. Depending on the cell of origin, it differs in the aggressiveness and therapy response. For instance, in adults, volasertib blocking Polo-like kinase 1 (PLK-1) exhibited limited success. Otherwise, PLK-1 characterizes an infant MLLr signature, indicating potential sensitivity. By using our CRISPR/Cas9 MLLr model in CD34+ cells from human cord blood (huCB) and bone marrow (huBM) mimicking the infant and adult patient diseases, we were able to shed light on this phenomenon. The PLK-1 mRNA level was significantly increased in our huCB compared to the huBM model, which was underpinned by analyzing infant and adult MLLr leukemia patients. Importantly, the expression levels correlated with a functional response. Volasertib induced a significant dose-dependent decrease in proliferation and cell cycle arrest, most pronounced in the infant model. Mechanistically, upon volasertib treatment, we uncovered negative feedback only in the huBM model by compensatory upregulation of PLK-1 and related genes like AURKA involved in mitosis. Importantly, the poor response could be overcome by a combinatorial strategy with alisertib, an Aurora kinase A inhibitor. Our study emphasizes the importance of considering the cell of origin in therapeutic decision-making and provides the rationale for evaluating volasertib and alisertib in MLLr leukemia.
Association of Sympathovagal Imbalance with Increased Inflammation and Impaired Adaptive Immunity in Bladder Cancer Patients
Authors: Mikolaskova Iveta , Zvarik Milan , Szaboova Kinga , Tibenska Elena , Durmanova Vladimira , Suchankova Magda , Kollarik Boris , Hesko Patrik , Palacka Patrik , Bucova Maria , Hunakova Luba
Published: November 2024

Stress responses can impact bladder cancer (BC) outcomes via immune–inflammatory pathway modulation. This study explores heart rate variability (HRV) associations with serum immune–inflammatory biomarkers, blood count inflammatory markers, and psychosocial self-report measures in patients versus healthy controls. The TREM-1 and TREM-2 expressions on peripheral blood monocytes were analysed via flow cytometry; serum inflammatory biomarkers by ELISA; HRV (5-min ECG) pre-tumour resection; blood counts by haematology analyser; and psychosocial factors by validated questionnaires. Patients exhibited altered immune–inflammatory profiles with increased TREM-1/TREM-2, sTREM-1, sTREM-1/sTREM-2 ratio, BDNF, MCP-1, and NLR, and reduced IFN-γ, IL-10, LMR, and PMR. HRV analysis indicated sympathetic dominance (SNS, Stress indices, ACmod) and reduced parasympathetic modulation (PNS index, SDNN, RMSSD, 2UV%, DCmod, SD1). Sympathetic HRV indices correlated positively with sTREM-1, sTREM-1/sTREM-2 ratio, fractalkine, and inflammatory markers (SII, NLR, PLR) and negatively with parasympathetic HRV indices—correlations absent in controls. Only in patients, reduced physical function and social support, and higher anxiety, depression, and fatigue, associated positively with sympathetic HRV indices and inflammatory markers. This study links immune–inflammatory markers, HRV parameters, and psychosocial factors in BC, suggesting that immune and autonomic variations may relate to unfavourable outcomes. Incorporating these assessments could help tailor more personalised treatment strategies for BC patients.
New Species and Records of Lemophagus Townes, 1965 (Hymenoptera, Ichneumonidae, Campopleginae), from China
Authors: Han Yuanyuan , Wei Chengxue , Liu Chong , Dong Yan
Published: November 2024

Campopleginae, the third largest subfamily of Ichneumonidae, has been one of the most investigated ichneumonids from the perspective of biological control. Despite their importance as potential biological control agents, species of campoplegine are among the most poorly studied insect groups. In this study, one new species of the genus Lemophagus Townes, 1965, Lemophagus nanus sp. nov., is described and illustrated. In addition, L. curtus and L. pulcher are recorded from China for the first time.
Exercise, Sports, and Cardiac Rehabilitation Recommendations in Patients with Aortic Aneurysms and Post-Aortic Repair: A Review of the Literature
Authors: Stiefel Michael , Brito da Silva Hadassa , Schmied Christian Marc , Niederseer David
Published: November 2024

Introduction: Balancing the well-documented benefits of regular exercise, particularly its positive impact on cardiovascular risk factors like hypertension, with the potential risks for patients with aortic aneurysms presents a significant challenge. This narrative review aims to summarize the current evidence and guidelines to assist clinicians in making informed exercise and sports recommendations for patients with aortic aneurysms or post-aortic repair. Methods: Nine clinical trials on the effect of exercise on abdominal aortic aneurysms (AAAs) were identified, including one study on cardiopulmonary exercise testing (CPET) in AAA patients. As no clinical trials on exercise in thoracic aortic aneurysms (TAAs) were found, we extrapolated data from other studies on exercise in aortic diseases, including data from patients who have had an aortic dissection, as well as three studies on cardiac rehabilitation (CR) and one study on CPET after proximal aortic repair. Review articles and guidelines were also incorporated to ensure a comprehensive overview of the topic. Results: Currently, no clear correlation exists between intense sports activities and the development of aortic aneurysms or dissections. Conclusions: Light to moderate physical activity appears safe and beneficial for patients with aortic aneurysms and post-aortic repair. Given the lack of evidence linking athletic activity to aortic complications, caution is warranted in restricting such activities for athletes, underscoring the importance of shared decision-making. Regular follow-up and optimal management of cardiovascular risk factors are essential.
Enhanced Hypercoagulability Using Clot Waveform Analysis in Patients with Acute Myocardial Infarction and Acute Cerebral Infarction
Authors: Masuda Jun , Wada Hideo , Kato Takashi , Tanigaito Yusuke , Hayashi Koken , Yamada Keita , Nishida Keigo , Oizumi Hiroki , Kamon Toshitaka , Ohkubo Takanobu , Okamoto Karin , Ito Nobuo , Shiraki Katsuya , Ichikawa Yuhuko , Shimaoka Motomu , Dohi Kaoru , Shimpo Hideto
Published: November 2024

Background: Routine activated partial thromboplastin time (APTT) and prothrombin time (PT) measurements do not indicate hypercoagulability in patients with acute myocardial infarction (AMI) and acute cerebral infarction (ACI). Methods: Hypercoagulability in patients with AMI or ACI was evaluated using a clot waveform analysis of the APTT or a small amount of tissue factor activation assay (sTF/FIXa). In the CWA, the derivative peak time (DPT), height (DPH), width (DPW), and area the under the curve (AUC) were evaluated. Results: The APTT did not indicate hypercoagulability, but the second DPT of CWA-sTF/FIXa was significantly shorter in patients with ACI than in healthy volunteers (HVs). The first DPH values of CWA-APTT and CWA-sTF/FIXa in patients with ACI and AMI were significantly higher than in HVs. In the receiver operating characteristic (ROC) analyses of ACI or AMI vs. non-thrombosis, the AUC was >0.800 in the DPHs of CWA-APTT and CWA-sTF/FIXa. The AUC of CWA-APTT and CWA-sTF/FIXa in patients with AMI and ACI was significantly higher than in HVs. The AUC/second DPT of CWA-APTT and CWA-sTF/FIXa in patients with AMI and ACI was significantly higher than in HVs. Regarding the ROC analyses of ACI or AMI vs. HVs, the AUC of ROC was higher than 0.800 in the AUC and AUC/second DPT of CWA-APTT and CWA-sTF/FIXa. Conclusions: The AUC/second DPT of CWA-APTT and CWA-sTF/FIXa may be a useful parameter for detecting a hypercoagulable state in patients with AMI and ACI.
Efficacy and Safety of Janus Kinase-Inhibitors in Ulcerative Colitis
Authors: Neri Benedetto , Mancone Roberto , Fiorillo Mariasofia , Schiavone Sara Concetta , Migliozzi Stefano , Biancone Livia
Published: November 2024

Background: Janus kinase-inhibitors (JAK-i) have recently been approved for treating patients with Ulcerative Colitis (UC); therefore, further information is needed, particularly regarding efficacy and safety. Objectives: To provide a comprehensive review regarding the efficacy and safety of currently available JAK-i in UC. Methods: The PubMed and Scopus databases were considered, searching for ‘JAK’, ‘JAK-inhibitor’, ‘Janus Kinases’, ‘Tofacitinib’, ‘Filgotinib’, ‘Upadacitinib’, individually or in combination with ‘IBD’, ‘Ulcerative Colitis’, ‘safety’, ‘efficacy’, ‘study’ and ‘trial’. The search was focused on full-text papers published in English, with no publication date restrictions. Results: The efficacy and safety of JAK-i approved for treating patients with UC have been summarized. These included Tofacitinib, Filgotinib and Upadacitinib. Findings from both clinical trials and real-life studies in UC were reported, with particular regard to their efficacy in inducing clinical response and remission, steroid-free remission and endoscopic and histological healing. Overall, JAK-i proved to be effective and safe in selected subgroups of patients with UC. The rapid onset of action and the oral route of administration represent the most relevant characteristics of these drugs. Safety concerns using Tofacitinib in subgroups of patients (infections, hypercholesterolemia, venous thromboembolism and cardiovascular events) were initially raised. More recently, all JAK-i for UC showed an overall satisfactory safety profile. However, indication should be carefully given. Conclusions: The use of JAK-i UC is promising, although no predictive markers of response are currently available. Optimizing their use, as monotherapy or combined with other immunomodulators, may increase their efficacy in appropriately selected subgroups of patients with UC.
A Rare Case of Peripheral Osteoma of the Alveolar Bone of the Maxilla in a 13-Year-Old Boy
Authors: Gušić Ivana , Stojilković Marija , Mirnić Jelena , Veljović Tanja , Đurić Milanko
Published: November 2024

Background: This report aims to augment the presently limited knowledge on the characteristics of jawbone osteomas in children by presenting an exceptionally rare case of this tumor located on the buccal aspect of the alveolar process of the maxilla in a 13-year-old boy. Methods: A well-defined, painless, bony, hard, spherical enlargement on the maxillary alveolar ridge was identified and thoroughly evaluated through clinical examination, panoramic radiographs, CBCT (Cone Beam Computed Tomography) scans, and histopathological analysis. The tumor was surgically removed, and the patient participated in postoperative clinical follow-ups for eight years. Results: Based on the clinical characteristics and CBCT scan findings, a jawbone tumor was suspected. After histopathological analysis, the definitive diagnosis was a peripheral trabecular osteoma. There were no signs of tumor recurrence during the postoperative follow-up period. Conclusions: This report presents the youngest documented case of peripheral osteoma in the maxillary alveolar ridge, and highlights the need to consider this rare lesion in the differential diagnosis of similar pathological changes in this region, even in pediatric patients. The absence of clinical signs of recurrence over eight years of follow-up underscores the long-term stability and favorable prognosis of peripheral jawbone osteoma in children.
Ten-Year Trends in Otolaryngological Surgery Volumes and the Impact of Infection Prevention During the COVID-19 Pandemic—A National Study
Authors: Valajärvi Ville-Emil , Lamminmäki Satu , Lundberg Marie , Hafrén Lena
Published: November 2024

Background/Objectives: This register study elucidates the national trends in the otolaryngologic surgery volume in Finland over a ten-year period. In particular, we investigated whether the pandemic, which had a marked effect on communicable diseases, had an impact on infection-related ear, nose, and throat (ENT) procedures. For reference, we used noninfectious ENT procedures. Methods: The data of this study consisted of the volumes of different otolaryngological surgical procedures in Finland from 2012 to 2022. A linear regression model was applied to calculate long-term trends in surgery volumes. The annual predicted and observed surgical volumes of each procedure were compared. In addition, different procedures were compared based on whether they were mostly infection-related, non-infection-related, or both. Results: The results revealed that the pandemic altered the trends of many ENT procedures, and during the pandemic, infection-related surgeries declined more than non-infection-related surgeries did. The decline in infection-related surgery volumes seems to have lasted longer than the coronavirus disease 2019 (COVID-19) pandemic itself, as only tympanostomies and mastoidectomies of all the infection-related procedures included in this study have returned to the pre-pandemic trend. Some non-infection-related procedures and procedures with mixed indications also declined during the pandemic and sustained their reduction even in 2022. Conclusions: This study provides a nationwide insight into ENT surgery volumes in Finland over a ten-year period. Although causative reasoning cannot be conducted based on this study, it still provides a good indication of how the absence of respiratory viruses and multifactorial societal restriction measures could have a long-lasting effect on the epidemiology and management of many ENT diseases.
Effects of Anatomical Variation on Ganglion Cell and Nerve Fibre Layer Evaluation by Optical Coherence Tomography
Authors: Dabbah Sami , Bjerager Jakob , Belmouhand Mohamed , Rothenbuehler Simon P. , Munch Inger Christine , Kolko Miriam , Larsen Michael
Published: November 2024

Background/Objectives: The automated analyses of optical coherence tomography (OCT) scans of the retina occasionally suggest the presence of tissue deficits when no visual field defects can be detected. This study was made to find the sources of such alerts. Methods: Data from a population-based cohort of 360 participants aged 30–80 years was analysed for the anatomical sources of alerts after the extensive exclusion of participants where any suspicion of abnormality could be raised. An analysis was made of 12 × 9 mm volume scans centred between the disc and the fovea. The exclusions comprised 107 eyes with definite or borderline abnormal visual fields or other potentially confounding characteristics. A statistical analysis of the thickness patterns was made using the manufacturer’s proprietary algorithm. The analysis comprised alerts corresponding to local layer thickness values in the lower 5th percentile of an independent reference population. Results: Of the 613 eligible healthy eyes, thickness deficit alerts were seen in 391. They were related to the angle between the temporal nerve fibre ridges being wider, narrower, or rotated compared to the reference template in 174 eyes and to the variations in the size of the macula in 207 eyes. The source was unidentifiable in 28 eyes. The common sources were a thin papillomacular nerve fibre layer accompanied by arcuate nerve fibre ridges spaced far apart and a thinly, but wider than the normal macular ganglion cell layer. Conclusions: Anatomical variation in the retinal nerve fibre and ganglion cell layers was the source of more than 90% of the thickness deficit alerts in the eyes with normal visual fields.
Prognostic Factors in Severe Eosinophilic Asthma in a Pediatric Population: A Prospective Cohort Study in Spain
Authors: Padró-Casas Clara , Basagaña María , Martínez-Colls María del Mar , García-Olivé Ignasi , Pollan Guisasola Carlos , Teniente-Serra Aina , Martínez-Cáceres Eva , Navarro José Tomás , Martínez-Rivera Carlos
Published: November 2024

Background/Objectives: The objective of this study was to provide real-world data on prognostic factors in children with severe eosinophilic asthma and to assess biomarkers of outcome. Methods: Fifty-nine children (aged 6–17 years) were included in a prospective cohort attended in a Severe Asthma Unit of a tertiary care teaching hospital in Badalona (Barcelona, Spain) and visited at baseline and at 1-year follow-up. Study variables included asthma control using the Asthma Control Test (ACT), forced expiratory volume in one second (FEV1), exacerbation episodes, fractional exhaled nitric oxide (FeNO), and inflammatory biomarkers (blood tests, sputum cells, immunoallergic tests, and levels of cytokines and effector cells in blood and sputum). Results: There were 36 boys and 23 girls, with a mean (SD) age of 11.9 (2.8) years. Uncontrolled severe asthma was diagnosed in 83.1% of cases, with poor symptom control (ACT score < 20) in 52.5%, obstructive pattern (FEV1 < 80% predicted) in 35.6%, and more than one exacerbation in the previous year in 30.5%. The mean duration of asthma was 9.2 (3.6) years. Positive prick tests were recorded in 55 patients, with polysensitization in 6. The mean percentage of sputum eosinophils was 2.5% (3.1%), and the mean eosinophil blood count 543.4 (427.7) cells/µL. Ten patients (32%) showed sputum eosinophilia (>3% eosinophils). Sputum eosinophils did not correlate with blood eosinophils, FeNO, and serum periostin. At 12 months, 13 (22%) children had uncontrolled asthma and 46 (78%) had controlled asthma. Variables significantly associated with uncontrolled asthma were duration of asthma (OR = 1.23, 95% CI 1.01–1.49, p = 0.04) and an ACT score < 20 (OR = 0.80, 95% CI 0.69–0.93, p = 0.004). Lower serum levels of IL-9 appeared to be related with uncontrolled asthma, but statistical significance was not reached. Conclusions: Pediatric severe eosinophilic asthma showed a predominant allergic phenotype with symptomatic disease as a main contributor of uncontrolled asthma at 1 year. Predictive biomarkers of outcome were not identified. Further studies are needed to confirm the present findings especially considering additional variables for a better phenotypic characterization of severe eosinophilic asthma in children and to study in-depth the role of inflammatory biomarkers.
The Role of Occlusal Appliances in Reducing Masseter Electromyographic Activity in Bruxism
Authors: Popescu Adrian Marcel , Vlăduțu Diana Elena , Ionescu Mihaela , Târtea Daniel Adrian , Popescu Sanda Mihaela , Mercuț Veronica
Published: November 2024

Background/Objectives: Bruxism is a masticatory muscle activity, phasic or tonic, with/without teeth contact, that appears in sleep or an awake state. An instrumental technique used to measure the surface electromyographic (sEMG) activity of the masseter muscle is used to diagnose bruxism activity during sleep and while awake. The objective of this study was to compare the variation in bruxism (sleep and awake) indices and masseter activity indices in low sleep bruxism and moderate sleep bruxism before and after wearing an occlusal appliance (OA) for 3 months each night. Methods: A clinical interventional study was designed in which subjects diagnosed with sleep bruxism were randomly selected to be included in the study. After the first sEMG recording, two groups were formed: a low sleep-bruxism group (number of sleep-bruxism events/h between 2 and 4) and a moderate sleep-bruxism group (number of sleep-bruxism events/h equal or higher than 4). All subjects received treatment with a 3D-printed occlusal appliance and wore it each night for 3 months, at which point the second sEMG recording was performed. For each participant of this study, a chart was created that included anamnestic data, clinical data, and sEMG data. The data were statistically analyzed with SPSS, using the Mann–Whitney U and Wilcoxon signed-rank tests. Results: A total of 21 participants were included in the final analysis, 18 women and 3 men, with a mean age of 24.5 ± 2.7 years. The OA lowered all bruxism indices in the whole group, but clusters analysis showed a significant reduction in sleep-bruxism indices in the moderate sleep-bruxism group, while in the low-bruxism group, the sleep and awake indices varied insignificantly, and the number of sleep-bruxism events/h remained constant. Conclusions: The 3D-printed occlusal appliances significantly lowered the sleep-bruxism indices and sleep masseter activity indices recorded with a portable sEMG device in the moderate sleep-bruxism group. The OA lowered the awake-bruxism indices and awake masseter activity indices in the moderate sleep-bruxism group.
Two New Species of Desmoscolex (Nematoda: Desmoscolecidae) from Subtidal Benthic Habitats in Korea, with a Comparative Analysis of Cephalic Setae Variability
Authors: Jung Wooin , Kihm Ji-Hoon , Rho Hyun Soo
Published: November 2024

This study describes two new species of the subgenus Desmoscolex (Nematoda: Desmoscolecidae) from subtidal benthic habitats in Korea, located in the Northwestern Pacific Ocean. Both species are distinguished by the absence of cephalic setae—a rare feature previously documented in only three species within this subgenus: Desmoscolex (Desmoscolex) rostratus Timm, 1970; D. (D.) asetosus Decraemer, 1975; and D. (D.) obscurus Bussau, 1993. The new species exhibit distinct morphological features. Desmoscolex (D.) lanceosetatus sp. nov. is characterized by an asymmetrical, dorsally curved head; large amphidial fovea that extend to the posterior margin of the first main ring; and lance-shaped subdorsal setae. In females, the absence of subventral setae on the 14th ring deviates from the typical arrangement of 17 main rings. Desmoscolex (D.) rotundicephalus sp. nov. is identified by its globular head, oval amphidial fovea, and tapering subdorsal setae with an open tip. In females, the absence of subventral setae on the 14th ring further differentiates it from closely related species. This paper provides detailed morphological descriptions supported by scanning electron microscopy (SEM) and differential interference contrast (DIC) images. Additionally, it includes a comprehensive morphological comparison with previously described species, species diagnoses, and a pictorial key for identification.
Underwater Drag Reduction Failure of Superhydrophobic Surface Caused by Adhering Spherical Air Bubbles
Authors: Nie You , Weng Ding , Wang Jiadao
Published: November 2024

Underwater drag reduction using superhydrophobic surfaces is a promising method due to its simplicity and low energy consumption. However, most attempts to obtain drag reduction using superhydrophobic surfaces have failed. Explanations such as air layer or air bubble vanishment and surface roughness are proposed in the existing works. In this work, the drag increase caused by spherical air bubbles adhering to the superhydrophobic surface is reported, and the drag increase mechanism is revealed by numerical simulation. In the water tunnel and towing tank experiment, we found that the experimental samples exhibited drag increase around a specific velocity, and the recorded optical images showed that the superhydrophobic surfaces were adhered by spherical air bubbles. Through numerical simulation, we found that the spherical air bubbles not only reduced the frictional drag but also introduced pressure drag. The drag increase was produced when the introduced pressure drag exceeded the reduced frictional drag. This work might be helpful for the drag reduction application of the superhydrophobic surface.
Genomic Features of Taiwanofungus gaoligongensis and the Transcriptional Regulation of Secondary Metabolite Biosynthesis
Authors: Zhang Yadong , Wang Yi , Yuan Xiaolong , Zhang Hongling , Zheng Yuan
Published: November 2024

Fungal secondary metabolites (SMs) have broad applications in biomedicine, biocontrol, and the food industry. In this study, whole-genome sequencing and annotation of Taiwanofungus gaoligongensis were conducted, followed by comparative genomic analysis with 11 other species of Polyporales to examine genomic variations and secondary metabolite biosynthesis pathways. Additionally, transcriptome data were used to analyze the differential expression of polyketide synthase (PKS), terpene synthase (TPS) genes, and transcription factors (TFs) under different culture conditions. The results show that T. gaoligongensis differs from other fungal species in genome size (34.58 Mb) and GC content (50.72%). The antibiotics and Secondary Metabolites Analysis Shell (AntiSMASH) analysis reveals significant variation in the number of SM biosynthetic gene clusters (SMBGCs) across the 12 species (12–29), with T. gaoligongensis containing 25 SMBGCs: 4 PKS, 6 non-ribosomal peptide synthetase (NRPS), and 15 TPS clusters. The TgPKS1 gene is hypothesized to be involved in the biosynthesis of orsellinic acid or its derivatives, while TgPKS2 might catalyze the synthesis of 6-methylsalicylic acid (6MSA) and its derivatives. The TgTRI5 genes are suggested to synthesize tetracyclic sesquiterpene type B trichothecene compounds, while TgPentS may be involved in the synthesis of δ-cadinol, β-copaene, and α-murolene analogs or derivatives. Comparative genomic analysis shows that the genome size of T. gaoligongensis is similar to that of T. camphoratus, with comparable SMs. Both species share four types of PKS domains and five distinct types of TPS. Additionally, T. gaoligongensis exhibits a high degree of similarity to Laetiporus sulphureus, despite belonging to a different genus within the same family. Transcriptome analysis reveals significant variation in the expression levels of PKS and TPS genes across different cultivation conditions. The TgPKS1 and TgPKS4 genes, along with nine TgTFs, are significantly upregulated under three solid culture conditions. In contrast, under three different liquid culture conditions, the TgPKS3, TgTRI5-1, and TgTRI5-2 genes, along with twelve TgTFs, exhibit higher activity. Co-expression network analysis and TgTFs binding site prediction in the promoter regions of TgPKS and TgTPS genes suggest that TgMYB9 and TgFTD4 regulate TgPKS4 expression. TgHOX1, TgHSF2, TgHSF3, and TgZnF4 likely modulate TgPKS3 transcriptional activity. TgTRI5-1 and TgTRI5-5 expression is likely regulated by TgbZIP2 and TgZnF15, respectively. This study provides new insights into the regulatory mechanisms of SMs in T. gaoligongensis and offers potential strategies for enhancing the biosynthesis of target compounds through artificial intervention.
Survival Outcomes in Sinonasal Mucosal Melanoma: Systematic Review and Meta-Analysis
Authors: Rojas-Lechuga María Jesús , Jubés Sara , Molina-García Manuel , da Silva-Júnior Rui Milton Patricio , Sampieri Claudio , Langdon Cristóbal , Gras-Cabrerizo Juan Ramón , Bernal-Sprekelsen Manuel , Puig Susana , Alobid Isam
Published: November 2024

Background/Objectives: Sinonasal mucosal melanomas (SNMMs) are rare and aggressive malignancies with poor survival outcomes. Our systematic review and meta-analysis aim to evaluate overall survival (OS) rates in patients with SNMM; Methods: We conducted a systematic search, following PRISMA guidelines across PubMed, Web of Science (WOS), and citation searching for studies reporting survival and prognosis outcomes for SNMMs. Inclusion criteria included studies with 5-year OS rates. Studies were excluded if they included tumor sites other than the paranasal sinuses or nasal cavity, were published in languages other than English and Spanish, or had a sample size of fewer than 15 patients. Two reviewers independently screened studies, extracted data, and assessed study quality using the Joanna Briggs Institute (JBI) critical appraisal checklist. Analyses of survival probabilities were conducted. Meta-analyses were performed using a random-effects model. PROSPERO ID CRD42024565137; Results: A total of 515 articles were identified after removing duplicates, and 99 reports were assessed for eligibility. Of these, 35 studies were included in the meta-analysis, encompassing a total of 2383 SNMM patients, of whom 1192 (50%) were female, with a weighted mean age of 65.4 years (SD = 5.4). Fifteen studies were from Europe (42.9%), six (17.1%) were from America, eleven (31.4%) were from Asia, two (5.7%) were from Australia, and one (2.9%) combined European, United Kingdom, and American populations. The 5-year OS was 34.8 [95% CI = 30.6–39.5], with the highest OS in America at 40.5 [95% CI = 34.1–48.1], followed by Europe at 36.6 [95% CI = 30.6–43.7], Australia at 32.3 [95% CI = 12.5–83.8], and Asia at 28.1 [95% CI = 19.5–40.7]. The age-standardized incidence rate (ASIR) for SNMM ranges was between 0.07 and 0.14 per 100,000 persons/year, with a slightly higher incidence in women than in men; Conclusions: This meta-analysis, one of the largest to date on SNMM, confirms the aggressive nature of this melanoma subtype with poor survival outcomes. Despite geographic differences in survival rates, the overall 5-year survival remains low, highlighting the urgent need for improved treatment strategies and more research to improve patient outcomes.
Exploring Biomarkers for Excess Extracellular Fluid in the Context of Physical Function in Chronic Kidney Disease Patients
Authors: Lee Hyae Min , Baek Jihyun , Lee So-Young , Lee Yu Ho , Jung Sang Hyun , Jeong Hye Yun
Published: November 2024

Background/Objectives: Fluid overload is an important risk factor for protein-energy wasting, which could lead to poor outcomes, such as higher morbidity and mortality, in patients with chronic kidney disease (CKD). This study aimed to validate the possible myokine as a biomarker of volume status in patients with non-dialysis CKD. Methods: In total, 151 patients with CKD were enrolled from a single medical center. Demographic data were collected via medical record review. Bioimpedance analysis was performed to measure body composition, and physical performance was assessed by measuring hand grip strength. Results: The physical performance of hand grip strength (21.9 ± 8.7 vs. 19.0 ± 10.1 kg, p = 0.233) and walking speed (1.05 ± 0.29 vs. 0.86 ± 0.52 m/s, p = 0.192) was higher in the low extracellular water/total body water (ECW/TBW) ratio group. Although higher, the median value of the brain-derived neurotrophic factor (BDNF) was not significant in the low ECW/TBW ratio group. Despite that, it had significant negative correlations with the ECW/TBW ratio in Pearson’s correlation analyses (r = −0.329, p < 0.001; r = −0.287, p < 0.001; and r = −0.238, p = 0.003). Linear regression analysis showed that the BDNF level had a significant negative relationship with the ECW/TBW ratio and significant associations even after multivariate analysis. Conclusions: Among myokines, BDNF had a significant negative relationship with the ECW/TBW ratio, suggesting that BDNF could be a possible biomarker for volume status in patients with non-dialysis CKD.
Vegetation Restoration Patterns Influence the Supply and Interrelations of Grassland Ecosystem Services in Karst Desertification Control
Authors: Song Shuzhen , Chen Xingyan , Song Yuehua , Chi Yongkuan
Published: November 2024

An appropriate vegetation restoration pattern is crucial for maintaining and enhancing ecosystem functions and services in karst rocky desertification control areas. However, it is still unclear whether different vegetation restoration patterns will aggravate the trade-off of grassland ecosystem services in this area. This study focuses on grassland ecosystems in the karst desertification control area, comparing artificial restoration measures (Dactylis glomerata single-species sowing grassland, DG; Lolium perenne single-species sowing grassland, LP; Lolium perenne + Trifolium repens mixed-species sowing grassland, LT) with natural restoration measures (NG). Seven ecosystem services (forage yield, soil retention, soil water conservation, carbon fixation and release, soil carbon storage, soil nutrient retention, and biodiversity conservation) as well as total ecosystem services were quantified using field monitoring data. The relationships between these services were evaluated through Spearman correlation analysis. The results showed that different vegetation restoration patterns significantly influenced the provisioning, regulating, and supporting services of the grassland ecosystem (p < 0.001). Three types of relationships were observed (trade-off, synergy, and neutral), but the trade-off relationship was not significant (p > 0.05). The total ecosystem service of LT (0.79) was significantly higher than that of NG (0.21), DG (0.36), and LP (0.41), with a significant synergy observed between soil nutrient conservation, forage yield, and carbon sequestration and oxygen release (p < 0.05). Therefore, LT is considered the best vegetation restoration practice for the karst rocky desertification control area compared with other patterns. This study provides theoretical guidance for vegetation restoration in degraded karst ecosystems.
Landscape Character Classification with a Deep Neural Network: A Case Study of the Jianghan Plain
Authors: Qin Wenke , Li Wenpeng , Zhang Zhuohao , Chen Weiya , Wan Min
Published: November 2024

Grounded in the theoretical and methodological frameworks of landscape character identification from the European Landscape Map (LANMAP) and landscape character assessment (LCA), this study developed an AI-based tool for landscape character analysis to classify the Jianghan Plain’s landscape more effectively. The proposed method leveraged a deep learning model, the artificial intelligence-based landscape character (AI-LC) classifier, along with specific naming and coding rules for the unique landscape character of the Jianghan Plain. Experimental results showed a significant improvement in classification accuracy, reaching 89% and 86% compared to traditional methods. The classifier identified 10 macro-level and 18 meso-level landscape character types within the region, which were further categorized into four primary zones—a lake network river basin, a hillfront terrace, surrounding mountains, and a lake network island hill—based on natural and social features. These advancements contributed to the theoretical framework of landscape character assessment, offering practical insights for landscape planning and conservation while highlighting AI’s transformative potential in environmental research and management.
Bleeding Symptoms in Pediatric Patients with Congenital FVII Deficiency and Correlation to Thrombin Generation Assay Parameters: A Single-Center Retrospective Analysis
Authors: Di Felice Giovina , Iavarone Sonia , Montemari Anna Lisa , Tripiciano Costanza , Massoud Michela , Pezzi Simona , Giorni Chiara , Porzio Ottavia , Luciani Matteo
Published: November 2024

Inherited factor VII deficiency is the most common rare bleeding disorder, affecting about 1/500,000 individuals without gender predilection. Most of the patients with FVII 20–50% are asymptomatic, but post-traumatic or post-surgical bleeding may often occur since there is not an exact correlation between FVII plasma levels and the bleeding phenotype. We enrolled 19 children and adolescents with FVII levels of 20–35% and 33 controls. Laboratory data collected included thrombin generation, prothrombin time, activated partial thromboplastin time, fibrinogen, and FVII levels. In our study, we found a statistical difference in the lag time ratio (p < 0.01) and tt-peak ratio (p < 0.05) between patients and controls but no difference in the other parameters, such as the endogenous thrombin potential (ETP). However, when we categorized patients, regardless of their bleeding scores, as presenting symptoms and having no symptoms, both the lag time ratio (p = 0.01) and tt-peak ratio (p < 0.05) were significantly different, and the vel. index % showed increased levels in patients without symptoms (p < 0.05). This study shows that thrombin generation may be a useful tool in assessing the risk of bleeding symptoms in children with an FVII deficiency categorized in the mild category (20–35%), although we cannot predict the severity of the bleeding.
Towards a Model-Based Methodology for Rating and Monitoring Wear Risk in Oscillating Grease-Lubricated Rolling Bearings
Authors: Bartschat Arne , Stammler Matthias , Wenske Jan
Published: November 2024

Oscillating grease-lubricated slewing bearings are used in several applications. One of the most demanding and challenging is the rotor blade bearings of wind turbines. They allow the rotor blades to be turned to control the rotational speed and loads of the complete turbine. The operating conditions of blade bearings can lead to lubricant starvation of the contacts between rolling elements and raceways, which can result in wear damages like false brinelling. Variable oscillating amplitudes, load distributions, and the grease properties influence the likelihood of wear occurrence. Currently, there are no methods for rating this risk based on existing standards. This work develops an empirical methodology for assessing and quantifying the risk of wear damage. Experimental results of small-scale blade bearings show that the proposed methodology performs well in predicting wear damage and its progression on the raceways. Ultimately, the methods proposed here can be used to incorporate on-demand lubrication runs of pitch bearings, which would make turbine operation more reliable and cost-efficient.
Investigating the Anti-Wear Behavior of Polyalphaolefin Oil with Methyl Silicon Resin Using Advanced Analytical Techniques
Authors: Wang Haiyang , He Zhongyi , Xiong Liping , Qian Liang , Li Lili , Long Qiyang
Published: November 2024

This research thoroughly examined the tribological characteristics of polyalphaolefin (PAO4) oil, both with and without the incorporation of methyl silicone resin. The evaluation of anti-wear properties and friction reduction was conducted using a four-ball tester for friction and wear. The incorporation of methyl silicone resin into PAO4 at 25 °C significantly reduced the wear scar diameter (WSD), achieving minimum values at a concentration of 0.02 wt.%. PAO4 with 0.02 wt.% methyl silicone resin shows excellent wear resistance at different temperatures. A detailed analysis of the wear scar surfaces and wear debris was conducted using scanning electron microscope (SEM), energy dispersive spectrometer (EDS), X-ray photoelectron spectroscopy (XPS), Raman spectroscopy, 3D surface profiler and TEM. The results compellingly demonstrate that the remarkable improvement in wear resistance is predominantly due to the strategic formation of SiO2 nanoparticles during the friction process. These SiO2 particles not only adeptly fill the surface gaps at the friction interface but also crucially contribute to the formation of a robust tribochemical film, which is instrumental in enhancing wear performance.
Performance of Crumb Rubber Tire-Modified Bitumen for Malaysian Climate Regions
Authors: Blab Ronald , Ahmad Juraidah , Shaffie Ekarizan , Sidek Norbaya , Mirwald Johannes , Eberhardsteiner Lukas , Hofko Bernhard
Published: November 2024

Researchers are increasingly concerned about the vast amounts of waste rubber tires produced globally, which contribute significantly to environmental pollution. The potential of incorporating waste rubber tires to modify bitumen has garnered considerable interest. This study assesses pavement design temperatures according to SUPERPAVE standards for representative Malaysian regions. The assessment is based on hourly air temperature data and simulates temperature diffusion in typical Malaysian road pavements using the finite difference method (FDM). Tests on neat bitumen (PEN 60/70) and crumb rubber-modified bitumen (CR-TMB) samples evaluated their physical and rheological properties across various temperatures and aging stages. These tests were conducted using the dynamic shear rheometer, rotational viscometer, and bending beam rheometer. The attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) analysis provided insights into the aging processes of both PEN 60/70 and CR-TMB. The findings indicate that adding 15% crumb rubber to produce CR-TMB enhances the physical and rheological properties of bitumen. Additionally, this modification significantly improves aging behavior, highlighting its potential for more resilient and sustainable road construction materials. Therefore, the use of crumb rubber in road construction should be considered to improve pavement durability and strength. Furthermore, utilizing crumb rubber as an alternative material can reduce costs by recycling waste materials.
Formation of the Cu+Nb Interlayer in the Inconel 718/Ti6Al4V Multi-Material Obtained by Selective Laser Melting
Authors: Repnin Arseniy , Borisov Evgenii , Popovich Anatoly
Published: November 2024

This study examines the Inconel 718/Ti6Al4V multi-material with a Cu and Nb interlayer produced by SLM. To achieve this, it is necessary to investigate the microstructure, the chemical and phase composition, and the hardness of the interfacial zone in the multi-material samples. Furthermore, it is necessary to determine the impact of interlayer utilization on the mechanical properties of multi-material samples. The investigation showed that the formation of island macro-segregation was observed in all interfacial zones of the multi-material samples. The interfacial zones, Ti6Al4V/Nb and Cu/Inconel 718, exhibited a relatively sharp transition in the chemical composition. In contrast, the Cu/Nb interfacial zone exhibited a gradual transition. The results of the chemical composition study indicated that the width of the Nb/Cu transition zone was approximately 700 μm. No new phases were identified in the production of the multi-material samples. The typical phases were present in the alloy zone, as well as in the Nb/Cu interfacial zone. During the transition from the Ti6Al4V zone to the Inconel 718 zone through the Nb and Cu zones, the average microhardness values changed as follows: 270 → 190 → 120 → 300 HV. The ultimate tensile strength values for the multi-material samples reached 910 MPa.
The Coloring Mechanism of Opaque Glazes from the Changsha Kiln of the Tang Dynasty
Authors: Jia Lala , Zhang Maolin , Yu Yongqiang , Li Zihan , Yu Yongbin
Published: November 2024

The Changsha Kiln of the Tang Dynasty was a major site for celadon production, yet the mechanisms behind the unique coloring of its opaque glazes remain inadequately explored. Prior research has largely focused on the general composition of these glazes, with limited understanding of the specific processes contributing to their distinct visual characteristics. This gap in knowledge hinders efforts to accurately replicate these historical glazes and fully appreciate their technological significance. In this study, we analyze the chemical composition and microstructure of opaque glaze specimens from the Changsha Kiln using advanced techniques such as EDXRF, SEM-EDS, and ultra-deep field microscopy. Our findings reveal that the opaque glazes are high-calcium compositions where phase separation and the presence of coloring ions like Cu2+, Cu+, and Fe3+ play critical roles in determining the glaze’s color. The interplay between chemical coloring and phase separation processes is shown to produce the distinct blue-green and creamy white hues observed in the glazes. These results provide a deeper understanding of the coloring mechanisms in Changsha Kiln glazes, contributing to the broader field of ceramic research and aiding in the preservation and accurate reproduction of these historic artifacts.
Dynamic Mechanical Properties and Damage Morphology Analysis of Concrete with Different Aggregates Based on FDM-DEM Coupling
Authors: Liu Kaixuan , Chen Zhenfu , Tao Qiuwang , Wu Dan , Wu Qiongfang , Zou Pinyu , Wang Minghui , Li Yangzi
Published: November 2024

To study the dynamic compressive mechanical properties of concrete with different aggregates (limonite and lead-zinc ore), a dynamic mechanical experiment was carried out by the Φ 100 mm SHPB equipment. Based on the coupling of the finite difference method (FDM) and the discrete element method (DEM), a three-dimensional numerical model was constructed. The effects of various strain rates and aggregate types on the dynamic mechanical properties of concrete, the dynamic increase factor (DIF), and the dynamic impact damage process were analyzed and discussed. The results show that both types of concrete have a significant strain rate strengthening effect. The dynamic compressive strength, peak strain, and DIF of the two types of concrete gradually increase with the increasing strain rate. The DIF and dynamic compressive strength growth of lead-zinc ore concrete was greater than that of limonite concrete, and the strain rate sensitivity of lead-zinc ore concrete was stronger than that of limonite concrete. The constructed three-dimensional coupling model can better simulate the experimental process, and the stress-strain curves and damage patterns show good agreement with the experimental results. The relative errors between the calibration results of the microscopic parameters and the experiment values are all within 1%.
The Distributions and Dependences of 3D Particle Morphology Characteristics for Crushed and Natural Sands by X-Ray uCT Investigations
Authors: Yang Hao , Li Xu , Zhang Junhui , Hu Zhengbo , Li Shengnan
Published: November 2024

The morphology of an individual particulate refers to its shape characteristics and size properties, which both play important roles for granular matter in physics, mechanics, chemistry, and biology. In this study, ellipsoidality is defined as a 3D shape index for evaluating particle roundness, and an explicit calculation method is applied. The dependences of 3D shape characteristics (aspect ratios, sphericity, and ellipsoidal degree) on particle size (ranges from 0.063 mm to 5.0 mm) are adequately investigated with the X-ray micro-computed microtomography (uCT) imaging for hundreds of thousands of particles of crushed and natural sands. This study focuses on comparing and evaluating the specific surface area and equivalent diameter, suggesting that particle segregation and changes in surface area may explain the strong dependence of particle shape on size. The correlation between different shape metrics was analyzed by comparing crushed sand with natural sand to provide theoretical support for material filling and mechanical behaviour. The significant differences in the microscale particle size indexes of different sands by single grading are used to provide data references for further analyses of the effect of material microscale on material properties in future discrete element particle simulations.