bluuebunny/crossref_metadata_2025 huggingface dataset:
Investigating the relationship between diet quality, lifestyle and healthy eating index with severity and migraine attacks: a cross-sectional study
Authors: Feyzpour Marziye , Sedgi Fatemeh Maleki , Baghdadi Ghazal , Mohammadifard Reza , Rahimlou Mehran
Published: December 2024

Background

Migraine is a disabling neurovascular disorder often associated with comorbidities such as mental health disorders, cardiovascular diseases, and metabolic syndromes. While certain dietary triggers have been identified, the impact of overall diet quality on migraine severity and frequency is not well understood. This study aimed to evaluate the association between diet quality, lifestyle factors, and the Healthy Eating Index (HEI) with migraine severity and frequency.

Methods

A cross-sectional study was conducted on 280 patients aged 18–50 years newly diagnosed with migraines. Dietary intake was assessed using a 147-item Food Frequency Questionnaire (FFQ), and diet quality was evaluated using the Lifelines Diet Score (LLDS) and HEI. Migraine-related disability and severity were assessed using the Migraine Disability Assessment (MIDAS) questionnaire and the Visual Analogue Scale (VAS), respectively. Logistic regression models were applied to examine the association between diet quality and migraine outcomes.

Results

Higher LLDS and HEI scores were significantly associated with reduced odds of migraine-related disability. Participants in the highest LLDS tertile had an odds ratio (OR) of 0.68 (95% CI: 0.42–0.96; p = 0.02) for migraine disability. Similarly, the highest HEI tertile was associated with an OR of 0.58 (95% CI: 0.41–0.88; p = 0.025). For pain intensity, the highest tertile of LLDS showed an OR of 0.55 (95% CI: 0.38–0.75; p = 0.026), while the HEI showed an OR of 0.62 (95% CI: 0.45–0.85; p = 0.03).

Conclusion

Higher diet quality, as measured by LLDS and HEI scores, is inversely associated with migraine severity and frequency. These findings suggest that dietary improvements may be a viable strategy for managing migraine symptoms.
A diagnostic index for predicting heart rate variability decline and prognostic value in newly diagnosed non-small cell lung cancer patients
Authors: Zhang Lifang , Liu Ying , Han Di , Wang Yan , Geng Fanqi , Ding Wei , Zhang Xuejuan
Published: December 2024

Background

Heart rate variability (HRV) is an important marker of autonomic nervous system function and cardiovascular health. Holter monitoring is a crucial method for evaluating HRV, but the procedure and result analysis are relatively complex. This study aims to develop a simplified diagnostic index for predicting HRV decline in newly diagnosed non-small cell lung cancer (NSCLC) patients and evaluate its prognostic value.

Methods

This retrospective cross-sectional study included 131 newly diagnosed NSCLC patients. Baseline characteristics were compared between normal HRV group and declined HRV group. Univariate and multivariate logistic regression analyses identified significant predictors of HRV decline. A diagnostic index was developed based on resting heart rate (RHR), serum sodium, and interleukin-6 (IL-6) and externally validated. Kaplan-Meier survival analysis assessed the prognostic value of the index.

Results

Patients with declined HRV had higher median RHR (84 b.p.m. vs. 70 b.p.m., p < 0.001), lower serum sodium (136.3 mmol/L vs. 138.7 mmol/L, p < 0.001), lower serum albumin (39 g/L vs. 41 g/L, p = 0.031), higher lactate dehydrogenase (LDH) (202 U/L vs. 182 U/L, p = 0.010), and higher IL-6 (11.42 pg/ml vs. 5.67 pg/ml, p < 0.001). Multivariate analysis identified RHR (OR = 3.143, p = 0.034), serum sodium (OR = 6.806, p < 0.001), and IL-6 (OR = 3.203, p = 0.033) as independent predictors of HRV decline. The diagnostic index, with an area under the curve (AUC) of 0.849, effectively predicted HRV decline. ROC analysis of the external validation data demonstrated an AUC of 0.788. Survival analysis showed that patients with a diagnostic index > 2 had significantly worse overall survival (log-rank p < 0.001).

Conclusions

The study identified key clinical parameters that predict HRV decline in newly diagnosed NSCLC patients. The developed diagnostic index, based on RHR, serum sodium, and IL-6, effectively stratifies patients by HRV status and has significant prognostic value, aiding in early identification and management of high-risk patients.
Development of a new VMAT QA framework for Mobius3D using control-point specific EPID images
Authors: Seok JaeHyun , Kim Hojin , Han Min Cheol , Kim Jihun , Park Kwangwoo , Cho Hyeonjeong , Yoo Dohyeon , Kim Jin Sung
Published: December 2024

Purpose

This study presents novel quality assurance (QA) approach for volumetric modulated arc therapy (VMAT) that leverages frame-by-frame electronic portal imaging device (EPID) images integrated into Mobius3D for accurate three-dimensional dose calculations.

Methods

Sequential EPID images for VMAT plans were acquired every 0.4-second by iView system and processed through iterative deconvolution to mitigate blurring from photon scattering. Deconvolved images were binarized to define multi-leaf collimator (MLC) positions. Pre-acquired box fluences determined optimal threshold for binarization and adjusted for detector shift depending on gantry and collimator angles. Sequential EPID images were re-scaled using pixel scaling factor (PSF) and converted to monitor unit (MU) proportional values. Generated EPID-based log file, including control-point specific MLC and monitor units (MU) information, were analyzed in Mobius3D for Gamma passing rate (GPR) of VMAT plans from 18 patients. Plan complexity indices were calculated and correlated with GPR.

Results

Clinically appropriate threshold was defined to be 20000 that can extract accurate MLC data from the deconvolved binarized EPID images. Positional deviations due to gantry and collimator rotations were observed to be up to 4.5 pixels. Recalibrated EPID pixel values showed linearity with MU regardless of changes in dose rate. Consequently, average GPR for 18 patients evaluated using Mobius3D reached 95.2% ± 3.7%%, based on 3% dose difference and 3mm distance-to-agreement criterion. It was found that two plan complexity indices showed statistically significant correlation with GPR.

Conclusion

This study successfully implemented novel measurement-based VMAT QA framework based on control-point specific EPID, based upon accurate MLC and MU data at each frame.
Polyketides and alkaloids from the fungus Aspergillus Fumigatus YB4-17 and ent-Fumiquinazoline J induce apoptosis, paraptosis in human hepatoma HepG2 cells
Authors: Wang Huannan , Sun Lixiang , Ma Xueyang , Jin Shihao , Xi Yidan , Sai Chunmei , Yan Maocai , Cheng Zhongbin , Zhang Zhen
Published: December 2024

Hepatocellular carcinoma (HCC) is one of the most common malignancies. The currently available clinical drugs for HCC frequently cause serious side effects and the treatment outcomes are unsatisfactory. It is urgent to develop effective drugs with high selectivity and low adverse effects for HCC. Metabolites produced by microorganisms have shown great potential in the development of therapeutic agents for HCC. In our study, the EtOAc extract of the strain Aspergillus fumigatus YB4-17 exhibited significant cytotoxicity towards the HCC HepG2 cells at 10 μg/mL. Various column chromatographic separations of the extract afforded seven polyketides (1–7), including a new diphenyl ether derivative (1), along with fourteen known alkaloids (8–21). The structure elucidation was conducted via NMR spectroscopic data and MS data analysis. The absolute configuration of compound 11 was confirmed by comparing experimental and calculated electronic circular dichroism spectrum for the first time. The biological evaluation of these metabolites revealed that compound 11 selectively inhibited the proliferation of HCC HepG2 cells with negligible toxicity to normal cells. Mechanism study indicated that compound 11 induced apoptosis and paraptosis in HepG2 cells, providing a novel therapeutic perspective for the treatment of hepatocellular carcinoma.
Pemigatinib combined with immunotherapy and stereotactic body radiation therapy for FGFR2 fusion-positive advanced intrahepatic cholangiocarcinoma with brain metastasis: a Case Report
Authors: Guo Jiamin , Sun Lingqi , Chen Ye , Ma Ji
Published: December 2024

Background

FGFR2 fusions or rearrangements occur in 13%–20% of patients with intrahepatic cholangiocarcinoma (iCCA). Pemigatinib, a representative FGFR inhibitor, is commonly used for targeted therapy in such patients. Additionally, brain metastasis (BM) is extremely rare in advanced iCCA, and there is currently no standard treatment strategy for advanced iCCA patients with BM. Stereotactic body radiation therapy (SBRT) combined with immune checkpoint inhibitors (ICIs) may exhibit synergistic antitumor effects, presenting a promising approach for advanced iCCA.

Case presentation

The patient, a 58-year-old male, experienced a recurrence of iCCA following surgery and chemotherapy, with multiple metastases in the liver, lungs, and brain. Genetic testing revealed FGFR2-TXLNG-fusion, and the patient was treated with pemigatinib in combination with tislelizumab and SBRT for the BM, resulting in significant tumor shrinkage. Adverse events (AEs) such as liver dysfunction, nail loss, and dry mouth were observed during treatment, which were considered to be related to pemigatinib. These AEs were significantly alleviated after dose reduction and symptomatic treatment.

Conclusion

This case presented a rare occurrence of FGFR2 fusion-positive iCCA with BM, with extremely limited data on treatment options and survival outcomes in such patients. Our study was the first to report the application of the treatment strategy combining pemigatinib with ICI and SBRT in this specific case. The combined therapy proved effective and well-tolerated, providing new insights for future treatment considerations.
Turkish teachers’ values with rational and non-rational truth and teacher emotions in teaching
Authors: Ünal Kübra , Myyry Liisa , Toom Auli
Published: December 2024

The aim of this study was to clarify the relationships between personal values, truth-related values and emotions among Turkish teachers. The Schwartz’s Value Theory and Frenzel’s teacher emotion model were used as the theoretical framework. This study used a cross-sectional correlational research design. The data were collected from 279 teachers with the Portrait Value Questionnaire (PVQ) where rational truth and non-rational truth values were added, and with the Teacher Emotions Scale (TES). The circular structure of the Schwartz Value Theory was tested by multidimensional scaling. The data analysis aimed to uncover relationships between personal values, truth-related values, and emotions. Rational truth emerged near self-direction and self-transcendence, yet items measuring non-rational truth were scattered among values. Females had higher regard for self-direction and hedonism than males. Non-rational truth was negatively correlated with enjoyment, while it was positively correlated with anxiety. The implications for gender roles in Turkish society are discussed.
Alexithymia and estimated 10-year cardiovascular disease risk in healthy adults: a community-based cross-sectional study
Authors: Vadini Francesco , Lanzara Roberta , Iuliani Ornella , Affaitati Gianna Pia , Porcelli Piero
Published: December 2024

Background

This cohort study aimed to explore whether and to what extent alexithymia would be associated with cardiovascular disease (CVD) risk over an estimated 10-year period, over and above established clinical cofactors (i.e., depressive symptoms, quality of life, sociodemographic, anthropometric, lifestyle, and biological data), in a low-risk population of blood donors.

Methods

A sample of 1,021 adult Italian blood donors (age 46.9 ± 8.39; 61.2% men) was consecutively recruited. The 10-year-CVD risk score was estimated using the CUORE risk score (CRS). Sociodemographic, lifestyle, anthropometric, biological, and psychological (i.e., quality of life, depressive symptoms, and alexithymia) CVD risk data were assessed using validated self-report measures or clinical records.

Results

As expected, most participants (78.5%) had a low CVD risk (CRS < 3%) and an overall low-risk profile for all the parameters. Compared with subjects at low risk of CVD (n = 911, 78.5%), those with high risk (i.e., rated ≥3 on CUORE risk assessment; n = 250, 21.5%) reported higher levels of alexithymia (p < 0.001). Subjects with higher alexithymia (n = 236, 23.1%) reported higher levels of psychosocial impairment, depressive symptoms, and biological risk variables for CVD. Alexithymia was significantly associated with 10-year CVD risk (OR = 1.02, 95% CI = 1.01–1.04, p = 0.009), even after adjusting for key sociodemographic and clinical risk variables.

Conclusion

Although limited by the cross-sectional design, this study is the first to show that alexithymia leads to a higher risk for 10-year CVD estimate in healthy subjects with low-risk profile, regardless of known biomarkers and traditional CVD risk factors.
How is parenting stress related to parental burnout among children’s mothers in China: the mediating role of marital satisfaction and the moderating role of socioeconomic status
Authors: Xu Xiaoyan , Hanafi Zahyah , Zhang Shun
Published: December 2024

Introduction

Although parenting is a worthwhile and joyful process, it can also cause stress, potentially leading to parental burnout. With the implementation of the three-child policy in China, more parenting hours and higher economic costs may increase the risk of parental burnout.

Objectives

This study investigated how was maternal parenting stress related to mothers’ parental burnout, as well as the potential mediating effect of their marital satisfaction and the moderating effect of their socioeconomic status on this relationship.

Methods

Data were collected from 314 mothers living in mainland China. The Chinese versions of the Parenting Burnout Assessment Scale, Parenting Stress Index, and Marriage Perception Scale were used to measure mothers’ parental burnout, parenting stress, and marital satisfaction.

Results

Mothers‘ parenting stress was significantly and positively related to mothers’ parenting burnout. Mothers’ marital satisfaction mediated this relationship. Mothers’ socioeconomic status moderated the first half of the mediation model, and parenting stress exhibited a greater effect on marital satisfaction when mothers had a higher socioeconomic status.

Discussion

These findings indicated that mothers’ parenting stress could be alleviated by increasing marital satisfaction, which, in turn, reduced the risk of parental burnout. Furthermore, socioeconomic status may enhance the negative effects of parenting stress on marital satisfaction among mothers.
The dual process of prejudice: racism, nationalism, and sexism in the 2020 U.S. presidential election
Authors: Rusowicz Aleksandra , Pratto Felicia , Shook Natalie
Published: December 2024

Introduction

Modern racism, nationalism, and sexism have been proposed as major influences on contemporary U.S. politics. However, most work has not examined these interrelated factors together. Thus, it is unclear to what extent each form of prejudice uniquely contributes to political behavior. Furthermore, the potential motivations underlying the link between prejudice and politics have not been well elucidated. We sought to (1) determine the extent to which racism, sexism, and nationalism were uniquely associated with political outcomes in the 2020 U.S. presidential election and (2) use the dual-process motivational model to examine whether social dominance orientation (SDO) and right-wing authoritarianism (RWA) were potential motivations underlying the link between prejudice and political attitudes and behavior.

Methods

A national sample of U.S. adults (N = 531) completed online questionnaires before and after the 2020 U.S. election. Structural equation modeling was used to test mediational models in which SDO and RWA prospectively predicted presidential candidate evaluations and vote choice indirectly through racism, sexism, and nationalism.

Results

When examined in conjunction, modern racism (not sexism or nationalism) was consistently associated with evaluations of both candidates and vote choice. Furthermore, SDO and RWA both exerted indirect effects on candidate evaluations and vote choice through modern racism.

Discussion

These results are aligned with previous findings indicating that racism plays a unique role in U.S. politics and may be motivated by status threat experienced by some majority group members.
Modeling the electrical conductivity relationship between saturated paste extract and 1:2.5 dilution in different soil textural classes
Authors: Omar Moh’d M. , Shitindi Mawazo J. , Massawe Boniface H. J. , Pedersen Ole , Meliyo Joel L. , Fue Kadeghe G.
Published: December 2024

Regression models were developed to estimate the electrical conductivity of saturated paste extract (ECe) from the electrical conductivity of soil-water ratio (EC1:2.5) for different soil textural classes. ECe is a crucial parameter used to indicate the presence, type, and distribution of salinity in soils. However, determining ECe is demanding, time-consuming, requires considerable skill to accurately identify the correct soil saturation point, and is not routinely performed by soil testing laboratories. Many laboratories, instead, commonly measure the electrical conductivity of soil-water extracts at various dilutions, such as EC1:1, EC1:2.5, or EC1:5. In this study, 706 soil samples were collected from depths of 0 - 30 cm across three rice irrigation schemes to determine EC1:2.5, with 50% analyzed for ECe. ECe values were grouped based on soil textural classes. The results showed a strong linear relationship between EC1:2.5 and ECe values, with a high coefficient of determination (R² > 0.95). The Root Mean Square Error values were low (1.4 < RMSE), and the Mean Absolute Error values were similarly low (0.85 < MAE). Therefore, the regression models developed provide a practical means of estimating ECe for various soil textural classes, thereby enhancing soil salinity assessment and management strategies.
Adaptation to climate change among transhumant herders: evidences from northern Benin (West Africa)
Authors: Djakpa Milka Grâce , Egah Janvier , Degla Pamphile
Published: December 2024

Climate change and related effects such as water scarcity is a major issue for transhumant who need to develop sustainable adaptation strategies. We highlighted both the perceived climate change effects and the main socio-economic drivers of the decisions to use specific adaptation strategies. Our study used primary data collected through a survey approach from 100 transhumant randomly selected in two municipal areas in the northern of Benin. We used descriptive statistics and a multivariate regression model to analyze our data. Transhumant herders perceived climate change effects through variability of rainfall, of temperature and of wind direction. In response to the perceived changes, they adopted several strategies, including cattle complementary feed search, water for drinking and improving genetic performance for their cattle. The perceived changes and sociodemographic characteristics including religion, sociocultural group, etc. negatively influenced the adoption of some adaptation strategies such as forage reserves and changing transhumance area and routes. In the other hand, the perceived changes and socioeconomic characteristics including age, education, religion, etc. positively influenced the adoption of food stockpiling, of prayers and offering to deities, of changing the transhumance period, etc. The perception of the climate change effects and the sociodemographic characteristics of the transhumant herders affected the adoption of adaptation strategies in many ways. Policy makers could deliver climate information for strengthening the resilience of transhumant herders.
Enhanced production of thermostable catalase for efficient gluconic acid biocatalysis
Authors: Huang Jiang , Wang Jun , He Jinling , Wu Yupeng , Chen Lizhi , Zhou Shuangzi , Bian Yeyu , Li Yangyuan
Published: December 2024

Introduction

The demand for gluconic acid (GA) has risen recently, driven by its extensive applications in the food, healthcare, and construction industries. The biocatalysis of gluconic acid, facilitated by glucose oxidase and catalase, hinges on enzyme stability, significantly influencing catalytic efficiency. Nonetheless, catalase requires enhancements in thermal stability and activity to meet the requirements of practical applications.

Methods

We evaluated ten catalases expressed in Aspergillus niger, ultimately selecting the catalase from the thermophilic fungus Thermoascus aurantiacus, labeled as TaCat, for its superior thermal stability and operational performance. We further characterized the enzymatic properties of the recombinant catalase, focusing on its thermostability. Simultaneously, we used AlphaFold2 for structural predictions and conducted in-depth analyses via accelerated molecular dynamics simulations.

Results and discussion

We successfully obtained a strain with the highest catalase activity by optimizing signal peptides and overexpressing the crucial heme synthesis enzyme. Enzyme production reached an impressive 321,779.5 U/mL in a 50-L fermenter. Our application studies confirmed the considerable advantages of TaCat in terms of GA production. In conclusion, TaCat, distinguished by its remarkable thermal stability and high activity, holds substantial potential for GA production.
Characterization of risk factors for early ambulation in paraplegic dogs with absent pain perception undergoing decompressive surgery for thoracolumbar intervertebral disk extrusions
Authors: Gomes Sérgio A. , Alvites Rui Damásio , Lopes Bruna , Coelho André , Targett Mike , Ribeiro Jorge , Gouveia Débora , Martins Ângela , Varejão Artur S. P. , Maurício Ana Colette , Luís Ana Lúcia
Published: December 2024

Background

Current literature warrants surgical decompression in paraplegic dogs with absent pain perception (APP), but the rate of ambulatory dogs with APP following thoracolumbar (TL) IVDE surgery in a clinical setting remains unknown. Furthermore, the outcome of paraplegic APP French Bulldogs (FBs) is anecdotally considered poor. The aims of this study were threefold within a large population of TL-IVDE paraplegic dogs with APP undergoing decompressive surgery: (1) to characterize early spontaneous pelvic limb movement and ambulation following surgery; (2) to identify risk factors for the recovery of ambulation; and (3) to compare the outcome of FBs and Dachshunds presenting with APP.

Methods

A single-center, retrospective case series of dogs with paraplegia and APP diagnosed with TL-IVDE based on CT or MRI, all undergoing surgical decompression (hemilaminectomy ± durotomy). Two main groups were defined: ambulatory and non-ambulatory. These were further characterized depending on the presence of pain perception and spontaneous movement. The outcome was obtained at 4–8 weeks postoperatively. Statistical analysis was performed comparing ambulatory and non-ambulatory dogs and comparing rates of ambulation on FBs vs. Dachshunds.

Results

A total of 127 cases were included, with 77 out of 127 (60.6%) being ambulatory at recheck and 9 out of 127 (7.1%) being ambulatory despite APP. The remaining case distribution of non-ambulatory cases was: with APP (32 out of 127; 25.2%), with pain perception (5 out of 127; 3.9%), and with spontaneous movement (5 out of 127; 3.9%). Multivariate analysis revealed two negative factors for the recovery of ambulation: dogs undergoing hemilaminectomy alongside durotomy (p = 0.003) and dogs presenting with spinal shock (lower motor neuron signs with a lesion higher than the L3–L4 intervertebral disk) characterized by reduced/absent withdrawal reflex (p = 0.008). No difference was found between Dachshunds (n = 41, 73.2% ambulatory) and FBs (n = 33, 63.6% ambulatory) in terms of recovery of ambulation, with only 2 out of 33 (6.1%) FBs developing myelomalacia.

Conclusion

Early recovery of ambulation alone (60.6%) and ambulation alongside pain perception (53.5%) occurred in the majority of surgically managed TL-IVDE-affected dogs with APP. Negative prognostic factors for recovery of ambulation were durotomy performed alongside hemilaminectomy, and dogs presenting with spinal shock with reduced/absent withdrawal reflexes, the latter translating to a reduced/absent withdrawal reflexes with a lesion higher than L3–L4 intervertebral disk. Finally, no indications of a worse prognosis for recovery of ambulation or a higher rate of development of myelomalacia in FBs when compared to Dachshunds were found.
Handling Exponentially Growing Strategies in Spatial Cooperative Games: The Case of the European Union
Authors: Küçükmehmetoğlu Mehmet , Fahjan Yasin , Paköz Muhammed Ziya
Published: December 2024

This paper introduces a comprehensive cooperative game theory framework to measure the significance of location and neighborhood relations in conjunction with the magnitude of players/parties. The significances of these relations are measured over the EU geography. In this case, there are (i) the test of availability of a core solution that satisfies all associated parties/players; (ii) the measurement of players’/parties’ rational minimal and maximal return expectations from the grand coalition regarding their all individual and sub-group strategies and associated return rationalities; (iii) the determination of the critical players/parties in the grand coalition. The study’s main contributions are the provision of a methodology that identifies spatially/geographically critical players/parties and the design of an algorithm for handling exponentially growing strategies alongside increasing numbers of players/parties. In sum, a comprehensive cooperative game theory framework is introduced to measure the significance of location and neighborhood relations in conjunction with the magnitude of the players/parties. The case of the EU has revealed the union’s geographically critical countries, with Germany being found to be the most influential.
Does Having a Guardian with Cancer Contribute to Heightened Anxiety in Adolescents?
Authors: Forouzan Michaela , Quamruzzaman Amm , Sánchez-Jankowski Martin L.
Published: December 2024

When a guardian is diagnosed with cancer, the emotional and psychological toll they endure can have a profound impact on their children’s mental health. Understanding the factors that contribute to heightened anxiety in these children is crucial for identifying mental health disorders early. This cross-sectional study explored the relationship between having a guardian with cancer and elevated anxiety levels in adolescents, accounting for confounding variables such as sex, age, and socioeconomic status. Data were obtained from the 2022 National Health Interview Survey (NHIS) using the Sample Adult Interview (27,651 participants) and Sample Child Interview (7464 participants) datasets. The independent variable (guardian’s cancer diagnosis) was derived from the Sample Adult Interview, while the dependent variable (childhood anxiety) and confounders were derived from the Sample Child Interview. Using Stata 16.0, the datasets were merged based on household index variables, yielding a final sample of 4563 participants. Logistic regression analyses assessed the correlation between a guardian’s cancer diagnosis and anxiety levels in children. The results show that children with a guardian battling cancer are significantly more likely to develop anxiety (through the use of odds ratio), with the effect size varying based on factors such as sex, income, and environment. Girls, children from lower-income families, and those with a guardian experiencing depression were at particularly high risk. These findings highlight the strong link between familial health challenges and adolescent anxiety, emphasizing the need for early intervention and mental health support in families affected by cancer.
1997–2016, Twenty Years of Pollen Monitoring Activity in Rome Tor Vergata (Rome South-East): Trends Analysis
Authors: De Franco Denise , Di Menno di Bucchianico Alessandro , Travaglini Alessandro , Brighetti Maria Antonia
Published: December 2024

Global environmental change is rapidly altering the dynamics of terrestrial vegetation, with consequences for the functioning of the Earth system. Recent studies show that climate change is influencing the phenology and distribution of plants. Airborne pollen reflects the flowering period of the plant, which is influenced by meteorological variables such as temperature and rainfall. The analysis of pollen trends is a very useful tool for understanding the effects of climate change on vegetation. In fact, it is accepted that the onset and peak abundance of certain pollen types should be used as possible bioindicators of climate change. The aim of the work is to analyze the presence of various pollen in Rome—from their release from the anthers to their permanence in the atmosphere, the trends of phenological (start, length, and end of the pollen season) and production (pollen abundance and pollen peaks) pollen indicators, the trends of the meteorological variables mainly involved (temperature and precipitation), and any relationships between pollen and meteorological variables, also based on the variation in vegetation. In the period considered, the analysis of the pollen spectra shows an increasing trend in herbaceous taxa, probably attributed to a gradual abandonment of farming practices in the neighboring area, which in recent years has been the subject of intense new construction activity and to a progressive deterioration in the maintenance of green areas.
Patterns and Relationships of Pesticide Use in Agricultural Crops of Latin America: Review and Analysis of Statistical Data
Authors: Olguín-Hernández Licet , Carrillo-Rodríguez José Cruz , Mayek-Pérez Netzahualcóyotl , Aquino-Bolaños Teodulfo , Vera-Guzmán Araceli Minerva , Chávez-Servia José Luis
Published: December 2024

The excessive use of pesticides in agriculture causes problems related to public health; biodiversity; the contamination of water bodies, soil and air; and general environmental degradation, including interactions with climate change effects. The aim of this work was to describe the patterns of pesticide use in 17 Latin American countries and their associations with the total harvested area and the harvested areas of the five main crops on the basis of statistics reported by the Food and Agriculture Organization from 1990 to 2021. Principal component analysis (PCA) revealed four different patterns among countries according to the magnitude of pesticide application: Brazil (G1) > Argentina (G2) > Colombia and Mexico (G3) > Central and South America (G4). Together, more than 1.2 million tons of active ingredients is applied annually, carrying harmful effects and risks. In the joint analysis of both datasets (applied pesticides and harvested area), different correlations were observed between the amount of pesticides applied and the harvested area; for example, in G1 and G2, positive and significant correlations were detected between the applied amounts of total pesticides, herbicides, insecticides and bactericides and the total area harvested by the main crop, but in G3, there was a negative correlation with the five main crops.
A Review of Studies on the Effects of Anthropogenic Disturbances on Plant–Soil–Microorganism Interactions in Grassland Ecosystems: Based on Grazing and Tourism Perspectives
Authors: Chen Xingyan , Wang Xueling , Song Yuehua , Chi Yongkuan
Published: December 2024

As the most widely distributed and largest terrestrial ecosystem in the world, grasslands play an important role in supporting global livestock production and maintaining ecosystem services. In light of the accelerated global socio-economic development and sustained population growth, grassland ecosystems are increasingly subjected to anthropogenic disturbances. However, there is a paucity of research examining the impact of such disturbances on plant–soil–microorganism interactions in grassland systems, particularly from the perspectives of grazing and tourism. Accordingly, this study presents a comprehensive analysis of the impacts of anthropogenic disturbance on grassland ecosystems over the past two decades, employing a dual perspective of grazing and tourism and utilizing econometric analysis of the existing literature through software such as CiteSpace. The results of this study demonstrate the following: (1) The current research focus is primarily concentrated in the fields of ecology and environmental sciences, particularly on the topics of plant diversity, abundance, and diversity, as well as the intensity of grazing. These areas may represent key development direction of future research. (2) The impact of anthropogenic disturbances on grassland ecosystems is primarily associated with grazing activities. Moderate grazing disturbances can facilitate the healthy development of grassland ecosystems. However, the intermediate disturbance hypothesis (IDH) may not fully account for the effects of grazing intensity on grassland ecology. At present, there is still a paucity of systematic research to determine the ecological indicators of grassland under a dual-disturbance scenario. It is recommended that future research be carried out to investigate the compound effects of trampling by tourism activities on plant–soil–microorganism interactions in grassland ecosystems. (3) The mutual feedback mechanism may represent a potential mechanism by which anthropogenic disturbances affect the coupled relationship between the plant, soil, and microbial systems in grassland ecosystems. Furthermore, the interaction among these three systems has the potential to exert direct or indirect impacts on the structure and function of grassland ecosystems in the context of disturbances. The present study aims to provide an overview of the structure and function of grassland ecosystems under anthropogenic disturbances. The objective is to identify a balance between the rational use of grassland and ecological protection under anthropogenic disturbance and to provide scientific reference for the sustainable use of grassland worldwide.
Post-GWAS Prioritization of Genome–Phenome Association in Sorghum
Authors: Pal Debasmita , Schaper Kevin , Thompson Addie , Guo Jessica , Jaiswal Pankaj , Lisle Curtis , Cooper Laurel , LeBauer David , Thessen Anne E. , Ross Arun
Published: December 2024

Genome-wide association studies (GWAS) are widely used to infer the genetic basis of traits in organisms; however, selecting appropriate thresholds for analysis remains a significant challenge. In this study, we introduce the Sequential SNP Prioritization Algorithm (SSPA) to investigate the genetic underpinnings of two key phenotypes in Sorghum bicolor: maximum canopy height and maximum growth rate. Using a subset of the Sorghum Bioenergy Association Panel cultivated at the Maricopa Agricultural Center in Arizona, we performed GWAS with specific permissive-filtered thresholds to identify genetic markers associated with these traits, enabling the identification of a broader range of explanatory candidate genes. Building on this, our proposed method employed a feature engineering approach leveraging statistical correlation coefficients to unravel patterns between phenotypic similarity and genetic proximity across 274 accessions. This approach helps prioritize Single Nucleotide Polymorphisms (SNPs) that are likely to be associated with the studied phenotype. Additionally, we conducted a complementary analysis to evaluate the impact of SSPA by including all variants (SNPs) as inputs, without applying GWAS. Empirical evidence, including ontology-based gene function, spatial and temporal expression, and similarity to known homologs demonstrates that SSPA effectively prioritizes SNPs and genes influencing the phenotype of interest, providing valuable insights for functional genetics research.
Salivary Immunoglobulin a Alterations in Health and Disease: A Bibliometric Analysis of Diagnostic Trends from 2009 to 2024
Authors: Jankowski Jakub , Nijakowski Kacper
Published: December 2024

Background/Objectives: Salivary immunoglobulin A (IgA) is a mediator of local immunity and host defence. Altered IgA levels may predispose to bacterial invasion of the mucosa in the gastrointestinal tract, including the oral cavity. Our study aimed to present the diagnostic trends related to salivary IgA in health and disease based on a bibliometric analysis of published papers between 2009 and 2024. Methods: By 14 September 2024, 1247 English original articles were found in the database Web of Science. We selected 838 records considering the diagnostic usefulness of IgA in human subjects. Based on bibliographic data, we created citation and keyword co-occurrence maps using VOSviewer 1.6.20. Results: Most articles belonged to the “Sport Sciences” category (n = 169), followed by the “Immunology” category (n = 93). The Brazilian researcher Alexandre Moreira from the University of Sao Paulo had the most published and most frequently cited papers. Most of the included articles came from the USA (n = 158), England (n = 105), Brazil (n = 95), and Japan (n = 95). The most cited article described research on IgA in response to SARS-CoV-2 infection (n = 690), but the subsequent two papers considered the role of salivary IgA in the dysbiosis of the intestinal microbiota in inflammatory bowel diseases (n = 272) and the formation of systemic immune responses from the gastrointestinal tract (n = 245). Conclusions: Salivary IgA is a widely evaluated diagnostic marker in both patients and healthy individuals. Numerous reports have identified its changes as a result of physical exertion in various groups of athletes, during infections (including SARS-CoV-2) and in the course of local diseases (e.g., periodontal disease) or systemic diseases (e.g., inflammatory bowel disease).
Duration of Antibiotic Treatment for Foot Osteomyelitis in People with Diabetes
Authors: Gramberg Meryl Cinzía Tila Tamara , Torensma Bart , van Asten Suzanne , Sieswerda Elske , Sabelis Louise Willy Elizabeth , den Heijer Martin , de Vries Ralph , de Groot Vincent , Peters Edgar Josephus Gerardus
Published: December 2024

Background: The optimal antimicrobial treatment duration for diabetes-related foot osteomyelitis (DFO) currently needs to be determined. We systematically reviewed the effects of short and long treatment durations on outcomes of DFO. Methods: We performed a systematic review searching Cochrane, CENTRAL, MEDLINE, Embase, and CINAHL Plus from inception up to 19 January 2024. Two independent reviewers screened the titles and abstracts of the studies. Studies comparing short (<6 weeks) and long (>6 weeks) treatment durations for DFO were included. The primary outcome was amputation; the secondary outcomes were remission, mortality, costs, quality of life, and adverse events. Risk of bias and GRADE were assessed. Results: We identified 2708 references, of which 2173 remained after removing duplicates. Two studies were included. Differences in methodology precluded a meta-analysis. The primary outcome, major amputation, was reported in one study, with a rate of 10% in both the intervention and comparison groups (p = 1.00), regardless of treatment duration. For the secondary outcome, remission rates, the first study reported 60% in the intervention group versus 70% in the comparison group (p = 0.50). In the second study, remission rates were 84% in the intervention group versus 78% in the comparison group (p = 0.55). Data for the outcomes mortality, costs, and quality of life were not available. Short treatment duration may lead to fewer adverse events. The risk of bias was assessed as low to moderate, and the level of evidence ranged from very low to moderate. Conclusions: Our findings suggest that for DFO, there is no difference between a shorter and more prolonged duration of antimicrobial treatment regarding amputation and remission, with potentially fewer adverse events with shorter treatment durations. However, the uncertainty stems from limited, heterogeneous studies and generally low-quality evidence marred by moderate biases, imprecision, and indirectness. More high-quality studies are needed to substantiate these findings.
Automated Deep Learning Model for Sperm Head Segmentation, Pose Correction, and Classification
Authors: Guo Yunbo , Li Junbo , Hong Kaicheng , Wang Bilin , Zhu Wenliang , Li Yuefeng , Lv Tiantian , Wang Lirong
Published: December 2024

Male infertility remains a significant global health concern, with abnormal sperm head morphology recognized as a key factor impacting fertility. Traditional analysis of sperm morphology through manual microscopy is labor-intensive and susceptible to variability among observers. In this study, we introduce a deep learning framework designed to automate sperm head classification, integrating EdgeSAM for precise segmentation with a Sperm Head Pose Correction Network to standardize orientation and position. The classification network employs flip feature fusion and deformable convolutions to capture symmetrical characteristics, which enhances classification accuracy across morphological variations. Our model achieves a test accuracy of 97.5% on the HuSHem and Chenwy datasets, outperforming existing methods and demonstrating greater robustness to rotational and translational transformations. This approach offers a streamlined, automated solution for sperm morphology analysis, providing a reliable tool to support clinical fertility diagnostics and research applications.
Comparative Analysis of Analogue and Digital Methods for Magnetic Flux Estimation in the Core of a Medium-Voltage Voltage Transformer Operating Under Ferroresonance Conditions
Authors: Suchorolski Piotr , Smolarczyk Adam , Łukaszewski Piotr , Łapczyński Sebastian , Szulborski Michał , Owsiński Maciej , Łukaszewski Artur , Kolimas Łukasz , Nogal Łukasz
Published: December 2024

The main objective of the research described in the article was to determine the following: Is it possible to estimate the magnetic flux linkage in the core of a voltage transformer using analogue or digital methods? Will it be possible to estimate it approximately both in the normal state and in the state of deep core saturation (ferroresonant state)? The research aimed to identify the advantages and disadvantages of both proposed estimation methods. As part of the research described in this paper, a simulation model was developed and executed in the MATLAB/Simulink environment to generate a series of secondary voltage and flux waveforms in voltage transformers. The secondary voltage and flux waveforms were modelled under ground fault conditions, initiating ferroresonance oscillations in the medium-voltage network. To determine the associated flux from the simulated secondary voltages of the voltage transformers, an analogue integration circuit, a voltage analogue input circuit and a numerical integration algorithm with offset elimination were developed and implemented in an STM32 microcontroller. The obtained reference flux waveforms were used to verify the accuracy of the estimation of flux waveforms obtained using the analogue and digital methods. As a result, it was determined that both methods allow for a relatively accurate estimation of the periodic component of the magnetic flux. It also presented how both methods respond to the presence of slowly changing (aperiodic) components. Possible applications were proposed in order to create an innovative criterion for detecting ferroresonance oscillations.
An Experimental Study on Repairing of Reinforced Concrete Beams Having Damaged Longitudinal Bars
Authors: Ben Saoud Mouad , Orafi Mohamed , Gundogay Abdullah , Yaman Sila , Eren Volkan , Tekeli Kabas Hamide
Published: December 2024

The objective of this study was to systematically evaluate the effects of different repair methods to determine optimal strategies for enhancing the load-carrying capacity of damaged reinforced concrete beams. During construction or rehabilitation, some openings may be created in structural members for various reasons, either intentionally or accidentally. While creating these gaps, damage may occur to the lower reinforcement of the beam. Within the scope of this paper, the effects of these openings were studied, and the different techniques to be used in the repair of damaged reinforced concrete beams were investigated. This study discusses an experimental analysis of ten beams under bending loads. An opening gap was formed at the lower mid-span of all beams except the reference beam, with the main reinforcement in these openings being cut. The damaged beams were then repaired with various techniques, including fiber-reinforced polymer (FRP) sheets and different reinforcement bars. The experiments of all beams were carried out by applying the four-point bending test model. The results showed that all repaired beams had significant enhancements in behavior and load, stiffness, ductility, and energy consumption capacities compared to the damaged beam.
Blanching Temperature and Time Effects on the Chemical Composition of Sargassum horneri and Its Rumen Fermentation Characteristics and Greenhouse Gas Emissions
Authors: Wardani Arrynda Rachma Dyasti , Seo Myeong-Ji , Kim Hyun-Chul , Hwang Il-Ki , Kim Shin-Kwon , Baeg Chang-Hyun , Kim Ji-Yoon , Kim Sam-Churl
Published: December 2024

This study aimed to improve the nutritional value of Sargassum horneri (SH) and evaluate its effect on rumen fermentation characteristics and greenhouse gas emissions. The first trial estimated the effects of blanching temperatures (21, 60, 70, and 80 °C) and durations (1, 2, and 3 min) on the chemical and mineral content of SH. The second trial evaluated the effects of SH supplementation levels (0, 1, 3, and 5%) and blanching temperatures (21 and 70 °C) on in vitro rumen fermentation characteristics and greenhouse gas emissions. As the blanching temperature and time increased, the dry matter and crude ash content decreased (p < 0.05), and the ether extract, neutral detergent fiber, and acid detergent fiber content increased (p < 0.05). There were significant reductions in Na, K, and As (p < 0.01) when the blanching temperatures increased. As the SH supplementation levels increased, in vitro digestibility of both dry and organic matter decreased (p < 0.001) and the methane production reduced (p < 0.001). Therefore, this study shows that blanching SH at 70 °C for 3 min optimized its nutritional value and improved its potential as a feed source that has methane mitigation characteristics at 3 to 5% supplementation levels.
The Real-Time Observation of Electric Vehicle Operating Points Using an Extended Kalman Filter
Authors: Djellouli Younes , Ardjoun Sid Ahmed El Mehdi , Zerdali Emrah , Denai Mouloud , Chafouk Houcine
Published: December 2024

Electric Vehicles (EVs) are set to play a crucial role in the energy transition. Although EVs offer significant environmental benefits, their technology still faces major challenges related to performance optimization, energy efficiency improvement, and cost reduction. A key point to address these challenges is the accurate identification of the speed/torque operating points of the drive systems. However, this identification is generally achieved using mechanical sensors, which are fragile, bulky, and expensive. This paper aims to develop, implement, and validate a speed/torque observer in real time based on the Extended Kalman Filter (EKF) approach for an EV equipped with an Open-End Winding Induction Motor with Dual Inverter (OEWIM-DI). The implementation of the EKF is based on the state modeling of the OEWIM-DI, enabling the observation of the torque and speed using voltage and current measurements. The validation of this approach is conducted experimentally on the FPGA and DS1104 boards. The results show that this approach offers excellent performance in terms of accuracy, stability, and real-time response speed. These results suggest that the proposed method could significantly contribute to the advancement of EV technology by providing a more robust and cost-effective alternative to traditional mechanical sensors while improving the overall efficiency and performance of EV drive systems.