bluuebunny/crossref_metadata_2025 huggingface dataset:
Lesiones pulmonares quísticas en el neonato: no todo es enfermedad congénita pulmonar. Reporte de caso
Authors: Martínez-Rodríguez Laura Paola , Moreno-Gómez Luz Ángela , Carrillo-Bayona Jorge Alberto , Rueda-Badillo Edward Yesid
Published: August 2022

Los quistes pulmonares unilaterales son un hallazgo inusual en neonatos, desde el punto de vista del radiólogo es difícil distinguir los quistes pulmonares congénitos de los adquiridos. El diagnóstico definitivo es histológico. Se presenta un recién nacido prematuro de 28 semanas con síndrome de dificultad respiratoria en relación con múltiples quistes pulmonares. Las radiografías iniciales del tórax mostraron un quiste pulmonar solitario izquierdo. Requirió de forma escalonada asistencia respiratoria, junto con rayos X seriados donde se observa la presencia de neumotórax y el aumento del tamaño de la lesión quística inicial con presencia de nuevos quistes. La tomografía computarizada confirmó la presencia del quiste solitario del lóbulo inferior izquierdo y varias colecciones de gas intersticial con puntos centrales de tejidos blandos con relación a enfisema pulmonar intersticial y se planteó como diagnóstico diferencial malformación adenomatoidea quística. La lesión fue extirpada quirúrgicamente en vista del deterioro clínico. La histología confirmo la presencia de enfisema pulmonar intersticial persistente. MÉD.UIS. 2018;31(1):65-70.
Colgajo de Atasoy: revisión de una técnica clásica para reconstrucción digital
Authors: González-Cely Adriana Marcela , Miranda-Díaz Audrey Jose , Alviar-Rueda Juan Darío , Forero Pedro Luis
Published: August 2022

Aproximadamente el 50% de las lesiones de mano atendidas en urgencias corresponden a la punta de los dedos, dentro de estas el manejo de las amputaciones requiere una cobertura adecuada, aseguramiento de la sensibilidad táctil, mantención de la longitud y provisión de un lecho de crecimiento y almohadilla para la uña y pulpejo respectivamente; así, con resultados estéticos y funcionales que permitan una incorporación laboral temprana. El presente artículo realiza la descripción de la técnica colgajo de Atasoy o de V en Y, una herramienta que logra las metas reconstructivas en amputaciones dorsales oblicuas o transversas. Lo anterior basados en la literatura, disecciones en cadáveres y casos clínicos. MÉD.UIS. 2018;31(1):57-63.
Impactos dos estresses hídrico e salino em mudas de Erythrina velutina Wild
Authors: Aderaldo Francisco Ícaro Carvalho , Ferreira Gabriela de Sousa , Sousa Janacinta Nogueira de , Brito Paulo Ovídio Batista de , Gondim Franklin Aragão
Published: August 2022

O presente artigo analisou o crescimento de mudas de mulungu (Erythrina velutina Wild), submetidas a estresse salino e associado à suspensão de irrigação. Esse experimento foi realizado em vasos de 5 litros, irrigados a 50% da capacidade de campo. O delineamento experimental foi o inteiramente casualizado composto por seis tratamentos: T1- Irrigação com água destilada (Testemunho); T2 - Irrigação com solução de NaCl a 50 mM; T3 – Irrigação com solução de NaCl a 100 mM; T4 - Irrigação com água destilada com irrigação suspensa aos 15 dias após a semeadura (DAS); T5 - Irrigação com solução de NaCl a 50 mM com irrigação suspensa aos 15 DAS; T6 - Irrigação com solução de NaCl a 100 mM com irrigação suspensa aos 15 DAS. O tratamento testemunho acarretou menor quantidade de efeitos deletérios para as mudas sendo, portanto, o mais indicado para o cultivo de mulungu. A salinidade ocasionou reduções nas variáveis de crescimento analisadas, tais como, matéria fresca total, da raiz e da parte aérea, matéria seca total, da raiz e da parte aérea, altura, diâmetro dos caules e número de folhas. Esses efeitos foram intensificados nas mudas submetidas a 100 mM de NaCl e suspensão de irrigação.
Dinâmica de uso e ocupação da terra no município de Novo Repartimento, Pará
Authors: Souza Manuela Braga de , Mesquita Ana Luísa da Costa , Silva Júnior Orleno Marques da , Cardoso Pedro Monteiro , Ruivo Maria de Lourdes Pinheiro , Paiva Paula Fernanda Pinheiro Ribeiro
Published: August 2022

O município de Novo Repartimento, Pará, passou por um processo desordenado de expansão urbana, devido a intensificação das atividades agropecuárias, que desencadeou a ocupação de áreas ambientalmente frágeis. Essa ocupação acentuou impactos negativos causados pelo desmatamento alterando as paisagens. Com isso, o objetivo do estudo foi analisar e avaliar a evolução de uso e ocupação da terra com ênfase nas áreas desmatadas. Os procedimentos consistiram na elaboração e cruzamento de mapas temáticos, a fim de estabelecer classes hierárquicas da variável desmatamento; análise multitemporal da evolução das classes de uso e ocupação entre dois períodos distintos: 2000 e 2019, com auxílio das geotecnologias. Utilizou-se dados cartográficos disponíveis no banco de dados do Instituto Brasileiro de Geografia e Estatística, do Projeto MapBiomas e do Google Earth Engine. Os resultados demonstraram a importância do uso das geotecnologias para o planejamento sustentável da ocupação na terra em que, observa-se que o desmatamento no município é crescente, sendo que dentro das principais variáveis e correlações, destaca-se o setor agropecuário e madeireiro.
Horta escolar implementada em Associação de atendimento a pessoas com deficiências
Authors: Bennedetti Luiza Vigne , Guimarães Angela , Teixeira Bruna Raquel Rodrigues , Klein Maico Ismael , Santos Angélica Gomes Florczak dos , Richter Marc François
Published: August 2022

O objetivo do presente trabalho foi avaliar os benefícios observados após a implementação de uma horta escolar em Associação que atende pessoas com deficiências no município de Paiçandu/PR. Trata-se de um estudo transversal descritivo que analisou as percepções de indivíduos envolvidos com o projeto da horta, as quais foram coletadas de professores da instituição, pais de alunos matriculados e funcionários da empresa que atuaram no projeto. Entre os resultados, foi possível observar distintos benefícios da horta, especialmente concernentes a aspectos sociais, pedagógicos, alimentares e ambientais. Observou-se relatos positivos de pais e professores, particularmente relacionados às áreas social e pedagógica. Destaca-se também o papel de transformação socioambiental exercido pela empresa envolvida no projeto.
Causas de perdas pós-colheita em cultivares de tomates comercializados na Ceasa, Ceará
Authors: Teixeira Isla Simplicio , Rufino Maria do Socorro Moura , Pinto Ciro de Miranda , Almeida Antônio Odálio Girão de
Published: August 2022

A pesquisa consistiu em identificar as perdas pós-colheita das cultivares de tomate ‘Longa Vida’ e ‘Cajá’ comercializadas na Ceasa-CE, identificando as principais dificuldades de sua comercialização. As ações foram avaliadas em duas etapas: Entrevistas direcionadas a 50 permissionários que comercializam o tomate; Avaliação das principais perdas do tomate em bancadas e/ou boxes. Os frutos antes da comercialização, foram submetidos às análises de amostragem contendo 899,4 kg na amostra “Qualidade Inicial”, para determinar os principais danos e 191,8 kg na amostra “Descarte”, para determinar a perda real do produto. Posteriormente, foi calculado a porcentagem e a média dos frutos em diferentes estádios de maturação (verdes, maturidade horticultural e completamente maduros), com injúrias mecânica, fisiológica, doenças fitopatológicas, ataque de pragas e ausência de danos. A cultivar Longa Vida é a mais comercializada na Ceasa (70%). O transporte é realizado em caminhões abertos (80%) e o armazenamento é em bancadas e/ou boxes (86%). Para a comercialização do tomate, são utilizadas embalagens plásticas (98%), sendo o manuseio incorreto o principal fator que dificulta a sua comercialização (32%). Na amostragem 'Qualidade Inicial', 68,99% das cultivares apresentaram-se maturidade horticultural (47 kg). 18,07% das injúrias mais frequentes tem-se a mecânica (12 kg) e 10,55% a danos fisiológicos (7 kg). Do total da amostragem “descarte”, cerca de 60, 58% (8 kg) dos frutos manifestavam-se maturidade horticultural e apresentaram 36,43% danos por injúria mecânica (48 kg) e 21,90% danos por ataque de pragas (2,9 kg). As perdas pós-colheita podem ser reduzidas com a implementação e uso de câmaras refrigeradas.
A prospective observational study on cystic stump closure during laparoscopic cholecystectomy: silk sutures (intra-corporeal ligation) or locking clips (Hem-o-Lok®)
Authors: YENİ Mustafa , KALAYCI Tolga
Published: August 2022

Objectives: The present study compares the clinicopathological features and outcomes of two cystic stump closure techniques (locking clips and intra-corporeal ligation with silk sutures) during laparoscopic cholecystectomy.
Methods: This study was conducted in a tertiary health centre as a prospective observational study between October 5, 2021, and February 15, 2022. For randomisation, double rows of silk sutures were used to close the cystic stump in patients on the odd-numbered day of the month (Group 1). In contrast, a single-row locking clip (Hem-o-Lok®) (Group 2) was used to close the cystic stump in patients on the even-numbered day of the month. The study was terminated when at least 50 patients were reached in both groups. The clinicopathological differences and cholecystectomy outcomes were compared between the groups.
Results: This observational study included 114 patients. Of the study cohort, 87 (76.3%) were female, and the mean age was 46.54 ± 14.74 years. There were 64 (56.1%) patients in Group 1 and 50 (43.9%) patients in Group 2. Preoperative ERCP requirement was higher in Group 1 (15.6% vs 4%, p = 0.045), while the mean operation room time was longer in Group 2 (p = 0.015). Morbidity rates were similar in the two groups (3.1% vs 2%) (p > 0.05).
Conclusions: Cystic stump closure is the essential step of laparoscopic cholecystectomy. According to the present study’s results, silk sutures (intra-corporeal ligation) and locking clips (Hem-o-Lok®) are materials that can be used safely to close a cystic stump.
The German Enforceable Notarial Act in Cross-Border Cases
Authors: Bormann Jens
Published: August 2022

The subject of this article is to explain and discuss the legal framework for German enforceable notarial acts in cross-border traffic. The first part will illustrate the prerequisites for the enforcement of a notarial act in Germany, especially in circumstances when the creditor or debtor lives abroad. The second section is dedicated to the enforcement of German enforceable notarial acts abroad. After presenting the principles of international law, the main legislative acts regarding enforcement in the European and international framework will be analysed in detail.
Justification of Taxation with Flat-Rate Income Tax on Real Estate Renting for Tourist Purposes in the Republic of Croatia
Authors: Pribisalić Luka
Published: August 2022

Income from renting apartments, rooms, and beds to travelers and tourists and organizing camps in the Republic of Croatia is taxed at a flat rate. The question is whether the taxation of this type of activity is justified if it generates more income than from renting/leasing real estate under a lease or rental agreement when these activities are taxed with a higher amount of tax from renting real estate for tourism purposes. Therefore, it is necessary to analyze why the flat-rate taxation regime for the activity of renting apartments, rooms, and beds to travelers and tourists and the organization of camps is prescribed in the Republic of Croatia and whether it is justified.
If at First You do Not Succeed: An Overview of Remedies Available in the United States Courts of Appeals
Authors: Heller Thomas Allan
Published: August 2022

In the United States federal court system, from a procedural standpoint, it has been the historic policy that appeals to the Courts of Appeal lie only from final decisions by the district courts.  This policy, dubbed the final judgment rule, is designed to prevent a piecemeal approach to appellate practice, and to enhance efficiency and fairness.  Applied overly strictly, the rule can often lead to unfair results, and even irreparable harm. This article catalogues the primary exceptions to the final judgment rule, and discusses those instances when interlocutory appeals may be taken short of district court rulings disposing of all issues as to all parties, that is, final judgments.
Legal Aspect of Organ Donation after Death across Europe in Human Rights Context
Authors: Ilona Kiel-Puślecka  , Mateusz Puślecki  , Marek Dąbrowski  , Bartłomiej Janyga  , Bartłomiej Perek  , Agnieszka Zawiejska
Published: August 2022

Clinical transplantation has proven to be lifesaving methods since last century. Organ transplants is still subject to ethical evaluation through the prism of basic standards of medical ethics and social phenomena that are not morally neutral. Transplant medicine has a clear social character. It is not only a relationship between a doctor and a specific patient. Authors analyzed existing postmortal donation models in European countries and the most important documents in the European legislation in securing universal rights to freedom and human dignity in transplantation area and identified 15 universal documents valid in Council of Europe and the European Union. Universal legal documents of European law protect human donor right to self-determination and integrity. Postmortal donation in transplantation performed in accordance with the applicable legislation and in the utilitarian dimension does not violate human dignity and the natural right of a person to decide about themselves.
Communicating about Healthcare Reform as Part of Good Governance During the Covid-19 Pandemic. Experiences from Finland and Poland
Authors: Natalia Kohtamäki  , Sebastian Sikorski
Published: August 2022

The right to information, understood as a citizen’s right of access to data used in the process of making and enforcing laws, is a natural component of democratic systems. In the rich societies of Western Europe, which for years have been supporting civic education, society consciously uses the right to information. It is the interaction of citizens with decision-makers among others in the process of public information flow that changes ordinary public governance into good governance. The authors refer to the contextual understanding of good governance within the framework of multi-level interactions at the level of central and local government. Good governance should be understood as the achievement of ‘public value’ – i.e., the shared needs of many citizens but within the context of innovation and the economic development of societies. Informing means strengthening trust in public authorities, which in democratic states should have nothing to hide (even in a crisis).
Cross-Border Provisional and Protective Measures for Preserving of Physician's Funds Subject to a Liability Claim in the EU
Authors: Urška Kupec
Published: August 2022

Patients frequently opt for medical treatment outside the public health system. This means that the patient enters into a contract with an individual physician, which commits the physician to perform a medical procedure for the patient, and the patient undertakes to pay for this procedure. If the physician does not act with due diligence or does not achieve an outcome that they have explicitly committed to reach, a situation may arise where the patient has a monetary claim against the physician. If the physician does not repay the claim voluntarily, the patient has certain options available to ensure that their claim is forcibly repaid. In this paper, we deal with the position of the +patient as a creditor who does not yet have an enforceable title in relation to the physician and the debtor, and with a patient who already has such an enforceable title. The field of study is limited to the situation when Slovenian law applies for decision on the responsibility of a physician and patient wants to claim the funds that the physician has in bank accounts in the European Union.
Digital Transformation as One of the Instruments for Overcoming the Public Health Crisis: The Role and Use of Ehealth Solutions during the Covid-19 Pandemic in Slovenia
Authors: Dalibor Stanimirović  , Matjaž Drev  , Živa Rant
Published: August 2022

The comprehensive digital transformation of the Slovenian healthcare system and the use of national eHealth solutions would enable the citizens and healthcare professionals to access data and information relevant to highquality healthcare services, and healthcare managers to take evidence-based decisions and measures. This paper analyses the role and use of selected eHealth solutions during the Covid-19 pandemic in Slovenia. The pandemic has led to significant growth in interest in digital transformation processes and the use of eHealth solutions. It also breaks down the normative framework of eHealth and the compliance of eHealth solutions with the fundamental principles of information security and the General Data Protection Regulation. The study proceeded from a review of the literature, followed by discussions with experts engaged in managing eHealth solutions and an examination of current statistical data on the use of these solutions from administrator modules. The results of the study show that the Covid-19 pandemic could constitute a turning point in our understanding of digital transformation, which is becoming not only a key public health management instrument, but also an indispensable driver to further develop the healthcare system, and empower patients at times of national and international public health crises.
Cyber Security in the Age of Covid-19: A Timeline and Analysis of Cyber-Crime and Cyber-Attacks during the Pandemic
Authors: Niteesh Kumar Upadhyay  , Mahak Rathee
Published: August 2022

The entire world has been impacted by Covid-19, which has forced the nations to undergo lockdowns, which have opened the new horizons of virtual learning and work from home culture. Though virtual learning and online meetings were available prior to the lockdowns, use of these platforms have intensified. There is a lack of awareness among many in the public concerning the perils of using these platforms which makes them susceptible to cyber-crime attacks phishing, sexual or verbal abuse, eve teasing etc. Lockdowns have provided cyber criminals with new criminal opportunities and evidence shows that there has been a rampant increase in the number of cyber-crimes during this period. Public lack of awareness has led to innocent people falling prey in the hands of attackers. Since the pandemic and ensuing lockdowns came as a surprise, public and private authorities lacked the opportunity to make appropriate arrangements for training people and making these platforms secure. There is no question that if these platforms are made secure, then they will prove to be as an asset to the society but much work must be done to achieve the goal of complete cyber security.
Economic Impact of Climate Change on Food Crop Production using Ricardina Approach: A Case of Kellem Wollega Zone, Ethiopia
Authors: Terefe Melkamu Kena
Published: August 2022

Background: Historical records and increased scientific consensus provide strong evidence that the global climate is changing. Future climate change would lead to an increase in climate variability and the frequency and intensity of extreme events. Agriculture that is dependent on climate conditions is inherently sensitive to climate changes. Today’s world agriculture has gone through challenges from population growth and has adapted to changing economic conditions, technology and resource availabilities. But uncertainty remains concerning the ability of agricultural systems to adapt to climate change. Thus, climate change adaptation of the essence for a resilient food crop production system requires economic transformation by arrangement of institutional and technology. Methods: This study uses the Ricardian model to examine the economic impact of climate change on agriculture in Kellem Wollega Zone, Ethiopia. The net farm revenue is regressed against climate variable (temperature and precipitation), soil and socio-economic variables to help determine the factors that influence variability in net farm revenues. The study was based on the data from a survey of 400 smallholder farming households interviewed across the zone. Result: The Ricardian model analysis shows the coefficients of summer, autumn and winter temperature are positive whereas the coefficient of spring temperature is negative. Regarding the precipitation, the coefficients of summer and spring precipitation are positive while coefficients of autumn and winter precipitation are negative. The marginal impact analysis results show an increase in summer and spring temperatures has mostly negative effects on net farm revenues implying that further temperature increases would be harmful to agricultural activities while increases in autumn temperatures increase net farm revenues in the study area. The summer and spring precipitation would increase the net farm revenue but the autumn precipitation reduces the farm revenue. The elasticity results show that net farm revenues are highly sensitive to changes in climate and the elasticity is relatively high for both summer temperature and precipitation. The impacts of climate change under the three special Reports on Emission Scenarios, (Canadian General Circulation Model, Hadley Centre for Climate Prediction and Research and Parallel Climate Model) predicted that by 2100 net farm revenues would decrease across all farms per hectare by US$ 942.83, US$ 1048.16 and US$ 1024.32 respectively. The finding suggests there is a great need for the concerned bodies to provide up-to-date information about climate change and rainfall patterns in the forthcoming season so that the farmers make informed decisions and develop adaptation strategies.
Response of Groundnut (Arachis hypogaea) under Different Phosphorus Management Options in Central Dry Zone of Karnataka
Authors: Naik A.H. Kumar , Naik T. Basavaraj , Kumara O. , Umesha S. , Madhu G.
Published: August 2022

Background: Phosphorus is considered as the second most important plant nutrient because of its significant role in various biochemical activities such as photosynthesis, respiration, root growth, energy storage, cell division etc. Phosphorus is required in greater amounts for pulse crops because of high demand in energy transfer molecules used in nitrogen fixation. Application of proper dose of phosphorus fertilizer is very crucial in deciding the productivity of the groundnut. The bioavailability of the phosphorus fertilizers mainly depends on the soil properties viz., pH, organic carbon, amount of Fe and Al oxides, CaCO3 and microbial load in the soil. In this context, the present study was designed to know the growth and yield parameter of groundnut by using appropriate combination of microbial culture with different levels of phosphorus at central dry zone of Karnataka. Methods: Field experiments were carried out for three consecutive years during 2018, 2019 and 2020 at Zonal Agricultural and Horticultural Research Station, Babbur farm, Hiriyur, Karnataka, India to study and understand the efficiency of DGRC culture (microbial culture) with different levels of phosphorus on groundnut under kharif season. The experiment was laid out in randomized block design with three replication and ten treatments consisting of seed treatment with DGRC culture inoculants @ 20g/ kg groundnut seed (cv. TMV-2) with different doses of phosphorus (20 kg, 40 kg and 60 kg/ha). Result: Three years pooled results indicated that there was a significant response of pod yield (2457 kg/ha) and higher economics like gross return (Rs. 1,15,138/ ha), net return (Rs. 73,115/ha) and B:C ratio (2.76) were also profitable with the application of 40 kg/ha of P + DGRC culture as compared to other treatments. Furthermore, higher nutrient uptake viz., N (99.60 kg/ha), P2O5 (16.68 kg/ha) and K2O (58.86 kg/ha) were recorded in application of 40 kg/ha of P+DGRC culture.
L’engagement Sociétal des Entreprises et Politique Commerciale: Cas des Entreprises Marocaines Labellisées RSE
Authors: Jihane Dinia
Published: August 2022

La thématique de RSE occupe l’intérêt des milieux académiques depuis plusieurs années et connait un grand essor dans la pratique des entreprises. Toutefois, les recherches antérieures ont porté plus sur les fondements théoriques, l’évolution des acceptions, l’analyse des implications sociétales et organisationnelles, voire, les effets de la RSE sur la performance de l’entreprise. Alors, peu d’intérêt a été accordé aux motivations des entreprises à l’engagement sociétal, en l’occurrence, les motivations commerciales, d’où la perspective du présent travail. Il s’agit de contribuer à l’analyse de la RSE comme instrument de la politique commerciale des entreprises, à travers une étude empirique auprès des entreprises marocaines labellisées RSE de la CGEM.

Pour ce faire, nous optons pour un positionnement « positiviste aménagé » avec une démarche « hypothético-déductive », et une méthodologie d’étude quantitative. Sur le plan théorique, nous essayons d'établir un cadre d’analyse de l’approche instrumentale des motivations à l’engagement RSE. En s’appuyant sur ce développement, nous construisons nos hypothèses à vérifier qui sont testées selon l’approche PLS et traitées en utilisant le langage R. Au niveau des résultats, l’étude empirique permet de conclure que l’engagement RSE contribue bien à atteindre les objectifs de la politique commerciale de l’entreprise.

The CSR’s thematic has been of interest to the academic community for several years and is experiencing a tremendous appeal in business practice. However, previous research focused more on the theoretical foundations, the evolution of acceptances, the analysis of the societal and organizational implications, and even the effects of CSR on the company’s performance. Consequently, little attention was paid to the reasons behind the adoption of the CSR approaches, in this case, the business motives, hence the perspective of this work. The aim is to contribute to the analysis of CSR as an instrument of the company’s commercial policy through an empirical survey study of CSR labeled Moroccan companies in the General Confederation of Moroccan Companies.

To do this, we opt for a "positivist" positioning with an hypothetico-deductive method and a quantitative study methodology. On the theoretical level, we are trying to establish a framework for the theoretical analysis of the instrumental approach to the motivations for CSR engagement. Based on this development, we are building our hypotheses to be verified which are tested according to the PLS approach and processed using the R language.

At the level of results, the empirical study concludes that societal commitment contributes well to achieving the objectives of the company's commercial policy.
The Communication and Dissemination of Chinese Characteristic Cuisine in the West: A Case Study on the Logistics Report for Lao Gan Ma from China to Australia
Authors: Yongshi QIAN
Published: August 2022

In today’s world, globalization has developed well, and it is in a potential and relatively stable state in both its depth and breadth. Building cultural confidence while strengthening cultural communication is an important issue facing every part of the world at present. Furthermore, the path of cultural communication is not limited to the cultural level itself but permeates all aspects of social life. Chinese cuisine has a long history and is well-known all over the world. This paper studies the spread of Chinese cuisine Lao Gan Ma to Australia, aiming to explore its cultural core through the analysis of the trade path of products.
Sağlık Kurumlarındaki Satınalma Çalışanlarının E-Eksiltme İhale Usulüne İlişkin Tutumlarının İncelenmesi
Authors: ŞAFAK Kerem , ULUDAĞ Ayhan
Published: August 2022

Çalışmanın amacı; Türkiye'de sağlık sektöründe yapılan ihalelerde seçilen yöntemlerin elektronik eksiltme açısından incelenmesi ve satınalma birimlerinde çalışanların elektronik eksiltme konusundaki görüşlerinin değerlendirilmesidir. Değerlendirme sonucunda e-eksiltme yöntemi seçiminin kamu kaynaklarının etkili kullanılmasına katkı sağlaması için çözüm önerileri sunmaktır. Araştırmanın evreni Sağlık Bakanlığına bağlı kuruluşlarda çalışan satınalma personeli oluşturmaktadır. Örneklem, yapılan hesaplama sonucunda 383 olarak bulunmuş ve 641 katılımcı ile çalışma gerçekleştirilmiştir. Araştırmaya katılanların %20,8’i Genel İdari Hizmetler Sınıfı (GİHS), %44,2’si Sağlık Hizmetleri Sınıfı (SHS) ve %35’i Yönetici sınıfında çalışmaktadır. Katılımcıların %49,1’i satınalma eğitimi alırken %50,9’u eğitim almamıştır. Araştırmaya katılanların %70,7’si satınalma biriminde isteyerek çalışırken, %29,3’ü ise isteği dışında bu birimde çalışmaktadır. Katılımcıların yaş değişkeni ile zamandan tasarruf sağlama değişkeni arasında, satınalma biriminde isteyerek çalışma ile verimlilik arasında, eğitim durumu ile verilen hizmetin kalite düzeyi arasında, eğitim alma durumu ile saydamlık arasında, memuriyet yılı ile olası çalışan hatalarının azalması arasında, eğitim durumu ile personel tasarrufu sağlama arasında ve unvan ile güvenirlilik düzeyi arasında anlamlı ilişki olduğu tespit edilmiştir (p < ,05). Çalışma sonucunda, satınalma biriminde çalışanların e-eksiltme usulünün ihalelerde saydamlık sağlayacağı, maliyetleri düşüreceği, verimliliği arttıracağı, yolsuzlukları azaltacağı, rekabeti arttıracağı, personel ve zaman tasarrufu sağlayacağını ifade etmelerine rağmen e-eksiltme konusunda yeteri kadar bilgi ve eğitim deneyimine sahip olmadıkları sonucuna ulaşılmıştır.
miR-490-3p Alleviates Cardiomyocyte Injury via Targeting FOXO1
Authors: Jiang Jin , Xu Jian , Tang Huifang
Published: August 2022

Background:

MicroRNA-490-3p (miR-490-3p) plays a role in the pathogeneses of a
variety of cardiovascular diseases. Bioinformatic analysis showed that miR-490-3p was downregulated
in the myocardial tissues of mice with myocardial infarction (MI). Nevertheless, the
functions and mechanisms of miR-490-3p in MI remain unclear.

Methods:

This study used an in-vitro model to investigate the role of miR-490-3p in MI. Human
cardiac myocytes (HCMs) were cultured in a hypoxic environment. 3-(4,5)-Dimethylthiahiazo (-zy1)-
3,5-di-phenytetrazoliumromide (MTT) assay and flow cytometry were used to detect cell
viability and apoptosis. The expression levels of forkhead box O1 (FOXO1) and miR-490-3p were
detected by quantitative real-time PCR and Western blot. The levels of interleukin-6 (IL-6), tumor
necrosis factor-α (TNF-α), interleukin-1β (IL-1β), lactate dehydrogenase (LDH), cardiac troponin I
(cTnI), and creatine kinase MB (CK-MB) were detected by enzyme-linked immunosorbent assay
(ELISA). The targeted relationship between miR-490-3p and FOXO1 3’UTR was determined by a
dual-luciferase reporter gene assay.

Results:

miR-490-3p was significantly down-regulated in hypoxia-induced HCM cells, while
FOXO1 was markedly up-regulated. miR-490-3p overexpression inhibited HCM cell inflammatory
responses and injury after hypoxia treatment. FOXO1 was validated to be a direct target of miR-
490-3p, and its overexpression weakened the effects of miR-490-3p on cell viability, apoptosis, as
well as inflammatory responses.

Conclusion:

miR-490-3p alleviates cardiomyocyte injury via targeting FOXO1 in MI.
Phosphorylation Regulation of a Histone-like HU Protein from
Deinococcus radiodurans
Authors: Hou Jinfeng , Dai Jingli , Chen Zijing , Wang Yudong , Cao Jiajia , Hu Jing , Ye Shumai , Hua Yuejin , Zhao Ye
Published: August 2022

Background:

Histone-like proteins are small molecular weight DNA-binding proteins
that are widely distributed in prokaryotes. These proteins have multiple functions in cellular
structures and processes, including the morphological stability of the nucleoid, DNA compactness,
DNA replication, and DNA repair. Deinococcus radiodurans, an extremophilic microorganism, has
extraordinary DNA repair capability and encodes an essential histone-like protein, DrHU.

Objective:

We aim to investigate the phosphorylation regulation role of a histone-like HU protein
from Deinococcus radiodurans.

Methods:

LC-MS/MS analysis was used to determine the phosphorylation site of endogenous
DrHU. The predicted structure of DrHU-DNA was obtained from homology modeling (Swissmodel)
using Staphylococcus aureus HU-DNA structure (PDB ID: 4QJU) as the starting model.
Two types of mutant proteins T37E and T37A were generated to explore their DNA binding
affinity. Complemented-knockout strategy was used to generate the ΔDrHU/pk-T37A and
ΔDrHU/pk-T37E strains for growth curves and phenotypical analyses.

Results and Discussion:

The phosphorylation site Thr37, which is present in most bacterial HU
proteins, is located at the putative protein-DNA interaction interface of DrHU. Compared to the
wild-type protein, one in which this threonine is replaced by glutamate to mimic a permanent state
of phosphorylation (T37E) showed enhanced double-stranded DNA binding but a weakened
protective effect against hydroxyl radical cleavage. Complementation of T37E in a DrHU-knockout
strain caused growth defects and sensitized the cells to UV radiation and oxidative stress.

Conclusions:

Phosphorylation modulates the DNA-binding capabilities of the histone-like HU
protein from D. radiodurans, which contributes to the environmental adaptation of this organism.
MicroRNA-137 Inhibits Esophageal Squamous Cell Carcinoma by Downregulating
DAAM1
Authors: Li Weina , Bai Xiangdong , Guo Ruyuan , Xing Xiaolong , Zhang Huanhu , Gao Xuezhen
Published: August 2022

Background:

A growing body of evidence demonstrates that miR-137 acts against
cancers; however, the biological function of miR-137 in esophageal squamous cell carcinoma
(ESCC) remains to be fully understood.

Objective:

The aim of this study is to explore the role of miR-137 in ESCC.

Method:

miR-137 expression was detected by reverse-transcription quantitative polymerase chain
reaction (RT-qPCR), and target protein expression was detected by western blot. Cell counting,
colony formation and flow cytometry were employed to determine the effects of miR-137 on the
growth of ESCC cells. Dual-luciferase reporter assay was performed to validate the binding of miR-
137 with a dishevelled-associated activator of morphogenesis 1 (DAAM1) 3’-UTR.

Results:

miR-137 was shown to be down-regulated in ESCC. miR-137 expression was inversely
correlated with the 5-year survival rate of ESCC patients. Up-regulated miR-137 attenuated ESCC
proliferation and promoted ESCC cell apoptosis. Meanwhile, to further reveal how miR-137
regulated the malignant behaviors of ESCC, the downstream mRNA binding targets of miR-137
were explored. miR-137 was demonstrated to bind DAAM1 3’-UTR and repressed the expression
of DAAM1. The expression of DAAM1 and miR-137 in ESCC was inversely correlated.
Additionally, the reintroduction of DAAM1 had the capacity to reverse the negative role of miR-
137 in ESCC cell growth.

Conclusions:

These findings have uncovered the new function of miR-137 in ESCC via negatively
regulating DAAM1, suggesting miR-137 as a potent therapeutic candidate for ESCC treatment.
Advances in the Discovery of Novel Inhaled PI3Kδ Inhibitors for the
Treatment of Asthma
Authors: Wei Jun , Gu Dongyan , Yuan Leer , Sheng Rong
Published: August 2022

Bronchial asthma is the most common chronic respiratory illness, the incidence
of which continues to increase annually. Currently, effective treatments for CS-resistant
asthma and severe asthma are still lacking, and new therapeutic regimens are urgently
required. PI3Kδ is a key enzyme in hematopoietic cells and represents a major target
for oncology and inflammatory disease (particularly respiratory disease, asthma and
COPD). In the case of respiratory disease, the ability to inhibit PI3Kδ in the lungs shows
a higher safety and therapeutic index relative to systemic inhibition. In recent years,
paradigm shifts have occurred in inhalation therapeutics for systemic and topical drug delivery
due to the favorable properties of lungs, including their large surface area and high
permeability. Pulmonary drug delivery possesses many advantages, including a non-invasive
route of administration, low metabolic activity, a controlled environment for systemic
absorption and the ability to avoid first bypassing metabolism. In this review, we
focus on the discovery and development of inhaled drugs targeting PI3Kδ for asthma by
focusing on their activity and selectivity, in addition to their potential in drug design
strategies using inhaled administration.
Application of Machine Learning Technology in the Prediction of ADME-
Related Pharmacokinetic Parameters
Authors: Wang Ying , Zhan Yonghua , Liu Changhu , Zhan Wenhua
Published: August 2022

Background::

As an important determinant in drug discovery, the accurate
analysis and acquisition of pharmacokinetic parameters are very important for the clinical
application of drugs. At present, the research and development of new drugs mainly
obtain their pharmacokinetic parameters through data analysis, physiological model construction
and other methods, but the results are often quite different from the actual situation,
needing more manpower and material resources.

Objective::

We mainly discuss the application of machine learning technology in the prediction
of pharmacokinetic parameters, which are mainly related to the quantitative study
of drug absorption, distribution, metabolism and excretion in the human body, such as
bioavailability, clearance, apparent volume of distribution and so on.

Method::

This paper first introduces the pharmacokinetic parameters, the relationship between
the quantitative structure-activity relationship model and machine learning, then
discusses the application of machine learning technology in different prediction models,
and finally discusses the limitations, prospects and future development of the machine
learning model in predicting pharmacokinetic parameters.

Results::

Unlike traditional pharmacokinetic analysis, machine learning technology can
use computers and algorithms to speed up the acquisition of pharmacokinetic parameters
to varying degrees. It provides a new idea to speed up and shorten the cycle of drug development,
and has been successfully applied in drug design and development.

Conclusion::

The use of machine learning technology has great potential in predicting
pharmacokinetic parameters. It also provides more choices and opportunities for the design
and development of clinical drugs in the future.
Selective Cytotoxicity and Changes in Protein Expression of T24 Bladder
Carcinoma Permanent Cell Line after Treatment with Hemocyanins
Authors: Dolashki Aleksandar , Antonova Olga , Velkova Lyudmila , Kaynarov Dimitar , Voelter Wolfgang , Dolashka Pavlina
Published: August 2022

Background:

Some molluscan hemocyanins (Hcs) have significant immunological and antitumor potential, enabling their application in oncology. The antitumor activity of Hcs from marine snails Rapana venosa (RvH), giant keyhole limpet Megathura crenulata (KLH) and garden snails Helix lucorum (HlH), as well as their different derivatives, were studied in vitro on a permanent T24 cell line of bladder cancer and normal urothelial cell line HL 10/29 compared to doxorubicin.

Methods:

The antiproliferative activity of the tested Hcs was determined using WST-1 assay and BrdU ELISA assay. Morphological changes in both urothelial cell lines were confirmed by fluorescence microscopy. The proteomic analysis of a cell line of bladder cancer before and after treatment with functional unit (FU) βc-HlH-h using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and mass spectrometry revealed differences in the expression of some proteins.

Results:

Studies prove that the T24 tumor cell line is dose- and time-dependent, sensitive to the action of the tested isoforms and the glycosylated FU of these hemocyanins. Selective inhibition of T24 cell growth was observed after incubation with structural subunits (βc-HlH, RvHI and RvHII) and FUs (βc-HlH-h and RvHII-e). Fluorescent microphotographs did not show apoptotic or necrotic alterations in the normal urothelial cell line HL 10/29. The FU βc-HlH-h demonstrated the highest antiproliferative effect (similarly to doxorubicin), in which predominantly apoptotic and less late apoptotic or necrotic changes in the tumor cells were observed. Several down- and up-regulated proteins identified by proteome analysis may be associated with the apoptosis pathway.

Conclusions:

The present study illustrated the selectivity of the cytotoxic effect of Hcs against the Т24 cancer cell line. This is the first report of protein expression in T24 human bladder cancer cells under the influence of FU βc-HlH-h. That is probably due to the specific oligosaccharide structures rich in methylated hexoses exposed on the surface of βc-HlH-h.
Observation and Analysis of Clinical Efficacy of Zhuang Medicine Lotus
Acupuncture Cupping Stasis Therapy on Patients with Postherpetic
Neuralgia
Authors: Lai Jingjing , Li Meikang , Li Jie , Han Haitao , Huang Jinming , Qin Hongling , Li Xiujuan , Tan Lei , Xu Jing , Peng Jinxiu
Published: August 2022

Objective:

To explore the clinical efficacy of Zhuang Medicine Lotus Acupuncture
Cupping Stasis Therapy on patients with postherpetic neuralgia (PHN) and its action mechanism.

Methods:

36 patients are randomly divided into Lotus Acupuncture Cupping Stasis Therapy group,
pure cupping group and gabapentin group, with a total of five observation points for the first, fifth,
tenth, fifteenth, and twentieth sessions of therapy (one session every three days). At each observation
point, the venous blood of the patients is taken, and the contents of and changes in WNT3a,
Frizzled8, β-catenin, IL-18, TNF-α, NR2B, NK-1 and SP are tested by ELISA, RT-PCR and WesternBlot,
respectively. The VAS scores and safety of the patients in the three groups are compared.

Results:

With increased time spent in therapy, the VAS scores of patients in each group decreased
gradually and there was a significant reduction in pain in patients in the Lotus Acupuncture Cupping
Stasis Therapy group compared to the gabapentin and pure cupping groups (P<0.05). The levels
of IL-18, TNF-α, NK-1, SP, WNT3a, Frizzled 8 and β-catenin in the serum of all patients experienced
a constant decline over time (P<0.05); the levels of the aforesaid factors in the serum of patients in
the Lotus Acupuncture Cupping Stasis Therapy group dropped remarkably after the tenth session of
therapy compared to those in gabapentin and pure cupping groups (P<0.05).

Conclusions:

Zhuang Medicine Lotus Acupuncture Cupping Stasis Therapy can significantly reduce
the pain of PHN patients, with a good therapeutic effect, and it is worthy of clinical use.
ASF1B, as an Independent Prognostic Biomarker, Correlates with
Immune Infiltrates in Hepatocellular Carcinoma
Authors: Li Renzhi , Cui Xiaohan , Sun Weijun , Yang Zhen , Shen Xingyuan , Zhu Chunfu
Published: August 2022

Background:

Hepatocellular carcinoma (HCC) is one of the fastest-growing malignancies globally. The impact of surgical treatment is limited, and molecular targeted therapy has not yielded a consistent efficacy. This warrants for identification of novel molecular targets. The anti-silencing function of 1B histone chaperone (ASF1B) previously studied in numerous cancers. However, the understanding of its role in HCC is limited.

Methods:

The TIMER database used to analyze the ASF1B expression in pan-cancer and para-carcinoma tissues. ASF1B expression in HCC confirmed by using the HCCDB database, Quantitative real-time PCR (q-PCR), and Western blot (WB) assays. The relationship between clinicopathological parameters and ASF1B expression analyzed by using UALCAN, whereas the prognostic value of ASF1B was evaluated using the GEPIA database. Linkedomics and cBioportal databases used to validate the ASF1B co-expression associated with immune infiltration by the TIMER database. Moreover, cell proliferation after ASF1B-knockdown was determined through CCK8 and clone formation assays.

Results:

ASF1B was highly expressed in HCC tissues and the expression levels were linked to tumor grade, race, and disease stage. Univariate and multivariate Cox models showed that ASF1B is an independent prognostic factor in HCC. CCK8 and clone formation assays demonstrated that ASF1B promotes cell proliferation. Gene co-expression analysis in Linkedomics demonstrated that HJURP, KIF2C, KIF4A, KIF18B, and KIFC1 expression were intimately associated with ASF1B and immune infiltrate cells.

Conclusion:

This study shows that ASF1B promotes the proliferation of HCC. Besides, ASF1B could be a potential prognostic biomarker for HCC patients.
An Update On Proficiency of Voltage-gated Ion Channel Blockers in the
Treatment of Inflammation-associated Diseases
Authors: Godad Angel Pavalu , Kelkar Siddesh , Nailwal Namrata , Bhatia Nirav Yogesh , Doshi Gaurav , Sathaye Sadhana
Published: August 2022

Inflammation is the body’s mechanism to trigger the immune system, thereby preventing bacteria and viruses from manifesting their toxic effect. Inflammation plays a vital role in regulating inflammatory mediator levels to initiate the wound healing process depending on the nature of the stimuli. This process occurs due to chemical release from white blood cells by elevating blood flow to the site of action, leading to redness and increased body temperature. Currently, there are numer-ous Non-steroidal anti-inflammatory drugs (NSAIDs) available, but these drugs are reported with adverse effects such as gastric bleeding, progressive kidney damage, and increased risk of heart at-tacks when prolonged use. For such instances, alternative options need to be adopted. The introduc-tion of voltage-gated ion channel blockers can be a substantial alternative to mask the side effects of these currently available drugs. Chronic inflammatory disorders such as rheumatoid and osteoarthri-tis, cancer and migraine, etc., can cause dreadful pain, which is often debilitating for the patient. The underlying mechanism for both acute and chronic inflammation involves various complex re-ceptors, different types of cells, receptors, and proteins. The working of voltage-gated sodium and calcium channels is closely linked to both inflammatory and neuropathic pain. Certain drugs such as carbamazepine and gabapentin, which are ion channel blockers, have greater pharmacotherapeutic activity for sodium and calcium channel blockers for the treatment of chronic inflammatory pain states. This review intends to provide brief information on the mechanism of action, latest clinical trials, and applications of these blockers in treating inflammatory conditions.
Diabetic Nephropathy: Pathogenesis to Cure
Authors: Gupta Jeena , Kushwaha Kriti , Kabra Uma , Dubey Rupal
Published: August 2022

Diabetic nephropathy (DN) is a leading cause of end-stage renal disorder (ESRD). It is defined as the increase in urinary albumin excretion (UAE) when no other renal disease is present. DN is categorized into microalbuminuria and macroalbuminuria. Factors like high blood pressure, high blood sugar levels, genetics, oxidative stress, hemodynamic and metabolic changes affect DN. Hyperglycemia causes renal damage through activating protein kinase C (PKC), producing advanced end glycation products (AGEs) and reactive oxygen species (ROS). Growth factors, chemokines, cell adhesion molecules, inflammatory cytokines are found to be elevated in the renal tissues of the diabetic patient. Many different and new diagnostic methods and treatment options are available due to the increase in research efforts and progression in medical science. However, until now no permanent cure is available. This article aims to explore the mechanism, diagnosis, and therapeutic strategies in current use for increasing the understanding of DN