bluuebunny/crossref_metadata_2025 huggingface dataset:
An Automated Sprinkler Cooling System Effectively Alleviates Heat Stress in Dairy Cows
Authors: Liu En , Liu Liping , Zhang Zhili , Qu Mingren , Xue Fuguang
Published: September 2024

(1) Background: Heat stress detrimentally restricted economic growth in dairy production. In particular, the cooling mechanism of the spraying system effectively reduced both environmental and shell temperatures. This study was designed to investigate the underlying modulatory mechanism of an automatic cooling system in alleviating heat-stressed dairy cows. (2) Methods: A total of 1208 multiparous dairy cows was randomly allocated into six barns, three of which were equipped with automatic sprinklers (SPs), while the other three were considered the controls (CONs). Each barn was considered a replicate. (3) Results: Body temperatures and milk somatic cell counts significantly decreased, while DMI, milk yield, and milk fat content significantly increased under SP treatment. Rumen fermentability was enhanced, embodied by the increased levels of total VFA, acetate, propionate, and butyrate after SP treatment. The rumen microbiota results showed the relative abundances of fiber-degrading bacteria, including the Fibrobacters, Saccharofermentans, Lachnospira, Pseudobutyrivibrio, Selenomonas, and Succinivibrio, which significantly increased after receiving the SP treatment. (4) Conclusions: This study demonstrated that SP effectively alleviated heat stress and improved production performances and milk quality through modulating the rumen microbiota composition and fermentation function of dairy cows.
The Promising Role of Synthetic Flavors in Advancing Fish Feeding Strategies: A Focus on Adult Female Zebrafish (Danio rerio) Growth, Welfare, Appetite, and Reproductive Performances
Authors: Conti Federico , Olivotto Ike , Cattaneo Nico , Pavanello Massimiliano , Şener İdris , Antonucci Matteo , Chemello Giulia , Gioacchini Giorgia , Zarantoniello Matteo
Published: September 2024

The present study aimed to test over a six-month period different synthetic flavors in zebrafish (Danio rerio) as an experimental model. Specifically, two attractive and one repulsive synthetic flavors were added (1% w/w) to a specific zebrafish diet, which was administered to the fish during the whole life cycle (from larvae to adults), to evaluate their physiological responses, emphasizing fish welfare, feed intake, growth, reward mechanisms, and reproductive performances. Fish welfare was not affected by all tested flavors, while both attractive flavors promoted fish feed ingestion and growth. The results were supported by both molecular and immunohistochemical analyses on appetite-regulating neurohormonal signals, along with the influence of the feed hedonic properties induced by the brain reward sensation, as demonstrated by the dopamine receptor gene expression. Finally, the present study demonstrated that a higher feed intake also had positive implications on fish reproductive performances, suggesting a promising role of synthetic flavors for the aquaculture industry. In conclusion, the results highlighted the potential of synthetic flavors to improve fish feeding strategies by providing a consistent and effective alternative to traditional stimulants, thereby reducing dependence on natural sources.
Precision Dosing of Meropenem in Adults with Normal Renal Function: Insights from a Population Pharmacokinetic and Monte Carlo Simulation Study
Authors: Kim Yong Kyun , Kang Gaeun , Zang Dae Young , Lee Dong Hwan
Published: September 2024

This study aimed to develop a population pharmacokinetic (PK) model for meropenem in healthy adults and explore optimal dosing regimens for patients with normal renal function. PK samples were obtained from 12 healthy participants, which were analyzed using noncompartmental analysis and nonlinear mixed-effect modeling. The PK profiles of meropenem were characterized using a two-compartment model, and serum creatinine level was identified as a significant covariate affecting total clearance. Monte Carlo simulations were conducted using this model to inform dosing recommendations. The target index for meropenem efficacy was defined as the cumulative percentage over 24 h during which free (f) drug concentration exceeded the minimum inhibitory concentration (MIC) under steady state conditions (fT>MIC). These simulations indicated that the current dosage regimen of 1 g for 30 min infusions every 8 h achieved a 90% probability of target attainment (PTA) for 40%fT>MIC when the MIC was <2 mg/L. However, to achieve more stringent therapeutic targets, such as a 90%PTA for 100%fT>MIC or a 90%PTA for 100%fT>4MIC, higher doses administered as 3 h extended infusions or as continuous infusions may be necessary. These results highlight the need for model-informed precision dosing to enhance the efficacy of meropenem therapy across various MIC levels and therapeutic targets.
Robust Text-to-Cypher Using Combination of BERT, GraphSAGE, and Transformer (CoBGT) Model
Authors: Tran Quoc-Bao-Huy , Waheed Aagha Abdul , Chung Sun-Tae
Published: September 2024

Graph databases have become essential for managing and analyzing complex data relationships, with Neo4j emerging as a leading player in this domain. Neo4j, a high-performance NoSQL graph database, excels in efficiently handling connected data, offering powerful querying capabilities through its Cypher query language. However, due to Cypher’s complexities, making it more accessible for nonexpert users requires translating natural language queries into Cypher. Thus, in this paper, we propose a text-to-Cypher model to effectively translate natural language queries into Cypher. In our proposed model, we combine several methods to enable nonexpert users to interact with graph databases using the English language. Our approach includes three modules: key-value extraction, relation–properties prediction, and Cypher query generation. For key-value extraction and relation–properties prediction, we leverage BERT and GraphSAGE to extract features from natural language. Finally, we use a Transformer model to generate the Cypher query from these features. Additionally, due to the lack of text-to-Cypher datasets, we introduced a new dataset that contains English questions querying information within a graph database, paired with corresponding Cypher query ground truths. This dataset aids future model learning, validation, and comparison on text-to-Cypher task. Through experiments and evaluations, we demonstrate that our model achieves high accuracy and efficiency when comparing with some well-known seq2seq model such as T5 and GPT2, with an 87.1% exact match score on the dataset.
Adam Bayesian Gaussian Process Regression with Combined Kernel-Function-Based Monte Carlo Reliability Analysis of Non-Circular Deep Soft Rock Tunnel
Authors: Xu Jiancong , Yan Ziteng , Wang Yongshuai
Published: September 2024

Evaluating the reliability of deep soft rock tunnels is a very important issue to be solved. In this study, we propose a Monte Carlo simulation reliability analysis method (MCS–RAM) integrating the adaptive momentum stochastic optimization algorithm (Adam), Bayesian inference theory and Gaussian process regression (GPR) with combined kernel function, and we developed it in Python. The proposed method used the Latin hypercube sampling method to generate a dataset sample of geo-mechanical parameters, constructed combined kernel functions of GPR and used GPR to establish a surrogate model of the nonlinear mapping relationship between displacements and mechanical parameters of the surrounding rock. Adam was used to optimize the hyperparameters of the surrogate model. The Bayesian inference algorithm was used to obtain the probability distribution of geotechnical parameters and the optimal surrounding rock mechanical parameters. Finally, the failure probability was computed using MCS–RAM based on the optimized surrogate model. Through the application of an engineering case, the results indicate that the proposed method has fewer prediction errors and stronger prediction ability than Kriging or XGBoost, and it can significantly save computational time compared with the traditional polynomial response surface method. The proposed method can be used in the reliability analysis of all shapes of tunnels.
Assessment of Driver’s Head Acceleration during a Possible Car Skidding Effect
Authors: Martínez-Miranda Miguel Ángel , Yamamoto Yosuke , Yasunaga Shun , Kan Tetsuo , Espinoza-Garcés Carlos Alberto , Silva-Garcés Karla Nayeli , Torres-SanMiguel Christopher Rene
Published: September 2024

This document provides a design description of a data acquisition device that allows an alert to be issued to suggest to the driver to take a break after having subjected his body to a certain amount of acceleration and pressure changes after driving on a road with too many curves. The tests were carried out using sensors based on microelectromechanical systems. The system was strategically installed at specific points on the body of the driver and car. Several electronic arrays were carried out, like the design of a printed circuit board. The establishment of an inter-integrated circuit communication and its multiplexing to work with several devices with the same address simultaneously. Finally, in this context, the document also presents the critical velocity for each curve in the Hakone roadway, which was obtained by using a mathematical model and contrasted with data acquisition values for acceleration. The risk of skidding on a curve increases when the driver does not reduce driving velocity; only a slight variance in acceleration or environmental conditions is enough. The value of acceleration was acquired for the analysis of each curve; there is a greater possibility of skidding in curves 2 and 4 because their radius is smaller and the critical speed is approximately 60 km/h, which is very close to the driving speed. On the other hand, the deceleration value of −0.65 G read on the head accelerometer can increase fatigue symptoms such as blurry vision or dizziness.
Chemicals from Brominated Flame Retardants: Analytical Methods, Occurrence, Transport and Risks
Authors: Enyoh Christian Ebere , Maduka Tochukwu Oluwatosin , Rana Md. Sohel , Osigwe Sochi Chinaemerem , Ihenetu Stanley Chukwuemeka , Wang Qingyue
Published: September 2024

Brominated flame retardants (BFRs) are synthetic chemicals widely used to reduce the flammability of consumer products, including electronics, textiles, and furniture. Despite their effectiveness in fire prevention, BFRs pose significant environmental and health risks due to their persistence, bioaccumulation, and potential toxicity. This review provides a comprehensive examination of BFRs, focusing on recent advancements in analytical methods for their detection and quantification in environmental and biological samples. The study explored the physicochemical properties that influence BFR distribution and transport in various matrices, including soil, water, air, sediments, and biota. The review also summarizes current knowledge on the occurrence and environmental fate of BFRs, highlighting their mobility and long-range transport. Furthermore, the study discusses the health risks associated with BFR exposure, emphasizing their endocrine-disrupting effects and impact on reproductive and neurological functions. By integrating findings from recent studies, this review aims to enhance the understanding of BFR behavior and inform regulatory strategies to mitigate their adverse effects on human health and the environment.
Simulated Seismicity as a Tool for Studying the Long-Term Seismogenic Process: An Italy–Japan Comparison
Authors: Console Rodolfo , Carluccio Roberto , Vannoli Paola
Published: September 2024

In this study, we aimed to assess the capacity of a physics-based earthquake simulator to improve our understanding of the seismogenic process. In this respect, we applied a previously tested earthquake simulator to two well-known and completely different seismogenic fault systems, namely the Italian Apennines and the Nankai subduction in Japan, for which long historical records of strong earthquakes are available. They are characterized by different fault mechanisms, fault sizes, and slip rates. Because of the difference in slip rates, the time scale of the seismicity patterns is different for the two systems (several hundreds of years for the Apennines and a few tens of years for the Nankai Fault). The results of simulations that produced synthetic catalogues of 100,000 years show these significant long-term seismicity patterns characterizing the seismic cycles for both seismogenic areas as follows: The average stress and the occurrence rate of earthquakes increase in the long term as the next major earthquake approaches; while the average stress increases uniformly, the occurrence rate stops increasing well in advance of the mainshocks; the b-value exhibits a long-term increase before major earthquakes and a fast decrease shortly before the mainshocks. Even if no specific statistical tool was applied for the quantification of the similarities between the seismicity patterns of the two seismic areas, such similarities are clearly justified by the large number of seismic cycles included in the 100,000-year synthetic catalogues. The paper includes a discussion on the capability of the simulation algorithm to reliably represent the real long-term seismogenic process. This question is difficult to answer because the available historical observations are of too short a duration to provide significant statistical results. In spite of the limitations characterizing the use of earthquake simulators for time-dependent earthquake hazard assessment, and the lack of convincing mechanistic explanations of the specific seismic patterns reproduced by our simulator algorithm, our results encourage further investigations into the application of simulators for the development of seismogenic models, including short-term features.
Does the Transcranial Direct Current Stimulation Selectively Modulate Prefrontal Cortex Hemodynamics? An Immediate Effect-Controlled Trial on People with and without Depression
Authors: Campos Laura Oliveira , Macedo Maria de Cassia Gomes Souza , Monerat Vheyda Katheleen Vespasiano , Ferreira Kariny Realino do Rosário , Santos Mayra Evelise Cunha dos , Esquirio Arthur Ferreira , Alves Ana Luiza Guimarães , Gama Gabriela Lopes , Barbosa Michelle Almeida , Barbosa Alexandre Carvalho
Published: September 2024

Despite the recommendation to treat depression using transcranial direct current stimulation (tDCS), novel findings raise doubts over the tDCS’s efficacy in managing depressive episodes. Neurophysiologic approaches to understanding the specificities of brain responses to tDCS in patients with depression remain to be explored. Objective: Our aim was to compare immediate hemodynamic responses to tDCS on the left dorsolateral prefrontal cortex (DLPFC; F3-Fp2 montage) in patients with depressive disorder and in controls (no additional stimuli). Methods: Sixteen participants were allocated to the depression group and sixteen to the control group. Both groups received 2 mA tDCS for 20 min, using the F3-Fp2 montage. The hemodynamic effect over the DLPFC was assessed using functional near-infrared intracranial spectroscopy (fNIRS) positioned on the left supraorbital region (Fp1). Mean, minimal, and maximal values of baseline and post-stimulation rates of oxygen saturation (SatO2) were recorded. The oxygenated hemoglobin rates (HbO) were extracted. Results: Between-group differences were detected for minimal baseline rates of SatO2 and HbO levels. The depression group showed lower results compared to the control group at baseline. After the protocol, only the depression group showed increased minimal rates of SatO2 and HbO. The post-tDCS minimal rates were equal for both groups. Conclusions: The findings showed immediate anodal tDCS effects over DLPFC hemodynamics. The effects were exclusive to the lowest baseline rate group and did not affect the normal oxygen rate group. The minimal increase in SatO2 and HbO rates after the protocol in the depression group suggests that those with reduced cerebral perfusion may be more affected by tDCS.
EGS-YOLO: A Fast and Reliable Safety Helmet Detection Method Modified Based on YOLOv7
Authors: Han Jianfeng , Li Zhiwei , Cui Guoqing , Zhao Jingxuan
Published: September 2024

Wearing safety helmets at construction sites is a major measure to prevent safety accidents, so it is essential to supervise and ensure that workers wear safety helmets. This requires a high degree of real-time performance. We improved the network structure based on YOLOv7. To enhance real-time performance, we introduced GhostModule after comparing various modules to create a new efficient structure that generates more feature mappings with fewer linear operations. SE blocks were introduced after comparing several attention mechanisms to highlight important information in the image. The EIOU loss function was introduced to speed up the convergence of the model. Eventually, we constructed the efficient model EGS-YOLO. EGS-YOLO achieves a mAP of 91.1%, 0.2% higher than YOLOv7, and the inference time is 13.3% faster than YOLOv7 at 3.9 ms (RTX 3090). The parameters and computational complexity are reduced by 37.3% and 33.8%, respectively. The enhanced real-time performance while maintaining the original high precision can meet actual detection requirements.
Leveraging Gamification in ICT Education: Examining Gender Differences and Learning Outcomes in Programming Courses
Authors: Mellado Rafael , Cubillos Claudio , Vicari Rosa Maria , Gasca-Hurtado Gloria
Published: September 2024

This study investigates the differential effects of gamification on learning outcomes, motivation, and usability perceptions in an introductory programming course, focusing on gender differences. While gamification has shown promise for increasing student engagement in educational settings, its impact may vary across genders. An experimental study was conducted with 88 university students randomly assigned to gamified and non-gamified groups. Learning gains were assessed through pre- and post-tests, motivational factors were measured via questionnaires, and usability perceptions were evaluated using the Technology Acceptance Model (TAM) questionnaire. Results revealed that women learned significantly more than men in the non-gamified condition, while men outperformed women in the gamified condition. Furthermore, men reported higher enjoyment, usefulness, and comfort with the gamified tool than women. Interestingly, both genders indicated greater satisfaction with the non-gamified version. These findings contribute nuanced insights into how gamification impacts genders differently in programming education, suggesting that gamification may hinder women’s learning while modestly benefiting men. The study highlights the importance for practitioners to carefully consider gender dynamics when implementing gamified approaches, potentially offering customization options or blended techniques to optimize learning outcomes for all students in programming education.
Static and Dynamic Analysis of Bidirectionally Sinusoidal Corrugated Steel Shells—Comparative FEA Study
Authors: Kozanecki Damian , Wirowski Artur , Rabenda Martyna
Published: September 2024

This study investigates bidirectionally sinusoidal corrugated steel shells using finite element analysis (FEA) software, primarily ABAQUS 2017, supplemented by RFEM 6.05 for an initial result comparison. The research aims to establish a robust numerical solution to understand the structural behaviour of these shells under static and dynamic loading. Starting with meticulous calculations for a selected structural element, the paper emphasises a comparative analysis between ABAQUS and RFEM, offering valuable insights into simulating the responses of corrugated steel shells. A distinctive contribution of this research lies in its novel and comprehensive parametric analysis of these shells under static and dynamic loading, an area that has not been explored in previous studies. The study systematically explores various model parameters, including geometrical and mechanical properties, with detailed analyses revealing their influences on deformation, stress distribution, dynamic properties, and behaviour. Another key feature is the development of a coded script that systematically generates diverse numerical models, allowing for a thorough exploration of the structural system’s response. This study advances our understanding of the structural behaviour of bidirectionally sinusoidal corrugated steel shells. These findings are set to enhance structural analysis and design practices by optimising parameters for improved performances in various engineering applications.
On a Generic Fractional Derivative Associated with the Riemann–Liouville Fractional Integral
Authors: Luchko Yuri
Published: September 2024

In this paper, a generic fractional derivative is defined as a set of the linear operators left-inverse to the Riemann–Liouville fractional integral. Then, the theory of the left-invertible operators developed by Przeworska-Rolewicz is applied to deduce its properties. In particular, we characterize its domain, null-space, and projector operator; establish the interrelations between its different realizations; and present a generalized fractional Taylor formula involving the generic fractional derivative. Then, we consider the fractional relaxation equation containing the generic fractional derivative, derive a closed-form formula for its unique solution, and study its complete monotonicity.
Cloning and Functional Analysis of a Zeaxanthin Epoxidase Gene in Ulva prolifera
Authors: He Hongyan , Yang Xiuwen , Zeb Aurang , Liu Jiasi , Gu Huiyue , Yang Jieru , Xiang Wenyu , Shen Songdong
Published: September 2024

The xanthophyll cycle is a photoprotective mechanism in plants and algae, which protects the photosynthetic system from excess light damage under abiotic stress. Zeaxanthin is considered to play a pivotal role in this process. In this study, the relative content of xanthophylls was determined using HPLC-MS/MS in Ulva prolifera exposed to different salinities. The results showed that high-salt stress significantly increased the relative content of xanthophylls and led to the accumulation of zeaxanthin. It was speculated that the accumulated zeaxanthin may contribute to the response of U. prolifera to high-salt stress. Zeaxanthin epoxidase (ZEP) is a key enzyme in the xanthophyll cycle and is also involved in the synthesis of abscisic acid and carotenoids. In order to explore the biological function of ZEP, a ZEP gene was cloned and identified from U. prolifera. The CDS of UpZEP is 1122 bp and encodes 373 amino acids. Phylogenetic analysis showed that UpZEP clusters within a clade of green algae. The results of qRT-PCR showed that high-salt stress induced the expression of UpZEP. In addition, heterologous overexpression of the UpZEP gene in yeast and Chlamydomonas reinhardtii improved the salt tolerance of transgenic organisms. In conclusion, the UpZEP gene may be involved in the response of U. prolifera to high-salt stress and can improve the high-salt tolerance of transgenic organisms.
The Role of Chemokines in Obesity and Exercise-Induced Weight Loss
Authors: He Wenbi , Wang Huan , Yang Gaoyuan , Zhu Lin , Liu Xiaoguang
Published: September 2024

Obesity is a global health crisis that is closely interrelated to many chronic diseases, such as cardiovascular disease and diabetes. This review provides an in-depth analysis of specific chemokines involved in the development of obesity, including C-C motif chemokine ligand 2 (CCL2), CCL3, CCL5, CCL7, C-X-C motif chemokine ligand 8 (CXCL8), CXCL9, CXCL10, CXCL14, and XCL1 (lymphotactin). These chemokines exacerbate the symptoms of obesity by either promoting the inflammatory response or by influencing metabolic pathways and recruiting immune cells. Additionally, the research highlights the positive effect of exercise on modulating chemokine expression in the obese state. Notably, it explores the potential effects of both aerobic exercises and combined aerobic and resistance training in lowering levels of inflammatory mediators, reducing insulin resistance, and improving metabolic health. These findings suggest new strategies for obesity intervention through the modulation of chemokine levels by exercise, providing fresh perspectives and directions for the treatment of obesity and future research.
Oxidative Stress and Asprosin Levels in Type 2 Diabetic Patients with Good and Poor Glycemic Control
Authors: Senyigit Abdulhalim , Durmus Sinem , Gelisgen Remise , Uzun Hafize
Published: September 2024

Objectives: HbA1c is the most widely used test as an indicator of glucoregulation in patients with type 2 diabetes mellitus (T2DM). Asprosin and oxidative stress levels can be reduced with good glycemic control (GC) and thus prevented or delayed micro/macro complications in patients with T2DM. The relationship between asprosin, which is thought to affect GC, and oxidative stress parameters such as lipid hydroperoxides (LOOHs), glutathione (GSH), malondialdehyde (MDA), superoxide dismutase (Cu,Zn-SOD), and total antioxidant capacity (TAC) was evaluated in T2DM patients. Materials and Methods: The study was conducted prospectively in 75 healthy people admitted to the hospital for a general health check-up and 150 T2DM patients treated in the diabetes outpatient clinic. The patient’s glycemic status measurements were categorized as good glycemic control group (GGC) is defined as HbA1c < 7 and poor glycemic control (PGC) group is defined as HbA1c ≥ 7. Results: The study found a consistent increase in LOOH and MDA levels across the control, GGC, and PGC groups, while GSH, Cu/Zn-SOD, and TAC levels decreased in these respective groups. Additionally, asprosin levels showed a gradual rise in all groups. Positive correlations were observed between asprosin levels and various metabolic and oxidative stress markers, including BMI, WC, FBG, insulin, homeostasis model assessment for insulin resistance (HOMA-IR), DM duration, LOOH, and MDA, while negative correlations were noted with GSH, Cu/Zn-SOD, and TAC specifically in the PGC group. Furthermore, multivariate regression analysis identified HOMA-IR as the primary influencing factor on asprosin levels in PGC patients. Conclusions: Current glycemic dysregulation may lead to increased circulating asprosin and oxidative stress, which cause complications. Since asprosin levels may be an important hormonal factor in determining GC in T2DM, the use of this hormone may be recommended in the future to accelerate therapeutic approaches in T2DM. Early diagnosis and appropriate treatment may delay the development and progression of diabetic complications.
Sorghum Biomass as an Alternative Source for Bioenergy
Authors: Morales Marina Moura , Hoshide Aaron Kinyu , Carvalho Leticia Maria Pavesi , Tardin Flavio Dessaune
Published: September 2024

Alternative biomass for energy can reduce fossil fuel use and environmental impacts, providing energy security in semi-arid areas with shallow soils that are not ideal for agro-forestry. The densification of sorghum biomass (SB) brings its energetic characteristics closer those of wood. Higher heating value (HHV) represents the heat produced by a given quantity of fuel. This Brazilian research tested different mixtures of SB, eucalyptus wood (W), and eucalyptus bio-oil (Bo) as briquettes for HHV and least ash. Compressed mixtures of SB+B were compared to W+Bo and SB+W+Bo. The concentrations of bio-oil added to SB/W were 1%, 3%, 4%, and 5%. SB+W+Bo composites’ W content was 0%, 25%, 50%, 75%, and 100%, with Bo as 3% of the weight. Sorghum biomass’ HHV is equivalent to W at 3%Bo. Bo doses of 4% and 5% had the same HHV as 3%. Eucalyptus wood did not have a significantly greater HHV with any amount of Bo. SB+W+3%Bo had the same HHV as W when W was at least 50% of the mixture. At greater than 36%W, the ash content was lower than 3%, meeting the EN-B international standard. The optimal composite mixture was 64%SB+36%W+3%Bo for HHV and ash content. SB briquettes can be more widely adopted given sorghum’s prevalence in semi-arid environments.
Are Sirtuins 1 and 2 Relevant Players in Relapsing–Remitting Multiple Sclerosis?
Authors: Chojdak-Łukasiewicz Justyna , Bizoń Anna , Kołtuniuk Aleksandra , Waliszewska-Prosół Marta , Budrewicz Sławomir , Piwowar Agnieszka , Pokryszko-Dragan Anna
Published: September 2024

SIRTs were demonstrated to play an important role in inflammatory, degenerative, and metabolic alterations, constituting the background of the central nervous system. Thus, they seem to be an appropriate object of investigation (as potential biomarkers of disease activity and/or novel therapeutic targets) in multiple sclerosis (MS), which has a complex etiology that comprises a cross-talk between all these processes. The aim of this study was to evaluate the levels of SIRT1 and SIRT2 in the serum of patients with the relapsing–remitting type of MS (RRMS), as well as their relationships with various aspects of MS-related disability. Methods: A total of 115 patients with RRMS (78 women, 37 men, mean age 43 ± 9.9) and 39 healthy controls were included in the study. SIRT1 and SIRT2 were detected in the serum using the enzyme-linked immunoassay (ELISA) method. In the RRMS group, relationships were investigated between the SIRT 1 and 2 levels and the demographic data, MS-related clinical variables, and the results of tests evaluating fatigue, sleep problems, cognitive performance, autonomic dysfunction, and depression. Results: The levels of SIRT1 and SIRT2 in RRMS patients were significantly lower than in the controls (11.14 vs. 14. 23, p = 0.04; 8.62 vs. 14.2, p < 0.01). In the RRMS group, the level of both SIRTs was higher in men than in women (15.7 vs. 9.0; 11.3 vs. 7.3, p = 0.002) and showed a significant correlation with the degree of disability (R = −0.25, p = 0.018). No other relationships were found between SIRT levels and the analyzed data. Conclusions: The serum levels of SIRT1 and 2 were decreased in the RRMS patients (especially in the female ones) and correlated with the degree of neurological deficit. The role of SIRTs as biomarkers of disease activity or mediators relevant for “invisible disability” in MS warrants further investigation.
Biological, Antifungal, and Physical Efficacy of a Denture Cleanser Formulated with Cnidium officinale Extracts
Authors: Lee Myung-Jin , Yang Song-Yi , Kang Min-Kyung
Published: September 2024

Background/Objectives: We aimed to assess the antifungal efficacy and impact of a denture cleanser containing Cnidium officinale extract on the surface characteristics of denture base materials, as well as its physical and biological properties. Methods: The experimental denture cleansers were formulated with C. officinale at concentrations of 100 and 150 μg/mL, combined with 1% cocamidopropyl betaine as a natural surfactant. Antifungal efficacy was evaluated using zone-of-inhibition assays against Candida albicans, revealing inhibition zones of 20 ± 1.8 mm for the 100 μg/mL concentration and 23.6 ± 1.6 mm for the 150 μg/mL concentration. Surface property assessments—including hardness, roughness, color stability, and solubility measurements—demonstrated no significant differences compared to the control group. Biological evaluations included the quantification of polyphenol and flavonoid content. Results: The C. officinale-based cleanser showed significant antifungal activity without affecting the hardness, roughness, color stability, or solubility of denture base materials. Biological tests revealed no cytotoxicity and minimal mucosal irritation. Polyphenol and flavonoid contents were quantitatively measured, revealing higher concentrations in the experimental groups, which were correlated with significant antifungal activity. These compounds are known for their roles in disrupting microbial processes and enhancing antimicrobial effects. These findings suggest that the C. officinale-based denture cleanser effectively inhibits C. albicans while preserving the physical properties of denture base materials. Conclusions: This study highlights the potential of C. officinale in denture cleanser formulations, promoting denture hygiene and oral health. Future research should prioritize long-term clinical evaluations and formulation optimization.
Subclinical Left Ventricular Dysfunction over Seven-Year Follow-Up in Type 2 Diabetes Patients without Cardiovascular Diseases
Authors: Akasheva Dariga Uaydinichna , Utina Tatyana Gennadyevna , Dzhioeva Olga Nikolaevna , Drapkina Oxana Mikhailovna
Published: September 2024

Subclinical left ventricular dysfunction (LVD) is common in asymptomatic patients with type 2 diabetes (T2D). This study aimed to define long-term structural and functional disorders of the left ventricle (LV) myocardium over a 7-year follow-up in patients with T2D without cardiovascular diseases (CVD). Of the 120 patients with and without T2D of both sexes aged from 45 to 75 years (57.11 ± 7.9 years), included in the study in 2012–2013, 57 responded to the follow-up study. They were divided into two groups: one with T2D (n = 29), the other without it, the control (n = 28). All patients underwent transthoracic two-dimensional echocardiography with an assessment of standard indicators of systolic and diastolic cardiac function, global longitudinal strain (GLS), laboratory diagnostics of carbohydrate metabolism disorders markers, NT-proBNP, and CRP. The median follow-up duration was 7.2 [7.0–7.8] years. During the follow-up, a statistically significant increase in the incidence of diastolic dysfunction (DD) from 53% to 61% (p = 0.004) was found in the T2D group; no significant dynamics were noted in the control group (p = 0.48). The proportion of patients with reduced GLS (<−18%) increased in the T2D group (p = 0.036). A significant difference in the frequency of decreased GLS depending on presence of T2D was demonstrated. In conclusion, T2D is an independent risk factor for the worsening of subclinical left ventricular dysfunction in asymptomatic patients with T2D without CVD over 7-year follow-up.
Evaluation of Psychometric Proprieties of Mann Assessment of Swallowing Ability (MASA) in Italian Language: A Cross-Sectional Study
Authors: Sterbini Arianna , Marroni Patrizia , Servadio Annamaria , Rossi Giulia , Berardi Anna , Simeon Rachele , Galeoto Giovanni
Published: September 2024

The aim of this cross-sectional study was to translate, culturally adapt, and evaluate the psychometric properties of the Italian version of the Mann Assessment of Swallowing Ability (MASA) in post-stroke individuals. The original MASA scale was translated and culturally adapted from English to Italian following the international guidelines. The internal consistency and test–retest reliability of the MASA-IT were assessed, and its concurrent validity was examined through Pearson correlation coefficients with the Italian versions of two established gold standard scales for dysphagia assessment: the Dysphagia Outcome Severity Scale (DOSS) and the De Pippo Test—Three Oz Water Swallow. The MASA-IT was administered to 78 participants. The items demonstrated excellent internal consistency, with Cronbach’s alpha ranging between 0.86 and 0.89. The interclass correlation coefficient was 0.98 for inter-rater reliability and 0.99 for intra-rater reliability, indicating high reproducibility. Regarding concurrent validity, the MASA-IT showed a strong direct correlation with the DOSS scale (r = 0.949, p = 0.01) and an inverse correlation with the De Pippo Test (r = −0.783, p = 0.01), confirming its good concurrent validity. The Italian version of the Mann Assessment of Swallowing Ability (MASA-IT) is a reliable and valid tool for assessing swallowing ability in post-stroke patients. Its strong psychometric properties make it well suited for clinical use in Italy.
The Influence of Parents’ Educational Expectations on Children’s Development: The Chain Mediation Role of Educational Anxiety and Parental Involvement
Authors: Xin Ye , Yu Lu
Published: September 2024

In the social context of the “Educational Involution”, the educational expectations of parents have a potential influence on the development of children. High parental educational expectations create parental anxiety, which in turn results in a rise in parental involvement and eventually promotes the growth and progress of children. The current study administered an electronic questionnaire to 891 parents of young children in four provinces of China. The questionnaire included the Parental Educational Expectations Scale, the Educational Anxiety Scale, the Parental Involvement Scale, and the Child Development Scale. This study used SPSS 27.0 for statistical data analysis and the SPSS macro program PROCESS to explore the mediation role. We found that (1) educational anxiety plays a mediating role between parental educational expectations and child development; (2) parental involvement has a mediating effect between parental educational expectations and child development; and (3) educational anxiety and parental involvement play a chain mediating role between parental educational expectations and child development. In conclusion, parental educational expectations appear to contribute to child development, and this effect may be mediated individually and sequentially by educational anxiety and parental involvement.
Towards an Evidence-Based Critical Incidents and Suicides Response Program in Australian Construction
Authors: Biggs Amanda , Townsend Keith , Loudoun Rebecca , Robertson Adam , Mason Jemima , Maple Myfanwy , Lacey James , Thompson Nicholas
Published: September 2024

Fatal and non-fatal accidents and suicides at work pose a substantial threat to workers’ physical and psychological safety, particularly within the construction industry. Managing these incidents is an essential component of workplace health and safety (WHS). Additionally, a formal program to support workers and provide feedback to improve the existing WHS management system is increasingly important. However, knowledge of the factors contributing to an effective critical incident (CI) or postvention response is limited by the lack of published evidence-based interventions, especially for occupations and industries that are exposed to higher rates of fatal and non-fatal injuries due to accidents and suicides. In addition, broader concerns surrounding the effectiveness of conventional critical incident programs highlight the need to develop innovative and evidence-based critical intervention and postvention responses addressing acute stress symptoms arising from exposure. This research outlines the development of the MATES Respond Training Program, a component of the WHO-endorsed MATES in Construction program, which is a charity charged with the task of improving mental health and suicide prevention in the construction industry in Australia and New Zealand. Development of the MATES Respond Training Program was informed by the following three key sources: a rapid literature review, interviews with 11 workers trained in peer support suicide prevention interventions, and an analysis of site notes on 193 critical incident and postvention construction site attendances by MATES in Construction field officers, case managers and social workers. Insights and recommendations obtained from these sources, along with the resulting program, are discussed in this paper.
Biological Functions and Therapeutic Potential of NAD+ Metabolism in Gynecological Cancers
Authors: Myong Subin , Nguyen Anh Quynh , Challa Sridevi
Published: September 2024

Nicotinamide adenine dinucleotide (NAD+) is an important cofactor for both metabolic and signaling pathways, with the dysregulation of NAD+ levels acting as a driver for diseases such as neurodegeneration, cancers, and metabolic diseases. NAD+ plays an essential role in regulating the growth and progression of cancers by controlling important cellular processes including metabolism, transcription, and translation. NAD+ regulates several metabolic pathways such as glycolysis, the citric acid (TCA) cycle, oxidative phosphorylation, and fatty acid oxidation by acting as a cofactor for redox reactions. Additionally, NAD+ acts as a cofactor for ADP-ribosyl transferases and sirtuins, as well as regulating cellular ADP-ribosylation and deacetylation levels, respectively. The cleavage of NAD+ by CD38—an NAD+ hydrolase expressed on immune cells—produces the immunosuppressive metabolite adenosine. As a result, metabolizing and maintaining NAD+ levels remain crucial for the function of various cells found in the tumor microenvironment, hence its critical role in tissue homeostasis. The NAD+ levels in cells are maintained by a balance between NAD+ biosynthesis and consumption, with synthesis being controlled by the Preiss–Handler, de novo, and NAD+ salvage pathways. The primary source of NAD+ synthesis in a variety of cell types is directed by the expression of the enzymes central to the three biosynthesis pathways. In this review, we describe the role of NAD+ metabolism and its synthesizing and consuming enzymes’ control of cancer cell growth and immune responses in gynecologic cancers. Additionally, we review the ongoing efforts to therapeutically target the enzymes critical for NAD+ homeostasis in gynecologic cancers.
Exposed Phosphatidylserine as a Biomarker for Clear Identification of Breast Cancer Brain Metastases in Mouse Models
Authors: Wang Lulu , Zhao Alan H. , Arledge Chad A. , Xing Fei , Chan Michael D. , Brekken Rolf A. , Habib Amyn A. , Zhao Dawen
Published: September 2024

Brain metastasis is the most common intracranial malignancy in adults. The prognosis is extremely poor, partly because most patients have more than one brain lesion, and the currently available therapies are nonspecific or inaccessible to those occult metastases due to an impermeable blood–tumor barrier (BTB). Phosphatidylserine (PS) is externalized on the surface of viable endothelial cells (ECs) in tumor blood vessels. In this study, we have applied a PS-targeting antibody to assess brain metastases in mouse models. Fluorescence microscopic imaging revealed that extensive PS exposure was found exclusively on vascular ECs of brain metastases. The highly sensitive and specific binding of the PS antibody enables individual metastases, even micrometastases containing an intact BTB, to be clearly delineated. Furthermore, the conjugation of the PS antibody with a fluorescence dye, IRDye 800CW, or a radioisotope, 125I, allowed the clear visualization of individual brain metastases by optical imaging and autoradiography, respectively. In conclusion, we demonstrated a novel strategy for targeting brain metastases based on our finding that abundant PS exposure occurs on blood vessels of brain metastases but not on normal brain, which may be useful for the development of imaging and targeted therapeutics for brain metastases.
A Comprehensive Analysis of Skin Cancer Concerns and Protective Practices in Manitoba, Canada, Highlights Lack of Skin Cancer Awareness and Predominance of High-Risk Sun Exposure Behaviors
Authors: Lagacé François , Conte Santina , Mija Lorena A. , Moustaqim-Barrette Amina , Mahmood Farhan , LeBeau Jonathan , McKenna Alyson , Maazi Mahan , Hanna Johnny , Kelly Alexandra Sarah Victoria , Rahme Elham , Hrubeniuk Travis J. , Peláez Sandra , Litvinov Ivan V.
Published: September 2024

The rapidly increasing skin cancer rates in Canada are alarming, with current data estimating that 1/3 of Canadians will be affected in their lifetime. Thus, deeper understanding of high-risk sun exposure behaviors is needed to help counter this trend. Only limited action has been taken by federal/provincial governments to reduce skin cancer incidence. A cross-sectional survey study was conducted in Manitoba, with frequency counts, means, and percentages used to encapsulate responses. Age- and gender-adjusted odds ratios were calculated using logistic regression analyses. Our study identified worrying inadequacies in sun protective behaviors and attitudes, with the threat of such high-risk behaviors amplified by a lack of skin cancer awareness. Alarming elements were noted in participants’ sun exposure history (>65% reported a history of sunburns, >50% previously used a tanning bed, and >75% recently tanned for pleasure), beliefs and attitudes (>50% believe that they look better/healthier with a tan, and >40% believe that having a base tan is protective against further sun damage), and sun protection efforts (sun protective clothing was used <60% of the time, sunscreen was used by <50%, and there was a lack of knowledge about sunscreen characteristics in ~30% of respondents), in addition to significant differences being established between demographic subgroups (based on gender, age, skin phototype, income, and education attained). This study provides worrisome insight onto the grim landscape of sun protective behaviors and attitudes in Manitoba, which will inevitably translate into higher skin cancer rates and should serve as a call to action to promote targeted public health messaging in this jurisdiction and beyond.
A Review of Pathophysiology and Therapeutic Strategies Targeting TGF-β in Graves’ Ophthalmopathy
Authors: Chang Hsin-Ho , Wu Shi-Bei , Tsai Chieh-Chih
Published: September 2024

TGF-β plays a pivotal role in the pathogenesis of GO by promoting orbital tissue remodeling and fibrosis. This process involves the stimulation of orbital fibroblasts, leading to myofibroblast differentiation, increased production of inflammatory mediators, and hyaluronan accumulation. Studies have elucidated TGF-β’s role in driving fibrosis and scarring processes through both canonical and non-canonical pathways, particularly resulting in the activation of orbital myofibroblasts and the excessive accumulation of extracellular matrix. Additionally, recent in vitro and in vivo studies have been summarized, highlighting the therapeutic potential of targeting TGF-β signaling pathways, which may offer promising treatment interventions for GO. This review aims to consolidate the current understanding of the multifaceted role of TGF-β in the molecular and cellular pathophysiology in Graves’ ophthalmopathy (GO) by exploring its contributions to fibrosis, inflammation, and immune dysregulation. Additionally, the review investigates the therapeutic potential of inhibiting TGF-β signaling pathways as a strategy for treating GO.
Ketoprofen Photodegradation Kinetics Promoted by TiO2
Authors: Paparo Rosanna , Viscovo Alessia , Trifuoggi Marco , Di Serio Martino , Russo Vincenzo
Published: September 2024

Ketoprofen is a non-biodegradable drug and is not removed by conventional treatments. The need to remove pharmaceutical compounds from water and wastewater has aroused considerable interest in advanced oxidation processes (AOP), whose effectiveness depends on the generation of reactive free radicals capable of oxidizing and decomposing numerous compounds. Heterogeneous photocatalysis is an efficient method if an active semiconductor is used. In this work, the photodegradation reaction of ketoprofen promoted by TiO2 was studied, analyzing the kinetics obtained by changing variables such as temperature, initial concentration, and quantity of photocatalyst. It was determined that the mechanism is of the Langmuir–Hinshelwood type and that the system is operating in the kinetic regime, while tests at different temperatures have shown that the adsorption of ketoprofen and byproducts are both exothermic. Experimental data were interpreted with reliable models that allow to retrieve quantitatively the kinetic and thermodynamic parameters.