bluuebunny/crossref_metadata_2025 huggingface dataset:
Pelatihan Pengolahan Data Untuk Analisis Regresi Menggunakan Software Statistical Product And Service Solution (SPSS)
Authors: Juharsah Juharsah , Madi Riski Amalia , Hartini Hartini , Hamid Wahyuniati , Yusuf Yusuf , Hastuti Hastuti
Published: March 2022

Kemampuan pengolahan data hasil penelitian sangat dibutuhkan untuk mempercepat penyelesaian penelitian dan pada akhirnya dapat mempercepat penyelesaian studi. Tujuan kegiatan pelatihan ini adalah untuk meningkatkan pengetahuan dan kemampuan peserta tentang; teori analisis regresi, pengolahan data menggunakan software SPSS, intepretasi output analisis regresi, dan meningkatkan kualitas penelitian. Kerangka pemecahan masalah kegiatan pelatihan ini dilakukan dalam beberapa  tahapan,  yaitu:  mengidentifikasi peserta pelatihan, mengetahui pengetahuan dan kemampuan para peserta, menyampaikan materi pelatihan, dan evaluasi hasil kegiatan. Berdasarkan hasil evaluasi diketahui bahwa adanya peningkatan kemampuan peserta dalam pengolahan data untuk analisis regresi menggunakan software SPSS.
Solusi Pembelajaran Anak Usia Dini Pada Masa Pandemi Covid-19
Authors: Koedoes Yuni Aryani , Abubakar St. Rahmaniar , Hijriani Hijriani , Anshari Nur Muh Nadzirin
Published: March 2022

Saat ini pandemi covid-19 melanda dunia dan Indonesia, banyak aspek yang berdampak khusunya di dunia pendidikan dan terkhusus lagi di pembelajaran anak usia dini,  sekolah PAUD saat ini banyak terkendala dengan model dan sistem pembelajarn pada masa pandemic yang tidak memungkinkan pertemuan tatap muka langsung sehingga diperlukan solusi yang tepat untuk pembelajaran anak usia dini. Salah satu solusi adalah dengan melatih guru-guru PAUD dalam pembuatan dan pengembangan media pembelajaran interaktif berbasis multimedia mencakup asepek video, animasi, gambar dan audio serta pembuatan aplikasi games interaktif yang dikhususkan untuk pendidikan anak usia dini, penerapan multimedia ditujukan untuk memudahkan murid PAUD dalam memahami materi pelajaran karena memuat informasi gambar, video dan audio serta dengan muatan materi yang positif sesuai kebutuhan kurikulum PAUD. Metode yang digunakan adalah dengan metode pelatihan dan pendampingan, sehingga guru dapat melakukan pembuatan media pembelajaran serta akan dilanjutkan dengan pendampingan, untuk pelatihan menggunakan metode ADDIE Analysis, Design, Development, Implementation, and Evaluation), dimana model ini sangat baik digunakan dalam Instructional Design, khususnya dalam pembuatan media pembelajaran, Pada masa pandemi covid-19 ini kegiatan pengabdian masyarakat menyesuaikan dengan kondisi yaitu kami melaksanakan secara online dengan dikombinasikan pendampingan langsung dengan protokol kesehatan yang kegiatan, walau dilaksanakan secara online kegiatan dapat terlaksana dengan baik.Kata Kunci ; PAUD, Media, Pembelajaran, Multimedia, covid-19
Pembuatan Server Lokal dan Sistem Informasi Desa Berbasis Open Source Software sebagai Media Penyimpanan Bahan Ajar di Kecamatan Gu, Kabupaten Buton Tengah, Provinsi Sulawesi Tenggara
Authors: Ransi Natalis , Surimi La , Sajiah Adha Mashur , Nusantara Ambo Wonua
Published: March 2022

Salah satu dampak wabah COVID-19 adalah proses belajar mengajar dengan cara tatap muka di ruang kelas pada semua jenjang diliburkan. Akibatnya belajar dari rumah menjadi solusi. Pemerintah melalui Kementrian Pendidikan dan Kebudayaan memerintahkan untuk mengoptimalkan pembelajaran melalui konten TVRI. Selain itu, beberapa sekolah/perguruan tinggi juga menerapkan proses belajar mengajar melalui media internet. Proses ini terkendala masalah jaringan internet dan kuota data yang dimiliki oleh masyarakat lebih khusus peserta didik. Hal ini disebabkan oleh konten video umumnya memerlukan kecepatan internet dengan standar tertentu dan membutuhkan kuota yang tidak sedikit. Berdasarkan kondisi tersebut kami melaksanakan kegiatan pengabdian pembuatan server lokal dan sistem informasi desa. Sistem yang dihasilkan menjadi tempat menyimpan konten pembelajaran yang dapat diakases oleh masyarakat tanpa perlu terhubung dengan internet, cukup melalui jaringan lokal yang dibangun. Konten berupa bahan ajar diperoleh dari hasil karya mahasiswa KKN berupa video pembelajaran. Tema pada setiap video sesuai dengan program studi mahasiswa KKN. Selain itu, rekaman bincang santai bersama kepala desa, ahli, praktisi selama kegiatan ini berlangsung menjadi konten pada sistem ini.Kata Kunci: server lokal, sistem informasi desa, bahan ajarPENDAHULUAN
Pelatihan Pembuatan Sabun Antiseptik Cair untuk Meminimalisasi Penularan Covid Melalui Gerakan Cuci Tangan
Authors: Kadidae La Ode , Kadir Laode Abdul , Nugrawaty Andi Laila
Published: March 2022

Sambuli village lies along Nambo beach of Nambo district, City of Kendari Southeast Sulawesi province. This village is mainly occupied by fishermen and farmers. Nowadays, It is every citizen’s responsibility to remind each other about the danger of Covid-19 and the importance of how to limit the spread of the disease. Considering this issue, Institute of Research and Community Service Halu Oleo University arranged a team consisting of 3 academic-staff; two of them from Chemistry study program and one from Biotechnology study program, and 3 students. All the three students were from Chemistry Department.  The aims of the team sent to the village were first to educate people of Sambuli about how to minimize the spread of Covid-19 disease and second is to train people of Sambuli to make liquid antiseptic soap. The soap would be used as an economical and practical substance for hand washing. The method used in this community service included public talking (mini seminar) and demonstration. During this community service, villagers got involved actively in discussion and participating actively during demonstration. They and their leaders were equally very enthusiastic and supportive. And in turn, the villagers with the assistance of students and academic staff of Halu Oleo University eventually managed to make liquid antiseptic soap by them shelves.
Edukasi Pencegahan Penyebaran Covid-19 Pada Masyarakat Desa Panggulawu Kabupaten Konawe Utara Di Era New Normal
Authors: Ismail Darwin , Kadir Laode A. , Wahab Wahab
Published: March 2022

Panggulawu village lies along Panggulawu beach of Sawa, northern konawe district, Southeast Sulawesi province. This village is mainly occupied by civil servants (PNS), fishermen and farmers. Nowadays, It is every citizen’s responsibility to remind each other about the danger of Covid-19 and the importance of how to limit the spread of the disease. Considering this issue, Institute of Research and Community Service Halu Oleo University arranged a team consisting of 3 academic-staff; two of them from Chemistry study program and one from Mining study program, and 3 students. All the three students were from Chemistry Department.  The aims of the team sent to the village is to educate people of Panggulawu about how to minimize the spread of Covid-19 disease. The method used in this community service included is public talking (mini seminar). During this community service, villagers got involved actively in discussion and participating actively during demonstration. They and their leaders were equally very enthusiastic and supportive.
Pendampingan Teknis Aplikasi Teknologi Mesin Tetas di Kelurahan Labibia Kecamatan Mandonga Kota Kendari
Authors: Saili Takdir , Nafiu La Ode , Arsad Sani La Ode , Libriani Restu , Abadi Musram , Sulfitrana Andini , Surahmanto Surahmanto , Dewi S Rachmita
Published: March 2022

Peternak ayam kampung di Kelurahan Labibia Kecamatan Mandonga Kota Kendari belum ada sentuhan teknologi tepatguna dalam  meningkatkan produktivitasnya. Peternak masih menggunakan sistem pengeraman alami, sehingga peningkatan produksi ayam menjadi rendah, disisi lainnya adalah produksi telur ayam kampung hanya mencapai 40 butir pertahun yang disebabkan induk ayam masih membutuhkan waktu untuk mengasuh anaknya setelah telur ditetaskan secara alami. Oleh karena itu, perlu dilakukan upaya penerapan teknologi melalui penetasan secara buatan (artificial incubation) dengan mesin tetas yang dapat memberikan manfaat dan memungkinkan tersedianya bibit ayam dalam jumlah yang banyak. Disisi lain penggunaan mesin tetas dapat menekan biaya pembelian DOC, sehingga dapat meningkatkan pendapatan bagi peternak ayam kampung. Pelaksanaan pengabdian Program Bina Desa Fakultas Peternakan UHO diharapkan dapat memberikan solusi atas permasalahan yang dihadapi oleh peternak, yaitu dengan memperkenalkan inovasi teknlogi budidaya ayam kampung  melalui pendampingan teknis aplikasi teknologi mesin tetas. Program Bina Desa Fakultas Peternakan UHO dilaksanakan di Kelurahan Labibia Kecamatan Mandonga Kota Kendari. Pelaksanaan program dilakukan dalam bentuk penyuluhan dan bimbingan teknis. Materi penyululuhan meliputi penguatan kelembagaan, konsep dasar inovasi teknologi mesin tetas sederhana dan ekonomis, metode pengoleksian telur tetas dan  metode pengoperasian alat mesin tetas. Sedangkan materi pada bimbingan teknis meliputi tehnik pembuatan mesin tetas, tehnik pengoleksian telur tetas dan tehnik pengoperasian alat mesin tetas. Berdasarkan kegiatan yang telah dilaksanakan, hasil yang telah dicapai pada Program Bina Desa di Kelurahan Labibia Kecamatan Mandonga Kota Kendari  dengan penerapan inovasi teknologi sederhana melalui bimbingan teknis pembuatan mesin tetas dapat meningkatakan pengetahuan dan keterampilan peternak dalam pembuatan mesin tetas pada kelompok ternak, serta dapat  menekan biaya pembelian DOC sehingga dapat meningkatkan pendapatan peternak
Pengembangan Turqiat.Id Sebagai Platform Iklan Digital Lokal Dalam Membantu Promosi Produk dan Jasa Usaha Mikro, Kecil Dan Menengah (UMKM) Sulawesi Tenggara di Masa Pandemik Covid-19
Authors: Sajiah Adha Mashur , Golok Jaya La Ode Muhammad , Ransi Natalis , Purnama Ika Purwanti Ningrum , Nangi Jumadil
Published: March 2022

Ikatan Pengusaha Muslimah Indonesia (IPEMI) adalah salah satu wadah bagi pengusaha-pengusahaUsaha Mikro, Kecil dan Menengah (UMKM) yang berfokus pada para pengusaha muslimah. IPEMIbertujuan untuk membantu pengusaha muslimah dalam mengembangkan usahanya. IPEMI memilikicabang di seluruh provinsi Indonesia salah satunya Sulawesi Tenggara. Permasalahan padakelompok usaha tentunya ada berbagai macam dan di segala level proses (produksi, pengiriman,pemasaran, purna jual ataupun pengendalian mutu). Dalam aspek pemasaran pengusaha perlumelakukan promosi agar produk/jasa mereka dikenal khalayak luas. Usaha promosi dan pemasarandi masa pandemik Covid-19 lebih sulit karena terdapat kewajiban protokol kesehatan ketikamelakukan aktivitas apalagi adanya social distancing. Konsumen lebih banyak di rumah danmemanfaatkan perangkat digital yang terhubung dengan internet. Oleh karena itu pengusaha UMKMperlu melakukan shifting pemasaran ke arah pemasaran digital. Melihat permasalahan tersebut makadalam kegiatan program kemitraan masyasrakat ini bertujuan untuk mengembangkan platform iklandigital khusus daerah Kota Kendari dan Sulawesi Tenggara. Platform ini menjadi tempat parapengusaha mengiklankan produk dan jasanya secara gratis. Proses pengembangan platform inimengikuti proses siklus hidup pengembangan rekayasa perangkat lunak (Software Development LifeCycle) dengan mempertimbangkan kebutuhan dari pengusaha yang ingin produknya dipromosikan.Setelah platform yang diberi nama Turqiat.id dikembangkan dilakukan pelatihan bagi pengusahabagaimana mengoperasikan dan memanfaatkan platform tersebut. Selain itu pengusaha dibekalidengan pengenalan pemanfaatan tools dan media pendukung digital serta desain iklan digital. Hasildari program kemitraan masyrakat ini para pengusaha UMKM telah dapat menggunakan platformTurqiat.id sebagai media iklan digital dan dapat mendesain iklan mereka sendiri.Kata Kunci: IPEMI, UMKM, Platform Iklan Digital, Pemasaran Digital, Covid-19
Pelatihan Pemurnian Minyak Nilam Hasil Suling Konvensional Masyarakat Di Desa Awalo Kecamatan Benua Konawe Selatan Melalui Teknik Filtrasi Sederhana
Authors: Alimin Alimin , Agusu La , Azis Thamrin , Mashuni Mashuni , Halimahtussadiyah Halimahtussadiyah , Darusman La Ode Muhammad
Published: March 2022

Penyulingan minyak nilam di desa Awalo masih menggunakan teknik penyulingan konvensional sehingga minyak nilam (patchouli oil) yang dihasilkan dari penyulingan tersebut masih berwarna cokelat tua yang mengindikasikan bahwa minyak nilam yang dihasilkan dari penyulingan tersebut belum memenuhi baku mutu minyak nilam sesuai SNI 06-2385-2006. Hasil penelitian menunjukkan bahwa selain patchouli alcohol yang merupakan komponen penyusun utama minyak nilam, kontaminan seperti ion logam Fe juga dijumpai dalam minyak nilam. Beberapa peneliti melaporkan bahwa sumber utama ion logam tersebut berasal dari wadah penyulingan konvensional pada saat proses penyulingan. Kehadiran ion logam seperti Fe dalam minyak nilam akan menyebabkan pembentukan ion kelat yang dapat mengakibatkan warna minyak nilam menjadi cokelat tua. Solusi dari masalah ini dapat dilakukan melalui teknik filtrasi (penyaringan) sederhana menggunakan prinsip adsorpsi berbasis silika, arang aktif dan batuan mangan alam. Minyak nilam hasil penyulingan konvensional di desa Awalo di treatment lebih lanjut dengan peralatan filtrasi yang direkomendasikan oleh tim pengabdi. Teknik filtrasi sederhana berbasis silika, arang aktif dan mangan terhadap minyak nilam sehingga mitra memiliki keterampilan softskill dan hardskill dalam meningkatkan kualitas minyak nilam. Untuk merealisasikan kegiatan ini maka pelaksanaan PKMI dibagi dalam dua kegiatan utama yaitu kegiatan laboratorium dan kegiatan lapangan.
Intensifikasi Usaha Ayam Kampung Melalui Teknologi Pakan dan Inseminasi Buatan untuk Meningkatkan Produktivitas Ternak
Authors: Saili Takdir , Badaruddin Rusli , Syamsuddin Syamsuddin , Salido Wa Laili , Isnaeni Purnaning Dhian
Published: March 2022

Melimpahnya sumber pakan, baik berupa jagung, ampas tahu, dan kulit ari kedelai di Desa Alebo, Kecamatan Konda, Konawe Selatan, dapat dimanfaatkan untuk meningkatkan produksi ayam kampung sehingga dapat diperoleh hasil yang optimal. Usaha produksi ayam kampung yang dijalankan masyarakat Desa Alebo masih terbatas untuk tujuan produksi daging dan sangat memungkinkan untuk dikembangkan menjadi usaha produksi ayam kampung dengan tujuan menghasilkan telur baik untuk telur konsumsi maupun untuk telur bibit. Program PKM ini bertujuan untuk meningkatkan pengetahuan dan keterampilan peternak dalam memanfaatkan sumber pakan yang ada di sekitar peternak untuk membuat pakan ayam kampung yang berkualitas dengan harga murah, dalam mengaplikasikan teknologi reproduksi, serta pencegahan penyakit untuk mendukung produktivitas ayam kampung. Metode yang digunakan adalah metode kegiatan non fisik meliputi diskusi dan praktek bersama, serta metode kegiatan fisik berupa pelatihan pembuatan pakan, inseminasi buatan, dan pengoperasian mesin tetas.
Bimbingan Teknis Penyusunan Master Plan Penataan Permukiman Desa Mata Wawatu Kecamatan Moramo Utara Kabupaten Konawe Selatan
Authors: Kadir Ishak , Ma’ruf Annas , Tahir M. Arzal , Hasan La Ode Amrul , Aulia Weko Indira Romanti
Published: March 2022

Penataan permukiman Desa Mata Wawatu belum berjalan secara maksimal dan terarah karena belum memiliki pola dan arah kebijakan pengembangan wilayah secara berkelanjutan. Permasalahan umum yang dihadapi masyarakat antara lain: [1] potensi Sumber Daya Alam belum tertata dengan baik karena belum memiliki arah kebijakan pembangunan yang jelas, [2] pengetahuan masyarakat dalam penataan lingkungan permukiman masih kurang. Metode pelaksanaan kegiatan yaitu: [1] model Participatory Rural Appraisal yang menekankan keterlibatan masyarakat dalam keseluruhan kegiatan mulai dari perencanaan, pelaksanaan dan evaluasi program kegiatan, [2] model Participatoty Tecnology Development yang memanfaatkan teknologi tepat guna yang sesuai standar berbasis pada ilmu pengetahuan dan kearifan lokal, [3] model Community Development yaitu pendekatan yang melibatkan masyarakat secara langsung, dan [4] Edukatif. Langkah-langkah yang ditempuh dalam pengabdian ini adalah tahap persiapan, tahap pembekalan, dan pelaksanaan. Hasil kegiatan program kemitraan masyarakat menunjukkan bahwa pengetahuan dan keterampilan warga masyarakat Desa Mata Wawatu mengalami peningkatan setelah mengikuti bimbingan teknis. Masyarakat telah mampu mengidentifikasi potensi dan permasalahan desa serta analisisnya secara partisipatif.
Upaya Pencegahan Penyebaran Covid-19melalui Penyuluhan Serta Pemasangan Media Informasi Pada Area Publik Dan Rumah Warga Di Desa Wawouru Dan Desa Mekarsari Kecamatan Palanggakabupaten Konawe Selatan Provinsi Sulawesi Tenggara Tahun 2021
Authors: Majid H. Ruslan , Zainuddin Asnia , Hartoyo Agnes Mersatika
Published: March 2022

Mitra yang terlibat dalam kegiatan ini adalah warga yang tinggal di Desa Wawouru dan DesaMekarsari Kecamatan Palangga Kabupaten Konawe Selatan Provinsi Sulawesi Tenggara. Dari hasil observasi dan wawancara dengan Kepala Desa hampir 80% warga di Desa Wawouru dan 90% warga Desa Mekarsari tidak menggunakan masker, beberapa kegiatan yang menimbulkan orang berkerumun tanpa menjaga jarak, serta tidak memahami gejala dan siklus penyebaran Covid-19. Solusi yang ditawarkan memberikan penyuluhan tentang gejala, siklus penyebaran, dampak serta upaya pencegahan Covid-19 guna meningkatkan pengetahuan dan kesadaran terhadap kesehatan ditingkat personal, keluarga dan masyarakat. Penyuluhan dilakukan bekerjasama dengan tenaga kesehatan dari Puskesmas setempat dan Satgas Covid-19. Langkah selanjutnya adalah mengingatkan warga akan bahaya Covid-19 dengan memasang media informasi seperti baliho di area publik dan beberapa titik lokasi yang letaknya strategis (sering dilewati warga), serta mengingatkan warga agar selalu mematuhi protokol kesehatan dengan menempel poster/stiker di masing-masing rumah warga. Penempelan poster pada masing-masing rumah warga juga diharapkan dapat membantu orang tua untuk menanamkan kewaspadaan terhadap pencegahan penularan Covid-19 kepada anak sejak dini.
EVALUASI PEMANFAATAN DANA ALOKASI KHUSUS DALAM MENUNJANG PEMBANGUNAN PARIWISATA DI KABUPATEN KONAWE SELATAN
Authors: Irfan Irfan IRFANTRI
Published: March 2022

Penelitian ini bertujuan Untuk mengetahui evaluasi pemanfaatan dana alokasi khusus pada dinas pariwsata Kabupaten Konawe Selatan dan untuk mengetahui pembangunan pariwisata pada dinas pariwisata Kabupaten Konawe Selatan. Penelitian ini dilakukan dengan pendekatan kualitatif dengan metode deskripsif. Informan dalam penelitian ini berjumlah 9 (Sembilan) orang yang ditentukan dengan metode purposive sampling, teknik pengumpulan data dilakukan dengan cara wawancara mendalam, observasi dan studi dokumentasi. Teknik analisis data dalam penelitian ini yaitu reduksi data, penyajian data dan penarikan kesimpulan/verifikasi. Hasil penelitian menunjukan bahwa pemanfaatan dana alokasi khusus pada dinas pariwisata kabupaten konawe selatan telah memenuhi sebagian besar unsur-unsur yang telah ditentukan walaupun ada sebagian besar unsur yang belum terpenuhi secara maksimal yakni pemerataan karena tidak semua masyarakat luar objek wisata bisa merasakan manfaat maksimal oleh pengunjung wisata terkecuali jumlah pengunjung wisata yang begitu besar. Begitu pula pembangunan pariwisata yang ada di konawe selatan mengalami banyak perubahan positif dari sarana dan prasarananya dengan anggaran dana alokasi khusus sehingga ketiga unsur pembangunan pariwisata dapat terpenuhi yaitu daya tarik wisata, aksesbilitas, dan amenitas, walaupun salah satu indikator dari unsur amenitas belum terpenuhi secara maksimal yakni sarana transportasi umum. Kata Kunci : Dana alokasi khusus, daya tarik wisata, aksesbilitas dan amenitas.
Sulfur-Mediated Polycarbonate Polyurethane for Potential Application of Blood-Contacting Materials
Authors: Li Peichuang , Cai Wanhao , Li Xin , Zhang Hong , Zhao Yuancong , Wang Jin
Published: March 2022

In this study, a sulfur-mediated polycarbonate polyurethane (PCU-SS) is developed by mimicking the catalyzing ability of glutathione peroxidase (GPx) on nitric oxide (NO) in the human body. The PCU-SS is endowed with the capability to produce NO based on disulfide bonds, which could strongly improve the biocompatibility of the materials. The characterization results indicate that PCU-SS could not only decrease the adhesion of platelets but also enhance the capability of anti-thrombus. Moreover, it is shown that PCU-SS has a good compatibility with endothelial cells (ECs), while has a marked inhibition capacity of the proliferation of smooth muscle cells (SMCs) and macrophages (MA). Meanwhile, the result of animal implantation experiments further demonstrates the good abilities of PCU-SS on anti-inflammation, anti-thrombus, and anti-hyperplasia. Our results offer a novel strategy for the modification of blood-contacting materials based on disulfide bonds. It is expected that the PCU-SS could shed new light on biocompatibility improvement of cardiovascular stents.
Fatty Acid Supplementation During in vitro Embryo Production Determines Cryosurvival Characteristics of Bovine Blastocysts
Authors: Aardema H. , Bertijn I. , van Tol HTA. , Rijneveld A. , Vernooij JCM. , Gadella B. M. , Vos PLAM
Published: March 2022

In vitro production (IVP) embryos have a reduced quality and poor cryotolerance in comparison to in vivo embryos. This study investigated whether free fatty acid (FFA) conditions, fatty acid free (FAF)- synthetic oviduct fluid (SOF) without or with 25 μM of saturated stearic (C18:0) or unsaturated oleic (C18:1) acid during the first 5 IVP days, relate to quality and cryosurvival of day 8 blastocysts. Apart from the blastocyst scores, both 1) number and size of lipid droplets of fresh blastocysts and 2) total number and apoptotic and necrotic cells, before and after freezing-thawing, were scored by confocal microscopy. Blastocyst rates were significantly lower in the FAF SOF condition in comparison to other groups. Interestingly, blastocysts originating from the C18:1 group, with a significantly higher lipid content, and blastocysts from the FAF SOF group demonstrated a high cryosurvival rate (70.1 and 67.4%, respectively) comparable with in vivo blastocysts (68%), in contrast to the poor cryosurvival of C18:0 exposed embryos (17.6%). In all freeze-thawed embryos the average amount of apoptotic and necrotic cells increased albeit that the C18:0 condition rates were higher (43.2%) when compared to C18:1 (26.0%) and FAF SOF conditions (26.5%). The current data show that FFA administered during early embryonic development significantly affect the cryotolerance of blastocysts.
The Emerging Roles of LINC00665 in Human Cancers
Authors: Zhu Jing , Zhang Yirao , Chen Xuyu , Bian Yibo , Li Juan , Wang Keming
Published: March 2022

Long non-coding RNAs (lncRNAs) are non-coding RNAs that have more than 200 nucleotides and can participate in the regulation of gene expression in various ways. An increasing number of studies have shown that the dysregulated expression of lncRNAs is related to the occurrence and progression of human cancers. LINC00665 is a novel lncRNA, which is abnormally expressed in various human cancers, such as lung cancer, breast cancer, prostate cancer, and glioma. LINC00665 functions in many biological processes of tumor cells, such as cell proliferation, migration, invasion, angiogenesis, and metabolism, and is related to the clinicopathological characteristics of cancer patients. LINC00665 can play biological functions as a ceRNA, directly binding and interacting with proteins, and as an upstream molecule regulating multiple signaling pathways. In this review, we comprehensively summarize the expression level, function, and molecular mechanisms of LINC00665 in different human cancers and emphasize that LINC00665 is a promising new diagnostic, prognostic biomarker, and therapeutic target.
The Roles of Junctional Adhesion Molecules (JAMs) in Cell Migration
Authors: Wang Junqi , Liu Han
Published: March 2022

The review briefly summarizes the role of the family of adhesion molecules, JAMs (junctional adhesion molecules), in various cell migration, covering germ cells, epithelial cells, endothelial cells, several leukocytes, and different cancer cells. These functions affect multiple diseases, including reproductive diseases, inflammation-related diseases, cardiovascular diseases, and cancers. JAMs bind to both similar and dissimilar proteins and take both similar and dissimilar effects on different cells. Concluding relevant results provides a reference to further research.
Differential Effects of Reperfusion on Cardiac Mitochondrial Subpopulations in a Preclinical Porcine Model of Acute Myocardial Infarction
Authors: Chandra Shekar Kadambari , Yannopoulos Demetris , Kosmopoulos Marinos , Riess Matthias L.
Published: March 2022

Acute myocardial infarction (AMI) leads to localized cardiac ischemia and can be fatal if untreated. Despite being treatable, the threat of ischemia-reperfusion (IR) injury remains high. Mitochondria are central to both propagation and mitigation of IR injury, and cardiac mitochondria are categorized into two major subtypes—subsarcolemmal and interfibrillar mitochondria (SSM and IFM, respectively). We hypothesized that, in our pre-clinical porcine model of AMI, SSM and IFM are differentially affected by reperfusion. AMI was induced in female pigs by balloon occlusion of the left anterior descending artery for 45 min, followed by 4 h of reperfusion. At the end of reperfusion, animals were euthanized. Cardiac SSM and IFM from the affected ischemic area and a nearby non-ischemic area were isolated to compare mitochondrial function using substrates targeting mitochondrial electron transport chain complexes I and II. Despite detecting overall significant differences in mitochondrial function including yield, mitochondrial S3 and S4 respirations, and calcium retention, consistent individual functional differences in the two mitochondrial subpopulations were not observed, both between the two mitochondrial subtypes, as well as between the ischemic and non-ischemic tissue. Nonetheless, this study describes the mitochondrial subtype response within the initial few hours of reperfusion in a clinically relevant model of AMI, which provides valuable information needed to develop novel mitochondrially targeted therapies for AMI.
Layered Double Hydroxides Containing Rare Earth Cations: Synthesis and Applications
Authors: Seliverstov Evgeniy S. , Golovin Sergei N. , Lebedeva Olga E.
Published: March 2022

In this mini-review, we describe the currently available literature concerning synthesis and applications of layered double hydroxides (LDHs) containing rare earth cations (RE-LDHs), focusing on the catalytic activity of those compounds. The lack of studies of some rare earth elements (REE) and the insufficient knowledge of their catalytic activity in the structure of LDHs indicate the need for further research.
Tungsten Promoted Ni/Al2O3 as a Noble-Metal-Free Catalyst for the Conversion of 5-Hydroxymethylfurfural to 1-Hydroxy-2,5-Hexanedione
Authors: Duan Ying , Wang Rui , Liu Qihang , Qin Xuya , Li Zuhuan
Published: March 2022

The conversion of 5-hydroxymethylfurfural (HMF) to 1-hydroxy-2,5-hexanedione (HHD) represented a typical route for high-value utilization of biomass. However, this reaction was often catalyzed by the noble metal catalyst. In this manuscript, W promoted Ni/Al2O3 was prepared as a noble-metal-free catalyst for this transformation. The catalysts were characterized by XRD, XPS, NH3-TPD, TEM, and EDS-mapping to study the influence of the introduction of W. There was an interaction between Ni and W, and strong acid sites were introduced by the addition of W. The W promoted Ni/Al2O3 showed good selectivity to HHD when used as a catalyst for the hydrogenation of HMF in water. The influences of the content of W, temperature, H2 pressure, reaction time, and acetic acid (AcOH) were studied. NiWOx/Al2O3-0.5 (mole ratio of W:Ni = 0.5) was found to be the most suitable catalyst. The high selectivity to HHD was ascribed to the acid sites introduced by W. This was proved by the fact that the selectivity to HHD was increased a lot when AcOH was added just using Ni/Al2O3 as catalysts. 59% yield of HHD was achieved on NiWOx/Al2O3-0.5 at 393 K, 4 MPa H2 reacting for 6 h, which was comparable to the noble metal catalyst, showing the potential application in the production of HHD from HMF.
Rapid and Accurate Differentiation of Mycobacteroides abscessus Complex Species by Liquid Chromatography-Ultra-High-Resolution Orbitrap™ Mass Spectrometry
Authors: Bajaj Amol O. , Slechta E. Susan , Barker Adam P.
Published: March 2022

In this study, a Liquid Chromatography-Mass Spectrometry (LC-MS) method for the identification of clinically relevant Mycobacteroides abscessus (Mabs) complex organisms is tested using a set of microbial Type strains. This methodology is based on profiling proteins derived from Mycobacteroides abscessus complex isolates. These protein profiles are then used as markers of species differentiation. To test the resolving power, speed, and accuracy of this assay four ATCC type strains and 32 recent clinical isolates of closely related Mabs species collected at ARUP laboratories (10 clinical isolate strains of M. abscessus subsp. abscessus, 10 M. abscessus subsp. massiliense, 2 M. abscessus subsp. bolletii and 10 M. chelonae) were subjected to this approach. Using multiple deconvolution algorithms, we identified hundreds of individual proteins, with subpopulations of these used as species-specific markers. This assay identified 150, 130, 140 and 110 proteoforms with isocratic elution and 230, 180, 200 and 190 proteoforms with gradient elution for M. abscessus (ATCC 19977), M. massiliense (DSM 45103), M. bolletii (DSM 45149) and M. chelonae (ATCC 35752) respectively. Taxonomic species were identified correctly down to the species level with 100% accuracy. The ability to differentiate Mycobacteroides abscessus complex at sub-species level can in-turn be helpful for patient management. Data analysis showed ~7-17 proteoforms potentially able to differentiate between subspecies. Here, we present a proof-of-principle study employing a rapid mass spectrometry-based method to identify the clinically most common species within the Mabs species complex.
Intestinal Flora Composition Determines Microglia Activation and Improves Epileptic Episode Progress
Authors: Ding Xiaomi , Zhou Jing , Zhao Li , Chen Mingyue , Wang Shenglin , Zhang Ming , Zhang Xiaodong , Jiang Guohui
Published: March 2022

In response to environmental stimuli, immune memory mediates the plasticity of myeloid cells. Immune training and immune tolerance are two aspects of plasticity. Microglia that are immunologically trained or immunologically tolerant are endowed with a tendency to differentiate into alternative dominant phenotypes (M1/M2). Male C57BL/6 mice (immune-training group, immune-tolerant group, and control group) were used to establish the kainic acid epilepsy model. The seizure grade, duration, latency, hippocampal potential, and energy density were used to evaluate seizures, and the changes in the polarization of microglia were detected by western blot. 16S rDNA sequencing showed that the abundance of Ruminococcus in the immune-tolerant group was the dominant flora. Our research connections Intestinal microorganisms, brain immune status, and epilepsy behavior together. Pro-inflammatory M1 phenotype and anti-inflammatory M2 phenotype mediate and enhance and suppress subsequent inflammation, respectively. We conclude that intestinal microorganisms influence the occurrence and development of epilepsy by regulating the polarization of microglia.
A Communication Complex Approach to Autism Awareness Training Within First Response Systems in Indiana
Authors: Rascon Natasha A.
Published: March 2022

Providing Autism and communication training to facilitate better interactions in emergency situations between first responders and individuals with Autism is a critical need. Although trainings exist for first responders, “typical training already being provided on the job is not sufficient” (IBCCES). In addition, while resources—such as YouTube videos—are available, the process for viewing, discussing, and debriefing over the material along with the development of more effective conversational practices is an unexplored space. This case study describes the use of the Communication Complex (CC) perspective for training Volunteer Firefighters in Indiana, emphasizing the development of knowledge and skills necessary for effectively managing this often challenging context. It extends the body of CC research that relates to first responders and other health care providers. An elaboration of the theoretical framing is provided, followed by a brief introduction to Autism and a discussion of safety concerns in the community. Finally, a detailed analysis of the case study is presented. Reports from the participants indicate that Autism awareness training through a CC approach can enable more desirable interaction patterns in emergency situations involving first responders and those with Autism. The findings suggest that the CC approach is effective for enhancing understanding, which can positively impact future scenarios involving individuals with Autism and their families and increase feelings of safety.
Determination of Agrin and Related Proteins Levels as a Function of Age in Human Hearts
Authors: Skeffington Katie L. , Jones Ffion P. , Suleiman M. Saadeh , Caputo Massimo , Brancaccio Andrea , Bigotti Maria Giulia
Published: March 2022

Background:

Mature cardiomyocytes are unable to proliferate, preventing the injured adult heart from repairing itself. Studies in rodents have suggested that the extracellular matrix protein agrin promotes cardiomyocyte proliferation in the developing heart and that agrin expression is downregulated shortly after birth, resulting in the cessation of proliferation. Agrin based therapies have proven successful at inducing repair in animal models of cardiac injury, however whether similar pathways exist in the human heart is unknown.

Methods

Right ventricular (RV) biopsies were collected from 40 patients undergoing surgery for congenital heart disease and the expression of agrin and associated proteins was investigated.

Results

Agrin transcripts were found in all samples and their levels were significantly negatively correlated to age (p = 0.026), as were laminin transcripts (p = 0.023), whereas no such correlation was found for the other proteins analyzed. No significant correlations for any of the proteins were found when grouping patients by their gender or pathology. Immunohistochemistry and western blots to detect and localize agrin and the other proteins under analysis in RV tissue, confirmed their presence in patients of all ages.

Conclusions

We show that agrin is progressively downregulated with age in human RV tissue but not as dramatically as has been demonstrated in mice; highlighting both similarities and differences to findings in rodents. Our results lay the groundwork for future studies exploring the potential of agrin-based therapies in the repair of damaged human hearts.
A Risk Score to Diagnose Cardiac Involvement and Provide Prognosis Information in Patients at Risk of Cardiac Light-Chain Amyloidosis
Authors: Wu Yan , Pu Cailing , Zhu Wenchao , He Chengbin , Fei Jingle , Hu Hongjie
Published: March 2022

Background

Cardiac light-chain amyloidosis (AL CA) portends poor prognosis. Contrast cardiac magnetic resonance (CMR) with late gadolinium enhancement (LGE) imaging is an important tool in recognizing AL CA. But contraindications to contrast CMR would significantly restrict its clinical application value. Our study aims to construct a convenient risk score to help identify cardiac involvement in patients at risk of AL CA. Moreover, we also investigate whether this risk score could provide prognosis information.

Materials and Methods

Sixty-three patients at risk of AL CA were retrospectively included in our study. Basic clinical characters, lab results, 12-lead electrocardiogram data, and cardiac magnetic resonance image data were collected. AL CA was diagnosed according to typical CA LGE pattern. Logistic analysis was used to figure out predictive parameters of AL CA and their β coefficients, further constructing the risk score. Receiver operating characteristics (ROC) curve was used to find the cut-off point best distinguishing AL CA+ from AL CA–patients. Bootstrapping was used for internal validation. All patients were divided into high-risk and low-risk group according to the diagnostic cut-off point, and followed up for survival information. Kaplan-Meier plots and log-rank test were performed to analyze if this score had prognostic value.

Results

The risk score finally consisted of 4 parameters: pericardial effusion (PE) (1 point), low electrocardiographic QRS voltages (LQRSV) (1 point), CMR-derived impaired global radial strain (GRS) (<15.14%) (1 point) and increased left ventricular maximum wall thickness (LVMWT) (>13 mm) (2 points). Total score ranged from 0 to 5 points. A cut-off point of 1.5 showed highest accuracy in diagnosing AL CA with an AUC of 0.961 (95% CI: 0.924–0.997, sensitivity: 90.6%, specificity: 83.9%). Kaplan-Meier plots and log-rank test showed that the high-risk group had significantly poor overall survival rates.

Conclusion

In patients at risk of AL CA, a risk score incorporating the presence of PE, LQRSV, and CMR-derived impaired GRS and increased LVMWT is predictive of a diagnosis of AL CA by LGE criteria. This risk score may be helpful especially when contrast CMR is not available or contraindicated, and further studies should be considered to validate this score.
Impact of Valve Size on Paravalvular Leak and Valve Hemodynamics in Patients With Borderline Size Aortic Valve Annulus
Authors: Talmor-Barkan Yeela , Kornowski Ran , Bar Noam , Ben-Shoshan Jeremy , Vaknin-Assa Hanna , Hamdan Ashraf , Kruchin Boris , Barbash Israel M. , Danenberg Haim , Perlman Gidon Y. , Konigstein Maayan , Finkelstein Ariel , Steinvil Arie , Merdler Ilan , Segev Amit , Barsheshet Alon , Codner Pablo
Published: March 2022

Background

Transcatheter heart valve (THV) selection for transcatheter aortic valve implantation (TAVI) is crucial to achieve procedural success. Borderline aortic annulus size (BAAS), which allows a choice between two consecutive valve sizes, is a common challenge during device selection. In the present study, we evaluated TAVI outcomes in patients with BAAS according to THV size selection.

Methods

We performed a retrospective study including patients with severe aortic stenosis (AS) and BAAS, measured by multi-detector computed tomography (MDCT), undergoing TAVI with self-expandable (SE) or balloon-expandable (BE) THV from the Israeli multi-center TAVI registry. The aim was to evaluate outcomes of TAVI, mainly paravalvular leak (PVL) and valve hemodynamics, in patients with BAAS (based on MDCT) according to THV sizing selection in between 2 valve sizes. In addition, to investigate the benefit of shifting between different THV types (BE and SE) to avoid valve size selection in BAAS.

Results

Out of 2,352 patients with MDCT measurements, 598 patients with BAAS as defined for at least one THV type were included in the study. In BAAS patients treated with SE-THV, larger THV selection was associated with lower rate of PVL, compared to smaller THV (45.3 vs. 64.5%; pv = 0.0038). Regarding BE-THV, larger valve selection was associated with lower post-procedural transvalvular gradients compared to smaller THV (mean gradient: 9.9 ± 3.7 vs. 12.5 ± 7.2 mmHg; p = 0.019). Of note, rates of mortality, left bundle branch block, permanent pacemaker implantation, stroke, annular rupture, and/or coronary occlusion did not differ between groups.

Conclusion

BAAS is common among patients undergoing TAVI. Selection of a larger THV in these patients is associated with lower rates of PVL and optimized THV hemodynamics with no effect on procedural complications. Additionally, shift from borderline THV to non-borderline THV modified both THV hemodynamics and post-dilatation rates.
Long COVID-19 and Postural Orthostatic Tachycardia Syndrome- Is Dysautonomia to Be Blamed?
Authors: Chadda Karan R. , Blakey Ellen E. , Huang Christopher L. -H. , Jeevaratnam Kamalan
Published: March 2022

While the increased arrhythmic tendency during acute COVID-19 infection is recognised, the long-term cardiac electrophysiological complications are less well known. There are a high number of patients reporting ongoing symptoms post-infection, termed long COVID. A recent hypothesis is that long COVID symptoms could be attributed to dysautonomia, defined as malfunction of the autonomic nervous system (ANS). The most prevalent cardiovascular dysautonomia amongst young people is postural orthostatic tachycardia syndrome (POTS). Numerous reports have described the development of POTS as part of long COVID. Possible underlying mechanisms, although not mutually exclusive or exhaustive, include hypovolaemia, neurotropism, inflammation and autoimmunity. Treatment options for POTS and other long COVID symptoms are currently limited. Future research studies should aim to elucidate the underlying mechanisms of dysautonomia to enable the development of targeted therapies. Furthermore, it is important to educate healthcare professionals to recognise complications and conditions arising from COVID-19, such as POTS, to allow prompt diagnosis and access to early treatment.
A Continuous 13.3-Ka Paleoseismic Record Constrains Major Earthquake Recurrence in the Longmen Shan Collision Zone
Authors: Shi Wei , Jiang Hanchao , Alsop G. Ian , Wu Guo
Published: March 2022

Thrust collision zones with low slip rates along the plate boundary are significant areas of stress accumulation and prone to develop more destructive earthquakes with longer recurrence intervals. Such regions are often classified as low seismic risk if they lack continuous records of large earthquakes, such as the eastern Tibetan Plateau before the 2008 Mw 7.9 Wenchuan earthquake. Here, we provide a continuous seismic record in the Longmen Shan thrust fault zone spanning 13,000 years based on detailed investigation of the soft-sediment deformation structures and seismites in the Lixian lacustrine sequence. The recurrence time of large earthquakes (M ≥ 8.1) is 1,200 years, which is significantly shorter than the previous estimate of 2,000–6,000 years. The Maoxian-Wenchuan fault is the main fault that triggered the deformation in the Lixian lacustrine sediments. In addition, earthquake recurrence in the warm period is more frequent than that in the cold period, which should arouse our attention for the seismic study of tectonically active regions.
Black Shale Paleo-Environmental Reconstructions: A Geochemical Case Study of Two Ordovician–Silurian Boundary Sections in Middle Yangtze Area, China
Authors: Yi Xuefei , Ji Xia , Huang Yunfei , Liu Zuosong , Meng Jianghui
Published: March 2022

Under the promotion of exploration and development for shale oil and gas, marine shale has become a hotspot of fine-grained sedimentary studies in China. Paleo-environment reconstruction has always been an important aim, especially for black shale. Based on a large number of samples collected from Wufeng and Longmaxi Formations of two field sections in western Hubei and eastern Chongqing, this study compares the vertical variations in total organic carbon content, quartz/clay mineral value, and trace element compositions of these two profiles to reflect the paleo-environment changes from the latest Ordovician to the earliest Silurian. The results show that during the later Ordovician period, when Wufeng Formation deposited, the study area experienced a water depth change from relative deep facies to shallow marine, and paleo-redox changed from oxygen rich to anoxic, and even euxinic, followed by the content of oxygen increase. When the Guanyinqiao Bed deposited, the basin suddenly became shallower, and the deposition environment changed to be oxygen enriched for high water energy, circulating oxygen enriched. In the earliest stage Silurian, when the Longmaxi Formation formed, and the paleo-water also suffered several changes of sea level, and the paleo-environment also changed from anoxic to oxic. These changes can correspond to the third-forth order sequences well.
Crustal Structure and Anisotropy in the Lower Yangtze Region and its Metallogenic Implications
Authors: Han Rubing , Yang Dinghui , Li Qiusheng , Chen Hao , Zhang Hongshuang , Ye Zhuo , Cheng Yongzhi , Fu Wei
Published: March 2022

In this study, we performed receiver function profiling and fitted harmonic functions to the arrival time variations of Pms phases to calculate the crustal seismic anisotropy with delay time and fast polarization direction, using broadband seismic data obtained from 55 temporary stations in two linear profiles and 39 stations in the Lower Yangtze and adjacent region. Moreover, we determined the crustal thickness and Poisson’s ratio using a novel H-κ-c stacking method. Our results revealed that the Middle-Lower Yangtze Metallogenic Belt and the north east section of the Qinzhou-Hangzhou Metallogenic Belt are characterized by Moho upliftment (<32 km), a relatively high Poisson’s ratio (>0.26), local lithospheric thinning (<70 km), and a pattern of deep faults that connect the crust and asthenosphere and serve as conduits for magma upwelling. The NE-SW fast polarization direction was consistent with the SKS splitting results, and the average delay time was 0.45 s. Moreover, underplating of deep magma and upwelling along the weak zone caused local Moho uplift and ductile shear of the lower crust, resulting in the directional arrangement of amphibole and other minerals, which may be the controlling mechanism for the crustal anisotropy in the study area. The variations in crustal structure and anisotropy characteristics indicated that in the context of the northeastern Paleo-Pacific plate subduction, the existence of weak lithospheric zones and the northeastern asthenospheric flow are important conditions for metal supernormal enrichment in the Lower Yangtze region.