bluuebunny/crossref_metadata_2025 huggingface dataset:
Teacher Trainees’ Well-Being—The Role of Personal Resources
Authors: Hohensee Elena , Weber Kira Elena
Published: July 2022

Teacher well-being is intrinsically associated with their personal resources, including health literacy and occupational self-regulation. However, there are few empirical findings on teacher trainees’ health literacy. Furthermore, occupational self-regulation has so far only been associated with indicators of occupational well-being. From a public health perspective, research on teacher trainees’ general well-being will benefit from taking both research aspects into account. In this study, we analysed data from 407 teacher trainees in Germany. Latent profile analysis confirmed the four occupational self-regulatory types (healthy-ambitious, unambitious, excessively ambitious, and resigned), which differed significantly on the health literacy dimensions self-regulation, self-control, self-perception, proactive approach to health, communication and cooperation, and dealing with health information. The health literacy dimensions of self-regulation and self-control were mainly related to occupational self-regulation. Independently of each other, the self-regulatory types and the health literacy dimensions of self-regulation, self-control, and proactive approach to health predicted teacher trainees’ general well-being. If both constructs are considered together, the health literacy dimensions explain more variance in teacher trainees’ general well-being than the self-regulatory types. Research and practical implications are discussed.
Development of a Tool for Measuring Ventilator-Associated Pneumonia Prevention Behaviors of Intensive Care Unit Nurses
Authors: Kwak Sungjung , Han Sujeong
Published: July 2022

Introduction: Ventilator-associated pneumonia (VAP) lengthens intensive care unit (ICU) stays and increases medical expenses and mortality risk. Aim: We aimed to develop and validate a tool for measuring VAP prevention behaviors among ICU nurses. Method: This is a methodological study that included a tool development step, based on the procedure suggested by DeVellis, and a tool verification step. Results: Through a literature review and focus interviews, 35 preliminary items were selected. After a content validity examination by experts and a pre-test, 30 items were chosen for this study. In the testing phase of the main survey, the final version tool was used on 452 ICU nurses to assess validity and reliability. From factor analysis, 7 factors and 17 items were selected. The factors included aspiration prevention, ventilator management, oral care, suction system management, subglottic suction, spontaneous awakening trials and spontaneous breathing trials, and standard precautions. The total determination coefficient was 71.6%. These factors were verified using convergent, discriminant, and concurrent validity tests. Internal consistency reliability was acceptable (Cronbach’s α = 0.80); thus, the VAP prevention behavior measurement tool was proven valid and reliable. Conclusions: This tool can be used with ICU nurses to measure behaviors associated with VAP prevention and, in turn, VAP prevention measures can be improved.
Online Behaviours during the COVID-19 Pandemic and Their Associations with Psychological Factors: An International Exploratory Study
Authors: Burkauskas Julius , Fineberg Naomi , Ioannidis Konstantinos , Chamberlain Samuel , Bowden-Jones Henrietta , Griskova-Bulanova Inga , Pranckeviciene Aiste , Dores Artemisa , Carvalho Irene , Barbosa Fernando , Simonato Pierluigi , De Luca Ilaria , Mooney Rosin , Gómez-Martínez Maria , Demetrovics Zsolt , Ábel Krisztina , Szabo Attila , Fujiwara Hironobu , Shibata Mami , Melero-Ventola Alejandra , Arroyo-Anlló Eva , Santos-Labrador Ricardo , Kobayashi Kei , Di Carlo Francesco , Monteiro Cristina , Martinotti Giovanni , Corazza Ornella
Published: July 2022

This cross-sectional study aimed to explore specific online behaviours and their association with a range of underlying psychological and other behavioural factors during the COVID-19 pandemic. Eight countries (Italy, Spain, the United Kingdom, Lithuania, Portugal, Japan, Hungary, and Brazil) participated in an international investigation involving 2223 participants (M = 33 years old; SD = 11), 70% of whom were females. Participants were surveyed for specific type of Internet use severity, appearance anxiety, self-compassion, and image and use of performance-enhancing drugs (IPEDs). Results were compared cross-culturally. The mean time spent online was 5 h (SD = ±3) of daily browsing during the pandemic. The most commonly performed activities included social networking, streaming, and general surfing. A strong association between these online behaviours and appearance anxiety, self-compassion, and IPEDs use was found after adjustment for possible confounders, with higher scores being associated with specific online activities. Significant cross-cultural differences also emerged in terms of the amount of time spent online during the initial stages of the COVID-19 pandemic.
Analysis of Injuries and Wellness in Blind Athletes during an International Football Competition
Authors: Muñoz-Jiménez Jesús , Gámez-Calvo Luisa , Rojas-Valverde Daniel , León Kiko , Gamonales José M.
Published: July 2022

Five-a-side football for blind people is the only adapted football modality present at the Paralympic games. Fa5 is a collaborative-opposition sport in which its participants play with no vision, which causes numerous impacts. At the London 2012 Paralympic Games, it was the sport with the highest incidence of sports injuries. The main objective of this work is to analyse the association between pain perception; spatio-temporal, mechanical, and metabolic workload with injuries; and wellness in players during an international Fa5 competition. The following variables, monitored during an International Fa5 Tournament, were analysed: general well-being, perception of pain and injuries, and the spatio-temporal and metabolic workload. The results show that the incidence of injuries increases as the tournament progresses, where injured players reported greater muscle pain and stress before the matches started. Besides, the players’ internal and external load did not explain the incidence of injury. Still, the values obtained in the wellness questionnaire, the perception of pain, and stress suggested they contributed to the incidence of injury.
Determinants of PrEP Uptake, Intention and Awareness in the Netherlands: A Socio-Spatial Analysis
Authors: Wang Haoyi , Shobowale Oladipupo , den Daas Chantal , Op de Coul Eline , Bakker Bouko , Radyowijati Aryanti , Vermey Koenraad , van Bijnen Arjan , Zuilhof Wim , Jonas Kai J.
Published: July 2022

PrEP uptake in the Netherlands is growing but remains at suboptimal levels. Hence, the analysis of hurdles is paramount. Given the initial focus of PrEP provision among men-who-have-sex-with-men (MSM) via a demonstration project that was launched in June 2015, AmPrEP in Amsterdam, and pharmacies in the main urban areas (so called “Randstad”, entailing Amsterdam, Utrecht, Leiden, The Hague and Rotterdam), investigating regional differences is necessary. This study seeks to unravel regional differences jointly with the psycho-social determinants of PrEP uptake. This cross-sectional study included 3232 HIV-negative MSM recruited via the Dutch subsample of the European-MSM-Internet-Survey in late 2017 (EMIS-2017), which aimed to inform interventions for MSM who are highly affected by infections with HIV and other sexually transmitted infections. Prevalence and the standardised prevalence ratio (SPR) of PrEP awareness, intention and uptake were measured on a regional level (Randstad vs. the rest of the country). Multi-level logistic modelling was conducted to identify the association of PrEP uptake with PrEP awareness and intention, socio-demographic, psycho-social determinants and random effects from regional differences. MSM from the Randstad used more PrEP (SPR = 1.4 vs. 0.7) compared to the rest of the country, but there were minor differences for awareness and intention. The regional distinction was estimated to explain 4.6% of the PrEP use variance. We observed a greater influence from PrEP intention (aOR = 4.5, 95% CI 2.0–10.1), while there was limited influence from the awareness of PrEP (aOR = 0.4, 95% CI 0.04–4.4). Lower education (aOR = 0.4, 95% CI 0.2–0.9) was negatively associated with PrEP uptake; however, no significant difference was found between middle (aOR = 1.2, 95% CI 0.7–2.0) and high education. We showed that regional differences—MSM in non-urban regions—and other psycho-social determinants account for lower PrEP uptake. Based on these findings, more fine-tuned PrEP access with a focus on non-urban regions can be implemented, and tailored campaigns increasing intention/use can be conducted among target populations.
Correction: Messina et al. Knowledge and Practice towards Alcohol Consumption in a Sample of University Students. Int. J. Environ. Res. Public Health 2021, 18, 9528
Authors: Messina Marisa Patrizia , Battagliese Gemma , D’Angelo Alessio , Ciccarelli Rosaria , Pisciotta Fabiola , Tramonte Luigi , Fiore Marco , Ferraguti Giampiero , Vitali Mario , Ceccanti Mauro
Published: July 2022

Gemma Battagliese was not included as an author in the original publication [...]
Night Shift Work Is Associated with Reduced Rate of Humoral Response Following Vaccination for HBV
Authors: Coppeta Luca , Ferrari Cristiana , Trabucco Aurilio Marco , Ferrazza Gianluigi , Magrini Andrea , Rizza Stefano
Published: July 2022

Night shift work has been associated with cardiovascular and metabolic disease, endocrine and immunological disorders. Published studies have reported that a reduced total sleep time with sleep-wake cycle alterations were associated with a reduced rate of humoral response following vaccination. Our study aimed to evaluate the association between night shift work and serological status for HBV among workers employed in a university hospital in Rome. We evaluated medical records of 986 HCWs working at Tor Vergata Policlinic of Rome. We screened all study subjects for anti-HBs IgG, anti-HBc IgG and HBsAg. Serological protection for HBV was evaluated in relation to sex, age group, job task, risk setting and night shift work status. Protective titer was found in 856 (86.8%) study participants and the mean titer was significantly high in females, in subjects aged less than 40 years, in night shift workers and in high-risk setting workers. After adjustment for study covariates, night shift work was no longer associated with an HBV-protective titer. This finding suggests that a vaccination strategy for dampening HBV transmission should be carefully addressed in health care workers (HCWs) doing night shift.
UHPLC/MS-Based Serum Metabolomics Reveals the Mechanism of Radiation-Induced Thrombocytopenia in Mice
Authors: Xiong Ling , Wang Long , Zhang Ting , Ye Xinyuan , Huang Feihong , Huang Qianqian , Huang Xinwu , Wu Jianming , Zeng Jing
Published: July 2022

Radiation-induced thrombocytopenia is a common and life-threatening side effect of ionizing radiation (IR) therapy. However, the underlying pathological mechanisms remain unclear. In the present study, irradiation was demonstrated to significantly reduce platelet levels, inhibit megakaryocyte differentiation, and promote the apoptosis of bone marrow (BM) cells. A metabolomics approach and a UHPLC-QTOF MS system were subsequently employed for the comprehensive analysis of serum metabolic profiles of normal and irradiated mice. A total of 66 metabolites were significantly altered, of which 56 were up-regulated and 10 were down-regulated in irradiated mice compared to normal mice on day 11 after irradiation. Pathway analysis revealed that disorders in glycerophospholipid metabolism, nicotinate and nicotinamide metabolism, sphingolipid metabolism, inositol phosphate metabolism, and tryptophan metabolism were involved in radiation-induced thrombocytopenia. In addition, three important differential metabolites, namely L-tryptophan, LysoPC (17:0), and D-sphinganine, which were up-regulated in irradiated mice, significantly induced the apoptosis of K562 cells. L-tryptophan inhibited megakaryocyte differentiation of K562 cells. Finally, serum metabolomics was performed on day 30 (i.e., when the platelet levels in irradiated mice recovered to normal levels). The contents of L-tryptophan, LysoPC (17:0), and D-sphinganine in normal and irradiated mice did not significantly differ on day 30 after irradiation. In conclusion, radiation can cause metabolic disorders, which are highly correlated with the apoptosis of hematopoietic cells and inhibition of megakaryocyte differentiation, ultimately resulting in thrombocytopenia. Further, the metabolites, L-tryptophan, LysoPC (17:0), and D-sphinganine can serve as biomarkers for radiation-induced thrombocytopenia.
Mechanism-Based Sonodynamic–Chemo Combinations against Triple-Negative Breast Cancer
Authors: Feng Xiaolan , Wu Chen , Yang Wenhao , Wu Jiayi , Wang Pan
Published: July 2022

Due to its noninvasive nature, site-confined irradiation, and high tissue penetrating capabilities, ultrasound (US)-driven sonodynamic treatment (SDT) has been proven to have broad application possibilities in neoplastic and non-neoplastic diseases. However, the inefficient buildup of sonosensitizers in the tumor site remarkably impairs SDT efficiency. The present work proposes a deep-penetrating sonochemistry nanoplatform (Pp18-lipos@SRA737&DOX, PSDL) comprising Pp18 liposomes (Pp18-lipos, Plipo), SRA737 (a CHK1 inhibitor), and doxorubicin (DOX) for the controlled formation of reactive oxygen species (ROS) and release of DOX and SRA737 upon US activation, therefore increasing chemotherapeutic effectiveness and boosting SDT efficacy. Therein, the antitumor activities of DOX have been attributed to its intercalation into the nucleus DNA and induction of cell apoptosis. CHK1 evolved to respond to DNA damage and repair the damage via cell cycle progression. SRA737 is a potent and orally bioavailable clinical drug candidate for inhibiting CHK1, demonstrating adjuvant anticancer effect in vitro and in vivo. It was interesting to find that SRA737 carried into Plipo@DOX could significantly alleviate G2/M cell cycle arrest and aggravate DNA double-strand injuries, resulting in significant cell death. The developed US-switchable nanosystem provides a promising strategy for augmenting sono-chemotherapy against breast cancer controllably and precisely.
Effect of Sintering Temperature on the Properties of CuAlO2 Synthesized from Nanosized Precursors for Application in Smart Infrastructure Systems
Authors: Ghotbi Shabnam , Mousa Mohammed Abbas , Assi Lateef Najeh , Ghahari SeyedAli
Published: July 2022

The current study aimed to evaluate the influence of different sintering temperatures on the properties of copper aluminum oxide (CuAlO2) pellets synthesized from copper oxide (CuO) and aluminum hydroxide (Al(OH)3) for application in smart infrastructure systems. The pellets were sintered at 400 K, 1000 K, and 1300 K, in the presence of nitrogen gas flow to reduce the amount of oxygen availability. The CuAlO2 sintered nanoparticles were chemically analyzed by X-ray diffractometry, and the nanostructure of the materials was studied by scanning electron microscopy. The transmittance of the sintered materials was examined by ultraviolet/visible (UV/Vis) spectrophotometry, and 88% transparency was observed for the pellets sintered at 1300 K. Electrical conductivity was measured at 0.905 mS/cm, indicating a semiconducting behavior.
Diversity of Lacewings (Neuroptera) in an Altitudinal Gradient of the Tacaná Volcano, Southern Mexico
Authors: Cancino-López Rodolfo J. , Moreno Claudia E. , Contreras-Ramos Atilano
Published: July 2022

Neuroptera is an order of insects with a moderate diversity of species numbers yet a high between-family morphological diversity, which has a significant ecological role as a predator. However, there are few studies focused on describing changes in species diversity along environmental gradients. We evaluated changes in the alpha and beta diversity of species and the higher taxa in Neuroptera communities in the Tacaná Volcano in southern Mexico. Five sites each at different altitudes were studied through systematic annual sampling. The taxonomic and phylogenetic alpha diversity were analyzed, as well as the beta diversity and its components, species turnover and nestedness. The alpha diversity had two trends: (1) decreased standardized richness and taxonomic distinctness with increasing altitude, and (2) increased estimated richness and species diversity at intermediate altitudes. The highest turnover values for species, as well as for supra-specific taxa, were recorded at sites with lower altitudes. The highest total beta diversity value was recorded at elevations above 3000 m, whereas the highest number of species and supra-specific taxa were observed at sites between 600 and 2000 m, with an evident decrease above 3000 m. The type of vegetation and environmental conditions may be influencing the decrease in diversity toward higher elevations, which could explain the niche specialization of Neuroptera species to particular sites within the gradient. These results highlight the need to study the environmental factors and their effects on species composition along an elevation gradient.
Optimal Arterial Blood Gas Tensions for the Prognosis of Favorable Neurological Outcomes in Survivors after Extracorporeal Cardiopulmonary Resuscitation
Authors: Hong SungMin , Jang Ji Hoon , Yang Jeong Hoon , Cho Yang Hyun , Ahn Joonghyun , Ryu Jeong-Am
Published: July 2022

Our aim is to assess the optimal levels of oxygen and carbon dioxide for the prognosis of favorable neurologic outcomes in survivors after extracorporeal cardiopulmonary resuscitation (ECPR). We obtained the mean levels of PaCO2 and PaO2 in arterial blood gas samples 72 h after ECPR. The primary outcome was the neurological status, according to the Cerebral Performance Categories (CPC) scale, upon discharge. Of 119 (48.6%) survivors, 95 (38.8%) had favorable neurologic outcomes (CPC 1 or 2). There was a U-shaped relationship between mean arterial blood gas tensions and poor neurological outcomes. The risk of poor neurological outcome was lowest in patients with the second tertile of mean PaCO2 (30–42 mm Hg) and PaO2 (120–160 mm Hg). In a multivariable analysis, third tertile of mean PaCO2, third tertile of mean PaO2, age, shockable rhythm, out of hospital cardiac arrest, duration of cardiopulmonary resuscitation, and ECPR at cardiac catheterization lab were found to be significantly associated with poor neurologic outcomes. Additionally, hypercapnia and extreme hyperoxia were found to be significantly associated with poor neurological outcomes after ECPR. Therefore, maintaining adequate arterial levels of oxygen and carbon dioxide may be important for favorable neurological prognoses in survivors after ECPR.
Fibrinogen to Albumin Ratio as Early Serum Biomarker for Prediction of Intra-Hospital Mortality in Neurosurgical Intensive Care Unit Patients with Spontaneous Intracerebral Hemorrhage
Authors: Bender Michael , Haferkorn Kristin , Tajmiri-Gondai Shahin , Uhl Eberhard , Stein Marco
Published: July 2022

Background: The prognostic value of the fibrinogen to albumin ratio on intrahospital mortality has been investigated in patients with cardiovascular disease, cancer, sepsis, and ischemic stroke; however, it has not been investigated for neurosurgical patients with spontaneous intracerebral hemorrhage (ICH). The present study investigates the impact of the fibrinogen to albumin ratio upon admission for intrahospital mortality in neurosurgical intensive care unit (ICU) patients with spontaneous ICH. Methods: A total of 198 patients with diagnosis of spontaneous ICH treated from 10/2008 to 12/2017 at our ICU were retrospectively analyzed. Blood samples were drawn upon admission, and the patients’ demographic, medical data, and cranial imaging were collected. Binary logistic regression analysis was performed to identify independent prognostic factors for intrahospital mortality. Results: The total rate of intrahospital mortality was 35.4% (n = 70). In the multivariate regression analysis, higher fibrinogen to albumin ratio (OR = 1.16, CI = 1.02–1.31, p = 0.03) upon admission was an independent predictor of intrahospital mortality in neurosurgical ICU patients with ICH. Moreover, a fibrinogen to albumin ratio cut-off level of >0.075 was related to increased intrahospital mortality (Youden’s index = 0.26, sensitivity = 0.51, specificity = 0.77). Conclusion: A fibrinogen to albumin ratio > 0.075 was significantly associated with increased intrahospital mortality in ICH patients.
Effect of Sirolimus vs. Everolimus on CMV-Infections after Kidney Transplantation—A Network Meta-Analysis
Authors: Wolf Sebastian , Hoffmann Verena S. , Sommer Florian , Schrempf Matthias , Li Mingming , Ryll Martin , Wirth Ulrich , Ilmer Matthias , Werner Jens , Andrassy Joachim
Published: July 2022

(1) Background: Following renal transplantation, infection with cytomegalovirus (CMV) is a common and feared complication. mTOR-inhibitor (mTOR-I) treatment, either alone or in combination with calcineurininhibitors (CNIs), significantly reduces the CMV incidence after organ transplantation. As of now, there is no information on which mTOR-I, sirolimus (SIR) or everolimus (ERL), has a stronger anti-CMV effect. (2) Methods: The current literature was searched for prospective randomized controlled trials in renal transplantation. There were 1164 trials screened, of which 27 could be included (11,655 pts.). We performed a network meta-analysis to analyze the relative risk of different types of mTOR-I treatment on CMV infection 12 months after transplantation compared to CNI treatment. (3) Results: Four different types of mTOR-I treatment were analyzed in network meta-analyses—SIR mono, ERL mono, SIR with CNI, ERL with CNI. The mTOR-I treatment with the strongest anti-CMV effect compared to a regular CNI treatment was ERL in combination with a CNI (relative risk (RR) 0.27, confidence interval (CI) 0.22–0.32, p < 0.0001). The other mTOR-I therapy groups showed a slightly decreased anti-CMV efficacy (SIR monotherapy (mono): RR 0.35, CI 0.22–0.57, p < 0.001; SIR with CNI: RR 0.43, CI 0.29–0.64, p < 0.0001; ERL mono: RR 0.46, CI 0.22–0.93, p = 0.031). (4) Conclusions: The anti-CMV effect of both mTOR-Is (SRL and ERL) is highly effective, irrespective of the combination with other immunosuppressive drugs. Certain differences with respect to the potency against the CMV could be found between SRL and ERL. Data gained from this analysis seem to support that a combination of ERL and CNI has the most potent anti-CMV efficacy.
Hydrogen Refueling Stations and Carbon Emission Reduction of Coastal Expressways: A Deployment Model and Multi-Scenario Analysis
Authors: Wang Zhe , Wang Dongxing , Zhao Fan , Han Fenghui , Ji Yulong , Cai Wenjian
Published: July 2022

Hydrogen is considered to the ultimate solution to achieve carbon emission reduction due to its wide sources and high calorific value, as well as non-polluting, renewable, and storable advantages. This paper starts from the coastal areas, uses offshore wind power hydrogen production as the hydrogen source, and focuses on the combination of hydrogen supply chain network design and hydrogen expressway hydrogen refueling station layout optimization. It proposes a comprehensive mathematical model of hydrogen supply chain network based on cost analysis, which determined the optimal size and location of hydrogen refueling stations on hydrogen expressways in coastal areas. Under the multi-scenario and multi-case optimization results, the location of the hydrogen refueling station can effectively cover the road sections of each case, and the unit hydrogen cost of the hydrogen supply chain network is between 11.8 and 15.0 USD/kgH2. Meanwhile, it was found that the transportation distance and the number of hydrogen sources play a decisive role on the cost of hydrogen in the supply chain network, and the location of hydrogen sources have a decisive influence on the location of hydrogen refueling stations. In addition, carbon emission reduction results of hydrogen supply chain network show that the carbon emission reduction per unit hydrogen production is 15.51 kgCO2/kgH2 at the production side. The CO2 emission can be reduced by 68.3 kgCO2/km and 6.35 kgCO2/kgH2 per unit mileage and per unit hydrogen demand at the application side, respectively. The layout planning utilization of hydrogen energy expressway has a positive impact on energy saving and emission reduction.
DdaCrz1, a C2H2-Type Transcription Factor, Regulates Growth, Conidiation, and Stress Resistance in the Nematode-Trapping Fungus Drechslerella dactyloides
Authors: Zhao Xiaozhou , Fan Yani , Xiang Meichun , Kang Seogchan , Wang Shunxian , Liu Xingzhong
Published: July 2022

The Ca2+/calmodulin-dependent signaling pathway regulates diverse cellular processes. Calcineurin is a calcium-dependent phosphatase acting in fungi mainly through Crz1, a zinc finger transcription factor. Although the likely involvement of Ca2+ in fungal carnivorism has been documented, how Crz1 functions in nematode-trapping fungi remains unknown. Here, we identified the Crz1 gene (named as DdaCrz1) in Drechslerella dactyloides, a species that forms constricting rings to trap nematodes. The deletion of DdaCrz1 significantly reduced hyphal growth and conidiation, trap formation, and ring cell inflation. Moreover, the mutation increased sensitivity to Mn2+ but decreased sensitivity to Ca2+, Mg2+, Zn2+, and Li+. Similarly, the mutant showed increased tolerance to osmotic stress but was more sensitive to Congo red, a cell wall-damaging agent. Our results confirmed the critical roles of the Ca2+/calmodulin-dependent signaling pathway in regulating growth, conidiation, and the stress response, and suggested its involvement in trapping nematodes.
Macrophage Lysosomal Alkalinization Drives Invasive Aspergillosis in a Mouse Cystic Fibrosis Model of Airway Transplantation
Authors: Matthaiou Efthymia Iliana , Chiu Wayland , Conrad Carol , Hsu Joe
Published: July 2022

Cystic fibrosis (CF) lung transplant recipients (LTRs) exhibit a disproportionately high rate of life-threatening invasive aspergillosis (IA). Loss of the cystic fibrosis transmembrane conductance regulator (CFTR-/-) in macrophages (mφs) has been associated with lyosomal alkalinization. We hypothesize that this alkalinization would persist in the iron-laden post-transplant microenvironment increasing the risk of IA. To investigate our hypothesis, we developed a murine CF orthotopic tracheal transplant (OTT) model. Iron levels were detected by immunofluorescence staining and colorimetric assays. Aspergillus fumigatus (Af) invasion was evaluated by Grocott methenamine silver staining. Phagocytosis and killing of Af conidia were examined by flow cytometry and confocal microscopy. pH and lysosomal acidification were measured by LysoSensorTM and LysotrackerTM, respectively. Af was more invasive in the CF airway transplant recipient compared to the WT recipient (p < 0.05). CFTR-/- mφs were alkaline at baseline, a characteristic that was increased with iron-overload. These CFTR-/- mφs were unable to phagocytose and kill Af conidia (p < 0.001). Poly(lactic-co-glycolic acid) (PLGA) nanoparticles acidified lysosomes, restoring the CFTR-/- mφs’ ability to clear conidia. Our results suggest that CFTR-/- mφs’ alkalinization interacts with the iron-loaded transplant microenvironment, decreasing the CF-mφs’ ability to kill Af conidia, which may explain the increased risk of IA. Therapeutic pH modulation after transplantation could decrease the risk of IA.
The Effect of Statin on Anemia in Patients with Chronic Kidney Disease and End-Stage Kidney Disease: A Systematic Review and Meta-Analysis
Authors: Tsai Meng-Hsu , Su Fu-You , Chang Hao-Yun , Su Po-Cheng , Chiu Li-Yun , Nowicki Michal , Kao Chih-Chin , Lin Yen-Chung
Published: July 2022

Although erythropoietin-stimulating agents are effective in treating anemia in patients with end-stage kidney disease (ESKD) undergoing hemodialysis, some ESKD patients, especially those with inflammation, continue to suffer from anemia. Statin, an inhibitor of hydroxymethylglutaryl-CoA (HMG-CoA) reductase with lipid-lowering effects, may have a pleiotropic effect in reducing inflammation, and thus increase hemoglobin (Hb) level. We searched the PubMed, Embase, and Cochrane databases for relevant studies. The population of interest comprised advanced chronic kidney disease (CKD) patients and ESKD patients receiving hemodialysis with statin treatment. The included study designs were randomized control trial/cohort study/pre-post observational study, and outcomes of interest were Hb, erythropoietin resistance index (ERI) and ferritin. PRISMA 2020 guidelines were followed, and risk of bias (RoB) was assessed using the RoB 2.0 tool in randomized controlled trials, and the Newcastle-Ottawa scale (NOS) in cohort studies. We eventually included ten studies (5258 participants), comprising three randomized controlled trials and seven cohort studies. Overall, Hb increased by 0.84 g/dL (95% confidence interval [CI]: −0.02 to 1.70) in all groups using statins, including single-arm cohorts, and by 0.72 g/dL (95% CI: −0.02 to 1.46) in studies with placebo control. Hb levels were higher in the study group than in the control group, with a mean difference of 0.18 g/dL (95% CI: 0.04–0.32) at baseline and 1.0 g/dL (95% CI: 0.13–1.87) at the endpoint. Ferritin increased by 9.97 ng/mL (95% CI: −5.36 to 25.29) in the study group and decreased by 34.01 ng/mL (95% CI: −148.16 to 80.14) in the control group; ferritin fluctuation was higher in the control group. In conclusion, statin may improve renal anemia in ESKD patients receiving hemodialysis and regular erythropoietin-stimulating agents. Future studies with more rigorous methodology and larger sample size study should be performed to confirm this beneficial effect.
Comprehensive Genomic Profiling in the Management of Ovarian Cancer—National Results from Croatia
Authors: Čerina Dora , Matković Višnja , Katić Kristina , Belac Lovasić Ingrid Belac , Šeparović Robert , Canjko Ivana , Bajić Žarko , Vrdoljak Eduard
Published: July 2022

Today, in the era of precision medicine, the determination of genomic instability or other potentially targetable mutations, along with BRCA 1 and BRCA 2, is a crucial component of the diagnosis and treatment management of advanced ovarian cancer. Advanced technologies such as next-generation sequencing (NGS) have enabled comprehensive genomic profiling (CGP) analysis to become more feasible for routine use in daily clinical work. Here, we present the results for the first two years of an analysis of patients with advanced ovarian cancer on a national level. The aim was to establish the position of CGP in the daily clinical practice of treating ovarian cancer. We performed a multicenter, retrospective, cross-sectional analysis on the total population of Croatian patients who were newly diagnosed with locally advanced or metastatic ovarian cancer or whose initial disease had progressed from 1 January 2020 to 1 December 2021, and whose tumors underwent CGP analysis. All 86 patients (100%) analyzed with CGP had at least one genomic alteration (GA). The median LOH was 14.6 (IQR 6.8–21.7), with 35 patients (41%) having an LOH ≥ 16. We found BRCA-positive status in 22 patients (26%). Conventional testing, which detects only BRCA mutations, would have opted for therapy with PARP inhibitors in 22 (26%) of our patients. However, CGP revealed the need for PARP inhibitors in 35 patients (41%). The results identified a significantly higher number of women who would achieve a possible benefit from targeted therapy. Hence, we believe that CGP should be a backbone diagnostic tool in the management of ovarian cancer.
Species Composition Affects the Accuracy of Stand-Level Biomass Models in Hemiboreal Forests
Authors: Liepiņš Jānis , Lazdiņš Andis , Kalēja Santa , Liepiņš Kaspars
Published: July 2022

Various tree species contribute differently to total biomass stock, making the development of species-specific stand-level equations critical for better estimation of forest biomass and quantification of carbon stocks. Previously derived dry weight biomass models did not assess the effect of dominant species composition according to stand growing stock. Growing stock definitions and forest species composition differ by country, justifying the need for national stand-level biomass equations. We explored the relationship between growing stock volume and stand biomass density of above- and below-ground components in six common forest categories in Latvia using plot-level data from the National Forest Inventory from 2016 to 2020. Additionally, we explored model dependence on region, forest type, and species composition index. Models that considered growing stock and dominant species composition index performed better than models with growing stock as the only variable, especially for heterogeneous deciduous forests with greater species diversity. The elaborated models are a useful alternative to individual-level assessment for estimating forest biomass stocks in circumstances where individual tree data are not available.
Elastic Properties and Hardness of Mixed Alkaline Earth Silicate Oxynitride Glasses
Authors: Ali Sharafat
Published: July 2022

The incorporation of nitrogen as a second anion species into oxide glasses offers unique opportunities for modifying glass properties via changes in glass polymerization and structure. In this work, the compositional dependence of elastic properties and the nanoindentation hardness of mixed alkaline-earth silicate oxynitride glasses containing a high amount of nitrogen (>15 at.%, c.a. 35 e/o) were investigated. Three series of silicon oxynitride glass compositions AE–Ca–Si–O–N glasses (where AE = Mg, Sr, and Ba) having varying amounts of modifiers were prepared using a new glass synthesis route, in which a precursor powder of metal hydrides was used. The obtained glasses contained high amounts of N (19 at.%, c.a. 43 e/o) and modifier cations (26 at.%, c.a. 39 e/o). Mg–Ca–Si–O–N glasses had high values of nanohardness (12–16 GPa), along with a reduced elastic modulus (130–153 GPa) and Young’s modulus (127–146 GPa), in comparison with the Sr–Ca- and Ba–Ca-bearing oxynitride glasses. Both the elastic modulus and the nanohardness of AE–Ca–Si–O–N glasses decreased with an increase in the atomic number of the AE element. These property changes followed a linear dependence on the effective cation field strength (ECFS) of the alkaline earth (AE) modifier, according to their valences and ionic radii. No mixed alkaline-earth effect was observed in the current investigation, indicating that the properties were more dictated by the nitrogen content.
The Internet Shopping Optimization Problem with Multiple Item Units (ISHOP-U): Formulation, Instances, NP-Completeness, and Evolutionary Optimization
Authors: Ornelas Fernando , Santiago Alejandro , Martínez Salvador Ibarra , Ponce-Flores Mirna Patricia , Terán-Villanueva Jesús David , Balderas Fausto , Rocha José Antonio Castán , García Alejandro H. , Laria-Menchaca Julio , Treviño-Berrones Mayra Guadalupe
Published: July 2022

In this work, we investigate the variant of the Internet Shopping Optimization Problem (ISHOP) that considers different item units. This variant is more challenging than the original problem. The original ISHOP is already known as a combinatorial NP-hard problem. In this work, we present a formal proof that the ISHOP variant considering different item units belongs to the NP-Hard complexity class. The abovementioned variant is familiar to companies and consumers who need to purchase more than one unit of a specific product to satisfy their requirements. For example, companies buy different quantities of construction materials, medical equipment, office supplies, or chemical components. We propose two new evolutionary operators (crossover and mutation) and an unfeasible solution repair method for the studied ISHOP variant. Furthermore, we produce a new benchmark of 15 synthetic instances where item prices follow a random uniform distribution. Finally, to assess our evolutionary operators, we implemented two Evolutionary Algorithms, a Genetic Algorithm (GA) and a Cellular Genetic Algorithm (CGA), and an experimental evaluation against a Water Cycle Algorithm (WCA) from the state-of-the-art. Experimental results show that our proposed GA performs well with statistical significance.
On Geometric Properties of Bessel–Struve Kernel Functions in Unit Disc
Authors: Alarifi Najla M. , Mondal Saiful R.
Published: July 2022

The Bessel–Struve kernel function defined in the unit disc is used in this study. The Bessel–Struve kernel functions are generalized in this article, and differential equations are derived. We found conditions under which the generalized Bessel–Struve function is Lemniscate convex by using a subordination technique. The relation between the Janowski class and exponential class is also derived.
Periostin Contributes to Fibrocartilage Layer Growth of the Patella Tendon Tibial Insertion in Mice
Authors: Mutsuzaki Hirotaka , Yoshida Yuta , Nakajima Hiromi
Published: July 2022

Background and Objectives: The influence of periostin on the growth of the patella tendon (PT) tibial insertion is unknown. The research described here aimed to reveal the contribution of periostin to the growth of fibrocartilage layers of the PT tibial insertion using periostin knockout mice. Materials and Methods: In both the wild-type (WD; C57BL/6N, periostin +/+; n = 54) and periostin knockout (KO; periostin −/−; n = 54) groups, six mice were euthanized on day 1 and at 1, 2, 3, 4, 6, 8, 10, and 12 weeks of age. Chondrocyte proliferation and apoptosis, number of chondrocytes, safranin O-stained glycosaminoglycan (GAG) area, staining area of type II collagen, and length of the tidemark were investigated. Results: Chondrocyte proliferation and apoptosis in KO were lower than those in WD on day 1 and at 1, 4, and 8 weeks and on day 1 and at 4, 6, and 12 weeks, respectively. Although the number of chondrocytes in both groups gradually decreased, it was lower in KO than in WD on day 1 and at 8 and 12 weeks. In the extracellular matrix, the GAG-stained area in KO was smaller than that in WD on day 1 and at 1, 4, 8, 10, and 12 weeks. The staining area of type II collagen in KO was smaller than that in WD at 8 weeks. The length of the tidemark in KO was shorter than that in WD at 4 and 6 weeks. Conclusion: Loss of periostin led to decreased chondrocyte proliferation, chondrocyte apoptosis, and the number of chondrocytes in the growth process of the PT tibial insertion. Moreover, periostin decreased and delayed GAG and type II collagen production and delayed tidemark formation in the growth process of the PT tibial insertion. Periostin can, therefore, contribute to the growth of fibrocartilage layers in the PT tibial insertion. Periostin deficiency may result in incomplete growth of the PT tibial insertion.
β-Glucan-Functionalized Nanoparticles Down-Modulate the Proinflammatory Response of Mononuclear Phagocytes Challenged with Candida albicans
Authors: Lima Tânia , Gunnarsson Stefán B. , Coelho Elisabete , Evtuguin Dmitry V. , Correia Alexandra , Coimbra Manuel A. , Cedervall Tommy , Vilanova Manuel
Published: July 2022

Systemic fungal infections are associated with significant morbidity and mortality, and Candida albicans is the most common causative agent. Recognition of yeast cells by immune cell surface receptors can trigger phagocytosis of fungal pathogens and a pro-inflammatory response that may contribute to fungal elimination. Nevertheless, the elicited inflammatory response may be deleterious to the host by causing excessive tissue damage. We developed a nanoparticle-based approach to modulate the host deleterious inflammatory consequences of fungal infection by using β1,3-glucan-functionalized polystyrene (β-Glc-PS) nanoparticles. β-Glc-PS nanoparticles decreased the levels of the proinflammatory cytokines TNF-α, IL-6, IL-1β and IL-12p40 detected in in vitro culture supernatants of bone marrow-derived dendritic cells and macrophage challenged with C. albicans cells. Moreover, β-Glc-PS nanoparticles impaired the production of reactive oxygen species by bone marrow-derived dendritic cells incubated with C. albicans. This immunomodulatory effect was dependent on the nanoparticle size. Overall, β-Glc-PS nanoparticles reduced the proinflammatory response elicited by fungal cells in mononuclear phagocytes, setting the basis for a targeted therapy aimed at protecting the host by lowering the inflammatory cost of infection.
Sex Differences in the Relationship between Excessive Alcohol Consumption and Metabolic Abnormalities: A Community-Based Study in Taiwan
Authors: Wang Liang-Jen , Lin Chih-Lang , Chen Yi-Chih , Lin Chemin , Shyu Yu-Chiau , Chen Chih-Ken
Published: July 2022

Excessive alcohol consumption, as part of an unhealthy lifestyle, can contribute to metabolic abnormalities. This study investigated the sex differences in the relationship between excessive drinking and the risk of metabolic abnormalities. This community-based study included 3387 participants (age range: 30–103 years, mean age ± SD: 57 ± 13.5 years, 38.2% males) from the northeastern region of Taiwan. All participants completed a demographic survey and were subjected to blood tests. The risks of excessive drinking were evaluated using the Alcohol Use Disorder Identification Test (AUDIT). The results showed that males were at higher risks of obesity, hypertension, and hypertriglyceridemia, but at a lower risk of abdominal obesity than females. Males with hazardous drinking were at greater risks of hypertension, hyperglycemia, low serum levels of high-density lipoprotein cholesterol, and hypertriglyceridemia compared to those with no drinking. Females with hazardous drinking were at a greater risk of hypertension than those with no drinking. There was no interaction effect of sex and excessive drinking on the risks of metabolic abnormalities after controlling for demographics and lifestyle-related habits. Future studies are warranted to explore the sex-specific risk factors for metabolic abnormalities and to elucidate the mechanism underlying this association between alcohol consumption and metabolic abnormalities.
Ischemic Stroke and Dietary Vitamin B12 Deficiency in Old-Aged Females: Impaired Motor Function, Increased Ischemic Damage Size, and Changed Metabolite Profiles in Brain and Cecum Tissue
Authors: Poole Joshua , Jasbi Paniz , Pascual Agnes S. , North Sean , Kwatra Neha , Weissig Volkmar , Gu Haiwei , Bottiglieri Teodoro , Jadavji Nafisa M.
Published: July 2022

A vitamin B12 deficiency (vit. B12 def.) is common in the elderly, because of changes in metabolism. Clinical studies have reported that a vit. B12 def. results in worse outcome after stroke, and the mechanisms through which a vit. B12 def. changes the brain requires further investigation. This study investigated the role of vit. B12 def. on stroke outcome and mechanisms using aged female mice. Eighteen-month-old females were put on a control or vit. B12 def. diet for 4 weeks, after which an ischemic stroke was induced in the sensorimotor cortex. After damage, motor function was measured, the animals were euthanized, and tissues were collected for analysis. Vit. B12 def. animals had increased levels of total homocysteine in plasma and liver, and choline levels were also increased in the liver. Vit. B12 def. animals had larger damage volume in brain tissue and more apoptosis. The cecum tissue pathway analysis showed dysfunction in B12 transport. The analysis of mitochondrial metabolomics in brain tissue showed reduced levels of metabolites involved in the TCA cycle in vit. B12 def. animals. Motor function after stroke was impaired in vit. B12 def. animals. A dietary vit. B12 def. impairs motor function through increased apoptosis and changes in mitochondrial metabolism in brain tissue.
Druggable Targets and Compounds with Both Antinociceptive and Antipruritic Effects
Authors: Weng Hao-Jui , Pham Quoc Thao Trang , Chang Chia-Wei , Tsai Tsen-Fang
Published: July 2022

Pain and itch are both important manifestations of various disorders, such as herpes zoster, atopic dermatitis, and psoriasis. Growing evidence suggests that both sensations have shared mediators, overlapping neural circuitry, and similarities in sensitization processes. In fact, pain and itch coexist in some disorders. Determining pharmaceutical agents and targets for treating pain and itch concurrently is of scientific and clinical relevance. Here we review the neurobiology of pain and itch and discuss the pharmaceutical targets as well as novel compounds effective for the concurrent treatment of these sensations.
Research Progress and Potential Applications of Spermidine in Ocular Diseases
Authors: Han Wentao , Li Haoyu , Chen Baihua
Published: July 2022

Spermidine, a natural polyamine, exists in almost all human tissues, exhibiting broad properties like anti-aging, autophagy induction, anti-inflammation, anti-oxidation, cell proliferation activation, and ion channel regulation. Considering that spermidine is already present in human nutrition, recent studies targeting supplementing exogenous sources of this polyamine appear feasible. The protective role of spermidine in various systems has been illuminated in the literature, while recent progress of spermidine administration in ocular diseases remains to be clarified. This study shows the current landscape of studies on spermidine and its potential to become a promising therapeutic agent to treat ocular diseases: glaucoma, optic nerve injury, age-related macular degeneration (AMD), cataracts, dry eye syndrome, and bacterial keratitis. It also has the potential to become a potent biomarker to predict keratoconus (KC), cataracts, uveitis, glaucoma, proliferative diabetic retinopathy (PDR), proliferative vitreoretinopathy (PVR), and retinopathy of prematurity (ROP). We also summarize the routes of administration and the effects of spermidine at different doses.
A Simulator and First Reinforcement Learning Results for Underwater Mapping
Authors: Rosynski Matthias , Buşoniu Lucian
Published: July 2022

Underwater mapping with mobile robots has a wide range of applications, and good models are lacking for key parts of the problem, such as sensor behavior. The specific focus here is the huge environmental problem of underwater litter, in the context of the Horizon 2020 SeaClear project, where a team of robots is being developed to map and collect such litter. No reinforcement-learning solution to underwater mapping has been proposed thus far, even though the framework is well suited for robot control in unknown settings. As a key contribution, this paper therefore makes a first attempt to apply deep reinforcement learning (DRL) to this problem by exploiting two state-of-the-art algorithms and making a number of mapping-specific improvements. Since DRL often requires millions of samples to work, a fast simulator is required, and another key contribution is to develop such a simulator from scratch for mapping seafloor objects with an underwater vehicle possessing a sonar-like sensor. Extensive numerical experiments on a range of algorithm variants show that the best DRL method collects litter significantly faster than a baseline lawn mower trajectory.