bluuebunny/crossref_metadata_2025 huggingface dataset:
Wheat Nutrient Management Strategies to Increase Productivity, Profitability and Quality on Sandy Loam Soils
Authors: Jat Lali , Naresh Rama Krishna , Bhatt Rajan , Chandra Mandapelli Sharath , Singh Sanskriti , Gupta S. K. , Alataway Abed , Dewidar Ahmed Z. , Mattar Mohamed A.
Published: November 2022

By 2050, the population of the world is anticipated to increase from 7.7 billion to 9.7 billion people, and, and wheat is expected to continue to play a vital role in ensuring food security globally. It is the main diet for 40% of the world’s population and supplies food for more than 4.5 billion people in 94 countries contains 21% of the joules and 20% of the protein. The present investigations were carried out during rabi 2017–2018 and 2018–2019 to determine how optimal nutrient management (INM) practices enhance fertilizer usage efficiency, productivity, soil health, and viability in wheat (Variety DBW 71) through innovative nutritional sources and their modes of application methods. The treatments comprised of, viz., control, basal applications of recommended NPK (80:60:40)/NPK granules (200 kg/ha) + FYM (5 t ha−1) + bio-stimulant granules (62.5 kg/ha), +NPK bio-fertilizer (seed treatment), along with a top dressing of urea (20 kg/ha)/bio-stimulant (625 mL ha−1)/NPK Powder (1%) sprays (40/55/70 DAS), which had triplicated randomized block design (RBD) at the crop research farm of SVPUA&T, Meerut (U.P.). The results revealed that wheat grown with incorporation of FYM and bio-stimulant –L attained significantly better growth and higher dry matter accumulation across the stages. The crop contained 1.63% N, 0.31% P, 0.69% K in grain, and 0.57% N, 0.11% P and 1.34% K in straw. Such crops exhibited agronomic, physiological, and apparent recovery efficiency of NPK of the order of 3.2 kg kg−1 of nutrient applied, 14.0 kg kg−1 of nutrient uptake, and 0.23% against recommended NPK. Applications of FYM, NPK bio-fertilizer+ urea, and bio-stimulant + NPK sprays worked synergistically and increased grain yields by 29.8, 35.2, 50.3 and 41.1% over their respective controls. The results also indicated that soil organic carbon (0.47%), available NPK (227.0, 27.7, 172.1 kg/ha), dehydrogenase activity, and microbial population (bacteria, fungal and actinomycetes) in soil was also highest with the treatment. Finally, the wheat crop required an investment of Rs ha−1 131,453 and fetched a net reoccurrence of Rs. 96,154, with benefits of Rs. 3.72 over cost, respectively. Therefore, the study reveals that integrated nutrient management, viz., FYM 5 t ha−1 +NPK (12–32–16) -G @ 200 kg/ha + NPK bio-fertilizer (seed treatment) + urea @ 20 kg/ha, foliar application NPK (18−18−18) -P@ 1% and bio-stimulant –L 0.62 L ha−1 improved the better growth, productivity, soil health and profitability of wheat crops. Finally, to boost production, the region must emphasize the wheat crop’s part in integrated nutrition management with foliar application of bio-stimulants. Furthermore, these investigations must be reinvestigated at different sites with different agro-climatic conditions and texturally divergent soils.
Changes in the Physical, Chemical, and Bacterial Community Characteristics of Soil in Response to Short-Term Combined Organic–Inorganic Fertilizers in a Dry Direct-Seeded Paddy Field
Authors: Guo Xiaohong , Zou Bing , Xu Lingqi , Zhang Jianing , Zheng Guiping , Wang Haize , Yin Dawei , Li Hongyu , Zhang Wenzhong , Lv Yandong , Zhao Minghui
Published: November 2022

Dry direct-seeded rice cultivation is a simple and labor-saving planting method wherein the combined application of organic and inorganic fertilizers can improve yield. However, the effects of combined fertilizers on soil properties and bacteria in dry direct-seeded rice paddy soil are unclear. Here, four treatments, conventional fertilization (NPK), seaweed bio-organic fertilizer + NPK, Jishiwang bio-organic fertilizer + NPK, and attapulgite organic fertilizer + NPK applied for three consecutive years were tested to explore their effects on soil physical, chemical, and bacterial community characteristics in a dry direct-seeded rice paddy field. The combined fertilizers increased alkaline hydrolysis-nitrogen and available potassium while decreasing the bulk density and pH; in addition, a marked increase in the relative abundance of soil macroaggregates (>5 mm) and clay particles and a decrease in that of sand was observed. Urease and neutral phosphatase activities were the highest with the Jishiwang organic fertilizer + NPK, whereas invertase and catalase activities were the highest with attapulgite organic fertilizer + NPK. All combined fertilizers considerably increased the bacterial richness index (ACE) and Chao index; Jishiwang bio-organic fertilizer + NPK also increased the Simpson index, whereas the seaweed bio-organic fertilizer + NPK reduced it. Proteobacteria and Acidobacteria accounted for 54.25–70.49% of the total bacterial relative abundance. The relative abundance of Verrucomicrobia, Chloroflexi, Firmicutes, and Nitrospirae increased with the combined fertilizers. The correlation network analysis showed predominant antagonistic relationships. A redundancy analysis demonstrated that total nitrogen, soil organic matter, urease, and invertase were the main environmental factors affecting bacterial composition. Combined fertilizers may improve soil physical and chemical properties, fertility, and bacterial richness.
BUBR1 as a Prognostic Biomarker in Canine Oral Squamous Cell Carcinoma
Authors: Delgado Leonor , Monteiro Luís , Silva Patrícia , Bousbaa Hassan , Garcez Fernanda , Silva João , Brilhante-Simões Paula , Pires Isabel , Prada Justina
Published: November 2022

Chromosomal instability (CIN) plays a key role in the carcinogenesis of several human cancers and can be related to the deregulation of core components of the spindle assembly checkpoint (SAC) including BUBR1 protein kinase. These proteins have been related to tumor development and poor survival rates in human patients with oral squamous cell carcinoma (OSCC). To investigate the expression of the SAC proteins BUBR1, BUB3 and SPINDLY and also Ki-67 in canine OSCC, we performed an immunohistochemical evaluation in 60 canine OSCCs and compared them with clinical and pathological variables. BUBR1, Ki-67, BUB3 and SPINDLY protein expressions were detected in all cases and classified as with a high-expression extent score in 31 (51.7%) cases for BUBR1, 33 (58.9%) cases for BUB3 and 28 (50.9%) cases for SPINDLY. Ki-67 high expression was observed in 14 (25%) cases. An independent prognostic value for BUBR1 was found, where high BUBR1 expression was associated with lower survival (p = 0.012). These results indicate that BUBR1 expression is an independent prognostic factor in these tumors, suggesting the potential use for clinical applications as a prognostic biomarker and also as a pharmacological target in canine OSCC.
Oral Administration of a Phage Cocktail to Reduce Salmonella Colonization in Broiler Gastrointestinal Tract—A Pilot Study
Authors: Pelyuntha Wattana , Yafa Ananya , Ngasaman Ruttayaporn , Yingkajorn Mingkwan , Chukiatsiri Kridda , Champoochana Nidanut , Vongkamjan Kitiya
Published: November 2022

Salmonella contamination in poultry meat products can lead to serious foodborne illness and economic loss from product recalls. It is crucial to control Salmonella contamination in poultry from farm to fork. Bacteriophages (phages) are viruses of bacteria that offer several advantages, especially their specificity to target bacteria. In our study, three Salmonella phages (vB_SenS_KP001, vB_SenS_KP005, and vB_SenS_WP110) recovered from a broiler farm and wastewater treatment stations showed high lysis ability ranging from 85.7 to 96.4% on over 56 serovars of Salmonella derived from several sources, including livestock and a broiler farm environment. A three-phage cocktail reduced S. Enteritidis and S. Typhimurium, in vitro by 3.9 ± 0.0 and 3.9 ± 0.2 log units at a multiplicity of infection (MOI) of 103 and 3.8 ± 0.4 and 4.1 ± 0.2 log units at MOI of 104 after 6 h post-phage treatment. A developed phage cocktail did not cause phage resistance in Salmonella during phage treatments for three passages. Phages could survive under simulated chicken gastrointestinal conditions in the presence of gastric acid for 2 h (100.0 ± 0.0% survivability), bile salt for 1 h (98.1 ± 1.0% survivability), and intestinal fluid for 4 h (100 ± 0.0% survivability). Each phage was in the phage cocktail at a concentration of up to 9.0 log PFU/mL. These did not cause any cytotoxicity to human fibroblast cells or Caco-2 cells as indicated by the percent of cell viability, which remained nearly 100% as compared with the control during 72 h of co-culture. The phage cocktail was given to broilers raised in commercial conditions at a 9 log PFU/dose for five doses, while naturally occurring Salmonella cells colonized in the gastrointestinal tract of broilers were significantly reduced as suggested by a considerably lower Salmonella prevalence from over 70 to 0% prevalence after four days of phage treatment. Our findings suggest that a phage cocktail is an effective biocontrol agent to reduce Salmonella present in the guts of broilers, which can be applied to improve food safety in broiler production.
The Mechanisms of Fur Development and Color Formation in American Mink Revealed Using Comparative Transcriptomics
Authors: Wang Lidong , Zhou Shengyang , Liu Guangshuai , Lyu Tianshu , Shi Lupeng , Dong Yuehuan , He Shangbin , Zhang Honghai
Published: November 2022

American mink fur is an important economic product, but the molecular mechanisms underlying its color formation and fur development remain unclear. We used RNA-seq to analyze the skin transcriptomes of young and adult mink with two different hair colors. The mink comprised black adults (AB), white adults (AW), black juveniles (TB), and white juveniles (TW) (three each). Through pair comparison and cross-screening among different subgroups, we found that 13 KRTAP genes and five signaling pathways (the JAK–STAT signaling pathway (cfa04630), signaling pathways regulating pluripotency of stem cells (cfa04550), ECM–receptor interaction (cfa04512), focal adhesion (cfa04510), and the Ras signaling pathway (cfa04014)) were related to mink fur development. We also found that members of a tyrosinase family (TYR, TYRP1, and TYRP2) are involved in mink hair color formation. The expression levels of TYR were higher in young black mink than in young white mink, but this phenomenon was not observed in adult mink. Our study found significant differences in adult and juvenile mink skin transcriptomes, which may shed light on the mechanisms of mink fur development. At the same time, the skin transcriptomes of black and white mink also showed differences, with the results varying by age, suggesting that the genes regulating hair color are active in early development rather than in adulthood. The results of this study provide molecular support in breeding for mink coat color and improving fur quality.
From Keratoma to Anaplastic Malignant Melanoma in a Horse’s Hoof
Authors: Stefanik Elżbieta , Górski Kamil , Turek Bernard , Drewnowska-Szczepakowska Olga , Kliczkowska-Klarowicz Katarzyna , Stefanik Aleksandra
Published: November 2022

Melanomas in horses are most often associated with gray, older horses with an average age of over 16 years. Anaplastic malignant melanoma, however, can very rarely affect non-gray horses. Herein, we report a case of a 16-year-old Wielkopolski gelding with a chronic lameness caused by a mass in the hoof. The first resection of the lesion and histopathological examination confirmed the presence of a keratoma. The regrown mass and persistent lameness resulted in another mass resection. The second histopathological examination result suggested a neoplastic growth of melanocytic origin with a low histological malignancy. Less than 2 years after the first resection, the horse returned to the clinic with deformation of the hoof capsule and severe lameness. The result of the third histopathological examination indicated low-differentiated malignant neoplasm. The result of the immunohistochemically test indicates a tumor of melanocytic origin with high malignancy.
Ventilator-Assisted Inspiratory and Expiratory Breath-Hold Thoracic Computed Tomographic Scans Can Detect Dynamic and Static Airway Collapse in Dogs with Limited Agreement with Tracheobronchoscopy
Authors: Levy Alice , Reinero Carol , Masseau Isabelle
Published: November 2022

Airway collapse (AC) in dogs includes a tracheal collapse, mainstem and lobar bronchial collapse, and bronchomalacia (i.e., segmental/subsegmental bronchial collapse). The clinical presentation of AC may overlap with non-collapsible airway disease (NCAD) or another non-lower airway respiratory disease (NLARD). This study determined whether paired inspiratory (I)/expiratory (E)-breath-hold computed tomography (I/E-BH CT) can detect a static and dynamic AC in dogs with spontaneous respiratory disease and it compared the CT-derived metrics of the AC to the tracheobronchoscopy metrics. The CT-acquired I and E diameter and cross-sectional area (CSA) for the trachea, mainstem and lobar bronchi in dogs with an AC (n = 16), NCAD (16), and NLARD (19) served for a dynamic percent of the airway narrowing (%AN) calculation. A scoring system assessed the bronchomalacia. The circularity was calculated for each airway. The results were compared to the tracheobronchoscopy collapse grading. In the dogs with an AC, the %AN was larger for the trachea, right mainstem bronchus and right middle lobar bronchus when they were compared to the dogs with NCAD and NLARD. Flattening was only identified for the trachea of the AC dogs. The agreement between the CT and tracheobronchoscopy scores was 20% from trachea to the lobar bronchi and 47% for the segmental/subsegmental bronchi. Paired I/E-BH CT can detect static and dynamic AC with limited agreement with the tracheobronchoscopy metrics. Independent scoring systems that are tailored to the clinical manifestations of functional impairments are needed.
Effect of Hay Steaming on the Estimated Precaecal Digestibility of Crude Protein and Selected Amino Acids in Horses
Authors: Pisch Caroline , Wensch-Dorendorf Monika , Schwarzenbolz Uwe , Henle Thomas , Greef Jörg Michael , Zeyner Annette
Published: November 2022

Steaming hay is increasingly used to treat low-quality forage because it was proven to reduce inhalable allergens such as mould spores, bacteria, and airborne dust particles. Preliminary results have shown a substantial loss of precaecal (pc) digestibility (D) of crude protein (CP) and amino acids (AA). For this purpose, six different batches of hay from central Germany were divided into four subsamples, and each one was individually steamed. Native hay and four replicates of each steamed subsample were analysed for CP, AA, neutral detergent insoluble crude protein (NDICP), neutral detergent soluble crude protein (NDSCP) as well as pepsin insoluble CP (piCP). Based on the analytical parameters, pcD of CP, protein solubility (PS), piCP (% CP) and precaecal digestible (pcd) CP and pcdAA contents were calculated. Selected Maillard reaction products (MRP), namely furosine and carboxymethyllysine (CML), were also analysed. Steaming did not affect CP content (native = 69, steamed = 67 g/kg dry matter, DM; p > 0.05), but it had an impact on the insoluble part of CP. Thus, NDICP increased by 57% (native = 27, steamed = 42 g/kg DM; p < 0.05) and piCP by 15% overall (native = 40, steamed = 46% of CP; p < 0.05). This could be a consequence of the heat damage and the associated increase in MRP. The content of furosine rose by 67% (native = 17.6, steamed = 29.4 mg/100 g DM; p < 0.05). The content of CML increased by 120% (native = 5.1, steamed = 11.3 mg/100 g DM; p < 0.05). We chose to analyse these two MRPs because they represent the reaction products with the limiting AA lysine. In contrast, the soluble fractions of CP declined, while PS as a percentage of CP decreased by 38% as a result of the treatment, and NDSCP was reduced by as much as 41% (p < 0.05). In line with this, the steaming process decreased the pcD of CP (native = 56%, steamed = 35%; p < 0.05) and pcdCP (native = 37.9, steamed = 22.5 g/kg DM; p < 0.05), respectively. The same effects were shown for selected AA; e.g., sulphuric AA pcd methionine plus pcd cysteine decreased by 45%, pcd threonine decreased by 41%, and the limited AA pcd lysine decreased by more than 50% (p < 0.05). In conclusion, the high temperatures generated during steaming lead to protein damage and consequently to a reduction in the pcD of CP and essential AA. Nevertheless, steaming successfully reduces viable microorganisms and binds dust particles. Therefore, steamed hay is still a proper and sometimes the only possible roughage for horses suffering from respiratory diseases such as equine asthma. Essentially, horse diets based on steamed hay should be balanced accordingly.
Prevalence of Antibiotic Prescription in Patients with Acute Rhinosinusitis Treated by General Practitioners and Otolaryngologists in Germany—A Retrospective Cohort Study
Authors: Bittner Claudia B. , Plach Michael , Steindl Hubert , Abramov-Sommariva Dimitri , Abels Christoph , Kostev Karel
Published: November 2022

(1) Background: The goal of this retrospective cohort study, based on real-world data and conducted in Germany, was to investigate the prevalence of antibiotic (AB) prescription in patients with acute rhinosinusitis (ARS). (2) Methods: Data from the Disease Analyzer database were used for this cross-sectional study. Patients aged ≥18 years diagnosed with acute sinusitis by general practitioners (GPs) and ear, nose, throat (ENT) specialists between January 2012 and December 2020 were included. The main outcome of the study was the proportion of patients with ARS who received an AB prescription on the day of diagnosis or within three days afterwards. The proportion was estimated separately for patients treated by GPs and ENTs, and also for five age groups, as well as women and men. (3) Results: In total, 308,095 patients were diagnosed with ARS (187,838 by GPs and 120,257 by ENTs). 50.9% of patients treated by GPs and 50.0% treated by ENTs received an AB prescription. AB prevalence increased with age from 46.9% in the age group 18–30 years to 55.5% in the age group > 60 years. (4) Conclusions: We have shown a high prevalence of potentially inappropriate AB prescription for adult patients with ARS in both GP and ENT practices and also among both women and men and in several age groups. There is an urgent need for interventions to reduce inappropriate AB use.
Prevalence, Tetracycline Resistance and Tet(O) Gene Identification in Pathogenic Campylobacter Strains Isolated from Chickens in Retail Markets of Lima, Peru
Authors: Benites Christian , Anampa Diego , Torres Domingo , Avalos Ivette , Rojas Miguel , Conte Carlos , Lázaro César
Published: November 2022

Background: In this study, we aimed to estimate the prevalence, tetracycline resistance and presence of Tet(O) in Campylobacter strains isolated from chicken in markets of Lima, Peru. Methods: A total of 250 chicken samples were obtained from traditional markets (skin, n = 120) and supermarkets (meat, n = 130). Samples were subjected to microbiological assays for identification of Campylobacter spp. according to ISO 10272-2017, and the isolates were then submitted to species identification by PCR. Phenotypic resistance to tetracyclines was assessed by the Kirby–Bauer test, and the presence of the Tet(O) gene was determined by PCR. Results: A significantly higher prevalence (p < 0.0001) of Campylobacter coli in skin samples from traditional markets (97.5%) than in meat samples from supermarkets (36.2%) was observed. On the other hand, Campylobacter jejuni was confirmed only in 3.1% of meat samples. All Campylobacter species isolated from skin and meat samples were phenotypically resistant to tetracyclines; however, the presence of the Tet(O) gene in C. coli was identified in 76.9% and 66.0% of skin and meat samples, no significant statistical difference (p = 0.1488) was found between these prevalence. All C. jejuni isolated from chicken meat samples from supermarkets were positive for Tet(O) gene. Conclusions: This study confirms the high prevalence of C. coli isolated from chicken sold in traditional markets and supermarkets in Lima, Peru, and in more than 70% of these strains, phenotypic resistance to tetracyclines could be linked with expression of the Tet(O) gene. It is necessary to evaluate other genes involved in resistance to tetracyclines and other groups of antibiotics in campylobacter strains isolated from chicken meat.
Evolution of the Concept of Sepsis
Authors: Vincent Jean-Louis
Published: November 2022

Sepsis has been recognized for more than 2500 years, but the criteria used to identify it have evolved. Sepsis is an infection associated with some degree of organ dysfunction—put very simplistically, sepsis is a ‘bad infection’. Specific criteria may be useful for research purposes but have less value in day-to-day clinical practice. What is relevant here is early recognition and some awareness of severity so that appropriate therapy can be started without delay.
Population Pharmacokinetics of Prophylactic Cefazolin in Cardiac Surgery with Standard and Minimally Invasive Extracorporeal Circulation
Authors: Šantavý Petr , Šíma Martin , Zuščich Ondřej , Kubíčková Vendula , Michaličková Danica , Slanař Ondřej , Urbánek Karel
Published: November 2022

The objectives of this study were to develop a population pharmacokinetic model of prophylactically administered cefazolin in patients undergoing cardiac surgery with and without the use of the cardiopulmonary bypass of both existing types—standard (ECC) and minimallyu invasive extracorporeal circulation (MiECC)—and to propose cefazoline dosing optimization based on this model. A total of 65 adult patients undergoing cardiac surgery were recruited to this clinical trial. A prophylactic cefazolin dose of 2 g was intravenously administered before surgery. Blood samples were collected using a rich sampling design and cefazolin serum concentrations were measured using the HPLC/UV method. The pharmacokinetic population model was calculated using a nonlinear mixed-effects modeling approach, and the Monte Carlo simulation was used to evaluate the PK/PD target attainment. The population cefazolin central volume of distribution (Vd) of 4.91 L increased by 0.51 L with each 1 m2 of BSA, peripheral Vd of 22.07 L was reduced by 0.77 L or 0.79 L when using ECC or MiECC support, respectively, while clearance started at 0.045 L/h and increased by 0.49 L/h with each 1 mL/min/1.73 m2 of eGFR. ECC/MiECC was shown to be covariate of cefazolin Vd, but without relevance to clinical practice, while eGFR was most influential for the PK/PD target attainment. The standard dose of 2 g was sufficient for PK/PD target attainment throughout surgery in patients with normal renal status or with renal impairment. In patients with augmented renal clearance, an additive cefazolin dose should be administered 215, 245, 288 and 318 min after the first dose at MIC of 4, 3, 2 and 1.5 mg/L, respectively.
Controlling of Bacterial Virulence: Evaluation of Anti-Virulence Activities of Prazosin against Salmonella enterica
Authors: Elfaky Mahmoud A. , Thabit Abrar K. , Eljaaly Khalid , Zawawi Ayat , Abdelkhalek Ahmed S. , Almalki Ahmad J. , Ibrahim Tarek S. , Hegazy Wael A. H.
Published: November 2022

Salmonella enterica is a Gram-negative orofecal transmitted pathogen that causes a wide diversity of local and systemic illnesses. Salmonella enterica utilizes several interplayed systems to regulate its invasion and pathogenesis: namely, quorum sensing (QS) and type three secretion system (T3SS). In addition, S. enterica could sense the adrenergic hormones in the surroundings that enhance its virulence. The current study aimed to evaluate the ability of α-adrenoreceptor antagonist prazosin to mitigate the virulence of S. enterica serovar Typhimurium. The prazosin effect on biofilm formation and the expression of sdiA, qseC, qseE, and T3SS-type II encoding genes was evaluated. Furthermore, the prazosin intracellular replication inside macrophage and anti-virulence activity was evaluated in vivo against S. typhimurium. The current finding showed a marked prazosin ability to compete on SdiA and QseC and downregulate their encoding genes. Prazosin significantly downregulated the virulence factors encoding genes and diminished the biofilm formation, intracellular replication inside macrophages, and in vivo protected mice. To sum up, prazosin showed significant inhibitory activities against QS, T3SS, and bacterial espionage, which documents its considered anti-virulence activities.
Furaquinocins K and L: Novel Naphthoquinone-Based Meroterpenoids from Streptomyces sp. Je 1-369
Authors: Tistechok Stepan , Stierhof Marc , Myronovskyi Maksym , Zapp Josef , Gromyko Oleksandr , Luzhetskyy Andriy
Published: November 2022

Actinomycetes are the most prominent group of microorganisms that produce biologically active compounds. Among them, special attention is focused on bacteria in the genus Streptomyces. Streptomycetes are an important source of biologically active natural compounds that could be considered therapeutic agents. In this study, we described the identification, purification, and structure elucidation of two new naphthoquinone-based meroterpenoids, furaquinocins K and L, from Streptomyces sp. Je 1-369 strain, which was isolated from the rhizosphere soil of Juniperus excelsa (Bieb.). The main difference between furaquinocins K and L and the described furaquinocins was a modification in the polyketide naphthoquinone skeleton. In addition, the structure of furaquinocin L contained an acetylhydrazone fragment, which is quite rare for natural compounds. We also identified a furaquinocin biosynthetic gene cluster in the Je 1-369 strain, which showed similarity (60%) with the furaquinocin B biosynthetic gene cluster from Streptomyces sp. KO-3988. Furaquinocin L showed activity against Gram-positive bacteria without cytotoxic effects.
Development of Intestinal Injury and Restoration of Weaned Piglets under Chronic Immune Stress
Authors: Yu Jiayi , Zheng Changbing , Zheng Jie , Duan Geyan , Guo Qiuping , Zhang Peiwen , Wan Mengliao , Duan Yehui
Published: November 2022

This study aimed to investigate the effects of lipopolysaccharide (LPS)-induced chronic immune stress on intestinal morphology and function, immune system, oxidative status, and mitochondrial function in piglets. Fifty healthy Duroc × Landrace × Yorkshire piglets (21 ± 2 days old, barrow, 6.98 ± 0.14 kg body weight) were selected and randomly allotted to five groups, which were slaughtered at 0 (0 group), 1, 5, 9, and 15 d of LPS injection. The results showed that compared with the piglets without LPS injection, LPS injection significantly impaired the intestinal morphology and permeability at 1, 5, and 9 d, as manifested by the increased serum lactic acid and decreased ratio of villus height to crypt depth (p < 0.05). Moreover, intestinal inflammation and oxidative and mitochondrial injury were caused at 1 d, as manifested by upregulated IL-6 mRNA expression, increased malondialdehyde content, and impaired mitochondrial morphology (p < 0.05). However, these parameters were restored to levels identical to 0 group at 9~15 d, accompanied by significantly increased antioxidant capacity, enhanced protein expression of CD3+ and CD68+, and upregulated mRNA abundance of genes related to mitochondrial biogenesis and functions (p < 0.05). Collectively, these results suggest that the intestinal injury of piglets caused by chronic immune stress could be self-repaired.
Role of Oxidative Stress and Lipid Peroxidation in the Pathophysiology of NAFLD
Authors: Martín-Fernández Marta , Arroyo Víctor , Carnicero Carmen , Sigüenza Rebeca , Busta Reyes , Mora Natalia , Antolín Beatriz , Tamayo Eduardo , Aspichueta Patricia , Carnicero-Frutos Irene , Gonzalo-Benito Hugo , Aller Rocío
Published: November 2022

Non-alcoholic fatty liver disease (NAFLD) is characterised by an excess of hepatic fat that can progress to steatohepatitis, fibrosis, cirrhosis and hepatocarcinoma. The imbalance between lipid uptake/lipogenesis and lipid oxidation/secretion in the liver is a major feature of NAFLD. Given the lack of a non-invasive and reliable methods for the diagnosis of non-alcoholic steatohepatitis (NASH), it is important to find serum markers that are capable of discriminating or defining patients with this stage of NASH. Blood samples were obtained from 152 Caucasian subjects with biopsy-proven NAFLD due to persistently elevated liver enzyme levels. Metabolites representative of oxidative stress were assessed. The findings derived from this work revealed that NAFLD patients with a NASH score of ≥ 4 showed significantly higher levels of lipid peroxidation (LPO). Indeed, LPO levels above the optimal operating point (OOP) of 315.39 μM are an independent risk factor for presenting a NASH score of ≥ 4 (OR: 4.71; 95% CI: 1.68–13.19; p = 0.003). The area under the curve (AUC = 0.81, 95% CI = 0.73–0.89, p < 0.001) shows a good discrimination ability of the model. Therefore, understanding the molecular mechanisms underlying the basal inflammation present in these patients is postulated as a possible source of biomarkers and therapeutic targets in NASH.
Identification and Quantification of Key Phytochemicals, Phytohormones, and Antioxidant Properties in Coccinia grandis during Fruit Ripening
Authors: Lee In Young , Joo Nami
Published: November 2022

Coccinia grandis contains secondary metabolites, such as flavonoids, phenolic acids, terpenoids, alkaloids, sterols, and glycosides, which are known to have in vitro antioxidant, antidiabetic, anti-inflammatory, and antidyslipidemic activities. C. grandis fruits change dramatically during ripening, and the differences in the phytochemicals contribute to various uses. This study reports the phytochemical compounds and antioxidant activities during ripening of C. grandis for the first time. Characterizations were conducted on the physiologically active substances in C. grandis fruits at three ripening stages, and a total of 25 peaks were identified. Key phytochemicals in the ripening stages of C. grandis were identified, and the major substances that contributed to antioxidant properties were selected and quantitatively analyzed. Although the concentration of tiliroside increased during aging, hydroxycinnamic acid (chlorogenic and p-coumaric acids), flavonols (rutin), and triterpenes (cucurbitacins B and D) with antioxidant effects decreased. Therefore, phenolic compounds and cucurbitacins dominate immature C. grandis quantitatively. Regarding phytohormones, the gibberellin A4 content decreased as the fruits matured, but indoleacetic acid and salicylic acid increased with fruit maturity. The antioxidant capacities determined by DPPH and ABTS consistently decreased with increasing maturity. Accordingly, the extracts of immature C. grandis fruits have high levels of bioactive compounds and can be used to develop food additives and health supplements.
Experimental Study on the Straw Flexible Space Assembly Flood Control System
Authors: Lin Li , Shen Yuhang , Yuan Xin , Yao Yujian , Xu Tianli
Published: November 2022

By using straw to build an assembled flood wall, this study provided new ideas for useful applications of straw and for the development of assembly flood control facilities. A straw flexible space assembly flood control system is designed on the basis of Revit, and a hydraulic test of a scaled-down model of the system is conducted using a water-retaining test machine to prove the stability and impact resistance of the system. Finally, a numerical analysis model of the straw flexible space flood control system is established using ABAQUS software to perform finite element analysis of the system. A comparison of the test results and the numerical analysis results shows that they can be well fitted. Thus, this study lays a theoretical foundation for a large-scale engineering application of the proposed system.
Nonlinear Model Predictive Control with Terminal Cost for Autonomous Vehicles Trajectory Follow
Authors: Nan Jinrui , Ye Xucheng , Cao Wanke
Published: November 2022

This paper presents a nonlinear model predictive control with terminal cost (NMPC–WTC) algorithm and its open/closed-loop system analysis and simulation validation for accurate and stable path tracking of autonomous vehicles. The path tracking issue is formulated as an optimal control problem. In order to improve the squeezing phenomenon of traditional NMPC, a discrete-time nonlinear model predictive controller with terminal cost is then designed, in which the state error of last step is augmented. The cost function of NMPC–WTC consists of two parts: (1) the traditional NMPC cost function responding to tracking errors and controller output, and (2) the augmented terminal cost. The algorithm was implemented on CasADi numerical optimization framework, which is free, open-source and developed for nonlinear optimization. The open-loop and closed-loop simulation results are then presented to demonstrate the improved performance in tracking accuracy and stability compared to traditional model predictive controller.
Experimental Study on the Permeability of Rare Earths with Different Particle Composition for a Novel Heap Leaching Technology
Authors: Ju Wei , Yang Jianhua , Yao Chi , Zhang Xiaobo , Ye Zhiwei , Liu Da
Published: November 2022

In order to solve the problem of vegetation restoration in the traditional heap leaching of rare earths, a novel heap leaching technology is proposed. In the novel technology, leaching liquid is laterally injected into the rare earth pile from the side end so that vegetation restoration can be carried out quickly on the top of the pile. In this study, a laboratory test was performed to investigate the permeability of the ion-adsorbed rare earth pile under lateral liquid injection conditions. Four rare earth samples with varied combinations of the mineral particles at different sizes were adopted in the test to emphatically discuss the influence of the particle composition on permeability. The experimental results show that the permeability of the rare earth pile under the lateral liquid injection conditions is governed by the migration of fine particles. As the hydraulic head of the leaching liquid increases, the fine particles undergo a motion process of stabilization, migration initiation, deposition, and remigration. Accordingly, the permeability coefficient of the rare earth pile exhibits a variation of stabilization, a gradual increase, a re-stabilization, and a re-increase. The fine particle migration and exudation causes the permeability of the rare earth pile to be non-uniform in space. The permeability coefficients near the liquid injection end and the liquid outlet end are much greater than those are at the middle positions of the pile. The particle composition of the rare earth has an important effect on the permeability of the pile. In the rare earth with a combination of coarse and fine particles, preferential flow paths are easy to develop as in this soil, the fine particles easily migrate and they cannot block the pore channels. By contrast, the fine particles find it difficult to migrate, and the permeability coefficient is relatively stable in the fine-medium particle rare earth. This particle composition is conducive to the heap leaching of the rare earth under lateral liquid injection conditions.
Complex Mathematical Modeling of the Well Drilling Process
Authors: Churilova Maria , Lupuleac Sergey , Shaposhnikov Nikita
Published: November 2022

Recently, the technologies for the global modeling of the process of oil well drilling have become widespread. Mathematical modeling is used in well design, virtual testing of various drilling equipment, simulations of various emergency situations, and personnel training. Complex modeling of the well drilling process includes the simulation of such phenomena as the dynamics of the drill string and its contact interaction with walls, the flow of the drilling fluid and its interaction with the soil (considering influxes and leakages), soil crushing by the drill, the transfer of cuttings particles by the drilling fluid, heat exchange with the soil, and others. This paper provides a detailed review of the existing modeling approaches to solving such problems. Most of the studies included in the review focus on building a detailed mathematical model of one or several of the above processes. Moreover, all these processes mutually influence each other, which also needs to be considered in the analysis. It appears that further development of such a multiphysics approach will be the main direction of research in this area in the near future.
Towards Watt-Level THz Sources for High-Resolution Spectroscopy Based on 5th-Harmonic Multiplication in Gyrotrons
Authors: Denisov Grigory , Zotova Irina , Zheleznov Ilya , Malkin Andrey , Sergeev Alexander , Rozental Roman , Glyavin Mikhail
Published: November 2022

We propose the concept of high-power THz radiation sources based on five-fold frequency multiplication in gyrotrons intended for plasma applications. The efficient excitation at the 5th cyclotron harmonic is due to the specific property of the eigenmodes of cylindrical waveguides, as a result of which, the conditions of simultaneous electrodynamic resonance at two selected TE modes are satisfied asymptotically with very high accuracy. Previously, we have verified this principle in experiments with a low-frequency kilowatt-level gyrotron in which, due to the low-density spectrum, the operating mode is excited with no competition from parasitic oscillations. The novel concept is a development of this idea applied to the systems with a denser spectrum, which is inevitable in higher frequency and power devices. Simulations within the averaged time-domain model demonstrate that, despite the mode competition, it is possible to excite Watt-level 1.25 THz 5th cyclotron harmonic in a recently developed sub-MW 0.25 THz gyrotron with TE19,8 operating mode. The obtained results open a possibility for implementation of radiation sources with output power/frequency combination, practically inaccessible using other THz generation methods and highly sought for a number of applications, including high-resolution molecular spectroscopy.
SEM Evaluation of the Hybrid Layer of Two Universal Adhesives on Sound and DI Type II Affected Dentin
Authors: Libonati Antonio , Di Taranto Virginia , Memè Lucia , Gallusi Gianni , Campanella Vincenzo
Published: November 2022

Universal nanofilled adhesives were recently introduced in restorative dentistry to simplify clinical procedures and improve adhesion in different clinical situation. This study investigated the effectiveness of two universal adhesives on both sound and dentinogenesis imperfecta type II (DI-II)-affected teeth. To evaluate the effectiveness of adhesion on both sound and DI-II-affected teeth, four samples, two sound extracted molars and two extracted molars affected by DI-II were selected. Coronal enamel and dentin were exposed, and the samples were used for testing two different universal adhesives, Universal Bond (Tokuyama) and All-Bond Universal (Bisco). After the adhesive procedures, the samples were stored in saline at room temperature for one week prior to SEM investigation for the interfacial bonding layer. The samples were longitudinally sectioned into two parts, obtaining two sections for the evaluation of the adhesive interface to the SEM. The SEM-morphology of the hybrid layer on the enamel was similar for the two universal adhesives tested. The study of the hybrid layer on sound dentin confirmed the great versatility of All-Bond Universal and Universal Bond adhesives in managing adhesion even on pathological dental substrates. Both universal adhesives tested showed encouraging results on DI-II-affected dentin, creating an effective hybrid layer even on the atubular and less mineralized altered dentin.
Impact of Image Preprocessing Methods and Deep Learning Models for Classifying Histopathological Breast Cancer Images
Authors: Murcia-Gómez David , Rojas-Valenzuela Ignacio , Valenzuela Olga
Published: November 2022

Early diagnosis of cancer is very important as it significantly increases the chances of appropriate treatment and survival. To this end, Deep Learning models are increasingly used in the classification and segmentation of histopathological images, as they obtain high accuracy index and can help specialists. In most cases, images need to be preprocessed for these models to work correctly. In this paper, a comparative study of different preprocessing methods and deep learning models for a set of breast cancer images is presented. For this purpose, the statistical test ANOVA with data obtained from the performance of five different deep learning models is analyzed. An important conclusion from this test can be obtained; from the point of view of the accuracy of the system, the main repercussion is the deep learning models used, however, the filter used for the preprocessing of the image, has no statistical significance for the behavior of the system.
Experimental Investigation of Task Performance and Human Vigilance in Different Noise Environments
Authors: Yang Chenyuan , Pang Liping , Liang Jin , Cao Xiaodong , Fan Yurong , Zhang Jie
Published: November 2022

Twelve healthy male college-age students were recruited to investigate the effects of different noise exposure conditions on complex task performance and vigilance. During each noise exposure, the Multi-Attribute Task Battery (MATB) with low, medium, and high mental workloads were conducted in the order designated by the Latin square method. Meanwhile, a psychomotor vigilance test (PVT) was used to evaluate human vigilance. Heart rate variability (HRV) signals were also collected while participants performed the tasks. The generalized additive mixed-effect model (GAMM) results showed that the increased mental workload had an inverted U-shaped effect on MATB task performance. Noise exposure had no significant impact on the overall performance of MATB tasks. However, when exposed to increased noise sharpness at low mental workloads, Tracking Task (TRA) performance significantly decreased, whereas the System Monitoring Task (SYS) performance was significantly improved. In addition, higher noise sound pressure level and sharpness would impair human vigilance, which was reflected in a lower mean sample entropy of HRV and worse performance on the PVT. The results indicated that noise control in the workplace should consider both sound pressure level and sharpness.
On the Residual Static and Impact Capacity of Shear-Reinforced Concrete Beams Subjected to an Initial Impact
Authors: Peterson Viktor , Ansell Anders , Hallgren Mikael
Published: November 2022

Impact loads in previous research showed to induce brittle responses of statically flexure-critical reinforced concrete (RC) beams designed for ductility. The impact load may produce flexural shear damage modes similar to that observed during quasi-static loads and local shear damage under the impact zone. The occurrence of shear damage modes during impact tests has been investigated extensively, but their effect on the residual quasi-static and dynamic capacity is not fully understood. For this aim, an initial high-velocity impact test initiated severe shear damage to RC beams. The beams were then tested quasi-statically and by sequential impact testing using the same setup as the initial tests. The results indicate a flexure-dominated response during sequential impact tests for beams containing extreme shear reinforcement amounts, favouring the energy-absorption capacity. Significant shear and flexural damage occurred for beams with less shear reinforcement, indicating a hybrid response that varied throughout the tests. The tests for the residual quasi-static capacity indicated severe consequences from initial local shear damage on the capacity, as shown by the brittle response of the beam with the most shear reinforcement. However, wide initial flexural cracks instead showed a favourable effect, as there was an indication of transfer from brittle to ductile failure. For beams showing both global and local shear damage, it was concluded that global shear damage modes were critical for the residual static and dynamic shear capacity.
Are the Current Research Methods Reliable for Evaluating the Mechanical Performance of NiTi Endodontic Rotary Instruments?
Authors: Di Russo Franco Maria , Gisario Annamaria , Natali Stefano , Bellanova Valentina , Leone Chiara , Testarelli Luca
Published: November 2022

Technological innovation and the modernization of manufacturing procedures have thoroughly redefined the field of nickel–titanium (NiTi) rotary and reciprocating endodontic instruments [...]
Granular Description of Uncertain Data for Classification Rules in Three-Way Decision
Authors: Zhang Xinhui , Ouyang Tinghui
Published: November 2022

Considering that data quality and model confidence bring threats to the confidence of decision-making, a three-way decision with uncertain data description is more meaningful in system analyses. In this paper, an advanced method for forming classification rules in three-way decisions is proposed. This method firstly constructs information granules for describing uncertain data in decision-making; meanwhile, information entropy is introduced in Granular Computing (GrC) to realize a better uncertainty description. Then, based on the constructed uncertainty descriptors, fuzzy rules are formed aiming at the common decision-making processes, namely classification problems. Finally, experiments on both synthetic and publicly available data are implemented. Discussions on numerical results validate the feasibility of the proposed method for forming three-way classification rules. Moreover, classification rules with consideration of uncertain data are demonstrated to be better performed than traditional methods with an improvement of 1.35–4.26% in decision-making processes.
Aerobic Upper-Limb Exercise-Induced Hypoalgesia: Does It Work?
Authors: Katz-Betzalel Noa , Weissman-Fogel Irit , Kodesh Einat
Published: November 2022

Background: Aerobic exercise reduces pain sensitivity, a phenomenon known as exercise-induced hypoalgesia (EIH); however, little is known about EIH when the upper limbs are aerobically exercised. This study aimed to test the acute effect of a single aerobic upper-limb exercise on pain threshold and pain intensity in healthy participants, with two different protocols for controlling intensity. Methods: 31 participants performed two 20 min exercise sessions a week apart. In each session, the intensity was controlled by a target heart rate (THR) of 60% of heart rate reserve or by a rate of perceived exertion (RPE) of 7/10 on the Borg scale. Pain threshold for pressure (PPT) heat (HPT) and pain intensity in response to Tonic Heat Pain (THP) were measured pre- and post-exercise. To examine the effect of exercise in each protocol on pain sensitivity, rmANOVA was conducted. Results: Pain sensitivity remained unchanged following arm exercise in both protocols (PPT, p = 0.67; HPT, p = 0.56; and THP p = 0.39). Higher HR in the THR protocol was demonstrated with a significant protocol X time, interaction effect (F(3) = 11.194 p < 0.004). Conclusions: Moderate–high-intensity upper-limb aerobic exercise did not affect pain sensitivity in healthy individuals. Exercise intensity when controlled by THR showed a higher mean heart rate compared to exercise intensity based on RPE.