bluuebunny/crossref_metadata_2025 huggingface dataset:
Practical Applications of Molecular Testing in the Cytologic Diagnosis of Pancreatic Cysts
Authors: Zhang Mingjuan Lisa , Pitman Martha B.
Published: February 2021

Mucinous pancreatic cysts are precursor lesions of ductal adenocarcinoma. Discoveries of the molecular alterations detectable in pancreatic cyst fluid (PCF) that help to define a mucinous cyst and its risk for malignancy have led to more routine molecular testing in the preoperative evaluation of these cysts. The differential diagnosis of pancreatic cysts is broad and ranges from non-neoplastic to premalignant to malignant cysts. Not all pancreatic cysts—including mucinous cysts—require surgical intervention, and it is the preoperative evaluation with imaging and PCF analysis that determines patient management. PCF analysis includes biochemical and molecular analysis, both of which are ancillary studies that add significant value to the final cytological diagnosis. While testing PCF for carcinoembryonic antigen (CEA) is a very specific test for a mucinous etiology, many mucinous cysts do not have an elevated CEA. In these cases, detection of a KRAS and/or GNAS mutation is highly specific for a mucinous etiology, with GNAS mutations supporting an intraductal papillary mucinous neoplasm. Late mutations in the progression to malignancy such as those found in TP53, p16/CDKN2A, and/or SMAD4 support a high-risk lesion. This review highlights PCF triage and analysis of pancreatic cysts for optimal cytological diagnosis.
Broadband Sound Intensity Interference Frequency Periodicity and Pulse Source Localization
Authors: Zhang Shizhao , Piao Shengchun
Published: February 2021

In order to analyze the frequency periodicity characteristics of acoustic field interference and realize acoustic source ranging (ASR), the normal mode model is used to analyze the interference characteristics of the broadband acoustic field under the condition of horizontally layered medium; the broadband received signal field when the broadband pulse signal passes through the acoustic field is also simulated. The variation of interference patterns with frequency is analyzed, and their spatial interference characteristics and mechanisms are analyzed. Based on the interference theory, the relation between the acoustic source range and the frequency periodicity of the broadband acoustic intensity interference is derived. Simulation and experimental results show that this relation can accurately estimate the far-field acoustic source range, and the estimation accuracy and real-time performance are greatly improved compared with previous methods. Besides, simulation shows that the method combined with multiple-receiver ranging obtains high-precision direction of arrival (DOA) estimation as well as ASR. The relation between acoustic source position and broadband acoustic field interference frequency periodicity can be used to improve far-field ASR and DOA estimation, which is of great value for oceanography, marine engineering, and marine military. In addition, this relation can also be extended to that between the modal interference frequency periodicity and other related parameters in other physical fields for parameter inversion.
As-Built Simulation of the High Flux Isotope Reactor
Authors: Betzler Benjamin R. , Chandler David , Evans Thomas M. , Davidson Gregory G. , Daily Charles R. , Wilson Stephen C. , Mosher Scott W.
Published: February 2021

The Oak Ridge National Laboratory High Flux Isotope Reactor (HFIR) is an 85 MWt flux trap-type research reactor that supports key research missions, including isotope production, materials irradiation, and neutron scattering. The core consists of an inner and an outer fuel element containing 171 and 369 involute-shaped plates, respectively. The thin fuel plates consist of a U3O8-Al dispersion fuel (highly enriched), an aluminum-based filler, and aluminum cladding. The fuel meat thickness is varied across the width of the involute plate to reduce thermal flux peaks at the radial edges of the fuel elements. Some deviation from the designed fuel meat shaping is allowed during manufacturing. A homogeneity scan of each fuel plate checks for potential anomalies in the fuel distribution by scanning the surface of the plate and comparing the attenuation of the beam to calibration standards. While typical HFIR simulations use homogenized fuel regions, explicit models of the plates were developed under the Low-Enriched Uranium Conversion Program. These explicit models typically include one inner and one outer fuel plate with nominal fuel distributions, and then the plates are duplicated to fill the space of the corresponding fuel element. Therefore, data extracted from these simulations are limited to azimuthally averaged quantities. To determine the reactivity and physics impacts of an as-built outer fuel element and generate azimuthally dependent data in the element, 369 unique fuel plate models were generated and positioned. This model generates the three-dimensional (i.e., radial–axial–azimuthal) plate power profile, where the azimuthal profile is impacted by features within the adjacent control element region and beryllium reflector. For an as-built model of the outer fuel element, plate-specific homogeneity data, 235U loading, enrichment, and channel thickness measurements were translated into the model, yielding a much more varied azimuthal power profile encompassed by uncertainty factors in analyses. These models were run with the ORNL-TN and Shift Monte Carlo tools, and they contained upwards of 500,000 cells and 100,000 unique tallies.
The Mechanical Impact of Water Affected the Soil Physical Quality of a Loam Soil under Minimum Tillage and No-Tillage: An Assessment Using Beerkan Multi-Height Runs and BEST-Procedure
Authors: Castellini Mirko , Stellacci Anna Maria , Sisto Danilo , Iovino Massimo
Published: February 2021

The multi-height (low, L = 3 cm; intermediate, M = 100 cm; high, H = 200 cm) Beerkan run methodology was applied on both a minimum tilled (MT) (i.e., up to a depth of 30 cm) and a no-tilled (NT) bare loam soil, and the soil water retention curve was estimated by the BEST-steady algorithm. Three indicators of soil physical quality (SPQ), i.e., macroporosity (Pmac), air capacity (AC) and relative field capacity (RFC) were calculated to assess the impact of water pouring height under alternative soil management practices. Results showed that, compared to the reference low run, M and H runs affected both the estimated soil water retention curves and derived SPQ indicators. Generally, M–H runs significantly reduced the mean values of Pmac and AC and increased RFC for both MT and NT soil management practices. According to the guidelines for assessment of SPQ, the M and H runs: (i) worsened Pmac classification of both MT and NT soils; (ii) did not worsen AC classification, regardless of soil management parameters; (iii) worsened RFC classification of only NT soil, as a consequence of insufficient soil aeration. For both soil management techniques, a strong negative correlation was found between the Pmac and AC values and the gravitational potential energy, Ep, of the water used for the infiltration runs. A positive correlation was detected between RFC and Ep. The relationships were plausible from a soil physics point of view. NT soil has proven to be more resilient than MT. This study contributes toward testing simple and robust methods capable of quantifying soil degradation effects, due to intense rainfall events, under different soil management practices in the Mediterranean environment.
Integrating Microclimate into Landscape Architecture for Outdoor Thermal Comfort: A Systematic Review
Authors: Lin Jiawei , Brown Robert
Published: February 2021

Global climate change and urban heat island intensification are making many cities dangerously hot during heat waves and uncomfortably hot much of the time. Research has identified ways that urban environments can be designed to reduce the heat, but much of the information is too technical or has not been interpreted or communicated so as to be available to landscape architects. This study identifies ways that landscape architecture researchers have applied microclimate information in design to proactively create more thermally comfortable outdoor environments. A systematic review that assessed the growing recognition of microclimatic factors in design revealed four main approaches: principles and guidelines, strategies, mapping, and evaluation. The advantages and limitations of each have been noted, and a diagram has been developed that matches each approach with specific steps in the landscape architectural design process. The study also identified four areas where microclimate has potential for use in landscape architecture but that are currently not being very actively studied: education, modeling and visualization, policy, and ideation. Microclimatic design has the potential to enhance the health and well-being of the public through the design of thermally comfortable outdoor environments.
Rolling Cargo Management Using a Deep Reinforcement Learning Approach
Authors: Oucheikh Rachid , Löfström Tuwe , Ahlberg Ernst , Carlsson Lars
Published: February 2021

Loading and unloading rolling cargo in roll-on/roll-off are important and very recurrent operations in maritime logistics. In this paper, we apply state-of-the-art deep reinforcement learning algorithms to automate these operations in a complex and real environment. The objective is to teach an autonomous tug master to manage rolling cargo and perform loading and unloading operations while avoiding collisions with static and dynamic obstacles along the way. The artificial intelligence agent, representing the tug master, is trained and evaluated in a challenging environment based on the Unity3D learning framework, called the ML-Agents, and using proximal policy optimization. The agent is equipped with sensors for obstacle detection and is provided with real-time feedback from the environment thanks to its own reward function, allowing it to dynamically adapt its policies and navigation strategy. The performance evaluation shows that by choosing appropriate hyperparameters, the agents can successfully learn all required operations including lane-following, obstacle avoidance, and rolling cargo placement. This study also demonstrates the potential of intelligent autonomous systems to improve the performance and service quality of maritime transport.
New Composite Material for Masonry Repair: Mortar Formulations and Experimental Studies
Authors: Deboucha Walid , Alachek Ibrahim , Plassiard Jean-Patrick , Plé Olivier
Published: February 2021

The need for retrofitting existing masonry structures is progressively becoming more important due to their continuous deterioration or need to meet the current design requirements of Eurocodes. Textile-Reinforced Mortar (TRM) composite systems have emerged as a sustainable repair methodology suitable for structure retrofitting. Nevertheless, their mechanical performance is still far from being fully investigated. This paper presents an experimental study on the tensile and bond behaviors of a new mortar-based composite consisting of mineral additives, blended cement mortar, and stainless-steel grid. Three different mineral additives (silica fume, fly ash, and blast furnace slag), in binary and ternary systems were used. The experimental study included uniaxial tensile coupon testing on composite specimens and bond tests on composite material applied to clay-brick substrate. The results obtained with the different textile-reinforced cement-based mortars were compared and are discussed here. It was found that, for mortar formulations containing mineral additives—such as fly ash or blast-furnace slag—with high tensile and bond strengths, an adequate adherence between the constituents was obtained. The developed mortar presents mechanical performances equivalent to traditional mortars without additives. The study contributes to the existing knowledge regarding the structural behavior of TRM and promotes the development of a low impact carbon cementitious matrix.
Mechanical Properties of Thin-Ply Composites Based on Acoustic Emission Technology
Authors: Zheng Kaidong , Cao Dongfeng , Hu Haixiao , Ji Yundong , Li Shuxin
Published: February 2021

Compared with standard-ply composites, thin-ply composites exhibit a superior mechanical performance under various operating conditions due to their positive size effects. Thin-ply laminate failure modes, including matrix initial damage (MID), matrix failure (MF), and fiber failure (FF), have been distinguished through a systematic acoustic emission (AE) signals analysis combined with scanning electron microscopy (SEM). First, the characteristic frequencies of various failure modes are identified based on unidirectional laminates ([90] 68 and [0] 68). Then, according to the identified frequencies corresponding to distinctive damage modes, four lay-up sequences (02[[90m/0m]ns]02, m = 1, 2, 4, 8, n × m = 16) with a constant total thickness are designed, and the effects of the number of identical plies in the laminate thickness on the damage evolution characteristics and the damage process under uniaxial tension loads are dynamically monitored. The obtained results indicate that the characteristic frequency ranges for MID, MF, and FF are identified as 0–85 kHz, 165–260 kHz, and 261–304 kHz, respectively. The thickness of identical plies has a significant effect on onset damage. With the decrease of the number of identical plies (i.e., m in the stacking sequences), the thin-ply laminates exhibit the initiation of damage suppression effects and crack propagation resistance.
Physicochemical Properties of Biobutanol as an Advanced Biofuel
Authors: Obergruber Michal , Hönig Vladimír , Procházka Petr , Kučerová Viera , Kotek Martin , Bouček Jiří , Mařík Jakub
Published: February 2021

Biobutanol is a renewable, less polluting, and potentially viable alternative fuel to conventional gasoline. Biobutanol can be produced from same sources as bioethanol, and it has many advantages over the widespread bioethanol. This paper systematically analyzes biobutanol fuel as an alternative to bioethanol in alcohol–gasoline mixtures and the physicochemical properties. Based on the conducted analyses, it was found that biobutanol mixtures have a more suitable behavior of vapor pressure without the occurrence of azeotrope, do not form a separate phase in lower temperature, it has higher energy density, but slightly reduce the octane number and a have higher viscosity. However, in general, biobutanol has many advantageous properties that could allow its use in gasoline engines instead of the commonly used bioethanol.
Hydrothermal Synthesis of TiO2 Aggregates and Their Application as Negative Electrodes for Lithium-Ion Batteries: The Conflicting Effects of Specific Surface and Pore Size
Authors: Mehraz Saida , Luo Wenpo , Swiatowska Jolanta , Bezzazi Boudjema , Taleb Abdelhafed
Published: February 2021

TiO2 aggregates of controlled size have been successfully prepared by hydrothermal synthesis using TiO2 nanoparticles of different sizes as a building unit. In this work, different techniques were used to characterize the as-prepared TiO2 aggregates, e.g., X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), Brunauer, Emmett and Teller technique (BET), field emission gun scanning electron microscopy (FEGSEM), electrochemical measurements etc. The size of prepared TiO2 aggregates varied from 10–100 nm, and their pore size from around 5–12 nm; this size has been shown to depend on synthesis temperature. The mechanism of the aggregate formations was discussed in terms of efficiency of collision and coalescence processes. These newly synthetized TiO2 aggregates have been investigated as potential negative insertion electrode materials for lithium-ion batteries. The influence of specific surface areas and pore sizes on the improved capacity was discussed—and conflicting effects pointed out.
CFRP Origami Metamaterial with Tunable Buckling Loads: A Numerical Study
Authors: Zhu Houyao , Chen Shouyan , Shen Teng , Wang Ruikun , Liu Jie
Published: February 2021

Origami has played an increasingly central role in designing a broad range of novel structures due to its simple concept and its lightweight and extraordinary mechanical properties. Nonetheless, most of the research focuses on mechanical responses by using homogeneous materials and limited studies involving buckling loads. In this study, we have designed a carbon fiber reinforced plastic (CFRP) origami metamaterial based on the classical Miura sheet and composite material. The finite element (FE) modelling process’s accuracy is first proved by utilizing a CFRP plate that has an analytical solution of the buckling load. Based on the validated FE modelling process, we then thoroughly study the buckling resistance ability of the proposed CFRP origami metamaterial numerically by varying the folding angle, layer order, and material properties, finding that the buckling loads can be tuned to as large as approximately 2.5 times for mode 5 by altering the folding angle from 10° to 130°. With the identical rate of increase, the shear modulus has a more significant influence on the buckling load than Young’s modulus. Outcomes reported reveal that tunable buckling loads can be achieved in two ways, i.e., origami technique and the CFRP material with fruitful design freedoms. This study provides an easy way of merely adjusting and controlling the buckling load of lightweight structures for practical engineering.
Model-Based Design Approach to Improve Performance Characteristics of Hydrostatic Bearing Using Multivariable Optimization
Authors: Rehman Waheed Ur , Wang Xinhua , Cheng Yiqi , Chen Yingchun , Shahzad Hasan , Chai Hui , Abbas Kamil , Ullah Zia , Kanwal Marya
Published: February 2021

Research in the field of tribo-mechatronics has been gaining popularity in recent decades. The objective of the current research is to improve static/dynamics characteristics of hydrostatic bearings. Hydrostatic bearings always work in harsh environmental conditions that effect their performance, and which may even result in their failure. The current research proposes a mathematical model-based system for hydrostatic bearings that helps to improve its static/dynamic characteristics under varying conditions of performance-influencing variables such as temperature, spindle speed, external load, and clearance gap. To achieve these objectives, the capillary restrictors are replaced with servo valves, and a mathematical model is developed along with robust control design systems. The control system consists of feedforward and feedback control techniques that have not been applied before for hydrostatic bearings in the published literature. The feedforward control tries to remove a disturbance before it enters the system while feedback control achieves the objective of disturbance rejection and improves steady-state characteristics. The feedforward control is a trajectory-based controller and the feedback controller is a sliding mode controller with a PID sliding surface. The particle swarm optimization algorithm is used to tune the 6-dimensional vector of the tuning parameters with multi-objective performance criteria. Numerical investigations have been carried out to check the performance of the proposed system under varying conditions of viscosity, clearance gap, external load and the spindle speed. The comparison of our results with the published literature shows the effectiveness of the proposed system.
Paracellular Filtration Secretion Driven by Mechanical Force Contributes to Small Intestinal Fluid Dynamics
Authors: Buddington Randal K. , Wong Thomas , Howard Scott C.
Published: February 2021

Studies of fluid secretion by the small intestine are dominated by the coupling with ATP-dependent generation of ion gradients, whereas the contribution of filtration secretion has been overlooked, possibly by the lack of a known mechanistic basis. We measured apical fluid flow and generation of hydrostatic pressure gradients by epithelia of cultured mouse enterocytes, Caco-2 and T-84 cells, and fibroblasts exposed to mechanical force provided by vigorous aeration and in response to ion gradients, inhibitors of ion channels and transporters and in vitro using intact mouse and rat small intestine. We describe herein a paracellular pathway for unidirectional filtration secretion that is driven by mechanical force, requires tight junctions, is independent of ionic and osmotic gradients, generates persistent hydrostatic pressure gradients, and would contribute to the fluid shifts that occur during digestion and diarrhea. Zinc inhibits the flow of fluid and the paracellular marker fluorescein isothyocyanate conjugated dextran (MW = 4 kD) across epithelia of cultured enterocytes (>95%; p < 0.001) and intact small intestine (>40%; p = 0.03). We propose that mechanical force drives fluid secretion through the tight junction complex via a “one-way check valve” that can be regulated. This pathway of filtration secretion complements chloride-coupled fluid secretion during high-volume fluid flow. The role of filtration secretion in the genesis of diarrhea in intact animals needs further study. Our findings may explain a potential linkage between intestinal motility and intestinal fluid dynamics.
Emerging Utility of Applied Magnetic Resonance Imaging in the Management of Traumatic Brain Injury
Authors: Nadel Jeffrey , McNally Joseph Scott , DiGiorgio Anthony , Grandhi Ramesh
Published: February 2021

Traumatic brain injury (TBI) is a widespread and expensive problem globally. The standard diagnostic workup for new TBI includes obtaining a noncontrast computed tomography image of the head, which provides quick information on operative pathologies. However, given the limited sensitivity of computed tomography for identifying subtle but meaningful changes in the brain, magnetic resonance imaging (MRI) has shown better utility for ongoing management and prognostication after TBI. In recent years, advanced applications of MRI have been further studied and are being implemented as clinical tools to help guide care. These include functional MRI, diffusion tensor imaging, MR perfusion, and MR spectroscopy. In this review, we discuss the scientific basis of each of the above techniques, the literature supporting their use in TBI, and how they may be clinically implemented to improve the care of TBI patients.
Exploitation and Wear Properties of Nanostructured WC-Co Tool Modified with Plasma-Assisted Chemical Vapor Deposition TiBN Coating
Authors: Musa Mateja Šnajdar , Sakoman Matija , Ćorić Danko , Aleksandrov Fabijanić Tamara
Published: February 2021

In recent years, coated cemented carbides have often been the first choice for a wide variety of tool inserts and applications. Its success as a cutting tool material arises from the unique combination of wear resistance and toughness, and its ability to be formed into complex shapes. The structure obtained by sintering nanoparticle powders provides a significant improvement in product properties, such as higher cutting speeds, lower tool tolerances, and longer service life. In this study, a multi-layered gradient coating, deposited on nanostructured cemented carbides by plasma-assisted chemical vapor deposition (PACVD) was investigated with emphasis on its wear and exploitation properties. TiBN coating was deposited on nanostructured cemented carbide samples with the addition of 5 wt% Co, 10 wt% Co and 15 wt% Co. The samples were consolidated by one cycle hot isostatic pressing (HIP) technique. Complex architecture built of TiN and TiB2 gradient multilayer sequence block was deposited on each type of substrate. Wear resistance of the obtained samples was determined by erosion wear testing and dry sliding wear testing (ball-on-flat test). The friction coefficients of ~0.22 obtained for coated samples by the ball-on-flat test show a decrease in friction when compared to uncoated samples values of ~0.32. The absence of coating rupture was confirmed by wear track depth measurements showing a wear trace depth of ~1.2 μm. Exploitation properties i.e., tool life determination of samples was obtained using single-point turning tool test and compared to commercial cutting tool insert type K10 tested under the same conditions. All the conducted tests show excellent wear and exploitation properties of the newly developed TiBN coating under chosen conditions, including cutting speed, vc = 200 m/min, depth of cut, ap = 1 mm, and feed, fn = 0.2 mm. Coated WC-Co samples with 15 wt% Co, having withstood 15 min of machining with flank wear trace size less than 0.3 mm, suggest significant improvement when compared to trace size of 0.56 mm obtained for K10 commercial cutting insert.
Surface-Potential-Based Compact Modeling of p-GaN Gate HEMTs
Authors: Wang Jie , Chen Zhanfei , You Shuzhen , Bakeroot Benoit , Liu Jun , Decoutere Stefaan
Published: February 2021

We propose a surface potential (SP)-based compact model of p-GaN gate high electron mobility transistors (HEMTs) which solves the Poisson equation. The model includes all possible charges in the GaN channel layer, including the unintended Mg doping density caused by out-diffusion. The SP equation and its analytical approximate solution provide a high degree of accuracy for the SP calculation, from which the closed-form I–V equations are derived. The proposed model uses physical parameters only and is implemented in Verilog-A code.
Research on Precision Blanking Process Design of Micro Gear Based on Piezoelectric Actuator
Authors: Hu Changjun , Shi Yunyang , Liu Fangfang
Published: February 2021

In order to process micro scale parts more conveniently, especially the micro parts with complex shape, a new micro blanking equipment based on piezoelectric ceramic driving is proposed in this paper. Compared with other large precision machining equipment, the equipment cost has been greatly reduced. Using displacement sensor to detect the change of output displacement and feedback control piezoelectric actuator to control the change of relevant parameters, the control precision is high. The micro gear parts with diameter less than 2 mm are obtained through the blanking experiment on the experimental equipment. From the relationship between the obtained time and the punch output force, output displacement and die adjustment, it can be seen that the designed equipment has good processing performance and can complete the blanking forming of micro parts well.
Cryopreservation of Cyanobacteria and Eukaryotic Microalgae Using Exopolysaccharide Extracted from a Glacier Bacterium
Authors: Ali Pervaiz , Fucich Daniel , Shah Aamer Ali , Hasan Fariha , Chen Feng
Published: February 2021

Exopolysaccharide (EPS) has been known to be a good cryoprotective agent for bacteria, but it has not been tested for cyanobacteria and eukaryotic microalgae. In this study, we used EPS extracted from a glacier bacterium as a cryoprotective agent for the cryopreservation of three unicellular cyanobacteria and two eukaryotic microalgae. Different concentrations of EPS (10%, 15%, and 20%) were tested, and the highest concentration (20%) of EPS yielded the best growth recovery for the algal strains we tested. We also compared EPS with 5% dimethyl sulfoxide (DMSO) and 10% glycerol for the cryopreservation recovery. The growth recovery for the microalgal strains after nine months of cryopreservation was better than 5% DMSO, a well-known cryoprotectant for microalgae. A poor recovery was recorded for all the tested strains with 10% glycerol as a cryoprotective agent. The patterns of growth recovery for most of these strains were similar after 5 days, 15 days, and 9 months of cryopreservation. Unlike common cryopreservants such as DMSO or methanol, which are hazardous materials, EPS is safe to handle. We demonstrate that the EPS from a psychrotrophic bacterium helped in the long-term cryopreservation of cyanobacteria and microalgae, and it has the potential to be used as natural cryoprotective agent for other cells.
Comparison of Three Serological Methods for the Epidemiological Investigation of TBE in Dogs
Authors: Girl Philipp , Haut Maja , Riederer Sandra , Pfeffer Martin , Dobler Gerhard
Published: February 2021

Tick-borne encephalitis (TBE) virus is an emerging pathogen that causes severe infections in humans. Infection risk areas are mostly defined based on the incidence of human cases, a method which does not work well in areas with sporadic TBE cases. Thus, sentinel animals may help to better estimate the existing risk. Serological tests should be thoroughly evaluated for this purpose. Here, we tested three test formats to assess the use of dogs as sentinel animals. A total of 208 dog sera from a known endemic area in Southern Germany were tested in an All-Species-ELISA and indirect immunofluorescence assays (IIFA), according to the manufacturer’s instructions. Sensitivity and specificity for both were determined in comparison to the micro-neutralization test (NT) results. Of all 208 samples, 22.1% tested positive in the micro-NT. A total of 18.3% of the samples showed characteristic fluorescence in the IIFA and were, thus, judged positive. In comparison to the micro-NT, a sensitivity of 78.3% and a specificity of 98.8% was obtained. In the ELISA, 19.2% of samples tested positive, with a sensitivity of 84.8% and a specificity of 99.4%. The ELISA is a highly specific test for TBE-antibody detection in dogs and should be well suited for acute diagnostics. However, due to deficits in sensitivity, it cannot replace the NT, at least for epidemiological studies. With even lower specificity and sensitivity, the same applies to IIFA.
The Solution Interaction of Tetrathionate Ions and Sodium Isobutyl Xanthate and Its Effect on the Flotation of Galena and Chalcopyrite
Authors: Mhonde Ngoni , Pitkänen Leena , Corin Kirsten , Schreithofer Nóra
Published: February 2021

Tetrathionates have been found in significantly high concentrations in recycled process waters from massive sulphide ore processing plants. These polythionates react with xanthate added to flotation pulps thus reducing xanthate dosages in solution potentially affecting flotation performance. The current study focused on the effect of the tetrathionate-xanthate reaction on sulphide mineral recoveries. Ore dissolution studies confirmed the generation of tetrathionates by copper-lead-zinc ores. In 20 min, the tetrathionates consumed more than half of the xanthate in solution at pH 7. Rest potential measurements and Fourier transform infrared spectroscopy (FTIR) showed that the degree of collector-mineral interactions of xanthate and both galena and chalcopyrite was greatly reduced in the presence of a 2000 mg/L tetrathionate solution. Microflotation tests showed that chalcopyrite recovery was less sensitive to tetrathionates as indicated by small changes in mineral recoveries. Galena was sensitive to the action of tetrathionates on the mineral surface as the galena recovery significantly declined when floated with xanthate as a collector in both a 500 mg/L tetrathionate solution and a 2000 mg/L tetrathionate solution. These fundamental results lay a sound base on which more discussion into the significance and the effect of tetrathionates on flotation performance of sulphide ores can be developed.
Characterization of Fines Produced by Degradation of Polymetallic Nodules from the Clarion–Clipperton Zone
Authors: Kim Mun Gi , Hyeong Kiseong , Yoo Chan Min , Lee Ji Yeong , Seo Inah
Published: February 2021

The discharge of fluid–particle mixture tailings can cause serious disturbance to the marine environment in deep-sea mining of polymetallic nodules. Unrecovered nodule fines are one of the key components of the tailings, but little information has been gained on their properties. Here, we report major, trace, and rare earth element compositions of <63 μm particles produced by the experimental degradation of two types of polymetallic nodules from the Clarion–Clipperton Zone. Compared to the bulk nodules, the fines produced are enriched in Al, K, and Fe and depleted in Mn, Co, Ni, As, Mo, and Cd. The deviation from the bulk composition of original nodules is particularly pronounced in the finer fraction of particles. With X-ray diffraction patterns showing a general increase in silicate and aluminosilicates in the fines, the observed trends indicate a significant contribution of sediment particles released from the pores and cracks of nodules. Not only the amount but also the composition of nodule fines is expected to significantly differ depending on the minimum recovery size of particles at the mining vessel.
A Comparative Study on the Efficiency of Reliability Methods for the Probabilistic Analysis of Local Scour at a Bridge Pier in Clay-Sand-Mixed Sediments
Authors: Jafari-Asl Jafar , Ben Seghier Mohamed El Amine , Ohadi Sima , Dong You , Plevris Vagelis
Published: February 2021

In this work, the performance of reliability methods for the probabilistic analysis of local scour at a bridge pier is investigated. The reliability of bridge pier scour is one of the important issues for the risk assessment and safety evaluation of bridges. Typically, the depth prediction of bridge pier scour is estimated using deterministic equations, which do not consider the uncertainties related to scour parameters. To consider these uncertainties, a reliability analysis of bridge pier scour is required. In the recent years, a number of efficient reliability methods have been proposed for the reliability-based assessment of engineering problems based on simulation, such as Monte Carlo simulation (MCS), subset simulation (SS), importance sampling (IS), directional simulation (DS), and line sampling (LS). However, no general guideline recommending the most appropriate reliability method for the safety assessment of bridge pier scour has yet been proposed. For this purpose, we carried out a comparative study of the five efficient reliability methods so as to originate general guidelines for the probabilistic assessment of bridge pier scour. In addition, a sensitivity analysis was also carried out to find the effect of individual random variables on the reliability of bridge pier scour.
Synthesis and Structural Characterization of a Silver(I) Pyrazolato Coordination Polymer
Authors: Fujisawa Kiyoshi , Nemoto Takuya , Morishima Yui , Leznoff Daniel B.
Published: February 2021

Coinage metal(I)···metal(I) interactions are widely of interest in fields such as supramolecular assembly and unique luminescent properties, etc. Only two types of polynuclear silver(I) pyrazolato complexes have been reported, however, and no detailed spectroscopic characterizations have been reported. An unexpected synthetic method yielded a polynuclear silver(I) complex [Ag(μ-L1Clpz)]n (L1Clpz− = 4-chloride-3,5-diisopropyl-1-pyrazolate anion) by the reaction of {[Ag(μ-L1Clpz)]3}2 with (nBu4N)[Ag(CN)2]. The obtained structure was compared with the known hexanuclear silver(I) complex {[Ag(μ-L1Clpz)]3}2. The Ag···Ag distances in [Ag(μ-L1Clpz)]n are slightly shorter than twice Bondi’s van der Waals radius, indicating some Ag···Ag argentophilic interactions. Two Ag–N distances in [Ag(μ-L1Clpz)]n were found: 2.0760(13) and 2.0716(13) Å, and their N–Ag–N bond angles of 180.00(7)° and 179.83(5)° indicate that each silver(I) ion is coordinated by two pyrazolyl nitrogen atoms with an almost linear coordination. Every five pyrazoles point in the same direction to form a 1-D zig-zag structure. Some spectroscopic properties of [Ag(μ-L1Clpz)]n in the solid-state are different from those of {[Ag(μ-L1Clpz)]3}2 (especially in the absorption and emission spectra), presumably attributable to this zig-zag structure having longer but differently arranged intramolecular Ag···Ag interactions of 3.39171(17) Å. This result clearly demonstrates the different physicochemical properties in the solid-state between 1-D coordination polymer and metalacyclic trinuclear (hexanuclear) or tetranuclear silver(I) pyrazolate complexes.
Public Interest in Neurological Diseases on Wikipedia during Coronavirus Disease (COVID-19) Pandemic
Authors: Rutovic Stela , Fumagalli Ana Isabel , Lutsenko Inna , Corea Francesco
Published: February 2021

Infodemiology is a research discipline that investigates parameters of information distribution in order to support public health and public policy. Wikipedia, a free online encyclopedia, is commonly used as a source of information for infodemiological studies. Using Pageviews analysis, we descriptively assessed the total monthly number of views of the Wikipedia articles in English describing main neurological diseases in the period from January 2018 to July 2020. Our results show a general trend of a decrease in interest in neurological disease-related pages throughout years and especially during the burst of interest towards coronavirus. The monitoring of infodemiological indicators shall be prioritized to reshape global campaigns and tailored advocacy programs.
Omega-3 Polyunsaturated Fatty Acids Prevent Nonalcoholic Steatohepatitis (NASH) and Stimulate Adipogenesis
Authors: Antraco Vitor Jacó , Hirata Bruna Kelly Sousa , de Jesus Simão Jussara , Cruz Maysa Mariana , da Silva Viviane Simões , da Cunha de Sá Roberta Dourado Cavalcante , Abdala Fernanda Miranda , Armelin-Correa Lucia , Alonso-Vale Maria Isabel Cardoso
Published: February 2021

The increasing impact of obesity on global human health intensifies the importance of studies focusing on agents interfering with the metabolism and remodeling not only of the white adipose tissue (WAT) but also of the liver. In the present study, we have addressed the impact of n-3 PUFA in adipose cells’ proliferation and adipogenesis, as well as in the hepatic lipid profile and morphology. Mice were induced to obesity by the consumption of a high-fat diet (HFD) for 16 weeks. At the 9th week, the treatment with fish oil (FO) was initiated and maintained until the end of the period. The FO treatment reduced the animals’ body mass, plasma lipids, glucose, plasma transaminases, liver mass, triacylglycerol, and cholesterol liver content when compared to animals consuming only HFD. FO also decreased the inguinal (ing) WAT mass, reduced adipocyte volume, increased adipose cellularity (hyperplasia), and increased the proliferation of adipose-derived stromal cells (AdSCs) which corroborates the increment in the proliferation of 3T3-L1 pre-adipocytes or AdSCs treated in vitro with n-3 PUFA. After submitting the in vitro treated (n-3 PUFA) cells, 3T3-L1 and AdSCs, to an adipogenic cocktail, there was an increase in the mRNA expression of adipogenic transcriptional factors and other late adipocyte markers, as well as an increase in lipid accumulation when compared to not treated cells. Finally, the expression of browning-related genes was also higher in the n-3 PUFA treated group. We conclude that n-3 PUFA exerts an attenuating effect on body mass, dyslipidemia, and hepatic steatosis induced by HFD. FO treatment led to decreasing adiposity and adipocyte hypertrophy in ingWAT while increasing hyperplasia. Data suggest that FO treatment might induce recruitment (by increased proliferation and differentiation) of new adipocytes (white and/or beige) to the ingWAT, which is fundamental for the healthy expansion of WAT.
Circulating Levels of Sclerostin Predict Glycemic Improvement after Sleeve Gastrectomy
Authors: Carbone Federico , Nulli Migliola Elisa , Bonaventura Aldo , Vecchié Alessandra , De Vuono Stefano , Ricci Maria Anastasia , Vaudo Gaetano , Boni Marcello , Ministrini Stefano , Lupattelli Graziana , Montecucco Fabrizio
Published: February 2021

Among the different effects of bariatric surgery, here we focus on bone-derived inflammatory molecules, and in particular, sclerostin; an osteocyte product potentially associated with cardio-metabolic diseases. In 94 morbidly obese patients undergoing laparoscopic sleeve gastrectomy (SG), over-time changes in anthropometric and biochemical measures—including insulin resistance (IR) indexes—were correlated with serum sclerostin levels. Sclerostin was positively associated with anthropometric indexes of obesity, and inversely with IR, namely homeostatic model assessment for peripheral insulin sensitivity (HOMA2%S) (r = −0.218; p = 0.045). Sclerostin emerged as the only significant predictor of HOMA2-%S normalization, independently of demographic and anthropometric variables (OR 1.01 (95% CI 1.00–1.02); p = 0.024). We also identified two distinct patterns of serum sclerostin change: the higher/lower sclerostin levels at baseline, the greater their post-surgical reduction/increase (p < 0.001 for all subgroups). Among those two patterns, especially the post-surgery increase in serum sclerostin was associated with lean mass reduction, without any association with IR indexes. Although counterintuitive, this change was likely dependent on the post-surgical increase in bone turnover. In conclusion, baseline serum levels of sclerostin correlate with anthropometric measures of obesity and IR, and the ability to predict glycemic improvements after SG. Specifically, serum sclerostin was closely associated with peripheral insulin sensitivity (HOMA2-%S), thus supporting the role of skeletal muscle/bone interactions in metabolic diseases.
Different Dietary N-3 Polyunsaturated Fatty Acid Formulations Distinctively Modify Tissue Fatty Acid and N-Acylethanolamine Profiles
Authors: Murru Elisabetta , Lopes Paula A. , Carta Gianfranca , Manca Claudia , Abolghasemi Armita , Guil-Guerrero José L. , Prates José A. M. , Banni Sebastiano
Published: February 2021

We investigated the influence of different dietary formulation of n-3 polyunsaturated fatty acids (PUFA) on rat tissue fatty acid (FA) incorporation and consequent modulation of their bioactive metabolite N-acylethanolamines (NAE). For 10 weeks, rats were fed diets with 12% of fat from milk + 4% soybean oil and 4% of oils with different n-3 PUFA species: soybean oil as control, linseed oil rich in α-linolenic (ALA), Buglossoides arvensis oil rich in ALA and stearidonic acid (SDA), fish oil rich in eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), Nannochloropsis microalga oil rich in EPA or Schizochytrium microalga oil rich in DHA. FA and NAE profiles were determined in plasma, liver, brain and adipose tissues. Different dietary n-3 PUFA distinctively influenced tissue FA profiles and consequently NAE tissue concentrations. Interestingly, in visceral adipose tissue the levels of N-arachidonoylethanolamide (AEA) and N-docosahexaenoylethanolamide (DHEA), NAE derived from arachidonic acid (AA) and DHA, respectively, significantly correlated with NAE in plasma, and circulating DHEA levels were also correlated with those in liver and brain. Circulating NAE derived from stearic acid, stearoylethanolamide (SEA), palmitic acid and palmitoylethanolamide (PEA) correlated with their liver concentrations. Our data indicate that dietary n-3 PUFA are not all the same in terms of altering tissue FA and NAE concentrations. In addition, correlation analyses suggest that NAE levels in plasma may reflect their concentration in specific tissues. Given the receptor-mediated tissue specific metabolic role of each NAE, a personalized formulation of dietary n-3 PUFA might potentially produce tailored metabolic effects in different pathophysiological conditions.
Postoperative Ileus after Stimulation with Probiotics before Ileostomy Closure
Authors: Rodríguez-Padilla Ángela , Morales-Martín Germán , Pérez-Quintero Rocío , Gómez-Salgado Juan , Balongo-García Rafael , Ruiz-Frutos Carlos
Published: February 2021

Loop ileostomy closure after colorectal surgery is often associated with Postoperative ileus, with an incidence between 13–20%. The aim of this study is to evaluate the efficacy and safety of preoperative stimulation of the efferent loop with probiotics prior to ileostomy closure in patients operated on for colorectal carcinoma. For this, a prospective, randomized, double-blind, controlled study is designed. All patients who underwent surgery for colorectal carcinoma with loop ileostomy were included. Randomized and divided into two groups, 34 cases and 35 controls were included in the study. Postoperative ileus, the need for nasogastric tube insertion, the time required to begin tolerating a diet, restoration of bowel function, and duration of hospital stay were evaluated. The incidence of Postoperative ileus was similar in both groups, 9/34 patients stimulated with probiotics and 10/35 in the control group (CG) with a p = 0.192. The comparative analysis showed a direct relationship between Postoperative ileus after oncological surgery and Postoperative ileus after reconstruction surgery, independently of stimulation. Postoperative ileus after closure ileostomy is independent of stimulation of the ileostomy with probiotics through the efferent loop. There seem to be a relationship between Postoperative ileus after reconstruction and the previous existence of Postoperative ileus after colorectal cancer surgery.
The Christian Orthodox Church Fasting Diet Is Associated with Lower Levels of Depression and Anxiety and a Better Cognitive Performance in Middle Life
Authors: Spanaki Cleanthe , Rodopaios Nikolaos E. , Koulouri Alexandra , Pliakas Triantafyllos , Papadopoulou Sousana K. , Vasara Eleni , Skepastianos Petros , Serafeim Tatiana , Boura Iro , Dermitzakis Emmanouil , Kafatos Anthony
Published: February 2021

Lifestyle choices significantly influence mental health in later life. In this study we investigated the effects of the Christian Orthodox Church (COC) fasting diet, which includes long-term regular abstinence from animal-based products for half the calendar year, on cognitive function and emotional wellbeing of healthy adults. Two groups of fasting and non-fasting individuals were evaluated regarding their cognitive performance and the presence of anxiety and depression using the Mini Mental Examination Scale, the Hamilton Anxiety Scale, and the Geriatric Depression Scale (GDS), respectively. Data on physical activity, smoking, and vitamin levels were collected and correlated with mental health scoring. Negative binomial regression was performed to examine differences in the GDS scores between the two groups. Significantly lower levels of anxiety (7.48 ± 4.98 vs. 9.71 ± 5.25; p < 0.001) and depression (2.24 ± 1.77 vs. 3.5 ± 2.52; p < 0.001), along with better cognitive function (29.15 ± 0.79 vs. 28.64 ± 1.27; p < 0.001), were noticed in fasting compared to non-fasting individuals. GDS score was 31% lower (Incidence Rate Ratio: 0.69, 95% Confidence Interval: 0.56–0.85) in the fasting group compared to the control, while vitamin and ferrum levels did not differ. The COC fasting diet was found to have an independent positive impact on cognition and mood in middle-aged and elderly individuals.