bluuebunny/crossref_metadata_2025 huggingface dataset:
Abstract WP216: Epigenome-Wide Association Study of Cerebral White Matter Hyperintensities
Authors: Fornage Myriam , Adams Hieab H , Bis Joshua C , Deary Ian J , Launer Lenore , Smith Jennifer A , Teumer Alexander , Seshadri Sudha ,   NeuroCHARGE
Published: January 2019

Background:
Cerebral white matter hyperintensities (WMH) on MRI are typical markers of small vessel disease and strong radiological correlates of stroke and age-related cognitive decline. Despite a recognized high heritability, the molecular basis of WMH has not been fully characterized. In particular, the contribution of epigenetic modifications, such as DNA methylation, has not been explored.

Methods:
We conducted a meta-analysis of epigenome-wide association studies of WMH burden in up to 5777 elderly participants of European and African ancestry from 9 population-based cohorts using blood-derived DNA methylation measured on the Infinium HumanMethylation450 BeadChip. Cohort-specific associations between burden of WMH and DNA methylation beta values were estimated using linear mixed-effect models and combined in a sample-size weighted fixed-effect meta-analysis. In addition, we used two different approaches to identify differentially methylated regions (DMRs), which may be more informative than individual loci. Bonferroni correction and False Discovery Rates were used to account for the multiple tests.

Results:
Single-site analyses identified a CpG site within the CLDN5 gene significantly associated with WMH (P= 2.3x10
-7
). Claudin-5 is an endothelial-specific component in the brain vasculature and regulates blood brain barrier permeability. Region-based analyses, which leverage the correlations between nearby CpG sites identified several DMRs significantly associated with WMH burden including: PRMT1, BTBD17, and IFITM10 (P=1.4x10
-10
, 2.3x10
-8
, and 3.6x10
-7
, respectively). PRMT1 encodes the Protein Arginine N-methylase 1, which methylates histones in genes involved in glioblastomagenesis. Mice lacking this gene are characterized by severe defects in oligodendrocyte maturation processes. The function of BTBD17 and IFITM10 is not characterized but both are expressed in the brain and exhibit changes in expression in response to viral infection.

Conclusions:
Consistent with our previously reported genetic association studies, this genome-wide DNA methylation analysis supports a role of genes involved in glial cell function in WMH etiology. It also suggests a novel role of genes involved in viral response.
Abstract WP330: Refining Prehospital Stroke Severity Measures: Is There Added Benefit to Combining Field Glasgow Coma Scale and Los Angeles Motor Score?
Authors: Cheung Shauna , Shkirkova Kristina , Liebeskind David , Sabra Mark , Starkman Sidney , Hamilton Scott , Saver Jeffery L ,   FAST-MAG Investigators, Sanossian Nerses
Published: January 2019

Background:
Prehospital research requires severity screening instruments that can be reliably performed in the field and that are validated for predicting 3-month outcome. The Los Angeles Motor Scale and Glasgow coma scale are regularly performed in the field. The LAMS, a pure motor scale typically used to screen for LVO, has been validated as a stroke severity measure which correlates well with 90-day outcome.

Objective:
to determine if the combination of GCS+LAMS better predicts stroke severity at presentation and long-term outcomes than either instrument alone.

Methods:
All subjects were participating in the FAST-MAG clinical trial. LAMS and GCS measures were obtained by paramedics in the field. NIHSS was obtained by study nurses after hospital arrival, mRS was obtained at 90 days. The composite LAMS+GCS was calculated by adding one point for every point lost on the GCS (1-12 possible) to the LAMS score (0-10 possible). Receiver operating characteristics were calculated for 90-day outcome LAMS, GCS and LAMS+GCS with determination of best fit. Correlation was calculated for ED NIHSS.

Results:
Among 1700 patients (age 69±13 years, female 42.6%) 73.3% of patients had final diagnosis of cerebral ischemia, 22.8% intracranial hemorrhage, and 3.9% stroke mimic. Time from onset to prehospital LAMS was median 30 minutes (IQR 20-50). Predictive accuracy (C statistics) for poor 3-month outcome (mRS 3-6 at day 90) in all patients was 0.69 for LAMS+GCS, 0.68 for LAMS and 0.59 for GCS. Best fit for determining poor outcome was LAMS+GCS >=5 (Sensitivity 64%, Specificity 67%), LAMS >= 4 (Sensitivity 73%, specificity 56%), and GCS<=14 (92%, 45%). Correlation to hospital arrival NIHSS was strongest for the combined measure (R= 0.529) followed by LAMS (R=0.431) and GCS (R=-0.401). Among ICH cases (N=386) LAMS+GCS performed better than LAMS or GCS alone (C-statistic 0.693 vs. 0.642 vs. 0.600).

Conclusions:
Adding GCS to LAMS in the prehospital assessment does not improve its ability to identify patients who are likely to have poor outcomes. LAMS+GCS may be a better measure in ICH cases or in predicting initial prehospital stroke severity, capturing non-motor deficits. LAMS alone can be utilized as a stroke severity instrument in prehospital clinical trials.
Abstract WP448: Safety of Heparin for Prophylaxis of Venous Thromboembolism in Oral Anticoagulation-Associated Intracerebral Hemorrhage
Authors: Sembill Jochen A , Sprügel Maximilian I , Kuramatsu Joji B , Gerner Stefan T , Dörfler Arnd , Schwab Stefan , Huttner Hagen ,   RETRACE I+II collaborators
Published: January 2019

Introduction:
Patients with intracerebral hemorrhage (ICH) have a high risk of venous thromboembolism (VTE). Next to intermittent pneumatic compressions low-dose subcutaneous heparins represent the most intuitive treatment for VTE prophylaxis. However, in the specific setting of ICH their safety remains to be verified as randomized controlled trials are missing. The present study pooled individual data of patients with spontaneous primary ICH and OAC-ICH to explore the incidence of hemorrhagic complications during hospital stay among subgroups treated with heparins for VTE prophylaxis.

Methods:
We integrated both parts of the RETRACE-program (part-1: 2006-2010; part-2:2011-2015) and the single-center UKER-ICH registry (2006-2015). Including all patients receiving low-dose subcutaneous heparin for VTE prevention we pooled individual patient data of 1702 vitamin-K antagonist-(VKA) or non-VKA oral anticoagulants(NOAC)-related ICH patients treated at 22 tertiary-care centers across Germany and of 1022 primary spontaneous ICH patients from UKER. We defined intracranial hemorrhagic complications (IHC) during hospital stay as primary safety outcome measure. Secondary outcomes included mortality and functional outcome (modified Rankin Scale, mRS) at 3 months of patients with and without IHC.

Results:
IHC occurred in 1.7%(42/2416) of ICH patients. There were no differences in crude incidence rates among patients with VKA-ICH, NOAC-ICH and non-OAC-ICH (Log rank p=0.645; Breslow p=0.753; VKA-ICH: 27/1406[1.9%], NOAC-ICH 1/130[0.8%], non-OAC-ICH 14/880[1.6%];p=0.577). Detailed analysis according to days spent on heparin prophylaxis revealed no differences in rates of IHC per 1000 patient days (VKA-ICH: 1.49[1.00-2.14], NOAC-ICH 0.63[0.03-3.13], non-OAC-ICH 1.45[0.82-2.37]; p=0.687). Secondary outcomes showed differences in functional outcome (mRS=4-6: IHC: 29/37[78.4%] vs no-IHC: 1213/2048[59.2%];p=0.019) and mortality (IHC: 14/37[37.8%] vs no-IHC: 485/2048[23.7%];p=0.045) in disfavor of IHC-patients.

Conclusions:
Heparin administration for VTE prophylaxis in ICH patients appears to be safe without differently increased risks of IHC among non-OAC-ICH, VKA-ICH and NOAC-ICH.
Abstract WP462: New or Expanding Ventricular Hemorrhage Predicts Poor Outcome After Intracerebral Hemorrhage
Authors: Yogendrakumar Vignan , Ramsay Tim , Fergusson Dean , Demchuk Andrew M , Aviv Richard I , Rodriguez-Luna David , Molina Carlos A , Silva Blas Yolanda , Dzialowski Imanuel , Kobayashi Adam , Boulanger Jean-Martin , Lum Cheemun , Gubitz Gord , Padma Vasantha , Roy Jayanta , Kase Carlos S , Bhatia Rohit , Hill Michael D , Warren Andrew D , Anderson Christopher D , Gurol Mahmut E , Greenberg Steven M , Viswanathan Anand , Rosand Jonathan , Goldstein Joshua N , Dowlatshahi Dar ,   PREDICT/Sunnybrook CTA Study Group
Published: January 2019

Introduction:
Baseline intraventricular hemorrhage (IVH) is a predictor of poor outcome in acute intracerebral hemorrhage (ICH) patients. However, questions remain as to the exact burden that new IVH development, seen on follow-up imaging, or what degree of interval IVH expansion, impacts long term functioning.

Objective:
To derive and validate a relationship between IVH change and long term outcome.

Methods:
Fractional polynomial analysis was used to test linear and non-linear models of 24-hour IVH change and clinical outcome using data from the multicenter PREDICT study. The primary outcome was mRS 4-6 at 90 days. Dichotomous thresholds were derived via assessment of the selected model and diagnostic accuracy measures were calculated. Independent predictors of poor outcome were determined via multivariable logistic regression. The developed model and all derived thresholds were validated in an independent single center cohort.

Results:
Of the 256 patients from PREDICT, 127 (49.6%) had mRS scores of 4-6 at 90 days. 24-hour IVH change and the primary outcome fit a non-linear relationship, where minimal increases in IVH were associated with a high probability of poor outcome (Figure 1). Mean IVH expansion was 8.6 mL. IVH expansion greater than 1 mL (n=53, Sens 33%, Spec 92%, PPV 79%, NPV 58%, aOR 2.77 [95% CI: 1.12-6.89]) and development of any new IVH (n= 74, Sens 43%, Spec 85%, PPV 74%, NPV 60%, aOR 2.17 [95% CI: 1.02-4.63]) strongly predicted mRS 4-6 at 90 days. The model and developed thresholds reproduced well in a validation cohort of 170 patients.

Conclusion:
IVH expansion as minimal as 1 mL, or any new IVH is strongly predictive of poor outcome. This can aid in prognostication, be incorporated into definitions of hematoma expansion for future ICH treatment trials, or even imply that IVH treatment is a therapeutic target that may lead to improved outcomes.
Abstract WP538: Sex Differences in Intracranial and Extracranial Atherosclerosis in Patients With Acute Ischemic Stroke
Authors: Voigt Sabine , van Os Hendrikus J , van Walderveen Marianne A , van der Schaaf Irene C , Kappelle L. J , Broersen Alexander , Velthuis Birgitta K , de Jong Pim A , Kockelkoren Remko , Algra Ale , Wermer Marieke J ,   DUST study group
Published: January 2019

Objective:
To investigate sex differences in presence and location of atherosclerosis in a large population of acute ischaemic stroke patients.

Methods:
Participants were included from the Dutch acute stroke study (DUST), a large prospective multicentre cohort study. All patients received CT/CT-angiography within 9 hours of stroke onset. We assessed the presence of any sign of atherosclerosis in the intra- and extracranial circulation. In addition, we determined the burden of intracranial atherosclerosis by quantifying intracranial internal carotid artery (ICA) calcifications, resulting in calcium volumes. Relative risks between women and men were calculated using multivariable Poisson regression, and adjusted (aRR) for potential confounders (age, hypertension, hyperlipidemia, diabetes, smoking and alcohol use).

Results:
We included 1397 patients with a mean age of 67 years, of whom 600 (43%) were women. Presence of atherosclerosis in intracranial vessel segments was found as frequently in women as in men (71% versus 72%, aRR 0.95; 95%CI 0.89-1.01). In addition, intracranial ICA calcium volume did not differ between women and men (median volume 124 mm
3
, IQR 35.5-304.5 in women versus 112 mm
3
, IQR 33.0-276.5 in men; tertile largest volume 37% in women versus 33% in men, aRR 1.00; 95% CI 0.95-1.05). Extracranial atherosclerosis was less common in women compared with men (74% vs 81%, aRR 0.86; 95%CI 0.81-0.92). (Table)

Conclusions:
In patients with acute ischemic stroke the prevalence of intracranial atherosclerosis does not differ between women and men, while extracranial atherosclerosis is less often present in women compared with men.
Abstract WP54: MR CLEAN-MED - The Effect of Periprocedural Medication in Patients Undergoing Endovascular Treatment for Acute Ischemic Stroke: Heparin, Antiplatelet Agents, Both or Neither
Authors: van de Graaf Rob A , Roozenbeek Bob , Chalos Vicky , van Es Adriaan C , den Hertog Heleen M , Staals Julie , van Dijk Lukas , Jenniskens Sjoerd F , van der Lugt Aad , Dippel Diederik W ,   on behalf of the MR CLEAN MED investigators
Published: January 2019

Background:
Rapid endovascular treatment (EVT) in patients with acute ischemic stroke with confirmed proximal intracranial occlusion has been proven safe and effective. Still, many patients do not recover despite recanalization. Yet, it is unknown whether periprocedural antithrombotic medication in patients treated with EVT can improve clinical outcome. The objective of this study is to assess the effect of acetylsalicylic acid (ASA) and unfractionated heparin, alone, or in combination, in patients who undergo EVT.

Methods:
MR CLEAN-MED is a multicenter, prospective, randomized, open-label, blinded-endpoint trial using a 2x3 factorial design. We plan to enroll 1500 patients with a clinical diagnosis of acute ischemic stroke and confirmed intracranial anterior circulation occlusion, who will undergo EVT with or without prior intravenous thrombolysis according to standard care. Study interventions: IV treatment with ASA (300 mg), low dose unfractionated heparin (loading dose of 5000 IU followed by 500 IU/hour x 6 hours) and moderate dose unfractionated heparin (loading dose of 5000 IU followed by 1250 IU/hour x 6 hours). Primary outcome is the score on the modified Rankin Scale 90 days after inclusion in the study. Safety endpoints include the occurrence of symptomatic intracerebral hemorrhage.

Results:
First patient enrolled on January 22nd, 2018. 1 active enrolling site, 17 sites starting up.

Conclusion:
We hypothesize that despite the potentially increased risk of (symptomatic) intracerebral hemorrhage, periprocedural ASA and unfractionated heparin alone or in combination will improve functional outcome of patients with acute ischemic stroke treated with EVT.

Trial Registration Number:
ISRCTN 76741621

Funding:
Dutch Heart Foundation, Dutch Brain Foundation, Stryker
Abstract WP71: Multicenter ALADIN: Automated Large Artery Occlusion Detection in Stroke Imaging Using Artificial Intelligence
Authors: Rodrigues Gabriel M , Barreira Clara , Froehler Michael , Baxter Blaise , Devlin Thomas , Lim Jaims , Al-Bayati Alhamza R , Bouslama Mehdi , Haussen Diogo C , Nogueira Raul G
Published: January 2019

Introduction:
Large vessel occlusion (LVO) may account for up to 40% of all acute ischemic strokes (AIS) and may be associated with worse patient prognosis, being responsible for 60% of dependency and more than 90% of mortality after AIS. Therefore, accurate and rapid identification of LVO and notification of specialists is critical to maximizing the benefit of proven reperfusion therapies. Recent advances in artificial intelligence technology have facilitated the development of automated LVO detection on computed tomography angiography (CTA) imaging. This study evaluated the performance of the Viz LVO algorithm in AIS patients treated at four comprehensive stroke centers.

Methods:
We performed a multicenter retrospective analysis of 800 CTAs from 750 AIS patients admitted to three comprehensive stroke centers between 2014 and 2018. All studies were analyzed by the latest version of Viz LVO algorithm (version 4.1.2) and the performance was compared to expert neurointerventionalists’ reports. Algorithm run-time and notification time through the Viz platform times were also recorded for each CTA.

Results:
The Viz LVO algorithm demonstrated 92% sensitivity, 90% specificity in the analysis of 750 CTAs. The mean and maximum run time of the algorithm were 3 minutes and 6 minutes, respectively. Furthermore, mean time to notification was 6 minutes, with a maximum time to notification of 9 minutes.

Conclusions:
This multicenter retrospective study demonstrates fast and accurate performance of the Viz LVO algorithm in the detection and notification of LVOs on CTAs from three comprehensive stroke centers. Using artificial intelligence, this algorithm may permit early and accurate identification of LVO stroke patients and timely notification to emergency teams, enabling quick decision-making for reperfusion therapies or transfer to specialized centers if needed. Additional studies are required to demonstrate impact on the stroke workflow that result in improved patient outcomes, operational efficiencies, and cost reductions.
Abstract WP79: Combination of Tmax and Relative CBV Perfusion Parameters More Accurately Predicts CTA Collaterals Than a Single Perfusion Parameter in DEFUSE 3
Authors: Mlynash Michael , Lansberg Maarten G , Kemp Stephanie , Christensen Soren , Yennu Amarnath , Heit Jeremy J , Marks Michael P , Albers Gregory W
Published: January 2019

Introduction:
Perfusion abnormalities that have been associated with collateral status include the hypoperfusion intensity ratio (HIR) and reduction of cerebral blood volume within the ischemic lesion (CBV index). We hypothesized that assessment of both of these parameters would predict poor CTA collaterals with greater accuracy than a single parameter. This hypothesis was assessed among patients enrolled in the DEFUSE 3 trial that evaluated the efficacy of thrombectomy in patients with imaging evidence of salvageable tissue in the late 6-16 hour window.

Methods:
Patients were eligible for this analysis if they had adequate quality CTA and CT perfusion. An expert neuroradiologist assessed collateral status on CTA using the Tan scale. The HIR index (fraction of Tmax>10s delay within the Tmax>6s lesion) and the CBV index (mean CBV within the Tmax>6s lesion relative to normal CBV) were automatically calculated by RAPID software. ROC curve analysis was used to identify thresholds for both indices that predicted presence of poor collaterals on CTA. Proportions were compared using χ
2
and McNemar tests.

Results:
Of the 128 cases eligible for this analysis, 26% had poor collaterals on CTA. HIR ≥0.39 and CBV index ≤0.77 were the optimal thresholds for predicting poor collaterals on CTA. Good collaterals were present on CTA in 87% when neither of the perfusion indices were unfavorable, 77% when a single index was unfavorable, and 54% when both were unfavorable (p=0.001 for none vs both and p=0.029 for single vs both). Sensitivity for predicting poor CTA collaterals was 0.70 (95% CI 0.51-0.84) with HIR ≥0.39, 0.61 (0.42-0.77) with CBV index ≤0.77, and 0.82 (0.64-0.92) for the combined criteria of ‘either index unfavorable’ (p=0.016 for ‘either’ criteria vs CBV index alone). The presence of unfavorable criteria on both indices produced a higher specificity of 0.80 (0.70-0.87) vs. HIR alone 0.59 (0.48-0.69) or CBV alone 0.62 (0.52-0.72), both p<0.001.

Conclusion:
The combination of 2 fully automated perfusion parameters were more accurate for predicting CTA collaterals than a single perfusion parameter in the DEFUSE 3 population. Automated perfusion parameters do not require an expert neuroradiologist for interpretation and have no concerns regarding inter-rater agreement.
Abstract WP80: Association of Clot Burden Score and Thrombectomy 6 to 24 Hours After Stroke: Analysis of the DAWN Trial
Authors: Atchaneeyasakul Kunakorn , Liebeskind David S , Yavagal Dileep , Jadhav Ashutosh P , Haussen Diogo C , Budzik Ronald F , Bonafe Alain , Hanel RIcardo A , Ribo Marc , Cognard Christophe , Sila Cathy , Hassan Ameer E , Smith Wade S , Saver Jeffrey , Nogueira Raul G , Jovin Tudor G ,   DAWN Investigators
Published: January 2019

Introduction:
Clot burden score (CBS) is developed to evaluate anterior circulation ischemic stroke thrombus extent. Lower CBS is associated with worse functional outcome in an analysis of MR CLEAN trial without modifying the effect of mechanical thrombectomy within 6 hours after symptoms onset. An analysis of SWIFT PRIME trial showed that stent-retriever thrombectomy successfully recanalize regardless of CBS. However, thrombus that remains stagnant for longer period may be further compressed by the water-hammer effect of the systolic blood pressure, resulting in removal difficulty. We aim to explore relation of CBS with mechanical thrombectomy (MT) in the extended 6-24 hours of the DAWN trial.

Method:
A blinded assessor evaluated CBS in all patients receiving CTA brain in the multicenter randomized DAWN trial for MT in acute large vessel occlusion last known well 6-24 hours (DWI or CTP Assessment with Clinical Mismatch in the Triage of Wake up and Late Presenting Strokes Undergoing Neurointervention). The cohort was divided into low CBS (0-4) and high CBS (5-10) groups. We used ordinal logistic regression for analysis of association with good outcome (mRS≤2), adjusted for significant baseline variables.

Results:
143 patients were included. Low CBS group consisted of 50 patients (29 received MT and 21 received medical management). High CBS group consisted of 93 patients (44 received MT and 49 received medical management). Baseline demographics were similar between groups. Low CBS group showed no difference in the odd of good outcome, adjusted OR 0.58 (0.3-1.14). Analysis of MT cases, Low CBS group showed a strong trend to higher procedure time (81.5±55.2 versus 56.4±35.3 min, p=0.07). There was no association of Low CBS and odds of good outcome (OR 0.9; 0.27-2.92), successful recanalization (OR 0.58; 0.18-1.89), and symptomatic intracranial hemorrhage (OR 1.67, 0.55-5.09).

Conclusion:
Despite literature reporting worse outcome of anterior circulation ischemic stroke with low CBS, acute large vessel occlusion stroke with last known well 6-24 hours and low CBS treated with thrombectomy was feasible and not associated with worsen outcome compared to high CBS. However, a strong trend to higher procedure time is observed.
Spectacular song pattern from the Sicilian Mountains: The new cicada species Cicadetta anapaistica sp. n. (Hemiptera: Cicadidae)
Authors: HERTACH THOMAS
Published: January 2019

Acoustic investigations of Cicadetta montana s. l. have revealed the presence of morphologically cryptic species in the last few years. This work describes the new cicada Cicadetta anapaistica sp. n. which was detected in the Madonie and Nebrodi Mountains (Italy, Sicily). The characteristic and sophisticated song is composed of three phrases, modulated on four typical power levels and three frequency ranges. The song pattern is compared with those of the closely related Cicadetta cerdaniensis and Cicadetta cantilatrix. Quantitative and even qualitative intraspecific differences of the song structure among individuals exist which appear to allow individual-specific recognition in many cases. As in other species of the complex, reliable morphological differences between the new species and others in the complex have not been found. The species is currently only known to be endemic to forest and ecotone habitats in a small mountain range. Because of this limited distribution the species is likely to be vulnerable to habitat and climate changes.
The spider genus Chrysometa (Araneae, Tetragnathidae) from the Pico da Neblina and Serra do Tapirapecó mountains (Amazonas, Brazil): new species, new records, diversity and distribution along two altitudinal gradients
Authors: NOGUEIRA ANDRÉ A. , PENA-BARBOSA JOÃO P. P. , VENTICINQUE EDUARDO M. , BRESCOVIT ANTONIO D.
Published: January 2019

Eight new species of the spider genus Chrysometa Simon, 1894 (Araneae, Tetragnathidae) are described and illustrated. Chrysometa nubigena n. sp., C. waikoxi n. sp., C. petrasierwaldae n. sp., C. santosi n. sp., C. yanomami n. sp., C. candianii n. sp., C. lomanhungae n. sp., and C. saci n. sp. Those species were collected in a study on the diversity of spider communities along altitudinal gradients in Brazilian Amazonia. C. saci was captured at the Serra do Tapirapecó (Barcelos), while all the other species are from the Pico da Neblina (São Gabriel da Cachoeira), the highest mountain in Brazil. We provide new records for C. boraceia, C. flava, C. guttata, C. minuta and C. opulenta, and we describe the male of C. minuta for the first time. We also present the first results on the diversity and altitudinal distribution of the species of Chrysometa at the Pico da Neblina and Serra do Tapirapecó. We sampled the first locality at six different elevations, and obtained 336 specimens distributed in 12 species. Richness and abundance, as well as relative importance peaked at the highest sites sampled (2,000 and 2,400 m). The three most abundant species showed a segregated distribution, being dominant or exclusively distributed in different altitudes. At the Serra do Tapirapecó, sampling at four different elevations up to 1200 m, we only obtained 40 individuals divided in four species, and there was no clear relation to altitude. Most of the new species were found at mid and high altitude sites, while species from lower altitude sites represented widespread species. The comparison with other neotropical spiders inventories highlights the high diversity recorded at Pico da Neblina, which could be assigned to the large environmental variation covered in this work and to the sampling of high-altitude environments. Inventories in the Andean region and other information in the literature also seem to support the association of Chrysometa with high altitude environments.
DNA barcoding is useful for taxonomy: a reply to Ebach
Authors: MITCHELL ANDREW
Published: January 2019

DNA barcoding is all too often derided by taxonomists with little understanding of how far this emerging subdiscipline of systematics has progressed since it was proposed by Hebert et al. (2003). A prime example is Ebach’s factually incorrect and misleading recent correspondence (Ebach 2011). Ebach and I agree on one point: indeed many readers of Zootaxa would have cringed as they read his letter, though perhaps the cause was a tasteless joke. For brevity I will address only three key points he raised about the uses for DNA barcoding.
Differentiation of external morphology of Damaeidae (Acari: Oribatida) in light of the ontogeny of three species
Authors: SENICZAK STANISŁAW , SENICZAK ANNA
Published: January 2019

Morphology of juvenile stages and ontogeny of Damaeus onustus C. L. Koch, 1844, Damaeus clavipes (Hermann, 1804) and Kunstidamaeus tecticola (Michael, 1888) was investigated. The juveniles of these species differ mainly in body shape and size, and shape of some setae on the gastronotum and legs. The nymphs of all species lose centrodorsal setae of the d-series, and carry the exuviae of previous instars, but D. onustus carries also compact humus mass adhering to exuviae, D. clavipes a lot of loose debris, while K. tecticola usually only exuviae. The kind of camouflage is partly determined by the shape of gastronotal setae; in D. onustus these setae are curved ventrally, in D. clavipes are raised, while in K. tecticola are raised, and strongly curved medial. The nymphs of these species, as the nymphs of all other known Damaeidae, have gastronotal cornicle, which connects the exuviae of previous instars to the gastronotum. The cornicle of particular species differs in shape and location on the gastronotum; in some species is located anteromedial to setae la, in the other between setae lm, and in the other yet between setae lp or h 3 . The adults of these species differ mainly in body size, presence and shape of cuticular apophyses on the body, length of some setae on the prodorsum and notogaster, and the number of setae on legs, including dorsal seta d on genua I–III and tibiae I–IV.
A revision of Anenterotrema Stunkard, 1938 (Digenea: Anenterotrematidae) and a key to its species
Authors: LUNASCHI LÍA I. , DRAGO FABIANA B.
Published: January 2019

Anenterotrema Stunkard, 1938 is revised and a key to its recognized species is presented. Anenterotrema auritum Stunkart, 1938 (type–species), A. eduardocaballeroi (Freitas, 1960) Caballero, 1960, A. liliputianum (Travassos, 1928) Caballero, 1964, A. stunkardi Caballero & Grocott, 1959 and A. mesolecitha Marshall & Miller, 1979 are redescribed based on their type material. Anenterotrema singulare Stunkart, 1938 is considered a synonym of A. auritum, A. freitasi Caballero, 1964 and A. auritum of Zdzitowiecki & Rutkowska (1980) synonyms of A. eduardocaballeroi, and A. eduardocaballeroi of Castiblanco & Vélez (1982) conspecific with A. stunkardi.
Taxonomic treatment of the endemic Malagasy praying mantis genus Hyalomantis GIGLIO-TOS, 1915, with a new synonymy and the description of three new species (Mantodea, Iridopterygidae, Tropidomantinae)
Authors: SVENSON GAVIN J. , ROY ROGER
Published: January 2019

The praying mantis genus Hyalomantis Giglio-Tos, 1915 (Mantodea, Iridopterygidae, Tropidomantinae) historically included two species, H. madagascariensis Saussure, 1870 and H. punctata Giglio-Tos, 1915. However, we found that H. punctata is a junior synonym through investigation of the original descriptions and specimen comparisons. We have redescribed H. madagascariensis to encompass variability of color patterns that may have led to the description of H. punctata. The taxonomic history for the genus and species is outlined partly to resolve under Article 33.3.1 of the Code (ICZN, 4 th edition) an incorrect subsequent spelling of Miopteryx madagascarensis Saussure, 1870 that is in prevailing use. In addition, we add three new species to the genus described from Madagascar: H. antsingica n. sp., H. murzini n. sp., and H. whitingi n. sp. A key to the four species in English and French is provided for increase accessibility to the people of Madagascar. Illustrations of major features used to diagnose species are provided including the head, pronotum, and male genitalia. Species distribution is presented and locality coordinates are provided in print as well as being available for download as a KML file viewable in Google Earth.
The immature stages of Tropisternus (Pleurhomus) sahlbergi (Sharp) (Coleoptera: Hydrophilidae), with considerations on the primary chaetotaxy of Tropisternus Soliera
Authors: TORRES PATRICIA L. M. , MICHAT MARIANO C. , ARCHANGELSKY MIGUEL
Published: January 2019

The preimaginal stages of Tropisternus (Pleurhomus) sahlbergi (Sharp, 1883) are described for the first time, including illustrations and photographs of the egg case, all larval instars and pupa. The descriptions emphasize on the morphometry and chaetotaxy, following current standards in larval descriptions within Hydrophilidae. Preliminary evidence is presented for a placement of Pleurhomus Sharp, 1833 within Tropisternus Solier, 1834, in agreement with a recent phylogenetic analysis based on adult characters. Tropisternus (P.) sahlbergi is characterized by a brown to dark brown head capsule without color pattern, mandibles with strongly serrated inner margins, a short seta PA11 in comparison to PA14, the absence of seta AN8, the absence of a distal secondary seta on the outer margin of the first maxillary palpomere and the dorsal surface of the mentum almost entirely covered by spinulae.
Revision of the Holarctic species of Dilyta Förster (Hymenoptera: Figitidae: Charipinae) with descriptions of four new species from the eastern Palaearctic
Authors: PARETAS-MARTÍNEZ JORDI , FERRER-SUAY MAR , KOVALEV OLEG , MELIKA GEORGE , SELFA JESÚS , PUJADE-VILLAR JULI
Published: January 2019

The genus Dilyta, and the known species of this genus present in the Holarctic, D. subclavata Förster from Europe and D. rathmanae Menke and Evenhuis from USA, are redescribed. Four new species of Dilyta are described from the eastern Palaearctic region: D. aleevae sp. n. from Kazakhstan, D. japonica sp. n. from Japan, and D. longinqua sp. n. and D. sinica sp. n. from China. All six species have an ∩ -shaped carina on the apex of the scutellum; this character differentiates these species from the Afrotropical species, which instead have two small symmetrical and parallel carinae. A key to the species of Dilyta in the Holarctic is provided. Apocharips talitzkii (Belizin) is synonymyzed with D. subclavata.
Andraegoidus Aurivillius: new species, synonymies and key to the species (Insecta: Coleoptera: Cerambycidae)
Authors: R. JUAN PABLO BOTERO , MONNÉ MARCELA L.
Published: January 2019

Andraegoidus translucidus sp. nov. is described from Bolivia (Santa Cruz). The following synonyms are proposed: Andraegoidus homoplatus hassenteufeli Fuchs, 1958 = Andraegoidus homoplatus (Dupont, 1838), and Andraegoidus lacordairei punctipennis Hüdepohl, 1985 = Andraegoidus lacordairei (Dupont, 1838). A key to the species of this genus is provided. The geographical distribution of Andraegoidus lacordairei is now widened to Trinidad and Tobago (Talparo).
New records of Tardigrada from Bulgaria with the description of Macrobiotus binieki sp. nov. (Eutardigrada: Macrobiotidae) and a key to the species of the harmsworthi group
Authors: KACZMAREK ŁUKASZ , GOŁDYN BARTŁOMIEJ , PROKOP ZOFIA M. , MICHALCZYK ŁUKASZ
Published: January 2019

Fifteen moss samples collected in the Sophia Province (Bulgaria) were examined. In these samples six eutardigrade species were found: Hypsibius convergens, Isohypsibius prosostomus, Macrobiotus binieki sp. nov., M. hufelandi, M. pallarii and Ramazzottius oberhaeuseri. The new species belongs to the Macrobiotus harmsworthi group and differs from the most similar M. australis, M. coronatus, M. patiens, M. pseudocoronatus, M. radiatus, M. rigidus and M. simulans mainly by: egg processes covered by small bubbles (not smooth or reticulated), egg shell between processes covered by wrinkles not dots or stripes forming a large radiate crown, a higher number of processes on egg circumference and some morphometric characters of adults. In this paper a key to all species of the harmsworthi group is also given.
The Neotropical spider genus Paradossenus (Araneae, Trechaleidae): a new species, taxonomic notes and new records
Authors: DA SILVA ESTEVAM L. CRUZ , LISE ARNO A.
Published: January 2019

The male of Paradossenus pulcher Sierwald, 1993 and a new species, P. macuxi, from Roraima, Northern Brazil are described and illustrated for the first time. The monotypic genus Magnichela Silva & Lise, 2006 is a junior synonym of Paradossenus F.O. Pickard-Cambridge, 1903. Paradossenus amazonensis Carico & Silva, 2010 is a junior synonym of Magnichela santaremensis Silva & Lise, 2006 (type species). New data on the Brazilian distributions of Paradossenus acanthocymbium Carico & Silva, 2010, P. tocantins Carico & Silva, 2010 and P. pozo Carico & Silva, 2010 are presented.
A new spider species Harpactea complicata Deltshev sp. nov. from caves of Serbia (Araneae: Dysderidae)
Authors: DELTSHEV CHRISTO , ĆURČIĆ BOŽIDAR P. M.
Published: January 2019

Dysderid spiders are common in ground habitats, especially leaf litter, and are often found in caves (Chatzaki & Arnedo 2006). The genus Harpactea Bristowe currently includes 5 species in Serbia, i.e. Harpactea hombergi (Scopoli), Harpactea lepida (C.L. Koch), Harpactea pr. incerta Brignoli, Harpactea rubicunda (C.L. Koch), Harpactea saeva (Herman) (Deltshev et al. 2003). They are known from the territory of Europe, widespread mainly in central, southern and eastern parts (Platnick 2011). Harpactea complicata sp. nov. (male/female) is here described and illustrated. The new species was collected in South Western Serbia, Javor Mountain, also in caves. To clear the situation with Harpactea pr. incerta needs additional investigations.
The taxonomy of species assigned to Camptosaurus (Dinosauria: Ornithopoda)
Authors: MCDONALD ANDREW T.
Published: January 2019

The taxonomic affinities of species assigned to Camptosaurus are reassessed in light of firsthand examination of the relevant specimens and a new phylogenetic analysis, the first to incorporate all putative species of Camptosaurus. “Camp- tosaurus” valdensis is found to be an indeterminate dryosaurid. The species C. prestwichii, C. aphanoecetes, and C. depressus differ in several important details from the type species, C. dispar, and are assigned to different genera as Cumnoria prestwichii, Uteodon aphanoecetes gen. et comb. nov., and Osmakasaurus depressus gen. et comb. nov. The recent assignment of O. depressus to the genus Planicoxa is considered unjustified.
The genus Ipodoryctes Granger, 1949 (Hymenoptera: Braconidae, Doryctinae) from China
Authors: TANG PU , ZHU LAN-LAN , HE JUN-HUA , CHEN XUE-XIN
Published: January 2019

The Chinese Ipodoryctes Granger, 1949 from the Parasitic Hymenoptera Collection of Zhejiang University were studied and twelve species recognized, with seven new species proposed, namely I. brevivenus sp. nov., I. guizhouensis sp. nov., I. hebeiensis sp. nov., I. liui sp. nov., I. rugosus sp. nov., I. rutilans sp. nov. and I. wuyiensis sp. nov., and four records new to China, i.e., I. annulicornis Belokobylskij, I. longi Belokobylskij, I. rugosiscutum Belokobylskij, and I. tamdaoensis Belokobylskij. A key to the Chinese species of Ipodoryctes is provided.
Four new species of the subgenus Sinoropeza Alexander, with a key to world species (Diptera, Tipulidae, Dolichopeza)
Authors: LIU QIFEI , YANG DING
Published: January 2019

The following four new species of the subgenus Sinoropeza Alexander from China are described: Dolichopeza (Sinoro- peza) apicalis sp. nov., D. (S.) hamulifera sp. nov., D. (S.) cuspidigera sp. nov. and D. (S.) furcellatusa sp. nov. A key to the world species of the subgenus Sinoropeza is presented.
Prochetostoma expandens (Diptera: Tephritidae) sp. n., a fruit parasite of Ilex integra Thunberg (Aquifoliaceae) in Japan
Authors: SUEYOSHI MASAHIRO , HAN HO-YEON
Published: January 2019

The third species of the genus Prochetostoma, developing in the fruits of Ilex integra Thunberg, has been reported six times in Japan since 1985 but has not been described. We here describe adults and immatures of this species as P. expandens sp. n. Based on the adult morphological characters, P. expandens is suggested to be the sister species of P. bhutanicum Han. Two possible morphological synapomorphies of these two species are proposed. We also investigated a large number of larvae of P. expandens collected from fruits of I. integra. Detailed information about the life history is presented: oviposition takes place under the skin of immature fruits of I. integra in spring; larvae consume fruit tissue in summer and autumn; single fully matured larva escapes from the fruit and drops down to the ground for pupation; and adults emerge in the subsequent spring.
Descriptions of new species of the genus Woznessenskia (Orthoptera: Gryllacrididae: Gryllacridinae) from China
Authors: GUO LI-YING , SHI FU-MING
Published: January 2019

Three new species of the genus Woznessenskia Gorochov, 2002 from China are described in this paper, i.e. W. arcoida sp. nov., W. brevisa sp. nov., W. bimacula sp. nov.. A key to the species of the genus Woznessenskia is provided.
Description of Macutula, a new genus of jumping spiders from Northeastern Brazil (Araneae: Salticidae: Amycoida)
Authors: RUIZ GUSTAVO RODRIGO SANCHES
Published: January 2019

Three new species from Northeastern Brazil are described and included in the newly proposed genus Macutula gen. nov. The type species M. aracoiaba sp. nov. and M. caruaru sp. nov. are described based on specimens from the State of Pernambuco, while M. santana sp. nov. is based on specimens from the State of Bahia.
Type depositories of Chalcidoidea (Hymenoptera) species described from the Department of Zoology, Aligarh Muslim University, India
Authors: HAYAT MOHAMMAD , KHAN F. R. , BADRUDDIN S. M. A.
Published: January 2019

The type specimens of 717 chalcidoid species described by taxonomists from the Department of Zoology, Aligarh Muslim University, India, and their depositories are tabulated. Table 1 lists the holotypes and other type specimens of the species deposited in the Natural History Museum, London, England (BMNH), National Zoological Collections, Zoological Survey of India, Kolkata, India (NZSI), Forest Entomology Division, Forest Research Institute, Dehra Dun, India (FRI), National Pusa Collections, Division of Entomology, Indian Agricultural Research Institute, New Delhi, India (NPC), and the Insect Collection, Department of Zoology, Aligarh Muslim University, Aligarh, India (ZDAMU). The holotypes and lectotypes of 700 species are distributed as follows: BMNH (175), NZSI (34), FRI (28), NPC (131), and ZDAMU (332). The holotypes of 17 species could not be located in ZDAMU, but all of these species are represented by paratypes. A further 23 species whose types are not located in ZDAMU are listed in Table 2.
Two new species of Larsia Fittkau, 1962 (Diptera: Chironomidae: Tanypodinae) from Neotropical Region, with a checklist of Larsia species of the world
Authors: DE OLIVEIRA CAROLINE SILVA NEUBERN , DA SILVA FABIO LAURINDO
Published: January 2019

Two new species of Larsia Fittkau, 1962 are described and figured as male, pupa and larva. Diagnosis to male of this genus is emended. An annotated checklist of the world’s valid Larsia species is given and keys to pupae and larvae of known species in the Neotropical region are provided.
A taxonomic study on some Alpine Hypogastrura Bourlet, 1839 (Collembola, Hypogastruridae)
Authors: SKARŻYŃSKI DARIUSZ
Published: January 2019

The examination of the holotypes of Hypogastrura parvula Haybach, 1972 and Orogastrura parva (Gisin, 1949) revealed that they are conspecific, resulting in the synonymization of the former with the latter. The similar, but poorly known, species H. exigua Gisin, 1958 and H. oreophila Butschek, 1948 from the Alps have been redescribed.
Cranial anatomy of tadpoles of five species of Scinax (Hylidae, Hylinae)
Authors: ALCALDE LEANDRO , CANDIOTI FLORENCIA VERA , KOLENC FRANCISCO , BORTEIRO CLAUDIO , BALDO DIEGO
Published: January 2019

We studied the oral apparatus, buccal cavity and musculoskeletal features in tadpoles of five species of the genus Scinax (S. acuminatus, S. uruguayus, S. aff. pinima, S. aromothyella, and S. berthae). Observed variation is mainly related to intrageneric grouping. Scinax acuminatus (S. ruber clade, sister taxon of S. rostratus group) has a distinctive combination of a mental gap in the margin of oral papillae, straight labial teeth with few or absent cusps, processus muscularis acute and posteriorly directed, and m. subarcualis rectus I with two slips. Scinax uruguayus and S. aff. pinima (S. uruguayus group) have keratinized sheets ventrolateral to the lower jaw sheath, well-developed infralabial and lateral ridge papillae, robust jaw cartilages, cornua trabeculae with short and widely divergent free portions, processus articularis short and wide, processus muscularis thin and directed anteriorly. Scinax aromothyella and S. berthae (S. catharinae group) have poorly developed, non-colored spurs behind the lower jaw sheath, long and thin processus articularis, wide and rounded processus muscularis, and tripartite cartilago suprarostralis. Anatomical features described are congruent with current phylogenetic arrangements based on molecular, chromosomal, and morphological data, and provide a source of information that can be useful to solve interspecific relationships within Scinax.
Review of eastern Asian species of the mysid genus Rhopalophthalmus Illig, 1906 (Crustacea, Mysida) with descriptions of three new species
Authors: HANAMURA YUKIO , MURANO MASAAKI , MAN ALIAS
Published: January 2019

Mysid crustaceans of the genus Rhopalophthalmus Illig, 1906 (Crustacea: Mysida) are distributed in the eastern Atlantic through the Indo-West Pacific. In this paper, species of Rhopalophthalmus recorded from eastern Asian waters are reviewed on the basis of materials collected from south-eastern Asia to Japanese water. The taxonomic analysis led to the recognition of six species from the region, including three new species. Rhopalophthalmus phyllodus Murano, 1988 was regarded as a junior synonym of R. egregius Hansen, 1910. The identity of R. macropsis Pillai, 1964 has been controversial, and in this study, we recognised it as valid. However, specimens with remarkably stout and few spinose setae on the telson, often reported as either R. macropsis or R. longipes Ii, 1964 were determined to be a new species, R. armiger. In addition, two new species, R. hastatus and R. philippinensis, were reported from south-eastern Asian waters. A key to the 25 currently recognised world species is provided.
A new species of Liolaemus of the Liolaemus montanus section (Iguania: Liolaemidae) from Northwestern Argentina
Authors: QUINTEROS ANDRÉS SEBASTIÁN , ABDALA CRISTIAN SIMÓN
Published: January 2019

We provide evidence for a new species of Liolaemus, which can be included in the L. dorbignyi group within the montanus series. Liolaemus vulcanus sp. nov. was previously confused with L. dorbignyi, but it exhibits states of character which allow us to differentiate the two taxa. The main differences with L. dorbignyi are in the color pattern and in the dorsal scales. Liolaemus vulcanus sp. nov. is saxicolous and it inhabits in rocky hills in the Puna regions of Northwestern Argentina. It is distributed in localities in Antofagasta de la Sierra department in Catamarca Province, Argentina .
Two Northeast Pacific deep-water barnacle populations (Cirripedia: Calanticidae and Pachylasmatidae) from seamounts of the Juan de Fuca Ridge; "insular" endemics stemming from Tethys, or by subsequent dispersal from the Western Pacific center of distribution?
Authors: NEWMAN WILLIAM A. , JONES WILLIAM J.
Published: January 2019

The first adults of the calanticid, Calantica moskalevi Zevina and Galkin, 1989, and specimens of a new pachylasmatine balanomorph genus and species, have been recovered by MBARI’s ROV Tiburon, from Juan de Fuca Ridge seamounts at ~46º N – 130º W in the NE Pacific off Oregon, ~1450 m and 2080 m depths, respectively. These two apparently allopathic populations evidently represent remnants of stocks most commonly confined to relatively deep waters around islands and occasionally continental margins of the Indo-West Pacific. These Juan de Fuca representatives can be inferred to be relicts of once broad Paleogene Tethyan populations rather than relatively recent immigrants by way of the NW Pacific. Apparently, the refugium afforded by seamount “islands” at bathyal and abyssal depths accounts for their survival in this relatively remote corner of Pacific Oceania.
A new species of Palpares Rambur (Neuroptera: Myrmeleontidae) with an identification key to the species of West Africa
Authors: AKOUDJIN MASSOUROUDINI , MICHEL BRUNO
Published: January 2019

Palpares longimaculatus nov. sp. is described from West Africa. This species resembles P. radiatus Rambur, 1842 and P. incommodus (Walker, 1853) from the same region and is very similar to P. kalahariensis Stitz, 1912 from South Africa. The characters differentiating these species are indicated. An identification key to the West African species of Palpares is provided and all the keyed species are illustrated.
Three new species and a new key to the Diplonevra Lioy (Diptera: Phoridae) from Australia
Authors: DISNEY R. H. L.
Published: January 2019

Diplonevra collessi sp. nov., D. fumosa sp. nov. and D. nigricorpus sp. nov. are described from Australia. A key to the eleven Australian species known in the male sex is provided.