bluuebunny/crossref_metadata_2025 huggingface dataset:
(Mis)Information, Fears and Preventative Health Behaviours Related to COVID-19
Authors: Castellano-Tejedor Carmina , Torres-Serrano María , Cencerrado Andrés
Published: April 2022

Social and mass media platforms (SMM) are essential tools for keeping people informed about health-promoting practices. However, the potential to spread misinformation or false rumors exists. These might influence preventive health behaviours and incite anxiety and/or fear among the population. A sample of 300 adults participated in a survey to understand information needs, fears and preventive health behaviours related to COVID-19 while analyzing differences in COVID-19 acceptance rates. Descriptive-correlational, between-group comparisons and regression analyses were applied. Most of the sample revealed a willingness to accept COVID-19 vaccines (65.4% vs. 34.5%) and was prone to use and trust different SMM without experiencing significant obstacles in managing COVID-19-related information except for the need to ration it from time to time (χ2(2, N = 298) = 6.654, p = 0.036). Preventive behaviours/measures carried out were similar among the people resistant, hesitant or willing to get vaccinated for COVID-19. However, higher self-efficacy was observed in resistant vaccine individuals (F(2) = 3.163, p = 0.044). Psychological impact (need for psychological support due to COVID-19 situation) in accepting (F(5, 189) = 17.539, p < 0.001, R2 = 0.317) and hesitant individuals (F(5, 77) = 17.080, p < 0.001, R2 = 0.526) was explained by female gender, younger age, threat susceptibility and differential characteristics in terms of psychological symptoms experienced and SMM trust. No explanatory model was obtained for the resistant individuals. SMM could be effective tools to promote COVID-19 health preventive behaviours. However, psychographic characteristics might modulate information-seeking and management as well as self-perceived threat susceptibility and severity. All these factors must be accurately considered when designing different health preventive campaigns for the general public.
Projecting the Pandemic Trajectory through Modeling the Transmission Dynamics of COVID-19
Authors: Vakil Vahideh , Trappe Wade
Published: April 2022

The course of the COVID-19 pandemic has given rise to many disease trends at various population scales, ranging from local to global. Understanding these trends and the epidemiological phenomena that lead to the changing dynamics associated with disease progression is critical for public health officials and the global community to rein in further spread of this and other virulent diseases. Classic epidemiological modeling based on dynamical systems are powerful tools used for modeling and understanding diseases, but often necessitate modifications to the classic compartmental models to reflect empirical observations. In this paper, we present a collection of extensions to the classic SIRS model to support public health decisions associated with viral pandemics. Specifically, we present models that reflect different levels of disease severity among infected individuals, capture the effect of vaccination on different population groups, capture the effect of different vaccines with different levels of effectiveness, and model the impact of a vaccine with varying number of doses. Further, our mathematical models support the investigation of a pandemic’s trend under the emergence of new variants and the associated reduction in vaccine effectiveness. Our models are supported through numerical simulations, which we use to illustrate phenomena that have been observed in the COVID-19 pandemic. Our findings also confirm observations that the mild infectious group accounts for the majority of infected individuals, and that prompt immunization results in weaker pandemic waves across all levels of infection as well as a lower number of disease-caused deaths. Finally, using our models, we demonstrate that, when dealing with a single variant and having access to a highly effective vaccine, a three-dose vaccine has a strong ability to reduce the infectious population. However, when a new variant with higher transmissibility and lower vaccine efficiency emerges, it becomes the dominant circulating variant, as was observed in the recent emergence of the Omicron variant.
Biomarker of Neuroinflammation in Parkinson’s Disease
Authors: Liu Tsai-Wei , Chen Chiung-Mei , Chang Kuo-Hsuan
Published: April 2022

Parkinson’s disease (PD) is caused by abnormal accumulation of α-synuclein in dopaminergic neurons of the substantia nigra, which subsequently causes motor symptoms. Neuroinflammation plays a vital role in the pathogenesis of neurodegeneration in PD. This neuroinflammatory neurodegeneration involves the activation of microglia, upregulation of proinflammatory factors, and gut microbiota. In this review, we summarized the recent findings on detection of PD by using inflammatory biomarkers, such as interleukin (IL)-1β, IL-2, IL-6, IL-10, tumor necrosis factor (TNF)-α; regulated upon activation, normal T cell expressed and presumably secreted (RANTES) and high-sensitivity c-reactive protein (hsCRP); and radiotracers such as [11C]PK11195 and [18F]-FEPPA, as well as by monitoring disease progression and the treatment response. Many PD-causing mutations in SNCA, LRRK2, PRKN, PINK1, and DJ-1 are also associated with neuroinflammation. Several anti-inflammatory medications, including nonsteroidal anti-inflammatory drugs (NSAID), inhibitors of TNF-α and NLR family pyrin domain containing 3 (NLRP3), agonists of nuclear factor erythroid 2-related factor 2 (NRF2), peroxisome proliferator-activated receptor gamma (PPAR-γ), and steroids, have demonstrated neuroprotective effects in in vivo or in vitro PD models. Clinical trials applying objective biomarkers are required to investigate the therapeutic potential of anti-inflammatory medications for PD.
Replicative Stress Coincides with Impaired Nuclear DNA Damage Response in COX4-1 Deficiency
Authors: Douiev Liza , Miller Chaya , Keller Guy , Benyamini Hadar , Abu-Libdeh Bassam , Saada Ann
Published: April 2022

Cytochrome c oxidase (COX), a multimeric protein complex, is the final electron acceptor in the mitochondrial electron transfer chain. Primary COX deficiency, caused by mutations in either mitochondrial DNA or nuclear-encoded genes, is a heterogenous group of mitochondrial diseases with a wide range of presentations, ranging from fatal infantile to subtler. We previously reported a patient with primary COX deficiency due to a pathogenic variant in COX4I1 (encoding the common isoform of COX subunit 4, COX4-1), who presented with bone marrow failure, genomic instability, and short stature, mimicking Fanconi anemia (FA). In the present study, we demonstrated that accumulative DNA damage coincided primarily with proliferative cells in the patient’s fibroblasts and in COX4i1 knockdown cells. Expression analysis implicated a reduction in DNA damage response pathways, which was verified by demonstrating impaired recovery from genotoxic insult and decreased DNA repair. The premature senescence of the COX4-1-deficient cells prevented us from undertaking additional studies; nevertheless, taken together, our results indicate replicative stress and impaired nuclear DNA damage response in COX4-1 deficiency. Interestingly, our in vitro findings recapitulated the patient’s presentation and present status.
Cerebroventricular Injection of Pgk1 Attenuates MPTP-Induced Neuronal Toxicity in Dopaminergic Cells in Zebrafish Brain in a Glycolysis-Independent Manner
Authors: Lin Cheng-Yung , Tseng Hsiang-Chien , Chu Yu-Rong , Wu Chia-Lun , Zhang Po-Hsiang , Tsai Huai-Jen
Published: April 2022

Parkinson’s disease (PD) is characterized by the degeneration of dopaminergic neurons. While extracellular Pgk1 (ePgk1) is reported to promote neurite outgrowth, it remains unclear if it can affect the survival of dopaminergic cells. To address this, we employed cerebroventricular microinjection (CVMI) to deliver Pgk1 into the brain of larvae and adult zebrafish treated with methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) as a PD-like model. The number of dopamine-producing cells in ventral diencephalon clusters of Pgk1-injected, MPTP-treated embryos increased over that of MPTP-treated embryos. Swimming distances of Pgk1-injected, MPTP-treated larvae and adult zebrafish were much longer compared to MPTP-treated samples. The effect of injected Pgk1 on both dopamine-producing cells and locomotion was time- and dose-dependent. Indeed, injected Pgk1 could be detected, located on dopamine neurons. When the glycolytic mutant Pgk1, Pgk1-T378P, was injected into the brain of MPTP-treated zebrafish groups, the protective ability of dopaminergic neurons did not differ from that of normal Pgk1. Therefore, ePgk1 is functionally independent from intracellular Pgk1 serving as an energy supplier. Furthermore, when Pgk1 was added to the culture medium for culturing dopamine-like SH-SY5Y cells, it could reduce the ROS pathway and apoptosis caused by the neurotoxin MPP+. These results show that ePgk1 benefits the survival of dopamine-producing cells and decreases neurotoxin damage.
Antiphospholipid Antibodies and Lipids in Hematological Malignancies
Authors: Barreno-Rocha Sonia Guadalupe , Guzmán-Silahua Sandra , Rodríguez-Dávila Sinaí-del-Carmen , Gavilanez-Chávez Guadalupe Estela , Cardona-Muñoz Ernesto Germán , Riebeling-Navarro Carlos , Rubio-Jurado Benjamín , Nava-Zavala Arnulfo Hernán
Published: April 2022

One of the main groups of lipids is phospholipids, which are mainly involved in forming cell membranes. Neoplastic processes such as cell replication have increased lipid synthesis, making tumor cells dependent on this synthesis to maintain their requirements. Antiphospholipid antibodies attack phospholipids in the cell membranes. Three main types of antiphospholipid antibodies are recognized: anti-β2 glycoprotein I (anti-β2GP-I), anticardiolipin (aCL), and lupus anticoagulant (LA). These types of antibodies have been proven to be present in hematological neoplasms, particularly in LH and NHL. This review on antiphospholipid antibodies in hematological neoplasms describes their clinical relationship as future implications at the prognostic level for survival and even treatment.
SARS-CoV-2 Pandemic Tracing in Italy Highlights Lineages with Mutational Burden in Growing Subsets
Authors: Boccia Angelo , Tufano Rossella , Ferrucci Veronica , Sepe Leandra , Bianchi Martina , Pascarella Stefano , Zollo Massimo , Paolella Giovanni
Published: April 2022

Tracing the appearance and evolution of virus variants is essential in the management of the COVID-19 pandemic. Here, we focus on SARS-CoV-2 spread in Italian patients by using viral sequences deposited in public databases and a tracing procedure which is used to monitor the evolution of the pandemic and detect the spreading, within the infected population of emergent sub-clades with a potential positive selection. Analyses of a collection of monthly samples focused on Italy highlighted the appearance and evolution of all the main viral sub-trees emerging at the end of the first year of the pandemic. It also identified additional expanding subpopulations which spread during the second year (i.e., 2021). Three-dimensional (3D) modelling of the main amino acid changes in mutated viral proteins, including ORF1ab (nsp3, nsp4, 2’-o-ribose methyltransferase, nsp6, helicase, nsp12 [RdRp]), N, ORF3a, ORF8, and spike proteins, shows the potential of the analysed structural variations to result in epistatic modulation and positive/negative selection pressure. These analyzes will be of importance to the early identification of emerging clades, which can develop into new “variants of concern” (i.e., VOC). These analyses and settings will also help SARS-CoV-2 coronet genomic centers in other countries to trace emerging worldwide variants.
Development of Home Mechanical Ventilation in Poland in 2009–2019 Based on the Data of the National Health Fund
Authors: Czajkowska-Malinowska Małgorzata , Bartolik Kinga , Nasiłowski Jacek , Kania Aleksander
Published: April 2022

Home mechanical ventilation (HMV) is a dynamically developing field of medicine driven by the increasing number of patients and technological advancements. In Poland, HMV has been financed from public funds since 2004. However, the organization of HMV is still evolving in search of the optimal model of care. The aim of this study was to analyze 11 years of HMV in terms of the number of patients, modes of ventilation, diagnosis and regional prevalence. In retrospective analysis of data reported to the National Health Fund by all health entities providing HMV in Poland in the period from 2009 to 2019, the following variables were included: age, sex, date of commencement, ventilation mode, diagnosis, and place of treatment. The diseases were identified according to the ICD-10 codes. A total of 12,616 patients receiving HMV were reported, including 1221 children (9.7%). The HMV prevalence increased from 2.8 in 2009 to 20/100,000 in 2019. In adults, the highest increase was reported for patients with chronic obstructive pulmonary disease, who accounted for 39% of all HMV users in 2019. The proportion of noninvasive ventilation (NIV) increased from 56% in 2014 to 73% in 2019. We identified significant regional variations in the prevalence of HMV between provinces. The main drivers for HMV development include full reimbursement, the development of hospital NIV centers and the involvement of respiratory physicians in the referral process for HMV.
Green Bioprinting with Layer-by-Layer Photo-Crosslinking: A Designed Experimental Investigation on Shape Fidelity and Cell Viability of Printed Constructs
Authors: Thakare Ketan , Jerpseth Laura , Pei Zhijian , Qin Hongmin
Published: April 2022

Process variables of bioprinting (including extrusion pressure, nozzle size, and bioink composition) can affect the shape fidelity and cell viability of printed constructs. Reported studies show that increasing extrusion pressure or decreasing nozzle size would decrease cell viability in printed constructs. However, a smaller nozzle size is often necessary for printing constructs of higher shape fidelity, and a higher extrusion pressure is usually needed to extrude bioink through nozzles with a smaller diameter. Because values of printing process variables that increase shape fidelity can be detrimental to cell viability, the optimum combination of variables regarding both shape fidelity and cell viability must be determined for specific bioink compositions. This paper reports a designed experimental investigation (full factorial design with three variables and two levels) on bioprinting by applying layer-by-layer photo-crosslinking and using the alginate-methylcellulose-GelMA bioink containing algae cells. The study investigates both the main effects and interaction effects of extrusion pressure, nozzle size, and bioink composition on the shape fidelity and cell viability of printed constructs. Results show that, as extrusion pressure changed from its low level to its high level, shape fidelity and cell viability decreased. As nozzle size changed from its low level to its high level, shape fidelity decreased while cell viability increased. As bioink composition changed from its low level (with more methylcellulose content in the bioink) to its high level (with less methylcellulose content in the bioink), shape fidelity and cell viability increased.
Biological Effects of Non-Ionizing Electromagnetic Fields at 27 GHz on Sperm Quality of Mytilus galloprovincialis
Authors: Pecoraro Roberta , Pavone Santi Concetto , Scalisi Elena Maria , Sica Carmen , Ignoto Sara , Contino Martina , Salvaggio Antonio , Marmara Dimitra , Sorbello Gino , Di Donato Loreto , Brundo Maria Violetta
Published: April 2022

Recently, an increasing use of wireless internet technologies has been demonstrated. The devices which use these technologies emit in new spectral regions an electromagnetic radiation (EMF) which could interact with the male reproductive system. The aim of this study was to investigate in vitro the effect of electromagnetic fields at 27 GHz on sperm quality in Mytilus galloprovincialis. Sperm samples were collected from sexually mature males of M. galloprovincialis and placed in seawater. Once we evaluated the number and quality of spermatozoa, sperm cells were exposed to electromagnetic fields radiated by a pyramidal horn antenna. The effect of exposure was evaluated after 10, 20, 30, 40 and 60 min by a light microscope and using an Eosin test. Ten replications were performed for each time series, and statistical analysis was carried out by t-test. Sperm motility decreased after 10 min of exposure, and after 30 min most of the spermatozoa were immobile and not vital. This study provides useful data on the potential ecological impact of the high-band 5G on animal fertility, the effect of which is currently under investigation.
A Measurement System to Monitor Propulsion Performance and Ice-Induced Shaftline Dynamic Response of Icebreakers
Authors: Zambon Alessandro , Moro Lorenzo , Brown Jeffrey , Kennedy Allison , Oldford Dan
Published: April 2022

Polar navigation entails challenges that affect the continuation of ship operations in severe ice conditions. Due to ice-propeller interaction, propulsion shafting segments are often at a high risk of failure. Efficient methods for shaft line design are hence needed to ensure the safety of ice-going vessels and propulsion reliability. To this end, full-scale measurements have proven essential to support the development of ship-design tools and updated safety regulations for ice-going vessels. This paper presents a unique integrated measurement system that employs measuring equipment to monitor Polar-Class vessel performance and shaft line dynamics during ice navigation. The system was installed on board the Canadian Coast Guard (CCG) icebreaker Henry Larsen. This experimental concept aims to monitor the shaft’s torque and thrust fluctuations during ice navigation to obtain information about the ship’s propulsion efficiency. In the paper, we describe the arrangement of the measurement system and the components it features. Finally, we present preliminary datasets acquired during two icebreaking expeditions. This work is framed into a broader research project, which includes the long-term objective to determine a correlation between sea ice conditions and the dynamic response of shaft lines.
Adaptive Response of Saccharomyces Hosts to Totiviridae L-A dsRNA Viruses Is Achieved through Intrinsically Balanced Action of Targeted Transcription Factors
Authors: Ravoitytė Bazilė , Lukša Juliana , Wellinger Ralf Erik , Serva Saulius , Servienė Elena
Published: April 2022

Totiviridae L-A virus is a widespread yeast dsRNA virus. The persistence of the L-A virus alone appears to be symptomless, but the concomitant presence of a satellite M virus provides a killer trait for the host cell. The presence of L-A dsRNA is common in laboratory, industrial, and wild yeasts, but little is known about the impact of the L-A virus on the host’s gene expression. In this work, based on high-throughput RNA sequencing data analysis, the impact of the L-A virus on whole-genome expression in three different Saccharomyces paradoxus and S. cerevisiae host strains was analyzed. In the presence of the L-A virus, moderate alterations in gene expression were detected, with the least impact on respiration-deficient cells. Remarkably, the transcriptional adaptation of essential genes was limited to genes involved in ribosome biogenesis. Transcriptional responses to L-A maintenance were, nevertheless, similar to those induced upon stress or nutrient availability. Based on these data, we further dissected yeast transcriptional regulators that, in turn, modulate the cellular L-A dsRNA levels. Our findings point to totivirus-driven fine-tuning of the transcriptional landscape in yeasts and uncover signaling pathways employed by dsRNA viruses to establish the stable, yet allegedly profitless, viral infection of fungi.
COVID-19 Impact on the Sport Sector Economy and Athletic Performance
Authors: Wiltshire Huw D. , Supriya Rashmi , Baker Julien S.
Published: April 2022

As COVID-19 continues to impact global health, and educational, financial, commercial institutions, sport, in particular, has not been spared [...]
A Vision on a UNESCO Global Geopark at the Southeastern Dead Sea in Jordan—Geosites and Conceptual Approach
Authors: Al-Halbouni Djamil , AlRabayah Osama , Rüpke Lars
Published: April 2022

A vision for the establishment of a Geopark in Jordan is given in this work, with a subsequent application to the UNESCO Global Geopark programme. The Dead Sea area and its surroundings have suffered strong changes in the last decades, accompanied by a variety of natural hazards related to enhanced erosional processes. The aspiring Geopark will thematically encompass the influence that these changes and related natural hazards, including flash floods and subsidence, have had on the local population, from geological, over historical up to recent times. The hydrogeology and geomorphology, i.e., the connection between erosion by water, dissolution of minerals, and landscape evolution, will be the main guiding theme that connects the Eastern Rim Highlands with the Dead Sea rift valley through ephemeral wadis, vegetated springs areas, and traditionally communities. The creation of the Geopark is aimed at holistic, sustainable development and management of the area by eco-tourism, and includes education on water resource management, hazard awareness and resilience, as well as international research. We here present the conceptual approach to the initial development of a Geopark network in Jordan. In a narrative discourse, we highlight realised and further implementation steps, with an evaluation of the expected timeline, potential partner institutions, regional involvement and the chances for realisation.
The Bighorn Habitat Assessment Tool: A Method to Quantify Conservation Value on Landscapes Impacted by Mining
Authors: Anderson Dayan J. , Bleich Vernon C. , Villepique Jeffrey T.
Published: April 2022

We present a methodology to assess the conservation value of mitigation lands for desert bighorn sheep (Ovis canadensis nelsoni) within landscapes impacted by historic and ongoing industrial uses. The Bighorn Habitat Assessment Tool (BHAT) was developed to support the adaptive management of the Cushenbury population of bighorn sheep located on the north slope of the San Bernardino Mountains in southern California, USA. We use a novel formulation of conservation value integrating the results of resource selection function analysis and reclamation credits, reflecting the degree to which degraded habitat is enhanced to benefit wild sheep. Our method seeks to balance conservation objectives simultaneously with the economic development of a working mine landscape. Specifically, the BHAT can be used to (a) establish a habitat reserve providing maximum benefit to the unique requirements of bighorn sheep; (b) incentivize voluntary action by industry to ensure mining activities are compatible with conservation; (c) allow for the evaluation of multiple mine planning and resource management alternatives; and (d) ensure that future compensatory mitigation actions for mining activity are grounded in the best available science. Our methodology is transferrable to the management of other wild sheep populations occupying mine-influenced landscapes for which sufficient data are available to complete resource selection analyses.
Wetland Construction, Restoration, and Integration: A Comparative Review
Authors: Spieles Douglas J.
Published: April 2022

In response to the global loss and degradation of wetland ecosystems, extensive efforts have been made to reestablish wetland habitat and function in landscapes where they once existed. The reintroduction of wetland ecosystem services has largely occurred in two categories: constructed wetlands (CW) for wastewater treatment, and restored wetlands (RW) for the renewal or creation of multiple ecosystem services. This is the first review to compare the objectives, design, performance, and management of CW and RW, and to assess the status of efforts to combine CW and RW as Integrated Constructed Wetlands (ICW). These wetland systems are assessed for their ecological attributes and their relative contribution to ecosystem services. CW are designed to process a wide variety of wastewaters using surface, subsurface, or hybrid treatment systems. Designed and maintained within narrow hydrologic parameters, CW can be highly effective at contaminant transformation, remediation, and sequestration. The ecosystem services provided by CW are limited by their status as high-stress, successionally arrested systems with low landscape connectivity and an effective lifespan. RW are typically situated and designed for a greater degree of connection with regional ecosystems. After construction, revegetation, and early successional management, RW are intended as self-maintaining ecosystems. This affords RW a broader range of ecosystem services than CW, though RW system performance can be highly variable and subject to invasive species and landscape-level stressors. Where the spatial and biogeochemical contexts are favorable, ICW present the opportunity to couple CW and RW functions, thereby enhancing the replacement of wetland services on the landscape.
Woodland Management as Major Energy Supply during the Early Industrialization: A Multiproxy Analysis in the Northwest European Lowlands
Authors: Oliveira Cláudia , Bouquerel Jonathan , Rochel Xavier , Karimi-Moayed Nasrin , Vandenberghe Dimitri , De Grave Johan , Deforce Koen , Devin Simon , Robin Vincent
Published: April 2022

Wood and charcoal were key sources of energy during early industrialization in Europe (18th century), preceding the large-scale exploitation of fossil coal. Past timber harvesting implied land transformation and woodland resources management. Therefore, relict charcoal kilns and historical documents of forest management are important sources of information about past woodland composition and structure. However, ancient charcoal kilns are poorly documented in temperate woodlands in the lowlands of western Europe, especially combined with historical written sources. In this study, charcoal production was investigated in an area in NE France, by combining charcoal and historical sources analysis, along with innovative dating methodologies. Thus, by using both radiocarbon and optically-stimulated luminescence dating, we showed that the activity lasted until recent times (19th–early 20th centuries) and Carpinus was the dominant taxon in charcoal assemblage. Moreover, kiln attributes seemed to be independent of topographical variables. Woodlands in this area were subject to a coppice-with-standards management, where small diameter wood was preferred to produce charcoal and large diameter stems, mainly Quercus and Fagus, were traded as timber. The dominance of Carpinus is rather uncommon in charcoal studies but supports the importance of Carpinus as fuelwood since the Middle Ages, as confirmed by many written sources.
Spatiotemporal Characteristics of Drought and Wet Events and Their Impacts on Agriculture in the Yellow River Basin
Authors: Li Qingqing , Cao Yanping , Miao Shuling , Huang Xinhe
Published: April 2022

Droughts and floods have proven to be threats to food security worldwide. This research used the standardized precipitation index (SPI) to examine the spatiotemporal characteristics of drought and wet events from 1961 to 2020 in the Yellow River basin (YRB). Grain yield data were combined to assess how drought and wet frequency have affected the agricultural system. The occurrence frequency of drought was greater than that of wetness in time, drought frequency decreased, and wetness increased. Spatially, the frequency of drought in all provinces except Shanxi was higher than that of wetness. The grain yield per unit area of the YRB was generally highest in Shandong province and lowest in Gansu province. The grain yield per unit area have shown a significant growth trend in the nine provinces of the YRB since 1961. Drought had a negative effect on the grain yield per unit area in each province, while wetness had a positive effect on the grain yield per unit area in all provinces except Shandong. In general, the influence of drought on grain yield per unit area decreased, while the influence of wetness on grain yield per unit area increased. The results indicate that human activities are effective against preventing and controlling drought and wet disasters and can provide a reference for other parts of the world.
RNF213-Associated Vascular Disease: A Concept Unifying Various Vasculopathies
Authors: Hiraide Takahiro , Suzuki Hisato , Momoi Mizuki , Shinya Yoshiki , Fukuda Keiichi , Kosaki Kenjiro , Kataoka Masaharu
Published: April 2022

The ring finger protein 213 gene (RNF213) encodes a 590 kDa protein that is thought to be involved in angiogenesis. This gene was first recognized as a vasculopathy-susceptibility locus through genome-wide association studies undertaken in a Japanese population, demonstrating that heterozygotes for RNF213 p.Arg4810Lys (c.14429G>A, rs112735431) had a greatly increased risk of moyamoya disease. The association of RNF213 p.Arg4810Lys as a susceptibility variant of moyamoya disease was reproduced in Korean and Chinese individuals and, later, in Caucasians. Variants of the RNF213 gene have been linked to a number of vascular diseases such as moyamoya disease, intracranial major artery stenosis, pulmonary arterial hypertension, and peripheral pulmonary artery stenosis, and have also been associated with co-occurrent diseases and vascular disease in different organs. Based on the findings that we have reported to date, our paper proposes a new concept of “RNF213-associated vascular disease” to unify these conditions with the aim of capturing patients with multiple diseases but with a common genetic background. This concept will be highly desirable for clarifying all of the diseases in the RNF213-associated vascular disease category by means of global epidemiological investigations because of the possibility of such diseases appearing asymptomatically in some patients.
KCNH2 p.Gly262AlafsTer98: A New Threatening Variant Associated with Long QT Syndrome in a Spanish Cohort
Authors: Lorca Rebeca , Junco-Vicente Alejandro , Pérez-Pérez Alicia , Pascual Isaac , Persia-Paulino Yvan Rafael , González-Urbistondo Francisco , Cuesta-Llavona Elías , Fernández-Barrio Bárbara C. , Morís César , Rubín José Manuel , Coto Eliecer , Gómez Juan , Reguero José Julián Rodríguez
Published: April 2022

Long QT syndrome (LQTS) is an inherited (autosomal dominant) channelopathy associated with susceptibility to ventricular arrhythmias due to malfunction of ion channels in cardiomyocytes, that could lead to sudden death (SD). Most pathogenic variants are in the main 3 genes: KCNQ1 (LQT1), KCNH2 (LQT2) and SCN5A (LQT3). Efforts to improve the understanding of the genotype-phenotype relationship are essential to improve the medical clinical practice. In this study, we identified all index patients referred for NGS genetic sequencing due to LQTS, in a Spanish cohort, who were carriers of a new pathogenic variant (KCNH2 p.Gly262AlafsTer98). Genetic and clinical family screening was performed in order to describe its phenotypic characteristics. We identified 22 relatives of Romani ethnicity, who were carriers of the variant. Penetrance reached a 100% and adherence to medical treatment was low. There was a high rate of clinical events, particularly arrhythmic events and SD (1 in every 4 patients presented syncope, 1 presented an aborted SD, 2 obligated carriers suffered SD before the age of 40 and 4 out of 6 carriers of an implantable cardioverter-defibrillator (ICD) had appropriate ICD therapies. Correct adherence to medical treatment in all carriers should be specially encouraged in this population. ICD implantation decision in non-compliant patients, and refusing left cardiac sympathetic denervation, should be carefully outweighed.
The Green Microalga Coelastrella thermophila var. globulina (Scenedesmaceae, Chlorophyta) Isolated from an Algerian Hot Spring as a Potential Source of Fatty Acids
Authors: Boutarfa Soumia , Senoussi Mohammed Mourad , Gonzalez-Silvera Daniel , López-Jiménez José Ángel , Aboal Marina
Published: April 2022

Screening microalgae from extreme environments, including hot springs, is an important research topic that has lately emerged. A thermophilic green alga was isolated from a north-eastern Algerian hot spring at a temperature of 63 °C, and its fatty acid (FA) profile was explored. The strain was cultivated in BBM medium at 35 °C in a 16:8 h light/dark cycle and 75 μM photons m−2 s−1. The morphological studies combined with phylogenetic analysis revealed that the isolate was Coelastrella thermophila var. globulina Q. Wang, H. Song, X. Liu, G. Liu and Z. Hu. The monounsaturated fatty acid (MUFA) content was 51.12%. The saturated fatty acid (SFA) and polyunsaturated fatty acid (PUFA) content, respectively, accounted for 27.01% and 21.87%. The main FA was oleic acid (18:1n–9), whose value was 35.95%, followed in decreasing order by palmitic acid (16:0) with 21.45%, linoleic acid (18:2n–6) with 14.38% and α-linolenic acid (18:3n–3) with 04.22%. The FA profile exhibited high total n–6 and n–3 PUFA values (15.80% and 5.76%, respectively). Coelastrella thermophila var. globulina is particularly interesting for producing n-6 and n-3 PUFA and is likely suitable for other biotechnological purposes. This is the first time that this taxon has been reported in hot springs. Other species can be expected to be reported, which emphasises the importance of the biodiversity of extreme habitats.
CAR-T Cells for the Treatment of Lung Cancer
Authors: Chocarro Luisa , Arasanz Hugo , Fernández-Rubio Leticia , Blanco Ester , Echaide Miriam , Bocanegra Ana , Teijeira Lucía , Garnica Maider , Morilla Idoia , Martínez-Aguillo Maite , Piñeiro-Hermida Sergio , Ramos Pablo , Lasarte Juan José , Vera Ruth , Kochan Grazyna , Escors David
Published: April 2022

Adoptive cell therapy with genetically modified T lymphocytes that express chimeric antigen receptors (CAR-T) is one of the most promising advanced therapies for the treatment of cancer, with unprecedented outcomes in hematological malignancies. However, the efficacy of CAR-T cells in solid tumors is still very unsatisfactory, because of the strong immunosuppressive tumor microenvironment that hinders immune responses. The development of next-generation personalized CAR-T cells against solid tumors is a clinical necessity. The identification of therapeutic targets for new CAR-T therapies to increase the efficacy, survival, persistence, and safety in solid tumors remains a critical frontier in cancer immunotherapy. Here, we summarize basic, translational, and clinical results of CAR-T cell immunotherapies in lung cancer, from their molecular engineering and mechanistic studies to preclinical and clinical development.
Effects of Mono-Vacancies of Oxygen and Manganese on the Properties of the MnO2/Graphene Heterostructure
Authors: Morinson-Negrete Juan David , Ortega-López César , Espitia-Rico Miguel J.
Published: April 2022

The effects of the monovacancies of oxygen (VO) and manganese (VMn) on the structural and electronic properties of the 1T–MnO2/graphene heterostructure are investigated, within the framework of density functional theory (DFT). We found that the values of the formation energy for the heterostructure without and with vacancies of VO and VMn were −20.99 meVÅ2 , −32.11meVÅ2, and −20.81 meVÅ2, respectively. The negative values of the formation energy indicate that the three heterostructures are energetically stable and that they could be grown in the experiment (exothermic processes). Additionally, it was found that the presence of monovacancies of VO and VMn in the heterostructure induce: (a) a slight decrease in the interlayer separation distance in the 1T–MnO2/graphene heterostructure of ~0.13% and ~1.41%, respectively, and (b) a contraction of the (Mn−O) bond length of the neighboring atoms of the VO and VMn monovacancies of ~2.34% and ~6.83%, respectively. Calculations of the Bader charge for the heterostructure without and with VO and VMn monovacancies show that these monovacancies induce significant changes in the charge of the first-neighbor atoms of the VO and VMn vacancies, generating chemically active sites (locales) that could favor the adsorption of external atoms and molecules. From the analysis of the density of state and the structure of the bands, we found that the graphene conserves the Dirac cone in the heterostructure with or without vacancies, while the 1T–MnO2 monolayer in the heterostructures without and with VO monovacancies exhibits half-metallic and magnetic behavior. These properties mainly come from the hybridization of the 3d–Mn and 2p–O states. In both cases, the heterostructure possesses a magnetic moment of 3.00 μβ/Mn. From this behavior, it can be inferred the heterostructures with and without VO monovacancies could be used in spintronics.
Ecodesign of the Aluminum Bronze Cutting Process
Authors: Dobrotă Dan , Oleksik Mihaela , Chicea Anca Lucia
Published: April 2022

The realization of products from materials with high properties generally involves very high energy consumption. Thus, in the research, it was considered to optimize the machining process by cutting of an aluminum bronze alloy, so as to obtain a reduction in energy consumption in correlation with the roughness of the machined surfaces. The research focused on the processing of a semi-finished product with a diameter of Ø = 20 mm made of aluminum bronze (C62300). In addition, in the research, the aim was to establish some correlations between the amount of power consumed and the quality of the surfaces processed by cutting. In this sense, the forces were measured in the 3 directions specific to the cutting process (Fc; Ff; Fp) for 3 tools construction variants and power consumed. The results showed that, if a certain constructive variant of the cutting tool is used in the processing, a reduction of the power consumed to cutting can be obtained by approximately 30% and a reduction of the roughness of the processed surface by approximately 90–100%. Furthermore, following the statistical processing of the results, it was shown that it would be advisable to use, especially in roughing processes, the cutting tool variant that offers the greatest reduction in roughness and cutting power.
Hybrid Lime–Pozzolan Geopolymer Systems: Microstructural, Mechanical and Durability Studies
Authors: Villca Ariel Rey , Soriano Lourdes , Borrachero María Victoria , Payá Jordi , Monzó José María , Tashima Mauro Mitsuuchi
Published: April 2022

This work studies the possibility of using geopolymer materials to enhance the mechanical and durability properties of hydrated lime–pozzolan mixtures, which gave rise to the so-called “hybrid systems”. Two different waste types were used as pozzolan in the lime–pozzolan system: rice husk ash (RHA) and spent fluid catalytic cracking (FCC). The geopolymer fabricated with FCC was activated with commercial reagents (NaOH and Na2SiO3), and also with alternative sources of silica to obtain a lower carbon footprint in these mixtures. The alternative silica sources were RHA and residual diatomaceous earth (RDE) from the beer industry. The geopolymer mixture substituted the lime–pozzolan mixture for 30% replacement in weight. The hybrid systems showed better mechanical strengths for the short and medium curing ages in relation to the lime–pozzolan mixtures. Thermogravimetric analyses were performed to characterise the types of products formed in these mixtures. In the durability studies, hybrid systems better performed in freeze–thaw cycles and obtained lower capillarity water absorption values.
Porous Carbon Material Derived from Steam-Exploded Poplar for Supercapacitor: Insights into Synergistic Effect of KOH and Urea on the Structure and Electrochemical Properties
Authors: Ding Dayong , Ma Lan , Li Xin , Liu Zhong , Hui Lanfeng , Zhang Fengshan , Zhao Yumeng
Published: April 2022

The electrochemical performance of supercapacitors using porous carbon as electrodes is strongly affected by the fabrication process of carbon material. KOH is commonly used as an activator combined with urea as a nitrogen dopant. However, the roles of KOH and urea in pore structure configuration and the electrochemical behavior of porous carbon electrodes are still ambiguous. Herein, the optimum porous carbon is obtained when KOH and urea are used simultaneously. KOH is used as a pore-forming substance, whereas urea is employed as a nitrogen source for the nitrogen doping of porous carbon, which increases its defect sites while reducing the graphitization degree. More importantly, urea also expands pores as a pore-enlarging agent, inducing interconnected porous structures. As a result, a hierarchical porous structure is formed and ascribed to the synergistic effect of KOH and urea, and the specific surface area reached 3282 m2 g−1 for sample PC800-4. The specific capacitance is 319 F g−1 at 0.5 A g−1 with excellent cycling stability over 2500 cycles. Furthermore, the symmetric supercapacitor reaches an excellent energy density of 11.6 W h kg−1 under 70.0 W kg−1 in a 6 M KOH electrolyte. Our work contributes to the rational designation of the porous carbon structure for supercapacitor applications.
Interpretable Machine Learning Algorithms to Predict the Axial Capacity of FRP-Reinforced Concrete Columns
Authors: Cakiroglu Celal , Islam Kamrul , Bekdaş Gebrail , Kim Sanghun , Geem Zong Woo
Published: April 2022

Fiber-reinforced polymer (FRP) rebars are increasingly being used as an alternative to steel rebars in reinforced concrete (RC) members due to their excellent corrosion resistance capability and enhanced mechanical properties. Extensive research works have been performed in the last two decades to develop predictive models, codes, and guidelines to estimate the axial load-carrying capacity of FRP-RC columns. This study utilizes the power of artificial intelligence and develops an alternative approach to predict the axial capacity of FRP-RC columns more accurately using data-driven machine learning (ML) algorithms. A database of 117 tests of axially loaded FRP-RC columns is collected from the literature. The geometric and material properties, column shape and slenderness ratio, reinforcement details, and FRP types are used as the input variables, while the load-carrying capacity is used as the output response to develop the ML models. Furthermore, the input-output relationship of the ML model is explained through feature importance analysis and the SHapely Additive exPlanations (SHAP) approach. Eight ML models, namely, Kernel Ridge Regression, Lasso Regression, Support Vector Machine, Gradient Boosting Machine, Adaptive Boosting, Random Forest, Categorical Gradient Boosting, and Extreme Gradient Boosting, are used in this study for capacity prediction, and their relative performances are compared to identify the best-performing ML model. Finally, predictive equations are proposed using the harmony search optimization and the model interpretations obtained through the SHAP algorithm.
Pressure Tuning of Superconductivity of LaPt4Ge12 and PrPt4Ge12 Single Crystals
Authors: Lombardi Gustavo A. , Mydeen Kamal , Gumeniuk Roman , Leithe-Jasper Andreas , Schnelle Walter , dos Reis Ricardo D. , Nicklas Michael
Published: April 2022

We carried out electrical resistivity and X-ray diffraction (XRD) studies on the filled skutterudite superconductors LaPt4Ge12 and PrPt4Ge12 under hydrostatic pressure. The superconducting transition temperature Tc is linearly suppressed upon increasing pressure, though the effect of pressure on Tc is rather weak. From the analysis of the XRD data, we obtain bulk moduli of B=106 GPa and B=83 GPa for LaPt4Ge12 and PrPt4Ge12, respectively. The knowledge of the bulk modulus allows us to compare the dependence of Tc on the unit-cell volume from our pressure study directly with that found in the substitution series La1−xPrxPt4Ge12. We find that application of hydrostatic pressure can be characterized mainly as a volume effect in LaPt4Ge12 and PrPt4Ge12, while substitution of Pr for La in La1−xPrxPt4Ge12 yields features going beyond a simple picture.
Preparation of Cotton–Zinc Composites by Magnetron Sputtering Metallization and Evaluation of their Antimicrobial Properties and Cytotoxicity
Authors: Kudzin Marcin Henryk , Giełdowska Małgorzata , Król Paulina , Sobańska Zuzanna
Published: April 2022

The aim of this investigation was to evaluate the biological properties of cotton–zinc composites. A coating of zinc (Zn) on a cotton fabric was successfully obtained by a DC magnetron sputtering system using a metallic Zn target (99.9%). The new composite was characterized using scanning electron microscopy/energy-dispersive X-ray spectroscopy (SEM/EDS), UV/Vis transmittance, and atomic absorption spectrometry with flame excitation (FAAS). The composite was tested for microbial activity against colonies of Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria and antifungal activity against Aspergillus niger and Chaetomium globosum fungal mold species as model microorganisms. Cytotoxicity screening of the tested modified material was carried out on BALB/3T3 clone mouse fibroblasts. The SEM/EDS and FAAS tests showed good uniformity of zinc content on a large surface of the composite. The conducted research showed the possibility of using the magnetron sputtering technique as a zero-waste method for producing antimicrobial textile composites.
Influence of Process Parameters on the Height and Performance of Magnesium Alloy AZ91D Internal Thread by Assisted Heating Extrusion
Authors: Liu Meng , Ji Zesheng , Bao Li , Li Xuemei
Published: April 2022

The quality of threaded connection is an important factor affecting the service life of equipment. Extruded thread has stronger mechanical properties than traditional cutting thread. The forming of magnesium alloy AZ91D internal thread by electromagnetic induction heating assisted extrusion is a new processing method. In this work, on the basis of this process, the height and performance of internal thread are selected as the evaluation index, and the response surface method is used to analyze the influence of the process parameters on the internal thread performance. The range of process parameters (auxiliary heating temperature, hole diameter, machine tool speed) is determined by slip line and empirical method, the test data are simulated, modeled and compared with the response surface analysis method, and the best mathematical model is selected to establish the regression model, the three-dimensional response surface curve of tooth height rate and maximum tensile force is obtained. Through simulation and prediction, it is found that the hole diameter and auxiliary heating temperature have significant influence on the tooth height rate and maximum tensile force of internal thread, and the order is that the hole diameter is larger than the auxiliary heating temperature than the machine tool speed. The research results show that the measured value of tooth height rate and maximum tensile force are close to the predicted value, and the errors are 1.8% and 2.7% respectively, and the model fits well. The better forming process parameters are as follows: auxiliary heating temperature to be 220 °C, hole diameter to be 11.35 mm, machine tool speed to be200 r/min. under this parameter, the tooth height rate and maximum tensile force to be 89.056% and 38.824 KN. At the same time, it is found that with the increase of thread height, the maximum tensile force of thread is also increasing, and the thread height affects the performance of thread. Finally, the optimal process parameters are obtained by response surface method, which improves the tensile properties of extruded internal threads.