bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluation of Adipose Tissue Zinc-Alpha 2-Glycoprotein Gene Expression and Its Relationship with Metabolic Status and Bariatric Surgery Outcomes in Patients with Class III Obesity
Authors: Martínez-Montoro José Ignacio , Ocaña-Wilhelmi Luis , Soler-Humanes Rocío , Motahari-Rad Hanieh , González-Jiménez Andrés , Rivas-Becerra José , Rodríguez-Muñoz Alba , Moreno-Ruiz Francisco J. , Tomé Mónica , Rodríguez-Capitán Jorge , García-Fuentes Eduardo , Tinahones Francisco J. , Garrido-Sánchez Lourdes , Murri Mora
Published: June 2022

Zinc-α2 glycoprotein (ZAG) is an adipokine involved in adipocyte metabolism with potential implications in the pathogenesis of metabolic disorders. Our aim was to evaluate the relationship between visceral (VAT) and subcutaneous adipose tissue (SAT) ZAG expression and metabolic parameters in patients with class III obesity, along with the impact of basal ZAG expression on short- and medium-term outcomes related to bariatric surgery. 41 patients with class III obesity who underwent bariatric surgery were included in this study. ZAG gene expression was quantified in SAT and VAT. Patients were classified into two groups according to SAT and VAT ZAG percentile. Anthropometric and biochemical variables were obtained before and 15 days, 45 days, and 1 year after surgery. The lower basal SAT ZAG expression percentile was associated with higher weight and waist circumference, while the lower basal VAT ZAG expression percentile was associated with higher weight, waist circumference, insulin, insulin resistance, and the presence of metabolic syndrome. Basal SAT ZAG expression was inversely related to weight loss at 45 days after surgery, whereas no associations were found between basal VAT ZAG expression and weight loss after surgery. Additionally, a negative association was observed between basal SAT and VAT ZAG expression and the decrease of gamma-glutamyl transferase after bariatric surgery. Therefore, lower SAT and VAT ZAG expression levels were associated with an adverse metabolic profile. However, this fact did not seem to confer worse bariatric surgery-related outcomes. Further research is needed to assess the clinical significance of the role of ZAG expression levels in the dynamics of hepatic enzymes after bariatric surgery.
Reconsidering the Polycystic Ovary Syndrome (PCOS)
Authors: Gleicher Norbert , Darmon Sarah , Patrizio Pasquale , Barad David H.
Published: June 2022

Though likely the most common clinical diagnosis in reproductive medicine, the Polycystic Ovary Syndrome (PCOS) is still only poorly understood. Based on previously published research, and here newly presented supportive evidence, we propose to replace the four current phenotypes of PCOS with only two entities—a hyperandrogenic phenotype (H-PCOS) including current phenotypes A, B, and C, and a hyper-/hypoandrogenic phenotype (HH-PCOS), representing the current phenotype D under the Rotterdam criteria. Reclassifying PCOS in this way likely establishes two distinct genomic entities, H-PCOS, primarily characterized by metabolic abnormalities (i.e., metabolic syndrome) and a hyperandrogenic with advancing age becoming a hypoandrogenic phenotype (HH-PCOS), in approximately 85% characterized by a hyperactive immune system mostly due to autoimmunity and inflammation. We furthermore suggest that because of hypoandrogenism usually developing after age 35, HH-PCOS at that age becomes relatively treatment resistant to in vitro fertilization (IVF) and offer in a case-controlled study evidence that androgen supplementation overcomes this resistance. In view of highly distinct clinical presentations of H-PCOS and HH-PCOS, polygenic risk scores should be able to differentiate between these 2 PCOS phenotypes. At least one clustering analysis in the literature is supportive of this concept.
Stem Cell-Based Therapy: A Promising Treatment for Diabetic Foot Ulcer
Authors: El Hage Racha , Knippschild Uwe , Arnold Tobias , Hinterseher Irene
Published: June 2022

Diabetic foot ulcer (DFU) is a severe complication of diabetes and a challenging medical condition. Conventional treatments for DFU have not been effective enough to reduce the amputation rates, which urges the need for additional treatment. Stem cell-based therapy for DFU has been investigated over the past years. Its therapeutic effect is through promoting angiogenesis, secreting paracrine factors, stimulating vascular differentiation, suppressing inflammation, improving collagen deposition, and immunomodulation. It is controversial which type and origin of stem cells, and which administration route would be the most optimal for therapy. We reviewed the different types and origins of stem cells and routes of administration used for the treatment of DFU in clinical and preclinical studies. Diabetes leads to the impairment of the stem cells in the diseased patients, which makes it less ideal to use autologous stem cells, and requires looking for a matching donor. Moreover, angioplasty could be complementary to stem cell therapy, and scaffolds have a positive impact on the healing process of DFU by stem cell-based therapy. In short, stem cell-based therapy is promising in the field of regenerative medicine, but more studies are still needed to determine the ideal type of stem cells required in therapy, their safety, proper dosing, and optimal administration route.
Heterogeneous Maturation of Arterio-Venous Fistulas and Loop-Shaped Venous Interposition Grafts: A Histological and 3D Flow Simulation Comparison
Authors: Szabo Balazs , Gasz Balazs , Fazekas Laszlo Adam , Varga Adam , Kiss-Papai Levente , Matolay Orsolya , Rezsabek Zsofia , Al-Smadi Mohammad W. , Nemeth Norbert
Published: June 2022

Vascular graft maturation is associated with blood flow characteristics, such as velocity, pressure, vorticity, and wall shear stress (WSS). Many studies examined these factors separately. We aimed to examine the remodeling of arterio-venous fistulas (AVFs) and loop-shaped venous interposition grafts, together with 3D flow simulation. Thirty male Wistar rats were randomly and equally divided into sham-operated, AVF, and loop-shaped venous graft (Loop) groups, using the femoral and superficial inferior epigastric vessels for anastomoses. Five weeks after surgery, the vessels were removed for histological evaluation, or plastic castings were made and scanned for 3D flow simulation. Remodeling of AVF and looped grafts was complete in 5 weeks. Histology showed heterogeneous morphology depending on the distribution of intraluminal pressure and WSS. In the Loop group, an asymmetrical WSS distribution coincided with the intima hyperplasia spots. The tunica media was enlarged only when both pressure and WSS were high. The 3D flow simulation correlated with the histological findings, identifying “hotspots” for intimal hyperplasia formation, suggesting a predictive value. These observations can be useful for microvascular research and for quality control in microsurgical training.
APTES-Modified Remote Self-Assembled DNA-Based Electrochemical Biosensor for Human Papillomavirus DNA Detection
Authors: Yang Yuxing , Qing Yang , Hao Xudong , Fang Chenxin , Ouyang Ping , Li Haiyu , Wang Zhencui , Liao Yazhen , Fang Haobin , Du Jie
Published: June 2022

High-risk human papillomavirus (HPV) infection is an important cause of cervical cancer formation; therefore, being able to detect high-risk HPV (e.g., HPV-16) is important for the early treatment and prevention of cervical cancer. In this study, a combination of a 3-aminopropyltriethoxysilane (APTES) modified gold electrode and a super sandwich structure was creatively developed, resulting in the development of a biosensor that is both sensitive and stable for the detection of HPV-16. The electrochemical biosensor possesses a lower detection limit compared with previous studies with an LOD of 5.475 × 10−16 mol/L and it possesses a wide linear range from 1.0 × 10−13 mol/L to 1.0 × 10−6 mol/L (R2 = 0.9923) for the target DNA. The experimental data show that the sensor has good stability, and there is no significant decrease in the current response value after 7 days in the low-temperature environment. In addition, the sensor proved to be a powerful clinical tool for disease diagnosis because it showed good interference resistance in complex human serum samples.
Microfluidics Facilitates the Development of Single-Cell RNA Sequencing
Authors: Pan Yating , Cao Wenjian , Mu Ying , Zhu Qiangyuan
Published: June 2022

Single-cell RNA sequencing (scRNA-seq) technology provides a powerful tool for understanding complex biosystems at the single-cell and single-molecule level. The past decade has been a golden period for the development of single-cell sequencing, with scRNA-seq undergoing a tremendous leap in sensitivity and throughput. The application of droplet- and microwell-based microfluidics in scRNA-seq has contributed greatly to improving sequencing throughput. This review introduces the history of development and important technical factors of scRNA-seq. We mainly focus on the role of microfluidics in facilitating the development of scRNA-seq technology. To end, we discuss the future directions for scRNA-seq.
Nanogap Electrode-Enabled Versatile Electrokinetic Manipulation of Nanometric Species in Fluids
Authors: Zhao Qiang , Wang Yunjiao , Sun Bangyong , Wang Deqiang , Li Gang
Published: June 2022

Noninvasive manipulation of nanoscopic species in liquids has attracted considerable attention due to its potential applications in diverse fields. Many sophisticated methodologies have been developed to control and study nanoscopic entities, but the low-power, cost-effective, and versatile manipulation of nanometer-sized objects in liquids remains challenging. Here, we present a dielectrophoretic (DEP) manipulation technique based on nanogap electrodes, with which the on-demand capturing, enriching, and sorting of nano-objects in microfluidic systems can be achieved. The dielectrophoretic control unit consists of a pair of swelling-induced nanogap electrodes crossing a microchannel, generating a steep electric field gradient and thus strong DEP force for the effective manipulation of nano-objects microfluidics. The trapping, enriching, and sorting of nanoparticles and DNAs were performed with this device to demonstrate its potential applications in micro/nanofluidics, which opens an alternative avenue for the non-invasive manipulation and characterization of nanoparticles such as DNA, proteins, and viruses.
New Microfluidic System for Electrochemical Impedance Spectroscopy Assessment of Cell Culture Performance: Design and Development of New Electrode Material
Authors: Chmayssem Ayman , Tanase Constantin Edi , Verplanck Nicolas , Gougis Maxime , Mourier Véronique , Zebda Abdelkader , Ghaemmaghami Amir M. , Mailley Pascal
Published: June 2022

Electrochemical impedance spectroscopy (EIS) is widely accepted as an effective and non-destructive method to assess cell health during cell-culture. However, there is a lack of compact devices compatible with microfluidic integration and microscopy that could provide the real-time and non-invasive monitoring of cell-cultures using EIS. In this paper, we reported the design and characterization of a modular EIS testing system based on a patented technology. This device was fabricated using easily processable methodologies including screen-printing of the impedance electrodes and molding or micromachining of the cell culture chamber with an easy assembly procedure. Accordingly, to obtain processable, biocompatible and sterilizable electrode materials that lower the impact of interfacial impedance on TEER (Transepithelial electrical resistance) measurements, and to enable concomitant microscopy observations, we optimized the formulation of the electrode inks and the design of the EIS electrodes, respectively. First, electrode materials were based on carbon biocompatible inks enriched with IrOx particles to obtain low interfacial impedance electrodes approaching the performances of classical non-biocompatible Ag/AgCl second-species electrodes. Secondly, we proposed three original electrode designs, which were compared to classical disk electrodes that were optically compatible with microscopy. We assessed the impact of the electrode design on the response of the impedance sensor using COMSOL Multiphysics. Finally, the performance of the impedance spectroscopy devices was assessed in vitro using human airway epithelial cell cultures.
Living Sample Viability Measurement Methods from Traditional Assays to Nanomotion
Authors: Al-madani Hamzah , Du Hui , Yao Junlie , Peng Hao , Yao Chenyang , Jiang Bo , Wu Aiguo , Yang Fang
Published: June 2022

Living sample viability measurement is an extremely common process in medical, pharmaceutical, and biological fields, especially drug pharmacology and toxicology detection. Nowadays, there are a number of chemical, optical, and mechanical methods that have been developed in response to the growing demand for simple, rapid, accurate, and reliable real-time living sample viability assessment. In parallel, the development trend of viability measurement methods (VMMs) has increasingly shifted from traditional assays towards the innovative atomic force microscope (AFM) oscillating sensor method (referred to as nanomotion), which takes advantage of the adhesion of living samples to an oscillating surface. Herein, we provide a comprehensive review of the common VMMs, laying emphasis on their benefits and drawbacks, as well as evaluating the potential utility of VMMs. In addition, we discuss the nanomotion technique, focusing on its applications, sample attachment protocols, and result display methods. Furthermore, the challenges and future perspectives on nanomotion are commented on, mainly emphasizing scientific restrictions and development orientations.
Advances of MXenes; Perspectives on Biomedical Research
Authors: Koyappayil Aneesh , Chavan Sachin Ganpat , Roh Yun-Gil , Lee Min-Ho
Published: June 2022

The last decade witnessed the emergence of a new family of 2D transition metal carbides and nitrides named MXenes, which quickly gained momentum due to their exceptional electrical, mechanical, optical, and tunable functionalities. These outstanding properties also rendered them attractive materials for biomedical and biosensing applications, including drug delivery systems, antimicrobial applications, tissue engineering, sensor probes, auxiliary agents for photothermal therapy and hyperthermia applications, etc. The hydrophilic nature of MXenes with rich surface functional groups is advantageous for biomedical applications over hydrophobic nanoparticles that may require complicated surface modifications. As an emerging 2D material with numerous phases and endless possible combinations with other 2D materials, 1D materials, nanoparticles, macromolecules, polymers, etc., MXenes opened a vast terra incognita for diverse biomedical applications. Recently, MXene research picked up the pace and resulted in a flood of literature reports with significant advancements in the biomedical field. In this context, this review will discuss the recent advancements, design principles, and working mechanisms of some interesting MXene-based biomedical applications. It also includes major progress, as well as key challenges of various types of MXenes and functional MXenes in conjugation with drug molecules, metallic nanoparticles, polymeric substrates, and other macromolecules. Finally, the future possibilities and challenges of this magnificent material are discussed in detail.
Effects of a 10-Week Physical Activity Intervention on Asylum Seekers’ Physiological Health
Authors: Guerra Matheus , Garcia Danilo , Kazemitabar Maryam , Lindskär Erik , Schütz Erica , Berglind Daniel
Published: June 2022

Introduction: The rise in armed conflicts has contributed to an increase in the number of asylum seekers. Prolonged asylum processes may negatively affect asylum seekers’ health and lead to inactivity. Studies show that physical activity interventions are associated with improvements in health outcomes. However, there are a limited number of studies investigating the associations of physical activity on asylum seekers’ health. Methods: Participants (263 males and 204 females), mostly from Syria, were assessed before and after a 10-week intervention for VO2 max, body mass index (BMI), skeletal muscle mass (SMM), body fat, and visceral fat. Linear mixed models were used to test differences within groups, and a linear regression model analysis was performed to test whether physiological variables predicted adherence. Results: Participants’ VO2 max increased: males by 2.96 mL/min/kg and females 2.57 mL/min/kg. Increased SMM percentages were seen in both genders: females by 0.38% and males 0.23%. Visceral fat area decreased: males by 0.73 cm2 and females 5.44 cm2. Conclusions: Participants showed significant increases in VO2 max and SMM and decreased visceral fat. This study provides an insight into asylum seekers’ health and serves as a starting point to new interventions in which physical activity is used as a tool to promote and improve vulnerable populations’ health.
Corticospinal Excitability and Inhibition Are Not Different between Concussed Males and Females
Authors: Pauhl Alexandra , Yasen Alia , Christie Anita
Published: June 2022

It has been consistently demonstrated that females report greater numbers of concussions in sex-comparable sports and take longer to recover from concussive symptoms than males. However, it is unknown if the neurophysiological consequences of concussion may contribute to these sex differences in concussion symptoms and recovery. The purpose of this study was to examine potential sex-related differences in neurophysiology in healthy and concussed individuals. Twenty-one (nine F) concussed individuals (20.9 ± 4.1 years; CONC) and twenty-one age-, sex-, height-, weight-, and activity-matched controls (21.2 ± 4.2 years; CONT) participated in the study. The CONC group reported to the lab within 72 h, 1-week, and 2-weeks post-injury and the CONT group followed a similar measurement schedule. Using transcranial magnetic stimulation, motor evoked potential (MEP) amplitude and cortical silent period (CSP) duration were measured from the first dorsal interosseous muscle to assess corticospinal excitability and inhibition, respectively. There were no significant differences across time (p ≥ 0.13) or between the CONC and CONT group in MEP amplitude (p = 0.72) or CSP duration (p = 0.54). Overall, males (119.08 ± 29.91 ms) had significantly longer CSP durations compared with females (101.24 ± 33.43 ms), indicating greater corticospinal inhibition in males, regardless of injury status (p = 0.04). An important and novel finding of this study was the lack of differences in these neurophysiological measures between males and females following concussion. To our knowledge, this is the first study to document greater corticospinal inhibition in males compared with females.
Gene Expression Changes of Murine Cortex Homeostasis in Response to Sleep Deprivation Hint Dysregulated Aging-like Transcriptional Responses
Authors: Giannos Panagiotis , Prokopidis Konstantinos , Forbes Scott C. , Celoch Kamil , Candow Darren G. , Tartar Jaime L.
Published: June 2022

Sleep deprivation leads to the deterioration in the physiological functioning of the brain, cognitive decline, and many neurodegenerative diseases, all of which progress with advancing age. Sleep insufficiency and impairments in cognitive function are characterized by progressive neuronal losses in the cerebral cortex. In this study, we analyze gene expression profiles following sleep-deprived murine models and circadian matched controls to identify genes that might underlie cortical homeostasis in response to sleep deprivation. Screening of the literature resulted in three murine (Mus musculus) gene expression datasets (GSE6514, GSE78215, and GSE33491) that included cortical tissue biopsies from mice that are sleep deprived for 6 h (n = 15) and from circadian controls that are left undisturbed (n = 15). Cortical differentially expressed genes are used to construct a network of encoded proteins that are ranked based on their interactome according to 11 topological algorithms. The analysis revealed three genes—NFKBIA, EZR, and SGK1—which exhibited the highest multi-algorithmic topological significance. These genes are strong markers of increased brain inflammation, cytoskeletal aberrations, and glucocorticoid resistance, changes that imply aging-like transcriptional responses during sleep deprivation in the murine cortex. Their potential role as candidate markers of local homeostatic response to sleep loss in the murine cortex warrants further experimental validation.
Predicting Hypnotic Use among Insomnia Patients with the Theory of Planned Behavior and Craving
Authors: Yang Chien-Ming , Lai Yu-Shuan , Huang Yun-Hsin , Huang Ya-Chuan , Lee Hsin-Chien
Published: June 2022

While long-term hypnotic use is very common in clinical practice, the associated factors have been understudied. This study aims to explore the cognitive factors that might influence the long-term use of hypnotics based on the theory of planned behavior (TPB), and examines the moderating effect of craving between cognitive intention and actual hypnotic-use behavior at follow-up. A total of 139 insomnia patients completed a self-constructed TPB questionnaire to measure their attitude, subjective norm, perceived behavioral control, and behavioral intention of hypnotic use, as well as the Hypnotic-Use Urge Scale (HUS) to measure their craving for hypnotics. They were then contacted through phone approximately three months later to assess their hypnotic use. Hierarchical regression showed that perceived behavioral control was the most significant determinant for behavioral intention of hypnotic use. Behavioral intention, in turn, can predict the frequency of hypnotic use after three months. However, this association was moderated by hypnotic craving. The association was lower among the participants with higher cravings for hypnotic use. The findings suggest that the patients’ beliefs about their control over sleep and daily life situations, and their craving for hypnotics should be taken into consideration in the management of hypnotic use.
The Relationships between Cognitive Styles and Creativity: The Role of Field Dependence-Independence on Visual Creative Production
Authors: Giancola Marco , Palmiero Massimiliano , Piccardi Laura , D’Amico Simonetta
Published: June 2022

Previous studies explored the relationships between field dependent-independent cognitive style (FDI) and creativity, providing misleading and unclear results. The present research explored this problematic interplay through the lens of the Geneplore model, employing a product-oriented task: the Visual Creative Synthesis Task (VCST). The latter requires creating objects belonging to pre-established categories, starting from triads of visual components and consists of two steps: the preinventive phase and the inventive phase. Following the Amabile’s consensual assessment technique, three independent judges evaluated preinventive structures in terms of originality and synthesis whereas inventions were evaluated in terms of originality and appropriateness. The Embedded Figure Test (EFT) was employed in order to measure the individual’s predisposition toward the field dependence or the field independence. Sixty undergraduate college students (31 females) took part in the experiment. Results revealed that field independent individuals outperformed field dependent ones in each of the four VCST scores, showing higher levels of creativity. Results were discussed in light of the better predisposition of field independent individuals in mental imagery, mental manipulation of abstract objects, as well as in using their knowledge during complex tasks that require creativity. Future research directions were also discussed.
Experimental and Numerical Investigation of Plastic–Concrete Waterproof Walls of an Underground Granary Subject to Combined Bending Moment and Water Pressure
Authors: Zhang Hao , Han Kaiyi , Yang Jinping , Chen Lei
Published: June 2022

To investigate the mechanical properties of plastic–concrete silo walls in practice, the mechanical properties and failure mechanism under the combined bending moment and water pressure were analyzed through the uniform loading test, water pressure test, and numerical analysis. The influence of the connecting plate spacing, radius, and the waterproof plate thickness on the water pressure-bearing capacity were analyzed. The test results show that the chemical adhesive force exists between the waterproof plate and concrete and can resist 20 kPa. The displacement and strain of the waterproof plate increases significantly with the increment in water pressure. When the water pressure reached 85 kPa, the specimen was damaged due to shear failure. The established numerical model was validated by the test results. The numerical analysis results show that the specimen failure mainly depends on the bolt strength when the thickness of the waterproof plate is greater than 14 mm or the radius of the connecting plate is greater than 60 mm. The relation between the design parameters and the water pressure-bearing capacity was proposed. Compared with the waterproof plate thickness, the connecting plate spacing and radius have greater influence on the water pressure-bearing capacity.
Mechanical Properties of Prefabricated Cold-Formed Steel Stud Wall Panels Sheathed with Fireproof Phenolic Boards under Out-of-Plane Loading
Authors: Zhao Gengqi , Chen Wanqiong , Zhao Dapeng , Li Ke
Published: June 2022

In this paper, new prefabricated cold-formed light-gauge steel stud wall panels sheathed with fireproof phenolic boards, which are fabricated by connecting the steel studs and the boards by using structural silicone sealant, was proposed. The proposed prefabricated wall has a good fireproof performance and can be manufactured rapidly in a factory. Full-scale tests on the mechanical properties of the prefabricated wall system, consisting of the prefabricated wall and the connection between the wall and the surrounding steel structure under out-of-plane loading, were performed. A total of six specimens were tested considering the effects of the arrangement of the cold-formed light-gauge steel studs, the shape and thickness of the steel connector for jointing the prefabricated wall panel and the surrounding steel structure, and the number of self-tapping screws connecting the surrounding structure. The results show that the out-of-plane stiffness of the prefabricated wall system in the elastic stress state under out-of-plane loading can be increased by increasing the number of self-tapping screws, increasing the thickness of the steel connector, or adopting the symmetrical arrangement of the light-gauge steel studs. The out-of-plane stiffness of the prefabricated wall system and the stiffness contribution of a single special-shaped steel connector can both be increased by increasing the number of special-shaped steel connectors. Furthermore, the special-shaped steel connector is more beneficial to a greater out-of-plane stiffness than the L-shaped steel connector. In addition, the theoretical calculation methods for deflection of the proposed prefabricated wall and flexural stress of the CFS C-channel stud considering the fireproof phenolic board sheathing effect under elastic state were proposed. The predicted results using the proposed method are compared with test results and the predicted results by using other methods. It was found that the predicted results by using the proposed method agreed better with the test results compared with the predicted results using the transformed-section method or the reduced stiffness method, which demonstrates the acceptability and accuracy of the proposed mothed for calculating deflection of the proposed prefabricated wall and flexural stress of the CFS C-channel stud.
Structural Materials, Ventilation Design and Architectural Art of Traditional Buildings in Guangdong, China
Authors: Song Yingqiu , Liao Chenyue
Published: June 2022

Due to its special geographical conditions, Guangdong has absorbed the essence of the Central Plains Han culture and characteristics of foreign cultures, resulting in architecture in which traditional buildings exhibit unique regional cultural connotations. The architectural design is adapted to the needs of nature and ecology and the arts and humanities. This paper investigates the technical process of using local materials to obtain structural materials. Traditional Guangdong buildings use patios, cold alleys and doors as well as windows in a comprehensive manner to create an energy-saving and environmentally friendly ventilation design, which was analyzed here. In addition, we study the floor plans and artistic characteristics of traditional buildings in different regions of Guangdong. Traditional Guangdong buildings realize the harmonious coexistence of humans and nature, as well as embody the concept of sustainable development. Traditional buildings integrate benefits and efficiency while condensing the surpassing wisdom and rich experience of craftsmen from ancient times to the present. Thus, it is worthwhile to research the connotation of traditional buildings to develop modern architectures.
Developing Heat Mitigation Strategies in the Urban Environment of Sydney, Australia
Authors: Heshmat Mohajer Hamed Reza Heshmat , Ding Lan , Santamouris Mattheos
Published: June 2022

Heat island effects raise the ambient air temperature in metropolitan areas by 4–5 degrees Celsius and can reach 10 degrees Celsius at their maximum. This phenomenon magnifies cities’ energy difficulties while reducing comfort. Mitigation strategies have been developed and recommended to deal with the issue. Methods to increase albedo and the utilisation of vegetation appear to be the most promising, with a reasonably high heat island reduction capacity. This paper examines the heat mitigation techniques and their effectiveness under Sydney’s climate conditions and compares strategies. We implement two perspectives, namely urban greening (green roofs, green pavements) and albedo (street, roof), and characterise urban surface structures, and Envi-met software is employed for our simulation method. Mitigation strategies show a cooling potential of 4.1 °C in temperature along this precinct during the heatwave period. Scenarios that increase high-albedo material on the road, pavements and rooftops and full mitigation show the maximum cooling potential. The mitigation strategies have higher predicted cooling potential on the peak ambient temperature, up to 1.18 °C, while having no or little impact on minimum ambient temperature. The outdoor thermal comfort based on PMV indices varies between a minimum of −0.33 in scenario seven in large layout areas to 3. However, the mitigation scenario presents more acceptable outdoor thermal comfort, but large layouts are predicted to have a hot condition.
Inhibition of RNA Binding in SND1 Increases the Levels of miR-1-3p and Sensitizes Cancer Cells to Navitoclax
Authors: Lehmusvaara Saara , Haikarainen Teemu , Saarikettu Juha , Martinez Nieto Guillermo , Silvennoinen Olli
Published: June 2022

SND1 is an RNA-binding protein overexpressed in large variety of cancers. SND1 has been proposed to enhance stress tolerance in cancer cells, but the molecular mechanisms are still poorly understood. We analyzed the expression of 372 miRNAs in the colon carcinoma cell line and show that SND1 silencing increases the expression levels of several tumor suppressor miRNAs. Furthermore, SND1 knockdown showed synergetic effects with cancer drugs through MEK-ERK and Bcl-2 family-related apoptotic pathways. To explore whether the SND1-mediated RNA binding/degradation is responsible for the observed effect, we developed a screening assay to identify small molecules that inhibit the RNA-binding function of SND1. The screen identified P2X purinoreceptor antagonists as the most potent inhibitors. Validation confirmed that the best hit, suramin, inhibits the RNA binding ability of SND1. The binding characteristics and mode of suramin to SND1 were characterized biophysically and by molecular docking that identified positively charged binding cavities in Staphylococcus nuclease domains. Importantly, suramin-mediated inhibition of RNA binding increased the expression of miR-1-3p, and enhanced sensitivity of cancer cells to Bcl-2 inhibitor navitoclax treatment. Taken together, we demonstrate as proof-of-concept a mechanism and an inhibitor compound for SND1 regulation of the survival of cancer cells through tumor suppressor miRNAs.
Current and Future Immunotherapy-Based Treatments for Oesophageal Cancers
Authors: To Natalie , Evans Richard P. T. , Pearce Hayden , Kamarajah Sivesh K. , Moss Paul , Griffiths Ewen A.
Published: June 2022

Oesophageal cancer is a disease that causes significant morbidity and mortality worldwide, and the prognosis of this condition has hardly improved in the past few years. Standard treatment includes a combination of chemotherapy, radiotherapy and surgery; however, only a proportion of patients go on to treatment intended to cure the disease due to the late presentation of this disease. New treatment options are of utmost importance, and immunotherapy is a new option that has the potential to transform the landscape of this disease. This treatment is developed to act on the changes within the immune system caused by cancer, including checkpoint inhibitors, which have recently shown great promise in the treatment of this disease and have recently been included in the adjuvant treatment of oesophageal cancer in many countries worldwide. This review will outline the mechanisms by which cancer evades the immune system in those diagnosed with oesophageal cancer and will summarize current and ongoing trials that focus on the use of our own immune system to combat disease.
Mesothelioma Mouse Models with Mixed Genomic States of Chromosome and Microsatellite Instability
Authors: Song Yurong , Baxter Shaneen S. , Dai Lisheng , Sanders Chelsea , Burkett Sandra , Baugher Ryan N. , Mellott Stephanie D. , Young Todd B. , Lawhorn Heidi E. , Difilippantonio Simone , Karim Baktiar , Kadariya Yuwaraj , Pinto Ligia A. , Testa Joseph R. , Shoemaker Robert H.
Published: June 2022

Malignant mesothelioma (MMe) is a rare malignancy originating from the linings of the pleural, peritoneal and pericardial cavities. The best-defined risk factor is exposure to carcinogenic mineral fibers (e.g., asbestos). Genomic studies have revealed that the most frequent genetic lesions in human MMe are mutations in tumor suppressor genes. Several genetically engineered mouse models have been generated by introducing the same genetic lesions found in human MMe. However, most of these models require specialized breeding facilities and long-term exposure of mice to asbestos for MMe development. Thus, an alternative model with high tumor penetrance without asbestos is urgently needed. We characterized an orthotopic model using MMe cells derived from Cdkn2a+/−;Nf2+/− mice chronically injected with asbestos. These MMe cells were tumorigenic upon intraperitoneal injection. Moreover, MMe cells showed mixed chromosome and microsatellite instability, supporting the notion that genomic instability is relevant in MMe pathogenesis. In addition, microsatellite markers were detectable in the plasma of tumor-bearing mice, indicating a potential use for early cancer detection and monitoring the effects of interventions. This orthotopic model with rapid development of MMe without asbestos exposure represents genomic instability and specific molecular targets for therapeutic or preventive interventions to enable preclinical proof of concept for the intervention in an immunocompetent setting.
Amygdalin Exerts Antitumor Activity in Taxane-Resistant Prostate Cancer Cells
Authors: Tsaur Igor , Thomas Anita , Monecke Michelle , Zugelder Marion , Rutz Jochen , Grein Timothy , Maxeiner Sebastian , Xie Hui , Chun Felix K.-H. , Rothweiler Florian , Cinatl Jindrich , Michaelis Martin , Haferkamp Axel , Blaheta Roman A.
Published: June 2022

Despite recent advances in the treatment of metastatic prostate cancer (PCa), resistance development after taxane treatments is inevitable, necessitating effective options to combat drug resistance. Previous studies indicated antitumoral properties of the natural compound amygdalin. However, whether amygdalin acts on drug-resistant tumor cells remains questionable. An in vitro study was performed to investigate the influence of amygdalin (10 mg/mL) on the growth of a panel of therapy-naïve and docetaxel- or cabazitaxel-resistant PCa cell lines (PC3, DU145, and LNCaP cells). Tumor growth, proliferation, clonal growth, and cell cycle progression were investigated. The cell cycle regulating proteins (phospho)cdk1, (phospho)cdk2, cyclin A, cyclin B, p21, and p27 and the mammalian target of rapamycin (mTOR) pathway proteins (phospho)Akt, (phospho)Raptor, and (phospho)Rictor as well as integrin β1 and the cytoskeletal proteins vimentin, ezrin, talin, and cytokeratin 8/18 were assessed. Furthermore, chemotactic activity and adhesion to extracellular matrix components were analyzed. Amygdalin dose-dependently inhibited tumor growth and reduced tumor clones in all (parental and resistant) PCa cell lines, accompanied by a G0/G1 phase accumulation. Cell cycle regulating proteins were significantly altered by amygdalin. A moderate influence of amygdalin on tumor cell adhesion and chemotaxis was observed as well, paralleled by modifications of cytoskeletal proteins and the integrin β1 expression level. Amygdalin may, therefore, block tumor growth and disseminative characteristics of taxane-resistant PCa cells. Further studies are warranted to determine amygdalin’s value as an antitumor drug.
Single B Cell Gene Co-Expression Networks Implicated in Prognosis, Proliferation, and Therapeutic Responses in Non-Small Cell Lung Cancer Bulk Tumors
Authors: Ye Qing , Guo Nancy Lan
Published: June 2022

In NSCLC, there is a pressing need for immunotherapy predictive biomarkers. The processes underlying B-cell dysfunction, as well as their prognostic importance in NSCLC, are unknown. Tumor-specific B-cell gene co-expression networks were constructed by comparing the Boolean implication modeling of single-cell RNA sequencing of NSCLC tumor B cells and normal B cells. Proliferation genes were selected from the networks using in vitro CRISPR-Cas9/RNA interfering (RNAi) screening data in more than 92 human NSCLC epithelial cell lines. The prognostic and predictive evaluation was performed using public NSCLC transcriptome and proteome profiles. A B cell proliferation and prognostic gene co-expression network was present only in normal lung B cells and missing in NSCLC tumor B cells. A nine-gene signature was identified from this B cell network that provided accurate prognostic stratification using bulk NSCLC tumor transcriptome (n = 1313) and proteome profiles (n = 103). Multiple genes (HLA-DRA, HLA-DRB1, OAS1, and CD74) differentially expressed in NSCLC B cells, peripheral blood lymphocytes, and tumor T cells had concordant prognostic indications at the mRNA and protein expression levels. The selected genes were associated with drug sensitivity/resistance to 10 commonly used NSCLC therapeutic regimens. Lestaurtinib was discovered as a potential repositioning drug for treating NSCLC.
Photodegradation under UV Light Irradiation of Various Types and Systems of Organic Pollutants in the Presence of a Performant BiPO4 Photocatalyst
Authors: Bouddouch Abdessalam , Akhsassi Brahim , Amaterz Elhassan , Bakiz Bahcine , Taoufyq Aziz , Villain Sylvie , Guinneton Frédéric , El Aamrani Abdelaziz , Gavarri Jean-Raymond , Benlhachemi Abdeljalil
Published: June 2022

In this study, we prepared spheroid microstructures of monoclinic bismuth phosphate BiPO4 by a facile solid-state reaction at 500 °C. The crystal structure was refined using the Rietveld method, where the crystal cell was resolved using a monoclinic system (parameters a, b, c, β) with space group P21/n. SEM images showed that the solid catalyst presented homogeneous morphologies. These BiPO4 microparticles (BiP-500) have been used as photocatalysts to photodegrade, under UV light irradiation, three cationic dyes (Rhodamine B, RhB; Methylene Blue, MB; and Toluidine Blue, TB), three anionic dyes (Congo Red, CR; Orange G, OG; and Methyl Orange, MO) and mixtures of RhB-MB, RhB-OG and MO-OG organic dyes. The photodegradation efficiency of these BiP-500 microparticles is found to be optimal in the case of RhB solutions, RhB-MB and RhB-OG binary mixtures. The BiP-500 catalyst shows a high selectivity for the conversion of the mixture of dyes into CO2 and H2O. Total organic carbon analysis of an anionic dye and a cationic dye (RhB, OG and RhB-MB) confirms the mineralization of the pollutants in the presence of BiP-500 particles. The photocatalytic efficiency of our BiP-500 photocatalyst has been confirmed, with a view to facilitate applications in the field of the depollution of wastewater in the agricultural environment by the degradation of parathion-methyl (PM) as a pollutant.
C058 and Other Functional Microorganisms Promote the Synthesis of Extracellular Polymer Substances in Mycelium Biofloc
Authors: Li Yiyong , Luo Wanyi , Liu Wen , Yang Yongcong , Lei Zexiang , Tao Xueqin , Wang Baoe
Published: June 2022

The mycelium biofloc bioaugmented by Cordyceps strain C058 effectively purifies water, which may be related to the synthesis of extracellular polymer substances. To verify this conjecture, we analyzed the changes in extracellular polymer substances content in the mycelium biofloc under various hydraulic retention times (36 h, 18 h, and 11 h). The microstructure and microflora composition were analyzed using a scanning electron microscope and high-throughput sequencing. The ordinary biofloc without bioaugmentation was taken as a control. The results showed that under the above hydraulic retention time, the extracellular polymer substances contents of the mycelium biofloc were 51.20, 55.89, and 33.84 mg/g, respectively, higher than that of the ordinary biofloc (14.58, 15.72, and 18.19 mg/g). The protein content or the polysaccharide content also followed the same trend. Meanwhile, the sedimentation performance of the mycelium biofloc was better than that of the ordinary biofloc, attributed to the content of the extracellular polymer substances. It is worth noting that C058 is the main biofloc content, which promotes the synthesis of extracellular polymer substances in the mycelium biofloc. Other functional microorganisms in the mycelium biofloc were Janthinobacterium, Phormidium, Leptolyngbya, Hymenobacter, and Spirotrichea, which also promote the synthesis of extracellular polymer substances.
Effect of Different Iron Phases of Fe/SiO2 Catalyst in CO2 Hydrogenation under Mild Conditions
Authors: Sirikulbodee Paphatsara , Phongaksorn Monrudee , Sornchamni Thana , Ratana Tanakorn , Tungkamani Sabaithip
Published: June 2022

The effect of different active phases of Fe/SiO2 catalyst on the physio-chemical properties and the catalytic performance in CO2 hydrogenation under mild conditions (at 220 °C under an ambient pressure) was comprehensively studied in this work. The Fe/SiO2 catalyst was prepared by an incipient wetness impregnation method. Hematite (Fe2O3) in the calcined Fe/SiO2 catalyst was activated by hydrogen, carbon monoxide, and hydrogen followed by carbon monoxide, to form a metallic iron (Fe/SiO2-h), an iron carbide (Fe/SiO2-c), and a combination of a metallic iron and an iron carbide (Fe/SiO2-hc), respectively. All activated catalysts were characterized by XRD, Raman spectroscopy, N2 adsorption–desorption, H2-TPR, CO-TPR, H2-TPD, CO2-TPD, CO-TPD, NH3-TPD, and tested in a CO2 hydrogenation reaction. The different phases of the Fe/SiO2 catalyst are formed by different activation procedures and different reducing agents (H2 and CO). Among three different activated catalysts, the Fe/SiO2-c provides the highest CO2 hydrogenation performance in terms of maximum CO2 conversion, as well as the greatest selectivity toward long-chain hydrocarbon products, with the highest chain growth probability of 0.7. This is owing to a better CO2 and CO adsorption ability and a greater acidity on the carbide form of the Fe/SiO2-c surface, which are essential properties of catalysts for polymerization in FTs.
Catalytic Effect of CO2 and H2O Molecules on •CH3 + 3O2 Reaction
Authors: Ali Mohamad Akbar , Dash Manas Ranjan , Al Maieli Latifah Mohammed
Published: June 2022

The methyl (•CH3) + 3O2 radical is an important reaction in both atmospheric and combustion processes. We investigated potential energy surfaces for the effect of CO2 and H2O molecules on a •CH3+ O2 system. The mechanism for three reaction systems, i.e., for •CH3 + 3O2, •CH3 + 3O2 (+CO2) and •CH3 + 3O2 (+H2O), were explored using ab initio/DFT methods [CCSD(T)//M062X/6-311++G(3df,3pd)] in combination with a Rice−Ramsperger−Kassel−Marcus (RRKM)/master-equation (ME) simulation between a temperature range of 500 to 1500 K and a pressure range of 0.0001 to 10 atm. When a CO2 and H2O molecule is introduced in a •CH3 + 3O2 reaction, the reactive complexes, intermediates, transition states and post complexes become thermodynamically more favorable. The calculated rate constant for the •CH3 + 3O2 (3 × 10−15 cm3 molecule−1 s−1 at 1000 K) is in good agreement with the previously reported experimentally measured values (~1 × 10−15 cm3 molecule−1 s−1 at 1000 K). The rate constant for the effect of CO2 (3 × 10−16 cm3 molecule−1 s−1 at 1000 K) and H2O (2 × 10−17 cm3 molecule−1 s−1 at 1000 K) is at least one–two-order magnitude smaller than the free reaction (3 × 10−15 cm3 molecule−1 s−1 at 1000 K). The effect of CO2 and H2O on •CH3 + 3O2 shows non-RRKM behavior, however, the effect on •CH3 + 3O2 shows RRKM behavior. Our results also demonstrate that a single CO2 and H2O molecule has the potential to accelerate a gas-phase reaction at temperature higher than >1300 K and slow the reaction at a lower temperature. The result is unique and observed for the first time.
Explaining Redundancy in CDK-Mediated Control of the Cell Cycle: Unifying the Continuum and Quantitative Models
Authors: Fisher Daniel , Krasinska Liliana
Published: June 2022

In eukaryotes, cyclin-dependent kinases (CDKs) are required for the onset of DNA replication and mitosis, and distinct CDK–cyclin complexes are activated sequentially throughout the cell cycle. It is widely thought that specific complexes are required to traverse a point of commitment to the cell cycle in G1, and to promote S-phase and mitosis, respectively. Thus, according to a popular model that has dominated the field for decades, the inherent specificity of distinct CDK–cyclin complexes for different substrates at each phase of the cell cycle generates the correct order and timing of events. However, the results from the knockouts of genes encoding cyclins and CDKs do not support this model. An alternative “quantitative” model, validated by much recent work, suggests that it is the overall level of CDK activity (with the opposing input of phosphatases) that determines the timing and order of S-phase and mitosis. We take this model further by suggesting that the subdivision of the cell cycle into discrete phases (G0, G1, S, G2, and M) is outdated and problematic. Instead, we revive the “continuum” model of the cell cycle and propose that a combination with the quantitative model better defines a conceptual framework for understanding cell cycle control.