bluuebunny/crossref_metadata_2025 huggingface dataset:
Applying the UDA-ODA discrimination technique to a herpetological association: the case of the Middle Palaeolithic site of El Salt (Alcoi, Spain)
Authors: Marquina-Blasco Rafael , Fagoaga Ana , Crespo Vicente D. , Bailon Salvador , Mallol Carolina , Hernández Cristo M. , Galván Bertila , Blain Hugues-Alexandre , Ruiz-Sánchez Francisco Javier
Published: July 2022

El Salt (Middle Palaeolithic; Alcoi, Spain) is a key site for understanding the disappearance of Neanderthals in the eastern Iberian Peninsula, a process that is observed along its stratigraphic sequence. To improve our understanding of the palaeoclimatic context in which this process took place, we applied the UDA-ODA discrimination technique to the fossil herpetological assemblages from Stratigraphic Units (SU) Xb (52.3 ± 4.6 ka) and V (45.2 ± 3.4/44.7 ± 3.4 ka). This method is based in the application of an ecological criterion, the maximum altitude of each species on each Iberian range, to discern and remove those areas that do not meet their ecological requirements (UDA) from those that do (ODA), which are included in its distribution from Atlases from which the common distribution species is going to be extracted. Results indicate the existence of an alternation between “cold and wet” moments with “warm and dry” ones, all of them in a more humid context than nowadays. Climate was slightly warmer than nowadays in SU Xb, whereas was cooler in SU V. Regarding MAP, our results show less variation between SUs than it has been provided by previous studies. These results do not fully agree with those provided by small mammals from the same SUs, which indicate an increase of the temperatures whereas rainfall experienced the contrary. These differences could be related with the wider distribution of the Iberian herpetofauna, and some differences in the applied ecological criterion. Furthermore, herpetofaunal palaeoclimatic reconstruction shows that the abandonment of the site by Neanderthals seems to have occurred during an arid phase.
Recycling of Mediterranean Fruit Fly Rearing Waste by Black Soldier Fly, Hermetia illucens
Authors: Sayed Waheed A. A. , Alm-Eldin Magdy M. S. , Hassan Reda S. , Sileem Thanaa M. , Rumpold Birgit A.
Published: July 2022

Purpose

Objective of this study is the valorisation of mass rearing waste of fruit flies from sterile insect technique facilities by black soldier flies into high quality and quantity products as a sustainable utilisation concept.

Methods

Different ratios of medfly rearing waste in hen feed were used for rearing black solder fly (BSF) larvae. The growth rate and insect survival were determined. Moreover, the mass gains were determined and bioconversion rates were assessed. Furthermore, the nutritional compositions of BSF pupae were analysed to evaluate the product quality.

Results

The omnivorous BSF larvae were highly efficient at converting digested waste into body mass as compared to the control treatment (hen feed). High weight of pre-pupae was recorded for all experimental diets. The efficacy of larvae to reach pupae ranged from 92.5 to 98.5%, while the efficacy of pupae to reach adults ranged from 81.7 to 89.0%. All experimental diets exhibited high rates of prepupal weight, metabolism and efficiency of conversion of digested food. A nutritional analysis revealed that the protein and fat contents were high, while the bioaccumulation of heavy metals was low,

Conclusion

Medfly rearing waste is a potential feed ingredient for the production of BSF pre-pupae and could be applied to valorise this rearing waste into high-value feed.

Graphical Abstract
Microplastic pollution in sediments in the urban section of the Qara Su River, Iran
Authors: Ghanbari Nouraddin , Fataei Ebrahim , Naji Abolfazl , Imani Ali Akbar , Nasehi Fatemeh
Published: July 2022

Microplastic pollution is an emerging threat to marine environments with potential environmental, social, economic, and health consequences and has become a major global environmental issue. The objective of the present study was to evaluate microplastic contamination in the ecosystem of the Qara Su River in Ardabil, Iran. Fifteen sampling sites along the Qara Su River in the Ardabil urban area were selected to collect sediment samples. The abundance and morphological characteristics of microplastics were determined by counting using a digital stereomicroscope. Micro-Fourier transform-infrared spectroscopy (μ-FTIR) analysis was used to identify polymer type of the microplastics. In this study, the frequency and distribution of microplastics (< 5 mm) in the sediment of Qara Su River were investigated as one of the sources containing microplastics. Sediment samples were collected from five different sites (Karkarq, Sar band, Anzab Sulfa, Dolat abad, and Samian) from September to March 2020. For microplastic examination in sediment, the amount (approximately 1000 g of fresh sediment from each site) was sampled from 15 sites. Fiber microplastics (53%) and fragments (33%) microplastics were predominant. It has shown the abundance and heterogeneity of space. In this study, the highest amount of microplastics detected in sediment samples was related to the size of less than 5000 µm (92%). The frequency of microplastics below 5000 µm in sediment was attributed to the low capacity of existing processes in Ardabil wastewater treatment plant. The abundance of microplastics shows significant differences between sampling sites. The Qara Su River along the city of Ardabil, like many other rivers, is contaminated with microplastics. This study was the first study conducted for evaluation of the sediment environment in terms of the microplastic pollution of Qara Su River in Ardabil. Considering the amount of microplastics in sediments, further research is required to estimate the amount of microplastics released by Ardabil wastewater treatment plants and industrial town wastewater, and other possible sources of emission and to evaluate their contribution to microplastic pollution in water, sediment, and soil. This study provided a framework for future studies of microplastics pollution in the sediment of urban areas around the Qara Su River in Iran.
Nanoscale optical parametric amplification through super-nonlinearity induction
Authors: Aşırım Özüm Emre , Kuzuoğlu Mustafa
Published: July 2022

Optical parametric amplification (OPA) is a nonlinear process through which a low-power input wave is amplified by extracting energy from an interaction medium that is energized by a high-intensity pump wave. For a significant amplification of an input wave, a sufficiently long interaction medium is required, which is usually on the order of a few centimeters. Therefore, in the small scale, OPA is considered unfeasible, and this prevents it from being employed in micro and nanoscale devices. There have been recent studies that proposed microscale OPA through the use of micro-resonators. However, there is currently no study that has suggested high-gain nanoscale OPA, which could be quite useful for implementing nanoscale optical devices. This study aims to show that nanoscale OPA is feasible through the concurrent maximization of the pump wave induced electric energy density and the polarization density (nonlinear coupling strength) within the interaction medium, which enables a very high amount of energy to be transferred to the input wave that is sufficient to amplify the input wave with a gain factor that is comparable to those provided by centimeter scale nonlinear crystals. The computational results of our OPA model match with the experimental ones in the context of sum-harmonic generation, which is the wave-mixing process that gives rise to OPA, with an accuracy of 97.6%. The study aims to make room for further investigation of nanoscale OPA through adaptive optics and/or nonlinear programming algorithms for the enhancement of the process.
Malocclusion in children with speech sound disorders and motor speech involvement: a cross-sectional clinical study in Swedish children
Authors: Mogren Å. , Havner C. , Westerlund A. , Sjögreen L. , Agholme M. Barr , Mcallister A.
Published: July 2022

Objectives

The objectives of this study were to investigate the occurrence, types and severity of malocclusions in children with speech sound disorder (SSD) persisting after 6 years of age, and to compare these findings to a control group of children with typical speech development (TSD).

Methods

In total, 105 children were included: 61 with SSD and motor speech involvement (mean age 8:5 ± 2:8 years; range 6:0–16:7 years, 14 girls and 47 boys) and 44 children with TSD (mean age 8:8 ± 1:6; range 6:0–12:2 years, 19 girls and 25 boys). Extra-oral and intra-oral examinations were performed by an orthodontist. The severity of malocclusion was scored using the IOTN-DHC Index.

Results

There were differences between the SSD and TSD groups with regard to the prevalence, type, and severity of malocclusions; 61% of the children in the SSD group had a malocclusion, as compared to 29% in the TSD group. In addition, the malocclusions in the SSD group were rated as more severe. Functional posterior crossbite and habitual lateral and/or anterior shift appeared more frequently in the SSD group. Class III malocclusion, anterior open bite and scissors bite were found only in the SSD group.

Conclusion

Children with SSD and motor speech involvement are more likely to have a higher prevalence of and more severe malocclusions than children with TSD.
Nutritional risk and its relationship with physical function in community-dwelling older adults
Authors: Bloom Ilse , Zhang Jean , Parsons Camille , Bevilacqua Gregorio , Dennison Elaine M. , Cooper Cyrus , Ward Kate A.
Published: July 2022

Background

Malnutrition is a serious concern in older populations. Simple screening approaches are needed to identify signs of early nutritional risk in older people, to allow intervention before overt malnutrition develops, along with the poorer health outcomes associated with it, such as sarcopaenia and frailty. The main aim of this study was to compare nutrition risk scores, calculated from the DETERMINE Checklist (‘Determine Your Nutritional Health’, also known as the Nutrition Screening Initiative Checklist), with physical function variables in a group of community-dwelling older adults. Another aim was to assess the prevalence of nutrition risk using the DETERMINE and the MUST (Malnutrition Universal Screening Tool).

Methods

Participants of the Hertfordshire Cohort Study (HCS) were recruited and visited at home by a trained researcher. Self-reported physical function was assessed using the SF-36 PF (Short Form-36 Physical Function) scale. The Short Physical Performance Battery (SPPB) was performed, which included the assessment of gait speed, chair rise time and standing balance. Handgrip strength was measured using a Jamar dynamometer. Frailty was assessed according to the presence of at least three of the following Fried frailty criteria: unintentional weight loss, weakness, self-reported exhaustion, slow gait speed and low physical activity. Nutrition risk scores were calculated from the DETERMINE checklist (range 0–21). Nutritional risk was also assessed using the MUST. Analyses were adjusted for sex, age, age left education and number of comorbidities.

Results

In the study, 176 participants (94 men and 82 women), median age 83.3 (IQR 81.5–85.7) years, were assessed. Almost half (47%) scored either ‘moderate’ (score 3–5) or ‘high’ (score ≥ 6) nutritional risk (9% were at high risk), using the DETERMINE checklist, whereas 8% were at risk using the MUST. Higher nutrition risk scores, calculated from DETERMINE, were associated with poorer self-reported physical function (difference in SF-36 PF score: − 0.36, 95% CI (− 0.60, − 0.12) SD per unit increase in nutrition risk score, P = 0.004) and higher odds of being frail (odds ratio Fried frailty: 2.23, 95% CI (1.15, 4.33), P = 0.017). There were no significant associations between DETERMINE nutrition risk scores and the other variables examined.

Conclusion

Cross-sectional associations between higher nutrition risk scores, assessed from the DETERMINE checklist, and poorer self-reported physical function and greater likelihood of frailty suggest that this screening tool may have utility for screening older populations. Prospective studies are required to explore the ability of the tool to predict poor physical function and frailty, though these data suggest it has potential for early, simple detection of nutritional problems in community-living older adults.
Phenotypic Overlap of 22q11.2 Microduplication and Noonan Syndrome in a Fetus with Increased NT, Facial Dysmorphism, and Narrow Pulmonary Trunk
Authors: Nerakh Gayatri , Shah Aditi , Seshan Vyshnavi , Ranganath Prajnya
Published: July 2022

22q11.2 deletion syndrome is a common microdeletion syndrome with a prevalence of 1 in 2000-6000 live births. Clinical features include conotruncal congenital heart defect (CHD), characteristic facial features, neurodevelopmental delay, and immunological abnormalities. Microduplications of 22q11.2 are rare compared to deletions due to ill-defined variable phenotype. A few cases of 22q duplication have been identified in the prenatal period in fetuses with increased nuchal translucency (NT), cardiac anomalies, cleft palate and micrognathia. We report a case of a fetus with increased NT in ultrasound and facial dysmorphism, narrow pulmonary trunk on autopsy evaluation, diagnosed to have microduplication of 22q11.2.
An MPA-based optimized grey Bernoulli model for China’s petroleum consumption forecasting
Authors: Wu Wen-Ze , Hu Zhiming , Qi Qin , Zhang Tao
Published: July 2022

The remarkable prediction of petroleum consumption is of significance for energy scheduling and economic development. Considering the uncertainty and volatility of petroleum system, this paper
presents a nonlinear grey Bernoulli model with combined fractional accumulated generation operator to forecast China’s petroleum consumption and terminal consumption. The newly designed model introduces a combined fractional accumulated generation operator by incorporating the traditional fractional accumulation and conformable fractional accumulation; compared to the old accumulation, the newly optimized accumulation can enhance flexible ability to excavate the development patterns of time-series. In addition, to further improve the prediction performance of the new model, marine predation algorithm is applied to determine the optimal emerging coefficients such as fractional accumulation order. Furthermore, the proposed model is verified by a numerical example of coal consumption; and this newly established model is applied to predict China’s petroleum consumption and terminal consumption. Our tests suggest that the designed ONGBM(1,1,k,c) model outperforms the other benchmark models. Finally, we predict China’s petroleum consumption in the following years with the aid of the optimized model. According to the forecasts of this paper, some suggestions are provided for policy-makers in the relevant sectors.
Critical success factors of construction projects in Jordan: an empirical investigation
Authors: Albtoush A. M. Faten , Doh S. I. , Rahman R. A. , Al-Momani A. H.
Published: July 2022

The construction sector is considered one of the most important engines of the national economy in any country; in addition to that, it clearly contributes to improving the quality of life of individuals. In the construction industry, project success is crucial, because it reflects positively on the growth of the national economy, in partnership with other sectors related to it directly and indirectly. However, construction projects often come with disappointment in completion within time, cost, and quality, for multiple reasons throughout the project life cycle. The aim of this study is to determine the factors that affect the success of construction projects, based on realistic project data. To achieve this goal, data were collected and analyzed from the final reports of a number of projects that had been implemented in 15 years. The result illustrates that the most significant and vital factors for the success of the construction project are: quality-related factors, cost-related factors, time-related factors, contract-related factors, and related external factors. Results help project stakeholders improve construction project performance by identifying factors that have affected project success. This allows them to take appropriate measures for every worker to ensure the success of their projects. In addition, this study contributes to the current body of knowledge by being one of the few studies that analyze project data to identify critical success factors for construction projects in developing countries.
Effects of a trailing-edge flap on stall cell characteristics of a NACA0012 wing
Authors: De Voogt Francis , Ganapathisubramani Bharathram
Published: July 2022

In this case study, we examine the effect of airfoil shape/camber on the formation and existence of stall cells. A series of experiments using a NACA0012 wing with a trailing-edge flap has been carried out over a range of angles of attack (

$11.5^\circ$

–

$21^\circ$

), flap angles (

$0^\circ$

,

$5^\circ$

,

$10^\circ$

) and chord-length-based Reynolds numbers (100 000–500 000). The influence of these parameters on stall cell formation has been explored. Tufts have been used to identify the flow behaviour near the surface, while forces have been measured to relate the surface flow behaviour and wing performance. The results from the tuft analysis on the wing indicated that two different Reynolds-number regimes exist with respect to stall cell formation criteria. A preliminary estimate of the airfoil shape influence on stall cell formation is presented. A data-driven approach is used to relate the aerodynamic parameters (lift coefficient and lift-curve slope) to the formation criteria of stall cells for the wing (and flap angles). The lift coefficient can be used to implicitly take into account the change in airfoil shape in addition to the angle of attack and the Reynolds number. It is hoped that the results presented in this case study could be extended to various other airfoil shapes and that the stall cell formation angle of attack can be deduced from just the mean lift behaviour.
Making the medical mask: surgery, bacteriology, and the control of infection (1870s–1920s)
Authors: Schlich Thomas , Strasser Bruno J.
Published: July 2022

This article examines the introduction of the medical mask in the late nineteenth century at the intersection of surgery, bacteriology and infection control. During this important episode in the longer history of the medical mask, respiratory protection became a tool of targeted germ control. In 1897, the surgeon Johannes Mikulicz at the University of Breslau (now Wroclaw, Poland), drawing on the bacteriological experiments of his colleague Carl Flügge, used a piece of gauze in front of his nose and mouth as a barrier against microorganisms moving from him to his patients. This article explores the social, cultural and medical contexts of this particular use of the mask, in connection with germ theory and surgeons’ struggle with wound infection. It explores the alignment of the new aseptic surgery with the emerging field of bacteriology in a local milieu that favoured interdisciplinary cooperation. The account also follows the uptake of the mask outside of surgery for other anti-infectious purposes and shows how the new type of anti-infectious mask spread simultaneously in operating rooms as well as in hospitals and sanatoria, and eventually in epidemic contexts.
Cannabidiol attenuates insular activity during motivational salience processing in patients with early psychosis
Authors: Gunasekera Brandon , Wilson Robin , O'Neill Aisling , Blest-Hopley Grace , O'Daly Owen , Bhattacharyya Sagnik
Published: July 2022

Background

The mechanisms underlying the antipsychotic potential of cannabidiol (CBD) remain unclear but growing evidence indicates that dysfunction in the insula, a key brain region involved in the processing of motivationally salient stimuli, may have a role in the pathophysiology of psychosis. Here, we investigate whether the antipsychotic mechanisms of CBD are underpinned by their effects on insular activation, known to be involved in salience processing.

Methods

A within-subject, crossover, double-blind, placebo-controlled investigation of 19 healthy controls and 15 participants with early psychosis was conducted. Administration of a single dose of CBD was compared with placebo in psychosis participants while performing the monetary incentive delay task, an fMRI paradigm. Anticipation of reward and loss were used to contrast motivationally salient stimuli against a neutral control condition.

Results

No group differences in brain activation between psychosis patients compared with healthy controls were observed. Attenuation of insula activation was observed following CBD, compared to placebo. Sensitivity analyses controlling for current cannabis use history did not affect the main results.

Conclusion

Our findings are in accordance with existing evidence suggesting that CBD modulates brain regions involved in salience processing. Whether such effects underlie the putative antipsychotic effects of CBD remains to be investigated.
Altered developmental trajectories of verbal learning skills in 22q11.2DS: associations with hippocampal development and psychosis
Authors: Latrèche Caren , Maeder Johanna , Mancini Valentina , Bortolin Karin , Schneider Maude , Eliez Stephan
Published: July 2022

Background

The cognitive profile in 22q11.2 deletion syndrome (22q11.2DS) is often characterized by a discrepancy between nonverbal vs. verbal reasoning skills, in favor of the latter skills. This dissociation has also been observed in memory, with verbal learning skills described as a relative strength. Yet the development of these skills is still to be investigated. We thus aimed to explore verbal learning longitudinally. Furthermore, we explored verbal learning and its respective associations with hippocampal alterations and psychosis, which remain largely unknown despite their high prevalence in 22q11.2DS.

Methods

In total, 332 individuals (173 with 22q11.2DS) aged 5–30 years completed a verbal-paired associates task. Mixed-models regression analyses were conducted to explore developmental trajectories with threefold objectives. First, verbal learning and retention trajectories were compared between 22q11.2DS vs. HC. Second, we examined hippocampal volume development in 22q11.2DS participants with lower vs. higher verbal learning performance. Third, we explored verbal learning trajectories in 22q11.2DS participants with vs. without positive psychotic symptoms and with vs. without a psychotic spectrum disorder (PSD).

Results

Our findings first reveal lower verbal learning performance in 22q11.2DS, with a developmental plateau emerging from adolescence. Second, participants with lower verbal learning scores displayed a reduced left hippocampal tail volume. Third, participants with PSD showed a deterioration of verbal learning performance, independently of verbal reasoning skills.

Conclusion

Our study challenges the current view of preserved verbal learning skills in 22q11.2DS and highlights associations with specific hippocampal alterations. We further identify verbal learning as a novel cognitive marker for psychosis in 22q11.2DS.
English at the center of the periphery: ‘Chicken nuggets’, chronotopes, and scaling English in Bahraini youth
Authors: Al-Alawi Wafa
Published: July 2022

With the spread of English in Bahrain, ‘chicken nugget’ emerged as a term aimed at English-dominant, typically private-school-educated youth. Drawing on data from Bahraini youth, I show how participants orient to different timespaces as they negotiate their identities relative to the ‘chicken nugget’ figure of personhood. Applying discourse analytic methods to participants’ metacommentaries, I demonstrate how they utilize scaling to elevate this label to a fractally recursive bundle of discursive processes, deeming a wider range of people as chicken nuggets depending on the chronotopic conditions of different timespaces. I further show how speakers evoke different exogenous and endogenous styles of English to allow for complex identification processes: the English of chicken nuggets is excessive and exaggerated, as opposed to English as a necessary communication tool in neoliberal contexts. Thus, this article has implications for our understandings of fractal recursivity, English use in globalized contexts, and the sociolinguistics of identity. (Scales and scaling, chronotope, center-periphery, English/Englishes, authenticity, bilingualism, Bahrain, Arabic)*
The economic space of cities: an analysis of leather tanners in eighteenth- and nineteenth-century Rome
Authors: Lelo Keti , Stemperini Giuseppe
Published: July 2022

The aim of this article is to examine the use of suitable archive sources for the study of the economic space of cities in the eighteenth and nineteenth centuries. The research is based on the integration and critical analysis of cartographic, descriptive and quantitative sources of an administrative and fiscal nature, developed by using Geographic Information Systems. The advantage of adopting this approach includes, in addition to the study of spatial relationships amongst economic activities and the urban space, the possibility of investigating discrepancies between the sources, thus facilitating their interpretation.
Primary Care as Primary Target: A Review of Terrorist Attacks Against Primary Care Providers and Their Offices
Authors: Wirken Bart , Barten Dennis G. , De Cauwer Harald , Mortelmans Luc J.M. , Tin Derrick , Cals Jochen
Published: July 2022

Background:

Violence against primary care providers (PCPs) has increased during the current pandemic. While some of these violent acts are not defined as terrorist events, they are intentional events with an aim to disrupt, kill, or injure. Despite their pivotal role in health care, little is known about the risk for PCPs as targets of terrorism.

Methods:

Data collection was performed using a retrospective database search through the Global Terrorism Database (GTD). The GTD was searched using the internal database search functions for all terrorist attacks against PCPs and their offices from January 1, 1970 - December 31, 2019. Years 2020 and 2021 were not yet available at the time of the study. Primary attack and weapon type, location (country, world region), and number of deaths and injuries were collated. Results were exported into an Excel spreadsheet (Microsoft Corp.; Redmond, Washington USA) for analysis.

Results:

There were 29 terrorist attacks against PCPs and their offices from 1970-2019. The majority of attacks occurred during or after 2010. There were 58 fatalities, 52 injured, and 13 hostages. Most documented attacks took place in Pakistan, the United States, and Sri Lanka. Bombings concerned 55% of cases and 21% were hostage-takings.

Conclusion:

Although less common than attacks on other health care related targets, terrorist attacks against PCPs have occurred. The majority of attacks occurred during the last decade. Future studies are warranted to further assess the risk of terrorist attacks against PCPs: before, during, and beyond the current pandemic.
Derivational awareness in late bilinguals increases along with proficiency without a clear influence of the suffixes shared with L1
Authors: Menut Amélie , Brysbaert Marc , Casalis Séverine
Published: July 2022

Morphological awareness contributes to vocabulary acquisition and reading in bilingual children who learned English after their native language. In line with these considerations, we further investigated L2 processing in late adult bilinguals where questions related to morphology need to be clarified. French–English speakers (N = 92) were assessed for three morphological awareness stages: lexical semantic knowledge, syntactic knowledge, and distributive knowledge. We investigated whether the evolution of morphological awareness was related to L2 proficiency and whether it was facilitated by the presence of suffixes shared in L1 and L2. Our results confirmed the influence of language proficiency at each stage of morphological awareness. However, the hypothesis of an advantage of suffixes shared between French and English was not confirmed as no clear advantage was found for those suffixes. Our findings are discussed in line with the morphological congruence hypothesis and compared with the previous results in the literature.
Advancing multimorbidity management in primary care: a narrative review
Authors: Aramrat Chanchanok , Choksomngam Yanee , Jiraporncharoen Wichuda , Wiwatkunupakarn Nutchar , Pinyopornpanish Kanokporn , Mallinson Poppy Alice Carson , Kinra Sanjay , Angkurawaranon Chaisiri
Published: July 2022

Background:

Multimorbidity, defined as the coexistence of two or more chronic conditions in the same individual, is becoming a crucial health issue in primary care. Patients with multimorbidity utilize health care at a higher rate and have higher mortality rates and poorer quality of life compared to patients with single diseases.

Aims:

To explore evidence on how to advance multimorbidity management, with a focus on primary care. Primary care is where a large number of patients with multimorbidity are managed and is considered to be a gatekeeper in many health systems.

Methods:

A narrative review was conducted using four major electronic databases consisting of PubMed, Cochrane, World Health Organization database, and Google scholar. In the first round of reviews, priority was given to review papers summarizing the current issues and challenges in the management of multimorbidity. Thematic analysis using an inductive approach was used to build a framework on how to advance management. The second round of review focused on original articles providing evidence within the primary care context.

Results:

The review found that advancing multimorbidity management in primary care requires a health system approach and a patient-centered approach. The health systems approach includes three major areas: (i) improves access to care, (ii) promotes generalism, and (iii) provides a decision support system. For the patient-centered approach, four key aspects are essential for multimorbidity management: (i) promoting doctor-patient relationship, (ii) prioritizing health problems and sharing decision-making, (iii) supporting self-management, and (iv) integrating care.

Advancement of multimorbidity management in primary care requires integrating concepts of multimorbidity management guidelines with concepts of patient-centered and chronic care models. This simple integration provides an overarching framework for advancing the health care system, connecting the processes of individualized care plans, and integrating care with other providers, family members, and the community.
Promoting Well-Being in Students Through an Art-of-Living Intervention
Authors: Tavakoli Rebekka , Müller Ronja , Jeske Enya , Schäfbuch Nina , Schmitz Bernhard
Published: July 2022

Abstract. During secondary school, students’ well-being is challenged in manifold ways and declines continuously. To address this issue, we designed and evaluated a six-day online art-of-living intervention to foster eighth and ninth graders’ ( N = 69) well-being. Art-of-living (AoL) is based on empirical evidence and conceptualizes strategies that lead to well-being. We tested the effectiveness of the AoL training and investigated the possible contribution of body-related AoL exercises to cognitive exercises by comparing two intervention groups (cognitive training vs. cognitive and body-focused training) and a waitlist control group. Levels of AoL and well-being at pretest, posttest, and two-week follow-up showed that both significantly increased in the intervention groups. No significant differences were found between the cognitive and combined training. We discuss methodological issues of the study and propose that the approach to enhance student well-being by using art-of-living exercises is fruitful for application in school and should be explored further.
Meteorologically‐Informed Spatial Planning of European PV Deployment to Reduce Multiday Generation Variability
Authors: Mühlemann Dirk , Folini Doris , Pfenninger Stefan , Wild Martin , Wohland Jan
Published: July 2022

Renewable generation variability over multiple days is a key challenge in decarbonizing the European power system. Weather regimes are one way to quantify this variability, but so far, their applications to energy research have focused on wind power generation in winter. However, the projected growth of solar photovoltaic (PV) capacity implies that its absolute variability across the continent will grow substantially. Here we combine weather regimes based on ERA5 reanalysis data with country‐specific capacity factors to investigate multiday PV generation variability in Europe. With current installed capacity (131 GW), total PV production in Europe (52.3 GW) varies by 0.9 GW on average, with a maximum change of 3.0 GW, upon transition from one weather regime to another. Using projected PV capacity for 2050 (1.94 TW), variability would rise to 13.9 and 43.8 GW. We present optimized spatial distributions of capacity additions in three scenarios that substantially reduce variability by up to 40%. One of them ascertains a large local PV production, thereby minimizing the need for long‐range power transmission while still reducing variability by about 30%, highlighting that optimized siting and local generation can be reconciled. Our results emphasize the value of leveraging climate information in decarbonizing power systems.
Bipolar Electric Field Pulses in the Martian Magnetosheath and Solar Wind; Their Implication and Impact Accessed by System Scale Size
Authors: Thaller Scott A. , Andersson Laila , Schwartz Steven J. , Mazelle Christian , Fowler Chris , Goodrich Katherine , Newman David , Halekas Jasper , Pilinski Marcin D. , Pollard Matthew
Published: July 2022

The scale size of the plasma boundary region between the sheath and ionosphere in the Martian system is often similar to the gyro‐radii of sheath protons, ∼200 km. As a result, ion energization via kinetic structures may play an important role in modifying the ion trajectories and thus be important when evaluating the large‐scale dynamics of the Martian system. In this paper, we report observations made with the MAVEN Langmuir Probe and Waves instrument of solitary bipolar electric field structures, and assess their potential role in ion energization in the Martian system. The observed structures appear as short duration (∼0.5 ms) bipolar electric field pulses of ∼1–25 mV/m, and are frequently observed in the upstream solar wind and inside the sheath. The study presented in this paper suggests that the bipolar electric field structures observed at Mars have an average electrostatic potential drop of ∼0.07 V. The estimated upper rate at which these structures could further energize the protons is estimated, assuming the protons gain the full 0.07 eV, to be ∼0.13 eV per gyration, or a change in proton energy of ∼0.3%, and a corresponding change in the gyroradius of ∼0.3 km. These numbers imply that to first order the bipolar structures are not a significant source of ion energization in the Martian magnetosheath.
Back‐Projection Imaging of a Tsunami Excitation Area With Ocean‐Bottom Pressure Gauge Array Data
Authors: Mizutani Ayumu , Yomogida Kiyoshi
Published: July 2022

A back‐projection method has been applied to many earthquakes in seismology due to its simple and low computational cost, and it can estimate complex fault rupture processes without any specific a priori information. In this study, we applied the back‐projection method to the tsunami records observed using an ocean‐bottom pressure gauge array and demonstrated it to be a powerful new tool other than the familiar waveform inversion. The obtained back‐projection image was consistent with the initial tsunami height distributions estimated by previous waveform inversions, and its spatial resolution appeared to be even better. Our result suggests that the fault size of the 2016 Off‐Fukushima earthquake was about half, different from the scaling law of standard earthquakes. The present tsunami back‐projection analysis can also estimate the feature of early tsunami propagations. In addition, the estimated image seems to be reliable even 30 min after the origin time, so the back‐projection analysis will be useful in an early detection of the location and spatial extent of a tsunami source. In the present case, the number of available stations in the analysis was found to be affected by the diffraction of tsunami propagation caused by the refraction by a high velocity zone near the Japan Trench. In other words, the further the source is from the coast, the more stations to be analyzed are available. Since most tsunami‐generating earthquakes occur near the subduction axis or its outer‐rise region, the back‐projection analysis should be effective for source estimation of the majority of tsunami‐generating earthquakes.
Dating Low‐Grade Deformation: Role of Lithology and Strain Partitioning on Ar Isotope Records in the Alpi Apuane of Northern Apennines (Italy)
Authors: Di Vincenzo Gianfranco , Godard Gaston , Molli Giancarlo
Published: July 2022

The Alpi Apuane (northern Apennines) represent a key area for understanding the processes related to the Apennines building and geodynamic evolution of the central Mediterranean, but the timing of tectono‐metamorphic events is still debated. White mica from cover rocks of the Massa and Apuane units was investigated by the40Ar‐39Ar in situ and step‐heating techniques. Samples recorded several episodes of deformation developed under low‐grade conditions and resulting in a D1 composite schistosity followed by a variably developed D2 retrograde crenulation. In the Massa Unit, D2 crenulation was accompanied by replacement of muscovite‐phengite by paragonite, whereas the composition of phengite from the Apuane Unit does not vary noticeably, irrespective of whether phengite is aligned along D1 or D2 schistosities. Both units exhibit a significant intrasample scatter of in situ ages and variably discordant age spectra from step‐heating data, due to partial reequilibration of white mica along a deformation path lasting ≥10 Ma, with dates approaching the time of younger events as retrogression becomes more pervasive. The onset of deformation has a minimum age of ∼20 Ma, as documented by40Ar‐39Ar data for the composite D1 foliation in the Massa Unit. D2 deformation shows a common temporal evolution at the scale of the region and is recorded for at least ∼2.5 Ma, starting not after ∼12.5 Ma and ending at ∼10.5 Ma. Results highlight the importance of a detailed microscale characterization and of a careful preselection of samples, as compositionally different domains a few millimeters apart may yield different results because of compositionally driven strain partitioning.
Widely accessible method for 3D microflow mapping at high spatial and temporal resolutions
Authors: Lammertse Evan , Koditala Nikhil , Sauzade Martin , Li Hongxiao , Li Qiang , Anis Luc , Kong Jun , Brouzes Eric
Published: July 2022

Advances in microfluidic technologies rely on engineered 3D flow patterns to manipulate samples at the microscale. However, current methods for mapping flows only provide limited 3D and temporal resolutions or require highly specialized optical set-ups. Here, we present a simple defocusing approach based on brightfield microscopy and open-source software to map micro-flows in 3D at high spatial and temporal resolution. Our workflow is both integrated in ImageJ and modular. We track seed particles in 2D before classifying their Z-position using a reference library. We compare the performance of a traditional cross-correlation method and a deep learning model in performing the classification step. We validate our method on three highly relevant microfluidic examples: a channel step expansion and displacement structures as single-phase flow examples, and droplet microfluidics as a two-phase flow example. First, we elucidate how displacement structures efficiently shift large particles across streamlines. Second, we reveal novel recirculation structures and folding patterns in the internal flow of microfluidic droplets. Our simple and widely accessible brightfield technique generates high-resolution flow maps and it will address the increasing demand for controlling fluids at the microscale by supporting the efficient design of novel microfluidic structures.
The kinase activity of integrin-linked kinase regulates cellular senescence in gastric cancer
Authors: Ji Chengbo , Zhang Mili , Hu Junjie , Cao Can , Gu Qisheng , Liu Youdong , Li Xu , Xu Duogang , Ying Le , Yang Yuqin , Gao Hugh , Li Jikun , Yu Liang
Published: July 2022

The activity of integrin-linked kinase (ILK) in cancerous cells is often oncogenic and associated with malignant properties, such as uncontrolled cell cycle progression and evasion from senescence. However, the role of ILK in cellular senescence in gastric cancer (GC) has not been previously examined. We generated single-cell clones of ILK knock-out using CRISPR-Cas9 in human GC lines with mesenchymal or epithelial histology. Cells with no residual ILK expression exhibited strong cellular senescence with diminished clathrin-mediated endocytosis, Surprisingly, ILK loss-induced cellular senescence appeared to be independent of its function in integrin signaling. The low dose of CPD22, a small molecule inhibitor of ILK activity-induced senescence in three GC cell lines with different histologies. Furthermore, senescent cells with ILK depletion transfected with N-terminal truncated ILK mutant remaining catalytic domains displayed the reduction of senescent phenotypes. RNA sequencing and cytokine array results revealed the enrichment of multiple pro-inflammatory signaling pathways in GC lines in the absence of ILK. Our study identified the important role and the potential mechanism of ILK in the cellular senescence of cancerous epithelial cells. The inhibition of ILK activity using small molecule compounds could have a pro-senescent effect as a therapeutic option for GC.
Association of mitochondrial DNA content, heteroplasmies and inter-generational transmission with autism
Authors: Wang Yiqin , Guo Xiaoxian , Hong Xiumei , Wang Guoying , Pearson Colleen , Zuckerman Barry , Clark Andrew G. , O’Brien Kimberly O. , Wang Xiaobin , Gu Zhenglong
Published: July 2022

Mitochondria are essential for brain development. While previous studies linked dysfunctional mitochondria with autism spectrum disorder (ASD), the role of the mitochondrial genome (mtDNA) in ASD risk is largely unexplored. This study investigates the association of mtDNA heteroplasmies (co-existence of mutated and unmutated mtDNA) and content with ASD, as well as its inter-generational transmission and sex differences among two independent samples: a family-based study (n = 1,938 families with parents, probands and sibling controls) and a prospective birth cohort (n = 997 mother-child pairs). In both samples, predicted pathogenic (PP) heteroplasmies in children are associated with ASD risk (Meta-OR = 1.56, P = 0.00068). Inter-generational transmission of mtDNA reveals attenuated effects of purifying selection on maternal heteroplasmies in children with ASD relative to controls, particularly among males. Among children with ASD and PP heteroplasmies, increased mtDNA content shows benefits for cognition, communication, and behaviors (P ≤ 0.02). These results underscore the value of exploring maternal and newborn mtDNA in ASD.
Development of visual response selectivity in cortical GABAergic interneurons
Authors: Chang Jeremy T. , Fitzpatrick David
Published: July 2022

The visual cortex of carnivores and primates displays a high degree of modular network organization characterized by local clustering and structured long-range correlations of activity and functional properties. Excitatory networks display modular organization before the onset of sensory experience, but the developmental timeline for modular networks of GABAergic interneurons remains under-explored. Using in vivo calcium imaging of the ferret visual cortex, we find evidence that before visual experience, interneurons display weak orientation tuning and widespread, correlated activity in response to visual stimuli. Robust modular organization and orientation tuning are evident with as little as one week of visual experience. Furthermore, we find that the maturation of orientation tuning requires visual experience, while the reduction in widespread, correlated network activity does not. Thus, the maturation of inhibitory cortical networks occurs in a delayed, parallel process relative to excitatory neurons.
Co-optimization of therapeutic antibody affinity and specificity using machine learning models that generalize to novel mutational space
Authors: Makowski Emily K. , Kinnunen Patrick C. , Huang Jie , Wu Lina , Smith Matthew D. , Wang Tiexin , Desai Alec A. , Streu Craig N. , Zhang Yulei , Zupancic Jennifer M. , Schardt John S. , Linderman Jennifer J. , Tessier Peter M.
Published: July 2022

Therapeutic antibody development requires selection and engineering of molecules with high affinity and other drug-like biophysical properties. Co-optimization of multiple antibody properties remains a difficult and time-consuming process that impedes drug development. Here we evaluate the use of machine learning to simplify antibody co-optimization for a clinical-stage antibody (emibetuzumab) that displays high levels of both on-target (antigen) and off-target (non-specific) binding. We mutate sites in the antibody complementarity-determining regions, sort the antibody libraries for high and low levels of affinity and non-specific binding, and deep sequence the enriched libraries. Interestingly, machine learning models trained on datasets with binary labels enable predictions of continuous metrics that are strongly correlated with antibody affinity and non-specific binding. These models illustrate strong tradeoffs between these two properties, as increases in affinity along the co-optimal (Pareto) frontier require progressive reductions in specificity. Notably, models trained with deep learning features enable prediction of novel antibody mutations that co-optimize affinity and specificity beyond what is possible for the original antibody library. These findings demonstrate the power of machine learning models to greatly expand the exploration of novel antibody sequence space and accelerate the development of highly potent, drug-like antibodies.
DDX1 vesicles control calcium-dependent mitochondrial activity in mouse embryos
Authors: Wang Yixiong , Yasmin Lubna , Li Lei , Gao Pinzhang , Xu Xia , Sun Xuejun , Godbout Roseline
Published: July 2022

The DEAD box protein DDX1, previously associated with 3’-end RNA processing and DNA repair, forms large aggregates in the cytoplasm of early mouse embryos. Ddx1 knockout causes stalling of embryos at the 2-4 cell stages. Here, we identify a DDX1-containing membrane-bound calcium-containing organelle with a nucleic acid core. We show that aggregates of these organelles form ring-like structures in early-stage embryos which we have named Membrane Associated RNA-containing Vesicles. We present evidence that DDX1 is required for the formation of Membrane Associated RNA-containing Vesicles which in turn regulate the spatial distribution of calcium in embryos. We find that Ddx1 knockout in early embryos disrupts calcium distribution, and increases mitochondria membrane potential, mitochondrial activity, and reactive oxygen species. Sequencing analysis of embryos from Ddx1 heterozygote crosses reveals downregulation of a subset of RNAs involved in developmental and mitochondrial processes in the embryos with low Ddx1 RNA. We propose a role for Membrane Associated RNA-containing Vesicles in calcium-controlled mitochondrial functions that are essential for embryonic development.