bluuebunny/crossref_metadata_2025 huggingface dataset:
Suggestions for Reviewing Manuscripts
Authors: Alexandrov Andrei V. , Hennerici Michael G. , Norrving Bo
Published: July 2009

Background: Scientific reviewing is a voluntary process to determine if a manuscript deserves publication. REVIEW means: Responsibly Evaluate, Verify and Improve the manuscript, Educate the authors and editors, and Weigh your expert opinion against the submitted work. Provide your review in a respectful, unbiased and timely manner. Review Methods: Make sure editors know about your willingness to review and your particular area(s) of expertise. If you find yourself in a conflict of interest, decline to participate in reviewing. If you accept, complete reviews on time. Determine and rate (1) the methodological validity, (2) originality, (3) significance of findings, (4) the style and clarity of presentation and (5) the findings’ interest to the readership of the journal for which you are asked to review a manuscript. Specifically evaluate (6) if the results support any claims or conclusions made and, most importantly, (7) if the abstract correctly reflects the full content of a manuscript. Summarize your review in specific comments to the authors. Make recommendations whether to accept, revise or reject the manuscript to the editor only. Review Results: Start with a brief summary of the manuscript’s subject, strengths and key findings/claims. Present your specific criticisms and suggestions in numbered lists for the authors to address. Never use demeaning and offensive words or sarcasm since, in the first place, this reflects upon your own ethics and integrity as well as upon the journal’s. Use a constructive tone, and if you see any deficiencies, educate the authors in a respectful manner so that, even if a manuscript is rejected, they will learn from you, improve the manuscript or conduct a better study in the future. Also include ratings from 1 to 7 in your comments to the authors, as far as they are relevant and may explain your final decision. Conclusions: Judge others as you would like to be judged yourself. We hope these suggestions serve to help new reviewers and refresh the willingness of battle-hardened veterans to continuously serve the medical literature.
Phosphorylated Cyclic-AMP-Response Element-Binding Protein and Thyroid Hormone Receptor Have Independent Response Elements in the Rat Thyrotropin-Releasing Hormone Promoter: An Analysis in Hypothalamic Cells
Authors: Díaz-Gallardo M.Y. , Cote-Vélez A. , Carreón-Rodríguez A. , Charli J.L. , Joseph-Bravo P.
Published: July 2009

Background: Thyrotropin-releasing hormone (TRH) from the hypothalamic paraventricular nucleus (PVN) controls the activity of the hypothalamus-pituitary-thyroid axis. TRH is expressed in other hypothalamic nuclei but is downregulated by 3,3′,5-L-triiodothyronine (T3) exclusively in the PVN. Thyroid hormone receptors (TRs) bind TRH promoter at Site-4 (–59/–52), also proposed to bind phosphorylated cAMP response element-binding protein (pCREB). However, nuclear extracts from 8Br-cAMP-stimulated hypothalamic cells showed no binding to Site-4 and instead to cAMP response element (CRE)-2 (–101/–94). Methods: We characterized, by DNA footprinting and chromatin immunoprecipitation, the sites in the rat (–242/+34) TRH promoter that bind to nuclear factors of hypothalamic primary cultures incubated with 8Br-cAMP and/or T3. Results: In primary cultures of fetal hypothalamic cells, TRH mRNA levels rapidly diminished with 10 nM T3 while they increased by 1 mM 8Br-cAMP (± T3). Site-4 was protected from DNase I digestion with nuclear extracts from T3-incubated cells but not from controls or from those incubated with 8Br-cAMP, which protected CRE-2; T3 + 8Br-cAMP coincubation caused no interference. The region protected by nuclear extracts from cAMP-stimulated cells included sequences adjacent to CRE-2-containing response elements of the SP/Krüppel family. A TRβ2 antibody immunoprecipitated chromatin containing Site-4 but not CRE-2, from cells incubated with T3. A pCREB antibody immunoprecipitated CRE-2 containing chromatin in controls and more in 8Br-cAMP-stimulated cells but none when cells were incubated only with T3. Recruitment of the 2 transcription factors was preserved in cells simultaneously exposed to 8Br-cAMP and T3. Discussion: These results show that pCREB binds to a response element in the TRH promoter (CRE-2) that is independent of Site-4 where TRβ2 is bound; pCREB and TR do not present mutual interference on their binding sites.
Sodium Retention in Heart Failure and Cirrhosis
Authors: Bansal Shweta , Lindenfeld JoAnn , Schrier Robert W.
Published: July 2009

Patients with cirrhosis and heart failure (HF) share the pathophysiology of decreased effective arterial blood volume because of splanchnic vasodilatation in cirrhosis and decreased cardiac output in HF, with resultant stimulation of the renin-angiotensin-aldosterone system. Hyperaldosteronism plays a major role in the pathogenesis of ascites and contributes to resistance to loop diuretics. Therefore, the use of high doses of aldosterone antagonist (spironolactone up to 400 mg/day) is the main therapy to produce a negative sodium balance in cirrhotic patients with ascites. Hyperaldosteronism also has increasingly been recognized as a risk factor for myocardial and vascular fibrosis. Therefore, low-dose aldosterone antagonists are being used in patients with HF for cardioprotective action. However, the doses (25 to 50 mg/day) at which they are being used in cardiac patients as reported in the Randomized Aldactone Evaluation Study are not natriuretic. It is likely, therefore, that the mortality benefit relates primarily from their effect on cardiac and vascular fibrosis. Resistance to commonly used loop diuretics is frequently present in patients with advanced HF. In patients with decompensated HF with volume overload who are loop diuretic resistant, ultrafiltration may be the only available option. This is, however, an invasive procedure. For these patients, natriuretic doses of aldosterone antagonists (spironolactone >50 mg/day) may be a potential option. The competitive natriuretic response of aldosterone antagonists is related to activity of the renin-angiotensin-aldosterone system: the higher the renin-angiotensin-aldosterone system activity, the higher the dose of aldosterone antagonist required to produce natriuresis. This article will discuss the potential use of natriuretic doses of aldosterone antagonists in patients with HF, including the potential side effect of hyperkalemia.
Outcomes Research in Cardiovascular Imaging
Authors: Douglas Pamela S. , Taylor Allen , Bild Diane , Bonow Robert , Greenland Philip , Lauer Michael , Peacock Frank , Udelson James
Published: July 2009

In July of 2008, the National Heart, Lung, and Blood Institute convened experts in noninvasive cardiovascular imaging, outcomes research, statistics, and clinical trials to develop recommendations for future randomized controlled trials of the use of imaging in: 1) screening the asymptomatic patient for coronary artery disease; 2) assessment of patients with stable angina; 3) identification of acute coronary syndromes in the emergency room; and 4) assessment of heart failure patients with chronic coronary artery disease with reduced left ventricular ejection fraction. This study highlights several possible trial designs for each clinical situation.
Children and Adults With Congenital Heart Disease Lost to Follow-Up
Authors: Mackie Andrew S. , Ionescu-Ittu Raluca , Therrien Judith , Pilote Louise , Abrahamowicz Michal , Marelli Ariane J.
Published: July 2009

Background—

Many patients with congenital heart disease (CHD) require lifelong care. However, the duration of cardiology follow-up in children and adults with CHD is unknown. We sought to determine the proportion of children and young adults with CHD receiving outpatient cardiology care and to identify predictors of lack of follow-up.

Methods and Results—

The study population consisted of individuals born in 1983 and alive at age 22 years who were diagnosed with CHD in Quebec, Canada, before 6 years of age (n=643). Patients and outpatient visits were identified with the use of the provincial physician’s claims database. Three age groups were examined for the presence of outpatient cardiology follow-up: 6 to 12, 13 to 17, and 18 to 22 years. CHD lesions were classified as severe (n=84; 13%), simple shunts (n=390; 61%), and “other” lesions (n=169; 26%). Failure to receive cardiac follow-up after the 6th, 13th, and 18th birthday occurred in 28%, 47%, and 61%, respectively. Among those with severe lesions, only 79% were seen after the 18th birthday. However, the majority of subjects visited primary care physicians in all age groups, and 93% remained in contact with the healthcare system into early adulthood. Predictors of lack of cardiology follow-up in adulthood included male sex, a nonsevere lesion, and a history of follow-up outside a university hospital setting.

Conclusions—

Lack of cardiology follow-up begins during childhood, even among those with severe lesions. This occurs despite patients being in contact with other healthcare providers. Improved communication with primary care physicians may reduce the proportion of patients lost to cardiac follow-up.
Recurrence of Congenital Heart Defects in Families
Authors: Øyen Nina , Poulsen Gry , Boyd Heather A. , Wohlfahrt Jan , Jensen Peter K.A. , Melbye Mads
Published: July 2009

Background—

Knowledge of the familial contribution to congenital heart diseases (CHD) on an individual and population level is sparse. We estimated an individual’s risk of CHD given a family history of CHD, as well as the contribution of CHD family history to the total number of CHD cases in the population.

Methods and Results—

In a national cohort study, we linked all Danish residents to the National Patient Register, the Causes of Death Register, the Danish Central Cytogenetic Register, and the Danish Family Relations Database, yielding 1 763 591 persons born in Denmark between 1977 and 2005, of whom 18 708 had CHD. Individuals with CHD were classified by phenotype. We estimated recurrence risk ratios and population-attributable risk. Among first-degree relatives, the recurrence risk ratio was 79.1 (95% confidence interval [CI] 32.9 to 190) for heterotaxia, 11.7 (95% CI, 8.0 to 17.0) for conotruncal defects, 24.3 (95% CI,12.2 to 48.7) for atrioventricular septal defect, 12.9 (95% CI, 7.48 to 22.2) for left ventricular outflow tract obstruction, 48.6 (95% CI, 27.5 to 85.6) for right ventricular outflow tract obstruction, 7.1 (95% CI, 4.5 to 11.1) for isolated atrial septal defect, and 3.4 (95% CI, 2.2 to 5.3) for isolated ventricular septal defect. The overall recurrence risk ratio for the same defect was 8.15 (95% CI, 6.95 to 9.55), whereas it was 2.68 (95% CI, 2.43 to 2.97) for different heart defects. Only 2.2% of heart defect cases in the population (4.2% after the exclusion of chromosomal aberrations) were attributed to CHD family history in first-degree relatives.

Conclusions—

Specific CHDs showed highly variable but strong familial clustering in first-degree relatives, ranging from 3-fold to 80-fold compared with the population prevalence, whereas the crossover risks between dissimilar cases of CHD were weaker. Family history of any CHD among first-degree relatives accounted for a small proportion of CHD cases in the population.
Resveratrol Prevents Monocrotaline-Induced Pulmonary Hypertension in Rats
Authors: Csiszar Anna , Labinskyy Nazar , Olson Susan , Pinto John T. , Gupte Sachin , Wu Joseph M. , Hu Furong , Ballabh Praveen , Podlutsky Andrej , Losonczy Gyorgy , de Cabo Rafael , Mathew Rajamma , Wolin Michael S. , Ungvari Zoltan
Published: July 2009

Proliferation of pulmonary arterial smooth muscle cells, endothelial dysfunction, oxidative stress, and inflammation promotes the development of pulmonary hypertension. Resveratrol is a polyphenolic compound that exerts antioxidant and anti-inflammatory protective effects in the systemic circulation, but its effects on pulmonary arteries remain poorly defined. The present study was undertaken to investigate the efficacy of resveratrol to prevent pulmonary hypertension. Rats injected with monocrotaline progressively developed pulmonary hypertension. Resveratrol treatment (25 mg/kg per day, PO, from day 1 postmonocrotaline) attenuated right ventricular systolic pressure and pulmonary arterial remodeling, decreased expression of inflammatory cytokines (tumor necrosis factor-α, interleukin 1β, interleukin 6, and platelet-derived growth factor-α/β), and limited leukocyte infiltration in the lung. Resveratrol also inhibited proliferation of pulmonary arterial smooth muscle cells. Treatment of rats with resveratrol increased expression of endothelial NO synthase, decreased oxidative stress, and improved endothelial function in small pulmonary arteries. Pulmonary hypertension was associated with an upregulation of NAD(P)H oxidase in small pulmonary arteries, which was significantly attenuated by resveratrol treatment. Our studies show that resveratrol exerts anti-inflammatory, antioxidant, and antiproliferative effects in the pulmonary arteries, which may contribute to the prevention of pulmonary hypertension.
Tyrosine Nitration of PA700 Activates the 26S Proteasome to Induce Endothelial Dysfunction in Mice With Angiotensin II–Induced Hypertension
Authors: Xu Jian , Wang Shuangxi , Wu Yong , Song Ping , Zou Ming-Hui
Published: July 2009

The ubiquitin-proteasome system has been implicated in oxidative stress–induced endothelial dysfunction in cardiovascular diseases. However, the mechanism by which oxidative stress alters the ubiquitin-proteasome system is poorly defined. The present study was conducted to determine whether oxidative modifications of PA700, a 26S proteasome regulatory subunit, contributes to angiotensin II (Ang II)–induced endothelial dysfunction. Exposure of human umbilical vein endothelial cells to low concentrations of Ang II, but not vehicle, for 6 hours significantly decreased the levels of tetrahydro-l-biopterin (BH4), an essential cofactor of endothelial NO synthase, which was accompanied by a decrease in GTP cyclohydrolase I, the rate-limiting enzyme for de novo BH4 synthesis. In addition, Ang II increased both tyrosine nitration of PA700 and the 26S proteasome activity, which were paralleled by increased coimmunoprecipitation of PA700 and the 20S proteasome. Genetic inhibition of NAD(P)H oxidase or administration of uric acid (a peroxynitrite scavenger) orNG-nitro-l-arginine methyl ester (nonselective NO synthase inhibitor) significantly attenuated Ang II–induced PA700 nitration, 26S proteasome activation, and reduction of GTP cyclohydrolase I and BH4. Finally, Ang II infusion in mice decreased the levels of both BH4 and GTP cyclohydrolase I and impaired endothelial-dependent relaxation in isolated aortas, and all of these effects were prevented by the administration of MG132, a potent inhibitor for 26S proteasome. We conclude that Ang II increases tyrosine nitration of PA700 resulting in accelerated GTP cyclohydrolase I degradation, BH4 deficiency, and consequent endothelial dysfunction in hypertension.
Aliskiren Enhances the Protective Effects of Valsartan Against Cardiovascular and Renal Injury in Endothelial Nitric Oxide Synthase–Deficient Mice
Authors: Yamamoto Eiichiro , Kataoka Keiichiro , Dong Yi-Fei , Nakamura Taishi , Fukuda Masaya , Tokutomi Yoshiko , Matsuba Shinji , Nako Hisato , Nakagata Naomi , Kaneko Takehito , Ogawa Hisao , Kim-Mitsuyama Shokei
Published: July 2009

The protective effect of aliskiren, a direct renin inhibitor, against hypertensive cardiovascular and renal injury remains to be defined. This study was undertaken to examine the protective effects of the combination of aliskiren and valsartan, an angiotensin receptor blocker, against cardiovascular and renal injury. Endothelial NO synthase–deficient mice, subjected to cuff injury of femoral artery, were divided into 5 groups and were treated with the following: (1) vehicle; (2) aliskiren (25 mg/kg per day); (3) valsartan (8 mg/kg per day); (4) combined aliskiren (12.5 mg/kg per day) and valsartan (4 mg/kg per day); and (5) hydralazine (10 mg/kg per day) for 4 weeks. Aliskiren and valsartan alone markedly and similarly suppressed cardiac hypertrophy, inflammation and fibrosis, and coronary remodeling; prevented cuff injury–induced arterial intimal thickening; and reduced urinary albumin excretion, glomerular inflammation, and glomerulosclerosis in endothelial NO synthase–deficient mice. These beneficial effects of aliskiren and valsartan were associated with the significant attenuation of oxidative stress in these tissues. Hence, aliskiren and valsartan markedly exert the protective effects against cardiovascular and renal injury through the reduction of oxidative stress. Furthermore, compared with monotherapy with aliskiren or valsartan, the combination of a half dose of these drugs more greatly improved the above-mentioned cardiovascular and renal injuries of endothelial NO synthase–deficient mice, which were associated with greater attenuation of tissue oxidative stress by the combination therapy. Thus, the combination of aliskiren and valsartan exerts the synergistic organ-protective effects through synergistic attenuation of oxidative stress. The combination of aliskiren and valsartan seems to be a promising therapeutic strategy for hypertensive organ injury caused by endothelial NO synthase dysfunction.
Pharmacological Characterization of SAR407899, a Novel Rho-Kinase Inhibitor
Authors: Löhn Matthias , Plettenburg Oliver , Ivashchenko Yuri , Kannt Aimo , Hofmeister Armin , Kadereit Dieter , Schaefer Matthias , Linz Wolfgang , Kohlmann Markus , Herbert Jean-Marc , Janiak Philip , O'Connor Stephen E. , Ruetten Hartmut
Published: July 2009

Recent advances in basic and clinical research have identified Rho kinase as an important target potentially implicated in a variety of cardiovascular diseases. Rho kinase is a downstream mediator of RhoA that leads to stress fiber formation, membrane ruffling, smooth muscle contraction, and cell motility. Increased Rho-kinase activity is associated with vasoconstriction and elevated blood pressure. We identified a novel inhibitor of Rho kinase (SAR407899) and characterized its effects in biochemical, cellular, tissue-based, and in vivo assays. SAR407899 is an ATP-competitive Rho-kinase inhibitor, equipotent against human and rat-derived Rho-kinase 2 with inhibition constant values of 36 nM and 41 nM, respectively. It is highly selective in panel of 117 receptor and enzyme targets. SAR407899 is ≈8-fold more active than fasudil. In vitro, SAR407899 demonstrated concentration-dependent inhibition of Rho-kinase–mediated phosphorylation of myosin phosphatase, thrombin-induced stress fiber formation, platelet-derived growth factor–induced proliferation, and monocyte chemotactic protein-1–stimulated chemotaxis. SAR407899 potently (mean IC
50
values: 122 to 280 nM) and species-independently relaxed precontracted isolated arteries of different species and different vascular beds. In vivo, over the dose range 3 to 30 mg/kg PO, SAR407899 lowered blood pressure in a variety of rodent models of arterial hypertension. The antihypertensive effect of SAR407899 was superior to that of fasudil and Y-27632. In conclusion, SAR407899 is a novel and potent selective Rho-kinase inhibitor with promising antihypertensive activity.
Long-Term Environmental Policy: Definition, Knowledge, Future Research
Authors: Sprinz Detlef F.
Published: July 2009

Considering the long-term is not new, yet we seem to be overwhelmed by the long-term nature of many of our environmental policy problems. Following a definition of long-term policy problems, this editorial introduces the contributions to this special issue of Global Environmental Politics and outlines three major challenges for future research, including the time inconsistency problem, the effect of democratic and decentralized governance on problem-solving, as well as institutional designs to prevent or recover from unwanted long-term policy outcomes.
Long-Term Decision-Making: Biological and Psychological Evidence
Authors: Princen Thomas
Published: July 2009

A central conundrum in the need to infuse a long-term perspective into climate policy and other environmental decision-making is the widespread belief that humans are inherently short-term thinkers. An analysis of human decision-making informed by evolved adaptations—biological, psychological and cultural—suggests that humans actually have a long-term thinking capacity. In fact, the human time horizon encompasses both the immediate and the future (near and far term). And yet this very temporal duality makes people susceptible to manipulation; it carries its own politics, a politics of the short term. A “legacy politics” would extend the prevailing time horizon by identifying structural factors that build on evolved biological and cultural factors.
The 419 Code as Business Unusual: Youth and the Unfolding of the Advance Fee Fraud Online Discourse
Authors: Adogame Afe
Published: July 2009

One issue that has launched Nigeria into international réclame and public ignominy, especially in the last decade, is the Advance Fee Fraud (a.k.a. '419'), a code which refers originally to the section of the Nigerian Penal Law that deals with specific fraud schemes. The paper located the economic and socio-political upheavals in Nigeria from the late 1970s as the leverage for the advent and consolidation of fraudulent schemes. It demonstrated how youths have increasingly appropriated the Internet as a gateway to the world and as conduits for information enhancement, economic empowerment or a means to achieving their own ends. Through a careful analysis of 150 documented scam letters, this paper describes the emerging variations of a theme. As a transnational organised crime, this paper demonstrates how its increasing complexity and sophistication, with the target audience of individuals and corporate bodies within the domestic and international frontiers, has attracted local-global security attention and action. The paper concludes that the changing anatomy and the elasticity of the criminal transnational networks can be better grasped, and the preventive measures by individual, government and corporate security agencies prove efficacious, when understood and located within local-global contexts and wider operational frameworks.
Theorising Patriarchy: The Bangladesh Context
Authors: Chowdhury Farah Deeba
Published: July 2009

In Bangladesh, men dominate, oppress and exploit women through private and public patriarchy. Private patriarchy is maintained in the family through the misinterpretation of religion and the non-recognition of unpaid work done by women at home. In the family women are considered as passive dependants and property of their husbands. Women are also excluded from economic and political power through public patriarchy. In the public arena women are only considered as sexual objects and patriarchy is maintained through sexual harassment. Capital accumulation further strengthens patriarchy in Bangladesh. In Bangladesh, men's attitudes towards women are shaped by advertisements, films, beauty contests and pornography where women are used as sexual objects to accumulate capital. Increasingly, men have started to use the dowry system for capital accumulation. Thus men in Bangladesh accumulate capital through private and public patriarchy.
K. Balachander: An Innovative Filmmaker
Authors: Chinniah Sathiavathi
Published: July 2009

This study aims to contribute to existing literature on Indian cinema by exploring the works of K. Balachander. An academic enquiry into K. Balachander's films is justified given his presence in the Tamil film industry for some 40 years and the numerous awards he had obtained over this period for his filmic contributions, including the prestigious Padmashree Award which he won in 1987. In the bigger study of which this is a small part, I also study female representation in his films, as well as how various groups of audience interpret the portrayal of women in three specific films directed by him. Keeping in mind the varied audience groups of Tamil cinema, three different groups have been selected for this purpose, namely the rural and urban audiences in India and the diaspora audience in Singapore. Emphasis on the audience allows this study to build on earlier research on the reception of films at the point of consumption. I hope in this to provide a new perspective on audience reception of female portrayals in Tamil films.
East India Company in Sumatra: Cross-Cultural Interactions
Authors: Silva Jayasuriya Shihan de
Published: July 2009

For scholars concerned with historical studies of cross-continental movement, migration from Africa to Asia poses challenges. Administrative records of the East India Company reveal the multi-ethnicity of slaves, trends of slavery, resistance to slavery and the circumstances that led to emancipation of the slaves. Through a case study on Sumatra, this paper considers how transition from British to Dutch control affected the emancipated slaves, what rights they had and their eventual fate. It suggests that descendants of African slaves could still be living in Southeast Asia although creolisation and assimilation may have rendered them invisible.
Cross-Cultural Influences on the Language of the Sri Lankan Malays
Authors: Silva Jayasuriya Shihan de
Published: July 2009

Although Sri Lanka has had links with the Malays from ancient times, the Sri Lankan Malays trace their ancestry only from the mid-seventeenth century. Taking into account the process of global commercial interactions and territorial expansion, this paper demonstrates the effects of cross-cultural contact in the language of the Sri Lanka Malays illustrated through typological alterations and lexical changes in a multilingual and multicultural setting.
The Myth of the Sultans in the Western Indian Ocean during the Nineteenth Century: A New Hypothesis
Authors: Nicolini Beatrice
Published: July 2009

The power of the Al Bu Sa'id Sultans of Oman was widely known as based on delicate balances of forces (and ethnic-social groups), deeply different among them. In fact, the elements that composed the nineteenth century Omani leadership were, and had always been, generally 'divided' amongst three different ethnic groups: the Baluch, the Asian merchant communities and the African regional leaders (Mwiny Mkuu). Within this framework, the role played by European Powers, particularly by the Treaties signed between the Sultans of Oman and the East India Company for abolishing slavery, and by the arms trade was crucial for the development of the Gulf and the Western Indian Ocean international networks They highly contributed to the gradual 'shifting' of the Omanis from the slave trade to clove and spice cultivation – the major economic source of Zanzibar Island – along the coastal area of Sub-Saharan East Africa. The role played by the Omani Sultans – the myth – within the western traditional historiography, which often described them as firmly controlling both the Arabian and African littorals and the major trading ports of the Western Indian Ocean during the nineteenth century, will be reexamined in this paper, taking into account recent research studies and international debates in the topic. The new hypothesis consists of a different perception of the concepts of power and control (political and territorial) of the Western Indian Ocean littorals by the most famous of the Sultans of Oman during the nineteenth century: Saiyid Sa'id bin Sultan Al Bu Sa'id.
Eurasian Women as Tawa'if Singers and Recording Artists: Entertainment and Identity-making in Colonial India
Authors: Sachdeva Jha Shweta
Published: July 2009

Scholarship on Eurasians has often addressed issues of migration, collective identity and debates around home. Women performers however do not find themselves discussed in these histories of Eurasian peoples in India. This paper aims to account for individual agency in shaping one's identity within the meta-narratives of collective identity of migrant peoples. I focus on two Eurasian women entertainers in the colonial cities of Benares and Calcutta who chose to forget their mixed-race past to fashion successful careers using new identities as tawa'if singers and actors in the late nineteenth and early twentieth centuries. This, I shall argue, was possible within the wider context of emergent colonial modernities in colonial India. By choosing micro-level case histories of these celebrity entertainers, I want to argue for including popular culture as an arena of identity-making within histories of migration and gender. To engage with popular culture, I shall extend our perception of historical 'archive' to include a varied set of materials such as biographical anecdotes, discographies, songbooks, and address the fields of poetry, music and history. Through this project I hope to rethink ideas of gender, culture and agency within wider debates of migration and identity-making.
From Ocean to Lakes: Cultural Transformations of Yemenis in Kenya and Uganda
Authors: Beckerleg Susan
Published: July 2009

Migration from Yemen to East Africa has been occurring for centuries and continued well into the twentieth century. Since the European explorations of the nineteenth century the term 'Arab-Swahili', as distinguished from 'African', has been in use. The ways that Yemenis have both adopted and changed Swahili culture in Kenya are outlined in this paper. Most Yemeni migrants who settled in Uganda passed through Mombasa, acquiring some knowledge of the Swahili language en route. However, the Yemenis of Uganda are not Swahili, despite using the Swahili language as a major medium of communication, even at home. Ugandan 'Arab' food eaten at home and cooked by Yemenis in cafes is actually Indian/Swahili cuisine. The ways that Yemenis have promoted the cultivation of qat across Uganda and have made its consumption a marker of identity are described. The degree that the terminology of diaspora studies can be applied to Yemenis in Kenya and Uganda is assessed, and concludes that the migrants are both 'cultural hybrids' and 'transnationals'.
Cytokine-dependent and–independent gene expression changes and cell cycle block revealed in Trypanosoma cruzi-infected host cells by comparative mRNA profiling
Authors: Costales Jaime A , Daily Johanna P , Burleigh Barbara A
Published: July 2009

Background

The requirements for growth and survival of the intracellular pathogen Trypanosoma cruzi within mammalian host cells are poorly understood. Transcriptional profiling of the host cell response to infection serves as a rapid read-out for perturbation of host physiology that, in part, reflects adaptation to the infective process. Using Affymetrix oligonucleotide array analysis we identified common and disparate host cell responses triggered by T. cruzi infection of phenotypically diverse human cell types.

Results

We report significant changes in transcript abundance in T. cruzi-infected fibroblasts, endothelial cells and smooth muscle cells (2852, 2155 and 531 genes respectively; fold-change ≥ 2, p-value < 0.01) 24 hours post-invasion. A prominent type I interferon response was observed in each cell type, reflecting a secondary response to secreted cytokine in infected cultures. To identify a core cytokine-independent response in T. cruzi-infected fibroblasts and endothelial cells transwell plates were used to distinguish cytokine-dependent and -independent gene expression profiles. This approach revealed the induction of metabolic and signaling pathways involved in cell proliferation, amino acid catabolism and response to wounding as common themes in T. cruzi-infected cells. In addition, the downregulation of genes involved in mitotic cell cycle and cell division predicted that T. cruzi infection may impede host cell cycle progression. The observation of impaired cytokinesis in T. cruzi-infected cells, following nuclear replication, confirmed this prediction.

Conclusion

Metabolic pathways and cellular processes were identified as significantly altered at the transcriptional level in response to T. cruzi infection in a cytokine-independent manner. Several of these alterations are supported by previous studies of T. cruzi metabolic requirements or effects on the host. However, our methods also revealed a T. cruzi-dependent block in the host cell cycle, at the level of cytokinesis, previously unrecognized for this pathogen-host cell interaction.
MHC-linked and un-linked class I genes in the wallaby
Authors: Siddle Hannah V , Deakin Janine E , Coggill Penny , Hart Elizabeth , Cheng Yuanyuan , Wong Emily SW , Harrow Jennifer , Beck Stephan , Belov Katherine
Published: July 2009

Background

MHC class I antigens are encoded by a rapidly evolving gene family comprising classical and non-classical genes that are found in all vertebrates and involved in diverse immune functions. However, there is a fundamental difference between the organization of class I genes in mammals and non-mammals. Non-mammals have a single classical gene responsible for antigen presentation, which is linked to the antigen processing genes, including TAP. This organization allows co-evolution of advantageous class Ia/TAP haplotypes. In contrast, mammals have multiple classical genes within the MHC, which are separated from the antigen processing genes by class III genes. It has been hypothesized that separation of classical class I genes from antigen processing genes in mammals allowed them to duplicate. We investigated this hypothesis by characterizing the class I genes of the tammar wallaby, a model marsupial that has a novel MHC organization, with class I genes located within the MHC and 10 other chromosomal locations.

Results

Sequence analysis of 14 BACs containing 15 class I genes revealed that nine class I genes, including one to three classical class I, are not linked to the MHC but are scattered throughout the genome. Kangaroo Endogenous Retroviruses (KERVs) were identified flanking the MHC un-linked class I. The wallaby MHC contains four non-classical class I, interspersed with antigen processing genes. Clear orthologs of non-classical class I are conserved in distant marsupial lineages.

Conclusion

We demonstrate that classical class I genes are not linked to antigen processing genes in the wallaby and provide evidence that retroviral elements were involved in their movement. The presence of retroviral elements most likely facilitated the formation of recombination hotspots and subsequent diversification of class I genes. The classical class I have moved away from antigen processing genes in eutherian mammals and the wallaby independently, but both lineages appear to have benefited from this loss of linkage by increasing the number of classical genes, perhaps enabling response to a wider range of pathogens. The discovery of non-classical orthologs between distantly related marsupial species is unusual for the rapidly evolving class I genes and may indicate an important marsupial specific function.
Characterization and isolation of a T-DNA tagged banana promoter active during in vitro culture and low temperature stress
Authors: Santos Efrén , Remy Serge , Thiry Els , Windelinckx Saskia , Swennen Rony , Sági László
Published: July 2009

Background

Next-generation transgenic plants will require a more precise regulation of transgene expression, preferably under the control of native promoters. A genome-wide T-DNA tagging strategy was therefore performed for the identification and characterization of novel banana promoters. Embryogenic cell suspensions of a plantain-type banana were transformed with a promoterless, codon-optimized luciferase (luc
+) gene and low temperature-responsive luciferase activation was monitored in real time.

Results

Around 16,000 transgenic cell colonies were screened for baseline luciferase activity at room temperature 2 months after transformation. After discarding positive colonies, cultures were re-screened in real-time at 26°C followed by a gradual decrease to 8°C. The baseline activation frequency was 0.98%, while the frequency of low temperature-responsive luciferase activity was 0.61% in the same population of cell cultures. Transgenic colonies with luciferase activity responsive to low temperature were regenerated to plantlets and luciferase expression patterns monitored during different regeneration stages. Twenty four banana DNA sequences flanking the right T-DNA borders in seven independent lines were cloned via PCR walking. RT-PCR analysis in one line containing five inserts allowed the identification of the sequence that had activated luciferase expression under low temperature stress in a developmentally regulated manner. This activating sequence was fused to the uidA reporter gene and back-transformed into a commercial dessert banana cultivar, in which its original expression pattern was confirmed.

Conclusion

This promoter tagging and real-time screening platform proved valuable for the identification of novel promoters and genes in banana and for monitoring expression patterns throughout in vitro development and low temperature treatment. Combination of PCR walking techniques was efficient for the isolation of candidate promoters even in a multicopy T-DNA line. Qualitative and quantitative GUS expression analyses of one tagged promoter in a commercial cultivar demonstrated a reproducible promoter activity pattern during in vitro culture. Thus, this promoter could be used during in vitro selection and generation of commercial transgenic plants.
Strategies for achieving a high response rate in a home interview survey
Authors: Kiezebrink Kirsty , Crombie Iain K , Irvine Linda , Swanson Vivien , Power Kevin , Wrieden Wendy L , Slane Peter W
Published: July 2009

Background

Response rates in surveys have been falling over the last 20 years, leading to the need for novel approaches to enhance recruitment. This study describes strategies used to maximise recruitment to a home interview survey of mothers with young children living in areas of high deprivation.

Methods

Mothers of two year old children received a letter from their GP inviting them to take part in a survey on diet. Participants were subsequently recruited by a researcher. The researcher first tried to contact potential participants by telephone, to discuss the study and make an appointment to conduct a home interview. Where telephone numbers for women could not be obtained from GP records, web searches of publicly available databases were conducted. After obtaining correct telephone numbers, up to six attempts were made to establish contact by telephone. If this was unsuccessful, a postal request for telephone contact was made. Where no telephone contact was achieved, the researcher sent up to two appointments by post to conduct a home interview.

Results

Participating GPs invited 372 women to take part in a home based interview study. GP practices provided telephone numbers for 162 women, of which 134 were valid numbers. The researcher identified a further 187 numbers from electronic directories. Further searches of GP records by practice staff yielded another 38 telephone numbers. Thus, telephone numbers were obtained for 99% of potential participants.

The recruitment rate from telephone contacts was 77%. Most of the gain was achieved within four calls. For the remaining women, contact by post and home visits resulted in 18 further interviews, corresponding to 35% of the women not recruited by telephone. The final interview rate was 82%. This was possible because personal contact was established with 95% of potential participants.

Conclusion

This study achieved a high response rate in a hard to reach group. This was mainly achieved by first establishing contact by telephone. The use of multiple sources identified the telephone numbers of almost all the sample. Multiple attempts at telephone contact followed by postal approaches led to a high home interview rate.
TNF-α-induced up-regulation of pro-inflammatory cytokines is reduced by phosphatidylcholine in intestinal epithelial cells
Authors: Treede Irina , Braun Annika , Jeliaskova Petia , Giese Thomas , Füllekrug Joachim , Griffiths Gareth , Stremmel Wolfgang , Ehehalt Robert
Published: July 2009

Background

Phosphatidylcholine (PC) is a major lipid of the gastrointestinal mucus layer. We recently showed that mucus from patients suffering from ulcerative colitis has low levels of PC. Clinical studies reveal that the therapeutic addition of PC to the colonic mucus using slow release preparations is beneficial. The positive role of PC in this disease is still unclear; however, we have recently shown that PC has an intrinsic anti-inflammatory property. It could be demonstrated that the exogenous application of PC inhibits membrane-dependent actin assembly and TNF-α-induced nuclear NF-κB activation. We investigate here in more detail the hypothesis that the exogenous application of PC has anti-inflammatory properties.

Methods

PC species with different fatty acid side chains were applied to differentiated and non-differentiated Caco-2 cells treated with TNF-α to induce a pro-inflammatory response. We analysed TNF-α-induced NF-κB-activation via the transient expression of a NF-κB-luciferase reporter system. Pro-inflammatory gene transcription was detected with the help of a quantitative real time (RT)-PCR analysis. We assessed the binding of TNF-α to its receptor by FACS and analysed lipid rafts by isolating detergent resistant membranes (DRMs).

Results

The exogenous addition of all PC species tested significantly inhibited TNF-α-induced pro-inflammatory signalling. The expression levels of IL-8, ICAM-1, IP-10, MCP-1, TNF-α and MMP-1 were significantly reduced after PC pre-treatment for at least two hours. The effect was comparable to the inhibition of NF-kB by the NF-kB inhibitor SN 50 and was not due to a reduced binding of TNF-α to its receptor or a decreased surface expression of TNF-α receptors. PC was also effective when applied to the apical side of polarised Caco-2 cultures if cells were stimulated from the basolateral side. PC treatment changed the compartmentation of the TNF-α-receptors 1 and 2 to DRMs.

Conclusion

PC induces a prolonged inhibition of TNF-α-induced pro-inflammatory signalling. This inhibition may be caused by a shift of the TNF-α receptors at the surface to lipid rafts. Our results may offer a potential molecular explanation for the positive role of PC seen in clinical studies for the treatment of ulcerative colitis.
Certification of breast centres in Germany: proof of concept for a prototypical example of quality assurance in multidisciplinary cancer care
Authors: Brucker Sara Y , Bamberg Michael , Jonat Walter , Beckmann Matthias W , Kämmerle Andreas , Kreienberg Rolf , Wallwiener Diethelm
Published: July 2009

Background

The main study objectives were: to develop a set of requirements of comprehensive breast centres; to establish a nationwide voluntary certification programme for breast centres based on such requirements, a certified quality management system (QMS), and scheduled independent, external audits and periodic recertification; and to demonstrate the general acceptance of such a certification programme with a view to introducing similar certification programmes for other major cancers.

Methods

Breast centres introduced a QMS and voluntarily participated in an external certification procedure based on guideline-derived Requirements of Breast Centres specifically developed for the application procedure, all subsequent audits and recertification. All data (numbers of pending and successful applications, sites/centre, etc.) were collected by a newly founded, independent organisation for certification of cancer services delivery. Data analysis was descriptive.

Results

Requirements of Breast Centres were developed by the German Cancer Society (DKG), the German Society of Senology (DGS) and other relevant specialist medical societies in the form of a questionnaire comprising 185 essential items based on evidence-based guidelines and the European Society of Breast Cancer Specialists' (EUSOMA) requirements of specialist breast units. From late 2002 to mid 2008, the number of participating breast centres rose from 1 to 175. As of mid 2008, 77% of an estimated 50,000 new breast cancers in Germany were diagnosed and treated at certified breast centres, 78% of which were single-site centres.

Conclusion

Nationwide voluntary certification of breast centres is feasible and well accepted in Germany. Dual certification of breast centres that involves certification of breast services to guideline-derived requirements in conjunction with independent certification of a mandatory QMS can serve as a model for other multidisciplinary site-specific cancer centres.
The consumption of Internet child pornography and violent and sex offending
Authors: Endrass Jérôme , Urbaniok Frank , Hammermeister Lea C , Benz Christian , Elbert Thomas , Laubacher Arja , Rossegger Astrid
Published: July 2009

Background

There is an ongoing debate on whether consumers of child pornography pose a risk for hands-on sex offenses. Up until now, there have been very few studies which have analyzed the association between the consumption of child pornography and the subsequent perpetration of hands-on sex offenses. The aim of this study was to examine the recidivism rates for hands-on and hands-off sex offenses in a sample of child pornography users using a 6 year follow-up design.

Methods

The current study population consisted of 231 men, who were subsequently charged with consumption of illegal pornographic material after being detected by a special operation against Internet child pornography, conducted by the Swiss police in 2002. Criminal history, as well as recidivism, was assessed using the criminal records from 2008.

Results

4.8% (n = 11) of the study sample had a prior conviction for a sexual and/or violent offense, 1% (n = 2) for a hands-on sex offense, involving child sexual abuse, 3.3% (n = 8) for a hands-off sex offense and one for a nonsexual violent offense. When applying a broad definition of recidivism, which included ongoing investigations, charges and convictions, 3% (n = 7) of the study sample recidivated with a violent and/or sex offense, 3.9% (n = 9) with a hands-off sex offense and 0.8% (n = 2) with a hands-on sex offense.

Conclusion

Consuming child pornography alone is not a risk factor for committing hands-on sex offenses – at least not for those subjects who had never committed a hands-on sex offense. The majority of the investigated consumers had no previous convictions for hands-on sex offenses. For those offenders, the prognosis for hands-on sex offenses, as well as for recidivism with child pornography, is favorable.