bluuebunny/crossref_metadata_2025 huggingface dataset:
Hepatic alveolar echinococcosis: correlation between computed tomography morphology and inflammatory activity in positron emission tomography
Authors: Graeter Tilmann , Eberhardt Nina , Shi Rong , Schmidberger Julian , Beer Ambros J. , Beer Meinrad , Henne-Bruns Doris , Hillenbrand Andreas , Barth Thomas F. E. , Grimm Johannes , Kratzer Wolfgang , Gruener Beate
Published: July 2020

Positron emission tomography-computed tomography (PET-CT) with 18F-fluorodesoxyglucose (FDG) is the imaging modality of choice for assessing inflammation surrounding hepatic alveolar echinococcosis (AE) lesions. This study is the first to evaluate FDG uptake in hepatic AE (n = 51) based on the standardized uptake value (SUV) and to correlate the SUVs with primary morphology and calcification patterns, based on the Echinococcus multilocularis Ulm Classification for Computed-Tomography (EMUC-CT). Our results show that the SUVs were increased for lesions with EMUC-CT types I-IV primary morphology, compared to the surrounding healthy liver tissue (SUV = 2.5 ± 0.4; p < 0.05). Type IV lesions included, by far, the highest number of PET-negative lesions. A comparison of lesions with different primary morphologies showed clear differences. The highest SUVs were found for types I and III, and the lowest was found for type IV. Type IV lesions (SUV, 3.8 ± 1.5) showed significantly lower uptake compared to type I (SUV, 6.9 ± 3.5; p = 0.030) and type III (SUV, 7.4 ± 3.9; p = 0.031) lesions. For type II lesions, the results showed only a statistical trend (SUV, 6.1 ± 3.1; p = 0.073). Due to the small number of cases, an evaluation of type V (n = 1) lesions was not possible. The different SUVs of lesions with different primary morphologies, particularly the lower FDG uptake observed in type IV lesions, suggested that these SUVs might reflect different stages of the disease.
Evolution and forecasting of PM10 concentration at the Port of Gijon (Spain)
Authors: Sánchez Lasheras Fernando , García Nieto Paulino José , García Gonzalo Esperanza , Bonavera Laura , de Cos Juez Francisco Javier
Published: July 2020

The name PM10refers to small particles with a diameter of less than 10 microns. The present research analyses different models capable of predicting PM10concentration using the previous values of PM10, SO2, NO, NO2, CO and O3as input variables. The information for model training uses data from January 2010 to December 2017. The models trained were autoregressive integrated moving average (ARIMA), vector autoregressive moving average (VARMA), multilayer perceptron neural networks (MLP), support vector machines as regressor (SVMR) and multivariate adaptive regression splines. Predictions were performed from 1 to 6 months in advance. The performance of the different models was measured in terms of root mean squared errors (RMSE). For forecasting 1 month ahead, the best results were obtained with the help of a SVMR model of six variables that gave a RMSE of 4.2649, but MLP results were very close, with a RMSE value of 4.3402. In the case of forecasts 6 months in advance, the best results correspond to an MLP model of six variables with a RMSE of 6.0873 followed by a SVMR also with six variables that gave an RMSE result of 6.1010. For forecasts both 1 and 6 months ahead, ARIMA outperformed VARMA models.
Robust phenotypic maintenance of limb cells during heterogeneous culture in a physiologically relevant polymeric-based constructed graft system
Authors: Barajaa Mohammed A. , Nair Lakshmi S. , Laurencin Cato T.
Published: July 2020

A major challenge during the simultaneous regeneration of multiple tissues is the ability to maintain the phenotypic characteristics of distinct cell populations on one construct, especially in the presence of different exogenous soluble cues such as growth factors. Therefore, in this study, we questioned whether phenotypic maintenance over a distinct population of cells can be achieved by providing biomimetic structural cues relevant to each cell phenotype into the construct’s design and controlling the presentation of growth factors in a region-specific manner. To address this question, we developed a polymeric-based constructed graft system (CGS) as a physiologically relevant model that consists of three combined regions with distinct microstructures and growth factor types. Regions A and B of the CGS exhibited similar microstructures to the skin and soft tissues and contained rhPDGF-BB and rhIGF-I, while region C exhibited a similar microstructure to the bone tissue and contained rhBMP-2. Primary rat skin fibroblasts, soft tissue fibroblasts, and osteoblasts were then cultured on regions A, B, and C of the CGS, respectively and their phenotypic characteristics were evaluated in this heterogenous environment. In the absence of growth factors, we found that the structural cues presented in every region played a key role in maintaining the region-specific cell functions and heterogeneity during a heterogeneous culture. In the presence of growth factors, we found that spatially localizing the growth factors at their respective regions resulted in enhanced region-specific cell functions and maintained region-specific cell heterogeneity compared to supplementation, which resulted in a significant reduction of cell growth and loss of phenotype. Our data suggest that providing biomimetic structural cues relevant to each cell phenotype and controlling the presentation of growth factors play a crucial role in ensuring heterogeneity maintenance of distinct cell populations during a heterogeneous culture. The presented CGS herein provides a reliable platform for investigating different cells responses to heterogeneous culture in a physiologically relevant microenvironment. In addition, the model provides a unique platform for evaluating the feasibility and efficacy of different approaches for simultaneously delivering multiple growth factors or molecules from a single construct to achieve enhanced cell response while maintaining cellular heterogeneity during a heterogenous culture.
Individually customized transcranial temporal interference stimulation for focused modulation of deep brain structures: a simulation study with different head models
Authors: Lee Sangjun , Lee Chany , Park Jimin , Im Chang-Hwan
Published: July 2020

Temporal interference (TI) stimulation was recently proposed that allows for the stimulation of deep brain structures with neocortical regions being minimally stimulated. For human brain modulation, TI current patterns are known to be considerably affected by the complex structures of the human head, and thus, it is hard to deliver TI current to a specific deep brain region. In this study, we optimized scalp electrode configurations and injection currents that can deliver maximum TI stimulation currents to a specific deep brain region, the head of the right hippocampus in this study, considering the real anatomical head structures of each individual. Three realistic finite element (FE) head models were employed for the optimization of TI stimulation. To generate TI current patterns, two pairs of scalp electrodes were selected, which carry two sinusoidally alternating currents with a small frequency difference. For every possible combination of electrode pairs, optimal injection currents delivering the maximal TI currents to the head of the right hippocampus were determined. The distribution of the optimized TI currents was then compared with that of the unoptimized TI currents and the conventional single frequency alternating current stimulation. Optimization of TI stimulation parameters allows for the delivery of the desired amount of TI current to the target region while effectively reducing the TI currents delivered to cortical regions compared to the other stimulation approaches. Inconsistency of the optimal stimulation conditions suggest that customized stimulation, considering the individual anatomical differences, is necessary for more effective transcranial TI stimulation. Customized transcranial TI stimulation based on the numerical field analysis is expected to enhance the overall effectiveness of noninvasive stimulation of the human deep brain structures.
Identification of spin effects in the anomalous Righi–Leduc effect in ferromagnetic metals
Authors: Zhou Da-Kun , Xu Qing-Lian , Yu Xiao-Qin , Zhu Zhen-Gang , Su Gang
Published: July 2020

The emerging of spin caloritronics leads to a series of new spin-thermal related effects, such as spin Seebeck effect (SSE), spin Nernst effect (SNE) and their corresponding inverse effects. Anomalous Righi–Leduc effect (ARLE) describes that a transverse temperature gradient can be induced by a longitudinal heat flow in ferromagnets. The driving force and the response of the ARLE are all involved with heat. It is curious if spin effects mediate the heat transport and provide extra influence. In this work, we investigate the ARLE and the interplay between the heat current, charge current, and spin current via linear response theory. We identified that spin effects do have clear roles in heat transport, which can be confirmed by phase shifts of voltage output varying with the direction of magnetization. Our formulas fit the experimental data very well. Moreover, we discuss more configuration of magnetization which is expected to be tested in the future. It should be emphasized that the present formalism including spin effects is out of the theory based on magnon transport, which may be conspicuous in the devices within the spin diffusion length.
Investigation of molecular mechanisms of experimental compounds in murine models of chronic allergic airways disease using synchrotron Fourier-transform infrared microspectroscopy
Authors: Mazarakis Nadia , Vongsvivut Jitraporn , Bambery Keith R. , Ververis Katherine , Tobin Mark J. , Royce Simon G. , Samuel Chrishan S. , Snibson Kenneth J. , Licciardi Paul V. , Karagiannis Tom C.
Published: July 2020

The ovalbumin-induced (OVA) chronic allergic airways murine model is a well-established model for investigating pre-clinical therapies for chronic allergic airways diseases, such as asthma. Here, we examined the effects of several experimental compounds with potential anti-asthmatic effects including resveratrol (RV), relaxin (RLN), l-sulforaphane (LSF), valproic acid (VPA), and trichostatin A (TSA) using both a prevention and reversal model of chronic allergic airways disease. We undertook a novel analytical approach using focal plane array (FPA) and synchrotron Fourier-transform infrared (S-FTIR) microspectroscopic techniques to provide new insights into the mechanisms of action of these experimental compounds. Apart from the typical biological effects, S-FTIR microspectroscopy was able to detect changes in nucleic acids and protein acetylation. Further, we validated the reduction in collagen deposition induced by each experimental compound evaluated. Although this has previously been observed with conventional histological methods, the S-FTIR technique has the advantage of allowing identification of the type of collagen present. More generally, our findings highlight the potential utility of S-FTIR and FPA-FTIR imaging techniques in enabling a better mechanistic understanding of novel asthma therapeutics.
Novel susceptibility loci for A(H7N9) infection identified by next generation sequencing and functional analysis
Authors: Zhao Baihui , Chen Yongkun , Li Mo , Zhou Jianfang , Teng Zheng , Chen Jian , Zhao Xue , Wu Hao , Bai Tian , Mao Shenghua , Fang Fanghao , Chu Wei , Huang Hailiang , Huai Cong , Shen Lu , Zhou Wei , Sun Liangdan , Zheng Xiaodong , Cheng Guangxia , Sun Ye , Wang Dayan , He Lin , Shu Yuelong , Zhang Xi , Qin Shengying
Published: July 2020

The A(H7N9) virus strain that emerged in 2013 was associated with a high fatality rate and may become a long-term threat to public health. A(H7N9) disease incidence is disproportionate to viral exposure, suggesting that host genetic factors may significantly influence susceptibility to A(H7N9) infection. Human genome variation in conferring risk for A(H7N9) infection in Chinese populations was identified by a two-stage investigation involving 121 A(H7N9) patients and 187 healthy controls using next generation sequencing followed by functional analysis. As a result, a low frequency variant (rs189256251; P = 0.0303, OR = 3.45, 95% CI 1.05–11.35, chi-square test) and three HLA alleles (DQB1*06:01, DQA1*05:05 and C*12:02) were identified in A(H7N9) infected volunteers. In an A549 cell line carrying the rs189256251 variant CT genotype, A(H7N9) infection incidence was elevated 6.665-fold over control cells carrying the CC genotype. Serum levels of interferon alpha were significantly lower in patients with the CT genotype compared to the CC genotype (P = 0.01). The study findings of genetic predisposition to A(H7N9) in the Chinese population may be valuable in systematic investigations of A(H7N9) disease etiology.
The novel features of Plantago ovata seed mucilage accumulation, storage and release
Authors: Phan Jana L. , Cowley James M. , Neumann Kylie A. , Herliana Lina , O’Donovan Lisa A. , Burton Rachel A.
Published: July 2020

Seed mucilage polysaccharide production, storage and release in Plantagoovata is strikingly different to that of the model plant Arabidopsis. We have used microscopy techniques to track the development of mucilage secretory cells and demonstrate that mature P.ovata seeds do not have an outer intact cell layer within which the polysaccharides surround internal columellae. Instead, dehydrated mucilage is spread in a thin homogenous layer over the entire seed surface and upon wetting expands directly outwards, away from the seed. Observing mucilage expansion in real time combined with compositional analysis allowed mucilage layer definition and the roles they play in mucilage release and architecture upon hydration to be explored. The first emergent layer of hydrated mucilage is rich in pectin, extremely hydrophilic, and forms an expansion front that functions to ‘jumpstart’ hydration and swelling of the second layer. This next layer, comprising the bulk of the expanded seed mucilage, is predominantly composed of heteroxylan and appears to provide much of the structural integrity. Our results indicate that the synthesis, deposition, desiccation, and final storage position of mucilage polysaccharides must be carefully orchestrated, although many of these processes are not yet fully defined and vary widely between myxospermous plant species.
Genetic variation in the farnesoid X-receptor predicts Crohn’s disease severity in female patients
Authors: Wilson Aze , Wang Qian , Almousa Ahmed A. , Jansen Laura E. , Choi Yun-hee , Schwarz Ute I. , Kim Richard B.
Published: July 2020

The farnesoid X receptor (FXR) is implicated in Crohn's disease (CD) pathogenesis. It is unclear how genetic variation in FXR impacts CD severity versus genetic variation in nuclear receptors such as pregnane X receptor (PXR) and the multi-drug resistance protein 1 (MDR1, ABCB1). To evaluate FXR-1G > T as a genomic biomarker of severity in CD and propose a plausible molecular mechanism. A retrospective study (n = 542) was conducted in a Canadian cohort of CD patients. Genotypic analysis (FXR-1G > T, MDR1 3435C > T and PXR -25385C > T) as well as determination of the FXR downstream product, fibroblast growth factor (FGF) 19 was performed. Primary outcomes included risk and time to first CD-related surgery. The effect of estrogen on wild type and variant FXR activity was assessed in HepG2 cells. The FXR-1GT genotype was associated with the risk of (odds ratio, OR = 3.34, 95% CI = 1.58–7.05, p = 0.002) and earlier progression to surgery (hazard ratio, HR = 3.00, 95% CI = 1.86–4.83, p < 0.0001) in CD. Female carriers of the FXR-1GT genotype had the greatest risk of surgery (OR = 14.87 95% CI = 4.22–52.38, p < 0.0001) and early progression to surgery (HR = 6.28, 95% CI = 3.62–10.90, p < 0.0001). Women carriers of FXR-1GT polymorphism had a three-fold lower FGF19 plasma concentration versus women with FXR-1GG genotype (p < 0.0001). In HepG2 cells cotransfected with estrogen receptor (ER) and FXR, presence of estradiol further attenuated variant FXR activity. MDR1 and PXR genotypes were not associated with surgical risk. Unlike MDR1 and PXR, FXR-1GT genetic variation is associated with earlier and more frequent surgery in women with CD. This may be through ER-mediated attenuation of FXR activation.
Computational study of the unimolecular and bimolecular decomposition mechanisms of propylamine
Authors: Almatarneh Mansour H. , Al Omari Rima , Omeir Reema A. , Al Khawaldeh Ahmad , Afaneh Akef T. , Sinnokrot Mutasem , Al Akhras Alaa , Marashdeh Ali
Published: July 2020

A detailed computational study of the dehydrogenation reaction of trans-propylamine (trans-PA) in the gas phase has been performed using density functional method (DFT) and CBS-QB3 calculations. Different mechanistic pathways were studied for the reaction of n-propylamine. Both thermodynamic functions and activation parameters were calculated for all investigated pathways. Most of the dehydrogenation reaction mechanisms occur in a concerted step transition state as an exothermic process. The mechanisms for pathways A and B comprise two key-steps: H2 eliminated from PA leading to the formation of allylamine that undergoes an unimolecular dissociation in the second step of the mechanism. Among these pathways, the formation of ethyl cyanide and H2 is the most significant one (pathway B), both kinetically and thermodynamically, with an energy barrier of 416 kJ mol−1. The individual mechanisms for the pathways from C to N involve the dehydrogenation reaction of PA via hydrogen ion, ammonia ion and methyl cation. The formation of α-propylamine cation and NH3 (pathway E) is the most favorable reaction with an activation barrier of 1 kJ mol−1. This pathway has the lowest activation energy calculated of all proposed pathways.
Comparative physiological and root proteome analyses of two sorghum varieties responding to water limitation
Authors: Goche Tatenda , Shargie Nemera G. , Cummins Ian , Brown Adrian P. , Chivasa Stephen , Ngara Rudo
Published: July 2020

When exposed to drought stress many plants reprogram their gene expression to activate adaptive biochemical and physiological responses for survival. However, most of the well-studied adaptive responses are common between drought-sensitive and drought-tolerant species, making it difficult to identify the key mechanisms underpinning successful drought tolerance in crops. We developed a sorghum experimental system that compares between drought-sensitive (ICSB338) and enhanced drought-tolerant (SA1441) varieties. We show that sorghum activates a swift and robust stomatal shutdown to preserve leaf water content when water stress has been sensed. Water uptake is enhanced via increasing root cell water potential through the rapid biosynthesis of predominantly glycine betaine and an increased root-to-shoot ratio to explore more soil volume for water. In addition to stomatal responses, there is a prompt accumulation of proline in leaves and effective protection of chlorophyll during periods of water limitation. Root and stomatal functions rapidly recover from water limitation (within 24 h of re-watering) in the drought-tolerant variety, but recovery is impaired in the drought-sensitive sorghum variety. Analysis of the root proteome revealed complex protein networks that possibly underpin sorghum responses to water limitation. Common and unique protein changes between the two sorghum varieties provide new targets for future use in investigating sorghum drought tolerance.
Optical phonons of SnSe(1−x)Sx layered semiconductor alloys
Authors: Sriv Tharith , Nguyen Thi Minh Hai , Lee Yangjin , Lim Soo Yeon , Nguyen Van Quang , Kim Kwanpyo , Cho Sunglae , Cheong Hyeonsik
Published: July 2020

The evolution of the optical phonons in layered semiconductor alloys SnSe(1–x)Sx is studied as a function of the composition by using polarized Raman spectroscopy with six different excitation wavelengths (784.8, 632.8, 532, 514.5, 488, and 441.6 nm). The polarization dependences of the phonon modes are compared with transmission electron diffraction measurements to determine the crystallographic orientation of the samples. Some of the Raman modes show significant variation in their polarization behavior depending on the excitation wavelengths. It is established that the maximum intensity direction of the Ag2 mode of SnSe(1−x)Sx (0 ≤ x ≤ 1) does not depend on the excitation wavelength and corresponds to the armchair direction. It is additionally found that the lower-frequency Raman modes of Ag1, Ag2 and B3g1 in the alloys show the typical one-mode behavior of optical phonons, whereas the higher-frequency modes of B3g2, Ag3 and Ag4 show two-mode behavior.
Does fertilization with dehydrated sewage sludge affect Terminalia argentea (Combretaceae) and associated arthropods community in a degraded area?
Authors: Carvalho Jó Cássio Nascimento , Silva Farley William Souza , Leite Germano Leão Demolin , Azevedo Alcinei Mistico , Teixeira Gustavo Leal , Soares Marcus Alvarenga , Zanuncio José Cola , Legaspi Jesusa Crisostomo
Published: July 2020

Nutrients from dehydrated sewage sludge play an essential role in the development of many plants such as Terminalia argentea, in the recovery of degraded areas. The aims were to assess the abundance, diversity and species richness of phytophagous, pollinators and predators arthropods, as well as the percentage of defoliation of T. argentea trees, fertilized (or not) with dehydrated sewage sludge in a degraded area. The abundance, diversity and species richness of phytophagous Coleoptera and total predators (predator insects + protocooperating ants + spiders); abundance and species richness of Diptera, pollinator insects, spiders, and predators (predator insects + spiders) were higher on trees fertilized with dehydrated sewage sludge. The abundance of phytophagous Coleoptera declined with the presence of phytophagous Hemiptera and protocooperating ants; population of phytophagous Orthoptera declined in response to phytophagous Coleoptera and total predators; the numbers of the leafminer Lyriomyza sp. directly increased with the numbers of spiders. The ecological indices of phytophagous, pollinators, and predator arthopods increased on Terminalia argentea trees fertilized with dehydrated sewage sludge; such a better ecological indices in fertilized than in unfertilized trees, show it more suitable for the recovery of degraded areas. We discuss the competition between phytophagous insects groups as well as herbivory reduction by predators.
A deep learning model for detecting mental illness from user content on social media
Authors: Kim Jina , Lee Jieon , Park Eunil , Han Jinyoung
Published: July 2020

Users of social media often share their feelings or emotional states through their posts. In this study, we developed a deep learning model to identify a user’s mental state based on his/her posting information. To this end, we collected posts from mental health communities in Reddit. By analyzing and learning posting information written by users, our proposed model could accurately identify whether a user’s post belongs to a specific mental disorder, including depression, anxiety, bipolar, borderline personality disorder, schizophrenia, and autism. We believe our model can help identify potential sufferers with mental illness based on their posts. This study further discusses the implication of our proposed model, which can serve as a supplementary tool for monitoring mental health states of individuals who frequently use social media.
Monodisperse palladium–cobalt alloy nanocatalyst supported on activated carbon (AC) as highly effective catalyst for the DMAB dehydrocoupling
Authors: Sen Betul , Acidereli Hilal , Karaman Neslihan , Sen Fatih
Published: July 2020

In the study, activated carbon (AC) supported palladium/cobalt (Pd/Co) nanocatalyst was synthesized to achieve hydrogen release from dimethylamine boron (DMAB). Nanocatalyst were produced by the reduction of Pd2+ and Co2+ cations by the ultrasonic double reduction method. Analytical studies of the synthesized nanomaterials were characterized by X-ray photoelectron spectroscopy, Raman spectroscopy, X-ray diffraction, transmission electron microscopy (TEM), high-resolution transmission electron microscopy (HR-TEM), electron energy loss spectroscopy, ultraviolet–visible spectroscopy. In this research, nanomaterials exhibited high catalytic activity and reusability, and great performance at low temperatures and concentrations. For the dehydrogenation reaction of dimethylamine borane, TOF and Ea were calculated as 379.5 h−1 and 75.86 kJ mol−1, respectively. The PdCo@AC nanocatalyst can be used as a promising catalyst for the hydrogen production reaction from DMAB.
Rationale for the design of 3D-printable bioresorbable tissue-engineering chambers to promote the growth of adipose tissue
Authors: Faglin Pierre , Gradwohl Marion , Depoortere César , Germain Nicolas , Drucbert Anne-Sophie , Brun Stéphanie , Nahon Claire , Dekiouk Salim , Rech Alexandre , Azaroual Nathalie , Maboudou Patrice , Payen Julien , Danzé Pierre-Marie , Guerreschi Pierre , Marchetti Philippe
Published: July 2020

Tissue engineering chambers (TECs) bring great hope in regenerative medicine as they allow the growth of adipose tissue for soft tissue reconstruction. To date, a wide range of TEC prototypes are available with different conceptions and volumes. Here, we addressed the influence of TEC design on fat flap growth in vivo as well as the possibility of using bioresorbable polymers for optimum TEC conception. In rats, adipose tissue growth is quicker under perforated TEC printed in polylactic acid than non-perforated ones (growth difference 3 to 5 times greater within 90 days). Histological analysis reveals the presence of viable adipocytes under a moderate (less than 15% of the flap volume) fibrous capsule infiltrated with CD68+ inflammatory cells. CD31-positive vascular cells are more abundant at the peripheral zone than in the central part of the fat flap. Cells in the TEC exhibit a specific metabolic profile of functional adipocytes identified by 1H-NMR. Regardless of the percentage of TEC porosity, the presence of a flat base allowed the growth of a larger fat volume (p < 0.05) as evidenced by MRI images. In pigs, bioresorbable TEC in poly[1,4-dioxane-2,5-dione] (polyglycolic acid) PURASORB PGS allows fat flap growth up to 75 000 mm3 at day 90, (corresponding to more than a 140% volume increase) while at the same time the TEC is largely resorbed. No systemic inflammatory response was observed. Histologically, the expansion of adipose tissue resulted mainly from an increase in the number of adipocytes rather than cell hypertrophy. Adipose tissue is surrounded by perfused blood vessels and encased in a thin fibrous connective tissue containing patches of CD163+ inflammatory cells. Our large preclinical evaluation defined the appropriate design for 3D-printable bioresorbable TECs and thus opens perspectives for further clinical applications.
Spectrochemical analysis in blood plasma combined with subsequent chemometrics for fibromyalgia detection
Authors: Passos João Octávio Sales , dos Santos Alves Marcelo Victor , Morais Camilo L. M. , Martin Francis L. , Cavalcante Antônio Felipe , Lemos Telma Maria Araújo Moura , Moura Shayanne , Freitas Daniel L. D. , Mariz João Vitor Medeiros , Carvalho Jean Lucas , Lima Kássio M. G. , Pegado Rodrigo
Published: July 2020

Fibromyalgia is a rheumatologic condition characterized by multiple and chronic body pain, and other typical symptoms such as intense fatigue, anxiety and depression. It is a very complex disease where treatment is often made by non-medicated alternatives in order to alleviate symptoms and improve the patient’s quality of life. Herein, we propose a method to detect patients with fibromyalgia (n = 252, 126 controls and 126 patients with fibromyalgia) through the analysis of their blood plasma using attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy in conjunction with chemometric techniques, hence, providing a low-cost, fast and accurate diagnostic approach. Different chemometric algorithms were tested to classify the spectral data; genetic algorithm with linear discriminant analysis (GA-LDA) achieved the best diagnostic results with a sensitivity of 89.5% in an external test set. The GA-LDA model identified 24 spectral wavenumbers responsible for class separation; amongst these, the Amide II (1,545 cm−1) and proteins (1,425 cm−1) were identified to be discriminant features. These results reinforce the potential of ATR-FTIR spectroscopy with multivariate analysis as a new tool to screen and detect patients with fibromyalgia in a fast, low-cost, non-destructive and minimally invasive fashion.
The DMCdrive: practical 3D-printable micro-drive system for reliable chronic multi-tetrode recording and optogenetic application in freely behaving rodents
Authors: Kim Hoseok , Brünner Hans Sperup , Carlén Marie
Published: July 2020

Electrophysiological recording and optogenetic control of neuronal activity in behaving animals have been integral to the elucidation of how neurons and circuits modulate network activity in the encoding and causation of behavior. However, most current electrophysiological methods require substantial economical investments and prior expertise. Further, the inclusion of optogenetics with electrophysiological recordings in freely moving animals adds complexity to the experimental design. Expansion of the technological repertoire across laboratories, research institutes, and countries, demands open access to high-quality devices that can be built with little prior expertise from easily accessible parts of low cost. We here present an affordable, truly easy-to-assemble micro-drive for electrophysiology in combination with optogenetics in freely moving rodents. The DMCdrive is particularly suited for reliable recordings of neurons and network activities over the course of weeks, and simplify optical tagging and manipulation of neurons in the recorded brain region. The highly functional and practical drive design has been optimized for accurate tetrode movement in brain tissue, and remarkably reduced build time. We provide a complete overview of the drive design, its assembly and use, and proof-of-principle demonstration of recordings paired with cell-type-specific optogenetic manipulations in the prefrontal cortex (PFC) of freely moving transgenic mice and rats.
Proteomics analysis of FUS mutant human motoneurons reveals altered regulation of cytoskeleton and other ALS-linked proteins via 3′UTR binding
Authors: Garone Maria Giovanna , Alfano Vincenzo , Salvatori Beatrice , Braccia Clarissa , Peruzzi Giovanna , Colantoni Alessio , Bozzoni Irene , Armirotti Andrea , Rosa Alessandro
Published: July 2020

Increasing evidence suggests that in Amyotrophic Lateral Sclerosis (ALS) mutated RNA binding proteins acquire aberrant functions, leading to altered RNA metabolism with significant impact on encoded protein levels. Here, by taking advantage of a human induced pluripotent stem cell-based model, we aimed to gain insights on the impact of ALS mutant FUS on the motoneuron proteome. Label-free proteomics analysis by mass-spectrometry revealed upregulation of proteins involved in catabolic processes and oxidation–reduction, and downregulation of cytoskeletal proteins and factors directing neuron projection. Mechanistically, proteome alteration does not correlate with transcriptome changes. Rather, we observed a strong correlation with selective binding of mutant FUS to target mRNAs in their 3′UTR. Novel validated targets, selectively bound by mutant FUS, include genes previously involved in familial or sporadic ALS, such as VCP, and regulators of membrane trafficking and cytoskeleton remodeling, such as ASAP1. These findings unveil a novel mechanism by which mutant FUS might intersect other pathogenic pathways in ALS patients’ motoneurons.
Evaluations of effects of sleep surfaces on athletic performance in youth
Authors: Maruyama Takashi , Sato Shinichi , Matsumura Mari , Ono Taisuke , Nishida Masaki , Nishino Seiji
Published: July 2020

We recently demonstrated that sleeping on high rebound [HR] mattress toppers induced a continuous and more rapid decline in core body temperature compared to low rebound [LR] mattress toppers during the initial phase of nocturnal sleep in young healthy volunteers. HR toppers are characterized by their supportive feel and high breathability whereas LR toppers are pressure-absorbing. In the current study, we evaluated effects of HR mattress toppers on objectively-(actigraphy) and subjectively-(questionnaires) evaluated sleep, vigilance (psychomotor vigilance test), and athletic performance (40-m sprint time, long jump distance, and star drill time) in youth male athletes age 10–19, in two sessions: fifty-one subjects in 2013 (study I) and 23 subjects in 2014 (study II). Sleeping on HR mattress toppers for four to six weeks improved some athletic performance measures compared to sleeping on LR or sleeping directly on spring mattresses without a topper. Statistically significant improvements in 40-m sprint time in study I (compared to LR) and in star drill time in study II (no topper) were observed. No changes in sleep and psychomotor vigilance were observed. These results suggest selecting optimal sleep surfaces may contribute to the maximization of athletic performances, and further studies are warranted.
Evaluation of the microencapsulation of orange essential oil in biopolymers by using a spray-drying process
Authors: Aguiar Maria Clara Santana , das Graças Fernandes da Silva Maria Fátima , Fernandes João Batista , Forim Moacir Rossi
Published: July 2020

Essential oils are volatile compounds commonly used by several industries, easily degradable, which restrains their applications. Therefore, we developed and validated a methodology for producing microcapsules loaded with orange essential oil, using a spray-drying process. The experimental design results showed that the combination between a low flow transfer rate (0.15 L h−1) of the colloidal suspension, a higher drying air flow rate (536 L h−1), and an inlet air temperature of 150 °C to the spray-dryer were the most important parameters for the atomization efficiency. The method optimization resulted in microcapsules with powder recovery between 7.6 and 79.9% (w w−1), oil content ranging from 8.9 to 90.4% (w w−1), encapsulation efficiency between 5.7 and 97.0% (w w−1), and particle sizes with a high frequency of distribution less than 4 μm. In these experiments, gelatin and lignin were evaluated as biopolymers of encapsulation. We also developed an analytical method using headspace gas chromatography. The matrix effects could be addressed by using matrix-matched calibration curves. The chromatographic analysis was linear and selective for d-limonene between 0.025 and 3.00 µg mL−1, with correlation coefficients higher than 0.99. The analytical method had limits of detection and quantitation of 0.024 and 0.073 mg g−1 for gelatin and 0.039 and 0.119 mg g−1 for lignin, respectively.
Differential impact of thermal and physical permafrost disturbances on High Arctic dissolved and particulate fluvial fluxes
Authors: Beel C. R. , Lamoureux S. F. , Orwin J. F. , Pope M. A. , Lafrenière M. J. , Scott N. A.
Published: July 2020

Climate warming and changing precipitation patterns have thermally (active layer deepening) and physically (permafrost-thaw related mass movements) disturbed permafrost-underlain watersheds across much of the Arctic, increasing the transfer of dissolved and particulate material from terrestrial to aquatic ecosystems. We examined the multiyear (2006–2017) impact of thermal and physical permafrost disturbances on all of the major components of fluvial flux. Thermal disturbances increased the flux of dissolved organic carbon (DOC), but localized physical disturbances decreased multiyear DOC flux. Physical disturbances increased major ion and suspended sediment flux, which remained elevated a decade after disturbance, and changed carbon export from a DOC to a particulate organic carbon (POC) dominated system. As the magnitude and frequency of physical permafrost disturbance intensifies in response to Arctic climate change, disturbances will become an increasingly important mechanism to deliver POC from terrestrial to aquatic ecosystems. Although nival runoff remained the primary hydrological driver, the importance of pluvial runoff as driver of fluvial flux increased following both thermal and physical permafrost disturbance. We conclude the transition from a nival-dominated fluvial regime to a regime where rainfall runoff is proportionately more important will be a likely tipping point to accelerated High Arctic change.
Short term association between air pollution (PM10, NO2 and O3) and secondary spontaneous pneumothorax
Authors: Marx Tania , Bernard Nadine , Parmentier Anne-Laure , Puyraveau Marc , Martin Berenger , Gantelet Madeleine , Pretalli Jean-Baptiste , Dalphin Jean-Charles , Mauny Frédéric , Desmettre Thibaut
Published: July 2020

Secondary spontaneous pneumothorax (SSP) occurs in the context of underlying pulmonary disease. Our objectives were to estimate the relationship between SSP and short term air pollution exposure with nitrogen dioxide (NO2), ozone (O3) and particulate matter with a diameter ≤ 10 μm (PM10). Patients with SSP were included between June 1, 2009 and May 31, 2013, in 14 Emergency Departments in France. In this case–crossover design study, PM10,NO2, and O3data were collected hourly from monitoring stations. Quantitative values, fast increase in air pollutant concentration, and air quality threshold exceedance were retained. These assessments were calculated for each of the 4 days prior to the event (Lag 1–Lag 4) for all case and control period, and for the entire exposure period. A total of 135 patients with SSP were included, with a mean age of 55.56 (SD 18.54) years. For short term exposure of PM10, NO2and O3, no differences were observed between case and control periods in terms of quantitative values of air pollutant exposure (P > 0.68), fast increase in concentration (P > 0.12) or air quality threshold exceedance (P > 0.68). An association between O3exposures cannot be ruled out, especially when considering the Lag 2 prior to the event and in warm seasons.
Hydrogen–nitrogen plasma assisted synthesis of titanium dioxide with enhanced performance as anode for sodium ion batteries
Authors: Wang Hongmei , Xiong Jie , Cheng Xing , Chen Ge , Kups Thomas , Wang Dong , Schaaf Peter
Published: July 2020

Sodium ion batteries are considered as one of the most promising energy storage devices as lithium ion batteries due to the natural abundance of sodium. TiO2 is very popular as anode materials for both lithium and sodium ion batteries because of the nontoxicity, safety and great stabilities. However, the low electronic conductivities and inferior sodium ion diffusion make it becoming a great challenge to develop advanced TiO2 anodes. Doping heteroatoms and incorporation of defects are believed to be great ways to improve the electrochemical performance of TiO2 anodes. In this work, commercial TiO2 (P25) nanoparticles was modified by hydrogen and nitrogen high-power plasma resulting in a disordered surface layer formation and nitrogen doping as well. The electrochemical performances of the samples as anode materials for sodium ion batteries was measured and the results indicated that after the hydrogen–nitrogen plasma treatment, H–N-TiO2 electrode shows a 43.5% of capacity higher than the P-TiO2 after 400 cycles long-term discharge/charge process, and the samples show a good long cycling stability as well, the Coulombic efficiencies of all samples are nearly 99% after 50 cycles which could be sustained to the end of long cycling. In addition, hydrogen–nitrogen plasma treated TiO2 electrode reached the stable high Coulombic efficiency earlier than the pristine material. High resolution TEM images and XPS results indicate that there is a disordered surface layer formed after the plasma treatment, by which defects (oxygen vacancies) and N-doping are also introduced into the crystalline structure. All these contribute to the enhancement of the electrochemical performance.
Environmental DNA detection tracks established seasonal occurrence of blacktip sharks (Carcharhinus limbatus) in a semi-enclosed subtropical bay
Authors: Postaire Bautisse D. , Bakker Judith , Gardiner Jayne , Wiley Tonya R. , Chapman Demian D.
Published: July 2020

The integration of eDNA analysis into the population assessment and monitoring of sharks could greatly improve temporal and spatial data used for management purposes. This study aimed to compare eDNA detection against well-established seasonal changes in blacktip shark (Carcharhinus limbatus) abundance in Terra Ceia Bay (FL, USA). We used a species-specific real-time PCR approach to detect C. limbatus eDNA in the bay on a near monthly basis from spring through mid-fall in 2018 and 2019. Previous studies have shown that C. limbatus give birth in the bay in early summer and immature sharks occur there until late fall, when decreasing water temperatures cause them to move offshore and southwards. Water samples (2 L) were collected (4–6 per month) and filtered in the field, with each then being subjected to real-time PCR. Carcharhinus limbatus ‘positive’ filters were significantly more commonly collected during the April-July sampling period than during the August-October sampling period. While following the predicted pattern, eDNA concentration was generally too low for accurate quantification. Our results show that C. limbatus eDNA detection follows known seasonal residency patterns consistently over 2 years of monitoring. Species-specific eDNA analysis using real-time PCR could therefore represent a cost-effective, scalable sampling tool to facilitate improved shark population monitoring in semi-enclosed marine habitats.
A fully automated artificial intelligence method for non-invasive, imaging-based identification of genetic alterations in glioblastomas
Authors: Calabrese Evan , Villanueva-Meyer Javier E. , Cha Soonmee
Published: July 2020

Glioblastoma is the most common malignant brain parenchymal tumor yet remains challenging to treat. The current standard of care—resection and chemoradiation—is limited in part due to the genetic heterogeneity of glioblastoma. Previous studies have identified several tumor genetic biomarkers that are frequently present in glioblastoma and can alter clinical management. Currently, genetic biomarker status is confirmed with tissue sampling, which is costly and only available after tumor resection or biopsy. The purpose of this study was to evaluate a fully automated artificial intelligence approach for predicting the status of several common glioblastoma genetic biomarkers on preoperative MRI. We retrospectively analyzed multisequence preoperative brain MRI from 199 adult patients with glioblastoma who subsequently underwent tumor resection and genetic testing. Radiomics features extracted from fully automated deep learning-based tumor segmentations were used to predict nine common glioblastoma genetic biomarkers with random forest regression. The proposed fully automated method was useful for predicting IDH mutations (sensitivity = 0.93, specificity = 0.88), ATRX mutations (sensitivity = 0.94, specificity = 0.92), chromosome 7/10 aneuploidies (sensitivity = 0.90, specificity = 0.88), and CDKN2 family mutations (sensitivity = 0.76, specificity = 0.86).
Factors controlling the distributions of dissolved organic matter in the East China Sea during summer
Authors: Kim Jeonghyun , Kim Tae-Hoon , Park Sang Rul , Lee Hyuk Je , Kim Jang Kyun
Published: July 2020

To determine the distribution of dissolved organic matter (DOM) in the East China Sea (ECS) during the summer, we measured the dissolved organic carbon (DOC) and nitrogen (DON), fluorescent dissolved organic matter (FDOM), and chlorophyll a (Chl. a) in the upper 100-m layer of this region during July and September 2015. The DOC (r2 = 0.72 and 0.78 in July and September, respectively) and DON (r2 = 0.43 and 0.33) were significantly correlated with salinity, suggesting that the river is the primary origin of DOM. However, we found that at a DOC “pulse” under a salinity ranging from 24 to 35, the extrapolating DOC values (304 ± 11 μM) were twice higher than those with a salinity of close to 0, as found in a previous study. The excess DOC concentration seemed to be attributed to the microbial metabolism during transport from the estuary based on the good relationships between DOC and marine humic-like FDOM (r2 = 0.42 and 0.47), as well as the fluorescence, humification, and biological indexes, but showed no correlation with Chl. a. Thus, the results of our study indicate that microbial activities can be a significant factor controlling the distribution of DOM in the ECS during summer.
Facile formulation and fabrication of the cathode using a self-lithiated carbon for all-solid-state batteries
Authors: Delaporte N. , Darwiche A. , Léonard M. , Lajoie G. , Demers H. , Clément D. , Veillette R. , Rodrigue L. , Trudeau M. L. , Kim C. , Zaghib K.
Published: July 2020

We propose a innovative concept to boost the electrochemical performance of cathode composite electrodes using surface-modified carbons with hydrophilic moieties to increase their dispersion in a Lithium Nickel Manganese Cobalt Oxide (NMC) cathode and in-situ generate Li-rich carbon surfaces. Using a rapid aqueous process, the hydrophilic carbon is effectively dispersed in NMC particles followed by the conversion of its acid surface groups (e.g. –COOH), which interact with the NMC particles due to their basicity, into grafted Li salt (–COO−Li+). The solid-state batteries prepared using the cathode composites with surface-modified carbon exhibit better electrochemical performance. Such modified carbons led to a better electronic conduction path as well as facilitating Li+ ions transfer at the carbon/NMC interface due to the presence of lithiated carboxylate groups on their surface.
Scale-dependent particle diffusivity and apparent viscosity in polymer solutions as probed by dynamic magnetic nanorheology
Authors: Hess Melissa , Gratz Micha , Remmer Hilke , Webers Samira , Landers Joachim , Borin Dmitry , Ludwig Frank , Wende Heiko , Odenbach Stefan , Tschöpe Andreas , Schmidt Annette M.
Published: July 2020

A systematic survey on magnetic particle nanorheology on polymer solutions using different particle sizes and geometries, and different driving modes.
A new porous coordination polymer reveals selective sensing of Fe3+, Cr2O72−, CrO42−, MnO4− and nitrobenzene, and stimuli-responsive luminescence color conversions
Authors: Li Bin , Yan Qing-Qing , Yong Guo-Ping
Published: July 2020

A novel blue-emitting luminescent porous coordination polymer reveals excellent luminescence sensing properties for the detection of different analytes, and stimuli-responsive conversion between the crystal (blue light) and its amorphous form (green light).
Hybrid spherical nucleotide nanoparticles can enhance the synergistic anti-tumor effect of CTLA-4 and PD-1 blockades
Authors: Zhang Jing , Liu Dan , Liu Jiale , Han Yanfeng , Xu Haiyan , Leng Xigang , Kong Deling , Liu Lanxia
Published: July 2020

hSNPs elicit robust anti-tumor immune responses through targeted evading the immune suppressive function of Tregs and TIM3+ exhausted-like CD8+ T cells while promoting CD4+ T cells, especially ICOS+ Th1-like CD4+ T cells, and CD8+ effector T cells in a nonredundant fashion.
A novel targeted delivery system for drug-resistant hepatocellular carcinoma therapy
Authors: Xiao Li , Hou Yang , He Huimin , Cheng Sinan , Hou Yifan , Jin Huijuan , Song Xigui , Nie Guochao , Hou Yingchun
Published: July 2020

HCSP4-Lipo-DOX-miR101 is a novel and efficient approach for HCC targeted therapy with MDR inhibition in vitro and in vivo.
A stimulated liquid–gas phase transition nanoprobe dedicated to enhance the microwave thermoacoustic imaging contrast of breast tumors
Authors: Zhang Le , Qin Huan , Zeng Fanchu , Wu Zhujun , Wu Linghua , Zhao Shuxiang , Xing Da
Published: July 2020

Microwave-induced thermoacoustic imaging (MTAI), combining the advantages of the high contrast of microwave imaging and the high resolution of ultrasonic imaging, is a potential candidate for breast tumor detection.