bluuebunny/crossref_metadata_2025 huggingface dataset:
Synthesis of Low-Cost, Bio-Based Novel Adsorbent Material Using Charge-Transfer Interaction for Water Treatment from Several Pollutants: Waste to Worth
Authors: Almehizia Abdulrahman A. , Al-Omar Mohamed A. , Naglah Ahmed M. , Alkahtani Hamad M. , Obaidullah Ahmad J. , Bhat Mashooq A.
Published: April 2023

Tea is the third most consumed beverage in Saudi Arabia (a country in the Middle East) after water and Arabian coffee. Hence, a large amount of consumed tea leaves is discarded as solid waste. Waste tea leaves (WTLs) have no commercial value and could be considered as an environmentally sustainable costless material. This work aimed to manufacture an adsorbent material from the discarded WTLs and charge-transfer (CT) interaction and use this adsorbent material effectively for the removal of different kinds of pollutants from water. The adsorbent material was manufactured in three steps. First, a CrFeO3 metal composite was synthesized from the CT interaction between FeCl3 and CrCl3 with urea. Second, activated carbons were prepared from consumed WTLs using facile and clean treatments of pre-carbonization, and a simple potassium hydroxide (KOH) activation treatment. Finally, the adsorbent material was fabricated by grounding CrFeO3 composite with the activated carbons in a 1:10 molar ratio (metal composite to activated carbons). The prepared materials were characterized spectroscopically and morphologically using FT-IR, XRD, SEM/EDX, and TEM analysis. The synthesized absorbent material was used to adsorb two organic dyes (Azocarmine G2; M1, and Methyl violet 2B; M2), and two commercial pesticides (Tiller 480SL; M3, and Acochem 25% WP; M4) from aqueous solution, and it showed promising adsorption efficacy. The minimum adsorbent material’s dosage to obtain a maximum removal efficiency (R%) for M1, M2, M3, and M4 removal from 100 mL solution (100 mg/L) was 0.11, 0.14, 0.13, and 0.12 g, respectively. The max R% for M1 (96.8%) was achieved in the first 45 min, the max R% for M2, 95.5%, was achieved during the first 55 min, and the max R% for M3 (96.4%) was achieved in the first 35 min, while the max R% for M4, 98.6%, was achieved during the first 35 min.
Highly Reliable Temperature Sensor Based on p-GaN/AlGaN/GaN Hybrid Anode Diode with Wide Operation Temperature from 73 K to 573 K
Authors: Yang An , Wei Xing , Shen Wenchao , Hu Yu , Chen Tiwei , Wang Heng , Zhou Jiaan , Xing Runxian , Zhang Xiaodong , Yu Guohao , Fan Yaming , Cai Yong , Zeng Zhongming , Zhang Baoshun
Published: April 2023

A high-performance temperature sensor based on a p-GaN/AlGaN/GaN hybrid anode diode (HPT-HAD) fabricated by hydrogen plasma treatment is demonstrated. The sensor exhibits accurate and stable temperature responses from 73 to 573 K. The forward anode voltage is linearly proportional to the temperature over the measured temperature range at a fixed current. At a forward current density of 10−7 mA/mm, the device achieves a maximum sensitivity of 1.93 mV/K. The long-time anode current stress measurement reveals that the HPT-HAD shows almost no degradation even at 573 K for 1 h at a current of 100 μA, and the anode voltage shifts only 120 mV at 573 K for 1000 s at 1 nA. This work shows that the HPT-HAD temperature sensor can be reliably operated over a wide temperature range from cryogenic to high temperatures, so can be used in a variety of extreme environments.
Histological and Genetic Diversity in Ovarian Mucinous Carcinomas: A Pilot Study
Authors: Razia Sultana , Nakayama Kentaro , Yamashita Hitomi , Ishibashi Tomoka , Ishikawa Masako , Kanno Kosuke , Sato Seiya , Kyo Satoru
Published: April 2023

Tumor heterogeneity remains an ongoing challenge in the field of cancer therapy. Intratumor heterogeneity significantly complicates the diagnosis of cancer and presents challenging clinical problems due to resistance to drug therapy. This study aimed to elucidate the genetic changes histologically (mucinous cystadenoma (MCA), mucinous borderline tumor (MBT), and mucinous ovarian carcinoma (MOC)) in a portion of mucinous ovarian tumors within the same sample. Seven tumor samples obtained from different patients were used to evaluate the genetic mutations in each component. Intratumor genetic heterogeneity was observed in all patients; among them, BRAF (V600E) and p53 (T118I, P142S, T150I, and T170M) point mutations were observed in the MBT component, while KRAS (G12D and G13D) and PIK3CA (E545K) mutations were found in the MOC component. The current findings suggest that diverse genetic alterations occur in mucinous tumors, according to tumor histology. Tumor heterogeneity and genetic diversity in mucinous ovarian tumors might be the cause of treatment failure. Knowledge of intertumor heterogeneity may lead to an increased understanding of the tumor response to treatment.
Vegetation Structure and Invertebrate Food Availability for Birds in Intensively Used Arable Fields: Evaluation of Three Widespread Crops
Authors: Hološková Adriana , Kadlec Tomáš , Reif Jiří
Published: April 2023

Arable land covers a large part of the European landscape, and its biodiversity is declining rapidly due to agricultural intensification. Among the most threatened groups of organisms are insectivorous ground-foraging farmland birds. To reverse their decline, it is necessary to understand the impact of agriculture on the factors potentially shaping their populations. This study therefore evaluates the invertebrate food availability and vegetation structure of three widespread crops—wheat, maize and rapeseed—during their breeding. This research took place in Slovakia, the country with the largest average field size in the EU. Wheat overlapped bird habitat preferences for the longest part of their breeding season by vegetation structure, but it also had the most limited food supply due to frequent treatment with insecticides. Maize and rapeseed provided higher invertebrate abundance and biomass, but their stands created unsuitable vegetation structures for farmland bird nesting and foraging over a major part of the breeding season. The food supply improved closer to the field edges, but the birds’ ability to use these benefits could be considerably limited by the large field sizes. Therefore, conservation measures should include reduction of field size and insecticide application to improve the food supply and switch to spring sowing to improve the vegetation structure.
Hybrid Control and Energy Management of a Residential System Integrating Vehicle-to-Home Technology
Authors: El Harouri Khadija , El Hani Soumia , Naseri Nisrine , Elbouchikhi Elhoussin , Benbouzid Mohamed , Skander-Mustapha Sondes
Published: April 2023

Electric vehicles (EV) and photovoltaic (PV) systems are increasingly becoming environmentally friendly and more affordable solutions for consumers. This article discusses the integration of PV and EV in a residential system to meet the requirements of residential loads taking into account the PV supplied power, availability and the state of charge (SOC) of EVs. A hybrid control model has been proposed to control the residential system. The combined PI-Fuzzy logic controller is employed to control the buck-boost bi-directional converter. The DC-AC grid-side converter is controlled by the ADRC controller. The effectiveness of PI-Fuzzy logic controller in reducing voltage and current ripples and ADRC controller in rejecting disturbances is demonstrated in each case. A rule-based energy management strategy has been proposed to control the flow of energy between the components of the residential system. The suggested energy management system (EMS) covers every scenario that might occur. Whether the EV is linked to the home or not, and also takes into account the owner using the EV in an emergency situation. The EV operates in two modes, Home-to-Vehicle (H2V) mode and Vehicle-to-Home (V2H) mode, depending on the power produced by the PV and the conditions related to the EV. All possible scenarios are tested and validated. The simulation results show that the proposed EMS is a reliable solution that can reduce the power grid intervention.
Multi-Modal Fake News Detection via Bridging the Gap between Modals
Authors: Liu Peng , Qian Wenhua , Xu Dan , Ren Bingling , Cao Jinde
Published: April 2023

Multi-modal fake news detection aims to identify fake information through text and corresponding images. The current methods purely combine images and text scenarios by a vanilla attention module but there exists a semantic gap between different scenarios. To address this issue, we introduce an image caption-based method to enhance the model’s ability to capture semantic information from images. Formally, we integrate image description information into the text to bridge the semantic gap between text and images. Moreover, to optimize image utilization and enhance the semantic interaction between images and text, we combine global and object features from the images for the final representation. Finally, we leverage a transformer to fuse the above multi-modal content. We carried out extensive experiments on two publicly available datasets, and the results show that our proposed method significantly improves performance compared to other existing methods.
Online/Offline MA-CP-ABE with Cryptographic Reverse Firewalls for IoT
Authors: Li Juyan , Fan Ye , Bian Xuefen , Yuan Qi
Published: April 2023

Devices in the Internet of Things (IoT) usually use cloud storage and cloud computing to save storage and computing cost. Therefore, the efficient realization of one-to-many communication of data on the premise of ensuring the security of cloud storage data is a challenge. Ciphertext-Policy Attribute-Based Encryption (CP-ABE) can not only protect the security of data in the cloud and achieve one-to-many communication but also achieve fine-grained access control for data. However, the single-authority CP-ABE faces the crisis of single point of failure. In order to improve security, the Multi-Authority CP-ABE (MA-CP-ABE) is adopted. Although there are provably-secure MA-CP-ABE schemes, Edward Snowden’s research shows that provably-secure cryptographic schemes are vulnerable to backdoor attacks, resulting in secret disclosure, and thus threatening security. In addition, ABE requires huge computational overhead in key generation, encryption and decryption, which increase with the increase in the number of attributes and the complexity of the access structure, and there are a large number of resource-constrained devices in the IoT. To mitigate this issue, we construct the Online/Offline MA-CP-ABE with Cryptographic Reverse Firewalls (OO-MA-CP-ABE-CRFs) scheme. This scheme not only uses Cryptographic Reverse Firewall (CRF) to resist backdoor attacks but also uses online/offline key generation, online/offline encryption and outsourcing encryption technology to optimize the efficiency of the MA-CP-ABE scheme with reverse firewall, reducing the storage and computing cost of users. Finally, the security of the OO-MA-CP-ABE-CRFs scheme is proved, and the experimental results indicate that the scheme is efficient and practical.
Assessing Energy Performance Certificates for Buildings: A Fuzzy Set Qualitative Comparative Analysis (fsQCA) of Portuguese Municipalities
Authors: Koengkan Matheus , Silva Nuno , Fuinhas José Alberto
Published: April 2023

This article presents causal recipes leading to high and low energy consumption efficiency performances using fuzzy set Qualitative Comparative Analysis (fsQCA). The study found that several causal conditions are sufficient for high energy efficiency performance, including several fiscal and financial incentive policies, a highly educated population, many completed dwellings, and low GDP. The study also found that high inequality in completed dwellings and completed reconstructions, coupled with a low GDP and a low number of policies, lead to high energy consumption efficiency performance. In addition, the analysis showed slight differences between the yearly consistencies, suggesting that time effects are not a concern. On the other hand, a low education level, Gini coefficient, few completed dwellings and reconstructions, coupled with a low number of fiscal and financial policies, are the causal conditions leading to low energy consumption efficiency performance. The study’s results suggest that policymakers and stakeholders should consider a combination of several causal conditions when implementing energy efficiency policies. The study also highlights the need for policies focusing on education, fiscal and financial incentives, completed dwellings, and reconstructions to achieve high energy efficiency performance.
Thermomechanical and Thermofluid-Dynamic Coupled Analysis of the Top Cap Region of the Water-Cooled Lithium Lead Breeding Blanket for the EU DEMO Fusion Reactor
Authors: Gioè Alberto , Bongiovì Gaetano , Catanzaro Ilenia , Chiovaro Pierluigi , Di Maio Pietro Alessandro , Giambrone Salvatore , Quartararo Andrea , Vallone Eugenio , Arena Pietro , Basile Salvatore
Published: April 2023

In the EU, the Water-Cooled Lithium Lead (WCLL) Breeding Blanket (BB) concept is one of the candidates for the design of the DEMO reactor. From the past campaign of analysis emerged that the thermal-induced stress led to the failure in the verification of the RCC-MRx structural criteria. Hence, in this paper the classic conceptual design approach, based on a pure FEM thermal and structural analysis, is compared to a coupled thermofluid-dynamic/structural one. Even though the coupled approach requires tremendous modelling effort and computational burden, it surely allows determining the thermal field with a higher level of detail than the FEM analysis. Therefore, in this work, the focus is put on the impact of a more detailed thermal field on the DEMO WCLL BB global structural performances, focusing on the Top Cap region of its Central Outboard Blanket segment. The obtained results have allowed confirming the soundness of the design solution of the Top Cap region, except for concerns arising on the mass flow rate distribution. Moreover, results have shown that, globally, the pure FEM approach allows for obtaining more conservative results than the coupled one. This is a positive outcome in sight of the follow-up of the DEMO WCLL BB design, as it will be still possible adopting the pure FEM approach to quickly down-select design alternatives, using the most onerous coupled approach to finalise the most promising.
A Bilevel Stochastic Optimization Framework for Market-Oriented Transmission Expansion Planning Considering Market Power
Authors: Alnowibet Khalid A. , Alshamrani Ahmad M. , Alrasheedi Adel F.
Published: April 2023

Market power, defined as the ability to raise prices above competitive levels profitably, continues to be a prime concern in the restructured electricity markets. Market power must be mitigated to improve market performance and avoid inefficient generation investment, price volatility, and overpayment in power systems. For this reason, involving market power in the transmission expansion planning (TEP) problem is essential for ensuring the efficient operation of the electricity markets. In this regard, a methodological bilevel stochastic framework for the TEP problem that explicitly includes the market power indices in the upper level is proposed, aiming to restrict the potential market power execution. A mixed-integer linear/quadratic programming (MILP/MIQP) reformulation of the stochastic bilevel model is constructed utilizing Karush−Kuhn−Tucker (KKT) conditions. Wind power and electricity demand uncertainty are incorporated using scenario-based two-stage stochastic programming. The model enables the planner to make a trade-off between the market power indices and the investment cost. Using comparable results of the IEEE 118-bus system, we show that the proposed TEP outperforms the existing models in terms of market power indices and facilitates open access to the transmission network for all market participants.
Definition of Key Performance Indicators for Noise Monitoring Networks
Authors: Bolognese Matteo , Carpita Stefano , Fredianelli Luca , Licitra Gaetano
Published: April 2023

The European Noise Directive fixed a well-defined schedule on a five-year basis for the noise exposure evaluation of citizens. Noise monitoring is needed to validate noise maps of the main transportation infrastructures and it can also represent an improvement. By comparing with average noise maps, a properly set sensor network can report noise information in a more case-specific way, including time variations. To the best of the authors’ knowledge, key performance indicators (KPIs) evaluating the technical performance and financial sustainability of acoustic networks have never been presented in the scientific literature. Therefore, in the present work, a set of KPIs are proposed on the basis of KPIs defined for monitoring networks of other pollutants. It is then tuned according to noise monitoring, in line with the current legislations. As a case study, the performance of three monitoring networks installed in ports are evaluated and discussed. The resulting investment and maintenance costs can be helpful for decision makers to calculate the cost–benefits in the installation of new monitoring stations and also to evaluate the performance of the already-existing ones.
Flame Resistance and Bonding Performance of Plywood Fabricated by Guanidine Phosphate-Impregnated Veneers
Authors: Yan Yutao , Wang Jinhui , Shen Zhou , Bi Haiming , Shentu Baoqing
Published: April 2023

In this study, fire-retardant plywood was fabricated using a simple guanidine phosphate (GP) impregnation treatment of the veneers, and the influence of the treatment on the flame resistance and bonding strength of the plywood was fully investigated. The results showed that GP modification could effectively endow the plywood with excellent fire resistance and smoke-suppression effect. When 10% GP solution was applied, the limiting oxygen index (LOI) of the impregnated wood was up to 37%, which was almost twice of unmodified plywood. The heat-release rate (HRR) and total smoke production (TSP) were also greatly decreased from the pristine 94.14 MJ/m2 and 0.77 m2 to that of modified 43.94 MJ/m2 and 0.08 m2, respectively. The excellent fireproof performance was mainly due to the thermal decomposition of GP to phosphoric acid and guanidine during combustion, which could promote the catalytic carbonization of wood and release of incombustible CO2 and NH3 to dilute and decrease the combustible gases, thus collectively preventing the wood form burning. However, the guanidine phosphate modification could seriously damage the bond performance of plywood, especially the UF resin adhesive-bonded plywood. When 10% guanidine phosphate was applied, the dry and wet bonding strength of the UF resin adhesive-bonded plywood were decreased to only 0.7 MPa and 0.12 MPa, respectively, which may be due to the blocking effect of GP in wood pores and the hygroscopic and soluble properties of GP itself in water, thus decreasing the effective bonding between wood veneers. What’s worse, the poor water resistance of the UF resin adhesive was also adverse to the bonding strength of plywood. Surprisingly, the PF resin adhesive was proved to be suitable for gluing the GP-modified wood without obviously decreasing the bonding strength, which could be used to prepare plywood with both high bonding strength and flame resistance.
Fossils—A New Open Access Journal
Authors: Buffetaut Eric
Published: April 2023

As the British author H [...]
Identification of GLI1 and KIAA0825 Variants in Two Families with Postaxial Polydactyly
Authors: Ahmad Safeer , Ali Muhammad Zeeshan , Muzammal Muhammad , Khan Amjad Ullah , Ikram Muhammad , Muurinen Mari , Hussain Shabir , Loid Petra , Khan Muzammil Ahmad , Mäkitie Outi
Published: April 2023

Polydactyly is a rare autosomal dominant or recessive appendicular patterning defect of the hands and feet, phenotypically characterized by the duplication of digits. Postaxial polydactyly (PAP) is the most common form and includes two main types: PAP type A (PAPA) and PAP type B (PAPB). Type A involves a well-established extra digit articulated with the fifth or sixth metacarpal, while type B presents a rudimentary or poorly developed superfluous digit. Pathogenic variants in several genes have been identified in isolated and syndromic forms of polydactyly. The current study presents two Pakistani families with autosomal recessive PAPA with intra- and inter-familial phenotype variability. Whole-exome sequencing and Sanger analysis revealed a novel missense variant in KIAA0825 (c.3572C>T: p.Pro1191Leu) in family A and a known nonsense variant in GLI1 (c.337C>T: p.Arg113*) in family B. In silico studies of mutant KIAA0825 and GLI1 proteins revealed considerable structural and interactional modifications that suggest an abnormal function of the proteins leading to the disease phenotype. The present study broadens the mutational spectrum of KIAA0825 and demonstrates the second case of a previously identified GLI1 variant with variable phenotypes. These findings facilitate genetic counseling in Pakistani families with a polydactyly-related phenotype.
High-Resolution Imaging Methods for Identification of Calcium Crystal Types in Osteoarthritis
Authors: Buchholz Adrian , Stücker Sina , Koßlowski Franziska , Lohmann Christoph H. , Bertrand Jessica
Published: April 2023

The detection and differentiation of BCP and CPP crystals in calcified tissue is an important factor in the context of research and potential future treatment of osteoarthritis and chondrocalcinosis. Current standard methods originate from clinical practice and often lack precision in the correct identification of the calcium crystal type. In this work, a step-by-step guide for the use of the high-resolution imaging methods of tissue sections, Raman spectroscopy and scanning electron microscopy (SEM) in combination with energy-dispersive X-ray spectroscopy (EDS), for calcium crystal identification is presented. Sample preparation including Von Kossa staining, measurement and measurement parameters, data processing and data analysis methods are discussed and described. Furthermore, the different methods are compared to show advantages and disadvantages. Overall, Raman spectroscopy is a reasonable method from an economic point of view and regarding the time/effort required for acquiring highly reliable data in calcium crystal identification. Potentially, semi-quantitative results can be obtained with little effort and without the destruction of the respective test sample. The analysis/penetration depth during the Raman measurements, which is not precisely defined, poses a potential problem for accuracy. SEM can also be used for this task but requires more time, advanced technical knowledge and a pre-treatment of the samples using, e.g., gold sputtering, which may distort further analysis on the specific specimen. Therefore, this technique yields additional value compared to Raman spectroscopy only with additional research questions needed to be answered in the same sample, such as analysis of the sample topography or analysis of other unknown particles/deposits using EDS. The methods described in this manuscript are helpful for retrospective analyses in the context of research, but can also be used for potential future treatment strategies to discriminate between osteoarthritis and chondrocalcinosis patients.
Multianalytical Non-Invasive Characterization of ‘Mater Boni Consilii’ Iconography Oil Painting
Authors: Valentini Federica , De Angelis Sara , Marinelli Livia , Zaratti Camilla , Colapietro Marcello , Tarquini Ombretta , Macchia Andrea
Published: April 2023

This paper presents the results of non-invasive diagnostic investigations performed on the canvas oil painting depicting the Marian iconography “Mater Boni Consilii”. The painting, whose author and origin are unknown, was found in an old shop in Florence following the overflowing of the Arno River in 1966. In order to define the importance of the artwork, a multianalytical analysis was performed on the painting, using multispectral imaging, X-ray fluorescence (XRF), and Fourier Transform Infrared Spectroscopy (FTIR-ATR) for the definition of materials, with a particular focus on the identification of pigments. The results allowed for the drawing up of a color palette, composed mainly of ochre and earth pigments, cinnabar, lithopone, lead white, and ultramarine pigments. After cross-referencing the acquired information with other findings, it was possible to place the painting in the period between the end of the XIXth and the beginning of the XXth centuries.
The Potential of Aerial Reconnaissance in the Detection, Mapping and 3D Reconstruction Modelling of Crop-Marked Military Components of Bohemia’s Postmedieval and Early Industrial Landscape
Authors: Gojda Martin
Published: April 2023

From the 17th to the 19th century, a score of military events, campaigns and battles took place in the Czech lands, leaving numerous traces and distinctly changing the appearance of the cultural landscape in some regions. The results of long-term aerial-archeological surveys in the Czech lands have demonstrated that this detection method is advantageous in identifying buried sites built in the past in the context of military conflicts. Experience hitherto has made it possible to label archeological remote sensing as a collection of the potentially most effective methods for uncovering sites of field fortifications dated to the modern period and the beginning of the industrial era. This includes finds of both solitary sites and segments of strategically built fortification lines. This paper is an attempt to critically evaluate these hitherto recorded landmarks which have been discovered and documented via aerial prospection from the 1990s to the present. At the same time, this study reflects on the possibilities offered by the modern methods of remote sensing which have played a significant role in the discovery, mapping, documentation, digital terrain modelling, and the 3D virtual reconstructions of these sites.
A Place-Based Sustainability Approach to Learning about Photovoltaic Solar Energy
Authors: Forbes Marissa H. , Lord Susan M.
Published: April 2023

An ethical and effective engineering practice is inherently place-responsive and designs for a sustainable future. Engineering students must therefore be educated within a sustainable and sociotechnical paradigm. In the spring of 2022, the integrated engineering department of the University of San Diego offered a new photovoltaic solar energy course for junior and senior students. Informed by place-based pedagogies and culturally sustaining pedagogies, we designed the course to be relevant to the students’ lived experiences by coupling the learning about the technical elements of solar energy with a focus on solar energy projects and sustainability on campus. Prior to running the course, we identified four potential new solar investment/upgrade projects for the university. We divided the class into four teams, with each team assigned to assess the feasibility of a solar project through (1) social, (2) technical, (3) economic, and (4) environmental analyses. Finally, the students integrated their findings and made recommendations to key university stakeholders about how to proceed with solar energy investments on campus. In this paper, we share the course project design, our findings from implementing it, and ideas for adapting it to other place-based sustainability learning experiences.
The LibHLH22 and LibHLH63 from Lilium ‘Siberia’ Can Positively Regulate Volatile Terpenoid Biosynthesis
Authors: Feng Yuan , Guo Ziyu , Zhong Jian , Liang Yilin , Zhang Peng , Sun Ming
Published: April 2023

Basic helix-loop-helix (bHLH) transcription factors (TFs) play irreplaceable roles in plant growth and development, especially in plant secondary metabolism. However, the functions of most bHLH TFs in Lilium ‘Siberia’ are still unknown, especially their roles in regulating floral fragrance. In this study, two bHLH TFs in lily, i.e., LibHLH22 and LibHLH63, were identified and functionally characterized. A bioinformatics analysis revealed that LibHLH22 and LibHLH63 were unstable proteins. Subcellular localization demonstrated that LibHLH22 and LibHLH63 proteins were in the cell nucleus. Quantitative real-time PCR showed that the highest expression level of LibHLH22 was at the initial flowering stage and in the stigma, and the highest expression level of LibHLH63 was at the budding stage and in the filaments. The results of transient overexpression and virus-induced gene silencing (VIGS) of LibHLH22 and LibHLH63 in lily petals showed that these two transcription factors significantly promoted the expression of LiDXR and LiTPS2, and thus, markedly enhanced the release of floral fragrance. Our results indicated that LibHLH22 and LibHLH63 could effectively regulate the fragrance of Lilium ‘Siberia’, laying the foundation for fragrance breeding and improving the terpenoid transcriptional regulatory pathway.
Characterization of Arsenic and Atrazine Contaminations in Drinking Water in Iowa: A Public Health Concern
Authors: Roh Taehyun , Knappett Peter S. K. , Han Daikwon , Ludewig Gabriele , Kelly Kevin M. , Wang Kai , Weyer Peter J.
Published: April 2023

Arsenic and atrazine are two water contaminants of high public health concern in Iowa. The occurrence of arsenic and atrazine in drinking water from Iowa’s private wells and public water systems was investigated over several decades. In this study, the percentages of detection and violation of regulations were compared over region, season, and water source, and factors affecting the detection and concentration of arsenic and atrazine were analyzed using a mixed-effects model. Atrazine contamination in drinking water was found to vary by region, depending on agricultural usage patterns and hydrogeological features. The annual median atrazine levels of all public water systems were below the drinking water standard of 3 ppb in 2001–2014. Around 40% of public water systems contained arsenic at levels > 1 ppb in 2014, with 13.8% containing arsenic at levels of 5–10 ppb and 2.6% exceeding 10 ppb. This unexpected result highlights the ongoing public health threat posed by arsenic in drinking water in Iowa, emphasizing the need for continued monitoring and mitigation efforts to reduce exposure and associated health risks. Additionally, an atrazine metabolite, desethylatrazine, should be monitored to obtain a complete account of atrazine exposure and possible health effects.
High-Dose Ambroxol Therapy in Type 1 Gaucher Disease Focusing on Patients with Poor Response to Enzyme Replacement Therapy or Substrate Reduction Therapy
Authors: Istaiti Majdolen , Frydman Dafna , Dinur Tama , Szer Jeff , Revel-Vilk Shoshana , Zimran Ari
Published: April 2023

Ambroxol hydrochloride (ABX), an oral mucolytic drug available over the counter for many years, acts as a pharmacological chaperone for mutant glucocerebrosidase, albeit at higher doses. Proof-of-concept reports have been published over the past decade on all three types of Gaucher disease (GD). Here, we assess the safety and efficacy of 12 months of 600 mg ambroxol per day in three groups of Type 1 GD patients with a suboptimal response to enzyme replacement therapy (ERT) or substrate reduction therapy (SRT), defined as platelet count < 100 × 103/L, lumbar spine bone density T-score < −2.0, and/or LysoGb1 > 200 ng/mL, and for a group of naïve patients who had abnormal values in two of these three parameters. We enrolled 40 patients: 28 ERT- or SRT-treated, and 12 naïve. There were no severe adverse effects (AEs). There were 24 dropouts, mostly due to AEs (n = 12), all transient, and COVID-19 (n = 7). Among the 16 completers, 5 (31.2%) had a >20% increase in platelet count, 6 (37.5%) had a >0.2 increase in T-score, and 3 (18.7%) had a >20% decrease in Lyso-Gb1. This study expands the number of patients exposed to high-dose ABX, showing good safety and satisfactory efficacy, and provides an additional rationale for adding off-label ABX to the arsenal of therapies that could be offered to patients with GD1 and a suboptimal response or those unable to receive ERT or SRT.
Identification of Optimal Reference Genes for qRT-PCR Normalization for Physical Activity Intervention and Omega-3 Fatty Acids Supplementation in Humans
Authors: Grzybkowska Agata , Anczykowska Katarzyna , Antosiewicz Jędrzej , Olszewski Szczepan , Dzitkowska-Zabielska Magdalena , Tomczyk Maja
Published: April 2023

The quantitative polymerase chain reaction (qRT-PCR) technique gives promising opportunities to detect and quantify RNA targets and is commonly used in many research fields. This study aimed to identify suitable reference genes for physical exercise and omega-3 fatty acids supplementation intervention. Forty healthy, physically active men were exposed to a 12-week eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) supplementation and standardized endurance training protocol. Blood samples were collected before and after the intervention and mRNA levels of six potential reference genes were tested in the leukocytes of 18 eligible participants using the qRT-PCR method: GAPDH (Glyceraldehyde-3-phosphate dehydrogenase), ACTB (Beta actin), TUBB (Tubulin Beta Class I), RPS18 (Ribosomal Protein S18), UBE2D2 (Ubiquitin-conjugating enzyme E2 D2), and HPRT1 (Hypoxanthine Phosphoribosyltransferase 1). The raw quantification cycle (Cq) values were then analyzed using RefFinder, an online tool that incorporates four different algorithms: NormFinder, geNorm, BestKeeper, and the comparative delta-Ct method. Delta-Ct, NormFinder, BestKeeper, and RefFinder comprehensive ranking have found GAPDH to be the most stably expressed gene. geNorm has identified TUBB and HPRT as the most stable genes. All algorithms have found ACTB to be the least stably expressed gene. A combination of the three most stably expressed genes, namely GAPDH, TUBB, and HPRT, is suggested for obtaining the most reliable results.
A Dual-Function “TRE-Lox” System for Genetic Deletion or Reversible, Titratable, and Near-Complete Downregulation of Cathepsin D
Authors: Terron Heather M. , Maranan Derek S. , Burgard Luke A. , LaFerla Frank M. , Lane Shelley , Leissring Malcolm A.
Published: April 2023

Commonly employed methods for reversibly disrupting gene expression, such as those based on RNAi or CRISPRi, are rarely capable of achieving >80–90% downregulation, making them unsuitable for targeting genes that require more complete disruption to elicit a phenotype. Genetic deletion, on the other hand, while enabling complete disruption of target genes, often produces undesirable irreversible consequences such as cytotoxicity or cell death. Here we describe the design, development, and detailed characterization of a dual-function “TRE-Lox” system for effecting either (a) doxycycline (Dox)-mediated downregulation or (b) genetic deletion of a target gene—the lysosomal aspartyl protease cathepsin D (CatD)—based on targeted insertion of a tetracycline-response element (TRE) and two LoxP sites into the 5′ end of the endogenous CatD gene (CTSD). Using an optimized reverse-tetracycline transrepressor (rtTR) variant fused with the Krüppel-associated box (KRAB) domain, we show that CatD expression can be disrupted by as much as 98% in mouse embryonic fibroblasts (MEFs). This system is highly sensitive to Dox (IC50 = 1.46 ng/mL) and results in rapid (t1/2 = 0.57 d) and titratable downregulation of CatD. Notably, even near-total disruption of CatD expression was completely reversed by withdrawal of Dox. As expected, transient expression of Cre recombinase results in complete deletion of the CTSD gene. The dual functionality of this novel system will facilitate future studies of the involvement of CatD in various diseases, particularly those attributable to partial loss of CatD function. In addition, the TRE-Lox approach should be applicable to the regulation of other target genes requiring more complete disruption than can be achieved by traditional methods.
Insights into the Effects of Insecticides on Aphids (Hemiptera: Aphididae): Resistance Mechanisms and Molecular Basis
Authors: Kaleem Ullah Rana Muhammad , Gao Fukun , Sikandar Aatika , Wu Haiyan
Published: April 2023

With the passage of time and indiscreet usage of insecticides on crops, aphids are becoming resistant to their effect. The different classes of insecticides, including organophosphates, carbamates, pyrethroids and neonicotinoids, have varied effects on insects. Furthermore, the molecular effects of these insecticides in aphids, including effects on the enzymatic machinery and gene mutation, are resulting in aphid resistance to the insecticides. In this review, we will discuss how aphids are affected by the overuse of pesticides, how resistance appears, and which mechanisms participate in the resistance mechanisms in various aphid species as significant crop pests. Gene expression studies were analyzed using the RNA-Seq technique. The stress-responsive genes were analyzed, and their expression in response to insecticide administration was determined. Putative insecticide resistance-related genes, cytochrome P450, glutathione S-transferase, carboxylesterase CarEs, ABC transporters, cuticle protein genes, and trypsin-related genes were studied. The review concluded that if insecticide-susceptible aphids interact with ample dosages of insecticides with sublethal effects, this will result in the upregulation of genes whose primary role is to detoxify insecticides. In the past decade, certain advancements have been observed regarding insecticide resistance on a molecular basis. Even so, not much is known about how aphids detoxify the insecticides at molecular level. Thus, to attain equilibrium, it is important to observe the manipulation of pest and insect species with the aim of restoring susceptibility to insecticides. For this purpose, this review has included critical insights into insecticide resistance in aphids.
Transient Changes in Serum CEA, CA19-9, CRP, YKL-40, and IL-6 during Adjuvant Chemotherapy and Survival of Patients with Colorectal Cancer
Authors: Lehtomäki Kaisa , Heervä Eetu , Kellokumpu-Lehtinen Pirkko-Liisa , Mustonen Harri , Salminen Tapio , Joensuu Heikki , Hermunen Kethe , Boisen Mogens Karsbøl , Johansen Julia Sidenius , Haglund Caj , Osterlund Pia
Published: April 2023

Serum carcinoembryonic antigen (CEA) is frequently monitored to detect colorectal cancer (CRC) recurrence after surgery. The clinical significance of transiently increased CEA during adjuvant chemotherapy is poorly understood. Serum CEA, CA19-9, CRP, YKL-40, and IL-6 were measured before, during, and after adjuvant 5-fluorouracil-based chemotherapy in the randomised LIPSYT study population. The biomarker kinetic patterns were classified into three groups: no increase, a transient increase (≥10% increase followed by a decrease), and a persistent increase during the adjuvant treatment, and the associations of these patterns with disease free-survival (DFS) and overall survival (OS) were investigated by using Cox regression analyses. The findings were validated in two single-centre cohorts that received modern adjuvant chemotherapy. A transient increase in CEA occurred in about a half of the patients during chemotherapy, in all the cohorts. The patients with a transient increase had a roughly similar DFS and OS to the patients with no increase, and a more favourable survival compared to the patients with a persistent increase. In the LIPSYT cohort, the hazard ratio was 0.21 for DFS (CI95% 0.07–0.66) and 0.24 for OS (CI95% 0.08–0.76). Transient increases in CA19-9 and YKL-40 tended to be associated with a favourable survival. A transient increase in CEA during adjuvant chemotherapy is associated with a favourable survival when compared with a persistent increase.
Evidence Linking PPARG Genetic Variants with Periodontitis and Type 2 Diabetes Mellitus in a Brazilian Population
Authors: Cirelli Thamiris , Nicchio Ingra G. , Bussaneli Diego G. , Silva Bárbara R. , Nepomuceno Rafael , Orrico Silvana R. P. , Cirelli Joni A. , Theodoro Letícia H. , Barros Silvana P. , Scarel-Caminaga Raquel M.
Published: April 2023

The peroxisome proliferator-activated receptor gamma (PPARG) gene encodes a transcription factor involved in the regulation of complex metabolic and inflammatory diseases. We investigated whether single nucleotide polymorphisms (SNPs) and haplotypes of the PPARG gene could contribute with susceptibility to develop periodontitis alone or together with type 2 diabetes mellitus (T2DM). Moreover, we evaluated the gene–phenotype association by assessing the subjects’ biochemical and periodontal parameters, and the expression of PPARG and other immune response–related genes. We examined 345 subjects with a healthy periodontium and without T2DM, 349 subjects with moderate or severe periodontitis but without T2DM, and 202 subjects with moderate or severe periodontitis and T2DM. PPARG SNPs rs12495364, rs1801282, rs1373640, and rs1151999 were investigated. Multiple logistic regressions adjusted for age, sex, and smoking status showed that individuals carrying rs1151999-GG had a 64% lower chance of developing periodontitis together with T2DM. The CCGT haplotype increased the risk of developing periodontitis together with T2DM. The rs1151999-GG and rs12495364-TC were associated with reduced risk of obesity, periodontitis, elevated triglycerides, and elevated glycated hemoglobin, but there was no association with gene expression. Polymorphisms of the PPARG gene were associated with developing periodontitis together with T2DM, and with obesity, lipid, glycemic, and periodontal characteristics.
WAX INDUCER 1 Regulates β-Diketone Biosynthesis by Mediating Expression of the Cer-cqu Gene Cluster in Barley
Authors: Gerasimova Sophia V. , Kolosovskaya Ekaterina V. , Vikhorev Alexander V. , Korotkova Anna M. , Hertig Christian W. , Genaev Mikhail A. , Domrachev Dmitry V. , Morozov Sergey V. , Chernyak Elena I. , Shmakov Nikolay A. , Vasiliev Gennady V. , Kochetov Alex V. , Kumlehn Jochen , Khlestkina Elena K.
Published: April 2023

Plant surface properties are crucial determinants of resilience to abiotic and biotic stresses. The outer layer of the plant cuticle consists of chemically diverse epicuticular waxes. The WAX INDUCER1/SHINE subfamily of APETALA2/ETHYLENE RESPONSIVE FACTORS regulates cuticle properties in plants. In this study, four barley genes homologous to the Arabidopsis thaliana AtWIN1 gene were mutated using RNA-guided Cas9 endonuclease. Mutations in one of them, the HvWIN1 gene, caused a recessive glossy sheath phenotype associated with β-diketone deficiency. A complementation test for win1 knockout (KO) and cer-x mutants showed that Cer-X and WIN1 are allelic variants of the same genomic locus. A comparison of the transcriptome from leaf sheaths of win1 KO and wild-type plants revealed a specific and strong downregulation of a large gene cluster residing at the previously known Cer-cqu locus. Our findings allowed us to postulate that the WIN1 transcription factor in barley is a master mediator of the β-diketone biosynthesis pathway acting through developmental stage- and organ-specific transactivation of the Cer-cqu gene cluster.
Erucin, an H2S-Releasing Isothiocyanate, Exerts Anticancer Effects in Human Triple-Negative Breast Cancer Cells Triggering Autophagy-Dependent Apoptotic Cell Death
Authors: Bello Ivana , Smimmo Martina , d’Emmanuele di Villa Bianca Roberta , Bucci Mariarosaria , Cirino Giuseppe , Panza Elisabetta , Brancaleone Vincenzo
Published: April 2023

Breast cancer is the most frequent form of cancer occurring in women of any age. Among the different types, the triple-negative breast cancer (TNBC) subtype is recognized as the most severe form, being associated with the highest mortality rate. Currently, there are no effective treatments for TNBC. For this reason, the research of novel therapeutics is urgently needed. Natural products and their analogs have historically made a major contribution to pharmacotherapy and the treatment of various human diseases, including cancer. In this study, we explored the potential anti-cancer effects of erucin, the most abundant H2S-releasing isothiocyanate present in arugula (Eruca sativa) in MDA-MB-231 cells, a validated in vitro model of TNBC. We found that erucin, in a concentration-dependent manner, significantly inhibited MDA-MB-231 cell proliferation by inducing apoptosis and autophagy. Additionally, erucin prevented intracellular ROS generation promoting the expression of key antioxidant genes and halted MDA-MB-231 cell migration, invasion, and colony formation. In conclusion, using a cellular and molecular biology approach, we show that the consumption of erucin could represent a novel and promising strategy for intervention against TNBC.
LncRNA TCONS_00323213 Promotes Myogenic Differentiation by Interacting with PKNOX2 to Upregulate MyoG in Porcine Satellite Cells
Authors: Li Mengxun , Liu Quan , Xie Su , Fu Chong , Li Jiaxuan , Tian Cheng , Li Xin , Li Changchun
Published: April 2023

Myogenic differentiation is a complex biological process that is regulated by multiple factors, among which long noncoding RNAs (lncRNAs) play an essential role. However, in-depth studies on the regulatory mechanisms of long noncoding RNAs (lncRNAs) in myogenic differentiation are limited. In this study, we characterized the role of the novel lncRNA TCONS_00323213, which is upregulated during porcine skeletal muscle satellite cell (PSC) differentiation in myogenesis. We found that TCONS_00323213 affected the proliferation and differentiation of PSC in vitro. We performed quantitative polymerase chain reaction (qPCR), 5-ethynyl-20-deoxyuridine (EdU), western blotting, immunofluorescence staining, pull-down assays, and cleavage under targets and tagmentation (CUT and Tag) assays to clarify the effects and action mechanisms of TCONS_00323213. LncRNA TCONS_00323213 inhibited myoblast proliferation based on analyses of cell survival rates during PSC proliferation. Functional analyses revealed that TCONS_00323213 promotes cell differentiation and enhances myogenin (MyoG), myosin heavy chain (MyHC), and myocyte enhancer factor 2 (MEF2C) during myoblast differentiation. As determined by pull-down and RNA immunoprecipitation (RIP) assays, the lncRNA TCONS_00323213 interacted with PBX/Knotted Homeobox 2 (PKNOX2). CUT and Tag assays showed that PKNOX2 was significantly enriched on the MyoG promoter after lncRNA TCONS_00323213 knockdown. Our findings demonstrate that the interaction between lncRNA TCONS_00323213 and PKNOX2 relieves the inhibitory effect of PKNOX2 on the MyoG promoter, increases its expression, and promotes PSC differentiation. This novel role of lncRNA TCONS_00323213 sheds light on the molecular mechanisms by which lncRNAs regulate porcine myogenesis.
Checkpoint Kinase 1 Is a Key Signal Transducer of DNA Damage in the Early Mammalian Cleavage Embryo
Authors: Baran Vladimír , Mayer Alexandra
Published: April 2023

After fertilization, remodeling of the oocyte and sperm genome is essential for the successful initiation of mitotic activity in the fertilized oocyte and subsequent proliferative activity of the early embryo. Despite the fact that the molecular mechanisms of cell cycle control in early mammalian embryos are in principle comparable to those in somatic cells, there are differences resulting from the specific nature of the gene totipotency of the blastomeres of early cleavage embryos. In this review, we focus on the Chk1 kinase as a key transduction factor in monitoring the integrity of DNA molecules during early embryogenesis.