bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Dietary Crude Protein on Animal Performance, Blood Biochemistry Profile, Ruminal Fermentation Parameters and Carcass and Meat Quality of Heavy Fattening Assaf Lambs
Authors: Saro Cristina , Mateo Javier , Caro Irma , Carballo Diego Eloy , Fernández Miguel , Valdés Carmen , Bodas Raúl , Giráldez Francisco Javier
Published: November 2020

Thirty Assaf male lambs (30 ± 1.9 kg of body weight) were allocated to three groups fed diets differing in their crude protein (CP) contents (low protein (LP), 134 g CP/kg dry matter (DM); medium protein (MP), 157 g CP/kg DM; and high protein (HP), 173 g CP/kg DM) to test the effect of dietary protein content on animal performance, rumen function, animal health, and carcass and meat quality. Feed intake was recorded daily, and animals were weighed every second week. Lambs were blood-sampled to determine their acid–base status and biochemical profile. After 70 days of trial, lambs were slaughtered, and the ruminal content was collected to assess ruminal fermentation. Finally, carcass and meat quality were evaluated. Dry matter intake and average daily gain increased (p < 0.05) when increasing the level of dietary CP. There were not significant differences (p > 0.05) in the evaluated parameters in the rumen fluid of lambs. There were not significant differences in carcass or meat quality (p > 0.05) and in those parameters related to blood acid–base status. Several biochemical parameters showed differences depending on diet CP level (urea, protein, albumin, glucose, and calcium; p < 0.05). Feeding costs calculated in relation to cold carcass weight decreased when dietary CP decreased. The results suggested that a dietary protein content greater than 157 g/kg DM would be required to maximize growth performance in Assaf male fattening lambs under 50 kg of body weight. However, a protein content beyond that level was not found to improve either carcass or meat quality and could worsen profitability.
Streptococcus dysgalactiae—Contagious or Environmental?
Authors: Wente Nicole , Krömker Volker
Published: November 2020

Streptococcus dysgalactiae is among the most important pathogens causing bovine mastitis. Unfortunately, there is presently a lack of clear knowledge about the mode of transmission—contagious or environmental—of this pathogen. To obtain more information on this, knowledge of the genetic diversity of the isolated microorganisms at the farm level can be useful. To observe the strain variety in different herds of cattle, isolates of Strep. dysgalactiae were collected from clinical mastitis samples at different farms, and the strains were typed using the pulsed-field gel electrophoresis (PFGE) method. Overall, we performed strain typing on 93 isolates from 16 farms in Germany and used an index to describe the degree of contagiosity of Strep. dysgalactiae at each farm. This index (CI) represents the number of isolates divided by the number of strains found in mastitis milk of clinical cases within a period of 14 months. The results differed between the farms. In one farm, all six Strep. dysgalactiae cases that occurred during the study period were caused by a single strain (CI = 6), while in another farm the six cases that occurred were caused by five different strains (CI = 1.2). All other farms fell between these two extremes. This indicates that Strep. dysgalactiae infections can occur via several routes of transmission. At the farm level, strain comparisons are necessary to determine the routes of transmission. Two strains were able to survive on the farm for a minimum of 14 months.
Genome-Wide Association Analysis Identified BMPR1A as a Novel Candidate Gene Affecting the Number of Thoracic Vertebrae in a Large White × Minzhu Intercross Pig Population
Authors: Liu Qian , Yue Jingwei , Niu Naiqi , Liu Xin , Yan Hua , Zhao Fuping , Hou Xinhua , Gao Hongmei , Shi Lijun , Wang Lixian , Wang Ligang , Zhang Longchao
Published: November 2020

The number of vertebrae (NV), especially the number of thoracic vertebrae (NTV), varies among pig breeds. The NTV is controlled by vertebral segmentation and the number of somites during embryonic development. Although there is a high correlation between the NTV and NV, studies on a fixed NV have mainly considered the absolute numbers of thoracic vertebrae instead of vertebral segmentation. Therefore, this study aimed to discover variants associated with the NTV by considering the effect of the NV in pigs. The NTV and NV of 542 F2 individuals from a Large White × Minzhu pig crossbreed were recorded. All animals were genotyped for VRTN g.19034 A > C, LTBP2 c.4481A > C, and 37 missense or splice variants previously reported in a 951-kb interval on SSC7 and 147 single nucleotide polymorphisms (SNPs) on SSC14. To identify NTV-associated SNPs, we firstly performed a genome-wide association study (GWAS) using the Q + K (population structure + kinship matrix) model in TASSEL. With the NV as a covariate, the obtained data were used to identify the SNPs with the most significant genome-wide association with the NTV by performing a GWAS on a PorcineSNP60K Genotyping BeadChip. Finally, a conditional GWAS was performed by fixing this SNP. The GWAS showed that 31 SNPs on SSC7 have significant genome-wide associations with the NTV. No missense or splice variants were found to be associated with the NTV significantly. A linkage disequilibrium analysis suggested the existence of quantitative trait loci (QTL) in a 479-Kb region on SSC7, which contained a critical candidate gene FOS for the NTV in pigs. Subsequently, a conditional GWAS was performed by fixing M1GA0010658, the most significant of these SNPs. Two SNPs in BMPR1A were found to have significant genome-wide associations and a significant dominant effect. The leading SNP, S14_87859370, accounted for 3.86% of the phenotypic variance. Our study uncovered that regulation variants in FOS on SSC7 and in BMPR1A on SSC14 might play important roles in controlling the NTV, and thus these genetic factors may be harnessed for increasing the NTV in pigs.
Dog Welfare, Well-Being and Behavior: Considerations for Selection, Evaluation and Suitability for Animal-Assisted Therapy
Authors: Winkle Melissa , Johnson Amy , Mills Daniel
Published: November 2020

Health care and human service providers may include dogs in formal intervention settings to positively impact human physical, cognitive and psychosocial domains. Dogs working within this context are asked to cope with a multitude of variables including settings, populations, activities, and schedules. In this article, the authors highlight how both the preparation and operation of dogs within animal-assisted therapy (AAT) differs from less structured animal-assisted activities (AAA) and more exclusive assistance animal work; the authors highlight the gaps in our knowledge in this regard, and propose an ethically sound framework for pragmatic solutions. This framework also emphasizes the need for good dog welfare to safeguard all participants. If dogs are not properly matched to a job or handler, they may be subjected to unnecessary stress, anxiety, and miscommunication that can lead to disinterest in the work, overt problematic behavioral or health outcomes, or general unsuitability. Such issues can have catastrophic outcomes for the AAT. The authors propose standards for best practices for selection, humane-based preparation and training, and ongoing evaluation to ensure the health, welfare and well-being of dogs working in AAT, which will have concomitant benefits for clients and the professionalism of the field.
Can We Exploit β-Lactamases Intrinsic Dynamics for Designing More Effective Inhibitors?
Authors: Gianquinto Eleonora , Tondi Donatella , D'Arrigo Giulia , Lazzarato Loretta , Spyrakis Francesca
Published: November 2020

β-lactamases (BLs) represent the most frequent cause of antimicrobial resistance in Gram-negative bacteria. Despite the continuous efforts in the development of BL inhibitors (BLIs), new BLs able to hydrolyze the last developed antibiotics rapidly emerge. Moreover, the insurgence rate of effective mutations is far higher than the release of BLIs able to counteract them. This results in a shortage of antibiotics that is menacing the effective treating of infectious diseases. The situation is made even worse by the co-expression in bacteria of BLs with different mechanisms and hydrolysis spectra, and by the lack of inhibitors able to hit them all. Differently from other targets, BL flexibility has not been deeply exploited for drug design, possibly because of the small protein size, for their apparent rigidity and their high fold conservation. In this mini-review, we discuss the evidence for BL binding site dynamics being crucial for catalytic efficiency, mutation effect, and for the design of new inhibitors. Then, we report on identified allosteric sites in BLs and on possible allosteric inhibitors, as a strategy to overcome the frequent occurrence of mutations in BLs and the difficulty of competing efficaciously with substrates. Nevertheless, allosteric inhibitors could work synergistically with traditional inhibitors, increasing the chances of restoring bacterial susceptibility towards available antibiotics.
Peri-Implant Diseases: Diagnosis, Clinical, Histological, Microbiological Characteristics and Treatment Strategies. A Narrative Review
Authors: Kormas Ioannis , Pedercini Chantal , Pedercini Alessandro , Raptopoulos Michail , Alassy Hatem , Wolff Larry F.
Published: November 2020

Since the use of dental implants is continuously increasing, it is imperative for dental practitioners to understand the nature and treatment of peri-implant diseases. The purpose of this manuscript is to comprehensively review peri-implant diseases, their characteristics, as well as their non-surgical and surgical treatment. To that end, the current literature was searched and a narrative review was conducted. It is essential that the case definitions described in the 2017 World Workshop on the Classification of Periodontal and Peri-implant Diseases and Conditions are used to diagnose and classify peri-implant health, peri-implant mucositis and peri-implantitis. While recent epidemiologic studies on peri-implant diseases exist, there is great heterogeneity in the definition of these conditions. Several risk factors and indicators are reported in the literature, with smoking and diabetes being the most universally accepted. In peri-implant mucositis, non-surgical treatment seems to be sufficient. However, for the treatment of peri-implantitis, a surgical approach, which includes open-flap debridement, apically positioned flap and guided bone regeneration, is considered more appropriate. A great variety of adjuncts to mechanical treatment have been reported with controversial results. Finally, studies comparing results from different peri-implantitis treatments are warranted in randomized controlled clinical trials in order to provide stronger evidence-based approaches.
Inhibition of Campylobacter jejuni Biofilm Formation by D-Amino Acids
Authors: Elgamoudi Bassam A. , Taha Taha , Korolik Victoria
Published: November 2020

The ability of bacterial pathogens to form biofilms is an important virulence mechanism in relation to their pathogenesis and transmission. Biofilms play a crucial role in survival in unfavorable environmental conditions, acting as reservoirs of microbial contamination and antibiotic resistance. For intestinal pathogen Campylobacter jejuni, biofilms are considered to be a contributing factor in transmission through the food chain and currently, there are no known methods for intervention. Here, we present an unconventional approach to reducing biofilm formation by C. jejuni by the application of D-amino acids (DAs), and L-amino acids (LAs). We found that DAs and not LAs, except L-alanine, reduced biofilm formation by up to 70%. The treatment of C. jejuni cells with DAs changed the biofilm architecture and reduced the appearance of amyloid-like fibrils. In addition, a mixture of DAs enhanced antimicrobial efficacy of D-Cycloserine (DCS) up to 32% as compared with DCS treatment alone. Unexpectedly, D-alanine was able to reverse the inhibitory effect of other DAs as well as that of DCS. Furthermore, L-alanine and D-tryptophan decreased transcript levels of peptidoglycan biosynthesis enzymes alanine racemase (alr) and D-alanine-D-alanine ligase (ddlA) while D-serine was only able to decrease the transcript levels of alr. Our findings suggest that a combination of DAs could reduce biofilm formation, viability and persistence of C. jejuni through dysregulation of alr and ddlA.
Generation of Rat Monoclonal Antibody to Detect Hydrogen Sulfide and Polysulfides in Biological Samples
Authors: Kasamatsu Shingo , Kakihana Yuki , Koga Taisei , Yoshioka Hisashi , Ihara Hideshi
Published: November 2020

Hydrogen sulfide (H2S) is endogenously produced by enzymes and via reactive persulfide/polysulfide degradation; it participates in a variety of biological processes under physiological and pathological conditions. H2S levels in biological fluids, such as plasma and serum, are correlated with the severity of various diseases. Therefore, development of a simple and selective H2S measurement method would be advantageous. This study aimed to generate antibodies specifically recognizing H2S derivatives and develop a colorimetric immunoassay for measuring H2S in biological samples. We used N-ethylmaleimide (NEM) as an H2S detection agent that forms a stable bis-S-adduct (NEM-S-NEM). We also prepared bis-S-heteroadduct with 3-maleimidopropionic acid, which, in conjugation with bovine serum albumin, was to immunize Japanese white rabbits and Wistar rats to enable generation of polyclonal and monoclonal antibodies, respectively. The generated antibodies were evaluated by competitive enzyme-linked immunosorbent assay. We could obtain two stable hybridoma cell lines producing monoclonal antibodies specific for NEM-S-NEM. By immunoassay with the monoclonal antibody, the H2S level in mouse plasma was determined as 0.2 μM, which was identical to the level detected by mass spectrometry. Taken together, these monoclonal antibodies can be a useful tool for a simple and highly selective immunoassay to detect H2S in biological samples.
Oleacein Attenuates the Pathogenesis of Experimental Autoimmune Encephalomyelitis through Both Antioxidant and Anti-Inflammatory Effects
Authors: Gutiérrez-Miranda Beatriz , Gallardo Isabel , Melliou Eleni , Cabero Isabel , Álvarez Yolanda , Magiatis Prokopios , Hernández Marita , Nieto María Luisa
Published: November 2020

Oxidative stress and proinflammatory cytokines are factors affecting multiple sclerosis (MS) disease progression. Oleacein (OLE), an olive secoiridoid, possesses powerful antioxidant and anti-inflammatory activities, which suggests its potential application to treat neuroinflammatory disorders. Herein, we investigated the impact of OLE on the main clinic-pathological features of experimental autoimmune encephalomyelitis (EAE), an animal model for MS, including paralysis, demyelination, central nervous system (CNS) inflammation/oxidative stress and blood-brain barrier (BBB) breakdown. Methods: Mice were immunized with the myelin oligodendrocyte glycoprotein peptide, MOG35-55, to induce EAE, and OLE was administrated from immunization day. Serum, optic nerve, spinal cord and cerebellum were collected to evaluate immunomodulatory activities at a systemic level, as well as within the CNS. Additionally, BV2 microglia and the retinal ganglion cell line RGC-5 were used to confirm the direct effect of OLE on CNS-resident cells. Results: We show that OLE treatment effectively reduced clinical score and histological signs typical of EAE. Histological evaluation confirmed a decrease in leukocyte infiltration, demyelination, BBB disruption and superoxide anion accumulation in CNS tissues of OLE-treated EAE mice compared to untreated ones. OLE significantly decreased expression of proinflammatory cytokines (IL-13, TNFα, GM-CSF, MCP-1 and IL-1β), while it increased the anti-inflammatory cytokine IL-10. Serum levels of anti-MOG35-55 antibodies were also lower in OLE-treated EAE mice. Further, OLE significantly diminished the presence of oxidative system parameters, while upregulated the ROS disruptor, Sestrin-3. Mechanistically, OLE prevented NLRP3 expression, phosphorylation of p65-NF-κB and reduced the synthesis of proinflammatory mediators induced by relevant inflammatory stimuli in BV2 cells. OLE did not affect viability or the phagocytic capabilities of BV2 microglia. In addition, apoptosis of RGC-5 induced by oxidative stressors was also prevented by OLE. Conclusion: Altogether, our results show that the antioxidant and anti-inflammatory OLE has neuroprotective effects in the CNS of EAE mice, pointing out this natural product as a candidate to consider for research on MS treatments.
Profiling of Low-Molecular-Weight Carbonyls and Protein Modifications in Flavored Milk
Authors: Wölk Michele , Schröter Theres , Hoffmann Ralf , Milkovska-Stamenova Sanja
Published: November 2020

Thermal treatments of dairy products favor oxidations, Maillard reactions, and the formation of sugar or lipid oxidation products. Additives including flavorings might enhance these reactions or even induce further reactions. Here we aimed to characterize protein modifications in four flavored milk drinks using samples along the production chain—raw milk, pasteurization, mixing with flavorings, heat treatment, and the commercial product. Therefore, milk samples were analyzed using a bottom up proteomics approach and a combination of data-independent (MSE) and data-dependent acquisition methods (DDA). Twenty-one small carbonylated lipids were identified by shotgun lipidomics triggering 13 protein modifications. Additionally, two Amadori products, 12 advanced glycation end products (AGEs), and 12 oxidation-related modifications were targeted at the protein level. The most common modifications were lactosylation, formylation, and carboxymethylation. The numbers and distribution of modification sites present in raw milk remained stable after pasteurization and mixing with flavorings, while the final heat treatment significantly increased lactosylation and hexosylation in qualitative and quantitative terms. The processing steps did not significantly affect the numbers of AGE-modified, oxidized/carbonylated, and lipid-carbonylated sites in proteins.
A Posteriori Detection of Numerical Locking in hpq-Adaptive Finite Element Analysis
Authors: Miazio Łukasz , Zboiński Grzegorz
Published: November 2020

The proposed detection algorithms are assigned for the hpq-adaptive finite element analysis of the solid mechanics problems affected by the locking phenomena. The algorithms are combined with the M- and hpq-adaptive finite element method, where M is the element model, h denotes the element size parameter, and p and q stand for the longitudinal and transverse approximation orders within an element. The applied adaptive scheme is extended with the additional step where the locking phenomena are a posteriori detected, assessed and resolved. The detection can be applied to shear, membrane, or shear–membrane locking phenomena. The removal of the undesired influence of the numerical locking on the problem solution is based on p-enrichment of the mesh. The detection algorithm is also enriched with the locking assessment algorithm which is capable of determination of the optimized value of p which is sufficient for the phenomena removal. The detection and assessment algorithms are based on a simple sensitivity analysis performed locally for the finite elements of the thin-walled domain. The sensitivity analysis lies in comparison of the element solutions corresponding to two values of the order p, namely current and potentially eliminating the locking. The local solutions are obtained from the element residual method. The elaborated algorithms are original, relatively simple, extremely reliable, and highly effective.
A Voltage Multiplier Circuit Based Quadratic Boost Converter for Energy Storage Application
Authors: Ahmad Javed , Zaid Mohammad , Sarwar Adil , Lin Chang-Hua , Ahmad Shafiq , Sharaf Mohamed , Zaindin Mazen , Firdausi Muhammad
Published: November 2020

In this paper, a new transformerless high voltage gain dc-dc converter is proposed for low and medium power application. The proposed converter has high quadratic gain and utilizes only two inductors to achieve this gain. It has two switches that are operated simultaneously, making control of the converter easy. The proposed converter’s output voltage gain is higher than the conventional quadratic boost converter and other recently proposed high gain quadratic converters. A voltage multiplier circuit (VMC) is integrated with the proposed converter, which significantly increases the converter’s output voltage. Apart from a high output voltage, the proposed converter has low voltage stress across switches and capacitors, which is a major advantage of the proposed topology. A hardware prototype of 200 W of the proposed converter is developed in the laboratory to validate the converter’s performance. The efficiency of the converter is obtained through PLECS software by incorporating the switching and conduction losses.
Effects of a Combination of Elderberry and Reishi Extracts on the Duration and Severity of Respiratory Tract Infections in Elderly Subjects: A Randomized Controlled Trial
Authors: Gracián-Alcaide Carlos , Maldonado-Lobón Jose A. , Ortiz-Tikkakoski Elisabeth , Gómez-Vilchez Alejandro , Fonollá Juristo , López-Larramendi Jose L. , Olivares Mónica , Blanco-Rojo Ruth
Published: November 2020

Elderly people are particularly vulnerable to respiratory tract infections, so natural strategies to ameliorate the duration and severity of these infections are of great interest in this population. The objective of this study is to evaluate the efficacy of the consumption of a combination of elderberry and reishi extracts on the incidence, severity, and duration of respiratory tract infections in a group of healthy elderly volunteers. A randomized, double-blind, placebo-controlled pilot study was performed during the winter season. A group of 60 nursing home residents ≥65 years of age was randomly assigned to receive a combination of 1.5 g of elderberry +0.5 g of reishi or a placebo daily for 14 weeks. Data about the health conditions of the volunteers were evaluated and recorded by a medical doctor every 2 weeks. The incidence of respiratory infections was similar in both groups. However, volunteers in the extract group presented a significantly lower duration of common cold events (2.5 vs. 4.8 days, p = 0.033).and a significantly lower probability of having a high severity influenza-like illness event (p = 0.039). Moreover, the incidence of sleep disturbances was significantly lower in the extract group (p = 0.049). Therefore, the administration of a combination of elderberry and reishi extracts to the elderly population during the winter season might be used as a natural strategy to reduce the duration and severity of respiratory tract infections.
Impact of Osteotomy in Surgically Assisted Rapid Maxillary Expansion Using Tooth-Borne Appliance on the Formation of Stresses and Displacement Patterns in the Facial Skeleton—A Study Using Finite Element Analysis (FEA)
Authors: Zawiślak Ewa , Olejnik Anna , Frątczak Roman , Nowak Rafał
Published: November 2020

The analysis aimed at studying stresses reduced according to Huber’s hypothesis and displacement patterns at selected sites of the facial skeleton using a tooth-borne appliance in surgically assisted rapid maxillary expansion. Five different variants of osteotomy of the midface and a variant without surgical intervention were compared to determine the best model for making an incision in the maxilla. The finite element analysis (FEA) was used for the study. Five osteotomy variants and a variant without osteotomy were modelled using a tooth-borne appliance on a facial skeleton model of a 23-year-old woman with skeletal malocclusion. The finite element mesh was constructed based on the geometry imported into the ANSYS 15.0 (Swanson Analysis System of USA) software, in which calculations were performed using the finite element analysis. Stress distributions and displacement patterns along the X, Y and Z axes are presented for each osteotomy variant with the expansion of the tooth-borne appliance at a level of 0.5 mm. As a result of the analysis it was found that osteotomy of the palatal suture in conjunction with Le Fort I osteotomy has the biggest impact on the course of maxillary expansion. If no osteotomy is performed, an increase in stresses reduced according to Huber occurs in the entire facial skeleton with a simultaneous absence of maxillary expansion.
Effectiveness of Using a Patient Simulator with Real-Time Feedback to Improve Light-Curing Skills of Dental Students
Authors: Tauböck Tobias T. , Par Matej , Attin Thomas , Burrer Phoebe
Published: November 2020

The present study investigated the effectiveness of employing a patient simulator with an integrated visual feedback mechanism to improve the light-curing skills of dental students. A total of 44 third-year dental students were randomly divided into a control group (n = 22) and a training group (n = 22). Both groups light-cured a simulated restoration in tooth 27 in a patient simulator (MARC Patient Simulator, BlueLight Analytics, Halifax, Canada) by using the same curing device for 10 s. Delivered irradiances were recorded in real time by the built-in spectrophotometer. After measuring the baseline irradiances for both groups, the training group received detailed light-curing instructions and hands-on training with immediate visual feedback using the patient simulator. The irradiance of the training group was re-measured after the training. Both groups then attended a 26-day preclinical course, which involved placing 30 composite restorations. Upon completion of this course, the light-curing performance of both groups was re-assessed. The data were statistically analyzed using the Wilcoxon signed-rank test, Friedman’s ANOVA, and the Mann-Whitney U-test at an overall level of significance of α = 0.05. At baseline, the control and the training group delivered statistically similar irradiances with similar data scattering. In the training group, data scattering was considerably reduced after the hands-on training with the patient simulator. After the 26-day preclinical course, the irradiance of the training group was significantly higher and considerably less scattered compared to the control group. In conclusion, training with the patient simulator improved the light-curing performance of the dental students, mainly by helping them to deliver light energy more consistently.
Inductance Model of a Backside Integrated Power Inductor in 2.5D/3D Integration
Authors: Qian Kefang , Qian Libo
Published: November 2020

Inductor integration is of vital importance for miniaturization of power supply on chips. In this paper, a backside integrated power inductor is presented. The inductor is placed at the backside of a silicon interposer and connected to the front side metal layers by through-silicon vias (TSVs) for area saving and simple fabrication. An inductance model is proposed to effectively capture the total inductance of the power inductor by an analytical method. The results obtained from the analytical model and finite element method exhibit good agreement with various design parameters and the error between the proposed model and measurement remains less than 7.91%, which indicates that the proposed model can predict the inductance suitably.
Data-Driven Approach for Incident Management in a Smart City
Authors: Elvas Luís B. , Marreiros Carolina F. , Dinis João M. , Pereira Maria C. , Martins Ana L. , Ferreira João C.
Published: November 2020

Buildings in Lisbon are often the victim of several types of events (such as accidents, fires, collapses, etc.). This study aims to apply a data-driven approach towards knowledge extraction from past incident data, nowadays available in the context of a Smart City. We apply a Cross Industry Standard Process for Data Mining (CRISP-DM) approach to perform incident management of the city of Lisbon. From this data-driven process, a descriptive and predictive analysis of an events dataset provided by the Lisbon Municipality was possible, together with other data obtained from the public domain, such as the temperature and humidity on the day of the events. The dataset provided contains events from 2011 to 2018 for the municipality of Lisbon. This data mining approach over past data identified patterns that provide useful knowledge for city incident managers. Additionally, the forecasts can be used for better city planning, and data correlations of variables can provide information about the most important variables towards those incidents. This approach is fundamental in the context of smart cities, where sensors and data can be used to improve citizens’ quality of life. Smart Cities allow the collecting of data from different systems, and for the case of disruptive events, these data allow us to understand them and their cascading effects better.
Extended Use for the Frequency Response Analysis: Switching Impulse Voltage Based Preliminary Diagnosis of Potential Sources of Partial Discharges in Transformer
Authors: Arunachalam Kamalaselvan , Madanmohan Balasubramanian , Rajamani Rajesh , Prabaharan Natarajan , Haes Alhelou Hassan , Siano Pierluigi
Published: November 2020

The Frequency Response Analysis approach (FRA) is useful in the fault diagnosis of transformers. However, its usefulness in diagnosing any potential sources of Partial Discharge (PD) in transformers has not been thoroughly investigated so far. In this work, the use of Impulse voltage-based FRA (IFRA) in diagnosing inter-turn shorts and potential sources of PD were investigated on a 315 kVA, 11 kV/433 V transformer. Inter-turn shorts and PD sources were emulated and the usefulness of IFRA in their diagnosis was investigated while using switching impulse voltage at different magnitude levels as the test signals. For emulating the inter-turn shorts and the PDs, special tappings were provided on one of the 11 kV windings through the low capacitance bushings. Low voltage impulse was successful in diagnosing the inter-turn shorts, but unsuccessful in identifying the sources of PDs. During the test condition, the test voltage was adjusted with the presence of artificially created PD sources. The frequency response of the transformer before and after the inception of PD was observed and analyzed in this article. The FRA results demonstrated that the switching impulse voltage based IFRA approach at moderate voltages could be useful in diagnosing the presence of the potential sources of PDs.
Deep Learning-Based Pixel-Wise Lesion Segmentation on Oral Squamous Cell Carcinoma Images
Authors: Martino Francesco , Bloisi Domenico D. , Pennisi Andrea , Fawakherji Mulham , Ilardi Gennaro , Russo Daniela , Nardi Daniele , Staibano Stefania , Merolla Francesco
Published: November 2020

Oral squamous cell carcinoma is the most common oral cancer. In this paper, we present a performance analysis of four different deep learning-based pixel-wise methods for lesion segmentation on oral carcinoma images. Two diverse image datasets, one for training and another one for testing, are used to generate and evaluate the models used for segmenting the images, thus allowing to assess the generalization capability of the considered deep network architectures. An important contribution of this work is the creation of the Oral Cancer Annotated (ORCA) dataset, containing ground-truth data derived from the well-known Cancer Genome Atlas (TCGA) dataset.
Microgravity-Induced Cell-to-Cell Junctional Contacts Are Counteracted by Antioxidant Compounds in TCam-2 Seminoma Cells
Authors: Catizone Angela , Morabito Caterina , Cammarota Marcella , Schiraldi Chiara , Corano Scheri Katia , Ferranti Francesca , Mariggiò Maria , Ricci Giulia
Published: November 2020

The direct impact of microgravity exposure on male germ cells, as well as on their malignant counterparts, has not been largely studied. In previous works, we reported our findings on a cell line derived from a human seminoma lesion (TCam-2 cell line) showing that acute exposure to simulated microgravity altered microtubule orientation, induced autophagy, and modified cell metabolism stimulating ROS production. Moreover, we demonstrated that the antioxidant administration prevented both TCam-2 microgravity-induced microtubule disorientation and autophagy induction. Herein, expanding previous investigations, we report that simulated microgravity exposure for 24 h induced the appearance, at an ultrastructural level, of cell-to-cell junctional contacts that were not detectable in cells grown at 1 g. In line with this result, pan-cadherin immunofluorescence analyzed by confocal microscopy, revealed the clustering of this marker at the plasma membrane level on microgravity exposed TCam-2 cells. The upregulation of cadherin was confirmed by Western blot analyses. Furthermore, we demonstrated that the microgravity-induced ROS increase was responsible for the distribution of cadherin nearby the plasma membrane, together with beta-catenin since the administration of antioxidants prevented this microgravity-dependent phenomenon. These results shed new light on the microgravity-induced modifications of the cell adhesive behavior and highlight the role of ROS as microgravity activated signal molecules.
Deep-Learning-Based Computer-Aided Systems for Breast Cancer Imaging: A Critical Review
Authors: Jiménez-Gaona Yuliana , Rodríguez-Álvarez María José , Lakshminarayanan Vasudevan
Published: November 2020

This paper provides a critical review of the literature on deep learning applications in breast tumor diagnosis using ultrasound and mammography images. It also summarizes recent advances in computer-aided diagnosis/detection (CAD) systems, which make use of new deep learning methods to automatically recognize breast images and improve the accuracy of diagnoses made by radiologists. This review is based upon published literature in the past decade (January 2010–January 2020), where we obtained around 250 research articles, and after an eligibility process, 59 articles were presented in more detail. The main findings in the classification process revealed that new DL-CAD methods are useful and effective screening tools for breast cancer, thus reducing the need for manual feature extraction. The breast tumor research community can utilize this survey as a basis for their current and future studies.
High-Durability Concrete Using Eco-Friendly Slag-Pozzolanic Cements and Recycled Aggregate
Authors: Voit Klaus , Zeman Oliver , Janotka Ivan , Adamcova Renata , Bergmeister Konrad
Published: November 2020

Clinker production is very energy-intensive and responsible for releasing climate-relevant carbon dioxide (CO2) into the atmosphere, and the exploitation of aggregate for concrete results in a reduction in natural resources. This contrasts with infrastructure development, surging urbanization, and the demand for construction materials with increasing requirements in terms of durability and strength. A possible answer to this is eco-efficient, high-performance concrete. This article illustrates basic material investigations to both, using eco-friendly cement and recycled aggregate from tunneling to produce structural concrete and inner shell concrete, showing high impermeability and durability. By replacing energy- and CO2-intensive cement types by slag-pozzolanic cement (CEM V) and using recycled aggregate, a significant contribution to environmental sustainability can be provided while still meeting the material requirements to achieve a service lifetime for the tunnel structure of up to 200 years. Results of this research show that alternative cements (CEM V), as well as processed tunnel spoil, indicate good applicability in terms of their properties. Despite the substitution of conventional clinker and conventional aggregate, the concrete shows good workability and promising durability in conjunction with adequate concrete strengths.
Future Changes in the Free Tropospheric Freezing Level and Rain–Snow Limit: The Case of Central Chile
Authors: Mardones Piero , Garreaud René D.
Published: November 2020

The freezing level in the free troposphere often intercepts the terrain of the world’s major mountain ranges, creating a rain–snow limit. In this work, we use the free tropospheric height of the 0 °C isotherm (H0) as a proxy of both levels and study its distribution along the western slope of the subtropical Andes (30°–38° S) in present climate and during the rest of the 21st century. This portion of the Andes corresponds to central Chile, a highly populated region where warm winter storms have produced devastating landslides and widespread flooding in the recent past. Our analysis is based on the frequency distribution of H0 derived from radiosonde and surface observations, atmospheric reanalysis and climate simulations. The future projections primarily employ a scenario of heavy greenhouse gasses emissions (RCP8.5), but we also examine the more benign RCP4.5 scenario. The current H0 distribution along the central Chile coast shows a gradual decrease southward, with mean heights close to 2600 m ASL (above sea level) at 30 °C S to 2000 m ASL at 38° S for days with precipitation, about 800 m lower than during dry days. The mean value under wet conditions toward the end of the century (under RCP8.5) is close to, or higher than, the upper quartile of the H0 distribution in the current climate. More worrisome, H0 values that currently occur only 5% of the time will be exceeded in about a quarter of the rainy days by the end of the century. Under RCP8.5, even moderate daily precipitation can increase river flow to levels that are considered hazardous for central Chile.
Modeling Maximum Tsunami Heights Using Bayesian Neural Networks
Authors: Song Min-Jong , Cho Yong-Sik
Published: November 2020

Tsunamis are distinguished from ordinary waves and currents owing to their characteristic longer wavelengths. Although the occurrence frequency of tsunamis is low, it can contribute to the loss of a large number of human lives as well as property damage. To date, tsunami research has concentrated on developing numerical models to predict tsunami heights and run-up heights with improved accuracy because hydraulic experiments are associated with high costs for laboratory installation and maintenance. Recently, artificial intelligence has been developed and has revealed outstanding performance in science and engineering fields. In this study, we estimated the maximum tsunami heights for virtual tsunamis. Tsunami numerical simulation was performed to obtain tsunami height profiles for historical tsunamis and virtual tsunamis. Subsequently, Bayesian neural networks were employed to predict maximum tsunami heights for virtual tsunamis.
Engineering Human–Machine Teams for Trusted Collaboration
Authors: Alhaji Basel , Beecken Janine , Ehlers Rüdiger , Gertheiss Jan , Merz Felix , Müller Jörg P. , Prilla Michael , Rausch Andreas , Reinhardt Andreas , Reinhardt Delphine , Rembe Christian , Rohweder Niels-Ole , Schwindt Christoph , Westphal Stephan , Zimmermann Jürgen
Published: November 2020

The way humans and artificially intelligent machines interact is undergoing a dramatic change. This change becomes particularly apparent in domains where humans and machines collaboratively work on joint tasks or objects in teams, such as in industrial assembly or disassembly processes. While there is intensive research work on human–machine collaboration in different research disciplines, systematic and interdisciplinary approaches towards engineering systems that consist of or comprise human–machine teams are still rare. In this paper, we review and analyze the state of the art, and derive and discuss core requirements and concepts by means of an illustrating scenario. In terms of methods, we focus on how reciprocal trust between humans and intelligent machines is defined, built, measured, and maintained from a systems engineering and planning perspective in literature. Based on our analysis, we propose and outline three important areas of future research on engineering and operating human–machine teams for trusted collaboration. For each area, we describe exemplary research opportunities.
Transcriptional Responses of the Heat Shock Protein 20 (Hsp20) and 40 (Hsp40) Genes to Temperature Stress and Alteration of Life Cycle Stages in the Harmful Alga Scrippsiella trochoidea (Dinophyceae)
Authors: Deng Yunyan , Hu Zhangxi , Shang Lixia , Chai Zhaoyang , Tang Ying Zhong
Published: November 2020

The small heat shock protein (sHsp) and Hsp40 are Hsp members that have not been intensively investigated but are functionally important in most organisms. In this study, the potential roles of a Hsp20 (StHsp20) and a Hsp40 (StHsp40) in dinoflagellates during adaptation to temperature fluctuation and alteration of different life stages were explored using the representative harmful algal blooms (HABs)-causative dinoflagellate species, Scrippsiella trochoidea. We isolated the full-length cDNAs of the two genes via rapid amplification of cDNA ends (RACE) and tracked their differential transcriptions via real-time qPCR. The results revealed StHsp20 and StHsp40 exhibited mRNA accumulation patterns that were highly similar in response to heat stress but completely different toward cold stress, which implies that the mechanisms underlying thermal and cold acclimation in dinoflagellates are regulated by different sets of genes. The StHsp20 was probably related to the heat tolerance of the species, and StHsp40 was closely involved in the adaptation to both higher and lower temperature fluctuations. Furthermore, significantly higher mRNA abundance of StHsp40 was detected in newly formed resting cysts, which might be a response to intrinsic stress stemmed from encystment. This finding also implied StHsp40 might be engaged in resting cyst formation of S. trochoidea. Our findings enriched the knowledge about possible cross-talk of different Hsp members in dinoflagellates and provided clues to further explore the molecular underpinnings underlying resting cyst production and broad temperature tolerance of this group of HABs contributors.
Interaction between Laser Light and Osteoblasts: Photobiomodulation as a Trend in the Management of Socket Bone Preservation—A Review
Authors: Amaroli Andrea , Colombo Esteban , Zekiy Angelina , Aicardi Stefano , Benedicenti Stefano , De Angelis Nicola
Published: November 2020

Bone defects are the main reason for aesthetic and functional disability, which negatively affect patient’s quality of life. Particularly, after tooth extraction, the bone of the alveolar process resorbs, limiting the optimal prosthetic implant placement. One of the major pathophysiological events in slowly- or non-healing tissues is a blood supply deficiency, followed by a significant decrease in cellular energy amount. The literature shows that photons at the red and infrared wavelengths can interact with specific photoacceptors located within the cell. Through this mechanism, photobiomodulation (PBM) can modify cellular metabolism, by increasing mitochondrial ATP production. Here, we present a review of the literature on the effect of PBM on bone healing, for the management of socket preservation. A search strategy was developed in line with the PRISMA statement. The PubMed and Scholar electronic databases were consulted to search for in vivo studies, with restrictions on the year (<50 years-old), language (English), bone socket preservation, and PBM. Following the search strategy, we identified 269 records, which became 14, after duplicates were removed and titles, abstract and inclusion-, exclusion-criteria were screened. Additional articles identified were 3. Therefore, 17 articles were included in the synthesis. We highlight the osteoblast–light interaction, and the in vivo therapeutic tool of PBM is discussed.
Sustainable Antibiotic-Free Broiler Meat Production: Current Trends, Challenges, and Possibilities in a Developing Country Perspective
Authors: Haque Md. Hakimul , Sarker Subir , Islam Md. Shariful , Islam Md. Aminul , Karim Md. Rezaul , Kayesh Mohammad Enamul Hoque , Shiddiky Muhammad J. A. , Anwer M. Sawkat
Published: November 2020

Antibiotic-free broiler meat production is becoming increasingly popular worldwide due to consumer perception that it is superior to conventional broiler meat. Globally, broiler farming impacts the income generation of low-income households, helping to alleviate poverty and secure food in the countryside and in semi-municipal societies. For decades, antibiotics have been utilized in the poultry industry to prevent and treat diseases and promote growth. This practice contributes to the development of drug-resistant bacteria in livestock, including poultry, and humans through the food chain, posing a global public health threat. Additionally, consumer demand for antibiotic-free broiler meat is increasing. However, there are many challenges that need to be overcome by adopting suitable strategies to produce antibiotic-free broiler meat with regards to food safety and chicken welfare issues. Herein, we focus on the importance and current scenario of antibiotic use, prospects, and challenges in the production of sustainable antibiotic-free broiler meat, emphasizing broiler farming in the context of Bangladesh. Moreover, we also discuss the need for and challenges of antibiotic alternatives and provide a future outlook for antibiotic-free broiler meat production.
Molybdenum Supply Alleviates the Cadmium Toxicity in Fragrant Rice by Modulating Oxidative Stress and Antioxidant Gene Expression
Authors: Imran Muhammad , Hussain Saddam , El-Esawi Mohamed A. , Rana Muhammad Shoaib , Saleem Muhammad Hamzah , Riaz Muhammad , Ashraf Umair , Potcho Mouloumdema Pouwedeou , Duan Meiyang , Rajput Imran Ali , Tang Xiangru
Published: November 2020

Increasing evidence shows that cadmium (Cd) toxicity causes severe perturbations on growth performance, physio-biochemical and molecular processes in crop plants. Molybdenum (Mo), an essential trace element, plays key roles in oxidative stress tolerance of higher plants. Hence, the present study has been conducted to investigate the possible role of Mo in alleviating Cd-induced inhibitions in two fragrant rice cultivars namely Guixiangzhan (GXZ) and Meixiangzhan-2 (MXZ-2). The results revealed that Mo application enhanced the plant dry biomass by 73.24% in GXZ and 58.09% in MXZ-2 under Cd stress conditions, suggesting that Mo supplementation alleviated Cd-induced toxicity effects in fragrant rice. The enhanced Cd-tolerance in fragrant rice plants prompted by Mo application could be ascribed to its ability to regulate Cd uptake and reduce Cd-induced oxidative stress as evident by lower hydrogen peroxide levels, electrolyte leakage and malondialdehyde contents in Cd-stressed plants. The ameliorative role of Mo against Cd-toxicity also reflected through its protection to the photosynthetic pigments, proline and soluble protein. Mo also induced antioxidant defense systems via maintaining higher contents of glutathione and ascorbate as well as enhancing the ROS-detoxifying enzymes such as catalase, peroxidase, superoxide dismutase and ascorbate peroxidase activities and up-regulating transcript abundance in both fragrant rice cultivars under Cd stress. Conclusively, Mo-mediated modulation of Cd toxicity in fragrant rice was through restricting Cd uptake, maintaining photosynthetic performance and alleviating oxidative damages via the strong anti-oxidative defense systems; however, GXZ cultivar is comparatively more Cd tolerant and Mo-efficient as evident from the less growth inhibition and biomass reduction as well as enhanced Mo-induced Cd stress tolerance and less oxidative damage than MXZ-2 fragrant rice cultivar.