bluuebunny/crossref_metadata_2025 huggingface dataset:
Investigation of the Affinity of Ceftobiprole for Selected Cyclodextrins Using Molecular Dynamics Simulations and HPLC
Authors: Boczar Dariusz , Michalska Katarzyna
Published: November 2023

This paper presents the theoretical calculations of the inclusion complex formation between native ceftobiprole, a promising antibiotic from the cephalosporin group, and selected cyclodextrins (CDs) approved by the European Medicines Agency. Ceftobiprole was studied in three protonation states predicted from pKa calculations, along with three selected CDs in a stoichiometric ratio of 1:1. It was introduced into the CD cavity in two opposite directions, resulting in 18 possible combinations. Docking studies determined the initial structures of the complexes, which then served as starting structures for molecular dynamics simulations. The analysis of the obtained trajectories included the spatial arrangement of ceftobiprole and CD, the hydrogen bonds forming between them, and the Gibbs free energy (ΔG) of the complex formation, which was calculated using the Generalised Born Surface Area (GBSA) equation. Among them, a complex of sulfobutyl ether- (SBE-) β-CD with protonated ceftobiprole turned out to be the most stable (ΔG = −12.62 kcal/mol = −52.80 kJ/mol). Then, experimental studies showed changes in the physiochemical properties of the ceftobiprole in the presence of the CDs, thus confirming the validity of the theoretical results. High-performance liquid chromatography analysis showed that the addition of 10 mM SBE-β-CD to a 1 mg/mL solution of ceftobiprole in 0.1 M of HCl increased the solubility 1.5-fold and decreased the degradation rate constant 2.5-fold.
Accumulation of 4-Hydroxynonenal Characterizes Diabetic Fat and Modulates Adipogenic Differentiation of Adipose Precursor Cells
Authors: Murdolo Giuseppe , Bartolini Desirée , Tortoioli Cristina , Vermigli Cristiana , Piroddi Marta , Galli Francesco
Published: November 2023

Redox imbalance in fat tissue appears to be causative of impaired glucose homeostasis. This “proof-of-concept” study investigated whether the peroxidation by-product of polyunsaturated n-6 fatty acids, namely 4-hydroxynonenal (4-HNE), is formed by, and accumulates in, the adipose tissue (AT) of obese patients with type 2 diabetes (OBT2D) as compared with lean, nondiabetic control subjects (CTRL). Moreover, we studied the effects of 4-HNE on the cell viability and adipogenic differentiation of adipose-derived stem cells (ASCs). Protein–HNE adducts in subcutaneous abdominal AT (SCAAT) biopsies from seven OBT2D and seven CTRL subjects were assessed using Western blot. The effects of 4-HNE were then studied in primary cultures of ASCs, focusing on cell viability, adipogenic differentiation, and the “canonical” Wnt and MAPK signaling pathways. When compared with the controls, the OBT2D patients displayed increased HNE–protein adducts in the SCAAT. The exposure of ASCs to 4-HNE fostered ROS production and led to a time- and concentration-dependent decrease in cell viability. Notably, at concentrations that did not affect cell viability (1 μM), 4-HNE hampered adipogenic ASCs’ differentiation through a timely-regulated activation of the Wnt/β-catenin, p38MAPK, ERK1/2- and JNK-mediated pathways. These “hypothesis-generating” data suggest that the increased accumulation of 4-HNE in the SCAAT of obese patients with type 2 diabetes may detrimentally affect adipose precursor cell differentiation, possibly contributing to the obesity-associated derangement of glucose homeostasis.
CRISPR/Cas as a Genome-Editing Technique in Fruit Tree Breeding
Authors: Martín-Valmaseda Marina , Devin Sama Rahimi , Ortuño-Hernández Germán , Pérez-Caselles Cristian , Mahdavi Sayyed Mohammad Ehsan , Bujdoso Geza , Salazar Juan Alfonso , Martínez-Gómez Pedro , Alburquerque Nuria
Published: November 2023

CRISPR (short for “Clustered Regularly Interspaced Short Palindromic Repeats”) is a technology that research scientists use to selectively modify the DNA of living organisms. CRISPR was adapted for use in the laboratory from the naturally occurring genome-editing systems found in bacteria. In this work, we reviewed the methods used to introduce CRISPR/Cas-mediated genome editing into fruit species, as well as the impacts of the application of this technology to activate and knock out target genes in different fruit tree species, including on tree development, yield, fruit quality, and tolerance to biotic and abiotic stresses. The application of this gene-editing technology could allow the development of new generations of fruit crops with improved traits by targeting different genetic segments or even could facilitate the introduction of traits into elite cultivars without changing other traits. However, currently, the scarcity of efficient regeneration and transformation protocols in some species, the fact that many of those procedures are genotype-dependent, and the convenience of segregating the transgenic parts of the CRISPR system represent the main handicaps limiting the potential of genetic editing techniques for fruit trees. Finally, the latest news on the legislation and regulations about the use of plants modified using CRISPR/Cas systems has been also discussed.
Effects of Coverlys TF150® on the Photosynthetic Characteristics of Grape
Authors: Li Zhonghan , Jiang Enshun , Liu Minghui , Sun Qinghua , Gao Zhen , Du Yuanpeng
Published: November 2023

Grape rain-shelter cultivation is a widely employed practice in China. At present, the most commonly used rain shelter film materials are polyvinyl chloride (PVC), polyethylene (PE), ethylene-vinyl acetate copolymer (EVA), and polyolefin (PO). Coverlys TF150® is a woven fabric with an internal antifoggy PE coating that has not yet been popularized as a rain shelter film for grapes in China. To investigate the effects of Coverlys TF150® on grapes, we measured the microdomain environment, leaf development, and photosynthetic characteristics of ‘Miguang’ (Vitis vinifera × V. labrusca) under rain-shelter cultivation and performed transcriptome analysis. The results showed that Coverlys TF150® significantly reduced (p < 0.05) the light intensity, temperature, and humidity compared with PO film, increased the chlorophyll content and leaf thickness (particularly palisade tissue thickness), and increased stomatal density and stomatal opening from 10:00 to 14:00. Coverlys TF150® was observed to improve the maximum efficiency of photosystem II (Fv/Fm), photochemical quenching (qP), the electron transfer rate (ETR), and the actual photochemical efficiency (ΦPSII) from 10:00 to 14:00. Moreover, the net photosynthetic rate (Pn), intercellular CO2 concentration (Ci), stomatal conductance (Gs), and transpiration rate (Tr) of grape leaves significantly increased (p < 0.05) from 10:00 to 14:00. RNA-Seq analysis of the grape leaves at 8:00, 10:00, and 12:00 revealed 1388, 1562, and 1436 differential genes at these points in time, respectively. KEGG enrichment analysis showed the occurrence of protein processing in the endoplasmic reticulum. Plant hormone signal transduction and plant-pathogen interaction were identified as the metabolic pathways with the highest differential gene expression enrichment. The psbA encoding D1 protein was significantly up-regulated in both CO10vsPO10 and CO12vsPO12, while the sHSPs family genes were significantly down-regulated in all time periods, and thus may play an important role in the maintenance of the photosystem II (PSII) activity in grape leaves under Coverlys TF150®. Compared with PO film, the PSI-related gene psaB was up-regulated, indicating the ability of Coverlys TF150® to better maintain PSI activity. Compared with PO film, the abolic acid receptacle-associated gene PYL1 was down-regulated at all time periods under the Coverlys TF150® treatment, while PP2C47 was significantly up-regulated in CO10vsPO10 and CO12vsPO12, inducing stomatal closure. The results reveal that Coverlys TF150® alleviates the stress of high temperature and strong light compared with PO film, improves the photosynthetic capacity of grape leaves, and reduces the midday depression of photosynthesis.
Microbiota in Autism Spectrum Disorder: A Systematic Review
Authors: Lewandowska-Pietruszka Zuzanna , Figlerowicz Magdalena , Mazur-Melewska Katarzyna
Published: November 2023

Autism spectrum disorder (ASD) is a complex neurodevelopmental condition characterized by several core symptoms: restricted interests, communication difficulties, and impaired social interactions. Many ASD children experience gastrointestinal functional disorders, impacting their well-being. Emerging evidence suggests that a gut microbiota imbalance may exacerbate core and gastrointestinal symptoms. Our review assesses the gut microbiota in children with ASD and interventions targeting microbiota modulation. The analysis of forty-four studies (meta-analyses, reviews, original research) reveals insights into the gut microbiota–ASD relationship. While specific microbiota alterations are mixed, some trends emerge. ASD children exhibit increased Firmicutes (36–81%) and Pseudomonadota (78%) and decreased Bacteroidetes (56%). The Bacteroidetes to Firmicutes ratio tends to be lower (56%) compared to children without ASD, which correlates with behavioral and gastrointestinal abnormalities. Probiotics, particularly Lactobacillus, Bifidobacterium, and Streptococcus strains, show promise in alleviating behavioral and gastrointestinal symptoms (66%). Microbiota transfer therapy (MTT) seems to have lasting benefits for the microbiota and symptoms in one longitudinal study. Prebiotics can potentially help with gastrointestinal and behavioral issues, needing further research for conclusive efficacy due to different interventions being used. This review highlights the gut microbiota–ASD interplay, offering potential therapeutic avenues for the gut–brain axis. However, study heterogeneity, small sample sizes, and methodological variations emphasize the need for comprehensive, standardized research. Future investigations may unveil complex mechanisms linking the gut microbiota to ASD, ultimately enhancing the quality of life for affected individuals.
SOX18 Promotes the Proliferation of Dermal Papilla Cells via the Wnt/β-Catenin Signaling Pathway
Authors: He Mingliang , Lv Xiaoyang , Cao Xiukai , Yuan Zehu , Getachew Tesfaye , Li Yutao , Wang Shanhe , Sun Wei
Published: November 2023

SRY-box transcription factor 18 (SOX18) is known to play a crucial role in the growth and development of hair follicles (HF) in both humans and mice. However, the specific effect of SOX18 on sheep hair follicles remains largely unknown. In our previous study, we observed that SOX18 was specifically expressed within dermal papilla cells (DPCs) in ovine hair follicles, leading us to investigate its potential role in the growth of hair follicles in sheep. In the present study, we aimed to examine the effect of SOX18 in DPCs and preliminarily study its regulatory mechanism through RNA-seq. We initially found that the overexpression of SOX18 promoted the proliferation of DPCs compared to the negative control group, while the interference of SOX18 had the opposite effect. To gain further insight into the regulatory mechanism of SOX18, we conducted RNA-seq analysis after knocking down SOX18 in Hu sheep DPCs. The result showed that the Wnt/β-Catenin signaling pathway was involved in the growth process of DPC after SOX18 knockdown. Subsequently, we investigated the effect of SOX18 on the Wnt/β-Catenin signaling pathway in DPCs using TOP/FOP-flash, qRT-PCR, and Western blot (WB) analysis. Our data demonstrated that SOX18 could activate the Wnt/β-Catenin signaling pathway in DPCs. Additionally, we observed that SOX18 could rescue the proliferation of DPCs after inhibiting the Wnt/β-Catenin signaling pathway. These findings underscore the essential role of SOX18 as a functional molecule governing the proliferation of DPCs. Additionally, these findings also greatly enhance our understanding of the role of SOX18 in the proliferation of DPCs and the growth of wool in Hu sheep.
Genomic and Metabolic Characterization of Plant Growth-Promoting Rhizobacteria Isolated from Nodules of Clovers Grown in Non-Farmed Soil
Authors: Wójcik Magdalena , Koper Piotr , Żebracki Kamil , Marczak Małgorzata , Mazur Andrzej
Published: November 2023

The rhizosphere microbiota, which includes plant growth-promoting rhizobacteria (PGPR), is essential for nutrient acquisition, protection against pathogens, and abiotic stress tolerance in plants. However, agricultural practices affect the composition and functions of microbiota, reducing their beneficial effects on plant growth and health. Among PGPR, rhizobia form mutually beneficial symbiosis with legumes. In this study, we characterized 16 clover nodule isolates from non-farmed soil to explore their plant growth-promoting (PGP) potential, hypothesizing that these bacteria may possess unique, unaltered PGP traits, compared to those affected by common agricultural practices. Biolog profiling revealed their versatile metabolic capabilities, enabling them to utilize a wide range of carbon and energy sources. All isolates were effective phosphate solubilizers, and individual strains exhibited 1-aminocyclopropane-1-carboxylate deaminase and metal ion chelation activities. Metabolically active strains showed improved performance in symbiotic interactions with plants. Comparative genomics revealed that the genomes of five nodule isolates contained a significantly enriched fraction of unique genes associated with quorum sensing and aromatic compound degradation. As the potential of PGPR in agriculture grows, we emphasize the importance of the molecular and metabolic characterization of PGP traits as a fundamental step towards their subsequent application in the field as an alternative to chemical fertilizers and supplements.
Hyponatremia Promotes Cancer Growth in a Murine Xenograft Model of Neuroblastoma
Authors: Marroncini Giada , Naldi Laura , Fibbi Benedetta , Errico Alice , Polvani Simone , Brogi Marco , Fanelli Alessandra , Maggi Mario , Peri Alessandro
Published: November 2023

In cancer patients, hyponatremia is detected in about 40% of cases at hospital admission and has been associated to a worse outcome. We have previously observed that cancer cells from different tissues show a significantly increased proliferation rate and invasion potential, when cultured in low extracellular [Na+]. We have recently developed an animal model of hyponatremia using Foxn1nu/nu mice. The aim of the present study was to compare tumor growth and invasivity of the neuroblastoma cell line SK-N-AS in hyponatremic vs. normonatremic mice. Animals were subcutaneously implanted with luciferase-expressing SK-N-AS cells. When masses reached about 100 mm3, hyponatremia was induced in a subgroup of animals via desmopressin infusion. Tumor masses were significantly greater in hyponatremic mice, starting from day 14 and until the day of sacrifice (day 28). Immunohistochemical analysis showed a more intense vascularization and higher levels of expression of the proliferating cell nuclear antigen, chromogranin A and heme oxigenase-1 gene in hyponatremic mice. Finally, metalloproteases were also more abundantly expressed in hyponatremic animals compared to control ones. To our knowledge, this is the first demonstration in an experimental animal model that hyponatremia is associated to increased cancer growth by activating molecular mechanisms that promote proliferation, angiogenesis and invasivity.
Research on Sleep Dynamics in Cleft Lip and Palate Patients Using Simple Sleep Testing
Authors: Nemoto Naoko , Kawanabe Hitoshi , Fukui Kazunori , Oyama Akihiko , Okamoto Toru , Shimamura Kazuhiro
Published: November 2023

Sleep-disordered breathing affects children’s growth and development, mental health, and learning ability. Postoperative scarring causes anteroposterior and vertical developmental disorders of the maxilla. Obstructive apnea is likely to occur due to the influence on the maxillofacial and airway morphology. In this study, we investigated the sleep-respiratory dynamics of school-aged children with unilateral cleft lip and palate by performing a simple overnight sleep study, maxillofacial morphology, airway analysis using lateral cranial radiographs, and model analysis. Children with unilateral cleft lip and palate showed a significantly higher respiratory event index (REI) than normal children; the maxilla was located in the posterior position in terms of maxillofacial morphology and airway morphology showed narrow values for all distance measurement items. Moreover, the width and length of the dental arch and the width of the alveolar base arch were significantly smaller. Furthermore, REI and SNA, ANB, and REI were negatively correlated with alveolar base arch width. Children with unilateral cleft lip and palate are more likely than normal children to develop sleep-disordered breathing due to increased airway resistance caused by undergrowth of the maxilla and narrowing of the upper airway and oral volume.
Tailored Time–Temperature Transformation Diagram for IN718 Alloy Obtained via Powder Bed Fusion Additive Manufacturing: Phase Behavior and Precipitation Dynamic
Authors: Franco-Correa Julio Cesar , Martínez-Franco Enrique , Cruz-González Celso Eduardo , Salgado-López Juan Manuel , Villada-Villalobos Jhon Alexander
Published: November 2023

Experimental and computational approaches were used to study the microstructure of IN718 produced via powder bed fusion additive manufacturing (PBF-AM). The presence, chemical composition, and distribution of stable and metastable phases (γ′′, δ, MC, and Laves) were also analyzed. The information obtained from the microstructural study was used to construct a tailored time–temperature transformation (TTT) diagram customized for additive manufacturing of IN718. Experimental techniques, including differential scanning calorimetry (DSC), scanning electron microscopy, energy dispersive X-ray spectroscopy, and electron backscatter diffraction (EBSD), were employed to establish the morphological, chemical, and structural characteristics of the microstructure. The Thermo-Calc software and a Scheil–Gulliver model were used to analyze the presence and behavior of phase transformations during heating and cooling processes under non-thermodynamic equilibrium conditions, typical of AM processes. Unlike conventional TTT diagrams of this alloy, the diagram presented here reveals that the precipitation of γ′′ and δ phases occurs at lower temperatures and shorter times in AM-manufactured parts. Significantly, the superposition of γ′′ and δ phase curves in the proposed diagram underscores the interdependence between these phases. This TTT diagram is a valuable insight that can help in the development of heat treatment processes and quality control for IN718 produced via PBF-AM.
Study on the Effect of Recycled Fine Aggregate Qualities on Fly Ash/GGBS-Based Geopolymer Mortar
Authors: Liu Shilun , Liu Zihao , Takasu Koji , Koyamada Hidehiro , Suyama Hiroki
Published: November 2023

The rapid expansion of construction, fueled by industry and economic and population growth, has exacerbated the challenge of managing construction waste, especially concrete waste. One promising solution lies in the utilization of recycled fine aggregate (RFA), especially in combination with the emerging geopolymer technology, an innovative alternative to traditional cement. This study systematically explores the effects of incorporating varying qualities and quantities of RFA into geopolymer mortars. By using GGBS and FA as raw materials and replacing natural aggregates (NA) with RFA at different rates (25%, 50%, 75%, and 100%), the research investigates the fresh properties, mechanical characteristics, and drying shrinkage of geopolymer mortar. Key findings reveal that RFA significantly influences the flowability of geopolymer mortar: when RFA content is above 75%, preprocessed RFA (with particles below 0.15 mm removed) has substantially improved flowability, increasing it more than 20%. The critical impact of RFA preprocessing on enhancing mechanical properties and the higher the inclusion level (above 75%), the more pronounced is the advantage in enhancing the compressive strength compared to unprocessed RFA. Additionally, RFA was found to contribute to a denser interfacial transition zone (ITZ) than natural aggregate, which helps maintain the compressive strength at increased RFA dosages. Contrary to findings in cement mortar, a positive correlation exists between pore volume and compressive strength in geopolymer mortar incorporating RFA. This study underscores the potential of refined RFA preprocessing methods in advancing sustainable construction, highlighting avenues for the broader application of RFA in geopolymer mortar.
Investigation of TaC and TiC for Particle Strengthening of Co-Re-Based Alloys
Authors: Seif Eugen , Rösler Joachim , Werner Jonas , Weirich Thomas E. , Mayer Joachim
Published: November 2023

Cobalt-Rhenium (Co-Re)-based alloys are currently investigated as potential high-temperature materials with melting temperatures beyond those of nickel-based superalloys. Their attraction stems from the binary Co-Re phase diagram, exhibiting complete miscibility between Co and Re, whereby the melting temperature steadily increases with the Re-content. Thus, depending on the Re-content, one can tune the melting temperature between that of pure Co (1495 °C) and that of pure Re (3186 °C). Current investigations focus on Re-contents of about 15 at.%, which makes melting with standard equipment still feasible. In addition to solid solution strengthening due to the mixture of Co- and Re-atoms, particle strengthening by tantalum carbide (TaC) and titanium carbide (TiC) precipitates turned out to be promising in recent studies. Yet, it is currently unclear which of the two particle types is the best choice for high temperature applications nor has the strengthening mechanism associated with the monocarbide (MC)-precipitates been elucidated. To address these issues, we perform compression tests at ambient and elevated temperatures on the particle-free base material containing 15 at.% of rhenium (Re), 5 at.% of chromium (Cr) and cobalt (Co) as balance (Co-15Re-5Cr), as well as on TaC- and TiC-containing variants. Additionally, transmission electron microscopy is used to analyze the shape of the precipitates and their orientation relationship to the matrix. Based on these investigations, we show that TiC and TaC are equally suited for precipitation strengthening of Co-Re-based alloys and identify climb over the elongated particles as a rate controlling particle strengthening mechanism at elevated temperatures. Furthermore, we show that the Re-atoms are remarkably strong obstacles to dislocation motion, which are overcome by thermal activation at elevated temperatures.
Hardware–Software Embedded System for Real-Time Trajectory Planning of Multi-Axis Machine Using B-Spline Curve Interpolation Algorithm
Authors: Tan Qitao , Mohd Basri Mohd Ariffanan
Published: November 2023

This paper proposes a B-spline trajectory algorithm to realize multi-axis trajectory interpolation and analyzes the operating accuracy in an embedded system. However, the existing trajectory generation method needs to use computer-aided manufacturing (CAM) software to convert the interpolating trajectory into G code and download the code into the computer numerical control (CNC) system for processing. In this paper, the method of third-degree B-spline interpolation is proposed to generate a curved surface trajectory, and the trajectory generated by this algorithm can be run directly into a CNC system. The precision analysis of the ISO parameter segmentation interpolation algorithm and the theory of constant velocity motion is also presented. The significance of this project is that it designs a complete set of embedded systems, including hardware circuit design and software logic design, and uses low-cost STM32 architecture to realize a B-spline constant-speed interpolation algorithm, which is verified on CNC polishing equipment. A simulation conducted with the MATLAB software and the B-spline curve interpolation experiments performed on a multi-axis polishing machine tool demonstrate the effectiveness and accuracy of the optimized third-degree B-spline algorithm.
Induction of Browning in White Adipocytes: Fucoidan Characterization and Gold Nanoparticle Synthesis from Undaria pinnatifida Sporophyll Extract
Authors: Park Sun Young , Park Kangmin , Kang Hye Mi , Song Woo Chang , Oh Jin-Woo , Choi Young-Whan , Park Geuntae
Published: November 2023

Seaweed extracts and their specific polysaccharides are widely known for their ability to act as reducing and capping agents during nanoparticle synthesis. Their application is highly favored in green synthesis methods, owing to their eco-friendliness, cost-effectiveness, and remarkable time and energy efficiency. In this study, fucoidan extracted from Undaria pinnatifida sporophyll (UPS) is introduced as a polysaccharide that effectively serves as a dual-function reducing and capping agent for the synthesis of gold nanoparticles (AuNPs). Results from various analyses indicate that AuNPs derived from UPS extract display a uniform spherical shape with an average size of 28.34 ± 1.15 nm and a zeta potential of −37.49 ± 2.13 mV, conclusively confirming the presence of Au. The FT-IR spectra distinctly revealed the characteristic fucoidan bands on the stabilized UPS-AuNPs surface. A 1H-NMR analysis provided additional confirmation by revealing the presence of specific fucoidan protons on the UPS-AuNPs surface. To comprehensively evaluate the impact of UPS extract, UPS-AuNPs, and fucoidan on the biological properties of adipocytes, a rigorous comparative analysis of lipid droplet formation and morphology was conducted. Our findings revealed that adipocytes treated with UPS extract, fucoidan, and UPS-AuNPs, in that order, exhibited a reduction in the total lipid droplet surface area, maximum Ferret diameter, and overall Nile red staining intensity when compared to mature white adipocytes. Furthermore, our analysis of the effects of UPS extracts, UPS-AuNPs, and fucoidan on the expression of key markers associated with white adipose tissue browning, such as UCP1, PGC1a, and PRDM16, demonstrated increased mRNA and protein expression levels in the following order: UPS-AuNPs > fucoidan > UPS extracts. Notably, the production of active mitochondria, which play a crucial role in enhancing energy expenditure in beige adipocytes, also increased in the following order: UPS-AuNPs > fucoidan > UPS extract. These findings underscore the pivotal role of UPS extract, fucoidan, and UPS-AuNPs in promoting adipocyte browning and subsequently enhancing energy expenditure.
Unveiling the Therapeutic Potential and Healthcare Applications of Marine Therapy: A Systematic Review with Meta-Analysis and Meta-Regression
Authors: Shim Sung Ryul , Shin Dayeon , Kim Seong-Jang , Kim Young Kook , Lee Kyung Ju
Published: November 2023

This study was conducted to estimate the effectiveness of marine-derived resources for treating specific diseases, as well as identify the most effective methods for applying such resources in therapeutic applications. Bibliographic databases (PubMed, Embase, and Cochrane) were searched from their inception until May 2023 using Medical Subject Headings terms and text keywords related to seawater, mineral water, or ocean therapy. Fifteen eligible studies were included, involving 1325 participants aged 42.7–63.0 years. In the subgroup analysis based on treatment type, the mean difference was −1.581 (95% CI: −1.889, −1.274) for seawater with sun exposure and −1.210 (95% CI: −1.417, −1.002) for seawater with sun exposure, mud pack application, and sulfur pool therapy. The pooled standardized mean difference was calculated for different outcomes; the results were −1.110 (95% CI: −3.028, 0.806) for osteoarthritis severity, −0.795 (95% CI: −0.982, −0.607) for arthritis pain, −1.623 (95% CI: −2.036, −1.209) for fibromyalgia pain, and −1.498 (95% CI: −1.888, −1.108) for quality of life. Marine therapy is, therefore, promising for treating chronic skin issues, easing musculoskeletal discomfort, and enhancing the quality of life among patients with musculoskeletal pain.
Comparative Analysis of the Metabolic Profiles of Strains of Tribolium castaneum (Herbst) Adults with Different Levels of Phosphine Resistance Based on Direct Immersion Solid-Phase Microextraction and Gas Chromatography-Mass Spectrometry
Authors: Li Li , Shan Changyao , Liu Qun , Li Baishu , Liu Tao
Published: November 2023

The management of phosphine (PH3) resistance in stored grain pests is an essential component of implementing timely and effective pest control strategies. The prevailing standard method for PH3 resistance testing involves the exposure of adult insects to a specific concentration over a fixed period. Although it is widely adopted, this method necessitates an extensive period for assay preparation and diagnosis. To address this issue, this study employed Direct Immersion Solid-Phase Microextraction (DI-SPME) coupled with Gas Chromatography-Mass Spectrometry (GC-MS) to compare and analyze the metabolic profiles of PH3-sensitive (TC-S), PH3 weak-resistant (TC-W), and PH3 strong-resistant (TC-SR) Tribolium castaneum (Herbst) adults. A total of 36 metabolites were identified from 3 different PH3-resistant strains of T. castaneum; 29 metabolites were found to present significant differences (p < 0.05) across these groups, with hydrocarbon and aromatic compounds being particularly prevalent. Seven metabolites showed no significant variations among the strains, consisting of four hydrocarbon compounds, two iodo-hydrocarbon compounds, and one alcohol compound. Further multivariate statistical analysis revealed a total of three, two, and nine differentially regulated metabolites between the TC-S versus TC-W, TC-S versus TC-SR, and TC-W versus TC-SR groups, respectively. Primarily, these metabolites comprised hydrocarbons and iodo-hydrocarbons, with the majority being associated with insect cuticle metabolism. This study demonstrates that DI-SPME technology is an effective method for studying differentially expressed metabolites in T. castaneum with different levels of PH3 resistance. This approach may help to provide a better understanding of the development of insect PH3 resistance and act as a valuable reference for the establishment of rapid diagnostic techniques for insect PH3 resistance.
Molecular Toxicology and Cancer Prevention
Authors: Sun Guohui , Wang Chongwen
Published: November 2023

Molecular toxicology is a field that investigates the interactions between chemical or biological molecules and organisms at the molecular level [...]
Preanalytical Quality Evaluation of Citrate Evacuated Blood Collection Tubes—Ultraviolet Molecular Absorption Spectrometry Confronted with Ion Chromatography
Authors: Gros Nataša , Stopar Tjaša
Published: November 2023

We previously enabled a direct insight into the quality of citrate anticoagulant tubes before their intended use for specimen collection by introducing an easy-to-perform UV spectrometric method for citrate determination on a purified water model. The results revealed differences between the tubes of three producers, Greiner BIO-ONE (A), LT Burnik (B), and BD (C). It became apparent that tubes C contain an additive, which absorbs light in the ultraviolet range and prevents reliable evaluation of citrate anticoagulant concentration with the suggested method. In this research, we re-evaluate the quality of citrate-evacuated blood collection tubes by complementing UV spectrometry with ion chromatography. (1) Comparable results were obtained for tubes B at 220 nm. (2) Citrate concentrations determined with ion chromatography were lower for tubes A and C. Chromatograms reveal additional peaks for both. (3) Influences of heparin on absorption spectra and chromatograms of citrate were studied. Some similarities with the shape of the anticoagulant spectra of tubes A and C were observed, and the lithium heparin peak in chromatograms is close to them, but a confident judgment was not possible. (4) Contamination of anticoagulant solution with potassium, magnesium, and calcium was confirmed for all the brands, and contamination with lithium for B and C.
Influence of Amino Acid Substitutions in ApoMb on Different Stages of Unfolding of Amyloids
Authors: Katina Natalya , Marchenkov Victor , Ryabova Natalya , Ilyina Nelly , Marchenko Natalia , Balobanov Vitalii , Finkelstein Alexey
Published: November 2023

To date, most research on amyloid aggregation has focused on describing the structure of amyloids and the kinetics of their formation, while the conformational stability of fibrils remains insufficiently explored. The aim of this work was to investigate the effect of amino acid substitutions on the stability of apomyoglobin (ApoMb) amyloids. A study of the amyloid unfolding of ApoMb and its six mutant variants by urea has been carried out. Changes in the structural features of aggregates during unfolding were recorded by far-UV CD and native electrophoresis. It was shown that during the initial stage of denaturation, amyloids’ secondary structure partially unfolds. Then, the fibrils undergo dissociation and form intermediate aggregates weighing approximately 1 MDa, which at the last stage of unfolding decompose into 18 kDa monomeric unfolded molecules. The results of unfolding transitions suggest that the stability of the studied amyloids relative to the intermediate aggregates and of the latter relative to unfolded monomers is higher for ApoMb variants with substitutions that increase the hydrophobicity of the residues. The results presented provide a new insight into the mechanism of stabilization of protein aggregates and can serve as a base for further investigations of the amyloids’ stability.
Muslim Women Travelling Alone
Authors: Baykal Sahin
Published: November 2023

Whether women can travel alone has been debated for centuries in Islamic law. This article examines the Islamic legal principles concerning women travelling alone, whether it be for Hajj (pilgrimage to Mecca) or any journeys. Despite the explicit Quranic order about the duty of Hajj for all believers, depending upon the fulfilment of specific conditions, Sunni scholars have introduced additional criteria, particularly related to women, which have led to the establishment of gender-specific regulations. These interpretations are based on the ḥadīth of the Prophet rather than explicit verses from the Qur’an. The view that prevents women from travelling alone has gained dominance among Sunni scholars, and a maḥram (a male relative) becomes a requirement for a journey. However, Ibn Ḥazm of Cordoba (d. 1064) presents an opposing perspective that significantly differs from this consensus. Ibn Ḥazm believes that women can travel and participate in the Hajj without a maḥram, emphasising the importance of Hajj as a personal responsibility in terms of the religious obligation. This article provides a comprehensive analysis of the ḥadīth concerning women’s travel alone, the varying opinions of Sunni scholars, and the distinctive position adopted by Ibn Ḥazm. The text explains that Ibn Ḥazm’s analysis mainly based on a preference for reasonable arguments and egalitarian principles, prioritising them over literal interpretations of the ḥadīths regarding the topic.
Enhancing Resource Sharing and Access Control for VNF Instantiation with Blockchain
Authors: Dong Anwei , Wang Xingwei , Yi Bo , He Qiang , Huang Min
Published: November 2023

In the realm of Network Function Virtualization (NFV), Virtual Network Functions (VNFs) are crucial software entities that require execution on virtualized hardware infrastructure. Deploying a Service Function Chain (SFC) requires multiple steps for instantiating VNFs to analyze, request, deploy, and monitor resources. It is well recognized that the sharing of infrastructure resources among different VNFs will enhance resource utilization. However, conventional mechanisms for VNF sharing often neglect the interests of both VNF instances and infrastructure providers. In this context, this paper presents a blockchain-based framework that focuses on resource sharing and access control, with a particular emphasis on ensuring profitability during VNF instantiation. Additionally, a resource sharing game model and a novel greedy matching algorithm are introduced to optimize the benefits for both VNF instances and infrastructure resource providers. Furthermore, a blockchain-based access control mechanism is designed to securely store keys and provide fine-grained access control. The experimental results demonstrate that the proposed resource sharing game model and greedy matching algorithm promote healthy competition among resource owners and facilitate effective bargaining between resource owners and infrastructure providers. In comparison to the standard Stackelberg game solution, our proposed method achieves up to an 8.1 times performance improvement while sacrificing fewer optimal social utility values. Furthermore, compared to other CP-ABE methods, the proposed approach enhances security within a blockchain-based framework while maintaining an excellent encryption efficiency and a moderate decryption efficiency.
Image Segmentation of Fiducial Marks with Complex Backgrounds Based on the mARU-Net
Authors: Zhang Xuewei , Wang Jichun , Wang Yang , Feng Yanwu , Tang Shufeng
Published: November 2023

Circuits on different layers in a printed circuit board (PCB) must be aligned according to high-precision fiducial mark images during exposure processing. However, processing quality depends on the detection accuracy of fiducial marks. Precise segmentation of fiducial marks from images can significantly improve detection accuracy. Due to the complex background of PCB images, there are significant challenges in the segmentation and detection of fiducial mark images. In this paper, the mARU-Net is proposed for the image segmentation of fiducial marks with complex backgrounds to improve detection accuracy. Compared with some typical segmentation methods in customized datasets of fiducial marks, the mARU-Net demonstrates good segmentation accuracy. Experimental research shows that, compared with the original U-Net, the segmentation accuracy of the mARU-Net is improved by 3.015%, while the number of parameters and training times are not increased significantly. Furthermore, the centroid method is used to detect circles in segmentation results, and the deviation is kept within 30 microns, with higher detection efficiency. The detection accuracy of fiducial mark images meets the accuracy requirements of PCB production.
Multi-Stream Fusion Network for Skeleton-Based Construction Worker Action Recognition
Authors: Tian Yuanyuan , Liang Yan , Yang Haibin , Chen Jiayu
Published: November 2023

The global concern regarding the monitoring of construction workers’ activities necessitates an efficient means of continuous monitoring for timely action recognition at construction sites. This paper introduces a novel approach—the multi-scale graph strategy—to enhance feature extraction in complex networks. At the core of this strategy lies the multi-feature fusion network (MF-Net), which employs multiple scale graphs in distinct network streams to capture both local and global features of crucial joints. This approach extends beyond local relationships to encompass broader connections, including those between the head and foot, as well as interactions like those involving the head and neck. By integrating diverse scale graphs into distinct network streams, we effectively incorporate physically unrelated information, aiding in the extraction of vital local joint contour features. Furthermore, we introduce velocity and acceleration as temporal features, fusing them with spatial features to enhance informational efficacy and the model’s performance. Finally, efficiency-enhancing measures, such as a bottleneck structure and a branch-wise attention block, are implemented to optimize computational resources while enhancing feature discriminability. The significance of this paper lies in improving the management model of the construction industry, ultimately aiming to enhance the health and work efficiency of workers.
Low-Cost EEG Multi-Subject Recording Platform for the Assessment of Students’ Attention and the Estimation of Academic Performance in Secondary School
Authors: Fuentes-Martinez Victor Juan , Romero Samuel , Lopez-Gordo Miguel Angel , Minguillon Jesus , Rodríguez-Álvarez Manuel
Published: November 2023

The level of student attention in class greatly affects their academic performance. Teachers typically rely on visual inspection to react to students’ attention in time, but this subjective method leads to inconsistencies across classes. Online education exacerbates the issue as students can turn off cameras and microphones to keep their own privacy. To address this, we present a novel, low-cost EEG-based platform for assessing students’ attention and estimating their academic performance. In a study involving 34 secondary school students (aged 14 to 16), participants watched an academic video and answered evaluation questions while their EEG activity was recorded using a commercial headset. The results demonstrate a significant correlation (0.53, p-value = 0.003) between the power spectral density (PSD) of the EEG beta band (12–30 Hz) and students’ academic performance. Additionally, there was a notable difference in PSD-beta between high and low academic performers. These findings encourage the use of PSD-beta for the immediate and objective assessment of both the student attention and the subsequent academic performance. The platform offers valuable and objective feedback to teachers, enhancing the effectiveness of both face-to-face and online teaching and learning environments.
Analysis of Beneficial Effects of Flavonoids in Patients with Atherosclerosis Risk on Blood Pressure or Cholesterol during Random Controlled Trials: A Systematic Review and Meta-Analysis
Authors: Grijalva-Guiza Rosa Edith , Grijalva-Montano Thais Lucía , Cuautle Mariana , Quiroga-González Enrique , Hernández Luis Ricardo , Ortega Aguilar Alicia , Jiménez-Garduño Aura Matilde
Published: November 2023

Flavonoids are plant-secondary metabolites with cardiovascular protective properties. Few studies have examined specific flavonoid classes or pure flavonoids concerning some common cardiovascular risks. To obtain information in a systematic review to analyze in a meta-analysis, data were recovered regarding flavonoid intake in random controlled trials and atherosclerosis disease, related to risk factors such as blood pressure, total cholesterol (TC), and low-density lipoprotein cholesterol (LDLc). Our aim was to conduct a meta-analysis using the Scopus and PubMed databases without restrictions on the year of publication, extracting articles over the period 1–15 April 2023, searching for randomized controlled trials (RCTs) that investigated different types of flavonoids, measuring blood pressure and low-density cholesterol plasmatic concentration. This paper’s Prospero registration is CRD 42023414153. There were 19 RCTs: twelve RCTs were considered for blood pressure data analysis and fifteen RCTs for total cholesterol and LDL cholesterol data analysis. The meta-analysis showed no significant differences between placebo treatments and treatments with different flavonoids on blood pressure. However, there was a significant difference found in quantitative analysis for TC and LDLc. In conclusion, flavonoid consumption can be associated with a lower risk of LDLc and TC, and more RCTs are needed to specify the effect of more types of pure flavonoids in atherosclerotic patients.
Analysis of the state and development prospects of medicinal plant cultivation in The Republic of Belarus and Russia in the context of low-carbon agriculture
Authors:   Russian Timiryazev State Agrarian University, Moscow, Russia, Tryastsina N. Yu. , Dzhikуa М. К. ,   Russian Timiryazev State Agrarian University, Moscow, Russia, Karachevskaya. E. V. ,   Belarusian State Agricultural Academy of the Order of the October Revolution and the Red Banner of Labor, Gorki, Mogilev region, Republic of Belarus
Published: November 2023

The article is devoted to the analysis of one of the types of organic products — medicinal herbs in order to develop recommendations for improving this area of agricultural business in the Republic of Belarus and Russia. The dynamics of indicators characterizing medicinal plant growing is presented. A project has been proposed for the cultivation of medicinal herbs on the territory of private farms in the Republic of Belarus. The results of a study on the analysis of production indicators of medicinal crops in the context of regions of states are presented. Recommendations for the development of medicinal plant production in Russia and Belarus are proposed. The results obtained can be used to substantiate reserves for increasing the efficiency of medicinal plant production, to justify management decisions, as well as to develop a strategy for the sustainable development of agricultural enterprises.
Preparatory work for the formation of accounting and analytical support for managerial decision-making in agricultural organizations
Authors:   Russian Timiryazev State Agrarian University, Moscow, Russia, Romanova A. A. , Dzhikiya M. K. ,   Russian Timiryazev State Agrarian University, Moscow, Russia
Published: November 2023

The article provides a theoretical justification of approaches to the development of preparatory work in the formation of accounting and analytical support for managerial decision-making in agricultural organizations on the example of accounting in integrated structures. The forms of interaction between financial and managerial accounting for the purpose of improving the efficiency of decision-making management are disclosed. Adaptation of traditional tools for assessing management accounting technologies is the main task of preparatory work for the formation of accounting and analytical support for managerial decision–making in agricultural organization.
Penerapan E-Goverment Melalui Sistem Informasi Kesejahteraan Sosial Next Generation (SIKS-NG) Sebagai Aplikasi Pengolah Data Kemiskinan Di Kabupaten Bengkulu Selatan
Authors: Andriani Yevi , Suwitri Sri , Yuniningsih Tri
Published: November 2023

Penelitian ini bertujuan untuk mengevaluasi penerapan e-government melalui Sistem Informasi Kesejahteraan Sosial Next Generation (SIKS-NG) sebagai aplikasi pengolah data kemiskinan di Kabupaten Bengkulu Selatan. Dalam metodenya, peneliti menggunakan pendekatan kualitatif dengan metode deskriptif. Data dikumpulkan melalui observasi, wawancara, dan dokumentasi. Hasil penelitian menunjukkan bahwa dalam aspek dukungan, Kantor Dinas Sosial Kabupaten Bengkulu Selatan telah memberikan dukungan penuh terhadap SIKS-NG, meskipun masih terdapat kendala tertentu. Di sisi lain, dalam hal kapasitas, sumber daya finansial dan infrastruktur teknologi masih belum memadai, dan kekurangan tenaga ahli di bidang IT menjadi hambatan. Namun, manfaat dari SIKS-NG sudah terasa, mempermudah masyarakat dalam mendapatkan informasi dan mengelola data bantuan sosial, walaupun masih ada masyarakat miskin yang belum terdata.