bluuebunny/crossref_metadata_2025 huggingface dataset:
Case report: Brief, intensive EMDR therapy for borderline personality disorder: results of two case studies with one year follow-up
Authors: Hafkemeijer Laurian , Slotema Karin , Haard Nicole de , Jongh Ad de
Published: December 2023

Background

Exposure to adverse childhood events plays an important role in the development of borderline personality disorder (BPD). Emerging evidence suggests that trauma-focused therapy using eye movement desensitization and reprocessing (EMDR) can be beneficial for patients with BPD symptoms. To date, the effects of brief, intensive EMDR treatment for this target group have not been investigated in this population.

Objective

This study aimed to evaluate the effects of a brief and intensive trauma-focused therapy course using EMDR therapy in two patients diagnosed with BPD who did not fulfill the diagnostic criteria for post-traumatic stress disorder (PTSD). It was hypothesized that this approach would be associated with a decline in the core symptoms of BPD, and that this would have an enduring long-term effect on patients’ diagnostic status.

Method

Ten sessions of EMDR therapy were carried out across four consecutive treatment days, with the aim of processing patients’ core adverse childhood experiences. Both A-criterion-worthy memories (without intrusive reliving) and non-A-criterion-worthy memories that were considered responsible for the patients’ most prominent symptoms were targeted. The effects of EMDR therapy on trauma symptom severity and BPD diagnostic status (as established by the Structured Clinical Interview DSM-5) were determined. Additionally, the effects on psychological distress, quality of life, and difficulties in emotion regulation were determined at intake, post-treatment, and at 3-, 6-, and 12-months follow-up.

Results

Both patients showed a strong decline in psychological distress and difficulties in emotion regulation, and reported an improvement in their quality of life. At post-treatment, and at 3-, 6-, and 12-months follow-up they no longer met the DSM-5 criteria for BPD.

Conclusion

The findings of this small case study are in line with mounting evidence that a brief track of intensive trauma-focused therapy can result in long-term remission in patients with BPD. EMDR therapy seems to be a promising treatment approach for patients with BPD; however, the results need to be replicated in clinical trials.
Factors influencing the likelihood of dental service checkup: results from a survey in Saudi Arabia
Authors: Almutairi Majed , McKenna Gerry , Alsumaih Ibrahim , Alhazzaa Rasha , O’Neill Ciaran
Published: December 2023

Background

The funding and delivery of healthcare including dental care in the Kingdom of Saudi Arabia (KSA, or Saudi Arabia) is undergoing a process of reform. To inform this process, it is important that policymakers are aware of the relationships between service use, specific types of use, and the factors that influence this. Currently, there is a paucity of research in this area in KSA that examines dental service use for checkups at a national level and none that examines differences in this use across regions or that examines explicitly the role of income.

Aims

This study uses the most recent version of the Saudi Health Interview Survey (SHIS) to examine the relationships between the use of dental services for a checkup and socio-demographic characteristics of respondents. Particular focus is given to the differences between regions in service use and the role of socio-demographics within regions.

Methods

Data were taken from SHIS 2013. Descriptive statistics (means and standard errors) were used to characterize the sample. Logistic regression analyses were used to examine the relationship between checkups in the past 12 months and a range of covariates including income and region. The analysis was repeated for sub-samples based on specific regions. No attempt was made to impute missing values.

Results

A sample of 7603 respondents provided complete data for analysis. Fifty-one per cent of the respondents were male, 29% were educated at least to degree level, 25% reported that they floss at least once per day, 69% reported that they brushed their teeth at least once per day, and 11% reported that they had visited the dentist for a checkup in the preceding 12 months. Logistic regression analyses revealed income, region, and oral hygiene habits to be among the significant determinants of the likelihood of dental checkup in the preceding 12 months. In logistic regression analyses at the regional level, different relationships were evident between checkups and socio-demographic characteristics across regions.

Conclusion

Region and income are significant determinants of dental service use for checkups. Differences exist between regions in the relationship between socio-demographic characteristics and the likelihood of getting checkups. Policy changes should reflect the potential differences they might have across regions for which the role of socio-demographic characteristics varies.
Novel embryo selection strategies—finding the right balance
Authors: Polyakov Alex , Rozen Genia , Gyngell Chris , Savulescu Julian
Published: December 2023

The use of novel technologies in the selection of embryos during in vitro fertilisation (IVF) has the potential to improve the chances of pregnancy and birth of a healthy child. However, it is important to be aware of the potential risks and unintended consequences that may arise from the premature implementation of these technologies. This article discusses the ethical considerations surrounding the use of novel embryo selection technologies in IVF, including the growing uptake of genetic testing and others, and argues that prioritising embryos for transfer using these technologies is acceptable, but discarding embryos based on unproven advances is not. Several historical examples are provided, which demonstrate possible harms, where the overall chance of pregnancy may have been reduced, and some patients may have missed out on biological parenthood altogether. We emphasise the need for caution and a balanced approach to ensure that the benefits of these technologies outweigh any potential harm. We also highlight the primacy of patients' autonomy in reproductive decision-making, especially when information gained by utilising novel technologies is imprecise.
Un-settling the frontiers of food waste in the Netherlands: infrastructural stories of food waste transformation in the Hague
Authors: Vicherat Mattar Daniela , Thrivikraman Jyothi , Burgos Martínez Elena
Published: December 2023

Transformation opens possibilities to imagine and learn other forms of being and living together; it implies change, and often exchange. This paper visibilises the infrastructural stories involved in the transformation of food waste into edible food. Conceptually, we assume there is no univocal definition of food waste. Methodologically, we resist the practice of extracting and appropriating the life (hi)stories of the people involved in these transformative initiatives who are often described as vulnerable “others.” Instead, the project highlights the stories of the infrastructures underpinning these transformative experiences. The objective is to unsettle the frontier of what counts as food waste by paying attention to the objects involved in the process of transforming it into edible food. The paper starts by positioning ourselves in the literature regarding food waste governance and infrastructural labor as transformative possibilities that support the development of personal sustainability strategies and grassroots food governance. In the second section we explain our methodological considerations to focus on the infrastructures that mediate the transformation of disposed food into edible food in The Hague. The third section presents the results and discusses the stories of two objects, a cargo bike (bakfiets) and a fridge (koelkast). We suggest these objects serve to unsettle governance practices and narratives related to food waste in the city. Paying attention to these objects’ stories is an important turn in personal sustainability research because it enables us to see that the individual is never alone, always acting with the support of often invisible objects that in this study make possible the reduction and transformation of wasted food. This serves as inspiration to imagine alternative paths for food waste governance and foster forms of collective engagement, urgently needed given the increasing precariousness of food systems and relations.
Challenges and proposed solutions for optical reading on point-of-need testing systems
Authors: Schaumburg Federico , Berli Claudio L. A.
Published: December 2023

A broad spectrum of application fields, including human and animal health, food safety and environmental monitoring, require performing real time, on-field chemical determinations, a concept known as point-of-need testing (PONT). In the case of infectious diseases, it allows for early diagnosis, which usually enables better treatment options for the patient, and permits early outbreak detection and intervention with proper control measures. PONT solutions are usually developed on microfluidic chips, preferably with no extra equipment, as to lower their cost, ease their delivery and portability. However, equipment-free PONT is not always feasible, since off-chip operations (e.g., pumping or heating) might be required and provided by the so-called analyzers. A typical example is the hardware for optical reading, which is the subject of this perspective article. Firstly, the state-of-the-art is briefly analyzed considering the solutions provided by both academy and industry. Special emphasis is made on the smartphone-based approaches that use no additional hardware (add-ons) for optical reading. Smartphones are the straightforward option, since they can replace a number of operations typically done by analyzers. The issues arising from using smartphones without add-ons are classified into variability sources and technical challenges. Finally, a set of methods for dealing with these challenges is proposed and briefly discussed. Simpler systems will be more easily adopted by more users, for a broader range of application cases, and will let PONT be a more powerful tool for improving disease diagnosis and outbreak management.
Precision public health in the making: examining the becoming of the “social” in a Swiss environmental health population-based cohort
Authors: Bühler Nolwenn
Published: December 2023

Expanding the concept of “precision” or “personalized” medicine, personalized health and precision public health designate the use of various kinds of data—genomic, other omics, clinical, or those produced by individuals themselves through self-tracking—to optimize health interventions benefiting the whole population. This paper draws on an ethnography of the implementation of a population-based environmental health cohort to shed light on the reconfigurations brought about by the “personalization” of public health in Switzerland. Combining human biomonitoring and molecular epidemiology, this cohort aims to advance the science of the exposome, a notion referring to the totality of exposures to which individuals are subjected over their lifecourse. Addressing the tension between holism and reductionism, this paper points to the important gap between the promissory horizon of the exposome and the realities of practices. Situations of reductionism are defined as moments of friction and negotiation between different rationales and values, exposing what makes the science of the exposome, including its material, economic, institutional, and methodological constraints, as well as its imaginaries and values. Rather than opposing holism and reductionism, I emphasize that they constitute two sides of the same coin, as they both pragmatically enable action and produce situated versions of the social. This empirical case shows how reductionism operates at the chemical, biological, and populational levels to produce public health scientific and social values. It thus contributes to contextualizing the pragmatic and strategic choices made by scientists, as well as the values they favor, in a research environment marked by the predominance of biomedicine over public health. It shows how the reductionism of the “social environment” was made for a better social integration of the cohort into the Swiss political and scientific landscape of public health. Bringing together actors involved in public health and questions of environmental exposures, this cohort can be interpreted as a biomedicalization of public health research, as well as an attempt to socialize it through the broad category of the exposome.
Impact of high-standard farmland construction on farmers’ income growth—quasi-natural experiments from China
Authors: Yusheng Chen , Zhaofa Sun , Yanmei Wang , Yang Han
Published: December 2023

This paper examines the impact of high-standard basic farmland construction on farmers’ income in China using panel data at the provincial level from 2006 to 2021. The study aims to provide insights into the relationship between high-standard basic farmland construction and farmers’ income by employing a difference-in-differences model. The research background of this study is rooted in the importance of agricultural development and rural income improvement in China. As the agricultural sector plays a crucial role in ensuring food security and rural stability, it is essential to explore the effects of high-standard basic farmland construction on farmers’ income. The primary objective of this research is to estimate the impact of high-standard basic farmland construction on farmers’ income and to identify any heterogeneity in this relationship across different regions and income levels. By analyzing the baseline regression results, the study finds a significant positive effect of high-standard basic farmland construction on farmers’ income. To gain a deeper understanding of the mechanisms underlying this relationship, the study conducts further analysis on the impact pathways. The findings suggest that high-standard basic farmland construction enhances farmers’ income through improvements in agricultural production conditions, land transfer levels, and the cultivation of new agricultural management entities. Furthermore, the study explores the role of high-standard basic farmland construction in grain-producing and non-grain-producing regions. In grain-producing regions, high-standard basic farmland construction primarily increases farmers’ income from family-operated businesses. Conversely, in non-grain-producing regions, it predominantly boosts farmers’ income from wages and property. Overall, this research contributes to the understanding of the impact of high-standard basic farmland construction on farmers’ income in China. The findings highlight the importance of promoting high-standard basic farmland construction for rural income improvement, with implications for agricultural policies and rural development strategies.
Carpal tunnel syndrome caused by tophi in the superficial flexor tendon: a case report
Authors: Hao Haihu , Kong Weijie , Li Han
Published: December 2023

Carpal tunnel syndrome (CTS) is the most common disease among peripheral nerve entrapment diseases. CTS is often caused by the hyperplasia of the transverse carpal ligament and edema of tissue in the carpal tunnel, resulting in compression of the median nerve. Specific manifestations of CTS include numbness, loss of skin sensation in the palm and three and a half fingers on the radial side, and decreased muscle strength; however, CTS caused by wrist tophi is very rare. To our knowledge, CTS with median nerve compression caused by tophi in the superficial flexor tendon of the index finger of the wrist has not been reported before. Here, we will report a case of CTS caused by tophi in the wrist in a 37-year-old patient with no history of gout. CTS caused by tophi is uncommon, but if the patient has high uric acid, CTS may be due to tophi.
Clinical and radiological analysis of the effects of three different lumbar transpedicular dynamic stabilization system on disc degeneration and regeneration
Authors: Karadag Mehmet Kursat , Akgun Mehmet Yigit , Basak Ahmet Tulgar , Ates Ozkan , Tepebasili Mehmet Ali , Gunerbuyuk Caner , Oktenoglu Tunc , Sasani Mehdi , Ozer Ali Fahir
Published: December 2023

Objective

This study aims to assess the clinical outcomes of three transpedicular dynamic systems in treating degenerative disc disease and evaluate their impact on both clinical and radiological aspects of the operated and adjacent segments.

Materials and methods

A total of 111 patients who underwent posterior transpedicular short-segment dynamic system procedures for treatment of degenerative disc disease were included. The patients were categorized into three groups, namely, Group 1 (Dynesys system, n = 38), Group 2 (Safinaz screw + PEEK rod, n = 37), and Group 3 (Safinaz screw + titanium rod, n = 36). Disc regeneration in the operated segment and disc degeneration in the operated, upper, and lower adjacent segments were assessed using the Pfirrmann Classification.

Results

Postoperatively, a statistically significant difference was observed in visual analog scale and Oswestry Disability Index scores (p < 0.001). However, no statistically significant difference was seen in disc degeneration/regeneration and degeneration scores of the upper and lower adjacent segments between the preoperative and postoperative groups (p = 0.763, p = 0.518, p = 0.201). Notably, a positive effect on disc regeneration at the operated level (32.4%) was observed. No significant differences were found between the groups in terms of operation rates, screw loosening, and screw breakage after adjacent segment disease (p > 0.05).

Conclusion

In patients without advanced degeneration, all three dynamic systems demonstrated the ability to prevent degeneration in the adjacent and operated segments while promoting regeneration in the operated segment. Beyond inhibiting abnormal movement in painful segments, maintaining physiological motion and providing axial distraction in the operated segment emerged as key mechanisms supporting regeneration.
A Cross-Sectional Study of the Prevalence and Determinants of Common Mental Health Problems in Primary Care in Switzerland
Authors: Messer Juliane , Tzartzas Konstantinos , Marion-Veyron Régis , Cohidon Christine
Published: December 2023

Objective: This study investigated the prevalence of the most common mental health symptoms in a large primary care patient population and characterized their determinants.

Methods: Data came from a 2015–16 cross-sectional study of a primary care population in Switzerland. An investigator presented the study to patients in waiting rooms, and 1,103 completed a tablet-based questionnaire measuring stress in daily life, sleep disorders and anxiety and depressive symptoms. Diagnoses and treatments were recorded.

Results: Moderate-to-high anxiety and depressive symptoms concerned 7.7% of patients; 27.6% felt stressed at least once a week; 17.2% had severe sleep disorders. Sociodemographic determinants were associated with psychiatric symptoms: female sex, young age, and frequency of consultations with a GP. Participants taking psychotropics had high levels of mental distress.

Conclusion: Even though most patients were regularly monitored by their GP, a significant number of mental health problems were found. GPs should be provided with concrete tools to manage these patients better. Collaboration with mental health specialists should be encouraged in primary care settings.
Deep Learning-Based Visual Complexity Analysis of Electroencephalography Time-Frequency Images: Can It Localize the Epileptogenic Zone in the Brain?
Authors: Makaram Navaneethakrishna , Gupta Sarvagya , Pesce Matthew , Bolton Jeffrey , Stone Scellig , Haehn Daniel , Pomplun Marc , Papadelis Christos , Pearl Phillip , Rotenberg Alexander , Grant Patricia Ellen , Tamilia Eleonora
Published: December 2023

In drug-resistant epilepsy, a visual inspection of intracranial electroencephalography (iEEG) signals is often needed to localize the epileptogenic zone (EZ) and guide neurosurgery. The visual assessment of iEEG time-frequency (TF) images is an alternative to signal inspection, but subtle variations may escape the human eye. Here, we propose a deep learning-based metric of visual complexity to interpret TF images extracted from iEEG data and aim to assess its ability to identify the EZ in the brain. We analyzed interictal iEEG data from 1928 contacts recorded from 20 children with drug-resistant epilepsy who became seizure-free after neurosurgery. We localized each iEEG contact in the MRI, created TF images (1–70 Hz) for each contact, and used a pre-trained VGG16 network to measure their visual complexity by extracting unsupervised activation energy (UAE) from 13 convolutional layers. We identified points of interest in the brain using the UAE values via patient- and layer-specific thresholds (based on extreme value distribution) and using a support vector machine classifier. Results show that contacts inside the seizure onset zone exhibit lower UAE than outside, with larger differences in deep layers (L10, L12, and L13: p < 0.001). Furthermore, the points of interest identified using the support vector machine, localized the EZ with 7 mm accuracy. In conclusion, we presented a pre-surgical computerized tool that facilitates the EZ localization in the patient’s MRI without requiring long-term iEEG inspection.
The Solar Particle Acceleration Radiation and Kinetics (SPARK) Mission Concept
Authors: Reid Hamish A. S. , Musset Sophie , Ryan Daniel F. , Andretta Vincenzo , Auchère Frédéric , Baker Deborah , Benvenuto Federico , Browning Philippa , Buchlin Éric , Calcines Rosario Ariadna , Christe Steven D. , Corso Alain Jody , Dahlin Joel , Dalla Silvia , Del Zanna Giulio , Denker Carsten , Dudík Jaroslav , Erdélyi Robertus , Ermolli Ilaria , Fletcher Lyndsay , Fludra Andrzej , Green Lucie M. , Gordovskyy Mykola , Guglielmino Salvo L. , Hannah Iain , Harrison Richard , Hayes Laura A. , Inglis Andrew R. , Jeffrey Natasha L. S. , Kašparová Jana , Kerr Graham S. , Kintziger Christian , Kontar Eduard P. , Krucker Säm , Laitinen Timo , Laurent Philippe , Limousin Olivier , Long David M. , Maloney Shane A. , Massa Paolo , Massone Anna Maria , Matthews Sarah , Mrozek Tomasz , Nakariakov Valery M. , Parenti Susanna , Piana Michele , Polito Vanessa , Pesce-Rollins Melissa , Romano Paolo , Rouillard Alexis P. , Sasso Clementina , Shih Albert Y. , Stęślicki Marek , Orozco Suárez David , Teriaca Luca , Verma Meetu , Veronig Astrid M. , Vilmer Nicole , Vocks Christian , Warmuth Alexander
Published: December 2023

Particle acceleration is a fundamental process arising in many astrophysical objects, including active galactic nuclei, black holes, neutron stars, gamma-ray bursts, accretion disks, solar and stellar coronae, and planetary magnetospheres. Its ubiquity means energetic particles permeate the Universe and influence the conditions for the emergence and continuation of life. In our solar system, the Sun is the most energetic particle accelerator, and its proximity makes it a unique laboratory in which to explore astrophysical particle acceleration. However, despite its importance, the physics underlying solar particle acceleration remain poorly understood. The SPARK mission will reveal new discoveries about particle acceleration through a uniquely powerful and complete combination of γ-ray, X-ray, and EUV imaging and spectroscopy at high spectral, spatial, and temporal resolutions. SPARK’s instruments will provide a step change in observational capability, enabling fundamental breakthroughs in our understanding of solar particle acceleration and the phenomena associated with it, such as the evolution of solar eruptive events. By providing essential diagnostics of the processes that drive the onset and evolution of solar flares and coronal mass ejections, SPARK will elucidate the underlying physics of space weather events that can damage satellites and power grids, disrupt telecommunications and GPS navigation, and endanger astronauts in space. The prediction of such events and the mitigation of their potential impacts are crucial in protecting our terrestrial and space-based infrastructure.
Assessment of a Novel Real-Time Bio-Liquor Circulation System for Manure Management and Mitigation of Odor Potential in Swine Farming
Authors: Kim Seungsoo , Shim Soomin , Won Seunggun , Ra Changsix
Published: December 2023

Recently, circulating biologically treated manure in slurry pits has been used as an odor reduction technology, but few successful results have been reported, due to the lack of proper control strategies for bioreactors. This study was conducted to investigate the performance of the developed real-time controlled bio-liquor circulation system (BCS) at farm scale. The BCS was operated sequentially as per swine manure inflow (anoxic, aerobic, and settling) circulation to the slurry pit. Each operational phase was self-adjusted in real-time using a novel algorithm for detecting the control point on the oxidation reduction potential (ORP) and pH (mV)–time profiles, the nitrogen break point (NBP), and the nitrate knee point (NKP) in the aerobic and anoxic phases, respectively. The NH4-N in the slurry manure was thoroughly removed (100%) in the bioreactor, optimizing the duration of each operational phase by accurately detecting real-time control points. The newly developed real-time BCS decreased the nitrogen and organic matter in the slurry pit by >70%, and the potential ammonia and methane emissions by 75% and 95%, respectively. This study highlights that improved BCS that utilizes ORP tracking and pH (mV)–time profiles can effectively optimize BCS operation, and thereby reduce malodor and GHG emissions from swine farms.
Comparative Serum Proteome Profiling of Canine Benign Prostatic Hyperplasia before and after Castration
Authors: Ploypetch Sekkarin , Wongbandue Grisnarong , Roytrakul Sittiruk , Phaonakrop Narumon , Prapaiwan Nawarus
Published: December 2023

BPH is the most prevalent prostatic condition in aging dogs. Nevertheless, clinical diagnosis and management remain inconsistent. This study employed in-solution digestion coupled with nano-liquid chromatography tandem mass spectrometry to assess serum proteome profiling of dogs with BPH and those dogs after castration. Male dogs were divided into two groups; control and BPH groups. In the BPH group, each dog was evaluated at two time points: Day 0 (BF subgroup) and Day 30 after castration (AT subgroup). In the BF subgroup, three proteins were significantly upregulated and associated with dihydrotestosterone: solute carrier family 5 member 5, tyrosine-protein kinase, and FRAT regulator of WNT signaling pathway 1. Additionally, the overexpression of polymeric immunoglobulin receptors in the BF subgroup hints at its potential as a novel protein linked to the BPH development process. Conversely, alpha-1-B glycoprotein (A1BG) displayed significant downregulation in the BF subgroup, suggesting A1BG’s potential as a predictive protein for canine BPH. Finasteride was associated with increased proteins in the AT subgroup, including apolipoprotein C-I, apolipoprotein E, apolipoprotein A-II, TAO kinase 1, DnaJ homolog subfamily C member 16, PH domain and leucine-rich repeat protein phosphatase 1, neuregulin 1, and pseudopodium enriched atypical kinase 1. In conclusion, this pilot study highlighted alterations in various serum proteins in canine BPH, reflecting different pathological changes occurring in this condition. These proteins could be a source of potential non-invasive biomarkers for diagnosing this disease.
Polymorphism of the MTNR1A Melatonin Receptor Gene in Goat Breeds of Northern Italy
Authors: Agradi Stella , Luridiana Sebastiano , Mura Maria Consuelo , Cosso Giovanni , Vigo Daniele , Menchetti Laura , Brecchia Gabriele , Carcangiu Vincenzo , Curone Giulio
Published: December 2023

Melatonin receptor MT1, encoded by the MTNR1A gene, is the main one involved in the seasonal regulation of reproductive activity. A correlation between this gene polymorphism and reproductive performance has been demonstrated in sheep. To date, no data about MTNR1A gene polymorphism are available regarding Italian goat breeds other than the Sarda goat. This study aimed to detect any PCR-RFLP polymorphic sites of MTNR1A using MnlI and RsaI enzymes in Northern Italian goat breeds, which are characterized by a pronounced reproductive seasonality. One-hundred-eight adult female goats belonging to four different breeds were included in the study (i.e., Frisa Valtellinese, n = 29; Orobica, n = 23; Lariana, n = 29; Camosciata delle Alpi, n = 27). Blood was sampled from each goat. Genomic DNA was extracted from each sample and the main part of exon II of MTNR1A gene was amplified by PCR and digested with MnlI and RsaI enzymes. Unexpectedly, none of the fragments were found to be polymorphic. The absence of polymorphism may be linked to the macro group of goat breeds that evolved during human migrations. Breeds of the Alpine–European strain would appear to show no polymorphism, as confirmed by our study, whereas breeds belonging to the Mediterranean–African or Asian–Middle Eastern strains do.
Age-Related Changes in Hepatic Lipid Metabolism and Abdominal Adipose Deposition in Yellow-Feathered Broilers Aged from 1 to 56 Days
Authors: Lan Ruixia , Wei Linlin , Yu Haibin , Jiang Ping , Zhao Zhihui
Published: December 2023

The objective of this study was to evaluate the age-related changes in hepatic lipid metabolism, adipocyte hyperplasia, hypertrophy, and lipid metabolism in the abdominal adipose tissue of yellow-feathered broilers. Blood, liver, and abdominal adipose samples were collected on days 1, 7, 14, 21, 28, 35, 42, 49, and 56. Body, liver, and abdominal weight increased (p < 0.05) with age-related changes. The triacylglycerol content peaked on day 14, and total cholesterol content peaked on day 56. The adipocyte diameter and area peaked on day 56, and total DNA content peaked on day 7. The age-related changes in hepatic lipogenesis-related gene (ChREBP, SREBP-1c, ACC, FAS, SCD1) expression mainly occurred during days 1 to 21, hepatic lipolysis-related gene (CPT1, LPL, ApoB) expression mainly occurred during days 1 to 14, and abdominal adipose-deposition-related gene (PPARα, CPT1, LPL, PPARγ, C/EBPβ) expression occurred during days 1 to 14. These results demonstrated a dynamic pattern of hepatic lipid metabolism and abdominal adipose deposition in yellow-feathered broilers, which provides practical strategies to regulate hepatic lipid metabolism and reduce abdominal adipose deposition in yellow-feathered broilers.
Fostering Animal Welfare and Advancing 3Rs Principles through the Establishment of a 3Rs Advisory Group
Authors: Graham Jessica C. , Wong Lisa , Adedeji Adeyemi O. , Kusi Aija , Lee Becky , Lee Donna , Dybdal Noel
Published: December 2023

Based on the current state of science, the use of animals remains essential in bringing safe and effective medicines to patients. Respect for laboratory animal welfare and the application of 3Rs principles (the replacement, reduction, and refinement of animal use in research) are a priority throughout the pharmaceutical industry. Given the rapid pace of development, technological progress, and the emergence of new-approach methodologies (NAMs) in the field of biomedical research, maintaining a leading position in scientific advancements with a focus on the principles of replace, reduce, and refine (3Rs) can be quite challenging. To effectively address these challenges and sustain a prominent position in the scientific community, organizations can derive significant advantages from establishing an internal 3Rs advisory group (3Rs AG). The primary objective of a 3Rs AG is to stay at the forefront of the knowledge of best practices related to the 3Rs principles in the industry. This group plays a crucial role in fostering innovation and facilitating the seamless integration and implementation of 3Rs principles into a company’s policies and procedures. The thoughtful reduction in and replacement of animal studies and the refinement of study designs and practices, enabled by a 3Rs AG, can minimize animal use as well as guide resources and positively impact study and data quality. This article provides guidance on how to establish a successful and impactful 3Rs AG.
Assessment of Radon and Naturally Occurring Radionuclides in the Vredefort Meteorite Crater in South Africa
Authors: Le Roux Rikus , Bezuidenhout Jacques
Published: December 2023

The concentric impact rings of the Vredefort Crater contain rocks with elevated uranium concentrations resulting from the geological signature of a meteoric impact. The decay of this uranium was estimated to lead to elevated indoor radon concentrations in the Crater, but such a study has never been carried out. This study explores the relationship between the natural radionuclides found in the geology of the Vredefort Crater and indoor radon concentrations. This was achieved through soil sampling and radionuclide surveys conducted on three impact rings, supplemented by indoor radon measurements in dwellings found in the area. In situ measurements revealed that one impact ring had higher-than-average uranium concentrations at 50 Bq/kg. Surprisingly, the measured indoor radon levels were lower than expected (113 Bq/m3). These measurements were taken during the COVID-19 pandemic and colder months, conditions that would typically result in elevated indoor radon levels. Soil samples indicated uranium activity of 30 Bq/kg, comparable to the world average of 35 Bq/kg. However, defunct mine tunnels in the area exhibited elevated radon concentrations, averaging 364 Bq/m3. The disparity between expected and measured indoor radon levels was attributed to the composition of surficial deposits, bedrock, and architectural features of the dwellings preventing radon accumulation.
Anti-Apoptosis Therapy for Meniscal Avascular Zone Repair: A Proof-of-Concept Study in a Lapine Model
Authors: Yan Wenqiang , Wu Yue , Zhao Fengyuan , Dai Ruilan , Zhou Yunan , Liu Dingge , Cheng Jin , Hu Xiaoqing , Ao Yingfang
Published: December 2023

In the present study, 24 rabbits were firstly used to evaluate the apoptosis index and matrix degeneration after untreated adult meniscal tears. Vertical tears (0.25 cm in length) were prepared in the avascular zone of the anterior horn. Specimens were harvested at 1, 3, 6, 12 weeks postoperatively. The apoptosis index around tear sites stayed at a high level throughout the whole follow-up period. The depletion of glycosaminoglycans (GAG) and aggrecan at the tear site was observed, while the deposition of COL I and COL II was not affected, even at the last follow-up of 12 weeks after operation. The expression of SOX9 decreased significantly; no cellularity was observed at the wound interface at all timepoints. Secondly, another 20 rabbits were included to evaluate the effects of anti-apoptosis therapy on rescuing meniscal cells and enhancing meniscus repair. Longitudinal vertical tears (0.5 cm in length) were made in the meniscal avascular body. Tears were repaired by the inside-out suture technique, or repaired with sutures in addition to fibrin gel and blank silica nanoparticles, or silica nanoparticles encapsulating apoptosis inhibitors (z-vad-fmk). Samples were harvested at 12 months postoperatively. We found the locally administered z-vad-fmk agent at the wound interface significantly alleviated meniscal cell apoptosis and matrix degradation, and enhanced meniscal repair in the avascular zone at 12 months after operation. Thus, local administration of caspase inhibitors (z-vad-fmk) is a promising therapeutic strategy for alleviating meniscal cell loss and enhancing meniscal repair after adult meniscal tears in the avascular zone.
Application of a Radiomics Machine Learning Model for Differentiating Aldosterone-Producing Adenoma from Non-Functioning Adrenal Adenoma
Authors: Yang Wenhua , Hao Yonghong , Mu Ketao , Li Jianjun , Tao Zihui , Ma Delin , Xu Anhui
Published: December 2023

To evaluate the secretory function of adrenal incidentaloma, this study explored the usefulness of a contrast-enhanced computed tomography (CECT)-based radiomics model for distinguishing aldosterone-producing adenoma (APA) from non-functioning adrenal adenoma (NAA). Overall, 68 APA and 60 NAA patients were randomly assigned (8:2 ratio) to either a training or a test cohort. In the training cohort, univariate and least absolute shrinkage and selection operator regression analyses were conducted to select the significant features. A logistic regression machine learning (ML) model was then constructed based on the radiomics score and clinical features. Model effectiveness was evaluated according to the receiver operating characteristic, accuracy, sensitivity, specificity, F1 score, calibration plots, and decision curve analysis. In the test cohort, the area under the curve (AUC) of the Radscore model was 0.869 [95% confidence interval (CI), 0.734–1.000], and the accuracy, sensitivity, specificity, and F1 score were 0.731, 1.000, 0.583, and 0.900, respectively. The Clinic–Radscore model had an AUC of 0.994 [95% CI, 0.978–1.000], and the accuracy, sensitivity, specificity, and F1 score values were 0.962, 0.929, 1.000, and 0.931, respectively. In conclusion, the CECT-based radiomics and clinical radiomics ML model exhibited good diagnostic efficacy in differentiating APAs from NAAs; this non-invasive, cost-effective, and efficient method is important for the management of adrenal incidentaloma.
Revolutionizing Patient Monitoring in Age-Related Macular Degeneration: A Comparative Study on the Necessity and Efficiency of the AMD VIEWER
Authors: Tabuchi Hitoshi , Yamauchi Tomofusa , Nagasawa Toshihiko , Deguchi Hodaka , Tanabe Mao , Tanaka Hayato , Yasukawa Tsutomu
Published: December 2023

(1) Background: Age-related Macular Degeneration (AMD) is a critical condition leading to blindness, necessitating lifelong clinic visits for management, albeit with existing challenges in monitoring its long-term progression. This study introduced and assessed an innovative tool, the AMD long-term Information Viewer (AMD VIEWER), designed to offer a comprehensive display of crucial medical data—including visual acuity, central retinal thickness, macular volume, vitreous injection treatment history, and Optical Coherent Tomography (OCT) images—across an individual eye’s entire treatment course. (2) Methods: By analyzing visit frequencies of patients with a history of invasive AMD treatment, a comparative examination between a Dropout group and an Active group underscored the clinical importance of regular visits, particularly highlighting better treatment outcomes and maintained visual acuity in the Active group. (3) Results: The efficiency of AMD VIEWER was proven by comparing it to manual data input by optometrists, showing significantly faster data display with no errors, unlike the time-consuming and error-prone manual entries. Furthermore, an elicited Net Promoter Score (NPS) of 70 from 10 ophthalmologists strongly endorsed AMD VIEWER’s practical utility. (4) Conclusions: This study underscores the importance of regular clinic visits for AMD patients. It suggests the AMD VIEWER as an effective tool for improving treatment data management and display.
Post-Operative Delirium and Cognitive Dysfunction in Aged Patients Undergoing Cardiac Surgery: A Randomized Comparison between Two Blood Oxygenators
Authors: Mantovani Lorenzo , Mikus Elisa , Tenti Elena , Sangiorgi Diego , Zannoni Samantha , Cavallucci Andrea , Ferroni Letizia , Cimaglia Paolo , Tolio Valentina , Tremoli Elena , Savini Carlo
Published: December 2023

In elderly patients undergoing cardiac surgery, extracorporeal circulation affects the incidence of post-operative delirium and cognitive impairment with an impact on quality of life and mortality. In this study, a new oxygenator system (RemoweLL 2) was tested against a conventional system to assess its efficacy in reducing the onset of postoperative delirium and cognitive dysfunction and the levels of serum inflammatory markers. A total of 154 patients (>65 y.o.) undergoing cardiopulmonary bypass (CPB) were enrolled and randomly assigned to oxygenator RemoweLL 2 (n = 81) or to gold standard device Inspire (n = 73) between September 2019 and March 2022. The aims of the study were to assess the incidence of delirium and the cognitive decline by neuropsychiatric tests and the MoCa test intra-hospital and at 6 months after CPB. Inflammation biomarkers in both groups were also evaluated. Before the CPB, the experimental groups were comparable for all variables. After CPB, the incidence of severe post-operative delirium showed a better trend (p = 0.093) in patients assigned to RemoweLL 2 (16.0%) versus Inspire (26.0%). Differences in enolase levels (p = 0.049), white blood cells (p = 0.006), and neutrophils (p = 0.003) in favor of RemoweLL 2 were also found. The use of novel and better construction technologies in CPB oxygenator devices results in measurable better neurocognitive and neurological outcomes in the elderly population undergoing CPB.
Enhancing Skin Lesion Detection: A Multistage Multiclass Convolutional Neural Network-Based Framework
Authors: Ali Muhammad Umair , Khalid Majdi , Alshanbari Hanan , Zafar Amad , Lee Seung Won
Published: December 2023

The early identification and treatment of various dermatological conditions depend on the detection of skin lesions. Due to advancements in computer-aided diagnosis and machine learning approaches, learning-based skin lesion analysis methods have attracted much interest recently. Employing the concept of transfer learning, this research proposes a deep convolutional neural network (CNN)-based multistage and multiclass framework to categorize seven types of skin lesions. In the first stage, a CNN model was developed to classify skin lesion images into two classes, namely benign and malignant. In the second stage, the model was then used with the transfer learning concept to further categorize benign lesions into five subcategories (melanocytic nevus, actinic keratosis, benign keratosis, dermatofibroma, and vascular) and malignant lesions into two subcategories (melanoma and basal cell carcinoma). The frozen weights of the CNN developed–trained with correlated images benefited the transfer learning using the same type of images for the subclassification of benign and malignant classes. The proposed multistage and multiclass technique improved the classification accuracy of the online ISIC2018 skin lesion dataset by up to 93.4% for benign and malignant class identification. Furthermore, a high accuracy of 96.2% was achieved for subclassification of both classes. Sensitivity, specificity, precision, and F1-score metrics further validated the effectiveness of the proposed multistage and multiclass framework. Compared to existing CNN models described in the literature, the proposed approach took less time to train and had a higher classification rate.
Drug-Free Noninvasive Thermal Nerve Block: Validation of Sham Devices
Authors: Fishman Michael A. , Chitneni Ahish , Abd-Elsayed Alaa , Grodofsky Samuel , Scherer Ashley M. , Schetzner Brendan , Klusek Malvina , Popielarski Stephen R. , Meloni Stephen , Falowski Steven , Kim Philip , Slavin Konstantin V. , Silberstein Stephen D.
Published: December 2023

Headache is a leading cause of disability and suffering. One major challenge in developing device treatments is demonstrating their efficacy given devices’ often-high placebo rate. This paper reviews the importance of validating sham devices as part of finalizing the design for larger-scale prospective randomized controlled trials in patients with chronic headache as well as the results of a prospective, single-blind trial to validate two potential sham noninvasive thermal nerve block devices. Study participants were trained to self-administer thermal nerve block treatment using sham devices in an office visit. Two different sham systems with different temperature profiles were assessed. Devices were offered for patients to use daily at-home for one week to assess the durability of sham placebo effects before participants were given active treatment in a second office visit followed by another optional week of self-administered active treatment at-home use. Sham treatments reduced pain scores by an average of 31% from 6.0 ± 2.3 to 4.3 ± 3.3, including two participants who fell asleep during the in-office treatment and woke up with no pain, but whose pain recurred after returning home during at-home use of the sham system. In-office active treatments reduced pain scores by 52% from 6.7 ± 2.1 to 3.3 ± 2.9 with sustained pain relief during optional at-home use. Successful blinding for the study was confirmed with an ideal Bang’s Blinding Index of 0 and an ideal James’ Blinding Index of 1. Both the sham and active treatments were viewed by participants as highly credible, and credibility increased from the beginning to end of sham treatments on average.
Assisted Cycle Therapy (ACT) Improved Self-Efficacy and Exercise Perception in Middle-Age Adults with Down Syndrome
Authors: Ringenbach Shannon D. R. , Arnold Nathaniel E. , Tucker Kori , Rand Miya K. , Studenka Breanna E. , Ringenbach Stockton B. , Chen Chih-Chia
Published: December 2023

Alzheimer’s disease is prevalent in persons with Down syndrome (DS) as early as their 30s and presents as decreased social interaction, coordination, and physical activity. Therefore, changing attitudes and beliefs about exercise is key to increasing motivation for physical activity especially in middle-age adults with DS. The aim of this study was to examine the effects of Assisted Cycle Therapy (ACT) on self-efficacy and exercise perception in middle-age adults with Down syndrome (DS) following an exercise intervention three times a week for 8 weeks. Twelve participants were in the ACT group in which a motor assisted their cycling to be performed at least 30% faster than voluntary cycling (VC), 10 participants were in the voluntary cycling group, and two participants were in the no cycling (NC) group. The results showed that both exercise groups (i.e., ACT and VC) improved in their self-efficacy after the 8-week intervention. In addition, exercise perception improved following ACT, but not VC or NC. Our results are discussed with respect to their future implications for exercise in the DS population. The results can be attributed to differences in effort required by each intervention group as well as the neurotrophic factors that occur when muscle contractions create synaptic connections resulting in improvement in cognition and feelings of satisfaction.
Body Mass Index: Influence on Interpersonal Style, Basic Psychological Needs, Motivation, and Physical Activity Intention in Physical Education—Differences between Gender and Educational Stage
Authors: Manzano-Sánchez David , Gómez-Mármol Alberto , Gómez-López Manuel
Published: December 2023

The present research study’s main objective was to find out whether there is a relationship between the body mass index (BMI) and the psychological aspects related to motivation, needs such as autonomy, competence, and social relationships, and the intention of being physically active in Physical Education students in Primary and Secondary Education. To achieve this, a total of 574 students (mean = 13.66; standard deviation = 1.96) participated in this study, to whom a series of questionnaires was administered once permission had been obtained from the centers the students attended, alongside the latter’s acceptance to participate in the study. The main results showed that the students with a higher BMI were those who had lower self-determined motivation values with regard to the three basic psychological needs observed and the intention of physical activity. In turn, our differential analysis identified that the students attending Primary Education had lower values of BMI, motivation, and intention to be physically active than the students attending Secondary Education, without finding differences based on the gender of the participants. The need to keep on investigating this topic is consequently gathered, using direct techniques for measuring BMI or proposing mixed research designs.