bluuebunny/crossref_metadata_2025 huggingface dataset:
Estimating Network Parameters From Combined Dynamics of Firing Rate and Irregularity of Single Neurons
Authors: Hamaguchi Kosuke , Riehle Alexa , Brunel Nicolas
Published: August 2010

High firing irregularity is a hallmark of cortical neurons in vivo, and modeling studies suggest a balance of excitation and inhibition is necessary to explain this high irregularity. Such a balance must be generated, at least partly, from local interconnected networks of excitatory and inhibitory neurons, but the details of the local network structure are largely unknown. The dynamics of the neural activity depends on the local network structure; this in turn suggests the possibility of estimating network structure from the dynamics of the firing statistics. Here we report a new method to estimate properties of the local cortical network from the instantaneous firing rate and irregularity (CV2) under the assumption that recorded neurons are a part of a randomly connected sparse network. The firing irregularity, measured in monkey motor cortex, exhibits two features; many neurons show relatively stable firing irregularity in time and across different task conditions; the time-averaged CV2 is widely distributed from quasi-regular to irregular (CV2 = 0.3–1.0). For each recorded neuron, we estimate the three parameters of a local network [balance of local excitation-inhibition, number of recurrent connections per neuron, and excitatory postsynaptic potential (EPSP) size] that best describe the dynamics of the measured firing rates and irregularities. Our analysis shows that optimal parameter sets form a two-dimensional manifold in the three-dimensional parameter space that is confined for most of the neurons to the inhibition-dominated region. High irregularity neurons tend to be more strongly connected to the local network, either in terms of larger EPSP and inhibitory PSP size or larger number of recurrent connections, compared with the low irregularity neurons, for a given excitatory/inhibitory balance. Incorporating either synaptic short-term depression or conductance-based synapses leads many low CV2 neurons to move to the excitation-dominated region as well as to an increase of EPSP size.
Effect of Dietary Conjugated Linoleic Acid Supplementation on Early Inflammatory Responses during Cutaneous Wound Healing
Authors: Park Na-Young , Valacchi Giuseppe , Lim Yunsook
Published: August 2010

Inflammatory response is considered the most important period that regulates the entire healing process. Conjugated linoleic acid (CLA), a class of linoleic acid positional and geometric isomers, is well known for its antioxidant and anti-inflammatory properties. We hypothesized that dietary CLA supplementation accelerates cutaneous wound healing by regulating antioxidant and anti-inflammatory functions. To investigate wound closure rates and inflammatory responses, we used a full-thickness excisional wound model after 2-week treatments with control, 0.5%, or 1% CLA-supplemented diet. Mice fed dietary CLA supplementation had reduced levels of oxidative stress and inflammatory markers. Moreover, the wound closure rate was improved significantly in mice fed a 1% CLA-supplemented diet during early stage of wound healing (inflammatory stage). We conclude that dietary CLA supplementation enhances the early stage of cutaneous wound healing as a result of modulating oxidative stress and inflammatory responses.
Fuzzy Linguistic Optimization on Surface Roughness for CNC Turning
Authors: Lan Tian-Syung
Published: August 2010

Surface roughness is often considered the main purpose in contemporary computer numerical controlled (CNC) machining industry. Most existing optimization researches for CNC finish turning were either accomplished within certain manufacturing circumstances or achieved through numerous equipment operations. Therefore, a general deduction optimization scheme is deemed to be necessary for the industry. In this paper, the cutting depth, feed rate, speed, and tool nose runoff with low, medium, and high level are considered to optimize the surface roughness for finish turning based on L9(34) orthogonal array. Additionally, nine fuzzy control rules using triangle membership function with respective to five linguistic grades for surface roughness are constructed. Considering four input and twenty output intervals, the defuzzification using center of gravity is then completed. Thus, the optimum general fuzzy linguistic parameters can then be received. The confirmation experiment result showed that the surface roughness from the fuzzy linguistic optimization parameters is significantly advanced compared to that from the benchmark. This paper certainly proposes a general optimization scheme using orthogonal array fuzzy linguistic approach to the surface roughness for CNC turning with profound insight.
A Refinement of Quasilinearization Method for Caputo′s Sense Fractional‐Order Differential Equations
Authors: Yakar Coşkun , Yakar Ali
Published: August 2010

The method of the quasilinearization technique in Caputo′s sense fractional‐order differential equation is applied to obtain lower and upper sequences in terms of the solutions of linear Caputo′s sense fractional‐order differential equations. It is also shown that these sequences converge to the unique solution of the nonlinear Caputo′s sense fractional‐order differential equation uniformly and semiquadratically with less restrictive assumptions.
Home Range and Habitat Use of the New Zealand Falcon (Falco novaeseelandiae) within a Plantation Forest: A Satellite Tracking Study
Authors: Thomas Bindi , Minot Edward O. , Holland John D.
Published: August 2010

We tracked two adult and three juvenile New Zealand falcons (Falco novaeseelandiae) in Kaingaroa Forest pine plantation from 2002 to 2008 using Argos satellite technology. The home ranges for both adults and juveniles varied, ranging between 44 and 587 km2. The falcons occasionally utilised areas outside the forest and used stands of all ages within the forest, generally in proportion to their availability. For the most part, the juveniles remained within ca. 8 km of their nests and dispersed at 58, 69, and 68 days after fledging. Falcon movement information was obtained from an average of four location points per tracking day per falcon at a putative accuracy of 350 m. The transmitters, including their solar charge capability, performed well in the forest environment. The use of all stand ages highlights the importance of forestry practises that maintain a mosaic of different aged pine stands.
Effects of Dexmedetomidine-Fentanyl Infusion on Blood Pressure and Heart Rate during Cardiac Surgery in Children
Authors: Klamt Jyrson Guilherme , Vicente Walter Villela de Andrade , Garcia Luis Vicente , Ferreira Cesar Augusto
Published: August 2010

Background. The purpose of this study was to access the effects of dexmedetomidine-fentanyl infusion on blood pressure (BP) and heart rate (HR) before surgical stimulation, on their changes to skin incision, and on isoflurane requirement during cardiac surgery in children.Methods. This study had a prospective, randomized, and open-label design. Thirty-two children aged 1 month to 10 years undergoing surgery for repair congenital heart disease (CHD) with CPB were randomly allocated into two groups: group MDZ received midazolam 0.2 mg·kg−1·h−1and group DEX received dexmedetomidine 1 g·kg−1·h−1during the first hour followed by half of these rates of infusions thereafter. Both group received fentanyl 10 g·kg−1, midazolam 0.2 mg·kg−1and vecuronium 0.2 mg·kg−1for induction. These same doses of fentanyl and vecuronium were infused during the first hour then reduced to half. The infusions started after induction and maintained until the end of surgery. Isoflurane was given briefly to control hyperdynamic response to skin incision and sternotomy.Results. In both groups, systolic blood pressure (sBP) and heart rate (HR) decreased significantly after one hour of infusion of the anesthetic solutions, but there were significantly less increase in diastolic blood pressure, sBP, and HR, and less patients required isoflurane supplementation to skin incision in the patients of the DEX group.Discussion. Dexmedetomidine infusion without a bolus appears to be an effective adjunct to fentanyl anesthesia in control of hemodynamic responses to surgery for repair of CHD in children.
Solution for Ill-Posed Inverse Kinematics of Robot Arm by Network Inversion
Authors: Ogawa Takehiko , Kanada Hajime
Published: August 2010

In the context of controlling a robot arm with multiple joints, the method of estimating the joint angles from the given end-effector coordinates is called inverse kinematics, which is a type of inverse problems. Network inversion has been proposed as a method for solving inverse problems by using a multilayer neural network. In this paper, network inversion is introduced as a method to solve the inverse kinematics problem of a robot arm with multiple joints, where the joint angles are estimated from the given end-effector coordinates. In general, inverse problems are affected by ill-posedness, which implies that the existence, uniqueness, and stability of their solutions are not guaranteed. In this paper, we show the effectiveness of applying network inversion with regularization, by which ill-posedness can be reduced, to the ill-posed inverse kinematics of an actual robot arm with multiple joints.
Effect of Zoledronic Acid on Bone Mineral Density in Men with Prostate Cancer Receiving Gonadotropin-Releasing Hormone Analog
Authors: Kapoor Anoop , Gupta Ankur , Desai Nilay , Ahn Hongshik
Published: August 2010

Background. Loss of bone density with androgen deprivation therapy for prostate cancer is well recognized. We assessed the effects of quarterly infusion of zoledronic acid on bone mineral density (BMD) and markers of bone turnover over a one-year period in men receiving gonadotropin-releasing hormone analog (GnRH-a) for prostate cancer.Methods. 41 subjects were randomly assigned to treatment with zoledronic acid (4 mg) IV infusion or placebo every 3 months. The primary endpoint was the change in the lumbar spine BMD after 12 months of treatment.Results. The change in vertebral BMD in the zoledronic acid group () was significantly () greater than the change in the placebo group () as was the change in left femoral neck BMD ( for the zoledronic acid group versus for the placebo group). The decrease in biochemical markers of bone turnover was significantly () greater in the zoledronic acid group compared to the placebo group.Conclusion. Quarterly infusion of zoledronic acid for 1 year improved vertebral and left femoral neck BMD with a decrease in bone turnover markers in men on GnRH-a treatment. Zoledronic acid treatment appears to be promising in men with low BMD receiving GnRH-a treatment.
Crucial Roles for Protein Kinase C Isoforms in Tumor-Specific Killing by Apoptin
Authors: Jiang Jie , Cole Daryl , Westwood Nigel , Macpherson Lee , Farzaneh Farzin , Mufti Ghulam , Tavassoli Mahvash , Gäken Joop
Published: August 2010

The chicken anemia virus–derived protein apoptin induces apoptosis in a variety of human malignant and transformed cells but not in normal cells. However, the mechanisms through which apoptin achieves its selective killing effects are not well understood. We developed a lentiviral vector encoding a green fluorescent protein–apoptin fusion gene (LV-GFP-AP) that can efficiently deliver apoptin into hematopoietic cells. Apoptin selectively killed the human multiple myeloma cell lines MM1.R and MM1.S, and the leukemia cell lines K562, HL60, U937, KG1, and NB4. In contrast, normal CD34+ cells were not killed and maintained their differentiation potential in multilineage colony formation assays. In addition, dexamethasone-resistant MM1.R cells were found to be more susceptible to apoptin-induced cell death than the parental matched MM1.S cells. Death susceptibility correlated with increased phosphorylation and activation of the apoptin protein in MM1.R cells. Expression array profiling identified differential kinase profiles between MM1.R and MM1.S cells. Among these kinases, protein kinase Cβ (PKCβ) was found by immunoprecipitation and in vitro kinase studies to be a candidate kinase responsible for apoptin phosphorylation. Indeed, shRNA knockdown or drug-mediated inhibition of PKCβ significantly reduced apoptin phosphorylation. Furthermore, apoptin-mediated cell death proceeded through the upregulation of PKCβ, activation of caspase-9/3, cleavage of the PKCδ catalytic domain, and downregulation of the MERTK and AKT kinases. Collectively, these results elucidate a novel pathway for apoptin activation involving PKCβ and PKCδ. Further, they highlight the potential of apoptin and its cellular regulators to purge bone marrow used in autologous transplantation for multiple myeloma. Cancer Res; 70(18); 7242–52. ©2010 AACR.
Successful Treatment of Melanoma Brain Metastases with Adoptive Cell Therapy
Authors: Hong Jenny J. , Rosenberg Steven A. , Dudley Mark E. , Yang James C. , White Donald E. , Butman John A. , Sherry Richard M.
Published: August 2010

Purpose: To determine the objective response rate and response duration of melanoma brain metastases to adoptive cell therapy (ACT) with autologous antitumor lymphocytes plus interleukin-2 following a lymphodepleting preparative regimen.

Methods: Between 2000 and 2009, 264 patients with metastatic melanoma received ACT, consisting of cyclophosphamide and fludarabine with or without total body irradiation, followed by the infusion of autologous tumor-infiltrating lymphocytes (TIL) or autologous peripheral blood lymphocytes retrovirally transduced to express a T-cell receptor (TCR) that recognized the melanocyte differentiation antigens gp-100 or MART-1. From this group, 26 patients were retrospectively identified to have had untreated brain metastases and extracranial disease before receiving ACT. The response rate and duration of melanoma brain metastases, as well as the overall response rate, response duration, and survival for these patients, are presented.

Results: Seventeen of these 26 patients received ACT with TIL. Seven of these patients (41%) achieved a complete response in the brain, and six patients achieved an overall partial response. In the nine patients that received TCR-transduced lymphocytes, two patients achieved a complete response in the brain (22%) and one of these two achieved an overall partial response. One patient developed a tumor-associated subarachnoid hemorrhage during the thrombocytopenic phase of therapy and had an uneventful metastatectomy.

Conclusion: ACT with a nonmyeloablative preparative regimen using either TIL- or TCR gene–transduced cells and interleukin-2 can mediate complete and durable regression of melanoma brain metastases. This strategy can be used safely in selected patients with metastatic melanoma to the brain. Clin Cancer Res; 16(19); 4892–8. ©2010 AACR.
Chk1-Dependent Constitutive Phosphorylation of BLM Helicase at Serine 646 Decreases after DNA Damage
Authors: Kaur Sarabpreet , Modi Priyanka , Srivastava Vivek , Mudgal Richa , Tikoo Shweta , Arora Prateek , Mohanty Debasisa , Sengupta Sagar
Published: August 2010

BLM helicase, the protein mutated in Bloom syndrome, is involved in signal transduction cascades after DNA damage. BLM is phosphorylated on multiple residues by different kinases either after stress induction or during mitosis. Here, we have provided evidence that both Chk1 and Chk2 phosphorylated the NH2-terminal 660 amino acids of BLM. An internal region within the DExH motif of BLM negatively regulated the Chk1/Chk2-dependent NH2-terminal phosphorylation event. Using in silico analysis involving the Chk1 structure and its known substrate specificity, we predicted that Chk1 should preferentially phosphorylate BLM on serine 646 (Ser646). The prediction was validated in vitro by phosphopeptide analysis on BLM mutants and in vivo by usage of a newly generated phosphospecific polyclonal antibody. We showed that the phosphorylation at Ser646 on BLM was constitutive and decreased rapidly after exposure to DNA damage. This resulted in the diminished interaction of BLM with nucleolin and PML isoforms, and consequently decreased BLM accumulation in the nucleolus and PML nuclear bodies. Instead, BLM relocalized to the sites of DNA damage and bound with the damage sensor protein, Nbs1. Mutant analysis confirmed that the binding to nucleolin and PML isoforms required Ser646 phosphorylation. These results indicated that Chk1-mediated phosphorylation on BLM at Ser646 might be a determinant for regulating subnuclear localization and could act as a marker for the activation status of BLM in response to DNA damage. Mol Cancer Res; 8(9); 1234–47. ©2010 AACR.
Body Size and Incident Colorectal Cancer: A Prospective Study of Older Women
Authors: Oxentenko Amy S. , Bardia Aditya , Vierkant Robert A. , Wang Alice H. , Anderson Kristin E. , Campbell Peter T. , Sellers Thomas A. , Folsom Aaron R. , Cerhan James R. , Limburg Paul J.
Published: August 2010

Obesity is a controversial risk factor for colorectal cancer (CRC) in older women. We evaluated associations between multiple body size parameters and incident CRC in the prospective, population-based Iowa Women's Health Study (IWHS). IWHS participants, ages 55 to 69 years, provided data regarding height; weight; weight at ages 50, 40, 30, 18 years; hip circumference; and waist circumference at baseline (1986). Derived variables included body mass index (BMI), waist-to-hip ratio (WHR), and “overweight years” (OWY; conceptually similar to cigarette pack-years). Incident CRC cases (n = 1,464) were ascertained from the State Health Registry of Iowa, through 2005. Multivariable Cox regression models were fit to estimate body size–associated CRC risks. Among 36,941 women (619,961 person-years), baseline height, weight, BMI, hip circumference, waist circumference, and WHR were all positively associated with incident CRC (Ptrend ≤ 0.003 for each). Baseline BMI yielded the highest CRC risk estimates (obese III versus normal, RR = 1.56; 95% CI = 1.10–2.22; Ptrend < 0.001) and was more closely associated with distal than proximal tumors (Ptrend < 0.001 versus 0.06). Conversely, height was more closely associated with proximal than distal tumors (Ptrend < 0.001 versus 0.04). Other body size parameters were less predictive of incident CRC. These data strongly support a positive association between increased body size and CRC risk among older women. Further investigation of when increased body size has the greatest effect on CRC risk (i.e., early adulthood versus later adulthood) might also be informative, particularly with respect to defining subsite-specific pathways of colorectal carcinogenesis. Cancer Prev Res; 3(12); 1608–20. ©2010 AACR.
Chemoimmunotherapy Reduces the Progression of Multiple Myeloma in a Mouse Model
Authors: Sharabi Amir , Laronne-Bar-On Ayelet , Meshorer Asher , Haran-Ghera Nechama
Published: August 2010

Multiple myeloma (MM) is a B-cell malignancy characterized by clonal proliferation of malignant plasma cells in the bone marrow. Recently, we showed a correlation between increased ratios of functional regulatory T cells (Treg) and disease progression in a unique mouse model that mimics the human disease. Cyclophosphamide (CYC) is a cytotoxic alkylating agent widely used in chemotherapeutic regimens. Low-dose CYC was previously reported to selectively reduce Treg levels and to contribute to immunostimulation. Our objectives were (a) to determine whether treatment using a low-dose CYC could reduce MM progression and (b) to further characterize the modes of action underlying these effects. We found that both low- and high-dose CYC given to sick mice with hind limb paralysis resulted in the disappearance of the paralysis, the replacement of plasma tumor cells in the bone marrow by normal cell populations, and a significant prolongation of survival. However, only low-dose CYC treatment decreased the incidence of MM. Low-dose CYC rendered Tregs susceptible to apoptosis because of the downregulation of Bcl-xL and CTLA-4 in these cells, and a decreased production of interleukin 2 by effector CD4 cells. Moreover, using this treatment, we noted the recovery of IFN-γ–producing natural killer T cells and maturation of dendritic cells. Treatment of tumor-bearing mice with repeated administrations of low-dose CYC at longer time intervals (coinciding with the blocked renewal of Tregs) resulted in reduced tumor load, and the prevention or delay of disease recurrence, thereby breaking immune tolerance against MM tumor cells. Cancer Prev Res; 3(10); 1265–76. ©2010 AACR.
CCL5, CXCL10 and CXCL11 Chemokines in Patients with Active and Stable Relapsing-Remitting Multiple Sclerosis
Authors: Szczuciński Adam , Losy Jacek
Published: August 2010

Objective: Chemokines are involved in the migration of inflammatory cells to the central nervous system in multiple sclerosis (MS). The aim of our study was to estimate the concentrations of CCL5, CXCL10 and CXCL11 in serum and cerebrospinal fluid (CSF) samples of relapsing-remitting MS (RRMS) patients during both relapse and stable disease, and to compare the results with those of controls. We also decided to evaluate the effect of methylprednisolone (MP) therapy on CCL5, CXCL10 and CXCL11 serum concentrations in MS patients with relapse. Methods: The study groups consisted of 17 RRMS patients during relapse, 30 RRMS patients in remission and 25 patients with tension headache with no symptoms of inflammatory disease as controls. In the group of relapsing MS patients, blood samples were obtained before steroid therapy and after a 5-day treatment with MP at a dose of 1 g i.v. once daily. Chemokine levels were measured by ELISA. Results: CXCL10 levels were significantly higher in the CSF of MS patients both during relapse (mean ± SD, 298.2 ± 143.8 pg/ml) and stable disease (323.7 ± 183 pg/ml) in comparison with the control group (152.4 ± 97.7 pg/ml; p < 0.001). CSF levels of CCL5 were significantly higher in relapsing MS patients (8.74 ± 6.18 pg/ml) in comparison with stable MS patients (4.4 ± 3.9 pg/ml, p = 0.005). CXCL11 levels of MS patients did not significantly differ from control values. There was no effect of MP therapy on serum levels of CCL5, CXCL10 and CXCL11. Conclusions: These observations suggest involvement of CXCL10 and CCL5 but not CXCL11 in the pathogenesis of MS. CCL5 may induce the recruitment of inflammatory cells in acute-stage MS.
Autoimmune Bullous Skin Diseases Occurring under Anti-Tumor Necrosis Factor Therapy: Two Case Reports
Authors: Boussemart L. , Jacobelli S. , Batteux F. , Goulvestre C. , Grange P. , Carlotti A. , Morini J.P. , Gorin I. , Ziza J.M. , Avril M.F. , Dupin N.
Published: August 2010

Background: Anti-tumor necrosis factor (TNF) agents are increasingly being used for a rapidly expanding number of rheumatic and systemic diseases. As a result of this use, and of the longer follow-up periods of treatment, there are a growing number of reports of the development of autoimmune processes related to anti-TNF agents. The use of anti-TNF agents has been associated with more and more cases of autoimmune diseases, principally cutaneous vasculitis, lupus-like syndrome, systemic lupus erythematosus and interstitial lung disease. Observations: We report 2 cases of autoimmune bullous skin disease occurring in patients undergoing TNF-targeted therapy: a bullous pemphigoid and a pemphigus foliaceus. Both patients were treated by anti-TNF agents for rheumatoid arthritis and showed improvement following interruption of that treatment. Here, we discuss the relationship between anti-TNF therapy and the occurrence of autoimmune bullous disease. Conclusion: Anti-TNF agents should be considered as a potential cause of drug-induced autoimmune bullous skin disease.
Snacking Habits and Caries in Young Children
Authors: Johansson I. , Lif Holgerson P. , Kressin N.R. , Nunn M.E. , Tanner A.C.
Published: August 2010

Dental caries is caused by a combination of infection and diet. This disease, if left untreated, may lead to pain, and impair the quality of life, nutritional status and development of young children. The objective was to investigate the association between snacking and caries in a population at high risk of dental caries. American preschool children (n = 1,206) were recruited in the offices of paediatricians. Data on sociodemographic characteristics, oral hygiene, breast-feeding, use of bottle and snacking were collected by questionnaire. Plaque presence, the number of teeth and their caries status (deft) were scored. The children sampled were 61% Black, 27% White and 10% Asian. Of the 1- to 2-, 2- to 3- and 3- to 4-year-old children, 93.8, 82.4 and 77.3% were caries free, and their mean caries scores were 0.16, 0.58 and 0.93, respectively. Multivariate partial least squares (PLS) modelling revealed plaque presence, lowest income, descriptors for tooth exposure time (number of teeth and age) and cariogenic challenge (total intake of sugar-containing snacks and chips/crisps, and chips intake with a sugar-containing drink) to be associated with more caries. These differences were also found in univariate analyses; in addition, children who continued breast-feeding after falling asleep had significantly higher deft values than those who did not. PLS modelling revealed that eating chips clustered with eating many sweet snacks, candies, popcorn and ice cream. We conclude that, in addition to the traditional risk indicators for caries – presence of plaque, sugar intake and socioeconomic status –, consumption of chips was associated with caries in young children.
Contribution of Angiogenic Factors in a Rat Model of Pre-Eclampsia
Authors: Agunanne E.E. , Uddin M.N. , Horvat D. , Puschett J.B.
Published: August 2010

Background/Aims: Pre-eclampsia is a disorder that results in significant feto-maternal complications with yet no definitive pharmacologic intervention. One postulated etiologic mechanism is an imbalance between circulating pro-angiogenic and anti-angiogenic factors. We investigated these factors sequentially throughout pregnancy (19–21 days) in our rat model of pre-eclampsia, which involves the imposition of excessive volume expansion. Methods: We evaluated the status of the pro-angiogenic and anti-angiogenic factors at the following time points: 3–5, 7–10 and 17–20 days of gestation. Results: We have previously determined that the urinary excretion of the circulating bufodienolide, marinobufagenin, is elevated at the 3- to 5-day time period, prior to the advent of hypertension and proteinuria. At 3–5 days of pregnancy, there was no evidence of angiogenic imbalance in the normal pregnant (NP) and ‘pre-eclamptic’ (PDS) rats. At the 7- to 10-day time point, plasma PlGF was greater in the NP rats than in the PDS group (p < 0.05). The plasma sFlt-1/PlGF ratio in the PDS animals was greater than that in the NP rats (p < 0.05). The placental sFlt-1 and sFlt-1/PlGF ratio were greater in the PDS rats than in NP rats (p < 0.05). These changes were also present at the 17- to 20-day time point in both plasma and placenta. The administration of resibufogenin, an antagonist of marinobufagenin, early in pregnancy, prevented angiogenic imbalance. Conclusion: We conclude that angiogenic imbalance plays a role in the pathogenesis of pre-eclampsia in this rat model. Furthermore, the earliest event in the pathogenetic sequence appears to be the secretion and elaboration of marinobufagenin.
Striking Increases in Carotid Artery Wall Thickness in Healthy Subjects
Authors: Meijer Rudy , Störk Stefan , Evans Gregory W. , Grobbee Diederick E. , Bots Michiel L.
Published: August 2010

Background: Atherosclerosis has long been thought to develop over time in a linear manner from gradual wall thickening to advanced thick lesions. However, evidence has emerged indicating a phasic rather than linear progression with time. A major reason for this non-linear pattern appears to be the occurrence of hemorrhages in the arterial wall, although data on this issue are still scarce. We studied the occurrence of temporarily impressive thickenings of the carotid arterial wall in a cohort of healthy postmenopausal women who were followed up for 3 years with regular carotid ultrasound examinations. Methods: The women were the European participants of a randomized placebo-controlled trial into the effect of hormone replacement therapy on progression of carotid intima-media thickness (CIMT). For a period of 3 years, the women underwent a standardized carotid ultrasound protocol every 6 months. Common, bifurcation and internal carotid segments were scanned on both sides, stored on videotape, and the near and far wall CIMT was measured on defined angles and segments, also in areas of plaque. Adverse events were routinely recorded. At the completion of the study, all segment-specific measurements were evaluated for outliers. Images were retrieved from videotape and evaluated whether the outlier resulted from a real morphological change or ‘measurement error’. Results: The 509 healthy postmenopausal women, free from previous symptomatic cardiovascular disease, underwent 3,812 carotid ultrasound scans during the study, and 44,924 carotid segments were evaluated. In 203 segments of 188 participants outliers were observed. True morphological changes were found in 12 participants, equivalent to a 3-year risk of 2.4%. These changes did not give rise to clinical symptoms. In the 6 women of whom we had follow-up measurements, the changes were reversed within 6–12 months. Conclusion: We observed acute increases in CIMT among 2.4% of healthy postmenopausal European women followed for 3 years. When assuming these were the result of vessel wall hemorrhages, our findings add to the body of evidence suggesting that vessel wall hemorrhages contribute to atherosclerosis development and also appear to occur clinically silent.
An Intergenerational Approach in the Promotion of Balance and Strength for Fall Prevention – A Mini-Review
Authors: Granacher Urs , Muehlbauer Thomas , Gollhofer Albert , Kressig Reto W. , Zahner Lukas
Published: August 2010

The risk of sustaining a fall is particularly high in children and seniors. Deficits in postural control and muscle strength either due to maturation, secular declines or biologic aging are two important intrinsic risk factors for falls. During life span, performance in variables of static postural control follows a U-shaped curve with children and seniors showing larger postural sway than healthy adults. Measures of dynamic postural control (i.e. gait speed) as well as isometric (i.e. maximal strength) and dynamic muscle strength (i.e. muscular power) follow an inverted U-shaped curve during life span, again with children and seniors showing deficits compared to adults. There is evidence that particularly balance and resistance training are effective in counteracting these neuromuscular constraints in both children and seniors. Further, these training regimens are able to reduce the rate of sustaining injuries and falls in these age groups. An intergenerational intervention approach is suggested to enhance the effectiveness of these training programs by improving compliance and increasing motivation of children and seniors exercising together. Thus, the objectives of this mini-review are: (1) to describe the epidemiology and etiology of falls in children and seniors; (2) to discuss training programs that counteract intrinsic fall risk factors by reducing the rate of falling, and (3) to present an intergenerational approach that has the potential to make training programs even more effective by including children and seniors together in one exercise group.
Chocolate Intake and Incidence of Heart Failure
Authors: Mostofsky Elizabeth , Levitan Emily B. , Wolk Alicja , Mittleman Murray A.
Published: August 2010

Background—

Randomized clinical trials have shown that chocolate intake reduces systolic and diastolic blood pressure, and observational studies have found an inverse association between chocolate intake and cardiovascular disease. The aim of this study was to investigate the association between chocolate intake and incidence of heart failure (HF).

Methods and Results—

We conducted a prospective cohort study of 31 823 women aged 48 to 83 years without baseline diabetes or a history of HF or myocardial infarction who were participants in the Swedish Mammography Cohort. In addition to answering health and lifestyle questions, participants completed a food-frequency questionnaire. Women were followed from January 1, 1998, through December 31, 2006, for HF hospitalization or death through the Swedish inpatient and cause-of-death registers. Over 9 years of follow-up, 419 women were hospitalized for incident HF (n=379) or died of HF (n=40). Compared with no regular chocolate intake, the multivariable-adjusted rate ratio of HF was 0.74 (95% CI, 0.58 to 0.95) for women consuming 1 to 3 servings of chocolate per month, 0.68 (95% CI, 0.50 to 0.93) for those consuming 1 to 2 servings per week, 1.09 (95% CI, 0.74 to 1.62) for those consuming 3 to 6 servings per week, and 1.23 (95% CI, 0.73 to 2.08) for those consuming ≥1 servings per day (
P
=0.0005 for quadratic trend).

Conclusions—

In this population, moderate habitual chocolate intake was associated with a lower rate of HF hospitalization or death, but the protective association was not observed with intake of ≥1 servings per day.
Int6
/eIF3e Silencing Promotes Functional Blood Vessel Outgrowth and Enhances Wound Healing by Upregulating Hypoxia-Induced Factor 2α Expression
Authors: Chen Li , Endler Alexander , Uchida Kazuyo , Horiguchi Shin-ichiro , Morizane Yoshihito , Iijima Osamu , Toi Masakazu , Shibasaki Futoshi
Published: August 2010

Background—

We previously identified INT6/eIF3e as a novel regulator of hypoxia-inducible factor 2α (HIF2α) activity. Small interfering RNA (siRNA)–Int6 adequately stabilized HIF2α, even under normoxic conditions, and thereby enhanced the expression of several angiogenic factors in vitro, suggesting that siRNA-Int6 may induce angiogenesis in vivo.

Methods and Results—

We demonstrated a 6- to 8-fold enhanced formation of normal arteries and veins in the subcutaneous regions of adult mice 5 days after a single siRNA-Int6 application. Subcutaneous fibroblasts were identified as the major source of secreted angiogenic factors that led to the formation of functional vessels during
Int
6
silencing. Fibroblasts transfected ex vivo with siRNA-Int6 induced potent neoangiogenesis when transplanted into a subcutaneous region of nude mice. Application of siRNA-Int6 promoted neoangiogenesis in the area surrounding the injury in wound healing models, including genetically diabetic mice, thereby accelerating the closure of the injury. HIF2α accumulation caused by siRNA-Int6 was confirmed as the unequivocal cause of the angiogenesis by an in vivo angiogenesis assay. Further analysis of the
Int
6 silencing–induced neoangiogenesis revealed that a negative feedback regulation of HIF2α stability was caused by HIF2α-induced transcription of
Int6
via hypoxia-response elements in its promoter. Thus, siRNA-Int6 temporarily facilitates an accumulation of HIF2α protein, leading to hypoxia-independent transcription of angiogenic factors and concomitant neoangiogenesis.

Conclusions—

We suggest that the pathway involving INT6/HIF2α acts as a hypoxia-independent master switch of functional angiogenesis; therefore, siRNA-Int6 application might be of clinical value in treating ischemic diseases such as heart and brain ischemia, skin injury, and diseases involving obstructed vessels.
Positive Relationship Between Plasma Leptin Level and Hypertension
Authors: Shankar Anoop , Xiao Jie
Published: August 2010

Leptin is an adipose tissue-derived hormone shown to be related to metabolic, inflammatory, and hemostatic factors involved in hypertension development. Animal studies suggest that higher leptin levels may activate the sympathetic nervous system and cause elevations in blood pressure (BP). However, few studies have examined the association between leptin and hypertension in humans. Also it is not clear whether this association is present among women as well as men. Therefore, we examined the association between plasma leptin levels and hypertension in a representative sample of US adults. We examined the third National Health and Nutrition Examination Survey participants >20 years of age (n=5599; 54.7% women). Plasma leptin levels were categorized into quartiles (women: <7.68, 7.68 to 13.18, 13.19 to 21.70, >21.70 fg/L; men: <2.64, 2.64 to 4.36, 4.37 to 7.12, >7.12 fg/L). Hypertension was defined as BP-reducing medication use or having systolic BP ≥140 mm Hg and/or diastolic BP ≥90 mm Hg. We found that higher plasma leptin levels were positively associated with hypertension after adjusting for age, sex, race/ethnicity, education, smoking, alcohol intake, body mass index, diabetes mellitus, serum cholesterol, and C-reactive protein. Compared with quartile 1 of leptin (referent), the odds ratio (95% CI) of hypertension associated with quartile 4 was 1.89 (1.24 to 2.09;
P
for trend=0.0036). Subgroup analyses examining the relation between leptin and hypertension by sex and body mass index categories also showed a consistent positive association. In conclusion, higher plasma leptin levels are associated with hypertension both among women as well as men in a representative sample of US adults.
Impaired 3′,5′-Cyclic Adenosine Monophosphate–Mediated Signaling in Immediate Early Responsive Gene X-1–Deficient Vascular Smooth Muscle Cells
Authors: Shahid Mohd , Shen Li , Seldin David C. , Lu Bao , Ustyugova Irina V. , Chen Xinyuan , Zapol Warren M. , Wu Mei X.
Published: August 2010

Gene-targeted deletion of the immediate early responsive gene X-1 (IEX-1) results in a significant increase in systemic arterial blood pressure, but the underlying mechanism is not understood. Studies of arterial reactivity in isolated aortas revealed normal endothelium-dependent and -independent vasorelaxation and vasoconstriction but reduced cAMP-dependent vasorelaxation in the absence of IEX-1. This defect in cAMP signaling was also evident in endothelium-denuded aortic rings, consistent with the enhancement of mitochondrial O
2
·−
production only in IEX-1-deficient vascular smooth muscle cells, not in endothelial cells. Excessive production of reactive oxygen species at mitochondria augmented the expression of Gα
i2
, suppressing cAMP production in vascular smooth muscle cells. The role of mitochondrial reactive oxygen species in the upregulation of Gα
i2
leading to the development of hypertension was supported by the ability of antioxidant or pertussis toxin to restore the cAMP-dependent vasorelaxation to a normal level and reverse established hypertension in IEX-1 homozygous knockout mice. Our results suggest that hypertension in IEX-1 knockout mice may arise primarily from impaired cAMP signaling induced by overproduction of mitochondrial reactive oxygen species in vascular smooth muscle cells and demonstrate a causal relationship between mitochondrial dysfunction and cAMP-dependent vasorelaxation.
The ‘work group’: Redressing the balance in Bion’s Experiences in Groups
Authors: French Robert B , Simpson Peter
Published: August 2010

The intention of this article is to explore and develop Wilfred Bion’s theory of groups, and to contribute to its wider application across the social sciences. Bion suggested that groups operate simultaneously in two strictly contrasting ways, based on distinctive mental states, which he called ‘basic-assumption mentality’ and ‘work-group mentality’. He believed that these mentalities determine a group’s capacity to achieve its purposes. However, the development of these ideas has tended to focus on the regressive tendency in group functioning — on basic-assumption mentality. This article attempts to redress the balance by ascribing equal importance to the notion of work-group mentality. First, it extends Bion’s framework, developing the concept of the ‘work group’ in parallel with the ‘basic assumptions’; second, it considers the dynamic relationship between these two mentalities, in order, finally, to explore the application of Bion’s ideas via a case example.
Combined Experimental and Simulation Study of the Cure Kinetics of DCPD
Authors: Aldridge Michael , Shankar Chandreshekar , Changgua Zhen  , Lang Sui  , Kieffer John , Caruso Mary , Moore Jeff
Published: August 2010

In this study, the cure kinetics of dicyclopentadiene was investigated using a combination of inelastic light scattering measurements and molecular-scale simulations. Concurrent Brillouin and Raman scattering served to monitor the structural evolution of the curing network as a function of time, both in terms of network connectivity and the concentration of chemical species present. Density functional theory calculations were used to interpret the measured Raman spectra. Comparison of the measured elastic moduli as a function of the degree of cure with those of structures generated using reactive molecular dynamics simulations provides insight into the reaction mechanism. An unexpected dependence of the reaction rate on the catalyst concentration was found.
Introduction to Special Section for Journal of Cross-Cultural Psychology: Value of Children: A Concept for Better Understanding Cross-Cultural Variations in Fertility Behavior and Intergenerational Relationships
Authors: Trommsdorff Gisela , Nauck Bernhard
Published: August 2010

The concept of value of children (VOC) was introduced three decades ago and has recently gained increasing importance due to ongoing significant demographic changes in many parts of the world, namely declining birth rates and increasing life expectancy. Even though the concept of VOC suggests interdisciplinary and cross-cultural research, these methodologies were only recently used as part of a collaborative international enterprise aiming to revise and extend the original VOC study. The extended study is based on psychological and sociological approaches and makes use of more sophisticated methods of cross-cultural comparison and multilevel analyses. The thematic scope has also been expanded beyond childbearing to childrearing and also deals with parent-child relations over the entire lifespan. The present special section provides an overview of ongoing theoretical and methodological progress on the basis of four selected studies on VOC and intergenerational relations in different cultures.
Routine Online Activity and Internet Fraud Targeting: Extending the Generality of Routine Activity Theory
Authors: Pratt Travis C. , Holtfreter Kristy , Reisig Michael D.
Published: August 2010

Routine activity theory predicts that changes in legitimate opportunity structures (e.g., technology) can increase the convergence of motivated offenders and suitable targets in the absence of capable guardianship. The Internet has fundamentally changed consumer practices and has simultaneously expanded opportunities for cyber-fraudsters to target online consumers. The authors draw on routine activity theory and consumer behavior research to understand how personal characteristics and online routines increase people’s exposure to motivated offenders. Using a representative sample of 922 adults from a statewide survey in Florida, the results of the regression models are consistent with prior research in that sociodemographic characteristics shape routine online activity (e.g., spending time online and making online purchases). Furthermore, indicators of routine online activity fully mediate the effect of sociodemographic characteristics on the likelihood of being targeted for fraud online. These findings support the routine activity perspective and provide a theoretically informed direction for situational crime prevention in a largely unexplored consumer context.
Post-Industrialisation, Job Opportunities and Ethnocentrism
Authors: van der Waal Jeroen , Burgers Jack
Published: August 2010

This article assesses the consequences of the transition to a post-industrial urban economy for labour demand and ethnocentrism in the 22 Dutch urban agglomerations. Using municipal-level data as well as surveys, it is shown that the labour market of the least post-industrial cities yields low labour demand for lower-educated urbanites (upgrading/professionalisation thesis), while the labour market of the most post-industrial cities yields high labour demand for lower-educated urbanites (polarisation thesis). It is furthermore found that lower-educated natives in the former are more ethnocentric than in the latter. However, contrary to what is often claimed in urban studies and the social sciences at large, this proves not to be driven by job scarcity in the least post-industrial cities. The article concludes with suggestions on what might be responsible for this finding.
Determinants of Neighbourhood Satisfaction and Perception of Neighbourhood Reputation
Authors: Permentier Matthieu , Bolt Gideon , van Ham Maarten
Published: August 2010

It has been suggested that the residential mobility behaviour and general well-being of residents of urban neighbourhoods are not only influenced by how residents themselves assess their neighbourhood, but also by how they think other city residents see their neighbourhood: the perceived reputation of the neighbourhood. There is a large body of literature on residents’ satisfaction with their neighbourhood, but much less is known about how residents perceive the reputation of their own neighbourhood. Such knowledge might give important clues on how to improve the well-being of residents in deprived neighbourhoods, not only by directly improving the factors that affect their own level of satisfaction, but also by improving the factors that residents think have a negative effect on the reputation of their neighbourhood. This paper examines whether there are differences in the determinants of neighbourhood satisfaction and the perceived reputation of the neighbourhood. Using data from a purpose-designed survey to study neighbourhood reputations in the city of Utrecht, the Netherlands, it is found that subjective assessment of the dwelling and neighbourhood attributes are more important in explaining neighbourhood satisfaction than in explaining perception of reputation. Objective neighbourhood variables are more important in explaining perception of reputation than in explaining neighbourhood satisfaction.
Bridging and Linking in a Divided Society
Authors: Muir Jenny
Published: August 2010

This paper reports the results of research into social capital levels in the Central Housing Community Network, part of the community consultation structure of the Northern Ireland Housing Executive. Membership of the forum increased the bonding, bridging and linking social capital of its members and appeared to improve community relations, although that was not its stated purpose. However, the empirical link between social capital and the quality of community relations remains unproven. The research provides an example of the state creating a positive space for interaction with civil society through consultation on service delivery issues. In an international policy environment where ‘mixed’ communities are the ideal, the potential of service-based forums to contribute to community cohesion may have been underestimated.
Spatial Mobility Intentions, the Labour Market and Incapacity Benefit Claimants
Authors: Shuttleworth Ian , Green Anne
Published: August 2010

The paper analyses the work-related spatial mobility intentions of incapacity benefit (IB) claimants in Northern Ireland using a new survey dataset. Greater understanding of the prospective mobility of benefit claimants contributes to debates about employability, inclusion in the labour market and arguments about spatial mismatch. The analysis finds that IB claimants are not markedly less mobile than other population groups; that mobility is shaped by age, level of qualification, illness type, ability to drive and motivation; and that geography is important in several ways. The paper concludes by arguing that limited spatial mobility is a good indicator of disadvantage and that spatial mobility should be placed nearer the centre of the design and delivery of labour market policy.