bluuebunny/crossref_metadata_2025 huggingface dataset:
Maternal COVID-19 vaccination status and association with neonatal congenital anomalies
Authors: Santos Janelle , Miller Megan , Branda Megan E. , Mehta Ramila A. , Theiler Regan N.
Published: April 2024

Introduction

Despite recommendations for COVID-19 vaccination in pregnant people, the effect of vaccination on neonatal outcomes remains unknown. We sought to determine the association between COVID-19 vaccination status in pregnancy and presence of neonatally diagnosed congenital anomalies.

Methods

A comprehensive vaccine registry was combined with a delivery database to create a cohort including all patients aged 16–55 years with a delivery event between December 10, 2020 and December 31, 2021 at a hospital within the Mayo Clinic Health System. Pregnancy and neonatal outcomes were analyzed in relation to vaccination status and timing, including a composite measure of congenital anomalies diagnosed in neonatal life. Comparisons between cohorts were conducted using chi-square test for categorical and Kruskal–Wallis test for continuous variables. A multivariable logistic regression was modeled to assess the association with congenital anomalies.

Results

5,096 mother-infant pairs were analyzed. A total of 1,158 were vaccinated, with 314 vaccinated in the first trimester. COVID-19 vaccination status, including vaccination during the first trimester of pregnancy, was not associated with an increased risk of composite congenital anomalies. When further examining congenital anomalies by organ system, we did demonstrate a significant difference in eye, ear, face, neck anomalies between vaccinated and not vaccinated groups (Table 3, Not vaccinated = 2.3%, Vaccinated = 3.3%, p-value 0.04) however we did not demonstrate this difference between the 1st trimester and not vaccinated groups (Not vaccinated = 2.3%, 1st Trimester = 2.5%, p-value 0.77). No differences were found between not vaccinated, vaccinated, or 1st trimester vaccinated groups for any other organ systems. There were no differences in birthweight by gestational age, APGAR scores, incidence of NICU admission, or living status of the neonate by vaccination status.

Conclusion

We add additional information regarding the safety of COVID-19 vaccination status and timing as it pertains to neonatal composite congenital anomalies, with no association demonstrated. Our findings agree with prior literature that COVID-19 vaccination is not associated with adverse pregnancy outcomes or small for gestational age neonates. Further research is needed to elucidate the association between COVID-19 vaccination and eye, ear, face, neck, anomalies.
“Every breath you take”: evaluating sound levels and acoustic characteristics of various neonatal respiratory support and ventilation modalities
Authors: Stummer Sophie , Reuter Christoph , Czedik-Eysenberg Isabella , Bertsch Matthias , Klebermass-Schrehof Katrin , Mader Johannes , Buchmayer Julia , Bartha-Doering Lisa , Berger Angelika , Giordano Vito
Published: April 2024

Background

Early sensory experiences have a significant impact on the later life of preterm infants. The NICU soundscape is profoundly influenced by various modalities of respiratory support or ventilation, which are often mandatory early in the care. The incubator, believed to shield from external noise, is less effective against noise originating inside. The objective of this study was to evaluate the sound levels and characteristics of frequently used respiratory support and ventilation modalities, taking into consideration the developing auditory system of premature infants.

Methods

To evaluate sound dynamics inside and outside an incubator during respiratory support/ventilation, experimental recordings were conducted at the Center for Pediatric Simulation Training of the Medical University Vienna. The ventilator used was a FABIAN HFOI®.

Results

Jet CPAP (Continuous positive airway pressure), whether administered via mask or prongs, generates significantly higher sound levels compared to High-flow nasal cannula (HFNC) and to High-frequency oscillatory ventilation (HFOV) delivered through an endotracheal tube. Upon evaluating the sound spectrum of jet CPAP support, a spectral peak is observed within the frequency range of 4 to 8 kHz. Notably, this frequency band aligns with the range where the hearing threshold of preterm infants is at its most sensitive.

Conclusion

Non-invasive HFNC and invasive HFOV generate lower sound levels compared to those produced by jet CPAP systems delivered via masks or prongs. Moreover, HFNC and HFOV show a reduced acoustic presence within the frequency range where the preterm infant’s hearing is highly sensitive. Therefore, it is reasonable to speculate that the potential for auditory impairment might be more pronounced in preterm infants who require prolonged use of jet CPAP therapy during their time in the incubator.
The inviscid incompressible limit of Kelvin–Helmholtz instability for plasmas
Authors: Briard A. , Ripoll J.-F. , Michael A. , Gréa B.-J. , Peyrichon G. , Cosmides M. , El-Rabii H. , Faganello M. , Merkin V. G. , Sorathia K. A. , Ukhorskiy A. Y. , Lyon J. G. , Retino A. , Bouffetier V. , Ceurvorst L. , Sio H. , Hurricane O. A. , Smalyuk V. A. , Casner A.
Published: April 2024

Introduction: The Kelvin–Helmholtz Instability (KHI) is an interface instability that develops between two fluids or plasmas flowing with a common shear layer. KHI occurs in astrophysical jets, solar atmosphere, solar flows, cometary tails, planetary magnetospheres. Two applications of interest, encompassing both space and fusion applications, drive this study: KHI formation at the outer flanks of the Earth’s magnetosphere and KHI growth from non-uniform laser heating in magnetized direct-drive implosion experiments. Here, we study 2D KHI with or without a magnetic field parallel to the flow. We use both the GAMERA code, which solves the compressible Euler equations, and the STRATOSPEC code, which solves the Navier-Stokes equations under the Boussinesq approximation, coupled with the magnetic field dynamics. GAMERA is a global three-dimensional MHD code with high-order reconstruction in arbitrary nonorthogonal curvilinear coordinates, which is developed for a large range of astrophysical applications. STRATOSPEC is a three-dimensional pseudo-spectral code with an accuracy of infinite order (no numerical diffusion). Magnetized KHI is a canonical case for benchmarking hydrocode simulations with extended MHD options.

Methods: An objective is to assess whether or not, and under which conditions, the incompressibility hypothesis allows to describe a dynamic compressible system. For comparing both codes, we reach the inviscid incompressible regime, by decreasing the Mach number in GAMERA, and viscosity and diffusion in STRATOSPEC. Here, we specifically investigate both single-mode and multi-mode initial perturbations, either with or without magnetic field parallel to the flow. The method relies on comparisons of the density fields, 1D profiles of physical quantities averaged along the flow direction, and scale-by-scale spectral densities. We also address the triggering, formation and damping of filamentary structures under varying Mach number or Atwood number, with or without a parallel magnetic field.

Results: Comparisons show very satisfactory results between the two codes. The vortices dynamics is well reproduced, along with the breaking or damping of small-scale structures. We end with the extraction of growth rates of magnetized KHI from the compressible regime to the incompressible limit in the linear regime assessing the effects of compressibility under increasing magnetic field.

Discussion: The observed differences between the two codes are explained either from diffusion or non-Boussinesq effects.
Ancient hybridization and repetitive element proliferation in the evolutionary history of the monocot genus Amomum (Zingiberaceae)
Authors: Hlavatá Kristýna , Záveská Eliška , Leong-Škorničková Jana , Pouch Milan , Poulsen Axel Dalberg , Šída Otakar , Khadka Bijay , Mandáková Terezie , Fér Tomáš
Published: April 2024

Genome size variation is a crucial aspect of plant evolution, influenced by a complex interplay of factors. Repetitive elements, which are fundamental components of genomic architecture, often play a role in genome expansion by selectively amplifying specific repeat motifs. This study focuses on Amomum, a genus in the ginger family (Zingiberaceae), known for its 4.4-fold variation in genome size. Using a robust methodology involving PhyloNet reconstruction, RepeatExplorer clustering, and repeat similarity-based phylogenetic network construction, we investigated the repeatome composition, analyzed repeat dynamics, and identified potential hybridization events within the genus. Our analysis confirmed the presence of four major infrageneric clades (A–D) within Amomum, with clades A–C exclusively comprising diploid species (2n = 48) and clade D encompassing both diploid and tetraploid species (2n = 48 and 96). We observed an increase in the repeat content within the genus, ranging from 84% to 89%, compared to outgroup species with 75% of the repeatome. The SIRE lineage of the Ty1-Copia repeat superfamily was prevalent in most analyzed ingroup genomes. We identified significant difference in repeatome structure between the basal Amomum clades (A, B, C) and the most diverged clade D. Our investigation revealed evidence of ancient hybridization events within Amomum, coinciding with a substantial proliferation of multiple repeat groups. This finding supports the hypothesis that ancient hybridization is a driving force in the genomic evolution of Amomum. Furthermore, we contextualize our findings within the broader context of genome size variations and repeatome dynamics observed across major monocot lineages. This study enhances our understanding of evolutionary processes within monocots by highlighting the crucial roles of repetitive elements in shaping genome size and suggesting the mechanisms that drive these changes.
Haploid identification in maize
Authors: Dermail Abil , Mitchell Mariah , Foster Tyler , Fakude Mercy , Chen Yu-Ru , Suriharn Khundej , Frei Ursula Karolina , Lübberstedt Thomas
Published: April 2024

Doubled haploid (DH) line production throughin vivomaternal haploid induction is widely adopted in maize breeding programs. The established protocol for DH production includes four steps namelyin vivomaternal haploid induction, haploid identification, genome doubling of haploid, and self-fertilization of doubled haploids. Since modern haploid inducers still produce relatively small portion of haploids among undesirable hybrid kernels, haploid identification is typically laborious, costly, and time-consuming, making this step the second foremost in the DH technique. This manuscript reviews numerous methods for haploid identification from different approaches including the innate differences in haploids and diploids, biomarkers integrated in haploid inducers, and automated seed sorting. The phenotypic differentiation, genetic basis, advantages, and limitations of each biomarker system are highlighted. Several approaches of automated seed sorting from different research groups are also discussed regarding the platform or instrument used, sorting time, accuracy, advantages, limitations, and challenges before they go through commercialization. The past haploid selection was focusing on finding the distinguishable marker systems with the key to effectiveness. The current haploid selection is adopting multiple reliable biomarker systems with the key to efficiency while seeking the possibility for automation. Fully automated high-throughput haploid sorting would be promising in near future with the key to robustness with retaining the feasible level of accuracy. The system that can meet between three major constraints (time, workforce, and budget) and the sorting scale would be the best option.
Identification of Faba bean genetic loci associated with quantitative resistance to the fungus Botrytis fabae, causal agent of chocolate spot
Authors: Webb Anne , Reynolds Tom R. , Wright Tally I. C. , Caiazzo Rosa , Lloyd David C. , Thomas Jane E. , Wood Thomas A.
Published: April 2024

Introduction

Chocolate spot, caused by the ascomycete fungus Botrytis fabae, is a devastating foliar disease and a major constraint on the quality and yield of faba beans (Vicia faba). The use of fungicides is the primary strategy for controlling the disease. However, high levels of partial genetic resistance have been identified and can be exploited to mitigate the disease.

Methods

The partially resistant V. faba cultivar Maris Bead and susceptible Egyptian accession ig70726 were crossed, and a genetic mapping population of 184 individuals was genotyped in the F2 generation and screened for resistance to B. fabae infection in the F3, F5, and F6 generations in a series of field experiments. A high-density linkage map of V. faba containing 3897 DArT markers spanning 1713.7 cM was constructed.

Results

Multiple candidate quantitative trait loci (QTLs) in 11 separate regions of the V. faba genome were identified; some on chromosomes 2, 3, and 6 overlapped with loci previously linked to resistance to Ascochyta leaf and pod blight caused by the necrotrophic fungus Ascochyta fabae. A transcriptomics experiment was conducted at 18 h post-inoculation in seedlings of both parents of the mapping population, identifying several differentially expressed transcripts potentially involved in early stage defence against B. fabae, including cell-wall associated protein kinases, NLR genes, and genes involved in metabolism and response to reactive oxygen species.

Discussion

This study identified several novel candidate QTLs in the V. faba genome that contribute to partial resistance to chocolate spot, but differences between growing seasons highlighted the importance of multi-year phenotyping experiments when searching for candidate QTLs for partial resistance.
Audience reconstructed: social media interaction by BTS fans during live stream concerts
Authors: Upham Finn , Lee Jin Ha , Park So Yeon
Published: April 2024

COVID-19-motivated social distancing made online concerts common practice in 2020 and 2021, with millions logging into streaming sites to see their favorite artists perform in realtime. For some fans, watching alone at home may have been enough, but concert-concurrent surges of social media activity suggest many virtual performance attendees are doing more. To understand why fans would turn their attention from these precious performance streams to social media, we explored Twitter engagement during four live streamed concerts performed by the Kpop group BTS in 2021. In public Tweets sampled by either concert hashtag or a predefined stream of users and keywords, we evaluated patterns in posting rates in relation to concert program events and investigated the content patterns in 1,200 Tweets sampled from four ranges of popularity (number of Retweets during the concert). Across concerts, short “Shout” Tweets surged at the start of songs, while the rate of retweets often fell during musical performances and shot up when BTS was off stage. Content analysis on the subsample found the materials most widely shared were informational or featured concert visuals, mimicking how fans use their phones at in-person concerts. Most original posts received few Retweets and were more personal and expressive of admiration for the performers. Comparison between the samples (concert hashtag vs. stream) also suggests users were strategic in using or omitting official concert hashtags with the strongest differences in the most widely disseminated content. Postings on Twitter during these performances seemed principally directed to fellow fans and audience members, by individuals choosing to share their own excitement and check in with others. By leveraging their existing social media networks, these concert attendees constructed a collective and interactive concert space, connecting with friends and strangers in the crowd and helping each other capture a richer experience than any broadcasting platform currently supports.
Authenticity and presence: defining perceived quality in VR experiences
Authors: Hameed Asim , Perkis Andrew
Published: April 2024

This work expands the existing understanding of quality assessments of VR experiences. Historically, VR quality has focused on presence and immersion, but current discourse emphasizes plausibility and believability as critical for lifelike, credible VR. However, the two concepts are often conflated, leading to confusion. This paper proposes viewing them as subsets of authenticity and presents a structured hierarchy delineating their differences and connections. Additionally, coherence and congruence are presented as complementary quality functions that integrate internal and external logic. The paper considers quality formation in the experience of authenticity inside VR emphasizing that distinguishing authenticity in terms of precise quality features are essential for accurate assessments. Evaluating quality requires a holistic approach across perceptual, cognitive, and emotional factors. This model provides theoretical grounding for assessing the quality of VR experiences.
The relevance of outcome expectations in group hypnosis for stress reduction: a secondary analysis of a multicenter randomized controlled trial
Authors: Siewert Julia , Teut Michael , Brinkhaus Benno , Fisch Silvia , Kummer Sonja
Published: April 2024

Background

There is evidence that patients’ positive outcome expectations prior to study interventions are associated with better treatment outcomes. Nevertheless, to date, only few studies have investigated whether individual outcome expectations affect treatment outcomes in hypnosis.

Objective

To examine whether outcome expectations to hypnosis prior to starting treatment were able to predict perceived stress, as measured on a visual analog scale (VAS), after 5 weeks.

Methods

We performed a secondary data analysis of a multicenter randomized controlled trial of intervention group participants only. Study participants with stress symptoms were randomized to 5 weekly sessions of a group hypnosis program for stress reduction and improved stress coping, plus 5 hypnosis audio recordings for further individual practice at home, as well as an educational booklet on coping with stress. Perceived stress for the following week was measured at baseline and after 5 weeks using a visual analog scale (0–100 mm; VAS). Hypnosis outcome expectations were assessed at baseline only with the Expectations for Treatment Scale (ETS). Unadjusted and adjusted linear regressions were performed to examine the association between baseline expectations and perceived stress at 5 weeks.

Results

Data from 47 participants (M = 45.02, SD = 13.40 years; 85.1% female) were analyzed. Unadjusted (B = 0.326, t = 0.239, p = 0.812, R2 = 0.001) and adjusted (B = 0.639, t = 0.470, p = 0.641, R2 = 0.168) linear regressions found that outcome expectations to hypnosis were not associated with a change in perceived stress between baseline and after 5 weeks in the intervention group.

Conclusion

Our findings suggest that the beneficial effect of group hypnosis in distressed participants were not associated with outcome expectations. Other mechanisms of action may be more important for the effect of hypnosis, which should be explored in future research.

Clinical trial registration: ClinicalTrials.gov, identifier NCT03525093.
Exploring influential factors in the self-assessment of life satisfaction among Chinese elderly: a structural equation modeling analysis
Authors: Yan Jun , Wang Suzhen , Liu Chang , Lu Yuanan
Published: April 2024

The aging problem is becoming more and more prominent globally. Attention to the quality of life and related health improvement among the elderly has become an important issue in modern society. This study utilized a tracking survey conducted in 2017-2018, involving 9,327 Chinese older adults, to examine health influencing factors, and applied structural equation modeling to analyze the influencing factors on the self-assessment of life satisfaction among older adults in different regions (cities, counties, and villages) in China. This study revealed that economic status, psychological status, personal situation, life behaviors, and child care are important influences on older people’s self- assessed life satisfaction. There is a positive correlation between economic status, psychological status, child care and the results of the self-assessment of life satisfaction of the elderly. Psychological status and child care have a greater impact on the self-assessment of life satisfaction among the elderly in urban areas compared to villages and towns. The influence of economic status on the self-assessment of life satisfaction of the elderly is lower in urban areas than in rural areas. There is a significant difference in the influence of personal situations on the self-assessment of life satisfaction among the elderly. Additionally, older individuals tend to report higher levels of self-assessment of life satisfaction. Furthermore, female elderly individuals tend to report higher levels of satisfaction compared to males. Findings from this study indicate that improving health self-assessment in older adults requires targeted efforts based on different geographic areas of life and the age stages of older adults, and more attention needs to be paid to men who are just entering old age.
Neurotoxic lesions of the anterior claustrum influence cued fear memory in rats
Authors: Gu Tengyu , Dong Jing , Ge Jing , Feng Jialu , Liu Xiaoliu , Chen Yun , Liu Jianfeng
Published: April 2024

Background

The claustrum (CLA), a subcortical area between the insular cortex and striatum, innervates almost all cortical regions of the mammalian brain. There is growing evidence that CLA participates in many brain functions, including memory, cognition, and stress response. It is proposed that dysfunction or malfunction of the CLA might be the pathology of some brain diseases, including stress-induced depression and anxiety. However, the role of the CLA in fear memory and anxiety disorders remains largely understudied.

Methods

We evaluated the influences of neurotoxic lesions of the CLA using auditory-cued fear memory and anxiety-like behaviors in rats.

Results

We found that lesions of anterior CLA (aCLA) but not posterior CLA (pCLA) before fear conditioning attenuated fear retrieval, facilitated extinction, and reduced freezing levels during the extinction retention test. Post-learning lesions of aCLA but not pCLA facilitated fear extinction and attenuated freezing behavior during the extinction retention test. Lesions of aCLA or pCLA did not affect anxiety-like behaviors evaluated by the open field test and elevated plus-maze test.

Conclusion

These data suggested that aCLA but not pCLA was involved in fear memory and extinction. Future studies are needed to further investigate the anatomical and functional connections of aCLA subareas that are involved in fear conditioning, which will deepen our understanding of CLA functions.
The association between health behaviours and academic performance moderated by trait mindfulness amongst university students: an observational study
Authors: Heller Sebastian , Reichel Jennifer L. , Mülder Lina M. , Schäfer Markus , Schwab Lisa , Werner Antonia M. , Letzel Stephan , Rigotti Thomas , Dietz Pavel
Published: April 2024

Objectives

To target health communication at less health-conscious groups, evidence on health behaviours’ effects on non-health-related outcomes – such as academic performance – is necessary. Recent research has highlighted the associations of various health behaviours on academic performance of university students. However, there is a lack of research investigating the most predominant health behaviours simultaneously and their association with academic performance, as well as the factors that potentially influence the direction or strength of these associations. Therefore, this study investigated (I) which of the predominant health behaviours (physical activity, healthy diet, sleep, sedentary behaviour, alcohol consumption, smoking, drug use) are most associated with academic performance and (II) whether the personal resource of trait mindfulness moderates these associations.

Methods

An online survey was conducted amongst university students during the 2021 summer semester. Group differences in academic performance regarding health behaviours were analysed using ANOVA (N = 1,049). A first linear regression model (N = 571), considering all selected health behaviours simultaneously, assessed their association with academic performance. A second model (N = 540) assessed interaction effects of health behaviours and trait mindfulness. Separate regressions assessed each interaction’s association with academic performance.

Results

Sleep, fruit and vegetable consumption, and gender were significantly associated with academic performance. The second model showed no significant interaction effects.

Conclusion

Targeting sleep and fruit and vegetable consumption might be the most promising strategies for elevating students’ academic performance, thereby enabling health communication strategies to reach groups driven by performance improvements rather than health benefits.
Pulmonary tuberculosis epidemiology and genetics in Kazakhstan
Authors: Yerezhepov Dauren , Gabdulkayum Aidana , Akhmetova Ainur , Kozhamkulov Ulan , Rakhimova Saule , Kairov Ulykbek , Zhunussova Gulnur , Kalendar Ruslan , Akilzhanova Ainur
Published: April 2024

Background

Tuberculosis (TB) is a major public health emergency in many countries, including Kazakhstan. Despite the decline in the incidence rate and having one of the highest treatment effectiveness in the world, the incidence rate of TB remains high in Kazakhstan. Social and environmental factors along with host genetics contribute to pulmonary tuberculosis (PTB) incidence. Due to the high incidence rate of TB in Kazakhstan, our research aimed to study the epidemiology and genetics of PTB in Kazakhstan.

Materials and methods

1,555 participants were recruited to the case–control study. The epidemiology data was taken during an interview. Polymorphisms of selected genes were determined by real-time PCR using pre-designed TaqMan probes.

Results

Epidemiological risk factors like diabetes (χ2 = 57.71, p < 0.001), unemployment (χ2 = 81.1, p < 0.001), and underweight-ranged BMI (<18.49, χ2 = 206.39, p < 0.001) were significantly associated with PTB. VDR FokI (rs2228570) and VDR BsmI (rs1544410) polymorphisms were associated with an increased risk of PTB. A/A genotype of the TLR8 gene (rs3764880) showed a significant association with an increased risk of PTB in Asians and Asian males. The G allele of the rs2278589 polymorphism of the MARCO gene increases PTB susceptibility in Asians and Asian females. VDR BsmI (rs1544410) polymorphism was significantly associated with PTB in Asian females. A significant association between VDR ApaI polymorphism and PTB susceptibility in the Caucasian population of Kazakhstan was found.

Conclusion

This is the first study that evaluated the epidemiology and genetics of PTB in Kazakhstan on a relatively large cohort. Social and environmental risk factors play a crucial role in TB incidence in Kazakhstan. Underweight BMI (<18.49 kg/m2), diabetes, and unemployment showed a statistically significant association with PTB in our study group. FokI (rs2228570) and BsmI (rs1544410) polymorphisms of the VDR gene can be used as possible biomarkers of PTB in Asian males. rs2278589 polymorphism of the MARCO gene may act as a potential biomarker of PTB in Kazakhs. BsmI polymorphism of the VDR gene and rs2278589 polymorphism of the MARCO gene can be used as possible biomarkers of PTB risk in Asian females as well as VDR ApaI polymorphism in Caucasians.
Human T-lymphotropic virus 1/2 infection among prisoners of a major penitentiary complex of Goiás State, Central-West Brazil
Authors: Okita Michele Tiemi , de Matos Marcos André , Freitas Nara Rúbia de , Silva Ágabo Macedo da Costa e , Marques Juliana Menara de Souza , Marinho Thaís Augusto , Matos Márcia Alves Dias de , Martins Regina Maria Bringel
Published: April 2024

Introduction

Studies on human T-lymphotropic virus 1/2 (HTLV-1/2) infection are scarce in incarcerated population. Therefore, this study estimated the prevalence of HTLV-1/2 infection among prisoners of the major penitentiary complex of Goiás State, Central-West Brazil, comparing it with available data from other Brazilian regions.

Methods

A cross-sectional study was conducted with 910 prisoners of the major penitentiary complex in the State of Goiás, Central-West Brazil. All participants were interviewed, and their serum samples were tested for anti-HTLV-1/2 using an enzyme-linked immunosorbent assay (ELISA; Murex HTLV-I + II, DiaSorin, Dartford, UK). Seropositive samples were submitted for confirmation by a line immunoassay (INNO-LIA HTLV I/II, Fujirebio, Europe N.V., Belgium).

Results

The majority of participants were males (83.1%), between 25 and 39 years old (56.1%; mean age: 31.98 years), self-reported brown ethnicity (56.2%) and reported 9 years or less of formal education (41.4%). Most reported using non-injectable illicit drugs and various sexual behaviors that present risk for sexually transmitted infections (STIs). The prevalence of anti-HTLV-1/2 was 0.33% (95% CI: 0.07–0.96), HTLV-1 (0.22%) and HTLV-2 (0.11%). The two HTLV-1 seropositive prisoners reported high-risk sexual behaviors, and the HTLV-2 seropositive individual was breastfed during childhood (> 6 months) by her mother and three other women.

Conclusion

These data revealed a relatively low seroprevalence of HTLV-1/2 in prisoners in Central-West Brazil, and evidence of HTLV-1 and HTLV-2 circulation in the major penitentiary complex of Goiás State. Given the prevalence of high-risk sexual behaviors, there is a crucial need to intensify education and health programs in prisons to effectively control and prevent HTLV-1/2 and other STIs.
Adherence to antiretroviral therapy and determining factors in adults living with HIV receiving services at public health facilities amidst the COVID-19 crisis in Bahir Dar city, Northwest Ethiopia
Authors: Gela Chalachew Dessie , Tsegaye Gebiyaw Wudie , Shibesh Belayneh Fentahun
Published: April 2024

Background

The COVID-19 pandemic has presented new challenges to adhering to ART, and its influence on adherence and related factors has not been thoroughly studied. This study examines ART adherence and its associated factors during the COVID-19 pandemic.

Methods

A cross-sectional study was conducted on HIV-positive individuals receiving care and treatment in public health facilities. A total of 612 participants were selected using a multi-stage sampling procedure. Data were collected through interviewer-administered questionnaires and chart reviews. We used Epi-data for data entry and Stata for data analysis.

Results

Good adherence to antiretroviral therapy in this study was 76.5% (95% CI, 72.9, 79.7). Divorced marital status (AOR = 0.45,95%CI:0.22,0.90), regular follow-up (AOR = 3.01,95%CI:1.81,5.01), adherence counseling and information in the context of COVID-19 pandemic (AOR = 2.57,95%CI:1.63,4.08), and knowledge about ART (AOR = 1.81,95%CI:1.11,2.94) were significantly associated with adherence to antiretroviral therapy.

Conclusion

The observed adherence rate in this study was lower than the World Health Organization recommendation and previous studies. The study highlighted the importance of addressing adherence to ART among HIV-positive adults during the COVID-19 pandemic and other upcoming emerging and reemerging outbreaks. Strategies to improve adherence should consider factors such as marital status, regular follow-up, provision of counseling and information, and enhancing knowledge about ART.
Experiences of male irregular migrants during their migration process and reception in Spain: lessons learned from the COVID-19 pandemic
Authors: García-López Dulcenombre de María , Jiménez-Lasserrotte María del Mar , Briones-Vozmediano Érica , Ruiz-Fernández María Dolores , Hernández-Padilla José Manuel , Granero-Molina José
Published: April 2024

Background

The causes behind migration movements are complex. The COVID-19 pandemic highlighted how several countries failed to respond to the virus adequately, while simultaneously infringing on people’s rights. Male irregular migrants fled their countries of origin and embarked on a perilous migration journey to Spain. The highly restrictive COVID-19 measures and border closures affected the mobility of male irregular migrants, whose reception in the host country posed a challenge. It led to the establishment of emergency facilities to accommodate male irregular migrants affected by COVID-19, which had repercussions on their mental health. The aim of this study was to describe and understand the experiences of male irregular migrants throughout their migration process and reception in Spain during the COVID-19 pandemic.

Methods

Qualitative descriptive study. Sixteen male irregular migrants participated in this study. Data were collected between January and March 2023 through 16 one-on-one in-depth interviews. Thematic analysis was used to analyze the qualitative data using ATLAS.ti computer software.

Results

Three main themes emerged: (1) How the COVID-19 pandemic drove male irregular migrants to leave their countries of origin, (2) How COVID-19 lockdown policies affected the migration journey, and (3) Receiving male irregular migrants in a pandemic: a housing labyrinth marked by isolation and loneliness.

Conclusion

The COVID-19 pandemic increased the social, employment and health inequalities experienced by male irregular migrants. Border closures exacerbated the migration journey and the social stigmatization of this group, who were seen as carriers of the virus in both transit and host countries. Strict control measures in emergency and reception facilities had a significant psychological impact on the male irregular migrants due to the social isolation they experienced. Health institutions should develop programs to guarantee the care needs of irregular migrants.
Transport from the wild rapidly alters the diversity and composition of skin microbial communities and antifungal taxa in spring peeper frogs
Authors: Kane Lauren P. , Van Bonn William G. , Oliaro Francis J. , Edwardson Christian F. , Smith Malissa , Pinnell Lee J.
Published: April 2024

Amphibians are routinely collected from the wild and added into managed care and public display facilities; however, there is a gap in understanding how these practices might alter the diversity and composition of skin microbial communities on these animals. The aim of this study was to evaluate and compare skin microbial communities of spring peeper frogs (Pseudacris crucifer) from acquisition in the wild through the end of their quarantine period and identify microbial taxa with antifungal properties. From an original group of seventy-six frogs, cohorts of ten were swabbed when acquired in the wild, upon transport from the wild, and swabbed throughout a 9-week quarantine period while under managed care. An immediate loss of microbial richness and diversity was evident upon transfer of the frogs from their original environment and continued throughout subsequent sampling time-points during quarantine. Importantly, antifungal taxa comprised significantly more of the overall skin community after the frogs were moved from the wild, largely due to members of the family Moraxellaceae. Overall, our findings demonstrate that amphibian skin microbiome changes immediately on removal from the wild, and that these changes persist throughout quarantine while being housed under managed care. This may play a pivotal role in the development of dermatological disease and have implications in the health and immune function of amphibians.
Effect of Melatonin on Chemoresistance Exhibited by Spheres Derived from Canine Mammary Carcinoma Cells
Authors: Cataldo Dania , Aravena Guillermo , Escobar Alejandro , Tapia Julio C. , Peralta Oscar A. , Torres Cristian G.
Published: April 2024

Mammary cancer is a frequent disease in female dogs, where a high proportion of cases correspond to malignant tumors that may exhibit drug resistance. Within the mammary tumor microenvironment, there is a cell subpopulation called cancer stem cells (CSCs), which are capable of forming spheres in vitro and resisting anti-tumor treatments, partly explaining the recurrence of some tumors. Previously, it has been described that spheres derived from canine mammary carcinoma cells CF41.Mg and REM 134 exhibit stemness characteristics. Melatonin has shown anti-tumor effects on mammary tumor cells; however, its effects have been poorly evaluated in canine mammary CSCs. This study aimed to analyze the effect of melatonin on the chemoresistance exhibited by stem-like neoplastic cells derived from canine mammary carcinoma to cytotoxic drugs such as doxorubicin and mitoxantrone. CF41.Mg and REM 134 cells were cultured in high-glucose DMEM supplemented with fetal bovine serum and L-glutamine. The spheres were cultured in ultra-low attachment plates in DMEM/F12 medium without fetal bovine serum and with different growth factors. The CD44+/CD24−/low phenotype was analyzed by flow cytometry. The viability of sphere-derived cells (MTS reduction) was studied in the presence of melatonin (0.1 or 1 mM), doxorubicin, mitoxantrone, and luzindole. In addition, the gene (RT-qPCR) of the multidrug resistance bombs MDR1 and ABCG2 were analyzed in the presence of melatonin. Both cell types expressed the MT1 gene, which encodes the melatonin receptor MT1. Melatonin 1 mM does not modify the CD44+/CD24−/low phenotype; however, the hormone reduced viability (p < 0.0001) only in CF41.Mg spheres, without inducing an additive effect when co-incubated with cytotoxic drugs. These effects were independent of the binding of the hormone to its receptor MT1, since, by pharmacologically inhibiting them, the effect of melatonin was not blocked. In CF41.Mg spheres, the relative gene expression of ABCG2 and MDR1 was decreased in response to the hormone (p < 0.001). These results indicate that melatonin negatively modulates the cell survival of spheres derived from CF41.Mg cells, in a way that is independent of its MT1 receptor. These effects did not counteract the resistance to doxorubicin and mitoxantrone, even though the hormone negatively regulates the gene expression of MDR1 and ABCG2.
Characterization of the Worthen Sparrow (Spizella wortheni)’s Nest Building Materials in Northeastern Mexico
Authors: Suarez Eliseo B. , Mellado Miguel , Luna Marcos , Lozano Eloy A. , Calderon Guadalupe , Angel Yesenia , Angel Oscar , Medina Mayra L. , García José E.
Published: April 2024

The study was conducted within a well-managed beef cattle operation in northeastern Mexico. Each nest was weighed and dissected to obtain the plant and animal material used to build the nests. The number of materials present per nest and relative frequency were determined. Twenty-one building materials were used. Over the years, Muhlenbergia torreyi represented 85.5% of the total biomass of the nests, and Aristida longiseta, Bouteloua gracilis, Brickellia canescens, Purshia mexicana and Cirsium ehrenbergii constituted 2.45, 2.80, 2.44, 1.34 and 1.11% of the total biomass, respectively. The above-mentioned grasses represented 95.62% of the total biomass. Material of animal origin was horse and cow hair, which represented 0.84 and 0.58% of the total biomass, respectively. It was concluded that, at the study site, Muhlenbergia torreyi was a key nest-building material for the Worthen sparrow nest.
Study on the Macro-Fine Mechanical Behavior of Ore Flow Based on the Discrete Element Method
Authors: Xia Zhiguo , Deng Zhe , Lu Zengxiang , Ma Chenglong
Published: April 2024

The mechanical behavior associated with the flow of ore-rock bulk materials is an important factor leading to the instability and failure of the shaft wall of the ore storage section in ore passes. It is of great significance for accurately understanding the stability failure characteristics of the shaft wall in the ore storage section in the ore-drawing process to understand the flow characteristics and internal mechanical transfer mechanism of ore-rock bulk. The flow characteristics, contact compactness, stress distribution characteristics, and contact force probability distribution of the ore-rock bulk are analyzed by the discrete element method, which realizes the quantitative characterization of the damage degree of ore-rock flow and reveals the damage mechanism of the shaft wall in the storage section of the ore pass. The results show that (1) in the process of ore-rock particle flow in the ore pass storage section, the macroscopic flow pattern of ore-rock particles changes from a “—” shape to a “V” shape, and the friction between ore-rock particles, particles, and the ore-pass wall is an important reason for the change of the macroscopic flow pattern; (2) the probability distribution of contact force strength between the particles decreases exponentially in the whole ore-drawing process, in which the strong force chains play a major role in the stability of the bulk system; and (3) the overpressure frequency and overpressure coefficient could be used to quantitatively characterize the wall damage degree under the action of ore-rock flow. The dynamic lateral pressure fluctuates periodically in exponential form and decreases, and the dynamic load formed by the ore-rock flow mainly acts on the lower part of the ore storage section.
Study of the Static Characteristics of Gas-Lubricated Thrust Bearings Using Analytical and Finite Element Methods
Authors: Zhang Ke , Zhang Xiaojiao , Zhang Ruiyu
Published: April 2024

A study was conducted to develop a porous aerostatic rectangular thrust bearing model, with the aim of assessing how different operational conditions and geometric factors influence its static capabilities. Initially, the Reynolds equation was analytically solved. Subsequently, simulations were performed on the rectangular air bearing model. Analyzing the impact of throttle hole configurations, air film thickness, orifice size, and supply pressure revealed their significant effect on the bearing’s load capacity, air consumption, peak airflow speed in the air film gap, and rigidity. Experimental validations were further conducted on manufactured bearings, corroborating the theoretical findings. It was observed that extending the length of the rectangular throttle hole array progressively increases gas consumption and diminishes stability, while the load capacity and stiffness initially surge then taper off. A thinner air film enhances load capacity and reduces gas flow, contributing to increased stability. Conversely, enlarging the orifice diameter boosts both load capacity and stability but escalates mass flow and diminishes stiffness. Elevating gas supply pressure enhances load capacity, flow rate, and stiffness, albeit at the cost of reduced stability. A comparative analysis among experimental data, finite element analysis, and analytical solutions showed strong congruence, affirming the precision of the latter two methods for predicting the bearing’s performance. This investigation aids with refining bearing design for precision devices and offers insights to enhance bearing efficiency and lifespan and to reduce friction and wear. Given its lower computational demands, the analytical approach provides a rapid means to assess static characteristics, underscoring its utility alongside finite element techniques for optimizing aerostatic bearing parameters.
Impact of a New Radiation Scheme on Simulated Climate in the Global–Regional Integrated SysTem Model under Varying Physical Parameterization Schemes
Authors: Yuan Chang , Zhang Hua , Jing Xianwen , Zhao Shuyun , Li Xiaohan
Published: April 2024

In this study, the radiation scheme BCC-RAD (Beijing Climate Center RADiative transfer model) developed for global climate models is implemented into the Global–Regional Integrated SysTem (GRIST) model as an alternative to the default RRTMG (general circulation model (GCM) version of the Rapid Radiative Transfer Model) scheme. Its impact on the simulated climate is comprehensively evaluated under different physics parametrization packages, in comparison with both the CERES (partly from ERA5 reanalysis) observations and multi-model results from CMIP6. The results indicate that under the default physics parameterization package of GRIST (PhysC), BCC-RAD improved the simulated global mean cloud cover by ~3% and the clear-sky outgoing longwave radiation by ~5.6 W/m2. Upon the inclusion of the PhysCN parameterization package, BCC-RAD exhibited further improvement in simulated cloud cover and radiative forcing (particularly longwave radiative forcing, the bias of which decreases from −9.2 W/m2 to −1.8 W/m2), leading it to be closer to observations than RRTMG. Additionally, BCC-RAD improved the simulation of atmospheric temperature and hence notably diminished the apparent overestimation of atmospheric humidity seen in RRTMG. This study demonstrates the advantages of BCC-RAD over RRTMG in certain aspects of the GRIST-simulated climate, verifying its capability for the climate-oriented configuration of GRIST.
Influence of Vertical Load, Inflation Pressure, and Driving Speed on the Emission of Tire–Road Particulate Matter and Its Size Distribution
Authors: Schläfle Stefan , Zhang Meng , Unrau Hans-Joachim , Gauterin Frank
Published: April 2024

As fleet electrification progresses, vehicles are continuously becoming heavier, while the used electric motors, with their high torques, enable longitudinal dynamics to be maintained or even increased. This raises the question of what effect electric vehicles have on the emission of tire–road particulate matter (PM). To answer this question, investigations were carried out in this study on a tire internal drum test bench with real road surfaces. In addition to the vertical load, the tire inflation pressure and the driving speed were varied. PM emissions were recorded in real time, resulting in emission factors (emission per kilometer driven) for different load conditions. This allows statements to be made about both the effect on the total emission and on the particle size distribution. It was shown that the PM emission increases linearly with the vertical load at constant longitudinal dynamics. If the tire inflation pressure is increased, the emission also increases linearly, and the increases in emission are equally large for both influences. A clear influence of the driving speed on the emission factor could not be determined. With regard to the particle size distribution, the following correlations were found: higher vertical load and higher tire inflation pressure result in a larger mean particle diameter, while a higher driving speed reduces it. Thus, this study contributes to a better understanding of the expected changes in tire-road PM emissions as a result of electrification.