bluuebunny/crossref_metadata_2025 huggingface dataset:
Selecting Workers Wisely for Crowdsourcing When Copiers and Domain Experts Co-exist
Authors: Fang Xiu , Si Suxin , Sun Guohao , Sheng Quan Z. , Wu Wenjun , Wang Kang , Lv Hang
Published: January 2022

Crowdsourcing integrates human wisdom to solve problems. Tremendous research efforts have been made in this area. However, most of them assume that workers have the same credibility in different domains and workers complete tasks independently. This leads to an inaccurate evaluation of worker credibility, hampering crowdsourcing results. To consider the impact of worker domain expertise, we adopted a vector to more accurately measure the credibility of each worker. Based on this measurement and prior task domain knowledge, we calculated fine-grained worker credibility on each given task. To avoid tasks being assigned to dependent workers who copy answers from others, we conducted copier detection via Bayesian analysis. We designed a crowdsourcing system called SWWC composed of a task assignment stage and a truth discovery stage. In the task assignment stage, we assigned tasks wisely to workers based on worker domain expertise calculation and copier removal. In the truth discovery stage, we computed the estimated truth and worker credibility by an iterative method. Then, we updated the domain expertise of workers to facilitate the upcoming task assignment. We also designed initialization algorithms to better initialize the accuracy of new workers. Theoretical analysis and experimental results showed that our method had a prominent advantage, especially under a copying situation.
A Single-Rate Multicast Congestion Control (SRMCC) Mechanism in Information-Centric Networking
Authors: Duan Yingjie , Ni Hong , Zhu Xiaoyong , Wang Xu
Published: January 2022

Information-centric networking (ICN) is expected to be a candidate for future internet architecture, and it supports features such as multicast that improves bandwidth utilization and transmission efficiency. However, multicast itself does not provide congestion control. When multiple multicast groups coexist, multicast traffic may exhaust all network resources, and cause network congestion and packet loss. Additionally, traditional IP multicast congestion control mechanisms cannot be directly applied to ICN architecture. Therefore, it is necessary to consider an effective congestion control mechanism for ICN multicast. This paper proposes a single-rate multicast congestion control mechanism, called SRMCC. It supports router-assisted awareness of the network congestion state and congestion control message aggregation. Moreover, the fair shared rate estimation method is innovatively proposed to achieve protocol fairness. Most importantly, it adjusts the rate according to different congestion states indicated by the queue occupancy ratio. By introducing a rate selection factor, it can achieve a balance between packet loss rate and throughput. Experimental results show that our proposal outperforms other mechanisms in throughput, packet loss rate, total bandwidth utilization, and overhead, and achieves protocol fairness and better TCP friendliness.
Acknowledgment to Reviewers of Fire in 2021
Authors:   Fire Editorial Office
Published: January 2022

Rigorous peer-reviews are the basis of high-quality academic publishing [...]
Effects of Continuous Light (LD24:0) Modulate the Expression of Lysozyme, Mucin and Peripheral Blood Cells in Rainbow Trout
Authors: Valenzuela Ariel , Rodríguez Ignacia , Schulz Berta , Cortés Raúl , Acosta Jannel , Campos Víctor , Escobar-Aguirre Sebastián
Published: January 2022

Continuous photoperiod is extensively used in fish farming, to regulate the reproductive cycle, despite evidence suggesting that artificial photoperiods can act as a stressor and impair the immune system. We evaluated the potential effects of an artificial photoperiod on mucus components: lysozyme and mucin, in juvenile rainbow trout (Oncorhynchus mykiss) after exposure for one month to natural photoperiod (LD12:12) or constant light (LD24:0) artificial photoperiod. For each treatment, we assessed changes in peripheral blood cells (erythrocytes and leukocytes) and skin mucus component concentrations. Our results show a decrease in lysozyme concentration, while mucin levels are increased. Similarly, we find elevated monocytes and polymorphonuclears under constant light photoperiod. These findings suggest that LD24:0 regulates lysozyme, mucin, and leukocytes, implying that artificial photoperiods could be a stressful.
Crater Depth after the Impact of Multiple Drops into Deep Pools
Authors: Guilizzoni Manfredo , Frontera Giuseppe
Published: January 2022

Many studies have been devoted to single drop impacts onto liquid films and pools, while just a few are available about double drop or drop train impacts, despite the fact that the latter are more realistic situations. Thus, computational fluid dynamics with a volume-of-fluid approach was used here to simulate the impact of multiple drops into deep pools. The aim was to verify if multiple drop impacts significantly differ from single drops ones, and if the models available in the literature for the crater depth in the case of single impacts are reliable also for the multiple drop cases. After validation against experimental data for single and double drop impacts, simulations for four to 30 drops, with a diameter of 2.30 mm, impact velocities 1.0, 1.4, 1.8, and 2.2 m/s, and random initial positions in the domain were performed. The results showed that the time evolution of the crater depth for multiple impacts is similar to the single drop case during the inertial phase, while the following behavior is very different. Consequently, the available models for the maximum crater depth during single drop impacts can still predict the upper and lower bounds of the values of the crater depth during multiple drop impacts within 5% deviation.
Numerical Investigation of Orifice Nearfield Flow Development in Oleo-Pneumatic Shock Absorbers
Authors: Sheikh Al-Shabab Ahmed A. , Grenko Bojan , Vitlaris Dimitrios , Tsoutsanis Panagiotis , Antoniadis Antonios F. , Skote Martin
Published: January 2022

The flow field development through a simplified shock absorber orifice geometry is investigated using a single phase Large Eddy Simulation. Hydraulic oil is used as the working fluid with a constant inlet velocity and an open top boundary to allow the study to focus on the free shear layer and the flow development in the vicinity of the main orifice. The flow field is validated using standard mixing layer dynamics. The impact of the orifice shape is discussed with regards to the initial free shear layer growth, boundary layer development and the potential appearance of cavitation bubbles. Observations are made regarding the presence of flow field disturbances upstream of and through the orifice, thereby, leading to a notable turbulence intensity level in those regions.
Acknowledgment to Reviewers of Fluids in 2021
Authors:   Fluids Editorial Office
Published: January 2022

Rigorous peer-reviews are the basis of high-quality academic publishing [...]
Use of Air-Classification Technology to Manage Mycotoxin and Arsenic Contaminations in Durum Wheat-Derived Products
Authors: Cammerata Alessandro , Marabottini Rosita , Del Frate Viviana , Allevato Enrica , Palombieri Samuela , Sestili Francesco , Stazi Silvia Rita
Published: January 2022

Mycotoxins are the most common natural contaminants and include different types of organic compounds, such as deoxynivalenol (DON) and T-2 and HT-2 toxins. The major toxic inorganic elements include those commonly known as heavy metals, such as cadmium, nickel, and lead, and other minerals such as arsenic. In this study, micronisation and air classification technologies were applied to durum wheat (Triticum turgidum ssp. durum L.) samples to mitigate inorganic (arsenic) and organic contaminants in unrefined milling fractions and final products (pasta). The results showed the suitability of milling plants, providing less refined milling products for lowering amounts of mycotoxins (DON and the sum of T-2 and HT-2 toxins) and toxic inorganic elements (As, Cd, Ni, and Pb). The results showed an As content (in end products) similar to that obtained using semolina as raw material. In samples showing high organic contamination, the contamination rate detected in the more bran-enriched fractions ranged from 74% to 150% (DON) and from 119% to 151% (sum of T2 and HT-2 toxins) as compared to the micronised samples. Therefore, this technology may be useful for manufacturing unrefined products with reduced levels of organic and inorganic contaminants, minimising the health risk to consumers.
Physical Chemical and Textural Characteristics and Sensory Evaluation of Cookies Formulated with Date Seed Powder
Authors: Najjar Zein , Alkaabi Maitha , Alketbi Khulood , Stathopoulos Constantinos , Ranasinghe Meththa
Published: January 2022

Date seeds are a major waste product that can be utilised as a valuable and nutritional material in the food industry. The aim of the present study was to improve cookies quality in terms of functional and textural value and assess the effect of date seed powder flour substitution on the physical and chemical characteristics of cookies. Three substitution levels (2.5, 5 and 7.5%) of flour by fine date seed powder from six varieties locally named Khalas, Khinaizi, Sukkary, Shaham, Zahidi and Fardh were prepared. Two types of flour were used (white flour and whole wheat) at two different baking temperatures: 180 and 200 °C. The incorporation of date seed had no or slight effect on moisture, ash, fat and protein content of the baked cookies. On the other hand, incorporation significantly affected the lightness and hardness of cookies; the higher level of addition, the darker and crispier the resulting cookies. The sensory analysis indicated that the produced cookies were acceptable in terms of smell, taste, texture and overall acceptability. The results indicate that the most acceptable cookies across all evaluated parameters were produced using whole wheat flour with 7.5% levels of date seed powder using Khalas and Zahidi varieties. Overall, the analysis indicated that cookies with acceptable physical characteristics and an improved nutritional profile could be produced with partial replacement of the white/whole wheat flour by date seed powder.
Characterization and Evaluation of Antioxidant and Anti-Inflammatory Activities of Flavonoids from the Fruits of Lycium barbarum
Authors: Yang Tingting , Hu Yuhang , Yan Yamei , Zhou Wangting , Chen Guijie , Zeng Xiaoxiong , Cao Youlong
Published: January 2022

The fruits of Lycium barbarum are rich in flavonoids, which may contribute to the health-promoting function of Lycium barbarum. However, the composition of flavonoids in the fruits of Lycium barbarum (LBFs) has received little attention. Thus, the goal of this work was to identify more kinds of flavonoids from fruits of Lycium barbarum by liquid chromatography–mass spectrometry. The potential antioxidant and anti-inflammatory activities of LBFs in vitro were also investigated. Thirteen flavonoid compounds were identified in LBFs, of which daphnetin, 6,7-dihydroxycoumarin, astragalin, taxifolin, eriodictyol, naringenin, and chrysoeriol were identified for the first time in the fruits of Lycium barbarum, which greatly enriched the variety of flavonoids in the fruits of Lycium barbarum. LBFs showed a similar superior antioxidant activity to vitamin C. Furthermore, LBFs exhibited an anti-inflammatory activity by suppressing the production of nitric oxide and pro-inflammatory cytokines, including tumor necrosis factor-alpha, interleukin-1β, and interleukin-6, in lipopolysaccharide-treated RAW264.7 macrophage cells. This study demonstrated the potential development of LBFs as functional foods.
Antioxidant Activity, Probiotic Survivability, and Sensory Properties of a Phenolic-Rich Pulse Snack Bar Enriched with Lactiplantibacillus plantarum
Authors: Rajagukguk Yolanda Victoria , Arnold Marcellus , Sidor Andrzej , Kulczyński Bartosz , Brzozowska Anna , Schmidt Marcin , Gramza-Michałowska Anna
Published: January 2022

Pulse-based snack bars incorporated with probiotics were developed to provide an overview for the preparation of simple functional food concerning the antioxidant load and iron status improvement. The study focused on the application of microencapsulated probiotics in dry matrices, such as chickpeas and green lentils, in snack bars. The study aims to analyse the products’ antioxidative activities, chemical and sensory properties, as well as the probiotic survivability in the dry matrices. The basic chemical composition showed that 100 g of product can fulfil up to 4.4% and 3.3% of the daily iron value from chickpeas and green lentils, respectively (assuming the iron bioavailability is 23%). Sensory evaluation and hedonic analysis of the fresh pulse snack bar showed that panelists preferred the chickpea snack bar over the green lentil snack bar. For storage analysis, snack bars were stored at 20 °C and were vacuum packaged in sealed low density polyethylene (LDPE) pouches with no light exposure for two months. Hedonic analysis during storage showed significant differences in the aroma of the snack bars (p < 0.05). Generally, the antioxidant activities decreased during the two months of storage. A strong correlation was observed between total phenolic content (TPC) and antioxidant activity assays: ORAC (Oxygen Radical Absorbance Capacity), DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt), PCL (Photochemiluminescence). Moreover, after two months of storage, a 1-log decrease of probiotic viable cells was observed in both snack bars. To meet the dietary requirement of probiotics, it is suggested that people consume five portions and 9.4 portions of the chickpea and green lentil snack bars, respectively. The resulting products have promising properties with respect to probiotics and antioxidant potential in an unconventional way.
In Vitro Potential of Clary Sage and Coriander Essential Oils as Crop Protection and Post-Harvest Decay Control Products
Authors: Raveau Robin , Fontaine Joël , Soltani Abir , Mediouni Ben Jemâa Jouda , Laruelle Frédéric , Lounès-Hadj Sahraoui Anissa
Published: January 2022

Owing to their various application fields and biological properties, natural products and essential oils (EO) in particular are nowadays attracting more attention as alternative methods to control plant pathogens and pests, weeds, and for post-harvest applications. Additionally, to overcome EO stability issues and low persistence of effects, EO encapsulation in β-cyclodextrin (β-CD) could represent a promising avenue. Thus, in this work, the EO distilled from two aromatic plants (Salvia sclarea L. and Coriandrum sativum L.) have been evaluated in vitro for their antifungal, herbicidal and insecticidal activities, against major plant pathogens and pests of agronomical importance. Both plants were grown on unpolluted and trace-element-polluted soils, so as to investigate the effect of the soil pollution on the EO compositions and biological effects. These EO are rich in oxygenated monoterpenes (clary sage and coriander seeds EO), or aliphatic aldehydes (coriander aerial parts EO), and were unaltered by the soil pollution. The tested EO successfully inhibited the growth of two phytopathogenic fungi, Zymoseptoria tritici and Fusarium culmorum, displaying IC50 ranging from 0.46 to 2.08 g L−1, while also exerting anti-germinative, herbicidal, repellent and fumigant effects. However, no improvement of the EO biological effects was observed in the presence of β-CD, under these in vitro experimental conditions. Among the tested EO, the one from aerial parts of coriander displayed the most significant antifungal and herbicidal effects, while the three of them exerted valuable broad-range insecticidal effects. As a whole, these findings suggest that EO produced on polluted areas can be of great interest to the agricultural area, given their faithful chemical compositions and valuable biological effects.
Kinetic Study of Encapsulated β-Carotene Degradation in Aqueous Environments: A Review
Authors: Lavelli Vera , Sereikaitė Jolanta
Published: January 2022

The provitamin A activity of β-carotene is of primary interest to address one of the world’s major malnutrition concerns. β carotene is a fat-soluble compound and its bioavailability from natural sources is very poor. Hence, studies have been focused on the development of specific core/shell micro- or nano-structures that encapsulate β-carotene in order to allow its dispersion in liquid systems and improve its bioavailability. One key objective when developing these structures is also to accomplish β-carotene stability. The aim of this review is to collect kinetic data (rate constants, activation energy) on the degradation of encapsulated β-carotene in order to derive knowledge on the possibility for these systems to be scaled-up to the industrial production of functional foods. Results showed that most of the nano- and micro-structures designed for β-carotene encapsulation and dispersion in the water phase provide better protection with respect to a natural matrix, such as carrot juice, increasing the β-carotene half-life from about 30 d to more than 100 d at room temperature. One promising approach to increase β-carotene stability was found to be the use of wall material, surfactants, or co-encapsulated compounds with antioxidant activity. Moreover, a successful approach was the design of structures, where the core is partially or fully solidified; alternatively, either the core or the interface or the outer phase are gelled. The data collected could serve as a basis for the rational design of structures for β-carotene encapsulation, where new ingredients, especially the extraordinary natural array of hydrocolloids, are applied.
Pulsed Vacuum Drying of Pepper (Capsicum annuum L.): Effect of High-Humidity Hot Air Impingement Blanching Pretreatment on Drying Kinetics and Quality Attributes
Authors: Geng Zhihua , Huang Xiao , Wang Jun , Xiao Hongwei , Yang Xuhai , Zhu Lichun , Qi Xiaochen , Zhang Qian , Hu Bin
Published: January 2022

With a high moisture content, fresh peppers are perishable and rot easily. Drying is essential for shelf-life extension. The natural thin wax layer on the pepper surface hinders moisture transfer. Traditionally, chemical dipping or mechanical pricking is used to remove this wax layer. However, in chemical dipping, chemical residues can trigger food-safety issues, while the low efficiency of mechanical pricking hinders its industrial application. Feasible pretreatment methods are advantageous for industrial use. Here, an emerging pretreatment technique (high-humidity hot-air impingement blanching, HHAIB) was used for peppers before drying and its effects on drying characteristics, microstructure, and polyphenol oxidase (PPO) activity were explored. The impact of drying temperature on color parameters and red pigment content of pulsed-vacuum-dried peppers was also evaluated. PPO activity was reduced to less than 20% after blanching at 110 °C for 60 s. HHAIB reduced drying time and PPO activity and promoted chemical-substance release. Effective water diffusivity was highest (5.01 × 10−10 m2/s) after blanching at 110 °C for 90 s, and the brightness value and red pigment content were highest (9.94 g/kg) at 70 °C. HHAIB and pulsed vacuum drying are promising pretreatment and drying methods for enhancing the drying rate and quality of red peppers.
A Comprehensive Review on Bio-Preservation of Bread: An Approach to Adopt Wholesome Strategies
Authors: Rahman Mizanur , Islam Raihanul , Hasan Shariful , Zzaman Wahidu , Rana Md Rahmatuzzaman , Ahmed Shafi , Roy Mukta , Sayem Asm , Matin Abdul , Raposo António , Zandonadi Renata Puppin , Botelho Raquel Braz Assunção , Sunny Atiqur Rahman
Published: January 2022

Bread is a food that is commonly recognized as a very convenient type of food, but it is also easily prone to microbial attack. As a result of bread spoilage, a significant economic loss occurs to both consumers and producers. For years, the bakery industry has sought to identify treatments that make bread safe and with an extended shelf-life to address this economic and safety concern, including replacing harmful chemical preservatives. New frontiers, on the other hand, have recently been explored. Alternative methods of bread preservation, such as microbial fermentation, utilization of plant and animal derivatives, nanofibers, and other innovative technologies, have yielded promising results. This review summarizes numerous research findings regarding the bio-preservation of bread and suggests potential applications of these techniques. Among these techniques, microbial fermentation using lactic acid bacteria strains and yeast has drawn significant interest nowadays because of their outstanding antifungal activity and shelf-life extending capacity. For example, bread slices with Lactobacillus plantarum LB1 and Lactobacillus rossiae LB5 inhibited fungal development for up to 21 days with the lowest contamination score. Moreover, various essential oils and plant extracts, such as lemongrass oil and garlic extracts, demonstrated promising results in reducing fungal growth on bread and other bakery products. In addition, different emerging bio-preservation strategies such as the utilization of whey, nanofibers, active packaging, and modified atmospheric packaging have gained considerable interest in recent days.
Antibody Generation and Rapid Immunochromatography Using Time-Resolved Fluorescence Microspheres for Propiconazole: Fungicide Abused as Growth Regulator in Vegetable
Authors: Chen Bo , Shen Xing , Li Zhaodong , Wang Jin , Li Xiangmei , Xu Zhenlin , Shen Yudong , Lei Yi , Huang Xinan , Wang Xu , Lei Hongtao
Published: January 2022

Propiconazole (PCZ) is a fungicide popularly used to prevent and control wheat and rice bakanae disease, etc. However, it was recently found to be illegally employed as a plant regulator to induce thick stems and dark green leaves of Brassica campestris, a famous vegetable in Guangdong, South China. Due to a lack of available recognition molecules to the target analyte, it is still a big challenge to establish a rapid surveillance screening method. In this study, a novel chiral hapten was rationally designed, and an artificial immunogen was then prepared for the generation of a specific antibody against propiconazole for the first time. Using the obtained antibody, a highly sensitive time-resolved fluorescence microspheres lateral flow immunochromatographic assay (TRFMs-LFIA) was established with a visual limit of detection of 100 ng/mL and a quantitative limit of detection of 1.92 ng/mL for propiconazole. TRFMs-LFIA also exhibited good recoveries ranging from 78.6% to 110.7% with coefficients of variation below 16%. The analysis of blind real-life samples showed a good agreement with results obtained using HPLC-MS/MS. Therefore, the proposed method could be used as an ideal screening surveillance tool for the detection of propiconazole in vegetables.
The Effects of Antioxidants and Packaging Methods on Inhibiting Lipid Oxidation in Deep Fried Crickets (Gryllus bimaculatus) during Storage
Authors: Gan Jin , Zhao Min , He Zhao , Sun Long , Li Xian , Feng Ying
Published: January 2022

This study aimed to investigate the effect of processing methods on inhibiting lipid oxidation of deep fried crickets (Gryllus bimaculatus) during storage. Four antioxidants and two packaging methods were used. The effects of different antioxidants and packaging methods on composition of fatty acids, contents of free fatty acids (FFA), peroxide value (PV), and thiobarbituric acid reactive substances (TBARSs) value of deep fried Gryllus bimaculatus were analyzed during 150 days of storage. The composition of fatty acids changed and the content of FFA, PV, and TBARs value also increased with the extension of storage time, indicating that the lipid oxidation dominated by oxidation of unsaturated fatty acids could occur in deep fried Gryllus bimaculatus during storage. In the same storage period, the total content of FFA, PV, and TBARs value of samples treated with antioxidants and vacuum-filling nitrogen packaging were lower than those of controls, suggesting that antioxidants and vacuum-filling nitrogen packaging have noticeable effects on inhibiting lipid oxidation and improving the quality of deep fried crickets, and dibutyl hydroxyl toluene (BHT) was found as the most effective antioxidant in this study. The results may provide a reliable reference for processing of deep fried edible insects.
A Bayesian Approach to Predict Food Fraud Type and Point of Adulteration
Authors: Soon Jan Mei , Abdul Wahab Ikarastika Rahayu
Published: January 2022

Primary and secondary food processing had been identified as areas vulnerable to fraud. Besides the food processing area, other stages within the food supply chain are also vulnerable to fraud. This study aims to develop a Bayesian network (BN) model to predict food fraud type and point of adulteration i.e., the occurrence of fraudulent activity. The BN model was developed using GeNie Modeler (BayesFusion, LLC) based on 715 notifications (1979–2018) from Food Adulteration Incidents Registry (FAIR) database. Types of food fraud were linked to six explanatory variables such as food categories, year, adulterants (chemicals, ingredients, non-food, microbiological, physical, and others), reporting country, point of adulteration, and point of detection. The BN model was validated using 80 notifications from 2019 to determine the predictive accuracy of food fraud type and point of adulteration. Mislabelling (20.7%), artificial enhancement (17.2%), and substitution (16.4%) were the most commonly reported types of fraud. Beverages (21.4%), dairy (14.3%), and meat (14.0%) received the highest fraud notifications. Adulterants such as chemicals (21.7%) (e.g., formaldehyde, methanol, bleaching agent) and cheaper, expired or rotten ingredients (13.7%) were often used to adulterate food. Manufacturing (63.9%) was identified as the main point of adulteration followed by the retailer (13.4%) and distribution (9.9%).
Incorporation of Limosilactobacillus fermentum UCO-979C with Anti-Helicobacter pylori and Immunomodulatory Activities in Various Ice Cream Bases
Authors: Paucar-Carrión Cristina , Espinoza-Monje Marcela , Gutiérrez-Zamorano Cristian , Sánchez-Alonzo Kimberly , Carvajal Romina I. , Rogel-Castillo Cristian , Sáez-Carrillo Katia , García-Cancino Apolinaria
Published: January 2022

Limosilactobacillus fermentum UCO-979C is a probiotic strain possessing anti-Helicobacter pylori and immunomodulatory activity. The aim of this work was to examine if this strain maintains its probiotic properties and its viability when added to dairy-based ice creams (cookies and cream, Greek yogurt, and chocolate with brownie) or to fruit-based ice creams (pineapple and raspberry) stored at −18 °C for 90 days. The probiotic anti-H. pylori activity using the well diffusion test, its immunomodulatory activity was measured using transforming growth factor beta 1 (TGF-β1) cytokine production by human gastric adenocarcinoma (AGS) cells, and its viability was measured using the microdrop technique. Assays were performed in triplicate. The L. fermentum UCO-979C strain maintained strong anti-H. pylori activity in dairy-based ice creams and mild activity in fruit-based ice cream. The production of pro-inflammatory cytokine TGF-β1 on AGS cells was higher in the probiotic recovered from Greek yogurt ice cream, maintaining a viability exceeding 107 colony-forming units/mL. The addition of the probiotic to ice creams did not significantly influence the physicochemical properties of the product. These data show the great potential of the L. fermentum UCO-979C strain in producing probiotic dairy-based and fruit-based ice creams.
Preparation of an Immunoaffinity Column Based on Bispecific Monoclonal Antibody for Aflatoxin B1 and Ochratoxin A Detection Combined with ic-ELISA
Authors: Lu Disha , Wang Xu , Su Ruijue , Cheng Yongjian , Wang Hong , Luo Lin , Xiao Zhili
Published: January 2022

A novel and efficient immunoaffinity column (IAC) based on bispecific monoclonal antibody (BsMAb) recognizing aflatoxin B1 (AFB1) and ochratoxin A (OTA) was prepared and applied in simultaneous extraction of AFB1 and OTA from food samples and detection of AFB1/OTA combined with ic-ELISA (indirect competitive ELISA). Two deficient cell lines, hypoxanthine guanine phosphoribosyl-transferase (HGPRT) deficient anti-AFB1 hybridoma cell line and thymidine kinase (TK) deficient anti-OTA hybridoma cell line, were fused to generate a hybrid-hybridoma producing BsMAb against AFB1 and OTA. The subtype of the BsMAb was IgG1 via mouse antibody isotyping kit test. The purity and molecular weight of BsMAb were confirmed by SDS-PAGE method. The cross-reaction rate with AFB2 was 37%, with AFG1 15%, with AFM1 48%, with AFM2 10%, and with OTB 36%. Negligible cross-reaction was observed with other tested compounds. The affinity constant (Ka) was determined by ELISA. The Ka (AFB1) and Ka (OTA) was 2.43 × 108 L/mol and 1.57 × 108 L/mol, respectively. Then the anti-AFB1/OTA BsMAb was coupled with CNBr-Sepharose, and an AFB1/OTA IAC was prepared. The coupling time and elution conditions of IAC were optimized. The coupling time was 1 h with 90% coupling rate, the eluent was methanol–water (60:40, v:v, pH 2.3) containing 1 mol/L NaCl, and the eluent volume was 4 mL. The column capacities of AFB1 and OTA were 165.0 ng and 171.3 ng, respectively. After seven times of repeated use, the preservation rates of column capacity for AFB1 and OTA were 69.3% and 68.0%, respectively. The ic-ELISA for AFB1 and OTA were applied combined with IAC. The IC50 (50% inhibiting concentration) of AFB1 was 0.027 ng/mL, the limit of detection (LOD) was 0.004 ng/mL (0.032 µg/kg), and the linear range was 0.006 ng/mL~0.119 ng/mL. The IC50 of OTA was 0.878 ng/mL, the LOD was 0.126 ng/mL (1.008 µg/kg), and the linear range was 0.259 ng/mL~6.178 ng/mL. Under optimum conditions, corn and wheat samples were pretreated with AFB1-OTA IAC. The recovery rates of AFB1 and OTA were 95.4%~105.0% with ic-ELISA, and the correlations between the detection results and LC-MS were above 0.9. The developed IAC combined with ic-ELISA is reliable and could be applied to the detection of AFB1 and OTA in grains.
An Active Peptide-Based Packaging System to Improve the Freshness and Safety of Fish Products: A Case Study
Authors: Ambrosio Rosa Luisa , Gogliettino Marta , Agrillo Bruna , Proroga Yolande T. R. , Balestrieri Marco , Gratino Lorena , Cristiano Daniela , Palmieri Gianna , Anastasio Aniello
Published: January 2022

Fresh fish are highly perishable, owing mainly to their moisture content, high amount of free amino acids and polyunsaturated fatty acids. Microorganisms and chemical reactions cause the spoilage, leading to loss in quality, human health risks and a market value reduction. Therefore, the fishing industry has always been willing to explore new technologies to increase quality and safety of fish products through a decrease of the microbiological and biochemical damage. In this context, antimicrobial active packaging is one such promising solution to meet consumer demands. The main objective of this study was to evaluate the effects of an active polypropylene-based packaging functionalized with the antimicrobial peptide 1018K6 on microbial growth, physicochemical properties and the sensory attributes of raw salmon fillets. The results showed that application of 1018K6-polypropylene strongly inhibited the microbial growth of both pathogenic and specific spoilage organisms (SSOs) on fish fillets after 7 days. Moreover, salmon also kept its freshness as per volatile chemical spoilage indices (CSIs) during storage. Similar results were obtained on hamburgers of Sarda sarda performing the same analyses. This work provides further evidence that 1018K6-polymers have good potential as antimicrobial packaging for application in the food market to enhance quality and preserve the sensorial properties of fish products.
Stone Milling versus Roller Milling in Soft Wheat (Part 2): Influence on Nutritional and Technological Quality of Products
Authors: Carcea Marina , Narducci Valentina , Turfani Valeria , Finotti Enrico
Published: January 2022

Wholegrain soft wheat flours can be obtained by either roller milling or stone milling. In this paper, we report on the continuation of a study aimed at analysing compositional and technological differences between differently milled wholegrain flours. Eight mixes of soft wheat grains were stone milled and roller milled and the milling products analysed for their phytic acid, lipids composition to determine the presence of trans-fatty acids and damaged starch. A wholegrain flour milled with a laboratory disk mill was also analysed as comparison, as well as seven wholegrain flours purchased on the market. For phytic acid we found that that there is no compositional difference between a stone milled or a roller milled flour if the milling streams are all recombined: the milling streams instead have different amounts of phytic acid which is mainly present in the fine bran and coarse bran. It was not possible to highlight differences in the milling technology due to the presence of trans-fatty acids in the stone milled wholegrain flour whereas it was possible to find that starch damage depended on the milling method with stone milled wholegrain flours having in all cases significantly higher values than the roller milled ones.
NMR Metabonomic Profile of Preterm Human Milk in the First Month of Lactation: From Extreme to Moderate Prematurity
Authors: Peila Chiara , Sottemano Stefano , Cesare Marincola Flaminia , Stocchero Matteo , Pusceddu Nicoletta Grazia , Dessì Angelica , Baraldi Eugenio , Fanos Vassilios , Bertino Enrico
Published: January 2022

Understanding the composition of human milk (HM) can provide important insights into the links between infant nutrition, health, and development. In the present work, we have longitudinally investigated the metabolome of milk from 36 women delivering preterm at different gestational ages (GA): extremely (<28 weeks GA), very (29–31 weeks GA) or moderate (32–34 weeks GA) premature. Milk samples were collected at three lactation stages: colostrum (3–6 days post-partum), transitional milk (7–15 days post-partum) and mature milk (16–26 days post-partum). Multivariate and univariate statistical data analyses were performed on the 1H NMR metabolic profiles of specimens in relation to the degree of prematurity and lactation stage. We observed a high impact of both the mother’s phenotype and lactation time on HM metabolome composition. Furthermore, statistically significant differences, although weak, were observed in terms of GA when comparing extremely and moderately preterm milk. Overall, our study provides new insights into preterm HM metabolome composition that may help to optimize feeding of preterm newborns, and thus improve the postnatal growth and later health outcomes of these fragile patients.
Glucosinolates, Ca, Se Contents, and Bioaccessibility in Brassica rapa Vegetables Obtained by Organic and Conventional Cropping Systems
Authors: Cámara-Martos Fernando , Obregón-Cano Sara , de Haro-Bailón Antonio
Published: January 2022

In northwest Spain and Portugal, there is a long tradition of cultivating B. rapa subsp. rapa to obtain turnip greens and turnip tops. Brassica rapa L. subsp. rapa (turnip greens and turnip tops) were grown under conventional and organic conditions in two Farms in southern Spain. Glucosinolatescontents were higher in Brassicas grown under conventional conditions than those grown under organic ones. Average Ca total and bioaccessible contents ranged between 14.6–23.4 mg/g; 8.9–12.0 mg/g for turnip greens and 6.4–8.9 mg/g; 4.3–4.8 mg/g for turnip tops. According to these concentrations, an intake of 100–200 g (fresh weight) of the studied Brassica rapa fulfills Ca dietary reference intakes (DRI) (considering the total content data) and complies with 72–100% Ca DRI percentage (considering the bioaccessible data). Se concentrations ranged between 0.061–0.073 µg/g and 0.039–0.053 µg/g for turnip greens and turnip tops respectively. Se bioaccessibility values were high, with percentages of around 90%. Finally, the total glucosinolate content ranged between 13.23–21.28 µmol/g for turnip greens and 13.36–20.20 µmol/g for turnip tops. In general, the bioaccessibility of the total glucosinolates analyzed in this study was high, with mean values of around 73% and 66% for turnip greens and turnip tops, respectively. Brassica rapa vegetables grown under both organic and conventional conditions in southern Spain are an excellent dietary source of Ca, Se, and glucosinolates with a high bioaccessibility.
Update on Biogenic Amines in Fermented and Non-Fermented Beverages
Authors: Visciano Pierina , Schirone Maria
Published: January 2022

The formation of biogenic amines in food and beverages is mainly due to the presence of proteins and/or free amino acids that represent the substrates for microbial or natural enzymes with decarboxylation or amination activity. Fermentation occurring in many alcoholic beverages, such as wine, beer, cider, liqueurs, as well as coffee and tea, is one of the main processes affecting their production. Some biogenic amines can also be naturally present in some fruit juices or fruit-based drinks. The dietary intake of such compounds should consider all their potential sources by both foods and drinks, taking in account the health impact on some consumers that represent categories at risk for a deficient metabolic activity or assuming inhibiting drugs. The most important tool to avoid their adverse effects is based on prevention through the selection of lactic acid bacteria with low decarboxylating activity or good manufacturing practices hurdling the favoring conditions on biogenic amines’ production.
An Evaluation of a Virtual Food Safety Program for Low-Income Families: Applying the Theory of Planned Behavior
Authors: Archila-Godínez Juan C. , Chen Han , Klinestiver Leah , Rosa Lia , Barrett Tressie , Henley Shauna C. , Feng Yaohua
Published: January 2022

Low-income families are reported to have a limited knowledge of food safety and resources to follow food safety practices compared with the rest of the population. This paper evaluated a virtual food safety educational program targeting food handlers in low-income families. Trained native speakers of English and Spanish delivered course materials in both languages. A total of 60 individuals participated in the program, with 30 participants in each language group. Most were female, and most had fewer than three children. After the program, participants’ food safety knowledge and self-reported safe food practice behavior scores increased significantly from 5.32 to 7.43 (out of 8.00) and from 24.78 to 29.30 (out of 35.00), respectively. The theory of planned behavior (TPB) was used to understand individuals’ behavior change intention of food safety practices. All the TPB constructs’ scores, including attitudes toward the behavior, perceived behavioral control, subjective norms, and behavior change intentions, were improved significantly; however, only the subjective norms and perceived behavioral control were significantly correlated with the behavior change intentions. This virtual educational program improved low-income individuals’ food safety knowledge and changed their food safety attitudes and behaviors, giving a path to develop and evaluate more virtual food safety educational programs in the future.
High Prevalence of Enterobacterales in the Smear of Surface-Ripened Cheese with Contribution to Organoleptic Properties
Authors: Ritschard Jasmine S. , Van Loon Hanne , Amato Lea , Meile Leo , Schuppler Markus
Published: January 2022

The smear of surface-ripened cheese harbors complex microbiota mainly composed of typical Gram-positive aerobic bacteria and yeast. Gram-negative bacteria are usually classified as un-wanted contaminants. In order to investigate the abundance and impact of Gram-negative bacte-ria naturally occurring in the smear of surface-ripened cheese, we performed a culture-based analysis of smear samples from 15 semi-hard surface-ripened cheese varieties. The quantity, di-versity and species distribution of Proteobacteria in the surface smear of the analyzed cheese vari-eties were unexpectedly high, and comprised a total of 22 different species. Proteus and Morganella predominated most of the analyzed cheese varieties, while Enterobacter, Citrobacter, Hafnia and Serratia were also found frequently. Further physiological characterization of Proteus isolates re-vealed strong proteolytic activity, and the analysis of volatiles in the smear cheese surface head-space suggested that Enterobacterales produce volatile organic flavor compounds that contribute to the organoleptic properties of surface-ripened cheese. Autochthonous members of Enterobac-terales were found in 12 of the 15 smear samples from surface-ripened cheeses, suggesting that they are part of the typical house microbiota that shape the organoleptic properties of the cheese rather than represent unwanted contaminants. However, further investigation on safety issues of the individual species should be performed in order to manage the health risk for consumers.
Short Term Electric Power Load Forecasting Using Principal Component Analysis and Recurrent Neural Networks
Authors: Veeramsetty Venkataramana , Chandra Dongari Rakesh , Grimaccia Francesco , Mussetta Marco
Published: January 2022

Electrical load forecasting study is required in electric power systems for different applications with respect to the specific time horizon, such as optimal operations, grid stability, Demand Side Management (DSM) and long-term strategic planning. In this context, machine learning and data analytics models represent a valuable tool to cope with the intrinsic complexity and especially design future demand-side advanced services. The main novelty in this paper is that the combination of a Recurrent Neural Network (RNN) and Principal Component Analysis (PCA) techniques is proposed to improve the forecasting capability of the hourly load on an electric power substation. A historical dataset of measured loads related to a 33/11 kV MV substation is considered in India as a case study, in order to properly validate the designed method. Based on the presented numerical results, the proposed approach proved itself to accurately predict loads with a reduced dimensionality of input data, thus minimizing the overall computational effort.
Analytical Study of a ϕ− Fractional Order Quadratic Functional Integral Equation
Authors: El-Sayed Ahmed M. A. , Hashem Hind H. G. , Al-Issa Shorouk M.
Published: January 2022

Quadratic integral equations of fractional order have been studied from different views. Here we shall study the existence of continuous solutions of a ϕ− fractional-orders quadratic functional integral equation, establish some properties of these solutions and prove the existence of maximal and minimal solutions of that quadratic integral equation. Moreover, we introduce some particular cases to illustrate our results.
Hermite-Hadamard Fractional Inequalities for Differentiable Functions
Authors: Samraiz Muhammad , Perveen Zahida , Rahman Gauhar , Adil Khan Muhammad , Nisar Kottakkaran Sooppy
Published: January 2022

In this article, we look at a variety of mean-type integral inequalities for a well-known Hilfer fractional derivative. We consider twice differentiable convex and s-convex functions for s∈(0,1] that have applications in optimization theory. In order to infer more interesting mean inequalities, some identities are also established. The consequences for Caputo fractional derivative are presented as special cases to our general conclusions.
Fractional Order Modeling the Gemini Virus in Capsicum annuum with Optimal Control
Authors: Nisar Kottakkaran Sooppy , Logeswari Kumararaju , Vijayaraj Veliappan , Baskonus Haci Mehmet , Ravichandran Chokkalingam
Published: January 2022

In this article, a fractional model of the Capsicum annuum (C. annuum) affected by the yellow virus through whiteflies (Bemisia tabaci) is examined. We analyzed the model by equilibrium points, reproductive number, and local and global stability. The optimal control methods are discussed to decrease the infectious B. tabaci and C. annuum by applying the Verticillium lecanii (V. lecanii) with the Atangana–Baleanu derivative. Numerical results described the population of plants and comparison values of using V. lecanni. The results show that using 60% of V. lecanni will control the spread of the yellow virus in infected B. tabaci and C. annuum in 10 days, which helps farmers to afford the costs of cultivating chili plants.
Existence and Uniqueness of Mild Solution Where α ∈ (1,2) for Fuzzy Fractional Evolution Equations with Uncertainty
Authors: Shafqat Ramsha , Niazi Azmat Ullah Khan , Jeelani Mdi Begum , Alharthi Nadiyah Hussain
Published: January 2022

This paper concerns with the existence and uniqueness of fuzzy fractional evolution equation with uncertainty involves function of form cDαx(t)=f(t,x(t),Dβx(t)),Iαx(0)=x0,x′(0)=x1, where 1<α<2,0<β<1. After determining the equivalent integral form of solution we establish existence and uniqueness by using Rogers conditions, Kooi type conditions and Krasnoselskii-Krein type conditions. In addition, various numerical solutions have been presented to ensure that the main result is true and effective. Finally, a few examples which express fuzzy fractional evolution equations are shown.
Effect of Humidity and Temperature on the Impedances and Voltage of Al/Gr-Jelly/Cu-Rubber Composite-Based Flexible Electrochemical Sensors
Authors: Chani Muhammad Tariq Saeed , Karimov Khasan S. , Bakhsh Esraa M. , Rahman Mohammed M.
Published: January 2022

Here we present the fabrication of graphene and jelly (superabsorbent polymer) electrolyte composite-based shockproof flexible electrochemical sensors (Al/Gr-Jelly/Cu) and their properties under the effect of humidity and temperature. A layer of graphene mixed in jelly electrolyte was drop-casted onto porous rubber substrates between preliminary fixed aluminum (Al) and copper (Cu) electrodes followed by rubbing-in. It was observed that the graphene and jelly mixture was mechanically soft and flexible, similar to jelly. Electrically, this mixture (graphene and jelly) behaved as a flexible electrolyte. It was observed that under the effect of humidity ranging from 47 to 98%, the impedances of the sensors decreased by 2.0 times on average. Under the effect of temperatures ranging from 21 to 41 °C the impedances decreased by 2.4 times. The average temperature coefficient of impedances was equal to −0.03 °C−1. The electrochemical voltage generated by the flexible jelly electrolyte sensors was also investigated. It was found that the initial open-circuit voltages were equal to 201 mV and increased slightly, by 5–10% under the effect of humidity and temperature as well. The short-circuit currents under the effect of humidity and temperature increased by 2–3 times. The Al/Gr-Jelly/Cu electrochemical sensors may be used as prototypes for the development of the jelly electronic-based devices.
Improved Dose Response of N-(hydroxymethyl)acrylamide Gel Dosimeter with Calcium Chloride for Radiotherapy
Authors: Rabaeh Khalid A. , Al-Tarawneh Rawan E. , Eyadeh Molham M. , Hammoudeh Issra’ M. E. , Shatnawi Moneeb T. M.
Published: January 2022

The impact of calcium chloride (CaCl2) on the performance of N-(hydroxymethyl)acrylamide (NHMA) polymer gel dosimeter is studied in this article. The dosimeter was exposed to doses of up to 10 Gy with radiation beam-energy of 10 MV and dose-rates of 300 cGy/min. The relaxation rate (R2) parameter was utilized to explore the performance of irradiated NHMAGAT gels. The dose response in terms of R2 increased from 0.29 to 0.63 Gy−1·s−1 with increasing calcium chloride concentration from 0 to 1000 mM. The results show no substantial impact of dose-rates as well as radiation energies on NHMAGAT samples. For the steadiness of irradiated NHMAGAT dosimeters, it was found that there is no apparent variation in R2 (less than ±3%; standard deviation) up to 3 days. The overall uncertainty of the gel dosimeter with calcium chloride is 4.96% (double standard deviation, 95% confidence level).