bluuebunny/crossref_metadata_2025 huggingface dataset:
Streamflow Estimation through Coupling of Hieararchical Clustering Analysis and Regression Analysis—A Case Study in Euphrates-Tigris Basin
Authors: Guzey Goksel Ezgi , Onoz Bihrat
Published: July 2023

In this study, the resilience of designed water systems in the face of limited streamflow gauging stations and escalating global warming impacts were investigated. By performing a regression analysis, simulated meteorological data with observed streamflow from 1971 to 2020 across 33 stream gauging stations in the Euphrates-Tigris Basin were correlated. Utilizing the Ordinary Least Squares regression method, streamflow for 2020–2100 using simulated meteorological data under RCP 4.5 and RCP 8.5 scenarios in CORDEX-EURO and CORDEX-MENA domains were also predicted. Streamflow variability was calculated based on meteorological variables and station morphological characteristics, particularly evapotranspiration. Hierarchical clustering analysis identified two clusters among the stream gauging stations, and for each cluster, two streamflow equations were derived. The regression analysis achieved robust streamflow predictions using six representative climate variables, with adj. R2 values of 0.7–0.85 across all models, primarily influenced by evapotranspiration. The use of a global model led to a 10% decrease in prediction capabilities for all CORDEX models based on R2 performance. This study emphasizes the importance of region homogeneity in estimating streamflow, encompassing both geographical and hydro-meteorological characteristics.
BDE-47 Induces Mitochondrial Dysfunction and Endoplasmic Reticulum Stress to Inhibit Early Porcine Embryonic Development
Authors: Liu Rong-Ping , He Sheng-Yan , Wang Jing , Wang Xin-Qin , Jin Zhe-Long , Guo Hao , Wang Chao-Rui , Xu Yong-Nan , Kim Nam-Hyung
Published: July 2023

Widely used as a flame retardant, 2,2′4,4′-tetrabromodiphenyl ether (BDE-47) is a persistent environmental pollutant with toxicological effects, including hepatotoxicity, neurotoxicity, reproductive toxicity, and endocrine disruption. To investigate the toxicological effects of BDE-47 on early porcine embryogenesis in vitro, cultured porcine embryos were exposed to BDE-47 during early development. Exposure to 100 μM BDE-47 decreased the blastocyst rate and mRNA level of pluripotency genes but increased the level of LC3 and the expression of autophagy-related genes. After BDE-47 exposure, porcine embryos’ antioxidant capability decreased; ROS levels increased, while glutathione (GSH) levels and the expression of antioxidant-related genes decreased. In addition, BDE-47 exposure reduced mitochondrial abundance and mitochondrial membrane potential levels, downregulated mitochondrial biogenesis-associated genes, decreased endoplasmic reticulum (ER) abundance, increased the levels of GRP78, a marker of ER stress (ERS), and upregulated the expression of ERS-related genes. However, ER damage and low embryo quality induced by BDE-47 exposure were reversed with the ERS inhibitor, the 4-phenylbutyric acid. In conclusion, BDE-47 inhibits the development of early porcine embryos in vitro by inducing mitochondrial dysfunction and ERS. This study sheds light on the mechanisms of BDE-47-induced embryonic toxicity.
Agonic Aspiration of Blood: Not Useful as an Animal-Based Indicator of Electrical Stunning Ineffectiveness in Pigs (Sus scrofa domesticus)
Authors: Ferreira Maria Francisca , Fàbrega Emma , Pires Isabel , Vieira-Pinto Maria Madalena
Published: July 2023

Agonic aspiration of blood (AAB) may result from an inadequate exsanguination with accidental trachea severing, that can be favoured by ineffective stunning of pigs (Sus scrofa domesticus). This study aimed to evaluate AAB as an animal-based indicator of electrical stunning ineffectiveness, which could be used by official veterinarians during the post-mortem inspection of pigs. Information on 3584 finishing pigs was collected at a Portuguese abattoir that performs head-to-body electrical stunning with horizontal bleeding. Of them, 15.5% of the pigs presented signs of ineffective stunning. AAB lung lesions were found in 27.8% of lungs. Despite what was predicted, a strong correlation was found between well stunned animals and the presence of blood lesions in lungs (p = 0.006). Statistical significances were found between pigs’ lateral recumbency at the conveyor and the presence of blood affecting one lung. Under the conditions of this study, the authors cannot point to AAB lung lesions as an animal-based indicator of electrical stunning ineffectiveness. Further studies should be conducted to establish a better understanding of the causes of aspiration of blood, in particular how horizontal bleeding may affect the occurrence of similar lesions.
Diversity and Co-Occurrence Pattern Analysis of Cecal and Jejunal Microbiota in Two Rabbit Breeds
Authors: Abdel-Kafy El-Sayed M. , Kamel Kamel I. , Severgnini Marco , Morsy Shama H. A. , Cremonesi Paola , Ghoneim Shereen S. , Brecchia Gabriele , Ali Neama I. , Abdel-Ghafar Yasmein Z. , Ali Wael A. H. , Shabaan Hoda M. A.
Published: July 2023

This study aimed to evaluate the productive performance and microbiota variation in the jejunum and cecum of two rabbit breeds with different growth rates. This study was carried out on Native Middle-Egypt Breed (NMER) and Giant Flanders (GF) rabbits from 5 weeks to 12 weeks of age. Twenty NMER (NM) and GF male rabbits were slaughtered, and the jejunum and cecum tracts were collected to assay gut microbiota composition via 16S ribosomal RNA (rRNA) gene sequencing and histology examination. At 12 weeks of age, daily weight gain, villus height in the jejunum, total protein, and albumin were higher in GF rabbits than in NMER rabbits. Also, the jejunal villi of GF were well arranged in their dense borders. The microbiota between the jejunum and cecum was significantly different in terms of Beta-diversity. A significant correlation between Enterococcus (jejunum NM samples) and Lactobacillus (cecum GF samples) with body weight and weight gain was found (p < 0.05). Moreover, Escherichia-Shigella in the cecum of NM was significantly correlated with weight gain (p < 0.05). The most abundant genera identified in the jejunal and cecal contents of GF were generally beneficial microbiota. They may also play a role in reducing the pathogenic effects of Escherichia coli in these rabbits.
Net Energy Value of a Cassava Chip Ration for Lactation in Holstein–Friesian Crossbred Dairy Cattle Estimated by Indirect Calorimetry
Authors: Gunha Thidarat , Kongphitee Kanokwan , Binsulong Bhoowadol , Sommart Kritapon
Published: July 2023

The objectives of this research were to (1) determine the feed intake, digestibility, and energy utilization and (2) estimate the net energy value of cassava chips consumed by lactating dairy cows. Four multiparous Holstein–Friesian crossbred cows at 139 ± 33 (mean ± SD) day in milk were assigned according to a 4 × 4 Latin square design with four periods. The four treatments included a diet substituted with cassava chips on a 0%, 12%, 24%, and 36% dry matter (DM) basis in the basal diet. Indirect calorimetry with a head cage respiration system was used to determine nutrient and energy utilization. Increasing the number of cassava chips in the diet resulted in a linear increase (p < 0.05) in nutrient intake and digestibility but a linear decrease (p < 0.01) in crude protein (CP) and fiber. The enteric methane yield and intensity were not affected (p > 0.05), while energy was lost as feces and urine reduced linearly (p < 0.05). Milk yield and milk composition (protein, fat, lactose) also increased linearly (p < 0.05). The net energy requirement for the maintenance of the lactating cows was estimated as 327 kJ/kg of metabolic body weight, and the efficiency of metabolizable energy used for lactation was 0.66. The estimated net energy value of cassava chips for lactation was 8.03 MJ/kg DM.
Air Quality Assessment in Pig Farming: The Italian Classyfarm
Authors: Buoio Eleonora , Cialini Chiara , Costa Annamaria
Published: July 2023

On 24 September 2019, the Ministry of Health issued an explanatory circular containing clarifications on the implementation methods of the National Improvement Plan for the application of Legislative Decree 122/2011. The Plan states that “In all farms where weaning or fattening pigs are raised and in breeding farms which wean piglets (excluding those for self-consumption), a risk assessment is carried out by the veterinarian on the basis of three levels: insufficient, room for improvement and optimal”. ClassyFarm, a risk assessment tool for livestock farming, is applied in Italy to evaluate the level of welfare and management of animals from a variety of points of view. Essentially, the categorization risk introduced by ClassyFarm in pig farming depended on the obligation stated by the EU in Decree 122/2011 to avoid tail docking in piglets and, at the same time, to reduce the stressor aspects able to induce aggressive behavior among pigs, improving the welfare and health status of animals. Since ClassyFarm evaluates many aspects of the management of animal farming, our aims in this review are to discuss the topic from an environmental point of view: (1) to frame the indications of ClassyFarm to make a farm risk assessment based on pigs’ welfare; (2) to review environmental quality assessment in pig farms, and its repercussions on animal health and welfare; (3) to describe the most used sampling techniques of air pollutants measurements.
An Overview on Assay Methods to Quantify ROS and Enzymatic Antioxidants in Erythrocytes and Spermatozoa of Small Domestic Ruminants
Authors: Pasciu Valeria , Nieddu Maria , Sotgiu Francesca Daniela , Baralla Elena , Berlinguer Fiammetta
Published: July 2023

The present review aims to provide an overview of the assay methods for the quantification of ROS and principal enzymatic antioxidants as biomarkers of oxidative stress in erythrocytes and spermatozoa of small domestic ruminants. A complete literature search was carried out in PubMed, Scopus and the World Wide Web using relevant keywords and focusing on the last five years (2018–2023). Among spectrophotometry, fluorometry and chemiluminescence, the most widely used method for ROS assay is fluorometry, probably because it allows to simultaneously assay several ROS, using different probes, with greater economic advantages. Regarding intracellular antioxidant enzymes, recent literature reports only spectrophotometric methods, many of which use commercial kits. The use of a less sensitive but cheapest method is suitable because both erythrocytes and spermatozoa samples are highly concentrated in domestic ruminant species. All methods considered in this review have been found to be appropriate; in general, the differences are related to their costs and sensitivity. Quantification of ROS and enzymatic antioxidant activity in erythrocytes and spermatozoa may find application in the study of the welfare and health status of small domestic ruminants for monitoring livestock production.
Measurement of Ammonia and Hydrogen Sulfide Emission from Three Typical Dairy Barns and Estimation of Total Ammonia Emission for the Chinese Dairy Industry
Authors: Shi Zhifang , Xi Lei , Zhao Xin
Published: July 2023

There is an urgent need for accurate measurement for emissions of ammonia (NH3) and hydrogen sulfide (H2S) in dairy barns in order to obtain reliable emission inventories and to develop and evaluate abatement strategies. This experiment was performed on three dairy farms in central China during 14 consecutive days in the winter 2020. Concentrations of NH3 and H2S were measured every two hours. The samples were taken inside and outside of barns from 7 sites at two heights (at floor and 1.5 over the floor). The results show that the average NH3 concentration was 2.47 mg/m3 with a maximum of 4.62 mg/m3, while the average H2S concentration was 0.179 mg/m3 with a maximum of 0.246 mg/m3. Lactating cows produced significantly more NH3 (3.73 mg/m3 versus 2.34 mg/m3) and H2S (0.24 mg/m3 versus 0.14 mg/m3) than non-lactating cows. NH3 and H2S concentrations were higher at 0 m than at 1.5 m, especially during the day. In addition, the average daily emission rates per animal unit (AU = 500 kg weight) were 23.5 g and 0.21 g for NH3 and H2S, respectively. The emission rate for NH3 was then used to extrapolate the NH3 emission from the Chinese dairy production. Our estimation for 2016 was 0.45 Tg, and it could reach 1.35 Tg by 2050. These numbers reflected our first attempt to calculate emission inventories for the Chinese dairy industry. Our results also suggest that more concrete measures must be taken to reduce the uncertainties of NH3 emissions from dairy cow production in China.
Diagnostic Performance of Multi-Detector Computed Tomography Arthrography and 3-Tesla Magnetic Resonance Imaging to Diagnose Experimentally Created Articular Cartilage Lesions in Equine Cadaver Stifles
Authors: Bolz Nico M. , Sánchez-Andrade José Suárez , Torgerson Paul R. , Bischofberger Andrea S.
Published: July 2023

Background: The purpose of the study was to determine the diagnostic performance of computed tomographic arthrography (CTA) and 3-Tesla magnetic resonance imaging (MRI) for detecting artificial cartilage lesions in equine femorotibial and femoropatellar joints. Methods: A total of 79 cartilage defects were created arthroscopically in 15 cadaver stifles from adult horses in eight different locations. In addition, 68 sites served as negative controls. MRI and CTA (80–160 mL iodinated contrast media at 87.5 mg/mL per joint) studies were obtained and evaluated by a radiologist unaware of the lesion distribution. The stifles were macroscopically evaluated, and lesion surface area, depth, and volume were determined. The sensitivity and specificity of MRI and CTA were calculated and compared between modalities. Results: The sensitivity values of CTA (53%) and MRI (66%) were not significantly different (p = 0.09). However, the specificity of CTA (66%) was significantly greater compared to MRI (52%) (p = 0.04). The mean lesion surface area was 11 mm2 (range: 2–54 mm2). Greater lesion surface area resulted in greater odds of lesion detection with CTA but not with MRI. Conclusions: CTA achieved a similar diagnostic performance compared to high-field MRI in detecting small experimental cartilage lesions. Despite this, CTA showed a higher specificity than MRI, thus making CTA more accurate in diagnosing normal cartilage. Small lesion size was a discriminating factor for lesion detection. In a clinical setting, CTA may be preferred over MRI due to higher availability and easier image acquisition.
The Role of Sound in Livestock Farming—Selected Aspects
Authors: Olczak Katarzyna , Penar Weronika , Nowicki Jacek , Magiera Angelika , Klocek Czesław
Published: July 2023

To ensure the optimal living conditions of farm animals, it is essential to understand how their senses work and the way in which they perceive their environment. Most animals have a different hearing range compared to humans; thus, some aversive sounds may go unnoticed by caretakers. The auditory pathways may act through the nervous system on the cardiovascular, gastrointestinal, endocrine, and immune systems. Therefore, noise may lead to behavioral activation (arousal), pain, and sleep disorders. Sounds on farms may be produced by machines, humans, or animals themselves. It is worth noting that vocalization may be very informative to the breeder as it is an expression of an emotional state. This information can be highly beneficial in maintaining a high level of livestock welfare. Moreover, understanding learning theory, conditioning, and the potential benefits of certain sounds can guide the deliberate use of techniques in farm management to reduce the aversiveness of certain events.
Suppression of MUC1-Overexpressing Tumors by a Novel MUC1/CD3 Bispecific Antibody
Authors: Fang Jun , Lai Shifa , Yu Haoyang , Ma Lan
Published: July 2023

Mucin1 (MUC1) is abnormally glycosylated and overexpressed in a variety of epithelial cancers and plays a critical role in tumor progression. MUC1 has received remark attention as an oncogenic molecule and is considered a valuable tumor target for immunotherapy, while many monoclonal antibodies (mAbs) targeting MUC1-positive cancers in clinical studies lack satisfactory results. It would be highly desirable to develop an effective therapy against MUC1-expressing cancers. In this study, we constructed a novel T cell-engaging bispecific antibody (BsAb) targeting MUC1 and CD3 with the Fab-ScFv-IgG format. A high quality of MUC1-CD3 BsAb can be acquired through a standard method. Our study suggested that this BsAb could specifically bind to MUC1- and CD3-positive cells and efficiently enhance T cell activation, cytokine release, and cytotoxicity. Furthermore, our study demonstrated that this BsAb could potently redirect T cells to eliminate MUC1-expressing tumor cells in vitro and significantly suppress MUC1-positive tumor growth in a xenograft mouse model. Thus, T cell-engaging MUC1/CD3 BsAb could be an effective therapeutic approach to combat MUC1-positive tumors and our MUC1/CD3 BsAb could be a promising candidate in clinical applications for the treatment of MUC1-positive cancer patients.
A Study Review of the Appropriateness of Oral Antibiotic Discharge Prescriptions in the Emergency Department at a Rural Hospital in Mississippi, USA
Authors: Le Giang , Ivy Madalyn , Dickey Sharon , Welch Ron , Stallings Danielle
Published: July 2023

Antimicrobial therapy in emergency departments (EDs) is usually empiric in nature. Due to workload and a goal to reduce patient wait times, providers often make rapid decisions regarding antibiotic prescriptions for discharge. A review of current empiric prescribing practices would determine the appropriateness of oral antibiotic discharge prescriptions from EDs. A single-center retrospective electronic health record review of all adult patients with an ED visit from 1 June 2019, to 30 June 2021 who received at least one oral antibiotic prescription at discharge from Baptist Memorial Hospital-Golden Triangle was conducted. The primary outcome was the assessment of appropriate antibiotic discharge prescriptions. The parameters for appropriateness included empiric drug selection, dosage, frequency, duration, and subsequent cultures and sensitivities. Of the 18,289 identified records, 421 patients were randomly sampled with 400 patients included in the final analysis. Of these, 190 (47.8%) discharge oral antibiotic prescriptions were assessed as appropriate and 209 (52.3%) discharge oral antibiotic prescriptions were assessed as inappropriate based on the guideline recommendations. With approximately half of the patients receiving discharge antibiotics that did not fully follow the guideline recommendations, there is a need for provider education, pharmacist intervention, and antimicrobial stewardship programs focusing on this practice.
Convolutional Neural Network for Segmenting Micro-X-ray Computed Tomography Images of Wood Cellular Structures
Authors: Arzola-Villegas Xavier , Báez Carlos , Lakes Roderic , Stone Donald S. , O’Dell Jane , Shevchenko Pavel , Xiao Xianghui , De Carlo Francesco , Jakes Joseph E.
Published: July 2023

To further enhance the performance of wood products, improved tools are needed to study in situ cellular scale phenomena like mechanical deformations and moisture swelling. Micro-X-ray computed tomography (μXCT) using brilliant synchrotron light sources now has the spatial and temporal resolution for real-time visualization of phenomena in three-dimensional cellular structures. However, the tradeoff for speed includes the loss of intensity contrast between different types of materials within the imaged structure, such as cell wall and air in wood. This loss of contrast prevents traditional histogram-based segmentation methods from being used effectively. A new convolutional neural network (CNN) approach was therefore developed to segment fast μXCT images of wood into cell wall and air volumes. The fast μXCT and segmentation were demonstrated in the study of moisture swelling in loblolly pine (Pinus taeda) earlywood and latewood cellular structures conditioned at 0%, 33%, 75%, and 95% relative humidity (RH). The CNN segmentation results had a mean intersection over union (IoU) metric accuracy of 96%. Initial analysis of the swelling in the latewood revealed cell walls swelled about 25% when conditioned from 0% to 95% RH. Additionally, the widths of ray cell lumina in the transverse plane of latewood could be observed to increase at higher RH. The segmentation method presented here will facilitate future quantitative analyses in in situ μXCT studies of wood and other similar cellular materials.
Forecast Analysis of Pollutant Emissions of Cruise Ship Routes in Western Mediterranean
Authors: Esteve-Pérez Jerónimo , Río-González Manuel del , Gutiérrez-Romero José Enrique
Published: July 2023

The cruise shipping industry has a steadfast commitment to the environmentally sustainable operation of ships through many initiatives to reduce greenhouse gas emissions. This paper presents a forecast analysis of the emission inventory of NOx, SO2, and CO2 during the sailing phase associated with cruise routes in the Western Mediterranean. Five scenarios were raised to obtain projections about future emissions considering the evolution of cruise traffic; the implementation of the Sulfur Emission Control Area (SECA) in the Mediterranean by 1 May 2025, and variations in the specific fuel consumption of the engines of the cruise ships. To this extent, a time series of cruise calls during 2019 in Mediterranean ports is used to identify the main routes and their associated emission inventory. Network analysis is applied to identify the routes associated with the cruise calls, and the emission inventory is estimated using a bottom-up approach. A total of 292 cruise routes were identified. The estimations reveal that the emissions of the cruise activities assessed are approximately equivalent to 0.58% of NOx, 0.28% of SO2, and 1% of CO2 of the entirety of the emissions associated with maritime transport at the European level in 2019. The projection of the scenario with the highest cruise activity and the SECA being enforced leads to a reduction of 77% of the emissions of SO2 considering the activity of 2019. The research conducted enables us to assess the environmental impact of the intense cruise ship activity registered in the Mediterranean and the positive footprint of the implementation of new regulations on emissions.
Study of Temperature Effect on Cascade Characteristics of Nanofluidic Energy Absorption System
Authors: Zhang Yafei , Wang Haitao , Xiao Hongjiu , Liu Shenlei , Wei Wenlan , Dou Yihua
Published: July 2023

Nanofluidic energy absorption system (NEAS) with cascade energy absorption characteristics can absorb energy on different levels simultaneously in one system, which greatly enriches its functions and applications. The pore structure and size distribution of porous media play a crucial role in the design and construction of cascade nanofluidic systems. In this paper, two cascade pore models were constructed using carbon nanotubes with different diameters, one was the model of two tubes with both one end immersed in water (DNEAS), and the other was the model of two tubes end to end, with the end of the big tube immersed in water (SNEAS). The effects of temperature-coupled pore size on the infiltration processes of water molecules into two models were investigated. The fitting correlations between critical pore size difference and temperature were established. The microscopic mechanism of temperature effect was illuminated. With the increase in temperature, systems displaying cascade characteristics transformed into a single-stage system without cascade characteristics. Due to the significant size effect of system temperature, the critical pore difference increased with both system temperature and the pore size. The research results expanded the basic database of cascade nanofluidic systems and provided guidance for the application design of cascade nanofluidic systems.
Kernel Regression Residual Decomposition-Based Polynomial Frequency Modulation Integral Algorithm to Identify Physical Parameters of Time-Varying Systems under Random Excitation
Authors: Liu Hui , Shi Zhiyu
Published: July 2023

The physical parameters (stiffness, damping) of time-varying (TV) systems under random excitation provide valuable information for their working condition but they are often overwhelmed by noise interference. To overcome this problem, this paper presents a novel multi-level kernel regression residual decomposition method, which can not only effectively separate each modal component from the raw vibration acceleration signal, but also eliminate noise interference. Additionally, the multiple degree-of-freedom (DOF) parameter identification problem is transformed into a single DOF parameter identification problem. Combined with the derived polynomial frequency modulation integral algorithm and the cross-correlation theory based on the fractional Fourier ambiguity function, a physical parameter identification method is proposed. The method provides a new idea in modeling TV systems and identifying physical parameters under random excitation. To demonstrate the effectiveness of the proposed method, numerical simulations are conducted with three different cases of variation (variation, quadratic variation, and periodic variation) in time. Moreover, its robustness is evaluated by adding different signal-to-noise ratio levels of noise (20 dB, 50 dB, 100 dB) to the input vibration acceleration signal. The analysis results confirm the performance of the proposed method for the parameter identification of TV systems under random excitation.
Evaluating the Influence of Sand Particle Morphology on Shear Strength: A Comparison of Experimental and Machine Learning Approaches
Authors: Daghistani Firas , Abuel-Naga Hossam
Published: July 2023

Particulate materials, such as sandy soil, are everywhere in nature and form the basis for many engineering applications. The aim of this research is to investigate the particle shape, size, and gradation of sandy soil and how they relate to shear strength, which is an essential characteristic that impacts soil stability and mechanical behaviour. This will be achieved by employing a combination of experimental methodology, which includes the use of a microscope direct shear apparatus, and machine learning techniques, namely multiple linear regression and random forest regression. The experimental findings reveal that angular-shaped sand particles enhance the shear strength characteristics compared to spherical, rounded ones. Similarly, coarser sand particles improve these characteristics compared to finer sand particles, as do well-graded particles when compared to poorly graded ones. The machine learning findings show the validity of both models in predicting shear strength when compared to the experimental results, showing high accuracy. The models are designed to predict shear strength of sand considering six input features: mean particle size, uniformity coefficient, curvature coefficient, dry density, normal stress, and particle regularity. The most important features from both models were identified. In addition, an empirical equation for calculating shear strength was developed through multiple linear regression analysis using the six features.
Sparse Subgraph Prediction Based on Adaptive Attention
Authors: Li Weijun , Gao Yuxiao , Li Ang , Zhang Xinyong , Gu Jianlai , Liu Jintong
Published: July 2023

Link prediction is a crucial problem in the analysis of graph-structured data, and graph neural networks (GNNs) have proven to be effective in addressing this problem. However, the computational and temporal costs associated with large-scale graphs remain a concern. This study introduces a novel method for link prediction called Sparse Subgraph Prediction Based on Adaptive Attention (SSP-AA). The method generates sparse subgraphs and utilizes Graph SAmple and aggreGatE (GraphSAGE) for prediction, aiming to reduce computation and time costs while providing a foundation for future exploration of large-scale graphs. Certain key issues in GraphSAGE are addressed by integrating an adaptive attention mechanism and a jumping knowledge module into the model. To address the issue of adaptive weight distribution in GraphSAGE, an aggregation function is employed, which is based on the attention mechanism. This modification enables the model to distribute weights adaptively among neighboring nodes, significantly improving its ability to capture node relationships. Furthermore, to tackle the common issue of over-smoothing in GNNs, a jumping knowledge module is integrated, enabling information sharing across different layers and providing the model with the flexibility to select the appropriate representation depth based on the specific situation. By enhancing the quality of node representations, SSP-AA further boosts the performance of GraphSAGE in various prediction tasks involving graph-structured data.
Boiling vs. Microwave Heating—The Impact on Physicochemical Characteristics of Bell Pepper (Capsicum annuum L.) at Different Ripening Stages
Authors: Olędzki Remigiusz , Harasym Joanna
Published: July 2023

Background: The present study addresses this research gap by evaluating the impact of boiling in water and microwaving on the bioactivity characteristics of bell peppers at different ripening stages. Methods: The total polyphenols, DPPH, ABTS and FRAP were used for the evaluation of the antioxidant potential qualitatively and quantitatively, and the simple reductive sugar texture and color changes were measured. Results: Microwave heating appears to be a favorable treatment in the case of preservation of most of the antioxidant potential. Green and red bell peppers were more resistant to the treatments, while the yellow stage was the one in which the changes were observed the most. Conclusions: However, the results indicate that from a consumer standpoint, microwave heating treatment is more beneficial for red peppers. In contrast, hot water cooking is more beneficial for green and yellow peppers.
Application-Specific Integrated Circuit of an Inter-IC Sound Digital Filter for Audio Systems
Authors: Davila-Velarde Rene , Ramos-Contreras Ricardo , Pizano-Escalante Luis , Longoria-Gandara Omar , Aguilera-Galicia Cuauhtémoc
Published: July 2023

In digital audio systems, filters and equalizers are essential modules for audio improvement at the input and output stages. Due to their computational complexity, most audio tasks are processed with digital signal processors. Due to the fact that latency in audio systems is a critical specification and audio trends require higher sample rates, noise canceling, and bigger data sizes, having an independent high-resolution equalizer would reduce the computational power needed for audio systems. This research had the goal of designing and implementing a hardware architecture for a configurable filter bank based on finite impulse response (FIR) filters and a noise-cancellation stage with an inter-integrated circuit (I2C) communication interface, which allows the filter configuration. The system was implemented as a standalone integrated circuit (IC) for which its inputs were the inter-IC sound (I2S) bus control signals. The digital audio system was optimized to perform one-cycle convolutional operations by implementing a vector–vector arithmetic logic unit. Furthermore, this applied research provides the register transfer level description and the functional verification of the digital design, the system-on-chip (SoC) implementation in TSMC 180 nm technology, and the post-silicon validation with a printed circuit board for testing the output digital signals of the system.
Interferences of Electromagnetic Pulses on Microcontroller Units
Authors: Fan Linjing , Zu Xudong , Huang Zhengxiang
Published: July 2023

In this study, electromagnetic interference testing of microcontroller units (MCUs) under different electromagnetic pulse (EMP) amplitudes, full width at half maximum (FWHM), and at different angles was carried out on an EMP cell. The coupling path of the radiation-type EMP experiment on the circuit board is random. However, in several experiments with two pins specific to a certain integrated circuit, by measuring the interference voltage of MCU pins, the statistical results indicate that as the pressure of the air gap switch of the power source increased, both the breakdown voltage and the electric field in the transverse electromagnetic (TEM) cell increased, resulting in higher electromagnetic interference (EMI) received by these two pins. As the capacitance of the storage capacitor increased, the EMI also increased. In addition, the results showed that the interference of EMP on the MCU had strong directionality; i.e., path selectivity, which was related to the structure of the MCU. X-ray imaging of the destroyed MCU showed that when the internal wiring direction of the pin is consistent with the propagation direction of the interference pulse, the EMI was minimal or even unnoticeable.
Effect of Superhydrophobic Nano-SiO2 on the Hydraulic Conductivity of Expansive Soil and Analysis of Its Mechanism
Authors: Luo Xiaoqian , Kong Lingwei , Bai Wei
Published: July 2023

The present work determined the influence of superhydrophobic nano-SiO2 on the hydraulic conductivity and pore size distribution of expansive soil, and analysed the mechanism of modification between superhydrophobic nano-SiO2 and expansive soil from a microscopic view. Superhydrophobic nano-SiO2 was added to expansive soil as a modifier. Our samples were of two types, i.e., unmodified (without nano-SiO2) and modified (with 0.2%, 0.4%, 0.6%, 0.8%, and 1.0% nano-SiO2 by weight of the parent soil). The hydraulic conductivity decreased with increasing nano-SiO2 content. Fourier transform mid-infrared test revealed that some silanols in soil and nano-SiO2 were dehydrated and condensed to form siloxanes. We inferred that nano-SiO2 can attach onto the surface of soil particles to form a hydrophobic membrane, which reduced the soil expansion and the change in pore size distribution. And microscopic tests showed that the pore volume and hydrophilicity of the soil samples decreased with increasing SiO2 content. According to the Young–Laplace equation, the minimum permeable pore radius was calculated in the hydraulic-conductivity test. With increasing nano-SiO2 content, the volume of permeable pore decreased. It had an excellent linear relationship with the hydraulic conductivity and permeable pore volume of samples containing different nano-SiO2 contents. Therefore, superhydrophobic nano-SiO2 could effectively reduce hydraulic conductivity by changing the pore size distribution of expansive soil.
Computational Flow Dynamic Analysis in Left Atrial Appendage Thrombus Formation Risk: A Review
Authors: Valvez Sara , Oliveira-Santos Manuel , Piedade Ana P. , Gonçalves Lino , Amaro Ana M.
Published: July 2023

Atrial fibrillation (AF) is a common cardiac arrhythmia characterized by irregular and rapid electrical activity in the atria, leading to ineffective contraction and poor blood flow. More than 90% of the left atrial (LA) thrombi that cause thromboembolic events during atrial fibrillation (AF) develop in the left atrial appendage (LAA). AF modifies the hemodynamics of the left atrium, which can result in thrombosis of the LAA, systemic embolism, and stroke. The current options to reduce thromboembolic events are oral anticoagulation, surgical LAA exclusion, or percutaneous LAA occlusion. However, the mechanism underlying thrombus development in the LAA remains poorly understood. Computational fluid dynamics (CFD) analysis can be used to better understand the risk of thrombus formation and subsequent embolic events. CFD enables the simulation and visualization of blood flow patterns within the heart, including complex structures such as the LAA. Using CFD, researchers can analyze the hemodynamics of blood flow, identify areas of stagnation or turbulence, and predict the risk of thrombus formation. The correlation between blood flow dynamics, atrial fibrillation, and the risk of stroke has been highlighted by CFD studies investigating the underlying mechanism of thrombus formation in the LAA. This review study intends to provide a comprehensive overview of the factors involved in thrombus formation and their implications for clinical practice by synthesizing the insights acquired from these CFD studies.
Wave Climate Variability along the Coastlines of Senegal over the Last Four Decades
Authors: Samou Marcellin Seujip , Bertin Xavier , Sakho Issa , Lazar Alban , Sadio Mamadou , Diouf Mouhamadou Bachir
Published: July 2023

Knowledge of wave climate is essential for efficient management of the world’s coastal areas. Senegal is a relevant case, given its high coastal vulnerability to energetic wave conditions. This study investigates wave climates along the coastal zone of Senegal based on a new high-resolution hindcast covering the period 1980–2021. This study evaluates the average, seasonal, and extreme values for the significant wave heights (Hs), periods (Tm02/Tp), and mean directions (DIR). In boreal winter, the wave climate is dominated by swells coming from the North-Atlantic lows. In contrast, in boreal summer, the Southern Coast (from Dakar to Casamance) is exposed to swells generated in the South Atlantic Ocean. Throughout their refraction around the Dakar Peninsula, NW swells rotate by ~100° from NW to SW, while their Hs is roughly halved when reaching the Southern Coast of Senegal. Over the studied period, trends in Hs are weak (~0.6 cm.decade−1) on the Northern Coast and double on the Southern Coast (~1.2 cm.decade−1), mostly due to an increase during boreal summer (2 cm.decade−1). The wave periods show weak trends (~0.05 s.decade−1), and DIRs show weak counterclockwise rotation (−1°.decade−1). These trends are explained by the main climate modes of the Atlantic Ocean (NAO/EA during winter, SAM during summer) and are important for future research and long-term monitoring of the Senegalese Coast.
Unveiling the Potential Distribution of the Highly Threatened Madeira Pipistrelle (Pipistrellus maderensis): Do Different Evolutionary Significant Units Exist?
Authors: Teixeira Sérgio , Smeraldo Sonia , Russo Danilo
Published: July 2023

The isolation of islands has played a significant role in shaping the unique evolutionary histories of many species of flora and fauna, including bats. One notable example is the Madeira pipistrelle (Pipistrellus maderensis), which inhabits the Macaronesian archipelagos of the Azores, Madeira, and the Canary Islands. Despite the high biogeographic and conservation importance of this species, there is limited information on its ecology and evolutionary history across different archipelagos. In our study, we employed species distribution models (SDMs) to identify suitable habitats for the Madeira pipistrelle and determine the environmental factors influencing its distribution. Additionally, we conducted molecular comparisons using mitochondrial DNA data from various Macaronesian islands. Molecular analyses provided compelling evidence for the presence of distinct Evolutionary Significant Units on the different archipelagos. We identified distinct haplotypes in the populations of Madeira and the Canary Islands, with a genetic distance ranging from a minimum of 2.4% to a maximum of 3.3% between samples from different archipelagos. In support of this, SDMs highlighted relevant dissimilarities between the environmental requirements of the populations of the three archipelagos, particularly the climatic niche. Our research demonstrates that deeper investigations that combine ecological, morphological, and genetic areas are necessary to implement tailored conservation strategies.
Dissecting the Nuclear Import of the Ribosomal Protein Rps2 (uS5)
Authors: Steiner Andreas , Favre Sébastien , Mack Maximilian , Hausharter Annika , Pillet Benjamin , Hafner Jutta , Mitterer Valentin , Kressler Dieter , Pertschy Brigitte , Zierler Ingrid
Published: July 2023

The ribosome is assembled in a complex process mainly taking place in the nucleus. Consequently, newly synthesized ribosomal proteins have to travel from the cytoplasm into the nucleus, where they are incorporated into nascent ribosomal subunits. In this study, we set out to investigate the mechanism mediating nuclear import of the small subunit ribosomal protein Rps2. We demonstrate that an internal region in Rps2, ranging from amino acids 76 to 145, is sufficient to target a 3xyEGFP reporter to the nucleus. The importin-β Pse1 interacts with this Rps2 region and is involved in its import, with Rps2 residues arginine 95, arginine 97, and lysine 99 being important determinants for both Pse1 binding and nuclear localization. Moreover, our data reveal a second import mechanism involving the N-terminal region of Rps2, which depends on the presence of basic residues within amino acids 10 to 28. This Rps2 segment overlaps with the binding site of the dedicated chaperone Tsr4; however, the nuclear import of Rps2 via the internal as well as the N-terminal nuclear-targeting element does not depend on Tsr4. Taken together, our study has unveiled hitherto undescribed nuclear import signals, showcasing the versatility of the mechanisms coordinating the nuclear import of ribosomal proteins.
Inhibition of Cell Proliferation and Cell Death by Apigetrin through Death Receptor-Mediated Pathway in Hepatocellular Cancer Cells
Authors: Bhosale Pritam Bhagwan , Kim Hun Hwan , Abusaliya Abuyaseer , Jeong Se Hyo , Park Min Yeong , Kim Hyun-Wook , Seong Je Kyung , Ahn Meejung , Park Kwang Il , Heo Jeong Doo , Kim Young Sil , Kim Gon Sup
Published: July 2023

Epidemiologic research recommends using flavonoids in the diet due to their overall health benefits. Apigetrin (Apigenin 7-O-glucoside) is a glycoside phytonutrient found in fruits and vegetables and known for different biological activities such as antioxidant and anti-inflammatory properties. Hepatocellular cancer (HCC) is a major health concern because of its adverse prognosis and side effects of chemotherapeutic agents. In the present study, we determine the impact of apigetrin on HepG2 cells and its cell death mechanism. Apigetrin reduced HepG2 cell proliferation with morphological changes and floating cells in treated cells. Colony formation and wound healing assays showed a reduced cell number in treatment groups. Further, we checked for the cell cycle through flow cytometry to understand the cell death mechanism. Apigetrin induced G2/M phase arrest in HepG2 cells by regulating Cyclin B1 and CDK1 protein levels in HepG2 cells. Annexin V and propidium iodide (PI) staining was performed to confirm the apoptotic cell population in treated groups. At the higher concentration, apigetrin showed a late apoptotic population in HepG2 cells. Chromatin condensation was also found in the treatment groups. Western blot analysis showed an increased expression of extrinsic apoptotic proteins such as FasL, Cleaved caspase 8, Cleaved caspase 3, and cleavage of PARP. In comparison, intrinsic apoptotic pathway markers showed no changes in Bax, Bcl-xL, and Cleaved caspase 9. Altogether, these findings strongly indicate that apigetrin causes cell death in HepG2 cells through the extrinsic apoptotic pathway, and that the intrinsic/mitochondrial pathway is not involved.
Characterizing the Impedance Properties of Dry E-Textile Electrodes Based on Contact Force and Perspiration
Authors: Ravichandran Vignesh , Ciesielska-Wrobel Izabela , Rumon Md Abdullah al , Solanki Dhaval , Mankodiya Kunal
Published: July 2023

Biopotential electrodes play an integral role within smart wearables and clothing in capturing vital signals like electrocardiogram (ECG), electromyogram (EMG), and electroencephalogram (EEG). This study focuses on dry e-textile electrodes (E1–E6) and a laser-cut knit electrode (E7), to assess their impedance characteristics under varying contact forces and moisture conditions. Synthetic perspiration was applied using a moisture management tester and impedance was measured before and after exposure, followed by a 24 h controlled drying period. Concurrently, the signal-to-noise ratio (SNR) of the dry electrode was evaluated during ECG data collection on a healthy participant. Our findings revealed that, prior to moisture exposure, the impedance of electrodes E7, E5, and E2 was below 200 ohm, dropping to below 120 ohm post-exposure. Embroidered electrodes E6 and E4 exhibited an over 25% decrease in mean impedance after moisture exposure, indicating the impact of stitch design and moisture on impedance. Following the controlled drying, certain electrodes (E1, E2, E3, and E4) experienced an over 30% increase in mean impedance. Overall, knit electrode E7, and embroidered electrodes E2 and E6, demonstrated superior performance in terms of impedance, moisture retention, and ECG signal quality, revealing promising avenues for future biopotential electrode designs.
Advances in Simple, Rapid, and Contamination-Free Instantaneous Nucleic Acid Devices for Pathogen Detection
Authors: Wang Yue , Wang Chengming , Zhou Zepeng , Si Jiajia , Li Song , Zeng Yezhan , Deng Yan , Chen Zhu
Published: July 2023

Pathogenic pathogens invade the human body through various pathways, causing damage to host cells, tissues, and their functions, ultimately leading to the development of diseases and posing a threat to human health. The rapid and accurate detection of pathogenic pathogens in humans is crucial and pressing. Nucleic acid detection offers advantages such as higher sensitivity, accuracy, and specificity compared to antibody and antigen detection methods. However, conventional nucleic acid testing is time-consuming, labor-intensive, and requires sophisticated equipment and specialized medical personnel. Therefore, this review focuses on advanced nucleic acid testing systems that aim to address the issues of testing time, portability, degree of automation, and cross-contamination. These systems include extraction-free rapid nucleic acid testing, fully automated extraction, amplification, and detection, as well as fully enclosed testing and commercial nucleic acid testing equipment. Additionally, the biochemical methods used for extraction, amplification, and detection in nucleic acid testing are briefly described. We hope that this review will inspire further research and the development of more suitable extraction-free reagents and fully automated testing devices for rapid, point-of-care diagnostics.
Hemagglutination Assay via Optical Density Characterization in 3D Microtrap Chips
Authors: Nam Sung-Wook , Jeon Dong-Gyu , Yoon Young-Ran , Lee Gang Ho , Chang Yongmin , Won Dong Il
Published: July 2023

Hemagglutination assay has been used for blood typing and detecting viruses, thus applicable for the diagnosis of infectious diseases, including COVID-19. Therefore, the development of microfluidic devices for fast detection of hemagglutination is on-demand for point-of-care diagnosis. Here, we present a way to detect hemagglutination in 3D microfluidic devices via optical absorbance (optical density, OD) characterization. 3D printing is a powerful way to build microfluidic structures for diagnostic devices. However, mixing liquid in microfluidic chips is difficult due to laminar flow, which hampers practical applications such as antigen-antibody mixing. To overcome the issue, we fabricated 3D microfluidic chips with embedded microchannel and microwell structures to induce hemagglutination between red blood cells (RBCs) and antibodies. We named it a 3D microtrap chip. We also established an automated measurement system which is an integral part of diagnostic devices. To do this, we developed a novel way to identify RBC agglutination and non-agglutination via the OD difference. By adapting a 3D-printed aperture to the microtrap chip, we obtained a pure absorbance signal from the microchannels by eliminating the background brightness of the microtrap chip. By investigating the underlying optical physics, we provide a 3D device platform for detecting hemagglutination.