bluuebunny/crossref_metadata_2025 huggingface dataset:
Presentation and management of lateral sinus thrombosis following posterior fossa surgery
Authors: Apra Caroline , Kotbi Owais , Turc Guillaume , Corns Robert , Pagès Mélanie , Souillard-Scémama Raphaëlle , Dezamis Edouard , Parraga Eduardo , Meder Jean-François , Sauvageon Xavier , Devaux Bertrand , Oppenheim Catherine , Pallud Johan
Published: February 2016

OBJECTIVE

There are no guidelines for the management of postoperative lateral sinus thrombosis following posterior fossa surgery. Introducing treatment-dose anticoagulant therapy during the immediate postoperative period increases the risk of intracranial bleeding. This study assessed the incidence of and risk factors associated with postoperative lateral sinus thrombosis and the complications related to thrombosis and/or anticoagulation.

METHODS

This study was a retrospective monocentric analysis of adult patients who underwent surgical removal of a posterior fossa space-occupying lesion with available postoperative imaging. Postoperative lateral sinus thrombosis was defined as a T2* hypointensity within the venous sinus and/or a filling defect on postcontrast MRI or CT scan.

RESULTS

Among 180 patients, 12 (6.7%; 95% CI 3.0–10.4) were found to have lateral sinus thrombosis on postoperative imaging, none of whom were symptomatic. Unadjusted risk factors for postoperative lateral sinus thrombosis were a history of deep venous thrombosis (p = 0.016), oral contraceptive pill (p = 0.004), midline surgical approach (p = 0.035), and surgical exposure of the sinus (p < 0.001). Seven of the patients (58.3%) with a postoperative lateral sinus thrombosis received immediate treatment-dose anticoagulant therapy. Lateral sinus recanalization occurred radiologically at a mean time of 272 ± 23 days in 85.7% of patients (6 of 7) undergoing treatment-dose anticoagulant therapy and in 20% of patients (1 of 5) not receiving treatment-dose anticoagulant therapy. Postoperative complications occurred in 56.2% of patients (9 of 16) who received treatment-dose curative anticoagulant therapy and in 27% of patients (45 of 164) who did not.

CONCLUSIONS

Incidental radiological lateral sinus thrombosis following posterior fossa surgery has an incidence of 6.7%. To further define the benefit-to-risk ratio of a treatment-dose anticoagulant therapy, a prospective trial should be considered.
Intraoperative neurophysiological monitoring during resection of infratentorial lesions: the surgeon's view
Authors: Slotty Philipp J. , Abdulazim Amr , Kodama Kunihiko , Javadi Mani , Hänggi Daniel , Seifert Volker , Szelényi Andrea
Published: February 2016

OBJECTIVE

Methods of choice for neurophysiological intraoperative monitoring (IOM) within the infratentorial compartment mostly include early brainstem auditory evoked potentials, free-running electromyography, and direct cranial nerve (CN) stimulation. Long-tract monitoring with somatosensory evoked potentials (SEPs) and motor evoked potentials (MEPs) is rarely used. This study investigated the incidence of IOM alterations during posterior fossa surgery stratified for lesion location.

METHODS

Standardized CN and SEP/MEP IOM was performed in 305 patients being treated for various posterior fossa pathologies. The IOM data were correlated with lesion locations and histopathological types as well as other possible confounding factors.

RESULTS

Alterations in IOM were observed in 158 of 305 cases (51.8%) (CN IOM alterations in 130 of 305 [42.6%], SEP/MEP IOM alterations in 43 of 305 [14.0%]). In 15 cases (4.9%), simultaneous changes in long tracts and CNs were observed. The IOM alterations were followed by neurological sequelae in 98 of 305 cases (32.1%); 62% of IOM alterations resulted in neurological deficits. Sensitivity and specificity for detection of CN deficits were 98% and 77%, respectively, and 95% and 85%, respectively, for long-tract deficits. Regarding location, brainstem and petroclival lesions were closely associated with concurrent CN IOM and SEP/MEP alterations.

CONCLUSIONS

The incidence of IOM alterations during surgery in the posterior fossa varied widely between different lesion locations and histopathological types. This analysis provides crucial information on the necessity of IOM in different surgical settings. Because MEP/SEP and CN IOM alterations were commonly observed during posterior fossa surgery, the authors recommend the simultaneous use of both modalities based on lesion location.
Postconcussion syndrome: demographics and predictors in 221 patients
Authors: Tator Charles H. , Davis Hannah S. , Dufort Paul A. , Tartaglia Maria Carmella , Davis Karen D. , Ebraheem Ahmed , Hiploylee Carmen
Published: February 2016

OBJECTIVE

The objective of this study was to determine the demographics and predictors of postconcussion syndrome (PCS) in a large series of patients using a novel definition of PCS.

METHODS

The authors conducted a retrospective cohort study of 284 consecutive concussed patients, 221 of whom had PCS on the basis of at least 3 symptoms persisting at least 1 month. This definition of PCS was uniformly employed and is unique in accepting an expanded list of symptoms, in shortening the postconcussion interval to 1 month from 3 months, and in excluding those with focal injuries such as hemorrhages and contusions.

RESULTS

The 221 cases showed considerable heterogeneity in clinical features of PCS. They averaged 3.3 concussions, with a range of 0 to 12 or more concussions, and 62.4% occurred during sports and recreation. The median duration of PCS was 7 months at the time of examination, with 11.8% lasting more than 2 years, and 23.1% with PCS had only 1 concussion. The average patient age was 27 years (range 10–74 years). The average number of persistent symptoms was 8.1; 26.2% had a previous psychiatric condition, attention-deficit disorder/attention-deficit hyperactivity disorder, a learning disability, or previous migraine headaches. The prevalence of arachnoid cysts and Chiari malformation in PCS exceeded the general population. Additionally, involvement in litigation, presence of extracranial injuries, amnesia and/or loss of consciousness, and female sex were predictive of reporting a high number of symptoms. A prior history of psychiatric conditions or migraines, cause of injury, number of previous concussions, and age did not significantly predict symptom number. Only the number of symptoms reported predicted the duration of PCS. To predict the number of symptoms for those who fulfilled PCS criteria according to the International Classification of Diseases, 10th Revision (ICD-10), and the Diagnostic and Statistical Manual of Mental Disorders, 4th Edition (DSM-IV), the number of previous concussions was significant.

CONCLUSIONS

PCS is commonly associated with multiple concussions, but 23.1% in the present series occurred after only 1 concussion. Most patients with PCS had multiple symptoms persisting for months or years. The median duration of PCS was 7 months, with a range up to 26 years. In only 11.3%, the PCS had ended at the time of consultation. Not all predictors commonly cited in the literature align with the findings in this study. This is likely due to differences in the definitions of PCS used in research. These results suggest that the use of ICD-10 and DSM-IV to diagnose PCS may be biased toward those who are vulnerable to concussions or with more severe forms of PCS. It is thus important to redefine PCS based on evidence-based medicine.
Hybridity: A Privilege of a Few or the Necessity for All in Amin Maalouf's In the Name of Identity
Authors: Alhathlool Khalid
Published: February 2016

This paper critically engages with Amin Maalouf's understanding of identity in his book In the Name of Identity (1997). Maalouf's intervention in the contentious discussion of identity consists of four principle points. Firstly, identity is composite. Secondly, identity is constructed through dialogic process. Thirdly, the understanding of identity today is predominately ‘tribal.’ Finally there is a need for the prevalence of the universal in the ‘era of globalization’. Maalouf's analysis rightly transcends the limitation of essentialist and singular conceptions of identity—in particular- cultural identity—in today's ‘postcolonial’’ world; however, his proposed alternative, and the assumptions upon which it rests, are equally problematic, especially for ‘postcolonial’ societies and immigrants/migrants in ‘Western’ countries. Maalouf's perspective on hybridity is riddled with contradictions: above all, the contradiction between understanding hybridity as a foundational position and as a deconstructive force of fixed identities and naturalised categories. This confusion arises when, on the one hand, cultures are understood as bounded and territorialised and, on the other, individuals are thought to belong simultaneously to these different, bounded cultures in full composite terms. In the first place there is an argument for sustaining purity, while the subsequent stage of identity formation advocates hybridity. Maalouf rejects purity as well as hybridity by appearing to sustain the two simultaneously. He cannot maintain this contradiction except through individualising the conception of identity. This paper argues that while Maalouf is able to problematise notions of ‘essentialist’ identities—what he dubs as ‘murderous identities’—and presents a moderately plausible case for ‘hybridity’, he fails to depart from a hegemonic and reified notion of a ‘universal’ or ‘cosmopolitan’ definition of identity which in effect operates as a code for Eurocentric ideas of identity and being. Thus, Maalouf's ‘speaking for’ postcolonial and migrant people/cultures and ‘speaking back’ against neo-conservative world-view is never quite able to escape the latter's ideological moorings.
Happiness from the Skies, or a New Death from Above? #cokedrones in the city
Authors: Overington Caitlin , Phan Thao
Published: February 2016

The production of the #cokedrone YouTube advertisement by Coca-Cola Singapore in 2014 is a manifestation of the broader trend toward the domestication of drone technologies within city spaces, indicating a prolonged desire to eschew its relationship to violence. This article seeks to briefly provide one interpretation into this ad and the broader contextual implications of drones in cities. We argue that while a variety of strategies are clearly deployed within this ad—the redesign of the body of the drone, the attempt to negate the relation between drones and violence, and finally, through the reconfiguration of the drone eye from the eye that ‘watches’ to the eye that ‘sees’—the overall implications of drone technologies within city spaces warrants further investigation. Particularly as drone technologies are easily adaptable to changing environments, often concealing its security and surveillance capabilities, commercial and domestic participants in this trend must be critically aware of the potential consequences underlying the normalisation of drones as part of everyday life in the city.
The Spectrum of Density Fluctuations of Noble Gases Probed by THz Neutron and X-ray Spectroscopy
Authors: Cunsolo Alessandro
Published: February 2016

Approximately 50 years of inelastic scattering studies of noble gases are reviewed to illustrate the main advances achieved in the understanding of the THz dynamics of simple systems. The gradual departure of the spectral shape from the hydrodynamic regime is discussed with an emphasis on the phenomenology of fast (sub-ps) relaxation processes. This review shows that relaxation phenomena in noble gases have an essentially collisional origin, which is also revealed by the parallelism between their characteristic timescale and the interatomic collision time. Additionally, recent THz spectroscopy results on noble gases at extreme thermodynamic conditions are discussed to illustrate the need for a revision of our current understanding of the supercritical phase.
Photonic Crystal-Based Sensors for Detecting Alcohol Concentration
Authors: Kuo Wen-Kai , Weng Hsueh-Ping , Hsu Jyun-Jheng , Yu Hsin
Published: February 2016

Polystyrene (PS) opal and titania (TiO2) inverse opal films were fabricated by the self-assembly colloidal crystal template technique. Based on Bragg’s law, these sensors were used to detect the different concentrations of ethanol solution. The results indicated that TiO2 inverse opal films were advantageous over PS opal film for detecting the ethanol concentration. TiO2 inverse opal films sintered at 600 °C retained the highest sensitivity for ethanol concentration identification, since the anatase phase was transformed into the rutile phase, which resulted in an enhancement of the refractive index, i.e., an increase in the amount of the red shift.
Second Order Stark-Effect Induced Gailitis Resonances in e + Ps and p + 7Li
Authors: Hu Chi , Papp Zoltan
Published: February 2016

We present a detailed comparison between the first order Stark-effect induced Gailitis resonance in e+ + H (n = 2) and the second order Stark-effect induced resonance in e + Ps (n = 1). Common characteristics as well as differences of these resonances will be identified. These results will be used to assess the presence of Gailitis resonances in the scattering of proton on the ground state of 7Li atom. During the lifetime of the Gailitis resonance, nuclear fusion is enhanced by the resonant entry of the proton into the nucleus of 7Li via a compound nuclear energy level of 8Be*.
Exercise Strengthens Central Nervous System Modulation of Pain in Fibromyalgia
Authors: Ellingson Laura , Stegner Aaron , Schwabacher Isaac , Koltyn Kelli , Cook Dane
Published: February 2016

To begin to elucidate the mechanisms underlying the benefits of exercise for chronic pain, we assessed the influence of exercise on brain responses to pain in fibromyalgia (FM). Complete data were collected for nine female FM patients and nine pain-free controls (CO) who underwent two functional neuroimaging scans, following exercise (EX) and following quiet rest (QR). Brain responses and pain ratings to noxious heat stimuli were compared within and between groups. For pain ratings, there was a significant (p < 0.05) Condition by Run interaction characterized by moderately lower pain ratings post EX compared to QR (d = 0.39–0.41) for FM but similar to ratings in CO (d = 0.10–0.26), thereby demonstrating that exercise decreased pain sensitivity in FM patients to a level that was analogous to pain-free controls. Brain responses demonstrated a significant within-group difference in FM patients, characterized by less brain activity bilaterally in the anterior insula following QR as compared to EX. There was also a significant Group by Condition interaction with FM patients showing less activity in the left dorsolateral prefrontal cortex following QR as compared to post-EX and CO following both conditions. These results suggest that exercise appeared to stimulate brain regions involved in descending pain inhibition in FM patients, decreasing their sensitivity to pain. Thus, exercise may benefit patients with FM via improving the functional capacity of the pain modulatory system.
Off-Design Modeling of Natural Gas Combined Cycle Power Plants: An Order Reduction by Means of Thermoeconomic Input–Output Analysis
Authors: Keshavarzian Sajjad , Gardumi Francesco , Rocco Matteo , Colombo Emanuela
Published: February 2016

In a European context characterized by growing need for operational flexibility across the electricity sector, the combined cycle power plants are increasingly subjected to cyclic operation. These new operation profiles cause an increase of production costs and decrease of revenues, which undermines the competitiveness of the combined cycles. Power plant operators need tools to predict the effect of off-design operation and control mechanisms on the performance of the power plant. Traditional Thermodynamic or Thermoeconomic models may be unpractical for the operators, due to their complexity and the computational effort they require. This study proposes a Thermoeconomic Input–Output Analysis model for the on- and off-design performance prediction of energy systems, and applies it to La Casella Natural Gas Combined Cycle (NGCC) power plant, in Italy. It represents a stand-alone, reduced order model, where the cost structure of the plant products and the Thermoeconomic performance indicators are derived for on- and off-design conditions as functions of the load and of different control mechanisms, independently from the Thermodynamic model. The results of the application show that the Thermoeconomic Input–Output Analysis model is a suitable tool for power plant operators, able to derive the same information coming from traditional Thermoeconomic Analysis with reduced complexity and computational effort.
Technical, Economical and Social Assessment of Photovoltaics in the Frame of the Net-Metering Law for the Province of Salta, Argentina
Authors: Ramirez Camargo Luis , Franco Judith , Sarmiento Babieri Nilsa , Belmonte Silvina , Escalante Karina , Pagany Raphaela , Dorner Wolfgang
Published: February 2016

Central and Northern Argentinean regions possess a high potential for the generation of solar energy. The realization of this potential is an alternative to alleviate the strong dependence on imports of fossil energy and to reduce the CO2 emissions of the country. However, the adoption of photovoltaics (PV) is still in an incipient state. It is undermined by a context of heavily subsidized electricity prices, high equipment and installation costs and a lack of information, training and experience in handling PV technology. This paper presents a techno-economical assessment of the application of the recently enacted net-metering law for promoting renewable energies (RE) in the Province of Salta (Northwest Argentina) for the case of PV. The assessment shows under which conditions and for which types of consumers it is profitable to adopt PV in the context of the law. This analysis is supported by a participatory planning approach as a study of stakeholders’ attitudes towards RE, intentions to adopt PV and their knowledge about the law. The results of this study and the economical analysis serve to provide recommendations aimed at increasing the level of PV adoption in the province.
A Dual-Consequent-Pole Vernier Memory Machine
Authors: Yang Hui , Lin Heyun , Zhu Zi-Qiang , Fang Shuhua , Huang Yunkai
Published: February 2016

This paper proposes a novel dual-consequent-pole Vernier memory machine (DCP-VMM) featuring alternatively arranged NdFeB and low coercive-force (LCF) magnet poles on the rotating and stationary sides, respectively. Due to the presence of LCF magnets that can be repetitively magnetized or demagnetized via a simple current pulse, the extra-high torque density at low-speed, and excellent high-efficient high-speed flux-weakening performance can be simultaneously realized. The configuration and operating principle, as well as the design considerations of the proposed machine are introduced, respectively. The finite element method (FEM) coupled with a nonlinear analytical hysteresis model for LCF magnets is employed to investigate the electromagnetic performance of the machine, which verifies the effectiveness of machine design and the feasibility as a competent candidate for automotive applications.
Monte Carlo Evaluation of the Impact of Subsequent Strokes on Backflashover Rate
Authors: Gatta Fabio , Geri Alberto , Lauria Stefano , Maccioni Marco
Published: February 2016

The paper deals with the impact of subsequent strokes on the backflashover rate (BFR) of HV overhead transmission lines (OHLs), assessed by means of an ATP-EMTP Monte Carlo procedure. The application to a typical 150 kV Italian OHL is discussed, simulating several tower grounding system arrangements. Subsequent strokes parameters are added to the statistical simulation variables: peak current, front time, time-to-half value, lightning polarity, line insulation withstand, lightning location and phase angle of the power frequency voltage. The input data are fed to an ATP-EMTP complete circuit model of the OHL, including line insulation, lightning representation and tower grounding system, the latter simulated by a pi-circuit model able to simulate the effects due to propagation and soil ionization, at modest computational costs. Numerical results evidence a non-negligible BFR increase (in relative terms) due to subsequent strokes: for spatially concentrated grounding systems the BFR increase approximatively vary in inverse proportion with the low frequency grounding resistance, whereas for spatially extended grounding systems the BFR increase depends on the grounding system behavior at high frequencies.
Recent Advances in Experimental Studies of Social Dilemma Games
Authors: Chaudhuri Ananish
Published: February 2016

I provide a broad overview of the findings reported in the articles submitted for this special volume on experimental studies of social dilemma problems. I start by providing a synopsis of where current research stands on this topic. Then I go on to discuss the specific papers and how those papers extend our knowledge in this area and add value to the current state of the art.
Incidence and Risk Factors of Thromboembolism with Multiple Myeloma in the Presence of Death as a Competing Risk: An Empirical Comparison of Statistical Methodologies
Authors: Brown Joshua , Adams Val
Published: February 2016

Multiple myeloma (MM) has an inherent high risk of thromboembolic events associated with patient as well as disease- and treatment-related factors. Previous studies have assessed the association of MM-related thromboembolism using “traditional” Kaplan–Meier (KM) and/or Cox proportional hazard (PH) regression. In the presence of high incidence of death, as would be the case in cancer patients with advanced age, these statistical models will produce bias estimates. Instead, a competing risk framework should be used. This study assessed the baseline patient demographic and clinical characteristics associated with MM-related thromboembolism and compared the cumulative incidence and the measures of association obtained using each statistical approach. The cumulative incidence of thromboembolism was 9.2% using the competing risk framework and nearly 12% using the KM approach. Bias in the measures of covariate risk associations was highest for factors related to risk of death such as increased age (75% bias) and severe liver disease (50%) for the Cox PH model compared to the competing risk model. These results show that correct specification of statistical techniques can have a large impact on the results obtained.
Response to Comments on Meo et al. Association of Exposure to Radio-Frequency Electromagnetic Field Radiation (RF-EMFR) Generated by Mobile Phone Base Stations with Glycated Hemoglobin (HbA1c) and Risk of Type 2 Diabetes Mellitus. Int. J. Environ. Res. Public Health, 2015, 12, 14519–14528
Authors: Meo Sultan , Alsubaie Yazeed , Almubarak Zaid , Almutawa Hisham , AlQasem Yazeed , Hasanato Rana
Published: February 2016

We highly appreciate the readers’ interest [1] in our article [2] titled “Association of Exposure to Radio-Frequency Electromagnetic Field Radiation (RF-EMFR) Generated by Mobile Phone Base Stations with Glycated Hemoglobin (HbA1c) and Risk of Type 2 Diabetes Mellitus” published in the International Journal of Environmental Research and Public Health [2].[...]
Automated Collection of Real-Time Alerts of Citizens as a Useful Tool to Continuously Monitor Malodorous Emissions
Authors: Brattoli Magda , Mazzone Antonio , Giua Roberto , Assennato Giorgio , De Gennaro Gianluigi
Published: February 2016

The evaluation of odor emissions and dispersion is a very arduous topic to face; the real-time monitoring of odor emissions, the identification of chemical components and, with proper certainty, the source of annoyance represent a challenge for stakeholders such as local authorities. The complaints of people, often not systematic and variously distributed, in general do not allow us to quantify the perceived annoyance. Experimental research has been performed to detect and evaluate olfactory annoyance, based on field testing of an innovative monitoring methodology grounded in automatic recording of citizen alerts. It has been applied in Taranto, in the south of Italy where a relevant industrial area is located, by using Odortel® for automated collection of citizen alerts. To evaluate its reliability, the collection system has been integrated with automated samplers, able to sample odorous air in real time, according to the citizen alerts of annoyance and, moreover, with meteorological data (especially the wind direction) and trends in odor marker compounds, recorded by air quality monitoring stations. The results have allowed us, for the first time, to manage annoyance complaints, test their reliability, and obtain information about the distribution and entity of the odor phenomena, such that we were able to identify, with supporting evidence, the source as an oil refinery plant.
Modulating Astrocyte Transition after Stroke to Promote Brain Rescue and Functional Recovery: Emerging Targets Include Rho Kinase
Authors: Abeysinghe Hima , Phillips Ellie , Chin-Cheng Heung , Beart Philip , Roulston Carli
Published: February 2016

Stroke is a common and serious condition, with few therapies. Whilst previous focus has been directed towards biochemical events within neurons, none have successfully prevented the progression of injury that occurs in the acute phase. New targeted treatments that promote recovery after stroke might be a better strategy and are desperately needed for the majority of stroke survivors. Cells comprising the neurovascular unit, including blood vessels and astrocytes, present an alternative target for supporting brain rescue and recovery in the late phase of stroke, since alteration in the unit also occurs in regions outside of the lesion. One of the major changes in the unit involves extensive morphological transition of astrocytes resulting in altered energy metabolism, decreased glutamate reuptake and recycling, and retraction of astrocyte end feed from both blood vessels and neurons. Whilst globally inhibiting transitional change in astrocytes after stroke is reported to result in further damage and functional loss, we discuss the available evidence to suggest that the transitional activation of astrocytes after stroke can be modulated for improved outcomes. In particular, we review the role of Rho-kinase (ROCK) in reactive gliosis and show that inhibiting ROCK after stroke results in reduced scar formation and improved functional recovery.
Biochemical Characterization and Complete Conversion of Coenzyme Specificity of Isocitrate Dehydrogenase from Bifidobacterium longum
Authors: Huang Shi-Ping , Cheng Hong-Mei , Wang Peng , Zhu Guo-Ping
Published: February 2016

Bifidobacterium longum is a very important gram-positive non-pathogenic bacterium in the human gastrointestinal tract for keeping the digestive and immune system healthy. Isocitrate dehydrogenase (IDH) from B. longum (BlIDH), a novel member in Type II subfamily, was overexpressed, purified and biochemically characterized in detail. The active form of BlIDH was an 83-kDa homodimer. Kinetic analysis showed BlIDH was a NADP+-dependent IDH (NADP-IDH), with a 567- and 193-fold preference for NADP+ over NAD+ in the presence of Mg2+ and Mn2+, respectively. The maximal activity for BlIDH occurred at 60 °C (with Mn2+) and 65 °C (with Mg2+), and pH 7.5 (with Mn2+) and pH 8.0 (with Mg2+). Heat-inactivation profiles revealed that BlIDH retained 50% of maximal activity after incubation at 45 °C for 20 min with either Mn2+ or Mg2+. Furthermore, the coenzyme specificity of BlIDH can be completely reversed from NADP+ to NAD+ by a factor of 2387 by replacing six residues. This current work, the first report on the coenzyme specificity conversion of Type II NADP-IDHs, would provide better insight into the evolution of NADP+ use by the IDH family.
Identification of Human Adenovirus in Respiratory Samples with Luminex Respiratory Virus Panel Fast V2 Assay and Real-Time Polymerase Chain Reaction
Authors: Esposito Susanna , Scala Alessia , Bianchini Sonia , Zampiero Alberto , Fossali Emilio , Principi Nicola
Published: February 2016

In order to compare the last version of the Respiratory Virus Panel (RVP) Fast assay for human Adenovirus (hAdv) detection with a specific real-time polymerase chain reaction (qPCR), which is considered the gold standard for hAdv detection, nasopharyngeal samples collected from 309 children (age range, four months to eight years) with respiratory tract infection were tested using the RVP Fast v2 assay (Luminex Molecular Diagnostics, Inc., Toronto, ON, Canada) and a specific TaqMan qPCR to identify hAdv DNA. The RVP Fast v2 assay detected 30/61 (49.2%) hAdv infections that had been identified by real-time qPCR, demonstrating a significantly lower detection rate (p < 0.001). The sensitivity of the RVP Fast v2 assay in comparison to qPCR was lower in younger children (42.9% vs. 57.7%; Cohen’s kappa coefficient, 0.53); in samples with co-infections (40.0% vs. 56.7%; Cohen’s kappa coefficient, 0.52); and in samples with hAdv type C (45.9% vs. 57.1%; Cohen’s kappa coefficient, 0.60). Samples with lower viral loads were associated with a significantly lower sensitivity of the RVP Fast v2 assay (35.1% vs. 68.2%, p = 0.01; Cohen’s kappa coefficients, 0.49). The RVP Fast v2 assay has important limitations for the detection of hAdv and cannot be used to evaluate whether hAdvs are the main etiologic agent responsible for an outbreak or when epidemiological studies are performed.
Reduced Connexin26 in the Mature Cochlea Increases Susceptibility to Noise-Induced Hearing Loss in Mice
Authors: Zhou Xing-Xing , Chen Sen , Xie Le , Ji Yu-Zi , Wu Xia , Wang Wen-Wen , Yang Qi , Yu Jin-Tao , Sun Yu , Lin Xi , Kong Wei-Jia
Published: February 2016

Connexin26 (Cx26, encoded by GJB2) mutations are the most common cause of non-syndromic deafness. GJB2 is thought to be involved in noise-induced hearing loss (NIHL). However, the role of Cx26 in NIHL is still obscure. To explore the association between Cx26 and NIHL, we established a Cx26 knockdown (KD) mouse model by conditional knockdown of Cx26 at postnatal day 18 (P18), and then we observed the auditory threshold and morphologic changes in these mice with or without noise exposure. The Cx26 KD mice did not exhibit substantial hearing loss and hair cell degeneration, while the Cx26 KD mice with acoustic trauma experienced higher hearing loss than simple noise exposure siblings and nearly had no recovery. Additionally, extensive outer hair cell loss and more severe destruction of the basal organ of Corti were observed in Cx26 KD mice after noise exposure. These data indicate that reduced Cx26 expression in the mature mouse cochlea may increase susceptibility to noise-induced hearing loss and facilitate the cell degeneration in the organ of Corti.
Co-Production of Fungal Biomass Derived Constituents and Ethanol from Citrus Wastes Free Sugars without Auxiliary Nutrients in Airlift Bioreactor
Authors: Satari Behzad , Karimi Keikhosro , Taherzadeh Mohammad , Zamani Akram
Published: February 2016

The potential of two zygomycetes fungi, Mucor indicus and Rhizopus oryzae, in assimilating citrus waste free sugars (CWFS) and producing fungal chitosan, oil, and protein as well as ethanol was investigated. Extraction of free sugars from citrus waste can reduce its environmental impact by decreasing the possibility of wild microorganisms growth and formation of bad odors, a typical problem facing the citrus industries. A total sugar concentration of 25.1 g/L was obtained by water extraction of citrus waste at room temperature, used for fungal cultivation in shake flasks and airlift bioreactor with no additional nutrients. In shake flasks cultivations, the fungi were only able to assimilate glucose, while fructose remained almost intact. In contrast, the cultivation of M. indicus and R. oryzae in the four-liter airlift bioreactor resulted in the consumption of almost all sugars and production of 250 and 280 g fungal biomass per kg of consumed sugar, respectively. These biomasses correspondingly contained 40% and 51% protein and 9.8% and 4.4% oil. Furthermore, the fungal cell walls, obtained after removing the alkali soluble fraction of the fungi, contained 0.61 and 0.69 g chitin and chitosan per g of cell wall for M. indicus and R. oryzae, respectively. Moreover, the maximum ethanol yield of 36% and 18% was obtained from M. indicus and R. oryzae, respectively. Furthermore, that M. indicus grew as clump mycelia in the airlift bioreactor, while R. oryzae formed spherical suspended pellets, is a promising feature towards industrialization of the process.
The Translational Genomics Core at Partners Personalized Medicine: Facilitating the Transition of Research towards Personalized Medicine
Authors: Blau Ashley , Brown Alison , Mahanta Lisa , Amr Sami
Published: February 2016

The Translational Genomics Core (TGC) at Partners Personalized Medicine (PPM) serves as a fee-for-service core laboratory for Partners Healthcare researchers, providing access to technology platforms and analysis pipelines for genomic, transcriptomic, and epigenomic research projects. The interaction of the TGC with various components of PPM provides it with a unique infrastructure that allows for greater IT and bioinformatics opportunities, such as sample tracking and data analysis. The following article describes some of the unique opportunities available to an academic research core operating within PPM, such the ability to develop analysis pipelines with a dedicated bioinformatics team and maintain a flexible Laboratory Information Management System (LIMS) with the support of an internal IT team, as well as the operational challenges encountered to respond to emerging technologies, diverse investigator needs, and high staff turnover. In addition, the implementation and operational role of the TGC in the Partners Biobank genotyping project of over 25,000 samples is presented as an example of core activities working with other components of PPM.
A Review of Transfer Films and Their Role in Ultra-Low-Wear Sliding of Polymers
Authors: Ye Jiaxin , Burris David , Xie Ting
Published: February 2016

In dry sliding conditions, polytetrafluoroethylene (PTFE) composites can form thin, uniform, and protective transfer films on hard, metallic counterfaces that may play a significant role in friction and wear control. Qualitative characterizations of transfer film morphology, composition, and adhesion to the counterface suggest they are all good predictors of friction and, particularly, wear performance. However, a lack of quantitative transfer film characterization methods and uncertainty regarding specific mechanisms of friction and wear control make definitive conclusions about causal relationships between transfer film and tribological properties difficult. This paper reviews the state of the art in the solid lubricant transfer film literature and highlights recent advances in quantitative characterization thereof.
Effectiveness of Fiber Reinforcement on the Mechanical Properties and Shrinkage Cracking of Recycled Fine Aggregate Concrete
Authors: Nam Jeongsoo , Kim Gyuyong , Yoo Jaechul , Choe Gyeongcheol , Kim Hongseop , Choi Hyeonggil , Kim Youngduck
Published: February 2016

This paper presents an experimental study conducted to investigate the effect of fiber reinforcement on the mechanical properties and shrinkage cracking of recycled fine aggregate concrete (RFAC) with two types of fiber—polyvinyl alcohol (PVA) and nylon. A small fiber volume fraction, such as 0.05% or 0.1%, in RFAC with polyvinyl alcohol or nylon fibers was used for optimum efficiency in minimum quantity. Additionally, to make a comparative evaluation of the mechanical properties and shrinkage cracking, we examined natural fine aggregate concrete as well. The test results revealed that the addition of fibers and fine aggregates plays an important role in improving the mechanical performance of the investigated concrete specimens as well as controlling their cracking behavior. The mechanical properties such as compressive strength, splitting tensile strength, and flexural strength of fiber-reinforced RFAC were slightly better than those of non-fiber-reinforced RFAC. The shrinkage cracking behavior was examined using plat-ring-type and slab-type tests. The fiber-reinforced RFAC showed a greater reduction in the surface cracks than non-fiber-reinforced concrete. The addition of fibers at a small volume fraction in RFAC is more effective for drying shrinkage cracks than for improving mechanical performance.
Newly Developed Techniques on Polycondensation, Ring-Opening Polymerization and Polymer Modification: Focus on Poly(Lactic Acid)
Authors: Hu Yunzi , Daoud Walid , Cheuk Kevin , Lin Carol
Published: February 2016

Polycondensation and ring-opening polymerization are two important polymer synthesis methods. Poly(lactic acid), the most typical biodegradable polymer, has been researched extensively from 1900s. It is of significant importance to have an up-to-date review on the recent improvement in techniques for biodegradable polymers. This review takes poly(lactic acid) as the example to present newly developed polymer synthesis techniques on polycondensation and ring-opening polymerization reported in the recent decade (2005–2015) on the basis of industrial technique modifications and advanced laboratory research. Different polymerization methods, including various solvents, heating programs, reaction apparatus and catalyst systems, are summarized and compared with the current industrial production situation. Newly developed modification techniques for polymer properties improvement are also discussed based on the case of poly(lactic acid).
Hexakis (propargyl-1H-tetrazole) Iron(II) X2 [X = BF4, ClO4]—Spin Switchable Complexes with Functionalization Potential and the Myth of the Explosive SCO Compound
Authors: Seifried Marco , Knoll Christian , Giester Gerald , Reissner Michael , Müller Danny , Weinberger Peter
Published: February 2016

Spin crossover complexes with ligands suitable for further functionalization could allow for an easy access to multifunctional switchable materials. Within this context, we recently characterized the Fe(II) SCO complexes of propargyl-1H-tetrazole. Although the design of the ligand seems similar to the one of the well-known propyl-1H-tetrazole, the spin transition behavior is notably different. Both compounds reported herein feature a gradual spin transition with a T1/2 shifted to lower temperatures, compared to the more flexible propyl-ligand. Due to the potential instable structure of these compounds, especially of the perchlorate complex, we also investigated our compounds with regard to their sensitivity against thermal and mechanical stress. These experiments led to the conclusion that the proper handling includes no enhanced risk of explosive decomposition.
Metabolomic Profiling of Submaximal Exercise at a Standardised Relative Intensity in Healthy Adults
Authors: Muhsen Ali Ali , Burleigh Mia , Daskalaki Evangelia , Zhang Tong , Easton Chris , Watson David
Published: February 2016

Ten physically active subjects underwent two cycling exercise trials. In the first, aerobic capacity (VO2max) was determined and the second was a 45 min submaximal exercise test. Urine samples were collected separately the day before (day 1) , the day of (day 2), and the day after (day 3) the submaximal exercise test (12 samples per subject). Metabolomic profiling of the samples was carried out using hydrophilic interaction chromatography (HILIC) coupled to an Orbitrap Exactive mass spectrometer. Data were extracted, database searched and then subjected to principle components (PCA) and orthogonal partial least squares (OPLSDA) modelling. The best results were obtained from pre-treating the data by normalising the metabolites to their mean output on days 1 and 2 of the trial. This allowed PCA to separate the day 2 first void samples (D2S1) from the day 2 post-exercise samples (D2S3) PCA also separated the equivalent samples obtained on day 1 (D1S1 and D1S3). OPLSDA modelling separated both the D2S1 and D2S3 samples and D1S1 and D1S3 samples. The metabolites affected by the exercise samples included a range of purine metabolites and several acyl carnitines. Some metabolites were subject to diurnal variation these included bile acids and several amino acids, the variation of these metabolites was similar on day 1 and day 2 despite the exercise intervention on day 2. Using OPLS modelling it proved possible to identify a single abundant urinary metabolite provisionally identified as oxo-aminohexanoic acid (OHA) as being strongly correlated with VO2max when the levels in the D2S3 samples were considered.
Mathematical Modelling and Simulation Research of Thermal Engraving Technology Based on PMMA Material
Authors: Han Xiaowei , Liu Xiaowei , Tian Li
Published: February 2016

We proposed a thermal engraving technology based on heat transfer theory and polymer rheology in microfluidic field. Then, we established a 3D model of the thermal engraving process based on polymethyl methacrylate (PMMA) material. We could employ the model to analyze the influence of temperature and speed on microchannel processing through the finite element simulation. Thus, we gained the optimal processing parameters. The orthogonal experiments were carried out within the parameter ranges obtained by the simulation results. Finally, we fabricated the smooth microchannel, the average roughness of which was 0.3 μm, by using the optimal parameters. Furthermore, we examined the surface morphology and wettability. Our work provides a convenient technological support for a fast, low-cost, and large-scale manufacturing method of microfluidic chips.
N-doped TiO2 Nanotubes as an Effective Additive to Improve the Catalytic Capability of Methanol Oxidation for Pt/Graphene Nanocomposites
Authors: Wang Xiaohua , Li Yueming , Liu Shimin , Zhang Long
Published: February 2016

N-doped TiO2 nanotubes have been prepared as additives to improve the catalytic capability of Pt/graphene composites in methanol oxidation reactions. Electrochemical experiments show that the catalytic performance of Pt/graphene composites has been greatly improved by the introduction of N-doped TiO2 nanotubes.
Validation of Correction Algorithms for Near-IR Analysis of Human Milk in an Independent Sample Set—Effect of Pasteurization
Authors: Kotrri Gynter , Fusch Gerhard , Kwan Celia , Choi Dasol , Choi Arum , Al Kafi Nisreen , Rochow Niels , Fusch Christoph
Published: February 2016

Commercial infrared (IR) milk analyzers are being increasingly used in research settings for the macronutrient measurement of breast milk (BM) prior to its target fortification. These devices, however, may not provide reliable measurement if not properly calibrated. In the current study, we tested a correction algorithm for a Near-IR milk analyzer (Unity SpectraStar, Brookfield, CT, USA) for fat and protein measurements, and examined the effect of pasteurization on the IR matrix and the stability of fat, protein, and lactose. Measurement values generated through Near-IR analysis were compared against those obtained through chemical reference methods to test the correction algorithm for the Near-IR milk analyzer. Macronutrient levels were compared between unpasteurized and pasteurized milk samples to determine the effect of pasteurization on macronutrient stability. The correction algorithm generated for our device was found to be valid for unpasteurized and pasteurized BM. Pasteurization had no effect on the macronutrient levels and the IR matrix of BM. These results show that fat and protein content can be accurately measured and monitored for unpasteurized and pasteurized BM. Of additional importance is the implication that donated human milk, generally low in protein content, has the potential to be target fortified.
Abiotic Raw-Materials in Life Cycle Impact Assessments: An Emerging Consensus across Disciplines
Authors: Drielsma Johannes , Allington Ruth , Brady Thomas , Guinée Jeroen , Hammarstrom Jane , Hummen Torsten , Russell-Vaccari Andrea , Schneider Laura , Sonnemann Guido , Weihed Pär
Published: February 2016

This paper captures some of the emerging consensus points that came out of the workshop “Mineral Resources in Life Cycle Impact Assessment: Mapping the path forward”, held at the Natural History Museum London on 14 October 2015: that current practices rely in many instances on obsolete data, often confuse resource depletion with impacts on resource availability, which can therefore provide inconsistent decision support and lead to misguided claims about environmental performance. Participants agreed it would be helpful to clarify which models estimate depletion and which estimate availability, so that results can be correctly reported in the most appropriate framework. Most participants suggested that resource availability will be more meaningfully addressed within a comprehensive Life Cycle Sustainability Assessment framework rather than limited to an environmental Life Cycle Assessment or Footprint. Presentations from each of the authors are available for download [1].
High-Frequency Financial Econometrics
Authors: Thompson Harley
Published: February 2016

This book is fundamentally about the estimation of risk.[...]
National-Scale Estimates of Ground-Level PM2.5 Concentration in China Using Geographically Weighted Regression Based on 3 km Resolution MODIS AOD
Authors: You Wei , Zang Zengliang , Zhang Lifeng , Li Yi , Pan Xiaobin , Wang Weiqi
Published: February 2016

High spatial resolution estimating of exposure to particulate matter 2.5 (PM2.5) is currently very limited in China. This study uses the newly released nationwide, hourly PM2.5 concentrations to create a nationwide, geographically weighted regression (GWR) model to estimate ground-level PM2.5 concentrations in China. A3 km resolution aerosol optical depth (AOD) product from MODIS is used as the primary predictor. Fire emissions detected by MODIS fire count were considered in the model development process. Additionally, meteorological features were used as covariates in the model to improve the estimation of ground-level PM2.5 concentrations. The model performed well and explained 81% of the daily PM2.5 concentration variations in model predictions, and the cross validations R2 is 0.79. The cross-validated root mean squared error (RMSE) of the model was 18.6 μg/m3.Annual PM2.5 concentrations retrieved by the MODIS 3 km AOD product indicated that most of the residential community areas exceeded the new annual Chinese PM2.5 National Standard level 2. Estimated high-resolution national-scale daily PM2.5 maps are useful to identify severe air pollution episodes and determine health risk assessments. These results suggest that this approach is useful for estimating large-scale ground-level PM2.5 distributions, especially for regions without PM monitoring sites.