bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparative Chloroplast Genome Analyses of Six Hemlock Trees in East Asia: Insights into Their Genomic Characterization and Phylogenetic Relationship
Authors: Chen Lin , Liu Xin , Wang Zhibei , Wu Xi , Hong Kaiyue , Xie Chunping
Published: October 2023

Hemlocks (Pinaceae: Tsuga) are widely distributed in North America and East Asia, forming a reticulate evolutionary structure in East Asia with significant ecological importance. To clarify the chloroplast genome characteristics and phylogenetic relationships among Tsuga species, we analyzed the chloroplast genomes of T. chinensis var. tchekiangensis, T. chinensis, T. diversifolia, T. dumosa, T. forrestii, and T. sieboldii, performing associated phylogenetic analyses. The results reveal that the chloroplast genome lengths among the six Tsuga species vary from 120,520 to 121,010 bp, encompassing about 108 to 112 genes, including 35/32 tRNA genes and 4 rRNA genes. A codon usage analysis highlighted a preference for A/U-ending codons, and all six nucleotide types have A/T bases and a prevalence of mononucleotides. Notably, all Tsuga species exhibit inverted repeat (IR) contractions and possess unique hexanucleotides absent in the other species of Pinaceae, potentially making them more susceptible to gene recombination or rearrangement during evolution. While most variations are observed in non-coding regions, particularly in intergenic fragments, substantial variation sites are also present within the genes. The phylogenetic tree, constructed using chloroplast genomes, substantiates the sister taxa relationship between Tsuga and Nothotsuga. Furthermore, it confirms that T. chinensis var. tchekiangensis exhibits a closer relationship with T. forrestii than with T. chinensis. These findings not only provide partial evidence that T. chinensis may not constitute a monophyletic species but also underscore the necessity of reevaluating the taxonomic status of T. chinensis var. tchekiangensis. In addition, while the RSCU cluster analysis is basically consistent with the phylogenetic analysis, it also highlights a distinct differentiation between Nothotsuga and Tsuga. This study not only provides molecular-level phylogenetic classification evidence of Pinaceous genera via chloroplast genome analyses but also offers compelling evidence for further exploring the relationships and species delimitation among the hemlocks of East Asia.
Evaluating the Stand Structure, Carbon Sequestration, Oxygen Release Function, and Carbon Sink Value of Three Artificial Shrubs alongside the Tarim Desert Highway
Authors: Li Lin , Zayiti Abudoukeremujiang , He Xuemin
Published: October 2023

Currently, the ecological problems caused by the greenhouse effect are growing more serious, and implementing carbon sequestration methods is an effective way to address them. Arid and semi-arid desert areas have tremendous potential as carbon sinks, and artificial forests in these areas play an important role in absorbing and sequestering carbon dioxide. This study selected three main species of artificial protective trees along the Tarim Desert Highway—Haloxylon ammodendron (C.A.Mey.) Bunge, Calligonum mongolicum Turcz. and Tamarix chinensis Lour.—and evaluated them for their carbon sequestration, oxygen release capacity, and economic benefits using Pn (net photosynthetic rate) and biomass methods. The results showed that the average daily Pn value and carbon sequestration and oxygen release per unit leaf area of T. chinensis were significantly higher than those of H. ammodendron and C. mongolicum (p < 0.05). The total carbon storage of the three shelterbelts was 15.41 × 104 t, and the carbon storage of H. ammodendron was significantly higher than that of C. mongolicum and T. chinensis (p < 0.05). According to the net photosynthetic rate method, the annual carbon sequestration and oxygen release of the shelter forest is 6.13 × 104 t a−1, and the transaction price is CNY 13.73 million a−1. The total amount of carbon sequestration and oxygen release of the shelter forest obtained via the biomass method is 97.61 × 104 t, and the transaction price is CNY 218.77 million. This study conducted research on the carbon sequestration capacity of protective forests along the Tarim Desert Highway located in an extremely arid region. It highlights the significant contribution of these protective forests in terms of carbon storage, playing a crucial role in promoting ecological restoration and sustainable development in arid areas. Additionally, this study provides a scientific basis for estimating carbon storage and promoting the sustainable management of artificial forests in arid desert regions.
Dietary NDF/Starch Ratio Modulates Colonic Microbiota and Metabolites in Lambs before and after Weaning
Authors: Han Xiaoxia , Zhao Haibi , Liu Guohua , Lv Feng , Pang Xin , Yang Fan , Wang Xiaojuan
Published: October 2023

The neutral detergent fiber (NDF)/starch ratio is a key determinant of the carbohydrate composition in ruminant diets, which affects the development of the gastrointestinal tract and animal growth. In this study, we used a combination of 16S amplicon sequencing and metabolomics technologies to reveal changes in the microbiota and their metabolites associated with digestive matter in the colon of lambs between different starter NDF/starch ratios and before and after weaning. A total of 40 male lambs of Hu sheep with a newborn weight of 3.14 ± 0.05 kg were selected for the experiment and fed with breast milk until 10 days of age, and were randomly divided into 2 groups, which were fed ad libitum with a starter of NDF/starch of 0.5 (A) or 1.0 (B) for a period of 56 days, and then weaned off the milk powder at 35 days of age. Six lambs with weights close to the average weight of the group were selected for slaughter in each of the two groups before (35 days of age) and after weaning (56 days of age), and were accordingly named A35, A56, B35 and B56. The results showed that, before weaning, the concentrations of acetic acid, propionic acid, butyric acid and total volatile fatty acids (TVFA) in group B35 were significantly higher than those in group A35 (p < 0.05), while the concentrations of isobutyric acid, valeric acid and isovaleric acid were significantly lower than those in group A35 (p < 0.05). After weaning, the concentrations of all volatile acids and total acids in group B56 were significantly lower than those in group A56 (p < 0.01). At the phylum level, the dominant phyla identified were Firmicutes and Bacteroidetes; the relative abundances of Firmicutes and Desulfobacterota were significantly higher and lower in group B35 than in group A35, respectively (p < 0.05); the relative abundances of Euryarchaeota and Desulfobacterota were significantly higher and lower in group A56 than in group A35 (p < 0.05); and, at the genus level, the relative abundance of Lachnospiraceae_FCS020_group and Eubacterium nodatum group had higher relative abundance in group B35 before weaning (p < 0.05). Metabolomic results showed that feeding 1.0 NDF/starch ratio starter before weaning significantly up-regulated (p < 0.05) the concentrations of several anti-inflammatory-related metabolites such as lithocholic acid, oleanolic acid and LysoP. After weaning, the number of differential microorganisms and anti-inflammatory-related metabolites decreased between the two ratios. In summary, feeding a 1.0 NDF/starch ratio starter may be more effective in regulating microbial fermentation, leading to an increase in beneficial microbiota and metabolites, thus improving colonic environmental homeostasis in lambs before and after weaning.
Enzymatic Hydrolysis of Kraft and Sulfite Pulps: What Is the Best Cellulosic Substrate for Industrial Saccharification?
Authors: Shevchenko Aleksandr R. , Mayorova Ksenia A. , Chukhchin Dmitry G. , Malkov Alexey V. , Toptunov Evgeniy A. , Telitsin Vadim D. , Rozhkova Aleksandra M. , Zorov Ivan N. , Rodicheva Maria A. , Plakhin Vadim A. , Akishin Denis A. , Poshina Daria N. , Semenova Margarita V. , Aksenov Andrey S. , Sinitsyn Arkady P.
Published: October 2023

Sulfite and kraft pulping are two principal methods of industrial delignification of wood. In recent decades, those have been considered as possibilities to pretreat recalcitrant wood lignocellulosics for the enzymatic hydrolysis of polysaccharides and the subsequent fermentation of obtained sugars to valuable bioproducts. Current work compares chemistry and technological features of two different cooking processes in the preparation of polysaccharide substrates for deep saccharification with P. verruculosum glycosyl hydrolases. Bleached kraft and sulfite pulps were subjected to hydrolysis with enzyme mixture of high xylanase, cellobiohydrolase, and β-glucosidase activities at a dosage of 10 FPU/g of dry pulp and fiber concentration of 2.5, 5, and 10%. HPLC was used to analyze soluble sugars after hydrolysis and additional acid inversion of oligomers to monosaccharides. Kraft pulp demonstrated higher pulp conversion after 48 h (74–99%), which mostly resulted from deep xylan hydrolysis. Sulfite-pulp hydrolysates, obtained in similar conditions due to higher hexose concentration (more than 50 g/L), had higher fermentability for industrial strains producing alcohols, microbial protein, or organic acids. Along with saccharification, enzymatic modification of non-hydrolyzed residues occurred, which led to decreased degree of polymerization and composition changes in two industrial pulps. As a result, crystallinity of kraft pulp increased by 1.3%, which opens possibilities for obtaining new types of cellulosic products in the pulp and paper industry. The high adaptability and controllability of enzymatic and fermentation processes creates prospects for the modernization of existing factories.
Bioprotection Efficiency of Metschnikowia Strains in Synthetic Must: Comparative Study and Metabolomic Investigation of the Mechanisms Involved
Authors: Puyo Maëlys , Mas Perrine , Roullier-Gall Chloé , Romanet Rémy , Lebleux Manon , Klein Géraldine , Alexandre Hervé , Tourdot-Maréchal Raphaëlle
Published: October 2023

Three Metschnikowia strains marketed as bioprotection yeasts were studied to compare their antimicrobial effect on a mixture of two Hanseniaspora yeast strains in synthetic must at 12 °C, mimicking pre-fermentative maceration by combining different approaches. The growth of the different strains was monitored, their nitrogen and oxygen requirements were characterised, and their metabolomic footprint in single and co-cultures studied. Only the M. fructicola strain and one M. pulcherrima strains colonised the must and induced the rapid decline of Hanseniaspora. The efficiency of these two strains followed different inhibition kinetics. Furthermore, the initial ratio between Metschnikowia and Hanseniaspora was an important factor to ensure optimal bioprotection. Nutrient consumption kinetics showed that apiculate yeasts competed with Metschnikowia strains for nutrient accessibility. However, this competition did not explain the observed bioprotective effect, because of the considerable nitrogen content remaining on the single and co-cultures. The antagonistic effect of Metschnikowia on Hanseniaspora probably implied another form of amensalism. For the first time, metabolomic analyses of the interaction in a bioprotection context were performed after the pre-fermentative maceration step. A specific footprint of the interaction was observed, showing the strong impact of the interaction on the metabolic modulation of the yeasts, especially on the nitrogen and vitamin pathways.
Application of Near-Infrared Spectroscopy and Fuzzy Improved Null Linear Discriminant Analysis for Rapid Discrimination of Milk Brands
Authors: Wu Xiaohong , Fang Yiheng , Wu Bin , Liu Man
Published: October 2023

The quality of milk is tightly linked to its brand. A famous brand of milk always has good quality. Therefore, this study seeks to design a new fuzzy feature extraction method, called fuzzy improved null linear discriminant analysis (FiNLDA), to cluster the spectra of collected milk for identifying milk brands. To elevate the classification accuracy, FiNLDA was applied to process the near-infrared (NIR) spectra of milk acquired by the portable near-infrared spectrometer. The principal component analysis and Savitzky–Golay (SG) filtering algorithm were employed to lower dimensionality and eliminate noise in this system, respectively. Thereafter, improved null linear discriminant analysis (iNLDA) and FiNLDA were applied to attain the discriminant information of the NIR spectra. At last, the K-nearest neighbor classifier was utilized for assessing the performance of the identification system. The results indicated that the maximum classification accuracies of LDA, iNLDA and FiNLDA were 74.7%, 88% and 94.67%, respectively. Accordingly, the portable NIR spectrometer in combination with FiNLDA can classify milk brands correctly and effectively.
Sustainable Development Solutions: Growing and Processing Raspberries on Lithuanian Farms
Authors: Ispiryan Audrone , Giedraitis Algirdas , Sermuksnyte-Alesiuniene Kristina , Butu Marian , Atkociuniene Vilma , Butu Alina , Viskelis Jonas , Miceikiene Astrida
Published: October 2023

The EU’s goals by 2050 are to ensure food security, prevent bio-diversity loss, and strengthen the EU food system’s resilience. Recent scientific research and the situation in the global market show that the cultivation and processing of raspberries is currently completely unsustainable. This sector is experiencing a huge decline in Lithuania. Therefore, we chose the sustainability of raspberry growing (from farm) and processing (to fork) as an object. The aim of this article was (i) to analyze the raw material of the raspberry plant for product sustainable processing, (ii) to create a digital sustainability measurement model, and (iii) to present sustainable development solutions for effective raspberry growing and processing on Lithuanian farms using content and descriptive methods. This paper discusses how to help small raspberry growers and processors achieve sustainable economic, environmental, and social performance from field raw material to processed products. Analysis of the scientific literature has revealed qualitative and quantitative sustainability indicators for improving raspberry production. The assessment of the sustainability according to our created model revealed the (un)sustainable factors and the current situation in raspberry farms on a Likert scale from very unsustainable to very sustainable. Based on the evaluation we have determined sustainable development solutions. Raspberry growing and processing in Lithuania can contribute to environmental conservation, economic growth, and social well-being, fostering a more sustainable and resilient agricultural sector by investing in R&D, improving productivity, creating employment opportunities and supporting rural communities, establishing a robust waste management system, and embracing renewable energy sources. Raspberry growers and processors can use the digital model we created for the sustainability, efficiency, and development directions of their farm.
Chemical–Physical Properties of Red Palm Oils and Their Application in the Manufacture of Aerated Emulsions with Improved Whipping Capabilities
Authors: Gao Ziwei , Zhu Yun , Jin Jun , Jin Qingzhe , Wang Xingguo
Published: October 2023

Red palm oil (RPO), which is rich in micronutrients, especially carotenoids, is different from its deodorized counterpart, palm oil. It is considered as one of the most promising food ingredients, owing to its unique compositions and nutritional values, while its usage could be further developed by improving its thermal behaviors. In this article, two typical commercial RPOs, HRPO (H. red palm oil) and NRPO (N. red palm oil), were evaluated by analyzing their fatty acids, triacylglycerols, micronutrients, oxidative stability index (OSI), and solid fat contents (SFCs). Micronutrients, mainly carotenes, tocopherols, polyphenols, and squalene, significantly increased the oxidative stability indices (OSIs) of the RPOs (from 10.02 to 12.06 h), while the OSIs of their micronutrient-free counterparts were only 1.12 to 1.82 h. HRPO exhibited a lower SFC than those of NRPO. RPOs softened at around 10 °C and completely melted near 20 °C. Although the softening problem may limit the usages of RPOs, that problem could be solved by incorporating RPOs with mango kernel fat (MKF). The binary blends containing 40% RPOs and 60% MKF exhibited desirable compatibilities, making that blend suitable for the manufacture of aerated emulsions with improved whipping performance and foam stabilities. The results provide a new application of RPOs and MKF in the manufacture of aerated emulsions with improved nutritional values and desired whipping capabilities.
Tannic Acid and Ca2+ Double-Crosslinked Alginate Films for Passion Fruit Preservation
Authors: Yang Jun , Fei Tao , Zhang Wanli , Cong Xinli
Published: October 2023

In this study, the interaction of different concentrations of tannic acid (TA) (10%, 20%, and 30% w/w) and Ca2+ with alginate (SA) was utilized to create double-crosslinked SA films. The resulting films were evaluated for their optical, mechanical, water resistance, and barrier properties, and their microstructure and intermolecular interactions were also characterized. The SA films containing 20% TA showed the best mechanical properties, with an observed increase in tensile strength of 22.54%. In terms of water vapor permeability, the SA film containing 30% TA exhibited the highest barrier property, which was 25.36% higher than that of the pure SA film. Moreover, TA demonstrated a strong UV absorption ability, resulting in a nearly 0% UV transmittance of the SA film at 280 nm. It can be seen that SA films containing 20% TA have excellent barrier and mechanical properties, and the development of such films will be applied to the storage and packaging of fresh food. It is worth noting that this work also investigated the effect of SA coatings containing different concentrations of TA on the preservation of passion fruits for 7 days. The results revealed that passion fruits treated with SA coatings containing a 30% TA concentration maintained a better appearance on the 7th day and had the lowest weight loss and crumpling indices of approximately 8.98% and 2.17, respectively, compared to the other treatment groups. Therefore, based on the overall results, the addition of 30% TA to SA coatings proved to be more effective and can be considered a promising approach for delaying fruit senescence and decay.
Mild Solutions of Fractional Integrodifferential Diffusion Equations with Nonlocal Initial Conditions via the Resolvent Family
Authors: Mu Jia , Yuan Zhiyuan , Zhou Yong
Published: October 2023

Fractional integrodifferential diffusion equations play a significant role in describing anomalous diffusion phenomena. In this paper, we study the existence and uniqueness of mild solutions to these equations. Firstly, we construct an appropriate resolvent family, through which the related equicontinuity, strong continuity, and compactness properties are studied using the convolution theorem of Laplace transform, the probability density function, the Cauchy integral formula, and the Fubini theorem. Then, we construct a reasonable mild solution for the considered equations. Finally, we obtain some sufficient conditions for the existence and uniqueness of mild solutions to the considered equations by some fixed point theorems.
An Assessment of the Genetic Parameters of Boars’ Reproductive Traits
Authors: Krupa Emil , Krupová Zuzana , Žáková Eliška , Bauer Jiří , Moravčíková Nina , Vrtková Irena
Published: October 2023

The aim of this study was to estimate genetic parameters for the reproductive traits of boars based on single-nucleotide polymorphism data. A total of 109,836 semen samples from 2249 boars were collected between 2010 and 2022. Five basic traits were assessed: sperm volume, sperm concentration, motility, number of abnormal sperm, and, for the first time for the local population, libido. In addition, two derived traits were assessed: total sperm count and number of functional sperm. Genetic parameters were estimated using the single-step genomic best linear unbiased prediction method (ssGBLUP). Dam and sire breeds were evaluated separately. The five basic traits were evaluated using five-trait models, while the two derived traits were evaluated using single-trait models. The heritability coefficients had lower values for all sperm quality traits with both methods. For the basic traits, the heritability ranged from 0.099 to 0.342. The greatest difference between dam and sire breeds was observed for the heritability of the sperm concentration trait (0.099 and 0.271, respectively). The heritability of the libido trait was twice as high for boars of sire breeds as it was for boars of dam breeds. The genetic parameters estimated with ssGBLUP can be used in routine genetic evaluations to improve the pig breeding process.
Genome-Wide Association Analysis across Endophenotypes in Alzheimer’s Disease: Main Effects and Disease Stage-Specific Interactions
Authors: Rosewood Thea , Nho Kwangsik , Risacher Shannon , Gao Sujuan , Shen Li , Foroud Tatiana , Saykin Andrew ,   on behalf of the Alzheimer’s Disease Neuroimaging Initiative
Published: October 2023

The underlying genetic susceptibility for Alzheimer’s disease (AD) is not yet fully understood. The heterogeneous nature of the disease challenges genetic association studies. Endophenotype approaches can help to address this challenge by more direct interrogation of biological traits related to the disease. AD endophenotypes based on amyloid-β, tau, and neurodegeneration (A/T/N) biomarkers and cognitive performance were selected from the Alzheimer’s Disease Neuroimaging Initiative (ADNI) cohort (N = 1565). A genome-wide association study (GWAS) of quantitative phenotypes was performed using an SNP main effect and an SNP by Diagnosis interaction (SNP × DX) model to identify disease stage-specific genetic effects. Nine loci were identified as study-wide significant with one or more A/T/N endophenotypes in the main effect model, as well as additional findings significantly associated with cognitive measures. These nine loci include SNPs in or near the genes APOE, SRSF10, HLA-DQB1, XKR3, and KIAA1671. The SNP × DX model identified three study-wide significant genetic loci (BACH2, EP300, and PACRG-AS1) with a neuroprotective effect in later AD stage endophenotypes. An endophenotype approach identified novel genetic associations and provided insight into the molecular mechanisms underlying the genetic associations that may otherwise be missed using conventional case-control study designs.
Interpersonal Characteristics and Binge Eating among Patients Pursuing Bariatric Surgery
Authors: Salameh-Dakwar Rawan , Elran-Barak Roni , Zahra-Zeitoun Yara , Soroka Gidon , Froylich Dvir , Assalia Ahmad , Latzer Yael
Published: October 2023

Background: Preoperative binge eating behavior has been associated with difficulties in weight loss maintenance among patients pursuing bariatric surgery. However, limited data exists on the relationship between interpersonal difficulties and binge eating. Objectives: To identify interpersonal factors linked with binge eating among bariatric surgery candidates. Setting: One hundred and seventeen adult bariatric surgery candidates (BMI = 42.2 ± 5.2) from three different hospitals completed questionnaires on the day of their bariatric committee meeting for operation approval. Methods: Binge eating was assessed using the Questionnaire on Eating and Weight Patterns-5 (QEWP-5) as a dichotomous variable. Self-esteem was measured using the Rosenberg Self-Esteem Scale (RSES), and interpersonal characteristics were evaluated using the short version of the Inventory of Interpersonal Problems (IIP-32). Sociodemographic variables (age, gender, income, education) and BMI were considered as confounders. Results: Approximately 25% of bariatric surgery candidates reported experiencing binge eating episodes within the previous three months. Participants with binge eating exhibited significantly lower self-esteem and more interpersonal difficulties, particularly in the domains of aggressiveness and dependence, compared to those without binge eating. Logistic regression analysis revealed that aggressiveness was a significant predictor of binge eating in this sample. Conclusions: This study is the first, to the best of our knowledge, to investigate the relationship between interpersonal difficulties and binge eating among bariatric surgery candidates. The findings highlight the significant contribution of aggressiveness to binge eating and emphasize the importance of clinicians assessing patients’ interpersonal functioning, particularly with regard to aggressiveness, as a factor that may contribute to the maintenance and occurrence of binge eating behaviors.
Psychological Help-Seeking Status for Living with Mental Health Conditions in Türkiye: Using a Multivariate Probit Model
Authors: Bayrakçeken Esra , Alkan Ömer , Abar Hayri
Published: October 2023

Background/aim: Mental disorders constitute a significant public health concern, contributing significantly to the overall disease burden. Individuals experiencing mental distress often delay or avoid seeking psychological help or do not seek help due to varying factors. This study examines the factors affecting individuals’ preferences, particularly young people, in seeking psychological help in Türkiye. Methods: This study utilized microdata from the Türkiye Health Survey conducted by TURKSTAT in 2016 and 2019. The factors influencing decisions to consult with a psychologist, psychotherapist, or psychiatrist were determined by multivariate probit regression analysis. The Türkiye Health Survey employed a stratified two-stage cluster sampling method, surveying 17,242 individuals over the age of 15 in 9470 households in 2016 and 17,084 individuals over the age of 15 in 9470 households in 2019. Results: The analysis findings indicated that, in the entire sample, women are more likely to seek psychological help, while younger women are less likely to do so. In the entire sample, as the education level increased, the probability of individuals receiving psychological help increased, while it decreased for young people. It was determined that people who use tobacco and alcohol are more likely to receive psychological help than others. It was also determined that individuals who perceive their health status as good, especially those who are both young and perceive their health status as good, are less likely to seek psychological help. Conclusions: The significance and impact of the variables on the probability of individuals seeking psychological help vary for both the entire sample and young people. There may be various restrictions in getting psychological help, and people may resort to informal methods instead of professionals to cope with their existing problems. In developing preventive strategies to safeguard mental health, factors related to young people’s and individuals’ psychological help-seeking status should be prioritized, and more attention should be paid to them.
An Interpretive Ruination Model of the Built Heritage in Inner Areas: The Case Study of the Neighbourhood Granfonte in Leonforte
Authors: Sanzaro Deborah , Trovato Maria Rosa , Circo Chiara
Published: October 2023

In Italy, the current geography of abandonment is defined by the classification of the National Strategy of Inner Areas (NSIA). The support measures envisaged by the NSIA could contribute to reducing the marginality of inner areas and promote the protection, conservation, and enhancement of historic centres. In this perspective, actions on historical buildings should be selected based on a cognitive process aimed at specifying the phenomena that have led to the progressive abandonment of historical centres and a process aimed at identifying the values and dis-values. This research proposes a ruination model aimed at highlighting the risks that the built heritage of the inner areas are exposed to. This model was implemented for the case of the historic neighbourhood of Granfonte in Leonforte. The model was developed based on a qualitative–quantitative approach aimed at detecting the preservation state and built heritage use; analysing the building fabric development; identifying the ruination drivers; building a building units database; defining a ruination pattern of the building units based on a multivariate regression model; and analysing the predicted ruination scenario. The model can support decision-making processes aimed at the selection of actions to protect and preserve heritage built in marginal areas.
Enhancing Transdermal Delivery: Investigating the Impact of Permeation Promoters on Ibuprofen Release and Penetration from Medical Patches—In Vitro Research
Authors: Bednarczyk Paulina , Nowak Anna , Duchnik Wiktoria , Kucharski Łukasz , Ossowicz-Rupniewska Paula
Published: October 2023

This study investigated the impact of various enhancers on permeation through the skin and accumulation in the skin from acrylic pressure-sensitive adhesive-based drug-in-adhesives matrix-type transdermal patches. Eleven patches, each containing a 5% enhancer of permeation, encompassing compounds such as salicylic acid, menthol, urea, glycolic acid, allantoin, oleic acid, Tween 80, linolenic acid, camphor, N-dodecylcaprolactam, and glycerin, were developed. Ibuprofen (IBU) was the model active substance, a widely-used non-steroidal anti-inflammatory drug. The results were compared to patches without enhancers and commercial preparations. The study aimed to assess the effect of enhancers on IBU permeability. The adhesive properties of the patches were characterised, and active substance permeability was tested. The findings revealed that patches with 5% allantoin exhibited the highest IBU permeability, approximately 2.8 times greater than patches without enhancers after 24 h. These patches present a potential alternative to commercial preparations, highlighting the significant impact of enhancers on transdermal drug delivery efficiency.
Exosomes Interactions with the Blood–Brain Barrier: Implications for Cerebral Disorders and Therapeutics
Authors: Osaid Zaynab , Haider Mohamed , Hamoudi Rifat , Harati Rania
Published: October 2023

The Blood–Brain Barrier (BBB) is a selective structural and functional barrier between the circulatory system and the cerebral environment, playing an essential role in maintaining cerebral homeostasis by limiting the passage of harmful molecules. Exosomes, nanovesicles secreted by virtually all cell types into body fluids, have emerged as a major mediator of intercellular communication. Notably, these vesicles can cross the BBB and regulate its physiological functions. However, the precise molecular mechanisms by which exosomes regulate the BBB remain unclear. Recent research studies focused on the effect of exosomes on the BBB, particularly in the context of their involvement in the onset and progression of various cerebral disorders, including solid and metastatic brain tumors, stroke, neurodegenerative, and neuroinflammatory diseases. This review focuses on discussing and summarizing the current knowledge about the role of exosomes in the physiological and pathological modulation of the BBB. A better understanding of this regulation will improve our understanding of the pathogenesis of cerebral diseases and will enable the design of effective treatment strategies.
Contralateral Astrocyte Response to Acute Optic Nerve Damage Is Mitigated by PANX1 Channel Activity
Authors: Wurl Jasmine A. , Mac Nair Caitlin E. , Dietz Joel A. , Shestopalov Valery I. , Nickells Robert W.
Published: October 2023

Glial reactivity is considered a hallmark of damage-induced innate immune responses in the central nervous system. In the visual system, unilateral optic nerve damage elicits dramatic glial reactivity in the retina directly affected by the lesion and a similar, albeit more modest, effect in the contralateral eye. Evaluation of astrocyte changes in a mouse model of optic nerve crush indicates that astrocyte reactivity, as a function of retinal coverage and cellular hypertrophy, occurs within both the experimental and contralateral retinas, although the hypertrophic response of the astrocytes in the contralateral eyes is delayed for at least 24 h. Evaluation of astrocytic reactivity as a function of Gfap expression indicates a similar, muted but significant, response in contralateral eyes. This constrained glial response is completely negated by conditional knock out of Panx1 in both astrocytes and Müller cells. Further studies are required to identify if this is an autocrine or a paracrine suppression of astroglial reactivity.
XPS and HR TEM Elucidation of the Diversity of Titania-Supported Single-Site Ir Catalyst Performance in Spin-Selective Propene Hydrogenation
Authors: Nartova Anna V. , Kvon Ren I. , Kovtunova Larisa M. , Skovpin Ivan V. , Koptyug Igor V. , Bukhtiyarov Valerii I.
Published: October 2023

Immobilized [Ir(COD)Cl]2-Linker/TiO2 catalysts with linkers containing Py, P(Ph)2 and N(CH3)2 functional groups were prepared. The catalysts were tested via propene hydrogenation with parahydrogen in a temperature range from 40 °C to 120 °C which was monitored via NMR. The catalytic behavior of [Ir(COD)Cl]2-Linker/TiO2 is explained on the basis of quantitative and qualitative XPS data analysis performed for the catalysts before and after the reaction at 120 °C. It is shown that the temperature dependence of propene conversion and the enhancement of the NMR signal are explained via a combination of the stabilities of both the linker and immobilized [Ir(COD)Cl]2 complex. It is demonstrated that the N(CH3)2-linker is the most stable at the surface of TiO2 under used reaction conditions. As a result, only this sample shows a rise in the enhancement of the NMR signal in the 100–120 °C temperature range.
Therapeutic Approach Targeting Gut Microbiome in Gastrointestinal Infectious Diseases
Authors: Han Ziying , Min Yiyang , Pang Ke , Wu Dong
Published: October 2023

While emerging evidence highlights the significance of gut microbiome in gastrointestinal infectious diseases, treatments like Fecal Microbiota Transplantation (FMT) and probiotics are gaining popularity, especially for diarrhea patients. However, the specific role of the gut microbiome in different gastrointestinal infectious diseases remains uncertain. There is no consensus on whether gut modulation therapy is universally effective for all such infections. In this comprehensive review, we examine recent developments of the gut microbiome’s involvement in several gastrointestinal infectious diseases, including infection of Helicobacter pylori, Clostridium difficile, Vibrio cholerae, enteric viruses, Salmonella enterica serovar Typhimurium, Pseudomonas aeruginosa Staphylococcus aureus, Candida albicans, and Giardia duodenalis. We have also incorporated information about fungi and engineered bacteria in gastrointestinal infectious diseases, aiming for a more comprehensive overview of the role of the gut microbiome. This review will provide insights into the pathogenic mechanisms of the gut microbiome while exploring the microbiome’s potential in the prevention, diagnosis, prediction, and treatment of gastrointestinal infections.
Combined Inhibition of UBE2C and PLK1 Reduce Cell Proliferation and Arrest Cell Cycle by Affecting ACLY in Pan-Cancer
Authors: Liang Keying , Wang Qian , Qiu Li , Gong Xiaocheng , Chen Zixi , Zhang Haibo , Ding Ke , Liu Yunfei , Wei Jinfen , Lin Shudai , Fu Shuying , Du Hongli
Published: October 2023

Various studies have shown that the cell-cycle-related regulatory proteins UBE2C, PLK1, and BIRC5 promote cell proliferation and migration in different types of cancer. However, there is a lack of in-depth and systematic research on the mechanism of these three as therapeutic targets. In this study, we found a positive correlation between the expression of UBE2C and PLK1/BIRC5 in the Cancer Genome Atlas (TCGA) database, revealing a potential combination therapy candidate for pan-cancer. Quantitative real-time PCR (qRT-PCR), Western blotting (WB), cell phenotype detection, and RNA-seq techniques were used to evidence the effectiveness of the combination candidate. We found that combined interference of UBE2C with PLK1 and UBE2C with BIRC5 affected metabolic pathways by significantly downregulating the mRNA expression of IDH1 and ACLY, which was related to the synthesis of acetyl-CoA. By combining the PLK1 inhibitor volasertib and the ACLY inhibitor bempedoic acid, it showed a higher synergistic inhibition of cell viability and higher synergy scores in seven cell lines, compared with those of other combination treatments. Our study reveals the potential mechanisms through which cell-cycle-related genes regulate metabolism and proposes a potential combined targeted therapy for patients with higher PLK1 and ACLY expression in pan-cancer.
Rod Rotation with Outrigger Is Substantial for Correcting Apical Hypokyphosis in Patients with Adolescent Idiopathic Scoliosis: Novel Outrigger Device for Concave Rod Rotation
Authors: Seki Shoji , Makino Hiroto , Yahara Yasuhito , Kamei Katsuhiko , Futakawa Hayato , Yasuda Taketoshi , Suzuki Kayo , Nakano Masato , Kawaguchi Yoshiharu
Published: October 2023

The apical hypokyphosis of scoliotic patients is thought to lead to decreased lung capacity and cause shortness of breath. Additionally, concave rod curve reduction is a problem in the correction of apical hypokyphosis in posterior spinal fusion surgery in adolescent idiopathic scoliosis (AIS). We investigated the contributions of rod rotation (RR) with an outrigger device, followed by differential rod contouring (DRC) with the outrigger attached to the concave rod, designed to prevent concave rod curve-flattening. We analyzed and compared the results of segmental pedicle screw fixation without the outrigger in 41 AIS patients with thoracic curves (Lenke type I, 25; type II, 16) to those corrected using the outrigger in 36 patients (Lenke type I, 24; type II,12). The changes in the Cobb angle, apical kyphosis of five vertebrae, thoracic kyphosis (TK, T4–12), correction rate, correction angle of apical vertebral rotation, spinal penetration index (SPi), and rib hump index (RHi) before and after surgery were measured, and the contribution of the outrigger was analyzed. The mean scoliosis correction rates without and with the outrigger were 72.1° and 75.6°, respectively (p = 0.03). Kyphosis of the five apical vertebrae and TK were significantly greater in the surgery with the outrigger (p = 0.002). Significantly greater improvements in SPi and RHi were also noted in the surgery with the outrigger (p < 0.05). The use of concave RR and convex DRC with the outrigger appear to be advantageous for correcting apical hypokyphosis, followed by the subsequent formation of TK. As a result, breathing problems are less likely to occur during daily life because of improvements in SPi and RHi.
The Influence of Food Intake and Preload Augmentation on Cardiac Functional Parameters: A Study Using Both Cardiac Magnetic Resonance and Echocardiography
Authors: Visby Lasse , Møgelvang Rasmus , Grund Frederik Fasth , Myhr Katrine Aagaard , Hassager Christian , Vejlstrup Niels , Mattu Raj , Kristensen Charlotte Burup
Published: October 2023

(1) Background: To investigate how food intake and preload augmentation affect the cardiac output (CO) and volumes of the left ventricle (LV) and right ventricle (RV) assessed using cardiac magnetic resonance (CMR) and trans-thoracic echocardiography (TTE). (2) Methods: Eighty-two subjects with (n = 40) and without (n = 42) cardiac disease were assessed using both CMR and TTE immediately before and after a fast infusion of 2 L isotonic saline. Half of the population had a meal during saline infusion (food/fluid), and the other half were kept fasting (fasting/fluid). We analyzed end-diastolic (EDV) and end-systolic (ESV) volumes and feature tracking (FT) using CMR, LV global longitudinal strain (GLS), and RV longitudinal strain (LS) using TTE. (3) Results: CO assessed using CMR increased significantly in both groups, and the increase was significantly higher in the food/fluid group: LV-CO (ΔLV-CO: +2.6 ± 1.3 vs. +0.7 ± 1.0 p < 0.001), followed by increased heart rate (HR) (ΔHR: +12 ± 8 vs. +1 ± 6 p < 0.001). LV and RV achieved increased stroke volume (SV) through different mechanisms. For the LV, through increased contractility, increased LV-EDV, decreased LV-ESV, increased LV-FT, and GLS were observed. For the RV, increased volumes, increased RV-EDV, increased RV-ESV, and at least for the fasting/fluid group, unchanged RV-FT and RV-LS were reported. (4) Conclusions: Preload augmentation and food intake have a significant impact on hemodynamic and cardiac functional parameters. This advocates for standardized recommendations regarding oral intake of fluid and food before cardiac assessment, for example, TTE, CMR, and right heart catheterization. We also demonstrate different approaches for the LV and RV to increase SV: for the LV by increased contractility, and for the RV by volume expansion.
Factors Affecting the Length of Stay in the Intensive Care Unit among Adults in Saudi Arabia: A Cross-Sectional Study
Authors: Alharbi Khulud K. , Arbaein Turky J. , Alzhrani Abdulrhman A. , Alzahrani Ali M. , Monshi Sarah S. , Alotaibi Adel Fahad M. , Aljasser Areej I. , Alruhaimi Khalil Thawahi , Alotaibi Satam Dhafallah K. , Alsultan Ali K. , Arafat Mohammed S. , Aldhabib Abdulrahman , Abd-Ellatif Eman E.
Published: October 2023

This study aimed to assess patient-related factors associated with the LOS among adults admitted to the ICU in Saudi Arabia. The Ministry of Health provided a cross-sectional dataset for 2021, which served as the data source for this study. The data included data on adults admitted to different ICUs at various hospitals. The number of days spent in the ICU was the outcome variable of interest. The potential predictors were age, sex, and nationality, as well as clinical data from the time of admission. Descriptive statistics and bivariate analysis were used to analyse the association between the predictors and the ICU LOS and characterize how they were distributed. We used negative binomial regression to examine the relationship between the study predictors and the ICU LOS. A total of 42,884 individuals were included in this study, of whom 25,520 were men and 17,362 were women. The overall median ICU LOS was three days. This study showed that the ICU LOS was highly influenced by the patient’s age, sex, nationality, source of admission, and clinical history. Several predictors that affect how long adults stay in the ICU in Saudi Arabian hospitals were identified in this study. These factors can be attributed to variances in health care delivery systems, patient demographics, and cultural considerations. To allocate resources efficiently, enhance patient outcomes, and create focused treatments to reduce ICU LOS, it is essential to comprehend these elements.
Contemporary Perspectives in Pathophysiology of Facial Nerve Damage in Oto-Neurological and Skull Base Surgical Procedures: A Narrative Review
Authors: Franz Leonardo , Marioni Gino , Mazzoni Antonio , de Filippis Cosimo , Zanoletti Elisabetta
Published: October 2023

During the last decades, neuro-otological surgery has progressively reduced functional morbidity, including facial nerve damage. However, the occurrence of this sequela may significantly impact on patients’ quality of life. The aim of this narrative review is to provide an update on the patho-physiological and clinical issues related to facial nerve damage in oto-neurological and skull base surgery, in the light of a comprehensive therapeutic and rehabilitative approach to iatrogenic disfunctions. The narrative review is based on a search in the PubMed, Scopus, and Web of Science databases. In this surgical setting, the onset of intraoperative facial nerve damage is related to various aspects, mainly concerning the anatomical relationship between tumor and nerve, the trajectory of the surgical corridor, and the boundaries of the resection margins. Mechanisms related to stretching, compression, devascularization, and heating may play a role in determining intraoperative facial nerve damage and provide the patho-physiological basis for possible nerve regeneration disorders. Most of the studies included in this review, dealing with the pathophysiology of surgical facial nerve injury, were preclinical. Future research should focus on the association between intraoperative trauma mechanisms and their clinical correlates in surgical practice. Further investigations should also be conducted to collect and record intraoperative data on nerve damage mechanisms, as well as the reports from neuro-monitoring systems.
Histological Assessment of the Bile Duct before Liver Transplantation: Does the Bile Duct Injury Score Predict Biliary Strictures?
Authors: Ly Mark , Lau Ngee-Soon , McKenzie Catriona , Kench James G. , Seyfi Doruk , Majumdar Avik , Liu Ken , McCaughan Geoffrey , Crawford Michael , Pulitano Carlo
Published: October 2023

Introduction: Histological injury to the biliary tree during organ preservation leads to biliary strictures after liver transplantation. The Bile Duct Injury (BDI) score was developed to assess histological injury and identify the grafts most likely to develop biliary strictures. The BDI score evaluates the bile duct mural stroma, peribiliary vascular plexus (PVP) and deep peribiliary glands (DPGs), which were correlated with post-transplant biliary strictures. However, the BDI score has not been externally validated. The aim of this study was to verify whether the BDI score could predict biliary strictures at our transplant centre. Methods: Brain-dead donor liver grafts transplanted at a single institution from March 2015 to June 2016 were included in this analysis. Bile duct biopsies were collected immediately before transplantation and assessed for bile duct injury by two blinded pathologists. The primary outcome was the development of clinically significant biliary strictures within 24 months post-transplant. Results: Fifty-seven grafts were included in the study which included 16 biliary strictures (28%). Using the BDI score, mural stromal, PVP and DPG injury did not correlate with biliary strictures including Non-Anastomotic Strictures. Severe inflammation (>50 leucocytes per HPF) was the only histological feature inversely correlated with the primary outcome (absent in the biliary stricture group vs. 41% in the no-stricture group, p = 0.001). Conclusions: The current study highlights limitations of the histological assessment of bile duct injury. Although all grafts had bile duct injury, only inflammation was associated with biliary strictures. The BDI score was unable to predict post-transplant biliary strictures in our patient population.