bluuebunny/crossref_metadata_2025 huggingface dataset:
Injectable Hyaluronan-Based Thermoresponsive Hydrogels for Dermatological Applications
Authors: Gou Si , Porcello Alexandre , Allémann Eric , Salomon Denis , Micheels Patrick , Jordan Olivier , Kalia Yogeshvar N.
Published: June 2023

Most marketed HA-based dermal fillers use chemical cross-linking to improve mechanical properties and extend their lifetime in vivo; however, stiffer products with higher elasticity require an increased extrusion force for injection in clinical practice. To balance longevity and injectability, we propose a thermosensitive dermal filler, injectable as a low viscosity fluid that undergoes gelation in situ upon injection. To this end, HA was conjugated via a linker to poly(N-isopropylacrylamide) (pNIPAM), a thermosensitive polymer using “green chemistry”, with water as the solvent. HA-L-pNIPAM hydrogels showed a comparatively low viscosity (G′ was 105.1 and 233 for Candidate1 and Belotero Volume®, respectively) at room temperature and spontaneously formed a stiffer gel with submicron structure at body temperature. Hydrogel formulations exhibited superior resistance against enzymatic and oxidative degradation and could be administered using a comparatively lower injection force (49 N and >100 N for Candidate 1 and Belotero Volume®, respectively) with a 32G needle. Formulations were biocompatible (viability of L929 mouse fibroblasts was >100% and ~85% for HA-L-pNIPAM hydrogel aqueous extract and their degradation product, respectively), and offered an extended residence time (up to 72 h) at the injection site. This property could potentially be exploited to develop sustained release drug delivery systems for the management of dermatologic and systemic disorders.
Range-Gated LIDAR Utilizing a LiNbO3 (LN) Crystal as an Optical Switch
Authors: Luan Chenglong , Li Yingchun , Guo Huichao , Sun Houpeng
Published: June 2023

In this paper, a range-gated LIDAR system utilizing an LN crystal as the electro-optical switch and a SCMOS (scientific complementary metal oxide semiconductor) imaging device is designed. To achieve range-gated operations, we utilize two polarizers and an LN (LiNbO3) crystal to form an electro-optical switch. The optical switch is realized by applying a pulse voltage at both ends of the crystal due to the crystal’s conoscopic interference effect and electro-optical effect. The advantage of this system is that low-bandwidth detectors, such as a CMOS and a CCD (charge-coupled device), can be used to replace conventional high-bandwidth detectors, such as an ICCD (intensified charge-coupled device), and it displays better imaging performance under specific conditions at the same time. However, after using an electro-optical crystal as an optical switch, a new inhomogeneity error will be introduced due to the conoscopic interference effect of the electro-optical crystal, resulting in a range error for the LIDAR system. To reduce the influence of inhomogeneity error on the system, this paper analyzes the sources of inhomogeneity error caused by the electro-optical crystal and calculates the crystal’s inhomogeneity mathematical expression. A compensation method is proposed based on the above inhomogeneity mathematical expression. An experimental LIDAR system is constructed in this paper to verify the validity of the compensation method. The experimental results of the range-gated LIDAR system show that in a specific field of view (2.6 mrad), the LIDAR system has good imaging performance; its ranging standard deviation is 3.86 cm and further decreases to 2.86 cm after compensation, which verifies the accuracy of the compensation method.
Genetic Dissection of Extreme Seed-Flooding Tolerance in a Wild Soybean PI342618B by Linkage Mapping and Candidate Gene Analysis
Authors: Yu Zhe-Ping , Lv Wen-Huan , Sharmin Ripa Akter , Kong Jie-Jie , Zhao Tuan-Jie
Published: June 2023

Seed-flooding stress is one of major abiotic constraints that adversely affects soybean production worldwide. Identifying tolerant germplasms and revealing the genetic basis of seed-flooding tolerance are imperative goals for soybean breeding. In the present study, high-density linkage maps of two inter-specific recombinant inbred line (RIL) populations, named NJIRNP and NJIR4P, were utilized to identify major quantitative trait loci (QTLs) for seed-flooding tolerance using three parameters viz., germination rate (GR), normal seedling rate (NSR), and electrical conductivity (EC). A total of 25 and 18 QTLs were detected by composite interval mapping (CIM) and mixed-model-based composite interval mapping (MCIM), respectively, and 12 common QTLs were identified through both methods. All favorable alleles for the tolerance are notably from the wild soybean parent. Moreover, four digenic epistatic QTL pairs were identified, and three of them showed no main effects. In addition, the pigmented soybean genotypes exhibited high seed-flooding tolerance compared with yellow seed coat genotypes in both populations. Moreover, out of five identified QTLs, one major region containing multiple QTLs associated with all three traits was identified on Chromosome 8, and most of the QTLs within this hotspot were major loci (R2 > 10) and detectable in both populations and multiple environments. Based on the gene expression and functional annotation information, 10 candidate genes from QTL “hotspot 8-2” were screened for further analysis. Furthermore, the results of qRT-PCR and sequence analysis revealed that only one gene, GmDREB2 (Glyma.08G137600), was significantly induced under flooding stress and displayed a TTC tribasic insertion mutation of the nucleotide sequence in the tolerant wild parent (PI342618B). GmDREB2 encodes an ERF transcription factor, and the subcellular localization analysis using green fluorescent protein (GFP) revealed that GmDREB2 protein was localized in the nucleus and plasma membrane. Furthermore, overexpression of GmDREB2 significantly promoted the growth of soybean hairy roots, which might indicate its critical role in seed-flooding stress. Thus, GmDREB2 was considered as the most possible candidate gene for seed-flooding tolerance.
Kalanchoe sp. Extracts—Phytochemistry, Cytotoxic, and Antimicrobial Activities
Authors: Stefanowicz-Hajduk Justyna , Hering Anna , Kowalczyk Mariusz , Hałasa Rafał , Gucwa Magdalena , Ochocka J. Renata
Published: June 2023

Kalanchoe species are succulents occurring in tropical regions. They have many biological and pharmacological properties. In this study, the cytotoxic and antimicrobial activities of water and dichloromethane Kalanchoe fractions obtained from ethanol extracts of three Kalanchoe species—K. daigremontiana, K. pinnata, and K. blossfeldiana were estimated. The cytotoxic effect was assessed on human cancer cell lines—ovarian SKOV-3, cervical HeLa, breast MCF-7, and melanoma A375—by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The antimicrobial activity was estimated on selected Gram-positive and Gram-negative bacteria strains and on Candida albicans. The phytochemical analysis of selected Kalanchoe extracts was conducted by LC-QTOF-MS. The obtained results showed that the water fraction of K. blossfeldiana was active both on the tested cancer cells (IC50 values were 28.28 ± 2.76 and 32.51 ± 0.69 µg/mL on HeLa and SKOV-3, respectively) and bacteria strains (MIC values were 16 and 32 µg/mL on S. epidermidis and S. aureus, respectively). The water fraction of K. pinnata also had a significant effect on S. epidermidis and S. aureus, with MIC values of 32 and 64 µg/mL, respectively. The water fraction of K. blossfeldiana triggered a decrease in mitochondrial membrane potential (MMP) and induced cell cycle arrest in the G2/M phase in the SKOV-3 and HeLa cells. This fraction did not significantly increase cellular oxidative stress level. The DPPH and ABTS assays revealed that the water fraction of K. blossfeldiana had a strong antioxidant effect (IC50 was 9.44 ± 0.06 and 3.17 ± 0.1 µg/mL, respectively). The phytochemical analysis of the extracts of K. blossfeldiana and K. pinnata revealed the presence of at least 218 main components. The most frequently occurring were flavonol glycosides (31 metabolites), phenylpropanoids (13 metabolites), gallic acid derivatives (13 compounds), benzoic acid derived compounds (14 metabolites), and acyclic alcohol glycosides (16 compounds). In addition, proanthocyanidins were detected mainly in K. blossfeldiana. The study indicates that the water fraction of K. blossfeldiana has significant biological potential and can be further investigated towards anticancer and antimicrobial application.
Host Factors Genes BcCLC1 and BcCLC2 Confer Turnip Mosaic Virus Resistance in Non-Heading Chinese Cabbage (Brassica campestris ssp. chinensis)
Authors: Yuan Mengguo , Lyu Shanwu , Wang Yaolong , E Liu , Liu Tongkun , Hou Xilin , Li Ying , Zhang Changwei
Published: June 2023

Clathrin is an evolutionarily highly conserved evolutionary protein consisting of clathrin light chains (CLC) and clathrin heavy chains (CHC), and these form its basic structure. Clathrin is an important host factor in the process of viral infection. In this study, we cloned the BcCLC1 gene and the BcCLC2 gene from the ‘49CX’ variety of non-heading Chinese cabbage (NHCC, Brassica campestris L. ssp. chinensis Makino) and verified their functions. The results showed that BcCLC1 was mainly localized in the cytomembrane and cytoplasm, and only a small amount entered the nucleus. BcCLC2 encoded a protein comprising 265 amino acids that were distributed in the cytomembrane, nucleus, and cytoplasm. A BiFC assay and yeast two-hybrid (Y2H) analysis showed that BcCLCs (BcCLC1 and BcCLC2) could interact with several TuMV proteins. We further investigated the mechanism of BcCLCs in regulating TuMV virus infections in NHCC, and observed that BcCLCs gene silencing inhibited TuMV infections and overexpression of BcCLCs in Arabidopsis promoted TuMV infections in NHCC. Finally, mutants of Arabidopsis homologs of BcCLCs were also screened and subjected to TuMV inoculation tests. In conclusion, we speculate that BcCLCs confer Turnip mosaic virus (TuMV) resistance in NHCC by interacting with TuMV proteins to promote the intracellular transport of the virus.
Antioxidant, Anti-Inflammatory and Anti-Proliferative Properties of Stachys circinata on HepG2 and MCF7 Cells
Authors: Slimani Wassila , Maioli Margherita , Cruciani Sara , Zerizer Sakina , Santaniello Sara , Kabouche Zahia , Coradduzza Donatella , Chessa Mario , Fancello Silvia , Migheli Rossana , Serra Pier Andrea , D’hallewin Guy
Published: June 2023

According to the WHO, the overall age-standardized cancer rate keeps declining, and the number of cases diagnosed each year increases, remaining among the leading causes of death in 91 out of 172 recorded countries. In this context, novel cancer prediction and therapeutic protocols are compulsory. The effect of a Stachys circinata L’Hér dichloromethane extract (ScDME) on cell redox homeostasis and tumor proliferation was investigated. HepG2 cell feedback mechanisms to oxidative stress exposure were evaluated by determining catalase (CAT) and reduced glutathione (GSH), following the supply with ScDME (0.0–5.7 µg/µL). Cytotoxicity of ScDME against the human umbilical vein endothelial cell (HUVEC) and two human cancer cell lines (breast: MCF7; liver: HepG2) was evaluated by the MTT assay. H2O2-stressed HepG2 cells supplied with the S. circinata extracts exhibited significantly increased CAT and GSH activity as compared to unsupplied ones. The anti-inflammatory activity of the extracts was evaluated by real time-qPCR on IL-1, IL-6 and TNF-α expression. As a result, this research points out that S. circinata dichloromethane extract owns anti-inflammatory and anti-proliferative properties against MCF7 and HepG2 cells and activates CAT and GSH of the HepG2 cells’ antioxidant enzyme system.
Response and Tolerance of Macleaya cordata to Excess Zinc Based on Transcriptome and Proteome Patterns
Authors: Zhang Hongxiao , Hu Linfeng , Du Xinlong , Shah Assar Ali , Ahmad Baseer , Yang Liming , Mu Zhiying
Published: June 2023

Macleaya cordata is a dominant plant of mine tailings and a zinc (Zn) accumulator with high Zn tolerance. In this study, M. cordata seedlings cultured in Hoagland solution were treated with 200 μmol·L−1 of Zn for 1 day or 7 days, and then, their leaves were taken for a comparative analysis of the transcriptomes and proteomes between the leaves of the control and Zn treatments. Differentially expressed genes included those that were iron (Fe)-deficiency-induced, such as vacuolar iron transporter VIT, ABC transporter ABCI17 and ferric reduction oxidase FRO. Those genes were significantly upregulated by Zn and could be responsible for Zn transport in the leaves of M. cordata. Differentially expressed proteins, such as chlorophyll a/b-binding proteins, ATP-dependent protease, and vacuolar-type ATPase located on the tonoplast, were significantly upregulated by Zn and, thus, could be important in chlorophyll biosynthesis and cytoplasm pH stabilization. Moreover, the changes in Zn accumulation, the production of hydrogen peroxide, and the numbers of mesophyll cells in the leaves of M. cordata were consistent with the expression of the genes and proteins. Thus, the proteins involved in the homeostasis of Zn and Fe are hypothesized to be the keys to the tolerance and accumulation of Zn in M. cordata. Such mechanisms in M. cordata can suggest novel approaches to genetically engineering and biofortifying crops.
Water Metabolism of Lonicera japonica and Parthenocissus quinquefolia in Response to Heterogeneous Simulated Rock Outcrop Habitats
Authors: Zhao Xiaopan , Wu Yanyou , Xing Deke , Li Haitao , Zhang Furong
Published: June 2023

The karst carbon sink caused by rock outcrops results in enrichment of the bicarbonate in soil, affecting the physiological process of plants in an all-round way. Water is the basis of plant growth and metabolic activities. In heterogeneous rock outcrop habitats, the impact of bicarbonate enrichment on the intracellular water metabolism of plant leaf is still unclear, which needs to be revealed. In this paper, the Lonicera japonica and Parthenocissus quinquefolia plants were selected as experimental materials, and electrophysiological indices were used to study their water holding, transfer and use efficiency under three simulated rock outcrop habitats, i.e., rock/soil ratio as 1, 1/4 and 0. By synchronously determining and analyzing the leaf water content, photosynthetic and chlorophyll fluorescence parameters, the response characteristics of water metabolism within leaf cells to the heterogeneous rock outcrop habitats were revealed. The results showed that the soil bicarbonate content in rock outcrop habitats increased with increasing rock/soil ratio. Under the treatment of a higher concentration of bicarbonate, the leaf intra- and intercellular water acquisition and transfer efficiency as well as the photosynthetic utilization capacity of P. quinquefolia decreased, the leaf water content was lower, and those plants had low bicarbonate utilization efficiency, which greatly weakened their drought resistance. However, the Lonicera japonica had a high bicarbonate use capacity when facing the enrichment of bicarbonate within cells, the above-mentioned capacity could significantly improve the water status of the leaves, and the water content and intracellular water-holding capacity of plant leaves in large rock outcrop habitats were significantly better than in non-rock outcrop habitats. In addition, the higher intracellular water-holding capacity was likely to maintain the stability of the intra- and intercellular water environment, thus ensuring the full development of its photosynthetic metabolic capacity, and the stable intracellular water-use efficiency also made itself more vigorous under karstic drought. Taken together, the results suggested that the water metabolic traits of Lonicera japonica made it more adaptable to karst environments.
Characterization of the PIN Auxin Efflux Carrier Gene Family and Its Expression during Zygotic Embryogenesis in Persea americana
Authors: Monroy-González Zurisadai , Uc-Chuc Miguel A. , Quintana-Escobar Ana O. , Duarte-Aké Fátima , Loyola-Vargas Víctor M.
Published: June 2023

Auxins are responsible for a large part of the plant development process. To exert their action, they must move throughout the plant and from cell to cell, which is why plants have developed complex transport systems for indole-3-acetic acid (IAA). These transporters involve proteins that transport IAA into cells, transporters that move IAA to or from different organelles, mainly the endoplasmic reticulum, and transporters that move IAA out of the cell. This research determined that Persea americana has 12 PIN transporters in its genome. The twelve transporters are expressed during different stages of development in P. americana zygotic embryos. Using different bioinformatics tools, we determined the type of transporter of each of the P. americana PIN proteins and their structure and possible location in the cell. We also predict the potential phosphorylation sites for each of the twelve-PIN proteins. The data show the presence of highly conserved sites for phosphorylation and those sites involved in the interaction with the IAA.
Sorafenib-Based Drug Delivery Systems: Applications and Perspectives
Authors: Wang Lingyun , Chen Meihuan , Ran Xueguang , Tang Hao , Cao Derong
Published: June 2023

As a Food and Drug Administration (FDA)-approved molecular-targeted chemotherapeutic drug, sorafenib (SF) can inhibit angiogenesis and tumor cell proliferation, leading to improved patient overall survival of hepatocellular carcinoma (HCC). In addition, SF is an oral multikinase inhibitor as a single-agent therapy in renal cell carcinoma. However, the poor aqueous solubility, low bioavailability, unfavorable pharmacokinetic properties and undesirable side effects (anorexia, gastrointestinal bleeding, and severe skin toxicity, etc.) seriously limit its clinical application. To overcome these drawbacks, the entrapment of SF into nanocarriers by nanoformulations is an effective strategy, which delivers SF in a target tumor with decreased adverse effects and improved treatment efficacy. In this review, significant advances and design strategies of SF nanodelivery systems from 2012 to 2023 are summarized. The review is organized by type of carriers including natural biomacromolecule (lipid, chitosan, cyclodextrin, etc.); synthetic polymer (poly(lactic-co-glycolic acid), polyethyleneimine, brush copolymer, etc.); mesoporous silica; gold nanoparticles; and others. Co-delivery of SF and other active agents (glypican-3, hyaluronic acid, apolipoprotein peptide, folate, and superparamagnetic iron oxide nanoparticles) for targeted SF nanosystems and synergistic drug combinations are also highlighted. All these studies showed promising results for targeted treatment of HCC and other cancers by SF-based nanomedicines. The outlook, challenges and future opportunities for the development of SF-based drug delivery are presented.
Influence of Vinyl Acetate Content and Melt Flow Index of Ethylene-Vinyl Acetate Copolymer on Physico-Mechanical and Physico-Chemical Properties of Highly Filled Biocomposites
Authors: Shelenkov Pavel G. , Pantyukhov Petr V. , Poletto Matheus , Popov Anatoly A.
Published: June 2023

Highly filled biocomposites may be used as biodegradable masterbatches that manufacturers add to traditional polymers for making plastic goods more biodegradable. Biocomposites based on various trademarks of ethylene-vinyl acetate copolymer (EVA) and natural vegetable fillers (wood flour and microcrystalline cellulose) were studied. The EVA trademarks differed both in terms of the melt flow index and in the content of vinyl acetate groups. The composites were created as superconcentrates (or masterbatches) for the production of biodegradable materials based on vegetable fillers with polyolefin matrices. The filler content in biocomposites was 50, 60, 70 wt.%. The influence of the content of vinyl acetate in the copolymer and its melt flow index on the physico-mechanical and rheological properties of highly filled biocomposites was evaluated. As a result, one EVA trademark with high molecular weight that has a high content of VA was chosen because of its optimal parameters for creating highly filled composites with natural fillers.
Toughening Weak Polyampholyte Hydrogels with Weak Chain Entanglements via a Secondary Equilibrium Approach
Authors: Liu Tao , Chen Wenjun , Li Kai , Long Shijun , Li Xuefeng , Huang Yiwan
Published: June 2023

Polyampholyte (PA) hydrogels are randomly copolymerized from anionic and cationic monomers, showing good mechanical properties owing to the existence of numerous ionic bonds in the networks. However, relatively tough PA gels can be synthesized successfully only at high monomer concentrations (CM), where relatively strong chain entanglements exist to stabilize the primary supramolecular networks. This study aims to toughen weak PA gels with relatively weak primary topological entanglements (at relatively low CM) via a secondary equilibrium approach. According to this approach, an as-prepared PA gel is first dialyzed in a FeCl3 solution to reach a swelling equilibrium and then dialyzed in sufficient deionized water to remove excess free ions to achieve a new equilibrium, resulting in the modified PA gels. It is proved that the modified PA gels are eventually constructed by both ionic and metal coordination bonds, which could synergistically enhance the chain interactions and enable the network toughening. Systematic studies indicate that both CM and FeCl3 concentration (\({{C}_{\text{FeCl}_{3}}}\)) influence the enhancement effectiveness of the modified PA gels, although all the gels could be dramatically enhanced. The mechanical properties of the modified PA gel could be optimized at CM = 2.0 M and \({{C}_{\text{FeCl}_{3}}}\) = 0.3 M, where the Young’s modulus, tensile fracture strength, and work of tension are improved by 1800%, 600%, and 820%, respectively, comparing to these of the original PA gel. By selecting a different PA gel system and diverse metal ions (i.e., Al3+, Mg2+, Ca2+), we further prove that the proposed approach is generally appliable. A theoretical model is used to understand the toughening mechanism. This work well extends the simple yet general approach for the toughening of weak PA gels with relatively weak chain entanglements.
Determination of the Attenuation Coefficients of Epoxy Resin with Carbopol Polymer as a Breast Phantom Material at Low Photon Energy Range
Authors: Marashdeh Mohammad , Abdulkarim Muthanna
Published: June 2023

Six different composites of epoxy resin and Carbopol 974p polymer were prepared based on Carbopol 974p polymer concentrations of 0%, 5%, 10%, 15%, 20%, and 25%. The linear and mass attenuation coefficients, Half Value Layer (HVL), and mean free path (MFP) of these composites were determined using single-beam photon transmission in the energy range between 16.65 keV and 25.21 keV. This was carried out by determining the attenuation of ka1 X-ray fluorescent (XRF) photons from niobium, molybdenum, palladium, silver, and tin targets. The results were compared with theoretical values of three types of breast material (Breast 1, Breast 2, Breast 3) and Perspex, which was calculated using a XCOM computer program. The results show that there were no significant differences in the attenuation coefficient values after the consequent Carbopol additions. Moreover, it was found that the mass attenuation coefficients of all tested composites were close to those of Perspex and the values for Breast 3. The HVL and MFP results showed that the E25 sample is closer to the results of the Perspex material with differences of (0.53–1.15%) and (0.51–1.20%), respectively. In addition, the densities of the fabricated samples were in the range of 1.102–1.170 g/cm3, which is in the range of human breast density. A computed tomography (CT) scanner was used to investigate the CT number values for the fabricated samples. The CT numbers of all samples were in the range of human breast tissue (24.53–40.28 HU). Based on these findings, the fabricated epoxy–Carbopol polymer is a good candidate for use as a breast phantom material.
Insulin-Loaded Soybean Trypsin Inhibitor-Chitosan Nanoparticles: Preparation, Characterization, and Protective Effect Evaluation
Authors: Zhang Yihao , Liu Ruijia , Feng Qixu , Li He , Li You , Liu Xinqi
Published: June 2023

The aim of this work was to prepare insulin-loaded nanoparticles using soybean trypsin inhibitor (STI) and chitosan (CS) as a potential coating. The nanoparticles were prepared by complex coacervation, and characterized for their particle size, polydispersity index (PDI), and encapsulation efficiency. In addition, the insulin release and enzymatic degradation of nanoparticles in simulated gastric fluid (SGF) and simulated intestinal fluid (SIF) were evaluated. The results showed that the optimal conditions for preparing insulin-loaded soybean trypsin inhibitor-chitosan (INs-STI-CS) nanoparticles were as follows: CS concentration of 2.0 mg/mL, STI concentration of 1.0 mg/mL, and pH 6.0. The INs-STI-CS nanoparticles prepared at this condition had a high insulin encapsulation efficiency of 85.07%, the particle diameter size was 350 ± 5 nm, and the PDI value was 0.13. The results of the in vitro evaluation of simulated gastrointestinal digestion showed that the prepared nanoparticles could improve the stability of insulin in the gastrointestinal tract. Compared with free insulin, the insulin loaded in INs-STI-CS nanoparticles was retained at 27.71% after 10 h of digestion in the intestinal tract, while free insulin was completely digested. These findings will provide a theoretical basis for improving the stability of oral insulin in the gastrointestinal tract.
Collagen Scaffolds Laden with Human Periodontal Ligament Fibroblasts Promote Periodontal Regeneration in SD Rat Model
Authors: Chang Yi-Tao , Lai Chuan-Ching , Lin Dan-Jae
Published: June 2023

Periodontitis, a chronic inflammatory disease caused by microbial communities carrying pathogens, leads to the loss of tooth-supporting tissues and is a significant contributor to tooth loss. This study aims to develop a novel injectable cell-laden hydrogel consisted of collagen (COL), riboflavin, and a dental light-emitting diode (LED) photo-cross-linking process for periodontal regeneration. Utilizing α-SMA and ALP immunofluorescence markers, we confirmed the differentiation of human periodontal ligament fibroblasts (HPLFs) into myofibroblasts and preosteoblasts within collagen scaffolds in vitro. Twenty-four rats with three-wall artificial periodontal defects were divided into four groups, Blank, COL_LED, COL_HPLF, and COL_HPLF_LED, and histomorphometrically assessed after 6 weeks. Notably, the COL_HPLF_LED group showed less relative epithelial downgrowth (p < 0.01 for Blank, p < 0.05 for COL_LED and COL_HPLF), and the relative residual bone defect was significantly reduced in the COL_HPLF_LED group compared to the Blank and the COL_LED group (p < 0.05). The results indicated that LED photo-cross-linking collagen scaffolds possess sufficient strength to withstand the forces of surgical process and biting, providing support for HPLF cells embedded within them. The secretion of cells is suggested to promote the repair of adjacent tissues, including well-oriented periodontal ligament and alveolar bone regeneration. The approach developed in this study demonstrates clinical feasibility and holds promise for achieving both functional and structural regeneration of periodontal defects.
Kalikhasang Balaan: Elements of a Youth Specific Ecotheology in the Philippines
Authors: Baring Rito , Molino Jeramie
Published: June 2023

Scientific consensus points to human activity as the primary cause of global warming triggering climate change. Mitigations include technology-assisted interventions and education of human agents, such as changing the human mindset and behavior, to avoid impending, irreversible environmental damage, among others. Among the competent sectors, religious communities can boost formative human interventions through religious ideas. The present study content analyzes the conceptual elements of “Kalikhasang Balaan” as a youth-specific ecotheological view drawn from a unidimensional Filipino measure on Christian Environmentalism. Our analysis is deductive in approach, drawing theological aspects of a peculiar youth ecotheology driven by the notion of “Kalikhasang Balaan”, framed within creation theology and Christian stewardship driven by environmental ethics. We discuss the unique attributes of this Filipino notion in the light of local Philippine church and government responses to understand how the youth ecotheological voice may correspond to institutional views.
Traditional Conflict Management: How Early Interpreters Address Paul’s Reference to Those Baptized for the Dead (1 Corinthians 15:29)
Authors: Stark J. David
Published: June 2023

Modern scholars often understand 1 Cor 15:29 as a clear reference to the baptism of living individuals as proxies for the departed. Yet before or at the time of 1 Corinthians, there appears to be no evidence for this practice or a similar one. A reasonable explanation for its emergence, therefore, is that the tradition derives from 1 Cor 15:29, rather than giving rise to it. Consequently, 1 Cor 15:29 supplies a unique opportunity to see how Paul’s earliest interpreters navigated the conflict between the emergent proxy baptism tradition and others they had inherited. Responses varied from acceptance (Marcion) to tolerance (Ambrosiaster) to rejection (Tertullian, Didymus, Epiphanius, Chrysostom, Eznik) of proxy baptism as being what 1 Cor 15:29 describes. Adopters of proxy baptism found support in Paul for breaking with prior tradition and interpreted 1 Cor 15:29 as a basis for creating a new tradition that fit a distinctive need in their community. By contrast, those who tolerate or reject the proxy baptism interpretation do so by considering both 1 Cor 15:29 and Paul himself as more thoroughly situated within existing prior traditions that rule out proxy baptism. These different responses illustrate the complex interplay between Paul and the conflicting traditions through which his letters have been and continue to be received. These responses also surface key features of the interplay between conflict and tradition, whether that conflict occurs within explicitly religious spheres or not.
The Calibration Method of Multi-Channel Spatially Varying Amplitude-Phase Inconsistency Errors in Airborne Array TomoSAR
Authors: Wang Dawei , Zhang Fubo , Chen Longyong , Li Zhenhua , Yang Ling
Published: June 2023

Airborne array tomographic synthetic aperture radar (TomoSAR) can acquire three-dimensional (3D) information of the observed scene in a single pass. In the process of airborne array TomoSAR data imaging, due to the disturbance of factors such as inconsistent antenna patterns and baseline errors, there are spatially varying amplitude-phase inconsistency errors in the multi-channel Single-Look-Complex (SLC) images. The existence of the errors degrades the quality of the 3D imaging results, which suffer from positioning errors, stray points, and spurious targets. In this paper, a new calibration method based on multiple prominent points is proposed to calibrate the errors of amplitude-phase inconsistency. Firstly, the prominent points are selected from the multi-channel SLC data. Then, the subspace decomposition method and maximum interference spectrum method are used to extract the multi-channel amplitude-phase inconsistency information at each point. The last step is to fit the varying curve and to compensate for the errors. The performance of the method is verified using actual data. The experimental results show that compared with the traditional fixed amplitude-phase inconsistency calibration method, the proposed method can effectively calibrate spatially varying amplitude-phase inconsistency errors, thus improving on the accuracy of 3D reconstruction results for large-scale scenes.
Do Ecological Restoration Projects Undermine Economic Performance? A Spatially Explicit Empirical Study in Loess Plateau, China
Authors: Li Shicheng , Xie Jinqian , Paudel Basanta
Published: June 2023

Exploring the complex relationship between ecological restoration and economic development is valuable for decision makers to formulate policy for sustainable development. The large-scale environmental restoration program—Grain for Green—was mainly implemented in the Loess Plateau of China to improve the soil retention service. However, whether this world-famous program affects local economic development has not been fully explored. In this study, using the Integrated Valuation of Ecosystem Services and Tradeoffs (InVEST) model and spatializing the gross domestic product (GDP) based on the remotely sensed nightlight data, we explored the tradeoff between environment (i.e., soil retention service) and economy (i.e., GDP) for the Loess Plateau in a spatially explicit way. We found that the soil retention service increased prominently over the past 40 years, especially after implementing the Grain for Green project. Meanwhile, the GDP increased about nine-fold over the past four decades from 4.52 to 40.29 × 107 USD. A win–win situation of soil retention and economic development was achieved in the Loess Plateau of China, particularly in the loess gully and loess hilly gully regions of the Loess Plateau. The win–win situation of soil retention and economic development was as a result of the Grain for Green program, the optimization of industrial structure, and the increase in non-agriculture employment. Compared with previous studies, more spatial information was available for the Loess Plateau in this study, which is more valuable to policymakers.
DInSAR Data Reveal an Intriguing Contemporaneous Onset of Deep Deflation below Vesuvio and the Ongoing Campi Flegrei Uplift
Authors: Amoruso Antonella , Crescentini Luca
Published: June 2023

Campi Flegrei and Vesuvio volcanoes are only about 25 km apart, located on opposite sides of the densely inhabited area of Naples (Italy). Since neighbouring volcanoes may influence each other’s activity, it is of great interest to identify signs of any mutual interaction between Campi Flegrei and Vesuvio, or at least note coincidences in their recent deformation dynamics. After a large uplift, Campi Flegrei was generally subsiding from 1985 to 2001, while it has been uplifting—probably driven by deep magma inflation—at an accelerating rate since then. Here, we analysed the ground displacement in the whole Vesuvian area and its surroundings around the early 2000s using 1993–2010 ERS/ENVISAT ascending- and descending-orbit line-of-sight displacements obtained through the Small BAseline Subset Differential Synthetic Aperture Radar Interferometry technique. Although ground deformation is slow—a few millimetres per year—Empirical Orthogonal Function analysis shows a sudden trend change around 2001. Pre-2001 velocity maps confirm previously published results: subsidence mainly occurred inside the caldera rim—probably because of the sliding and compaction of young incoherent materials—and in a few spots around 10 km from the summital crater; eastward displacement occurred in a lobe east of Vesuvio, and westward displacement occurred in a lobe west of Vesuvio, as in the case of the spreading of the volcanic edifice and/or extensional tectonics. We attribute the subsidence spots to the previous high local number of new buildings per year. Post-2002 velocity maps provide evidence of a very different scenario: general subsidence in the whole Vesuvian area, westward displacement in a lobe east of Vesuvio, and eastward displacement in a lobe west of Vesuvio. This last arrangement of the ground displacement field is made even clearer by subtracting the post-2002 velocity from the pre-2001 value. The results of our analyses are consistent with the deflation of a deep pressurised source. Additionally, Vesuvio’s deep seismicity decreased at the beginning of 2002. The coincidence between the transition from deflation to inflation at Campi Flegrei and the onset of deflation below Vesuvio may suggest the possible transfer of magma and/or magmatic fluids between the two plumbing systems.
Analysis of Short-Term Drought Episodes Using Sentinel-3 SLSTR Data under a Semi-Arid Climate in Lower Eastern Kenya
Authors: Musyimi Peter K. , Sahbeni Ghada , Timár Gábor , Weidinger Tamás , Székely Balázs
Published: June 2023

This study uses Sentinel-3 SLSTR data to analyze short-term drought events between 2019 and 2021. It investigates the crucial role of vegetation cover, land surface temperature, and water vapor amount associated with drought over Kenya’s lower eastern counties. Therefore, three essential climate variables (ECVs) of interest were derived, namely Land Surface Temperature (LST), Fractional Vegetation Cover (FVC), and Total Column Water Vapor (TCWV). These features were analyzed for four counties between the wettest and driest episodes in 2019 and 2021. The study showed that Makueni and Taita Taveta counties had the highest density of FVC values (60–80%) in April 2019 and 2021. Machakos and Kitui counties had the lowest FVC estimates of 0% to 20% in September for both periods and between 40% and 60% during wet seasons. As FVC is a crucial land parameter for sequestering carbon and detecting soil moisture and vegetation density losses, its variation is strongly related to drought magnitude. The land surface temperature has drastically changed over time, with Kitui and Taita Taveta counties having the highest estimates above 20 °C in 2019. A significant spatial variation of TCWV was observed across different counties, with values less than 26 mm in Machakos county during the dry season of 2019, while Kitui and Taita Taveta counties had the highest estimates, greater than 36 mm during the wet season in 2021. Land surface temperature variation is negatively proportional to vegetation density and soil moisture content, as non-vegetated areas are expected to have lower moisture content. Overall, Sentinel-3 SLSTR products provide an efficient and promising data source for short-term drought monitoring, especially in cases where in situ measurement data are scarce. ECVs-produced maps will assist decision-makers with a better understanding of short-term drought events as well as soil moisture loss episodes that influence agriculture under arid and semi-arid climates. Furthermore, Sentinel-3 data can be used to interpret hydrological, ecological, and environmental changes and their implications under different environmental conditions.
Global Cloudiness and Cloud Top Information from AVHRR in the 42-Year CLARA-A3 Climate Data Record Covering the Period 1979–2020
Authors: Karlsson Karl-Göran , Devasthale Abhay , Eliasson Salomon
Published: June 2023

This paper investigates the quality of global cloud fraction and cloud-top height products provided by the third edition of the CM SAF cLoud, Albedo and surface RAdiation dataset from the AVHRR data (CLARA-A3) climate data record (CDR) produced by the EUMETSAT Climate Monitoring Satellite Application Facility (CM SAF). Compared with with CALIPSO–CALIOP cloud lidar data and six other cloud CDRs, including the predecessor CLARA-A2, CLARA-A3 has improved cloud detection, especially over ocean surfaces, and improved geographical variation and cloud detection efficiency. In addition, CLARA-A3 exhibits remarkable improvements in the accuracy of its global cloud-top height measurements. For example, in tropical regions, previous underestimations for high-level clouds are reduced by more than 2 km. By taking advantage of more realistic descriptions of global cloudiness, this study attempted to estimate trends in the observable fraction of low-level clouds, acknowledging their importance in producing a net climate cooling effect. The results were generally inconclusive in the tropics, mainly due to the interference of El Nino modes during the period under study. However, the analysis found small negative trends over oceanic surfaces outside the core tropical region. Further studies are needed to verify the significance of these results.
Rooftop Photovoltaic Energy Production Estimations in India Using Remotely Sensed Data and Methods
Authors: Kumar Anil , Kosmopoulos Panagiotis , Kashyap Yashwant , Gautam Rupam
Published: June 2023

We investigate the possibility of estimating global horizontal irradiance (GHI) in parallel to photovoltaic (PV) power production in India using a radiative transfer model (RTM) called libRadtran fed with satellite information on the cloud and aerosol conditions. For the assessment of PV energy production, we exploited one year’s (January–December 2018) ground-based real-time measurements of solar irradiation GHI via silicon irradiance sensors (Si sensor), along with cloud optical thickness (COT). The data used in this method was taken from two different sources, which are EUMETSAT’s Meteosat Second Generation (MSG) and aerosol optical depth (AOD) from Copernicus Atmospheric Monitoring Services (CAMS). The COT and AOD are used as the main input parameters to the RTM along with other ones (such as solar zenith angle, Ångström exponent, single scattering albedo, etc.) in order to simulate the GHI under all sky, clear (no clouds), and clear-clean (no clouds and no aerosols) conditions. This enabled us to quantify the cloud modification factor (CMF) and aerosol modification factor (AMF), respectively. Subsequently, the whole simulation is compared with the actual recorded data at four solar power plants, i.e., Kazaria Thanagazi, Kazaria Ceramics, Chopanki, and Bhiwadi in the Alwar district of Rajasthan state, India. The maximum monthly average attenuation due to the clouds and aerosols are 24.4% and 11.3%, respectively. The energy and economic impact of clouds and aerosols are presented in terms of energy loss (EL) and financial loss (FL). We found that the maximum EL in the year 2018 due to clouds and aerosols were 458 kWh m−2 and 230 kWh m−2, respectively, observed at Thanagazi location. The results of this study highlight the capabilities of Earth observations (EO), in terms not only of accuracy but also resolution, in precise quantification of atmospheric effect parameters. Simulations of PV energy production using EO data and techniques are therefore useful for real-time estimates of PV energy outputs and can improve energy management and production inspection. Success in such important venture, energy management, and production inspections will become much easier and more effective.
Testing Textural Information Base on LiDAR and Hyperspectral Data for Mapping Wetland Vegetation: A Case Study of Warta River Mouth National Park (Poland)
Authors: Jarocińska Anna , Niedzielko Jan , Kopeć Dominik , Wylazłowska Justyna , Omelianska Bozhena , Charyton Jakub
Published: June 2023

One of the key issues in wetland monitoring is mapping vegetation. Remote sensing and machine learning are used to acquire vegetation maps, which, due to the development of sensors and data processing and analysis methods, have increasingly high accuracy. The objectives of this study were to test: (i) which of the textural information (TI) features have the highest information potential for identifying wetland communities; and (ii) whether the use of TI improves the accuracy of wetland communities mapping using hyperspectral (HS) and Airborne Laser Scanning (ALS) data. The analysis indicated that the mean and entropy features of the Gray Level Co-occurrence Matrix had the highest potential to differentiate between various wetland communities. Adding these features to the dataset resulted in a small increase (0.005) in average F1 accuracy based on HS data and 0.011 for HS and ALS scenarios in wetland communities classification, and adding TI improved the delineation of patch boundaries. A higher increase was noted for forest and scrub vegetation (by 0.019 for the HS scenario and 0.022 for the HS and ALS scenario) and rushes (only for the HS and ALS scenario 0.017). It can be concluded that it is reasonable to use textural information for mapping wetland communities, especially for areas with a high proportion of scrub and forest and rushes vegetation included in the analysis.
Visible and Near-Infrared Hyperspectral Diurnal Variation Calibration for Corn Phenotyping Using Remote Sensing
Authors: Zhang Jinnuo , Ma Dongdong , Wei Xing , Jin Jian
Published: June 2023

Remote sensing coupled with hyperspectral technology has become increasingly popular to investigate plant traits, showcasing its advantages in studying plant growth, health, and productivity. The quality of the collected hyperspectral images is crucial for subsequent data analysis and plant phenotyping studies. However, diurnal variations in spectral characteristics introduce more data variance in canopy reflectance spectra, raising the cost of subsequent analyses and compromising the performance of trait estimation models. In this study, a fixed gantry platform in a cornfield was used to capture visible and near-infrared (VNIR) hyperspectral images of corn canopies at consecutive time intervals. By applying reference board calibration and locally weighted scatterplot smoothing to minimize the effects of ambient light and daily growth, diurnal spectral changes across all involved VNIR wavelengths were investigated. Several distinct diurnal patterns were observed to have close connections with the plants’ physiological effects. Diurnal calibration models were established at every wavelength by employing the least squares polynomial algorithm, with the highest coefficient of determination reaching 0.84. Moreover, by employing diurnal calibration in canopy spectra processing, the reduction in spectral variance brought about by varying imaging time was evidently exhibited. This study not only reveals the diurnal spectral variation pattern at VNIR bands but also offers a reliable, straightforward, and low-cost approach to improve the quality of remote sensing data and reduce the inherent variance brought about via the different imaging times ensuring that comparable spectral analysis can be performed under relatively fair conditions.
Thermal Stability and Melting Dynamics of Bimetallic Au@Pt@Au Core-Shell Nanoparticles
Authors: Borysiuk Vadym , Lyashenko Iakov A. , Popov Valentin L.
Published: June 2023

Thermal stability is an important feature of the materials used as components and parts of sensors and other devices of nanoelectronics. Here we report the results of the computational study of the thermal stability of the triple layered Au@Pt@Au core-shell nanoparticles, which are promising materials for H2O2 bi-directional sensing. A distinct feature of the considered sample is the raspberry-like shape, due to the presence of Au nanoprotuberances on its surface. The thermal stability and melting of the samples were studied within classical molecular dynamics simulations. Interatomic forces were computed within the embedded atom method. To investigate the thermal properties of Au@Pt@Au nanoparticles, structural parameters such as Lindemann indexes, radial distribution functions, linear distributions of concentration, and atomistic configurations were calculated. As the performed simulations showed, the raspberry-like structure of the nanoparticle was preserved up to approximately 600 K, while the general core-shell structure was maintained up to approximately 900 K. At higher temperatures, the destruction of the initial fcc crystal structure and core-shell composition was observed for both considered samples. As Au@Pt@Au nanoparticles demonstrated high sensing performance due to their unique structure, the obtained results may be useful for the further design and fabrication of the nanoelectronic devices that are required to work within a certain range of temperatures.