bluuebunny/crossref_metadata_2025 huggingface dataset:
Identification of Powdery Mildew Resistance-Related Genes in Butternut Squash (Cucurbita moschata)
Authors: Fu Yiqian , Hu Yanping , Yang Jingjing , Liao Daolong , Liu Pangyuan , Wen Changlong , Yun Tianhai
Published: October 2024

Powdery mildew infection is a significant challenge in butternut squash (Cucurbita moschata) production during winter in Hainan, China. The tropical climate of Hainan promotes powdery mildew infection, resulting in substantial yield losses. By utilizing transcriptome and genome sequencing data, SNPs and potential genes associated with powdery mildew resistance in butternut squash were identified. The analysis of differentially expressed genes (DEGs) following powdery mildew infection revealed several genes involved in resistance, with particular focus on a resistance (R) gene cluster that may be linked to the observed resistance. Two MLO genes in clade V from Cucurbita moschata may not be directly associated with resistance in the two genotypes studied. These findings are expected to contribute to the development of genetic tools for improving powdery mildew resistance in Cucurbita crops, thereby reducing yield losses and enhancing the sustainability of butternut squash production in Hainan and other regions.
Implementation of an Immunoassay Based on the MVA-T7pol-Expression System for Rapid Identification of Immunogenic SARS-CoV-2 Antigens: A Proof-of-Concept Study
Authors: Kumar Satendra , Nan Liangliang , Kalodimou Georgia , Jany Sylvia , Freudenstein Astrid , Brandmüller Christine , Müller Katharina , Girl Philipp , Ehmann Rosina , Guggemos Wolfgang , Seilmaier Michael , Wendtner Clemens-Martin , Volz Asisa , Sutter Gerd , Fux Robert , Tscherne Alina
Published: October 2024

The emergence of hitherto unknown viral pathogens presents a great challenge for researchers to develop effective therapeutics and vaccines within a short time to avoid an uncontrolled global spread, as seen during the coronavirus disease 2019 (COVID-19) pandemic. Therefore, rapid and simple methods to identify immunogenic antigens as potential therapeutical targets are urgently needed for a better pandemic preparedness. To address this problem, we chose the well-characterized Modified Vaccinia virus Ankara (MVA)-T7pol expression system to establish a workflow to identify immunogens when a new pathogen emerges, generate candidate vaccines, and test their immunogenicity in an animal model. By using this system, we detected severe acute respiratory syndrome (SARS) coronavirus 2 (SARS-CoV-2) nucleoprotein (N)-, and spike (S)-specific antibodies in COVID-19 patient sera, which is in line with the current literature and our observations from previous immunogenicity studies. Furthermore, we detected antibodies directed against the SARS-CoV-2-membrane (M) and -ORF3a proteins in COVID-19 patient sera and aimed to generate recombinant MVA candidate vaccines expressing either the M or ORF3a protein. When testing our candidate vaccines in a prime-boost immunization regimen in humanized HLA-A2.1-/HLA-DR1-transgenic H-2 class I-/class II-knockout mice, we were able to demonstrate M- and ORF3a-specific cellular and humoral immune responses. Hence, the established workflow using the MVA-T7pol expression system represents a rapid and efficient tool to identify potential immunogenic antigens and provides a basis for future development of candidate vaccines.
Ultra-Pressurized Deposition of Hydrophobic Chitosan Surface Coating on Wood for Fungal Resistance
Authors: Facchi Suelen P. , de Almeida Débora A. , Abrantes Karen K. B. , Rodrigues Paula C. dos S. , Tessmann Dauri J. , Bonafé Elton G. , da Silva Marcelo F. , Gashti Mazeyar P. , Martins Alessandro F. , Cardozo-Filho Lúcio
Published: October 2024

Fungi (Neolentinus lepideus, Nl, and Trametes versicolor, Tv) impart wood rot, leading to economic and environmental issues. To overcome this issue, toxic chemicals are commonly employed for wood preservation, impacting the environment and human health. Surface coatings based on antimicrobial chitosan (CS) of high molar mass (145 × 105 Da) were tested as wood preservation agents using an innovative strategy involving ultra-pressurizing CS solutions to deposit organic coatings on wood samples. Before coating deposition, the antifungal activity of CS in diluted acetic acid (AcOOH) solutions was evaluated against the rot fungi models Neolentinus lepideus (Nl) and Trametes versicolor (Tv). CS effectively inhibited fungal growth, particularly in solutions with concentrations equal to or higher than 0.125 mg/mL. Wood samples (Eucalyptus sp. and Pinus sp.) were then coated with CS under ultra-pressurization at 70 bar. The polymeric coating deposition on wood was confirmed through X-ray photoelectron spectroscopy (XPS), energy dispersive X-ray spectroscopy (EDS), scanning electron microscopy (SEM) images, and water contact angle measurements. Infrared spectroscopy (FTIR) spectra of the uncoated and coated samples suggested that CS does not penetrate the bulk of the wood samples due to its high molar mass but penetrates in the surface pores, leading to its impregnation in wood samples. Coated and uncoated wood samples were exposed to fungi (Tv and Nl) for 12 weeks. In vivo testing revealed that Tv and Nl fungi did not grow on wood samples coated with CS, whereas the fungi proliferated on uncoated samples. CS of high molar mass has film-forming properties, leading to a thin hydrophobic film on the wood surface (water contact angle of 118°). This effect is mainly attributed to the high molar mass of CS and the hydrogen bonding interactions established between CS chains and cellulose. This hydrophobic film prevents water interaction, resulting in a stable coating with insignificant leaching of CS after the stability test. The CS coating can offer a sustainable strategy to prevent wood degradation, overcoming the disadvantages of toxic chemicals often used as wood preservative agents.
ddPCR Overcomes the CRISPR-Cas13a-Based Technique for the Detection of the BRAF p.V600E Mutation in Liquid Biopsies
Authors: Palacín-Aliana Irina , García-Romero Noemí , Carrión-Navarro Josefa , Puig-Serra Pilar , Torres-Ruiz Raul , Rodríguez-Perales Sandra , Viñal David , González-Rumayor Víctor , Ayuso-Sacido Ángel
Published: October 2024

The isolation of circulating tumoral DNA (ctDNA) present in the bloodstream brings about the opportunity to detect genomic aberrations from the tumor of origin. However, the low amounts of ctDNA present in liquid biopsy samples makes the development of highly sensitive techniques necessary to detect targetable mutations for the diagnosis, prognosis, and monitoring of cancer patients. Here, we employ standard genomic DNA (gDNA) and eight liquid biopsy samples from different cancer patients to examine the newly described CRISPR-Cas13a-based technology in the detection of the BRAF p.V600E actionable point mutation and appraise its diagnostic capacity with two PCR-based techniques: quantitative Real-Time PCR (qPCR) and droplet digital PCR (ddPCR). Regardless of its lower specificity compared to the qPCR and ddPCR techniques, the CRISPR-Cas13a-guided complex was able to detect inputs as low as 10 pM. Even though the PCR-based techniques have similar target limits of detection (LoDs), only the ddPCR achieved a 0.1% variant allele frequency (VAF) detection with elevated reproducibility, thus standing out as the most powerful and suitable tool for clinical diagnosis purposes. Our results also demonstrate how the CRISPR-Cas13a can detect low amounts of the target of interest, but its base-pair specificity failed in the detection of actionable point mutations at a low VAF; therefore, the ddPCR is still the most powerful and suitable technique for these purposes.
Metabolomic Analysis of Histological Composition Variability of High-Grade Serous Ovarian Cancer Using 1H HR MAS NMR Spectroscopy
Authors: Skorupa Agnieszka , Klimek Mateusz , Ciszek Mateusz , Pakuło Sławomir , Cichoń Tomasz , Cichoń Bartosz , Boguszewicz Łukasz , Witek Andrzej , Sokół Maria
Published: October 2024

In this work, the HR MAS NMR (high-resolution magic-angle spinning nuclear magnetic resonance) spectroscopy technique was combined with standard histological examinations to investigate the metabolic features of high-grade serous ovarian cancer (HGSOC) with a special focus on the relation between a metabolic profile and a cancer cell fraction. The studied group consisted of 44 patients with HGSOC and 18 patients with benign ovarian tumors. Normal ovarian tissue was also excised from 13 control patients. The metabolic profiles of 138 tissue specimens were acquired on a Bruker Avance III 400 MHz spectrometer. The NMR spectra of the HGSOC samples could be discriminated from those acquired from the non-transformed tissue and were shown to depend on tumor purity. The most important features that differentiate the samples with a high fraction of cancer cells from the samples containing mainly fibrotic stroma are the increased intensities in the spectral regions corresponding to phosphocholine/glycerophosphocholine, phosphoethanolamine/serine, threonine, uridine nucleotides and/or uridine diphosphate (UDP) nucleotide sugars. Higher levels of glutamine, glutamate, acetate, lysine, alanine, leucine and isoleucine were detected in the desmoplastic stroma within the HGSOC lesions compared to the stroma of benign tumors. The HR MAS NMR analysis of the metabolic composition of the epithelial and stromal compartments within HGSOC contributes to a better understanding of the disease’s biology.
Integrated Biological Experiments and Proteomic Analyses of Nicotiana tabacum Xylem Sap Revealed the Host Response to Tomato Spotted Wilt Orthotospovirus Infection
Authors: Feng Hongping , Mon Waiwai , Su Xiaoxia , Li Yu , Zhang Shaozhi , Zhang Zhongkai , Zheng Kuanyu
Published: October 2024

The plant vascular system is not only a transportation system for delivering nutrients but also a highway transport network for spreading viruses. Tomato spotted wilt orthotospovirus (TSWV) is among the most destructive viruses that cause serious losses in economically important crops worldwide. However, there is minimal information about the long-distance movements of TSWV in the host plant vascular system. In this this study, we confirm that TSWV virions are present in the xylem as observed by transmission electron microscopy (TEM). Further, a quantitative proteomic analysis based on label-free methods was conducted to reveal the uniqueness of protein expression in xylem sap during TSWV infection. Thus, this study identified and quantified 3305 proteins in two groups. Furthermore, TSWV infection induced three viral structural proteins, N, Gn and Gc, and 315 host proteins differentially expressed in xylem (163 up-regulated and 152 down-regulated). GO enrichment analysis showed up-regulated proteins significantly enriched in homeostasis, wounding, defense response, and DNA integration terms, while down-regulated proteins significantly enriched in cell wall biogenesis/xyloglucan metabolic process-related terms. KEGG enrichment analysis showed that the differentially expressed proteins (DEPs) were most strongly associated with plant-pathogen interaction, MAPK signaling pathway, and plant hormone signal transduction. Cluster analysis of DEPs function showed the DEPs can be categorized into cell wall metabolism-related proteins, antioxidant proteins, PCD-related proteins, host defense proteins such as receptor-like kinases (RLKs), salicylic acid binding protein (SABP), pathogenesis related proteins (PR), DNA methylation, and proteinase inhibitor (PI). Finally, parallel reaction monitoring (PRM) validated 20 DEPs, demonstrating that the protein abundances were consistent between label-free and PRM data. Finally, 11 genes were selected for RT-qPCR validation of the DEPs and label-free-based proteomic analysis concordant results. Our results contribute to existing knowledge on the complexity of host plant xylem system response to virus infection and provide a basis for further study of the mechanism underlying TSWV long-distance movement in host plant vascular system.
GDF-15 and mtDNA Deletions Are Useful Biomarkers of Mitochondrial Dysfunction in Insulin Resistance and PCOS
Authors: Varhegyi Vera , Modos Anna , Trager Domonkos , Gerszi Dora , Horvath Eszter Maria , Sipos Miklos , Acs Nandor , Molnar Maria Judit , Varbiro Szabolcs , Gal Aniko
Published: October 2024

There is no literature available about the growth differentiation factor-15 (GDF-15) biomarker in combination with mitochondrial DNA (mtDNA) deletions in insulin resistance (IR), and polycystic ovary syndrome (PCOS); however, it would be useful to achieve optimal metabolic status and improve pregnancy success. In this study, the role of GDF-15 and mtDNA deletions as biomarkers in the pathogenesis of IR and PCOS was investigated. In our study, 81 female patients who were treated for IR and/or PCOS and 41 healthy controls were included. GDF-15 levels in patients showed a marked increase compared to controls. Elevated GDF-15 levels were found in 12 patients; all of them had a BMI > 25 kg/m2, which is associated with reactive hyperinsulinemia. The presence of mitochondrial dysfunction was mainly observed in the IR-only subgroup. The increase in plasma levels of GDF-15 and the prevalence of mtDNA deletions is directly proportional to body mass index. The more marked metabolic abnormalities required more intensive drug therapy with a parallel increase in plasma GDF-15 levels. Elevated levels of GDF-15 and the presence of mitochondrial DNA deletions may be a consequence of carbohydrate metabolism disorders in patients and thus a predictor of the process of accelerated aging.
In Vitro Inhibition of Rhizoctonia oryzae-sativae Using Bacterial Strains as a Sustainable Alternative for Controlling Sheath Blight in Rice
Authors: Quiñones-Pezo Liz Cheril , Ríos-Ruiz Winston Franz , Pompa-Vásquez Danny Fran , Rios-Reategui Franz , Hernández-Amasifuen Angel David , Corazón-Guivin Mike Anderson
Published: October 2024

The control of ‘sheath blight’ in rice (Oryza sativa L.), which is caused by the fungus Rhizoctonia oryzae-sativae, has become problematic due to the excessive application of fungicides and their consequent harmful effects. Hence, the search for less contaminating alternatives to conventional chemical products is necessary. This study evaluated the in vitro inhibitory activity of four bacterial strains on the growth of R. oryzae-sativae in both tryptone soy broth (TSB) and mineral medium (MM). The results demonstrated that all evaluated strains (Bacillus tequilensis SMNCT17-02, Priestia aryabhattai SMNCH17-07, Burkholderia vietnamiensis TUR04-01, and Burkholderia vietnamiensis TUR04-03) inhibited the growth of R. oryzae-sativae. Specifically, the activity of B. tequilensis SMNCT17-02 resulted in the smallest area of R. oryzae-sativae growth in both TSB medium (8.54 cm2) and MM (5.53 cm2), suggesting a notable antifungal effect. When evaluating the action of supernatants generated by the growth of the four bacterial strains in TSB and MM culture media, significant inhibition of R. oryzae-sativae growth was only observed for supernatants produced in MM. This inhibition was attributed to the presence of soluble secondary metabolites. These results offer new perspectives in biotechnology, suggesting the possibility of developing effective products based on easily extractable soluble secondary metabolites, thus promoting sustainable agriculture.
Identification of Essential Features in Developing a Novel Femoral Stem Reflecting Anatomical Features of East Asian Population: A Morphological Study
Authors: Bahk Ji Hoon , Han Seung-Beom , Rhyu Kee Hyung , Yoo Jeong Joon , Lim Seung-Jae , Park Kwan Kyu , Kim Sang-Min , Lim Young Wook
Published: October 2024

Background: Recent advancements in hip arthroplasty aim to enhance the stability, longevity, and functionality of femoral implants. However, the distal fitting of femoral stems, often caused by metaphyseal–diaphyseal mismatch, remains a significant issue, particularly in patients with Dorr type A femora. Such mismatches can result in suboptimal implant performance, leading to potential complications. This study focuses on evaluating the anatomical compatibility of five representative single-tapered wedge mid–short stems with the mediolateral (ML) anatomy of the proximal femur in an East Asian population, where these mismatches are often more pronounced. Methods: A total of 742 patients from two hospitals, all of whom underwent unilateral primary total hip arthroplasty, were included in the study. The contralateral proximal femur was confirmed to have normal anatomy in each patient. Hip anteroposterior radiographs were used for measurements, which were standardized in conjunction with CT images. Key anatomical parameters were measured, including proximal and distal medial–lateral canal dimensions, vertical offset, and medial offset. Five femoral stem designs—Tri-lock®, Taperloc®, Anthology®, Accolade II®, and Fit®—were evaluated. R programming was employed for a detailed fit analysis to match stem sizes with patient anatomy, categorizing the fit as proximal, simultaneous proximal–distal, or distal engagement. Results: Among the femoral stems analyzed, the Fit® stem demonstrated the closest alignment with the regression line for ML widths in the study population (slope = 0.69; population ML slope = 0.38). This was followed by Accolade II®, which had a slope of 0.83. In terms of offset options, the Accolade II® offered the largest offset coverage, making it particularly suitable for this population. The fit analysis revealed that the Fit® stem had the highest suitable fit rate (90.56%), followed by Accolade II® (73.04%). Taperloc®, Anthology®, and Tri-lock® had similar fit rates of approximately 59%. Overall, optimal results were obtained for 92.05% of the population in the automated fitting trial, regardless of the product type. Conclusions: When designing modern cementless femoral stems intended for press-fit fixation, it is crucial to account for the anatomical variations specific to the target population. In this study, Fit® and Accolade II® femoral components demonstrated superior compatibility with the femoral anatomy of the East Asian population, particularly in those with a higher incidence of Dorr type A femora. These stems, characterized by slimmer distal dimensions and high-offset options, appear to minimize metaphyseal–diaphyseal mismatch and associated complications.
Management of Acute Coronary Syndrome in Elderly Patients: A Narrative Review through Decisional Crossroads
Authors: Verardi Roberto , Iannopollo Gianmarco , Casolari Giulia , Nobile Giampiero , Capecchi Alessandro , Bruno Matteo , Lanzilotti Valerio , Casella Gianni
Published: October 2024

Diagnosis and treatment of acute coronary syndrome (ACS) pose particular challenges in elderly patients. When high troponin levels are detected, the distinction between non-ischemic myocardial injury (NIMI), type 1, and type 2 myocardial infarction (MI) is the necessary first step to guide further care. However, the assessment of signs of ischemia is hindered in older patients, and no simple clinical or laboratory tool proved useful in this discrimination task. Current evidence suggests a benefit of an invasive vs. conservative approach in terms of recurrence of MI, with no significant impact on mortality. In patients with multivessel disease in which the culprit lesion has been treated, a physiology-guided complete percutaneous revascularization significantly reduced major events. The management of ACS in elderly patients is an example of the actual need for a multimodal, thorough clinical approach, coupled with shared decision-making, in order to ensure the best treatment and avoid futility. Such a need will likely grow throughout the next decades, with the aging of the world population. In this narrative review, we address pivotal yet common questions arising in clinical practice while caring for elderly patients with ACS.
Clinical Trends and Outcomes in Technology-Assisted Total Hip Arthroplasty
Authors: Oikonomou Konstantinos , Kiritsis Nicholas R. , Hopper Haleigh M. , Satalich James R. , O’Neill Conor N. , Ernst Brady , Satpathy Jibanananda
Published: October 2024

Background/Objectives: In recent years, there has been a widespread focus on implementing technology in total hip arthroplasty (THA) to further improve precision and outcomes. This study aimed to identify recent trends in the utilization, clinical variables, and rate of adverse events for technology-assisted THA (TA-THA) and compare the outcomes to those of conventional THA. Methods: This retrospective cohort analysis of the ACS-NSQIP database queried data on THA patients (CPT 27130) from 2015 to 2020. Technology assistance was identified with CPT 20985, 0054T, and 0055T. Matched cohorts were created to compare clinical comorbidities and adverse events. Results: This analysis included 219,216 conventional THAs and 2258 cases utilizing TA-THA. The number and percentage of surgeries utilizing technology, as well as the average operative time, consistently rose from 2015 to 2019, with all declining in 2020. Length of stay decreased yearly from 2015 to 2019, with an increase in 2020. There were no significant differences in the incidence of adverse events by year. Matched cohort analysis demonstrated that TA-THA led to longer operative times (102.6 ± 35.6 vs. 91.6 ± 37.4 min, p < 0.001) and a shorter average length of stay (1.6 ± 1.4 vs. 2.0 ± 1.9 days, p < 0.001). Transfusion rates were higher in the TA-THA cohort (6.0% vs. 4.4%, p = 0.013). Conclusions: The usage of TA-THA increased from 2015 to 2019, with declines during 2020. TA-THA led to longer operative times, increased transfusion rates, and no difference in the incidence of adverse events compared to conventional arthroplasty. These findings demonstrate that TA-THA is growing in popularity without a significant improvement in short-term complication rates.
Insights into Retinal Metastasis from Systemic Carcinoma: A Systematic Review of Clinical and Multimodal Imaging Characteristics
Authors: Wang Ling-Uei , Hsieh Tzu-Han , Chen Fang-Ting , Chen Yun-Ju , Wang Jia-Kang , Hsu Yung-Ray
Published: October 2024

Background/Objectives: Retinal metastasis (RM) is an exceptionally rare clinical condition, comprising less than 1% of ocular metastasis cases. This study aims to investigate the clinical features of RM originating from systemic carcinoma. Methods: A systematic review (PROSPERO ID: CRD42022344724). Results: The systematic review revealed 58 cases involving 61 eyes. Most of the cases (82.8%) had a known primary malignancy before RM was diagnosed. The main primary sites were the lung (45.8%), gastrointestinal tract (23.7%), and breast (16.9%). The lung was the most common (80.0%) carcinoma in cases with unknown primary sites. The median survival after RM diagnosis was 11 months. The main clinical patterns were patchy retinal infiltrates (35.7%), an elevated retinal mass (63.8%), and punctate retinal infiltrates (7.1%). Subretinal fluid, vitreous seeding, and choroidal invasion were noted in 57.4%, 41.0%, and 6.6% of eyes, respectively. Key multimodal imaging features were early hypofluorescence with mid-to-late hyperfluorescence on fluorescein angiography (84.6%) and hyper-reflectiveness on optical coherence tomography (70.8%). A final best-corrected visual acuity of ≤20/200 was noted in 66.7% of the eyes. Conclusions: Patchy retinal infiltrates, an elevated retinal mass, and punctate retinal infiltrates are the major clinical patterns of RM from systemic carcinoma. The primary carcinoma sites are the lung, gastrointestinal tract, and breast. Systemic and visual prognoses were unfavorable.
The Diagnosis and Management of Chronic Constipation in Italy: Results from a Survey Conducted among Italian Gastroenterologists
Authors: Lambiase Christian , D’Alba Lucia , Galeazzi Francesca , Bassotti Gabrio , Consalvo Danilo , Battaglia Edda , Cataudella Giovanni , Neri Maria Cristina , Londoni Claudio , Rossitti Piera , Valenzi Emiliano , Annibale Bruno , Soncini Marco , Parodi Maria Caterina , Bellini Massimo
Published: October 2024

Background: Chronic constipation (CC) is one of the most common disorders of gut–brain interaction (DGBI). The management of CC requires specific skills due to its complex and multifactorial pathophysiology and its multistep treatment. The aims of this study were to evaluate the availability and the use of diagnostic tools for CC in Italy and the therapeutic management of CC by Italian gastroenterologists (GEs). Methods: A survey was conducted during the 28th meeting of the Italian Federation of Digestive Disease Societies (FISMAD; Rome, Italy, 11–14 May 2022). The survey explored the presence of a clinic dedicated to DGBIs, the availability and the use of specific diagnostic tools, the routine use of digital rectal examination (DRE), and the therapeutic approach to CC by Italian GEs. Results: The survey was taken by 236 GEs. The most significant results were that 42% of respondents had a clinic dedicated to DGBI in their institute; DRE was regularly performed by 56.8% of GEs when evaluating a CC patient; young GEs (≤40 years) performed DRE less frequently than older ones (p < 0.001); anorectal manometry was available to 44.3% of GEs; balloon expulsion test (BET) was available to 19.1% of GEs; GEs with a clinic dedicated to DGBI had more frequent access to anorectal physiology testing (p < 0.001); diet and lifestyle advice were the most frequently prescribed treatments; and fiber and macrogol were the second and third most prescribed treatments, respectively. Conclusions: The survey provides an interesting picture of CC management by Italian GEs. The results are in line with previous data collected about 10 years ago among Italian GEs (“CHRO.CO.DI.T.E study”); DRE is still rarely performed by Italian GEs (particularly by young GEs). The availability of anorectal physiology testing is still limited, and BET, which could be easily performed in everyday clinical settings, is rarely performed. Lifestyle suggestions, macrogol and fiber are the preferred treatment, as recommended by all guidelines. These results will be useful to identify as yet unmet educational needs and critical issues to improve CC management.
Enhanced Self-Checkout System for Retail Based on Improved YOLOv10
Authors: Tan Lianghao , Liu Shubing , Gao Jing , Liu Xiaoyi , Chu Linyue , Jiang Huangqi
Published: October 2024

With the rapid advancement of deep learning technologies, computer vision has shown immense potential in retail automation. This paper presents a novel self-checkout system for retail based on an improved YOLOv10 network, aimed at enhancing checkout efficiency and reducing labor costs. We propose targeted optimizations for the YOLOv10 model, incorporating the detection head structure from YOLOv8, which significantly improves product recognition accuracy. Additionally, we develop a post-processing algorithm tailored for self-checkout scenarios, to further enhance the application of the system. Experimental results demonstrate that our system outperforms existing methods in both product recognition accuracy and checkout speed. This research not only provides a new technical solution for retail automation but offers valuable insights into optimizing deep learning models for real-world applications.
Insights into the Vibration Characteristics of Spatial Radial Gate Affected by Fluid–Structure Interaction
Authors: Liu Feng , Xu Chao , Liu Min , Yi Ruiji , Zhang Yu
Published: October 2024

Radial gate, a spatial frame structure, is the key factor to control water discharge in dam structure and storm surge barriers. However, the fluid-induced vibration (FIV) problem always occurs owing to fluctuation loads exerted on the gate, threatening the safety of hydropower stations. In this work, two fluid–structure interaction (FSI) modal analysis methods—the coupled acoustics–structure method and the added-mass method—are provided. Further, a comprehensive investigation on the vibration characteristics of the spatial radial gate, considering spatial structural characteristics and the FSI effect, is conducted. The numerical results revealed that the feasibility of the proposed coupled acoustics–structure method in analyzing FSI modal analysis was demonstrated; moreover, a reasonable length of the fluid domain in front of the skinplate existed for efficient computation. Meanwhile, through the added-mass method, the rational added-mass discount factor of hydrodynamic loads obtained from the Westergaard formula was provided. The FSI effect induced whole-gate rotation vibration streamwise around trunnion pins, significantly reducing the gate’s fundamental vibration frequency. In addition, three typical dynamic-instability vibration patterns of radial gates were presented. These patterns were affected by spatial structural characteristics and FSI. It was demonstrated that the struts and skinplate coupled bending–torsional vibration would cause the radial gate frame structure to fail catastrophically. The proposed insights can provide guidelines of vibration characteristics analysis of the radial gate submerged in flow water in reservoir and storm surge barriers.
The Development of a Fillet Design Tool Based on Light-Weight Marine Diesel Engine Crankshafts
Authors: Chen Guangku , Wang Guixin , Liu Shuaining , Zhu Jialiang , Niu Xiaoxiao , Wang Yinyan
Published: October 2024

As modern diesel engine design progresses toward higher burst pressure and power density, strict performance indices impose greater demands on the structural strength and reliability of crankshafts. We integrated finite element analysis and strength testing methods to achieve a lightweight crankshaft design. A comparison of the simulated results with the test data revealed that the crankshaft safety factor surpassed the permissible safety factor by 3.5 times, demonstrating significant safety redundancy in the design. We employed topology optimization techniques to create various crankshaft optimization models, yielding near-optimal solutions. Consequently, we identified the crankshaft with the best overall performance following comparative evaluations. We examined the influence of the fillet structure on the safety factor to mitigate stress concentration issues. Through multibody dynamic fatigue analysis, optimizing the crankshaft fillet resulted in a 6~7% increase in the safety factor. The minimum safety factor for the designed crankshaft was 1.6 times higher than the material permissible safety factor, which was 1.15. Utilizing the developed transient dynamics model of the lightweight crankshaft and a backpropagation genetic algorithm, we created a crankshaft fillet design tool to streamline the design process, which holds significant importance for the marine engine sector.
Sensitivity Analysis of Mooring Chain Fatigue of Floating Offshore Wind Turbines in Shallow Water
Authors: Chen Jiahao , Yin Ziwei , Zheng Can , Li Yan
Published: October 2024

Shallow-water mooring chains of floating offshore wind turbines pose challenges such as nonlinear tension characteristics, high cost and prominent fatigue issues, which seriously hinder the large-scale use of floating wind power. Based on the hydrological and meteorological data of an offshore wind farm in the South China Sea, a sensitivity analysis of the mooring chain fatigue of a semi-submersible floating offshore wind turbine in shallow water was conducted with different environmental loads and mooring chain design parameters. It was found that mooring fatigue is mainly caused by the wave condition within diagonal distribution of the wave dispersion diagram. The mooring chain fatigue calculated using the main monochromatic wave was more conservative than that calculated using the wave scatter diagram with a difference of about 28%. The mooring fatigue calculated using turbulent wind spectrum was greater than that calculated using steady wind. In contrast, waves are the main source of mooring fatigue, and the second-order wave force has an undeniable impact on the mooring fatigue of a semi-submersible floating offshore wind turbine, which accounts for approximately 26% of wave-induced mooring fatigue. In addition, the current-induced mooring fatigue should not be neglected, although its effect is not as large as that caused by the wave or wind loads. An increase in the cross-sectional diameter and the length of a mooring chain, as well as a reasonable clump weight arranged, all contribute to improving the fatigue life of mooring chains in shallow water, which provide useful technical references for the optimization of mooring lines fatigue in shallow-water areas.
Three-Month Mortality in Nonhaematological Patients with Chronic Pulmonary Aspergillosis: Differences between Subtypes
Authors: González García Pablo , Fernández-Navarro Julia , Bru-Arca Mónica , Álvarez-Artero Elisa , Solís Pablo , Roiz Mesones María Pía , Muñoz Bellido Juan Luis , García Castro María Antonia , Belhassen-García Moncef , Pardo Lledías Javier
Published: October 2024

Objectives: Chronic pulmonary aspergillosis (CPA) is a fungal lung infection characterised by the slowly progressing destruction of the lung parenchyma and has four main subtypes. The objective of this work was to evaluate the epidemiology of CPA in our area and evaluate the involvement of the different subtypes in mortality. Methods: This was a descriptive longitudinal retrospective study developed in three tertiary hospitals in Spain. Among all patients admitted with a pulmonary aspergillosis diagnosis, we selected those who fulfilled the criteria for chronic aspergillosis according to the criteria of Denning, excluding those with a haematological disorder. Results: Among 409 inpatients recorded as having a pulmonary aspergillosis infection, only 76 (18.5%) fulfilled the criteria for CPA, with an estimated incidence of 0.67 cases/100,000 inhabitants/year. The subtypes detected were subacute invasive aspergillosis (SAIA) in 33 (43.4%) patients, simple aspergilloma (SA) in 25 (32.9%) patients, cavitary chronic aspergillosis (CCPA) in 13 (17.1%) patients, and chronic fibrosis (CFPA) in five (6.5%) patients. The overall three-month mortality rate was 23%, which was higher in SAIA patients. The predictors of early mortality were age > 65 years (OR 3.0 CI 95 1.0–9.5 p = 0.043) and the SAIA subtype vs. other subtypes (OR 3.1 CI 95 1.0–9.5 p = 0.042). Conclusions: The incidence rate estimated was inferior to that previously reported. The three-month mortality in patients with CPA was high, with older age and the SAIA subtype being the variable independent predictors of a worse prognosis.
Personalized Treatment Strategies via Integration of Gene Expression Biomarkers in Molecular Profiling of Laryngeal Cancer
Authors: Maniaci Antonino , Giurdanella Giovanni , Chiesa Estomba Carlos , Mauramati Simone , Bertolin Andy , Lionello Marco , Mayo-Yanez Miguel , Rizzo Paolo Boscolo , Lechien Jerome R. , Lentini Mario
Published: October 2024

Laryngeal cancer poses a substantial challenge in head and neck oncology, and there is a growing focus on customized medicine techniques. The present state of gene expression indicators in laryngeal cancer and their potential to inform tailored therapy choices are thoroughly examined in this review. We examine significant molecular changes, such as TP53, CDKN2A, PIK3CA, and NOTCH1 mutations, which have been identified as important participants in the development of laryngeal cancer. The study investigates the predictive and prognostic significance of these genetic markers in addition to the function of epigenetic changes such as the methylation of the MGMT promoter. We also go over the importance of cancer stem cell-related gene expression patterns, specifically CD44 and ALDH1A1 expression, in therapy resistance and disease progression. The review focuses on indicators, including PD-L1, CTLA-4, and tumor mutational burden (TMB) in predicting immunotherapy responses, highlighting recent developments in our understanding of the intricate interactions between tumor genetics and the immune milieu. We also investigate the potential for improving prognosis accuracy and treatment selection by the integration of multi-gene expression panels with clinicopathological variables. The necessity for uniform testing and interpretation techniques is one of the difficulties, in implementing these molecular insights into clinical practice, that are discussed. This review seeks to provide a comprehensive framework for promoting personalized cancer therapy by combining the most recent data on gene expression profiling in laryngeal cancer. Molecularly guided treatment options may enhance patient outcomes.
The Price Formation of GCC Country iShares: The Role of Unsynchronized Trading Days between the US and the GCC Markets
Authors: Al-Nassar Nassar S.
Published: October 2024

Some US-listed country exchange-traded funds (ETFs) suffer from chronic and meaningful mispricing in the form of premiums or discounts relative to their fundamental value despite the presence of the creation/redemption mechanism. This mispricing is mainly attributed to the staggered information flow due to nonoverlapping time zones between the market where the ETF is listed and its underlying home market. This study provides out-of-sample evidence on the price formation of Gulf Cooperation Council (GCC) country ETFs and gauges the impact of mispricing on their underlying home markets. The GCC context is particularly insightful because these markets have nonoverlapping time zones with the US and follow distinct trading schedules. Our sample comprises daily data from three countries’ iShares that exclusively track the Qatari, Saudi, and Emirati stock markets from 17 September 2015 to 14 March 2023. The results show that GCC ETFs are driven mainly by their net asset values (NAVs), albeit imperfectly, while the S&P500 exerts a relatively mild influence on these ETFs compared to other country ETFs, as reported by prior studies. Moreover, we find that crude oil prices positively and significantly impact GCC ETFs’ pricing. When we control for unsynchronized trading days between the US and the GCC home markets, we find a structural difference between overlapping and nonoverlapping trading days. This structural difference manifests in a sluggish adjustment to correct mispricing in the ETF market on the day the home market is closed; however, other variables, including the S&P500, show no discernible difference, which refutes the overreaction explanation. This recurrent pattern is reflected in a clear day-of-the-week pattern in the price discovery these ETFs offer to their underlying home markets.
Does Information Source Matter? Corporate Reputation Management during Negative Social Responsibility Events
Authors: Peng Hongxia , Zhang Qiang , Zhang Zhiqiang
Published: October 2024

In the era of digital marketing, where consumers and enterprises frequently interact with each other, consumers hold different attitudes toward the different sources of information, including corporate social responsibility information. Negative corporate social responsibility can have direct impacts on corporate reputation. Choosing appropriate channels to publish the negative social responsibility information of enterprises in order to reduce the impact of these negative social events on corporate reputation is imperative for corporate image management. This research examines the differences in the impact of enterprise-generated content and -co-generated content on consumer attitudes using second-hand data analysis and then investigates how different information sources influence corporate reputation through empirical experiments. The results indicate that co-generated content performs better than other sources of information on corporate reputation, while professional user-generated content has the most negative impact. We further identify the external attribution as a mediation mechanism in the relationship between information sources and corporate reputation. The theoretical contributions and managerial implications of the research findings are discussed.
Update on Current Hormonal and Non-Hormonal Contraceptive Options in Non-Human Primates
Authors: Nederlof Remco A. , Bruins-van Sonsbeek Linda G. R. , Stumpel Job B. G. , Bakker Jaco
Published: October 2024

Reproductive success in captive non-human primates (NHPs) has increased the demand for safe, effective, and reversible population control methods. This review provides an overview of the current literature on hormonal and non-hormonal contraceptives as reproductive control methods in NHPs. Where available, behavioral and welfare implications, as well as drug efficacy, reversibility, and associated adverse effects, are considered. However, a paucity of data exists for NHPs, particularly in regard to non-hormonal contraceptives, emphasizing the need for institutions to share their experiences with reproductive management techniques in the species under their care.
Spatiotemporal Evolution of Land Use Structure and Function in Rapid Urbanization: The Case of the Beijing–Tianjin–Hebei Region
Authors: Li Xiaoyang , Lu Zhaohua
Published: October 2024

The rapid increase in urbanization is accompanied by the evolution of land use structure and function. Since its reform and opening up, China has entered a stage of rapid urbanization, which has brought about higher requirements in terms of rational allocation within land use structure and the optimization of land use function. However, most existing studies have evaluated the structure and function of land use separately, resulting in a decoupling of the two, and have not accurately depicted the spatiotemporal characteristics of the evolution of land use. Here, based on statistical data and remote sensing image data, we constructed a dual evaluation index system for land use structure and function which uses the characteristics of land use structure to evaluate the property of land use function directly. We used the entropy weight method to characterize the spatiotemporal evolution of urbanization and land use structure and applied a land use function deviation degree model to discuss the evolution path for land use function. Our results showed that the dominant dimension of urbanization changed from eco-environmental urbanization to economic urbanization in the rapid economic development stage. In terms of quantity within land use structure, urban-agricultural-ecological spaces have developed in a synergistic direction. Regarding the quality of land use structure, its development level exhibited an upward trend in Beijing and Hebei, while Tianjin demonstrated a U-shaped development trajectory. With urbanization development, the dominant function of regional land use has evolved to a higher level of synergy in the Beijing–Tianjin–Hebei region. These results offer inspiration for formulating regional dynamic land use policy and phased planning of urbanization development in rapidly urbanizing regions.
Detection of the Evolution Process of Desertification in Gulang County Based on Long Series and Similar Time Images
Authors: Liu Panpan , Guo Bing , Zhang Rui
Published: October 2024

Previous studies are mostly conducted based on sparse time series and often ignore the dramatic changes in desertification during the year. Utilizing the Landsat and MODIS data sets from 2000 to 2020, this study applied the spatio-temporal fusion algorithm to obtain the images of the study area taken at similar times in August over the past 20 years. The optimal desertification remote sensing monitoring index of Gulang County was constructed based on the feature space model, and then the spatial and temporal evolution patterns and the driving mechanism of desertification in Gulang County were revealed by using a geographic detector. The research results were as follows: (1) The ESTARFM algorithm had better applicability in constructing long time series and similar time images with the correlation coefficient R2 = 0.83 between the results of the ESTARFM fusion model and the original image; (2) the SWCI-MSAVI feature space desertification monitoring index model based on point-to-point mode had the best applicability with an overall accuracy of 95.39% and a Kappa coefficient of 0.94; (3) from 2000 to 2020, the desertification showed an increasing trend, and the degree of desertification gradually intensified from south to north in Gulang County; (4) the dominant factors in various historical periods were different, which were mainly composed of precipitation, temperature and population density. The dominant interactive factors changed from alternating dominance of natural factors and human activity factors to the co-dominance of natural factors and human activity factors. The research results could provide decision-making support for precise prevention and control of desertification in arid–semi-arid regions.
Ecosystem Services Supporting Environmental Impact Assessments (EIAs): Assessments of Navigation Waterways Deepening Based on Data, Experts, and a 3D Ecosystem Model
Authors: Schernewski Gerald , Jekat Mara , Kösters Frank , Neumann Thomas , Steffen Swantje , von Thenen Miriam
Published: October 2024

The navigation waterways to the harbors of Rostock (Warnow Estuary), Germany, and Szczecin (Oder/Szczecin Lagoon), Poland, were recently deepened. Both activities required Environmental Impact Assessments. We conducted expert- and data-based ecosystem service assessments for both case studies. Additionally, we performed 3D-ecosystem model simulations. For the Oder Lagoon, the model results show that the waterway deepening increased the burial in sediments by 807 t N/a, 112 t P/a and 4661 T C/a. However, altogether, the impacts of the deepening to 12.5 m draught on the lagoon ecosystem are minor and a model application is not necessary, but the results improve the data basis for ecosystem service assessments. Our expert-based ecosystem service approach is adaptable to the needs of coastal engineering and hydraulic projects and is both easy and quick to apply and transferable. The assessment results highlight the relevance of cultural services and can complement EIAs. Our approach can especially support the early scoping stage of an EIA. It has the potential to enhance cooperation and communication with and between stakeholders, reduce conflicts, and save time. Additionally, it could improve the compilation and addressing of stakeholder concerns, potentially reducing costs associated with unnecessary studies.
The Strategic Development of Quality Improvement Land Data Incrementally Using Integrated PESTEL and SWOT Analysis in Indonesia
Authors: Huda Nurul , Hernandi Andri , Gumilar Irwan , Meilano Irwan , Cahyaningtyas Lisa A.
Published: October 2024

Land registration is an important program in asset legalization with the vast land resources owned by Indonesia. The reality is that there are 48 million certificated lands out of an estimated 126 million certificates throughout Indonesia, so the Ministry of Agrarian Affairs and Spatial Planning/National Land Agency (ATR/BPN) made a breakthrough through the Complete Systematic Land Registration (PTSL) program. The grouping mechanism of the PTSL program is divided into four clusters, namely K-1, K-2, K-3, and K-4. Land parcels included in K-4 have problems, namely that they have been registered but not mapped/mapped incorrectly, so the idea of modeling the K-4 typology is needed for accelerating the improvement in land data quality (KW). The research location is in Bandung Regency, which is included in the top five land offices with the highest number of K-4 in West Java Province. This research method uses a mixed method, namely quantitative with a Slovin approach for the identification and clustering of K-4 typology and descriptive qualitative for justification of typology modeling in multiple aspects. The results of K-4 typology modeling were 128 clusters based on seven types of data criteria and obtained 4 clusters that matched the data sample, namely T-1, T-43, T-63, and T-128. The four clusters were justified against the K-4 concept in the Ministry of ATR/BPN, the FFP-LA concept, and the PESTLE framework. Dissemination of K-4 typology modeling is a breakthrough that can be implemented by the Ministry of ATR/BPN and its staff in various regions and the role of multi-concepts in this research can be an input for improving the K-4 concept that has been in effect so far.