bluuebunny/crossref_metadata_2025 huggingface dataset:
Sustainable Development Strategy of Chinese Animation Industry
Authors: Fan Kuo-Kuang , Feng Ting-Ting
Published: June 2021

The animation industry is a crucial part of cultural and creative industries and has formed a huge consumer market all over the world and even become a pillar industry in developed countries. However, in China, the animation industry is still in its infancy, and its development is relatively backward. The main reason is that there is no sustainable development model in the industry. Thus, how to carry out sustainable development has become an important research topic for the Chinese animation industry. This study probed into the challenges and problems faced by China’s animation industry, and based on Porter’s Diamond Model, analyzed the current situation of China’s animation industry from six aspects: production factors, demand conditions, industrial chain, enterprise strategy, cultural factors, and government policies, clarifies the key direction for industrial development, and puts forward relatively comprehensive reference strategies to promote the formation of a sustainable development model for the animation industry. The research shows that the sustainable development of the animation industry must be completed by integrating resources within the industry, improving the chain, upgrading the enterprises, technological innovations, cultural embedding, external personnel training, government support, legal protection, and other aspects.
A Speed-Variant Balancing Method for Flexible Rotary Machines Based on Acoustic Responses
Authors: Peplow Andrew , Isavand Javad , Kasaei Afshar , Afzali Babak , Bard-Hagberg Delphine
Published: June 2021

As rotary machines have become more complicated, balancing processes have been classified as a vital step in condition monitoring to ensure that machines operate reliably, smoothly and safely. All rotating objects will deflect during rotation and all objects possess certain natural frequencies in the absence of rotation. However, an unbalanced object can cause significant unwanted deflection created by resonant vibration at a frequency (cycles/second) close to certain rotational speeds (rotations/second), known as critical speeds. This is especially important for flexible machines which normally work at rotations above their critical speeds. Imbalance is a common problem in flexible rotating machinery that can lead to extreme vibration and noise levels. This is one of the major reasons for studying various balancing methods applied to the vibration response of rotating machines. Recently, the relation between acoustic and vibration response during a rotary machine balancing process based on the original Four-Run method has been presented for constant speed machines. This method cannot be applied to machines in start-up or shut-off. Hence, by considering the acoustic and vibration responses of a machine between its critical speeds, this research presents a new innovative speed-variant balancing method based on the original Four-Run method, named as (PPCS) Peak to Peak for Critical Speeds. The proposed method consists of two major types of application: the first is in the run-up of the machine and the second is in shut down. Experimental laboratory results show that this method can be implemented for speed-variant and flexible rotary machines during run-up or shut-down transient processes based on acoustic and vibration measurements. Further, the results show the same trend in acoustic and vibration responses during balancing process which was shown for constant speed rotary machines. With a 40% improvement in response compared to around 55% obtained by traditional vibration measurements, the results found show an appreciable benefit in an alternative acoustic methodology that may have not been considered previously for run-up and shut-down issues. In addition, since only the magnitude of response is required and this is a non-contact technique an acoustic-only methodology, it can be employed with some confidence as an innovative and readily available method for condition monitoring.
[18F]-FDG PET/CT of the Pulmonary Benign Metastasizing Leiomyoma in a Breast Cancer Patient: A Case Report
Authors: Kao Chun-Hao , Gao Hong-Wei , Ko Kai-Hsiung , Liao Guo-Shiou , Tsai Chi-Jung
Published: June 2021

18F-fluorodeoxyglucose ([18F]-FDG) positron emission tomography/computed tomography (PET/CT) is a useful functional image technique to evaluate the disease extent in many cancers, as well as differentiate benign disease from malignant metastasis. We report the case of a 49-year-old woman with breast cancer and suspected lung metastasis by conventional images. After FDG-PET/CT demonstrating faint, or even no FDG uptake in bilateral pulmonary nodules, accompanied with obvious uterine fibroids, benign metastasizing leiomyoma (BML) of the lung was included in a differential diagnosis and finally confirmed by surgical pathology. FDG PET/CT showed its value in the diagnosis of BML in the breast cancer patient who was misleading as lung metastasis during outpatient follow-up.
A Decision Analysis Model for the Brand Experience of Branded Apps Using Consistency Fuzzy Linguistic Preference Relations
Authors: Hsu Tsuen-Ho , Chen Chun-Hsien , Yang Ya-Wun
Published: June 2021

Branded apps are not only an important platform for enterprises and customers to have real-time interactions and communicate marketing messages, but also a new business model that encourages value co-creation between the two. In order to explore the impact of branded apps on customers, this study constructs a fuzzy multi-criteria decision making (FMCDM) analysis model, and it uses consistent fuzzy linguistic preference relations (CFLPR) to set up a symmetric pairwise comparison matrix, which greatly reduces the complexity and error rate of calculations. Empirical research findings show that brand experience attributes and the influence of brand experience on customer loyalty and satisfaction can be more accurately measured. As a consequence of this study, we show that, among the brand experience facets of two retail chain branded apps, behavioral experience is the most favored, while affective experience is the least favored. Furthermore, brand attachment and active participation should be strengthened to enhance customer loyalty. Through the analytical model employed in this study, enterprises can regularly monitor changes in the brand experience preferences of branded app users and evaluate app performance to flexibly adjust mobile device-based marketing campaigns and strategies. It can also aid enterprises in using mobile devices effectively to improve customer loyalty and address the issue of diminishing brand loyalty.
New Method for Calculation of Radiation Defect Dipole Tensor and Its Application to Di-Interstitials in Copper
Authors: Demidov Dmitry N. , Sivak Alexander B. , Sivak Polina A.
Published: June 2021

The effect of external and internal elastic strain fields on the anisotropic diffusion of radiation defects (RDs) can be taken into account if one knows the dipole tensor of saddle-point configurations of the diffusing RDs. It is usually calculated by molecular statics, since the insufficient accuracy of the available experimental techniques makes determining it experimentally difficult. However, for an RD with multiple crystallographically non-equivalent metastable and saddle-point configurations (as in the case of di-interstitials), the problem becomes practically unsolvable due to its complexity. In this paper, we used a different approach to solving this problem. The molecular dynamics (MD) method was used to calculate the strain dependences of the RD diffusion tensor for various types of strain states. These dependences were used to determine the dipole tensor of the effective RD saddle-point configuration, which takes into account the contributions of all real saddle-point configurations. The proposed approach was used for studying the diffusion characteristics of RDs, such as di-interstitials in FCC copper (used in plasma-facing components of fusion reactors under development). The effect of the external elastic field on the MD-calculated normalized diffusion tensor (ratio of the diffusion tensor to a third of its trace) of di-interstitials was fully consistent with analytical predictions based on the kinetic theory, the parameters of which were the components of the dipole tensors, including the range of non-linear dependence of the diffusion tensor on strains. The results obtained allowed for one to simulate the anisotropic diffusion of di-interstitials in external and internal elastic fields, and to take into account the contribution of di-interstitials to the radiation deformation of crystals. This contribution can be significant, as MD data on the primary radiation damage in copper shows that ~20% of self-interstitial atoms produced by cascades of atomic collisions are combined into di-interstitials.
Existence of Solutions and Hyers–Ulam Stability for a Coupled System of Nonlinear Fractional Differential Equations with p-Laplacian Operator
Authors: Shao Jing , Guo Boling
Published: June 2021

In this paper, the existence and uniqueness of solutions to a coupled formally symmetric system of fractional differential equations with nonlinear p-Laplacian operator and nonlinear fractional differential-integral boundary conditions are obtained by using the matrix eigenvalue method. The Hyers–Ulam stability of the coupled formally symmetric system is also presented with certain growth conditions. By using the topological degree theory and nonlinear functional analysis methods, some sufficient conditions for the existence and uniqueness of solutions to this coupled formally symmetric system are established. Examples are provided to verify our results.
Convergence of Higher Order Jarratt-Type Schemes for Nonlinear Equations from Applied Sciences
Authors: Behl Ramandeep , Argyros Ioannis K. , Mallawi Fouad Othman , Argyros Christopher I.
Published: June 2021

Symmetries are important in studying the dynamics of physical systems which in turn are converted to solve equations. Jarratt’s method and its variants have been used extensively for this purpose. That is why in the present study, a unified local convergence analysis is developed of higher order Jarratt-type schemes for equations given on Banach space. Such schemes have been studied on the multidimensional Euclidean space provided that high order derivatives (not appearing on the schemes) exist. In addition, no errors estimates or results on the uniqueness of the solution that can be computed are given. These problems restrict the applicability of the methods. We address all these problems by using the first order derivative (appearing only on the schemes). Hence, the region of applicability of existing schemes is enlarged. Our technique can be used on other methods due to its generality. Numerical experiments from chemistry and other disciplines of applied sciences complete this study.
Status and Perspectives on Rare Decay Searches in Tellurium Isotopes
Authors: Campani Alice , Dompè Valentina , Fantini Guido
Published: June 2021

Neutrinoless double beta decay (0νββ) is a posited lepton number violating decay whose search is an increasingly active field in modern astroparticle physics. A discovery would imply neutrinos are Majorana particles and inform neutrino physics, cosmology and beyond-standard-model theories. Among the few nuclei where double beta decay (ββ) is allowed, tellurium isotopes stand for their high natural abundance and are currently employed in multiple experiments. The search for 0νββ will provide large exposure data sets in the coming years, paving the way for unprecedented sensitivities. We review the latest rare decay searches in tellurium isotopes and compare past results with theories and prospects from running experiments.
Searching for Gamma-Ray Binaries in Multiwavelength Catalogs
Authors: Martí Josep , Luque-Escamilla Pedro L.
Published: June 2021

The number of gamma-ray binaries currently known is still so small that even a new finding represents a significant expansion of its population. We present a possible hunting strategy based on screening different catalogs to filter potential peculiar stars consistent with gamma-ray sources whose association at lower energies is not yet certain. So far, two candidate systems have emerged from this process; we report about them here.
Case Study of Two Post Vaccination SARS-CoV-2 Infections with P1 Variants in CoronaVac Vaccinees in Brazil
Authors: Estofolete Cassia F. , Banho Cecilia A. , Campos Guilherme R. F. , Marques Beatriz de C. , Sacchetto Livia , Ullmann Leila S. , Possebon Fabio S. , Machado Luana F. , Syrio Juliana D. , Araújo Junior João P. , Bittar Cintia , Rahal Paula , Lobo Suzana M. A. , Ferreira Helena Lage , Vasilakis Nikos , Nogueira Mauricio L.
Published: June 2021

The rapid development of efficacious and safe vaccines against coronavirus disease 2019 (COVID-19) has been instrumental in mitigating the transmission of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Moreover, the emergence of SARS-CoV-2 variants raised concerns on the efficacy of these vaccines. Herein, we report two cases of breakthrough infections with the P1 variant in patients vaccinated with CoronaVac, which is one of the two vaccines authorized for emergency use in the Brazilian immunization program. Our observations suggest that the vaccine reduced the severity of the disease and highlight the potential risk of illness following vaccination and subsequent infection with the P1 variant as well as for continued efforts to prevent and diagnose infection in vaccinated persons.
Human Defensins Inhibit SARS-CoV-2 Infection by Blocking Viral Entry
Authors: Xu Chuan , Wang Annie , Marin Mariana , Honnen William , Ramasamy Santhamani , Porter Edith , Subbian Selvakumar , Pinter Abraham , Melikyan Gregory B. , Lu Wuyuan , Chang Theresa L.
Published: June 2021

Innate immunity during acute infection plays a critical role in the disease severity of severe acute respiratory syndrome (SARS) and Middle East respiratory syndrome (MERS), and is likely to contribute to COVID-19 disease outcomes. Defensins are highly abundant innate immune factors in neutrophils and epithelial cells, including intestinal Paneth cells, and exhibit antimicrobial and immune-modulatory activities. In this study, we investigated the effects of human α- and β-defensins and RC101, a θ-defensin analog, on SARS-CoV-2 infection. We found that human neutrophil peptides (HNPs) 1–3, human defensin (HD) 5 and RC101 exhibited potent antiviral activity against pseudotyped viruses expressing SARS-CoV-2 spike proteins. HNP4 and HD6 had weak anti-SARS-CoV-2 activity, whereas human β-defensins (HBD2, HBD5 and HBD6) had no effect. HNP1, HD5 and RC101 also inhibited infection by replication-competent SARS-CoV-2 viruses and SARS-CoV-2 variants. Pretreatment of cells with HNP1, HD5 or RC101 provided some protection against viral infection. These defensins did not have an effect when provided post-infection, indicating their effect was directed towards viral entry. Indeed, HNP1 inhibited viral fusion but not the binding of the spike receptor-binding domain to hACE2. The anti-SARS-CoV-2 effect of defensins was influenced by the structure of the peptides, as linear unstructured forms of HNP1 and HD5 lost their antiviral function. Pro-HD5, the precursor of HD5, did not block infection by SARS-CoV-2. High virus titers overcame the effect of low levels of HNP1, indicating that defensins act on the virion. HNP1, HD5 and RC101 also blocked viral infection of intestinal and lung epithelial cells. The protective effects of defensins reported here suggest that they may be useful additives to the antivirus arsenal and should be thoroughly studied.
Functional Relevance of the Interaction between Human Cyclins and the Cytomegalovirus-Encoded CDK-Like Protein Kinase pUL97
Authors: Schütz Martin , Steingruber Mirjam , Socher Eileen , Müller Regina , Wagner Sabrina , Kögel Merle , Sticht Heinrich , Marschall Manfred
Published: June 2021

The replication of human cytomegalovirus (HCMV) is characterized by a complex network of virus–host interaction. This involves the regulatory viral protein kinase pUL97, which represents a viral cyclin-dependent kinase ortholog (vCDK) combining typical structural and functional features of host CDKs. Notably, pUL97 interacts with the three human cyclin types T1, H and B1, whereby the binding region of cyclin T1 and the region conferring oligomerization of pUL97 were both assigned to amino acids 231–280. Here, we addressed the question of whether recombinant HCMVs harboring deletions in this region were impaired in cyclin interaction, kinase functionality or viral replication. To this end, recombinant HCMVs were generated by traceless BACmid mutagenesis and were phenotypically characterized using a methodological platform based on qPCR, coimmunoprecipitation, in vitro kinase assay (IVKA), Phos-tag Western blot and confocal imaging analysis. Combined data illustrate the following: (i) infection kinetics of all three recombinant HCMVs, i.e., ORF-UL97 ∆231–255, ∆256–280 and ∆231–280, showed impaired replication efficiency compared to the wild type, amongst which the largest deletion exhibited the most pronounced defect; (ii) specifically, this mutant ∆231–280 showed a loss of interaction with cyclin T1, as demonstrated by CoIP and confocal imaging; (iii) IVKA and Phos-tag analyses revealed strongly affected kinase activity for ∆231–280, with strong impairment of both autophosphorylation and substrate phosphorylation, but less pronounced impairments for ∆231–255 and ∆256–280; and (iv) a bioinformatic assessment of the pUL97–cyclin T1 complex led to the refinement of our current binding model. Thus, the results provide initial evidence for the functional importance of the pUL97–cyclin interaction concerning kinase activity and viral replication fitness.
Diagnosis and Pathogenesis of Nairobi Sheep Disease Orthonairovirus Infections in Sheep and Cattle
Authors: Hartlaub Julia , Gutjahr Benjamin , Fast Christine , Mirazimi Ali , Keller Markus , Groschup Martin H.
Published: June 2021

Nairobi sheep disease orthonairovirus (NSDV) is a zoonotic tick-borne arbovirus, which causes severe gastroenteritis in small ruminants. To date, the virus is prevalent in East Africa and Asia. However, due to climate change, including the spread of transmitting tick vectors and increased animal movements, it is likely that the distribution range of NSDV is enlarging. In this project, sheep and cattle (hitherto classified as resistant to NSDV) were experimentally infected with NSDV for a comparative study of the species-specific pathogenesis. For this purpose, several new diagnostic assays (RT-qPCR, ELISA, iIFA, mVNT, PRNT) were developed, which will also be useful for future epidemiological investigations. All challenged sheep (three different doses groups) developed characteristic clinical signs, transient viremia and virus shedding—almost independent on the applied virus dose. Half of the sheep had to be euthanized due to severe clinical signs, including hemorrhagic diarrhea. In contrast, the course of infection in cattle was only subclinical. However, all ruminants showed seroconversion—implying that, indeed, both species are susceptible for NSDV. Hence, not only sheep but also cattle sera can be included in serological monitoring programs for the surveillance of NSDV occurrence and spread in the future.
From Recoding to Peptides for MHC Class I Immune Display: Enriching Viral Expression, Virus Vulnerability and Virus Evasion
Authors: Atkins John F. , O’Connor Kate M. , Bhatt Pramod R. , Loughran Gary
Published: June 2021

Many viruses, especially RNA viruses, utilize programmed ribosomal frameshifting and/or stop codon readthrough in their expression, and in the decoding of a few a UGA is dynamically redefined to specify selenocysteine. This recoding can effectively increase viral coding capacity and generate a set ratio of products with the same N-terminal domain(s) but different C-terminal domains. Recoding can also be regulatory or generate a product with the non-universal 21st directly encoded amino acid. Selection for translation speed in the expression of many viruses at the expense of fidelity creates host immune defensive opportunities. In contrast to host opportunism, certain viruses, including some persistent viruses, utilize recoding or adventitious frameshifting as part of their strategy to evade an immune response or specific drugs. Several instances of recoding in small intensively studied viruses escaped detection for many years and their identification resolved dilemmas. The fundamental importance of ribosome ratcheting is consistent with the initial strong view of invariant triplet decoding which however did not foresee the possibility of transitory anticodon:codon dissociation. Deep level dynamics and structural understanding of recoding is underway, and a high level structure relevant to the frameshifting required for expression of the SARS CoV-2 genome has just been determined.
Screening and Identification of Lujo Virus Inhibitors Using a Recombinant Reporter Virus Platform
Authors: Welch Stephen R. , Spengler Jessica R. , Genzer Sarah C. , Chatterjee Payel , Flint Mike , Bergeron Éric , Montgomery Joel M. , Nichol Stuart T. , Albariño César G. , Spiropoulou Christina F.
Published: June 2021

Lujo virus (LUJV), a highly pathogenic arenavirus, was first identified in 2008 in Zambia. To aid the identification of effective therapeutics for LUJV, we developed a recombinant reporter virus system, confirming reporter LUJV comparability with wild-type virus and its utility in high-throughput antiviral screening assays. Using this system, we evaluated compounds with known and unknown efficacy against related arenaviruses, with the aim of identifying LUJV-specific and potential new pan-arenavirus antivirals. We identified six compounds demonstrating robust anti-LUJV activity, including several compounds with previously reported activity against other arenaviruses. These data provide critical evidence for developing broad-spectrum antivirals against high-consequence arenaviruses.
A New Dam-Break Outflow-Rate Concept and Its Installation to a Hydro-Morphodynamics Simulation Model Based on FDM (An Example on Amagase Dam of Japan)
Authors: Ahmadi Sayed Masihullah , Yamamoto Yoshimichi
Published: June 2021

Dams are constructed to benefit humans; however, dam-break disasters are unpredictable and inevitable leading to economic and human life losses. The sequential catastrophe of a dam break directly depends on its outflow hydrograph and the extent of population centers that are located downstream of an affected dam. The population density of the cities located in the vicinity of dams has increased in recent times and since a dam break hydrograph relies on many uncertainties and complexities in devising a dam-break outflow hydrograph, more researches for the accurate estimation of a dam-break flood propagation, extent and topography change becomes valuable; therefore, in this paper, the authors propose a novel and simplified dam-break outflow rate equation that is applicable for sudden-partial dam breaks. The proposed equation is extensively affected by a dam-break shape. Therefore, the inference of a dam-break shape on a dam-break outflow rate is investigated in the current study by executing hydraulic experiments in a long, dry bed, frictionless and rectangular water channel connected to a finite water tank to acquire a mean break-shape factor. The proposed equation is further validated by regenerating the Malpasset dam-break hydrograph and comparing it to the existing methods and also by installing it on an existing 2D hydro-morphodynamics flood simulation model. Finally, Amagase Dam’s (arch-reaction dam in Japan) break simulation is executed as a case study. The results of the simulations revealed that the greater the height of a dam-break section, the more devastating its flood consequences would be.
Spatial and Temporal Diversity of Cyanometabolites in the Eutrophic Curonian Lagoon (SE Baltic Sea)
Authors: Pilkaitytė Renata , Overlingė Donata , Gasiūnaitė Zita Rasuolė , Mazur-Marzec Hanna
Published: June 2021

This work aims to determine the profiles of cyanopeptides and anatoxin synthetized by cyanobacteria in the Lithuanian part of the Curonian Lagoon (SE Baltic Sea) and to characterize their spatial and temporal patterns in this ecosystem. Cyanometabolites were analysed by a LC-MS/MS system and were coupled to a hybrid triple quadrupole/linear ion trap mass spectrometer. During the investigation period (2013–2017), 10 microcystins, nodularin, anatoxin-a, 16 anabaenopeptins, including 1 oscillamide, 12 aeruginosins, 1 aeruginosamide, 3 cyanopeptolins and 4 microginins were detected. The most frequently detected metabolites were found at all investigated sites. Demethylated microcystin variants and anabaenopeptins had the strongest relationship with Planktothrix agardhii, while non-demethylated microcystin variants and anatoxin had the strongest relationship with Microcystis spp. Low concentrations of some microcystins: [Asp3]MC-RR, MC-RR, MC-LR, as well as a few other cyanopeptides: AP-A and AEG-A were found during the cold period (December–March). Over the study period, Aphanizomenon, Planktothrix and Microcystis were the main dominant cyanobacteria species, while Planktothrix, Microcystis, and Dolichospermum were potentially producers of cyanopeptides and anatoxin detected in samples from the Curonian Lagoon.
Evaluation of FAO-56 Procedures for Estimating Reference Evapotranspiration Using Missing Climatic Data for a Brazilian Tropical Savanna
Authors: Valle Júnior Luiz Claudio Galvão do , Vourlitis George L. , Curado Leone Francisco Amorim , Palácios Rafael da Silva , Nogueira José de S. , Lobo Francisco de A. , Islam Abu Reza Md Towfiqul , Rodrigues Thiago Rangel
Published: June 2021

The Brazilian savanna (Cerrado) has been heavily impacted by agricultural activities over the last four to five decades, and reliable estimates of reference evapotranspiration (ETo) are needed for water resource management and irrigation agriculture. The Penman–Monteith (PM) is one of the most accepted models for ETo estimation, but it requires many inputs that are not commonly available. Therefore, assessing the FAO guidelines to compute ETo when meteorological data are missing could lead to a better understanding of which variables are critically important for reliable estimates of ETo and how climatic variables are related to water requirements and atmospheric demands. In this study, ETo was computed for a grass-dominated part of the Cerrado from April 2010 to August 2019. We tested 12 different scenarios considering radiation, relative humidity, and/or wind speed as missing climatic data using guidelines given by the FAO. Our results presented that wind speed and actual vapor pressure do not affect ETo estimates as much as the other climatic variables; therefore, in the Cerrado’s conditions, wind speed and relative humidity measurements are less required than temperature and radiation data. When radiation data were missing, the computed ETo was overestimated compared to the benchmark. FAO procedures to estimate the net radiation presented good results during the wet season; however, during the dry season, their results were overestimated because the method could not estimate negative Rn. Our results indicate that radiation data have the highest impact on ETo for our study area and presumably for regions with similar climatic conditions. In addition, those FAO procedures for estimating radiation are not suitable when radiation data are missing.
Analysis of Virtual Water Trade Flow and Driving Factors in the European Union
Authors: Fu Tianbo , Xu Changxin , Huang Xinyi
Published: June 2021

The inefficient application of water resources has become an urgent problem restricting the world’s sustainable development. Virtual Water Trade opens a new perspective on improving water resource utilization efficiency. Based on a multi-regional input–output model and the logarithmic mean Divisia index, the virtual water flows between 2000–2014 in 43 countries and regions have been evaluated, and the driving forces of changes in virtual water flows for the European Union were revealed. During the study period, the total amount of virtual water flow continued to increase. The United Kingdom is a net virtual water importer that depends on the European Union significantly. There was a large amount of virtual water flow from the European Union to the United States during 2000–2012. However, China gradually seized the share of virtual water from European Union exports after 2012. Economic effects and virtual water intensity effects are the most significant drivers of virtual water flows. The difference is that the economic effect positively drives virtual water flows, while the virtual water intensity effect negatively influences. The results reveal the nature of the United Kingdom in the virtual water trade and can provide post-Brexit recommendations.
“One Out–All Out” Principle in the Water Framework Directive 2000—A New Approach with Fuzzy Method on an Example of Greek Lakes
Authors: Latinopoulos Dionissis , Spiliotis Mike , Ntislidou Chrysoula , Kagalou Ifigenia , Bobori Dimitra , Tsiaoussi Vasiliki , Lazaridou Maria
Published: June 2021

The “One Out–All Out” (OOAO) principle imposed by the WFD selects the worst ecological status assessed by different biological quality elements (BQEs). Since it is a precautionary rule that can lead to problems of underestimation of the overall status, its amendment has been a matter of debate for WFD 20+. The use of fuzzy methods that express the functional relationships between variables in ecology and management has been gaining more ground recently. Here is attempted the inclusion of a fuzzy regression among the frequently monitored BQE (phytoplankton) and the outcome of OOAO application in six Greek lakes. The latter was determined by the comparison of four BQE indices in order to assess the extent to which BQEs might underpin the optimal/actual qualitative classification of a waterbody. This approach encompasses the uncertainty and the possibility to broaden the acceptable final EQR based on the character and status of each lake. We concluded that the fuzzy OOAO is an approach that seems to allow a better understanding of the WFD implementation and case-specific evaluation, including the uncertainty in classification as an asset. Moreover, it offers a deeper understanding through self-learning processes based on the existing datasets.
Forensic Investigation of Four Monitored Green Infrastructure Inlets
Authors: Shevade Leena J. , Montalto Franco A.
Published: June 2021

Green infrastructure (GI) is viewed as a sustainable approach to stormwater management that is being rapidly implemented, outpacing the ability of researchers to compare the effectiveness of alternate design configurations. This paper investigated inflow data collected at four GI inlets. The performance of these four GI inlets, all of which were engineered with the same inlet lengths and shapes, was evaluated through field monitoring. A forensic interpretation of the observed inlet performance was conducted using conclusions regarding the role of inlet clogging and inflow rate as described in the previously published work. The mean inlet efficiency (meanPE), which represents the percentage of tributary area runoff that enters the inlet was 65% for the Nashville inlet, while at Happyland the NW inlet averaged 30%, the SW inlet 25%, and the SE inlet 10%, considering all recorded events during the monitoring periods. The analysis suggests that inlet clogging was the main reason for lower inlet efficiency at the SW and NW inlets, while for the SE inlet, performance was compromised by a reverse cross slope of the street. Spatial variability of rainfall, measurement uncertainty, uncertain tributary catchment area, and inlet depression characteristics are also correlated with inlet PE. The research suggests that placement of monitoring sensors should consider low flow conditions and a strategy to measure them. Additional research on the role of various maintenance protocols in inlet hydraulics is recommended.
Efficiency assessment of ion-exchange chromatography as the final stage of anti-VEGF antibody purification
Authors: Mazalyov Denis Alekseevich , Tsivov Aleksey Vladimirovich , Kuvshinov Viktor Andreevich , Zavorueva Mariya Nikolaevna , Smirnov Aleksandr Alekseevich , Eliseeva Vera Vyacheslavovna
Published: June 2021

The article presents the results of chromatographic purification of the anti-VEGF antibody using the ion-exchange sorbent CaptoТМ S and considers the prospects for medical application of the antibody as a therapeutic agent for cancer treatment.
The use of multimodal resins for purification of therapeutic monoclonal antibodies
Authors: Tsivov Aleksey Vladimirovich , Kuvshinov Viktor Andreevich , Mazalyov Denis Alekseevich , Zavorueva Mariya Nikolaevna , Eliseeva Vera Vyacheslavovna , Smirnov Aleksandr Alekseevich
Published: June 2021

Obtaining protein samples with high yield, high purity, and high activity is the foundation for most modern biological studies. Besides, highly purified protein preparations, such as monoclonal antibodies, have been widely used in the treatment of cancer, genetic and orphan diseases.
The development of a method for obtaining strontium-based metal-organic framework materials applicable in bionanotechnology
Authors: Sergorodtseva Antonina Vitalievna , Vodyashkin Andrey Alekseevich , Kalinin Egor Valerievich
Published: June 2021

The work proposes a solvothermal method for obtaining metal-organic framework structures (MOF) based on strontium formate, which have a high sorption capacity and an actively developed porous surface. The effect of the ratio of zinc nitrate to formic acid precursors on the structure, shape and size of MOF has been studied — these properties make it possible to use MOF for biomedical purposes. In addition, studies were carried out to determine the antibacterial properties of the obtained MOF.
Evaluation of the phase solubility of inclusion complexes of diisopropylphenol (INN) with hydroxypropyl-β-cyclodextrin
Authors: Balakhonova Olga Mikhailovna , Tyukova Viktoriya Sergeevna , Kedik Stanislav Anatolievich
Published: June 2021

The paper presents the results of a study of the stability of aqueous solutions of inclusion complexes of hydroxypropyl-β-cyclodextrin with diisopropylphenol in various systems by the Higuchi-Connors phase solubility method. The phase solubility profiles for each system corresponding to the AN type are determined graphically, and the stability constants of the resulting inclusion complexes are calculated. An aqueous solution containing 0.2 % Tween 80 and 0.2 % mannitol was selected as the optimal condition for obtaining the hydroxypropyl-β-cyclodextrin inclusion complex with diisopropylphenol.
Dissolution kinetics profile of the drug «Pimafucin®, vaginal suppositories 100 mg»
Authors: Volkova Aleksandra Mikhailovna , Tyukova Viktoriya Sergeevna , Meleshko Anastasiya Vladimirovna , Kedik Stanislav Anatolievich , Panov Aleksey Valerievich
Published: June 2021

The article presents the dissolution profile and the results of the dissolution test of the drug «Pimafucin®, vaginal suppositories 100 mg.» The samples were examined by UV-spectrophotometry. The relevance of the study is due to the prevalence and availability of this method for quantitative and qualitative analysis of pharmaceutical substances.
The state of pulmonary ventilation with varying degrees of severity of chronic obstructive pulmonary disease caused by tobacco dependence
Authors: Lotkov Vyacheslav Semenovich , Dzyubaylo Anna Vladimirovna
Published: June 2021

The analysis of the ventilation function of the lungs was carried out against the background of different severity of the course of chronic obstructive pulmonary disease, with different severity of nicotine dependence. It was found that an increase in the degree of nicotine dependence is directly proportional to an increase in bronchial resistance and a decrease in the elasticity of lung tissue. The degree of nicotine addiction to a greater extent changes the rate indicators, which increase with treatment.
Analysis of the content of zinc ions in the ejaculate of men with inflammatory diseases of the genitourinary system with indicators of sperm motility
Authors: Gizinger O.A.
Published: June 2021

The results of previous studies have shown that the content of zinc ions in the semen significantly correlated with the number of sperm and influenced the volume of ejaculate. After ejaculation, the zinc contained in the seminal plasma binds to the plasma membrane and stabilizes the sperm DNA. Due to its cyto- and immunoprotective properties: induction of zinc-dependent superoxide dismutase, protection of DNA and transcriptional proteins from free radical oxidation, inhibition of proteinases, zinc is an indispensable trace element in the processes of DNA synthesis and repair, embryogenesis and reproduction.
Mixed herbal products containing polysaccharides pharmacopoeial analysis methods improvement with using pectoral species №1 as example
Authors: Kakhramanova Sabina D. , Dmitry Bokov Dmitry O. , Samylina Irina A.
Published: June 2021

The article discusses pharmacopoeial methods principles for the polysaccharides analysis in crude herbal drugs (CHD) as well as pectoral species No. 1 (coltsfoot leaves 40%, marshmallow roots 40%, oregano herb 20%). Non-pharmacopeial methods are discussed with the rationale for their use for the qualitative and quantitative determination of both individual carbohydrates and the amount of polysaccharides in CHD.
Training of pharmaceutical personnel: the fundamentals and current trends
Authors: Korol L.A. , Zavadskiy S.P.
Published: June 2021

The development of biomedical science in recent decades (a personalized approach to the creation of medicines, the emergence of biotechnological and biosimilar drugs, the transition from empirical to directed drug development using drug design methods - information technologies for their modeling and studying their behavior in biological systems) has a significant impact on the formation of the pharmaceutical education system worldwide. New drugs are being developed such as gene therapeutics and biomedical cell products, and biotechnological production of medicines is developing [1-3]. One of the most important tasks of modern pharmaceutical education is the training of highly qualified personnel in the pharmaceutical industry [4-6]. Global trends in pharmaceutical education are aimed at improving educational programs, introducing new disciplines to the educational process in the light of scientific and technological progress in the industry, developing scientific activities, using digital technologies, interacting actively with industry organizations, and internationalizing education. The state policy of the Russian Federation in the field of medical and pharmaceutical education strives to meet these challenges.
Film coatings in the development of dosage forms
Authors: Shestakov V.N. , Polyakov S.V. , Persanova L.V. , Andreevicheva T. Yu. , Veretennikova M.A. , Ulyanov A.M. , Skupchenko V. V. , Lobov A.V. , Mamedova D.A. , Miranyan K.A. , Dzhafarova T.A.
Published: June 2021

The article provides information about polymers and excipients used for film coating. The main manufacturers, groups of substances used in the compositions, as well as the complexity of the technology and possible defects in the application of coatings, are indicated.
Organic microelement complex in feeding of cows in the production of iodized milk
Authors: Korobov A. P. , Bykova E. V.
Published: June 2021

The optimal level of mineral nutrition of animals is of great importance in the organization of complete feeding. Micromineral feeding of cattle, pigs and poultry has recently received more and more attention. A significant contribution to the solution of this issue is made by an organic microelement complex based on L-aspartic amino acid produced by JSC “Bioamide” under the trademark OMEC-7M. The purpose of the research was to study the effect of the organic microelement complex based on the L-aspartic amino acid OMEC-7M and organic iodine OMEC-J on the milk productivity and composition of cow milk. In order to conduct scientific and economic experiments, groups of cows have been formed according to the principle of analogous pairs taking into account age, live weight, date of the last calving, productivity and physiological state. Live weight of cows was 500–550 kg with a yield of 5000-6000 kg of milk per lactation. The conditions of feeding and housing the animals were the same, with the exception of the studied factors. Studies have been conducted to determine the effect of organic trace elements Cu, Mn, Zn, Fe, Co and Se in the form of asparaginates and organic J on the milk productivity of cows, the composition of milk and the content of iodine in it, the morphological and biochemical composition of blood, the content of the rumen, the digestibility and use of feed nutrients compared to the inorganic salts of these trace elements. As a result of four scientific and economic experiments the effectiveness of the use of the organic microelement complex based on the L-aspartic amino acid OMEC-7M and organic iodine OMEC-J in the feeding of cows in the production of iodized milk has been proved.
Programa de intervención sustentado en el aprendizaje estratégico para orientar el proceso de composición escrita en estudiantes de educación superior
Authors: Alida Flores Carmen
Published: June 2021

El desconocimiento  metacognitivo  que  los estudiantes  del  nivel superior demuestran para componer un texto escrito constituyó el problema central en esta investigación. De allí que se haya propuesto un programa de intervención sustentado en el aprendizaje estratégico para orientar el proceso de composición escrita. La metodología  estuvo  basada  en  un  diseño  de  investigación de campo de carácter descriptivo e interpretativo, apoyada, principalmente, en la metodología cualitativa y, complementada, con el enfoque cuantitativo de investigación. Se aplicó un cuestionario y una entrevista en profundidad a los sujetos de la investigación. Los resultados demostraron  que los estudiantes abordados  solo poseían habilidades lingüísticas de tipo léxico – semántico y sintáctico de su lengua, pero carecían de habilidades estratégicas que les impedían  introducirse  a  niveles profundos  de  la  escritura.  Este hallazgo permitió demostrar la importancia de conocer las debilidades o carencias que poseen los estudiantes a fin de diseñar estrategias adaptadas a sus necesidades
Comprensión lectora de los estudiantes de sexto grado en la Institución Educativa Domingo Savio
Authors: Monge Fierro Winder
Published: June 2021

Estudio que tuvo el propósito de describir la comprensión lectora de los estudiantes de grado sexto de la institución educativa Domingo Savio de San Vicente del Caguán Caquetá-Colombia; ésta se desarrolla mediante una investigación de tipo descriptiva, con un diseño de campo, transeccional contemporáneo univariable. La población la conformaron 77 estudiantes, de grado sexto de la institución estudiada. Para la recolección de los datos se empleó como instrumento un test denominado “Lectura de comprensión lectora” contentiva de 41 ítems; la validez y confiabilidad se realizó mediante la técnica de validación por expertos en ciencias y la confiabilidad se calculó mediante el alfa de Crombach, la cual obtuvo un valor de 0,85 lo que indica que el instrumento es altamente confiable y podía ser aplicado sin ninguna corrección. Los resultados demostraron que los estudiantes medianamente comprenden lo que leen en los niveles literal e inferencial, además, evidencian dificultades para deducir información de los textos; en cuanto que en el nivel crítico no alcanzaron el nivel básico lo cual indica que no lograron un conocimiento global del texto.
crónica mestiza. Características culturales de la latinoamericanidad y la caribeñidad
Authors: Romero Angel María Alexandra
Published: June 2021

Este trabajo tiene como objetivo analizar la obra El entierro de Cortijo de Edgardo Rodríguez Juliá como definición de la latinoamericanidad en la crónica mestiza. La metodología asumida fue la investigación documental. Se realizó el arqueo y la selección de fuentes, su revisión y análisis posterior. Para examinar la obra escogida se aplicó la triple mimesis (prefiguración, configuración y refiguración), para estudiar aspectos vinculados a la crónica, se analizaron obras recientes de este tema y, en relación con el Caribe, se revisaron los estudios de Daroqui y García Canclini. Entre los principales hallazgos se evidenció la influencia de los rasgos históricos y etnográficos del Caribe para configurar el mapeado sociocultural del hombre latinoamericano y caribeño que busca hacerse visible y asumir una posición identitaria dentro del mundo hegemónico opresor. En fin, en el tema de la identidad y el Caribe, la literatura ha sido partícipe de esta definición inacabable.
estructura matricial para abordar la deserción escolar vinculada a la carencia de formación en sexualidad
Authors: Villarroel Garabán Luz Nallidy
Published: June 2021

El presente artículo tiene como objetivo significar una estructura matricial para abordar la deserción escolar vinculada a la carencia de formación en sexualidad en los jóvenes. Metodológicamente se abordó bajo un enfoque cualitativo de tipo descriptivo e interpretativo desde la investigación hermenéutica, tomando para ello informantes claves. La técnica de recolección de la información fue a través de la entrevista a profundidad mediante una pregunta generadora, aunado a ello la observación participante y el cuaderno del investigador. Asimismo, los datos obtenidos de las diferentes fuentes se trataron bajo un proceso de categorización, codificación y triangulación. Luego de recorrer esta realidad se procedió al análisis e interpretación de los hallazgos que conllevaron generar la estructura matricial, aunado a un gran aporte teórico, mediante el cual se espera que al poner en práctica, permitirá disminuir los casos de deserción escolar vinculada a la carencia de formación en sexualidad en los jóvenes.
Desarrollo de valores sociocomunitarios con estrategias dinámicas para vivir bien
Authors: Cabezas Veizan Erlinda , Siñani Alaro Edwin Saúl
Published: June 2021

Investigación educativa cuyo objetivo fue analizar el desarrollo de la dimensión del Ser, principalmente en el valor de la responsabilidad con estrategias lúdicas. Básicamente se fundamentó en referencias teóricas provenientes de documentos emanados del Ministerio de Educación del Estado Plurinacional de Bolivia. La metodología se sustentó en el paradigma cualitativo de tipo descriptivo, con enfoque hermenéutico de la acción pedagógica transformadora. Mediante la aplicación de las diferentes estrategias fue posible desarrollar la dimensión del Ser, tomando en cuenta el aporte del modelo que está orientado a la obtención de la teoría a partir de la práctica. Los resultados procuraron una mejor comprensión de los elementos del Modelo Educativo Sociocomunitario Productivo (MESCP), puntualidad, participación activa de padres de familia y mayor cumplimiento de las tareas. Se concluyó que con el abordaje metodológico se logró evaluar de un modo más integral en las cuatro dimensiones del ser humano: Ser, Saber, Hacer y Decidir; además de la implementación de la autoevaluación, la cual fue adecuada a niños en etapa de aprendizaje de la lectura y escritura.