bluuebunny/crossref_metadata_2025 huggingface dataset:
Assessment of Mechanism of Pore Formation in Directionally Solidified A356 Alloy
Authors: Uludağ M. , Dişpinar D.
Published: March 2017

It is well-known that the better the control of the liquid aluminium allows obtaining of better properties. One of the most important defects that is held responsible for lower properties has been the presence of porosity. Porosity has always been associated with the amount of dissolved hydrogen in the liquid. However, it was shown that hydrogen was not the major source but only a contributor the porosity. The most important defect that causes porosity is the presence of bifilms. These defects are surface entrained mainly due to turbulence and uncontrolled melt transfer. In this work, a cylindrical mould was designed (Ø30 x 300 mm) both from sand and die. Moulds were produced both from sand and die. Water cooled copper chill was placed at the bottom of the mould in order to generate a directional solidification. After the melt was prepared, prior to casting of the DC cast samples, reduced pressure test sample was taken to measure the melt quality (i.e. bifilm index). The cast parts were then sectioned into regions and longitudinal and transverse areas were investigated metallographically. Pore size, shape and distribution was measured by image analysis. The formation of porosity was evaluated by means of bifilm content, size and distribution in A356 alloy.
Application of FEM Analysis for Evaluation of the Stress and Deformation Distributions in the Top Layer of A390.0 Alloy During Friction
Authors: Wieszała R. , Piątkowski J. , Bąkowski H.
Published: March 2017

The paper presents an analysis of the effect of shape of primary silicon crystals on the sizes of stresses and deformations in a surface layer of A390.0 alloy by Finite Elements Method (FEM). Analysis of stereological characteristics of the studied alloy, performed based on a quantitative metallographic analysis in combination with a statistical analysis, was used for this purpose. The presented simulation tests showed not only the deposition depth of maximum stresses and strains, but also allowed for determining the aforementioned values depending on the shape of the silicon crystals. The studied material is intended for pistons of internal combustion engines, therefore the analysis of the surface layer corresponded to conditions during friction in a piston-cylinder system of an internal combustion engine having power of up to 100 kW. The obtained results showed important differences in the values of stresses and strains up to 15% between various shape of the silicon crystals. Crystals with sharp edges caused higher stresses and deformation locally than those with rounded shapes.
Sound Intensity Distribution Around Organ Pipe
Authors: Odya Piotr , Kotus Józef , Szczodrak Maciej , Kostek Bożena
Published: March 2017

The aim of the paper was to compare acoustic field around the open and stopped organ pipes. The wooden organ pipe was located in the anechoic chamber and activated with a constant air flow, produced by an external air-compressor. Thus, a long-term steady state response was possible to obtain. Multi-channel acoustic vector sensor was used to measure the sound intensity distribution of radiated acoustic energy. Measurements have been carried out on a defined fixed grid of points. A specialized Cartesian robot allowed for a precise positioning of the acoustic probe. The resulted data were processed in order to obtain and visualize the sound intensity distribution around the pipe, taking into account the type of the organ pipe, frequency of the generated sound, the sound pressure level and the direction of acoustic energy propagation. For the open pipe, an additional sound source was identified at the top of the pipe. In this case, the streamlines in front of the pipe are propagated horizontally and in a greater distance than in a case of the stopped pipe, moreover they are directed downwards. For the stopped pipe, the streamlines of the acoustic flow were directed upwards. The results for both pipe types were compared and discussed in the paper.
Hearing Status in Young People Using Portable Audio Players
Authors: Pawlaczyk-Łuszczyńska Małgorzata , Zaborowski Kamil , Zamojska-Daniszewska Małgorzata , Rutkowska-Kaczmarek Paulina , Dudarewicz Adam , Śliwinska-Kowalska Mariola
Published: March 2017

The aim of this study was to evaluate the hearing status in young adults using portable audio players (PAPs) in relation to their listening habits.

The study included 58 subjects, aged 22.8±2.8 years, non-occupationally exposed to noise. Questionnaire inquiry aimed at collecting personal data, the information on PAPs usage habits, self-assessment of hearing status and identification of risk factors for noise-induced hearing loss (NIHL) were performed in study subjects. Hearing tests included pure-tone audiometry (PTA) and transient-evoked otoacoustic emission (TEOAE).

All subjects were PAPs users. Depending on listening habits they were divided into the subgroups of “frequent” users (>1 h/day) and “non-frequent” users (≤1 h/day). There were no significant differences between subgroups in prevalence of NIHL risk factors and self-assessment of hearing status. However, frequent users more often complained of tinnitus and hyperacusis.

Majority (81.9%) of participants had normal hearing. Nevertheless, 6.9% of audiograms showed high- frequency notches typical for NIHL. Both, the PTA and TEOAE indicated worse hearing in non-frequent users compared to frequent users. No significant differences in prevalence of high-frequency notches be- tween subgroups were noted.

The outcomes do not support some previous studies results that the excessive exposure to music listened through PAPs might result in accelerating of development of NIHL loss in young adults.
Speaker Model Clustering to Construct Background Models for Speaker Verification
Authors: Dişken Gökay , Tüfekci Zekeriya , Çevik Ulus
Published: March 2017

Conventional speaker recognition systems use the Universal Background Model (UBM) as an imposter for all speakers. In this paper, speaker models are clustered to obtain better imposter model representations for speaker verification purpose. First, a UBM is trained, and speaker models are adapted from the UBM. Then, the k-means algorithm with the Euclidean distance measure is applied to the speaker models. The speakers are divided into two, three, four, and five clusters. The resulting cluster centers are used as background models of their respective speakers. Experiments showed that the proposed method consistently produced lower Equal Error Rates (EER) than the conventional UBM approach for 3, 10, and 30 seconds long test utterances, and also for channel mismatch conditions. The proposed method is also compared with the i-vector approach. The three-cluster model achieved the best performance with a 12.4% relative EER reduction in average, compared to the i-vector method. Statistical significance of the results are also given.
Hydrothermal green synthesis of gold nanoparticles using mushroom (Agaricus bisporus) extract: physico-chemical characteristics and antifungal activity studies
Authors: Eskandari-Nojedehi Maryam , Jafarizadeh-Malmiri Hoda , Rahbar-Shahrouzi Javad
Published: March 2017

Edible mushroom (Agaricus bisporus) extract was used to synthesize gold nanoparticles (AuNPs) through hydrothermal process (at a pressure of 15 psi and a temperature of 121°C for 15 min). Response surface methodology was applied to monitor the influence of the synthesis parameters, namely: the mushroom extract concentration (1–9 gr DP/100 ml distilled water) and the amount of HAuCL4·3H2O solution (8–12 ml) on the particle size and concentration of fabricated AuNPs. The obtained results demonstrated that while the main and quadratic terms of the synthesis parameters had significant (p<0.05) effects on the response variables, their interactions had insignificant effect on them. The results indicated that spherical synthesized AuNPs using 10 ml of HAuCl4·3H2O solution (1 mm) and 1 ml of mushroom extract with concentration of 5 gr DP/100 ml had mean particle size (25 nm) and maximum concentration (534 ppm) and stability (zeta potential of –45.8 mV). The results revealed that mushroom extract could act as both reducing and stabilizing agents due to its bioactive compounds. Fourier-transform infrared analysis showed that polyols and carbonyl groups in mushroom extract had strong effects on formation of stable AuNPs. The fabricated AuNPs exhibited high antifungal activity againstAspergillus flavusas compared to theAspergillus terreus.
Aus „Information und Dokumentation“ wird „Informations- und Datenmanagement“ – zur Neuausrichtung des dokumentarischen Studiengangs an der FH Potsdam
Authors: Däßler Rolf , Schreyer Angela
Published: March 2017

Die digitale Gesellschaft und der stetige technologische Wandel erfordern eine kontinuierliche Anpassung der Curricula informationswissenschaftlicher Studiengänge. Die Fachhochschule Potsdam bietet ab dem Wintersemester 2016/2017 den neu ausgerichteten und für sieben Jahre akkreditierten Bachelor-Studiengang „Informations- und Datenmanagement“ an, der ein informationswissenschaftliches Grundlagenstudium mit neuen Ausbildungsinhalten vor allem in den Bereichen Modellierung und automatisierte Verfahren verbindet. Die thematischen Schwerpunkte des methoden- und anwendungsorientierten Studiums sind dabei Datenmanagement, Informationsmanagement, Wissensmanagement und Information Retrieval.
Evaluation of corrosion resistance of powder coatings after various surface chemical pre-treatment
Authors: Draganovská D. , Brezinová J.
Published: March 2017

The surface treatment by a powder coating is one of the progressive technologies. Such coatings are resistant to corrosion and mechanical wearing. The quality of surface protection is affected primarily by a layer pre-treatment, the type of surface tretment, the system selection and the method and quality of application. Taking into account all the surface pre-tretment methods, the chemical pre-modification is a leading method. One of the methods is pre-treatment using a conversion coating which was developed on the nanotechnology basis - BONDRITE NT. That non-phosphate chemical pre-treatment is utilized at a surrounding temperature. It creates nano-ceramic protective layer on steel, zinc and aluminium surfaces, and as the result the coating has a significant adhesion. In the paper, the possibilities for the improvement of corrosion resistance of powder coatings using the subject conversion coating are presented at the current pasivation of respective surface.
A new view of relationship between atomic posets and complete (algebraic) lattices
Authors: Yu Bin , Li Qingguo , Wu Huanrong
Published: March 2017

In the context of the atomic poset, we propose several new methods of constructing the complete lattice and the algebraic lattice, and the mutual decision of relationship between atomic posets and complete lattices (algebraic lattices) is studied.
Effects of Specific Fuel Consumption and Exhaust Emissions of Four Stroke Diesel Engine with CuO/Water Nanofluid as Coolant
Authors: Senthilraja S. , Vijayakumar KCK. , Gangadevi R.
Published: March 2017

This article reports the effects of CuO/water based coolant on specific fuel consumption and exhaust emissions of four stroke single cylinder diesel engine. The CuO nanoparticles of 27 nm were used to prepare the nanofluid-based engine coolant. Three different volume concentrations (i.e 0.05%, 0.1%, and 0.2%) of CuO/water nanofluids were prepared by using two-step method. The purpose of this study is to investigate the exhaust emissions (NOx), exhaust gas temperature and specific fuel consumption under different load conditions with CuO/water nanofluid. After a series of experiments, it was observed that the CuO/water nanofluids, even at low volume concentrations, have a significant influence on exhaust emissions. The experimental results revealed that, at full load condition, the specific fuel consumption was reduced by 8.6%, 15.1% and 21.1% for the addition of 0.05%, 0.1% and 0.2% CuO nanoparticles with water, respectively. Also, the emission tests were concluded that 881 ppm, 853 ppm and 833 ppm of NOx emissions were observed at high load with 0.05%, 0.1% and 0.2% volume concentrations of CuO/water nanofluids, respectively.
An Improved XFEM for the Poisson Equation with Discontinuous Coefficients
Authors: Stąpór Paweł
Published: March 2017

Discontinuous coefficients in the Poisson equation lead to the weak discontinuity in the solution, e.g. the gradient in the field quantity exhibits a rapid change across an interface. In the real world, discontinuities are frequently found (cracks, material interfaces, voids, phase-change phenomena) and their mathematical model can be represented by Poisson type equation. In this study, the extended finite element method (XFEM) is used to solve the formulated discontinuous problem. The XFEM solution introduce the discontinuity through nodal enrichment function, and controls it by additional degrees of freedom. This allows one to make the finite element mesh independent of discontinuity location. The quality of the solution depends mainly on the assumed enrichment basis functions. In the paper, a new set of enrichments are proposed in the solution of the Poisson equation with discontinuous coefficients. The global and local error estimates are used in order to assess the quality of the solution. The stability of the solution is investigated using the condition number of the stiffness matrix. The solutions obtained with standard and new enrichment functions are compared and discussed.
Estimation of Conditional Expected Value for Exponentially Autocorrelated Data
Authors: Kowalczyk Adam , Szlachta Anna , Hanus Robert , Chorzępa Rafał
Published: March 2017

Autocorrelation of signals and measurement data makes it difficult to estimate their statistical characteristics. However, the scope of usefulness of autocorrelation functions for statistical description of signal relation is narrowed down to linear processing models. The use of the conditional expected value opens new possibilities in the description of interdependence of stochastic signals for linear and non-linear models. It is described with relatively simple mathematical models with corresponding simple algorithms of their practical implementation.

The paper presents a practical model of exponential autocorrelation of measurement data and a theoretical analysis of its impact on the process of conditional averaging of data. Optimization conditions of the process were determined to decrease the variance of a characteristic of the conditional expected value. The obtained theoretical relations were compared with some examples of the experimental results.
Estimation of UAV Position with Use of Smoothing Algorithms
Authors: Kaniewski Piotr , Gil Rafał , Konatowski Stanisław
Published: March 2017

The paper presents methods of on-line and off-line estimation of UAV position on the basis of measurements from its integrated navigation system. The navigation system installed on board UAV contains an INS and a GNSS receiver. The UAV position, as well as its velocity and orientation are estimated with the use of smoothing algorithms. For off-line estimation, a fixed-interval smoothing algorithm has been applied. On-line estimation has been accomplished with the use of a fixed-lag smoothing algorithm. The paper includes chosen results of simulations demonstrating improvements of accuracy of UAV position estimation with the use of smoothing algorithms in comparison with the use of a Kalman filter.
Programmable Input Mode Instrumentation Amplifier Using Multiple Output Current Conveyors
Authors: Pankiewicz Bogdan
Published: March 2017

In this paper a programmable input mode instrumentation amplifier (IA) utilising second generation, multiple output current conveyors and transmission gates is presented. Its main advantage is the ability to choose a voltage or current mode of inputs by setting the voltage of two configuration nodes. The presented IA is prepared as an integrated circuit block to be used alone or as a sub-block in a microcontroller or in a field programmable gate array (FPGA), which shall condition analogue signals to be next converted by an analogue-to-digital converter (ADC). IA is designed in AMS 0.35 µm CMOS technology and the power supply is 3.3 V; the power consumption is approximately 9.1 mW. A linear input range in the voltage mode reaches ± 1.68 V or ± 250 µA in current mode. A passband of the IA is above 11 MHz. The amplifier works in class A, so its current supply is almost constant and does not cause noise disturbing nearby working precision analogue circuits.
Systematics and faunistics of Neotropical Eucosmini, 4: three new genera and their species (Lepidoptera: Tortricidae)
Authors: Razowski Józef , Becker Vitor Osmar
Published: March 2017

Three genera (Brasilnotia gen. n., Malinova gen. n., Tiburica gen. n.) and three species (Brasilnotia planaltinana sp. n., Malinova novaelimae sp. n., Tiburica paranae sp. n.) from Brazil are described and illustrated.
First record of Phytodietus moragai Gauld, 1997 (Hymenoptera: Ichneumonidae: Tryphoninae) from Ecuador, with a description of the female of this species
Authors: Kostro-Ambroziak Agata , Di Giovanni Filippo
Published: March 2017

Phytodietus moragai Gauld, 1997, previously known from Costa Rica, is recorded from Ecuador for the first time. This is the second representative of the genus Phytodietus Gravenhorst, 1829 from South America. A description and illustration of the female of P. moragai is provided.
Granuloma Annulare-like Wells Syndrome in a Child - A Case Report
Authors: Lekić Branislav , Gajić-Veljić Mirjana , Nikolić Miloš
Published: March 2017

Wells syndrome (WS) is a rare inflammatory skin disease of unknown etiology. Possible triggers for WS include insect bites/stings, infections, medications, malignancies, and vaccination. Most cases have been reported in adults, but WS may also occur in children.

We report a case of idiopathic WS in a 12-year-old boy, who presented with pruritic papulonodular and granuloma annulare-like lesions on his legs. The patient had an excellent response to topical and systemic corticosteroids.

WS may present as plaque, granuloma annulare-like, urticaria-like, papulovesicular, bullous, papulonodular, or fixed drug eruption-like lesions. Erythematous annular lesions are most common in adults, while plaques are mostly found in children. The histopathologic features are dynamic, starting with dermal edema and infiltration of eosinophils, then flame figures develop, and finishing with the appearance of histiocytes and giant cells.

Our patient represents a rare pediatric case with granuloma annulare-like WS syndrome.
Dermoscopy of difficult-to-diagnose Melanomas
Authors: Papageorgiou Chrysoula , Ioannides Demetrios , Apalla Zoe , Vakirlis Efstratios , Lazaridou Elisabeth , Sotiriou Eleni , Lallas Aimilios
Published: March 2017

Dermoscopy is a non-invasive procedure that allows the evaluation of cutaneous lesions, and is considered to be a useful tool that improves the diagnostic accuracy of melanoma. Many dermoscopic criteria of melanoma have been established and several algorithms have been created for melanoma detection. However, the recognition of some melanomas remains challenging. Melanomas on specific body sites, melanomas in patients with multiple atypical moles, and nodular melanomas represent the most difficult-to-recognize melanoma subtypes, since they typically lack the “classic” melanoma-specific criteria. This paper provides an update on dermoscopy of difficult-to-diagnose melanomas by summarizing the newest data. Lastly, we highlight the importance of digital dermoscopy in the follow-up of melanocytic lesions for the detection of incipient melanomas while maintaining a low excision rate.
The Determination of Some Pollution Parameters, Water Quality and Heavy Metal Concentrations of Aci Lake (Karapinar/Konya, Turkey)
Authors: Akköz Cengiz
Published: March 2017

Water quality parameters were measured in Acı Lake. The measurements were carried out in water samples taken from five different stations over ten months. According to the results of the analyses done on the samples of water, without taking into consideration the differences of seasons and stations, the average annual values of heavy metal concentrations for Cr, Cu and Ni were found to be 0.10 mg/l, 0.11 mg/l and 0.19 mg/l dry weight, respectively. Surprisingly, Co and Cd metals were not detected at any station or at any season. Other water quality parameters, namely pH, turbidity and nitrate, fall in the intermediate value range for these measures. The comparisons of the measured parameters and the metal concentration with the water quality index show that the water quality of Acı Lake prevents the water being used as irrigation water due to high salt levels.
Sleep Deprivation and Caffeine Treatment Potentiate Photic Resetting of the Master Circadian Clock in a Diurnal Rodent
Authors: Jha Pawan Kumar , Bouâouda Hanan , Gourmelen Sylviane , Dumont Stephanie , Fuchs Fanny , Goumon Yannick , Bourgin Patrice , Kalsbeek Andries , Challet Etienne
Published: March 2017

Circadian rhythms in nocturnal and diurnal mammals are primarily synchronized to local time by the light/dark cycle. However, nonphotic factors, such as behavioral arousal and metabolic cues, can also phase shift the master clock in the suprachiasmatic nuclei (SCNs) and/or reduce the synchronizing effects of light in nocturnal rodents. In diurnal rodents, the role of arousal or insufficient sleep in these functions is still poorly understood. In the present study, diurnal Sudanian grass rats,Arvicanthis ansorgei, were aroused at night by sleep deprivation (gentle handling) or caffeine treatment that both prevented sleep. Phase shifts of locomotor activity were analyzed in grass rats transferred from a light/dark cycle to constant darkness and aroused in early night or late night. Early night, but not late night, sleep deprivation induced a significant phase shift. Caffeine on its own induced no phase shifts. Both sleep deprivation and caffeine treatment potentiated light-induced phase delays and phase advances in response to a 30 min light pulse, respectively. Sleep deprivation in early night, but not late night, potentiated light-induced c-Fos expression in the ventral SCN. Caffeine treatment in midnight triggered c-Fos expression in dorsal SCN. Both sleep deprivation and caffeine treatment potentiated light-induced c-Fos expression in calbindin-containing cells of the ventral SCN in early and late night. These findings indicate that, in contrast to nocturnal rodents, behavioral arousal induced either by sleep deprivation or caffeine during the sleeping period potentiates light resetting of the master circadian clock in diurnal rodents, and activation of calbindin-containing suprachiasmatic cells may be involved in this effect.

SIGNIFICANCE STATEMENTArousing stimuli have the ability to regulate circadian rhythms in mammals. Behavioral arousal in the sleeping period phase shifts the master clock in the suprachiasmatic nuclei and/or slows down the photic entrainment in nocturnal animals. How these stimuli act in diurnal species remains to be established. Our study in a diurnal rodent, the Grass rat, indicates that sleep deprivation in the early rest period induces phase delays of circadian locomotor activity rhythm. Contrary to nocturnal rodents, both sleep deprivation and caffeine-induced arousal potentiate the photic entrainment in a diurnal rodent. Such enhanced light-induced circadian responses could be relevant for developing chronotherapeutic strategies.
Rivoluzione oligarchica o restaurazione della democrazia?
Authors: Marcaccini Carlo
Published: March 2017

The number of the Five Thousand, established during the so-called oligarchic revolution in 411, could result from the procedure approved in 487/6 under the archonship of Telesinos (κλήρωσις ἐκ προκρίτων). The selection of the Five Thousand could be similar to the πρόκρισις of the candidates for the offices in the early fifth century. This could prove the hypothesis of de Ste. Croix: for him the Five Thousand had only the power to hold the offices and the teti were not excluded from the ekklesia. The patrioi nomoi of Cleisthenes, which, according to the Clitophon’s rider, had to be examined in order to modify the constitution, could have included the selection of five thousand candidates for the offices. Thus, the issue of the patrios politeia would not be mere propaganda, but could also imply actual provisions. The Five Thousand were not an oligarchy but a moderate democracy.
Louis Sullivan and the Physiognomic Translation of American Character
Authors: Davis Charles L.
Published: March 2017

Louis Sullivan and the Physiognomic Translation of American Character examines the racial politics of Louis Sullivan's democratic vision for American architecture, as manifest in his interpretations of physiognomic character in people and the built environment and in his reflections on U.S. nationalism. Charles L. Davis II argues that while Sullivan believed that ordinary Americans would produce an indigenous culture reflective of democratic ideals, his assimilationist conception of American citizenship excluded recent white immigrants and resident nonwhite peoples and limited his democratic architecture, as in the case of Kehilath Anshe Ma'ariv Synagogue in Chicago. While Sullivan's ornament for the synagogue expressed Jewish identity in Chicago, its Richardsonian exterior referred to his secular-assimilationist model of national culture. The synagogue's subsequent use as Pilgrim Baptist Church by an African American congregation complicates our understanding of Sullivan's assimilationist political theory and its expression in his architecture.
A covalently bound inhibitor triggers EZH2 degradation through CHIP‐mediated ubiquitination
Authors: Wang Xu , Cao Wei , Zhang Jianjun , Yan Ming , Xu Qin , Wu Xiangbing , Wan Lixin , Zhang Zhiyuan , Zhang Chenping , Qin Xing , Xiao Meng , Ye Dongxia , Liu Yuyang , Han Zeguang , Wang Shaomeng , Mao Li , Wei Wenyi , Chen Wantao
Published: March 2017

Enhancer of zeste homolog 2 (EZH2) has been characterized as a critical oncogene and a promising drug target in human malignant tumors. The current EZH2 inhibitors strongly suppress the enhanced enzymatic function of mutant EZH2 in some lymphomas. However, the recent identification of a PRC2‐ and methyltransferase‐independent role of EZH2 indicates that a complete suppression of all oncogenic functions of EZH2 is needed. Here, we report a unique EZH2‐targeting strategy by identifying a gambogenic acid (GNA) derivative as a novel agent that specifically and covalently bound to Cys668 within the EZH2‐SET domain, triggering EZH2 degradation through COOH terminus of Hsp70‐interacting protein (CHIP)‐mediated ubiquitination. This class of inhibitors significantly suppressed H3K27Me3 and effectively reactivated polycomb repressor complex 2 (PRC2)‐silenced tumor suppressor genes. Moreover, the novel inhibitors significantly suppressed tumor growth in an EZH2‐dependent manner, and tumors bearing a non‐GNA‐interacting C668S‐EZH2 mutation exhibited resistance to the inhibitors. Together, our results identify the inhibition of the signaling pathway that governs GNA‐mediated destruction of EZH2 as a promising anti‐cancer strategy.
The retinoblastoma homolog RBR1 mediates localization of the repair protein RAD51 to DNA lesions in Arabidopsis
Authors: Biedermann Sascha , Harashima Hirofumi , Chen Poyu , Heese Maren , Bouyer Daniel , Sofroni Kostika , Schnittger Arp
Published: March 2017

The retinoblastoma protein (Rb), which typically functions as a transcriptional repressor of E2F‐regulated genes, represents a major control hub of the cell cycle. Here, we show that loss of the Arabidopsis Rb homolog RETINOBLASTOMA‐RELATED 1 (RBR1) leads to cell death, especially upon exposure to genotoxic drugs such as the environmental toxin aluminum. While cell death can be suppressed by reduced cell‐proliferation rates, rbr1 mutant cells exhibit elevated levels of DNA lesions, indicating a direct role of RBR1 in the DNA‐damage response (DDR). Consistent with its role as a transcriptional repressor, we find that RBR1 directly binds to and represses key DDR genes such as RADIATION SENSITIVE 51 (RAD51), leaving it unclear why rbr1 mutants are hypersensitive to DNA damage. However, we find that RBR1 is also required for RAD51 localization to DNA lesions. We further show that RBR1 is itself targeted to DNA break sites in a CDKB1 activity‐dependent manner and partially co‐localizes with RAD51 at damage sites. Taken together, these results implicate RBR1 in the assembly of DNA‐bound repair complexes, in addition to its canonical function as a transcriptional regulator.
Hypoxia enhances antibody‐dependent dengue virus infection
Authors: Gan Esther Shuyi , Cheong Wei Fun , Chan Kuan Rong , Ong Eugenia Ziying , Chai Xiaoran , Tan Hwee Cheng , Ghosh Sujoy , Wenk Markus R , Ooi Eng Eong
Published: March 2017

Dengue virus (DENV) has been found to replicate in lymphoid organs such as the lymph nodes, spleen, and liver in post‐mortem analysis. These organs are known to have low oxygen levels (~0.5–4.5% O2) due to the vascular anatomy. However, how physiologically low levels of oxygen affect DENV infection via hypoxia‐induced changes in the immune response remains unknown. Here, we show that monocytes adapted to 3% O2 show greater susceptibility to antibody‐dependent enhancement of DENV infection. Low oxygen level induces HIF1α‐dependent upregulation of fragment crystallizable gamma receptor IIA (FcγRIIA) as well as HIF1α‐independent alterations in membrane ether lipid concentrations. The increased FcγRIIA expression operates synergistically with altered membrane composition, possibly through increase membrane fluidity, to increase uptake of DENV immune complexes for enhanced infection. Our findings thus indicate that the increased viral burden associated with secondary DENV infection is antibody‐dependent but hypoxia‐induced and suggest a role for targeting hypoxia‐induced factors for anti‐dengue therapy.
Development of LC3/GABARAP sensors containing a LIR and a hydrophobic domain to monitor autophagy
Authors: Lee You‐Kyung , Jun Yong‐Woo , Choi Ha‐Eun , Huh Yang Hoon , Kaang Bong‐Kiun , Jang Deok‐Jin , Lee Jin‐A
Published: March 2017

Macroautophagy allows for bulk degradation of cytosolic components in lysosomes. Overexpression of GFP/RFP‐LC3/GABARAP is commonly used to monitor autophagosomes, a hallmark of autophagy, despite artifacts related to their overexpression. Here, we developed new sensors that detect endogenous LC3/GABARAP proteins at the autophagosome using an LC3‐interacting region (LIR) and a short hydrophobic domain (HyD). Among HyD‐LIR‐GFP sensors harboring LIR motifs of 34 known LC3‐binding proteins, HyD‐LIR(TP)‐GFP using the LIR motif from TP53INP2 allowed detection of all LC3/GABARAPs‐positive autophagosomes. However, HyD‐LIR(TP)‐GFP preferentially localized to GABARAP/GABARAPL1‐positive autophagosomes in a LIR‐dependent manner. In contrast, HyD‐LIR(Fy)‐GFP using the LIR motif from FYCO1 specifically detected LC3A/B‐positive autophagosomes. HyD‐LIR(TP)‐GFP and HyD‐LIR(Fy)‐GFP efficiently localized to autophagosomes in the presence of endogenous LC3/GABARAP levels and without affecting autophagic flux. Both sensors also efficiently localized to MitoTracker‐positive damaged mitochondria upon mitophagy induction. HyD‐LIR(TP)‐GFP allowed live‐imaging of dynamic autophagosomes upon autophagy induction. These novel autophagosome sensors can thus be widely used in autophagy research.
Caracterización do xénero fantástico propio da Literatura Infantil e Xuvenil
Authors: García Pedreira Rocío
Published: March 2017

Na actualidade, aínda se fai difícil concretar o alcance do termo literatura fantástica. Por outra banda, o mesmo apelativo fai referencia a obras moi diferenciadas no caso da literatura para adultos e no da literatura para os máis novos. A presencia do fantástico neste último grupo ten unha orixe distinta, e significa para os seus receptores a posibilidade de entrar en contacto con lecturas que reflicten a súa forma de entender o mundo. No presente traballo argumentarase a necesidade e validez do fantástico na Literatura Infantil e Xuvenil ao mesmo tempo que se determinarán as formas que adopta o xénero ao que fai referencia.
Priešoperacinių veiksnių įtaka prognozuojant kūno masės sumažėjimą praėjus penkeriems metams po nutukimo gydymo skrandžio apjuosimo reguliuojama juosta operacijos: perspektyvusis atsitiktinių imčių tyrimas
Authors: Juodeikis Žygimantas , Brimas Gintautas
Published: March 2017

Įvadas Skrandžio apjuosimo reguliuojama juosta operacija klinikinėje praktikoje atliekama daugiau nei 30 metų. Jos efektyvumą patvirtino daugelis studijų, tačiau nepakanka tyrimų, kuriuose būtų analizuojami priešoperaciniai kūno masės pokyčių prog­nostiniai veiksniai.Tikslas Nustatyti priešoperacinius prognostinius veiksnius, palyginant dvi skirtingas skrandį apjuosiančias reguliuojamas juostas (SAGB ir Minimizer Extra), siekiant geresnių kūno masės mažėjimo rezultatų.Ligoniai ir metodai Nuo 2009 m. sausio 1 d. iki 2010 m. sausio 31 d. į tyrimą buvo įtraukti 103 morbidiniu nutukimu sergantys ligoniai. SAGB juosta naudota 49 ligoniams, MiniMizer Extra – 54 pacientams. Ligoniai pakartotinai ištirti praėjus 1 ir 5 metams. Iš veiksnių, koreliuojančių su procentiniu perteklinės kūno masės sumažėjimu (%PKMS), sudarėme prognostinius regresijos modelius.Rezultatai Vidutinis ligonių amžius buvo 46,1 ± 11,5 metų. Vidutinis kūno masės indeksas (KMI) – 47,5 ± 7,3 kg/m2. Po 5 metų pakarto­tinai ištirta 90 (87,3%) ligonių. Vidutinis %PKMS po 5 metų buvo 47,3 ± 29,7%, lyginant SAGB ir MiniMizer juostas statistiškai reikšmingo skirtumo (44,1 ± 31,9 ir 50,3 ± 27,6; p = 0,14) nebuvo. Pacientų amžius, priešoperacinė juosmens apimtis ir KMI statistiškai reikšmingai koreliavo su %PKMS.Lyginant SAGB ir Minimizer juostas, po 1 metų %PKMS buvo statistiškai reikšmingai didesnis mažiau nutukusių (KMI ≤ 47 kg/m2) MiniMizer Extra pacientų grupėje. Praėjus 1 ir 5 metams, %PKMS buvo statistiškai reikšmingai didesnis vyresnių nei 40 metų MiniMizer Extra ligonių grupėje.Išvados Didesnis pooperacinis %PKMS gali būti pasiektas vyresniems nei 40 metų pacientams, skrandžio apjuosimo reguliuojama juosta operacijai naudojant MiniMizer Extra juostą. Sudaryti prognostiniai modeliai leidžia numatyti %PKMS naudojant SAGB ir MiniMizer juostas.
Jatrogeninis trečio laipsnio krūties nušalimas: klinikinis atvejis
Authors: Čepas Adas , Rimdeika Rytis , Aštrauskaitė Goda , Žurman Elina
Published: March 2017

Jatrogeniniai nušalimai yra ypač reti medicinos praktikoje. Remiantis pasaulinės literatūros duomenimis, yra užfiksuotas tik vienas konservatyviai gydytas jatrogeninio krūties nušalimo atvejis. Mūsų pristatomas atvejis – gilus III laipsnio krūties nu­šalimas, kuriam gydyti prireikė ir operacijos. Šiame pranešime aprašoma dvidešimt devynerių metų žindančios pacientės patirtis, kai dėl mastito moteris dėjo ant krūties ledo paketą ir užmigo. Po dviejų dienų ji kreipėsi į Lietuvos sveikatos mokslų universiteto ligoninę Kauno klinikas dėl krūties skausmo, paraudimo, patinimo ir karščiavimo. Apžiūrint kairioji krūtis buvo kieta, edemiška, paraudusi visame plote, žaizdos apatiniuose kvadrantuose su negyvybingų audinių ploteliais. Ankstyvuoju gydymo laikotarpiu buvo pasirinkta konservatyvi taktika, žaizdos tvarstytos antiseptiku suvilgytais tvarsčiais, išryškėjus negy­vybingų audinių ribai, tie audiniai buvo šalinami etapais. Žaizdos gijimą sunkino pieno skyrimasis į žaizdos dugną. Vėliau ope­racijos metu pieno latakai buvo persiūti, o susidaręs defektas padengtas atliekant odos ir poodžio plastiką. Eigą po operacijos komplikavo P. aeruginosa žaizdos infekcija, tačiau paskyrus tikslinę antibiotikų terapiją žaizdos infekcijos požymiai atslūgo, žaizda sugijo. Šiame klinikinio atvejo pranešime aprašomas sėkmingas gilaus krūties nušalimo su minkštųjų audinių defektu gydymas pasitelkiant konservatyvias priemones bei plastinės ir rekonstrukcinės chirurgijos metodus.
Patrones de acceso al Portal Web Guías de Práctica Clínica en Colombia
Authors: Suárez-Obando Fernando , Gómez-Restrepo Carlos
Published: March 2017

Introducción. Las guías de práctica clínica colombianas para la atención sanitaria se difunden en el Portal Web del Ministerio de Salud y Protección Social.Objetivo. Analizar el tráfico del portal de guías clínicas a través de métricas de consulta web.Materiales y métodos. Se realizó análisis del tráfico del portal en un periodo de 20 meses utilizando Google Analytics y Megalytic.Resultados. Se registraron 190 115 ingresos, 125 475 (≈66%) fueron visitantes de primera vez y 63 118 (≈33%) usuarios repetidos. 126 994 usuarios visitaron 608 745 páginas, con un promedio de 3.2 páginas por sesión, tiempo de consulta de 3.45 minutos por visita y tasa de rebote promedio del 46.74%. 40% de los usuarios interactuó al menos con tres páginas y también 40% abandonaron el portal sin interactuar con una segunda página. Las sesiones se originaron desde Colombia, México, Perú y España, en el primero se presentaron 169 666 y Bogotá, D.C. registró el mayor número de visitas (32%), seguido de Medellín (12.3%), Cali (8.3%), Barranquilla (4.1%) y Bucaramanga (3.3%); estas cinco ciudades acumulan el 60% del tráfico. Las guías más visitadas fueron las de manejo de embarazo e infección de tracto urogenital.Conclusiones. El portal presenta tráfico aceptable en los primeros 20 meses de funcionamiento. Se debe mantener un portal innovador que mejore la difusión de las guías.
LA RED SOCIOTÉCNICA ORIGINADA EN COSTA RICA, QUE PERMITIÓ EL DESARROLLO DEL PRIMER SUERO ANTIOFÍDICO POLIVALENTE PARA ÁFRICA SUBSAHARIANA
Authors: Calderón Saravia Ana Lucía
Published: March 2017

Este artículo trata un estudio de caso de una comunidad tecnocientífica, el InstitutoClodomiro Picado Twight y el desarrollo del primer suero antiofídico polivalente para ÁfricaSubsahariana. Pretende aportar algunos elementos teórico conceptuales generados desdelas investigaciones sociales de la ciencia y la tecnología o la denominada corriente Ciencia,Tecnología y Sociedad (cts), para el estudio de comunidades científicas y tecnológicas, suproducción de conocimiento y artefactos, así como algunos aportes desde la Antropología
TENDENCIAS, FRACTURAS Y QUIEBRES EN LA DISCURSIVIDAD POLÍTICO-ELECTORAL COSTARRICENSE. ANÁLISIS POLÍTICODISCURSIVO DURANTE EL PROCESO DE LA CAMPAÑA PRESIDENCIAL 2013-2014
Authors: Carballo Chaves* Pablo
Published: March 2017

Las recientes elecciones presidenciales en Costa Rica (2013-2014) dejaron ver una serie decircunstancias que van desde tendencias históricas, pasando por fracturas hasta quiebressocio-simbólicos que han recompuesto el escenario de los mensajes políticos. Este artículorealiza una introducción al estudio de dicho proceso desde tres dimensiones interrelacionadas:los partidos políticos, los candidatos y los elementos de la cultura política costarricense.Se cierra con una conclusión sobre la tendencia a la personalización como factor básicoen la era de los medios de comunicación y la individualización, con una fractura en la disputapor los símbolos políticos legítimos como la centralidad y finalmente, con un quiebreen el sentido de una incorporación reciente en la terminología de uso en los discursos ymensajes electorales que dan un matiz distinto y con diferentes horizontes para el estudiode la comunicación política costarricense.
El capital social y el incremento en la oferta de cursos de idiomas en la Universidad de Costa Rica: experiencias del curso Catalán Básico I
Authors: Blanco Obando Edgar Eduardo
Published: March 2017

Este artículo presenta la apertura del curso Catalán Básico I en la Escuela de Lenguas Modernas (ELM) de la Universidad de Costa Rica (UCR), como resultado del capital social generado dentro de la misma Universidad. A su vez, propone crear espacios y fomentar la participación solidaria de los universitarios para obtener mayores proyectos de beneficio público y bien común. This article presents the course Catalan Basic I of the School of Modern Languages at the University of Costa Rica, as a result of university’s social capital. At the same time, it aims to promote academic’s voluntary work to increase projects of common good.
PENGARUH PUASA TERHADAP PERTUMBUHAN DAN POLA KEMATIAN PADA BENIH IKAN KERAPU TIKUS, cromiteptes attivetis
Authors: Setiawati Ketut Maha , Hutapea John Haryanto , Widianingsih Widianingsih
Published: March 2017

Penelitian ini dilakukan untuk mengetahui pengaruh lamanya puasa terhadap pertumbuhan dan sintasan benih kerapu tikus dan pola kematian benih yang diakibatkan oleh pemuasaan (tanpa diberi pakan) secara terus-menerus.
RESPON HISTOLOGIS TUBUH KODOK (Rana catesbeiana Shaw) TERHADAP INFEKSI BAKTERI PATOGEN DAN POTENSI Saccharomyces cerevisiae SEBAGAI IMUNOSTIMULAN
Authors: Taukhid Taukhid , Taufik Pipik , Mundriyanto Honorius
Published: March 2017

Penelitian ini bertujuan untuk mengetahui respon histologis tubuh kodok (Rana catesbeiana Shaw) terhadap infeksi bakteri patogen serta potensi ekstrak Saccharomyces cerevisiae terhadap peningkatan respon imunitas tubuh kodok yang diberikan melalui pakan didahului dengan pnming.
AKUMULASI LOGAM BERAT Pb DALAM TUBUH UDANG WINDU (Penaeus monodon) PADA KONDISI SALINITAS BERBEDA
Authors: Pong-Masak Petrus Rani , Rachmansyah Rachmansyah
Published: March 2017

Penelitian ini bertujuan untuk mengetahui pengaruh tingkat salinitas media pemeliharaan terhadap konsentrasi akumulasi logam Pb dalam tubuh udang windu (Penaeus mrsnodon).
POPULASI DAN TINGKAT PEMANFAATAN BULU BABI (Echinoidea) DI PADANG LAMUN PULAU OSI, SERAM BARAT, MALUKU TENGAH
Authors: Syam Amran Ronny , Edrus Lsa Nagib , Andamari Retno
Published: March 2017

Penelitian populasi bulu babi dan pemanfaatannya di perairan padang lamun Pulau Osi telah dilakukan pada bulan Juni 1995 sampai dengan Februari 1996. Metode transek kuadrat digunakan untuk menduga kepadatan populasi bulu babi tersebut. lndeks agregasi digunakan sebagai indikator dari sifat distribusi spasial populasi itu.
KAJIAN SIFAT FISIOLOGI KERAPU LUMPUR (Epinephelus tauvina) SEBAGAI DASAR DALAM PENGEMBANGAN TEKNIK TRANSPORTASI HIDUP
Authors: Wibowo Singgih , Suryaningrum Th. Dwi , Utomo Bagus Sediadi Bandol
Published: March 2017

Penelitian mengenai sifat fisiologi ikan kerapu yang meliputi aktivitas, kecepatan respirasi dan metabolisme pada berbagai suhu rendah sebagai dasar pengembangan teknologi transportasi ikan kerapu hidup telah dilakukan.
PENELITIAN FORTIFIKASI IKAN PADA PENGOLAHAN PATOLO
Authors: Subaryono Subaryono , N. Indriati N. Indriati , Irianto Hari Eko
Published: March 2017

Penelitian pengolahan patolo (keripik singkong fermentasi khas Gunung Kidul) dengan penambahan ikan telah dilakukan. Jenis ikan yang ditambahkan yaitu udang putih(Penaeus merguiensis), kerang darah (Anadara granosa) dan ikan beloso (Sauida tumbit).
AKTIVITAS PROTEOLITIK EKSTRAK PILORIK KAEKA IKAN TONGKOL (Aurius thazard)
Authors: Murniyat Murniyati , Heruwati Endang Sri
Published: March 2017

Organ isi perut ikan khususnya pilorik kaeka, telah diketahui berpotensi sebagai sumber enzim proteolitik, yang diketahui banyak manfaatnya baik dalam industri pangan maupun non pangan
ANÁLISE DO PROCESSO DE INTERNACIONALIZAÇÃO EM MPES: ESTUDO DE CASO DA CACHAÇA EXTREMA
Authors: Rocha Jéssica Barros da Silva , Camelo Gerda Lucia Pinheiro
Published: March 2017

O comércio exterior existe desde o início das civilizações e se justifica devido às diferenças existentes entre os países, o que possibilitou diversos acordos ao longo da história e a busca por vatagens competitivas. No mundo globalizado atual, tais diferenças e arranjos ganharam novas formas e tendências, empresas de todos os portes lidam com o comércio exterior e sua crescente relevância para sua sobrevivência em diferentes escalas, inclusive em seu mercado doméstico com a concorrência internacional. Este trabalho objetiva mostrar a importância do conhecimento acerca do processo de internacionalização para a inserção de uma empresa no mercado externo, vislumbrando a realidade das micro e pequenas empresas dentro deste cenário e como tal conhecimento é ainda mais crucial para estas. Para tal, a metodologia utilizada é composta por uma pesquisa intervencionista, que proporcionou maior diálogo com o objeto de estudo, e um estudo de caso de um produto, a Cachaça Extrema, que uma empresa potiguar de pequeno porte está internacionalizando.

PALAVRAS-CHAVE: Comércio exterior. Internacionalização. Micro e pequenas empresas.
A COMPETITIVIDADE DA INDÚSTRIA POTIGUAR FRENTE À CRISE ECONOMICA DE 2008: UM ESTUDO DE CASO MÚLTIPLO
Authors: Silva Raissa Costa da , Costa Lúcia De Fátima Lúcio Gomes da
Published: March 2017

O presente trabalho visa compreender em que aspectos a crise econômica que eclodiu no mundo em 2008 influenciou a competitividade das indústrias do Rio Grande do Norte. Para isso, foi feito um estudo de caso múltiplo, com a técnica de análise de conteúdo no qual foram entrevistadas quatro empresas industriais instaladas no estado, que foram escolhidas de acordo com a importância econômica para o estado; sendo feita toda a análise da convergência teórica com os fatos que ocorreram em cada empresa estudada, nos períodos pré-crise e pós-crise em relação às estratégias macro e microeconômicas da empresa e aos principais impactos diretos da crise sobre elas. Como resultado, foi obtido que a crise influenciou a competitividade das indústrias em alguns termos, como na área da tributação e na obtenção de insumos; mas em outros pontos, como a logística, a empresa depende de medidas governamentais para que as indústrias do estado sejam mais competitivas.
Participação Política no Brasil e na Suécia: o papel dos estereótipos e do contágio
Authors: Carneiro Thiago Lopes , Torres Cláudio Vaz , Ekman Joakim
Published: March 2017

RESUMO Este estudo investigou a influência de estereótipos sobre parlamentares e contágio comportamental na participação política, comparando dois países: Brasil e Suécia. Considerou-se que estereótipos podem ser úteis para diferenciar grupos de parlamentares e predizer seus comportamentos. O Contágio Comportamental foi investigado como um possível catalisador da ação política. Os questionários online foram respondidos por 984 brasileiros (37,4% mulheres) e 879 suecos (46,5% mulheres). Empregou-se a Modelagem por Equações Estruturais para aferir as relações entre as variáveis. O Contágio Comportamental foi central na predição do engajamento. Os estereótipos predisseram participação quando desafiavam o senso comum: brasileiros não costumam diferenciar parlamentares, mas aqueles que conseguiam eram mais engajados; na Suécia, o fator “corrupção” predisse a participação não-institucional.
Morphological, biochemical, and molecular characterization of Meloidogyne spp. populations from Brazilian soybean production regions
Authors: Oliveira Camilla Martins de , Souza Junior Ismail Teodoro , Araújo Filho Jerônimo Vieira de , Freitas Marcos Augusto de , Rocha Mara Rúbia da , Petrofeza Silvana
Published: March 2017

ABSTRACT: Soybean is a commodity of great economic importance worldwide, particularly in Brazil, world’s second largest producer. Nematodes, especially those of the Meloidogyne genus, severely limit productivity. Identification of nematode species is important for effective soybean management. Here, 26 populations of root-knot nematode (Meloidogyne spp.) from 15 municipalities in the states of Bahia, Mato Grosso, Goias, and Minas Gerais were characterized based on the morphology of the female perineal region, esterase profile, and identification based on amplification of specific regions of the population genome. Among the Meloidogyne spp. populations obtained, M. incognita and M. javanica, were identified. No mixed populations were present in the samples. Diagnosis based on molecular analysis was shown to be reliable and the fastest for characterization of nematode populations compared to other methods analyzed.