bluuebunny/crossref_metadata_2025 huggingface dataset:
Rethinking Neandertals
Authors: Nowell April
Published: July 2023

In this article, I first provide an overview of the Neandertals by recounting their initial discovery and subsequent interpretation by scientists and by discussing our current understanding of the temporal and geographic span of these hominins and their taxonomic affiliation. I then explore what progress we have made in our understanding of Neandertal lifeways and capabilities over the past decade in light of new technologies and changing perspectives. In the process, I consider whether these advances in knowledge qualify as so-called Black Swans, a term used in economics to describe events that are rare and unpredictable and have wide-ranging consequences, in this case for the field of paleoanthropology. Building on this discussion, I look at ongoing debates and focus on Neandertal extinction as a case study. By way of discussion and conclusion, I take a detailed look at why Neandertals continue to engender great interest, and indeed emotion, among scientists and the general public alike.
Medium Response and Jet–Hadron Correlations in Relativistic Heavy-Ion Collisions
Authors: Cao Shanshan , Qin Guang-You
Published: July 2023

The study of high-energy heavy-ion collisions at the Relativistic Heavy Ion Collider and the Large Hadron Collider has evolved from a qualitative understanding to the precise extraction of the properties of the quantum chromodynamics medium at extremely high temperatures. Jet quenching has offered unique insights into the transport properties of the quark–gluon plasma (QGP) created in these energetic collisions. Apart from medium modification of jets, jet-induced medium excitation constitutes another crucial aspect of jet–QGP interaction and is indispensable in understanding the soft components of jets. We review recent theoretical and phenomenological developments regarding medium response to jet energy loss, including an overview of both weakly and strongly coupled approaches for describing the thermalization and propagation of energy deposition from jets, effects of medium response on jet observables, and exploration of its unique signatures in jet–hadron correlations. Jet-induced medium excitation is shown to be an essential component in probing the in-medium dynamics of jets and a critical step toward precise extraction of the QGP properties.
Synergistic Effects of Nanoporous Structure of Glassy Carbon Electrode and Electropolymerization of Catechol Film on the Electrocatalytic Oxidation of Hydrazine
Authors: Khoshfetrat Seyyed Mehdi , Yari Mahdi , Dorraji Parisa Seyed , Jalali Fahimeh
Published: July 2023

Non-precious metal-free electrocatalysts with high sensitivity, selectivity, and long-term stability, as well as extraordinary activity towards hydrazine (HZ) oxidation are challenging and desirable for various fields. Herein, a binder-free catechol (CC)-immobilized nanoporous GCE was conducted to electrooxidation of HZ at extremely low overpotential with fast response time (∼3 s). Owing to synergistic-assisted signal amplification between the CC and nanoporous GCE, and nanoporous GCE’s high-effective-surface-area for incorporation of CC within the pores of nanoporous structure, high sensitive detection of HZ along with long-term stability has been introduced. Moreover, binder-free modification resulting in increased accessibility of the HZ to active surface sites of the electrode, which in turn increased the electrode performance. The affecting factors on the anodic signal for determination of hydrazine were optimized by response surface methodology. An optimized limit of detection of 0.14 μM was obtained at a signal to noise ratio of 3 and a fast response time (∼3 s). Additionally, CC/GCE-red revealed higher sensitivity with two linear ranges from 0.3 to 8.6 and 8.6 to 120 μM. The suggested sensor’s promising qualities include its ease of manufacture and good stability of the modified electrode.
Dysregulation of TMEM16A impairs oviductal transport of embryos
Authors: Ning Nannan , Luo Dan , Xia Wei , Mou Guangjing , Zhao Jiangli , Zhang Jian , Li Cheng , Wang Hongchun , Li Jingxin
Published: July 2023

The ion channel protein TMEM16A is expressed in the epithelium and smooth muscle of the human fallopian tube and is upregulated in patients with tubal pregnancy. TMEM16A is involved in regulating the smooth muscle contraction and the cilia beating. Dysregulated TMEM16A may result in embryo retention in the oviduct and delayed early embryo development. Our study reveals a new regulatory point for embryo transport and development.
Nestin identifies a subpopulation of rat ventricular fibroblasts and participates in cell migration
Authors: Hertig Vanessa , Villeneuve Louis , Calderone Angelino
Published: July 2023

Tcf21/WT1(+)-fibroblast progenitor cells of the embryonic mouse heart predominantly express the intermediate filament protein nestin. A subpopulation of Tcf21/WT1(+)-neonatal rat ventricular fibroblasts express nestin and are refractory to selective stimuli influencing cell cycle reentry. Scar-derived myofibroblasts plated in Matrigel elicit the formation of lumen-like structures characterized by the appearance of nestin(+)-membrane projections. Lentiviral shRNA-mediated nestin depletion in a subpopulation of neonatal rat ventricular fibroblasts suppressed the migratory response following the in vitro scratch assay.
The SLC38A5/SNAT5 amino acid transporter: from pathophysiology to pro-cancer roles in the tumor microenvironment
Authors: Taurino Giuseppe , Chiu Martina , Bianchi Massimiliano G. , Griffini Erika , Bussolati Ovidio
Published: July 2023

The transporter SLC38A5/SNAT5 provides net influx or efflux of glutamine, asparagine, and serine. These amino acids are of particular metabolic relevance in several conditions. Changes in transporter expression or activity have been described in selected types of human cancers, where SNAT5 can mediate amino acid exchanges between tumor and stromal cells, thus providing a potential therapeutic target. This is the first review that recapitulates the characteristics and roles of the transporter in physiology and pathology.
Senescence of alveolar epithelial progenitor cells: a critical driver of lung fibrosis
Authors: Parimon Tanyalak , Chen Peter , Stripp Barry R. , Liang Jiurong , Jiang Dianhua , Noble Paul W. , Parks William C. , Yao Changfu
Published: July 2023

With an aging population, lung fibrotic diseases are becoming a global health burden. Dysfunctional repair of the alveolar epithelium is a key causative process that initiates lung fibrosis. Normal alveolar regeneration relies on functional progenitor cells; however, the senescence of these cells, which increases with age, hinders their ability to contribute to repair. Here, we discuss studies on the control and consequence of progenitor cell senescence in fibrosis and opportunities for research.
G-protein tonic inhibition of calcium channels in pancreatic β-cells
Authors: Quiroz-Acosta Tayde , Bermeo Karina , Arenas Isabel , Garcia David E.
Published: July 2023

Our results support the hypothesis that GPCRs tonically inhibit HVA Ca2+ channels in pancreatic β-cells. A voltage-clamp protocol with a strong depolarizing prepulse was used to unmask voltage-dependent inhibition of Ca2+ channels. The novelty of these results strengthens the critical role of Gβγ’s in Ca2+ channel regulation, highlighting kinetic slowing and increased facilitation ratio. Together, HVA Ca2+ channels of pancreatic β-cells are constitutively inhibited by GPCRs underlying fine-tuning modulation of insulin secretion.
Dose-dependent progression of multiple low-dose streptozotocin-induced diabetes in mice
Authors: Bauer Brandon M. , Bhattacharya Supriyo , Bloom-Saldana Elizabeth , Irimia-Dominguez Jose M. , Fueger Patrick T.
Published: July 2023

This study investigated the effects of different multiple low doses of streptozotocin (STZ), namely 35 and 55 mg/kg, on the onset and progression of diabetes in mice. Both doses are commonly used in research, and although both induced a loss of beta cell mass, they had distinct effects on whole glucose tolerance, beta cell function, and gene transcription. Mice treated with 55 mg/kg became rapidly glucose intolerant, whereas those treated with 35 mg/kg had a slower onset and remained glucose tolerant for up to a week before becoming equally glucose intolerant as the 55 mg/kg group. Beta cell mass loss was similar between the two groups, but the 35 mg/kg-treated mice had improved glucose-stimulated insulin secretion in gold-standard hyperglycemic clamp studies. Transcriptomic analysis revealed that the 55 mg/kg dose caused disruptions in nearly five times as many genes as the 35 mg/kg dose in isolated pancreatic islets. Pathways that were downregulated in both doses were more downregulated in the 55 mg/kg-treated mice, whereas pathways that were upregulated in both doses were more upregulated in the 35 mg/kg-treated mice. Moreover, we observed a differential downregulation in the 55 mg/kg-treated islets of beta cell characteristic pathways, such as exocytosis or hormone secretion. On the other hand, apoptosis was differentially upregulated in 35 mg/kg-treated islets, suggesting different transcriptional mechanisms in the onset of STZ-induced damage in the islets. This study demonstrates that the two STZ doses induce distinctly mechanistic progressions for the loss of functional beta cell mass.
Gene coexpression network analysis reveals perirenal adipose tissue as an important target of prenatal malnutrition in sheep
Authors: Ahmad Sharmila , Drag Markus Hodal , Mohamad Salleh Suraya , Cai Zexi , Nielsen Mette Olaf
Published: July 2023

We have previously demonstrated that pre- and early postnatal malnutrition in sheep induced depot- and sex-specific changes in adipose morphological features, metabolic outcomes, and transcriptome in adulthood, with perirenal (PER) as the major target followed by subcutaneous (SUB) adipose tissue. We aimed to identify coexpressed and hub genes in SUB and PER to identify the underlying molecular mechanisms contributing to the early nutritional programming of adipose-related phenotypic outcomes. Transcriptomes of SUB and PER of male and female adult sheep with different pre- and early postnatal nutrition histories were used to construct networks of coexpressed genes likely to be functionally associated with pre- and early postnatal nutrition histories and phenotypic traits using weighted gene coexpression network analysis. The modules from PER showed enrichment of cell cycle regulation, gene expression, transmembrane transport, and metabolic processes associated with both sexes’ prenatal nutrition. In SUB (only males), a module of enriched adenosine diphosphate metabolism and development correlated with prenatal nutrition. Sex-specific module enrichments were found in PER, such as chromatin modification in the male network but histone modification and mitochondria- and oxidative phosphorylation-related functions in the female network. These sex-specific modules correlated with prenatal nutrition and adipocyte size distribution patterns. Our results point to PER as a primary target of prenatal malnutrition compared to SUB, which played only a minor role. The prenatal programming of gene expression and cell cycle, potentially through epigenetic modifications, might be underlying mechanisms responsible for observed changes in PER expandability and adipocyte-size distribution patterns in adulthood in both sexes.
Delayed Urethral Obstruction after Migration of a Ballistic Pellet in an Alpine Wether
Authors: Smith Joe S. , Hespel Adrien-Maxence , Garcia Jessica D. , Lipe Krista L. , Kleine Stephanie A. , Mulon Pierre-Yves
Published: July 2023

A one-year-old alpine wether was presented for emergency evaluation of stranguria. Diagnostics identified a moderately distended bladder and mild dehydration. Preliminary lateral radiographs identified two metallic structures consistent with projectile pellets in the pelvic and perineal regions and no evidence of radiopaque uroliths. A tube cystostomy was performed, and a contrast urethrogram revealed one of the pellets in the perineal region was in proximity to the urethral obstruction. Subsequent radiography and ultrasound identified the pellet as being within the lumen of the urethra. Examination of the trichotomized skin revealed two scars, including a scar over the paralumbar fossa in the region of the urinary bladder suggestive of a projectile injury. The pellet was removed by a perineal urethrotomy. The patient was able to spontaneously urinate after urethrotomy, passed a tube cystostomy challenge two weeks after surgery, and was discharged. No complications were reported. While uncommon in the veterinary and comparative medical literature, clinicians should consider the potential for projectile pellets to migrate into the urinary tract after initial injury.
An Efficient Load Prediction‐Driven Scheduling Strategy Model in Container Cloud
Authors: Wang Lu , Guo Shuaidong , Zhang Pengli , Yue Haodong , Li Yaxiao , Wang Chenyi , Cao Zhuang , Cui Di
Published: July 2023

The rise of containerization has led to the development of container cloud technology, which offers container deployment and management services. However, scheduling a large number of containers efficiently remains a significant challenge for container cloud service platforms. Traditional load prediction methods and scheduling algorithms do not fully consider interdependencies between containers or fine‐grained resource scheduling, leading to poor resource utilization and scheduling efficiency. To address these challenges, this paper proposes a new load prediction model CNN‐BiGRU‐Attention and a container scheduling strategy based on load prediction. The prediction model CNN and BiGRU focus on the local features of load data and long sequence dependencies, respectively, as well as introduce the attention mechanism to make the model more easily capture the features of long distance dependencies in the sequence. A container scheduling strategy based on load prediction is also designed, which first uses the load prediction model to predict the load state and then generates a scheduling strategy based on the load prediction value to determine the change of the number of container replicas in a fine‐grained manner based on the load prediction value in the next time window, while the established domain‐based container selection method is employed to facilitate the coarse‐grained online migration of containers. Experiments conducted using public datasets and open‐source simulation platforms demonstrate that the proposed approach achieves a 37.4% improvement in container load prediction accuracy and a 21.7% improvement in container scheduling efficiency compared to traditional methods. These results highlight the effectiveness of the proposed approach in addressing the challenges faced by container cloud service platforms.
Effects of Rotten Vegetable-Based Low-Cost Media on the Growth and Morphology of an Astaxanthin-Producing Green Alga, Monoraphidium Littorale
Authors: Ritu Jinnath Rehana , Khan Saleha , Hossain Md. Sakhawat , Haque Md. Mahfuzul
Published: July 2023

Growing microalgae on vegetable waste (rotten potatoes) media is not only a plausible technique to replace expensive commercial media but also a way of reducing feed costs for rearing zooplankton and fish larvae in aquaculture. As a way of starting an inevitable step up, Monoraphidium littorale was grown in 25% (T1), 50% (T2), and 75% (T3) of digested rotten potato supernatant (DRPS) and at the same time in Bold Basal Medium (BBM) as a control (T4) for 16 days. The highest cell density of M. littorale was recorded in T1, followed by T4, T2, and T3. A similar increasing trend of chlorophyll-a and optical density was also observed during the experimental period. The study also ascertained T1 as a potential growth medium where M. littorale showed superior biomass production which was significantly higher (

P
<
0.05

) than other treatments. It was also worth mentioning that the highest (

P
<
0.05

) protein content was determined in T1 at the end of the cultivation time. Similarly, a highly significant difference (

P
<
0.05

) was also found in the lipid content of the microalgae grown in T1 and T4. Furthermore, the morphology of the cells of M. littorale was found to be strongly affected by the DRPS concentrations and BBM. Thus, the lower concentration of the DRPS served as the best medium for enhancing the growth and biomass of M. littorale. Consequently, to expedite the sustainable progression of microalgal production, cost-effective culture techniques by using different wastes may be adopted.
Supplementary table 3 from Spectrum of Response to Platinum and PARP Inhibitors in Germline BRCA–Associated Pancreatic Cancer in the Clinical and Preclinical Setting
Authors: Stossel Chani , Raitses-Gurevich Maria , Atias Dikla , Beller Tamar , Glick Gorman Yulia , Halperin Sharon , Peer Eyal , Denroche Robert E. , Zhang Amy , Notta Faiyaz , Wilson Julie M. , O'Kane Grainne M. , Haimov Talmoud Elina , Amison Nora , Schvimer Michael , Salpeter Seth J. , Bar Vered , Zundelevich Adi , Tirosh Itay , Tal Rotem , Dinstag Gal , Kinar Yaron , Eliezer Yonatan , Ben-David Uri , Gavert Nancy S. , Straussman Ravid , Gallinger Steven J. , Berger Raanan , Golan Talia
Published: July 2023

20 genes in DDR resistance
Supplemental Figure 6 from Siglec-15 Promotes Evasion of Adaptive Immunity in B-cell Acute Lymphoblastic Leukemia
Authors: Pillsbury Claire E. , Dougan Jodi , Rabe Jennifer L. , Fonseca Jairo A. , Zhou Chengjing , Evans Alyssa N. , Abukharma Hasan , Ichoku Ona , Gonzalez-Flamenco Gloria , Park Sunita I. , Aljudi Ahmed , DeRyckere Deborah , Castellino Sharon M. , Rafiq Sarwish , Langermann Solomon , Liu Linda N. , Henry Curtis J. , Porter Christopher C.
Published: July 2023

Sig15 ablation in murine B-ALL promotes an adaptive immune response against leukemia.
Supplemental Figure 4 from Siglec-15 Promotes Evasion of Adaptive Immunity in B-cell Acute Lymphoblastic Leukemia
Authors: Pillsbury Claire E. , Dougan Jodi , Rabe Jennifer L. , Fonseca Jairo A. , Zhou Chengjing , Evans Alyssa N. , Abukharma Hasan , Ichoku Ona , Gonzalez-Flamenco Gloria , Park Sunita I. , Aljudi Ahmed , DeRyckere Deborah , Castellino Sharon M. , Rafiq Sarwish , Langermann Solomon , Liu Linda N. , Henry Curtis J. , Porter Christopher C.
Published: July 2023

Sig15 is required for immune evasion by murine B-ALL.
Supplemental Figure 3 from Siglec-15 Promotes Evasion of Adaptive Immunity in B-cell Acute Lymphoblastic Leukemia
Authors: Pillsbury Claire E. , Dougan Jodi , Rabe Jennifer L. , Fonseca Jairo A. , Zhou Chengjing , Evans Alyssa N. , Abukharma Hasan , Ichoku Ona , Gonzalez-Flamenco Gloria , Park Sunita I. , Aljudi Ahmed , DeRyckere Deborah , Castellino Sharon M. , Rafiq Sarwish , Langermann Solomon , Liu Linda N. , Henry Curtis J. , Porter Christopher C.
Published: July 2023

Sig15 localizes to the Golgi apparatus and TGN in B-ALL.
FIGURE 5 from Siglec-15 Promotes Evasion of Adaptive Immunity in B-cell Acute Lymphoblastic Leukemia
Authors: Pillsbury Claire E. , Dougan Jodi , Rabe Jennifer L. , Fonseca Jairo A. , Zhou Chengjing , Evans Alyssa N. , Abukharma Hasan , Ichoku Ona , Gonzalez-Flamenco Gloria , Park Sunita I. , Aljudi Ahmed , DeRyckere Deborah , Castellino Sharon M. , Rafiq Sarwish , Langermann Solomon , Liu Linda N. , Henry Curtis J. , Porter Christopher C.
Published: July 2023

Sig15 is required for adaptive immune escape in murine B-ALL. qRT-PCR shows genetic knockdown of Siglec15 expression in a murine model of B-ALL by shRNA (shSig15; A) or knocked out using CRISPR/Cas9 (Sig15 KO; B). Data are normalized to the expression levels of a non-silencing vector control (shNS) or Cas9-expressing cells without gRNA (Cas9). C and D, Unirradiated WT or Rag1−/− C57BL6 mice were injected via tail vein with 5 × 105 control or Sig15-deficient leukemia cells. Luciferase signal over time represents disease burden as measured via IVIS imaging over 14 days. Kaplan–Meier curve shows prolonged survival of WT recipients of Sig15-deficient leukemia. E,P < 0.0001; shNS WT versus shSig15 WT, Mantel–Cox log-rank test; n = 10 per group from two independent experiments. F,P < 0.0001, Cas9-only WT versus Sig15 KO WT; n = 10 per group from two independent experiments.
FIGURE 4 from Siglec-15 Promotes Evasion of Adaptive Immunity in B-cell Acute Lymphoblastic Leukemia
Authors: Pillsbury Claire E. , Dougan Jodi , Rabe Jennifer L. , Fonseca Jairo A. , Zhou Chengjing , Evans Alyssa N. , Abukharma Hasan , Ichoku Ona , Gonzalez-Flamenco Gloria , Park Sunita I. , Aljudi Ahmed , DeRyckere Deborah , Castellino Sharon M. , Rafiq Sarwish , Langermann Solomon , Liu Linda N. , Henry Curtis J. , Porter Christopher C.
Published: July 2023

B-ALL cells release secreted/soluble Sig15 that circulates in plasma and inhibits activation of CAR-expressing Jurkat cells. A, sSIG15 detected via electrochemiluminescence (MSD) from the supernatant of REH cells treated for 24 hours with combinations of PMA (81 nmol/L), BOT64 (10 μmol/L), and CSA (1 μmol/L). Dotted line represents the assay limit of detection (10 pg/mL). PMA induces a calcineurin-dependent increase in the release of sSig15. B, sSIG15 detected via MSD from the plasma of pediatric patients with B-ALL, AML or T-ALL and healthy controls (sample size in parentheses). Pediatric patients with B-ALL showed higher plasma concentration of sSIG15 than healthy donors (****, P < 0.0001). C, Cytokines were measured in the plasma from children with leukemia using Luminex and compared with sSig15. Pearson correlation of a subset is depicted graphically using the nearest neighbor algorithm (*, P < 0.05). D and E, Jurkat cells expressing CD19-CAR constructs cultured alone (UT = untreated) or cocultured with CD19+ REH B-ALL cells (REH) at an effector:target ratio of 2:1 with or without 50 μg/mL protein control (Ctrl = human IgG) or recombinant Sig15 (rSig15) for 4 hours and stained for early activation markers CD69 and PD-1. D, Representative contour plots of flow cytometry data. E, Quantification of four independent experiments. Recombinant Sig15 inhibits early Jurkat activation when in coculture with B-ALL cells.
FIGURE 2 from Siglec-15 Promotes Evasion of Adaptive Immunity in B-cell Acute Lymphoblastic Leukemia
Authors: Pillsbury Claire E. , Dougan Jodi , Rabe Jennifer L. , Fonseca Jairo A. , Zhou Chengjing , Evans Alyssa N. , Abukharma Hasan , Ichoku Ona , Gonzalez-Flamenco Gloria , Park Sunita I. , Aljudi Ahmed , DeRyckere Deborah , Castellino Sharon M. , Rafiq Sarwish , Langermann Solomon , Liu Linda N. , Henry Curtis J. , Porter Christopher C.
Published: July 2023

Sig15 expression is regulated by NFκB activation in B-ALL. A, Western blot analysis shows upregulated SIG15 expression in REH cells stimulated for 24 hours with a PMA/ionomycin stimulation (81 nmol/L PMA and 1.3 μmol/L ionomycin) but not with 30 ng/mL of recombinant human MCSF and/or IL10. Densitometric analysis of SIG15 is quantified above. SIG15 was probed using the Invitrogen polyclonal antibody. B, qRT-PCR from REH cells treated with PMA (81 nmol/L) with or without pan-PKC inhibitor Gö 6983 (5 μmol/L) for 24 hours, showing that PKC inhibition abrogates SIG15 induction by PMA. (****, P < 0.0001). C, qRT-PCR from REH cells stimulated with PMA (81 nmol/L) and/or treated with calcineurin inhibitor CSA (1 μmol/L) or IKK-2 inhibitor BOT64 (10 μmol/L) for 24 hours, demonstrating that NFκB inhibition but not calcineurin inhibition abrogates PMA-induced SIG15 transcription (****, P < 0.0001). D, Western blot analysis shows SIG15 expression in REH cells following 24 hours of stimulation with 81 nmol/L PMA alone or in combination with 10 μmol/L of the IKK-2 inhibitor BOT64. NFκB inhibition attenuates PMA-induced upregulation of SIG15. SIG15 was probed using the 1F7 mAb (NextCure). E,SIG15 expression from the B-ALL TARGET database correlated with protein kinase C β (PRKCB; r = 0.35; P < 0.0001, Pearson correlation), calcineurin catalytic subunit alpha (PPP3CA; r = 0.42; P < 0.0001), and IL1β (IL1B; r = 0.33; P < 0.0001, Pearson correlation). F, Western blot analysis shows increased SIG15 protein in REH cells stimulated for 24 hours with increasing doses of recombinant human CD40L. Densitometric analysis of SIG15 is quantified above. SIG15 was probed using the 1F7 mAb (NextCure).
FIGURE 1 from Siglec-15 Promotes Evasion of Adaptive Immunity in B-cell Acute Lymphoblastic Leukemia
Authors: Pillsbury Claire E. , Dougan Jodi , Rabe Jennifer L. , Fonseca Jairo A. , Zhou Chengjing , Evans Alyssa N. , Abukharma Hasan , Ichoku Ona , Gonzalez-Flamenco Gloria , Park Sunita I. , Aljudi Ahmed , DeRyckere Deborah , Castellino Sharon M. , Rafiq Sarwish , Langermann Solomon , Liu Linda N. , Henry Curtis J. , Porter Christopher C.
Published: July 2023

Sig15 is highly expressed in B-ALL and additional hematologic malignancies. A, Relative SIG15 expression. B-ALL (N = 147; P = 3.6 × 10−19) and AML (N = 542; P = 2.0 × 10−9) samples showed higher SIG15 than normal PBMC samples (N = 74). Data adapted from Haferlach and colleagues through Oncomine.org (53). B, Relative SIG15 expression across a panel of common childhood cancers from the St. Jude PeCan database. Dotted red line indicates median expression for all tumors in the graph (*, P < 0.05; ****, P < 0.0001, ANOVA). Western blot analysis shows higher SIG15 expression across a panel of B-ALL (C), AML (D), and DLBCL human cell lines compared with normal healthy PBMCs (NML). No B-ALL or DLBCL cell lines show detectable levels of the Sig15 binding partner DAP12. SIG15 was probed using the Invitrogen polyclonal antibody. E, Representative flow cytometry of primary childhood B-ALL. Three of seven BMA samples had B-ALL cells which stained positive/dim-positive for Sig15 and one of eight PBL samples had B-ALL cells which stained positive. Zero of two hematogones from non-leukemia donor BMA samples and zero of six B cells from PBL samples from non-leukemia donors were positive for Sig15. Dotted lines represent the mean fluorescence intensity from normal hematogones and B cells. SIG15 was probed using the NP159 mAb (NextCure) conjugated to Alexa Fluor 647.
Supplementary Table S6 from Cancer Epidemiology in the Northeastern United States (2013-2017)
Authors: Rees Judy R , Weiss Julie E , Gunn Christine M , Carlos Heather A , Dragnev Nathalie C , Supattapone Emma Y , Tosteson Anna N.A. , Kraft Sally A , Vahdat Linda T , Peacock Janet L
Published: July 2023

Northern New England state and population characteristics
Supplementary Table S4 from Cancer Epidemiology in the Northeastern United States (2013-2017)
Authors: Rees Judy R , Weiss Julie E , Gunn Christine M , Carlos Heather A , Dragnev Nathalie C , Supattapone Emma Y , Tosteson Anna N.A. , Kraft Sally A , Vahdat Linda T , Peacock Janet L
Published: July 2023

Age-standardized Incidence of Cancer Maine, New Hampshire, Vermont, US Census Regions with the United States, All Races and by Race/Ethnicity, 2013-2017
Supplementary Table S3 from Cancer Epidemiology in the Northeastern United States (2013-2017)
Authors: Rees Judy R , Weiss Julie E , Gunn Christine M , Carlos Heather A , Dragnev Nathalie C , Supattapone Emma Y , Tosteson Anna N.A. , Kraft Sally A , Vahdat Linda T , Peacock Janet L
Published: July 2023

Patterns of incidence and mortality in Northern New England compared to the United States, among non-Hispanic White adults
Supplementary Figure 1 from Cancer Epidemiology in the Northeastern United States (2013-2017)
Authors: Rees Judy R , Weiss Julie E , Gunn Christine M , Carlos Heather A , Dragnev Nathalie C , Supattapone Emma Y , Tosteson Anna N.A. , Kraft Sally A , Vahdat Linda T , Peacock Janet L
Published: July 2023

United States Regions used in the analyses; Age-adjusted Cancer Incidence in the United States, 2015-2019, all races combined; Age-adjusted Cancer Mortality in the United States, 2015-2019, all races combined
Supplementary Figure 5 from Phase II window study of olaparib alone or with cisplatin or durvalumab in operable Head and Neck Cancer
Authors: Moutafi Myrto , Koliou Georgia-Angeliki , Papaxoinis George , Economopoulou Panagiota , Kotsantis Ioannis , Gkotzamanidou Maria , Anastasiou Maria , Pectasides Dimitrios , Kyrodimos Efthymios , Delides Alexander , Giotakis Evangelos , Papadimitriou Nikolaos G. , Panayiotides Ioannis G. , Perisanidis Christos , Fernandez Aileen I. , Xirou Vasiliki , Poulios Christos , Gagari Eleni , Yaghoobi Vesal , Gavrielatou Niki , Shafi Saba , Aung Thazin Nwe , Kougioumtzopoulou Andromachi , Kouloulias Vassilis , Palialexis Konstantinos , Gkolfinopoulos Stavros , Strati Areti , Lianidou Evi , Fountzilas George , Rimm David L. , Foukas Periklis G. , Psyrri Amanda
Published: July 2023

Supplementary Figure 5. Map showing the distribution of mutations per gene per tumor. Light and dark purple, green and orange indicate pre- and post-treatment samples for patients treated with cisplatin and olaparib, olaparib and durvalumab and olaparib only, respectively. Blues correspond to samples before and after second biopsy/surgery for patients who did not receive treatment.
Supplementary Video 2A from Targeted single cell isolation of spontaneously escaping live melanoma cells for comparative transcriptomics
Authors: Tearle Jacqueline L. E. , Arjunan Satya N.V. , Tay Szun S. , Colakoglu Feyza , Cremasco James , Golo Matteo , Biro Maté
Published: July 2023

Supplementary Video 2A shows representative images of a vehicle-treated spheroid. Scale bar: 200 µm, number at top left: days post-implantation.
Supplementary Table 4 from Targeted single cell isolation of spontaneously escaping live melanoma cells for comparative transcriptomics
Authors: Tearle Jacqueline L. E. , Arjunan Satya N.V. , Tay Szun S. , Colakoglu Feyza , Cremasco James , Golo Matteo , Biro Maté
Published: July 2023

Supplementary Table 4 shows Differentially expressed genes for amoeboid vs. escaping analysis
Supplementary Table 2 from Targeted single cell isolation of spontaneously escaping live melanoma cells for comparative transcriptomics
Authors: Tearle Jacqueline L. E. , Arjunan Satya N.V. , Tay Szun S. , Colakoglu Feyza , Cremasco James , Golo Matteo , Biro Maté
Published: July 2023

Supplementary Table 2 shows Transcript counts for common housekeeping genes, with cells excluded from the study due to low housekeeping expression highlighted in yellow
Supplementary Figure 9 from Targeted single cell isolation of spontaneously escaping live melanoma cells for comparative transcriptomics
Authors: Tearle Jacqueline L. E. , Arjunan Satya N.V. , Tay Szun S. , Colakoglu Feyza , Cremasco James , Golo Matteo , Biro Maté
Published: July 2023

Supplementary Figure 9 shows Escaped cell analyses for naltrindole and sulfasalazine spheroid assays
Supplementary Figure 8 from Targeted single cell isolation of spontaneously escaping live melanoma cells for comparative transcriptomics
Authors: Tearle Jacqueline L. E. , Arjunan Satya N.V. , Tay Szun S. , Colakoglu Feyza , Cremasco James , Golo Matteo , Biro Maté
Published: July 2023

Supplementary Figure 8 shows a summary of GSEA pathway analyses using Hallmarks gene set showing top 25 enriched pathways for each comparison
Supplementary Figure 6 from Targeted single cell isolation of spontaneously escaping live melanoma cells for comparative transcriptomics
Authors: Tearle Jacqueline L. E. , Arjunan Satya N.V. , Tay Szun S. , Colakoglu Feyza , Cremasco James , Golo Matteo , Biro Maté
Published: July 2023

Supplementary Figure 6 shows an Amoeboid cell cohort gallery
Supplementary Figure 2 from Targeted single cell isolation of spontaneously escaping live melanoma cells for comparative transcriptomics
Authors: Tearle Jacqueline L. E. , Arjunan Satya N.V. , Tay Szun S. , Colakoglu Feyza , Cremasco James , Golo Matteo , Biro Maté
Published: July 2023

Supplementary Figure 2 shows clustering of 44 single cells and their gene expression heat map based on 230 differentially expressed genes
Supplementary Figure 1 from Targeted single cell isolation of spontaneously escaping live melanoma cells for comparative transcriptomics
Authors: Tearle Jacqueline L. E. , Arjunan Satya N.V. , Tay Szun S. , Colakoglu Feyza , Cremasco James , Golo Matteo , Biro Maté
Published: July 2023

Supplementary Figure 1 shows data generation for the photoconvertible spheroid assay
Data from Phase II window study of olaparib alone or with cisplatin or durvalumab in operable Head and Neck Cancer
Authors: Moutafi Myrto , Koliou Georgia-Angeliki , Papaxoinis George , Economopoulou Panagiota , Kotsantis Ioannis , Gkotzamanidou Maria , Anastasiou Maria , Pectasides Dimitrios , Kyrodimos Efthymios , Delides Alexander , Giotakis Evangelos , Papadimitriou Nikolaos G. , Panayiotides Ioannis G. , Perisanidis Christos , Fernandez Aileen I. , Xirou Vasiliki , Poulios Christos , Gagari Eleni , Yaghoobi Vesal , Gavrielatou Niki , Shafi Saba , Aung Thazin Nwe , Kougioumtzopoulou Andromachi , Kouloulias Vassilis , Palialexis Konstantinos , Gkolfinopoulos Stavros , Strati Areti , Lianidou Evi , Fountzilas George , Rimm David L. , Foukas Periklis G. , Psyrri Amanda
Published: July 2023

Abstract
Purpose:We conducted a phase II randomized non-comparative window of opportunity trial to evaluate the inhibition of cellular proliferation and the modulation of immune microenvironment after treatment with Olaparib alone or in combination with Cisplatin or Durvalumab in operable Head and Neck Squamous Cell Carcinoma (HNSCC) patients.

Patients and Methods:Forty-one HNSCC patients were randomized to Cisplatin plus Olaparib (Arm A), Olaparib alone (Arm B), no treatment (Arm C) or Durvalumab plus Olaparib (Arm D). The primary endpoint was to evaluate the percentage of patients in each arm that achieved a reduction of at least 25% in Ki67. Secondary endpoints included objective response rate (ORR), safety and pathologic complete response rate (pCR). Paired baseline and resection tumor biopsies and blood samples were evaluated for pre-specified biomarkers.

Results:A decrease in Ki67 of at least 25% was observed in 44.8% of treated patients, as measured by quantitative immunofluorescence (QIF). The ORR among treated patients was 12.1%. pCR was observed in 2 patients. Two serious adverse events occurred in 2 patients.
PD-L1 levels were significantly higher after treatment in arms (A) and (D). Expression of CD163 and Colony Stimulating Factor 1 Receptor (CSF1R) genes, markers of M2 macrophages, increased significantly posttreatment whereas the expression of CD80 a marker of M1 macrophages decreased.

Conclusions:Preoperative Olaparib with Cisplatin or alone or with Durvalumab was safe in the preoperative setting and led to decrease in Ki67 in 44.8% of treated patients. Olaparib-based treatment modulates the tumor microenvironment leading to PD-L1 upregulation and induction of pro-tumor features of macrophages.
Revision of the African Neoperla Needham, 1905 (Plecoptera: Perlidae: Perlinae) based on morphological and molecular data
Authors: ZWICK PETER , ZWICK ANDREAS
Published: July 2023

Adults of the African species of the genus Neoperla Needham, 1905 (Plecoptera: Perlidae: Perlinae) are revised, and 82 valid species are recognised. Of the original 29 named species, 14 valid ones are redescribed from types, nine lectotypes are designated, and nine new synonymies are proposed. Sixty-two new species are named, and several additional new species only known from material insufficient for formal description are listed. There are one unavailable nomen nudum and three doubtful names.
Diagnostic morphological traits of the recognised species are described and illustrated in detail where available of males, females, and eggs. Dichotomous keys to all species are provided. Mitochondrial DNA-data of 71 species-group taxa are available, which permits reliable association of sexes and minimises the risk of synonymies between species known from only one sex. Importantly, this includes DNA sequences from 50 holotypes. The DNA-data will also permit future monitoring with eDNA sequencing and identification of nymphs, which are probably important but so far unidentifiable bioindicators in streams.
The genus Neoperla occurs largely in the northern hemisphere and in Africa, where it is restricted to the Ethiopian region south of the Sahara but unknown from Madagascar. A single specimen not clearly distinct from a widespread mainland species was taken on Comoro Island. All African species are endemic, but one African species group has a few outlying members in Asia. The Asian N. montivaga-group is not known from Africa.
The following new species are described and named: N. aethiopica n. sp., N. amoena n. sp., N. angolana n. sp., N. bareensis n. sp., N. bella n. sp., N. benti n. sp., N. beta n. sp., N. bipolaris n. sp., N. biserrata n. sp., N. brachyphallus n. sp., N. caeleps n. sp., N. cataractae n. sp., N. claviger n. sp., N. coffea n. sp., N. costata n. sp., N. crenulata n. sp., N. crustata n. sp., N. decorata n. sp., N. dianae n. sp., N. dolium n. sp., N. dundoana n. sp., N. duodeviginti n. sp., N. erinaceus n. sp., N. excavata n. sp., N. filamentosa n. sp., N. funiculata n. sp., N. gibbosa n. sp., N. gordius n. sp., N. heideae n. sp., N. ivanloebli n. sp., N. juxtadidita n. sp., N. kalengonis n. sp., N. larvata n. sp., N. lineata n. sp., N. luhohonis n. sp., N. massevensis n. sp., N. multiserrata n. sp., N. muyukae n. sp., N. nichollsi n. sp., N. occulta n. sp., N. orthonema n. sp., N. pallidogigas n. sp., N. panafricana n. sp., N. pickeri n. sp., N. pilulifera n. sp., N. pirus n. sp., N. planidorsum n. sp., N. plicata n. sp., N. proxima n. sp., N. pusilla n. sp., N. rostrata n. sp., N. sambarua n. sp., N. sassandrae n. sp., N. schuelei n. sp., N. serrula n. sp., N. simplex n. sp., N. socia n. sp., N. sorella n. sp., N. spaghetti n. sp., N. spectabilis n. sp., N. spironema n. sp., N. tangana n. sp., N. tansanica n. sp., N. usambara n. sp., and N. vicina n. sp..
Uncertainty Quantification of Deep Learning–Based Statistical Downscaling of Climatic Parameters
Authors: Nourani Vahid , Khodkar Kasra , Baghanam Aida Hosseini , Kantoush Sameh Ahmed , Demir Ibrahim
Published: July 2023

This study investigated the uncertainty involved in statistical downscaling of hydroclimatic time series obtained by artificial neural networks (ANNs). Phase 6 of the Coupled Model Intercomparison Project (CMIP6) general circulation model (GCM) Canadian Earth System Model, version 5 (CanESM5), was used as large-scale predictor data for downscaling temperature and precipitation parameters. Two ANNs, feed forward and long short-term memory (LSTM), were utilized for statistical downscaling. To quantify the uncertainty of downscaling, prediction intervals (PIs) were estimated via the lower upper bound estimation (LUBE) method. To assess performance of proposed models in different climate regimes, data from the Tabriz and Rasht stations in Iran were employed. The calibrated models via historical GCM data were used for future projections via the high-forcing and fossil fuel–driven development scenario shared socioeconomic pathway (SSP) 5-8.5. Projections were compared with the Canadian Regional Climate Model 4 (Can-RCM4) projections via the same scenario. Results indicated that both LSTM-based point predictions and PIs are more accurate than the feedforward neural network (FFNN)-based predictions, with an average of 55% higher Nash–Sutcliffe efficiency (NSE) for point predictions and 25% lower coverage width criterion (CWC) for PIs. Projections suggested that Tabriz is going to experience a warmer climate with an increase in average temperature of 2° and 5°C for near and far futures, respectively, and a drier climate with a 20% decrease in precipitation until 2100. Future projections for the Rasht station, however, suggested a more uniform climate with less seasonal variability. Average precipitation will increase by up to 25% and 70% until near and far future periods, respectively. Ultimately, point predictions show that the average temperature in Rasht will increase by 1°C until the near future and then be a constant average temperature until the far future.

Significance Statement

The downscaling of hydroclimatic parameters is subject to uncertainty. The best way is to provide an area with the highest contingency of them as a prediction interval. The reduction width of such an interval leads to increased confidence in explaining and predicting these processes. We proposed and applied a deep learning–based machine learning method for both point prediction and prediction interval estimation of temperature and precipitation parameters for the future over two different climatic regions. The results show the superiority of such a machine learning–based prediction interval estimation for quantification of the downscaling uncertainty.
Serial Changes in Parotid Gland Volume and Symptoms After Radiation Therapy in Oropharyngeal Cancer
Authors: Jun Hyun Woong , Song Chang Myeon , Park Hae Jin , Ji Yong Bae , Tae Kyung
Published: July 2023

Objective: To evaluate the serial changes in the volume of the parotid gland and clinical symptoms after a course of radiation therapy (RT) in patients with oropharyngeal cancer. Methods: A total of 33 patients who were diagnosed with oropharyngeal cancer and had been treated with RT or concurrent chemoradiation therapy were evaluated. Parotid gland volumes were measured serially by head and neck computed tomography with contrast-enhanced images before RT, and 6 months, 1 year, and 2 years after RT. Patients also filled out EORTC (European Organization for the Research and Treatment of Cancer) QLQ-C30 questionnaires on the quality of life (QOL) at the same time. This questionnaire included questions about salivary gland function: dry mouth, sticky saliva, and taste disorder. Higher scores on EORTC questionnaire translates to worse QOL. Results: All patients received more than 60 Gy irradiation in total. The mean volume of parotid gland decreased from 23.30 mL before RT to 15.80 mL, 15.93 mL, and 16.67 mL after 6 months, 1 year, and 2 years, respectively ( P < 0.001 between pre-RT and all other 3 periods). The scores on the QOL questionnaire were higher (worsened QOL) at all 3 times after radiation than in the pre-RT period. The mean score of QOL increased from pre-RT to 2 years post-RT: “dry mouth” from 1.65 to 2.70, “sticky saliva” from 1.19 to 2.00, and “taste disorder” from 1.12 to 1.94. All 3 of these parameters were correlated with the volume of the parotid gland ( P < 0.005 each). Conclusions: The volume of the parotid gland decreases significantly after RT for oropharyngeal cancer and does not recover significantly for at least 2 years. There was a significant correlation between decreased parotid volume and a lower QOL involving salivation.
Surgical Techniques and Functional Reconstruction for Complex Tenosynovial Giant Cell Tumor of Temporal Bone and Middle Skull Base
Authors: Mei Honglin , Tian Cirong , Lin Hailiang , Lu Xiaoling , Ni Yusu
Published: July 2023

Objectives: Temporal tenosynovial giant cell tumors (TGCTs) are often large and have invaded the middle skull base. It is difficult to protect the important neurovascular structures around the tumor and perform functional reconstruction on the basis of complete tumor resection. This study aimed at analyzing the surgical techniques and functional reconstruction during the operation of complex TGCT of temporal bone and middle skull base. Methods: Five patients with pathologically confirmed TGCT of different complex types in the temporal bone and middle skull base were treated in our hospital from December 2020 to February 2023. We collected and retrospectively analyzed their medical records, including medical imaging, surgical procedures, and follow-up data. Results: The tumors invaded beyond the temporal bone and destroyed the middle skull base in all cases, involving the intracranial space and other important neurovascular structures. The internal carotid artery, infratemporal fossa, pterygopalatine fossa, and parapharyngeal space were also involved in 1 case. All the patients’ tumors were completely removed, and the operations were performed mainly via modified infratemporal fossa approach, or combined with expanded middle cranial fossa approach. All cases with temporal and skull base destruction were repaired using the temporalis muscle flap with no occurrence of intracranial complications. The dura mater, condyle of temporomandibular joint, facial nerve, and internal carotid artery were fully preserved. Normal maxillofacial morphology was also preserved. The air conductive hearing of 2 patients was preserved. Conclusions: We found and summarized some surgical techniques that can help safeguard the important structures around massive TGCTs of temporal bone and middle skull base, and reconstruct the defects after tumor resection. The techniques are effective and feasible.
Judders, Jabs, and Slips: Automation, Anomalies, Movements
Authors: Thornham Helen , Armitage Joanne
Published: July 2023

In May 2019, the “Automation and Me” event invited digital artists from around the world to come together in an unused shopping center unit in the heart of Leeds, United Kingdom, to critically respond to and explore the themes of automation, embodiment, and identity. In this article, we draw on Tobias Matzner’s argument that what algorithms are and do “emerges in a complex interplay of social practices, material properties, discourses, mathematical abstractions, code” to think through the event. In so doing, we explore a number of complex interplays as they were felt and lived by all of us who participated in it. We reflect on the experiences during the workshops through the particular framework of automation and feminist new materialism to explore how AI technologies and the complex surveillance of the smart environment replicated, on the one hand, existing and oppressive prejudice and, on the other hand, worked to constantly recalibrate and manipulate the context that humans and nonhumans navigate.
Unleashing the potential of chatbots in business: A bibliometric analysis
Authors: Ramya J B , Alur Sivakumar
Published: July 2023

Chatbots are increasingly becoming a fundamental part of social life. They allow us to communicate in more sophisticated ways using the internet. It is crucial to study chatbots to gain comprehensive insights into their developments, trends, impact, and advancement in the field. This paper presents a bibliometric analysis of chatbot research conducted using the R bibliometrix tool and SPAR-4 protocol. The analysis includes 805 scholarly articles published between 2005 and 2021, collected from the Web of Science and Scopus databases. A conceptual, intellectual, and social structure analysis was conducted to analyse impactful authors, journals, and documents. The study reveals that adoption of chatbots in business accelerated during the pandemic period. The findings also highlight a plethora of opportunities for research in areas like chatbot design cues, user experience, chatbots in collaboration with human agents, and chatbot ethics and privacy. This study's findings provide valuable insights for practitioners, policymakers, developers and researchers to understand the current state of the chatbot research landscape and to identify promising future research directions.