bluuebunny/crossref_metadata_2025 huggingface dataset:
2.5D mass spectrometry imaging of N-glycans in esophageal adenocarcinoma and precursor lesions
Authors: Vos D. R. N. , Meijer S. L. , Pouw R. E. , Ellis S. R. , Heeren R. M. A. , Balluff B.
Published: November 2022

Glycosylation plays an important role in the progression of esophageal adenocarcinoma (EAC). Being able to image these glycosylation changes directly in endoscopic resection specimens could provide useful insights into the molecular mechanisms of the disease progression and potential markers for EAC staging. For this purpose, both 3D and 2.5D matrix-assisted laser/desorption ionization (MALDI) mass spectrometry imaging (MSI) have been employed in this study to investigate glycosidase-cleaved N-glycans in a total of 24 formalin-fixed paraffin-embedded esophageal local excision specimens spanning all stages of disease progression, namely from non-dysplastic Barrett’s esophagus to metastatic EAC. 3D-MSI was first used to estimate the number of sections needed to sufficiently cover the molecular heterogeneity of each stage of progression. This analysis showed that a total of four sections out of 20 were sufficient. This subset of four sections was measured for all remaining specimens and is called 2.5D-MSI. Subsequent analyses of the 2.5D-MSI datasets revealed significant elevations of five high-mannose N-glycans (Man3, Man4, Man6, Man7, and Man8) in EAC and three complex (Hex6HexNAc5, Hex6HexNAc5NeuAc1, Hex7HexNAc6) N-glycans in metastatic EAC as compared to previous stages of the disease. The augmented levels of these glycans in EAC could be explained by publically available gene expression data of enzymes involved in glycan synthesis and processing. As the role of glycosylation is gaining more interest in MSI and cancer research, our results show the added value of combining localized N-glycan levels, as provided by MSI, with gene expression to gain a deeper understanding of the mechanisms behind N-glycan changes. This gives evidence at multiple levels that specific N-glycosylation plays an important role during progression of dysplasia to EAC and could play a role in patient surveillance.
Self-excited valve using a flat ring tube: Application to robotics
Authors: Nabae Hiroyuki , Kitamura Eigo
Published: November 2022

Complex and bulky driving systems are among the main issues for soft robots driven by pneumatic actuators. Self-excited oscillation is a promising approach for dealing with this problem: oscillatory actuation is generated from non-oscillatory input. However, small varieties of self-excited pneumatic actuators currently limit their applications. We present a simple, self-excited pneumatic valve that uses a flat ring tube (FRT), a device originally developed as a self-excited pneumatic actuator. First, we explore the driving principle of the self-excited valve and investigate the effect of the flow rate and FRT length on its driving frequency. Then, a locomotive robot containing the valve is demonstrated. The prototype succeeded in walking at 5.2 mm/s when the oscillation frequency of the valve was 1.5 Hz, showing the applicability of the proposed valve to soft robotics.
A rolled-up-based fabrication method of 3D helical microrobots
Authors: Wang Zihan , Mu Xueliang , Tan Liyuan , Zhong Yukun , Cheang U. Kei
Published: November 2022

While the potential of using helical microrobots for biomedical applications, such as cargo transport, drug delivery, and micromanipulation, had been demonstrated, the viability to use them for practical applications is hindered by the cost, speed, and repeatability of current fabrication techniques. Hence, this paper introduces a simple, low-cost, high-throughput manufacturing process for single nickel layer helical microrobots with consistent dimensions. Photolithography and electron-beam (e-beam) evaporation were used to fabricate 2D parallelogram patterns that were sequentially rolled up into helical microstructures through the swelling effect of a photoresist sacrificial layer. Helical parameters were controlled by adjusting the geometric parameters of parallelogram patterns. To validate the fabrication process and characterize the microrobots’ mobility, we characterized the structures and surface morphology of the microrobots using a scanning electron microscope and tested their steerability using feedback control, respectively. Finally, we conducted a benchmark comparison to demonstrate that the fabrication method can produce helical microrobots with swimming properties comparable to previously reported microrobots.
Enabling effective maize seed system in low-income countries of West Africa: Insights from Benin
Authors: Adigoun Rabiath F. R. , Houdegbe Aristide C. , Fassinou Hotegni Nicodème V. , Segnon Alcade C. , N'Danikou Sognigbe , Adjé Charlotte A. O. , Adadja Rigelle P. M. , Achigan-Dako Enoch G.
Published: November 2022

Introduction

Access to high-quality seeds remains a key constraint to the intensification of crop production in low-income countries. In this study, we analyzed maize seed production and distribution systems in Benin to identify leverage points for effective seed systems, a prerequisite for improving maize production.

Methods

Semi-structured interviews were conducted with 81 seed producers selected in seven municipalities across the three phytogeographical zones of Benin. Key informant interviews were also conducted with ten public and private stakeholders involved in maize seed systems in Benin.

Results and discussion

Findings showed that the legal and institutional frameworks governing seed systems in Benin were recently reinforced with a national seed policy, the creation and operationalization of the National Committee of Plant Seeds and the existence of regulations and rules on the production, quality control, certification, trade, and packaging of seeds. In addition, enabling conditions to facilitate the involvement of the private sector have been greatly improved with the revision of modalities for obtaining approval for the production and distribution of seeds in Benin. While the seed sector is improving and both public and private stakeholders are involved in maize seed production and distribution, synergies among stakeholders need to be strengthened. Strengthening business and marketing skills of seed producers through training and promoting the comparative advantages of improved seeds in increasing yield and production among maize farmers could be a promising avenue. Connecting seed producers with maize farmers' organizations coupled with ICT-based agro-advisories could boost the development of the maize seed sector, and ultimately the maize value chain.
Design and fabrication of modified bi-layer poly vinyl alcohol adhesive sealant film for preventing gastrointestinal leakage
Authors: Dorkhani Erfan , Noorafkan Yasmin , Asbagh Reza Akbari , Okhovat Maryam , Heirani-Tabasi Asieh , Ahmadi Tafti Seyed Mohsen
Published: November 2022

Graphical abstract

The design and fabrication of poly vinyl alcohol sealant film and evaluating efficacy of this novel patch for prevention of anastomosis leakage.
Knowledge, attitudes, and practices on camel respiratory diseases and conditions in Garissa and Isiolo, Kenya
Authors: Othieno Joseph , Njagi Obadiah , Masika Sophie , Apamaku Michael , Tenge Evans , Mwasa Bridgit , Kimondo Peter , Gardner Emma , Von Dobschuetz Sophie , Muriira Joseph , Adul Ben , Mwongela Lawrence , Hambe Haret A. , Nyariki Thomas , Fasina Folorunso O.
Published: November 2022

Background

Livestock farmers' attitudes, practices, and behaviors are major factors in infection prevention and control of animal diseases. Kenya has the fourth largest global camel population, and the industry has grown over the last two decades, transforming beyond the traditional camel-keeping areas to include peri-urban camel trade and value chain growth. The dromedary camel is resilient, and it is a preferred species in the arid and semi-arid areas (ASALs) of Kenya. However, it still faces many health and production challenges; to identify infection drivers and risky behaviors for camel respiratory illnesses and conditions in Kenya, we conducted a knowledge, attitudes, and practices (KAP) survey.

Method

Using a set of tools (questionnaires, key informant interviews, and focus group discussions), we interviewed camel owners, herders, agro-veterinary outlets, and other relevant value chain stakeholders in Garissa and Isiolo counties (n = 85). Data were analyzed using descriptive and analytic statistics.

Results

Most camel owners/herders are male and most are relatively uneducated (85.5%). The camels were used primarily for milk and meat production, income generation, and transport. Larger herd sizes (>30 camels) and owner/herder's lack of formal education are risk factors for owner-reported respiratory illnesses in camels. Major clinical signs of respiratory conditions were coughing (85.7%), nasal discharge (59.7%), and fever (23.4%). Diseases, lack of feeds, theft, and marketing challenges are the major constraints to camel production in Kenya. Owners-herders use drugs indiscriminately and this may contribute to antimicrobial resistance challenges.

Conclusion

Practitioners in the camel value chain want more commitment from the government and animal health officials on support services and access to veterinary services. Watering points, grazing areas, and marketing points are the primary areas for congregating camels and have a significant potential for disease spread. Kenya camels have a massive capacity for rural and ASALs' livelihoods transformation but the identified health challenges, and other issues must be addressed. Further studies on the Kenyan camels' respiratory microbial ecology are important to understand microbial risks and reduce the burden of zoonotic infections. Intensification of risk communication and community engagement, and messaging targeted at behavior change interventions should be directed at camel value chain actors.
Characterizing candidate decompression rates for hypobaric hypoxic stunning of pigs. Part 1: Reflexive behavior and physiological responses
Authors: Martin Jessica E. , Baxter Emma M. , Clarkson Jasmine M. , Farish Marianne , Clutton Richard E. , Greenhalgh Stephen N. , Gregson Rachael , McKeegan Dorothy E. F.
Published: November 2022

Alternatives to carbon dioxide (CO2) stunning for the commercial slaughter of pigs are urgently needed because there is robust evidence that exposing pigs to hypercapnic environments is associated with pain, fear, and distress. Hypobaric hypoxia (via gradual decompression, also known as Low Atmospheric Pressure Stunning or LAPS) has been validated in poultry as a humane option, but its potential to improve the welfare of pigs at slaughter is unknown. We investigated the potential of hypobaric hypoxia to reliably elicit a non-recovery state in anesthetized weaner-grower pigs within a commercially viable timeframe. We determined the effect of candidate decompression rates (40, 60, 80, 100 ms−1, at two cycle durations 480 s and 720 s) on a range of physiological and reflexive behavioral indicators of hypoxia and death. We found that the decompression rates tested caused a 100% death rate. As expected, the decompression rate had overarching effects on behavioral and physiological markers of hypoxia and death, with faster decompression rates resulting in shorter latencies to cardiac arrest and cessation of breathing. We observed a higher proportion of pigs displaying repeated and prolonged whole-body movements (likely indicative of convulsive activity) at higher frequencies when we applied the slowest decompression rate (40 ms−1) compared to all other rates. Since these responses may impact the carcass and meat quality, the slower rate of decompression (40 ms−1) should be excluded as a candidate decompression rate. Furthermore, given the marginal effects of decompression rate on physiological indicators of death and reflexive behavioral parameters, we also recommend that the fastest rate tested (100 ms−1) is excluded in further study on conscious pigs (to prevent conscious animals from being exposed to unnecessary faster decompression rates which may compromise animal welfare). This work represents a necessary proof of principle step and confirms the potential of gradual decompression for stunning purposes in pigs. Importantly, however, the data presented provide no information on the welfare outcomes associated with decompression in conscious pigs. Subsequent work should focus on the comprehensive welfare assessment of intermediate decompression rates to determine the potential of hypobaric hypoxia to provide a humane stunning method for pigs.
RAP44 phage integrase-guided 50K genomic island integration in Riemerella anatipestifer
Authors: Wang Ying , Deng Jianfeng , Ren Jianle , Liang Libin , Li Junping , Niu Sheng , Wu Xingchen , Zhao Yujun , Gao Shimin , Yan Fang , Liu Yuqing , Ma Haili , Tian Wen-xia , Yan Yi
Published: November 2022

Bacteriophages are viruses that infect bacteria. Bacteria and bacteriophages have been fighting for survival. Over time, the evolution of both populations has been affected. Pathogenic Flavobacteriaceae species including Riemerella anatipestifer mainly infects ducklings, geese, and turkeys. However, it does not infect humans, rats, or other mammals, and is a suitable and safe research object in the laboratory. Our previous study showed that there is a 10K genomic island in R. anatipestiferIn this study, we found another integrated 50K genomic islands and focused on the relationship between R. anatipestifer genomic islands and the RAP44 phage genome. The phage RAP44 genome was integrated into R. anatipestifer chromosome, and an evolutionary relationship was evident between them in our comparative analysis. Furthermore, the integrated defective RAP44 phage sequence had the function of integration, excision, and cyclization automatically. Integrases are important integration elements. The integrative function of integrase was verified in R. anatipestifer. The integrase with the attP site can be integrated stably at the attB locus of the R. anatipestifer genome. A recombinant strain can stably inherit and express the exogenous gene. By studying the integration between host bacterium and phage, we have provided evidence for the evolution of the genomes in R. anatipestifer.
3D Positioning Method for Pineapple Eyes Based on Multiangle Image Stereo-Matching
Authors: Liu Anwen , Xiang Yang , Li Yajun , Hu Zhengfang , Dai Xiufeng , Lei Xiangming , Tang Zhenhui
Published: November 2022

Currently, pineapple processing is a primarily manual task, with high labor costs and low operational efficiency. The ability to precisely detect and locate pineapple eyes is critical to achieving automated pineapple eye removal. In this paper, machine vision and automatic control technology are used to build a pineapple eye recognition and positioning test platform, using the YOLOv5l target detection algorithm to quickly identify pineapple eye images. A 3D localization algorithm based on multiangle image matching is used to obtain the 3D position information of pineapple eyes, and the CNC precision motion system is used to pierce the probe into each pineapple eye to verify the effect of the recognition and positioning algorithm. The recognition experimental results demonstrate that the mAP reached 98%, and the average time required to detect one pineapple eye image was 0.015 s. According to the probe test results, the average deviation between the actual center of the pineapple eye and the penetration position of the probe was 1.01 mm, the maximum was 2.17 mm, and the root mean square value was 1.09 mm, which meets the positioning accuracy requirements in actual pineapple eye-removal operations.
A Systematic Review and Meta-Analysis on the Role of Nutraceuticals in the Management of Neuropathic Pain in In Vivo Studies
Authors: Ilari Sara , Proietti Stefania , Russo Patrizia , Malafoglia Valentina , Gliozzi Micaela , Maiuolo Jessica , Oppedisano Francesca , Palma Ernesto , Tomino Carlo , Fini Massimo , Raffaeli William , Mollace Vincenzo , Bonassi Stefano , Muscoli Carolina
Published: November 2022

The control of neuropathic pain is a leading challenge in modern medicine. Traditional medicine has, for a long time, used natural compounds such as nutraceuticals for this purpose, and extensive evidence has supported their role in controlling oxidative stress and persistent pain-related inflammation. Nutraceuticals are natural products belonging to the food sector whose consumption could be related to physiological benefits. Indeed, they are used to improve health, prevent chronic diseases, and delay the aging process. Here, we report a systematic review and meta-analysis to provide a more comprehensive report on the use of nutraceuticals in neuropathic pain, including evaluating confounding factors. A search of the literature has been conducted on principal databases (PubMed, MEDLINE, EMBASE, and Web of Science) following the PRISMA statement, and we retrieved 484 articles, 12 of which were selected for the meta-analysis. The results showed that administration of natural drugs in animals with neuropathic pain led to a significant reduction in thermal hyperalgesia, measured in both the injured paw (SMD: 1.79; 95% CI: 1.41 to 2.17; p < 0.0001) and in the two paws (SMD: −1.74; 95% CI: −3.36 to −0.11; p = 0.036), as well as a reduction in mechanical allodynia and hyperalgesia (SMD: 1.95, 95% CI: 1.08 to 2.82; p < 0.001) when compared to controls. The results of the review indicate that nutraceutical compounds could be clinically relevant for managing persistent neuropathic pain.
Simulation on Unsteady Crosswind Forces of a Moving Train in a Three-Dimensional Stochastic Wind Field
Authors: Yao Zhiyong , Zhang Nan , Li Xiaoda , Liu Zongchao
Published: November 2022

Unsteady aerodynamic forces are significantly critical to the safety and stability of trains traveling in high winds. This paper describes a study into unsteady crosswind forces of a moving train subjected to a three-dimensional stochastic wind field with longitudinal, lateral, and vertical turbulences. Initially, a three-dimensional computational fluid dynamic (CFD) model is established to calculate the aerodynamic coefficient of a moving train, and then the wind velocity time histories at the position of the train are generated. Finally, the quasi-steady theory and weighting function method are used to model the unsteady crosswind forces of a moving train in a three-dimensional turbulence field. The results demonstrate that a generalized sine form is useful for predicting the aerodynamic coefficient that varies with the resultant wind yaw angle, and an adequate modeling of unsteady crosswind forces with complete wind turbulences can produce a greater force fluctuation and peak. Particularly when the flow direction of crosswind deviates from 90°, consideration of only a portion of the turbulence components may underestimate the dynamic response of trains.
The Effect of Hydrolysis on the Antioxidant Activity of Olive Mill Waste
Authors: Attard Karen , Oztop Mecit Halil , Lia Frederick
Published: November 2022

This study presents the effect of hydrolysis on the antioxidant activity of olive mill waste. The olive pomace samples were collected at different stages of maturity and were investigated for their phenolic content and antioxidant activity. Three different extraction procedures were employed, including methanolic maceration extraction and two hydrolysed procedures using 6 M HCL for acid hydrolysis and 10 M NaOH for alkaline hydrolysis. The total phenolic, flavonoid and ortho-diphenolic content, metal ion reducing activity, 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) and 2,2-Diphenyl-1-picrylhydrazyl Radical Scavenging, hydrogen peroxide and superoxide scavenging activity assays were determined for the different extracts. In this study, cultivar and maturation of olives was one of the factors that affected the phenolic content in the olive pomace samples. Results show that alkaline hydrolysis had the highest antioxidant activity with respect to total phenolic content, 2,2-Diphenyl-1-picrylhydrazyl scavenging activity, metal ion reducing activity and superoxide scavenging activity, whereas acid hydrolysis had the highest 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) scavenging activity. The correlation analysis carried out on the different phenolic classes revealed that the total phenolic, flavonoid and ortho-diphenolic content were correlated with metal ion reducing activity and Radical Scavenging activity.
Assessment of Concrete Strength Using the Combination of NDT—Review and Performance Analysis
Authors: Kouddane Bouchra , Sbartaï Zoubir Mehdi , Alwash Maitham , Ali-Benyahia Khoudja , Elachachi Sidi Mohammed , Lamdouar Nouzha , Kenai Said
Published: November 2022

This paper presents a review on combining NDT techniques, such as rebound hammer and ultrasonic pulse velocity, for assessing concrete compressive strength. These methods, though being favorably not invasive and easy to be extended to a larger number of elements, are affected by many contingency factors. The SonReb technique suggests combining the two methods to partially offset their low reliability if considered separately. For years, this concept was introduced in order to improve the evaluation compared with the use of one NDT. In order to combine the ultrasonic pulse velocity and rebound hammer, many empirical, multiparametric models were proposed in the literature as linear, power, exponential, or polynomial. However, the variety of these models emphasizes that they can give a correct strength prediction only for the particular cases that they are derived for. Therefore, to assess concrete on site, the strength should be predicted using a calibration procedure due to the variability of existing concrete mixes. This paper presents a brief outline of the key aspects of strength assessment, including the different approaches used to build the SonReb model and a calibration procedure for assessing concrete strength. A comparison study between the different approaches is proposed, and a performance analysis using Monte Carlo simulations is discussed. Finally, the estimation capacity of the existing model identification approaches is investigated, and the effect of the “trade-off” is analyzed for different random sampling with varying the number of cores.
The Occurrence and Bioremediation of Emerging Polyfluorinated Compounds in Water Bodies: A Mini Review
Authors: Khumalo Siphesihle Mangena , Bakare Babatunde Femi , Rathilal Sudesh
Published: November 2022

The occurrence and fate of polyfluorinated compounds (PFCs) in the aquatic environment resulting from anthropogenic activities has become an emerging issue of environmental chemistry. PFCs have been detected in drinking water samples, aquatic life, human tissue, and blood serum. This is attributed to their xenobiotic attributes making them environmentally persistent, bio-accumulative, and globally distributed in water receiving bodies, posing serious health problems to aquatic life and human health. This is ascribed to PFCs’ peculiar physicochemical properties of being hydrophobic and oleophobic and their removal process from wastewater streams is different from any other organic pollutants. Therefore, this review summarizes the environmental occurrence and recent developments on microbial degradation of the most detected PFCs, i.e., perfluorooctanoic acid (PFOA), and perfluorooctane sulfonic acid (PFOS) in water bodies. The available literature suggests that PFOA and PFOS are susceptible to biodegradation by Acidimicrobium sp. strain A6, Pseudomonas parafulva strain YAB1, Pseudomonas plecoglossicidia 2.4-D, and Pseudomonas aeruginosa strain HJ4. Moreover, the current study presents a summary on phytoremediation of PFOA and PFOS as a sustainable green technology. Despite the extensive work undertaken on bioremediation of PFOA and PFOS by biological processes, the available literature suggests that a lot of work still needs to be carried out aimed at investigating the biodegradation pathway of PFOA and PFOS by both microbial species and plants.
An Application of Rabotnov Functions on Certain Subclasses of Bi-Univalent Functions
Authors: Amourah Ala , Aldawish Ibtisam , Alhindi Khadeejah Rasheed , Frasin Basem Aref
Published: November 2022

In this study, a new class RΣμ(x,γ,α,δ,β) of bi-univalent functions studied by means of Gegenbauer polynomials (GP) with Rabotnov functions is introduced. The coefficient of the Taylor coefficients a2 and a3 and Fekete-Szegö problems for functions belonging to RΣμ(x,γ,α,δ,β) have been derived as well. Furthermore, a variety of new results will appear by considering the parameters in the main results.
Design of Cuboidal FeNi2S4-rGO-MWCNTs Composite for Lithium-Ion Battery Anode Showing Excellent Half and Full Cell Performances
Authors: Pramanik Atin , Chattopadhyay Shreyasi , De Goutam , Mahanty Sourindra
Published: November 2022

Ternary metal sulfides are projected as advanced lithium-ion battery (LIB) anodes due to their superior electronic conductivity and specific capacity compared to their respective oxide counterparts. Herein, a porous composite of cuboidal FeNi2S4 (FNS) with 2D reduced graphene oxide (rGO) and 1D multi-walled carbon nanotubes (MWCNTs) (composite name: FNS@GC) synthesised by an in-situ single-step hydrothermal process. The 1D/2D combined thin carbon coatings on the FeNi2S4 prevent aggregation during battery performance by increasing conductivity and resisting the volume changes at lithiation/de-lithiation processes. Consequently, the FNS@GC composite exhibits a commending electrochemical performance with a charge capacity of 797 mAh g−1 and a first cycle coulombic efficiency of ~67% with reversible capacity restoration property and excellent long-term cycling stability. Furthermore, FNS@GC//LiFePO4 full cell reveals its practical applicability as a LIB anode with a reversible capacity of 77 mAh g−1 at 50 mA g−1 current density.
Forensic Application of Stable Isotopes to Distinguish between Wild and Captive Turtles
Authors: Hopkins John , Frederick Cheryl , Yorks Derek , Pollock Erik , Chatfield Matthew
Published: November 2022

Wildlife traffickers often claim that confiscated animals were captive-bred rather than wild-caught to launder wild animals and escape prosecution. We used stable isotopes (δ13C and δ15N) derived from the claw tips of wild wood turtles from Maine and captive wood turtles throughout the eastern U.S. to develop a predictive model used to classify confiscated wood turtles as wild or captive. We found that the claw tips of wild and captive wood turtles (Glyptemys insculpta) were isotopically distinct. Captive turtles had significantly higher δ13C and δ15N values than wild turtles. Our model correctly classified all wild turtles as wild (100%) and nearly all captive turtles as captive (94%). All but two of the 71 turtles tested were successfully predicted as wild or captive (97.2% accuracy), yielding a misclassification rate of 2.8%. In addition to our model being useful to law enforcement in Maine, we aim to develop a multi-species model to assist conservation law enforcement efforts to curb illegal turtle trafficking from locations across the eastern United States and Canada.
The Use of Mesenchymal Stem Cells in the Complex Treatment of Kidney Tuberculosis (Experimental Study)
Authors: Muraviov Alexander N. , Vinogradova Tatiana I. , Remezova Anna N. , Ariel Boris M. , Gorelova Anna A. , Orlova Nadezhda V. , Yudintceva Natalia M. , Esmedliaeva Diljara S. , Dyakova Marina E. , Dogonadze Marine Z. , Zabolotnykh Natalia V. , Garapach Irina A. , Maslak Olga S. , Kirillov Yuri A. , Timofeev Sergei E. , Krylova Yulia S. , Yablonskiy Petr K.
Published: November 2022

In recent years, the application of mesenchymal stem cells (MSCs) has been recognized as a promising method for treatment of different diseases associated with inflammation and sclerosis, which include nephrotuberculosis. The aim of our study is to investigate the effectiveness of MSCs in the complex therapy of experimental rabbit kidney tuberculosis and to evaluate the effect of cell therapy on the reparative processes. Methods: To simulate kidney tuberculosis, a suspension of the standard strain Mycobacterium tuberculosis H37Rv (106 CFU) was used, which was injected into the cortical layer of the lower pole parenchyma of the left kidney under ultrasound control in rabbits. Anti-tuberculosis therapy (aTBT) was started on the 18th day after infection. MSCs (5 × 107 cells) were transplanted intravenously after the start of aTBT. Results: 2.5 months after infection, all animals showed renal failure. Conducted aTBT significantly reduced the level of albumin, ceruloplasmin, elastase and the severity of disorders in the proteinase/inhibitor system and increased the productive nature of inflammation. A month after MSC transplantation, the level of inflammatory reaction activity proteins decreased, the area of specific and destructive inflammation in kidneys decreased and the formation of mature connective tissue was noted, which indicates the reparative reaction activation.
A Systematic Review of the Evidence of Hematopoietic Stem Cell Differentiation to Fibroblasts
Authors: Smilde Bernard J. , Botman Esmée , de Vries Teun J. , de Vries Ralph , Micha Dimitra , Schoenmaker Ton , Janssen Jeroen J. W. M. , Eekhoff Elisabeth M. W.
Published: November 2022

Fibroblasts have an important role in the maintenance of the extracellular matrix of connective tissues by producing and remodelling extracellular matrix proteins. They are indispensable for physiological processes, and as such also associate with many pathological conditions. In recent years, a number of studies have identified donor-derived fibroblasts in various tissues of bone marrow transplant recipients, while others could not replicate these findings. In this systematic review, we provide an overview of the current literature regarding the differentiation of hematopoietic stem cells into fibroblasts in various tissues. PubMed, Embase, and Web of Science (Core Collection) were systematically searched for original articles concerning fibroblast origin after hematopoietic stem cell transplantation in collaboration with a medical information specialist. Our search found 5421 studies, of which 151 were analysed for full-text analysis by two authors independently, resulting in the inclusion of 104 studies. Only studies in animals and humans, in which at least one marker was used for fibroblast identification, were included. The results were described per organ of fibroblast engraftment. We show that nearly all mouse and human organs show evidence of fibroblasts of hematopoietic stem cell transfer origin. Despite significant heterogeneity in the included studies, most demonstrate a significant presence of fibroblasts of hematopoietic lineage in non-hematopoietic tissues. This presence appears to increase after the occurrence of tissue damage.
Hyperuricemia and Endothelial Function: Is It a Simple Association or Do Gender Differences Play a Role in This Binomial?
Authors: Ciarambino Tiziana , Crispino Pietro , Giordano Mauro
Published: November 2022

The endothelium plays a fundamental role in the biological processes that ensure physiological vessel integrity, synthesizing numerous substances that are capable of modulating the tone of vessels, inflammation and the immune system, and platelet function. Endothelial dysfunction refers to an anomaly that develops at the level of the tunica that lines the internal surface of arterial and venous vessels, or, more precisely, an alteration to normal endothelial function, which involves the loss of some structural and/or functional characteristics. Studies on sex differences in endothelial function are conflicting, with some showing an earlier decline in endothelial function in men compared to women, while others show a similar age of onset between the sexes. Since increased cardiovascular risk coincides with menopause, female hormones, particularly estrogen, are generally believed to be cardioprotective. Furthermore, it is often proposed that androgens are harmful. In truth, these relationships are more complex than one might think and are not just dependent on fluctuations in circulating hormones. An increase in serum uric acid is widely regarded as a possible risk factor for cardiovascular disease; however, its role in the occurrence of endothelial dysfunction has not yet been elucidated. Several studies in the literature have evaluated sex-related differences in the association between elevated uric acid levels and cardiovascular events, with conflicting results. The association between uric acid and cardiovascular disease is still controversial, and it is not yet clear how gender differences affect the serum concentration of these substances. This review was primarily aimed at clarifying the effects of uric acid at the level of the vascular endothelium and describing how it could theoretically cause damage to endothelial integrity. The second aim was to determine if there are gender differences in uric acid metabolism and how these differences interact with the vascular endothelium.
DNA and mRNA Vaccines for Chronic Viral Infections and Cancer: Rationale, Mechanisms, and Progress
Authors: Liu Margaret A.
Published: November 2022

Interest in the capabilities of nucleic acid vaccines, (DNA and mRNA vaccines) for both prophylactic and therapeutic uses have greatly increased following the successful deployment of two mRNA and, on a more limited scale, one DNA vaccine for COVID-19. In addition to targeting other pathogens for prophylactic vaccines, efforts are also being made towards using them for therapies for chronic infections and cancer. An examination of past and current successes for such therapies using other technologies with an emphasis on the immunological mechanisms will be provided followed by an assessment of the relevant characteristics of DNA and mRNA vaccines to predict their utility for therapies for chronic viral infections and cancer. Efforts and progress for these targets will be described.
Improving ERPs Integration in Organization: An EOS-Based GreneOS Implementation
Authors: Rahme Joseph , Masimukku Bharat , Daclin Nicolas , Zacharewicz Gregory
Published: November 2022

Current ERPs are still limited by cost, customization, implementation time, and interoperability with other systems. Even if cloud-based ERPs attempt to overcome these limits, they do not completely answer all of them. Based on that postulate about recent ERPs, a conceptual architecture, technical architecture, and implementation architecture of an Enterprise Operating System (EOS) have been designed and proposed to address the services and functionality needed by Enterprise 4.0. This conceptual architecture describes the essential functions required in the EOS, while the technical architecture shows how these tasks cooperate to achieve the mission of the EOS. Among some implementation architectures proposed that benefited from the innovation and concept of the EOS, GreneOS has proposed a technical architecture motivated by EOS concepts. The purpose of this paper is to discuss the current interest, complementarity, and limitation of both the EOS conceptual architecture and its implementation into GreneOS to propose perspectives for the future developments of the EOS.
Systematic Trends in Hybrid-DFT Computations of BaTiO3/SrTiO3, PbTiO3/SrTiO3 and PbZrO3/SrZrO3 (001) Hetero Structures
Authors: Eglitis Roberts I. , Piskunov Sergei , Popov Anatoli I. , Purans Juris , Bocharov Dmitry , Jia Ran
Published: November 2022

We performed predictive hybrid-DFT computations for PbTiO3, BaTiO3, SrTiO3, PbZrO3 and SrZrO3 (001) surfaces, as well as their BaTiO3/SrTiO3, PbTiO3/SrTiO3 and PbZrO3/SrZrO3 (001) heterostructures. According to our hybrid-DFT computations for BO2 and AO-terminated ABO3 solid (001) surfaces, in most cases, the upper layer ions relax inwards, whereas the second layer ions shift upwards. Our hybrid-DFT computed surface rumpling s for the BO2-terminated ABO3 perovskite (001) surfaces almost always is positive and is in a fair agreement with the available LEED and RHEED experiments. Computed B-O atom chemical bond population values in the ABO3 perovskite bulk are enhanced on its BO2-terminated (001) surfaces. Computed surface energies for BO2 and AO-terminated ABO3 perovskite (001) surfaces are comparable; thus, both (001) surface terminations may co-exist. Our computed ABO3 perovskite bulk Γ-Γ band gaps are in fair agreement with available experimental data. BO2 and AO-terminated (001) surface Γ-Γ band gaps are always reduced with regard to the respective bulk band gaps. For our computed BTO/STO and PTO/STO (001) interfaces, the average augmented upper-layer atom relaxation magnitudes increased by the number of augmented BTO or PTO (001) layers and always were stronger for TiO2-terminated than for BaO or PbO-terminated upper layers. Our B3PW concluded that BTO/STO, as well as SZO/PZO (001) interface Γ-Γ band gaps, very strongly depends on the upper augmented layer BO2 or AO-termination but considerably less so on the number of augmented (001) layers.
Buck-Boost DC-DC Converters for Fuel Cell Applications in DC Microgrids—State-of-the-Art
Authors: Andrade Pedro , Alcaso Adérito Neto , Bento Fernando , Marques Cardoso Antonio J.
Published: November 2022

The use of fuel cells in DC microgrids has been receiving a lot of attention from researchers and industry since both technologies can deliver clean energy with little to no environmental impact. To effectively integrate fuel cells in DC microgrids, a power converter that can equate the fuel cell’s voltage with the DC microgrid’s reference voltage is required. Based on the typical output voltages of fuel cells, buck-boost topologies are commonly used in this type of application. A variety of DC-DC buck-boost topologies, showing distinctive merits and drawbacks, are available in the literature. Therefore, this paper compiles, compares and describes different DC-DC buck-boost topologies that have been introduced in the literature over the past few years. Additionally, some design considerations are addressed, and future work is proposed.
Research Progress of Fermented Functional Foods and Protein Factory-Microbial Fermentation Technology
Authors: Sun Wenli , Shahrajabian Mohamad Hesam , Lin Min
Published: November 2022

Fermentation has been used for ages as a safe technique for food preservation, and it uses minimal resources. Fermentation is related to a wide range of catabolic biochemical procedures in both eukaryotes and prokaryotes. Yeasts are eukaryotes; they can use oxygen while also having the ability to live without oxygen. The lactate fermentation process consists of glycolysis and some alternative steps. A review of the literature was done using keywords in main indexing systems, including PubMed/MEDLINE, Scopus, the search engine of the Institute for Scientific Web of Science and Google Scholar. The keywords reviewed were fermentation technologies, protein mass expression, health benefits of functional foods, microbial fermentation technology, anaerobic respiration, fermentation in eukaryotes, fermentation in prokaryotes, solid state fermentation and submerged fermentation. This research was carried out to highlight the importance of fermentation technology and to introduce and survey the technology and its relationship with functional foods. Research progress in the area of protein factory-microbial fermentation technology was also investigated and inspected.
Chemical and Sensory Characteristics of Different Red Grapes Grown in Xinjiang, China: Insights into Wines Composition
Authors: Miao Yuanyuan , Wang Huan , Xu Xiaoyu , Ye Piping , Wu Huimin , Zhao Ruirui , Shi Xuewei , Cai Fei
Published: November 2022

Globally, the red wine market experienced a rapid growth in the last decade, due to the superior colour, taste, and nutritional quality. The red grapes used for vinification have individual characteristics varying within the regional environment. In this study, the quality of seven grape cultivars, including Marselan, Yan 73, Muscat Hamburg, Kadarka, Merlot, Cabernet Sauvignon, and Crimpose, and their corresponding wines, were investigated based on high-performance liquid chromatography and headspace solid-phase microextraction coupled to gas chromatography–mass spectrometry. These techniques were performed to analyze the chemical compositions and volatile compounds of the tested samples, respectively. The results showed that tartaric acid (29.96% to 73.45%) and rutin (12.53% to 56.54%) were the dominant organic acid and phenolic compounds in grapes, respectively. Higher concentrations of organic acids and phenolic compounds, and the types of volatile compounds, were observed to be highest in the Cabernet Sauvignon grape. The antioxidant activity of wines ranged from 6.74 to 102.68 mmol TE/L, and Yan 73 wine had the highest antioxidant activity. A total of 69 volatile compounds consisting of 17 alcohols, 26 esters, 5 aldehydes, 9 acids, 7 ketones, and 5 other volatile compounds were identified in all tested wines, and 11 important aroma active substances (odor activity value > 1) were selected, consisting of β-ionone, phenethyl acetate, geranyl acetate, ethyl 9-decenoate, ethyl caprate, ethyl pelargonate, decanal, ethyl caprylate, 6-methyl-5-hepten-2-one, methyl 2-hexenoate, and ethyl hexanoate, which endow wines with a unique aroma. This work clearly describes the chemical and sensory characteristics of seven red grape cultivars in Xinjiang of China and provides diversity options for cultivars for winemaking.
Influence of Thermal Regimes on the Relationship between Parasitic Load and Body Condition in European Sardine along the Catalan Coast
Authors: Frigola-Tepe Xènia , Caballero-Huertas Marta , Viñas Jordi , Muñoz Marta
Published: November 2022

The small pelagic European sardine presents high commercial and ecological values. Due to its cold-temperate water affinity, stocks are affected by global warming. Water temperature rise may change primary productivity patterns, negatively affecting fish condition and increasing parasite incidence. In this context, sardine health status was evaluated through the annual cycle on the Catalan Coast using thermal regimes comparison. Morphogravimetric parameters, sex and gonadal stages were assessed; infection by nematodes was characterised, and body condition was estimated by the Le Cren Factor and lipid content measured using a fish fat meter. Significant statistical differences were observed in spawning dynamics, body condition, and parasite infection between thermal regimes. Sardines from the colder north area had better condition and an earlier spawning, with lower parasite incidence (in terms of total prevalence, mean intensity and abundance) than those from the southern coast. Hysterothylacium spp. was the most abundant nematode, while Anisakis spp. prevalence was null in the two locations. Seasonal differences in nematode load were observed along the Catalan Coast, with lower prevalence during the summer and higher in winter-spring. Although previous studies have underestimated parasite influence on sardine health status, parasite abundance and sardine condition were negatively correlated. Seawater temperature and primary productivity are the proposed factors promoting differentiation in nematode infection and fish condition throughout the annual cycle and between locations.
Identification and Evolution of a Natural Tetr Protein Based on Molecular Docking and Development of a Fluorescence Polari-Zation Assay for Multi-Detection of 10 Tetracyclines in Milk
Authors: Xia Wanqiu , Liu Jing , Wang Jianping
Published: November 2022

In this study, the identity of our recently produced natural TetR protein was identified by using the LC-ESI-MS/MS technique, and its recognition mechanisms, including the binding pocket, contact amino acids, intermolecular forces, binding sites, binding energies, and affinities for 10 tetracycline drugs were studied. Then, it was evolved by site-mutagenesis of an amino acid to produce a mutant, and a fluorescence polarization assay was developed to detect the 10 drugs in milk. The sensitivities for the 10 drugs were improved with IC50 values decreasing from 30.8–80.1 ng/mL to 15.5–55.2 ng/mL, and the limits of detection were in the range of 0.4–1.5 ng/mL. Furthermore, it was found that the binding affinity for a drug was the critical factor determining its sensitivity, and the binding energy showed little influence. This is the first study reporting the recognition mechanisms of a natural TetR protein for tetracyclines and the development of a fluorescence polarization assay for the detection of tetracyclines residues in food samples.
A Coordinated Analysis of Physical Reactivity to Daily Stressors: Age and Proactive Coping Matter
Authors: Neupert Shevaun D. , Smith Emily L. , Schriefer Margaret L.
Published: November 2022

Proactive coping involves efforts to prepare for future stressors and may have implications for physical responses to stress. We examined age differences in physical reactivity to daily stressors moderated by proactive coping in a coordinated analysis across two separate daily diary studies. Study 1 included data from 116 older (age range 60–90) and 107 younger (age range 18–36) adults on daily stressors and physical health symptoms for 8 consecutive days. Study 2 included data from 140 adults (age range 19–86) on daily stressors and self-rated physical health for 29 consecutive days. Participants in both studies reported on their proactive coping on the first day of the study. Physical reactivity was indexed via lagged multilevel models as increases in daily physical symptoms in Study 1 and decreases in daily physical health in Study 2 with corresponding increases in daily stressors. Results indicated that in both studies, younger adults with low proactive coping were more physically reactive to daily stressors compared to younger adults with high proactive coping. Proactive coping was associated with reduced physical reactivity to daily stressors among younger adults, consistent with the characterization of a high degree of control and ample opportunities at earlier phases of adulthood which are critical for accumulating resources to proactively cope.
Auto-Continuous Synthesis of Robust and Hydrophobic Silica Aerogel Microspheres from Low-Cost Aqueous Sodium Silicate for Fast Dynamic Organics Removal
Authors: Sun Ziqian , Zhao Zhiyang , Kong Yong , Ren Jian , Jiang Xing , Shen Xiaodong
Published: November 2022

An efficient auto-continuous globing process was developed with a self-built apparatus to synthesize pure silica aerogel microspheres (PSAMs) using sodium silicate as a precursor and water as a solvent. A hydrophobic silica aerogel microsphere (HSAM) was obtained by methyl grafting. A reinforced silica aerogel microsphere (RSAM) was prepared by polymer cross-linking on the framework of the silica gel. The pH value of the reaction system and the temperature of the coagulating bath were critical to form perfect SAMs with a diameter of 3.0 ± 0.2 mm. The grafted methyl groups are thermally stable up to 400 °C. Polymer cross-linking increased the strength significantly, owing to the polymer coating on the framework of silica aerogel. The pore volumes of HSAM (6.44 cm3/g) and RSAM (3.17 cm3/g) were much higher than their state-of-the-art counterparts. Their specific surface areas were also at a high level. The HSAM and RSAM showed high organic sorption capacities, i.e., 17.9 g/g of pump oil, 11.8 g/g of hexane, and 22.2 mg/g of 10 mg/L methyl orange. The novel preparation method was facile, cost-effective, safe, and eco-friendly, and the resulting SAM sorbents were exceptional in capacity, dynamics, regenerability, and stability.