bluuebunny/crossref_metadata_2025 huggingface dataset:
Research on Rotor Loss and Rotor Structure Optimization of Rare Earth Permanent Magnet Synchronous Motors in the Weak Field Area
Authors: Yang Guohui , Wu Hao , Wang Danyang , Zhang Peng , Jiang Shuaiqi , Zhang Yang
Published: November 2023

The rotor loss of rare earth permanent magnet synchronous motor (PMSM) affects the efficiency and reliability of the motor and is an important research direction of rare earth permanent magnet synchronous motors. In this paper, the influence of rotational speed on the loss of the rotor core and the permanent magnet is analyzed through the analysis of the motor magnetic field and rotor loss calculation. Specifically, with the increase in rotational speed, the weak magnetic current increases, the amplitude of flux density change in the rotor core decreases, and the loss of the rotor core also decreases. The greater the influence of the harmonic current magnetic field on the outside of the rotor, the greater the loss. The core loss on the rotor is mainly distributed on the outside of the rotor. For further engineering applications, this paper analyzes the loss composition of three kinds of motors with different rotor structures and judges the optimal scheme based on the minimum loss. Finally, the correctness of this research is verified by comparing the experimental and simulation results of two kinds of motors with different rotor structures. The research content of this paper can provide a reference for the loss analysis and optimization and rotor structure selection of rare earth permanent magnet synchronous motor rotor design.
Reactive Power Dispatch Algorithm for a Reduction in Power Losses in Offshore Wind Farms
Authors: Song Seung-Ho , Tae Gyo-Won , Lim Alexandr , Kim Ye-Chan
Published: November 2023

This paper presents a groundbreaking power distribution technique that focuses on the loss rate of individual wind turbines. Distinct from conventional methods, our strategy prioritizes seamless integration and adaptability within wind farm management systems. By evaluating power losses in specific branches of a wind farm, our approach enhances overall performance by strategically allocating reactive power to reduce cumulative losses. When compared to traditional uniform distribution and Particle Swarm Optimization (PSO) methods, our innovative approach stands out for its superior efficiency and adaptability. Comprehensive simulations underline the strengths and weaknesses of prevailing methods and underscore the superior efficacy of our proposed technique.
Optimisation Design of a Low-Frequency Eddy Current Rail Heater
Authors: Canova Aldo , Tartaglia Michele , Quercio Michele
Published: November 2023

The aim of the work is the optimisation of a rail heater, constituted by a magnetic core supplied by a sinusoidal current, which induces an eddy current in the rail. Optimisation parameters are electrical and geometrical quantities: supply frequency, voltage amplitude, airgaps, and core shape, while objectives are power transferred to the rail, absorbed current, and power distribution index. Optimisation is performed by an accurate field analysis, provided by the finite element method (FEM), coupled to an automated multiobjective procedure based on fuzzy logic. Particular care has been devoted to the FEM model in order to take into account important phenomena as non-linearity magnetic behaviour and non-uniform distribution of current in the rail caused by eddy currents.
Which Policies and Factors Drive Electric Vehicle Use in Nepal?
Authors: Ghimire Laxman Prasad , Kim Yeonbae , Dhakal Nawa Raj
Published: November 2023

Electric vehicles (EVs) offer a viable technological solution for mitigating greenhouse gas emissions in the transportation industry, addressing pressing societal concerns regarding climate change, air pollution, and sustainable energy consumption. To effectively promote widespread adoption of EVs, it is crucial to understand consumer preferences and evaluate market dynamics. In Nepal, where proven fossil fuel reserves are absent, the government is actively working towards accelerating EV adoption, leveraging the nation’s significant hydroelectric power generation potential to fulfill EVs’ charging demands. To gain insight into consumer preferences and evaluate market dynamics regarding EVs in Nepal, this study employs a comprehensive approach. Stated preference data are collected through a meticulously designed survey, and sophisticated analytical techniques, namely, the mixed logit model and latent class model, are applied for estimation purposes. The results of this study show that potential EV consumers with small family sizes, lower monthly travel distances, heightened environmental awareness, and substantial knowledge about electric vehicles are more inclined to embrace EV technology. Notably, the study highlights that a reduction in the purchase price exerts the most significant influence on increasing consumers’ likelihood of adopting battery electric vehicles (BEVs) and plug-in hybrid electric vehicles (PHEVs). Market simulation results suggest that a policy mix scenario, encompassing a combination of supportive measures, proves more effective in promoting EV adoption compared to relying on single policy initiatives. Furthermore, through latent class estimation, the study identifies three distinct classes of consumers within Nepal, each exhibiting significant variations in preferences. Recognizing and addressing these variations within policy frameworks is crucial for the successful promotion and widespread acceptance of EVs in Nepal.
Managing Earthquake Debris: Environmental Issues, Health Impacts, and Risk Reduction Measures
Authors: Mavroulis Spyridon , Mavrouli Maria , Lekkas Efthymis , Tsakris Athanasios
Published: November 2023

Earthquakes have the potential to cause severe and widespread structural damage to buildings and infrastructure in the affected area. Earthquake debris mainly results from building collapses during intense ground motion and the emergency demolition of damaged and unstable buildings following a devastating earthquake. Debris management constitutes a major challenge that must be met by all those participating in disaster management as it poses threats to both the natural environment and public health in an earthquake-affected area. This narrative review examines the hazards that arise throughout the early phases of debris removal, when personnel operate in disaster-affected areas, to the last steps of sorting and disposal. Furthermore, emphasis is also given to the environmental impact caused by unregulated debris disposal on natural habitats that are highly sensitive and susceptible to hazardous substances and materials found in the debris. In the same framework, measures are proposed for alleviating the negative impacts of debris management on the well-being of all individuals involved, including workers, volunteers, and the local community, as well as the surrounding natural environment, encompassing soil, surface and groundwater, as well as air quality.
Oxidative Phosphorylation for Aerobic Survival, but Not for Growth: The Peculiar ‘Make-Accumulate-Consume’ Strategy in Zymomonas mobilis
Authors: Strazdina Inese , Bikerniece Mara , Paegle Evelina Rezija , Shvirksts Karlis , Grube Mara , Lasa Zane , Rutkis Reinis , Kalnenieks Uldis
Published: November 2023

Understanding the energy metabolism and its regulation is one of the clues to metabolic engineering of stress-resistant lignocellulose-converting microbial strains, also including the promising ethanologen Zymomonas mobilis. Z. mobilis is an obligately fermentative, facultatively anaerobic bacterium, carrying an active respiratory chain with low energy-coupling efficiency. Its respiration does not supply energy to aerobically growing cultures on sugary media, yet oxidative phosphorylation has been demonstrated in non-growing cells with ethanol. Here, we show, for the first time, that in respiring, non-growing Z. mobilis cells receiving regular small amounts of ethanol, oxidative phosphorylation significantly contributes to the maintenance of their viability. No improvement of viability is seen in the NADH dehydrogenase (ndh)-deficient respiratory mutant, which is unable to oxidize ethanol. The ethanol effect is also hampered by the protonophoric uncoupler CCCP, or the inhibitor of ATP synthase, DCCD. At higher concentrations (6% v/v), ethanol causes stress that slows down culture growth. By monitoring the activity of several respiratory gene promoters under ethanol stress with the green fluorescent protein reporter system, we demonstrate downregulation of these promoters, in particular the ndh promoter. We speculate that the decrease in respiratory chain activity in response to stress conditions mitigates the production of reactive oxygen species.
Dynamics of Active Fluorescent Units (AFU) and Water Activity (aw) Changes in Probiotic Products—Pilot Study
Authors: Sielatycka Katarzyna , Śliwa-Dominiak Joanna , Radaczyńska Martyna , Juzwa Wojciech , Kaczmarczyk Mariusz , Marlicz Wojciech , Skonieczna-Żydecka Karolina , Łoniewski Igor
Published: November 2023

The flow cytometry method (FCM) is a widely renowned practice increasingly used to assess the microbial viability of probiotic products. Additionally, the measurement of water activity (aw) can be used to confirm the presence of viable cells in probiotic products throughout their shelf lives. The aim of this study was to investigate the correlation between changes in aw and variations in active fluorescent units (AFU), a unit commonly used in flow cytometry method, during the aging of probiotic products containing freeze-dried bacteria. We controlled the stability of probiotic products for bacterial counts (using ISO 19344 method) and aw levels in commercially available capsules containing freeze-dried bacteria such as Lactobacillus sp. or combinations of Lactobacillus sp. and Bifidobacterium sp. in standard conditions (25 ± 2 °C and 60% relative humidity) over a period of 24 months. During this time, the bacterial contents decreased by 0.12 Log10 in the single-strain product, by 0.16 Log10 in the two-strain product and by 0.26 Log10 in the multi-strain product. With the increase in aw, the number of bacteria decreased but the aw at the end point of the stability study did not exceed 0.15 in each of the three tested products. FCM combined with aw is a prospective analysis that can be used to assess the stability of probiotic products, both for its ability to detect bacterial viability and for practical (analysis time) and economic reasons.
Carcass Yields and Meat Composition of Roosters of the Portuguese Autochthonous Poultry Breeds: “Branca”, “Amarela”, “Pedrês Portuguesa”, and “Preta Lusitânica”
Authors: Meira Márcio , Afonso Isabel M. , Cruz Rebeca , Lopes Júlio Cesar , Martins Raquel S. , Domingues Jéssica , Ribeiro Virgínia , Dantas Rui , Casal Susana , Brito Nuno V.
Published: November 2023

Poultry meat is an important part of the human diet, and the valorisation of autochthonous breeds is a determinant for the sustainability of the rural areas. The increasing demand for niche products demands for better knowledge of the carcass characteristics and meat quality of these local populations. The present study aims to characterise the roosters’ meat from the “Branca” (BR), “Amarela” (AM), “Pedrês Portuguesa” (PP), and “Preta Lusitânica” (PL) breeds. A total of 80 birds (n = 20 per breed) between 38 and 42 weeks old were slaughtered. The physicochemical and nutritional composition were determined in the breast and drumstick meat. The meat of the PL breed had a higher (p ≤ 0.05) pH value, the AM meat revealed a water-holding capacity (WHC) of superior value and moisture content (p ≤ 0.05), while the BR breed had the highest (p ≤ 0.05) ash content. On the other hand, it was observed that the PP meat had a higher (p ≤ 0.05) yellowness index (b*). The breast meat exhibited, in all breeds, a lower pH value, WHC, redness (a*), and lipid content and greater (p ≤ 0.05) lightness (L*), b*, moisture, and ash and protein contents compared to the drumstick. Furthermore, it presented higher (p ≤ 0.05) K, P, and Mg contents and a superior proportion of total and sensorial relevant amino acids. Regarding the fatty acid profile, the breed differences were more significant in the drumstick, with the AM breed lipids presenting a higher (p ≤ 0.05) percentage of MUFAs in the fat, a lower atherogenic index, and a higher (p ≤ 0.05) value for the hypocholesterolemic/hypercholesterolemic fatty acids ratio, while the BR breed lipids presented a higher (p ≤ 0.05) percentage of PUFAs and PUFAs/SFAs ratio and a lower n-6/n-3 ratio. Considering the results obtained, it can be concluded that the meat from these breeds is a wholesome dietary option, distinguished by a favourable overall nutritional composition marked by elevated protein content, reduced lipid amounts, and amino and fatty acid profiles with desirable nutritional indices.
Design of an Artificial Intelligence of Things-Based Sesame Oil Evaluator for Quality Assessment Using Gas Sensors and Deep Learning Mechanisms
Authors: Ku Hao-Hsiang , Lung Ching-Fu , Chi Ching-Ho
Published: November 2023

Traditional oil quality measurement is mostly based on chemical indicators such as acid value, peroxide value, and p-anisidine value. This process requires specialized knowledge and involves complex steps. Hence, this study designs and proposes a Sesame Oil Quality Assessment Service Platform, which is composed of an Intelligent Sesame Oil Evaluator (ISO Evaluator) and a Cloud Service Platform. Users can quickly assess the quality of sesame oil using this platform. The ISO Evaluator employs Artificial Intelligence of Things (AIoT) sensors to detect changes in volatile gases and the color of the oil during storage. It utilizes deep learning mechanisms, including Artificial Neural Network (ANN), Convolutional Neural Network (CNN), and Long Short-Term Memory (LSTM) to determine and evaluate the quality of the sesame oil. Evaluation results demonstrate that the linear discriminant analysis (LDA) value is 95.13. The MQ2, MQ3, MQ4, MQ7, and MQ8 sensors have a positive correlation. The CNN combined with an ANN model achieves a Mean Absolute Percentage Error (MAPE) of 8.1820% for predicting oil quality, while the LSTM model predicts future variations in oil quality indicators with a MAPE of 0.44%. Finally, the designed Sesame Oil Quality Assessment Service Platform effectively addresses issues related to digitization, quality measurement, supply quality observation, and scalability.
Copper Ion Removal Using a Waste-Plastic-Derived Hydrogel Adsorbent Prepared via Microwave-Assisted PET Aminolysis
Authors: Chan Kayee , Kawai Masami , Yamake Mina , Zinchenko Anatoly
Published: November 2023

Despite the tremendous progress in the development of functional materials from plastic waste to promote its recycling, only a few examples of hydrogel materials from plastic waste were reported. In this study, microwave-assisted depolymerization of waste PET plastic using polyamine was performed to prepare short aminophthalamide oligomers followed by chemically cross-linking into a hydrogel material. Catalyst-free microwave-assisted aminolysis of PET was completed within 30–40 s, demonstrating high efficiency of the depolymerization reaction. Subsequent epoxy cross-linking of the oligomers yielded a hydrogel with a swelling degree of ca. 92.1 times in pure water. The application of the obtained hydrogel for the removal of copper ions (Cu2+) from water was demonstrated. Efficient complexation of NH2 groups of the hydrogel with Cu2+ resulted in high adsorption capacities of the hydrogel material toward Cu2+ removal, which were the highest at neutral pHs and reached ca. 213 mg/g. The proposed type of environmental material is beneficial owing to its waste-derived nature and functionality that can be applied for the high-efficiency removal of a broad scope of known environmental pollutants.
Effectiveness of the Geoeducational Assessment Method (GEOAM) in Unveiling Geoeducational Potential: A Case Study of Samos
Authors: Zafeiropoulos George , Drinia Hara
Published: November 2023

This paper explores the efficacy of the geoeducational assessment method (GEOAM) in evaluating the geoeducational potential of geosites. Leveraging a case study involving four geotopes on the island of Samos, Aegean Sea, Greece, this study examines the strengths and limitations of the GEOAM approach, aiming to comprehensively elucidate its efficacy. The assessment outcomes illuminate the vital role of targeted strategies in enhancing the educational and sustainable impact of geosites, thereby fostering geological understanding and responsible environmental engagement. A prominent finding is the urgency to address the gap in foundational geological knowledge, underscored by the need for robust geoeducation programs at schools and the augmented presence of geologists. While acknowledging potential limitations, including subjectivity in scoring and data availability constraints, this study underscores the method’s broader contribution to societal goals. By integrating geoethic principles, GEOAM offers a comprehensive framework aligning with the objectives of geological comprehension and environmentally conscious practices.
Patients’ Perspective on Termination of Pacemaker Therapy—A Cross-Sectional Anonymous Survey among Patients Carrying a Pacemaker in Germany
Authors: Portig Irene , Karaaslan Elif , Hofacker Elena , Volberg Christian , Seifart Carola
Published: November 2023

Objective: To determine the opinions of patients regarding the withdrawal of pacemaker therapy. Participants and methods: A cross-sectional anonymous questionnaire was administered to patients visiting an outpatient cardiologic clinic for routine follow-up visits of pacemaker therapy or patients carrying a pacemaker admitted to a hospital between 2021 and 2022. Results: Three-hundred and forty patients answered the questionnaire. A total of 56% of the participants were male. The mean age was 81 years. The majority of respondents were very comfortable with their PM and felt well informed, with one exception: more than half of respondents were missing information on withdrawal of pacemaker therapy. Almost two-thirds wanted to decide for themselves if their pacemaker therapy was withdrawn regardless of whether they were ill or healthy. Almost 60% of patients would like the pacemaker to be turned off when dying. Women expressed this wish significantly more often than men. Conclusion: Our survey shows that patients prefer to be informed on issues regarding the withdrawal of pacemakers as early as preimplantation. Also, patients would like to be involved in decisions that have to be made at the end of life, including decisions on withdrawal. Offers of conversations about this important issue should include information on special features of the patient’s pacemaker, e.g., the absence or presence of pacemaker dependency. Knowledge about the pacemaker’s functionality may prevent distress among individuals nearing their end of life when, for example, under the false impression that timely deactivation may allow for a more peaceful death.
Extreme Value Theory Modelling of the Behaviour of Johannesburg Stock Exchange Financial Market Data
Authors: Metwane Maashele Kholofelo , Maposa Daniel
Published: November 2023

Financial market data are abundant with outliers, and the search for an appropriate extreme value theory (EVT) approach to apply is an endless debate in the statistics of extremes research. This paper uses EVT methods to model the five-year daily all-share total return index (ALSTRI) and the daily United States dollar (USD) against the South African rand (ZAR) exchange rate of the Johannesburg stock exchange (JSE). The study compares the block maxima approach and the peaks-over-threshold (POT) approach in terms of their ability to model financial market data. The 100-year return levels for the block maxima approach were found to be almost equal to the maximum observations of the financial markets of 10,860 and R18.99 for the ALSTRI and the USD–ZAR, respectively. For the peaks-over-threshold (POT) approach, the results show that the ALSTRI and the USD–ZAR exchange rate will surpass 17,501.63 and R23.72, respectively, at least once in 100 years. The findings in this study reveal a clear distinction between block maxima and POT return level estimates. The POT approach return level estimates were comparably higher than the block maxima estimates. The study further revealed that the blended generalised extreme value (bGEVD) is more suitable for relatively short-term forecasting, since it cuts off at the 50-year return level. Therefore, this study will add value to the literature and knowledge of statistics and econometrics. In the future, more studies on bGEVD, vine copulas, and the r-largest-order bGEVD can be conducted in the financial markets.
The N-Terminal Part of Drosophila CP190 Is a Platform for Interaction with Multiple Architectural Proteins
Authors: Golovnin Anton , Melnikova Larisa , Babosha Valentin , Pokholkova Galina V. , Slovohotov Ivan , Umnova Anastasia , Maksimenko Oksana , Zhimulev Igor F. , Georgiev Pavel
Published: November 2023

CP190 is a co-factor in many Drosophila architectural proteins, being involved in the formation of active promoters and insulators. CP190 contains the N-terminal BTB/POZ (Broad-Complex, Tramtrack and Bric a brac/POxvirus and Zinc finger) domain and adjacent conserved regions involved in protein interactions. Here, we examined the functional roles of these domains of CP190 in vivo. The best-characterized architectural proteins with insulator functions, Pita, Su(Hw), and dCTCF, interacted predominantly with the BTB domain of CP190. Due to the difficulty of mutating the BTB domain, we obtained a transgenic line expressing a chimeric CP190 with the BTB domain of the human protein Kaiso. Another group of architectural proteins, M1BP, Opbp, and ZIPIC, interacted with one or both of the highly conserved regions in the N-terminal part of CP190. Transgenic lines of D. melanogaster expressing CP190 mutants with a deletion of each of these domains were obtained. The results showed that these mutant proteins only partially compensated for the functions of CP190, weakly binding to selective chromatin sites. Further analysis confirmed the essential role of these domains in recruitment to regulatory regions associated with architectural proteins. We also found that the N-terminal of CP190 was sufficient for recruiting Z4 and Chromator proteins and successfully achieving chromatin opening. Taken together, our results and the results of previous studies showed that the N-terminal region of CP190 is a platform for simultaneous interaction with various DNA-binding architectural proteins and transcription complexes.
A Single Injection of rAAV-shmTOR in Peripheral Nerve Persistently Attenuates Nerve Injury-Induced Mechanical Allodynia
Authors: Park Minkyung , Woo Ha-Na , Koh Chin Su , Chang Heesue , Kim Ji Hyun , Park Keerang , Chang Jin Woo , Lee Heuiran , Jung Hyun Ho
Published: November 2023

Activation of mammalian target of rapamycin (mTOR) has been known as one of the contributing factors in nociceptive sensitization after peripheral injury. Its activation followed by the phosphorylation of downstream effectors causes hyperexcitability of primary sensory neurons in the dorsal root ganglion. We investigated whether a single injection of rAAV-shmTOR would effectively downregulate both complexes of mTOR in the long-term and glial activation as well. Male SD rats were categorized into shmTOR (n = 29), shCON (n = 23), SNI (n = 13), and Normal (n = 8) groups. Treatment groups were injected with rAAV-shmTOR or rAAV-shCON, respectively. DRG tissues and sciatic nerve were harvested for Western blot and immunohistochemical analyses. Peripheral sensitization was gradually attenuated in the shmTOR group, and it reached a peak on PID 21. Western blot analysis showed that both p-mTORC1 and p-mTORC2 were downregulated in the DRG compared to shCON and SNI groups. We also found decreased expression of phosphorylated p38 and microglial activation in the DRG. We first attempted a therapeutic strategy for neuropathic pain with a low dose of AAV injection by interfering with the mTOR signaling pathway, suggesting its potential application in pain treatment.
Artificial Light for Improving Tomato Recovery Following Grafting: Transcriptome and Physiological Analyses
Authors: Ding Xiaotao , Miao Chen , Li Rongguang , He Lizhong , Zhang Hongmei , Jin Haijun , Cui Jiawei , Wang Hong , Zhang Yongxue , Lu Panling , Zou Jun , Yu Jizhu , Jiang Yuping , Zhou Qiang
Published: November 2023

Grafting is widely used to enhance the phenotypic traits of tomatoes, alleviate biotic and abiotic stresses, and control soil-borne diseases of the scion in greenhouse production. There are many factors that affect the healing and acclimatization stages of seedlings after grafting. However, the role of light has rarely been studied. In this study, we compared the effects of artificial light and traditional shading (under shaded plastic-covered tunnels) on the recovery of grafted tomato seedlings. The results show that the grafted tomato seedlings recovered using artificial light had a higher healthy index, leaf chlorophyll content, shoot dry weight, and net photosynthetic rate (Pn) and water use efficiency (WUE) compared with grafted seedling recovered using the traditional shading method. Transcriptome analysis showed that the differentially expressed genes (DEGs) of grafted seedlings restored using artificial light were mainly enriched in the pathways corresponding to plant hormone signal transduction. In addition, we measured the endogenous hormone content of grafted tomato seedlings. The results show that the contents of salicylic acid (SA) and kinetin (Kin) were significantly increased, and the contents of indoleacetic acid (IAA) and jasmonic acid (JA) were decreased in artificial-light-restored grafted tomato seedlings compared with those under shading treatments. Therefore, we suggest that artificial light affects the morphogenesis and photosynthetic efficiency of grafted tomato seedlings, and it can improve the performance of tomato seedlings during grafting recovery by regulating endogenous hormone levels.
Improving the Efficacy of Magnetic Nanoparticle-Mediated Hyperthermia Using Trapezoidal Pulsed Electromagnetic Fields as an In Vitro Anticancer Treatment in Melanoma and Glioblastoma Multiforme Cell Lines
Authors: Souiade Lilia , Domingo-Diez Javier , Alcaide Cesar , Gámez Berta , Gámez Linarejos , Ramos Milagros , Serrano Olmedo José Javier
Published: November 2023

Magnetic hyperthermia (MHT) is an oncological therapy that uses magnetic nanoparticles (MNPs) to generate localized heat under a low-frequency alternating magnetic field (AMF). Recently, trapezoidal pulsed alternating magnetic fields (TPAMFs) have proven their efficacy in enhancing the efficiency of heating in MHT as compared to the sinusoidal one. Our study aims to compare the TPAMF waveform’s killing effect against the sinusoidal waveform in B16F10 and CT2A cell lines to determine more efficient waveforms in causing cell death. For that purpose, we used MNPs and different AMF waveforms: trapezoidal (TP), almost-square (TS), triangular (TR), and sinusoidal signal (SN). MNPs at 1 and 4 mg/mL did not affect cell viability during treatment. The exposition of B16F10 and CT2A cells to only AMF showed nonsignificant mortality. Hence, the synergetic effect of the AMF and MNPs causes the observed cell death. Among the explored cases, the nonharmonic signals demonstrated better efficacy than the SN one as an MHT treatment. This study has revealed that the application of TP, TS, or TR waveforms is more efficient and has considerable capability to increase cancer cell death compared to the traditional sinusoidal treatment. Overall, we can conclude that the application of nonharmonic signals enhances MHT treatment efficiency against tumor cells.
Expression, Characterisation, Homology Modelling and Molecular Docking of a Novel M17 Family Leucyl-Aminopeptidase from Bacillus cereus CZ
Authors: Liu Jie , Cui Tangbing
Published: November 2023

Leucyl-aminopeptidase (LAP), an important metallopeptidase, hydrolyses amino acid residues from the N-terminus of polypeptides and proteins, acting preferentially on the peptide bond formed by N-terminus leucine. A new leucyl-aminopeptidase was found in Bacillus cereus CZ. Its gene (bclap) contained a 1485 bp ORF encoding 494 amino acids with a molecular weight of 54 kDa. The bcLAP protein was successfully expressed in E. coli BL21(DE3). Optimal activity is obtained at pH 9.0 and 58 °C. The bcLAP displays a moderate thermostability and an alkaline pH adaptation range. Enzymatic activity is dramatically enhanced by Ni2+. EDTA significantly inhibits the enzymatic activity, and bestatin and SDS also show strong inhibition. The three-dimensional model of bcLAP monomer and homohexamer is simulated byPHYRE2 server and SWISS-MODEL server. The docking of bestatin, Leu-Trp, Asp-Trp and Ala-Ala-Gly to bcLAP is performed using AutoDock4.2.5, respectively. Molecular docking results show that the residues Lys260, Asp265, Lys272, Asp283, Asp342, Glu344, Arg346, Gly372 and His437 are involved in the hydrogen bonding with the ligands and zinc ions. There may be two nucleophilic catalytic mechanisms in bcLAP, one involving His 437 or Arg346 and the other involving His437 and Arg346. The bcLAP can hydrolyse the peptide bonds in Leu-Trp, Asp-Trp and Ala-Ala-Gly.
Expression of Cannabinoid Receptors in the Trigeminal Ganglion of the Horse
Authors: Zamith Cunha Rodrigo , Semprini Alberto , Salamanca Giulia , Gobbo Francesca , Morini Maria , Pickles Kirstie J. , Roberts Veronica , Chiocchetti Roberto
Published: November 2023

Cannabinoid receptors are expressed in human and animal trigeminal sensory neurons; however, the expression in the equine trigeminal ganglion is unknown. Ten trigeminal ganglia from five horses were collected post-mortem from an abattoir. The expression of cannabinoid receptors type 1 (CB1R) and type 2 (CB2R), and the cannabinoid-related receptors like transient receptor potential vanilloid type 1 (TRPV1), peroxisome proliferator-activated receptor gamma (PPARɣ), and G protein-related receptor 55 (GPR55) in the trigeminal ganglia (TG) of the horse were studied, using immunofluorescence on cryosections and formalin-fixed paraffin-embedded (FFPE) sections. Neurons and glial cells were identified using fluorescent Nissl staining NeuroTrace® and an antibody directed against the glial marker glial fibrillary acidic protein (GFAP), respectively. Macrophages were identified by means of an antibody directed against the macrophages/microglia marker ionized calcium-binding adapter molecule 1 (IBA1). The protein expression of CB1R, CB2R, TRPV1, and PPARɣ was found in the majority of TG neurons in both cryosections and FFPE sections. The expression of GPR55 immunoreactivity was mainly detectable in FFPE sections, with expression in the majority of sensory neurons. Some receptors were also observed in glial cells (CB2R, TRPV1, PPARγ, and GPR55) and inflammatory cells (PPARγ and GPR55). These results support further investigation of such receptors in disorders of equine trigeminal neuronal excitability.
Mitochondrial Complex I and β-Amyloid Peptide Interplay in Alzheimer’s Disease: A Critical Review of New and Old Little Regarded Findings
Authors: Atlante Anna , Valenti Daniela
Published: November 2023

Alzheimer’s disease (AD) is the most common neurodegenerative disorder and the main cause of dementia which is characterized by a progressive cognitive decline that severely interferes with daily activities of personal life. At a pathological level, it is characterized by the accumulation of abnormal protein structures in the brain—β-amyloid (Aβ) plaques and Tau tangles—which interfere with communication between neurons and lead to their dysfunction and death. In recent years, research on AD has highlighted the critical involvement of mitochondria—the primary energy suppliers for our cells—in the onset and progression of the disease, since mitochondrial bioenergetic deficits precede the beginning of the disease and mitochondria are very sensitive to Aβ toxicity. On the other hand, if it is true that the accumulation of Aβ in the mitochondria leads to mitochondrial malfunctions, it is otherwise proven that mitochondrial dysfunction, through the generation of reactive oxygen species, causes an increase in Aβ production, by initiating a vicious cycle: there is therefore a bidirectional relationship between Aβ aggregation and mitochondrial dysfunction. Here, we focus on the latest news—but also on neglected evidence from the past—concerning the interplay between dysfunctional mitochondrial complex I, oxidative stress, and Aβ, in order to understand how their interplay is implicated in the pathogenesis of the disease.
Mrp and SufT, Two Bacterial Homologs of Eukaryotic CIA Factors Involved in Fe-S Clusters Biogenesis
Authors: Aubert Corinne , Mandin Pierre , Py Béatrice
Published: November 2023

Fe-S clusters are essential cofactors for the activity of a large variety of metalloproteins that play important roles in respiration, photosynthesis, nitrogen fixation, regulation of gene expression, and numerous metabolic pathways, including biosynthesis of other protein cofactors. Assembly of iron and sulfur atoms into a cluster, followed by its insertion into the polypeptide chain, is a complex process ensured by multiproteic systems. Through evolution, eukaryotes have acquired two Fe-S protein biogenesis systems by endosymbiosis from bacteria. These systems, ISC and SUF, are compartmentalized in mitochondria and plastids, respectively. The eukaryotic Fe-S protein biogenesis system (CIA) is dedicated to the biogenesis of cytosolic and nuclear Fe-S proteins. While the CIA system is absent in bacteria, at least two of its components share homologies with bacterial Fe-S protein biogenesis factors, Mrp and SufT. Here, we provide an overview of the role of Mrp and SufT in Fe-S protein biogenesis in bacteria, aiming to put forward specific but also common features with their eukaryotic CIA counterparts.
Extruder Machine Gear Fault Detection Using Autoencoder LSTM via Sensor Fusion Approach
Authors: Lee Joon-Hyuk , Okwuosa Chibuzo Nwabufo , Hur Jang-Wook
Published: November 2023

In industrial settings, gears play a crucial role by assisting various machinery functions such as speed control, torque manipulation, and altering motion direction. The malfunction or failure of these gear components can have serious repercussions, resulting in production halts and financial losses. To address this need, research efforts have focused on early defect detection in gears in order to reduce the impact of possible failures. This study focused on analyzing vibration and thermal datasets from two extruder machine gearboxes using an autoencoder Long Short-Term Memory (AE-LSTM) model, to ensure that all important characteristics of the system are utilized. Fast independent component analysis (FastICA) is employed to fuse the data signals from both sensors while retaining their characteristics. The major goal is to implement an outlier detection approach to detect and classify defects. The results of this study highlighted the extraordinary performance of the AE-LSTM model, which achieved an impressive accuracy rate of 94.42% in recognizing malfunctioning gearboxes within the extruder machine system. The study used robust global metric evaluation techniques, such as accuracy, F1-score, and confusion metrics, to thoroughly evaluate the model’s dependability and efficiency. LSTM was additionally employed for anomaly detection to further emphasize the adaptability and interoperability of the methodology. This modification yielded a remarkable accuracy of 89.67%, offering additional validation of the model’s reliability and competence.
Evaluation of Humoral Response following SARS-CoV-2 mRNA-Based Vaccination in Liver Transplant Recipients Receiving Tailored Immunosuppressive Therapy
Authors: Manzia Tommaso Maria , Sensi Bruno , Conte Luigi Eduardo , Siragusa Leandro , Angelico Roberta , Frongillo Francesco , Tisone Giuseppe
Published: November 2023

Background: The role of tailored immunosuppression (IS) in the development of the humoral response (HR) to SARS-CoV-2 mRNA-based vaccination in liver transplant (LT) recipients is unknown. Methods: This is a single-centre prospective study of patients who underwent LT between January 2015 and December 2021 and who have received three doses of mRNA-based SARS-CoV-2 vaccination. Patients undergoing Tacrolimus-based immunosuppression (TAC-IS) were compared with those undergoing Everolimus-based immunosuppression (EVR-IS). Patients receiving the TAC-EVR combination were divided into two groups based on trough TAC concentrations, i.e., above or below 5 ng/mL. HR (analysed with ECLIA) was assessed at 30 to 135 days after vaccination. The primary outcome was the presence of a positive antibody titre (≥0.8 U/mL). Secondary outcomes were the presence of a highly protective antibody titre (≥142 U/mL), median antibody titre, and incidence of COVID-19. Results: Sixty-one participants were included. Twenty-four (40%) were receiving TAC-IS and thirty-seven (60%) were receiving EVR-IS. At the median follow-up of 116 (range: 89–154) days, there were no significant differences in positive antibody titre (95.8% vs. 94.6%; p = 0.8269), highly-protective antibody titre (83.3% vs. 81.1%; p = 0.8231), median antibody titre (2410 [IQ range 350–2500] vs. 1670 [IQ range 380–2500]; p = 0.9450), and COVID-19 incidence (0% vs. 5.4%; p = 0.5148). High serum creatinine and low estimated glomerular filtration rate were risk factors for a weak or absent HR. Conclusions: Three doses of mRNA-based SARS-CoV-2 vaccination yielded a highly protective HR in LT recipients. The use of TAC or EVR-based IS does not appear to influence HR or antibody titre, while renal disease is a risk factor for a weak or null HR.
Assessing Procedural Accuracy in Lateral Spine Surgery: A Retrospective Analysis of Percutaneous Pedicle Screw Placement with Intraoperative CT Navigation
Authors: Hiyama Akihiko , Sakai Daisuke , Katoh Hiroyuki , Nomura Satoshi , Watanabe Masahiko
Published: November 2023

Percutaneous pedicle screws (PPSs) are commonly used in posterior spinal fusion to treat spine conditions such as trauma, tumors, and degenerative diseases. Precise PPS placement is essential in preventing neurological complications and improving patient outcomes. Recent studies have suggested that intraoperative computed tomography (CT) navigation can reduce the dependence on extensive surgical expertise for achieving accurate PPS placement. However, more comprehensive documentation is needed regarding the procedural accuracy of lateral spine surgery (LSS). In this retrospective study, we investigated patients who underwent posterior instrumentation with PPSs in the thoracic to lumbar spine, utilizing an intraoperative CT navigation system, between April 2019 and September 2023. The system’s methodology involved real-time CT-based guidance during PPS placement, ensuring precision. Our study included 170 patients (151 undergoing LLIF procedures and 19 trauma patients), resulting in 836 PPS placements. The overall PPS deviation rate, assessed using the Ravi scale, was 2.5%, with a notably higher incidence of deviations observed in the thoracic spine (7.4%) compared to the lumbar spine (1.9%). Interestingly, we found no statistically significant difference in screw deviation rates between upside and downside PPS placements. Regarding perioperative complications, three patients experienced issues related to intraoperative CT navigation. The observed higher rate of inaccuracies in the thoracic spine suggests that various factors may contribute to these differences in accuracy, including screw size and anatomical variations. Further research is required to refine PPS insertion techniques, particularly in the context of LSS. In conclusion, this retrospective study sheds light on the challenges associated with achieving precise PPS placement in the lateral decubitus position, with a significantly higher deviation rate observed in the thoracic spine compared to the lumbar spine. This study emphasizes the need for ongoing research to improve PPS insertion techniques, leading to enhanced patient outcomes in spine surgery.
The Role of Chest Compressions on Ventilation during Advanced Cardiopulmonary Resuscitation
Authors: Azcarate Izaskun , Urigüen Jose Antonio , Leturiondo Mikel , Sandoval Camilo Leonardo , Redondo Koldo , Gutiérrez José Julio , Russell James Knox , Wallmüller Pia , Sterz Fritz , Daya Mohamud Ramzan , Ruiz de Gauna Sofía
Published: November 2023

Background: There is growing interest in the quality of manual ventilation during cardiopulmonary resuscitation (CPR), but accurate assessment of ventilation parameters remains a challenge. Waveform capnography is currently the reference for monitoring ventilation rate in intubated patients, but fails to provide information on tidal volumes and inspiration–expiration timing. Moreover, the capnogram is often distorted when chest compressions (CCs) are performed during ventilation compromising its reliability during CPR. Our main purpose was to characterize manual ventilation during CPR and to assess how CCs may impact on ventilation quality. Methods: Retrospective analysis were performed of CPR recordings fromtwo databases of adult patients in cardiac arrest including capnogram, compression depth, and airway flow, pressure and volume signals. Using automated signal processing techniques followed by manual revision, individual ventilations were identified and ventilation parameters were measured. Oscillations on the capnogram plateau during CCs were characterized, and its correlation with compression depth and airway volume was assessed. Finally, we identified events of reversed airflow caused by CCs and their effect on volume and capnogram waveform. Results: Ventilation rates were higher than the recommended 10 breaths/min in 66.7% of the cases. Variability in ventilation rates correlated with the variability in tidal volumes and other ventilatory parameters. Oscillations caused by CCs on capnograms were of high amplitude (median above 74%) and were associated with low pseudo-volumes (median 26 mL). Correlation between the amplitude of those oscillations with either the CCs depth or the generated passive volumes was low, with correlation coefficients of −0.24 and 0.40, respectively. During inspiration and expiration, reversed airflow events caused opposed movement of gases in 80% of ventilations. Conclusions: Our study confirmed lack of adherence between measured ventilation rates and the guideline recommendations, and a substantial dispersion in manual ventilation parameters during CPR. Oscillations on the capnogram plateau caused by CCs did not correlate with compression depth or associated small tidal volumes. CCs caused reversed flow during inspiration, expiration and in the interval between ventilations, sufficient to generate volume changes and causing oscillations on capnogram. Further research is warranted to assess the impact of these findings on ventilation quality during CPR.
The Potential Benefic Effect of Nicotinamide Riboside in Treating a Murine Model of Monoiodoacetate-Induced Knee Osteoarthritis
Authors: Gherghina Florin-Liviu , Mîndrilă Ion , Buteică Sandra-Alice , Bică George , Pisoschi Cătălina-Gabriela , Biță Cristina-Elena , Paliu Iulia-Alexandra , Rogoveanu Otilia-Constantina
Published: November 2023

Knee osteoarthritis (KOA), one of the most common orthopedic disorders concerning the adult population worldwide, is a condition characterized by progressive destruction of the articular cartilage and the presence of an inflammatory process. The aim of our study was to assess whether nicotinamide riboside (NR), a popular anti-aging supplement, can reduce the rate of cartilage destruction and alleviate the inflammatory response compared to the commonly prescribed collagen supplement in a murine monoiodoacetate (MIA)-induced KOA model. Twenty Wistar rats were randomly assigned to 4 groups: sham (S), MIA and NR, MIA and hydrolyzed collagen (HC), and MIA. At the end of the experiment, the right knees and blood samples were collected for histological assessment and biochemical evaluation of nitric oxide, malondialdehyde, total antioxidant capacity, reduced glutathione, glutathione peroxidase, superoxide dismutase, catalase, myeloperoxidase, and tumoral necrosis factor-alpha (TNF-α). The study determined that the treatment with NR in a similar dose with HC decreased blood/serum levels of oxidative stress biomarkers and the histological lesions in almost the same manner. The present findings suggest that NR may exhibit chondroprotective and anti-inflammatory effects in MIA-induced KOA in rats.
Autoimmune Heparin-Induced Thrombocytopenia
Authors: Warkentin Theodore E.
Published: November 2023

Autoimmune thrombocytopenia (aHIT) is a severe subtype of heparin-induced thrombocytopenia (HIT) with atypical clinical features caused by highly pathological IgG antibodies (“aHIT antibodies”) that activate platelets even in the absence of heparin. The clinical features of aHIT include: the onset or worsening of thrombocytopenia despite stopping heparin (“delayed-onset HIT”), thrombocytopenia persistence despite stopping heparin (“persisting” or “refractory HIT”), or triggered by small amounts of heparin (heparin “flush” HIT), most cases of fondaparinux-induced HIT, and patients with unusually severe HIT (e.g., multi-site or microvascular thrombosis, overt disseminated intravascular coagulation [DIC]). Special treatment approaches are required. For example, unlike classic HIT, heparin cessation does not result in de-escalation of antibody-induced hemostasis activation, and thus high-dose intravenous immunoglobulin (IVIG) may be indicated to interrupt aHIT-induced platelet activation; therapeutic plasma exchange may be required if high-dose IVIG is ineffective. Also, aHIT patients are at risk for treatment failure with (activated partial thromboplastin time [APTT]-adjusted) direct thrombin inhibitor (DTI) therapy (argatroban, bivalirudin), either because of APTT confounding (where aHIT-associated DIC and resulting APTT prolongation lead to systematic underdosing/interruption of DTI therapy) or because DTI inhibits thrombin-induced protein C activation. Most HIT laboratories do not test for aHIT antibodies, contributing to aHIT under-recognition.
Outcomes of Meniscal Repair in Patients Aged 40 and Above: A Systematic Review
Authors: Jaibaji Rawan , Khaleel Faisal , Jaibaji Monketh , Volpin Andrea
Published: November 2023

Purpose: Meniscal injuries are increasingly common in older age groups. Age is often cited as a contraindication to undergoing meniscal repair due to concerns regarding failure rates. There has recently, however, been an increasing shift towards repair in older populations. The purpose of this study was to review outcomes of meniscal repair in patients over the age of 40. Methods: A systematic search of the following databases was conducted of PubMed, SCOPUS, Web of Science, and Cochrane Library to identify studies reporting failure rates of patients over 40 with meniscal injuries undergoing repair. The definition of meniscus failure was noted for each study evaluated in this systematic review. Further data surrounding clinical and radiological outcomes were recorded and evaluated, when available. Results: Thirteen studies were included in this review, encompassing a total of 316 meniscal repairs in patients over the age of 40 years. The overall failure rate was found to be 15.5% (49/316) (range 0–33.3%). There was no difference in the failure rate in those over 40 vs. under 40, and the two groups had equivalent functional outcomes. Conclusions: Age should not be considered a contra-indication for meniscal repair. Appropriately selected older patients can have acceptably low failure rates with meniscal repair and similar functional outcomes to those under the age of forty. Meniscal repairs in those over 40 achieved better functional outcomes than patients of the same age group who underwent meniscectomy.