bluuebunny/crossref_metadata_2025 huggingface dataset:
Inferring Dealer Networks in the Foreign Exchange Market Using Conditional Transfer Entropy: Analysis of a Central Bank Announcement
Authors: Janczewski Aleksander , Anagnostou Ioannis , Kandhai Drona
Published: August 2024

The foreign exchange (FX) market has evolved into a complex system where locally generated information percolates through the dealer network via high-frequency interactions. Information related to major events, such as economic announcements, spreads rapidly through this network, potentially inducing volatility, liquidity disruptions, and contagion effects across financial markets. Yet, research on the mechanics of information flows in the FX market is limited. In this paper, we introduce a novel approach employing conditional transfer entropy to construct networks of information flows. Leveraging a unique, high-resolution dataset of bid and ask prices, we investigate the impact of an announcement by the European Central Bank on the information transfer within the market. During the announcement, we identify key dealers as information sources, conduits, and sinks, and, through comparison to a baseline, uncover shifts in the network topology.
Novel Spaces as Catalysts for Change: Transformative Learning through Transnational Projects
Authors: Caldwell Helen , Whewell Emma , West Amy , Tiplady Helen
Published: August 2024

This paper discusses data related to the final phase of a six-year project entitled Digital Learning Across Boundaries (DLAB): Developing Changemakers. It explores university students’ growing perceptions of themselves as changemakers as they experience novel physical and digital spaces, drawing upon on the reflections of seven undergraduate education students from England who joined students from five European countries in the final year of the project. Using a critical ethnography methodology, this paper embraces reflexivity and immersion in the participants’ experiences. Verbal and written reflections were collated and analysed through inductive coding. The results suggest that the multidimensional and multidisciplinary elements of the project led to transformative learning. Transformative change was experienced as students encountered a series of novel spaces and resolved disorientations that arose within them through collective interaction and personal reflection. This paper concludes that educators value the intersection of learning experiences when planning transnational projects and consider the role of cumulative disorientations, multifaceted novel spaces, and critical discourse in catalysing personal growth.
Introducing a Dilute Single Bath for the Electrodeposition of Cu2(ZnSn)(S)4 for Smooth Layers
Authors: Saeed Mahfouz , González Peña Omar I.
Published: August 2024

Cu2(ZnSn)(S)4 (copper, zinc, tin, and sulfide (CZTS)) provides possible advantages over CuInGaSe2 for thin-film photovoltaic devices because it has a higher band gap. Preparing CZTS by electrodeposition because of its high productivity and lower processing costs, electroplating is appealing. Recently published studies reported that the electrodeposition process of CZTS still faces significant obstacles, such as the sulfur atomic ratio (about half of the whole alloy), deposits’ adhesion, film quality, and optical properties. This work introduces an improved bath that facilitates the direct electroplating of CZTS from one processing step. The precursors used were significantly more diluted than the typical baths mentioned in the last few years. An extensive analysis of the electrochemical behavior at various rotation speeds is presented at room temperature (~22 °C). The deposited alloy’s composition and adherence to the molybdenum back contact are examined with agitation. The annealing process was carried out in an environment containing sulfur, and the metal was not added at this stage. The ultimate sulfur composition was adjusted to 50.2%, about the desired atomic ratio. The compound’s final composition was investigated using the Energy-Dispersive X-ray Spectroscopy technique. Finally, X-ray diffraction analysis was applied to analyze CZTS crystallography and to measure thickness.
Building Facade-Completion Network Based on Dynamic Convolutional GAN
Authors: Cai Zhenhuang , Lin Yangbin , Huang Xingwang , Zhang Zongliang , Wang Zongyue
Published: August 2024

Building facade completion is an important part of digitizing the structures of buildings using computer technology. Due to the intricate textures and structures in building facade images, existing image-completion algorithms cannot accurately restore the rich texture and detailed information. In response, this paper proposes a novel network to simultaneously recover the texture and semantic structural features of building facades. By incorporating dynamic convolutions into each layer of the feature encoder, the shallow layers of the completion network can create a global receptive field, thus enhancing the model’s feature-extraction capability. Additionally, a spatial attention branch is integrated into the dynamic convolution module to boost the correlation between the completion area and its surrounding edge area, resulting in improved edge clarity and accuracy of the completed facade image. Experimental results on multiple public image datasets demonstrate that the proposed model in this paper achieves state-of-the-art results when applied to real-world datasets.
Temporal Feature Prediction in Audio–Visual Deepfake Detection
Authors: Gao Yuan , Wang Xuelong , Zhang Yu , Zeng Ping , Ma Yingjie
Published: August 2024

The rapid growth of deepfake technology, generating realistic manipulated media, poses a significant threat due to potential misuse. Therefore, effective detection methods are urgently needed to prevent malicious use, as current approaches often focus on single modalities or the simple fusion of audio–visual signals, limiting their accuracy. To solve this problem, we propose a deepfake detection scheme based on bimodal temporal feature prediction, which innovatively introduces the idea of temporal feature prediction into the audio–video bimodal deepfake detection task, aiming at fully exploiting the temporal laws of audio–visual modalities. First, pairs of adjacent audio–video sequence clips are used to construct input quadruples, and a dual-stream network is employed to extract temporal feature representations from video and audio, respectively. A video prediction module and an audio prediction module are designed to capture the temporal inconsistencies within each single modality by predicting future temporal features and comparing them with reference features. Then, a projection layer network is designed to align the audio–visual features, using contrastive loss functions to perform contrastive learning and maximize the differences between real and fake video modalities. Experiments on the FakeAVCeleb dataset demonstrate superior performance with an accuracy of 84.33% and an AUC of 89.91%, outperforming existing methods and confirming the effectiveness of our approach in deepfake detection.
Strategic Public Relations Policy for Accelerating Hydrogen Acceptance: Insights from an Expert Survey in South Korea
Authors: Cho Minhyuk , Lee Youhyun , Kim Youngjin , Lee Min Chul
Published: August 2024

Hydrogen has great growth potential due to its green, carbon-neutral nature, but public acceptance is low due to negative perceptions of the dangers associated with hydrogen energy. Safety concerns, particularly related to its flammability and explosiveness, are an obstacle to hydrogen energy policy. In South Korea, recent hydrogen-related explosions have exacerbated these concerns, undermining public confidence. This study developed public relations (PR) strategies to manage risk perception and promote hydrogen energy acceptance by analyzing the opinions of government officials and experts using SWOT factors, the TOWS matrix, and the analytic hierarchy process. The findings highlight the importance of addressing weaknesses and threats in PR efforts. Key weaknesses include Korea’s technological lag and the low localization of core hydrogen technologies, both of which hinder competitiveness and negatively impact public perception of hydrogen energy. Notable threats include deteriorating energy dependency and expanding global carbon regulations. This information can be used to influence attitudes and foster public acceptance of hydrogen energy policies. Emphasizing weaknesses and threats may result in more effective PR strategies, even if they do not directly address the primary concerns of scientific experts. The persuasive insights identified in this study can support future policy communication and PR strategies.
Analysis of the Parameters of an Ecological Power Supply Wire System for Moving and Stabilising the Position of a Floating Dock
Authors: Szelangiewicz Tadeusz , Żelazny Katarzyna , Sobków Szymon
Published: August 2024

This research study aims to develop a system which ensures the mobility of a floating dock and its stability in position during docking operations. The dock is designed for operation in a river canal. In order to dock a ship, it is moved away from the quay over a dock trench. Initial requirements and design criteria for the system were determined. The most important of them include docking in the maximum weather conditions, corresponding to a wind speed of 5° Beaufort (5°B), and a zero-emission target for the power supply system (use of a renewable energy source). A wire and winch system was designed to move the dock and stabilise it in position during docking operations. The system comprises mooring wires which are tied to bollards on both quays, and wire winches mounted on both sides of the dock. The wire winches are hydraulically driven, and the hydraulic pumps, run by electric motors, are powered with batteries charged using photovoltaic panels. Statistical environmental parameters (wind, river current) were analysed and the probability of certain mooring wire loads and the corresponding wire winch power output were quantified. Based on these calculations, the power of photovoltaic panels and capacity of the batteries required to power the dock moving system were determined. This paper discusses the system design as well as the results of trials.
Convolutional Neural Networks Based on Resonance Demodulation of Vibration Signal for Rolling Bearing Fault Diagnosis in Permanent Magnet Synchronous Motors
Authors: Ding Li , Guo Haotian , Bian Liqiang
Published: August 2024

Permanent magnet synchronous motors (PMSMs) are widely used due to their unique advantages. Their transmission system mainly relies on rolling bearings; therefore, monitoring the motor’s working status and fault diagnosis for the rolling bearings are the key focuses. Traditional resonance demodulation methods analyze the vibration signals of bearings to achieve bearing fault diagnosis, but the limiting condition is that the inherent frequency needs to be known. Based on the resonance demodulation method, deep learning methods, such as the convolutional neural network (CNN) model designed in this article, have improved the practicality and effectiveness of diagnosis. A physical explanation of the deep learning model for bearing fault diagnosis is presented in this article, the relationship between resonance demodulation and the 1D CNN is analyzed, and the model is trained and validated. The experimental results show that the CNN model can identify different types of bearing faults. The analysis results of the trained CNN model and the intermediate results indicate that the CNN model is consistent with the resonance demodulation method. The optimized method is verified, proving that the model can achieve the classification and diagnosis of fault bearing data collected under different environments after the optimized training method is adopted.
The Role of the Soil Seed Bank in the Recovery and Restoration of a Burned Amazonian Terra Firme Forest
Authors: Oliveira Vynicius B. , Jardim Mário A. G. , Barros Maria Fabíola , Silva Danilo S. , Vieira Ima C. G. , Tabarelli Marcelo
Published: August 2024

Here, we examine the effects of wildfires on the soil seed bank of a terra firme forest in the eastern Amazon. This seed bank is described via community-level attributes across forest stands exposed to wildfires once or twice, as well as across unburned, old-growth forest stands. A total of 2345 seeds germinated (837.5 seeds/m2). Across all three forest habitats, the soil seed bank was dominated by a small set of light-demanding species, with two to three species accounting for over 80% of all seeds. On the other hand, the seed bank of all habitats completely lacked seeds from the old-growth flora. Wildfires posed no effects relative to seed density and species richness. However, fire (1) reduced beta diversity, (2) caused an 8% increase in herb abundance and a 4% increase in the number of seeds produced by short-lived pioneers, and (3) resulted in a slight impact on taxonomic species composition. Our results suggest that the soil seed bank, while exhibiting high seed densities, is naturally species poor and, thus, relatively resistant to the first fire events. This implies that the recovery of fire-degraded forests will rely on vertebrate-dispersed seeds coming from any remaining well-preserved old-growth forest stands that are present in the landscape and are highly vulnerable to fire.
Effects of Forest Conversion on the Stocks and Stoichiometry of Soil Carbon, Nitrogen, and Phosphorus at a County Scale in Subtropical China
Authors: Ye Hongmeng , Hu Yeqin , Zhu Dehuang , Zheng Shengmeng , Tang Xin , Wu Jintao , Guo Shulin
Published: August 2024

The decline in primary natural forests worldwide has intensified research on the effects of forest transformation on soil carbon (C), nitrogen (N), and phosphorus (P) cycles and stocks. However, the extent to which soil C, N, and P stocks and stoichiometry are affected by forest conversion remains unclear. Here, we examined the effects of forest transformation on soil nutrient storage capacity and stoichiometric characteristics in native broadleaf forests (BFs), plantation forests (PFs), tea gardens (TGs), cultivated lands (CLs), and urban artificial green spaces (GSs) at a county scale in subtropical China. The results showed that the other forest types exhibited significantly reduced soil C and N contents and stocks but increased soil P content and stock compared to BFs. The soil C:N:P stoichiometric ratios for BFs and the converted PFs, TGs, GSs, and CLs were sequentially decreased as follows: 444.8:24.2:1, 95.0:10.0:1, 30.2:3.9:1, 23.1:3.7:1, and 19.4:1.9:1, respectively. Within the altitude (AL) span of 180 to 1200 m surveyed, the AL decided the type of forest conversion and significantly influenced the stock levels and stoichiometric ratios of soil C, N, and P. The results of this study highlight the importance of the ecological management of TGs and the optimization of soil P production in CLs, TGs, and GSs.
Forest Wildfire Risk Assessment of Anning River Valley in Sichuan Province Based on Driving Factors with Multi-Source Data
Authors: Ji Cuicui , Yang Hengcong , Li Xiaosong , Pei Xiangjun , Li Min , Yuan Hao , Cao Yiming , Chen Boyu , Qu Shiqian , Zhang Na , Chun Li , Shi Lingyi , Sun Fuyang
Published: August 2024

Forest fires can lead to a decline in ecosystem functions, such as biodiversity, soil quality, and carbon cycling, causing economic losses and health threats to human societies. Therefore, it is imperative to map forest-fire risk to mitigate the likelihood of forest-fire occurrence. In this study, we utilized the hierarchical analysis process (AHP), a comprehensive weighting method (CWM), and random forest to map the forest-fire risk in the Anning River Valley of Sichuan Province. We selected non-photosynthetic vegetation (NPV), photosynthetic vegetation (PV), normalized difference vegetation index (NDVI), plant species, land use, soil type, temperature, humidity, rainfall, wind speed, elevation, slope, aspect, distance to road, and distance to residential as forest-fire predisposing factors. We derived the following conclusions. (1) Overlaying historical fire points with mapped forest-fire risk revealed an accuracy that exceeded 86%, indicating the reliability of the results. (2) Forest fires in the Anning River Valley primarily occur in February, March, and April, typically months characterized by very low rainfall and dry conditions. (3) Areas with high and medium forest-fire risk were mainly distributed in Dechang and Xide counties, while low-risk areas were most prevalent in Xichang city and Mianning country. (4) Rainfall, temperature, elevation, and NPV emerged as the main influencing factors, exerting a dominant role in the occurrence of forest fires. Specifically, a higher NPV coverage correlates with an increased risk of forest fire. In conclusion, this study represents a novel approach by incorporating NPV and PV as key factors in triggering forest fires. By mapping forest-fire risk, we have provided a robust scientific foundation and decision-making support for effective fire management strategies. This research significantly contributes to advancing ecological civilization and fostering sustainable development.
Development of Oleogel-in-Water High Internal Phase Emulsions with Improved Physicochemical Stability and Their Application in Mayonnaise
Authors: Yu Jingjing , Yun Mingyue , Li Jia , Gao Yanxiang , Mao Like
Published: August 2024

Egg-free mayonnaise is receiving greater attention due to its potential health benefits. This study used whey protein isolate (WPI) as an emulsifier to develop high internal phase emulsions (HIPEs) based on beeswax (BW) oleogels through a simple one-step method. The effects of WPI, NaCl and sucrose on the physicochemical properties of HIPEs were investigated. A novel simulated mayonnaise was then prepared and characterized. Microstructural observation revealed that WPI enveloped oil droplets at the interface, forming a typical O/W emulsion. Increase in WPI content led to significantly enhanced stability of HIPEs, and HIPEs with 5% WPI had the smallest particle size (11.9 ± 0.18 μm). With the increase in NaCl concentration, particle size was increased and ζ-potential was decreased. Higher sucrose content led to reduced particle size and ζ-potential, and slightly improved stability. Rheological tests indicated solid-like properties and shear-thinning behaviors in all HIPEs. The addition of WPI and sucrose improved the structures and viscosity of HIPEs. Simulated mayonnaises (WE-0.3%, WE-1% and YE) were then prepared based on the above HIPEs. Compared to commercial mayonnaises, the mayonnaises based on HIPEs exhibited higher viscoelastic modulus and similar tribological characteristics, indicating the potential application feasibility of oleogel-based HIPEs in mayonnaise. These findings provided insights into the development of novel and healthier mayonnaise alternatives.
Poly(vinyl chloride) Films Incorporated with Antioxidant ZnO-Flavonoid Nanoparticles: A Strategy for Food Preservation
Authors: Braga Lilian R. , Oliveira Maria Graciele , Pérez Leonardo M. , Rangel Ellen T. , Machado Fabricio
Published: August 2024

Antioxidant films were prepared using poly(vinyl chloride) (PVC) incorporated with 0.5% or 1.0% zinc oxide (ZnO)-flavonoid (quercetin or morin) nanoparticles (NPZnO-Q% or NPZnO-M%) via the casting method. NP incorporation within the polymer matrix influenced the structural, morphological, optical, and thermal properties of the PVC-based films, as well as their antioxidant activity as assessed using the DPPH radical scavenging method. Our results indicated that increasing ZnO-flavonoid NP concentration increased films thickness, while reducing ultraviolet light (UV) transmittance but conserving transparency. The presence of NPZnO-Q% or NPZnO-M% improved the surface uniformity and thermal stability of the active films. In terms of antioxidant activity, there was an enhancement in the DPPH radical scavenging capacity (PVC/ZnO-Q1.0% > PVC/ZnO-Q0.5% > PVC/ZnO-M0.5% > PVC/ZnO-M1.0% > PVC), suggesting that the packaging can help protect food from oxidative processes. Therefore, these antioxidant films represent an innovative strategy for using as active food packaging material, especially intended for aiding in quality preservation and extending the shelf life of fatty foods.
Assessing the Influence of Cumulative Chlorella vulgaris Intake on Broiler Carcass Traits, Meat Quality and Oxidative Stability
Authors: Mendes Ana R. , Spínola Maria P. , Lordelo Madalena , Prates José A. M.
Published: August 2024

The impacts of cumulative Chlorella vulgaris intake (proportion of microalga in the diet multiplied by the total feed consumed by each bird) on broiler carcass traits, meat quality and oxidative stability were reviewed to identify the optimal intake levels for maximising benefits. Our findings indicate that a cumulative intake of 8.73 g/bird significantly enhances thigh yield, while levels ranging from 8.73 to 401 g/bird optimise carcass weight and overall meat quality. However, higher cumulative levels may reduce carcass dressing percentage due to metabolic inefficiencies. Furthermore, C. vulgaris intake improves the oxidative stability of broiler meat by increasing antioxidant levels and balancing pro- and antioxidants. Including C. vulgaris in broiler diets boosts total carotenoid content, and antioxidant assays confirm that it enhances meat oxidative stability, with low to moderate cumulative intake levels (8.73 to 401 g/bird) providing the best balance of benefits. Optimal oxidative stability and antioxidant properties were observed at a cumulative intake level of 401 g/bird, showing significant improvements in meat antioxidant capacity. Higher levels may lead to diminishing returns or potential negative effects due to the digestibility issues of the microalga. Future research should refine intake models, understand the bioavailability of C. vulgaris nutrients and explore cost-effective methods to enhance its digestibility, to ensure its viability and sustainability as a feed additive.
Mineral Concentrations in Different Types of Honey Originating from Three Regions of Continental Croatia
Authors: Tlak Gajger Ivana , Pavliček Damir , Oreščanin Višnja , Varenina Ivana , Sedak Marija , Bilandžić Nina
Published: August 2024

Honey has been recognized as a reliable indicator of environmental quality because of honeybees’ intense foraging activity, which brings them into contact with many persistent organic pollutants around the hive. In this study, four types of honey (meadow, acacia, chestnut, and honey in comb) collected at three different locations were analyzed for Co, Cr, Cu, Fe, Mn, Pb, and Zn levels. The highest levels of Fe and Cu in chestnut honey, Co and Zn in meadow honey, and Pb in honey in comb were observed in Varaždin County. The lowest levels of Pb in meadow honey and Co in comb honey were found from apiaries in Sisak-Moslavina County. Significant differences in the mean concentrations of Cr, Cu, Mn, and Fe were observed among the four honey types. Conversely, no significant differences in Co, Pb, and Zn levels were found. Most of the significant differences between the elements are related to chestnut honey. While sampling location (Fe) and type of honey (Pb), or both (Cr and Zn), significantly influenced the concentrations of some elements, these factors were found to be irrelevant for Mn, Co, and Cu. The results showed varying degrees of similarities and differences in mineral levels in honey samples, depending on floral and geographical origin.
Study on the Extraction of Nervonic Acid from the Oil of Xanthoceras sorbifolium Bunge Seeds
Authors: Gao Hui , Sun Jie , Guo Xuan , Zhang Ziyan , Liu He , Zhang Zhiran , Liu Mengkai , Zhou Sen , Li Shengxin , Zhang Tingting
Published: August 2024

Seven fatty acids were detected by GC-MS in Xanthoceras sorbifolium Bunge seed oil extracted at different temperatures, including Palmitic acid C16:0, Stearic acid C18:0, Oleic acid C18:1, Eicosenoic acid C20:1, Docosenoic acid C22:1, Tetracosenoic acid C24:1, and Linoleic acid C18:2. The highest content of nervonic acid (NA) was found in Xanthoceras sorbifolium Bunge seed oil extracted at 70 °C. Three methods were selected to analyze the extraction rate of nervonic acid in Xanthoceras sorbifolium Bunge seed oil, including urea complexation, low-temperature solvent crystallization, and a combined treatment using these two methods. The final content of nervonic acid obtained was 14.07%, 19.66%, and 40.17%, respectively. The combined treatment method increased the purity of nervonic acid in Xanthoceras sorbifolium Bunge seed oil by 12.62 times. Meanwhile, thermogravimetric behavior analysis of samples extracted using different methods was conducted by thermogravimetric analyzer, which suggested that the thermal stability of the samples extracted by the combined treatment was enhanced. These results can provide a new process parameter and scientific basis for the extraction of NA. At the same time, FTIR and NMR were also used to characterize the combined extraction sample, and the structure of the samples was proved.
Genome-Wide Survey of the Potential Function of CrLBDs in Catharanthus roseus MIA Biosynthesis
Authors: Chang Chunhao , Yang Bingrun , Guo Xiaorui , Gao Chunyan , Wang Biying , Zhao Xiaoju , Tang Zhonghua
Published: August 2024

Catharanthus roseus (C. roseus) can produce over 150 types of monoterpenoid indole alkaloids (MIAs), including vinblastine and vincristine, which are currently the primary sources of these alkaloids. Exploring the complex regulatory mechanisms of C. roseus is significant for resolving MIA biosynthesis. The Lateral Organ Boundaries Domain (LBD) is a plant-specific transcription factor family that plays crucial roles in the physiological processes of plant growth, stress tolerance, and specialized metabolism. However, the LBD gene family has not been extensively characterized in C. roseus, and whether its members are involved in MIA biosynthesis is still being determined. A total of 34 C. roseus LBD (CrLBD) genes were identified. RNA-Seq data were investigated to examine the expression patterns of CrLBD genes in various tissues and methyl jasmonate (MeJA) treatments. The results revealed that the Class Ia member CrLBD4 is positively correlated with iridoid biosynthetic genes (p < 0.05, r ≥ 0.8); the Class IIb member CrLBD11 is negatively correlated with iridoid biosynthetic genes (p < 0.05, r ≤ −0.8). Further validation in leaves at different growth stages of C. roseus showed that CrLBD4 and CrLBD11 exhibited different potential expression trends with iridoid biosynthetic genes and the accumulation of vindoline and catharanthine. Yeast one-hybrid (Y1H) and subcellular localization assays demonstrated that CrLBD4 and CrLBD11 could bind to the “aattatTCCGGccgc” cis-element and localize to the nucleus. These findings suggest that CrLBD4 and CrLBD11 may be potential candidates for regulating MIA biosynthesis in C. roseus. In this study, we systematically analyzed the CrLBD gene family and provided insights into the roles of certain CrLBDs in the MIA biosynthesis of C. roseus.
The Development of the Adaptive Behavior Scale for Stroke Survivors
Authors: Choi Hyunsuk , Song Youngshin
Published: August 2024

The adjustment of stroke survivors is self-directed and multi-dimensional. This study developed an adaptive behavior scale for stroke survivors reflecting these characteristics and performed a psychometric evaluation. The item pool was derived based on conceptual attributes and indicators of adaptive behaviors for stroke survivors. Ten experts assessed the content validity. The scale was refined through pilot testing and interviews with 10 stroke survivors. From December 2021 to May 2022, a self-report questionnaire consisting of a five-point Likert scale was administered to 215 stroke survivors visiting a university hospital in S City, South Korea. Item analysis and confirmatory factor analysis were conducted to assess the construct validity; reliability was confirmed using Cronbach’s α. The final scale comprised three factors and 16 items: taking an optimistic view, restructuring daily activities to suit oneself, and carrying out one’s daily life. The confirmatory factor analysis indicated a good fit for the three-factor model; Cronbach’s α coefficient of the scale was 0.90, indicating a very good internal consistency. This easy-to-use, concise self-report scale applies to stroke survivors from subacute to chronic stages, providing healthcare professionals with the basic data needed to assess their adaptation. It may also facilitate individualized intervention program development to improve stroke survivor adaptation.
A Cross-Sectional Study of Spanish Grindr Users: Sociodemographic Characteristics, Sexual Health Trends, and Attitudes toward HIV Testing
Authors: Ibáñez-Tomás Eduardo , Gasch-Gallén Ángel
Published: August 2024

Men who have sex with men are at an increased risk of acquiring sexually transmitted infections, and although behavioral and contextual interventions have improved, infections continue to spread. Therefore, a new focus on recent trends in sexual health in this population is needed. The aim of this study was to describe the relationship between sociodemographic and behavioral characteristics according to Grindr usage patterns, the prevalence of sexually transmitted infections (STIs), and attitudes toward HIV testing. In January 2020, a cross-sectional study was conducted using a sample of 881 men who have sex with men (MSM) who completed an online questionnaire. We evaluated their Grindr use patterns (moderate or intensive) and explored the associations of these with sociodemographic behavioral characteristics, STIs, and attitudes toward HIV testing. Of 881 participants in total, 587 (66.6%) were intensive Grindr users. Compared to moderate users, these participants reported significantly higher numbers of casual sexual partners (76.2% vs. 23.8%; p < 0.001), were more likely to participate in Chemsex (77.3% vs. 22.7%: p = 0.031), and had a higher self-reported number of STIs (69.9% vs. 30.1; p = 0.046). They also demonstrated better attitudes toward HIV testing (69.7% vs. 30.3%; p = 0.045) and perceived themselves to be at moderate risk of HIV (71.2% vs. 28.8%: p = 0.048). Moreover, intensive Grindr users were 1.36 times more likely than moderate users to test positive for STIs (95%CI = 1.15–1.91; p = 0.048). With some limitations, age, sexual partners, intensive Grindr users, and PrEP uses were associated with the risk of STIs in the sample of Spanish Grindr users studied. As Grindr users are vulnerable to sociodemographic and behavioral factors that determine STIs and HIV infection, mainly among intensive users, it is necessary to highlight the importance of new trends such as online dating apps, PrEP use, substance use in sex, and Chemsex, and these need to be incorporated into online public health strategies.
Collisions and Perceptions of Cyberbullying: Comparison of Intergenerational Experiences
Authors: Soldatova Galina , Chigarkova Svetlana , Rasskazova Elena
Published: August 2024

With regard to negative consequences, cyberbullying is recognized as one of the most traumatic types of cyber aggression. The aim is to study the specific features of adolescents and youth’s cyberbullying experience in the role of an aggressor, victim or bystander, as well as awareness on the part of parents of adolescents. A total of 3395 adolescents, youth and parents filled out specially designed questionnaires. Older adolescents turned out to be at higher risk of cyberbullying. In two-thirds of cases, cyberbullying is related to real-life incidents. Aggressors are motivated by domination and entertainment, primarily employing strategies of social exclusion, harassment and denigration. As victims of cyberbullying, younger adolescents turn to their parents and friends for social support, whereas older adolescents and young adults are more likely turn to their peers. In the role of a bystander, almost half of younger adolescents and about a third of older adolescents and young adults choose the prosocial strategy of protecting a victim. The parents often underestimate the experience of encountering cyberbullying or find it difficult to assess such experience. The identified risk groups and strategies and the lack of parents’ awareness are important to take into account when drawing up cyberbullying prevention programs.
Advancements in Plant-Based Therapeutics for Hepatic Fibrosis: Molecular Mechanisms and Nanoparticulate Drug Delivery Systems
Authors: Ciceu Alina , Fenyvesi Ferenc , Hermenean Anca , Ardelean Simona , Dumitra Simona , Puticiu Monica
Published: August 2024

Chronic liver injuries often lead to hepatic fibrosis, a condition characterized by excessive extracellular matrix accumulation and abnormal connective tissue hyperplasia. Without effective treatment, hepatic fibrosis can progress to cirrhosis or hepatocellular carcinoma. Current treatments, including liver transplantation, are limited by donor shortages and high costs. As such, there is an urgent need for effective therapeutic strategies. This review focuses on the potential of plant-based therapeutics, particularly polyphenols, phenolic acids, and flavonoids, in treating hepatic fibrosis. These compounds have demonstrated anti-fibrotic activities through various signaling pathways, including TGF-β/Smad, AMPK/mTOR, Wnt/β-catenin, NF-κB, PI3K/AKT/mTOR, and hedgehog pathways. Additionally, this review highlights the advancements in nanoparticulate drug delivery systems that enhance the pharmacokinetics, bioavailability, and therapeutic efficacy of these bioactive compounds. Methodologically, this review synthesizes findings from recent studies, providing a comprehensive analysis of the mechanisms and benefits of these plant-based treatments. The integration of novel drug delivery systems with plant-based therapeutics holds significant promise for developing effective treatments for hepatic fibrosis.
Transcriptomic Insights into Salt Stress Response in Two Pepper Species: The Role of MAPK and Plant Hormone Signaling Pathways
Authors: Farooq Muhammad Aamir , Zeeshan Ul Haq Muhammad , Zhang Liping , Wu Shuhua , Mushtaq Naveed , Tahir Hassam , Wang Zhiwei
Published: August 2024

Salt stress imposes significant plant limitations, altering their molecular, physiological, and biochemical functions. Pepper, a valuable herbaceous plant species of the Solanaceae family, is particularly susceptible to salt stress. This study aimed to elucidate the physiological and molecular mechanisms that contribute to the development of salt tolerance in two pepper species (Capsicum baccatum (moderate salt tolerant) and Capsicum chinense (salt sensitive)) through a transcriptome and weighted gene co-expression network analysis (WGCNA) approach to provide detailed insights. A continuous increase in malondialdehyde (MDA) and hydrogen peroxide (H2O2) levels in C. chinense and higher activities of catalase (CAT), superoxide dismutase (SOD), and peroxidase (POD) in C. baccatum indicated more tissue damage in C. chinense than in C. baccatum. In transcriptome analysis, we identified 39 DEGs related to salt stress. Meanwhile, KEGG pathway analysis revealed enrichment of MAPK and hormone signaling pathways, with six DEGs each. Through WGCNA, the ME.red module was identified as positively correlated. Moreover, 10 genes, A-ARR (CQW23_24856), CHIb (CQW23_04881), ERF1b (CQW23_08898), PP2C (CQW23_15893), ABI5 (CQW23_29948), P450 (CQW23_16085), Aldedh1 (CQW23_06433), GDA (CQW23_12764), Aldedh2 (CQW23_14182), and Aldedh3 (CQW23_11481), were validated by qRT-PCR. This study provides valuable insights into the genetic mechanisms underlying salt stress tolerance in pepper. It offers potential targets for future breeding efforts to enhance salt stress resilience in this crop.
A Cautionary Tale of Hypertrophic Cardiomyopathy—From “Benign” Left Ventricular Hypertrophy to Stroke, Atrial Fibrillation, and Molecular Genetic Diagnostics: A Case Report and Review of Literature
Authors: Gencheva Dolina , Angelova Petya , Genova Kameliya , Atemin Slavena , Sleptsova Mila , Todorov Tihomir , Nikolov Fedya , Ruseva Donka , Mitev Vanyo , Todorova Albena
Published: August 2024

This case report concerns a 48-year-old man with a history of ischemic stroke at the age of 41 who reported cardiac hypertrophy, registered in his twenties when explained by increased physical activity. Family history was positive for a mother with permanent atrial fibrillation from her mid-thirties. At the age of 44, he had a first episode of persistent atrial fibrillation, accompanied by left atrial thrombosis while on a direct oral anticoagulant. He presented at our clinic at the age of 45 with another episode of persistent atrial fibrillation and decompensated heart failure. Echocardiography revealed a dilated left atrium, reduced left ventricular ejection fraction, and an asymmetric left ventricular hypertrophy. Cardiac magnetic resonance was positive for a cardiomyopathy with diffuse fibrosis, while slow-flow phenomenon was present on coronary angiography. Genetic testing by whole-exome sequencing revealed three variants in the patient, c.309C > A, p.His103Gln in the ACTC1 gene, c.116T > G, p.Leu39Ter in the PLN gene, and c.5827C > T, p.His1943Tyr in the SCN5A gene, the first two associated with hypertrophic cardiomyopathy and the latter possibly with familial atrial fibrillation. This case illustrates the need for advanced diagnostics in unexplained left ventricular hypertrophy, as hypertrophic cardiomyopathy is often overlooked, leading to potentially debilitating health consequences.
Data-Driven Modelling of Substituted Pyrimidine and Uracil-Based Derivatives Validated with Newly Synthesized and Antiproliferative Evaluated Compounds
Authors: Zukić Selma , Osmanović Amar , Harej Hrkać Anja , Kraljević Pavelić Sandra , Špirtović-Halilović Selma , Veljović Elma , Roca Sunčica , Trifunović Snežana , Završnik Davorka , Maran Uko
Published: August 2024

The pyrimidine heterocycle plays an important role in anticancer research. In particular, the pyrimidine derivative families of uracil show promise as structural scaffolds relevant to cervical cancer. This group of chemicals lacks data-driven machine learning quantitative structure-activity relationships (QSARs) that allow for generalization and predictive capabilities in the search for new active compounds. To achieve this, a dataset of pyrimidine and uracil compounds from ChEMBL were collected and curated. A workflow was developed for data-driven machine learning QSAR using an intuitive dataset design and forwards selection of molecular descriptors. The model was thoroughly externally validated against available data. Blind validation was also performed by synthesis and antiproliferative evaluation of new synthesized uracil-based and pyrimidine derivatives. The most active compound among new synthesized derivatives, 2,4,5-trisubstituted pyrimidine was predicted with the QSAR model with differences of 0.02 compared to experimentally tested activity.
The Impact of the Endocrine and Immunological Function of Adipose Tissue on Reproduction in Women with Obesity
Authors: Mączka Katarzyna , Stasiak Olga , Przybysz Paulina , Grymowicz Monika , Smolarczyk Roman
Published: August 2024

Obesity, which leads to metabolic dysregulation and body function impairment, emerges as one of the pressing health challenges worldwide. Excessive body fat deposits comprise a dynamic and biologically active organ possessing its own endocrine function. One of the mechanisms underlying the pathophysiology of obesity is low-grade systemic inflammation mediated by pro-inflammatory factors such as free fatty acids, lipopolysaccharides, adipokines (including leptin, resistin and visfatin) and cytokines (TNF-α, IL-1β, Il-6), which are secreted by adipose tissue. Together with obesity-induced insulin resistance and hyperandrogenism, the exacerbated immune response has a negative impact on the hypothalamic–pituitary–gonadal axis at all levels and directly affects reproduction. In women, it results in disrupted ovarian function, irregular menstrual cycles and anovulation, contributing to infertility. This review focuses on the abnormal intracellular communication, altered gene expression and signaling pathways activated in obesity, underscoring its multifactorial character and consequences at a molecular level. Extensive presentation of the complex interplay between adipokines, cytokines, immune cells and neurons may serve as a foundation for future studies in search of potential sites for more targeted treatment of reproductive disorders related to obesity.
Cross-Linked Thiolated Hydroxypropil-β-Cyclodextrin for Pulmonary Drug Delivery
Authors: Cerri Luca , Migone Chiara , Vizzoni Lucia , Grassiri Brunella , Fabiano Angela , Piras Anna Maria , Zambito Ylenia
Published: August 2024

Inhalable formulations with cyclodextrins (CDs) as solubility and absorption enhancers show promise for pulmonary delivery. Thiolated hydroxypropyl-β-cyclodextrin (HP-β-CD-SH) has mucoadhesive properties, enhancing drug absorption. Moreover, it has self-aggregation capability, which could further improve absorption and drug stability, as well as reduce irritation. This study aims to stabilize CD nanoaggregates using bifunctional cross-linkers and evaluate their benefits for lung drug delivery compared to pristine HP-β-CD-SH. Methods: The effectiveness of cross-linked HP-β-CD-SH nanoparticles (HP-β-CD-SH-NP) was compared to transient nanoaggregates in enhancing the activity of dexamethasone (DMS) and olive leaf extracts (OLE). DMS, a poorly soluble drug commonly used in lung treatments, and OLE, known for its antioxidant properties, were chosen. Drug-loaded HP-β-CD-SH-NP were prepared and nebulized onto a lung epithelial Air–Liquid Interface (ALI) model, assessing drug permeation and activity. Results: HP-β-CD-SH with 25% thiolation was synthesized via microwave reaction, forming 150 nm nanoaggregates and stabilized 400 nm HP-β-CD-SH-NP. All carriers showed good complexing ability with DMS and OLE and were biocompatible in the lung ALI model. HP-β-CD-SH promoted DMS absorption, while stabilized HP-β-CD-SH-NP protected against oxidative stress. Conclusion: HP-β-CD-SH is promising for lung delivery, especially as stabilized nanoaggregates, offering versatile administration for labile molecules like natural extracts.
A Review of Animal Models for Studying Bone Health in Type-2 Diabetes Mellitus (T2DM) and Obesity
Authors: Norazman Saiful Iqbal , Mohd Zaffarin Anis Syauqina , Shuid Ahmad Nazrun , Hassan Haniza , Soleiman Ima Nirwana , Kuan Wong Sok , Alias Ekram
Published: August 2024

Preclinical research on diabetes and obesity has been carried out in various animal models over the years. These animal models are developed from genetic manipulation that affects their body metabolism, chemical-induced procedures, diet alteration/modifications, or combinations of the aforementioned approaches. The diabetic and obesity animal models have allowed researchers to not only study the pathological aspect of the diseases but also enable them to screen and explore potential therapeutic compounds. Besides several widely known complications such as macrovascular diseases, diabetic neuropathy, nephropathy and retinopathy, type 2 diabetes mellitus is also known to affect bone health. There is also evidence to suggest obesity affects bone health. Therefore, continuous research needs to be conducted to find a remedy or solution to this matter. Previous literature reported evidence of bone loss in animal models of diabetes and obesity. These findings, as highlighted in this review, further augment the suggestion of an inter-relationship between diabetes, obesity and bone loss.
Hyaluronic Acid/Platelet-Rich Plasma Mixture Improves Temporomandibular Joint Biomechanics: A Systematic Review
Authors: Chęciński Maciej , Lubecka Karolina , Bliźniak Filip , Chlubek Dariusz , Sikora Maciej
Published: August 2024

Hyaluronic acid (HA) is the main component of the temporomandibular joint (TMJ) synovial fluid. Arthritis in temporomandibular disorders (TMDs) disrupts HA metabolism, resulting in shorter polymeric chain predominance and increased friction. Intra-articular injections of HA supplement the larger molecules of this glycosaminoglycan, and the platelet-rich plasma (PRP) delivered in this way releases growth factors, suppressing inflammation. This PRISMA-compliant PROSPERO-registered (CRD42024564382) systematic review aimed to assess the validity of mixing HA with PRP in the injectable treatment of TMJ disorders. We searched the medical literature for eligible randomized clinical trials using BASE, Google Scholar, PubMed and Scopus engines on 9 May 2024, with no time frame limit. Selected reports were assessed for risk of bias using the Cochrane RoB2 tool. Numerical data were collected on articular pain and mandibular mobility. We provided mean differences from baseline and between study and control groups at each observation point. The efficacy of TMD treatment with HA/PRP versus HA or PRP alone was assessed meta-analytically. Of 171 identified records, we selected 6 studies. In the 6-month follow-up, the mean advantage of PRP supplementation with HA was 2.52 (SE = 2.44; d = 0.83) mm and the benefit of adding PRP to HA was 1.47 (SE = 2.68; d = 0.34) mm in mandibular abduction. The pain-improvement scores were −1.33 (SE = 1.02; d = −1.05) and −1.18 (SE = 0.92; d = 0.80), respectively. Presumably, the HA/PRP range of therapeutic efficiency includes cases non-respondent to HA or PRP alone.
Constraint-Induced Movement Therapy (CIMT) and Neural Precursor Cell (NPC) Transplantation Synergistically Promote Anatomical and Functional Recovery in a Hypoxic-Ischemic Mouse Model
Authors: Rumajogee Prakasham , Altamentova Svetlana , Li Junyi , Puvanenthirarajah Nirushan , Wang Jian , Asgarihafshejani Azam , Van Der Kooy Derek , Fehlings Michael G.
Published: August 2024

Cerebral palsy (CP) is a common neurodevelopmental disorder characterized by pronounced motor dysfunction and resulting in physical disability. Neural precursor cells (NPCs) have shown therapeutic promise in mouse models of hypoxic-ischemic (HI) perinatal brain injury, which mirror hemiplegic CP. Constraint-induced movement therapy (CIMT) enhances the functional use of the impaired limb and has emerged as a beneficial intervention for hemiplegic CP. However, the precise mechanisms and optimal application of CIMT remain poorly understood. The potential synergy between a regenerative approach using NPCs and a rehabilitation strategy using CIMT has not been explored. We employed the Rice–Vannucci HI model on C57Bl/6 mice at postnatal day (PND) 7, effectively replicating the clinical and neuroanatomical characteristics of hemiplegic CP. NPCs were transplanted in the corpus callosum (CC) at PND21, which is the age corresponding to a 2-year-old child from a developmental perspective and until which CP is often not formally diagnosed, followed or not by Botulinum toxin injections in the unaffected forelimb muscles at PND23, 26, 29 and 32 to apply CIMT. Both interventions led to enhanced CC myelination and significant functional recovery (as shown by rearing and gait analysis testing), through the recruitment of endogenous oligodendrocytes. The combinatorial treatment indicated a synergistic effect, as shown by newly recruited oligodendrocytes and functional recovery. This work demonstrates the mechanistic effects of CIMT and NPC transplantation and advocates for their combined therapeutic potential in addressing hemiplegic CP.