bluuebunny/crossref_metadata_2025 huggingface dataset:
Financial Distress, Firm Life Cycle, and Corporate Restructuring Decisions: Evidence from Pakistan’s Economy
Authors: Akbar Minhas , Hussain Ammar , Sokolova Marcela , Sabahat Tanazza
Published: July 2022

This study examines the influence of financial distress on corporate restructuring decisions and whether this restructuring varies across the Firm Life Cycle (FLC) stages of Pakistani non-financial listed firms for the 12 years from 2005 to 2016 inclusive. FLC stages and financial distress are measured using the Dickinson model and Altman Z-score, respectively. Corporate restructuring is segregated into equity and debt restructuring. The data are analyzed using a panel logistic regression model. The results reveal that financial distress is negatively associated with corporate debt restructuring decisions and positively associated with corporate equity restructuring decisions. Further analysis shows that new, growing and mature firms have positive associations with equity restructuring decisions and negative associations with debt restructuring decisions, while declining firms prefer debt restructuring. This study has important implications for corporate managers and policy makers.
Residual Forest Biomass in Pinus Stands: Accumulation and Biogas Production Potential
Authors: Eftaxias Alexandros , Passa Evangelia Anna , Michailidis Christos , Daoutis Christodoulos , Kantartzis Apostolos , Diamantis Vasileios
Published: July 2022

Lignocellulosic biomass is an abundant resource that can be valorized for the production of bioenergy. However, studies aiming to quantify the amount of biogas production potential per km forest road are scarce in the literature. In this study, fresh pine needles, pine needle litter, pine branches, and pine bark were digested in batch reactors under mesophilic conditions after a grinding/milling pre-treatment. All samples were collected from a low-altitude Mediterranean Pinus forest (North Greece) adjacent to a category G forest road with a gentle slope. The methane yield of fresh pine needles was between 115 and 164 NmL g−1 volatile solids (VS), depending on the Pinus tree size. Pine needle litter produced a significantly lower methane yield (between 58 and 77 NmL g−1 VS), followed by pine bark (85 NmL g−1 VS) and pine branches (138 NmL g−1 VS). Considering the quantity of pine needle litter accumulated on adjacent forest roads (600 ± 200 g m−2), it was possible to calculate the biomethane production potential per km of forest road (up to 500 Nm3 km−1) if the biomass collected was disposed of at an anaerobic digestion facility. The results of the study demonstrate that residual forest biomass represents an additional resource for bioenergy production. Moreover, harvesting residual forest biomass can decrease the incidence of devastating summer forest fires and their disastrous consequences for the environment, the economy, and the local populations.
Multi-Objective Constructal Design for Square Heat-Generation Body with “Arrow-Shaped” High-Thermal-Conductivity Channel
Authors: Zhu Hongwei , Chen Lingen , Ge Yanlin , Shi Shuangshuang , Feng Huijun
Published: July 2022

Based on the square heat-generation body (HGB) with “arrow-shaped” high-thermal-conductivity channel (HTCC) model established in the previous literature, we performed multi-objective optimization (MOO) with maximum temperature difference (MTD) minimization and entropy-generation rate (EGR) minimization as optimization objectives for its performance. Pareto frontiers with optimal set were obtained based on NSGA-II. TOPSIS, LINMAP, and Shannon entropy decision methods were used to select the optimal results in Pareto frontiers, and the deviation index was used to compare and analyze advantages and disadvantages of the optimal results for each decision method. At the same time, multi-objective constructal designs of the “arrow-shaped” HTCC were carried out through optimization of single degree of freedom (DOF), two DOF, and three DOF, respectively, and the thermal performance of the square heat-generation body under optimizations of different DOF were compared. The results show that constructal design with the MOO method can achieve the best compromise between the maximum thermal resistance and the irreversible loss of heat transfer of the square heat-generation body, thereby improving the comprehensive thermal performance of the square heat-generation body. The MOO results vary with different DOF, and optimization with increasing DOF can further improve the comprehensive thermal performance of square HGBs.
Multi-Objective Energy Efficient Adaptive Whale Optimization Based Routing for Wireless Sensor Network
Authors: Bali Himani , Gill Amandeep , Choudhary Abhilasha , Anand Divya , Alharithi Fahd , Aldossary Sultan , Mazón Juan
Published: July 2022

In Wireless Sensor Networks (WSNs), routing algorithms can provide energy efficiency. However, due to unbalanced energy consumption for all nodes, the network lifetime is still prone to degradation. Hence, energy efficient routing was developed in this article by selecting cluster heads (CH) with the help of adaptive whale optimization (AWOA) which was used to reduce time-consumption delays. The multi-objective function was developed for CH selection. The clusters were then created using the distance function. After establishing groupings, the supercluster head (SCH) was selected using the benefit of a fuzzy inference system (FIS) which was used to collect data for all CHs and send them to the base station (BS). Finally, for the data-transfer procedure, hop count routing was used. An Oppositional-based Whale optimization algorithm (OWOA) was developed for multi-constrained QoS routing with the help of AWOA. The performance of the proposed OWOA methodology was analyzed according to the following metrics: delay, delivery ratio, energy, NLT, and throughput and compared with conventional techniques such as particle swarm optimization, genetic algorithm, and Whale optimization algorithm.
Economic Conditions of Using Biodegradable Waste for Biogas Production, Using the Example of Poland and Germany
Authors: Sobczak Anna , Chomać-Pierzecka Ewa , Kokiel Andrzej , Różycka Monika , Stasiak Jacek , Soboń Dariusz
Published: July 2022

Biogas production is a process with great potential. It uses the biodegradable raw materials of animal, vegetable and municipal waste. The amount of municipal as well as agricultural waste is increasing every year. This waste is an unmanaged and nuisance waste, and using it in biogas plants reduces the amount of waste. Biogas production is part of the EU’s policy to reduce dependence on fossil fuels and use energy from renewable sources (diversification of energy sources). Its importance is certain to increase in the future as energy demand increases. This article deals with the economical use of biodegradable waste for biogas production in Poland and Germany. Both countries have a similar agricultural and municipal waste structure. An agricultural biogas plant is one way of obtaining energy based on renewable energy sources (RES). Energy production from agricultural biogas will allow Poland to meet the 32% obligation imposed by the EU and Germany to continue to be the market leader in biogas plants. The biogas market in Poland is growing, while in Germany, there is a decline in biogas installations. The article indicates what changes need to take place in agriculture and the use of municipal waste in these countries to sustain the development of biogas plants. Both countries should maintain animal husbandry to ensure continuous access to substrate and use waste for production rather than growing maize or other mixtures only for biogas plants. Due to the high price of chemical fertilisers, pulp from biogas plants should be an alternative to chemical fertilisers in both countries, which will contribute to greener crops. The governments of both countries should support such measures.
Energy Potential of Biogas from Sewage Sludge after Thermal Hydrolysis and Digestion
Authors: Mukawa Jakub , Pająk Tadeusz , Rzepecki Tadeusz , Banaś Marian
Published: July 2022

This paper presents the energy potential of biogas obtained from the anaerobic digestion (AD) of sewage sludge (SS) preceded by thermal hydrolysis (THP) using Cambi THP® technology. The presented data are for the Tarnów (Poland) wastewater treatment plant for the year 2020. A detailed energy balance of biogas and its use in the cogeneration process and in the production of heat in the water boiler and the steam boiler is presented. The article contains data on the amount of processed SS and the content of dry matter and dry organic matter at different stages of the technological process. The annual plant operation resulted in the production of 3,276,497 Nm3 of biogas as a result of processing 8684 tonnes of dry solids (tDS) of municipal SS from the Tarnów wastewater treatment plant (WWTP) and regional WWTPs. The energy potential of the produced biogas was 75,347.06 GJ. The average calorific value of biogas was 23,021 kJ/Nm3.. The obtained biogas production allowed us to cover the thermal energy demand of the THP 100%. The annual average specific biogas conversion rate during the study period was 0.761 Nm3/kg of dry organic matter reduced and the average organic matter content reduction in the sludge was 64.60%.
Novel High-Step-Up/Step-Down Three-Port Bidirectional DC/DC Converter for Photovoltaic Systems
Authors: Wu Yu-En
Published: July 2022

This paper presents a novel three-port high-step-up/step-down bidirectional DC/DC converter with a coupled inductor for photovoltaic (PV) systems. The proposed converter combines a high-step-up converter, which is used to step-up the PV module to DC bus 200 V, and a battery charge/discharge bidirectional converter to form a three-port bidirectional converter. When sufficient energy is supplied from the PV modules, the converter can step-up the output of the PV modules and provide energy to the DC bus while charging the battery simultaneously. However, when no energy is supplied from the PV modules, the DC bus voltage is provided by the battery. Moreover, the energy stored in the leakage inductor is recycled to the DC-blocking capacitor, and synchronous rectification is conducted in the switch during the step-up mode to reduce switch loss and thereby increase the system’s overall efficiency. Finally, a 500 W three-port bidirectional converter is implemented to verify the feasibility and practicability of the proposed converter. The maximum efficiency of the proposed converter is 95.4%, 94.3%, and 94.7% when operated in the high-step-up mode, battery step-up mode, and step-down mode for the PV modules, respectively.
Demand Response Impact Evaluation: A Review of Methods for Estimating the Customer Baseline Load
Authors: Valentini Ottavia , Andreadou Nikoleta , Bertoldi Paolo , Lucas Alexandre , Saviuc Iolanda , Kotsakis Evangelos
Published: July 2022

Climate neutrality is one of the greatest challenges of our century, and a decarbonised energy system is a key step towards this goal. To this end, the electricity system is expected to become more interconnected, digitalised, and flexible by engaging consumers both through microgeneration and through demand side flexibility. A successful use of these flexibility tools depends widely on the evaluation of their effects, hence the definition of methods to assess and evaluate them is essential for their implementation. In order to enable a reliable assessment of the benefits from participating in demand response, it is necessary to define a reference value (“baseline”) to allow for a fair comparison. Different methodologies have been investigated, developed, and adopted for estimating the customer baseline load. The article presents a structured overview of methods for the estimating the customer baseline load, based on a review of academic literature, existing standardisation efforts, and lessons from use cases. In particular, the article describes and focuses on the different baseline methods applied in some European H2020 projects, showing the results achieved in terms of measurement accuracy and costs in real test cases. The most suitable methodology choice among the several available depends on many factors. Some of them can be the function of the Demand Response (DR) service in the system, the broader regulatory framework for DR participation in wholesale markets, or the DR providers characteristics, and this list is not exclusive. The evaluation shows that the baseline methodology choice presents a trade-off among complexity, accuracy, and cost.
A Shale Gas Leaking Incident in Fuling Shale Gas Field in Chongqing, China: A Case Study
Authors: Zhang Ye , Mao Haijun , Zhang Zhiping , Jiang Shu , Liu Yiming
Published: July 2022

A ground natural gas leaking event at the Fuling shale gas field is reported in this paper. Thirteen leakage spots were discovered in two places near the SW-1 and SW-2 drilling wells. The biggest leak rate was above 1000 m3 per day, and no H2S was identified in any of the 13 leaking spots, according to the field study. The chemical components, carbon isotope properties of the leaking gases, and the geological context of the leaking location were researched in order to determine the reason for the leaking incident. From the geological conditions, the Shimen 1# fault belt cuts the whole strata from bottom to top, according to seismic reverse time migration (RTM), and the fault and leaking spots are located in the range of the surface projection of the horizontal section of the adjacent shale gas well. The fracture development evaluation shows that the Wufeng–Longmaxi Formation, which is also the producing layer of the Fuling shale gas field, has a very high possibility of fracture development. From the geochemical view, the carbon isotope of the leaking gases lies in the range of the Wufeng–Longmaxi Formation, showing that the Wufeng–Longmaxi Formation is the gas source of the leaking gas.
Estimating the Emissions Reduction Due to the Use of Cargo Bikes: Case Studies for the Selected European Cities
Authors: Vasiutina Hanna , Naumov Vitalii , Szarata Andrzej , Rybicki Stanisław
Published: July 2022

Contemporary cities need new solutions to reduce the harmful impact of freight transport, such as traffic congestion, air pollution, and noise emissions, which have increased in the last few years. This especially concerns central parts of cities due to the architectural features of urban space. The concept of green logistics intends to replace currently used combustion engine vehicles with zero-emissions technologies such as the use of cargo bikes to deliver goods to final recipients located in the city districts with dense urban development. However, the use of cargo bikes for certain architectural, transport, and business conditions must be justified: the positive impact of that innovative means of transport should be evaluated and assessed by decision-makers. We propose a method to evaluate the environmental impact of cargo bikes that replace conventional vehicles in the central district of a city. Our approach assumes simulations of freight travel demand in the selected city area, the estimation of the part of trips to be replaced by cargo bikes, and, finally, the evaluation of the total distance covered by the replaced combustion engine vehicles to show the reduction of CO2 generated by the conventional vehicles. The case studies of the central districts for selected partner cities (Vitoria-Gasteiz, San Sebastian, Dubrovnik, and Mechelen) that participate in the CityChangerCargoBike project are shown to demonstrate the developed methodology.
On-Road and Laboratory Emissions from Three Gasoline Plug-In Hybrid Vehicles-Part 2: Solid Particle Number Emissions
Authors: Melas Anastasios , Selleri Tommaso , Franzetti Jacopo , Ferrarese Christian , Suarez-Bertoa Ricardo , Giechaskiel Barouch
Published: July 2022

Plug-in hybrid electric vehicles (PHEVs) are a promising technology for reducing the tailpipe emissions of CO2 as well as air pollutants, especially in urban environments. However, several studies raise questions over their after-treatment exhaust efficiency when their internal combustion engine (ICE) ignites. The rationale is the high ICE load during the cold start in combination with the cold conditions of the after-treatment devices. In this study, we measured the solid particle number (SPN) emissions of two Euro 6d and one Euro 6d-TEMP gasoline direct injection (GDI) PHEVs (electric range 52–61 km) all equipped with a gasoline particulate filter, in the laboratory and on-road with different states of charge of the rechargeable electric energy storage system (REESS) and ambient temperatures. All vehicles met the regulation limits but it was observed that, even for fully charged REESS, when the ICE ignited SPN emissions were similar or even higher in some cases compared to the operation of these vehicles solely with their ICE (discharged REESS) and also when compared to conventional GDI vehicles. On-road SPN emission rate spikes during the first 30 s after a cold start were, on average, 2 to 15 times higher with charged compared to discharged REESS due to higher SPN concentrations and exhaust flow rates. For one vehicle in the laboratory under identical driving conditions, the ICE ignition at high load resulted in 10-times-higher SPN emission rate spikes at cold-start compared to hot-start. At −10 °C, for all tested vehicles, the ICE ignited at the beginning of the cycle even when the REESS was fully charged, and SPN emissions increased from 30% to 80% compared to the cycle at 23 °C in which the ICE ignited. The concentration of particles below 23 nm, which is the currently regulated lower particle size, was low (≤18%), showing that particles larger than 23 nm were mainly emitted irrespective of cold or hot engine operation and ambient temperature.
New Taxonomy of Climate Adaptive Building Shell Office Buildings: Focus on User–Façade Interaction Scenarios
Authors: Hafizi Nazgol , Vural Sadiye
Published: July 2022

As one of the most critical considerations in the contemporary era, sustainability heightens the need to find more suitable solutions for architectural designs. Climate adaptive building shells (CABS) are among the most promising alternatives for achieving sustainability goals by reducing energy consumption. Regardless of technological developments, this type of system has a reputation for increasing the distraction of occupants and consequently decreasing their satisfaction level. This research has been developed to focus on the occupant-centric study rather than technological advancements of the system. This study introduces the user–façade interaction scenarios and applies this classification on CABS office buildings. The purpose of this study is to introduce a new multi-domain taxonomy for CABS office buildings and update the database of this system by adding a new variable focusing on occupants. The study was designed on the foundation found with PRISMA methodology which highlights the lack of occupant-centric research on CABS. The research carried on as a qualitative method with an inductive approach which with the literature review introduced the user–façade interaction scenarios and the latest update of the CABS database. Accordingly, the office cases were categorized within different climatic zones, and later as a correlational study, each case was studied based on user–façade interaction scenarios. Analysis of case databases according to user–façade interaction types clears the lack of development in the majority of scenarios. Lastly, the study concluded by introducing a novel multi-domain taxonomy of CABS office buildings by considering user–façade interaction scenarios. The further value of this study is to be a foundation for future studies on CABS office buildings by considering the occupants as a primary element of the research.
Artificial Electric Field Algorithm-Pattern Search for Many-Criteria Networks Reconfiguration Considering Power Quality and Energy Not Supplied
Authors: Alanazi Abdulaziz , Alanazi Mohana
Published: July 2022

Considering different objectives and using powerful optimization methods in the distribution networks reconfiguration by accurately achieving the best network configuration can further improve network performance. In this paper, reconfiguration of radial distribution networks is performed to minimize the power loss, voltage sag, voltage unbalance, and energy not supplied (ENS) of customers using a new intelligent artificial electric field algorithm-pattern search (AEFAPS) method based on the many-criteria optimization approach. The voltage sag and voltage unbalance are defined as power quality indices and the ENS is the reliability index. In this study, the pattern search (PS) algorithm enhances the artificial electric field algorithm’s (AEFA) flexibility search both globally and locally. AEFAPS is applied to determine the decision variables as open switches of the networks considering the objective function and operational constraints. The proposed methodology based on AEFAPS is performed on an unbalanced 33-bus IEEE standard network and a real unbalanced 13-bus network. The reconfiguration problem is implemented in single-criterion and many-criteria optimization approaches to evaluate the proposed methodology’s effectiveness using different algorithms. The single-criterion results demonstrated that some power quality indices might be out of range, while all indices are within the permitted range in the many-criteria optimization approach, proving the effectiveness of the proposed many-criteria reconfiguration with logical compromise between different objectives. The results show that AEFAPS identified the network configuration optimally and different objectives are improved considerably compared to the base network. The results confirmed the superior capability of AEFAPS to obtain better objective values and lower values of losses, voltage sag, voltage unbalance, and ENS compared with conventional AEFA, particle swarm optimization (PSO), and grey wolf optimizer (GWO). Moreover, the better performance of AEFAPS is proved in solving the reconfiguration problem compared with previous studies.
A 10 kV/1 MW High-Frequency-Isolated Power Conversion System for Battery Energy
Authors: Xie Ning , Shu Jie , Chen Jiongcong , Wang Hao , Xie Fei
Published: July 2022

Energy storage technology has become critical for supporting China’s large-scale access to renewable energy. As the interface between the battery energy storage system (BESS) and power grid, the stability of the PCS (power conversion system) plays an essential role. Here, we present a topology of a 10 kV high-voltage energy storage PCS without a power frequency transformer for the establishment of a large-scale energy storage system. We analyzed the energy storage converter’s mechanism and characteristics and also introduced the power-control strategy of the HVAC (high-voltage AC) and LVDC (low-voltage DC) converter module. On this basis, a 10 kV/1 MW high-capacity PCS prototype was designed. Additionally, by simulation and experiment, we proved the correctness of the PCS scheme. The topology and control strategy proposed in this paper can provide cases and technical support for the subsequent promotion and application of new energy and power station energy storage.
Total Frequency Spread: A New Metric to Assess the Switching Frequency Spread of FCS-MPC
Authors: Tricarico Thiago , Costa João Adolpho , Herrera Danilo , Galván-Díez Eduardo , Carrasco Juan M. , Aredes Mauricio
Published: July 2022

This work proposes a metric to assess the spread of the switching frequency profile of power converters, focusing on Finite Control Set-Model-Predictive-Control (FCS-MPC)-based converters, and analyses the effect of the filter sizing, sampling period, and cost function on the switching frequency spread. An algorithm that estimates the instantaneous switching frequency is the basis of the proposed metric. The literature review shows that only two metrics, THD and average switching frequency, are used to assess the switching frequency of FCS-MPC, but the findings of this research show that both are insufficient to characterize FCS-MPC. The main contribution of this paper is that now researchers can quantitatively assess the switching frequency spread of FCS-MPC through the proposed metric.
Clinical Epigenetics on the Baltic Coast
Authors: Maleszka Ryszard
Published: July 2022

This report summarizes the proceedings of the inaugural Clinical Epigenetics Conference that was held in Szczecin, Poland, from 8 June 2022. With focus on epigenetic diseases whose causes, progression, and prognosis are associated with aberrant epigenomic alterations, the meeting was a timely forum to discuss recent progress in this rapidly evolving field and consider avenues for converting experimental data into clinical reality that would be beneficial for patients. The wealth of the presented data was an impressive showcase of the enormous challenges faced by researchers in their quest for understanding the benefits and limitations of the available information in the medical context. A shared view among the participants was that merging the current state of knowledge with clinical applications will be promptly achieved.
Making Mitotic Chromosomes in a Test Tube
Authors: Shintomi Keishi
Published: July 2022

Mitotic chromosome assembly is an essential preparatory step for accurate transmission of the genome during cell division. During the past decades, biochemical approaches have uncovered the molecular basis of mitotic chromosomes. For example, by using cell-free assays of frog egg extracts, the condensin I complex central for the chromosome assembly process was first identified, and its functions have been intensively studied. A list of chromosome-associated proteins has been almost completed, and it is now possible to reconstitute structures resembling mitotic chromosomes with a limited number of purified factors. In this review, I introduce how far we have come in understanding the mechanism of chromosome assembly using cell-free assays and reconstitution assays, and I discuss their potential applications to solve open questions.
Numerical Characterization of the Flow Dynamics and COP Estimation of a Binary Fluid Ejector Ground Source Heat Pump Cooling System
Authors: El Hassan Mouhammad
Published: July 2022

Ejector-based refrigeration systems can make direct use of many forms of thermal energy, such as solar thermal, waste heat, biogas, or natural gas. The present paper describes the estimation of the thermal coefficient of performance (COP) of a binary fluid ejector ground source heat pump (BFE GSHP) cooling system. A method for fluid selection was defined based on the favorable thermo-physical properties of the working fluids. A short list of fluid pairs were selected based on their favorable properties for the BFE GSHP cooling system. Computational Fluid Dynamics (CFD) investigation was conducted for the selected fluid pairs and a suitable ejector geometry is proposed for the high compression ratios encountered in the GSHP applications. The mixing between primary and secondary fluids was investigated using physical analysis of the CFD results. The effect of the fluids’ thermo-physical properties on the system performance was also discussed.
Characterization and Classification of Direct and Commercial Strawberry Beverages Using Absorbance–Transmission and Fluorescence Excitation–Emission Matrix Technique
Authors: Sikorska Ewa , Nowak Przemysław , Pawlak-Lemańska Katarzyna , Sikorski Marek
Published: July 2022

The subject of this study was to characterize the absorption and fluorescence spectra of various types of strawberry beverages and to test the possibility of distinguishing between direct juices and pasteurized commercial products on the basis of their spectral properties. An absorbance and transmission excitation–emission matrix (A-TEEMTM) technique was used for the acquisition of spectra. The obtained spectra were analyzed using chemometric methods. The principal component analysis (PCA) revealed differences in both the absorption spectra and excitation–emission matrices (EEMs) of two groups of juices. The parallel factor analysis (PARAFAC) enabled the extraction and characterization of excitation and emission profiles and the relative contribution of four fluorescent components of juices, which were related to various groups of polyphenols and nonenzymatic browning products. Partial least squares–discriminant analysis (PLS-DA) models enabled 100% correct class assignment using the absorption spectra in the visible region, unfolded EEMs, and set of emission spectra with excitation at wavelengths of 275, 305, and 365 nm. The analysis of variable importance in projection (VIP) suggested that the polyphenols and nonenzymatic browning products may contribute significantly to the differentiation of commercial and direct juices. The results of the research may contribute to the development of fast methods to test the quality and authenticity of direct and processed strawberry juices.
Phenolic Profiling and In-Vitro Bioactivities of Corn (Zea mays L.) Tassel Extracts by Combining Enzyme-Assisted Extraction
Authors: Elsayed Nesren , Marrez Diaa A. , Ali Mohamed A. , El-Maksoud Ahmed Ali Abd , Cheng Weiwei , Abedelmaksoud Tarek Gamal
Published: July 2022

In this work, enzyme-assisted extraction (EAE) of phenolic compounds from corn tassel using cellulase, protease, and their combination (1:1) was developed and optimized by central composite response surface methodology. The phenolic profile of obtained corn tassel extracts (CTE) was elucidated by high-performance liquid chromatography–diode array detection (HPLC–DAD) analysis, and their antioxidative, antimicrobial, and cytotoxic properties were evaluated in vitro. The results showed that CTE by EAE with combined enzymes had the highest total phenolic content (TPC). Under optimum enzymatic conditions, the experimental TPC values were 9.78, 8.45, and 10.70 mg/g, respectively, which were significantly higher than that of the non-enzymatic control (6.75 mg/g) (p < 0.05). Fourteen more phenolic compounds (13.80–1694.36 µg/g) were identified in CTE by EAE with the combined enzymes, and thus the antioxidant activity of that extract, determined by DPPH and ABTS radical scavenging method, was demonstrated to be stronger than that of the extracts by EAE with the single and ethanol extraction. Furthermore, this extract also showed remarkably better antimicrobial properties against all tested food-borne pathogenic bacteria and mycotoxigenic fungi than CTE by other extraction methods. CTE by EAE were nontoxic to normal lung fibroblast cell line (Wi-38) but cytotoxic to human colorectal and lung cancer cell lines (Caco-2 and A549), with IC50 values of 392.62–461.98 and 210.66–359.56 µg/mL, respectively, which indicated its potential anticancer properties. In conclusion, CTE by EAE, especially with the combined use of cellulase and protease, seems to hold promising potential for multifunctional application in food and pharma fields.
Simultaneous Mass Spectrometric Detection of Proteins of Ten Oilseed Species in Meat Products
Authors: Spörl Johannes , Speer Karl , Jira Wolfgang
Published: July 2022

Food fraud is a common issue in the modern food industry. The undeclared use of foreign proteins in meat products is a major concern in this context. Oilseeds are ideal for this purpose due to their high protein content and since huge amounts of oil meal are obtained as a by-product of oil production. Therefore, a UHPLC-MS/MS method was developed for the simultaneous detection of chia, coconut, flaxseed, hemp, peanut, pumpkin, rapeseed, sesame, soy, and sunflower proteins in meat products. Potential tryptic peptide markers were identified by high-resolution mass spectrometry. The final twenty peptide markers selected, which are specific for one of the ten species targeted, were each measured by multiple reaction monitoring. To the best of our knowledge, twelve new heat-stable marker peptides for chia, coconut, flaxseed, pumpkin, rapeseed, sesame and sunflower have not been reported previously. Emulsion-type sausages with 0.01, 0.25, 0.50, 0.75 and 1.00% protein addition by each oilseed species were produced for matrix calibration. No false-positive results were recorded. In the quantification of the ten oilseed species, 466 of 480 measuring data points of the recovery rate in unknown sausages (0.15 and 0.85% protein addition by each oilseed species) were in the accepted range of 80–120%.
Attitudes towards Freshly Made and Readily Prepared Texture-Modified Foods among Speech-Language Therapists, Dietitians, and Community-Dwelling Older Adults
Authors: Wu Xiaojing Sharon , Miles Anna , Braakhuis Andrea
Published: July 2022

Texture-modified foods (TMFs) are recommended for patients suffering from swallowing difficulties. Given the increasing aging population, the use of TMFs is on the rise. Research to date has focused on the nutritional value, malnutrition indices and healthcare practices in relation to TMFs, but the perception of these diets from a patient and healthcare practitioner perspective has received less consideration. This study explored how currently available TMFs (including Soft & Bite-Sized, Minced & Moist, and puree) are perceived by key stakeholders. Four types of TMFs were consumer tested: freshly made TMFs following foodservice recipes and three types of readily prepared TMFs (commercially packaged, sous-vide and hydrolysed). The selected samples were tested through five focus groups (including nine dietitians, seven speech-language therapists, and five community-dwelling older adults), which involved a sensory rating using a validated 7-point scale meal assessment tool and a semi-structured focus group discussion. Analysis was conducted using quantitative and qualitative approaches. Soft & Bite-Sized meals had significantly higher palatability ratings than others. Sous-vide meals were most suitable for Soft & Bite-Sized texture, while commercially packaged samples were most appropriate for minced moist and pureed meals. Three main themes emerged through content analysis: (1) palatability of TMFs, (2) perceived challenges with the currently available TMFs and (3) key differences in opinion between stakeholders. Freshly made TMFs were more appealing and tastier, whereas readily prepared (pre-cooked, packaged and require reheating) TMFs had a more consistent texture. The texture of all TMFs requires enhancement, particularly in pureed meals. Developing nutritious and safe TMFs for people with dysphagia requires the promotion of active insight exchange between dietitians and speech-language therapists.
Clinical and Biological Adaptations in Obese Older Adults Following 12-Weeks of High-Intensity Interval Training or Moderate-Intensity Continuous Training
Authors: Youssef Layale , Granet Jordan , Marcangeli Vincent , Dulac Maude , Hajj-Boutros Guy , Reynaud Olivier , Buckinx Fanny , Gaudreau Pierrette , Morais José A. , Mauriège Pascale , Gouspillou Gilles , Noirez Philippe , Aubertin-Leheudre Mylène
Published: July 2022

Sarcopenia and obesity are considered a double health burden. Therefore, the implementation of effective strategies is needed to improve the quality of life of older obese individuals. The aim of this study was to compare the impact of high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT) on functional capacities, muscle function, body composition and blood biomarkers in obese older adults. Adipose tissue gene expression and markers of muscle mitochondrial content and quality control involved in exercise adaptations were also investigated. Sixty-eight participants performed either HIIT (n = 34) on an elliptical trainer or MICT (n = 34) on a treadmill, three times per week for 12 weeks. HIIT produced significantly higher benefits on some physical parameters (six-minute walking test (HIIT: +12.4% vs. MICT: +5.2%); step test (HIIT: +17.02% vs. MICT: +5.9%); ten-repetition chair test (HIIT: −17.04% vs. MICT: −4.7%)). Although both HIIT and MICT led to an improvement in lower limb power (HIIT: +25.2% vs. MICT: +20.4%), only MICT led to higher improvement in lower limb muscle strength (HIIT: +4.3% vs. MICT: +23.2%). HIIT was more beneficial for increasing total lean body mass (HIIT: +1.58% vs. MICT: −0.81%), while MICT was more effective for decreasing relative gynoid fat mass (HIIT: −1.09% vs. MICT: −4.20%). Regarding adipose tissue gene expression, a significant change was observed for cell death-inducing DFFA (DNA fragmentation factor-alpha)-like effector A (CIDEA) in the HIIT group (A.U; HIIT at T0: 32.10 ± 39.37 vs. HIIT at T12: 48.2 ± 59.2). Mitochondrial transcription factor A (TFAM) content, a marker of mitochondrial biogenesis, increased significantly following HIIT (+36.2%) and MICT (+57.2%). A significant increase was observed in the HIIT group for Translocase of Outer Membrane 20 (TOM20; +54.1%; marker of mitochondrial content), Mitofusin-2 (MFN2; +71.6%; marker of mitochondrial fusion) and Parkin RBR E3 Ubiquitin Protein Ligase (PARKIN; +42.3%; marker of mitophagy). Overall, our results indicate that even though MICT (walking on treadmill) and HIIT (on an elliptical) are effective intervention strategies in obese older adults, HIIT appears to have slightly more beneficial effects. More specifically, HIIT led to higher improvements than MICT on functional capacities, lean mass and skeletal muscle markers of mitochondrial content, fusion, and mitophagy. Thus, MICT but also HIIT (time-efficient training) could be recommended as exercise modalities for obese older adults to maintain or improve mobility, health and quality of life.
Genome-Wide Identification and Expression Pattern Analysis of the TCP Gene Family in Radish (Raphanus sativus L.)
Authors: Mei Yi , Liu Zhe , Zheng Jiaqiu , Wang Weiwei , Zu Yanxia , Wu Yongcheng , Zhang Lina , Feng Ruchao , Shen Feng
Published: July 2022

TCP transcription factors are a unique transcription family in higher plants, and play important roles in plant development, responses to environmental stresses and phytohormones. Radish is an important crop and widely cultivated worldwide. However, genome-wide identification and expression analysis of TCP family in radish has not been reported yet. In this study, 32 RsTCP genes were identified from the whole genome. Phylogenetic analysis showed that the RsTCP family can be divided into two major groups and three subgroups, namely Class I (PCF), Class II (CIN), and Class II (CYC/TB1). Chromosome mapping showed that 32 genes were distributed on all nine chromosomes of radish. Transcriptome data indicated that the transcription of RsTCP genes differed between tissues and developmental stages. Multiple phytohormone-related cis-acting elements in the promoter region of RsTCPs and several post-translational modification sites in RsTCP protein sequences were identified. The real-time quantitative PCR (RT-qPCR) analysis indicated that several RsTCP genes changed significantly in response to the treatments, including low temperature, drought, salt, gibberellin (GA3), and abscisic acid. Among them, RsTCP16 showed significantly higher levels in leaves than in other radish tissues, and the transcription of RsTCP16 was significantly upregulated under the five treatments, especially during low temperature, salt shock, and GA3 response, suggesting that RsTCP16 could be involved in the development of radish leaves and the response to stress. In summary, these results provided a basis for further exploring the molecular mechanism of RsTCP genes in radish.
The SSR Null Allele Problem, and Its Consequences in Pedigree Reconstruction and Population Genetic Studies in Viticulture
Authors: Jahnke Gizella , Smidla József , Deák Tamás , Oláh Róbert , Szőke Barna Árpád , Nyitrainé Sárdy Diána Ágnes
Published: July 2022

Null alleles are alleles that are recessive to codominant markers without any effect on the phenotype. In SSR assays, there are several reasons for the lack of amplification at a locus: the primer does not bind well, longer fragments do not amplify due to imperfections in the PCR reaction, or the amount of DNA in the sample is insufficient. In microsatellite studies, null alleles are mostly used in pedigree analysis and population genetics calculations such as diversity estimation. Null alleles in pedigree analysis can cause rejection of the true parent; if not recognized while in population genetics they distort the results in underestimating diversity. In this review, the effects caused by null-alleles in viticultural research and its possible solutions were summarized.
Cysteamine-Gold Coated Carboxylated Fluorescent Nanoparticle Mediated Point-of-Care Dual-Modality Detection of the H5N1 Pathogenic Virus
Authors: Durairaj Kaliannan , Than Duc Duong , Nguyen Anh Thi Viet , Kim Hak Sung , Yeo Seon-Ju , Park Hyun
Published: July 2022

Globally, point-of-care testing (POCT) is the most preferable on-site technique for disease detection and includes a rapid diagnostic test (RDT) and fluorescent immunochromatographic strip test (FICT). The testing kits are generally insufficient in terms of signal enhancement, which is a major drawback of this approach. Sensitive and timely on-site POCT methods with high signal enhancement are therefore essential for the accurate diagnosis of infectious diseases. Herein, we prepare cysteamine-gold coated carboxylated europium chelated nanoparticle (Cys Au-EuNPs)-mediated POCT for the detection of the H5N1 avian influenza virus (AIV). Commercial nanoparticles were used for comparison. The spectral characteristics, surface morphologies, functional groups, surface charge and stability of the Cys AuNPs, EuNPs, and Cys Au-EuNPs were confirmed by UV-visible spectrophotometry, fluorescence spectrometry, transmission electron microscope with Selected area electron diffraction (TEM-SAED), Fourier-transform infrared spectroscopy (FTIR) and zeta potential analysis. The particle size distribution revealed an average size of ~130 ± 0.66 nm for the Cys Au-EuNPs. The Cys Au-EuNP-mediated RDT (colorimetric analysis) and FICT kit revealed a limit of detection (LOD) of 10 HAU/mL and 2.5 HAU/mL, respectively, for H5N1 under different titer conditions. The obtained LOD is eight-fold that of commercial nanoparticle conjugates. The photo luminance (PL) stability of ~3% the Cys Au-EuNPs conjugates that was obtained under UV light irradiation differs considerably from that of the commercial nanoparticle conjugates. Overall, the developed Cys Au-EuNPs-mediated dual-mode POCT kit can be used as an effective nanocomposite for the development of on-site monitoring systems for infectious disease surveillance.
Nuclear Transport of Respiratory Syncytial Virus Matrix Protein Is Regulated by Dual Phosphorylation Sites
Authors: Ghildyal Reena , Teng Michael N. , Tran Kim C. , Mills John , Casarotto Marco G. , Bardin Philip G. , Jans David A.
Published: July 2022

Respiratory syncytial virus (RSV) is a major cause of respiratory infections in infants and the elderly. Although the RSV matrix (M) protein has key roles in the nucleus early in infection, and in the cytoplasm later, the molecular basis of switching between the nuclear and cytoplasmic compartments is not known. Here, we show that protein kinase CK2 can regulate M nucleocytoplasmic distribution, whereby inhibition of CK2 using the specific inhibitor 4,5,6,7-tetrabromobenzo-triazole (TBB) increases M nuclear accumulation in infected cells as well as when ectopically expressed in transfected cells. We use truncation/mutagenic analysis for the first time to show that serine (S) 95 and threonine (T) 205 are key CK2 sites that regulate M nuclear localization. Dual alanine (A)-substitution to prevent phosphorylation abolished TBB- enhancement of nuclear accumulation, while aspartic acid (D) substitution to mimic phosphorylation at S95 increased nuclear accumulation. D95 also induced cytoplasmic aggregate formation, implying that a negative charge at S95 may modulate M oligomerization. A95/205 substitution in recombinant RSV resulted in reduced virus production compared with wild type, with D95/205 substitution resulting in an even greater level of attenuation. Our data support a model where unphosphorylated M is imported into the nucleus, followed by phosphorylation of T205 and S95 later in infection to facilitate nuclear export and cytoplasmic retention of M, respectively, as well as oligomerization/virus budding. In the absence of widely available, efficacious treatments to protect against RSV, the results raise the possibility of antiviral strategies targeted at CK2.
Potential of the Stromal Matricellular Protein Periostin as a Biomarker to Improve Risk Assessment in Prostate Cancer
Authors: Doldi Valentina , Lecchi Mara , Ljevar Silva , Colecchia Maurizio , Campi Elisa , Centonze Giovanni , Marenghi Cristina , Rancati Tiziana , Miceli Rosalba , Verderio Paolo , Valdagni Riccardo , Gandellini Paolo , Zaffaroni Nadia
Published: July 2022

Prostate cancer (PCa) ranges from indolent to aggressive tumors that may rapidly progress and metastasize. The switch to aggressive PCa is fostered by reactive stroma infiltrating tumor foci. Therefore, reactive stroma-based biomarkers may potentially improve the early detection of aggressive PCa, ameliorating disease classification. Gene expression profiles of PCa reactive fibroblasts highlighted the up-regulation of genes related to stroma deposition, including periostin and sparc. Here, the potential of periostin as a stromal biomarker has been investigated on PCa prostatectomies by immunohistochemistry. Moreover, circulating levels of periostin and sparc have been assessed in a low-risk PCa patient cohort enrolled in active surveillance (AS) by ELISA. We found that periostin is mainly expressed in the peritumoral stroma of prostatectomies, and its stromal expression correlates with PCa grade and aggressive disease features, such as the cribriform growth. Moreover, stromal periostin staining is associated with a shorter biochemical recurrence-free survival of PCa patients. Interestingly, the integration of periostin and sparc circulating levels into a model based on standard clinico-pathological variables improves its performance in predicting disease reclassification of AS patients. In this study, we provide the first evidence that circulating molecular biomarkers of PCa stroma may refine risk assessment and predict the reclassification of AS patients.
A Preparative Method for the Isolation of Calponin from Molluscan Catch Muscle
Authors: Lazarev Stanislav S. , Shevchenko Ulyana V. , Dyachuk Vyacheslav A. , Vyatchin Ilya G.
Published: July 2022

We describe the development of a preparative method to isolate molluscan catch muscle, calponin. This method is based on the ability of calponin to interact with actin in a temperature-dependent manner. After extracting thin filaments, as previously described, the extract was ultracentrifuged at 2 °C. While other surface proteins of thin filaments co-precipitated with actin, calponin, along with some minor contaminants, remained in the supernatant. Calponin was purified through cation-exchange chromatography. The yield of pure protein was four-fold higher than that achieved through high-temperature extraction. To evaluate functionally isolated proteins, we determined the effect of calponin on Mg2+-ATPase activity of hybrid and non-hybrid actomyosin. The degree of ATPase inhibition was consistent with previously published data but strongly dependent on the environmental conditions and source of actin and myosin used. Furthermore, at low concentrations, calponin could induce the ATPase activity of hybrid actomyosin. This result was consistent with data indicating that calponin can modulate actin conformation to increase the relative content of “switched on” actin monomers in thin filaments. We assume that calponin obtained by the isolation method proposed herein is a fully functional protein that can both inhibit and induce the ATPase activity.