bluuebunny/crossref_metadata_2025 huggingface dataset:
Safety and specific activity of the recombinant SARS-CoV-2 allergen (“CoronaDerm-PS”) based on phase I–II clinical trial results
Authors: Savin Tikhon V. , Milichkina A. M. , Krasnov A. A. , Kuznetsova R. N. , Shchederkina E. E. , Svarval A. V. , Sharova A. А. , Reingardt D. E. , Ostankova Yu. V. , Gubanova A. V. , Petrova O. A. , Zhimbaeva O. B. , Razumovskaya A. P. , Drozd I. V. , Rubinstein A. А. , Trulev A. S. , Kudryavtsev I. V. , Riabchenkova A. A. , Chirak E. L. , Chirak E. R. , Saenko A. I. , Kopat V. V. , Dukhovlinov I. V. , Simbirtsev A. S. , Totolian A. A.
Published: December 2024

The evaluation of cellular immunity to SARS-CoV-2 is a critical tool for assessing vaccination efficacy and development of post-infectious immunity. According to available studies, virus-specific T cells persist longer than antigen-specific antibodies and play a pivotal role in the effective virus elimination from human body. Current methodologies for assessing virus-specific T cells are mostly based on flow cytometry, which require specialized laboratory equipment and highly trained personnel. An alternative approach involves a skin test conducted to assess delayed-type hypersensitivity (DTH) reactions. For this, there has been developed the “CoronaDerm-PS” substance, a sterile isotonic solution for intradermal administration, containing a recombinant hybrid protein that incorporates regions of SARS-CoV-2 structural proteins S, M, N, and E, produced by a genetically modified E. coli BL21 strain cell culture. Following preclinical studies that demonstrated the safety of the above-noted medication, Phase I and Phase II clinical trials were initiated to evaluate safety and specific activity of “CoronaDerm-PS” in apparently healthy volunteers with SARS-CoV-2 post-vaccination and post-infection immunity. In Phase I clinical trial with COVID-19 unexposed or vaccinated volunteers, the data were obtained demonstrating the safety and good tolerability of the medication, thus enabling the progression to Phase II. The Phase II results provided additional evidence on the preparation’s safety in individuals with SARS-CoV-2 post-vaccination and post-infection immunity, as assessed by clinical and laboratory data. No serious adverse events were observed during the study, and in 93.5% cases, adverse events required no therapeutic or diagnostic intervention. To assess the specific activity of the preparation, the skin test data were compared with the IFNγ production stimulation index for CD4+ T lymphocytes assessed by flow cytometry. The ROC analysis revealed sensitivity magnitude ranging from 76.6% to 84%, and specificity level ranging from 80% to 87.5%. Based on ROC analysis results, “CoronaDerm-PS” can be an informative diagnostic tool (AUC = 0.795), demonstrating high sensitivity (79.8%) and specificity (80.8%), with consistent results across different volunteer cohorts. Analyzing the collected data suggests that “CoronaDerm-PS” is a robust alternative to laboratory methods for evaluating SARS-CoV-2-specific T-cell immunity, with high sensitivity and specificity, suitable for large-scale screening.
Structured tb risk factors in regions with low and high population density
Authors: Zagdyn Zinaida M. , Zudin A. B. , Kobesov N. V. , Vasileva T. P. , Galoyan A. S. , Verbitskaya E. V.
Published: December 2024

In Russia, socio-demographic, health care management, climatic and environmental TB risk factors such as population density, intensity of social contacts, availability of medical care, seasonal climate changes, environmental issues affecting quality of public health have been holding the leading place while a life standard elevates, and epidemic situation becomes improved. However, the magnitude of specific population density-related TB risk factors in the Russian regions has not been examined yet. The study aim was to assess structured TB risk factors in the Russian regions with high and low population densities decreasing public health quality. Materials and methods. There was conducted a study using sociological, cluster-quota approaches, with respondent-based sample and a face-to-face distribution questionnaire provided by 2500 subjects, of which 1497 were from the North Ossetia-Alania Republic with a high population density, 1003 — from the Republic of Karelia with a low population density. A significance of differences was assessed by using exact criterion Fisher using a contingency table. Results. In the Republic of Karelia, compared with North Ossetia-Alania Republic, despite a higher gross regional product (527.8 thousand rubles vs 293.4 thousand rubles, p 0.000), social and behavioral TB risk factors were significantly elevated: fewer respondents with high education (18.4% vs 33.6%, p 0.000), higher divorced (18.7% vs 26.9%, p 0.022), higher number of those living in apartment buildings (65.0% vs 39.5%, p 0.000), more hired and seasonal workers, unemployed, pensioners due to age and disability (55.0% vs 32.3%, p 0.000), more alcohol abusers (67.4% vs 34.3%, p 0.000), “heavy” smokers (20.8% vs 7.2%, p 0.000), more subjects with chronic diseases (43.7% vs 32.1%, p 0.02); more issues related to accessibility to medical care: municipal policlinics are less available (42.7% vs 85.6%, p 0.000), the distant location of medical facilities (21.4% vs 2.7%, p 0.000), lack of transport links (15.7% vs 8.5%, p 0.000), poor roads (5.9% vs 16.9%, p 0.000) and few medical specialists (60.9% vs 16,8%, p 0.000) are more often noted. In the North Ossetia-Alania Republic vs the Republic of Karelia, respondents had more frequent social contact with TB patients (66.9% vs 34.8%, p 0.000). Conclusion. The epidemiological TB factors in the Russian regions with high population density hold a lead place; in regions with low population density, social, behavioral and accessibility to medical care factors remain relevant, which should be taken into account while developing measures for efficient management of epidemic process.
The frequency of detected pathogen genomes in children with acute respiratory infections
Authors: Tyukavkina S. Yu. , Kharseeva Galina G. , Kostinov M. P. , Alieva A. A. , Balahnova V. V. , Alutina E. L. , Chaikina V. A. , Volkova V. V.
Published: December 2024

Due to the high prevalence of acute respiratory infections in children, it was of interest to determine the composition of bacterial and viral-bacterial associations for respiratory tract inhabiting microorganisms. To identify respiratory tract pathogens, especially viruses and unculturable bacteria, it is advisable to use molecular genetic research methods particularly PCR. The work was aimed at comparatively analyzing rate of genome detection for respiratory tract pathogens in children with acute respiratory infections and apparently healthy controls. Materials and methods. Children (97 people) were examined, of which 35 people — with acute respiratory infections of the upper respiratory tract (nasopharyngitis, pharyngitis, laryngitis) (ARI URT), 32 people — with acute respiratory infections of the lower respiratory tract (acute bronchitis, acute obstructive bronchitis) (ARI NDP) and 30 people — apparently healthy control at the time of examination. RNA and DNA of viral and bacterial pathogens were assessed in the oropharyngeal smears using PCR. Test systems produced by the Federal Budgetary Institution Central Research Institute of Epidemiology of Rospotrebnadzor and JSC Vector-Best were used. Results. An analysis of detection rate for DNA and RNA of bacterial and viral pathogens showed the presence of a wide range of them (20 different species/strains out of 27 identified) in oropharyngeal biomaterial from all examined children. The peak diversity and detection rate of pathogen genomes were found in apparently healthy subjects and children with ARI UDP (DNA of members from Herpesviridae family, S. pneumoniae, H. influaenzae, MSSA, MRCoNS). Associations of 4–6 pathogens prevailed in the microflora, and the “core” of both bacterial and viral-bacterial associations was the combination of S. pneumoniae and H. influenzae (in 93.3% and 60.0% of cases, respectively). In bacterial associations, staphylococci (MRSA and MRCoNS) and P. aeruginosa were also detected in healthy children, MSSA and other very rarely bacterial types were detected in children with ARI UDP. In children with NPD infections, the majority of whom (75.0±7.7%) were classified as long-term ill and had a complicated disease course, a noticeable depletion (decrease in taxonomic diversity) of the nasopharyngeal microbiota was found. Conclusion. The presented data indicate the presence in the biomaterial of the examined children of molecular genetic markers of a wide range of pathogens presented as viral-bacterial and bacterial associations, with varying composition being related to patient clinical status. Detection of pathogen genomes is important for choosing proper etiotropic therapy at early disease stages as well as prescribing probiotic drugs that can restore a balance in respiratory tract microflora.
Total immunoglobulin E as a zooanthroponosis predictor in healthy volunteers: an observational study
Authors: Zamyatina Evgenia V. , Shevela A. I. , Mandel I. A. , Sizov A. A. , Stepanov A. A. , Sizov D. A. , Danilenko S. O.
Published: December 2024

Control of zoonotic infections common in animals and humans profoundly contributes to maintaining public health. The source of pathogens for humans is primarily presented by those animals with which they frequently come into contact during agricultural work, hunting, and gathering mushrooms or wild berries as well as in domestic settings (dogs, cats, other domestic animals, rodents). The study was conducted by the Center of New Medical Technologies, Novosibirsk, and involved 111 healthy volunteers. The specific immune response against the following zoonoses was analyzed: toxocariasis, toxoplasmosis, brucellosis, listeriosis, leptospirosis, and opisthorchiasis, based on specific IgG antibodies. Specific parasitic immune response was compared with the clinical data of the participants and blood IgE levels. Thirty-nine (35%) out of 111 participants had anti-parasitic IgG antibodies against the following zoonoses: leptospirosis, listeriosis, toxocariasis, toxoplasmosis, and opisthorchiasis. Among these 39 participants, 11 had antibodies specific to two or more zoonoses comprising 10% of the cohort. The IgE vs IgG level in the presence of any zoonosis significantly differed (p = 0.0001). Forty-seven participants had normal IgE levels, of whom 8 (17%) had IgG antibodies specific to one of the studied zoonoses. Sixty-four participants had elevated IgE levels, of whom 31 (48%) had IgG antibodies specific to one or more zoonoses, p = 0.001. The IgE level may be a predictor of the zoonoses in apparently healthy individuals at IgE level 0.226 IU (area under the curve 0.73 [0.62–0.83], p = 0.0001; sensitivity 76%, specificity 62%). The identified diagnostic criteria for screening zoonoses can accelerate the differential diagnosis for conditions associated with non-specific complaints. Prospective randomized studies are needed to identify early pathology-related diagnostic criteria.
THE IMPACT OF CONCOMITANT DISEASES DURING SARS-COV-2 INFECTION IN RESIDENTS OF KAZAKHSTAN
Authors: Ashirova Mayra Zangarovna , Abuova Gulzhan Narkenovna , Seytkhanova Bibigul  Toelegenovna
Published: December 2024

Background . Since the onset of the COVID -19 pandemic, interindividual variability in the course of the disease has been reported , indicating a wide range of factors influencing it. Factors most commonly associated with increased severity of COVID -19 disease include older age, obesity, and diabetes. The impact of the cytokine storm is complex, reflecting the complexity of the immunological processes triggered by SARS - CoV -2 infection. A modern problem such as a worldwide pandemic requires modern solutions, which in this case involve the use of machine learning to analyze differences in the clinical properties of populations affected by the disease and then assess its significance, which in turn leads to the creation of a tool that applicable to assess the individual risk of infection with SARS - CoV -2.
Methods . Values of biochemical and morphological parameters of 781 patients ( Kazakhstan, Shymkent ) were collected and used for static analysis. The Spearman rank correlation coefficient formula was used to estimate the correlations between each of the traits in the population .
Results . The highest correlation coefficients were shown by such parameters as , patient's age and sex , serum glucose, while the highest inverse correlation coefficient was estimated for serum red blood cell count.
Conclusion . The current analysis indicates a range of parameters available for routine screening in clinical settings. A tool based on these parameters is also presented, useful for assessing the individual risk of developing COVID -19 in patients. A limitation of the study is the demographics of the study population, which may limit its general applicability.
Taxpayer Satisfaction Transformation: A Text Mining Analysis of Service Delivery at Jakarta’s Tax Offices
Authors:   Universitas Terbuka, South Tangerang, Indonesia, Thaha Abdurrahman Rahim , Purnamasari Rita ,   Universitas Telkom, Bandung, Indonesia, Antoro Aji Fajar Suryo ,   Universitas Terbuka, South Tangerang, Indonesia
Published: December 2024

Taxpayer satisfaction is critical to driving voluntary compliance and the overall effectiveness of the tax system. In Jakarta, service quality at the Tax Office (KPP) significantly affects taxpayer perceptions and satisfaction. Positive interactions with KPP foster trust in government institutions and increase voluntary tax compliance. This study uses advanced data analysis techniques to assess taxpayer satisfaction by analyzing more than 8,000 Google reviews of tax offices in Jakarta. Sentiment analysis categorizes reviews into positive, neutral and negative sentiments. Topic modeling with Latent Dirichlet Allocation (LDA) identified key themes, such as service efficiency, administrative procedures, and staff interaction. K-means clustering grouped the tax offices based on service perceptions. This analysis shows significant variation in taxpayer satisfaction across different tax offices. Some tax offices excel in terms of service efficiency and clarity, receiving mostly positive reviews, while others struggle with suboptimal service delivery and lengthy processes, resulting in negative feedback. Topic modeling highlighted key areas of concern for taxpayers, including service speed, administrative procedures, and staff interaction. Clustering analysis identified distinct groups of tax offices, each with unique challenges and strengths in service delivery. The findings suggest targeted interventions to address specific issues in underperforming tax offices. By aligning service improvements with issues identified by taxpayers, tax authorities can significantly improve satisfaction and compliance. This approach allows for strategies to be tailored to the specific needs of each cluster. This study contributes to public sector reform by showing how taxpayer feedback can be systematically analyzed to inform service improvements. The methodology and findings offer a replicable model for other regions looking to use data-driven insights to improve government services. Improving taxpayer satisfaction is critical to fostering greater public trust and achieving higher tax compliance.
Review of the development of various primary health care aspects in the context of national health systems of the member states of the Commonwealth of Independent States. Part 1: Organizational aspect
Authors: Drapkina O. M. , Shepel R. N. , Korotkova A. V. , Naumova Yu. S. , Hagverdiyev G. O. , Shcherbinsky A. A. , Sachek M. M. , Kulkaeva G. U. , Brimkulov N. N. , Muhsinzoda G. M. , Uralieva I. R. , Ogneva E. Yu.
Published: December 2024

The article based on an analytical review of information sources on the status and development of various aspects of primary health care (PHC) in the world and in selected countries, as well as international evidencebased recommendations, to assess the prospects of using international experience and recommendations for the development of PHC in the Commonwealth of Independent States (CIS) member states with regard to the organizational aspect, which is considered in this article.

A database of scientific publications, regulatory legal documents, and Internet resources was used with an emphasis on evidence-based reviews and methodological documents that were included on the basis of selected keywords and concepts related to organization aspect of health systems and PHC and characteristics, as well as by the criteria of potential impact on the results of PHC provision. Priority in the search was given to materials from the last decade and to the countries with similar models of health systems.

The characteristics of the main aspects of national PHC subsystems — organizational, management, resources, financing, digitalization and results — were identified, systematized and ordered in the structure of the PHC characteristics matrices in the selected countries.

This two-part article presents results of an analysis of the development of the organizational aspect of PHC and its characteristics, including the implementation of recommendations selected in the review process with proven effectiveness in the CIS member states.

Analysis of the development since 2002 of the WHO idea of the transition of the PHC subsystem organization from the "radar" principle associated with the concept of "completed case of medical care" to the continuous and permanent management of patients with chronic diseases by a team of specialists, as well as the experience of the CIS member states in the medical examination of the attached population and dispensary supervision (implementation of management programs) for patients with chronic diseases gave reason to propose an updated name for the principle of constant medical care in managing people health throughout their lives — the principle of "permanence". And also suggest using it not only in the management of chronic conditions,
Screening of malignant neoplasms of the breast as part of the medical examination of certain groups of the adult population. Guidelines
Authors: Drapkina O. M. , Kaprin A. D. , Almazova I. I. , Drozdova L. Yu. , Ivanova E. S. , Rozhkova N. I. , Rubtsova N. A. , Khailova J. V. , Shepel R. N.
Published: December 2024

The Guidelines contain information on the theoretical foundations of screening for malignant neoplasms of the breast, regulatory legal acts regulating the organization of screening activities within the framework of medical examination of certain groups of the adult population, diagnostic algorithms and routing of the examined persons. Guidelines have been developed for the heads of medical organizations providing primary health care in terms of organizing processes, internists, general practitioners, medical workers from among the secondary medical personnel who are directly involved in the implementation of the process of medical examination of certain groups of the adult population.
Influence of the structure component on the quality of Primary Health Care in the Federal District
Authors: Scherer Magda Duarte dos Anjos , Forte Elaine , Abreu Brenda Ferreira de , Aguiar Ricardo Saraiva , Santos Elizel Monteiro dos , Xavier Mayra Fernandes , Poças Katia Crestine
Published: December 2024

ABSTRACT The aim of this descriptive qualitative study was to analyze the influence of the structure component of health services on the quality of Primary Health Care (PHC) in the Federal District in the perception of local managers, within the scope of the Primary Care Qualification Program. The data was produced in Focus Groups with 77 managers of Basic Health Units (UBS) in 2020. The content was analyzed using the Idea Association Map and coded in the ATLAS.ti® software in the light of the Thematic Content Analysis. The inadequate number of professionals in the teams, especially community health agents, the non-standardization of work processes, the non-definition of coverage areas, the lack of equipment maintenance, information systems that do not communicate, the absence of vehicles and the precarious structure of some UBS were the main findings that represent structural requirements that influence the quality of PHC. The contribution of this study to guide strategies for improve the structure of services is highlighted, an essential step for the provision of safer and quality care in PHC in the Federal District.
Formative experience using the electronic portfolio as a teaching-learning methodology
Authors: Silva Taís Gabriele Alves da , Almeida Isabella Silva de , Sousa Denise Lins de
Published: December 2024

ABSTRACT The Primary Care Qualification Program aims to strengthen Primary Health Care in the Federal District by developing proactive health actions from the perspective of workers, managers and representatives of users of the Unified Health System. In 2022, the Specialization Course in Family Health Management began, using the electronic portfolio (e-Portfolio) as a proactive, reflective and dialogical methodology. Within each module, interventions were proposed to deal with everyday challenges. The construction of the e-Portfolio resulted in six interventions focused on the management of the Family Health Strategy and aligned with the qualification of user care. The use of this technological resource generated positive results not only for management, but also for staff, users and the community. In addition, it facilitated the feedback process in the training journey, with the possibility of shared construction, in which the teacher-tutor accompanies the writing, the reflections, engages with supporting theoretical content, enhancing the learning process and changes in daily practices.
Use of e-SUS reports for Primary Health Care in the work routine of Family Health teams
Authors: Passos Taciana Silveira , Alencar Tiago Machado de , Brito Alessandra Page , Sena Ayrton Martins Pereira , Santos Débora Bleza , Barreto Agilran Araújo , Silva Lídia Glasielle de Oliveira
Published: December 2024

ABSTRACT The objective was to analyze the use of e-SUS Primary Health Care reports in the work routine of Family Health teams (eSF) in the Federal District (DF). This is a descriptive study of data from the on-site Assessment and Quality Action Plan of the 1st evaluation cycle of the Primary Health Care Qualification Program, between 2021 and 2022. All 607 teams were included. Associations between Health Regions were identified using Chi-square tests, p-value < 0.05. To quantify problems and actions, Data Mining techniques were used. The majority of eSFs in the DF denied using any e-SUS APS report in their work routine (53.23%). The reports that the eSF used most were production reports (54.43%), and management reports the least (31.60%). The regions with the highest proportion of use were Central-South (73.67%), and with the lowest, Southwest (34.56%), p < 0.0001. Some of the main problems described were intrinsic to e-SUS APS (errors, failures, system instability and lack of unification) and others were related to extrinsic issues (lack of knowledge, inconsistency and lack of registration). The teams listed in their Action Plans, as a priority, the training for correct use of the information system.
Equipes de Saúde da Família no Distrito Federal: um olhar geoespacial para as iniquidades
Authors: Santos Elizel Monteiro dos , Teixeira Renata Pella , Batista Sandro Rogério Rodrigues , Lima Mirlene Guedes de , Santos Wallace Enrico Boaventura Gonçalves dos
Published: December 2024

RESUMO A Estratégia Saúde da Família é reconhecida como capaz de minimizar os efeitos das iniquidades em saúde. Este estudo descritivo buscou apresentar a distribuição das equipes de Saúde da Família (eSF) e Unidades Básicas de Saúde (UBS) no Distrito Federal (DF), usando como parâmetros o Índice de Vulnerabilidade da Saúde (IVSaúde) e a densidade demográfica categorizadas por setores censitários. Foram utilizados dados do último censo demográfico publicado e dados oficiais do Cadastro Nacional de Estabelecimentos de Saúde e da Secretaria de Estado de Saúde do DF. Os resultados mostraram que o DF tem uma população heterogênea, quando se trata de vulnerabilidade, e que a maioria dos setores censitários de alto risco está nas zonas periféricas. O DF possui 165 UBS e 615 eSF (média: 3,7 equipes/ UBS). Cerca de 35% dos setores censitários apresentam IVSaúde de baixo risco, e 19,8%, de elevado e muito elevado risco. O estudo mostrou que a maior parte das UBS e equipes está localizada em lugares de alta densidade demográfica e de maior vulnerabilidade, embora ainda seja necessário aumentar o número de equipes e de UBS para uma adequada cobertura do modelo adotado na capital federal, bem como reduzir a razão de equipes por estabelecimento de saúde.
Institucionalización de la evaluación y monitoreo de la Atención Primaria de Salud en el Sistema Único de Salud (SUS): contribuciones para una agenda estratégica de investigación
Authors: Vilasbôas Ana Luiza Queiroz , Nedel Fúlvio Borges , Aquino Rosana , Batista Sandro Rogério Rodrigues , Almeida Ana Paula Santana Coelho , Cury Geraldo Cunha , Mendonça Maria Helena Magalhães de , Sarti Thiago Dias , Facchini Luiz Augusto , Giovanella Lígia
Published: December 2024

RESUMEN La Atención Primaria de Salud es la base del Sistema Único de Salud (SUS). Fortalecerla exige una permanente evaluación de sus procesos y resultados. La Red de Investigación en APS (Rede APS) promueve un intenso debate sobre el fortalecimiento del modelo de APS de base territorial, comunitaria y como ordenadora de la Red de Atención en Salud en el Sistema Único de Salud (SUS). El objetivo de este ensayo es presentar un panorama histórico de las iniciativas de monitoreo y evaluación de la APS brasileña y proponer una agenda de investigación comprometida con la consolidación del SUS y con el fortalecimiento de la Estrategia Salud de la Familia (ESF). Esto como resultado de la reflexión crítica acerca de la producción de conocimiento sobre APS en Brasil, fruto del debate en relación con la agenda política y estratégica de la Rede APS con la participación de actores sectoriales. La agenda está construida en ocho ejes centrales, teniendo en cuenta el modelo de APS integrado en las redes de atención, la gestión y financiamiento, la gestión del trabajo, la salud digital, la infraestructura, la atención integral en salud y el monitoreo y evaluación. La Rede APS defiende la universalización de la cobertura por una ESF cualificada y la construcción de una Política Nacional de Monitoreo y Evaluación para orientar la gestión y el cuidado en la APS en el SUS.
FSC-STD-BRA-01-2025: MUDANÇAS E ATUALIZAÇÕES NA NORMA DE PLANTAÇÕES FLORESTAIS FSC
Authors: Machado Gabriela , Balbinot Rafaelo
Published: December 2024

Conhecido pela promoção do bom manejo florestal, o sistema de certificação Forest Stewardship Council (FSC) atua desde 1993 no setor florestal por meio do manejo florestal e da cadeia de custódia garantindo a rastreabilidade e origem dos produtos e matérias-primas. Seu funcionamento se dá através do atendimento de princípios, critérios e indicadores de normas aplicadas ao escopo da organização e avaliado por uma terceira parte independente. No manejo florestal as normas se dividem em plantadas, naturais e pequenos produtores e de tempos em tempos passam por atualizações para manter o alinhamento às necessidades da sociedade. Nesse sentido, as normas de manejo passam por atualizações desde o ano de 2015. O processo de atualização da norma para plantações florestais foi concluído em setembro de 2024 com a publicação da nova versão. A partir da sua publicação todas as organizações certificadas ou em vias de certificar, deverão se adequar e passar por auditoria com base nos novos indicadores. Nesse sentido, objetiva-se com este estudo realizar uma análise comparativa das normas FSC-STD-BRA-01 2014 V1-1 e FSC-STD-BRA-01-2025. A partir desta, concluiu-se que os princípios 3, 5, 7, 8 e 9 não sofreram muitas alterações, mantendo as temáticas previamente abordados no STD-2014. Por outro lado, os critérios e indicadores dos princípios 1, 2, 4, 6 e 10 foram reorganizados por meio da distribuição para novos princípios e criação de novos critérios e indicadores. Além disso, um ponto importante foi a inclusão de indicadores específicos para SLIMF’s, juntamente com a inclusão dos temas conversão e remediação, CLPI, ações anticorrupção, indicadores para igualdade e equidade de gênero e inclusão de indicadores para produtos não-madeireiros.
Desporto, política social e pobreza em Portugal: análise do financiamento do Programa Nacional Desporto para Todos (PNDpT)
Authors: Barbosa Passos Felipe , Fernando Avalone Athayde Pedro
Published: December 2024

Esta investigação buscou examinar a configuração do financiamento e do gasto público das políticas desportivas destinadas à generalização da prática desportiva em Portugal, em especial aquelas que preveem vagas a população pobre do país. Os dados vinculados ao Programa Nacional de Desporto para Todos (PNDpT) foram nossa base empírica de estudo, os quais demonstraram que o Estado português participa de maneira tímida do orçamento do IPDJ, sendo as apostas esportivas e online a maior fonte de financiamento deste órgão. Sobre o PNDpT apontamos que a fatia orçamentária média do IPDJ em 2022 se limitou a 13% dos custos totais, o que tende a dificultar a expansão deste programa. Como reflexão, sugerimos que os recursos poderiam ser mais bem partilhados, pois enquanto o PNDpT consome a quantia módica de 2,5% do orçamento do IPDJ, as transferências para as federações desportivas avançam em 75%.
La transición alimentaria y nutricional de la infancia en Ecuador: un enfoque pluralista y pragmático
Authors: Rivera Jairo
Published: December 2024

La transición alimentaria y nutricional a nivel global se enmarca en la coexistencia de la desnutrición infantil y el sobrepeso infantil y cada país y localidad transita un nivel propio de prevalencias. El objetivo del presente artículo es analizar la transición alimentaria y nutricional de la infancia en Ecuador a través de un enfoque pluralista y pragmático asociado con las “ciudades” de Boltanski y Thévenot (2006). Para ello, se emplea una metodología mixta basada, por un lado, en un enfoque cuantitativo que se apoya en las bases de datos de las encuestas nacionales de nutrición infantil y, por otro, en un enfoque cualitativo mediante la información primaria recopilada durante el trabajo de campo en las provincias con mayores prevalencias de desnutrición en el país. Entre los principales resultados se encuentra que la transición alimentaria y nutricional se asocia con cambios en los hábitos de consumo, con la limitada práctica de la lactancia materna y con la persistencia de barreras estructurales como la pobreza.
Antropoceno y acción política: la emergencia de una nueva era barroca
Authors: Roth Deubel André-Noël
Published: December 2024

El Antropoceno, en cuanto hecho geológico y biológico, constituye un momento barroco en la medida en que es un encuentro de resultado incierto entre dos verdades irreconciliables. La verdad hasta ahora considerada como tal, la verdad clásica, se ve desafiada por otra verdad, aunque esta última no pueda imponerse. La conciencia de este enfrentamiento, irreconciliable por el momento, incita al género humano a una acción política espectacular y retórica, es decir, barroca.
Adaptación de la planificación pública a las políticas de transición energética y del uso sostenible del suelo en Francia
Authors: Nessi Hélène
Published: December 2024

En el presente artículo se cuestiona la producción de proyectos de ordenación pública en relación con las órdenes ecológicas dirigidas a la sobriedad de la tierra, de la energía y a la descarbonización. Estas políticas de transición europeas imponen un verdadero viraje para la planificación en Francia. El aumento en potencia de los desafíos energéticos y por lo tanto técnicos, y la obligación de un enfoque de la tierra literalmente trastocada, interrogan al planificador sobre la cuestión del perímetro de legitimidad de los instrumentos territoriales, la necesidad de contar con una sólida competencia en ingeniería urbana y sobre el futuro del proyecto urbano como tal.
Análisis de Buenas Prácticas Internacionales en Gobernanza y Políticas Públicas relacionadas con los Servicios Públicos
Authors: Pessina María
Published: December 2024

Este artículo analiza algunas de las mejores prácticas internacionales en gobernanza y políticas públicas relacionadas con los servicios públicos, basándose en las discusiones y hallazgos del seminario "Transformando los Servicios Públicos". Se exploran temas como la descentralización, la cohesión territorial, la regulación de plataformas tecnológicas y la participación ciudadana, todos ellos fundamentales para mejorar la eficiencia y equidad en la provisión de servicios. Se argumenta que la adopción de tecnologías emergentes, como la inteligencia artificial y el Internet de las Cosas, y la implementación de políticas de gobernanza inclusivas son esenciales para gestionar los desafíos del siglo XXI. Además, se propone que la participación ciudadana debe ser el eje central de la gestión pública para asegurar la transparencia y legitimidad de las decisiones. Las recomendaciones presentadas proporcionan una hoja de ruta para avanzar hacia una gestión de servicios públicos más equitativa y eficiente, garantizando el acceso universal a servicios de calidad.
Microsomal stability assay for human and mouse liver microsomes - drug metabolism v1
Authors: Bortnichuk Lina , Pashchenko Anna , Kheilik Yurii
Published: December 2024

Microsomal stability is a key parameter in drug discovery and development to support understanding of a compound's susceptibility to liver metabolism. This assay assesses a compound's susceptibility to metabolism by liver enzymes, primarily cytochrome P450 (CYP) enzymes. Using either human or mouse microsomes. The data analysis provides elimination constant, half-life, and intrinsic clearance . These are determined as results using linear regression analysis. In this protocol, we measure (human or mouse) liver microsomal stability assay for 12 test articles in 96-well plate format. Microsomal incubations are carried out in 5 aliquots of 30 μL (one for each time point). The liver microsomal incubation medium comprises phosphate buffer (100 mM, pH 7.4), MgCl2 (3.3 mM), NADPН (3 mM), glucose-6-phosphate (5.3 mM), glucose-6-phosphate dehydrogenase (0.67 units/mL) with 0.415 mg of liver microsomal protein per mL. In the negative control reactions, the NADPH-cofactor system is substituted with phosphate buffer. Test compounds (2 μM, final acetonitrile concentration 1.6 %) are incubated with microsomes at 37°C, shaking at 100 rpm. Five time points over 40 minutes are analyzed (0, 7, 15, 25, and 40 min). The reactions are stopped by adding 5 volumes of acetonitrile with internal standard to incubation aliquots, followed by protein sedimentation by centrifuging at 5500 rpm for 5 minutes. Supernatants are analyzed using the HPLC-MS/MS system coupled with a tandem mass spectrometer. The elimination constant (kel), half-life (t1/2), and intrinsic clearance (Clint) are determined in a plot of ln(AUC) versus time, using linear regression analysis:
Comparison of Proprioceptive Nest with Standard Nesting to meet the proprioceptive needs of preterm neonates in NICU- A randomized control non-inferiority study v1
Authors: HEMANT NANDGAONKAR
Published: December 2024

Overview and Justification An essential component of neurodevelopment, proprioceptive stimulation begins in the foetal stage. Movements of the body can trigger sensorimotor experiences as early as the foetal stage. Proprioceptive feedback is created when the foetus moves within the uterus. The Proprioceptive Nest is a cutting-edge nesting tool made to give preterm infants comfort and age-appropriate proprioceptive stimulation. In preterm neonates under 36 weeks admitted to the NICU, the study aims to evaluate the effectiveness of the newly constructed device with standard nesting practices.
Rat organotypic cultures for AAV-mediated vital labeling of the extracellular matrix v2
Authors: N. Soria Federico , Fernandez-Ballester Mario
Published: December 2024

In this protocol we describe the preparation and maintenance of rat organotypic cultures (cortico-striatal) for AAV-infection and imaging of the extracellular matrix. The AAV, in this case, induces the expression of a exportable hyaluronan-binding protein (the HA-biding domain of Neurocan) fused with GFP, that allows vital labeling (and hence live imaging) of the extracellular matrix. The organotypic cultures can be maintained for several weeks, allowing for repeated imaging and longitudinal studies.
Directed cardiac lineage differentiation of human pluripotent stem cells v2
Authors: Li Fan
Published: December 2024

Robust cardiac lineage differentiation from human pluripotent stem cells (hPSCs) holds great potential for regenerative medicine, disease modeling, and drug discovery. Adapted from previous protocols (Proc Natl Acad Sci U S A. 2012 Jul 3;109(27), PMID: 22645348; Cell Stem Cells, 2013 Jan 3;12(1):127-37, PMID: 23168164; Nature biotechnology. 2007 Sep;25(9):1015-24. PMID: 17721512; Nature protocols. 2017 Jan;12(1):15-31. PMID: 27906170), we present a robust monolayer protocol for efficiently generating both cardiomyocytes and cardiac fibroblasts from multiple human embryonic stem cell (hESC) and human induced pluripotent stem cell (hiPSC) lines. This method involves the sequential modulation of key signaling pathways to mimic the stages of cardiac development. Prior to differentiation, hPSCs are maintained in a defined pluripotent state using a feeder-free culture system. Cardiac lineage differentiation is initiated by the temporal inhibition of GSK3, followed by treatment with Activin A and BMP4, directing these hPSCs toward a cardiac fate. These cardiomyocytes display spontaneous contractile activity and express markers for both pan-cardiomyocytes (e.g. TNNT2) and ventricular cardiomyocyte fates (e.g. IRX4 and MYH7).
THE POPULATION SIZE AND THE SHARE OF UKRAINIANS IN IT ON THE OCCUPATION-FREE AND THE RUSSIAN OCCUPIED TERRITORIES OF KHERSON AND ZAPORIZHZHIA OBLASTS
Authors:   Rylsky Institute of Art History, Folklore and Ethnology of the National Academy of Sciences of Ukraine, Ukraine, SKLIAR Volodymyr
Published: December 2024

The study of ethnic processes in Ukraine is an important task of Ukrainian studies. In the context of the russian-Ukrainian war, it is especially urgent to refute russian mythologemes about the ethnic structure of the Ukrainian population. The article determines the area of the occupation-free and occupied territories of Kherson and Zaporizhzhia regions, taking into account the administrative-territorial structure according to the 2020 reform. The dynamics of the population of these territories is established based on the statistical materials of the 2001 All-Ukrainian Population Census, statistical data at the time of the 2020 administrative-territorial reform, and information from the Kherson and Zaporizhzhia regional military administrations on the population in 2024. Despite the fact that most of the territories of these regions were occupied, the smaller areas that were free of russian aggression in 2024 and had regional centers were home to the majority of the population of these regions before the russian aggression. The author proves that Ukrainians constitute an absolute majority in the ethnic composition of the population of Kherson and Zaporizhzhia regions, among the urban and rural population, and among the population of all its districts. Therefore, the russian claims to the territory of Kherson and Zaporizhzhia regions are groundless and insignificant in terms of the arguments for the annexation of the territories inhabited by Ukrainians in these southern regions of Ukraine. The directions and consequences of the Russian imperial policy on the occupied Ukrainian lands require further special research, as well as the introduction of criminal responsibility for the war crimes of the Russian invaders and collaborators on the part of Ukrainian and international justice.
Teacher burnout: psychological risk factors
Authors: Miniurova S. A. , Vorobyeva I. V. , Kruzhkova О. V.
Published: December 2024

Introduction. The definition of psychological predictors of teacher emotional burnout can enhance scientific understanding of the specific factors contributing to its occurrence in contemporary pedagogical contexts. Additionally, it can identify the individual psychological characteristics of educators that are most susceptible to burnout, thereby facilitating targeted interventions that will aid in the development of effective preventive measures.

Aim. The present research aimed to identify and describe the psychological risk factors associated with the occurrence of emotional burnout among teachers.

Methodology and research methods. The study of emotional burnout and the psychological characteristics of teachers’ personalities was conducted remotely using the Yandex Forms tool in 2024. A total of 1,054 educators participated in the study. The diagnostic tools package included the following methods: the Emotional Burnout Diagnosis (V. V. Boyko), the Emotional Intelligence Inventory – EmIn (D. V. Lyusin), the Integrative Anxiety Test (A. P. Bizyuk, L. I. Wasserman, B. V. Iovlev), the Motivation of Professional Activity Diagnosis (K. Zamfir, modified by A. Rean), and a methodology for assessing overall job satisfaction. The mathematical and statistical analysis of the results was conducted using descriptive statistics and linear regression analysis, which involved both forced inclusion and sequential exclusion of independent variables.

Results. The research findings indicate that the issue of emotional burnout syndrome is indeed a pressing concern for the pedagogical community. Specifically, 16.3% of respondents exhibit a phase of tension, 40% demonstrate a pronounced phase of resistance, and 19.8% of teachers have been diagnosed with a developed phase of exhaustion. Based on regression analysis, a model of psychological risk factors contributing to teachers’ emotional burnout has been constructed.

Scientific novelty. The scientific novelty lies in the examination of emotional burnout not only in terms of its prevalence among educators in various educational institutions but also in identifying potential predictors that may serve as risk factors for the development of this professional deformation among teaching staff.

Practical significance arises from the potential to utilise the obtained results in developing targeted measures to prevent emotional burnout among teachers.
Escenarios y alternativas de restauración ecológica en paisajes andinos degradados: el caso de la cuenca alta del río Bogotá (Suesca, Cundinamarca)
Authors: Ruiz-Marín Esperanza , Peña Alejandro , Hernández-Abril Sabina Alejandra , Pachón-Blanco Lina María , Vargas-Ríos Orlando , Rodriguez-Eraso Nelly
Published: December 2024

La intensificación agrícola y la expansión urbana han provocado la pérdida de hábitat en ecosistemas tropicales, afectando negativamente la biodiversidad, los servicios ecosistémicos y las condiciones de vida de las personas. En la región andina de Colombia, la dinámica socioecológicay la fragmentación del paisaje hacen necesarios enfoques integrales de restauración ecológica queinvolucren a la comunidad para mejorar la resiliencia y sostenibilidad del territorio. Este estudiointegra una metodología participativa, cuyos resultados se derivaron de encuestas, entrevistas,talleres y recorridos de campo. Se determinaron y tipificaron unidades y estrategias de restauraciónfrente a dos escenarios participativos: la conectividad ecológica y la transición agroecológica, quebuscan recuperar la biodiversidad y los procesos ecológicos relacionados con el agua, el suelo,la agricultura y la ganadería (polinización, control natural de plagas, confort térmico) asociandoestrategias específicas de restauración y especies vegetales. Concluimos que los escenariosparticipativos fomentan la gestión comunitaria del territorio en pro de soluciones colaborativas entorno a la restauración ecológica. Consideramos que el estudio puede replicarse en otros paisajes andinos degradados, donde el conocimiento de la comunidad y el acompañamiento de la academia son esenciales para mejorar la efectividad de la restauración
Analyzing the Chinese public attention to the COVID-19 pandemic using Sina Weibo, a microblogging website
Authors: Teng Shuai , Xu Jinping , Sun Xiaodong , Yu Fangxin , Hao Yuting
Published: December 2024

The outbreak of COVID-19 in China captured the attention of the whole country. People used social media to search for information about the pandemic and to publish their own thoughts. To explore people’s knowledge and feelings about COVID-19, this study used Python to crawl popular microblogs with the keyword ‘new coronavirus pneumonia’ between 21 January 2020 and 18 March 2020 and analyzed the sentiment tendency using ROST CM6 on the popular microblogging platform, Sina Weibo. The study used Python to capture a total of 34,585 popular microblogs over 58 days, including usernames, number of comments, number of retweets, and contents. The results showed the following: 1) The public consistently paid close attention to the COVID-19 pandemic; 2) Throughout the pandemic, high-frequency words at different stages have partially changed; 3) People generally held a negative view of the pandemic, although performance tended toward a positive change; 4) The number of highly interactive microblogs released by the media is much higher than those of other bloggers, and the total number of reposts and comments on highly interactive microblogs released by the official media was much higher than that of ordinary media. The results of our study demonstrate that the media, especially the official media, played a vital role in broadcasting pandemic information, while the government microblog (which is also the official source of information) played a minimal role. Therefore, further research should focus on persuading government microblogs to communicate more effectively during a pandemic.
Domestic violence and reproductive health: problems of update of state legal policy
Authors: Blikhar M.M. , Andrusіak I.Р. , Khytra O.L. , Hula I.L. , Pasternak V.М.
Published: December 2024

Objective of the study: to study the problem of updating the legal policy of the state within the scope of domestic violence and women’s reproductive health.
Materials and methods. The study was carried out from 2021 to 2023 in 4 regional centers of Ukraine: Lviv, Uzhgorod, Chernihiv and Kyiv. A survey of 712 people was conducted using structured questions, which were aimed at studying various aspects of social perception of legal policy regarding domestic violence and its manifestations during a woman’s pregnancy. Two focus groups were subject to analysis: the first group included 580 pregnant and non-pregnant women of various ages, the second group included 132 students of senior years of medical universities.
Results. The results of the survey proved that pregnant women also become victims of domestic violence, especially from their intimate partner, but the level of physical and sexual violence is reduced during this period. The number of births significantly affects the frequency of domestic violence: respondents stated that the fourth or more births significantly increased the risk of becoming a victim of violence. The need to update the legal policy in the field of medical education is substantiated. However, the lack of education of health workers regarding violence emphasizes the need for updating approaches. Therefore, emphasis is placed on the core task of medical personnel - to cooperate with law enforcement agencies and other services including social to ensure the safety of victims and provide them with the necessary support.
Conclusions. Based on statistical indicators, scientific doctrine in the field of medicine and law (Australia, Great Britain, USA, Iran, Norway, Latin America), it can be summarized that violence during pregnancy has primary (somatic) and long-term (psychological) consequences. Although these problems largely depend on socio-mental factors and the legal policy of the state, they still remain a serious threat to women’s health and reproductive function.