bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of Mixing Hippophae rhamnoides and Pinus tabuliformis on Ecosystem Carbon, Nitrogen, and Phosphorus Sequestration and Storage Capacity in the Loess Hilly Region, China
Authors: Yu Wenwei , Jiang Rumeng , Wei Xiaolan , Luan Xinlong , Chen Yueyan , Ma Baohong , Wu Xu , Niu Yaobin , Chen Yunming
Published: August 2024

Mixed forests created by incorporating nitrogen-fixing tree species offer enhanced ecological advantages compared with forests consisting of only one type of tree species. These benefits include habitat rehabilitation and the promotion of biodiversity. Nevertheless, the impact of introducing nitrogen-fixing tree species on ecosystem carbon (C), nitrogen (N), and phosphorus (P) sequestration and storage capacity in the Loess Plateau of China remains inadequately explored. To examine changes in the sequestration and storage capacity of ecosystem C, N, and P, the mixed plantations of P. tabulaeformis and H. rhamnoides (HrPt) were selected as the research object, and the pure plantations of H. rhamnoides (Hr) and P. tabulaeformis (Pt) were selected as the control. The results indicated that in comparison to the pure forest, the ecosystem in HrPt significantly increased C and N stocks but decreased P stocks. In addition, C, N, and P stocks in the soil layer accounted for more than 60% of the C, N, and P stocks in the pure and mixed forest ecosystems compared with the vegetation layer. Moreover, HrPt significantly improved ecosystem C and N sequestration rates relative to the pure forest but decreased P sequestration rates. Furthermore, the soil physicochemical properties can be inferred from the redundancy analysis showing 66.79% and 0.06% in Hr, 44.84% and 0.06% in Pt, as well as 44.28% and 0.04% in HrPt, respectively. In conclusion, compared with the pure forest, the introduction of N-fixing tree species was more conducive to the accumulation of C and N. The results offer substantial significance for the scientific guidance of vegetation restoration in degraded landscapes and the stewardship of mixed forests in the Loess Hilly Region, providing essential data for nutrient storage in ecosystems.
Effective Ethyl Carbamate Prevention in Red Wines by Treatment with Immobilized Acid Urease
Authors: Tavilli Elisa , Esti Marco , Fidaleo Marcello
Published: August 2024

Climate change poses several challenges in the wine industry, including increasing risks related to chemical food contaminants such as biogenic amines and ethyl carbamate (EC). In this work, we focused on urea removal in red wines by immobilized acid urease aiming at limiting EC formation during wine storage. By considering separable kinetics of catalyst deactivation and urea hydrolysis, it was possible to model the time course of urea removal in repeated uses in stirred batch reactors. Treatments based on immobilized urease of red wine enriched with 30 mg/L of urea allowed the reduction in the contaminant concentration to <5 mg/L. After 28.5 h of treatment, the observed urea level was reduced to about 0.5 mg/L, corresponding to a decrease in the potential ethyl carbamate (PEC) from 1662 μg/L to 93 μg/L, below the level of the non-enriched wine (187 μg/L). As a comparison, when treating the same wine with the free enzyme at maximum doses allowed by the EU law, urea and PEC levels decreased to only 12 mg/L and 415 μg/L respectively, after 600 h of treatment. These results show that, for red wines, urease immobilization is an effective strategy for urea removal and, thus, effective reduction in ethyl carbamate as a process contaminant. This study provides the scientific background for the future scaling-up of the process at an industrial level.
In Vitro Antioxidant Activity of Liposomal Formulations of Sea Buckthorn and Grape Pomace
Authors: Popovici Violina , Boldianu Adrian-Bogdan , Pintea Adela , Caraus Vladimir , Ghendov-Mosanu Aliona , Subotin Iurie , Druta Raisa , Sturza Rodica
Published: August 2024

This study evaluated the impact of the encapsulation of sea buckthorn and grape pomace extracts in liposomal formulations on the retention and release of bioactive compounds and their antioxidant activity. The profile and composition of lipophilic extracts of sea buckthorn and hydrophilic extracts of grape pomace were analyzed. Encapsulation efficiency, retention rate, and the content of bioactive compounds encapsulated in liposomal formulations prepared in two media—water and ethanol—were evaluated. The encapsulation efficiency varied between 84 and 90%, indicating the superior encapsulation of the bioactive compounds. The retention rate varied between 79 and 86%, which indicated the stability of the liposome-encapsulated compounds over time. The antioxidant activity of the encapsulated samples was determined in vitro, under the conditions of gastric (pH 1.8) and intestinal (pH 8.2) digestion, in relation to the non-encapsulated extracts. The antioxidant activity of both liposomal formulations was higher than that of the nonencapsulated extracts during gastric digestion. Moreover, an increase over time in the antioxidant activity, expressed as % DPPH inhibition, was observed for all samples, with around 90% DPPH inhibition for non-encapsulated extracts and 92% for the encapsulated extracts, demonstrating the stability of bioactive compounds in acidic pH. Oppositely, when exposed to intestinal simulated digestion (alkaline pH), the antioxidant activity decreased over time to around 24% DPPH inhibition for both encapsulated and nonencapsulated extracts. These results provide a foundation for the further development and application of liposomal delivery systems in functional foods.
Global Mittag-Leffler Attractive Sets, Boundedness, and Finite-Time Stabilization in Novel Chaotic 4D Supply Chain Models with Fractional Order Form
Authors: Johansyah Muhamad Deni , Sambas Aceng , Farman Muhammad , Vaidyanathan Sundarapandian , Zheng Song , Foster Bob , Hidayanti Monika
Published: August 2024

This research explores the complex dynamics of a Novel Four-Dimensional Fractional Supply Chain System (NFDFSCS) that integrates a quadratic interaction term involving the actual demand of customers and the inventory level of distributors. The introduction of the quadratic term results in significantly larger maximal Lyapunov exponents (MLE) compared to the original model, indicating increased system complexity. The existence, uniqueness, and Ulam–Hyers stability of the proposed system are verified. Additionally, we establish the global Mittag-Leffler attractive set (MLAS) and Mittag-Leffler positive invariant set (MLPIS) for the system. Numerical simulations and MATLAB phase portraits demonstrate the chaotic nature of the proposed system. Furthermore, a dynamical analysis achieves verification via the Lyapunov exponents, a bifurcation diagram, a 0–1 test, and a complexity analysis. A new numerical approximation method is proposed to solve non-linear fractional differential equations, utilizing fractional differentiation with a non-singular and non-local kernel. These numerical simulations illustrate the primary findings, showing that both external and internal factors can accelerate the process. Furthermore, a robust control scheme is designed to stabilize the system in finite time, effectively suppressing chaotic behaviors. The theoretical findings are supported by the numerical results, highlighting the effectiveness of the control strategy and its potential application in real-world supply chain management (SCM).
WRKY10 Regulates Seed Size through the miR397a-LAC2 Module in Arabidopsis thaliana
Authors: Guo Wenbin , Yang Ke , Ye Hang , Yao Jialing , Li Jing
Published: August 2024

In angiosperms, seed size is a critical trait that is influenced by the complex interplay between the endosperm and seed coat. The HAIKU (IKU) pathway, involving the transcription factor WRKY10, plays a crucial role in regulating seed size in Arabidopsis thaliana. However, the downstream targets of WRKY10 and their roles in seed size determination remain largely unexplored. Here, we identified LACCASE2 (LAC2), a laccase gene involved in lignin biosynthesis, as a new downstream target of WRKY10. We observed that the expression of LAC2 was upregulated in the mini3 mutant, which is defective in WRKY10. We demonstrated that WRKY10 directly binds to the promoter of miR397a, activating its expression. miR397a, in turn, represses the expression of LAC2. Genetic analyses revealed that a mutation in LAC2 or overexpression of miR397a partially rescued the small seed phenotype of the MINISEED3 (MINI3) mutant mini3. Conversely, the overexpression of LAC2 in the wild type led to a decrease in seed size. These findings suggest that LAC2 functions as a negative regulator of seed size, and its expression is modulated by WRKY10 through miR397a. Our study uncovers a novel WRKY10-miR397a-LAC2 pathway that regulates seed size in Arabidopsis, providing new insights into the complex regulatory network governing seed development in plants.
Morphogenesis, Structure, and Tillering Dynamics of Tanzania Grass under Nitrogen Fertilization in the Amazon Region
Authors: Lage Filho Nauara Moura , Santos Airton da Conceição dos , Silva Suianne Lorena da Silva e , Oliveira João Victor Costa de , Macedo Vitor Hugo Maués , Cunha Antônio Marcos Quadros , do Rêgo Aníbal Coutinho , Cândido Ebson Pereira
Published: August 2024

Nitrogen fertilization is one of the main management strategies for continuous pasture management with high productivity. We examined the effects of nitrogen fertilization on the morphogenic, structural, and tillering dynamic characteristics of Tanzania grass in the Amazon region in the state of Pará, Brazil. The study was conducted using a randomized block design with six treatments (0, 100, 200, 300, 400, and 500 kg N ha−1 year−1) and five replicates. The treatments were performed during the rainy season in 2016 and 2017 using urea as the nitrogen source. The leaf elongation rate was increased by 68.5% compared with that of the control treatment (p < 0.01). The leaf appearance rate and number of alive leaves increased with higher doses of nitrogen (p < 0.01, each). The regrowth period was reduced by approximately 13 days under 500 kg N ha−1 (p < 0.01), thus providing more production cycles. Nitrogen fertilization was also associated with a higher tillering rate (p < 0.01); however, the rate of this increase decreased with increasing nitrogen dose. Higher nitrogen doses thus improved the development of Tanzania grass; however, this became less pronounced at doses < 300 kg N ha−1.
Perceived Relational Empathy and Resilience in People with Spinal Cord Injury at the End of Acute Care: A Cross-Sectional Study
Authors: Zackova Monika , Rucci Paola , Di Staso Rossana , Ceretti Silvia , Bonavina Giuseppe , Delmestro Eric
Published: August 2024

In patients with spinal cord injury (SCI), patient-reported outcomes (PROMs) and experience of care measures (PREMs) are extremely relevant for the prognosis. However, there is a paucity of research on these topics. We conducted a cross-sectional study to investigate the relationships between these patient outcomes and other demographic and clinical variables in adult SCI patients discharged from the intensive care unit of an Italian tertiary rehabilitation hospital. We administered the Consultation and Relational Empathy (CARE) for perceived relational empathy, the Spinal Cord Independence Measure III self-report (SCIM-SR) for functional autonomy, the Numeric Rating Scale (NRS) for pain, and the Connor–Davidson Resilience Scale (CD-RISC-10) for resilience. Study participants consisted of 148 adults with SCI; 82.4% were male, with a mean age of 49.9 years (SD = 16.6). The lesion was traumatic in 82.4% and complete in 74.3% of cases. The median length of hospital stays was 35 days (interquartile range—IQR = 23–60). Perceived relational empathy was positively associated with resilience (r = 0.229, p = 0.005) and negatively associated with the length of the stay and lesion completeness. Resilience had a weak negative association with pain (r = −0.173, p = 0.035) and was unrelated to other variables. Clinicians should consider the routine assessment of PREMs and PROMs in order to personalize post-discharge therapeutic plans and identify appropriate measures to ensure continuity of care.
Correction: Zamfir et al. Trends in Coronary Artery Anomalies Detection by Coronary Computed Tomography Angiography (CCTA): A Real-Life Comparative Study before and during the COVID-19 Pandemic. Healthcare 2024, 12, 1091
Authors: Zamfir Alexandra-Simona , Cernomaz Tudor-Andrei , Ciuntu Bogdan Mihnea , Azoicăi Doina , Zamfir Carmen Lăcrămioara , Chistol Raluca Ozana , Sava Anca
Published: August 2024

In the original publication [...]
Comparative Evaluation of Temporomandibular Joint Parameters in Unilateral and Bilateral Cleft Lip and Palate Patients Using Cone-Beam CT: Focus on Growing vs. Non-Growing Subjects
Authors: Abdelkarim Ahmed Z. , Almeshari Ahmed A. , Ozen Duygu Celik , Khalifa Ayman R. , Rezallah Nader N. , Duman Suayip Burak , Khurana Sonam
Published: August 2024

Background: Morphological differences in the temporomandibular joint (TMJ) are crucial for the treatment of patients with cleft lip and palate (CLP). This study aims to evaluate and compare the TMJ parameters in patients with unilateral and bilateral CLP across growing and non-growing age groups using cone-beam computed tomography (CBCT). Methods: CBCT records from 57 patients (23 males and 34 females) aged 6–50 years with a diagnosed unilateral or bilateral CLP were analyzed. Patients were categorized into four groups: growing unilateral (UGCLP), growing bilateral (BGCLP), non-growing unilateral (UNGCLP), and non-growing bilateral (BNGCLP). Measurements of TMJ parameters, including the mandibular fossa, articular eminence inclination, joint spaces, and roof thickness of the glenoid fossa, were conducted using CBCT images. Results: Significant differences were observed in the anterior joint space (AJS) and the roof of the glenoid fossa (RGF) between growing and non-growing unilateral cleft patients. Additionally, significant discrepancies were found in the articular eminence angle when comparing the cleft and non-cleft sides within the unilateral growing group. No significant differences were observed in TMJ parameters between the right and left sides among bilateral cleft patients. Conclusions: The study highlights distinct TMJ morphological differences between growing and non-growing patients with CLP, emphasizing the importance of age-specific considerations in the treatment planning and growth monitoring of these patients.
EWS-FLI1 and Activator Protein-1 (AP-1) Reciprocally Regulate Extracellular-Matrix Proteins in Ewing sarcoma Cells
Authors: Croushore Emma E. , Stipp Christopher S. , Gordon David J.
Published: August 2024

Ribonucleotide reductase (RNR) is the rate-limiting enzyme in the synthesis of deoxyribonucleotides and the target of multiple chemotherapy drugs, including gemcitabine. We previously identified that inhibition of RNR in Ewing sarcoma tumors upregulates the expression levels of multiple members of the activator protein-1 (AP-1) transcription factor family, including c-Jun and c-Fos, and downregulates the expression of c-Myc. However, the broader functions and downstream targets of AP-1, which are highly context- and cell-dependent, are unknown in Ewing sarcoma tumors. Consequently, in this work, we used genetically defined models, transcriptome profiling, and gene-set -enrichment analysis to identify that AP-1 and EWS-FLI1, the driver oncogene in most Ewing sarcoma tumors, reciprocally regulate the expression of multiple extracellular-matrix proteins, including fibronectins, integrins, and collagens. AP-1 expression in Ewing sarcoma cells also drives, concurrent with these perturbations in gene and protein expression, changes in cell morphology and phenotype. We also identified that EWS-FLI1 dysregulates the expression of multiple AP-1 proteins, aligning with previous reports demonstrating genetic and physical interactions between EWS-FLI1 and AP-1. Overall, these results provide novel insights into the distinct, EWS-FLI1-dependent features of Ewing sarcoma tumors and identify a novel, reciprocal regulation of extracellular-matrix components by EWS-FLI1 and AP-1.
Zebrafish Models in Toxicology and Disease Studies
Authors: Ferrandino Ida
Published: August 2024

Danio rerio is a small tropical freshwater fish, also known as Brachydanio rerio and commonly referred to as zebrafish, described for the first time in 1822 by Francis Hamilton in the Ganges River but widespread throughout the entire Great Himalayan region of Southeast Asia [...]
VoiceBack: Design of Artificial Intelligence-Driven Voice-Based Feedback System for Customer-Agency Communication in Online Travel Services
Authors: Edén Anniki Skeidsvoll , Sandlund Pernilla , Faraon Montathar , Rönkkö Kari
Published: August 2024

Online travel booking has become increasingly popular; however, most travel websites do not yet offer voice interaction. This study introduces VoiceBack, an artificial intelligence (AI)-driven voice-based feedback system conceptualized to support both customers and online travel agencies during the booking process. It proposes a theoretically and empirically underpinned design concept that involves a voice user interface (VUI) for customer feedback. This feedback, collected by an AI agent, is analyzed and converted into actionable statistics, which are then presented to online travel agencies through a visual interface. The interface is designed to highlight problem areas and usability issues during the booking process. This study contributes to the field of human-centered AI, by offering insight into the complex process of designing and integrating voice, emotion, and feedback within user interfaces. This integrated approach can enrich the user experience of customers when booking travel online, and pave the way for more intuitive and responsive interaction designs in the future.
Elevating Security in Migration: An Enhanced Trusted Execution Environment-Based Generic Virtual Remote Attestation Scheme
Authors: Yuan Jie , Shen Yinghua , Xu Rui , Wei Xinghai , Liu Dongxiao
Published: August 2024

Cloud computing, as the most widely applied and prominent domain of distributed systems, has brought numerous advantages to users, including high resource sharing efficiency, strong availability, and excellent scalability. However, the complexity of cloud computing environments also introduces various risks and challenges. In the current landscape with numerous cloud service providers and diverse hardware configurations in cloud environments, addressing challenges such as establishing trust chains, achieving general-purpose virtual remote attestation, and ensuring secure virtual machine migration becomes a crucial issue that traditional remote attestation architectures cannot adequately handle. Confronted with these issues in a heterogeneous multi-cloud environment, we present a targeted solution—a secure migration-enabled generic virtual remote attestation architecture based on improved TEE. We introduce a hardware trusted module to establish and bind with a Virtual Root of Trust (VRoT), addressing the challenge of trust chain establishment. Simultaneously, our architecture utilizes the VRoT within TEE to realize a general-purpose virtual remote attestation solution across heterogeneous hardware configurations. Furthermore, we design a controller deployed in the trusted domain to verify migration conditions, facilitate key exchange, and manage the migration process, ensuring the security and integrity of virtual machine migration. Lastly, we conduct rigorous experiments to measure the overhead and performance of our proposed remote attestation scheme and virtual machine secure migration process. The results unequivocally demonstrate that our architecture provides better generality and migration security with only marginal overhead compared to other traditional remote attestation solutions.
Could Vaccination against COVID-19 Trigger Immune-Mediated Inflammatory Diseases?
Authors: Liakou Aikaterini I. , Tsantes Andreas G. , Routsi Eleni , Agiasofitou Efthymia , Kalamata Magdalini , Bompou Evangelia-Konstantina , Tsante Konstantina A. , Vladeni Soultana , Chatzidimitriou Eleni , Kotsafti Ourania , Samonis George , Bonovas Stefanos , Stratigos Alexander I.
Published: August 2024

Exacerbations and new onset of immune-mediated inflammatory diseases, such as psoriasis and hidradenitis suppurativa, have been reported following COVID-19 vaccination. In patients with hidradenitis suppurativa, recent studies have shown that those who received mRNA vaccines were 3.5 times as likely to develop flares following vaccination compared to patients who received non-mRNA vaccines, indicating that mRNA COVID-19 vaccines are associated with hidradenitis suppurativa flares. Similar findings have been found in other studies evaluating the association between COVID-19 vaccines and other immune-mediated inflammatory diseases such as psoriasis, atopic dermatitis, lichen planus, and alopecia areata. However, further research is warranted in larger populations to validate these findings.
Coupling Coordination Relationship between Cultural Landscape Conservation and Socio-Economic System in Ethnic Villages of Southeast Guizhou
Authors: Yang Mengling , Wu Chong , Gong Lei , Tan Guowei
Published: August 2024

Amidst the transition from agriculture to tourism, ethnic villages are facing issues of “misalignment” and “disequilibrium” between economic growth and cultural heritage conservation. Previous research has often isolated the effects of cultural heritage conservation or socio-economic development, neglecting their reciprocal influences within the coupling coordination. This study addresses this gap by assessing 43 villages in Leishan County, quantifying the preservation status of the cultural landscape (PSCL) and socio-economic level (SEL) using a comprehensive evaluation model and revealing the coupling coordination relationship between PSCL and SEL in each village with the coupling coordination degree (CCD) model. Spatial autocorrelation and the geographical detector model reveal the spatial agglomeration characteristics and influential factors of the CCD. The results highlight three findings. (1) The majority of villages (93%) are in a moderate imbalance stage or a serious imbalance stage, underscoring an urgent need for targeted policies. (2) Spatial autocorrelation analysis exposes significant clustering, with the northwest corner exhibiting the most pronounced high-value aggregation, which contrasts with the southern region’s low-value clustering. A ‘low–high’ clustering pattern is observed in the northern region, while the southwestern corner presents a ‘high–low’ distribution. (3) Key determinants affecting the CCD include the tourism revenue, tourist volume, resident participation in tourism, village hollowing level, and number of historical buildings. The interactions between driving factors show non-linear and two-factor enhancement effects. This study concludes with policy recommendations advocating for context-specific strategies to enhance the CCD, emphasizing the importance of aligning cultural preservation with economic progress in ethnic villages.
Breaking the Boundary between Permanent Capital Farmland and Arable Land in China: Understanding State and Drivers of Permanent Capital Farmland Non-Grain Production in a Rapid Urbanizing County
Authors: Shi Yunjie , Li Hengpeng , Geng Jianwei , Askar Akida , Zhao Zhongjing , Pang Jiaping , Zhang Wangshou , Shao Yuyang
Published: August 2024

Amid unprecedented challenges to protect arable land, China’s permanent capital farmland (PCF) has played a crucial role in grain production. However, a clear legal and physical boundary between PCF and arable land seems to be unable to stop the spread of non-grain production. To address it, an analysis framework for PCF non-grain production was developed to examine the state and drivers of village-scale PCF non-grain production based on the logical relationship between PCF and arable land in the rapid urbanization of Liyang. The results suggested that PCF comprised approximately 70% arable land and 30% adjustable land. Meanwhile, forest land and aquaculture ponds occupied over 25% of PCF, while nearly 20% of PCF is unsuitable for the resumption of crop cultivation. The transition state (scenario SR) offered a realistic representation of PCF non-grain production, with an average non-grain production of 48.88%. This is 14.00% lower than the current state (scenario SD) and 9.65% higher than the future state (scenario ST). Furthermore, PCF area and agricultural income per capita significantly encouraged PCF non-grain production, with explanatory powers of 51.60% and 42.40%, respectively. In contrast, urbanization rate (with an explanatory power of 35.30%) significantly discouraged it. Therefore, this paper proposed PCF redefinition, flexible PCF, and diversified economic incentives to mitigate PCF non-grain production at the village scale.
Correlation between Dental Composite Filler Percentage and Strength, Modulus, Shrinkage Stress, Translucency, Depth of Cure and Radiopacity
Authors: Lopez Carolina , Nizami Bushra , Robles Augusto , Gummadi Snigdha , Lawson Nathaniel C.
Published: August 2024

Filler content in dental composites is credited for affecting its physical and mechanical properties. This study evaluated the correlation between the filler percentage and strength, modulus, shrinkage stress, depth of cure, translucency and radiopacity of commercially available high- and low-viscosity dental composites. Filler weight percentage (wt%) was determined through the burned ash technique (800 °C for 15 min). Three-point bend flexural strength and modulus were measured according to ISO 4049 with 2 mm × 2 mm × 25 mm bars. Shrinkage stress was evaluated using a universal testing machine in which composite was polymerized through two transparent acrylic rods 2 mm apart. Shrinkage was measured from the maximum force following 500 s. The translucency parameter (TP) was measured as the difference in color (ΔE00) of 1 mm thick specimens against white and black tiles. The depth of cure was measured according to ISO 4049 in a cylindrical metal mold (4 mm diameter) with a 10 s cure. Radiopacity was measured by taking a digital X-ray (70 kVp for 0.32 s at 400 mm distance) of 1 mm thick specimens and comparing the radiopacity to an aluminum step wedge using image analysis software. The correlation between the filler wt% and properties was measured by Pearson’s correlation coefficient using SPSS. There was a positive linear correlation between the filler wt% and modulus (r = 0.78, p < 0.01), flexural strength (r = 0.46, p < 0.01) and radiopacity (r = 0.36, p < 0.01) and negative correlation with translucency (r = −0.29, p < 0.01). Filler wt% best predicts the modulus and strength and, to a lesser extent, the radiopacity and translucency. All but two of the high- and low-viscosity composites from the same manufacturer had statistically equivalent strengths as each other; however, the high-viscosity materials almost always had a statistically higher modulus. For two of the flowable composites measured from the same manufacturer (3M and Dentsply), there was a lower shrinkage stress in the bulk-fill version of the material but not for the other two manufacturers (Ivoclar and Tokuyama). All flowable bulk-fill composites achieved a deeper depth of cure than the flowable composite from the same manufacturer other than Omnichroma Flow Bulk.
Cryogenic Investigations into the Effect of Impact-Oscillatory Loading on Changes in the Mechanical Properties and Structural Condition of VT23M Two-Phase Titanium Alloy
Authors: Chausov Mykola , Hutsaylyuk Volodymyr , Maruschak Pavlo , Pylypenko Andrii , Karpets Myroslav , Shmanenko Vladyslav
Published: August 2024

Impact-oscillatory loading of variable intensity was applied to the VT23M high-strength sheet two-phase titanium alloy in liquid nitrogen. This was carried out to investigate the effect of this loading type on changes in the mechanical properties and structural condition of the alloy upon subsequent static tensioning at normal temperature. Dynamic non-equilibrium process (DNP) realized at a temperature of liquid nitrogen proved to be unable to impair the strength properties of the VT23M titanium alloy compared to room temperature; however, they caused a significant decrease in ductility (down to 16%). The impaired plastic properties of the alloy were shown to entail additional defects in the structural components of the alloy. The authors have found patterns of damage accumulation in the structural components of the alloy depending on the DNP parameters. They are in good agreement with the findings of the fractographic research.
An Expanded Spatial Durbin Model with Ordinary Kriging of Unobserved Big Climate Data
Authors: Falah Annisa Nur , Andriyana Yudhie , Ruchjana Budi Nurani , Hermawan Eddy , Harjana Teguh , Maryadi Edy , Risyanto  , Satyawardhana Haries , Sipayung Sinta Berliana
Published: August 2024

Spatial models are essential in the prediction of climate phenomena because they can model the complex relationships between different locations. In this study, we discuss an expanded spatial Durbin model with ordinary kriging on unobserved locations (ESDMOK) to predict rainfall patterns in Java Island. The classical spatial Durbin model needed to be expanded to obtain a parameter estimation for each location. We combined this with ordinary kriging because the data were not available in some locations. The data were taken from the National Aeronautics and Space Administration Prediction of Worldwide Energy Resources (NASA POWER) website. Since climate data are big data, we implement a big data analytics approach, namely the data analytics life cycle method. As the exogenous variables, we used air temperature, humidity, solar irradiation, wind speed, and surface pressure. The authors developed an R-Shiny web applications to implement our proposed technique. Using our proposed technique, we obtained more accurate and reliable climate data prediction, indicated by the mean absolute percentage error (MAPE), which was equal to 1.956%. The greatest effect on rainfall was given by the surface pressure variable, and the smallest was wind speed.
Graphene Oxide Surface Modification of Reverse Osmosis (RO) Membrane via Langmuir–Blodgett Technique: Balancing Performance and Antifouling Properties
Authors: Petukhov Dmitrii I. , Weston James , Valeev Rishat G. , Johnson Daniel J.
Published: August 2024

The reverse osmosis water treatment process is prone to fouling issues, prompting the exploration of various membrane modification techniques to address this challenge. The primary objective of this study was to develop a precise method for modifying the surface of reverse osmosis membranes to enhance their antifouling properties. The Langmuir–Blodgett technique was employed to transfer aminated graphene oxide films assembled at the air–liquid interface, under specific surface pressure conditions, to the polyamide surface with pre-activated carboxylic groups. The microstructure and distribution of graphene oxide along the modified membrane were characterized using SEM, AFM, and Raman mapping techniques. Modification carried out at the optimal surface pressure value improved the membrane hydrophilicity and reduced the surface roughness, thereby enhancing the antifouling properties against colloidal fouling. The flux recovery ratio after modification increased from 65% to 87%, maintaining high permeability. The modified membranes exhibited superior performance compared to the unmodified membranes during long-term fouling tests. This membrane modification technique can be easily scaled using the roll-to-roll approach and requires minimal consumption of the modifier used.
A Comprehensive Review of Laser Powder Bed Fusion in Jewelry: Technologies, Materials, and Post-Processing with Future Perspective
Authors: Thangamani Geethapriyan , Felicioni Stefano , Padovano Elisa , Biamino Sara , Lombardi Mariangela , Ugues Daniele , Fino Paolo , Bondioli Federica
Published: August 2024

In recent years, additive manufacturing (AM) has played a significant role in various fashion industries, especially the textile and jewelry manufacturing sectors. This review article delves deeply into the wide range of methods and materials used to make intricately designed jewelry fabrication using the additive manufacturing (AM) process. The Laser Powder Bed Fusion (L-PBF) process is examined for its suitability in achieving complex design and structural integrity in jewelry fabrication even with respect to powder metallurgy methods. Moreover, the review explores the use of precious materials, such as gold, silver, copper, platinum, and their alloys in additive manufacturing. Processing precious materials is challenging due to their high reflectivity and thermal conductivity, which results in poor densification and mechanical properties. To address this issue, the review article proposes three different strategies: (i) adding alloying elements, (ii) coating powder particles, and (iii) using low-wavelength lasers (green or blue). Finally, this review examines crucial post-processing techniques to improve surface quality, robustness, and attractiveness. To conclude, this review emphasizes the potential of combining additive manufacturing (AM) with traditional craftsmanship for creating jewelry, exploring the potential future directions and developments in the field of additive manufacturing (AM) for jewelry fabrication.
Series/Parallel Switching for Increasing Power Extraction from Thermoelectric Power Generators
Authors: Terashima Shingo , Sorimachi Ryuji , Iwase Eiji
Published: August 2024

We propose a method for increasing power extraction from a thermoelectric generator (TEG) by switching between series/parallel circuit configurations of thermoelectric elements, which can adjust the internal impedance of the TEG. The power characteristics of the TEG can be adjusted to the load characteristics of the connected device and the relevant ambient temperature. In this paper, we analyzed the change in the TEG characteristics with the series/parallel switching function. We evaluated the power supply to the connected devices at different ambient temperatures and different series/parallel configurations and confirmed that the extracted power could be increased. By theoretically analyzing the circuit configuration of the thermoelectric devices, the switching required to improve the power extraction, and the temperature difference at which switching occurred, we devised a design method for a TEG with circuit switching in order to increase power extraction with any device. We demonstrated the configuration of switching by using a system in which a TEG supplied power to an external wireless transmitter circuit. In this system, the optimal configuration differed at temperature differences of 3.0 K and 4.0 K. At a temperature difference of 3.0 K, the 2-series/1-parallel configuration provided 10% more power to the external circuit than the 1-series/2-parallel configuration. On the other hand, at the temperature difference of 4.0 K, the 1-series/2-parallel configuration provided 23% more power than the 2-series/1-parallel configuration.
Temporal–Spatial Fluctuations of a Phytoplankton Community and Their Association with Environmental Variables Based on Classification and Regression Tree in a Shallow Temperate Mountain River
Authors: Tian Wang , Wang Zhongyu , Kong Haifei , Tian Yonglan , Huang Tousheng
Published: August 2024

The effects of environmental factors on phytoplankton are not simply positive or negative but complex and dependent on the combination of their concentrations in a fluctuating environment. Traditional statistical methods may miss some of the complex interactions between the environment and phytoplankton. In this study, the temporal–spatial fluctuations of phytoplankton diversity and abundance were investigated in a shallow temperate mountain river. The machine learning method classification and regression tree (CART) was used to explore the effects of environmental variables on the phytoplankton community. The results showed that both phytoplankton species diversity and abundance varied fiercely due to environmental fluctuation. Microcystis aeruginosa, Amphiprora sp., Anabaena oscillarioides, and Gymnodinium sp. were the dominant species. The CART analysis indicated that dissolved oxygen, oxidation-reduction potential, total nitrogen (TN), total phosphorus (TP), and water temperature (WT) explained 36.00%, 13.81%, 11.35%, 9.96%, and 8.80%, respectively, of phytoplankton diversity variance. Phytoplankton abundance was mainly affected by TN, WT, and TP, with variance explanations of 39.40%, 15.70%, and 14.09%, respectively. Most environmental factors had a complex influence on phytoplankton diversity and abundance: their effects were positive under some conditions but negative under other combinations. The results and methodology in this study are important in quantitatively understanding and exploring aquatic ecosystems.
Antibacterial and Antibiofilm Effects of L-Carnitine-Fumarate on Oral Streptococcal Strains Streptococcus mutans and Streptococcus sobrinus
Authors: Goc Anna , Sumera Waldemar , Rath Matthias , Niedzwiecki Aleksandra
Published: August 2024

Streptococcus mutans is a major pathogenic habitant of oral caries. Owing to its physiological and biochemical features, it prevails in the form of plaque biofilm together with another important mutans streptococci species, Streptococcus sobrinus. Both species are considered as initiators of cavity lesions, and biofilm is essential to the dental caries process. Compared with the planktonic populations, the biofilm form has higher resistance to environmental conditions and antibiotics. Dental plaques also secure the long-term survival of microorganisms and protection from any stress conditions. To address the need for new antibiofilm agents, we have focused on L-carnitine-fumarate, a fumarate-conjugated quaternary ammonium compound. Using the macro-broth susceptibility testing method, we established its MIC value as 6.0 mg/mL. The MBC value, determined from the broth dilution minimum inhibitory concentration test by sub-culturing it to BHI agar plates, was established as 7.0 mg/mL. Antibiofilm efficacy was tested in 96-well plates coated with saliva using BHI broth supplemented with 1% sucrose as a standard approach. The obtained results allowed us to assess the MIBC as 7.5 mg/mL and the MBBC value as 10.0 mg/mL. The latter concentration also caused approximately 20% eradication of pre-existing biofilm. EPS-rich matrix, forming the core of the biofilm and enabling a confined acidic microenvironment, was also examined and confirmed the effectiveness of 10.0 mg/mL L-carnitine-fumarate concentration in inhibiting EPS formation. Furthermore, the anti-adherent and anti-aciduric impacts of L-carnitine-fumarate were investigated and revealed significant inhibitory effects at sub-MIC concentrations. The influence of L-carnitine-fumarate on the phosphotransferase system was investigated as well. Our results provide a new insight into the antibacterial potential of L-carnitine-fumarate as a valuable compound to be considered for alternative or adjunct anti-caries and antibiofilm preventive approaches.
Zinc and Lead Leaching from Sphalerite–Galena Concentrate Using Deep Eutectic Solvents Based on Choline Chloride: Effect of Roasting and Iodine as Oxidizing Agent
Authors: Moreno Katherine , Díaz Ximena , Endara Diana , Sánchez Fernando , Aragón-Tobar Carlos F.
Published: August 2024

The traditional metallurgical routes for producing lead and zinc from primary sources have a significant environmental footprint. Thus, using less pollutant solvents, such as deep eutectic solvents (DESs), would offer a greener solution in metal extraction. This study explores the use of three DESs based on choline chloride (ChCl) (1:2 ChCl–urea, 1:2 ChCl–ethylene glycol, and 1:2 ChCl–glycerol) for recovering Zn and Pb from a sphalerite–galena concentrate of the mining region in Ecuador. Leaching tests of the concentrate (untreated and roasted at 600 °C) in each DES were conducted (30 °C—24 h). The effect of adding iodine as an oxidizing agent was also evaluated. Recoveries of 2% (Zn) and 14% (Pb) were reported when leaching the untreated concentrate with DES. These recovery values increased to 11% (Zn) and 99% (Pb) after adding iodine during the leaching of the untreated concentrate. Roasting had a similar effect on leaching, increasing the recovery values of Zn (75%) and Pb (90%). Combining roasting as a pretreatment and iodine as an oxidizing agent produced higher Zn recoveries (99%) and Pb (99%). These results were compared to recoveries in acid leaching (H2SO4 and HNO3), revealing the potential of DESs as an alternative for metal recovery from primary sources.
Reduction in Serum Carotenoid Levels Following One Anastomosis Gastric Bypass
Authors: Harari Ayelet , Kaniel Osnat , Keshet Rom , Shaish Aviv , Kessler Yafit , Szold Amir , Langer Peter , Raziel Asnat , Sakran Nasser , Goitein David , Moran-Gilad Jacob , Sherf-Dagan Shiri
Published: August 2024

Given the health benefits of carotenoids, it is crucial to evaluate their levels in patients undergoing malabsorptive procedures like one anastomosis gastric bypass (OAGB). This study aimed to assess serum carotenoid levels before and 6 months following OAGB. Prospectively collected data from patients who underwent primary OAGB were analyzed. Data included anthropometrics, dietary intake assessments, and biochemical tests. Serum samples were analyzed for lipid profile and serum carotenoids, including lutein, zeaxanthin, α-carotene, β-carotene, phytofluene, ζ-carotene, and lycopene. Data from 27 patients (median age 47.0 years and 55.6% female) were available before and 6 months post-OAGB. The median pre-surgical BMI was 39.5 kg/m2, and the median excess weight loss at 6 months post-surgery was 63.9%. Significant decreases in all carotenoid levels were observed over time (p < 0.001 for all). A median relative decline of 65.1% in absolute total carotenoid levels and 12.7% in total cholesterol levels were found. No associations were observed between changes in clinical outcomes and carotenoid levels during the study period. This study reveals significant decreases in carotenoid levels within the first 6 months following OAGB. Nutritional intervention studies are needed to explore how incorporating carotenoid-rich foods affects post-surgery carotenoid levels and clinical outcomes.
Chrono-Nutrition, Chrono-Type, and the Prevalence of Type 2 Diabetes Mellitus in a Cross-Sectional Study from the EuroPean Prospective Investigation into Cancer and Nutrition (EPIC) Study
Authors: Luján-Barroso Leila , Margara-Escudero Hernando J. , Crous-Bou Marta , Huerta José María , Chirlaque María-Dolores , Molina-Montes Esther , Sánchez María José , Guevara Marcela , Moreno-Iribas Conchi , Amiano Pilar , Mokoroa Olatz , González Sonia , Agudo Antonio , Quirós José Ramón , Jakszyn Paula
Published: August 2024

Background: Previous studies have shown that meal timing, poor sleep quality, and chronotype may play a relevant role in the development of type 2 diabetes mellitus (T2DM). However, its relationship with macronutrients by eating occasions has not been explored deeply. Objective: Our aim was to estimate the association between chrono-nutrition, sleep quality, chronotype, and the prevalence of T2DM. Methods: This cross-sectional study included a subset of 3465 middle-aged Caucasian adults (2068 women) from the European Prospective Investigation into Cancer and Nutrition (EPIC) Spain cohort study. In the 2017–18 follow-up, we assessed chronotype, sleep quality, diet, and sociodemographic data using validated questionnaires. Further, we used blood samples to determine serum levels of glucose. We defined a case of T2DM when serum glucose concentration was ≥126 mg/dL or when participants self-reported diabetes. Results: A higher prevalence of T2DM was associated with poor sleep quality (ORpoorvsgood = 2.90, 95% CI = 1.30, 6.28). Carbohydrate intake at breakfast was inversely associated with the prevalence of T2DM (OR = 0.75, 95% CI = 0.66, 0.85). Finally, lipid intake at breakfast was associated with a 13% higher prevalence of T2DM (OR = 1.13, 95% CI = 1.01, 1.26) for each 1 standard deviation (1-SD) increase. Conclusions: This study concludes that a higher content of carbohydrates at breakfast is correlated with a reduced prevalence of T2DM, while higher lipids intake at breakfast is associated with a higher prevalence of T2DM. Furthermore, poor sleep quality is a potential factor associated with an elevated prevalence of T2DM. Our results emphasize the need for prospective studies to validate and strengthen these observed associations.
Anthocyanin-Rich Fraction of Black Rice Bran Extract Protects against Amyloid β-Induced Oxidative Stress, Endoplasmic Reticulum Stress, and Neuronal Apoptosis in SK-N-SH Cells
Authors: Sivasinprasasn Sivanan , Tocharus Jiraporn , Mahatheeranont Sugunya , Nakrat Sarun , Tocharus Chainarong
Published: August 2024

Alzheimer’s disease (AD) is the most common neurodegenerative disorder in the aging population. An accumulation of amyloid plaques and neurofibrillary tangles causes degeneration of neurons, leading to neuronal cell death. The anthocyanin-rich fraction of black rice (Oryza sativa L. variety “Luem Pua”) bran (AFBRB), extracted using a solution of ethanol and water and fractionated using Amberlite XAD7HP column chromatography, contains a high anthocyanin content (585 mg of cyanidin-3-O-glucoside and 24 mg of peonidin-3-O-glucoside per gram of the rich extract), which has been found to reduce neurodegeneration. This study focused on the neuroprotective effects of AFBRB in Aβ25–35-induced toxicity in the human neuroblastoma cell line (SK-N-SH). SK-N-SH was exposed to Aβ25–35 (10 µM) to induce an AD cell model in vitro. Pretreatment with AFBRB (0.1, 1, or 10 µg/mL) or C3G (20 µM) was conducted for 2 h prior to the treatment with Aβ25–35 (10 µM) for an additional 24 h. The results indicate that AFBRB can protect against the cytotoxic effect of Aβ25–35 through attenuation of intracellular ROS production, downregulation of the expression of the proteins Bax, cytochrome c, cleaved caspase-9, and cleaved caspase-3, upregulation of the expression of Bcl-2 in the mitochondrial death pathway, and reduction in the expression of the three major markers of ER stress pathways in similar ways. Interestingly, we found that pretreatment with AFBRB significantly alleviated Aβ-induced oxidative stress, ER stress, and apoptosis in SK-N-SH cells. This suggests that AFBRB might be a potential therapeutic agent in preventing neurodegenerative diseases.