bluuebunny/crossref_metadata_2025 huggingface dataset:
Establishment of a stable transfection and gene targeting system in Babesia divergens
Authors: Cubillos Eliana F. G. , Snebergerova Pavla , Borsodi Sarka , Reichensdorferova Dominika , Levytska Viktoriya , Asada Masahito , Sojka Daniel , Jalovecka Marie
Published: December 2023

Babesia divergens is an emerging tick-borne pathogen considered as the principal causative agent of bovine babesiosis in Europe with a notable zoonotic risk to human health. Despite its increasing impact, considerable gaps persist in our understanding of the molecular interactions between this parasite and its hosts. In this study, we address the current limitation of functional genomic tools in B. divergens and introduce a stable transfection system specific to this parasite. We define the parameters for a drug selection system hdhfr-WR99210 and evaluate different transfection protocols for highly efficient generation of transgenic parasites expressing GFP. We proved that plasmid delivery into bovine erythrocytes prior to their infection is the most optimal transfection approach for B. divergens, providing novel evidence of Babesia parasites’ ability to spontaneously uptake external DNA from erythrocytes cytoplasm. Furthermore, we validated the bidirectional and symmetrical activity of ef-tgtp promoter, enabling simultaneous expression of external genes. Lastly, we generated a B. divergens knockout line by targeting a 6-cys-e gene locus. The observed dispensability of this gene in intraerythrocytic parasite development makes it a suitable recipient locus for further transgenic application. The platform for genetic manipulations presented herein serves as the initial step towards developing advanced functional genomic tools enabling the discovery of B. divergens molecules involved in host-vector-pathogen interactions.
Characterization of retinal microvasculature and structure in atrial fibrillation
Authors: Liu Junfeng , Tao Wendan , Li Dayan , Kwapong William Robert , Cao Le , Zhang Xiaoling , Ye Chen , Chen Shi , Liu Ming
Published: December 2023

Background and objective

Quantitative changes in retinal microvasculature are associated with subclinical cardiac alterations and clinical cardiovascular diseases (i.e., heart failure and coronary artery disease). Nonetheless, very little is known about the retinal vascular and structural changes in patients with atrial fibrillation (AF). Our study aims to characterize the microvasculature and structure of the retina in AF patients and explore their differences in different types of AF (paroxysmal and sustained AF).

Methods

This cross-sectional study was conducted at the Departments of Neurology and Cardiology in West China Hospital, Chengdu, China. Individuals aged 40 years or older with a diagnosis of AF were eligible for inclusion and underwent an evaluation and diagnosis confirmation before enrollment. Control individuals aged 40 years or older and without a history of AF, ocular abnormalities/disease, or any significant systemic illness were recruited. The retinal vascular and structural parameters were assessed using swept-source optical coherence tomography (SS-OCT)/SS-OCT angiography. Echocardiographic data of left atrium (LA) diameter were collected in patients with AF at the time of inclusion.

Results

A total of 242 eyes of 125 participants [71 men (56.8%); mean (SD) age, 61.98 (8.73) years] with AF and 219 eyes of 111 control participants [53 men (47.7%); mean (SD) age, 62.31 (6.47) years] were analyzed. In our AF cohort, 71 patients with paroxysmal AF and 54 patients with sustained AF (i.e., persistent/permanent AF) were included. Decreased retinal microvascular perfusion (β coefficient = −0.08; 95% CI, −0.14 to −0.03) and densities (β coefficient = −1.86; 95% CI, −3.11 to −0.60) in superficial vascular plexus (SVC) were found in the eyes of the participants with AF. In regard to retinal structures, thinner ganglion cell–inner plexiform layer (GCIPL; β coefficient = −2.34; 95% CI, −4.32 to −0.36) and retinal nerve fiber layer (RNFL) thicknesses (β coefficient = −0.63; 95% CI, −2.09 to −0.18) were observed in the eyes of the participants with AF. The retinal parameters did not significantly differ between paroxysmal and sustained AF (all P > 0.05). However, significant interactions were observed between LA diameter and AF subtypes with the perfusion and densities in SVC (P < 0.05).

Conclusion

This study found that individuals with AF had decreased retinal vascular densities and perfusion in SVC, as well as thinner GCIPL and RNFL thickness compared with age- and sex-matched control participants. The differences of the retinal microvasculature in SVC between paroxysmal and sustained AF depend on the LA diameter. Given our findings, further longitudinal studies with our participants are of interest to investigate the natural history of retinal microvascular and structural changes in individuals across the clinical process of AF and AF subtypes.
Hyperthermal-driven anoxia and reduced productivity in the aftermath of the Permian-Triassic mass extinction: a case study from Western Canada
Authors: Woods Adam D. , Zonneveld John-Paul , Wakefield Ryan
Published: December 2023

Introduction: The eruption of the Siberian Traps near the Permian-Triassic boundary (PTB) resulted in the rapid input of vast amounts of CO2 into the atmosphere and the subsequent development of a hothouse climate across much of the Early Triassic. The distribution of environmental stresses led to a complex recovery as survivors navigated high SSTs in shallow settings and an expanded OMZ that impinged upon the continental shelves. The Ursula Creek section of western British Columbia preserves a complete Lower-lowermost Middle Triassic sedimentary record of deep-water facies and provides a means to examine how offshore oceanic conditions varied along the western continental margin of Pangaea across the PTB and the entire Permian-Triassic recovery interval.

Methods: A total of 204 samples were collected from the uppermost Permian Fantasque Formation, the Griesbachian—Dienerian Grayling Formation and the Smithian—Anisian Toad Formation and analyzed for major, minor, and trace elements in addition to %TOC contents.

Results: Anoxic to euxinic conditions were persistent during deposition of most of the study interval based on lithologic indicators (finely-laminated siltstone that lacks trace fossils and a benthic fauna) and elevated Mo, U, and V enrichment factors (EFs). Ba, Cu, Ni, P, and Zn EFs indicate dampened productivity (EFs <1) across the much of the Early Triassic that was the result of weakened upwelling during a global hothouse interval. An increase in Cu, Ni, P, and Zn enrichment factors during the Anisian track a decline in global temperatures and the reestablishment of coastal upwelling as global thermal gradients and wind speeds increased.

Discussion: The results of this study point to the persistence of hyperthermal events and associated environmental stressors and underscores the urgency of curbing modern greenhouse gas emissions to prevent Earth from tipping into a hothouse state.
Chinese Expert Consensus on ovarian function and fertility preservation of cervical cancer in pregnancy (2023)
Authors: Ruan Xiangyan
Published: December 2023

Cervical cancer in pregnancy (CCIP) refers to cervical cancer diagnosed during pregnancy, the most common gynecological malignant tumor. Because of the special physiological changes of CCIP, although preserving ovarian function and fertility is very important, the methods are very limited. There is no guideline or consensus on the preservation methods of ovarian function and fertility in this special period. Therefore, the Committee of Fertility Protection and Preservation of China Association for the Promotion of Health Science and Technology, combined with the Chinese Society of Gynecological Endocrinology affiliated to the International Society of Gynecological Endocrinology, Society Endocrinology Branch of Beijing Institute of Obstetrics & Gynecology, combined with Society on Fertility Preservation affiliated with the Chinese Preventive Medicine Association, organized relevant experts from different disciplines to formulate this consensus, in order to guide ovarian function and fertility preservation of CCIP patients.
Wnt/β-catenin signaling activation promotes lipogenesis in the steatotic liver via physical mTOR interaction
Authors: Wang Kewei , Zhang Rong , Lehwald Nadja , Tao Guo-Zhong , Liu Bowen , Liu Bo , Koh Yangseok , Sylvester Karl G.
Published: December 2023

Background and aims

Wnt/β-catenin signaling plays an important role in regulating hepatic metabolism. This study is to explore the molecular mechanisms underlying the potential crosstalk between Wnt/β-catenin and mTOR signaling in hepatic steatosis.

Methods

Transgenic mice (overexpress Wnt1 in hepatocytes, Wnt+) mice and wild-type littermates were given high fat diet (HFD) for 12 weeks to induce hepatic steatosis. Mouse hepatocytes cells (AML12) and those transfected to cause constitutive β-catenin stabilization (S33Y) were treated with oleic acid for lipid accumulation.

Results

Wnt+ mice developed more hepatic steatosis in response to HFD. Immunoblot shows a significant increase in the expression of fatty acid synthesis-related genes (SREBP-1 and its downstream targets ACC, AceCS1, and FASN) and a decrease in fatty acid oxidation gene (MCAD) in Wnt+ mice livers under HFD. Wnt+ mice also revealed increased Akt signaling and its downstream target gene mTOR in response to HFD. In vitro, increased lipid accumulation was detected in S33Y cells in response to oleic acid compared to AML12 cells reinforcing the in vivo findings. mTOR inhibition by rapamycin led to a down-regulation of fatty acid synthesis in S33Y cells. In addition, β-catenin has a physical interaction with mTOR as verified by co-immunoprecipitation in hepatocytes.

Conclusions

Taken together, our results demonstrate that β-catenin stabilization through Wnt signaling serves a central role in lipid metabolism in the steatotic liver through up-regulation of fatty acid synthesis via Akt/mTOR signaling. These findings suggest hepatic Wnt signaling may represent a therapeutic strategy in hepatic steatosis.
Association between fasting stress hyperglycemia ratio and contrast-induced acute kidney injury in coronary angiography patients: a cross-sectional study
Authors: Shan Yu , Lin Maoning , Gu Fangfang , Ying Shuxin , Bao Xiaoyi , Zhu Qiongjun , Tao Yecheng , Chen Zhezhe , Li Duanbin , Zhang Wenbin , Fu Guosheng , Wang Min
Published: December 2023

Aims

Stress hyperglycemia ratio (SHR), an emerging indicator of critical illness, exhibits a significant association with adverse cardiovascular outcomes. The primary aim of this research endeavor is to evaluate the association between fasting SHR and contrast-induced acute kidney injury (CI-AKI).

Methods

This cross-sectional study comprised 3,137 patients who underwent coronary angiography (CAG) or percutaneous coronary intervention (PCI). The calculation of fasting SHR involved dividing the admission fasting blood glucose by the estimated mean glucose obtained from glycosylated hemoglobin. CI-AKI was assessed based on elevated serum creatinine (Scr) levels. To investigate the relationship between fasting SHR and the proportion of SCr elevation, piecewise linear regression analysis was conducted. Modified Poisson’s regression analysis was implemented to evaluate the correlation between fasting SHR and CI-AKI. Subgroup analysis and sensitivity analysis were conducted to explore result stability.

Results

Among the total population, 482 (15.4%) patients experienced CI-AKI. Piecewise linear regression analysis revealed significant associations between the proportion of SCr elevation and fasting SHR on both sides (≤ 0.8 and > 0.8) [β = -12.651, 95% CI (−23.281 to −2.022), P = 0.020; β = 8.274, 95% CI (4.176 to 12.372), P < 0.001]. The Modified Poisson’s regression analysis demonstrated a statistically significant correlation between both the lowest and highest levels of fasting SHR and an increased incidence of CI-AKI [(SHR < 0.7 vs. 0.7 ≤ SHR < 0.9) β = 1.828, 95% CI (1.345 to 2.486), P < 0.001; (SHR ≥ 1.3 vs. 0.7 ≤ SHR < 0.9) β = 2.896, 95% CI (2.087 to 4.019), P < 0.001], which was further validated through subgroup and sensitivity analyses.

Conclusion

In populations undergoing CAG or PCI, both lowest and highest levels of fasting SHR were significantly associated with an increased occurrence of CI-AKI.
Chronic low-grade inflammation and ovarian dysfunction in women with polycystic ovarian syndrome, endometriosis, and aging
Authors: Orisaka Makoto , Mizutani Tetsuya , Miyazaki Yumiko , Shirafuji Aya , Tamamura Chiyo , Fujita Masayuki , Tsuyoshi Hideaki , Yoshida Yoshio
Published: December 2023

The ovarian microenvironment is critical for follicular development and oocyte maturation. Maternal conditions, including polycystic ovary syndrome (PCOS), endometriosis, and aging, may compromise the ovarian microenvironment, follicular development, and oocyte quality. Chronic low-grade inflammation can induce oxidative stress and tissue fibrosis in the ovary. In PCOS, endometriosis, and aging, pro-inflammatory cytokine levels are often elevated in follicular fluids. In women with obesity and PCOS, hyperandrogenemia and insulin resistance induce ovarian chronic low-grade inflammation, thereby disrupting follicular development by increasing oxidative stress. In endometriosis, ovarian endometrioma-derived iron overload can induce chronic inflammation and oxidative stress, leading to ovarian ferroptosis and fibrosis. In inflammatory aging (inflammaging), senescent cells may secrete senescence-associated secretory phenotype factors, causing chronic inflammation and oxidative stress in the ovary. Therefore, controlling chronic low-grade inflammation and fibrosis in the ovary would present a novel therapeutic strategy for improving the follicular microenvironment and minimizing ovarian dysfunction.
Tracking SARS-CoV-2 seropositivity in rural communities using blood-fed mosquitoes: a proof-of-concept study
Authors: Krajacich Benjamin J. , Samaké Djibril , Dao Adama , Diallo Moussa , Sanogo Zana Lamissa , Yaro Alpha Seydou , Zeguime Amatigue , Poudiougo Josué , Cissé Kadiatou , Traoré Mamadou , dit Assitoun Alassane , Faiman Roy , Zaidi Irfan , Woodford John , Duffy Patrick E. , Lehmann Tovi
Published: December 2023

Background

The spread of SARS-CoV-2 cannot be well monitored and understood in areas without capacity for effective disease surveillance. Countries with a young population will have disproportionately large numbers of asymptomatic or pauci-symptomatic infections, further hindering detection of infection. Sero-surveillance on a country-wide scale by trained medical professionals may be limited in a resource-limited setting such as Mali. Novel ways of broadly sampling the human population in a non-invasive method would allow for large-scale surveillance at a reduced cost.

Approach

Here we evaluate the collection of naturally blood-fed mosquitoes to test for human anti-SARS-CoV-2 antibodies in the laboratory and at five field locations in Mali.

Results

Immunoglobulin-G antibodies to multiple SARS-CoV-2 antigens were readily detected in mosquito bloodmeals by bead-based immunoassay through at least 10 h after feeding [mean sensitivity of 0.92 (95% CI 0.78–1) and mean specificity of 0.98 (95% CI 0.88–1)], indicating that most blood-fed mosquitoes collected indoors during early morning hours (and likely to have fed the previous night) are viable samples for analysis. We found that reactivity to four SARS-CoV-2 antigens rose during the pandemic from pre-pandemic levels. The crude seropositivity of blood sampled via mosquitoes was 6.3% in October and November 2020 across all sites, and increased to 25.1% overall by February 2021, with the most urban site reaching 46.7%, consistent with independent venous blood-based sero-surveillance estimates.

Conclusions

We have demonstrated that using mosquito bloodmeals, country-wide sero-surveillance of human diseases (both vector-borne and non-vector-borne) is possible in areas where human-biting mosquitoes are common, offering an informative, cost-effective, and non-invasive sampling option.
Deciphering the puzzle: a case report of Tjalma syndrome (pseudo-pseudo Meigs' syndrome) with profoundly elevated CA-125 and pleural effusion
Authors: Tan Qing , Xu Li Feng , Yan Ting , Huang Cheng Hui , Tao Yi , Huang Wen Hui , Yu Shui Lian
Published: December 2023

Elevated CA-125 levels, polyserous effusions (such as pleural effusion, ascites, etc.) in young women with systemic lupus erythematosus (SLE) may signal pseudo-pseudo Meigs’ syndrome (PPMS), after excluding other causes. We describe a 32-year-old SLE patient with recurrent bilateral pleural effusions and unexplained hypercalcemia for 10 months. Extensive evaluations revealed no infections or tumors. Cytokine analysis showed elevated interleukin (IL) levels, especially IL-6 in pleural effusion. Treatment with immunosuppressive therapy resulted in reduced cancer antigen (CA) 125 levels and decreased effusion volume, demonstrating a positive response to intervention in this case of PPMS.
A systematic review on the anthropogenic stressors on sessile benthic mesophotic reef communities: implications for temperate reef management in Australia
Authors: Wong Rachel H. X. , Monk Jacquomo , Perkins Nicholas R. , Barrett Neville S.
Published: December 2023

Temperate rocky reefs are complex habitats that support high levels of biodiversity and productivity. However, the sessile benthic communities attached to these reefs are vulnerable to climate change and local human impacts due to their limited capacity to rapidly avoid unfavourable environments. In southeastern Australia, high latitude rocky reefs face increasing threats from rapid warming, surpassing the global average. Existing management efforts for these reefs rely heavily on research from tropical coral ecosystems and shallow temperate algal-dominated rocky reefs, where sufficient data allows for reliable predictions on the future state of the systems. Recent evidence from seabed mapping programs indicates that mesophotic reefs (~30-150 m depth) are equally or more extensive than shallow systems in temperate waters. They provide novel ecosystem functions and could potentially act as climate refuges for some components of shallow water communities that extend across depth strata. However, despite their importance, very little is known about the threats faced by non-algal sessile organisms in either shallow water or mesophotic systems in many parts of the world, including temperate Australia. This review examined the current state of knowledge regarding the direct impact of physical disturbances, such as extreme weather events, ocean warming and destructive fishing practices, on sessile mesophotic communities in general. We then used this to infer the likely drivers of change in temperate mesophotic systems. We found that Australia’s temperate mesophotic ecosystems were less likely to be impacted by fishing, anchoring and lost fishing gears compared to the northern hemisphere due to lower population density. Instead, we identified present and future pulsed warming events and severe storms as major threats to Australia’s mesophotic systems. This conclusion is based on the lack of evidence supporting the existence of depth refuges from climate events in the current literature and the potential implications of the predicted future increases in high wind-wave energy and warming extending into the mesophotic depths. Drawing from observations globally, changes in the distribution of growth forms, size or diversity of sponges may be useful indicators of heat stress and seabed disturbances in temperate mesophotic systems.
Scalable noninvasive amplicon-based precision sequencing (SNAPseq) for genetic diagnosis and screening of β-thalassemia and sickle cell disease using a next-generation sequencing platform
Authors: Gupta Pragya , Arvinden V. R. , Thakur Priya , Bhoyar Rahul C. , Saravanakumar Vinodh , Gottumukkala Narendra Varma , Goswami Sangam Giri , Nafiz Mehwish , Iyer Aditya Ramdas , Vignesh Harie , Soni Rajat , Bhargava Nupur , Gunda Padma , Jain Suman , Gupta Vivek , Sivasubbu Sridhar , Scaria Vinod , Ramalingam Sivaprakash
Published: December 2023

β-hemoglobinopathies such as β-thalassemia (BT) and Sickle cell disease (SCD) are inherited monogenic blood disorders with significant global burden. Hence, early and affordable diagnosis can alleviate morbidity and reduce mortality given the lack of effective cure. Currently, Sanger sequencing is considered to be the gold standard genetic test for BT and SCD, but it has a very low throughput requiring multiple amplicons and more sequencing reactions to cover the entire HBB gene. To address this, we have demonstrated an extraction-free single amplicon-based approach for screening the entire β-globin gene with clinical samples using Scalable noninvasive amplicon-based precision sequencing (SNAPseq) assay catalyzing with next-generation sequencing (NGS). We optimized the assay using noninvasive buccal swab samples and simple finger prick blood for direct amplification with crude lysates. SNAPseq demonstrates high sensitivity and specificity, having a 100% agreement with Sanger sequencing. Furthermore, to facilitate seamless reporting, we have created a much simpler automated pipeline with comprehensive resources for pathogenic mutations in BT and SCD through data integration after systematic classification of variants according to ACMG and AMP guidelines. To the best of our knowledge, this is the first report of the NGS-based high throughput SNAPseq approach for the detection of both BT and SCD in a single assay with high sensitivity in an automated pipeline.
Diffusion basis spectrum imaging detects subclinical traumatic optic neuropathy in a closed-head impact mouse model of traumatic brain injury
Authors: Yang Hsin-Chieh , Lavadi Raj Swaroop , Sauerbeck Andrew D. , Wallendorf Michael , Kummer Terrance T. , Song Sheng-Kwei , Lin Tsen-Hsuan
Published: December 2023

Introduction

Traumatic optic neuropathy (TON) is the optic nerve injury secondary to brain trauma leading to visual impairment and vision loss. Current clinical visual function assessments often fail to detect TON due to slow disease progression and clinically silent lesions resulting in potentially delayed or missed treatment in patients with traumatic brain injury (TBI).

Methods

Diffusion basis spectrum imaging (DBSI) is a novel imaging modality that can potentially fill this diagnostic gap. Twenty-two, 16-week-old, male mice were equally divided into a sham or TBI (induced by moderate Closed-Head Impact Model of Engineered Rotational Acceleration device) group. Briefly, mice were anesthetized with isoflurane (5% for 2.5 min followed by 2.5% maintenance during injury induction), had a helmet placed over the head, and were placed in a holder prior to a 2.1-joule impact. Serial visual acuity (VA) assessments, using the Virtual Optometry System, and DBSI scans were performed in both groups of mice. Immunohistochemistry (IHC) and histological analysis of optic nerves was also performed after in vivo MRI.

Results

VA of the TBI mice showed unilateral or bilateral impairment. DBSI of the optic nerves exhibited bilateral involvement. IHC results of the optic nerves revealed axonal loss, myelin injury, axonal injury, and increased cellularity in the optic nerves of the TBI mice. Increased DBSI axon volume, decreased DBSI λ||, and elevated DBSI restricted fraction correlated with decreased SMI-312, decreased SMI-31, and increased DAPI density, respectively, suggesting that DBSI can detect coexisting pathologies in the optic nerves of TBI mice.

Conclusion

DBSI provides an imaging modality capable of detecting subclinical changes of indirect TON in TBI mice.
Probing the role of the C2F domain of otoferlin
Authors: Chen Han , Fang Qinghua , Benseler Fritz , Brose Nils , Moser Tobias
Published: December 2023

Afferent synapses of cochlear inner hair cells (IHCs) employ a unique molecular machinery. Otoferlin is a key player in this machinery, and its genetic defects cause human auditory synaptopathy. We employed site-directed mutagenesis in mice to investigate the role of Ca2+ binding to the C2F domain of otoferlin. Substituting two aspartate residues of the C2F top loops, which are thought to coordinate Ca2+-ions, by alanines (OtofD1841/1842A) abolished Ca2+-influx-triggered IHC exocytosis and synchronous signaling in the auditory pathway despite substantial expression (~60%) of the mutant otoferlin in the basolateral IHC pole. Ca2+ influx of IHCs and their resting membrane capacitance, reflecting IHC size, as well as the number of IHC synapses were maintained. The mutant otoferlin showed a strong apex-to-base abundance gradient in IHCs, suggesting impaired protein targeting. Our results indicate a role of the C2F domain in otoferlin targeting and of Ca2+ binding by the C2F domain for IHC exocytosis and hearing.
TICRR serves as a prognostic biomarker in lung adenocarcinoma with implications in RNA epigenetic modification, DDR pathway, and RNA metabolism
Authors: Zheng Xunbo , Han Li , Guan Jun , Chen Chenteng , Zhang Yue , Zhang Jiali , Zhang Yiran , Liu Siyao , Su Junyan , Liu Mengyuan , Huang Hanxing
Published: December 2023

Purpose

TOPBP1 interacting checkpoint and replication regulator (TICRR), a hub gene of the Cdk2-mediated initiation step of DNA replication, has been shown an essential role in tumorigenesis by accelerating the DNA replication of tumor cells.

Methods

RT-qPCR was used to detect the mRNA expression of TICRR in LUAD tumors and adjacent normal tissues. The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) database of LUAD were acquired to analyze the critical role of TICRR expression in survival prognosis and clinicopathology characters in LUAD. Gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and gene set enrichment analysis (GSEA) were performed using the R package. The correlation of TICRR expression with immune cell infiltration, RNA epigenetic modification, DNA damage repair (DDR) pathway, and cell metabolism of LUAD was further explored to verify significant conclusions.

Results

TICRR was significantly upregulated in most cancer types, including LUAD, lung squamous cell carcinoma (LUSC), and others. Cox regression analysis indicated the overexpression of TICRR was associated with poor survival in several cancers. In LUAD, TICRR expression was positively correlated with tumor stage and was increased in smoking, male, and high tumor mutational burden (TMB) patients. Enrichment analysis revealed that TICRR could influence tumor proliferation and prognosis via activating pathways involving cell cycle, DNA repair, DNA replication, cysteine metabolism, oxidative phosphorylation, and ubiquitin-mediated proteolysis pathways. Interestingly, high TICRR expression correlated with DDR pathway signature (34 genes), 37 m6A/m5C regulated genes, and some metabolism-regulated genes. Silencing the TICRR gene affects cysteine metabolism and modifies cancer-related pathways, with decreased cell cycle and increased B/T cell receptor signaling. Our TICRR risk model accurately predicts LUAD patient prognosis, validated across GEO datasets, and is integrated with clinical characteristics via a nomogram, facilitating personalized treatment strategies and enhancing patient management.

Conclusions

Taken together, TICRR has emerged as a promising prognostic biomarker in lung adenocarcinoma (LUAD), with implications in immune activation, cell cycle regulation, RNA modification, and tumor energy metabolism. These findings suggest that TICRR could serve as a viable therapeutic target and a reliable prognostic indicator for LUAD.
Mechanical characteristics of graded origami bellows under axial tension
Authors: Zhang Xinyi , Lu Guoxing , Wang Shenghai , Durandet Yvonne
Published: December 2023

In this study, the mechanical characteristics of the graded origami bellows were numerically investigated and experimentally validated. Two graded geometric parameters were considered: pre-folding angle (θ) and layer height (Le). The sensitivities of the deployment process and energy absorption efficiency of origami bellows to variations in θ and Le under quasi-static loading and dynamic loading were numerically investigated. Results show that the origami bellows with positive gradients exhibited progressive deployment process. More than one deformation mechanism was triggered during deployment, indicating a mixed non-rigid deployment mode. A large gradient of θ had a notable effect on the energy absorption efficiency. Both specific energy absorption (SEA) and mean tensile force (Pm) decreased as the gradient of θ increased. Although the gradient of Le was insensitive to the force response, the SEA decreased as the gradient of Le increased. The dynamic behavior of the graded models indicated that both the initial peak force and SEA were affected by the graded parameters. In general, the proposed graded origami bellows show a controllable deployment process and a stable force response under axial tension.
Increasing planting density can improve the yield of Tartary buckwheat
Authors: Zhou Qiuyue , He Peiyun , Tang Jingang , Huang Kaifeng , Huang Xiaoyan
Published: December 2023

Planting densities and nitrogen fertilizer application rates determine the yield of crops. Tartary buckwheat is a pseudocereal crop with great health care and development values. However, little is known about application of nitrogen fertilizer and planting density on the physiological characteristics of Tartary buckwheat. This study aims to clarify the effect of planting density on the senescence and yield of Tartary buckwheat under low nitrogen conditions. A 2-year field experiment was conducted on Tartary buckwheat (Jinqiao 2) to study the effects of different planting densities (8 × 105, 10 × 105, 12 × 105, 14 × 105, and 16 × 105 plants·ha−1) on the root morphology and activity, chlorophyll and malondialdehyde (MDA) contents, antioxidant enzyme activity, photosynthetic characteristics, agronomic traits, and yield of Tartary buckwheat in the absence of nitrogen fertilizer treatment. With the increase in planting density, the root morphological indices and activities; chlorophyll a, chlorophyll b, and carotenoid contents; superoxide dismutase and peroxidase activities; net photosynthetic rate; transpiration rate; intercellular CO2 concentration and transpiration rate; main stem node, branch, and leaf numbers; grain number and weight per plant; and 1000-grain weight of Jinqiao 2 decreased continuously, whereas plant height and leaf MDA content increased continuously. The yield of Tartary buckwheat first increased and then decreased with the increase in planting density. The yield under 14 × 105 plants·ha−1 treatment increased by 68.61%, 44.82%, 11.00%, and 22.36%, respectively, relative to that under 8 × 105, 10 × 105, 12 × 105, and 16 × 105 plants·ha−1treatments. In summary, planting at an appropriately high density (14 × 105 plants·ha−1) can promote the increase in the yield of Tartary buckwheat populations under low nitrogen conditions and is recommended for use in production to achieve the high-yielding and nitrogen saving cultivation of Tartary buckwheat. This research can serve as a theoretical basis to jointly achieve the high yield and nitrogen saving of Tartary buckwheat.
Interactions among national and supranational identities: mobilizing the independence movement in Scotland
Authors: Heaney Michael T. , Anderson Erika L. , Cancro Madilyn E. , Martin Grace E.
Published: December 2023

As nationalism rises worldwide, understanding the relevance of national identities is at a premium in both the study of mass political behavior and the analysis of social movements. Drawing on research in social psychology, this study exploresinteractionsamong national and supranational identities using the concepts ofidentity interference(i.e., negative interactions) andidentity complementarity(i.e., positive interactions). These interactions extend beyond thedirecteffects of identity considered in many previous studies. Focusing on interactions centers the analysis on the contextual aspects of identity during nationalist mobilizations. Survey data from Scotland demonstrate that interactions among Scottish, British, and European identities were consequential for mobilizing support for Scottish independence in 2019. Strong evidence indicates interference between Scottish and British identities. European and Scottish identities complement one another among independence supporters but not in the general population. The possibility of interference between European and British identities is backed by only mixed results. The timing of this study in the aftermath of the Brexit referendum was likely relevant to its findings on European identity. Overall, this research illustrates the benefits of widening the empirical examination of multiple identities in the social sciences.
Advances in the prevention and treatment of Alzheimer’s disease based on oral bacteria
Authors: Zhang Miaomiao , Mi Nannan , Ying Zheng , Lin Xiaoping , Jin Ying
Published: December 2023

With the global population undergoing demographic shift towards aging, the prevalence of Alzheimer’s disease (AD), a prominent neurodegenerative disorder that primarily afflicts individuals aged 65 and above, has increased across various geographical regions. This phenomenon is accompanied by a concomitant decline in immune functionality and oral hygiene capacity among the elderly, precipitating compromised oral functionality and an augmented burden of dental plaque. Accordingly, oral afflictions, including dental caries and periodontal disease, manifest with frequency among the geriatric population worldwide. Recent scientific investigations have unveiled the potential role of oral bacteria in instigating both local and systemic chronic inflammation, thereby delineating a putative nexus between oral health and the genesis and progression of AD. They further proposed the oral microbiome as a potentially modifiable risk factor in AD development, although the precise pathological mechanisms and degree of association have yet to be fully elucidated. This review summarizes current research on the relationship between oral bacteria and AD, describing the epidemiological and pathological mechanisms that may potentially link them. The purpose is to enrich early diagnostic approaches by incorporating emerging biomarkers, offering novel insights for clinicians in the early detection of AD. Additionally, it explores the potential of vaccination strategies and guidance for clinical pharmacotherapy. It proposes the development of maintenance measures specifically targeting oral health in older adults and advocates for guiding elderly patients in adopting healthy lifestyle habits, ultimately aiming to indirectly mitigate the progression of AD while promoting oral health in the elderly.
Carbon dots-based fluorescent films to act as a potential antioxidant agent and pH ratiometric sensor for skin applications
Authors: Da Silva Livia E. , Araujo Anna Paula de L. , Almeida James Henrique , de Vasconcelos Ledja. B. O. D. , Silva Messias de O. , Lima Dimas. J. P. , Viana Rodrigo da S. , Ferro Jamylle N. de S. , Goulart Marília O. F. , Xavier Jadriane A. , Barbosa Cintya. D. A. E. S.
Published: December 2023

The wound healing process is accompanied by changes in pH values. Monitoring this physicochemical parameter can indicate the effectiveness of the applied treatment and act as early identification of wound infection. This study focuses on the development of a fluorescent film-based polyvinyl alcohol (PVA) and carbon dots (CDs) derived from lemon bagasse (CD-L) and ortho-phenylenediamine (CD-oPD) named to act as antioxidants and potential ratiometric fluorescent pH sensors, in wound applications. The I460/I550 intensity ratio, as a function of pH value for the dual-system CDs prepared from the mixture of CD-L and CD-oPD, named CD-L/oPD, was investigated. The fit corresponded to a sigmoidal function in the pH range of 5–10, with a relationship having a r2 = 0.992. The variation in the values of the I460/I550 ratio allows for the visualization of the color change from yellowish-green to green with increasing pH. Through a simulated ex vivo pig skin model, it was possible to note that the films prepared from mixed of the CD-L and CD-oPD carbon dots incorporated in a matrix PVA named CD-L/oPD-F was more efficient at visually discriminating color in relation to changes in pH than the films prepared from both individual CD-L (CD-L-F) and CD-oPD (CD-oPD-F) carbon dots. CD-L and CD-oPD demonstrated antioxidant capacity against reactive oxygen species (ROS). The IC50 values for CD-L and CD-oPD were 56.7 and 39.5 μg mL−1 in the DPPH● inhibition assay, and 25.1 and 63.4 μg mL−1 in the HOCl scavenging one, respectively. MTT viability assays using human non-tumoral skin fibroblast (HFF-1) cell showed a cell survival rate of over 80% for both CDs up to a concentration of 1,000 μg mL−1. Finally, the developed films can act in a bifunctional way, by monitoring healing through pH changes and by acting as an antioxidant agent in the treatment of wounds.
Investigating machine learning and ensemble learning models in groundwater potential mapping in arid region: case study from Tan-Tan water-scarce region, Morocco
Authors: Jari Abdessamad , Bachaoui El Mostafa , Hajaj Soufiane , Khaddari Achraf , Khandouch Younes , El Harti Abderrazak , Jellouli Amine , Namous Mustapha
Published: December 2023

Groundwater resource management in arid regions has a critical importance for sustaining human activities and ecological systems. Accurate mapping of groundwater potential plays a vital role in effective water resource planning. This study investigates the effectiveness of machine learning models, including Random Forest (RF), Adaboost, K-Nearest Neighbors (KNN), and Gaussian Process in groundwater potential mapping (GWPM) in the Tan-Tan arid region, Morocco. Fourteen groundwater conditional factors were considered following multicollinearity test, including topographical, hydrological, climatic, and geological factors. Additionally, point data with 174 sites indicative of groundwater occurrences were incorporated. The groundwater inventory data underwent random partitioning into training and testing datasets at three different ratios: 55/45%, 65/35%, and 75/25%. Ultimately, a comprehensive ranking of the 13 models, encompassing both individual and ensemble models, was determined using the prioritization rank technique. The results revealed that ensemble learning (EL) models, particularly RF and Adaboost (RF-Adaboost), outperformed individual models in groundwater potential mapping. Based on accuracy assessment using the validation dataset, the RF-Adaboost EL results yielded an Area Under the Receiver Operating characteristic Curve (AUROC) and Overall Accuracy (OA) of 94.02 and 94%, respectively. Ensemble models have been effectively applied to integrate 14 factors, capturing their intricate interrelationships, and thereby enhancing the accuracy and robustness of groundwater prediction in the Tan-Tan water-scarce region. Among the natural factors, the current study identified lithology, structural elements (such as faults and tectonic lineaments), and land use as significant contributors to groundwater potential. However, the critical characteristics of the study area showing a coastal position as well as a low background in groundwater prospectivity (low borehole points) are challenging in GWPM. The findings highlight the importance of the significant factors in assessing and managing groundwater resources in arid regions. Moreover, this study makes a contribution to the management of groundwater resources by demonstrating the effectiveness of ensemble learning algorithms in the groundwater potential mapping (GWPM) in arid regions.
Implementation of an automated process for Limnospira indica harvesting and culture medium recycling for space applications
Authors: Tallec Jordan , Vandermies Marie , Coene Céline , Lamaze-Lefebvre Brigitte , Demey Dries , Frappart Matthieu , Couallier Estelle
Published: December 2023

Future long-term space exploration missions require the implementation of circular life support systems for the supply of water, oxygen and food from mission wastes. Therefore, separation systems dealing with multi-phasic streams need to be addressed. The BioHarvest (BHV) study focused on solid/liquid separation in space with the aim to demonstrate the continuous separation and harvesting of the cyanobacterium Limnospira indica from its culture broth under axenic conditions. The cyanobacterium biomass is intended to be used for further food processing while the broth free of organic matter and resupplied with nutrients should be directly recycled into the photobioreactor (PBR). In this study, an automated breadboard model based on a two-step process was built. First, a Biomass Harvesting Unit (BHU) separates the biomass produced in the PBR from the culture medium with dead-end filtration. Second, the Medium Filtration Unit (MFU) further treats the culture medium to retain the dissolved organic compounds using crossflow filtration. The performances of BHU and MHU met the requirements in batch mode and in short continuous mode: the BHU was able to retain all the biomass and the MFU could retain more than 90% of organic matter while being permeable to nutrients. The productivity of the MFU was also very good, with a high permeation flux allowing treating the targeted 80 L of culture per day. However, continuous operation of the BHV technology could not be achieved in the long term due to biomass accumulation as a sticky cake with a high specific resistance on the BHU filter, despite backwashing cycles and intense vibrations. Future work shall therefore focus on this critical step, to improve process performance by preventing fouling of the filter sheets.
Boar Seminal Microbiota in Relation to Sperm Quality under Tropical Environments
Authors: Ngo CongBang , Suwimonteerabutr Junpen , Apiwatsiri Prasert , Saenkankam Imporn , Prapasarakul Nuvee , Morrell Jane M. , Tummaruk Padet
Published: December 2023

The present study was carried out to determine the seminal microbiota of boars and their correlation with sperm quality. A total of 17 ejaculates were collected from 17 Duroc boars and were classified according to sperm quality into two groups: low-quality (n = 8) and high-quality (n = 9). Each ejaculate was subjected to (i) semen evaluation, (ii) bacterial culture and MALDI-TOF identification, and (iii) 16S rRNA gene sequencing and bioinformatic analyses. No difference in the total bacterial count, alpha diversity, and beta diversity between the high-quality group and the low-quality group was detected (p > 0.05). While Globicatella sanguinis was negatively correlated with sperm quality (p < 0.05), Delftia acidovorans was positively correlated with sperm quality (p < 0.05). Lactobacillales (25.2%; LB) and Enterobacterales (10.3%; EB) were the most dominant bacteria and negatively correlated: EB = 507.3 − 0.5 × LB, R2 = 0.24, p < 0.001. Moreover, the abundance of Escherichia-shigella was negatively correlated with LB (r = −0.754, p < 0.001) and positively correlated with Proteus (r = 0.533, p < 0.05). Alysiella was positively correlated with Lactobacillus (r = 0.485, p < 0.05), Prevotella (r = 0.622, p < 0.01), and Staphylococcus (r = 0.489, p < 0.05). In conclusion, seminal microbiota is significantly associated with boar semen qualities. The distributions of the most dominant bacterial genera, the differences in the abundance of small subset microbes, and their correlation appear to have far more impact than the overall seminal bacterial content (e.g., total bacterial count, alpha diversity, and beta diversity) on sperm quality.
Characterization of the Complete Mitochondrial Genome of the Elongate Loach and Its Phylogenetic Implications in Cobitidae
Authors: Ke Zhenlin , Zhou Kangqi , Hou Mengdan , Luo Hui , Li Zhe , Pan Xianhui , Zhou Jian , Jing Tingsen , Ye Hua
Published: December 2023

The elongate loach is an endemic fish in China. Previous studies have provided some insights into the mitochondrial genome composition and the phylogenetic relationships of the elongate loach inferred using protein-coding genes (PCGs), yet detailed information about it remains limited. Therefore, in this study we sequenced the complete mitochondrial genome of the elongate loach and analyzed its structural characteristics. The PCGs and mitochondrial genome were used for selective stress analysis and genomic comparative analysis. The complete mitochondrial genome of the elongate loach, together with those of 35 Cyprinidae species, was used to infer the phylogenetic relationships of the Cobitidae family through maximum likelihood (ML) reconstruction. The results showed that the genome sequence has a full length of 16,591 bp, which includes 13 PCGs, 22 transfer RNA genes (tRNA), 2 ribosomal RNA genes (rRNA), and 2 non-coding regions (CR D-loop and light chain sub-chain replication origin OL). Overall, the elongate loach shared the same gene arrangement and composition of the mitochondrial genes with other teleost fishes. The Ka/Ks ratios of all mitochondrial PCGs were less than 1, indicating that all of the PCGs were evolving under purifying selection. Genome comparison analyses showed a significant sequence homology of species of Leptobotia. A significant identity between L. elongata and the other five Leptobotia species was observed in the visualization result, except for L. mantschurica, which lacked the tRNA-Arg gene and had a shorter tRNA-Asp gene. The phylogenetic tree revealed that the Cobitidae species examined here can be grouped into two clades, with the elongate loach forming a sister relationship with L. microphthalma. This study could provide additional inferences for a better understanding of the phylogenetic relationships among Cobitidae species.
Characterization of Inflammasomes and Their Regulation in the Red Fox
Authors: Ahn Huijeong , Jeong Dong-Hyuk , Lee Gilyoung , Lee Suk-Jin , Yang Jeong-Jin , Kim Yo-Han , Hahn Tae-Wook , Choi Sooyoung , Lee Geun-Shik
Published: December 2023

Background: Inflammasomes recognize endogenous and exogenous danger signals, and subsequently induce the secretion of IL-1β. Studying inflammasomes in the red fox (Vulpes vulpes) is crucial for wildlife veterinary medicine, as it can help control inflammatory diseases in foxes. Methods: We investigated the activation and intracellular mechanisms of three inflammasomes (NLRP3, AIM2, and NLRC4) in fox peripheral blood mononuclear cells (PBMCs), using established triggers and inhibitors derived from humans and mice. Results: Fox PBMCs exhibited normal activation and induction of IL-1β secretion in response to representative inflammasome triggers (ATP and nigericin for NLRP3, dsDNA for AIM2, flagellin for NLRC4). Additionally, PBMCs showed normal IL-1β secretion when inoculated with inflammasome-activating bacteria. In inhibitors of the inflammasome signaling pathway, fox inflammasome activation was compared with mouse inflammasomes. MCC950, a selective NLRP3 inhibitor, suppressed the secretion of dsDNA- and flagellin-mediated IL-1β in foxes, unlike mice. Conclusions: These findings suggest that NLRP3 may have a common role in dsDNA- and flagellin-mediated inflammasome activation in the red fox. It implies that this fox inflammasome biology can be applied to the treatment of inflammasome-mediated diseases in the red fox.