bluuebunny/crossref_metadata_2025 huggingface dataset:
Deletion of RasGRF1 Attenuated Interstitial Fibrosis in Streptozotocin-Induced Diabetic Cardiomyopathy in Mice through Affecting Inflammation and Oxidative Stress
Authors: Tsai Tzu-Hsien , Lin Cheng-Jei , Chua Sarah , Chung Sheng-Ying , Chen Shyh-Ming , Lee Chien-Ho , Hang Chi-Ling
Published: October 2018

Background: Diabetic cardiomyopathy (DCM) is characterized by cardiac fibrosis and stiffness, which often develops into heart failure. This study investigated the role of Ras protein-specific guanine nucleotide releasing factor 1 (RasGRF1) in the development of DCM. Methods: Forty-eight mice were divided into four groups (n = 12 per group): Group 1: Wild-type (WT) mice, Group 2: RasGRF1 deficiency (RasGRF1−/−) mice. Group 3: Streptozotocin (STZ)-induced diabetic WT mice, Group 4: STZ-induced diabetic RasGRF1−/− mice. Myocardial functions were assessed by cardiac echography. Heart tissues from all of the mice were investigated for cardiac fibrosis, inflammation, and oxidative stress markers. Results: Worse impaired diastolic function with elevation serum interleukin (IL)-6 was found in the diabetic group compared with the non-diabetic groups. Serum IL-6 levels were found to be elevated in the diabetic compared with the non-diabetic groups. However, the diabetic RasGRF1−/− mice exhibited lower serum IL-6 levels and better diastolic function than the diabetic WT mice. The diabetic RasGRF1−/− mice were associated with reduced cardiac inflammation, which was shown by lower invading inflammation cells, lower expression of matrix metalloproteinase 9, and less chemokines compared to the diabetic WT mice. Furthermore, less oxidative stress as well as extracellular matrix deposition leading to a reduction in cardiac fibrosis was also found in the diabetic RasGRF1−/− mice compared with the diabetic WT mice. Conclusion: The deletion of RasGRF1 attenuated myocardial fibrosis and improved cardiac function in diabetic mice through inhibiting inflammation and oxidative stress.
Solvent and Substituent Effects on the Phosphine + CO2 Reaction
Authors: Alkorta Ibon , Trujillo Cristina , Sánchez-Sanz Goar , Elguero José
Published: October 2018

A theoretical study of the substituent and solvent effects on the reaction of phosphines with CO2 has been carried out by means of Møller-Plesset (MP2) computational level calculations and continuum polarizable method (PCM) solvent models. Three stationary points along the reaction coordinate have been characterized, a pre-transition state (TS) assembly in which a pnicogen bond or tetrel bond is established between the phosphine and the CO2 molecule, followed by a transition state, and leading finally to the adduct in which the P–C bond has been formed. The solvent effects on the stability and geometry of the stationary points are different. Thus, the pnicogen bonded complexes are destabilized as the dielectric constant of the solvent increases while the opposite happens within the adducts with the P–C bond and the TSs trend. A combination of the substituents and solvents can be used to control the most stable minimum.
Sea Urchins as an Inspiration for Robotic Designs
Authors: Stiefel Klaus M. , Barrett Glyn A.
Published: October 2018

Neuromorphic engineering is the approach to intelligent machine design inspired by nature. Here, we outline possible robotic design principles derived from the neural and motor systems of sea urchins (Echinoida). Firstly, we review the neurobiology and locomotor systems of sea urchins, with a comparative emphasis on differences to animals with a more centralized nervous system. We discuss the functioning and enervation of the tube feet, pedicellariae, and spines, including the limited autonomy of these structures. We outline the design principles behind the sea urchin nervous system. We discuss the current approaches of adapting these principles to robotics, such as sucker-like structures inspired by tube feet and a robotic adaptation of the sea urchin jaw, as well as future directions and possible limitations to using these principles in robots.
An Ultrasonication-Assisted Cobalt Hydroxide Composite with Enhanced Electrocatalytic Activity toward Oxygen Evolution Reaction
Authors: Si Yujun , Guo Chaozhong , Xie Chenglong , Xiong Zhongping
Published: October 2018

A catalyst toward oxygen evolution reaction (OER) was synthesized by depositing cobalt hydroxide on carbon black. Ultrasonication was applied during precipitation to improve the performance of the catalyst. The ultrasonic-assisted process resulted in the refinement of the cobalt hydroxide particles from 400 nm to 50 nm, and the thorough incorporation of these particles with carbon black substrate. The resulting product exhibited enhanced OER catalytic activity with an onset potential of 1.54 V (vs. reversible hydrogen electrode), a Tafel slope of 18.18 mV/dec, and a stable OER potential at a current density of 10 mA cm−2, because of the reduced resistance of the catalyst and the electron transfer resistance.
Preparation of Parabolic Superhydrophobic Material for Oil-Water Separation
Authors: Qiao Xiaoying , Yang Chunyan , Zhang Qian , Yang Shengke , Chen Yangyang , Zhang Dan , Yuan Xiaoyu , Wang Wenke , Zhao Yaqian
Published: October 2018

In order to prepare parabolic superhydrophobic materials, copper meshes were used as the substrate and ultrasonic etching and oxidative corrosion were carried out with FeCl3 solution and H2O2 solution, respectively, and then the surface was modified with stearic acid (SA). The topological structure and surface wettability of the prepared mesh were characterized by fluorescence microscope, scanning electron microscopy and contact angle measurement. Finally, the as-prepared copper meshes were applied to oil-water separation. The results showed that the micro-nano-mastoid structure on the surface of the copper mesh was flaky bulges, forming a rough structure similar to a paraboloid. When the oxidative corrosion time of H2O2 was 1 min, it is more beneficial to increase the hydrophobicity of the surface of the copper mesh and increase the contact angle of water droplets on the surface of the membrane. Additionally, based on superhydrophobic materials of the parabolic copper mesh, the static contact angles of the water droplets, engine oil and carbon tetrachloride with the surface were approximately 153.6°, 5° and 0.1°, respectively and the sliding angle of the water droplets with the surface were approximately 4.9°. The parabolic membrane was applied to discuss the separation efficiency of different oils with deionized water and the separation efficiency was obtained as benzene > carbon tetrachloride > oil > machine oil. Therefore, based on the research, the parabolic superhydrophobic material has good efficiency of oil-water separation.
Risk Management of Hazardous Materials in Manufacturing Processes: Links and Transitional Spaces between Occupational Accidents and Major Accidents
Authors: Brocal Francisco , González Cristina , Reniers Genserik , Cozzani Valerio , Sebastián Miguel A.
Published: October 2018

Manufacturing processes involving chemical agents are evolving at great speed. In this context, managing chemical risk is especially important towards preventing both occupational accidents and major accidents. Directive 89/391/EEC and Directive 2012/18/EU, respectively, are enforced in the European Union (EU) to this end. These directives may be further complemented by the recent ISO 45001:2018 standard regarding occupational health and safety management systems. These three management systems are closely related. However, scientific literature tackles the researching of these accidents independently. Thus, the main objective of this work is to identify and analyse the links and transitional spaces between the risk management of both types of accident. Among the results obtained, three transitional spaces can be pointed out which result from the intersection of the three systems mentioned. Similarly, the intersection of these spaces gives shape to a specific transitional space defined by the individual directives linked to Directive 89/391/EEC. These results are limited from a regulatory and technical perspective. Thus, the results are a starting point towards developing models that integrate the management systems studied.
Application of Differential Entropy in Characterizing the Deformation Inhomogeneity and Life Prediction of Low-Cycle Fatigue of Metals
Authors: Zhang Mu-Hang , Shen Xiao-Hong , He Lei , Zhang Ke-Shi
Published: October 2018

The relation between deformation inhomogeneity and low-cycle-fatigue failure of T2 pure copper and the nickel-based superalloy GH4169 under symmetric tension-compression cyclic strain loading is investigated by using a polycrystal representative volume element (RVE) as the material model. The anisotropic behavior of grains and the strain fields are calculated by crystal plasticity, taking the Bauschinger effect into account to track the process of strain cycles of metals, and the Shannon’s differential entropies of both distributions of the strain in the loading direction and the first principal strain are employed at the tension peak of the cycles as measuring parameters of strain inhomogeneity. Both parameters are found to increase in value with increments in the number of cycles and they have critical values for predicting the material’s fatigue failure. Compared to the fatigue test data, it is verified that both parameters measured by Shannon’s differential entropies can be used as fatigue indicating parameters (FIPs) to predict the low cycle fatigue life of metal.
Fatigue Damage Evaluation Using Nonlinear Lamb Waves with Quasi Phase-Velocity Matching at Low Frequency
Authors: Zhu Wujun , Xiang Yanxun , Liu Chang-jun , Deng Mingxi , Ma Congyun , Xuan Fu-zhen
Published: October 2018

Due to the dispersive and multimode natures, only nonlinear Lamb waves with exact phase-velocity matching were generally used in previous studies to evaluate the evenly distributed microstructural evolution in the incipient stage of material degradation, because of the cumulative generation of second harmonics, which was also found within a significant propagation distance for mode pair S0-s0 with quasi phase-velocity matching at low frequency. To explore the feasibility of fatigue damage evaluation by using this mode pair and fully utilize its unique merits, the cumulative second harmonic analysis was performed on aluminum alloy specimens with various material damage produced by the continuous low cycle fatigue tests. Similar to mode pair S1-s2 with exact phase-velocity matching, a mountain shape curve between the normalized acoustic nonlinearity parameter and the fatigue life was also achieved with the peak point at about 0.65 fatigue life for mode pair S0-s0, even though a relatively higher sensitivity to fatigue damage was observed for mode pair S1-s2. The excited frequency selection was further analyzed in a certain frequency range, where the quasi phase-velocity matching condition was satisfied for mode pair S0-s0 owing to the less dispersive property. Results show that the fatigue damage can be effectively detected using the mode pair S0-s0, and a relatively lower excited frequency was preferred due to its higher sensitivity to microstructural evolution.
A Novel Approach for Assessing the Fatigue Behavior of PEEK in a Physiologically Relevant Environment
Authors: Peron Mirco , Torgersen Jan , Berto Filippo
Published: October 2018

In recent years, the need of surgical procedures has continuously increased and, therefore, researchers and clinicians are broadly focusing on the development of new biocompatible materials. Among them, polyetheretherketone (PEEK) has gained wide interest in load-bearing applications due to its yielding behaviour and its superior corrosion resistance. To assure its reliability in these applications where notches and other stress concentrators weaken implants resistance, a design tool for assessing its tensile and fatigue behaviour in the presence of geometrical discontinuities is highly claimed. Herein, a new fatigue design method based on a local approach is proposed for PEEK implant, and the results are compared with those obtained using the two main biomaterial design approaches available in literature, i.e., the theory of critical distances (TCD) and the notch stress intensity factor (NSIF) approach. To this aim, previously published datasets of PEEK-notched specimens are used, and the proposed method is reported to provide more accurate results and to be robust for different notch geometries.
On the Improvement of Thermal Protection for Temperature-Responsive Protective Clothing Incorporated with Shape Memory Alloy
Authors: He Jiazhen , Lu Yehu , Wang Lijun , Ma Nini
Published: October 2018

This study explored the application of shape memory alloy (SMA) springs in a multilayer protective fabric assembly for intelligent insulation that responded to thermal environment changes. Once the SMA spring was actuated, clothing layers were separated, creating an adjustable air gap between the adjacent fabric layers. The impacts of six different SMA arrangement modes and two different spring sizes on thermal protection against either a radiant heat exposure (12 kW/m2) or a hot surface exposure (400 °C) were investigated. The findings showed that the incorporation of SMA springs into the fabric assembly improved the thermal protection, but the extent to which the springs provided thermal protection was dependent on the arrangement mode and spring size. The effectiveness of reinforcing the protective performance using SMA springs depended on the ability of clothing layers to expand an air layer. The regression models were established to quantitatively assess the relationship between the air gap formed by SMA spring and the thermal protective performance of clothing. This study demonstrated the potential of SMA spring as a suitable material for the development of intelligent garments to provide additional thermal protection and thus reduce the number of clothing layers for transitional thermal protective clothing.
Fucoxanthin-Containing Cream Prevents Epidermal Hyperplasia and UVB-Induced Skin Erythema in Mice
Authors: Rodríguez-Luna Azahara , Ávila-Román Javier , González-Rodríguez María Luisa , Cózar María José , Rabasco Antonio M , Motilva Virginia , Talero Elena
Published: October 2018

Microalgae represent a source of bio-active compounds such as carotenoids with potent anti-inflammatory and antioxidant properties. We aimed to investigate the effects of fucoxanthin (FX) in both in vitro and in vivo skin models. Firstly, its anti-inflammatory activity was evaluated in LPS-stimulated THP-1 macrophages and TNF-α-stimulated HaCaT keratinocytes, and its antioxidant activity in UVB-irradiated HaCaT cells. Next, in vitro and ex vivo permeation studies were developed to determine the most suitable formulation for in vivo FX topical application. Then, we evaluated the effects of a FX-containing cream on TPA-induced epidermal hyperplasia in mice, as well as on UVB-induced acute erythema in hairless mice. Our results confirmed the in vitro reduction of TNF-α, IL-6, ROS and LDH production. Since the permeation results showed that cream was the most favourable vehicle, FX-cream was elaborated. This formulation effectively ameliorated TPA-induced hyperplasia, by reducing skin edema, epidermal thickness, MPO activity and COX-2 expression. Moreover, FX-cream reduced UVB-induced erythema through down-regulation of COX-2 and iNOS as well as up-regulation of HO-1 protein via Nrf-2 pathway. In conclusion, FX, administered in a topical formulation, could be a novel natural adjuvant for preventing exacerbations associated with skin inflammatory pathologies as well as protecting skin against UV radiation.
Scabiosa Genus: A Rich Source of Bioactive Metabolites
Authors: Pinto Diana C. G. A. , Rahmouni Naima , Beghidja Noureddine , Silva Artur M. S.
Published: October 2018

The genus Scabiosa (family Caprifoliaceae) is considered large (618 scientific plant names of species) although only 62 have accepted Latin binominal names. The majority of the Scabiosa species are widely distributed in the Mediterranean region and some Scabiosa species are used in traditional medicine systems. For instance, Scabiosa columbaria L. is used traditionally against diphtheria while S. comosa Fisch. Ex Roem. and Schult. is used in Mongolian and Tibetan traditional medical settings to treat liver diseases. The richness of Scabiosa species in secondary metabolites such as iridoids, flavonoids and pentacyclic triterpenoids may contribute to its use in folk medicine. Details on the most recent and relevant pharmacological in vivo studies on the bioactive secondary metabolites isolated from Scabiosa species will be summarized and thoroughly discussed.
Sleep and Delirium in Pediatric Critical Illness: What Is the Relationship?
Authors: Calandriello Amy , Tylka Joanna C. , Patwari Pallavi P.
Published: October 2018

With growing recognition of pediatric delirium in pediatric critical illness there has also been increased investigation into improving recognition and determining potential risk factors. Disturbed sleep has been assumed to be one of the key risk factors leading to delirium and is commonplace in the pediatric critical care setting as the nature of intensive care requires frequent and invasive monitoring and interventions. However, this relationship between sleep and delirium in pediatric critical illness has not been definitively established and may, instead, reflect significant overlap in risk factors and consequences of underlying neurologic dysfunction. We aim to review the existing tools for evaluation of sleep and delirium in the pediatric critical care setting and review findings from recent investigations with application of these measures in the pediatric intensive care unit.
Investigations of Alkaline and Enzymatic Membrane Cleaning of Ultrafiltration Membranes Fouled by Thermomechanical Pulping Process Water
Authors: Rudolph Gregor , Schagerlöf Herje , Morkeberg Krogh Kristian B. , Jönsson Ann-Sofi , Lipnizki Frank
Published: October 2018

The pulp and paper industry is one of the most important industrial sectors worldwide, and has considerable potential for the sustainable fractionation of lignocellulosic biomass to provide valuable compounds. Ultrafiltration (UF) is a suitable separation technique for the profitable production of hemicelluloses from process water from thermomechanical pulping (ThMP), but is limited by membrane fouling. Improvements in cleaning protocols and new alternative cleaning agents are required to ensure a long membrane lifetime, and thus a sustainable process. This study, therefore, focuses on the cleaning of polymeric UF membranes after the filtration of ThMP process water, comparing alkaline with enzymatic cleaning agents. The aim was to develop a cleaning procedure that is efficient under mild conditions, resulting in a lower environmental impact. It was not possible to restore the initial permeability of the membrane when cleaning the membrane with enzymes alone, but the permeability was restored when using a two-step cleaning process with enzymes in the first step and an alkaline cleaning agent in the second step. Scanning electron microscopy gave a deeper inside into the cleaning efficiency. Attenuated total reflectance Fourier-transform infrared spectroscopy analysis confirmed that not only polysaccharides, but also extractives are adsorbed onto the membrane surface.
A Method for Predicting the Effects of Specimen Geometry and Loading Condition on Fatigue Strength
Authors: Sun Chengqi , Song Qingyuan
Published: October 2018

Specimen geometry and loading condition usually have a great influence on the fatigue strength of metallic materials, which is an important issue in evaluating the reliability of component parts. In this paper, a rotating bending fatigue test is performed at first on an hourglass specimen and a notch specimen of a high strength titanium alloy. Experimental results indicate that, in terms of local stress, the notch specimen endures higher fatigue strength in comparison with the hourglass specimen due to its relatively smaller control volume. Then, a probabilistic control volume method is proposed for correlating the effects of specimen geometry and loading condition on the fatigue strength based on Weibull distribution and the concept of control volume. A simple formula is obtained for the fatigue strength in relation to control volumes, in which the parameter is the shape parameter of Weibull distribution of fatigue strength. The predicted results are in good agreement with the present experimental data for high strength titanium alloy and the data for the high strength steel and the full scale EA4T axle in the literature.
Comparative Rice Bran Metabolomics across Diverse Cultivars and Functional Rice Gene–Bran Metabolite Relationships
Authors: Zarei Iman , Luna Emily , Leach Jan E. , McClung Anna , Vilchez Samuel , Koita Ousmane , Ryan Elizabeth P.
Published: October 2018

Rice (Oryza sativa L.) processing yields ~60 million metric tons of bran annually. Rice genes producing bran metabolites of nutritional and human health importance were assessed across 17 diverse cultivars from seven countries using non-targeted metabolomics, and resulted in 378–430 metabolites. Gambiaka cultivar had the highest number and Njavara had the lowest number of metabolites. The 71 rice bran compounds of significant variation by cultivar included 21 amino acids, seven carbohydrates, two metabolites from cofactors and vitamins, 33 lipids, six nucleotides, and two secondary metabolites. Tryptophan, α-ketoglutarate, γ-tocopherol/β-tocopherol, and γ-tocotrienol are examples of bran metabolites with extensive cultivar variation and genetic information. Thirty-four rice bran components that varied between cultivars linked to 535 putative biosynthetic genes using to the OryzaCyc 4.0, Plant Metabolic Network database. Rice genes responsible for bran composition with animal and human health importance is available for rice breeding programs to utilize in crop improvement.
Generation of Color Images by Utilizing a Single Composite Diffractive Optical Element
Authors: Wang Jiazhou , Liu Liwei , Cao Axiu , Pang Hui , Xu Chuntao , Mu Quanquan , Chen Jian , Shi Lifang , Deng Qiling
Published: October 2018

This paper presents an approach that is capable of producing a color image using a single composite diffractive optical element (CDOE). In this approach, the imaging function of a DOE and the spectral deflection characteristics of a grating were combined together to obtain a color image at a certain position. The DOE was designed specially to image the red, green, and blue lights at the same distance along an optical axis, and the grating was designed to overlay the images to an off-axis position. We report the details of the design process of the DOE and the grating, and the relationship between the various parameters of the CDOE. Following the design and numerical simulations, a CDOE was fabricated, and imaging experiments were carried out. Both the numerical simulations and the experimental verifications demonstrated a successful operation of this new approach. As a platform based on coaxial illumination and off-axis imaging, this system is featured with simple structures and no cross-talk of the light fields, which has huge potentials in applications such as holographic imaging.
Integrity Assessment of a Hybrid DBS Probe that Enables Neurotransmitter Detection Simultaneously to Electrical Stimulation and Recording
Authors: Ashouri Vajari Danesh , Vomero Maria , Erhardt Johannes , Sadr Ali , Ordonez Juan , Coenen Volker , Stieglitz Thomas
Published: October 2018

Deep brain stimulation (DBS) is a successful medical therapy for many treatment resistant neuropsychiatric disorders such as movement disorders; e.g., Parkinson’s disease, Tremor, and dystonia. Moreover, DBS is becoming more and more appealing for a rapidly growing number of patients with other neuropsychiatric diseases such as depression and obsessive compulsive disorder. In spite of the promising outcomes, the current clinical hardware used in DBS does not match the technological standards of other medical applications and as a result could possibly lead to side effects such as high energy consumption and others. By implementing more advanced DBS devices, in fact, many of these limitations could be overcome. For example, a higher channels count and smaller electrode sites could allow more focal and tailored stimulation. In addition, new materials, like carbon for example, could be incorporated into the probes to enable adaptive stimulation protocols by biosensing neurotransmitters in the brain. Updating the current clinical DBS technology adequately requires combining the most recent technological advances in the field of neural engineering. Here, a novel hybrid multimodal DBS probe with glassy carbon microelectrodes on a polyimide thin-film device assembled on a silicon rubber tubing is introduced. The glassy carbon interface enables neurotransmitter detection using fast scan cyclic voltammetry and electrophysiological recordings while simultaneously performing electrical stimulation. Additionally, the presented DBS technology shows no imaging artefacts in magnetic resonance imaging. Thus, we present a promising new tool that might lead to a better fundamental understanding of the underlying mechanism of DBS while simultaneously paving our way towards better treatments.
Petrography and Organic Geochemistry Characterizations of Lower Paleozoic Organic-Rich Shale in the Northwestern Upper Yangtze Plate: Niutitang Formation and Longmaxi Formation, Dabashan Foreland Belt
Authors: Li Delu , Li Rongxi , Zhao Di , Xu Feng
Published: October 2018

Measurements of total organic carbon, Rock-Eval pyrolysis, X-ray diffraction, scanning electron microscope, maceral examination, gas chromatography, and gas chromatography-mass spectrometry were conducted on the organic-rich shale of Lower Paleozoic Niutitang Formation and Longmaxi Formation in Dabashan foreland belt to discuss the organic matter characteristic, organic matter origin, redox condition, and salinity. The results indicate that the Niutiang Formation and Longmaxi Formation organic-rich shale are good and very good source rocks with Type I kerogen. Both of the shales have reached mature stage for generating gas. Biomarker analyses indicate that the organic matter origin of Niutitang Formation and Longmaxi Formation organic-rich shale are all derived from the lower bacteria and algae, and the organic matter are all suffered different biodegradation degrees. During Niutitang Formation and Longmaxi Formation period, the redox conditions are both anoxic with no stratification and the sedimentary water is normal marine water.
Mullite Stabilized Palmitic Acid as Phase Change Materials for Thermal Energy Storage
Authors: Gu Xiaobin , Liu Peng , Bian Liang , Peng Lihua , Liu Yungui , He Huichao
Published: October 2018

In this paper, mullite was adopted in order to absorb Palmitic Acid (PA) via a direct impregnation method. The prepared PA/mullite form-stable phase change materials (FSPCM) were systematically characterized by the Leakage Test (LT), Scanning Electron Microscope (SEM), Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction (XRD), Differential Scanning Calorimeter (DSC), Thermogravimetry (TG) and Cooling Curve Method (CCM). The results indicated that, among these composites with different mass fractions of PA, the sample with the 32 wt % Palmitic Acid has the best properties without any leakage. The enthalpy of 32%PA/68%mullite FSPCM is 50.8 J/g for melting process, and 58.3 J/g for solidifying process. The phase change point of 32%PA/68%mullite FSPCM is 64.1 °C for melting and 58.7 °C for solidifying. The heat storage efficiency of the PA/mullite FSPCM was enhanced considerably by adding mullite. The leakage and thermal properties of PA/mullite FSPCM were discussed and the performance of the FSPCM has been apparently improved.
Pharmacokinetic, Antiproliferative and Apoptotic Effects of Phenolic Acids in Human Colon Adenocarcinoma Cells Using In Vitro and In Silico Approaches
Authors: Rosa Lana , Jordão Nathállia , Da Costa Pereira Soares Nathália , DeMesquita Joelma , Monteiro Mariana , Teodoro Anderson
Published: October 2018

Colon cancer is the second most common cause of cancer deaths in the USA and Europe. Despite aggressive therapies, many tumors are resistant to current treatment protocols and epidemiological data suggest that diet is a major factor in the etiology of colon cancer. This study aimed to evaluate the antioxidant activity and the influence of 3,4-dihydroxyphenylacetic (3,4-DHPAA), p-coumaric (p-CoA), vanillic (VA) and ferulic (FA) acids on cell viability, cell cycle progression, and rate of apoptosis in human colon adenocarcinoma cells (HT-29). The results showed that all compounds tested reduce cell viability in human colon cancer cells. 3,4-DHPAA promoted the highest effect antiproliferative with an increase in the percentage of cells in G0/G1 phase, accompanied by a reduction of cells in G2/M phase. Cell cycle analysis of VA and FA showed a decrease in the proportion of cells in G0/G1 phase (10.0 µM and 100.0 µM). p-CoA and FA acids increased the percentage of apoptotic cells and non-apoptotic cells. 3,4-DHPAA seems to be the substance with the greatest potential for in vivo studies, opening thus a series of perspectives on the use of these compounds in the prevention and treatment of colon cancer.
Identification of Alkaloids from Corydalis yanhusuo W. T. Wang as Dopamine D1 Receptor Antagonists by Using CRE-Luciferase Reporter Gene Assay
Authors: Wu Lehao , Zhang Weiyue , Qiu Xin , Wang Chaoran , Liu Yanfang , Wang Zhiwei , Yu Yang , Ye Richard D. , Zhang Yan
Published: October 2018

Corydalis yanhusuo W. T. Wang (C. yanhusuo) has been traditionally used for drug addiction and pain relief in China. In our previous study, we showed that the extract of C. yanhusuo blocks dopamine receptors, demonstrating that its pharmacological activities are mostly due to the antagonistic effects of some of its components at dopamine receptors. As part of our ongoing project on C. yanhusuo, the aim of the present study is to establish a high-throughput and low-cost screening assay system and test the abilities of the isolated alkaloids from C. yanhusuo to inhibit dopamine-induced dopamine D1 receptor activity. By using our established cyclic adenosine monophosphate (cAMP)-response element (CRE)-luciferase reporter gene assay system, we identified eight alkaloids from C. yanhusuo with D1 receptor antagonistic activities. We next validated the activities of these compounds using fluorometric imaging plate reader (FLIPR) assay by measuring the intracellular Ca2+ change. Six out of eight compounds, including tetrahydropalmatine, corydaline, 13-methyldehydrocorydalmine, dehydrocorybubine, dehydrocorydaline, and columbamine, can be confirmed for their inhibitory activities. The dopamine-receptor-antagonistic effects of four compounds, including 13-methyldehydrocorydalmine, dehydrocorydaline, columbamine, and corydaline, are reported for the first time. The present study provides an important pharmacological basis to support the traditional use of C. yanhusuo in China.
Melamine Sponge Functionalized with Urea-Formaldehyde Co-Oligomers as a Sorbent for the Solid-Phase Extraction of Hydrophobic Analytes
Authors: García-Valverde María Teresa , Chatzimitakos Theodoros , Lucena Rafael , Cárdenas Soledad , Stalikas Constantine D.
Published: October 2018

A new procedure for the functionalization of melamine sponge (MeS) with urea-formaldehyde (UF) co-oligomers is put forward. The procedure differs from the typical synthesis of the UF co-polymer, as it employs a base-catalyzed condensation step at certain concentrations of urea and formaldehyde. The produced melamine-urea-formaldehyde (MUF) sponge cubes are hydrophobic, despite the presence of hydrophilic groups in the oligomers. The MUF sponge developed herein is used as a sorbent for the solid-phase extraction of 10 analytes, from 6 different classes (i.e., non-steroidal anti-inflammatory drugs, benzophenones, parabens, phenols, pesticides and musks) and an analytical method is developed for their liquid chromatographic separation and detection. Low limits of quantification (0.03 and 1.0 μg L−1), wide linear ranges and excellent recoveries (92–100%) are some of the benefits of the proposed procedure. The study of the synthesis conditions of MUF cubes reveals that by altering them the hydrophilic/lipophilic balance of the MUF cubes can be tuned, hinting towards a strong potential for many other applications.
Improved Application of Carbon Nanotube Atomic Force Microscopy Probes Using PeakForce Tapping Mode
Authors: Slattery Ashley D. , Shearer Cameron J. , Shapter Joseph G. , Blanch Adam J. , Quinton Jamie S. , Gibson Christopher T.
Published: October 2018

In this work PeakForce tapping (PFT) imaging was demonstrated with carbon nanotube atomic force microscopy (CNT-AFM) probes; this imaging mode shows great promise for providing simple, stable imaging with CNT-AFM probes, which can be difficult to apply. The PFT mode is used with CNT-AFM probes to demonstrate high resolution imaging on samples with features in the nanometre range, including a Nioprobe calibration sample and gold nanoparticles on silicon, in order to demonstrate the modes imaging effectiveness, and to also aid in determining the diameter of very thin CNT-AFM probes. In addition to stable operation, the PFT mode is shown to eliminate “ringing” artefacts that often affect CNT-AFM probes in tapping mode near steep vertical step edges. This will allow for the characterization of high aspect ratio structures using CNT-AFM probes, an exercise which has previously been challenging with the standard tapping mode.
Systemic Administration of Polyelectrolyte Microcapsules: Where Do They Accumulate and When? In Vivo and Ex Vivo Study
Authors: Navolokin Nikita A. , German Sergei V. , Bucharskaya Alla B. , Godage Olga S. , Zuev Viktor V. , Maslyakova Galina N. , Pyataev Nikolaiy A. , Zamyshliaev Pavel S. , Zharkov Mikhail N. , Terentyuk Georgy S. , Gorin Dmitry A. , Sukhorukov Gleb B.
Published: October 2018

Multilayer capsules of 4 microns in size made of biodegradable polymers and iron oxide magnetite nanoparticles have been injected intravenously into rats. The time-dependent microcapsule distribution in organs was investigated in vivo by magnetic resonance imaging (MRI) and ex vivo by histological examination (HE), atomic absorption spectroscopy (AAS) and electron spin resonance (ESR), as these methods provide information at different stages of microcapsule degradation. The following organs were collected: Kidney, liver, lung, and spleen through 15 min, 1 h, 4 h, 24 h, 14 days, and 30 days after intravenous injections (IVIs) of microcapsules in a saline buffer at a dosage of 2.5 × 109 capsule per kg. The IVI of microcapsules resulted in reversible morphological changes in most of the examined inner organs (kidney, heart, liver, and spleen). The capsules lost their integrity due to degradation over 24 h, and some traces of iron oxide nanoparticles were seen at 7 days in spleen and liver structure. The morphological structure of the tissues was completely restored one month after IVI of microcapsules. Comprehensive analysis of the biodistribution and degradation of entire capsules and magnetite nanoparticles as their components gave us grounds to recommend these composite microcapsules as useful and safe tools for drug delivery applications.
Role of Functional Beverages on Sport Performance and Recovery
Authors: Orrù Stefania , Imperlini Esther , Nigro Ersilia , Alfieri Andreina , Cevenini Armando , Polito Rita , Daniele Aurora , Buono Pasqualina , Mancini Annamaria
Published: October 2018

Functional beverages represent a palatable and efficient way to hydrate and reintegrate electrolytes, carbohydrates, and other nutrients employed and/or lost during physical training and/or competitions. Bodily hydration during sporting activity is one of the best indicators of health in athletes and can be a limiting factor for sport performance. Indeed, dehydration strongly decreases athletic performance until it is a risk to health. As for other nutrients, each of them is reported to support athletes’ needs both during the physical activity and/or in the post-workout. In this study, we review the current knowledge of macronutrient-enriched functional beverages in sport taking into account the athletes’ health, sports performance, and recovery.
Dietary Protein Sources and Muscle Mass over the Life Course: The Lifelines Cohort Study
Authors: Alexandrov Nikita V. , Eelderink Coby , Singh-Povel Cécile M. , Navis Gerjan J. , Bakker Stephan J. L. , Corpeleijn Eva
Published: October 2018

The influence of dietary protein intake on muscle mass in adults remains unclear. Our objective was to investigate the association between protein intake and muscle mass in 31,278 men and 45,355 women from the Lifelines Cohort. Protein intake was estimated by food frequency questionnaire and muscle mass was estimated from 24 h urinary creatinine excretion. The age range was 18–91 years and mean total protein intake was 1.0 ± 0.3 g/kg/day. Across increasing quartiles of total protein intake, animal protein intake, and fish/meat/egg protein intake, creatinine excretion significantly increased in both men (+4% for total and +6% for fish/meat/egg protein intake, p < 0.001) and women (+3% for total and +6% for fish/meat/egg protein intake, p < 0.001). The associations were not systematically stronger or weaker with increasing age, but associations were strongest for young men (26–45 years) and older women (>75 years). The association between total protein intake and muscle mass was dependent on physical activity in women (p interaction < 0.001). This study suggests that total protein intake, animal protein intake, and in particular fish/meat/egg protein intake may be important for building and preserving muscle mass. Dietary protein sources should be further studied for their potential to build and preserve muscle mass.
Filling the Gaps: Current Research Directions for a Rational Use of Probiotics in Preterm Infants
Authors: Aceti Arianna , Beghetti Isadora , Maggio Luca , Martini Silvia , Faldella Giacomo , Corvaglia Luigi
Published: October 2018

The use of probiotics among very low-birth-weight infants is constantly increasing, as probiotics are believed to reduce the incidence of severe diseases such as necrotizing enterocolitis and late-onset sepsis and to improve feeding tolerance. However, despite the enthusiasm towards these products in neonatal medicine, theoretical knowledge and clinical applications still need to be improved. The purpose of this review is to give an overview of the most important gaps in the current literature about potential uses of probiotics in preterm infants, highlighting promising directions for future research. Specifically, further well-designed studies should aim at clarifying the impact of the type of feeding (mother’s milk, donor milk, and formula) on the relationship between probiotic supplementation and clinical outcome. Moreover, future research is needed to provide solid evidence about the potential greater efficacy of multi-strain probiotics compared to single-strain products. Safety issues should also be addressed properly, by exploring the potential of paraprobiotics and risks connected to antibiotic resistance in preterm infants. Last, in light of increasing commercial and public interests, the long-term effect of routine consumption of probiotics in such a vulnerable population should be also evaluated.
The Effectiveness of a Smartphone Application on Modifying the Intakes of Macro and Micronutrients in Primary Care: A Randomized Controlled Trial. The EVIDENT II Study
Authors: Recio-Rodriguez Jose I. , Agudo Conde Cristina , Calvo-Aponte Maria J. , Gonzalez-Viejo Natividad , Fernandez-Alonso Carmen , Mendizabal-Gallastegui Nere , Rodriguez-Martin Beatriz , Maderuelo-Fernandez Jose A. , Rodriguez-Sanchez Emiliano , Gomez-Marcos Manuel A. , Garcia-Ortiz Luis ,   On Behalf of the EVIDENT Investigators
Published: October 2018

Background: This study evaluates the effectiveness of adding a diet smartphone application to standard counseling to modify dietary composition over the long term (12 months). Methods: A randomized, controlled, multicenter clinical trial was conducted involving the participation of 833 subjects from primary care clinics (415 to the intervention (counseling + application) group (IG) and 418 to the control (counseling) group (CG)). Both groups were counseled about a healthy diet and physical activity. For the 3-month intervention period, the IG was also trained to use a diet smartphone application that involved dietary self-monitoring and tailored feedback. Nutritional composition was estimated using a self-reported food frequency questionnaire. Results: An analysis of repeated measures revealed an interaction between the group and the percentages of carbohydrates (p = 0.031), fats (p = 0.015) and saturated fats (p = 0.035) consumed. Both groups decreased their energy intake (Kcal) at 12 months (IG: −114 (95% CI: −191 to −36); CG: −108 (95% CI: −184 to −31)). The IG reported a higher percentage intake of carbohydrates (1.1%; 95% CI: 0.1 to 2.0), and lower percentage intakes of fats (−1.0%; 95% CI: −1.9 to −0.1) and saturated fats (−0.4%; 95%CI: −0.8 to −0.1) when compared to the CG. Conclusions: Better results were achieved in terms of modifying usual diet composition from counseling and the diet smartphone application compared to counseling alone. This was evaluated by a self-reported questionnaire, which indicated an increased percentage intake of carbohydrates, and decreased percentage intakes of fats and saturated fats.
Some Key Factors Influencing the Flame Retardancy of EDA-DOPO Containing Flexible Polyurethane Foams
Authors: Przystas Agnieszka , Jovic Milijana , Salmeia Khalifah A. , Rentsch Daniel , Ferry Laurent , Mispreuve Henri , Perler Heribert , Gaan Sabyasachi
Published: October 2018

The role of various additives (emulsifier, anti-dripping agent) and formulation procedures (pre-dispersion of solid additives in polyol via milling) which influence the flame retardancy of 6,6′-[ethan-1,2-diylbis(azandiyl)]bis(6H-dibenzo[c,e][1,2]oxaphosphin-6-oxid) (EDA-DOPO) containing flexible polyurethane foams has been investigated in this work. For comparison, the flame retardancy of two additional structurally-analogous bridged 9,10-dihydro-9-oxa-10-phosphaphenanthrene-10-oxide (DOPO)-based compounds, i.e., ethanolamine-DOPO (ETA-DOPO) and ethylene glycol-DOPO (EG-DOPO) were also evaluated together with EDA-DOPO in flexible PU foams of various formulations. The flame retardancy of these three bridged-DOPO compounds depends on the type of PU formulation. For certain PU formulations containing EDA-DOPO, lower fire performance was observed. Addition of emulsifier and polytetrafluoroethylene (PTFE) to these PU formulations influenced positively the flame retardancy of EDA-DOPO/PU foams. In addition, dispersion of EDA-DOPO and PTFE via milling in polyol improved the flame retardancy of the PU foams. Mechanistic studies performed using a microscale combustion calorimeter (MCC) and its coupling to FTIR showed no difference in the combustion efficiency of the bridged-DOPO compounds in PU foams. From MCC experiments it can be concluded that these bridged-DOPO compounds and their decomposition products may work primarily in the gas phase as flame inhibitors. The physiochemical behavior of additives in PU formulation responsible for the improvement in the flame retardancy of PU foams was further investigated by studying the dripping behavior of the PU foams in the UL 94 HB test. A high-speed camera was used to study the dripping behavior in the UL 94 HB test and results indicate a considerable reduction of the total number of melt drips and flaming drips for the flame retardant formulations. This reduction in melt drips and flaming drips during the UL 94 HB tests help PU foams achieve higher fire classification.
Temperature Dependence of the Pore Structure in Polyvinylidene Fluoride (PVDF)/Graphene Composite Membrane Probed by Electrochemical Impedance Spectroscopy
Authors: Luo Qizhao , Huang Qing , Chen Zhe , Yao Lei , Fu Qiuming , Fu Ping , Lin Zhidong
Published: October 2018

In this paper, graphene was introduced in the PVDF to improve the thermal stability of the pore structure, which is the key feature for the membrane applied for the thermo-osmotic energy conversion (TOEC) process. The PVDF/graphene composite membranes were characterized by a scanning electron microscopy (SEM), a water contact angle measurement, and electrochemical impedance spectroscopy (EIS). It was found that the composite membranes exhibited improved surface hydrophobicity. Moreover, the pores in pure PVDF membrane would expand during the heat process while the existence of graphene in PVDF clearly suppressed the expansion, which implied better thermal stability of the pores in the composite membrane. According to the pore deformation time, the heat conductivities of the membranes were calculated and compared with each other. It confirmed that the composite membrane with higher graphene content exhibited enhanced heat conductivity. EIS can be used to monitor the temperature dependence of the pore structure in aqueous environments.
Living and Dealing with Food in an Affluent Society—A Case for the Study of Lived (Non)Religion
Authors: Salonen Anna Sofia
Published: October 2018

Significant changes have been taking place in the field of the sociology of religion in the last few decades, which challenge researchers to rethink this scholarly field. This article suggests that a great deal could be learned about the current dilemmas within this field through research that explores the moral underpinnings of everyday food consumption within contemporary society that is characterized by abundance. More specifically, the article proposes that everyday food consumption and everyday ethics provide unique opportunities to transcend and surpass crucial distinctions within social sciences in a way that can feed the sociological imagination in relation to research on lived (non)religion. Drawing on examples from research on food consumption in the nonreligious context and at the individual, discursive and institutional levels, this study shows how the everyday ethics of food consumption can serve as a point of departure for sociological research, which could help researchers to understand the currents of lived religion and nonreligion in a way that evades the idea of religion as a certain set of practices or beliefs, or as a specific religious affiliation. This research would enable the study of issues such as practices, beliefs, meanings and belonging, as well as distancing, withdrawal, and indifference.
Hate in a Tweet: Exploring Internet-Based Islamophobic Discourses
Authors: Evolvi Giulia
Published: October 2018

Islamophobia is the unfounded hostility against Muslims. While anti-Muslim feelings have been explored from many perspectives and in different settings, Internet-based Islamophobia remains under-researched. What are the characteristics of online Islamophobia? What are the differences (if any) between online and offline anti-Muslim narratives? This article seeks to answer these questions through a qualitative analysis of tweets written in the aftermath of the 2016 British referendum on European Union membership (also known as “Brexit”), which was followed by a surge of Islamophobic episodes. The analysis of the tweets suggests that online Islamophobia largely enhances offline anti-Islam discourses, involving narratives that frame Muslims as violent, backward, and unable to adapt to Western values. Islamophobic tweets also have some peculiar characteristics: they foster global networks, contain messages written by so-called “trolls” and “bots,” and contribute to the spreading of “fake news.” The article suggests that, in order to counteract online Islamophobia, it is important to take into account the networked connections among social media, news media platforms, and offline spaces.