bluuebunny/crossref_metadata_2025 huggingface dataset:
Minimally invasive percutaneous plate osteosynthesis versus intramedullary nail fixation for distal tibial fractures: a systematic review and meta-analysis
Authors: Wang Bo , Zhao Yang , Wang Qian , Hu Bin , Sun Liang , Ren Cheng , Li Zhong , Zhang Kun , Hao Dingjun , Ma Teng , Lu Yao
Published: December 2019

Background

The treatment for distal tibial fractures remains controversial to date. Minimally invasive percutaneous plate osteosynthesis (MIPPO) and intramedullary nailing (IMN) are well-accepted and effective methods for distal tibial fractures, but these methods were associated with complications. This study aimed to assess and compare the clinical and functional outcomes in patients with distal tibial fractures treated with MIPPO or IMN.

Methods

We systematically reviewed randomized controlled trials (RCTs) that compared MIPPO with IMN in patients with distal tibial fractures from inception till 15 August 2019. Also, quantitative summaries of time to reunion, rate of complications, and functional outcomes were evaluated.

Results

The pooled results suggested that patients in the MIPPO group had a longer time to reunion with a mean difference of 1.21 weeks [P= 0.02; 95% confidence interval (CI) 0.16–2.26)] than those in the IMN group. The overall union complications and deep infection between IMN and MIPPO were similar (P> 0.05). IMN had a significantly low risk of wound complications [risk ratio (RR) = 0.51,P= 0.00, 95% CI 0.34–0.77)]. The pooled functional outcomes of the two groups remained controversial by different evaluating scores.

Conclusions

Compared to MIPPO, IMN had a significantly low risk of wound complications and associated with limited time for reunion. Although the pooled functional outcomes of the two groups were controversial due to different evaluating scores, IMN was the preferred surgical technique than MIPPO for treating distal tibial fractures.
Open reduction and compression with double Kirschner wires for the treatment of old bony mallet finger
Authors: Tang Junjun , Wu Kejian , Wang Jinchang , Zhang Jian
Published: December 2019

Background

The management of old bony mallet fingers is complicated. The aim of the study is to present a new method of open reduction and compression with double Kirschner wires (K-wires) in treating old bony mallet fingers.

Methods

This was a retrospective analysis of patients with old closed bony mallet fingers treated between June 2013 and December 2016. Complications were observed. The range of motion (ROM) of the DIP joints was measured using a protractor. At the last follow-up, anteroposterior and lateral X-ray of the affected finger was performed, the flatness of the articular surface was scored, and the results were graded using Crawford’s criteria.

Results

Seventeen patients were followed up for 8 (6–19) months. The width of the avulsion fracture block accounted for 25–62% of the articular surface of the distal phalanx. Twelve (70.6%) patients had anterior dislocation of the interphalangeal joint. All patients reported healing at the fracture sites. Healing time was 7.6 ± 2.1(5–13) weeks. All patients had incision healing of I/Class A. Lateral X-ray showed 13 and four patients had excellent and good articular surface flatness, respectively. At the last follow-up, no traumatic arthritis was present. Only one patient developed mild pain after surgery (VAS score of 3). Postoperative ROM was 76.5 ± 10.6° (P = 0.0625 vs. healthy side). At the last follow-up, the angle of loss of dorsiflexion was 0–10° (P < 0.0001 vs. baseline). The flexion angle was 50–90° (P = 0.0625 vs. healthy side).

Conclusions

Open reduction and compression with double K-wires is feasible in treating old bony mallet finger.
Associations between serum calcium, 25(OH)D level and bone mineral density in older adults
Authors: Liu Minbo , Yao Xiaocong , Zhu Zhongxin
Published: December 2019

Background

Calcium and vitamin D play important roles in bone health as essential nutrients. We explored whether serum calcium, 25(OH)D were associated with bone mineral density (BMD) in older adults.

Methods

This cross-sectional study was conducted on a sample of 4595 participants (2281 men and 2314 women) aged ≥ 50 years (from 50 to 85 years, 60.1 ± 8.7 years for men and 62.0 ± 9.7 years for women) from the National Health and Nutrition Examination Survey (NHANES) 2001–2006. The independent variables were serum calcium and 25(OH)D. The dependent variable was lumbar BMD. The other variables were considered potential effect modifiers. We performed weighted multivariate linear regression models and smooth curve fittings to evaluate the associations between them. Subgroup analyses were also performed.

Results

We observed a negative association between serum calcium and lumbar BMD in the fully adjusted model. In the subgroup analyses, this association was no longer significant among males and other race/ethnicity. On the other hand, there was a positive association between serum 25(OH)D and lumbar BMD in the fully adjusted model. In the subgroup analyses, this association did not differ in different age groups, between men and women. However, the association between serum 25(OH)D and lumbar BMD followed a U-shaped curve in Mexican Americans.

Conclusions

This cross-sectional study indicated that serum calcium negatively correlated with lumbar BMD, and serum 25(OH)D positively correlated with lumbar BMD in older adults. However, the association between serum calcium and lumbar BMD in males followed a U-shaped curve.
A case of prenatal diagnosis of 18p deletion syndrome following noninvasive prenatal testing
Authors: Zhao Ganye , Dai Peng , Gao Shanshan , Zhao Xuechao , Wang Conghui , Liu Lina , Kong Xiangdong
Published: December 2019

Background

Chromosome 18p deletion syndrome is a disease caused by the complete or partial deletion of the short arm of chromosome 18, there were few cases reported about the prenatal diagnosis of 18p deletion syndrome. Noninvasive prenatal testing (NIPT) is widely used in the screening of common fetal chromosome aneuploidy. However, the segmental deletions and duplications should also be concerned. Except that some cases had increased nuchal translucency or holoprosencephaly, most of the fetal phenotype of 18p deletion syndrome may not be evident during the pregnancy, 18p deletion syndrome was always accidentally discovered during the prenatal examination.

Case presentations

In our case, we found a pure partial monosomy 18p deletion during the confirmation of the result of NIPT by copy number variation sequencing (CNV-Seq). The result of NIPT suggested that there was a partial or complete deletion of X chromosome. The amniotic fluid karyotype was normal, but result of CNV-Seq indicated a 7.56 Mb deletion on the short arm of chromosome 18 but not in the couple, which means the deletion was de novo deletion. Finally, the parents chose to terminate the pregnancy.

Conclusions

To our knowledge, this is the first case of prenatal diagnosis of 18p deletion syndrome following NIPT.NIPT combined with ultrasound may be a relatively efficient method to screen chromosome microdeletions especially for the 18p deletion syndrome.
Chinese herbal medicine Tangshen Formula treatment for type 2 diabetic kidney disease in the early stage: study protocol for a randomized controlled trial
Authors: Jin De , Huang Wen-Jing , Meng Xiang , Yang Fan , Bao Qi , Zhang Mei-zhen , Yang Ya-nan , Ni Qing , Lian Feng-Mei , Tong Xiao-Lin
Published: December 2019

Background

Diabetic kidney disease (DKD) is the main cause of end-stage kidney disease and has become a heavy economic and social burden due to its high prevalence and morbidity. The most effective strategy is that patients with DKD should be diagnosed and treated early. Preliminary studies showed that the Chinese herbal Tangshen Formula (TSF) may delay the progression of DKD, reducing microalbuminuria and macroalbuminuria and improving renal function. We designed a randomized, double-blind, placebo-controlled trial to evaluate the efficacy of TSF in patients with DKD.

Methods/design

This trial is a 13-center, randomized, double-blind, placebo-controlled study. A total of 632 participants will be randomized in a 1:1 ratio to an experiment group (TSF plus losartan) and a control group (placebo plus losartan). The trial cycle will last 24 weeks. The primary outcome will be the change in the urine microalbumin–creatinine ratio from baseline to week 24. The secondary outcome will be the change in the rate of progression to the clinical proteinuria period after intervention, the rate of urine microalbumin negative conversion, the rate of normal urinary microalbumin, the doubling rate of the baseline creatinine value and the glomerular filtration rate between the two groups. Safety in medication will also be evaluated.

Discussion

We hypothesize that patients with type 2 diabetes in the early stage of DKD will benefit from TSF. If successful, this study will provide evidence-based recommendations for clinicians.

Trial registration

ClinicalTrials.gov, NCT03009864. Registered January 2017.
A disorder-specific group cognitive behavior therapy for social anxiety disorder in adolescents: study protocol for a randomized controlled study
Authors: Lassen Nanna Fensman , Hougaard Esben , Arendt Kristian Bech , Thastum Mikael
Published: December 2019

Background

Social anxiety disorder (SAD) is a common disorder in adolescence associated with extensive distress and long-term impairment. Generic cognitive behavior therapy (CBT) programs for anxiety disorders have shown poorer outcomes for adolescents with SAD than for other anxiety disorders.

Aim

The aim of the present study is to investigate the efficacy of a disorder-specific group cognitive behavior therapy (G-CBT) program for youth SAD, the Cool Kids Anxiety Program - Social Enhanced (CK-E), developed at Macquarie University, Sidney, Australia.

Methods

The study is a randomized controlled trial comparing CK-E to a generic G-CBT program for anxiety disorders. Approximately 96 adolescents aged 12 to 17 years are included with data points at pre- and post-treatment, and at 3 months and 1 year follow-ups.

Discussion

The current study will provide more information about the efficacy of diagnosis-specific G-CBT treatment for youth SAD.

Trial registration

ClinicalTrials.gov, NCT03986827. Registered on 14 June 2019.
Pressurized hot water extraction of hydrosable tannins from Phyllanthus tenellus Roxb.
Authors: Mohd Jusoh Noor Hidayah , Subki Atiqah , Yeap Swee Keong , Yap Ken Choy , Jaganath Indu Bala
Published: December 2019

Background

Safety, environmental and economic setbacks are driving industries to find greener approaches to extract bioactive compounds from natural resources. Pressurized hot water extraction (PHWE) is among the solvent free and efficient methods for extracting bioactive compounds.

Experimental

In this study, the suitability of PHWE for extracting bioactive compounds such as phenolics, hydrolysable tannins and flavonoids from Phyllanthus tenellus was investigated by UPLC-qTOF-MS.

Results

Solvent properties of water are significantly increased through imposing temperature at 121 °C and pressure at 15 p.s.i. Pressurized hot water extraction obtained 991-folds higher hydrolysable tannins than methanol extraction.

Conclusion

The extraction yields of hydrolysable tannins with PHWE was almost double of absolute methanol extraction.
Evaluation of an online, real-time, soft-photon ionisation time-of-flight mass spectrometer for mainstream tobacco smoke analysis
Authors: Hawke Jenni , Errington Graham , von Frowein Matthias Bente
Published: December 2019

Mainstream tobacco smoke is a complex and dynamic aerosol, consisting of particulate and vapour phases. Most approaches to determine mainstream smoke toxicant yields are based on offline techniques that limit the opportunity to observe in real time the processes leading to smoke formation. The recent development of online real-time analytical methods offers many advantages over traditional techniques. Here we report the LM2X-TOFMS (Borgwaldt GmbH, Germany), a commercial instrument that couples a linear smoking engine with a time-of-flight mass spectrometer for real-time per-puff measurement of the vapour phase of mainstream cigarette smoke. Total cigarette and puff-by-puff (μg/puff) yields were evaluated, in line with International Council of Harmonisation recommendations, for seven smoke toxicants: acetaldehyde, acetone, 1,3-butadiene, 2-butanone, benzene, isoprene and toluene. Measurements were unaffected by small system changes including replacing the sampling capillary or time of day (all P > 0.05), indicating that the LM2X-TOFMS is rugged. Control charts showed that the system has good stability and control. Analysis of certified gas mixtures of six concentrations of each analyte showed a highly linear response for all seven analytes (R2 = 0.9922–0.9999). In terms of repeatability, the lowest variation was observed for isoprene with a coefficient of variation (CV) of < 6% for each concentration. Acetaldehyde showed the highest CV, increasing from 8.0 to 26.6% with decreasing gas concentration. Accuracy was analysed in terms of relative error, which was ± 16% for six of the analytes; however, the relative error for acetaldehyde was (− 36.2%), probably due to its low ionisation efficiency under the instrument’s vacuum ultraviolet lamp. Three cigarette products (reference and commercial) with different ISO tar levels were analysed by the LM2X-TOFMS puff by puff under ISO regulatory smoking conditions. The relative standard deviation based on average yield per cigarette for each analyte in each product (summed puffs per product, n = 30) ranged from ≤ 9.3 to ≤ 16.2%. Measurements were consistent with published data per cigarette. In conclusion, the LM2X-TOFMS is suitable for determining the vapour-phase yields of seven analytes on a real-time, puff-by-puff basis, and can be utilised for both fast screening (qualitative) and quantitative measurements of mainstream cigarette smoke.
Enhanced angiogenic function in response to fibroblasts from psoriatic arthritis synovium compared to rheumatoid arthritis
Authors: Fromm S. , Cunningham C. C. , Dunne M. R. , Veale D. J. , Fearon U. , Wade S. M.
Published: December 2019

Introduction

Angiogenesis is an early event in the pathogenesis of both psoriatic arthritis (PsA) and rheumatoid arthritis (RA); however, there are striking differences in blood vessel morphology and activation between the two arthropathies. The aim of this study was to assess if the PsA and RA joint microenvironments differentially regulate endothelial cell function.

Methods

PsA and RA primary synovial fibroblasts (SFC) were isolated from synovial biopsies, grown to confluence, and supernatants harvested and termed ‘conditioned media’ (CM). Human umbilical vein endothelial cells (HUVEC) were cultured with PsA SFC or RA SFC-CM (20%). HUVEC tube formation, migration, and PBMC adhesion were assessed by matrigel tube formation, wound repair, and PBMC adhesion assays. HUVEC cell surface expression of ICAM, VCAM, and E-Selectin was assessed by flow cytometry. Transcriptome analysis of genes promoting angiogenesis was performed by real-time PCR. Finally, a MSD multiplex angiogenic assay was performed on PsA SFC and RA SFC supernatants.

Results

Macroscopic synovitis and vascularity were similar in PsA and RA patients; however, significant differences in vascular morphological pattern were recorded with tortuous, elongated vessels observed in PsA compared to straight regular branching vessels observed in RA. Transcriptome analysis showed strong upregulation of the pro-angiogenic signature in HUVEC primed with PsA SFC-CM compared to RA SFC-CM and basal control. In parallel, paired PsA SFC-CM significantly induced HUVEC tube formation compared to that of RA SFC-CM. Furthermore, PsA SFC-CM induced HUVEC migration was paralleled by a significant induction in VEGFA, PFKFB3, ICAM-1, and MMP3 mRNA expression. A significant increase in PBMC adhesion and cell surface expression of VCAM-1, ICAM-1, and E-Selectin expression was also demonstrated in PsA SFC-CM-primed HUVEC compared to RA SFC-CM. Finally, VEGF, TSLP, Flt-1, and Tie-2 expression was elevated in PsA SFC-CM compared to RA SFC-CM, with no significant difference in other pro-angiogenic mediators including MIP-3, bFGF, PIGF, and MCP-1.

Conclusion

PsA SFC and RA SFC secreted factors differentially regulate endothelial cell function, with soluble mediators in the PsA joint microenvironment inducing a more pro-angiogenic phenotype compared to the RA.
Dataset for file fragment classification of audio file formats
Authors: Khodadadi Atieh , Teimouri Mehdi
Published: December 2019

Objectives

File fragment classification of audio file formats is a topic of interest in network forensics. There are a few publicly available datasets of files with audio formats. Therewith, there is no public dataset for file fragments of audio file formats. So, a big research challenge in file fragment classification of audio file formats is to compare the performance of the developed methods over the same datasets.

Data description

In this study, we present a dataset that contains file fragments of 20 audio file formats: AMR, AMR-WB, AAC, AIFF, CVSD, FLAC, GSM-FR, iLBC, Microsoft ADPCM, MP3, PCM, WMA, A-Law, µ-Law, G.726, G.729, Microsoft GSM, OGG Vorbis, OPUS, and SPEEX. Corresponding to each format, the dataset contains the file fragments of audio files with different compression settings. For each pair of file format and compression setting, 210 file fragments are provided. Totally, the dataset contains 20,160 file fragments.
Early onset severe preeclampsia and eclampsia in a low-resource setting, Mpilo Central Hospital, Bulawayo, Zimbabwe
Authors: Ngwenya Solwayo , Jones Brian , Mwembe Desmond , Mapfumo Cladnos , Familusi Akinbowale , Nare Hausitoe , Heazell Alexander Edward Patrick
Published: December 2019

Objectives

Early-onset severe preeclampsia is associated with significant maternal and perinatal morbidity and mortality especially in low-resource settings, where women have limited access to antenatal care. This dataset was generated from a retrospective cross-sectional study carried out at Mpilo Central Hospital, covering the period February 1, 2016 to July 30, 2018. The aim of the study was to determine the incidence of early-onset severe preeclampsia and eclampsia, and associated risk factors in a low-resource setting. The reason for examining the incidence of preeclampsia specifically in a low-resource setting; was to document it as women in these settings appear to suffer from poor outcomes.

Data description

The dataset contains data of 238 pregnant women who had a diagnosis of early onset severe preeclampsia/eclampsia. There were 243 babies from singleton and twin gestations. There were five sets of twins. There were 21,505 live births during the study period giving an incidence of 1.1%. The dataset contains data on maternal socio-demographic, signs and symptoms, therapeutic interventions and mode of delivery, adverse outcomes characteristics, and fetal characteristics. This large dataset can be used to calculate the incidence and risk factors for adverse maternal and fetal outcomes or develop predictive models in severe preeclampsia/eclampsia.
Pay attention to the basal ganglia: a volumetric study in early dementia with Lewy bodies
Authors: Botzung Anne , Philippi Nathalie , Noblet Vincent , Loureiro de Sousa Paulo , Blanc Frédéric
Published: December 2019

Background

Cortical and subcortical cognitive impairments are usually found in dementia with Lewy bodies (DLB). Roughly, they comprise visuo-constructive/executive function and attention/processing speed impairments, whereas memory would remain relatively spared. In this study, we focused on the neuro-anatomical substrates of attention and processing speed, which is still poorly understood. For the purpose of the study, we examined the correlations between behavioral scores measuring the speed of processing and the degree of cerebral atrophy in patients with prodromal to moderate DLB.

Methods

Ninety-three prodromal to moderate DLB patients (mean MMSE = 25.5) were selected to participate in the study as well as 28 healthy elderly subjects (mean MMSE = 28.9), matched in terms of age and educational level. The Trail Making Test A (TMTA) and the Digit Symbol Substitution Test (DSST) were used to assess attention and processing speed. Behavioral performances were compared between patients and healthy control subjects. Three-dimensional MRI images were acquired for all participants, and correlational analyses were performed in the patient group using voxel-based morphometry (VBM).

Results

The behavioral results on both the TMTA (p = .026) and the DSST (p < .001) showed significantly impaired performances in patients in comparison with control subjects. In addition, correlational analyses using VBM revealed for the TMTA negative correlations in the caudate nucleus (left cluster peak significant at .05 FWE corrected), the putamen, the left thalamus, and the subthalamic nuclei (p < .05 FDR corrected). Some positive correlations associated with the DSST were found in the right inferior frontal gyrus, the left thalamus, and the left cerebellum (p < .001 uncorrected).

Conclusions

The behavioral results are in line with the literature on the DLB cognitive profile and confirm the existence of attention and processing speed impairment. Interestingly, VBM analysis revealed the involvement of the basal ganglia, in particular, the left caudate nucleus, which is part of the attention cerebral network, suggesting an important role of this structure for attentional processing speed. This also suggests the clinical implication of damage in this region relatively early in the course of the disease.
Recurrent giant fibroadenomas with transformation to cystosarcoma phyllodes in a 17-year-old girl: a rare case report from Syria
Authors: Ismail Sawsan , Alaidi Sara , Jouni Sarah , Kassab Yahya , Al-Shehabi Zuheir
Published: December 2019

Background

Fibroadenoma is the most prevalent benign breast lesion that generally affects middle-aged women; it is rare in adolescents and younger children. The transformation into malignancy is not common. However, multiple recurrences of rapidly enlarging fibroadenomas suggest a high possibility of transforming into phyllodes tumors, which are uncommon fibroepithelial lesions that account for 0.3–0.5% of female breast tumors and typically present in premenopausal women.

Case presentation

We report a case of a 17-year-old Syrian girl who previously had three episodes of recurrence of multiple rapidly enlarging fibroadenomas in her left breast and underwent three operations for complete resection of the lesions. However, a few months later, she was readmitted with multiple large masses in the same breast, and pathological findings confirmed a surprising combination of multiple fibroadenomas for the fourth time with a malignant phyllodes tumor (cystosarcoma phyllodes). The patient underwent lumpectomies followed by adjuvant radiotherapy. Long-term follow-up was recommended.

Conclusion

Our patient had an extraordinary number of episodes of recurrence at a young age and a rare combination of malignant and benign lesions in the same breast with multiple recurrences. We present her unique, very challenging case with the aim of highlighting the importance of clinical correlation, detailed diagnosis, and careful follow-up.
Healthy, mtDNA-mutation free mesoangioblasts from mtDNA patients qualify for autologous therapy
Authors: van Tienen Florence , Zelissen Ruby , Timmer Erika , van Gisbergen Marike , Lindsey Patrick , Quattrocelli Mattia , Sampaolesi Maurilio , Mulder-den Hartog Elvira , de Coo Irenaeus , Smeets Hubert
Published: December 2019

Background

Myopathy and exercise intolerance are prominent clinical features in carriers of a point-mutation or large-scale deletion in the mitochondrial DNA (mtDNA). In the majority of patients, the mtDNA mutation is heteroplasmic with varying mutation loads between tissues of an individual. Exercise-induced muscle regeneration has been shown to be beneficial in some mtDNA mutation carriers, but is often not feasible for this patient group. In this study, we performed in vitro analysis of mesoangioblasts from mtDNA mutation carriers to assess their potential to be used as source for autologous myogenic cell therapy.

Methods

We assessed the heteroplasmy level of patient-derived mesoangioblasts, isolated from skeletal muscle of multiple carriers of different mtDNA point-mutations (n = 25). Mesoangioblast cultures with < 10% mtDNA mutation were further analyzed with respect to immunophenotype, proliferation capacity, in vitro myogenic differentiation potential, mitochondrial function, and mtDNA quantity.

Results

This study demonstrated that mesoangioblasts in half of the patients contained no or a very low mutation load (< 10%), despite a much higher mutation load in their skeletal muscle. Moreover, none of the large-scale mtDNA deletion carriers displayed the deletion in mesoangioblasts, despite high percentages in skeletal muscle. The mesoangioblasts with no or a very low mutation load (< 10%) displayed normal mitochondrial function, proliferative capacity, and myogenic differentiation capacity.

Conclusions

Our data demonstrates that in half of the mtDNA mutation carriers, their mesoangioblasts are (nearly) mutation free and can potentially be used as source for autologous cell therapy for generation of new muscle fibers without mtDNA mutation and normal mitochondrial function.
Melatonin promotes the BMP9-induced osteogenic differentiation of mesenchymal stem cells by activating the AMPK/β-catenin signalling pathway
Authors: Jiang Tianyuan , Xia Chao , Chen Xiaoting , Hu Yan , Wang Yan , Wu Jin , Chen Shuyan , Gao Yanhong
Published: December 2019

Background

Mesenchymal stem cells (MSCs) play a crucial role in maintaining the dynamic balance of bone metabolism. Melatonin may have a regulatory effect on bone metabolism by regulating the lineage commitment and differentiation signalling pathways of MSCs. Among the BMP families, the osteogenesis of BMP9 is considered to be one of the strongest in MSCs. Here, we explored whether melatonin and BMP9 act synergistically on MSC osteogenic differentiation.

Methods

The C3H10T1/2 osteogenic differentiation function induced by melatonin synergizes with BMP9, as detected by the expression of osteogenic markers at different periods. The result was further confirmed by foetal limb explant culture and in vivo stem cell implantation experiments. The effects of the AMPK/β-catenin pathway on the osteogenic differentiation of C3H10T1/2 cells were evaluated by Western blotting.

Results

Melatonin combined with BMP9 significantly enhanced the expression of osteogenic markers at different periods in C3H10T1/2 cells, effectively enhancing BMP9-induced bone formation in cultured foetal explants and ectopic bone formation in vivo in stem cell transplantation experiments. Melatonin increases the expression of BMP9 in C3H10T1/2 cells and induces Smad1/5/8 translocation from the cytoplasm to the nucleus. In addition, melatonin and BMP9 synergistically promote AMPK and β-catenin phosphorylation, which can be largely eliminated by AMPK siRNA pretreatment.

Conclusions

Melatonin and BMP9 in C3H10T1/2 cells synergistically promote osteogenic differentiation at least in part by activating the AMPK/β-catenin signalling pathway.
Antiviral activity of interleukin-11 as a response to porcine epidemic diarrhea virus infection
Authors: Li Yuchen , Wu Qingxin , Jin Yuxin , Yang Qian
Published: December 2019

Interleukin-11 (IL-11), a well-known anti-inflammatory factor, provides protection from intestinal epithelium damage caused by physical or chemical factors. However, little is known of the role of IL-11 during viral infections. In this study, IL-11 expression at mRNA and protein levels were found to be high in Vero cells and the jejunum of piglets during porcine epidemic diarrhea virus (PEDV) infection, while IL-11 expression was found to be positively correlated with the level of viral infection. Pretreatment with recombinant porcine IL-11 (pIL-11) was found to suppress PEDV replication in Vero E6 cells, while IL-11 knockdown promoted viral infection. Furthermore, pIL-11 was found to inhibit viral infection by preventing PEDV-mediated apoptosis of cells by activating the IL-11/STAT3 signaling pathway. Conversely, application of a STAT3 phosphorylation inhibitor significantly antagonized the anti-apoptosis function of pIL-11 and counteracted its inhibition of PEDV. Our data suggest that IL-11 is a newfound PEDV-inducible cytokine, and its production enhances the anti-apoptosis ability of epithelial cells against PEDV infection. The potential of IL-11 to be used as a novel therapeutic against devastating viral diarrhea in piglets deserves more attention and study.
Myricitrin exhibits anti-atherosclerotic and anti-hyperlipidemic effects in diet-induced hypercholesterolemic rats
Authors: Gao Jing , Liu Cuicui , Zhang Heping , Sun Zhen , Wang Rongmei
Published: December 2019

The present study investigated the anti-atherosclerotic potential of myricitrin in hypercholesterolemic rats. Rats were divided into the following groups: sham (standard food), control [1% high-cholesterol diet (HCD)], 1 μM myricitrin + 1% HCD, 10 μM myricitrin + 1% HCD, 100 μM myricitrin + 1% HCD, and the positive control (10 mg/kg body weight atorvastatin). The dose was given to rats via oral gavage for 45 consecutive days. Feeding of rats with 1% HCD caused substantial increases in the levels of LDL, cholesterol, and triglycerides (TG), while high-density lipoprotein (HDL) was reduced. However, rats supplemented with myricitrin had reduced levels of cholesterol, LDL, and TG to near-normal levels, whereas HDL was increased. Catalase, superoxide dismutase (SOD), glutathione peroxidase (Gpx), and reduced glutathione (GSH) levels were substantially reduced in the HCD-fed rats compared with sham rats. However, the rats supplemented with 100 μM myricitrin showed > 50% increases in these levels. Lipid peroxidation and reactive oxygen species (ROS) levels were reduced following myricitrin treatment. The aortic cell wall area was significantly increased by 14.5% in HCD-fed rats. However, rats supplemented with 1, 10, and 100 μM myricitrin showed significant reductions in the aortic cell wall area of 2.3%, 4%, and 27.5%, respectively. This is the first report of the anti-atherosclerotic and hypolipidemic effects of myricitrin in hypercholesterolemic rats. Myricitrin decreased the level of total serum cholesterol and the role of aortic atherosclerosis in hypercholesterolemic rats.
Beauveria bassiana for the simultaneous control of Aedes albopictus and Culex pipiens mosquito adults shows high conidia persistence and productivity
Authors: Lee Jin Yong , Woo Ra Mi , Choi Cheol Jun , Shin Tae Young , Gwak Won Seok , Woo Soo Dong
Published: December 2019

This study was conducted to determine the optimal entomopathogenic fungus for the simultaneous control of the adults of two mosquito species, Aedes albopictus and Culex pipiens. The pathogenicity and virulence against the two species of mosquitoes were evaluated by using 30 isolates of Beauveria bassiana, an entomopathogenic fungus isolated from Korea that has high thermotolerance and UV-B tolerance. Regarding pathogenicity, 23 isolates were pathogenic to Ae. albopictus and 12 isolates were pathogenic to Cx. pipiens; Ae. albopictus adults were more susceptible to B. bassiana than Cx. pipiens adults. Among the isolates, 6 isolates that were simultaneously pathogenic to the two species of mosquitoes were used to evaluate virulence and conidia productivity. B. bassiana CN6T1W2 and JN5R1W1 had higher virulence than the other isolates, and they were more virulent in Ae. albopictus than inCx. pipiens. The conidia productivity of B. bassiana JN5R1W1 on millet grain medium was higher than that of B. bassiana CN6T1W2. Based on these results, B. bassiana JN5R1W1 was selected as the most efficient isolate for the simultaneous control of the two mosquito species. B. bassiana JN5R1W1 can be used effectively in the development of fungal insecticides to simultaneously control Ae. albopictus and Cx. pipiens adults with similar distribution areas.
Evaluation of a class of NLFM radar signals
Authors: Alphonse Sebastian , Williamson Geoffrey A.
Published: December 2019

Signal design is an important component for good performance of radar systems. Here, the problem of determining a good radar signal with the objective of minimizing autocorrelation sidelobes is addressed, and the first comprehensive comparison of a range of signals proposed in the literature is conducted. The search is restricted to a set of nonlinear, frequency-modulated signals whose frequency function is monotonically nondecreasing and antisymmetric about the temporal midpoint. This set includes many signals designed for smaller sidelobes including our proposed odd polynomial frequency signal (OPFS) model and antisymmetric time exponentiated frequency modulated (ATEFM) signal model. The signal design is optimized based on autocorrelation sidelobe levels with constraints on the autocorrelation mainlobe width and leakage of energy outside the allowed bandwidth, and we compare our optimized design with the best signal found from parameterized signal model classes in the literature. The quality of the overall best such signal is assessed through comparison to performance of a large number of randomly generated signals from within the search space. From this analysis, it is found that the OPFS model proposed in this paper outperforms all other contenders for most combinations of the objective functions and is expected to be better than nearly all signals within the entire search set.
Modeling the UE-perceived cellular network performance following a controller-based approach
Authors: Mendoza Jessica , Palacios David , de-la-Bandera Isabel , Baena Eduardo , Khatib Emil J , Barco Raquel
Published: December 2019

During the last few years, mobile communication networks have experienced a huge evolution. This evolution culminates with the arrival of the fifth generation (5G) of mobile communication networks. As a result, the complexity of network management tasks has been increasing and the need to use automatic management algorithms has been demonstrated. However, many mobile network operators (MNOs) are reluctant to evaluate these algorithms in their networks. To address this issue, in this paper, a modeling approach is proposed. In this sense, the behavior of a commercial network, as it is perceived by user equipments (UEs), has been replicated in a research testbed using a three-step modeling process. The first step consists on performing a measurement campaign in several external networks. The second step is composed of the measurement campaign result analysis and the classification of the results in different types of scenarios. Finally, the third step is related to the application of a modeling algorithm in a research testbed. In order to perform the last step, the use of a method based on a controller is proposed. The modeling process presented in this paper allows to replicate the network behavior from users located in different areas and with different conditions point of view. Moreover, the use of a testbed environment can help to avoid downtime in commercial networks caused by possible algorithm bugs.
Personalized project recommendations: using reinforcement learning
Authors: Qi Faxin , Tong Xiangrong , Yu Lei , Wang Yingjie
Published: December 2019

With the development of the Internet and the progress of human-centered computing (HCC), the mode of man-machine collaborative work has become more and more popular. Valuable information in the Internet, such as user behavior and social labels, is often provided by users. A recommendation based on trust is an important human-computer interaction recommendation application in a social network. However, previous studies generally assume that the trust value between users is static, unable to respond to the dynamic changes of user trust and preferences in a timely manner. In fact, after receiving the recommendation, there is a difference between actual evaluation and expected evaluation which is correlated with trust value. Based on the dynamics of trust and the changing process of trust between users, this paper proposes a trust boost method through reinforcement learning. Recursive least squares (RLS) algorithm is used to learn the dynamic impact of evaluation difference on user’s trust. In addition, a reinforcement learning method Deep Q-Learning (DQN) is studied to simulate the process of learning user’s preferences and boosting trust value. Experiments indicate that our method applied to recommendation systems could respond to the changes quickly on user’s preferences. Compared with other methods, our method has better accuracy on recommendation.
Estimation of unknown function of nonlinear weakly singular integral inequalities of Gronwall–Bellman–Pachpatte type
Authors: Yan Ran , Meng Fanwei
Published: December 2019

Some new nonlinear weakly singular integral inequalities of Gronwall–Bellman–Pachpatte type are given. The estimations of unknown functions are obtained by analysis techniques. These estimates are very significant tools in the study of differential-integral equations.
Sharp thresholds of blow-up and global existence for the Schrödinger equation with combined power-type and Choquard-type nonlinearities
Authors: Wang Yongbin , Feng Binhua
Published: December 2019

In this paper, we consider the sharp thresholds of blow-up and global existence for the nonlinear Schrödinger–Choquard equation
$$ i\psi _{t}+\Delta \psi =\lambda _{1} \vert \psi \vert ^{p_{1}}\psi +\lambda _{2}\bigl(I _{\alpha } \ast \vert \psi \vert ^{p_{2}}\bigr) \vert \psi \vert ^{p_{2}-2}\psi . $$iψt+Δψ=λ1|ψ|p1ψ+λ2(Iα∗|ψ|p2)|ψ|p2−2ψ. We derive some finite time blow-up results. Due to the failure of this equation to be scale invariant, we obtain some sharp thresholds of blow-up and global existence by constructing some new estimates. In particular, we prove the global existence for this equation with critical mass in the $L^{2}$L2-critical case. Our obtained results extend and improve some recent results.
Stoichiometric knife-edge model on discrete time scale
Authors: Chen Ming , Asik Lale , Peace Angela
Published: December 2019

Ecological stoichiometry is the study of the balance of multiple elements in ecological interactions and processes (Sterner and Elser in Ecological Stoichiometry: The Biology of Elements from Molecules to the Biosphere, 2002). Modeling under this framework enables us to investigate the effect nutrient content on organisms whether the imbalance involves insufficient or excess nutrient content. This phenomenon is called the “stoichiometric knife-edge”. In this paper, a discrete-time predator–prey model that captures this phenomenon is established and qualitatively analyzed. We systematically expound the similarities and differences between our discrete model and the corresponding continuous analog. Theoretical and numerical analyses show that while the discrete and continuous models share many properties, differences also exist. Under certain parameter sets, the models exhibit qualitatively different dynamics. While the continuous model shows limit cycle, Hopf bifurcation, and saddle-node bifurcation, the discrete-time model exhibits richer dynamical behaviors, such as chaos. By comparing the dynamics of the continuous and discrete model, we can conclude that stoichiometric effects of low food quality on predators are robust to the discretization of time. This study can possibly serve as an example for pointing to the importance of time scale in ecological modeling.
An overview of behavioral, physiological, and environmental sensors used in animal biotelemetry and biologging studies
Authors: Whitford Malachi , Klimley A. Peter
Published: December 2019

Background

The ability to remotely monitor the behavior of animals and their interactions with their environment has revolutionized how ecologists conduct studies. The creative use and placement of sensors on both biologging and biotelemetric platforms can greatly expand the amount of information that can be garnered from ecological studies.

Results

Sophisticated transmitters and data loggers, which once were built by the biologists that used them, are available off the shelf from many commercial manufacturers. The ability to purchase a wide variety of electronic tags has allowed for a wider adoption of electronic tags across ecology, but has resulted in many biologists utilizing them with little understanding of how they function. The purpose of this review is to provide a reader-friendly description of the many sensors available to monitor the behavior, physiology, and environment of both terrestrial and aquatic animals. Our approach here is firstly to describe the electrical and mechanical principles behind each type of sensor and secondly to present one or two classic examples of how they have been used to provide insights into the biology of species from a diversity of taxa. Behavioral sensors that record the speed, acceleration, tilt angle, and direction of movement of an animal as well as its swimming depth or flight altitude will be described. Additional sensors are mentioned that detect feeding and spawning behavior as well as the proximity to conspecifics, prey, and predators. Physiological sensors will be described that monitor muscular, sensory, brain, gastric activity as well as body temperature, and sound production. Environmental sensors will be described that measure irradiance, dissolved oxygen, and magnetic field intensity. It is our hope that this review serves as springboard for biologists to develop innovative ways to learn more about their subjects using the myriad sensors that are available today, and the exciting new sensors to be developed in the future.
Patients with chronic pain: evaluating depression and their quality of life in a single center study in Greece
Authors: Rapti Ekaterini , Damigos Dimitrios , Apostolara Paraskevi , Roka Vasiliki , Tzavara Chara , Lionis Christos
Published: December 2019

Background

Chronic pain constitutes one of the most common reasons for seeking health care services and may even lead to disability. Chronic pain has been associated with depression and deterioration of the quality of life. The aim of our study is to outline the burden of chronic pain in the context of a primary health care (PHC) setting in Greece and to investigate its association with depression and quality of life.

Methods

A cross-sectional study was conducted from September 2016 to November 2016. The subjects of the study comprised 200 individuals who visited the regional medical center of Ag. Theodoroi, Greece. The collected data were from a representative sample of 200 adults and included demographic data, social and medical history, presence and characteristics of chronic pain and questions from three questionnaires for the assessment of pain (BPI- short form), the investigation of depression (PHQ-9) and the evaluation of the quality of life (EuroQ-5D) validated in Greek language. Multiple regression analysis was used in order to find associated factors with quality of life, depression and chronic pain.

Results

A percentage of 56.8% of the participants, the majority of whom (62%) were women, reported chronic pain. Among individuals with pain, lower back area was the most common location. Based on the given questionnaire, depression was detected in 22. 5% of the participants who claimed chronic pain. Regression analyses revealed that women and respondents with chronic mental disorders like depression and anxiety had significantly higher scores on the pain scale and suffered pain which had a greater impact on their daily activities. According to regression analysis decreased quality of life was expressed by women, as well as participants with a chronic mental disorder. A significant reverse correlation emerged between the quality of life, depression and pain scales.

Conclusion

Chronic pain, as it has been studied within this PHC setting, is a common health care problem. Individuals who had experienced chronic pain and depression had a lower health-related quality of life.
Size-dependent tradeoffs in seasonal freshwater environments facilitate differential salmonid migration
Authors: Dermond Philip , Melián Carlos J. , Brodersen Jakob
Published: December 2019

Background

Seasonal spatio-temporal variation in habitat quality and abiotic conditions leads to animals migrating between different environments around the world. Whereas mean population timing of migration is often fairly well understood, explanations for variation in migratory timing within populations are often lacking. Condition-dependent tradeoffs may be an understudied mechanism that can explain this differential migration. While fixed condition-specific thresholds have been identified in earlier work on ontogenetic niche shifts, they are rare in differential migration, suggesting that thresholds in such systems can shift based on temporally variable environmental conditions.

Methods

We introduced a model based on size-specific tradeoffs between migration and growth in seasonal environments. We focused on optimal migratory timing for first-time migrants with no knowledge of an alternative habitat, which is a crucial stage in the life history of migratory salmonids. We predicted that optimal timing would occur when individuals move from their natal habitats based on a seasonally variable ratio of predation and growth. When the ratio becomes slightly more favorable in the alternative habitat, migratory movement can occur. As it keeps shifting throughout the season, the threshold for migration is variable, allowing smaller individuals to move at later dates. We compared our model predictions to empirical data on 3 years of migratory movement of more than 800 juvenile trout of varying size from natal to feeding habitat.

Results

Both our model and empirical data showed that large individuals, which are assumed to have a lower predation risk in the migratory habitat, move earlier in the season than smaller individuals, whose predicted predation-to-growth ratio shifted to being favorable only later in the migratory season. Our model also predicted that the observed difference in migratory timing between large and small migrants occurred most often at low values of growth differential between the two habitats, suggesting that it was not merely high growth potential but rather the tradeoff between predation and growth that shaped differential migration patterns.

Conclusions

We showed the importance of considering condition-specific tradeoffs for understanding temporal population dynamics in spatially structured landscapes. Rather than assuming a fixed threshold, which appears to be absent based on previous work on salmonids, we showed that the body-size threshold for migration changed temporally throughout the season. This allowed increasingly smaller individuals to migrate when growth conditions peaked in the migratory habitat. Our model illuminates an understudied aspect of predation as part of a condition-dependent tradeoff that shapes migratory patterns, and our empirical data back patterns predicted by this model.