bluuebunny/crossref_metadata_2025 huggingface dataset:
Unveiling the Role of Oxidative Stress in Cochlear Hair Cell Death: Prospective Phytochemical Therapeutics against Sensorineural Hearing Loss
Authors: Gill Nicholas B. , Dowker-Key Presley D. , Hedrick Mark , Bettaieb Ahmed
Published: April 2024

Hearing loss represents a multifaceted and pervasive challenge that deeply impacts various aspects of an individual’s life, spanning psychological, emotional, social, and economic realms. Understanding the molecular underpinnings that orchestrate hearing loss remains paramount in the quest for effective therapeutic strategies. This review aims to expound upon the physiological, biochemical, and molecular aspects of hearing loss, with a specific focus on its correlation with diabetes. Within this context, phytochemicals have surfaced as prospective contenders in the pursuit of potential adjuvant therapies. These compounds exhibit noteworthy antioxidant and anti-inflammatory properties, which hold the potential to counteract the detrimental effects induced by oxidative stress and inflammation—prominent contributors to hearing impairment. Furthermore, this review offers an up-to-date exploration of the diverse molecular pathways modulated by these compounds. However, the dynamic landscape of their efficacy warrants recognition as an ongoing investigative topic, inherently contingent upon specific experimental models. Ultimately, to ascertain the genuine potential of phytochemicals as agents in hearing loss treatment, a comprehensive grasp of the molecular mechanisms at play, coupled with rigorous clinical investigations, stands as an imperative quest.
Synthesis of Estrone Heterodimers and Evaluation of Their In Vitro Antiproliferative Activity
Authors: Bózsity Noémi , Nagy Viktória , Szabó Johanna , Pálházi Balázs , Kele Zoltán , Resch Vivien , Paragi Gábor , Zupkó István , Minorics Renáta , Mernyák Erzsébet
Published: April 2024

Directed structural modifications of natural products offer excellent opportunities to develop selectively acting drug candidates. Natural product hybrids represent a particular compound group. The components of hybrids constructed from different molecular entities may result in synergic action with diminished side effects. Steroidal homo- or heterodimers deserve special attention owing to their potentially high anticancer effect. Inspired by our recently described antiproliferative core-modified estrone derivatives, here, we combined them into heterodimers via Cu(I)-catalyzed azide–alkyne cycloaddition reactions. The two trans-16-azido-3-(O-benzyl)-17-hydroxy-13α-estrone derivatives were reacted with 3-O-propargyl-D-secoestrone alcohol or oxime. The antiproliferative activities of the four newly synthesized dimers were evaluated against a panel of human adherent gynecological cancer cell lines (cervical: Hela, SiHa, C33A; breast: MCF-7, T47D, MDA-MB-231, MDA-MB-361; ovarian: A2780). One heterodimer (12) exerted substantial antiproliferative activity against all investigated cell lines in the submicromolar or low micromolar range. A pronounced proapoptotic effect was observed by fluorescent double staining and flow cytometry on three cervical cell lines. Additionally, cell cycle blockade in the G2/M phase was detected, which might be a consequence of the effect of the dimer on tubulin polymerization. Computational calculations on the taxoid binding site of tubulin revealed potential binding of both steroidal building blocks, mainly with hydrophobic interactions and water bridges.
Preventive Effect of the Total Polyphenols from Nymphaea candida on Sepsis-Induced Acute Lung Injury in Mice via Gut Microbiota and NLRP3, TLR-4/NF-κB Pathway
Authors: Li Chenyang , Qi Xinxin , Xu Lei , Sun Yuan , Chen Yan , Yao Yuhan , Zhao Jun
Published: April 2024

This study aimed to investigate the preventive effects of the total polyphenols from Nymphaea candida (NCTP) on LPS-induced septic acute lung injury (ALI) in mice and its mechanisms. NCTP could significantly ameliorate LPS-induced lung tissue pathological injury in mice as well as lung wet/dry ratio and MPO activities (p < 0.05). NCTP could significantly decrease the blood leukocyte, neutrophil, monocyte, basophil, and eosinophil amounts and LPS contents in ALI mice compared with the model group (p < 0.05), improving lymphocyte amounts (p < 0.05). Moreover, compared with the model group, NCTP could decrease lung tissue TNF-α, IL-6, and IL-1β levels (p < 0.05) and downregulate the protein expression of TLR4, MyD88, TRAF6, IKKβ, IκB-α, p-IκB-α, NF-κB p65, p-NF-κB p65, NLRP3, ASC, and Caspase1 in lung tissues (p < 0.05). Furthermore, NCTP could inhibit ileum histopathological injuries, restoring the ileum tight junctions by increasing the expression of ZO-1 and occludin. Simultaneously, NCTP could reverse the gut microbiota disorder, restore the diversity of gut microbiota, increase the relative abundance of Clostridiales and Lachnospiraceae, and enhance the content of SCFAs (acetic acid, propionic acid, and butyric acid) in feces. These results suggested that NCTP has preventive effects on septic ALI, and its mechanism is related to the regulation of gut microbiota, SCFA metabolism, and the TLR-4/NF-κB and NLRP3 pathways.
KYNA Ameliorates Glutamate Toxicity of HAND by Enhancing Glutamate Uptake in A2 Astrocytes
Authors: Chen Jie , Zou Jinhu , Huang Pengwei , Gao Xuefeng , Lun Jingxian , Li Yubin , Gong Zelong , Cao Hong
Published: April 2024

Reactive astrocytes are key players in HIV-associated neurocognitive disorders (HAND), and different types of reactive astrocytes play opposing roles in the neuropathologic progression of HAND. A recent study by our group found that gp120 mediates A1 astrocytes (neurotoxicity), which secrete proinflammatory factors and promote HAND disease progression. Here, by comparing the expression of A2 astrocyte (neuroprotective) markers in the brains of gp120 tgm mice and gp120+/α7nAChR−/− mice, we found that inhibition of alpha 7 nicotinic acetylcholine receptor (α7nAChR) promotes A2 astrocyte generation. Notably, kynurenine acid (KYNA) is an antagonist of α7nAChR, and is able to promote the formation of A2 astrocytes, the secretion of neurotrophic factors, and the enhancement of glutamate uptake through blocking the activation of α7nAChR/NF-κB signaling. In addition, learning, memory and mood disorders were significantly improved in gp120 tgm mice by intraperitoneal injection of kynurenine (KYN) and probenecid (PROB). Meanwhile, the number of A2 astrocytes in the mouse brain was significantly increased and glutamate toxicity was reduced. Taken together, KYNA was able to promote A2 astrocyte production and neurotrophic factor secretion, reduce glutamate toxicity, and ameliorate gp120-induced neuropathological deficits. These findings contribute to our understanding of the role that reactive astrocytes play in the development of HAND pathology and provide new evidence for the treatment of HAND via the tryptophan pathway.
Effects of Mild Closed-Head Injury and Subanesthetic Ketamine Infusion on Microglia, Axonal Injury, and Synaptic Density in Sprague–Dawley Rats
Authors: Boese Martin , Berman Rina Y. , Qiu Jennifer , Spencer Haley F. , Radford Kennett D. , Choi Kwang H.
Published: April 2024

Mild traumatic brain injury (mTBI) affects millions of people in the U.S. Approximately 20–30% of those individuals develop adverse symptoms lasting at least 3 months. In a rat mTBI study, the closed-head impact model of engineered rotational acceleration (CHIMERA) produced significant axonal injury in the optic tract (OT), indicating white-matter damage. Because retinal ganglion cells project to the lateral geniculate nucleus (LGN) in the thalamus through the OT, we hypothesized that synaptic density may be reduced in the LGN of rats following CHIMERA injury. A modified SEQUIN (synaptic evaluation and quantification by imaging nanostructure) method, combined with immunofluorescent double-labeling of pre-synaptic (synapsin) and post-synaptic (PSD-95) markers, was used to quantify synaptic density in the LGN. Microglial activation at the CHIMERA injury site was determined using Iba-1 immunohistochemistry. Additionally, the effects of ketamine, a potential neuroprotective drug, were evaluated in CHIMERA-induced mTBI. A single-session repetitive (ssr-) CHIMERA (3 impacts, 1.5 joule/impact) produced mild effects on microglial activation at the injury site, which was significantly enhanced by post-injury intravenous ketamine (10 mg/kg) infusion. However, ssr-CHIMERA did not alter synaptic density in the LGN, although ketamine produced a trend of reduction in synaptic density at post-injury day 4. Further research is necessary to characterize the effects of ssr-CHIMERA and subanesthetic doses of intravenous ketamine on different brain regions and multiple time points post-injury. The current study demonstrates the utility of the ssr-CHIMERA as a rodent model of mTBI, which researchers can use to identify biological mechanisms of mTBI and to develop improved treatment strategies for individuals suffering from head trauma.
Design of Dyes Based on the Quinoline or Quinoxaline Skeleton towards Visible Light Photoinitiators
Authors: Pyszka Ilona , Jędrzejewska Beata
Published: April 2024

Dyes based on quinoline and quinoxaline skeletons were designed for application as visible light photoinitiators. The obtained compounds absorb electromagnetic radiation on the border between ultraviolet and visible light, which allows the use of dental lamps as light sources during the initiation of the photopolymerization reaction. Their another desirable feature is the ability to create a long-lived excited state, which enables the chain reaction to proceed through the mechanism of intermolecular electron transfer. In two-component photoinitiating systems, in the presence of an electron donor or a hydrogen atom donor, the synthesized compounds show excellent abilities to photoinitiate the polymerization of acrylates. In control tests, the efficiency of photopolymerization using modified quinoline and quinoxaline derivatives is comparable to that obtained using a typical, commercial photoinitiator for dentistry, camphorquinone. Moreover, the use of the tested compounds requires a small amount of photoinitiator (only 0.04% by weight) to initiate the reaction. The research also showed a significant acceleration of the photopolymerization process and shortening of the reaction time. In practice, this means that the new two-component initiating systems can be used in much lower concentrations without slowing down the speed of obtaining polymer materials. It is worth emphasizing that these two features of the new initiating system allow for cost reduction by reducing financial outlays on both materials (photoinitiators) and electricity.
Structure, Functions, and Implications of Selected Lipocalins in Human Disease
Authors: Chandrasekaran Preethi , Weiskirchen Sabine , Weiskirchen Ralf
Published: April 2024

The lipocalin proteins are a large family of small extracellular proteins that demonstrate significant heterogeneity in sequence similarity and have highly conserved crystal structures. They have a variety of functions, including acting as carrier proteins, transporting retinol, participating in olfaction, and synthesizing prostaglandins. Importantly, they also play a critical role in human diseases, including cancer. Additionally, they are involved in regulating cellular homeostasis and immune response and dispensing various compounds. This comprehensive review provides information on the lipocalin family, including their structure, functions, and implications in various diseases. It focuses on selective important human lipocalin proteins, such as lipocalin 2 (LCN2), retinol binding protein 4 (RBP4), prostaglandin D2 synthase (PTGDS), and α1-microglobulin (A1M).
The Suppression of the Epithelial to Mesenchymal Transition in Prostate Cancer through the Targeting of MYO6 Using MiR-145-5p
Authors: Armstrong Lee , Willoughby Colin E. , McKenna Declan J.
Published: April 2024

Aberrant expression of miR-145-5p has been observed in prostate cancer where is has been suggested to play a tumor suppressor role. In other cancers, miR-145-5p acts as an inhibitor of epithelial-to-mesenchymal transition (EMT), a key molecular process for tumor progression. However, the interaction between miR-145-5p and EMT remains to be elucidated in prostate cancer. In this paper the link between miR-145-5p and EMT in prostate cancer was investigated using a combination of in silico and in vitro analyses. miR-145-5p expression was significantly lower in prostate cancer cell lines compared to normal prostate cells. Bioinformatic analysis of The Cancer Genome Atlas prostate adenocarcinoma (TCGA PRAD) data showed significant downregulation of miR-145-5p in prostate cancer, correlating with disease progression. Functional enrichment analysis significantly associated miR-145-5p and its target genes with EMT. MYO6, an EMT-associated gene, was identified and validated as a novel target of miR-145-5p in prostate cancer cells. In vitro manipulation of miR-145-5p levels significantly altered cell proliferation, clonogenicity, migration and expression of EMT-associated markers. Additional TCGA PRAD analysis suggested miR-145-5p tumor expression may be useful predictor of disease recurrence. In summary, this is the first study to report that miR-145-5p may inhibit EMT by targeting MYO6 in prostate cancer cells. The findings suggest miR-145-5p could be a useful diagnostic and prognostic biomarker for prostate cancer.
Using ML to Predict User Satisfaction with ICT Technology for Educational Institution Administration
Authors: Almaghrabi Hamad , Soh Ben , Li Alice
Published: April 2024

Effective and efficient use of information and communication technology (ICT) systems in the administration of educational organisations is crucial to optimise their performance. Earlier research on the identification and analysis of ICT users’ satisfaction with administration tasks in education is limited and inconclusive, as they focus on using ICT for nonadministrative tasks. To address this gap, this study employs Artificial Intelligence (AI) and machine learning (ML) in conjunction with a survey technique to predict the satisfaction of ICT users. In doing so, it provides an insight into the key factors that impact users’ satisfaction with the ICT administrative systems. The results reveal that AI and ML models predict ICT user satisfaction with an accuracy of 94%, and identify the specific ICT features, such as usability, privacy, security, and Information Technology (IT) support as key determinants of satisfaction. The ability to predict user satisfaction is important as it allows organisations to make data-driven decisions on improving their ICT systems to better meet the needs and expectations of users, maximising labour effort while minimising resources, and identifying potential issues earlier. The findings of this study have important implications for the use of ML in improving the administration of educational institutions and providing valuable insights for decision-makers and developers.
Transportation System and the Improvement of Urban Vehicular Flow in the District of Huánuco-Perú 2022
Authors: Verastegui Yessica Julia , Esenarro Doris
Published: April 2024

The objective of this research is to propose a public transport reorganization system that allows the improvement of urban vehicle flow. The lack of adequate transportation infrastructure and the existing disorder in the services provided by collective car, Microbus, Rural Public Transportation Van (Combi), Coaster, and mototaxis generate congestion in public transportation, especially during peak hours, resulting in environmental and noise pollution. The research was structured into four stages: data collection on the public and private transportation network, importing and creating the transportation network in the urban area of the Huánuco district, zoning and connectivity of the study area, and finally, creating the origin/destination (O/D) matrix for public transportation, supported by digital tools (ArcGIS 10.5, AutoCAD 2018, Excel 2017). To meet the demand of 135,343 passengers from South to North and 118,958 from North to South, the proposal includes establishing one main route and seven feeder routes, requiring 422 buses and road infrastructure, as depicted in the proposal This system will have exclusive lanes to operate the Mass Transit System, allowing it to accommodate 59% of users who prefer using public transportation. This proposal aims to offer an efficient and high-quality transportation system.
Insecticide Susceptibilities and Enzyme Activities of Four Stink Bug Populations in Mississippi, USA
Authors: Du Yuzhe , Scheibener Shane , Zhu Yu-Cheng , Allen K. Clint , Reddy Gadi V. P.
Published: April 2024

In Mississippi, the Pentatomidae complex infesting soybean is primarily composed of Euschistus servus, Nezara viridula, Chinavia hilaris, and Piezodorus guildinii. This study employed spray bioassays to evaluate the susceptibilities of these stink bugs to seven commonly used formulated insecticides: oxamyl, acephate, bifenthrin, λ-cyhalothrin, imidacloprid, thiamethoxam, and sulfoxaflor. Stinks bugs were collected from soybeans in Leland, MS, USA during 2022 and 2023, as well as from wild host plants in Clarksdale, MS. There was no significant difference in the susceptibility of C. hilaris to seven insecticides between two years, whereas P. guildinii showed slightly increased susceptibility to neonicotinoids in 2023. Among all four stink bug species, susceptibility in 2022 was ranked as P. guildinii ≤ C. hilaris ≈ N. viridula, while in 2023, it was ranked as P. guildinii ≤ C. hilaris ≤ E. Servus. Additionally, populations of E. servus and P. guildinii collected from Clarksdale exhibited high tolerance to pyrethroids and neonicotinoids. Moreover, populations of E. servus and P. guildinii from SIMRU-2022 and Clarksdale-2023 showed elevated esterase and cytochrome P450 activity, respectively. These findings from spray bioassays and enzyme activity analyses provide a baseline for monitoring insecticide resistance in Pentatomidae and can guide insecticide resistance management strategies for Mississippi soybean.
Body Size of Female Strepsipteran Parasites (Strepsiptera, Xenidae, Xenos) Depends on Several Key Factors in a Vespine Wasp (Hymenoptera, Vespidae, Vespa)
Authors: Kudô Kazuyuki , Oyaizu Wataru , Kusama Rikako , Yamaguchi Yuki , Koji Shinsaku
Published: April 2024

Parasite growth in hosts depends on their hosts’ nutritional status. This study examined whether the body size of the strepsipteran parasite Xenos oxyodontes, which parasitizes the eusocial wasp Vespa analis, is affected by several key factors, including host body size. We collected V. analis using bait traps for three years in Niigata, Japan, and evaluated the number of male and female X. oxyodontes parasites throughout the seasons. A total of 185 female parasites were collected, and their cephalothorax widths were measured. The widths of female parasites did not statistically vary among seasons and were positively correlated with the head widths of female host wasps but negatively correlated with the number of parasites per host wasp. In addition, we examined whether the reproductive conditions of post-hibernation solitary queens affected the cephalothorax width of female parasites. The widths of the female parasites were greater when the queens had less-developed ovaries. These results suggested that nutrient availability by female parasites depends on the host wasp and competition with conspecific individuals.
The Expected Dynamics for the Extreme Wind and Wave Conditions at the Mouths of the Danube River in Connection with the Navigation Hazards
Authors: Răileanu Alina Beatrice , Rusu Liliana , Marcu Andra , Rusu Eugen
Published: April 2024

The entrance in the Sulina channel in the Black Sea is the target area of this study. This represents the southern gate of the seventh Pan-European transport corridor, and it is usually subjected to high navigation traffic. The main objective of the work is to provide a more comprehensive picture concerning the past and future expected dynamics of the environmental matrix in this coastal area, including especially the extreme wind and wave conditions in connection with the possible navigation risks. The methodology considered assumes analyses performed at three different levels. First, an analysis of some in situ measurements at the zero-kilometer point of the Danube is carried out for the 15-year period of 2009–2023. Together with the maximum wind speed and the maximum value of the wind gusts, the water level variation was analyzed at this point. As a second step, the analysis is based on wind speed data provided by regional climate models. Two periods, each spanning 30 years, are considered. These are the recent past (1976–2005), when comparisons with ERA5 reanalysis data were also performed, and the near future (2041–2070), when two different models and three climate scenarios were considered. The focus was on the extreme wind speed values, performing comparisons between the past and future expected extreme winds. Finally, the third analysis is related to the wave conditions. Thus, using as a forcing factor each of the wind fields that was previously analyzed, simulations employing a spectral wave model were carried out. The wave modeling system was focused using three different computational domains with increasing resolution towards the target area, and the nearshore wave conditions were evaluated. The results show that both the extreme wind and wave conditions are expected to slightly increase in the future. Especially in the wintertime, strong wind fields are often expected in this area, with wind gusts exceeding more than 70% of the hourly average wind velocity. With regard to the waves, due to the complex nearshore phenomena, considerable enhancements in terms of significant wave heights are induced, and there is also an elevated risk of the occurrence of rogue waves. This work is still ongoing, and taking into account the high navigation risks highlighted, the next step would be to elaborate the risk assessment of severe shipping conditions, particularly related to the likelihood or probability of adverse conditions with the potential of generating hazardous situations in this coastal environment.
Correlated Decision Fusion Accompanied with Quality Information on a Multi-Band Pixel Basis for Land Cover Classification
Authors: Papadopoulos Spiros , Koukiou Georgia , Anastassopoulos Vassilis
Published: April 2024

Decision fusion plays a crucial role in achieving a cohesive and unified outcome by merging diverse perspectives. Within the realm of remote sensing classification, these methodologies become indispensable when synthesizing data from multiple sensors to arrive at conclusive decisions. In our study, we leverage fully Polarimetric Synthetic Aperture Radar (PolSAR) and thermal infrared data to establish distinct decisions for each pixel pertaining to its land cover classification. To enhance the classification process, we employ Pauli’s decomposition components and land surface temperature as features. This approach facilitates the extraction of local decisions for each pixel, which are subsequently integrated through majority voting to form a comprehensive global decision for each land cover type. Furthermore, we investigate the correlation between corresponding pixels in the data from each sensor, aiming to achieve pixel-level correlated decision fusion at the fusion center. Our methodology entails a thorough exploration of the employed classifiers, coupled with the mathematical foundations necessary for the fusion of correlated decisions. Quality information is integrated into the decision fusion process, ensuring a comprehensive and robust classification outcome. The novelty of the method is its simplicity in the number of features used as well as the simple way of fusing decisions.
Influence of the Hot-Top Thermal Regime on the Severity and Extent of Macrosegregation in Large-Size Steel Ingots
Authors: Ghodrati Neda , Champliaud Henri , Morin Jean-Benoit , Jahazi Mohammad
Published: April 2024

The influence of hot-top designs with different heat capacities on the distribution of positive and negative macrosegregation was investigated on a 12 metric tonne (MT) cast ingot made using Cr-Mo low-alloy steel. The three-dimensional finite element modeling code THERCAST® was used to simulate the thermo-mechanical phenomena associated with the solidification process, running from filling the mold until complete solidification. The model was validated on an industrial-scale ingot and then utilized to evaluate the influence of the thermal history of the hot-top, a crucial component in the cast ingot setup. This assessment aimed to comprehend changes in solidification time, temperature, and heat flux—all of which contribute to the determination of macrosegregation severity. The results showed that preheating the hot-top had a minor effect on solidification time, while modifications of thermal conductivity in the hot-top region increased the solidification time by 31%, thereby significantly affecting the macrosegregation patterns. The results are discussed and interpreted in terms of the fundamental mechanisms governing the kinetics of solidification and macrosegregation phenomena.
Data on Ocrelizumab Treatment Collected by MS Patients in Germany Using Brisa App
Authors: Papukchieva Steffeni , Kahn Maria , Eberl Markus , Friedrich Benjamin , Joschko Natalie , Ziemssen Tjalf
Published: April 2024

Background: With a rising number of multiple sclerosis (MS) cases and increasing pressure on health systems, digital companion apps like Brisa, designed specifically for people with MS, can play an important role in the patient journey. These apps enable the collection of real-time longitudinal data that are critical to our understanding of the pathophysiology and progression of MS. Methods: This retrospective, descriptive analysis consists of data from Brisa users who registered between 6 August 2021 and 8 September 2022. Of the unique users, 37.7% (n = 1593) fulfilled the inclusion criteria including information about medication and demographics and tracked one or more symptoms and/or patient-reported outcomes. Users were classified as moderate-efficacy treatment users, high-efficacy treatment users and ocrelizumab users, and the reporting frequency and scores of symptoms and patient-reported outcomes were analyzed. Results: The largest cohort of Brisa users (405) reported treatment with ocrelizumab and were mostly diagnosed 2–5 years before the survey. The most reported MS symptoms were similar between OUs (ocrelizumab users), HETUs (high-efficacy treatment users) and METUs (moderate-efficacy treatment users). OUs on average reported symptoms and answered questionnaires more frequently. Baseline scores between HETUs and OUs were similar, whereas baseline scores of METUs were slightly lower in comparison. In a further analysis of OUs, disability scores increased with age; users aged 26–45 years had higher pain scores than 18–25-year-olds. No significant differences were found in quality of life, bowel control and vision between age groups. Conclusion: These findings show that the characteristics of the Brisa cohort are similar to the results of other studies and registries and can provide a representative overview of everyday disease management. Thereby, these results can bridge the gap between clinical research and real patient experience, but they also raise new questions, such as how often the hard-and-early therapy approach is already used and whether baseline characteristics and reasons for choosing a particular treatment contribute to the different outcomes over time. Answering these questions requires further research and analysis.
Prediction of Acute Cardiac Rejection Based on Gene Expression Profiles
Authors: Abdrakhimov Bulat , Kayewa Emmanuel , Wang Zhiwei
Published: April 2024

Acute cardiac rejection remains a significant challenge in the post-transplant period, necessitating meticulous monitoring and timely intervention to prevent graft failure. Thus, the goal of the present study was to identify novel biomarkers involved in acute cardiac rejection, paving the way for personalized diagnostic, preventive, and treatment strategies. A total of 809 differentially expressed genes were identified in the GSE150059 dataset. We intersected genes selected by analysis of variance, recursive feature elimination, least absolute shrinkage and selection operator, and random forest classifier to identify the most relevant genes involved in acute cardiac rejection. Thus, HCP5, KLRD1, GZMB, PLA1A, GNLY, and KLRB1 were used to train eight machine learning models: random forest, logistic regression, decision trees, support vector machines, gradient boosting machines, K-nearest neighbors, XGBoost, and neural networks. Models were trained, tested, and validated on the GSE150059 dataset (MMDx-based diagnosis of rejection). Eight algorithms achieved great performance in predicting acute cardiac rejection. However, all machine learning models demonstrated poor performance in two external validation sets that had rejection diagnosis based on histology: merged GSE2596 and GSE4470 dataset and GSE9377 dataset, thus highlighting differences between these two methods. According to SHAP and LIME, KLRD1 and HCP5 were the most impactful genes.
Cryoanalgesia as the Essential Element of Enhanced Recovery after Surgery (ERAS) in Children Undergoing Thoracic Surgery—Scoping Review
Authors: Zacha Sławomir , Biernawska Jowita
Published: April 2024

This article aims to present cryoanalgesia as an inventive strategy for pain alleviation among pediatric patients. It underlines the tremendous need to align pain management with the principles of the enhanced recovery after surgery (ERAS) approach. The aim of the study was to review the patient outcomes of nerve cryoanalgesia during surgery reported with regard to ERAS in the literature. The literature search was performed using PubMed and Embase to identify articles on the use of cryoanalgesia in children. It excluded editorials, reviews, meta-analyses, and non-English articles. The analysis focused on the study methods, data analysis, patient selection, and patient follow-up. This review includes a total of 25 articles. Three of the articles report the results of cryoanalgesia implemented in ERAS protocol in children. The research outcome indicates shortened hospital stay, potential reduction in opioid dosage, and significant progress in physical rehabilitation. This paper also describes the first intraoperative utilization of intercostal nerve cryoanalgesia during the Nuss procedure in Poland, highlighting its effectiveness in pain management. Adding the cryoanalgesia procedure to multimodal analgesia protocol may facilitate the implementation of the ERAS protocol in pediatric patients.
The Role of Age Variables in Family Language Policy
Authors: Rose Karen , Armon-Lotem Sharon , Altman Carmit
Published: April 2024

Family language policy (FLP) provides a critical framework to explain the planning of language use in the home. It constitutes a dynamic construct that sheds light on variations in the language acquisition of bilingual children, potentially explaining the shifts that may occur in language dominance and preference. The environment and life experiences are thought to shape FLP, yet little is known about the function of age. This study examines the association of FLP with children’s chronological age and the age they become bilingual. Data were collected via questionnaires from parents and their bilingual children (n = 82) aged 5.08–14.08 (M = 8.98, SD = 3.27) speaking English (heritage language) and Hebrew (societal language). Correlations and logistic regressions indicate a relationship between FLP and dimensions of age. Findings reveal that age may have repercussions for parent language beliefs, patterns of language use within the home, and the adoption of language promotion strategies. Younger children and children with a later age of onset of bilingualism are associated with families who lean towards a pro-heritage language FLP. Considering dimensions of age enhances our understanding of FLP and may offer a greater insight into how languages are supported in the bilingual home.
Volumetric Modification of Transparent Materials with Two-Color Laser Irradiation: Insight from Numerical Modeling
Authors: Zhukov Vladimir P. , Bulgakova Nadezhda M.
Published: April 2024

Traditionally, single-color laser beams are used for material processing and modifications of optical, mechanical, conductive, and thermal properties of different materials. So far, there are a limited number of studies about the dual-wavelength laser irradiation of materials, which, however, indicate a strong enhancement in laser energy coupling to solid targets. Here, a theoretical study is reported that aimed at exploring the volumetric excitation of fused silica with dual-wavelength (800 nm and 400 nm) ultrashort laser pulses focused on the material’s bulk. Numerical simulations are based on Maxwell’s equations, accounting for the generation of conduction electrons, their hydrodynamic motion in the laser field, and trapping into an excitonic state. It is shown that, by properly choosing the energies of the two laser harmonics successively coupling with the material, it is possible to strongly enhance the laser energy absorption as compared to the pulses of a single wavelength with the same total energy. Laser energy absorption strongly depends on the sequence of applied wavelengths, so that the shorter wavelength pre-irradiation can yield a dramatic effect on laser excitation by the following longer-wavelength pulse. The predictions of this study can open a new route for enhancing and controlling the highly localized absorption of laser energy inside transparent materials for optoelectronic and photonic applications.
Probing the Magnetic Ground State of Ba2YIrO6: Impact of Nonmagnetic Dopants and Spin–Orbit Coupling
Authors: Halder Shuvajit , Khan Md Salman , Bert Fabrice , Aich Payel , Meneghini Carlo , Ray Sugata
Published: April 2024

Strong spin–orbit coupling (SOC) in iridates has long been predicted to lead to exotic electronic and magnetic ground states. Ba2YIrO6 (BYIO) has attracted particular attention due to the expectation of a Jeff = 0 state for Ir5+ ions under the jj-coupling scheme. However, controversies surround the actual realization of this state, as finite magnetic moments are consistently observed experimentally. We present a multi-physics study of this system by progressively introducing nonmagnetic Sb5+ ions in place of Ir5+ (Ba2YIr​1−ySb​yO​6, BYISO). Despite similar charge and ionic radii, Sb​5+ doping appears highly inhomogeneous, coexisting with a fraction of nearly pure BYIO regions, as confirmed by X-ray diffraction (XRD). This aligns with observations in related compounds. While inhomogeneity creates uncertainty, the doped majority phases offer valuable insights. It is relevant that the inclusion of even small amounts of Sb​5+ (10–20%) leads to a rise in magnetization. This strengthens our previous suggestion that magnetic Ir ions form dynamic singlets in BYIO, resulting in a near-nonmagnetic background. The observed moment enhancement with nonmagnetic doping supports the breakdown of these singlets. Furthermore, the magnetization steadily increases with an increasing Sb​5+ content, contradicting the anticipated approach towards the Jeff = 0 state with increased SOC due to reduced hopping between Ir​5+ ions. This reinforces the presence of individual Ir​5+ moments. Overall, our findings suggest that Ba​2YIrO​6 might not possess sufficiently strong SOC to be solely described within the jj-coupling picture, paving the way for further investigation.
Study on Characteristics of Failure and Energy Evolution of Different Moisture-Containing Soft Rocks under Cyclic Disturbance Loading
Authors: Cao Xuewen , Tang Xuhui , Chen Lugen , Wang Dong , Jiang Yujing
Published: April 2024

During the coal mining process in soft rock mines with abundant water, the rock mass undergoes cyclic loading and unloading at low frequencies due to factors such as excavation. To investigate the mechanical characteristics and energy evolution laws of different water-containing rock masses under cyclic disturbance loading, a creep dynamic disturbance impact loading system was employed to conduct cyclic disturbance experiments on various water-containing soft rocks (0.00%, 1.74%, 3.48%, 5.21%, 6.95%, and 8.69%). A comparative analysis was conducted on the patterns of input energy density, elastic energy density, dissipated energy density, and damage variables of different water-containing soft rocks during the disturbance process. The results indicate that under the influence of disturbance loading, the peak strength of specimens, except for fully saturated samples, is generally increased to varying degrees. Weakness effects on the elastic modulus were observed in samples with 6.95% water content and saturated samples, while strengthening effects were observed in others. The input energy density of samples is mostly stored in the form of elastic strain energy within the samples, and different water-containing samples adapt to external loads within the first 100 cycles, with almost identical trends in energy indicators. Damage variables during the disturbance process were calculated using the maximum strain method, revealing the evolution of damage in the samples. From an energy evolution perspective, these experimental results elucidate the fatigue damage characteristics of water-containing rock masses under the influence of disturbance loading.
On the Lanthanide Effect on Functional Properties of 0.94Na0.5Bi0.5TiO3-0.06BaTiO3 Ceramic
Authors: Zidani Jacem , Alaoui Ilham Hamdi , Zannen Moneim , Birks Eriks , Chchiyai Zakaria , Majdoub Mustapha , Manoun Bouchaib , El Marssi Mimoun , Lahmar Abdelilah
Published: April 2024

The beneficial effects of lanthanide incorporation into 0.94Na0.5Bi0.5TiO3-0.06BaTiO3 (BNT-BT) matrix on its functional properties were investigated. The conventional solid-state method was used for synthesizing samples. The structural refinement revealed that all samples crystallized in R3c rhombohedral symmetry. Raman spectroscopy study was carried out using green laser excitation and revealed that no clear perceptible variation in frequency is observed. Dielectric measurements unveiled that the introduction of rare earth obstructed the depolarization temperature promoted in BNT-BT, the diffusive phase transition decreasing with increasing lanthanide size. Only dysprosium addition showed comparable diffusion constant and dielectric behavior as the unmodified composition. Further, the comparison of the obtained ferroelectric hysteresis and strain-electric field loops revealed that only Dy-phase exhibited interesting properties comparing parent composition. In addition, the incorporation of lanthanides Ln3+ into the BNT-BT matrix led to the development of luminescence characteristics in the visible and near infrared regions, depending on the excitation wavelengths. The simultaneous occurrence of photoluminescence and ferroelectric/piezoelectric properties opens up possibilities for BNT-BT-Ln to exhibit multifunctionality in a wide range of applications.
Analysis of Grindability and Surface Integrity in Creep-Feed Grinding of High-Strength Steels
Authors: Yin Youkang , Chen Ming
Published: April 2024

Creep-feed grinding of high-strength steel is prone to excessive wheel wear and thermal damage defects, which seriously affects the service performance of parts. To solve the above-mentioned issue, a creep-feed grinding test was carried out on high-strength steel using SG and CBN abrasive wheels. The grindability of high-strength steel was scrutinized in terms of grinding force, machining temperature and grinding specific energy. Moreover, the effects of operation parameters and grinder performances on the surface integrity of the workpiece such as surface morphology, roughness, residual stress and hardness were rigorously studied. The results indicate that, when the instantaneous high temperature in the grinding area reaches above the phase transition temperature of the steel, the local organization of the surface layer changes, leading to thermal damage defects in the components. The outstanding hardness and thermal conductivity of CBN abrasives are more productive in suppressing grinding burns than the high self-sharpening properties of SG grits and a more favorable machining response is achieved. The effects of thermal damage on the surface integrity of high-strength steel grinding are mainly in the form of oxidative discoloration, coating texture, hardness reduction and residual tensile stresses. Within the parameter range of this experiment, CBN grinding wheel reduces grinding specific energy by about 33% compared to SG grinding wheel and can control surface roughness below 0.8 µm. The weight of oxygen element in the burn-out workpiece accounts for 21%, and the thickness of the metamorphic layer is about 40 µm. The essential means of achieving burn-free grinding of high-strength steels is to reduce heat generation and enhance heat evacuation. The results obtained can provide technical guidance for high-quality processing of high-strength steel and precision manufacturing of high-end components.
Structural Insights into the Marine Alkaloid Discorhabdin G as a Scaffold towards New Acetylcholinesterase Inhibitors
Authors: Defant Andrea , Carloni Giacomo , Innocenti Nicole , Trobec Tomaž , Frangež Robert , Sepčić Kristina , Mancini Ines
Published: April 2024

In this study, Antarctic Latrunculia sponge-derived discorhabdin G was considered a hit for developing potential lead compounds acting as cholinesterase inhibitors. The hypothesis on the pharmacophore moiety suggested through molecular docking allowed us to simplify the structure of the metabolite. ADME prediction and drug-likeness consideration provided valuable support in selecting 5-methyl-2H-benzo[h]imidazo[1,5,4-de]quinoxalin-7(3H)-one as a candidate molecule. It was synthesized in a four-step sequence starting from 2,3-dichloronaphthalene-1,4-dione and evaluated as an inhibitor of electric eel acetylcholinesterase (eeAChE), human recombinant AChE (hAChE), and horse serum butyrylcholinesterase (BChE), together with other analogs obtained by the same synthesis. The candidate molecule showed a slightly lower inhibitory potential against eeAChE but better inhibitory activity against hAChE than discorhabdin G, with a higher selectivity for AChEs than for BChE. It acted as a reversible competitive inhibitor, as previously observed for the natural alkaloid. The findings from the in vitro assay were relatively consistent with the data available from the AutoDock Vina and Protein-Ligand ANTSystem (PLANTS) calculations.
Comparison of Dimensional Accuracy between a Laser Scanner and a Laser Tracker with Handheld Scan in a Laboratory Setting
Authors: Krummenauer Alex , Bergamo Douglas , Soares Roberto Serpa , Gomes Victor Emmanuel de Oliveira , Nardelli Vitor Camargo
Published: April 2024

The dimensional accuracy of a laser scanner has been extensively evaluated using various measurement methods and diverse reference standards. This study specifically focuses on two key considerations. Firstly, it assesses the dimensional accuracy of the laser scanner by employing another laser scanner, a handheld scanner, as the reference measurement method. Secondly, the study involves the use of three spheres fixed on each wall in both coplanar and non-coplanar positions within a laboratory room at SENAI ISI-SIM. The primary objective is to determine the dimensional accuracy between the centers of the coplanar and non-coplanar spheres up to 10 m. The comparison includes measurement uncertainties, as per ISO GUM standards, obtained using the laser scanner in a laboratory setting with controlled temperature and humidity. Analyzing non-coplanar dimensional accuracy enhances our understanding of the metrological performance of the laser scanner, particularly when assessing the dimensions of objects positioned randomly within a scanning scene.
The Effect of Diagenetic Minerals on the Petrophysical Properties of Sandstone Reservoir: A Case Study of the Upper Shallow Marine Sandstones in the Central Bredasdorp Basin, Offshore South Africa
Authors: Magoba Moses , Opuwari Mimonitu , Liu Kuiwu
Published: April 2024

The upper shallow marine sandstone reservoirs of the Barremian-to-Valanginian formation are the most porous and permeable sandstone reservoirs in the Bredasdorp Basin and an important target for oil and gas exploration. There is a paucity of information on the reservoir characterization and effect of diagenetic mineral studies focusing on the upper shallow marine sandstone reservoirs in the central Bredasdorp Basin; thus, there is a need to investigate the effect of diagenetic minerals and to characterize these reservoirs due to their high porosity and permeability. Datasets, including a suite of geophysical wireline logs, routine core analysis, geological well completion reports, description reports, and core samples, were utilized. A total of 642 core porosity measures, core water saturation, and core permeability data were used for calibration with the log-derived parameters, ranging in depth from 3615 m to 4259 m. Rock samples were prepared for diagenetic mineral analyses, such as thin sections and Scanning electron microscopy, for each well to investigate the presence of diagenetic minerals in the selected reservoir units. The petrophysical analyses showed the results of porosity, volume of clay, water saturation, and permeability, ranging from 9% to 27%, 8.6% to 19.8%, 18.9% to 30.4%, and 0.096 mD to 151.8 mD, respectively, indicating a poor-to-good reservoir quality. Mineralogical analyses revealed that micrite calcite, quartz cement, quartz overgrowth, and authigenic pore-filling and grain-coating clay minerals (illite–smectite and illite) negatively affected intergranular porosity. Porosity-versus-permeability cross plot showed good correlation of 0.86 for ZN1 and 0.83 for ZN3 reservoirs, suggesting that although porosity is the main drive of permeability, there were other geological factors at play, such as diagenetic minerals and compaction.
Towards a Long-Term Unmanned Aerial Vehicle (UAV) Monitoring Framework for Post-Mining Effects: Prosper-Haniel Case
Authors: Pawlik Marcin , Haske Benjamin , Flores Hernan , Bernsdorf Bodo , Rudolph Tobias
Published: April 2024

Direct and indirect effects after mine operations cease operating must ideally be subjected to perpetual monitoring routines in order to detect possible risks or avoid adverse effects on the surrounding ecosystems at an early stage. In this contribution, mining subsidence lakes created inside the nature reserve Kirchheller Heide and Hilsfeld Forest are subjected to analysis for a long-term monitoring scheme. For this purpose, we employ high-resolution unmanned aerial system (UAS)-based multispectral and thermal mapping tools to provide a fast, non-invasive and multitemporal environmental monitoring method. Specifically, we propose to monitor vegetation evolution through multispectral analysis, biotypes identification using machine learning algorithms, and water surface extent detection, together with their thermal behavior. The aim of this contribution is to present the proposed workflow and first results to establish a baseline for future analyses and subsequent surveys for long-term multi-temporal monitoring.