bluuebunny/crossref_metadata_2025 huggingface dataset:
Preconditioning with Melatonin Enhances Osteogenic Differentiation of Dental Pulp Mesenchymal Stem Cells by Regulating MAPK Pathways and Promotes the Efficiency of Bone Regeneration in Calvarial Bone Defects
Authors: Chan Ya-Hui , Ho Kuo-Ning , Lee Yu-Chieh , Chou Meng-Jung , Lew Wei-Zhen , Huang Haw-Ming , Lai Pin-Chuang , Feng Sheng-Wei
Published: September 2021

Background:Mesenchymal stem cell (MSC)-based tissue engineering plays a major role in regenerative medicine. However, the efficiency of MSC transplantation and survival of engrafted stem cells remain challenging. Melatonin can regulate MSC biology. However, its function in the osteogenic differentiation of dental pulp-derived MSCs (DPSCs) remains unclear. We investigated the effects and mechanisms of melatonin preconditioning on the osteogenic differentiation and bone regeneration capacities of DPSCs.Methods:The biological effects and signaling mechanisms of melatonin with different concentrations on DPSCs were evaluated using a proliferation assay, the quantitative alkaline phosphatase (ALP) activity, Alizarin red staining, a real-time polymerase chain reaction, and a western blot in vitro cell culture model. The in vivo bone regeneration capacities were assessed among empty control, MBCP, MBCP+DPSCs, and MBCP+DPSCs+melatonin preconditioning in four-created calvarial bone defects by using micro–computed tomographic, histological, histomorphometric, and immunofluorescence analyses after 4 and 8 weeks of healing.Results:In vitro experiments revealed that melatonin (1, 10, and 100 μM) significantly and concentration-dependently promoted proliferation, surface marker expression (CD 146), ALP activity and extracellular calcium deposition, and osteogenic gene expression of DPSCs (p < 0.05). Melatonin activated the phosphorylation of RUNX-2 and OCN and inhibited COX-2/NF-κB phosphorylation. Furthermore, the phosphorylation of mitogen-activated protein kinase (MAPK) P38/ERK signaling was significantly increased in DPSCs treated with 100 μM melatonin, and their inhibitors significantly decreased osteogenic differentiation. In vivo experiments demonstrated that bone defects implanted with MBCP bone-grafting materials and melatonin-preconditioned melatonin exhibited significantly greater bone volume fraction, trabecular bone structural modeling, new bone formation, and osteogenesis-related protein expression than the other three groups at 4 and 8 weeks postoperatively (p < 0.05).Conclusions:These results suggest that melatonin promotes the proliferation and osteogenic differentiation of DPSCs by regulating COX-2/NF-κB and p38/ERK MAPK signaling pathways. Preconditioning DPSCs with melatonin before transplantation can efficiently enhance MSC function and regenerative capacities.
Risk score model of autophagy-related genes in osteosarcoma
Authors: Qin Wentao , Jiang Mingyang , Hu Yang , Xie Mingjing , Jike Yiji , Bo Zhandong
Published: September 2021

Background

Osteosarcoma (OS) is the most common primary malignancy in children and adolescents, with a high mortality and disability rate. Autophagy plays an important role in the regulation of apoptosis, invasion and metastasis of tumor cells. Hence, construction of a risk score model of autophagy related genes (ARGs) of OS would benefit the treatment and prognosis evaluation.
Methods

We downloaded a dataset of OS from The Cancer Genome Atlas (TCGA) database, and found the OS-related ARGs through Human Autophagy Database (HADb). Five hub ARGs (CCL2, AMBRA1, VEGFA, MYC and EGFR) were obtained by using multivariate Cox regression model. Then we calculated the risk scores and constructed a prediction model. Another two datasets downloaded from GEO were combined to verify the accuracy and validity of the model. The role of immune cell infiltration was systematically explored, and prediction of response to targeted drugs was assessed. Immunohistochemistry was carried out to verify the expression of the key ARGs.
Results

Based on these five hub ARGs, we constructed a risk score model related to OS. High accuracy and validity were demonstrated by datasets downloaded from GEO. These five ARGs played a role in cancer-related biological processes, such as MAPK pathway and PI3K pathway. The results of targeted drug sensitivity analyses coincided with the pathway analysis. Immunohistochemistry showed that the expression of 5 ARGs in OS group was more obvious than that in paracancerous group.
Conclusion

This study constructs a risk score model related to autophagy of OS, explores the prognostic value of autophagy related genes, and finds possible therapeutic targets.
The effect of the hypertensive phase on the long-term outcomes of Ahmed glaucoma valve (AGV) implantation
Authors: Nilforushan Naveed , Hassanpour Kiana , Vafaei Fatemeh , Yadgari Maryam
Published: September 2021

BackgroundTo investigate the long-term effect of hypertensive phase(HP) on the clinical outcomes of Ahmed Glaucoma Valve (AGV) implantation.MethodThe records of patients with different etiologies of glaucoma who underwent AGV implantation were retrospectively reviewed. HP was defined as the IOP> 21 mm Hg during the first three months after surgery. The main outcome measure was cumulative success defined as 5 < IOP< 21 mmHg and 20 % reduction from the baseline with or without IOP lowering medications. The secondary outcome measures were intraocular pressure (IOP) and the number of glaucoma medications. ResultsA total of 120 patients (28 patients of HP, 92 patients without HP) with an average age (±SD) of 48.9 ± 19.6 years and a mean follow-up of 4.5 ± 1.4 years were enrolled. The mean duration of survival was 5.3 ± 0.5 years in HP which was significantly shorter than 6.4 ± 0.2 years in non-HP. (log Rank= 4.2, P= 0.04) Mean IOP and number of IOP lowering agents were higher in postoperative visits at 1,2, 3, and 4 years in HP patients compared with non-HP. (All Ps < 0.01) Higher Baseline IOP was significantly associated with higher rates of surgical failure. Neovascular glaucoma was the only factor associated with the occurrence of HP in a logistic regression model. (Odds ratio: 3.14 CI: 1.2-8.1). Conclusion In the long-term follow up the duration of survival was significantly longer in the non-HP group. In the non-HP group, the success rate was significantly higher than the HP group
Novel Implementation of Multiple Mathematical Models in Automated Storage and Retrieval Warehouse Systems by Using Optimization Algorithms
Authors: Attari Mahdi Yousefi Nejad , Ala Ali , Jami Ensiyeh Neyshabouri
Published: September 2021

Warehouses and distribution centers are among the essential components in the supply chain, and their management is crucial. In the classifications made in the literature, warehouse management methods and distribution centers are divided into manual and machine forms. This system has advantages over previous systems, such as increased flexibility, accuracy, and order preparation speed. In this project, this storage system is called an automatic reverse storage system (ARWS). In this research, a complete and comprehensive explanation about the automatic reverse storage system and its components and their relationship with each other is provided. The following are mathematical models related to ordering items, assigning items to shelves, and completing orders and routing. For validation, the proposed models for small-size problems have been carefully solved with GAMS software. Then, to select the appropriate metaheuristic method, the landscape analysis method has been used. Hence using the analysis method, population-based methods were selected. Taguchi is used to determining the parameters of this method, and its results are presented.
Association of Immediate Reinsertion of New Catheters With Subsequent Mortality Among Patients With Suspected Catheter Infection: a Cohort Study
Authors: Zhong Yiyue , Deng Liehua , Zhou Limin , Liao Shaoling , Yue Liqun , Wen Shi Wu , Xie Rihua , Lu Yuezhen , Zhang Liangqing , Tang Jing , Wu Jiayuan
Published: September 2021

The authors have requested that this preprint be removed from Research Square.
A Novel Waterborne Acrylic Coating Incorporation TIO2 Nanoparticles Modified With Silane Coupling Agent and Ag/zn Zeolite: Mechanical Properties, Thermal Stability, Artificial Weathering Durability and Antibacterial Activity
Authors: Dao Hung Phi , Nguyen Dung Tien , Nguyen Chinh Thuy , Nguyen Hiep Anh , Mac Phuc Van , Tran Trung Huu , Phung Lan Thi , Vu Trung Quoc , Vu Hieu Dinh , Ngo Quyen Thi Cam , Vu Cuong Manh , Nguyen Giang Vu , Tran Lam Dai , Thai Hoang
Published: September 2021

Effect of rutile TiO2 nanoparticles modified with 3-(trimethoxysilyl) propyl methacrylate silane - TMSPM (m-TiO2) and Ag/Zn zeolite on the properties, and durability of acrylic waterborne coatings was studied. The obtained results indicated that m-TiO2 nanoparticles and Ag/Zn zeolite could disperse regularly in the acrylic polymer matrix. Consequently, the abrasion resistance of the acrylic coating was improved in the presence of m-TiO2 or/and Ag/Zn zeolite. The abrasion resistance of the acrylic coating depended on the content of m-TiO2 nanoparticles as well as the initial TMSPM content for modification of rutile TiO2 nanoparticles. The abrasion resistance of the acrylic coating filled by m-TiO2 was higher than that of the acrylic filled by unmodified TiO2 nanoparticles. The acrylic coating filled by 2 wt.% TiO2 modified with 3 wt.% TMSPM had the highest abrasion resistance value, leveling off at 187.16 L/mil. The abrasion resistance of acrylic coating reduced with increasing too high initial TMSPM content for TiO2 modification and m-TiO2 nanoparticles content. The starting temperature of weight loss of acrylic coating filled by m-TiO2 was lower than that of the neat acrylic coating. In comparison with the m-TiO2 nanoparticles, the Ag/Zn zeolite particles showed a lower improvement for acrylic coating properties, i.e. abrasion resistance, weathering durability, but the Ag/Zn zeolite particles expressed an excellent antibacterial activity. Hence, combination of the m-TiO2 nanoparticles and Ag/Zn zeolite particles could enhance the properties of the acrylic waterborne coatings. The acrylic coating filled by 2 wt.% m-TiO2 nanoparticles and 1 wt.% Ag/Zn zeolite particles illustrated high abrasion resistance, good weather durability, and superior antibacterial activity for both of negative gram bacteria (E. Coli) and positive gram bacteria (S. Aureus). This coating is promising for environmental friendly building materials application.
Evaluation of Antimicrobial, Structural, Magnetic, and Thermal Potential of Silver Nanoparticles Anchored Polyaniline-Itaconic Acid-CuO Nanocomposites Synthesized From In-Situ Polymerization Method
Authors: E Parthiban , S Sudarsan
Published: September 2021

The silver nanoparticles (AgNPs) have been embedded within the itaconic acid (IA), polyaniline (PANi), and copper oxide (CuO) to form Ag@PANi-IA-CuO polymeric nanocomposites. In-situ polymerization of itaconic acid has been carried out in the presence of aniline monomers using anhydrous iron trichloride as an oxidising agent. The piper betel leaf extract was used to a reduction of AgNO3. The anchoring of AgNPs onto nanocomposite has been characterized using different techniques such as Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED) X-ray diffraction (XRD), and energy-dispersive X-ray (EDX). Biological, magnetic, and thermal properties of nanocomposites have also been studied in antimicrobial, vibrating sample magnetometer (VSM), and thermogravimetric analysis (TGA). Hence, these types of silver nanoparticles anchored polyaniline-itaconic acid-CuO nanocomposite has shown an attractive application in the field of biomedical and wastewater treatment.
Multi sensor data and temporal image fusion cross validation technique for Agri yield monitoring system
Authors: Shinde Swati V. , Shastri Rajveer , Dwivedi Atul Kumar , Haldorai Anandakumar , Sahni Varsha , Adusumalli Balaji
Published: September 2021

In recent years, the diverse application in various disciplines and the versatility has gained a huge interest for the researchers to research on the multi-sensor data fusion technology. The remote sensing process involves the measurement and recording of the data from a scene. Thus, the remote sensing systems are known to be a powerful tool as they help in the earth's atmosphere and surface monitor at different scales. The remote sensing of the data faces a serious challenge as the data captured by the multiple sensors are heterogeneous. This affects the efficient processing and the effectiveness of the data that is being sensed. Thus, the increase in the diversity in data increases the ancillary datasets. These multimodal datasets are used jointly to improve the processing performance as per the application requirement. Initially, the fusion of the temporal data with the backscattered/temporal data is possible from the data retrieved from remote sensing. Many researchers made several types of research on fusing the multi-temporal and multimodal data and gave different ideas for a different type of researchers. This paper presents the cross-validation technique for monitoring the yield. This monitoring system is developed by fusing the multi-sensor data and the temporal images. This fusion is performed, and the performance of the yield monitoring system is analyzed from the results obtained. By using the cross-validation technique, the efficiency of the system is found to be improved.
Triptolide exhibits anti-inflammatory effects on adipocytes and macrophages by inhibition of AMPK/mTOR pathway
Authors: Li Xue-li , Liu Zhao
Published: September 2021

Obesity is a growing global health problem and chronic over-nutrition disease with lipid accumulation that results in low-grade chronic inflammation in the microenvironment of adipose tissue. Triptolide is a diterpene lactone compound extracted from the roots of the Chinese herb TWHF and possesses a therapeutic potential due to its immunosuppressive and anti-inflammatory properties. In this study, we built obesity-related inflammatory models of adipocytes using LPS, Ma-CM and raw264.7 macrophages, while the obesity-related inflammatory models of macrophages were built using LPS and Ad-CM system. We used these inflammatory models to investigate the anti-inflammatory property of triptolide. Treatment of triptolide (0.005, 0.010, 0.020 and 0.040 μ M) inhibited LPS-induced or macrophages conditioned medium-stimulated activation of AMPK/mTOR signaling pathway (p < 0.05). The results showed that triptolide reduced the release of chemokines MCP-1, RANTES, EOTAXIN and KC in LPS, Ma-CM or RAW264.7 macrophages-stimulated 3T3-L1 adipocytes. Triptolide also diminished MCP-1, RANTES, EOTAXIN, KC and TNF-α in Ad-CM stimulated RAW264.7 macrophages, while expression of MCP-1, RANTES, TNF-α, GM-CSF and IL-6 was decreased in LPS stimulated RAW264.7 macrophages (p < 0.05). These results demonstrate that triptolide is not only effective against inflammatory response of RAW264.7 macrophages or 3T3-L1 adipocytes, but also disrupts the crosstalk between macrophages and adipocytes, particularly by inhibiting secretion of pro-inflammatory mediators through inhibiting the activation of AMPK/mTOR signaling pathway. Triptolide might benefit to ameliorate obesity-induced inflammatory diseases.
Preoperative bacterial culture can predict severe pneumonia in patients receiving esophagectomy
Authors: Kaneta Akinao , Sato Takahiro , Nakano Hiroshi , Matsumoto Takuro , Tada Takeshi , Watanabe Yohei , Hanayama Hiroyuki , Hayase Suguru , Saze Zenichiro , Kono Koji
Published: September 2021

Background

Postoperative pneumonia is one of the major complications after esophagectomy. The aim of this study was to determine whether bacterial cultures before esophagectomy could predict occurrence of postoperative pneumonia and help treatment strategies for postoperative pneumonia.
Methods

Sixty-nine patients who underwent subtotal esophagectomy at Fukushima Medical University hospital between January 2017 and May 2021 were included in this study. We collected sputum, oral and/or nasopharyngeal swabs for bacterial culture preoperatively from all patients and from those who were suspected of postoperative pulmonary infections. We compared cultured pathogenic bacteria obtained preoperatively and postoperatively from patients who developed postoperative pneumonia, and investigated their association with incidence of postoperative pneumonia. Fisher’s exact test was used to compare categorical variables between groups, and Wilcoxon test was used to compare continuous variables. Risk factors for postoperative pneumonia and severe pneumonia were analyzed using multivariate logistic regression models.
Results

Postoperative pneumonia occurred in 22 (31%) of the 69 patients, and 13 of the 22 patients were classified as with severe pneumonia. Multivariate analysis revealed that longer operative duration (for 30 minutes increase; odds ratio 1.27, 95% confidence interval 1.01–1.51, p=0.039) and positivity for preoperative bacterial culture (odds ratio 5.03, 95% confidence interval 1.31–19.2, p=0.018) were independent risk factors for severe postoperative pneumonia, but not for all incidence of postoperative pneumonia. Of note, in only 5 out of the 22 patients with pneumonia, the same pathogen species were detected preoperatively and after the onset of pneumonia.
Conclusions

In conclusion, our results implied that preoperative bacterial culture may be useful to predict severe postoperative pneumonia. However, it may not be useful in determining pathogenic bacteria responsible for postoperative pneumonia.
Red-Emitting Phosphor Ca2MgSi2O7: Sm3+, Eu3+ for UV White Light-Emitting Diodes
Authors: Tuerxun Ayinuremu , Jilili Subiyinuer , Wang Lei , Sidike Aierken
Published: September 2021

In this research, a series of Ca2MgSi2O7: 2% Sm3+, x% Eu3+ (x=4, 5, 6, 7, 8) red phosphors were synthesized using the high-temperature solid-phase method. The phase and luminescence properties of the samples were investigated using an X-ray diffractometer and a photoluminescence spectrometer. The results showed that the synthesized samples were of pure phase, and the introduction of small amounts of Sm3+ and Eu3+ had no significant impact on the crystal structure of the phosphors. It is observed from the phosphor spectra that the co-doped samples exhibited intense red-light emission corresponding to that of Eu3+ at 613 nm when excited at the strongest excitation peak of Sm3+ (401 nm); here, the emission peak intensity of Eu3+ increased by a factor of 5.3. It is found that resonant nonradiative energy transfer occurs from Sm3+ to Eu3+ in the sample, and the energy transfer efficiency reaches up to 44%. The calculated critical distance for energy transfer is 16.117 Å and the concentration interrupt mechanism is an electric dipole-dipole (d-d) interaction. The color coordinates of the samples are all located in the red region (0.6319, 0.3676) with a color purity of ~ 89.3%. The samples exhibited thermal stability of up to 68.6% of that at room temperature when heated to 150˚C . The LED samples packaged with the phosphors emitted warm white-light with a color temperature (CCT) of 5553 K and a color rendering index (Ra) of 84.8. The magnesium yellow feldspar silicate phosphor is suitable as the red component in white LED trichromatic phosphors and has potential applications in solid-state lighting.
Increasing aridity may reduce productivity and soil organic carbon storage with woody plant encroachment
Authors: Liu Yunhua , Cheng Junhui , Schmid Bernhard , Sheng Jiandong
Published: September 2021

Aims

We have found a positive effect of woody plants on total plant carbon (C) storage in less arid grassland was shifted to a negative effect in arid grasslands in Xinjiang, a typical arid region in China. In this study, we further assessed the effects of woody plants on aboveground primary productivity (ANPP) and soil organic C (SOC) storage and explored the mediation of climate conditions on these effects. We also aimed to elucidate the reasons for the effects on SOC storage in terms of ANPP and belowground biomass C (BGC).
Methods

We compared the difference in ANPP and SOC content between pure and wooded grasslands and evaluated the relation between SOC content and ANPP and BGC in six grassland types along the altitude (climatic) gradients.
Results

In three arid types, woody plants had a negative effect on ANPP due to their more negative impacts on herbaceous plants and lower ANPP. The negative effect on ANPP and BGC led to that on SOC storage in these types. In less arid types, there had a positive effect on ANPP because woody plants had weaker negative impacts on herbaceous plants and higher ANPP. A positive effect on ANPP combined with a neutral impact on BGC contributed to a positive effect on SOC storage in these types.
Conclusions

Woody plants had a negative effect on ANPP and SOC storage in most arid grasslands in Xinjiang. We predicted that increasing aridity may reduce ANPP and SOC storage with woody plant encroachment in the future.
Crystalloids vs. colloids for fluid optimization in patients undergoing brain surgery
Authors: Markovic-Bozic Jasmina , Music Polona , Visocnik Bozidar , Spindler-Vesel Alenka
Published: September 2021

BACKGROUND This randomised, double-blinded, single-centre study prospectively investigated the impact of goal directed therapy and fluid optimization with crystalloids or colloids on perioperative complications in patients undergoing brain surgery. METHODS 80 patients were allocated into two equal groups to be optimised with either crystalloids (n=40) or colloids (n=40). Invasive hemodynamic monitoring and optimization with fluids and vasoactive drugs were used to adjust and maintain mean arterial pressure and cerebral oxygenation within the baseline values (± 20%) and stroke volume variation (SVV) ≤13. Postoperative complications from different organ systems were monitored during the first 15 days after surgery. Hospital stay and mortality were also recorded. RESULTS Crystalloid group received significantly more fluids (p = 0.003) and phenylephrine (p = 0.02) compared to colloid group. This did not have any significant impact on intraoperative or postoperative complications, hospital stay or mortality, where no differences between groups were observed. CONCLUSIONS Either crystalloids or colloids could be used for fluid optimization in brain surgery. If protocolised perioperative haemodynamic management is used, the type of fluid does not have significant impact on the outcome. CLINICAL TRIAL REGISTRATION: Identified as NCT03249298 at www.clinicaltrials.gov KEY WORDS: Brain surgery, fluid optimization, haemodynamic management
Evolution of acoustic communication in fungus-growing ant societies
Authors: Murakami Takahiro , Sakamoto Hironori , Higashi Seigo
Published: September 2021

Do ants ‘talk’? If so, how important is talking in ant societies? Chemical communication, through pheromones for example, was thought to be the main communication tool in ant societies; however, a major question is whether chemical substances alone can control highly differentiated social behaviours. In this study, we focused on the ‘talk’ of agricultural ants, key organisms in the Neotropical ecosystem, and conducted a detailed acoustic analysis. Our results indicate that in fungus-growing ants, acoustic communication is a much more frequent and critical factor than previously believed. The frequency of stridulatory sound-production recordings from the ants were found to be significantly correlated with social structure complexity. Structural analysis indicated that both the area and number of slits in the stridulatory acoustic organs were significantly correlated with body size. The ability of leaf-cutting ants to maintain fungus gardens was significantly lower in the sound-inhibited group than in the pheromone secretion-inhibited group. These results suggest that ants that have become ‘chattier’ may induce altruistic behaviours and create more complex societies. The findings of this study may be applicable not only to social evolution studies but also for effectively controlling ant behaviours.
The mechanical force of minority cells modulates the tumor taxol-resistance and progression
Authors: Zhang Di , Feng Xueyan , Wang Guoxun , Lu Liwei , Feng Feng , Wang Xiuyu , Yu Chanchan , Chai Yahong , Ge Maofa , Yao Li
Published: September 2021

Cancer is a leading cause of death worldwide. The chemotherapy is one of a major treatment for cancer. However, resistance to chemotherapeutic agents is still a crucial problem in cancer therapies. Majority drug resistance results from the accumulation of mutation of genes in the minority-resistant cells. The mechanism underlying the emergence and the development of cancer resistance from minority-resistant cells has not been fully elucidated. Here, we revealed that minority taxol-resistant cancer cells (MRCs) with enhanced mechanical force can transmit the high force to surrounding sensitive cells through force transducer merlin, and thus enhancing the contraction and adhesion strength of tumor cells (termed as mechanoassimilation), which eventually accelerates the development of drug resistance and tumor progression in vivo. In addition, disturbance and reduction of mechanoassimilation in tumor leads them sensitive to taxol again in vitro and in vivo, which also provide a preliminary indication of MRC contribution in drug-resistance and malignancy development through mechanoassimilation, and offer a new opportunity for cancer therapy by targeting the tumor mechanics.
Development of anti-Jk3 associated with silenced Kidd antigen expression and a novel single nucleotide variant of the JK gene
Authors: Manrai P.A. , Siddon A.J. , Hager K.M. , Hendrickson J.E. , Keller M.A. , Tormey C.A.
Published: September 2021

Anti-Jk3 is a rare alloantibody to a high-prevalence antigen primarily seen in individuals of Polynesian descent and is associated with a handful of well-established variants of the SLC14A1 gene. We report a case of the Jknull phenotype, associated with formation of anti-Jk3, in a patient of non-Polynesian descent. This patient, a 51-year-old woman self-described as of Jamaican and Scottish ancestry, presented to our hospital for oncologic care. The patient’s blood sample typed as blood group A, D+. All screening and panel reagent red blood cells showed reactivity, ranging from 2 to 4+; autocontrol and direct antiglobulin test were both negative. Antigen phenotyping revealed Jk(a–b–), leading to suspicion for anti-Jk3, which was subsequently confirmed by our immunohematology reference laboratory. Given her reported familial background, testing of the SLC14A1 gene was performed, revealing that the patient was heterozygous for the single nucleotide variant (SNV) at c.838G>A in exon 8 and therefore carries both JK*01 and JK*02 alleles that encode Jka and Jkb, respectively. However, the patient was found to be heterozygous for several additional SNVs: c.28G>A in exon 3; c.191G>A, c.226G>A, and c.303G>A in exon 4; and c.757T>C in exon 7. The patient’s Jk(b–) phenotype can be explained by coinheritance of c.838A with c.191G>A, which defines null allele JK*02N.09. Coinheritance of SNVs c.28G>A and c.838G with rare SNV c.757C that is predicted to cause a non-conservative amino acid change (p.S253P) likely accounts for the complete serologic absence of Jka and the ability to form anti-Jk3 in this case. This finding would represent a new JK*01 null allele. This evaluation illustrates the importance of genetic analysis in identifying the factors preventing a high-prevalence antigen from being expressed, particularly when discovered outside of an expected racial or ethnic group.
A mild case of hemolytic disease of the fetus and newborn due to anti-Sc2
Authors: Núñez Ahumada M.A. , Arancibia Aros C.E. , Villalobos Pavez C.E. , Pontigo Gonzalez F.M. , Abarca Arce V. , Sandoval Medrano M. , Reyes Jorquera S.
Published: September 2021

We report the case of a newborn girl with jaundice due to increased indirect bilirubin with a positive direct antiglobulin test (DAT) and compensated hemolysis. The result of the newborn’s DAT was discrepant with the negative result of the mother’s indirect antiglobulin test. The multiparous mother had a previous history of fetal hydrops miscarriage, with no known cause, and no record of the cause was found at the hospital where she was treated. After referring samples from the mother and newborn to a reference laboratory, the rare alloanti-Sc2 was identified in the mother’s plasma and in the newborn’s eluate. HEA BeadChip genotyping of the newborn’s DNA sample predicted the SC:1,2 phenotype.
A unique approach to screen for blood donors lacking high-prevalence antigen Inb of the Indian blood group system
Authors: Joshi S.R. , Senjaliya S.B. , Maru H.D. , Kshirsagar P.D. , Kulkarni S.S. , Shrivastava P.
Published: September 2021

The Inb antigen of the Indian blood group system is a high-prevalence antigen. The presence of alloanti-Inb in a recipient may pose a problem in finding compatible blood for transfusion. The aim of this study was to screen blood donors for Inb and to include individuals found to be In(b–) in our rare donor registry. To save resources, a unique study design was constructed. Blood group O donors were tested for Inb because their red blood cell (RBC) units could serve recipients across all ABO groups. EDTA blood samples were used for serologic and genomic testing. These samples were first tested serologically for Ina, and samples typed as In(a+) were then tested both serologically and molecularly for Ina and Inb to find homozygous IN*01/01 [i.e., the predicted In(b–) phenotype]. A cost-conservative approach in using recycling of antibody was adopted to economize available resources. Of 6300 donors, 196 donor samples typed as In(a+) and were also found to be In(b+) when tested by serologic and genomic methods. Although none of the donors typed as In(b–), the statistical analysis suggests the expected prevalence for this rare phenotype to be 0.02 percent among the total number of donors tested. In conclusion, this report presents a unique cost-conservative approach using limited reagents to screen a large number of donors for the rare In(b–) phenotype.
The preferred leadership styles in vocational training corporations: Case of Jordan
Authors: Al-Khasawneh Akif , Eyadat Haitham , Elayan Malek
Published: September 2021

The study aims to identify the leadership styles (democratic, authoritative, autocratic, laissez-faire, transformational, and transactional) practiced by trainers at the Vocational Training Corporation as perceived by the female trainees in Jordan. Descriptive statistics were used to analyze the data collected by the questionnaire from respondents (n = 105 female trainees) selected by the simple randomization sampling and representing 50% of the population (n = 210). Among the questionnaires sent, only 104 were received and declared valid for statistical analysis. Major findings indicated that the democratic leadership style was mostly preferable by female trainees, where rated top by respondents (M = 4.50), followed by the transformational style (M = 4.23), and the authoritative style was rated the lowest (M = 2.58) compared with the autocratic, laissez-faire and transactional leadership styles. Thus, to keep increasing the development of training programs and obtain positive feedback for trainees, both trainers and managers of the Vocational Training Corporation necessarily need to practice appropriate leadership styles, develop a high level of learning and benefits. AcknowledgmentsThe authors would like to thank Al-Balqa Applied University in the Hashemite Kingdom of Jordan and the Institute of Public Administration in Kingdom of Saudi Arabia for all kinds of support and providing us with time and access to the research resources.
Two Woody Scented Oils from the Amazonian Forest
Authors: Mattoso Eduardo , dos Anjos Charlene , Barata Lauro , Marsaioli Anita
Published: September 2021

The essential oils from Aniba parviflora and the balsam from Protium rubrum, which are two woody scented Amazonian species, were analyzed by gas chromatography-mass spectrometry (GC-MS), 1H and 13C nuclear magnetic resonance (NMR). GC-MS analysis revealed the presence of unknown constituents. These compounds were then isolated, analyzed and characterized by NMR. Both substances presented distinct woody notes that contributed to the bulk woody character of the crude oils.
Effect of supplementation with core homeopathic on the serum immunological profile of pregnant ewes and lambs
Authors: Monteiro-Toma Claudia Dias , Toma Hugo Shisei , Muraro Lívia Saab , Oba Eunice , Almeida Jéssica Núbia da Silva , Carvalho Armando de Mattos , Rêgo Fabiola Cristiane de Almeida , Santos Marcelo Diniz dos
Published: September 2021

Few information is available about the optimal period for colostrum absorption in the bowel of newborn lambs. The aim of the current study was to investigate the effects of using supplementation with commercial core homeopathic during pregnancy and early lactation on 18 pregnant Santa Inês ewes presenting body condition score ≥ 2.5. Pregnant animals were randomly divided in two groups; one was used as control (control group) and the other was supplemented on a daily basis with 30 g of homeopathic product with Natrum muriaticum 10-60, Calcium carbonicum 10-30, Silicea terra 10-400 and Hypothalamus 10-30 per animal, mixed to the concentrate during pregnancy and early lactation (treatment group). The Total Protein (TP) and globulin (GLB) values found in ewes and lambs belonging to the treatment group were higher than and different from those recorded for the control group. The serum albumin (ALB) concentration did not differ between groups of ewes but was different in lambs. TP and GLB evolution in lambs and ewes throughout the seven experimental days showed the animals in the treatment group trend to present higher TP and GLB values in all experimental periods; supplemented animals showed trend to progressive increase in both variables until day 3; whereas non-supplemented animals showed such trend until day 2 (when they reached their peak). The commercial core homeopathic to the animals during pregnancy and early lactation resulted in higher TP and GLB concentrations in the serum samples collected from ewes and lambs.
Immunolocalization of angiotensin-(1-7), angiotensin II and angiotensin-converting enzyme 2 in goat ovary
Authors: Feitosa Lauro César Soares , Pereira Alécio Matos , Carvalho Adeline Andrade , Sousa Junior Antônio de , Honorato-Sampaio Kinulpe , Costa Amilton Paulo Raposo
Published: September 2021

There is increasing evidence as to the participation of the ovarian renin-angiotensin system in important reproductive processes. The inhibition of the angiotensin-converting enzyme (ACE) caused an increase in the rate of ovulation and pregnancy in the artificial insemination protocol has fixed time (TFIA). This study aimed to investigate the presence and location of Ang II, Ang- (1-7) and ACE2 in goat ovaries and the possibility of the involvement of these peptides in previous results. Ten ovaries from goats were collected in a slaughterhouse, washed in buffered PBS, perfused with protease inhibitor solution and processed for immunohistochemistry protocol. The search for peptides was performed using the avidin–biotin–peroxidase method. A strong immunoreactivity for Ang II in theca cells of antral follicles and corpus luteum was observed. Antral follicles (theca cells), corpus luteum and oocyte cytoplasm in early antral follicles exhibited strong immunoreactivity for Ang-(1-7). There was strong immunoreactivity for ACE2 in the cytoplasm of luteal cells and theca cells of antral follicles. In this study, for the first time, the presence and location of Ang II, Ang-(1-7) and ACE2 are reported in goat ovary, suggesting that there is participation in follicular development, oocyte maturation and corpus luteum development.
Inverted ovariosalpingohisterectomy technique in cats: advantages and limitations
Authors: Campos Ana Carolina de Souza , Fernandes Maria Eduarda dos Santos Lopes , Lima Virgínia Conceição Tavares , Silva Michelle Lussac , Oliveira William de Almeida , Teixeira Giovanna Finklstein , Ferri Ana Elisa da Cruz , Silva Marta Fernanda Albuquerque da
Published: September 2021

The inverted ovariosalpingohysterectomy (OSH) technique involves initial uterine removal, followed by ovarian disinsertion. The objective of this study was to compare the inverted technique with the traditional technique, characterized by initial removal of the ovaries followed by uterine resection, for evaluating the performance of the inverted OSH technique in cats. For this, 24 healthy, female cats, aged between 7 months and 5 years, with a body condition score of up to 6 points (9-point scale), divided into two equal groups: the traditional technique (TT) and the inverted technique (IT). Procedures were performed by the same veterinarians (surgeon and their assistant) as well as semi-quantitative analysis of the techniques (questionnaire related to the ease of uterine and ovarian manipulation as well as of intestinal and urinary vesicle manipulation) and descriptive qualitative analysis (technical report with the opinion of surgeons and an external evaluator). In addition, the total surgical time and positive pain response were evaluated, indicated by the changes in vital parameters observed by the anesthesiologist during surgery. Inverted technique it promoted greater ovarian exposure, allowing uterine cranial flexion to perform the ligatures, thus facilitating their manipulation, hemostasis, and section of the pedicle. Free the uterus from its ligaments to the abdominal cavity allowed greater mobilization of the conjoint. Both techniques required short operation time (average time of 16’± 1.321’ in the IT group and 15’± 0.525’ in the TT group). The presence of algetic stimulus was similar in the techniques, three animals in each group suffered analgesic rescue.
Tubular subdermal flap to dorsal nasal reconstruction in a dolichocephalic dog: case report
Authors: Mascella Vivian , Gallina Marina Frazatti , Santos Larissa dos , Brilhante Natalia dos Santos , Martin Paulo Roberto , Santos Ivan Felismino Charas
Published: September 2021

This case report described the use of tubular subdermal flap to dorsal nasal reconstruction of an old dolichocephalic dog. A nine-year-old Dachshund female dog-weighing 10 kg presented with deep and extensive wound in the dorsal nasal region was attended at veterinary clinic, Sorocaba city, São Paulo, Brazil. The reconstructive surgical procedure was performed in three stages with an interval time of 25 days, which included the preparation of the tubular subdermal flap; the advance of the flap to the region of the tissue defect, and the removal of excess flap. The region healed 14 days after the last stage. The tubular subdermal flap was effective for treating ulcerative epithelial wounds associated with interstitial lymphoplasmocyte dermatitis and vasculitis of the dorsal nasal region of an old Dachshund dog.
Poly(ADP-Ribose) Polymerase-1 Inhibitors Drug Discovery, Design, and
Development as Anticancer Agents from Past to Present: A Mini-Review
Authors: AlGhamdi Arwa , AlMubayedh Hanine
Published: September 2021

Cancer treatments are known for their life-threatening toxicities attributed to their low
selectivity; hence, new therapeutic approaches are being developed as alternatives. Among those
approaches is the DNA repair mechanism, where its inhibition results selectively in the death of
cancerous cells. Poly(ADP-Ribose) Polymerase (PARP) is one of the enzymes involved in the repair
of damaged DNA. The inhibition of PARP shows to be a promising approach for effective targeted
treatment of cancer, especially in tumours with pre-existing Homologous-Repair (HR) defects (i.e.,
BRCA). Nicotinamide, which is one of the PARP catalytic products, was the first identified PARP
inhibitor (PARPi). The first FDA-approved PARPi was Olaparib in 2014 for the treatment of BRCA
mutated advanced ovarian cancer. Several clinical trials have been conducted to further improve
PARPi. However, there are some concerns related to drug resistance, PARPi sensitive-tumour identification,
and toxic accumulation of PARPi. This report will review the uses of PARPi, drug design
and development of PARPi from past to present, current issues, and prospective plans.
BORDERLINE PERSONALITY DISORDER DURING COVID-19 PANDEMIC
Authors: Yudhantara Dearisa , Istiqomah Ratri
Published: September 2021

The COVID-19 pandemic has had a significant impact on patients diagnosed with Borderline Personality Disorder (BPD). BPD patients who are unable to see a therapist due to limitations during the pandemic may experience feelings of uncertainty, misperceptions, misunderstandings, and even paranoid ideas that are usually associated with stress. In patients who have previously undergone psychotherapy, there may be negative effects during this pandemic. The majority of providers/therapists, on the other hand, stated that their experience exceeded their expectations and that they had a favorable assessment of patient acceptance.
31º Encontros da Imagem: Génesis 2.1
Authors:   Associação Encontros da Imagem
Published: September 2021

GÉNESIS 2:1, foi o tema escolhido para a 31ª edição dos Encontros da Imagem – Festival Internacional de Fotografia e Artes Visuais.2021, que este ano decorre entre 17 de setembro e 31 de outubro. Entre as muitas outras atividades, o Festival engloba 47 exposições distribuídas por 25 espaços distintos, envolvendo a participação de 64 fotógrafos. GÉNESIS 2:1 dá continuidade ao tema do ano passado e, nunca um tema escolhido, se enquadrou tão bem no contexto da atualidade. Um ano depois, voltamos também nós e todo o mundo — em resultado da crise pandémica provocada pelo Covid-19, de novo, a ter que passar por um confinamento generalizado. Gerou-se a confusão e o caos. Uma incapacidade coletiva para compreender a desordem das coisas, confrontando a humanidade com desafios cada vez mais complexos e exigentes. A sociedade contemporânea está desde há muito, perante enormes desafios de carácter global: desde as questões relacionadas com o planeta e os seus problemas ecológicos — perda da biodiversidade, alterações climáticas, aquecimento e contaminação — até às civilizações que o habitam, onde muitas delas geram novas desigualdades e indiferença moral — regimes políticos, religiosos, fronteiras, refugiados, racismo, questões de género e muitas outras. Aquilo a que chamamos progresso, não só deixou de coincidir com a humanização do mundo, como pode acabar por ditar o seu fim. Urge encontrar soluções para acabar com as desigualdades e indiferença em relação ao sofrimento de milhões de pessoas. Uma interrogação se pode colocar: que futuro nos espera? A crise em que vivemos constitui uma oportunidade para que todos encontremos causas comuns e discutamos as melhores soluções para o que deva ser feito. Assim, e também com o objetivo de alerta, muitas das exposições agora apresentadas no âmbito dos Encontros da Imagem, para além do seu lado estético, abordam algumas das questões pertinentes que o mundo contemporâneo hoje vive.
A mulher na legislação educacional paraense na transição do Império para a República
Authors: Reschke Monika , Mazzini João Lúcio , Damasceno Alberto
Published: September 2021

Este artigo analisa a legislação educacional paraense a partir do o último regulamento da instrução pública no Império, Portaria de 29 de abril de 1871, fazendo um paralelo com o Decreto nº 149 de 7 de maio de 1890, primeiro do período republicano. A indagação foi se houve avanços nos direitos femininos relativos à instrução pública nesses documentos legais? Para responder traçamos os objetivos de identificar as principais semelhanças e diferenças nas legislações mencionadas; verificar o processo de inserção da mulher tanto como discente quanto docente no sistema público de instrução e detectar as principais características da educação feminina. Trata-se de uma pesquisa histórica, bibliográfica e documental. Após seleção e análise das fontes com base em Bardin (2010), foi possível inferir que houve uma maior participação das mulheres na instrução a partir do advento da República, inclusive com o direito de voto e candidatura a cargo no Conselho Superior de Instrução Publica.
Iracy Doyle
Authors: Da Silva Marlon Silveira da Silva , Blaudt Vanessa Lima Blaudt , Caetano Marcio Rodrigo Vale
Published: September 2021

Através de um olhar sobre sua biografia (vida e obra), buscaremos narrar alguns aspectos considerados importantes para a compreensão dos estudos desenvolvidos pela psiquiatra carioca Iracy Doyle, além dos/as intelectuais que contribuíram e tiveram influência sobre suas pesquisas, de modo a nos aproximarmos do lugar de onde produzia seus estudos e as condições que possibilitaram a emergência de tais investimentos. Vale destacar que Iracy Doyle foi a primeira mulher médica a escrever, no Brasil, uma tese sobre a homossexualidade feminina, distanciando-se significativamente das demais conclusões de seus pares (médicos homens) a respeito das causas que levariam à inversão sexual das mulheres.
The past, present, prospects and problems of improving the specifi c prevention of brucellosis
Authors: Korshenko V. A. , Shchipeleva I. A. , Kretenchuk O. F. , Markovskaya E. I.
Published: September 2021

Brucellosis remains the most widespread zoonotic infection in the world. Th e spread of the infection is controlled by animals vaccination because the high morbidity rate of the population is associated with the spread of infection among livestock. Th e research is ongoing on a commercial preparation of an eff ective and safe vaccine for immunization of humans and animals against brucellosis. Th e review is devoted to the prospects and problems of improving the specifi c prevention of brucellosis. Th e authors analyzed scientifi c publications from various databases of electronic libraries, such as PubMed, e-library, CyberLeninka, etc.
Modern view of the problem of missed abortion
Authors: Andreeva M. V. , Shevtsova E. P. , Zabolotneva K. O. , Lyutaya E. D. , Sivko T. S.
Published: September 2021

Non-developing pregnancy is an urgent obstetric problem, which is included in the structure of reproductive losses and. The authors performed a systematic analysis of the data presented in the modern scientifi c literature on the epidemiology, etiology, pathogenesis, and diagnosis of non-developing pregnancy. Th e review includes data from foreign and domestic articles on this topic found in PubMed over the past 7 years.