bluuebunny/crossref_metadata_2025 huggingface dataset:
Potential of Fermented Fruit Peel Liquid in Cosmetics as a Skin Care Agent
Authors: Khang Do Tan , Tien Le Thi Thuy , Men Tran Thanh , Thuy Nguyen Phuong
Published: May 2021

The purpose of this study was to evaluate the antioxidant, antibacterial, and anti-UVB of three kinds of fermented fruit peel extracts by pectinase enzyme, including pomegranate, pomelo, and banana peel. The antioxidant was evaluated by the DPPH (2,2-Diphenyl-1-picrylhydrazyl) free radical scavenging method. Antibacterial activity against Escherichia coli and Staphylococcus aureus was assessed by disk diffusion assay, and the photoprotective activity was measured using a photospectrometric method. The results revealed that pomegranate peel extract at 3000 IU/g peel exhibited potent free radical scavenging property against DPPH, with the smallest IC50 value at 0.18%, which was better than that of pomelo peel extract at 55.79%. Furthermore, the results suggested that the pomegranate peel extract also exhibited antibacterial activity against E. coli better than pomelo peel extract, but none of the three samples shows the antibacterial capacity against S. aureus. Moreover, 10% pomegranate peel extract also expressed the strongest anti-UVB activity, with an SPF value of 36.582. The research demonstrates pomegranate peel’s bioactivity potential for further experiments.
Potent Tyrosinase Inhibitory Activity of Curcuminoid Analogues and Inhibition Kinetics Studies
Authors: Athipornchai Anan , Niyomtham Nattisa , Pabuprapap Wachirachai , Ajavakom Vachiraporn , Duca Maria , Azoulay Stéphane , Suksamrarn Apichart
Published: May 2021

Natural tyrosinase inhibitors from herbal plants are promising therapeutic agents for skincare and cosmetic products. Natural curcuminoids exhibit weak antityrosinase properties. The structural modification of curcumin, the major curcuminoid from Curcuma longa, gave 14 analogues. The tyrosinase inhibitory activity of the natural curcuminoids and the modified analogues on both L-tyrosine and DOPA substrates were evaluated. The inhibition kinetics were also undertaken. For analogues with potent activity on the L-tyrosine substrate, the isoxazole analogue 12 and two reduced analogues, hexahydrocurcumin (16) and the α,β-unsaturated analogue 17, showed IC50 values of 8.3, 14.6 and 9.4 µM, and were 20.9-, 11.9- and 18.4-fold more active, respectively, than kojic acid, the reference compound. For the analogues with potent antityrosinase on DOPA substrate, the dimethylated analogue 5 exhibited the strongest antityrosinase activity against the DOPA substrate, with the IC50 value of 8.0 µM, which was 16.6-fold more active than kojic acid. The inhibition kinetics revealed that curcuminoid 5 could bind with both free enzyme and with the enzyme–substrate complex. It acted as a competitive–uncompetitive mixed-II type inhibitor. Curcuminoid 17 could bind with both free enzyme and the enzyme–substrate complex. The results indicated that 17 acted as a competitive–uncompetitive mixed-I type inhibitor, while curcuminoid 12 was a noncompetitive inhibitor which bound with both free enzymes and the enzyme–substrate complex. These potent analogues might serve as new potential tyrosinase inhibitors for the prevention and treatment of skin pigmentation disorders.
UAV-Based Classification of Cercospora Leaf Spot Using RGB Images
Authors: Görlich Florian , Marks Elias , Mahlein Anne-Katrin , König Kathrin , Lottes Philipp , Stachniss Cyrill
Published: May 2021

Plant diseases can impact crop yield. Thus, the detection of plant diseases using sensors that can be mounted on aerial vehicles is in the interest of farmers to support decision-making in integrated pest management and to breeders for selecting tolerant or resistant genotypes. This paper investigated the detection of Cercospora leaf spot (CLS), caused by Cercospora beticola in sugar beet using RGB imagery. We proposed an approach to tackle the CLS detection problem using fully convolutional neural networks, which operate directly on RGB images captured by a UAV. This efficient approach does not require complex multi- or hyper-spectral sensors, but provides reliable results and high sensitivity. We provided a detection pipeline for pixel-wise semantic segmentation of CLS symptoms, healthy vegetation, and background so that our approach can automatically quantify the grade of infestation. We thoroughly evaluated our system using multiple UAV datasets recorded from different sugar beet trial fields. The dataset consisted of a training and a test dataset and originated from different fields. We used it to evaluate our approach under realistic conditions and analyzed its generalization capabilities to unseen environments. The obtained results correlated to visual estimation by human experts significantly. The presented study underlined the potential of high-resolution RGB imaging and convolutional neural networks for plant disease detection under field conditions. The demonstrated procedure is particularly interesting for applications under practical conditions, as no complex and cost-intensive measuring system is required.
Modelling the Material Resistance of Wood—Part 2: Validation and Optimization of the Meyer-Veltrup Model
Authors: Brischke Christian , Alfredsen Gry , Humar Miha , Conti Elena , Cookson Laurie , Emmerich Lukas , Flæte Per Otto , Fortino Stefania , Francis Lesley , Hundhausen Ulrich , Irbe Ilze , Jacobs Kordula , Klamer Morten , Kržišnik Davor , Lesar Boštjan , Melcher Eckhard , Meyer-Veltrup Linda , Morrell Jeffrey J. , Norton Jack , Palanti Sabrina , Presley Gerald , Reinprecht Ladislav , Singh Tripti , Stirling Rod , Venäläinen Martti , Westin Mats , Wong Andrew H. H. , Suttie Ed
Published: May 2021

Service life planning with timber requires reliable models for quantifying the effects of exposure-related parameters and the material-inherent resistance of wood against biotic agents. The Meyer-Veltrup model was the first attempt to account for inherent protective properties and the wetting ability of wood to quantify resistance of wood in a quantitative manner. Based on test data on brown, white, and soft rot as well as moisture dynamics, the decay rates of different untreated wood species were predicted relative to the reference species of Norway spruce (Picea abies). The present study aimed to validate and optimize the resistance model for a wider range of wood species including very durable species, thermally and chemically modified wood, and preservative treated wood. The general model structure was shown to also be suitable for highly durable materials, but previously defined maximum thresholds had to be adjusted (i.e., maximum values of factors accounting for wetting ability and inherent protective properties) to 18 instead of 5 compared to Norway spruce. As expected, both the enlarged span in durability and the use of numerous and partly very divergent data sources (i.e., test methods, test locations, and types of data presentation) led to a decrease in the predictive power of the model compared to the original. In addition to the need to enlarge the database quantity and improve its quality, in particular for treated wood, it might be advantageous to use separate models for untreated and treated wood as long as the effect of additional impact variables (e.g., treatment quality) can be accounted for. Nevertheless, the adapted Meyer-Veltrup model will serve as an instrument to quantify material resistance for a wide range of wood-based materials as an input for comprehensive service life prediction software.
Effect of Biomass-Burning Emissions on Soil Water Repellency: A Pilot Laboratory Study
Authors: Samburova Vera , Shillito Rose M. , Berli Markus , Khlystov Andrey Y. , Moosmüller Hans
Published: May 2021

While western U.S. wildfires have increased in intensity and scale, their impacts on soil chemical composition and hydraulic processes have received little attention, despite increasing erosion, surface runoff and flooding. The relationships between biomass-burning emissions, composition of the soil, fire heat, and soil water repellency are fragmental and sometimes contradictory. Here, we characterized the water repellency of sand (soil surrogate) exposed to Jeffrey pine duff smoke under controlled laboratory conditions. Water drop penetration time (WDPT), effective contact angle, and relative sorptivity of exposed silica sand (soil surrogate) were determined. Sand samples treated with smoke and heat or with cold smoke showed severe-to-extreme water repellency (WDPT > 1020 s). Sand samples exposed to fulvic acid (surrogate for soil organic matter) showed strong water repellency (WDPT = 81 s) that increased to become severe (WDPT = 2305 s) after subsequent heating to 200 °C for two hours. All sand samples exposed to either fire emissions or fulvic acid showed WDPTs >81 s, effective contact angles between 78° and 87°, and relative sorptivities between 0.31 and 0.49 compared with the untreated sand samples, with a WDPT <0.5 s, effective contact angle of 48°, and relative sorptivity of 1. This indicates that the sorptivity-controlled water infiltration into treated sand is less than half of that into untreated sand.
Exploring Generational Private Mobility Paradigm Shifts through Duration Modeling Analytics: A Greek Case Study
Authors: Fyrogenis Ioannis , Politis Ioannis
Published: May 2021

In this paper, we explore lifetime private mobility milestones in Greece and identify the factors that affect them, to explore the everchanging mobility landscape. In total, five archetypal private mobility milestones were examined: the age of getting a car driving license and the period until getting a car following that; the age of getting a motorbike driving license; the age of getting a first bicycle as an adult; and the age of first traveling by airplane. To this end, duration modeling and namely Kaplan-Meier and Cox Proportional Hazards models were developed. Results show that mobility paradigms are evolving and are affected by a wide array of factors. Generational differences are particularly highlighted, as younger travelers are less likely to get a car driving license or a car sooner but are more likely to get a bicycle as adults. Higher parents’ income diversely affects multiple mobility milestones. Growing up in rural locations and sustainable transport awareness also significantly affect mode choice related mobility milestones. Men were more likely to get both car and motorbike driving licenses at younger ages. The above results highlight the mobility profiles of Greek citizens and the factors that affect them, while offering insights into a future mobility landscape.
NGS-Based Diagnosis of Treatable Neurogenetic Disorders in Adults: Opportunities and Challenges
Authors: Good Jean-Marc , Atallah Isis , Castro Jimenez Mayte , Benninger David , Kuntzer Thierry , Superti-Furga Andrea , Tran Christel
Published: May 2021

The identification of neurological disorders by next-generation sequencing (NGS)-based gene panels has helped clinicians understand the underlying physiopathology, resulting in personalized treatment for some rare diseases. While the phenotype of distinct neurogenetic disorders is generally well-known in childhood, in adulthood, the phenotype can be unspecific and make the standard diagnostic approach more complex. Here we present three unrelated adults with various neurological manifestations who were successfully diagnosed using NGS, allowing for the initiation of potentially life-changing treatments. A 63-year-old woman with progressive cognitive decline, pyramidal signs, and bilateral cataract was treated by chenodeoxycholic acid following the diagnosis of cerebrotendinous xanthomatosis due to a homozygous variant in CYP27A1. A 32-year-old man with adult-onset spastic paraplegia, in whom a variant in ABCD1 confirmed an X-linked adrenoleukodystrophy, was treated with corticoids for adrenal insufficiency. The third patient, a 28-year-old woman with early-onset developmental delay, epilepsy, and movement disorders was treated with a ketogenic diet following the identification of a variant in SLC2A1, confirming a glucose transporter type 1 deficiency syndrome. This case study illustrates the challenges in the timely diagnosis of medically actionable neurogenetic conditions, but also the considerable potential for improving patient health through modern sequencing technologies.
Recentring Peripheral Queerness and Marginal Art in Portrait of a Lady on Fire (2019)
Authors: Pelling Madeleine
Published: May 2021

This essay examines the ways in which Céline Sciamma’s 2019 film Portrait de la Jeune Fille en Feu (Portrait of a Lady on Fire) looks to centralise onscreen homosexual experience through engagement with, and queering of, eighteenth-century art practices and the discourse surrounding them. From its reception of Ovid’s Metamorphoses to ideas espoused by the eighteenth-century art critic and philosopher Denis Diderot, Portrait looks to traditionally peripheral spaces, or edgelands, and the visual and embodied consequences of transcending them. Engaging closely with eighteenth-century processes of artmaking, the film transforms sketches on paper, paint applied to canvas and wood, miniatures held close to the body and erotica annotated in the margins into queer-coded sites used to reflect and document the developing relationship at its heart.
Cardiac Sarcoidosis: Pathophysiology, Diagnosis, and Management
Authors: Pour-Ghaz Issa , Kayali Sharif , Abutineh Iman , Patel Jay , Roman Sherif , Nayyar Mannu , Yedlapati Neeraja
Published: May 2021

Sarcoidosis is a chronic multi-system disorder with an unknown etiology that can affect the cardiac tissue, resulting in Cardiac Sarcoidosis (CS). The majority of these CS cases are clinically silent, and when there are symptoms, the symptoms are vague and can have a lot in common with other common cardiac diseases. These symptoms can range from arrhythmias to heart failure. If CS goes undetected, it can lead to detrimental outcomes for patients. Diagnosis depends on timely utilization of imaging modalities and non-invasive testing, while in some cases, it does necessitate biopsy. Early diagnosis and treatment with immunosuppressive agents are crucial, and it is essential that follow-up testing be performed to ensure resolution and remission. This manuscript provides an in-depth review of CS and the current literature regarding CS diagnosis and treatment.
STORAGE (STOchastic RAinfall GEnerator): A User-Friendly Software for Generating Long and High-Resolution Rainfall Time Series
Authors: De Luca Davide Luciano , Petroselli Andrea
Published: May 2021

The MS Excel file with VBA (Visual Basic for Application) macros named STORAGE (STOchastic RAinfall GEnerator) is introduced herein. STORAGE is a temporal stochastic simulator aiming at generating long and high-resolution rainfall time series, and it is based on the implementation of a Neymann–Scott Rectangular Pulse (NSRP) model. STORAGE is characterized by two innovative aspects. First, its calibration (i.e., the parametric estimation, on the basis of available sample data, in order to better reproduce some rainfall features of interest) is carried out by using data series (annual maxima rainfall, annual and monthly cumulative rainfall, annual number of wet days) which are usually longer than observed high-resolution series (that are mainly adopted in literature for the calibration of other stochastic simulators but are usually very short or absent for many rain gauges). Second, the seasonality is modelled using series of goniometric functions. This approach makes STORAGE strongly parsimonious with respect to the use of monthly or seasonal sets for parameters. Applications for the rain gauge network in the Calabria region (southern Italy) are presented and discussed herein. The results show a good reproduction of the rainfall features which are mainly considered for usual hydrological purposes.
Demographic and Cardiovascular Risk Factors Associated with Drug Use in Truck Drivers in the State of São Paulo, Brazil: A Cross-Sectional Study
Authors: Pereira Mariana Moura , Mansur Antonio de Padua , Takada Julio Yoshio , Leyton Vilma
Published: May 2021

The aim of the study is to analyze the association between risk factors for the health of truck drivers and previous use of illicit drugs. A cross-sectional study examined the data from 2071 truck drivers between 2010 and 2016. Demographic variables, risk factors for cardiovascular disease (CVD) and the use of illicit drugs were analyzed. The stepwise logistic regression model was used for the adjusted analysis. The dependent variable was the previous use of illicit drugs, and independent variables were those with p < 0.1 at a bivariate analysis. The average age of the truck drivers was 42.27 ± 11.07 years, and the previous use of illicit drugs was reported or detected in 388 (18.7%) drivers. Compared to non-users, drug users were younger (37.25 ± 9.45 vs. 43.43 ± 11.1 years; p < 0.001) and single (43.3% vs. 28.4%; p < 0.001). The independent variables for illicit drugs were age (OR = 0.93 (95% CI: 0.91–0.95; p < 0.001)), smoking (OR = 2.18 (95% CI: 1.39–3.44; p = 0.001)), alcohol consumption (OR = 1.626 (95% CI: 1.06–2.49; p = 0.026)) and driving hours per day (OR = 1.08 (95% CI: 1.01–1.15; p = 0.012)). Users of illicit drugs had multiple risk factors for CVD and traffic accidents.
Negative Impact of Fear of COVID-19 on Health-Related Quality of Life Was Modified by Health Literacy, eHealth Literacy, and Digital Healthy Diet Literacy: A Multi-Hospital Survey
Authors: Nguyen Minh H. , Pham Thu T. M. , Nguyen Kien T. , Nguyen Yen H. , Tran Tien V. , Do Binh N. , Dao Hung K. , Nguyen Huu C. , Do Ngoc T. , Ha Tung H. , Phan Dung T. , Pham Khue M. , Pham Linh V. , Nguyen Phuoc B. , Nguyen Hoai T. T. , Do Thinh V. , Ha Dung T. , Nguyen Hung Q. , Ngo Huong T. M. , Trinh Manh V. , Mai Thuy T. T. , Nguyen Nhan P. T. , Tra Anh L. , Nguyen Thao T. P. , Nguyen Kien T. , Bai Chyi-Huey , Duong Tuyen Van
Published: May 2021

Background: The COVID-19 pandemic has been disseminating fear in the community, which has affected people’s quality of life, especially those with health problems. Health literacy (HL), eHealth literacy (eHEAL), and digital healthy diet literacy (DDL) may have potential impacts on containing the pandemic and its consequences. This study aimed to examine the association between the fear of COVID-19 scale (FCoV-19S) and the health-related quality of life (HRQoL), and to examine the effect modification by HL, eHEAL, and DDL on this association. Methods: A cross-sectional study was conducted in 11 hospitals across Vietnam from 7 April to 31 May 2020. Data were collected on 4348 outpatients, including demographic characteristics, HL, eHEAL, DDL, FCoV-19S, and HRQoL. Multiple linear regression and interaction models were used to explore associations. Results: Patients with higher FCoV-19S scores had lower HRQoL scores (unstandardized coefficient, B = −0.78, p < 0.001). HL (B = 0.20, p < 0.001), eHEAL (B = 0.24, p < 0.001), and DDL (B = 0.20, p < 0.001) were positively associated with higher HRQoL scores. The negative impact of FCoV-19S on HRQoL was significantly attenuated by higher eHEAL score groups (from one standard deviation (SD) below the mean, B = −0.93, p < 0.001; to the mean, B = −0.85, p < 0.001; and one SD above the mean, B = −0.77, p < 0.001); and by higher DDL score groups (from one SD below the mean, B = −0.92, p < 0.001; to the mean, B = −0.82, p < 0.001; and one SD above the mean, B = −0.72, p < 0.001). Conclusions: eHealth literacy and digital healthy diet literacy could help to protect patients’ health-related quality of life from the negative impact of the fear of COVID-19 during the pandemic.
A Simple Electrostatic Precipitator for Trapping Virus Particles Spread via Droplet Transmission
Authors: Kakutani Koji , Matsuda Yoshinori , Nonomura Teruo , Takikawa Yoshihiro , Takami Takeshi , Toyoda Hideyoshi
Published: May 2021

The purpose of this study was to develop a simple electrostatic apparatus to precipitate virus particles spread via droplet transmission, which is especially significant in the context of the recent coronavirus disease 2019 (COVID-19) pandemic. The bacteriophage φ6 of Pseudomonas syringae was used as a model of the COVID-19 virus because of its similar structure and safety in experiments. The apparatus consisted of a spiked, perforated stainless plate (S-PSP) linked to a direct-current voltage generator to supply negative charge to the spike tips and a vessel with water (G-water) linked to a ground line. The S-PSP and G-water surface were paralleled at a definite interval. Negative charge supplied to the spike tips positively polarised the G-water by electrostatic induction to form an electric field between them in which ionic wind and negative ions were generated. Bacteriophage-containing water was atomised with a nebuliser and introduced into the electric field. The mist particles were ionised by the negative ions and attracted to the opposite pole (G-water). This apparatus demonstrated a prominent ability to capture phage-containing mist particles of the same sizes as respiratory droplets and aerosols regardless of the phage concentration of the mist particles. The trapped phages were successfully sterilised using ozone bubbling. Thus, the present study provides an effective system for eliminating droplet transmission of viral pathogens from public spaces.
Family Caregiver Suffering in Caring for Patients with Amyotrophic Lateral Sclerosis in Korea
Authors: Oh Juyeon , Kim Jung-A , Chu Min Sun
Published: May 2021

The purpose of this study was to describe the meaning of the suffering experience of Korean ALS family caregivers. This is a descriptive phenomenological study that included ten participants using convenience sampling with maximum variation in a tertiary hospital in Seoul, South Korea. Colaizzi’s data analysis method was used to inductively determine themes and formulate meanings. The three main themes derived from analysis were: “frustration with seeing a patient suffering”, “burnout at the cost of a life of dedication”, and “desperate need for help”. Caregivers experience high levels of suffering, which can come in various forms depending on the circumstances of the particular patient and family. Especially, distress from seeing a loved one suffering was another aspect of suffering in Korean ALS caregivers, reflecting strong family ties. At the same time, patients are in desperate need of help and support from their families. Thus, it is essential to provide care to lessen the causes of distress and meet the needs of not only patients, but also caregivers through family-centered care to improve overall quality of life for all involved.
Body Mapping in a Drug and Alcohol Treatment Program: Eliciting New Identity and Experience
Authors: Macken Sophia , Nathan Sally , Jersky Michelle , Boydell Katherine M. , Gibson Alexandra
Published: May 2021

Arts-based approaches have not been widely used in the drug and alcohol treatment sector. In this study, we examined the utility of the arts-based method of Body Mapping in an Australian residential treatment centre. Two workshops were held to explore young people’s strengths and support networks in order to improve understandings of young people’s lives, identities and experiences of treatment. Semi-structured follow-up interviews were conducted and triangulated with observational notes and staff interviews. We identified four major themes: engaging through art; removing the mask; revealing strengths; and a sense of achievement. Overall, this study highlighted the value of Body Mapping as an approach to engage with young people, providing rich in-depth data about their lived experiences, including in the alcohol and other drug (AOD) treatment context.
Estimation of Undetected Asymptomatic COVID-19 Cases in South Korea Using a Probabilistic Model
Authors: Lee Chanhee , Apio Catherine , Park Taesung
Published: May 2021

Increasing evidence shows that many infections of COVID-19 are asymptomatic, becoming a global challenge, since asymptomatic infections have the same infectivity as symptomatic infections. We developed a probabilistic model for estimating the proportion of undetected asymptomatic COVID-19 patients in the country. We considered two scenarios: one is conservative and the other is nonconservative. By combining the above two scenarios, we gave an interval estimation of 0.0001–0.0027 and in terms of the population, 5200–139,900 is the number of undetected asymptomatic cases in South Korea as of 2 February 2021. In addition, we provide estimates for total cases of COVID-19 in South Korea. Combination of undetected asymptomatic cases and undetected symptomatic cases to the number of confirmed cases (78,844 cases on 2 February 2021) shows that 0.17–0.42% (89,244–218,744) of the population have COVID-19. In conclusion, to control and understand the true ongoing reality of the pandemic, it is of outermost importance to focus on the ratio of undetected asymptomatic cases in the total population.
Chronic Ingestion of Bicarbonate-Rich Water Improves Anaerobic Performance in Hypohydrated Elite Judo Athletes: A Pilot Study
Authors: Chycki Jakub , Kostrzewa Maciej , Maszczyk Adam , Zajac Adam
Published: May 2021

In combat sports, anaerobic power and anaerobic capacity determine sport performance and the dominant metabolic pathways. The decline in performance during exercise that is attributed to the cumulative effects of fatigue, including excessive accumulation of metabolites, depletion of energy substrates, and water and electrolyte disturbances, seems to be of greatest significance. In our experiment, we evaluated the effectiveness of three weeks of bicarbonate-rich water ingestion on anaerobic performance in a state of hydration and dehydration in elite judo athletes. Eight male, elite judo athletes participated in two single-blind, repeated-measures trials. They were assigned to two hydration protocols, ingesting low mineralized table water and bicarbonate-rich water. Anaerobic performance was evaluated by two 30 s Wingate tests for lower and upper limbs, respectively, under conditions of hydration as well as exercise-induced dehydration. Resting, post-ingestion, and post-exercise concentrations of bicarbonate (HCO3), urine osmolality (UOSM), urine specific gravity (UGRAV), and lactate (La) were measured. The current investigation assessed two related factors that impair anaerobic performance—hypohydration and buffering capacity. High-bicarbonate water ingestion improved buffering capacity, and we demonstrated the potential role of this mechanism and its phenomenon in masking the adverse effects of dehydration in the context of repeated high-intensity anaerobic exercise (HIAE).
Respiratory Infections Following Earthquake-Induced Tsunamis: Transmission Risk Factors and Lessons Learned for Disaster Risk Management
Authors: Mavrouli Maria , Mavroulis Spyridon , Lekkas Efthymios , Tsakris Athanassios
Published: May 2021

Earthquake-induced tsunamis have the potential to cause extensive damage to natural and built environments and are often associated with fatalities, injuries, and infectious disease outbreaks. This review aims to examine the occurrence of respiratory infections (RIs) and to elucidate the risk factors of RI transmission following tsunamis which were induced by earthquakes in the last 20 years. Forty-seven articles were included in this review and referred to the RIs emergence following the 2004 Sumatra-Andaman, the 2009 Samoa, and the 2011 Japan earthquakes. Polymicrobial RIs were commonly detected among near-drowned tsunami survivors. Influenza outbreaks were commonly detected during the influenza transmission period. Overcrowded conditions in evacuation centers contributed to increased acute RI incidence rate, measles transmission, and tuberculosis detection. Destruction of health care infrastructures, overcrowded evacuation shelters, exposure to high pathogen densities, aggravating weather conditions, regional disease endemicity, and low vaccination coverage were the major triggering factors of RI occurrence in post-tsunami disaster settings. Knowledge of risk factors underlying RIs emergence following earthquake-induced tsunami can contribute to the implementation of appropriate disaster prevention and preparedness plans characterized by sufficient environmental planning, resistant infrastructures, resilient health care facilities, and well-established evacuation centers. Global and local disease surveillance is a key prerequisite for early warning and protection against RIs’ emergence and transmission in tsunami-prone areas.
Concurrent Heavy Metal Exposures and Idiopathic Dilated Cardiomyopathy: A Case-Control Study from the Katanga Mining Area of the Democratic Republic of Congo
Authors: Malamba-Lez Didier , Tshala-Katumbay Désire , Bito Virginie , Rigo Jean-Michel , Kipenge Kyandabike Richie , Ngoy Yolola Eric , Katchunga Philippe , Koba-Bora Béatrice , Ngoy-Nkulu Dophra
Published: May 2021

Blood and/or urine levels of 27 heavy metals were determined by ICPMS in 41 patients with dilated cardiomyopathy (DCM) and 29 presumably healthy subjects from the Katanga Copperbelt (KC), in the Democratic Republic of Congo (DRC). After adjusting for age, gender, education level, and renal function, DCM probability was almost maximal for blood concentrations above 0.75 and 150 µg/dL for arsenic and copper, respectively. Urinary concentrations above 1 for chromium, 20 for copper, 600 for zinc, 30 for selenium, 2 for cadmium, 0.2 for antimony, 0.5 for thallium, and 0.05 for uranium, all in μg/g of creatinine, were also associated with increased DCM probability. Concurrent and multiple exposures to heavy metals, well beyond permissible levels, are associated with increased probability for DCM. Study findings warrant screening for metal toxicity in case of DCM and prompt public health measures to reduce exposures in the KC, DRC.
New Insights into the Pathogenesis of Systemic Mastocytosis
Authors: Li Zhixiong
Published: May 2021

Mastocytosis is a type of myeloid neoplasm characterized by the clonal, neoplastic proliferation of morphologically and immunophenotypically abnormal mast cells that infiltrate one or more organ systems. Systemic mastocytosis (SM) is a more aggressive variant of mastocytosis with extracutaneous involvement, which might be associated with multi-organ dysfunction or failure and shortened survival. Over 80% of patients with SM carry the KIT D816V mutation. However, the KIT D816V mutation serves as a weak oncogene and appears to be a late event in the pathogenesis of mastocytosis. The management of SM is highly individualized and was largely palliative for patients without a targeted form of therapy in past decades. Targeted therapy with midostaurin, a multiple kinase inhibitor that inhibits KIT, has demonstrated efficacy in patients with advanced SM. This led to the recent approval of midostaurin by the United States Food and Drug Administration and European Medicines Agency. However, the overall survival of patients treated with midostaurin remains unsatisfactory. The identification of genetic and epigenetic alterations and understanding their interactions and the molecular mechanisms involved in mastocytosis is necessary to develop rationally targeted therapeutic strategies. This review briefly summarizes recent developments in the understanding of SM pathogenesis and potential treatment strategies for patients with SM.
Current Understanding of bHLH Transcription Factors in Plant Abiotic Stress Tolerance
Authors: Guo Jianrong , Sun Baixue , He Huanrong , Zhang Yifan , Tian Huaying , Wang Baoshan
Published: May 2021

Named for the characteristic basic helix-loop-helix (bHLH) region in their protein structure, bHLH proteins are a widespread transcription factor class in eukaryotes. bHLHs transcriptionally regulate their target genes by binding to specific positions on their promoters and thereby direct a variety of plant developmental and metabolic processes, such as photomorphogenesis, flowering induction, shade avoidance, and secondary metabolite biosynthesis, which are important for promoting plant tolerance or adaptation to adverse environments. In this review, we discuss the vital roles of bHLHs in plant responses to abiotic stresses, such as drought, salinity, cold, and iron deficiency. We suggest directions for future studies into the roles of bHLH genes in plant and discuss their potential applications in crop breeding.
The Potassium Transporter Hak1 in Candida Albicans, Regulation and Physiological Effects at Limiting Potassium and under Acidic Conditions
Authors: Ruiz-Castilla Francisco J. , Rodríguez-Castro Elisa , Michán Carmen , Ramos José
Published: May 2021

The three families of yeast plasma membrane potassium influx transporters are represented in Candida albicans: Trk, Acu, and Hak proteins. Hak transporters work as K+-H+ symporters, and the genes coding for Hak proteins are transcriptionally activated under potassium limitation. This work shows that C. albicans mutant cells lacking CaHAK1 display a severe growth impairment at limiting potassium concentrations under acidic conditions. This is the consequence of a defective capacity to transport K+, as indicated by potassium absorption experiments and by the kinetics parameters of Rb+ (K+) transport. Moreover, hak1− cells are more sensitive to the toxic cation lithium. All these phenotypes became much less robust or even disappeared at alkaline growth conditions. Finally, transcriptional studies demonstrate that the hak1− mutant, in comparison with HAK1+ cells, activates the expression of the K+/Na+ ATPase coded by CaACU1 in the presence of Na+ or in the absence of K+.
Assessing the Robustness of Cluster Solutions in Emotionally-Annotated Pictures Using Monte-Carlo Simulation Stabilized K-Means Algorithm
Authors: Horvat Marko , Jović Alan , Burnik Kristijan
Published: May 2021

Clustering is a very popular machine-learning technique that is often used in data exploration of continuous variables. In general, there are two problems commonly encountered in clustering: (1) the selection of the optimal number of clusters, and (2) the undecidability of the affiliation of border data points to neighboring clusters. We address both problems and describe how to solve them in application to affective multimedia databases. In the experiment, we used the unsupervised learning algorithm k-means and the Nencki Affective Picture System (NAPS) dataset, which contains 1356 semantically and emotionally annotated pictures. The optimal number of centroids was estimated, using the empirical elbow and silhouette rules, and validated using the Monte-Carlo simulation approach. Clustering with k = 1–50 centroids is reported, along with dominant picture keywords and descriptive statistical parameters. Affective multimedia databases, such as the NAPS, have been specifically designed for emotion and attention experiments. By estimating the optimal cluster solutions, it was possible to gain deeper insight into affective features of visual stimuli. Finally, a custom software application was developed for study in the Python programming language. The tool uses the scikit-learn library for the implementation of machine-learning algorithms, data exploration and visualization. The tool is freely available for scientific and non-commercial purposes.
Jorunnamycin A Suppresses Stem-Like Phenotypes and Sensitizes Cisplatin-Induced Apoptosis in Cancer Stem-Like Cell-Enriched Spheroids of Human Lung Cancer Cells
Authors: Sumkhemthong Somruethai , Chamni Supakarn , Ecoy Gea U. , Taweecheep Pornchanok , Suwanborirux Khanit , Prompetchara Eakachai , Chanvorachote Pithi , Chaotham Chatchai
Published: May 2021

It has been recognized that cancer stem-like cells (CSCs) in tumor tissue crucially contribute to therapeutic failure, resulting in a high mortality rate in lung cancer patients. Due to their stem-like features of self-renewal and tumor formation, CSCs can lead to drug resistance and tumor recurrence. Herein, the suppressive effect of jorunnamycin A, a bistetrahydroisoquinolinequinone isolated from Thai blue sponge Xestospongia sp., on cancer spheroid initiation and self-renewal in the CSCs of human lung cancer cells is revealed. The depletion of stemness transcription factors, including Nanog, Oct-4, and Sox2 in the lung CSC-enriched population treated with jorunnamycin A (0.5 μM), resulted from the activation of GSK-3β and the consequent downregulation of β-catenin. Interestingly, pretreatment with jorunnamycin A at 0.5 μM for 24 h considerably sensitized lung CSCs to cisplatin-induced apoptosis, as evidenced by upregulated p53 and decreased Bcl-2 in jorunnamycin A-pretreated CSC-enriched spheroids. Moreover, the combination treatment of jorunnamycin A (0.5 μM) and cisplatin (25 μM) also diminished CD133-overexpresssing cells presented in CSC-enriched spheroids. Thus, evidence on the regulatory functions of jorunnamycin A may facilitate the development of this marine-derived compound as a novel chemotherapy agent that targets CSCs in lung cancer treatment.
Challenges of Diagnosing Antibody-Mediated Rejection: The Role of Invasive and Non-Invasive Biomarkers
Authors: Krishnamoorthy Sambhavi , Kyeso Yousuf
Published: May 2021

Kidney transplantation is the best treatment modality for end-stage kidney disease, leading to improvement in a patient’s quality and quantity of life. With significant improvements in short-term outcomes, prolonging long-term allograft and patient survival remain ongoing challenges. The ability to monitor allograft function, immune tolerance and predict rejection accurately would enable personalization and better prognostication during post-transplant care. Though kidney biopsy remains the backbone of transplant diagnostics, emerging biomarkers can help detecting kidney allograft injury early enough to prevent permanent damage and detect injury before it is clinically apparent. In this review, we summarize the recent biomarkers that have shown promise in the prediction of acute rejection with a focus on antibody-mediated rejection in kidney transplantation.
A Rare Case of Tularemia Complicated by Rhabdomyolysis with a Successful Outcome
Authors: Kubiliute Ieva , Zablockiene Birute , Paulauskiene Rasute , Navickas Giedrius , Jancoriene Ligita
Published: May 2021

We present a case of tularemia complicated by rhabdomyolysis in a 43-year-old male who presented with fever, swelling, and pain of the right groin and a history of a week-old tick bite. Empirical parenteral amoxicillin/clavulanic acid treatment was initiated. Suspecting tularemia, parenteral gentamycin was added. Later, the patient started to complain of muscle pain, weakness, and difficulties in breathing and walking. Heightened levels of creatine kinase and myoglobin concentration (42,670 IU/L and >12,000 μg/L, respectively) were found. Due to rhabdomyolysis, large amounts of intravenous fluid therapy were initiated to prevent kidney damage, continuing intravenous antibiotic therapy. Francisella tularensis IgG in serum was found to be positive only on the sixteenth day of hospitalization. Upon discharge, the laboratory analyses returned to normal levels, and the patient was in good condition. The successful outcome could be associated with the early appropriate therapy of tularemia and its rare complication of rhabdomyolysis.
Remote Performance Modulation of Ultrafiltration Membranes by Magnetically and Thermally Responsive Polymer Chains
Authors: Sengupta Arijit , Vu Anh , Qian Xianghong , Wickramasinghe S. Ranil
Published: May 2021

Ultrafiltration membranes, that respond to an external magnetic field and local temperature have been developed. Surface-initiated activator-generated electron transfer (AGET) atom transfer radical polymerization (ATRP) has been used to graft poly(N-isopropylacrylamide) (PNIPAm) from the surface of 300 kDa regenerated cellulose membranes. The polymerization initiator was selectively attached to the entire membrane surface, only the outer membrane surface or only the inner pore surface. A superparamagnetic nanoparticle was attached to the end of the polymer chain. The DI water flux as well as the flux and rejection of bovine serum albumin were investigated in the absence and presence of a 20 and 1000 Hz oscillating magnetic field. In an oscillating magnetic field, the tethered superparamagnetic nanoparticles can cause movement of the PNIPAm chains or induce heating. A 20 Hz magnetic field maximizes movement of the chains. A 1000 Hz magnetic field leads to greater induced heating. PNIPAm displays a lower critical solution temperature at 32 °C. Heating leads to collapse of the PNIPAm chains above their Lower Critical Solution Temperature (LCST). This work highlights the versatility of selectively grafting polymer chains containing a superparamagnetic nanoparticle from specific membrane locations. Depending on the frequency of the oscillating external magnetic field, membrane properties may be tuned.
Oral Supplementation of an Alkylglycerol Mix Comprising Different Alkyl Chains Effectively Modulates Multiple Endogenous Plasmalogen Species in Mice
Authors: Paul Sudip , Rasmiena Aliki A. , Huynh Kevin , Smith Adam Alexander T. , Mellett Natalie A. , Jandeleit-Dahm Karin , Lancaster Graeme I. , Meikle Peter J.
Published: May 2021

Plasmalogens or alkenylphospholipids are a sub-class of glycerophospholipids with numerous biological functions and are thought to have protective effects against metabolic disease. Dietary supplementation with alkylglycerols (AKGs) has been shown to increase endogenous plasmalogen levels, however effective modulation of different molecular plasmalogen species has not yet been demonstrated. In this study, the effects of an orally-administered AKG mix (a mixture of chimyl, batyl and selachyl alcohol at a 1:1:1 ratio) on plasma and tissue lipids, including plasmalogens, was evaluated. Mice on a Western-type diet were treated with either an AKG mix or vehicle (lecithin) for 1, 2, 4, 8 and 12 weeks. Treatment with the AKG mix significantly increased the total plasmalogen content of plasma, liver and adipose tissue as a result of elevations in multiple plasmalogen species with different alkenyl chains. Alkylphospholipids, the endogenous precursors of plasmalogens, showed a rapid and significant increase in plasma, adipose tissue, liver and skeletal muscle. A significant accumulation of alkyl-diacylglycerol and lyso-ether phospholipids was also observed in plasma and tissues. Additionally, the dynamics of plasmalogen-level changes following AKG mix supplementation differed between tissues. These findings indicate that oral supplementation with an AKG mix is capable of upregulating and maintaining stable expression of multiple molecular plasmalogen species in circulation and tissues.
Wafer-Bonding Fabricated CMUT Device with Parylene Coating
Authors: He Changde , Zhang Binzhen , Xue Chenyang , Zhang Wendong , Zhang Shengdong
Published: May 2021

The advantages of the capacitive micromachined ultrasound transducer (CMUT) technology have provided revolutionary advances in ultrasound imaging. Extensive research on CMUT devices for high-frequency medical imaging applications has been conducted because of strong demands and fabrication realization by using standard silicon IC fabrication technology. However, CMUT devices for low-frequency underwater imaging applications have been rarely researched because it is difficult to fabricate thick membrane structures through depositing processes using standard IC fabrication technology due to stress-related problems. To address this shortcoming, in this paper, a CMUT device with a 2.83-μm thick silicon membrane is proposed and fabricated. The CMUT device is fabricated using silicon fusion wafer-bonding technology. A 5-μm thick Parylene-C is conformally deposited on the device for immersion measurement. The results show that the fabricated CMUT can transmit an ultrasound wave, receive an ultrasound wave, and have pulse-echo measurement capability. The ability of the device to emit and receive ultrasonic waves increases with the bias voltage but does not depend on the voltage polarity. The results demonstrate the viability of the fabricated CMUT in low-frequency applications from the perspectives of the device structure, fabrication, and characterization. This study presents the potential of the CMUT for underwater ultrasound imaging applications.
Thickness Optimization of Thin-Film Tandem Organic Solar Cell
Authors: Bangash Kamran Ali , Kazmi Syed Asfandyar Ali , Farooq Waqas , Ayub Saba , Musarat Muhammad Ali , Alaloul Wesam Salah , Javed Muhammad Faisal , Mosavi Amir
Published: May 2021

The polymer solar cells also known as organic solar cells (OSCs) have drawn attention due to their cynosure in industrial manufacturing because of their promising properties such as low weight, highly flexible, and low-cost production. However, low η restricts the utilization of OSCs for potential applications such as low-cost energy harvesting devices. In this paper, OSCs structure based on a triple-junction tandem scheme is reported with three different absorber materials to enhance the absorption of photons which in turn improves the η, as well as its correlating performance parameters. The investigated structure gives the higher value of η = 14.33% with Jsc = 16.87 (mA/m2), Voc = 1.0 (V), and FF = 84.97% by utilizing a stack of three different absorber layers with different band energies. The proposed structure was tested under 1.5 (AM) with 1 sun (W/m2). The impact of the top, middle, and bottom subcells’ thickness on η was analyzed with a terse to find the optimum thickness for three subcells to extract high η. The optimized structure was then tested with different electrode combinations, and the highest η was recorded with FTO/Ag. Moreover, the effect of upsurge temperature was also demonstrated on the investigated schematic, and it was observed that the upsurge temperature affects the photovoltaic (PV) parameters of the optimized cell and η decreases from 14.33% to 11.40% when the temperature of the device rises from 300 to 400 K.
Global Changes in Asexual Epichloë Transcriptomes during the Early Stages, from Seed to Seedling, of Symbiotum Establishment
Authors: Hettiarachchige Inoka K. , Vander Jagt Christy J. , Mann Ross C. , Sawbridge Timothy I. , Spangenberg German C. , Guthridge Kathryn M.
Published: May 2021

Asexual Epichloë fungi are strictly seed-transmitted endophytic symbionts of cool-season grasses and spend their entire life cycle within the host plant. Endophyte infection can confer protective benefits to its host through the production of bioprotective compounds. Inversely, plants provide nourishment and shelter to the resident endophyte in return. Current understanding of the changes in global gene expression of asexual Epichloë endophytes during the early stages of host-endophyte symbiotum is limited. A time-course study using a deep RNA-sequencing approach was performed at six stages of germination, using seeds infected with one of three endophyte strains belonging to different representative taxa. Analysis of the most abundantly expressed endophyte genes identified that most were predicted to have a role in stress and defence responses. The number of differentially expressed genes observed at early time points was greater than those detected at later time points, suggesting an active transcriptional reprogramming of endophytes at the onset of seed germination. Gene ontology enrichment analysis revealed dynamic changes in global gene expression consistent with the developmental processes of symbiotic relationships. Expression of pathway genes for biosynthesis of key secondary metabolites was studied comprehensively and fuzzy clustering identified some unique expression patterns. Furthermore, comparisons of the transcriptomes from three endophyte strains in planta identified genes unique to each strain, including genes predicted to be associated with secondary metabolism. Findings from this study highlight the importance of better understanding the unique properties of individual endophyte strains and will serve as an excellent resource for future studies of host-endophyte interactions.