bluuebunny/crossref_metadata_2025 huggingface dataset:
Wet Bone Characteristics Persist in Buried Bone after 10 Weeks: Implications for Forensic Anthropology
Authors: Maier Anna Katharina , Manzella Alessia , Bonicelli Andrea , Arnold Emily L. , Márquez-Grant Nicholas , Zioupos Peter
Published: August 2023

Assessing the timing of skeletal trauma significantly impacts the reconstruction of events surrounding death and deposition in forensic cases. However, there are no absolute time frames in which the characteristics of wet bone (peri-mortem) fractures transition to dry (post-mortem) fractures. The aim of this study was to attempt to identify a point within the post-mortem interval in which the characteristics of bone change from wet to dry bone properties. A total of 32 deer ribs were placed in a laboratory burial environment and a set of three were fractured with blunt force trauma every week during a ten-week period. All samples and the inflicted trauma effects were documented and analysed by macroscopic observation, scanning electron microscope (SEM) analysis, thermal analysis, biomechanical analysis, and attenuated total reflectance–Fourier transform infrared spectroscopy (ATR-FTIR). No significant difference was found in the macroscopic, microscopic, thermal, and biomechanical analyses of the trauma inflicted over the 10-week period. A significant difference was only found in the carbonate-to-phosphate ratio in analytical chemistry. The results suggest that interpreting wet bone characteristics in forensic anthropology as having been inflicted during the peri-mortem period (around the time of death) should also consider that these, in fact, could be inflicted well after death (post-mortem) as wet bone properties as this study has shown persist at least 10 weeks after death in a burial environment.
Approximate Analytical Solution of Fuzzy Linear Volterra Integral Equation via Elzaki ADM
Authors: Kapoor Mamta , Bin Turki Nasser , Shah Nehad Ali
Published: August 2023

In this paper, the fuzzy Volterra integral equations’ solutions are calculated using a hybrid methodology. The combination of the Elzaki transform and Adomian decomposition method results in the development of a novel regime. The precise fuzzy solutions are determined using Elzaki ADM after the fuzzy linear Volterra integral equations are first translated into two crisp integral equations utilizing the fuzzy number in parametric form. Three instances of the considered equations are solved to show the established scheme’s dependability, efficacy, and application. The results have a substantial impact on the fuzzy analytical dynamic equation theory. The comparison of the data in a graphical and tabular format demonstrates the robustness of the defined regime. The lower and upper bound solutions’ theoretical convergence and error estimates are highlighted in this paper. A tolerable order of absolute error is also obtained for this inquiry, and the consistency of the outcomes that are approximated and accurate is examined. The regime generated effective and reliable results. The current regime effectively lowers the computational cost, and a faster convergence of the series solution to the exact answer is signaled.
Technological Tools for the Conservation and Dissemination of Valencian Design Archives
Authors: Alba Ester , Gaitán Mar , León Arabella , Sevilla Javier , Solbes Álvaro , Pla Vicente
Published: August 2023

Design has shaped the world in which we live; it has improved our lives in ways we cannot even begin to imagine. Even if design is everywhere and is the memory of the world, collections associated with it are not usually well-known as design archives which capture spatial and temporal narratives. Saving these types of archives and making them accessible to the public, using them as cultural hubs, might improve our lives thanks to visual literacy, creativity, and innovation. The Arxiu Valencià del Disseny aims to recover, preserve, protect, and disseminate the archival cultural heritage of the Valencian designers. With a collection of more than 150,000 objects, it recovers history and evolution from the applied arts and product design, but with a markedly future-oriented character through the enhancement of the collections and their dissemination thanks to an intelligent computational system featuring cutting-edge technologies in order to prove our understanding of Valencian and European design. The aim of this paper is twofold: first, we introduce the AVD project, an interdisciplinary project that has been recently funded by the Valencian Government in order to preserve and promote the heritage of design archives; second, we introduce a set of interactive tools related to the project, such as the digitisation and cataloguing of the collection, the generation of a Design Memory Archive, advanced searching, and semantically relating the digitised collection of the Arxiu Valencià del Disseny based on data interoperability across its collections and a benchmarking tool for designers.
Biostimulants as a Means to Alleviate the Transplanting Shock in Lettuce
Authors: Krinis Dimitrios I. , Kasampalis Dimitrios S. , Siomos Anastasios S.
Published: August 2023

When transplanting vegetable plants in the field, the transplants undergo a small or large setback in growth, known as transplanting shock. Various practices are commonly applied to reduce the transplanting shock. In the past two decades, several studies have shown that the application of microbial and non-microbial biostimulants can enhance plant tolerance against abiotic stresses. However, there is no information on the effect of applying biostimulants at the time of transplanting in mitigating the transplanting shock of lettuce transplants in the field. Lettuce seedlings transplanted into the soil of an unheated plastic greenhouse were treated with two biostimulants, one microbial (Bactiva®) and one non-microbial (Isabion®), on the day of transplanting and after 14 and 21 days. During production, plant growth, and development, chlorophyll fluorescence and compositional parameters were determined. According to the results, the application of the non-microbial biostimulant had a significant effect on some measured parameters, with the leaves of the treated plants having a higher chlorophyll index (CCI) by 8%, as well as higher fluorescence parameters Fm/Fo, Fv/Fo, and Fv/Fm and area by 7, 10, 3, and 27%, respectively, but fewer total soluble phenols and lower fluorescence parameter ABS/RC by 7 and 26%, respectively, compared to the control untreated ones. The above may constitute some effects on the transplanting shock, without, however, being accompanied by significant effects on the number of leaves/plant, the leaf color parameters (L*, a*, b*, C*, and ho), and the chlorophyll (a, b, a + b), total carotenoid, dry matter, and nitrate content, along with the antioxidant capacity and plant fresh weight at harvest. However, a notable effect was that a greater percentage of plants at harvest had a fresh weight in the 351–400 class, while the greatest percentage of the control plants had a fresh weight in the 301–350 g class. In contrast, the application of the microbial biostimulant had no significant effect on any of the parameters determined compared to the control. Therefore, under the conditions of the present study, the effectiveness of biostimulant application at the time of transplanting on lettuce transplants is questionable.
Non-Invasive Risk Assessment and Prediction of Mortality in Patients Undergoing Coronary Artery Bypass Graft Surgery
Authors: Kim Ju-Youn , Park Young-Jun , Park Kyoung-Min , On Young-Keun , Kim June-Soo , Park Seung-Jung , Lee Young-Tak
Published: August 2023

Objectives: Heart rate turbulence (HRT) and T-wave alternans (TWA), non-invasive markers of cardiac autonomic dysfunction, and ventricular repolarization abnormality, reportedly, predict the risk of cardiovascular death after myocardial infarction. We investigated whether pre-operative assessment of HRT and/or TWA could predict long-term mortality following coronary artery bypass graft (CABG) surgery. Methods: From May 2010 to December 2017, patients undergoing elective CABG and receiving 24 h ambulatory electrocardiogram monitoring 1 to 5 days prior to CABG surgery were prospectively enrolled. Pre-operative HRT and TWA were measured using a 24 h ambulatory electrocardiogram. The relative risk of cardiac or overall death was assessed according to abnormalities of HRT, TWA, or left ventricular ejection fraction (LV EF). Results: During the mean follow-up period of 4.6 ± 3.9 years, 40 adjudicated overall (5.9%/yr) and 5 cardiac deaths (0.9%/yr) occurred in 146 enrolled patients (64.9 ± 9.3 years; 108 males). Patients with abnormal HRT exhibited significantly higher relative risks of cardiac death (adjusted hazard ratio [HR] 24.9, 95% confidence interval [CI] 1.46–427) and all-cause death (adjusted HR 5.77, 95% CI 2.34–14.2) compared to those with normal HRT. Moreover, abnormal HRT plus abnormal TWA and LV EF < 50% was associated with a greater elevation in cardiac and overall mortality risk. The predictive role of abnormal HRT with/without abnormal TWA for all-cause death was likely more prominent in patients with mildly reduced (35 to 50%) or preserved (≥50%) LV EF. Abnormal HRT plus abnormal TWA and LV EF < 50% showed high negative predictive value in cardiac and overall mortality risk. Conclusions: Assessment of pre-operative HRT and/or TWA predicted mortality risk in patients undergoing elective CABG. Combined analysis of HRT, TWA, and LVEF enhanced the prognostic power. In particular, the predictive value of HRT was enhanced in patients with preserved or mid-range LV EF.
Cardiac Morphology, Function, and Left Ventricular Geometric Pattern in Patients with Hypertensive Crisis: A Cardiovascular Magnetic Resonance-Based Study
Authors: Talle Mohammed A. , Doubell Anton F. , Robbertse Pieter-Paul S. , Lahri Sa’ad , Herbst Philip G.
Published: August 2023

(1) Background: Altered cardiac morphology and function are associated with increased risks of adverse cardiac events in hypertension. Our study aimed to assess left ventricular (LV) morphology, geometry, and function using cardiovascular magnetic resonance (CMR) imaging in patients with hypertensive crisis. (2) Methods: Patients with hypertensive crisis underwent CMR imaging at 1.5 Tesla to assess cardiac volume, mass, function, and contrasted study. Left ventricular (LV) function and geometry were defined according to the guideline recommendations. Late gadolinium enhancement (LGE) was qualitatively assessed and classified into ischemic and nonischemic patterns. Predictors of LGE was determined using regression analysis. (3) Results: Eighty-two patients with hypertensive crisis (aged 48.5 ± 13.4 years, and 57% males) underwent CMR imaging. Of these patients, seventy-eight percent were hypertensive emergency and twenty-two percent were urgency. Diastolic blood pressure was higher under hypertensive emergency (p = 0.032). Seventy-nine percent (92% of emergency vs. 59% of urgency, respectively; p = 0.003) had left ventricular hypertrophy (LVH). The most prevalent LV geometry was concentric hypertrophy (52%). Asymmetric LVH occurred in 13 (22%) of the participants after excluding ischemic LGE. Impaired systolic function occurred in 46% of patients, and predominantly involved hypertensive emergency. Nonischemic LGE occurred in 75% of contrasted studies (67.2% in emergency versus 44.4% in urgency, respectively; p < 0.001). Creatinine and LV mass were independently associated with nonischemic LGE. (5) Conclusion: LVH, altered geometry, asymmetric LVH, impaired LV systolic function, and LGE are common under hypertensive crisis. LVH and LGE more commonly occurred under hypertensive emergency. Longitudinal studies are required to determine the prognostic implications of asymmetric LVH and LGE in hypertensive crisis.
Cor Triatriatum Dexter Associated with an Ostium Primum Atrial Defect and Left-Sided Opening of the Coronary Sinus in a Stillborn Fetus
Authors: Iannaccone Silvia Farkašová , Sedmera David , Ginelliová Alžbeta , Bohuš Peter , Mistríková Lucia , Farkaš Daniel
Published: August 2023

Cor triatriatum is a very rare cardiac malformation characterized by the presence of an abnormal interatrial membrane separating either the left or right atrial chamber into two compartments. It can be associated with other cardiac defects and is often symptomatic in childhood. The signs depend on the size and position of the interatrial membrane and other associated malformations. Here we report a case of right-sided cor triatriatum associated with an ostium primum-type interatrial septum defect and left-sided opening of the coronary sinus in a fetus. The cause of intrauterine death was asphyxia due to total placental abruption.
Dicarboxylic Amino Acid Permease 7219 Regulates Fruiting Body Type of Auricularia heimuer
Authors: Lu Jia , Lu Lixin , Yao Fangjie , Fang Ming , Ma Xiaoxu , Meng Jingjing , Shao Kaisheng
Published: August 2023

Auricularia heimuer is a widely cultivated jelly mushroom. The fruiting bodies are categorized into cluster and chrysanthemum types. With changing consumer demands and the need to reduce bio-waste, the demand for clustered fruiting bodies is increasing. Therefore, gene mining for fruiting body types is a matter of urgency. We determined that the A. heimuer locus for fruiting body type was located at one end of the genetic linkage map. The locus was localized between the markers D23860 and D389 by increasing the density of the genetic linkage map. BlastN alignment showed that the marker SCL-18 was also located between D23860 and D389, and a total of 25 coding genes were annotated within this interval. Through parental transcriptome analysis and qRT-PCR verification, the locus g7219 was identified as the gene controlling the fruiting body type. A single-nucleotide substitution in the TATA box of g7219 was detected between the parents. By PCR amplification of the promoter region of g7219, the TATA-box sequences of the cluster- and chrysanthemum-type strains were found to be CATAAAA and TATAAAA, respectively. This study provides a foundation for the breeding of fruiting body types and strain improvement of A. heimuer.
Exploring Rural Resilient Factors Based on Spatial Resilience Theory: A Case Study of Southern Jiangsu
Authors: Yang Yiwei , Wang Yanhui
Published: August 2023

In the process of rapid urbanization in China, rural areas are facing increasingly complex changes and challenges. Resilience theory provides a multidimensional perspective of the sustainable development of rural regions. As a subset of the broader resilience framework, spatial resilience focuses on inter-component relationships and systematic characteristics at the spatial level. It projects the potential of resilience theory into the spatial domain of human habitats. This paper endeavors to integrate spatial resilience theory into the field of rural built environments. At the village level, relevant factors were extracted, and an exploratory analysis focusing on rural spatial resilience was conducted. Twenty-one villages in southern Jiangsu at various resilience levels were selected as empirical cases. Fuzzy-set qualitative comparative analysis (fsQCA) was employed to identify four configurations with sufficient conditions for rural spatial resilience. Furthermore, through an analysis of typical villages, the effective mechanisms of the relevant resilience factors were also elucidated. Our findings reveal several key points: (1) rural spatial resilience relies on an optimal combination of multiple factors rather than a single factor; (2) there are multiple potential pathways through which to enhance rural resilience; (3) and the configuration analysis of the rural factors of spatial resilience helps to narrow the distance between spatial resilience theory and spatial practice. This study validates and refines the application of spatial resilience theory in the context of the rural built environment. Corresponding suggestions are proposed for building a resilient countryside, aiming to provide support and reference for future development strategies in rural areas.
Changes in the Patterns of Population Distribution and Built-Up Areas of the Rural–Urban Fringe in Post-Socialist Context—A Central European Case Study
Authors: Pénzes János , Hegedűs László Dávid , Makhanov Kanat , Túri Zoltán
Published: August 2023

The rapid and significant expansion of urban areas is observed worldwide; however, considerable differences are detected within the characteristics of the process. The rural–urban fringe is changing most dynamically from the aspect of land use and this tends to be relevant in the case of post-socialist cities in Central Europe even with a stagnating or decreasing population. Debrecen (Hungary) and its hinterland adequately represent the migration trends of Hungarian cities and the great administrative area provided wide intra-urban suburbanization processes. The current study put the emphasis on the analysis of the spatial pattern of built-up areas and the distribution of residents. In order to discover the processes of the post-socialist transition period, detailed point layers were created to illustrate every built-up parcel in the rural–urban fringe of Debrecen (for the years 1980, 2000, and 2020). The most important characteristics were discovered with the help of GIS methods—Kernel-density, grid pattern analysis of the object density, and analysis of land cover/land use changes using Corine Land Cover Change (CLCC) databases. The dynamic and extended expansion of built-up areas was seen until 2000, in which the outskirts (including hobby gardens) densified spectacularly. The urban sprawl has been less intensive since the millennium and the increase in built-up areas has become more concentrated. As a consequence of the transition period, extended territories—primarily the least dense parts of the rural–urban fringe—are faced with the disappearance of buildings due to agricultural cultivation reasons.
The Interaction of the 1980 Child Abduction Convention with the Brussels II-ter Regulation: A Focus on the Regime of Recognition and Enforcement
Authors: Baruffi Maria Caterina
Published: August 2023

The paper addresses the interplay between the 1980 Child Abduction Convention and the Regulation (EU) 2019/1111, briefly presenting the main novelties contained in Chapter III of the Regulation devoted to international child abduction, and then focusing on the provisions concerning the peculiar regime of recognition and enforcement of decisions on this subject matter. Final considerations are drawn with a view to determining whether the Regulation is able to streamline the most critical issues arising from the practical application of the predecessor Regulation (EC) No 2201/2003 and, more broadly, to cope with evolving and challenging cases of child abduction.
Assessment of Biochemical and Neuroactivities of Cultural Filtrate from Trichoderma harzianum in Adjusting Electrolytes and Neurotransmitters in Hippocampus of Epileptic Rats
Authors: Abd El-Rahman Atef A. , El-Shafei Sally M. A. , Shehab Gaber M. G. , Mansour Lamjed , Abuelsaad Abdelaziz S. A. , Gad Rania A.
Published: August 2023

Background: Epilepsy is a serious chronic neurological disorder, which is accompanied by recurrent seizures. Repeated seizures cause physical injuries and neuronal dysfunction and may be a risk of cancer and vascular diseases. However, many antiepileptic drugs (AEDs) have side effects of mood alteration or neurocognitive function, a reduction in neuron excitation, and the inhibition of normal activity. Therefore, the present study aimed to evaluate the effect of secondary metabolites of Trichoderma harzianum cultural filtrate (ThCF) when adjusting different electrolytes and neurotransmitters in the hippocampus of epileptic rats. Methods: Cytotoxicity of ThCF against LS-174T cancer cells was assessed using a sulforhodamine B (SRB) assay. Quantitative estimation for some neurotransmitters, electrolytes in sera or homogenate of hippocampi tissues, and mRNA gene expression for ion or voltage gates was assessed by quantitative Real-Time PCR. Results: Treatment with ThCF reduces the proliferative percentage of LS-174T cells in a concentration-dependent manner. ThCF administration improves hyponatremia, hyperkalemia, and hypocalcemia in the sera of the epilepticus model. ThCF rebalances the elevated levels of many neurotransmitters and reduces the release of GABA and acetylcholine-esterase. Also, treatments with ThCF ameliorate the downregulation of mRNA gene expression for some gate receptors in hippocampal homogenate tissues and recorded a highly significant elevation in the expression of SCN1A, CACNA1S, and NMDA. Conclusion: Secondary metabolites of Trichoderma (ThCF) have cytotoxic activity against LS-174T (colorectal cancer cell line) and anxiolytic-like activity through a GABAergic mechanism of action and an increase in GABA as inhibitory amino acid in the selected brain regions and reduced levels of NMDA and DOPA. The present data suggested that ThCF may inhibit intracellular calcium accumulation by triggering the NAADP-mediated Ca2+ signaling pathway. Therefore, the present results suggested further studies on the molecular pathway for each metabolite of ThCF, e.g., 6-pentyl-α-pyrone (6-PP), harzianic acid (HA), and hydrophobin, as an alternative drug to mitigate the side effects of AEDs.
Review of Orbital Magnetism in Graphene-Based Moiré Materials
Authors: Jadaun Priyamvada , Soreé Bart
Published: August 2023

Recent years have seen the emergence of moiré materials as an attractive platform for observing a host of novel correlated and topological phenomena. Moiré heterostructures are generated when layers of van der Waals materials are stacked such that consecutive layers are slightly mismatched in their lattice orientation or unit cell size. This slight lattice mismatch gives rise to a long-wavelength moiré pattern that modulates the electronic structure and leads to novel physics. The moiré superlattice results in flat superlattice bands, electron–electron interactions and non-trivial topology that have led to the observation of superconductivity, the quantum anomalous Hall effect and orbital magnetization, among other interesting properties. This review focuses on the experimental observation and theoretical analysis of orbital magnetism in moiré materials. These systems are novel in their ability to host magnetism that is dominated by the orbital magnetic moment of Bloch electrons. This orbital magnetic moment is easily tunable using external electric fields and carrier concentration since it originates in the quantum anomalous Hall effect. As a result, the orbital magnetism found in moiré superlattices can be highly attractive for a wide array of applications including spintronics, ultra-low-power magnetic memories, spin-based neuromorphic computing and quantum information technology.
Evaluating the Efficacy of Water-Soluble Bone Wax (Tableau Wax) in Reducing Blood Loss in Spinal Fusion Surgery: A Randomized, Controlled, Pilot Study
Authors: Kim Jung Guel , Ham Dae-Woong , Zheng Haolin , Kwon Ohsang , Kim Ho-Joong
Published: August 2023

Background and Objectives: Lumbar decompression with fusion surgery is an effective treatment for spinal stenosis, but critical postoperative hematoma is a concern. Bone wax has been widely used to control bone bleeding but it has some drawbacks. This study aimed to evaluate the efficacy of Tableau wax, a bioabsorbable hemostatic material, in patients undergoing spinal fusion surgery through a pilot study design. Materials and Methods: A total of 31 patients were enrolled in this single-surgeon, single-institution study. The participants underwent transforaminal lumbar interbody fusion surgery and were randomly assigned to the control group (Bone wax) or test group (Tableau wax). Demographic data, pre- and post-operative hemoglobin levels, blood loss volume, surgical time, Oswestry Disability Index, and EQ-5D scores were recorded. Results: The study showed no significant difference in preoperative and postoperative hemoglobin levels, Oswestry Disability Index, and EQ-5D scores between the groups. However, the Tableau wax group had a significantly lower reduction in hemoglobin levels (1.3 ± 1.0 g/dL) and blood loss (438.2 mL) compared to the Bone wax group (2.2 ± 0.9 g/dL and 663.1 mL, respectively; p = 0.018 and p = 0.022).
Effects of an 11-Week Detraining, Imposed by the COVID-19 Confinement, on Handball Players’ Shoulder Rotator Isokinetic Profile, Shoulder Range of Motion, and Ball Release Velocity
Authors: Batalha Nuno , Sousa João Paulo , Fernandes Orlando , Dias Eduardo , Parraca Jose A. , Villafaina Santos
Published: August 2023

Background and Objectives: The COVID-19 confinement significantly impacted the physical condition of athletes. However, the detraining impacts of this period on the shoulder rotator and range of motion in handball players have not been studied. Thus, the main aim of this study was to investigate the effect of this 11-week detraining period, imposed by the COVID-19 pandemic confinement, on the shoulder rotator isokinetic profile (peak torque, ratio, fatigue index), shoulder rotator and flexion range of motion, and ball release velocity in handball players. Materials and Methods: A total of 16 handball players, with a mean age of 22.38 (5.28) years, participated in this study. The isokinetic strength was assessed using two protocols (three repetitions at an angular velocity of 60°/s and 20 repetitions at an angular velocity of 180°/s). In addition, the range of motion and ball release (at jump and standing shots) were measured. All these measurements were assessed before and after the COVID-19 confinement. Results: The results showed a significant reduction in the peak torque of the external rotation of their dominant and non-dominant shoulders. In addition, confinement significantly increased the fatigue index of external rotation and internal rotation and reduced the range of motion of internal rotation. Additionally, the ball release velocity during standing and jump shots was significantly reduced. Conclusions: These results suggested that strengthening external and internal rotation as well as recovering the internal rotation range of motion may be necessary after a detraining period in order to prevent shoulder injuries.
Role of Inflammatory Cytokines in Rheumatoid Arthritis and Development of Atherosclerosis: A Review
Authors: Bedeković Dražen , Bošnjak Ivica , Šarić Sandra , Kirner Damir , Novak Srđan
Published: August 2023

Uncontrolled chronic inflammation results in cardiovascular disease and early death. In this review, we studied the impact of rheumatoid arthritis on the cardiovascular system, including the early and accelerated development of atherosclerosis and its clinical manifestations, focusing on the inflammatory mechanisms leading to arterial wall damage, rapid atherosclerotic plaque formation, and thrombosis. Furthermore, the effect of medications used to treat rheumatoid arthritis on the cardiovascular system was studied. The effect of chronic inflammation and medication on traditional cardiovascular risk factors is not the main subject of this review. We observed that uncontrolled chronic inflammation and some medications directly impact all the stages of atherosclerosis. In conclusion, reducing inflammation and maintaining long-term remission in rheumatoid arthritis may prevent early atherosclerosis. We believe that this review will encourage a better interdisciplinary approach to the management of these patients and further research in this field.
Adenomyosis and Infertility: A Literature Review
Authors: Pados George , Gordts Stephan , Sorrentino Felice , Nisolle Michelle , Nappi Luigi , Daniilidis Angelos
Published: August 2023

Background and Objectives: Adenomyosis (the presence of ectopic endometrial glands and stroma below the endometrial–myometrial junction) is a benign condition which is increasingly diagnosed in younger women suffering from infertility. The aim of this narrative review was to study the pathophysiology and prevalence of adenomyosis, the mechanisms causing infertility, treatment options, and reproductive outcomes in infertile women suffering from adenomyosis. Materials and Methods: A literature search for suitable articles published in the English language was performed using PubMed from January 1970 to July 2022. Results: The literature search retrieved 50 articles that met the purpose of this review and summarized the most recent findings regarding the accuracy of diagnostic methods, pathophysiology, and the prevalence of adenomyosis and optimal strategies for the treatment of infertile women with adenomyosis. Conclusions: Adenomyosis is a common gynecological disorder, affecting women of reproductive age. It negatively affects in vitro fertilization, pregnancy and the live birth rate, as well as increases the risk of miscarriage. With the advent of non-invasive diagnoses with MRI and TVUS, the role of adenomyosis in infertility has been better recognized. Overall, more randomized controlled trials (RCTs) are needed to provide strong data on the accuracy of diagnostic methods, the pathophysiology and the prevalence of adenomyosis, the fertility outcomes of patients and the optimal strategy for the treatment.
Protein Phosphatase 2A as a Therapeutic Target in Pulmonary Diseases
Authors: Yu Howard , Zaveri Sahil , Sattar Zeeshan , Schaible Michael , Perez Gandara Brais , Uddin Anwar , McGarvey Lucas R. , Ohlmeyer Michael , Geraghty Patrick
Published: August 2023

New disease targets and medicinal chemistry approaches are urgently needed to develop novel therapeutic strategies for treating pulmonary diseases. Emerging evidence suggests that reduced activity of protein phosphatase 2A (PP2A), a complex heterotrimeric enzyme that regulates dephosphorylation of serine and threonine residues from many proteins, is observed in multiple pulmonary diseases, including lung cancer, smoke-induced chronic obstructive pulmonary disease, alpha-1 antitrypsin deficiency, asthma, and idiopathic pulmonary fibrosis. Loss of PP2A responses is linked to many mechanisms associated with disease progressions, such as senescence, proliferation, inflammation, corticosteroid resistance, enhanced protease responses, and mRNA stability. Therefore, chemical restoration of PP2A may represent a novel treatment for these diseases. This review outlines the potential impact of reduced PP2A activity in pulmonary diseases, endogenous and exogenous inhibitors of PP2A, details the possible PP2A-dependent mechanisms observed in these conditions, and outlines potential therapeutic strategies for treatment. Substantial medicinal chemistry efforts are underway to develop therapeutics targeting PP2A activity. The development of specific activators of PP2A that selectively target PP2A holoenzymes could improve our understanding of the function of PP2A in pulmonary diseases. This may lead to the development of therapeutics for restoring normal PP2A responses within the lung.
Future Perspectives in the Management of Otitis Media
Authors: Frosolini Andrea , Lovato Andrea
Published: August 2023

Otitis media, which encompasses acute otitis media (AOM) and chronic otitis media (COM), is a prevalent and significant health issue affecting both children and adults [...]
Monoclonal Antibodies Targeting CGRP: A Novel Treatment in Vestibular Migraine
Authors: Lovato Andrea , Disco Caterina , Frosolini Andrea , Monzani Daniele , Perini Francesco
Published: August 2023

Background. Monoclonal antibodies (mAbs) directed against the calcitonin gene-related peptide (CGRP) or its receptor represented the first targeted and specialized approach to migraine prophylaxis. Nevertheless, they have been rarely considered in the treatment of vestibular migraine (VM). Our aim was to evaluate the effectiveness of anti-CGRP mAbs in VM patients who did not respond to conventional migraine treatments. Methods. Consecutive VM patients treated with erenumab were considered. As a comparison, we considered the same VM patients during conventional migraine treatments (i.e., propranolol, flunarizine, or valproic acid), which were tried before mAbs therapy. Videonystagmography, the Italian version of the Dizziness Handicap Inventory (DHI) questionnaire, and migraine days over the last 3 months were evaluated in all patients before and after treatments. Results. In the present retrospective study, we included 21 female and 2 male VM patients, mean age 45.2 years. All patients underwent contrast-enhanced magnetic resonance imaging that ruled out other causes of vertigo. The DHI questionnaire significantly improved after mAb therapy (p < 0.0001). Mean migraine days over the last 3 months were significantly reduced after treatment (p = 0.001). Videonystagmography was altered in 11 (48%) patients prior to monoclonal antibodies. We found vertical positional nystagmus in 9 patients and horizontal positional nystagmus in 2 patients. After the treatment, we found vertical positional nystagmus only in 1 patient (p = 0.002). When patients were treated with conventional therapies, there was no significant reduction in DHI, and instrumental vestibular examinations remained altered. Conclusions. VM patients using anti-CGRP mAbs experienced a reduction in the dizziness-derived handicap, as reported in the DHI questionnaire. Furthermore, these treatments were significantly associated with a normalization of vestibular instrumental analysis. These findings were not seen with conventional treatments. Treatment with anti-CGRP mAbs may be effective in VM patients who did not respond to conventional migraine treatments. These findings should be tested in large, randomized clinical trials.
Geochemistry and Microbiology of Atacamite-Paratacamite Biofilms Floating on Underground Brine and Petroleum Pools in the White Pine Copper Mine, Michigan (USA)
Authors: Robbins Eleanora I. , Stanton Mark R. , Young Cheryl D.
Published: August 2023

At depth in an abandoned tunnel of the White Pine Copper Mine, green films of the Cu-OH-Cl minerals atacamite and paratacamite were found on standing pools of brine. Some pools were also coated with a thin layer of petroleum. Green films of atacamite were composed of individual blebs that averaged 20 μm in diameter and enclosed mixed colonies of Gram-negative, short rod-shaped, and sheathed filamentous bacteria. Carbon δ13C values in the atacamite–paratacamite mixtures reflect the isotopic values of bacteria and minor amounts of petroleum mixed with the minerals. Heterotrophic bacteria are interpreted to be using petroleum as a carbon source and may be catalyzing the precipitation of the copper hydroxy chloride minerals or acting as a template.
Lysozyme Modification Using Proteolytic Enzymes
Authors: Tomczyk Łukasz , Leśnierowski Grzegorz , Cegielska-Radziejewska Renata
Published: August 2023

The lysozyme in the chicken egg white consists of various bioactive amino acids. However, these compounds are inactive when they are in the sequence of parent proteins. They become active only when isolated from these proteins. The aim of this study was to modify lysozyme with proteolytic enzymes under specific conditions of the reaction environment so as to obtain active biopeptides. The physicochemical properties of the resulting preparations were also assessed. Our study showed that the modification of lysozyme with hydrolytic enzymes (pepsin and trypsin) under strictly specified conditions resulted in obtaining biopeptide preparations with new and valuable properties, as compared with native lysozyme. After the enzymatic modification of lysozyme, two structural fractions were distinguished in the composition of the resulting preparations—the monomeric fraction and the peptide fraction. The modified lysozyme exhibited high surface hydrophobicity and high total antibacterial activity despite the decrease in the hydrolytic activity. Modification of lysozyme with hydrolytic enzymes, especially pepsin, resulted in preparations with very good antioxidative properties.
Functionalization of ZnAl-Layered Double Hydroxide with Ensulizole and Its Application as a UV-Protective Agent in a Transparent Polymer Coating
Authors: Melánová Klára , Kopecká Kateřina , Beneš Ludvík , Kutálek Petr , Knotek Petr , Zmrhalová Zuzana , Svoboda Jan
Published: August 2023

In this study, we propose a promising photoprotective additive that combines the advantages of both organic UV absorbers and inorganic particles without compromising the properties of the paint material. This additive involves the intercalation of a well-known organic UV absorber, 2-phenylbenzimidazole-5-sulfonic acid (PBISA), into zinc-aluminum layered double hydroxide (ZnAl-LDH). Three ZnAl-LDH intercalates with PBISA were prepared using various methods based on either anion exchange or direct synthesis. The intercalates were characterized using powder X-ray diffraction, thermogravimetry, elemental analysis, and IR and UV-Vis spectroscopies. The composition and basal spacings of all three intercalates are very similar. An effective UV protection film was prepared when the ZnAl–PBISA–1 intercalate was incorporated into polyurethane-acrylate lacquer. The resultant UV protective film exhibited stability and uniform distribution of the intercalated fillers. Some minimal particle sedimentation and aggregation were observed on the cured film’s underside, but did not compromise the films’ UV protective properties. The prepared lacquers with intercalated fillers offer a viable solution for the surface modification of plastic products.
On the Potential Role of the (Pseudo-) Jahn–Teller Effect in the Membrane Transport Processes: Enniatin B and Beauvericin
Authors: Štellerová Dagmar , Lukeš Vladimír , Breza Martin
Published: August 2023

The molecular structure of mycotoxins enniatin B and beauvericin, which are used as ionophores, was studied using density functional theory in various symmetry groups and singly charged states. We have shown that the charge addition or removal causes significant structural changes. Unlike the neutral C3 molecules, the stability of the charged C1 structures was explained by the Jahn–Teller or Pseudo-Jahn–Teller effect. This finding agrees with the available experimental X-ray structures of their metal complexes where electron density transfer from the metal can be expected. Hence, the membrane permeability of metal sandwich-structure complexes possessing antimicrobial activities is modulated by the conformational changes.
New Advances in the Exploration of Esterases with PET and Fluorescent Probes
Authors: Gil-Rivas Alba , de Pascual-Teresa Beatriz , Ortín Irene , Ramos Ana
Published: August 2023

Esterases are hydrolases that catalyze the hydrolysis of esters into the corresponding acids and alcohols. The development of fluorescent probes for detecting esterases is of great importance due to their wide spectrum of biological and industrial applications. These probes can provide a rapid and sensitive method for detecting the presence and activity of esterases in various samples, including biological fluids, food products, and environmental samples. Fluorescent probes can also be used for monitoring the effects of drugs and environmental toxins on esterase activity, as well as to study the functions and mechanisms of these enzymes in several biological systems. Additionally, fluorescent probes can be designed to selectively target specific types of esterases, such as those found in pathogenic bacteria or cancer cells. In this review, we summarize the recent fluorescent probes described for the visualization of cell viability and some applications for in vivo imaging. On the other hand, positron emission tomography (PET) is a nuclear-based molecular imaging modality of great value for studying the activity of enzymes in vivo. We provide some examples of PET probes for imaging acetylcholinesterases and butyrylcholinesterases in the brain, which are valuable tools for diagnosing dementia and monitoring the effects of anticholinergic drugs on the central nervous system.
Characteristics of Phenolic Compounds in Peucedanum japonicum According to Various Stem and Seed Colors
Authors: Lee Chang-Dae , Cho Hyejin , Shim Jeehyoung , Tran Gia Han , Lee Hak-Dong , Ahn Kwang Hoon , Yoo Eunae , Chung Mi Ja , Lee Sanghyun
Published: August 2023

Total polyphenol and total flavonoid assays were performed to characterize the relationships between the color of Peucedanum japonicum (PJ) seed coat and stem and the content of phytochemical compounds. The samples were divided into two groups based on their stem and seed coat color, with each group containing 23 samples. The stem color group was subdivided into green, light red, and red, whereas the seed coat color group was divided into light brown, brown, and dark brown. In the stem color group, the light red stems exhibited the highest content of phytochemical compounds, with levels over 10% higher than those of the stems of the other colors. Moreover, among the top ten samples with the highest total polyphenol content, eight samples were light red, and the light red group also exhibited the highest total flavonoid content among the examined color groups. In terms of the seed coat color, the plants grown from dark brown seeds exhibited the highest contents of both total polyphenols and total flavonoids. In conclusion, PJ plants with dark brown seeds and light red stems contained the highest levels of phytochemical compounds. Collectively, our findings provide a valuable basis for future seed selection of PJ for pharmaceutical purposes.
Nucleoside Analog Reverse-Transcriptase Inhibitors in Membrane Environment: Molecular Dynamics Simulations
Authors: Stachowicz-Kuśnierz Anna , Korchowiec Beata , Korchowiec Jacek
Published: August 2023

The behavior of four drugs from the family of nucleoside analog reverse-transcriptase inhibitors (zalcitabine, stavudine, didanosine, and apricitabine) in a membrane environment was traced using molecular dynamics simulations. The simulation models included bilayers and monolayers composed of POPC and POPG phospholipids. It was demonstrated that the drugs have a higher affinity towards POPG membranes than POPC membranes due to attractive long-range electrostatic interactions. The results obtained for monolayers were consistent with those obtained for bilayers. The drugs accumulated in the phospholipid polar headgroup region. Two adsorption modes were distinguished. They differed in the degree of penetration of the hydrophilic headgroup region. Hydrogen bonds between drug molecules and phospholipid heads were responsible for adsorption. It was shown that apricitabine penetrated the hydrophilic part of the POPC and POPG membranes more effectively than the other drugs. Van der Waals interactions between S atoms and lipids were responsible for this.
Exquisitely Constructing a Robust MOF with Dual Pore Sizes for Efficient CO2 Capture
Authors: Li Yanxi , Bai Yuhua , Wang Zhuozheng , Gong Qihan , Li Mengchen , Bo Yawen , Xu Hua , Jiang Guiyuan , Chi Kebin
Published: August 2023

Developing metal–organic framework (MOF) adsorbents with excellent performance and robust stability is of critical importance to reduce CO2 emissions yet challenging. Herein, a robust ultra-microporous MOF, Cu(bpfb)(bdc), with mixed ligands of N, N′-(1,4-phenylene)diisonicotinamide (bpfb), and 1,4-dicarboxybenzene (bdc) was delicately constructed. Structurally, this material possesses double-interpenetrated frameworks formed by two staggered, independent frameworks, resulting in two types of narrow ultra-micropores of 3.4 × 5.0 and 4.2 × 12.8 Å2, respectively. The above structural properties make its highly selective separation at 273~298 K with a CO2 capacity of 71.0~86.2 mg/g. Its adsorption heat over CO2 and IAST selectivity were calculated to be 27 kJ/mol and 52.2, respectively. Remarkably, cyclic breakthrough experiments corroborate its impressive performance in CO2/N2 separation in not only dry but also 75% RH humid conditions. Molecular simulation reveals that C-H···OCO2 in the pores plays a pivotal role in the high selectivity of CO2 adsorption. These results point out the huge potential application of this material for CO2/N2 separation.
Role of Delocalization, Asymmetric Distribution of π-Electrons and Elongated Conjugation System for Enhancement of NLO Response of Open Form of Spiropyran-Based Thermochromes
Authors: Kosar Naveen , Kanwal Saba , Hamid Malai Haniti S. A. , Ayub Khurshid , Gilani Mazhar Amjad , Imran Muhammad , Arshad Muhammad , Alkhalifah Mohammed A. , Sheikh Nadeem S. , Mahmood Tariq
Published: August 2023

Switchable nonlinear optical (NLO) materials have widespread applications in electronics and optoelectronics. Thermo-switches generate many times higher NLO responses as compared to photo-switches. Herein, we have investigated the geometric, electronic, and nonlinear optical properties of spiropyranes thermochromes via DFT methods. The stabilities of close and open isomers of selected spiropyranes are investigated through relative energies. Electronic properties are studied through frontier molecular orbitals (FMOs) analysis. The lower HOMO-LUMO energy gap and lower excitation energy are observed for open isomers of spiropyranes, which imparts the large first hyperpolarizability value. The delocalization of π-electrons, asymmetric distribution and elongated conjugation system are dominant factors for high hyperpolarizability values of open isomers. For deep understanding, we also analyzed the frequency-dependent hyperpolarizability and refractive index of considered thermochromes. The NLO response increased significantly with increasing frequency. Among all those compounds, the highest refractive index value is observed for the open isomer of the spiropyran 1 (1.99 × 10−17 cm2/W). Molecular absorption analysis confirmed the electronic excitation in the open isomers compared to closed isomers. The results show that reversible thermochromic compounds act as excellent NLO molecular switches and can be used to design advanced electronics.
A Critical Review of the Enhanced Recovery of Rare Earth Elements from Phosphogypsum
Authors: Xie Gang , Guan Qingjun , Zhou Fujia , Yu Weijian , Yin Zhigang , Tang Honghu , Zhang Zhenyue , Chi Ru’an
Published: August 2023

The increasing demand for rare earth elements (REEs), especially from new and innovative technology, has strained their supply, which makes the exploration of new REE sources necessary, for example, the recovery of REEs from phsophogypsum (PG). PG is a byproduct during the wet production of phosphoric acid, which is an attractive secondary resource for REEs due to a large amount of REEs locked in them. In most cases, REEs contained in PG are mainly encapsulated in the gypsum crystal, leading to a low leaching efficiency. Therefore, it is particularly important to use various methods to enhance the leaching of REEs from PG. In this review, we summarized and classified various enhanced leaching methods for the recovery of REEs from PG, and the advantages and disadvantages of different methods were compared. A joint method of recrystallization and RIL may be a promising enhanced leaching approach for the recovery of REEs from PG. Recrystallization could achieve both the complete REE release and simultaneous preparation of industrial materials with high value added, such as high-strength α-hemihydrate gypsum by phase transformation of PG, and the RIL technology could adsorb the releasing REEs and realize their efficient extraction. Such a combination appears to show significant advantages because of high REE recovery, as well as high value-added product preparation at low cost.