bluuebunny/crossref_metadata_2025 huggingface dataset:
Interaction of the Transcription Factors BES1/BZR1 in Plant Growth and Stress Response
Authors: Cao Xuehua , Wei Yanni , Shen Biaodi , Liu Linchuan , Mao Juan
Published: June 2024

Bri1-EMS Suppressor 1 (BES1) and Brassinazole Resistant 1 (BZR1) are two key transcription factors in the brassinosteroid (BR) signaling pathway, serving as crucial integrators that connect various signaling pathways in plants. Extensive genetic and biochemical studies have revealed that BES1 and BZR1, along with other protein factors, form a complex interaction network that governs plant growth, development, and stress tolerance. Among the interactome of BES1 and BZR1, several proteins involved in posttranslational modifications play a key role in modifying the stability, abundance, and transcriptional activity of BES1 and BZR1. This review specifically focuses on the functions and regulatory mechanisms of BES1 and BZR1 protein interactors that are not involved in the posttranslational modifications but are crucial in specific growth and development stages and stress responses. By highlighting the significance of the BZR1 and BES1 interactome, this review sheds light on how it optimizes plant growth, development, and stress responses.
Synaptic Mechanisms of Ethanol Tolerance and Neuroplasticity: Insights from Invertebrate Models
Authors: Bhandari Aakriti , Seguin Alexandra , Rothenfluh Adrian
Published: June 2024

Alcohol tolerance is a neuroadaptive response that leads to a reduction in the effects of alcohol caused by previous exposure. Tolerance plays a critical role in the development of alcohol use disorder (AUD) because it leads to the escalation of drinking and dependence. Understanding the molecular mechanisms underlying alcohol tolerance is therefore important for the development of effective therapeutics and for understanding addiction in general. This review explores the molecular basis of alcohol tolerance in invertebrate models, Drosophila and C. elegans, focusing on synaptic transmission. Both organisms exhibit biphasic responses to ethanol and develop tolerance similar to that of mammals. Furthermore, the availability of several genetic tools makes them a great candidate to study the molecular basis of ethanol response. Studies in invertebrate models show that tolerance involves conserved changes in the neurotransmitter systems, ion channels, and synaptic proteins. These neuroadaptive changes lead to a change in neuronal excitability, most likely to compensate for the enhanced inhibition by ethanol.
Comparative Transcriptome Analysis Reveals Key Functions of MiMYB Gene Family in Macadamia Nut Pericarp Formation
Authors: Tan Qiujin , Huan Xiuju , Pan Zhenzhen , Yang Xiaozhou , Wei Yuanrong , Zhou Chunheng , Wang Wenlin , Wang Lifeng
Published: June 2024

Macadamia nuts are one of the most important economic food items in the world. Pericarp thickness and flavonoid composition are the key quality traits of Macadamia nuts, but the underlying mechanism of pericarp formation is still unknown. In this study, three varieties with significantly different pericarp thicknesses, namely, A38, Guire No.1, and HAES 900, at the same stage of maturity, were used for transcriptome analysis, and the results showed that there were significant differences in their gene expression profile. A total of 3837 new genes were discovered, of which 1532 were functionally annotated. The GO, COG, and KEGG analysis showed that the main categories in which there were significant differences were flavonoid biosynthesis, phenylpropanoid biosynthesis, and the cutin, suberine, and wax biosynthesis pathways. Furthermore, 63 MiMYB transcription factors were identified, and 56 R2R3-MYB transcription factors were clustered into different subgroups compared with those in Arabidopsis R2R3-MYB. Among them, the S4, S6, and S7 subgroups were involved in flavonoid biosynthesis and pericarp formation. A total of 14 MiMYBs’ gene expression were verified by RT-qPCR analysis. These results provide fundamental knowledge of the pericarp formation regulatory mechanism in macadamia nuts.
WRKY Transcription Factor Responses and Tolerance to Abiotic Stresses in Plants
Authors: Ma Ziming , Hu Lanjuan
Published: June 2024

Plants are subjected to abiotic stresses throughout their developmental period. Abiotic stresses include drought, salt, heat, cold, heavy metals, nutritional elements, and oxidative stresses. Improving plant responses to various environmental stresses is critical for plant survival and perpetuation. WRKY transcription factors have special structures (WRKY structural domains), which enable the WRKY transcription factors to have different transcriptional regulatory functions. WRKY transcription factors can not only regulate abiotic stress responses and plant growth and development by regulating phytohormone signalling pathways but also promote or suppress the expression of downstream genes by binding to the W-box [TGACCA/TGACCT] in the promoters of their target genes. In addition, WRKY transcription factors not only interact with other families of transcription factors to regulate plant defence responses to abiotic stresses but also self-regulate by recognising and binding to W-boxes in their own target genes to regulate their defence responses to abiotic stresses. However, in recent years, research reviews on the regulatory roles of WRKY transcription factors in higher plants have been scarce and shallow. In this review, we focus on the structure and classification of WRKY transcription factors, as well as the identification of their downstream target genes and molecular mechanisms involved in the response to abiotic stresses, which can improve the tolerance ability of plants under abiotic stress, and we also look forward to their future research directions, with a view of providing theoretical support for the genetic improvement of crop abiotic stress tolerance.
Effects of High-Mobility Group Box-1 on Mucosal Immunity and Epithelial Differentiation in Colitic Carcinoma
Authors: Sasaki Takamitsu , Fujiwara-Tani Rina , Luo Yi , Ogata Ruiko , Sasaki Rika , Ikemoto Ayaka , Nishiguchi Yukiko , Nakashima Chie , Kishi Shingo , Fujii Kiyomu , Ohmori Hitoshi , Oue Naohide , Kuniyasu Hiroki
Published: June 2024

Abnormalities in mucosal immunity are involved in the onset and progression of ulcerative colitis (UC), resulting in a high incidence of colorectal cancer (CRC). While high-mobility group box-1 (HMGB1) is overexpressed during colorectal carcinogenesis, its role in UC-related carcinogenesis remains unclear. In the present study, we investigated the role of HMGB1 in UC-related carcinogenesis and sporadic CRC. Both the azoxymethane colon carcinogenesis and dextran sulfate sodium colitis carcinogenesis models demonstrated temporal increases in mucosal HMGB1 levels. Activated CD8+ cells initially increased and then decreased, whereas exhausted CD8+ cells increased. Additionally, we observed increased regulatory CD8+ cells, decreased naïve CD8+ cells, and decreased mucosal epithelial differentiation. In the in vitro study, HMGB1 induced energy reprogramming from oxidative phosphorylation to glycolysis in CD8+ cells and intestinal epithelial cells. Furthermore, in UC dysplasia, UC-related CRC, and hyperplastic mucosa surrounding human sporadic CRC, we found increased mucosal HMGB1, decreased activated CD8+ cells, and suppressed mucosal epithelial differentiation. However, we observed increased activated CD8+ cells in active UC mucosa. These findings indicate that HMGB1 plays an important role in modulating mucosal immunity and epithelial dedifferentiation in both UC-related carcinogenesis and sporadic CRC.
Comprehensive Analysis of the Function and Prognostic Value of TAS2Rs Family-Related Genes in Colon Cancer
Authors: Bi Suzhen , Zhu Jie , Huang Liting , Feng Wanting , Peng Lulu , Leng Liangqi , Wang Yin , Shan Peipei , Kong Weikaixin , Zhu Sujie
Published: June 2024

In the realm of colon carcinoma, significant genetic and epigenetic diversity is observed, underscoring the necessity for tailored prognostic features that can guide personalized therapeutic strategies. In this study, we explored the association between the type 2 bitter taste receptor (TAS2Rs) family-related genes and colon cancer using RNA-sequencing and clinical datasets from The Cancer Genome Atlas (TCGA) and the Gene Expression Omnibus (GEO). Our preliminary analysis identified seven TAS2Rs genes associated with survival using univariate Cox regression analysis, all of which were observed to be overexpressed in colon cancer. Subsequently, based on these seven TAS2Rs prognostic genes, two colon cancer molecular subtypes (Cluster A and Cluster B) were defined. These subtypes exhibited distinct prognostic and immune characteristics, with Cluster A characterized by low immune cell infiltration and less favorable outcomes, while Cluster B was associated with high immune cell infiltration and better prognosis. Finally, we developed a robust scoring system using a gradient boosting machine (GBM) approach, integrated with the gene-pairing method, to predict the prognosis of colon cancer patients. This machine learning model could improve our predictive accuracy for colon cancer outcomes, underscoring its value in the precision oncology framework.
Impact of Tomato Grafts on the Potato Tuber Metabolome and Skin Colour
Authors: Villányi Vanda , Odgerel Khongorzul , Okaroni Cathrine O. , Bánfalvi Zsófia
Published: June 2024

Grafting is a traditional research and crop production technique used to study the long-distance movement of molecules, reduce disease susceptibility, and improve yield, quality, and nutrient content. Tomato/potato grafts are rare examples of successful interspecies grafting, even resulting in commercially available products. Nevertheless, information on the effect of tomato on the quality parameters of potato tubers is scarce. In this study, the tomato cultivar ‘Mobil’ was grafted with the potato cultivars ‘White Lady’, ‘Hópehely’, and ‘Désirée’, and the phenotype, metabolite composition, and starch and protein contents of the tubers were analysed. Anthocyanins were isolated from the tuber skins, and the expression level of the transcription factor ANTHOCYANIN1 (StAN1) was evaluated. Out of the 112 identified metabolites, the concentrations of twelve compounds were altered in the same direction in all three cultivars. Compared to the self-grafted control, the starch content of tubers was increased in each cultivar, while the protein level remained unaltered in ‘White Lady’ and ‘Hópehely’. The oval tubers became roundish. The tomato scion increased the anthocyanin content of ‘Hópehely’ and ‘Désirée’ tuber skins, which was correlated with the upregulation of StAN1 expression. These results indicate that tomato scion has a significant impact on the quality parameters of potato tubers.
Automated Knowledge Extraction in the Field of Wheat Sharp Eyespot Control
Authors: Liu Keyi , Cui Yunpeng
Published: June 2024

Wheat sharp eyespot is a soil-borne fungal disease commonly found in wheat areas in China, which can occur throughout the entire reproductive period of wheat and has a great impact on the yield and quality of wheat in China. By constructing a domain ontology for wheat sharp eyespot control and modeling the domain knowledge, we aim to integrate and share the knowledge in the field of wheat sharp eyespot control, which can provide important support and guidance for agricultural decision-making and disease control. In this study, the literature in the field of wheat sharp eyespot control was used as a data source, the KeyBERT keyword extraction algorithm was used to mine the core concepts of the ontology, and the hierarchical relationships among the ontology concepts were extracted through clustering. Based on the constructed ontology of wheat sharp eyespot control, the schema of knowledge extraction was formed, and the knowledge extraction model was trained using the ERNIE 3.0 knowledge enhancement pretraining model. This study proposes a model and algorithm to realize knowledge extraction based on domain ontology, describes the construction method and process framework of wheat sharp eyespot control domain ontology, and details the training and reasoning effect of the knowledge extraction model. The knowledge extraction model constructed in this study for wheat sharp eyespot control contains a more complete conceptual system of wheat sharp eyespot. The F1 value of the model reaches 91.26%, which is a 17.86% improvement compared with the baseline model, and it can satisfy the knowledge extraction needs in the field of wheat sharp eyespot control. This study can provide a reference for domain knowledge extraction and provide strong support for knowledge discovery and downstream applications such as intelligent Q&A and intelligent recommendation in the field of wheat sharp eyespot control.
Quality of Life in Patients with Coronary Artery Disease—Multicenter POLASPIRE II Study
Authors: Dąbek Józefa , Styczkiewicz Marek , Kamiński Karol , Kubica Aldona , Kosior Dariusz A. , Wolfshaut-Wolak Renata , Rajzer Marek , Szynal Magdalena , Jankowski Piotr , Gąsior Zbigniew
Published: June 2024

Background: The quality of life of patients with coronary heart disease is extremely important for their treatment. The aim of the study was to assess the quality of life of patients with coronary artery disease, considering education and compliance with medical recommendations regarding lifestyle changes, as well as the presence of selected cardiovascular risk factors. Methods: The study involved 763 patients from 11 Polish cardiology centers. The presented material is part of the multicenter POLASPIRE II study. Patients completed a standardized questionnaire EuroQol 5D-5Lm. A medical interview was conducted with each patient. All patients had their body weight and height measured and BMI determined. Results: The quality of life of patients was better in men, younger people, those with lower body weight and those who followed preventive recommendations and intensified their physical activity. Most of the examined patients complied with the medical recommendations regarding lifestyle changes after a cardiac incident, but they mainly concerned dietary modifications. There was still a large group of patients who did not comply with the recommendations, e.g., regarding increasing physical activity. Conclusions: The assessment of quality of life depended on many factors, such as gender, body weight and compliance with medical recommendations. The health education of patients in the presented study group was not sufficient. Therefore, there is a need for better education regarding the benefits of following medical recommendations in terms of leading a healthy lifestyle, which consequently improves its quality and duration.
Ovarian Tissue Cryopreservation for Fertility Preservation in Patients with Hemoglobin Disorders: A Comprehensive Review
Authors: Haering Catherine , Coyne Kathryn , Daunov Katherine , Anim Samuel , Christianson Mindy S. , Flyckt Rebecca
Published: June 2024

Hemoglobin diseases like sickle cell disease (SCD) and β-thalassemia (BT) present fertility challenges for affected patients. SCD and BT result from abnormal hemoglobin production or structure and pose numerous health concerns. Despite medical advancements improving the quality of life or even providing cures, SCD and BT pose unique fertility concerns for women. Young women with these disorders already contend with reduced ovarian reserve and a narrower fertile window, a situation that is compounded by the gonadotoxic effects of treatments like medications, transfusions, stem cell transplants, and gene therapy. While crucial for disease control, these interventions may lead to reproductive health issues, increasing infertility and early menopause risks. Ovarian tissue cryopreservation (OTC) offers potential for future motherhood to women with hemoglobin disorders facing infertility related to curative treatments. OTC involves surgically removing, preparing, and freezing ovarian tissue containing primordial follicles capable of producing mature oocytes, offering advantages over oocyte cryopreservation alone. However, the application of OTC for patients with hemoglobin disorders presents unique challenges, including special health risks, financial barriers, and access to care. This comprehensive literature review delves into the current state of ovarian tissue cryopreservation for fertility preservation in patients with hemoglobin disorders. Empowering patients with informed reproductive choices in the context of their hemoglobin disorders stands as the ultimate goal.
Evolving Referral Patterns and Management Following Implementation of a Multidisciplinary Fibroid Center: A Retrospective Cohort Study
Authors: Musselman Kelsey , Pepin Kristen , Lamparello Nicole A. , Havryliuk Yelena , Schiffman Marc , Fenster Tamatha , Shin Ja Hyun
Published: June 2024

Background/Objectives: Our objective was to evaluate changes in the management of symptomatic fibroids after establishing a multidisciplinary fibroid center with minimally invasive gynecologic surgery (MIGS) and interventional radiology (IR). Methods: A retrospective cohort study was conducted at the fibroid center created in September 2020. Patients were offered same-day consults with both MIGS and IR providers. Data were collected for patients with initial consultations from January to June 2019 (pre-fibroid center) and from January to June 2021 (post-fibroid center). Results: Among 615 patients meeting inclusion criteria, 273 had consultations pre-center and 342 post-center. More patients seen post-center had previously attempted medical management (30.1% vs. 20.2%), with a significant proportion having no prior medical or surgical treatment (53.2% vs. 61.5%). Post-center, there were more MIGS consultations (65.5% vs. 53.1%) and a decrease in general gynecology (GYN) consultations (19.0% vs. 25.6%). More patients sought additional opinions post-center (83.6% vs. 67.0%), particularly with MIGS (58.8% vs. 37.0%). General GYNs referred to MIGS (79.3% vs. 73.1%) and IR specialists (16.0% vs. 13.0%) more often in 2021. In 2021, use of MRI increased (66.5% vs. 52.4%), and more patients underwent uterine artery embolization (UAE) within 1 year of consultation compared to the pre-center period (13.8% vs. 6.9%). Conclusions: Patients with symptomatic fibroids often seek the expertise of specialists to explore treatment options. A multidisciplinary fibroid center that integrates efforts of MIGS and IR enables thorough counseling and a rise in the utilization of minimally invasive procedures, including UAE.
Ultrasound Shear Wave Elastography Evaluation of the Liver and Implications for Perioperative Medicine
Authors: Suffredini Giancarlo , Gao Wei Dong , Dodd-o Jeffrey M.
Published: June 2024

Ultrasound shear wave elastography (SWE) is a non-invasive, low risk technology allowing the assessment of tissue stiffness. Used clinically for nearly two decades to diagnose and stage liver fibrosis and cirrhosis, it has recently been appreciated for its ability to differentiate between more subtle forms of liver dysfunction. In this review, we will discuss the principle of ultrasound shear wave elastography, its traditional utilization in grading liver cirrhosis, as well as its evolving role in identifying more subtle degrees of liver injury. Finally, we will show how this capacity to distinguish nuanced changes may provide an opportunity for its use in perioperative risk stratification.
Evaluation of Various Methods of Liver Measurement in Comparison to Volumetric Segmentation Based on Computed Tomography
Authors: Cebula Maciej , Biernacka Angelika , Bożek Oskar , Kokoszka Bartosz , Kazibut Sylwia , Kujszczyk Anna , Kulig-Kulesza Monika , Modlińska Sandra , Kufel Jakub , Azierski Michał , Szydło Filip , Winder Mateusz , Pilch-Kowalczyk Joanna , Gruszczyńska Katarzyna
Published: June 2024

Background: A reliable assessment of liver volume, necessary before transplantation, remains a challenge. Our work aimed to assess the differences in the evaluation and measurements of the liver between independent observers and compare different formulas calculating its volume in relation to volumetric segmentation. Methods: Eight researchers measured standard liver dimensions based on 105 abdominal computed tomography (CT) scans. Based on the results obtained, the volume of the liver was calculated using twelve different methods. An independent observer performed a volumetric segmentation of the livers based on the same CT examinations. Results: Significant differences were found between the formulas and in relation to volumetric segmentation, with the closest results obtained for the Heinemann et al. method. The measurements of individual observers differed significantly from one another. The observers also rated different numbers of livers as enlarged. Conclusions: Due to significant differences, despite its time-consuming nature, the use of volumetric liver segmentation in the daily assessment of liver volume seems to be the most accurate method.
Prognostic Impact of Left Ventricular Ejection Fraction Improvement after Transcatheter Aortic Valve Replacement
Authors: Reichl Jakob Johannes , Stolte Thorald , Tang Shihui , Boeddinghaus Jasper , Wagener Max , Leibundgut Gregor , Kaiser Christoph Ado , Nestelberger Thomas
Published: June 2024

Introduction: Transcatheter aortic valve replacement (TAVR) has become an efficient and safe alternative to surgical aortic valve replacement (SAVR). While severe aortic stenosis as well as severe aortic regurgitation (AR) are known to negatively impact left ventricular ejection fraction (LVEF), prior studies have shown that TAVR can lead to an improvement in LVEF. Thus far, little is known about the prognostic implication of LVEF improvement as a sole predictor of outcomes. Therefore, the aim of this study was to assess the prognostic impact of LVEF impairment before TAVR, as well as early LVEF improvement in patients undergoing TAVR. Materials and Methods: Patients undergoing TAVR in a large tertiary university hospital were consecutively included in a prospective registry. Transthoracic echocardiography (TTE) was performed at baseline, after 1 month and annually thereafter. Significant LVEF improvement was defined as a relative increase of ≥10% in LVEF at 30 days compared to baseline LVEF. The primary outcome was all-cause mortality at 1 year. Secondary outcomes were major adverse cardiovascular events (MACEs) including cardiovascular death, non-fatal myocardial infarction, stroke, bleeding and unplanned re-interventions of the aortic valve at 5 years. Results: Among 1655 patients who underwent TAVR between September 2011 and April 2024, the LVEF at baseline was available for 1556 patients. Of these, 1031 patients (66.2%) had preserved LVEF at baseline (LVEF ≥ 53%), whereas 303 patients (19.5%) had moderately reduced LVEF (40–52%) and 222 patients (14.3%) had severely reduced LVEF (<40%). Out of the patients with impaired LVEF, 155 (40.4%) patients showed a significant improvement in LVEF ≥10% after 30 days, while 229 (60.6%) patients showed no significant LVEF improvement (<10%). Patients with preserved LVEF at baseline had significantly better mortality outcomes than those with severely reduced LVEF (p < 0.001). LVEF improvement was associated with a survival benefit after 1 year (p = 0.009, HR 2.68, 0.95 CI 1.23–5.85) which diminished after 5 years (p = 0.058), but patients with LVEF improvement showed lower MACE rates at 5 years (p < 0.001). Conclusions: Preserved LVEF before TAVR is an independent predictor for improved outcomes. Additionally, early improvement in LVEF is associated with beneficial outcomes in patients undergoing TAVR.
Preliminary Evaluation of Bioactive Collagen–Polyphenol Surface Nanolayers on Titanium Implants: An X-ray Photoelectron Spectroscopy and Bone Implant Study
Authors: Morra Marco , Iviglia Giorgio , Cassinelli Clara , Sartori Maria , Cavazza Luca , Martini Lucia , Fini Milena , Giavaresi Gianluca
Published: June 2024

To endow an implant surface with enhanced properties to ensure an appropriate seal with the host tissue for inflammation/infection resistance, next-generation bone implant collagen–polyphenol nanolayers were built on conventional titanium surfaces through a multilayer approach. X-ray Photoelectron Spectroscopy (XPS) analysis was performed to investigate the chemical arrangement of molecules within the surface layer and to provide an estimate of their thickness. A short-term (2 and 4 weeks) in vivo test of bone implants in a healthy rabbit model was performed to check possible side effects of the soft surface layer on early phases of osteointegration, leading to secondary stability. Results show the building up of the different nanolayers on top of titanium, resulting in a final composite collagen–polyphenol surface and a layer thickness of about 10 nm. In vivo tests performed on machined and state-of-the-art microrough titanium implants do not show significant differences between coated and uncoated samples, as the surface microroughness remains the main driver of bone-to-implant contact. These results confirm that the surface nanolayer does not interfere with the onset and progression of implant osteointegration and prompt the green light for specific investigations of the potential merits of this bioactive coating as an enhancer of the device/tissue seal.
Chatter Mitigation in Turning Slender Components Using Viscous Fluids
Authors: Griskevicius Matas , Kharka Vishal , Kilic Zekai Murat
Published: June 2024

This paper investigates the performance of a novel viscous passive damping solution to mitigate the chatter vibrations issue in the context of turning thin-walled cylindrical shell components for aerospace and other industries. This study involves the use of two different viscous fluids, motor oil and silicone oil, which have viscosities of 102 cSt and 350 cSt, respectively, to fill the in-house developed tube components with the aim of improving machining performance. Fast Fourier Transform (FFT) graphs were studied for chatter analysis, and surface roughness parameters such as average surface roughness (Ra) and mean roughness depth (Rz) were considered for studying the effectiveness of the viscous damping fluids. The results obtained with viscous damping were then compared with an undamped/unfilled tube with the same geometry. The cutting experiments showed that the motor oil reduced the excessive vibrations while silicone oil was able to eliminate them. For the tube with motor oil, the magnitude of the process sound at chatter frequency was reduced by 6.6 times as compared to an unfilled tube, whereas for the tube with silicone oil, the amplitude at chatter frequency was reduced by 14.8 times. Moreover, the surface quality of the tubes with motor oil and silicone oil shows almost equal improvement, indicating the need for future research on the type and amount of viscous fluids for implementing the concept in real cases.
Television Debates as a TV Typology: Continuities and Changes in Televised Political Competition—The Case of the 2023 Pre-Election Debates in Greece
Authors: Bourchas Panagiotis Vasileios , Gioltzidou Georgia
Published: June 2024

In the USA, for the first time in the 1960s, and in a very systematic manner from 1976 onwards, pre-election debates (televised presidential debates) have become a fundamental and integral method of communication for political parties, as well as an institution of American democracy that contributes significantly to shaping a culture of public political dialogue at a relatively high level, through which citizens accumulate knowledge about political figures and their parties’ positions within a very short period of time before the elections. In Greece, on the contrary, these television programs have not sparked significant interest to date. The subject of this study is the television debates in Greece, evaluated through a brief historical overview and commentary on their structure, culminating in the two televised confrontations that took place within a five-month period during two electoral contests in 2023. Firstly, the reactions to and reception of the two televised debates by citizens on platform X and, secondly, the commentary on the two debates by journalists, columnists, and renowned analysts, reveal the differing interests of both sides. The research results confirm that, in addition to the performance of politicians, citizens are also interested in the conditions and form in which these pre-election televised debates are staged.
Calcium-Free Dialysate Hemodialysis: A Simplified Approach
Authors: Corbu Alexandra , Terrec Florian , Malvezzi Paolo , Jouzier Arnaud , Jouve Thomas , Rostaing Lionel , Naciri Bennani Hamza
Published: June 2024

Intermittent hemodialysis (HD) in high-bleeding-risk patients presents a challenge as circuit anticoagulation using heparin is contraindicated in such cases. Recently, the use of calcium-free citrate-containing dialysate with calcium supplementation emerged as a viable alternative to heparin-circuit anticoagulation. This is a retrospective, monocentric study to evaluate dialysis efficacy using calcium-free citrate-containing dialysate with calcium reinjection into the venous line in hemodialysis patients at risk of bleeding. A total of 53 patients were analyzed: 52 had a temporary contraindication to systemic anticoagulation (active bleeding or surgical intervention), and 1 chronic HD patient had prolonged bleeding time due to inoperable arteriovenous fistula stenosis. Only 7 out of 79 dialysis sessions performed were prematurely terminated (vascular access dysfunction). The median dialysis time was 240 min (range: 150–300). The chronic dialysis patient had 108 sessions with no premature termination. Frequent monitoring of ionized calcium was performed throughout the dialysis sessions: levels remained stable at T0 and T + 60 min (1.08 ± 0.08 mmol/L) and slightly increased at the end of the dialysis session (1.19 ± 0.13 mmol/L), remaining within normal limits. Target postfilter ionized calcium <0.4 mmol/L was achieved in all sessions (0.31 ± 0.07 mmol/L). There were no cases of symptomatic hypo-/hypercalcemia and no need for calcium infusion rate adjustment throughout the sessions. Hemodialysis with calcium-free citrate-containing dialysate and calcium reinjection into the venous line is efficient and safe in HD patients with contraindications to systemic anticoagulation.
Repetitive Transcranial Magnetic Stimulation in Fibromyalgia: Exploring the Necessity of Neuronavigation for Targeting New Brain Regions
Authors: Velickovic Zoran , Radunovic Goran
Published: June 2024

Fibromyalgia and osteoarthritis are among the most prevalent rheumatic conditions worldwide. Nonpharmacological interventions have gained scientific endorsements as the preferred initial treatments before resorting to pharmacological modalities. Repetitive transcranial magnetic stimulation (rTMS) is among the most widely researched neuromodulation techniques, though it has not yet been officially recommended for fibromyalgia. This review aims to summarize the current evidence supporting rTMS for treating various fibromyalgia symptoms. Recent findings: High-frequency rTMS directed at the primary motor cortex (M1) has the strongest support in the literature for reducing pain intensity, with new research examining its long-term effectiveness. Nonetheless, some individuals may not respond to M1-targeted rTMS, and symptoms beyond pain can be prominent. Ongoing research aims to improve the efficacy of rTMS by exploring new brain targets, using innovative stimulation parameters, incorporating neuronavigation, and better identifying patients likely to benefit from this treatment. Summary: Noninvasive brain stimulation with rTMS over M1 is a well-tolerated treatment that can improve chronic pain and overall quality of life in fibromyalgia patients. However, the data are highly heterogeneous, with a limited level of evidence, posing a significant challenge to the inclusion of rTMS in official treatment guidelines. Research is ongoing to enhance its effectiveness, with future perspectives exploring its impact by targeting additional areas of the brain such as the medial prefrontal cortex, anterior cingulate cortex, and inferior parietal lobe, as well as selecting the right patients who could benefit from this treatment.
The Evolving Role of Bispecific Antibodies in Diffuse Large B-Cell Lymphoma
Authors: Saleh Khalil , Khoury Rita , Khalife Nadine , Chahine Claude , Ibrahim Rebecca , Tikriti Zamzam , Le Cesne Axel
Published: June 2024

The advent of targeted therapies such as monoclonal antibodies, adoptive T-cell therapies, and antibody–drug conjugates (ADCs) dramatically changed the treatment landscape of diffuse large B-cell lymphoma (DLBCL) over the last two decades. Rituximab was the first one approved. Chimeric antigen receptor T-cells are currently approved as second-line treatment in patients with DLBCL refractory to first-line chemo-immunotherapy. Polatuzumab, a CD79b-targeting ADC, is approved as first-line treatment in high-risk patients in combination with chemo-immunotherapy. Bispecific antibodies (BsAbs) are a novel category of drugs that are also changing the treatment paradigm of patients with DLBCL. They are engineered to bind to two different targets at the same time. To date, two BsAbs (glofitamab and epcoritamab) are approved as monotherapy in third-line treatment in DLBCL. Combination strategies with chemotherapy, immunotherapy, and ADCs are currently under investigation with encouraging results in first-line or subsequent lines of treatment. In the following review, we focus on the structure of BsAbs, the mechanism of action, clinical efficacy, and the mechanisms of resistance to BsAbs.
Association of Coronavirus Disease 2019 Vaccination with Facial-Related Neurological Disorders: A Nationwide Retrospective Cohort Study
Authors: Kim Younggoo , Kim Min-Ho , Chun Eunmi , Cho Dosang
Published: June 2024

Neurological complications after the coronavirus disease 2019 (COVID-19) vaccine administration have been reported. However, the incidence rates of these complications have not been compared in vaccinated and unvaccinated individuals. This study used a nationwide cohort from South Korea to investigate the incidence and prognostic factors of facial-related neurological disorders, such as facial palsy, trigeminal neuralgia, and hemifacial spasms, after COVID-19 vaccination. A population-based cohort design was used to examine data from a randomly selected 50% of the adult population in Seoul, South Korea. Information on demographics, vaccination status, vaccination type, and medical history was collected. The incidence rates and adjusted hazard ratios (aHRs) for facial-related neurological disorders were calculated. This study included 2,482,481 adults, 85.94% of whom were vaccinated. Vaccinated individuals showed a higher incidence of facial palsy, hemifacial spasm, and trigeminal neuralgia than unvaccinated individuals, with significant aHRs of 1.821, 3.203, and 6.621, respectively. Dyslipidemia, female sex, and young age were identified as risk factors for hemifacial spasms and trigeminal neuralgia. This study demonstrates an increased incidence of facial-related neurological disorders after COVID-19 vaccination, particularly among individuals with dyslipidemia and younger women. These findings underscore the need for further investigations into the mechanisms and management of vaccine-related neurological issues.
Financial Risk Management Early-Warning Model for Chinese Enterprises
Authors: Wei Haitong , Wang Xinghai
Published: June 2024

As enterprises face increasing competitive pressures, financial crises can significantly impact on their capital operations, potentially leading to operational difficulties and, ultimately, market exclusion. Consequently, many enterprises have begun to utilize financial early-warning systems to guide and control risks. Currently, there is neither a universal nor comprehensive enterprise financial risk management model in China, nor a unified classification standard for enterprise financial risk management levels. This article takes financial data on A-share listed companies in 2020 as the data sample, including those with special treatment (represented by ST) or non-ST status. We establish an independent indicator system within the framework of profitability, solvency, operational capability, development potential, shareholders’ retained earnings, cash flow, and asset growth. The model is constructed employing the factor–logistic fusion algorithm. The factor part addresses the issue of collinearity among risk indicators, and the logistic part presents the results in probabilistic form, enhancing the interpretability of the model. The prediction accuracy of this model exceeds 89%. Finally, by applying the principles of interval estimation theory to statistical hypothesis testing, we categorize the risk levels into Grade A, representing significant risk; Grade B, representing moderate risk; Grade C, representing minor risk; and Grade D, representing no risk. This article aims to provide a comprehensive definition of a universal financial risk management early-warning model applicable to all enterprises in China.
Estimation of Vehicle Traffic Parameters Using an Optical Distance Sensor for Use in Smart City Road Infrastructure
Authors: Burdzik Rafał , Celiński Ireneusz , Ragulskis Minvydas , Ranjan Vinayak , Matijošius Jonas
Published: June 2024

In recent decades, the dynamics of road vehicle traffic have significantly evolved, compelling traffic engineers to develop innovative traffic monitoring solutions, especially for dense road networks. Traditional methods for measuring traffic volume along road sections may no longer suffice for modern traffic control systems. This is particularly true for induction loops, a widely used method since the last century. In contrast, measuring techniques using microwaves or visible light offer better accuracy but are often hindered by the high cost of sensors. This paper presents new techniques for measuring traffic flow and other parameters that adapt to changing traffic dynamics using low-cost optical distance sensors. Our study demonstrates that the integration of multiple monitoring approaches enhances measurement accuracy, contingent on the dynamics and specific characteristics of the traffic. The results indicate that cheap optical distance sensors are particularly well suited for use in smart city road networks.
Blockchain and Supply-Chain Financing: An Evolutionary Game Approach with Guarantee Considerations
Authors: Zhan Jizhou , Zhang Gewei , Chong Heap-Yih , Chen Xiangfeng
Published: June 2024

Blockchain technology enables innovative financing models in supply-chain finance. This research constructs a tripartite evolutionary game model that includes core enterprises as employers, small- and medium-sized enterprises (SMEs) as contractors, and banks as financial institutions, where they have been simulated for their impact on blockchain technology, especially on the strategic choices of supply-chain financing behavior and the system’s evolutionary path under core enterprises’ guarantee mechanism. The findings show the application of blockchain technology can effectively reduce the regulatory and review costs for financial institutions, thereby enhancing the efficiency of supply-chain financing. Particularly, blockchain technology provides a more reliable credit endorsement platform for SMEs in reducing their tendency to default. The guarantee mechanism of core enterprises is more effective with the support of blockchain technology, which helps to build more solid supply-chain financial cooperation relationships. The research contributes to the theoretical research on the integration of blockchain technology into supply-chain finance, especially for improving the operational efficiency of financial services. It also highlights the need for blockchain-backed guarantees from core enterprises in optimizing supply-chain financial services.
Insights into the Regulation of the Mitochondrial Inheritance and Trafficking Adaptor Protein Mmr1 in Saccharomyces cerevisiae
Authors: Nayef Nourah , Ekal Lakhan , Hettema Ewald H. , Ayscough Kathryn R.
Published: June 2024

Mitochondria are organelles involved in cellular energetics in all eukaryotes, and changes in their dynamics, fission, fusion, or localization can lead to cell defects and disease in humans. Budding yeast, Saccharomyces cerevisiae, has been shown to be an effective model organism in elucidating mechanisms underpinning these mitochondrial processes. In the work presented here, a genetic screen was performed to identify overexpressing kinases, phosphatases, and ubiquitin ligases, which resulted in mitochondrial defects. A total of 33 overexpressed genes showed mitochondrial phenotypes but without severe growth defects. These included a subset that affected the timing of mitochondrial inheritance and were the focus of further study. Using cell and biochemical approaches, the roles of the PAK-family kinase Cla4 and the E3-ubiquitin ligases Dma1 and Dma2 were investigated. Previous studies have indicated the roles of kinase Cla4 and ligases Dma1 and Dma2 in triggering the degradation of trafficking adaptors in the bud, which leads to disruption of the interaction with the transporting class V myosin, Myo2. Here, we map a key interface between Cla4 and the mitochondrial adaptor Mmr1 necessary for phosphorylation and identify a region of Mmr1 required for its degradation via Dma1 and Dma2. Together, our data provide insights into key regulatory regions of Mmr1 responsible for its function in mitochondrial inheritance.
Protein Phosphorylation Nexus of Cyanobacterial Adaptation and Metabolism
Authors: Nawaz Taufiq , Fahad Shah , Zhou Ruanbao
Published: June 2024

Protein phosphorylation serves as a fundamental regulatory mechanism to modulate cellular responses to environmental stimuli and plays a crucial role in orchestrating adaptation and metabolic homeostasis in various diverse organisms. In cyanobacteria, an ancient phylum of significant ecological and biotechnological relevance, protein phosphorylation emerges as a central regulatory axis mediating adaptive responses that are essential for survival and growth. This exhaustive review thoroughly explores the complex terrain of protein phosphorylation in cyanobacterial adaptation and metabolism, illustrating its diverse forms and functional implications. Commencing with an overview of cyanobacterial physiology and the historical trajectory of protein phosphorylation research in prokaryotes, this review navigates through the complex mechanisms of two-component sensory systems and their interplay with protein phosphorylation. Furthermore, it investigates the different feeding modes of cyanobacteria and highlights the complex interplay between photoautotrophy, environmental variables, and susceptibility to photo-inhibition. The significant elucidation of the regulatory role of protein phosphorylation in coordinating light harvesting with the acquisition of inorganic nutrients underscores its fundamental importance in the cyanobacterial physiology. This review highlights its novelty by synthesizing existing knowledge and proposing future research trajectories, thereby contributing to the deeper elucidation of cyanobacterial adaptation and metabolic regulation through protein phosphorylation.
The Impact of Projected Land Use Changes on the Availability of Ecosystem Services in the Upper Flint River Watershed, USA
Authors: Abbasnezhad Behnoosh , Abrams Jesse B. , Wenger Seth J.
Published: June 2024

The conversion of forestlands to alternative land uses is a growing worldwide concern, given the wide range of provisioning and regulating ecosystem services (ES) provided by forests. We applied a scenario-based land-use/land-cover (LULC) projection technique integrating societal preferences, conservation policies, and socio-economic factors to the Upper Flint River Watershed in the Atlanta, Georgia (USA) metropolitan area. We employed the InVEST modeling toolset to assess the impact of anticipated LULC changes on ES under each development scenario. Our simulations projected a consistent conversion from Deciduous/Mixed Forests to either Urban or Evergreen forests across all scenarios, leading to a significant decline in ES. We quantified the economic impacts of this ES loss, conservatively estimated as representing millions of dollars per year under a Business as Usual scenario in just carbon and water services alone. Integrating social and policy drivers into our projection approach yielded policy-relevant results and identified the need for conservation policy instruments to protect forested ecosystems with higher conservation values. Existing conservation policies are unlikely to stem the loss of important ES, and there may be a need to consider more aggressive policies to prevent further degradation of watersheds, such as the one analyzed here.
The Effectiveness of Administrative Power on Farmers’ Attitude in Land Acquisition Negotiation: Mediating Role of Perceived Fairness
Authors: Cheng Feiyu , Liu Yisheng , Liu Boyang
Published: June 2024

Farmers’ attitude plays a crucial role in the success of land acquisition. To add new insights and theoretical support for the effective governance of land acquisition, this paper examines the effectiveness of administrative power on farmers’ attitude and the mediating role of perceived fairness based on asymmetric dependency theory. The hypotheses were verified using Structural Equation Modeling (SEM) analysis conducted with data gathered from 250 questionnaire samples in rural areas of China. The results reveal that the coercion and transparency of administrative power have a significant positive impact on farmers’ attitude while normality’s influence is not significant. The coercion and transparency of administrative power have a significant positive impact on fairness while normality’s impact is not significant. The mediating effect of perceived fairness was insignificant. Therefore, local governments could encourage farmers to embrace a cooperative attitude in land acquisition negotiations by fostering a constructive interaction between the government and the public and pursuing a high-level institutional balance driven by a service-oriented government.
Comprehensive Land Consolidation as a Development Strategy for Rural Revitalization: The Political Ecology Mechanisms and Benefits of the Pastoral Complex
Authors: Chen Borui , Huang Zirou , He Wei , Wang Min
Published: June 2024

Rapid urbanization in China has shifted the logic and needs of rural resource allocation, prompting a transformative approach toward rural revitalization and sustainable development through comprehensive land consolidation. This paper offers a theoretical framework for understanding the driven adjustments in rural power, capital, and social structures facilitated by comprehensive land consolidation. It discusses how the construction of pastoral complexes influences the diversification of rural land use and ecological restoration through dynamic adjustments in power structures. This study illustrates that in Guangzhou, comprehensive land consolidation is indispensable for ensuring food security and fostering diverse rural industries. Furthermore, it facilitates the resource and commercialization of land, balances governmental social responsibilities with rational capital pursuits, and enriches the livelihood structures of social entities to ensure rural social equity. The pastoral complex model achieves a harmonious integration of rural socio-economic and natural systems. Innovations in paths of social participation help eliminate the effects of social inequality within the environment, while the theory of political ecology clearly analyzes the internal reasons for the interaction and strategic plays among diverse actors under this model. The case studies of the pastoral complex present the practice of national–capital–land–social relationships and changes in land benefits, showcasing a Chinese approach to rural development and social justice. This provides a new perspective for political ecology research and enriches its empirical content.