bluuebunny/crossref_metadata_2025 huggingface dataset:
Forecasting subnational COVID‐19 mortality using a day‐of‐the‐week adjusted Bayesian hierarchical model
Authors: Slater Justin J. , Brown Patrick E. , Rosenthal Jeffrey S.
Published: November 2020

As of October 2020, the death toll from the COVID‐19 pandemic has risen over 1.1 million deaths worldwide. Reliable estimates of mortality due to COVID‐19 are important to guide intervention strategies such as lockdowns and social distancing measures. In this paper, we develop a data‐driven model that accurately and consistently estimates COVID‐19 mortality at the regional level early in the epidemic, using only daily mortality counts as the input. We use a Bayesian hierarchical skew‐normal model with day‐of‐the‐week parameters to provide accurate projections of COVID‐19 mortality. We validate our projections by comparing our model to the projections made by the Institute for Health Metrics and Evaluation and highlight the importance of hierarchicalization and day‐of‐the‐week effect estimation.
Geography of suicide in Japan: spatial patterning and rural–urban differences
Authors: Yoshioka Eiji , Hanley Sharon J. B. , Sato Yukihiro , Saijo Yasuaki
Published: November 2020

Purpose

There are notable geographic variations in incidence rates of suicide both in Japan and globally. Previous studies have found that rurality/urbanity shapes intra-regional differences in suicide mortality, and suicide risk associated with rurality can vary significantly by gender and age. This study aimed to examine spatial patterning of and rural–urban differences in suicide mortality by gender and age group across 1887 municipalities in Japan between 2009 and 2017.

Methods

Suicide data were obtained from suicide statistics of the Ministry of Health, Labour and Welfare in Japan. We estimated smoothed standardized mortality ratios for suicide for each of the municipalities and investigated associations with level of rurality/urbanity using Bayesian hierarchical models before and after adjusting for socioeconomic characteristics.

Results

The results of the multivariate analyses showed that, for males aged 0–39 and 40–59 years, rural residents tended to have a higher suicide risk compared to urban ones. For males aged 60+ years, a distinct rural–urban gradient in suicide risk was not observed. For females aged 0–39 years, a significant association between suicide risk and rurality was not observed, while for females aged 40–59 years and females aged 60 years or above, the association was a U-shaped curve.

Conclusion

Our results showed that geographical distribution of and rural–urban differences in suicide mortality in Japan differed substantially by gender and age. These findings suggest that it is important to take demographic factors into consideration when municipalities allocate resources for suicide prevention.
Concurrent optimization of dimensions and tolerances on structures and mechanisms
Authors: Armillotta Antonio
Published: November 2020

The paper deals with a problem of robust optimization of mechanical assemblies, which combines the allocation of tolerances with the selection of dimensional parameters. The two tasks are carried out together with the aim of minimizing the manufacturing cost and the variation on an assembly-level functional characteristic. The problem is addressed in the specific context of planar linkages used in structures and mechanisms. The proposed solution is based on an optimality condition involving both tolerances and dimensions, which allows to define a joint optimization problem avoiding the need of two sequential optimization phases. The condition is developed with the method of Lagrange multipliers using an expanded formulation of the reciprocal power cost-tolerance function. The optimal tolerances depend on the stackup coefficients of the output characteristic, which are calculated with a tolerance analysis method based on a static analogy. The procedure is demonstrated on two examples to illustrate some application details and discuss potential advantages and limitations.
Microbial Key Players Involved in P Turnover Differ in Artificial Soil Mixtures Depending on Clay Mineral Composition
Authors: Tanuwidjaja Irina , Vogel Cordula , Pronk Geertje J. , Schöler Anne , Kublik Susanne , Vestergaard Gisle , Kögel-Knabner Ingrid , Mrkonjic Fuka Mirna , Schloter Michael , Schulz Stefanie
Published: November 2020

Nutrient turnover in soils is strongly driven by soil properties, including clay mineral composition. One main nutrient is phosphorus (P), which is known to be easily immobilized in soil. Therefore, the specific surface characteristics of clay minerals might substantially influence P availability in soil and thus the microbial strategies for accessing P pools. We used a metagenomic approach to analyze the microbial potential to access P after 842 days of incubation in artificial soils with a clay mineral composition of either non-expandable illite (IL) or expandable montmorillonite (MT), which differ in their surface characteristics like soil surface area and surface charge. Our data indicate that microorganisms of the two soils developed different strategies to overcome P depletion, resulting in similar total P concentrations. Genes predicted to encode inorganic pyrophosphatase (ppa), exopolyphosphatase (ppx), and the pstSCAB transport system were higher in MT, suggesting effective P uptake and the use of internal poly-P stores. Genes predicted to encode enzymes involved in organic P turnover like alkaline phosphatases (phoA, phoD) and glycerophosphoryl diester phosphodiesterase were detected in both soils in comparable numbers. In addition, Po concentrations did not differ significantly. Most identified genes were assigned to microbial lineages generally abundant in agricultural fields, but some were assigned to lineages known to include oligotrophic specialists, such as Bacillaceae and Microchaetaceae.
The effect of bile acids on the growth and global gene expression profiles in Akkermansia muciniphila
Authors: Hagi Tatsuro , Geerlings Sharon Y. , Nijsse Bart , Belzer Clara
Published: November 2020

Akkermansia muciniphila is a prominent member of the gut microbiota and the organism gets exposed to bile acids within this niche. Several gut bacteria have bile response genes to metabolize bile acids or an ability to change their membrane structure to prevent membrane damage from bile acids. To understand the response to bile acids and how A. muciniphila can persist in the gut, we studied the effect of bile acids and individual bile salts on growth. In addition, the change in gene expression under ox-bile condition was studied. The growth of A. muciniphila was inhibited by ox-bile and the bile salts mixture. Individual bile salts have differential effects on the growth. Although most bile salts inhibited the growth of A. muciniphila, an increased growth was observed under culture conditions with sodium deoxycholate. Zaragozic acid A, which is a squalene synthase inhibitor leading to changes in the membrane structure, increased the susceptibility of A. muciniphila to bile acids. Transcriptome analysis showed that gene clusters associated with an ABC transporter and RND transporter were upregulated in the presence of ox-bile. In contrast, a gene cluster containing a potassium transporter was downregulated. Membrane transporter inhibitors also decreased the tolerance to bile acids of A. muciniphila. Our results indicated that membrane transporters and the squalene-associated membrane structure could be major bile response systems required for bile tolerance in A. muciniphila.

Key points

• The growth of Akkermansia muciniphila was inhibited by most bile salts.

• Sodium deoxycholate increased the growth of A. muciniphila.

• The genes encoding transporters and hopanoid synthesis were upregulated by ox-bile.

• The inhibitors of transporters and hopanoid synthesis reduced ox-bile tolerance.
Development of a double monoclonal antibody–based sandwich enzyme-linked immunosorbent assay for detecting canine distemper virus
Authors: Zhang Yuan , Xu Gang , Zhang Lu , Zhao Jiakai , Ji Pinpin , Li Yaning , Liu Baoyuan , Zhang Jingfei , Zhao Qin , Sun Yani , Zhou En-Min
Published: November 2020

Canine distemper virus (CDV) infection causes mass mortality in diverse carnivore species. For effective virus surveillance, rapid and sensitive assays are needed to detect CDV in field samples. In this study, after BABL/c mice were immunized with recombinant CDV-fusion (F) protein, monoclonal antibodies (mAbs) against recombinant CDV-F protein (designated 1A5, 1A6, and 7D5) were produced using traditional hybridoma cell technology. Next, capture antibody (1A6, 800 ng/well) and horseradish peroxidase (HRP)–conjugated detection antibody (HRP-7D5, 1:100, 500 ng/well) were used in a double monoclonal antibody–based sandwich enzyme-linked immunosorbent assay (ELISA) for CDV detection after optimization of both mAb amounts per well using a checkerboard titration test. Based on sandwich ELISA test results for 120 known CDV-negative samples, the cutoff value for a positive result was set to an OD450 nm value ≥ 0.196. As compared with test results obtained from commercial immune colloidal gold test strips, the low limits of detection for the two assays were revealed to be 100 TCID50 per 100 μL. In addition, the sandwich ELISA agreed 100% and 96.4% with commercial immune colloidal gold test strips when testing serum and stool samples. The sandwich ELISA assay provided statistically similar CDV detection. Thus, the sandwich ELISA developed here to detect CDV in fecal and serum samples provided good sensitivity, high specificity, and good reproducibility and should serve as an ideal method for large-scale surveillance of CDV infections in carnivores.

Key points

• Three CDV mAbs that recognized different epitopes and bound to virion were generated.

• The sandwich ELISA based mAbs to detect CDV in fecal and serum samples was developed.

• The sandwich ELISA is an ideal method for detecting CDV infections in the field.
“What are you going to do, Protest the Wind?”: Community Perceptions of Emergent and Worsening Coastal Erosion from the Remote Bering Sea Community of St. Paul, Alaska
Authors: Tran Jessica , Divine Lauren M. , Heffner Leanna R.
Published: November 2020

The state of Alaska is experiencing increased coastal erosion due to climatic changes that threaten shoreline, infrastructure, and Alaska Native ways of life. While several Alaska Native villages have been impacted by severe erosion, additional communities face burgeoning erosion concerns. St. Paul, a remote island located in the Bering Sea, Alaska, and home to ~450 Unangan, or Aleut, residents, is experiencing relatively new erosion and associated flooding issues. This study aimed to inform St. Paul’s erosion monitoring and climate adaptation strategies by documenting community perceptions of coastal erosion as an ecological and social threat within a broader context of multiple established climate stressors. We interviewed 21 residents to answer: (1) what are the community’s perceptions of erosion on St. Paul in the context of the island’s other environmental concerns?; (2) do current perceptions of erosion affect how local governing and management entities address erosion impacts?; and (3) how does erosion relate to and impact Unangan cultural traditions and heritage? Residents identified six locations of primary concern, owing to how erosion of those areas impact their culture, subsistence practices, and sense of place. We suggest methods in which local entities can better support proactive climate adaptation and mitigation measures and utilize resources for community-driven adaption planning. By documenting perspectives in Indigenous communities on emergent climate impacts, as well as perceptions of adaptation planning and implementation, it can establish the foundation for more collaborative, culturally relevant, and successful community-driven climate adaptation planning.
Analysis of the impact of adherence to guidelines and expert advice in patients with myelodysplastic syndromes
Authors: Kasprzak A. , Nachtkamp K. , Kondakci M. , Schroeder T. , Kobbe G. , Kündgen A. , Kaivers J. , Rautenberg C. , Haas R. , Gattermann N. , Bonadies N. , Germing U.
Published: November 2020

The European Leukemia Net (ELN) guidelines for treatment of myelodysplastic syndromes (MDS) connect heterogeneous MDS subgroups with a number of therapeutic options ranging from best supportive care to allogeneic stem cell transplantation (alloSCT). However, it is currently unknown whether adherence to guideline recommendations translates into improved survival. The sizeable database of the Duesseldorf MDS Registry allowed us to address this question. We first performed a retrospective analysis including 1698 patients (cohort 1) to whom we retrospectively applied the ELN guidelines. We compared patients treated according to the guidelines with patients who deviated from it, either because they received a certain treatment though it was not recommended or because they did not receive that treatment despite being eligible. We also performed a prospective study with 381 patients (cohort 2) who were seen in our department and received guideline-based expert advice. Again, we compared the impact of subsequent guideline-adherent versus non-adherent treatment. For the majority of treatment options (best supportive care, lenalidomide, hypomethylating agents, low-dose chemotherapy, and intensive chemotherapy), we found that adherence to the ELN guidelines did not improve survival in cohort 1. The same was true when patient management was prospectively enhanced through guideline-based treatment advice given by MDS experts (cohort 2). The only exceptions were alloSCT and iron chelation (ICT). Patients receiving ICT and alloSCT as recommended fared significantly better than those who were eligible but received other treatment. Our analysis underscores the limited survival impact of most MDS therapies and suggests to pursue alloSCT in all suitable candidates.
Acute viral bronchiolitis as a cause of pediatric acute respiratory distress syndrome
Authors: Ghazaly Marwa M. H. , Abu Faddan Nagla H. , Raafat Duaa M. , Mohammed Nagwa A. , Nadel Simon
Published: November 2020

The Pediatric Acute Lung Injury Consensus Conference (PALICC) published pediatric-specific guidelines for the definition, management, and research in pediatric acute respiratory distress syndrome (PARDS). Acute viral bronchiolitis (AVB) remains one of the leading causes of admission to PICU. Respiratory syncytial virus (RSV) is the most common cause of AVB. We aimed to evaluate the incidence of PARDS in AVB and identify the risk of RSV as a trigger pathogen for PARDS. This study is a retrospective single-center observational cohort study including children < 2 years of age admitted to the pediatric intensive care unit at St Mary’s Hospital, London, and presented with AVB in 3 years (2016–2018). Clinical and demographic data was collected; PALICC criteria were applied to define PARDS. Data was expressed as median (IQR range); non-parametric tests were used. In this study, 144 infants with acute viral bronchiolitis were admitted to PICU in the study period. Thirty-nine infants fulfilled criteria of PARDS with RSV as the most common virus identified. Bacterial infection was identified as a risk factor for development of PARDS in infants with AVB.

Conclusion: AVB is an important cause of PARDS in infants. RSV is associated with a higher risk of PARDS in AVB. Bacterial co-infection is a significant risk factor for development of PARDS in AVB.

What is Known:• Bronchiolitis is a common cause of respiratory failure in children under 2 years.• ARDS is a common cause of PICU admission.

What is New:• Evaluation of bronchiolitis as a cause of PARDS according to the PALLIC criteria.• Evaluation of different viruses’ outcome in PARDS especially RSV as a commonest cause of AVB.
Regeneration of the flatworm Prosthiostomum siphunculus (Polycladida, Platyhelminthes)
Authors: Schadt Tamara , Prantl Veronika , Grosbusch Alexandra L , Bertemes Philip , Egger Bernhard
Published: November 2020

Fueled by the discovery of head regeneration in triclads (planarians) two and a half centuries ago, flatworms have been the focus of regeneration research. But not all flatworms can regenerate equally well and to obtain a better picture of the characteristics and evolution of regeneration in flatworms other than planarians, the regeneration capacity and stem cell dynamics during regeneration in the flatworm order Polycladida are studied. Here, we show that as long as the brain remained at least partially intact, the polyclad Prosthiostomum siphunculus was able to regenerate submarginal eyes, cerebral eyes, pharynx, intestine and sucker. In the complete absence of the brain only wound closure was observed but no regeneration of missing organs. Amputated parts of the brain could not be regenerated. The overall regeneration capacity of P. siphunculus is a good fit for category III after a recently established system, in which most polyclads are currently classified. Intact animals showed proliferating cells in front of the brain which is an exception compared with most of the other free-living flatworms that have been observed so far. Proliferating cells could be found within the regeneration blastema, similar to all other flatworm taxa except triclads. No proliferation was observed in epidermis and pharynx. In pulse-chase experiments, the chased cells were found in all regenerated tissues and thereby shown to differentiate and migrate to replace the structures lost upon amputation.
Effect of whole-body vibration on freezing and flexibility in Parkinson’s disease—a pilot study
Authors: Dincher Andrea , Becker Paula , Wydra Georg
Published: November 2020

Background

Parkinson’s disease is the second most common neurodegenerative disease. Symptoms are treated by medication, physio-, exercise, and occupational therapy. Alternative methods have been used in exercise therapy for a few years now. The effect of whole-body vibration as an alternative training method has been investigated for several symptoms in Parkinson’s disease. Since freezing and flexibility have not yet been investigated, the aim of this study was to evaluate the efficacy of different frequencies of application for these two symptoms.

Methods

Patients were randomly assigned to a frequency (6, 12, or 18 Hz) or the control group. Before and after the treatment of 5 × 60 s with a rest of 60 s each, the Sit and Reach test (flexibility) and the 360° turn test (freezing) were performed.

Results

Only the Sit and Reach test showed a significant improvement at 18 Hz (improvement from − 5.75 to − 1.89 cm, F(3,30) = 5.98**). At 360° turn, no significant differences were found. Weak to high effect sizes (standardized mean differences) were determined for the different frequencies, both for the Sit and Reach (from .01 to .64) and for the 360° turn (from − .72 to − 1.25). The highest effect size is observed for 18 Hz and the lowest for 6 Hz.

Conclusions

Higher frequencies seem to be more effective than lower ones. Freezing, age, and gender also seem to play a role. Therefore, this should be investigated in further studies.
Entropic-Based Separation of Diastereomers: Size-Exclusion Chromatography with Online Viscometry and Refractometry Detection for Analysis of Blends of Mannose and Galactose Methyl-α-pyranosides at “Ideal” Size-Exclusion Conditions
Authors: Striegel André M. , Trainoff Steven P.
Published: November 2020

The separation of carbohydrate diastereomers by an ideal size-exclusion mechanism, i.e., in the absence of enthalpic contributions to the separation, can be considered one of the grand challenges in chromatography: Can a difference in the location of a single axial hydroxy group on a pyranose ring (e.g., the axial OH being located on carbon 2 versus on carbon 4 of the ring) sufficiently affect the solution conformational entropy of a monosaccharide in a manner which allows for members of a diastereomeric pair to be separated from each other by size-exclusion chromatography (SEC)? Previous attempts at answering this question, for aqueous solutions, have been thwarted by the mutarotation of sugars in water. Here, the matter is addressed by employing the non-mutarotating methyl-α-pyranosides of d-mannose and d-galactose. We show for the first time, using SEC columns, the entropically driven separation of members of this diastereomeric pair, at a resolution of 1.2–1.3 and with only a 0.4–1% change in solute distribution coefficient over a 25 °C range, thereby demonstrating the ideality of the separation. It is also shown how the newest generation of online viscometer allows for improved sensitivity, thereby extending the range of this so-called molar-mass-sensitive detector into the monomeric regime. Detector multidimensionality is showcased via the synergism of online viscometry and refractometry, which combine to measure the intrinsic viscosity and viscometric radius of the sugars continually across the elution profiles of each diastereomer, methyl-α-d-mannopyranoside and methyl-α-d-galactopyranoside.
Inducible morphological defense in Daphnia pulex: food quantity effects revised
Authors: Klintworth Sandra , von Elert Eric
Published: November 2020

In aquatic systems, organisms largely rely on chemical cues to perceive information about the presence of predators or prey.Daphniarecognize the presence of the predatory larvae ofChaoborusvia a chemical cue, emitted by the larvae, a so-called kairomone. Upon recognition, neckteeth, an alteration of the carapace, are induced inDaphniathat reduce predation rates ofChaoborus. Neckteeth induction was often reported to entail costs. In a previous study, food quantity affected the level of neckteeth induction, with stronger neckteeth induction at low food concentrations and weak induction at high food concentrations. However, reducing neckteeth induction at high food quantities seems to be maladaptive and not in accordance with the concept that inducible defenses are associated with costs.

Here, we hypothesized that weaker neckteeth induction at high food concentrations is caused by increased bacterial degradation of the kairomone. More specifically, we assume that higher algal food concentration is associated with higher bacterial abundances, which degrade the kairomone during the experiment. We tested our hypothesis by treating food algae with antibiotics before providing them as food toDaphnia. Antibiotics reduced bacterial abundances at high and low food concentrations. Reduced bacterial abundances at high food concentrations led to the same level of neckteeth induction as at low food concentrations. A linear regression revealed a significant correlation of neckteeth induction to bacterial abundances. We therefore conclude that differences in neckteeth induction at different food concentrations are not caused by the food quantity effects but by differences in bacterial degradation of the kairomone.
Writing a Novel with Roma Primary School Children: Tensions in Disrupting Aetonormativity
Authors: Dobson Tom , Stephenson Lisa , De Arede Ana
Published: November 2020

Story Makers Press (SMP) is a University-based publisher which co-constructs stories with under-represented groups of children in order to diversify representation in children’s literature and disrupt the way adult perceptions of normality pattern children’s literature (aetonormativity). In this paper we analyse six drama and creative writing workshops run by SMP with Czech and Slovak Roma children from an inner city primary school in the north of England to co-construct a story about climate change. Our analysis identifies how in developing the story, the children were often reluctant to draw upon their funds of knowledge relating to their Roma backgrounds, instead Westernising their protagonists and settings. We also explore how the children disrupt aetonormativity by interweaving magical elements into realistic narrative about climate change in order to establish a genre of magical realism. Finally, we identify how this genre of magical realism is problematic when considering stereotypical depictions of Roma characters in children’s literature and how changes were made to our story in light of a critical race theory reading of the first draft. As well as helping SMP to refine its processes, this analysis suggests that minority groups such as Roma need to be able to draw upon more literary representations of Roma in order to shape their creative outputs and that the curriculum needs to focus on developing children’s critical responses to the representation of minority ethnic groups in children’s literature.
One Instructional Sequence Fits all? A Conceptual Analysis of the Applicability of Concreteness Fading in Mathematics, Physics, Chemistry, and Biology Education
Authors: Kokkonen Tommi , Schalk Lennart
Published: November 2020

To help students acquire mathematics and science knowledge and competencies, educators typically use multiple external representations (MERs). There has been considerable interest in examining ways to present, sequence, and combine MERs. One prominent approach is the concreteness fading sequence, which posits that instruction should start with concrete representations and progress stepwise to representations that are more idealized. Various researchers have suggested that concreteness fading is a broadly applicable instructional approach. In this theoretical paper, we conceptually analyze examples of concreteness fading in the domains of mathematics, physics, chemistry, and biology and discuss its generalizability. We frame the analysis by defining and describing MERs and their use in educational settings. Then, we draw from theories of analogical and relational reasoning to scrutinize the possible cognitive processes related to learning with MERs. Our analysis suggests that concreteness fading may not be as generalizable as has been suggested. Two main reasons for this are discussed: (1) the types of representations and the relations between them differ across different domains, and (2) the instructional goals between domains and subsequent roles of the representations vary.
Growth and welfare in mixed health system financing with physician dual practice in a developing economy: a case of Indonesia
Authors: Alpaslan Barış , Lim King Yoong , Song Yan
Published: November 2020

Based on Indonesia’s hybrid BPJS Kesehatan health system, we analyze for welfare-optimal government financing strategy in an economy with a mixed health system using an endogenous growth framework with physician dual practice. We find the model solution to produce two vastly different regimes in terms of policy implications: a “high” public-sector congestion regime as in the benchmark case of Indonesia, and a “low” public-sector congestion, high capacity regime. In the former, welfare-optimal health financing strategy appears to be promoting private health service. In contrast, in the low-congestion, high capacity regime, a welfare-optimal strategy is to do the opposite of increasing government physician wage at the expense of private health subsidy. These results highlight the importance of developing a benchmarking system that measures the actual degree of congestion faced by the public health service in a developing economy, as it ultimately would influence the optimal health financing strategy to be pursued.
Model for estimating activity coefficients in binary and ternary ionic surfactant solutions
Authors: Calderón Silvia M. , Malila Jussi , Prisle Nønne L.
Published: November 2020

We introduce the CMC based Ionic Surfactant Activity model (CISA) to calculate activity coefficients in ternary aqueous solutions of an ionic surfactant and an inorganic salt. The surfactant can be either anionic or cationic and in the present development, the surfactant and inorganic salts share a common counterion. CISA incorporates micellization into the Pitzer–Debye–Hückel (PDH) framework for activities of mixed electrolyte solutions. To reduce computing requirements, a parametrization of the critical micelle concentration (CMC) is used to estimate the degree of micellization instead of explicit equilibrium calculations. For both binary and ternary systems, CISA only requires binary experimentally-based parameters to describe water–ion interactions and temperature–composition dependency of the CMC. The CISA model is intended in particular for atmospheric applications, where higher-order solution interaction parameters are typically not constrained by experiments and the description must be reliable across a wide range of compositions. We evaluate the model against experimental activity data for binary aqueous solutions of ionic surfactants sodium octanoate and sodium decanoate, as common components of atmospheric aerosols, and sodium dodecylsulfate, the most commonly used model compound for atmospheric surfactants. Capabilities of the CISA model to describe ternary systems are tested for the water–sodium decanoate–sodium chloride system, a common surrogate for marine background cloud condensation nuclei and to our knowledge the only atmospherically relevant system for which ternary activity data is available. For these systems, CISA is able to provide continuous predictions of activity coefficients both below and above CMC and in all cases gives an improved description of the water activity above the CMC, compared to the alternative model of Burchfield and Wolley [J. Phys. Chem., 88(10), 2149–2155 (1984)]. The water activity is a key parameter governing the formation and equilibrium growth of cloud droplets. The CISA model can be extended from the current form to include the effect of other inorganic salts with the existing database of binary PDH parameters and using appropriate mixing rules to account for ion specificity in the micellization process.
Probabilistic patterns of inundation and biogeomorphic changes due to sea-level rise along the northeastern U.S. Atlantic coast
Authors: Lentz Erika E. , Zeigler Sara L. , Thieler E. Robert , Plant Nathaniel G.
Published: November 2020

Context

Coastal landscapes evolve in response to sea-level rise (SLR) through a variety of geologic processes and ecological feedbacks. When the SLR rate surpasses the rate at which these processes build elevation and drive lateral migration, inundation is likely.

Objectives

To examine the role of land cover diversity and composition in landscape response to SLR across the northeastern United States.

Methods

Using an existing probabilistic framework, we quantify the probability of inundation, a measure of vulnerability, under different SLR scenarios on the coastal landscape. Resistant areas—wherein a dynamic response is anticipated—are defined as unlikely (p < 0.33) to inundate. Results are assessed regionally for different land cover types and at 26 sites representing varying levels of land cover diversity.

Results

Modeling results suggest that by the 2050s, 44% of low-lying, habitable land in the region is unlikely to inundate, further declining to 36% by the 2080s. In addition to a decrease in SLR resistance with time, these results show an increasing uncertainty that the coastal landscape will continue to evolve in response to SLR as it has in the past. We also find that resistance to SLR is correlated with land cover composition, wherein sites containing land cover types adaptable to SLR impacts show greater potential to undergo biogeomorphic state shifts rather than inundating with time.

Conclusions

Our findings support other studies that have highlighted the importance of ecological composition and diversity in stabilizing the physical landscape and suggest that flexible planning strategies, such as adaptive management, are particularly well suited for SLR preparation in diverse coastal settings.
Detecting emotions during a memory training assisted by a social robot for individuals with Mild Cognitive Impairment (MCI)
Authors: Palestra Giuseppe , Pino Olimpia
Published: November 2020

The attention towards robot-assisted therapies (RAT) had grown steadily in recent years particularly for patients with dementia. However, rehabilitation practice using humanoid robots for individuals with Mild Cognitive Impairment (MCI) is still a novel method for which the adherence mechanisms, indications and outcomes remain unclear. An effective computing represents a wide range of technological opportunities towards the employment of emotions to improve human-computer interaction. Therefore, the present study addresses the effectiveness of a system in automatically decode facial expression from video-recorded sessions of a robot-assisted memory training lasted two months involving twenty-one participants. We explored the robot’s potential to engage participants in the intervention and its effects on their emotional state. Our analysis revealed that the system is able to recognize facial expressions from robot-assisted group therapy sessions handling partially occluded faces. Results indicated reliable facial expressiveness recognition for the proposed software adding new evidence base to factors involved in Human-Robot Interaction (HRI). The use of a humanoid robot as a mediating tool appeared to promote the engagement of participants in the training program. Our findings showed positive emotional responses for females. Tasks affects differentially affective involvement. Further studies should investigate the training components and robot responsiveness.
Word-to-text integration in English as a second language reading comprehension
Authors: Mulder Evelien , van de Ven Marco , Segers Eliane , Krepel Alexander , de Bree Elise H. , de Jong Peter F. , Verhoeven Ludo
Published: November 2020

We assessed the relationship between word-to-text-integration (WTI) and reading comprehension in 7th grade students (n = 441) learning English as a second language (L2). The students performed a self-paced WTI reading task in Fall (T1) and Spring (T2), consisting of three text manipulation types (anaphora resolution, argument overlap, anomaly detection), divided in simple and complex passages. The passages contained proximate versus distant anaphora, explicit repetitions versus implicit inferences, and no anomalies versus anomalies. We first examined how WTI complexity was related to reading times on target, target plus one, and target plus two, controlling for word frequency, decoding fluency, gender, and age. Mixed-effects models showed shorter reading times on T2 than on T1 and for simple compared to complex passages, indicating improvement of L2 reading speed. Complexity affected WTI for our L2 learners, as was reflected by longer reading times on complex compared to simple argument overlap and anomaly detection passages. We then assessed whether reading comprehension could be predicted by WTI. Longer reading times on complex compared to simple argument overlap and anomaly detection passages predicted offline reading comprehension. These WTI-measures of complexity are thus indicators of WTI proficiency for novice L2 learners.
Forensic NMR metabolomics: one more arrow in the quiver
Authors: Locci Emanuela , Bazzano Giovanni , Chighine Alberto , Locco Francesco , Ferraro Ernesto , Demontis Roberto , d’Aloja Ernesto
Published: November 2020

Introduction

NMR metabolomics is increasingly used in forensics, due to the possibility of investigating both endogenous metabolic profiles and exogenous molecules that may help to describe metabolic patterns and their modifications associated to specific conditions of forensic interest.

Objectives

The aim of this work was to review the recent literature and depict the information provided by NMR metabolomics. Attention has been devoted to the identification of peculiar metabolic signatures and specific ante-mortem and post-mortem profiles or biomarkers related to different conditions of forensic concern, such as the identification of biological traces, the estimation of the time since death, and the exposure to drugs of abuse.

Results and Conclusion

The results of the described studies highlight how forensics can benefit from NMR metabolomics by gaining additional information that may help to shed light in several forensic issues that still deserve to be further elucidated.
Developing KASP Markers for Identification of Basmati Rice Varieties
Authors: Steele Katherine , Tulloch Mark Quinton , Burns Malcolm , Nader Werner
Published: November 2020

Authentication of Basmati rice has relied on microsatellite markers since 2004, but microsatellites cannot distinguish between all of the forty-one Basmati varieties approved in 2017. This study investigated whether single nucleotide polymorphisms (SNP) and insertion/deletion (InDel) variations developed into KASP™ (Kompetitive Allele Specific PCR; LGC Biosearch Technologies) could be used to distinguish between commercial Basmati varieties. Suitable loci were identified by comparing whole genome sequences of 120 diverse rice accessions. Sequences flanking these loci were standardized across a wide range of rice genomes to produce optimal KASP designs. We selected 364 KASP designs to use for genotyping; they were either near to informative microsatellite markers, within the Badh2 and Waxy genes, or distributed throughout the rice genome. Genotypes for 327 KASP were obtained with 255 loci revealing polymorphism in up to 41 samples of approved Basmati varieties and 20 non-Basmati varieties. The varieties genotyped had not been used in the KASP design process. KASP were able to distinguish between commercial Basmati varieties that could not be distinguished with currently available microsatellites. Thirty-seven Basmati varieties could be distinguished from all others with between 3 and 8 KASP markers out of a pool of 98 informative markers. A reduced set of 24 KASP markers could determine whether a sample belongs to one of eight family groups. All of the KASP markers used in this study can be purchased from LGC Biosearch Technologies. These markers have potential to be used by industry for routine testing and regulation.
The comparison of design airflow rates with dynamic and steady-state displacement models in varied dynamic conditions
Authors: Lastovets Natalia , Kosonen Risto , Jokisalo Juha
Published: November 2020

A temperature-based method is usually applied in displacement ventilation (DV) design when overheating is the primary indoor climate concern. Different steady-state models have been developed and implemented to calculate airflow rate in rooms with DV. However, in practical applications, the performance of DV depends on potentially dynamic parameters, such as strength, type and location of heat gains and changing heat gain schedule. In addition, thermal mass affects dynamically changing room air temperature. The selected steady-state and dynamic models were validated with the experimental results of a lecture room and an orchestra rehearsal room. Among the presented models, dynamic DV model demonstrated a capability to take into account the combination of dynamic parameters in typical applications of DV. The design airflow rate is calculated for the case studies of dynamic DV design in the modelled lecture room in both dynamic and steady-state conditions. In dynamic conditions of heavy construction in 2–4 hours occupancy periods, the actual airflow rate required could be 50% lower than the airflow rate calculated with the steady-state models. The difference between steady-state and dynamic multi-nodal model is most significant with heavyweight construction and short occupancy period (17%–28%). In cases with light construction, the dynamic DV model provides roughly the same airflow rates for four-hour occupancy period than the Mund’s model calculates. The dynamic model can significantly decrease the design airflow rate of DV, which can result in a reduction of investment costs and electrical consumption of fans.
Practical guidance for echocardiography for cancer therapeutics-related cardiac dysfunction
Authors:   Guideline Committee of the Japanese Society of Echocardiography, Onishi Tetsuari , Fukuda Yuko , Miyazaki Sakiko , Yamada Hirotsugu , Tanaka Hidekazu , Sakamoto Jiro , Daimon Masao , Izumi Chisato , Nonaka Akiko , Nakatani Satoshi , Akaishi Makoto
Published: November 2020

The prognosis of patients with cancer has improved due to an early diagnosis of cancer and advances in cancer treatment. There are emerging reports on cardiotoxicity in cancer treatment and on cardiovascular disease in cancer patients, from which cardiovascular disease has been recognized as a common cause of death among cancer survivors. This situation has led to the need for a medical system in which oncologists and cardiologists work together to treat patients. With the growing importance of onco-cardiology, the role of echocardiography in cancer care is rapidly expanding, but at present, the practice of echocardiography in clinical settings varies from institution to institution, and is empirical with no established systematic guidance. In view of these circumstances, we thought that brief guidance for clinical application was necessary and have therefore developed this guidance, although evidence in this field is still insufficient.
Fast MoS$$_2$$ thickness identification by transmission imaging
Authors: Neri Igor , López-Suárez Miquel , Caponi Silvia , Mattarelli Maurizio
Published: November 2020

Determining the thickness of a few-layer 2D material is a tough task that often involves complex and time consuming measurements. Here we discuss a rapid method for determining the number of layers of molybdenum disulfide, MoS$$_2$$

2

, flakes based on microscopic transmission imaging. By analyzing the contrast of the red, blue and green channels of the flake image against the background, we show that it is possible to unequivocally determine the number of layers. The presented method is based on the light absorption properties of MoS$$_2$$

2

and its validity is confirmed by micro-Raman measurements. The main advantage of this method against traditional methods is to quickly determine the thickness of the material in the early stages of the experimental process with low cost apparatus.
Seasonal and spatial shifts in feral cat predation on native seabirds vs. non-native rats on Mikura Island, Japan
Authors: Azumi Sarara , Watari Yuya , Oka Nariko , Miyashita Tadashi
Published: November 2020

Understanding how invasive predators impact native species is essential for the development of effective control strategies, especially in insular environments where alternative non-native prey species exist. We examined seasonal and spatial shifts in diet of feral cat Felis silvestris catus focusing on the predation on native streaked shearwaters, Calonectris leucomelas, and introduced rats, Rattus rattus and R. norvegicus, which are alternative prey to shearwaters, on Mikura Island, Japan. Streaked shearwaters breed at low elevations on the island from spring to autumn, whereas rats inhabit the island throughout the year, which makes them an alternative prey when native shearwaters are absent. Fecal analysis revealed that feral cats dramatically shifted their diets from introduced rats in winter to streaked shearwaters in seabird-season in low elevation areas of the island, while cats preyed on rats throughout the year at high altitudes on the island. This finding suggests that feral cats selectively prey on shearwaters. This is probably because of their large body size and less cautious behavior, and because introduced rats sustain the cat population when shearwaters are absent. The number of streaked shearwaters killed was estimated to be 313 individuals per cat per year, which represents an indication of top-down effects of feral cats on streaked shearwaters. Further studies on the demographic parameters and interspecific interactions of the three species are required to enable effective cat management for the conservation of streaked shearwaters on this island.
Genetics of Amyotrophic Lateral Sclerosis
Authors: Gregory Jenna M. , Fagegaltier Delphine , Phatnani Hemali , Harms Matthew B.
Published: November 2020

Purpose of Review

Amyotrophic lateral sclerosis and frontotemporal dementia (ALS-FTD) spectrum disorder is a rare fatal disease with strong genetic influences. The implementation of short-read sequencing methodologies in increasingly large patient cohorts has rapidly expanded our knowledge of the complex genetic architecture of the disease. We aim to convey the broad history of ALS gene discovery as context for a focused review of 11 ALS gene associations reported over the last 5 years. We also summarize the current level of genetic evidence for all previously reported genes.

Recent Findings

The history of ALS gene discovery has occurred in at least four identifiable phases, each powered by different technologies and scale of investigation. The most recent epoch, benefitting from population-scale genome data, large international consortia, and low-cost sequencing, has yielded 11 new gene associations. We summarize the current level of genetic evidence supporting these ALS genes, highlighting any genotype-phenotype or genotype-pathology correlations, and discussing preliminary understanding of molecular pathogenesis. This era has also raised uncertainty around prior ALS-associated genes and clarified the role of others.

Our understanding of the genetic underpinning of ALS has expanded rapidly over the last 25 years and has led directly to the clinical application of molecularly driven therapies. Ongoing sequencing efforts in ALS will identify new causative and risk factor genes while clarifying the status of genes reported in prior eras of research.
Effect of drought length on the performance of cabbage (Brassica oleracea var capitata) in the forest-savannah transition zone, Ghana
Authors: Ackah E. , Kotei R.
Published: November 2020

The drought tolerance of Cabbage, Brassica oleracea var capitata (Oxylus) was investigated by subjecting the initial, development and mid growth stages to varying drought lengths of 5, 7, 9, 11, 13 and 15 days in a 7 × 3 factorial experiment to determine the effect of each drought period at each growth stage on growth and yield. Data on number of opened leaves/plant, leaf area index (LAI), leaf chlorophyll content, head size and yield/ha were recorded due to drought effect at each growth stage. Analysis of variance at 5% probability level indicated that drought periods of 5–15 days at the initial stage significantly reduced number of leaves, LAI and head size. Significant reduction in chlorophyll content and yield were due to drought beyond 11 and 5 days respectively at the initial stage. Number of opened leaves increased significantly with increasing drought length at the development stage; critical at 11 days drought. Significant reductions in LAI at the development and mid stages were critical at 11 and 9 days respectively whilst chlorophyll content was significantly reduced at both the development and mid stages by all drought periods. Significant reduction in head size at the development and mid stages was critical at 7 days. Generally, the drought tolerance of cabbage increased from the initial to the mid-stage. To maintain economic yields of cabbage in the forest-savannah transition zone, the initial and development stages could tolerate drought stress up to 5 days whilst the mid stage could tolerate up to 7 days.
Infection control measures in times of antimicrobial resistance: a matter of solidarity
Authors: Rump Babette , Timen Aura , Hulscher Marlies , Verweij Marcel
Published: November 2020

Control measures directed at carriers of multidrug-resistant organisms are traditionally approached as a trade-off between public interests on the one hand and individual autonomy on the other. We propose to reframe the ethical issue and consider control measures directed at carriers an issue of solidarity. Rather than asking “whether it is justified to impose strict measures”, we propose asking “how to best care for a person’s carriership and well-being in ways that do not imply an unacceptable risk for others?”. A solidarity approach could include elevating baseline levels of precaution measures and accepting certain risks in cases where there is exceptionally much at stake. A generous national compensation policy that also covers for costs related to dedicated care is essential in a solidarity approach. An additional benefit of reframing the questions is that it helps to better acknowledge that being subjected to control measures is a highly personal matter.