bluuebunny/crossref_metadata_2025 huggingface dataset:
The promise of pulsed field ablation and the challenges ahead
Authors: Iyengar Shruti Krishna , Iyengar Sumedh , Srivathsan Komandoor
Published: October 2023

For many years, guidelines have suggested thermal ablation for the treatment of atrial fibrillation. Thermal ablation involves the destruction of tissue, leading to multiple complications. This ablation technique has been tried and tested, however, newer techniques are being investigated in order to avoid these complications. Pulsed field ablation, a nonthermal method of tissue ablation, is being explored as a more safe and efficient way to treat atrial fibrillation. This mini review aims to highlight the mechanisms of pulsed field ablation, its history and evolution, previous studies showing its efficacy, its major challenges and pitfalls, and future advancements to overcome these challenges. This method of ablation could potentially revolutionize the treatment of atrial fibrillation and prevent recurrences, thereby making it easier for the physicians and patients involved.
Developmental, cytogenetic and epigenetic consequences of removing complex proteins and adding melatonin during in vitro maturation of bovine oocytes
Authors: Tutt Desmond A. R. , Guven-Ates Gizem , Kwong Wing Yee , Simmons Rob , Sang Fei , Silvestri Giuseppe , Canedo-Ribeiro Carla , Handyside Alan H. , Labrecque Remi , Sirard Marc-André , Emes Richard D. , Griffin Darren K. , Sinclair Kevin D.
Published: October 2023

Background

In vitro maturation (IVM) of germinal vesicle intact oocytes prior to in vitro fertilization (IVF) is practiced widely in animals. In human assisted reproduction it is generally reserved for fertility preservation or where ovarian stimulation is contraindicated. Standard practice incorporates complex proteins (CP), in the form of serum and/or albumin, into IVM media to mimic the ovarian follicle environment. However, the undefined nature of CP, together with batch variation and ethical concerns regarding their origin, necessitate the development of more defined formulations. A known component of follicular fluid, melatonin, has multifaceted roles including that of a metabolic regulator and antioxidant. In certain circumstances it can enhance oocyte maturation. At this stage in development, the germinal-vesicle intact oocyte is prone to aneuploidy and epigenetic dysregulation.

Objectives

To determine the developmental, cytogenetic and epigenetic consequences of removing CP and including melatonin during bovine IVM.

Materials and methods

The study comprised a 2 x 2 factorial arrangement comparing (i) the inclusion or exclusion of CP, and (ii) the addition (100 nM) or omission of melatonin, during IVM. Cumulus-oocyte complexes (COCs) were retrieved from stimulated cycles. Following IVM and IVF, putative zygotes were cultured to Day 8 in standard media. RNAseq was performed on isolated cumulus cells, cytogenetic analyses (SNP-based algorithms) on isolated trophectoderm cells, and DNA methylation analysis (reduced representation bisulfite sequencing) on isolated cells of the inner-cell mass.

Results

Removal of CP during IVM led to modest reductions in blastocyst development, whilst added melatonin was beneficial in the presence but detrimental in the absence of CP. The composition of IVM media did not affect the nature or incidence of chromosomal abnormalities but cumulus-cell transcript expression indicated altered metabolism (primarily lipid) in COCs. These effects preceded the establishment of distinct metabolic and epigenetic signatures several days later in expanded and hatching blastocysts.

Conclusions

These findings highlight the importance of lipid, particularly sterol, metabolism by the COC during IVM. They lay the foundation for future studies that seek to develop chemically defined systems of IVM for the generation of transferrable embryos that are both cytogenetically and epigenetically normal.
Protection from infection and reinfection due to the Omicron BA.1 variant in care homes
Authors: Choudhry Saher , Rowland Thomas A. J. , McClelland Kamil , Renz Erik , Iyanger Nalini , Chow J Yimmy , Aiano Felicity , Ladhani Shamez N. , Jeffery-Smith Anna , Andrews Nick J. , Zambon Maria
Published: October 2023

Introduction

Following the emergence of SARS-CoV-2 in 2020, care homes were disproportionately impacted by high mortality and morbidity of vulnerable elderly residents. Non-pharmaceutical interventions (NPIs) and improved infection control measures together with vaccination campaigns have since improved outcomes of infection. We studied the utility of past infection status, recent vaccination and anti-S antibody titres as possible correlates of protection against a newly emergent Omicron variant infection.

Methods

Prospective longitudinal surveillance of nine sentinel London care homes from April 2020 onwards found that all experienced COVID-19 outbreaks due to Omicron (BA.1) during December 2021 and January 2022, despite extensive prior SARS-CoV-2 exposure and high COVID-19 vaccination rates, including booster vaccines (>70% residents, >40% staff).

Results

Detailed investigation showed that 46% (133/288) of Omicron BA.1 infections were SARS-CoV-2 reinfections. Two and three COVID-19 vaccine doses were protective against Omicron infection within 2-9 weeks of vaccination, though protection waned from 10 weeks post-vaccination. Prior infection provided additional protection in vaccinated individuals, approximately halving the risk of SARS-CoV-2 infection.

Discussion

Anti-S antibody titre showed a dose-dependent protective effect but did not fully account for the protection provided by vaccination or past infection, indicating that other mechanisms of protection are also involved.
The effects of Phycocyanobilin on experimental arthritis involve the reduction in nociception and synovial neutrophil infiltration, inhibition of cytokine production, and modulation of the neuronal proteome
Authors: Marín-Prida Javier , Rodríguez-Ulloa Arielis , Besada Vladimir , Llopiz-Arzuaga Alexey , Batista Nathália Vieira , Hernández-González Ignacio , Pavón-Fuentes Nancy , Marciano Vieira Érica Leandro , Falcón-Cama Viviana , Acosta Emilio F. , Martínez-Donato Gillian , Cervantes-Llanos Majel , Lingfeng Dai , González Luis J. , Fernández-Massó Julio Raúl , Guillén-Nieto Gerardo , Pentón-Arias Eduardo , Amaral Flávio Almeida , Teixeira Mauro Martins , Pentón-Rol Giselle
Published: October 2023

Introduction

The antinociceptive and pharmacological activities of C-Phycocyanin (C-PC) and Phycocyanobilin (PCB) in the context of inflammatory arthritis remain unexplored so far. In the present study, we aimed to assess the protective actions of these compounds in an experimental mice model that replicates key aspects of human rheumatoid arthritis.

Methods

Antigen-induced arthritis (AIA) was established by intradermal injection of methylated bovine serum albumin in C57BL/6 mice, and one hour before the antigen challenge, either C-PC (2, 4, or 8 mg/kg) or PCB (0.1 or 1 mg/kg) were administered intraperitoneally. Proteome profiling was also conducted on glutamate-exposed SH-SY5Y neuronal cells to evaluate the PCB impact on this key signaling pathway associated with nociceptive neuronal sensitization.

Results and discussion

C-PC and PCB notably ameliorated hypernociception, synovial neutrophil infiltration, myeloperoxidase activity, and the periarticular cytokine concentration of IFN-γ, TNF-α, IL-17A, and IL-4 dose-dependently in AIA mice. In addition, 1 mg/kg PCB downregulated the gene expression for T-bet, RORγ, and IFN-γ in the popliteal lymph nodes, accompanied by a significant reduction in the pathological arthritic index of AIA mice. Noteworthy, neuronal proteome analysis revealed that PCB modulated biological processes such as pain, inflammation, and glutamatergic transmission, all of which are involved in arthritic pathology.

Conclusions

These findings demonstrate the remarkable efficacy of PCB in alleviating the nociception and inflammation in the AIA mice model and shed new light on mechanisms underlying the PCB modulation of the neuronal proteome. This research work opens a new avenue to explore the translational potential of PCB in developing a therapeutic strategy for inflammation and pain in rheumatoid arthritis.
A systematic review and meta-analysis of the kynurenine pathway of tryptophan metabolism in rheumatic diseases
Authors: Mangoni Arduino A. , Zinellu Angelo
Published: October 2023

There is an increasing interest in the pathophysiological role of the kynurenine pathway of tryptophan metabolism in the regulation of immune function and inflammation. We sought to address the link between this pathway and the presence rheumatic diseases (RD) by conducting a systematic review and meta-analysis of studies reporting the plasma or serum concentrations of tryptophan, kynurenine, and other relevant metabolites in RD patients and healthy controls. We searched electronic databases for relevant articles published between inception and the 30th of June 2023. Risk of bias and certainty of evidence were assessed using the Joanna Briggs Institute Critical Appraisal Checklist and the Grades of Recommendation, Assessment, Development and Evaluation Working Group system. In 24 studies selected for analysis, compared to controls, RD patients had significantly lower tryptophan (standard mean difference, SMD= -0.71, 95% CI -1.03 to -0.39, p<0.001; I2 = 93.6%, p<0.001; low certainty of evidence), and higher kynurenine (SMD=0.69, 95% CI 0.35 to 1.02, p<0.001; I2 = 93.2%, p<0.001; low certainty), kynurenine to tryptophan ratios (SMD=0.88, 95% CI 0.55 to 1.21, p<0.001; I2 = 92.9%, p<0.001; moderate certainty), 3-hydroxykynurenine (SMD=0.74, 95% CI 0.30 to 1.18, p=0.001; I2 = 87.7%, p<0.001; extremely low certainty), and quinolinic acid concentrations (SMD=0.71, 95% CI 0.31 to 1.11, p<0.001; I2 = 88.1%, p<0.001; extremely low certainty). By contrast, there were non-significant between-group differences in kynurenic acid, 3-hydroxyanthranilic acid, kynurenic acid to kynurenine ratio, or quinolinic acid to kynurenine acid ratio. In meta-regression, the SMD of tryptophan, kynurenine, and kynurenine to tryptophan ratio were not associated with age, publication year, sample size, RD duration, C-reactive protein, or use of anti-rheumatic drugs and corticosteroids. In subgroup analysis, the SMD of tryptophan, kynurenine, and kynurenine to tryptophan ratio was significant across different types of RD, barring rheumatoid arthritis. Therefore, we have observed significant alterations in tryptophan, kynurenine, 3-hydroxykynurenine, and quinolinic acid concentrations in RD patients. Further research is warranted to determine whether these biomarkers can be useful for diagnosis and management in this patient group. (PROSPERO registration number: CRD CRD42023443718).

Systematic review registration

https://www.crd.york.ac.uk/prospero, identifier CRD CRD42023443718.
Vascular normalization: reshaping the tumor microenvironment and augmenting antitumor immunity for ovarian cancer
Authors: Yu Ping , Wang Yaru , Yuan Dahai , Sun Yunqin , Qin Shuang , Li Tianye
Published: October 2023

Ovarian cancer remains a challenging disease with limited treatment options and poor prognosis. The tumor microenvironment (TME) plays a crucial role in tumor growth, progression, and therapy response. One characteristic feature of the TME is the abnormal tumor vasculature, which is associated with inadequate blood perfusion, hypoxia, and immune evasion. Vascular normalization, a therapeutic strategy aiming to rectify the abnormal tumor vasculature, has emerged as a promising approach to reshape the TME, enhance antitumor immunity, and synergize with immunotherapy in ovarian cancer. This review paper provides a comprehensive overview of vascular normalization and its potential implications in ovarian cancer. In this review, we summarize the intricate interplay between anti-angiogenesis and immune modulation, as well as ICI combined with anti-angiogenesis therapy in ovarian cancer. The compelling evidence discussed in this review contributes to the growing body of knowledge supporting the utilization of combination therapy as a promising treatment paradigm for ovarian cancer, paving the way for further clinical development and optimization of this therapeutic approach.
Targeting post-translational modifications of Foxp3: a new paradigm for regulatory T cell-specific therapy
Authors: Riaz Farooq , Huang Zhihui , Pan Fan
Published: October 2023

A healthy immune system is pivotal for the hosts to resist external pathogens and maintain homeostasis; however, the immunosuppressive tumor microenvironment (TME) damages the anti-tumor immunity and promotes tumor progression, invasion, and metastasis. Recently, many studies have found that Foxp3+ regulatory T (Treg) cells are the major immunosuppressive cells that facilitate the formation of TME by promoting the development of various tumor-associated cells and suppressing the activity of effector immune cells. Considering the role of Tregs in tumor progression, it is pivotal to identify new therapeutic drugs to target and deplete Tregs in tumors. Although several studies have developed strategies for targeted deletion of Treg to reduce the TME and support the accumulation of effector T cells in tumors, Treg-targeted therapy systematically affects the Treg population and may lead to the progression of autoimmune diseases. It has been understood that, nevertheless, in disease conditions, Foxp3 undergoes several definite post-translational modifications (PTMs), including acetylation, glycosylation, phosphorylation, ubiquitylation, and methylation. These PTMs not only elevate or mitigate the transcriptional activity of Foxp3 but also affect the stability and immunosuppressive function of Tregs. Various studies have shown that pharmacological targeting of enzymes involved in PTMs can significantly influence the PTMs of Foxp3; thus, it may influence the progression of cancers and/or autoimmune diseases. Overall, this review will help researchers to understand the advances in the immune-suppressive mechanisms of Tregs, the post-translational regulations of Foxp3, and the potential therapeutic targets and strategies to target the Tregs in TME to improve anti-tumor immunity.
Distribution and structure of deep-sea demersal fish assemblages across the central and western Pacific Ocean using data from undersea imagery
Authors: Selig Gina M. , Drazen Jeffrey C. , Auster Peter J. , Mundy Bruce C. , Kelley Christopher D.
Published: October 2023

Demersal deep-sea fish assemblages from islands and seamounts are poorly described, even in the Hawaiian archipelago. Knowledge across all depths, in similar settings, is even sparser for other archipelagos in the central and western Pacific. However, recent remotely operated vehicle (ROV) explorations and archived video from human-occupied submersible dives conducted by the Hawai`i Undersea Research Laboratory (HURL) provide an opportunity to explore the structure of these assemblages. Here we describe demersal fish assemblages across the central and western Pacific, including in four Marine National Monuments, and examine the relationship of the assemblages to depth and environmental conditions. We used data collected from 227 underwater vehicle dives resulting in the identification of 24,837 individuals belonging to 89 families and 175 genera. The most frequently occurring genera at depths of 250-500 m were Epigonus, Setarches, Polymixia, and Antigonia, between 500-1000 m were Chlorophthalmus, Aldrovandia, and Neocyttus, and between 1000-3000 m were Synaphobranchus, Kumba, Halosaurus, Ilyophis, and Ipnops. There are strong changes in the fish assemblages with depth and region, and assemblages become more similar between regions with greater depth. Depth and region explained the most variance in assemblage structure followed by seafloor particulate organic carbon flux (a food supply proxy), concentrations of dissolved oxygen, and salinity. The Line Islands and Tokelau Ridge had the highest values of seafloor particulate organic carbon flux for all depth zones investigated (250-3000 m) and the highest abundance of fishes at 250-500 m and 500-1000 m, respectively. Taxon accumulation curves indicated that diversity at the genus level within all regions and depth bins (except 1000-2000 m and 2000-3000 m) had not been reached with the existing sampling effort. However, when combining samples from all regions, diversity generally appeared to decrease with depth. Overall, this study demonstrates that there are significant regional differences in the composition of the deep-sea fish fauna as well as differences across depth. Such distribution patterns suggest that the four Marine National Monuments (Papahānaumokuākea, Marianas Trench, Pacific Remote Islands, and Rose Atoll Marine National Monuments, encompassing an area of 3,063,223 km2) are not replicates of diversity, but complementary components of the regional fauna.
Optimization of the k-nearest-neighbors model for summer Arctic Sea ice prediction
Authors: Lin Yongcheng , Yang Qinghua , Li Xuewei , Yang Chao-Yuan , Wang Yiguo , Wang Jiuke , Liu Jingwen , Chen Sizhe , Liu Jiping
Published: October 2023

The Arctic sea ice plays a significant role in climate-related processes and has a considerable effect on humans, however accurately predicting the Arctic sea ice concentration is still challenging. Recently, with the rise and development of artificial intelligence, big data technology, machine learning has been widely used in the field of sea ice prediction. In this study, we utilized a sea ice concentration dataset obtained from satellite remote sensing and applied the k-nearest-neighbors (Ice-kNN) machine learning model to forecast the summer Arctic sea ice concentration and extent on 122 days prediction. Based on the physical characteristics of summer sea ice, different algorithms are employed to optimize the prediction model. A drift-ice correction algorithm is designed to address the unrealistic drift ice around the sea ice edge, and a distance function combined with the spatial pattern is proposed to enhance similarity detection. Deseasonalized and detrended sea ice datasets and an expanded training library are also utilized to improve model performance. Furthermore, sensitivity analysis reveals a positive impact of net surface heat flux on sea ice prediction. The modified Ice-kNN model outperforms climatological and anomaly persistence predictions, demonstrating its applicability to predicting summer Arctic sea ice. The September sea ice extent hindcasts of the modified Ice-kNN model are compared to a variety of models submitted to the Sea Ice Prediction Network, underscoring its potential to improve predictive skill for Arctic sea ice.
A Wasserstein generative adversarial network with gradient penalty for active sonar signal reverberation suppression
Authors: Wang Zhen , Zhang Hao , Huang Wei , Chen Xiao , Tang Ning , An Yuan
Published: October 2023

Reverberation is the primary background interference of active sonar systems in shallow water environments, affecting target position detection accuracy. Reverberation suppression is a signal processing technique used to improve the clarity and accuracy of received signals by eliminating the echoes, reverberations, and noise that occur during underwater propagation. Existing reverberation suppression methods include algorithms based on Time-Frequency domain processing, noise reduction, adaptive filtering, and spectral subtraction, but their performance in high-reverberation environments (echo of small targets) still does not meet the requirements of target detection. To address the impact of high reverberation environments, we propose a structural suppression method based on the Wasserstein gradient penalty generative adversarial network (RSWGAN-GP). The reverberation suppression generation network uses a one-dimensional convolutional network structure to process normalized time-domain signals and achieves the reconstruction of the reverberation signal through Encoder-Decoder. The proposed method is verified through accurate and effective data collection during sea trials. Comparative results show that RSWGAN-GP effectively suppresses reverberation in observation signals with multiple bright spots, improving the signal-to-reverberation ratio by approximately 10 dB compared to other excellent algorithms and enhancing the information analysis and feature extraction capabilities of active sonar signals.
Risk zonation of submarine geological hazards in the Chengdao area of the Yellow River subaqueous delta
Authors: Wang Zhenhao , Du Xing , Sun Yongfu , Song Yupeng , Dong Lifeng , Zhou Qikun , Jiang Weida
Published: October 2023

Submarine geological disasters occur frequently in the Chengdao area of the Yellow River subaqueous delta, which seriously threaten the safe operation of marine engineering in the region. Therefore, it is of great significance to carry out risk zonation for this region. The current study presents a detailed assessment of risk zones related to submarine geological hazards in this region based on analytical hierarchy process (AHP). The system of assessment index for risk zonation was established with four aspects of hydrodynamic condition, engineering geological environment, disaster geological conditions and human engineering activities. Eight geological hazard evaluation factors were selected and the distribution characteristics of each evaluation factor were discussed in detail by combining qualitative analysis and quantitative calculation. The risk level of submarine geological hazards in the Chengdao area are divided into four types: low risk, relatively low risk, relatively high risk, and high risk. The results show that the areas with a high geological hazard risk in this region are mainly distributed in the areas with water depths of 9–12 m, where the hydrodynamic effect is strong, with many human engineering activities, and seriously suffered from geologic hazards. The study results can provide scientific basis for engineering construction and hazard prevention in the Chengdao area.
Accessible art in healthcare facilities: exploring perspectives of healthcare art for visually impaired people
Authors: Palityka Daryia , Chrysikou Evangelia , Murtagh Niamh
Published: October 2023

Introduction

Art in healthcare facilities shows promising results in improving patients' health and well-being and, as such, meets the WHO's definition of health technology. Yet, it remains unclear if healthcare art equally benefits all users. Given the growing number of visually impaired people (VIP), it is valuable to determine whether healthcare art is accessible to VIP and to explore strategies for improving it.

Methods

This study employed a mixed methodology, which included (1) secondary research of 25 cases of healthcare art programmes to identify the presence of accessible art in healthcare facilities and the practices that influence it; (2) review of thirty-one Health Building Notes and four supplementary British guidelines on healthcare art to discover if the accessibility of art is required and identify which recommendations influence it; and (3) interview surveys of healthcare art practitioners from three London NHS Trusts to identify opportunities to increase arts accessibility.

Results and discussion

The evidence showed that healthcare art programmes were mostly inaccessible to VIP. Most healthcare art programmes did not involve VIP in the commissioning process and, thus, lacked procedures that could facilitate accessibility. There were not enough recommendations in the healthcare facility guidelines to support the accessibility of arts for VIP. The recommendations on artwork in healthcare facility guidelines could increase accessibility if particular conditions were met. Interviews with NHS trusts in London revealed numerous opportunities to improve arts accessibility for healthcare art programmes.
Aromatic compounds depurative and plant growth promotion rhizobacteria abilities of Allenrolfea vaginata (Amaranthaceae) rhizosphere microbial communities from a solar saltern hypersaline soil
Authors: Rodríguez-Valdecantos Gustavo , Torres-Rojas Felipe , Muñoz-Echeverría Sofía , del Rocío Mora-Ruiz Merit , Rosselló-Móra Ramon , Cid-Cid Luis , Ledger Thomas , González Bernardo
Published: October 2023

Introduction

This work investigates whether rhizosphere microorganisms that colonize halophyte plants thriving in saline habitats can tolerate salinity and provide beneficial effects to their hosts, protecting them from environmental stresses, such as aromatic compound (AC) pollution.

Methods

To address this question, we conducted a series of experiments. First, we evaluated the effects of phenol, tyrosine, 4-hydroxybenzoic acid, and 2,4-dichlorophenoxyacetic (2,4-D) acids on the soil rhizosphere microbial community associated with the halophyteAllenrolfea vaginata. We then determined the ability of bacterial isolates from these microbial communities to utilize these ACs as carbon sources. Finally, we assessed their ability to promote plant growth under saline conditions.

Results

Our study revealed that each AC had a different impact on the structure and alpha and beta diversity of the halophyte bacterial (but not archaeal) communities. Notably, 2,4-D and phenol, to a lesser degree, had the most substantial decreasing effects. The removal of ACs by the rhizosphere community varied from 15% (2,4-D) to 100% (the other three ACs), depending on the concentration.Halomonasisolates were the most abundant and diverse strains capable of degrading the ACs, with strains ofMarinobacter,Alkalihalobacillus,Thalassobacillus,Oceanobacillus, and the archaeaHaladaptatusalso exhibiting catabolic properties. Moreover, our study found that halophile strains Halomonas sp. LV-8T andMarinobactersp. LV-48T enhanced the growth and protection ofArabidopsis thalianaplants by 30% to 55% under salt-stress conditions.

Discussion

These results suggest that moderate halophile microbial communities may protect halophytes from salinity and potential adverse effects of aromatic compounds through depurative processes.
m5U-GEPred: prediction of RNA 5-methyluridine sites based on sequence-derived and graph embedding features
Authors: Xu Zhongxing , Wang Xuan , Meng Jia , Zhang Lin , Song Bowen
Published: October 2023

5-Methyluridine (m5U) is one of the most common post-transcriptional RNA modifications, which is involved in a variety of important biological processes and disease development. The precise identification of the m5U sites allows for a better understanding of the biological processes of RNA and contributes to the discovery of new RNA functional and therapeutic targets. Here, we present m5U-GEPred, a prediction framework, to combine sequence characteristics and graph embedding-based information for m5U identification. The graph embedding approach was introduced to extract the global information of training data that complemented the local information represented by conventional sequence features, thereby enhancing the prediction performance of m5U identification. m5U-GEPred outperformed the state-of-the-art m5U predictors built on two independent species, with an average AUROC of 0.984 and 0.985 tested on human and yeast transcriptomes, respectively. To further validate the performance of our newly proposed framework, the experimentally validated m5U sites identified from Oxford Nanopore Technology (ONT) were collected as independent testing data, and in this project, m5U-GEPred achieved reasonable prediction performance with ACC of 91.84%. We hope that m5U-GEPred should make a useful computational alternative for m5U identification.
Role and interaction of bacterial sphingolipids in human health
Authors: Bai Xiaoye , Ya Ru , Tang Xiaoyu , Cai Mingwei
Published: October 2023

Sphingolipids, present in both higher animals and prokaryotes, involving in cell differentiation, pathogenesis and apoptosis in human physiological health. With increasing attention on the gut microbiome and its impact on wellbeing, there is a renewed focus on exploring bacterial sphingolipids. This review aims to consolidate the current understanding of bacterial sphingolipids and their impact on host health. Compared to mammalian sphingolipids, bacterial sphingolipids are characterized by odd chain lengths due to the presence of branched alkyl chains. Additionally, intestinal microbial sphingolipids can migrate from the gut to various host organs, affecting the immune system and metabolism. Furthermore, the intricate interplay between dietary sphingolipids and the gut microbiota is explored, shedding light on their complex relationship. Despite limited knowledge in this area, this review aims to raise awareness about the importance of bacterial sphingolipids and further our understanding of more uncharacterized bacterial sphingolipids and their significant role in maintaining host health.
Endovascular treatment for young patients with acute large vessel occlusion stroke in China: analysis of the ANGEL-ACT registry
Authors: Han Bin , Raynald  , Sun Dapeng , Tong Xu , Jia Baixue , Wang Anxin , Mo Dapeng , Gao Feng , Ma Ning , Nguyen Thanh N. , Miao Zhongrong
Published: October 2023

Background

The incidence of acute ischemic stroke caused by large vessel occlusion is relatively infrequent in the young adult population. We sought to evaluate their clinical outcomes after endovascular treatment (EVT) and stroke etiology compared with older patients.

Methods

We examined data from the ANGEL-ACT registry, a nationwide study in China focusing on EVT for acute ischemic stroke. We compared two age groups: <50 years old and ≥50 years old. Our analysis focused on outcome measures such as the 90-day modified Rankin Scale (mRS) score, mortality, and symptomatic intracranial hemorrhage (sICH). We adjusted for confounding variables.

Results

We included 1,691 patients, and 216 patients (13%) were <50 years old. Young patients had lower median National Institutes of Health Stroke Scale (NIHSS) scores (14 vs. 17, P < 0.001) and fewer cardiovascular comorbidities than older patients. Underlying intracranial atherosclerosis disease (ICAD) was higher in young patients (39.4 vs. 28.7%, P = 0.001). Clinical outcome was less favorable in older compared to younger patients (mRS shift: 0.76 [95% confidence interval (CI), 0.58–0.99]); functional independence [mRS score 0–2] 61% vs. 39% (adjusted odds ratio (OR), 0.7 [95% CI, 0.51–0.97]). Mortality and sICH did not differ between groups. Onset to puncture time (OTP) was longer in young patients (357 min vs. 294 min, P = 0.001).

Conclusion

An estimated 13% of patients who underwent endovascular thrombectomy for acute ischemic stroke were <50 years old. Symptomatic underlying ICAD was more prevalent in the younger patient population. Despite a prehospital delay, younger patients exhibited more favorable outcomes than their older counterparts.
Biopsy-proven acute tubulointerstitial nephritis in patients treated with immune checkpoint inhibitors: a pooled analysis of case reports
Authors: Esposito Pasquale , Bottini Annarita , Lecini Elvina , Cappadona Francesca , Piaggio Michela , Macciò Lucia , Genova Carlo , Viazzi Francesca
Published: October 2023

Introduction

Acute kidney injury (AKI) in cancer patients receiving immune checkpoint inhibitors (ICIs) may recognize multiple causes. Here, we reviewed cases of biopsy-proven acute tubulointerstitial nephritis (ATIN) to describe the clinical characteristics and outcomes of this condition.

Method

We conducted a pooled analysis of clinical cases of ICI-related biopsy-proven ATIN up to 1 May 2022. We collected data on clinical characteristics, AKI, biopsy findings, laboratory examinations, and renal outcomes.

Results

Eighty-five patients (61.4 ± 19 years, 56 male) were evaluated. Melanoma was the most prevalent diagnosis (51%), followed by non-small cell lung cancer (30%). ICI treatment consisted of PD-1, PDL-1 (nivolumab, pembrolizumab, atezolizumab), and CTLA-4 inhibitors (i) (ipilimumab) or combination PD-1i+CTLA4i. Renal toxicity developed after a median of four cycles of therapy. Fifty-one patients (65.5%) developed the most severe form of AKI- stage 3, including five patients requiring dialysis. All the 19 patients treated with dual ICI blockade developed AKI-stage 3, compared with 29 patients out of the 60 receiving a single agent (p<0.001). Most events were managed with corticosteroids associated with ICI withdrawal. In 15 patients ICI was restarted, but in six (40%) AKI recurred. Overall, 32 patients (40%) presented a complete renal recovery, which chance was inversely associated with dual ICI blockade (OR 0.15, 95CI 0.03-0.7, p=0.01).

Conclusion

ICI-related ATIN may develop late after the therapy initiation, presenting as severe AKI, particularly in patients with dual ICI blockade. Although this complication may be partially reversible, concerns remain about the renal function sequelae and the possibility of restarting ICI treatment.
IGF2BP2 promotes head and neck squamous carcinoma cell proliferation and growth via the miR-98-5p/PI3K/Akt signaling pathway
Authors: Yu Dan , Xiao Zhenlong , Zou Zhefei , Lin Ling , Li Jing , Tan Jian , Chen Wei
Published: October 2023

Introduction

As a N6-methyladenosine reader protein, Insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2) is a critical player in tumor progression and metastasis. However, its specific function in head and neck squamous carcinoma (HNSCC) has yet to be determined. The present study aimed to determine the role of IGF2BP2 in HNSCC.

Methods

The expression of IGF2BP2 in HNSCC was analyzed using The Cancer Genome Atlas (TCGA) dataset and detected in HNSCC tissues and cells, respectively. Gain- and loss- of function methods were employed to study the effects of IGF2BP2 on HNSCC cell proliferation and tumorigenesis in vitro and in vivo. MicroRNAs (miRNAs) regulating IGF2BP2 were predicted using online tools and confirmed experimentally.

Results

We showed augmented IGF2BP2 expression in HNSCC, which correlated with poor clinical outcomes. Functional studies showed that IGF2BP2 promoted HNSCC cell proliferation by facilitating cell cycle progression while inhibiting apoptosis. We further demonstrated that IGF2BP2 could enhance HNSCC cell tumorigenesis in vivo. Mechanistically, our data revealed that miR-98-5p could directly target IGF2BP2. The interplay between IGF2BP2 and miR-98-5p is essential to drive the progression of HNSCC via the phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K)-protein kinase B (Akt) pathway signaling pathway.

Discussion

The current study revealed the oncogenic role of IGF2BP2 and provided insights into its potential mechanism in HNSCC tumorigenesis. Additionally, IGF2BP2 might represent a promising therapeutic target and serve as prognostic biomarker in patients with HNSCC.
A comparative study on the features of breast sclerosing adenosis and invasive ductal carcinoma via ultrasound and establishment of a predictive nomogram
Authors: Li Yuan , Wei Xiu-liang , Pang Kun-kun , Ni Ping-juan , Wu Mei , Xiao Juan , Zhang Lu-lu , Zhang Fei-xue
Published: October 2023

Objective

To analyze the clinical and ultrasonic characteristics of breast sclerosing adenosis (SA) and invasive ductal carcinoma (IDC), and construct a predictive nomogram for SA.

Materials and methods

A total of 865 patients were recruited at the Second Hospital of Shandong University from January 2016 to November 2022. All patients underwent routine breast ultrasound examinations before surgery, and the diagnosis was confirmed by histopathological examination following the operation. Ultrasonic features were recorded using the Breast Imaging Data and Reporting System (BI-RADS). Of the 865 patients, 203 (252 nodules) were diagnosed as SA and 662 (731 nodules) as IDC. They were randomly divided into a training set and a validation set at a ratio of 6:4. Lastly, the difference in clinical characteristics and ultrasonic features were comparatively analyzed.

Result

There was a statistically significant difference in multiple clinical and ultrasonic features between SA and IDC (P<0.05). As age and lesion size increased, the probability of SA significantly decreased, with a cut-off value of 36 years old and 10 mm, respectively. In the logistic regression analysis of the training set, age, nodule size, menopausal status, clinical symptoms, palpability of lesions, margins, internal echo, color Doppler flow imaging (CDFI) grading, and resistance index (RI) were statistically significant (P<0.05). These indicators were included in the static and dynamic nomogram model, which showed high predictive performance, calibration and clinical value in both the training and validation sets.

Conclusion

SA should be suspected in asymptomatic young women, especially those younger than 36 years of age, who present with small-size lesions (especially less than 10 mm) with distinct margins, homogeneous internal echo, and lack of blood supply. The nomogram model can provide a more convenient tool for clinicians.
Identification of biomarker candidates for filarial parasite infections by analysis of extracellular vesicles
Authors: Yates Devyn , Di Maggio Lucia S. , Rosa Bruce A. , Sprung Robert W. , Erdmann-Gilmore Petra , Townsend R. Reid , Budge Philip J. , Kamgno Joseph , Mitreva Makedonka , Weil Gary J. , Fischer Peter U.
Published: October 2023

Background

Improved diagnostic tools are needed for detecting active filarial infections in humans. Tests are available that detect adult W. bancrofti circulating filarial antigen, but there are no sensitive and specific biomarker tests for brugian filariasis or loiasis. Here we explored whether extracellular vesicles released by filarial parasites contain diagnostic biomarker candidates.

Methods

Vesicles were isolated using VN96-affinity purification from supernatants of short-term in vitro cultured B. malayi microfilariae (Mf) and analyzed by mass spectrometry (Bruker timsTOF). Parasite-specific proteins were identified by bioinformatic analysis and a protein was called present if supported by ≥ 2 spectra. After validation with cultures parasites, this approach was then used to analyze vesicles isolated from plasma of animals infected with B. malayi and from humans with heavy Loa loa infections.

Results

Vesicles from Mf cultures contained more than 300 B. malayi proteins with high consistency across biological replicates. These included the known Mf excretory antigen BmR1 (AF225296). Over 150 B. malayi proteins were detected in vesicles isolated from plasma samples from two infected animals. Vesicles isolated from plasma from 10 persons with high L. loa Mf densities contained consistently 21 proteins, 9 of them were supported by at least 5 unique peptides and 7 with spectral counts above 10. The protein EN70_10600 (an orthologue of the B. malayi antigen BmR1, GenBank AF225296) was detected in all 10 samples with a total count of 91 spectra and a paralogue (EN70_10598) was detected in 6 samples with a total of 44 spectra.

Discussion

Extracellular vesicles released by filarial parasites in vitro and in vivo contain parasite proteins which can be reliably detected by mass spectrometry. This research provides the foundation to develop antigen detection assays to improve diagnosis of active filarial infections in humans.
Lipids: plant biology’s slippery superheroes
Authors: Moreau Hortense , Bayer Emmanuelle M.
Published: October 2023

Lipids are not only structural elements that make up biological membranes, they also play a crucial role in functionalizing these membranes. Through their ability to modulate membrane physical properties, to act as sensors and signaling molecules, and to interact with proteins to influence their subcellular localization and activity, lipids contribute the intricate workings of plant cells. The enrichment of specific lipids within distinct subcellular compartments aids to the establishment of membranes unique identity and properties. Lipids are major regulators of many cellular processes including cell signaling, cell division, cell polarity, membrane trafficking, intra- and intercellular communication, cell growth, and responses to environmental stress. In fact, the immense diversity of lipid species provides plant cells with an extensive arsenal of tools to establish distinctive biochemical identities within their membranes. In this review, we present an overview of plant membrane lipids, emphasizing their role in environmental stress response by highlighting recent advancements in the field.
A classification method of stress in plants using unsupervised learning algorithm and chlorophyll fluorescence technology
Authors: Lu Miao , Gao Pan , Hu Jin , Hou Junying , Wang Dong
Published: October 2023

Introduction

Chilling injury is one of the most common meteorological disasters affecting cucumber production. For implementing remedial measures as soon as possible to minimize production loss, a timely and precise assessment of chilling injury is crucial.

Methods

To evaluate the possibility of detecting cucumber chilling injury using chlorophyll fluorescence (ChlF) technology, we investigated the continuous changes in ChlF parameters under various low-temperature conditions and created the criteria for evaluating chilling injury. The ChlF induction curves were first collected before low-temperature as unstressed samples and daily 1 to 5 days after low-temperature as chilling injury samples. Principal component analysis was employed to investigate the public information on ChlF parameters and evaluate the differences between samples with different degrees of chilling injury. The parameters (Fv/Fm, Y(NO), qP, and Fo) accounted for a large proportion in the principal components and could characterize chilling injury. Uniform manifold approximation and projection method was employed to extract new features (Feature 1, Feature 2, Feature 3, and Feature 4) from ChlF parameters for subsequent classification model. Taking four features as input, a classification model based on the Fuzzy C-means clustering algorithm was constructed in order to identify the chilling injury classes of cucumber seedlings. The cucumber seedlings with different chilling injury classes were analyzed for ChlF images, rapid light curves, and malondialdehyde content.

Results and discussion

The results demonstrated that the variations in these indicators among the different chilling injury classes supported the validity of the classification model. Our findings provide a better understanding of the relationship between ChlF parameters and the impact of low-temperature treatment on cucumber seedlings. This finding offers an additional perspective that can be used to evaluate the responses and damage that plants experience under stress.
Exploring the relationships among safety leadership, safety climate, psychological contract of safety, risk perception, safety compliance, and safety outcomes
Authors: Omidi Leila , Karimi Hossein , Pilbeam Colin , Mousavi Saeid , Moradi Gholamreza
Published: October 2023

Background

Recently, two types of safety compliance behaviors including deep compliance and surface compliance were differentiated. The current study aimed to investigate the relationships among safety leadership, safety climate, psychological contract of safety (PCS), risk perception, and deep compliance and surface compliance behavior of workers. In addition, the effects of both deep and surface compliance on safety outcomes were considered.

Methods

Workers’ perceptions in terms of safety leadership, safety climate, PCS, risk perception, deep compliance, and surface compliance were measured by appropriate questionnaires. Three questions were asked to measure undesired safety outcomes. Structural equation modeling and correlation analysis were applied to examine the research model and relationships among variables.

Results and discussion

The results of the current study showed that deep compliance was positively predicted by safety leadership, safety climate, and PCS and negatively predicted by risk perception. Surface compliance was positively predicted by safety leadership and safety climate and negatively predicted by risk perception. Surface compliance is not significantly predicted by PCS. With regard to the adverse safety outcomes, the results showed that both deep and surface compliance were negatively associated with adverse safety outcomes, however, deep compliance had a stronger negative correlation with adverse safety outcomes than surface compliance.
Artificial water channels-embedded PVDF membranes for direct contact membrane distillation and ultrafiltration
Authors: Nursiah Kelvinraj , Musteata Valentina-Elena , Cerneaux Sophie , Barboiu Mihail
Published: October 2023

Innovative self-supported flat-sheet polyvinylidene fluoride (PVDF) membranes were developed incorporating amphiphilic I-quartet Artificial Water Channels (AWCs) and applied for membrane distillation (MD) and Dyes Ultrafiltration (UF). The presence of AWCs was aimed to increase the amount of water within hydrophobic PVDF pores increasing water permeability and preserving high selectivity and consequently to improve the MD and dyes UF performances. We explored novel strategies in which water channels structures contribute to water cluster stabilization and the increase of water (vapors or liquid) within hydrophobic pore structures. With this novel strategy in mind, three PVDF polymer grades with different molecular weights as well as the variation of their mass concentration as well as of AWCs were studied to shed in light their influence on the water permeability using a dead-end filtration setting. An enhanced water permeability of 75.3 L.m−2.h−1.bar−1 was attained for the PVDF-AWC hybrid membrane prepared using 16 wt% PVDF (530,000 g/mol) and 0.05 wt% AWCs when compared with a reference membrane with a water permeability of 30.6 L.m−2.h−1.bar−1. The MD performances of both membranes were assessed using a 35 g/L NaCl aqueous solution to yield a salt rejection of 95.3% and 85.2%, respectively. Furthermore, both the reference and the PVDF-AWC membranes showed improved separation performance in terms of rejection efficiency and dye permeability for binary dyes mixture as compared to single dyes. Among all the tested membranes, while methylene blue was completely removed in both cases, the 14 wt% PVDF membrane incorporating 0.075 wt% AWC showed a methyl orange rejection efficiency of up to 99.8% compared to 98.4% for its reference membrane. This hybrid membrane also displayed an almost doubled filtered dye feed permeability of 84 L.m−2.h−1.bar−1, compared to 40 L.m-2.h−1.bar−1 for its 14 wt% PVDF reference membrane.
The association of host genes with specific sexually transmitted infections
Authors: Bovungana Qhama , Arumugam Thilona , Ramsuran Veron
Published: October 2023

Sexually transmitted infections (STIs) are hazardous to human health worldwide. STIs have a direct influence on sexual and reproductive health and can increase the chances of HIV. Globally, more than 1 million STIs are acquired every day and the majority are asymptomatic. Approximately, 374 million cases of STIs have been reported annually. The most prevalent STIs include chlamydia, gonorrhoea, syphilis, and trichomoniasis. These STIs are caused by Chlamydia trachomatis, Neisseria gonorrhoeae, Treponema pallidum and Trichomonas vaginalis. The major factor that contributes to the susceptibility and prognosis of infectious diseases is genetic variation. Host genes play a huge role in STIs and immune response. The production of host factors is stimulated by a variety of bacteria, viruses and parasites and the host factors can play a role in increasing host vulnerability to infection and pathogen persistence. Genetic variation or polymorphisms within certain host genes can influence the course of pathogen infection and disease progression. Polymorphisms can contribute to changes in gene expression and or changes in the protein structure. which may either contribute to/or protect against infection. This review discusses the role of host genes in influencing the susceptibility of the most prevalent STIs caused by Chlamydia trachomatis, Trichomonas vaginalis, Treponema pallidum and Neisseria gonorrhoeae. We evaluate polymorphisms associated pathogen recognition signalling pathway of these diseases. These polymorphisms may be used as biomarkers to infer risk to specific STIs.