bluuebunny/crossref_metadata_2025 huggingface dataset:
Novel use of balloon-tipped bronchial blockers to occlude neonatal tracheoesophageal fistula: a case series
Authors: Sheng Bo , Zhong Lin , Du Bin
Published: January 2022

Background

Management of the airway and ventilation in neonates with a tracheoesophageal fistula (TEF) remains a significant challenge. The routine method of intubation involves placement of the tracheal tube tip beyond the fistula opening followed by isolation of the fistula from ventilation using the inflated cuff. When the fistula opening is close to the carina or below the level of the carina, the traditional technique is not suitable for adequate ventilation. Moreover, this method fails to prevent gastric insufflation.

Case presentation

We herein report a series of 10 newborns with TEFs (1,090–3,080 g) who underwent bronchoscopic insertion of a 5-Fr balloon-tipped bronchial blocker (BTBB) for temporary occlusion of the fistula. In seven newborns, placement of the BTBB was easily and quickly achieved with no incorrect placements. In addition, we successfully utilized the inner hollow cavity of the BTBB for gastric decompression in six neonates with severe gastric distension. However, three failed placements occurred in premature infants (<2,000 g) because the narrow cricoid cavity was too small to accommodate a 2.8-mm fiberoptic bronchoscope and a BTBB. The procedure was well tolerated by all infants, and no significant adverse events occurred.

Conclusions

Our findings illustrate that BTBBs can provide durable blockage of the fistula opening and should be considered as a treatment modality for infants with large carinal TEFs. Moreover, BTBB placement is neither arduous nor time-consuming. The hollow center, small round balloon, and 30-degree angled tip of the BTBB make this device feasible for clinical application, especially for neonates with severe gastrointestinal distension.
Analysis of E-mental health research: mapping the relationship between information technology and mental healthcare
Authors: Timakum Tatsawan , Xie Qing , Song Min
Published: January 2022

Background

E-mental healthcare is the convergence of digital technologies with mental health services. It has been developed to fill a gap in healthcare for people who need mental wellbeing support that may not otherwise receive psychological treatment. With an increasing number of e-mental healthcare and research, this study aimed to investigate the trends of an e-mental health research field that integrates interdisciplinary fields and to examine the information technologies is being used in mental healthcare. To achieve the research objectives, bibliometric analysis, information extraction, and network analysis were applied to analyze e-mental health research data.

Methods

E-mental health research data were obtained from 3663 bibliographic records from the Web of Science (WoS) and 3172 full-text articles from PubMed Central (PMC). The text mining techniques used for this study included bibliometric analysis, information extraction, and visualization.

Results

The e-mental health research topic trends primarily involved e-health care services and medical informatics research. The clusters of research comprised 16 clusters, which refer to mental sickness, e-health, diseases, information technology (IT), and self-management. The information extraction analysis revealed a triple relation with IT and biomedical domains. Betweenness centrality was used as a measure of network graph centrality, based on the shortest path to rank the important entities and triple relation; nodes with higher betweenness centrality had greater control over the network because more information passes through that node. The IT entity-relations of “mobile” had the highest score at 0.043466. The top pairs were related to depression, mobile health, and text message.

Conclusions

E-mental related publications were associated with various research fields, such as nursing, psychology, medical informatics, computer science, telecommunication, and healthcare innovation. We found that trends in e-mental health research are continually rising. These trends were related to the internet of things (IoT) and mobile applications (Apps), which were applied for mental healthcare services. Moreover, producing AI and machine learning for e-mental healthcare were being studied. This work supports the appropriate approaches and methods of e-mental health research that can help the researcher to identify important themes and choose the best fit with their own survey work.
Femoral neck fracture: the reliability of radiologic classifications
Authors: Cazzato Gianpiero , Oliva Maria Serena , Masci Giulia , Vitiello Raffaele , Smimmo Alessandro , Matrangolo Maria Rosaria , Palmacci Osvaldo , D’Adamio Stefano , Ziranu Antonio
Published: January 2022

Background

Femoral neck fractures (FNF) are one of the most common injury in the elderly. A valid radiographic classification system is mandatory to perform the correct treatment and to allow surgeons to facilitate communication. This study aims to evaluate reliability of 2018 AO/OTA Classification, AO/OTA simplified and Garden classification.

Methods

Six Orthopaedic surgeons, divided in three groups based on trauma experience, evaluated 150 blinded antero-posterior and latero-lateral radiography of FNF using Garden classification, 2018 AO/OTA and simplified AO/OTA classification. One month later, the radiographs were renumbered and then each observer performed a second evaluation of the radiographs. The Kappa statistical analysis was used to determine the reliability of the classifications. Cohen’s Kappa was calculated to determine intra and inter observer reliability. Fleiss’ Kappa was used to determine multi-rater agreement.

Results

The k values of interobserver reliability for Garden classification was from 0,28 to 0,73 with an average of 0,49. AO classification showed reliability from 0,2 to 0,42, with average of 0,30. Simplified AO/OTA classification showed a reliability from 0,38 to 0,58 with an average of 0,48.

The values of intra observer reliability for Garden classification was from 0,48 to 0,79 with an average of 0,63. AO classification showed reliability from 0,2 to 0,64 with an average of 0,5. Simplified AO/OTA classification showed a reliability from 0,4 to 0,75 with an average of 0,61.

Conclusion

The revised 2018 AO/OTA classification simplified the previous classification of intracapsular fracture but remain unreliable with only fair interobserver reliability. The simplified AO/OTA classification show a reliability similar to Garden classification, with a moderate interobserver reliability. The experience of the surgeons seems not to improve reliability. No classification has been shown to be superior in terms of reliability.
Genome-wide annotation of protein-coding genes in pig
Authors: Karlsson Max , Sjöstedt Evelina , Oksvold Per , Sivertsson Åsa , Huang Jinrong , Álvez María Bueno , Arif Muhammad , Li Xiangyu , Lin Lin , Yu Jiaying , Ma Tao , Xu Fengping , Han Peng , Jiang Hui , Mardinoglu Adil , Zhang Cheng , von Feilitzen Kalle , Xu Xun , Wang Jian , Yang Huanming , Bolund Lars , Zhong Wen , Fagerberg Linn , Lindskog Cecilia , Pontén Fredrik , Mulder Jan , Luo Yonglun , Uhlen Mathias
Published: January 2022

Background

There is a need for functional genome-wide annotation of the protein-coding genes to get a deeper understanding of mammalian biology. Here, a new annotation strategy is introduced based on dimensionality reduction and density-based clustering of whole-body co-expression patterns. This strategy has been used to explore the gene expression landscape in pig, and we present a whole-body map of all protein-coding genes in all major pig tissues and organs.

Results

An open-access pig expression map (www.rnaatlas.org) is presented based on the expression of 350 samples across 98 well-defined pig tissues divided into 44 tissue groups. A new UMAP-based classification scheme is introduced, in which all protein-coding genes are stratified into tissue expression clusters based on body-wide expression profiles. The distribution and tissue specificity of all 22,342 protein-coding pig genes are presented.

Conclusions

Here, we present a new genome-wide annotation strategy based on dimensionality reduction and density-based clustering. A genome-wide resource of the transcriptome map across all major tissues and organs in pig is presented, and the data is available as an open-access resource (www.rnaatlas.org), including a comparison to the expression of human orthologs.
Dichotomy between the humoral and cellular responses elicited by mRNA and adenoviral vector vaccines against SARS-CoV-2
Authors: Ukey Rahul , Bruiners Natalie , Mishra Hridesh , Mishra Pankaj K. , McCloskey Deborah , Onyuka Alberta , Chen Fei , Pinter Abraham , Weiskopf Daniela , Sette Alessandro , Roy Jason , Gaur Sunanda , Gennaro Maria Laura
Published: January 2022

Background

Protection from severe disease and hospitalization by SARS-CoV-2 vaccination has been amply demonstrated by real-world data. However, the rapidly evolving pandemic raises new concerns. One pertains efficacy of adenoviral vector-based vaccines, particularly the single-dose Ad26.COV2.S, relative to mRNA vaccines.

Main body

We investigated the immunogenicity of Ad26.COV2.S and mRNA vaccines in 33 subjects vaccinated with either vaccine class 5 months earlier on average. After controlling for the time since vaccination, Spike-binding antibody and neutralizing antibody levels were higher in the mRNA-vaccinated subjects, while no significant differences in antigen-specific B cell and T cell responses were observed between the two groups.

Conclusions

A dichotomy exists between the humoral and cellular responses elicited by the two vaccine classes. Testing only for humoral responses to compare the durability of SARS-CoV-2 vaccine-induced responses, as typically performed for public health and research purposes, is insufficient.
A UCMPs@MIL-100 based thermo-sensitive molecularly imprinted fluorescence sensor for effective detection of β-lactoglobulin allergen in milk products
Authors: Hong Liping , Pan Mingfei , Yang Xiao , Xie Xiaoqian , Liu Kaixin , Yang Jingying , Wang Shan , Wang Shuo
Published: January 2022

In this study, a thermo-sensitive molecularly imprinted fluorescence sensor was developed for the specific detection of β-Lactoglobulin (β-LG) allergen in milk products. The metal–organic frameworks (MIL-100) with a high specific surface area was coated on the surface of upconversion micro-particles (UCMPs). As the core, an imprinted polymer layer allowing for swelling and shrinking with response to temperature was prepared, which exhibited high adsorption and mass transfer capabilities for β-LG allergen. The fluorescence intensity of UCMPs@MIL-100@MIP decreased linearly with the concentration of β-LG in the range of 0.1–0.8 mg mL−1, and the limit of detection was 0.043 mg mL−1. The imprinting factor reached 3.415, which indicated that excellent specificity of the UCMPs@MIL-100@MIP for β-LG allergen. In the analysis of β-LG allergen in actual milk samples, the proposed UCMPs@MIL-100@MIP fluorescence sensor produced reliable and accurate results (recovery: 86.0–98.4%, RSD: 2.8–6.8%), closely related to the results of standard HPLC method (correlation coefficient: 0.9949), indicating that its feasibility in the detection of β-LG allergen.

Graphical Abstract
Balancing social and economic factors - explorative qualitative analysis of working conditions of supervisors in German social firms
Authors: Kordsmeyer Ann-Christin , Efimov Ilona , Lengen Julia Christine , Flothow Annegret , Nienhaus Albert , Harth Volker , Mache Stefanie
Published: January 2022

Background

Social firms are companies on the general labour market which provide employment to people with severe disabilities. In this setting different job resources are offered for its employees, including social support or flexibility in terms of working hours, tasks or pace of work. However, to date, only limited evidence exists on the work and health situation of supervisors in social firms. Therefore, the study aims to explore job demands and resources of supervisors in social firms to increase knowledge in a little researched field and to develop recommendations for action on workplace health promotion.

Methods

Sixteen semi-structured telephone interviews were conducted with supervisors of social firms in the North of Germany within July and November 2020. Different sectors such as gastronomy or cleaning services as well as employment opportunities for people with different types of disabilities were included. The interviews were recorded, transcribed verbatim and analysed following the qualitative content analysis according to Mayring (deductive-inductive approach).

Results

Overall, a heterogeneous composition was aimed for in terms of age and gender distribution (68.75% were male and between 32 and 60 years old). Supervisors reported various job demands in social firms, including for instance emotional demands, conflicts between social and economic objectives, conflict management, exposure to heat, heavy lifting or constant standing. In contrast, a high meaning of work, possibilities in shaping the structure of the social firm, social support of colleagues or the management and the provision of an ergonomic work environment were highlighted as job resources. Further person-related demands (e.g. own expectations) and resources (e.g. patience) were underlined as well.

Conclusion

First exploratory insights were provided with reference to job demands and resources for supervisors in social firms. The overview on working conditions underlines the significance of a supportive work environment taking structural and behavioural-related implications into account to reduce demands and strengthen resources. Further interventional research is needed regarding the development, testing and evaluation of workplace health promotion interventions in social firms.
Non-criteria manifestations in primary antiphospholipid syndrome: a French multicenter retrospective cohort study
Authors: Guédon Alexis F. , Catano Jennifer , Ricard Laure , Laurent Charlotte , de Moreuil Claire , Urbanski Geoffrey , Deriaz Sophie , Gerotziafas Grigorios , Elalamy Ismail , Audemard Alexandra , Chasset Francois , Alamowitch Sonia , Sellam Jérémie , Maillot François , Boffa Jean Jacques , Cohen Ariel , Abisror Noémie , Fain Olivier , Mekinian Arsène
Published: January 2022

Background

From this retrospective study, we aimed to (1) describe the prevalence and characteristics of non-criteria features in primary antiphospholipid syndrome (p-APS) and (2) determine their prognostic value.

Methods

This retrospective French multicenter cohort study included all patients diagnosed with p-APS (Sydney criteria) between January 2012 and January 2019. We used Kaplan-Meier and adjusted Cox proportional hazards models to compare the incidence of relapse in p-APS with and without non-criteria manifestations.

Results

One hundred and seventy-nine patients with p-APS were included during the study time, with a median age of 52.50 years [39.0; 65.25] and mainly women (n= 112; 62.6%). Among them, forty-three patients (24.0%) presented at least one non-criteria manifestation during the follow-up: autoimmune cytopenias (n= 17; 39.5%), Libman Sachs endocarditis (n= 5; 11.6%), APS nephropathy (n= 4; 9.3%), livedo reticularis (n= 8; 18.6%), and neurological manifestations (n= 12; 27.9%). In comparison to p-APS without any non-criteria manifestations (n= 136), p-APS with non-criteria features had more arterial thrombosis (n= 24; 55.8% vsn= 48; 35.3%;p= 0.027) and more frequent pre-eclampsia (n= 6; 14.3% vsn= 4; 3.1%;p= 0.02). The prevalence of triple positivity was significantly increased in patients with non-criteria features (n= 20; 47.6% vsn= 25; 19.8%;p= 0.001). Patients with p-APS and non-criteria manifestations (n= 43) received significantly more additional therapies combined with vitamin K antagonists and/or antiaggregants. Catastrophic APS (CAPS) tended to be more frequent in p-APS with non-criteria features (n= 2; 5.1% vs none;p= 0.074).

The p-APS with non-criteria manifestations had significantly increased rates of relapse (n= 20; 58.8% vs 33; 33.7%;p= 0.018) in bivariate analysis, but in survival analyses, the hazard ratio (HR) of relapse was not significantly different between the two groups (HR at 1.34 [0.67; 2.68];p= 0.40).

Conclusions

The presence of non-criteria features is important to consider, as they are associated with particular clinical and laboratory profiles, increased risk of relapse, and need for additional therapies. Prospective studies are necessary to better stratify the prognosis and the management of p-APS.
The role of TolA, TolB, and TolR in cell morphology, OMVs production, and virulence of Salmonella Choleraesuis
Authors: Li Quan , Li Zheng , Fei Xia , Tian Yichen , Zhou Guodong , Hu Yuhan , Wang Shifeng , Shi Huoying
Published: January 2022

The Tol–Pal system of Gram-negative bacteria is necessary for maintaining outer membrane integrity. It is a multiprotein complex of five envelope proteins, TolQ, TolR, TolA, TolB, and Pal. These proteins were first investigated in E. coli, and subsequently been identified in many other bacterial genera. However, the function of the Tol–Pal system in Salmonella Choleraesuis pathogenesis is still unclear. Here, we reported the role of three of these proteins in the phenotype and biology of S. Choleraesuis. We found that mutations in tolA, tolB, and tolR caused severe damage to the cell wall, which was supported by observing the microstructure of spherical forms, long chains, flagella defects, and membrane blebbing. We confirmed that all the mutants significantly decreased S. Choleraesuis survival when exposed to sodium deoxycholate and exhibited a high sensitivity to vancomycin, which may be explained by the disruption of envelope integrity. In addition, tolA, tolB, and tolR mutants displayed attenuated virulence in a mouse infection model. This could be interpreted as a series of defective phenotypes in the mutants, such as severe defects in envelope integrity, growth, and motility. Further investigation showed that all the genes participate in outer membrane vesicles (OMVs) biogenesis. Interestingly, immunization with OMVs from ΔtolB efficiently enhanced murine viability in contrast to OMVs from the wild-type S. Choleraesuis, suggesting its potential use in vaccination strategies. Collectively, this study provides an insight into the biological role of the S. Choleraesuis Tol–Pal system.
Neuropsychological exponents for the driving ability in remitted bipolar patients
Authors: Joachimiak Piotr , Jaracz Krystyna , Jaracz Jan
Published: January 2022

Background

Bipolar disorder (BD) is associated with cognitive deficits regardless of the phase of the disease. Medications used in treatment are an additional factor that may affect cognitive performance. Poor cognitive performance can significantly affect a patient's ability to drive.

Aim of the study

This study aims to explore cognitive functions relevant for safe driving in the group of remitted bipolar patients.

Method

Patients with BD in remission (n = 33) and healthy volunteers (n = 32) were included. Selected psychometric tests for drivers were carried out using computer software: called Specialistic Diagnostic Platform (SPD): The Cross-over Test (COT) version with free tempo (COT-F) and tempo of 50 tasks per minute (COT-50) and the Signal Test (ST). Moreover, the following neuropsychological tests were used: Rey Auditory Verbal Learning Test (RAVLT), Stroop Color-Word Test (SCWT) part A and B, and Trail Making Test (TMT) version A and B.

Results

In comparison with healthy controls bipolar patients in remission had poorer outcomes for some cognitive parameters and longer reaction times in both tests for drivers and neuropsychological tests. Additionally, we found a significant correlation between the time of performance of neuropsychological tests and the time of psychometric tests for drivers.

Conclusion

Patients with BD performed worse in several cognitive domains assessed by tests for drivers and neuropsychological tasks. These deficits can affect the speed of the patient's motor reactions while driving.
Tsunami inundation characteristics along the Japan Sea coastline: effect of dunes, breakwaters, and rivers
Authors: Yamanaka Yusuke , Shimozono Takenori
Published: January 2022

For Japanese coastal communities along the Japan Sea, where the risk of earthquake-induced tsunamis is deemed lower than that along the Pacific Ocean, tsunami disaster mitigation strategies have not been sufficiently developed. This study estimated the tsunami inundation characteristics for three major Japanese coastal cities along the Japan Sea. Based on tsunami simulations for representative coastal areas, we aimed to identify common vulnerabilities, which included those suggested by previous studies and local governments, to develop disaster mitigation strategies. Comprehensive simulations for tsunami propagation and inundation were performed for coastal areas in Akita City, Sakata City, and Niigata City based on earthquake and tsunami source scenarios developed by the Japanese Government for the Japan Sea area. To demonstrate the effectiveness of the estimated inundation characteristics, tsunami inundation was simulated for each coastal area under varying tsunami source conditions (including excessive tsunami magnitude) with a high resolution of the topography and infrastructure. Natural sand dunes, ports with man-made breakwaters, and rivers with levees are typical topographical features found in urbanized and densely populated areas along the Japan Sea coastline. According to the simulated results, the dunes and port breakwaters contribute significantly to decreasing the total amount of tsunami inundation for the areas behind them. However, the presence of the breakwaters also increases the tsunami heights in the areas immediately beyond the ports. Additionally, even the areas protected by the dunes might be exposed to an inundation risk in which the tsunamis invade a river channel, causing it to overflow. These findings were common to the coastal areas, and could be generally applicable to tsunami inundation for all coastal areas along the Japan Sea. Based on these results, the enhancement of existing infrastructure such as breakwaters and river levees should be the priority measure within tsunami risk-mitigation strategies; these, combined with natural resources such as dunes, can effectively mitigate tsunami disasters.

Graphical Abstract
Cross-species virus transmission and its pandemic potential
Authors: Choudhury Priyanka Ray , Saha Tapoja , Goel Sachin , Shah Janvi Manish , Ganjewala Deepak
Published: January 2022

Background

The majority of pandemics are known to be a result of either bacteria or viruses out of which viruses seem to be an entity of growing concern due to the sheer number of yet unidentified and potentially threatening viruses, their ability to quickly evolve and transform, their ability to transfer and change from one host organism to another and the difficulty in creating safe vaccines on time.

Main body

The present review attempts to bring forth the potential risks, prevention and its impact on the global society in terms of sociological and economic parameters. Taking hindsight from previously as well as ongoing current viral epidemics, this article aims to draw a concrete correlation between these viruses in terms of their origin, spread and attempts to compare how much they can affect the population. The study also assesses the worst-case scenarios and the amount of preparedness, required to fight against such pandemics and compares the required amount of preparedness to the current precautions and measures by different governments all across the world.

Short conclusion

Learning from the current pandemic, we can implement certain measures to prevent the adverse effects of pandemics in the future and through severe preparedness can combat the challenges brought about by the pandemic.
Gut microbiota of the threatened takahē: biogeographic patterns and conservation implications
Authors: West Annie G. , DeLaunay Anne , Marsh Phil , Perry Elena K. , Jolly Megan , Gartrell Brett D. , Pas An , Digby Andrew , Taylor Michael W.
Published: January 2022

Background

The Aotearoa New Zealand takahē (Porphyrio hochstetteri), once thought to be extinct, is a nationally threatened flightless rail under intensive conservation management. While there has been previous research into disease-related microbes in takahē, little is known about the microbes present in the gastrointestinal tract. Given the importance of gut-associated microbes to herbivore nutrition and immunity, knowledge of these communities is likely to be of considerable conservation value. Here we examined the gut microbiotas of 57 takahē at eight separate locations across Aotearoa New Zealand.

Results

Faecal samples, taken as a proxy for the hindgut bacterial community, were subjected to 16S rRNA gene amplicon sequencing using Illumina MiSeq. Phylogenetic analysis of > 2200 amplicon sequence variants (ASVs) revealed nine main bacterial phyla (Acidobacteriota, Actinobacteriota, Bacteroidota, Campilobacterota, Firmicutes, Fusobacteriota, Planctomycetota, Proteobacteria, and Verrucomicrobiota) that accounted for the majority of sequence reads. Location was a significant effect (p value < 0.001, 9999 permutations) that accounted for 32% of the observed microbiota variation. One ASV, classified as Lactobacillus aviarius, was present in all samples at an average relative abundance of 17% (SD = 23.20). There was strong evidence (p = 0.002) for a difference in the abundance of the genus Lactobacillus between locations. A common commensal bacterium previously described in takahē, Campylobacter spp., was also detected in most faecal samples.

Conclusions

Location plays a pivotal role in the observed variation among takahē gut bacterial communities and is potentially due to factors such as supplemental feeding and medical treatment experienced by birds housed in captivity at one of the eight sampled sites. These data present a first glimpse of the previously unexplored takahē gut microbiota and provide a baseline for future microbiological studies and conservation efforts.
Modeling, analyzing and simulating the dynamics of Lassa fever in Nigeria
Authors: Ojo Mayowa M. , Goufo Emile Franc Doungmo
Published: January 2022

Lassa fever is an infectious and zoonotic disease with incidence ranging between a hundred to three hundred thousand cases, with approximately five thousand deaths reported yearly in West Africa. This disease has become endemic in the Lassa belt of Sub-Saharan Africa, thus increasing the health burden in these regions including Nigeria. A deterministic mathematical model is presented to study the dynamics of Lassa fever in Nigeria. The model describes the transmission between two interacting hosts, namely the human and rodent populations. Using the cumulative number of cases reported by the Nigerian Centre for Disease Control within the first week of January 2020 through the eleventh week in 2021, we performed the model fitting and parameterization using the nonlinear least square method. The reproduction number $${\mathcal {R}}_{0}$$

R
0

which measures the potential spread of Lassa fever in the population is used to investigate the local and global stability of the system. The result shows that the model system is locally and globally asymptomatically stable whenever $${\mathcal {R}}_{0}<1$$

R
0

<
1

, otherwise it is unstable. Furthermore, the endemic equilibrium stability is investigated and the criteria for the existence of the phenomenon of bifurcation is presented. We performed the sensitivity analysis of each reproduction number parameter and solutions of the developed model are derived through an iterative numerical technique, a six-stage fifth-order Runge–Kutta method. Numerical simulations of the total infected human population $$(E_{h}+I_{h})$$

(

E
h

+

I
h

)

under different numerical values (controlled parameters) are presented. The result from this study shows that combined controlled parameters made the total infected human population decline faster and thus reduces Lassa fever’s burden on the population.
In-silico analysis unravels the structural and functional consequences of non-synonymous SNPs in the human IL-10 gene
Authors: Das Shuvo Chandra , Rahman Md. Anisur , Das Gupta Shipan
Published: January 2022

Background

Interleukin-10 (IL-10) is an anti-inflammatory cytokine that affects different immune cells. It is also associated with the stimulation of the T and B cells for the production of antibodies. Several genetic polymorphisms in the IL-10 gene have been reported to cause or aggravate certain diseases like inflammatory bowel disease, rheumatoid arthritis, systemic sclerosis, asthma, etc. However, the disease susceptibility and abnormal function of the mutated IL-10 variants remain obscure.

Results

In this study, we used seven bioinformatics tools (SIFT, PROVEAN, PMut, PANTHER, PolyPhen-2, PHD-SNP, and SNPs&GO) to predict the disease susceptible non-synonymous SNPs (nsSNPs) of IL-10. Nine nsSNPs of IL-10 were predicted to be potentially deleterious: R42G, R45Q, F48L, E72G, M95T, A98D, R125S, Y155C, and I168T. Except two, all of the putative deleterious mutations are found in the highly conserved region of IL-10 protein structure, thus affecting the protein's stability. The 3-D structure of mutant proteins was modeled by project HOPE, and the protein–protein interactions were assessed with STRING. The predicted nsSNPs: R42Q, R45Q, F48L, E72G, and I168T are situated in the binding site region of the IL-10R1 receptor. Disruption of binding affinity with its receptor leads to deregulation of the JAK-STAT pathway and results in enhanced inflammation that imbalance in cellular signaling. Finally, Kaplan–Meier Plotter analysis displayed that deregulation of IL-10 expression affects gastric and ovarian cancer patients' survival rate. Thus, IL-10 could be useful as a potential prognostic marker gene for some cancers.

Conclusion

This study has determined the deleterious nsSNPs of IL-10 that might contribute to the malfunction of IL-10 protein and ultimately lead to the IL-10 associated diseases.
Comparison of radiation dose and its correlates between coronary computed tomography angiography and invasive coronary angiography in Northeastern Thailand
Authors: Aupongkaroon Phatraporn , Makarawate Pattarapong , Chaosuwannakit Narumol
Published: January 2022

Background

The number of coronary computed tomography angiography (CCTA) exams is steadily growing. A novel computed tomography (CT) system has been developed to increase image quality while lowering patient radiation. The radiation dose attributed to CCTA has received considerable attention, whereas the dose associated with invasive catheter angiography (ICA) has received less. This study aims to investigate the radiation exposure of CCTA in patients and compare it to ICA.

Results

The mean effective dose of CCTA was 2.88 ± 0.85 mSv which was significantly lower than the mean effective dose of ICA (5.61 ± 0.55 mSv), p < 0.0001. The effective dose of CCTA correlated with the weight, height, and BMI, while the effective dose of ICA was associated with patient weight and BMI. The radiation exposure from CCTA has been considerably reduced over the last ten years by almost 2.5 folds. The mean radiation dose from the newer generation CT used in 2019 was significantly lower than that of the single-source CT in 2010 (2.88 ± 0.85 mSv vs. 7.15 ± 3.4 mSv, p < 0.001).

Conclusions

CCTA allows evaluation of CAD with a significantly less effective radiation dose to patients than diagnostic ICA. There was a significant decrease in radiation dose from CCTA over time. Regular measurement of patient doses is an essential step to optimize exposure. It makes operators aware of their performance and allows comparisons with generally accepted practices.
Nella Larsen’sQuicksand, Recalcitrant Subjects, and Wrong Feeling
Authors: Priest Madison
Published: January 2022

Helga Crane, the heroine of Nella Larsen’s critically acclaimed 1928 novel, Quicksand, is a maddening protagonist. Hysterical, reactive, impulsive, and compulsive, she seems constitutionally incapable of finding any sort of happiness. In accounting for Helga’s frustrating and inexplicable choices, critics tend to blame either Helga’s psyche or her environment. This essay offers an alternative approach, one that troubles the sharp distinctions between interior and exterior on which these readings implicitly rely by arguing that Helga and recalcitrant subjects like her exhibit “wrong feeling.” Wrong feeling is a peculiarly twentieth-century phenomenon—an enactment of the modernist allergy to sentiment that nonetheless takes up modernism’s key tropes. Manifesting as affective overflowing, it has no discernible locus in either self or world and yields a series of repetitive, frustrating, and ineffectual choices. In disrupting the divide between interior and exterior, wrong feeling provides an unsettling critique of the world that undoes Helga, even as it implicates Helga in her own undoing. In the end, this essay tells a story of punishment—of the iterative mechanisms and repercussions of feeling the wrong way, about the wrong things and for the wrong reasons—tracing the workings of wrong feeling within Larsen’s first novel and beckoning toward the ways the idea might help us recognize the import of recalcitrant subjectivity beyond Quicksand itself.
Physiotherapist beliefs and perspectives on virtual reality–supported rehabilitation for the assessment and management of musculoskeletal shoulder pain: a focus group study protocol
Authors: Brady Niamh , Lewis Jeremy , McCreesh Karen , Dejaco Beate , McVeigh Joseph G.
Published: January 2022

Shoulder pain accounts for a large proportion of musculoskeletal disorders and years lived with disability. Musculoskeletal shoulder pain is challenging to manage and while research evidence suggests that exercise should be a cornerstone of shoulder pain rehabilitation, the exact type and dosage of exercise is unclear. Adherence is a barrier to successful outcomes with exercise-based management of musculoskeletal pain, especially for those with co-morbidities, high pain levels and reported boredom associated with competing prescribed exercise. Virtual reality (VR) may offer an effective platform for rehabilitation of musculoskeletal shoulder pain. Virtual Reality has been shown to be effective for management of acute and chronic pain conditions, for delivering education around various health conditions, and for supporting rehabilitation of neurological conditions. Therefore, it is possible that VR may have a role in the delivery of exercise and education for individuals with musculoskeletal shoulder pain. VR intervention design should involve several steps and begin with establishing early acceptability from users as to the suitability of the technology in clinical practice as well as potential barriers and facilitators to using the technology successfully. This study will therefore aim to explore physiotherapists beliefs and perspectives of immersive VR as a platform for assessment and rehabilitation in patients with musculoskeletal shoulder pain. Further, this study will inform the development of a VR intervention for use in the rehabilitation of musculoskeletal shoulder pain. A series of online focus groups will be conducted with physiotherapists in Ireland using a qualitative descriptive approach to data analysis. A six-phase process of data analysis will be carried out to identify important patterns and themes within the data. The current study will be the first to explore clinician’s perspectives on the role of VR in musculoskeletal practice.
Leiu Heapost 85
Authors: Veldre Gudrun , Allmäe Raili
Published: January 2022

Leiu Heapost 85
ПРИМЕНЕНИЕ ИНФОРМАЦИОННЫХ ТЕХНОЛОГИЙ В СРЕДЕ ДИСТАНЦИОННОГО ФОРМАТА ОБУЧЕНИЯ У СТУДЕНТОВ МЕДИКО-БИОЛОГИЧЕСКОГО ПРОФИЛЯ
Authors: Mazhitova Marina V. , Uranova Valeria V. , Bliznyak Olga V.
Published: January 2022

Введение. Данная статья посвящена применению информационных технологий в среде дистанционного формата обучения у студентов медико-биологического профиля.
Цель исследования определила необходимость перехода на дистанционное обучение образовательных организаций и более активное применение информационных технологий в период распространения COVID-19.
Методы. Исследование и анализ проведены методом анкетирования в ходе социологического опроса и по данным, представленным в программно-методическом комплексе LiftUp. Полученные числовые данные обрабатывали методом математической статистики в Calc из офисного пакета OpenOffice.
Результаты. Результаты анкетирования позволили выявить, что с внедрением цифровых технологий увеличилась эффективность обучения студентов 3-5 курсов, поскольку они готовы к самостоятельной работе. Однако, студенты первого курса из-за отсутствия опыта работы в цифровой среде испытывают ряд сложностей.
Область применения результатов. Результаты исследования могут быть использованы в качестве статистических данных, позволяющих выявить особенности применения информационных технологий в среде дистанционного обучения в условиях распространения COVID-19.
Заключение. Было выявлено, что в условиях современного мира для увеличения эффективности образовательного процесса необходимо внедрять информационные технологии. Результаты анкетирования определили перспективность использования цифровой среды в процессе освоения образовательных программ. Повышение качества образования зависит от заинтересованности студентов. Поскольку дистанционный формат имеет ряд несовершенств, современная образовательная система нацелена на внедрение гибридного формата, который объединяет формы традиционного обучения и цифровых технологий.
ПЕДАГОГИЧЕСКАЯ РЕФЛЕКСИЯ УДОВЛЕТВОРЕННОСТИ ПЕРВОКУРСНИКОВ ОБРАЗОВАТЕЛЬНЫМ ПРОЦЕССОМ В МЕДИЦИНСКОМ УНИВЕРСИТЕТЕ
Authors: Zinkevich Elena R. , Klikunova Kseniia A.
Published: January 2022

Цель. Статья посвящена частным вопросам педагогической рефлексии, предполагающей аналитическую работу, иллюстрирующую отношение студентов 1 курса к организации и содержанию образовательного процесса в рамках изучения учебного предмета «Физика. Математика» в медицинском педиатрическом университете. Цель исследования сводилась к изучению удовлетворенности первокурсников образовательным процессом в университете.
Метод или методология проведения работы. Основой исследования стали личностно ориентированный и гуманистический подходы, образовательные традиции, обеспечивающие развитие критического мышления будущих врачей, ценностное отношение профессорско-преподавательского состава к смыслам педагогической деятельности, понимание необходимости педагогической рефлексии. Педагогические и математико-статистические методы позволили реализовать цель исследования.
Результаты. Обнаружены факторы, определяющие удовлетворенность первокурсников организацией и содержанием образовательного процесса в рамках изучения учебного предмета «Физика. Математика» в медицинском университете, средикоторых: форма обучения в вузе, гендерная принадлежность обучающихся и их суммарный балл единого государственного экзамена.
Область применения результатов. Данные, полученные в процессе исследования, обусловливают необходимость учета гендерных особенностей обучающихся в образовательном процессе, осознания преподавателями существующей зависимости между удовлетворенностью субъектов образовательным процессом и такими факторами как форма обучения, суммарный балл единого государственного экзамена. В настоящее время учреждения образования отнесены к сфере услуг, потребители которых могут предъявлять повышенные требования к качеству своего образования, за которое платят.
CPD route to wellbeing
Authors: Marsh Sian
Published: January 2022

The value of CPD as a tool for promoting staff wellbeing was one of the big learning points for the early years sector over the past year. We need to learn from that, says Sian Marsh of Best Practice Network.
Topical oxygen treatment relieves pain from hard-to-heal leg ulcers and improves healing: a case series
Authors: Jebril William , Nowak Marcela , Palin Lena , Nordgren Maria , Bachar-Wikstrom Etty , Wikstrom Jakob D
Published: January 2022

Managing painful hard-to-heal leg ulcers is challenging with current therapeutic options. Wounds are prone to being hypoxic, and the subsequent pain is often related to hypoxia. Hyperbaric oxygen therapy (HBOT) is used to treat hard-to-heal leg wounds by delivering 100% oxygen at a pressure 2–3 times higher than atmospheric pressure. Unfortunately, most patients cannot be offered HBOT because it is costly and needs to be applied at specialised centres. Therefore, topical continuous oxygen therapy (TCOT) is a novel alternative for continuous local oxygen delivery to wounds and is associated with improved wound healing; however, its effect on painful wounds is unknown. This retrospective study was conducted on 20 patients, of whom 17 had painful hard-to-heal leg ulcers. In 13 patients (76%) with painful ulcers, TCOT was associated with rapid and substantial pain alleviation. Also, eight (40%) of the patients' wounds healed entirely with TCOT. This study suggests that TCOT may represent a novel pain management device for hard-to-heal wounds.
High Fat Diet and Ethanol-Induced Fatty Liver Disease Mouse Model
Authors:   Research Group for Pharmaceutical Activities of Natural Products using Pharmaceutical Biotechnology (PANPB), Khon Kaen University, Khon Kaen 40002 Thailand, Sukkasem Nadta ,   Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002 Thailand, Chatuphonprasert Waranya ,   Research Group for Pharmaceutical Activities of Natural Products using Pharmaceutical Biotechnology (PANPB), Khon Kaen University, Khon Kaen 40002 Thailand,   Faculty of Medicine, Mahasarakham University, Maha Sarakham 44000 Thailand, Jarukamjorn Kanokwan ,   Research Group for Pharmaceutical Activities of Natural Products using Pharmaceutical Biotechnology (PANPB), Khon Kaen University, Khon Kaen 40002 Thailand,   Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002 Thailand
Published: January 2022

Abstract A diet high in fat and/or alcohol is a major cause of fatty liver disease (FLD), which is a common precursor to chronic liver disease. As the pathogenetic mechanisms of FLD remain unclear, an appropriate animal model is critical to problem solving in this field. This study aimed to develop an FLD mouse model using a diet high in fat and/or ethanol. Five-week-old female ICR mice were given free access to a high fat diet (HFD, 60 kcal % fat of total food), or daily intragastrically administered ethanol (E, 0.5 g/kg/day), or a combination of HFD and ethanol (HFD+E). Hepatic histology was observed with oil red O (ORO) staining. Hepatic triglyceride (TG) levels, antioxidant enzyme activities, and mRNA expression levels of metabolic, antioxidant, and inflammatory genes, i.e. peroxisome proliferator activated receptor-alpha and gamma (Ppar-α and Ppar-γ), sterol regulatory element binding protein-1 (Srebp-1), acetyl-CoA carboxylase (Acc), fatty acid synthase (Fas), fat cluster of differentiation (Cd-36), catalase (Cat), superoxide dismutase 1 and 2 (Sod1 and Sod2), glutathione peroxidase (Gpx), nuclear factor-kappa b (Nf-ĸb), tumor necrosis factor-alpha (Tnf-α), and monocyte chemoattractant protein-1 (Mcp-1) were determined. HFD+E induced FLD in mice by increasing hepatic TG levels and expression of Acc and Fas metabolic genes and Cd-36 and Mcp-1 inflammatory genes, while simultaneously reducing the activity and expression of antioxidant enzymes. These findings confirm that HFD+E is a potent regimen for FLD induction in mice. Keywords: High fat diet, Alcohol, Non-invasive, Antioxidation, Metabolic gene, Inflammatory gene
3D Multimedia Packaging Design Based on Agile Software Development and IoT Platform
Authors: Sang-aroon Patinya , Prongnuch Sethakarn , Sitjongsataporn Suchada
Published: January 2022

This paper aims to present the three-dimensional (3D) packaging structure design based on the agile software development and internet of things (IoT) platform, which an efficiency validation of IoT platform is required. The objective of this research is to investigate the model development of 3D multimedia-based packaging structure using the webserver design and agile software development applied in the design and implementation process. This model development methodology comprises how the IoT platform as the client-server architecture that is used to develop and support the orthographic projection techniques, information sheet, worksheet and multimedia presentation in the modern product design. The empirical results show the creative thinking of the group of young designers that can be achieved efficiently. The proposed model development can increase significantly the fundamental knowledge and interactive design ideas of young designers combining from the two-dimension to three-dimension packaging structure model.
CCGraMi: An Effective Method for Mining Frequent Subgraphs in a Single Large Graph
Authors: Nguyen Lam B. Q. , Zelinka Ivan , Diep Quoc Bao
Published: January 2022

In modern applications, large graphs are usually applied in the simulation and analysis of large complex systems such as social networks, computer networks, maps, traffic networks. Therefore, graph mining is also an interesting subject attracting many researchers. Among them, frequent subgraph mining in a single large graph is one of the most important branches of graph mining, it is defined as finding all subgraphs whose occurrences in a dataset are greater than or equal to a given frequency threshold. In which, the GraMi algorithm is considered the state of the art approach and many algorithms have been proposed to improve this algorithm. In 2020, the SoGraMi algorithm was proposed to optimize the GraMi algorithm and presented an outstanding performance in terms of runtime and storage space. In this paper, we propose a new algorithm to improve SoGraMi based on connected components, called CCGraMi (Connected Components GraMi). Our experiments on four real datasets (both directed and undirected) show that the proposed algorithm outperforms SoGraMi in terms of running time as well as memory requirements.
Versatile polarization manipulation in vanadium dioxide-integrated terahertz metamaterial
Authors: Lv Tingting , Li Yicheng , Qin Chunhua , Qu Jia , Lv Bo , Li Wenjia , Zhu Zheng , Li Yuxiang , Guan Chunying , Shi Jinhui
Published: January 2022

Broadband and switchable versatile polarization metamaterial is crucial in the applications of imaging, sensing and communication, especially in the terahertz frequency. Here, we investigated versatile polarization manipulation in a hybrid terahertz metamaterial with bilayer rectangular rods and a complementary vanadium dioxide (VO2) layer. The VO2 phase transition enables a flexible switching from dual-band asymmetric transmission to dual-band reflective half-wave plate. The full width half maximum (FWHM) bandwidths of dual-band asymmetric transmission are 0.77 and 0.21 THz, respectively. The polarization conversion ratio (PCR) of the reflective metamaterial is over 0.9 in the frequency ranges of 1.01-1.17 THz and 1.47-1.95 THz. Angular dependences of multiple polarization properties are studied. The proposed switchable polarization metamaterial is important to the development of multifunctional polarization devices and multichannel polarization detection.