bluuebunny/crossref_metadata_2025 huggingface dataset:
DESIGN OF TEMPERATURE REGULATOR OF A FISHPOND
Authors: Saleh Yusuf Bello
Published: July 2023

This research is about a digital temperature regulator in the fishpond. Which is a circuit through which the temperature of the fishpond is linearly controlled depending on the temperature of the water or the environment. This device makes use of a heating element, which goes ON/OFF when the temperature in the water becomes high/low or reaches a temperature higher or lower than the present value. The temperature sensor used is LM35 with a Negative Temperature Coefficient (NTC) in which its resistance falls as the operating temperature increases. The LM35 is a very sensitive device to heat, such as it can be used in a way that an increase in temperature will lead to a change in its resistance. Fishes survive in natural water under the very conditions it is assumed that natural waters have natural temperature regulators but in fishponds nevertheless. They find it difficult to survive in harsh weather conditions like the cool season (winter). The temperature will be controlled between 24°C – 30°C which is the optimum survival temperature of the fish. This is because, so as the temperature of the environment changes, so that of the water too by convection.
Study of hybrid passive-active micromixers under an acoustic field
Authors: Kharaji Zahra Ghorbani , Kalantar Vali , Bayareh Morteza
Published: July 2023

This paper presents a numerical investigation of the mixing performance of two hybrid passive active micromixers subjected to an acoustic field. The study employs the Generalized Lagrangian Mean (GLM) theory and the convection-diffusion equation to analyze concentration profiles within the micromixers, providing a comprehensive assessment of their performance. Perturbation theory is employed to solve the equations of zeroth-, first-, and second-order, resulting in the determination of the final flow velocity from the zeroth- and second-order solutions. Notably, the study incorporates a non-zero background laminar flow to explore the interaction between acoustic waves and a moving laminar flow. The findings reveal that while the presence of sharp-edge structures on the channel walls significantly enhances mixing quality, this improvement is not observed that much when rectangular structures.
Küresel Salgınlarda Blok Zinciri Tabanlı Akıllı Kontratların Kullanımı
Authors: BAYRAM Emre , YURTTAKAL Ahmet Haşim
Published: July 2023

2019 yılında ortaya çıkan ve tüm dünyayı her anlamda etkileyen Covid-19 pandemisi, binlerce insanın hayatını kaybetmesine, sağlık sistemlerinin çökmesine, ekonomik tedarik zincirlerinin kırılmasına sebep olmuştur. Bu süreçte, ülkeler arası veri paylaşım sistemlerinin tasarlanması çok gecikmiştir. Ülkelerin birbirleriyle entegre olmayan pandemi takip sistemlerinde, şeffaf olmayan uygulamaların yapılması ile birlikte iş daha da çıkmaza girerek ekonomik bir krize dönüşmüştür. Örneğin turizm gelirlerinden vazgeçmek istemeyen ve Covid-19 verilerini saklayan ülkelerin şeffaf olmamalarından dolayı pandemi süreci uzamıştır. Belirli bir süre sonunda uluslararası aşı kartları veya HES kodları oluşturulmuştur. Bu kodların kontrolü ve geçerlilik sürelerinin kontrol edilmesi çok uzun bir süre sonunda uluslararası bir formata sokulabilmiştir. Bu örnekler, pandemiler de veriye ulaşma hızının ne kadar önemli olduğunu ortaya koymaktadır.Blok zinciri teknolojisi güvenli, şeffaf ve hızlı bir şekilde veriye ulaşma konusunda çok önemli fırsatlar sunmaktadır. Akıllı kontrat teknolojisi ile otomatikleştirilmiş veri kayıt ve listeleme fonksiyonları oluşturulmaktadır. Zincire eklenen her düğümde (bilgisayarda), zincirin bir yedeği kayıtlı olduğu için son derece güvenli ve dağıtılmış, herkesin ulaşabilmesi sebebi ile de şeffaflık ilkesine son derece uygun olmaktadır.Blok zinciri ortamında, öncelikle bireyi kişisel bilgileri ile birlikte kayıt edip, daha sonrasında enfekte olma durumlarını ve aşı bilgilerini güncelleyebilmekteyiz. Böylelikle hızlı bir şekilde blok zinciri ortamına aktarılan veriler pandeminin ilerleme hızından daha hızlı bir şekilde işlenerek, problemlerin oluşmadan ortadan kaldırılmasına fayda sağlamakta ve ülkelerin birbirleriyle ilgili verilerini görmelerine olanak sağlamaktadır. Sadece ülkeler bazında değil ülke içinde de verilere ulaşıp, sağlık operasyonlarının daha verimli bir şekilde çalışmaları amaçlanmaktadır.
Beyin Dalgaları Kontrollü Maket Tekerlekli Sandalye
Authors: Fışkın Bayram , Göllü Mehmet , Zengin İsmail , Yüksel Emirhan , Varol İsmail , Tabakcıoğlu Mehmet Barış
Published: July 2023

Omurilik felci hastaları ve bazı kas hastaları hiçbir azasını kullanamamakta ve sürekli olarak tekerlekli sandalyeyle hareket etmeye mahkûm veya yatalak haldedir. Bu hastaların sosyal hayata bağlanabilmesi çok zordur. Refakatçı olmadan bu hastaların dışarıya çıkması veya ev içinde hareket etmesi mümkün değildir. Hastabakıcılar açısından düşünülürse de bu hastaların bakımı oldukça zordur. Her iki tarafın da yükünü azaltmak için; bu hastalara uygun, ihtiyaçlarına karşılık verebilen ve refakatçı olmadan hareket edebilmeyi sağlayan bir maket tekerlekli sandalye tasarlanmıştır. Bu bağlamda EEG sinyallerini ölçüp, meditasyon, dikkat ve göz kırpma verilerine çeviren ve bu bilgileri bluetooth ile tekerlekli sandalye üzerindeki kontrolcüye gönderebilen Neurosky Mindwave Mobil2 biyosensör kullanılmıştır. Biyosensörden gönderilen veriler Raspberry Pi kontrolcüsü tarafından alınmış ve yazılan programa göre maket tekerlekli sandalye hareket ettirilmiştir.
GPS Verisinin Alınamadığı Ortamlarda Otonom Görev Yapabilen Gemi İçin Sensör Kaynaştırma Algoritmaları Tasarımı, Gerçeklenmesi ve Testi
Authors: ÇELİK Caner , BECERİKLİ Yaşar
Published: July 2023

Günümüzde yapılan otonom araç çalışmalarında insansız deniz araçları da önemli yer tutmaktadır. Su üstü ve sualtı araçları seyirlerini gerçekleştirdikleri sırada bulundukları konum bilgisine ihtiyaç duyarlar. İlgili konum bilgisi araçların yönlerini tayin etmeleri konusunda yardımcı olur. Denizcilerin konum bildirme uygulamaları haricinde özellikle geçmişte kullandıkları bazı yöntemler vardır. Parakete Seyri, Pusula ile Yön Tayini, Yersel ve Göksel Seyir çalışmaları denizcilerin yön seçiminde hayli önemlidir. Çıkış noktaları, hızları ve konumu bilinen araçlar için, daha önce belirlenmiş lokasyonlar kerteriz alınarak sapmalar hesaplanabilir ve yön tayini yapılabilir. Otonom deniz araçlarında insan faktörü bulunmayacağından bu araçlarda yön tayini yapılabilmesi için GPS (Global Positioning System) vb. global konum belirleme sistemlerinin haricindeki yön belirleme çalışmalarını yapabilecek bir sisteme ihtiyaç duyulur. Bu sistem bizim çalışmamızda oluşturulmuş ve maket bir gemiye entegre edilmiştir. Bu bildiri kapsamında gemi başlangıç noktasında tanımlanan rotaya uygun olarak pusula ile yön tayini yapmış, seyir esnasında görüntü işlemiş, konum belirlemiş ve otonom bir şekilde limana aborda olmuştur. Test ortamında bulunan ve koordinatları bilinen adalar, şamandıralar, sahiller vb. sisteme eklenmiş ve bu noktalara gelindiğinde aracın bulunduğu konum bilgisi güncellenmiş ve rota bu koordinata uygun olarak yeniden değerlendirilmiştir. Gerçekleştirilen otonom gemi projesinde bu veriler işlenerek geminin konum bilgisi, rotası ve rota tutma kabiliyeti incelenmiş ve geliştirilmiştir. İlerleyen süreçte farklı ölçüm yöntemleri ve algoritmalar kullanılarak aralarındaki farklar incelenecek ve en optimum sonuca ulaşılan yöntem ile geliştirme sürecine devam edilecektir.
Design and Optimization of a Super Capacitor-Based High-Speed Charging System for Electric Vehicles
Authors: SUBAŞI Semih , AYDEMİR Gürkan
Published: July 2023

In this study, the design and optimization of high-speed charging systems for supercapacitor banks are investigated to meet the fast charging needs of electric vehicles. The limitations of current energy storage technologies and the requirements for fast charging of electric vehicles are addressed. While examining the advantages and energy storage potentials of supercapacitors, appropriate capacity, and voltage levels are determined in the design process to increase the charging speed. Additionally, power electronic components and control strategies are discussed to enhance the efficiency of the charging system. These components and strategies aim to optimize the fast charging process by enabling supercapacitors to be charged with maximum efficiency. The effectiveness and optimization potential of the design are evaluated and discussed through simulations conducted using the Matlab/Simulink software program. This article emphasizes the significance of supercapacitor-based high-speed charging systems in meeting the fast charging needs of electric vehicles and provides a foundation for future advancements. With the progress of electric vehicle technologies, supercapacitor-based charging systems aim to enable faster and more efficient charging, thereby further popularizing the use of electric vehicles.
Dynamic and Static Analysis of Deep Excavation of Poligon Station in İzmir Metro Line
Authors: Başarı Ender , Tuskan Yeşim
Published: July 2023

In this paper, the deep excavation stages of Poligon Station which is the located along the Üçyol–Fahrettin Altay subway line was modeled numerically. Although some parts of the metro line were constructed, some parts of metro line are currently under construction in İzmir. The Poligon Station is carried out with a surrounding diaphragm wall and top-down construction method. The foundation mat and four slabs of the station building are used as support elements. The excavation depth of the station is 18 meters. The soil properties of the numerical model were obtained engineering boreholes and laboratory tests. Lateral soil displacements were measured with inclinometers installed in the nearby soil layers. Displacements which were measured from inclinometers and obtained from numerical modeling were compared. Beside the static analysis, the Poligon station of the metro line was analyzed with a model earthquake. 16.12.1977 İzmir Earthquake (Ms=5.5, amax=0.21g) was used as a model earthquake for dynamic analyses. In addition, effects of changing the properties of the soil on computed results were numerically investigated using the software Plaxis.
Hrvatski demografski i migracijski nesklad
Authors: Šterc Stjepan
Published: July 2023

Demografski su trendovi i pokazatelji te brojni dosadašnji stručni i znanstveni objavljeni radovi jasno upozoravali na hrvatsku demografsku stvarnost, koju obilježava demografski i migracijski nesklad vidljiv praktički u cijelom hrvatskom društvu i prostoru. Potvrda je stigla i s objavom službenih rezultata popisa stanovništva 2021. godine i razinom depopulacije, ali posebno i s objavom prirodnog pada stanovništva posljednje tri godine. Provedena anketna ispitivanja javnog mijenja 2019. i 2020. godine o stavovima ispitanika o emigraciji i najnovija 2022. u okviru Projekta, također jasno potvrđuju nastavak depopulacije, prirodnog pada, nestanka učenika osnovnih i srednjih škola, pražnjenja hrvatskih ruralnih prostora, ugrožavanja osnovnih sustava i razvojne neizvjesnosti. Demografski se i migracijski nesklad treba početi ublažavati kao strateško nacionalno pitanje prema obama revitalizacijskima poticajnim modelima: klasičnom populacijskom politikom i selektivnom imigracijskom u interesu hrvatske razvojne budućnosti.
New feature for BioStor: extracting literature cited from OCR text
Authors: Page Roderic
Published: July 2023

At present BioStor provides a simple display of an article extracted from BHL. You get the page images, and sometimes a map and an altmetric "donut". But we can do better than this. For example, I'm starting to experiment with displaying a list of literature cited by the article.
Plan S: is there another way?
Authors: Moore Samuel
Published: July 2023

Over time, people (often administrators or regulatory agencies) try to control the tacking back-and forth, and especially, to standardize and make equivalent the ill-structured and well-structured aspects of the particular boundary object.Susan Leigh Star, ‘This Is Not a Boundary Object: Reflections on the Origin of a Concept’, p. 614.
Briefing Paper: Open Access Monographs
Authors: Moore Samuel
Published: July 2023

I recently wrote a briefing paper for Open Knowledge and PASTEUR4OA on open-access monographs. You can read it here.
CFF for Machine-Actionable Software Citations
Authors: Winston Donny
Published: July 2023

Add a CITATION.cff file to your git repository. The Citation File Format is automatically rendered on GitHub and usable by Zenodo and Zotero. Already have a DOI? Let’s see about a DOI-to-CFF tool. Looks like there’s doi2cff, but it’s currently restricted to DOIs on Zenodo that are tagged as software releases.
Indexing Identifiers
Authors: Winston Donny
Published: July 2023

Indexing identifiers is key to disambiguating entities. Wikipedia has disambiguation pages. For example, there are various concepts in mathematics and computing, various computing products, and various companies that identify with the term “Precision”. I made disambiguation pages for same-chemical-formula inorganic crystal structures for the Materials Project. Indexing identifiers is also key to unifying entities.
Indexing Identifier Services
Authors: Winston Donny
Published: July 2023

Where do you look for identifiers? If you’re looking for a URI, the IANA has a registry of schemes, like https, mailto, and tel. These days, to resolve an identifier, you generally use the https scheme, which has an authority component in its URI format.
Google Books and labour erasure in the digital humanities
Authors: Moore Samuel
Published: July 2023

I’m currently prepping to teach an article by Jean-Baptiste Michel and colleagues (paywalled) that presents an early (if not the first) analysis of the content digitised as part of the Google Books project. I ended up going down a rabbit hole trying to understand how the 25+ million books were actually scanned, as it wasn’t immediately clear.
New article – A genealogy of open access: negotiations between openness and access to research
Authors: Moore Samuel
Published: July 2023

I have a new article published in Revue française des sciences de l’information et de la communication (French Information and Communication Sciences Review). The piece is entitled ‘A genealogy of open access: negotiations between openness and access to research’ and looks at the various histories and lineages of OA to argue that it is best understood as a boundary object rather than a concept with a fixed definition or
DNA barcoding taxonomy now in GBIF
Authors: Page Roderic
Published: July 2023

Following on from adding DNA barcodes to GBIF I've now uploaded a taxonomic classification of DNA barcode BINs (Barcode Index Numbers). Each BIN is a cluster of similar DNA barcodes that is essentially equivalent to a species.
Library Band EP
Authors: Moore Samuel
Published: July 2023

In 2008 the Library Band recorded five songs in this cottage: Copyright Google, 2014. The Library Band was a four-piece ensemble whose members were either Book Movers or Book Fetchers at Cambridge University Library (yes, those are actual job titles). We played instrumental folky music composed of flute, accordion, oboe, xaphoon, and acoustic guitar.
Radical Open Access Collective
Authors: Moore Samuel
Published: July 2023

The new and updated website for the Radical Open Access Collective website is now live! https://radicaloa.co.uk Formed in 2015, the Radical OA Collective is a community of scholar-led, not-for-profit presses, journals and other open access projects in the humanities and social sciences. We represent an alternative open access ecosystem and seek to create a different future for open access, one based on experimenting with not-for-profit,
Compiling a C++ application to run on Heroku
Authors: Page Roderic
Published: July 2023

 TL;DR  Use a buildpack and set "LDFLAGS=--static" --disable-shared I use Heroku to host most of my websites, and since I mostly use PHP for web development this has worked fine. However, every so often I write an app that calls an external program written in, say, C++. Up until now I've had to host these apps on my own web servers. Today I finally bit the bullet and learned how to add a C++ program to a Heroku-hosted site.
A Perlisism for Identifiers: Delay Binding
Authors: Winston Donny
Published: July 2023

Inference based on semantic retrieval is more robust than inference based on syntactic parsing. In order to be authoritative, identifiers should be assigned as early as practicable in the creation process, but minting is not binding. Identifier resolution delays binding; identifier structures induce binding. Moral: Structure identifiers late (or never) in the minting process.
Validating an Identifier Service
Authors: Winston Donny
Published: July 2023

How do you validate that an identifier service provides global uniqueness of minted keys, persistence of bindings, and resolution of keys to descriptive metadata? If you know that a given ID provided by a service is unique, that tells you  nothing at all  about the uniqueness of another ID provided by that service.
Validating Traces — Syntactically, Semantically, and Situationally
Authors: Winston Donny
Published: July 2023

How do you validate a reified trace of digital-object provenance? Is it even possible? This is syntactic validation. Values that should be strings are strings, dates are dates, lists are lists, you know the drill… Is it plausible? This is semantic validation.
When Do Developers Not Have to Talk to Stakeholders?
Authors: Winston Donny
Published: July 2023

An ontologist can bridge  1  domain expertise and software development via production of a semi-informal so-called  intermediate representation   2  that can be understood by domain experts, and a formal ontology / knowledge graph that represents the domain in a machine-actionable way.
Significance of rescue hybrid endoscopic submucosal dissection in difficult colorectal cases
Authors: Yamaguchi Hayato , Fukuzawa Masakatsu , Kawai Takashi , Muramatsu Takahiro , Matsumoto Taisuke , Uchida Kumiko , Koyama Yohei , Madarame Akira , Morise Takashi , Kono Shin , Naito Sakiko , Nagata Naoyoshi , Sugimoto Mitsushige , Itoi Takao
Published: July 2023

Background/Aims: Hybrid endoscopic submucosal dissection (ESD), in which an incision is made around a lesion and snaring is performed after submucosal dissection, has some advantages in colorectal surgery, including shorter procedure time and preventing perforation. However, its value for rescue resection in difficult colorectal ESD cases remains unclear. This study evaluated the utility of rescue hybrid ESD (RH-ESD).Methods: We divided 364 colorectal ESD procedures into the conventional ESD group (C-ESD, n=260), scheduled hybrid ESD group (SH-ESD, n=69), and RH-ESD group (n=35) and compared their clinical outcomes.Results: Resection time was significantly shorter in the following order: RH-ESD (149 [90–197] minutes) >C-ESD (90 [60–140] minutes) >SH-ESD (52 [29–80] minutes). The en bloc resection rate increased significantly in the following order: RH-ESD (48.6%), SH-ESD (78.3%), and C-ESD (97.7%). An analysis of factors related to piecemeal resection of RH-ESD revealed that the submucosal dissection rate was significantly lower in the piecemeal resection group (25% [20%–30%]) than in the en bloc resection group (40% [20%–60%]).Conclusions: RH-ESD was ineffective in terms of curative resection because of the low en bloc resection rate, but was useful for avoiding surgery.
National identity development among recent immigrants: The role of perceived incompatibility
Authors: Suchowitz Isabelle , Fleischmann Fenella
Published: July 2023

This study longitudinally investigates the development of host-national identification among recently arrived immigrants and how it relates to origin-national and religious identification. We examine how implicit and explicit measures of identity incompatibility are related by including a measure of perceived value incompatibility into cross-lagged panel models of identification. We exploit three waves of panel data from the New Immigrant Survey Netherlands, targeting recent arrivals from Bulgaria (N = 151), Poland (N = 358), Spain (N = 298), and Turkey (N = 221). We found immigrants’ host-national identification to be relatively stable over time, whereas origin-national and religious identification underwent more changes, in group-specific ways. This suggests immigrants’ strategies to (re-)define their origin and religious identification may differ from strategies driving identification with their host country. Immigrants who perceive their identities to be incompatible do not necessarily reject the host-national identity, but might turn to the higher-status group to sustain a positive and distinct social identity.
Early breeding in the red fox in Great Britain
Authors: Smith Graham C , Cropper Paul , Halliwell Rebecca , Kent Alexander J
Published: July 2023

The red fox normally gives birth between February and April in Great Britain. Here we report two vixens from southern Wales that were pregnant or lactating in mid-December 2022, this matches the earliest record of breeding that we can find.
Quality of Life in Adult Cancer Patients With Underage Children: An Integrative Review
Authors: Mota-George, MSN, APRN, FNP-BC, AOCNP Gabriela , Martinez, DNP, APRN, FNP-BC, AOCNP, CBCN, CPHQ, NEA-BC Ashley , E. Dains, DrPH, JD, APRN, FNP-BC, FNAP, FAANP Joyce
Published: July 2023

As many as 24.7% of cancer patients are also parents to children younger than 18 years of age. This population faces unique challenges, and quality of life in parental cancer patients has not been well studied. This integrative review assessed parental cancer patients’ quality of life. PubMed and Scopus were searched using the following terms: quality of life, distress, anxiety, coping, emotion, social support, employment, work, psychosocial, physical, function, parental cancer, and parents with cancer. English publications conducted within the past 15 years that used an objective instrument to measure quality of life in adult cancer patients with children 18 years of age or younger were included. Studies with an intervention focus were excluded. After review of 672 articles, nine studies met the criteria for inclusion. Several instruments were utilized to measure quality of life. Some parental cancer patients reported decreased quality of life when compared with other cancer patients and the general population at diagnosis and years after. Parental cancer patients may be at an increased risk of decreased quality of life. With this understanding, health-care providers should complete comprehensive assessments routinely so that these patients’ unique needs may be more adequately addressed.
Can You Establish the Cause of This Patient’s Shortness of Breath?
Authors: Trail, PA-C Allison , Rogers, PharmD, BCOP Jane , Ajani, MD Jaffer
Published: July 2023

Mr. B is a 56-year-old man diagnosed with metastatic HER2-positive gastroesophageal adenocarcinoma. He received front-line leucovorin, 5-fluorouracil, and oxaliplatin (FOLFOX) and trastuzumab for 10 months before restaging imaging revealed progressive disease. He then received second-line trastuzumab deruxtecan. His treatment was complicated by several admissions felt to be unrelated to his cancer therapy. He was discharged after an episode of pneumonia on a steroid taper with prophylactic trimethoprim/sulfamethoxazole. Once he recovered, he was given a fourth dose of chemotherapy. About a week later, wheezes were noticed on physical exam, and he was given a 5-day course of levofloxacin. Around the same time, he also finished his steroid taper. Twelve days after his dose of chemotherapy, he presented to the emergency room with 3 to 4 days of progressive shortness of breath and dry cough following the completion of levofloxacin without symptom improvement. A CT scan showed increasing airspace opacities and multifocal areas of consolidation. Blood, nasal, and sputum cultures were negative. A bronchoscopy was performed that did not reveal findings concerning for capillaritis. He was ultimately diagnosed with drug-induced pneumonitis/interstitial lung disease (ILD). Mr. B continued to experience worsening hypoxic respiratory failure despite continuous IV steroids. He was discharged to an inpatient hospice facility where he passed away 2 weeks later. Drug-induced pneumonitis/ILD should be considered in all patients receiving trastuzumab deruxtecan who develop progressive shortness of breath or other respiratory complaints.
Đánh giá tác dụng điều trị tăng huyết áp độ I, I của viên nén thanh can HV trên lâm sàng
Authors: Lê Thị Thủy Tiên , Phạm Thủy Phương , Hoàng Trọng Quân , Phạm Quốc Bình
Published: July 2023

Mục tiêu: Đánh giá hiệu quả điều trị tăng huyết áp độ I, II của viên nén “Thanh Can HV” trên lâm sàng. Đối tượng nghiên cứu: Bệnh nhân từ 18 tuổi trở lên không phân biệt giới tính, nghề nghiệp, có chẩn đoán là tăng huyết áp nguyên phát độ 1, 2 (theo tiêu chuẩn khuyến cáo 2018 của Hội Tim mạch học Việt Nam). 

Phương pháp: Nghiên cứu can thiệp lâm sàng mở, so sánh trước và sau điều trị có nhóm đối chứng. 

Kết quả: Sử dụng viên nén Thanh Can HV điều trị bệnh nhân tăng huyết áp trong 28 ngày làm giảm có ý nghĩa thống kê các chỉ số huyết áp tâm thu, huyết áp tâm trương, huyết áp trung bình; 100% bệnh nhân sau sử dụng thuốc đều hạ huyết áp trong đó tỷ lệ đạt HA mục tiêu là 73,2%; Hiệu quả hạ áp tương đương với nhóm đối chứng sử dụng Amlodipine. Viên nén Thanh can HV có tác dụng cải thiện các triệu chứng lâm sàng tốt hơn thuốc Amlodipine, hiệu quả viên nén Thanh can HV đạt 92,7%.
Multi-response optimization of an extraction procedure of rosmarinic acid from Prunella vulgaris L. and antioxidants using
Authors: Do Thi Mai , Pham Thuy Phuong , Tran Thi Thu Hien , Pham Quoc Binh
Published: July 2023

Prunella vulgaris L. (PV) have been reported to have a variety of important biological activities. A process for rosmarinic acid (RA) extraction - antioxidants from PV was performed to obtain the highest extraction yield, strongest antioxidant activity and optimized by a multi-response optimization process. A three-level three-factor Box- Behnken design (BBD) was performed as response surface methodology (RSM) with desirability function (D) to attain the optimal extraction parameters. The rosmarinic acid extraction rate was determined by UV-Vis method with linear line Y = 2.1033x + 0.1108, R2 = 0.9966. The DPPH

and ABTS●+ scavenging percentage was used to represent the antioxidant ability. The maximum D value of 0.251, along with the maximum yield (6.025%), %RA (0.115%), scavenging percentage DPPH (IC50) (84.975 µg/ml) and ABTS

(TEAC) (0.207) were achieved after an ethanol

concentration of 60o, an extraction time of 60 min, using an extraction temperature of 50°C.
Tác dụng giảm đau và cải thiện tầm vận động cột sống cổ của phương pháp tác động cột sống kết hợp siêu âm trị liệu trên bệnh nhân đau vùng cổ gáy
Authors: Trần Thị Huệ , Nguyễn Tiến Chung
Published: July 2023

Mục tiêu: Đánh giá tác dụng giảm đau và cải thiện tầm vận động cột sống cổ của phương pháp tác động cột sống kết hợp siêu âm trị liệu ở bệnh nhân đau vùng cổ gáy do thoái hóa cột sống.
Đối tượng và Phương pháp: Nghiên cứu tiến cứu, can thiệp lâm sàng có đối chứng trên 70 bệnh nhân thoái hóa cột sống cổ có đau vùng cổ gáy, chia làm 2 nhóm bao gồm 35 bệnh nhân nhóm nghiên cứu (NNC) được điều trị bằng tác động cột sống kết hợp siêu âm trị liệu và 35 bệnh nhân nhóm đối chứng (NĐC) được điều trị bằng xoa bóp bấm huyệt kết hợp siêu âm trị liệu.
Kết quả: Mức độ đau, tầm vận động cột sống cổ (TVĐ) của cả 70 bệnh nhân được cải thiện theo thời gian; điểm đau VAS, điểm hạn chế tầm vận động cột sống cổ ở thời điểm sau điều trị thấp hơn có ý nghĩa thống kê so với thời điểm trước điều trị (p<0,05) và có xu hướng tốt hơn so với nhóm đối chứng.
Kết luận: Phương pháp tác động cột sống kết hợp siêu âm trị liệu có tác dụng giảm đau, cải thiện tầm vận động cột sống cổ trong điều trị đau vùng cổ gáy do thoái hóa cột sống cổ.
การพัฒนาวัสดุอ้างอิงรับรองสำหรับการวัดปริมาณธาตุบางชนิดในผงฟ้าทะลายโจร
Authors: ลี้เกรียงไกร อุษณา , ลายทิพย์ นันท์นภัส , แต่บรรพกุล สุทธินันท์ , ตั้งไพศาลกุล นงลักษณ์
Published: July 2023

สถาบันมาตรวิทยาแห่งชาติ (มว.) ได้พัฒนาวัสดุอ้างอิงรับรอง (Certified Reference Material, CRM) หรือชื่อทางการค้า คือ Thailand Reference Material (TRM) สำหรับการวัดปริมาณธาตุบางชนิดในผงฟ้าทะลายโจรขึ้น เพื่อช่วยสนับสนุนการวิเคราะห์วัตถุดิบ และผลิตภัณฑ์สมุนไพรให้มีความถูกต้อง และมีความน่าเชื่อถือโดยสามารถสอบย้อนกลับได้ทางมาตรวิทยา ซึ่ง มว. ได้รับการยอมรับในระดับสากลจากการประกาศความสามารถทางด้านการวัด Calibration and Measurement Capability (CMC) บนเว็บไซต์ของ International Bureau of Weight and Measures (BIPM) จากการเข้าร่วมเปรียบเทียบผลการวัดระหว่างประเทศในรายการต่าง ๆ เช่น CCQM-K89 เป็นต้น ทำให้ผลการวัดโลหะหนักในสมุนไพรมีความน่าเชื่อถือในระดับนานาชาติ ดังนั้น มว. จึงอาศัยความสามารถดังกล่าวให้ค่าอ้างอิง (Certified value) และค่าความไม่แน่นอนของการวัด (Measurement uncertainty) ใน TRM-F-2006 สำหรับธาตุ As, Ca, Cd, Cr, Cu, Fe, Mg, Mn, Ni, P, Pb และ Zn  รวมถึงให้ค่าเพื่อเป็นข้อมูล (Information value) สำหรับธาตุ Hg และ Na ด้วยเทคนิคต่าง ๆ ได้แก่ ID-ICP-MS, GSA-ICP-MS, GSA-ICP-OES, Ext-ICP-MS และ Ext-ICP-OES โดย TRM-F-2006 ที่พัฒนาขึ้นนี้มีกระบวนการผลิตเป็นไปตามมาตรฐานการผลิตวัสดุอ้างอิงรับรองตามระบบคุณภาพ ISO 17034 ซึ่งพบว่าตัวอย่างผงฟ้าทะลายโจรมีความเป็นเนื้อเดียวกัน และมีความเสถียรทั้งระยะสั้น และระยะยาว ดังนั้นจึงสามารถนำ TRM-F-2006 ไปใช้ในการตรวจสอบความถูกต้องของวิธีทดสอบ รวมถึงนำไปใช้ในการควบคุมคุณภาพของห้องปฏิบัติการได้
ORGANISASI SEBAGAI ARENA KEKUASAAN POLITIK
Authors: Kardiat Yosar
Published: July 2023

This article discusses the organization as a vehicle (arena) in political power. Organization andpolitics become one unit in the use of power in Indonesia. In essence, the use of power in politicsaims to regulate the interests of the community as a whole, not for personal or group interests.For this reason, the limitation of power is very necessary in order to grow public trust in those inpower and create justice and comfort in life. Seeing organizational activities as political activitiesis a refresher on the understanding of organizational life which has always been dominated by aninstrumental perspective, whose analysis ignores the motives and interests of the actors involvedin the organization.
Child Health Detection System Along with the Recommendations Using K-Nearest Neighbor Artificial Intelligence
Authors: Khairulisni saniati  , Kridiana Wijayanti  , Kurnianingsih
Published: July 2023

Background: Children under 5 years of age were very vulnerable to disease. Therefore, child health is one of the main focuses in the health sector. The main triggers for under-five mortality were preventable and treatable diseases. There were 5 diseases mostly experienced by under-five children, namely pneumonia, diarrhea, malaria, measles and malnutrition. In Indonesia, child health detection is still performed manually by midwives using the IMCI cards, but during the Covid-19 pandemic, child health detection is not actively performed. The K-Nearest Neighbor Artificial Intelligence System can be used for child health detection.

Objective: To develop a child health detection system along with the recommendations using k-nearest neighbor artificial intelligence.

Methods: Research and Development (RnD) and system accuracy test. The study subjects involved secondary data of 1000 IMCI register data. Data were tested using the intra-class correlation coefficient and the K-Nearest Neighbor algorithm.

Results: The results of the child health detection system using k-nearest neighbor artificial intelligence obtained a system accuracy rate of 100% and the sick child health recommendation system using k-nearest neighbor artificial intelligence obtained a system accuracy rate of 89.3%. It was proven that k-nearest neighbor artificial intelligence was able to develop a child health detection system along with the recommendations which can be used by parents.
Numerical simulation of erosion of misaligned W/Cu monoblocks caused by ITER-like type-Iedge localized modes
Authors: Huang Yan , Sun Ji-Zhong , Sang Chao-Feng , Wang De-Zhen ,   Department of Basic Courses Teaching, Dalian Polytechnic University, Dalian 116034, China,   School of Physics, Dalian University of Technology, Dalian 116024, China
Published: July 2023

The thermal performance of the divertor W/Cu monoblock tiles under the ITER-like transient events has been one of the main concerns for ITER plasma facing components. Owing to the assembly tolerances during installation, the leading edge caused by misalignment between toroidal neighboring tiles will receive the extremely high cumulative heat flux and be damaged. In this work, we develop a two-dimensional heat conductivity model, including evaporation, radiation, melting process, and coupling cooling water condition, to investigate the thermal erosion of two shapes of tiles of W/Cu monoblock (unshaped and beveled tiles) with misalignment in a range from 0 to 0.3 mm, within the allowable maximum misalignment for ITER. To reflect the geometrical effects of castellated divertor tiles on the properties of its adjacent plasma, the energy flux density distribution arriving at the castellated divertor tile surface is evaluated first by using a two-dimension-in-space and three-dimension-in-velocity particle-in-cell plus Monte Carlo collisions code, and the obtained energy flux distribution is then used as input for the heat conduction model. The simulation results show that the maximum temperature of the unshaped tile with no misalignment is lower than that of the beveled tile under the steady-state inter-ELM heat flux, which increases more quickly than that of the beveled tile with misalignment increasing and will be larger than that of the beveled tile when misalignment is not less than 0.105 mm. Two shapes of the divertor tiles would melt and vaporize under typical heat flux density of a transient event of type-I edge localized modes (ELMs) for ITER, deposition energy of 1 MJ·m–2 in a duration of 600 μs. The highest temperature, the maximum melting thickness, the maximum vaporization thickness of the unshaped tile with no misalignment are higher than those of the beveled tile except the melting volume ratio. The thermal erosion of the unshaped tile increases more remarkably than that of the beveled tile with misalignment increasing, and the melting volume ratio of the unshaped tile will exceed that of the beveled tile at a misalignment of 0.17 mm. In comparison with the unshaped tile, the beveled tile is more resistant to such a high heat flux of an ELM and misalignment.
Short-range interactions between fibrocytes and CD8+ T cells in COPD bronchial inflammatory response
Authors: Eyraud Edmée , Maurat Elise , Sac-Epée Jean-Marc , Henrot Pauline , Zysman Maeva , Esteves Pauline , Trian Thomas , Dupuy Jean-William , Leipold Alexander , Saliba Antoine-Emmanuel , Begueret Hugues , Girodet Pierre-Olivier , Thumerel Matthieu , Hustache-Castaing Romain , Marthan Roger , Levet Florian , Vallois Pierre , Contin-Bordes Cécile , Berger Patrick , Dupin Isabelle
Published: July 2023

Bronchi of chronic obstructive pulmonary disease (COPD) are the site of extensive cell infiltration, allowing persistent contact between resident cells and immune cells. Tissue fibrocytes interaction with CD8+ T cells and its consequences were investigated using a combination of in situ, in vitro experiments and mathematical modeling. We show that fibrocytes and CD8+ T cells are found in the vicinity of distal airways and that potential interactions are more frequent in tissues from COPD patients compared to those of control subjects. Increased proximity and clusterization between CD8+ T cells and fibrocytes are associated with altered lung function. Tissular CD8+ T cells from COPD patients promote fibrocyte chemotaxis via the CXCL8-CXCR1/2 axis. Live imaging shows that CD8+ T cells establish short-term interactions with fibrocytes, that trigger CD8+ T cell proliferation in a CD54- and CD86-dependent manner, pro-inflammatory cytokines production, CD8+ T cell cytotoxic activity against bronchial epithelial cells and fibrocyte immunomodulatory properties. We defined a computational model describing these intercellular interactions and calibrated the parameters based on our experimental measurements. We show the model’s ability to reproduce histological ex vivo characteristics, and observe an important contribution of fibrocyte-mediated CD8+ T cell proliferation in COPD development. Using the model to test therapeutic scenarios, we predict a recovery time of several years, and the failure of targeting chemotaxis or interacting processes. Altogether, our study reveals that local interactions between fibrocytes and CD8+ T cells could jeopardize the balance between protective immunity and chronic inflammation in the bronchi of COPD patients.
Leveraging genetic diversity to identify small molecules that reverse mouse skeletal muscle insulin resistance
Authors: Masson Stewart WC , Madsen Søren , Cooke Kristen C , Potter Meg , Vegas Alexis Diaz , Carroll Luke , Thillainadesan Senthil , Cutler Harry B , Walder Ken R , Cooney Gregory J , Morahan Grant , Stöckli Jacqueline , James David E
Published: July 2023

Systems genetics has begun to tackle the complexity of insulin resistance by capitalising on computational advances to study high-diversity populations. ‘Diversity Outbred in Australia (DOz)’ is a population of genetically unique mice with profound metabolic heterogeneity. We leveraged this variance to explore skeletal muscle’s contribution to whole-body insulin action through metabolic phenotyping and skeletal muscle proteomics of 215 DOz mice. Linear modelling identified 553 proteins that associated with whole-body insulin sensitivity (Matsuda Index) including regulators of endocytosis and muscle proteostasis. To enrich for causality, we refined this network by focusing on negatively associated, genetically regulated proteins, resulting in a 76-protein fingerprint of insulin resistance. We sought to perturb this network and restore insulin action with small molecules by integrating the Broad Institute Connectivity Map platform and in vitro assays of insulin action using the Prestwick chemical library. These complementary approaches identified the antibiotic thiostrepton as an insulin resistance reversal agent. Subsequent validation in ex vivo insulin-resistant mouse muscle and palmitate-induced insulin-resistant myotubes demonstrated potent insulin action restoration, potentially via upregulation of glycolysis. This work demonstrates the value of a drug-centric framework to validate systems-level analysis by identifying potential therapeutics for insulin resistance.
Tools and methods for high-throughput single-cell imaging with the mother machine
Authors: Thiermann Ryan , Sandler Michael , Ahir Gursharan , Sauls John T , Schroeder Jeremy , Brown Steven , Le Treut Guillaume , Si Fangwei , Li Dongyang , Wang Jue D , Jun Suckjoon
Published: July 2023

Despite much progress, image processing remains a significant bottleneck for high-throughput analysis of microscopy data. One popular platform for single-cell time-lapse imaging is the mother machine, which enables long-term tracking of microbial cells under precisely controlled growth conditions. While several mother machine image analysis pipelines have been developed in the past several years, adoption by a non-expert audience remains a challenge. To fill this gap, we implemented our own software, MM3, as a plugin for the multidimensional image viewer napari. napari-MM3 is a complete and modular image analysis pipeline for mother machine data, which takes advantage of the high-level interactivity of napari. Here, we give an overview of napari-MM3 and test it against several well-designed and widely used image analysis pipelines, including BACMMAN and DeLTA. Researchers often analyze mother machine data with custom scripts using varied image analysis methods, but a quantitative comparison of the output of different pipelines has been lacking. To this end, we show that key single-cell physiological parameter correlations and distributions are robust to the choice of analysis method. However, we also find that small changes in thresholding parameters can systematically alter parameters extracted from single-cell imaging experiments. Moreover, we explicitly show that in deep learning-based segmentation, ‘what you put is what you get’ (WYPIWYG) – that is, pixel-level variation in training data for cell segmentation can propagate to the model output and bias spatial and temporal measurements. Finally, while the primary purpose of this work is to introduce the image analysis software that we have developed over the last decade in our lab, we also provide information for those who want to implement mother machine-based high-throughput imaging and analysis methods in their research.
The Pseudokinase Ulk4 promotes Shh signaling by regulating Stk36 ciliary localization and Gli2 phosphorylation
Authors: Zhou Mengmeng , Han Yuhong , Jiang Jin
Published: July 2023

The Hedgehog (Hh) family of secreted proteins governs embryonic development and adult tissue homeostasis through the Gli family of transcription factors. Gli is thought to be activated at the tip of primary cilium, but the underlying mechanism remains poorly understood. Here, we show that
U
nc-51-like
k
inase 4 (Ulk4), a pseudokinase and a member of the Ulk kinase family, acts in conjunction with another Ulk family member Stk36 to promote Gli2 phosphorylation and Hh pathway activation. Ulk4 interacts with Stk36 through its N-terminal region containing the pseudokinase domain and Gli2 via its regulatory domain to bridge the kinase and substrate. Although dispensable for Hh-induced Stk36 kinase activation, Ulk4 is essential for Stk36 ciliary tip localization, Gli2 phosphorylation and activation. In response to Hh, both Ulk4 and Stk36 colocalize with Gli2 at ciliary tip, and Ulk4 and Stk36 depend on each other for their ciliary tip accumulation. We further show that ciliary localization of Ulk4 depends on Stk36 kinase activity and phosphorylation of Ulk4 on Thr1023, and that ciliary tip accumulation of Ulk4 is essential for its function in the Hh pathway. Taken together, our results suggest that Ulk4 regulates Hh signaling by promoting Stk36-mediated Gli2 phosphorylation and activation at ciliary tip.
Reprogramming of cardiac phosphoproteome, proteome, and transcriptome confers resilience to chronic adenylyl cyclase-driven stress
Authors: Qu Jia-Hua , Chakir Khalid , Tarasov Kirill V , Riordon Daniel R , Perino Maria Grazia , Silvester Allwin Jennifa , Lakatta Edward G
Published: July 2023

Our prior study (Tarasov et al., 2022) discovered that numerous adaptive mechanisms emerge in response to cardiac-specific overexpression of adenylyl cyclase type 8 (TGAC8) which included overexpression of a large number of proteins. Here, we conducted an unbiased phosphoproteomics analysis in order to determine the role of altered protein phosphorylation in the adaptive heart performance and protection profile of adult TGAC8 left ventricle (LV) at 3–4 months of age, and integrated the phosphoproteome with transcriptome and proteome. Based on differentially regulated phosphoproteins by genotype, numerous stress-response pathways within reprogrammed TGAC8 LV, including PKA, PI3K, and AMPK signaling pathways, predicted upstream regulators (e.g. PDPK1, PAK1, and PTK2B), and downstream functions (e.g. cell viability, protein quality control), and metabolism were enriched. In addition to PKA, numerous other kinases and phosphatases were hyper-phosphorylated in TGAC8 vs. WT. Hyper-phosphorylated transcriptional factors in TGAC8 were associated with increased mRNA transcription, immune responses, and metabolic pathways. Combination of the phosphoproteome with its proteome and with the previously published TGAC8 transcriptome enabled the elucidation of cardiac performance and adaptive protection profiles coordinately regulated at post-translational modification (PTM) (phosphorylation), translational, and transcriptional levels. Many stress-response signaling pathways, i.e., PI3K/AKT, ERK/MAPK, and ubiquitin labeling, were consistently enriched and activated in the TGAC8 LV at transcriptional, translational, and PTM levels. Thus, reprogramming of the cardiac phosphoproteome, proteome, and transcriptome confers resilience to chronic adenylyl cyclase-driven stress. We identified numerous pathways/function predictions via gene sets, phosphopeptides, and phosphoproteins, which may point to potential novel therapeutic targets to enhance heart adaptivity, maintaining heart performance while avoiding cardiac dysfunction.
Quorum-sensing agr system of Staphylococcus aureus primes gene expression for protection from lethal oxidative stress
Authors: Podkowik Magdalena , Perault Andrew I. , Putzel Gregory , Pountain Andrew , Kim Jisun , Dumont Ashley , Zwack Erin , Ulrich Robert J. , Karagounis Theodora K. , Zhou Chunyi , Haag Andreas F. , Shenderovich Julia , Wasserman Gregory A. , Kwon Junbeom , Chen John , Richardson Anthony R. , Weiser Jeffrey N. , Nowosad Carla R. , Lun Desmond S. , Parker Dane , Pironti Alejandro , Zhao Xilin , Drlica Karl , Yanai Itai , Torres Victor J. , Shopsin Bo
Published: July 2023

The
agr
quorum-sensing system links
Staphylococcus aureus
metabolism to virulence, in part by increasing bacterial survival during exposure to lethal concentrations of H
2
O
2
, a crucial host defense against
S. aureus
. We now report that protection by
agr
surprisingly extends beyond post-exponential growth to the exit from stationary phase when the
agr
system is no longer turned on. Thus,
agr
can be considered a constitutive protective factor. Deletion of
agr
increased both respiration and aerobic fermentation but decreased ATP levels and growth, suggesting that Δ
agr
cells assume a hyperactive metabolic state in response to reduced metabolic efficiency. As expected from increased respiratory gene expression, reactive oxygen species (ROS) accumulated more in the
agr
mutant than in wild-type cells, thereby explaining elevated susceptibility of Δ
agr
strains to lethal H
2
O
2
doses. Increased survival of wild-type
agr
cells during H
2
O
2
exposure required
sodA
, which detoxifies superoxide. Additionally, pretreatment of
S. aureus
with respiration-reducing menadione protected Δ
agr
cells from killing by H
2
O
2
. Thus, genetic deletion and pharmacologic experiments indicate that
agr
helps control endogenous ROS, thereby providing resilience against exogenous ROS. The long-lived “memory” of
agr
-mediated protection, which is uncoupled from
agr
activation kinetics, increased hematogenous dissemination to certain tissues during sepsis in ROS-producing, wild-type mice but not ROS-deficient (Nox2
−/−
) mice. These results demonstrate the importance of protection that anticipates impending ROS-mediated immune attack. The ubiquity of quorum sensing suggests that it protects many bacterial species from oxidative damage.