bluuebunny/crossref_metadata_2025 huggingface dataset:
Songlines Are for Singing: Un/Mapping the Lived Spaces of Travelling Memory
Authors: Roberts Les
Published: June 2023

Putting to work the dialectical concept of ‘un/mapping’, this paper examines the immateriality of cultural memory as coalescent in and around songlines: spatial stories woven from the autobiogeographical braiding of music and memory. Borrowing from Erll’s concept of ‘travelling memory’ (2011), the idea of songlines provides a performative framework with which to both travel with music memory and to map/unmap the travelling of music memory. The theoretical focus of the work builds on empirical studies into music, place and cultural memory in the form of interviews conducted across the UK in 2010–2013. The interviews were designed to explore the way peoples’ musical pasts—memories of listening to music in the domestic home, for example, or attendance at concerts and festivals, music as soundtracks to journeys, holidays or everyday commutes to work or school, music at key rite of passage moments—have coloured and given shape to the narratives that structure a sense of embodied selfhood and social identity over time. Songlines, it is shown, tether the self to spaces and temporalities that map a tangled meshwork of lives lived spatially, where the ghosts of musical pasts are as vital and alive as the traveller who has invoked them. Analysis and discussion is centred around the following questions: How should the songlines of memory be mapped in ways that remain true and resonant with those whose spatial stories they tell? How, phenomenologically, can memory be rendered as an energy that remains creatively vital without running the risk of dissipating that energy by seeking to fix it in space and time (to memorialise it)? And if, as is advocated in the paper, we should not be in the business of mapping songlines, how do we go about the task of singing them? Pursuing these and other lines of enquiry, this paper explores a spatial anthropology of movement and travel in which the un/mapping of popular music memory mobilises phenomenological understandings of the entanglements of self, culture and embodied memory.
Inconsistency Detection in Cross-Layer Tile Maps with Super-Pixel Segmentation
Authors: Yu Junbo , Ai Tinghua , Xu Haijiang , Yan Lingrui , Shen Yilang
Published: June 2023

The consistency of geospatial data is of great significance for the application and updating of geographic information in web maps. Due to the multiple data sources and different temporal versions, the tile web maps usually meet the inconsistency question across different layers. This study tries to develop a method to detect this kind of inconsistency utilizing a raster-based scaling approach. Compared with vector-based handling, this method can be directly available for multi-level tile images in a pixel representation form. The proposed cross-layer raster tile map rendering method (CRTMRM) consists of four primary aspects: geographic object separation, consistency rendering rules, data scaling and derivation with super-pixel segmentation, and inconsistency detection. The scale transformation strategy with the super-pixel attempts to obtain a simplified representation. Taking the scale lifespan variation and geometric consistency rules into account, the inconsistency detection of tile maps is conducted between temporal versions, multi-sources, and different scales through actual and derived data overlay analysis. The experiment focuses on features of cross-layer water or vegetation areas with Level 9 to Level 14 in Baidu Maps, Amap, and Google Maps. This method is able to serve as a basis for massive unstructured web map data inconsistency detection and support intelligent web map rendering.
Single-Molecule Analysis of the Improved Variants of the G-Quadruplex Recognition Protein G4P
Authors: Gaur Paras , Bain Fletcher E. , Honda Masayoshi , Granger Sophie L. , Spies Maria
Published: June 2023

As many as 700,000 unique sequences in the human genome are predicted to fold into G-quadruplexes (G4s), non-canonical structures formed by Hoogsteen guanine–guanine pairing within G-rich nucleic acids. G4s play both physiological and pathological roles in many vital cellular processes including DNA replication, DNA repair and RNA transcription. Several reagents have been developed to visualize G4s in vitro and in cells. Recently, Zhen et al. synthesized a small protein G4P based on the G4 recognition motif from RHAU (DHX36) helicase (RHAU specific motif, RSM). G4P was reported to bind the G4 structures in cells and in vitro, and to display better selectivity toward G4s than the previously published BG4 antibody. To get insight into G4P- G4 interaction kinetics and selectivity, we purified G4P and its expanded variants, and analyzed their G4 binding using single-molecule total internal reflection fluorescence microscopy and mass photometry. We found that G4P binds to various G4s with affinities defined mostly by the association rate. Doubling the number of the RSM units in the G4P increases the protein’s affinity for telomeric G4s and its ability to interact with sequences folding into multiple G4s.
Effects of Fructose and Palmitic Acid on Gene Expression in Drosophila melanogaster Larvae: Implications for Neurodegenerative Diseases
Authors: Santos-Cruz Luis Felipe , Sigrist-Flores Santiago Cristobal , Castañeda-Partida Laura , Heres-Pulido María Eugenia , Dueñas-García Irma Elena , Piedra-Ibarra Elías , Ponciano-Gómez Alberto , Jiménez-Flores Rafael , Campos-Aguilar Myriam
Published: June 2023

One of the largest health problems worldwide is the development of chronic noncommunicable diseases due to the consumption of hypercaloric diets. Among the most common alterations are cardiovascular diseases, and a high correlation between overnutrition and neurodegenerative diseases has also been found. The urgency in the study of specific damage to tissues such as the brain and intestine led us to use Drosophila melanogaster to study the metabolic effects caused by the consumption of fructose and palmitic acid in specific tissues. Thus, third instar larvae (96 ± 4 h) of the wild Canton-S strain of D. melanogaster were used to perform transcriptomic profiling in brain and midgut tissues to test for the potential metabolic effects of a diet supplemented with fructose and palmitic acid. Our data infer that this diet can alter the biosynthesis of proteins at the mRNA level that participate in the synthesis of amino acids, as well as fundamental enzymes for the dopaminergic and GABAergic systems in the midgut and brain. These also demonstrated alterations in the tissues of flies that may help explain the development of various reported human diseases associated with the consumption of fructose and palmitic acid in humans. These studies will not only help to better understand the mechanisms by which the consumption of these alimentary products is related to the development of neuronal diseases but may also contribute to the prevention of these conditions.
Characterization and Biological Activities of Melanin from the Medicinal Fungi Ophiocordyceps sinensis
Authors: Tong Chaoqun , Luo Jian , Xie Chaolu , Wei Junhong , Pan Guoqing , Zhou Zeyang , Li Chunfeng
Published: June 2023

Melanin is a complex natural pigment that is widely present in fungi. The mushroom Ophiocordyceps sinensis has a variety of pharmacological effects. The active substances of O. sinensis have been extensively studied, but few studies have focused on the O. sinensis melanin. In this study, the production of melanin was increased by adding light or oxidative stress, namely, reactive oxygen species (ROS) or reactive nitrogen species (RNS), during liquid fermentation. Subsequently, the structure of the purified melanin was characterized using elemental analysis, ultraviolet-visible absorption spectrum, Fourier transform infrared (FTIR), electron paramagnetic resonance (EPR), and pyrolysis gas chromatography and mass spectrometry (Py-GCMS). Studies have shown that O. sinensis melanin is composed of C (50.59), H (6.18), O (33.90), N (8.19), and S (1.20), with maximum absorbance at 237 nm and typical melanin structures such as benzene, indole, and pyrrole. Additionally, the various biological activities of O. sinensis melanin have been discovered; it can chelate heavy metals and shows a strong ultraviolet-blocking ability. Moreover, O. sinensis melanin can reduce the levels of intracellular reactive oxygen species and counteract the oxidative damage of H2O2 to cells. These results can help us to develop applications of O. sinensis melanin in radiation resistance, heavy metal pollution remediation, and antioxidant use.
Extracellular Vesicles and Cx43-Gap Junction Channels Are the Main Routes for Mitochondrial Transfer from Ultra-Purified Mesenchymal Stem Cells, RECs
Authors: Yang Jiahao , Liu Lu , Oda Yasuaki , Wada Keisuke , Ago Mako , Matsuda Shinichiro , Hattori Miho , Goto Tsukimi , Ishibashi Shuichi , Kawashima-Sonoyama Yuki , Matsuzaki Yumi , Taketani Takeshi
Published: June 2023

Mitochondria are essential organelles for maintaining intracellular homeostasis. Their dysfunction can directly or indirectly affect cell functioning and is linked to multiple diseases. Donation of exogenous mitochondria is potentially a viable therapeutic strategy. For this, selecting appropriate donors of exogenous mitochondria is critical. We previously demonstrated that ultra-purified bone marrow-derived mesenchymal stem cells (RECs) have better stem cell properties and homogeneity than conventionally cultured bone marrow-derived mesenchymal stem cells. Here, we explored the effect of contact and noncontact systems on three possible mitochondrial transfer mechanisms involving tunneling nanotubes, connexin 43 (Cx43)-mediated gap junction channels (GJCs), and extracellular vesicles (Evs). We show that Evs and Cx43-GJCs provide the main mechanism for mitochondrial transfer from RECs. Through these two critical mitochondrial transfer pathways, RECs could transfer a greater number of mitochondria into mitochondria-deficient (ρ0) cells and could significantly restore mitochondrial functional parameters. Furthermore, we analyzed the effect of exosomes (EXO) on the rate of mitochondrial transfer from RECs and recovery of mitochondrial function. REC-derived EXO appeared to promote mitochondrial transfer and slightly improve the recovery of mtDNA content and oxidative phosphorylation in ρ0 cells. Thus, ultrapure, homogenous, and safe stem cell RECs could provide a potential therapeutic tool for diseases associated with mitochondrial dysfunction.
Effect of Chemical Chaperones on the Stability of Proteins during Heat– or Freeze–Thaw Stress
Authors: Borzova Vera A. , Eronina Tatiana B. , Mikhaylova Valeriya V. , Roman Svetlana G. , Chernikov Andrey M. , Chebotareva Natalia A.
Published: June 2023

The importance of studying the structural stability of proteins is determined by the structure–function relationship. Protein stability is influenced by many factors among which are freeze–thaw and thermal stresses. The effect of trehalose, betaine, sorbitol and 2-hydroxypropyl-β-cyclodextrin (HPCD) on the stability and aggregation of bovine liver glutamate dehydrogenase (GDH) upon heating at 50 °C or freeze–thawing was studied by dynamic light scattering, differential scanning calorimetry, analytical ultracentrifugation and circular dichroism spectroscopy. A freeze–thaw cycle resulted in the complete loss of the secondary and tertiary structure, and aggregation of GDH. All the cosolutes suppressed freeze–thaw- and heat-induced aggregation of GDH and increased the protein thermal stability. The effective concentrations of the cosolutes during freeze–thawing were lower than during heating. Sorbitol exhibited the highest anti-aggregation activity under freeze–thaw stress, whereas the most effective agents stabilizing the tertiary structure of GDH were HPCD and betaine. HPCD and trehalose were the most effective agents suppressing GDH thermal aggregation. All the chemical chaperones stabilized various soluble oligomeric forms of GDH against both types of stress. The data on GDH were compared with the effects of the same cosolutes on glycogen phosphorylase b during thermal and freeze–thaw-induced aggregation. This research can find further application in biotechnology and pharmaceutics.
Insight into Population Structure and Drug Resistance of Pediatric Tuberculosis Strains from China and Russia Gained through Whole-Genome Sequencing
Authors: Zhdanova Svetlana , Jiao Wei-Wei , Sinkov Viacheslav , Khromova Polina , Solovieva Natalia , Mushkin Alexander , Mokrousov Igor , Belopolskaya Olesya , Masharsky Aleksey , Vyazovaya Anna , Rychkova Lubov , Kolesnikova Lubov , Zhuravlev Viacheslav , Shen A-Dong , Ogarkov Oleg
Published: June 2023

This study aimed to determine phenotypic and genotypic drug resistance patterns of Mycobacterium tuberculosis strains from children with tuberculosis (TB) in China and Russia, two high-burden countries for multi/extensively-drug resistant (MDR/XDR) TB. Whole-genome sequencing data of M. tuberculosis isolates from China (n = 137) and Russia (n = 60) were analyzed for phylogenetic markers and drug-resistance mutations, followed by comparison with phenotypic susceptibility data. The Beijing genotype was detected in 126 Chinese and 50 Russian isolates. The Euro-American lineage was detected in 10 Russian and 11 Chinese isolates. In the Russian collection, the Beijing genotype and Beijing B0/W148-cluster were dominated by MDR strains (68% and 94%, respectively). Ninety percent of B0/W148 strains were phenotypically pre-XDR. In the Chinese collection, neither of the Beijing sublineages was associated with MDR/pre-XDR status. MDR was mostly caused by low fitness cost mutations (rpoB S450L, katG S315T, rpsL K43R). Chinese rifampicin-resistant strains demonstrated a higher diversity of resistance mutations than Russian isolates (p = 0.003). The rifampicin and isoniazid resistance compensatory mutations were detected in some MDR strains, but they were not widespread. The molecular mechanisms of M. tuberculosis adaptation to anti-TB treatment are not unique to the pediatric strains, but they reflect the general situation with TB in Russia and China.
CD109 Promotes Drug Resistance in A2780 Ovarian Cancer Cells by Regulating the STAT3-NOTCH1 Signaling Axis
Authors: Kim Jun Se , Shin Min Joo , Lee Seo Yul , Kim Dae Kyoung , Choi Kyung-Un , Suh Dong-Soo , Kim Dayea , Kim Jae Ho
Published: June 2023

Epithelial ovarian cancer (EOC) is the most lethal gynecological malignancy owing to relapse caused by resistance to chemotherapy. We previously reported that cluster of differentiation 109 (CD109) expression is positively correlated with poor prognosis and chemoresistance in patients with EOC. To further explore the role of CD109 in EOC, we explored the signaling mechanism of CD109-induced drug resistance. We found that CD109 expression was upregulated in doxorubicin-resistant EOC cells (A2780-R) compared with that in their parental cells. In EOC cells (A2780 and A2780-R), the expression level of CD109 was positively correlated with the expression level of ATP-binding cassette (ABC) transporters, such as ABCB1 and ABCG2, and paclitaxel (PTX) resistance. Using a xenograft mouse model, it was confirmed that PTX administration in xenografts of CD109-silenced A2780-R cells significantly attenuated in vivo tumor growth. The treatment of CD109-overexpressed A2780 cells with cryptotanshinone (CPT), a signal transducer and activator of transcription 3 (STAT3) inhibitor, inhibited the CD109 overexpression-induced activation of STAT3 and neurogenic locus notch homolog protein 1 (NOTCH1), suggesting a STAT3-NOTCH1 signaling axis. The combined treatment of CD109-overexpressed A2780 cells with CPT and N-[N-(3,5-difluorophenacetyl)-l-alanyl]-S-phenylglycine t-butyl ester (DAPT), a NOTCH inhibitor, markedly abrogated PTX resistance. These results suggest that CD109 plays a key role in the acquisition of drug resistance by activating the STAT3-NOTCH1 signaling axis in patients with EOC.
The Crosstalk between Gut Microbiota and Nervous System: A Bidirectional Interaction between Microorganisms and Metabolome
Authors: Montagnani Monica , Bottalico Lucrezia , Potenza Maria Assunta , Charitos Ioannis Alexandros , Topi Skender , Colella Marica , Santacroce Luigi
Published: June 2023

Several studies have shown that the gut microbiota influences behavior and, in turn, changes in the immune system associated with symptoms of depression or anxiety disorder may be mirrored by corresponding changes in the gut microbiota. Although the composition/function of the intestinal microbiota appears to affect the central nervous system (CNS) activities through multiple mechanisms, accurate epidemiological evidence that clearly explains the connection between the CNS pathology and the intestinal dysbiosis is not yet available. The enteric nervous system (ENS) is a separate branch of the autonomic nervous system (ANS) and the largest part of the peripheral nervous system (PNS). It is composed of a vast and complex network of neurons which communicate via several neuromodulators and neurotransmitters, like those found in the CNS. Interestingly, despite its tight connections to both the PNS and ANS, the ENS is also capable of some independent activities. This concept, together with the suggested role played by intestinal microorganisms and the metabolome in the onset and progression of CNS neurological (neurodegenerative, autoimmune) and psychopathological (depression, anxiety disorders, autism) diseases, explains the large number of investigations exploring the functional role and the physiopathological implications of the gut microbiota/brain axis.
Towards a Unified Architecture Powering Scalable Learning Models with IoT Data Streams, Blockchain, and Open Data
Authors: Debauche Olivier , Nkamla Penka Jean Bertin , Hani Moad , Guttadauria Adriano , Ait Abdelouahid Rachida , Gasmi Kaouther , Ben Hardouz Ouafae , Lebeau Frédéric , Bindelle Jérôme , Soyeurt Hélène , Gengler Nicolas , Manneback Pierre , Benjelloun Mohammed , Bertozzi Carlo
Published: June 2023

The huge amount of data produced by the Internet of Things need to be validated and curated to be prepared for the selection of relevant data in order to prototype models, train them, and serve the model. On the other side, blockchains and open data are also important data sources that need to be integrated into the proposed integrative models. It is difficult to find a sufficiently versatile and agnostic architecture based on the main machine learning frameworks that facilitate model development and allow continuous training to continuously improve them from the data streams. The paper describes the conceptualization, implementation, and testing of a new architecture that proposes a use case agnostic processing chain. The proposed architecture is mainly built around the Apache Submarine, an unified Machine Learning platform that facilitates the training and deployment of algorithms. Here, Internet of Things data are collected and formatted at the edge level. They are then processed and validated at the fog level. On the other hand, open data and blockchain data via Blockchain Access Layer are directly processed at the cloud level. Finally, the data are preprocessed to feed scalable machine learning algorithms.
Navigating Privacy and Data Safety: The Implications of Increased Online Activity among Older Adults Post-COVID-19 Induced Isolation
Authors: Alagood John , Prybutok Gayle , Prybutok Victor R.
Published: June 2023

The COVID-19 pandemic spurred older adults to use information and communication technology (ICT) for maintaining connections and engagement during social distancing. This trend raises concerns about privacy and data safety for older individuals with limited technical knowledge who have adopted ICT reluctantly and may be distinct in their susceptibility to scams, fraud, and identity theft. This paper highlights the gap in the literature regarding the increased privacy and data security risks for older adults adopting technology due to isolation during the pandemic (referred to here as quarantine technology initiates (QTIs)). A literature search informed by healthcare experts explored the intersection of older adults, data privacy, online activity, and COVID-19. A thin and geographically diverse literature was found to consider the risk profile of QTIs with the same lens as for older adults who adopted ICT before or independent of COVID-19 quarantines. The mentioned strategies to mitigate privacy risks were broad, including education, transaction monitoring, and the application of international regulatory models, but were undistinguished from those for non-QTI older adults. Future research should pursue the hypothesis that the risk profile of QTIs may differ in character from that of other older adults, referencing by analogy the nuanced distinctions quantified in credit risk scoring. Such studies would examine the primary data on privacy and data safety implications of hesitant ICT adoption by older adults, using COVID-19 as a natural experiment to identify and evaluate this vulnerable group.
Utility of the Shortness of Breath in Daily Activities Questionnaire (SOBDA-Q) to Detect Sedentary Behavior in Patients with Chronic Obstructive Pulmonary Disease (COPD)
Authors: Yamaji Yoshikazu , Hirano Tsunahiko , Ogawa Hiromasa , Fukatsu-Chikumoto Ayumi , Matsuda Kazuki , Hamada Kazuki , Ohata Shuichiro , Suetake Ryo , Murata Yoriyuki , Oishi Keiji , Asami-Noyama Maki , Edakuni Nobutaka , Kakugawa Tomoyuki , Matsunaga Kazuto
Published: June 2023

Sedentary behavior has been shown to be an independent predictor of mortality in patients with chronic obstructive pulmonary disease (COPD). However, physicians have difficulty ascertaining patients’ activity levels because they tend to avoid shortness of breath. The reformed shortness of breath (SOB) in the daily activities questionnaire (SOBDA-Q) specifies the degree of SOB by measuring low-intensity activity behavior in everyday living. Therefore, we aimed to explore the utility of the SOBDA-Q in detecting sedentary COPD. We compared the modified Medical Research Council dyspnea scale (mMRC), COPD assessment test (CAT), and SOBDA-Q with physical activity levels (PAL) in 17 healthy patients, 32 non-sedentary COPD patients (PAL ≥ 1.5 METs·h), and 15 sedentary COPD patients (PAL < 1.5 METs·h) in this cross-sectional study. CAT and all domains of the SOBDA-Q in all patients are significantly correlated with PAL, even after adjusting for age. The dietary domain has the highest specificity, and the outdoor activity domain has the highest sensitivity for detecting sedentary COPD. Combining these domains helped determine patients with sedentary COPD (AUC = 0.829, sensitivity = 1.00, specificity = 0.55). The SOBDA-Q is associated with PAL and could be a useful tool for determining patients with sedentary COPD. Moreover, eating and outing inactivity claims reflect sedentary behavior in patients with COPD.
Anterior Access to the Cervicothoracic Junction via Partial Sternotomy: A Clinical Series Reporting on Technical Feasibility, Postoperative Morbidity, and Early Surgical Outcome
Authors: Issa Mohammed , Neumann Jan-Oliver , Al-Maisary Sameer , Dyckhoff Gerhard , Kronlage Moritz , Kiening Karl L. , Ishak Basem , Unterberg Andreas W. , Scherer Moritz
Published: June 2023

Surgical access to the cervicothoracic junction (CTJ) is challenging. The aim of this study was to assess technical feasibility, early morbidity, and outcome in patients undergoing anterior access to the CTJ via partial sternotomy. Consecutive cases with CTJ pathology treated via anterior access and partial sternotomy at a single academic center from 2017 to 2022 were retrospectively reviewed. Clinical data, perioperative imaging, and outcome were assessed with regards to the aims of the study. A total of eight cases were analyzed: four (50%) bone metastases, one (12.5%) traumatic instable fracture (B3-AO-Fracture), one (12.5%) thoracic disc herniation with spinal cord compression, and two (25%) infectious pathologic fractures from tuberculosis and spondylodiscitis. The median age was 49.9 years (range: 22–74 y), with a 75% male preponderance. The median Spinal Instability Neoplastic Score (SINS) was 14.5 (IQR: 5; range: 9–16), indicating a high degree of instability in treated cases. Four cases (50%) underwent additional posterior instrumentation. All surgical procedures were performed uneventfully, with no intraoperative complications. The median length of hospital stay was 11.5 days (IQR: 9; range: 6–20), including a median of 1 day in an intensive care unit (ICU). Two cases developed postoperative dysphagia related to stretching and temporary dysfunction of the recurrent laryngeal nerve. Both cases completely recovered at 3 months follow-up. No in-hospital mortality was observed. The radiological outcome was unremarkable in all cases, with no case of implant failure. One case died due to the underlying disease during follow-up. The median follow-up was 2.6 months (IQR: 23.8; range: 1–45.7 months). Our series indicates that the anterior approach to the cervicothoracic junction and upper thoracic spine via partial sternotomy can be considered an effective option for treatment of anterior spinal pathologies, exhibiting a reasonable safety profile. Careful case selection is essential to adequately balance clinical benefits and surgical invasiveness for these procedures.
Prevalence of Temporomandibular Disorders Based on a Shortened Symptom Questionnaire of the Diagnostic Criteria for Temporomandibular Disorders and Its Screening Reliability for Children and Adolescents Aged 7–14 Years
Authors: Rentsch Mathias , Zumbrunn Wojczyńska Aleksandra , Gallo Luigi M. , Colombo Vera
Published: June 2023

The prevalence and adequacy of diagnostic approaches for temporomandibular disorders (TMD) in children and adolescents are still matters of debate. This study aimed to determine the prevalence of TMD and oral habits in children and adolescents aged 7–14 years and evaluate the consistency between self-reported TMD symptoms and clinical findings using a shortened Axis I of Diagnostic Criteria for TMD (DC/TMD). Children (aged 7–10) and adolescents (aged 11–14) of both sexes were invited to participate in this study (n = 1468). Descriptive statistics for all observed variables and Mann–Whitney U-Tests for the clinical examination were performed. A total of 239 subjects participated in the study (response rate 16.3%). The self-reported prevalence of TMD was found to be 18.8%. The most frequently reported oral habit was nail biting (37.7%), followed by clenching (32.2%) and grinding (25.5%). Self-reported headache increased with age, while clenching and grinding decreased. Based on the answers to the DC/TMD Symptom Questionnaire, subgroups of asymptomatic and symptomatic participants (n = 59; 24.7%) were established and randomly selected for the clinical examination (f = 30). The shortened Symptom Questionnaire showed a sensitivity of 0.556 and a specificity of 0.719 for pain during the clinical examination. Although the Symptom Questionnaire exhibited high specificity (0.933), its sensitivity (0.286) for temporomandibular joint sounds was low. Disc displacement with reduction (10.2%) and myalgia (6.8%) were the most common diagnoses. In conclusion, the self-reported prevalence of TMD in children and adolescents in this study was comparable to that reported in the literature for adults. However, the accuracy of the shortened Symptom Questionnaire as a screening tool for TMD-related pain and jaw sounds in children and adolescents was found to be low.
CNS Manifestations in Mucolipidosis Type II—A Retrospective Analysis of Longitudinal Data on Neurocognitive Development and Neuroimaging in Eleven Patients
Authors: Ammer Luise Sophie , Täuber Karolin , Perez Anna , Dohrmann Thorsten , Denecke Jonas , Santer René , Blümlein Ulrike , Ozga Ann-Kathrin , Pohl Sandra , Muschol Nicole Maria
Published: June 2023

Mucolipidosis type II (MLII), an ultra-rare lysosomal storage disorder, manifests as a fatal multi-systemic disease. Mental inhibition and progressive neurodegeneration are commonly reported disease manifestations. Nevertheless, longitudinal data on neurocognitive testing and neuroimaging lack in current literature. This study aimed to provide details on central nervous system manifestations in MLII. All MLII patients with at least one standardized developmental assessment performed between 2005 and 2022 were included by retrospective chart review. A multiple mixed linear regression model was applied. Eleven patients with a median age of 34.0 months (range 1.6–159.6) underwent 32 neurocognitive and 28 adaptive behaviour assessments as well as 14 brain magnetic resonance imagings. The scales used were mainly BSID-III (42%) and VABS-II (47%). Neurocognitive testing (per patient: mean 2.9, standard deviation (SD) 2.0) performed over 0–52.1 months (median 12.1) revealed profound impairment with a mean developmental quotient of 36.7% (SD 20.4) at last assessment. The patients showed sustained development; on average, they gained 0.28 age-equivalent score points per month (confidence interval 0.17–0.38). Apart from common (63%) cervical spinal stenosis, neuroimaging revealed unspecific, non-progressive abnormalities (i.e., mild brain atrophy, white matter lesions). In summary, MLII is associated with profound developmental impairment, but not with neurodegeneration and neurocognitive decline.
Reliability of Universal-Platform-Based Voice Screen Application in AVQI Measurements Captured with Different Smartphones
Authors: Uloza Virgilijus , Ulozaitė-Stanienė Nora , Petrauskas Tadas , Pribuišis Kipras , Blažauskas Tomas , Damaševičius Robertas , Maskeliūnas Rytis
Published: June 2023

The aim of the study was to develop a universal-platform-based (UPB) application suitable for different smartphones for estimation of the Acoustic Voice Quality Index (AVQI) and evaluate its reliability in AVQI measurements and normal and pathological voice differentiation. Our study group consisted of 135 adult individuals, including 49 with normal voices and 86 patients with pathological voices. The developed UPB “Voice Screen” application installed on five iOS and Android smartphones was used for AVQI estimation. The AVQI measures calculated from voice recordings obtained from a reference studio microphone were compared with AVQI results obtained using smartphones. The diagnostic accuracy of differentiating normal and pathological voices was evaluated by applying receiver-operating characteristics. One-way ANOVA analysis did not detect statistically significant differences between mean AVQI scores revealed using a studio microphone and different smartphones (F = 0.759; p = 0.58). Almost perfect direct linear correlations (r = 0.991–0.987) were observed between the AVQI results obtained with a studio microphone and different smartphones. An acceptable level of precision of the AVQI in discriminating between normal and pathological voices was yielded, with areas under the curve (AUC) displaying 0.834–0.862. There were no statistically significant differences between the AUCs (p > 0.05) obtained from studio and smartphones’ microphones. The significant difference revealed between the AUCs was only 0.028. The UPB “Voice Screen” application represented an accurate and robust tool for voice quality measurements and normal vs. pathological voice screening purposes, demonstrating the potential to be used by patients and clinicians for voice assessment, employing both iOS and Android smartphones.
Utility of Cardiac Rehabilitation for Long-Term Outcomes in Patients with Hospital-Acquired Functional Decline after Cardiac Surgery: A Retrospective Study
Authors: Hirakawa Kotaro , Nakayama Atsuko , Hori Kentaro , Uewaki Reina , Shimokawa Tomoki , Isobe Mitsuaki
Published: June 2023

Hospital-acquired functional decline is an important outcome that affects the long-term prognosis of patients after cardiac surgery. Phase II cardiac rehabilitation (CR) for outpatients is expected to improve prognosis; however, this is not clear in patients with hospital-acquired functional decline after cardiac surgery. Therefore, this study evaluated whether phase II CR improved the long-term prognosis of patients with hospital-acquired functional decline after cardiac surgery. This single-center, retrospective observational study included 2371 patients who required cardiac surgery. Hospital-acquired functional decline occurred in 377 patients (15.9%) after cardiac surgery. The mean follow-up period was 1219 ± 682 days in all patients, and there were 221 (9.3%) cases with major adverse cardiovascular events (MACE) after discharge during the follow-up period. The Kaplan–Meier survival curves indicated that hospital-acquired functional decline and non-phase II CR was associated with a higher incidence of MACE than other groups (log-rank, p < 0.001), additionally exhibiting prognosticating MACE in multivariate Cox regression analysis (HR, 1.59; 95% CI, 1.01–2.50; p = 0.047). Hospital-acquired functional decline after cardiac surgery and non-phase II CR were risk factors for MACE. The participation in phase II CR in patients with hospital-acquired functional decline after cardiac surgery could reduce the risk of MACE.
Lung Large Cell Neuroendocrine Carcinoma: A Population-Based Retrospective Cohort Study
Authors: Mu Xiaoli , Pu Dan , Zhu Yajuan , Zhou Yixin , Wu Qiang , Liu Qing , Yin Liyuan , Li Yan
Published: June 2023

Backgrounds: Pulmonary large cell neuroendocrine carcinoma (LCNEC) is a rarely high-grade neuroendocrine carcinoma of the lung with features of both small cell and non-small cell lung cancer. In this study, we aim to construct a prognostic nomogram that integrates the clinical features and treatment options to predict disease-specific survival (DSS). Methods: A total of 713 patients diagnosed with LCNEC were from the US National Cancer Institute’s Surveillance Epidemiology and End Results (SEER) registry between 2010–2016. Cox proportional hazards analysis was conducted to choose the significant predictors of DSS. External validation was performed using 77 patients with LCNEC in the West China Hospital Sichuan University between 2010–2018. The predictive accuracy and discriminative capability were estimated by the concordance index (C-index), calibration curve, and receiver operating characteristic (ROC) curve. The clinical applicability of the nomogram was verified through the decision curve analysis (DCA). Additionally, we conducted a subgroup analysis of data available in the external cohort that may impact prognosis but was not recorded in the SEER database. Results: Six independent risk factors for DSS were identified and integrated into the nomogram. The nomogram achieved good C- indexes of 0.803 and 0.767 in the training and validation group, respectively. Moreover, the calibration curves for the probability of survival showed good agreement between prediction by nomogram and actual observation in 1-, 3- and 5-year DSS. The ROC curves demonstrated the prediction accuracy of the established nomogram (all Area Under Curve (AUC) > 0.8). DCA exhibited the favorable clinical applicability of the nomogram in the prediction of LCNEC survival. A risk classification system was built which could perfectly classify LCNEC patients into high-, medium- and low-risk groups (p < 0.001). The survival analysis conducted on the West China Hospital cohort indicated that whole brain radiation therapy (WBRT), prophylactic cranial irradiation (PCI), surgical procedures, tumor grade, Ki-67, and PD-L1 expression were not significantly associated with DSS. Conclusion: This study has effectively developed a prognostic nomogram and a corresponding risk stratification system, which demonstrate promising potential for predicting the DSS of patients with LCNEC.
Evaluating the Natural History of Groin Hernia from an “Unplanned” Watchful Waiting Strategy
Authors: Ceresoli Marco , Adjei Antwi Stella Konadu , Mehmeti Megi , Marmaggi Serena , Braga Marco , Nespoli Luca
Published: June 2023

Groin hernia is one of the most common surgical diagnoses worldwide. The indication for surgery in asymptomatic or mildly symptomatic patients is discussed. Some trials have demonstrated the safety of a watchful waiting strategy. During the pandemic, waiting lists for hernia surgery dramatically increased the opportunity to evaluate the natural history of groin hernias. The present study aimed to evaluate the incidence of emergency hernia surgery in a large cohort of patients that were selected and were waiting for elective surgery. This is a retrospective cross-sectional cohort study including all patients evaluated and selected for elective groin hernia surgery at San Gerardo Hospital between 2017 and 2020. Elective and emergency hernia surgeries were recorded for all patients. The incidence of adverse events was also evaluated. Overall, 1423 patients were evaluated, and 964 selected patients (80.3%) underwent elective hernia surgery, while 17 patients (1.4%) required an emergency operation while waiting for an elective operation. A total of 220 (18.3%) patients were still awaiting surgery in March 2022. The overall cumulative risk levels for emergency hernia surgeries were 1%, 2%, 3.2%, and 5% at 12, 24, 36, and 48 months, respectively. There was no association between longer waiting periods and an increased need for emergency surgery. Our study indicates that up to 5% of patients with groin hernia require emergency surgery at 48 months from the evaluation; the increased waiting time for surgery for elective groin hernia repair was not associated with an increased incidence of adverse events.
Hepatic Vein-Guided Approach in Laparoscopic Anatomic Liver Resection of the Ventral and Dorsal Parts of Segment 8
Authors: Monden Kazuteru , Sadamori Hiroshi , Iwasaki Toshimitsu , Hioki Masayoshi , Takakura Norihisa
Published: June 2023

Laparoscopic ventral and dorsal segmentectomies 8 are an option for parenchymal-sparing liver resection. However, laparoscopic anatomic posterosuperior liver segment resection is technically demanding because of its deep location and the many variations in the segment 8 Glissonean pedicle (G8). In this study, we describe a hepatic vein-guided approach (HVGA) to overcome these limitations. For ventral segmentectomy 8, liver parenchymal transection was initiated at the ventral side of the middle hepatic vein (MHV) and continued exposing it toward the periphery. The G8 ventral branch (G8vent) was identified on the right side of the MHV. Following G8vent dissection, liver parenchymal transection was completed by connecting the demarcation line and G8vent stump. For dorsal segmentectomy 8, the anterior fissure vein (AFV) was exposed peripherally. The G8 dorsal branch (G8dor) was identified on the right side of the AFV. Following G8dor dissection, the right hepatic vein (RHV) was exposed from the root. Liver parenchymal transection was completed by connecting the demarcation line and RHV. Between April 2016 and December 2022, we performed laparoscopic ventral and dorsal segmentectomy 8 in fourteen patients. No complications (Clavien–Dindo classification, Grade ≥ IIIa) were observed. An HVGA is feasible and useful for standardizing safe laparoscopic ventral and dorsal segmentectomies 8.
Effect of Arabic Qahwa on Blood Pressure in Patients with Stage One Hypertension in the Eastern Region of Saudi Arabia
Authors: Jalal Sahbanathul Missiriya , Alsebeiy Saad Hamoud , Aleid Hamida Ali , Alhamad Sukinah Ali
Published: June 2023

Hypertension (HT), which is a condition of increased blood pressure (BP), is a major health problem globally. In Saudi Arabia, morbidity and mortality rates are increasing due to HT. Arabic Qahwa (AQ) is a commonly consumed beverage in Saudi Arabia and has numerous health benefits. We conducted a randomized control trial to investigate the effect of AQ on BP among patients with HT (Stage 1). Based on the inclusion criteria, 140 patients were randomly selected, and 126 patients were followed up. After obtaining demographic information, we assessed the BP, heart rate, and lipid profile before and after the intervention of consuming four cups of AQ daily for four weeks. A paired ‘t’ test was used with a significance level of 5%. In the AQ group, there were significant changes (p = 0.009) in systolic blood pressure (SBP), with means of 134.72 ± 3.23 and 133.14 ± 3.69 observed pre and post-test, respectively. Similarly, the diastolic blood pressure (DBP) pre- and post-test mean scores were 87.08 ± 1.8 and 85.98 ± 1.95, respectively, which also showed significance (p = 0.001). There were also significant changes (p = 0.001) observed in the lipid profile of the AQ group. In conclusion, AQ is effective in reducing SBP and DBP in patients with stage one HT.
Changes in the Spatial Distribution of the Employed Population in the Yangtze River Delta Region since the 21st Century: An Analysis and Discussion Based on Census Data
Authors: Chen Chen
Published: June 2023

Focusing on the Yangtze River Delta region, the spatial distribution and change characteristics of the employed population were assessed by selecting three time points: 2000, 2010 and 2020. Firstly, a correlation was established between population employment statistics and spatial units of administrative divisions to analyze the spatial distribution characteristics of the employed population in general and by industry; secondly, the changing characteristics of the spatial distribution of the employed population over time, including the migration of the centroid and density changes, were analyzed; thirdly, a systematic clustering approach was adopted to carry out a typological analysis of 41 cities in the Yangtze River Delta from three perspectives: industrial structure, time stage and spatial level. It was found that (1) regional differences within the Yangtze River Delta are still significant, but are narrowing; (2) different cities or regions show different characteristics of development stages, and late-developing regions can learn from early developing regions; (3) metropolitan areas are still the main areas of employment concentration, and the spatial distribution of employment in some cities is beginning to suburbanize.
Recent Reports on Bioactive Compounds from Marine Cyanobacteria in Relation to Human Health Applications
Authors: Perera R. M. T. D. , Herath K. H. I. N. M. , Sanjeewa K. K. Asanka , Jayawardena Thilina U.
Published: June 2023

The ocean is a valuable natural resource that contains numerous biologically active compounds with various bioactivities. The marine environment comprises unexplored sources that can be utilized to isolate novel compounds with bioactive properties. Marine cyanobacteria are an excellent source of bioactive compounds that have applications in human health, biofuel, cosmetics, and bioremediation. These cyanobacteria exhibit bioactive properties such as anti-inflammatory, anti-cancer, anti-bacterial, anti-parasitic, anti-diabetic, anti-viral, antioxidant, anti-aging, and anti-obesity effects, making them promising candidates for drug development. In recent decades, researchers have focused on isolating novel bioactive compounds from different marine cyanobacteria species for the development of therapeutics for various diseases that affect human health. This review provides an update on recent studies that explore the bioactive properties of marine cyanobacteria, with a particular focus on their potential use in human health applications.
Application of Load-Sharing Concept to Mechanical Seals
Authors: Rahimpour Mohsen , Samadani Alireza , Akbarzadeh Saleh
Published: June 2023

Mechanical seals are mechanisms that are used to prevent fluid leakage. Since the seal surfaces are in contact with one another, hydrodynamic and contact forces are functions of surface roughness. Additionally, since the lubrication regime under specific operating conditions such as low speed or high load causes the seal to operate in the mixed lubrication regime, thus the contact of asperities plays an important role. The primary purpose of this paper is to apply the load-sharing concept to study the behavior of a mechanical seal in a mixed lubrication regime. The predicted results are compared to the published data from the literature, showing acceptable accuracy. The model presented in this paper can predict the performance of the mechanical seal system in a short execution time while providing acceptable accuracy by considering the surface roughness effect.
Development and Promotion of Concrete Strength at Initial 24 Hours
Authors: Fan Chuanhe , Qian Jueshi , Sun Huaqiang , Fan Yingru
Published: June 2023

Knowing and promoting the strength development of concrete at an earlier age is essential for accelerating formwork circulation of the on-site construction and precast product manufacture. The strength development rate at earlier ages of less than the initial 24 h was investigated. The effect of measures of adding silica fume, calcium sulfoaluminate cement, and early strength agent on the strength development of earlier concrete at ambient temperatures of 10, 15, 20, 25, and 30 °C was studied. The microstructure and long-term properties were further tested. It is shown that the strength increases exponentially first and then logarithmically, different from what is commonly recognized. Increasing cement content exhibited a certain effect only above 25 °C. When the cement content increased from 420 to 460 kg/m3, the strength only increased from 6.2 to 6.7 MPa after 12 h at 25 °C. The early strength agent could increase the strength significantly, the strength could be increased from 6.4 to 10.8 MPa after 20 h at 10 °C and from 7.2 to 20.6 MPa after 14 h at 20 °C. All measures for promoting earlier strength did not have an evident negative effect. The results could be potentially referred for the formwork removal at a suitable moment.
A Study on the Fatigue Performance and Corrosion Resistance of 304/45 Bimetallic Composite Bolts
Authors: Zhou Ziming , Ding Yi
Published: June 2023

This paper utilized a hot-rolling process to produce composite rods and subsequently manufactured 304/45 composite bolts through the process of drawing and thread rolling. The study focused on examining the microstructure, fatigue performance, and corrosion resistance of these composite bolts. Additionally, the impacts of quenching and tempering on the fatigue performance of the composite bolts were explored and compared to the performance of 304 stainless steel (SS) bolts and Grade 6.8 35K carbon steel (CS) bolts. The results indicate that the SS cladding of the cold-worked 304/45 composite (304/45-CW) bolts was primarily strengthened by the cold deformation mechanism, which resulted in high microhardness, averaging 474 HV. At a maximum surface bending stress of 300 MPa, the fatigue cycles of the 304/45-CW reached 342,600 cycles at a 63.2% failure probability, which was significantly higher than that of commercial 35K CS bolts. The S-N fatigue curves showed that the fatigue strength of the 304/45-CW bolts was approximately 240 MPa, but the fatigue strength of the quenched and tempered 304/45 composite (304/45-QT) bolts decreased significantly to 85 MPa, due to the loss of the cold deformation strengthening effect. The corrosion resistance of the SS cladding of the 304/45-CW bolts was impressive and remained largely unaffected by carbon element diffusion.