bluuebunny/crossref_metadata_2025 huggingface dataset:
Metabolic Unhealthiness Increases the Likelihood of Having Metabolic Syndrome Components in Normoweight Young Adults
Authors: Bintoro Bagas , Fan Yen-Chun , Chou Chia-Chi , Chien Kuo-Liong , Bai Chyi-Huey
Published: September 2019

Findings on risk detection for having metabolic syndrome (MetS) components, each of which may individually increase the risk of disease and mortality, are limited in young adults. In this study, we aimed to calculate the likelihood of having ≥1 MetS component in normoweight young adults using two different metabolic health criteria. We recruited 1182 normoweight young adults from the Taiwan Survey on the Prevalence of Hypertension, Hyperglycemia, and Hyperlipidemia and the National Health Interview Survey (aged 16–45 years, 39% male, body mass index = 18.5–22.99, all without MetS) and followed them for 5 years. Metabolic health criteria were derived from the Harmonized criteria (unhealthy if showing abnormality in one or two MetS components) and the triglyceride-glucose index (TyG-i; unhealthy if TyG-i was in the >75th percentile). Odds ratio (OR) and 95% confidence interval (CI) estimations for having ≥1 MetS component and for having each MetS component in 5 years were assessed using multivariable-adjusted logistic regression. We observed a significantly increased likelihood of the presence of ≥1 MetS component in the unhealthy group by using the Harmonized criteria and TyG-i (adjusted OR (aOR); 95%CI: 2.64; 2.02-3.45 and 2.1; 1.57–2.82, respectively). The areas under the receiver-operating characteristics curves were 0.679 and 0.652 for the final models using Harmonized and TyG-i criteria, respectively. These findings support the recommendation of treating any metabolic component abnormality, even in young adults without a MetS diagnosis.
Cold Weather Conditions and Risk of Hypothermia Among People Experiencing Homelessness: Implications for Prevention Strategies
Authors: Zhang Paige , Wiens Kathryn , Wang Ri , Luong Linh , Ansara Donna , Gower Stephanie , Bassil Kate , Hwang Stephen W.
Published: September 2019

Hypothermia is a preventable condition that disproportionately affects individuals who experience homelessness, yet limited data exist to inform the response to cold weather. To fill this gap, we examined the association between meteorological conditions and the risk of hypothermia among homeless individuals. Hypothermic events were identified from emergency department charts and coroner’s records between 2004 and 2015 in Toronto, Canada. A time-stratified case-crossover design with conditional logistic regression was used to assess the relationship between the meteorological conditions (minimum temperature and precipitation) and the risk of hypothermia. There were 97 hypothermic events identified: 79 injuries and 18 deaths. The odds of experiencing a hypothermic event increased 1.64-fold (95% CI: 1.30–2.07) with every 5 °C decrease in the minimum daily temperature and 1.10-fold (95% CI: 1.03–1.17) with every 1 mm increase in precipitation. The risk of hypothermia among individuals experiencing homelessness increased with declining temperature; however, most cases occurred during periods of low and moderate cold stress. 72% occurred when the minimum daily temperatures were warmer than −15 °C. These findings highlight the importance of providing a seasonal cold weather response to prevent hypothermia, complemented by an alert-based response on extremely cold days.
Molecular Characteristics and Treatment of Endothelial Dysfunction in Patients with COPD: A Review Article
Authors: Szucs Botond , Szucs Csilla , Petrekanits Mate , Varga Janos T.
Published: September 2019

Patients with chronic obstructive pulmonary disease (COPD) show systemic consequences, such as chronic systemic inflammation leading to changes in the airway, airway penetrability, and endothelial function. Endothelial dysfunction is characterized by a list of alterations of endothelium towards reduced vasodilation, proinflammatory state, detachment and apoptosis of endothelial cells, and development of atherosclerosis. COPD-induced endothelial dysfunction is associated with elevated cardiovascular risk. The increment of physical activities such as pulmonary rehabilitation (PR) training have a significant effect on COPD, thus, PR can be an integrative part of COPD treatment. In this narrative review the focus is on the function of endothelial inflammatory mediators [cytokines, chemokines, and cellular proteases] and pulmonary endothelial cells and endothelial dysfunction in COPD as well as the effects of dysfunction of the endothelium may play in COPD-related pulmonary hypertension. The relationship between smoking and endothelial dysfunction is also discussed. The connection between different pulmonary rehabilitation programs, arterial stiffness and pulse wave velocity (PWV) is presented. Endothelial dysfunction is a significant prognostic factor of COPD, which can be characterized by PWV. We discuss future considerations, like training programs, as an important part of the treatment that has a favorable impact on the endothelial function.
Molecular Docking: Shifting Paradigms in Drug Discovery
Authors: Pinzi Luca , Rastelli Giulio
Published: September 2019

Molecular docking is an established in silico structure-based method widely used in drug discovery. Docking enables the identification of novel compounds of therapeutic interest, predicting ligand-target interactions at a molecular level, or delineating structure-activity relationships (SAR), without knowing a priori the chemical structure of other target modulators. Although it was originally developed to help understanding the mechanisms of molecular recognition between small and large molecules, uses and applications of docking in drug discovery have heavily changed over the last years. In this review, we describe how molecular docking was firstly applied to assist in drug discovery tasks. Then, we illustrate newer and emergent uses and applications of docking, including prediction of adverse effects, polypharmacology, drug repurposing, and target fishing and profiling, discussing also future applications and further potential of this technique when combined with emergent techniques, such as artificial intelligence.
Exosomes in Systemic Sclerosis: Messengers Between Immune, Vascular and Fibrotic Components?
Authors: Colletti Marta , Galardi Angela , De Santis Maria , Guidelli Giacomo Maria , Di Giannatale Angela , Di Luigi Luigi , Antinozzi Cristina
Published: September 2019

Systemic sclerosis (SSc) is a rare autoimmune disease, characterized by vasculopathy and fibrosis of the skin and internal organs. This disease is still considered incurable and is associated with a high risk of mortality, which is related to fibrotic events. An early diagnosis is useful for preventing complications, and targeted therapies reduce disease progression and ameliorate patients’ quality of life. Nevertheless, there are no validated biomarkers for early diagnosis with predictive prognostic value. Exosomes are membrane vesicles, transporting proteins and nucleic acids that may be delivered to target cells, which influences cellular behavior. They play important roles in cell–cell communication, both in physiological and pathological conditions, and may be useful as circulating biomarkers. Recent evidences suggest a role for these microvesicles in the three main aspects related to the pathogenesis of SSc (immunity, vascular damage, and fibrosis). Moreover, exosomes are of particular interest in the field of nano-delivery and are used as biological carriers. In this review, we report the latest information concerning SSc pathogenesis, clinical aspects of SSc, and current approaches to the treatment of SSc. Furthermore, we indicate a possible role of exosomes in SSc pathogenesis and suggest their potential use as diagnostic and prognostic biomarkers, as well as therapeutic tools.
Inhibition of Tyrosine-Phosphorylated STAT3 in Human Breast and Lung Cancer Cells by Manuka Honey is Mediated by Selective Antagonism of the IL-6 Receptor
Authors: Aryappalli Priyanka , Shabbiri Khadija , Masad Razan J. , Al-Marri Roadha H. , Haneefa Shoja M. , Mohamed Yassir A. , Arafat Kholoud , Attoub Samir , Cabral-Marques Otavio , Ramadi Khalil B. , Fernandez-Cabezudo Maria J. , al-Ramadi Basel K.
Published: September 2019

Aberrantly high levels of tyrosine-phosphorylated signal transducer and activator of transcription 3 (p-STAT3) are found constitutively in ~50% of human lung and breast cancers, acting as an oncogenic transcription factor. We previously demonstrated that Manuka honey (MH) inhibits p-STAT3 in breast cancer cells, but the exact mechanism remained unknown. Herein, we show that MH-mediated inhibition of p-STAT3 in breast (MDA-MB-231) and lung (A549) cancer cell lines is accompanied by decreased levels of gp130 and p-JAK2, two upstream components of the IL-6 receptor (IL-6R) signaling pathway. Using an ELISA-based assay, we demonstrate that MH binds directly to IL-6Rα, significantly inhibiting (~60%) its binding to the IL-6 ligand. Importantly, no evidence of MH binding to two other cytokine receptors, IL-11Rα and IL-8R, was found. Moreover, MH did not alter the levels of tyrosine-phosphorylated or total Src family kinases, which are also constitutively activated in cancer cells, suggesting that signaling via other growth factor receptors is unaffected by MH. Binding of five major MH flavonoids (luteolin, quercetin, galangin, pinocembrin, and chrysin) was also tested, and all but pinocembrin could demonstrably bind IL-6Rα, partially (30–35%) blocking IL-6 binding at the highest concentration (50 μM) used. In agreement, each flavonoid inhibited p-STAT3 in a dose-dependent manner, with estimated IC50 values in the 3.5–70 μM range. Finally, docking analysis confirmed the capacity of each flavonoid to bind in an energetically favorable configuration to IL-6Rα at a site predicted to interfere with ligand binding. Taken together, our findings identify IL-6Rα as a direct target of MH and its flavonoids, highlighting IL-6R blockade as a mechanism for the anti-tumor activity of MH, as well as a viable therapeutic target in IL-6-dependent cancers.
The Outcomes of Pancreatic Transplantation from Pediatric Donors–A Single Institution Experience
Authors: Ito Taihei , Kenmochi Takashi , Aida Naohiro , Kurihara Kei , Kawai Akihiro , Suzuki Atsushi , Shibata Megumi , Hiratsuka Izumi , Hasegawa Midori
Published: September 2019

Objectives: The aim of this study was to compare the outcomes of pancreatic transplantation from pediatric donors younger than 15 years of age to the outcomes of pancreatic transplantation from adult donors. Methods: Sixty patients underwent pancreatic transplantation in our facility from August 2012 to June 2019. These patients were divided into two groups according to the age of the donor: Cases in which the donor was younger than 15 years of age were classified into the PD group (n = 7), while those in which the donor was older than 15 years of age were classified into the AD group (n = 53). The outcomes of pancreas transplantation were retrospectively compared between the two groups. Results: Pancreatic graft survival did not differ between the PD and AD groups. Furthermore, there were no differences in the HbA1c and serum creatinine levels at three months, with good values maintained in both groups. The results of oral glucose tolerance tests (OGTTs) revealed that the blood glucose concentration did not differ between the two groups. However, the serum insulin concentration at 30 min after 75 g glucose loading was significantly higher in the PD group. Conclusion: The outcomes of pancreatic transplantation from pediatric donors may be comparable to those of pancreatic transplantation from adult donors and the insulin secretion ability after transplantation may be better.
Critical Processes of Trace Metals Mobility in Transitional Waters: Implications from the Remote, Antinioti Lagoon, Corfu Island, Greece
Authors: Botsou Fotini , Karageorgis Aristomenis P. , Paraskevopoulou Vasiliki , Dassenakis Manos , Scoullos Michael
Published: September 2019

The Antinioti Lagoon is a karstified, rather pristine, and shallow coastal lagoon located in the northern part of Corfu Island in NW Greece. The present study examines the levels of metals (Al, Fe, Mn, Cd, Cu, Pb, and Zn) in the dissolved and particulate phase, as well as in surface and core sediments, and identifies the critical processes that define their behavior. The major transport pathway of dissolved Mn, Cd, and Pb, and particulate Mn, Cd, and Zn into the lagoon is through freshwater springs, whereas surface runoff dominates the transport of particulate Al, Fe, and Cu. Interestingly, large particles (>8 μm) contain higher amounts of Al, Fe and Mn than the finer ones (<8 μm), due to flocculation of oxyhydroxides that, eventually, scavenge other metals, as well. Cadmium and Zn bound to the large particles were found to be less prone to desorption than the smaller ones and were effectively captured within the lagoon. In the sediments, diagenetic processes are responsible for post-depositional changes in the forms of metals (particularly Fe, Mn and Cd). Enrichment factors (EFs) based on local background showed that sediments are enriched in restricted areas in Cd and Pb by maximum factors 4.8 and 10, respectively. These metals were predominantly found in potentially labile forms. Thus, any interventions introducing changes in the physico-chemical conditions may result in the release of metals, with negative implications on the lagoon’s ecological quality.
The Influence of Lignin Diversity on the Structural and Thermal Properties of Polymeric Microspheres Derived from Lignin, Styrene, and/or Divinylbenzene
Authors: Goliszek Marta , Podkościelna Beata , Sevastyanova Olena , Gawdzik Barbara , Chabros Artur
Published: September 2019

This work investigates the impact of lignin origin and structural characteristics, such as molecular weight and functionality, on the properties of corresponding porous biopolymeric microspheres obtained through suspension-emulsion polymerization of lignin with styrene (St) and/or divinylbenzene (DVB). Two types of kraft lignin, which are softwood (Picea abies L.) and hardwood (Eucalyptus grandis), fractionated by common industrial solvents, and related methacrylates, were used in the synthesis. The presence of the appropriate functional groups in the lignins and in the corresponding microspheres were investigated by attenuated total reflectance Fourier transform infrared spectroscopy (ATR/FT-IR), while the thermal properties were studied by differential scanning calorimetry (DSC). The texture of the microspheres was characterized using low-temperature nitrogen adsorption. The swelling studies were performed in typical organic solvents and distilled water. The shapes of the microspheres were confirmed with an optical microscope. The introduction of lignin into a St and/or DVB polymeric system made it possible to obtain highly porous functionalized microspheres that increase their sorption potential. Lignin methacrylates created a polymer network with St and DVB, whereas the unmodified lignin acted mainly as an eco-friendly filler in the pores of St-DVB or DVB microspheres. The incorporation of biopolymer into the microspheres could be a promising alternative to a modification of synthetic materials and a better utilization of lignin.
Micromechanical Modelling of the Influence of Strain Ratio on Fatigue Crack Initiation in a Martensitic Steel-A Comparison of Different Fatigue Indicator Parameters
Authors: Schäfer Benjamin Josef , Sonnweber-Ribic Petra , ul Hassan Hamad , Hartmaier Alexander
Published: September 2019

Micromechanical fatigue lifetime predictions, in particular for the high cycle fatigue regime, require an appropriate modelling of mean stress effects in order to account for lifetime reducing positive mean stresses. Focus of this micromechanical study is the comparison of three selected fatigue indicator parameters (FIPs), with respect to their applicability to different total strain ratios. In this work, investigations are performed on the modelling and prediction of the fatigue crack initiation life of the martensitic high-strength steel SAE 4150 for two different total strain ratios. First, multiple martensitic statistical volume elements (SVEs) are generated by multiscale Voronoi tessellations. Micromechanical fatigue simulations are then performed on these SVEs by means of a crystal plasticity model to obtain microstructure dependent fatigue responses. In order to account for the material specific fatigue damage zone, a non-local homogenisation scheme for the FIPs is introduced for lath martensitic microstructures. The numerical results of the different non-local FIPs are compared with experimental fatigue crack initiation results for two different total strain ratios. It is concluded that the multiaxial fatigue criteria proposed by Fatemi-Socie is superior for predicting fatigue crack initiation life to the energy dissipation criteria and the accumulated plastic slip criteria for the investigated total strain ratios.
Influence of the Ni/Co Mass Ratio on the Microstructure and Properties of Quaternary Cu-Ni-Co-Si Alloys
Authors: Li Jiang , Huang Guojie , Mi Xujun , Peng Lijun , Xie Haofeng , Kang Yonglin
Published: September 2019

The properties and microstructural evolution of quaternary Cu-Ni-Co-Si alloys with different Ni/Co mass ratios are investigated systematically. These alloys exhibit higher mechanical properties when the Ni/Co mass ratio is 1.12-1.95 (NC-4-NC-5) and show excellent electrical conductivity when the Ni/Co mass ratio is 0.05-0.5 (NC-1-NC-3). With an increase in the Ni/Co ratio, the dimension of precipitated phase continues to increase and the grain size also visibly grows and coarsens. At the same time, the precipitation process of the NC-5 alloy is the most adequate, resulting in the highest mechanical properties. In addition, the precipitated phase in the alloys was confirmed to be the (Ni, Co)2Si composite phase. The number of Ni2Si phases in the precipitated phase gradually increased, and the Ni atoms exhibited the strongest co-segregation alongside the increasing Ni/Co ratio. Compared with the alloy without a Co element, the addition of Co helped refine the grain size and accelerate the precipitation of the particle phase and purify solute atoms in the matrix, thereby simultaneously improving mechanical properties and conductivity. The present work provides a new method for the development of multicomponent Cu-Ni-Si-Co-X alloys with outstanding comprehensive performance.
The Triple Mechanisms of Atenolol Adsorption on Ca-Montmorillonite: Implication in Pharmaceutical Wastewater Treatment
Authors: Chang Po-Hsiang , Jiang Wei-Teh , Sarkar Binoy , Wang Wendong , Li Zhaohui
Published: September 2019

The adsorption of atenolol (AT) from aqueous solutions by Ca-montmorillonite (SAz-2) was investigated in batch studies under different physicochemical conditions. The AT existed in neutral un-dissociated form at pH 10, and was adsorbed on dioctahedral smectite (SAz-2) obeying the Langmuir isotherm with a maximum adsorption capacity of 330 mmol/kg. The kinetic adsorption suggested that both strong and weak adsorption sites existed on SAz-2 and participated in the adsorption mechanisms. The amount of exchangeable cations desorbed from SAz-2 during AT adsorption was linearly correlated with the amounts of adsorbed AT having slopes of 0.43, which implied that a cation exchange based adsorption mechanism was also in place. A comprehensive basal spacing change of SAz-2 was observed after AT adsorption on the clay mineral when tested with or without AT recrystallization. The intercalation of AT into the SAz-2 interlayers did not result in swelling due to the low adsorption capacity of the drug. Prominent interactions between the pharmaceutical molecule and SAz-2 were evidenced by apparent shifts of the infrared absorption bands after adsorption. The interlayer configurations and hydrogen bonding of AT on SAz-2 were also supported by infrared, X-ray diffraction and thermogravimetric analyses. This study suggested that SAz-2 is an excellent material to remove not only AT from pharmaceutical wastewater, but can potentially remove many other β-receptor blocker drugs. The results helped us to understand the possible interlayer configurations and adsorption mechanisms of the drugs on natural clay mineral based adsorbents.
N-Terminal Acetylation and C-Terminal Amidation of Spirulina platensis-Derived Hexapeptide: Anti-Photoaging Activity and Proteomic Analysis
Authors: Zeng Qiaohui , Jiang Jianguo , Wang Jingjing , Zhou Qiuchan , Zhang Xuewu
Published: September 2019

Ultraviolet (UV) irradiation is a potent inducer for skin photoaging. This paper investigated the anti-photoaging effects of the acetylated and amidated hexapeptide (AAH), originally identified from Spirulina platensis, in (Ultraviolet B) UVB-irradiated Human immortalized keratinocytes (Hacats) and mice. The results demonstrated that AAH had much lower toxicity on Hacats than the positive matrixyl (81.52% vs. 5.32%). Moreover, AAH reduced MDA content by 49%; increased SOD, CAT, and GSH-Px activities by 103%, 49%, and 116%, respectively; decreased MMP-1 and MMP-3 expressions by 27% and 29%, respectively, compared to UVB-irradiated mice. Employing isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomics, 60 differential proteins were identified, and major metabolic pathways were determined. Network analysis indicated that these differential proteins were mapped into an interaction network composed of two core sub-networks. Collectively, AAH is protective against UVB-induced skin photoaging and has potential application in skin care cosmetics.
Steel/Slag Interface Behavior under Multifunction Electromagnetic Driving in a Continuous Casting Slab Mold
Authors: Sun Xiaohui , Li Bin , Lu Haibiao , Zhong Yunbo , Ren Zhongming , Lei Zuosheng
Published: September 2019

The transient numerical model combined with the volume of fluid (VOF) approach is employed to investigate the steel/slag interface behavior under multifunction electromagnetic driving in a continuous casting slab mold. Here, electromagnetic stirring (EMS) and electromagnetic braking (EMBr), respectively, are chosen as flow multifunction control technologies in the upper and lower areas of the mold. The computational models are validated with measurement results. The results show that multifunction electromagnetic driving changes the flow pattern, which has the potential to simultaneously meet the requirements of the steel flow in the regions above and below the nozzle, ensuring the uniformity and activity of the molten steel in the upper region of the mold and avoiding the excessive depth of the impinging jet. After EMS, the steel forms a deflected circulation flow at the steel/slag interface, and the surface velocity distribution is more uniform. EMBr still has the function of stabilizing the meniscus when multifunction electromagnetic driving is applied. Taking wave height and wave amplitude as evaluation criteria, the influence of EMS and EMBr on the steel/slag interface can be evaluated and controlled to some extent by observing the key points.
A Nontoxic Battery with 3D-Printed Housing for On-Demand Operation of Microcontrollers in Microfluidic Sensors
Authors: Sachsenheimer Kai , Richter Christiane , Helmer Dorothea , Kotz Frederik , Rapp Bastian Ernst
Published: September 2019

Microcontrollers have a low energy consumption and are convenient tools for the operation and readout of small lab-on-a-chip devices. The operation of microcontrollers for data collection and analysis is key for measurements and statistics in field experiments. However, for portable lab-on-a-chip or point-of-care systems in low-resource settings, the availability of energy sources is a bottleneck. Here, we present a simple, nontoxic aluminum/air redox battery with a 3D-printed housing for on-demand operation of a sensor using a microcontroller for data collection. The battery is stored in a dry state and can be manufactured conveniently using off-the-shelf components and a simple 3D printer. It can be quickly assembled and operates a microcontroller for at least one hour in continuous operation mode. We demonstrate its performance by collecting data from a capacitive sensor capable of determining the conductivity of liquid samples. Such sensors can be used for, e.g., determining the water quality or phase formation in liquid mixtures. The sensor performance in determining different conductivities of nonconductive and conductive liquids in droplets is demonstrated.
Rhodotorula mucilaginosa C2.5t1 Modulates Carotenoid Content and CAR Genes Transcript Levels to Counteract the Pro-Oxidant Effect of Hydrogen Peroxide
Authors: Landolfo Sara , Chessa Rossella , Zara Giacomo , Zara Severino , Budroni Marilena , Mannazzu Ilaria
Published: September 2019

In order to contribute to the elucidation of the biological role of carotenoids, the cellular response to hydrogen peroxide was analyzed in the red yeast R. mucilaginosa. For that, the wild strain C2.5t1, that produces β-carotene, torulene, and torularhodin, and the albino mutant 200A6 that is incapable of producing detectable amounts of these carotenoids, were grown in the presence of increasing concentrations of hydrogen peroxide. In spite of the difference in carotenoid content, the two strains presented comparable resistance to the pro-oxidant that showed a minimum inhibitory concentration of 6 mM. When subject to 1 h treatment with 16 mM hydrogen peroxide the two strains increased catalase but not superoxide activity, suggesting that catalase plays a major role in cell protection in both the wild strain and the albino mutant. Moreover, C2.5t1 reduced its carotenoid content by about 40% upon hydrogen peroxide treatment. This reduction in carotenoids was in agreement with a significant decrease of the transcript levels of genes involved in carotenoid biosynthesis. Since an excess of β-carotene may enhance reactive oxygen species toxicity, these results suggest that C2.5t1 modulates carotenoid content to counteract the pro-oxidant effect of hydrogen peroxide.
The Emerging Role of l-Glutamine in Cardiovascular Health and Disease
Authors: Durante William
Published: September 2019

Emerging evidence indicates that l-glutamine (Gln) plays a fundamental role in cardiovascular physiology and pathology. By serving as a substrate for the synthesis of DNA, ATP, proteins, and lipids, Gln drives critical processes in vascular cells, including proliferation, migration, apoptosis, senescence, and extracellular matrix deposition. Furthermore, Gln exerts potent antioxidant and anti-inflammatory effects in the circulation by inducing the expression of heme oxygenase-1, heat shock proteins, and glutathione. Gln also promotes cardiovascular health by serving as an l-arginine precursor to optimize nitric oxide synthesis. Importantly, Gln mitigates numerous risk factors for cardiovascular disease, such as hypertension, hyperlipidemia, glucose intolerance, obesity, and diabetes. Many studies demonstrate that Gln supplementation protects against cardiometabolic disease, ischemia-reperfusion injury, sickle cell disease, cardiac injury by inimical stimuli, and may be beneficial in patients with heart failure. However, excessive shunting of Gln to the Krebs cycle can precipitate aberrant angiogenic responses and the development of pulmonary arterial hypertension. In these instances, therapeutic targeting of the enzymes involved in glutaminolysis such as glutaminase-1, Gln synthetase, glutamate dehydrogenase, and amino acid transaminase has shown promise in preclinical models. Future translation studies employing Gln delivery approaches and/or glutaminolysis inhibitors will determine the success of targeting Gln in cardiovascular disease.
The Relationship of Obesity, Nutritional Status and Muscle Wasting in Patients Assessed for Liver Transplantation
Authors: Vidot Helen , Kline Katharine , Cheng Robert , Finegan Liam , Lin Amelia , Kempler Elise , Strasser Simone I. , Bowen David Geoffrey , McCaughan Geoffrey William , Carey Sharon , Allman-Farinelli Margaret , Shackel Nicholas Adam
Published: September 2019

Introduction: Obesity co-exists with malnutrition and muscle atrophy in patients with cirrhosis. Muscle wasting is a feature of sarcopenia, a known determinant of patient outcomes. This is the first description of a relationship between obesity, subjective global assessment (SGA) of nutritional status and muscle wasting in patients with cirrhosis. Methods: The relationship between body mass index (BMI with obesity defined as ≥ 30 kg/m2), nutritional status (assessed by liver-specific subjective global assessment—SGA) and muscle wasting (assessed by corrected total cross-sectional psoas muscle area—cTPA) was analysed in patients with cirrhosis considered for liver transplantation between 1 January 2012 and 31 December 2014. Results: There were 205 patients, of whom 70% were males. The mean age was 52 ± 0.7 years and the Model for End-Stage Liver Disease (MELD) score was 16.8 ± 0.5. Overall, 31% of patients were obese and 56% of well-nourished (SGA A) individuals were obese. Muscle wasting was identified in 86% of all patients, irrespective of their nutritional status (A, B, C). All obese males classified as well-nourished (SGA A) were sarcopenic and 62% of obese females classified as SGA A were sarcopenic. Muscle wasting was worse in obese individuals (cTPA 230.9 mm2/m2 ± 12.9, p < 0.0001) and more likely to be associated with hepatic encephalopathy (p = 0.03). Univariate and multivariate analysis demonstrated testosterone deficiency was significantly associated with muscle wasting (p = 0.007) but not obesity (p = 0.8). Conclusion: Obesity combined with muscle wasting is common in patients with cirrhosis. Muscle wasting is common in well-nourished (SGA A) obese patients. Consequently, all patients assessed for liver transplantation should undergo additional screening for malnutrition and muscle wasting irrespective of BMI.
Selenium, Selenoproteins and Viral Infection
Authors: Guillin Olivia , Vindry Caroline , Ohlmann Théophile , Chavatte Laurent
Published: September 2019

Reactive oxygen species (ROS) are frequently produced during viral infections. Generation of these ROS can be both beneficial and detrimental for many cellular functions. When overwhelming the antioxidant defense system, the excess of ROS induces oxidative stress. Viral infections lead to diseases characterized by a broad spectrum of clinical symptoms, with oxidative stress being one of their hallmarks. In many cases, ROS can, in turn, enhance viral replication leading to an amplification loop. Another important parameter for viral replication and pathogenicity is the nutritional status of the host. Viral infection simultaneously increases the demand for micronutrients and causes their loss, which leads to a deficiency that can be compensated by micronutrient supplementation. Among the nutrients implicated in viral infection, selenium (Se) has an important role in antioxidant defense, redox signaling and redox homeostasis. Most of biological activities of selenium is performed through its incorporation as a rare amino acid selenocysteine in the essential family of selenoproteins. Selenium deficiency, which is the main regulator of selenoprotein expression, has been associated with the pathogenicity of several viruses. In addition, several selenoprotein members, including glutathione peroxidases (GPX), thioredoxin reductases (TXNRD) seemed important in different models of viral replication. Finally, the formal identification of viral selenoproteins in the genome of molluscum contagiosum and fowlpox viruses demonstrated the importance of selenoproteins in viral cycle.
Vitamin C and Neutrophil Function: Findings from Randomized Controlled Trials
Authors: Liugan Mikee , Carr Anitra C.
Published: September 2019

Vitamin C is known to support immune function and is accumulated by neutrophils to millimolar intracellular concentrations suggesting an important role for the vitamin in these cells. In this review, the effects of vitamin C, as a mono- or multi-supplement therapy, on neutrophil function were assessed by conducting a systematic review of randomized controlled trials (RCTs). Specifically, trials which assessed neutrophil migration (chemotaxis), phagocytosis, oxidative burst, enzyme activity, or cell death (apoptosis) as primary or secondary outcomes were assessed. A systematic literature search was conducted using the Cochrane Central Register of Controlled Trials, EMBASE, Embase Classic, Joanna Briggs Institute EBP, Ovid MEDLINE®, Ovid MEDLINE® In-Process & Other Non-Indexed Citations, Ovid Nursing Database, CINAHL and PubMed database, which identified 16 eligible RCTs. Quality appraisal of the included studies was carried out using the Cochrane Risk of Bias tool. Three of the studies assessed neutrophil chemotaxis in hospitalised patients or outpatients, two of which showed improved neutrophil function following intravenous vitamin C administration. Ten RCTs assessed neutrophil phagocytosis and/or oxidative burst activity; five were exercise studies, one in smokers, one in myocardial infarction patients and three in healthy volunteers. Two of the multi-supplement studies showed a difference between the intervention and control groups: increased oxidative burst activity in athletes post-exercise and decreased oxidant generation in myocardial infarction patients. Two studies assessed neutrophil enzyme activity; one showed deceased antioxidant enzyme activity in divers and the other showed increased antioxidant enzyme activity in athletes. One final study showed decreased neutrophil apoptosis in septic surgical patients following intravenous vitamin C administration. Overall, 44% of the RCTs assessed in this review showed effects of vitamin C supplementation on neutrophil functions. However, the studies were very heterogeneous, comprising different participant cohorts and different dosing regimens. There were also a number of limitations inherent in the design of many of these RCTs. Future RCTs should incorporate prescreening of potential participants for low vitamin C status or utilize cohorts known to have low vitamin status, such as hospitalized patients, and should also comprise appropriate vitamin C dosing for the cohort under investigation.
Effect of Bread Structure and In Vitro Oral Processing Methods in Bolus Disintegration and Glycemic Index
Authors: Aleixandre Andrea , Benavent-Gil Yaiza , Rosell Cristina M.
Published: September 2019

The growing interest in controlling the glycemic index of starchy-rich food has encouraged research about the role of the physical structure of food. The aim of this research was to understand the impact of the structure and the in vitro oral processing methods on bolus behavior and starch hydrolysis of wheat bread. Two different bread structures (loaf bread and bread roll) were obtained using different shaping methods. Starch hydrolysis during in vitro oro-gastro-intestinal digestion using the INFOGEST protocol was analyzed and oral processing was simulated by applying two different disintegration processes (basic homogenizer, crystal balls). The bread structure, and thus the shaping method during breadmaking, significantly affected the bolus particle size during all digestion stages. The different in vitro oral processing methods affected the bolus particle sizes after the oral phase in both breads, but they affected the particle size distribution after the gastric and intestinal phase only in the case of loaf bread. Aggregates were observed in the gastric phase, which were significantly reduced in the intestinal phase. When simulated oral processing with crystal balls led to bigger particle size distribution, bread rolls presented the highest in vitro starch hydrolysis. The type of in vitro oral processing allowed discrimination of the performance of the structures of the two breads during starch hydrolysis. Overall, crumb structure significantly affected texture properties, but also had a significant impact on particle size during digestion and starch digestibility.
Molecular Detection of Spotted-Fever Group Rickettsiae in Ticks Collected from Domestic and Wild Animals in Corsica, France
Authors: Cicculli Vincent , Maestrini Oscar , Casabianca Francois , Villechenaud Natacha , Charrel Remi , de Lamballerie Xavier , Falchi Alessandra
Published: September 2019

To obtain a better understanding of the current magnitude of tick-borne rickettsioses in Corsica, we used molecular methods to characterize the occurrence of Rickettsia spp. in ixodid ticks collected from domestic and wild animals. The presence of Rickettsia spp. was evaluated using real-time polymerase chain reaction targeting the gltA gene and by sequencing of gltA and ompA partial genes for species identification and phylogenetic analysis. Infection rates were calculated as the maximum-likelihood estimation (MLE) with 95% confidence intervals (CI). In total, 1117 ticks belonging to four genera (Rhipicephalus, Hyalomma, Ixodes, and Dermacentor) were collected from cattle, sheep, wild boars, and companion animals during July–August 2017 and July 2018–January 2019. Overall, Rickettsia DNA was detected in 208 of 349 pools of ticks (MLE = 25.6%, 95% CI: 22.6–28.8%). The molecular analysis revealed five different rickettsial species of the spotted-fever group (SFG). We highlighted the exclusive detection of Candidatus Ri. barbariae in R. bursa and of Ri. aeschlimanii in H. marginatum. Rickettsia slovaca was detected in D. marginatus collected from wild boars. This study provides the first evidence of the presence of Ri. monacensis in I. ricinus ticks isolated from a dog in Corsica. In conclusion, our data revealed wide dispersal of SFG Rickettsiae and their arthropod hosts in Corsica, highlighting the need for surveillance of the risk of infection for people living and/or working close to infected or infested animals.
Size Distribution of Colistin Delivery by Different Type Nebulizers and Concentrations During Mechanical Ventilation
Authors: Liu Ching-Yi , Ko Hsin-Kuo , Fink James , Wan Gwo-Hwa , Huang Chung-Chi , Chen Yu-Chun , Lin Hui-Ling
Published: September 2019

Although aerosol delivery through mechanical ventilators has been used to administer various medications, little is known of administration with colistin. This in vitro evaluation aimed to evaluate size distribution of colistin delivery by different types of nebulizers and concentrations during mechanical ventilation. Colistin methanesulfonate (colistin) for injection was dissolved in 6 mL of distilled water to produce a low concentration (L; 156 mg) and a high concentration (H; 312 mg). A dose volume of 6 mL was placed in a vibrating mesh nebulizer (VMN) and a jet nebulizer (JN). The inhaled mass (mean ± SD) of the VMN-L (53.80 ± 14.79 mg) was greater than both the JN-L (19.82 ± 3.34 mg, P = 0.001) and JN-H (31.72 ± 4.48 mg, P = 0.017). The nebulization time of the VMN-L (42.35 ± 2.30 min) was two times longer than the JN-L (21.12 ± 0.8 min) or JN-H (21.65 ± 0.42 min; P < 0.001). The mass median aerodynamic distal to the endotracheal tube was within a similar range at 2.03 to 2.26 μm (P = 0.434), independent of neb or formulation concentration. In conclusion, the VMN-L yields greater inhaled mass than the JN with either concentration. Therefore, a standard nominal dose of colistin results in a higher delivered dose during mechanical ventilation with a VMN compared with a JN and may be considered the preferred device. If JN must be used, multiple doses of low concentration colistin may compensate for poor delivery performance.
The Mechanical Properties of Poly (Urea-Formaldehyde) Incorporated with Nano-SiO2 by Molecular Dynamics Simulation
Authors: Zhang Yanfang , Wang Youyuan , Li Yudong , Zhang Zhanxi
Published: September 2019

Self-healing materials can promote the sustainable reuse of resources. Poly (urea-formaldehyde) (PUF) microcapsules can be incorporated into dielectric materials for self-healing. However, the mechanical properties of PUF microcapsules need to be improved due to insufficient hardness. In this paper, PUF models incorporated with nano-SiO2 of different filler concentrations (0, 2.6, 3.7, 5.3, 6.7, 7.9 wt.%) were designed. The density, the fractional free volume, and the mechanical properties of the PUF-SiO2 models were analyzed at an atomic level based on molecular dynamics simulation. The interfacial interaction model of PUF on the SiO2 surface was also constructed to further investigate the interaction mechanisms. The results showed that the incorporation of nano-SiO2 had a significant effect on the mechanical properties of PUF. Density increased, fractional free volume decreased, and mechanical properties of the PUF materials were gradually enhanced with the increase of nano-SiO2 concentration. This trend was also confirmed by experimental tests. By analyzing the internal mechanism of the PUF–SiO2 interfacial interaction, it was found that hydrogen bonds play a major role in the interaction between PUF and nano-SiO2. Moreover, hydrogen bonds can be formed between the polar atoms of the PUF chain and the hydroxyl groups (–OH) as well as O atoms on the surface of SiO2. Hydrogen bonds interactions are involved in adsorption of PUF chains on the SiO2 surface, reducing the distance between PUF chains and making the system denser, thus enhancing the mechanical properties of PUF materials.
Processing and Characterization of Polymer-Based Far-Infrared Composite Materials
Authors: Xiong Yabo , Zou Yang , Cai Shaoyong , Liu Huihui , Huang Shaoyun , Li Houbin
Published: September 2019

Polymer-based far-infrared radiation (FIR) composite materials are receiving increasing attention due to their significant influence on bioactivity. This study reports the processing of FIR composite films based on a polymer matrix and FIR radiation ceramic powders, as well as the characterization of the FIR composites. Field-emission scanning electron microscopy (SEM) and laser particle size analysis were employed to analyze the characteristic of the ceramic powders. The average size, dispersity, and specific surface area of the ceramic powders were 2602 nm, 0.97961, and 0.76 m2/g, respectively. The results show that the FIR ceramic powders used in the composite films had excellent far-infrared emissive performance. Moreover, by using differential scanning calorimetry (DSC) and thermogravimetric analysis (TG), it was indicated that the thermal performance and mechanical properties of the composite films were significantly influenced (p < 0.05) by the addition of the FIR ceramic powders. Specifically, the elongation at break decreased from 333 mm to 201 mm with the increase in FIR ceramic powders. Meanwhile, the contact angle and light transmittance were also changed by the addition of the FIR ceramic powders. Furthermore, the two different processing methods had great influence on the properties of the composite films. Moreover, the composite blown films with 1.5% FIR ceramic powders showed the highest far-infrared emissivity, which was 0.924.
An Experimental Study on Hydrodynamic Retention of Low and High Molecular Weight Sulfonated Polyacrylamide Polymer
Authors: Al-Hajri Sameer , Mahmood Syed , Abdulrahman Ahmed , Abdulelah Hesham , Akbari Saeed , Saraih Nabil
Published: September 2019

Polymers are often added with water as a viscosifier to improve oil recovery from hydrocarbon reservoirs. Polymer might be lost wholly or partially from the injected polymer solution by adsorption on the grain surfaces, mechanical entrapment in pores, and hydrodynamic retention in stagnant zones. Therefore, having a clear picture of polymer losses (and retention) is very important for designing a technically and economically successful polymer flood project. The polymer adsorption and mechanical entrapment are discussed more in depth in the literature, though the effect of hydrodynamic retention can be just as significant. This research investigates the effect of the hydrodynamic retention for low and high molecular weight (AN 113 VLM and AN 113 VHM) sulfonated polyacrylamide polymer. Two high permeability Bentheimer core plugs from outcrops were used to perform polymer corefloods. Polymer retention was first determined by injecting 1 cm3/min, followed by polymer core floods at 3, 5, and 8 cm3/min to determine the hydrodynamic retention (incremental retention). A higher molecular weight polymer (AN 113 VHM) showed higher polymer retention. In contrast, hydrodynamic retention for lower molecular weight (AN 113 VLM) was significantly higher than that of the higher molecular weight polymer. Other important observations were the reversibility of the hydrodynamic retention, no permanent permeability reduction, the shear thinning behavior in a rheometer, and shear thickening behavior in core floods.
Show Me the Money! Process Modeling in Pharma from the Investor’s Point of View
Authors: Varsakelis Christos , Dessoy Sandrine , von Stosch Moritz , Pysik Alexander
Published: September 2019

Process modeling in pharma is gradually gaining momentum in process development but budget restrictions are growing. We first examine whether and how current practices rationalize within a decision process framework with a fictitious investor facing a decision problem subject to incomplete information. We then develop an algorithmic procedure for investment evaluation on both monetary and diffusion-of-innovation fronts. Our methodology builds upon discounted cash flow analysis and Bayesian inference and utilizes the Rogers diffusion of innovation paradigm for computing lower expected returns. We also introduce a set of intangible metrics for quantifying the level of diffusion of process modeling within an organization.
An Integrated Approach, Orthodontic and Prosthetic, in a Case of Maxillary Lateral Incisors Agenesis
Authors: Portelli Marco , Militi Angela , Logiudice Antonino , Nucera Riccardo
Published: September 2019

Background: Among tooth anomalies, missing teeth is one of the most frequent, and it can be related to different therapeutical sets of problems. Often, an integrated approach that interests both orthodontists and prosthodontists is necessary, and in some cases also the periodontists. Methods: In this paper the authors report a clinical case of a 14-year-old patient, affected by maxillary bilateral incisors agenesis, molar bilateral II class and deep bite, treated in the Department of Orthodontics and Pedodontics of the University of Messina. The orthodontic treatment target was the distal movement of the maxillary molar, and the recovery of the space necessary for the prosthetic restoration of the missing lateral incisor. Maxillary molars distal movement was performed with a Distal Jet apparatus, skeletally supported by two miniscrews (Distal-Screw, American Orthodontics, Sheboygan, WI, USA). After molar relationship correction, a multi-bracket bimaxillary orthodontic appliance was bonded using Empower Brackets (American Orthodontics, Sheboygan, WI, USA). At the end of orthodontic treatment a Maryland bridge, bonded on the central incisors and cuspids, was used in order to maintain the space necessary for the insertion of dental implants in the region of 1.2 and 2.2. Results: A class II molar relationship was corrected, with an improvement of the deep bite, and the space necessary for implant insertion was recovered Conclusion: A skeletally supported Distal Jet was efficient for molar distalization, with the advantage of not having any loss of anchorage in the anterior part of the dental arch. This apparatus does not need patient compliance, have favorable aesthetics and also give the possibility to perform asymmetric activations.