bluuebunny/crossref_metadata_2025 huggingface dataset:
Unsupervised Semantic Segmentation Inpainting Network Using a Generative Adversarial Network with Preprocessing
Authors: Ahn Woo-Jin , Kim Dong-Won , Kang Tae-Koo , Pae Dong-Sung , Lim Myo-Taeg
Published: January 2023

The generative adversarial neural network has shown a novel result in the image generation area. However, applying it to a semantic segmentation inpainting task exhibits instability due to the different data distribution. To solve this problem, we propose an unsupervised semantic segmentation inpainting method using an adversarial deep neural network with a newly introduced preprocessing method and loss function. For stabilizing the adversarial training for semantic segmentation inpainting, we match the probability distribution of the segmentation maps with the developed preprocessing method. In addition, a new cross-entropy total variation loss for the probability map is introduced to improve the segmentation inpainting work by smoothing the segmentation map. The experimental results demonstrate the proposed algorithm’s effectiveness on both synthetic and real datasets.
Blockchain Secured Dynamic Machine Learning Pipeline for Manufacturing
Authors: Stodt Fatemeh , Stodt Jan , Reich Christoph
Published: January 2023

ML-based applications already play an important role in factories in areas such as visual quality inspection, process optimization, and maintenance prediction and will become even more important in the future. For ML to be used in an industrial setting in a safe and effective way, the different steps needed to use ML must be put together in an ML pipeline. The development of ML pipelines is usually conducted by several and changing external stakeholders because they are very complex constructs, and confidence in their work is not always clear. Thus, end-to-end trust in the ML pipeline is not granted automatically. This is because the components and processes in ML pipelines are not transparent. This can also cause problems with certification in areas where safety is very important, such as the medical field, where procedures and their results must be recorded in detail. In addition, there are security challenges, such as attacks on the model and the ML pipeline, that are difficult to detect. This paper provides an overview of ML security challenges that can arise in production environments and presents a framework on how to address data security and transparency in ML pipelines. The framework is presented using visual quality inspection as an example. The presented framework provides: (a) a tamper-proof data history, which achieves accountability and supports quality audits; (b) an increase in trust by protocol for the used ML pipeline, by rating the experts and entities involved in the ML pipeline and certifying legitimacy for participation; and (c) certification of the pipeline infrastructure, the ML model, data collection, and labelling. After describing the details of the new approach, the mitigation of the previously described security attacks will be demonstrated, and a conclusion will be drawn.
Experimental Study of Vertical Tail Model Flow Control Based on Oscillating Jet
Authors: Cao Xingyu , Dong Hao , Gu Yunsong , Cheng Keming , Zhang Fan
Published: January 2023

In this paper, wind tunnel experiments are conducted to study the control law and mechanism of oscillating jet flow control to improve the aerodynamic characteristics of the vertical tail when a civil aircraft encounters left side gust or significant crosswind during takeoff and landing. We measured the vertical tail scaling model’s aerodynamics, spatial flow field, and surface pressure when the Reynolds number was 2.12 × 105. The maximum momentum coefficient of the oscillating jet actuator reaches 0.332%. In addition, we studied the flow control effect of the three-dimensional vertical tail scaled model in different spanwise positions. The experimental results show that the oscillating jet at the rear edge of the stabilizer can significantly increase the lateral force of the vertical tail, and the increment of the lateral force can reach 36.5% under the worst condition of the negative side slip angle of the vertical tail. We can improve the lateral force coefficient of the vertical tail model by applying flow control alone at different spanwise locations. The wing root’s control effect and the vertical tail’s middle section are better than the wing tip’s. The oscillating jet can effectively restrain the flow separation on the rudder. In addition, the input of a high-energy jet “ejects” the mainstream, which increases the flow velocity at the side of the vertical tail actuator. It increases the circulation of the vertical tail. The oscillating jet flow control technology can effectively improve the vertical tail’s steering efficiency and increase the vertical tail’s lateral force, which is of great significance in improving the safety and economy of civil aircraft.
Development of Fiber Reinforced Sustainable Dredge Bricks
Authors: Nguyen Thi Thuy Minh , Rabbanifar Saeed , Luo Zhe , Huddleston Christopher , O’Connor Trey , Richard Adam , Michel Malik , Moon Ryan , Yao Chun-Wei , Jao Mien , Bernazzani Paul
Published: January 2023

To maintain adequate depth of commercial waterways, large quantities of earthen material are dredged and stored on undeveloped placement areas adjacent to the waterway. As dredge placement areas become overwhelmed, an environmental and financial sustainable solution for the reuse of dredged soil is prioritized. In this study, locally dredged material from the Sabine-Neches Waterway was used to explore the potential of dredged material in the production of compressed stabilized earth bricks (CSEBs) for small-scale structures in the region. CSEB mixture designs were developed containing fly ash (FA), Portland cement (PC), hydrated lime (HL), water (W), dredged material (DM), and natural and synthetic fibers. Optimized mixtures designs reached the recommended compressive strength of over 1200 psi. Results showed that that the addition of fibers reduced the compressive and flexural strength of the bricks, with a maximum compressive strength of 1394 psi with a corresponding flexural strength of 381 psi being obtained with fiberless dredge bricks. Multiple coating systems were also tested to increase the resistance of the bricks to weathering and erosion. Results showed that the use of coatings reduced water absorption and increased the bricks resistance to erosion, making them more adept in regions commonly subjected to flooding and heavy wind-driven rains.
Hg Content in EU and Non-EU Processed Meat and Fish Foods
Authors: Nava Vincenzo , Di Bella Giuseppa , Fazio Francesco , Potortì Angela Giorgia , Lo Turco Vincenzo , Licata Patrizia
Published: January 2023

Mercury is one of the most dangerous toxic elements. Fresh food is the primary source of Hg exposure for humans. However, since processed foods are also a fundamental part of the food system, continuous monitoring of this contaminant in processed products is necessary to safeguard consumer health. The purpose of this study was to determine the Hg content using a direct mercury analyzer (DMA-80) in different processed food products (beef, pork, poultry, shellfish, and fish species) of EU and non-EU origin purchased in supermarkets and ethnic food shops in Messina (Italy). The results obtained were variable and correlated with the different types of food matrix. Low mercury levels were obtained for beef, pork, and poultry products, ranging from <LOQ for most samples to 3.727 µg/Kg. Higher concentrations were, however, obtained for fish species (9.249–290.211 µg/Kg). The mercury content was below the maximum levels specified in Regulation No 1881/2006 as amended. Percentage of Tolerable Weekly Intake (TWI) values of total Hg were evaluated in accordance with the European Food Safety Authority (EFSA). The results showed that some of the pelagic species of the genus Thunnus thynnus exceeded the TWI values.
IGBT Overvoltage Protection Based on Dynamic Voltage Feedback and Active Clamping
Authors: Chen Mingfang , Xiong Zhichao , Zhang Yongxia , Zhu Enxiao , Zhao Yuying , Ma Zunbo
Published: January 2023

In view of the stability of the unmanned aerial vehicle (UAV) power system, this paper found that the voltage spike generated by IGBT mainly occurs when the IGBT is turned off in the study of the power system interior and IGBT drive and overvoltage protection, and an excessive voltage spike will lead to IGBT damage. To eliminate this serious threat to the safe operation of the circuit, an IGBT overvoltage protection circuit combining dynamic voltage feedback and active clamping is proposed. In this method, active clamping and dynamic voltage feedback circuits are operated alternately, and the drive circuit is controlled by the feedback of capacitor’s the dynamic voltage rise rate. The gate current output of the IGBT is directly compensated to control the signal delay of the gate, combined with improving the lifting effect of the active clamp circuit on the gate voltage and suppressing the peak of the turn-off voltage. According to the IGBT turn off process, combined with SABER simulation, this paper finally builds IGBT turn off experimental circuit to prove that the proposed method suppresses the size of the turn off voltage spike, further analyzes the effect of key circuit parameters on the suppression of the turn off voltage spike, thereby ensuring the safe operation of the IGBT, and improving the stability of the UAV power system.
Comparison of Topic Modelling Approaches in the Banking Context
Authors: Ogunleye Bayode , Maswera Tonderai , Hirsch Laurence , Gaudoin Jotham , Brunsdon Teresa
Published: January 2023

Topic modelling is a prominent task for automatic topic extraction in many applications such as sentiment analysis and recommendation systems. The approach is vital for service industries to monitor their customer discussions. The use of traditional approaches such as Latent Dirichlet Allocation (LDA) for topic discovery has shown great performances, however, they are not consistent in their results as these approaches suffer from data sparseness and inability to model the word order in a document. Thus, this study presents the use of Kernel Principal Component Analysis (KernelPCA) and K-means Clustering in the BERTopic architecture. We have prepared a new dataset using tweets from customers of Nigerian banks and we use this to compare the topic modelling approaches. Our findings showed KernelPCA and K-means in the BERTopic architecture-produced coherent topics with a coherence score of 0.8463.
Progressive Formation of Retrogressive Landslide and the Lateral Length of Instability
Authors: Zhu Xiongpeng , Xie Linglin , Tang Yi , Chen Yifan , Hu Huihua , Lu Guangyin , Chen Changfu , Lin Hang
Published: January 2023

Retrogressive landslide is caused by the lower rock mass sliding, so that the upper part loses support, is deformed, and starts to slide. In the process of highway construction, the incised slope often leads to retrogressive landslide, and the determination of the damage range of retrogressive landslide is of great significance for the control of the slope. Taking a highway retrogressive landslide in Hunan Province as the research object, the particle flow discrete element is used to numerically simulate the entire failure process of the slope. According to the complex geological conditions of the slope, the rock mass of each part of the slope model is divided, the displacement of key parts of the landslide is monitored, the whole failure process of the retrogressive landslide is simulated, and the lateral length of traction instability is calculated through the stability theory of the sliding pull-crack failure slope. The research shows that the incised slope is the root cause of the retrogressive landslide, and the rainfall is the direct cause. When the retrogressive landslide is treated in engineering practice, the lateral length of traction instability can be obtained according to the stability theory of the sliding pull-crack failure slope, to realize the accurate judgment of the traction failure range of the sliding body.
Highly Integrated Wideband Transmit/Receive Module for X-Band SAR Applications
Authors: de Paulis Francesco , Di Giuliomaria Daniele , Fina Antonio , Amici Marco , Mannocchi Giovanni , Di Carlofelice Alessandro , Fiaschetti Andrea , Tognolatti Piero , Orlandi Antonio
Published: January 2023

The roadmaps of satellite-based synthetic aperture radar (SAR) systems show a trend that requires a continuous improvement of the active antenna in terms of operating bandwidth, scanning angle capability, and swath width, thus leading to the demand for increased RF power and better power efficiency of the RF transmitter. Moreover, compact size and light weight are relevant objectives for making the overall SAR instrument appealing for future applications. The transmit/receive module (TRM) shown in this paper was developed while combining all these requirements in a cost-effective approach. A careful design of all relevant RF interconnects and passive devices was performed to ensure the largest output power from the last high-power amplifier of the transmitting chain, the lowest noise figure at the input of the receiving chain, and the calibration capability for appropriately tuning the TX and RX signal. The TRM was manufactured and experimentally tested to verify its performances. The measurement results show the superior performances of the proposed compact high-power large-bandwidth TRM. The achieved target objectives make the design of the proposed TRM readily applicable for the development of a compact, high-power, and highly integrated AESAs to be used for next-generation satellite constellations for Earth observation.
The Role and Relevance of Hearing Dogs from the Owner’s Perspective: An Explorative Study among Adults with Hearing Loss
Authors: Lalancette Audrey , Tremblay Marie-Alycia , Hotton Mathieu
Published: January 2023

This study aimed to explore perceptions and experiences about how owning a hearing dog can influence the functioning and the autonomy of people with hearing loss. Three adults participated in a semi-structured interview. The interviews were video recorded, transcribed, and coded. A procedure combining qualitative content analysis and interpretative phenomenological analysis was used. The study shows how specific aspects of hearing dogs are associated with increased autonomy and sense of security among owners. The attentive dog-owner pairing, the outstanding training and the companion role of the hearing dog are the main elements supporting the high satisfaction related by all the participants. In regard of the location context (Quebec, Canada), ongoing challenges for owners are reflected in the lack of visibility of this rehabilitation means and its poor recognition from the society, resulting in the constant burden to explain the dog’s work to others. For adults with hearing loss, the hearing dog is a relevant way of offering both the benefits of functional assistance and the psychosocial support of a pet. The association between owning a hearing dog and improved overall well-being suggests that this form of rehabilitation should be considered as a pertinent option by hearing health professionals.
Attitudes toward the Integration of Radiographers into the First-Line Interpretation of Imaging Using the Red Dot System
Authors: Oglat Ammar A. , Fohely Firas , Masalmeh Ali AL , Jbour Ismail AL , Jaradat Laith AL , Athamnah Sema I.
Published: January 2023

The red dot system uses expertise in the identification of anomalies to assist radiologists in distinguishing radiological abnormalities and managing them before the radiologist report is sent. This is a small step on the road to greater role development for radiographers. This practice has existed for more than 20 years in the UK. Today, it is only the UK seeking to legislate radiographer reports. The aim of this paper is to put focus on this issue, determine whether radiographer reports are necessary, and explore whether there are any benefits that can be highlighted to encourage health authorities worldwide to allow radiographers to write clinical reports. Additionally, this study was conducted to evaluate the role of radiographers (non-radiologists) in medical image interpretation, using 95 samples that were collected randomly and a representative sample of radiographers and radiologists of both genders. The SPSS program was used for the statistical analysis of the samples and to scientifically explain the results. We found that radiologists have no objections to the participation of radiographers in diagnosis assistance, interpretation, and clinical reporting through the red dot system. Therefore, there was support for the future implementation of such a system in health care.
Vascular Relaxation and Blood Pressure Lowering Effects of Prunus mume in Rats
Authors: Jo Cheolmin , Kim Bumjung , Lee Kyungjin , Choi Ho-Young
Published: January 2023

Prunus mume Siebold et Zuccarini is mainly consumed as processed fruits in beverages, vinegar, alcohol, or fruit syrup; studies have reported various functional effects. Many pharmacological and functional studies exist on fruit extracts or processed foods using fruits, however, efficacy studies on various parts of P. mume, including the bark, branches, flowers, and leaves, have not been sufficiently conducted. A previous study revealed that a 70% ethanol extract of P. mume branches induced vascular endothelium-dependent vasorelaxant effects in rat thoracic aortic rings. Therefore, we hypothesized that various parts (the fruits, flowers, leaves, and bark) might have vasorelaxant effects. We evaluated the effects of P. mume extracts on the vascular relaxation of isolated rat thoracic aorta and hypotensive effects in spontaneous hypertensive rats (SHR). A 70% ethanol extract of P. mume bark (PBaE) was the most effective, thus, we investigated its vasorelaxant mechanisms and hypotensive effects. PBaE lowered the blood pressure in SHR and induced the vascular endothelium-dependent relaxation of isolated rat aortic rings via the NO/sGC/cGMP and the PGI2 pathways in the vascular smooth muscle. Potassium channels, such as KCa, KATP, KV, and Kir, were partially associated with a PBaE-induced vasorelaxation. Therefore, PBaE might help prevent and treat hypertension.
Potential Utilization of Bacterial Consortium of Symbionts Marine Sponges in Removing Polyaromatic Hydrocarbons and Heavy Metals, Review
Authors: Marzuki Ismail , Rosmiati Rosmiati , Mustafa Akhmad , Sahabuddin Sahabuddin , Tarunamulia Tarunamulia , Susianingsih Endang , Hendrajat Erfan Andi , Sahrijanna Andi , Muslimin Muslimin , Ratnawati Erna , Kamariah Kamariah , Nisaa Khairun , Herlambang Susila , Gunawan Sri , Santi Idum Satia , Isnawan Bambang Heri , Kaseng Ernawati Syahruddin , Septiningsih Early , Asaf Ruzkiah , Athirah Admi , Basri Basri
Published: January 2023

Toxic materials in waste generally contain several components of the global trending pollutant category, especially PAHs and heavy metals. Bioremediation technology for waste management that utilizes microorganisms (bacteria) has not been fully capable of breaking down these toxic materials into simple and environmentally friendly chemical products. This review paper examines the potential application of a consortium of marine sponge symbionts with high performance and efficiency in removing PAHs and heavy metal contaminants. The method was carried out through a review of several related research articles by the author and published by other researchers. The results of the study conclude that the development of global trending pollutant (GTP) bioremediation technology could be carried out to increase the efficiency of remediation. Several types of marine sponge symbiont bacteria, hydrocarbonoclastic (R-1), metalloclastic (R-2), and metallo-hydro-carbonoclastic (R-3), have the potential to be applied to improve waste removal performance. A consortium of crystalline bacterial preparations is required to mobilize into GTP-exposed sites rapidly. Bacterial symbionts of marine sponges can be traced mainly to sea sponges, whose body surface is covered with mucus.
Chitinase-Assisted Bioconversion of Chitinous Waste for Development of Value-Added Chito-Oligosaccharides Products
Authors: Taokaew Siriporn , Kriangkrai Worawut
Published: January 2023

Chito-oligosaccharides (COSs) are the partially hydrolyzed products of chitin, which is abundant in the shells of crustaceans, the cuticles of insects, and the cell walls of fungi. These oligosaccharides have received immense interest in the last few decades due to their highly promising bioactivities, such as their anti-microbial, anti-tumor, and anti-inflammatory properties. Regarding environmental concerns, COSs are obtained by enzymatic hydrolysis by chitinase under milder conditions compared to the typical chemical degradation. This review provides updated information about research on new chitinase derived from various sources, including bacteria, fungi, plants, and animals, employed for the efficient production of COSs. The route to industrialization of these chitinases and COS products is also described.
Effect of a Selected Protective Culture of Lactilactobacillus sakei on the Evolution of Volatile Compounds and on the Final Sensorial Characteristics of Traditional Dry-Cured Fermented “Salchichón”
Authors: Martín Irene , García Carmen , Rodríguez Alicia , Córdoba Juan J.
Published: January 2023

Background: In this work, the effect of a selected starter culture of Lactilactobacillus sakei 205 on the evolution of volatile compounds throughout the ripening process and on the final sensorial characteristics of traditional dry-cured fermented “salchichón” was evaluated. Methods: “Salchichón” sausages were prepared, inoculated with L. sakei 205, and ripened for 90 days. Volatile compounds were analyzed throughout the ripening by GC-MS. In the final product, instrumental texture and color were determined. In addition, sensorial analysis was performed by a semi-trained panel. Results: The inoculation of L. sakei 205 does not influence the texture and color parameters of ripened “salchichón”. However, an increase in volatile compounds derived from amino acid catabolism and microbial esterification and a decrease in compounds derived from lipid oxidation, mainly hexanal, were observed throughout the ripening time as a consequence of L. sakei inoculation, which could have a positive effect on the flavor development of the dry-cured fermented “salchichón”. Conclusions: The use of selected strains of lactic acid bacteria (LAB) such as L. sakei 205 as a protective culture could be recommended to improve the quality of traditional “salchichón”.
Early Life Stress Detection Using Physiological Signals and Machine Learning Pipelines
Authors: Shahbazi Zeinab , Byun Yung-Cheol
Published: January 2023

Pregnancy and early childhood are two vulnerable times when immunological plasticity is at its peak and exposure to stress may substantially raise health risks. However, to separate the effects of adversity during vulnerable times of the lifetime from those across the entire lifespan, we require deeper phenotyping. Stress is one of the challenges which everyone can face with this issue. It is a type of feeling which contains mental pressure and comes from daily life matters. There are many research and investments regarding this problem to overcome or control this complication. Pregnancy is a susceptible period for the child and the mother taking stress can affect the child’s health after birth. The following matter can happen based on natural disasters, war, death or separation of parents, etc. Early Life Stress (ELS) has a connection with psychological development and metabolic and cardiovascular diseases. In the following research, the main focus is on Early Life Stress control during pregnancy of a healthy group of women that are at risk of future disease during their pregnancy. This study looked at the relationship between retrospective recollections of childhood or pregnancy hardship and inflammatory imbalance in a group of 53 low-income, ethnically diverse women who were seeking family-based trauma treatment after experiencing interpersonal violence. Machine learning Convolutional Neural Networks (CNNs) are applied for stress detection using short-term physiological signals in terms of non-linear and for a short term. The focus concepts are heart rate, and hand and foot galvanic skin response.
Dupilumab Leads to Clinical Improvements including the Acquisition of Tolerance to Causative Foods in Non-Eosinophilic Esophagitis Eosinophilic Gastrointestinal Disorders
Authors: Arakawa Naoya , Yagi Hisako , Shimizu Mariko , Shigeta Daisuke , Shimizu Akihiko , Nomura Shigeru , Takizawa Takumi , Yamada Yoshiyuki
Published: January 2023

A recent report showed that most pediatric cases of non-eosinophilic esophagitis (EoE) eosinophilic gastrointestinal disorders (EGIDs) (non-EoE EGIDs) are persistent and severe compared with those of EoE, thus requiring further effective therapeutic approaches. In this study, we present the first case based on a systematic search of non-EoE EGID for which tolerance to causative foods and histological and symptomatic improvements were achieved following dupilumab administration, after elimination diets and omalizumab and mepolizumab treatments. Driven by this case, we investigated the efficacies of biological treatments in non-EoE EGID cases based on the patient studied herein, and other patients identified in the conducted systematic review. Seven articles, including five different biologics, were reviewed. Both clinical efficacies and impact differences among the targeted molecules are demonstrated in this study. Our findings show that dupilumab may affect mechanisms that can suppress symptoms induced by offending foods that are different from those induced by other biologics as identified in the conducted systematic review. Additional studies are required to address the unmet needs of non-EoE EGID treatments.
Archaea as a Model System for Molecular Biology and Biotechnology
Authors: De Lise Federica , Iacono Roberta , Moracci Marco , Strazzulli Andrea , Cobucci-Ponzano Beatrice
Published: January 2023

Archaea represents the third domain of life, displaying a closer relationship with eukaryotes than bacteria. These microorganisms are valuable model systems for molecular biology and biotechnology. In fact, nowadays, methanogens, halophiles, thermophilic euryarchaeota, and crenarchaeota are the four groups of archaea for which genetic systems have been well established, making them suitable as model systems and allowing for the increasing study of archaeal genes’ functions. Furthermore, thermophiles are used to explore several aspects of archaeal biology, such as stress responses, DNA replication and repair, transcription, translation and its regulation mechanisms, CRISPR systems, and carbon and energy metabolism. Extremophilic archaea also represent a valuable source of new biomolecules for biological and biotechnological applications, and there is growing interest in the development of engineered strains. In this review, we report on some of the most important aspects of the use of archaea as a model system for genetic evolution, the development of genetic tools, and their application for the elucidation of the basal molecular mechanisms in this domain of life. Furthermore, an overview on the discovery of new enzymes of biotechnological interest from archaea thriving in extreme environments is reported.
Fetal Growth Restriction Impairs Lung Function and Neurodevelopment in an Early Preterm Rabbit Model
Authors: Valenzuela Ignacio , Zapletalova Katerina , Greyling Marnel , Regin Yannick , Gie Andre , Basurto David , Deprest Jan , van der Merwe Johannes
Published: January 2023

We previously reported the multi-system sequelae of fetal growth restriction, induced by placental underperfusion, in near-term born rabbits, in the immediate neonatal period and up to pre-adolescence. Herein, we describe the pulmonary and neurodevelopmental consequences of FGR in rabbits born preterm. We hypothesize that FGR has an additional detrimental effect on prematurity in both pulmonary function and neurodevelopment. FGR was induced at gestational day (GD) 25 by placental underperfusion, accomplished by partial uteroplacental vessel ligation in one uterine horn. Rabbits were delivered by cesarean section at GD 29, and placentas were harvested for histology. Neonates underwent neurobehavioral or pulmonary functional assessment at postnatal day 1, followed by brain or lung harvesting, respectively. The neurodevelopmental assessment included neurobehavioral testing and multiregional quantification of cell density and apoptosis in the brain. Lung assessment included functional testing, alveolar morphometry, and airway histology. FGR was associated with higher perinatal mortality, lower birth and placental weight, and a similar brain-to-body weight ratio compared to controls. Placental underperfusion decreased labyrinth and junction zone volumes in FGR placentas. FGR impaired pulmonary function, depicted by higher parenchymal resistance, damping, and elastance. Alveolar morphometry and airway smooth muscle content were comparable between groups. Neurobehavioral tests showed motoric and sensorial impairment in FGR rabbits. In FGR brains, cell density was globally reduced, with higher apoptosis in selected areas. In conclusion, in preterm-born rabbits, placental underperfusion leads to higher mortality, FGR, and impaired lung and brain development in early assessment. This study complements previous findings of placental, pulmonary, and neurodevelopmental impairment in near-term born rabbits in this model.
Extracorporeal Carbon Dioxide Removal: From Pathophysiology to Clinical Applications; Focus on Combined Continuous Renal Replacement Therapy
Authors: Cappadona Francesca , Costa Elisa , Mallia Laura , Sangregorio Filippo , Nescis Lorenzo , Zanetti Valentina , Russo Elisa , Bianzina Stefania , Viazzi Francesca , Esposito Pasquale
Published: January 2023

Lung-protective ventilation (LPV) with low tidal volumes can significantly increase the survival of patients with acute respiratory distress syndrome (ARDS) by limiting ventilator-induced lung injuries. However, one of the main concerns regarding the use of LPV is the risk of developing hypercapnia and respiratory acidosis, which may limit the clinical application of this strategy. This is the reason why different extracorporeal CO2 removal (ECCO2R) techniques and devices have been developed. They include low-flow or high-flow systems that may be performed with dedicated platforms or, alternatively, combined with continuous renal replacement therapy (CRRT). ECCO2R has demonstrated effectiveness in controlling PaCO2 levels, thus allowing LPV in patients with ARDS from different causes, including those affected by Coronavirus disease 2019 (COVID-19). Similarly, the suitability and safety of combined ECCO2R and CRRT (ECCO2R–CRRT), which provides CO2 removal and kidney support simultaneously, have been reported in both retrospective and prospective studies. However, due to the complexity of ARDS patients and the limitations of current evidence, the actual impact of ECCO2R on patient outcome still remains to be defined. In this review, we discuss the main principles of ECCO2R and its clinical application in ARDS patients, in particular looking at clinical experiences of combined ECCO2R–CRRT treatments.
Design of a Magnetic Soft Inchworm Millirobot Based on Pre-Strained Elastomer with Micropillars
Authors: Wei Yuzhang , Wu Zehao , Dai Ziyi , Zhou Bingpu , Xu Qingsong
Published: January 2023

Rather than using longitudinal “muscle” as in biological inchworm, the existing magnetic active elastomer (MAE)-based inchworm robots utilize magnetic torque to pull and push the soft body, which hinders its locomotion mobility. In this paper, a new pre-strained MAE inchworm millirobot with micropillars is proposed. The pre-strained elastomer serves as a pre-load muscle to contract the soft body, and the micropillars act as tiny feet to anchor the body during the locomotion. The proposed magnetic inchworm robot features a simple fabrication process that does not require special magnetization equipment. For the first time, the pre-load muscle is introduced in the design of magnetic inchworm robots, making it more like a real inchworm in terms of locomotion mechanism. The locomotion principle and parametric design for the desired locomotion performance have been investigated. Experimental results show that the fabricated magnetic inchworm robot (size: 10 mm × 5 mm, micropillars length: 200 µm, and mass: 262 g) can locomote on a smooth acrylic surface (roughness of 0.3 µm) at the speed of 0.125 body lengths per second, which is comparable with the existing magnetic inchworm robots. Moreover, the locomotion capabilities of the inchworm robot on wet surfaces and inclined planes have been verified via experimental studies.
Rapid Characterization of Black Tea Taste Quality Using Miniature NIR Spectroscopy and Electronic Tongue Sensors
Authors: Ren Guangxin , Zhang Xusheng , Wu Rui , Yin Lingling , Hu Wenyan , Zhang Zhengzhu
Published: January 2023

The taste of tea is one of the key indicators in the evaluation of its quality and is a key factor in its grading and market pricing. To objectively and digitally evaluate the taste quality of tea leaves, miniature near-infrared (NIR) spectroscopy and electronic tongue (ET) sensors are considered effective sensor signals for the characterization of the taste quality of tea leaves. This study used micro-NIR spectroscopy and ET sensors in combination with data fusion strategies and chemometric tools for the taste quality assessment and prediction of multiple grades of black tea. Using NIR features and ET sensor signals as fused information, the data optimization based on grey wolf optimization, ant colony optimization (ACO), particle swarm optimization, and non-dominated sorting genetic algorithm II were employed as modeling features, combined with support vector machine (SVM), extreme learning machine and K-nearest neighbor algorithm to build the classification models. The results obtained showed that the ACO−SVM model had the highest classification accuracy with a discriminant rate of 93.56%. The overall results reveal that it is feasible to qualitatively distinguish black tea grades and categories by NIR spectroscopy and ET techniques.
Chitosan-Based Hydrogels for Bioelectronic Sensing: Recent Advances and Applications in Biomedicine and Food Safety
Authors: Wu Si , Wu Shijing , Zhang Xinyue , Feng Tao , Wu Long
Published: January 2023

Due to the lack of efficient bioelectronic interfaces, the communication between biology and electronics has become a great challenge, especially in constructing bioelectronic sensing. As natural polysaccharide biomaterials, chitosan-based hydrogels exhibit the advantages of flexibility, biocompatibility, mechanical tunability, and stimuli sensitivity, and could serve as an excellent interface for bioelectronic sensors. Based on the fabrication approaches, interaction mechanisms, and bioelectronic communication modalities, this review divided chitosan-based hydrogels into four types, including electrode-based hydrogels, conductive materials conjugated hydrogels, ionically conductive hydrogels, and redox-based hydrogels. To introduce the enhanced performance of bioelectronic sensors, as a complementary alternative, the incorporation of nanoparticles and redox species in chitosan-based hydrogels was discussed. In addition, the multifunctional properties of chitosan-based composite hydrogels enable their applications in biomedicine (e.g., smart skin patches, wood healing, disease diagnosis) and food safety (e.g., electrochemical sensing, smart sensing, artificial bioelectronic tongue, fluorescence sensors, surface-enhanced Raman scattering). We believe that this review will shed light on the future development of chitosan-based biosensing hydrogels for micro-implantable devices and human–machine interactions, as well as potential applications in medicine, food, agriculture, and other fields.
Metal–Organic Framework Fluorescence Sensors for Rapid and Accurate Detection of Melamine in Milk Powder
Authors: Alizadeh Sani Mahmood , Jahed-Khaniki Gholamreza , Ehsani Ali , Shariatifar Nabi , Dehghani Mohammad Hadi , Hashemi Mohammad , Hosseini Hedayat , Abdollahi Mohammad , Hassani Shokoufeh , Bayrami Zahra , McClements David Julian
Published: January 2023

In this research, a simple, label-free, and ultra-sensitive fluorescent platform based on a metal–organic framework (MOF) has been developed to detect melamine in milk powder. This fluorescence sensor was fabricated from sensitized terbium (Tb)@NH2-MIL-253 (Al) MOF using a hydrothermal method that involved combining the green emission of Tb (λem = 545 nm) with the blue emission of NH2-MIL-253(Al) MOF (λem = 430 nm) under a single excitation wavelength (λex = 335 nm). The fluorescence sensor was then used under optimized conditions (pH = 9.0; sensor concentration = 30 mg/L; response time = 30 s) to quantify melamine in milk powder. The accuracy, sensitivity, and reproducibility of this sensor were established compared to the high-performance liquid chromatography (HPLC) method. The linear range and lower limit of detection (LLOD, computed with 3σ/S) of the sensor were between 40–396.45 nM (equal to 25 µg/kg–0.25 mg/kg) and 40 nM (equal to 25 µg/kg), respectively, which is much less than the maximum residual level (MRL) for the detection of melamine in infant formula (1 mg/kg) and other foods/feeds (2.5 mg/kg). Additionally, the results had good agreement with the HPLC outcomes, suggesting that the NH2-MIL-253(Al) MOF sensing probe has great precision and repeatability. To conclude, the new fluorescence sensor developed in this study can accurately and sensitively detect melamine in food samples, which may be useful for screening for adulteration of milk powders and other foods.
Influence of Sex and Muscarinic Activity on Memory Retrieval in Mouse Model of Traumatic Brain Injury
Authors: Rashid Habiba , Ahmed Touqeer
Published: January 2023

Traumatic brain injury (TBI) is a serious global risk factor leading to the onset of cognitive impairment and neurodegenerative diseases. Cognitive and memory impairment following a TBI is associated with the dysregulation of cholinergic neurotransmission in the brains of subjects. The extent of memory impairment following a TBI is linked with the sex of the subject. This study aimed to identify the sex-dimorphic role of muscarinic cholinergic modulation in neurological functioning and episodic memory retrieval in a mouse model of TBI. Balb/c mice were divided into four groups of males and four groups of females (i.e., Sham, TBI, TBI + Scopolamine 1 mg/kg, and TBI + Donepezil 1 mg/kg). After training with the Morris water maze test and fear conditioning, all groups were subjected to brain injury (7.84 × 10−5 J impact force) except for the Sham mice. Following brain injury, scopolamine or donepezil was administered to the respective groups for 5 days. Acute scopolamine immediately after brain trauma showed a neuroprotective effect in the males only, while subchronic donepezil significantly impaired neurological functioning in both sexes. Subchronic scopolamine and donepezil treatment reversed the TBI-induced retrograde amnesia for spatial memory in male mice. Contextual fear memory retrieval was not affected by the TBI and treatments in both sexes. Thus, we concluded that the sex-dimorphic response of the muscarinic receptors in TBI-induced memory impairment depends on the type of memory. This study highlights the potential for therapeutic modalities in TBI subjects.
A New Construct in Career Research: Career Crafting
Authors: Ge Xiaolin , Gao Lei , Yu Haibo
Published: January 2023

Career crafting is a new concept in the field of career research in recent years. However, the research on career crafting is still in its infancy, and there are few systematic and integrated studies. In this study, we have collected the existing research and extracted the 12 most related articles from 10 databases (Web of Science, Google Scholar, ProQuest, and EBSCO Host, etc.) by the end of 2022 to discuss the concepts of discrimination, theoretical basis, research methods, and measurement tools and variables of career crafting. As a reference for the follow-up in-depth study, future research should progress forward, such as by deepening and expanding the theoretical basis, testing and developing mature scales, building multilevel influencing factors and testing their interaction, and furthering the research on the mechanism of multi-field effects.
Are Thalamic Intrinsic Lesions Operable? No-Man’s Land Revisited by the Analysis of a Large Retrospective, Mono-Institutional, Cohort
Authors: Ferroli Paolo , Restelli Francesco , Bertolini Giacomo , Monti Emanuele , Falco Jacopo , Bonomo Giulio , Tramacere Irene , Pollo Bianca , Calatozzolo Chiara , Patanè Monica , Schiavolin Silvia , Broggi Morgan , Acerbi Francesco , Erbetta Alessandra , Esposito Silvia , Mazzapicchi Elio , La Corte Emanuele , Vetrano Ignazio Gaspare , Broggi Giovanni , Schiariti Marco
Published: January 2023

Thalamic gliomas represent a heterogeneous subset of deep-seated lesions for which surgical removal is advocated, although clear prognostic factors linked to advantages in performance status or overall survival are still lacking. We reviewed our Institutional Cancer Registry, identifying patients who underwent surgery for thalamic gliomas between 2006 and 2020. Associations between possible prognostic factors such as tumor volume, grade, the extent of resection and performance status (PS), and overall survival (OS) were evaluated using univariate and multivariate survival analyses. We found 56 patients: 31 underwent surgery, and 25 underwent biopsy. Compared to biopsy, surgery resulted positively associated with an increase in the OS (hazard ratio, HR, at multivariate analysis 0.30, 95% confidence interval, CI, 0.12–0.75). Considering the extent of resection (EOR), obtaining GTR/STR appeared to offer an OS advantage in high-grade gliomas (HGG) patients submitted to surgical resection if compared to biopsy, although we did not find statistical significance at multivariate analysis (HR 0.53, 95% CI 0.17–1.59). Patients with a stable 3-month KPS after surgery demonstrated to have a better prognosis in terms of OS if compared to biopsy (multivariate HR 0.17, 95% CI, 0.05–0.59). Age and histological grades were found to be prognostic factors for this condition (p = 0.04 and p = 0.004, respectively, chi-square test). Considering the entire cohort, p53 positivity (univariate HR 2.21, 95% CI 1.01–4.82) and ATRX positivity (univariate HR 2.69, 95% CI 0.92–7.83) resulted associated with a worse prognosis in terms of OS. In this work, we demonstrated that surgery aimed at tumor resection might offer a stronger survival advantage when a stable 3-month KPS after surgery is achieved.
STARD3: A New Biomarker in HER2-Positive Breast Cancer
Authors: Lodi Massimo , Voilquin Laetitia , Alpy Fabien , Molière Sébastien , Reix Nathalie , Mathelin Carole , Chenard Marie-Pierrette , Tomasetto Catherine-Laure
Published: January 2023

Pathological complete response (pCR) after neoadjuvant systemic treatment (NST) is an important prognostic factor in HER2-positive breast cancer. The majority of HER2-positive breast cancers are amplified at the HER2 gene locus, several genes are co-amplified with HER2, and a subset of them are co-expressed. The STARD3 gene belongs to the HER2 amplicon, and its role as a predictive marker was never addressed. The objective of this study was to investigate the predictive value of STARD3 protein expression on NST pathological response in HER2-positive breast cancer. In addition, we studied the prognostic value of this marker. Methods. We conducted a retrospective study between 2007 and 2020 on 112 patients with non-metastatic HER2-positive breast cancer treated by NST and then by surgery. We developed an immunohistochemistry assay for STARD3 expression and subcellular localization and determined a score for STARD3-positivity. As STARD3 is an endosomal protein, its expression was considered positive if the intracellular signal pattern was granular. Results: In this series, pCR was achieved in half of the patients. STARD3 was positive in 86.6% of cases and was significantly associated with pCR in univariate analysis (p = 0.013) and after adjustment on other known pathological parameters (p = 0.044). Performances on pCR prediction showed high sensitivity (96%) and negative predictive value (87%), while specificity was 23% and positive predictive value was 56%. Overall, specific, relapse-free, and distant metastasis-free survivals were similar among STARD3 positive and negative groups, independently of other prognosis factors. Conclusion: NST is an opportunity for HER2-positive cancers. In this series of over a hundred HER2-positive and non-metastatic patients, a STARD3-negative score was associated with the absence of pathological complete response. This study suggests that determining STARD3 overexpression status on initial biopsies of HER2-positive tumors is an added value for the management of a subset of patients with high probability of no pathological response.
Radiomic Analysis Based on Magnetic Resonance Imaging for Predicting PD-L2 Expression in Hepatocellular Carcinoma
Authors: Tao Yun-Yun , Shi Yue , Gong Xue-Qin , Li Li , Li Zu-Mao , Yang Lin , Zhang Xiao-Ming
Published: January 2023

Hepatocellular carcinoma (HCC) is the sixth most common malignant tumour and the third leading cause of cancer death in the world. The emerging field of radiomics involves extracting many clinical image features that cannot be recognized by the human eye to provide information for precise treatment decision making. Radiomics has shown its importance in HCC identification, histological grading, microvascular invasion (MVI) status, treatment response, and prognosis, but there is no report on the preoperative prediction of programmed death ligand-2 (PD-L2) expression in HCC. The purpose of this study was to investigate the value of MRI radiomic features for the non-invasive prediction of immunotherapy target PD-L2 expression in hepatocellular carcinoma (HCC). A total of 108 patients with HCC confirmed by pathology were retrospectively analysed. Immunohistochemical analysis was used to evaluate the expression level of PD-L2. 3D-Slicer software was used to manually delineate volumes of interest (VOIs) and extract radiomic features on preoperative T2-weighted, arterial-phase, and portal venous-phase MR images. Least absolute shrinkage and selection operator (LASSO) was performed to find the best radiomic features. Multivariable logistic regression models were constructed and validated using fivefold cross-validation. The area under the receiver characteristic curve (AUC) was used to evaluate the predictive performance of each model. The results show that among the 108 cases of HCC, 50 cases had high PD-L2 expression, and 58 cases had low PD-L2 expression. Radiomic features correlated with PD-L2 expression. The T2-weighted, arterial-phase, and portal venous-phase and combined MRI radiomics models showed AUCs of 0.789 (95% CI: 0.702–0.875), 0.727 (95% CI: 0.632–0.823), 0.770 (95% CI: 0.682–0.875), and 0.871 (95% CI: 0.803–0.939), respectively. The combined model showed the best performance. The results of this study suggest that prediction based on the radiomic characteristics of MRI could noninvasively predict the expression of PD-L2 in HCC before surgery and provide a reference for the selection of immune checkpoint blockade therapy.