bluuebunny/crossref_metadata_2025 huggingface dataset:
Potato Yield, Net Revenue and Specific Gravity Responses to Nitrogen Fertilizer under Different Canadian Agroecozones
Authors: Nyiraneza Judith , Cambouris Athyna N. , Nelson Alison , Khakbazan Mohammad , Mesbah Morteza , Perron Isabelle , Ziadi Noura , Lafond Jean
Published: July 2021

Applying higher nitrogen (N) rates than required for optimum potato (Solanum tuberosum L.) growth leads to economic and environmental losses. The extent to which the N rate associated with maximum potato yields differs from that maximizing net revenue (NR) or potato specific gravity is not fully understood. The objectives of this three-year study (2013–2015) conducted at five sites in three Canadian provinces (MB-1; MB-2; QC-1; QC-2; PEI) (15 site-years) were to: (i) assess potato marketable yield, NR, and specific gravity responses to increasing N application; (ii) calculate the N rate maximizing marketable (Nmax) yield and NR using different statistical models. The year, N fertilizer, and their interaction were significant on marketable yield and NR except at the MB-1 site where no significant effect of N was observed. No significant yield increases were observed at a N rate above 60 kg N ha−1 at four site-years and above 120 kg N ha−1 at five site-years, implying that the current recommended N rate could be reduced. All models fitted the marketable and NR data equally based on R2, mean bias error or root mean square error and resulted in comparable predicted yield and NR values. However, Nmax values were different depending on the model with higher values being predicted by the quadratic- (161.4 to 191.9 kg N ha−1) and the quadratic plateau models (60 to 191.9 kg N ha−1), while lower Nmax values were obtained with linear plateau- (60.6 to 129.8 kg N ha−1) and Mitscherlich–Baule plateau models (60.9 to 130. 9 kg N ha−1). Nitrogen rate maximizing NR was on average 4% lower than the N rate maximizing marketable yields, except at one site where it was higher by 26 kg N ha−1 when the quadratic plus plateau model was used. Specific gravity tended to decrease with the N rate. Our study confirms trade-offs between the N rate maximizing yields or NR with that maximizing specific gravity. Nitrogen rate maximizing marketable yield and NR varies depending on the selected model.
Application of Biochar Produced from Crop Residues on Trace Elements Contaminated Soils: Effects on Soil Properties, Enzymatic Activities and Brassica rapa Growth
Authors: Campos Paloma , Knicker Heike , López Rafael , De la Rosa José María
Published: July 2021

Soil pollution by trace elements is a huge problem around the globe. In addition, heavy metal immobilization and primary productivity are two soil ecosystem services of contemporary importance to society. The goal of this study was to evaluate the effects of using olive pit and rice husk biochars as soil amendments for the immobilization of trace elements and on plant development growing in heavy metals-polluted soils under greenhouse conditions. The application of high doses (5% and 10%) of biochar significantly increased pH, water holding capacity and total C content of the soils. Dehydrogenase activity in the moderately acidic soil was greater than in the acidic soil due to the high concentration of metals and high acidity of the latter. The application of biochar reduced the β–glucosidase activity. Furthermore, the concentrations of CaCl2-extractable heavy metals significantly decreased in biochar amended pots, indicating metal immobilization, which was consistent with the increase in soil pH. Distribution of trace elements in the different fractions was modified after 65 days of incubation, independently of the treatment. The Cu and Zn contents in the oxidizable fraction were reduced with incubation, whereas Cd and Zn in the residual fraction increased. The reduction of bioavailable concentrations and increments in the residual or more stable fractions indicated less risk for the organisms in the environment. All biochars addition significantly increased the root-to-shoot ratio compared to the control soil. Particularly, 10% of amendment increased this ratio in the greatest extent. The application of 10% w/w of rice husk biochar produced at 500 °C was the most effective in restoring soil functionality and reducing the availability of heavy metals in the polluted soils.
Cultivar and Phenological Stage Effects on the Success of In Vitro Meristem Culture and GLRaV-3 Elimination of Croatian Autochthonous Grapevine Cultivars
Authors: Marković Zvjezdana , Zrilić Anđela , Šikuten Iva , Štambuk Petra , Tomaz Ivana , Vončina Darko , Maletić Edi , Kontić Jasminka Karoglan , Preiner Darko
Published: July 2021

The population of Croatian autochthonous cultivars has a high degree of infection with economically important viruses, so it is necessary to carry out the elimination of the viruses in some cultivars to obtain healthy planting material. In this research, we tested in vitro meristem culture establishment on 18 autochthonous cultivars with different viral infections and the possibility of GLRaV-3 elimination through in vitro meristem culture. Plant material was sampled in a vineyard in two phenological stages, 10 days before flowering and 10 days after flowering of the grapevine. Apical meristem explants (1 mm) were placed into the MS culture medium supplemented with 0.5 mg/L benzyl adenine (BA) and 0.05 mg/L indol-3-acetic acid (IAA), and their survival, regeneration, and rooting were monitored. The results showed that the cultivar and the growth phase have a significant impact on the success of in vitro culture. In all cultivars studied higher success of in vitro culture establishment parameters (survival, regeneration, and rooting) was obtained in the case of explants sampled after flowering, with the exception of one cultivar for explants survival. Contrary to expectations, genotypes infected with three viruses (GLRaV-1, GLRaV-3, and GFLV) showed better results than genotypes infected with one or two viruses. The results showed successful in vitro establishment of Croatian autochthonous cultivar and GRLaV-3 elimination in one cultivar. However, due to the significant effect of cultivar, for routine application of this in vitro protocol on more than 100 autochthonous cultivars in need of sanitation, further studies should be conducted.
Intramuscular Fatty Acids in Meat Could Predict Enteric Methane Production by Fattening Lambs
Authors: Requena Domenech Francisco , Gómez-Cortés Pilar , Martínez-Miró Silvia , de la Fuente Miguel Ángel , Hernández Fuensanta , Martínez Marín Andrés Luis
Published: July 2021

Methane (CH4) emissions pose a serious problem for the environmental sustainability of ruminant production. The aim of the present study was to explore the usefulness of the intramuscular fatty acid (FA) profile to estimate CH4 production of lambs fattened under intensive feeding systems. A statistical regression analysis of intramuscular FA derived from ruminal metabolism was carried out to assess the best predictive model of CH4 production (g/d) in lambs fed with different diets. CH4 was calculated with three distinct equations based on organic matter digestibility (OMD) at maintenance feeding levels. The OMD of the experimental diets was determined in an in vivo digestibility trial by means of the indicator method. Regression models were obtained by stepwise regression analysis. The three optimized models showed high adjusted coefficients of determination (R2adj = 0.74–0.93) and concordance correlation coefficients (CCC = 0.89–0.98), as well as small root mean square prediction errors (RMSPE = 0.29–0.40 g/d). The best single predictor was vaccenic acid (trans-11 C18:1), a bioactive FA that is formed in the rumen to a different extent depending on dietary composition. Based on our data and further published lamb research, we propose a novel regression model for CH4 production with excellent outcomes: CH4 (g/d) = −1.98 (±1.284)–0.87 (±0.231) × trans-11 C18:1 + 0.79 (±0.045) × BW (R2adj = 0.97; RMSPE = 0.76 g/d; CCC = 0.98). In conclusion, these results indicate that specific intramuscular FA and average BW during fattening could be useful to predict CH4 production of lambs fed high concentrate diets.
Effect of Lipopolysaccharides (LPS) and Lipoteichoic Acid (LTA) on the Inflammatory Response in Rumen Epithelial Cells (REC) and the Impact of LPS on Claw Explants
Authors: Reisinger Nicole , Wendner Dominik , Schauerhuber Nora , Mayer Elisabeth
Published: July 2021

Endotoxins play a crucial role in ruminant health due to their deleterious effects on animal health. The study aimed to evaluate whether LPS and LTA can induce an inflammatory response in rumen epithelial cells. For this purpose, epithelial cells isolated from rumen tissue (REC) were stimulated with LPS and LTA for 1, 2, 4, and 24 h. Thereafter, the expression of selected genes of the LPS and LTA pathway and inflammatory response were evaluated. Furthermore, it was assessed whether LPS affects inflammatory response and structural integrity of claw explants. Therefore, claw explants were incubated with LPS for 4 h to assess the expression of selected genes and for 24 h to evaluate tissue integrity via separation force. LPS strongly affected the expression of genes related to inflammation (NFkB, TNF-α, IL1B, IL6, CXCL8, MMP9) in REC. LTA induced a delayed and weaker inflammatory response than LPS. In claw explants, LPS affected tissue integrity, as there was a concentration-dependent decrease of separation force. Incubation time had a strong effect on inflammatory genes in claw explants. Our data suggest that endotoxins can induce a local inflammatory response in the rumen epithelium. Furthermore, translocation of LPS might negatively impact claw health.
Is Animal Welfare an Internationally Understood Concept in the Zoo World? Thematic Analysis of Two Regional Groups of Zoo Staff
Authors: Bacon Heather , Vigors Belinda , Shaw Darren J. , Waran Natalie , Dwyer Cathy M. , Bell Catriona
Published: July 2021

Universal frameworks for zoo animal welfare have been suggested. However, there is little evidence of a cross-cultural understanding of zoo animal welfare. This paper reports themes emerging from a qualitative study of international (European and Chinese) zoo professionals on zoo animal welfare issues. Structured telephone interviews were conducted with eight Chinese and eight European zoo staff, covering aspects of zoological animal welfare, conservation and zoological husbandry practices. These qualitative data were thematically analysed, and key themes generated. This paper describes three themes relating to ‘What is animal welfare’ ‘Animal welfare frameworks’ and ‘The human element in animal welfare’. This analysis indicates that the concept of animal welfare has cultural equivalence across Europe and between Europe and China, and that zoo staff are familiar with welfare frameworks. In China, a need for senior leadership and motivating staff to improve animal welfare emerged.
The Association between Palmer Drought Severity Index Data and Tuberculosis-like Lesions Occurrence in Mediterranean Hunted Wild Boars
Authors: Abrantes Ana Carolina , Serejo João , Vieira-Pinto Madalena
Published: July 2021

In the Iberian Peninsula, the prevalence of tuberculosis differs for each region and for different wild disease hosts and the region affected by a Mediterranean climate will be the most affected. The Mediterranean Iberic regions have a favourable ecosystem for the development of Mycobacterium tuberculosis complex agents, where habitat, population dynamics, and climate (especially drought) are important factors affecting the high prevalence of tuberculosis in the wild boar population. Our objective was to study the association between the Palmer Drought Severity Index (PDSI) and the occurrence of tuberculosis-like lesions (TBL) in wild boar during nine hunting seasons (2008/09 to 2016/17) in Idanha-a-Nova County. To this end, statistical analysis revealed a significant association (p < 0.05) between the occurrence of TBL in wild boar in Idanha-a-Nova County and the analysed risk factor—previous season with periods of drought—which indicated that, when one season experiences some periods of drought, the probability of TBL occurrence in wild boars was 1.2 (OR = 1.2) times higher in the next hunting season than when there were no periods of drought. Therefore, our study contributes to the discovery of a positive effect of periods of drought on the transmission of tuberculosis in Iberian wildlife.
Effects of Allogeneic Mesenchymal Stem Cell Transplantation in Dogs with Inflammatory Bowel Disease Treated with and without Corticosteroids
Authors: Cristóbal José Ignacio , Duque Francisco Javier , Usón-Casaús Jesús María , Ruiz Patricia , Nieto Esther López , Pérez-Merino Eva María
Published: July 2021

Mesenchymal stem cells have proven to be a promising alternative to conventional steroids to treat canine inflammatory bowel disease (IBD). However, their administration requires a washout period of immunosuppressive drugs that can lead to an exacerbation of the symptoms. Therefore, the feasibility and effects of the combined application of stem cells and prednisone in IBD-dogs without adequate response to corticosteroids was evaluated for the first time in this study over a long- term follow up. Two groups of dogs with IBD, one without treatment and another with prednisone treatment, received a single infusion of stem cells. The clinical indices, albumin and cobalamin were determined prior to the infusion and after one, three, six and 12 months. In both groups, all parameters significantly improved at each time point. In parallel, the steroid dosage was gradually reduced until it was suppressed in all patients a year after the cell therapy. Therefore, cell therapy can significantly and safely improve the disease condition in dogs with IBD receiving or not receiving prednisone. Furthermore, the steroid dosage can be significantly reduced or cancelled after the stem cell infusion. Their beneficial effects are stable over time and are long lasting.
Prenatal Exposure to Innately Preferred D-Limonene and Trans-Anethole Does Not Overcome Innate Aversion to Eucalyptol, Affecting Growth Performance of Weanling Piglets
Authors: Reyes-Camacho David , Pérez José F. , Vinyeta Ester , Aumiller Tobias , Van der Klis Jan D. , Solà-Oriol David
Published: July 2021

In the present research, two studies were performed to determine the effects of specific botanical compounds (BCs) on the innate feed preference and feed intake of piglets, as follows: Exp. 1 studied the innate feed preferences of post-weaning piglets using a double-choice feeding test. A total of 828 weaned piglets were distributed into 36 pens (23 pigs/pen) and assigned to three dietary pair choice feeding options (n = 12): unsupplemented prestarter diets (reference) versus reference plus D-limonene, trans-anethole, or eucalyptol. Piglets showed a preference for diets with D-limonene (53.8%) and trans-anethole (54.5%), and an aversion to eucalyptol (41.6%) (p < 0.05). Exp. 2 studied whether the prenatal and perinatal exposure to D-limonene, trans-anethole, and eucalyptol influences the feed intake and growth of newly-weaned piglets. Twenty-eight gestating and lactating sows were distributed into two dietary treatments (n = 14): unsupplemented Control diets or Control plus a blend of BCs (BBC; containing D-limonene, trans-anethole, and eucalyptol). D-limonene, trans-anethole, and eucalyptol were transferred into the placental fluid, and D-limonene and trans-anethole into the milk (p < 0.05). Furthermore, weanling piglets (n = 200; Control) and (n = 203; BBC) received the same treatment as their mothers in prestarter diets. The early response after weaning showed that piglets’ post-weaning BW gain was higher in the Control (p < 0.05) group than in those exposed to BBC. In conclusion, prenatal exposure to preferred D-limonene and trans-anethole, or familiarity to eucalyptol did not help to overcome the innate aversion to eucalyptol and its negative effect on weanling piglets’ BW.
Detection of 15-bp Deletion Mutation within PLAG1 Gene and Its Effects on Growth Traits in Goats
Authors: Wei Zhenyu , Wang Ke , Wu Hui , Wang Zhen , Pan Chuanying , Chen Hong , Lan Xianyong
Published: July 2021

Stature and weight are important growth and development traits for animals, which also significantly affect the productivity of livestock. Polymorphic adenoma gene 1 (PLAG1) is located in the growth-related quantitative trait nucleotides (QTN), and its variation has been determined to significantly affect the body stature of bovines. This study found that novel 15-bp InDel could significantly influence important growth traits in goats. The frequencies of genotypes of the 15-bp mutation and relationship with core growth traits such as body weight, body height, height at hip cross, chest circumference, hip width and body index were explored in 1581 individuals among 4 Chinese native goat breeds. The most frequent genotypes of Shaanbei white Cashmere goat (SWCG), Inner Mongolia White Cashmere goat (IMCG) and Guanzhong Dairy goat (GZDG) were II genotypes (insertion/insertion), and the frequency of ID genotype (insertion/deletion) was found to be slightly higher than that of II genotype in Hainan Black goat (HNBG), showing that the frequency of the I allele was higher than that of the D allele. In adult goats, there were significant differences between 15-bp variation and body weight, chest circumference and body height traits in SWCG (p < 0.05). Furthermore, the locus was also found to be significantly correlated with the body index of HNBG (p = 0.044) and hip width in GZDG (p = 0.002). In regard to lambs, there were significant differences in height at the hip cross of SWCG (p = 0.036) and hip width in IMWC (p = 0.005). The corresponding results suggest that the 15-bp InDel mutation of PLAG1 is associated with the regulation of important growth characteristics of both adult and lamb of goats, which may serve as efficient molecular markers for goat breeding.
Pressurized Hot Liquid Extraction with 15% v/v Glycerol-Water as An Effective Environment-Friendly Process to Obtain Durvillaea incurvata and Lessonia spicata Phlorotannin Extracts with Antioxidant and Antihyperglycemic Potential
Authors: Erpel Fernanda , Mariotti-Celis María Salomé , Parada Javier , Pedreschi Franco , Pérez-Correa José Ricardo
Published: July 2021

Brown seaweed phlorotannins have shown the potential to promote several health benefits. Durvillaea incurvata and Lessonia spicata—species that are widely distributed in central and southern Chile—were investigated to obtain phlorotannin extracts with antioxidant and antihyperglycemic potential. The use of an environmentally friendly and food-grade glycerol-based pressurized hot liquid extraction (PHLE) process (15% v/v glycerol water) was assessed for the first time to obtain phlorotannins. Multiple effects were analyzed, including the effect of the species, harvesting area (Las Cruces and Niebla), and anatomical part (holdfast, stipe, and frond) on the extracts’ polyphenol content (TPC), antioxidant capacity (AC), and carbohydrate-hydrolyzing enzyme—α-glucosidase and α-amylase—inhibitory activity. Contaminants, such as mannitol, heavy metals (As, Cd, Pb, Hg, and Sn), and 5-hydroxymethylfurfural (HMF), were also determined. The anatomical part used demonstrated a significant impact on the extracts’ TPC and AC, with holdfasts showing the highest values (TPC: 95 ± 24 mg phloroglucinol equivalents/g dry extract; DPPH: 400 ± 140 μmol Trolox equivalents/g dry extract; ORAC: 560 ± 130 μmol TE/g dry extract). Accordingly, holdfast extracts presented the most potent α-glucosidase inhibition, with D. incurvata from Niebla showing an activity equivalent to fifteen times that of acarbose. Only one frond and stipe extract showed significant α-glucosidase inhibitory capacity. No α-amylase inhibition was found in any extract. Although no HMF was detected, potentially hazardous cadmium levels (over the French limit) and substantial mannitol concentrations—reaching up to 50% of the extract dry weight—were found in most seaweed samples and extracts. Therefore, further purification steps are suggested if food or pharmaceutical applications are intended for the seaweed PHLE extracts obtained in this study.
Green One-Pot Synthesis of Coumarin-Hydroxybenzohydrazide Hybrids and Their Antioxidant Potency
Authors: Antonijević Marko R. , Simijonović Dušica M. , Avdović Edina H. , Ćirić Andrija , Petrović Zorica D. , Marković Jasmina Dimitrić , Stepanić Višnja , Marković Zoran S.
Published: July 2021

Compounds from the plant world that possess antioxidant abilities are of special importance for the food and pharmaceutical industry. Coumarins are a large, widely distributed group of natural compounds, usually found in plants, often with good antioxidant capacity. The coumarin-hydroxybenzohydrazide derivatives were synthesized using a green, one-pot protocol. This procedure includes the use of an environmentally benign mixture (vinegar and ethanol) as a catalyst and solvent, as well as very easy isolation of the desired products. The obtained compounds were structurally characterized by IR and NMR spectroscopy. The purity of all compounds was determined by HPLC and by elemental microanalysis. In addition, these compounds were evaluated for their in vitro antioxidant activity. Mechanisms of antioxidative activity were theoretically investigated by the density functional theory approach and the calculated values of various thermodynamic parameters, such as bond dissociation enthalpy, proton affinity, frontier molecular orbitals, and ionization potential. In silico calculations indicated that hydrogen atom transfer and sequential proton loss–electron transfer reaction mechanisms are probable, in non-polar and polar solvents respectively. Additionally, it was found that the single-electron transfer followed by proton transfer was not an operative mechanism in either solvent. The conducted tests indicate the excellent antioxidant activity, as well as the low potential toxicity, of the investigated compounds, which makes them good candidates for potential use in food chemistry.
Enhanced Coupling Coefficient in Dual-Mode ZnO/SiC Surface Acoustic Wave Devices with Partially Etched Piezoelectric Layer
Authors: Xu Huiping , Fu Sulei , Su Rongxuan , Shen Junyao , Zeng Fei , Song Cheng , Pan Feng
Published: July 2021

Surface acoustic wave (SAW) devices based on multi-layer structures have been widely used in filters and sensors. The electromechanical coupling factor (K2), which reflects energy-conversion efficiency, directly determines the bandwidth of the filter and the sensitivity of sensor. In this work, a new configuration of dual-mode (quasi-Rayleigh and quasi-Sezawa) SAW devices on a ZnO/SiC layered structure exhibiting significantly enhanced K2 was studied using the finite element method (FEM), which features in the partial etching of the piezoelectric film between the adjacent interdigitated electrodes (IDTs). The influences of piezoelectric film thickness, etching ratio, top electrodes, bottom electrodes, and the metallization ratio on the K2 were systematically investigated. The optimum K2 for the quasi-Rayleigh mode and quasi-Sezawa mode can exceed 12% and 8%, respectively, which increases by nearly 12 times and 2 times that of the conventional ZnO/SiC structure. Such significantly promoted K2 is of great benefit for better comprehensive performance of SAW devices. More specifically, a quasi-Rayleigh mode with relatively low acoustic velocity (Vp) can be applied into the miniaturization of SAW devices, while a quasi-Sezawa mode exhibiting a Vp value higher than 5000 m/s is suitable for fabricating SAW devices requiring high frequency and large bandwidth. This novel structure has proposed a viable route for fabricating SAW devices with excellent overall performance.
Evaluation of the Absorption of Methionine Carried by Mineral Clays and Zeolites in Porcine Ex Vivo Permeability Models
Authors: Giromini Carlotta , Tretola Marco , Cristiani Cinzia , Finocchio Elisabetta , Silacci Paolo , Panseri Sara , Dell’Anno Matteo , Baldi Antonella , Rossi Luciana
Published: July 2021

Supplemental dietary amino acids (AAs) need to be provided in a form that prevents their degradation along the gastrointestinal tract to guarantee their high bioavailability and bioactivity. In this study, methionine (Met) protected via organo-clay intercalation (natural carriers) has been developed as a sustainable alternative to polymeric coating. Specifically, two different bentonite-zeolite-based mineral clays were tested, Adsorbene (ADS) and BioKi (BIO). Briefly, 1 g of the carrier (ADS or BIO) was contacted with 50 mL of an aqueous solution at a pH of 3.0, 5.8, and 8.9. Solid-liquid separation was conducted. The released Met in the liquid phase was analysed by Chemical Oxygen Demand, while residual Met in the solid phase was analysed by Fourier Transform Infra-Red (FT-IR) spectroscopy. The effect of Met-ADS complex on cell viability was tested on IPEC-J2 cells incubated 3 h with Met-ADS 2.5 mM. Jejunum segments obtained by entire male pigs (Swiss Large White, body weight 100 ± 5 kg) were used as ex vivo models to compare the absorption of 2.5 mM Met released by ADS with 2.5 mM free Met and its influence on epithelial integrity in perfusion Ussing chambers. The carriers released a very low amount of Met and Met-BIO interaction was stronger than Met-ADS. The maximum release of Met was at pH 3, with 3% and 6% of Met release from Met-BIO and Met-ADS, respectively. Cell viability experiments revealed that Met-ADS did not alter cell metabolic activity. No differences in Met absorption and intestinal epithelial integrity were observed ex vivo between free Met and Met-ADS. This study provided new insights into the release of Met from natural clays such as ADS and BIO, the safety of its use in the porcine intestine and the ability of ADS-released Met to absorb to the same extent as the free Met in porcine jejunum.
A Method of Defect Depth Recognition in Active Infrared Thermography Based on GRU Networks
Authors: Xu Li , Hu Jianzhong
Published: July 2021

Active infrared thermography (AIRT) is a significant defect detection and evaluation method in the field of non-destructive testing, on account of the fact that it promptly provides visual information and that the results could be used for quantitative research of defects. At present, the quantitative evaluation of defects is an urgent problem to be solved in this field. In this work, a defect depth recognition method based on gated recurrent unit (GRU) networks is proposed to solve the problem of insufficient accuracy in defect depth recognition. AIRT is applied to obtain the raw thermal sequences of the surface temperature field distribution of the defect specimen. Before training the GRU model, principal component analysis (PCA) is used to reduce the dimension and to eliminate the correlation of the raw datasets. Then, the GRU model is employed to automatically recognize the depth of the defect. The defect depth recognition performance of the proposed method is evaluated through an experiment on polymethyl methacrylate (PMMA) with flat bottom holes. The results indicate that the PCA-processed datasets outperform the raw temperature datasets in model learning when assessing defect depth characteristics. A comparison with the BP network shows that the proposed method has better performance in defect depth recognition.
Evaluation of the SteamVR Motion Tracking System with a Custom-Made Tracker
Authors: Maciejewski Marcin
Published: July 2021

The paper presents the research of the SteamVR tracker developed for a man-portable air-defence training system. The tests were carried out in laboratory conditions, with the tracker placed on the launcher model along with elements ensuring the faithful reproduction of operational conditions. During the measurements, the static tracker was moved and rotated in a working area. The range of translations and rotations corresponded to the typical requirements of a shooting simulator application. The results containing the registered position and orientation values were plotted on 3D charts which showed the tracker’s operation. Further analyses determined the values of the systematic and random errors for measurements of the SteamVR system operating with a custom-made tracker. The obtained results with random errors of 0.15 mm and 0.008° for position and orientation, respectively, proved the high precision of the measurements.
Dietary Habits of Female University Students in Eastern Slovakia in the Context of Sports Activity
Authors: Buková Alena , Zusková Klaudia , Kručanica Ladislav , Küchelová Zuzana , Urbanská Lenka , Melichar Richard
Published: July 2021

(1) Background: The aim of the research was to expand the current knowledge about the diets of female university students in relation to the frequency of their sports activity (SA). We were specifically interested in the eating frequency, the distribution of the amount of food during the day, including breakfast in the diet, and the timing of dinner before bedtime. (2) Methods: The sample in this cross-sectional research consisted of 1055 female freshmen university students from the two universities in eastern Slovakia. Data were obtained through a set of selected questions from a questionnaire battery. (3) Results: We found a significant rank–order correlation between the frequency of meals during the day and the level of SA, as well as between the consumption of breakfast and SA. Furthermore, we found a significant indirect dependence on the distribution of meals during the day and SA. The results indicate a better dietary pattern of the more active female students in terms of the frequency of meals. (4) Conclusions: “The healthy behaviour”, which includes proper diet and regular SA, was monitored in the cross-sectional research we conducted, the results of which consistently point to opportunities for further improvement. There is a need for universities to develop professionally targeted programmes that will enable novice students to modify their health behaviours during their studies.
3D FEA of Infilled RC Framed Structures Protected by Seismic Joints and FRP Jackets
Authors: Rousakis Theodoros , Vanian Vachan , Fanaradelli Theodora , Anagnostou Evgenia
Published: July 2021

This study focused on characteristic cases of recently tested real-scale RC framed wall infilled structures with innovative seismic protection through polyurethane joints (PUFJ) or polyurethane-impregnated fiber grids (FRPU). The frames revealed a highly ductile response while preventing infill collapse. Herein, suitable 3D pseudo-dynamic FE models were developed in order to reproduce the experimental results. The advanced Explicit Dynamics framework may help reveal the unique features of the considered interventions. Externally applied double-sided FRPU jackets on OrthoBlock infills may maintain an adequate bond with the surrounding RC frame as well as with the brick infill substrate at up to a 3.6% drift. In a weak four-column RC structure, the OrthoBlock infills with PUFJ seismic joints may increase the initial stiffness remarkably, increase the base shear by three times (compared with the bare structure) and maintain a high horizontal drift of 3.7%. After this phase, the structure may receive FRPU retrofitting, reveal the redistribution of stress over broad infill regions, including predamaged parts, and still develop a higher initial stiffness and base shear (compared with the bare RC). The realization of a desirable ductile behavior of infilled frames through PUFJ of only 20 mm thickness, as well as through FRPU jacketing, may remarkably broaden the alternatives in seismic protection against the collapse of structures.
Special Issue “Industry 5.0: The Prelude to the Sixth Industrial Revolution”
Authors: Di Nardo Mario , Yu Haoxuan
Published: July 2021

While a significant number of companies around the world are still trying to adapt to Industry 4 [...]
A Bayesian Hierarchical Spatial Copula Model: An Application to Extreme Temperatures in Extremadura (Spain)
Authors: García J. Agustín , Pizarro Mario M. , Acero F. Javier , Parra M. Isabel
Published: July 2021

A Bayesian hierarchical framework with a Gaussian copula and a generalized extreme value (GEV) marginal distribution is proposed for the description of spatial dependencies in data. This spatial copula model was applied to extreme summer temperatures over the Extremadura Region, in the southwest of Spain, during the period 1980–2015, and compared with the spatial noncopula model. The Bayesian hierarchical model was implemented with a Monte Carlo Markov Chain (MCMC) method that allows the distribution of the model’s parameters to be estimated. The results show the GEV distribution’s shape parameter to take constant negative values, the location parameter to be altitude dependent, and the scale parameter values to be concentrated around the same value throughout the region. Further, the spatial copula model chosen presents lower deviance information criterion (DIC) values when spatial distributions are assumed for the GEV distribution’s location and scale parameters than when the scale parameter is taken to be constant over the region.
Combined Production of Astaxanthin and β-Carotene in a New Strain of the Microalga Bracteacoccus aggregatus BM5/15 (IPPAS C-2045) Cultivated in Photobioreactor
Authors: Chekanov Konstantin , Litvinov Daniil , Fedorenko Tatiana , Chivkunova Olga , Lobakova Elena
Published: July 2021

Carotenoids astaxanthin and β-carotene are widely used natural antioxidants. They are key components of functional food, cosmetics, drugs and animal feeding. They hold leader positions on the world carotenoid market. In current work, we characterize the new strain of the green microalga Bracteacoccus aggregatus BM5/15 and propose the method of its culturing in a bubble-column photobioreactor for simultaneous production of astaxanthin and β-carotene. Culture was monitored by light microscopy and pigment kinetics. Fatty acid profile was evaluated by tandem gas-chromatography–mass spectrometry. Pigments were obtained by the classical two-stage scheme of autotrophic cultivation. At the first, vegetative, stage biomass accumulation occurred. Maximum specific growth rate and culture productivity at this stage were 100–200 mg∙L−1∙day−1, and 0.33 day−1, respectively. At the second, inductive, stage carotenoid synthesis was promoted. Maximal carotenoid fraction in the biomass was 2.2–2.4%. Based on chromatography data, astaxanthin and β-carotene constituted 48 and 13% of total carotenoid mass, respectively. Possible pathways of astaxanthin synthesis are proposed based on carotenoid composition. Collectively, a new strain B. aggregatus BM5/15 is a potential biotechnological source of two natural antioxidants, astaxanthin and β-carotene. The results give the rise for further works on optimization of B. aggregatus cultivation on an industrial scale.
Stereotactic Thermal Ablation of Liver Tumors: 3D Planning, Multiple Needle Approach, and Intraprocedural Image Fusion Are the Key to Success—A Narrative Review
Authors: Laimer Gregor , Schullian Peter , Bale Reto
Published: July 2021

Thermal ablation is an emerging, potentially curative approach in treating primary and metastatic liver cancer. Different technologies are available, with radiofrequency ablation (RFA) and microwave ablation (MWA) being the most widely used. Regardless of the technique, destruction of the entire tumor, including an adequate safety margin, is key. In conventional single-probe US- or CT-guided thermal ablation, the creation of such large necrosis zones is often hampered by technical limitations, especially for large tumors (i.e., >2–3 cm). These limitations have been overcome by stereotactic RFA (SRFA): a multiple needle approach with 3D treatment planning and precise stereotactic needle placement combined with intraprocedural image fusion of pre- and post-interventional CT scans for verification of treatment success. With these sophisticated tools and advanced techniques, the spectrum of locally curable liver malignancies can be dramatically increased. Thus, we strongly believe that stereotactic thermal ablation can become a cornerstone in the treatment of liver malignancies, as it offers all the benefits of a minimally invasive method while providing oncological outcomes comparable to surgery. This article provides an overview of current stereotactic techniques for thermal ablation, summarizes the available clinical evidence for this approach, and discusses its advantages.
Characteristics and Survival Outcomes of Hepatocellular Carcinoma Developed after HCV SVR
Authors: Yeh Ming-Lun , Liang Po-Cheng , Tsai Pei-Chien , Wang Shu-Chi , Leong Jennifer , Ogawa Eiichi , Jun Dae Won , Tseng Cheng-Hao , Landis Charles , Tanaka Yasuhito , Huang Chung-Feng , Hayashi Jun , Hsu Yao-Chun , Huang Jee-Fu , Dai Chia-Yen , Chuang Wan-Long , Nguyen Mindie H. , Yu Ming-Lung
Published: July 2021

The clinical presentation and survival of hepatocellular carcinoma (HCC) after hepatitis C virus (HCV) eradication as compared to HCC in viremic patients are not well characterized. We aimed to investigate the characteristics and survival between HCV patients with and without viremia at HCC diagnosis.: We retrospectively analyzed overall survival outcomes in 1389 HCV-related HCC patients, including 301 with HCC developed after HCV eradication (post-SVR HCC) and 1088 with HCV viremia at HCC diagnosis (viremic HCC). We also evaluated overall survival in the two groups using propensity score-matching methods.: At HCC diagnosis, post-SVR HCC patients were older, less obese, less likely cirrhotic, with better liver function, lower alfa-fetoprotein levels, earlier BCLC stages, and higher rate of treatment with surgery. Overall, post-SVR HCC patients had higher median survival than viremic patients (153.3 vs. 55.6 months, p < 0.01), but post-SVR HCC was not independently associated with survival on multivariate analysis (adjusted HR: 1.05, 95% CI: 0.76–1.47). However, on sub-analysis, viremic HCC patients who subsequently received anti-viral treatment and achieved SVR had higher median survival than post-SVR HCC patients (p < 0.01). Viremic HCC with subsequent SVR was also significantly associated with lower mortality as compared to post-SVR HCC (adjusted HR: 0.18, 95% CI: 0.11–0.29). In addition, we observed similar findings in our analysis of the propensity score-matched cohorts.: The advantages in clinical and tumor characters at HCC diagnosis determined the better overall survival of post-SVR HCC patients; however, HCV eradication after HCC development was also associated with improved survival.
NPM1 Mutational Status Underlines Different Biological Features in Pediatric AML
Authors: Tregnago Claudia , Benetton Maddalena , Padrin Davide , Polato Katia , Borella Giulia , Da Ros Ambra , Marchetti Anna , Porcù Elena , Del Bufalo Francesca , Mecucci Cristina , Locatelli Franco , Pigazzi Martina
Published: July 2021

Nucleophosmin (NPM1) is a nucleocytoplasmic shuttling protein, predominantly located in the nucleolus, that regulates a multiplicity of different biological processes. NPM1 localization in the cell is finely tuned by specific signal motifs, with two tryptophan residues (Trp) being essential for the nucleolar localization. In acute myeloid leukemia (AML), several NPM1 mutations have been reported, all resulting in cytoplasmic delocalization, but the putative biological and clinical significance of different variants are still debated. We explored HOXA and HOXB gene expression profile in AML patients and found a differential expression between NPM1 mutations inducing the loss of two (A-like) Trp residues and those determining the loss of one Trp residue (non-A-like). We thus expressed NPM1 A-like- or non-A-like-mutated vectors in AML cell lines finding that NPM1 partially remained in the nucleolus in the non-A-like NPM1-mutated cells. As a result, only in A-like-mutated cells we detected HOXA5, HOXA10, and HOXB5 hyper-expression and p14ARF/p21/p53 pathway deregulation, leading to reduced sensitivity to the treatment with either chemotherapy or Venetoclax, as compared to non-A-like cells. Overall, we identified that the NPM1 mutational status mediates crucial biological characteristics of AML cells, providing the basis for further sub-classification and, potentially, management of this subgroup of patients.
Quality of Automated Stereotactic Radiosurgery Plans in Patients with 4 to 10 Brain Metastases
Authors: Petoukhova Anna , Snijder Roland , Wiggenraad Rudolf , de Boer-de Wit Linda , Mudde-van der Wouden Ivonne , Florijn Mireille , Zindler Jaap
Published: July 2021

The purpose was to compare linac-based stereotactic radiosurgery and hypofractionated radiotherapy plan quality of automated planning, intensity modulated radiotherapy (IMRT) and manual dynamic conformal arc (DCA) plans as well as single- and multiple-isocenter techniques for multiple brain metastases (BM). For twelve patients with four to ten BM, seven non-coplanar linac-based plans were created: a manually planned DCA plan with a separate isocenter for each metastasis, a single-isocenter dynamic IMRT plan, an automatically generated single-isocenter volumetric modulated arc radiotherapy (VMAT) plan, four automatically generated single-isocenter DCA plans with three or five couch angles, with high or low sparing of normal tissue. Paddick conformity index, gradient index (GI), mean dose, total V12Gy and V5Gy of uninvolved brain, number of monitor units (MUs), irradiation time and pass rate were compared. The GI was significantly higher for VMAT than for separate-isocenter, IMRT, and all automatically generated plans. The number of MUs was lowest for VMAT, followed by automatically generated DCA and IMRT plans and highest for manual DCA plans. Irradiation time was the shortest for automatically planned DCA plans. Automatically generated linac-based single-isocenter plans for multiple BM reduce the number of MUs and irradiation time with at least comparable GI and V5Gy relative to the reference separate-isocenter DCA plans.
Time-Sequencing of the Neutrophil-to-Lymphocyte Ratio to Predict Prognosis of Triple-Negative Breast Cancer
Authors: Kim Joo-Heung , Son Nak-Hoon , Lee Jun-Sang , Mun Ji-Eun , Kim Jee-Ye , Park Hyung-Seok , Park Seho , Kim Seung-Il , Park Byeong-Woo
Published: July 2021

Since triple-negative breast cancers (TNBCs) have varying prognoses, it is important to identify subgroups with particularly poor prognosis. The aim of this study was to assess whether changes in the neutrophil-to-lymphocyte ratio (NLR) during the treatment process were associated with poor prognosis in TNBC patients. This study included 600 TNBC patients who underwent surgery from January 2005 to December 2016. The associations of the NLR and clinicopathologic factors with breast cancer recurrence and survival in patients who underwent both definitive local treatment (total mastectomy or breast-conserving surgery with radiotherapy) and systemic chemotherapy were analyzed. The NLRs at four time points (before surgery, before chemotherapy, before radiotherapy, and 1 year after surgery) were assessed. The univariate analysis showed that changes in the NLR before the start of radiotherapy (odds ratio: 1.115, confidence interval: 1.011–1.229) and 1 year after surgery (odds ratio: 1.196, confidence interval: 1.057–1.354) significantly increased the risk of recurrence or death. In multivariate analysis, T stage, N stage, and changes in the NLR were significant factors. A time-sequenced NLR may reflect the prognosis of TNBC patients. A poor prognosis is expected in patients whose NLR increases during treatment compared to the preoperative NLR, and additional treatment is needed.
Abnormal Metabolism in the Progression of Nonalcoholic Fatty Liver Disease to Hepatocellular Carcinoma: Mechanistic Insights to Chemoprevention
Authors: Orabi Danny , Berger Nathan A. , Brown J. Mark
Published: July 2021

Nonalcoholic fatty liver disease (NAFLD) is on the rise and becoming a major contributor to the development of hepatocellular carcinoma (HCC). Reasons for this include the rise in obesity and metabolic syndrome in contrast to the marked advances in prevention and treatment strategies of viral HCC. These shifts are expected to rapidly propel this trend even further in the coming decades, with NAFLD on course to become the leading etiology of end-stage liver disease and HCC. No Food and Drug Administration (FDA)-approved medications are currently available for the treatment of NAFLD, and advances are desperately needed. Numerous medications with varying mechanisms of action targeting liver steatosis and fibrosis are being investigated including peroxisome proliferator-activated receptor (PPAR) agonists and farnesoid X receptor (FXR) agonists. Additionally, drugs targeting components of metabolic syndrome, such as antihyperglycemics, have been found to affect NAFLD progression and are now being considered in the treatment of these patients. As NAFLD drug discovery continues, special attention should be given to their relationship to HCC. Several mechanisms in the pathogenesis of NAFLD have been implicated in hepatocarcinogenesis, and therapies aimed at NAFLD may additionally harbor independent antitumorigenic potential. This approach may provide novel prevention and treatment strategies.
A Switch from Cell-Associated to Soluble PDGF-B Protects against Atherosclerosis, despite Driving Extramedullary Hematopoiesis
Authors: Tillie Renée J. H. A. , Theelen Thomas L. , van Kuijk Kim , Temmerman Lieve , de Bruijn Jenny , Gijbels Marion , Betsholtz Christer , Biessen Erik A. L. , Sluimer Judith C.
Published: July 2021

Platelet-derived growth factor B (PDGF-B) is a mitogenic, migratory and survival factor. Cell-associated PDGF-B recruits stabilizing pericytes towards blood vessels through retention in extracellular matrix. We hypothesized that the genetic ablation of cell-associated PDGF-B by retention motif deletion would reduce the local availability of PDGF-B, resulting in microvascular pericyte loss, microvascular permeability and exacerbated atherosclerosis. Therefore, Ldlr-/-Pdgfbret/ret mice were fed a high cholesterol diet. Although plaque size was increased in the aortic root of Pdgfbret/ret mice, microvessel density and intraplaque hemorrhage were unexpectedly unaffected. Plaque macrophage content was reduced, which is likely attributable to increased apoptosis, as judged by increased TUNEL+ cells in Pdgfbret/ret plaques (2.1-fold) and increased Pdgfbret/ret macrophage apoptosis upon 7-ketocholesterol or oxidized LDL incubation in vitro. Moreover, Pdgfbret/ret plaque collagen content increased independent of mesenchymal cell density. The decreased macrophage matrix metalloproteinase activity could partly explain Pdgfbret/ret collagen content. In addition to the beneficial vascular effects, we observed reduced body weight gain related to smaller fat deposition in Pdgfbret/ret liver and adipose tissue. While dampening plaque inflammation, Pdgfbret/ret paradoxically induced systemic leukocytosis. The increased incorporation of 5-ethynyl-2′-deoxyuridine indicated increased extramedullary hematopoiesis and the increased proliferation of circulating leukocytes. We concluded that Pdgfbret/ret confers vascular and metabolic effects, which appeared to be protective against diet-induced cardiovascular burden. These effects were unrelated to arterial mesenchymal cell content or adventitial microvessel density and leakage. In contrast, the deletion drives splenic hematopoiesis and subsequent leukocytosis in hypercholesterolemia.