bluuebunny/crossref_metadata_2025 huggingface dataset:
Terpenoid Biosynthesis Dominates among Secondary Metabolite Clusters in Mucoromycotina Genomes
Authors: Koczyk Grzegorz , Pawłowska Julia , Muszewska Anna
Published: April 2021

Early-diverging fungi harbour unprecedented diversity in terms of living forms, biological traits and genome architecture. Before the sequencing era, non-Dikarya fungi were considered unable to produce secondary metabolites (SM); however, this perspective is changing. The main classes of secondary metabolites in fungi include polyketides, nonribosomal peptides, terpenoids and siderophores that serve different biological roles, including iron chelation and plant growth promotion. The same classes of SM are reported for representatives of early-diverging fungal lineages. Encouraged by the advancement in the field, we carried out a systematic survey of SM in Mucoromycotina and corroborated the presence of various SM clusters (SMCs) within the phylum. Among the core findings, considerable representation of terpene and nonribosomal peptide synthetase (NRPS)-like candidate SMCs was found. Terpene clusters with diverse domain composition and potentially highly variable products dominated the landscape of candidate SMCs. A uniform low-copy distribution of siderophore clusters was observed among most assemblies. Mortierellomycotina are highlighted as the most potent SMC producers among the Mucoromycota and as a source of novel peptide products. SMC identification is dependent on gene model quality and can be successfully performed on a batch scale with genomes of different quality and completeness.
Internet Usage Habits and Experienced Levels of Psychopathology: A Pilot Study on Association with Spontaneous Eye Blinking Rate
Authors: Simkute Dovile , Nagula Igor , Tarailis Povilas , Burkauskas Julius , Griskova-Bulanova Inga
Published: April 2021

Increasing availability of the internet has resulted in the increased prevalence of problematic online behaviors. Reliable and affordable neurobiological and psychological biomarkers that distinguish problematic internet use (PIU) from functional online activities are of utmost importance. Previous studies have shown a relationship between spontaneous eye blinking rate (sEBR) and changes in dopamine regulation in neurological and psychiatric disorders, including substance use disorders. In this study, we utilized sEBR to examine the potential link between individual differences in dopaminergic neurotransmission and PIU. In sum, 62 subjects participated in this study (median age 25, IQR 6 years, 34 females). The Problematic Internet Use Questionnaire (PIUQ-9), Beck Depression Inventory (BDI-II), Beck Anxiety Inventory (BAI), Clark–Beck Obsessive–Compulsive Inventory (CBOCI) and Barratt Impulsiveness Scale (BIS-11) were used for psychological assessment. The sEBRs were assessed with an electrooculogram recorded from above and below the left eye and from the right and left outer canthi. The group with PIU (PIUQ-9 > 20) expressed higher levels of impulsivity and compulsive behavior symptoms than the control group. In the group with PIU, impulsivity levels were inversely related to sEBR, and a trend of negative association of sEBR with compulsive behavior was observed. Future research should enroll subjects with high levels of PIU and strongly expressed psychopathology levels to further address the utility of sEBR as a potential biomarker.
Fantastic Beasts: Blockchain Based Banking
Authors: Jayasuriya Daluwathumullagamage Dulani , Sims Alexandra
Published: April 2021

Blockchain is one of the primary digital technologies utilised in the finance industry with huge future potential. This study conducts a systematic literature review of a final sample of 407 prior literature from an initial set of 1979 records for the sample period of 2013–2020 with regard to blockchain adoption in banking. This review is further supplemented by a machine learning based textual analysis that identifies key themes, trends, divergences and gaps between academic and practitioner led industry literature. Moreover, the study highlights present, future use cases, adoption barriers and misconceptions of blockchains in banking, especially given COVID-19. Furthermore, this study identifies behavioural, social, economic, regulatory and managerial implications of blockchain based banking. In addition, our study identifies the cross-industry potential of blockchains via banking, thus, linking much disconnected prior literature. Finally, we develop a blockchain adoption framework and an adoption life cycle for banking. This study would be of interest to academics, bankers, regulators, investors, auditors and other stakeholders in financial markets.
How Land Use, Climate Change, and an Ageing Demographic Intersect to Create New Vulnerabilities in Hong Kong
Authors: Kan Wing Shan , Lejano Raul P.
Published: April 2021

As extreme weather events seemingly increase in frequency and magnitude, we are accumulating evidence about how the intersection of circumstances creates vulnerability. The specter of elderly residents in Brooklyn, New York, trapped in their apartments for days due to flooding from the storm surge brought by Hurricane Sandy, provides us a troubling lesson. As vulnerability emerges from the confluence of multiple factors, changing social, natural, and other factors combine to create unimagined problems. Hong Kong is a case in point. The city has seen much of its new development occurring on reclaimed coastal land. At the same time, there has been a significant demographic shift as the city’s elderly population has been its fastest growing demographic. The social transition also means more elderly persons living alone. All of these produce conditions that render the population increasingly vulnerable to coastal flooding. Yet, there is not enough systematic effort, in major cities, at identifying these vulnerabilities. Hong Kong is emblematic of coastal cities the world over, in that it has yet to come to a full realization of such emerging risks. Future research must be able to analyze intersectionalities.
Remote Sensing Analysis to Quantify Change in Woodland Canopy Cover on the San Carlos Apache Reservation, Arizona, USA (1935 vs. 2017)
Authors: Middleton Barry , Norman Laura
Published: April 2021

Since the late 1800s, pinyon–juniper woodland across the western U.S. has increased in density and areal extent and encroached into former grassland areas. The San Carlos Apache Tribe wants to gain qualitative and quantitative information on the historical conditions of their tribal woodlands to use as a baseline for restoration efforts. At the San Carlos Apache Reservation, in east-central Arizona, large swaths of woodlands containing varying mixtures of juniper (Juniperus spp.), pinyon (Pinus spp.) and evergreen oak (Quercus spp.) are culturally important to the Tribe and are a focus for restoration. To determine changes in canopy cover, we developed image analysis techniques to monitor tree and large shrub cover using 1935 and 2017 aerial imagery and compared results over the 82-year interval. Results showed a substantial increase in the canopy cover of the former savannas, and encroachment (mostly juniper) into the former grasslands of Big Prairie. The Tribe is currently engaged in converting juniper woodland back into an open savanna, more characteristic of assumed pre-reservation conditions for that area. Our analysis shows areas on Bee Flat that, under the Tribe’s active restoration efforts, have returned woodland canopy cover to levels roughly analogous to that measured in 1935.
Exploring the Regional Dynamics of U.S. Irrigated Agriculture from 2002 to 2017
Authors: Shrestha Dinesh , Brown Jesslyn F. , Benedict Trenton D. , Howard Daniel M.
Published: April 2021

The United States has a geographically mature and stable land use and land cover system including land used as irrigated cropland; however, changes in irrigation land use frequently occur related to various drivers. We applied a consistent methodology at a 250 m spatial resolution across the lower 48 states to map and estimate irrigation dynamics for four map eras (2002, 2007, 2012, and 2017) and over four 5-year mapping intervals. The resulting geospatial maps (called the Moderate Resolution Imaging Spectroradiometer (MODIS) Irrigated Agriculture Dataset or MIrAD-US) involved inputs from county-level irrigated statistics from the U.S. Department of Agriculture, National Agricultural Statistics Service, agricultural land cover from the U.S. Geological Survey National Land Cover Database, and an annual peak vegetation index derived from expedited MODIS satellite imagery. This study investigated regional and periodic patterns in the amount of change in irrigated agriculture and linked gains and losses to proximal causes and consequences. While there was a 7% overall increase in irrigated area from 2002 to 2017, we found surprising variability by region and by 5-year map interval. Irrigation land use dynamics affect the environment, water use, and crop yields. Regionally, we found that the watersheds with the largest irrigation gains (based on percent of area) included the Missouri, Upper Mississippi, and Lower Mississippi watersheds. Conversely, the California and the Texas–Gulf watersheds experienced fairly consistent irrigation losses during these mapping intervals. Various drivers for irrigation dynamics included regional climate fluctuations and drought events, demand for certain crops, government land or water policies, and economic incentives like crop pricing and land values. The MIrAD-US (Version 4) was assessed for accuracy using a variety of existing regionally based reference data. Accuracy ranged between 70% and 95%, depending on the region.
Moderate Grazer Density Stabilizes Forage Availability More Than Patch Burning in Low-Stature Grassland
Authors: Raynor Edward J. , McGranahan Devan Allen , Miller James R. , Debinski Diane M. , Schacht Walter H. , Engle David M.
Published: April 2021

Spatially patchy fire creates landscape-level diversity that in turn stabilizes several rangeland ecosystem services, including forage production and habitat availability. To enhance biodiversity and livestock production, efforts are underway to restore fire regimes in rangelands throughout the Great Plains. However, invasive species such as tall fescue Schedonorus arundinaceus syn. Festuca arundinacea, initially introduced for forage production, hamper prescribed fire use. Grazer density, or stocking rate, modulates the effect of patchy fire regimes on ecological patterns in invaded, semi-natural rangeland pastures. We compare three diversity–stability responses—temporal variability in aboveground plant biomass, portfolio effects among plant functional groups, and beta diversity in plant functional group composition—in pastures managed with two different fire regimes through three periods of heavy, light, and moderate stocking rate in southern Iowa, USA. Pastures were either burned in patches, with one-third of the pasture burned each year, or completely burned every third year. The period of moderate grazer density had the least temporal variability in aboveground plant biomass, regardless of fire regime. We also found statistical evidence for a portfolio effect under moderate stocking, where diversification of plant communities through varying cover of functional groups can stabilize communities by reducing year-to-year variability. Beta diversity among plant functional groups was greatest during the moderate grazer density period as well. The short stature of tall fescue prevented the patch-burning regime to create contrast in vegetation structure among patches, and there was no difference in any diversity–stability mechanism response across the two different patterns of burning. Although longitudinal, these data suggest that temporal variability in aboveground plant biomass declines with diversity–stability mechanisms that underlie ecosystem function. Our results also support a decades-old principle of range management: moderate grazing intensity enhances diversity and stability, which has been shown to buffer forage shortfalls during drought.
High-Resolution Monitoring and Assessment of Evapotranspiration and Gross Primary Production Using Remote Sensing in a Typical Arid Region
Authors: Yan Junxia , Ma Yanfei , Zhang Dongyun , Li Zechen , Zhang Weike , Wu Zhenhua , Wang Hui , Wen Lihua
Published: April 2021

Land surface evapotranspiration (ET) and gross primary productivity (GPP) are critical components in terrestrial ecosystems with water and carbon cycles. Large-scale, high-resolution, and accurately quantified ET and GPP values are important fundamental data for freshwater resource management and help in understanding terrestrial carbon and water cycles in an arid region. In this study, the revised surface energy balance system (SEBS) model and MOD17 GPP algorithm were used to estimate daily ET and GPP at 100 m resolution based on multi-source satellite remote sensing data to obtain surface biophysical parameters and meteorological forcing data as input variables for the model in the midstream oasis area of the Heihe River Basin (HRB) from 2010 to 2016. Then, we further calculated the ecosystem water-use efficiency (WUE). We validated the daily ET, GPP, and WUE from ground observations at a crop oasis station and conducted spatial intercomparisons of monthly and annual ET, GPP, and WUE at the irrigation district and cropland oasis scales. The site-level evaluation results show that ET and GPP had better performance than WUE at the daily time scale. Specifically, the deviations in the daily ET, GPP, and WUE data compared with ground observations were small, with a root mean square error (RMSE) and mean absolute percent error (MAPE) of 0.75 mm/day and 26.59%, 1.13 gC/m2 and 36.62%, and 0.50 gC/kgH2O and 39.83%, respectively. The regional annual ET, GPP, and WUE varied from 300 to 700 mm, 200 to 650 gC/m2, and 0.5 to 1.0 gC/kgH2O, respectively, over the entire irrigation oasis area. It was found that annual ET and GPP were greater than 550 mm and 500 gC/m2, and annual oasis cropland WUE had strong invariability and was maintained at approximately 0.85 gC/kgH2O. The spatial intercomparisons from 2010 to 2016 revealed that ET had similar spatial patterns to GPP due to tightly coupled carbon and water fluxes. However, the WUE spatiotemporal patterns were slightly different from both ET and GPP, particularly in the early and late growing seasons for the oasis area. Our results demonstrate that spatial full coverage and reasonably fine spatiotemporal variation and variability could significantly improve our understanding of water-saving irrigation strategies and oasis agricultural water management practices in the face of water shortage issues.
A Numerical Model for the Analysis of the Bearings of a Diesel Engine Subjected to Conditions of Wear and Misalignment
Authors: Pardo García Carlos , Rojas Jhan Piero , Orjuela Abril Sofia
Published: April 2021

In the present work, a numerical model is developed to investigate the influence of wear and misalignment on the bearings of a stationary diesel engine. The model implemented considers the effects of surface wear on the bearing, cavitation effects, and surface roughness. For the numerical analysis, changes in the surface roughness of σμσ=0.75 μm, σμσ=1 μm, and σμσ=1.25 μm are defined, and changes in the bearing load of 50%, 75%, and 100%. The results demonstrated that increasing the surface roughness intensifies the bearing wear, which represents 18% and 140% of the bearing clearance for the roughness of σμσ=1 μm and σμσ=1.25 μm, respectively. Additionally, the surface roughness causes a considerable increase in the bearing wear rate. The results described a maximum wear rate of μ20 μm/s. In general, increasing the bearing load by 25% doubles the hydrodynamic pressure conditions increases friction force by 33%, and reduces lubrication film thickness by 12%. The analysis of the angle of deflection, ϕx and ϕy, shows that the moment and the degree of misalignment tend to increase significantly with the increase in the magnitude of the angle ϕy. Negative angles of deflection, ϕx, produce a greater increase in the degree of misalignment and the moment. This implies a greater chance of contact with the bearing surface. In conclusion, the proposed methodology serves as a reliable tool to simultaneously evaluate key parameters on the tribological behavior of bearings that further extend their endurance and minimize wear damage.
Parameter Estimation for Composite Dynamical Systems Based on Sequential Order Statistics from Burr Type XII Mixture Distribution
Authors: Tsai Tzong-Ru , Lio Yuhlong , Xin Hua , Pham Hoang
Published: April 2021

Considering the impact of the heterogeneous conditions of the mixture baseline distribution on the parameter estimation of a composite dynamical system (CDS), we propose an approach to infer the model parameters and baseline survival function of CDS using the maximum likelihood estimation and Bayesian estimation methods. The power-trend hazard rate function and Burr type XII mixture distribution as the baseline distribution are used to characterize the changes of the residual lifetime distribution of surviving components. The Markov chain Monte Carlo approach via using a new Metropolis–Hastings within the Gibbs sampling algorithm is proposed to overcome the computation complexity when obtaining the Bayes estimates of model parameters. A numerical example is generated from the proposed CDS to analyze the proposed procedure. Monte Carlo simulations are conducted to investigate the performance of the proposed methods, and results show that the proposed Bayesian estimation method outperforms the maximum likelihood estimation method to obtain reliable estimates of the model parameters and baseline survival function in terms of the bias and mean square error.
On the Languages Accepted by Watson-Crick Finite Automata with Delays
Authors: Sempere José M.
Published: April 2021

In this work, we analyze the computational power of Watson-Crick finite automata (WKFA) if some restrictions over the transition function in the model are imposed. We consider that the restrictions imposed refer to the maximum length difference between the two input strands which is called the delay. We prove that the language class accepted by WKFA with such restrictions is a proper subclass of the languages accepted by arbitrary WKFA in general. In addition, we initiate the study of the language classes characterized by WKFAs with bounded delays. We prove some of the results by means of various relationships between WKFA and sticker systems.
Limit Theory for Stationary Autoregression with Heavy-Tailed Augmented GARCH Innovations
Authors: Hwang Eunju
Published: April 2021

This paper considers stationary autoregressive (AR) models with heavy-tailed, general GARCH (G-GARCH) or augmented GARCH noises. Limit theory for the least squares estimator (LSE) of autoregression coefficient ρ=ρn is derived uniformly over stationary values in [0,1), focusing on ρn→1 as sample size n tends to infinity. For tail index α∈(0,4) of G-GARCH innovations, asymptotic distributions of the LSEs are established, which are involved with the stable distribution. The convergence rate of the LSE depends on 1−ρn2, but no condition on the rate of ρn is required. It is shown that, for the tail index α∈(0,2), the LSE is inconsistent, for α=2, logn/(1−ρn2)-consistent, and for α∈(2,4), n1−2/α/(1−ρn2)-consistent. Proofs are based on the point process and the asymptotic properties in AR models with G-GARCH errors. However, this present work provides a bridge between pure stationary and unit-root processes. This paper extends the existing uniform limit theory with three issues: the errors have conditional heteroscedastic variance; the errors are heavy-tailed with tail index α∈(0,4); and no restriction on the rate of ρn is necessary.
Cesarean Scar Ectopic Pregnancy—Case Series: Treatment Decision Algorithm and Success with Medical Treatment
Authors: Morente Lorena Sabonet , León Ana I. Guzmán , Reina M. Pilar Espejo , Herrero Jose R. Anderica , Mesa Ernesto González , López Jesús S. Jiménez
Published: April 2021

Background: Cesarean scar ectopic pregnancies are a rare form of extrauterine pregnancies, yet their incidence has increased along with the rise in the number of cesarean deliveries. As with other ectopic pregnancies, cesarean scar ectopic pregnancies pose a greater risk for maternal hemorrhage and ultimately maternal mortality. Case presentation: We present a series of clinical cases of cesarean scar ectopic pregnancy diagnosed by transvaginal ultrasonography. Each patient received an individualized treatment: the rate of success depended on the particular maternal condition in each case. Due to the low frequency of this entity, there are no clear protocols for its treatment and thus there are numerous options for treatment and follow-up: expectant management, medical therapy, surgical intervention, uterine artery embolization or a combined approach. Each method has different levels of success and is dependent on the surgeon’s skill and patient presentation. A transvaginal ultrasound is necessary to obtain the fine details of the gestation sac and its relation to the scar and must be followed by a meticulous abdominal scan with a full bladder. Conclusion: Herein, we present a rare pathological phenomenon whose frequency is on the rise, and for which transvaginal ultrasound and flow Doppler provide high diagnostic accuracy. Early diagnosis of cesarean scar ectopic pregnancies offers treatment options that may help avoid uterine rupture and bleeding, thus preserving the uterus and future fertility.
The Bonar Score in the Histopathological Assessment of Tendinopathy and Its Clinical Relevance—A Systematic Review
Authors: Zabrzyńska Maria , Grzanka Dariusz , Zielińska Wioletta , Jaworski Łukasz , Pękala Przemysław , Gagat Maciej
Published: April 2021

This study aimed to perform a comprehensive systematic review, which reports the role of the Bonar score in the histopathological assessment of tendinopathy and its clinical relevance. To identify all of the studies that reported relevant information on the Bonar scoring system and tendinopathy, an extensive search of the major and the most significant electronic databases (PubMed, Cochrane Central, ScienceDirect, SciELO, Web of Science) was performed. A systematic review of the literature was conducted according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. The extracted data included—year of study, geographical location, type of the study, radiological modifications, gender, number of patients, region of tendinopathy, mean age, control group, characteristics of the Bonar score and alterations in the scale, mean Bonar score, number of investigators, area of tendon investigation, clinical and radiological implications. An extensive search of the databases and other sources yielded a total of 807 articles. Eighteen papers were finally included in this systematic review, and of these, 13 original papers included the clinical and radiological implications of tendinopathy. Radiological evaluation was present in eight studies (both magnetic resonance imaging (MRI) and ultrasound (US)). The clinical implications were more frequent and present in 10 studies. Using the Bonar score, it is easy to quantify the pathological changes in tendinous tissue. However, its connection with clinical and radiological evaluation is much more complicated. Based on the current state of knowledge, we concluded that the neovascularization variable in the Bonar system should be reconsidered. Ideally, the microscopic assessment score should follow the established classification scale with the radiological and clinical agreement and should have a prognostic value.
Is Early Surgical Treatment for Benign Prostatic Hyperplasia Preferable to Prolonged Medical Therapy: Pros and Cons
Authors: Fogaing Cora , Alsulihem Ali , Campeau Lysanne , Corcos Jacques
Published: April 2021

Background and objectives: Treatment of lower urinary tract symptoms (LUTS) related to benign prostatic hyperplasia (BPH) has shifted over the last decades, with medical therapy becoming the primary treatment modality while surgery is being reserved mostly to patients who are not responding to medical treatment or presenting with complications from BPH. Here, we aim to explore the evidence supporting or not early surgical treatment of BPH as opposed to prolonged medical therapy course. Materials and Methods: The debate was presented with a “pro and con” structure. The “pro” side supported the early surgical management of BPH. The “con” side successively refuted the “pro” side arguments. Results: The “pro” side highlighted the superior efficacy and cost-effectiveness of surgery over medical treatment for BPH, as well as the possibility of worse postoperative outcomes for delayed surgical treatment. The “con” side considered that medical therapy is efficient in well selected patients and can avoid the serious risks inherent to surgical treatment of BPH including important sexual side effects. Conclusions: Randomized clinical trials comparing the outcomes for prolonged medical therapy versus early surgical treatment could determine which approach is more beneficial in the long-term in context of the aging population. Until then, both approaches have their advantages and patients should be involve in the treatment decision.
A Novel Electrospinning Polyacrylonitrile Separator with Dip-Coating of Zeolite and Phenoxy Resin for Li-ion Batteries
Authors: Chen Danxia , Wang Xiang , Liang Jianyu , Zhang Ze , Chen Weiping
Published: April 2021

Commercial separators (polyolefin separators) for lithium-ion batteries still have defects such as low thermostability and inferior interface compatibility, which result in serious potential safety distress and poor electrochemical performance. Zeolite/Polyacrylonitrile (Z/PAN) composite separators have been fabricated by electrospinning a polyacrylonitrile (PAN) membrane and then dip-coating it with zeolite (ZSM-5). Different from commercial separators, the Z/PAN composite separators exhibit high electrolyte uptake, excellent ionic conductivity, and prominent thermal stability. Specifically, the Z/PAN-1.5 separator exhibits the best performance, with a high electrolyte uptake of 308.1% and an excellent ionic conductivity of 2.158 mS·cm−1. The Z/PAN-1.5 separator may mechanically shrink less than 10% when held at 180 °C for 30 min, proving good thermal stability. Compared with the pristine PAN separator, the Li/separator/LiFePO4 cells with the Z/PAN-1.5 composite separator have excellent high-rate discharge capacity (102.2 mAh·g−1 at 7 C) and favorable cycling performance (144.9 mAh·g−1 at 0.5 C after 100 cycles). Obviously, the Z/PAN-1.5 separator holds great promise in furthering the safety and performance of lithium-ion batteries.
Ultra-High Packing Density Next Generation Microtube Array Membrane for Absorption Based Applications
Authors: Chew Chee Ho , Huang Wan-Ting , Yang Tzu-Sen , Chen Amanda , Wu Yun Ming , Wu Mai-Szu , Chen Chien-Chung
Published: April 2021

Previously, we successfully developed an extracorporeal endotoxin removal device (EERD) that is based on the novel next generation alternating microtube array membrane (MTAM-A) that was superior to the commercial equivalent. In this article, we demonstrated multiple different parameter modifications that led to multiple different types of novel new MTAM structures, which ultimately led to the formation of the MTAM-A. Contrary to the single layered MTAM, the MTAM-A series consisted of a superior packing density fiber connected in a double layered, alternating position which allowed for the greater fiber count to be packed per unit area. The respective MTAM variants were electrospun by utilizing our internally developed tri-axial electrospinning set up to produce the novel microstructures as seen in the respective MTAM variants. A key uniqueness of this study is the ability to produce self-arranged fibers into the respective MTAM variants by utilizing a single spinneret, which has not been demonstrated before. Of the MTAM variants, we observed a change in the microstructure from a single layered MTAM to the MTAM-A series when the ratio of surfactant to shell flow rate approaches 1:1.92. MTAM-A registered the greatest surface area of 2.2 times compared to the traditional single layered MTAM, with the greatest tensile strength at 1.02 ± 0.13 MPa and a maximum elongation of 57.70 ± 9.42%. The MTAM-A was selected for downstream immobilization of polymyxin B (PMB) and assembly into our own internally developed and fabricated dialyzer housing. Subsequently, the entire setup was tested with whole blood spiked with endotoxin; and benchmarked against commercial Toraymyxin fibers of the same size. The results demonstrated that the EERD based on the MTAM-A performed superior to that of the commercial equivalent, registering a rapid reduction of 73.18% of endotoxin (vs. Toraymyxin at 38.78%) at time point 15 min and a final total endotoxin removal of 89.43% (vs. Toraymyxin at 65.03%).
Effect of Aging Time on Crushing Performance of Al-0.5Mg-0.4Si Alloy for Safety Components of Automobile
Authors: Lu Jinkun , Jiang Haichun , Ye Lingying , Sun Daxiang , Zhang Yong , Zhan Xin , Li Yuping , Ma Lehang , Ran Qingrong , Tang Jianguo
Published: April 2021

The effect of aging time on the crushing performance of Al-0.5Mg-0.4Si alloy used for safety components of automobile was investigated by tensile test and crush test. Moreover, the microstructure of the alloy was investigated by transmission electron microscopy (TEM). The results show that the localized deformation ductility index, ΔAabs, which is defined as the difference between total elongation and uniform elongation, of Al-0.5Mg-0.4Si alloy is 6.5%, 7.0% and 8.5%, respectively, after being aged at 210 °C for 1, 3 and 6 h, and this tendency is the same as that of the crushing performance. The spacing of grain boundary precipitates (GBPs) from TEM results are found to be 94.9, 193.6 and 408.2 nm after being aged at 210 °C for 1, 3 and 6 h, respectively, and this tendency is same to that of ΔAabs. A mechanism about the relation between the spacing of GBPs and the ductility index ΔAabs has been proposed based on localized deformation around GBPs. With the increase of GBPs spacing, the ΔAabs increases, and the crushing performance is improved.
Pinus pinaster Early Hormonal Defence Responses to Pinewood Nematode (Bursaphelenchus xylophilus) Infection
Authors: Rodrigues Ana M. , Langer Swen , Carrasquinho Isabel , Bergström Ed , Larson Tony , Thomas-Oates Jane , António Carla
Published: April 2021

The pinewood nematode (PWN) is the causal agent of pine wilt disease, a pathology that affects conifer forests, mainly Pinus spp. PWN infection can induce the expression of phytohormone-related genes; however, changes at the early phytohormone level have not yet been explored. Phytohormones are low-abundance metabolites, and thus, difficult to quantify. Moreover, most methodologies focus mainly on Arabidopsis or crop species. This work aimed to validate a fast (run time 6.6 min) liquid chromatography-triple quadrupole tandem mass spectrometry (LC-QqQ-MS/MS) analytical method to quantify 14 phytohormones in Pinus pinaster stem tissues. This method was further applied to evaluate, for the first time, early phytohormone changes in susceptible and resistant phenotypes of P. pinaster 24, 48 and 72 h after inoculation (HAI) with PWN. A significant increase in salicylic acid (SA, 48 and 72 HAI) and jasmonic acid methyl ester (JA-ME, 72 HAI) was observed in susceptible phenotypes. Results indicate that the higher susceptibility of P. pinaster to PWN infection might result from an inefficient trigger of hypersensitive responses, with the involvement of JA and SA pathways. This work provides an important update in forest research, and adds to the current knowledge of Pinus spp. defence responses to PWN infection.
Plasma Metabolome and Lipidome Associations with Type 2 Diabetes and Diabetic Nephropathy
Authors: Tan Yan Ming , Gao Yan , Teo Guoshou , Koh Hiromi W.L. , Tai E Shyong , Khoo Chin Meng , Choi Kwok Pui , Zhou Lei , Choi Hyungwon
Published: April 2021

We conducted untargeted metabolomics analysis of plasma samples from a cross-sectional case–control study with 30 healthy controls, 30 patients with diabetes mellitus and normal renal function (DM-N), and 30 early diabetic nephropathy (DKD) patients using liquid chromatography-mass spectrometry (LC-MS). We employed two different modes of MS acquisition on a high-resolution MS instrument for identification and semi-quantification, and analyzed data using an advanced multivariate method for prioritizing differentially abundant metabolites. We obtained semi-quantification data for 1088 unique compounds (~55% lipids), excluding compounds that may be either exogenous compounds or treated as medication. Supervised classification analysis over a confounding-free partial correlation network shows that prostaglandins, phospholipids, nucleotides, sugars, and glycans are elevated in the DM-N and DKD patients, whereas glutamine, phenylacetylglutamine, 3-indoxyl sulfate, acetylphenylalanine, xanthine, dimethyluric acid, and asymmetric dimethylarginine are increased in DKD compared to DM-N. The data recapitulate the well-established plasma metabolome changes associated with DM-N and suggest uremic solutes and oxidative stress markers as the compounds indicating early renal function decline in DM patients.
Metabolic Profile Discriminates and Predicts Arabidopsis Susceptibility to Virus under Field Conditions
Authors: Rubio Bernadette , Fernandez Olivier , Cosson Patrick , Berton Thierry , Caballero Mélodie , Lion Roxane , Roux Fabrice , Bergelson Joy , Gibon Yves , Schurdi-Levraud Valérie
Published: April 2021

As obligatory parasites, plant viruses alter host cellular metabolism. There is a lack of information on the variability of virus-induced metabolic responses among genetically diverse plants in a natural context with daily changing conditions. To decipher the metabolic landscape of plant-virus interactions in a natural setting, twenty-six and ten accessions of Arabidopsis thaliana were inoculated with Turnip mosaic virus (TuMV), in two field experiments over 2 years. The accessions were measured for viral accumulation, above-ground biomass, targeted and untargeted metabolic profiles. The phenotypes of the accessions ranged from susceptibility to resistance. Susceptible and resistant accessions were shown to have different metabolic routes after inoculation. Susceptible genotypes accumulate primary and secondary metabolites upon infection, at the cost of hindered growth. Twenty-one metabolic signatures significantly accumulated in resistant accessions whereas they maintained their growth as mock-inoculated plants without biomass penalty. Metabolic content was demonstrated to discriminate and be highly predictive of the susceptibility of inoculated Arabidopsis. This study is the first to describe the metabolic landscape of plant-virus interactions in a natural setting and its predictive link to susceptibility. It provides new insights on plant-virus interactions. In this undomesticated species and in ecologically realistic conditions, growth and resistance are in a permanent conversation.
Profile of Stilbenes and Other Phenolics in Fanagoria White and Red Russian Wines
Authors: Suprun Andrey R. , Dubrovina Alexandra S. , Tyunin Alexey P. , Kiselev Konstantin V.
Published: April 2021

Grapes and wines represent the most important source of edible stilbenes and other phenolic metabolites, which demonstrate a wide range of valuable biological activities. However, there is no information about the profile and content of phenolic compounds in Russian wines. We firstly analyzed phenolics (stilbenes, phenolic acids, and flavonols) in some representatives of Russian wines, including eleven red and seven white Russian wines from Fanagoria, Krasnodarsky Territory. The Russian red wines contained six stilbenes (trans-resveratrol, cis-resveratrol, trans-, cis-piceid, trans-piceatannol, δ-viniferin), while the white wines contained only five stilbenes (cis-resveratrol, trans-, cis-piceid, trans-piceatannol, trans-resveratrol). More than a half of the total stilbenes in the wines (65% of all stilbenes) were presented by trans-piceid and cis-piceid, while trans-resveratrol reached 16% of all the stilbenes. The red wines also contained six phenolic acids and six flavonols, while the white wines contained six phenolic acids and only three flavonols. Myrecitin-3-O-glucoside, quercetin-3-O-glucoside, and myricetin were the major flavonols in the red wines, while dihydroquercetin-3-O-rhamnoside was the major flavonol in the white wines. The red wines contained markedly higher amounts of stilbenes, phenolic acids, and flavonols than the white wines. Thus, the data showed that young red Russian Fanagoria wines represent a rich source of phenolic compounds. The study also revealed that younger wines were more abundant in phenolics, and wine storage for six months in the dark at +10 °C led to a decrease in the total content of phenolics, primarily monomeric stilbenes and quercetin-3-O-glucoside and quercetin flavonols.
Review of the Newly Developed, Mobile Optical Sensors for Real-Time Measurement of the Atmospheric Particulate Matter Concentration
Authors: Molaie Sama , Lino Paolo
Published: April 2021

Due to the adverse effects on human health and the environment, air quality monitoring, specifically particulate matter (PM), has received increased attention over the last decades. Most of the research and policy actions have been focused on decreasing PM pollution and the development of air monitoring technologies, resulting in a decline of total ambient PM concentrations. For these reasons, there is a continually increasing interest in mobile, low-cost, and real-time PM detection instruments in both indoor and outdoor environments. However, to the best of the authors’ knowledge, there is no recent literature review on the development of newly designed mobile and compact optical PM sensors. With this aim, this paper gives an overview of the most recent advances in mobile optical particle counters (OPCs) and camera-based optical devices to detect particulate matter concentration. Firstly, the paper summarizes the particulate matter effects on human health and the environment and introduces the major particulate matter classes, sources, and characteristics. Then, it illustrates the different theories, detection methods, and operating principles of the newly developed portable optical sensors based on light scattering (OPCs) and image processing (camera-based sensors), including their advantages and disadvantages. A discussion concludes the review by comparing different novel optical devices in terms of structures, parameters, and detection sensitivity.
Safety Evaluation, Biogenic Amine Formation, and Enzymatic Activity Profiles of Autochthonous Enterocin-Producing Greek Cheese Isolates of the Enterococcus faecium/durans Group
Authors: Tsanasidou Charikleia , Asimakoula Stamatia , Sameli Nikoletta , Fanitsios Christos , Vandera Elpiniki , Bosnea Loulouda , Koukkou Anna-Irini , Samelis John
Published: April 2021

Autochthonous single (Ent+) or multiple (m-Ent+) enterocin-producing strains of dairy enterococci show promise for use as bioprotective adjunct cultures in traditional cheese technologies, provided they possess no pathogenic traits. This study evaluated safety, decarboxylase activity, and enzymatic (API ZYM) activity profiles of nine Ent+ or m-Ent+ Greek cheese isolates previously assigned to four distinct E. faecium (represented by the isolates KE64 (entA), GL31 (entA), KE82 (entA-entB-entP) and KE77 (entA-entB-entP-bac31)) and two E. durans (represented by the isolates KE100 (entP) and KE108 (entP-bac31-cyl)) strain genotypes. No strain was β-hemolytic or harbored vanA and vanB or the virulence genes agg, ace, espA, IS16, hyl, or gelE. All strains were of moderate to high sensitivity to ampicillin, ciprofloxacin, chloramphenicol, erythromycin, gentamicin, penicillin, tetracycline, and vancomycin, except for the E. faecium KE64 and KE82 strains, which were resistant to erythromycin and penicillin. All cheese strains showed moderate to strong esterase-lipase and aminopeptidase activities and formed tyramine, but none formed histamine in vitro. In conclusion, all Ent+ or m-Ent+ strain genotypes of the E. faecium/durans group, except for the cyl-positive E. durans KE108, were safe for use as adjunct cultures in traditional Greek cheeses. Further in situ biotechnological evaluations of the strains in real cheese-making trials are required.
The Nonribosomal Peptide Valinomycin: From Discovery to Bioactivity and Biosynthesis
Authors: Huang Shuhui , Liu Yushi , Liu Wan-Qiu , Neubauer Peter , Li Jian
Published: April 2021

Valinomycin is a nonribosomal peptide that was discovered from Streptomyces in 1955. Over the past more than six decades, it has received continuous attention due to its special chemical structure and broad biological activities. Although many research papers have been published on valinomycin, there has not yet been a comprehensive review that summarizes the diverse studies ranging from structural characterization, biogenesis, and bioactivity to the identification of biosynthetic gene clusters and heterologous biosynthesis. In this review, we aim to provide an overview of valinomycin to address this gap, covering from 1955 to 2020. First, we introduce the chemical structure of valinomycin together with its chemical properties. Then, we summarize the broad spectrum of bioactivities of valinomycin. Finally, we describe the valinomycin biosynthetic gene cluster and reconstituted biosynthesis of valinomycin. With that, we discuss possible opportunities for the future research and development of valinomycin.
Different Responses of Bacterial and Archaeal Communities in River Sediments to Water Diversion and Seasonal Changes
Authors: Lv Jiali , Niu Yangdan , Yuan Ruiqiang , Wang Shiqin
Published: April 2021

In recent years, different responses of archaea and bacteria to environmental changes have attracted increasing scientific interest. In the mid-latitude region, Fen River receives water transferred from the Yellow River, electrical conductivity (EC), concentrations of Cl− and Na+ in water, total phosphorus (TP), and Olsen phosphorus (OP) in sediments were significantly affected by water transfer. Meanwhile, temperature and oxidation-reduction potential (ORP) of water showed significant seasonal variations. Based on 16S rRNA high-throughput sequencing technology, the composition of bacteria and archaea in sediments was determined in winter and summer, respectively. Results showed that the dominance of bacterial core flora decreased and that of archaeal core flora increased after water diversion. The abundance and diversity of bacterial communities in river sediments were more sensitive to anthropogenic and naturally induced environmental changes than that of archaeal communities. Bacterial communities showed greater resistance than archaeal communities under long-term external disturbances, such as seasonal changes, because of rich species composition and complex community structure. Archaea were more stable than bacteria, especially under short-term drastic environmental disturbances, such as water transfer, due to their insensitivity to environmental changes. These results have important implications for understanding the responses of bacterial and archaeal communities to environmental changes in river ecosystems affected by water diversion.
Extremofiles 2.0
Authors: Amils Ricardo , Gómez Felipe
Published: April 2021

The exploration of extreme environments has led to the discovery of numerous environments that were, until recently, considered uninhabitable [...]
In Situ High-Temperature X-ray Powder Diffraction and Infrared Spectroscopic Study of Melanterite, FeSO4·7H2O
Authors: Lacalamita Maria , Ventruti Gennaro , Della Ventura Giancarlo , Radica Francesco , Mauro Daniela , Schingaro Emanuela
Published: April 2021

The thermal behavior of melanterite from the Fornovolasco mine (Tuscany, Italy) has been investigated via differential thermal analysis (DTA), thermogravimetry (TG), in situ high-temperature X-ray powder diffraction (XRPD) and Fourier-transform infrared spectroscopy (FTIR). The DTA curve showed endothermic peaks at 70, 100, 260, 500–560 and 660 °C whereas the TG curve evidenced a total mass decrease of ~68%, in keeping with the loss of all H2O and SO4 groups. Rietveld refinements were performed for all the collected patterns in the 25–775 °C range and converged at 1.57 ≤ R (%) ≤ 2.75 and 1.98 ≤ Rwp (%) ≤ 3.74. The decomposition steps FeSO4·7H2O → FeSO4·4H2O (25 ≤ T ≤ 50 °C) → FeSO4·H2O (50 < T ≤ 100 °C) → FeOHSO4 (75 < T ≤ 200 °C) → Fe2(SO4)3 (400 < T ≤ 500 °C) → Fe2O3 (500 < T ≤ 775 °C) were obtained. The high-temperature infrared analysis confirmed that melanterite undergoes a three-step dehydration in the 25–300 °C temperature range. The FeOHSO4 phase is stable over a wide range of temperature and transforms partially to Fe2(SO4)3 without the formation of Fe2O(SO4)2. The findings highlight a different behavior of the studied sample with respect to the synthetic salt.
Algae-Derived Bioactive Molecules for the Potential Treatment of SARS-CoV-2
Authors: Alam Md. Asraful , Parra-Saldivar Roberto , Bilal Muhammad , Afroze Chowdhury Alfi , Ahmed Md. Nasir , Iqbal Hafiz M.N. , Xu Jingliang
Published: April 2021

The recently emerged COVID-19 disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has adversely affected the whole world. As a significant public health threat, it has spread worldwide. Scientists and global health experts are collaborating to find and execute speedy diagnostics, robust and highly effective vaccines, and therapeutic techniques to tackle COVID-19. The ocean is an immense source of biologically active molecules and/or compounds with antiviral-associated biopharmaceutical and immunostimulatory attributes. Some specific algae-derived molecules can be used to produce antibodies and vaccines to treat the COVID-19 disease. Algae have successfully synthesized several metabolites as natural defense compounds that enable them to survive under extreme environments. Several algae-derived bioactive molecules and/or compounds can be used against many diseases, including microbial and viral infections. Moreover, some algae species can also improve immunity and suppress human viral activity. Therefore, they may be recommended for use as a preventive remedy against COVID-19. Considering the above critiques and unique attributes, herein, we aimed to systematically assess algae-derived, biologically active molecules that could be used against this disease by looking at their natural sources, mechanisms of action, and prior pharmacological uses. This review also serves as a starting point for this research area to accelerate the establishment of anti-SARS-CoV-2 bioproducts.
Application of Capillary Electrophoresis to the Analysis of Bioactive Compounds in Herbal Raw Materials
Authors: Przybylska Anna , Gackowski Marcin , Koba Marcin
Published: April 2021

The article is a summary of scientific reports from the last 16 years (2005–2021) on the use of capillary electrophoresis to analyze polyphenolic compounds, coumarins, amino acids, and alkaloids in teas or different parts of plants used to prepare aqueous infusions, commonly known as “tea” or decoctions. This literature review is based on PRISMA guidelines and articles selected in base of criteria carried out using PICOS (Population, Intervention, Comparison, Outcome, Study type). The analysis showed that over 60% of articles included in this manuscript comes from China. The literature review shows that for the selective electrophoretic separation of polyphenolic and flavonoid compounds, the most frequently used capillary electromigration technique is capillary electrophoresis with ultraviolet detection. Nevertheless, the use of capillary electrophoresis-mass spectrometry allows for the sensitive determination of analytes with a lower limit of detection and gives hope for routine use in the analysis of functional foods. Moreover, using the modifications in electrochemical techniques allows methods sensitivity reduction along with the reduction of analysis time.