bluuebunny/crossref_metadata_2025 huggingface dataset:
Numerical Simulations on Unsteady Nonlinear Transonic Airfoil Flow
Authors: Friedewald Diliana
Published: December 2020

Large-amplitude excitations need to be considered for gust load analyses of transport aircraft in cruise flight conditions. Nonlinear amplitude effects in transonic flow are, however, only marginally taken into account. The present work aims at closing this gap by means of systematic unsteady Reynolds-averaged Navier-Stokes simulations. The RAE2822 airfoil is analyzed for a variety of sinusoidal gust excitations at different transonic Mach numbers. Responses are evaluated with respect to lift and moment coefficients, their derivatives and the unsteady shock motion. A strong dependency on inflow Mach number and excitation frequency is observed. Generally, amplitude effects decrease with lower Mach numbers or higher excitation frequencies. The unsteady nonlinear simulations predict lower maximum lift values and lower lift and moment derivatives compared to their linear counterparts for lower frequencies in combination with large-amplitude excitations. For the mid-frequency range, trends are not as clear. Additionally, it is shown that the variables of harmonic distortion and maximum shock motion might not be reasonable indicators to predict a nonlinear response.
Sustainable Business Model Innovation vs. “Made in” for International Performance of Italian Food Companies
Authors: De Chiara Alessandra
Published: December 2020

The quality of Italian food products, linked to Made in, has always been a competitive driver within foreign markets. However, today, getting quality choices also means engaging in responsible behavior. The paper investigates the relation between the choice of environmental and social standards and the international performance of a set of agri-food firms in Italy, examined through the multiple case study method and the tools of qualitative methodology. What role do standards play in attributing an added value to the quality of agroindustry products and differentiating sustainable products in foreign markets, thereby improving the international performance of the companies? These questions are investigated by the research in this paper. The results of the research show a significant correlation, in the interviewed companies, between corporate social responsibility (CSR) practices, with reference to the adoption of standards, and international competitiveness, measured in terms of market performance as it regards the growth of foreign demand and opening to new markets.
Analytical Concentrations of Some Elements in Seeds and Crude Extracts from Aesculus hippocastanum, by ICP-OES Technique
Authors: Durante Caterina , Cocchi Marina , Lancellotti Lisa , Maletti Laura , Marchetti Andrea , Roncaglia Fabrizio , Sighinolfi Simona , Tassi Lorenzo
Published: December 2020

The metal content in some samples of horse chestnut seeds (Aesculus hippocastanum) was monitored over time (years 2016–2019) considering the two most common and representative Mediterranean varieties: the pure species (AHP, which gives white flowers) and a hybrid one (AHH, which gives pink flowers). The selected elemental composition of the samples was determined by applying the Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES) technique. Several samples obtained from different preliminary treatments of the peeled seeds were examined, such as: (i) floury samples (wild-type) mineralized with the wet method; (ii) the ashes of both AHP and AHH varieties; (iii) the fraction of total inorganic soluble salts (TISS). Furthermore, the hydroalcoholic crude extracts (as a tincture) were obtained according to the official Pharmacopoeia methods, and the relevant results were compared with those of a commercial sample, an herbal product-food supplement of similar characteristics. The main characteristics of this research work underline that the two botanical varieties give different distinctive characters, due to the Fe content (80.05 vs. 1.42 mg/100 g d.s., for AHP and AHH wild-type flour samples, respectively), along with K, Ca, Mn, Ni and Cu, which are more abundant in the AHP samples. Furthermore, the Principal Component Analysis (PCA) was applied to the experimental dataset in order to classify and discriminate the samples, in relation to their similar botanical origin, but different for the color of the bloom. These results can be useful for the traceability of raw materials potentially intended for the production of auxiliary systems of pharmacological interest.
Temporal Variations of Soil Organic Carbon and pH at Landscape Scale and the Implications for Cropping Intensity in Rice-Based Cropping Systems
Authors: Siddique Md. Noor E. Alam , Lobry de Bruyn Lisa , Guppy Chris N. , Osanai Yui
Published: December 2020

Landscape scale assessment of temporal variations in soil organic carbon (SOC) contents and soil pH and the implications for long-term agricultural sustainability was determined using legacy datasets collected over two periods separated by 20 years: the 1990s and 2010s. Soil data on SOC and pH were categorized according to the prevailing land types (based on inundation as highland (HL), medium highland (MHL), and medium lowland (MLL)), and physiographic types (i.e., Himalayan Piedmont plain, Tista Floodplain and Barind tract/Terrace) to determine which variable or combination of variables was more influential in spatial and temporal changes of these properties. SOC contents in the physiographic types were generally found to be low, varying between 8 to 12 g/kg. While, SOC contents were significantly higher in MHL and MLL compared with HL that experienced less inundation. The change in SOC contents over 20 years was significant with a 14.5% increase of SOC. There was a greater influence of land type compared with physiography on SOC contents over time. Inundation land types and associated cropping intensity were considered likely to influence SOC of soils under rice-based cropping systems. Furthermore, the levels of soil pH decreased by 0.5 units over 20 years with an approximately 50% increase in soils within a pH category of 4.6–5.5. The majority of soil pH results shift from slightly acidic to strongly acidic in the intervening 20-year period between samplings. Soil acidification is potentially a combination of inefficient and excess use of ammonium-based fertilizers with higher application rates and low input from residues. We conclude that acidification may continue with more intensive land use. However, trends in SOC contents over time under certain combinations of physiography and land type either increased slightly or showed a significant loss and in the latter, specifically, the role of land management is not clear. The legacy datasets would be useful for monitoring spatial and temporal soil quality trends at a regional scale, but has limited capacity to capture field level variations in soil properties as data on smallholder cropping practice and management were not collected. Therefore, future research examining the role of management in SOC and pH dynamics at the field-scale would guide the use of fertilizers, crop residue management, and amelioration of acidic soil, to improve the sustainability of rice-based cropping systems in Bangladesh.
Emerging Genetic Tools to Investigate Molecular Pathways Related to Heat Stress in Chickens: A Review
Authors: Perini Francesco , Cendron Filippo , Rovelli Giacomo , Castellini Cesare , Cassandro Martino , Lasagna Emiliano
Published: December 2020

Chicken products are the most consumed animal-sourced foods at a global level across greatly diverse cultures, traditions, and religions. The consumption of chicken meat has increased rapidly in the past few decades and chicken meat is the main animal protein source in developing countries. Heat stress is one of the environmental factors which decreases the productive performance of poultry and meat quality. Heat stress produces the over-expression of heat shock factors and heat shock proteins in chicken tissues. Heat shock proteins regulate several molecular pathways in cells in response to stress conditions, changing the homeostasis of cells and tissues. These changes can affect the physiology of the tissue and hence the production ability of chickens. Indeed, commercial chicken strains can reach a high production level, but their body metabolism, being comparatively accelerated, has poor thermoregulation. In contrast, native backyard chickens are more adapted to the environments in which they live, with a robustness that allows them to survive and reproduce constantly. In the past few years, new molecular tools have been developed, such as RNA-Seq, Single Nucleotide Polymorphisms (SNPs), and bioinformatics approaches such as Genome-Wide Association Study (GWAS). Based on these genetic tools, many studies have detected the main pathways involved in cellular response mechanisms. In this context, it is necessary to clarify all the genetic and molecular mechanisms involved in heat stress response. Hence, this paper aims to review the ability of the new generation of genetic tools to clarify the molecular pathways associated with heat stress in chickens, offering new perspectives for the use of these findings in the animal breeding field.
Dietary Black Seed Effects on Growth Performance, Proximate Composition, Antioxidant and Histo-Biochemical Parameters of a Culturable Fish, Rohu (Labeo rohita)
Authors: Latif Maria , Faheem Mehwish , Asmatullah  , Hoseinifar Seyed Hossein , Van Doan Hien
Published: December 2020

This feeding trial was conducted to investigate the effects of dietary black seed (Nigella sativa) supplementation on the growth performance, muscles proximate composition, antioxidant and histo-biochemical parameters of rohu (Labeo rohita). Fingerlings (8.503 ± 0.009 g) were fed on 0.0%, 1% and 2.5% black seed supplemented diets for 28 days. Fish sampling was done on the 7th, 14th, 21st and 28th day of experiment. The results of the present study indicated that black seed supplementation significantly increased growth performance and muscles protein contents of rohu over un-supplemented ones. Lipid peroxidation levels significantly decreased in all the studied tissues (liver, gills, kidney and brain) of black seed fed rohu, whereas the antioxidant enzymes (catalase, glutathione-S-transferase, glutathione peroxidase and reduced glutathione) activities were increased in all the studied tissues of black seed supplemented rohu at each sampling day. The hepatic-nephric marker enzymes levels were decreased for black seed fed rohu. The present study showed that tested black seed levels are safe for rohu. Black seed is cheaply available in local markets of Pakistan; therefore, based on the results of the present study, it is suggested that black seed has potential to be used as natural growth promoter and antioxidant in the diet of rohu.
Transcriptome Analysis of Porcine Granulosa Cells in Healthy and Atretic Follicles: Role of Steroidogenesis and Oxidative Stress
Authors: Meng Li , Wu Zhenfang , Zhao Kun , Tao Jian , Chit Tam , Zhang Shouquan , Wang Chi Chiu , Teerds Katja
Published: December 2020

One of the main causes of female infertility is a deregulated antral follicular atresia, a process of which the underlying molecular mechanisms are largely unknown. Our objective was therefore to characterize the complex transcriptome changes in porcine granulosa cells of healthy antral (HA) and advanced antral atretic (AA) follicles, using ELISA and RNA-Seq followed by qRT-PCR and immunohistochemistry. Granulosa cell RNA-Seq data revealed 2160 differentially expressed genes, 1483 with higher and 677 with lower mRNA concentrations in AA follicles. Bioinformatic analysis showed that the upregulated genes in AA follicles were highly enriched in inflammation and apoptosis processes, while the downregulated transcripts were mainly highlighted in the steroid biosynthesis pathway and response to oxidative stress processes including antioxidant genes (e.g., GSTA1, GCLC, GCLM, IDH1, GPX8) involved in the glutathione metabolism pathway and other redox-related genes (e.g., RRM2B, NDUFS4). These observations were confirmed by RT-qPCR and immunohistochemistry. Additionally, the granulosa cells of AA follicles express significantly stronger 8-OHdG immunostaining, a marker of oxidative DNA damage, implicating that oxidative stress may participate in follicular atresia. We hypothesize that the decrease in anti-apoptotic factors and steroid hormones coincides with increased oxidative stress markers and the expression of pro-apoptotic factors, all contributing to antral follicular atresia.
Can Walnut Serve as a Magic Bullet for the Management of Non-Alcoholic Fatty Liver Disease?
Authors: Kim Mi-Young , Choi Sang-Woon
Published: December 2020

Walnut contains many nutrients and bioactive components such as essential fatty acids, polyphenols, fiber, ɤ-tocopherol, folate, minerals, and vegetable protein, and has therefore been regarded as a natural functional food. Walnut-enriched diets have been demonstrated to be useful for heart health, cancer prevention, and metabolic disorders owing to their anti-oxidative and anti-inflammatory properties as well as for the maintenance of a healthy metabolism and immune function. Walnut extracts, either phenolic or lipid, also demonstrated the health effects in animal and cultured cell studies. More recently, the beneficial effects of walnut consumption on non-alcoholic fatty liver disease, which is a hepatic manifestation of obesity, hyperlipidemia, type 2 diabetes mellitus, and metabolic syndrome with substantial hepatic accumulation of triglyceride, have been proposed because walnut and a walnut-containing diet can modulate the etiologic mechanism such as ameliorating systemic and hepatic dyslipidemia, reducing lipotoxicity and inflammation, enhancing immune function, and maintaining gut microbiota balance. Through the extensive literature review we discuss the preventive roles of walnut in the development and progression of non-alcoholic fatty liver disease (NAFLD) and provide mechanistic insights into these effects.
Ibrutinib Could Suppress CA-125 in Ovarian Cancer: A Hypothesis
Authors: Metzler Julian Matthias , Fink Daniel , Imesch Patrick
Published: December 2020

Ibrutinib is a small-molecule inhibitor of Bruton’s tyrosine kinase, an enzyme central in B cell development. It is indicated as a therapy for certain hematological diseases such as chronic lymphocytic leukemia (CLL), but also exerts off-target effects on several receptors and kinases. In this paper, we hypothesize that ibrutinib may suppress the tumor marker CA-125 in ovarian cancer. The hypothesis is based on an observation of CA-125 normalization in a patient with low-grade serous ovarian cancer who received ibrutinib for concurrent CLL. We propose a mechanistic model explaining this possible drug effect as a foundation for further research.
Quality Assessment of Honey Powders Obtained by High- and Low-Temperature Spray Drying
Authors: Jedlińska Aleksandra , Wiktor Artur , Witrowa-Rajchert Dorota , Derewiaka Dorota , Wołosiak Rafał , Matwijczuk Arkadiusz , Niemczynowicz Agnieszka , Samborska Katarzyna
Published: December 2020

The paper aimed to compare the quality of rapeseed and honeydew honey powders, obtained by two methods of spray drying—traditional at a high temperature (inlet air 180 °C) and innovative low-temperature spray drying with the use of dehumidified air as a drying medium (inlet air 75 °C). Total polyphenol content, antioxidant activity, and the content and types of aromatic compounds were determined. In addition, Fourier-transform infrared spectroscopy (FTIR) coupled with chemometrics analyses was done. Powders obtained by the low-temperature spray drying method (with dehumidified air) were characterized by a higher content of polyphenols, antioxidant activity, and aromatic compounds, compared to powders obtained by the traditional method. Honeydew honey compared to rapeseed honey was characterized by a higher content of polyphenols, antioxidant activity, and composition of aromatic compounds. The results proved that the production method had a higher impact on the final properties of powders than the type of honey.
Effect of Burner Operation on the Catalyst Tube Lifetime of a Steam Methane Reformer: A Numerical Study
Authors: Yeh Chun-Lang
Published: December 2020

In this paper, the catalyst tube lifetime of a practical steam methane reformer is analyzed numerically. The effect of burner operating mode on the flow development, hydrogen yield, and catalyst tube lifetime is discussed, with the aim of improving the reformer performance. The results of this study reveal that using the periodic boundary conditions, the temperatures and hydrogen yields obtained are much lower than the experimental values and the pressures are much lower than those using the real model. This results in overestimating the catalyst tube lifetime and underestimating the reformer operation risk. The catalyst tubes in the downstream area have longer lifetimes, while those in the upstream area have shorter lifetimes. Turning the upstream burners off is more efficient to the catalyst tube lifetime, while turning off the central groups of burners is less efficient. The main drawback of turning off burners is the decrease of hydrogen yield.
Novel Epigallocatechin-3-Gallate (EGCG)-Loaded Mesoporous Bioglass Scaffolds for Bone Recruitment Applications
Authors: Ou Shih-Fu , Tsao Yuan-Li , Lin Wei-Chun , Wang Yi-Ting , Wang Liping , Fan Fang-Yu
Published: December 2020

Bioglass-based material has been widely used in the field of biomedical science. In this study, the proper concentration of epigallocatechin-3-gallate (EGCG) for a mesoporous bioglass (MBG) scaffold was determined based on the sponge replication method. The fabrication procedure performed using a foam exchange technique resulted in an interconnected network of pores scaffolds with no cracks. In the minimum bactericidal concentrations of the bacteria assessed, the antibacterial concentration of EGCG against E. coli (200 μg/mL) was higher than that against S. aureus (25 μg/mL). The MBG and EGCG-MBG scaffolds exhibited excellent apatite mineralization and drug release abilities (the highest cumulative drug release from the EGCG-MBG scaffold was 75.37%). Thus, a 200 μg/mL EGCG can prevent cell apoptosis and directly enhance cell proliferation. Hence, a low-dose EGCG-MBG scaffold is another option for bone recruitment material.
The Meaning of the Snake in the Ancient Greek World
Authors: Rodríguez Pérez Diana
Published: December 2020

Despite playing no meaningful practical role in the lives of the ancient Greeks, snakes are ubiquitous in their material culture and literary accounts, in particular in narratives which emphasise their role of guardian animals. This paper will mainly utilise vase paintings as a source of information, with literary references for further elucidation, to explain why the snake had such a prominent role and thus clarify its meaning within the cultural context of Archaic and Classical Greece, with a particular focus on Athens. Previous scholarship has tended to focus on dualistic opposites, such as life/death, nature/culture, and creation/destruction. This paper argues instead that ancient Greeks perceived the existence of a special primordial force living within, emanating from, or symbolised by the snake; a force which is not more—and not less—than pure life, with all its paradoxes and complexities. Thus, the snake reveals itself as an excellent medium for accessing Greek ideas about the divine, anthropomorphism, and ancestry, the relationship between humans, nature and the supernatural, and the negotiation of the inevitable dichotomy of old and new.
Collagen Structure-Function Mapping Informs Applications for Regenerative Medicine
Authors: San Antonio James D. , Jacenko Olena , Fertala Andrzej , Orgel Joseph P.R.O.
Published: December 2020

Type I collagen, the predominant protein of vertebrates, assembles into fibrils that orchestrate the form and function of bone, tendon, skin, and other tissues. Collagen plays roles in hemostasis, wound healing, angiogenesis, and biomineralization, and its dysfunction contributes to fibrosis, atherosclerosis, cancer metastasis, and brittle bone disease. To elucidate the type I collagen structure-function relationship, we constructed a type I collagen fibril interactome, including its functional sites and disease-associated mutations. When projected onto an X-ray diffraction model of the native collagen microfibril, data revealed a matrix interaction domain that assumes structural roles including collagen assembly, crosslinking, proteoglycan (PG) binding, and mineralization, and the cell interaction domain supporting dynamic aspects of collagen biology such as hemostasis, tissue remodeling, and cell adhesion. Our type III collagen interactome corroborates this model. We propose that in quiescent tissues, the fibril projects a structural face; however, tissue injury releases blood into the collagenous stroma, triggering exposure of the fibrils’ cell and ligand binding sites crucial for tissue remodeling and regeneration. Applications of our research include discovery of anti-fibrotic antibodies and elucidating their interactions with collagen, and using insights from our angiogenesis studies and collagen structure-function model to inform the design of super-angiogenic collagens and collagen mimetics.
High Levels of Prebiotic Resistant Starch in Diet Modulate a Specific Pattern of miRNAs Expression Profile Associated to a Better Overall Survival in Pancreatic Cancer
Authors: Trivieri Nadia , Panebianco Concetta , Villani Annacandida , Pracella Riccardo , Latiano Tiziana Pia , Perri Francesco , Binda Elena , Pazienza Valerio
Published: December 2020

Dietary patterns are well known risk factors involved in cancer initiation, progression, and in cancer protection. Previous in vitro and in vivo studies underline the link between a diet rich in resistant starch (RS) and slowing of tumor growth and gene expression in pancreatic cancer xenograft mice. The aim of this study was to investigate the impact of a diet rich in resistant starch on miRNAs and miRNAs-target genes expression profile and on biological processes and pathways, that play a critical role in pancreatic tumors of xenografted mice. miRNA expression profiles on tumor tissues displayed 19 miRNAs as dysregulated in mice fed with RS diet as compared to those fed with control diet and differentially expressed miRNA-target genes were predicted by integrating (our data) with a public human pancreatic cancer gene expression dataset (GSE16515). Functional and pathway enrichment analyses unveiled that miRNAs involved in RS diet are critical regulators of genes that control tumor growth and cell migration and metastasis, inflammatory response, and, as expected, synthesis of carbohydrate and glucose metabolism disorder. Mostly, overall survival analysis with clinical data from TCGA (n = 175) displayed that almost four miRNAs (miRNA-375, miRNA-148a-3p, miRNA-125a-5p, and miRNA-200a-3p) upregulated in tumors from mice fed with RS were a predictor of good prognosis for pancreatic cancer patients. These findings contribute to the understanding of the potential mechanisms through which resistant starch may affect cancer progression, suggesting also a possible integrative approach for enhancing the efficacy of existing cancer treatments.
Histopathological Chromogranin A-Positivity Is Associated with Right-Sided Colorectal Cancers and Worse Prognosis
Authors: Herold Zoltan , Dank Magdolna , Herold Magdolna , Nagy Peter , Rosta Klara , Somogyi Aniko
Published: December 2020

Background: Colorectal cancer (CRC) is known to be affected by paraneoplastic thrombocytosis and chromogranin A-positive neuroendocrine-cell differentiation (CgA+). Their combined effect has never been previously investigated. Methods: A prospective cohort pilot study of 42 CRC patients and 42 age- and sex-matched controls was carried out. Plasma interleukin-6, thrombopoietin, and serum chromogranin A and -B were measured; furthermore, tumor tissue was immunohistochemically stained for CgA+. Results: Twenty-seven and 15 patients were assigned to the chromogranin A-negative (CgA−) and CgA+ groups, respectively. Within the CgA+ group, right-sided tumors were more frequent (18.5% vs. 53.3%), no stage I cancer was found, and patients of this group were in worse general condition. Compared to control subjects, chromogranin A level was higher in the CgA+ group (p = 0.0086), thrombopoietin (p = 0.0040) and chromogranin B (p = 0.0070) in the CgA− group, while interleukin-6 was high in both tumor groups (p ≤ 0.0090). Survival was significantly worse in the CgA+ group (hazard ratio: 5.73; p = 0.0378). Conclusions: Different thrombopoietin levels indicated distinct thrombocytosis types. Within the two CRC groups, serum levels of chromogranins changed in different directions suggesting two well-distinguishable pathophysiologies. Based on these observations we propose a new subtype of CRC, which can be characterized by chromogranin A-positive neuroendocrine-cell differentiation.
A Fenofibrate Diet Prevents Paclitaxel-Induced Peripheral Neuropathy in Mice
Authors: Caillaud Martial , Patel Nipa H. , Toma Wisam , White Alyssa , Thompson Danielle , Mann Jared , Tran Tammy H. , Roberts Jane L. , Poklis Justin L. , Bigbee John W. , Fang Xianjun , Gewirtz David A. , Damaj M. Imad
Published: December 2020

Background: Paclitaxel-induced peripheral neuropathy (PIPN) is a major adverse effect of this chemotherapeutic agent that is used in the treatment of a number of solid malignancies. PIPN leads notably to burning pain, cold and mechanical allodynia. PIPN is thought to be a consequence of alterations of mitochondrial function, hyperexcitability of neurons, nerve fiber loss, oxidative stress and neuroinflammation in dorsal root ganglia (DRG) and spinal cord (SC). Therefore, reducing neuroinflammation could potentially attenuate neuropathy symptoms. Peroxisome proliferator-activated receptor-α (PPAR-α) nuclear receptors that modulate inflammatory responses can be targeted by non-selective agonists, such as fenofibrate, which is used in the treatment of dyslipidemia. Methods: Our studies tested the efficacy of a fenofibrate diet (0.2% and 0.4%) in preventing the development of PIPN. Paclitaxel (8 mg/kg) was administered via 4 intraperitoneal (i.p.) injections in C57BL/6J mice (both male and female). Mechanical and cold hypersensitivity, wheel running activity, sensory nerve action potential (SNAP), sciatic nerve histology, intra-epidermal fibers, as well as the expression of PPAR-α and neuroinflammation were evaluated in DRG and SC. Results: Fenofibrate in the diet partially prevented the development of mechanical hypersensitivity but completely prevented cold hypersensitivity and the decrease in wheel running activity induced by paclitaxel. The reduction in SNAP amplitude induced by paclitaxel was also prevented by fenofibrate. Our results indicate that suppression of paclitaxel-induced pain by fenofibrate involves the regulation of PPAR-α expression through reduction in neuroinflammation. Finally, co-administration of paclitaxel and the active metabolite of fenofibrate (fenofibric acid) did not interfere with the suppression of tumor cell growth or clonogenicity by paclitaxel in ovarian and breast cancer cell lines. Conclusions: Taken together, our results show the therapeutic potential of fenofibrate in the prevention of PIPN development.
Prognostic Impact of Pedicle Clamping during Liver Resection for Colorectal Metastases
Authors: Schiergens Tobias S. , Drefs Moritz , Dörsch Maximilian , Kühn Florian , Albertsmeier Markus , Niess Hanno , Schoenberg Markus B. , Assenmacher Matthias , Küchenhoff Helmut , Thasler Wolfgang E. , Guba Markus O. , Angele Martin K. , Rentsch Markus , Werner Jens , Andrassy Joachim
Published: December 2020

Pedicle clamping (PC) during liver resection for colorectal metastases (CRLM) is used to reduce blood loss and allogeneic blood transfusion (ABT). The effect on long-term oncologic outcomes is still under debate. A retrospective analysis of the impact of PC on ABT-demand regarding overall (OS) and recurrence-free survival (RFS) in 336 patients undergoing curative resection for CRLM was carried out. Survival analysis was performed by both univariate and multivariate methods and propensity-score (PS) matching. PC was employed in 75 patients (22%). No increased postoperative morbidity was monitored. While the overall ABT-rate was comparable (35% vs. 37%, p = 0.786), a reduced demand for more than two ABT-units was observed (p = 0.046). PC-patients had better median OS (78 vs. 47 months, p = 0.005) and RFS (36 vs. 23 months, p = 0.006). Multivariate analysis revealed PC as an independent prognostic factor for OS (HR = 0.60; p = 0.009) and RFS (HR = 0.67; p = 0.017). For PC-patients, 1:2 PS-matching (N = 174) showed no differences in the overall ABT-rate compared to no-PC-patients (35% vs. 40%, p = 0.619), but a trend towards reduced transfusion requirement (>2 ABT-units: 9% vs. 21%, p = 0.052; >4 ABT-units: 2% vs. 11%, p = 0.037) and better survival (OS: 78 vs. 44 months, p = 0.088; RFS: 36 vs. 24 months; p = 0.029). Favorable long-term outcomes and lower rates of increased transfusion demand were observed in patients with PC undergoing resection for CRLM. Further prospective evaluation of potential oncologic benefits of PC in these patients may be meaningful.
Long-Term Dynamic Changes in Cosmetic Outcomes and Patient Satisfaction after Implant-Based Postmastectomy Breast Reconstruction and Contralateral Mastopexy with or without an Ultrapro Mesh Sling Used for the Inner Bra Technique. A Retrospective Correlational Study
Authors: József Zsófia , Újhelyi Mihály , Ping Orsolya , Domján Szilárd , Fülöp Rita , Ivády Gabriella , Tislér Ráhel , Rubovszky Gábor , Mészáros Norbert , Kenessey István , Mátrai Zoltán
Published: December 2020

Immediate implant-based postmastectomy breast reconstruction (IPMBR) with contralateral symmetrization has mostly short-term limited evidence of cosmetic outcomes. Because 84% of early-stage breast cancer patients have overall survival of more than 10 years, reconstructed breast symmetry should provide long-lasting results and acceptable patient satisfaction. Ageing, changes in body weight, and biomechanical changes after IPMBR and symmetrization may contribute to symmetry worsening. This non-interventional single-centre retrospective correlational study presents the clinical and aesthetic results of synthetic ULTRAPRO® mesh inner bra sling symmetrization mastopexy with standard mastopexies. According to the results, a total of 59 patients were enrolled in the mesh group (MG), and 58 patients were enrolled in the non-mesh group (NMG). There were no significant differences in surgical complications (p = 0.521; chi-square). The median sternal notch-to-nipple distance difference was 1 cm in the MG and 3.5 cm in the NMG from the last follow-up, and the median nipple-to-inferior mammary fold distance differences were 0.5 cm and 0.75 cm. The mesh did not hinder the follow-up investigation. In conclusion, ULTRAPRO® mesh sling symmetrization can be successfully used to decrease pseudoptosis and nipple down-migration, offering a safe alternative for long-lasting symmetry and high patient satisfaction.
Neuronal Metabolism and Neuroprotection: Neuroprotective Effect of Fingolimod on Menadione-Induced Mitochondrial Damage
Authors: Gil Antonio , Martín-Montañez Elisa , Valverde Nadia , Lara Estrella , Boraldi Federica , Claros Silvia , Romero-Zerbo Silvana-Yanina , Fernández Oscar , Pavia Jose , Garcia-Fernandez Maria
Published: December 2020

Imbalance in the oxidative status in neurons, along with mitochondrial damage, are common characteristics in some neurodegenerative diseases. The maintenance in energy production is crucial to face and recover from oxidative damage, and the preservation of different sources of energy production is essential to preserve neuronal function. Fingolimod phosphate is a drug with neuroprotective and antioxidant actions, used in the treatment of multiple sclerosis. This work was performed in a model of oxidative damage on neuronal cell cultures exposed to menadione in the presence or absence of fingolimod phosphate. We studied the mitochondrial function, antioxidant enzymes, protein nitrosylation, and several pathways related with glucose metabolism and glycolytic and pentose phosphate in neuronal cells cultures. Our results showed that menadione produces a decrease in mitochondrial function, an imbalance in antioxidant enzymes, and an increase in nitrosylated proteins with a decrease in glycolysis and glucose-6-phosphate dehydrogenase. All these effects were counteracted when fingolimod phosphate was present in the incubation media. These effects were mediated, at least in part, by the interaction of this drug with its specific S1P receptors. These actions would make this drug a potential tool in the treatment of neurodegenerative processes, either to slow progression or alleviate symptoms.
Pathologically Decreased CSF Levels of Synaptic Marker NPTX2 in DLB Are Correlated with Levels of Alpha-Synuclein and VGF
Authors: Boiten Walter , van Steenoven Inger , Xiao Mei-Fang , Worley Paul , Noli Barbara , Cocco Cristina , Ferri Gian-Luca , Lemstra Afina , Teunissen Charlotte
Published: December 2020

Background: Dementia with Lewy bodies (DLB) is a neurodegenerative disease where synaptic loss and reduced synaptic integrity are important neuropathological substrates. Neuronal Pentraxin 2(NPTX2) is a synaptic protein that drives the GABAergic inhibitory circuit. Our aim was to examine if NPTX2 cerebral spinal fluid (CSF) levels in DLB patients were altered and how these levels related to other synaptic protein levels and to cognitive function and decline. Methods: NPTX2, VGF, and α-synuclein levels were determined in CSF of cognitive healthy (n = 27), DLB (n = 48), and AD (n = 20) subjects. Multiple cognitive domains were tested, and data were compared using linear models. Results: Decreased NPTX2 levels were observed in DLB (median = 474) and AD (median = 453) compared to cognitive healthy subjects (median = 773). Strong correlations between NPTX2, VGF, and α-synuclein were observed dependent on diagnosis. Combined, these markers had a high differentiating power between DLB and cognitive healthy subjects (AUC = 0.944). Clinically, NPTX2 levels related to global cognitive function and cognitive decline in the visual spatial domain. Conclusion: NPTX2 CSF levels were reduced in DLB and closely correlated to decreased VGF and α-synuclein CSF levels. CSF NPTX2 levels in DLB related to decreased functioning in the visual spatial domain.
Fabrication of Zinc Oxide Nanoparticles Deposited on (3-Aminopropyl) Triethoxysilane-Treated Silicon Substrates by an Optimized Voltage-Controlled Electrophoretic Deposition and Their Application as Fluorescence-Based Sensors
Authors: Hazzazi Fawwaz , Young Alex , O’Loughlin Christopher , Daniels-Race Theda
Published: December 2020

In this study, a voltage controlled, reproducible, scalable, and cost-effective approach for depositing zinc oxide (ZnO) nanoparticles (NPs), using electrophoretic deposition (EPD) onto p-type silicon (Si) substrates, has been researched and analyzed for its feasibility with respect to electronic device fabrication and fluorescence-based sensors. Our work presents a detailed investigation to evaluate the influence of ZnO morphology, ZnO concentration, and the method of surface treatment applied to the underlying Si substrates, because these pertain to an optimized EPD system. It has been noted that the ZnO NP structures formed directly atop the (3-aminopropyl) triethoxysilane (APTES)-treated Si substrates were more adhesive, thus resulting in a higher yield of NPs over that of comparable depositions on bare silicon. Our observation is that smaller particle sizes of ZnO will increase the energy emission for fluorescence transmission, eliminate several peak emissions, obtain higher fluorescence quantum yield (FQY) efficiency, and require less excitation energy. The results obtained are promising in relation to the integration of EPD in the fabrication of nano biosensors, PV solar cells, nano electronic devices, and thin film transistors (TFTs), where ZnO improves the reliability, affordability, and increased sensitivity needed for the next generation of nanoscale devices and systems.
Forest Resource Management and Its Climate-Change Mitigation Policies in Taiwan
Authors: Tsai Wen-Tien
Published: December 2020

Based on high carbon emissions in recent years (i.e., about 11 metric tons in 2018) per capita in terms of carbon dioxide equivalents, Taiwan has actively development greenhouse gas (GHG) reduction action plans. One of the action plans has been to promote afforestation and reforestation in non-forested lands for carbon sequestration. Thus, this paper aims to address the forest resources in Taiwan by using the latest national survey, reporting on an interactive analysis of forest carbon sequestration, GHG emissions, and climate-change mitigation policies. In this regard, the methodology is based on the official websites of forest resources, GHG emissions, and carbon sequestration from the yearbooks, national statistics, and regulations relevant to the mitigation policies in the forestry sector. It is found that Taiwan’s forest area is estimated to be 2.197 million hectares, which corresponds to a total forest stock volume of about 502.0 million cubic meters. During the period of 1990–2018, the change in total carbon sequestration did not vary much (with the exception of 2009), decreasing from 23.4 million metric tons in 1990 to 21.4 million metric tons in 2018. Compared to the total carbon dioxide emissions (i.e., 102.4 million metric tons in 1990 and 282.8 million metric tons in 2018), the contribution to GHG mitigation in the forestry sector shows a declining trend. However, biomass (i.e., wood) carbon sequestration indicates a slight increase from 20.4 million metric tons in 2010 to 20.7 million metric tons in 2018 due to the afforestation policy. Obviously, regulatory policies, based on the Forestry Act and the Greenhouse Gas Reduction & Management Act in 2015, play a vital role in mitigating GHG emissions in Taiwan. The discussion on the regulations is further addressed to highlight climate-change mitigation policies in Taiwan’s forestry sector.
Comparison between Ischemic and Hemorrhagic Strokes in Functional Outcome at Discharge from an Intensive Rehabilitation Hospital
Authors: Salvadori Emilia , Papi Gioele , Insalata Greta , Rinnoci Valentina , Donnini Ida , Martini Monica , Falsini Catuscia , Hakiki Bahia , Romoli Annamaria , Barbato Carmen , Polcaro Paola , Casamorata Francesca , Macchi Claudio , Cecchi Francesca , Poggesi Anna
Published: December 2020

Comparison studies on recovery outcomes in ischemic (IS) and hemorrhagic strokes (HS) have yielded mixed results. In this retrospective observational study of consecutive IS and HS patients, we aimed at evaluating functional outcomes at discharge from an intensive rehabilitation hospital, comparing IS vs. HS, analyzing possible predictors. Modified Rankin Scale (mRS) at discharge was the main outcome. Out of the 229 patients included (mean age 72.9 ± 13.9 years, 48% males), 81 had HS (35%). Compared with IS (n = 148), HS patients were significantly younger (75 ± 12.5 vs. 68.8 ± 15.4 years, p = 0.002), required longer hospitalizations both in acute (23.9 ± 36.7 vs. 35.2 ± 29.9 days, p = 0.019) and rehabilitation hospitals (41.5 ± 31.8 vs. 77.2 ± 51.6 days, p = 0.001), and had more severe initial clinical deficit (mean number of neurological impairments: 2.0 ± 1.1 vs. 2.6 ± 1.4, p = 0.001) and mRS scores at admission (p = 0.046). At discharge, functional status change, expressed as mRS, was not significantly different between IS and HS (F = 0.01, p = 0.902), nor was the discharge destination (p = 0.428). Age and clinical severity were predictors of functional outcome in both stroke types. On admission in an intensive rehabilitation hospital, HS patients presented a worse functional and clinical status compared to IS. Despite this initial gap, the two stroke types showed an overlapped trajectory of functional recovery, with age and initial stroke severity as the main prognostic factors.
Boltzmann Machines as Generalized Hopfield Networks: A Review of Recent Results and Outlooks
Authors: Marullo Chiara , Agliari Elena
Published: December 2020

The Hopfield model and the Boltzmann machine are among the most popular examples of neural networks. The latter, widely used for classification and feature detection, is able to efficiently learn a generative model from observed data and constitutes the benchmark for statistical learning. The former, designed to mimic the retrieval phase of an artificial associative memory lays in between two paradigmatic statistical mechanics models, namely the Curie-Weiss and the Sherrington-Kirkpatrick, which are recovered as the limiting cases of, respectively, one and many stored memories. Interestingly, the Boltzmann machine and the Hopfield network, if considered to be two cognitive processes (learning and information retrieval), are nothing more than two sides of the same coin. In fact, it is possible to exactly map the one into the other. We will inspect such an equivalence retracing the most representative steps of the research in this field.
Estimating Needle and Shoot Inclination Angle Distributions and Projection Functions in Five Larix principis-rupprechtii Plots via Leveled Digital Camera Photography
Authors: Zou Jie , Zhong Peihong , Hou Wei , Zuo Yong , Leng Peng
Published: December 2020

The leaf inclination angle distribution function is a key determinant that influences radiation penetration through forest canopies. In this study, the needle and shoot inclination angle distributions of five contrasting Larix principis-rupprechtii plots were obtained via the frequently used leveled digital camera photography method. We also developed a quasi-automatic method to derive the needle inclination angles based on photographs obtained using the leveled digital camera photography method and further verified using manual measurements. Then, the variations of shoot and needle inclination angle distributions due to height levels, plots, and observation years were investigated. The results showed that the developed quasi-automatic method is effective in deriving needle inclination angles. The shoot and needle inclination angle distributions at the whole-canopy scale tended to be planophile and exhibited minor variations with plots and observation years. The small variations in the needle inclination angle distributions with height level in the five plots might be caused by contrasting light conditions at different height levels. The whole-canopy and height level needle projection functions also tended to be planophile, and minor needle projection function variations with plots and observation years were observed. We attempted to derive the shoot projection functions of the five plots by using a simple and applicable method and further evaluated the performance of the new method.
Root-Plate Characteristics of Common Aspen in Hemiboreal Forests of Latvia: A Case Study
Authors: Samariks Valters , Brizga Dace , Rūba Jeļena , Seipulis Andris , Jansons Āris
Published: December 2020

Climate change will cause winds to strengthen and storms to become more frequent in Northern Europe. Windstorms reduce the financial value of forests by bending, breaking, or uprooting trees, and wind-thrown trees cause additional economic losses. The resistance of trees to wind damage depends on tree species, tree- and stand-scale parameters, and root-soil plate characteristics such as root-plate size, weight, and rooting depth. The root-soil plate is a complex structure whose mechanical strength is dependent on root-plate width and depth, as the root system provides root attachment with soil and structural support. In Latvia, the common aspen (Populus tremula L.) root system has been studied to develop a belowground biomass model, because information about root system characteristics in relation to tree wind resistance is scarce. The aim of this study was to assess the root-plate dimensions of common aspen stands on fertile mineral soil (luvisol). Study material was collected in the central region of Latvia, where pure mature (41–60 years old) common aspen stands were randomly selected, and dominant trees within the stand were chosen. In total, ten sample trees from ten stands were uprooted. The diameter at breast height (DBH) and tree height (H) were measured for each sample tree, and their roots were excavated, divided into groups, washed, measured, and weighed. The highest naturally moist biomass values were observed for coarse roots, and fine root biomass was significantly lower compared to other root groups. All root group biomass values had a strong correlation with the tree DBH. The obtained results show that there is a close, negative relationship between the relative distance from the stem and the relative root-plate depth distribution.
Protective Effect of Prunus mume Fermented with Mixed Lactic Acid Bacteria in Dextran Sodium Sulfate-Induced Colitis
Authors: Kim Jeong-Ho , Won Yeong-Seon , Cho Hyun-Dong , Hong Seong-Min , Moon Kwang-Deog , Seo Kwon-Il
Published: December 2020

The fruit of Prunus mume (PM) is widely cultivated in East Asia, and it has been used as a folk medication for gastrointestinal disorders, e.g., diarrhea, stomach ache and ulceration. In this study, the pectinase-treated PM juice (PJ) was fermented with Lactobacillus strains containing fundamental organic acids and free amino acids. The PJ fermented with Lactobacillus plantarum and L. casei (FP) was investigated for its protective effect in dextran sodium sulfate (DSS)-induced colitis mice model. The administration of FP reduced lipid peroxidation and histopathological colitis symptoms, e.g., shortening of the colon length, depletion of mucin, epithelial injury and ulceration, in colonic tissues. The FP-supplemented group showed the alleviation of pro-inflammatory cytokines. Compared with the DSS control group, the supplementation of FP significantly reduced the levels of serum interferon-γ (IFN-γ), interleukin (IL)-1β, IL-6, IL-12 and IL-17 as well as colonic tumor necrosis factor-α, IFN-γ, IL-12 and IL-17. Furthermore, the DSS-induced TUNEL-positive area was significantly reduced by the FP supplementation. These results show that the supplementation of FP fermented with mixed lactic acid bacteria, L. plantarum and L. casei, elucidated the protective effect in DSS-induced colitis mice. Hence, this study suggests that FP can be utilized as a natural therapeutic agent for colitis and intestinal inflammation.
Nutrition in Disguise: Effects of Food Neophobia, Healthy Eating Interests and Provision of Health Information on Liking and Perceptions of Nutrient-Dense Foods in Older Adults
Authors: Romaniw Olivia C. , Rajpal Ritika , Duncan Alison M. , Keller Heather H. , Duizer Lisa M.
Published: December 2020

Older adults (60+ years) are at higher risk of malnutrition. Improving the nutrient-density of their diets is important but presents challenges due to the introduction of new ingredients, liking implications and heterogeneity of older consumers. Ten nutrient-enhanced foods were evaluated for liking (9-point hedonic scale) and sensory perception (check-all-that-apply) by 71 older adults. Three foods were re-evaluated after participants were provided with information about their healthy ingredients and benefits. Participants were also segmented based on their degrees of food neophobia and interests in healthy eating, using questionnaires. The results showed that eight foods had adequate sensory appeal (overall hedonic score of ≥6) to be pursued for residential care menus. Segmentation based on food neophobia and healthy eating interests did not yield any meaningful differences between groups. The effect of health information on liking for the overall sample and subgroups was product-specific: liking scores only increased for the raspberry banana smoothie in the overall test population and higher healthy eating interest subgroup. Health information may lead to the experience of more positive attributes in some foods. Overall, eight foods that were tested could be accepted by a wide range of consumers and providing them with health information may further improve acceptance.