bluuebunny/crossref_metadata_2025 huggingface dataset:
The Effectiveness and Safety of Immune Checkpoint Inhibitors in Non-Small Cell Lung Cancer Patients With Stage III/IV: A Multicenter Study
Authors: Hsu Jason C. , Nguyen Phung-Anh , Chen Yen-Tzu , Yang Szu-Chun , Lin Chien-Chung , Yang Yi-Hsin , Lin Yu-Chao , Hsia Te-Chun , Hsieh Hsing-Chun , Wu Jia-Syuan , Chang Chi-Pei , Feng Yin-Hsun , Lin Peng-Chan , Hsu Ping-Chih , Tzeng Huey-En , Chien Shu-Chen , Chang Wei-Chiao , Chang Chih-Cheng , Yang Hsuan-Chia , Lee Chueh Ming , Lu Christine Y.
Published: July 2021

Immune checkpoint inhibitors (ICIs) have been approved to treat patients with various cancer types, including lung cancer, in many countries. This study aims to investigate the effectiveness and safety of ICIs under different treatment conditions of non-small cell lung cancer patients. A population-based retrospective cohort study was conducted using the electronic health records of three medical centers in Taiwan. From January 01, 2016, to November 30, 2018, a total of 91 ICIs and 300 traditional chemotherapy users who had undergone stage III and IV lung cancer treatment were included in the study. We performed the randomized matched pair design by selecting a Chemotherapy subject for each ICI patient in the sample population. All subjects were monitored from the date of taking ICIs or chemotherapy drugs until the event of death, loss to follow-up, or were occurred with any defined adverse events. Kaplan-Meier estimators and cox proportional hazard regression models were used to compute the overall survival, efficacy, and safety of the ICIs group. The median overall survival (OS) in the ICI and Chemo groups after matching was 11.2 months and 10.5 months, respectively. However, the results showed no significant OS differences between ICIs and chemo groups for both before and after matching (HR,1.30; 95%CI, 0.68-2.46; p=0.428 before matching and HR,0.96; 95CI%, 0.64-1.44; p=0.838 after matching). We observed that with the higher amount of PD-L1, the length of the patients’ overall survival was (positive vs. negative PD-L1, HR,0.21; 95%CI, 0.05-0.80; p=0.022). The incidences of serious adverse drug events above grade 3 in the ICIs and traditional chemo groups were 12.7% and 21.5%, respectively. We also found that the number of AEs was less in ICIs than in the Chemo group, and the AEs that occurred after treatments were observed earlier in the ICIs compared to the Chemo group. ICIs drugs were observed to be safer than traditional chemotherapy as they had a lower risk of serious adverse drug events. It is necessary to pay attention to immune-related side effects and provide appropriate treatment. Furthermore, the patient’s physical status and PD-L1 test can be used to evaluate the clinical effectiveness of ICIs.
Integrated Analysis of the Clinical and Molecular Characteristics of IDH Wild-Type Gliomas in the Chinese Glioma Genome Atlas
Authors: Wang Peng , Liu Yanwei , Zhi Lin , Qiu Xiaoguang
Published: July 2021

Purpose

Current studies and guidelines suggest that the biobehavior of IDH-wild type (IDH-wt) lower-grade glioma (LGG, WHO II-III) is similar to IDH-wt glioblastoma (GBM). However, differences in their clinical and molecular characteristics have not been reported. This study aimed to analyze the clinical and genetic information of gliomas with IDH-wt.

Methods

389 patients with IDH-wt were enrolled in the study (LGG=165, GBM=224), and their clinical and genetic information was collected from the Chinese Glioma Genome Atlas (CGGA). We conducted an analysis of this information between the two groups of patients and drew conclusions thereof.

Results

The median age of the LGG patients was 42 (18–74) years, whereas that of the GBM patients was 51 (18–79) years (P < 0.010). GBM patients were more likely to undergo total resection (P = 0.018) and had fewer epileptic seizure symptoms (P < 0.001). The median overall survival (OS) was 55 months for the LGG patients and only 14.83 months for the GBM patients (P < 0.01). The median progression-free survival (PFS) was 44 months for the LGG patients and only 9.767 months for the GBM patients (P < 0.001). GBM patients were more prone to PETN mutations (P = 0.010). Transcriptome analysis showed that the differentially expressed genes in LGG patients were mainly enriched in metabolic pathways and pathways in cancer and in the function of signal transduction and positive regulation of GTPase activity, whereas in GBM patients, they were mainly enriched in the PI3K-Akt signaling pathway and in the functions of apoptotic process and oxidation-reduction process.

Conclusions

Our data indicate that these two groups of patients should be re-evaluated and treated differently, despite both having IDH wild type.
α-Mangostin Alleviated Inflammation in Rats With Adjuvant-Induced Arthritis by Disrupting Adipocytes-Mediated Metabolism-Immune Feedback
Authors: Hu Ying-Hao , Han Jun , Wang Lin , Shi Chao , Li Yan , Olatunji Opeyemi Joshua , Wang Xiu , Zuo Jian
Published: July 2021

A previously identified anti-rheumatic compound α-mangostin (MAN) possesses notable metabolism regulatory properties. In this study, we investigated the immune implication of MAN-altered fat metabolism on adjuvant-induced arthritis (AIA) in rats. Seven days after AIA induction, the rats received oral treatment of MAN at 50 mg/kg/day for 30 days. Metabolic indicators and basic clinical parameters were evaluated using samples collected on day 20 and 38 since immunization. Expression of nicotinamide phosphoribosyltransferase (NAMPT), sirtuin 1 (SIRT1), peroxisome proliferator activated receptor gamma (PPAR-γ), stearoyl-coa desaturase 1 (SCD-1), toll like receptor 4 (TLR4), prostaglandin-endoperoxide synthase 2 (COX-2), (p)-JNK, (p)-p65 and IL-1β were investigated by either RT-qPCR or immunobloting methods. In in vitro experiments, we treated (pre)-adipocytes with monocytes/macrophages and MAN, and investigated the changes of macrophages brought by pre-adipocytes co-culture. Generally, MAN restored the impaired fat anabolism in AIA rats, indicated by increased fat reservoir, leptin and adiponectin secretion, and PPAR-γ and SCD-1 expression. Meanwhile, it decreased circulating IL-1β and IL-6 levels, restored serological lipid profile changes, and relieved oxidative stresses, demonstrating potent therapeutic effects on AIA. AIA rats-derived monocytes inhibited mRNA PPAR-γ and SCD-1 expression in pre-adipocytes. Contrarily, MAN facilitated adipocyte differentiation in vitro, and increased free fatty acids production. It also significantly increased PPAR-γ and SCD-1 expression, which can be abrogated by PPAR-γ inhibitor T0070907. Similarly, lipopolysaccharide-primed macrophages inhibited PPAR-γ expression in the co-cultured pre-adipocytes, which was reversed by MAN. In the same co-culture system, lipopolysaccharide-induced inflammation was amplified by the co-existence of pre-adipocytes. More secretion of IL-1β and IL-6 and higher levels expression of COX-2, p-JNK, p-p65 and TLR4 were observed in lipopolysaccharide-treated macrophages when co-cultured by pre-adipocytes. The intensified inflammatory situation was eased by MAN. The treatment with pre-adipocytes culture medium achieved similar effects. Medium from lipopolysaccharide-treated adipocytes promoted IL-1β, IL-6 and MCP-1 production in separately cultured macrophages, and COX-2, p-JNK, p-p65 and TLR4 expression were increased at the meantime. MAN treatment on pre-adipocytes impaired these changes. It suggests that fat anabolism in AIA rats was deficient due to increased energy expenditure caused by inflammatory conditions. MAN restored fat metabolism homeostasis by up-regulating PPAR-γ, and reshaped secretion profile of adipocytes.
Caveolin-1 Alleviates Acetaminophen-Induced Fat Accumulation in Non-Alcoholic Fatty Liver Disease by Enhancing Hepatic Antioxidant Ability via Activating AMPK Pathway
Authors: Wang Jiarong , Jiang Wei , Xin Jiao , Xue Weiju , Shi Congjian , Wen Jiagen , Huang Yan , Hu Chengmu
Published: July 2021

Non-alcoholic fatty liver disease (NAFLD) is an independent risk factor for acute liver injury caused by overuse of acetaminophen (APAP). Caveolin-1 (CAV1), a regulator of hepatic energy metabolism and oxidative stress, was found to have a protective effect against NAFLD in our previous study. However, it remains unclear whether CAV1 has a protective effect against APAP-induced hepatotoxicity in NAFLD. The aim of this study was to determine whether CAV1 inhibits oxidative stress through the AMPK/Nrf2/HO-1 pathway to protect the liver from fat accumulation exacerbated by APAP in NAFLD. In this study, seven-week-old C57BL/6 male mice (18–20 g) were raised under similar conditions for in vivo experiment. In vitro, L02 cells were treated with A/O (alcohol and oleic acid mixture) for 48 h, and APAP was added at 24 h for further incubation. The results showed that the protein expression of the AMPK/Nrf2 pathway was enhanced after CAV1 upregulation. The effects of CAV1 on fat accumulation, ROS, and the AMPK/Nrf2 anti-oxidative pathway were reduced after the application of CAV1-siRNA. Finally, treatment with compound C (an AMPK inhibitor) prevented CAV1 plasmid-mediated alleviation of oxidative stress and fat accumulation and reduced the protein level of Nrf2 in the nucleus, demonstrating that the AMPK/Nrf2/HO-1 pathway was involved in the protective effect of CAV1. These results indicate that CAV1 exerted a protective effect against APAP-aggravated lipid deposition and hepatic injury in NAFLD by inhibiting oxidative stress. Therefore, the upregulation of CAV1 might have clinical benefits in reducing APAP-aggravated hepatotoxicity in NAFLD.
Self-Organized Criticality in the Brain
Authors: Plenz Dietmar , Ribeiro Tiago L. , Miller Stephanie R. , Kells Patrick A. , Vakili Ali , Capek Elliott L.
Published: July 2021

Self-organized criticality (SOC) refers to the ability of complex systems to evolve toward a second-order phase transition at which interactions between system components lead to scale-invariant events that are beneficial for system performance. For the last two decades, considerable experimental evidence has accumulated that the mammalian cortex with its diversity in cell types, interconnectivity, and plasticity might exhibit SOC. Here, we review the experimental findings of isolated, layered cortex preparations to self-organize toward four dynamical motifs presently identified in the intact cortex in vivo: up-states, oscillations, neuronal avalanches, and coherence potentials. During up-states, the synchronization observed for nested theta/gamma oscillations embeds scale-invariant neuronal avalanches, which can be identified by robust power law scaling in avalanche sizes with a slope of −3/2 and a critical branching parameter of 1. This precise dynamical coordination, tracked in the negative transients of the local field potential (nLFP) and spiking activity of pyramidal neurons using two-photon imaging, emerges autonomously in superficial layers of organotypic cortex cultures and acute cortex slices, is homeostatically regulated, exhibits separation of time scales, and reveals unique size vs. quiet time dependencies. A subclass of avalanches, the coherence potentials, exhibits precise maintenance of the time course in propagated local synchrony. Avalanches emerge in superficial layers of the cortex under conditions of strong external drive. The balance of excitation and inhibition (E/I), as well as neuromodulators such as dopamine, establishes powerful control parameters for avalanche dynamics. This rich dynamical repertoire is not observed in dissociated cortex cultures, which lack the differentiation into cortical layers and exhibit a dynamical phenotype expected for a first-order phase transition. The precise interactions between up-states, nested oscillations, and avalanches in superficial layers of the cortex provide compelling evidence for SOC in the brain.
Renal Inflammation and Innate Immune Activation Underlie the Transition From Gentamicin-Induced Acute Kidney Injury to Renal Fibrosis
Authors: Albino Amanda Helen , Zambom Fernanda Florencia Fregnan , Foresto-Neto Orestes , Oliveira Karin Carneiro , Ávila Victor Ferreira , Arias Simone Costa Alarcon , Seguro Antonio Carlos , Malheiros Denise Maria Avancini Costa , Camara Niels Olsen Saraiva , Fujihara Clarice Kazue , Zatz Roberto
Published: July 2021

Subjects recovering from acute kidney injury (AKI) are at risk of developing chronic kidney disease (CKD). The mechanisms underlying this transition are unclear and may involve sustained activation of renal innate immunity, with resulting renal inflammation and fibrosis. We investigated whether the NF-κB system and/or the NLRP3 inflammasome pathway remain activated after the resolution of AKI induced by gentamicin (GT) treatment, thus favoring the development of CKD. Male Munich-Wistar rats received daily subcutaneous injections of GT, 80 mg/kg, for 9 days. Control rats received vehicle only (NC). Rats were studied at 1, 30, and 180 days after GT treatment was ceased. On Day 1, glomerular ischemia (ISCH), tubular necrosis, albuminuria, creatinine retention, and tubular dysfunction were noted, in association with prominent renal infiltration by macrophages and myofibroblasts, along with increased renal abundance of TLR4, IL-6, and IL1β. Regression of functional and structural changes occurred on Day 30. However, the renal content of IL-1β was still elevated at this time, while the local renin-angiotensin system remained activated, and interstitial fibrosis became evident. On Day 180, recurring albuminuria and mild glomerulosclerosis were seen, along with ISCH and unabated interstitial fibrosis, whereas macrophage infiltration was still evident. GT-induced AKI activates innate immunity and promotes renal inflammation. Persistence of these abnormalities provides a plausible explanation for the transition of AKI to CKD observed in a growing number of patients.
A Four-MicroRNA Panel in Peripheral Blood Identified as an Early Biomarker to Diagnose Acute Myocardial Infarction
Authors: Chen Liang , Bai Jie , Liu Jun , Lu Huihe , Zheng Koulong
Published: July 2021

Objective: This study aimed to evaluate suitable circulating microRNAs (miRNAs) as diagnostic biomarkers of acute myocardial infarction (AMI).

Methods: Patients with AMI were enrolled as study participants. All patients with AMI coming from the Second Affiliated Hospital of Nantong University between October 1, 2017 and May 31, 2019 were screened. At the same time, 80 patients with coronary angiographic stenosis <50% during the same period were selected as the control group. Peripheral blood samples were collected at different time points (0, 6, 12, and 24 h after disease onset) to detect the expression of a previously identified promising four-microRNA panel. The expression levels of miRNAs were tested by real-time polymerase chain reaction (RT-PCR), and the receiver operating characteristic curve (ROC) was used to analyze the diagnostic value of circulating miRNAs.

Results: Based on the inclusion and exclusion criteria, 80 patients with AMI and 80 controls were enrolled in this study. The expression of circulating miR-1291, miR-217, miR-455-3p, and miR-566 was significantly downregulated in patients with AMI compared with controls. The area under the ROC curve (AUC) of circulating miR-1291, miR-217, miR-455-3p, and miR-566 were 0.82, 0.79, 0.82, and 0.83, respectively. The AUC of these four miRNAs was 0.87 with 83% sensitivity and 87% specificity. The expression peaks of these four miRNAs occurred earlier than those of cardiac troponin I (cTnI) and creatine kinase-MB (CK-MB). Furthermore, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that the targets of these four miRNAs were significantly enriched in several signaling pathways associated with AMI progression.

Conclusion: Circulating miR-1291, miR-217, miR-455-3p, and miR-566 expression levels were significantly lower in patients with AMI; and combined, this panel of four miRNAs acted as a novel and potential early diagnostic biomarker of AMI.
Microbial Identification, High-Resolution Microscopy and Spectrometry of the Rhizosphere in Its Native Spatial Context
Authors: Bandara Chaturanga D. , Schmidt Matthias , Davoudpour Yalda , Stryhanyuk Hryhoriy , Richnow Hans H. , Musat Niculina
Published: July 2021

During the past decades, several stand-alone and combinatorial methods have been developed to investigate the chemistry (i.e., mapping of elemental, isotopic, and molecular composition) and the role of microbes in soil and rhizosphere. However, none of these approaches are currently applicable to characterize soil-root-microbe interactions simultaneously in their spatial arrangement. Here we present a novel approach that allows for simultaneous microbial identification and chemical analysis of the rhizosphere at micro− to nano-meter spatial resolution. Our approach includes (i) a resin embedding and sectioning method suitable for simultaneous correlative characterization of Zea mays rhizosphere, (ii) an analytical work flow that allows up to six instruments/techniques to be used correlatively, and (iii) data and image correlation. Hydrophilic, immunohistochemistry compatible, low viscosity LR white resin was used to embed the rhizosphere sample. We employed waterjet cutting and avoided polishing the surface to prevent smearing of the sample surface at nanoscale. The quality of embedding was analyzed by Helium Ion Microscopy (HIM). Bacteria in the embedded soil were identified by Catalyzed Reporter Deposition-Fluorescence in situ Hybridization (CARD-FISH) to avoid interferences from high levels of autofluorescence emitted by soil particles and organic matter. Chemical mapping of the rhizosphere was done by Scanning Electron Microscopy (SEM) with Energy-dispersive X-ray analysis (SEM-EDX), Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS), nano-focused Secondary Ion mass Spectrometry (nanoSIMS), and confocal Raman spectroscopy (μ-Raman). High-resolution correlative characterization by six different techniques followed by image registration shows that this method can meet the demanding requirements of multiple characterization techniques to identify spatial organization of bacteria and chemically map the rhizosphere. Finally, we presented individual and correlative workflows for imaging and image registration to analyze data. We hope this method will be a platform to combine various 2D analytics for an improved understanding of the rhizosphere processes and their ecological significance.
Looking Through the COVID-19 Window of Opportunity: Future Scenarios Arising From the COVID-19 Pandemic Across Five Case Study Sites
Authors: Richter Isabell , Avillanosa Arlene , Cheung Victoria , Goh Hong Ching , Johari Sofia , Kay Susan , Maharja Carya , Nguyễn Thu Hà , Pahl Sabine , Sugardjito Jito , Sumeldan Joel , van Nguyen Quyen , Vu Hien Thuc , Wan Mohamad Ariffin Wan Nur Syazana , Austen Melanie C.
Published: July 2021

The COVID-19 pandemic has caused (and continues to cause) severe disruption in global and local economies and has forced countries, societies, and individuals to adapt quickly to the unprecedented and unpredictable situations. Despite the obvious negative consequences of the pandemic, many have called for efforts to identify transformative opportunities for sustainable development throughout this disorderly time. In the present paper, we explore such potential opportunities in the context of an interdisciplinary, international research project, which is focusing on sustainable marine management in biosphere reserves and marine parks in Southeast Asia. During a virtual workshop conducted as part of the GCRF (Government’s Global Challenges Research Fund) Blue Communities Project, future scenarios were developed depicting the potential effects of the COVID-19 pandemic on five case study sites. All of these sites are in areas of internationally recognized outstanding ecological value (Taka Bonerate Kepulauan-Selayar Biosphere Reserve, Indonesia; Tun Mustapha Park, Sabah, Malaysia; Palawan Biosphere Reserve, Philippines; North Devon Biosphere Reserve, United Kingdom; Cu Lao Cham-Hoi An Biosphere Reserve, Vietnam). At the macro-level, economies, governance structures, and societal norms are undergoing big changes. At the micro-level, the livelihoods, lifestyles, and backyards of local residents have to adapt. Collaboratively, we explored how COVID-19 posed challenges in our five case study sites, but we also focused on the potential COVID-19-related windows of opportunity for future sustainable development. Opportunities could be identified in all three pillars of sustainable development: the environment, the society, and the economy. Although remarkable similarities can be found across all five sites, we conclude that there cannot be a “one-size-fits-all” solution to turn the tide toward achieving sustainable development. Just as before the pandemic, sustainable development starts with engaging with and understanding local environments, challenges, and situations; building on local knowledge; and developing tailor-made solutions for the communities in situ.
It’s a Challenge, Not a Threat: Lecturers’ Satisfaction During the Covid-19 Summer Semester of 2020
Authors: Feldhammer-Kahr Martina , Tulis Maria , Leen-Thomele Eline , Dreisiebner Stefan , Macher Daniel , Arendasy Martin , Paechter Manuela
Published: July 2021

The summer semester had just begun at Austrian and German universities when Covid-19 was declared a global pandemic by the World Health Organization. Thus, in March 2020, all universities closed their campuses, switching to distance learning within the span of about a single day. How did lecturers handle the situation? Were they still able to turn the situation into a positive one? What were the main obstacles with this difficult situation, and where there conditions which helped them to overcome the new challenges? These are research questions of the present survey with a sample of 1,152 lecturers at universities in Austria and Germany. The survey focuses on the lecturers’ appraisals of the novel situation as challenging or threatful. These appraisals are important for approaching a situation or shying away from it. However, how well a person adjusts to a novel situation is also influenced by personal and environmental resources which help to overcome the situation. The present survey focused on four possible sources of influence: internal assessments of the situation determining it to be threatening and/or challenging, personal resources, attitudes, and support by the organization. It was investigated to which degree these sources of influence could contribute to the lecturers’ satisfaction (or dissatisfaction) with their teaching processes. A multiple regression with three criterion variables describing university lecturers’ perceived satisfaction with distance teaching was carried out. Predictor variables were the lecturers’ appraisals of challenge and threat, perceived support by the university and sense of belonging to the university, temporal resources, proficiency in using digital technologies, length of teaching experience, and gender. Lecturers were mostly satisfied with their teaching activities. Together with the perception of a low threat potential, challenge appraisals contributed strongest to satisfaction. In comparison, assessments of actual personal resources, skills in the use of digital technologies, teaching experience, and temporal resources were important but contributed less to satisfaction than challenge appraisals. It seems that lecturers were only able to use these resources when the technological resources were available and when the lecturers were confident in their technical abilities.
Predicting Contribution in High Achieving Black and Latinx Youth: The Role of Critical Reflection, Hope, and Mentoring
Authors: Bowers Edmond P. , Bolding Candice W. , Rapa Luke J. , Sandoval Alexandra M.
Published: July 2021

Contemporary approaches to adolescent development are framed by positive youth development models. A key outcome of these models is that healthy and positively developing youth are more likely to contribute to their family, schools, and communities. However, little work on contribution and its antecedents has been conducted with youth of color. As high achieving youth of color often become leaders in their communities, it is important to consider malleable predictors of contribution within this population. Therefore, through a cross-sectional design, we examined the relations between youth critical reflection, hopeful future expectations, and mentoring relationship quality and youth contribution in a sample of 177 youth of color (60% Black, 40% Latinx) attending an afterschool college preparation program at six sites around the U.S. Results indicated that youth critical reflection, hopeful future expectations, and mentoring relationship quality significantly predicted contribution. Exploratory analyses suggested that these relations were significant for Black youth but not Latinx youth. Implications of these findings for future scholarship are discussed.
Entrepreneurial Intention and Perceived Social Support From Academics-Scientists at Chilean Universities
Authors: Acuña-Duran Eduardo , Pradenas-Wilson Daniela , Oyanedel Juan Carlos , Jalon-Gardella Roberto
Published: July 2021

Within Ajzen's Planned Behavior Theory framework, this article tests a model to estimate the predictors of entrepreneurial intention in academic scientists working in Chile. We adapted into Spanish the entrepreneurship intention questionnaire. We tested the entrepreneurship intention model on a sample of 1,027 scientists leading research projects funded by the Chilean Scientific and Technological Development Fund (FONDECYT), the country's primary scientific research grant. The results show strong empirical support for the entrepreneurship intention model proposed while highlighting some critical issues specific to entrepreneurial intention in scientists. In particular, we found an indirect effect of perceived subjective social support on entrepreneurial intention, which is mediated by entrepreneurial attitude and perceived behavioral control toward entrepreneurship. These results suggest that policies orientated toward promoting academic entrepreneurship should include developing a healthy social environment toward it, meaning that entrepreneurial intention is not only an individual but an organizational challenge. These policies should analyze the social norms guiding the scientists' reference groups to increase their effectiveness.
On Lies and Hard Truths
Authors: Behnk Sascha , Reuben Ernesto
Published: July 2021

We run an experimental study using sender-receiver games to evaluate how senders' willingness to lie to others compares to their willingness to tell hard truths, i.e., promote an outcome that the sender knows is unfair to the receiver without explicitly lying. Unlike in previous work on lying when it has consequences, we do not find that antisocial behavior is less frequent when it involves lying than when it does not. In fact, we find the opposite result in the setting where there is social contact between senders and receivers, and receivers have enough information to judge whether they have been treated unfairly. In this setting, we find that senders prefer to hide behind a lie and implement the antisocial outcome by being dishonest rather than by telling the truth. These results are consistent with social image costs depending on the social proximity between senders and receivers, especially when receivers can judge the kindness of the senders' actions.
“Antibiotics Can Work as a Contraceptive:” Contraceptive Knowledge and Use Among University Students in Calabar, Nigeria
Authors: Asaolu Ibitola , Kram Nidal , Ajala Christopher , Aquaisua Ememobong , Asaolu Akinsola , Kato-Lagumbay Kylie , Abuh Alice , Bernand Moses , Ehiri John
Published: July 2021

Introduction: Many sexually active youths who wish to delay pregnancy are not using any form of modern contraceptives. In sub-Saharan Africa, less than 1 in 5 sexually active youth do not use contraceptives. In Nigeria, 48.4% of all sexually active unmarried women have an unmet need for contraception. Although the literature is replete with information on structural barriers to modern contraceptives, there is limited scholarship on contextual factors that may inhibit modern contraceptive use among Nigerian youth. This study uses a qualitative research approach to assess knowledge and use of natural, modern, and folkloric contraceptive methods among a sample of university students in Calabar, Nigeria.

Methods: This study used data from focus group discussions among women and men in university halls of residence, all of whom were unmarried. Also, three male pharmacists and three female community health workers were interviewed. All focus group discussions and interviews took place in August 2017 and were conducted in Calabar Metropolis, Cross River State, Nigeria. The audio recordings were transcribed into detailed summaries of the interviews and focus group discussions. All data analysis was completed using Atlas.Ti (version 8).

Results: University men and women have limited knowledge of and application of natural and modern contraception. Participants listed folkloric methods of contraceptives, including repurposing pharmaceuticals (e.g., antibiotics, quinine, and Andrews Livers Salt-a laxative) as contraceptive agents. Respondents also discussed the use of non-pharmaceuticals such as water, salt solution, and squatting after intercourse as contraceptives. Generally, university students defaulted to withdrawal, calendar method, and emergency contraceptives as preferred methods of contraception. Lastly, condoms were used among participants in causal sexual encounters. In dating relationships, however, both male and female students cited their partners' hesitancy to condom use as such act could indicate distrust.

Conclusion: Awareness and use of modern contraceptives are limited among university students in Calabar, Nigeria. The use of folkloric contraceptives could lead to bodily harm and unintended pregnancy. Further research is needed to elucidate factors that promote use of folkloric methods of contraceptives. Integration of contraceptive awareness into health promotion services for young people may help to dispel myths about folkloric methods of contraceptives while promoting awareness and use of safe and effective contraception.
A Novel Lithium Foil Cosmic-Ray Neutron Detector for Measuring Field-Scale Soil Moisture
Authors: Patrignani Andres , Ochsner Tyson E. , Montag Benjamin , Bellinger Steven
Published: July 2021

During the past decade, cosmic-ray neutron sensing technology has enabled researchers to reveal soil moisture spatial patterns and to estimate landscape-average soil moisture for hydrological and agricultural applications. However, reliance on rare materials such as helium-3 increases the cost of cosmic-ray neutron probes (CRNPs) and limits the adoption of this unique technology beyond the realm of academic research. In this study, we evaluated a novel lower cost CRNP based on moderated ultra-thin lithium-6 foil (Li foil system) technology against a commercially-available CRNP based on BF3 (boron trifluoride, BF-3 system). The study was conducted in a cropped field located in the Konza Prairie Biological Station near Manhattan, Kansas, USA (325 m a.s.l.) from 10 April 2020 to 18 June 2020. During this period the mean atmospheric pressure was 977 kPa, the mean air relative humidity was 70%, and the average volumetric soil water content was 0.277 m3 m−3. Raw fast neutron counts were corrected for atmospheric pressure, atmospheric water vapor, and incoming neutron flux. Calibration of the CRNPs was conducted using four intensive field surveys (n > 120), in combination with continuous observations from an existing array of in situ soil moisture sensors. The time series of uncorrected neutron counts of the Li foil system was highly correlated (r2 = 0.91) to that of the BF-3 system. The Li foil system had an average of 2,250 corrected neutron counts per hour with an uncertainty of 2.25%, values that are specific to the instrument size, detector configuration, and atmospheric conditions. The estimated volumetric water content from the Li foil system had a mean absolute difference of 0.022 m3 m−3 compared to the value from the array of in situ sensors. The new Li foil detector offers a promising lower cost alternative to existing cosmic-ray neutron detection devices used for hectometer-scale soil moisture monitoring.
Radiological and Clinical Findings of Multiple Cerebellar Liponeurocytoma: A Case Report
Authors: Wang Shan , Xu Xiaopei , Wang Chao
Published: July 2021

Background: Cerebellar liponeurocytoma is an extremely rare benign tumor which generally occurs in cerebellum and is almost always solitary. Multifocal cerebellar liponeurocytoma is exceedingly rare, only 8 cases has been reported so far. Herein we present the 9th case of multifocal cerebellar liponeurocytoma in a 70-year-old woman with the complete clinical course and comprehensive imaging findings.

Case Presentation: A 70-year-old woman presented with a history of intermittent headache for 5 years. Computed tomography (CT) and magnetic resonance imaging (MRI) of the brain have been performed and suggested a diagnosis of teratoma based on the imaging findings. After the surgical resection of the lesion, histopathological and immunohistochemical analyses revealed neuronal, glial, and lipomatous components and confirmed the diagnosis of multifocal cerebellar liponeurocytoma after surgical resection. During the 2-year follow-up period, the patient showed no signs of recurrence or metastasis.

Conclusion: We described the radiological characteristics and clinical course of an exceedingly rare case of multifocal cerebellar liponeurocytoma in the cerebellar vermis and temporal lobe. The clear multifocality makes this case unusual.
Bayesian Analysis of the Effects of Olive Oil-Derived Antioxidants on Cryopreserved Buck Sperm Parameters
Authors: Arando Arbulu Ander , Navas González Francisco Javier , Bermúdez-Oria Alejandra , Delgado Bermejo Juan Vicente , Fernández-Prior África , González Ariza Antonio , León Jurado Jose Manuel , Pérez-Marín Carlos Carmelo
Published: July 2021

The present study evaluates the effect of olive oil-derived antioxidants, hydroxytyrosol (HT) and 3,4-dihydroxyphenylglycol (DHPG), on cryopreserved caprine sperm using Bayesian inference of ANOVA. For this proposal, sperm was collected, pooled and diluted in freezing media supplemented with different concentrations of HT, DHPG and the mixture (MIX) of both antioxidants. Sperm motility, viability, acrosome integrity, mitochondrial status, and lipid peroxidation (LPO) were assessed in fresh and frozen-thawed sperm samples. The results provided evidence that HT at low concentrations improves sperm motility and viability, and reduces the LPO. Contrastingly, DHPG and MIX exert a positive effect by reducing LPO values as concentration increases. Additionally, mitochondrial potential was reduced when samples were supplemented with HT at low concentrations and mixture of both antioxidants. Conclusively, the addition of olive oil-derived antioxidants (HT at 10 µg/mL and DHPG at 30 µg/mL) implements a protective effect in cryopreserved buck sperm. Bayesian analysis alternatives offer new possibilities to determine the repercussion of antioxidants on sperm, both quantitatively and qualitatively.
Photo-Aligned Nematic Liquid Crystals Enable the Modulation of Thermoplasmonic Heating
Authors: Palermo Giovanna , Grillo Rossella , Pezzi Luigia , Bürgi Thomas , Tabiryan Nelson , De Sio Luciano , Umeton Cesare
Published: July 2021

We experimentally demonstrate that the plasmonic heat delivered by a single layer of homogeneously distributed gold nanoparticles (AuNPs), immobilized on a glass substrate, can be optically tuned by taking advantage of the properties of an organic layer based on azobenzene and nematic liquid crystal (NLC) molecules. The effect, which exploits the dependence of the NLC refractive index value on the molecular director orientation, is realized using the polarization-dependent, light-induced molecular reorientation of a thin film of photo-aligning material that the NLC is in contact with. The reversibility of the optically induced molecular director reorientation of the NLC enables an active modulation of the plasmonic photo-induced heat.
3D Nanostructured Materials and Devices
Authors: Park Junyong
Published: July 2021

Advances in nanofabrication technology have allowed us to create high-level nanostructured materials that were previously unattainable [...]
WDTISeg: One-Stage Interactive Segmentation for Breast Ultrasound Image Using Weighted Distance Transform and Shape-Aware Compound Loss
Authors: Li Xiaokang , Qiao Mengyun , Guo Yi , Zhou Jin , Zhou Shichong , Chang Cai , Wang Yuanyuan
Published: July 2021

Accurate tumor segmentation is important for aided diagnosis using breast ultrasound. Interactive segmentation methods can obtain highly accurate results by continuously optimizing the segmentation result via user interactions. However, traditional interactive segmentation methods usually require a large number of interactions to make the result meet the requirements due to the performance limitations of the underlying model. With greater ability in extracting image information, convolutional neural network (CNN)-based interactive segmentation methods have been shown to effectively reduce the number of user interactions. In this paper, we proposed a one-stage interactive segmentation framework (interactive segmentation using weighted distance transform, WDTISeg) for breast ultrasound image using weighted distance transform and shape-aware compound loss. First, we used a pre-trained CNN to attain an initial automatic segmentation, based on which the user provided interaction points of mis-segmented areas. Then, we combined Euclidean distance transform and geodesic distance transform to convert interaction points into weighted distance maps to transfer segmentation guidance information to the model. The same CNN accepted the input image, the initial segmentation, and weighted distance maps as a concatenation input and provided a refined result, without another additional segmentation network. In addition, a shape-aware compound loss function using prior knowledge was designed to reduce the number of user interactions. In the testing phase on 200 cases, our method achieved a dice of 82.86 ± 16.22 (%) for automatic segmentation task and a dice of 94.45 ± 3.26 (%) for interactive segmentation task after 8 interactions. The results of comparative experiments proved that our method could obtain higher accuracy with fewer simple interactions than other interactive segmentation methods.
The Effect of Mouthrinse with 0.05% Dexamethasone Solution on the Oral Bacterial Community of Oral Lichen Planus Patients: Prospective Pilot Study
Authors: Ku Jeong-Kui , Park Shin-Young , Hwang Kyung-Gyun , Yun Pil-Young
Published: July 2021

Few studies have already been performed to assess oral bacteria during steroid therapy for oral lichen planus (OLP). Thus, the aim of our study was to analyze the effect of dexamethasone mouthrinse treatment on the oral bacteria of OLP patients. This prospective study was conducted on patients who were diagnosed with OLP and treated with 0.05% dexamethasone mouthrinse twice per day for 4 weeks. Using unstimulated saliva of the patients before and after treatment, the qualitative and quantitative changes in oral bacteria were analyzed using quantitative real-time polymerase chain reaction (qPCR). The qPCR results were analyzed using Wilcoxon signed-rank test to the quantitative changes with dexamethasone mouthrinse. The statistical significance was considered at a level of 0.05. In total, 20 patients were enrolled in this study, wherein all were noted to show improved symptoms of OLP. Fifteen patients (75%) had a qualitative change in the oral microbial species and an improved relative periodontitis risk score (from 26.1 ± 10.7 to 20.9 ± 9.2; p = 0.008). However, quantitative changes in all species were determined to be not statistically different before and after the treatment. Most OLP patients had a changed microbial community composition after 0.05% dexamethasone mouthrinse for 4 weeks. In particular, the composition of the periodontopathic bacteria was improved after the treatment.
A Case Study on User Evaluation of Scientific Publication Summarization by Japanese Students
Authors: Yamamoto Shintaro , Suzuki Ryota , Fukusato Tsukasa , Kataoka Hirokatsu , Morishima Shigeo
Published: July 2021

Summaries of scientific publications enable readers to gain an overview of a large number of studies, but users’ preferences have not yet been explored. In this paper, we conduct two user studies (i.e., short- and long-term studies) where Japanese university students read summaries of English research articles that were either manually written or automatically generated using text summarization and/or machine translation. In the short-term experiment, subjects compared and evaluated the two types of summaries of the same article. We analyze the characteristics in the generated summaries that readers regard as important, such as content richness and simplicity. The experimental results show that subjects are mainly judged based on four criteria, including content richness, simplicity, fluency, and format. In the long-term experiment, subjects read 50 summaries and answered whether they would like to read the original papers after reading the summaries. We discuss the characteristics in the summaries that readers tend to use to determine whether to read the papers, such as topic, methods, and results. The comments from subjects indicate that specific components of scientific publications, including research topics and methods, are important to judge whether to read or not. Our study provides insights to enhance the effectiveness of automatic summarization of scientific publications.
Impact of Chitosan-Genipin Films on Volatile Profile of Wine along Storage
Authors: Rocha M. Angélica M. , Coimbra Manuel A. , Rocha Sílvia M. , Nunes Cláudia
Published: July 2021

Chitosan-genipin films have been proposed for preservation of white wine, maintaining their varietal key odorants and organoleptic characteristics of sulfur dioxide treated wines. Nevertheless, these wines showed aroma notes that slightly distinguish them. It is possible that during the contact of films with wine for at least 2 months, after fermentation and prior to bottling, interactions or chemical reactions are promoted. In this work, wine model solutions with volatile compounds in contact with chitosan-genipin films were performed to evaluate their evolution along time. To complement these analyses, the volatile compounds of white and red wines kept in contact with chitosan-genipin films during 2 and 8 months were also studied. The results obtained allowed us to conclude that the contact of chitosan-genipin films with both white and red wines tend to retain long carbon chain volatile compounds, such as ethyl hexanoate and octan-3-one. It also promoted the formation of Maillard reaction products, such as furfural by dehydration of pentoses and Strecker aldehydes, such as 3-methylbutanal and phenylacetaldehyde, by degradation of amino acids. This study reveals that the use of chitosan-genipin films for wine preservation is also able to promote the formation of compounds that can modulate the wines aroma, maintaining the varietal notes.
Horizontal UHS Amplitudes for Regions with Deep Soil Atop Deep Geological Sediments—An Example of Osijek, Croatia
Authors: Bulajić Borko Đ. , Hadzima-Nyarko Marijana , Pavić Gordana
Published: July 2021

In this paper, we demonstrate how UHS-based seismic microzonation can be applied in low-to-medium seismicity areas with deep local soil and deep geological deposits under the local soil. The case study area surrounds the city of Osijek, Croatia, which is in the south–central region of the Pannonian Basin. New frequency-dependent scaling equations are derived, and the empirical response spectra are compared to the spectra of real strong motions in the surrounding region. Empirical calculations for deep soil atop deep geological strata show a 37% reduction in short-period spectral amplitudes when compared to rock locations. This demonstrates that local soil amplification is mitigated by energy dissipation in deep soils. For vibration periods longer than 0.3 s, spectral amplitudes are being amplified. This amplification goes up to 2.37 times for vibration periods around 0.5 s. UHS spectra for Osijek are computed using regional seismicity estimates, data on local soil and deeper geological surroundings, and newly created regional empirical equations for scaling various spectral amplitudes. UHS amplitudes for Osijek are also compared to the Eurocode 8 spectra for ground type C. The results show that ratios of the maximum UHS amplitudes to PGA values are up to 46% larger than the corresponding 2.5 factor that is recommended by Eurocode 8 for horizontal spectra. The UHS results might be viewed as preliminary for Osijek and regions with similar seismicity and local soil and deep geology conditions. When the number of regional strong-motion records grows many times beyond what it is currently, it will be feasible to properly calibrate the scaling equations, resulting in more reliable and long-term UHS estimations for the area under consideration.
Future Climate Change Impact on Urban Heat Island in Two Mediterranean Cities Based on High-Resolution Regional Climate Simulations
Authors: Keppas Stavros Ch. , Papadogiannaki Sofia , Parliari Daphne , Kontos Serafim , Poupkou Anastasia , Tzoumaka Paraskevi , Kelessis Apostolos , Zanis Prodromos , Casasanta Giampietro , de’Donato Francesca , Argentini Stefania , Melas Dimitrios
Published: July 2021

The Mediterranean is recognized among the most responsive regions to climate change, with annual temperatures projected to increase by 1–5 °C until 2100. Large cities may experience an additional stress discomfort due to the Urban Heat Island (UHI) effect. In the present study, the WRF-ARW numerical weather prediction model was used to investigate the climate change impact on UHI for two Mediterranean cities, Rome and Thessaloniki. For this purpose, three 5-year time-slice simulations were conducted (2006–2010, 2046–2050, 2096–2100) under the Representative Concentration Pathway (RCP) 8.5 emission scenario, with a spatial resolution of 2 km. In order to comprehensively investigate the urban microclimate, we analyze future simulation data across sections crossing urban/non-urban areas, and after grouping them into three classes depending on the location of the grid cells. The urban areas of both cities present increased average minimum temperature (Tmin) in winter/summer compared to other rural areas, with an UHI of ~+1.5–3 °C on average at night/early morning. Considering UHI under future climate change, we found no significant variations (~±0.2 °C). Finally, we found that the numbers of days with Tmin ≥ 20 °C will mostly increase in urban coastal areas until 2100, while the largest increase of minimum Discomfort Index (DImin) is expected in urban low-ground areas.
Entire Analytic Functions of Unbounded Type on Banach Spaces and Their Lineability
Authors: Zagorodnyuk Andriy , Hihliuk Anna
Published: July 2021

In the paper we establish some conditions under which a given sequence of polynomials on a Banach space X supports entire functions of unbounded type, and construct some counter examples. We show that if X is an infinite dimensional Banach space, then the set of entire functions of unbounded type can be represented as a union of infinite dimensional linear subspaces (without the origin). Moreover, we show that for some cases, the set of entire functions of unbounded type generated by a given sequence of polynomials contains an infinite dimensional algebra (without the origin). Some applications for symmetric analytic functions on Banach spaces are obtained.