STUDY OF THE FORMATION OF BIOFILMS OF LISTERIA MONOCYTOGENES IN PLANTS AND MIXED FODDER
Authors:   All-Russian Research Institute of Veterinary Sanitation, Hygiene and Ecology – Branch of Federal State Budget Scientific Institution «Federal Scientific Center – K. I. Skryabin, Ya. R. Kovalenko All-Russian Research Institute of Experimental Veterinary Medicine, Russian Academy of Sciences» Moscow, Russian Federation, Pavlova I.B. , Kononenko A.B. ,   All-Russian Research Institute of Veterinary Sanitation, Hygiene and Ecology – Branch of Federal State Budget Scientific Institution «Federal Scientific Center – K. I. Skryabin, Ya. R. Kovalenko All-Russian Research Institute of Experimental Veterinary Medicine, Russian Academy of Sciences» Moscow, Russian Federation, Bannikova D.A. ,   All-Russian Research Institute of Veterinary Sanitation, Hygiene and Ecology – Branch of Federal State Budget Scientific Institution «Federal Scientific Center – K. I. Skryabin, Ya. R. Kovalenko All-Russian Research Institute of Experimental Veterinary Medicine, Russian Academy of Sciences» Moscow, Russian Federation, Britova S.V. ,   All-Russian Research Institute of Veterinary Sanitation, Hygiene and Ecology – Branch of Federal State Budget Scientific Institution «Federal Scientific Center – K. I. Skryabin, Ya. R. Kovalenko All-Russian Research Institute of Experimental Veterinary Medicine, Russian Academy of Sciences» Moscow, Russian Federation
Published: July 2021

The aim of the work is to study the biofilm formation of L. monocytogenes in plants and mixed fodder. Materials and methods. Oat seeds contaminated with antibiotics and washed from their residues were germinated. Oat seedlings were placed in the soil and grown for 10 days. The near-root zone was contaminated with Listeria suspension and morphology and biofilm formation of Listeria populations in vegetative organs of plants were studied after a certain period of time. Granulated feed was used as mixed fodder, exogenously contaminated with L. monocytogenes suspension. The morphological features of bacterial populations and the formation of biofilms were studied by scanning electron microscopy. Research results. Experimental bacteriological and morphological studies have shown that as a result of the contamination of the Listeria root zone, they penetrated through the capillary system of the roots into the oat leaf blades and were localized in chlorenchyma. In the studies by scanning electron microscopy of preparations obtained from the leaves, on the fragments of the inner part of the oat leaf (devoid of the epidermis), were identified cells with both a typical morphology and at various stages of heteromorphism, and the formation of biofilm was noted. In the study of mixed fodder by method SEM, it was shown that at low temperatures (4 ° C), during the first 24 hours, not only the adhesion of individual cells to the surfaces of the feed particles occurs, but also the formation of microcolonies and colonies often with the formation of biofilms, which indicates the active reproduction of these bacteria. At the same time, it was noted that at temperature of 37 ° C and an exposure of 24 hours, the processes of adhesion and cell colonization of Listeria particles of mixed fodder were slowed down under the same conditions, which seems to be due to the psychrophilicity of Listeria.