Cross-Talk between Leukemic Blasts and T Cells Drives Targetable T Cell Dysfunction in the AML Tumor Microenvironment
Authors: DeWolf Susan , Ceglia Nicholas , Zatzman Matthew , Gipson Brianna , Elhanati Yuval , Katsamakis Zoe , Lees Hannah , Weis Kenyon , Yepes Gustavo , Oliveira Barbara , Longhini Ana Figueiredo , Smith Natalie Grier , Nichols Katherine , Havasov Eliyahu , Zhao Karen , Meskauskaite Brigita , Masilionis Ignas , Lewis Alexander M. , Cuibus Maria Adriana , Pajwani M. Kazim , Minnie Simone A , Chaligne Ronan , Lemarquis Andri Leo L , Goldberg Aaron D. , Hsu Katharine C. , Stein Eytan M. , Greenbaum Benjamin D. , Abdel-Wahab Omar , Shah Sohrab P. , van den Brink Marcel R.M. 
Published: December 2024

Allogeneic hematopoetic cell transplantation harnesses donor T cell alloreactivity against leukemic blasts and is curative in a subset of patients with AML. Aside from transplant, however, T cell based immunotherapies have been unsuccessful in AML and in patients with AML there is evidence for impaired endogenous immune responses. Despite these observations, mechanisms underpinning ineffective anti-leukemic T cell immunity are not fully known.

Here we hypothesized that leukemic blasts drive impaired T cell immunity leading to distinct T cell compositions during different disease states. Using multi-modal approaches to study T cell phenotype and T cell receptor (TCR) repertoire across AML disease states we identified dominant clonally expanded terminal effector memory CD45RA+ (TEMRA) CD8 T cells in the marrow of AML patients with active disease along with abundant immunosuppressive CD4 T regulatory cells (Tregs). CD8 TEMRA clones maintain over time in patients with persistent AML and exhibited numerous interactions with malignant blasts suggestive of ongoing immune modulation by antigen producing leukemic cells. A subset of these CD8 effectors (expressing CX3CR1 and other NK-like markers) exert anti-tumor cytotoxic activity ex vivo but are suppressed by interactions with marrow Tregs. Consistent with this, Treg depletion rescued CD8 effector activity and promoted AML eradication.

To study leukemic blasts and T cell immunity in the AML tumor microenvironment (TME), we performed integrative analysis of protein (CITE-seq and 31-color spectral flow), transcript, and TCRs in individual lymphoid and myeloid cells from longitudinal marrows. We applied this to 179 patient samples from 91 subjects (71 AML, 20 controls), sequencing &gt;670K cells total and &gt;190K T cells. We found that patients harbor a highly abundant CD8 TEMRA population at AML diagnosis that persisted over time in patients who did not achieve remission. These cells express clonally expanded TCRs, a subset of which were marked by CX3CR1, TIGIT (but not PD-1 or TIM3) and attributes of cytotoxicity including granzyme B, perforin, and NK-like markers.

To interrogate the function of expanded effector CD8 T cells, we investigated marrow T cells in an unirradiated syngeneic AML mouse model (C1498 cells into C57B6 mice). As AML accumulated in the marrow, TIGIT+ effector CD8s increased in frequency, as in human AML. A subset of these effectors expressed CX3CR1, which we hypothesized might have tumor killing capability given their cytotoxic profile in patient data. Indeed, in vitro functional analysis revealed increased killing of endogenous tumor by marrow CD8 effectors including CX3CR1+ effectors compared to naïve CD8s harvested 18-20 days post-tumor injection. Importantly in patients with AML we harnessed the TCR CDR3 sequence as a barcode to track phenotypes of CD8 clonotypes over time and found that certain CX3CR1+ TEMRAs transition to a CX3CR1- state in ongoing disease, suggesting loss of cytotoxicity.

Analyses of cell-cell interactions from CITE-seq suggested altered myeloid-T cell interactions in AML compared to control and remission samples, including increased signaling between myeloid cells, Tregs, and memory CD8s in patients with active AML. AML blasts also had increased expression of T cell inhibitory molecules including TIGIT ligands, CD244, and VISTA compared to healthy myeloid cells. Notably, in addition to expanded CD8 TEMRAs, we found increased Tregs in marrow from patients with AML and C1498 engrafted mice. Tregs in both human and mouse AML expressed high levels of TIGIT, CD39, ICOS, and CCR4 but were not clonally expanded. Ex vivo AML marrow Tregs suppressed CD8 effector function. Importantly depletion of Tregs in vivo through transgenic FoxP3 diphtheria toxin receptor mice prolonged host survival, promoted tumor clearance, and led to an increase in marrow CX3CR1+ effector CD8 T cells.

These data demonstrate the active immunologic landscape of the AML bone marrow in both patient samples and mouse models of the disease. We find that although CD8 T cells have the potential for anti-leukemic immunity, their efficacy is impaired by leukemic blasts and suppressive Tregs. These studies suggest Treg-targeting interventions as a therapeutic avenue to overcome the immunosuppressive TME in AML and nominate a host of potentially targetable T cell and blast cell surface proteins that restrain T cell anti-tumor immunity in AML.