bluuebunny/crossref_metadata_2025 huggingface dataset:
Amelioration of oxygen-induced retinopathy in neonatal mice with fetal growth restriction
Authors: Watanabe Ryusuke , Liu Shuang , Sakaue Tomohisa , Ikegawa Yasuhito , Ohta Masaaki , Higaki Takashi , Mogi Masaki , Eguchi Mariko
Published: February 2024

Introduction: With the aim of optimizing the balance of maintaining a safe oxygen saturation and reducing the risk of retinopathy of prematurity in human neonates with fetal growth restriction (FGR), the present study investigated the distinct effects of oxygen supplementation on the retinal neovasculature using a murine premature neonatal oxygen-induced retinopathy (OIR) model with or without fetal growth restriction.

Methods: For comparison with normal birth-weight neonates, maternal low-protein diet-induced FGR neonates were subjected to fluctuating oxygen levels to generate oxygen-induced retinopathy. The retinal neovasculature was histologically evaluated, and comprehensive transcriptome analysis was conducted.

Results: Compared to OIR neonates with normal birth weight, significant amelioration of the neovasculature, as indicated by decreases in the number of branch junctions, vascular distribution, maximal vascular radius and microaneurysm-like tufts, was observed in OIR mice with FGR. The results of retinal RNA-sequencing revealed downregulation of angiogenic factors that trigger pathological retinal neovascularization, such as the mitogen-activated protein kinase pathway and corresponding upstream signaling pathways in OIR mice with FGR.

Conclusion: Our findings demonstrated that FGR neonates have a higher capacity for retinal oxygen stress, and the risk of OIR development is attenuated compared to that in mature neonates with normal birth weight.
Biosocial predictors and blood pressure goal attainment among postmenopausal women with hypertension
Authors: Kandasamy Geetha , Subramani Thangamani , Sam Gigi , Almanasef Mona , Almeleebia Tahani , Shorog Eman , Alshahrani Asma M. , Hmlan Amjad , Al Suhaym Atheer Y. , Prabahar Kousalya , Veeramani Vinoth Prabhu , Amirthalingam Palanisamy
Published: February 2024

Objectives

In postmenopausal states, women may not maintain blood pressure (BP) in the same way as men, even though most women follow their treatment plans and prescriptions more consistently than men. Biological and lifestyle factors influence the progression of hypertension in postmenopausal women (PMW). This study aimed to determine biosocial predictors associated with achieving the target BP in PMW with hypertension.

Methods

A prospective observational study was conducted in the General Medicine Department at Karuna Medical College Hospital, Kerala, India. The definition of BP goal attainment was established based on the guidelines outlined by the VIII Joint National Committee 2014 (JNC VIII). Multivariate logistic regression analysis was used to analyse biosocial predictors, such as educational status, employment status, body mass index (BMI), number of children, age at menarche, age at menopause, and number of co-morbidities, associated with BP goal achievement.

Results

Of the patients, 56.4% achieved their BP goals on monotherapy and 59.7% achieved it on combination therapy. Level of education [odds ratio (OR) = 1.275, 95% confidence interval (CI): 0.234–7.172], employment status (OR = 0.853, 95% CI: 0.400–1.819), age at menopause (OR = 1.106, 95% CI: 0.881–1.149), number of children (OR = 1.152, 95% CI: 0.771–1.720), BMI (OR = 0.998, 95% CI: 0.929–1.071), and number of co-morbidities (OR = 0.068, 95% CI: 0.088–1.093) did not show a significant relationship, and age at menarche (OR = 1.577, 95% CI: 1.031–2.412) showed a significant association with BP goal attainment among hypertensive postmenopausal women.

Conclusion

Half of the hypertensive postmenopausal women did not achieve their BP goals. Interventions are required to expand screening coverage and, under the direction of medical professionals, there should be plans to improve hypertension control and increase awareness of the condition.
The effect of colchicine on cholesterol crystal formation, expansion and morphology: a potential mechanism in atherosclerosis
Authors: Abideen Zain Ul , Pathak Dorothy R. , Sabanci Rand , Manu Megan , Abela George S.
Published: February 2024

Background

Inflammation is pivotal to the progression of atherosclerosis. Cholesterol crystals (CCs) that grow and enlarge within the plaque core can cause plaque rupture and trigger inflammation as they deposit into the atherosclerotic bed. Thus, agents that affect CC formation, expansion, and morphology may reduce cardiovascular (CV) risk independent of lipid-lowering and anti-inflammatory therapy.

Objective

Because colchicine is highly concentrated in leukocytes that can enter the atherosclerotic plaque core, we tested its effect on the formation and growth of CCs in bench experiments to determine whether it may have direct effects on CCs, independent of its known anti-inflammatory actions.

Method

Different dosages of colchicine mixed with cholesterol (0.05–5 mg/ml/g of cholesterol) were used to influence the formation CCs and volume expansion in vitro. These were compared to control samples with cholesterol in ddH2O without colchicine. In an ex vivo study, fresh atherosclerotic human plaques were incubated with and without colchicine in a water bath at 37°C for 48 h to assess the impact of colchicine on CC morphology. Scanning electron microscopy (SEM) was utilized to analyze CC morphology in samples from the various treatment groups.

Results

The addition of colchicine to cholesterol caused a substantial dose-dependent reduction in volume (p < 0.05). Pairwise comparisons of volume reduction, showed a significant reduction in volume at 5 mg/ml/g when compared to control (p < 0.02) but the calculated Cohen's d effect size was large for five of the six pairwise comparisons. By SEM, CCs from both in vitro and ex vivo samples treated with colchicine had evidence of dissolution and changes in their morphology as evidenced by the loss of their sharp edges. In contrast, CCs in untreated specimens retained their typical geometric structure.

Conclusions

Colchicine can reduce CC formation and expansion and alter CC morphology. These previously unappreciated effects of colchicine may contribute to its clinical benefit in patients with CV disease independent of its anti-inflammatory effects.
The effect of surgery started at different time point during the day on the clinical outcomes of mitral valve surgery
Authors: Zheng Shuai , Wang Jiangang , Zhang Haibo , Wang Shengyu , Meng Xu
Published: February 2024

Background

The clinical prognosis of mitral valve surgery at morning, afternoon, and evening is not yet clear. The aim of the study is to investigate the impact of different time periods of surgery in the morning, afternoon and evening on the short-term and long-term results of mitral valve surgery.

Methods

From January 2018 to December 2020, 947 patients with mitral valve surgery in our department were selected. These patients were divided into 3 groups according to the starting time of surgery. Morning group (operation start time 8:00–10:30, n = 231), afternoon group (operation start time 12:00–14:30, n = 543), and evening group (operation start time 17:30–20:00, n = 173). The short-term and long-term results of the three groups were compared.

Results

There were no significant difference in the long-term mortality, long-term risk of stroke and reoperation. And there were no significant difference in in-hospital outcomes, including mortality, stroke, cardiopulmonary bypass time, aortic cross clamp time, mitral valve repair convert to mitral valve replacement, number of aortic cross clamp ≥2 times, unplanned secondary surgery during hospitalization (including thoracotomy hemostasis, thoracotomy exploration, redo mitral valve surgery, and debridement), intra-aortic balloon pump, extracorporeal membrane oxygenation, continuous renal replacement therapy, mechanical ventilation time, and intensive care unit length of stay.

Conclusion

There is no significant difference in the risk of short-term and long-term survival and adverse events after mitral valve surgery at different time periods in the morning, afternoon, and evening. Mitral valve surgery at night is safe.
Elucidating the complex interplay between natural and anthropogenic factors in the deformation of the Muyubao landslide through time-series InSAR analysis
Authors: Yang Rui , Liang Wenli , Yu Chunyong , Kou Pinglang
Published: February 2024

In the Three Gorges Reservoir area, landslide disasters occur frequently, making scientific monitoring and risk prediction crucial for disaster prevention and mitigation. However, most previous studies have been constrained by analysis of singular influencing factors. In this study, we employed multi-temporal InSAR techniques coupled with multivariate geospatial statistical analysis to monitor and analyze the dynamic evolution of the Muyuba landslide in Zigui County, Hubei Province, China from 2016 to 2023. The findings indicate that the Muyuba landslide was predominantly characterized by continuous, gradual subsidence. Key factors inducing deformation included well-developed drainage networks, gentle slopes of 15–30°, and the orientation of rock strata. Deformation rates in residential areas and along roadways exceeded background levels, implicating anthropogenic activities in the heightened landslide risk. A significant correlation was observed between landslide deformation and reservoir water level fluctuations, as opposed to rainfall patterns, highlighting reservoir regulation disturbances as a critical landslide triggering factor.
Geology, fluid inclusions, mineral and (S-O) isotope chemistry of the Badran orogenic Au deposit, Yana-Kolyma belt, eastern Siberia: implications for ore genesis
Authors: Fridovsky Valery , Kryazhev Sergey , Polufuntikova Lena , Kudrin Maxim , Anisimova Galina
Published: February 2024

The Badran orogenic gold deposit is located in the Yana-Kolyma belt, Eastern Siberia; it has proven reserves of ∼9.3 t of gold and an average grade of 7.8 ppm. The total gold production at the Badran deposit since 1984 amounts to ∼34 t. Despite many years of study, the origin of the gold deposits of the Yana-Kolyma metallogenic belt, one of the world’s largest belts, and the Badran deposit is controversial. Synthesis of regional geology and geology of the Badran deposit, fluid inclusion analysis, mineral and (S-O) isotope chemistry defines the genetic model, origin of fluids, and source of metals in the evolution of the ore-forming system, equivalent to other orogenic gold deposits on the margin of the Siberian craton. The deposit is localized in the Upper Triassic clastic rocks and is controlled by the NW-trending thrust. Polyphase mineralization occurs as disseminated arsenian pyrite and arsenopyrite ores with invisible gold, quartz veins with native gold and Fe, Pb, Zn, Cu sulfides and sulfosalts of orogenic type, and locally post-ore Ag, Sb-bearing minerals and Hg epithermal features. The quartz veins with native gold were formed from low-medium saline (1.5–10 wt% NaCl eq.) aqueous-carbonic fluids boiling at temperatures of 290°C to 210 °C and pressures of 300–250 to 125 bar. The δ34S values of pyrite and arsenopyrite vary from −1.1‰ to +2.4‰, with an average of +0.4‰; the δ18О of quartz from +15.1‰ to +17.5‰ at constant δ18ОH2O about +7.5‰ (±1.0‰). High contents of As (up to 2.4 wt%) and Co/Ni ∼ from 0.3 to 9.9 in pyrite of proximal alteration are typical for hydrothermal systems. The results obtained confirm that the ore-forming fluids did not have a single origin, but were formed from a mixture of subcontinental lithospheric mantle and metamorphic sources. The subcrustal lithospheric mantle was fertilized in the time preceding mineralization (Late Jurassic) and was derived directly from the down-going subduction slab and overlying sediment wedge at the closure of the Oymyakon Ocean.
Exquisite skeletons of a new transitional plesiosaur fill gap in the evolutionary history of plesiosauroids
Authors: Sachs Sven , Eggmaier Stefan , Madzia Daniel
Published: February 2024

Plesiosaurs are Mesozoic reptiles fully adapted to an aquatic lifestyle. Throughout their evolutionary history exceeding 140 million years plesiosaurs dispersed globally, achieved substantial diversity, occupied a variety of ecological niches, and experienced multiple faunal turnovers. Of those, the Early/Middle Jurassic transition event (∼175–171 Mya) has recently became of increased interest because it apparently profoundly affected all three major lineages of plesiosaurs. Once dominant Rhomaleosauridae started to vanish, while Pliosauridae and Plesiosauroidea diversified and gave rise to several clades that flourished for tens of millions of years. Here, we report exquisite, three-dimensionally preserved skeletons of a new plesiosaur from the Lower Jurassic of Germany. Franconiasaurus brevispinus gen. et sp. nov. lived during the late Toarcian (∼175 Mya), near the onset of the Early–Middle Jurassic turnover. Franconiasaurus displays an intriguing mixture of features, combining characters almost uniformly distributed among early plesiosaurs with those typically observed in later-diverging members of the clade. Phylogenetic analyses firmly place Franconiasaurus as the sister taxon to Cryptoclidia, bridging an evolutionary gap between early plesiosauroids, such as Plesiosaurus-like forms and microcleidids, and later-diverging representatives of the clade, such as cryptoclidids, leptocleidians, and elasmosaurids.
Corporate environmental infringement, legal regulation, and sustainable development: punitive damages as a perspective
Authors: Li Ziwei , Wang Xingyu , Wu Zhuang
Published: February 2024

In order to maximize personal interests, enterprises have a tendency to choose environmental pollution in exchange for corporate profits, resulting in negative externalities; the Civil Code of the People’s Republic of China imposes legal regulations on the environmental infringement of enterprises in order to achieve the maximization of the use of the allocation of social resources and promote the sustainable development of society. Subsequent judicial interpretations have set a two-fold cap on the amount of punitive damages. However, in cases of extremely serious environmental damage, the two-fold cap on punitive damages for environmental infringement will create the possibility of insufficient compensation. Based on this, this paper analyzes the legal text of China’s punitive damages provisions for environmental infringement and analyzes the interrelationships between enterprises and between enterprises and potential environmental infringement victims in a game analysis. The results of the analysis show that as the amount of punitive damages for environmental infringement increases, the probability of the infringed person filing a lawsuit and that of the enterprise reducing its environmental infringement behavior also increase. In order to promote the cause of China’s sustainable development, this paper puts forward relevant suggestions for the improvement of China’s environmental legal regulation: first, the upper limit of the amount of punitive damages for environmental infringement should be canceled; second, the amount of reasonable punitive damages should be set on the basis of the damages caused by environmental infringement on human beings and the environment; lastly, in the baseline setting of the amount of punitive damages, additional factors need to be taken into account, and the amount of punitive damages should be set for the enterprises with a high degree of maliciousness. Finally, additional factors need to be considered in setting the benchmark for the amount of punitive damages, which should penalize the enterprises causing environmental infringement with a high degree of malice with heavier penalties and, at the same time, penalize the enterprises with a smaller scale of operation and economic strength with lighter penalties.
Zoning strategies for ecological restoration in the karst region of Guangdong province, China: a perspective from the “social-ecological system”
Authors: Liu Yang , Huang Jiajun , Lin Wei
Published: February 2024

Ecological restoration holds great significance in addressing environmental degradation and rock desertification in karst areas. Zoning strategy is a fundamental task in understanding the interrelationship between human-environment to foster sustainable development. We explore “social-ecological” system and conduct a case study on the karst region in Guangdong Province, China. An evaluation framework consists of “development pressure”, “sensitivity status”, and “resilience potential” was established. The results show that: regions with high pressure of development are predominantly located in high-density urban areas. The generally distribution of the comprehensive status index exhibits significant spatial heterogeneity. Regions with low sensitivity are found on the eastern and western sides of the study area. The comprehensive resilience values are largely influenced by per capita energy-saving and environmental protection expenditures. The restoration zones mainly concentrated in the contiguous regions of the northwestern and southern parts, covering more than half of the total area. The conservation zones are more numerous and primarily situated in the northern and eastern parts. By integrating socio-economic and ecological factors, this study proposes ecological restoration strategies for specific zones. It helps for improve development issues arising from complex interactions between human-environment, facilitating the implementation of restoration practices.
Spatiotemporal evolution characteristics and the driving force of habitat quality in the Qinghai–Tibet Plateau in topographic view (2000–2020)
Authors: Yu Haotian , Qian Xuexue , Jing Haichao , Liu Yinghui
Published: February 2024

Introduction

As a key indicator to evaluate biodiversity and eco-function, habitat quality shows the value of ecosystem services. The Qinghai–Tibet Plateau occupies the highest elevation in the global dimension, where the regional environment is greatly affected by multiple factors including the terrain comprehensively.

Methods

In the present study, the InVEST model was employed to assess the habitat quality across varying terrain gradients. The study observed the displacement of the center of gravity of habitat quality under different topographical factors over the past 20 years to learn the trend of changes. Additionally, the Geo-detector model was enhanced to explore the contribution rate of driving factors including biodiversity in different topographic positions and overall.

Results

The results found the following: (1) The habitat quality of the Qinghai–Tibet Plateau was positively correlated with the terrain gradient, and the mean values of habitat quality increased with the gradual increase in terrain gradient. In the period between 2000 and 2020, mean habitat quality values within the study area exhibited a slight fluctuating trend with the spatial distribution characterized by higher values in the southeast and lower values in the northwest. (2) Among different terrain gradients, trends in the mean center’s shift of habitat quality were diverse. The mean center of gradient I went north but gradient V went west. The mean center of the habitat quality in gradients II–IV showed an uncertain trend. (3) The NDVI exerted the most vital influence on the quality of habitats irrespective of different terrain gradients. Annual mean temperature, soil type, and slope occupied two to four places in each terrain.

Discussion

The study indicates that in the last two decades, the habitat quality on the Qinghai–Tibet Plateau has gradually increased across different terrain locations. In terms of policies to protect habitats, terrain should be classified and targeted accordingly.
Analysis of temporal variation characteristics in water resources in typical ecosystems of the Genhe River Basin
Authors: Zhao Kai , Qin Fucang , Yue Yongjie , Li Long , Dong Xiaoyu , Liu Linfu , Li Yan , Wu Yihan , He Rong , Wang Yaqian , Xu Yajie , Wu Yunzhula
Published: February 2024

The Genhe River Basin is an ecological barrier and water conservation area in northern China, but its hydrological process has undergone significant changes due to climate change and human activities, endangering ecosystem functions and water resource security. Systematic research on the influencing mechanisms and laws of hydrological processes in different ecosystems in this region remains lacking. Therefore, this study analyzed the effects of different anthropogenic factors on the hydrological processes of typical ecosystems in the Genhe River Basin. The Soil and Water Assessment Tool distributed hydrological model was used to simulate the surface runoff, evapotranspiration, and soil water content of the three ecosystems of forest, grassland, and farmland in four different periods of 1980, 1990, 2000, and 2010. The spatial and temporal changes in water resources in typical ecosystems under the influence of historical climate change were demonstrated. Results showed that under different land use scenarios, the surface runoff of the farmland ecosystem increased, the evapotranspiration remained unchanged, and the soil water content decreased. The surface runoff of forest and grassland ecosystems did not change significantly, the evapotranspiration increased, and the soil water content decreased. This study reveals the influence of different human factors on the hydrological processes of typical ecosystems in the Genhe River Basin and provides a scientific basis for water resources management and ecological protection in the region.
Spatiotemporal response of ecosystem services to tourism activities in urban forests
Authors: Li Jiadan , Zhang Xian , Gu Qing , Zhang Zhongchu , Wang Kai , Xu Zhihao
Published: February 2024

Tourism in urban forests is rapidly becoming an increasing trend; however, rather few studies have used quantitative measurement to describe the relationship between tourism intensity and ecological functions. This study provides a practical framework that integrates ecosystem service value (ESV) assessment, Internet big data mining and spatial regression analysis to identify the spatial response of ESV and land use/land cover change to tourism activities from 2009 to 2019 in the Siming Mountain Region (SMR), a famous tourist resort located in the eastern coastal China. Results showed that between 2009 and 2019 total ESV increased by 7.1%. Nevertheless, there have been drastic transitions in land use types with function adjustments from traditional agricultural production to diversified tourism-oriented services. Significant spatial autocorrelation was identified for the patterns of ESV changes. GWR further highlighted that the relationship between ESV change and rural tourism indicators varied in space. ESV change in the core zone was negatively correlated with changes in catering service spots and recreational venues, whereas it was positively correlated with local lodgings. Ultimately, targeted recommendations and countermeasures for spatial planning and sustainable tourism development of urban forests under new circumstances were discussed.
Fish conservation in streams of the agrarian Mississippi Alluvial Valley: conceptual model, management actions, and field verification
Authors: Killgore Kenneth J. , Hoover Jan J. , Miranda L. E. , Slack W. T. , Johnson David R. , Douglas Neil H.
Published: February 2024

The effects of agriculture and flood control practices accrued over more than a century have impaired aquatic habitats and their fish communities in the Mississippi Alluvial Valley, the historic floodplain of the Lower Mississippi River prior to leveeing. As a first step to conservation planning and adaptive management, we developed and tested a conceptual model of how changes to this floodplain have affected stream environments and fish assemblages. The model is deliberately simple in structure because it needs to be understood by stakeholders ranging from engineers to farmers who must remain engaged to ensure effective conservation. Testing involved multivariate correlative analyses that included descriptors of land setting, water quality, and fish assemblages representing 376 stream samples taken over two decades and ranging in Strahler stream order from 1 to 8. The conceptual model was adequately corroborated by empirical data, but with unexplained variability that is not uncommon in field surveys where gear biases, temporal biases, and scale biases prevent accurate characterizations. Our conceptual model distinguishes three types of conservation actions relevant to large agricultural floodplains: reforestation of large parcels and riparian zone conservation, in-channel interventions and connectivity preservation, and flow augmentation. Complete restoration of the floodplain may not be an acceptable option to the agriculture community. However, in most cases the application of even the most basic measures can support the return of sensitive aquatic species. We suggest that together these types of conservation actions can bring improved water properties to impacted reaches, higher reach biodiversity, more intolerant species, and more rheophilic fishes.
Systems and computational analysis of gene expression datasets reveals GRB-2 suppression as an acute immunomodulatory response against enteric infections in endemic settings
Authors: Naidu Akshayata , Lulu S. Sajitha
Published: February 2024

Introduction

Enteric infections are a major cause of under-5 (age) mortality in low/middle-income countries. Although vaccines against these infections have already been licensed, unwavering efforts are required to boost suboptimalefficacy and effectiveness in regions that are highly endemic to enteric pathogens. The role of baseline immunological profiles in influencing vaccine-induced immune responses is increasingly becoming clearer for several vaccines. Hence, for the development of advanced and region-specific enteric vaccines, insights into differences in immune responses to perturbations in endemic and non-endemic settings become crucial.

Materials and methods

For this reason, we employed a two-tiered system and computational pipeline (i) to study the variations in differentially expressed genes (DEGs) associated with immune responses to enteric infections in endemic and non-endemic study groups, and (ii) to derive features (genes) of importance that keenly distinguish between these two groups using unsupervised machine learning algorithms on an aggregated gene expression dataset. The derived genes were further curated using topological analysis of the constructed STRING networks. The findings from these two tiers are validated using multilayer perceptron classifier and were further explored using correlation and regression analysis for the retrieval of associated gene regulatory modules.

Results

Our analysis reveals aggressive suppression of GRB-2, an adaptor molecule integral for TCR signaling, as a primary immunomodulatory response against S. typhi infection in endemic settings. Moreover, using retrieved correlation modules and multivariant regression models, we found a positive association between regulators of activated T cells and mediators of Hedgehog signaling in the endemic population, which indicates the initiation of an effector (involving differentiation and homing) rather than an inductive response upon infection. On further exploration, we found STAT3 to be instrumental in designating T-cell functions upon early responses to enteric infections in endemic settings.

Conclusion

Overall, through a systems and computational biology approach, we characterized distinct molecular players involved in immune responses to enteric infections in endemic settings in the process, contributing to the mounting evidence of endemicity being a major determiner of pathogen/vaccine-induced immune responses. The gained insights will have important implications in the design and development of region/endemicity-specific vaccines.
Serum alpha-fetoprotein response as a preoperative prognostic indicator in unresectable hepatocellular carcinoma with salvage hepatectomy following conversion therapy: a multicenter retrospective study
Authors: Lin Kong-Ying , Zhang Jian-Xi , Lin Zhi-Wen , Chen Qing-Jing , Luo Liu-Ping , Chen Jin-Hong , Wang Kui , Tai Sheng , Zhang Zhi-Bo , Wang Shi-Feng , Li Jing-Dong , Wang Kai , Zheng Lu , Zheng Si-Ming , Wu Meng-Meng , Lin Ke-Can , Yang Tian , Zeng Yong-Yi
Published: February 2024

Background

This study evaluates the efficacy of alpha-fetoprotein (AFP) response as a surrogate marker for determining recurrence-free survival (RFS) in patients with unresectable hepatocellular carcinoma (uHCC) who undergo salvage hepatectomy following conversion therapy with tyrosine kinase inhibitor (TKI) and anti-PD-1 antibody-based regimen.

Methods

This multicenter retrospective study included 74 patients with uHCC and positive AFP (>20 ng/mL) at diagnosis, who underwent salvage hepatectomy after treatment with TKIs and anti-PD-1 antibody-based regimens. The association between AFP response—defined as a ≥ 80% decrease in final AFP levels before salvage hepatectomy from diagnosis—and RFS post-hepatectomy was investigated.

Results

AFP responders demonstrated significantly better postoperative RFS compared to non-responders (P<0.001). The median RFS was not reached for AFP responders, with 1-year and 2-year RFS rates of 81.3% and 70.8%, respectively. In contrast, AFP non-responders had a median RFS of 7.43 months, with 1-year and 2-year RFS rates at 37.1% and 37.1%, respectively. Multivariate Cox regression analysis identified AFP response as an independent predictor of RFS. Integrating AFP response with radiologic tumor response facilitated further stratification of patients into distinct risk categories: those with radiologic remission experienced the most favorable RFS, followed by patients with partial response/stable disease and AFP response, and the least favorable RFS among patients with partial response/stable disease but without AFP response. Sensitivity analyses further confirmed the association between AFP response and improved RFS across various cutoff values and in patients with AFP ≥ 200 ng/mL at diagnosis (all P<0.05).

Conclusion

The “20-80” rule based on AFP response could be helpful for clinicians to preoperatively stratify the risk of patients undergoing salvage hepatectomy, enabling identification and management of those unlikely to benefit from this procedure.
Unlocking protein-based biomarker potential for graft-versus-host disease following allogenic hematopoietic stem cell transplants
Authors: Iacobescu Maria , Pop Cristina , Uifălean Alina , Mogoşan Cristina , Cenariu Diana , Zdrenghea Mihnea , Tănase Alina , Bergthorsson Jon Thor , Greiff Victor , Cenariu Mihai , Iuga Cristina Adela , Tomuleasa Ciprian , Tătaru Dan
Published: February 2024

Despite the numerous advantages of allogeneic hematopoietic stem cell transplants (allo-HSCT), there exists a notable association with risks, particularly during the preconditioning period and predominantly post-intervention, exemplified by the occurrence of graft-versus-host disease (GVHD). Risk stratification prior to symptom manifestation, along with precise diagnosis and prognosis, relies heavily on clinical features. A critical imperative is the development of tools capable of early identification and effective management of patients undergoing allo-HSCT. A promising avenue in this pursuit is the utilization of proteomics-based biomarkers obtained from non-invasive biospecimens. This review comprehensively outlines the application of proteomics and proteomics-based biomarkers in GVHD patients. It delves into both single protein markers and protein panels, offering insights into their relevance in acute and chronic GVHD. Furthermore, the review provides a detailed examination of the site-specific involvement of GVHD. In summary, this article explores the potential of proteomics as a tool for timely and accurate intervention in the context of GVHD following allo-HSCT.
Gut microbiota and its metabolic products in acute respiratory distress syndrome
Authors: Zhang Dong-Wei , Lu Jia-Li , Dong Bi-Ying , Fang Meng-Ying , Xiong Xia , Qin Xue-Jun , Fan Xian-Ming
Published: February 2024

The prevalence rate of acute respiratory distress syndrome (ARDS) is estimated at approximately 10% in critically ill patients worldwide, with the mortality rate ranging from 17% to 39%. Currently, ARDS mortality is usually higher in patients with COVID-19, giving another challenge for ARDS treatment. However, the treatment efficacy for ARDS is far from satisfactory. The relationship between the gut microbiota and ARDS has been substantiated by relevant scientific studies. ARDS not only changes the distribution of gut microbiota, but also influences intestinal mucosal barrier through the alteration of gut microbiota. The modulation of gut microbiota can impact the onset and progression of ARDS by triggering dysfunctions in inflammatory response and immune cells, oxidative stress, cell apoptosis, autophagy, pyroptosis, and ferroptosis mechanisms. Meanwhile, ARDS may also influence the distribution of metabolic products of gut microbiota. In this review, we focus on the impact of ARDS on gut microbiota and how the alteration of gut microbiota further influences the immune function, cellular functions and related signaling pathways during ARDS. The roles of gut microbiota-derived metabolites in the development and occurrence of ARDS are also discussed.
Sudocetaxel Zendusortide (TH1902) triggers the cGAS/STING pathway and potentiates anti-PD-L1 immune-mediated tumor cell killing
Authors: Demeule Michel , Currie Jean-Christophe , Charfi Cyndia , Zgheib Alain , Cousineau Isabelle , Lullier Véronique , Béliveau Richard , Marsolais Christian , Annabi Borhane
Published: February 2024

The anticancer efficacy of Sudocetaxel Zendusortide (TH1902), a peptide-drug conjugate internalized through a sortilin-mediated process, was assessed in a triple-negative breast cancer-derived MDA-MB-231 immunocompromised xenograft tumor model where complete tumor regression was observed for more than 40 days after the last treatment. Surprisingly, immunohistochemistry analysis revealed high staining of STING, a master regulator in the cancer-immunity cycle. A weekly administration of TH1902 as a single agent in a murine B16-F10 melanoma syngeneic tumor model demonstrated superior tumor growth inhibition than did docetaxel. A net increase in CD45 leukocyte infiltration within TH1902-treated tumors, especially for tumor-infiltrating lymphocytes and tumor-associated macrophages was observed. Increased staining of perforin, granzyme B, and caspase-3 was suggestive of elevated cytotoxic T and natural killer cell activities. Combined TH1902/anti-PD-L1 treatment led to increases in tumor growth inhibition and median animal survival. TH1902 inhibited cell proliferation and triggered apoptosis and senescence in B16-F10 cells in vitro, while inducing several downstream effectors of the cGAS/STING pathway and the expression of MHC-I and PD-L1. This is the first evidence that TH1902 exerts its antitumor activity, in part, through modulation of the immune tumor microenvironment and that the combination of TH1902 with checkpoint inhibitors (anti-PD-L1) could lead to improved clinical outcomes.
The interplay of co-occurring ecosystem engineers shapes the structure of benthic communities – a mesocosm experiment
Authors: Gusmao Joao Bosco , Rühmkorff Sarah , Kraufvelin Lucinda , Meysick Lukas , Pansch Christian
Published: February 2024

Introduction

Ecosystem engineers play a pivotal role in shaping habitats through their activities and presence. In shallow Baltic waters, seagrasses, patch-forming mussels, and infaunal clams modify soft bottom habitats, impacting benthic community structure. While the individual effects of these ecosystem engineers are well studied, interactions among co-occurring engineers are poorly understood.

Methods

We conducted a mesocosm experiment to assess the independent and combined impacts of seagrass (Zostera marina), epifaunal mussels (Mytilus spp.), and infaunal clams (Macoma balthica) on invertebrate colonization in soft sediments.

Results

Our findings reveal significant engineer-driven alterations in macrofaunal community structure. Combined engineer effects diverged from individual impacts, indicating potential synergies or antagonisms in sediment (re)colonization. Notably, a higher number of engineer species positively affected the diversity of settled macrofauna, with the lowest macrofaunal abundance and biomass but the highest Shannon diversity found in the presence of all three engineers.

Discussion

Results suggest that seagrass, mussels, and clams influence benthos through larval settlement and sediment biogeochemistry, providing insights into the distinct roles of habitat-forming organisms in shaping the benthic communities in coastal ecosystems of the Baltic Sea.
Potentially toxigenic phytoplankton patterns in the northwestern Iberian Peninsula
Authors: Detoni Amália Maria Sacilotto , Navarro Gabriel , Padín Xosé Antonio , Ramirez-Romero Eduardo , Zoffoli Maria Laura , Pazos Yolanda , Caballero Isabel
Published: February 2024

The Galician estuaries are Europe’s foremost supplier of mussels, generating millions of euros annually and offering substantial employment opportunities for its population. One of the most critical threats to shellfish production is the occurrence of harmful algal blooms (HABs), contaminating bivalves with phytotoxins. To successfully tackle this problem, there needs to be a collaborative effort between the scientific community and decision-makers to establish a dynamic and effective monitoring system. This could enable early warnings and preventive actions to avert the loss of millions of tons of shellfish. Remote sensing, despite its limitations, requires commitment and effort by experts to devise effective methods for detecting target optical constituents mixed with other undesired target but that exhibit strong signals. Therefore, the essential necessity arises to identify approaches for mitigating the shortcomings of the undertaken efforts. The objective of this study is to assess the main environmental drivers of potential harmful genera (Pseudo-nitzschia, Dinophysis, Alexandrium, and Gymnodinium) in the Rías Baixas from 2015 to 2022, developing regression-based models and customizing Generalized Additive Models (GAMs) to investigate their spatial-temporal dynamics. Risk-susceptible bloom zones were identified in the river mouth of the Ría Pontevedra and from the center to offshore of the Ría Vigo. Early upwelling events triggered peaks in chlorophyll-a (Chl-a), driving Dinophysis grazing on phytoplankton communities mainly dominated by diatoms, flagellates, and ciliates. Subsequently, the upwelling intensity favoring Pseudo-nitzschia or Alexandrium minutum growth as larger diatoms declined. A. minutum exhibited elevated incidence over the past five years. Gymnodinium catenatum presented a rare occurrence across the three studied estuaries, which limited the assessment of its spatial dynamics in the region. This study emphasizes the need to integrate remote sensing evaluation of high-risk bloom areas (July to September), in-situ cell count collection, and enhanced efforts for forecasting future critical occurrences of HABs.
Diel variation of seawater volatile organic compounds, DMSP-related compounds, and microbial plankton inside and outside a tropical coral reef ecosystem
Authors: Masdeu-Navarro Marta , Mangot Jean-François , Xue Lei , Cabrera-Brufau Miguel , Kieber David J. , Rodríguez-Ros Pablo , Gardner Stephanie G. , Bergauer Kristin , Herndl Gerhard J. , Marrasé Cèlia , Simó Rafel
Published: February 2024

Biogenic volatile organic compounds (VOCs) play key roles in coral reef ecosystems, where, together with dimethylated sulfur compounds, they are indicators of ecosystem health and are used as defense strategies and infochemicals. Assessment and prediction of the exchange rates of VOCs between the oceans and atmosphere, with implications for atmospheric reactivity and climate, are hampered by poor knowledge of the regulating processes and their temporal variability, including diel cycles. Here, we measured the variation over 36h of the concentrations of DMSPCs (dimethylsulfoniopropionate (DMSP)-related compounds, namely DMSP, dimethylsulfoxide, acrylate, dimethylsulfide, and methanethiol as dimethyl disulfide) and VOCs (COS, CS2, isoprene, the iodomethanes CH3I and CH2ClI, and the bromomethanes CHBr3 and CH2Br2), in surface waters inside the shallow, northern coral-reef lagoon of Mo’orea (French Polynesia) and 4 km offshore, in the tropical open ocean. Comparisons with concurrent measurements of sea surface temperature, solar radiation, biogeochemical variables (nutrients, organic matter), and the abundances and taxonomic affiliations of microbial plankton were conducted with the aim to explain interconnections between DMSPCs, VOCs, and their environment across diel cycles. In open ocean waters, deeper surface mixing and low nutrient levels resulted in low phytoplankton biomass and bacterial activity. Consequently, the diel patterns of VOCs were more dependent on photochemical reactions, with daytime increases for several compounds including dissolved dimethylsulfoxide, COS, CS2, CH3I, and CH2ClI. A eukaryotic phytoplankton assemblage dominated by dinoflagellates and haptophytes provided higher cell-associated DMSP concentrations, yet the occurrence of DMSP degradation products (dimethylsulfide, dimethyl disulfide) was limited by photochemical loss. Conversely, in the shallow back reef lagoon the proximity of seafloor sediments, corals and abundant seaweeds resulted in higher nutrient levels, more freshly-produced organic matter, higher bacterial activity, and larger algal populations of Mamiellales, diatoms and Cryptomonadales. Consequently, DMSP and dimethylsulfoxide concentrations were lower but those of most VOCs were higher. A combination of photobiological and photochemical processes yielded sunny-daytime increases and nighttime decreases of dimethylsulfide, dimethyl disulfide, COS, isoprene, iodomethanes and bromomethanes. Our results illustrate the important role of solar radiation in DMSPC and VOC cycling, and are relevant for the design of sampling strategies that seek representative and comparable measurements of these compounds.
Efficacy and safety of small-incision corneal intrastromal lenticule implantation for hyperopia correction: a systematic review and meta-analysis
Authors: Wang Yue , Zheng Jingjing , Guo Zuofeng , Fang Xuejun
Published: February 2024

Purpose

To assess the efficacy and safety of intrastromal lenticule implantation for the treatment of hyperopia.

Methods

A systematic search of PubMed, Web of Science, Embase, Cochrane Library, China National Knowledge Internet, and Wan Fang Database identified studies on small-incision intrastromal lenticule implantation for hyperopia correction until January 2023. The Joanna Briggs Institute (JBI) critical appraisal tool was used to assess the quality of the retrospective research, and the Methodological Index for Non-randomized Studies (MINORS) was used to assess the quality of the prospective research. This study included postoperative visual outcomes, corneal morphology, and biomechanical outcomes.

Results

A total of 456 articles were identified, of which 10 were included in the meta-analysis. Ten single-arm studies involving 190 eyes were included. A meta-analysis demonstrated that corneal intrastromal lenticule implantation treatment significantly improved hyperopia. Uncorrected distance visual acuity (UDVA) significantly improved compared to the preoperative value (p = 0.027), corrected distance visual acuity showed no difference compared to the preoperative value (p = 0.27), and 87% eyes have no loss of one or more lines in the Snellen lines of CDVA (p < 0.00001). There was a significant difference between the spherical equivalent refractive (SE) and preoperative examination (p < 0.00001), 52% of eyes had ±0.5 diopters (D) postoperative SE (p < 0.00001), and 74% eyes had ±1.0 D postoperative SE (p < 0.00001). The central corneal thickness (CCT) increased by 72.68 μm compared to that preoperatively (p < 0.00001), and corneal curvature increased by 4.18D (p < 0.00001). The Q-value decreased by 0.82 (p < 0.00001), and higher-order aberration (HOA) decreased by 0.66 (p < 0.00001).

Conclusion

Small-incision intrastromal lenticule implantation may be an effective solution for correcting hyperopia. The effect of improved vision is significant, but further exploration is needed for changes in corneal biomechanics and long-term safety.

Systematic review registration: https://www.crd.york.ac.uk/PROSPERO/, identifier: CRD42023432343.
Bacterial community changes in strawberry fruits (Fragaria × ananassa variety “Monterey”) from farm field to retail market stands, an indicator of postharvest contamination
Authors: Tenea Gabriela N. , Reyes Pamela
Published: February 2024

Background

Strawberry (Fragaria × ananassa) fruits are vulnerable to bacterial contamination; some species are pathogenic and can affect human health. Comprehending the bacterial composition and diversity at different ripe stages is a key determinant of the fruit health, productivity, and quality.

Methodology

An amplicon metagenomic approach on the 16S rRNA region was used to identify the bacterial diversity in exocarp of fruits collected from a farm field at two ripe stages: breaking (white, phase two) and ripe (red, phase four) and purchased from different retail market stands at ripe (red, phase four, ready-to-eat) stage. Besides, the fruit quality was assessed.

Results

Strawberries carries a high microorganisms diversity, with Pseudomonaceae, Yearsiniaceae, and Hafniaceae being the most abundant families across the samples. Among the groups, Pseudomonaceae and Clostridiaceae were the most abundant families at breaking (phase two) and ripe (phase four), whereas Yearsiniaceae, Hafniaceae, Aeromonadaceae, and Streptococcaceae were the most abundant families in the market group. Although samples from group four-field and market were at the same ripe stage, the bacterial species composition was divergent. Serratia spp. were prevalent (above 60%) in samples collected from the market group, and Pseudomonas (above 70%) species were mostly found in the samples collected from the field settings regardless of the phase. Besides, Escherichia coli and Salmonella enterica were detected in the ready-to-eat samples from both the field and the market, while Enterococcus gallinarum was detected in the samples that originated from the market. Interestingly, Shewanella putrefaciens and Shewanella profunda, two human opportunistic pathogens, were detected in the fruits from the market only. According to alpha and beta diversity analyses, strawberry fruits displayed significant differences (P < 0.05) in bacterial communities within the ripe group, with the samples from the market showing the most bacterial diversity. Although we do not directly correlate the quality attributes with bacterial diversity, the results indicated a clear separation between groups according with their ripe stage and origin.

Conclusion

This study provides a comprehensive framework of the bacterial diversity throughout the transition from unripe to ripe strawberries which may aid in the development of preventative measures to manage the postharvest contamination.