bluuebunny/crossref_metadata_2025 huggingface dataset:
Molecular basis for cellular retinoic acid-binding protein 1 in modulating CaMKII activation
Authors: Nhieu Jennifer , Miller Michelle C. , Lerdall Thomas A. , Mayo Kevin H. , Wei Li-Na
Published: September 2023

Introduction: Cellular retinoic acid (RA)-binding protein 1 (CRABP1) is a highly conserved protein comprised of an anti-parallel, beta-barrel, and a helix-turn-helix segment outside this barrel. Functionally, CRABP1 is thought to bind and sequester cytosolic RA. Recently, CRABP1 has been established as a major mediator of rapid, non-genomic activity of RA in the cytosol, referred to as “non-canonical” activity. Previously, we have reported that CRABP1 interacts with and dampens the activation of calcium-calmodulin (Ca2+-CaM)-dependent kinase 2 (CaMKII), a major effector of Ca2+ signaling. Through biophysical, molecular, and cellular assays, we, herein, elucidate the molecular and structural mechanisms underlying the action of CRABP1 in dampening CaMKII activation.

Results: We identify an interaction surface on CRABP1 for CaMKII binding, located on the beta-sheet surface of the barrel, and an allosteric region within the helix segment outside the barrel, where both are important for interacting with CaMKII. Molecular studies reveal that CRABP1 preferentially associates with the inactive form of CaMKII, thereby dampening CaMKII activation. Alanine mutagenesis of residues implicated in the CaMKII interaction results in either a loss of this preference or a shift of CRABP1 from associating with the inactive CaMKII to associating with the active CaMKII, which corresponds to changes in CRABP1’s effect in modulating CaMKII activation.

Conclusions: This is the first study to elucidate the molecular and structural basis for CRABP1’s function in modulating CaMKII activation. These results further shed insights into CRABP1’s functional involvement in multiple signaling pathways, as well as its extremely high sequence conservation across species and over evolution.
Multi-view and multi-scale behavior recognition algorithm based on attention mechanism
Authors: Zhang Di , Chen Chen , Tan Fa , Qian Beibei , Li Wei , He Xuan , Lei Susan
Published: September 2023

Human behavior recognition plays a crucial role in the field of smart education. It offers a nuanced understanding of teaching and learning dynamics by revealing the behaviors of both teachers and students. In this study, to address the exigencies of teaching behavior analysis in smart education, we first constructed a teaching behavior analysis dataset called EuClass. EuClass contains 13 types of teacher/student behavior categories and provides multi-view, multi-scale video data for the research and practical applications of teacher/student behavior recognition. We also provide a teaching behavior analysis network containing an attention-based network and an intra-class differential representation learning module. The attention mechanism uses a two-level attention module encompassing spatial and channel dimensions. The intra-class differential representation learning module utilized a unified loss function to reduce the distance between features. Experiments conducted on the EuClass dataset and a widely used action/gesture recognition dataset, IsoGD, demonstrate the effectiveness of our method in comparison to current state-of-the-art methods, with the recognition accuracy increased by 1–2% on average.
Breaking the cycle: unraveling the diagnostic, pathophysiological and treatment challenges of refractory migraine
Authors: Robblee Jennifer
Published: September 2023

Background

Refractory migraine is a poorly described complication of migraine in which migraine has chronified and become resistant to standard treatments. The true prevalence is unknown, but medication resistance is common in headache clinic patient populations. Given the lack of response to treatment, this patient population is extremely difficult to treat with limited guidance in the literature.

Objective

To review the diagnostic, pathophysiological, and management challenges in the refractory migraine population.

Discussion

There are no accepted, or even ICHD-3 appendix, diagnostic criteria for refractory migraine though several proposed criteria exist. Current proposed criteria often have low bars for refractoriness while also not meeting the needs of pediatrics, lower socioeconomic status, and developing nations. Pathophysiology is unknown but can be hypothesized as a persistent “on” state as a progression from chronic migraine with increasing central sensitization, but there may be heterogeneity in the underlying pathophysiology. No guidelines exist for treatment of refractory migraine; once all guideline-based treatments are tried, treatment consists of n-of-1 treatment trials paired with non-pharmacologic management.

Conclusion

Refractory migraine is poorly described diagnostically, its pathophysiology can only be guessed at by extension of chronic migraine, and treatment is more the art than science of medicine. Navigating care of this refractory population will require multidisciplinary care models and an emphasis on future research to answer these unknowns.
Multi-parameter MRI based radiomics nomogram for predicting telomerase reverse transcriptase promoter mutation and prognosis in glioblastoma
Authors: Chen Ling , Chen Runrong , Li Tao , Tang Chuyun , Li Yao , Zeng Zisan
Published: September 2023

Objective

To investigate the clinical utility of multi-parameter MRI-based radiomics nomogram for predicting telomerase reverse transcriptase (TERT) promoter mutation status and prognosis in adult glioblastoma (GBM).

Methods

We retrospectively analyzed MRI and pathological data of 152 GBM patients. A total of 2,832 radiomics features were extracted and filtered from preoperative MRI images. A radiomics nomogram was created on the basis of radiomics signature (rad-score) and clinical traits. The performance of the nomogram in TERT mutation identification was assessed using receiver operating characteristic (ROC) curve, calibration curves, and clinical decision curves. Pathologically confirmed TERT mutations and risk score-based TERT mutations were employed to assess patient prognosis, respectively.

Results

The random forest (RF) algorithm outperformed the other two algorithms, yielding the best diagnostic efficacy in differentiating TERT mutations, with area under the curve (AUC) values of 0.892 (95% CI: 0.828–0.956) and 0.824 (95% CI: 0.677–0.971) in the training set and validation sets, respectively. Furthermore, the predictive power of the radiomics nomogram constructed with the rad-score and clinical variables reached 0.916 (95%CI: 0.864, 0.968) in the training set and 0.880 (95%CI: 0.743, 1) in the validation set. Calibration curve and decision curve analysis findings further uphold the clinical application value of the radiomics nomogram. The overall survival of the high-risk subgroup was significantly shorter than that of the low-risk subgroup, which was consistent with the results of the pathologically confirmed TERT mutation group.

Conclusion

The radiomics nomogram could non-invasively provide promising insights for predicting TERT mutations and prognosis in GBM patients with excellent identification and calibration abilities.
Neural representations for multi-context visuomotor adaptation and the impact of common representation on multi-task performance: a multivariate decoding approach
Authors: Song Youngjo , Shin Wooree , Kim Pyeongsoo , Jeong Jaeseung
Published: September 2023

The human brain's remarkable motor adaptability stems from the formation of context representations and the use of a common context representation (e.g., an invariant task structure across task contexts) derived from structural learning. However, direct evaluation of context representations and structural learning in sensorimotor tasks remains limited. This study aimed to rigorously distinguish neural representations of visual, movement, and context levels crucial for multi-context visuomotor adaptation and investigate the association between representation commonality across task contexts and adaptation performance using multivariate decoding analysis with fMRI data. Here, we focused on three distinct task contexts, two of which share a rotation structure (i.e., visuomotor rotation contexts with −90° and +90° rotations, in which the mouse cursor's movement was rotated 90 degrees counterclockwise and clockwise relative to the hand-movement direction, respectively) and the remaining one does not (i.e., mirror-reversal context where the horizontal movement of the computer mouse was inverted). This study found that visual representations (i.e., visual direction) were decoded in the occipital area, while movement representations (i.e., hand-movement direction) were decoded across various visuomotor-related regions. These findings are consistent with prior research and the widely recognized roles of those areas. Task-context representations (i.e., either −90° rotation, +90° rotation, or mirror-reversal) were also distinguishable in various brain regions. Notably, these regions largely overlapped with those encoding visual and movement representations. This overlap suggests a potential intricate dependency of encoding visual and movement directions on the context information. Moreover, we discovered that higher task performance is associated with task-context representation commonality, as evidenced by negative correlations between task performance and task-context-decoding accuracy in various brain regions, potentially supporting structural learning. Importantly, despite limited similarities between tasks (e.g., rotation and mirror-reversal contexts), such association was still observed, suggesting an efficient mechanism in the brain that extracts commonalities from different task contexts (such as visuomotor rotations or mirror-reversal) at multiple structural levels, from high-level abstractions to lower-level details. In summary, while illuminating the intricate interplay between visuomotor processing and context information, our study highlights the efficiency of learning mechanisms, thereby paving the way for future exploration of the brain's versatile motor ability.
A retrospective comparative study on the diagnostic efficacy and the complications: between CassiII rotational core biopsy and core needle biopsy
Authors: Xie Shuduo , Ju Siwei , Zhang Xun , Qi Chao , Zhang Jiahang , Mao Misha , Chen Cong , Chen Yongxia , Ji Feiyang , Zhou Jichun , Wang Linbo
Published: September 2023

Accurate pathologic diagnosis and molecular classification of breast mass biopsy tissue is important for determining individualized therapy for (neo)adjuvant systemic therapies for invasive breast cancer. The CassiII rotational core biopsy system is a novel biopsy technique with a guide needle and a “stick-freeze” technology. The comprehensive assessments including the concordance rates of diagnosis and biomarker status between CassiII and core needle biopsy were evaluated in this study. Estrogen receptor (ER), progesterone receptor (PgR), human epidermal growth factor receptor 2 (HER2), and Ki67 were analyzed through immunohistochemistry. In total, 655 patients with breast cancer who underwent surgery after biopsy at Sir Run Run Shaw Hospital between January 2019 to December 2021 were evaluated. The concordance rates (CRs) of malignant surgical specimens with CassiII needle biopsy was significantly high compared with core needle biopsy. Moreover, CassiII needle biopsy had about 20% improvement in sensitivity and about 5% improvement in positive predictive value compared to Core needle biopsy. The characteristics including age and tumor size were identified the risk factors for pathological inconsistencies with core needle biopsies. However, CassiII needle biopsy was associated with tumor diameter only. The CRs of ER, PgR, HER2, and Ki67 using Cassi needle were 98.08% (kappa, 0.941; p<.001), 90.77% (kappa, 0.812; p<.001), 69.62% (kappa, 0.482; p<.001), and 86.92% (kappa, 0.552; p<.001), respectively. Post-biopsy complications with CassiII needle biopsy were also collected. The complications of CassiII needle biopsy including chest stuffiness, pain and subcutaneous ecchymosis are not rare. The underlying mechanism of subcutaneous congestion or hematoma after CassiII needle biopsy might be the larger needle diameter and the effect of temperature on coagulation function. In summary, CassiII needle biopsy is age-independent and has a better accuracy than CNB for distinguishing carcinoma in situ and invasive carcinoma.
CD98 heavy chain as a prognostic biomarker and target for cancer treatment
Authors: Xia Pu , Dubrovska Anna
Published: September 2023

The SLC3A2 gene encodes for a cell-surface transmembrane protein CD98hc (4F2). CD98hc serves as a chaperone for LAT1 (SLC7A5), LAT2 (SLC7A8), y+LAT1 (SLC7A7), y+LAT2 (SLC7A6), xCT (SLC7A11) and Asc1 (SLC7A10) providing their recruitment to the plasma membrane. Together with the light subunits, it constitutes heterodimeric transmembrane amino acid transporters. CD98hc interacts with other surface molecules, such as extracellular matrix metalloproteinase inducer CD147 (EMMPRIN) and adhesion receptors integrins, and regulates glucose uptake. In this way, CD98hc connects the signaling pathways sustaining cell proliferation and migration, biosynthesis and antioxidant defense, energy production, and stem cell properties. This multifaceted role makes CD98hc one of the critical regulators of tumor growth, therapy resistance, and metastases. Indeed, the high expression levels of CD98hc were confirmed in various tumor tissues, including head and neck squamous cell carcinoma, glioblastoma, colon adenocarcinoma, pancreatic ductal adenocarcinoma, and others. A high expression of CD98hc has been linked to clinical prognosis and response to chemo- and radiotherapy in several types of cancer. In this mini-review, we discuss the physiological functions of CD98hc, its role in regulating tumor stemness, metastases, and therapy resistance, and the clinical significance of CD98hc as a tumor marker and therapeutic target.
Irrigation scheduling for potatoes (Solanum tuberosum L.) under drip irrigation in an arid region using AquaCrop model
Authors: Yin Juan , Yang Yingpan , Eeswaran Rasu , Yang Zhen , Ma Zhenghu , Sun Fubin
Published: September 2023

Potato is one of the key food crops and China is the largest potato producer in the world. However water scarcity is the major constraint to increase the productivity of potato in the arid regions such as Ningxia in northwest China where this crop is extensively cultivated. The overall objective of this study was to optimize the irrigation for potato cultivated under the drip irrigation. To do this, the AquaCrop model was calibrated and validated using the data obtained from two years of field experiment. Then, the calibrated crop model was used to simulate growth and tuber yield of potato in response to 30 different irrigation schemes under two different irrigation scenarios. The crop model evaluation parameters namely, the root mean square error (RMSE), the index of agreement (d), the normalized root mean square error (NRMSE) and the coefficient of determination (R2) showed that the AquaCrop model could simulate the growth and yield of potato under the drip irrigation with different irrigation treatments with reasonable accuracy. Furthermore, yield of potato has increased with increasing amount of total irrigation under drip irrigation; however, yield begins to decline when the amount of total irrigation exceeds 2500 m3 ha-1. The study also found that the optimum irrigation schedule for potato was 20 mm of irrigation quota at 7 days of irrigation cycle (i.e., 1800 m3 ha-1 or 180 mm of total irrigation). The above irrigation scheduling has achieved 46.77 t ha-1 of tuber yield with 15.74 kg m-3 of water use efficiency. These findings may be evaluated in potato cultivation across different climate and soil conditions for wide applicability at different arid regions of the world.
True, justified, belief? Partisanship weakens the positive effect of news media literacy on fake news detection
Authors: Sude Daniel Jeffrey , Sharon Gil , Dvir-Gvirsman Shira
Published: September 2023

To investigate how people assess whether politically consistent news is real or fake, two studies (N = 1,008; N = 1,397) with adult American participants conducted in 2020 and 2022 utilized a within-subjects experimental design to investigate perceptions of news accuracy. When a mock Facebook post with either fake (Study 1) or real (Study 2) news content was attributed to an alternative (vs. a mainstream) news outlet, it was, on average, perceived to be less accurate. Those with beliefs reflecting News Media Literacy demonstrated greater sensitivity to the outlet’s status. This relationship was itself contingent on the strength of the participant’s partisan identity. Strong partisans high in News Media Literacy defended the accuracy of politically consistent content, even while recognizing that an outlet was unfamiliar. These results highlight the fundamental importance of looking at the interaction between user-traits and features of social media news posts when examining learning from political news on social media.
Chinese EFL learners’ empowerment: does teacher care and confirmation matter?
Authors: Li Min , Chu Zhesen
Published: September 2023

Introduction

Inspired by the rhetorical/relational goal theory, the current inquiry evaluated the role of two teacher relational behaviors, namely care and confirmation, in predicting Chinese EFL learners’ empowerment.

Methods

To do this, we administered three closed-ended scales to 391 Chinese students who studying English as a foreign language in various educational institutions in China. Students’ attitudes toward the interplay between teacher care, teacher confirmation, and learner empowerment were statistically analyzed using IBM SPSS Amos software.

Results

The results uncovered that teacher confirmation and teacher care serve an essential role in improving Chinese EFL learners’ empowerment. This indicates that EFL learners’ empowerment strictly depends on the relational behaviors that teachers employ in instructional-learning contexts.

Discussion

The study outcomes may have some theoretical and practical implications for L2 researchers, language teachers, and teacher trainers.
Graphic health warnings and plain packaging in the Philippines: results of online and household surveys
Authors: Amul Gianna Gayle H. , Mallari Eunice U. , Arda John Rafael Y. , Santiago Alen Josef A.
Published: September 2023

In line with Article 11 of the WHO Framework Convention on Tobacco Control, the Philippines has implemented graphic health warnings on cigarette packs. To date, there has been no published literature evaluating the perceived effectiveness of GHWs in the Philippines. This study aims to contribute to the evidence on the perceived effects of graphic health warnings (GHWs) in cigarette packaging and the potential impact of plain packaging in the Philippines. The study involved an online convenience survey and a nationwide household survey. Mock-up sets of cigarette packs based on the Philippines’ law on GHWs, and Thailand’s and Singapore’s plain packaging were shown to respondents to rate their attractiveness, quality, taste, cost, social appeal, appeal to youth, noticeability, appeal to non-smokers, attempt to quit, ease of quitting, discouraging smoking, and perceived harm to health on a five-point Lickert scale. The online and household surveys recruited 2,701 respondents in total. Online and household survey respondents considered plain packaging with larger graphic health warnings and visible quitlines from Thailand and Singapore to be more effective in discouraging them from smoking. Both sets of survey respondents also found mock-ups from Thailand and Singapore more motivating for them to attempt quitting than cigarette pack mock-ups from the Philippines. The study concludes that current graphic health warnings in the Philippines are ineffective in instilling health consciousness among Filipinos. Policymakers should consider larger graphic health warnings and plain packaging of cigarettes to motivate smokers to quit and discourage Filipinos from smoking.
Association between intoxication with psychoactive substances and adverse effects occurrence in consumers
Authors: Amanollahi Alireza , Shadnia Shahin , Mehrabi Yadollah , Etemad Koorosh
Published: September 2023

Background

The prevalence of psychoactive substance use is increasing worldwide and identifying adverse effects of these types of drugs is necessary in intoxicated patients.

Objective

We aimed to investigate the association of psychoactive substance intoxication with their adverse effects on the functioning of the bodily organs.

Methods

This was a single-center study between March 2019 and April 2022 on intoxicated patients with psychoactive substances. Inclusion criteria were intoxication with alcohol, opioids, and stimulants, and having available results of laboratory biomarkers. Demographic and clinical data of patients at the time of admission as well as during hospitalization were reviewed, retrospectively. Data were analyzed using a generalized linear mixed model in R software and the Adjusted Odds Ratio (AOR) was estimated.

Results

A total of 800 hospitalized patients in the ICU (n = 400) and general ward (n = 400) were divided into two groups of intoxicated with alcohol (n = 200) and opioids or stimulants (n = 200). Liver (AOR = 0.15, p = 0.033; AOR = 0.13, p = 0.007) and kidney (AOR = 0.46, p = 0.004; AOR = 0.24, p = 0.021) dysfunction occurred less in the ICU and general ward, respectively, in opioids or stimulants intoxication compared to alcohol. Cardiovascular dysfunctions occurred more in opioids or stimulants intoxication compared to alcohol in both ICU (AOR = 10.32, p < 0.0001) and general ward (AOR = 4.74, p < 0.0001).

Conclusion

Kidney dysfunctions had a greater effect on mortality compared to other dysfunctions. During the follow-up, the incidence of dysfunctions increased in those intoxicated with opioids or stimulants. Men experienced more liver and kidney dysfunctions as well as mortality, but psychoactive substance experience was a protective factor in cardiovascular dysfunctions and mortality.
Reflections on the Prospective Outcomes of Injury Study (POIS; 2006-2023): how population-based research can address Māori outcomes and governance
Authors: Wyeth Emma H. , Derrett Sarah
Published: September 2023

Injury is a leading cause of disability. Twenty years ago, we knew financial costs of injury were high but little was known about the short, medium and long-term outcomes after injury. In 2006, a Pilot Study and engagement with Māori across the country was undertaken to discuss the planned main study to understand how best to design a study that was meaningful and beneficial to Māori and policy-makers. Between 2007–2009, 2,856 injured New Zealanders (including 20% Māori) with an Accident Compensation Corporation (ACC) entitlement claim were recruited to the Prospective Outcomes of Injury Study (POIS). Participants shared detailed information (at 3, 12 and 24 months, and 12-years post-injury) about a broad range of topics including: the injury, socio-demographics, health, health services access, employment and wellbeing. Administrative data about injury-related hospitalisations, the sentinel injury and subsequent injuries were also collected, as well as in-depth qualitative interviews. This paper focuses on the why, how and impacts of POIS, especially in relation to Māori design and approaches, capability and capacity building, and leadership. Focusing on these aspects for Māori within POIS over time has ensured delivery of findings capable of informing and improving outcomes and policy. In particular, POIS has had considerable impact, influencing ACC's research strategy and outcomes' focus, and has provided disability, health, and wellbeing outcomes knowledge previously unavailable, especially for Māori.
Designing for change through “reflecting and doing”: the CGIAR Community of Practice on Gender-Transformative Research Methodologies
Authors: Lopez Diana E. , Bailey Arwen , Farnworth Cathy Rozel , Rietveld Anne , Gartaula Hom
Published: September 2023

Gender-transformative change requires a commitment from everyone involved in agricultural research for development (AR4D) including organizations at international and national level, individual researchers and practitioners, farmers, development agencies, policy-makers and consumers, to transform the existing values, practices and priorities that (re)produce and perpetuate gender biases and inequities in agrifood systems. However, the adoption of a gender transformative agenda can be challenging, especially for AR4D organizations whose primary focus is not necessarily the attainment of gender equality. This paper looks at a collective, bottom-up, transformative effort within the AR4D organization of CGIAR. It advances the emerging CGIAR Community of Practice on Gender Transformative Research Methodologies (GTRM-CoP) as a case study to explore the potential of CoPs as social learning systems that create the conditions for transformation-oriented learning. Driven by an ethos of reflecting and doing anchored in critical and feminist principles and social learning praxis, the GTRM-CoP aims to be a safe space to spur reflexivity, creativity and collaboration to support existing work on gender transformation in CGIAR while re-imagining how gender in AR4D is conceptualized, negotiated and advanced. The paper focuses on the process leading to the development of the CoP, that is, designing for change, which is crucial for sustained transformation.
Artificial intelligence in postharvest agriculture: mapping a research agenda
Authors: Fadiji Tobi , Bokaba Tebogo , Fawole Olaniyi Amos , Twinomurinzi Hossana
Published: September 2023

Introduction

The implementation of artificial intelligence (AI) in postharvest agriculture has significantly improved in recent decades, thanks to extensive scientific research. The study aimed to identify research gaps and hotspots for future research based on keyword co-occurrence and clustering analyses, as well as to discuss the results and highlight the research trends.

Methods

This study analyses research trends in AI application in postharvest agriculture using novel scientometric tools such as the Bibliometrix R package, biblioshiny, and VosViewer. The research analysed 586 published papers on AI application in postharvest agriculture research between 1994 and September 2022, retrieved from the Scopus database.

Results and discussion

The results showed that publications on AI applications in postharvest agriculture research have been increasing for almost 30 years, with significant growth in the subject area in the last decade. China, the USA, and India were found to be the top three most productive countries, accounting for 52.4%, 22%, and 18.6% of the total selected publications, respectively. The analysis also revealed that topics such as the Internet of Things, cold chain logistics, big data, decision-making, and real-time monitoring have low development degrees in the knowledge domain. This study demonstrated increased research on AI applications in postharvest agriculture, aiming to reduce postharvest losses, enhance food nutrition and quality, and mitigate food insecurity. It also provides valuable scientific references on AI applications in postharvest agriculture research for researchers and scholars. By identifying research gaps and hotspots, this study can guide future research in AI applications in postharvest agriculture to further improve the industry.
Evolving a mitigation of the stress response pathway to change the basic chemistry of life
Authors: Tolle Isabella , Oehm Stefan , Hoesl Michael Georg , Treiber-Kleinke Christin , Peil Lauri , Bozukova Mihaela , Albers Suki , Adamu Bukari Abdul-Rahman , Semmler Torsten , Rappsilber Juri , Ignatova Zoya , Gerstein Aleeza C. , Budisa Nediljko
Published: September 2023

Despite billions of years of evolution, there have been only minor changes in the number and types of proteinogenic amino acids and the standard genetic code with codon assignments across the three domains of life. The rigidity of the genetic code sets it apart from other aspects of organismal evolution, giving rise to key questions about its origins and the constraints it places on innovation in translation. Through adaptive laboratory evolution (ALE) in Escherichia coli, we aimed to replace tryptophan (Trp) in the genetic code with an analogue L-β-(thieno[3,2-b]pyrrolyl)alanine ([3,2]Tpa). This required Escherichia coli to recruit thienopyrrole instead of indole and allowed reassignment of UGG codons. Crossing the stress response system emerged as a major obstacle for ancestral growth in the presence of [3,2]Tp and Trp limitation. During ALE, a pivotal innovation was the deactivation of the master regulon RpoS, which allowed growth solely in the presence of [3,2]Tp in minimal medium. Notably, knocking out the rpoS gene in the ancestral strain also facilitated growth on [3,2]Tp. Our findings suggest that regulatory constraints, not just a rigid translation mechanism, guard Life’s canonical amino acid repertoire. This knowledge will not only facilitate the design of more effective synthetic amino acid incorporation systems but may also shed light on a general biological mechanism trapping organismal configurations in a status quo.
Effect of source and journey on physiological variables in calves transported by road and ferry between Ireland and the Netherlands
Authors: van Dijk Luca L. , Siegmann Susanne , Field Niamh L. , Sugrue Katie , van Reenen Cornelis G. , Bokkers Eddie A. M. , Sayers Gearoid , Conneely Muireann
Published: September 2023

This study aimed to establish baseline variables for calves transported by road and ferry from Ireland to the Netherlands and to investigate the effect of journey [two comparable journeys in April (J1) and May (J2) 2022] and source [source farm or mart (SF/MA)] on these variables. A total of 66 calves from the SF/MA were transported from Ireland to commercial veal farms in the Netherlands. Blood samples were collected at the SF/MA, assembly center (Ireland), lairage (France), and on arrival on the veal farm (Netherlands). They were analyzed for indicator variables related to energy balance, hydration/electrolytes, physical/muscular stress, immunity, and inflammation [glucose, beta-hydroxybutyrate (BHB), non-esterified fatty acids (NEFA), potassium, sodium, magnesium, chloride, urea, haematocrit, total protein, creatine kinase, L-lactate, cortisol, white blood cell, neutrophil, lymphocyte and monocyte counts, serum amyloid-A, and haptoglobin]. Health variables eye and nose discharge, skin tent (a measure of dehydration), and navel inflammation were scored by a trained observer, and calves were weighed at every blood-sampling time point. All blood variables and body weight changed significantly (P < 0.05) during transport, most notably between the assembly center and lairage. Reference ranges were available for 18 variables; 11 of these variables exceeded the reference ranges at the lairage, whilst 10 variables exceeded the reference ranges on arrival at the veal farm. However, health variables did not change during transport. A journey-to-journey comparison indicated much variation; 18 out of 25 variables differed significantly on at least one time point. In total, J1 calves experienced a more severe change in BHB, potassium, strong-ion-difference, L-lactate, and eye and nose discharge than J2 calves. The source of calves also affected their physiology; 12 out of 25 variables studied differed significantly, all of which were confined to the first time point. Specifically, MA calves had elevated levels of NEFA, urea, haematocrit, L-lactate, cortisol, white blood cell, neutrophil, and monocyte counts and lower levels of corrected chloride and lymphocyte count. Overall, calves in this study showed a generalized physiological disturbance beyond reference limits during long-distance transport, but no animal died during transport or for 3 weeks post-arrival.
Case report: Fatal neonatal sepsis associated with Escherichia fergusonii infection in a common bottlenose dolphin (Tursiops truncatus)
Authors: Baek Su-Min , Lee Seoung-Woo , Kim Tae-Un , Kim Ji Hyung , Lee Young-Jin , Yim Jae-Hyuk , Kim Woo Jun , Choi Seong-Kyoon , Han Jee Eun , Jeong Kyu-Shik , Park Jin-Kyu
Published: September 2023

A 25-day-old male common bottlenose dolphin (Tursiops truncatus) died suddenly while swimming at a dolphinarium. The gross examination revealed ulceration on the dorsal and pectoral fins and rostrum. Severe congestion, hemorrhage, and edema were observed in the gastrointestinal tract, liver, mesenteric lymph nodes, lungs, and kidneys. Fibrinosuppurative arthritis of the atlantooccipital joint and extension of fibrin into the spinal canal caused compression of the spinal cord. Histopathological examination revealed tracheitis, fibrinosuppurative bronchopneumonia and enteritis. In the central nervous system, meningeal vessel congestion in the brain, and intraparenchymal hemorrhages with neurodegeneration were observed in the spinal cord. Based on the histopathological findings, representative samples, including lung, liver, mesenteric lymph node, blood obtained from the jugular vein, and fluid sample of the ascites, were inoculated on tryptic soy agar and blood agar for routine bacterial isolation. Each isolated bacterial colony was streaked aseptically onto tryptic soy agar and blood agar for pure culture. After then, polymerase chain reaction (PCR) was performed for further identification of pathogenic microorganisms. PCR identified Escherichia fergusonii, Shewanella haliotis, Enterococcus faecalis, and Staphylococcus schleiferi. E. fergusonii was considered the primary etiologic agent in this case since it was the only species identified in all representative samples. The cause of death in this animal was E. fergusonii sepsis. To the best of our knowledge, this is the first case of neonatal sepsis associated with E. fergusonii infection in a dolphin, and suggests E. fergusonii as an opportunistic pathogen associated with sepsis in dolphins.
Association Between Pandemic Fatigue and Disease Knowledge, Attitudes, Concerns, and Vaccination Intention at Two Key Moments of the COVID-19 Pandemic
Authors: Agurto-Ramírez Adriana , Pino-Rosón Candela , Ayala Alba , Falcón María , Rodríguez-Blázquez Carmen , Forjaz Maria João , Romay-Barja María
Published: September 2023

Objective:This study aimed to describe the change in knowledge, attitudes, concerns, perceptions, preventive practices, and vaccination intention at two key time points of the COVID-19 pandemic and to assess whether these changes varied by level of pandemic fatigue.

Methods:Data included in this study came from the third and the ninth round of the COSMO-Spain cross-sectional study. A general linear model was used to investigate the interaction terms between rounds and levels of pandemic fatigue.

Results:Changes between rounds were observed in knowledge, attitudes, concerns, perceptions, behaviours, and vaccination intention. Significant interactions between rounds indicated that those with low levels of pandemic fatigue had a greater increase in knowledge, lower decrease in concerns, greater decrease in agreement with the decisions made, and lower increase in vaccination intention compared with those with high pandemic fatigue.

Conclusion:As a pandemic evolves, it becomes necessary to consider the level of pandemic fatigue of the population and how this affects knowledge, concerns, and agreement with the measures adopted, as they influence the population’s adherence to public health recommendations aimed at controlling infections and protecting the most vulnerable.
Uncertainty-Estimation-Based Prescribed Performance Pressure Control for Train Electropneumatic Brake Systems
Authors: Zhang Rui , Xu Zejun , Yang Yingze , Zhu Peidong
Published: September 2023

Fast and precise pressure control for an electropneumatic brake system is essential for ensuring the safe operation of trains. However, the nonlinearity and uncertainties of the system make controller design challenging. This paper proposes a prescribed performance control method integrating an extended state observer to address this issue. A thermodynamical model of the brake cylinder is first built based on the pneumatic characteristics of the braking system, considering multiple modes, coupling effects, and input saturation. Then, an extended state observer is designed to estimate model uncertainty due to temperature variation and disturbances and to achieve online compensation of the model. A feedback control law with a specified prescribed performance function is developed based on the updated thermodynamic model to guarantee the transient and steady-state performance of the pressure control. A parameter adaptive method is also utilized to handle input saturation. The observer’s bounded convergence and stability analysis of the closed-loop control system is given using the Lyapunov theory. Compared experimental results are provided to verify the effectiveness of the proposed method.
Evaluating the Antioxidant Properties of the Leaves and Stems of Alpinia oxyphylla In Vitro and Its Growth-Promoting, Muscle Composition Change, and Antioxidative Stress Function on Juvenile Litopenaeus vannamei
Authors: Li Jun-Tao , Zhao Yu-Hua , Lv Yuan , Su Xin , Mei Wen-Li , Lu Yao-Peng , Zheng Pei-Hua , Zhang Ze-Long , Zhang Xiu-Xia , Chen Hui-Qin , Dai Hao-Fu , Xian Jian-An
Published: September 2023

Alpinia oxyphylla is a homology of medicine and food. This study aims to investigate the dominant chemical composition and explore the antioxidant properties of the ethanol extract of the leaves and stems of A. oxyphylla (AOE) on juvenile shrimp, Litopenaeus vannamei. An in vitro test showed that AOE and its dominant chemical composition procyanidin B-2 (1) and epicatechin (2) presented DPPH and ABTS radical scavenging activities. A shrimp feeding supplement experiment revealed that shrimp growth parameters and muscle composition were improved significantly when fed with a 200 mg/Kg AOE additive. Meanwhile, the activities of antioxidant enzymes (CAT, GSH-Px, SOD, and T-AOC) in serum and the liver and the expression of related genes (LvMn-SOD, LvCAT, LvproPo, and LvGSH-Px) were enhanced with various degrees in different AOE additive groups while the content of MDA was significantly decreased. Moreover, the antioxidative effect of AOE additive groups on shrimp was also observed in an acute ammonia nitrogen stress test.
Genetic Modulation of HPV Infection and Cervical Lesions: Role of Oxidative Stress-Related Genes
Authors: Inácio Ângela , Aguiar Laura , Rodrigues Beatriz , Pires Patrícia , Ferreira Joana , Matos Andreia , Mendonça Inês , Rosa Raquel , Bicho Manuel , Medeiros Rui , Bicho Maria Clara
Published: September 2023

Human papillomavirus (HPV) infection is a necessary but not sufficient factor for the development of invasive cervical cancer (ICC) and high-grade intraepithelial lesion (HSIL). Oxidative stress is known to play a crucial role in HPV infection and carcinogenesis. In this study, we comprehensively investigate the modulation of HPV infection, HSIL and ICC, and ICC through an exploration of oxidative stress-related genes: CβS, MTHFR, NOS3, ACE1, CYBA, HAP, ACP1, GSTT1, GSTM1, and CYP1A1. Notably, the ACE1 gene emerges as a prominent factor with the presence of the I allele offering protection against HPV infection. The association of NOS3 with HPV infection is perceived with the 4a allele showing a protective effect. The presence of the GSTT1 null mutant correlates with increased susceptibility to HPV infection, HSIL and ICC, and ICC. This study also uncovers intriguing epistatic interactions among some of the genes that further accentuate their roles in disease modulation. Indeed, the epistatic interactions between the BB genotype (ACP1) and DD genotype (ECA1) were shown to increase the risk of HPV infection, and the interaction between BB (ACP1) and 0.0 (GSTT1) was associated with HPV infection and cervical lesions. These findings underscore the pivotal role of four oxidative stress-related genes in HPV-associated cervical lesions and cancer development, enriching our clinical understanding of the genetic influences on disease manifestation. The awareness of these genetic variations holds potential clinical implications.
Contemplating Light: Experiencing Victor Moscoso’s Psychedelic Lithographs in the Museum
Authors: Barton Aleisha
Published: September 2023

Beginning in 1966, Victor Moscoso designed many of his psychedelic posters for the stroboscopic light shows of the San Francisco dance halls. Moscoso innovated a new mode of print that depended on its environment—kinetic lithography, a product of creative experimentation. He developed multiple iterations of this medium; however, installing it outside of its original context of the psychedelic dance hall continues to pose a unique challenge for preparators and curators alike. Today, museum display of his works relies upon experimental settings to activate his site-specific design. This article considers how immersive displays and antistatic artworks demand a new kind of relationship between visitor and artwork by decentering the museum’s longstanding emphasis on the optical, a regime that has long served to frame posters and ephemera in contexts of display rather than as active objects. By analyzing two recent exhibitions displaying Moscoso’s kinetic lithographs (The Summer of Love Experience, 2017, and Moscoso Cosmos, 2021), this article considers the mechanics of the print itself, curatorial decisions, and visitor engagement to assess the site-specific demands of a genre-bending medium.
Novel Genetically Engineered Probiotics for Targeted Elimination of Pseudomonas aeruginosa in Intestinal Colonization
Authors: Kim Hyun , Jang Ju Hye , Jung In Young , Kim Ha Rang , Cho Ju Hyun
Published: September 2023

The intestinal carriage rates of Pseudomonas aeruginosa are notably elevated in immunosuppressed individuals and hospitalized patients, increasing the risk of infection and antibiotic-associated diarrhea. A potential solution to this issue lies in autonomous antibacterial therapy, remaining inactive until a pathogen is detected, and releasing antibacterial compounds on demand to eliminate the pathogen. This study focuses on the development of genetically engineered probiotics capable of detecting and eradicating P. aeruginosa by producing and secreting PA2-GNU7, a P. aeruginosa-selective antimicrobial peptide (AMP), triggered by the presence of P. aeruginosa quorum-sensing molecule N-(3-oxododecanoyl)-L-homoserine lactone (3OC12HSL). To achieve this goal, plasmid-based systems were constructed to produce AMPs in response to 3OC12HSL and secrete them into the extracellular medium using either the microcin V secretion system or YebF as a carrier protein. Following the transfer of these plasmid-based systems to Escherichia coli Nissle 1917 (EcN), we successfully demonstrated the ability of the engineered EcN to express and secrete PA2-GNU7, leading to the inhibition of P. aeruginosa growth in vitro. In addition, in a mouse model of intestinal P. aeruginosa colonization, the administration of engineered EcN resulted in reduced levels of P. aeruginosa in both the feces and the colon. These findings suggest that engineered EcN holds promise as a potential option for combating intestinal P. aeruginosa colonization, thus mitigating the risk of future endogenous infections in vulnerable patients.
Response Profiles of BV2 Microglia to IFN-γ and LPS Co-Stimulation and Priming
Authors: Pan Meng Liy , Ahmad Puzi Nur Nabilah , Ooi Yin Yin , Ramasamy Rajesh , Vidyadaran Sharmili
Published: September 2023

(1) Background: The latest research illustrates that microglia phenotype is not the binary ‘resting’ and ‘activated’ profiles. Instead, there is wide diversity in microglia states. Similarly, when testing different stimulation protocols for BV2 microglia, we discovered differences in the response of the cells in terms of the production of intracellular ROS (iROS), nitric oxide (NO), CD40 expression, and migratory capacity. (2) Methods: BV2 microglia were treated with single interferon gamma (IFN-γ) stimulation, LPS/IFN-γ co-stimulation, and priming with IFN-γ followed by stimulation with LPS for 24 h. The responses of BV2 microglia were then assessed using the H2DCFDA test for iROS, the Griess assay for NO, immunophenotyping for CD40/CD11b/MHC II, and migration using a transwell apparatus. (3) Results: Single stimulation with IFN-γ induced NO but not ROS in BV2 microglia. Co-stimulation with LPS200IFN-γ2.5 induced a higher iROS production (a 9.2-fold increase) and CD40 expression (28031 ± 8810.2 MFI), compared to priming with primedIFN-γ50LPS100 (a 4.0-fold increase in ROS and 16764 ± 1210.8 MFI of CD40). Co-stimulation also induced cell migration. On the other hand, priming BV2 microglia (primedIFN-γ50LPS100) resulted in a higher NO production (64 ± 1.4 µM) compared to LPS200IFN-γ2.5 co-stimulation (44 ± 1.7 µM). Unexpectedly, priming inhibited BV2 migration. (4) Conclusions: Taken together, the findings from this project reveal the ability of co-stimulation and priming in stimulating microglia into an inflammatory phenotype, and the heterogeneity of microglia responses towards different stimulating approaches.
Effects of Fly Ash Composition to Mitigate Conversion of Calcium Aluminate Cement Composites
Authors: Win Thwe Thwe , Panwisawas Chinnapat , Jongvivatsakul Pitcha , Pansuk Withit , Prasittisopin Lapyote
Published: September 2023

Calcium aluminate cement (CAC) is one of the alternative cements that is widely used for special applications. However, during the hydration process degradation of CAC microstructure, the so-called hydrate conversion process, hexagonal calcium aluminate hydrate (CAH10) transforms into a cubic (C3AH6) phase, resulting in increased porosity and reduced strengths. It is known that alternative means for stabilizing the CAC conversion are conducted by introducing fly ash (FA) in CAC, where its microstructure is attributed to aluminosilicates. However, no study has yet been conducted on different FA compositions influencing CAC performance. This study aims to evaluate the effects of different compositions of FA on CACs’ fresh and hardened characteristics. Results revealed that the microstructure was denser when CAC was mixed with FA. Regarding reactivity, CAC with calcium-rich FA systems is 13% faster than the silica-rich one. The higher the density and the lower the porosity of calcium-rich FA mixtures were found compared with silica-rich FA in both micro- and macro-structures. As seen in the microscopic structure, this is due to the calcium-rich phase formation.