bluuebunny/crossref_metadata_2025 huggingface dataset:
Phosphorus Availability and Uptake following a Maize-Pigeon Pea Rotation under Conservation Agriculture
Authors: Phiri Mirriam , Mulder Jan , Chishala Benson H. , Chabala Lydia M. , Martinsen Vegard
Published: January 2024

Soils on many smallholder farms in Southern Africa are severely depleted in plant nutrients, in particular phosphorus (P), following years of maize monocropping with little or no fertilizer input. Past studies suggest that pigeon pea (Cajanus cajan (L.) Millsp.) may increase plant-available P. Pigeon pea is not a common crop in much of Southern Africa, and the effect of locally grown pigeon pea varieties on plant-available P is unknown. We assessed the changes in plant-available P after growing pigeon pea varieties MPPV-2, MPPV-3, and Babati White in Zambia, viz. Lixisols of Choma and Mkushi, Acrisols of Chipata and Kasama, and Arenosols of Kaoma. The selected soils were not fertilized. Baseline soils (0–20 cm), sampled after long-term maize monocropping and soils from the same fields after growing pigeon pea were collected from field trials in Kaoma, Chipata, Choma, and Mkushi and analyzed for plant-available P. Further, a greenhouse study was conducted with soils from Kasama, Choma, Kaoma, and Chipata, under which soil P was determined before and after growing pigeon pea, soybean (Dina), and maize (SC 419) without fertilizer addition. Pigeon pea under field studies had no significant (p > 0.05) effect on plant-available P in Choma, Kaoma, and Chipata. In Mkushi, pigeon pea cropping resulted in a 47.5% significant decline (p ≤ 0.05) in plant-available P, amounting to a loss of 11.2 kg ha−1. The greenhouse study showed a significant decline (p ≤ 0.001) in plant-available P after seven weeks of maize growth, while there was no significant (p > 0.05) effect on plant-available P after soybean and pigeon pea cropping. The latter was primarily due to the significantly higher P uptake associated with larger biomass production of maize after seven weeks in the greenhouse. During the initial seven weeks, pigeon pea biomass had significantly higher P concentrations than maize. Thus, P deficiency symptoms were exhibited in maize, while pigeon pea appeared healthy. However, mobilized P, calculated as the sum of plant P and soil P after cropping minus soil P before planting, was significantly lower (p ≤ 0.01) in pigeon pea compared to soybean and maize. Synthesizing field and greenhouse experiments suggests that there is a low net decline of plant-available P from soils after pigeon pea cropping. Therefore, rotation with these pigeon pea varieties could be beneficial to resource-poor farmers due to low P removal and its ability to grow in P-deficient soil.
Computational Integral Imaging Reconstruction Based on Generative Adversarial Network Super-Resolution
Authors: Wu Wei , Wang Shigang , Chen Wanzhong , Qi Zexin , Zhao Yan , Zhong Cheng , Chen Yuxin
Published: January 2024

To improve acquisition efficiency and achieve super high-resolution reconstruction, a computational integral imaging reconstruction (CIIR) method based on the generative adversarial network (GAN) network is proposed. Firstly, a sparse camera array is used to generate an elemental image array of the 3D object. Then, the elemental image array is mapped to a low-resolution sparse view image. Finally, a lite GAN super-resolution network is presented to up-sample the low-resolution 3D images to high-resolution 3D images with realistic image quality. By removing batch normalization (BN) layers, reducing basic blocks, and adding intra-block operations, better image details and faster generation of super high-resolution images can be achieved. Experimental results demonstrate that the proposed method can effectively enhance the image quality, with the structural similarity (SSIM) reaching over 0.90, and can also reduce the training time by about 20%.
Gasification Performance of Barley Straw Waste Blended with Lignite for Syngas Production under Steam or Carbon Dioxide Atmosphere
Authors: Vamvuka Despina , Zacheila Konstantina
Published: January 2024

The gasification performance of lignite/barley straw mixtures for syngas production was investigated. The experiments were carried out under a steam or carbon dioxide atmosphere, in fixed-bed and thermogravimetric–mass spectrometry systems. The thermal behavior, reactivity, conversion, product gas composition, liquid and gaseous by-products and interactions between fuels were determined and correlated with the structural characteristics and inherent minerals in ashes, which were analyzed via mineralogical, chemical and fusibility tests. Devolatilization of the materials up to 600 °C resulted in the carbon enrichment of chars and a 30–90-fold increase in the specific surface area. Gaseous and liquid by-products with higher heating values of 5–7 MJ/m3 and 20–28 MJ/kg could offer valuable energy. Upon steam gasification up to 1000 °C, product gas was enriched in hydrogen and carbon monoxide. The syngas yield and heating value of the gas mixture were higher for barley straw fuel (0.77 m3/kg, 11.4 MJ/m3), which, when blended with the lignite, produced upgraded products. Upon carbon dioxide gasification up to 1000 °C, barley straw char exhibited a 3-times higher rate than the lignite, as well as higher conversion (94.5% vs. 62.9%) and a higher syngas yield (0.84 m3/kg vs. 0.55 m3/kg). Lignite/barley straw blends showed synergistic effects and presented higher gasification reactivity and conversion in comparison to lignite. The overall performance of lignite was improved with the steam reagent, while that of barley straw was improved with the carbon dioxide reagent.
Recent Developments in Enantioselective Scandium-Catalyzed Transformations
Authors: Pellissier Hélène
Published: January 2024

This review collects the recent developments in the field of enantioselective scandium-catalyzed transformations published since the beginning of 2016, illustrating the power of chiral scandium catalysts to promote all types of reactions.
Understanding and Mitigating the Purchase Intention of Medicines Containing Saiga Antelope Horn among Chinese Residents: An Analysis of Influencing Factors
Authors: Zhang Mengyuan , Zhang Zhongyi , Chen Junfeng , Cirella Giuseppe T. , Xie Yi
Published: January 2024

The unsustainable demand for wildlife and its derivatives poses a threat to global biodiversity, requiring attention and intervention. This study investigates the intent to purchase medicines containing saiga antelope horn among respondents in China. Drawing on an expanded theoretical framework rooted in a planned behavior model, and analyzing 576 valid data points collected through online research, this paper employs a structural equation model to consider influencing factors across six dimensions: attitude, subjective norm, perceived behavioral control, consumption experience, protective cognitive level, and personal characteristics. The findings reveal that 31.25% of respondents harbor a positive purchase intention toward medicines containing saiga antelope horn. Additionally, attitude, subjective norm, perceived behavioral control, and consumption experience exhibit positive associations with the purchase intention, while the protective cognitive level demonstrates a negative impact. A significant gender gap was identified, with women displaying a greater inclination to purchase compared to men. To support the global conservation efforts of the saiga antelope, this paper advocates for strategic interventions. Recommendations include reinforcing public science education, fostering awareness, advancing the research and development of alternative medicines, strengthening internal market controls, and employing targeted marketing strategies to shift consumer preferences. These measures collectively contribute to a holistic approach aimed at reducing the demand for wildlife products and safeguarding the saiga antelope population.
The Cent Fonts Aquifer: An Overlooked Subterranean Biodiversity Hotspot in a Stygobiont-Rich Region
Authors: Prié Vincent , Alonso Cédric , Bou Claude , Galassi Diana Maria Paola , Marmonier Pierre , Dole-Olivier Marie-José
Published: January 2024

The South of France is a biodiversity hotspot within Europe. Here, we present a comprehensive review of surveys conducted in the Cent Fonts aquifer, an overlooked subterranean biodiversity hotspot embedded in a region rich in stygobiotic species and threatened by climate change and water abstraction projects. Key studies, spanning from 1950 to 2006, show a progression in survey methods and results, although troglobiotic species remain poorly documented. With 43 stygobiotic species recorded, the Cent Fonts is the richest stygobiont hotspot in France. Most species are regional endemics, a quarter of which are considered vulnerable by the IUCN. The Cent Fonts also hosts several relict species and is the type locality of four species. Such a high biological value clearly deserves to be preserved. Our analysis warns of a possible decline in biodiversity, as eight of the species recorded in the 20th century were absent from the 2006 survey, suggesting potential threats of unknown origin. The capture of the Cent Font springs for water abstraction is discussed as a potential threat to this ecosystem and its unique biodiversity. Three new species of stygobiotic molluscs are described, one of which was collected in the Cent Fonts.
Enhancing Exchange-Traded Fund Price Predictions: Insights from Information-Theoretic Networks and Node Embeddings
Authors: Choi Insu , Kim Woo Chang
Published: January 2024

This study presents a novel approach to predicting price fluctuations for U.S. sector index ETFs. By leveraging information-theoretic measures like mutual information and transfer entropy, we constructed threshold networks highlighting nonlinear dependencies between log returns and trading volume rate changes. We derived centrality measures and node embeddings from these networks, offering unique insights into the ETFs’ dynamics. By integrating these features into gradient-boosting algorithm-based models, we significantly enhanced the predictive accuracy. Our approach offers improved forecast performance for U.S. sector index futures and adds a layer of explainability to the existing literature.
Urban Greening in the Process of Climate Change Adaptation of Large Cities
Authors: Pancewicz Alina , Kurianowicz Anna
Published: January 2024

Cities—being places where both growth at large and human activity concentrate to the maximum extent, as well as being places of creation, innovation, and development—have been facing the challenge of adaptation to changing climate conditions. Successive greening of urban spaces is becoming an indicator of civilisational progress and one of the most important aspects of sustainable urban development and quality of life of city dwellers. It also represents a part of the sustainable management of natural resources and energy in the urban environment. This article addresses the subject of urban space greening, perceived as one of the ways of mitigating the effects of climate change. The study focuses on a comparative analysis of various planning and implementing activities related to green areas, as well as on available quantitative data on the greening and climate change adaptation of 44 Polish cities with urban adaptation plans (UAPs) in place. The research, covering the years 2017–2023, identifies the variety of urban greening methods, initiatives, and tools used in the process of urban planning and urban design. The results of the study showed that measures planned by local authorities lacked detailed tools as well as a long-term and systemic approach to greenery, energy, and space management. On the other hand, what was observed in the implementation was an apparent prevalence of urban acupuncture spots and a failure to make the most of all the benefits of the adaptive, social, and ecosystem-forming role of urban greening. The authors imply that their research can be used to formulate conclusions and guidelines for urban development policies which highlight the role and raise the importance of the greening of Polish cities under all urban investments, particularly in the process of adaptation of urban areas to climate change.
Trends for Stirling Engines in Households: A Systematic Literature Review
Authors: Kubule Anna , Kramens Jānis , Bimbere Madara , Pedišius Nerijus , Blumberga Dagnija
Published: January 2024

Reliable heat and power supply are among the basic household needs nowadays. It is especially topical in rural or distant locations that may be cut off from the energy grid due to extreme weather or other events. Nonetheless, the sustainability of our power production systems has to be considered to sustain our planet’s long-term abilities to provide energy resources and a viable environment. Renewable energy resources must be prioritized in rural and remote areas, simultaneously strengthening distributed production ability and self-sufficiency. In this context, the use of the Stirling engine for heat to power generation in households, on the scale of small communities, and by using only renewable resources is becoming increasingly topical. Therefore, this research aims to identify the current state-of-the-art for Stirling engine applications using biomass as a renewable energy source, in the context of sustainability and energy security. The paper summarizes the current research tendencies at the household level in the use of biomass-based Stirling engines for renewable heat and power generation in decentralized energy systems. The methodology applied is a structured literature review and content analysis. The research results corroborate the progress towards the use of renewable resources and towards increased energy efficiency because the keyword and overlay analysis showed more frequent publishing in these areas. The content analysis on two more specific sub-directions of interest, i.e., biomass-fuelled Stirling engine use in households and Stirling engine implications on energy security, showed that though research activity in these directions has increased lately, more profound research is needed, especially on aspects of energy security and independence. The content analysis revealed a lack of in-depth analysis on the effects of Stirling engine use on energy security or energy independence, which is suggested as a topical subject for future research.
Enhancing Loadability of Transmission Lines Using Static Synchronous Series Compensator Devices: A Case Study of the Syrian Network
Authors: Asper Hussam , Shabaan Faisal , Kherbek Tarek , Mohammed Nabil
Published: January 2024

In response to global energy demand, the enhancement of transport capacity in electrical transmission lines is deemed essential. The conventional method of constructing new lines is considered costly, time-consuming, and subject to constraints imposed by economic and environmental factors. Among various emerging solutions aimed at enhancing the passive capacity of transmission lines, the technology of flexible AC transmission systems (FACTS) has been demonstrated to be highly effective. This paper proposes the utilization of the static synchronous series compensator (SSSC), a closed-loop control system and a type of FACTS, to alleviate the overloading of transmission lines in the Syrian electrical network. To achieve this objective, the network is first modeled to identify the overloaded transmission lines. Subsequently, the particle swarm optimization (PSO) algorithm is employed for the optimal sizing and allocation of SSSCs within the network. The findings showcase the significant reduction in loads on critically overloaded transmission lines subsequent to the successful implementation of SSSC devices. This serves to validate the improvements made to the existing infrastructure of the Syrian electrical network’s transmission lines, without necessitating the construction of new transmission lines.
Forest Fuel Bed Variation in Tropical Coastal Freshwater Forested Wetlands Disturbed by Fire
Authors: Barrios-Calderón Romeo de Jesús , Infante Mata Dulce , Flores Garnica José Germán , Torres Jony R.
Published: January 2024

Tropical coastal freshwater forested wetlands in coastal regions are rapidly disappearing as a result of various disturbance agents, mainly wildfires caused by high accumulations of forest fuels. The objective of this study was to characterize the structure and composition of fuel beds in tropical coastal freshwater forested wetlands with three levels of disturbance at El Castaño, La Encrucijada Biosphere Reserve. Seventeen sampling units were used to describe the structure of the forest’s fuel beds (canopy, sub-canopy, and understory). Fallen woody material and litter (surface and fermented) were characterized using the planar intersection technique. Diversity comprised eight species of trees, two shrubs, five lianas, and two herbaceous species. The vertical strata were dominated by trees between 2 and 22 m in height. The horizontal structure had a higher percentage of trees with normal diameter between 2.5 and 7.5 cm (61.4%) of the total. Sites with low disturbance had the highest arboreal density (2686 ind. ha−1). Diversity of species showed that the Fisher, Margalef, Shannon, and Simpson α indices were higher in the low disturbance sites. The Berger–Parker index exhibited greater dominance in the sites with high disturbance. Pachira aquatica Aubl. Showed the highest importance value index and was the largest contributor to fuel beds. Sites with the highest disturbance had the highest dead fuel load (222.18 ± 33.62 Mg ha−1), with woody fuels of classes 1, 10, and 1000 h (rotten) being the most representative. This study contributes to defining areas prone to fire in these ecosystems and designing prevention strategies.
Afforestation, Natural Secondary Forest or Dehesas? Looking for the Best Post-Abandonment Forest Management for Soil Organic Carbon Accumulation in Mediterranean Mountains
Authors: Cortijos-López Melani , Sánchez-Navarrete Pedro , Lasanta Teodoro , Cammeraat Erik L. H. , Nadal-Romero Estela
Published: January 2024

Forest expansion in Mediterranean mountain areas is a widespread phenomenon resulting from the abandonment of agricultural and pastoral activities during the last century. Therefore, knowledge of the long-term storage capacity of soil organic carbon (SOC) in Mediterranean forests is of great interest in the context of global change. However, the effects of these land uses and covers (natural secondary forest, afforestation with conifers and silvo-pastoral ecosystems (dehesas)) on SOC dynamics are still uncertain. The main objectives of this study were to evaluate physico-chemical soil properties, SOC and nitrogen stocks, and SOC fractions in Mediterranean forests and to assess the effects of tree species, the soil environment (acidic and alkaline), and land management. We selected five land uses and land covers: managed and non-managed afforestation and dehesa (except for alkaline dehesa) and a stage of succession when tree species begin to become established after abandonment. This study concludes that although total SOC stocks are higher in afforested systems with conifers, SOC is stored in less stable carbon pools than in broadleaf forests. In addition, this study confirms that there are marked differences in the results between acidic and alkaline environments. Finally, the management system is also a significant factor, particularly for afforested sites.
Tracheids vs. Tree Rings as Proxies for Dendroclimatic Reconstruction at High Altitude: The Case of Pinus sibirica Du Tour
Authors: Zharkov Mikhail S. , Yang Bao , Babushkina Elena A. , Zhirnova Dina F. , Vaganov Eugene A. , Shishov Vladimir V.
Published: January 2024

Siberian pine (Pinus sibirica Du Tour) is a widespread and long-lived species in the northern hemisphere, which makes it a good potential proxy for climatic data. However, the tree-ring growth of this species weakly correlates with climatic conditions, which prevents its use in dendroclimatic reconstruction. It was proposed to use the measurements of tracheid characteristics as model predictors to reconstruct the smoothed temperature of the key periods in tree growth. In this study, algorithms for preprocessing tracheids and temperature data, as well as for model cross-validation, were developed to produce reliable high-resolution (weekly-based) temperature reconstructions. Due to the developed algorithms, the key time periods of Siberian pine growth were identified during the growing season—early June (most active cell development) and mid-July (setting new buds for the next growing season). For these time periods, reliable long-term temperature reconstructions (R2 > 0.6, p < 10−8) were obtained over 1653–2018. The temperature reconstructions significantly correlated (p < 10−8) with independent reanalysis data for the 19th century. The developed approach, based on preprocessing tracheid and temperature data, shows new potential for Siberian pine in high-resolution climate reconstructions and can be applied to other tree species that weakly respond to climate forcing.
Hypertensive Disorders of Pregnancy and Pre-Pregnancy Hypertension with Subsequent Incident Venous Thromboembolic Events
Authors: Malek Angela M. , Wilson Dulaney A. , Turan Tanya N. , Mateus Julio , Lackland Daniel T. , Hunt Kelly J.
Published: January 2024

Hypertensive disorders of pregnancy (HDP) and pre-pregnancy hypertension contribute to maternal morbidity and mortality. We examined the association of HDP and pre-pregnancy hypertension with subsequent venous thromboembolic (VTE) events. The retrospective cohort study included 444,859 women with ≥1 live, singleton birth in South Carolina (2004–2016). Hospital and emergency department visit and death certificate data defined incident VTE, HDP, and pre-pregnancy hypertension. Birth certificate data also defined the exposures. Adjusted Cox proportional hazards methods modeled VTE events risk. Of the cohort, 2.6% of women had pre-pregnancy hypertension, 5.8% had HDP, 2.8% had both pre-pregnancy hypertension and HDP (both conditions), and 88.8% had neither condition. The risk of incident VTE events within one year of delivery was higher in women with HDP (hazard ratio [HR] = 1.62, 95% confidence interval [CI]: 1.15–2.29) and both conditions (HR = 2.32, 95% CI: 1.60–3.35) compared to those with neither condition as was the risk within five years for women with HDP (HR = 1.35, 95% CI: 1.13–1.60) and for women with both conditions (HR = 1.82, 95% CI: 1.50–2.20). One- and five-year risks did not differ in women with pre-pregnancy hypertension compared to women with neither condition. Compared to non-Hispanic White (NHW) women with neither condition, the incident VTE event risk was elevated within five years of delivery for NHW (HR = 1.29, 95% CI: 1.02–1.63; HR = 1.59, 95% CI: 1.16–2.17) and non-Hispanic Black (NHB; HR = 1.51, 95% CI: 1.16–2.96; HR = 2.08, 95% CI: 1.62–2.66) women with HDP and with both conditions, respectively, and for NHB women with pre-pregnancy hypertension (HR = 1.50, 95% CI: 1.09–2.07). VTE event risk was highest in women with HDP, and the event rates were higher in NHB women than in NHW women in the same exposure group.
Differential Proteoglycan Expression in Atherosclerosis Alters Platelet Adhesion and Activation
Authors: Drysdale Amelia , Blanco-Lopez Maria , White Stephen J. , Unsworth Amanda J. , Jones Sarah
Published: January 2024

Proteoglycans are differentially expressed in different atherosclerotic plaque phenotypes, with biglycan and decorin characteristic of ruptured plaques and versican and hyaluronan more prominent in eroded plaques. Following plaque disruption, the exposure of extracellular matrix (ECM) proteins triggers platelet adhesion and thrombus formation. In this study, the impact of differential plaque composition on platelet function and thrombus formation was investigated. Platelet adhesion, activation and thrombus formation under different shear stress conditions were assessed in response to individual proteoglycans and composites representing different plaque phenotypes. The results demonstrated that all the proteoglycans tested mediated platelet adhesion but not platelet activation, and the extent of adhesion observed was significantly lower than that observed with type I and type III collagens. Thrombus formation upon the rupture and erosion ECM composites was significantly reduced (p < 0.05) compared to relevant collagen alone, indicating that proteoglycans negatively regulate platelet collagen responses. This was supported by results demonstrating that the addition of soluble biglycan or decorin to whole blood markedly reduced thrombus formation on type I collagen (p < 0.05). Interestingly, thrombus formation upon the erosion composite displayed aspirin sensitivity, whereas the rupture composite was intensive to aspirin, having implications for current antiplatelet therapy regimes. In conclusion, differential platelet responses and antiplatelet efficacy are observed on ECM composites phenotypic of plaque rupture and erosion. Proteoglycans inhibit thrombus formation and may offer a novel plaque-specific approach to limit arterial thrombosis.
Serum-Induced Proliferation of Human Cardiac Stem Cells Is Modulated via TGFβRI/II and SMAD2/3
Authors: Schmidt Kazuko E. , Höving Anna L. , Kiani Zahrani Sina , Trevlopoulou Katerina , Kaltschmidt Barbara , Knabbe Cornelius , Kaltschmidt Christian
Published: January 2024

The ageing phenotype is strongly driven by the exhaustion of adult stem cells (ASCs) and the accumulation of senescent cells. Cardiovascular diseases (CVDs) and heart failure (HF) are strongly linked to the ageing phenotype and are the leading cause of death. As the human heart is considered as an organ with low regenerative capacity, treatments targeting the rejuvenation of human cardiac stem cells (hCSCs) are of great interest. In this study, the beneficial effects of human blood serum on proliferation and senescence of hCSCs have been investigated at the molecular level. We show the induction of a proliferation-related gene expression response by human blood serum at the mRNA level. The concurrent differential expression of the TGFβ target and inhibitor genes indicates the participation of TGFβ signalling in this context. Surprisingly, the application of TGFβ1 as well as the inhibition of TGFβ type I and type II receptor (TGFβRI/II) signalling strongly increased the proliferation of hCSCs. Likewise, both human blood serum and TGFβ1 reduced the senescence in hCSCs. The protective effect of serum on senescence in hCSCs was enhanced by simultaneous TGFβRI/II inhibition. These results strongly indicate a dual role of TGFβ signalling in terms of the serum-mediated effects on hCSCs. Further analysis via RNA sequencing (RNA-Seq) revealed the participation of Ras-inactivating genes wherefore a prevention of hyperproliferation upon serum-treatment in hCSCs via TGFβ signalling and Ras-induced senescence is suggested. These insights may improve treatments of heart failure in the future.
Neurodegenerative Disease: From Molecular Basis to Therapy
Authors: Ricci Claudia
Published: January 2024

Neurodegenerative diseases are a heterogeneous group of age-related disorders characterised by the progressive degeneration or death of neurons in the central or peripheral nervous system [...]
Leveraging SARS-CoV-2 Main Protease (Mpro) for COVID-19 Mitigation with Selenium-Based Inhibitors
Authors: De Luca Viviana , Angeli Andrea , Nocentini Alessio , Gratteri Paola , Pratesi Silvia , Tanini Damiano , Carginale Vincenzo , Capperucci Antonella , Supuran Claudiu T. , Capasso Clemente
Published: January 2024

The implementation of innovative approaches is crucial in an ongoing endeavor to mitigate the impact of COVID-19 pandemic. The present study examines the strategic application of the SARS-CoV-2 Main Protease (Mpro) as a prospective instrument in the repertoire to combat the virus. The cloning, expression, and purification of Mpro, which plays a critical role in the viral life cycle, through heterologous expression in Escherichia coli in a completely soluble form produced an active enzyme. The hydrolysis of a specific substrate peptide comprising a six-amino-acid sequence (TSAVLQ) linked to a p-nitroaniline (pNA) fragment together with the use of a fluorogenic substrate allowed us to determine effective inhibitors incorporating selenium moieties, such as benzoselenoates and carbamoselenoates. The new inhibitors revealed their potential to proficiently inhibit Mpro with IC50-s in the low micromolar range. Our study contributes to the development of a new class of protease inhibitors targeting Mpro, ultimately strengthening the antiviral arsenal against COVID-19 and possibly, related coronaviruses.
Serum microRNA Profiles and Pathways in Hepatitis B-Associated Hepatocellular Carcinoma: A South African Study
Authors: Sartorius Kurt , Sartorius Benn , Winkler Cheryl , Chuturgoon Anil , Shen Tsai-Wei , Zhao Yongmei , An Ping
Published: January 2024

The incidence and mortality of hepatocellular carcinoma (HCC) in Sub-Saharan Africa is projected to increase sharply by 2040 against a backdrop of limited diagnostic and therapeutic options. Two large South African-based case control studies have developed a serum-based miRNome for Hepatitis B-associated hepatocellular carcinoma (HBV-HCC), as well as identifying their gene targets and pathways. Using a combination of RNA sequencing, differential analysis and filters including a unique molecular index count (UMI) ≥ 10 and log fold change (LFC) range > 2: <−0.5 (p < 0.05), 91 dysregulated miRNAs were characterized including 30 that were upregulated and 61 were downregulated. KEGG analysis, a literature review and other bioinformatic tools identified the targeted genes and HBV-HCC pathways of the top 10 most dysregulated miRNAs. The results, which are based on differentiating miRNA expression of cases versus controls, also develop a serum-based miRNA diagnostic panel that indicates 95.9% sensitivity, 91.0% specificity and a Youden Index of 0.869. In conclusion, the results develop a comprehensive African HBV-HCC miRNome that potentially can contribute to RNA-based diagnostic and therapeutic options.
Clerodendrum chinense Stem Extract and Nanoparticles: Effects on Proliferation, Colony Formation, Apoptosis Induction, Cell Cycle Arrest, and Mitochondrial Membrane Potential in Human Breast Adenocarcinoma Breast Cancer Cells
Authors: Chittasupho Chuda , Samee Weerasak , Na Takuathung Mingkwan , Okonogi Siriporn , Nimkulrat Sathaporn , Athikomkulchai Sirivan
Published: January 2024

Breast cancer stands out as the most widespread form of cancer globally. In this study, the anticancer activities of Clerodendrum chinense (C. chinense) stem ethanolic extract were investigated. High-performance liquid chromatography (HPLC) analysis identified verbascoside and isoverbascoside as the major bioactive compounds in the C. chinense stem extract. Successfully developed nanoparticles exhibited favorable hydrodynamic diameter, polydispersity index, and surface charge, thus ensuring stability after four months of storage. The total phenolic content and total flavonoid contents in the nanoparticles were reported as 88.62% and 95.26%, respectively. The C. chinense stem extract demonstrated a dose-dependent inhibitory effect on MCF-7, HeLa, A549, and SKOV-3 cancer cell lines, with IC50 values of 109.2, 155.6, 206.9, and 423 µg/mL, respectively. C. chinense extract and NPs exhibited dose-dependent cytotoxicity and the highest selectivity index values against MCF-7 cells. A dose-dependent reduction in the colony formation of MCF-7 cells was observed following treatment with the extract and nanoparticles. The extract induced cytotoxicity in MCF-7 cells through apoptosis and necrosis. C. chinense stem extract and nanoparticles decreased mitochondrial membrane potential (MMP) and induced G0/G1 phase arrest in MCF-7 cells. In conclusion, use of C. chinense stem extract and nanoparticles may serve as a potential therapeutic approach for breast cancer, thus warranting further exploration.
Gap Junction Channel Regulation: A Tale of Two Gates—Voltage Sensitivity of the Chemical Gate and Chemical Sensitivity of the Fast Voltage Gate
Authors: Peracchia Camillo
Published: January 2024

Gap junction channels are regulated by gates sensitive to cytosolic acidification and trans-junctional voltage (Vj). We propose that the chemical gate is a calmodulin (CaM) lobe. The fast-Vj gate is made primarily by the connexin’s NH2-terminus domain (NT). The chemical gate closes the channel slowly and completely, while the fast-Vj gate closes the channel rapidly but incompletely. The chemical gate closes with increased cytosolic calcium concentration [Ca2+]i and with Vj gradients at Vj’s negative side. In contrast, the fast-Vj gate closes at the positive or negative side of Vj depending on the connexin (Cx) type. Cxs with positively charged NT close at Vj’s negative side, while those with negatively charged NT close at Vj’s positive side. Cytosolic acidification alters in opposite ways the sensitivity of the fast-Vj gate: it increases the Vj sensitivity of negative gaters and decreases that of positive gaters. While the fast-Vj gate closes and opens instantaneously, the chemical gate often shows fluctuations, likely to reflect the shifting of the gate (CaM’s N-lobe) in and out of the channel’s pore.
Structural Insights into the Penicillin-Binding Protein 4 (DacB) from Mycobacterium tuberculosis
Authors: Kang Sung-Min , Kim Do-Hee
Published: January 2024

Mycobacterium tuberculosis, a major cause of mortality from a single infectious agent, possesses a remarkable mycobacterial cell envelope. Penicillin-Binding Proteins (PBPs) are a family of bacterial enzymes involved in the biosynthesis of peptidoglycan. PBP4 (DacB) from M. tuberculosis (MtbPBP4) has been known to function as a carboxypeptidase, and the role and significance of carboxypeptidases as targets for anti-tuberculosis drugs or antibiotics have been extensively investigated over the past decade. However, their precise involvement remains incompletely understood. In this study, we employed predictive modeling and analyzed the three-dimensional structure of MtbPBP4. Interestingly, MtbPBP4 displayed a distinct domain structure compared to its homologs. Docking studies with meropenem verified the presence of active site residues conserved in PBPs. These findings establish a structural foundation for comprehending the molecular function of MtbPBP4 and offer a platform for the exploration of novel antibiotics.
Chemical Composition of Methanol Extracts from Leaves and Flowers of Anemonopsis macrophylla (Ranunculaceae)
Authors: Kostikova Vera A. , Petrova Natalia V. , Chernonosov Alexander A. , Koval Vladimir V. , Kovaleva Evgeniia R. , Wang Wei , Erst Andrey S.
Published: January 2024

Anemonopsis Siebold et Zucc. is an unstudied single-species genus belonging to the tribe Cimicifugeae (Ranunculaceae). The only species of this genus—Anemonopsis macrophylla Siebold and Zucc.—is endemic to Japan. There are no data on its chemical composition. This work is the first to determine (with liquid chromatography–high-resolution mass spectrometry, LC-HRMS) the chemical composition of methanol extracts of leaves and flowers of A. macrophylla. More than 100 compounds were identified. In this plant, the classes of substances are coumarins (13 compounds), furocoumarins (3), furochromones (2), phenolic acids (21), flavonoids (27), and fatty acids and their derivatives (15 compounds). Isoferulic acid (detected in extracts from this plant) brings this species closer to plants of the genus Cimicifuga, one of the few genera containing this acid and ferulic acid at the same time. Isoferulic acid is regarded as a reference component of a quality indicator of Cimicifuga raw materials. The determined profiles of substances are identical between the leaf and flower methanol extracts. Differences in levels of some identified substances were revealed between the leaf and flower extracts of A. macrophylla; these differences may have a substantial impact on the manifestation of the biological and pharmacological effects of the extracts in question.
Advancing Antimicrobial Textiles: A Comprehensive Study on Combating ESKAPE Pathogens and Ensuring User Safety
Authors: Vojnits Kinga , Mohseni Majid , Parvinzadeh Gashti Mazeyar , Nadaraja Anupama Vijaya , Karimianghadim Ramin , Crowther Ben , Field Brad , Golovin Kevin , Pakpour Sepideh
Published: January 2024

Antibiotic-resistant bacteria, ESKAPE pathogens, present a significant and alarming threat to public health and healthcare systems. This study addresses the urgent need to combat antimicrobial resistance by exploring alternative ways to reduce the health and cost implications of infections caused by these pathogens. To disrupt their transmission, integrating antimicrobial textiles into personal protective equipment (PPE) is an encouraging avenue. Nevertheless, ensuring the effectiveness and safety of these textiles remains a persistent challenge. To achieve this, we conduct a comprehensive study that systematically compares the effectiveness and potential toxicity of five commonly used antimicrobial agents. To guide decision making, a MULTIMOORA method is employed to select and rank the optimal antimicrobial textile finishes. Through this approach, we determine that silver nitrate is the most suitable choice, while a methoxy-terminated quaternary ammonium compound is deemed less favorable in meeting the desired criteria. The findings of this study offer valuable insights and guidelines for the development of antimicrobial textiles that effectively address the requirements of effectiveness, safety, and durability. Implementing these research outcomes within the textile industry can significantly enhance protection against microbial infections, contribute to the improvement of public health, and mitigate the spread of infectious diseases.
Application of New Filling Material Based on Combined Heat and Power Waste for Sewage Treatment in Constructed Wetlands
Authors: Malinowski Paweł , Dąbrowski Wojciech , Karolinczak Beata
Published: January 2024

The filling of constructed wetlands (CWs) affects the efficiency of sewage treatment and proper operation. Mineral aggregates are most often used as filling materials. Significant environmental burdens from mineral mining operations justify the search for waste fill. This study aimed to determine the possibility of increasing the efficiency of CW by using a Certyd aggregate as a new filling. Certyd is produced in the sintering process of coal ash, a waste from combined heat and power (CHP) plant operation. Comprehensive two-year studies were conducted using two real-scale subsurface vertical flow (SS VF) CWs supplied with domestic sewage. One bed was filled with a Certyd and the other was filled with appropriate fractions of a mineral aggregate. Both beds worked in parallel, and to compare their effectiveness, account seasonality was taken into account. The SS-VF Certyd-filled bed achieved an average efficiency of 88.0% for biological oxygen demand (BOD5), 80.2% for chemical oxygen demand (COD), 80.4% for suspended solids (SSs), 80.2 for ammonia nitrogen (N-NH4), 72.2% for total nitrogen (TN), and 55.3% for total phosphorus (TP), while the gravel-filled bed achieved 84.5%, 77.0%, 86.9%, 74.2%, 69.4%, and 57.8% for the whole research period, respectively. A higher effect of the removed unit load was achieved in the bed filled with Certyd (36.2 g BOD5 m−2 d−1, 50.0 g COD m−2 d−1, 5.88 g SS m−2 d−1, 7.1 g TN m−2 d−1, 7.9 g N-NH4 m−2 d−1, 0.79 g TP m−2 d−1) compared to the gravel-filled bed (34.7 g BOD5 m−2 d−1, 47.0 g COD, 6.35 g SS m−2 d−1, 6.9 g TN m−2 d−1, 7.3 g m−2 d−1 N-NH4, 0.83 g TP m−2 d−1).
Effects of Direct and Pulse Plating on the Co-Deposition of Sn–Ni/TiO2 Composite Coatings
Authors: Rosolymou Eleni , Karantonis Antonis , Pavlatou Evangelia A.
Published: January 2024

Sn–Ni alloy matrix coatings co-deposited with TiO2 nanoparticles (Evonik P25) were produced utilizing direct (DC) and pulse electrodeposition (PC) from a tin–nickel chloride-fluoride electrolyte with a loading of TiO2 nanoparticles equal to 20 g/L. The structural and morphological characteristics of the resultant composite coatings were correlated with the compositional modifications that occurred within the alloy matrix and expressed via a) TiO2 co-deposition rate and b) composition of the matrix; this was due to the application of different current types (DC or PC electrodeposition), and different current density values. The results demonstrated that under DC electrodeposition, the current density exhibited a more significant impact on the composition of the alloy matrix than on the incorporation rate of the TiO2 nanoparticles. Additionally, PC electrodeposition favored the incorporation rate of TiO2 nanoparticles only when applying a low peak current density (Jp = 1 Adm−2). All of the composite coatings exhibited the characteristic cauliflower-like structure, and were characterized as nano-crystalline. The composites’ surface roughness demonstrated a significant influence from the TiO2 incorporation rate. However, in terms of microhardness, higher co-deposition rates of embedded TiO2 nanoparticles within the alloy matrix were associated with decreased microhardness values. The best wear performance was achieved for the composite produced utilizing DC electrodeposition at J = 1 Adm−2, which also demonstrated the best photocatalytic behavior under UV irradiation. The corrosion study of the composite coatings revealed that they exhibit passivation, even at elevated anodic potentials.
Evaluation of True Bonding Strength for Adhesive Bonded Carbon Fiber-Reinforced Plastics
Authors: Takamura Maruri , Isozaki Minori , Takeda Shinichi , Oya Yutaka , Koyanagi Jun
Published: January 2024

Carbon fiber-reinforced thermoplastics (CFRTPs) have attracted attention in aerospace because of their superior specific strength and stiffness. It can be assembled by adhesive bonding; however, the existing evaluation of bonding strength is inadequate. For example, in a single-lap shear test, the weld zone fails in a combined stress state because of the bending moment. Therefore, the strength obtained experimentally is only the apparent strength. The true bonding strength was obtained via numerical analysis by outputting the local stress state at the initiation point of failure. In this study, the apparent and true bonding strengths were compared with respect to three types of strength evaluation tests to comprehensively evaluate bonding strength. Consequently, the single-lap shear test underestimates the apparent bonding strength by less than 14% of the true bonding strength. This indicates that care should be taken when determining the adhesion properties for use in numerical analyses based on experimental results. We also discussed the thickness dependence of the adhesive on the stress state and found that the developed shear test by compression reduced the discrepancy between apparent and true strength compared with the single-lap shear test and reduced the thickness dependence compared with the flatwise tensile test.
Research on Deep Q-Network Hybridization with Extended Kalman Filter in Maneuvering Decision of Unmanned Combat Aerial Vehicles
Authors: Ruan Juntao , Qin Yi , Wang Fei , Huang Jianjun , Wang Fujie , Guo Fang , Hu Yaohua
Published: January 2024

To adapt to the development trend of intelligent air combat, it is necessary to research the autonomous generation of maneuvering decisions for unmanned combat aerial vehicles (UCAV). This paper presents a maneuver decision-making method for UCAV based on a hybridization of deep Q-network (DQN) and extended Kalman filtering (EKF). Firstly, a three-dimensional air combat simulation environment is constructed, and a flight motion model of UCAV is designed to meet the requirements of the simulation environment. Secondly, we evaluate the current situation of UCAV based on their state variables in air combat, for further network learning and training to obtain the optimal maneuver strategy. Finally, based on the DQN, the system state equation is constructed using the uncertain parameter values of the current network, and the observation equation of the system is constructed using the parameters of the target network. The optimal parameter estimation value of the DQN is obtained by iteratively updating the solution through EKF. Simulation experiments have shown that this autonomous maneuver decision-making method hybridizing DQN with EKF is effective and reliable, as it can eliminate the opponent and preserve its side.
Circadian Rhythm Perturbation Aggravates Gut Microbiota Dysbiosis in Dextran Sulfate Sodium-Induced Colitis in Mice
Authors: Amara Joseph , Itani Tarek , Hajal Joelle , Bakhos Jules-Joel , Saliba Youakim , Aboushanab Saied A. , Kovaleva Elena G. , Fares Nassim , Mondragon Alicia C. , Miranda Jose Manuel
Published: January 2024

Circadian rhythm disruption is increasingly considered an environmental risk factor for the development and exacerbation of inflammatory bowel disease. We have reported in a previous study that nychthemeral dysregulation is associated with an increase in intestinal barrier permeability and inflammation in mice with dextran sulfate sodium (DSS)-induced colitis. To investigate the effect of circadian rhythm disruption on the composition and diversity of the gut microbiota (GM), sixty male C57BL/6J mice were initially divided to two groups, with the shifted group (n = 30) exposed to circadian shifts for three months and the non-shifted group (n = 30) kept under a normal light–dark cycle. The mice of the shifted group were cyclically housed for five days under the normal 12:12 h light–dark cycle, followed by another five days under a reversed light–dark cycle. At the end of the three months, a colitis was induced by 2% DSS given in the drinking water of 30 mice. Animals were then divided into four groups (n = 15 per group): sham group non-shifted (Sham-NS), sham group shifted (Sham-S), DSS non-shifted (DSS-NS) and DSS shifted (DSS-S). Fecal samples were collected from rectal content to investigate changes in GM composition via DNA extraction, followed by high-throughput sequencing of the bacterial 16S rRNA gene. The mouse GM was dominated by three phyla: Firmicutes, Bacteroidetes and Actinobacteria. The Firmicutes/Bacteroidetes ratio decreased in mice with induced colitis. The richness and diversity of the GM were reduced in the colitis group, especially in the group with inverted circadian rhythm. Moreover, the GM composition was modified in the inverted circadian rhythm group, with an increase in Alloprevotella, Turicibacter, Bacteroides and Streptococcus genera. Circadian rhythm inversion exacerbates GM dysbiosis to a less rich and diversified extent in a DSS-induced colitis model. These findings show possible interplay between circadian rhythm disruption, GM dynamics and colitis pathogenesis.