bluuebunny/crossref_metadata_2025 huggingface dataset:
Interventions for Perinatal Depression and Anxiety in Fathers: A Mini-Review
Authors: Rodrigues Andre L. , Ericksen Jennifer , Watson Brittany , Gemmill Alan W. , Milgrom Jeannette
Published: January 2022

Background and Objectives

Up to 10% of fathers experience perinatal depression, often accompanied by anxiety, with a detrimental impact on the emotional and behavioural development of infants. Yet, few evidence-based interventions specifically for paternal perinatal depression or anxiety exist, and few depressed or anxious fathers engage with support. This mini-review aims to build on the evidence base set by other recent systematic reviews by synthesising more recently available studies on interventions for paternal perinatal depression and anxiety. Secondarily, we also aimed to identify useful information on key implementation strategies, if any, that increase the engagement of men.

Methods

We drew upon three major previous systematic reviews and performed an updated search of PubMed/Medline; Psycinfo; Cochrane Database; Embase and Cinahl. The search was limited to trials, feasibility studies or pilot studies of interventions published between 2015 and 2020 that reported on fathers' perinatal mental health. We included psychological, educational, psychosocial, paternal, couple-focused, or group therapies, delivered face-to-face, via telephone and/or online that reported on either paternal depression, anxiety or both.

Results

Eleven studies satisfied search criteria (5 of which were not included in previous reviews). The majority were randomised controlled trials. Most interventions incorporated counselling, therapy or psychoeducation and took an indirect approach to perinatal mental health through antenatal or postnatal education and were couple-focused. No studies reported a presence of diagnosed depression or anxiety at baseline, although five studies reported a positive effect on sub-threshold symptoms.

Discussion

There was some evidence that these approaches may be useful in the initial engagement of fathers with perinatal supports and improve depression and anxiety scores. No studies targeted the explicit treatment of clinically depressed or anxious men, and this remains the most substantial gap in the peer-reviewed evidence base. Our results highlight the need to deliver perinatal interventions specifically designed for men and evaluate them in populations with clinical levels of depressive and anxious symptomatology.
From Habit-Forming to Habit-Breaking Availability: Experiences on Electronic Gambling Machine Closures During COVID-19
Authors: Marionneau Virve , Järvinen-Tassopoulos Johanna
Published: January 2022

Electronic gambling machines (EGMs) are among the most harmful forms of gambling. The structural characteristics of EGMs prolong and reinforce gambling similarly to other habit-forming technologies. In Finland, the wide availability of EGMs in non-casino locations is likely to further reinforce the habit-creating nature of gambling offer by incorporating EGMs into everyday practices. The COVID-19 pandemic changed the landscape of gambling in Finland. The most visible change was the closure of land-based EGMs in non-casino environments, arcades, and the casino in March 2020. Since then, the status of EGMs has varied depending on the pandemic situation. The current qualitative study focuses on how Finnish past-year gamblers experience prolonged EGM closures and occasional re-openings 1 year into the pandemic. The data consist of responses to an online questionnaire eliciting experiences (N = 187) as well as interviews (N = 27, conducted in groups or alone). To aid our analysis, we employ the sociological pragmatist theory of the concept of “habit.” The analysis focuses on gambler experiences on EGM shutdowns and re-openings, and views on whether closures have contributed to abstaining from gambling or to shifting to other gambling products. Policy implications of the results are discussed.
Factors Affecting Online Chinese as a Foreign Language Learning Stickiness: A Study on International Students in China
Authors: Chen Chen
Published: January 2022

This study explored international students’ online Chinese as a foreign language learning stickiness in a Chinese university context. A new theoretical model was designed and verified to serve the mixed-method investigation. Participants were a group of 194 international students learning CFL online in a Chinese university. Data were collected through an online questionnaire for all the students and semi-structural individual interviews with eight volunteer participants. The structural equation modeling was conducted to analyze the collected quantitative data, and content analysis was used for the qualitative interview. Findings revealed that the online learning stickiness was significantly impacted by students’ learning expectancy confirmation, which was closely correlated with three key factors: academic integration, social integration, and technological factors. Moreover, social integration was found to be a direct contributor to learning stickiness. These results highlighted the importance of the high quality of the curriculum, the harmonious learning atmosphere, and the need for technical preparations and training for online Chinese language teaching and learning. The study also emphasized the need of integrating social interaction into Chinese learning in an online context. Investigation of a wider range of Chinese learners was recommended for future studies on learning stickiness and the new online approach.
A Study on the Correlation Between Media Usage Frequency and Audiences’ Risk Perception, Emotion and Behavior
Authors: Li Peng-Peng , Zhong Fangqi
Published: January 2022

Whether risk events can be effectively controlled and mitigated is largely influenced by people’s perceptions of risk events and their behavioral cooperation. Therefore, this study used a web-based questionnaire (N = 306) to investigate the specific factors influencing people’s risk perceptions and behaviors, and included a test for the difference in the effect of positive and negative emotions of the audiences. The results show that the overall model has good explanatory power (R2 = 61%) for the behavioral variables, and (1) how people’s use of different media (especially TV and online media) significantly influenced their positive and negative emotions; (2) how people’s frequency of TV use significantly influenced their risk susceptibility and how online media use significantly influenced their risk severity (with some differences in people’s perceptions of efficacy between different media); (3) how people’s sense of efficacy for risky events is the strongest predictor of their risk control behavior; and (4) that there are different mediating effects of different emotions and risk severity and sense of efficacy between the frequency of media use and risk control behavior.
Psychological Capital Profiles and Their Relationship With Internal Learning in Teams of Undergraduate Students
Authors: Geremias Rosa Lutete , Lopes Miguel Pereira , Soares André Escórcio
Published: January 2022

This study aims to analyze the relationship between psychological capital profiles and internal learning in teams. The participants in this study were 480 undergraduate students. We performed a cluster analysis using the SPSS and yielded four distinct psychological capital profiles. The student profile with the highest scores in self-efficacy, optimism, hope, and resilience (Profile 2-Fully PsyCap) exhibited also the highest scores of internal learning in teams. On the other hand, the student profile with the lowest scores in self-efficacy, optimism, hope, and resilience (Profile 1- Empty PsyCap) presented the lowest scores of internal learning in teams. It is also noteworthy that there was no significant relationship between the profile with a positive combination between self-efficacy and hope (profile 4) and the profile that presents the optimism as the only positive psychological capability (profile 3), in the way they relate to internal learning in teams, which led us to reject the second hypothesis of the study. This study reinforces the role of psychological capital in academic settings and suggests that psychological capital profiles can affect internal learning in teams differentially.
How to Reduce Mental Health Burden in Health Care Workers During COVID-19?–A Scoping Review of Guideline Recommendations
Authors: Halms Theresa , Strasser Martina , Kunz Miriam , Hasan Alkomiet
Published: January 2022

The COVID-19 pandemic has posed an unprecedented demand and a huge burden for healthcare workers (HCWs) worldwide, with alarming reports of heightened mental health problems. To counteract these mental health challenges, guidelines and recommendations for the support of HCWs during the COVID-19 pandemic have been published. With this scoping review and guideline evaluation, we aim to provide a critical overview of these guidelines and recommendations and to guide policy makers in establishing respective surveillance and care programs. In summary, 41 articles were included in this review which were published between April 2020 and May 2021. Across all articles, the guidelines and recommendations could be clustered into four main categories: “Social/structural support,” “Work environment,” “Communication/Information,” “Mental health support.” Although there was substantial agreement across articles about the recommendations given, empirical evidence on the effectiveness of these recommendations is still lacking. Moreover, most recommendations were developed without involving different members of the target group (HCWs) or other involved stakeholders. Strategies to detect potential barriers and to implement these guidelines in clinical practice are lacking.
The Need for Psychedelic-Assisted Therapy in the Black Community and the Burdens of Its Provision
Authors: Smith Darron T. , Faber Sonya C. , Buchanan NiCole T. , Foster Dale , Green Lilith
Published: January 2022

Psychedelic medicine is an emerging field that examines entheogens, psychoactive substances that produce non-ordinary states of consciousness (NOSC). 3,4-methylenedioxymethamphetamine (MDMA) is currently in phase-3 FDA clinical trials in the United States (US) and Canada to treat the symptoms of posttraumatic stress disorder (PTSD). MDMA is used in conjunction with manualized therapy, because of its effectiveness in reducing fear-driven stimuli that contribute to trauma and anxiety symptoms. In 2017, the FDA designated MDMA as a “breakthrough therapy,” signaling that it has advantages in safety, efficacy, and compliance over available medication for the treatment of trauma-, stress-, and anxiety-related disorders such as PTSD. In the US and Canada, historical and contemporary racial mistreatment is frequently experienced by Black people via a variety of macro and micro insults. Such experiences trigger physiological responses of anxiety and fear, which are associated with chronically elevated stress hormone levels (e.g., cortisol and epinephrine), similar to levels documented among those diagnosed with an anxiety disorder. This paper will explore the benefits of entheogens within psychedelic assisted-therapy and their potential benefits in addressing the sequelae of pervasive and frequent negative race-based experiences and promoting healing and thriving among Black, Indigenous and other People of Color (BIPOC). The author(s) discuss the ethical responsibility for providing psychedelic-assisted therapy within a culturally competent provider framework and the importance of psychedelic researchers to recruit and retain BIPOC populations in research and clinical training.
Personality and Peripartum Changes in Perceived Social Support: Findings From Two Prospective-Longitudinal Studies in (Expectant) Mothers and Fathers
Authors: Asselmann Eva , Garthus-Niegel Susan , Martini Julia
Published: January 2022

Objective

The aim of this study was to examine changes in perceived social support from early pregnancy to 2 years postpartum and to test whether these changes (a) differ between mothers and fathers or (b) vary as a function of the Big Five personality traits.

Background

Higher peripartum social support in (expectant) mothers and fathers has been associated with fewer complications during pregnancy and delivery as well as better parental and offspring health.

Methods

Prospective-longitudinal data from two regional-epidemiological samples from Germany were used: MARI (N = 396, including n = 293 mothers and n = 103 fathers) and DREAM (N = 2,819, including n = 1,689 mothers and n = 1,130 fathers). The Big Five personality traits were assessed during pregnancy in MARI as well as 8 weeks after the anticipated birth date in DREAM with short forms of the Big Five Inventory. Perceived social support was assessed during pregnancy, 4 months postpartum, and 16 months postpartum in MARI as well as during pregnancy, 14 months postpartum, and 2 years postpartum in DREAM using the short version of the Social Support Questionnaire.

Results

Multilevel analyses revealed that perceived social support decreased across the peripartum period, and this decrease did not differ between mothers and fathers. More extraverted, emotionally stable, agreeable, conscientious, and open parents perceived higher levels of social support across the peripartum period. The peripartum decrease of perceived social support was smaller in parents who were more extraverted.

Conclusion

Our findings suggest that especially extraversion plays an important role for high and stable levels of perceived social support across the peripartum period.

Implications

Particularly highly introverted parents might profit from targeted social support interventions.
Implementation of DHIS2 for Disease Surveillance in Guinea: 2015–2020
Authors: Reynolds Eileen , Martel Lise D. , Bah Mamadou Oury , Bah Marlyatou , Bah Mariama Boubacar , Boubacar Barry , Camara Nouhan , Camara Yero Boye , Corvil Salomon , Diallo Boubacar Ibrahima , Diallo Ibrahima Telly , Diallo Mamadou Kadiatou , Diallo Mamadou Tafsir , Diallo Telly , Guilavogui Siba , Hemingway-Foday Jennifer J. , Hann Fatoumata , Kaba Abdoulaye , Kaba Almamy Karamokoba , Kande Mohamed , Lamarana Diallo Mamadou , Middleton Kathy , Sidibe N'valy , Souare Ousmane , Standley Claire J. , Stolka Kristen B. , Tchwenko Samuel , Worrell Mary Claire , MacDonald Pia D. M.
Published: January 2022

A robust epidemic-prone disease surveillance system is a critical component of public health infrastructure and supports compliance with the International Health Regulations (IHR). One digital health platform that has been implemented in numerous low- and middle-income countries is the District Health Information System Version 2 (DHIS2). In 2015, in the wake of the Ebola epidemic, the Ministry of Health in Guinea established a strategic plan to strengthen its surveillance system, including adoption of DHIS2 as a health information system that could also capture surveillance data. In 2017, the DHIS2 platform for disease surveillance was piloted in two regions, with the aim of ensuring the timely availability of quality surveillance data for better prevention, detection, and response to epidemic-prone diseases. The success of the pilot prompted the national roll-out of DHIS2 for weekly aggregate disease surveillance starting in January 2018. In 2019, the country started to also use the DHIS2 Tracker to capture individual cases of epidemic-prone diseases. As of February 2020, for aggregate data, the national average timeliness of reporting was 72.2%, and average completeness 98.5%; however, the proportion of individual case reports filed was overall low and varied widely between diseases. While substantial progress has been made in implementation of DHIS2 in Guinea for use in surveillance of epidemic-prone diseases, much remains to be done to ensure long-term sustainability of the system. This paper describes the implementation and outcomes of DHIS2 as a digital health platform for disease surveillance in Guinea between 2015 and early 2020, highlighting lessons learned and recommendations related to the processes of planning and adoption, pilot testing in two regions, and scale up to national level.
Bibliometric Analysis of International Scientific Production on Pharmacologic Treatments for SARS-CoV-2/COVID-19 During 2020
Authors: Ruiz-Fresneda Miguel A. , Jiménez-Contreras Evaristo , Ruiz-Fresneda Carlos , Ruiz-Pérez Rafael
Published: January 2022

Background

COVID-19 is causing a grave global health and economic crisis and the fight against the pandemic has led to unprecedented scientific activity. Bibliometrics could be a useful tool for guiding future researches lines and promoting international collaboration for an effective treatment. For this purpose, we have conducted a bibliometric analysis of scientific publications on drugs and therapies used to treat COVID-19 during 2020.

Methods

Data source: Web of Science. We gathered data on scientific production relating to drugs used to treat COVID-19. We calculated impact factors and analyzed production by institution, country, and journal, visualizing our results in bibliometric networks.

Results

In 1 year, production relating to COVID-19 exceeded 100 000 publications, with over 6,500 on Drugs and COVID-19. Research into hydroxychloroquine and chloroquine, remdesivir, lopinavir and ritonavir, tocilizumab and convalescent plasma is particularly noteworthy. Mean citations/study range from 11.9 to 15.4. Producer institutions fall into three groups: one in the US and centered on Harvard Medical School; another in Europe led by INSERS; and another in China led by Huazhong University of Science and Technology. Production by journal is widespread but the Journal of Medical Virology, International Journal of Antimicrobial Agents, and American Journal of Transplantation are noteworthy.

Conclusions

The volume of research that is currently under way is comparable to the magnitude of the pandemic itself. Such a high volume of studies is infrequent and the impact they have achieved has no known precedent. The producing countries are those with highest incidence of the pandemic and greatest scientific potential; moreover, inter-agency and international collaboration has reached extraordinarily high levels.
Impact of Environmental Degradation on Human Health: An Assessment Using Multicriteria Decision Making
Authors: Mumtaz Ayesha , Rehman Erum , Rehman Shazia , Hussain Iftikhar
Published: January 2022

Air pollution has emerged as a major global concern in recent decades as a result of rapid urbanization and industrialization, leading to a variety of adverse health outcomes. This research aims to investigate the influence of exposure to ambient and household particulate matter pollution (PM2.5), and ground-level ozone (O3) pollution on respiratory and cardiac mortality in Pakistan. We used grey incidence analysis (GIA) methodology to estimate the degree of proximity among selected variables and rank them based on mortality. Hurwicz's criterion is then adopted for further optimization by prioritizing the selected factors with the greatest influence on respiratory and cardiac mortality. The GIA findings revealed that asthma mortality is considerably impacted by exposure to ambient and household PM2.5 concentration while ischemic heart disease (IHD) mortality is potentially influenced by ground-level ozone exposure. Furthermore, results based on Hurwicz's analysis demonstrated that exposure to ambient PM2.5 concentration appeared as the most intensified factor of respiratory and cardiac mortality. This corroboration adds to the growing body of research demonstrating that exposure to ambient PM2.5 adversely leads to respiratory and cardiac risks, emphasizing the demand for further improvement of air quality in Pakistan. Besides, the suggested methodologies provide a valuable tool and additional practical knowledge for policymakers and decision-makers in drawing rational decisions.
A User’s Guide to the Magnetically Connected Space Weather System: A Brief Review
Authors: Beedle Jason M. H. , Rura Christopher E. , Simpson David G. , Cohen Hale I. , Moraes Filho Valmir P. , Uritsky Vadim M.
Published: January 2022

This article provides a concise review of the main physical structures and processes involved in space weather’s interconnected systems, emphasizing the critical roles played by magnetic topology and connectivity. The review covers solar drivers of space weather activity, the heliospheric environment, and the magnetospheric response, and is intended to address a growing cross-disciplinary audience interested in applied aspects of modern space weather research and forecasting. The review paper includes fundamental facts about the structure of space weather subsystems and special attention is paid to extreme space weather events associated with major solar flares, large coronal mass ejections, solar energetic particle events, and intense geomagnetic perturbations and their ionospheric footprints. This paper aims to be a first step towards understanding the magnetically connected space weather system for individuals new to the field of space weather who are interested in the basics of the space weather system and how it affects our daily lives.
Gas-Phase Reactivity of OH Radicals With Ammonia (NH3) and Methylamine (CH3NH2) at Around 22 K
Authors: González Daniel , Ballesteros Bernabé , Canosa André , Albaladejo José , Jiménez Elena
Published: January 2022

Interstellar molecules containing N atoms, such as ammonia (NH3) and methylamine (CH3NH2), could be potential precursors of amino acids like the simplest one, glycine (NH2CH2COOH). The gas-phase reactivity of these N-bearing species with OH radicals, ubiquitous in the interstellar medium, is not known at temperatures of cold dark molecular clouds. In this work, we present the first kinetic study of these OH-reactions at around 22 K and different gas densities [(3.4–16.7) × 1016 cm−3] in helium. The obtained rate coefficients, with ± 2σ uncertainties, can be included in pure gas-phase or gas-grain astrochemical models to interpret the observed abundances of NH3and CH3NH2. We observed an increase ofk1andk2with respect to those previously measured by others at the lowest temperatures for which rate coefficients are presently available: 230 and 299 K, respectively. This increase is about 380 times for NH3and 20 times for CH3NH2. Although the OH + NH3reaction is included in astrochemical kinetic databases, the recommended temperature dependence fork1is based on kinetic studies at temperatures above 200 K. However, the OH + CH3NH2reaction is not included in astrochemical networks. The observed increase ink1at ca. 22 K does not significantly change the abundance of NH3in a typical cold dark interstellar cloud. However, the inclusion ofk2at ca. 22 K, not considered in astrochemical networks, indicates that the contribution of this destruction route for CH3NH2is not negligible, accounting for 1/3 of the assumed main depletion route (reaction with HCO+) in this IS environment.

k1(OH+NH3)=(2.7±0.1)×10−11cm3s-1k2(OH+CH3NH2)=(3.9±0.1)×10−10cm3s-1
Molecular Characterization, Tissue Distribution and Differential Nutritional Regulation of Three n-3 LC-PUFA Biosynthesis-Related Genes in Hybrid Grouper (Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂)
Authors: Wu Qingjun , Zheng Zhi , Wang Chuijin , Wang Yao , Sun Yuejia , Gao Yujie
Published: January 2022

Elongases of very long-chain fatty acids (Elovls) and fatty acid desaturases (Fads) are crucial enzymes involved in the biosynthesis of long-chain polyunsaturated fatty acids (LC-PUFAs). In this paper, we report the molecular cloning and characterization of three genes from the marine teleost Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂, and analyzed tissue distribution and their expression in response to dietary n-3 LC-PUFA levels after a 42-day feeding experiment. The elovl5, elovl8 and fads2 genes encoded 294, 263 and 445 amino acids, respectively, which exhibited all the characteristics of the Elovl and Fads family. Tissue distribution analysis revealed that elovl5, elovl8 and fads2 were widely transcribed in various tissues, with the highest level in the brain, as described in other carnivorous marine teleosts. The transcript levels of elovl5, elovl8 and fads2 in the liver were significantly affected by dietary n-3 LC-PUFA, and higher LC-PUFA levels repressed their expression. These results demonstrated, for the first time, the presence and nutritional modulation of elovl5, elovl8 and fads2 cDNA in the juvenile hybrid grouper. Further studies are needed to determine the functional characterization of these genes and explore the mechanism of these genes when regulated by dietary fatty lipid profiles in this species.
Laboratory Performance Evaluation of Novel Bituminous Coal Pellet Combustion in an Automatic Heating Stove
Authors: Jiao Mingze , Yan Ming , Liu Yafei , Mawusi Sylvester K. , Shrestha Prabin , Xue Chunyu , Song Hua , Wang Haimiao , Liu Zhongpan , Xu Yi , Liu Guangqing
Published: January 2022

Coal is China’s dominant energy source, among which bituminous coal is the most extensive and plentiful. Using bituminous coal resources to design a low-emission household fuel is very important for rural poverty areas. In this work, a new type of bituminous coal pellet (BCP) fuel using an automatic prototype pellet stove was designed for the first time. This study mainly shows the emission characteristic results of BCPs and some comparisons with other commercial solid fuels. Fuel property, PM2.5 morphology, and ash characteristics of the novel fuel were also assessed. In terms of fuel properties, BCPs had a cold compressive strength of 637.2 N, a heating value of 22.26 MJ/kg, and many fine pores in the cross-section for air entry. The real-time emissions of BCPs were stable during combustion. The emission factors of PM2.5 and CO of BCPs were 1.36–2.29 g/kg and 11.1–18.0 g/kg, which were significantly lower than those of bituminous chunk and bituminous briquette (p < 0.05). PM2.5 and CO reduced emissions by 83–90% and 61–76%, respectively, compared with raw coal chunk. According to the chemical composition and morphological characteristics, the PM2.5 from BCP combustion can be divided into fine particulates, molten char particles, and char fragmentation. The ash from BCPs had a higher melting temperature (over 1300 °C), with removable ash agglomeration. Overall, the results presented in this study highlight that turning bituminous coal into pellets and burning them in automatic stoves could noticeably reduce PM2.5 and CO emissions, effectively improving rural air quality.
KasQ an Epimerase Primes the Biosynthesis of Aminoglycoside Antibiotic Kasugamycin and KasF/H Acetyltransferases Inactivate Its Activity
Authors: Rattinam Rajesh , Basha R. Sidick , Wang Yung-Lin , Wang Zhe-Chong , Hsu Ning-Shian , Lin Kuan-Hung , Zadeh Saeid Malek , Adhikari Kamal , Lin Jin-Ping , Li Tsung-Lin
Published: January 2022

Kasugamycin (KSM), an aminoglycoside antibiotic, is composed of three chemical moieties: D-chiro-inositol, kasugamine and glycine imine. Despite being discovered more than 50 years ago, the biosynthetic pathway of KSM remains an unresolved puzzle. Here we report a structural and functional analysis for an epimerase, KasQ, that primes KSM biosynthesis rather than the previously proposed KasF/H, which instead acts as an acetyltransferase, inactivating KSM. Our biochemical and biophysical analysis determined that KasQ converts UDP-GlcNAc to UDP-ManNAc as the initial step in the biosynthetic pathway. The isotope-feeding study further confirmed that 13C, 15N-glucosamine/UDP-GlcNH2 rather than glucose/UDP-Glc serves as the direct precursor for the formation of KSM. Both KasF and KasH were proposed, respectively, converting UDP-GlcNH2 and KSM to UDP-GlcNAc and 2-N’-acetyl KSM. Experimentally, KasF is unable to do so; both KasF and KasH are instead KSM-modifying enzymes, while the latter is more specific and reactive than the former in terms of the extent of resistance. The information gained here lays the foundation for mapping out the complete KSM biosynthetic pathway.
Challenges and Advances in Chimeric Antigen Receptor Therapy for Acute Myeloid Leukemia
Authors: Marvin-Peek Jennifer , Savani Bipin N. , Olalekan Oluwole O. , Dholaria Bhagirathbhai
Published: January 2022

The advent of chimeric antigen receptor (CAR) T-cell therapy has led to dramatic remission rates in multiple relapsed/refractory hematologic malignancies. While CAR T-cell therapy has been particularly successful as a treatment for B-cell malignancies, effectively treating acute myeloid leukemia (AML) with CARs has posed a larger challenge. AML not only creates an immunosuppressive tumor microenvironment that dampens CAR T-cell responses, but it also lacks many unique tumor-associated antigens, making leukemic-specific targeting difficult. One advantage of CAR T-cell therapy compared to alternative treatment options is the ability to provide prolonged antigen-specific immune effector and surveillance functions. Since many AML CAR targets under investigation including CD33, CD117, and CD123 are also expressed on hematopoietic stem cells, CAR T-cell therapy can lead to severe and potentially lethal myeloablation. Novel strategies to combat these issues include creation of bispecific CARs, CAR T-cell “safety switches”, TCR-like CARs, NK CARs, and universal CARs, but all vary in their ability to provide a sustained remission, and consolidation with an allogeneic hematopoietic cell transplantation (allo-HCT) will be necessary in most cases This review highlights the delicate balance between effectively eliminating AML blasts and leukemic stem cells, while preserving the ability for bone marrow to regenerate. The impact of CAR therapy on treatment landscape of AML and changing scope of allo-HCT is discussed. Continued advances in AML CAR therapy would be of great benefit to a disease that still has high morbidity and mortality.
Therapeutic Strategies Targeting Urokinase and Its Receptor in Cancer
Authors: Masucci Maria Teresa , Minopoli Michele , Di Carluccio Gioconda , Motti Maria Letizia , Carriero Maria Vincenza
Published: January 2022

Several studies have ascertained that uPA and uPAR do participate in tumor progression and metastasis and are involved in cell adhesion, migration, invasion and survival, as well as angiogenesis. Increased levels of uPA and uPAR in tumor tissues, stroma and biological fluids correlate with adverse clinic–pathologic features and poor patient outcomes. After binding to uPAR, uPA activates plasminogen to plasmin, a broad-spectrum matrix- and fibrin-degrading enzyme able to facilitate tumor cell invasion and dissemination to distant sites. Moreover, uPAR activated by uPA regulates most cancer cell activities by interacting with a broad range of cell membrane receptors. These findings make uPA and uPAR not only promising diagnostic and prognostic markers but also attractive targets for developing anticancer therapies. In this review, we debate the uPA/uPAR structure–function relationship as well as give an update on the molecules that interfere with or inhibit uPA/uPAR functions. Additionally, the possible clinical development of these compounds is discussed.
Sphingosine Kinase-1 Is Overexpressed and Correlates with Hypoxia in Osteosarcoma: Relationship with Clinicopathological Parameters
Authors: Gomez-Brouchet Anne , Illac Claire , Ledoux Adeline , Fortin Pierre-Yves , de Barros Sandra , Vabre Clémentine , Despas Fabien , Peries Sophie , Casaroli Christelle , Bouvier Corinne , Aubert Sébastien , de Pinieux Gonzague , Larousserie Frédérique , Galmiche Louise , Talmont Franck , Pitson Stuart , Maddelein Marie-Lise , Cuvillier Olivier
Published: January 2022

The Sphingosine kinase-1/Sphingosine 1-Phosphate (SphK1/S1P) signaling pathway is overexpressed in various cancers, and is instrumental for the adaptation to hypoxia in a number of solid tumor models, but no data are available in osteosarcoma. Here we report that SphK1 and the S1P1 receptor are involved in HIF-1α accumulation in hypoxic osteosarcoma cells. FTY720 (Fingolimod), which targets SphK1 and S1P1, prevented HIF-1α accumulation, and also inhibited cell proliferation in both normoxia and hypoxia unlike conventional chemotherapy. In human biopsies, a significant increase of SphK1 activity was observed in cancer compared with normal bones. In all sets of TMA samples (130 cases of osteosarcoma), immunohistochemical analysis showed the hypoxic marker GLUT-1, SphK1 and S1P1 were expressed in tumors. SphK1 correlated with the GLUT-1 suggesting that SphK1 is overexpressed and correlates with intratumoral hypoxia. No correlation was found between GLUT-1 or SphK1 and response to chemotherapy, but a statistical difference was found with increased S1P1 expression in patients with poor response in long bone osteosarcomas. Importantly, multivariate analyses showed that GLUT-1 was associated with an increased risk of death in flat bone, whereas SphK1 and S1P1 were associated with an increased risk of death in long bones.
Closed-System Solution of the 1D Atom from Collision Model
Authors: Maffei Maria , Camati Patrice A. , Auffèves Alexia
Published: January 2022

Obtaining the total wavefunction evolution of interacting quantum systems provides access to important properties, such as entanglement, shedding light on fundamental aspects, e.g., quantum energetics and thermodynamics, and guiding towards possible application in the fields of quantum computation and communication. We consider a two-level atom (qubit) coupled to the continuum of travelling modes of a field confined in a one-dimensional chiral waveguide. Originally, we treated the light-matter ensemble as a closed, isolated system. We solve its dynamics using a collision model where individual temporal modes of the field locally interact with the qubit in a sequential fashion. This approach allows us to obtain the total wavefunction of the qubit-field system, at any time, when the field starts in a coherent or a single-photon state. Our method is general and can be applied to other initial field states.
Parametric Study on Ducted Micro Wind Energy Harvester
Authors: Kim In-Ho , Kim Byeong-Ryong , Yang Yeon-Jae , Jang Seon-Jun
Published: January 2022

Micro wind energy harvester (MWEH) can provide eco-friendly and sustainable energy for low-power electric devices like wireless sensors. The performance of the wind turbine can be enhanced by applying a duct with a brim. In this work, the characteristic study was performed when the duct is applied to the MWEH through the computational fluid dynamics analysis of the internal and external flow of the duct. The power generation performance for various cases was evaluated using the wind tunnel test. It is confirmed that the ducted MWEH is robust to the change of the wind direction and its performance can be further enhanced by the brim.
Managing Moose from Home: Determining Landscape Carrying Capacity for Alces alces Using Remote Sensing
Authors: Kramer David W. , Prebyl Thomas J. , Nibbelink Nathan P. , Miller Karl V. , Royo Alejandro A. , Frair Jacqueline L.
Published: January 2022

In temperate forests of the northeastern U.S., moose (Alces alces) populations are adapted for mixed-age heterogeneous landscapes that provide abundant herbaceous forage in warm months and coniferous forage during winter. Heterogeneity of forest stands is driven by management activities or natural disturbance, resulting in a multi-age forest at a landscape scale. Here, we present a method to estimate landscape carrying capacity of moose by combining remote sensing classification of forest cover class with literature or field-based estimates of class-specific forage abundance. We used Landsat imagery from 1991 to 2013 for the Allegheny National Forest and 2013–2018 for the Adirondack Park, and associated training polygons, to predict based on NDVI and SWI whether a forested landscape fit into one of three cover classes: mature forest, intermediate timber removal, or overstory timber removal. Our three-classes yielded a mean land cover prediction accuracy of 94.3% (Khat = 0.91) and 86.9% (Khat = 0.76) for ANFR and AP, respectively. In the AP, we applied previously calculated summer crude protein values to our predicted cover types, resulting in an estimated average carrying capacity of 760 moose (SD ± 428) across all sampling years, similar in magnitude to a density estimate of 716 moose (95% CI = 566–906) calculated during the same time. Our approach was able to accurately identify forest timber treatments across landscapes at differing spatial and temporal scales and provide an alternative method to estimate landscape-level ungulate carrying capacity. The ability to accurately identify areas of potential conflict from overbrowsing, or to highlight areas in need of land cover treatments can increase the toolset for ungulate management in managed forest landscapes.
Probiotics Supplementation Improves Intestinal Permeability, Obesity Index and Metabolic Biomarkers in Elderly Thai Subjects: A Randomized Controlled Trial
Authors: Chaiyasut Chaiyavat , Sivamaruthi Bhagavathi Sundaram , Lailerd Narissara , Sirilun Sasithorn , Khongtan Suchanat , Fukngoen Pranom , Peerajan Sartjin , Saelee Manee , Chaiyasut Khontaros , Kesika Periyanaina , Sittiprapaporn Phakkharawat
Published: January 2022

Intestinal integrity prevents the diffusion of allergens, toxins, and pathogens from the gastrointestinal lumen into the tissue and the circulatory system. Damage in intestinal integrity may cause mild to serious health issues, such as inflammation, gastrointestinal disorders, neurological diseases, and neurodegenerative disorders. Thus, maintaining a healthy intestinal barrier function is essential to sustain health. Probiotics are known for their ability to protect and restore intestinal permeability in vitro and in vivo. The multi-strain probiotics are more efficient than that of a single strain in terms of their protective efficacy. Therefore, the present study was planned and implemented to study the supplementation of probiotic mix (Lactobacillus paracasei HII01, Bifidobacteriumbreve, and Bifidobacterium longum) on intestinal permeability, lipid profile, obesity index and metabolic biomarkers in elderly Thai subjects. The results revealed that the supplementation of studied probiotics improved the intestinal barrier function (up to 48%), significantly increasing the high-density lipoprotein (HDL)-cholesterol. Moreover, the intervention improved obesity-related anthropometric biomarkers and short-chain fatty acid levels in human subjects. The current study strongly recommends further extended research to confirm the beneficial effect of probiotics, which may pave the way to formulate probiotic-based health supplements to adjuvant the treatment of several metabolic diseases.
Studying Nearby Galactic Planetary Nebulae with MUSE: On the Physical Properties of IC 418
Authors: Monreal-Ibero  Ana , Walsh Jeremy R.
Published: January 2022

Spectroscopic mapping of planetary nebulae (PNe) is particularly useful to capture the richness in terms of physical and chemical properties that exist in these objects. The advent of the multi-unit spectroscopic explorer (MUSE), a large integral field unit mounted on the ESO Very Large Telescope, allow us to obtain this information over the whole face of galactic PNe in a reasonable amount of time. This in turn reveals a wealth of information that can bring insight into this structural complexity. Here we discuss new results from commissioning data for the physical properties of IC 418 and succinctly review recently published results on two additional targets (NGC 3132 and NGC 7009). For the newly-analysed PN, electron densities are high with ne([S ii]) displaying a completely different structure than ne([Cl iii]). The electron temperature was relatively uniform, but somewhat higher at the rim as measured by two of the three used diagnostics ([S iii] 6312/9069, and [Ar iii] 5192/7136). The joint results for the three PNe amply illustrate the potential of MUSE for the study of galactic PNe.
The Organization of the Pig T-Cell Receptor γ (TRG) Locus Provides Insights into the Evolutionary Patterns of the TRG Genes across Cetartiodactyla
Authors: Linguiti Giovanna , Giannico Francesco , D’Addabbo Pietro , Pala Angela , Caputi Jambrenghi Anna , Ciccarese Salvatrice , Massari Serafina , Antonacci Rachele
Published: January 2022

The domestic pig (Sus scrofa) is a species representative of the Suina, one of the four suborders within Cetartiodactyla. In this paper, we reported our analysis of the pig TRG locus in comparison with the loci of species representative of the Ruminantia, Tylopoda, and Cetacea suborders. The pig TRG genomic structure reiterates the peculiarity of the organization of Cetartiodactyla loci in TRGC “cassettes”, each containing the basic V-J-J-C unit. Eighteen genes arranged in four TRGC cassettes, form the pig TRG locus. All the functional TRG genes were expressed, and the TRGV genes preferentially rearrange with the TRGJ genes within their own cassette, which correlates the diversity of the γ-chain repertoire with the number of cassettes. Among them, the TRGC5, located at the 5′ end of the locus, is the only cassette that retains a marked homology with the corresponding TRGC cassettes of all the analyzed species. The preservation of the TRGC5 cassette for such a long evolutionary time presumes a highly specialized function of its genes, which could be essential for the survival of species. Therefore, the maintenance of this cassette in pigs confirms that it is the most evolutionarily ancient within Cetartiodactyla, and it has undergone a process of duplication to give rise to the other TRGC cassettes in the different artiodactyl species in a lineage-specific manner.
Airway Delivery of Hydrogel-Encapsulated Niclosamide for the Treatment of Inflammatory Airway Disease
Authors: Ousingsawat Jiraporn , Centeio Raquel , Cabrita Inês , Talbi Khaoula , Zimmer Oliver , Graf Moritz , Göpferich Achim , Schreiber Rainer , Kunzelmann Karl
Published: January 2022

Repurposing of the anthelminthic drug niclosamide was proposed as an effective treatment for inflammatory airway diseases such as asthma, cystic fibrosis, and chronic obstructive pulmonary disease. Niclosamide may also be effective for the treatment of viral respiratory infections, such as SARS-CoV-2, respiratory syncytial virus, and influenza. While systemic application of niclosamide may lead to unwanted side effects, local administration via aerosol may circumvent these problems, particularly when the drug is encapsulated into small polyethylene glycol (PEG) hydrospheres. In the present study, we examined whether PEG-encapsulated niclosamide inhibits the production of mucus and affects the pro-inflammatory mediator CLCA1 in mouse airways in vivo, while effects on mucociliary clearance were assessed in excised mouse tracheas. The potential of encapsulated niclosamide to inhibit TMEM16A whole-cell Cl− currents and intracellular Ca2+ signalling was assessed in airway epithelial cells in vitro. We achieved encapsulation of niclosamide in PEG-microspheres and PEG-nanospheres (Niclo-spheres). When applied to asthmatic mice via intratracheal instillation, Niclo-spheres strongly attenuated overproduction of mucus, inhibited secretion of the major proinflammatory mediator CLCA1, and improved mucociliary clearance in tracheas ex vivo. These effects were comparable for niclosamide encapsulated in PEG-nanospheres and PEG-microspheres. Niclo-spheres inhibited the Ca2+ activated Cl− channel TMEM16A and attenuated mucus production in CFBE and Calu-3 human airway epithelial cells. Both inhibitory effects were explained by a pronounced inhibition of intracellular Ca2+ signals. The data indicate that poorly dissolvable compounds such as niclosamide can be encapsulated in PEG-microspheres/nanospheres and deposited locally on the airway epithelium as encapsulated drugs, which may be advantageous over systemic application.
Fat and Protein Combat Triggers Immunological Weapons of Innate and Adaptive Immune Systems to Launch Neuroinflammation in Parkinson’s Disease
Authors: Hatton Shelby Loraine , Pandey Manoj Kumar
Published: January 2022

Parkinson’s disease (PD) is the second-most common neurodegenerative disease in the world, affecting up to 10 million people. This disease mainly happens due to the loss of dopaminergic neurons accountable for memory and motor function. Partial glucocerebrosidase enzyme deficiency and the resultant excess accumulation of glycosphingolipids and alpha-synuclein (α-syn) aggregation have been linked to predominant risk factors that lead to neurodegeneration and memory and motor defects in PD, with known and unknown causes. An increasing body of evidence uncovers the role of several other lipids and their association with α-syn aggregation, which activates the innate and adaptive immune system and sparks brain inflammation in PD. Here, we review the emerging role of a number of lipids, i.e., triglyceride (TG), diglycerides (DG), glycerophosphoethanolamines (GPE), polyunsaturated fatty acids (PUFA), sphingolipids, gangliosides, glycerophospholipids (GPL), and cholesterols, and their connection with α-syn aggregation as well as the induction of innate and adaptive immune reactions that trigger neuroinflammation in PD.
Regional Impact of COVID-19-Associated Pulmonary Aspergillosis (CAPA) during the First Wave
Authors: Bentvelsen Robbert G. , Arkel Andreas L. E. Van , Rijpstra Tom A. , Kant Merijn K. M. , Brugge Simone Van Der Sar-Van Der , Loth Daan W. , Van Wijngaarden Peter , Mée Arthur W. F. Du , Yick David C. Y. , Diederen Bram M. W. , Wever Peter C. , Leenders Alexander C. A. P. , Van Dommelen Laura , Groot Klaas H. De , Van den Bijllaardt Wouter , Verweij Paul E.
Published: January 2022

Background: Critically ill COVID-19 patients have proven to be at risk for developing invasive fungal infections. However, the incidence and impact of possible/probable COVID-19-associated pulmonary aspergillosis (CAPA) in severe COVID-19 patients varies between cohorts. We aimed to assess the incidence, risk factors, and clinical outcome of invasive pulmonary aspergillosis in a regional cohort of COVID-19 intensive care patients. Methods: We performed a regional, multicentre, retrospective cohort study in the intensive care units (ICUs) in North Brabant, The Netherlands. We included adult patients with rt-PCR-confirmed SARS-CoV-2 infection (COVID-19), requiring mechanical ventilation for acute respiratory distress syndrome. Demographics, clinical course, biomarker value, and treatment outcomes were compared between the groups with possible/probable CAPA from the main study centre and the regional centres, and without signs of CAPA from the main study centre as controls. The primary aim was to assess the regional impact of possible/probable CAPA in COVID-19 ICU patients, measured as all-cause mortality at 30 days after ICU admission. Secondary outcomes were risk factors for developing CAPA, based on underlying host factors and to identify the value of the mycological arguments for the diagnosing of CAPA. Results: Between 1 March and 30 April 2020, we included 123 patients with severe COVID-19: 29 patients (30.9%) in the main ICU with possible/probable CAPA, and 65 (69.1%) with no signs of CAPA; 29 patients in the regional ICUs with signs of CAPA. Patients’ characteristics and risk factors did not differ for CAPA and non-CAPA patients. Patients with COPD and/or chronic steroid medication developed CAPA more frequently, although this was not statistically significant. CAPA patients were admitted to the ICU earlier, had lower PF-ratios, and more often required renal replacement therapy. All-cause 30-day mortality was significantly higher in mechanically ventilated COVID-19 patients with possible/probable CAPA 39.7% (23/58) compared to patients without evidence for CAPA 16.9% (11/65) (OR 3.2 [95% CI 1.4–7.4] p = 0.005). Conclusion: The high incidence of possible and probable CAPA in critically ill COVID-19 patients is alarming. The increase in 30-day mortality in CAPA highlights the need for active surveillance and management strategies in critically ill COVID-19 patients.