bluuebunny/crossref_metadata_2025 huggingface dataset:
Transduction and Genome Editing of the Heart with Adeno-Associated Viral Vectors Loaded onto Electrospun Polydioxanone Nonwoven Fabrics
Authors: Furuno Kotoko , Suzuki Keiichiro , Sakai Shinji
Published: April 2024

In this study, we introduce electrospun polydioxanone (PDO) nonwoven fabrics as a platform for the delivery of adeno-associated virus (AAV) vectors for transduction and genome editing by adhering them to organ surfaces, including the heart. AAV vectors were loaded onto the PDO fabrics by soaking the fabrics in a solution containing AAV vectors. In vitro, the amount of AAV vectors loaded onto the fabrics could be adjusted by changing their concentration in the solution, and the number of cells expressing the green fluorescent protein (GFP) encoded by the AAV vectors increased in correlation with the increasing amount of loaded AAV vectors. In vivo, both transduction and genome editing resulted in the observation of GFP expression around AAV vector-loaded PDO fabrics attached to the surfaces of mouse hearts, indicating effective transduction and expression at the target site. These results demonstrate the great potential of electrospun PDO nonwoven fabrics carrying therapeutic AAV vectors for gene therapy.
Phenotypic Variability of Andersen–Tawil Syndrome Due to Allelic Mutation c.652C>T in the KCNJ2 Gene—A New Family Case Report
Authors: Onore Maria Elena , Picillo Esther , D’Ambrosio Paola , Morra Salvatore , Nigro Vincenzo , Politano Luisa
Published: April 2024

Andersen–Tawil syndrome (ATS) is a multisystem channelopathy characterized by periodic paralysis, ventricular arrhythmias, prolonged QT interval, and facial dysmorphisms occurring in the first/second decade of life. High phenotypic variability and incomplete penetrance of the genes causing the disease make its diagnosis still a challenge. We describe a three-generation family with six living individuals affected by ATS. The proband is a 37-year-old woman presenting since age 16, with episodes of muscle weakness and cramps in the pre-menstrual period. The father, two brothers, one paternal uncle and one cousin also complained of cramps, muscle stiffness, and weakness. Despite normal serum potassium concentration, treatment with potassium, magnesium, and acetazolamide alleviated paralysis attacks suggesting a dyskalemic syndrome. Dysmorphic features were noted in the proband, only later. On the ECG, all but one had normal QT intervals. The affected males developed metabolic syndrome or obesity. The father had two myocardial infarctions and was implanted with an intracardiac cardioverter defibrillator (ICD). A genetic investigation by WES analysis detected the heterozygous pathogenic variant (NM_000891.2: c.652C>T, p. Arg218Trp) in the KCNJ2 gene related to ATS, confirmed by segregation studies in all affected members. Furthermore, we performed a review of cases with the same mutation in the literature, looking for similarities and divergences with our family case.
The Role of Cdo1 in Ferroptosis and Apoptosis in Cancer
Authors: Chen Xiaoyi , Poetsch Ansgar
Published: April 2024

Cysteine dioxygenase type 1 (Cdo1) is a tumor suppressor gene. It regulates the metabolism of cysteine, thereby influencing the cellular antioxidative capacity. This function puts Cdo1 in a prominent position to promote ferroptosis and apoptosis. Cdo1 promotes ferroptosis mainly by decreasing the amounts of antioxidants, leading to autoperoxidation of the cell membrane through Fenton reaction. Cdo1 promotes apoptosis mainly through the product of cysteine metabolism, taurine, and low level of antioxidants. Many cancers exhibit altered function of Cdo1, underscoring its crucial role in cancer cell survival. Genetic and epigenetic alterations have been found, with methylation of Cdo1 promoter as the most common mutation. The fact that no cancer was found to be caused by altered Cdo1 function alone indicates that the tumor suppressor role of Cdo1 is mild. By compiling the current knowledge about apoptosis, ferroptosis, and the role of Cdo1, this review suggests possibilities for how the mild anticancer role of Cdo1 could be harnessed in new cancer therapies. Here, developing drugs targeting Cdo1 is considered meaningful in neoadjuvant therapies, for example, helping against the development of anti-cancer drug resistance in tumor cells.
The Roles of AGTRAP, ALKBH3, DIVERSIN, NEDD8 and RRM1 in Glioblastoma Pathophysiology and Prognosis
Authors: Dumitru Claudia Alexandra , Walter Nikolas , Siebert Carl Ludwig Raven , Schäfer Frederik Till Alexander , Rashidi Ali , Neyazi Belal , Stein Klaus-Peter , Mawrin Christian , Sandalcioglu Ibrahim Erol
Published: April 2024

This study determined the expression of five novel biomarker candidates in IDH wild-type glioblastoma (GBM) tissues compared to non-malign brain parenchyma, as well as their prognostic relevance for the GBM patients’ outcomes. The markers were analysed by immunohistochemistry in tumour tissues (n = 186) and healthy brain tissues (n = 54). The association with the patients’ overall survival (OS) and progression-free survival (PFS) was assessed by Kaplan–Meier and log-rank test. The prognostic value of the markers was determined using multivariate Cox proportional hazard models. AGTRAP, DIVERSIN, cytoplasmic NEDD8 (NEDD8c) and RRM1 were significantly overexpressed in tumour tissues compared to the healthy brain, while the opposite was observed for ALKBH3. AGTRAP, ALKBH3, NEDD8c and RRM1 were significantly associated with OS in univariate analysis. AGTRAP and RRM1 were also independent prognostic factors for OS in multivariate analysis. For PFS, only AGTRAP and NEDD8c reached significance in univariate analysis. Additionally, AGTRAP was an independent prognostic factor for PFS in multivariate models. Finally, combined analysis of the markers enhanced their prognostic accuracy. The combination AGTRAP/ALKBH3 had the strongest prognostic value for the OS of GBM patients. These findings contribute to a better understanding of the GBM pathophysiology and may help identify novel therapeutic targets in this type of cancer.
Review of Vision-Based Environmental Perception for Lower-Limb Exoskeleton Robots
Authors: Wang Chen , Pei Zhongcai , Fan Yanan , Qiu Shuang , Tang Zhiyong
Published: April 2024

The exoskeleton robot is a wearable electromechanical device inspired by animal exoskeletons. It combines technologies such as sensing, control, information, and mobile computing, enhancing human physical abilities and assisting in rehabilitation training. In recent years, with the development of visual sensors and deep learning, the environmental perception of exoskeletons has drawn widespread attention in the industry. Environmental perception can provide exoskeletons with a certain level of autonomous perception and decision-making ability, enhance their stability and safety in complex environments, and improve the human–machine–environment interaction loop. This paper provides a review of environmental perception and its related technologies of lower-limb exoskeleton robots. First, we briefly introduce the visual sensors and control system. Second, we analyze and summarize the key technologies of environmental perception, including related datasets, detection of critical terrains, and environment-oriented adaptive gait planning. Finally, we analyze the current factors limiting the development of exoskeleton environmental perception and propose future directions.
Complex Spatial Illumination Scheme Optimization of Backscattering Mueller Matrix Polarimetry for Tissue Imaging and Biosensing
Authors: Jiao Wei , Zhang Zheng , Zeng Nan , Hao Rui , He Honghui , He Chao , Ma Hui
Published: April 2024

Polarization imaging and sensing techniques have shown great potential for biomedical and clinical applications. As a novel optical biosensing technology, Mueller matrix polarimetry can provide abundant microstructural information of tissue samples. However, polarimetric aberrations, which lead to inaccurate characterization of polarization properties, can be induced by uneven biomedical sample surfaces while measuring Mueller matrices with complex spatial illuminations. In this study, we analyze the detailed features of complex spatial illumination-induced aberrations by measuring the backscattering Mueller matrices of experimental phantom and tissue samples. We obtain the aberrations under different spatial illumination schemes in Mueller matrix imaging. Furthermore, we give the corresponding suggestions for selecting appropriate illumination schemes to extract specific polarization properties, and then provide strategies to alleviate polarimetric aberrations by adjusting the incident and detection angles in Mueller matrix imaging. The optimized scheme gives critical criteria for the spatial illumination scheme selection of non-collinear backscattering Mueller matrix measurements, which can be helpful for the further development of quantitative tissue polarimetric imaging and biosensing.
The Influence of Muslim and Christian Destinations on Tourists’ Behavioural Intentions and Risk Perceptions
Authors: Carballo Rita R. , León Carmelo J. , Carballo María M.
Published: April 2024

This paper studies the factors that influence tourists’ risk perceptions of various destinations with different attributes and sociocultural profiles. Factor analysis is utilised to investigate the determinants of risk perceptions, finding that they are influenced both by the type of risk (delinquency, health, accident, environment and catastrophe) and by the characteristics of the destination regarding the management of risk. Structural equations modelling is conducted to study the relationships between risk perceptions, destination image and visiting intentions across destinations. Multi-group analysis across different destinations proves that tourists’ risk perceptions have different influences on destination image and visiting intentions. The results show that there are significant differences according to the predominant religion at the destinations, i.e., Muslin and Christian. The implication is that different dimensions of perceived risks and destination socio-culture contexts have different influences on the behaviour of tourists.
Does the Cognitive Reflection Test Work with Chinese College Students? Evidence from a Time-Limited Study
Authors: Li Zhaoxian , Yan Shangsong , Liu Jie , Bao Wei , Luo Junlong
Published: April 2024

The cognitive reflection test (CRT) is an experiment task commonly used in Western countries to test intuitive and analytical thinking styles. However, the validity of this task for Chinese participants has not been explored. Therefore, this study recruited Chinese college students to finish CRT tasks with various experimental designs. To gauge the accuracy of the CRT tasks, 438 Chinese college students first completed online questionnaires. Participants were then invited to participate in an offline laboratory with the same experimental settings. Finally, time pressure was used to strictly control intuition and analytical thinking to explore the performance of Chinese college students on CRT tasks. The results show that of the three experiments, Chinese college students had the highest accuracy in the offline test, and the CRT’s intuitive conflict problem still applies to Chinese students under the time-limited condition. This study demonstrates the validity of the CRT in China and proves that time pressure is an effective method for identifying individuals with strong logic ability.
Rest to Promote Learning: A Brain Default Mode Network Perspective
Authors: Luo Wei , Liu Biao , Tang Ying , Huang Jingwen , Wu Ji
Published: April 2024

The brain often switches freely between focused attention and divergent thinking, and the Default Mode Network (DMN) is activated during brain rest. Since its discovery, the DMN, together with its function and characteristics, indicates that learning does not stop when the brain “rests”. Therefore, DMN plays an important role in learning. Neural activities such as beta wave rhythm regulation, “subconscious” divergence thinking mode initiation, hippocampal function, and neural replay occur during default mode, all of which explains that “rest” promotes learning. This paper summarized the function and neural mechanism of DMN in learning and proposed that the DMN plays an essential role in learning, which is that it enables rest to promote learning.
Unveiling Gender-Based Musculoskeletal Disorders in the Construction Industry: A Comprehensive Analysis
Authors: Paramasivam Suresh Kumar , Mani Kanitha , Paneerselvam Balamurugan
Published: April 2024

Without physically intensive building, modern infrastructure development would be impossible. Musculoskeletal diseases (MSDs) and other occupational health issues may arise in such a demanding environment. Construction workers often develop MSDs from repeated actions, uncomfortable postures, and heavy lifting. Musculoskeletal disorders may damage muscles, bones, tendons, ligaments, etc. The effect of MSDs is well known; occupational health studies increasingly include gender-specific aspects. Despite being in the minority, the number of female construction employees is growing. However, physiological variations and occupational activities and environments may provide distinct obstacles. Thus, identifying gender-specific MSDs in construction is essential for worker safety. This research proposes a gender-specific machine learning (ML)-based musculoskeletal disorder detection framework (GS-ML-MSD2F) in the construction industry. A simple random selection procedure chose 250 female and 250 male rebar workers with at least six months of experience for the dataset. In January and June 2023, face-to-face interviews and ergonomic evaluations were undertaken. The data were analyzed using different machine learning methods, and the effectiveness of the methods was studied. The data showed that 60% of female rebar workers had MSD symptoms. The lower back and shoulders accounted for 40% of cases. Multiple machine learning methods revealed two significant factors related to musculoskeletal disorders: lengthy working hours and uncomfortable postures, and long working hours had an adjusted odds ratio of 8.5%, whereas awkward posture had an adjusted odds ratio of 42.5%. These results emphasize the relevance of working hours and posture in MSD prevention for female rebar workers in the construction sector.
Seismic Isolation of Fragile Pole-Type Structures by Rocking with Base Restraints
Authors: Li Sheng , Hu Yao , Lu Zhicheng , Song Bo , Huang Guozhong
Published: April 2024

Pole-type structures are vulnerable to earthquake events due to their slender shapes, particularly porcelain cylindrical equipment in electrical substations, which has inherent fragility and low strength in its materials. Traditional base isolation designs configure the bottom of the pole-type equipment as hinges with restraints. It fully relies on the restrainers to re-center the pole-type equipment, posing a risk of tilting and functionality failure after earthquakes. This study proposes a solution to this challenge by introducing a restrained rocking mechanism at the base of the structure. The design leverages the self-centering nature of rocking motion and uses restrainers to control the amplitude of rotation. Hence, it can effectively avoid tilting of the pole-type structures after earthquakes. Experimental investigations conducted on a 1:1 full-scale specimen revealed that the proposed restrained rocking design can achieve a reduction in seismic internal forces of over 50% while maintaining equipment in an upright position. Furthermore, an analytical model for the proposed isolation system of pole structures was developed and validated through comparison with experimental results. This paper introduces a novel solution for seismic isolation of pole-type structures through restrained rocking, specifically addressing the research gap regarding a reliable self-centering mechanism under seismic excitation. This advancement significantly enhances the seismic resilience of fragile pole-type structures and provides practical design methodologies for the seismic isolation of slender structures.
Numerical Study on Permeability of Reconstructed Porous Concrete Based on Lattice Boltzmann Method
Authors: Zhao Danni , Xu Jiangbo , Wang Xingang , Guo Qingjun , Li Yangcheng , Han Zemin , Liu Yifan , Zhang Zixuan , Zhang Jiajun , Sun Runtao
Published: April 2024

The reconstruction of the porous media model is crucial for researching the mesoscopic seepage characteristics of porous concrete. Based on a self-compiled MATLAB program, a porous concrete model was modeled by controlling four parameters (distribution probability, growth probability, probability density, and porosity) with clear physical meanings using a quartet structure generation set (QSGS) along with the lattice Boltzmann method (LBM) to investigate permeability. The rationality of the numerical model was verified through Poiseuille flow theory. The results showed that the QSGS model exhibited varied pore shapes and disordered distributions, resembling real porous concrete. Seepage velocity distribution showed higher values in larger pores, with flow rates reaching up to 0.012 lattice point velocity. The permeability–porosity relationship demonstrated high linearity (the Pearson correlation coefficient is 0.92), consistent with real porous concrete behavior. The integration of QSGS-LBM represents a novel approach, and the research results can provide new ideas and new means for subsequent research on the permeability of porous concrete or similar porous medium materials.
Effectiveness of Vibration-Based Techniques for Damage Localization and Lifetime Prediction in Structural Health Monitoring of Bridges: A Comprehensive Review
Authors: Rabi Raihan Rahmat , Vailati Marco , Monti Giorgio
Published: April 2024

Bridges are essential to infrastructure and transportation networks, but face challenges from heavier traffic, higher speeds, and modifications like busway integration, leading to potential overloading and costly maintenance. Structural Health Monitoring (SHM) plays a crucial role in assessing bridge conditions and predicting failures to maintain structural integrity. Vibration-based condition monitoring employs non-destructive, in situ sensing and analysis of system dynamics across time, frequency, or modal domains. This method detects changes indicative of damage or deterioration, offering a proactive approach to maintenance in civil engineering. Such monitoring systems hold promise for optimizing the management and upkeep of modern infrastructure, potentially reducing operational costs. This paper aims to assist newcomers, practitioners, and researchers in navigating various methodologies for damage identification using sensor data from real structures. It offers a comprehensive review of prevalent anomaly detection approaches, spanning from traditional techniques to cutting-edge methods. Additionally, it addresses challenges inherent in Vibration-Based Damage (VBD) SHM applications, including establishing damage thresholds, corrosion detection, and sensor drift.
P16/Ki67 Dual Staining in Glandular Cell Abnormalities of the Uterine Cervix
Authors: Jeromel Saša , Repše Fokter Alenka , Dovnik Andraž
Published: April 2024

Very limited information exists about the role of p16/Ki67 dual staining on glandular cells in detecting glandular precancerous lesions and cervical adenocarcinoma. In this study, we investigated the diagnostic accuracy of p16/Ki67 dual staining for the detection of glandular and squamous lesions on the uterine cervix and for cancer of the upper reproductive tract. We performed a retrospective analysis of prospectively collected data on 96 patients with glandular cell abnormalities. We analyzed the diagnostic accuracy of p16/Ki67 dual staining for atypical glandular cells, not otherwise specified (AGC-NOS); atypical glandular cells, favor neoplastic (AGC-FN); adenocarcinoma in situ (AIS); and A-CA (cervical adenocarcinoma). A separate analysis for the detection of squamous precancerous lesions and squamous-cell carcinoma (CIN3+) and for cancer of the upper reproductive tract (EC/OC) was performed. Among patients who had normal histology or a low-grade lesion on final analysis, only 8.5% had positive dual staining. On the other hand, 85.7% of patients with AIS+ on final histology had positive dual staining. The respective specificities of p16/Ki67 dual staining on AGC-NOS for the detection of AIS+ (adenocarcinoma in situ or cervical adenocarcinoma), CIN3+ and EC/OC were 91.5%, 88.7% and 86.4%. High specificity values of p16/Ki67 dual staining on cervical smears labelled as AGC-NOS for the detection of CIN3+ and AIS+ suggest that this method might be a useful addition in cervical cancer screening.
Taurine-Functionalized Carbon Nanotubes as Electrode Catalysts for Improvement in the Performance of Vanadium Redox Flow Battery
Authors: Wei Lian , Liu Tao , Zhang Yimin , Liu Hong , Ge Ling
Published: April 2024

The vanadium redox flow battery (VRFB) is a highly favorable tool for storing renewable energy, and the catalytic activity of electrode materials is crucial for its development. Taurine-functionalized carbon nanotubes (CNTs) were prepared with the aim of augmenting the redox process of vanadium ions and enhancing the efficiency of the VRFB. Sulfonated CNTs were synthesized through a simple modification process in a taurine solution and used as electrocatalysts for redox reactions involving VO2+/VO2+ and V2+/V3+. The SO3H-CNTs modified at 60 °C for 2 h exhibit the best electrocatalytic activity, showing higher redox peak current values compared to pristine carboxylated CNTs (COOH-CNTs). Sulfonic acid groups added to the surface of CNTs increase active sites for redox reactions and act as carriers for mass transfer and bridges for charge transfer, accelerating the rate of the electrode reactions. A battery consisting of SO3H-CNTs as catalysts demonstrates the outstanding charge–discharge performance at a current density of 300 mA∙cm−2. This configuration displays voltage and energy efficiencies of 81.46% and 78.83%, respectively, representing enhancements of 6.15% and 6.12% compared to that equipped with conventional graphite felts (75.31%, 72.71%). This study illustrates that taurine-functionalized carbon nanotubes serve as an efficient and promising catalyst for both the anode and cathode, leading to the improved performance of the VRFB.
Catalytic Decomposition of CH4 to Hydrogen and Carbon Nanotubes Using the Pt(1)-Fe(30)/MCM-41 Catalyst
Authors: Seo Ho Joon
Published: April 2024

The catalytic decomposition of CH4 to H2 and carbon nanotubes (CNTs) was investigated regarding Pt(1)-Fe(30)/MCM-41 and Fe(30)/MCM-41 using a fixed-bed flow reactor under an atmosphere. X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), transmission electron microscope (TEM), and Raman spectroscopy were used to characterize the behavior of Pt(1)-Fe(30)/MCM-41 and Fe(30)/MCM-41. The hydrogen yield of Pt(1)-Fe(30)/MCM-41 was 3.2 times higher than that of Fe(30)/MCM-41. When 1 wt% of Pt was added to Fe(30)/MCM-41(Mobil Composition of Matter No. 41), the atomic percentage of Fe2p increased from 13.39% to 16.14% and the core Fe2p1/2 electron levels of Fe0 and Fe2+ chemically shifted to lower energies (0.2 eV and 0.1 eV, respectively) than those of Fe(30)/MCM-41. The Fe, Pt, Si, and O nanoparticles were uniformly distributed on the catalyst surface, and the average iron particle sizes of the Pt(1)-Fe(30)/MCM-41 and Fe(30)/MCM-41 were about 33.4 nm and 58.5 nm, respectively. This is attributed to the uniform distribution of the nano-sized iron particles on the MCM-41 surface, which was due to the suitable metal-carrier interaction (SMCI) between Fe, Pt, and MCM-41 and the high reduction degree of Fe due to the spillover effect of H2 from Pt to Fe. Pt(1)-Fe(30)/MCM-41 produced multiwalled CNTs and bamboo-shaped CNTs with high crystallinity and graphitization degree using the tip-growth mechanism, with an ID/IG ratio of 0.93 and a C(101)/C(002) ratio of 0.64.
The Expression and Secretion Profile of TRAP5 Isoforms in Gaucher Disease
Authors: Ivanova Margarita M. , Dao Julia , Loynab Neala , Noor Sohailla , Kasaci Neil , Friedman Andrew , Goker-Alpan Ozlem
Published: April 2024

Background: Gaucher disease (GD) is caused by glucocerebrosidase (GCase) enzyme deficiency, leading to glycosylceramide (Gb-1) and glucosylsphingosine (Lyso-Gb-1) accumulation. The pathological hallmark for GD is an accumulation of large macrophages called Gaucher cells (GCs) in the liver, spleen, and bone marrow, which are associated with chronic organ enlargement, bone manifestations, and inflammation. Tartrate-resistant acid phosphatase type 5 (TRAP5 protein, ACP5 gene) has long been a nonspecific biomarker of macrophage/GCs activation; however, the discovery of two isoforms of TRAP5 has expanded its significance. The discovery of TRAP5′s two isoforms revealed that it is more than just a biomarker of macrophage activity. While TRAP5a is highly expressed in macrophages, TRAP5b is secreted by osteoclasts. Recently, we have shown that the elevation of TRAP5b in plasma is associated with osteoporosis in GD. However, the role of TRAP isoforms in GD and how the accumulation of Gb-1 and Lyso-Gb-1 affects TRAP expression is unknown. Methods: 39 patients with GD were categorized into cohorts based on bone mineral density (BMD). TRAP5a and TRAP5b plasma levels were quantified by ELISA. ACP5 mRNA was estimated using RT-PCR. Results: An increase in TRAP5b was associated with reduced BMD and correlated with Lyso-Gb-1 and immune activator chemokine ligand 18 (CCL18). In contrast, the elevation of TRAP5a correlated with chitotriosidase activity in GD. Lyso-Gb-1 and plasma seemed to influence the expression of ACP5 in macrophages. Conclusions: As an early indicator of BMD alteration, measurement of circulating TRAP5b is a valuable tool for assessing osteopenia–osteoporosis in GD, while TRAP5a serves as a biomarker of macrophage activation in GD. Understanding the distinct expression pattern of TRAP5 isoforms offers valuable insight into both bone disease and the broader implications for immune system activation in GD.
Expression of PDLIM5 Spliceosomes and Regulatory Functions on Myogenesis in Pigs
Authors: Fu Yu , Li Shixin , Nie Jingru , Yan Dawei , Zhang Bo , Hao Xin , Zhang Hao
Published: April 2024

Meat yield, determined by muscle growth and development, is an important economic trait for the swine industry and a focus of research in animal genetics and breeding. PDZ and LIM domain 5 (PDLIM5) are cytoskeleton-related proteins that play key roles in various tissues and cells. These proteins have multiple isoforms, primarily categorized as short (PDLIM5-short) and long (PDLIM5-long) types, distinguished by the absence and presence of an LIM domain, respectively. However, the expression patterns of swine PDLIM5 isoforms and their regulation during porcine skeletal muscle development remain largely unexplored. We observed that PDLIM5-long was expressed at very low levels in pig muscles and that PDLIM5-short and total PDLIM5 were highly expressed in the muscles of slow-growing pigs, suggesting that PDLIM5-short, the dominant transcript in pigs, is associated with a slow rate of muscle growth. PDLIM5-short suppressed myoblast proliferation and myogenic differentiation in vitro. We also identified two single nucleotide polymorphisms (−258 A > T and −191 T > G) in the 5′ flanking region of PDLIM5, which influenced the activity of the promoter and were associated with muscle growth rate in pigs. In summary, we demonstrated that PDLIM5-short negatively regulates myoblast proliferation and differentiation, providing a theoretical basis for improving pig breeding programs.
Drosophila Contributions towards Understanding Neurofibromatosis 1
Authors: Atsoniou Kalliopi , Giannopoulou Eleni , Georganta Eirini-Maria , Skoulakis Efthimios M. C.
Published: April 2024

Neurofibromatosis 1 (NF1) is a multisymptomatic disorder with highly variable presentations, which include short stature, susceptibility to formation of the characteristic benign tumors known as neurofibromas, intense freckling and skin discoloration, and cognitive deficits, which characterize most children with the condition. Attention deficits and Autism Spectrum manifestations augment the compromised learning presented by most patients, leading to behavioral problems and school failure, while fragmented sleep contributes to chronic fatigue and poor quality of life. Neurofibromin (Nf1) is present ubiquitously during human development and postnatally in most neuronal, oligodendrocyte, and Schwann cells. Evidence largely from animal models including Drosophila suggests that the symptomatic variability may reflect distinct cell-type-specific functions of the protein, which emerge upon its loss, or mutations affecting the different functional domains of the protein. This review summarizes the contributions of Drosophila in modeling multiple NF1 manifestations, addressing hypotheses regarding the cell-type-specific functions of the protein and exploring the molecular pathways affected upon loss of the highly conserved fly homolog dNf1. Collectively, work in this model not only has efficiently and expediently modelled multiple aspects of the condition and increased understanding of its behavioral manifestations, but also has led to pharmaceutical strategies towards their amelioration.
Correction: Dastghaib et al. Simvastatin Induces Unfolded Protein Response and Enhances Temozolomide-Induced Cell Death in Glioblastoma Cells. Cells 2020, 9, 2339
Authors: Dastghaib Sanaz , Shojaei Shahla , Mostafavi-Pour Zohreh , Sharma Pawan , Patterson John B. , Samali Afshin , Mokarram Pooneh , Ghavami Saeid
Published: April 2024

In the original publication [...]
High Level of CD8+PD-1+ Cells in Patients with Chronic Myeloid Leukemia Who Experienced Loss of MMR after Imatinib Discontinuation
Authors: Kwaśnik Paulina , Zaleska Joanna , Link-Lenczowska Dorota , Zawada Magdalena , Wysogląd Hubert , Ochrem Bogdan , Bober Grażyna , Wasilewska Ewa , Hus Iwona , Szarejko Monika , Prejzner Witold , Grzybowska-Izydorczyk Olga , Klonowska-Szymczyk Agnieszka , Mędraś Ewa , Kiełbus Michał , Sacha Tomasz , Giannopoulos Krzysztof
Published: April 2024

Treatment-free remission (TFR) is achieved in approximately half of chronic myeloid leukemia (CML) patients treated with tyrosine kinase inhibitors. The mechanisms responsible for TFR maintenance remain elusive. This study aimed to identify immune markers responsible for the control of residual CML cells early in the TFR (at 3 months), which may be the key to achieving long-term TFR and relapse-free survival (RFS) after discontinuation of imatinib. Our study included 63 CML patients after imatinib discontinuation, in whom comprehensive analysis of changes in the immune system was performed by flow cytometry, and changes in the BCR::ABL1 transcript levels were assessed by RQ-PCR and ddPCR. We demonstrated a significant increase in the percentage of CD8+PD-1+ cells in patients losing TFR. The level of CD8+PD-1+ cells is inversely related to the duration of treatment and incidence of deep molecular response (DMR) before discontinuation. Analysis of the ROC curve showed that the percentage of CD8+PD-1+ cells may be a significant factor in early molecular recurrence. Interestingly, at 3 months of TFR, patients with the e13a2 transcript had a significantly higher proportion of the PD-1-expressing immune cells compared to patients with the e14a2. Our results suggest the important involvement of CD8+PD-1+ cells in the success of TFR and may help in identifying a group of patients who could successfully discontinue imatinib.
DNA Methylation Profiles Are Stable in H3 K27M-Mutant Diffuse Midline Glioma Neurosphere Cell Lines
Authors: Schniederjan Matthew J. , Potnis Cahil , Vasudevaraja Varshini , Moser Catherine D. , Watson Bethany , Snuderl Matija , MacDonald Tobey , Rogers Beverly B.
Published: April 2024

Diffuse midline gliomas are among the deadliest human cancers and have had little progress in treatment in the last 50 years. Cell cultures of these tumors have been developed recently, but the degree to which such cultures retain the characteristics of the source tumors is unknown. DNA methylation profiling offers a powerful tool to look at genome-wide epigenetic changes that are biologically meaningful and can help assess the similarity of cultured tumor cells to their in vivo progenitors. Paraffinized diagnostic tissue from three diffuse intrinsic pontine gliomas with H3 K27M mutations was compared with subsequent passages of neurosphere cell cultures from those tumors. Each cell line was passaged 3–4 times and analyzed with DNA methylation arrays and standard algorithms that provided a comparison of diagnostic classification and cluster analysis. All samples tested maintained high classifier scores and clustered within the reference group of H3 K27M-mutant diffuse midline gliomas. There was a gain of 1q in all cell lines, with two cell lines initially manifesting the gain of 1q only during culture. In vitro cell cultures of H3 K27M-mutant gliomas maintain high degrees of similarity in DNA methylation profiles to their source tumor, confirming their fidelity even with some chromosomal changes.
Effects of Lignin-Diverted Reductant with Polyphenol Oxidases on Cellulose Degradation by Wild and Mutant Types of Lytic Polysaccharide Monooxygenase
Authors: Li Kai , Wang Yuan , Guo Xiao , Wang Bo
Published: April 2024

Establishing a multi-enzyme synergistic lignocellulosic biodegradation system using lytic polysaccharide monooxygenase (LPMO) and polyphenol oxidases is vital for efficiently utilizing plant biomass waste, ultimately benefiting the carbon cycle and promoting environmental protection. Single-residue mutations of LPMO can improve the efficiency of lignocellulosic biomass degradation. However, the activity of mutant-type LPMO in relation to lignin-diverted reducing agents has not been sufficiently explored. In this study, laccase and tyrosinase were initially investigated and their optimal conditions and impressive thermal stability were revealed, indicating their potential synergistic abilities with LPMO in lignocellulose biodegradation. When utilizing gallic acid as a reducing agent, the activities of LPMOs were increased by over 10%, which was particularly evident in mutant-type LPMOs after the addition of polyphenol oxidases. In particular, the combination of tyrosinase with either 4-hydroxy-3-methoxyphenylacetone or p-coumaric acid was shown to enhance the efficacy of LPMOs. Furthermore, the highest activity levels of wild-type LPMOs were observed with the addition of laccase and 3-methylcatechol. The similarities between wild and mutant LPMOs regarding their activities in lignin-diverted phenolic compounds and reducing agents are almost identical, suggesting that the single-residue mutation of LPMO does not have a detrimental effect on its performance. Above all, this study indicates that understanding the performance of both wild and mutant types of LPMOs in the presence of polyphenol oxidases and various reducing agents constitutes a key link in the industrialization of the multi-enzyme degradation of lignocellulose.
Enhancing Yield and Improving Grain Quality in Japonica Rice: Targeted EHD1 Editing via CRISPR-Cas9 in Low-Latitude Adaptation
Authors: Song Jian , Tang Liqun , Fan Honghuan , Xu Xiaozheng , Peng Xinlu , Cui Yongtao , Wang Jianjun
Published: April 2024

The “Indica to Japonica” initiative in China focuses on adapting Japonica rice varieties from the northeast to the unique photoperiod and temperature conditions of lower latitudes. While breeders can select varieties for their adaptability, the sensitivity to light and temperature often complicates and prolongs the process. Addressing the challenge of cultivating high-yield, superior-quality Japonica rice over expanded latitudinal ranges swiftly, in the face of these sensitivities, is critical. Our approach harnesses the CRISPR-Cas9 technology to edit the EHD1 gene in the premium northeastern Japonica cultivars Jiyuanxiang 1 and Yinongxiang 12, which are distinguished by their exceptional grain quality—increased head rice rates, gel consistency, and reduced chalkiness and amylose content. Field trials showed that these new ehd1 mutants not only surpass the wild types in yield when grown at low latitudes but also retain the desirable traits of their progenitors. Additionally, we found that disabling Ehd1 boosts the activity of Hd3a and RFT1, postponing flowering by approximately one month in the ehd1 mutants. This research presents a viable strategy for the accelerated breeding of elite northeastern Japonica rice by integrating genomic insights with gene-editing techniques suitable for low-latitude cultivation.
Insights into the Molecular Mechanism of Endothelial Glycocalyx Dysfunction during Heart Surgery
Authors: Kršek Antea , Batičić Lara , Ćurko-Cofek Božena , Batinac Tanja , Laškarin Gordana , Miletić-Gršković Silvija , Sotošek Vlatka
Published: April 2024

The endothelial glycocalyx (EGC) is a layer of proteoglycans (associated with glycosaminoglycans) and glycoproteins, which adsorbs plasma proteins on the luminal surface of endothelial cells. Its main function is to participate in separating the circulating blood from the inner layers of the vessels and the surrounding tissues. Physiologically, the EGC stimulates mechanotransduction, the endothelial charge, thrombocyte adhesion, leukocyte tissue recruitment, and molecule extravasation. Hence, severe impairment of the EGC has been implicated in various pathological conditions, including sepsis, diabetes, chronic kidney disease, inflammatory disorders, hypernatremia, hypervolemia, atherosclerosis, and ischemia/reperfusion injury. Moreover, alterations in EGC have been associated with altered responses to therapeutic interventions in conditions such as cardiovascular diseases. Investigation into the function of the glycocalyx has expanded knowledge about vascular disorders and indicated the need to consider new approaches in the treatment of severe endothelial dysfunction. This review aims to present the current understanding of the molecular mechanisms underlying cardiovascular diseases and to elucidate the impact of heart surgery on EGC dysfunction.
In Situ Synthesis of an Epoxy Resin Microwave Absorption Coating with Anti-Ultraviolet Aging Effects
Authors: Yan Shujun , Chen Xin , Zhang Angui , Tang Jun
Published: April 2024

A nanoparticle-anchored three-dimensional microsphere flower-structured layered double hydroxide (LDH) material with Fe3O4 particles was successfully prepared using simple hydrothermal and hot solvent methods. Micro-nanostructured Fe3O4@LDHs (SLF) composites balance microwave absorption, corrosion protection, and UV aging resistance. The minimum reflection loss value of SLF is −35.75 dB at 14.16 GHz, when the absorber thickness is 8 mm, and the absorption bandwidth at this frequency is up to 2.56 GHz for RL values less than −10 dB, while the LL is only 1 GHz. The SLF /EP coating has not only excellent microwave absorption performance but also excellent corrosion and UV aging resistance performance. The coating still has some anti-corrosion effect after 10 d of immersion. This work is intended as a reference for the development of new coatings with excellent microwave absorption properties as well as corrosion and UV aging resistance for wind turbine tower barrels (seaside wind power generation equipment) surfaces.
Performance Evaluation and Analysis of Urban-Suburban 5G Cellular Networks
Authors: Zreikat Aymen I. , Mathew Shinu
Published: April 2024

5G is the fifth-generation technology standard for the new generation of cellular networks. Combining 5G and millimeter waves (mmWave) gives tremendous capacity and even lower latency, allowing you to fully enjoy the 5G experience. 5G is the successor to the fourth generation (4G) which provides high-speed networks to support traffic capacity, higher throughput, and network efficiency as well as supporting massive applications, especially internet-of-things (IoT) and machine-to-machine areas. Therefore, performance evaluation and analysis of such systems is a critical research task that needs to be conducted by researchers. In this paper, a new model structure of an urban-suburban environment in a 5G network formed of seven cells with a central urban cell (Hot spot) surrounded by six suburban cells is introduced. With the proposed model, the end-user can have continuous connectivity under different propagation environments. Based on the suggested model, the related capacity bounds are derived and the performance of 5G network is studied via a simulation considering different parameters that affect the performance such as the non-orthogonality factor, the load concentration in both urban and suburban areas, the height of the mobile, the height of the base station, the radius, and the distance between base stations. Blocking probability and bandwidth utilization are the main two performance measures that are studied, however, the effect of the above parameters on the system capacity is also introduced. The provided numerical results that are based on a network-level call admission control algorithm reveal the fact that the investigated parameters have a major influence on the network performance. Therefore, the outcome of this research can be a very useful tool to be considered by mobile operators in the network planning of 5G.
Multidisciplinarity and Trandisciplinarity in the Diagnosis and Treatment of Pediatric Gastrointestinal Diseases
Authors: Mărginean Cristina Oana
Published: April 2024

It is an honor and a privilege to have helped bring this Special Issue titled “Multidisciplinarity and Trandisciplinarity in the Diagnosis and Treatment of Pediatric Gastrointestinal Diseases” to you [...]
Artificial Intelligence Provides Accurate Quantification of Thoracic Aortic Enlargement and Dissection in Chest CT
Authors: Fink Nicola , Yacoub Basel , Schoepf U. Joseph , Zsarnoczay Emese , Pinos Daniel , Vecsey-Nagy Milan , Rapaka Saikiran , Sharma Puneet , O’Doherty Jim , Ricke Jens , Varga-Szemes Akos , Emrich Tilman
Published: April 2024

This study evaluated a deep neural network (DNN) algorithm for automated aortic diameter quantification and aortic dissection detection in chest computed tomography (CT). A total of 100 patients (median age: 67.0 [interquartile range 55.3/73.0] years; 60.0% male) with aortic aneurysm who underwent non-enhanced and contrast-enhanced electrocardiogram-gated chest CT were evaluated. All the DNN measurements were compared to manual assessment, overall and between the following subgroups: (1) ascending (AA) vs. descending aorta (DA); (2) non-obese vs. obese; (3) without vs. with aortic repair; (4) without vs. with aortic dissection. Furthermore, the presence of aortic dissection was determined (yes/no decision). The automated and manual diameters differed significantly (p < 0.05) but showed excellent correlation and agreement (r = 0.89; ICC = 0.94). The automated and manual values were similar in the AA group but significantly different in the DA group (p < 0.05), similar in obese but significantly different in non-obese patients (p < 0.05) and similar in patients without aortic repair or dissection but significantly different in cases with such pathological conditions (p < 0.05). However, in all the subgroups, the automated diameters showed strong correlation and agreement with the manual values (r > 0.84; ICC > 0.9). The accuracy, sensitivity and specificity of DNN-based aortic dissection detection were 92.1%, 88.1% and 95.7%, respectively. This DNN-based algorithm enabled accurate quantification of the largest aortic diameter and detection of aortic dissection in a heterogenous patient population with various aortic pathologies. This has the potential to enhance radiologists’ efficiency in clinical practice.
Key Challenges of Cloud Computing Resource Allocation in Small and Medium Enterprises
Authors: Mohammad Abdulghafour , Abbas Yasir
Published: April 2024

Although cloud computing offers many benefits, such as flexibility, scalability, and profitability, some small and medium enterprises (SMEs) are still unable to fully utilize cloud resources, such as memory, computing power, storage, and network bandwidth. This reduces their productivity and increases their expenses. Therefore, the central objective of this paper was to examine the key challenges related to the allocation of cloud computing resources in small and medium enterprises. The method used for this study is based upon qualitative research using 12 interviews with 12 owners, managers, and experts in cloud computing in four countries: the United States of America, the United Kingdom, India, and Pakistan. Our results, based on our empirical data, show 11 key barriers to resource allocation in cloud computing that are classified based on the technology, organization, and environment (TOE) framework. Theoretically, this research contributes to the body of knowledge concerning cloud computing technology and offers valuable understanding of the cloud computing resource allocation approaches employed by small and medium enterprises (SMEs). In practice, this research is useful to aid SMEs in implementing successful and sustainable strategies for allocating cloud computing resources.
The Leisure Time Physical Activity Questionnaire for People with Disabilities: Validity and Reliability
Authors: Gee Cameron M. , Neely Ava , Jevdjevic Aleksandra , Olsen Kenedy , Martin Ginis Kathleen A.
Published: April 2024

There is an urgent need for valid and reliable measures of physical activity (PA) participation for use among people with physical and/or sensory disabilities. This study involved adapting the Leisure Time PA Questionnaire for People with Spinal Cord Injury for use in individuals with disabilities (i.e., the LTPAQ-D) and performing a preliminary evaluation of its content validity, construct validity, and same-day test–retest reliability in people with disabilities. User interviews assessed the content validity (n = 5). A cross-sectional study assessed the construct validity and same-day test–retest reliability (n = 27, 45 ± 21 years). Participants completed the LTPAQ-D, other self-report measures of aerobic and strength training PA, as well as tests of cardiorespiratory fitness (i.e., peak oxygen consumption (V˙O2peak)) and muscular strength. LTPAQ-D measures of min/week of aerobic LTPA, aerobic moderate-to-vigorous PA (MVPA), and strength training shared medium-to-large correlations with other self-report measures of aerobic and strength training PA (r = 0.458–0.942, ps < 0.01). After controlling for age, aerobic LTPA and MVPA shared moderate partial correlations with V˙O2peak (r = 0.341 and 0.356, respectively). Min/week of strength training, measured by the LTPAQ-D, was associated with predicted maximal strength on the chest press (r = 0.621, p = 0.009). All LTPAQ-D measures demonstrated good-to-excellent test–retest reliability (intraclass correlations = 0.709–0.948, ps < 0.01). This study provides preliminary evidence of the validity and reliability of the LTPAQ-D as a measure of LTPA among people with disabilities.