bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparative transcriptomic analysis of normal and abnormal in vitro flowers in Cymbidium nanulum Y. S. Wu et S. C. Chen identifies differentially expressed genes and candidate genes involved in flower formation
Authors: Fu Shuangbin , Yang Yanping , Wang Peilong , Ying Zhen , Xu Wan , Zhou Zhuang
Published: October 2022

It is beneficial for breeding and boosting the flower value of ornamental plants such as orchids, which can take several years of growth before blooming. Over the past few years, in vitro flowering of Cymbidium nanulum Y. S. Wu et S. C. Chen has been successfully induced; nevertheless, the production of many abnormal flowers has considerably limited the efficiency of this technique. We carried out transcriptomic analysis between normal and abnormal in vitro flowers, each with four organs, to investigate key genes and differentially expressed genes (DEGs) and to gain a comprehensive perspective on the formation of abnormal flowers. Thirty-six DEGs significantly enriched in plant hormone signal transduction, and photosynthesis-antenna proteins pathways were identified as key genes. Their broad upregulation and several altered transcription factors (TFs), including 11 MADS-box genes, may contribute to the deformity of in vitro flowers. By the use of weighted geneco−expression network analysis (WGCNA), three hub genes, including one unknown gene, mitochondrial calcium uniporter (MCU) and harpin-induced gene 1/nonrace-specific disease resistance gene 1 (NDR1/HIN1-Like) were identified that might play important roles in floral organ formation. The data presented in our study may serve as a comprehensive resource for understanding the regulatory mechanisms underlying flower and floral organ formation of C. nanulum Y. S. Wu et S. C. Chen in vitro.
FgMet3 and FgMet14 related to cysteine and methionine biosynthesis regulate vegetative growth, sexual reproduction, pathogenicity, and sensitivity to fungicides in Fusarium graminearum
Authors: Zhao Feifei , Yuan Zhili , Wen Weidong , Huang Zhongyu , Mao Xuewei , Zhou Mingguo , Hou Yiping
Published: October 2022

Fusarium graminearum is a destructive filamentous fungus, which widely exists in wheat and other cereal crops. Cysteine and Methionine are unique sulfur-containing amino acids that play an essential role in protein synthesis and cell life, but their functions and regulation in F. graminearum remain largely unknown. Here we identified two proteins, FgMet3 and FgMet14 in F. graminearum, which are related to the synthesis of cysteine and methionine. We found FgMet3 and FgMet14 were localized to the cytoplasm and there was an interaction between them. FgMet3 or FgMet14 deletion mutants (ΔFgMet3 and ΔFgMet14) were deficient in vegetative growth, pigment formation, sexual development, penetrability and pathogenicity. With exogenous addition of cysteine and methionine, the vegetative growth and penetrability could be completely restored in ΔFgMet3 and ΔFgMet14, while sexual reproduction could be fully restored in ΔFgMet3 and partially restored in ΔFgMet14. ΔFgMet3 and ΔFgMet14 exhibited decreased sensitivity to Congo red stress and increased sensitivity to SDS, NaCl, KCl, Sorbitol, Menadione, and Zn ion stresses. Moreover, FgMet3 and FgMet14 nonspecifically regulate the sensitivity of F. graminearum to fungicides. In conclusion, FgMet3 and FgMet14 interacted to jointly regulate the development, pathogenicity, pigment formation, sensitivity to fungicides and stress factors in F. graminearum.
Molecular basis of genetic plasticity to varying environmental conditions on growing rice by dry/direct-sowing and exposure to drought stress: Insights for DSR varietal development
Authors: Kumar Suresh , Kumar Santosh , Krishnan Gopala S. , Mohapatra Trilochan
Published: October 2022

Rice requires plenty of water for its cultivation by transplanting. This poses several challenges to its cultivation due to erratic rainfall resulting in drought, flood, and other abiotic stresses of varying intensity. Dry/direct-sown rice (DSR) has emerged as a water-saving/climate-smart alternative to transplanted rice (TPR). The performance of a rice cultivar on growing by different methods of planting under varying environmental conditions varies considerably. However, the molecular basis of the observed phenotypic plasticity of rice to varying environmental conditions is still elusive. Resilience to various environmental fluctuations is important to ensure sustainable rice production in the present era of global climate change. Our observations on exclusively up-regulated genes in leaf of Nagina 22 (N 22) grown by dry/direct-sowing and subjected to drought stress at panicle initiation stage (compared to that in leaf of IR 64), and another set of genes exclusively down-regulated in leaf of N 22 (compared to that in leaf of IR 64) indicate important roles of leaf in stress resilience. A large number of genes down-regulated exclusively in root of N 22 on dry/direct-sowing subjected to drought stress indicates a major contribution of roots in stress tolerance. The genes for redox-homeostasis, transcription factors, stress signaling, carbohydrate metabolism, and epigenetic modifications play important roles in making N 22 better adapted to DSR conditions. More importantly, the involvement of genes in rendering genetic plasticity to N 22 under changing environmental conditions was confirmed by reversal of the method of planting. To the best of our knowledge, this is the first report on decoding the molecular basis of genetic plasticity of rice grown by two different methods of planting subjected to drought stress at the reproductive stage of plant growth. This might help in DSR varietal development program to enhance water-productivity, conserve natural resources, and minimize the emission of greenhouse gases, thus achieving the objectives of negative-emission agriculture.
RopB protein of Rhizobium leguminosarum bv. viciae adopts amyloid state during symbiotic interactions with pea (Pisum sativum L.)
Authors: Kosolapova Anastasiia O. , Belousov Mikhail V. , Sulatsky Maksim I. , Tsyganova Anna V. , Sulatskaya Anna I. , Bobylev Alexander G. , Shtark Oksana Y. , Tsyganov Viktor E. , Volkov Kirill V. , Zhukov Vladimir A. , Tikhonovich Igor A. , Nizhnikov Anton A.
Published: October 2022

Amyloids represent protein aggregates with highly ordered fibrillar structure associated with the development of various disorders in humans and animals and involved in implementation of different vital functions in all three domains of life. In prokaryotes, amyloids perform a wide repertoire of functions mostly attributed to their interactions with other organisms including interspecies interactions within bacterial communities and host-pathogen interactions. Recently, we demonstrated that free-living cells of Rhizobium leguminosarum, a nitrogen-fixing symbiont of legumes, produce RopA and RopB which form amyloid fibrils at cell surface during the stationary growth phase thus connecting amyloid formation and host-symbiont interactions. Here we focused on a more detailed analysis of the RopB amyloid state in vitro and in vivo, during the symbiotic interaction between R. leguminosarum bv. viciae with its macrosymbiont, garden pea (Pisum sativum L.). We confirmed that RopB is the bona fide amyloid protein since its fibrils exhibit circular x-ray reflections indicating its cross-β structure specific for amyloids. We found that fibrils containing RopB and exhibiting amyloid properties are formed in vivo at the surface of bacteroids of R. leguminosarum extracted from pea nodules. Moreover, using pea sym31 mutant we demonstrated that formation of extracellular RopB amyloid state occurs at different stages of bacteroid development but is enhanced in juvenile symbiosomes. Proteomic screening of potentially amyloidogenic proteins in the nodules revealed the presence of detergent-resistant aggregates of different plant and bacterial proteins including pea amyloid vicilin. We demonstrated that preformed vicilin amyloids can cross-seed RopB amyloid formation suggesting for probable interaction between bacterial and plant amyloidogenic proteins in the nodules. Taken together, we demonstrate that R. leguminosarum bacteroids produce extracellular RopB amyloids in pea nodules in vivo and these nodules also contain aggregates of pea vicilin amyloid protein, which is able to cross-seed RopB fibrillogenesis in vitro. Thus, we hypothesize that plant nodules contain a complex amyloid network consisting of plant and bacterial amyloids and probably modulating host-symbiont interactions.
Identification of agro-physiological traits of lentil that reduce risks of drought
Authors: Tefera Abeya Temesgen , O’Leary Garry J. , Thayalakumaran Thabo , Rao Shiwangni , Silva-Perez Viridiana , Shunmugam Arun S. K. , Armstrong Roger , Rosewarne Garry M.
Published: October 2022

Ideotype breeding is an essential approach for selection of desired combination of plant traits for testing in crop growth model for potential yield gain in specific environments and management practices. Here we parameterized plant traits for untested lentil cultivars for the APSIM-lentil model in phenology, biomass, and seed yield. We then tested these against independent data and applied the model in an extrapolated analysis (i) to assess the impact of drought on productivity across different rainfall environments; (ii) to identify impactful plant traits and (iii) to design new lentil ideotypes with a combination of desirable traits that mitigate the impact of drought, in the context of various agronomic practices across a wide range of production environments. Desirable phenological and physiological traits related to yield were identified with RUE having the greatest effect on yield followed by HI rate. Leaf size significantly affected seed yield (p< 0.05) more than phenological phases. The physiological traits were integrated into four ideotype designs applied to two baseline cultivars (PBA Hallmark XT and PBA Jumbo2) providing eight ideotypes. We identified a combination of genetic traits that promises a yield advantage of around 10% against our current cultivars PBA Hallmark XT and PBA Jumbo2. Under drought conditions, our ideotypes achieved 5 to 25% yield advantages without stubble and 20 to 40% yield advantages with stubble residues. This shows the importance of genetic screening under realistic production conditions (e.g., stubble retention in particular environments). Such screening is aided by the employment of biophysical models that incorporate both genetic and agronomic variables that focus on successful traits in combination, to reduce the impact of drought in the development of new cultivars for various environments. Stubble retention was found to be a major agronomic contributor to high yield in water-limiting environments and this contribution declined with increasing growing season rainfall. In mid- and high-rainfall environments, the key drivers of yield were time of sowing, physiological traits and soil type. Overall, the agronomic practices, namely, early sowing, residue retention and narrow row spacing deceased the impact of drought when combined with improved physiological traits of the ideotypes based on long term climate data.
Factors influencing tourists’ shared bicycle loyalty in Hangzhou, China
Authors: Zhou Bin , Xiong Qihao , Li Ping , Wang Ling-en , Yu Hu , Jin Jianying
Published: October 2022

Focusing on Hangzhou, a famous tourist city in China, in this study, four regression models were constructed through four items of tourist loyalty to investigate the influence of tourist perceptions and characteristics on male and female tourist loyalty to shared bicycles. A questionnaire survey and ordered logistic regression model techniques were used. Survey data from 467 tourists indicated that there were significant differences between male and female tourists. For male tourists, their willingness to reuse shared bicycles (Models 1 and 2) was positively correlated with ease of access to cycles, environmental awareness, psychological benefit, and management provision; however, their willingness to recommend shared bicycles (Models 3 and 4) was only affected by environmental awareness, psychological benefit, and management provision. Among female tourists, willingness to reuse shared bicycles (Models 1 and 2) was affected by ease of access to cycles, environmental awareness, and rule adherence, while their willingness to recommend shared bicycles (Models 3 and 4) was affected by ease of access to cycles, environmental awareness, psychological benefit, and managerial provision. In addition, female tourists’ socio-demographic and behavioral characteristics had a significant impact on their loyalty, among which length of stay in Hangzhou and education were significant in the four regression models, and number of visits to Hangzhou had a positive impact on female tourists’ willingness to reuse (Models 1 and 2). In addition, female tourists who used Hellobike had higher willingness to reuse and recommend than those who used Mobike. For male tourists, only length of stay in Hangzhou had a significant impact on their reuse intention (Model 2). The current study extends the theory of attribution to explore the factors which may affect tourist’s loyalty to bicycle-sharing from the perspectives of tourists’ perceptions. It will provide further support to decision makers in the development of new shared-bicycle systems at Chinese tourist destinations, with the aim of strengthening tourist loyalty to shared-bicycle programs.
The main influencing factors of customer satisfaction and loyalty in city express delivery
Authors: Lei Zheng , Duan Huawei , Zhang Liping , Ergu Daji , Liu Fangyao
Published: October 2022

At present, customers’ low satisfaction and loyalty to city express service have restricted the development of city express. It is particularly important to analyze the factors causing customers’ low satisfaction and loyalty, which will promote the development of city express industry effectively. Based on SERVQUAL model and CCSI model, this paper constructs a new evaluation index system from the perspective of service quality. Through this new system, this paper first explores the factors that affect customers’ satisfaction and loyalty, respectively, by fuzzy analytic hierarchy process (AHP) and hierarchical regression analysis, taking the expected and perceived service quality as conversion variables. And then it analyzes the common factors that affect customers’ satisfaction and loyalty comprehensively. These two analyses will provide reference for solving the problem of low customer satisfaction and loyalty of city express enterprises. The results show that popularity and credibility, delivery time commitment, and mailing security are the common main factors affecting customer satisfaction and loyalty. Easy-to-understand receipts, the three-level index corresponding to the empathy dimension, is the most significant factor affecting customers’ loyalty in city express industry; Delivery time commitment, the three-level index corresponding to the reliability dimension, is the most significant factor affecting customers’ loyalty in city express industry.
A survey study of Chinese adolescents’ mental and interpersonal quality: Evidence from COVID-19 pandemic
Authors: Huang Leping , Zhu Yingfu , Kang Wei , Zhu Chunmu
Published: October 2022

Since 2019, the COVID-19 pandemic, as a global public health emergency, has led to stringency measures (such as lockdown) of various degrees worldwide. As these measures such as social distancing measures and mandatory lockdown are intended to minimize social mobility, they have exerted remarkable impact on individuals’ mental health, particularly, adolescents and children. The mental health problems caused include fear, anxiety, sense of isolation and development of more maladaptive behaviors due to prolonged lockdown and restricted interpersonal contact. However, well adaption status and stable interpersonal relationships play an important role in maintaining and promoting the mental health of adolescents and children. This study aims to gain a new sight of understanding of the mental health quality of Chinese adolescents during the COVID-19 Pandemic in terms of adaptation and interpersonal quality. The study is based on a survey on a total of 7,318 junior and senior high school students aged 12–18 years in various regions of China, and intended to examine the differences in adolescent mental health quality by sociodemographic variables such as gender, grade, urban and rural areas, only child, and parental education level. Our study finds that Chinese adolescents show an overall good adaptation and interpersonal quality during the lockdown. However there exists disparity across different categories in developmental patterns of adaptation and interpersonal quality. In addition, good family environment, moderate financial and emotional support, higher parental education level, etc. contribute to the cultivation and improvement of adolescents’ mental health quality. Finally, we suggest that the government and researchers should pay more attention to adolescents’ mental health issues in terms of adaption status and interpersonal relationships during the COVID-19 pandemic, especially for disadvantaged social groups such as girls, younger students, and low-income family students.
Young adult carers' identification, characteristics, and support: A systematic review
Authors: Chevrier Basilie , Lamore Kristopher , Untas Aurélie , Dorard Géraldine
Published: October 2022

Young Adult Carers (YAC) are informal carers aged 18–25 years. This is an unrecognized population. The present systematic review aims to respond to: (1) how YAC are identified in research; (2) the prevalence of YAC; (3) the characteristics of YAC; and (4) how to support YAC. Five electronic databases (Google Scholar, PsycArticle, PsycInfo, Psychology and Behavioral Sciences Collection, and PubMed) were searched for studies, scientific articles, and gray literature on YAC published prior to January 18, 2021. The quality of included studies was assessed using the Crow Critical Appraisal Tool, and a narrative method was used to underline major findings. Twenty-three studies were included and revealed that there were several ways to identify YAC, resulting in heterogeneous prevalence. Nine themes were highlighted for YAC characteristics (way into caring; care receiver; caring responsibilities; amount of caring; self-identification as a carer; living arrangement; physical, psychological, and adaptative outcomes; interpersonal relationships; education and employment); and three for YAC support (needs, available support services, and recommendations). The findings showed the diversity of YAC experiences. Although YAC expressed several needs, there are few or no support services devoted to them. More research is needed to improve political awareness.

Systematic review registration

https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42021231882, identifier: CRD42021231882.
Mapping network connectivity between internet addiction and residual depressive symptoms in patients with depression
Authors: Cai Hong , Bai Wei , Yue Yan , Zhang Ling , Mi Wen-Fang , Li Yu-Chen , Liu Huan-Zhong , Du Xiangdong , Zeng Zhen-Tao , Lu Chang-Mou , Zhang Lan , Feng Ke-Xin , Ding Yan-Hong , Yang Juan-Juan , Jackson Todd , Cheung Teris , An Feng-Rong , Xiang Yu-Tao
Published: October 2022

Background and aims

Depression often triggers addictive behaviors such as Internet addiction. In this network analysis study, we assessed the association between Internet addiction and residual depressive symptoms in patients suffering from clinically stable recurrent depressive disorder (depression hereafter).

Materials and methods

In total, 1,267 depressed patients were included. Internet addiction and residual depressive symptoms were measured using the Internet Addiction Test (IAT) and the two-item Patient Health Questionnaire (PHQ-2), respectively. Central symptoms and bridge symptoms were identified via centrality indices. Network stability was examined using the case-dropping procedure.

Results

The prevalence of IA within this sample was 27.2% (95% CI: 24.7–29.6%) based on the IAT cutoff of 50. IAT15 (“Preoccupation with the Internet”), IAT13 (“Snap or act annoyed if bothered without being online”) and IAT2 (“Neglect chores to spend more time online”) were the most central nodes in the network model. Additionally, bridge symptoms included the node PHQ1 (“Anhedonia”), followed by PHQ2 (“Sad mood”) and IAT3 (“Prefer the excitement online to the time with others”). There was no gender difference in the network structure.

Conclusion

Both key central and bridge symptoms found in the network analysis could be potentially targeted in prevention and treatment for depressed patients with comorbid Internet addiction and residual depressive symptoms.
The impact of medical service on the return behavior: A city-level study in China
Authors: Meng Mingming , Wang Zheng , Yu Ji'an
Published: October 2022

Due to the constraints of the rural-urban household registration systems, the migrants of China currently receive varying degrees of medical services. The fact that many migrants choose to return to their hometowns due to the inequality in medical care has been a social phenomenon. Using data from the 2017 China Migrant Dynamic Survey (CMDS), this paper explores the effect of medical services on population migration. Probit regression analysis method was utilized to examine the relationship between medical service level (MSL) and medical service improvement (MSI) and return behavior (RB), as well as the interaction effect between MSL and MSI, and the moderating effect of health status (HS) and health education (HE). Multiple heterogeneity tests were performed. Grouping regressions were conducted using rural household registration (RHR), grouping regressions were conducted using new rural cooperative medical system (NRCMS), and multinomial Probit regressions were conducted using migration distance and age factors. The following findings were obtained. First, when MSL is low but MSI is high in the locality of household registration, the return probability of migrants will increase. MSL also has a positive interaction effect with MSI, and they jointly increase the return probability of migrants; Second, HS and HE have a positive moderating effect on the relationships between MSL and RB and between MSI and RB; Third, heterogeneity analysis indicates that the migrants with RHR or the migrants not covered by the NRCMS are more prone to return due to the reason of medical service. In addition, the analysis also shows that middle-aged and older people who return across provinces have the highest tendency to return due to medical services and young people have the lowest propensity to return across and within provinces. The study could help local governments change their public medical care policies and close the gap between medical services in different areas. As a result, it is necessary to understand population migration trends and promote New Urbanization Strategies.
Evaluating strengths and opportunities for a co-created climate change curriculum: Medical student perspectives
Authors: Liu Irene , Rabin Benjamin , Manivannan Madhu , Laney Emaline , Philipsborn Rebecca
Published: October 2022

Introduction

Medical trainees are front-line workers in our worsening climate and health crisis. A movement is underway to teach medical students essential climate change and health content. Few evaluations of climate and health curricula exist to support ongoing curricular development, innovation, and improvement. This study explores student perspectives on climate change and health content and delivery post-implementation of a climate change and health curriculum that was co-created by students and faculty and integrated across 16 months of pre-clinical coursework at Emory University School of Medicine.

Methods

The authors conducted focus groups with the inaugural cohort of students to receive the climate and health education content at the conclusion of their preclinical curriculum. The focus groups elicited student perspectives across four domains: (i) prior perceptions of climate change and health, (ii) current attitudes about climate change and health, (iii) reflections on the existing curriculum, and (iv) opportunities for the curriculum. In this qualitative evaluation, the authors coded focus group transcripts using an inductive content analysis approach.

Results

Out of 137 eligible students in the cohort, 13 (9.5%) participated in the focus groups. Implementation strategies that students valued included contextualization and integration of climate content within existing topics and student representation through the co-creation process. Students recommended bolstering small group sessions and case-based learning to build relevant history and physical examination skills as well as creating interprofessional and community-based opportunities.

Discussion

This evaluation offers in-depth student perspectives of our climate and health curriculum. Opportunities exist to synergize climate and health education with broader transformations in medicine toward health promotion and sustainable, climate-ready healthcare. From the input of focus groups, the authors derive a framework for strengthening and extending curricular content.
Clinical features and effectiveness of Chinese medicine in patients with COVID-19 from overseas: A retrospective study in Xiamen, China
Authors: Huang Yu-Xuan , Li Na-Fen , Li Chen-Yao , Zheng Fang-Ping , Yao Xiang-Yang , Lin Bao-Hua , Huang Xian-Zhong , Zhao Neng-Jiang , Yang Jia-Yong , Chen Qiu-Min , Zhang Man-Man , Yi Li-Tao , Chen Xue-Qin
Published: October 2022

COVID-19, referred to as new coronary pneumonia, is an acute infectious disease caused by a new type of coronavirus SARS-CoV-2. To evaluate the effect of integrated Chinese medicine and Western medicine in patients with COVID-19 from overseas. Data were collected from 178 COVID-19 patients overseas at First Affiliated Hospital of Xiamen University from April 1, 2021 to July 31, 2021. These patients received therapy of integrated Chinese medicine and western medicine. Demographic data and clinical characteristics were extracted and analyzed. In addition, the prescription which induced less length of PCR positive days and hospitalization days than the median value was obtained. The top 4 frequently used Chinese medicine and virus-related genes were analyzed by network pharmacology and bioinformatics analysis. According to the chest computed tomography (CT) measurement, abnormal lung findings were observed in 145 subjects. The median length of positive PCR/hospitalization days was 7/7 days for asymptomatic subjects, 14/24 days for mild subjects, 10/15 days for moderate subjects, and 14/20 days for severe subjects. The most frequently used Chinese medicine were Scutellaria baicalensis (Huangqin), Glycyrrhiza uralensis (Gancao), Bupleurum chinense (Chaihu), and Pinellia ternata (Banxia). The putative active ingredients were baicalin, stigmasterol, sigmoidin-B, cubebin, and troxerutin. ACE, SARS-CoV-2 3CL, SARS-CoV-2 Spike, SARS-CoV-2 ORF7a, and caspase-6 showed good binding properties to active ingredients. In conclusion, the clinical results showed that integrated Chinese medicine and Western medicine are effective in treating COVID-19 patients from overseas. Based on the clinical outcomes, the putative ingredients from Chinese medicine and the potential targets of SARS-CoV-2 were provided, which could provide a reference for the clinical application of Chinese medicine in treating COVID-19 worldwide.
Finding ikigai: How robots can support meaning in later life
Authors: Randall Natasha , Joshi Swapna , Kamino Waki , Hsu Long-Jing , Agnihotri Abhijeet , Li Grace , Williamson Donald , Tsui Kate , Šabanović Selma
Published: October 2022

Previous research in human-robot interaction has explored using robots to increase objective and hedonic aspects of well-being and quality of life, but there is no literature on how robots might be used to support eudaimonic aspects of well-being (such as meaning in life). A sense of meaning has been shown to positively affect health and longevity. We frame our study around the Japanese concept ofikigai, which is widely used with Japanese older adults to enhance their everyday lives, and is closely related to the concept of eudaimonic well-being (EWB) known in Western countries. Using a mixed-methods and exploratory approach, including interviews with 17 older adults and the collection of 100 survey responses, we explored how older adults in the US experience a sense of meaning, and if and how a social robot could be used to help foster this sense. We find that meaning for older adults is often obtained by helping others, through family connections, and/or through activities of daily life, and that sources of meaning often differ based on the older adults’ living situation. Assessing how meaning compares to happiness and social connection, we highlight general similarities and differences, and also find that living situation influences older adults’ sources of happiness, desire for social connection, and barriers to well-being, in addition to companionship and happiness having a weaker correlation with meaning for those who live alone than for those who live with others. Additionally, we evaluated initial perceptions of a social robot (QT) meant to enhanceikigaiand overall well-being, finding mostly positive perceptions, though those who live alone also reported being less willing to adopt a social robot into their homes. Using both data collected on older adults’ meaning and the potential use of QT to support meaning, we make several design recommendations with regards to using robots to enhanceikigai, such as by prompting daily reflecting, enhancing family bonds, and suggesting new experiences and volunteer opportunities.
Bio-optical properties of the Brazilian Abrolhos Bank’s shallow coral-reef waters
Authors: Medeiros Thais Andrade Galvão , Zoffoli María Laura , Frouin Robert , Cortivo Fábio Dall , Cesar Gabriel Moiano , Kampel Milton
Published: October 2022

The Abrolhos Bank harbors the richest coral reef ecosystem in the South Atlantic Ocean. It exhibits unique geomorphologic structures, is localized in shallow depths, and is divided into two reef regions with an inner arc close to the coast (3–20 m depth) and an outer deeper arc (5–30 m depth). This study aims to describe some bio-optical properties of the Abrolhos Bank waters and to evaluate the performance of the inversion Hyperspectral Optimization Processing Exemplar (HOPE) model, developed to retrieve optical properties in shallow waters, in the region. To this end, measurements at 75 stations during two field campaigns conducted during the 2013 and 2016 wet seasons were analyzed, and the HOPE model was applied to both in situ remote sensing reflectance (Rrs) spectra and PRecursore IperSpettrale della Missione Applicativa (PRISMA) imagery. Significant differences in optical and biological properties were found between the two arcs. The empirical relationships between chlorophyll-a concentration (Chl-a) and absorption coefficient of phytoplankton at 440 nm (aphy(440)) diverged from Bricaud’s models, suggesting differences in phytoplankton diversity and cell size. In both arcs, total non-water absorption coefficient at 440 nm (aT-w(440)) was dominated by colored dissolved organic matter (CDOM) by ∼60%. Absorption coefficient by CDOM (acdom) presented a higher variability within the outer arc, with the lowest contribution from non-algal particles (NAPs), and the spectral slopes of aCDOM resembled those of the inner arc. The spectral slopes of the NAP absorption coefficient suggested a dominance by organic rather than mineral particles that probably originated from biological production. The HOPE model applied to in situ Rrs performed satisfactorily for depth in the Abrolhos Bank waters, although retrievals of aphy(440), CDOM plus NAP (adg(440)) and aT-w(440) were underestimated with a relative bias of −27.9%, −32.1% and −45.8%, respectively. The HOPE model retrievals from the PRISMA image exhibited low aphy(440) values over the whole scene and the highest adg(440) values in the Caravelas river plume. Very shallow depths (≤3 m), bottom substrate reflectance used as input in the HOPE model, model parametrization associated with the water complexity in the study site, and uncertainties associated to Rrs measurements used as input might be responsible for differences found when comparing HOPE retrievals with in situ measurements.
Linking remote sensing, in situ and laboratory spectroscopy for a Ryugu analog meteorite sample
Authors: Maturilli Alessandro , Schwinger Sabrina , Bonato Enrica , Helbert Jörn , Baqué Mickael , Hamm Maximilian , Alemanno Giulia , D’Amore Mario
Published: October 2022

In 2022 JAXA issued an Announcement of Opportunity (AO) for receiving Hayabusa2 samples returned to Earth. We responded to the AO submitting a proposal based on using a multi-prong approach to achieve two main goals. The first goal is to address the subdued contrast of remote-sensing observations compared to measurements performed under laboratory conditions on analog materials. For this we will link the hyperspectral and imaging data collected from the spacecraft and the in-situ observations from the MASCOT lander instruments (MARA and MASCam) with laboratory-based measurements of Hayabusa2 samples using bi-directional reflectance spectroscopy under simulated asteroid surface conditions from UV to MIR/FIR achieved using three Bruker Vertex 80 V spectrometers in the Planetary Spectroscopy Laboratory. The second goal is the investigation of the mineralogy and organic matter of the samples collected by Hayabusa2, to better understanding the evolution of materials characterizing Ryugu and in general of protoplanetary disk and organic matter, investigating the aqueous alteration that took place in the parent body, and comparing the results with data collected from pristine carbonaceous chondrite analog meteorites. Spectral data will be complemented by Raman spectroscopy under simulated asteroid surface conditions, X-ray diffraction, would also allow us to define the bulk mineralogy of the samples as well as investigate the presence and nature of organic matter within the samples. In situ mineralogical and geochemical characterization will involve a pre-characterization of the sample fragments through scanning electron microscopy low voltage electron dispersive X-ray (EDX) maps, and micro IR analyses of the fragments. If allowed, a thin section of one grain will be used for electron microprobe analyses to geochemically characterize its mineralogical composition. To train our data collection and analysis methods on a realistic sample, we selected a piece of the Mukundpura meteorite, as one of the closer analogs to Ryugu’s surface (Ray et al., Planetary and Space Science, 2018, 151, 149–154). The Mukundpura chunk we selected for this study measures 3 mm in its maximum dimension, and we chose it so to have a test sample of the same size as the Hayabusa2 grain we requested in our proposal to JAXA’s AO. The test gave us confidence that we can measure with good SNR measurements in bi-directional reflectance for samples around 3 mm in size (see Figures 3, 4 below). To address our second goal the spectral data was complemented by Raman spectroscopy measured again under simulated asteroid surface conditions in our Raman Mineralogy and Biodetection Laboratory at DLR.
Can Computers Understand Humor?
Authors: Shani Chen , Shahaf Dafna
Published: October 2022

Computers are very good at performing many tasks that are difficult for humans, such as solving complicated math problems or predicting the weather. But there are many things that people do better than computers. Humor—that is, telling jokes and knowing when something is funny—is one of the things that computers cannot do as well as humans. In this article, you will learn why it is hard for computers to understand jokes. You will also learn about artificial intelligence (AI), and how a new sub-field of AI, called machine learning, can help computers understand humor. We hope that, in the future, computers will be able to understand and generate humor, just like humans do—or even better!
Factors for selective ultrasound screening in newborns with developmental dysplasia of the hip (DDH)
Authors: Angsanuntsukh Chanika , Patathong Tanyaporn , Klaewkasikum Krongkaew , Jungtheerapanich Witoon , Saisongcroh Tanyawat , Mulpruek Pornchai , Woratanarat Patarawan
Published: October 2022

Background

Hip ultrasound screening for DDH provides better sensitivity compared to physical examination. Due to a lower prevalence and limited resources, selective hip ultrasound in newborns at risk could be considered a proper screening protocol in Thailand and Asian countries.

Objective

This study was aimed to evaluate risk factors and define criteria for selective screening.

Methods

A case-control study was conducted in 2020. All newborns with hip ultrasound screening were included. Cases were defined as newborns with abnormal hip ultrasounds, while controls were those with normal studies. Inter and intra-rater reliability were evaluated. All factors were analyzed using univariate and multivariate logistic regression. The model performance was tested by Hosmer-Lemeshow goodness of fit. Internal validity was performed by the split data method. Area under the receiver operating characteristic (ROC) curve was estimated.

Results

Ninety-five newborns (29 cases and 66 controls) were included. Eighty percent of cases and 58% of controls were female. The gestational age was 36.6 and 37.7 weeks in case and control, respectively. Female, breech presentation, positive Ortolani test, positive Barlow test, and limited hip abduction were significant factors with odds ratio of 2.82, 5.12, 34.21, 69.64, and 5.48, respectively. The final model included breech presentation, positive Ortolani test, and positive Barlow test. The model cut-off value 15.02 provided sensitivity (93.10%) and specificity were (80.30%). The area under the ROC curve was 0.9308. The split data remained significant internal validity for all factors with p-value < 0.05.

Conclusion

Careful history taking and physical examination are essential to identify the risk factors for DDH. Newborns with breech presentation, positive Ortolani test and positive Barlow test should be screened by hip ultrasound.
Endoscopic treatment for calcific tendinitis of the gluteus medius: A case report and review
Authors: Jian Chen , Dan Wang , Gangliang Wang
Published: October 2022

Tendon calcification is a common disease, with the shoulder rotator cuff being the most common site. However, calcific tendinitis of the gluteus medius has rarely been reported. This study reports the case of a 64-year-old woman diagnosed with calcific tendinitis of the gluteus medius and experiencing right lateral hip pain with no apparent trigger. After unsuccessful conservative treatment, hip endoscopy was performed on this patient, allowing for a clear view of a “toothpaste-like” lesion in the gluteus medius tendon. A shaver was used to remove the lesion. After 8 weeks and 14 months of follow-up, the patient could return to regular daily and social activities. This study shows that endoscopic surgery can lead to effective, rapid recovery and minimally invasive clinical outcomes in patients with tendon calcification near the hip joint.
Watch your language: An exploration of the use of causal wording in veterinary observational research
Authors: Sargeant Jan M. , O'Connor Annette M. , Totton Sarah C. , Vriezen Ellen R.
Published: October 2022

Observational research may be conducted to predict an outcome or to identify associations between an intervention or risk factor (an “exposure”) and an outcome. However, the end goal of observational research often is to identify exposures that can be manipulated to improve an outcome, meaning that the aim is identify causal relationships. Causal inference from observational studies may be appropriate when an exposure-outcome of interest is identified, causal reasoning is used to identify confounders, confounders are adequately controlled, and theoretical issues, such as temporality, are considered. If these conditions are not met, causal inference cannot be made in an observational study. The objective of our study was to explore the use of causal language in veterinary observational studies, and to compare the use of causal language between studies that appear to be predictive or associational in purpose vs. those that appear to be exploring causal relationships. The dataset comprised 200 observational studies in veterinary species published between 2020 and 2022. The majority (117 out of 200) were cross-sectional studies. There were 48 studies that described an exposure-outcome of interest, and we considered these studies to be exploring potential causal relationships; of note, this liberal categorization would be anticipated to overestimate the proportion of studies suitably designed for causal inference. Overall, 172 studies (86%) used causal wording in at least one section of the article. Causal language was used in 128/152 (84%) of studies exploring predictions or associations; this language implies causation when it is not appropriate to do so. In studies designed such that causal inference might be possible, 44/48 (92%) used causal language in one or more sections. There were no substantive differences in the use of causal wording between observational study designs, exposure types, or whether the first author's affiliation was a country in which English is an official language. There is a need for authors of veterinary observational studies to explicitly state the purpose of the study (associational, predictive, or causal), and to use causal wording appropriately based on the aim of the study.
Longitudinal assessment of competency development at The Ohio State University using the competency-based veterinary education (CBVE) model
Authors: Read Emma K. , Maxey Connor , Hecker Kent G.
Published: October 2022

With the development of the American Association of Veterinary Medical Colleges' Competency-Based Veterinary Education (CBVE) model, veterinary schools are reorganizing curricula and assessment guidelines, especially within the clinical rotation training elements. Specifically, programs are utilizing both competencies and entrustable professional activities (EPAs) as opportunities for gathering information about student development within and across clinical rotations. However, what evidence exists that use of the central tenets of the CBVE model (competency framework, milestones and EPAs) improves our assessment practices and captures reliable and valid data to track competency development of students as they progress through their clinical year? Here, we report on validity evidence to support the use of scores from in-training evaluation report forms (ITERs) and workplace-based assessments of EPAs to evaluate competency progression within and across domains described in the CBVE, during the final year clinical training period of The Ohio State University's College of Veterinary Medicine (OSU-CVM) program. The ITER, used at the conclusion of each rotation, was modified to include the CBVE competencies that were assessed by identifying the stage of student development on a series of descriptive milestones (from pre-novice to competent). Workplace based assessments containing entrustment scales were used to assess EPAs from the CBVE model within each clinical rotation. Competency progression and entrustment scores were evaluated on each of the 31 rotations offered and high-stakes decisions regarding student performance were determined by a collective review of all the ITERs and EPAs recorded for each learner across each semester and the entire year. Results from the class of 2021, collected on approximately 190 students from 31 rotations, are reported with more than 55 299 total competency assessments combined with milestone placement and 2799 complete EPAs. Approximately 10% of the class was identified for remediation and received additional coaching support. Data collected longitudinally through the ITER on milestones provides initial validity evidence to support using the scores in higher stakes contexts such as identifying students for remediation and for determining whether students have met the necessary requirements to successfully complete the program. Data collected on entrustment scores did not, however, support such decision making. Implications are discussed.
An open secret in porcine acute myocardial infarction models: The relevance of anaesthetic regime and breed in ischaemic outcomes
Authors: Solanes Núria , Bobi Joaquim , Arrieta Marta , Jimenez Francisco Rafael , Palacios Carmen , Rodríguez Juan José , Roqué Mercè , Galán-Arriola Carlos , Ibañez Borja , Freixa Xavier , García-Álvarez Ana , Sabaté Manel , Rigol Montserrat
Published: October 2022

Large animal models of acute myocardial infarction (AMI) play a crucial role in translating novel therapeutic approaches to patients as denoted by their use in the right-before-human testing platform. At present, the porcine model of AMI is used most frequently as it mimics the human condition and its anatomopathological features accurately. We want to describe to, and share with, the translational research community our experience of how different anaesthetic protocols (sevoflurane, midazolam, ketamine+xylazine+midazolam, and propofol) and pig breeds [Large White and Landrace x Large White (LLW)] can dramatically modify the outcomes of a well-established porcine model of closed-chest AMI. Our group has extensive experience with the porcine model of reperfused AMI and, over time, we reduced the time of ischaemia used to induce the disease from 90 to 50 min to increase the salvageable myocardium for cardioprotection studies. For logistical reasons, we changed both the anaesthetic protocol and the pig breed used, but these resulted in a dramatic reduction in the size of the myocardial infarct, to almost zero in some cases (sevoflurane, 50-min ischaemia, LLW, 2.4 ± 3.9% infarct size), and the cardiac function was preserved. Therefore, we had to re-validate the model by returning to 90 min of ischaemia. Here, we report the differences in infarct size and cardiac function, measured by different modalities, for each combination of anaesthetic protocol and pig breed we have used. Furthermore, we discuss these combinations and the limited literature pertaining to how these two factors influence cardiac function and infarct size in the porcine model of AMI.