bluuebunny/crossref_metadata_2025 huggingface dataset:
Edukasi protokol Isolasi Mandiri dan penyaluran bantuan keluarga terdampak pandemi Dikecamatan Luwuk
Authors: Said Rugayah , Hasan Sri Musriniawati , Subchan Djadid , Mangemba Dg.
Published: January 2024

Covid-19 telah dinyatakan sebagai pandemic dunia oleh WHO, Dikabupaten Banggai kasus konfirmasi Covid- 19 Sebanyak 7.175 Jiwa terdiri dari 6858 orang Sembuh dan 317 Meninggal dunia, di Kecamatan Luwuk jumlah Kasus Konfirmasi Covid – 19 berjumlah 1.095 Jiwa.Penanganan Pasien Covid selain di Rawat di Rumah Sakit dilakukan Isolasi Mandiri di Rumah. Tujuan Kegiatan meningkatkan pengetahuan dan Pemahaman kepada pasien dan keluarga dalam  melakukan perawatan mandiri saat isolasi mandiri sesuai standar operasional prosedur PAPDI Perhimpunan Dokter Spesialis Penyakit Dalam Indonesia dan meningkatkan asupan gizi pasien terdampak pandemi. Kegiatan dilaksanakan pada tanggal 03 s/d 05 Agustus 2021.Pasien yang terdampak Covid-19 menerima dengan baik Informasi yang diberikan melalui Saluran telepon Seluler dan keluarga menerima bantuan Sembako yang diberikan, pelaksanaan Kegiatan berjalan dengan lancer, kegiatan ini sangat penting  merupakan salah satu tindakan memutus mata rantai penularan Covid – 19  dikeluarga dan masyarakat umumunya.
Edukasi Penerapan Protokol Kesehatan dalam Upaya Pencegahan Penularan Covid-19 di Kelurahan Pantoloan
Authors: Lisnawati Lisnawati , Zainul Zainul , Muliani Muliani , Hafid Fahmi , Taqwin Taqwin
Published: January 2024

Introduction: In 2021 there were 22,403 positive confirmed cases of Covid-19 in Central Sulawesi, 595 cases died, and 5,992 people underwent quarantine. The number who have confirmed positive in Palu City is 4,993 people. One of the areas at high risk of an increase in cases in Palu City is Pantoloan Village, which is a port area where passengers come and go from various regions. Health cadres and related organizations need to be empowered to increase socialization of the implementation of health protocols in the community to prevent Covid-19. Community service program activities by lecturers at the Poltekkes Kemenkes Palu aim to provide understanding to the public about the application of health protocols in an effort to prevent the transmission of Covid-19 in the Pantoloan Village. The methods of this activity is carried out by providing counseling and education to implement health protocols so that the transmission of Covid-19 can be prevented with infocus media. Implementation of activities on July 12-13 2022. Participants in this activity totaled 30 people consisting of PKK cadres and mothers. The results show an increase in knowledge from the pre and post test with an average score of 60.80 to an average of 80.90. Suggestions for PKK cadres and women who have been educated together with the local government and community leaders to actively carry out health socialization or promotion related to the implementation of correct and routine health protocols, especially in the Pantoloan Village. For related agencies, it is necessary to educate the public regarding the spread of Covid-19, especially in areas prone to spread, such as in port and border areas.
Pelatihan Deteksi Neuropati Dengan Teknik Ipswich Touch Test pada Pasien Diabetes Melitus
Authors: Aminuddin Aminuddin , Baiq Emy Nurmalisa , Hasbunsyah Siregar
Published: January 2024

Introduction: Diabetic neuropathy is a complication of diabetes that occurs quite often with a percentage of 28.7-39.5%. One of the efforts to reduce this number is early detection, which can be done by the general public. Early detection using the IpTT method has been shown to be safe, simple, requires no equipment, and can be done publicly. The purpose of this social service is to train health cadres to detect diabetic neuropathy using the IpTT method. Methods The service team implements this public service activity by applying the methods of lecture, discussion, demonstration and face-to-face training among the participants. This activity took place on Saturday, July 10, 2021, in the nursing class D3 of the Palus Tervishoiu Professional University. The target group of this activity is 36 health cadres representing 18 posbindus. Results: After the training and IpTT preparatory demonstration, the mean posttest score was 80 and was good in the IpTT preparatory skill category. Due to these results, it is necessary to periodically conduct IpTT trainings to update and improve the knowledge and skills of the cadres in conducting IpTT examinations. This educational activity using IPTT technology to detect diabetic neuropathy can be used as one program in the Talis Health Center area to reduce the number of amputations in diabetic patients.
Edukasi Perawatan Kaki Diabetes kepada Kader Kesehatan
Authors: Sasmita Hanum , Nurmalisa Baiq Emy , Pangaribuan Helena
Published: January 2024

Introduction Central Sulawesi is ranked 10th with 2.2% of sufferers. If optimal prevention is not carried out from the start, the wound will deteriorate rapidly, and the worst is amputation. One of the efforts that can be made to prevent DFU from occurring is to do foot care. Foot care in people with DM is an attempt to avoid disturbances in the feet which, if continued, can lead to amputation. The goal to be achieved from this community service activity is to increase the knowledge of cadres in conducting education about Foot Care for Patients with Diabetes Mellitus in the Working Area of the Talise Health Center. The method used in this activity is a combination of lecture, discussion, demonstration and practice methods. The media used to help convey material is a leaflet. The activity begins with the preparation, implementation and evaluation stages. To find out the effectiveness of this activity, an apperception was carried out to measure the level of knowledge of the participants and at the end of the activity an evaluation was carried out by asking questions related to the material that had been presented and participants were asked to demonstrate DM foot care. Results The level of success in implementing this community service can be seen from the achievement of the number of participants, objectives and material targets. The number of participants who attended were 20 participants. Conclusion The purpose of the dedication was achieved, seen from the enthusiasm of the participants and at the end of the activity the participants were able to answer questions posed by the service team and were able to answer questions related to DM foot care. Suggestion It is hoped that the Talise Health Center will carry out similar activities on a regular basis and a larger number of participants so that all DM patients in the Talise Health Center work area have good knowledge and skills in performing foot care.
Fungsi Pemeliharaan Kesehatan Keluarga Terhadap Orang Dengan Gangguan Jiwa di Kecamatan Marawola Kabupaten Sigi
Authors: Saifah Andi , Febriyanti Dahlia
Published: January 2024

Salah satu indikator Program Indonesia Sehat Pendekatan Keluarga (PIS-PK) adalah penderita gangguan jiwa mendapat pengobatan dan tidak ditelantarkan.  Tujuan Penelitian adalah mengidentifikasi tugas kesehatan keluarga terhadap orang dengan gangguan jiwa (ODGJ). Desain Penelitian adalah penelitian deskriptif desain survey.  Populasi adalah semua keluarga dengan ODGJ, menggunakan total sampling sebanyak 18 keluarga. Alat pengumpulan data adalah kuesioner dengan 35 pernyataan terkait lima kesehatan keluarga terhadap ODGJ. Analisis data yang digunakan adalah analisis univariat (distribusi frekuensi). Hasil penelitian adalah tugas kesehatan keluarga terhadap ODGJ kategori baik sebanyak 10 keluarga (55,56%), kategori tidak baik sebanyak 8 keluarga (44,44%) khususnya dalam pemanfaatan pelayanan kesehatan masih rendah yaitu kontrol dan pengobatan tidak teratur sebanyak 7 keluarga (87,5%). Kesimpulan penelitian adalah keluarga belum maksimal dalam pemeliharaan kesehatan anggota keluarganya yang mengalami gangguan jiwa di Kecamatan Marawola. Rekomendasi penelitian adalah peningkatan asuhan keperawatan keluarga melalui Program PERKESMAS Puskesmas Marawola.
The Effects Of Education On The Management Of Drowning Victims On Increasing Knowledge Of Coastal Communities In Tompotana Village Takalar Regency
Authors: Mangemba Dg , Ra’bung Alfrida Semuel , Nurarifah Nurarifah , Ismunandar Ismunandar , Ismunandar Ismunandar , Abdul Malik Supriadi , Junaidi Junaidi , Metrys Ndama  , Yoan Maria Agusta  , Nasrul
Published: January 2024

Masalah tenggelam dapat ditanggulangi dengan meningkatkan pengetahuan masyarakat pesisir tentang penanganan awal korban tenggelam dari sumber yang terpercaya seperti tenaga kesehatan tentang tekhnik pertolongan pertama pada korban tenggelam. Penelitian ini bertujuan untuk mengetahui pengaruh pendidikan penanganan korban tenggelam terhadap peningkatan pengetahuan masyarakat pesisir. Jenis penelitian ini adalah kuantitatif dengan metode Pra Eksperimen dengan Desain One Group Pretest-Posttest. Teknik pengambilan sampel menggunakan Purposive Sampling. Besar sampel sebanyak 52 orang yang berada dan tinggal di Dusun Tompotana yang memenuhi kriteria sampel. Hasil penelitian dianalisis dengan uji Wilcoxon menggunakan analisa bivariate. Hasil penelitian menunjukkan bahwa berdasarkan nilai perbedaan yang signifikan antara sebelum dan sesudah diberikan pendidikan penanganan korban tenggelam dapat diketahui dari nilai Pvalue = 0,001. Hal tersebut menunjukkan adanya pengaruh pendidikan penanganan korban tenggelam terhadap peningkatan pengetahuan masyarakat pesisir. Kesimpulan dari penelitian ini yaitu terdapat pengaruh yang signifikan antara pendidikan penanganan korban tenggelam tehadap peningkatan pengetahuan masyarakat pesisir di Dusun Tompotana Desa Tompotana.
Pengaruh Diabetes Self Management Education Terhadap Perubahan Kadar Gula Darah Pada Penderita Diabetes Melitus Tipe II di Wilayah Kerja Puskesmas Nanga Kecamatan Lela Kabupaten Sikka
Authors: Avelina Yuldensia , Pangaribuan Helena , Anjelina Yeri Sisilia
Published: January 2024

ABSTRAK

Latar Belakang: Jumlah penderita DM di Indonesia menempati urutan ke-4 terbesar di dunia. Keberhasilan pengelolaan DM tergantung pada manajemen diri pasien untuk mengontrol gejala dan menghindari komplikasi. Manajemen diri yang tinggi dapat meningkatkan kualitas hidup pasien DM tipe 2 dan dapat mengendalikan kadar gula darah dalam batas normal. Tujuan: Tujuan penelitian ini adalah untuk mengidentifikasi pengaruh antara Diabetes Self Management Education terhadap perubahan kadar gula darah pada penderita Diabetes Melitus tipe 2 di wilayah kerja Puskesmas Nanga Kecamatan Lela Kabupaten Sikka. Metode: Jenis penelitian yang digunakan dalam penelitian ini adalah quasi eksperimen dengan rancangan one group pretest posttest design. Populasi pada penelitian ini adalah semua penderita DM Tipe 2 di wilayah kerja Puskesmas Nanga sebanyak 32 responden. Teknik sampel yang digunakan yaitu total sampling dengan alat pengambilan data menggunakan glukometer dan analisis data menggunakan uji Marginal Homogeneity. Hasil: Hasil penelitian didapatkan p value = 0,000 ˂ α (0.05) sehingga dapat disimpulkan bahwa ada pengaruh antara Diabetes Self Management Education (DSME) terhadap perubahan kadar gula darah pada penderita Diabetes Melitus tipe II di Wilayah Kerja Puskesmas Nanga Kecamatan Lela Kabupaten Sikka. Pasien dapat melakukan pengelolaan manajemen diri sehingga dapat membantu penurunan kadar gula darah.
Relationship between Mother's Knowledge about Exclusive Breastfeeding and Giving MPASI with Stunting Incidence in Children Aged 24-59 Months in Bale Village Working Area of Wani Health Center, Donggala District
Authors: Nurmalisa Baiq Emy
Published: January 2024

Stunting is a condition in which toddlers have less length or height when compared to their age. Globally in 2016 as many as 22.9% or around 154.8 million children under five in the world suffer from stunting. The number of stunting cases in toddlers in Bale Village was 58 children. The aim of the study was to find out the relationship between mother's knowledge about exclusive breastfeeding and giving MP-ASI with the incidence of stunting in toddlers aged 24-59 months in Bale Village, working area of ​​the Wani Health Center, Tanantovea District, Donggala Regency. Methods This type of research is a correlation analytic method with a cross sectional design. The population in this study were all mothers with toddlers aged 24-59 months in Bale Village totaling 188 populations. The number of samples is 53 samples, with the sampling technique proportional random sampling. Data analysis used the chi-square test. The results showed that there was a relationship between mothers' knowledge about exclusive breastfeeding and the incidence of stunting in toddlers aged 24-59 months in Bale Village, Working Area of ​​the Wani Public Health Center, Tanantovea District, Donggala Regency (p-value = 0.001) and there was a relationship between complementary feeding and stunting. in toddlers aged 24-59 months in Bale Village, Working Area of ​​Wani Public Health Center, Tanantovea District, Donggala Regency (p-value = 0.000). The conclusion of this study is that there is a relationship between mother's knowledge about exclusive breastfeeding and giving MP-ASI with the incidence of stunting in toddlers aged 24 -59 months in Bale Village working area of ​​the Wani Health Center, Tanantovea District, Donggala Regency. It is hoped that the Wani Health Center will routinely carry out health counseling and provide nutritious MP-ASI so that it can prevent stunting in toddlers.
Pengaruh Guide Imagery Relaxation terhadap Tingkat Nyeri pada Pasien Fraktur yang dilakukan Tindakan Pembedahan di Ruang Paviliun Teratai RSUD Undata Palu
Authors: Ndama Metyrs , Ismunandar Ismunandar
Published: January 2024

Latar Belakang: Gejala fraktur yang paling umum adalah rasa sakit, pembengkakan dan kelainan bentuk. Rasa sakit akan bertambah berat dengan gerakan dan penekanan diatas fraktur. Salah satu tindakan keperawatan untuk mengatasi nyeri adalah tindakan guide imagery relaxation. Berdasarkan wawancara dengan perawat, tehnik ini jarang dilakukan sementara tehnik ini mudah dan praktis dalam pelaksanaan. Tujuan penelitian ini diketahuinya pengaruh guide imagery relaxation terhadap tingkat nyeri pada pasien fraktur yang dilakukan tindakan pembedahan di ruang pavilion Teratai RSUD Undata Palu. Metode Penelitian ini adalah  penelitian quasi eksperimen dengan desain pretest dan posttest group design. Populasi adalah pasien fraktur yang dilakukan tindakan pembedahan, besar sampel adalah 15 orang yang dihitung dengan menggunakan rumus estimasi proporsi populasi tidak diketahui. Cara pengambilan sampel adalah purposive sampling. Pengolahan data menggunakan fasilitas komputer, análisis yang digunakan adalah análisis univariat dan análisis bivariat dengan menggunakan uji T Dependen dengan tingkat kemaknaan 0,05. Hasil penelitian diperoleh sebelum dilakukan tindakan guide imagery relaxation penderita yang mengalami nyeri kategori sedang lebih banyak yaitu 86,6% den mean rata rata adalah 5,53. Setelah dilakukan tindakan guide imagery relaxation 100 % pasien mengalami nyeri dengan kategori ringan dan mean rata rata 2,867. Hasil uji T Dependen diperoleh nilai p = 0,000 (p < 0,05). Kesimpulan penelitian ini ada pengaruh tindakan guide imagery relaxation terhadap tingkat nyeri pada pasien fraktur yang dilakukan tindakan pembedahan (H0 di tolak).  Disarankan kepada perawat di RSUD Undata Palu untuk dapat mengajarkan dan menerapkan tindakan keperawatan ini pada pasien yang mengalami masalah keperawatan nyeri.
Pengaruh Pendidikan Kesehatan Menggunakan Media Video Animasi Terhadap Kesiapan dan Pengetahuan dalam Menghadapi Menarche
Authors: Sari Diah Wulan , Hardiyanti Diana , Pertiwi Melinda Restu
Published: January 2024

Background: Menarche (first menstruation) is the culmination of a series of primary and secondary changes that occur in young women. Menarche will be a traumatic event for some young women who are not prepared for it. Lack of information and knowledge about reproductive health, especially menstruation affects adolescents' perceptions of menarche. Purpose: : to determine the effect of Health Education using animated video media on readiness in dealing with menarche at SDN Jawa 5 Martapura. Methods: this research is quantitative with a pre-experimental research design type of one group pretest-posttest design. Results: this study shows that there is an effect of health education using animated video media on readiness in facing menarche at SDN Jawa 5 Martapura with a p-value of 0.001 < (0.05). Conclusion: it can be seen that there is an effect of health education using animated video media on readiness in facing menarche. is expected to increase knowledge and insight for respondents regarding readiness in facing menarche. One way to increase preparedness in facing menarche is to increase knowledge through the provision of health education about menstruation
Analisis Determinan Faktor yang Berhubungan dengan Restless Leg Syndrome pada Pasien yang Menjalani Hemodialisa
Authors: Nurmalisa Baiq Emy , Pangaribuan Helena , Siregar Hasbunsyah
Published: January 2024

Latar Belakang: Terapi hemodialisis dapat membantu pasien gagal ginjal kronik akan tetapi disisi lain terapi ini juga dapat menimbulkan resiko komplikasi termasuk terjadinya komplikasi neurologi yaitu Restless Leg Syndrome (RLS).Tujuan dalam penelitian ini adalah menganalisis faktor-faktor yang berhubungan dengan Restless Leg syndrome pada pasien hemodialisa.  Metode: Penelitian ini merupakan penelitian analitik dengan pendekatan crosssectional. Sampel penelitian ini berjumlah 58 orang pasien hemodialisa yang diambil dengan teknik total sampling. Penelitian ini dilaksanakan di ruang hemodialisa RSUD Anutapura Palu.Instrumen yang digunakan adalah  kuesioner Restless Legs Syndrome Rating Scale versi bahasa Indonesia dan kuesioner demografi.  Data dalam penelitian ini dianalisis menggunakan analisis univariat dan bivariat dengan uji spearman, uji chi square.  Hasil: Sebagian besar  responden memiliki derajat restless leg sindrom kategori sedang sebanyak 26 orang (44,8%). Terdapat hubungan bermakna antara umur dengan RLS (p=0,042), jenis kelamin dengan RLS (0,019), lama HD dengan RLS (p=0,023), jumlah penyakit penyerta dengan  dengan RLS (0,030), dan kadar haemoglobin dengan RLS (p=0,023). Saran Studi lebih lanjut diperlukan penelitian multicenter dan sampel yang lebih luas serta intervensi untuk mengurangi kejadian RLS pada pasien hemodialisa.
Pneumonia and COVID-19 co-infection modeling with optimal control analysis
Authors: Aga Beza Zeleke , Keno Temesgen Duressa , Terfasa Debela Etefa , Berhe Hailay Weldegiorgis
Published: January 2024

In this study, we present a nonlinear deterministic mathematical model for co-infection of pneumonia and COVID-19 transmission dynamics. To understand the dynamics of the co-infection of COVID-19 and pneumonia sickness, we developed and examined a compartmental based ordinary differential equation type mathematical model. Firstly, we showed the limited region and non-negativity of the solution, which demonstrate that the model is biologically relevant and mathematically well-posed. Secondly, the Jacobian matrix and the Lyapunov function are used to illustrate the local and global stability of the equilibrium locations. If the related reproduction numbers R0c, R0p, and R0 are smaller than unity, then pneumonia, COVID-19, and their co-infection have disease-free equilibrium points that are both locally and globally asymptotically stable otherwise the endemic equilibrium points are stable. Sensitivity analysis is used to determine how each parameter affects the spread or control of the illnesses. Moreover, we applied the optimal control theory to describe the optimal control model that incorporates four controls, namely, prevention of pneumonia, prevention of COVID-19, treatment of infected pneumonia and treatment of infected COVID-19. Then the Pontryagin's maximum principle is introduced to obtain the necessary condition for the optimal control problem. Finally, the numerical simulation of optimality system reveals that the combination of treatment and prevention is the most optimal to minimize the diseases.
Effect of precursor morphology of cellulose-based hard carbon anodes for sodium-ion batteries
Authors: Sarma Hridip Ranjan , Sun Ju , Gunathilaka Isuru E. , Hora Yvonne , Rajkhowa Rangam , Forsyth Maria , Byrne Nolene
Published: January 2024

Hard carbon with different microstructures and physicochemical properties can be obtained based on the precursor used, and these properties have a direct impact on the electrochemical performance. Herein, two different precursors from a single source of waste cotton textiles have been prepared to be either cotton snippets retaining the original fiber structure of cotton or a microfibrillated cellulose, which has a very different morphology and surface area. Both the cotton snippet (CS) and the microfibrillated cellulose (MFC) have been carbonized to prepare hard carbons MFC-C and CS-C, and their electrochemical performance is evaluated in sodium-ion batteries (NIBs). Physicochemical properties in terms of a higher interlayer spacing of 3.71 Å and a high defect ratio (ID/IG) of 1.10 resulted in CS-C having a relatively higher specific capacity of 240 mAh g-1 in comparison to 199 mAh g-1 in MFC-C when cycled at 50 mA g-1. In addition, ex-situ MAS (magic angle spinning) NMR (nuclear magnetic resonance) spectroscopy on the solid electrolyte interphase (SEI) layer of CS-C revealed a lesser amount of conductive SEI layer on its surface compared to MFC-C, mainly composed of NaF and an additional FSI-derived Na complex, suggested to be Na2 [SO3-N-SO2F]). In contrast, MFC-C revealed a greater amount of SEI-related compounds, which is interpreted as a thicker SEI layer resulting in a long Na+ diffusion pathway and slower Na+ reaction kinetics. This study provides insight into the effect of microstructural differences arising from different cellulose precursors on the electrochemical performance, thereby aiding in the fabrication and optimization of hard carbon anodes for sodium-ion batteries.
Evaluation of novel chromatographic prototypes for supercoiled plasmid DNA polishing
Authors: Ferreira Pedro L. , Marie Helena , Berger Tim , Edelmann Bianca , Rammo Oliver , Sousa Fani
Published: January 2024

Since the world first approved gene therapeutics, nucleic acid-based therapies have gained prominence. Several strategies for DNA-based therapy have been approved, and numerous clinical trials for plasmid DNA (pDNA)-based vaccines are currently in development. Due to the rising interest in pDNA for vaccination and gene therapy, plasmid manufacturing must become more effective. One of the most critical steps is downstream processing, involving isolation and purification procedures. To comply with the regulatory guidelines, pDNA must be available as a highly purified, homogeneous preparation of supercoiled pDNA (sc pDNA). This process undertakes several challenges, primarily due to the diversity of molecules derived from the producer organism. In this study, different resins were tested for the adsorption and selective polishing of sc pDNA. To identify optimal pDNA adsorption conditions, batch and column assays were performed with different resins while promoting electrostatic and hydrophobic interactions. The effect of ionic strength, pH, and contact time were evaluated and optimized. Additionally, static and dynamic binding capacities were determined for the selected resins. Analytical chromatography and agarose gel electrophoresis were used to assess the selectivity of the most promising resins toward sc pDNA isoform. Also, genomic DNA, endotoxins, and proteins were quantified to characterize the final sc pDNA quality. At the same time, the recovery and purity yields were evaluated by quantification of sc pDNA after the purification procedure. Overall, the results of the chromatographic assays using agmatine- and arginine-based resins have shown promising potential for sc pDNA polishing. Both resins demonstrated excellent binding capacity for pDNA, with agmatine outperforming arginine-based resin in terms of capacity. However, arginine-based resin exhibited a superior pDNA recovery yield, reaching a notable 52.2% recovery compared to 10.09% from agmatine. Furthermore, both resins exhibited high relative purity levels above 90% for the sc pDNA. The comprehensive characterization of the recovered sc pDNA also revealed a significant reduction in gDNA levels, reinforcing the potential of these prototypes for obtaining high-quality and pure sc pDNA. These findings highlight the promising applications of both resins in scalable pDNA purification processes for gene therapy and biopharmaceutical applications.
Injectable thermosensitive hydrogel loading erythropoietin and FK506 alleviates gingival inflammation and promotes periodontal tissue regeneration
Authors: Gu Zhongyi , Qiu Caiqing , Chen Ling , Wang Xiaoli
Published: January 2024

Background: Periodontitis is a chronic multifactorial inflammatory disease associated with dysbiotic plaque biofilms and characterized by progressive destruction of the tooth-supporting apparatus. Therefore, there is significant potential in the discovery of drugs that inhibit periodontal inflammatory responses and promote periodontal regeneration.

Methods: In this study, we generated a periodontitis rat model to detect the effects of chitosan/β-sodium glycerophosphate (β-GP)/glycolic acid (GA) hydrogel carried Erythropoietin and FK506 (EPO-FK506-CS/β-GP/GA). A total of forty-eight male Wistar rats were used to establish the periodontitis model. Drug injection was administered every 3 days for a total of five times over a 2-week period. After a period of 2 weeks following implantation, the rats underwent anesthesia, and a section of their maxillae encompassing the maxillary first and second molars, along with the alveolar bone, was obtained. micro-CT scanning, histopathology, immunohistochemistry and reverse transcription-quantitative PCR (RT-qPCR) assays were performed. Meanwhile, ELISA assay was performed to detect the levels of inflammatory mediators (TNF-α, IL-6 and IL-1β).

Results: The synthesis and characterization of EPO-FK506-CS/β-GP/GA revealed that the hydrogel has stability and sustained release of drugs. The application of FK506+EPO was found to significantly enhance new bone formation in the defect area, as evidenced by the results of HE staining. Additionally, the use of FK506+EPO in the treated groups led to a notable increase in the density of alveolar bone, as observed through micro-CT analysis, when compared to the Model group. EPO-FK506-CS/β-GP/GA hydrogel exhibited notable efficacy in modulating inflammatory mediators (TNF-α, IL-6 and IL-1β). Furthermore, the osteoinductive properties of the EPO-FK506-CS/β-GP/GA hydrogel were extensive, as evidenced by a significant upregulation in the expression of key markers (Collagen I, Runx2, OPN, and OCN) associated with osteoblastic differentiation.

Conclusion: Taken together, EPO-FK506-CS/β-GP/GA hydrogel alleviates gingival inflammation and promotes periodontal tissue regeneration in the periodontitis.
Comprehensive evaluation of plasma microbial cell-free DNA sequencing for predicting bloodstream and local infections in clinical practice: a multicenter retrospective study
Authors: Pang Feng , Xu Wenbin , Zhao Hui , Chen Shuai , Tian Yaxian , Fu Juanjuan , You Zhiqing , Song Pingping , Xian Qingjie , Zhao Qigang , Wang Chengtan , Jia Xiuqin
Published: January 2024

Background

Metagenomic next-generation sequencing (mNGS) of plasma cell-free DNA (cfDNA) shows promising application for complicated infections that cannot be resolved by conventional microbiological tests (CMTs). The criteria for cfDNA sequencing are currently in need of agreement and standardization.

Methods

We performed a retrospective cohort observation of 653 patients who underwent plasma cfDNA mNGS, including 431 with suspected bloodstream infections (BSI) and 222 with other suspected systemic infections. Plasma mNGS and CMTs were performed simultaneously in clinical practice. The diagnostic efficacy of plasma mNGS and CMTs in the diagnosis of blood-borne and other systemic infections was evaluated using receiver operating characteristic (ROC) curves. The sensitivity and specificity of the two methods were analyzed based on the final clinical outcome as the gold standard.

Results

The mNGS test showed an overall positive rate of 72.3% (472/653) for detecting microorganisms in plasma cfDNA, with a range of 2 to 6 different microorganisms detected in 171 patient specimens. Patients with positive mNGS results were more immunocompromised and had a higher incidence of severe disease (P<0·05). The sensitivity of mNGS was higher for BSI (93·5%) and other systemic infections (83·6%) compared to CMTs (37·7% and 14·3%, respectively). The mNGS detected DNA from a total of 735 microorganisms, with the number of microbial DNA reads ranging from 3 to 57,969, and a higher number of reads being associated with clinical infections (P<0·05). Of the 472 patients with positive mNGS results, clinical management was positively affected in 203 (43%) cases. Negative mNGS results led to a modified clinical management regimen in 92 patients (14.1%). The study also developed a bacterial and fungal library for plasma mNGS and obtained comparisons of turnaround times and detailed processing procedures for rare pathogens.

Conclusion

Our study evaluates the clinical use and analytic approaches of mNGS in predicting bloodstream and local infections in clinical practice. Our results suggest that mNGS has higher positive predictive values (PPVs) for BSI and systemic infections compared to CMTs, and can positively affect clinical management in a significant number of patients. The standardized whole-process management procedure for plasma mNGS developed in this study will ensure improved pre-screening probabilities and yield clinically valuable data.
Gut microbiota as predictors of the occurrence of high on-treatment platelet reactivity in acute ischemic stroke patients
Authors: Lou Zhenzhen , Ouyang Huiying , Chen Guixian , Li Xiaojun , Chen Haoxuan , Zhan Yibo , Peng Lilin , Du Chenghao , Zheng Zequan , Wen Longlong , Xu Haoyou , Zhao Min , Zhao Yuanqi
Published: January 2024

Introduction

In this study, we aimed to investigate the association between gut microbiota and high on-treatment platelet reactivity (HTPR) in patients with acute ischemic stroke (AIS).

Methods

We enrolled a total of 48 AIS patients, including 19 HTPR patients and 29 non-high on-treatment platelet reactivity (NHTPR) patients, along with 10 healthy controls. Clinical and laboratory data, as well as stool samples, were collected from all participants. The composition and function of gut microbiota were assessed using 16S rRNA sequencing. Differences in the gut microbiota between the two groups were analyzed, and a diagnostic model based on the gut microbiota was established using random forest model.

Results

HTPR patients exhibited a decreased microbial richness compared to NHTPR patients. Additionally, the relative abundance of unidentified_Clostridia and Ralstonia was lower in HTPR patients. Significant differences in biological functions, such as toxoplasmosis, were observed between the two groups. The combination of Ralstonia, unidentified-Clostridia, Mailhella, Anaerofustis, and Aggregatibacter showed excellent predictive ability for HTPR occurrence (AUC=0.896). When comparing AIS patients with healthy controls, alterations in the microbiota structure were observed in AIS patients, with imbalances in short-chain fatty acid-producing bacteria and pathogenic bacteria. Significant differences in biological functions, such as oxidative phosphorylation, were noted between the two groups. The combination of Alloprevotella, Terrisporobacter, Streptococcus, Proteus, and unidentified_Bacteria exhibited strong predictive power for AIS occurrence (AUC=0.994).

Conclusions

This study is the first to uncover the microbial characteristics of HTPR in AIS patients and demonstrate the predictive potential of specific bacterial combinations for HTPR occurrence.
Deciphering complex antibiotic resistance patterns in Helicobacter pylori through whole genome sequencing and machine learning
Authors: Yu Jianwei , Jia Yan , Yu Qichao , Lin Lan , Li Chao , Chen Bowang , Zhong Pingyu , Lin Xueqing , Li Huilan , Sun Yinping , Zhong Xuejing , He Yuqi , Huang Xiaoyun , Lin Shuangming , Pan Yuanming
Published: January 2024

Introduction

Helicobacter pylori (H.pylori, Hp) affects billions of people worldwide. However, the emerging resistance of Hp to antibiotics challenges the effectiveness of current treatments. Investigating the genotype-phenotype connection for Hp using next-generation sequencing could enhance our understanding of this resistance.

Methods

In this study, we analyzed 52 Hp strains collected from various hospitals. The susceptibility of these strains to five antibiotics was assessed using the agar dilution assay. Whole-genome sequencing was then performed to screen the antimicrobial resistance (AMR) genotypes of these Hp strains. To model the relationship between drug resistance and genotype, we employed univariate statistical tests, unsupervised machine learning, and supervised machine learning techniques, including the development of support vector machine models.

Results

Our models for predicting Amoxicillin resistance demonstrated 66% sensitivity and 100% specificity, while those for Clarithromycin resistance showed 100% sensitivity and 100% specificity. These results outperformed the known resistance sites for Amoxicillin (A1834G) and Clarithromycin (A2147), which had sensitivities of 22.2% and 87%, and specificities of 100% and 96%, respectively.

Discussion

Our study demonstrates that predictive modeling using supervised learning algorithms with feature selection can yield diagnostic models with higher predictive power compared to models relying on single single-nucleotide polymorphism (SNP) sites. This approach significantly contributes to enhancing the precision and effectiveness of antibiotic treatment strategies for Hp infections. The application of whole-genome sequencing for Hp presents a promising pathway for advancing personalized medicine in this context.
Macrophthalmus (Macrophthalmus) abbreviatus Manning & Holthuis, 1981, a new natural host for Hematodinium perezi infection
Authors: Liu Zhengmin , Xie Guosi , Wang Hailiang , Li Xinshu , Wan Xiaoyuan , Li Ang , Zhou Liqing , Shi Chengyin , Zhang Qingli , Huang Jie
Published: January 2024

Recent reports have shown that wild crabs may be important hosts involved in the transmission and spread of the parasitic Hematodinium in cultured marine crustaceans. Therefore, monitoring the prevalence of Hematodinium infections in wild crabs is necessary to develop effective strategies for the prevention and control of Hematodinium disease. Here we report a wild crab species, Macrophthalmus (Macrophthalmus) abbreviatus Manning & Holthuis, 1981, as a new natural host for Hematodinium sp. infection. It is one of the common wild crab species dwelling in the ponds or waterways connected to the polyculture ponds located on the coast of Rizhao or Weifang, Shandong Peninsula, China. According to the results of PCR detection and phylogenetic analysis targeting the internal transcribed spacer 1 (ITS 1) region, these Hematodinium sp. isolates were identified as H. perezi and fell into the genotype II category within H. perezi. A high monthly prevalence of H. perezi infection was observed during the 2021–2022 field survey, ranging from 33.3% to 90.6% in M. abbreviatus originating from Weifang (n=304 wild crabs) and from 53.6% to 92.9% in those from Rizhao (n=42 wild crabs). Artificial inoculation infection experiments demonstrated that M. abbreviatus could be infected by H. perezi, and massive Hematodinium cells and typical histopathological changes were observed in the hepatopancreas and gill tissues of the infected crabs. To our knowledge, this is the first report of M. abbreviatus as a new natural host for H. perezi infection. Results in the present study extend the known host spectrum for this emerging parasite pathogen, and also provide valuable information for epidemic surveillance of the Hematodinium disease as well.
Cognitive perspective of metaphors in Chinese Hua'er folksongs
Authors: Zhang Xiaojin
Published: January 2024

The cognitive frame and transferences from sources to targets of metaphor study have been evident in humanities. The present article aims to compare Chinese heritage Hua'er folksongs, Chinese new poetry, Chinese prose, Chinese news reportage, and Chinese news editorials. By adopting the indicator I1, the results show that the A = B metaphor is typically representative of Chinese Hua'er folksongs. At a more detailed level of comparison of the a-index, heritage folksong Hua'er bears the lowest level of metaphoricity for its repeated use of the metaphorical lexis both in the target domain and source domain. The genre-specific metaphorical expressions in Chinese poetic texts show that the higher a-index is, the more metaphoricity and more cognitive the effort will be. The nominalized metaphorical expressions in heritage Hua'er might limit the working memory constraints and the cognitive mastery of source-to-target structure for the singers to create metaphors.
Lived experience in human-building interaction (HBI): an initial framework
Authors: Economidou Eléni , Itzlinger Alina , Frauenberger Christopher
Published: January 2024

The emerging field of human-building interaction (HBI) has its roots in the historical trends of the development of architecture and human-computer interaction (HCI). Advancements in building information modelling (BIM), sensing, and actuation technologies as well as the commodification and miniaturisation of microprocessors over the past two decades are transforming what once were quixotic visions of a cybernetic architecture into reality. This new reality which integrates computation with architecture opens up different kinds of engagements in the ways we design, use, and inhabit our built environments. A question that follows this new reality is: how can we conceptualise human experience in such environments? Thus far, the lived human experience of such interactions has been an overlooked aspect in HBI-related research. In this article, we provide an initial experience framework for HBI underpinned by existing literature from the HCI and architecture domains on the subjective, lived-in experience of architecture and findings derived from a case study of a field-deployed HBI interface. The research objective of our framework is to outline aspects of HBI lived experiences that can be used as guiding lenses for HBI designers and practitioners who wish to design for and assess such experiences.
The impact of architectural form on physiological stress: a systematic review
Authors: Valentine Cleo
Published: January 2024

Technological advancements in physiological body sensor networks (i.e., biometric tracking wearables) and simulated environments (i.e., VR) have led to increased research in the field of neuroarchitecture, specifically investigating the effects of architectural forms, defined here as subtle variations in the shape or configuration of the interior built environment, on neurological responses. While this research field is still in its nascent stages, early findings suggest that certain architectural forms may impact physiological stress responses. Physiological stress has, in turn, been implicated in the development of certain diseases, including cardiovascular disease, cancer, chronic kidney disease, non-alcoholic fatty liver disease and autoimmune and neurodegenerative disorders. To aid future research, particularly into the relationship between media architecture and physiological stress, this paper conducts a systematic review following PRISMA-P guidelines on studies that evaluated physiological stress responses to architectural form using clinical biomarkers. The review identifies the specific clinical biomarkers used to evaluate physiological stress responses to architectural forms and the distinct categories of architectural forms that have, to date, been correlated with elevated stress responses: curvature, enclosure and proportion. Although these studies' findings imply that the identified architectural forms influence physiological stress, their generalisability is arguably constrained by several factors. These constraints include the paucity of research in this area, the lack of uniformity in the definition and measurement of these architectural forms, the varying contextual settings, the unisensory approach of research methodologies, and the duration of exposure under evaluation. The review concludes that clinical biomarkers may be used to measure the impact of architectural form on physiological stress; however, future research should strive for standardized approaches in defining and measuring architectural forms in order to increase the transferability and robustness of results.
Echoes of research and training with biographical narratives: ways of knowing and understanding from lived experience
Authors: Leal da Costa Conceição , Alves Camila Aloisio , Breton Hervé
Published: January 2024

This article aims to present the formative results obtained through the research-action-training device “Biographical workshop: learning about life and profession” (BW), proposed and implemented as a university extension activity in a higher education institution in Northeastern Brazil. Inspired by the epistemological and methodological bases of biographically oriented approaches in Education, the device was built with the intention of promoting, through biographical narrative, a reflective exercise both individual and collective focused on the exploration and reconstitution of personal and professional trajectories of the participants. The analyses produced from the narratives in its scope allow us to understand the different elements that give body and meaning to the trajectory of the participants in the course of their academic and professional training. Moreover, it was possible to apprehend the formative process inscribed in the mobilization of the narrative records, oral and written, and its effects for the participants. We conclude that BW is a powerful research-action-training device in that it articulates and values both the subject and the professional who is formed through lived experiences, integrating the spheres of life for an implicated, reflective and conscious professional action.
The multiple actions of grape and its polyphenols on female reproductive processes with an emphasis on cell signalling
Authors: Kohut Ladislav , Baldovska Simona , Mihal Michal , Belej Lubomir , Sirotkin Alexander V. , Roychoudhury Shubhadeep , Kolesarova Adriana
Published: January 2024

Grapes are an economically important fruit crop, and their polyphenols (mainly phenolic acids, flavanols, flavonols, anthocyanins, proanthocyanidins, and stilbenes) can exert a wide range of health benefits as an interesting and valuable dietary supplement for natural complementary therapy. However, their potential physiological and therapeutic actions on reproductive processes have not been sufficiently elucidated. This evidence-based study presents current knowledge of grape extracts and polyphenols, as well as their properties and therapeutical actions in relation to female reproduction in a nutshell. Grape extract, and its polyphenols such as resveratrol, proanthocyanidin B2 or delphinidin may influence female reproductive physiology and pathology, as well as regulate multiple signaling pathways related to reproductive hormones, steroid hormones receptors, intracellular regulators of oxidative stress and subsequent inflammation, apoptosis, and proliferation. Their role in the management of ovarian cancer, age-related reproductive insufficiency, ovarian ischemia, PCOS, or menopausal syndrome has been indicated. In particular, the potential involvement of grapeseed extracts and/or proanthocyanidin B2 and delphinidin on ovarian steroidogenesis, oocyte maturation, and developmental capacity has been implicated, albeit at different regulatory levels. Grape polyphenols exert a wide range of health benefits posing grape extract as an interesting and valuable dietary supplement for natural complementary therapy. This evidence-based study focuses on the actions of grapeseed extract and grape polyphenols on female reproductive processes at various regulatory levels and multiple signalling pathways by regulating reproductive hormones (GnRH, gonadotropins, prolactin, steroid hormones, IGFBP), steroid receptors, markers of proliferation and apoptosis. However, lack of knowledge of standardized dosages so far limits their clinical application despite the wide range of their biological and therapeutic potentials.
Transient gestational hypothyroxinemia accelerates and enhances ulcerative colitis-like disorder in the male offspring
Authors: Rivera Juan Carlos , Opazo Ma. Cecilia , Hernández-Armengol Rosario , Álvarez Oscar , Mendoza-León María José , Caamaño Esteban , Gatica Sebastian , Bohmwald Karen , Bueno Susan M. , González Pablo A. , Neunlist Michel , Boudin Helene , Kalergis Alexis M. , Riedel Claudia A.
Published: January 2024

Introduction

Gestational hypothyroxinemia (HTX) is a condition that occurs frequently at the beginning of pregnancy, and it correlates with cognitive impairment, autism, and attentional deficit in the offspring. Evidence in animal models suggests that gestational HTX can increase the susceptibility of the offspring to develop strong inflammation in immune-mediated inflammatory diseases. Ulcerative colitis (UC) is a frequent inflammatory bowel disease with unknown causes. Therefore, the intensity of ulcerative colitis-like disorder (UCLD) and the cellular and molecular factors involved in proinflammatory or anti-inflammatory responses were analyzed in the offspring gestated in HTX (HTX-offspring) and compared with the offspring gestated in euthyroidism (Control-offspring).

Methods

Gestational HTX was induced by the administration of 2-mercapto-1-methylimidazole in drinking water to pregnant mice during E10–E14. The HTX-offspring were induced with UCLD by the acute administration of dextran sodium sulfate (DSS). The score of UCLD symptomatology was registered every day, and colon histopathology, immune cells, and molecular factors involved in the inflammatory or anti-inflammatory response were analyzed on day 6 of DSS treatment.

Results

The HTX-offspring displayed earlier UCLD pathological symptoms compared with the Control-offspring. After 6 days of DSS treatment, the HTX-offspring almost doubled the score of the Control-offspring. The histopathological analyses of the colon samples showed signs of inflammation at the distal and medial colon for both the HTX-offspring and Control-offspring. However, significantly more inflammatory features were detected in the proximal colon of the HTX-offspring induced with UCLD compared with the Control-offspring induced with UCLD. Significantly reduced mRNA contents encoding for protective molecules like glutamate-cysteine ligase catalytic subunit (GCLC) and mucin-2 (MUC-2) were found in the colon of the HTX-offspring as compared with the Control-offspring. Higher percentages of Th17 lymphocytes were detected in the colon tissues of the HTX-offspring induced or not with UCLD as compared with the Control-offspring.

Discussion

Gestational HTX accelerates the onset and increases the intensity of UCLD in the offspring. The low expression of MUC-2 and GCLC together with high levels of Th17 Lymphocytes in the colon tissue suggests that the HTX-offspring has molecular and cellular features that favor inflammation and tissue damage. These results are important evidence to be aware of the impact of gestational HTX as a risk factor for UCLD development in offspring.