bluuebunny/crossref_metadata_2025 huggingface dataset:
Adjunctive Clotiapine for the Management of Delusions in Two Adolescents with Anorexia Nervosa
Authors: Pruccoli Jacopo , Leone Giulia Joy , Di Sarno Cristina , Vetri Luigi , Quatrosi Giuseppe , Roccella Michele , Parmeggiani Antonia
Published: December 2021

Clotiapine is an atypical antipsychotic indicated for the management of a series of acute psychotic disorders. The current literature lacks evidence concerning the tolerability and clinical use of this drug in the management of individuals with anorexia nervosa (AN). In this study, we report two cases of adolescents with AN, treated with clotiapine. The reason for the administration of clotiapine was, for both patients, the manifestation of bizarre delusions concerning food and calories. Patient 1 presented a presyncope after the first dose of clotiapine, and treatment was rapidly discontinued. Patient 2 was treated with clotiapine for 9 months; doses were titrated from 20 mg/day to 70 mg/day, with an improvement in the reported delusions, which also enhanced compliance with psychological and nutritional interventions. EKG, QTc, white blood count, and red blood count were not relevantly influenced by the introduction of clotiapine in either patient. No extrapyramidal effect was documented. These reports stress the need for further studies assessing the tolerability and potential effect of clotiapine in treating adolescents with AN and delusional symptomatology.
Individual Differences in Belief in Fake News about Election Fraud after the 2020 U.S. Election
Authors: Calvillo Dustin P. , Rutchick Abraham M. , Garcia Ryan J. B.
Published: December 2021

Fake news is a serious problem because it misinforms people about important issues. The present study examined belief in false headlines about election fraud after the 2020 U.S. presidential election. Belief in election fraud had dangerous consequences, including the deadly insurrection at the U.S. Capitol in January 2021. In the present study, participants rated the truthfulness of true and false headlines about the election, and then completed individual difference measures eight days after the election. Participants with more conservative ideology, greater presidential approval of the outgoing president, greater endorsement of general conspiracy narratives and poorer cognitive reflection demonstrated greater belief in false headlines about election fraud. Additionally, consuming more politically conservative election news was associated with greater belief in false headlines. Identifying the factors related to susceptibility to false claims of election fraud offers a path toward countering the influence of these claims by tailoring interventions aimed at decreasing belief in misinformation and decreasing conspiracy beliefs to those most susceptible.
Empowering Women in Finance through Developing Girls’ Financial Literacy Skills in the United States
Authors: Park Chong Myung , Kraus Aidan D. , Dai Yanling , Fantry Crystal , Block Turner , Kelder Betsy , Howard Kimberly A. S. , Solberg V. Scott H.
Published: December 2021

This study examines the effectiveness of a financial literacy program, Invest in Girls (IIG), in promoting financial capability among high school girls. Using a quasi-experimental separate-samples pretest-posttest design and a longitudinal qualitative study, the study aims to assess the program efficacy and investigate the perspectives of the female students on its impact on their knowledge, behavior, and future goals and aspirations. The results indicated that the participants had significantly higher confidence for engaging in financial literacy after the program. The findings from the longitudinal study also suggested that that the program was influencing the students in positive ways, increasing their financial capability and leading them to consider wide occupational pathways available in finance. Given the lack of female leaders in the world of finance, the IIG program aims to address gender disparity in financial knowledge and highlight the importance of building financial literacy skills among girls.
Novel Fuzzy-Based Optimization Approaches for the Prediction of Ultimate Axial Load of Circular Concrete-Filled Steel Tubes
Authors: Liao Jinsong , Asteris Panagiotis G. , Cavaleri Liborio , Mohammed Ahmed Salih , Lemonis Minas E. , Tsoukalas Markos Z. , Skentou Athanasia D. , Maraveas Chrysanthos , Koopialipoor Mohammadreza , Armaghani Danial Jahed
Published: December 2021

An accurate estimation of the axial compression capacity of the concrete-filled steel tubular (CFST) column is crucial for ensuring the safety of structures containing them and preventing related failures. In this article, two novel hybrid fuzzy systems (FS) were used to create a new framework for estimating the axial compression capacity of circular CCFST columns. In the hybrid models, differential evolution (DE) and firefly algorithm (FFA) techniques are employed in order to obtain the optimal membership functions of the base FS model. To train the models with the new hybrid techniques, i.e., FS-DE and FS-FFA, a substantial library of 410 experimental tests was compiled from openly available literature sources. The new model’s robustness and accuracy was assessed using a variety of statistical criteria both for model development and for model validation. The novel FS-FFA and FS-DE models were able to improve the prediction capacity of the base model by 9.68% and 6.58%, respectively. Furthermore, the proposed models exhibited considerably improved performance compared to existing design code methodologies. These models can be utilized for solving similar problems in structural engineering and concrete technology with an enhanced level of accuracy.
The Cap-Binding Complex CBC and the Eukaryotic Translation Factor eIF4E: Co-Conspirators in Cap-Dependent RNA Maturation and Translation
Authors: Mars Jean-Clement , Ghram Mehdi , Culjkovic-Kraljacic Biljana , Borden Katherine L. B.
Published: December 2021

The translation of RNA into protein is a dynamic process which is heavily regulated during normal cell physiology and can be dysregulated in human malignancies. Its dysregulation can impact selected groups of RNAs, modifying protein levels independently of transcription. Integral to their suitability for translation, RNAs undergo a series of maturation steps including the addition of the m7G cap on the 5′ end of RNAs, splicing, as well as cleavage and polyadenylation (CPA). Importantly, each of these steps can be coopted to modify the transcript signal. Factors that bind the m7G cap escort these RNAs through different steps of maturation and thus govern the physical nature of the final transcript product presented to the translation machinery. Here, we describe these steps and how the major m7G cap-binding factors in mammalian cells, the cap binding complex (CBC) and the eukaryotic translation initiation factor eIF4E, are positioned to chaperone transcripts through RNA maturation, nuclear export, and translation in a transcript-specific manner. To conceptualize a framework for the flow and integration of this genetic information, we discuss RNA maturation models and how these integrate with translation. Finally, we discuss how these processes can be coopted by cancer cells and means to target these in malignancy.
Integrin αE(CD103)β7 in Epithelial Cancer
Authors: Hoffmann Johanna C. , Schön Michael P.
Published: December 2021

Interactions of both the innate and the adaptive immune system with tumors are complex and often influence courses and therapeutic treatments in unanticipated ways. Based on the concept that CD8+T cells can mediate important antitumor effects, several therapies now aim to amplify their specific activity. A subpopulation of CD8+ tissue-resident T lymphocytes that express the αE(CD103)β7 integrin has raised particular interest. This receptor presumably contributes to the recruitment and retention of tumor-infiltrating immune cells through interaction with its ligand, E-cadherin. It appears to have regulatory functions and is thought to be a component of some immunological synapses. In TGF-rich environments, the αE(CD103)β7/E-cadherin-interaction enhances the binding strength between tumor cells and infiltrating T lymphocytes. This activity facilitates the release of lytic granule contents and cytokines as well as further immune responses and the killing of target cells. Expression of αE(CD103)β7 in some tumors is associated with a rather favorable prognosis, perhaps with the notable exception of squamous cell carcinoma of the skin. Although epithelial skin tumors are by far the most common tumors of fair-skinned people, there have been very few studies on the distribution of αE(CD103)β7 expressing cells in these neoplasms. Given this background, we describe here that αE(CD103)β7 is scarcely present in basal cell carcinomas, but much more abundant in squamous cell carcinomas with heterogeneous distribution. Notwithstanding a substantial number of studies, the role of αE(CD103)β7 in the tumor context is still far from clear. Here, we summarize the essential current knowledge on αE(CD103)β7 and outline that it is worthwhile to further explore this intriguing receptor with regard to the pathophysiology, therapy, and prognosis of solid tumors.
The Diverse Roles of γδ T Cells in Cancer: From Rapid Immunity to Aggressive Lymphoma
Authors: Schönefeldt Susann , Wais Tamara , Herling Marco , Mustjoki Satu , Bekiaris Vasileios , Moriggl Richard , Neubauer Heidi A.
Published: December 2021

γδ T cells are unique players in shaping immune responses, lying at the intersection between innate and adaptive immunity. Unlike conventional αβ T cells, γδ T cells largely populate non-lymphoid peripheral tissues, demonstrating tissue specificity, and they respond to ligands in an MHC-independent manner. γδ T cells display rapid activation and effector functions, with a capacity for cytotoxic anti-tumour responses and production of inflammatory cytokines such as IFN-γ or IL-17. Their rapid cytotoxic nature makes them attractive cells for use in anti-cancer immunotherapies. However, upon transformation, γδ T cells can give rise to highly aggressive lymphomas. These rare malignancies often display poor patient survival, and no curative therapies exist. In this review, we discuss the diverse roles of γδ T cells in immune surveillance and response, with a particular focus on cancer immunity. We summarise the intriguing dichotomy between pro- and anti-tumour functions of γδ T cells in solid and haematological cancers, highlighting the key subsets involved. Finally, we discuss potential drivers of γδ T-cell transformation, summarising the main γδ T-cell lymphoma/leukaemia entities, their clinical features, recent advances in mapping their molecular and genomic landscapes, current treatment strategies and potential future targeting options.
Senescence Alterations in Pulmonary Hypertension
Authors: Roger Inés , Milara Javier , Belhadj Nada , Cortijo Julio
Published: December 2021

Cellular senescence is the arrest of normal cell division and is commonly associated with aging. The interest in the role of cellular senescence in lung diseases derives from the observation of markers of senescence in chronic obstructive pulmonary disease (COPD), pulmonary fibrosis (IPF), and pulmonary hypertension (PH). Accumulation of senescent cells and the senescence-associated secretory phenotype in the lung of aged patients may lead to mild persistent inflammation, which results in tissue damage. Oxidative stress due to environmental exposures such as cigarette smoke also promotes cellular senescence, together with additional forms of cellular stress such as mitochondrial dysfunction and endoplasmic reticulum stress. Growing recent evidence indicate that senescent cell phenotypes are observed in pulmonary artery smooth muscle cells and endothelial cells of patients with PH, contributing to pulmonary artery remodeling and PH development. In this review, we analyze the role of different senescence cell phenotypes contributing to the pulmonary artery remodeling process in different PH clinical entities. Different molecular pathway activation and cellular functions derived from senescence activation will be analyzed and discussed as promising targets to develop future senotherapies as promising treatments to attenuate pulmonary artery remodeling in PH.
Unlocking the Complexity of Mitochondrial DNA: A Key to Understanding Neurodegenerative Disease Caused by Injury
Authors: Singh Larry N. , Kao Shih-Han , Wallace Douglas C.
Published: December 2021

Neurodegenerative disorders that are triggered by injury typically have variable and unpredictable outcomes due to the complex and multifactorial cascade of events following the injury and during recovery. Hence, several factors beyond the initial injury likely contribute to the disease progression and pathology, and among these are genetic factors. Genetics is a recognized factor in determining the outcome of common neurodegenerative diseases. The role of mitochondrial genetics and function in traditional neurodegenerative diseases, such as Alzheimer’s and Parkinson’s diseases, is well-established. Much less is known about mitochondrial genetics, however, regarding neurodegenerative diseases that result from injuries such as traumatic brain injury and ischaemic stroke. We discuss the potential role of mitochondrial DNA genetics in the progression and outcome of injury-related neurodegenerative diseases. We present a guide for understanding mitochondrial genetic variation, along with the nuances of quantifying mitochondrial DNA variation. Evidence supporting a role for mitochondrial DNA as a risk factor for neurodegenerative disease is also reviewed and examined. Further research into the impact of mitochondrial DNA on neurodegenerative disease resulting from injury will likely offer key insights into the genetic factors that determine the outcome of these diseases together with potential targets for treatment.
GLIS3: A Critical Transcription Factor in Islet β-Cell Generation
Authors: Scoville David W. , Jetten Anton M.
Published: December 2021

Understanding of pancreatic islet biology has greatly increased over the past few decades based in part on an increased understanding of the transcription factors that guide this process. One such transcription factor that has been increasingly tied to both β-cell development and the development of diabetes in humans is GLIS3. Genetic deletion of GLIS3 in mice and humans induces neonatal diabetes, while single nucleotide polymorphisms (SNPs) in GLIS3 have been associated with both Type 1 and Type 2 diabetes. As a significant progress has been made in understanding some of GLIS3’s roles in pancreas development and diabetes, we sought to compare current knowledge on GLIS3 within the pancreas to that of other islet enriched transcription factors. While GLIS3 appears to regulate similar genes and pathways to other transcription factors, its unique roles in β-cell development and maturation make it a key target for future studies and therapy.
Extracellular Signalling Modulates Scar/WAVE Complex Activity through Abi Phosphorylation
Authors: Singh Shashi Prakash , Thomason Peter A. , Insall Robert H.
Published: December 2021

The lamellipodia and pseudopodia of migrating cells are produced and maintained by the Scar/WAVE complex. Thus, actin-based cell migration is largely controlled through regulation of Scar/WAVE. Here, we report that the Abi subunit—but not Scar—is phosphorylated in response to extracellular signalling in Dictyostelium cells. Like Scar, Abi is phosphorylated after the complex has been activated, implying that Abi phosphorylation modulates pseudopodia, rather than causing new ones to be made. Consistent with this, Scar complex mutants that cannot bind Rac are also not phosphorylated. Several environmental cues also affect Abi phosphorylation—cell-substrate adhesion promotes it and increased extracellular osmolarity diminishes it. Both unphosphorylatable and phosphomimetic Abi efficiently rescue the chemotaxis of Abi KO cells and pseudopodia formation, confirming that Abi phosphorylation is not required for activation or inactivation of the Scar/WAVE complex. However, pseudopodia and Scar patches in the cells with unphosphorylatable Abi protrude for longer, altering pseudopod dynamics and cell speed. Dictyostelium, in which Scar and Abi are both unphosphorylatable, can still form pseudopods, but migrate substantially faster. We conclude that extracellular signals and environmental responses modulate cell migration by tuning the behaviour of the Scar/WAVE complex after it has been activated.
Orai3 Calcium Channel Regulates Breast Cancer Cell Migration through Calcium-Dependent and -Independent Mechanisms
Authors: Chamlali Mohamed , Kouba Sana , Rodat-Despoix Lise , Todesca Luca Matteo , Pethö Zoltán , Schwab Albrecht , Ouadid-Ahidouch Halima
Published: December 2021

Orai3 calcium (Ca2+) channels are implicated in multiple breast cancer processes, such as proliferation and survival as well as resistance to chemotherapy. However, their involvement in the breast cancer cell migration processes remains vague. In the present study, we exploited MDA-MB-231 and MDA-MB-231 BrM2 basal-like estrogen receptor-negative (ER−) cell lines to assess the direct role of Orai3 in cell migration. We showed that Orai3 regulates MDA-MB-231 and MDA-MB-231 BrM2 cell migration in two distinct ways. First, we showed that Orai3 remodels cell adhesive capacities by modulating the intracellular Ca2+ concentration. Orai3 silencing (siOrai3) decreased calpain activity, cell adhesion and migration in a Ca2+-dependent manner. In addition, Orai3 interacts with focal adhesion kinase (FAK) and regulates the actin cytoskeleton, in a Ca2+-independent way. Thus, siOrai3 modulates cell morphology by altering F-actin polymerization via a loss of interaction between Orai3 and FAK. To summarize, we demonstrated that Orai3 regulates cell migration through a Ca2+-dependent modulation of calpain activity and, in a Ca2+-independent manner, the actin cytoskeleton architecture via FAK.
Zinner Syndrome—A Rare Cause of Recurrent Epididymitis and Infertility
Authors: Ostrowska Magdalena , Grześk Magdalena , Kaczyński Szymon , Skwara Dominika , Kulik Kacper , Kowalski Filip , Ostrowski Adam , Drewa Tomasz
Published: December 2021

Zinner syndrome (ZS) is a rare disorder that affects only men. It is characterized by a triad of abnormalities, including unilateral renal agenesis, ipsilateral seminal vesicle cysts, and atresia of the ejaculatory tract. Unfortunately, there is no consensus on the best treatment modality. We describe a case of a young male patient with recurrent epididymitis, dysuria, and frequent urination. In the diagnostic evaluation, we found an extended right seminal vesicle in the ultrasound with hyperechoic fluid inside and an absence of the right kidney. We performed magnetic resonance imaging, computed tomography, and semen analysis confirming Zinner syndrome and deteriorated semen parameters. Urethroscopic evaluation and ultrasound-guided puncture of the seminal vesicle were performed. An abscess was excluded. The cytologic evaluation showed hemosiderophages. Tamsulosin was introduced. We found no signs of relapse in a six-month observation, and the patient had no further symptoms. Therefore, minimally invasive treatment is a feasible option in young patients found with early-stage Zinner syndrome.
Pulsed Laser Deposition and Laser-Induced Backward Transfer to Modify Polydimethylsiloxane
Authors: Cutroneo Mariapompea , Havranek Vladimir , Flaks Josef , Malinsky Petr , Torrisi Lorenzo , Silipigni Letteria , Slepicka Petr , Fajstavr Dominik , Mackova Anna
Published: December 2021

Polydimethylsiloxane (PDMS) is a silicone-elastomer that owes its large application in the field of stretchable electronics to its chemical and thermal stability, transparency, flexibility, non-toxicity, compatibility, and low cost. PDMS is a versatile material because it can be used both as an elastic substrate and, after functionalization, as an active material for the design of stretchable electronics. One possible route for the functionalization of PDMS, thus becoming an active material together with numerous metals and semiconductors, is the embedding of conductive nanomaterials. Presently, pulsed laser deposition (PLD) and laser-induced backward transfer (LIBT) are used to deposit carbon- based material on polydimethylsiloxane. In this study, we explore and compare the surface treatments, advantages, and disadvantages of both different employed techniques in different environments. The modification of the wettability, elasticity, morphology, composition, and optical characteristics of polydimethylsiloxane will be evaluated by surface techniques such as scanning electron microscopy, Rutherford backscattering spectrometry, and the sessile drop method.
Preparation and Characterization of NiCr/NiCr-Al2O3/Al2O3 Multilayer Gradient Coatings by Gas Detonation Spraying
Authors: Rakhadilov Bauyrzhan , Buitkenov Dastan , Sagdoldina Zhuldyz , Idrisheva Zhanat , Zhamanbayeva Manira , Kakimzhanov Dauir
Published: December 2021

This paper investigates the influence of the technological parameters of detonation spraying on the phase composition of NiCr- and Al2O3-based coatings. It was determined that the phase composition of Al2O3 coatings during detonation spraying strongly depends on the barrel filling volume with the gas mixture. The acetylene–oxygen mixture, which is the most frequently used fuel in the detonation spraying of powder materials, was used as a fuel gas. To obtain a ceramic layer based on Al2O3, spraying was performed at an acetylene–oxygen O2/C2H2 mixture ratio of 1.856; the volume of filling of the detonation gun barrel with an explosive gas mixture was 63%. To obtain a NiCr-based metallic layer, spraying was performed at the O2/C2H2 ratio of 1.063; the volume of filling of the detonation gun barrel with an explosive gas mixture was 54%. Based on a study of the effect of the detonation spraying mode on the phase composition of NiCr and Al2O3 coatings, NiCr/NiCr-Al2O3/Al2O3-based multilayer coatings were obtained. Mixtures of NiCr/Al2O3 powders with different component ratios were used to obtain multilayer gradient coatings. The structural-phase composition, mechanical and tribological properties of multilayer gradient metal–ceramic coatings in which the content of the ceramic phase changes smoothly along the depth were experimentally investigated. Three-, five- and six-layer gradient coatings were obtained by alternating metallic (NiCr) and ceramic (Al2O3) layers. The phase composition of all coatings was found to correspond to the removal of information from a depth of 20–30 μm. It was determined that the five-layer gradient coating, consisting of the lower metal layer (NiCr), the upper ceramic layer (Al2O3) and the transition layer of the mechanical mixture of metal and ceramics, is characterized by significantly higher hardness (15.9 GPa), wear resistance and adhesion strength.
Effect of Corundum and Basalt Aggregates on the Ballistic Resistance of UHP-SFRC
Authors: Mára Michal , Kheml Přemysl , Carrera Kristýna , Fornůsek Jindřich , Sovják Radoslav
Published: December 2021

Ultra-high-performance steel-fibre-reinforced concrete (UHP-SFRC) is a technologically advanced composite with a high ability to absorb and dissipate mechanical energy. This work investigates the possibility of increasing ballistic resistance by adding different percentages of corundum and basalt aggregate into this type of concrete. The most common type of ammunition, a 7.62 mm × 39 mm calibre with a full-metal jacket and a mild-steel core (FMJ-MSC), was used to test all samples. The size of the damage and the mode of failure were determined using a 3D scanner operating on the principle of photogrammetry. The experimental campaign showed that the addition of basalt and, especially, corundum aggregate has a positive effect on ballistic resistance. In particular, the increase in compressive strength and the slight decrease in depth of penetration (DOP) was observed in the case of the usage of the corundum aggregate.
The Effects of Benzene on the Structure and Properties of Triethylamine Hydrochloride/Chloroaluminate
Authors: Tian Guocai , Du Huanhuan , Yuan Qingxiang
Published: December 2021

The effects of benzene (C6H6) on the radial distribution function, coordination number, spatial distribution function, physical and chemical properties such as density, viscosity, conductivity and transport properties of triethylamine hydrochloride /chloroaluminate ([Et3NH] Cl/AlCl3) ionic liquid were studied by first principle and molecular dynamics simulation. The stable geometry and electronic properties of benzene and ionic liquids, as well as their optimized adsorption on Cu (111) surface were obtained. The density, viscosity and conductivity obtained agreed well with the experimental values. It is found that the adsorption of cations, anions and benzene on Cu (111) surface is physical adsorption, and the adsorption capacity is [Et3NH] > C6H6 > Al2Cl7−. With the increase of benzene concentration, the density of the system decreases gradually, the interaction between cations and anions gradually weakens, resulting in the decrease of viscosity, the enhancement of diffusion and the increase of conductivity. Since the diffusion and adsorption capacity of benzene are greater than that of electroactive ion of Al2Cl7−, benzene would be easier to adsorb on the protruding part of the electrode surface, so as to reduce the effective surface area of the cathode, slow down the reduction speed of Al2Cl7− on the cathode surface and increase the over-potential, so the grain refined deposition layers can be obtained in electrodeposition.
Detection of Reproductive Hormones in Females by Using 1D Photonic Crystal-Based Simple Reconfigurable Biosensing Design
Authors: Aly Arafa , Awasthi S. , Mohamed A. , Matar Z. , Mohaseb M. , Al-Dossari M. , Tammam M. , Zaky Zaky , Amin A. , Sabra Walied
Published: December 2021

In this manuscript, we have explored the photonic biosensing application of the 1D photonic crystal (PhC) (AB)NCDC(AB)N, which is capable of detecting reproductive progesterone and estradiol hormones of different concentration levels in blood samples of females. The proposed structure is composed of an air cavity surrounded by two buffer layers of material MgF2, which is sandwiched between two identical 1D sub PhCs (AB)N. Both sub PhCs are made up of alternate layers of materials, SiO2 and Si, of period 5. MATLAB software has been used to obtain transmission characteristics of the structure corresponding TE wave, only with the help of the transfer matrix method. The mainstay of this research is focused on the dependence of the intensity and position of the defect mode inside the photonic bandgap with respect to reproductive hormone concentrations in blood samples, change in the thickness of the cavity region and change in angle of incidence corresponding to TE wave only. The proposed design shows high sensitivity of 98.92 nm/nmol/L and 96.58 nm/nmol/L when the cavity of a thickness of 340 nm is loaded with progesterone and estradiol hormones of concentrations of 80 nmol/L and 11 nmol/L, respectively, at an incident angle of 20°. Apart from sensitivity, other parameters such as quality factor and figure of merit have also been computed to gain deep insight about the sensing capabilities of the proposed design. These findings may pave the path for the design and development of various sensing devices capable of detecting gynecological problems pertaining to reproductive hormones in females. Thus, the simple design and excellent performance makes our design most efficient and suitable for sensing applications in industrial and biomedical fields.
Stabilization of Long-Pitch Supertwisted Nematic Structures
Authors: Ito Masahiro , Ohmi Satoshi , Takatoh Kohki
Published: December 2021

Stabilized reverse twisted nematic liquid crystal devices (RTN-LCDs) were fabricated using formation of a polymer matrix under UV irradiation with an applied voltage (sustain voltage) in the vicinity of the alignment layers. In the absence of an applied voltage, the non-stabilized RTN structure gradually returns to a splay twist structure. The sustain voltage was decreased with an increase in temperature. A stabilized long-pitch supertwisted nematic (LPSTN) structure could also be formed during the RTN structure stabilization process with a much lower sustain voltage at a temperature near the clearing point. The chiral pitch for the LPSTN structure is longer than that for a typical STN structure. LPSTN-LCDs similar to RTN-LCDs show a large reduction in both the threshold and saturation voltage compared with those for TN-LCDs consisted of the same LC materials. Furthermore, a notable feature of LPSTN-LCDs is a change to a TN structure when a high voltage is applied. A black state can be realized due to the change from the LPSTN structure to the RTN structure unlike the typical STN mode under the crossed nicols condition. In contrast STN-LCDs retain their color due to the retardation because the RTN and LPSTN states are considered topologically equivalent.
The Challenge to the Pathologist of PD-L1 Expression in Tumor Cells of Non-Small-Cell Lung Cancer—An Overview
Authors: Jöhrens Korinna , Rüschoff Josef
Published: December 2021

In recent years, the treatment of non-small-cell lung cancer (NSCLC) has been fundamentally changed by immunotherapy where the immune system is reactivated using anti-programmed cell death protein 1/programmed death ligand 1 (PD1/PD-L1) checkpoint inhibition. With this, the immunohistological detection of PD-L1 has become one of the most important predictive biomarkers, leading pathologists to play a central role in the immuno-oncological therapy decisions. This has brought them the challenge of requiring the knowledge of relevant checkpoint inhibitors (CI), different PD-L1 scores and cut-offs as well as the choice of the right tissues and controls. Their involvement is also required in the careful validation of both clinical trial assays (CTAs) and laboratory developed tests (LDTs), in addition to the internal and external quality assessment and the interpretation and scoring of the staining based on specialist training. After the training of tumor proportion score (TPS) scoring in NSCLC, pathologists show a high level of concordance, with some variation between different cut-offs. Since not all patients benefit from immunotherapy, further research is needed to validate new predictive markers and optimize existing ones. In this context, these studies focus on a combination of PD-L1 and molecular signatures.
Role of Optical Coherence Tomography Imaging in Predicting Progression of Age-Related Macular Disease: A Survey
Authors: Elsharkawy Mohamed , Elrazzaz Mostafa , Ghazal Mohammed , Alhalabi Marah , Soliman Ahmed , Mahmoud Ali , El-Daydamony Eman , Atwan Ahmed , Thanos Aristomenis , Sandhu Harpal Singh , Giridharan Guruprasad , El-Baz Ayman
Published: December 2021

In developed countries, age-related macular degeneration (AMD), a retinal disease, is the main cause of vision loss in the elderly. Optical Coherence Tomography (OCT) is currently the gold standard for assessing individuals for initial AMD diagnosis. In this paper, we look at how OCT imaging can be used to diagnose AMD. Our main aim is to examine and compare automated computer-aided diagnostic (CAD) systems for diagnosing and grading of AMD. We provide a brief summary, outlining the main aspects of performance assessment and providing a basis for current research in AMD diagnosis. As a result, the only viable alternative is to prevent AMD and stop both this devastating eye condition and unwanted visual impairment. On the other hand, the grading of AMD is very important in order to detect early AMD and prevent patients from reaching advanced AMD disease. In light of this, we explore the remaining issues with automated systems for AMD detection based on OCT imaging, as well as potential directions for diagnosis and monitoring systems based on OCT imaging and telemedicine applications.
Pemafibrate Ameliorates Liver Dysfunction and Fatty Liver in Patients with Non-Alcoholic Fatty Liver Disease with Hypertriglyceridemia: A Retrospective Study with the Outcome after a Mid-Term Follow-Up
Authors: Ikeda Suguru , Sugihara Takaaki , Kihara Takuya , Matsuki Yukako , Nagahara Takakazu , Takata Tomoaki , Kitao Sonoko , Okura Tsuyoshi , Yamamoto Kazuhiro , Isomoto Hajime
Published: December 2021

Non-alcoholic fatty liver disease (NAFLD) is a chronic liver disease related to metabolic syndrome. No standard pharmacological treatment has yet been established. We retrospectively evaluated the efficacy of pemafibrate in 16 NAFLD patients (11 men and 5 women; median age, 59 years; range, 27–81 years) who had taken pemafibrate for at least one year. They were all diagnosed with fatty liver according to imaging and clinical criteria. They were administered pemafibrate from October 2018 to October 2021 (median, 94 weeks; range, 56–157 weeks). Serum triglyceride was significantly decreased by −41.9% (342.3 ± 54.0 to 198.9 ± 20.4 mg/dL, p < 0.001). Aspartate aminotransferase (AST), alanine aminotransferase, and gamma-glutamyl transferase levels significantly decreased by −42.1% (49.6 ± 7.0 to 28.7 ± 3.4 U/L, p < 0.001), −57.1% (65.1 ± 10.8 to 27.9 ± 3.7 U/L, p < 0.001), and −43.2% (68.9 ± 10.9 to 39.1 ± 5.3 U/L, p < 0.05), respectively. The AST to platelet ratio (APRI) (0.8 ± 0.1 to 0.4 ± 0.1, p < 0.001) and fibrosis based on four factors (FIB-4) index (1.8 ± 0.3 to 1.4 ± 0.2, p < 0.05) also significantly decreased. Liver attenuation (39.1 ± 1.2 to 57.8 ± 2.7 HU, p = 0.028) and liver/spleen ratio (0.76 ± 0.04 to 1.18 ± 0.02, p = 0.012) significantly improved in three patients, as assessed by computed tomography. In conclusion, pemafibrate significantly improves serum triglyceride levels, liver function, FIB-4 index, APRI, and fatty liver in NAFLD patients with hypertriglyceridemia.
Artificial Intelligence and OCT Angiography in Full Thickness Macular Hole. New Developments for Personalized Medicine
Authors: Rizzo Stanislao , Savastano Alfonso , Lenkowicz Jacopo , Savastano Maria , Boldrini Luca , Bacherini Daniela , Falsini Benedetto , Valentini Vincenzo
Published: December 2021

Purpose: To evaluate the 1-year visual acuity predictive performance of an artificial intelligence (AI) based model applied to optical coherence tomography angiography (OCT-A) vascular layers scans from eyes with a full-thickness macular hole (FTMH). Methods: In this observational cross-sectional, single-center study, 35 eyes of 35 patients with FTMH were analyzed by OCT-A before and 1-year after surgery. Superficial vascular plexus (SVP) and deep vascular plexus (DVP) images were collected for the analysis. AI approach based on convolutional neural networks (CNN) was used to generate a continuous predictive variable based on both SVP and DPV. Different pre-trained CNN networks were used for feature extraction and compared for predictive accuracy. Results: Among the different tested models, the inception V3 network, applied on the combination of deep and superficial OCT-A images, showed the most significant differences between the two obtained image clusters defined in C1 and C2 (best-corrected visual acuity (BCVA) C1 = 66.67 (16.00 SD) and BCVA C2 = 49.10 (18.60 SD, p = 0.005). Conclusions: The AI-based analysis of preoperative OCT-A images of eyes affected by FTMH may be a useful support system in setting up visual acuity recovery prediction. The combination of preoperative SVP and DVP images showed a significant morphological predictive performance for visual acuity recovery.
The Translational Role of MUC8 in Salivary Glands: A Potential Biomarker for Salivary Stone Disease?
Authors: Schicht Martin , Reichle Adrian , Schapher Mirco , Garreis Fabian , Kleinsasser Benedikt , Aydin Malik , Sahin Afsun , Iro Heinrich , Paulsen Friedrich
Published: December 2021

Mucin (MUC) 8 has been shown to play an important role in respiratory disease and inflammatory responses. In the present study, we investigated the question of whether MUC8 is also produced and secreted by salivary glands and whether it may also play a role in the oral cavity in the context of inflammatory processes or in the context of salivary stone formation. Tissue samples from parotid and submandibular glands of body donors (n = 6, age range 63–88 years), as well as surgically removed salivary stones from patients (n = 38, age range 48–72 years) with parotid and submandibular stone disease were immunohistochemically analyzed targeting MUC8 and TNFα. The presence of MUC8 in salivary stones was additionally analyzed by dot blot analyses. Moreover, saliva samples from patients (n = 10, age range 51–72 years), who had a salivary stone of the submandibular gland on one side were compared with saliva samples from the other “healthy” side, which did not have a salivary stone, by ELISA. Positive MUC8 was detectable in the inter- and intralobular excretory ducts of both glands (parotid and submandibular). The glandular acini showed no reactivity. TNFα revealed comparable reactivity to MUC8 in the glandular excretory ducts and also did not react in glandular acini. Salivary stones demonstrated a characteristic distribution pattern of MUC8 that differed between parotid and submandibular salivary stones. The mean MUC8 concentration was 71.06 ng/mL in female and 33.21 ng/mL in male subjects (p = 0.156). Saliva from the side with salivary calculi contained significantly (15-fold) higher MUC8 concentration levels than saliva from the healthy side (p = 0.0005). MUC8 concentration in salivary stones varied from 4.59 ng/mL to 202.83 ng/mL. In females, the MUC8 concentration in salivary stones was significantly (2.3-fold) higher, with an average of 82.84 ng/mL compared to 25.27 ng/mL in male patients (p = 0.034). MUC8 is secreted in the excretory duct system of salivary glands and released into saliva. Importantly, MUC8 salivary concentrations vary greatly between individuals. In addition, the MUC8 concentration is gender-dependent (♀ > ♂). In the context of salivary stone diseases, MUC8 is highly secreted in saliva. The findings support a role for MUC8 in the context of inflammatory events and salivary stone formation. The findings allow conclusions on a gender-dependent component of MUC8.
Entropy in Image Analysis III
Authors: Sparavigna Amelia Carolina
Published: December 2021

Image analysis basically refers to any extraction of information from images, which can be as simple as QR codes required in logistics and digital certifications or related to large and complex datasets, such as the collections of images used for biometric identification or the sets of satellite surveys employed in the monitoring of Earth’s climate changes [...]
Performance Evaluation of a Maisotsenko Cycle Cooling Tower with Uneven Length of Dry and Wet Channels in Hot and Humid Conditions
Authors: Fan Xuchen , Lu Xiaofeng , Wang Jiping , Li Zilong , Wang Quanhai , Dong Zhonghao , Zhang Rongdi
Published: December 2021

The use of the Maisotsenko cycle (M-Cycle) in traditional wet cooling towers (TWCTs) has the potential to reduce the costs of electricity generation by cooling water below the inlet air’s wet-bulb temperature. TWCTs cannot provide sufficient cooling capacity for the increasing demand for cooling energy in the power and industrial sectors—especially in hot and wet climates. Due to this fact, an experimental system of an M-Cycle cooling tower (MCT) with parallel counter-flow arrangement fills was constructed in order to provide perspective on the optimal length of dry channels (ldry), thermal performance under different conditions, and pressure drops of the MCT. Results showed that the optimal value of ldry was 2.4 m, and the maximum wet-bulb effectiveness was up to 180%. In addition, the impact of air velocity in wet channels on the pressure drops of the novel fills was also summarized. This study confirms the great potential of using the M-Cycle in TWCTs, and provides a guideline for the industrial application and performance improvement of MCTs.
Techno-Economic Assessment of Biological Biogas Upgrading Based on Danish Biogas Plants
Authors: Lawson Néméhie , Alvarado-Morales Merlin , Tsapekos Panagiotis , Angelidaki Irini
Published: December 2021

Biological biogas upgrading with H2 derived from excess renewable electricity was modeled and simulated in PROII® (AVEVA Group plc, Cambridge, UK). An economic analysis was performed for a biogas plant processing 100,000 tons of biomass (substrate) per year. The biogas and biomethane production simulation results were validated with laboratory experimental data, as well as full-scale data obtained from biogas plants. A biomethane production cost of 0.47 €/Nm3 was calculated, while the minimum biomethane selling price for NPV = 0 was equal to 0.66 €/Nm3, considering a H2 price of 1.0 €/kg. The feasibility analysis indicated that the H2-related costs were the major contributor to the capital and operation costs due to high expenses associated with the in-situ H2 storage facility and the purchasing of H2, respectively. Compared to conventional upgrading methods, biological biogas upgrading has a higher capital and production cost, which can be reduced by increasing the plant capacity. The sensitivity analysis showed that the profitability is very sensitive to biomethane prices, capital investment, and the H2 price.
Recent Progress and Approaches on Transition Metal Chalcogenides for Hydrogen Production
Authors: Shanmugaratnam Sivagowri , Yogenthiran Elilan , Koodali Ranjit , Ravirajan Punniamoorthy , Velauthapillai Dhayalan , Shivatharsiny Yohi
Published: December 2021

Development of efficient and affordable photocatalysts is of great significance for energy production and environmental sustainability. Transition metal chalcogenides (TMCs) with particle sizes in the 1–100 nm have been used for various applications such as photocatalysis, photovoltaic, and energy storage due to their quantum confinement effect, optoelectronic behavior, and their stability. In particular, TMCs and their heterostructures have great potential as an emerging inexpensive and sustainable alternative to metal-based catalysts for hydrogen evolution. Herein, the methods used for the fabrication of TMCs, characterization techniques employed, and the different methods of solar hydrogen production by using different TMCs as photocatalyst are reviewed. This review provides a summary of TMC photocatalysts for hydrogen production.
Application of Artificial Neural Networks in the Urban Building Energy Modelling of Polish Residential Building Stock
Authors: Zygmunt Marcin , Gawin Dariusz
Published: December 2021

The development of energy-efficient buildings and sustainable energy supply systems is an obligatory undertaking towards a more sustainable future. To protect the natural environment, the modernization of urban infrastructure is indisputably important, possible to achieve considering numerous buildings as a group, i.e., Building Energy Cluster (BEC). The urban planning process evaluates multiple complex criteria to select the most profitable scenario in terms of energy consumption, environmental protection, or financial profitability. Thus, Urban Building Energy Modelling (UBEM) is presently a popular approach applied for studies towards the development of sustainable cities. Today’s UBEM tools use various calculation methods and approaches, as well as include different assumptions and limitations. While there are several popular and valuable software for UBEM, there is still no such tool for analyses of the Polish residential stock. In this work an overview on the home-developed tool called TEAC, focusing on its’ mathematical model and use of Artificial Neural Networks (ANN). An exemplary application of the TEAC software is also presented.
Anaerobic Digestion of Cigarette Butts: Microbial Community Analysis and Energy Production Estimation
Authors: Choi Okkyoung , Hwang Sae Eun , Park Hyojung , Sang Byoung-In
Published: December 2021

Anaerobic digestion using cigarette butts, one of most littered items, was studied not only as a waste treatment, but also as an energy production method. Methane production from cigarette butts was measured through the biochemical methane potential (BMP) test and it was evaluated whether it is possible to produce electrical energy. Intact cigarettes or individual components (filter, paper, and leaf) were supplied as the sole carbon source (substrate) for the BMP test. The tendency of methane production indicated biodegradation in the order of paper, filter, and leaves; however, the filter of cigarettes was the substrate produced the highest amount of methane per total solid. The microbial community was also analyzed in each anaerobic digestion reactor, and substrate-specific microorganisms were identified, such as Proteiniphilum strain (filter) and Methanobacterium formicicum (paper). In intact cigarettes, the related microbial community became dominant over time in the order of paper, filter, and leaf. The conversion of cigarette butts to methane, a renewable energy source, can be proposed as a sustainable route for energy demand, for example, in a smoking room.
Increasing the Biogas Potential of Rapeseed Straw Using Pulsed Electric Field Pre-Treatment
Authors: Szwarc Dawid , Głowacka Katarzyna
Published: December 2021

Due to the high availability of lignocellulosic biomass, which can be obtained from terrestrial plants, agricultural waste biomass, and the agro-food, paper or wood industries, its use for energy production by methane fermentation is economically and environmentally justified. However, due to their complex structures, lignocellulosic substrates have a low conversion factor to biogas. Therefore, scientists are still working on the development of new methods of the pre-treatment of lignocellulosic materials that will increase the biogas productivity from lignocellulosic biomass. The presented research focuses on the use of a pulsed electric field (PEF) to disintegrate rapeseed straw prior to the methane fermentation process. Scanning electron microscopy observation showed that, in the disintegrated sample, the extent of damage to the plant tissue was more severe than in the control sample. In the sample disintegrated for 7 min, the chemical oxygen demand increased from 4146 ± 75 mg/L to 4920 ± 60 mg/L. The best result was achieved with a 5-min PEF pre-treatment. The methane production reached 290.8 ± 12.1 NmL CH4/g VS, and the biogas production was 478.0 ± 27.5 NmL/g VS; it was 14% and 15% higher, respectively, compared to the control sample.
Bidding Agents for PV and Electric Vehicle-Owning Users in the Electricity P2P Trading Market
Authors: Sagawa Daishi , Tanaka Kenji , Ishida Fumiaki , Saito Hideya , Takenaga Naoya , Nakamura Seigo , Aoki Nobuaki , Nameki Misuzu , Saegusa Kosuke
Published: December 2021

As the world strives to decarbonize, the effective use of renewable energy has become an important issue, and P2P power trading is expected to unlock the value of renewable energy and encourage its adoption by enabling power trading based on user needs and user assets. In this study, we constructed a bidding agent that optimizes bids based on electricity demand and generation forecasts, user preferences for renewable energy (renewable energy-oriented or economically oriented), and owned assets in a P2P electricity trading market, and automatically performs electricity trading. The agent algorithm was used to evaluate the differences in trading content between different asset holdings and preferences by performing power sharing in a real scale environment. The demonstration experiments show that: EV-owning and economy-oriented users can trade more favorably in the market with a lower average execution price than non-EV-owning users; forecasting enables economy-enhancing moves to store nighttime electricity in batteries in advance in anticipation of future power generation and market prices; EV-owning and renewable energy-oriented users can trade more favorably in the market with other users. EV-owning and renewable energy-oriented users can achieve higher RE ratios at a cost of about +1 yen/kWh compared to other users. By actually issuing charging and discharging commands to the EV and controlling the charging and discharging, the agent can control the actual use of electricity according to the user’s preferences.