bluuebunny/crossref_metadata_2025 huggingface dataset:
Thermodynamics, Kinetics and Dilational Visco-Elasticity of Adsorbed CnEOm Layers at the Aqueous Solution/Air Interface
Authors: Fainerman Valentin B. , Kovalchuk Volodymyr I. , Aksenenko Eugene V. , Ravera Francesca , Liggieri Libero , Loglio Giuseppe , Makievski Alexander V. , Schneck Emanuel , Miller Reinhard
Published: March 2021

The adsorption behaviour of linear poly(oxyethylene) alkyl ether (CnEOm) is best described by a reorientation model. Based on a complete set of experimental data, including the adsorption kinetics, the equilibrium surface tension isotherm and the surface dilational visco-elasticity, the thermodynamic and kinetic adsorption parameters for some CnEOm at the water/air interface were determined. For the study, six CnEOm surfactants were selected (n = 10, 12 and 14 and m = 4, 5 and 8) and were studied by bubble profile analysis and maximum bubble pressure tensiometry. A refined theoretical model based on a reorientation-adsorption model combined with a diffusion-controlled adsorption kinetics and exchange of matter allowed us to calculate the surface layer composition by adsorbing molecules in different orientations. It turns out that at larger surface coverage, the adsorption rate decreases, i.e., the apparent diffusion coefficients are smaller. This deceleration can be explained by the transition of molecules adsorbed in a state of larger molar surface area into a state with smaller molar surface area.
Thermal Properties, Isothermal Decomposition by Direct Analysis in Real-Time-of-Flight Mass Spectrometry and Non Isothermal Crystallization Kinetics of Poly(Ethylene-co-Vinyl Alcohol)/Poly(ε-Caprolactone) Blend
Authors: Alghamdi Abdulaziz , Alattas Hussain , Saeed Waseem , Al-Odayni Abdel-Basit , Ahmed Ahmed , Al-Owais Ahmad , Aouak Taieb
Published: March 2021

A series of poly(ethylene-co-vinyl alcohol)/poly(ε-caprolactone) blends with different compositions were prepared using solvent casting. The miscibility of this pair of polymers was investigated using differential scanning calorimetry (DSC), and proved by a negative Flory interaction parameter value calculated from the Nishi–Wang equation. The miscibility of this blend was also confirmed by scanning electronic microscopy (SEM). The thermal behaviors of the obtained materials were investigated by DSC, thermogravimetric analysis, and direct analysis in real-time–time-of-flight mass spectrometry and the results obtained were very relevant. Furthermore, the crystalline properties of the obtained materials were studied by DSC and X-ray diffraction where the Ozawa approach was adopted to investigate the non-isothermal crystallization kinetics. The results obtained revealed that this approach described the crystallization process well.
Multicenter Study of the Seroprevalence of Antibodies against Covid-19 in Patients with Lymphoma: An Analysis of the Oncological Group for the Treatment and Study of Lymphomas (Gotel)
Authors: Franco Fernando , Guirado María , Martínez-Banaclocha Natividad , Gumà Josep , Lavernia Javier , Gómez-Codina José , Rodriguez-Abreu Delvys , Martínez Fani , Barrajón Enrique , Méndez Miriam , Calvo Virginia , Provencio Mariano
Published: March 2021

The new Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) coronavirus has generated a pandemic, in which there are population groups at higher risk and who are potentially fatal victims of the disease. Cancer patients have been considered a group with special susceptibility, particularly patients with lung tumour involvement and haematological neoplasms. The Spanish Lymphoma Oncology Group (GOTEL) carried out a multicenter study of SARS-CoV-2 seroprevalence in patients with lymphoma. Results: A total of 150 patients were included between 22 May and 11 June 2020. The mean age was 65 years (range 17–89), 70 women (46.5%) and 80 men (53, 5%). At the time of diagnosis of lymphoma, 13 cases were stage I (9%), 27 (18%) stage II, 37 (24.5%) stage III, and 73 (48.5%) stage IV, while 6.6% had a primary extranodal origin. A total of 10 cases with positive serology for SARS-CoV-2 were identified, which is a prevalence of 6% in this population. None of the patients required intensive care unit management and all fully recovered from the infection. Conclusion: IgG antibody seroprevalence in lymphoma patients appears similar to that of the general population and does not show greater aggressiveness.
Evaluating the Indirect Costs of Care Associated with Salvage Chemotherapy for Relapsed and Refractory Aggressive-Histology Lymphoma: A Subset Analysis of the Canadian Cancer Trials Group (CCTG) LY.12 Clinical Trial
Authors: Prica Anca , Hay Annette E. , Crump Michael , Mittmann Nicole , Shepherd Lois E. , Meyer Ralph M. , Imrie Kevin I. , Risebrough Nancy , Djurfeldt Marina , Chen Bingshu E. , Cheung Matthew C.
Published: March 2021

We conducted an analysis of indirect costs alongside the LY.12 randomized trial in patients with relapsed or refractory (R/R) aggressive non-Hodgkin lymphoma (NHL). Lost productivity data for Canadian patients and caregivers in the trial were collected at baseline and with each chemotherapy cycle pre-transplant, using an adapted Lost Productivity questionnaire. Mean per patient indirect costs were CAD 2999 for patients in the GDP arm and CAD 3400 in the DHAP arm. A substantial majority was not working or had to reduce their workload during this treatment time. Salvage chemotherapy for R/R aggressive NHL is associated with significant indirect costs to patients and their caregivers.
Comparison of Perioperative Chemotherapy versus Postoperative Chemoradiotherapy for Operable Stomach Cancer: A Western Canadian Province Experience
Authors: Zaidi Adnan , Khan Amal , Duval Claire , Haider Kamal , Ahmed Osama , Dueck Dorie-Anna , Brunet Bryan , Gardiner Donald , Ahmed Shahid
Published: March 2021

Background: The standard approaches for resectable stomach cancer are postoperative chemoradiotherapy (PCR) or perioperative chemotherapy (PC). Limited evidence is available regarding the superiority of one of the two approaches. We aimed to compare the survival of patients with operable stomach cancer who were treated with PC or PCR. Methods: In this retrospective cohort study, patients with operable stomach cancer diagnosed between 2005–2015 in the province of Saskatchewan were identified and, based on type of treatment, were placed into PCR and PC groups. A Cox proportional multivariate analysis was performed to assess independent prognostic variables, including survival advantage of PC over PCR. Results: A total of 88 eligible patients with a median age of 66 (56–71) and a male to female ratio of 1:0.44 were identified. Seventy-three (83%) patients had pathologically node positive disease. Sixty-seven (76%) patients received PCR, while 21 (24%) patients received PC. The median overall survival of the whole group was 34 months, with 38 months (95% CI 24.6–51.3) in the PCR group vs. 30 months (14.3–45.7) in the PC group (p = 0.29). Median relapse-free survival was 34 months (20.7–47.3) in the PCR group vs. 23 months (6.7–39.3) in the PC group (p = 0.20). Toxicities were comparable. On multivariate analysis, T ≥ 3 tumor (HR, 3.57 (1.39–8.56)), neutrophil to lymphocyte ratio (LNR) > 2.8 (HR, 1.85 (1.05–3.25)), and positive resection margins (HR, 1.89 (1.06–3.37)) were independently correlated with inferior survival. Conclusions: This well-designed population based cohort study suggests a lack of survival benefit of PC over PCR. Both treatment options remain viable approaches for resectable stomach cancer.
Rice Plant–Soil Microbiome Interactions Driven by Root and Shoot Biomass
Authors: Fernández-Baca Cristina P. , Rivers Adam R. , Maul Jude E. , Kim Woojae , Poudel Ravin , McClung Anna M. , Roberts Daniel P. , Reddy Vangimalla R. , Barnaby Jinyoung Y.
Published: March 2021

Plant–soil microbe interactions are complex and affected by many factors including soil type, edaphic conditions, plant genotype and phenotype, and developmental stage. The rice rhizosphere microbial community composition of nine recombinant inbred lines (RILs) and their parents, Francis and Rondo, segregating for root and shoot biomass, was determined using metagenomic sequencing as a means to examine how biomass phenotype influences the rhizosphere community. Two plant developmental stages were studied, heading and physiological maturity, based on root and shoot biomass growth patterns across the selected genotypes. We used partial least squares (PLS) regression analysis to examine plant trait-driven microbial populations and identify microbial species, functions, and genes corresponding to root and shoot biomass as well as developmental stage patterns. Species identified correlated with increases in either root or shoot biomass were widely present in soil and included species involved in nitrogen cycling (Anaeromyxobacter spp.) and methane production (Methanocellaavoryzae), as well as known endophytes (Bradyrhizobium spp.). Additionally, PLS analysis allowed us to explore the relationship of developmental stage with species, microbial functions, and genes. Many of the community functions and genes observed during the heading stage were representative of cell growth (e.g., carbohydrate and nitrogen metabolism), while functions correlated with physiological maturity were indicative of cell decay. These results are consistent with the hypothesis that microbial communities exist whose metabolic and gene functions correspond to plant biomass traits.
Effects of Pure and Mixed Pine and Oak Forest Stands on Carabid Beetles
Authors: Wehnert Alexandra , Wagner Sven , Huth Franka
Published: March 2021

The multiple-use approach to forestry applied in Germany aims to combine timber production and habitat management by preserving specific stand structures. We selected four forest stand types comprising (i) pure oak, (ii) equal oak–pine mixtures, (iii) single tree admixtures of oak in pine forest and (iv) pure pine. We analysed the effects of stand composition parameters on species representative of the larger carabid beetles (Carabus arvensis, C. coriaceus, C. hortensis, C. violaceus, Calosoma inquisitor). The main statistical methods used were correlation analyses and generalised linear mixed models. Cal. inquisitor was observed in pure oak forests exclusively. C. coriaceus and C. hortensis were absent from pure pine stands. High activity densities of C. arvensis and C. violaceus were observed in all four forest types. When assessed at the smaller scales of species crown cover proportions and spatial tree species effect zones, C. hortensis was found to be positively related to oak trees with a regular spatial distribution, whereas C. coriaceus preferred lower and more aggregated oak tree proportions. C. violaceus showed strong sex-specific tree species affinities. Information about preferences of carabid beetles is necessary for management activities targeting the adaptation of forest structures to habitat requirements.
The Chemoautotrophically Based Movile Cave Groundwater Ecosystem, a Hotspot of Subterranean Biodiversity
Authors: Brad Traian , Iepure Sanda , Sarbu Serban
Published: March 2021

Movile Cave hosts one of the world’s most diverse subsurface invertebrate communities. In the absence of matter and energy input from the surface, this ecosystem relies entirely on in situ primary productivity by chemoautotrophic microorganisms. The energy source for these microorganisms is the oxidation of hydrogen sulfide provided continuously from the deep thermomineral aquifer, alongside methane, and ammonium. The microbial biofilms that cover the water surface, the cave walls, and the sediments, along with the free-swimming microorganisms, represent the food that protists, rotifers, nematodes, gastropods, and crustacean rely on. Voracious water-scorpions, leeches, and planarians form the peak of the aquatic food web in Movile Cave. The terrestrial community is even more diverse. It is composed of various species of worms, isopods, pseudoscorpions, spiders, centipedes, millipedes, springtails, diplurans, and beetles. An updated list of invertebrate species thriving in Movile Cave is provided herein. With 52 invertebrate species (21 aquatic and 31 terrestrial), of which 37 are endemic for this unusual, but fascinating environment, Movile Cave is the first known chemosynthesis-based groundwater ecosystem. Therefore, Movile Cave deserves stringent attention and protection.
Tools for Remote Exploration: A Lithium (Li) Dedicated Spectral Library of the Fregeneda–Almendra Aplite–Pegmatite Field
Authors: Cardoso-Fernandes Joana , Silva João , Dias Filipa , Lima Alexandre , Teodoro Ana C. , Barrès Odile , Cauzid Jean , Perrotta Mônica , Roda-Robles Encarnación , Ribeiro Maria Anjos
Published: March 2021

The existence of diagnostic features in the visible and infrared regions makes it possible to use reflectance spectra not only to identify mineral assemblages but also for calibration and classification of satellite images, considering lithological and/or mineral mapping. For this purpose, a consistent spectral library with the target spectra of minerals and rocks is needed. Currently, there is big market pressure for raw materials including lithium (Li) that has driven new satellite image applications for Li exploration. However, there are no reference spectra for petalite (a Li mineral) in large, open spectral datasets. In this work, a spectral library was built exclusively dedicated to Li minerals and Li pegmatite exploration through satellite remote sensing. The database includes field and laboratory spectra collected in the Fregeneda–Almendra region (Spain–Portugal) from (i) distinct Li minerals (spodumene, petalite, lepidolite); (ii) several Li pegmatites and other outcropping lithologies to allow satellite-based lithological mapping; (iii) areas previously misclassified as Li pegmatites using machine learning algorithms to allow comparisons between these regions and the target areas. Ancillary data include (i) sample location and coordinates, (ii) sample conditions, (iii) sample color, (iv) type of face measured, (v) equipment used, and for the laboratory spectra, (vi) sample photographs, (vii) continuum removed spectra files, and (viii) statistics on the main absorption features automatically extracted. The potential future uses of this spectral library are reinforced by its major advantages: (i) data is provided in a universal file format; (ii) it allows users to compare field and laboratory spectra; (iii) a large number of complementary data allow the comparison of shape, asymmetry, and depth of the absorption features of the distinct Li minerals.
Atypical Presentation of Bacteremic Urinary Tract Infection in Older Patients: Frequency and Prognostic Impact
Authors: Laborde Caroline , Bador Julien , Hacquin Arthur , Barben Jérémy , Putot Sophie , Manckoundia Patrick , Putot Alain
Published: March 2021

In older patients, urinary tract infection (UTI) often has an atypical clinical presentation, making its diagnosis difficult. We aimed to describe the clinical presentation in older inpatients with UTI-related bacteremia and to determine the prognostic impact of atypical presentation. This cohort study included all consecutive patients older than 75 years hospitalized in a university hospital in 2019 with a UTI-related gram-negative bacillus (GNB) bacteremia, defined by blood and urine cultures positive for the same GNB, and followed up for 90 days. Patients with typical symptoms of UTI were compared to patients with atypical forms. Among 3865 inpatients over 75 with GNB-positive urine culture over the inclusion period, 105 patients (2.7%) with bacteremic UTI were included (mean age 85.3 ± 5.9, 61.9% female). Among them, UTI symptoms were reported in only 38 patients (36.2%) and 44 patients (41.9%) had no fever on initial management. Initial diagnosis of UTI was made in only 58% of patient. Mortality at 90 days was 23.6%. After adjustment for confounders, hyperthermia (HR = 0.37; IC95 (0.14–0.97)) and early UTI diagnosis (HR = 0.35; IC95 (0.13–0.94)) were associated with lower mortality, while UTI symptoms were not associated with prognosis. In conclusion, only one third of older patients with UTI developing bacteremia had UTI symptoms. However, early UTI diagnosis was associated with better survival.
Dielectric Properties of Ovine Heart at Microwave Frequencies
Authors: Ištuk Niko , Porter Emily , O’Loughlin Declan , McDermott Barry , Santorelli Adam , Abedi Soroush , Joachimowicz Nadine , Roussel Hélène , O’Halloran Martin
Published: March 2021

Accurate knowledge of the dielectric properties of biological tissues is important in dosimetry studies and for medical diagnostic, monitoring and therapeutic technologies. In particular, the dielectric properties of the heart are used in numerical simulations of radiofrequency and microwave heart ablation. In one recent study, it was demonstrated that the dielectric properties of different components of the heart can vary considerably, contrary to previous literature that treated the heart as a homogeneous organ with measurements that ignored the anatomical location. Therefore, in this study, we record and report the dielectric properties of the heart as a heterogeneous organ. We measured the dielectric properties at different locations inside and outside of the heart over the 500 MHz to 20 GHz frequency range. Different parts of the heart were identified based on the anatomy of the heart and their function; they include the epicardium, endocardium, myocardium, exterior and interior surfaces of atrial appendage, and the luminal surface of the great vessels. The measured dielectric properties for each part of the heart are reported at both a single frequency (2.4 GHz), which is of interest in microwave medical applications, and as parameters of a broadband Debye model. The results show that in terms of dielectric properties, different parts of the heart should not be considered the same, with more than 25% difference in dielectric properties between some parts. The specific Debye models and single frequency dielectric properties from this study can be used to develop more detailed models of the heart to be used in electromagnetic modeling.
A Full-Newton AC-DC Power Flow Methodology for HVDC Multi-Terminal Systems and Generic DC Network Representation
Authors: Vasconcelos Leandro Almeida , Passos Filho João Alberto , Marcato André Luis Marques , Junqueira Giovani Santiago
Published: March 2021

The use of Direct Current (DC) transmission links in power systems is increasing continuously. Thus, it is important to develop new techniques to model the inclusion of these devices in network analysis, in order to allow studies of the operation and expansion planning of large-scale electric power systems. In this context, the main objective of this paper is to present a new methodology for a simultaneous AC-DC power flow for a multi-terminal High Voltage Direct Current (HVDC) system with a generic representation of the DC network. The proposed methodology is based on a full Newton formulation for solving the AC-DC power flow problem. Equations representing the converters and steady-state control strategies are included in a power flow problem formulation, resulting in an expanded Jacobian matrix of the Newton method. Some results are presented based on HVDC test systems to confirm the effectiveness of the proposed approach.
Optimal Adaptive Gain LQR-Based Energy Management Strategy for Battery–Supercapacitor Hybrid Power System
Authors: Ferahtia Seydali , Djeroui Ali , Mesbahi Tedjani , Houari Azeddine , Zeghlache Samir , Rezk Hegazy , Paul Théophile
Published: March 2021

This paper aims at presenting an energy management strategy (EMS) based upon optimal control theory for a battery–supercapacitor hybrid power system. The hybrid power system consists of a lithium-ion battery and a supercapacitor with associated bidirectional DC/DC converters. The proposed EMS aims at computing adaptive gains using the salp swarm algorithm and load following control technique to assign the power reference for both the supercapacitor and the battery while achieving optimal performance and stable voltage. The DC/DC converter model is derived utilizing the first-principles method and computes the required gains to achieve the desired power. The fact that the developed algorithm takes disturbances into account increases the power elements’ life expectancies and supplies the power system with the required power.
Catalyzed Ethanol Chemical Looping Gasification Mechanism on the Perfect and Reduced Fe2O3 Surfaces
Authors: Luo Laixing , Zheng Xing , Wang Jianye , Qin Wu , Xiao Xianbin , Zheng Zongming
Published: March 2021

Biomass chemical looping gasification (CLG) is a novel gasification technology for hydrogen production, where the oxygen carrier (OC) transfers lattice oxygen to catalytically oxidize fuel into syngas. However, the OC is gradually reduced, showing different reaction activities in the CLG process. Fully understanding the CLG reaction mechanism of fuel molecules on perfect and reduced OC surfaces is necessary, for which the CLG of ethanol using Fe2O3 as the OC was introduced as the probe reaction to perform density functional theory calculations to reveal the decomposition mechanism of ethanol into the synthesis gas (including H2, CH4, ethylene, formaldehyde, acetaldehyde, and CO) on perfect and reduced Fe2O3(001) surfaces. When Fe2O3(001) is reduced to FeO0.375(001), the calculated barrier energy decreases and then increases again, suggesting that the reduction state around FeO(001) favors the catalytic decomposition of ethanol to produce hydrogen, which proves that the degree of reduction has an important effect on the CLG reaction.
Energy Efficiency Analysis of the Refining Unit in Thermo-Mechanical Pulp Mill
Authors: Talebjedi Behnam , Laukkanen Timo , Holmberg Henrik , Vakkilainen Esa , Syri Sanna
Published: March 2021

A refining model is developed to analyses the refining process’s energy efficiency based on the refining variables. A simulation model is obtained for longer-term refining energy analysis by further developing the MATLAB Thermo-Mechanical Pulping Simulink toolbox. This model is utilized to predict two essential variables for refining energy efficiency calculation: refining motor-load and generated steam. The conventional variable for presenting refining energy efficiency is refining specific energy consumption (RSEC), which is the ratio of the refining motor load to throughput and does not consider the share of recovered energy from the refining produced steam. In this study, a new variable, corrected refining specific energy consumption (CRSEC), is introduced and practiced for better representation of the refining energy efficiency. In the calculation process of the CRSEC, recovered energy from the refining generated steam is considered useful energy. The developed model results in 160% and 78.75% improvement in simulation model determination coefficient and error, respectively. Utilizing the developed model and hourly district heating demand for CRSEC calculation, results prove a 22% annual average difference between CRSEC and RSEC. Findings confirm that the wintertime refining energy efficiency is 27% higher due to higher recovered energy in the heat recovery unit compared to summertime.
Groundwater Risk Assessment in the Context of an Underground Coal Mine Closure and an Economic Evaluation of Proposed Treatments: A Case Study
Authors: Fidalgo Valverde Gregorio , Duda Adam , Iglesias Rodríguez Francisco Javier , Frejowski Aleksander , Todorov Ivan
Published: March 2021

Mining companies are responsible for the impacts that result from their mining activities even after the mining period has ended. At the same time, at the European and international levels, there is a lack of a detailed operational methodology comprising environmental risks during and after closure of underground coal mines. The environmental risk aspects that need to be considered when planning the closure of an underground coal mine and post closure in the broader environmental context are the following: modification of water flow scheme, surface instability, mine gas emission on the surface, and water and soil pollution. In this study, we focus on assessing groundwater risk in the context of an underground coal mine closure and evaluating the selected risk mitigation strategies in terms of performance and cost. The results from this study could be used for developing a final closure groundwater assessment plan by selecting the most feasible treatment alternatives for different environmental impacts, together with the transitional monitoring that could guarantee a hazard level in compliance with land reuse and the use of natural resources. Finally, the cost-efficient monitoring and treatment programs are used to estimate the financial provisions needed to mitigate groundwater risks during underground coal mine closure contexts.
Optimized Fuzzy Skyhook Control for Semi-Active Vehicle Suspension with New Inverse Model of Magnetorheological Fluid Damper
Authors: Ma Teng , Bi Fengrong , Wang Xu , Tian Congfeng , Lin Jiewei , Wang Jie , Pang Gejun
Published: March 2021

To improve the performance of vehicle suspension, this paper proposes a semi-active vehicle suspension with a magnetorheological fluid (MRF) damper. We designed an optimized fuzzy skyhook controller with grey wolf optimizer (GWO) algorithm base on a new neuro-inverse model of the MRF damper. Because the inverse model of the MRF damper is difficult to establish directly, the Elman neural network was applied. The novelty of this study is the application of the new inverse model for semi-active vibration control and optimization of the semi-active suspension control method. The calculation results showed that the new inverse model can accurately calculate the required control current. The fuzzy skyhook control method optimized by the grey wolf optimizer (GWO) algorithm was established based on the inverse model to control the suspension vibration. The simulation results showed that the optimized fuzzy skyhook control method can simultaneously reduce the amplitude of vertical acceleration, suspension deflection, and tire dynamic load.
Development of a Software Tool for Seismic Probabilistic Safety Assessment Quantification with a Sufficiently Large Number of Bins for Enhanced Accuracy
Authors: Kim Ji Suk , Kim Man Cheol
Published: March 2021

Quantification in seismic probabilistic safety assessment (PSA) includes the convolution of hazard and fragility curves. Because it is difficult to find the closed-form integration for the convolution of two curves, numerical methods are widely used in practice. In practical applications, the number of ground motion level bins in numerical methods is limited, and it is not clear whether the limited number of bins leads to conservative or optimistic results. In this study, the effect of the number of bins on the quantification results with simplified assumptions is investigated. It is found that the quantification results mostly decrease as the number of bins increases. To enhance accuracy in the quantification results of seismic PSA, a method and a software tool that enable a sufficiently large number of bins to be used for the quantification of seismic PSA models are developed. The application of the developed software tool to an example seismic PSA model demonstrates how the quantification results approach accurate results as the number of bins increases. The software tool developed in this study is expected to enhance the accuracy of seismic PSA quantification results.
Stable Isotopes in Tree Rings of Pinus heldreichii Can Indicate Climate Variability over the Eastern Mediterranean Region
Authors: Lukač Ljubica , Mikac Stjepan , Urban Otmar , Kolář Tomáš , Rybníček Michal , Ač Alexander , Trnka Mirek , Marek Michal V.
Published: March 2021

A long-term context is important for understanding past climatic variability. Although tree-ring widths (TRWs) are widely used as a proxy for reconstructing past climate, the use of annually-resolved values of δ13C and δ18O tree-ring stable isotopes (TRSIs) is increasing and may provide further valuable information. Here, we present a 487-year-long TRW chronology and 240-year-long TRSI chronology for Bosnian pine (Pinus heldreichii H. Christ) and compare them to each other. We demonstrate that both δ13C and δ18O values are better proxies for temperature, precipitation, and drought than TRW. The correlations between these climate parameters and TRSIs are strongest for the combined summer (JJA) period. The results of temporal and spatial field correlation indicate that TRSI chronologies are stable, reliable proxies for JJA precipitation reconstruction over the whole Balkan Peninsula and surrounding eastern Mediterranean region. However, the stability of the temperature signal of the both δ13C and δ18O chronologies declines after the 1950s. Our work supports the emerging evidence that TRSI data track climate variability more accurately than a conventional TRW approach and can be subsequently used for the reconstruction of past climate.
Editorial for Special Issue “Biodiversity and Management of Temperate Floodplain Forests”
Authors: Machar Ivo
Published: March 2021

Floodplain forests are considered as key forest ecosystems in lowland regions of the temperate climate zone [...]
Nitrogen Recovery from Enhanced Efficiency Fertilizers and Urea in Intensively Managed Black Walnut (Juglans nigra) Plantations
Authors: Sloan Joshua L. , Salifu Francis K. , Jacobs Douglass F.
Published: March 2021

Intensively managed forest plantations often require fertilization to maintain site fertility and to improve growth and yield over successive rotations. We applied urea-based “enhanced-efficiency fertilizers” (EEF) containing 0.5 atom% 15N at a rate of 224 kg N ha−1 to soils under mid-rotation black walnut (Juglans nigra L.) plantations to track the fate of applied 15N within aboveground ecosystem components during the 12-month period after application. Treatments included Agrotain Ultra (urea coated with a urease inhibitor), Arborite EC (urea coated with water-soluble boron and phosphate), Agrium ESN (polymer-coated urea), uncoated urea, and an unfertilized control. Agrotain Ultra and Arborite EC increased N concentrations of competing vegetation within one month after fertilization, while neither Agrium ESN nor uncoated urea had any effect on competing vegetation N concentrations during the experiment. Agrotain Ultra and Arborite EC increased δ15N values in leaves of crop trees above those of controls at one and two months after fertilization, respectively. By contrast, Agrium ESN and uncoated urea had no effect on δ15N values in leaves of crop trees until three months after fertilization. Fertilizer N recovery (FNR) varied among ecosystem components, with competing vegetation acting as a sink for applied nutrients. There were no significant differences in FNR for all the urea-based EEF products compared to uncoated urea. Agrium ESN was the only EEF that exhibited controlled-release activity in this study, with other fertilizers behaving similarly to uncoated urea.
Wind-Forced Submesoscale Symmetric Instability around Deep Convection in the Northwestern Mediterranean Sea
Authors: Bosse Anthony , Testor Pierre , Damien Pierre , Estournel Claude , Marsaleix Patrick , Mortier Laurent , Prieur Louis , Taillandier Vincent
Published: March 2021

During the winter from 2009 to 2013, the mixed layer reached the seafloor at about 2500 m in the northwestern Mediterranean Sea. Intense fronts around the deep convection area were repeatedly sampled by autonomous gliders. Subduction down to 200–300 m, sometimes deeper, below the mixed layer was regularly observed testifying of important frontal vertical movements. Potential Vorticity dynamics was diagnosed using glider observations and a high resolution realistic model at 1-km resolution. During down-front wind events in winter, remarkable layers of negative PV were observed in the upper 100 m on the dense side of fronts surrounding the deep convection area and successfully reproduced by the numerical model. Under such conditions, symmetric instability can grow and overturn water along isopycnals within typically 1–5 km cross-frontal slanted cells. Two important hotpspots for the destruction of PV along the topographically-steered Northern Current undergoing frequent down-front winds have been identified in the western part of Gulf of Lion and Ligurian Sea. Fronts were there symmetrically unstable for up to 30 days per winter in the model, whereas localized instability events were found in the open sea, mostly influenced by mesoscale variability. The associated vertical circulations also had an important signature on oxygen and fluorescence, highlighting their under important role for the ventilation of intermediate layers, phytoplankton growth and carbon export.
The Effects of Artificially Dosed Adult Rumen Contents on Abomasum Transcriptome and Associated Microbial Community Structure in Calves
Authors: Gaowa Naren , Li Wenli , Murphy Brianna , Cox Madison S.
Published: March 2021

This study aimed to investigate the changes in abomasum transcriptome and the associated microbial community structure in young calves with artificially dosed, adult rumen contents. Eight young bull calves were randomly dosed with freshly extracted rumen contents from an adult cow (high efficiency (HE), n = 4), or sterilized rumen content (Con, n = 4). The dosing was administered within 3 days of birth, then at 2, 4, and 6 weeks following the initial dosing. Abomasum tissues were collected immediately after sacrifice at 8 weeks of age. Five genera (Tannerella, Desulfovibrio, Deinococcus, Leptotrichia, and Eubacterium; p < 0.05) showed significant difference in abundance between the treatments. A total of 975 differentially expressed genes were identified (p < 0.05, fold-change > 1.5, mean read-counts > 5). Pathway analysis indicated that up-regulated genes were involved in immune system process and defense response to virus, while the down-regulated genes involved in ion transport, ATP biosynthetic process, and mitochondrial electron transport. Positive correlation (r > 0.7, p < 0.05) was observed between TRPM4 gene and Desulfovibrio, which was significantly higher in the HE group. TRPM4 had a reported role in the immune system process. In conclusion, the dosing of adult rumen contents to calves can alter not only the composition of active microorganisms in the abomasum but also the molecular mechanisms in the abomasum tissue, including reduced protease secretion and decreased hydrochloric acid secretion.
Potential Impacts of Interleukin-17A Promoter Polymorphisms on the EGFR Mutation Status and Progression of Non-Small Cell Lung Cancer in Taiwan
Authors: Lee Kai-Ling , Lai Tsung-Ching , Wang Yao-Chen , Shih Pei-Chun , Yang Yi-Chieh , Tsao Thomas Chang-Yao , Liu Tu-Chen , Wen Yu-Ching , Chang Lun-Ching , Yang Shun-Fa , Chien Ming-Hsien
Published: March 2021

Non-small cell lung cancer (NSCLC) is a typical inflammation-associated cancer, and lung adenocarcinoma (LUAD) is the most common histopathological subtype. Epidermal growth factor receptor (EGFR) mutations are the most common driver mutations of LUAD, and they have been identified as important therapeutic targets by EGFR tyrosine kinase inhibitors. Interleukin (IL)-17A secreted by T-helper 17 lymphocytes is a proinflammatory cytokine that plays an important role in cancer pathogenesis. The present study was designed to investigate the possible associations among IL-17A genetic polymorphisms, EGFR mutation status, and the clinicopathologic development of LUAD in a Taiwanese population. Our study population consisted of 277 LUAD patients harboring the wild-type (WT) EGFR or a mutant (MT) EGFR. Four single-nucleotide polymorphisms (SNPs) of IL-17A in the peripheral blood, including rs8193036(C > T), rs8193037(G > A), rs2275913(G > A), and rs3748067(C > T) loci, were genotyped using a TaqMan allelic discrimination assay. Our results showed that none of these IL-17A SNPs were correlated with the risk of developing mutant EGFR. However, patients with a smoking habit who carried the GA genotype of IL-17A rs8193037 had a significantly lower susceptibility to EGFR mutations (adjusted odds ratio (AOR): 0.225; 95% confidence interval (CI): 0.056~0.900, p = 0.035). Moreover, compared to individuals carrying the CC genotype of rs8193036 at IL-17A, T-allele carriers (CT + TT) were at higher risk of developing more-advanced stages (stage III or IV; p = 0.020). In the WT EGFR subgroup analysis, IL-17A rs8193036 T-allele carriers had higher risks of developing an advanced tumor stage (p = 0.016) and lymphatic invasion (p = 0.049). Further analyses of clinical datasets revealed correlations of IL-17 receptor A (IL-17RA) and IL-17RC expressions with a poor prognosis of LUAD patients with a smoking history or with higher levels of tumor-infiltrating lymphocytes. In conclusion, our results suggested that two functional promoter polymorphisms of IL-17A, i.e., rs8193036 and rs8193037, were associated with the EGFR mutation status and progression in LUAD patients, indicating that these two genetic variants might act as possible markers for predicting patients’ clinical prognoses.
Shared Ancestry and Signatures of Recent Selection in Gotland Sheep
Authors: Ghoreishifar Seyed Mohammad , Rochus Christina Marie , Moghaddaszadeh-Ahrabi Sima , Davoudi Pourya , Salek Ardestani Siavash , Zinovieva Natalia A. , Deniskova Tatiana E. , Johansson Anna M.
Published: March 2021

Gotland sheep, a breed native to Gotland, Sweden (an island in the Baltic Sea), split from the Gute sheep breed approximately 100 years ago, and since, has probably been crossed with other breeds. This breed has recently gained popularity, due to its pelt quality. This study estimates the shared ancestors and identifies recent selection signatures in Gotland sheep using 600 K single nucleotide polymorphism (SNP) genotype data. Admixture analysis shows that the Gotland sheep is a distinct breed, but also has shared ancestral genomic components with Gute (~50%), Karakul (~30%), Romanov (~20%), and Fjällnäs (~10%) sheep breeds. Two complementary methods were applied to detect selection signatures: A Bayesian population differentiation FST and an integrated haplotype homozygosity score (iHS). Our results find that seven significant SNPs (q-value < 0.05) using the FST analysis and 55 significant SNPs (p-value < 0.0001) using the iHS analysis. Of the candidate genes that contain significant markers, or are in proximity to them, we identify several belongings to the keratin genes, RXFP2, ADCY1, ENOX1, USF2, COX7A1, ARHGAP28, CRYBB2, CAPNS1, FMO3, and GREB1. These genes are involved in wool quality, polled and horned phenotypes, fertility, twining rate, meat quality, and growth traits. In summary, our results provide shared founders of Gotland sheep and insight into genomic regions maintained under selection after the breed was formed. These results contribute to the detection of candidate genes and QTLs underlying economic traits in sheep.
The Effect of a Points System on Incentivizing Academic Behaviors of Attending Ophthalmologists
Authors: Chen Darren A. , Cohen Emily , Lelli Gary J.
Published: March 2021

(1) Background: Little is known regarding the best ways to promote academic throughput within the ranks of attending ophthalmology physicians. The purpose of this project is to evaluate the effect of a monetized points system on incentivizing research output and other academic activity in academic ophthalmology attendings. (2) Methods: This is a retrospective study of 15 academic ophthalmology attendings at a single academic teaching hospital from 1 July 2015 to 30 June 2020. A points system was implemented in the 2017 academic year (1 July 2016–30 June 2017), in which ophthalmology attendings accrued points for eight categories of academic achievement. We compared the overall number of publications, number of first/senior author publications, and corresponding impact factors of journals via the PubMed database in the two years of data before and after the points system was implemented. We analyzed points awarded for eight categories of academic achievement in the first, second, and third year of the program. (3) Results: There was no significant change in research productivity for attending ophthalmologists after institution of the points system. From 2017 to 2019, Mann–Whitney analysis revealed a significant increase in points awarded for mentorship per physician (p = 0.013). (4) Conclusions: Our data suggest that within the framework of the points system, attendings—rather than prioritizing publications—gravitated towards mentorship activities to accrue points.
Ciguatera Mini Review: 21st Century Environmental Challenges and the Interdisciplinary Research Efforts Rising to Meet Them
Authors: Loeffler Christopher R. , Tartaglione Luciana , Friedemann Miriam , Spielmeyer Astrid , Kappenstein Oliver , Bodi Dorina
Published: March 2021

Globally, the livelihoods of over a billion people are affected by changes to marine ecosystems, both structurally and systematically. Resources and ecosystem services, provided by the marine environment, contribute nutrition, income, and health benefits for communities. One threat to these securities is ciguatera poisoning; worldwide, the most commonly reported non-bacterial seafood-related illness. Ciguatera is caused by the consumption of (primarily) finfish contaminated with ciguatoxins, potent neurotoxins produced by benthic single-cell microalgae. When consumed, ciguatoxins are biotransformed and can bioaccumulate throughout the food-web via complex pathways. Ciguatera-derived food insecurity is particularly extreme for small island-nations, where fear of intoxication can lead to fishing restrictions by region, species, or size. Exacerbating these complexities are anthropogenic or natural changes occurring in global marine habitats, e.g., climate change, greenhouse-gas induced physical oceanic changes, overfishing, invasive species, and even the international seafood trade. Here we provide an overview of the challenges and opportunities of the 21st century regarding the many facets of ciguatera, including the complex nature of this illness, the biological/environmental factors affecting the causative organisms, their toxins, vectors, detection methods, human-health oriented responses, and ultimately an outlook towards the future. Ciguatera research efforts face many social and environmental challenges this century. However, several future-oriented goals are within reach, including digital solutions for seafood supply chains, identifying novel compounds and methods with the potential for advanced diagnostics, treatments, and prediction capabilities. The advances described herein provide confidence that the tools are now available to answer many of the remaining questions surrounding ciguatera and therefore protection measures can become more accurate and routine.
Conversations in Times of Isolation: Exploring Rural-Dwelling Older Adults’ Experiences of Isolation and Loneliness during the COVID-19 Pandemic in Manitoba, Canada
Authors: Herron Rachel V. , Newall Nancy E. G. , Lawrence Breanna C. , Ramsey Doug , Waddell Candice M. , Dauphinais Jennifer
Published: March 2021

Older adults have been described as a vulnerable group in the current context of the COVID-19 pandemic. In Canada, where this study took place, older adults have been encouraged to self-isolate while the rest of the population has been cautioned against in-person contact with them. Prior to COVID-19, social isolation and loneliness among older adults was considered a serious public health concern. Using a series of semi-structured interviews with 26 community-dwelling older adults (65+) living in rural Manitoba, we explore older adults’ experiences of isolation and loneliness in the initial stages of the pandemic between the months of May and July 2020. Participants identified a loss of autonomy, loss of activities and social spaces (e.g., having coffee or eating out, volunteering, and going to church), and lack of meaningful connection at home as factors influencing their sense of isolation and loneliness. Although these loses initially influenced participants’ self-reported isolation and loneliness, the majority developed strategies to mitigate isolation and loneliness, such as drawing on past experiences of isolation, engaging in physically distanced visits, connecting remotely, and “keeping busy.” Our findings call attention to the role of different environments and resources in supporting older adults social and emotional wellbeing, particularly as they adapt to changes in social contact over time.
Association of Temporomandibular Disorder Symptoms with Physical Fitness among Finnish Conscripts
Authors: Miettinen Ossi , Kämppi Antti , Tanner Tarja , Anttonen Vuokko , Patinen Pertti , Päkkilä Jari , Tjäderhane Leo , Sipilä Kirsi
Published: March 2021

Studies on the role of physical fitness, physical activity and obesity as risk factors for temporomandibular disorders (TMD) are scarce. The aim of the present study was to evaluate the association of TMD symptoms with physical fitness, physical activity and body mass index (BMI) among Finnish conscripts. The study sample consisted of 8685 Finnish conscripts. Data on self-reported TMD symptoms were used as outcome variables. Physical activity (questionnaire), physical fitness (measured with physical tests: Cooper test, push-ups, sit-ups and standing long jump) and body mass index (BMI) were used as explanatory variables. The associations between TMD symptoms and explanatory variables were evaluated using Chi-squared test and logistic regression analysis. The prevalence of all TMD symptoms was significantly higher among those who exercised more rarely than weekly. Pain-related TMD symptoms were also significantly more frequent among those who were overweight (BMI ≥ 25). Poor push-up results and overweight (BMI ≥ 25) were significantly associated with jaw pain and TMJ pain at jaw rest. The present study showed that good physical fitness may be a protective factor against TMD pain. Dentists should also be prepared to motivate TMD patients to physical activity and regular exercise as part of the treatment.
Factors Influencing Utilization of Preventive Health Services in Primary Health Care in the Republic of Serbia
Authors: Mitričević Slavka , Janković Janko , Stamenković Željka , Bjegović-Mikanović Vesna , Savić Marko , Stanisavljević Dejana , Mandić-Rajčević Stefan
Published: March 2021

The use of preventive health services is a long-term health investment due to its potential to help individuals to take care of their health. This study aimed to explore the availability and performance of health services in primary health care (PHC) in the domain of general practice (GP), pediatrics, and gynecology, as well as to analyze the influence of sociodemographic and health determinants on the utilization of preventive health services. This descriptive study used data from the National Health Insurance Fund and the Statistical Office of the Republic of Serbia for 2015 and included 149 independent PHC units. The relationship between the utilization of preventive services and sociodemographic and health characteristics of the population was analyzed by bivariate and multivariate linear regression models. The higher health expenditure per capita and noncommunicable diseases mortality rate were, the more preventive health services were provided by a chosen GP. Children with a higher completion rate of primary school (p = 0.024), higher health expenditure (p = 0.017), and higher life expectancy at birth (p = 0.041) had more preventive health services. The fertility rate was positively associated with the number of preventive health services per 1000 women (p = 0.033). Our findings should serve as a starting point for where efforts should be made to achieve better health outcomes.