bluuebunny/crossref_metadata_2025 huggingface dataset:
Studying Legal Persuasion and Emotion in Spanish and English: An Advocate General’s Dismissal of the Rule-of-law Challenge by Hungary and Poland
Authors: Orts María Ángeles
Published: November 2022

The present work examines the role of persuasive lexicon in legal discourse through the analysis of emotional devices at a lexical and rhetorical level. Our preliminary premise is that emotion is deployed by experts to convey the sentiment of shared values and epistemic trust: the need to rely on the tenets of the law as fair and conducive to the common good. The corpus of our study is constituted by the conclusions in their original Spanish, and their translation into English, by the Advocate General Manuel Campos on the challenge by Hungary and Poland of the regulation establishing a “conditionality regime” in the event of a rule-of-law breach in a EU Member State. To this end, we undertake a two-pronged analysis of legal persuasion to find out what emotional devices are deployed to convey the rule-of-law principles of justice, non-discrimination, equality and solidarity. At a first stage, we concentrate upon the polarity and intensity of emotion words and their categories. Emotional implicatures in the shape of legal technolects and metaphorical ontologies and the way they are deployed in each text constitutes the second part of our study. Among our conclusions are that both the original and target texts have a strong persuasive character, mainly grounded in the negative emotion of fear and the positive emotion of trust, and that there are variances in the emotional language deployed in either version due to the different mechanics of each law system and to functional differences between English and Spanish.
Changepoint detection in non-exchangeable data
Authors: Hallgren Karl L. , Heard Nicholas A. , Adams Niall M.
Published: November 2022

Changepoint models typically assume the data within each segment are independent and identically distributed conditional on some parameters that change across segments. This construction may be inadequate when data are subject to local correlation patterns, often resulting in many more changepoints fitted than preferable. This article proposes a Bayesian changepoint model that relaxes the assumption of exchangeability within segments. The proposed model supposes data within a segment arem-dependent for some unknown$$m \geqslant 0$$m⩾0that may vary between segments, resulting in a model suitable for detecting clear discontinuities in data that are subject to different local temporal correlations. The approach is suited to both continuous and discrete data. A novel reversible jump Markov chain Monte Carlo algorithm is proposed to sample from the model; in particular, a detailed analysis of the parameter space is exploited to build proposals for the orders of dependence. Two applications demonstrate the benefits of the proposed model: computer network monitoring via change detection in count data, and segmentation of financial time series.
Predicates of personal taste and normative meaning
Authors: Zouhar Marián
Published: November 2022

The main aim of the paper is to reject the idea that predicates of personal taste express normative meanings. According to a recent theory proposed by Daniel Gutzmann, predicates of personal taste express both a truth-conditional content and a use-conditional content, the latter being normative. The purported normativity of predicates of personal taste is supposed to consist in that when producing utterances containing such predicates, their speakers suggest how other people ought to experience the objects of taste under discussion. The paper provides a bunch of evidence to show there are utterances containing predicates of personal taste that cannot be used normatively in this sense. In particular, it is claimed that one can make such an utterance and then add a related normative piece of information without doing anything redundant; one can also make such an utterance and then deny the related normative piece of information without generating any contradiction or infelicity. At the same time, it is admitted there are situations in which the speakers do communicate something normative by producing utterances containing predicates of personal taste. We thus need an explanation to cope with such cases. Although the paper does not offer one, it provides some reasons to the effect that a correct explanation has to be pragmatic rather than semantic.
A Study in brown
Authors: Newall Michael
Published: November 2022

Philosophers rarely write in an extended way about particular colors. So, why write about brown? We shall see that an investigation of brown unsettles some established ideas about color in significant ways. In particular, I will (i) explore reasons for thinking that brown is an elementary color, (ii) reassess attitudes in color science that are taken to rule that possibility out, and (iii) present a new reason for rejecting most forms of color realism.
Protective treatments for copper alloy artworks: preliminary studies of sodium oxalate and limewater effectiveness against bronze disease
Authors: Monari Giulia , Galeotti Monica , Matteini Mauro , Salvadori Barbara , Stifanese Roberto , Traverso Pierluigi , Vettori Silvia , Letardi Paola
Published: November 2022

Nantokite (CuCl) locked inside subsurface micro-pits has been recognised as the driving force to the form of corrosion called bronze disease. The use of the traditional corrosion inhibitor benzotriazole is questioned because of toxicity. So there is a need for alternative conservation treatments. This work is focused on the experimental design to test the effectiveness of sodium oxalate followed by treatment with limewater to face bronze disease on outdoor bronzes. A number of foundry bronze coupons were exposed to weathering at Genoa Experimental Marine Station (GEMS) exposure site and sprayed twice a week with a 5% NaCl solution for the first 124 days. After 562 days of natural weathering, the patinas on coupons were characterised with non-destructive techniques (NDT) and the presence of nantokite was verified. We designed a workflow, as similar as possible to conservation treatments on real artworks, to test a 3% w/v sodium oxalate treatment with two different application times, with or without limewater, on the coupons. The effectiveness of the treatments was analysed by comparison of surface properties by several NDT measurements. A statistical approach and XRD measurements directly on the corroded bronze surfaces are suggested as an effective way to characterise and compare the overall behaviour of bronze disease treatments for conservation.
Contemporary Data on the Status and Medical Management of Acute Heart Failure
Authors: Bazmpani Maria Anna , Papanastasiou Christos A. , Kamperidis Vasileios , Zebekakis Pantelis E. , Karvounis Haralambos , Kalogeropoulos Andreas P. , Karamitsos Theodoros D.
Published: November 2022

Purpose of Review

Acute heart failure (AHF) is among the leading causes for unplanned hospital admission. Despite advancements in the management of chronic heart failure, the prognosis of AHF remains poor with high in-hospital mortality and increased rates of unfavorable post-discharge outcomes. With this review, we aim to summarize current data on AHF epidemiology, focus on the different patient profiles and classifications, and discuss management, including novel therapeutic options in this area.

Recent Findings

There is significant heterogeneity among patients admitted for AHF in their baseline characteristics, heart failure (HF) aetiology and precipitating factors leading to decompensation. A novel classification scheme based on four distinct clinical scenarios has been included in the most recent ESC guidelines, in an effort to better risk stratify patients and guide treatment. Intravenous diuretics, vasodilators, and inotropes remain the cornerstone of management in the acute phase, and expansion of use of mechanical circulatory support has been noted in recent years. Meanwhile, many treatments that have proved their value in chronic heart failure demonstrate promising results in the setting of AHF and research in this field is currently ongoing.

Acute heart failure remains a major health challenge with high in-hospital mortality and unfavorable post-discharge outcomes. Admission for acute HF represents a window of opportunity for patients to initiate appropriate treatment as soon as possible after stabilization. Future studies are needed to elucidate which patients will benefit the most by available therapies and define the optimal timing for treatment implementation.
The acquisition of emotion-laden words from childhood to adolescence
Authors: Sabater Lucía , Ponari Marta , Haro Juan , Fernández-Folgueiras Uxia , Moreno Eva M. , Pozo Miguel A. , Ferré Pilar , Hinojosa José A.
Published: November 2022

Studies investigating how children acquire emotional vocabularies have mainly focused on words that describe feelings or affective states (emotion-label words, e.g., joy) trough subjective assessments of the children’s lexicon reported by their parents or teachers. In the current cross-sectional study, we objectively examined the age of acquisition of words that relate to emotions without explicitly referring to affective states (emotion-laden words, e.g., cake, tomb, rainbow) using a picture naming task. Three hundred and sixty participants belonging to 18 age groups from preschool to adolescence overtly named line drawings corresponding to positive, negative, and neutral concrete nouns. The results of regression and mixed model analyses indicated that positive emotion-laden words are learnt earlier in life. This effect was independent of the contribution of other lexical and semantic factors (familiarity, word frequency, concreteness, word length). It is proposed that the prioritized acquisition of positive emotion-laden words might be the consequence of the communicative style and contextual factors associated with the interaction between children and caregivers. We also discuss the implications of our findings for proposals that highlight the role of language in emotion perception and understanding.
Mapping of gold mineralization using an integrated interpretation of geological and geophysical data—a case study from West Baranes, South Eastern Desert, Egypt
Authors: Gobashy Mohamed M. , Abbas Ehdaa Allah Sayed , Soliman Khalid S. , Abdelhalim Ahmed
Published: November 2022

The current study is mainly devoted to the integration, analysis, and interpretation of the available geologic, remote sensing, and potential field data (mainly magnetic) to delineate the subsurface geologic structural elements controlling the western part of Baranes, South Eastern Desert, Egypt, and its relation to the southwestern desert structural regime. Additionally, to detect potential mineralization zones for future mining plans in the study area. The Western part of Baranes has not had enough geomorphological mapping, consequently, potential discoveries of mineralization zones are very low. So, in this paper, an attempt is initiated to better understand the evolution of the geomorphology and structural regime of the region and construct a digital geological map and structural patterns showing the possible locations of mineralization zones based on the previous knowledge from similar potential sites and focus on the future economic importance of the region. To achieve this purpose, processed Landsat-8 images successfully revealed the lithological contacts and fault zones helping in distinguishing between the different rock units; moreover, the aeromagnetic data available in the area is used and several filters are applied including reduction to the pole, Euler homogeneity equation, analytic Signal (AS), and advanced grid filtering are sequentially used aiming to detect the possible subsurface distribution of mineralization zones from the integrated interpretation of magnetic susceptibilities and available geologic and remote sensing data.
Cucurbit production systems in Australia and biosecurity risks associated with viral pathogens
Authors: Mulholland S.
Published: November 2022

Viral infection is a key limiting factor to cucurbit production globally. The viral population within Australia differs from that elsewhere in the world in that it is represented by relatively few key pathogens from a small number of virus families. Viral infections induce a range of physical deformities and discoloration on leaves and fruit of cucurbit commodities. Typically, viral infections result in the decline of marketable yield and even plant death in extreme cases. Identifying key exotic viral issues and the analysis of existing preparedness plans, surveillance programs and diagnostic options are critical biosecurity strategies required to mitigate the introduction of new pathogens into Australia. This review discusses the biosecurity threat exotic viruses pose to Australian cucurbit production systems and the inherent difficulties associated with managing viral pathogens.
NOTES FROM ROME 2021–22
Authors: Siwicki Christopher
Published: November 2022

This gazette presents to the reader outside Rome news of recent archaeological activity (August 2021 – June 2022) gleaned from public lectures, conferences, exhibitions, and newspaper reports.
Older adults' adaptations to life events: a mobility perspective
Authors: Lowe Thomas A. , de Haas Billie , Osborne Tess , Meijering Louise
Published: November 2022

Mobility research tends to focus on physical movement and experiences in later life; however, changes in older adult mobility over time remains underexplored. Furthermore, older adults typically experience many life events, some of which result from cognitive and physical decline, and many of which impact mobility. This article aims to explore how life events affect the mobility of older adults over time. We conducted in-depth interviews with 22 older adults aged 55 years and over from Lancashire, United Kingdom. Of these participants, eight lived with memory problems. The findings show that both anticipated and unexpected life events play a profound role in the participants' mobility over time. Retirement, long-term illness and age-related illness were examples of anticipated life events, while the death of a loved one and developing memory problems were examples of unexpected life events. In both cases, participants' made external adaptations, such as moving home, or internal adaptations, such as self-awareness. The findings also emphasise the layered nature of life events and adaptations playing a role in the participants' mobility. Additionally, life events such as developing memory problems showed a domino effect, triggering further life events and adaptations which impacted the participants' mobility. This article emphasises how transition periods can occur before or after a life event, showing that adaptations can be pre-emptive to a life event. Our article contributes to calls for internal adaptations to be fully incorporated into age-related policy and also for age-related policy to be more inclusive for older adults who experience memory problems and dementia.
Early results of Pulsta® transcatheter heart valve in patients with enlarged right ventricular outflow tract and severe pulmonary regurgitation due to transannular patch
Authors: Odemis Ender , Yenidogan Irem , Kizilkaya Mete Han
Published: November 2022

Objective:

The purpose of this study is to assess the feasibility, effectivity, and safety of a novel self-expandable valve system, Pulsta® transcatheter heart valve in patients with tetralogy of fallot and severe pulmonary regurgitation after transannular patch repair.

Background:

Severe pulmonary regurgitation after tetralogy of fallot repair is a life-threatening problem and should be treated by pulmonary valve implantation. Although percutaneous pulmonary valve implantation has been ever increasingly used for this purpose, available balloon-expandable valves have limitations and cannot be used by most patients. Pulsta® transcatheter heart valve is a new self-expandable valve system and offers a new solution to be used in patients with different types of native right ventricular outflow tract geometry.

Patients and Methods:

Ten patients with severe regurgitation after tetralogy of fallot repair with a transannular patch have been enrolled in the study according to echocardiographic examination. MRI was used in asymptomatic patients to delineate the indication and the right ventricular outflow tract geometry. Pulsta® transcatheter heart valve implantation was performed in ten patients, and preprocedural, procedure, and 6 months follow-up findings of the patients were evaluated.

Results:

Pulsta® pulmonary valve implantation was performed in ten patients successfully without any severe complications. Valve functions were perfect in six of ten patients, while the others had insignificant regurgitation by echocardiographic examination at the end of 6 months follow-up.

Conclusions:

This study showed that Pulsta® transcatheter heart valve is a feasible, effective, and safe method in the treatment of severe pulmonary regurgitation due to transannular patch repair in patients with tetralogy of fallot.
Article 12 of the Protocol on the Rights of Persons with Disabilities in Africa: A critical analysis
Authors: Tiwa Fomekong Steve
Published: November 2022

This contribution analyses Article 12 of the Protocol to the African Charter on Human and Peoples’ Rights on the Rights of Persons with Disabilities in Africa (the Protocol). It examines the purpose, scope and contribution of this Article to the legal protection of persons with disabilities in armed conflict and its implementation. The analysis is divided into four parts. The first part will start by identifying and analysing the background to this provision, which provides specific protection to persons with disabilities in armed conflict. The second part will examine Article 12 in the light of other similar regional instruments and of the protection challenges that persons with disabilities face during conflict. This will highlight the specific nature of the Article's provisions, together with its shortcomings and its progressive aspects. Part three will look at the interaction between Article 12 and equivalent rules of international humanitarian law, and how Article 12 contributes to the development of legal protection for persons with disabilities in armed conflict. Finally, the fourth part will examine the challenges to the implementation of Article 12. It will also propose ways of overcoming those challenges and hence of enabling Article 12 to have its intended effect.
A Mutual Information Theory‐Based Approach for Assessing Uncertainties in Deterministic Multi‐Category Precipitation Forecasts
Authors: Ning Yawei , Liang Guohua , Ding Wei , Shi Xiaogang , Fan Yurui , Chang Jianxia , Wang Yimin , He Bin , Zhou Huicheng
Published: November 2022

The very nature of weather forecasts and verifications and the way they are used make it impossible for one single or absolute standard of evaluation. However, little research has been conducted on verifying deterministic multi‐category forecasts, which is based on the attribute of uncertainty. The authors propose a new approach using two mutual information theory‐based scores for assessing the comprehensive uncertainty of all categories and the uncertainty for a certain category in deterministic multi‐category precipitation forecasts, respectively. Specifically, the comprehensive uncertainty is defined as the average reduction in uncertainty about the observations resulting from the use of a predictive model to provide all categories forecasts; the uncertainty of a certain category is defined as the reduction in uncertainty about the observations resulting from the use of a predictive model to provide a certain category forecast. By applying the proposed approach and traditional verification methods, the four precipitation forecasting products from the China Meteorological Administration, European Centre for Medium‐Range Weather Forecasts, National Centers for Environmental Prediction, and United Kingdom Meteorological Office were verified in the Dahuofang Reservoir Drainage Basin, China. The results indicate that: (a) the proposed approach can better capture the changing patterns of uncertainties with lead times and distinguish the forecasting performance among different forecast products; (b) the proposed approach is resistant to the extreme bias; (c) the proposed approach needs a careful choice of bin width; and (d) the bias analysis is necessary before verifying the uncertainties in precipitation forecasts.
Simultaneous treatment with sorafenib and glucose restriction inhibits hepatocellular carcinoma in vitro and in vivo by impairing SIAH1-mediated mitophagy
Authors: Zhou Jing , Feng Ji , Wu Yong , Dai Hui-Qi , Zhu Guang-Zhi , Chen Pan-Hong , Wang Li-Ming , Lu Guang , Liao Xi-Wen , Lu Pei-Zhi , Su Wen-Jing , Hooi Shing Chuan , Ye Xin-Pin , Shen Han-Ming , Peng Tao , Lu Guo-Dong
Published: November 2022

Transarterial chemoembolization (TACE) is the first-line treatment for unresectable intermediate-stage hepatocellular carcinoma (HCC). It is of high clinical significance to explore the synergistic effect of TACE with antiangiogenic inhibitors and the molecular mechanisms involved. This study determined that glucose, but not other analyzed nutrients, offered significant protection against cell death induced by sorafenib, as indicated by glucose deprivation sensitizing cells to sorafenib-induced cell death. Next, this synergistic effect was found to be specific to sorafenib, not to lenvatinib or the chemotherapeutic drugs cisplatin and doxorubicin. Mechanistically, sorafenib-induced mitophagy, as indicated by PINK1 accumulation, increased the phospho-poly-ubiquitination modification, accelerated mitochondrial membrane protein and mitochondrial DNA degradation, and increased the amount of mitochondrion-localized mKeima-Red engulfed by lysosomes. Among several E3 ubiquitin ligases tested, SIAH1 was found to be essential for inducing mitophagy; that is, SIAH1 silencing markedly repressed mitophagy and sensitized cells to sorafenib-induced death. Notably, the combined treatment of glucose restriction and sorafenib abolished ATP generation and mitophagy, which led to a high cell death rate. Oligomycin and antimycin, inhibitors of electron transport chain complexes, mimicked the synergistic effect of sorafenib with glucose restriction to promote cell death mediated via mitophagy inhibition. Finally, inhibition of the glucose transporter by canagliflozin (a clinically available drug used for type-II diabetes) effectively synergized with sorafenib to induce HCC cell death in vitro and to inhibit xenograft tumor growth in vivo. This study demonstrates that simultaneous treatment with sorafenib and glucose restriction is an effective approach to treat HCC, suggesting a promising combination strategy such as transarterial sorafenib-embolization (TASE) for the treatment of unresectable HCC.
Rare tandem repeat expansions associate with genes involved in synaptic and neuronal signaling functions in schizophrenia
Authors: Wen Jia , Trost Brett , Engchuan Worrawat , Halvorsen Matthew , Pallotto Linda M. , Mitina Aleksandra , Ancalade NaEshia , Farrell Martilias , Backstrom Ian , Guo Keyi , Pellecchia Giovanna , Thiruvahindrapuram Bhooma , Giusti-Rodriguez Paola , Rosen Jonathan David , Li Yun , Won Hyejung , Magnusson Patrik K. E. , Gyllensten Ulf , Bassett Anne S. , Hultman Christina M. , Sullivan Patrick F. , Yuen Ryan K. C. , Szatkiewicz Jin P.
Published: November 2022

Tandem repeat expansions (TREs) are associated with over 60 monogenic disorders and have recently been implicated in complex disorders such as cancer and autism spectrum disorder. The role of TREs in schizophrenia is now emerging. In this study, we have performed a genome-wide investigation of TREs in schizophrenia. Using genome sequence data from 1154 Swedish schizophrenia cases and 934 ancestry-matched population controls, we have detected genome-wide rare (<0.1% population frequency) TREs that have motifs with a length of 2–20 base pairs. We find that the proportion of individuals carrying rare TREs is significantly higher in the schizophrenia group. There is a significantly higher burden of rare TREs in schizophrenia cases than in controls in genic regions, particularly in postsynaptic genes, in genes overlapping brain expression quantitative trait loci, and in brain-expressed genes that are differentially expressed between schizophrenia cases and controls. We demonstrate that TRE-associated genes are more constrained and primarily impact synaptic and neuronal signaling functions. These results have been replicated in an independent Canadian sample that consisted of 252 schizophrenia cases of European ancestry and 222 ancestry-matched controls. Our results support the involvement of rare TREs in schizophrenia etiology.
War exposure, post-traumatic stress symptoms and hair cortisol concentrations in Syrian refugee children
Authors: Smeeth Demelza , McEwen Fiona S. , Popham Cassandra M. , Karam Elie G. , Fayyad John , Saab Dahlia , Rieder Michael J. , Elzagallaai Abdelbaset A. , van Uum Stan , Pluess Michael
Published: November 2022

Altered secretion of cortisol, the primary effector of the hypothalamus–pituitary–adrenal axis, has been proposed as a means by which traumatic experiences compromise later mental health. However, despite the popularity of cortisol as a potential biomarker for stress and adversity, findings are inconsistent, and little is known about the impact of war-related trauma on stress physiology of children and adolescents. Here we aimed to evaluate the relationships between war exposure, current living conditions, hair cortisol concentrations (HCC) and post-traumatic stress disorder (PTSD) symptoms in a large cohort of Syrian refugee children and adolescents (6–18 years) and their caregiver. This longitudinal observational study assessed Syrian refugee children and adolescents in two waves, 1 year apart, within informal tented settlements in Lebanon. The relationships between war exposure, time since leaving Syria, PTSD symptoms and HCC were investigated using linear mixed-model regression utilising both waves of data collected (Y1: N = 1574, Y2: N = 923). Hair cortisol concentration was positively, but weakly associated with the number of war-related events experienced. This was limited to those who were at least 12 years old at the time of war exposure. Conversely, HCC decreased with time since leaving Syria. HCC was also associated with PTSD symptoms but not with the quality of their current living conditions. This study revealed that changes to hypothalamic-pituitary-adrenal axis activity may accompany both earlier war exposure and current PTSD symptoms in children and adolescents. Additionally, early adolescence may be a particularly sensitive time in terms of trauma-related changes to the hypothalamic-pituitary-adrenal axis.
Does cannabidiol make cannabis safer? A randomised, double-blind, cross-over trial of cannabis with four different CBD:THC ratios
Authors: Englund Amir , Oliver Dominic , Chesney Edward , Chester Lucy , Wilson Jack , Sovi Simina , De Micheli Andrea , Hodsoll John , Fusar-Poli Paolo , Strang John , Murray Robin M. , Freeman Tom P. , McGuire Philip
Published: November 2022

As countries adopt more permissive cannabis policies, it is increasingly important to identify strategies that can reduce the harmful effects of cannabis use. This study aimed to determine if increasing the CBD content of cannabis can reduce its harmful effects. Forty-six healthy, infrequent cannabis users participated in a double-blind, within-subject, randomised trial of cannabis preparations varying in CBD content. There was an initial baseline visit followed by four drug administration visits, in which participants inhaled vaporised cannabis containing 10 mg THC and either 0 mg (0:1 CBD:THC), 10 mg (1:1), 20 mg (2:1), or 30 mg (3:1) CBD, in a randomised, counter-balanced order. The primary outcome was change in delayed verbal recall on the Hopkins Verbal Learning Task. Secondary outcomes included change in severity of psychotic symptoms (e.g., Positive and Negative Syndrome Scale [PANSS] positive subscale), plus further cognitive, subjective, pleasurable, pharmacological and physiological effects. Serial plasma concentrations of THC and CBD were measured. THC (0:1) was associated with impaired delayed verbal recall (t(45) = 3.399, d = 0.50, p = 0.001) and induced positive psychotic symptoms on the PANSS (t(45) = −4.709, d = 0.69, p = 2.41 × 10–5). These effects were not significantly modulated by any dose of CBD. Furthermore, there was no evidence of CBD modulating the effects of THC on other cognitive, psychotic, subjective, pleasurable, and physiological measures. There was a dose-response relationship between CBD dose and plasma CBD concentration, with no effect on plasma THC concentrations. At CBD:THC ratios most common in medicinal and recreational cannabis products, we found no evidence that CBD protects against the acute adverse effects of cannabis. This should be considered in health policy and safety decisions about medicinal and recreational cannabis.
KCNF1 promotes lung cancer by modulating ITGB4 expression
Authors: Chen Ching-Yi , Wu Pei-Ying , Van Scoyk Michelle , Simko Stephanie A. , Chou Chu-Fang , Winn Robert A.
Published: November 2022

Lung cancer continues to be the leading cause of cancer death in the United States. Despite recent advances, the five-year survival rate for lung cancer compared to other cancers still remains fairly low. The discovery of molecular targets for lung cancer is key to the development of new approaches and therapies. Electrically silent voltage-gated potassium channel (KvS) subfamilies, which are unable to form functional homotetramers, are implicated in cell-cycle progression, cell proliferation and tumorigenesis. Here, we analyzed the expression of KvS subfamilies in human lung tumors and identified that potassium voltage-gated channel subfamily F member 1 (KCNF1) was up-regulated in non-small cell lung cancer (NSCLC). Silencing of KCNF1 in NSCLC cell lines reduced cell proliferation and tumor progression in mouse xenografts, re-established the integrity of the basement membrane, and enhanced cisplatin sensitivity. KCNF1 was predominately localized in the nucleoplasm and likely mediated its functions in an ion-independent manner. We identified integrin β4 subunit (ITGB4) as a downstream target for KCNF1. Our findings suggest that KCNF1 promotes lung cancer by enhancing ITGB4 signaling and implicate KCNF1 as a novel therapeutic target for lung cancer.
Effect of the intratumoral microbiota on spatial and cellular heterogeneity in cancer
Authors: Galeano Niño Jorge Luis , Wu Hanrui , LaCourse Kaitlyn D. , Kempchinsky Andrew G. , Baryiames Alexander , Barber Brittany , Futran Neal , Houlton Jeffrey , Sather Cassie , Sicinska Ewa , Taylor Alison , Minot Samuel S. , Johnston Christopher D. , Bullman Susan
Published: November 2022

The tumour-associated microbiota is an intrinsic component of the tumour microenvironment across human cancer types1,2. Intratumoral host–microbiota studies have so far largely relied on bulk tissue analysis1–3, which obscures the spatial distribution and localized effect of the microbiota within tumours. Here, by applying in situ spatial-profiling technologies4 and single-cell RNA sequencing5 to oral squamous cell carcinoma and colorectal cancer, we reveal spatial, cellular and molecular host–microbe interactions. We adapted 10x Visium spatial transcriptomics to determine the identity and in situ location of intratumoral microbial communities within patient tissues. Using GeoMx digital spatial profiling6, we show that bacterial communities populate microniches that are less vascularized, highly immuno‑suppressive and associated with malignant cells with lower levels of Ki-67 as compared to bacteria-negative tumour regions. We developed a single-cell RNA-sequencing method that we name INVADEseq (invasion–adhesion-directed expression sequencing) and, by applying this to patient tumours, identify cell-associated bacteria and the host cells with which they interact, as well as uncovering alterations in transcriptional pathways that are involved in inflammation, metastasis, cell dormancy and DNA repair. Through functional studies, we show that cancer cells that are infected with bacteria invade their surrounding environment as single cells and recruit myeloid cells to bacterial regions. Collectively, our data reveal that the distribution of the microbiota within a tumour is not random; instead, it is highly organized in microniches with immune and epithelial cell functions that promote cancer progression.
Arbuscular mycorrhiza alters the nutritional requirements in Salvia miltiorrhiza and low nitrogen enhances the mycorrhizal efficiency
Authors: Pu Chunjuan , Yang Guang , Li Pengying , Ge Yang , Garran Thomas Avery , Zhou Xiuteng , Shen Ye , Zheng Han , Chen Meilan , Huang Luqi
Published: November 2022

Salvia miltiorrhiza Bunge (danshen in Chinese) is one of the most important medicinal cash crops in China. Previously, we showed that arbuscular mycorrhizal fungi (AMF) can promote S. miltiorrhiza growth and the accumulation of bioactive compounds. Fertilization may affect mycorrhizal efficiency, and appropriate doses of phosphate (P) and nitrogen (N) fertilizers are key factors for obtaining mycorrhizal benefits. However, the optimal fertilization amount for mycorrhizal S. miltiorrhiza remains unclear. In this study, we studied the effects of AMF on the growth and bioactive compounds of S. miltiorrhiza under different doses (low, medium, and high) of P and N fertilizer. The results showed that the mycorrhizal growth response (MGR) and mycorrhizal response of bioactive compounds (MBC) decreased gradually with increasing P addition. Application of a low (N25) dose of N fertilizer significantly increased the MGR of mycorrhizal S. miltiorrhiza, and a medium (N50) dose of N fertilizer significantly increased the MBC of phenolic acids, but decreased the MBC of tanshinones. Our results also showed that the existence of arbuscular mycorrhiza changes nutrient requirement pattern of S. miltiorrhiza. P is the limiting nutrient of non-mycorrhizal plants whereas N is the limiting nutrient of mycorrhizal plants.
Working memory capacity estimates moderate value learning for outcome-irrelevant features
Authors: Ben-Artzi Ido , Luria Roy , Shahar Nitzan
Published: November 2022

To establish accurate action-outcome associations in the environment, individuals must refrain from assigning value to outcome-irrelevant features. However, studies have largely ignored the role of attentional control processes on action value updating. In the current study, we examined the extent to which working memory—a system that can filter and block the processing of irrelevant information in one’s mind—also filters outcome-irrelevant information during value-based learning. For this aim, 174 individuals completed a well-established working memory capacity measurement and a reinforcement learning task designed to estimate outcome-irrelevant learning. We replicated previous studies showing a group-level tendency to assign value to tasks’ response keys, despite clear instructions and practice suggesting they are irrelevant to the prediction of monetary outcomes. Importantly, individuals with higher working memory capacity were less likely to assign value to the outcome-irrelevant response keys, thus suggesting a significant moderation effect of working memory capacity on outcome-irrelevant learning. We discuss the role of working memory processing on value-based learning through the lens of a cognitive control failure.
Personalized prediction of optimal water intake in adult population by blended use of machine learning and clinical data
Authors: Dolci Alberto , Vanhaecke Tiphaine , Qiu Jiqiong , Ceccato Riccardo , Arboretti Rosa , Salmaso Luigi
Published: November 2022

Growing evidence suggests that sustained concentrated urine contributes to chronic metabolic and kidney diseases. Recent results indicate that a daily urinary concentration of 500 mOsm/kg reflects optimal hydration. This study aims at providing personalized advice for daily water intake considering personal intrinsic (age, sex, height, weight) and extrinsic (food and fluid intakes) characteristics to achieve a target urine osmolality (UOsm) of 500 mOsm/kg using machine learning and optimization algorithms. Data from clinical trials on hydration (four randomized and three non-randomized trials) were analyzed. Several machine learning methods were tested to predict UOsm. The predictive performance of the developed algorithm was evaluated against current dietary guidelines. Features linked to urine production and fluid consumption were listed among the most important features with relative importance values ranging from 0.10 to 0.95. XGBoost appeared the most performing approach (Mean Absolute Error (MAE) = 124.99) to predict UOsm. The developed algorithm exhibited the highest overall correct classification rate (85.5%) versus that of dietary guidelines (77.8%). This machine learning application provides personalized advice for daily water intake to achieve optimal hydration and may be considered as a primary prevention tool to counteract the increased incidence of chronic metabolic and kidney diseases.
Motion estimation for large displacements and deformations
Authors: Chen Qiao , Poullis Charalambos
Published: November 2022

Large displacement optical flow is an integral part of many computer vision tasks. Variational optical flow techniques based on a coarse-to-fine scheme interpolate sparse matches and locally optimize an energy model conditioned on colour, gradient and smoothness, making them sensitive to noise in the sparse matches, deformations, and arbitrarily large displacements. This paper addresses this problem and presents HybridFlow, a variational motion estimation framework for large displacements and deformations. A multi-scale hybrid matching approach is performed on the image pairs. Coarse-scale clusters formed by classifying pixels according to their feature descriptors are matched using the clusters’ context descriptors. We apply a multi-scale graph matching on the finer-scale superpixels contained within each matched pair of coarse-scale clusters. Small clusters that cannot be further subdivided are matched using localized feature matching. Together, these initial matches form the flow, which is propagated by an edge-preserving interpolation and variational refinement. Our approach does not require training and is robust to substantial displacements and rigid and non-rigid transformations due to motion in the scene, making it ideal for large-scale imagery such as aerial imagery. More notably, HybridFlow works on directed graphs of arbitrary topology representing perceptual groups, which improves motion estimation in the presence of significant deformations. We demonstrate HybridFlow’s superior performance to state-of-the-art variational techniques on two benchmark datasets and report comparable results with state-of-the-art deep-learning-based techniques.
Impact of chitosan administration on titanium dioxide nanoparticles induced testicular dysfunction
Authors: Halawa Amal A. , Elshopakey Gehad E. , Elmetwally Mohammed A. , El-Adl Mohamed , Lashen Samah , Shalaby Nancy , Eldomany Ehab , Farghali Ahmed , Sayed-Ahmed Mohamed Z. , Alam Nawazish , Syed Nabeel Kashan , Ahmad Sarfaraz , Rezk Shaymaa
Published: November 2022

The potential reproductive toxic effects of oral TiO2 NPs in adult male rats as well as the possible alleviation of chitosan administration was investigated. Animals were allocated to four groups; the first group received deionized water and was assigned as a control group. In the second group, rats received chitosan at a dose of 5 mg/kg BW/day. The third group was designed for administration of TiO2 NPs at a dose of 150 mg/kg BW/day (1/80 LD50). Rats in the fourth group received both TiO2 NPs and chitosan. After 14 days, TiO2 NPs induced testicular lipid peroxidation as well as oxidative stress. Nano-titanium significantly upregulated genes that encode apoptosis and inflammation in testicular tissue. Moreover, it induced histological alteration in the testicular structure with impairment in spermatogenesis via reduction of PCNA immune-staining. Chitosan administration significantly improved the activities of testicular GPx, SOD, and CAT enzymes. In addition, it significantly down-regulated the relative expressions of pro-apoptotic and pro-inflammatory testicular genes. Chitosan was able to improve the testicular architecture as well as spermatogenesis. The current study revealed the capability of chitosan to ameliorate nano-titanium induced testicular toxicity. Thus, attention should be given to the extensive consumption of nano-titanium particles.
Singlet fission initiating triplet generations of BODIPY derivatives through $$\pi $$-stacking: a theoretical study
Authors: Tsuneda Takao , Taketsugu Tetsuya
Published: November 2022

The role of singlet fission (SF) in the triplet-state generation mechanism of 1,3,5,7-tetramethyl-boron-dipyrromethene derivatives is revealed by exploring the cause for the solvent dependence of the generation rate. Comparing the adsorption energy calculations of solvent molecules, i.e., cyclohexane, chloroform and acetonitrile molecules, to the derivatives with the $$\pi $$
π
-stacking energies of these derivatives surprisingly show that the hierarchy of the solvation energies and $$\pi $$
π
-stacking energies strongly correlates with the experimentally-suggested solvent dependence of the triplet-state generation of these derivatives for five and more adsorbing solvent molecules. Following this finding, the excitation spectra of these derivatives in acetonitrile solvent are explored using the proprietary spin-flip long-range corrected time-dependent density functional theory. It is, consequently, confirmed that the $$\pi $$
π
-stacking activates the second lowest singlet excitation to trigger the spin-allowed transition to the singlet doubly-excited tetraradical (TT)$$_1$$

1

state, which generates the long-lived quintet (TT)$$_1$$

1

state causing the SF. However, it is also found that the $$\pi $$
π
-stacking also get a slow intersystem crossing active around the intersections of the lowest singlet excitations with the lowest triplet T$$_1$$

1

excitations in parallel with the SF due to the charge transfer characters of the lowest singlet excitations. These results suggest that SF initiates the triplet-state generations through near-degenerate low-lying singlet and (TT) excitations with a considerable singlet-triplet energy gap after the $$\pi $$
π
-stacking of chromophores stronger than but not far from the solvation. Since these derivatives are organic photosensitizers, this study proposes that SF should be taken into consideration in developing novel heavy atom-free organic photosensitizers, which will contribute to a variety of research fields such as medical care, photobiology, energy science, and synthetic chemistry.
Carer distress among community living older adults with complex needs in the pre- and post-COVID-19 era: a national population study
Authors: Schluter Philip J. , Abey-Nesbit Rebecca , Ahuriri-Driscoll Annabel , Bergler Hans Ulrich , Broadbent Jacqueline C. , Glanville Michaela , Keeling Sally , Jamieson Hamish A.
Published: November 2022

Carer distress is one important negative impact of caregiving and likely exacerbated by the novel coronavirus disease 2019 (COVID-19) pandemic, yet little population-based epidemiological information exists. Using national data from repeated standardized comprehensive geriatric needs assessments, this study aims to: describe the pattern of caregiver distress among those providing informal care to community-living adults aged ≥ 65 years with complex needs in New Zealand over time; estimate the COVID-19 effect on this temporal pattern; and, investigate relationships between participants’ sociodemographic and selected health measures on caregiver distress. Fractional polynomial regression and multivariable multilevel mixed-effects models were employed. Overall, 231,277 assessments from 144,358 participants were analysed. At first assessment, average age was 82.0 years (range 65–107 years), and 85,676 (59.4%) were female. Carer distress prevalence increased from 35.1% on 5 July 2012 to a peak of 48.5% on 21 March 2020, when the New Zealand Government announced a national lock-down. However, the population attributional fraction associated with the COVID-19 period was small, estimated at 0.56% (95% CI 0.35%, 0.77%). Carer distress is common and has rapidly increased in recent years. While significant, the COVID-19 impact has been relatively small. Policies and services providing efficacious on-going strategies to support caregivers deserves specific attention.
Limited transfer and retention of locomotor adaptations from virtual reality obstacle avoidance to the physical world
Authors: Weber Anika , Hartmann Ulrich , Werth Julian , Epro Gaspar , Seeley John , Nickel Peter , Karamanidis Kiros
Published: November 2022

Locomotor training based in virtual reality (VR) is promising for motor skill learning, with transfer of VR skills in turn required to benefit daily life locomotion. This study aimed to assess whether VR-adapted obstacle avoidance can be transferred to a physical obstacle and whether such transfer is retained after 1 week. Thirty-two young adults were randomly divided between two groups. A control group (CG) merely walked on a treadmill and an intervention group (IG) trained crossing 50 suddenly-appearing virtual obstacles. Both groups crossed three physical obstacles (transfer task) immediately after training (T1) and 1 week later (T2, transfer retention). Repeated practice in VR led to a decrease in toe clearance along with greater ankle plantarflexion and knee extension. IG participants crossed physical obstacles with a lower toe clearance compared to CG but revealed significantly higher values compared to the VR condition. VR adaptation was fully retained over 1 week. For physical obstacle avoidance there were differences between toe clearance of the third obstacle at T1 and the first obstacle at T2, indicating only partial transfer retention. We suggest that perception–action coupling, and thus sensorimotor coordination, may differ between VR and the physical world, potentially limiting retained transfer between conditions.
First-principles investigation of polytypic defects in InP
Authors: Vedel Christian Dam , Smidstrup Søren , Georgiev Vihar P.
Published: November 2022

In this paper we study polytypic defects in Indium Phosphide (InP) using the complementary first-principles methods of density functional theory and non-equilibrium Greens functions. Specifically we study interfaces between the ground state Zincblende crystal structure and the meta-stable Wurtzite phase, with an emphasis on the rotational twin plane defect, which forms due to the polytypic nature of InP. We found that the transition of the band structure across the interface is anisotropic and lasts 7 nm (3.5 nm). Due to this, a crystal-phase quantum well would require a minimal width of 10 nm, which eliminates rotational twin planes as possible quantum wells. We also found that for conducting current, the interfaces increase conductivity along the defect-plane ([11$$\bar{2}$$

2

¯

]), whereas due to real growth limitations, despite the interfaces reducing conductivity across the defect-plane ([111]), we found that a high degree of polytypic defects are still desirable. This was argued to be the case, due to a higher fraction of Wurtzite segments in a highly phase-intermixed system.