bluuebunny/crossref_metadata_2025 huggingface dataset:
Disenchanted With the Immigrant Dream: The Sociological Formation of Ex-Immigrant Subjectivity
Authors: Richard Thomas Jacob
Published: March 2024

Although many return migrants had planned to go back to their country of origin, other return migrants who had originally planned to pursue the immigrant dream––the idea that one can have a better life by migrating—eventually change their mind and return to their society of origin. Just as the identity of individuals must adjust to the loss of a relationship, job, or membership of a group, many formerly aspiring immigrants give up on immigrating. I analyze data from oral history interviews and social media narratives of 121 Chinese ex-immigrants from 16 different countries. I show how disappointments, the language barrier, cultural alienation, racial-ethnic discrimination, intersectional-gender issues, barriers to upward socioeconomic mobility, and/or restrictive immigration policies interact with each other to constitute ex-immigrant subjectivity. This article highlights why immigration is a far less certain process than many perceive it to be, contributing to the broader sociological literature on how in different and similar ways processes of abandoning important social endeavors, like a romantic partnership, political party, or religious faith, transform subjectivities and social identities in other domains of social life.
Nutrition and public health in Georgia: reviewing the current status and inspiring improvements: a joint event of the Georgian Nutrition Society, The Nutrition Society of the UK and Ireland and the Sabri Ülker Foundation, October 2023
Authors: Stowell Julian D. , Mathers John C. , Stanley Manana , Baran Aksakal F. Nur , Aslett-Bentley Avril , Bánáti Diána , Bobokhidze Eka , Üliç Çatar Özlem , Dinç Özge , Gvamichava Rusudan , Mutus Begüm , Saunders Caroline , Shannon Oliver Michael , Tewfik Ihab
Published: March 2024

Georgia lies to the northeast of Türkiye, having a western border on the Black Sea. With a population of some 3·73 million, Georgia has a tradition of gastronomic excellence dating back millennia. However, changing lifestyles and external influences have, as elsewhere, led to problems of suboptimal nutrition, and lifestyle-related diseases and disorders prevail. There is considerable scope for improving the focus on public health (PH) and nutrition in Georgia. With this in mind, the Georgian Nutrition Society teamed up with The Nutrition Society of the UK and Ireland and the Sabri Ülker Foundation, a PH charity based in Istanbul, Türkiye, to host a conference and workshops in Tbilisi, Georgia. The primary purpose was to review the current status of PH and nutrition in Georgia with reference to the situation elsewhere, to share examples of best practice and to identify opportunities for improvement. A particular highlight was the presentation of a programme of nutrition education for family physicians recently implemented in Türkiye. This summary of the proceedings is intended as a blueprint for action in Georgia and also to inspire others to consider how PH might be improved via a focus on balanced nutrition.
Unbounded Sturm attractors for quasilinear parabolic equations
Authors: Lappicy Phillipo , Fernandes Juliana
Published: March 2024

We analyse the asymptotic dynamics of quasilinear parabolic equations when solutions may grow up (i.e. blow up in infinite time). For such models, there is a global attractor which is unbounded and the semiflow induces a nonlinear dynamics at infinity by means of a Poincaré projection. In case the dynamics at infinity is given by a semilinear equation, then it is gradient, consisting of the so-called equilibria at infinity and their corresponding heteroclinics. Moreover, the diffusion and reaction compete for the dimensionality of the induced dynamics at infinity. If the equilibria are hyperbolic, we explicitly prove the occurrence of heteroclinics between bounded equilibria and/or equilibria at infinity. These unbounded global attractors describe the space of admissible initial data at event horizons of certain black holes.
Plant growth regulators improve root growth of rice seedlings after mechanical transplanting and increase grain yield
Authors: Tang Jichao , Zhang Zhimin , Tung Shahbaz Atta , Lu Bilin , Yang Wenjia
Published: March 2024

Enhancing seedling quality and promoting root growth post-transplantation are crucial for improving mechanically transplanted rice productivity. Here we investigated the impact of various plant growth regulators on hybrid and conventional rice varieties. Treatments, including two-diethylaminoethyl hexanoate (DA-6, 10 mg L−1), a combination of potassium 3-indole-butyrate + potassium 1-naphthylacetate + 6-benzylaminopurine (C3, 50 + 50 + 10 mg L−1), potassium 3-indole-butyrate + potassium 1-naphthylacetate + 1-triacontanol (C4, 50 + 50 + 50 mg L−1), potassium 3-indole-butyrate + potassium 1-naphthylacetate + 1.8% sodium nitrophenolate (C5, 50 + 50 + 1 mg L−1), and a combination of potassium 3-indole-butyrate + potassium 1-naphthylacetate + 1.8% sodium nitrophenolate + DA-6 (C6, 50 + 50 + 1 + 10 mg L−1), were sprayed either 3 or 10 days before transplanting. Seedlings sprayed 10 days before transplanting exhibited a higher number of white roots and total roots at the returning green stage, along with increased grain yield, irrespective of the plant growth regulator used. The C6 combination emerged as the most effective treatment, enhancing the growth of both hybrid and conventional rice seedlings, accelerating the growth rate of white roots and total roots, and increasing the length of the longest white root during the greening period, ultimately resulting in higher grain yield. Our findings demonstrate that pre-transplantation application of a combination of plant growth regulators positively influences rice seedling growth.
Provision of bone conduction hearing implants in England in adults and children: a review of Hospital Episode Statistics data 2012–2021
Authors: Conybeare Alison , Bennett Lauren , Osborne Max S
Published: March 2024

Objective

Bone conduction hearing implants are a well-established method of hearing rehabilitation in children and adults. This study aimed to review any changes in provision in England.

Methods

The total number of bone conduction hearing implantations performed was analysed from 2012 to 2021 utilising Hospital Episode Statistics data for England.

Results

The total number of procedures has increased by 58 per cent. One-stage bone conduction hearing implantations in adults accounts for the largest proportion of this increase (93 per cent of the total). The number performed in children has remained stable and accounts for 73 per cent (n = 433) of all two-stage procedures.

Conclusion

The data show that bone conduction hearing implant surgery is becoming increasingly popular, particularly in adults. This has correlated with the increase in availability, national recommendations and choice of devices.
Does coronavirus disease 2019 affect peripheral and central auditory systems? Matched group cross-sectional study and six-month follow up
Authors: Deniz-Sakarya Merve , Yorulmaz İrfan
Published: March 2024

Objective

This study aimed to compare the peripheral-to-central auditory systems of people with coronavirus disease 2019 to a well-matched control group and examine the long-term effects of coronavirus disease 2019 on the auditory system.

Method

Participants who were outpatients of coronavirus disease 2019 (n = 30) were compared with a well-matched control group (n = 30). Behavioural and electrophysiological tests were performed, and tests were repeated at six months in the coronavirus disease 2019 group.

Results

Statistically significant differences were observed in the right ear at 10 kHz (p = 0.007) and 12.5 kHz (p = 0.028), and in the left ear at 10 kHz (p = 0.040) and 12.5 kHz (p = 0.040) between groups. The groups had no difference regarding the other audiological test results (p > 0.05).

Conclusion

Extended high-frequency thresholds were affected in the coronavirus disease 2019 patients. No other findings indicated that the peripheral-to-central auditory system was affected. The effect on extended high-frequency thresholds appeared permanent, but no clinically significant new, late-onset auditory system effects were observed.
“Yugoslavia is worthless . . . you can get neither sugar nor kerosene.” Food Supply and Political Legitimacy in the Slovene Part of Yugoslavia, 1918–1924
Authors: Stergar Rok
Published: March 2024

The new states that were established in the autumn of 1918 presented themselves as something new and better. Not only were they supposed to be the embodiment of the “national yearnings” of the formerly “oppressed nations” of the Habsburg Empire, but they were also meant to be more democratic and it was promised that their administrations would work better and their economies would flourish. In short, they were to be a decisive break with the imperial past. However, the new nation-states often could not deliver on these lofty promises, and, as a result, their legitimacy began to erode rather rapidly. In this context, the inability to quickly improve the food supply played an important role. In the Slovene part of Yugoslavia, the inadequate supply of basic foodstuffs, rationing, and increasing prices made the already volatile situation worse, as parts of the population began to grumble, protest, and yearn for the Habsburgs, looking across their northern and western borders. Police and court files, district captains’ reports, and various other sources indicate that after the proclamation of independence the mood of the population quickly soured, and that the legitimacy of the new state was often questioned.
Food Shortages during the Post-Habsburg Transition in the Bohemian Lands and Slovenia
Authors: Šmidrkal Václav , Stergar Rok
Published: March 2024

This article introduces the forum on food shortages during the post-Habsburg transition in the Bohemian Lands and Slovenia. Using examples from these regions, it first outlines the food crisis that developed during World War I and contributed to the internal disintegration of the Habsburg Empire. The article then turns to Czechoslovakia and Yugoslavia, successor states which, despite their victorious status and optimistic prospects for the future, had to contend with food shortages that lasted well beyond 1918. Shortages remained one of the main challenges to the consolidation of these newly formed states. Finally, and most importantly, the article provides an overview of the state of the art in Czech, Slovene, and international historiography, identifies gaps in knowledge, and presents our approach to the topic.
“Who Could Be Strong When Hungry?”: Food Supply and Nutrition of the Civilian Population in Maribor at the End of and after World War I
Authors: Godina Golija Maja
Published: March 2024

The end of World War I brought not only the end of a great slaughter but also the creation of new countries, great expectations of better living conditions, and the promise of an end of scarcity. In Maribor, a contested border town occupied by Slovenian troops and annexed to the newly established State of Slovenes, Croats, and Serbs, expectations were even higher. A part of the population opposed the town's annexation to the newly established state and compared the living conditions at home with those in Austria. As early as November 1918, the Slovene City Food Council was established in Maribor to feed the city's population. It introduced measures similar to those introduced during the war, such as food ration cards. Despite these measures, food shortages and hunger were part of everyday life, especially in the winter of 1918–19. This article discusses civilians' survival strategies, as well as continuities and discontinuities between wartime and postwar measures to improve the food supply. It shows that despite the efforts of the new Yugoslav authorities, they often continued wartime practices and food remained of poor quality and difficult to access for most of the population throughout 1919.
Legitimate governance in international politics: Towards a relational theory of legitimation
Authors: Minatti Wolfgang
Published: March 2024

How do governing actors in international politics become legitimised? Current approaches to the study of legitimation do not fully account for the complexities of governance in contemporary international and global politics because they pre-specify ‘sources’ of legitimacy and treat change in audience expectations towards rightful rule as exogenous to legitimation processes. Instead, this article synthesises existing models of legitimation with relational theory to argue that constellations of institutional complexities necessitate an analytical focus on audiences and their expectations as embedded in governance networks. It then provides a relational theory of legitimation, emphasising the mechanisms undergirding legitimation: legitimation should be conceptualised as a process of congruence-finding between actors’ normative expectations. A governance relation might be influenced towards greater or lesser congruence via several mechanisms working at the level of the relation and the wider network, with more congruence giving rise to stabler governance practices. In this way, the theory builds upon legitimation scholarship by developing pathways to investigate legitimation across the varied contexts of international politics: it avoids a normative background theory of legitimacy sources and provides an improved framework for understanding change in the legitimacy of institutions over time by considering endogenous mechanisms of legitimation.
Are our parents our neighbours? An ubu-ntu perspective on the golden rule with regard to ageing
Authors: Ramose Mogobe
Published: March 2024

Modu wa taba

Legae ke karolo ye botlhokwa ya setshaba. Tabakgolo mo taodishong ye ke go araba potjisho ye: na ke tshwanelo gore bana ba ishe batswadi ba bona kgole kua madulong a batsofe? Re araba potjisho ye ka go ganetja bana ba ba phedilego gabotse basa babjwe go isha batswadi mafelong a botsofe. Re tloga re bontsha le gore kgale-kgale gona mafatsheng a Bodikela gobe go na le motlhalefi bare ke Cicero. Le yena o kwana le kganetjo ye moka le moreti mogolo John Milton. Botee magareng ga bao ba phelago, badimo le bao ba sa tlo belegwa bo a senyega ge bana ba isha batswadi madulong a batsofe.

Taking the African conception of community as our point of departure, we argue that the advancing age of parents is no justification for their children to transfer the responsibility of caring for their parents to others, such as to nursing homes. Usefulness is not the defining feature of the worth of an elderly human being, especially a parent. We will show by appeal to Cicero that this understanding was alive in Western antiquity and was upheld in the modern period by, for example, John Milton the poet. To send one’s parents to a nursing home when one is in good health is to destroy and kill the triangular relationship between parents and children.
The role of lncRNAKCNQ1OT1/miR-301b/Tcf7 axis in cardiac hypertrophy
Authors: E Mingyao , Ren Feifei , Yu Yanhua , Li Haiyan , Shen Chao
Published: March 2024

Objective:

Cardiac hypertrophy, acting as a pathologic process of chronic hypertension and coronary disease, and its underlying mechanisms still need to be explored. Long non-coding RNA (LncRNA) potassium voltage-gated channel subfamily Q member 1 Transcript 1 (KCNQ1OT1) has been implicated in myocardial infarction. However, its role in cardiac hypertrophy remains reported.

Method:

To explore the regulated effect of lncRNAKCNQ1OT1 and miR-301b in cardiac hypertrophy, gain-and-lose function assays were tested. The expression of lncRNAKCNQ1OT1 and miR-301b were tested by quantitative real time polymerase chain reaction (qRT-PCR). The levels of transcription factor 7 (Tcf7), Proto-oncogene c-myc (c-myc), Brainnatriureticpeptide (BNP) and β-myosin heavy chain (β-MHC) were detected by Western blot. Additionally, luciferase analysis revealed interaction between lncRNAKCNQ1OT1, BNPβ-MHCmiR-301b, and Tcf7.

Result:

LncRNAKCNQ1OT1 overexpression significantly induced cardiac hypertrophy. Furthermore, lncRNAKCNQ1OT1 acts as a sponge for microRNA-301b, which exhibited lower expression in cardiac hypertrophy model, indicating an anti-hypertrophic role. Furthermore, the BNP and β-MHC expression increased, as well as cardiomyocyte surface area, with Ang II treatment, while the effect was repealed by miR-301b. Moreover, the protein expression of Tcf7 was inversely regulated by miR-301b and Antisense miRNA oligonucleotides (AMO)-301b.

Conclusion:

Our study has shown that overexpression of lncRNAKCNQ1OT1 could promote the development of cardiac hypertrophy by regulating miR-301b and Tcf7. Therefore, inhibition of lncRNAKCNQ1OT1 might be a potential therapeutic strategy for cardiac hypertrophy.
The Relationship Between Lactate and Lactate Clearance with In-Hospital Mortality in Unselected Emergency Department Patients
Authors: Susur Ozlem , Yesіlaras Murat , Eyler Yesim
Published: March 2024

Introduction:

Lactate is a frequently used biomarker in emergency departments (EDs), especially in critically ill patients. The aim of this study is to investigate the relationship between lactate and lactate clearance with in-hospital mortality in unselected ED patients.

Methods:

This study was carried out retrospectively in the ED of a tertiary hospital. Patients aged 18 years and older whose blood lactate level was obtained in the ED were included in the study. Patients whose lactate value did not have sufficient analytical accuracy, whose lactate value was recorded in the system 180 minutes after admission, who were admitted to the ED as cardiac arrest, and whose ED or hospital outcome was unknown were excluded from the study. According to the first measured lactate value, the patients were divided into three groups: < 2.0mmol/L, 2.0-3.9mmol/L, and ≥ 4.0mmol/L. Lactate clearance was calculated and recorded in patients with one-to-four hours between two lactate values.

Results:

During the five-year study period, a total of 1,070,406 patients were admitted to the ED, of which 114,438 (10.7%) received blood gas analysis. The median age of 81,449 patients included in the study was 58 years (IQR: 30, min: 18–max: 117) and 54.4% were female. The study found that non-trauma patients with a lactate level between 2.0-3.9mmol/L had a 2.5-times higher mortality risk, while those with a lactate level of ≥ 4.0mmol/L had a 20.8-times higher risk, compared to those with a lactate level < 2.0mmol/L. For trauma patients, the mortality risk was three-times higher for those with lactate levels between 2.0-3.9mmol/L and nine-times higher for those with a lactate level of ≥ 4.0mmol/L, compared to those with a lactate level < 2.0mmol/L. Among patients with a first measured lactate value ≥ 4.0mmol/L and a two-hour lactate clearance < 20%, the mortality rate was 19.7%. In addition, lactate, lactate clearance, and age were independent variables for mortality in this patient group.

Conclusion:

The lactate value in unselected patients in the ED is a biomarker that can be used to predict the prognosis of the patients. In addition, lactate, lactate clearance, and age are independent predictors of mortality.
A Serious Shortfall in Clinical Research in Doctoral Schools: A Detailed Analysis of Ten Doctoral Schools of Medicine
Authors: Hegyi Judit , Nagy Rita , Kói Tamás , Hegyi Péter
Published: March 2024

The amount and quality of clinical research are constantly increasing; however, the translation of results into daily practice is not keeping pace. University curricula provide minimal methodological background for understanding the latest scientific findings. In this project, we aimed to investigate the quality and amount of clinical research compared with basic research by analysing ten doctoral schools in Hungary. We found that 71% of PhD theses were submitted in basic sciences. The majority of physicians (53%) working in clinical institutions did their PhD projects in theoretical departments. Importantly, recent clinical methodologies such as pre-registered randomized clinical trials and meta-analysis are only rarely used (1% and 1%, respectively) compared with retrospective data analysis or cross-sectional studies (30% and 43%, respectively). Quality measures such as international registration, sample size calculation, and multicentricity of clinical sciences are generally absent from articles. Our results suggest that doctoral schools are seriously lagging behind in both teaching and scholarly activity in terms of recent clinical research methodology. Innovation and new educational platforms are essential to improve the proportion of science-oriented physicians.
Impact of Baseline Anxiety on Well-being in People with Post-COVID-19 Condition: A Secondary Analysis
Authors: Kleine Nicholas , Kwan Angela T.H. , Le Gia Han , Guo Ziji , Phan Lee , Subramaniapillai Mehala , McIntyre Roger S.
Published: March 2024

Background

Post-COVID-19 condition (PCC) is associated with a host of psychopathological conditions including prominent anxiety symptoms. However, it is not known what effect anxious symptoms have on measures of well-being in individuals living with PCC. This study aims to evaluate anxiety’s association with measures of well-being in people with PCC.

Methods

This is a post hoc analysis utilizing data from a placebo-controlled, randomized, double-blind clinical trial assessing the effect of vortioxetine on cognitive impairment in individuals with PCC (NCT05047952). Baseline data with respect to anxiety and well-being were collected using the Generalized Anxiety Disorder Scale, 7-Item (GAD-7), and the World Health Organization (WHO) Well-Being Index, 5-Item (WHO-5), respectively. A generalized linear model (GLM) analysis on baseline GAD-7 and WHO-5 scores was conducted with age, sex, employment status, education level, previous major depressive disorder (MDD) diagnosis, and confirmed COVID-19 cases as covariates.

Results

Data was analyzed in a sample of 144 participants (N = 144). After controlling for the aforementioned covariates, the results found that GAD-7 and WHO-5 scores had a significant negative correlation (β = −0.053, p = <0.001), signifying that increased anxiety had adverse effects on the overall well-being of individuals with PCC.

Conclusion

Herein, we observed a clinically meaningful level of anxiety in individuals with PCC. We also identified a robust correlation between anxiety in PCC and measures of general well-being. Our results require replication, providing the impetus for recommending screening and targeting anxious symptoms as a tactic to improve general well-being and outcomes in individuals with PCC.
The Politicisation of Constitutional Review of Memory Laws
Authors: Rhein-Fischer Paula , Wójcik Anna
Published: March 2024

German Federal Constitutional Court’s review of memory laws – Poland’s Constitutional Tribunal review of memory laws – politicisation of the judicial review of memory laws – gouvernement des juges – abusive judicial review
Common ragweed (Ambrosia artemisiifolia L.) accessions in the Mid-Atlantic region resistant to ALS-, PPO-, and EPSPS-inhibiting herbicides
Authors: D’Amico Frank , Besanҫon Thierry , Koehler Alyssa , Shergill Lovreet , Ziegler Melissa , VanGessel Mark
Published: March 2024

Common ragweed is a troublesome weed in many crops. Farmers and crop advisors in the coastal Mid-Atlantic region have reported inadequate control of common ragweed in soybean fields with glyphosate and other herbicide modes of action. To determine whether herbicide resistance was one of the causes of poor herbicide performance, 29 accessions from four states (Delaware, Maryland, New Jersey, and Virginia) where common ragweed plants survived herbicide applications and produced viable seeds were used for greenhouse screening. Common ragweed seedlings from those accessions were treated with multiple rates of cloransulam, fomesafen, or glyphosate, applied individually postemergence (POST). All accessions except one demonstrated resistance to at least one of the herbicides applied at twice the effective rate (2×), 17 accessions were two-way resistant (to glyphosate and cloransulam, or to glyphosate and fomesafen), and three-way resistance was present in eight accessions collected from three different states. Based on the POST study, five accessions were treated preemergence (PRE) with herbicides that inhibit acetolactate synthase (ALS), and two accessions were treated with herbicides that inhibit protoporphyrinogen oxidase (PPO). All accessions treated PRE with the ALS inhibitors chlorimuron or cloransulam demonstrated resistance at the 2× rates. Both accessions treated PRE with the PPO inhibitor sulfentrazone had survivors at the 2× rate. When the same accessions were treated PRE with fomesafen, one had survivors at the 2× rate, and one had survivors at the 1× rate. Results from these tests confirmed common ragweed with three-way resistance to POST herbicides is widespread in the region. In addition, this is the first confirmation that common ragweed accessions in the region are also resistant to ALS- or PPO-inhibiting herbicides when applied PRE.
A New Negative Carbon Isotope Interval Caused by Manganese Redox Cycling After the Shuram Excursion
Authors: Zhang Bin , Cao Jian , Hu Kai , Liao Zhiwei , Zhang Ruijie , Zhang Yi , Shi Chunhua , Konhauser Kurt O.
Published: March 2024

Several negative C isotope excursions (CIEs) occurred at the end of the Neoproterozoic era which have been generally attributed to the oxidation of organic carbon using sulfate as the terminal electron acceptor and the subsequent release of 13C‐depleted dissolved inorganic C (DIC). Based on new analyses from the Doushantuo Formation in South China, we observe a negative C isotope excursion right after the well‐known Shuram excursion. This excursion is equivalent to the ∼550 Ma negative CIE which is globally expressed within several continental margins. However, the origins of this CIE in the termination of Ediacaran remain unresolved. Here, we hypothesize that this post‐Shuram negative CIE was caused by a localized manganese cycling that began with the oxidation of hydrothermal Mn(II) in a water column to insoluble Mn(IV)‐oxide, followed by accumulation of Mn(IV)‐oxide to the seafloor and its subsequent dissolution via Mn(IV) reduction leading to the release of dissolved Mn(II) and 13C‐depleted DIC into ambient seawaters. This ultimately led to the precipitation of particulate Mn(II)‐carbonate characterized by low δ13Ccarb values ranging from −11.1‰ to −2.8‰. The presence of microbial fabrics in association with the Mn(II)‐carbonate further suggests that Mn(II)‐carbonate precipitation took place at the seafloor in shallow sun‐lit waters rather than in the deeper sediment pile, which archived ambient seawater C isotopic signal. Although most Ediacaran negative CIEs were generally attributed to sulfate reduction, our findings suggest that at a local level, Mn cycling can also lead to negative CIE in the Neoproterozoic, and potentially at other times in Earth's history.
Strong Climate Control on the Millennial‐Scale Dust Variability and Sediment Provenances in the Equatorial Indian Ocean Inferred From Sr‐Nd Isotopes
Authors: Shukla Arvind , Singh Sunil Kumar , Singh Dharmendra Pratap , Sharma Aka , Dimri A. P.
Published: March 2024

High‐resolution Sr and Nd isotope compositions along with major and trace element abundances have been analyzed in silicate fraction of sediments core, SSD004‐GC03, from the Equatorial Indian Ocean (7.2°N and 77.9°E) at 1,540 m water depth with a depositional history of ∼38 ka to determine source variabilities and their controlling factors. 87Sr/86Sr (0.71978–0.72491), ƐNd (−14.8 to −21.9), and a couple of source diagnostic elements display profound variability over the depositional time scale and point toward major sediment contribution from the Peninsular Gneissic Complex (PGC) and the Deccan Basalts along with aeolian dust flux, their relative proportions being determined by climate variability. The cold/arid periods are characterized by an enhanced proportion of aeolian dust and the Deccan Basalts, whereas the sediment contribution from the PGC is augmented during the warm/humid periods. The sediment provenance variations at the Equatorial Indian Ocean coincide very well with known cold/arid (Heinrich Stadial events: HS 1–4, LGM, Younger Dryas, 8.2 ka, 5.2 ka, and 1.1 ka) and warm/humid (Early Deglacial, Holocene Intensified Monsoon) climatic events reported in the tropical region and sea‐level change which are strongly captured by the Sr‐Nd isotope and elemental composition of sediments. The present investigation underscores the significant role of climate, mainly the aridity, in modulating the dust fluxes and erosion intensity and the strong coupling between Indian monsoon and North Atlantic climatic oscillations and further demonstrates minimal time delay between the production and transport of sediment from source to sink.
New insights into the clinical and molecular spectrum of the MADD-related neurodevelopmental disorder
Authors: Abdel-Salam Ghada M. H. , Abdel-Hamid Mohamed S.
Published: March 2024

Biallelic pathogenic variants in MADD lead to a very rare neurodevelopmental disorder which is phenotypically pleiotropic grossly ranging from severe neonatal hypotonia, failure to thrive, multiple organ dysfunction, and early lethality to a similar but milder phenotype with better survival. Here, we report 5 patients from 3 unrelated Egyptian families in whom 4 patients showed the severe end of the spectrum displaying neonatal respiratory distress, hypotonia and chronic diarrhea while one patient presented with the mild form displaying moderate intellectual disability and myopathy. In addition, we observed distal arthrogryposis and nonspecific structural brain anomalies in all our patients. Interestingly, cerebellar and brainstem hypoplasia were noted in one patient. Whole exome sequencing identified three novel homozygous variants in the MADD gene: two likely pathogenic [c.4321delC p.(Gln1441ArgfsTer46) and c.2620 C > T p.(Arg874Ter)] and one variant of uncertain significance (c.4307 G > A, p.Arg1436Gln). The variants segregated with the disease in all available family members. Our findings confirm that arthrogryposis, genital, cardiac and structural brain anomalies are manifestations of MADD which expand the spectrum of MADD-related neurodevelopmental disorder. Moreover, they further highlight the convergence of MADD variants on different organ systems leading to complex phenotypes.
A bird’s eye view on the use of whole exome sequencing in rare congenital ophthalmic diseases
Authors: Zucco Jessica , Baldan Federica , Allegri Lorenzo , Bregant Elisa , Passon Nadia , Franzoni Alessandra , D’Elia Angela Valentina , Faletra Flavio , Damante Giuseppe , Mio Catia
Published: March 2024

Phenotypic and genotypic heterogeneity in congenital ocular diseases, especially in anterior segment dysgenesis (ASD), have created challenges for proper diagnosis and classification of diseases. Over the last decade, genomic research has indeed boosted our understanding in the molecular basis of ASD and genes associated with both autosomal dominant and recessive patterns of inheritance have been described with a wide range of expressivity. Here we describe the molecular characterization of a cohort of 162 patients displaying isolated or syndromic congenital ocular dysgenesis. Samples were analyzed with diverse techniques, such as direct sequencing, multiplex ligation-dependent probe amplification, and whole exome sequencing (WES), over 20 years. Our data reiterate the notion that PAX6 alterations are primarily associated with ASD, mostly aniridia, since the majority of the cohort (66.7%) has a pathogenic or likely pathogenic variant in the PAX6 locus. Unexpectedly, a high fraction of positive samples (20.3%) displayed deletions involving the 11p13 locus, either partially/totally involving PAX6 coding region or abolishing its critical regulatory region, underlying its significance. Most importantly, the use of WES has allowed us to both assess variants in known ASD genes (i.e., CYP1B1, ITPR1, MAB21L1, PXDN, and PITX2) and to identify rarer phenotypes (i.e., MIDAS, oculogastrointestinal-neurodevelopmental syndrome and Jacobsen syndrome). Our data clearly suggest that WES allows expanding the analytical portfolio of ocular dysgenesis, both isolated and syndromic, and that is pivotal for the differential diagnosis of those conditions in which there may be phenotypic overlaps and in general in ASD.
Structured lifestyle modification as an adjunct to obesity pharmacotherapy: there is much to learn
Authors: Murphy Enda , Finucane Francis Martin
Published: March 2024

We are at the start of an exciting new era of very effective pharmacotherapy for patients with obesity, with the latest generation of drugs approaching the efficacy of obesity surgery. Clinical trials of obesity drugs tend to emphasise the importance of participation in some form of structured lifestyle intervention for all trial participants. This usually consists of advice to reduce calorie intake and increase moderate to vigorous physical activity. There is strong evidence that structured lifestyle modification programmes improve health in patients with obesity and related disorders. However, there is no specific evidence that they improve the response to obesity medications. This is because of the way that drug trials for patients with obesity have traditionally been designed, with participants in the active drug treatment group being compared to participants on placebo drug treatment, but with both groups always receiving the same structured lifestyle intervention. While this approach is entirely reasonable, it makes it impossible to draw any inferences about the efficacy of structured lifestyle modification to augment the response to drug therapy. Given this genuine equipoise, a critical step in ensuring that our treatment of patients with obesity is robustly evidence-based is to determine whether “drug plus lifestyle” offer any advantage over “drug plus placebo” in large, well-designed and adequately powered clinical trials. We also need to determine the cost-effectiveness of these programmes.
Hematopoietic cell transplantation and cell therapy activity landscape survey in the Kingdom of Saudi Arabia; a report from the Saudi Society of Blood and Marrow Transplantation (SSBMT)
Authors:   On behalf of Saudi Society of Blood & Marrow Transplantation (SSBMT), Shaheen Naila , Abosoudah Ibrahim , Alshahrani Mohammad , Alzahrani Mohsen , Essa Mohammed , Alahmari Bader , Mutaher Enas , Kanfar Solaf , Alsaeed Ahmad , Alamoudi Sameer , Monagel Dania , Marei Mohammed , Alzahrani Musa , Alsultan Abdulrahman , Aljefri Abdullah , Masari Ahlam , Alsharif Omer , Alsughayir Ammar H. , Hejazi Ayman , Aldaama Saad , Alaskar Ahmed
Published: March 2024

Hematopoietic Cell Transplantation (HCT) activity was surveyed in the Kingdom of Saudi Arabia (KSA). The overall rate of HCT per 10,000,000 inhabitants doubled every 10 years. 15,031 HCTs were reported by all the functional HCT centers in KSA since inception of HCT program. Out of total HCT 15,031; 10,232(68%) were reported in adults, and 4799(32%) in the pediatric population. Allogeneic HCT constituted 10,489(70%) of total HCT, with majority from Human Leukocyte Antigen matched identical sibling (85.4%). The autologous HCTs were 4542(30%). During the last five years 2018–2022; in total 5164 HCTs were performed, with the majority had allogeneic HCT 3,085(59.74%), followed by the autologous HCT 3085(40.2%). The top three main indications of the autologous HCT were Multiple Myeloma 299(28%), Hodgkin Lymphoma 293(27.8%), and Non-Hodgkin Lymphoma 212(20%). Hemoglobinopathies 615(27.6%) were mostly indicated for allogeneic HCT, followed by Acute Myeloid Leukemia 433(19.4%), and Precursors Lymphoid Neoplasms 322(14.4%). The HCT activity landscape survey provides the updated current state and trends for HCT in KSA. The reported HCT numbers differ than what was reported by international registries, since not all the cases have been reported. We urge to have a common data hub nationally in order to capture the actual number of cases.
Investigating the cut-off values of captopril challenge test for primary aldosteronism using the novel chemiluminescent enzyme immunoassay method: a retrospective cohort study
Authors: Tezuka Yuta , Omata Kei , Ono Yoshikiyo , Kambara Kengo , Kamada Hiroki , Oguro Sota , Yamazaki Yuto , Gomez-Sanchez Celso E. , Ito Akihiro , Sasano Hironobu , Takase Kei , Tanaka Tetsuhiro , Katagiri Hideki , Satoh Fumitoshi
Published: March 2024

The measurement evolution enabled more accurate evaluation of aldosterone production in hypertensive patients. However, the cut-off values for novel assays have been not sufficiently validated. The present study was undertaken to validate the novel chemiluminescent enzyme immunoassay for aldosterone in conjunction with other methods. Moreover, we also aimed to establish a new cut-off value for primary aldosteronism in the captopril challenge test using the novel assay. First, we collected 390 plasma samples, in which aldosterone levels measured using liquid chromatography-mass spectrometry ranged between 0.18 and 1346 ng/dL. The novel chemiluminescent enzyme immunoassay showed identical correlation of plasma aldosterone with liquid chromatography-mass spectrometry, in contrast to conventional radioimmunoassay. Further, we enrolled 299 and 39 patients with primary aldosteronism and essential hypertension, respectively. Plasma aldosterone concentrations measured using the novel assay were lower than those measured by radioimmunoassay, which resulted in decreased aldosterone-to-renin ratios. Subsequently, positive results of the captopril challenge test based on radioimmunoassay turned into “negative” based on the novel assay in 45% patients with primary aldosteronism, using the conventional cut-off value (aldosterone-to-renin activity ratio > 20 ng/dL per ng/mL/h). Receiver operating characteristic curve analysis demonstrated that aldosterone-to-renin activity ratios > 8.2 ng/dL per ng/mL/h in the novel assay was compatible with the conventional diagnosis (sensitivity, 0.874; specificity, 0.980). Our study indicates the great measurement accuracy of the novel chemiluminescent enzyme immunoassay for aldosterone, and the importance of measurement-adjusted cut-offs in the diagnosis of primary aldosteronism.
Modulation of DBS-induced cortical responses and movement by the directionality and magnitude of current administered
Authors: Spooner Rachel K. , Hizli Baccara J. , Bahners Bahne H. , Schnitzler Alfons , Florin Esther
Published: March 2024

Subthalamic deep brain stimulation (STN-DBS) is an effective therapy for alleviating motor symptoms in people with Parkinson’s disease (PwP), although some may not receive optimal clinical benefits. One potential mechanism of STN-DBS involves antidromic activation of the hyperdirect pathway (HDP), thus suppressing cortical beta synchrony to improve motor function, albeit the precise mechanisms underlying optimal DBS parameters are not well understood. To address this, 18 PwP with STN-DBS completed a 2 Hz monopolar stimulation of the left STN during MEG. MEG data were imaged in the time-frequency domain using minimum norm estimation. Peak vertex time series data were extracted to interrogate the directional specificity and magnitude of DBS current on evoked and induced cortical responses and accelerometer metrics of finger tapping using linear mixed-effects models and mediation analyses. We observed increases in evoked responses (HDP ~ 3–10 ms) and synchronization of beta oscillatory power (14–30 Hz, 10–100 ms) following DBS pulse onset in the primary sensorimotor cortex (SM1), supplementary motor area (SMA) and middle frontal gyrus (MFG) ipsilateral to the site of stimulation. DBS parameters significantly modulated neural and behavioral outcomes, with clinically effective contacts eliciting significant increases in medium-latency evoked responses, reductions in induced SM1 beta power, and better movement profiles compared to suboptimal contacts, often regardless of the magnitude of current applied. Finally, HDP-related improvements in motor function were mediated by the degree of SM1 beta suppression in a setting-dependent manner. Together, these data suggest that DBS-evoked brain-behavior dynamics are influenced by the level of beta power in key hubs of the basal ganglia-cortical loop, and this effect is exacerbated by the clinical efficacy of DBS parameters. Such data provides novel mechanistic and clinical insight, which may prove useful for characterizing DBS programming strategies to optimize motor symptom improvement in the future.
Implementation of pooled saliva tests for universal screening of cCMV infection
Authors: Merav Lior , Ofek Shlomai Noa , Oiknine-Djian Esther , Caplan Orit , Livneh Ayala , Sido Tal , Peri Amir , Shtoyer Aviad , Amir Eden , Ben Meir Kerem , Daitch Yutti , Rivkin Mila , Kripper Esther , Fogel Irit , Horowitz Hadar , Greenberger Sraya , Cohen Mevaseret , Geal-Dor Miriam , Gordon Oren , Averbuch Diana , Ergaz-Shaltiel Zivanit , Eventov Friedman Smadar , Wolf Dana G. , Yassour Moran
Published: March 2024

Congenital cytomegalovirus (cCMV) is the most common intrauterine infection, leading to neurodevelopmental disabilities. Universal newborn infant screening of cCMV has been increasingly advocated. In the absence of a high-throughput screening test, which can identify all infected newborn infants, the development of an accurate and efficient testing strategy has remained an ongoing challenge. Here we assessed the implementation of pooled saliva polymerase chain reaction (PCR) tests for universal screening of cCMV, in two hospitals of Jerusalem from April 2022 through April 2023. During the 13-month study period, 15,805 infants (93.6% of all live newborn infants) were screened for cCMV using the pooled approach that has since become our routine screening method. The empirical efficiency of the pooling was six (number of tested newborn infants per test), thereby sparing 83% of the saliva tests. Only a minor 3.05 PCR cycle loss of sensitivity was observed for the pooled testing, in accordance with the theoretical prediction for an eight-sample pool. cCMV was identified in 54 newborn infants, with a birth prevalence of 3.4 per 1,000; 55.6% of infants identified with cCMV were asymptomatic at birth and would not have been otherwise targeted for screening. The study demonstrates the wide feasibility and benefits of pooled saliva testing as an efficient, cost-sparing and sensitive approach for universal screening of cCMV.
Structure and interactions of the endogenous human Commander complex
Authors: Laulumaa Saara , Kumpula Esa-Pekka , Huiskonen Juha T. , Varjosalo Markku
Published: March 2024

The Commander complex, a 16-protein assembly, plays multiple roles in cell homeostasis, cell cycle and immune response. It consists of copper-metabolism Murr1 domain proteins (COMMD1–10), coiled-coil domain-containing proteins (CCDC22 and CCDC93), DENND10 and the Retriever subcomplex (VPS26C, VPS29 and VPS35L), all expressed ubiquitously in the body and linked to various diseases. Here, we report the structure and key interactions of the endogenous human Commander complex by cryogenic-electron microscopy and mass spectrometry-based proteomics. The complex consists of a stable core of COMMD1–10 and an effector containing DENND10 and Retriever, scaffolded together by CCDC22 and CCDC93. We establish the composition of Commander and reveal major interaction interfaces. These findings clarify its roles in intracellular transport, and uncover a strong association with cilium assembly, and centrosome and centriole functions.
Structure of the human outer kinetochore KMN network complex
Authors: Yatskevich Stanislau , Yang Jing , Bellini Dom , Zhang Ziguo , Barford David
Published: March 2024

Faithful chromosome segregation requires robust, load-bearing attachments of chromosomes to the mitotic spindle, a function accomplished by large macromolecular complexes termed kinetochores. In most eukaryotes, the constitutive centromere-associated network (CCAN) complex of the inner kinetochore recruits to centromeres the ten-subunit outer kinetochore KMN network that comprises the KNL1C, MIS12C and NDC80C complexes. The KMN network directly attaches CCAN to microtubules through MIS12C and NDC80C. Here, we determined a high-resolution cryo-EM structure of the human KMN network. This showed an intricate and extensive assembly of KMN subunits, with the central MIS12C forming rigid interfaces with NDC80C and KNL1C, augmented by multiple peptidic inter-subunit connections. We also observed that unphosphorylated MIS12C exists in an auto-inhibited state that suppresses its capacity to interact with CCAN. Ser100 and Ser109 of the N-terminal segment of the MIS12C subunit Dsn1, two key targets of Aurora B kinase, directly stabilize this auto-inhibition. Our study indicates how selectively relieving this auto-inhibition through Ser100 and Ser109 phosphorylation might restrict outer kinetochore assembly to functional centromeres during cell division.
A chromosome-level genome assembly of yellow stem borer (Scirpophaga incertulas)
Authors: Zhou Sicong , Luo Guanghua , Yang Qiong , Han Yangchun , Yuan Kaili , Ji Rui , Fang Jichao
Published: March 2024

The yellow stem borer Scirpophaga incertulas is the dominant pest of rice in tropical Asia. However, the lack of genomic resources makes it difficult to understand their invasiveness and ecological adaptation. A high-quality chromosome-level genome of S. incertulas, a monophagous rice pest, was assembled by combining Illumina short reads, PacBio HiFi long sequencing, and Hi-C scaffolding technology. The final genome size was 695.65 Mb, with a scaffold N50 of 28.02 Mb, and 93.50% of the assembled sequences were anchored to 22 chromosomes. BUSCO analysis demonstrated that this genome assembly had a high level of completeness, with 97.65% gene coverage. A total of 14,850 protein-coding genes and 366.98 Mb of transposable elements were identified. In addition, comparative genomic analyses indicated that chemosensory processes and detoxification capacity may play critical roles in the specialized host preference of S. incertulas. In summary, the chromosome-level genome assembly of S. incertulas provides a valuable genetic resource for understanding the biological characteristics of its invasiveness and developing an efficient management strategy.
Evaluating the lifetime performance index of omega distribution based on progressive type-II censored samples
Authors: Kilany N. M. , El-Refai Lobna H.
Published: March 2024

Besides achieving high quality products, statistical techniques are applied in many fields associated with health such as medicine, biology and etc. Adhering to the quality performance of an item to the desired level is a very important issue in various fields. Process capability indices play a vital role in evaluating the performance of an item. In this paper, the larger-the-better process capability index for the three-parameter Omega model based on progressive type-II censoring sample is calculated. On the basis of progressive type-II censoring the statistical inference about process capability index is carried out through the maximum likelihood. Also, the confidence interval is proposed and the hypothesis test for estimating the lifetime performance of products. Gibbs within Metropolis–Hasting samplers procedure is used for performing Markov Chain Monte Carlo (MCMC) technique to achieve Bayes estimation for unknown parameters. Simulation study is calculated to show that Omega distribution's performance is more effective. At the end of this paper, there are two real-life applications, one of them is about high-performance liquid chromatography (HPLC) data of blood samples from organ transplant recipients. The other application is about real-life data of ball bearing data. These applications are used to illustrate the importance of Omega distribution in lifetime data analysis.