bluuebunny/crossref_metadata_2025 huggingface dataset:
Spatial Transcriptomics as a Novel Approach to Redefine Electrical Stimulation Safety
Authors: Whitsitt Quentin A. , Koo Beomseo , Celik Mahmut Emin , Evans Blake M. , Weiland James D. , Purcell Erin K.
Published: July 2022

Current standards for safe delivery of electrical stimulation to the central nervous system are based on foundational studies which examined post-mortem tissue for histological signs of damage. This set of observations and the subsequently proposed limits to safe stimulation, termed the “Shannon limits,” allow for a simple calculation (using charge per phase and charge density) to determine the intensity of electrical stimulation that can be delivered safely to brain tissue. In the three decades since the Shannon limits were reported, advances in molecular biology have allowed for more nuanced and detailed approaches to be used to expand current understanding of the physiological effects of stimulation. Here, we demonstrate the use of spatial transcriptomics (ST) in an exploratory investigation to assess the biological response to electrical stimulation in the brain. Electrical stimulation was delivered to the rat visual cortex with either acute or chronic electrode implantation procedures. To explore the influence of device type and stimulation parameters, we used carbon fiber ultramicroelectrode arrays (7 μm diameter) and microwire electrode arrays (50 μm diameter) delivering charge and charge density levels selected above and below reported tissue damage thresholds (range: 2–20 nC, 0.1–1 mC/cm2). Spatial transcriptomics was performed using Visium Spatial Gene Expression Slides (10x Genomics, Pleasanton, CA, United States), which enabled simultaneous immunohistochemistry and ST to directly compare traditional histological metrics to transcriptional profiles within each tissue sample. Our data give a first look at unique spatial patterns of gene expression that are related to cellular processes including inflammation, cell cycle progression, and neuronal plasticity. At the acute timepoint, an increase in inflammatory and plasticity related genes was observed surrounding a stimulating electrode compared to a craniotomy control. At the chronic timepoint, an increase in inflammatory and cell cycle progression related genes was observed both in the stimulating vs. non-stimulating microwire electrode comparison and in the stimulating microwire vs. carbon fiber comparison. Using the spatial aspect of this method as well as the within-sample link to traditional metrics of tissue damage, we demonstrate how these data may be analyzed and used to generate new hypotheses and inform safety standards for stimulation in cortex.
A Nomogram Based on Hematological Parameters and Clinicopathological Characteristics for Predicting Local–Regional Recurrence After Breast-Conserving Therapy
Authors: Sun Luhao , Zhao Wei , Wang Fukai , Song Xiang , Wang Xinzhao , Li Chao , Yu Zhiyong
Published: July 2022

Objectives

The aim of this study was to identify the factors for local–regional recurrence (LRR) after breast-conserving therapy (BCT). We established a practical nomogram to predict the likelihood of LRR after BCT based on hematological parameters and clinicopathological features.

Methods

A retrospective analysis was performed on 2,085 consecutive breast cancer patients who received BCT in Shandong Cancer Hospital from 2006 to 2016, including 1,460 patients in the training cohort and 625 patients in the validation cohort. Univariate and multivariate analyses were performed based on hematological parameters (fibrinogen, platelets, mean platelet volume, neutrophils, monocytes, and lymphocytes) and clinicopathological characteristics to identify the independent factors for LRR. Subsequently, a nomogram for predicting LRR was established by logistic regression analysis. The nomogram was validated in 625 patients in the validation cohort.

Results

During the median follow-up period of 66 months, 44 (3.01%) patients in the training cohort and 19 (3.04%) patients in the validation cohort suffered from LRR. Multivariate analysis showed six independent factors related to LRR, including molecular subtype, pathological N stage, re-resection, radiotherapy or not, platelet count*MPV*fibrinogen (PMF), and neutrophil count/lymphocyte count ratio (NLR). Six variables were entered into logistic regression to establish the nomogram for predicting LRR. The nomogram of LRR showed excellent discrimination and prediction accuracy. The area under the receiver operating characteristic curve (AUC) was 0.89 (p < 0.001, 95% CI = 0.83, 0.95) in the training cohort and 0.88 (p < 0.001, 95% CI = 0.8, 0.96) in the validation cohort. Calibration curves for the prediction model in the training and validation cohorts both demonstrated satisfactory consistency between the nomogram-predicted and actual LRR.

Conclusion

The combination of hematological parameters and clinicopathological characteristics can predict LRR after BCT. The predictive nomogram based on preoperative and postoperative indicators of BCT might serve as a practical tool for individualized prognostication. More prospective studies should be performed to verify the model.
Image-guided interstitial brachytherapy for recurrent cervical cancer after radiotherapy: A single institution experience
Authors: Ren Xiaojun , Fu Yingli , Liu Zhongshan , Lin Xia , Qiu Ling , Li Yunfeng , Li Hanyang , Bai Yuqi , Wang Tiejun
Published: July 2022

Purpose

The aim of this study is to evaluate the efficacy and toxicity of image-guided high-dose rate (HDR) interstitial brachytherapy (ISBT) for the reirradiation of cervical cancer within a previously irradiated area.

Methods and materials

Twenty-three consecutive patients with cervical cancer were reirradiated with curative intent using brachytherapy (BT) with or without external beam irradiation. The median biologically equivalent dose in 2-Gy fractions (EQD2) for reirradiation was 64.0 Gy (range: 31.3–95.1 Gy), and the median cumulative EQD2 (for primary treatment and reirradiation) was 152.4 Gy (range: 97.8–200.9 Gy). The average clinical target volume was 82.9 cm3 (range: 26.9–208.3 cm3), and the median treatment-free interval (TFI) was 13 months (range: 3–93 months).

Results

The median follow-up time was 19 months (range: 2–59 months). The complete response rate after reirradiation was 56.5%. The 1-, 2- 3-, and 4-year post-relapse survival (PRS) rates were 65.2%, 43.5%, 33.8%, and 27.1%, respectively. The median reirradiation EQD2 D2cc of rectum and bladder was 39.5 Gy (range = 14.6–96.2 Gy) and 52.1 Gy (range = 29.1–114.2 Gy). The median cumulative EQD2 D2cc of rectum and bladder was 115.0 Gy (range = 84.4–189.3 Gy) and 130.5 Gy (range = 95.5–173.5 Gy). During follow-up, nine (39.1%) patients had experienced grade 3 or 4 late toxicities. Grade ≥3 rectal toxicity occurred in three patients (13.0%). Grade ≥3 urinary toxicity occurred in five patients (21.7%). One patient (4.3%) had both grade ≥3 urinary and rectal toxicity. Tumor volume, TFI, tumor invasion organ number, and local control were significant prognostic factors adversely affecting OS.

Conclusions

For recurrent cervical cancer after radiotherapy, reirradiation of HDR-ISBT is feasible, even if the local tumor invasion is large, with a good chance of survival and acceptable side effects.
Rechallenge of denosumab in advanced giant cell tumor of the bone after atypical femur fracture: A case report and review of literature
Authors: Nasca Vincenzo , Frezza Anna Maria , Morosi Carlo , Buonomenna Ciriaco , Parafioriti Antonina , Zappalà Giorgio , Bini Federica , Casali Paolo Giovanni , Loppini Mattia , Stacchiotti Silvia
Published: July 2022

Giant cell tumor of the bone (GCTB) is a locally aggressive neoplasm where surgery is often curative. However, it can rarely give rise to distant metastases. Currently, the only available active therapeutic option for unresectable GCTB is denosumab, an anti-RANKL monoclonal antibody that dampens the aggressive osteolysis typically seen in this disease. For advanced/metastatic GCTB, denosumab should be continued lifelong, and although it is usually well tolerated, important questions may arise about the long-term safety of this drug. In fact, uncommon but severe toxicities can occur and eventually lead to denosumab discontinuation, such as atypical fracture of the femur (AFF). The optimal management of treatment-related AFF is a matter of debate, and to date, it is unknown whether reintroduction of denosumab at disease progression is a clinically feasible option, as no reports have been provided so far. Hereinafter, we present a case of a patient with metastatic GCTB who suffered from AFF after several years of denosumab; we describe the clinical features, orthopedic treatment, and oncological outcomes, finally providing the first evidence that denosumab rechallenge after AFF occurrence may be a safe and viable option at GCTB progression.
Nivolumab Versus Sorafenib as First-Line Therapy for Advanced Hepatocellular Carcinoma: A Cost-Effectiveness Analysis
Authors: Li Yan , Liang Xueyan , Li Huijuan , Yang Tong , Guo Sitong , Chen Xiaoyu
Published: July 2022

Objective: Nivolumab improves overall survival (OS) and is associated with fewer adverse events than sorafenib for the treatment of advanced hepatocellular carcinoma (aHCC). However, the cost-effectiveness of nivolumab compared with sorafenib treatment for aHCC remains unclear. This study evaluated the cost-effectiveness of nivolumab and sorafenib in the treatment of aHCC.

Materials and methods: A partitioned survival model that included three mutually exclusive health states was used to evaluate the cost-effectiveness of nivolumab and sorafenib for treating aHCC. The clinical characteristics and outcomes of the patients in the model were obtained from the CheckMate 459. We performed deterministic one-way sensitivity and probabilistic sensitivity analyses to evaluate the robustness of the model. Subgroup analyses were also performed. Costs, life-years, quality-adjusted life-years (QALYs), incremental cost-effectiveness ratio (ICER), incremental net health benefits (INHB), and incremental net monetary benefits (INMB) were measured.

Results: The base case analysis showed that compared with sorafenib, treatment with nivolumab was associated with an increment of 0.50 (2.45 vs. 1.95) life-years and an increment of 0.32 (1.59 vs. 1.27) QALYs, as well as a $69,762 increase in cost per patient. The ICER was $220,864/QALY. The INHB and INMB were −0.15 QALYs and −$22,362 at a willingness-to-pay (WTP) threshold of $150,000/QALY, respectively. The probabilistic sensitivity analysis demonstrated that the probability of nivolumab being cost-effective was only 10.38% at a WTP threshold of $150,000/QALY. The model was most sensitive to the costs of sorafenib and nivolumab according to the one-way sensitivity analysis. When the price of sorafenib exceeded $0.93/mg or nivolumab was less than $24.23/mg, nivolumab was more cost-effective. The subgroup analysis illustrated that the probability of cost-effectiveness was >50% in the Barcelona Clinic Liver Cancer Stage B subgroups for nivolumab at a WTP threshold of $150,000/QALY. This study also showed that the probability of cost-effectiveness was <50% in most subgroups.

Conclusion: Nivolumab was not cost-effective, although it was associated with better clinical benefit and a favorable safety profile for the treatment of aHCC compared with sorafenib from the third-party payer perspective in the United States. If the price of nivolumab is substantially reduced, favorable cost-effectiveness can be achieved among patients with aHCC.
A UPLC-Q-TOF-MS-Based Metabolomics Approach to Screen out Active Components in Prepared Rhubarb for Its Activity on Noxious Heat Blood Stasis Syndrome
Authors: Zhu Hui , Duan Yu , Qin Kunming , Jin Junjie , Liu Xiao , Cai Baochang
Published: July 2022

Background: Prepared rhubarb was obtained by steaming raw rhubarb with wine. Different from raw rhubarb with a purgative effect, prepared rhubarb shows effects of promoting blood circulation and removing blood stasis. However, the mechanisms of its action through regulating endogenous metabolites remain unclear.

Purpose: The purpose of this study was to explore active chemical components in prepared rhubarb for its activity on noxious heat blood stasis syndrome (NHBS) by comprehensive metabolomics profiling.

Study design: Plant extracts usually show their activities in a synergistic way; therefore, integrated omics was developed as a rational way for a better understanding of their biological effects and potential active compounds.

Methods: The activities of prepared rhubarb were evaluated by biochemical and metabolomic analysis; meanwhile, serum chemical profiles were sought using UHPLC-Q-TOF-MS. Gray correlation analysis (GCA) was used for calculating the underlying correlations between them.

Results: The metabolomics profiles of rat plasma from model and control groups were significantly different, with 31 endogenous metabolites changed by NHBS. Then, after the administration of prepared rhubarb, 18 of them were regulated. Multiple metabolic pathways were disturbed after NHBS modeling and restored by prepared rhubarb, among which had a greater impact on sphingolipid metabolism. A total of 28 compounds from prepared rhubarb absorbed into the plasma were identified, including nine prototypes and 19 metabolites. Statistical results suggested that rhein and its metabolites accounted for half of the top 10 active compounds in prepared rhubarb for its biomedical activities.

Conclusion: This study presented evidence for the therapeutic effects and active chemicals of prepared rhubarb on NHBS in the way of metabolomics.
The Relationship of Pyroptosis-Related Genes, Patient Outcomes, and Tumor-Infiltrating Cells in Bladder Urothelial Carcinoma (BLCA)
Authors: Xie Ruiyan , Xie Ming , Zhu Litong , Chiu Joanne W. Y. , Lam Wayne , Yap Desmond Y. H.
Published: July 2022

Introduction: The role of pyroptosis and its effects on tumor-infiltrating cells (TICs) in the pathogenesis and treatment outcomes of patients with bladder urothelial carcinoma (BLCA) remains unclear.

Methods: We conducted a bioinformatics analysis on the pyroptosis-related genes (PRGs) and TICs using data from public domains, and evaluated their impact on the pathogenesis and clinical outcomes of BLCA patients. A risk score based on PRGs and a prognostic risk model that incorporated patient demographics, tumor characteristics, and differentially expressed genes (DEGs) were developed.

Results: Twenty-three DEGs of 52 PRGs were identified in BLCA and normal samples from the TCGA database. Missense mutations and single nucleotide polymorphisms in PRGs are the most common genetic abnormalities. Patients with high PRG risk scores showed an inferior survival compared to those with low risk scores. The prognostic risk model based on patient demographics, tumor characteristics, and DEGs showed good predictive values for patient survival at 1, 3, and 5 years in BLCA patients. Caspase-8 (CASP8) was the only intersection gene of the prognostic genes, DEGs, and different genes expressed in tissue. Patients with a high CASP8 expression had improved survival, and an increased CASP8 expression level was observed in activated CD4 memory T cells, follicular T helper cells, resting NK cells, M0 macrophages, and activated dendritic cells. CASP8 expression also showed a positive correlation with the IL7R expression—a key cell marker of CD4 memory T cells. CASP8 expression also showed correlations with immune checkpoints (PDCD1, CD274, and CTLA4) and response to immune checkpoint inhibitors.

Conclusion: Our data suggest that PRGs, especially CASP8, showed strong associations with patient outcomes and TICs in BLCA. If validated, these results could potentially aid in the prognostication and guide treatment in BLCA patients.
Probing Genuine Multipartite Einstein–Podolsky–Rosen Steering and Entanglement Under an Open Tripartite System
Authors: Sun Wen-Yang , Ding Amin , Gao Haitao , Wang Le , He Juan , Ye Liu
Published: July 2022

Einstein–Podolsky–Rosen steering is a peculiar quantum nonlocal correlation and has unique physical characteristics and a wide application prospect. Even more importantly, multipartite steerable states have more vital applications in the future quantum information field. Thus, in this work, we explored the dynamics characteristics of both genuine multipartite steering (GMS) and genuine multipartite entanglement (GME) and the relations of both under an open tripartite system. Specifically, the tripartite decoherence system may be modeled by the three parties of a tripartite state that undergo the noisy channels. The conditions for genuine entangled and steerable states can be acquired for the initial tripartite state. The results showed that decoherence noises can degrade the genuine multipartite entanglement and genuine multipartite steering and even induce its death. Explicitly, GME and GMS disappear with the increase in the decoherence strength under the phase damping channel. However, GME and GMS rapidly decay to death with the increase in the channel-noise factor and then come back to life soon after in the bit flip channel. Additionally, the results indicate that GMS is born of GME, but GME does not imply GMS, which means that the set of genuine multipartite steerable states is a strict subset of the set of genuine multipartite entangled states. These conclusions may be useful for discussing the relationship of quantum nonlocal correlations (GME and GMS) in the decoherence systems.
Fertilization reduces root architecture plasticity in Ulmus pumila used for afforesting Mongolian semi-arid steppe
Authors: Montagnoli Antonio , Lasserre Bruno , Terzaghi Mattia , Byambadorj Ser-Oddamba , Nyam-Osor Batkhuu , Scippa Gabriella Stefania , Chiatante Donato
Published: July 2022

In this study, we assessed the functional and architectural traits in the coarse roots of Ulmus pumila trees, which are used for afforesting the semi-arid steppe of Mongolia. Tree growth was supported by different watering regimes (no watering, 2, 4, and 8 L h−1) and by two types of soil fertilization (NPK and compost). In July, 2019, for each of these treatments six trees, outplanted in 2011 as 2-year-old seedlings from a container nursery, were randomly selected, excavated by hand, and digitized. The build-up of root length correlated positively with increasing levels of watering for both soil depths analyzed. The application of fertilizers led to root growth suppression resulting in a general reduction of root length in a lowered rooting depth. When root system characteristics were analyzed in relation to wind direction, unfertilized trees showed higher root diameter values in both soil layers of leeward quadrants, likely a response to mechanical forces to improve stability. On the contrary, fertilized trees did not show differences in root diameter among the different quadrants underscoring a strong reduction in root plasticity with a lack of morpho-architectural response to the mechanical forces generated by the two prevailing winds. Finally, the root branching density, another important trait for fast dissipation of mechanical forces, was significantly reduced by the fertilization, independently of the quadrants and watering regime. Our results suggest that knowledge of the root response to the afforestation techniques applied in the semi-arid steppe of Mongolia is a necessary step for revealing the susceptibility of this forest shelterbelt to the exacerbating environmental conditions caused by climate change and, thus, to the development of a sustainable and successful strategy to restore degraded lands.
Overexpression of ZmIPT2 gene delays leaf senescence and improves grain yield in maize
Authors: Song Yongfeng , Li Chunxiang , Zhu Yong , Guo Pei , Wang Qi , Zhang Lin , Wang Zhenhua , Di Hong
Published: July 2022

Cytokinins (CTKs) are a major phytohormone group that are significant in the promotion of cellular division, growth, and divergence. Isopentenyl transferase (IPT) regulates a rate-limiting step in plant CTK synthesis, promotes the synthesis of isopentenyl adenonucleotides from 5-AMP and isopentenyl pyrophosphate, and then converts both these chemicals into various CTKs. Here, the full-length cDNA of ZmIPT2, which encodes 322 amino acids, was isolated and was introduced into a maize inbred line by Agrobacterium-mediated transformation. In both controlled environments and field experiments, the overexpression of ZmIPT2 gene in the transformed plants delayed leaf senescence. Compared to the receptor line, the transgenic maize lines retained higher chlorophyll levels, photosynthetic rates, and cytokinin content for an extended period of time, and produced significantly higher grain yield by a margin of 17.71–20.29% under normal field planting conditions. Subsequently, ten possible genes that interacted with ZmIPT2 were analyzed by qRT-PCR, showing that the expression pattern of GRMZM2G022904 was consistent with ZmIPT2 expression. Through comprehensive analysis, we screened for transgenic lines with stable inheritance of ZmIPT2 gene, clear functional efficiency, and significant yield improvement, in order to provide theoretical basis and material support for the breeding of new high-yield transgenic maize varieties.
Entrepreneurial Leadership and Employee Wellbeing During COVID-19 Crisis: A Dual Mechanism Perspective
Authors: Bilal Muhammad , Chaudhry Shafaq Arif , Sharif Imran , Shafique Owais , Shahzad Khurram
Published: July 2022

This study examined the potential impacts of entrepreneurial leadership on followers' psychological wellbeing and proactive work behavior through sustainable employability and work uncertainty in a sample of 218 employees employed in SMEs of Pakistan. Hierarchical regression results demonstrated that entrepreneurial leadership was positively connected with sustainable employability and negatively linked with work uncertainty. Sequentially, sustainable employability was positively correlated with proactive work behavior and employees' psychological wellbeing, and work uncertainty was negatively associated with proactive work behavior and employees' psychological wellbeing during the COVID-19 crisis. Furthermore, bootstrapping confirmed the mediation effects of work uncertainty and sustainable employability on proactive work behavior and the psychological wellbeing of employees. Sustainable employability did not mediate the relationship between entrepreneurial leadership and psychological wellbeing. Mediators, sustainable employability, and work uncertainty positively linked employees' psychological wellbeing and proactive work behavior. The results highlighted the significant roles of sustainable employability and work uncertainty and interpreted why entrepreneurial leadership may affect employees' positive behaviors.
I Like the Food You Made! Overly Positive Feedback Is Most Likely Given to Those That Want to Excel in a Task and Handle Failure Badly
Authors: Cantarero Katarzyna , Byrka Katarzyna , Kosiarczyk Aleksandra , Dolinski Dariusz
Published: July 2022

In this article, we focus on how people resolve the dilemma between honest feedback and a prosocial lie depending on the context. In a pre-registered study (N = 455), we asked participants to choose between telling the blatant truth or lying prosocially regarding a dish made poorly by a stranger. The results showed that participants were most eager to pass on overly positive feedback when the stranger cared about cooking and was very sensitive to negative feedback. Perceived harm in truth telling mediated the relationship between desire to excel in a task with high ability to handle failure and choosing a prosocial lie.
The Gap Between the Perceptions and Expectations of Quality of Services Delivered to the Participants at the Scientific Conferences Using the University of Hafr Al Batin as a Model
Authors: Alharbi Abdullah Muzil , Alshammari Adel Ayed , Naqvi Hasnain Abbas
Published: July 2022

This study aims to reveal the gap between the perceptions and expectations of the quality of services delivered to participants at scientific conferences held at emerging Saudi universities (using University of Hafr Al Batin as a model). The study also reveals if there are differences, with statistical significance, in the sample response due to several variants such as gender, nationality, and the attendance rate of conferences per year. The research adopts the descriptive approach and uses SERVQUAL instruments. It has been applied to a random sample of 155 persons. The study outcomes show that the expectations are higher than the perceptions in terms of all dimensions and also demonstrate the differences that exist due to the attendance rate of conferences per year in favor of those who attended three or more conferences. The study provides suggestions to raise the quality of services delivered to the participants at these conferences.
Horizontal Tourism Coopetition Strategy for Marketing Performance – Evidence From Theme Parks
Authors: Wu Meiju , He Jianmin
Published: July 2022

Unprecedentedly impacted by COVID-19, tourism enterprises are pushed to adopt new strategic management to cope with the changes in tourists' consumer perception for sustainable development, such as corporate and compete simultaneously with their competitors. Our study aims to investigate the intermediate role of coopetition, including the three dimensions of resource similarity, market commonality, and willingness to cooperate in the marketing and performance relationships. Primary data on 360 observers were collectedviaquestionnaire distribution to theme park managers in China with 85.3% accuracy in response rate. Structural equation modeling (SEM) was used to verify the intermediate effect of coopetition on marketing performance in tourism enterprises. The results of SEM indicate (1) the intermediate role of tourism coopetition, including the three dimensions existing in the relationship between tourism marketing and performance, (2) more significant positive impact on non-financial performance than that on financial performance, and (3) the mechanism of implementing coopetition. This study gives supportive evidence for tourism enterprises to implement coopetition and highlights the implications for appropriately developing coopetition strategies and tactics to achieve the synergy effect for the individual enterprises and the spillover effect for the destination regarding policy-making, mindset, and partner selection.
Dexamethasone Alleviates Myocardial Injury in a Rat Model of Acute Myocardial Infarction Supported by Venoarterial Extracorporeal Membrane Oxygenation
Authors: Cheng Xingdong , Zhang Rongzhi , Wei Shilin , Huang Jian , Zhai Kerong , Li Yongnan , Gao Bingren
Published: July 2022

Myocardial ischemia causes myocardial inflammation. Research indicates that the venoarterial extracorporeal membrane oxygenation (VA ECMO) provides cardiac support; however, the inflammatory response caused by myocardial ischemia remains unresolved. Dexamethasone (Dex), a broad anti-inflammatory agent, exhibits a cardioprotective effect. This study aims to investigate the effect of Dex on a rat model of acute myocardial infarction (AMI) supported by VA ECMO. Male Sprague-Dawley rats (300–350 g) were randomly divided into three groups: Sham group (n = 5), ECMO group (n = 6), and ECMO + Dex group (n = 6). AMI was induced by ligating the left anterior descending (LAD) coronary artery. Sham group only thoracotomy was performed but LAD was not ligated. The ECMO and ECMO + Dex groups were subjected to 1 h of AMI and 2 h of VA ECMO. In the ECMO + Dex group, Dex (0.2 mg/kg) was intravenously injected into the rats after 1 h of AMI. Lastly, myocardial tissue and blood samples were harvested for further evaluation. The ECMO + Dex group significantly reduced infarct size and levels of cTnI, cTnT, and CK-MB. Apoptotic cells and the expression levels of Bax, Caspase3, and Cle-Caspase3 proteins were markedly lower in the ECMO + Dex group than that in the ECMO group. Neutrophil and macrophage infiltration was lower in the ECMO + Dex group than in the ECMO group. A significant reduction was noted in ICAM-1, C5a, MMP-9, IL-1β, IL-6, and TNF-α. In summary, our findings revealed that Dex alleviates myocardial injury in a rat model of AMI supported by VA ECMO.
Radiative Transfer Speed-Up Combining Optimal Spectral Sampling With a Machine Learning Approach
Authors: Mauceri Steffen , O’Dell Christopher W. , McGarragh Gregory , Natraj Vijay
Published: July 2022

The Orbiting Carbon Observatories-2 and -3 make space-based measurements in the oxygen A-band and the weak and strong carbon dioxide (CO2) bands using the Atmospheric Carbon Observations from Space (ACOS) retrieval. Within ACOS, a Bayesian optimal estimation approach is employed to retrieve the column-averaged CO2 dry air mole fraction from these measurements. This retrieval requires a large number of polarized, multiple-scattering radiative transfer calculations for each iteration. These calculations take up the majority of the processing time for each retrieval and slow down the algorithm to the point that reprocessing data from the mission over multiple years becomes especially time consuming. To accelerate the radiative transfer model and, thereby, ease this bottleneck, we have developed a novel approach that enables modeling of the full spectra for the three OCO-2/3 instrument bands from radiances calculated at a small subset of monochromatic wavelengths. This allows for a reduction of the number of monochromatic calculations by a factor of 10, which can be achieved with radiance errors of less than 0.01% with respect to the existing algorithm and is easily tunable to a desired accuracy-speed trade-off. For the ACOS retrieval, this speeds up the over-retrievals by about a factor of two. The technique may be applicable to similar retrieval algorithms for other greenhouse gas sensors with large data volumes, such as GeoCarb, GOSAT-3, and CO2M.
Developing and Maintaining Sport-Confidence: Learning From Elite Athletes
Authors: Thomas Owen , Thrower Sam N.
Published: July 2022

Have you noticed how you can feel very confident in some sporting situations but in others, usually at the worst possible moment, that confidence can suddenly disappear? When athletes feel confident, they are focused on the task, feel relaxed, and commit fully to decisions—all of which help them perform well. However, when they do not feel confident, they sometimes focus on the wrong things, doubt themselves, feel nervous, and often make poor decisions, which often results in poorer performances. In this article, we talk about a study that investigated what young elite athletes feel confident about, where they get their confidence from, and what reduces their confidence. The results help to explain why the confidence of young athletes often fluctuates and, importantly, gives us clues about how to develop and maintain sport-confidence.
Effect of Molybdenum Content on Microstructure and Mechanical Properties of Ti-Mo-Fe Alloys by Powder Metallurgy
Authors: Hwang Hyo-Woon , Park Ji-Hwan , Lee Dong-Geun
Published: July 2022

Titanium has many limitations in coverage and frequency of application due to its expensive alloying elements and complex manufacturing process. The biocompatible Ti-Mo-Fe ternary beta titanium alloys were designed by replacing high-cost beta-stabilizer elements (V, Nb, Zr, etc.) with low-cost Mo and Fe elements. In addition, it was attempted to obtain a low-cost, high-strength beta-titanium alloy with 800 MPa or more by applying the powder metallurgy process technology to the Ti-Mo-Fe alloy system. The added Mo element has the effect of reducing the elastic modulus of the titanium alloy without reducing its strength. In this study, Ti-Mo-Fe alloys designed with different Mo contents were fabricated using a powder metallurgy process and analyzed in connection with microstructural properties, phase changes, and mechanical properties. As Mo contents are increased, the α-lath thickness of Widmanstätten decreases and the size of prior β grain decreases. It was confirmed that the hardness and tensile strength were excellent and were compared with the ingot material of the same alloy system.
Comparison of Measured and Observed Exercise Fidelity during a Neuromuscular Training Warm-Up
Authors: Benson Lauren C. , Räisänen Anu M. , Sidhu Sartaj S. , Emery Carolyn A.
Published: July 2022

Neuromuscular training (NMT) warm-up programs effectively prevent injuries in youth, but monitoring exercise fidelity is challenging. The purpose of this study was to compare the exercise fidelity as measured via an inertial measurement unit (IMU) with direct observations of selected exercises. Youth basketball and soccer players performed single leg jumps, squat jumps, Nordic hamstring curls, and/or single leg balance exercises as part of an NMT warm-up. An IMU was placed on the lower back of each participant and the warm-up was video recorded. A physiotherapist evaluated the volume aspect of exercise fidelity (i.e., performing the prescribed number of repetitions) using the video recordings and a checklist. Algorithms were developed to count the number of repetitions from the IMU signal. The repetitions from the algorithms were compared with the physiotherapist’s evaluation, and accuracy, precision, and recall were calculated for each exercise. A total of 91 (39 female, 52 male) athletes performed at least one of the four warm-up exercises. There was an accuracy, precision, and recall of greater than 88% for all exercises. The single leg jump algorithm classified all sets correctly. IMUs may be used to quantify exercise volume for exercises that involve both impact during landing and changes in orientation during rotations.
Students’ Perception towards New Face of Education during This Unprecedented Phase of COVID-19 Outbreak: An Empirical Study of Higher Educational Institutions in Saudi Arabia
Authors: Asif Mohammad , Khan Mohammed Arshad , Habib Sufyan
Published: July 2022

Purpose: To examine the perception of students regarding an e-learning system adopted by various educational institutions in the Kingdom of Saudi Arabia during the COVID-19 pandemic. Methodology: A web-based-survey was conducted among selected university students in Saudi Arabia. A total of 294 students were randomly chosen to determine the utilities and credibility of the adopted e-learning mode of education. The reliability of latent constructs was assessed according to Cronbach’s alpha, and confirmatory factors analysis was conducted via AMOS software (version 24) to measure the students’ perceptions of online learning. Results: The outcomes of the present study reveal that e-learning has been very useful throughout the pandemic period among selected Saudi Arabian universities. The students have a positive view of the online system of education, which has many benefits, including flexibility, low cost, self-learning, and convenience. Implications: The results of the present study will be beneficial for all educational institutions that are largely dependent on the findings of the online survey.
A New 7 kW Air-Core Transformer at 1.5 MHz for Embedded Isolated DC/DC Application
Authors: Rigot Valentin , Phulpin Tanguy , Sakly Jihen , Sadarnac Daniel
Published: July 2022

This paper presents the study of air-core transformers for electric vehicles, developing them for medium-power (tens of kWs) converter applications specifically used at a high frequency. Air-core transformers have the advantage of lacking magnetic saturation and iron losses, making them suitable for high-frequency applications. We designed and manufactured a transformer for a determined frequency and inductance value. The design of this passive component aims to both keep the magnetic field inside the transformer and manage the thermal energy efficiently. The electrical, magnetic, and thermal properties are simulated and then verified by experiments with a specific test bench. The transformer reaches high performances for a higher frequency than usual for an equivalent power transfer in automotive applications.
A Study on the Safety Analysis of an Inductive Power Transfer System for Kitchen Appliances
Authors: Liu Ying , Zhang Jiantao , Zhu Chunbo , Chan Ching Chuen
Published: July 2022

This paper presents a detailed analysis of the safety of human bodies in the electromagnetic field generated by inductive power transfer (IPT) systems designed for kitchen appliances. Comparisons of basic and reference limit values of various safety standards are investigated through theoretical circuit analysis and extensive simulation studies. Simulation models of human bodies along with an IPT system for kitchen appliances are established to reveal the effect of the electromagnetic field on the human body. Corresponding experiments are conducted via constructing a configuration of the designed IPT system and simulating the standing position. Both experimental and analytical results indicate that it is easier to fulfill international safety standards by increasing the operating frequency of the IPT system for kitchen appliances, and hence, the safety of human bodies can be effectively improved.
Pressurized Chemical Looping for Direct Reduced Iron Production: Carbon Neutral Process Configuration and Performance
Authors: Bond Nicole , Symonds Robert , Hughes Robin
Published: July 2022

To achieve net-zero iron and steel production by 2050, many iron and steel producers are turning to direct reduced iron (DRI)—electric arc furnace (EAF) steel production as an opportunity to achieve significant CO2 emissions reductions relative to current levels. However, additional innovations are required to close the gap between DRI and net-zero steel. Pressurized chemical looping-DRI (PCL-DRI) is a novel technology explored to meet this target, in which the reformer firebox and fired process gas heaters are replaced with PCL combustion units. Captured CO2 is conditioned and compressed for pipeline transportation and storage/utilization. The performance of two different PCL-DRI configurations relative to traditional DRI processes was explored via process simulation: a Midrex-type process and an Energiron-type process. The PCL-DRI processes were shown to have equivalent or lesser total fuel consumption (8% reduction) compared to the base cases, and greater process water production (170–260% increase), with minimal or no loss in thermal efficiency. PCL-DRI is a strong competitor to alternative methods of reaching net-zero DRI due to lower energy penalties for carbon capture, no required changes to stream chemistry in or out of the EAF, and no requirement for hydrogen infrastructure.
Research on Vehicle-to-Vehicle MIMO Wireless Channels in Various Tunnels
Authors: Zhou Jie , Wu Sujie , Lv Zhikang , Luo Hong , Liu Ting , Shao Genfu
Published: July 2022

In this paper, we propose a geometry-based channel model for multiple-input multiple-output (MIMO) vehicle-to-vehicle (V2V) communications in practical tunnel scenarios. In the proposed channel model, the waves transmitted from the transmitter experience line-of-sight (LoS) and non-LoS (NLoS) before arriving at the receiver. The time-varying parameters of the propagation paths and angles are derived to characterize the channel non-stationarity. Furthermore, we derive the correlation property functions of the proposed V2V channel model; they are space cross-correlation functions (CCFs), temporal auto-correlation functions (ACFs), frequency CCFs, as well as the Doppler power spectrum distributions (PSDs). Numerous simulation results of the propagation characteristic for different physical properties of the tunnel, as well as the different motion properties of the transmitter and receiver, are studied and discussed; they demonstrate that the proposed channel model is suitable for describing the practical V2V tunnel communication scenarios.
Energy Anxiety in Decentralized Electricity Markets: A Critical Review on EV Models
Authors: Gopinathan Nandan , Shanmugam Prabhakar
Published: July 2022

The automobile sector is a promising avenue for enhancing energy security, economic opportunity, and air quality in India. Before penetrating a large number of electric vehicles (EV) into the power grid, a thorough investigation and assessment of significant parameters are required, as additional nonlinear and EV loads are linked to the decentralized market. Many automobile companies have already invested in electric vehicle research; hence, a detailed analysis on range anxiety and grid connectivity concerns are the important factors affecting the future of the electric vehicle industry. In this paper, the initial review is about the decentralized market in India and sustainable aspects of electric mobility based on the Indian context, as it is a developing nation with an enormous resource and scope for EV markets. With recent literature from the last three years, the substantial constraints observed in benefits and challenges are reviewed. The financial stability aspects and the incentives to overcome the barriers to EV adoption are briefly discussed. From the review, it has come to the limelight that infrastructure availability, technology, load demand, and consumer behaviour are all major obstacles in the electric vehicle ecosystem. For the overall design and study of the vehicle to grid (V2G) infrastructure, this paper also provides insight into the representation of electric vehicles in different energy-efficient models and their categorization while connecting to the grid. The methodology adopted for energy-efficient models includes lifecycle emissions, economy, smart charging, real-time optimization, aggregated EV resource modelling, and a support vector machine (SVM)-based method. This paper gives a positive impact on EV fleet integration and electric mobility in general, as it critically reviews the influential parameters and challenges. This classification depends on crucial parameters that are at the frontline of EV grid integration research. This review is a solution to enhance grid stability in regard to new EV models. With the advanced electric motors development and renewed battery technology models, longer-distance automobiles are now available on the market. This paper investigates the constraints of EV grid integration and analyzes different EV models to ease the grid stability for a decentralized market.
Volume of Interest-Based Fractal Analysis of Huffaz’s Brain
Authors: Jamaludin Iqbal , Che Azemin Mohd Zulfaezal , Mohd Tamrin Mohd Izzuddin , Sapuan Abdul Halim
Published: July 2022

The robust process in memorising the Quran is expected to cause neuroplasticity changes in the brain. To date, the analysis of neuroplasticity is limited in binary images because greyscale analysis requires the usage of more robust processing techniques. This research work aims to explore and characterise the complexity of textual memorisation brain structures using fractal analysis between huffaz and non-huffaz applying global box-counting, global Fourier fractal dimension (FFD), and volume of interest (VOI)-based analysis. The study recruited 47 participants from IIUM Kuantan Campus. The huffaz group had their 18 months of systematic memorisation training. The brain images were acquired by using MRI. Global box-counting and FFD analysis were conducted on the brain. Magnetic resonance imaging (MRI) found no significant statistical difference between brains of huffaz and non-huffaz. VOI-based analysis found nine significant areas: two for box-counting analysis (angular gyrus and medial temporal gyrus), six for FFD analysis (BA20, BA30, anterior cingulate, fusiform gyrus, inferior temporal gyrus, and frontal lobe), and only a single area (BA33) showed significant volume differences between huffaz and non-huffaz. The results have highlighted the sensitivity of VOI-based analysis because of its local nature, as compared to the global analysis by box-counting and FFD.
Publisher’s Note: Future—A New Open Access Journal
Authors: Stefenelli Giulia
Published: July 2022

New generations are key players at present, but they are also our future, those who will lead any future changes and innovation [...]
Testis-Specific Isoform of Na+-K+ ATPase and Regulation of Bull Fertility
Authors: Tiwari Saurabh , Rajamanickam Gayathri , Unnikrishnan Veena , Ojaghi Mina , Kastelic John P. , Thundathil Jacob C.
Published: July 2022

An advanced understanding of sperm function is relevant for evidence-based male fertility prediction and addressing male infertility. A standard breeding soundness evaluation (BSE) merely identifies gross abnormalities in bulls, whereas selection based on single nucleotide polymorphisms and genomic estimated breeding values overlooks sub-microscopic differences in sperm. Molecular tools are important for validating genomic selection and advancing knowledge on the regulation of male fertility at an interdisciplinary level. Therefore, research in this field is now focused on developing a combination of in vitro sperm function tests and identifying biomarkers such as sperm proteins with critical roles in fertility. The Na+-K+ ATPase is a ubiquitous transmembrane protein and its α4 isoform (ATP1A4) is exclusively expressed in germ cells and sperm. Furthermore, ATP1A4 is essential for male fertility, as it interacts with signaling molecules in both raft and non-raft fractions of the sperm plasma membrane to regulate capacitation-associated signaling, hyperactivation, sperm-oocyte interactions, and activation. Interestingly, ATP1A4 activity and expression increase during capacitation, challenging the widely accepted dogma of sperm translational quiescence. This review discusses the literature on the role of ATP1A4 during capacitation and fertilization events and its prospective use in improving male fertility prediction.
Nanoparticle Effects on Stress Response Pathways and Nanoparticle–Protein Interactions
Authors: Cameron Shana J. , Sheng Jessica , Hosseinian Farah , Willmore William G.
Published: July 2022

Nanoparticles (NPs) are increasingly used in a wide variety of applications and products; however, NPs may affect stress response pathways and interact with proteins in biological systems. This review article will provide an overview of the beneficial and detrimental effects of NPs on stress response pathways with a focus on NP–protein interactions. Depending upon the particular NP, experimental model system, and dose and exposure conditions, the introduction of NPs may have either positive or negative effects. Cellular processes such as the development of oxidative stress, the initiation of the inflammatory response, mitochondrial function, detoxification, and alterations to signaling pathways are all affected by the introduction of NPs. In terms of tissue-specific effects, the local microenvironment can have a profound effect on whether an NP is beneficial or harmful to cells. Interactions of NPs with metal-binding proteins (zinc, copper, iron and calcium) affect both their structure and function. This review will provide insights into the current knowledge of protein-based nanotoxicology and closely examines the targets of specific NPs.