bluuebunny/crossref_metadata_2025 huggingface dataset:
Vitamin B2 content determination in liver paste by using acid and acid-enzyme hydrolysis
Authors: Basic Zorica , Kilibarda Vesna , Miletic Ivanka
Published: December 2010

Background/Aim. Vitamin B2 is available in foodstuff in the form of coenzyme and in free form. For its content determination a few procedures should be performed (deliberation from a complex, extraction of free and deliberated form) and detection, identification and quantification. There is a particular problem in determination of vitamin B2 in the meat products. For a determination of total vitamin B2 content in liver paste two preparation procedures are compared: acid and acid-enzymatic hydrolysis. The aim of this study thus, was to compare the effectiveness of these two different procedures for vitamin B2 content determination in liver paste. Methods. High pressure liquid chromatography (HPLC) method with fluorescence detector, as specific and adequately sensitive for the foodstuff of a complex composition with a natural vitamin content, was used for determination of vitamin B2 in liver paste. Acid hydrolysis was performed with the application 0.1 M hydrochloric acid in a pressure cooker, and enzymatic hydrolysis was performed with the 10% takadiastase on 45 ?C within four hours. Ten samples of liver paste from the supply of the Serbian Army were examined. Separation was performed on the analytical column Nucleosil 50?5 C18 with mobile phase 450 ml CH3OH + 20 ml 5 mM CH3COONH4, and detection on the fluorescent detector with the variable wave length. Both methods were validated: examining a detection limit, quantification limit, specificity (because of a possible B2 vitamin interference with reagents), linearity of a peak area and standard concentration of B2 vitamin ratio in the range from 0.05 ?g/ml to 2 ?g/ml, precision for the 0.05 ?g/ml concentration and recovery. Results. All the previously examined parameters validated both methods as specific, precise and reproductive, with a high recovery (98.5% for acid and 98.2% for acid - enzymatic hydrolysis), as well as linearity in a range that significantly superseded the expected content in the samples (r = 0.9994, and r = 0.99987). Hydrolysis procedures make a sample suitable for vitamin B2 determination. In the liver paste samples a high content of vitamin B2 was determined: 0.83 mg/100 g after acid hydrolysis, and 0.909 mg/100 g after acid-enzyme hydrolysis. There were statistically significantly higher values determined after the acid-enzyme hydrolysis (p < 0.05). Conclusion. Using acid-enzyme hydrolysis and separation instrument technique (liquid chromatography) with a fluorescent detector as detection system, statistically significantly greater vitamin B2 quantities were determined than after using acid hydrolysis procedure. Vitamin B2 content determined in ten liver paste samples was high (0.881 ? 0.936 mg/100g) indicating that this meat product is a good vitamin B2 source.
Risk factors for the appearance of central venous catheters colonization
Authors: Mioljevic Vesna , Suljagic Vesna , Jovanovic Biljana , Gligorijevic Jelena , Jovanovic Snezana , Mazic Natasa
Published: December 2010

Introduction/Aim. Intravascular device placement (IVD) is a part of everyday medical practice, however, its application is associated with a high risk of onset of nosocomial infections (NI) and increased mortality and morbidity. Nosocomial blood infections (NBIs) account for 10% of all the registered NI. NBIs are more frequent in patients with a placed IVD and it present an important risk factor for the onset of NBI, i.e. catheter-associated NBIs (CANBIs). Pathogenesis of CANBIs is complex and conditioned by the presence of different characteristics related to a catheter, patient and a specific causative organism. The most common CRBSI causes include coagulase-negative staphylococcus, S. aureus, Enterobacter spp, Candida spp, Klebsiella spp, Pseudomonas spp. and Enterococcus spp. Methods. All the patients hospitalized at the Intensive Care Department of the Clinic of Digestive Diseases over the period January 1, 2004-September 1, 2004 were retrospectively analyzed. The study included 107 patients in whom central venous catheter (CVC) was placed for more than 48 h. All the causes isolated from a CVC segment were recorded. Culture, isolation and identification of the causative organisms were performed using standard microbiological methods in the Bacteriological Laboratory within the Emergency Center, Clinical Center of Serbia. Catheter segment samples (tip of the CVC 3-5 cm long) were analyzed. Based on the insight into medical documentation, patients? examination and medical staff interview, catheter and patient-related characteristics were recorded. Results. A total of 107 CVCs were analyzed, out of which 56 (52%) were sterile while 51 (48%) were colonized. The results of our study evidenced that total parenteral nutrition (TPN) (p < 0.05), number of catheterization days (p < 0.05), and central venous pressure measurement (p < 0.05) were significantly associated with CVC colonization. In this study, no statistically significant difference in catheter colonization was found with respect to sex, age, anatomical insertion site and CVC placement site. Conclusion. According to the results of our study, TPN, the number of catheterization days and measurement of central venous pressure play major roles in colonization of CVC. Understanding risk factors associated with CVC colonization and onset of CANBIs is a prerequisite for quality preventive work of health professionals.
Treatment of hepatorenal syndrome
Authors: Kezic Aleksandra , Mijac Dragana , Stojimirovic Biljana
Published: December 2010

 Hepatorenalni sindrom je funkcionalna insuficijencija bubrega u sklopu terminalne insuficijencije jetre. U patogenezi ovog sindroma ucestvuju hemodinamske promene (snizeni srednji arterijski pritisak i perfuzioni pritisak bubrega) i povecano stvaranje sistemskih i bubreznih vazoaktivnih medijatora sa stimulacijom bubreznog simpatickog sistema. Standardna medicinska farmakoloska terapija usmerena je na prethodno navedene patofizioloske poremecaje. U poslednje vreme postupci detoksikacije, npr. jedan od vidova albuminske dijalize, pojavljuju se sve vise u lecenju bolesnika sa HRS, ali se jos ne preporucuju kao standardna procedura. Oba vida lecenja preduzimaju se samo kao premoscenje perioda do transplantacije jetre ili u slucajevima kada se ocekuje oporavak funkcije jetre, jer bubrezna funkcija direktno zavisi od funkcije jetre. Standardna hemodijaliza ne pomaze u poboljsanju funkcije bubrega. Jedino kauzalno resenje ovog problema donosi transplantacija jetre.
The influence of Helicobacter pylori infection on the occurrence of gastroesophageal reflux in patients with renal insufficiency
Authors: Stolic Radojica , Jovanovic Aleksandar , Peric Vladan , Trajkovic Goran , Zivic Ziva , Stolic Dragica , Lazarevic Tatjana , Sovtic Sasa
Published: December 2010

Introduction/Aim. Gastric acid is a key factor in the pathophysiology of gastroesophageal reflux disease. A plausible mechanism by which the Helicobacter pylori infection might protect against reflux disease is by its propensity to produce atrophic gastritis. The aim of the study was to establish the influence of Helicobacter pylori infection on the occurrence of gastroesophageal reflux in patients with different stages of renal insufficiency. Methods. The examination was organized as a prospective, clinical study and involved 68 patients ? 33 patients with preterminal stage of renal failure and 35 patients with terminal renal insufficiency. Due to dyspeptic difficulties, in all the patients there was preformed upper esophagogastroscopy and Helicobacter pylori infection was found by ureasa test. Results. The patients with preterminal renal insufficiency were significantly younger than patients with terminal renal failure (53.4?11.1 vs. 65.4?12.3 years; p = 0.014). There was found a statistically significant difference between the groups in Helicobacter pylori infection (p = 0.03), hiatal hernia (p = 0.008), gastroesophageal reflux disease (p = 0.007), and duodenal ulcer (p = 0.002). Using the multiple non-parametric correlative analysis there was confirmed a negative correlation between Helicobacter pylori infection and gastro-esophageal reflux disease (Kendal ?B = -0.523; p = 0.003) and hiatal hernia (Kendal ?B = 0.403; p = 0.021), while there was found a positive correlation between gastro-esophageal reflux disease and hiatal hernia (Kendal ?B = 0.350; p = 0.044). Conclusion. Helicobacter pylori infection is a significant protective parameter of the incidence of gastro-esophageal reflux disease in patients with both pre-terminal and terminal renal insufficiency.
Influence of age, corneal astigmatism and some characteristics of intraocular lens on glare in pseudophakic persons
Authors: Avramovic Sinisa , Vukosavljevic Miroslav
Published: December 2010

Background/Aim. Although an extraordinary visual acuity is achieved following the extracapsular cataract extraction with the implantation of intraocular lens, all the problems of vision are not solved in so doing and many of them should be considered. The aim of this study was to establish the influence of age, dioptric power of intraocular lens, corneal astigmatism and optic diameter of intraocular lens on glare in pseudophakic subjects. Methods. We examined 152 patients who underwent final correction at least two weeks after the suture removing what was performed if the astigmatism more than 2.0 D was measured by the use of a Javal keratometer. Examination was performed by a Rodenstock Adaptometer Nyctomat. According to the recommendation of the manufacturer, healthy persons should notice 50% of contrast under the constant glare and in the given time. Statistical data processing was performed by ?2 test. Results. In the group of patients with the findings lower than normal at constant glare there were 112 of eyes (73.68%) as follows: 22 eyes (14.47%) at the age from 50-55 years; 15 eyes (9.87%) were at the age from 56-60 years; 18 eyes (11.84%) were at the age from 61-65 years, and 57 eyes (37.50%) were at the age from 66-70 years. Dioptric power of lenses up to 20.0 D had 37 eyes (24.3%), from 20.5-22.0 D 85 eyes (56.9%) and more than 22.5 D, 27 eyes (17.8%). Regarding the results of discomfort caused by glare and corneal astigmatism, measured by a Javal keratometer, in the observed group normal results were found in 112 eyes (73.68%), and out of that number 95 (62.50%) were with corneal astigmatism less than 20.0 D, and 17 (11.18%) had astigmatism of 20.0 D and more. Out of 112 eyes there were 58 of them (38.16%) with findings lower than normal in relation to the constant glare and with the lens diameter of 5.5 mm, 48 (31.58%) with the lens diameter of 6.0mm, and 6 of them (3.95%) with lens diameter of 6.5mm. Conclusion. The study determined a significant statistical correlation between the dioptric power of lenses and the glare. The obtained results did not find out a significant increase in glare in relation to the age of subjects, optic diameter and corneal astigmatism.
Low-power laser therapy in the treatment of parodontopathy
Authors: Pejcic Ana , Grujicic Dragan
Published: December 2010

 Zahvaljujuci opisanim terapijskim efektima, laseri male snage deluju na biolosko tkivo, prvenstveno antiinflamacijski, ali i stimulativno na reparacijske i regeneracijske procese u inflamiranoj gingivi. Zbog toga se laseri danas primenjuju kao dopunska, veoma uspesna, fizikalna metoda lecenja, koja, uz tradicionalnu periodontalnu terapiju, daje bolje rezultate lecenja i utice na duze odrzavanje postignutih terapijskih rezultata.
Early predictors of left ventricular function improvement late after myocardial infarction
Authors: Mladenovic Zorica , Angelkov-Ristic Andjelka , Kalimanovska-Ostric Dimitra , Mijailovic Zdravko , Gligic Branko , Obradovic Slobodan , Tavciovski Dragan , Jovelic Aleksandra
Published: December 2010

Background/Aim. Prognosis after acute myocardial infarction (AIM) depends on the extent of irreversibly damaged myocardium and viable tissue due to stunning or hibernation. The aim of the study was to assess the prognostic significance of early echocardiographic parameters of myocardial viability in prediction of late recovery of regional and global ventricular function. Methods. The study prospectively included 40 patients after the first, uncomplicated univessel AIM treated with percutaneous coronary intervention (PCI). Low-dose dobutamine echocardiography (LDDE) was preformed 7-10 days after AIM and follow-up resting echocardiography from 7 to 12 months later. Results. The sensitivity and specificity for the prediction of post revascularisation regional, dyssynergy improvement were 61.29% and 94.59% respectively. The positive and negative predicative values were 90.48% and 74.47% respectively. The number of viable segments (p = 0.01) and extent of contractile reserve (p = 0.01) were univariate, independent predictors of improvement in ejection fraction (EF). From the multivariate stepwise regression analysis contractile reserve was selected as most powerful predictor of late recovery of left ventricular contractile function (p = 0.007). Receiving-operator characteristic curve (ROC) analysis demonstrated that three or more recovered segments were necessary for an improvement of left ventricular ejection fraction (LVEF) ? 5% after the revascularisation, with the highest sensitivity, 100% and specificity 56% (p = 0.01). Conclusion. Low-dose dobutamine echocardiography is a powerful predictor of the regional dyssynergy recovery late after AIM treated with PTCA with implantation stent. Late full functional improvement of the left ventricle is related to the extent of contractile reserve and amount of viable tissue. At least three recovered segments are necessary for a significant recovery of the global left ventricular contractility.
Surgical treatment of lung sequestration
Authors: Cvijanovic Vlado , Stanic Vojkan , Ristanovic Aleksandar , Gulic Bojan , Stamenovic Davor , Maric Nebojsa , Kovacevic Snezana , Zolatarevski Lidija
Published: December 2010

Background/Aim. Pulmonary sequestration is a congenital malformation which consists of a functional part of the lung, separated of the normal airway, and vascularisated with anomal systemic artery instead of pulmonary artery. There are two kinds of sequestration. Intralobar is surrounded with normal lung and its pleura, and extralobar which has extrapulmonary position and pleura of its own. This anomaly is very rare and appears in 1.1?1.8% of all congenital lung malformations. The illness is revealed either in early childhood with other life-threatening anomalies or in adulthood and middle age when secondary infection arises. The aim of this paper was to show our own experience in surgical treatment of pulmonary sequestration and to emphasize sequestration as a real differential-diagnostic possibility with patients with recidive bronchopneumonias. Methods. We retrospectively analyzed medical records for the period from 1967?2007 and found 15 patients with pulmonary sequestration at the average age of 30 years. We pointed out the well known problems with identification of this anomaly, preoperative diagnostic procedures and surgical possibilities of treatment. Results. There were 13 patients with intralobar and two patients with extralobar sequestration. By the use of preoperative angiography, seven patients were found to have intralobar pulmonary sequestration. All intralobar sequestrations were clinically manifested, the most often with recidive bronchopneumonia. Six patients had no preoperative diagnosis of lung sequestration. The most common locality of intralobar sequestration was the left lower lobe (eight patients). We performed nine lobectomies, three sequestrectomies, two segmentectomies and one pneumonectomy. Both extralobar sequestrations were diagnosed intraoperatively. Conclusion. Pulmonary sequestration is a rare malformation. Diagnosis is established by angiography. Treatment is exclusively surgical. In the last three years we have had one patient per year. This experience obliges to consider pulmonary sequestration as a real differential- diagnostic possibility in patients with localized repeated bronchopneumonias.
Application possibilities of osteodensitometry in detecting and preventing periprosthetic bone resorption following cementless total hip artheroplasty
Authors: Starcevic Srdjan , Popovic Zoran , Petronijevic Milan
Published: December 2010

 Najcesca komplikacija ugradnje bescementne endoproteze kuka kod bolesnika sa koksartrozom jeste periproteticna osteoresorpcija koja se javlja u toku prvih nedelja i meseci posle ugradnje implantata i koja dovodi do njegovog labavljenja ili periproteticnog preloma butne kosti. Osteodenzitometrijskim merenjem BMD pre ugradnje endoproteze, a zatim kontinuiranim pracenjem promena BMD posle operacije, moguce je otkriti bolesnike koji imaju povecan rizik od razvoja masivne periproteticne osteoresorpcije i pravovremeno preduzeti neophodne mere za obezbedjenje dugotrajne stabilnosti endoproteze, a time i kvaliteta zivota bolesnika sa koksartrozom.
Radical resection of giant chondrosarcoma of the anterior chest wall
Authors: Stanic Vojkan , Vulovic Tatjana , Novakovic Marjan , Ristanovic Aleksandar , Stamenovic Davor , Cvijanovic Vlado , Stepic Nenad , Djordjevic Gordana
Published: December 2010

Background. Chondrosarcomas represent approximately 30% of primary malignant bone tumors, the most frequent of which is on anterior thoracic wall. Case report. We presented a case of 50-year-old man suffering from a slowgrowing, painless giant chondrosarcoma of the anterior chest wall. A wide resection was performed to excise the tumor including attached skin, right breast, ribs, sternum, soft tissues and parietal pleura. Mediastinum was not affected by the tumor. After resecting a 26 ? 20 ? 22 cm segment, the chest wall defect was reconstructed with a Marlex mesh and extensive latissimus dorsi myocutaneous flap pedicled on the right thoracodorsal vessels. Histopatology diagnosis was chondrosarcoma G 2?3. The mechanics of ventilation was not altered and respiratory function was normal from the immediate postoperative period. Three years after the operation postoperative results showed no local recurrence and excellent functional and aesthetic results were evident. Respiratory function remained unaltered. Conclusion. According to the results it can be concluded that the use of Marlex mash and myocutaneous flap is good method for stabilization of the chest wall and enough to avoid paradoxical respiratory movements in managing giant chondrosarcoma of the anterior chest wall.
Diastolic dysfunction types in the prediction of viable myocardium functional recovery
Authors: Matunovic Radomir , Mijailovic Zdravko , Tavciovski Dragan , Cosic Zoran , Stajic Zoran
Published: December 2010

Background/Aim. It is well known that patients with coronary artery disease and viable tissue as a guarantee of contractile recovery (CR), despite of decreasing ejection fraction (EF) and systolic dysfunction, could have benefit from surgical revascularization. Therefore, relationship between diastolic filling type and early postoperative recovery and complications need to be established. The aim of this study was to investigate the relation between different left ventricular (LV) diastolic filling types and CR in patients after surgical revascularization with differently preserved systolic function. Methods. We investigated 60 patients. All of them had CR estimated by stress echocardiography regardless the extent of recovery of the heart systolic function. Echocardiographic evidence of diastolic dysfunction was estimated by Doppler examination of transmitral diastolic flow. According to the derived different diastolic filling types the patients were divided into three groups: I ? patients with disorder of LV relaxation, II ? with pseudovascularisation, and III ? with restrictive filling type, and according to the value of systolic function into two subgroups: 1) relatively recovered systolic function ? EF > 40% and 2) pronounced LV dysfunction ? EF < 40%. Echocardiographic evaluation was performed before and two week after surgical revascularization. In the preoperative period the medication therapy was optimized. We estimated CR by echocardiografic paremeters but also by detection of cardiovascular events. Results. After CABG the mean value of WMSI LV tended to decrease in any groups: in the group I (n = 12) from 1.64?0.22 to 1.34?0.22; in the group II (n = 22) from 1.85?0.16 to 1.53?0.42, and in the group III (n = 26) from 1.92?0.29 to 1.81?0.52. The lowest improvement of systolic function according to EF value expressed by the number of patients was found in the group of patients with restrictive LV filling type (12; 53.8%) as contrasting to the group with pseudonormalisation (15; 78.9%). In the group of patients with restrictive diastolic filling type also was recorded the highest number of lethal outcomes (6; 23.1%), as well as cardiovascular complications (10; 38.5%). Conclusions. Restrictive LV diastolic filling type was the marker of poor prognosis in the patients with clinical heart failure undergoing surgical revascularization. The patients with heart failure and preserved systolic function were associated with similar prognosis.
Ambulatory arterial blood pressure monitoring in patients before and after thyroidectomy
Authors: Ivanovic Branislava , Paunovic Ivan , Nikcevic Djordje , Cvijanovic Dane , Kalezic Nevena , Simic Dragan
Published: December 2010

Bacground/Aim. Increased values of thyroid hormones in the clinical syndrome of hyperthyreosis affect blood pressure values and its circadial variation. The aim of this study was to define the influence of hyperthyreosis on the values and circadial variations of arterial blood pressure, as well as to investigate the effect of thyroid surgery on blood pressure values. Methods. We compared the 24-hour averages of systolic and diastolic blood pressure, their variations and their reduction during the night between 20 female patients with hyperthyroidism and hypertension de novo and 20 healthy females. We compared the values of 24-hour ambulatory monitoring performed before the surgery with the values gathered two weeks after the surgery. Results. The 24-hour average systolic and diastolic blood pressure values were higher in the patients with hyperthyroidism than in the control group (p < 0.001). In the group of patients, the variations in blood pressure were significantly higher than they were in the group of healthy people (p < 0.001). The amplitude of the nocturnal reduction of blood pressure was also significantly lower in the patients with hyperthyroid status and hypertension, in comparison to the healthy persons (p < 0.001). Two weeks after the surgery, a significant reduction of blood pressure values (both for systolic and diastolic) appeared. Conclusion. The patients with hyperthyroidism- caused hypertension had higher systolic and diastolic blood pressure, higher variations in blood pressure and lower nocturnal reduction of blood pressure than healthy subjects. Thyroid surgery, as a control of thyroid function, optimised blood pressure very rapidly.
Correlation of dental age and anthropometric parametres of the overall growth and development in children
Authors: Trickovic-Janjic Olivera , Apostolovic Mirjana , Janosevic Mirjana , Filipovic Gordana
Published: December 2010

Introduction/Aim. Anthropometric methods of measuring the whole body and body parts are the most commonly applied methods of analyzing the growth and development of children. Anthropometric measures are interconnected, so that with growth and development the change of one of the parameters causes the change of the other. The aim of the paper was to analyse whether dental development follows the overall growth and development and what the ratio of this interdependence is. Methods. The research involved a sample of 134 participants, aged between 6 and 8 years. Dental age was determined as the average of the sum of existing permanent teeth from the participants aged 6, 7 and 8. With the aim of analyzing physical growth and development, commonly accepted anthropometric indexes were applied: height, weight, circumference of the head, the chest cavity at its widest point, the upper arm, the abdomen, the thigh and thickness of the epidermis. The dimensions were measured according to the methodology of the International Biological Programme. The influence of the pertinent variables' related size on the analyzed variable was determined by the statistical method of multivariable regression. Results. The middle values of all the anthropometric parametres, except for the thickness of the epidermis, were slightly bigger with male participants, and the circumference of the chest cavity was statistically considerably bigger (p < 0.05). The results of anthropometric measurement showed in general a distinct homogeneity not only of the sample group but also within gender, in relation to all the dimensions, excyt for the thickness of the epidermis. The average of the dental age of the participants was 10.36, (10.42 and 10.31 for females and males respectively). Considerable correlation (R = 0.59) with high statistical significance (p < 0.001) was determined between dental age and the set of anthropometric parameters of general growth and development. Conclusion. There is a considerable positive correlation (R = 0.59) between dental age and anthropometric parameters of general growth and development, which confirms that dental development follows the overall growth and development of children, aged between 6 and 8 years.
Frequency and characteristics of metabolic disorders in patients on haemodialysis
Authors: Stolic Radojica , Trajkovic Goran , Peric Vladan , Jovanovic Aleksandar , Stolic Dragica , Sovtic Sasa , Subaric-Gorgieva Gordana
Published: December 2010

Background/Aim. Metabolic syndrome and malnutrition represent metabolic abnormalities which significantly characterize patients on haemodialysis. The aim of the study was to determine the incidence and find characteristics of metabolic disbalance in patients on haemodialysis. Methods. The study involved 124 patients on chronic haemodialysis at the Clinical Centre Kragujevac. There were analyzed demographic and anthropometric characteristics of the examined patients. Of clinical characteristics, there were determined smoking habit, time on dialysis, arterial pressure; of comorbide states there were recorded heart diseases and diabetes mellitus. Routine biochemical analyses were carried out by a standard laboratory procedure. Results. Metabolic syndrome was found in 29.8% of the examined patients. The patients with metabolic syndrome were older as compared to the patients without metabolic syndrome (58.45?12.91 vs 52.25?11.63 years). The values of systolic pressure (143.88?19.75 vs 133.01?22.93 mmHg; p = 0.014), body mass index (21.2?3.71 vs 19.4?2.88 kg/m2; p = 0.001), fat body mass (19.57?8.47 vs 16.45?5.82%; p = 0.0002) and waist scope (89?12.54 vs 96?12.34 cm; p = 0.0001) were significantly higher in the patients with metabolic syndrome as compared to those without metabolic syndrome. The values of erythrocytes (3.4?0.45 vs 19?0.53 ? 1012; p = 0.04) and hemoglobin (107?15.76 vs 101?13.87 g/l; p = 0.009), glycaemia (9.5?8.15 vs 5.6?1.4 mmol/l; p = 0.04) triglycerides (2.44?1.8 vs 1.41?0.64 mmol/l; p = 0.007), HDL cholesterol (1.11?0.19 vs 0.82?0.25 mmol/l; p = 0.005) and albumins (32.5?5.6 vs 29.5?3.7 g/l; p = 0.007) were statistically higher in the patients with metabolic syndrome than in patients without disturbance. Diabetes mellitus was a significant etiological factor of renal insufficiency in the patients with metabolic syndrome (p = 0.008). Conclusion. In our study approximately 30% of patients on haemodialysis had pronounced metabolic syndrome. The older, more obese men with increased levels of triglycireds and glucose in the serum dominated among them. Diabetes mellitus was a leading etiological factor of renal insufficiency in these patients.
Reliability of the CINtecTM p16INK4a immunocytochemical test in screening cervical precancerous lesions
Authors: Jovic Milena , Nenadic Dane , Magic Zvonko , Zoltarevski Lidija , Djurdjevic-Vukomanovic Biserka , Tatomirovic Zeljka , Strelic Natasa , Vojvodic Danilo
Published: December 2010

Background/Aim. Overexpression of p16INK4a has been found to be linked with genomic integration of high-risk human papillomavirus (HPV) and the developement of precancerous cervical intraepithelial lesions. The aim of this study was to examine is there a higher positive level of correlation between grade of histological dysplasia and p16INK4a level of expression in cervical smear, compared to results of Papanicolaou test. We also examined the correlation between HPV type, p16INK4a expression and Papanicolau test results. Methods. A total of 48 women with precanceorous cervical lesions and HPV cervicitis and 10 healthy women were enrolled in the study. Papanicolaou test, CINtecTM p16INK4a citological immunohistochemical test, polymerase chain reaction (PCR) HPV 16, 18, 31, 33 analysis and histopathology of the lesion were performed in all the patients. Results. Comparing the results of Papanicoulaou test and the grade of histological dysplasia, low-grade squamous intraepithelial lesion (LSIL) was confirmed in 38%, and high-grade squamous intraepithelial lesion (HSIL) in 69.2% of the patients (p > 0.05). Significant positive correlation was found between p16 overexpression and grade of histological dysplasia (p = 0.000). Overexpression p16 was found in 70% of LSIL and 94.4% of HSIL. Positive correlation was found between p16 overexpression and grade of dysplasia in Papanicolaou test (p = 0.011). In 38% of LSIL and 15% of HSIL cases p16 was not expressed. The most frequently found HPV type in PCR analysis was HPV16. Analysing the results of p16 test according to HPV status and Papanicolaou test rather heterogenous results were obtained. Conclusion. In the patients with precancerous cervical lesions a higher level of correlation was found between the grade of histological dysplasia and p16INK4a level of expression in the cervical smear, compared to the results of Papanicolaou test.
Application of monoclonal anti-CD20 antibody rituximab in the treatment of non-Hodgkin's lymphoma
Authors: Mihaljevic Biljana , Jankovic Snezana , Jakovic Ljubomir , Jovanovic-Perunicic Maja , Andjelic Bosko , Milosevic Violeta , Sretenovic Aleksandra , Virijevic Marjana , Petrovic Milan
Published: December 2010

 Nehockinski limfomi danas predstavljaju kamen temeljac savremenoj onkohematologiji, s obzirom na mogucnost visokog procenta njihovog izlecenja. Najcesca podgrupa limfoma su DBKL koji su kod oko 30% bolesnika izlecivi primenom konvencionalne terapije, dok su FL neizlecivi ukoliko se lece samo konvencionalnom terapijom. Istina je da se kod bolesnika sa FL terapijski odgovor postize brzo, ali recidivi se gotovo po pravilu desavaju i bolesnike je u svakom sledecem recidivu sve teze leciti. Sredinom devedestih godina napravljeno je prvo monoklonsko antitelo u lecenju malignih bolesti, i to najpre za recidive ili refraktarne FL. Ovo anti CD20 monoklonsko antitelo, rituksimab, predstavlja krucijalan napredak u lecenju limfoma, jer je primenom kombinoivane imunohemioterapije izlecenje DBKL poraslo na oko 60%, a poslednje studije ukazuju na mogucnost dugotrajnih remisija i PFS za FL, sto moze biti ekvivalent izlecenju i ove podgrupe limfoma. U toku su brojne studije kojima se vrse uporedne analize za primenu ritoksimaba i kod drugih podtipova limfoma kao sto je limfom marginalne zone, mantle celijski limfom, Hockinova bolest, a uvode se indikacije i za pojedine sistemske bolesti vezivnog tkiva, kao sto je reumatoidni artritis.
Concha bullosa and the nasal middle meatus obstructive syndrome
Authors: Peric Aleksandar , Sotirovic Jelena , Baletic Nenad , Kozomara Ruzica , Bijelic Dusan , Rasic Dejan
Published: December 2010

Background. Concha bullosa (CB) is pneumatization of the middle turbinate and one of the most common anatomic variation of the sinonasal region. It is found in about 25% of the population. Middle meatus obstructive syndrome (MMOS) is, usually connected with CB. The main symptoms of this syndrome are headaches, impaired nasal breathing and hyposmia. Headache is the most common symptom and it may occur due to contact between a CB and other structures of the nasal cavity. Case report. We presented a case of 32 year-old-woman with headaches, located in the orbital and the left frontal region. The headaches were intermittent and corresponding to the nasal cycle. After neurologic and alergic examination, endoscopic nasal examination demonstrated a septal deviation to the right side and a large middle turbinate in the left side of the nasal cavity. Coronal computerized tomography (CT) of the paranasal sinuses demonstrated the septal deformation and pneumatization of the left middle turbinate. Diagnosis was confirmed by lidocaine test. In the functional endoscopic surgery (FESS), the lateral lamela of the anterior CB was removed. At the same time, the septoplasty was done. At the control examination, the patient was without symptoms. Conclusion. Although CB is the common anatomic variation of the nasal cavity, MMOS is rare. Headache (rhinogenic origin) is the most important symptom. Surgical treatment is the lateral resection of the CB in the FESS technique and the septoplasty.
Plasmid profile analysis in identification of epidemic strains of Salmonella enterica serovar Enteritidis
Authors: Miljkovic-Selimovic Biljana , Lepsanovic Zorica , Babic Tatjana , Kocic Branislava , Randjelovic Gordana
Published: December 2010

Background/Aim. As illness caused by Sallmonella enterica serovar Enteritidis (S. Enteritidis) occurs not only as sporadic cases but as outbreaks, to reveal the source and routes of spreading of infection it is necessary to identify epidemic strain by the use of some typing methods. To determine whether plasmid profile analysis, as genotyping method, could be applied for the investigation of epidemic strains, isolates of S. Enteritidis, recovered from patient's stools and food associated with outbreaks and those isolated from sporadic cases of diarrhea, were investigated. Methods. Investigation of antibiotic resistance was performed by Kirby - Bauer disc-diffusion method. Isolation of plasmid DNA was carried out by Birnboim and Dolly alkaline lysis method, modified by Ish-Horovitz. Results. Out of 276 izolates of S. Enteritidis 94 were isolated from patient's stools and food associated with outbreaks and 182 were isolated from sporadic cases of diarrhea. The presence of 12 plasmid profiles was established. An average correlation degree of plasmid profiles between the strains was 0.84, that implies high degree of similarity of plasmid profiles of epidemic and non- epidemic strains isolated at our geographic region for the given period of time. Conclusion. The strains of S. Enteritidis, isolated in outbreaks of enterocolitis as well as from spordic cases of diarrhea in the same period of time and at the same area, frequently exhibit the same plasmid profile characterized by a single plasmid of 38 MDa. Therefore, in most cases plasmid profile analysis is not valuable in the identification of epidemic strains of S. Enteritidis. However, for this purpose plasmid profile analysis could be used when drug-resistant strains of S. Enteritidis are isolated, as they often possess additional resistant plasmids what increases discrimination power of this method.
Argon-plasma coagulation as a primary therapeutic method for the treatment of flat duodenal adenomas
Authors: Knezevic Slavko , Ugljesic Milenko , Micev Marjan , Krstic Miodrag , Stojkovic Mirjana
Published: December 2010

Background. Argon-plasma coagulation is a method for tissue coagulation that uses high-frequency electric energy and ionized argon gas. It is used in endoscopic haemostasis and in coagulation of smaller, superficial lesion on gastrointestinal mucosa such as flat adenomas, but also in reduction of tumor tissue. Case report. We presented a patient with flat adenoma duodeni. Adenoma had been treated with argon plasma coagulation, in one act, and with a complete restitution of mucosa in further follows up. Conclusion. Argon plasma can be efficiently used in coagulation of superficial lesion of gastrointestinal mucosa, that belongs to the type of flat adenomas, as well as other superficial lesions of mucosa that require endoscopic mucosectomy from smaller, bordered spaces.
External fixation in the treatment of open tibial shaft fractures
Authors: Golubovic Zoran , Stojiljkovic Predrag , Macukanovic-Golubovic Lana , Milic Dragan , Milenkovic Sasa , Kadija Marko , Matovic Zoran , Turkovic Goran , Radenkovic Mile , Visnjic Aleksandar , Golubovic Ivan , Stojanovic Sasa , Vidic Goran , Mitkovic Milorad
Published: December 2010

Background/Aim. Besides the conquasant fractures, open tibia shaft fractures belong to the group of the most severe fractures of tibia. Open tibia shaft fracture is one of the most common open fractures of long bones. They most frequently occur as a result of traffic accidents caused by the influence of a strong direct force. Methods. Within the period from January, 2000 to December 31, 2005. at the Clinic for Orthopaedics and Traumatology, Clinical Center Nis, 107 patients with open tibial fractures were treated. We analyzed 96 patients with open tibial fracture. In the series analyzed, the male sex was prevalent - there were 74 men (77.08%). The mean age was 47.3 years. The youngest patient was 17 years old, while the oldest patient was 79. According to the classification of the Gustilo et al. in the analysed group there were 30 (31.25%) open tibial fractures of the I degree, 31 (32.29%) of the II degree, 25 (26.05%) of the III A degree, 8 (8.33%) of the III B degree and 2 (2.08%) of the III C degree. In 95 of the patients the treatment of open tibia shaft fractures consisted of the surgical treatment of wound and the external fixation of the fractured bone using "Mitkovic" type external fixator with a convergent method of pin applications. One primary amputations had been done in patients with grade IIIC open tibial fracture with large soft tissue defect. Results. Of the 96 open tibial fractures available for follow-up, 73 (76.04%) healed without severe complications (osteitis, pseudoarthrosis, valgus malunion and amputation). Ther were nine (9.38%) soft tissue pin track infections and six (6.25%) superficial wound infections. The mean time of union was 21 (14-36) week. Among severe local complications associated with open tibial fractures, in eight patients (8.33%) was registered osteitis, and in nine patients (9.38%) fracture nonunion and the development of pseudoarthrosis. Three of the patients (3.13%) had more than 10 degree valgus malunion. In one patients (1.04%) deep pin track infection developed. Two patients (2.08%) had below the knee amputation (one primary in patient with type III C open fracture and one secondary after the development of deep infections). Conclusion. Surgical treatment of wounds, external fixation, leaving the wounds open and performing necessary debridements, adequate drug therapy administration are essential for obtaining good results in patients with open tibial shaft fractures.
Early results of liver resection due to metastases of colorectal carcinoma
Authors: Koprivica Radenko , Radevic Bozina , Tosovic Goran , Koprivica Ranka , Smiljanic Radmila
Published: December 2010

Background/Aim. Liver metastases are most frequently the result of colorectal carcinoma. The aim of this study was to analyse early results of operative treatment of the patients with the liver metastases of colorectal carcinoma. Methods. This retrospective, prospective study included 387 patients with colorectal carcinoma operated during the period from 2005-2007. All the patients were submitted to oncologic surgery protocol. The functional state of the liver was assessed, especially in the patients with hemotherapy. Diagnostic protocol further included color Doppler ultrasonography of the liver and port system, as well as spiral computed tomography includy angiography. Nine (5.7%) of the patients were submitted to explorative laparotomy. Results. Of all the patients 157 (40.6%) had metastases in the liver, synhronous 78 (20.15%) and metachronous 79 (20.45%), Forty two (26.7%) patients were indicated for the liver resection. In 33 (21%) of them it was performed successfully. There were 18 females and 15 males of the average age 60.09 (42-81) years. Up to 4 metastases had 90.9% of the patients and in 9.09% had up to 5 and more metastases. On average, metastases occupied 2.6 of the liver segments. There were 21.2% of solitary lesions (7 patients), 63.6% of multilple unilobular (21 patients) and 15.15% of multiple bilobular metastases (5 patients). Liver resection was done using ultraharmonic scalpel. At spacious liver resections we used a device for intraoperative saving of blood (cell saver) and applied a technique of selective hepatic vascular exclusion. An average disease-free interval was 16.7 months. There were 60.6% of anatomical liver resections and 39.4% of atypical resections. In 31.1% of the operated patients we made resection on up to two segments of the liver and in 30.3% we made resection of four segments. An average number of segments where resection had been performed was 2.65 and the duration of operation was 143 minutes. In 39.4% of the cases we used a technique of liver vascular isolation, an average duration was 38 minutes. In 30.3% of resections we used cell saver and 70% of operated patients were transfused. On average, 493 ml of blood was transfused. Of the total number of resections, 90.9% was of the type R0, 9.09% of the type R1. An average duration of postoperative hospitalization was 10.6 days. Operative morbidity rate was 15.15% and operative mortality 3.03%. During a six-month observing there were not any mortality or repeated metastases in liver. Conclusion. Anatomic liver resection including selective vascular hepatic excision by the use of an ultraharmonic scalpel and cell saver is considered to be efficient and secure method for the reduction of intraoperative and postoperative complications rendering good surgical results.
Effect of recasting on the thickness of metal-ceramic interface of nickel-chromium and cobalt-chromium alloys
Authors: Mirkovic Nemanja , Draganjac Miroslav , Stamenkovic Dragoslav , Ristic Ljubisa
Published: December 2010

Introduction/Aim. This research was done to establish recasting effects of nickel-chromium and cobalt-chromium alloys on the thickness of their metal-ceramic interface in making fixed partial dentures. Metal-ceramic interface determines their functional integrity and prevents damages on ceramics during mastication. Investigation of metal-ceramic samples is supposed to show if base metal alloys for metalceramics are successfully recycled without any risk of reduction of metal-ceramic interface thickness. Methods. The research was performed as an experimental study. Per six metal-ceramic samples of nickel-chromium alloy (Wiron99) and cobalt-chromium alloy (Wirobond C) were made each. Alloy residues were recycled through twelve casting generations with the addition of 50% of new alloy on the occasion of every recasting. Analysis Energy Dispersive X-ray (EDX) (Oxford Instruments) and Scanning Electon Microscop (SEM) analysis (JEOL) were used to determine thickness of metal-ceramic interface together with PC Software for quantification of visual information's (KVI POPOVAC). Results. Results of this research introduced significant differences between thickness of metal-ceramic interface in every examined recycle generation. Recasting had negative effect on thickness of metal-ceramic interface of the examined alloys. This research showed almost linear reduction of elastic modulus up to the 12th generation of recycling. Conclusion. Recasting of nickel-chromium and cobaltchromium alloys is not recommended because of reduced thickness of metal-ceramic interface of these alloys. Instead of recycling, the alloy residues should be returned to the manufacturers.
Age as a risk factor for suicide
Authors: Kocic Sanja , Milic Caslav , Grbic Gordana , Plasic Aleksandar , Tatic Zoran
Published: December 2010

Background/Aim. World Health Organization (WHO) in its plan for health policy until the year 2010, has taken reduction of risk factors of suicide as its 12th aim. Because of the fact that the problem of suicide is also significant health problem in our society, the aim of this study was to examine the influence of life period as a risk factor for suicide in the area of the town of Kragujevac. Methods. In total 211 persons, both sexes, aged between 17 and 91 years, from the area of the town of Kragujevac, who had been committed a suicide during the period from 1996 to 2005, were included in a retrospective study. This study included the analysis of: conditions prior to suicide, locations of suicide, motives for suicide, the ways of committing suicide. For statistical analysis ?2 test and univariante regression model were used. Results. Average rate of suicide, in analyzed period, moved from 8.7 to 27 with a mean value of 14.6? 6.9. Suicide rates were the lowest in the age group from 15 to 24 years and the highest in the age group above 65 years (p < 0.05). Among the presuicidal conditions, within any age groups the presence of mental disease dominated as a factor for suicide, but within the oldest one in which organic diseases prevailed as a factor for suicide (p < 0.05). Statistically significant fact is that a house (flat) was the main location for committing suicide in any age groups. Motives for suicide were significantly different within the groups and they were mostly unknown. Committing suicide by hanging was the most frequent way of suicide among any age groups. Univariant regression analysis failed to show any impact of age on the analyzed factors. Conclusion. Because of the fact that an average rate of suicide in elderly increases it is obligatory to primarily determine risk factors for suicide among people more than 65 years of age. Physicians should play the most important role in that.
Hypocretin neurotransmitter system and narcolepsy
Authors: Lakocevic Milan , Rajsic Nenad
Published: December 2010

 Prelomno otkrice nedostatka hipokretina kod narkolepsije pruza izvrsnu osnovu za bolje razumevanje fiziologije spavanja i podsticaj je za buduca istrazivanja. Medjutim, ono nije omogucilo da se razresi enigma etiologije narkolepsije. Za razliku od animalnih modela, narkolepsija kod ljudi, mada specificno povezana sa deficitom hipokretina, nije samo jednostavan genski poremecaj. Mehanizam regulisanja ekspresije gena za preprohipokretin tek treba da bude razjasnjen.
Analysis of dimensions of sella turcica in patients with mandibular prognathism
Authors: Cutovic Tatjana , Pavlovic Jasna , Kozomara Ruzica
Published: December 2010

Introduction/Aim. Patients with mandibular prognathism as dominant symptom have disordered sagittal interjaw relations that make prominent appearance to this dental craniofacial anomaly beside hyperplastic mandibles and inverted front teeth overlap. The aim of this study was to examine the differences in dimensions of sella turcica in patients with mandibular prognathism and in eugnathic. Methods. On profile teleradiographs of 30 eugnathic control and 30 patients with mandibular prognathism, three parameters, which represent dimensions of sella turcica, were measured (surface, width and depth). Results. Statistically significant difference in values between the groups was found. All the three measured parameters were significantly higher in the patients with mandibular prognathism (p < 0.01). Conclusion. In the patients with mandibular prognathism all the measured dimensions of sella turcica were bigger, and so was sella turcica, but that enlargement was not in correlation with the degree of anomaly itself.