bluuebunny/crossref_metadata_2025 huggingface dataset:
Insights Into the Mn(VII) and Cr(VI) Adsorption Mechanisms on Purified Diatomite/MCM-41 Composite: Experimental Study and Statistical Physics Analysis
Authors: Ahmed Inas A. , Badawi Michael , Bonilla-Petriciolet Adrián , Lima Eder C. , Seliem Moaaz K. , Mobarak Mohamed
Published: February 2022

In this study, a purified diatomite (PD) with a concentration of diatom frustules more than 92% SiO2 was utilized to synthesize a composite of MCM-41 silica under hydrothermal conditions. The as-synthesized PD/MCM-41 composite was characterized and tested as an adsorbent for the removal of Cr(VI) and Mn(VII) ions from aqueous solution. Results of X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and Fourier-transform infrared spectroscopy (FTIR) revealed that the diatom frustules of the PD were coated with MCM-41 mesoporous silica. Experimental isotherms of Cr(VI) and Mn(VII) adsorption were fitted to classical and advanced statistical physics models at 25°C–55°C and pH 3. The Langmuir model estimated monolayer adsorption capacities ranging from 144.1 to 162.2 mg/g for Cr(VI) and 166.2 to 177.0 mg/g for Mn(VII), which improved with increasing the solution temperature. Steric and energetic parameters obtained from a monolayer adsorption model with one adsorption site was utilized to explain the adsorption mechanism at a microscopic level. The number of Cr(VI) and Mn(VII) ions adsorbed on PD/MCM-41 active site (n) were 1.25–1.27 for Cr(VI) and 1.27–1.32 for Mn(VII), thus suggesting multi-interaction mechanisms. The density of PD/MCM-41 active sites (DM) was a key parameter to explain the adsorption of these heavy metals. The adsorbed quantities were maximum at 55°C, thus obtaining 102.8 and 110.7 mg/g for Cr(VI) and Mn(VII), respectively. Cr(VI) and Mn(VII) adsorption energies ranged from 18.48 to 26.70 kJ/mol and corresponded to an endothermic adsorption with physical forces. Entropy, free enthalpy, and internal energy associated to the adsorption of Cr(VI) and Mn(VII) ions were calculated, thus indicating that the removal of these pollutants was spontaneous. Overall, this article offers new interpretations for the Cr(VI) and Mn(VII) adsorption mechanisms on PD/MCM-41 composite, which are relevant to contribute to the development of effective water treatment processes.
Pyroligneous Acids of Differently Pretreated Hybrid Aspen Biomass: Herbicide and Fungicide Performance
Authors: Korkalo Pasi , Hagner Marleena , Jänis Janne , Mäkinen Marko , Kaseva Janne , Lassi Ulla , Rasa Kimmo , Jyske Tuula
Published: February 2022

The pyroligneous acids (PAs) of woody biomass produced by torrefaction have pesticidal properties. Thus, PAs are potential alternatives to synthetic plant protection chemicals. Although woody biomass is a renewable feedstock, its use must be efficient. The efficiency of biomass utilization can be improved by applying a cascading use principle. This study is novel because we evaluate for the first time the pesticidal potential of PAs derived from the bark of hybrid aspen (Populus tremula L. × Populus tremuloides Michx.) and examine simultaneously how the production of the PAs can be interlinked with the cascade processing of hybrid aspen biomass. Hybrid aspen bark contains valuable extractives that can be separated before the hemicellulose is thermochemically converted into plant protection chemicals. We developed a cascade processing scheme, where these extractives were first extracted from the bark with hot water (HWE) or with hot water and alkaline alcohol (HWE+AAE) prior to their conversion into PAs by torrefaction. The herbicidal performance of PAs was tested using Brassica rapa as the test species, and the fungicidal performance was proven using Fusarium culmorum. The pesticidal activities were compared to those of the PAs of debarked wood and of commercial pesticides. According to the results, extractives can be separated from the bark without overtly diminishing the weed and fungal growth inhibitor performance of the produced PAs. The HWE of the bark before its conversion into PAs appeared to have an enhancing effect on the herbicidal activity. In contrast, HWE+AAE lowered the growth inhibition performance of PAs against both the weeds and fungi. This study shows that hybrid aspen is a viable feedstock for the production of herbicidal and fungicidal active chemicals, and it is possible to utilize biomass according to the cascading use principle.
Molecular Approaches to Protein Dimerization: Opportunities for Supramolecular Chemistry
Authors: Dang Dung Thanh
Published: February 2022

Protein dimerization plays a key role in many biological processes. Most cellular events such as enzyme activation, transcriptional cofactor recruitment, signal transduction, and even pathogenic pathways are significantly regulated via protein-protein interactions. Understanding and controlling the molecular mechanisms that regulate protein dimerization is crucial for biomedical applications. The limitations of engineered protein dimerization provide an opportunity for molecular chemistry to induce dimerization of protein in biological events. In this review, molecular control over dimerization of protein and activation in this respect are discussed. The well known molecule glue-based approaches to induced protein dimerization provide powerful tools to modulate the functionality of dimerized proteins and are shortly highlighted. Subsequently metal ion, nucleic acid and host-guest chemistry are brought forward as novel approaches for orthogonal control over dimerization of protein. The specific focus of the review will be on host-guest systems as novel, robust and versatile supramolecular approaches to modulate the dimerization of proteins, using functional proteins as model systems.
Lignin-Based/Polypyrrole Carbon Nanofiber Electrode With Enhanced Electrochemical Properties by Electrospun Method
Authors: Hu Zhou-Rui , Li Dan-Dan , Kim Tae-Hee , Kim Min-Seok , Xu Ting , Ma Ming-Guo , Choi Sun-Eun , Si Chuanling
Published: February 2022

Tailoring the structure and properties of lignin is an important step toward electrochemical applications. In this study, lignin/polypyrrole (PPy) composite electrode films with microporous and mesoporous structures were designed effectively by electrostatic spinning, carbonization, and in situ polymerization methods. The lignin can not only reduce the cost of carbon fiber but also increase the specific surface area of composite films due to the removal of carbonyl and phenolic functional groups of lignin during carbonization. Besides, the compact three-dimensional (3D) conductive network structures were constructed with PPy particles densely coated on the lignin nanofibers, which was helpful to improve the conductivity and fast electron transfer during the charging and discharging processes. The synthesized lignin carbon fibers/PPy anode materials had good electrochemical performance in 1 M H2SO4 electrolyte. The results showed that, at a current density of 1 A g−1, the lignin carbon nanofibers/PPy (LCNFs/PPy) had a larger specific capacitance of 213.7 F g−1 than carbon nanofibers (CNFs), lignin carbon nanofibers (LCNFs), and lignin/PPy fiber (LPAN/PPy). In addition, the specific surface area of LCNFs/PPy reached 872.60 m2 g−1 and the average pore size decreased to 2.50 nm after being coated by PPy. Therefore, the independent non-binder and self-supporting conductive film is expected to be a promising electrode material for supercapacitors with high performance.
Predicting Pulmonary Function From the Analysis of Voice: A Machine Learning Approach
Authors: Alam Md. Zahangir , Simonetti Albino , Brillantino Raffaele , Tayler Nick , Grainge Chris , Siribaddana Pandula , Nouraei S. A. Reza , Batchelor James , Rahman M. Sohel , Mancuzo Eliane V. , Holloway John W. , Holloway Judith A. , Rezwan Faisal I.
Published: February 2022

Introduction

To self-monitor asthma symptoms, existing methods (e.g. peak flow metre, smart spirometer) require special equipment and are not always used by the patients. Voice recording has the potential to generate surrogate measures of lung function and this study aims to apply machine learning approaches to predict lung function and severity of abnormal lung function from recorded voice for asthma patients.

Methods

A threshold-based mechanism was designed to separate speech and breathing from 323 recordings. Features extracted from these were combined with biological factors to predict lung function. Three predictive models were developed using Random Forest (RF), Support Vector Machine (SVM), and linear regression algorithms: (a) regression models to predict lung function, (b) multi-class classification models to predict severity of lung function abnormality, and (c) binary classification models to predict lung function abnormality. Training and test samples were separated (70%:30%, using balanced portioning), features were normalised, 10-fold cross-validation was used and model performances were evaluated on the test samples.

Results

The RF-based regression model performed better with the lowest root mean square error of 10·86. To predict severity of lung function impairment, the SVM-based model performed best in multi-class classification (accuracy = 73.20%), whereas the RF-based model performed best in binary classification models for predicting abnormal lung function (accuracy = 85%).

Conclusion

Our machine learning approaches can predict lung function, from recorded voice files, better than published approaches. This technique could be used to develop future telehealth solutions including smartphone-based applications which have potential to aid decision making and self-monitoring in asthma.
Exploring Methodological Approaches for Strengthening the Resilience of Coastal Flood Protection System
Authors: Igigabel Marc , Diab Youssef , Yates Marissa
Published: February 2022

Coastlines are subject to multiple developments related to land use planning and the effects of climate change. These developments generally lead to an increase in the risk of coastal flooding. This article focuses on systems to protect against this hazard, and presents a bibliographical analysis on methods and operational tools to strengthen their resilience. This analysis is carried out by considering that a system of protection is a component of the territory to which it provides protection and that it is therefore necessary to study in depth the relations between this system and the various components of the territory (the natural environment, the built environment and the social and institutional environment). Based on this hypothesis, the concepts of risk and resilience applied to floods are specified and the protection and adaptation strategies commonly used in recent decades are described. This retrospective on concepts and strategies leads us to structure the state-of-the-art analysis on methods and operational tools in relation to two issues: 1) understanding risks; 2) adapting and transforming protection systems. In each case, the use of the concept of resilience implies a clear distinction between systemic and analytical approaches. Finally, this bibliographical analysis reveals the need to revise the protection system concept in order to promote the integration of these systems in the territories. Accordingly, new methodological developments could be considered using ecosystem and socio-economic approaches.
Detecting Interdecadal Change in Western North Pacific Tropical Cyclone Genesis Based on Cluster Analysis Using pHash + Kmeans
Authors: Tian Ye , Zhou Wen , Wong W. K.
Published: February 2022

Previous studies have noted an abrupt decrease in western North Pacific (WNP) tropical cyclone (TC) genesis frequency and a westward shift in genesis location since the late 1990s. The recent application of cluster analysis in TC research shows the effect of detecting the contribution of the Western North Pacific Subtropical High (WNPSH) and the interdecadal Pacific oscillation (IPO) on interdecadal change in WNP TCs. In this work, we also apply a clustering algorithm called pHash + Kmeans to group WNP TCs into three classes based on their genesis environmental conditions. The clustering results show that an abrupt decrease after 1998 is related primarily to a decrease in the dominant class (Class3, located mainly in the southern and eastern WNP), and an increase after 2010 occurs because of a new dominant class (Class1, located mainly in the northwestern WNP), which indicates that the WNP environment suppresses Class3 genesis after 1998 and enhances Class1 genesis after 2010. Three periods (P1: 1979–1997, P2: 1998–2010, and P3: 2011–2020) and three regions (SCS: 100°E-120°E, EQ-30°N; WNP1: 120°E-140°E, EQ-30°N; and WNP2: 140°E-160°W, EQ-30°N) are divided to further confirm the above findings. In P1, high (low) mid-level relative humidity (RH), intense (weak) low-level vorticity, and weak (strong) vertical wind shear (VWS) are distributed in WNP2 (SCS and WNP1), indicating suitable environmental conditions for TC genesis in WNP2 but unsuitable conditions in SCS and WNP1. This situation is the opposite in P2, leading to a decrease in genesis frequency and a westward shift in genesis location. In P3, strong low-pressure vorticity and thermodynamic conditions occur in SCS and WNP1, contributing to an increase in TC genesis frequency.
Frequency of Pyrethroid Insecticide Resistance kdr Gene and Its Associated Enzyme Modulation in Housefly, Musca domestica L. Populations From Jhang, Pakistan
Authors: Riaz Bushra , Kashif Zahoor Muhammad , Malik Kausar , Ahmad Aftab , Majeed Humara Naz , Jabeen Farhat , Zulhussnain Muhammad , Ranian Kanwal
Published: February 2022

Housefly, Musca domestica, is considered responsible for transmitting a wide variety of human and veterinary diseases. Mostly, insecticides are being used for their control and more commonly, pyrethroid insecticides worldwide. However, resistance has been reported against various pyrethroid insecticides. Houseflies become resistant by two major mechanisms, i.e., target site insensitivity through knockdown resistance gene mutation (kdr) and enzyme detoxification. Thus, the current study was designed to monitor the frequency of pyrethroid resistance gene kdr in housefly populations of District Jhang. The flies were collected from seven sampling sites and then reared in the lab for molecular and biochemical assays. The amplification of template DNA was performed for knockdown resistance gene through the outer primers kdr1 and kdr4, and the inner primers kdr1 and kdr2 using PASA (PCR Amplification of Specific Alleles) method which specifically amplify the domain-II of kdr gene. Three populations were found homozygous susceptible (+/+; 42.85%), whereas two populations were found genetically homozygous resistant (−/−; 28.57%) which are insensitive to pyrethroid insecticides. Similarly, two populations were found heterozygous (+/−; 28.57%) for kdr suggesting thereby that at least 1/4th homozygous-resistant (−/−) housefly populations with insensitivity to pyrethroids would be produced in the future keeping in view the Mendelian ratio. Biochemical assay showed that homozygous-resistant populations had increased activity of Acetylcholinesterase (AChE), α-Carboxylesterases (α-Carboxyl), β-Carboxylesterase (β-Carboxyl), Alkaline Phosphatase (AkP), and Acidic Phosphatase (AcP) enzymes. In addition, heterozygous populations also showed increased activities of these enzymes. The current results would not only help avoid the indiscriminate load of insecticides onto the environment but also serve as a hallmark for the management of housefly populations in target areas in the future.
Identification and Validation of Aging-Related Genes in Idiopathic Pulmonary Fibrosis
Authors: He Jie , Li Xiaoyan
Published: February 2022

Aging plays a significant role in the occurrence and development of idiopathic pulmonary fibrosis (IPF). In this study, we aimed to identify and verify potential aging-associated genes involved in IPF using bioinformatic analysis. The mRNA expression profile dataset GSE150910 available in the Gene Expression Omnibus (GEO) database and R software were used to identify the differentially expressed aging-related genes involved in IPF. Hub gene expression was validated by other GEO datasets. Gene ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were performed on differentially expressed aging-related genes. Subsequently, aging-related genes were further screened using three techniques (least absolute shrinkage and selection operator (LASSO) regression, support vector machine, and random forest), and the receiver operating characteristic curves were plotted based on screening results. Finally, real-time quantitative polymerase chain reaction (qRT-PCR) was performed to verify the RNA expression of the six differentially expressed aging-related genes using the blood samples of patients with IPF and healthy individuals. Sixteen differentially expressed aging-related genes were detected, of which the expression of 12 were upregulated and four were downregulated. GO and KEGG enrichment analyses indicated the presence of several enriched terms related to senescence and apoptotic mitochondrial changes. Further screening by LASSO regression, support vector machine, and random forest identified six genes (IGF1, RET, IGFBP2, CDKN2A, JUN, and TFAP2A) that could serve as potential diagnostic biomarkers for IPF. Furthermore, qRT-PCR analysis indicated that among the above-mentioned six aging-related genes, only the expression levels of IGF1, RET, and IGFBP2 in patients with IPF and healthy individuals were consistent with the results of bioinformatic analysis. In conclusion, bioinformatics analysis identified 16 potential aging-related genes associated with IPF, and clinical sample validation suggested that among these, IGF1, RET, and IGFBP2 might play a role in the incidence and prognosis of IPF. Our findings may help understand the pathogenesis of IPF.
Sustainability of the Effects and Impacts of Using Digital Technology to Extend Maternal Health Services to Rural and Hard-to-Reach Populations: Experience From Southwest Nigeria
Authors: Akeju David , Okusanya Babasola , Okunade Kehinde , Ajepe Adegbenga , Allsop Matthew J. , Ebenso Bassey
Published: February 2022

Background

Nigeria has one of the worst health and development profiles globally. A weak health system, poor infrastructure, and varied socio-cultural factors are cited as inhibitors to optimal health system performance and improved maternal and child health status. eHealth has become a major solution to closing these gaps in health care delivery in low- and middle-income countries (LMICs). This research reports the use of satellite communication (SatCom) technology and the existing 3G mobile network for providing video training (VTR) for health workers and improving the digitization of healthcare data.

Objective

To evaluate whether the expected project outcomes that were achieved at the end-line evaluation of 2019 were sustained 12 months after the project ended.

Methods

From March 2017 to March 2019, digital innovations including VTR and data digitization interventions were delivered in 62 healthcare facilities in Ondo State, southwest Nigeria, most of which lacked access to a 3G mobile network. Data collection for the evaluation combined documents' review with quantitative data extracted from health facility registers, and 24 of the most significant change stories to assess the longevity of the outcomes and impacts of digital innovation in the four domains of healthcare: use of eHealth technology for data management, utilization of health facilities by patients, the standard of care, and staff attitude. Stories of the most significant changes were audio-recorded, transcribed for analysis, and categorized by the above domains to identify the most significant changes 12 months after the project closedown.

Results

Findings showed that four project outcomes which were achieved at end-line evaluation were sustained 12 months after project closedown namely: staff motivation and satisfaction; increased staff confidence to perform healthcare roles; improved standard of healthcare delivery; and increased adoption of eHealth innovations beyond the health sector. Conversely, an outcome that was reversed following the discontinuation of SatCom from health facilities is the availability of accurate and reliable data for decision-making.

Conclusion

Digital technology can have lasting impacts on health workers, patients, and the health system, through improving data management for decision-making, the standard of maternity service delivery, boosting attendance at health facilities, and utilization of services. Locally driven investment is essential for ensuring the long-term survival of eHealth projects to achieve sustainable development goals (SDGs) in LMICs.
The Role of Innate Immunity in Natural Elite Controllers of HIV-1 Infection
Authors: Shi Yuting , Su Jinming , Chen Rongfeng , Wei Wudi , Yuan Zongxiang , Chen Xiu , Wang Xinwei , Liang Hao , Ye Li , Jiang Junjun
Published: February 2022

The natural process of human immunodeficiency virus type 1(HIV-1) infection is characterized by high viral load, immune cell exhaustion, and immunodeficiency, which eventually leads to the stage of acquired immunodeficiency syndrome (AIDS) and opportunistic infections. Rapidly progressing HIV-1 individuals often die of AIDS several years after infection without treatment. The promotion of ART greatly prolongs the survival time of HIV-infected persons. However, some patients have incomplete immune function reconstruction after ART due to latent storage of HIV-infected cells. Therefore, how to achieve a functional cure has always been the focus and hot spot of global AIDS research. Fortunately, the emergence of ECs/LTNPs who can control virus replication naturally has ignited new hope for realizing a functional cure for AIDS. Recently, a special category of infected individuals has attracted attention that can delay the progression of the disease more rigorously than the natural progression of HIV-1 infection described above. These patients are characterized by years of HIV-1 infection, long-term asymptomatic status, and normal CD4+T cell count without ART, classified as HIV-infected long-term nonprogressors (LTNPs) and elite controllers (ECs). Numerous studies have shown that the host and virus jointly determine the progression of HIV-1 infection, in which the level of innate immunity activation plays an important role. As the first line of defense against pathogen invasion, innate immunity is also a bridge to induce adaptive immunity. Compared with natural progressors, innate immunity plays an antiviral role in HIV-1 infection by inducing or activating many innate immune-related factors in the natural ECs. Learning the regulation of ECs immunity, especially the innate immunity in different characteristics, and thus studying the mechanism of the control of disease progression naturally, will contribute to the realization of the functional cure of AIDS. Therefore, this review will explore the relationship between innate immunity and disease progression in ECs of HIV-1 infection from the aspects of innate immune cells, signaling pathways, cytokines, which is helpful to provide new targets and theoretical references for the functional cure, prevention and control of AIDS, and development of a vaccine.
Cadherin-11 Regulates Macrophage Development and Function
Authors: To Sarah , Chavula Thandiwe , Pedroza Mesias , Smith Jennifer , Agarwal Sandeep K.
Published: February 2022

Cadherin-11 (CDH11) is a cell-cell adhesion protein that has previously been reported to play an important role in the pathogenesis of pulmonary fibrosis. It is expressed on macrophages in the fibrotic lung. However, the role of CDH11 on macrophage biology has not yet been studied. We show using immunophenotypic analyses thatCdh11-/-mice have fewer recruited monocyte-derived macrophages and Ly6Chimonocytes in the lungs compared to wild-type mice in the intraperitoneal bleomycin-induced pulmonary fibrosis model. Additionally, fewer Ly6Chimonocytes were detected in the bone marrow and peripheral blood of naiveCdh11-/-mice. Given that macrophages are derived from monocytes, we investigated the precursors of the monocyte/macrophage lineage in the bone marrow. We found increased numbers of CMPs and reduced numbers of GMPs and MPs/cMoPs inCdh11-/-mice compared to wild-type mice, suggesting decreased differentiation towards the myeloid lineage inCdh11-/-mice. Furthermore, we show using bone marrow cells that loss of CDH11 impaired monocyte to macrophage differentiation. We also demonstrate that CDH11 deficiency repressed the M2 program and impaired the phagocytic function of bone marrow-derived macrophages. Overall, our findings demonstrate a role for CDH11 in macrophage development, M2 polarization, and phagocytic function.
Long-Term Analysis of Pertussis Vaccine Immunity to Identify Potential Markers of Vaccine-Induced Memory Associated With Whole Cell But Not Acellular Pertussis Immunization in Mice
Authors: Weaver Kelly L. , Blackwood Catherine B. , Horspool Alexander M. , Pyles Gage M. , Sen-Kilic Emel , Grayson Emily M. , Huckaby Annalisa B. , Witt William T. , DeJong Megan A. , Wolf M. Allison , Damron F. Heath , Barbier Mariette
Published: February 2022

Over two decades ago acellular pertussis vaccines (aP) replaced whole cell pertussis vaccines (wP) in several countries. Since then, a resurgence in pertussis has been observed, which is hypothesized to be linked, in part, to waning immunity. To better understand why waning immunity occurs, we developed a long-term outbred CD1 mouse model to conduct the longest murine pertussis vaccine studies to date, spanning out to 532 days post primary immunization. Vaccine-induced memory results from follicular responses and germinal center formation; therefore, cell populations and cytokines involved with memory were measured alongside protection from challenge. Both aP and wP immunization elicit protection from intranasal challenge by decreasing bacterial burden in both the upper and lower airways, and by generation of pertussis specific antibody responses in mice. Responses to wP vaccination were characterized by a significant increase in T follicular helper cells in the draining lymph nodes and CXCL13 levels in sera compared to aP mice. In addition, a population of B. pertussis+ memory B cells was found to be unique to wP vaccinated mice. This population peaked post-boost, and was measurable out to day 365 post-vaccination. Anti-B. pertussis and anti-pertussis toxoid antibody secreting cells increased one day after boost and remained high at day 532. The data suggest that follicular responses, and in particular CXCL13 levels in sera, could be monitored in pre-clinical and clinical studies for the development of the next-generation pertussis vaccines.
Dynamics of Probable Maximum Precipitation Within Coastal Urban Areas in a Convection-Permitting Regional Climate Model
Authors: Liang Ju , Yong Yangyang
Published: February 2022

The west coast of Canada is strongly affected by the extreme precipitation events triggered by frequent atmospheric river (AR) activities over the eastern North Pacific. Across the region, assessing the probable maximum precipitation (PMP), can provide valuable information for resilience building of the coastal communities that are vulnerable to hydrological risks. In this study, a 3-km convection-permitting regional climate model is used to physically estimate the PMP in Vancouver. This technique maximizes the effect of AR-related water vapor transport by spatially adjusting the lateral boundary conditions (LBCs) of the model simulations for the selected AR-related extreme precipitation events. The PMP in Vancouver is identified among the simulations driven by the spatially adjusted LBCs that are corresponding with the AR-induced “worst-case scenario,” i.e., landfalling ARs hit Vancouver with optimal landfalling location and transport direction. Results suggest that the PMP in Vancouver, in terms of the maxima of the regionally averaged 72-h total precipitation for the historical extreme precipitation events, is up to 790 mm, which is 130% greater than the historical peak precipitation for the period 1980∼2017. On average, all the PMP simulations shows an overall increase by 81% in precipitation by relative to historical simulations. In addition, the PMP simulations suggested an overall decrease in snowfall by 12% due to the warmer near-surface air temperature; however, a pronounced increase in freezing rain is seen. The precipitation increase for the estimated PMP relative to the historical extreme precipitation is closely associated with the increased atmospheric moisture transport and the changes in the atmospheric dynamic factors when the AR effects are maximized. These include the enhanced low-tropospheric ascent and moisture transport convergence, which can induce stronger depletion of atmospheric moistures as indicated by the increased precipitation efficiency.
The Morphology, Mitogenome, Phylogenetic Position, and Symbiotic Bacteria of a New Species of Sclerolinum (Annelida: Siboglinidae) in the South China Sea
Authors: Xu Ting , Sun Yanan , Wang Zhi , Sen Arunima , Qian Pei-Yuan , Qiu Jian-Wen
Published: February 2022

Sclerolinum annulatusn. sp. (Annelida: Siboglinidae) is described based on specimens collected from soft sediment of the Haima cold seep in the South China Sea. Morphologically,S.annulatusn. sp. is distinct in having a tube with transverse rings and a forepart (i.e., anterior region) containing one arched row of elongated plaques on both sides of the dorsal furrow. Genome skimming, assembly, and annotation produced a nearly complete mitogenome ofS. annulatusn. sp. with 15,553 bp nucleotides that encodes 13 protein-coding genes, two rRNA, and 22 tRNA. Phylogenetic analyses based on the mitochondrialcytochrome c oxidase I(cox1) gene and a concatenated dataset comprising the mitochondrialcox1and16S rRNAgenes along with the nuclear18S rRNAgene both strongly support the placement ofS.annulatusn. sp. in the genusSclerolinumSouthward, 1961. Based oncox1,S.annulatusn. sp. is most closely related to an undescribed siboglinid from off Kushiro in Japan (“Pogonophora” sp. Kushiro-SK-2003). Transmission electron microscopy, microbial16S rRNAamplicon sequencing, phylogenetic reconstruction, and stable isotope analyses together indicate thatS.annulatusn. sp. hosts a single phylotype of sulfur-oxidizing endosymbionts.
Methylphenidate Use and Infectious Diseases in Children With Attention Deficit and Hyperactivity Disorder: A Population-Based Study
Authors: Chen Vincent Chin-Hung , Kao Kai-Liang , Chen Yi-Lung , Wu Shu-I , Lee Min-Jing , Gossop Michael
Published: February 2022

Objective

Children with attention deficit hyperactivity disorder (ADHD) have more visits to the emergency department (ED) due to injuries than those without ADHD. However, no study has investigated whether children with ADHD have more ED visits or hospitalizations due to infectious diseases (IDs) and whether methylphenidate (MPH) treatment may reduce the risk.

Method

The incidence of ID-related ED visits or hospitalizations was defined as the main outcome. The Cox regression and conditional Poisson regression models were calculated to estimate hazard ratios (HRs) in the population level and relative risks for the self-controlled case series design, respectively.

Results

Children with ADHD had higher rates of emergency visits (HR = 1.25, 95% CI: 1.23~1.27) and hospitalizations (HR = 1.28, 95% CI: 1.26~1.31) due to IDs than those without ADHD. In the ADHD subgroup, those who received MPH treatment have a reduced risk of emergency visits (HR = 0.10, 95% CI: 0.09~0.10) and hospitalizations (HR = 0.73, 95% CI: 0.71~0.75), compared to those without treatment. The risk of ID-related emergency visits decreased to 0.21 (95% CI: 0.21~0.22); and hospitalizations decreased to 0.71 (95% CI: 0.69~0.73). Within self-controlled analysis, it is demonstrated that compared with non-MPH exposed period, children with ADHD had significantly decreased risks for infection-related emergency visits (RR = 0.73, 95% CI: 0.68~0.78) or hospitalizations (RR = 0.19, 95% CI: 0.17~0.21) during MPH-exposed periods.

Conclusions and Relevance

This is the first study that reported an increased risk of ID-related healthcare utilizations in children with ADHD compared to those without, and that such risks may be significantly reduced in ADHD children that received MPH treatment.
Expansion of Cyclophyllidea Biodiversity in Rodents of Qinghai-Tibet Plateau and the “Out of Qinghai-Tibet Plateau” Hypothesis of Cyclophyllideans
Authors: Wu Yao-Dong , Dai Guo-Dong , Li Li , Littlewood D. Timothy J. , Ohiolei John Asekhaen , Zhang Lin-Sheng , Guo Ai-Min , Wu Yan-Tao , Ni Xing-Wei , Shumuye Nigus Abebe , Li Wen-Hui , Zhang Nian-Zhang , Fu Bao-Quan , Fu Yong , Yan Hong-Bin , Jia Wan-Zhong
Published: February 2022

The Cyclophyllidea comprises the most species-rich order of tapeworms (Platyhelminthes, Cestoda) and includes species with some of the most severe health impact on wildlife, livestock, and humans. We collected seven Cyclophyllidea specimens from rodents in Qinghai-Tibet Plateau (QTP) and its surrounding mountain systems, of which four specimens in QTP were unsequenced, representing “putative new species.” Their complete mitochondrial (mt) genomes were sequenced and annotated. Phylogenetic reconstruction of partial 28S rDNA, cox1 and nad1 datasets provided high bootstrap frequency support for the categorization of three “putative new species,” assigning each, respectively, to the genera Mesocestoides, Paranoplocephala, and Mosgovoyia, and revealing that some species and families in these three datasets, which contain 291 species from nine families, may require taxonomic revision. The partial 18S rDNA phylogeny of 29 species from Taeniidae provided high bootstrap frequency support for the categorization of the “putative new species” in the genus Hydatigera. Combined with the current investigation, the other three known Taeniidae species found in this study were Taenia caixuepengi, T. crassiceps, and Versteria mustelae and may be widely distributed in western China. Estimates of divergence time based on cox1 + nad1 fragment and mt protein-coding genes (PCGs) showed that the differentiation rate of Cyclophyllidea species was strongly associated with the rate of change in the biogeographic scenarios, likely caused by the uplift of the QTP; i.e., species differentiation of Cyclophyllidea might be driven by host-parasite co-evolution caused by the uplift of QTP. We propose an “out of QTP” hypothesis for the radiation of these cyclophyllidean tapeworms.
Fermentation Profiles, Bacterial Community Compositions, and Their Predicted Functional Characteristics of Grass Silage in Response to Epiphytic Microbiota on Legume Forages
Authors: Wang Siran , Shao Tao , Li Junfeng , Zhao Jie , Dong Zhihao
Published: February 2022

This study aimed to investigate the effect of epiphytic microbiota from alfalfa and red clover on the fermentative products, bacterial community compositions, and their predicted functional characteristics in Italian ryegrass silage. By microbiota transplantation and γ-ray irradiation sterilization, the irradiated Italian ryegrass was treated as follows: (1) sterile distilled water (STIR); (2) epiphytic microbiota on Italian ryegrass (IRIR); (3) epiphytic microbiota on alfalfa (IRAL); and (4) epiphytic microbiota on red clover (IRRC). The irradiated Italian ryegrass was ensiled for 1, 3, 7, 15, 30, and 60 days. STIR had similar chemical components with fresh Italian ryegrass. IRAL had higher lactic acid concentrations [64.0 g/kg dry matter (DM)] than IRIR (22.3 g/kg DM) and IRRC (49.4 g/kg DM) on day 3. IRRC had the lowest lactic acid concentrations (59.7 g/kg DM) and the highest pH (4.64), acetic acid (60.4 g/kg DM), ethanol (20.4 g/kg DM), and ammonia nitrogen (82.6 g/kg DM) concentrations and Enterobacteriaceae [9.51 log10 cfu/g fresh weight (FW)] populations among treatments on day 60. On days 3 and 60, Lactobacillus was dominant in both IRIR (42.2%; 72.7%) and IRAL (29.7%; 91.6%), while Hafnia-Obesumbacterium was predominant in IRRC (85.2%; 48.9%). IRIR and IRAL had lower abundances of “Membrane transport” than IRRC on day 3. IRIR and IRAL had lower abundances of phosphotransacetylase and putative ATP-binding cassette transporter and higher abundances of arginine deiminase on day 3. IRAL had the highest abundance of fructokinase on day 3. Overall, inoculating epiphytic microbiota from different legume forages changed the fermentative products, bacterial community compositions, and their predicted functional characteristics in Italian ryegrass silage. The microbial factors that result in the differences in fermentative profiles between legume forage and grass were revealed. Knowledge regarding the effect of epiphytic microbiota could provide more insights into the improvement of silage quality.
A Cysteine Variant at an Allosteric Site Alters MIF Dynamics and Biological Function in Homo- and Heterotrimeric Assemblies
Authors: Skeens Erin , Pantouris Georgios , Shah Dilip , Manjula Ramu , Ombrello Michael J. , Maluf N. Karl , Bhandari Vineet , Lisi George P. , Lolis Elias J.
Published: February 2022

Macrophage migration inhibitory factor (MIF) is an inflammatory protein with various non-overlapping functions. It is not only conserved in mammals, but it is found in parasites, fish, and plants. Human MIF is a homotrimer with an enzymatic cavity between two subunits with Pro1 as a catalytic base, activates the receptors CD74, CXCR2, and CXCR4, has functional interactions in the cytosol, and is reported to be a nuclease. There is a solvent channel down its 3-fold axis with a recently identified gating residue as an allosteric site important for regulating, to different extents, the enzymatic activity and CD74 binding and signaling. In this study we explore the consequence of converting the allosteric residue Tyr99 to cysteine (Y99C) and characterize its crystallographic structure, NMR dynamics, stability, CD74 function, and enzymatic activity. In addition to the homotrimeric variant, we develop strategies for expressing and purifying a heterotrimeric variant consisting of mixed wild type and Y99C for characterization of the allosteric site to provide more insight.
Identification of UBE2I as a Novel Biomarker in ccRCC Based on a Large-Scale CRISPR-Cas9 Screening Database and Immunohistochemistry
Authors: Li Feng , Lai Li , You Zhijie , Cheng Hui , Guo Guodong , Tang Chenchen , Xu Luyun , Liu Hongxia , Zhong Wenting , Lin Youyu , Wang Qingshui , Lin Yao , Wei Yongbao
Published: February 2022

Background: The genome-wide CRISPR-cas9 dropout screening has emerged as an outstanding approach for characterization of driver genes of tumor growth. The present study aims to investigate core genes related to clear cell renal cell carcinoma (ccRCC) cell viability by analyzing the CRISPR-cas9 screening database DepMap, which may provide a novel target in ccRCC therapy.

Methods: Candidate genes related to ccRCC cell viability by CRISPR-cas9 screening from DepMap and genes differentially expressed between ccRCC tissues and normal tissues from TCGA were overlapped. Weighted gene coexpression network analysis, pathway enrichment analysis, and protein–protein interaction network analysis were applied for the overlapped genes. The least absolute shrinkage and selection operator (LASSO) regression was used to construct a signature to predict the overall survival (OS) of ccRCC patients and validated in the International Cancer Genome Consortium (ICGC) and E-MTAB-1980 database. Core protein expression was determined using immunohistochemistry in 40 cases of ccRCC patients.

Results: A total of 485 essential genes in the DepMap database were identified and overlapped with differentially expressed genes in the TCGA database, which were enriched in the cell cycle pathway. A total of four genes, including UBE2I, NCAPG, NUP93, and TOP2A, were included in the gene signature based on LASSO regression. The high-risk score of ccRCC patients showed worse OS compared with these low-risk patients in the ICGC and E-MTAB-1980 validation cohort. UBE2I was screened out as a key gene. The immunohistochemistry indicated UBE2I protein was highly expressed in ccRCC tissues, and a high-level nuclear translocation of UBE2I occurs in ccRCC. Based on the area under the curve (AUC) values, nuclear UBE2I had the best diagnostic power (AUC = 1). Meanwhile, the knockdown of UBE2I can inhibit the proliferation of ccRCC cells.

Conclusion: UBE2I, identified by CRISPR-cas9 screening, was a core gene-regulating ccRCC cell viability, which accumulated in the nucleus and acted as a potential novel promising diagnostic biomarker for ccRCC patients. Blocking the nuclear translocation of UBE2I may have potential therapeutic value with ccRCC patients.
Admission Random Blood Glucose, Fasting Blood Glucose, Stress Hyperglycemia Ratio, and Functional Outcomes in Patients With Acute Ischemic Stroke Treated With Intravenous Thrombolysis
Authors: Chen Guangyong , Ren Junli , Huang Honghao , Shen Jiamin , Yang Chenguang , Hu Jingyu , Pan Wenjing , Sun Fangyue , Zhou Xinbo , Zeng Tian , Li Shengqi , Yang Dehao , Weng Yiyun
Published: February 2022

Background

Stress hyperglycemia ratio (SHR), calculated as glucose/glycated hemoglobin, has recently been developed for assessing stress hyperglycemia and could provide prognostic information for various diseases. However, calculating SHR using random blood glucose (RBG) drawn on admission or fasting blood glucose (FBG) could lead to different results. This study intends to evaluate the association between SHR and functional outcomes in patients with acute ischemic stroke (AIS) with recombinant tissue plasminogen activator (r-tPA) intravenous thrombolysis.

Methods

Data from 230 patients with AIS following thrombolytic therapy with r-tPA in the Third Affiliated Hospital of Wenzhou Medical University from April 2016 to April 2019 were retrospectively reviewed. SHR1 was defined as [RBG (mmol/L)]/[HbA1c (%)] and SHR2 was defined as [FBG (mmol/L)]/[HbA1c (%)]. The outcomes included early neurological improvement (ENI), poor function defined as a modified Rankin Scale score (mRS) of 3–6, and all-cause death in 3 months. Multivariable logistic regression was performed to estimate the association between SHR and adverse outcomes.

Results

After adjustment for possible confounders, though patients with AIS with higher SHR1 tend to have a higher risk of poor outcome and death and unlikely to develop ENI, these did not reach the statistical significance. In contrast, SHR2 was independently associated with poor functional outcome (per 0.1-point increases: odds ratios (OR) = 1.383 95% CI [1.147–1.668]). Further adjusted for body mass index (BMI), triglyceride-glucose index (TyG), and diabetes slightly strengthen the association between SHR (both 1 and 2) and adverse outcomes. In subgroup analysis, elevated SHR1 is associated with poor functional outcomes (per 0.1-point increases: OR = 1.246 95% CI [1.041–1.492]) in non-diabetic individuals and the association between SHR2 and the poor outcomes was attenuated in non-cardioembolic AIS.

Conclusion

SHR is expected to replace random or fasting glucose concentration as a novel generation of prognostic indicator and a potential therapeutic target.
Adaptive Interlimb Coordination Mechanism for Hexapod Locomotion Based on Active Load Sensing
Authors: Fukuhara Akira , Suda Wataru , Kano Takeshi , Kobayashi Ryo , Ishiguro Akio
Published: February 2022

Insects can flexibly coordinate their limbs to adapt to various locomotor conditions, e.g., complex environments, changes in locomotion speed, and leg amputation. An interesting aspect of insect locomotion is that the gait patterns are not necessarily stereotypical but are often highly variable, e.g., searching behavior to obtain stable footholds in complex environments. Several previous studies have focused on the mechanism for the emergence of variable limb coordination patterns. However, the proposed mechanisms are complicated and the essential mechanism underlying insect locomotion remains elusive. To address this issue, we proposed a simple mathematical model for the mechanism of variable interlimb coordination in insect locomotion. The key idea of the proposed model is “decentralized active load sensing,” wherein each limb actively moves and detects the reaction force from the ground to judge whether it plays a pivotal role in maintaining the steady support polygon. Based on active load sensing, each limb stays in the stance phase when the limb is necessary for body support. To evaluate the proposed model, we conducted simulation experiments using a hexapod robot. The results showed that the proposed simple mechanism allows the hexapod robot to exhibit typical gait patterns in response to the locomotion speed. Furthermore, the proposed mechanism improves the adaptability of the hexapod robot for leg amputations and lack of footholds by changing each limb's walking and searching behavior in a decentralized manner based on the physical interaction between the body and the environment.
Myoelectric Arm Orthosis in Motor Learning-Based Therapy for Chronic Deficits After Stroke and Traumatic Brain Injury
Authors: Pundik Svetlana , McCabe Jessica , Skelly Margaret , Salameh Ahlam , Naft Jonathan , Chen Zhengyi , Tatsuoka Curtis , Fatone Stefania
Published: February 2022

Background

Technologies that enhance motor learning-based therapy and are clinically deployable may improve outcome for those with neurological deficits. The MyoPro™ is a customized myoelectric upper extremity orthosis that utilizes volitionally generated weak electromyographic signals from paretic muscles to assist movement of an impaired arm. Our purpose was to evaluate MyoPro as a tool for motor learning-based therapy for individuals with chronic upper limb weakness.

Methods

This was a pilot study of thirteen individuals with chronic moderate/severe arm weakness due to either stroke (n = 7) or TBI (n = 6) who participated in a single group interventional study consisting of 2 phases. The in-clinic phase included 18 sessions (2x per week, 27hrs of face-to-face therapy) plus a home exercise program. The home phase included practice of the home exercise program. The study did not include a control group. Outcomes were collected at baseline and at weeks 3, 5, 7, 9, 12, 15, and 18. Statistics included mixed model regression analysis.

Results

Statistically significant and clinically meaningful improvements were observed on Fugl-Meyer (+7.5 points). Gains were seen at week 3, increased further through the in-clinic phase and were maintained during the home phase. Statistically significant changes in Modified Ashworth Scale, Range of Motion, and Chedoke Arm and Hand Activity Inventory were seen early during the in-clinic phase. Orthotic and Prosthetic User's Survey demonstrated satisfaction with the device throughout study participation. Both stroke and TBI participants responded to the intervention.

Conclusions

Use of MyoPro in motor learning-based therapy resulted in clinically significant gains with a relatively short duration of in-person treatment. Further studies are warranted.

Clinical Trial Registration

www.ClinicalTrials.gov, identifier: NCT03215771.