bluuebunny/crossref_metadata_2025 huggingface dataset:
Lipoedema as a Social Problem. A Scoping Review
Authors: Czerwińska Monika , Ostrowska Paulina , Hansdorfer-Korzon Rita
Published: September 2021

(1) Background: Lipoedema is a disease characterized by excessive bilateral and symmetrical accumulation of subcutaneous tissue in the lower extremities. It is a poorly understood condition, and low awareness of its existence often leads to incorrect diagnosis Initially, lipoedema was considered to be completely independent of lifestyle Currently, however, more and more cases of the coexistence of lipoedema and obesity are described in the literature as additionally affecting the severity of the disease The aim of the review is to present lipoedema as a social problem. (2) Methods: Materials on lipoedema in the social context were selected from 2018–2021. The PRISMA-Scr checklist was used in the review. (3) Results: Research has shown that more than 3/4 of patients with lipoedema are also overweight or obese. Patients with lipoedema have many comorbidities, and their presence negatively affects the quality of life. The quality of life in patients with lipoedema is lower than in healthy patients. (4) Conclusions: The number of studies available on lipoedema is low. Obesity is common in patients with lipoedema. Mental disorders increase the level of experienced pain. Lipoedema significantly reduces quality of life. A healthy lifestyle in patients with lipoedema could be helpful for prevention of complications and disability.
Caregiver Willingness to Vaccinate Their Children against COVID-19 after Adult Vaccine Approval
Authors: Goldman Ran D. , Krupik Danna , Ali Samina , Mater Ahmed , Hall Jeanine E. , Bone Jeffrey N. , Thompson Graham C. , Yen Kenneth , Griffiths Mark A. , Klein Adi , Klein Eileen J. , Brown Julie C. , Mistry Rakesh D. , Gelernter Renana ,   on behalf of the International COVID-19 Parental Attitude Study (COVIPAS) Group
Published: September 2021

Vaccines against COVID-19 are likely to be approved for children under 12 years in the near future. Understanding vaccine hesitancy in parents is essential for reaching herd immunity. A cross-sectional survey of caregivers in 12 emergency departments (ED) was undertaken in the U.S., Canada, and Israel. We compared reported willingness to vaccinate children against COVID-19 with an initial survey and post-adult COVID-19 vaccine approval. Multivariable logistic regression models were performed for all children and for those <12 years. A total of 1728 and 1041 surveys were completed in phases 1 and 2, respectively. Fewer caregivers planned to vaccinate against COVID-19 in phase 2 (64.5% and 59.7%, respectively; p = 0.002). The most significant positive predictor of willingness to vaccinate against COVID-19 was if the child was vaccinated per recommended local schedules. Fewer caregivers plan to vaccinate their children against COVID-19, despite vaccine approval for adults, compared to what was reported at the peak of the pandemic. Older caregivers who fully vaccinated their children were more likely to adopt vaccinating children. This study can inform target strategy design to implement adherence to a vaccination campaign.
Pedestrianization Impacts on Air Quality Perceptions and Environment Satisfaction: The Case of Regenerated Streets in Downtown Seoul
Authors: Kim UnHyo , Lee Jeongwoo , He Sylvia Y.
Published: September 2021

Previous studies have investigated the increased volume of pedestrians to establish success rates of the pedestrian-friendly policy after a street redesign intervention. However, few studies have focused on the effect of street regeneration on air quality perception and user satisfaction. The influence of the physical environment on street vitality may vary, depending on area context and regional factors. A comprehensive understanding of effective interventions could increase pedestrians’ satisfaction with their walking environment. This study examines the effect of pedestrianization on individuals’ air quality perception and satisfaction, based on three regenerated streets in Seoul, Korea. We analyzed data from 672 questionnaires administered after the pedestrianization project. We used a subset of variables in a binary logistic regression model to understand general determinants of user satisfaction toward their walking environment. Our case study contributes to the verification of pedestrianization effects on air quality perceptions. Results show that overall satisfaction could be acquired through positive perceptions of air quality, as achieved through pedestrianization of streets. Moreover, pedestrian satisfaction varies according to the purpose, activities and health-related behaviors and attitudes. The interrelationships between environmental health, activity, satisfaction and quality of life provide design insights to consider when implementing pedestrianization projects in the future.
Gut Microbiota in Lung Cancer: Where Do We Stand?
Authors: Georgiou Konstantinos , Marinov Blagoi , Farooqi Ammad Ahmad , Gazouli Maria
Published: September 2021

The gut microbiota (GM) is considered to constitute a powerful “organ” capable of influencing the majority of the metabolic, nutritional, physiological, and immunological processes of the human body. To date, five microbial-mediated mechanisms have been revealed that either endorse or inhibit tumorigenesis. Although the gastrointestinal and respiratory tracts are distant physically, they have common embryonic origin and similarity in structure. The lung microbiota is far less understood, and it is suggested that the crosslink between the human microbiome and lung cancer is a complex, multifactorial relationship. Several pathways linking their respective microbiota have reinforced the existence of a gut–lung axis (GLA). Regarding implications of specific GM in lung cancer therapy, a few studies showed that the GM considerably affects immune checkpoint inhibitor (ICI) therapy by altering the differentiation of regulatory T cells and thus resulting in changes in immunomodulation mechanisms, as discovered by assessing drug metabolism directly and by assessing the host immune modulation response. Additionally, the GM may increase the efficacy of chemotherapeutic treatment in lung cancer. The mechanism underlying the role of the GLA in the pathogenesis and progression of lung cancer and its capability for diagnosis, manipulation, and treatment need to be further explored.
Kinases of the Focal Adhesion Complex Contribute to Cardiomyocyte Specification
Authors: Robert Sacha , Flowers Marcus , Ogle Brenda M.
Published: September 2021

Differentiation of pluripotent stem cells to cardiomyocytes is influenced by culture conditions including the extracellular matrices or similar synthetic scaffolds on which they are grown. However, the molecular mechanisms that link the scaffold with differentiation outcomes are not fully known. Here, we determined by immunofluorescence staining and mass spectrometry approaches that extracellular matrix (ECM) engagement by mouse pluripotent stem cells activates critical components of canonical wingless/integrated (Wnt) signaling pathways via kinases of the focal adhesion to drive cardiomyogenesis. These kinases were found to be differentially activated depending on type of ECM engaged. These outcomes begin to explain how varied ECM composition of in vivo tissues with development and in vitro model systems gives rise to different mature cell types, having broad practical applicability for the design of engineered tissues.
Placental Expression of Bile Acid Transporters in Intrahepatic Cholestasis of Pregnancy
Authors: Ontsouka Edgar , Epstein Alessandra , Kallol Sampada , Zaugg Jonas , Baumann Marc , Schneider Henning , Albrecht Christiane
Published: September 2021

Intrahepatic cholestasis of pregnancy (ICP) is a pregnancy-related condition characterized by increased maternal circulating bile acids (BAs) having adverse fetal effects. We investigated whether the human placenta expresses specific regulation patterns to prevent fetal exposition to harmful amounts of BAs during ICP. Using real-time quantitative PCR, we screened placentae from healthy pregnancies (n = 12) and corresponding trophoblast cells (n = 3) for the expression of 21 solute carriers and ATP-binding cassette transporter proteins, all acknowledged as BA- and/or cholestasis-related genes. The placental gene expression pattern was compared between healthy women and ICP patients (n = 12 each). Placental SLCO3A1 (OATP3A1) gene expression was significantly altered in ICP compared with controls. The other 20 genes, including SLC10A2 (ASBT) and EPHX1 (EPOX, mEH) reported for the first time in trophoblasts, were comparably abundant in healthy and ICP placentae. ABCG5 was undetectable in all placentae. Placental SLC10A2 (ASBT), SLCO4A1 (OATP4A1), and ABCC2 mRNA levels were positively correlated with BA concentrations in ICP. Placental SLC10A2 (ASBT) mRNA was also correlated with maternal body mass index. We conclude that at the transcriptional level only a limited response of BA transport systems is found under ICP conditions. However, the extent of the transcriptional response may also depend on the severity of the ICP condition and the magnitude by which the maternal BA levels are increased.
Adaption and Degradation Strategies of Methylotrophic 1,4-Dioxane Degrading Strain Xanthobacter sp. YN2 Revealed by Transcriptome-Scale Analysis
Authors: Wang Yingning , Ma Fang , Yang Jixian , Guo Haijuan , Su Delin , Yu Lan
Published: September 2021

Biodegradation of 1,4-dioxane (dioxane) contamination has gained much attention for decades. In our previous work, we isolated a highly efficient dioxane degrader, Xanthobacter sp. YN2, but the underlying mechanisms of its extraordinary degradation performance remained unresolved. In this study, we performed a comparative transcriptome analysis of YN2 grown on dioxane and citrate to elucidate its genetic degradation mechanism and investigated the transcriptomes of different dioxane degradation stages (T0, T24, T48). We also analyzed the transcriptional response of YN2 over time during which the carbon source switched from citrate to dioxane. The results indicate that strain YN2 was a methylotroph, which provides YN2 a major advantage as a pollutant degrader. A large number of genes involved in dioxane metabolism were constitutively expressed prior to dioxane exposure. Multiple genes related to the catabolism of each intermediate were upregulated by treatment in response to dioxane. Glyoxylate metabolism was essential during dioxane degradation by YN2, and the key intermediate glyoxylate was metabolized through three routes: glyoxylate carboligase pathway, malate synthase pathway, and anaplerotic ethylmalonyl–CoA pathway. Genes related to quorum sensing and transporters were significantly upregulated during the early stages of degradation (T0, T24) prior to dioxane depletion, while the expression of genes encoding two-component systems was significantly increased at late degradation stages (T48) when total organic carbon in the culture was exhausted. This study is the first to report the participation of genes encoding glyoxalase, as well as methylotrophic genes xoxF and mox, in dioxane metabolism. The present study reveals multiple genetic and transcriptional strategies used by YN2 to rapidly increase biomass during growth on dioxane, achieve high degradation efficiency and tolerance, and adapt to dioxane exposure quickly, which provides useful information regarding the molecular basis for efficient dioxane biodegradation.
Phage Therapy as a Focused Management Strategy in Aquaculture
Authors: Ramos-Vivas José , Superio Joshua , Galindo-Villegas Jorge , Acosta Félix
Published: September 2021

Therapeutic bacteriophages, commonly called as phages, are a promising potential alternative to antibiotics in the management of bacterial infections of a wide range of organisms including cultured fish. Their natural immunogenicity often induces the modulation of a variated collection of immune responses within several types of immunocytes while promoting specific mechanisms of bacterial clearance. However, to achieve standardized treatments at the practical level and avoid possible side effects in cultivated fish, several improvements in the understanding of their biology and the associated genomes are required. Interestingly, a particular feature with therapeutic potential among all phages is the production of lytic enzymes. The use of such enzymes against human and livestock pathogens has already provided in vitro and in vivo promissory results. So far, the best-understood phages utilized to fight against either Gram-negative or Gram-positive bacterial species in fish culture are mainly restricted to the Myoviridae and Podoviridae, and the Siphoviridae, respectively. However, the current functional use of phages against bacterial pathogens of cultured fish is still in its infancy. Based on the available data, in this review, we summarize the current knowledge about phage, identify gaps, and provide insights into the possible bacterial control strategies they might represent for managing aquaculture-related bacterial diseases.
Implementing Big Data Analytics in Marketing Departments: Mixing Organic and Administered Approaches to Increase Data-Driven Decision Making
Authors: Johnson Devon S. , Sihi Debika , Muzellec Laurent
Published: September 2021

This study examines the experience of marketing departments to become fully data-driven decision-making organizations. We evaluate an organic approach of departmental sensemaking and an administered approach by which top management increase the influence of analytics skilled employees. Data collection commenced with 15 depth interviews of marketing and analytics professionals in the US and Europe involved in the implementation of big data analytics (BDA) and was followed by a survey data of 298 marketing and analytics middle management professionals at United States based firms. The survey data supports the logic that BDA sensemaking is initiated by top management and is comprised of four primary activities: external knowledge acquisition, improving digitized data quality, big data analytics experimentation and big data analytics information dissemination. Top management drives progress toward data-driven decision-making by facilitating sensemaking and by increasing the influence of BDA skilled employees. This study suggests that while a shift toward enterprise analytics increases the quality of resource available to the marketing department, this approach could stymie the quality of marketing insights gained from BDA. This study presents a model of how to improve the quality of marketing insights and improve data-driven decision-making.
Notes on the Morphology and Systematic Position of Archaeolus Lin, 1986, from the Jurassic of South China (Coleoptera: Elateroidea)
Authors: Li Yan-Da , Kundrata Robin , Huang Di-Ying , Cai Chen-Yang
Published: September 2021

The morphology of the Jurassic fossil Archaeolus funestus Lin, 1986, which was previously placed in the extinct click-beetle subfamily Protagrypninae (Coleoptera: Elateridae), is revised based on a re-examination of the type specimen. The validity of Protagrypninae is discussed and further questioned, partly based on the newly observed characters in A. funestus, including the surface sculpture of the mesoventrite. A possible Throscidae affinity of monotypic Archaeolus Lin, 1986, as suggested in a recent study, is further critically reviewed.
An Assessment of the Performance of the Secure Remote Update Protocol in Simulated Real-World Conditions
Authors: Poulter Andrew John , Cox Simon J.
Published: September 2021

This paper assesses the relative performance of the MQTT protocol in comparison to the Secure Remote Update Protocol (SRUP) in a number of simulated real-world conditions, and describes an experiment that has been conducted to measure the processing delay associated with the use of the more secure protocol. Experimental measurements for power consumption of the devices and the size of comparable TCP packets were also made. Analysis shows that the use of the SRUP protocol added an additional processing delay of between 42.92 ms and 51.60 ms—depending on the specific hardware in use. There was also shown to be a 55.47% increase in power consumption when running the secure SRUP protocol, compared with an MQTT implementation.
Prognostic Role of Chronic Rhinosinusitis in Acute Ischemic Stroke Patients Undergoing Mechanical Thrombectomy
Authors: Puz Przemysław , Stryjewska-Makuch Grażyna , Żak Amadeusz , Rybicki Wiktor , Student Sebastian , Lasek-Bal Anetta
Published: September 2021

Background: The aim of the study was to assess the relevance of chronic rhinosinusitis (CRS) CT features to the efficacy of mechanical thrombectomy (MT) in patients with acute ischemic stroke. Methods: This study included 311 patients qualified for MT in whom the CRS features were assessed based on a CT scan, according to the Lund-Mackay (L-M) score. Clinical, inflammatory parameters, patients neurological (NIHSS) and functional status (mRS), and recanalisation efficacy (TICI) were compared between patients with mild lesions (L-M score 0–3 points)-group 1, and patients with more severe lesions (L–M score 4–24)-group 2. Results: There was a significant difference in the NIHSS on day seven after stroke onset-10 points in group 1 and 14 points in group 2, p = 0.02. NIHSS ≤ 6 points on day seven was found in 41.9% of patients in group 1, and in 27.5% in group 2, p = 0.042. There were no significant differences in mRS score and in the TICI score. L-M score, lipid abnormalities and CRP were factors associated with NIHSS ≥ 7 points on day 7. Conclusions: The CT features of CRS may be used as a prognostic tool for early prognosis assessment in stroke patients.
Advances in Cardiac Development and Regeneration Using Zebrafish as a Model System for High-Throughput Research
Authors: Francoeur Nicholas , Sen Rwik
Published: September 2021

Heart disease is the leading cause of death in the United States and worldwide. Understanding the molecular mechanisms of cardiac development and regeneration will improve diagnostic and therapeutic interventions against heart disease. In this direction, zebrafish is an excellent model because several processes of zebrafish heart development are largely conserved in humans, and zebrafish has several advantages as a model organism. Zebrafish transcriptomic profiles undergo alterations during different stages of cardiac development and regeneration which are revealed by RNA-sequencing. ChIP-sequencing has detected genome-wide occupancy of histone post-translational modifications that epigenetically regulate gene expression and identified a locus with enhancer-like characteristics. ATAC-sequencing has identified active enhancers in cardiac progenitor cells during early developmental stages which overlap with occupancy of histone modifications of active transcription as determined by ChIP-sequencing. CRISPR-mediated editing of the zebrafish genome shows how chromatin modifiers and DNA-binding proteins regulate heart development, in association with crucial signaling pathways. Hence, more studies in this direction are essential to improve human health because they answer fundamental questions on cardiac development and regeneration, their differences, and why zebrafish hearts regenerate upon injury, unlike humans. This review focuses on some of the latest studies using state-of-the-art technology enabled by the elegant yet simple zebrafish.
The Associations between Asymmetric Handgrip Strength and Chronic Disease Status in American Adults: Results from the National Health and Nutrition Examination Survey
Authors: Klawitter Lukus , Bradley Adam , Hackney Kyle , Tomkinson Grant , Christensen Bryan , Kraemer William , McGrath Ryan
Published: September 2021

This study examined the associations between asymmetric handgrip strength (HGS) and multimorbidity in American adults. Secondary analyses of data from persons aged at least 40 years from the 2011–2012 and 2013–2014 waves of the National Health and Nutrition Examination Survey were conducted. A handheld dynamometer collected HGS on each hand and persons with a strength imbalance >10% between hands were classified as having asymmetric HGS. Adults with the presence of ≥2 of the following conditions had multimorbidity: cardiovascular disease, chronic obstructive pulmonary disease, chronic kidney disease, asthma, arthritis, cancer, obesity, stroke, hypertension, high cholesterol, and diabetes. Of the n = 3483 participants included, n = 2700 (77.5%) had multimorbidity. A greater proportion of adults with multimorbidity had HGS asymmetry (n = 1234 (45.7%)), compared to persons living without multimorbidity (n = 314 (40.1%); p < 0.05). Relative to individuals without asymmetry, adults with asymmetric HGS had 1.31 (95% confidence interval (CI): 1.03–1.67) greater odds for multimorbidity. Moreover, persons with HGS asymmetry had 1.22 (CI: 1.04–1.44) greater odds for accumulating morbidities. Asymmetric strength, as another indicator of diminished muscle function, is linked to chronic morbidity status. Healthcare providers should recommend healthy behaviors for reducing asymmetries to improve muscle function and mitigate morbidity risk after completing asymmetry screenings.
Effectiveness of On-Court Resistive Warm-Ups on Change of Direction Speed and Smash Velocity during a Simulated Badminton Match Play in Well-Trained Players
Authors: Chua Man Tong , Chow Kin Ming , Lum Danny , Tay Andrew Wei Han , Goh Wan Xiu , Ihsan Mohammed , Aziz Abdul Rashid
Published: September 2021

In badminton, power production can be enhanced through the fundamental practice of a dynamic warm-up with resistance conditioning activity to induce a post-activation performance enhancement (PAPE) effect. The use of heavy resistance exercise in the form of heavy weights to induce PAPE during competition is not logistically practical in the badminton arena. Thus, there is a need to investigate the use of easily available alternative preconditioning stimuli to induce a similar potentiating effect in badminton-specific performance. This study adopted a repeated-measures design of three warm-up conditions: control (CON), weighted wearable resistance (WWR), and resistance band variable resistance (BVR). Fourteen badminton players from the national training squad (11 males, 3 females, age 18 ± 1 y) completed the experimental sessions in random order. Change of direction speed (CODS) and smash velocity (SV) tests were performed at five timepoints—baseline test after the warm-up and at the end of each of the four exercise blocks of a simulated match play protocol. CODS was significantly faster under the two resistance warm-up conditions (WWR and BVR) compared to the CON condition at baseline (−0.2 s ± 0.39 and −0.2 s ± 0.46, p = 0.001 and 0.03, g = 0.47 and 0.40, respectively), but there were no differences at the other timepoints (all p > 0.05). SV was significantly faster for all the four exercise blocks than at baseline under all three warm-up conditions (p = 0.02), but there were no differences in SV between the three warm-up conditions across all the five measured timepoints (p = 0.15). In conclusion, implementing resistance (~10% body weight) in sport-specific plyometric exercises using WWR or BVR during warm-up routines may induce PAPE effects on the change of direction speed but not smash velocity, in well-trained badminton players, as compared with the same warm-up exercises using bodyweight (i.e., CON condition). The positive effects of CODS were, however, observed only at the start of the match and possibly lasted for up to between 5 and 10 min of match play.
An Experimental Pricing Framework for E-Commerce
Authors: Vatsa Puneet , Ma Wanglin , Zhou Xiaoshi
Published: September 2021

Characterizing the demand curve of products is important for pricing them optimally. However, in deriving empirical demand curves, econometricians have to contend with identification issues. Furthermore, theoretical demand curves derived using standard economic theory are divorced from empirical realities: firms rarely have information on customers’ budget constraints; theoretical utility functions are seldom derived empirically. Recognizing these issues, we propose an experimental approach for determining a product’s demand curve and, in turn, its profit-maximizing price in online environments. The proposed approach yields precise estimates and is quick and inexpensive to implement.
The Heterogeneous Influence of Infrastructure Construction on China’s Urban Green and Smart Development—The Threshold Effect of Urban Scale
Authors: Xu Lingyan , Wang Dandan , Du Jianguo
Published: September 2021

The construction of green and smart cities is an important approach to enhancing the level of high-quality development and modern governance, in which infrastructure construction is the antecedent condition. From the perspective of green total factor productivity (GTFP), this paper adopts the SBM–GML (Slack-Based Model and Global Malmquist–Luenberger) index to measure the urban green and smart development level (GSDL) considering smart input–output factors. Based on the panel data of China’s 223 prefecture-level cities from 2005 to 2018, the dynamic impacts, temporal, and spatial differences of energy, transportation, and telecommunication infrastructure construction on the urban GSDL are discussed, and the threshold effects of urban scale are tested. The following conclusions are drawn: (1) On the whole, energy infrastructure inhibits the urban GSDL, while transportation and telecommunication infrastructures significantly promote it. There are distinct spatial and temporal characteristics among the impacts of these three infrastructures on the urban GSDL, in which the facilitating role of transportation and telecommunication infrastructures are further enhanced during the period of 2013–2018. Furthermore, the impacts of these three infrastructures on the urban GSDL all show “U” shape in terms of non-linearity. (2) Economic development level and industrial structure have significant positive effects on the urban GSDL, whereas human capital only has positive effect in the northeast and southwest regions, and government scale shows no positive impact yet. (3) There is a single threshold for the impact of urban scale on these three infrastructures, among which the impacts of energy and transportation infrastructures on the urban GSDL remain consistent before and after the threshold, while the impact of telecommunication infrastructure on the urban GSDL varies from having no significance to being positive when crossing the threshold. Thus, capital investment for infrastructure construction should be further allocated reasonably, the positive potential of human capital should be fully released, and the urban scale should be appropriately controlled in the future.
The Ginsenoside Rg1 Rescues Mitochondrial Disorders in Aristolochic Acid-Induced Nephropathic Mice
Authors: Chou Chu-Kuang , Huang Yu-Shen , Lin Pei-Yu , Imai Kazuhiro , Chen Shih-Ming , Lee Jen-Ai
Published: September 2021

Chronic exposure to aristolochic acid (AA) leads to renal interstitial fibrosis and nephropathy. In this study, we aimed to investigate the renoprotective effects of Panax ginseng extract (GE) and ginsenoside saponin (GS) on AA-induced nephropathy (AAN) in mice. Eighty female C3H/He mice were randomly divided into eight groups, including normal; AA (3 μg/mL for 56 days); AA with GE (125, 250, or 500 mg/kg/d for 14 days); and AA with important GE ingredients, Rg1, Rb1, or Rd (5 mg/kg/d for 14 days). Compared with the AA group, renal injuries were significantly decreased in the GE (250 mg/kg/d), Rb1, and Rg1 treatment groups. Rg1 exhibited the best renoprotection among all GS-treated groups. There were 24 peaks significantly altered among normal, AA, and AA + Rg1 groups, and four mitochondrial proteins were identified, including acyl-CoA synthetase medium-chain family member 2, upregulated during skeletal muscle growth 5 (Usmg5), mitochondrial aconitase 2 (ACO2), and cytochrome c oxidase subunit Va preprotein (COX5a). We demonstrated for the first time that the AAN mechanism and renoprotective effects of Rg1 are associated with expression of mitochondrial proteins, especially ACO2, Usmg5, and COX5a.
Strengthening Properties and Wear Resistance of the Cu-xNi-yCo-Cr-Si Alloy by Varying Ni/Co and Zr Addition
Authors: Avila-Salgado Denis Ariel , Juárez-Hernández Arturo , Cabral-Miramontes José , Camacho-Martínez José Luis
Published: September 2021

This work examines the effect of nickel (Ni) and cobalt (Co) concentrations on wear resistance behavior of Cu-xNi-yCo-Cr-Si (x = 8−9 wt%, y = 1−2 wt%) and the zirconium (Zr) addition, in order to obtain an alloy with similar or better properties than the C17510 alloy. In this study, hardness and wear resistance mechanism behavior were associated with the microstructure evolution of these alloys with different aging strengthening conditions. Different heat treatments (HT) were applied to three alloys Cu-8Ni-2Co-1.6Cr-2Si wt% (L1), Cu-8.5Ni-1.5Co-1.6Cr-2Si wt% (L2) and Cu-9Ni-1Co-1.6Cr-2Si-0.18Fe-0.3Zr wt% (L3), the properties obtained were compared with C17510 alloy. Specimens were characterized by different metallographic techniques; the microstructural characterization was made with scanning electron microscopy (SEM) and revealed regions of dendritic morphologies. The solidification sequence is primary Cu dendrites and some phases in the interdendritic regions. The solution heat treatment decreased (ST) the Hardness Rockwell B (HRB) and wear rate resistance, and there was an increase with the first aging heat treatment (HT1) for each alloy. The most outstanding combination of properties was found for Cu-8.5Ni-1.5Co-1.6Cr-2Si wt% alloy (L2C3) with 100 ± 1 HRB and specific wear rate resistance of 1.2 × 10−4 mm3/Nm, after the first aging heat treatment.
An Assessment of the Application of Private Aggregation of Ensemble Models to Sensible Data
Authors: Yovine Sergio , Mayr Franz , Sosa Sebastián , Visca Ramiro
Published: September 2021

This paper explores the use of Private Aggregation of Teacher Ensembles (PATE) in a setting where students have their own private data that cannot be revealed as is to the ensemble. We propose a privacy model that introduces a local differentially private mechanism to protect student data. We implemented and analyzed it in case studies from security and health domains, and the result of the experiment was twofold. First, this model does not significantly affecs predictive capabilities, and second, it unveiled interesting issues with the so-called data dependency privacy loss metric, namely, high variance and values.
Temperature Impacts on Endurance and Read Disturbs in Charge-Trap 3D NAND Flash Memories
Authors: Chen Fei , Chen Bo , Lin Hongzhe , Kong Yachen , Liu Xin , Zhan Xuepeng , Chen Jiezhi
Published: September 2021

Temperature effects should be well considered when designing flash-based memory systems, because they are a fundamental factor that affect both the performance and the reliability of NAND flash memories. In this work, aiming to comprehensively understanding the temperature effects on 3D NAND flash memory, triple-level-cell (TLC) mode charge-trap (CT) 3D NAND flash memory chips were characterized systematically in a wide temperature range (−30~70 °C), by focusing on the raw bit error rate (RBER) degradation during program/erase (P/E) cycling (endurance) and frequent reading (read disturb). It was observed that (1) the program time showed strong dependences on the temperature and P/E cycles, which could be well fitted by the proposed temperature-dependent cycling program time (TCPT) model; (2) RBER could be suppressed at higher temperatures, while its degradation weakly depended on the temperature, indicating that high-temperature operations would not accelerate the memory cells’ degradation; (3) read disturbs were much more serious at low temperatures, while it helped to recover a part of RBER at high temperatures.
MEMS-Based Integrated Triaxial Electrochemical Seismometer
Authors: Qi Wenjie , Liu Bowen , Liang Tian , Chen Jian , Chen Deyong , Wang Junbo
Published: September 2021

This paper presents a micro-electromechanical systems (MEMS)-based integrated triaxial electrochemical seismometer, which can detect three-dimensional vibration. By integrating three axes, the integrated triaxial electrochemical seismometer is characterized by small volume and high symmetry. The numerical simulation results inferred that the integrated triaxial electrochemical seismometer had excellent independence among three axes. Based on the experimental results, the integrated triaxial electrochemical seismometer had the advantage of small axial crosstalk and could detect vibration in arbitrary directions. Furthermore, compared with the uniaxial electrochemical seismometer, the integrated triaxial electrochemical seismometer had similar sensitivity curves ranging from 0.01 to 100 Hz. In terms of random ground motion response, high consistencies between the developed integrated triaxial electrochemical seismometer and the uniaxial electrochemical seismometer could be easily observed, which indicated that the developed integrated triaxial electrochemical seismometer produced comparable noise levels to those of the uniaxial electrochemical seismometer. These results validated the performance of the integrated triaxial electrochemical seismometer, which has a good prospect in the field of deep geophysical exploration and submarine seismic monitoring.
Optimization of Copper Electroforming Process Parameters Based on Double Hidden Layer BP Neural Network
Authors: Ji Feng , Chen Chao , Zhao Yongfei , Min Byungwon
Published: September 2021

In order to optimize the pulse electroforming copper process, a double hidden layer BP (back propagation) neural network was constructed. Through sample training, the mapping relationship between electroforming copper process conditions and target properties was accurately established, and the prediction of microhardness and tensile strength of the electroforming layer in the pulse electroforming copper process was realized. The predicted results were verified by electrodeposition copper test in copper pyrophosphate solution system with pulse power supply. The results show that the microhardness and tensile strength of copper layer predicted by “3-4-3-2” structure double hidden layer neural network are very close to the experimental values, and the relative error is less than 2.82%. In the parameter range, the microhardness of copper layer is between 100.3~205.6 MPa and the tensile strength is between 165~485 MPa. When the microhardness and tensile strength are optimal, the corresponding range of optimal parameters are as follows: current density is 2–3 A·dm−2, pulse frequency is 1.5–2 kHz and pulse duty cycle is 10–20%.
Development of GaN HEMTs Fabricated on Silicon, Silicon-on-Insulator, and Engineered Substrates and the Heterogeneous Integration
Authors: Hsu Lung-Hsing , Lai Yung-Yu , Tu Po-Tsung , Langpoklakpam Catherine , Chang Ya-Ting , Huang Yu-Wen , Lee Wen-Chung , Tzou An-Jye , Cheng Yuh-Jen , Lin Chun-Hsiung , Kuo Hao-Chung , Chang Edward Yi
Published: September 2021

GaN HEMT has attracted a lot of attention in recent years owing to its wide applications from the high-frequency power amplifier to the high voltage devices used in power electronic systems. Development of GaN HEMT on Si-based substrate is currently the main focus of the industry to reduce the cost as well as to integrate GaN with Si-based components. However, the direct growth of GaN on Si has the challenge of high defect density that compromises the performance, reliability, and yield. Defects are typically nucleated at the GaN/Si heterointerface due to both lattice and thermal mismatches between GaN and Si. In this article, we will review the current status of GaN on Si in terms of epitaxy and device performances in high frequency and high-power applications. Recently, different substrate structures including silicon-on-insulator (SOI) and engineered poly-AlN (QST®) are introduced to enhance the epitaxy quality by reducing the mismatches. We will discuss the development and potential benefit of these novel substrates. Moreover, SOI may provide a path to enable the integration of GaN with Si CMOS. Finally, the recent development of 3D hetero-integration technology to combine GaN technology and CMOS is also illustrated.
Air-Filled Microbubbles Based on Albumin Functionalized with Gold Nanocages and Zinc Phthalocyanine for Multimodal Imaging
Authors: Maksimova Elizaveta A. , Barmin Roman A. , Rudakovskaya Polina G. , Sindeeva Olga A. , Prikhozhdenko Ekaterina S. , Yashchenok Alexey M. , Khlebtsov Boris N. , Solovev Alexander A. , Huang Gaoshan , Mei Yongfeng , Kanti Dey Krishna , Gorin Dmitry A.
Published: September 2021

Microbubbles are intravascular contrast agents clinically used in diagnostic sonography, echocardiography, and radiology imaging applications. However, up to date, the idea of creating microbubbles with multiple functionalities (e.g., multimodal imaging, photodynamic therapy) remained a challenge. One possible solution is the modification of bubble shells by introducing specific compounds responsible for such functions. In the present work, air-core microbubbles with the shell consisting of bovine serum albumin, albumin-coated gold nanocages, and zinc phthalocyanine were prepared using the sonication method. Various physicochemical parameters such as stability over time, size, and concentration were investigated to prove the potential use of these microbubbles as contrast agents. This work shows that hybrid microbubbles have all the necessary properties for multimodal imaging (ultrasound, raster-scanning microscopy, and fluorescence tomography), which demonstrate superior characteristics for potential theranostic and related biomedical applications.
Moderate Hypothermia Has the Potential to Reveal the Dominant/Submissive Relationship in a Co-Culture System Consisting of Osteoblasts and Endothelial Cells
Authors: Inomata Kouki , Honda Michiyo
Published: September 2021

Microvessels in bone are indispensable for maintaining bone homeostasis based on a dynamic remodeling system. In cell-based tissue engineering, vascularization into the regenerative bone is a key strategy to avoid hypoxia and necrosis around re-implanted tissues. Previous studies have shown that direct contact between osteoblasts and endothelial cells stimulates differentiation of both cell types. However, no studies have revealed the dominant/submissive relationship. In the present study, we examined the effect of hypothermia on monoculture and co-culture to assess which cells tightly coordinated osteogenesis and angiogenesis in the co-culture system. As for osteoblasts, exposure to hypothermia suppressed cellular proliferation, migration, and differentiation. Evaluation of the behavior of endothelial cells showed that hypothermia should not affect basic functions such as proliferation and migration. Under co-culture conditions, both osteogenic differentiation and the formation of vessel-like angiogenic structures were suppressed by hypothermia, but the spatial organization of alkaline phosphatase-positive cell clusters, which tend to localize around microvascular lumens, was not altered. These data suggest that hypothermia attenuates heterotypic intercellular crosstalk which robustly depends on osteoblasts to inhibit both osteogenesis and angiogenesis in the co-culture system. Taken together, this approach will provide new insights into the relationship between osteoblasts and endothelial cells in tissue engineering.
Assembly of a Benthic Microbial Community in a Eutrophic Bay with a Long History of Oyster Culturing
Authors: Song Xiao , Song Junting , Yan Qi , Zhou Jin , Cai Zhonghua
Published: September 2021

The introduction of oysters to a waterbody is an efficient method for decreasing levels of eutrophication. Oysters affect sedimental environments and benthic microbes via their roles in nutrient cycling. However, little is known about how long-term oyster culturing affects benthic microbial community assembly. In the present study, top and bottom sediments from an oyster-culture area and non-culture area, in a eutrophic bay with a long history of oyster culturing, were obtained for environmental parameter measurement and microbe identification. Deterministic and stochastic processes in microbial community assembly were assessed. In particular, keystone species identification through network analysis was combined with measured environmental parameters to determine the factors related to community assembly processes. Our results suggest that oyster culturing relates to greater variation in both biological and non-biological sediment profiles. In benthic communities, Proteobacteria and Chloroflexi were the most abundant phyla, and community compositions were significantly different between sample groups. We also found that community assembly was more affected by deterministic factors than stochastic ones, when oysters were present. Moisture, or water content, and pH were identified as affecting deterministic and stochastic processes, respectively, but only water content was a driver associated with oyster culturing. Additionally, although keystone species presented a similar pattern of composition to peripheral species, they responded to their environments differently. Furthermore, model selection, fitting keystone species to community assembly processes, indicates their role in shaping microbial communities.
Comparative Genomic Analysis Determines the Functional Genes Related to Bile Salt Resistance in Lactobacillus salivarius
Authors: Pan Qiqi , Shen Xudan , Yu Leilei , Tian Fengwei , Zhao Jianxin , Zhang Hao , Chen Wei , Zhai Qixiao
Published: September 2021

Lactobacillus salivarius has drawn attention because of its promising probiotic functions. Tolerance to the gastrointestinal tract condition is crucial for orally administrated probiotics to exert their functions. However, previous studies of L. salivarius have only focused on the bile salt resistance of particular strains, without uncovering the common molecular mechanisms of this species. Therefore, in this study, we expanded our research to 90 L. salivarius strains to explore their common functional genes for bile salt resistance. First, the survival rates of the 90 L. salivarius strains in 0.3% bile salt solutions were determined. Comparative genomics analysis was then performed to screen for the potential functional genes related to bile salt tolerance. Next, real-time polymerase chain reaction and gene knockout experiments were conducted to further verify the tolerance-related functional genes. The results indicated that the strain-dependent bile salt tolerance of L. salivarius was mainly associated with four peptidoglycan synthesis-related genes, seven phosphotransferase system-related genes, and one chaperone-encoding gene involved in the stress response. Among them, the GATase1-encoding gene showed the most significant association with bile salt tolerance. In addition, four genes related to DNA damage repair and substance transport were redundant in the strains with high bile salt tolerance. Besides, cluster analysis showed that bile salt hydrolases did not contribute to the bile salt tolerance of L. salivarius. In this study, we determined the global regulatory genes, including LSL_1568, LSL_1716 and LSL_1709, for bile salt tolerance in L. salivarius and provided a potential method for the rapid screening of bile salt-tolerant L. salivarius strains, based on PCR amplification of functional genes.
The Characterization of a Novel Phage, pPa_SNUABM_DT01, Infecting Pseudomonas aeruginosa
Authors: Kwon Jun , Kim Sang Wha , Kim Sang Guen , Kang Jeong Woo , Jung Won Joon , Lee Sung Bin , Lee Young Min , Giri Sib Sankar , Chi Cheng , Park Se Chang
Published: September 2021

The bacterial genus Pseudomonas is a common causative agent of infections in veterinary medicine. In this study, we focused on Pseudomonas aeruginosa canine otitis externa isolates. Due to prolonged antibiotic treatment of otitis externa, antibiotic resistance is common and has become a major complication. Many alternatives to antibiotics have been studied, with bacteriophages emerging as the most promising alternatives. Here, we isolated and characterized a novel phage, pPa_SNUABM_DT01, by investigating its morphology, growth, lysis kinetics, and genomic characteristics. Phages have a vigorous capacity to eliminate bacterial cells through bacterial lysis. This capacity is dependent on the multiplicity of infection (MOI), but even at low MOIs, the phage successfully inhibited bacterial regrowth. The phage genome was 265,520 bp in size and comprised 312 putative open reading frames (ORFs). Comparative genome analysis demonstrated that the phage is a novel species in Myoviridae. The nucleotide similarity was moderately high compared with the Pseudomonas virus, Noxifer. However, a phylogenetic analysis and a dot plot indicated that pPa_SNUABM_DT01 is not closely related to the Phikzvirus or Noxifervirus genus but, instead, belongs to a novel one. The genome comparisons also indicate that the phage, pPa_SNUABM_DT01, could be a novel genus.
Editorial for Special Issue “Agates: Types, Mineralogy, Deposits, Host Rocks, Ages and Genesis”
Authors: Palyanova Galina
Published: September 2021

Agates are famous, beautiful, and fascinating stones found all around the world [...]
The Use of Limestones Built of Carbonate Phases with Increased Mg Content in Processes of Flue Gas Desulfurization
Authors: Stanienda-Pilecki Katarzyna Jadwiga
Published: September 2021

The purpose of this article was to present the theory of the possibility of using limestone sorbents containing carbonate minerals with increased Mg content in processes of flue gas desulfurization in power stations. Data obtained as a result of the conducted research show that the analyzed Triassic (Muschelkalk) limestones of the south-west part of Poland are built of the following carbonate minerals: low magnesium calcite, high magnesium calcite, dolomite and huntite. These carbonate minerals are characterized by various magnesium contents. The increased content of magnesium in the sorbent has a positive effect on the technological process of flue gas desulfurization using the dry method, especially in the case of desulfurization with the application of Fluidized Bed Reactors. The positive influence of magnesium’s presence in sorbent in the desulfurization process is connected with the decarbonization of carbonate phases with magnesium at temperatures similar to the dolomite decarbonization temperatures, which are, thus, lower than typical for low magnesium calcite. The process would be easier because the structure of a solid solution containing magnesium ions, with a smaller ion radius to calcium ions, is unstable and the decomposition of these phases runs easier and faster. Therefore, the desulfurization process starts earlier and is more effective.