bluuebunny/crossref_metadata_2025 huggingface dataset:
Case Report: Complete atrioventricular block in an elderly patient with acute pulmonary embolism
Authors: Kim Moojun , Seo Chang-ok , Kim Hangyul , Kim Hye Ree , Kim Kyehwan , Kang Min Gyu , Park Jeong Rang
Published: February 2024

Introduction

Multiple abnormal electrocardiographic findings have been documented in patients experiencing acute pulmonary embolism. Although sinus tachycardia is the most commonly encountered rhythmic disturbance, subsequent reports have highlighted other findings. These include right bundle branch block, right axis deviation, nonspecific ST segment/T wave changes, and T wave inversion in the right precordial leads. To date, only a limited number of cases involving a complete atrioventricular block have been reported in acute pulmonary embolism.

Case presentation

Here, we present the case of a 91-year-old woman with acute pulmonary embolism, whose initial electrocardiogram showed a complete atrioventricular block. She presented with presyncope and an initial blood pressure of 77/63 mmHg. Echocardiography confirmed signs of right ventricular dysfunction. Catheter-directed thrombolysis and a temporary pacemaker insertion were carried out sequentially. The following day, electrocardiography showed sinus rhythm with a left bundle branch block.

Discussion

The presence of a complete atrioventricular block in patients with acute pulmonary embolism serves as a clinical marker of high-risk status.
Building back better? The role of education sector responses in strengthening or eroding societal resilience during the COVID-19 pandemic
Authors: Shah Ritesh , Flemming Jennifer , Chinnery Julie , Heaner Gwen
Published: February 2024

COVID-19 laid bare many failings and shortcomings of state systems, institutions and structures which aim to protect citizens against harm. As observed from the outcomes of the pandemic in many parts of the Global South and Global North, the resilience of health, social protection, governance, economic, education, and social welfare systems has been significantly eroded or challenged, leaving large segments of the population open to heightened risk and vulnerability. This has been noted to have significantly eroded social capital, namely the trust, communication, and dialogue between citizens in the state as well as citizens with each other. Based on comparative research carried out on the return to schooling process in five countries, Lebanon, Nigeria, Colombia, Georgia, and Zambia, the paper highlights the important role and function that educational governance, decision-making, and control throughout the pandemic has played a key role in either maintaining or erode social capital, and ultimately the resilience of the entire education system. Much of this was based on how well state officials and local education officials both considered and communicated their actions to educators, students and their families. The importance of education as a site where both bridging and linking social capital is both strengthened, but also capitalized on is also discussed. Specifically, the paper highlights, using the example of COVID-19 education responses, how a state that is responsive and accountable to its citizenry in a time of crisis, stands the greatest chance of ensuring such periods do not lead to an erosion of social capital.
An improved multi-attribute group decision-making method for selecting the green supplier of community elderly healthcare service
Authors: Wang Lei , Zong Cuili , Shao Lianghai , Liu Zhi
Published: February 2024

With rapid social and economic development, the process of population aging has increased the demand for community elderly healthcare service (CEHS) in China. However, the traditional government-oriented service supply cannot meet the various needs of CEHS, and it is critical to select a suitable supplier of CEHS to provide high-quality green services in the community. Therefore, this study focuses on the issue of green supplier selection of CEHS, explores an improved transformation method for processing multi-type data, and proposes an integrated method of multi-attribute group decision-making (MAGDM) which innovatively applies the degree of overall deviation measure (ODM) to determine expert weight. Finally, the effectiveness and accuracy of the new method are verified by experimental analysis. The results show that H2 is the top choice in the green supplier selection of CEHS, followed by H1, H4, H8, H5, H6, H3, H7, H12, H11, H9, and H10. In addition, the authors apply the traditional ED method to calculate expert weights and compare the results of ODM and ED. It is a fact that the improved ODM method should be more efficient and accurate than the traditional ED method.
An environmental flow to an ephemeral creek increases the input of carbon and nutrients to a downstream receiving river
Authors: Liu Xiaoying , Watts Robyn J. , Dyer James
Published: February 2024

Although intermittent and ephemeral rivers lack surface flow for part of the year, they provide vital refuges for biota in otherwise dry semi-arid and arid landscapes. The hydrology of many such rivers has been altered due to river regulation and climate change. Environmental flows can be delivered to address the negative impacts of regulated flows, however there is limited knowledge of how dry ephemeral ecosystems respond following environmental flows. This study examined changes in water quality of the ephemeral Thule Creek in the southern Murray-Darling Basin, Australia, following delivery of environmental water from an irrigation canal. We also examined how the environmental flow influenced water quality of Wakool River that receives inflows from Thule Creek. Six sites in Thule Creek, three in Wakool River, and one in Yarraman irrigation channel (source water) were monitored for dissolved organic carbon (DOC), nutrients and dissolved oxygen (DO) once per week over 15 weeks from October 2019 to January 2020. The environmental flow resulted in high DOC concentrations (4.4–76 mg/L). Although low DO levels at sites in Thule Creek were recorded on some dates below the threshold for fish stress (< 4 mg/L) there were no fish kills observed during the environmental flow. The carbon-rich and nutrient-rich water (DOC >10 mg/L, total phosphorus up to 94 μg/L, total nitrogen up to 1,125 μg/L) was detected in the Wakool River downstream of Thule Creek confluence compared to the Wakool River upstream of Thule Creek confluence (DOC 6.6 mg/L, total phosphorus up to 64 μg/L, total nitrogen up to 805 μg/L) during the period when the environmental flow in Thule Creek was connected with the Wakool River. This research provides an example of how irrigation canal networks can be used to deliver environmental water to an ephemeral river to maintain refuges and contribute to the productivity of a receiving river further downstream. Careful management of the timing, volume and duration of environmental flows in arid or semi-arid landscapes is needed to avoid the development of poor water quality during, or following, the delivery of environmental water.
Comparative analysis of jujube and sour jujube gave insight into their difference in genetic diversity and suitable habitat
Authors: Shao Lingzhi , Qiao Ping , Wang Jingyi , Peng Yanfang , Wang Yiheng , Dong Wenpan , Li Jie
Published: February 2024

Jujube (Ziziphus jujuba var. jujuba Mill.) and sour jujube (Z. jujuba var. spinosa (Bunge) Hu ex H.F.Chow.) are economically, nutritionally, and ecologically significant members of the Rhamnaceae family. Despite their importance, insufficient research on their genetics and habitats has impeded effective conservation and utilization. To address this knowledge gap, we conducted plastome sequencing, integrated distribution data from China, and assessed genetic diversity and suitable habitat. The plastomes of both species exhibited high conservation and low genetic diversity. A new-found 23 bp species-specific Indel in the petL-petG enabled us to develop a rapid Indel-based identification marker for species discrimination. Phylogenetic analysis and dating illuminated their genetic relationship, showing speciation occurred 6.9 million years ago, in a period of dramatic global temperature fluctuations. Substantial variations in suitable climatic conditions were observed, with the mean temperature of the coldest quarter as the primary factor influencing distributions (−3.16°C–12.73°C for jujube and −5.79°C to 4.11°C for sour jujube, suitability exceeding 0.6). Consequently, distinct conservation strategies are warranted due to differences in suitable habitats, with jujube having a broader distribution and sour jujube concentrated in Northern China. In conclusion, disparate habitats and climatic factors necessitate tailored conservation approaches. Comparing genetic diversity and developing rapid species-specific primers will further enhance the sustainable utilization of these valuable species.
Lexical recognition processes in L2-dominant bilingualism
Authors: Bruggeman Laurence , Cutler Anne
Published: February 2024

To comprehend speech, listeners must resolve competition between potential candidate words. In second-language (L2) listening such competition may be inflated by spurious activation; the onsets of “reggae” and “legacy” may both activate “leg” for Japanese listeners, or the rhymes of “adapt” and “adept” may activate “apt” for Dutch listeners, while only one in each pair triggers competition for L1 listeners. Using eyetracking with L2-dominant bilingual emigrants, we directly compared within-language L1 and L2 lexical activation and competition in the same individuals. For these listeners, activation patterns did not differ across languages. Unexpectedly, however, we observed onset competition in both languages but rhyme competition in the L2 only (although the same stimuli elicited rhyme competition for control listeners in both languages). This suggests that L1 rhyme competition may disappear after long-time immersion in an L2 environment.
Towards environmental sustainability: further evidences from decarbonization projects in Kenya’s Blue Economy
Authors: Rasowo Joseph Ouma , Nyonje Betty , Olendi Robert , Orina Paul , Odongo Salome
Published: February 2024

Kenya is committed to the global efforts on climate change mitigation and adaptation as seen through investments in various sustainable green and blue economy projects. In this review paper, we present the current status of what has been done, particularly on the blue carbon offset initiatives undertaken in the mangrove and seaweed ecosystems as well as the decarbonization activities at the port of Mombasa and which should form reference information for local, regional, bilateral/multilateral partners, scientists and other climate change stakeholders. The blue carbon offset projects involve mangrove conservation, reforestation and carbon credit sale as well as seaweed farming. The initiatives have several unique features amongst which are the community-led income generation systems that simultaneously act as an inducement for ecosystem preservation, co-management and benefits sharing which are recipes for economic, socio-cultural, and environmental sustainability. A notable project impact is the conferment of economic power to the locals, particularly the women and the youth The model used embraces a collaborative approach involving multisectoral engagements of both the government, multilateral organizations, NGOs, and local communities. This integrated top-down (government) and bottom-up (local community) method deliberately targets the strengthening of economic development while ensuring sustainability.
Harnessing the joint effect of approach bridges in arch bridge construction: an analytical study on thrust stiffness and elevation error mitigation
Authors: Wang Shaorui , Feng Ji , Yang Ligui , Cai Guoqing , Jiang Daimin
Published: February 2024

Achieving full equilibrium for the horizontal component force of the backstay in cable-stayed arch bridges is challenging, and the stiffness of the buckle tower has a notable influence on the overall shape of the main arch structure. Increased stiffness in the buckle tower leads to reduced construction complexity. Therefore, this study proposed a method of enhancing the longitudinal thrust stiffness of the buckle tower using the joint effect of approach bridges. A sensitivity analysis was conducted on the approach bridge–composite buckle tower structure to determine the optimal combination method, resulting in the formulation of an analytical expression for the thrust stiffness of this structure. In this study, numerical analysis was performed to explore the composition mechanism of the thrust stiffness influenced by the pier–girder connection, and we discussed the applicability of the joint effect of approach bridges during the cantilever assembly process of arch ribs. The following conclusions were obtained: 1) prior to installing the main girder of the approach bridge, when the steel buckle tower and the junction pier have already been secured, the most effective approach is to form a “T” rigid structure by firmly connecting the main girder of the approach bridge with the composite buckle tower. This configuration provides self-weight deflection and pier–girder rotation restriction effects. 2) The study presents analytical formulas for the completely rigid pier–girder connection of the approach bridge–composite buckle tower structure, partially rigid pier–girder connection, and pre-deviation. Combined with the calculation program, this can guide structural design. 3) When a large downward elevation error of the arch ribs occurs in the middle and later stages, the cable force needed to install new arch segments becomes overly large. Therefore, the joint effect of approach bridges can be utilized to substitute for a portion of the cable force, effectively reducing potential elevation errors that might arise in subsequent arch ribs in the absence of this joint effect.
Biological treatment of pesticide-containing wastewater from coffee crops: selection and optimization of a biomixture and biobed design
Authors: Oviedo-Matamoros Fernando , Pérez-Villanueva Marta E. , Masís-Mora Mario , Aguilar-Álvarez Rónald , Ramírez-Morales Didier , Méndez-Rivera Michael , Rodríguez-Rodríguez Carlos E.
Published: February 2024

The biopurification systems (BPS) or biobeds are employed for the treatment of pesticide-containing wastewater of agricultural origin. The use of these devices for pesticide removal requires the proper optimization of the composition of biomixtures (BPS active matrix) according to the target pesticides applied on a specific crop and the available materials used in their elaboration. This work aims to design a biomixture for the simultaneous treatment of several pesticides applied in coffee crops, according to local practices in Costa Rica. Three biomixtures containing either coffee husk, coconut fiber or rice husk (as the lignocellulosic substrate) were applied for the removal of 12 pesticides. The profiles of pesticide elimination and the mineralization of radiolabeled chlorpyrifos (14C-chlorpyrifos) revealed that the best performance was achieved with the coconut fiber biomixture, even though similar detoxification patterns were determined in every biomixture (according to immobilization in Daphnia magna and germination tests in Lactuca sativa). The optimization of this biomixture’s composition by means of a central composite design permitted the definition of two optimal compositions (compost:soil:coconut fiber, % v/v) that maximized pesticide removal: i. 29:7.3:63.7 and ii. 11:7.3:81.7. The validation of these optimized compositions also included the use of an alternative soil from another coffee farm and resulted in overall DT50 values of 7.8–9.0 d for the pesticide mixture. Considering the removal kinetics in the optimized biomixture, a 1 m3 BPS prototype was dimensioned to be eventually used in local coffee farms. This work provides relevant information for the design and implementation of BPS at on-farm conditions for the treatment of pesticide-containing wastewater of a major crop.
Effect of TraN key residues involved in DNA binding on pIP501 transfer rates in Enterococcus faecalis
Authors: Michaelis Claudia , Berger Tamara M. I. , Kuhlmann Kirill , Ghulam Rangina , Petrowitsch Lukas , Besora Vecino Maria , Gesslbauer Bernd , Pavkov-Keller Tea , Keller Walter , Grohmann Elisabeth
Published: February 2024

Conjugation is a major mechanism that facilitates the exchange of antibiotic resistance genes among bacteria. The broad-host-range Inc18 plasmid pIP501 harbors 15 genes that encode for a type IV secretion system (T4SS). It is a membrane-spanning multiprotein complex formed between conjugating donor and recipient cells. The penultimate gene of the pIP501 operon encodes for the cytosolic monomeric protein TraN. This acts as a transcriptional regulator by binding upstream of the operon promotor, partially overlapping with the origin of transfer. Additionally, TraN regulates traN and traO expression by binding upstream of the PtraNO promoter. This study investigates the impact of nine TraN amino acids involved in binding to pIP501 DNA through site-directed mutagenesis by exchanging one to three residues by alanine. For three traN variants, complementation of the pIP501∆traN knockout resulted in an increase of the transfer rate by more than 1.5 orders of magnitude compared to complementation of the mutant with native traN. Microscale thermophoresis (MST) was used to assess the binding affinities of three TraN double-substituted variants and one triple-substituted variant to its cognate pIP501 double-stranded DNA. The MST data strongly correlated with the transfer rates obtained by biparental mating assays in Enterococcus faecalis. The TraN variants TraN_R23A-N24A-Q28A, TraN_H82A-R86A, and TraN_G100A-K101A not only exhibited significantly lower DNA binding affinities but also, upon complementation of the pIP501∆traN knockout, resulted in the highest pIP501 transfer rates. This confirms the important role of the TraN residues R23, N24, Q28, H82, R86, G100, and K101 in downregulating pIP501 transfer. Although TraN is not part of the mating pair formation complex, TraE, TraF, TraH, TraJ, TraK, and TraM were coeluted with TraN in a pull-down. Moreover, TraN homologs are present not only in Inc18 plasmids but also in RepA_N and Rep_3 family plasmids, which are frequently found in enterococci, streptococci, and staphylococci. This points to a widespread role of this repressor in conjugative plasmid transfer among Firmicutes.
Brain reward function in people who use cannabis: a systematic review
Authors: Beyer Emillie , Poudel Govinda , Antonopoulos Stephanie , Thomson Hannah , Lorenzetti Valentina
Published: February 2024

Rationale

Cannabis is one of the most widely used psychoactive substances globally. Cannabis use can be associated with alterations of reward processing, including affective flattening, apathy, anhedonia, and lower sensitivity to natural rewards in conjunction with higher sensitivity to cannabis-related rewards. Such alterations have been posited to be driven by changes in underlying brain reward pathways, as per prominent neuroscientific theories of addiction. Functional neuroimaging (fMRI) studies have examined brain reward function in cannabis users via the monetary incentive delay (MID) fMRI task; however, this evidence is yet to be systematically synthesised.

Objectives

We aimed to systematically integrate the evidence on brain reward function in cannabis users examined by the MID fMRI task; and in relation to metrics of cannabis exposure (e.g., dosage, frequency) and other behavioural variables.

Method

We pre-registered the review in PROSPERO and reported it using PRISMA guidelines. Literature searches were conducted in PsycINFO, PubMed, Medline, CINAHL, and Scopus.

Results

Nine studies were included, comprising 534 people with mean ages 16-to-28 years, of which 255 were people who use cannabis daily or almost daily, and 279 were controls. The fMRI literature to date led to largely non-significant group differences. A few studies reported group differences in the ventral striatum while participants anticipated rewards and losses; and in the caudate while participants received neutral outcomes. A few studies examined correlations between brain function and withdrawal, dosage, and age of onset; and reported inconsistent findings.

Conclusions

There is emerging but inconsistent evidence of altered brain reward function in cannabis users examined with the MID fMRI task. Future fMRI studies are required to confirm if the brain reward system is altered in vulnerable cannabis users who experience a Cannabis Use Disorder, as postulated by prominent neuroscientific theories of addiction.
Reduced menthol sensitivity in a prodromal Parkinson’s disease model induced by intranasal rotenone treatment
Authors: Sato Hajime , Satoh Keitaro , Nozaki Kazunori , Yugawa Misato , Kato Takafumi , Toyoda Hiroki , Katagiri Ayano , Suda Naoto , Adachi Kazunori
Published: February 2024

Parkinson’s disease (PD) is a neurodegenerative disorder characterized by motor symptoms, and it is associated with several prodromal non-motor symptoms, including an impaired sense of smell, taste and touch. We previously reported that bitter taste impairments occur independently of olfactory impairments in an early-stage PD animal model using short-term intranasal rotenone-treated mice. Cool temperatures also affect bitter taste perception, but it remains unclear whether or not bitter taste impairments result from an altered sensitivity for intraoral cool stimuli. We examined disturbances in the intraoral menthol sensitivity, such as coolness at low concentrations of menthol, using a brief-access test. Once a day, one solution from the 7-concentration series of (-)-menthol (0–2.3 mM) or the bitter taste quinine-HCl (0.3 mM) was randomly presented 20 times for 10 s to water-deprived mice before and 1 week after rotenone treatment. The total number of licks within 20 times was significantly decreased with the presentation of 2.3 mM menthol and quinine-HCl, compared to distilled water in untreated mice, but not in rotenone-treated mice. The correlation between the licks for quinine-HCl and that for menthol was increased after rotenone treatment. In contrast, the 2-bottle choice test for 48 h clarified that menthol sensitivity was increased after rotenone treatment. Furthermore, a thermal place preference test revealed that seeking behavior toward a cold-floored room was increased in the rotenone-treated mice despite the unchanged plantar cutaneous cold sensitivity. These results suggest that taste impairments in this model mice are at least partly due to intraoral somatosensory impairments, accompanied by peripheral/central malfunction.
Development of a feline model for preclinical research of a new translabyrinthine auditory nerve implant
Authors: Thomas W. Mitchel , Zuniga Steven A. , Sondh Inderbir , Leber Moritz , Solzbacher Florian , Lenarz Thomas , Lim Hubert H. , Warren David J. , Rieth Loren , Adams Meredith E.
Published: February 2024

Cochlear implants are among the most successful neural prosthetic devices to date but exhibit poor frequency selectivity and the inability to consistently activate apical (low frequency) spiral ganglion neurons. These issues can limit hearing performance in many cochlear implant patients, especially for understanding speech in noisy environments and in perceiving or appreciating more complex inputs such as music and multiple talkers. For cochlear implants, electrical current must pass through the bony wall of the cochlea, leading to widespread activation of auditory nerve fibers. Cochlear implants also cannot be implanted in some individuals with an obstruction or severe malformations of the cochlea. Alternatively, intraneural stimulation delivered via an auditory nerve implant could provide direct contact with neural fibers and thus reduce unwanted current spread. More confined current during stimulation can increase selectivity of frequency fiber activation. Furthermore, devices such as the Utah Slanted Electrode Array can provide access to the full cross section of the auditory nerve, including low frequency fibers that are difficult to reach using a cochlear implant. However, further scientific and preclinical research of these Utah Slanted Electrode Array devices is limited by the lack of a chronic large animal model for the auditory nerve implant, especially one that leverages an appropriate surgical approach relevant for human translation. This paper presents a newly developed transbullar translabyrinthine surgical approach for implanting the auditory nerve implant into the cat auditory nerve. In our first of a series of studies, we demonstrate a surgical approach in non-recovery experiments that enables implantation of the auditory nerve implant into the auditory nerve, without damaging the device and enabling effective activation of the auditory nerve fibers, as measured by electrode impedances and electrically evoked auditory brainstem responses. These positive results motivate performing future chronic cat studies to assess the long-term stability and function of these auditory nerve implant devices, as well as development of novel stimulation strategies that can be translated to human patients.
Evolution of the diagnosis of malnutrition in adults: a primer for clinicians
Authors: Hegazi Refaat , Miller Anthony , Sauer Abby
Published: February 2024

During the last two decades, the definition, diagnosis, and management of malnutrition have significantly evolved. Malnutrition is generally defined as deficiencies, excesses, or imbalances in a person’s intake of energy and/or nutrients. While malnutrition is associated with a significantly increased risk of morbidity, mortality, and healthcare cost, it is often underdiagnosed both in healthcare and community settings. One contributing factor is the lack of a consensus on its definition and appropriate diagnostic indicators. In the current article, we review the evolution of frameworks for the diagnosis of malnutrition. Recently published consensuses by prominent clinical nutrition societies have established a trajectory for the uniform global diagnosis of malnutrition. Limiting the use of body mass index (BMI) as a diagnostic criterion while emphasizing the use of muscle mass enables a more consistent and accurate diagnosis of malnutrition in the clinical setting. Guidance for the unified methodology and terminology for diagnosing malnutrition, such as the one proposed in the current article will enable policy makers to systematically address the two faces of malnutrition, starvation- and disease-related malnutrition applicable to both pediatric and adult populations. Policies and programs that could address issues of food insecurity and scarcity as well as early diagnosis and management of disease-related malnutrition will empower better care of community nutrition.
Characterization, antioxidant and antitumor activities of phenolic compounds from Amomum villosum Lour.
Authors: Zhang Ming , Shuai Xi-xiang , Wei Zhi , Dai Tao-tao , Wei Chang-bin , Li Ya , He Jun-jun , Du Li-qing
Published: February 2024

Amomum villosum Lour. (A. villosum), known as Sharen in China, is widely used for culinary and medicinal purposes due to containing a diverse set of bioactive compounds. In this study, the optimum ethanol extraction process was optimized and the composition and biological activities (antioxidant and antitumor) of five different fractions (dichloromethane, petroleum ether, ethyl acetate, n-butanol and H2O) extracted from the ethanol extract of A. villosum were investigated. The results showed that the optimal extraction conditions were extraction temperature 80°C, extraction time 120 min, ethanol concentration 40% and solid–liquid ratio 1:25 g/mL. Moreover, 35 bioactive compounds were successfully identified by UPLC-ESI-QTOF-MS/MS from five factions for the first time, including 12 phenolic acids and derivatives, 2 organic acids, 12 flavonoids and derivatives, 2 oxylipins and 7 proanthocyanidins. Among them, ethyl acetate fraction (Fr-EtOAc) exhibited the highest content of total phenolic (374.01 mg GAE/g DW) and flavonoid (93.11 mg RE/g DW), where vanillic acid, catechin, epicatechin and protocatechuic acid were the predominant phenolic compounds that accounting for 81.65% of the quantified bioactive compounds. In addition, Fr-EtOAc demonstrated excellent total antioxidant activity (IC50 of DPPH and ABTS assays were 0.23, 0.08 mg/mL, respectively, and FRAP assay was 322.91 mg VCE/100 g DW) and antitumor activity (1,000 μg/mL, 79.04% inhibition rate). The results could provide guidance for the industrial production and application of A. villosum.
The interplay of food-related lifestyle and eating behavior in Italian women
Authors: Hamam Manal , D’Amico Mario , Spina Daniela , La Via Giovanni , Di Vita Giuseppe
Published: February 2024

Introduction

Women play a crucial role in food shopping and preparation, and their food choices have significant implications for their health and that of their families. This study aims to provide a perspective on women’s eating lifestyle, which has undergone significant changes.

Methods

A factor analysis was conducted to assess the degree of involvement in food choices and the types of food items consumed among a sample of 399 Italian women.

Results

Through cluster analysis, four segments were identified: hedonic food consumers, sustainable- and balanced-diet consumers, food experimenters, and no food fondness consumers. The results reveal a correlation between the degree of food involvement and the type of food consumed.

Discussion

Furthermore, the food lifestyle of the sample is partially dependent on age. Individuals aged 25–28 years show more hedonic food consumption behavior, while the older age group (44–64 years) falls into the sustainable and balanced diet consumer cluster (the largest cluster) and the cluster of those who do not express definable food choices (no food fondness).
Case report: Gastric carcinoma with SMARCA4 deficient: two cases report and literature review
Authors: Lin Zeyang , Li Qian , He Yujie , Guo Shujing , Ye Yuhan , Liu Zhengjin
Published: February 2024

SMARCA4-deficient gastric carcinoma has been reported sporadically since 2016. Only 29 patients have been reported; nevertheless, it is aggressive and highly malignant with poor outcomes. It has an immunohistochemical phenotype showing loss of SMARCA4 expression and can be accompanied by codeletion of other switch/sucrose non-fermentable chromatin-remodeling complex subunits. Microscopically, it displays high-grade undifferentiated histological morphology with rhabdoid cell differentiation. Rarely does the tumor contain a purely or partly adenocarcinoma component. Here, we report two cases to demonstrate these unusual morphologies analyzed using morphological and immunohistochemical techniques. In addition, there is a lack of research on the classification of these morphologies. Therefore, our report will aid the diagnosis and classification of SMARCA4-deficient gastric carcinoma.
Case report: Response to Savolitinib/EGFR-TKI combination in NSCLC patients harboring concurrent primary MET amplification/overexpression and EGFR mutation
Authors: Ren Xiaolin , Li Kejie , Zhang Yang , Zou Changlin , Su Meng
Published: February 2024

Lung cancer is the leading cause of cancer death, accounting for one-third of all cancer deaths worldwide. The MET (c-MET) gene, as one of the therapeutic target spots of NSCLC, has become increasingly more important. MET amplification/overexpression was divided into primary (intrinsic) and secondary (acquired). Studies indicated that the combination of Osimertinib and Savolitinib was safe and showed promising antitumor effect in NSCLC patients with secondary MET amplification after EGFR mutations. However, NSCLC patients with primary MET amplification/overexpression and EGFR mutations are rare in clinics, and the efficacy of dual-target therapy combined with EGFR-TKI and Savolitinib for them has not been studied yet. Here, we reported two NSCLC patients with primary MET amplification/overexpression and EGFR mutation, who benefited from T+S therapy (the dual-target therapy of EGFR-TKI plus Savolitinib) and achieved a progression-free survival (PFS) of approximately 5 months. The two cases indicated that T+S therapy has an acceptable safety profile and encouraging antitumor efficacy in NSCLC patients harboring concurrent primary MET amplification/overexpression and EGFR mutation. Meanwhile, the observation stresses the importance of genetic testing, and the MET gene needs to be detected at first diagnosis for the best choice of targeted therapies.
Predicting the status of lymphovascular space invasion using quantitative parameters from synthetic MRI in cervical squamous cell carcinoma without lymphatic metastasis
Authors: Guo Limei , Zhang Runmei , Xu Yi , Wu Wenqi , Zheng Qian , Li Jianting , Wang Jun , Niu Jinliang
Published: February 2024

Purpose

To investigate the value of quantitative longitudinal relaxation time (T1), transverse relaxation time (T2), and proton density (PD) maps derived from synthetic magnetic resonance imaging (MRI) for evaluating the status of lymphovascular space invasion (LVSI) in cervical squamous cell carcinoma (CSCC) without lymph node metastasis (LNM).

Material and methods

Patients with suspected cervical cancer who visited our hospital from May 2020 to March 2023 were collected. All patients underwent preoperative MRI, including routine sequences and synthetic MRI. Patients with pathologically confirmed CSCC without lymphatic metastasis were included in this study. The subjects were divided into negative- and positive-LVSI groups based on the status of LVSI. Quantitative parameters of T1, T2, and PD values derived from synthetic MRI were compared between the two groups using independent samples t-test. Receiver operating characteristic curves were used to determine the diagnostic efficacy of the parameters.

Results

59 patients were enrolled in this study and were classified as positive (n = 32) and negative LVSI groups (n = 27). T1 and T2 values showed significant differences in differentiating negative-LVSI from positive-LVSI CSCC (1307.39 ± 122.02 vs. 1193.03 ± 107.86, P<0.0001; 88.42 ± 7.24 vs. 80.99 ± 5.50, P<0.0001, respectively). The area under the curve (AUC) for T1, T2 values and a combination of T1 and T2 values were 0.756, 0.799, 0.834 respectively, and there is no statistically significant difference in the diagnostic efficacy between individual and combined diagnosis of each parameter.

Conclusions

Quantitative parameters derived from synthetic MRI can be used to evaluate the LVSI status in patients with CSCC without LNM.
Should we “eliminate” PDA shunt in preterm infants? A narrative review
Authors: Surak Aimann , Sidhu Amneet , Ting Joseph Y.
Published: February 2024

The patent ductus arteriosus frequently poses a significant morbidity in preterm infants, subjecting their immature pulmonary vascular bed to substantial volume overload. This, in turn, results in concurrent hypoperfusion to post-ductal organs, and subsequently alters cerebral blood flow. In addition, treatment has not demonstrated definitive improvements in patient outcomes. Currently, the optimal approach remains a subject of considerable debate with ongoing research controversy regarding the best approach. This article provides a comprehensive review of existing literature.
Case Report: The acute appendicitis and incarcerated umbilical hernia: a rare association
Authors: Corbi Letizia , Frediani Simone , Aloi Ivan Pietro , Bertocchini Arianna , Accinni Antonella , Pardi Valerio , Inserra Alessandro
Published: February 2024

Introduction

One of the most prevalent congenital wall abnormalities in children, umbilical hernias are often linked to premature or small-for-gestational-age babies. In cases of intestinal malrotation or if the cecum is very movable, generalized peritonitis may facilitate the imprisonment of these hernias.

Case report

We described a case of a 4-month-old baby who had a prior reducible umbilical hernia with a history of fever, vomiting, poor appetite, and constipation for around 48 h. The patient experienced significant intestinal bloating, vomiting, irreducibility of the umbilical hernia, skin pigmentation, and erythema at the umbilical site within 2 days after hospitalization. When there was no free abdominal air, a direct abdominal x-ray revealed evidence of hydro-gas stasis and various hydro-aerial levels that were pertinent to the ileum. In order to reduce the hernia, the patient had an emergency surgical treatment where the hernia sac was isolated and released from the ileal loop, which was securely attached to a fibrin plate. When the herniary sac was opened, a gangrenous and perforated appendix was found inside. On the seventh postoperative day, the patient was released from the hospital after an uncomplicated postoperative stay.

Conclusion

Our patient's clinical presentation is similar to that of only one other case report involving a 25-day-old male patient. Our case presented with a variant of the clinical symptoms of the previously described umbilical hernia, which became unfixable and strangulated as a result of appendix inflammation. The appendix was discovered inside the hernia sac during surgery.
Individual ingredients of NP-101 (Thymoquinone formula) inhibit SARS-CoV-2 pseudovirus infection
Authors: Maen Abdelrahim , Gok Yavuz Betul , Mohamed Yehia I. , Esmail Abdullah , Lu Jianming , Mohamed Amr , Azmi Asfar S. , Kaseb Mohamed , Kasseb Osama , Li Dan , Gocio Michelle , Kocak Mehmet , Selim Abdelhafez , Ma Qing , Kaseb Ahmed O.
Published: February 2024

Thymoquinone TQ, an active ingredient of Nigella Sativa, has been shown to inhibit COVID-19 symptoms in clinical trials. Thymoquinone Formulation (TQF or NP-101) is developed as a novel enteric-coated medication derivative from Nigella Sativa. TQF consists of TQ with a favorable concentration and fatty acids, including palmitic, oleic, and linoleic acids. In this study, we aimed to investigate the roles of individual ingredients of TQF on infection of SARS-CoV-2 variants in-vitro, by utilizing Murine Leukemia Virus (MLV) based pseudovirus particles. We demonstrated that NP-101, TQ, and other individual ingredients, including oleic, linoleic, and palmitic acids inhibited SARS-CoV-2 infection in the MLV-based pseudovirus model. A large, randomized phase 2 study of NP-101 is planned in outpatient COVID-19 patients.
Optimization of soybean physiochemical, agronomic, and genetic responses under varying regimes of day and night temperatures
Authors: Ding Chuanbo , Alghabari Fahad , Rauf Muhammad , Zhao Ting , Javed Muhammad Matloob , Alshamrani Rahma , Ghazy Abdel-Halim , Al-Doss Abdullah A. , Khalid Taimoor , Yang Seung Hwan , Shah Zahid Hussain
Published: February 2024

Soybean is an important oilseed crop worldwide; however, it has a high sensitivity to temperature variation, particularly at the vegetative stage to the pod-filling stage. Temperature change affects physiochemical and genetic traits regulating the soybean agronomic yield. In this regard, the current study aimed to comparatively evaluate the effects of varying regimes of day and night temperatures (T1 = 20°C/12°C, T2 = 25°C/17°C, T3 = 30°C/22°C, T4 = 35°C/27°C, and T5 = 40°C/32°C) on physiological (chlorophyll, photosynthesis, stomatal conductance, transpiration, and membrane damage) biochemical (proline and antioxidant enzymes), genetic (GmDNJ1, GmDREB1G;1, GmHSF-34, GmPYL21, GmPIF4b, GmPIP1;6, GmGBP1, GmHsp90A2, GmTIP2;6, and GmEF8), and agronomic traits (pods per plant, seeds per plant, pod weight per plant, and seed yield per plant) of soybean cultivars (Swat-84 and NARC-1). The experiment was performed in soil plant atmosphere research (SPAR) units using two factorial arrangements with cultivars as one factor and temperature treatments as another factor. A significant increase in physiological, biochemical, and agronomic traits with increased gene expression was observed in both soybean cultivars at T4 (35°C/27°C) as compared to below and above regimes of temperatures. Additionally, it was established by correlation, principal component analysis (PCA), and heatmap analysis that the nature of soybean cultivars and the type of temperature treatments have a significant impact on the paired association of agronomic and biochemical traits, which in turn affects agronomic productivity. Furthermore, at corresponding temperature regimes, the expression of the genes matched the expression of physiochemical traits. The current study has demonstrated through extensive physiochemical, genetic, and biochemical analyses that the ideal day and night temperature for soybeans is T4 (35°C/27°C), with a small variation having a significant impact on productivity from the vegetative stage to the grain-filling stage.
Spatio-temporal transcriptome and storage compound profiles of developing faba bean (Vicia faba) seed tissues
Authors: Ohm Hannah , Saripella Ganapathi Varma , Hofvander Per , Grimberg Åsa
Published: February 2024

Faba bean (Vicia faba) is a legume grown in diverse climate zones with a high potential for increased cultivation and use in food due to its nutritional seeds. In this study, we characterized seed tissue development in faba bean to identify key developmental processes; from embryo expansion at the expense of the endosperm to the maturing storage stages of the bean seed. A spatio-temporal transcriptome profiling analysis, combined with chemical nutrient analysis of protein, starch, and lipid, of endosperm and embryo tissues at different developmental stages, revealed gene expression patterns, transcriptional networks, and biochemical pathways in faba bean. We identified key players in the LAFL (LEC1, ABI3, FUS3, and LEC2) transcription factor network as well as their major repressors VAL1 and ASIL1. Our results showed that proteins accumulated not only in the embryo but also in the endosperm. Starch accumulated throughout seed development and oil content increased during seed development but at very low levels. The patterns of differentially expressed transcripts encoding proteins with functions in the corresponding metabolic pathways for the synthesis of these storage compounds, to a high extent, aligned with these findings. However, the early expression of transcripts encoding WRI1 combined with the late expression of oil body proteins indicated a not manifested high potential for lipid biosynthesis and oil storage. Altogether, this study contributes to increased knowledge regarding seed developmental processes applicable to future breeding methods and seed quality improvement for faba bean.
Histological, chemical and gene expression differences between western redcedar seedlings resistant and susceptible to cedar leaf blight
Authors: Aldana Juan A. , Moa Belaid , Mattsson Jim , Russell John H. , Hawkins Barbara J.
Published: February 2024

Introduction

Western redcedar (Thuja plicata) is an important species in the Cupressaceae both at economic and cultural levels in the Pacific Northwest of North America. In adult trees, the species produces one of the most weathering-resistant heartwoods among conifers, making it one of the preferred species for outdoor applications. However, young T. plicata plants are susceptible to infection with cedar leaf blight (Didymascella thujina), an important foliar pathogen that can be devastating in nurseries and small-spaced plantations. Despite that, variability in the resistance against D. thujina in T. plicata has been documented, and such variability can be used to breed T. plicata for resistance against the pathogen.

Objective

This investigation aimed to discern the phenotypic and gene expression differences between resistant and susceptible T. plicata seedlings to shed light on the potential constitutive resistance mechanisms against cedar leaf blight in western redcedar.

Methods

The study consisted of two parts. First, the histological differences between four resistant and four susceptible families that were never infected with the pathogen were investigated. And second, the differences between one resistant and one susceptible family that were infected and not infected with the pathogen were analyzed at the chemical (C, N, mineral nutrients, lignin, fiber, starch, and terpenes) and gene expression (RNA-Seq) levels.

Results

The histological part showed that T. plicata seedlings resistant to D. thujina had constitutively thicker cuticles and lower stomatal densities than susceptible plants. The chemical analyses revealed that, regardless of their infection status, resistant plants had higher foliar concentrations of sabinene and α-thujene, and higher levels of expression of transcripts that code for leucine-rich repeat receptor-like protein kinases and for bark storage proteins.

Conclusion

The data collected in this study shows that constitutive differences at the phenotypic (histological and chemical) and gene expression level exist between T. plicata seedlings susceptible and resistant to D. thujina. Such differences have potential use for marker-assisted selection and breeding for resistance against cedar leaf blight in western redcedar in the future.
Enhancing growth, vitality, and aromatic richness: unveiling the dual magic of silicon dioxide and titanium dioxide nanoparticles in Ocimum tenuiflorum L.
Authors: Bhat Urooj Hassan , Uddin Moin , Chishti Aman Sobia , Singh Sangram , Singh Sarika , Khan M. Masroor A. , Mukarram Mohammad
Published: February 2024

Ocimum tenuiflorum, commonly known as “Holy basil,” is renowned for its notable medicinal and aromatic attributes. Its unique fragrance attributes to specific volatile phytochemicals, primarily belonging to terpenoid and/or phenylpropanoid classes, found within their essential oils. The use of nanoparticles (NPs) in agriculture has attracted attention among plant researchers. However, the impact of NPs on the modulation of morpho-physiological aspects and essential oil production in medicinal plants has received limited attention. Consequently, the present study aimed to explore the effect of silicon dioxide (SiO2) and titanium dioxide (TiO2) nanoparticles at various concentrations (viz., DDW (control), Si50+Ti50, Si100+Ti50, Si100+Ti100, Si200+Ti100, Si100+Ti200 and Si200+Ti200 mg L-1) on growth, physiology and essential oil production of O. tenuiflorum at 120 days after planting (DAP). The results demonstrated that the combined application of Si and Ti (Si100+Ti100 mg L-1) exhibited the most favourable outcomes compared to the other combinational treatments. This optimal treatment significantly increased the vegetative growth parameters (root length (33.5%), shoot length (39.2%), fresh weight (62.7%) and dry weight (28.5%)), photosynthetic parameters, enzymatic activities (nitrate reductase and carbonic anhydrase), the overall area of PGTs (peltate glandular trichomes) and essential oil content (172.4%) and yield (323.1%), compared to the control plants. Furthermore, the GCMS analysis showed optimal treatment (Si100+Ti100) significantly improved the content (43.3%) and yield (151.3%) of eugenol, the primary active component of the essential oil. This study uncovers a remarkable and optimal combination of SiO2 and TiO2 nanoparticles that effectively enhances the growth, physiology, and essential oil production in Holy basil. These findings offer valuable insights into maximizing the potential benefits of its use in industrial applications.