bluuebunny/crossref_metadata_2025 huggingface dataset:
Patients’ experience on pain outcomes after hip arthroplasty: insights from an information tool based on registry data
Authors: Fabiano Gianluca , Cole Sophie , Barea Christophe , Cullati Stéphane , Agoritsas Thomas , Gutacker Nils , Silman Alan , Hannouche Didier , Lübbeke Anne , Pinedo-Villanueva Rafael
Published: April 2024

Background

Arthroplasty registries are rarely used to inform encounters between clinician and patient. This study is part of a larger one which aimed to develop an information tool allowing both to benefit from previous patients’ experience after total hip arthroplasty (THA). This study focuses on generating the information tool specifically for pain outcomes.

Methods

Data from the Geneva Arthroplasty Registry (GAR) about patients receiving a primary elective THA between 1996 and 2019 was used. Selected outcomes were identified from patient and surgeon surveys: pain walking, climbing stairs, night pain, pain interference, and pain medication. Clusters of patients with homogeneous outcomes at 1, 5, and 10 years postoperatively were generated based on selected predictors evaluated preoperatively using conditional inference trees (CITs).

Results

Data from 6,836 THAs were analysed and 14 CITs generated with 17 predictors found significant (p < 0.05). Baseline WOMAC pain score, SF-12 self-rated health (SRH), number of comorbidities, SF-12 mental component score, and body mass index (BMI) were the most common predictors. Outcome levels varied markedly by clusters whilst predictors changed at different time points for the same outcome. For example, 79% of patients with good to excellent SRH and less than moderate preoperative night pain reported absence of night pain at 1 year after THA; in contrast, for those with fair/poor SHR this figure was 50%. Also, clusters of patients with homogeneous levels of night pain at 1 year were generated based on SRH, Charnley, WOMAC night and pain scores, whilst those at 10 years were based on BMI alone.

Conclusions

The information tool generated under this study can provide prospective patients and clinicians with valuable and understandable information about the experiences of “patients like them” regarding their pain outcomes.
Role of Galectin-3 in intervertebral disc degeneration: an experimental study
Authors: Li Jianjiang , Han Nianrong , Liu Zhenqiang , Osman Akram , Xu Leilei , Song Jing , Xiao Yang , Hu Wei
Published: April 2024

Background

This study investigated the role of Galectin-3 in the degeneration of intervertebral disc cartilage.

Methods

The patients who underwent lumbar spine surgery due to degenerative disc disease were recruited and divided into Modic I, Modic II, and Modic III; groups. HE staining was used to detect the pathological changes in endplates. The changes of Galectin-3, MMP3, Aggrecan, CCL3, and Col II were detected by immunohistochemistry, RT-PCR, and Western blot. MTT and flow cytometry were used to detect cartilage endplate cell proliferation, cell cycle, and apoptosis.

Results

With the progression of degeneration (from Modic I to III), the chondrocytes and density of the cartilage endplate of the intervertebral disc decreased, and the collagen arrangement of the cartilage endplate of the intervertebral disc was broken and calcified. Meanwhile, the expressions of Aggrecan, Col II, Galectin-3, Aggrecan, and CCL3 gradually decreased. After treatment with Galectin-3 inhibitor GB1107, the proliferation of rat cartilage end plate cells was significantly reduced (P < 0.05). GB1107 (25 µmol/L) also significantly promoted the apoptosis of cartilage endplate cells (P < 0.05). Moreover, the percentage of cartilage endplate cells in the G1 phase was significantly higher, while that in the G2 and S phases was significantly lower (P < 0.05). Additionally, the mRNA and protein expression levels of MMP3, CCL3, and Aggrecan in rat cartilage end plate cells were lower than those in the control group.

Conclusions

Galectin-3 decreases with the progression of the cartilage endplate degeneration of the intervertebral disc. Galectin-3 may affect intervertebral disc degeneration by regulating the degradation of the extracellular matrix.
Views of admitted palliative care patients and their clinicians on corneal donation discussions: a qualitative content analysis of semi-structured interviews
Authors: Patel Chirag , Nicmanis Mitchell , Chur-Hansen Anna , Crawford Gregory B.
Published: April 2024

Background

There is a severe shortage of corneas for donation, globally, for transplantation and research purposes. One group of individuals who could potentially be donors are those who die within the inpatient palliative care unit. The aim of the study was to understand clinician and patient perceptions of corneal donations and discussion of donation in palliative care units.

Methods

A qualitative design was utilised with data collected through semi-structured interviews and analysed using qualitative content analysis. A total of 46 interviews were undertaken involving inpatient palliative care unit patients (19) and clinicians (27) in three major inpatient palliative care units in South Australia.

Results

Very few patient participants reported being asked about corneal donations during their time in palliative care. Most inpatient palliative care unit clinicians did not raise the topic as they felt other areas of care took precedence. Inpatient palliative care unit patients thought if inpatient palliative care unit clinicians did not raise the topic, then it was not important. There were some differences between patient and clinician views, such as preference about who raises the possibility of donation and when the discussion might occur.

Conclusions

Findings suggest that patients are receptive to discussing corneal donations, but clinicians are not initiating these. This is a missed opportunity for donors and potential recipients. We recommend that clinicians routinely discuss eye donation as part of palliative care.
A preliminary, quantitative study on the use of traditional and complementary medicine by cancer patients seen at the Senkatana oncology clinic, Maseru, Lesotho
Authors: Sooro Mopa A. , Thoahlane Thabo S. , Ramathebane Maseabata V. , Mputsoe Kabelo A.
Published: April 2024

Background

The use of traditional and complementary medicine (TCM) by cancer patients remains common in several countries especially in the Sub-Saharan Africa. However, the reasons for use are complex and change with time and geographic location, they may vary from therapy to therapy, and they are different from one individual to another. The use of TCM has been associated with active coping behaviour and a way through which patients take control of their own health. However, cancer patients do not disclose their use of TCM to the attending healthcare professionals and therefore the effects of these medicines on the patients may not be ascertained.

Aim

To investigate the use of traditional and complementary medicines among patients diagnosed with cancer.

Methods

A cross-sectional, quantitative study was conducted at Senkatana Oncology clinic in May to June 2023. Cancer patients underwent standardized, quantitative interviews using structured questionnaires about their use of TCM. Descriptive statistics were used to analyse the data. Logistic regression analysis was also used to identify factors associated with satisfaction with the performance of TCM.

Results

All interviewed patients (n = 50, 100%) reported to be using TCM. Patients consisted of 24 females (48%) and 26 males (52%) in the age range 14 to 82 years old. The majority of the study population was in the age group 35–44 years old. The most prevalent cancer among participating males was prostate cancer and among females was cervical cancer. Biological products use was the most prominent with the highest average percentage usage (14.7%). The majority of patients (66%, n = 33) indicated that they just wanted to try everything that could help. Patients (n = 47, 94%) further reported that they had been using complementary medicine during the same period as they were using conventional treatment so that both may work to help each other. Neither gender nor age predicted satisfaction with the performance of traditional and complementary medicine.

Conclusions

It is concluded that all interviewed cancer patients use TCM. Patients indicated that one of the reasons for using TCM was that they wanted to try everything that could help in their cancer care. Patients further reported that they did not inform their oncologist of their concurrent use of TCM because they had been advised not to use other medicines besides what they are given at the clinic.
Knowledge, attitudes, and practice toward postoperative cognitive dysfunction among anesthesiologists in China: a cross-sectional study
Authors: Hu Li , Kang Shuai , Peng Qiaoyi , An Erdan , Lu Jian , Yang Hao , Zhou Hongmei , Zhang Bin
Published: April 2024

Background

To investigate the knowledge, attitudes, and practice (KAP) toward postoperative cognitive dysfunction (POCD) among anesthesiologists in China.

Methods

This cross-sectional study was conducted nationwide among Chinese anesthesiologists between December 2022 and January 2023. The demographic information and KAP scores of the respondents were collected using a web-based questionnaire. The mean KAP dimension scores ≥ 60% were considered good.

Results

This study enrolled 1032 anesthesiologists (51.2% male). The mean total scores of knowledge, positive attitude, and positive practice were 9.3 ± 1.2 (max 12), 34.8 ± 3.3 (max 40), and 30.6 ± 6.7 (max 40), respectively. The knowledge items with correctness scores < 60% were “the anesthetic drugs that tend to cause POCD” (23.3%) and “Treatment of POCD” (40.3%). Multivariable analysis showed that ≥ 40 years old, master’s degree or above, intermediate professional title (i.e., attending physician), senior professional title (i.e., chief physician), and working in tertiary hospitals were independently associated with adequate knowledge. Multivariable analysis showed that the attitude scores, middle professional title, and ≥ 16 years of experience were independently associated with good practice.

Conclusions

These results suggest that Chinese anesthesiologists have good knowledge, favorable attitudes, and good practice toward POCD. Still, some points remain to be improved (e.g., the drugs causing POCD and managing POCD) and should be emphasized in training and continuing education.

Trial registration

ChiCTR2200066749.
Antibacterial effects of chitosan-based hydrogels containing Trachyspermum ammi essential oil on pathogens isolated from dogs with otitis externa
Authors: Niaraki Niloofar Jelokhani , Jamshidi Shahram , Fasaei Bahar Nayeri , Joghataei Seyed Mehdi
Published: April 2024

Background

Growing antibiotic resistance has made treating otitis externa (OE) increasingly challenging. On the other hand, local antimicrobial treatments, especially those that combine essential oils (EOs) with nanoparticles, tend to be preferred over systemic ones. It was investigated whether Ajwain (Trachyspermum ammi) EO, combined with chitosan nanoparticles modified by cholesterol, could inhibit the growth of bacterial pathogens isolated from OE cases in dogs. In total, 57 dogs with clinical signs of OE were examined and bacteriologically tested. Hydrogels of Chitosan were synthesized by self-assembly and investigated. EO was extracted (Clevenger machine), and its ingredients were checked (GC-MS analysis) and encapsulated in chitosan-cholesterol nanoparticles. Disc-diffusion and broth Micro-dilution (MIC and MBC) examined its antimicrobial and therapeutic properties.

Results

Staphylococcus pseudintermedius (49.3%) was the most common bacteria isolated from OE cases, followed by Pseudomonas aeruginosa (14.7%), Escherichia coli (13.3%), Streptococcus canis (9.3%), Corynebacterium auriscanis (6.7%), Klebsiella pneumoniae (2.7%), Proteus mirabilis (2.7%), and Bacillus cereus (1.3%). The investigation into the antimicrobial properties of Ajwain EO encapsulated in chitosan nanoparticles revealed that it exhibited a more pronounced antimicrobial effect against the pathogens responsible for OE.

Conclusions

Using chitosan nanoparticles encapsulated with EO presents an effective treatment approach for dogs with OE that conventional antimicrobial treatments have not cured. This approach not only enhances antibacterial effects but also reduces the required dosage of antimicrobials, potentially preventing the emergence of antimicrobial resistance.
Significant but partial lipoprotein lipase functional loss caused by a novel occurrence of rare LPL biallelic variants
Authors: Hu Yuepeng , Chen Jian-Min , Zuo Han , Pu Na , Zhang Guofu , Duan Yichen , Li Gang , Tong Zhihui , Li Weiqin , Li Baiqiang , Yang Qi
Published: April 2024

Background

Lipoprotein lipase (LPL) plays a crucial role in triglyceride hydrolysis. Rare biallelic variants in the LPL gene leading to complete or near-complete loss of function cause autosomal recessive familial chylomicronemia syndrome. However, rare biallelic LPL variants resulting in significant but partial loss of function are rarely documented. This study reports a novel occurrence of such rare biallelic LPL variants in a Chinese patient with hypertriglyceridemia-induced acute pancreatitis (HTG-AP) during pregnancy and provides an in-depth functional characterization.

Methods

The complete coding sequences and adjacent intronic regions of the LPL, APOC2, APOA5, LMF1, and GPIHBP1 genes were analyzed by Sanger sequencing. The aim was to identify rare variants, including nonsense, frameshift, missense, small in-frame deletions or insertions, and canonical splice site mutations. The functional impact of identified LPL missense variants on protein expression, secretion, and activity was assessed in HEK293T cells through single and co-transfection experiments, with and without heparin treatment.

Results

Two rare LPL missense variants were identified in the patient: the previously reported c.809G > A (p.Arg270His) and a novel c.331G > C (p.Val111Leu). Genetic testing confirmed these variants were inherited biallelically. Functional analysis showed that the p.Arg270His variant resulted in a near-complete loss of LPL function due to effects on protein synthesis/stability, secretion, and enzymatic activity. In contrast, the p.Val111Leu variant retained approximately 32.3% of wild-type activity, without impacting protein synthesis, stability, or secretion. Co-transfection experiments indicated a combined activity level of 20.7%, suggesting no dominant negative interaction between the variants. The patient’s post-heparin plasma LPL activity was about 35% of control levels.

Conclusions

This study presents a novel case of partial but significant loss-of-function biallelic LPL variants in a patient with HTG-AP during pregnancy. Our findings enhance the understanding of the nuanced relationship between LPL genotypes and clinical phenotypes, highlighting the importance of residual LPL function in disease manifestation and severity. Additionally, our study underscores the challenges in classifying partial loss-of-function variants in classical Mendelian disease genes according to the American College of Medical Genetics and Genomics (ACMG)’s variant classification guidelines.
MFGE8 in exosomes derived from mesenchymal stem cells prevents esophageal stricture after endoscopic submucosal dissection in pigs
Authors: Lai Huasheng , Yip Hon-Chi , Gong Yu , Chan Kai-Fung , Leung Kevin Kai-Chung , Chan Melissa Shannon , Xia Xianfeng , Chiu Philip Wai-Yan
Published: April 2024

Background

Endoscopic submucosal dissection (ESD) is the current standard treatment for early-stage esophageal neoplasms. However, the postoperative esophageal stricture after extensive mucosal dissection remains a severe challenge with limited effective treatments available. In this study, we introduced a chitosan/gelatin (ChGel) sponge encapsulating the adipose mesenchymal stem cells (ADMSCs)-derived exosomes (ChGelMSC−Exo) for the prevention of esophageal stenosis after ESD in a porcine model.

Results

Pigs were randomly assigned into (1) ChGelMSC−Exo treatment group, (2) ChGelPBS group, and (3) the controls. Exosome treatments were applied immediately on the day after ESD as well as on day 7. Exosome components crucial for wound healing were investigated by liquid chromatography-tandem mass spectrometry (LC–MS/MS) and small RNA sequencing. ChGelMSC−Exo treatment significantly reduced mucosal contraction on day 21, with less fiber accumulation and inflammatory infiltration, and enhanced angiogenesis when compared with the control and ChGelPBS groups. The anti-fibrotic effects following MSC-Exo treatment were further found to be associated with the anti-inflammatory M2 polarization of the resident macrophages, especially within the M2b subset characterized by the reduced TGFβ1 secretion, which sufficiently inhibited inflammation and prevented the activation of myofibroblast with less collagen production at the early stage after ESD. Moreover, the abundant expression of exosomal MFGE8 was identified to be involved in the transition of the M2b-macrophage subset through the activation of MFGE8/STAT3/Arg1 axis.

Conclusions

Our study demonstrates that exosomal MFGE8 significantly promotes the polarization of the M2b-macrophage subset, consequently reducing collagen deposition. These findings suggest a promising potential for MSC-Exo therapy in preventing the development of esophageal stricture after near-circumferential ESD.

Graphical Abstract
Postoperative orthostatic intolerance following fast-track unicompartmental knee arthroplasty: incidence and hemodynamics—a prospective observational cohort study
Authors: Hristovska Ana-Marija , Andersen Louise B. , Uldall-Hansen Bodil , Kehlet Henrik , Troelsen Anders , Gromov Kirill , Foss Nicolai B.
Published: April 2024

Background

Early postoperative mobilization is essential for early functional recovery but can be inhibited by postoperative orthostatic intolerance (OI). Postoperative OI is common after major surgery, such as total knee arthroplasty (TKA). However, limited data are available after less extensive surgery, such as unicompartmental knee arthroplasty (UKA). We, therefore, investigated the incidence of OI as well as cardiovascular and tissue oxygenation responses during early mobilization after UKA.

Methods

This prospective single-centre observational study included 32 patients undergoing primary UKA. Incidence of OI and cardiovascular and tissue oxygenation responses during mobilization were evaluated preoperatively, at 6 and 24 h after surgery. Perioperative fluid balance, bleeding, surgery duration, postoperative hemoglobin, pain during mobilization and opioid usage were recorded.

Results

During mobilization at 6 h after surgery, 4 (14%, 95%CI 4–33%) patients experienced OI; however, no patients terminated the mobilization procedure prematurely. Dizziness and feeling of heat were the most common symptoms. OI was associated with attenuated systolic and mean arterial blood pressure responses in the sitting position (all p < 0.05). At 24 h after surgery, 24 (75%) patients had already been discharged, including three of the four patients with early OI. Only five patients were available for measurements, two of whom experienced OI; one terminated the mobilization procedure due to intolerable symptoms. We observed no statistically significant differences in perioperative fluid balance, bleeding, surgery duration, postoperative hemoglobin, pain, or opioid usage between orthostatic intolerant and tolerant patients.

Conclusions

The incidence of orthostatic intolerance after fast-track unicompartmental knee arthroplasty is low (~ 15%) and is associated with decreased orthostatic pressure responses. Compared to the previously described orthostatic intolerance incidence of ~ 40% following total knee arthroplasty, early orthostatic intolerance is uncommon after unicompartmental knee arthroplasty, suggesting a procedure-specific component.

Trial registration: Prospectively registered at ClinicalTrials.gov; registration number: NCT04195360, registration date: 13.12.2019.
Mid-term results of cemented hip arthroplasties through the direct anterior approach in the lateral decubitus position: a retrospective cohort study
Authors: Melman Wietse P.R. , Ettema Harmen B. , Edens Mireille A. , Verheyen Cees C.P.M.
Published: April 2024

Background

Reports show a high complication rate when starting with the Direct Anterior Approach (DAA) in a supine position for hip arthroplasty. The DAA with the patient in lateral decubitus position may avoid this problem because it supposedly provides better visibility, especially on the femoral side. However, this approach did show a rather high complication rate during the adoption of the approach at 1 year follow up in our previous report. We were interested what the overall 7 year survival estimate would be and whether improvement could be seen with growing experience.

Methods

A cohort of patients undergoing total hip arthroplasty right from the start of applying the DAA in lateral decubitus position was analysed.

Results

In total 175 hip prostheses (162 patients) were evaluated. The 7-year survival estimate was 95.1%, 95 CI: 91.8–98.4%. In 6 of 8 revisions there was aseptic loosening of the stem. By dividing the cohort into 3 consecutive groups in time we did not see a significantly improving revision rate.

Conclusions

In our experience, the adoption of the direct anterior approach in lateral decubitus position caused a relatively low 7-year survival estimate without an apparent decrease with growing experience, however given the low number of cases further research is needed to investigate the long-term risk of adopting a new approach.
The impact of an anesthesia residency teaching service on anesthesia-controlled time and postsurgical patient outcomes: a retrospective observational study on 15,084 surgical cases
Authors: Lynch Davene , Mongan Paul D. , Hoefnagel Amie L.
Published: April 2024

Background

Limited data exists regarding the impact of anesthesia residents on operating room efficiency and patient safety outcomes. This investigation hypothesized that supervised anesthesiology residents do not increase anesthesia-controlled or prolonged extubation times compared to supervised certified registered nurse anesthetists (CRNA)/certified anesthesiologist assistants (CAA) or anesthesiologists working independently. Secondary objectives included differences in critical outcomes such as intraoperative hypotension, cardiac and pulmonary complications, acute kidney injury, and mortality.

Methods

This retrospective single-center 24-month (January 1, 2020- December 31, 2021) cohort focused on primary outcomes of anesthesia-controlled times and prolonged extubation (>15 min) with additional assessment of secondary patient outcomes in adult patients having general anesthesia with an endotracheal tube or laryngeal mask airway for elective non-cardiac surgery. The study excluded sedation, obstetric, endoscopic, ophthalmology, and non-operating room procedures. Procedures were divided into three groups: anesthesiologists working solo, anesthesiologists supervising residents, or anesthesiologists supervising CRNA/CAAs. After univariate analysis, multivariable models were constructed to control for the univariate cofactor differences in the primary and secondary outcomes.

Results

A total of 15,084 surgical cases met the inclusion criteria for this study for the three different care models: solo anesthesiologists (1,204 cases), anesthesiologist/resident pairing (3,146 cases), and anesthesiologist/CRNA/CAA (14,040 cases). Before multivariate analysis, the resident group exhibited longer anesthesia-controlled times (median, [interquartile range], 26.1 [21.7–32.0], p < 0.001), compared to CRNA/CAA (23.9 [19.7–29.5]), and attending-only surgical cases (21.0 [17.9–25.4]). After adjusting for covariates in a general linear regression model (age, BMI, ASA classification, comorbidities, arterial line insertion, surgical service, and surgical location), there were no significant differences in the anesthesia-controlled times between the provider groups. Prolonged extubation times (>15 min) were significantly less common in the anesthesiologist-only group compared to the other groups (p < 0.001). Despite these time differences, there were no clinically significant differences among the groups in postoperative pulmonary or cardiac complications, renal impairment, or the 30-day mortality rate of patients.

Conclusion

Anesthesia residents do not increase anesthesia-controlled operating room times or adversely affect clinically relevant patient outcomes compared to anesthesiologists working independently or supervising certified registered nurse anesthetists or certified anesthesiologist assistants.
Liquid biopsy techniques and lung cancer: diagnosis, monitoring and evaluation
Authors: Ren Fei , Fei Qian , Qiu Kun , Zhang Yuanjie , Zhang Heyang , Sun Lei
Published: April 2024

Lung cancer stands as the most prevalent form of cancer globally, posing a significant threat to human well-being. Due to the lack of effective and accurate early diagnostic methods, many patients are diagnosed with advanced lung cancer. Although surgical resection is still a potential means of eradicating lung cancer, patients with advanced lung cancer usually miss the best chance for surgical treatment, and even after surgical resection patients may still experience tumor recurrence. Additionally, chemotherapy, the mainstay of treatment for patients with advanced lung cancer, has the potential to be chemo-resistant, resulting in poor clinical outcomes. The emergence of liquid biopsies has garnered considerable attention owing to their noninvasive nature and the ability for continuous sampling. Technological advancements have propelled circulating tumor cells (CTCs), circulating tumor DNA (ctDNA), extracellular vesicles (EVs), tumor metabolites, tumor-educated platelets (TEPs), and tumor-associated antigens (TAA) to the forefront as key liquid biopsy biomarkers, demonstrating intriguing and encouraging results for early diagnosis and prognostic evaluation of lung cancer. This review provides an overview of molecular biomarkers and assays utilized in liquid biopsies for lung cancer, encompassing CTCs, ctDNA, non-coding RNA (ncRNA), EVs, tumor metabolites, TAAs and TEPs. Furthermore, we expound on the practical applications of liquid biopsies, including early diagnosis, treatment response monitoring, prognostic evaluation, and recurrence monitoring in the context of lung cancer.
Nucleosome reorganisation in breast cancer tissues
Authors: Jacob Divya R. , Guiblet Wilfried M. , Mamayusupova Hulkar , Shtumpf Mariya , Ciuta Isabella , Ruje Luminita , Gretton Svetlana , Bikova Milena , Correa Clark , Dellow Emily , Agrawal Shivam P. , Shafiei Navid , Drobysevskaja Anastasija , Armstrong Chris M. , Lam Jonathan D. G. , Vainshtein Yevhen , Clarkson Christopher T. , Thorn Graeme J. , Sohn Kai , Pradeepa Madapura M. , Chandrasekharan Sankaran , Brooke Greg N. , Klenova Elena , Zhurkin Victor B. , Teif Vladimir B.
Published: April 2024

Background

Nucleosome repositioning in cancer is believed to cause many changes in genome organisation and gene expression. Understanding these changes is important to elucidate fundamental aspects of cancer. It is also important for medical diagnostics based on cell-free DNA (cfDNA), which originates from genomic DNA regions protected from digestion by nucleosomes.

Results

We have generated high-resolution nucleosome maps in paired tumour and normal tissues from the same breast cancer patients using MNase-assisted histone H3 ChIP-seq and compared them with the corresponding cfDNA from blood plasma. This analysis has detected single-nucleosome repositioning at key regulatory regions in a patient-specific manner and common cancer-specific patterns across patients. The nucleosomes gained in tumour versus normal tissue were particularly informative of cancer pathways, with ~ 20-fold enrichment at CpG islands, a large fraction of which marked promoters of genes encoding DNA-binding proteins. The tumour tissues were characterised by a 5–10 bp decrease in the average distance between nucleosomes (nucleosome repeat length, NRL), which is qualitatively similar to the differences between pluripotent and differentiated cells. This effect was correlated with gene activity, differential DNA methylation and changes in local occupancy of linker histone variants H1.4 and H1X.

Conclusions

Our study offers a novel resource of high-resolution nucleosome maps in breast cancer patients and reports for the first time the effect of systematic decrease of NRL in paired tumour versus normal breast tissues from the same patient. Our findings provide a new mechanistic understanding of nucleosome repositioning in tumour tissues that can be valuable for patient diagnostics, stratification and monitoring.
Saturnino Herrán’s portable murals: symbolism, material agency and conservation
Authors: Cano Nathael , de Lucio Oscar G. , Pérez Miguel , Mitrani Alejandro , Casanova Edgar , Ruvalcaba Sil José Luis
Published: April 2024

Alegoría de la construcción and Alegoría del trabajo were the first mural paintings commissioned to Saturnino Herrán (1887–1918), and they occupied a significant place in one of the most important schools in Mexico. The non-invasive methodology employed included different documentation phases and in situ multi-technique analyses. Hyperspectral imaging and digital radiography were used for a general characterization of the materials and were complemented with localized analyses by portable microscopy, and XRF and FORS spectroscopies. A complete identification of the original and the restoration palettes was achieved. Results from this work allowed us to understand the production context, painting technique, and conservation state, thus providing insights for the proper conservation of these murals, while also unveiling a new perception of the artist and his historical place among the muralist movement.
The long-chain flavodoxin FldX1 improves the biodegradation of 4-hydroxyphenylacetate and 3-hydroxyphenylacetate and counteracts the oxidative stress associated to aromatic catabolism in Paraburkholderia xenovorans
Authors: Rodríguez-Castro Laura , Durán Roberto E. , Méndez Valentina , Dorochesi Flavia , Zühlke Daniela , Riedel Katharina , Seeger Michael
Published: April 2024

Background

Bacterial aromatic degradation may cause oxidative stress. The long-chain flavodoxin FldX1 of Paraburkholderia xenovorans LB400 counteracts reactive oxygen species (ROS). The aim of this study was to evaluate the protective role of FldX1 in P. xenovorans LB400 during the degradation of 4-hydroxyphenylacetate (4-HPA) and 3-hydroxyphenylacetate (3-HPA).

Methods

The functionality of FldX1 was evaluated in P. xenovorans p2-fldX1 that overexpresses FldX1. The effects of FldX1 on P. xenovorans were studied measuring growth on hydroxyphenylacetates, degradation of 4-HPA and 3-HPA, and ROS formation. The effects of hydroxyphenylacetates (HPAs) on the proteome (LC–MS/MS) and gene expression (qRT-PCR) were quantified. Bioaugmentation with strain p2-fldX1 of 4-HPA-polluted soil was assessed, measuring aromatic degradation (HPLC), 4-HPA-degrading bacteria, and plasmid stability.

Results

The exposure of P. xenovorans to 4-HPA increased the formation of ROS compared to 3-HPA or glucose. P. xenovorans p2-fldX1 showed an increased growth on 4-HPA and 3-HPA compared to the control strain WT-p2. Strain p2-fldX1 degraded faster 4-HPA and 3-HPA than strain WT-p2. Both WT-p2 and p2-fldX1 cells grown on 4-HPA displayed more changes in the proteome than cells grown on 3-HPA in comparison to glucose-grown cells. Several enzymes involved in ROS detoxification, including AhpC2, AhpF, AhpD3, KatA, Bcp, CpoF1, Prx1 and Prx2, were upregulated by hydroxyphenylacetates. Downregulation of organic hydroperoxide resistance (Ohr) and DpsA proteins was observed. A downregulation of the genes encoding scavenging enzymes (katE and sodB), and gstA and trxB was observed in p2-fldX1 cells, suggesting that FldX1 prevents the antioxidant response. More than 20 membrane proteins, including porins and transporters, showed changes in expression during the growth of both strains on hydroxyphenylacetates. An increased 4-HPA degradation by recombinant strain p2-fldX1 in soil microcosms was observed. In soil, the strain overexpressing the flavodoxin FldX1 showed a lower plasmid loss, compared to WT-p2 strain, suggesting that FldX1 contributes to bacterial fitness. Overall, these results suggest that recombinant strain p2-fldX1 is an attractive bacterium for its application in bioremediation processes of aromatic compounds.

Conclusions

The long-chain flavodoxin FldX1 improved the capability of P. xenovorans to degrade 4-HPA in liquid culture and soil microcosms by protecting cells against the degradation-associated oxidative stress.
Glutaminolysis regulates endometrial fibrosis in intrauterine adhesion via modulating mitochondrial function
Authors: Chen Pei , Ye Chaoshuang , Huang Yunke , Xu Bingning , Wu Tianyu , Dong Yuanhang , Jin Yang , Zhao Li , Hu Changchang , Mao Jingxia , Wu Ruijin
Published: April 2024

Background

Endometrial fibrosis, a significant characteristic of intrauterine adhesion (IUA), is caused by the excessive differentiation and activation of endometrial stromal cells (ESCs). Glutaminolysis is the metabolic process of glutamine (Gln), which has been implicated in multiple types of organ fibrosis. So far, little is known about whether glutaminolysis plays a role in endometrial fibrosis.

Methods

The activation model of ESCs was constructed by TGF-β1, followed by RNA-sequencing analysis. Changes in glutaminase1 (GLS1) expression at RNA and protein levels in activated ESCs were verified experimentally. Human IUA samples were collected to verify GLS1 expression in endometrial fibrosis. GLS1 inhibitor and glutamine deprivation were applied to ESCs models to investigate the biological functions and mechanisms of glutaminolysis in ESCs activation. The IUA mice model was established to explore the effect of glutaminolysis inhibition on endometrial fibrosis.

Results

We found that GLS1 expression was significantly increased in activated ESCs models and fibrotic endometrium. Glutaminolysis inhibition by GLS1 inhibitor bis-2-(5-phenylacetamido-1,2,4-thiadiazol-2-yl) ethyl sulfide (BPTES or glutamine deprivation treatment suppressed the expression of two fibrotic markers, α-SMA and collagen I, as well as the mitochondrial function and mTORC1 signaling in ESCs. Furthermore, inhibition of the mTORC1 signaling pathway by rapamycin suppressed ESCs activation. In IUA mice models, BPTES treatment significantly ameliorated endometrial fibrosis and improved pregnancy outcomes.

Conclusion

Glutaminolysis and glutaminolysis-associated mTOR signaling play a role in the activation of ESCs and the pathogenesis of endometrial fibrosis through regulating mitochondrial function. Glutaminolysis inhibition suppresses the activation of ESCs, which might be a novel therapeutic strategy for IUA.
Computer-aided analysis of radiological images for cancer diagnosis: performance analysis on benchmark datasets, challenges, and directions
Authors: Alyami Jaber
Published: April 2024

Radiological image analysis using machine learning has been extensively applied to enhance biopsy diagnosis accuracy and assist radiologists with precise cures. With improvements in the medical industry and its technology, computer-aided diagnosis (CAD) systems have been essential in detecting early cancer signs in patients that could not be observed physically, exclusive of introducing errors. CAD is a detection system that combines artificially intelligent techniques with image processing applications thru computer vision. Several manual procedures are reported in state of the art for cancer diagnosis. Still, they are costly, time-consuming and diagnose cancer in late stages such as CT scans, radiography, and MRI scan. In this research, numerous state-of-the-art approaches on multi-organs detection using clinical practices are evaluated, such as cancer, neurological, psychiatric, cardiovascular and abdominal imaging. Additionally, numerous sound approaches are clustered together and their results are assessed and compared on benchmark datasets. Standard metrics such as accuracy, sensitivity, specificity and false-positive rate are employed to check the validity of the current models reported in the literature. Finally, existing issues are highlighted and possible directions for future work are also suggested.
Vegetation, fuels, and fire-behavior responses to linear fuel-break treatments in and around burned sagebrush steppe: are we breaking the grass-fire cycle?
Authors: Germino Matthew J. , Price Samuel “Jake” , Prichard Susan J.
Published: April 2024

Background

Linear fuel breaks are being implemented to moderate fire behavior and improve wildfire containment in semiarid landscapes such as the sagebrush steppe of North America, where extensive losses in perennial vegetation and ecosystem functioning are resulting from invasion by exotic annual grasses (EAGs) that foster large and recurrent wildfires. However, fuel-break construction can also pose EAG invasion risks, which must be weighed against the intended fire-moderation benefits of the treatments. We investigated how shrub reductions (mowing, cutting), pre-emergent EAG-herbicides, and/or drill seedings of fire-resistant perennial bunchgrasses (PBGs) recently applied to create a large fuel-break system affected native and exotic plant abundances and their associated fuel loading and predicted fire behavior.

Results

In heavily EAG-invaded areas, herbicides reduced EAG and total herbaceous cover without affecting PBGs for 2–3 years and reduced predicted fire behavior for 1 year (from the Fuel Characteristic Classification System). However, surviving post-herbicide EAG cover was still > 30%, which was sufficient fuel to exceed the conventional 1.2-m-flame length (FL) threshold for attempting wildfire suppression with hand tools. In less invaded shrubland, shrub reduction treatments largely reduced shrub cover and height by ~ half without increasing EAGs, but then redistributed the wood to ground level and increased total herbaceous cover. Herbicides and/or drill seeding after shrub reductions did not affect EAG cover, although drill seedings increased PBG cover and exotic forbs (e.g., Russian thistle). Fire behavior was predicted to be moderated in only one of the many yearly observations of the various shrub-reduction treatment combinations. Over all treatments and years, FLs were predicted to exceed 1.2 m in 13% of simulations under average (11 km h−1) or high (47 km h−1) wind speed conditions and exceed the 3.4-m threshold for uncontrollable fire in 11% of simulations under high-wind speeds only.

Conclusions

Predicted fire-moderation benefits over the first 4 years of fuel break implementation were modest and variable, but, generally, increases in EAGs and their associated fire risks were not observed. Nonetheless, ancillary evidence from shrublands would suggest that treatment-induced shifts from shrub to herbaceous fuel dominance are expected to improve conditions for active fire suppression in ways not readily represented in available fire models.
Perspectives of healthcare professionals on the use of immersive virtual reality in teenage and young adult oncology: a qualitative interview study
Authors: Deighan Mairi Therese , Simpson Daniel , Brigden Amberly , Cater Kirsten
Published: April 2024

Background

Applications of Virtual Reality (VR) in healthcare continue to grow at a rapid pace, yet the challenges and opportunities associated with VR from the perspective of clinicians and healthcare staff remain underexplored, particularly in the context of Teenage and Young Adult (TYA) oncology. This study aims to bridge this knowledge gap by interviewing clinicians and staff regarding the use of VR in TYA cancer care.

Method

Fourteen semi-structured qualitative interviews were conducted with a diverse range of healthcare professionals working in TYA oncology within the United Kingdom including oncologists, hematologists, clinical nurse specialists, clinical psychologists, radiographers, play specialists, clinical social workers and youth support coordinators. Data were analysed thematically with three themes and ten sub-themes developed.

Results

The findings reveal that TYA healthcare professionals are optimistic and enthusiastic about the potential of using VR to enhance cancer care for teenagers and young adults. However, they also expressed notable concerns related to the practical implementation of VR in clinical settings. These concerns included infection and safety risks, privacy and security concerns, cost implications, storage requirements and staff burden. Clinicians and staff proposed valuable applications for VR in TYA oncology specifically in patient distraction, physiotherapy, procedure preparation, and the delivery of psychotherapy, including acceptance and commitment therapy.

Conclusions

This research has demonstrated that despite an enthusiasm to adopt VR in TYA oncology there are still many challenges, both practical and ethical, that must be addressed to enable the successful implementation of VR in hospitals within the UK. Further research into applications of VR for TYA oncology is warranted, particularly in areas of psychology, physiotherapy, and procedure preparation.
Wavefield decomposition for viscoelastic anisotropic media
Authors: Hao Qi , Tsvankin Ilya
Published: April 2024

Separating wave modes on seismic records is an essential step in imaging of multicomponent seismic data. Viscoelastic anisotropic models provide a realistic description of subsurface formations that exhibit anisotropy of velocity and attenuation. However, mode separation has not been extended to viscoelastic anisotropic media yet. Here, we propose an efficient approach to wavefield decomposition that takes velocity and attenuation anisotropy into account. Our algorithm operates in the frequency-wavenumber domain and, therefore, is suitable for general dissipative models. We present exact equations for wavefield decomposition in arbitrarily anisotropic attenuative homogeneous media. Then the proposed approach is applied to viscoelastic constant-[Formula: see text] VTI (transversely isotropic with a vertical symmetry axis) models. Numerical examples demonstrate the accuracy and efficiency of our approach for piecewise-homogeneous media characterized by pronounced velocity and attenuation anisotropy.
President's Page: CCUS: Challenges and opportunities
Authors: Zhou Shan
Published: April 2024

Carbon capture, usage, and storage (CCUS) technologies can move carbon dioxide produced from fossil-fueled power plants and other industrial facilities to geologic storage sites at a distance. Due to the pressing societal need to reduce greenhouse gas emissions, CCUS has gained wide attention as a flexible, cost-effective, and rapid approach to climate change mitigation and energy security. CCUS is particularly appealing to industries for which decarbonizing has been historically challenging.
An artificial intelligence workflow for horizon volume generation from 3D seismic data
Authors: Abubakar Aria , Di Haibin , Li Zhun , Maniar Hiren , Zhao Tao
Published: April 2024

Horizon-based subsurface stratigraphic model building is a tedious process, especially in geologically complex areas where seismic data are contaminated with noise and thus are of weak and discontinuous reflectors. Seismic interpreters usually use stratal (proportional) slices to approximately inspect 3D seismic data along seismic reflectors yet to be interpreted. We introduce an artificial intelligence workflow consisting of three deep learning steps to provide a conditioned seismic image that is easier to interpret, a stratigraphic model that outlines major formations, and moreover a relative geologic time volume that allows us to automatically extract an infinite number of horizons along any seismic reflectors within a seismic cube. Depending on the availability of interpreters, the proposed workflow can either run fully unsupervised without human inputs or using sparse horizon interpretation as constraints to further improve the quality of extracted horizons, providing flexibility in both efficiency and quality. Starting from only seismic images and a few key horizons interpreted on very sparse seismic lines, we demonstrate the workflow to generate a stack of complete horizons covering the entire seismic volume from offshore Australia.
Reviews
Authors: Aitken Julie
Published: April 2024

Earth's Core: Geophysics of a Planet's Deepest Interior, by Vernon F. Cormier, Michael I. Bergman, and Peter L. Olson, 2021 Remote Sensing Digital Image Analysis, by John A. Richards, 2022
Challenges to mapping and defining m6A function in viral RNA
Authors: Horner Stacy M. , Thompson Matthew G.
Published: April 2024

Viral RNA molecules contain multiple layers of regulatory information. This includes features beyond the primary sequence, such as RNA structures and RNA modifications, including N6-methyladenosine (m6A). Many recent studies have identified the presence and location of m6A in viral RNA and have found diverse regulatory roles for this modification during viral infection. However, to date, viral m6A mapping strategies have limitations that prevent a complete understanding of the function of m6A on individual viral RNA molecules. While m6A sites have been profiled on bulk RNA from many viruses, the resulting m6A maps of viral RNAs described to date present a composite picture of m6A across viral RNA molecules in the infected cell. Thus, for most viruses, it is unknown if unique viral m6A profiles exist throughout infection, nor if they regulate specific viral life cycle stages. Here, we describe several challenges to defining the function of m6A in viral RNA molecules and provide a framework for future studies to help in the understanding of how m6A regulates viral infection.
Adenosine deaminases that act on RNA, then and now
Authors: Bass Brenda L.
Published: April 2024

In this article, I recount my memories of key experiments that led to my entry into the RNA editing/modification field. I highlight initial observations made by the pioneers in the ADAR field, and how they fit into our current understanding of this family of enzymes. I discuss early mysteries that have now been solved, as well as those that still linger. Finally, I discuss important, outstanding questions and acknowledge my hope for the future of the RNA editing/modification field.
Τέχνη, περιβάλλον και εκπαίδευση
Authors: Φραγγίδης Μανώλης
Published: April 2024

Η παρούσα διατριβή εντάσσεται στην προσπάθεια διερεύνησης και ανάδειξης της σημασίας της σύνδεσης Τέχνης, Εκπαίδευσης και Περιβάλλοντος, καθώς και τη θετική τους αλληλεπίδραση, στο πλαίσιο της μη-τυπικής Περιβαλλοντικής Εκπαίδευσης Για το Περιβάλλον. Πρόκειται για μελέτη περίπτωσης που αφορά σε μαθητές και μαθήτριες ελληνικού Γυμνασίου ορεινής κοινότητας της Ηπείρου. Με μεθοδολογική βάση τη θεμελιωμένη θεωρία, η παρούσα έρευνα συνδυάζει ποσοτικές και ποιοτικές μεθόδους συλλογής, μελέτης, επεξεργασίας και ανάλυσης δεδομένων που περιλαμβάνουν παρατήρηση, ημιδομημένες συνεντεύξεις, καθώς και συγκέντρωση και ανάλυση πρωτογενούς εικαστικού υλικού που δημιουργήθηκε από τους συμμετέχοντες. Αξιοποιώντας έναν τοπικό μύθο δρακοκτονίας, που λειτούργησε ως κομβικό σημείο σύνδεσης των συμμετεχόντων με τον οικείο χωρότοπο, σχεδιάστηκαν και υλοποιήθηκαν τρεις διδακτικές και μία εικαστική παρέμβαση. Στόχος των παρεμβάσεων και αποτέλεσμα αυτών, όπως προκύπτει από την ανάλυση δεδομένων, ήταν η πρόκληση αλλαγών στις αντιλήψεις, τις στάσεις και τις συμπεριφορές των συμμετεχόντων προς μία οικοκεντρική και φιλοπεριβαλλοντική κατεύθυνση.