bluuebunny/crossref_metadata_2025 huggingface dataset:
Efficacy of Serial Ultrasonographic Examinations in Predicting Return to Play in Agility Dogs with Shoulder Lameness
Authors: Entani Maria Grazia , Franini Alessio , Dragone Ludovica , Barella Gabriele , De Rensis Fabio , Spattini Giliola
Published: December 2021

The aim of this study is to investigate the use of shoulder ultrasound as a method of predicting the likelihood of returning to competition in agility dogs with shoulder teno-muscular injuries after a standardised rehabilitation protocol. Thirty-two agility dogs with a clinical and ultrasonographic diagnosis of shoulder teno-muscular injury were included in a prospective study with physical and ultrasound examinations at the time of diagnosis (T0) and at two (T2), four (T4) and six (T6) months; during this period, the dogs received rehabilitation treatments. The endpoint of the study was to obtain information regarding participation in agility competitions 12 months after diagnosis, based on telephone interviews with the owners. The clinical lameness score (CLS) and the ultrasound lesion score (ULS) were used as outcome measurements. The CLS indicated partial recovery from a shoulder injury at T2 (78%), while the ULS indicated no satisfactory recovery at T2 in any patient. At 4 months, the CLS alone was not a valuable predictor of full recovery from a shoulder injury in agility dogs. Relative Risk indicated that, at T2, ultrasound was 23.8 times more valuable in identifying a shoulder lesion as compared to clinical lameness score (CLS), and it was 2.53 times more valuable at T4.
First Integrative Morphological and Genetic Characterization of Tremoctopus violaceussensu stricto in the Mediterranean Sea
Authors: Agus Blondine , Carbonara Pierluigi , Melis Riccardo , Cannas Rita , Carugati Laura , Cera Jacopo , Donnaloia Marilena , Mulas Antonello , Pais Antonio , Ruiu Stefano , Vinci Giuseppe , Cuccu Danila
Published: December 2021

An integrative approach based on morphological and genetic analyses was undertaken for the first time to confirm the species identification of Mediterranean samples belonging to the genus Tremoctopus. Sequences of two mtDNA genes (cytochrome c oxidase subunit (COI) and 16S) were generated for the first time from Mediterranean samples. Both the similarity-based identifications and tree-based methods indicated that three females can be identified as Tremoctopus violaceus sensu stricto in agreement with their morphological classifications. All Mediterranean sequences clustered with the sequences of Tremoctopus violaceus from the Gulf of Mexico and were clearly differentiated from the sequences attributed to T. gracilis and T. robsoni. The chromatic pattern of the web and some features of gill filaments, arms formula, stylets, radulae, beaks, and stomach contents were given for all the samples; 105,758, 20,140, and 11,237 oocytes were estimated in the mature, immature, and developing samples, respectively. The presence of four spermatangia inside the cavity of the maturing female suggested the ability of this species to mate before reaching full maturity with more partners. Age investigation using beaks, performed for the first time in T. violaceus and within the genus gave results consistent with the different sizes and maturity conditions of the samples.
Corticosterone Metabolite Concentration Is Not Related to Problem Solving in the Fawn-Footed Mosaic-Tailed Rat Melomys Cervinipes
Authors: Rowell Misha K. , Santymire Rachel M. , Rymer Tasmin L.
Published: December 2021

Animals can respond physiologically, such as by adjusting glucocorticoid hormone concentrations, to sudden environmental challenges. These physiological changes can then affect behavioural and cognitive responses. While the relationships between adrenocortical activity and behaviour and cognition are well documented, results are equivocal, suggesting species-specific responses. We investigated whether adrenocortical activity, measured using corticosterone metabolite concentration, was related to problem solving in an Australian rodent, the fawn-footed mosaic-tailed rat (Melomys cervinipes). Mosaic-tailed rats live in complex environments that are prone to disturbance, suggesting a potential need to solve novel problems, and have been found to show relationships between physiology and other behaviours. We measured problem solving using five food-baited puzzles (matchbox and cylinder in the home cage, and activity board with pillars to push, tiles to slide and levers to lift in an open field), and an escape-motivated obstruction task in a light/dark box. Faecal samples were collected from individuals during routine cage cleaning. Adrenocortical activity was evaluated non-invasively by measuring faecal corticosterone metabolites using an enzyme immunoassay, which was biochemically and biologically validated. Despite varying over time, adrenocortical activity was not significantly related to problem solving success or time spent interacting for any task. However, as adrenocortical activity is reflective of multiple physiological processes, including stress and metabolism, future studies should consider how other measures of physiology are also linked to problem solving.
Revisiting Antibiotic Resistance: Mechanistic Foundations to Evolutionary Outlook
Authors: Hasan Chowdhury M. , Dutta Debprasad , Nguyen An N. T.
Published: December 2021

Antibiotics are the pivotal pillar of contemporary healthcare and have contributed towards its advancement over the decades. Antibiotic resistance emerged as a critical warning to public wellbeing because of unsuccessful management efforts. Resistance is a natural adaptive tool that offers selection pressure to bacteria, and hence cannot be stopped entirely but rather be slowed down. Antibiotic resistance mutations mostly diminish bacterial reproductive fitness in an environment without antibiotics; however, a fraction of resistant populations ‘accidentally’ emerge as the fittest and thrive in a specific environmental condition, thus favouring the origin of a successful resistant clone. Therefore, despite the time-to-time amendment of treatment regimens, antibiotic resistance has evolved relentlessly. According to the World Health Organization (WHO), we are rapidly approaching a ‘post-antibiotic’ era. The knowledge gap about antibiotic resistance and room for progress is evident and unified combating strategies to mitigate the inadvertent trends of resistance seem to be lacking. Hence, a comprehensive understanding of the genetic and evolutionary foundations of antibiotic resistance will be efficacious to implement policies to force-stop the emergence of resistant bacteria and treat already emerged ones. Prediction of possible evolutionary lineages of resistant bacteria could offer an unswerving impact in precision medicine. In this review, we will discuss the key molecular mechanisms of resistance development in clinical settings and their spontaneous evolution.
The Engineered Antibiotic Peptide PLG0206 Eliminates Biofilms and Is a Potential Treatment for Periprosthetic Joint Infections
Authors: Huang David , Pachuda Nicholas , Sauer John Michael , Dobbins Dessie , Steckbeck Jonathan
Published: December 2021

Antimicrobial peptides (AMPs) have recently gained attention for their potential to treat diseases related to bacterial and viral infections, as many traditional antimicrobial drugs have reduced efficacy in treating these infections due to the increased prevalence of drug-resistant pathogens. PLG0206, an engineered cationic antibiotic peptide that is 24 residues long, has been designed to address some limitations of other natural AMPs, such as toxicity and limited activity due to pH and ion concentrations. Nonclinical studies have shown that PLG0206 is highly selective for targeting bacterial cells and is not toxic to human blood cells. Antibiofilm experiments demonstrated that PLG0206 is effective at reducing both biotic and abiotic biofilm burdens following direct biofilm contact. PLG0206 has rapid and broad-spectrum activity against both Gram-positive and Gram-negative bacteria that are implicated as etiologic agents in periprosthetic joint infections, including multidrug-resistant ESKAPE pathogens and colistin-resistant isolates. A recent first-in-human study demonstrated that PLG0206 is well tolerated and safe as an intravenous infusion in healthy volunteers. Studies are planned to determine the efficacy of PLG0206 in patients for the treatment of periprosthetic joint infections. This review summarizes the chemistry, pharmacology, and microbiology of PLG0206 and explores its current preclinical, clinical, and regulatory status.
NDM Production as a Dominant Feature in Carbapenem-Resistant Enterobacteriaceae Isolates from a Tertiary Care Hospital
Authors: Uddin Fakhur , Imam Syed Hadi , Khan Saeed , Khan Taseer Ahmed , Ahmed Zulfiqar , Sohail Muhammad , Elnaggar Ashraf Y. , Fallatah Ahmed M. , El-Bahy Zeinhom M.
Published: December 2021

The worldwide spread and increasing prevalence of carbapenem-resistant Enterobacteriaceae (CRE) is of utmost concern and a problem for public health. This resistance is mainly conferred by carbapenemase production. Such strains are a potential source of outbreaks in healthcare settings and are associated with high rates of morbidity and mortality. In this study, we aimed to determine the dominance of NDM-producing Enterobacteriaceae at a teaching hospital in Karachi. A total of 238 Enterobacteriaceae isolates were collected from patients admitted to Jinnah Postgraduate Medical Centre (Unit 4) in Karachi, Pakistan, a tertiary care hospital. Phenotypic and genotypic methods were used for detection of metallo-β-lactamase. Out of 238 isolates, 52 (21.8%) were CRE and 50 isolates were carbapenemase producers, as determined by the CARBA NP test; two isolates were found negative for carbapenemase production by CARB NP and PCR. Four carbapenemase-producing isolates phenotypically appeared negative for metallo-β-lactamase (MBL). Of the 52 CRE isolates, 46 (88.46%) were blaNDM positive. Most of the NDM producers were Klebsiella pneumoniae, followed by Enterobacter cloacae and Escherichia coli. In all the NDM-positive isolates, the blaNDM gene was found on plasmid. These isolates were found negative for the VIM and IPM MBLs. All the CRE and carbapenem-sensitive isolates were sensitive to colistin. It is concluded that the NDM is the main resistance mechanism against carbapenems and is dominant in this region.
Cell Models and Omics Techniques for the Study of Nonalcoholic Fatty Liver Disease: Focusing on Stem Cell-Derived Cell Models
Authors: Pelechá María , Villanueva-Bádenas Estela , Timor-López Enrique , Donato María Teresa , Tolosa Laia
Published: December 2021

Nonalcoholic fatty liver disease (NAFLD) is now the leading cause of chronic liver disease in western countries. The molecular mechanisms leading to NAFLD are only partially understood, and effective therapeutic interventions are clearly needed. Therefore, preclinical research is required to improve knowledge about NAFLD physiopathology and to identify new therapeutic targets. Primary human hepatocytes, human hepatic cell lines, and human stem cell-derived hepatocyte-like cells exhibit different hepatic phenotypes and have been widely used for studying NAFLD pathogenesis. In this paper, apart from employing the different in vitro cell models for the in vitro assessment of NAFLD, we also reviewed other approaches (metabolomics, transcriptomics, and high-content screening). We aimed to summarize the characteristics of different cell types and methods and to discuss their major advantages and disadvantages for NAFLD modeling.
Clotting Dysfunction in Sepsis: A Role for ROS and Potential for Therapeutic Intervention
Authors: Lopes-Pires Maria Elisa , Frade-Guanaes Jéssica Oliveira , Quinlan Gregory J.
Published: December 2021

Sepsis is regarded as one of the main causes of death among the critically ill. Pathogen infection results in a host-mediated pro-inflammatory response to fight infection; as part of this response, significant endogenous reactive oxygen (ROS) and nitrogen species (RNS) production occurs, instigated by a variety of sources, including activated inflammatory cells, such as neutrophils, platelets, and cells from the vascular endothelium. Inflammation can become an inappropriate self-sustaining and expansive process, resulting in sepsis. Patients with sepsis often exhibit loss of aspects of normal vascular homeostatic control, resulting in abnormal coagulation events and the development of disseminated intravascular coagulation. Diagnosis and treatment of sepsis remain a significant challenge for healthcare providers globally. Targeting the drivers of excessive oxidative/nitrosative stress using antioxidant treatments might be a therapeutic option. This review focuses on the association between excessive oxidative/nitrosative stress, a common feature in sepsis, and loss of homeostatic control at the level of the vasculature. The literature relating to potential antioxidants is also described.
N-Acetylcysteine Reverses Monocrotophos Exposure-Induced Hepatic Oxidative Damage via Mitigating Apoptosis, Inflammation and Structural Changes in Rats
Authors: Singh Jagjeet , Phogat Annu , Prakash Chandra , Chhikara Sunil Kumar , Singh Sandeep , Malik Vinay , Kumar Vijay
Published: December 2021

Oxidative stress-mediated tissue damage is primarily involved in hepatic injuries and dysfunctioning. Natural antioxidants have been shown to exert hepatoprotective, anti-inflammatory and antiapoptotic properties. The present study evaluated the effect of N-acetylcysteine (NAC) against monocrotophos (MCP) exposure-induced toxicity in the rat liver. Albino Wistar rats were divided into four groups: (1) control, (2) NAC-treated, (3) MCP-exposure, (4) NAC and MCP-coexposure group. The dose of MCP (0.9 mg/kg b.wt) and NAC (200 mg/kg b.wt) were administered orally for 28 days. Exposure to MCP caused a significant increase in lipid peroxidation, protein oxidation and decreased glutathione content along with the depletion of antioxidant enzyme activities. Further MCP exposure increased pro-inflammatory cytokines levels and upregulated Bax and Caspase-3 expressions. MCP exposure also caused an array of structural alternations in liver tissue, as depicted by the histological and electron microscopic analysis. Thepretreatment of NAC improved glutathione content, restored antioxidant enzyme activities, prevented oxidation of lipids and proteins, decreased pro-inflammatory cytokines levels and normalized apoptotic protein expression. Treatment of NAC also prevented histological and ultrastructural alternations. Thus, the study represents the therapeutic efficacy and antioxidant potential of NAC against MCP exposure in the rat liver.
Oxidative Stress Is a Key Modulator in the Development of Nonalcoholic Fatty Liver Disease
Authors: Ma Yuanqiang , Lee Gyurim , Heo Su-Young , Roh Yoon-Seok
Published: December 2021

Nonalcoholic fatty liver disease (NAFLD) is the most common chronic liver disease worldwide, and scientific studies consistently report that NAFLD development can be accelerated by oxidative stress. Oxidative stress can induce the progression of NAFLD to NASH by stimulating Kupffer cells, hepatic stellate cells, and hepatocytes. Therefore, studies are underway to identify the role of antioxidants in the treatment of NAFLD. In this review, we have summarized the origins of reactive oxygen species (ROS) in cells, the relationship between ROS and NAFLD, and have discussed the use of antioxidants as therapeutic agents for NAFLD.
Gallic Acid Improves Diabetic Steatosis by Downregulating MicroRNA-34a-5p through Targeting NFE2L2 Expression in High-Fat Diet-Fed db/db Mice
Authors: Lee Ang-Tse , Yang Mon-Yuan , Lee Yi-Ju , Yang Tzu-Wei , Wang Chi-Chih , Wang Chau-Jong
Published: December 2021

Type 2 diabetes mellitus (T2DM) has become epidemic worldwide and is strongly associated with nonalcoholic fatty liver disease (NAFLD). The molecular mechanisms of microRNAs in NAFLD and T2DM development and the corresponding therapies remain unclear. We performed microRNA microarray validation to determine whether hepatic miR-34a-5p was significantly upregulated in db/db mice fed with a high-fat diet (HFD), a mouse model of T2DM with steatohepatitis. The potential role of miR-34a-5p and gallic acid (GA) in regulating hepatic lipid metabolism and diabetic steatosis was explored. GA improved the activities of antioxidant enzymes and suppressed lipid accumulation in the HFD-induced steatotic liver of db/db mice. In vitro, the silencing of miR-34a-5p in hepatocyte HepG2 cells ameliorated high glucose + oleic acid/palmitic acid mixture–induced accumulation of cellular triglycerides. We identified nuclear factor erythroid-derived 2-like 2 (NFE2L2) as a direct target of miR-34a-5p. Reduction in intracellular triglyceride and the expression levels of sterol regulatory element-binding protein 1 and fatty acid synthase by GA were mediated by the inhibition of miR-34a-5p expression in HepG2 cells. The findings suggest that GA improves hepatic lipogenesis by downregulating miR-34a-5p by suppressing NFE2L2 expression, indicating the potential therapeutic role of GA or an NFE2L2-activating agent in diabetic fatty liver disease.
Typical 2-Cys Peroxiredoxins as a Defense Mechanism against Metal-Induced Oxidative Stress in the Solitary Ascidian Ciona robusta
Authors: Drago Laura , Ferro Diana , Bakiu Rigers , Ballarin Loriano , Santovito Gianfranco
Published: December 2021

Typical 2-Cys peroxiredoxins (2-Cys Prdxs) are proteins with antioxidant properties belonging to the thioredoxin peroxidase family. With their peroxidase activity, they contribute to the homeostatic control of reactive oxygen species (ROS) and, therefore, participate in various physiological functions, such as cell proliferation, differentiation, and apoptosis. Although Prdxs have been shown to be potential biomarkers for monitoring aquatic environments, minimal scientific attention has been devoted to describing their molecular architecture and function in marine invertebrates. Our study aims to clarify the protective role against stress induced by exposure to metals (Cu, Zn, and Cd) of three Prdxs (Prdx2, Prdx3, and Prdx4) in the solitary ascidian Ciona robusta, an invertebrate chordate. Here, we report a detailed pre- and post-translational regulation of the three Prdx isoforms. Data on intestinal mRNA expression, provided by qRT-PCR analyses, show a generalized increase for Prdx2, -3, and -4, which is correlated to metal accumulation. Furthermore, the increase in tissue enzyme activity observed after Zn exposure is slower than that observed with Cu and Cd. The obtained results increase our knowledge of the evolution of anti-stress proteins in invertebrates and emphasize the importance of the synthesis of Prdxs as an efficient way to face adverse environmental conditions.
Cultivation of Cells in a Physiological Plasmax Medium Increases Mitochondrial Respiratory Capacity and Reduces Replication Levels of RNA Viruses
Authors: Golikov Michail V. , Karpenko Inna L. , Lipatova Anastasiya V. , Ivanova Olga N. , Fedyakina Irina T. , Larichev Viktor F. , Zakirova Natalia F. , Leonova Olga G. , Popenko Vladimir I. , Bartosch Birke , Kochetkov Sergey N. , Smirnova Olga A. , Ivanov Alexander V.
Published: December 2021

Changes in metabolic pathways are often associated with the development of various pathologies including cancer, inflammatory diseases, obesity and metabolic syndrome. Identification of the particular metabolic events that are dysregulated may yield strategies for pharmacologic intervention. However, such studies are hampered by the use of classic cell media that do not reflect the metabolite composition that exists in blood plasma and which cause non-physiological adaptations in cultured cells. In recent years two groups presented media that aim to reflect the composition of human plasma, namely human plasma-like medium (HPLM) and Plasmax. Here we describe that, in four different mammalian cell lines, Plasmax enhances mitochondrial respiration. This is associated with the formation of vast mitochondrial networks and enhanced production of reactive oxygen species (ROS). Interestingly, cells cultivated in Plasmax displayed significantly less lysosomes than when any standard media were used. Finally, cells cultivated in Plasmax support replication of various RNA viruses, such as hepatitis C virus (HCV) influenza A virus (IAV), severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) and several others, albeit at lower levels and with delayed kinetics. In conclusion, studies of metabolism in the context of viral infections, especially those concerning mitochondria, lysosomes, or redox systems, should be performed in Plasmax medium.
Antioxidative Effect of Sage (Salvia officinalis L.) Macerate as “Green Extract” in Inhibiting the Oxidation of Fish Oil
Authors: Hrebień-Filisińska Agnieszka M. , Bartkowiak Artur
Published: December 2021

The aim of the study was to assess the antioxidant effect of concentrated oil macerate of sage (M) as a “green extract” in inhibiting the oxidation of Fish Oil (FO). In the homogenization-assisted maceration process, FO was used as a solvent for the sage active substances to produce M, which was then added to FO (25% w/w) and evaluated for its effect by monitoring the level of oxidation during refrigerated and room temperature storage. The macerate also examined polyphenols, plant pigments, DPPH antioxidant potential, oxidation level and sensory quality. It was shown that the maceration process made it possible to obtain aromatized M, containing polyphenols (carnosic acid, carnosol) and pigments, but with an increased level of peroxides, free fatty acids, compared to the control oil. M showed antioxidant properties and inhibited FO oxidation. It showed the best efficiency in FO during refrigerated storage, in the third month it reduced the level of peroxides by about 9 times, compared to the control. M retains unchanged quality at refrigerated temperature for up to 3 months. Sage macerates are “green extracts” that can be used as effective natural antioxidant additives, following preparation improvements to reduce the amount of peroxide formed.
Design and Experimental Research of Variable Formula Fertilization Control System Based on Prescription Diagram
Authors: Niu Kang , Bai Shenghe , Zhou Liming , Zhao Bo , Liu Lijin , Yuan Yanwei , Yang Deqiu , Xiong Shi , Zhang Weipeng
Published: December 2021

In order to solve the problems such as the inability to automatically mix a variety of solid fertilizers and the unreasonable fertilizer amount, improve fertilizer utilization, and reduce production costs, this study designs a variable formula fertilization control system based on a prescription diagram, including pressure sensor, speed sensor, servo motor, fertilizer discharge actuator, Programmable Logic Controller (PLC controller), vehicle control terminal, etc. Based on pre-loaded soil prescription diagram and combining fertilizer pressure and ground wheel speed detection information, the system obtained a formula fertilization control strategy through calculation to realize the function of fast and automatic formula of nitrogen, phosphorus, and potassium fertilizers and precise variable fertilization. The experimental study on the performance of the variable formula fertilization control system showed the following: the measurement error range of the pressure sensor was 0.005~0.03%; the relationship between the motor speed and the amount of nitrogen, phosphorus, and potassium fertilizer discharged was calibrated. Three gears were established for the motor speed: low (10 r/min), medium (30 r/min), and high (50 r/min); the measurement accuracy of the speed sensor was above 98%. The test verified that the control accuracy of the variable formula fertilization system reached more than 95%, which met the requirements of fast automatic formula and precise variable fertilization and had good practicability and economy.
Experimental Methodology to Determine Thermal Conductivity of Nanofluids by Using a Commercial Transient Hot-Wire Device
Authors: Prado Jose I. , Calviño Uxía , Lugo Luis
Published: December 2021

The lack of a standard experimental procedure to determine thermal conductivity of fluids is noticeable in heat transfer processes from practical and fundamental perspectives. Since a wide variety of techniques have been used, reported literature data have huge discrepancies. A common practice is using manufactured thermal conductivity meters for nanofluids, which can standardize the measurements but are also somewhat inaccurate. In this study, a new methodology to perform reliable measurements with a recent commercial transient hot-wire device is introduced. Accordingly, some extensively studied fluids in the literature (water, ethylene glycol, ethylene glycol:water mixture 50:50 vol%, propylene glycol, and n-tetradecane) covering the range 0.100 to 0.700 W m−1 K−1 were used to check the device in the temperature range 283.15 to 333.15 K. Deviations between the collected data and the theoretical model, and repeatabilities and deviations between reported and literature values, were analyzed. Systematic deviations in raw data were found, and a correction factor depending on the mean thermal conductivity was proposed to operate with nanofluids. Considering all tested effects, the expanded (k = 2) uncertainty of the device was set as 5%. This proposed methodology was also checked with n-hexadecane and magnesium-oxide-based n-tetradecane nanofluids.
Virtual Reality-Based Parallel Coordinates Plots Enhanced with Explainable AI and Data-Science Analytics for Decision-Making Processes
Authors: Bobek Szymon , Tadeja Sławomir K. , Struski Łukasz , Stachura Przemysław , Kipouros Timoleon , Tabor Jacek , Nalepa Grzegorz J. , Kristensson Per Ola
Published: December 2021

We present a refinement of the Immersive Parallel Coordinates Plots (IPCP) system for Virtual Reality (VR). The evolved system provides data-science analytics built around a well-known method for visualization of multidimensional datasets in VR. The data-science analytics enhancements consist of importance analysis and a number of clustering algorithms including a novel SuMC (Subspace Memory Clustering) solution. These analytical methods were applied to both the main visualizations and supporting cross-dimensional scatter plots. They automate part of the analytical work that in the previous version of IPCP had to be done by an expert. We test the refined system with two sample datasets that represent the optimum solutions of two different multi-objective optimization studies in turbomachinery. The first one describes 54 data items with 29 dimensions (DS1), and the second 166 data items with 39 dimensions (DS2). We include the details of these methods as well as the reasoning behind selecting some methods over others.
Gaussian-Based Machine Learning Algorithm for the Design and Characterization of a Porous Meta-Material for Acoustic Applications
Authors: Casaburo Alessandro , Magliacano Dario , Petrone Giuseppe , Franco Francesco , De Rosa Sergio
Published: December 2021

The scope of this work is to consolidate research dealing with the vibroacoustics of periodic media. This investigation aims at developing and validating tools for the design and characterization of global vibroacoustic treatments based on foam cores with embedded periodic patterns, which allow passive control of acoustic paths in layered concepts. Firstly, a numerical test campaign is carried out by considering some perfectly rigid inclusions in a 3D-modeled porous structure; this causes the excitation of additional acoustic modes due to the periodic nature of the meta-core itself. Then, through the use of the Delany–Bazley–Miki equivalent fluid model, some design guidelines are provided in order to predict several possible sets of characteristic parameters (that is unit cell dimension and foam airflow resistivity) that, constrained by the imposition of the total thickness of the acoustic package, may satisfy the target functions (namely, the frequency at which the first Transmission Loss (TL) peak appears, together with its amplitude). Furthermore, when the Johnson–Champoux–Allard model is considered, a characterization task is performed, since the meta-material description is used in order to determine its response in terms of resonance frequency and the TL increase at such a frequency. Results are obtained through the implementation of machine learning algorithms, which may constitute a good basis in order to perform preliminary design considerations that could be interesting for further generalizations.
CBCT in Pediatric Dentistry: Awareness and Knowledge of Its Correct Use in Saudi Arabia
Authors: Bhardwaj Smita Singh , Alghamdi Sara , Almulhim Basim , Alassaf Abdullah , Almalki Abdullah , Bhardwaj Atul , Alzunaydi Ayoub
Published: December 2021

CBCT (Cone-beam computed tomography) is used for diagnosis, planning of treatment, and research. However, there are doubts and opinions regarding the use of CBCT for children and in pediatric dentistry. The knowledge about using this technique for pediatric patients is not clear to the dentists and some dental situations are still debated, therefore this study was done to understand the awareness and knowledge among dental practitioners and students across Saudi Arabia. A cross-sectional and descriptive survey was done on 464 dental practitioners and students, and 21 questions were put forward to assess the knowledge and awareness. All questions were then critically analyzed individually and descriptively concluded with appropriate references. Our study revealed that still very few dental practitioners are aware about the latest advances and use of this technique in pediatric dentistry, and more awareness needs to be created.
Robot Scheduling for Assistance and Guidance in Hospitals
Authors: Siao Cheng-Yan , Chien Ting-Hsuan , Chang Rong-Guey
Published: December 2021

At present, the global COVID-19 epidemic has not slowed down. To reduce the contact between people during the epidemic and prevent the epidemic from expanding, we have developed a robot to assist medical staff in patient guidance and communication services. The robot can provide an emergency contact so that users can immediately contact the counter for help. The user does not have face-face contact with the medical staff. When the robot encounters obstacles in the path of travel, the detected event and the time of occurrence are sent back to the back-end system. It also provides security personnel with real-time images and robot control rights to understand the situation and deal with it in real-time.
Investigation of the Effectiveness of an Augmented Reality and a Dynamic Simulation System Collaboration in Oil Pump Maintenance
Authors: Koteleva Natalia , Valnev Vladislav , Frenkel Ilia
Published: December 2021

The maintenance of oil pumps is a complex task for any operating organization, and for an industrial enterprise in the oil and gas sector of the economy, this issue has a high degree of urgency. One of the reasons for this is a wide spread of pumping equipment in all areas of oil and gas enterprises. At the same time, an aggressive environment, uneven load, remote facilities, and harsh climatic zones (especially in the areas of the Arctic region or production platforms) are factors that make it relevant to develop special systems that help or simplify the maintenance of pumping equipment. Dynamic modeling is one of the modern technologies which allows for solving the urgent issue of assessing the technical condition of equipment. It is the basis of systems that carry out diagnostics and prognostic calculations and allow for assessing the dynamic state of objects under various conditions of their operation, among other functions. Augmented reality technology is a technology that allows for reducing the time for equipment maintenance by reducing the time for searching and processing various information required in the maintenance process. This paper presents an investigation of the effectiveness of an augmented reality and a dynamic simulation system collaboration in oil pump maintenance. Since there is insufficient research on the joint application of these two technologies, the urgent issue is to prove the effectiveness of such collaboration. For this purpose, this paper provides a description of the system structure, gives a description of the development process of the augmented reality system application and tests the application using Microsoft HoloLens 2.
Biospeckle Optical Coherence Tomography (bOCT) in the Speedy Assessment of the Responses of the Seeds of Raphanus sativus L. (Kaiware Daikon) to Acid Mine Drainage (AMD)
Authors: Li Danyang , Rajagopalan Uma Maheswari , De Silva Y. Sanath K. , Liu Fenwu , Kadono Hirofumi
Published: December 2021

The extraction of mineral resources from mines plays a vital role in global socio-economic development. However, acid mine drainage (AMD) has been one of the major pollutants, and a vast area of the agricultural fields has been polluted. Therefore, techniques for monitoring the response of plants to AMD that arise during mineral extraction are necessary. In addition, such a technique becomes especially valuable to understand how the plants could play a role in the phytoremediation of AMD. We propose the use of biospeckle Optical Coherence Tomography (bOCT) to investigate the response of Kaiware daikon seeds under the exposure to simulated AMD at two different concentrations of 40 mL/L and 80 mL/L. OCT images of the Kaiware daikon seed were obtained at a speed of 10 frames per second (1 frame: 512 × 2048 pixels) for a few tens of seconds. For each pixel of the OCT structural images, the contrast across the temporal axis was calculated to give biospeckle contrast OCT images (bOCT images). It was found that bOCT images clearly distinguished the changes due to 40 mL/L and 80 mL/L of AMD treatments from the control within a short time of around an hour, compared to the conventional OCT images that failed to show any changes. This variation was found to be statistically significant and could reflect the internal activity of the seeds. The proposed bOCT method could be a rapid, non-invasive technique for screening suitable plants in AMD phytoremediation applications.
The Combination of Low-Temperature Plasma and Tripterygium wilfordii Hook F on Ameliorating Imiquimod-Induced Psoriasiform Dermatitis in Mice
Authors: Zhang Song , Chen Baihan , Liu Dawei , Chen Hongxiang
Published: December 2021

Improving the transdermal delivery efficiency of medicine is a crucial measure to improve the treatment efficiency of psoriasis. This paper developed a low-cost, highly active, and large-action-area low-temperature plasma (LTP) jet array. The two components of plasma—the high concentration of reactive oxygen and nitrogen species and the strong electric field—easily changed the structural integrity of the stratum corneum, which enhanced the transdermal delivery of the medicine. Tripterygium wilfordii Hook F (TwHF) is a medicine used to treat autoimmune and inflammatory conditions. The enhanced transdermal delivery of TwHF significantly alleviated the severed psoriasiform dermatitis induced by the imiquimod. Unlike the TwHF treatment alone, the LTP + TwHF treatment was more efficient at suppressing epidermal thickening and inhibiting systemic inflammation without noticeable side effects. LTP + TwHF treatment provides a potential new solution for psoriasis treatment.
Evaluation of Lip Morphology and Nasolabial Angle in Non-Syndromic Cleft Lip and/Palate and Non-Cleft Individuals
Authors: Alam Mohammad Khursheed , Alfawzan Ahmed Ali , Akhter Fatema , Alswairki Haytham Jamil , Chaudhari Prabhat Kumar
Published: December 2021

Objective: To investigate the variation between the non-syndromic cleft lip and/or palate (NSCLP) and non-cleft (NC) subjects in relation to the lip morphology (LM) and nasolabial angle (NLA). Materials and Methods: Lateral cephalogram (Late. Ceph.) of 123 individuals (92 NSCLP [29 = bilateral cleft lip and palate (BCLP), 41 = unilateral cleft lip and palate (UCLP), 9 = unilateral cleft lip and alveolus (UCLA), 13 = unilateral cleft lip (UCL)], and 31 NC) who did not undergo any orthodontic treatment were investigated. By WebCeph, an artificial intelligence- (A.I.) driven software, 2 (two) parameters of LM, namely upper lip to E line (LM-1) and lower lip to E line (LM-2), and NLA analysis was carried out for each individual. Multiple tests were carried out for statistical analysis. Results: The mean ± SD observed for LM-1, LM-2, and NLA for NC individuals were 1.56 ± 2.98, 0.49 ± 3.51, and 97.20 ± 16.10, respectively. On the other hand, the mean ± SD of LM-1, LM-2, and NLA for NSCLP individuals were 4.55 ± 4.23, 1.68 ± 2.82, and 82.02 ± 14.66, respectively. No significant variation was observed with respect to gender and side. NSCLP (different types) and NC individuals showed significant disparities in LM-1 and NLA. Conclusion: It can be concluded that parameters of lip morphology such as LM-1, LM-2, and NLA vary among NSCLP and NC individuals.
Hypoxia Modulates Regenerative Potential of Fetal Stem Cells
Authors: Pei Yixuan Amy , Pei Ming
Published: December 2021

Adult mesenchymal stem cells (MSCs) are prone to senescence, which limits the scope of their use in tissue engineering and regeneration and increases the likelihood of post-implantation failure. As a robust alternative cell source, fetal stem cells can prevent an immune reaction and senescence. However, few studies use this cell type. In this study, we sought to characterize fetal cells’ regenerative potential in hypoxic conditions. Specifically, we examined whether hypoxic exposure during the expansion and differentiation phases would affect human fetal nucleus pulposus cell (NPC) and fetal synovium-derived stem cell (SDSC) plasticity and three-lineage differentiation potential. We concluded that fetal NPCs represent the most promising cell source for chondrogenic differentiation, as they are more responsive and display stronger phenotypic stability, particularly when expanded and differentiated in hypoxic conditions. Fetal SDSCs have less potential for chondrogenic differentiation compared to their adult counterpart. This study also indicated that fetal SDSCs exhibit a discrepancy in adipogenic and osteogenic differentiation in response to hypoxia.
Experimental and Numerical Studies of Stress Distribution in an Expanding Pin Joint System
Authors: Salahshour Soheil , Karlsen Øyvind , Lemu Hirpa Gelgele
Published: December 2021

Pin joints are widely used mechanisms in different industrial machineries such as aircrafts, cranes, ships, and offshore drilling equipment providing a joint with possibility of relative rotation about one single axis. The rigidity of the joint and its service lifetime depend on the clamping force in the contact region that is provided by the applied torque. However, due to the tolerance needed for insertion of a pin in the equipment support bore, the pin is prone to relative displacement inside the bore. The amplitude of this relative displacement usually increases as time passes and since the material of the support often has lower quality grade than the pin, it leads to creation of slack in the equipment and malfunctioning of the machine. An Expanding Pin System (EPS) can be a solution to this problem where the split sleeve expands to remove the gap while the joint is torqued. Therefore, slack in the joint system disappears and 360° contact area could be achieved, providing a better stress distribution and preventing the stress localization. Determining the EPS preload and the resulting contact pressure and stresses in the joint parts are important to avoid damaging to the contact surfaces of the joints and making the dismantling of the EPS difficult. Therefore, finding the amount of the required torque is a compromise between preventing slack in the EPS and prohibiting damage to the joint parts. Stress analysis in this study is performed based on the industrially recommended torque for the EPS type under study. This article reports the study conducted on the stress distribution and the magnitude of stresses exerted to the equipment support when EPS is installed on the machine. To achieve this purpose and to investigate the stress distribution in the joint, both experimental and finite element (FE) methods were used. The experimental results show how much of the applied energy to the EPS in the form of torque is spent to expand the split sleeve and test boss and also to overcome friction. The finite element analysis provides magnitude and distribution of stresses in the EPS components.
Sculpture in Socialist Realism—Soviet Patterns and the Polish Reality
Authors: Tomaszewicz Agnieszka
Published: December 2021

Socialist realism was more than just a trend in art. It was also, and perhaps predominantly, a method of educating the new post-revolutionary society in the Union of Soviet Socialist Republics. In socialism, the state became the commissioner, consumer, and critic of art, treating it as a major propaganda tool. It is thus not surprising that the socialist realism patterns were imposed on artists working in those countries which found themselves in the Soviet sphere of influence after the end of the Second World War. In Poland, which was the Soviet Union’s closest neighbour and one of the larger countries in the post-war “Eastern Bloc”, socialist realism was the only permitted creative method in the years 1949–1956. The ideologists of the new art assigned a special role to sculpture, which, next to posters and murals, was considered the most socially accessible form of artistic expression due to the possibility of placing it in public space. Monuments as material carriers of ideology were used as an expression of power, but they also marked the places of strengthening collective identity. During the period of socialist realism in Poland, sculptural activity followed the main three directions: heroic, portrait, and architectural–decorative. Therefore, this paper aims to present theoretical and ideological assumptions relating to socialist sculpture and their confrontation with realisations in Poland during the period of the Soviet artistic doctrine. The paper also presents the aesthetic paradigms of socialist sculptures and their relationships with the canons of European art, and, for Poland, also with the native art, mainly sacral.
The Times of Caring in a Nuclear World: Sculpture, Contamination and Stillness
Authors: Heim Wallace
Published: December 2021

Care takes time. Caring, whether with, for, or about a living being or entity that is more-than-human, disrupts expectations of how a linear, human time should progress. To practice care for the contaminated, the lands, waters, and animate life altered by human industry, is to extend that indeterminacy into distant, deeper time. Aesthetic representation of the affective and ethical dimensions of care, in this extreme, offers an experience that can transfer the arguments about nuclear contamination into more nuanced and sensed responses and contributes to current thinking about care in the arts worlds. I was commissioned to make a sculpture exhibition in 2020 as part of an anthropological study into the future of the Sellafield nuclear site in West Cumbria, UK. The exhibition, ‘x = 2140. In the coming 120 years, how can humans decide to dismantle, remember and repair the lands called Sellafield?’, consisted of three sculptural ‘fonts’ which engaged with ideas of knowledge production, nuclear technologies, and the affective dimensions of care about/for/with the contaminated lands and waters. This article presents my intentions for the sculptures in their context of a nuclear-dependent locale: to engage with the experience of nuclear futures without adversarial positioning; to explore the agential qualities of the more-than-human; and to create a stillness expressive of the relationality of the human and the contaminated through which one could fathom what care might feel like. These intentions are alongside theories of time, aesthetics, and care across disciplines: care and relational ethics, science and technology studies, and nuclear culture.
3D Printing and Implementation of Digital Twins: Current Trends and Limitations
Authors: Kantaros Antreas , Piromalis Dimitrios , Tsaramirsis Georgios , Papageorgas Panagiotis , Tamimi Hatem
Published: December 2021

Fabricating objects with desired mechanical properties by utilizing 3D printing methods can be expensive and time-consuming, especially when based only on a trial-and-error test modus operandi. Digital twins (DT) can be proposed as a solution to understand, analyze and improve the fabricated item, service system or production line. However, the development of relevant DTs is still hampered by a number of factors, such as a lack of full understanding of the concept of DTs, their context and method of development. In addition, the connection between existing conventional systems and their data is under development. This work aims to summarize and review the current trends and limitations in DTs for additive manufacturing, in order to provide more insights for further research on DT systems.
Relationship between Wildfire Smoke and Children’s Respiratory Health in the Metropolitan Cities of Central-Chile
Authors: Ciciretti Rebecca , Barraza Francisco , De la Barrera Francisco , Urquieta Lorna , Cortes Sandra
Published: December 2021

Wildfire causes multiple problems for people living in cities. One of them is the deterioration of air quality as a result of wildfire smoke. This smoke can consequently have effects on human health. The present study aims to characterize the relationship between the occurrence of wildfires in central Chile and the effects on children’s respiratory health. Public databases provided the number of emergency care visits, wildfires, and concentration of air pollutants, demographics and meteorological variables for the regions of Santiago and Valparaiso from 2010 to 2013. Time series analysis was used monthly on health care visits to determine the relative health risk in children when in the presence of additional wildfires. Significant health risks were observed in Santiago for children younger than 1-year-old of bronchitis (RR 1.007, CI 95% 1.007–1.008; chronic lower respiratory diseases (RR 1.012, CI 95% 1.012–1.013); and pneumonia (RR 1.026 CI 95% 1.026–1.027) and in children aged one to four years old (RR 1.016 CI 95% 1.015–1.016). A dose-response relationship was also observed for pneumonia, showing that it affects younger children particularly when there is an increase in the number of wildfires. In the Region of Valparaíso, wildfires did not significantly change the risk of respiratory illness, this could be due to favorable ventilation. Currently, Santiago has an urgent need for monitoring and the evaluation of the damage to children’s respiratory health, along with the development of comprehensive prevention strategies.