bluuebunny/crossref_metadata_2025 huggingface dataset:
Type of Tea Consumption and Depressive Symptoms in Older Adults
Authors: Yao Yao , Chen Huashuai , Chen Lele , Ju Sang-Yhun , Yang Huazhen , Zeng Yi , Gu Danan , NG Tze Pin
Published: November 2020

BackgroundExisting research indicates that tea drinking may exert beneficiary effects on mental health. However, associations between different types of tea intake and mental health such as depression are not fully examined. The purpose of this study was to examine the associations of green tea, fermented tea, and flowering tea consumption with depressive symptoms.MethodsWe used data from the 2018 wave of Chinese Longitudinal Healthy Longevity Survey. The type (green, fermented [black, Oolong, white, yellow, dark, and compressed teas], and flower) and frequency of tea consumption and depressive symptoms for 13,115 participants were assessed. We examined the associations between type and frequency of tea intake and depression, controlling for a set of demographic, socioeconomic, psychosocial, behavioral, and health-related variables. ResultsOverall, intake of green tea, fermented tea, and flower tea were all significantly associated with a lower prevalence of depression, independent of other risk factors. Compared with the group for no tea intake, the adjusted ORs for daily green tea, fermented tea, and flower tea intake were 0.85 (0.76-0.95), 0.87 (0.76-0.99), and 0.70 (0.59-0.82), respectively. Linear associations were observed between frequency of all types of tea intake with depressive symptoms (Ps for trend<0.05). The associations of type and frequency of tea intake and depressive symptoms were robust in several sensitivity analyses.ConclusionsAmong Chinese old adults, regularly consumed any type of tea (green, fermented or flower) were less likely to show depressive symptoms, the associations seemed more pronounced among flower tea and green tea drinkers.
Exome Sequencing Revealed PDE11A as a Novel Candidate Gene for Early-Onset Alzheimer's disease
Authors: Qin Wei , Zhou Aihong , Zuo Xiumei , Jia Longfei , Wei Cuibai , Tang Yi , Wang Fen , Li Fangyu , Wang Qi , Li Ying , Wei Yiping , Jin Hongmei , Cruchaga Carlos , Benitez Bruno A. , Jia Jianping
Published: November 2020

Background: Alzheimer’s disease (AD) is a leading cause of dementia in the elderly and has become a major health issue. However, a large number of genetic risk factors remain undiscovered. Methods: To identify novel risk genes and better understand the molecular pathway underlying AD, whole-exome sequencing (WES) was performed in 215 early-onset AD (EOAD) patients and 55 unrelated healthy controls of Han Chinese ethnicity. Subsequent direct sequencing was performed in 4962 individuals to validate the selected rare mutations. Computational annotation and in vitro functional studies were performed to evaluate the role of candidate mutations in EOAD and the underlying mechanisms.Results: We identified two rare missense mutations in the phosphodiesterase 11A (PDE11A) gene, resulting in p.Arg202His, and p.Leu756Gln, in individuals with EOAD. Both mutations are located in evolutionarily highly conserved amino acids, are predicted to alter the protein conformation, and classified as pathogenic. Furthermore, we found significantly decreased protein levels of PDE11A in brain samples of AD patients. Expression of PDE11A variants and knockdown experiments with specific short hairpin RNA (shRNA) for PDE11A both resulted in an increase of AD-associated Tau hyperphosphorylation at T181, S404, S202, S416, S214, S396 and AT8 epitopes in vitro. PDE11A variants or PDE11A shRNA also caused increased cAMP levels, protein kinase A (PKA) activation, and cAMP response element-binding protein (CREB) phosphorylation. Additionally, pretreatment with a PKA inhibitor (H89) suppressed PDE11A mutation-induced p-Tau formation.Conclusions: Our results demonstrate that both PDE11A mutations and PDE11A knockdown increase Tau phosphorylation through the cAMP/PKA pathway, suggesting that PDE11A is a novel risk gene for AD. This study provides insight into the involvement of Tau phosphorylation via the cAMP/PKA pathway in EOAD pathogenesis and provides a potential new target for intervention.
Gut Flora-Targeted Photobiomodulation Therapy Improves Senile Dementia in an Aß-Induced Alzheimer’s Disease Animal Model
Authors: Chen Qianqian , Wu Jinpeng , Yin Huijuan , Dong Xiaoxi , Shi Xiafei , Su Siying , Che Boshen , Li Yingxin
Published: November 2020

Background: Emerging evidence suggests that the gut microbiota plays an important role in the pathological progression of Alzheimer’s disease (AD). Photobiomodulation (PBM) therapy is believed to have a positive regulatory effect on the imbalance of certain body functions, including inflammation, immunity, wound healing, nerve repair, and pain. Previous studies have found that the intestinal flora of patients with AD is in an unbalanced state. Therefore, we have proposed the use of gut flora-targeted PBM (gf-targeted PBM) as a method to improve AD in an Aß-induced AD mouse model. Methods: PBM was performed on the abdomen of the mice at the wavelengths of 630 nm, 730 nm, and 850 nm at 100 J/cm2 for 8 weeks. Morris water maze test, immunofluorescence and proteomic of hippocampus, and intestinal flora detection of fecal were used to evaluate the treatment effects of gf-targeted PBM on AD rats.Results: PBM at all three wavelengths (especially 630 nm and 730 nm) significantly improved learning retention as measured by the Morris water maze. In addition, we found reduced amyloidosis and tau phosphorylation in the hippocampus by immunofluorescence in AD mice. By using a quantitative proteomic analysis of the hippocampus, we found that gf-targeted PBM significantly altered the expression levels of 509 proteins (the same differentially expressed proteins in all three wavelengths of PBM), which involved the pathways of hormone synthesis, phagocytosis, and metabolism. The 16s rRNA gene sequencing of fecal contents showed that PBM significantly altered the diversity and abundance of intestinal flora. Specifically, PBM treatment reversed the typical increase of Helicobacter and uncultured Bacteroidales and the decrease of Rikenella seen in AD mice. Conclusions: Our data indicate that gf-targeted PBM regulates the diversity of intestinal flora, which may improve damage caused by AD. Gf-targeted PBM has the potential to be a noninvasive microflora regulation method for AD patients.
Family With Sequence Similarity 83, Member A (FAM83A) As a Promising Prognostic Biomarker in Lung Squamous Cell Cancer
Authors: Wang Cong , Lu Longfei , Feng Zitong , Li Yongmeng , Lu Ming , Tian Hui
Published: November 2020

Background: Increasing studies have found the dysregulated FAM83A as a potential biomarker in various cancers. Its function in cancer cells is largely unknown, and especially their role in LUSC remains unclear. We detected the expression level and prognosis role of FAM83A in LUSC.Methods: The bioinformatics methods were performed initially to predict the expression level and prognostic value of FAM83A mRNA in LUSC. We used IHC to examine its protein expression level and identify its prognostic value using 132 pairs of tissues.Results: Results from TCGA and Oncomine databases revealed that FAM83A mRNA expression level was significantly higher in LUSC than that in normal lung tissue. TCGA and GEO databases revealed FAM83A mRNA overexpression was significantly associated with poorer OS of LUSC patients (all P<0.05). Furtherly, our IHC results revealed that FAM83A was overexpressed in 78 (59.1%) patients and 19(14.4%) pancancerous tissues (P<0.001). Kaplan-Meier analyses revealed that high FAM83A protein expression was significantly associated with decreased 5-years’ OS (P = 0.007) and PFS (P = 0.007). Via multivariate analysis, FAM83A expression level was independent prognostic factor for both OS (P = 0.006) and PFS (P = 0.002).Conclusion: FAM83A was overexpressed in LUSC and it could serve as a prognosis prediction biomarker for LUSC. FAM83A could act as one new potential therapeutic target for LUSC treatment.
Cancer-Associated Fibroblasts Promote the Survival of Irradiated Nasopharyngeal Carcinoma Cells via the NF-κB Pathway
Authors: Weiqiang Huang , Longshan Zhang , Mi Yang , Xixix Wu , Xiaoqing Wang , Wenqi Huang , Lu Yuan , Hua Pan , Yin Wang , Zici Wang , Yuting Wu , Jihong Huang , Huazhen Liang , Shaoqun Li , Liwei Liao , Laiyu Liu , Jian Guan
Published: November 2020

Background: Irradiation has emerged as a valid tool for nasopharyngeal carcinoma (NPC) in situ treatment; however, NPC derived from tissues treated with irradiation is a main cause cancer-related death. The purpose of this study is to uncover the underlying mechanism regarding tumor growth after irradiation and provided potential therapeutic strategy. Methods: Fibroblasts were extracted from fresh NPC tissue and normal nasopharyngeal mucosa. Immunohistochemistry was conducted to measure the expression of α-SMA. Cytokines were detected by protein array chip and identified by real-time PCR. CCK-8 assay was used to detect cell proliferation. Radiation-resistant (IRR) 5-8F cell line was established and colony assay was performed to evaluate tumor cell growth after irradiation. Signaling pathways were acquired via gene set enrichment analysis (GSEA). Comet assay was used to measure DNA damage level. Protein expression was detected by western blot assay. Results: We found that radiation-resistant NPC tissues were constantly infiltrated with a greater number of cancer-associated fibroblasts (CAFs) compared to radiosensitive NPC tissues. Further research revealed that CAFs induced the formation of radioresistance and promoted NPC cell survival following irradiation via the IL-8/NF-κB pathway to reduce irradiation-induced DNA damage. Treatment with Tranilast, a CAF inhibitor, restricted the survival of CAF-induced NPC cells and attenuated the of radioresistance properties. Conclusions: Together, these data demonstrate that CAFs can promote the survival of irradiated NPC cells via the NF-κB pathway and induce radioresistance that can be interrupted by Tranilast, suggesting the potential value of Tranilast in sensitizing NPC cells to irradiation.
Dietary Supplementation With Creatine Pyruvate Alter Rumen Microbiota Protein Function in Heat-Stressed Beef Cattle
Authors: Li Yanjiao , Zhao Xianghui , Liu Lin , Mao Kang , Qu Mingren , Lu Guwei , Zang Yitian
Published: November 2020

Background: Creatine pyruvate (CrPyr) is a new multifunctional nutrient that can provide both pyruvate and creatine. It has been shown to relieve the heat stress of beef cattle by improving antioxidant activity and rumen microbial protein synthesis, but the mechanism of CrPyr influencing rumen fermentation remains unclear. This study aimed to combine 16S rDNA sequencing and metaproteomics technologies to investigate the microbial composition and function in rumen fluid samples taken from heat-stressed beef cattle treated with or without 60 g/d CrPyr. Results: 16S rDNA sequencing revealed that there was no significant differences in the α-diversity indices between the two groups. By analyzing the expression profiles of 700 distinct proteins, we found that CrPyr administration increased the fatty acid β-oxidation, promoted the interconversion from pyruvate to phosphoenolpyruvate, acetyl-CoA and malate, up-regulated gluconeogenesis and citrate cycle metabolism, and promoted the biosynthesis of amino acids. Conclusions: The increased generation of ATP during fatty acid β-oxidation or citrate cycle and the up-regulation synthesis of microbial protein in rumen of beef cattle treated with CrPyr, may help decreased oxidative stress, regulate energy metabolism, and further improve the rumen fermentation characteristic under heat stress.
New mechanistic insight into the digestion of complex dietary fibre by rumen microbiota using combinatorial high-resolution glycomic and transcriptomic analyses
Authors: Badhan Ajay , Low Kristin , Jones Darryl , Xing Xiaohui , Milani Mohammad Raza Marami , OrtegoPolo Rodrigo , Abbott D. Wade , Venketachalam Sivasankari , Hahn Michael , McAllister Tim
Published: November 2020

Background
The rumen microbial community is considered the most efficient anaerobic digestive ecosystem known, yet less than half of the energy in low quality forages is actually metabolized. There is a knowledge gap regarding the specific factors that impede the ruminal digestion of plant cell walls or if rumen microbiota have the functional potential and activities to overcome these constraints. To address these issues, innovative experimental methods may provide a high-resolution understanding of the cell wall chemistries and higher-order structures that are resistant to microbial digestion and how they interact with the functional activities of the rumen microbial community.
Results
With this goal, we characterized the total tract indigestible residue (TTIR) from cattle fed a high-forage diet containing low-quality straw using two comparative glycomic approaches: ELISA-based glycome profiling and glycosidic linkage analysis. Using these techniques, we successfully detected numerous and diverse cell wall glycan epitopes in barley straw and TTIR and determined their relative abundance pre- and post-intestinal digestion. Of these, xyloglucans and heteroxylans were the most recalcitrant to digestion. Linkage analysis identified indigestible linkages consistent with the polysaccharide epitopes identified by ELISA-based glycome analysis. To determine if residual plant polysaccharides within TTIR could be metabolised, rumen microbiota from cannulated cattle fed barley straw were incubated with barley straw and TTIR in in vitro batch cultures. Transcript coding for carbohydrate-active enzymes (CAZymes) were identified and characterized for their contribution to cell wall digestion based on glycomic analyses, comparative gene expression profiles, and associated CAZyme families. High-resolution phylogenetic fingerprinting of these sequences revealed encoded enzymes with activities predicted to cleave the primary linkages within heteroxylan and arabinan.
Conclusion
This experimental platform provides unprecedented precision in the understanding of forage structure and digestibility, which can inform next-generation solutions to improve the growth of ruminants fed low quality forages and enhance the use of crop residues as a feedstock.
Factors associated with self-rated health among mineworkers in Zambia: a cross-sectional study
Authors: Ohnishi Mayumi , Tembo Backsion , Nakao Rieko , Matsuura Emi , Fujita Wakako
Published: November 2020

Background
This study aims to examine miners’ working conditions and self-rated health status in copper mines in Zambia and to identify conditions and factors necessary to improve the safety and health of mineworkers.
Methods
A self-administered questionnaire was conducted anonymously among copper mineworkers in Zambia in 2015 and 2016.
Results
In total, 285 of 330 responses were analyzed. Regular employees had better working conditions, including higher incomes (t-test, P = 0.011), guaranteed paid holidays (chi-square test, P = 0.045), health check-ups (chi-square test, P = 0.023) and sickness insurance (chi-square test, P < 0.001), than contract employees. Mineworkers’ increased self-rated health was determined by an educational status of having completed secondary school (adjusted odds ratio [AOR]: 3.406; 95% confidence interval [CI]: 1.072, 10.822; P = 0.038). Moreover, job category, such as working underground (AOR: 0.338; 95% CI: 0.173, 0.663; P = 0.002), and having experienced violence (AOR: 0.472; 95% CI: 0.035, 0.948; P = 0.035) were negatively associated with good self-rated health in the logistic regression analysis, regardless of employment status.
Conclusions
Among mineworkers in Zambia, non-underground work and not having experienced violence from their boss contributed to increased self-rated health. From the perspective of psychological safety and human security, the management of safety and the working environment, including human resource management and preventing harassment/violence, should be assured, especially for underground mineworkers.
Conceptual Hybrid Model for Wearable Cardiac Monitoring System
Authors: Ahmed Hafiz Imtiaz , Ahmed Hafiz Imtiaz , Saleem Darakhshan Mehboob , Saleem Darakhshan Mehboob , Omair Syed Muhammad , Omair Syed Muhammad , Shams Sarmad , Shams Sarmad , Sheikh Naeem , Sheikh Naeem , Tariq Areeba , Tariq Areeba
Published: November 2020

BackgroundThe electrocardiogram is the most convenient and widely used method of cardiac monitoring. The information provided by the ECG has the potential to be used as a means by which cardiac arrhythmia can be detected at an early stage in order to prevent life-threatening complications. Its significance is widely accepted in the medical field so much so that tele-monitoring is being utilized across the world for cardiac activity. To perform cardiac monitoring more efficiently, a mobile application, used in conjunction with a sensor unit, is designed to perform real-time monitoring of the cardiac signal. The device consists of 3-lead EKG patches with an integrated Bluetooth module allowing a point-to-point pairing between the hardware and smartphone application. The hardware can either be placed on humanoid robot arm fingers or connected to a wearable patch placed on the chest. A real-time EKG signal is transmitted to the Android application on which a time vs. voltage plot will be displayed. ResultsThe device was tested using the ProSim8 ECG simulator by Fluke Biomedical. The test confirmed the signal quality of the ECG signal with clear P, QRS, and T waves. ConclusionsThis device provides a more cost-effective telemedicine solution for cardiac home care assistance in remote areas which can serve as a viable alternative to conventional monitors as it has the potential to reduce the time for clinical procedures
Rabbit Thyroid Extracellular Matrix as a 3D Bioscaffold for Thyroid Bioengineering
Authors: Weng Jie , Chen Bi , Xie Mengying , Wan Xinlong , Peng Wang , Zhou Xiaoming , Zhou Zhiliang , Mei Jin , Wang Liang , Huang Duping , Chen Chan , Wang Zhibin , Wang Zhiyi
Published: November 2020

BackgroundAdvances in regenerative medicine technologies have been strongly proposed in the management of thyroid diseases. Mechanistically, the adoption of thyroid bioengineering requires a scaffold that shares a similar three dimensional (3D) space structure, biomechanical properties, protein component, and cytokines to the native extracellular matrix (ECM).Methods24 male New Zealand white rabbits were used in this experimental study. The rabbit thyroid glands were decellularized by immersion/agitation decellularization protocol. The 3D thyroid decellularization scaffolds were tested with histological and immunostaining analyses, scanning electron microscopy, DNA quantification, mechanical properties test, cytokine assay and cytotoxicity assays. Meanwhile, the decellularization scaffold were seeded with human thyroid follicular cells, cell proliferation and thyroid peroxidase were determined.ResultsNotably, through the imaging studies, it was distinctly evident that our protocol intervention minimized cellular materials and maintained the 3D spatial structure, biomechanical properties, ECM composition, and biologic cytokine. Consequently, the decellularization scaffold was seeded with human thyroid follicular cells, thus strongly revealing its potential in reinforcing cell adhesion, proliferation, and preserve important protein expression.Conclusions﻿The adoption of our protocol to generate a decellularized thyroid scaffold can potentially be utilized in transplantation to manage thyroid diseases through thyroid bioengineering.
Finite Element Analysis of Elbow Joint Stability by Different Flexion Angles of the Annular Ligament
Authors: Xu Guangming , Yang ZhengZhong , Yang JiYong , Liang Ziyang , Li wei
Published: November 2020

ObjectiveTo investigate the biomechanical effects of different flexion angles of the annular ligament on elbow joint stability. MethodsLeft elbow CT and MRI scans were chosen from a healthy volunteer, according to a previous research model. A cartilage and ligament model was constructed with SolidWorks software according to the MRI results to simulate the annular ligament during normal, loosen, and rupture conditions at different buckling angles (0, 30, 60, 90, 120). In 15 elbow models, boundary conditions were set according to the literature. The different elbow 3D finite element models were imported into ABAQUS software to calculate and analyze the load, contact area, contact stress and stress of the medial collateral ligament of the olecranon cartilage. Results1. According to the analysis results, olecranon cartilage stress values when the annular ligament under different conditions（normal、loosened、ruptured）with elbow extension, were 2.1 ± 0.18, 2.4 ± 0.75, and 2.9 ± 0.94 MPa. As the buckling angle increased, the stress value decreased; with 120 degrees of elbow flexion, the minimum stress values were 0.9 ± 0.12, 1.1 ± 0.38, and 1.2 ± 0.29 MPa. 2. When the contact surface of the olecranon cartilage was flexed from 0 to 30 degrees, the olecranon cartilage contact area significantly increased, reaching a maximum value of 254±5.35 mm, and then the contact area gradually decreased, reaching a minimum value of 176±2.62 mm when the elbow joint was flexed to 120 degrees. The results when the annular ligament was loosened and ruptured were different from those of the normal annular ligament. The maximum values were 283±4.74and 312±5.49mm at 60 degrees of elbow flexion. The contact area gradually decreased with an increase in the angle, and the minimum values were 210±3.82 and 236±6.59 mm at 120 degrees of elbow flexion. 3. When the elbow joint was extended, the maximum stress of the medial collateral ligament was 6.5±0.23, 11.5±0.78 and 18.7±0.94 MPa under different states; as the stress decreased with an increase in the angle, the corresponding values were 2.8±0.18, 4.8±0.56 and 6.2±0.72 MPa at 120 degrees of elbow flexion. ConclusionThe annular ligament plays an important role in maintaining elbow joint stability. When the annular ligament ruptures, it should be reconstructed as much as possible to avoid the elevation of stress on the surface of the medial collateral ligament of the elbow and on the annular cartilage, which may cause clinical symptoms.
WITHDRAWN: The Dosimetric Advantages of Robust Optimization Combined With Flatten Filter Free Mode in Left Breast Cancer
Authors: Luo Hongying , Shan Guoping , Shao Kainan , Yang Yiwei , Zhou Xia , Zhou Jianliang
Published: November 2020

Background and purposesTo explore the advantages of Robust optimization in dosimetry under flatten filter free(FFF) Mode after left breast cancer surgery.Materials and methods21 patients with left breast cancer after surgery were randomly selected from 2019 to 2020. The planned target volume (PTV) dose was prescribed 50Gy /25times and B. With or without Robust optimization were designed on the RayStation planning system with Volume rotary intensity-modulated radiotherapy technology based on the FFF Mode. By moving the center point of the field to simulated the movement of target area of the internal chest wall (0.50cm) caused by Respiratory movement, dosimetry characteristics. Using spss 23.0 to analyse the datum.Resultswhen the chest wall target moved outward, the PTV target area D98, D95, D2, CI and HI with Robust optimization were better than those without Robust, and the coverage rate of PTV-CHESTV50 was significantly higher than that without Robust optimization (P=0.000060), which was 15.39% higher than that without Robust optimization. CTVV50 coverage with Robust optimization was higher than that without Robust optimization, with an increase of 14.48%. In terms of endangering organ parameters, the average spinal cord dose of the plan with Robust optimization was 13.19% lower than that of the plan without Robust optimization, and the lung V5 of the plan without Robust optimization was slightly lower than that of the plan with Robust optimization, which was 1.80% lower than that of the plan without Robust optimization. There was no significant difference in machine execution efficiency between the two groups (P > 0.05).ConclusionsThe Robust optimization could be adopted in the development of postoperative left breast cancer radiotherapy plan, which ensures that the target dose coverage and the dose limit of organ-at-risk still meet the clinical requirements under condition of chest wall displacement caused by respiratory movement.
Sugammadex is associated with shorter hospital length of stay after open lobectomy for lung cancer: A retrospective observational study
Authors: Song Seung Won , Yoo Kyung Yeon , Ro Yong Sung , Pyeon Taehee , Bae Hong-Beom , Kim Joungmin
Published: November 2020

Background: Sugammadex is associated with few postoperative complications. Postoperative pulmonary complications are related to prolonged hospitalizations. Present study explored whether the use of sugammadex could reduce postoperative complications and thereby reduce hospital length of stay (LOS) after lung surgery. Methods: We reviewed the medical records of patients who underwent elective open lobectomy for lung cancer from January 2010 to December 2015. Patients were divided into the sugammadex group and pyridostigmine group.The primary outcome was hospital LOS and secondary outcomes were postoperative complications and overall survival at 1 year. The cohort was subdivided into patients with and without prolonged LOS to explore the effects of sugammadex on outcomes in each group. Risk factors for prolonged LOS were determined via multivariate analyses. After propensity score matching, 127 patients were assigned to each group. Results: Median hospital LOS was shorter (10.0 vs. 12.0 days) and the incidence of postoperative atelectasis was lower (18.1 vs. 29.9%) in the sugammadex group. However, no significant difference in overall survival between the groups was seen over 1 year (hazard ratio, 0.967; 95% confidence interval, 0.363 to 2.577). Sugammadex was a predictor related to LOS (exponential coefficient 0.88; 95% CI 0.82–0.95) and resulted in a shorter LOS in patients without a prolonged LOS). Among patients undergoing open lung lobectomy for lung cancer, compared to pyridostigmine, neuromuscular reversal with sugammadex resulted in a short hospital LOS and a lower incidence of postoperative complications, but showed a similar mortality. Conclusions: Our data suggest that sugammadex is a preferable agent for neuromuscular blockade (NMB) reversal than cholinesterase inhibitors in this patient population.Trial registration: This study registered in the Clinical Research Information Service of the Korea National Institute of Health (approval number: KCT0004735, Date of registration: 21 January 2020, Retrospectively registered).
Comparison of Physical Effect Between Two Training Methods for Individuals With Substance Use Disorder
Authors: yang yanguang , Chen Jing-yi , Pang Xiao-wu , Shen Meng-lu , Yang Su-yong , Xu Ding , Xiao Ke , Wang Tian-yuan , Wang Jia-bin , Zhu Dong
Published: November 2020

Background: High-intensity Interval Training (HIIT) has recently been widely used for health promotion in healthy people and patients with chronic diseases. It is not clear whether HIIT can bring better physical rehabilitation benefits to individuals with substance use disorder (SUD) than that of Moderate-intensity continuous Training (MICT). The study aimed to compare the effects of HIIT versus MICT on the physical fitness of individuals with SUD.Methods: A total 120 individuals with amphetamine-type stimulant dependent from a compulsory drug rehabilitation center voluntarily took part in this study. They were randomly assigned to the HIIT group (experimental group) and MICT group (control group). The subjects in experimental group received HIIT training three times a week (80%-85%HRMax, 60min). The exercise included rope jumping, running, weight training and basketball game. The subjects from control group received MICT 3 times a week (60%HRMax, 60min), including Tai Chi, mind-body exercise and recreation activity. Physical fitness assessment were performed at the baseline, 3 months, 6 months, 9 months and 12 months exercise intervention including blood pressure (BP), vital capacity (VC), hand grip, push-up, sit-and-reach, one-leg-stand with eyes closed and choice reaction time. The craving level was assessed using the visual analog scale （VAS）at the baseline, 6 months and 12 months. A two-way repeated measures analysis of variance (ANOVA) was applied to test whether the intervention were different after 12 months.Results: The within-group factor displayed significant changes in HIIT and MICT groups in terms of the systolic BP (F (4,336) =12.799), diastolic BP (F(4,336) =9.495), VC (F(4,336)=18.121), hand grip (F(4,336)=34.815), sit-and-reach (F(4,336) =13.871), choice reaction time (F(4,336) =20.603), one-leg-stand with eyes closed (F(4,336) =14.495) and push-up (F(4,336) =28.805). The craving level decreased after 12 months intervention in both groups (F(2,168) =11.25, P<0.001), but there was not significant differences between HIIT and MICT group.Conclusion: Both HIIT and MICT can promote the physical fitness rehabilitation for individuals with SUD. Clinical Trials Registry: ChiCTR1900022158 Chinese Clinical Trial Registry
A Case of Esophageal Cancer With Right Vertebral Artery Variation Observed During Thoracoscopic Esophagectomy
Authors: Sato Yuta , Tanaka Yoshihiro , Imai Takeharu , Hatanaka Yuji , Okumura Naoki , Matsuhashi Nobuhisa , Takahashi Takao , Yoshida Kazuhiro
Published: November 2020

BackgroundVariation of the vertebral artery bifurcation is rare. This branching abnormality can make it difficult to identify the recurrent laryngeal nerve (RLN) during thoracoscopic esophagectomy. There are a few reports on abnormal branching of the vertebral artery but none related to esophagectomy. We report the case together with the results of the evaluation of vertebral artery bifurcation and length in 50 patients with esophageal cancer in our hospital.Case presentationThoracoscopic esophagectomy was performed on a 70-year-old patient with esophageal cancer. During lymph node dissection, an unusual blood vessel was found running along the right subclavian artery (RSA). This blood vessel made it difficult to identify the right RLN. We determined this blood vessel to be the right vertebral artery (RVA) branching far more centrally than usual. Because this anatomical abnormality was clarified, we could then recognize that the right RLN coursed around the RVA and the RSA and thus was running in a larger arch than usual.Conclusion﻿Knowledge of such anatomical variation is important in thoracoscopic esophagectomy to prevent iatrogenic injury of the RLN and the vertebral artery.
The Short-term and Long-term Outcomes of Indocyanine Green Tracer-Guided Laparoscopic Radical Gastrectomy in Patients with Gastric Cancer
Authors: Lu Xiaofeng , Liu Song , Xia Xuefeng , Sun Feng , Liu Zhijian , Wang Jiafeng , Li Xianghui , Yang Zhengyang , Kang Xing , Ai Shichao , Guan Wenxian
Published: November 2020

Background: the safety and efficacy of indocyanine green (ICG) imaging navigational laparoscopic gastrectomy remain controversial. This study is to evaluate the short-term and long-term outcomes of ICG-guided laparoscopic radial gastrectomy in patients with gastric cancer.Methods: Consecutive patients with definitive diagnosis of gastric cancer that underwent laparoscopic radical gastrectomy were collected retrospectively. Propensity score matching (PSM) at 1:1 ratio was performed to compare the outcomes of two groups.Results: A total of 122 qualified patients were divided into ICG group (n=34) and non-ICG group (n=88). PSM yielded 28 patients with comparable baseline characteristics into each group. The number of retrieved lymph node in ICG group was significantly higher than that in non-ICG group (P=0.0196). There was no statistical difference of perioperative, short-term and long-term complications between two groups. Conclusion: ICG-guided laparoscopic radical gastrectomy is safe and effective, and improves the number of retrieved lymph nodes for patients with gastric cancer.
Short-term Outcomes of Refractory Diabetic Macular Edema Switch from Ranibizumab to Dexamethasone Implant and the Influential Factors: A Retrospective Real World Experience.
Authors: Hsia Ning-Yi , Lin Chun-Ju , Chen Huan-Sheng , Chang Cheng-Hsien , Bair Henry , Lai Chun-Ting , Lin Jane-Ming , Chen Wen-Lu , Tien Peng-Tai , Wu Wen-Chuan , Tsai Yi-Yu
Published: November 2020

Background: To evaluate the effectiveness and safety of intravitreal dexamethasone (DEX) implants in refractory diabetic macular edema (DME) treated by intravitreal ranibizumab.Methods: We retrospectively analyzed DME patients who received DEX implant treatment after being unresponsive to at least three monthly intravitreal ranibizumab injections. The main outcomes were best-corrected visual acuity (BCVA), central retinal thickness (CRT), and intraocular pressure (IOP).Results: Twenty-nine eyes of 26 patients who had previously received an average of 8.1 ± 4.4 ranibizumab injections were included. Patients received between one and three DEX implants during 12.4 ± 7.4 months of follow-up. The mean final CRT significantly decreased from 384.4 ± 114.4 μm at baseline to 323.9 ± 77.7 μm (p = 0.0249). The mean final BCVA was 51.4 ± 21.3 letters, which was not significant compared to baseline (44.9 ± 30.2 letters, p = 0.1149). Mean IOP did not increase significantly. All patients tolerated the treatment well without serious adverse events. Higher baseline CRT and worse BCVA correlated with better therapeutic responses.Conclusion: Switching to DEX implant is effective and safe for treating DME non-responder to intravitreal ranibizumab in real world.
Integrated Transcriptome, Proteome, Acetylome, and Metabolome Profiling of Mouse Liver During Normal Aging
Authors: Liu Jiang-Feng , Wu Song-Feng , Liu Cong , Chen Hou-Zao , Yang Juntao
Published: November 2020

BackgroundAging is a complex biological process accompanied by a time-dependent functional decline that affects most living organisms. We aimed to obtain an integrated aging-associated profile of the mouse liver using a multi-omics approach.ResultsWe performed a combined transcriptome, proteome, acetylome, and metabolome analysis of liver tissues from young and old mice under physiological conditions. Old mice were frequently obese with a fatty liver, and the observed profile changes in different omics were generally moderate. Specifically, transcriptome, proteome, and acetylome analyses revealed different patterns in old and young mice, but metabolome analysis did not. Functional enrichment analysis showed that metabolic pathways were broadly altered during normal aging. Notably, the genes, proteins, and metabolites involved in pyrimidine and glutathione metabolisms were significantly affected in all these four omics. Moreover, we observed increased arachidonic acid metabolism and decreased complement and coagulation cascades in old mice, suggesting an alteration in the immune function during normal aging.ConclusionsWe conducted a multi-omics investigation of normal liver aging in mice and generated comprehensive datasets for aging research. Further analysis revealed that impairment of pyrimidine and glutathione metabolisms and immune function may be critical for hepatic aging and may provide targets for aging interventions.
Metabolome and Transcriptome Analyses of Red-colored Leaves From Eucommia Ulmoides ‘Huazhong No. 12’
Authors: Yang Yun , Chen Jiao Meng , Ding Huan Huan , Zhu Jing Le , Du Yan Hong
Published: November 2020

Background: Eucommia ulmoides ‘Oliver’ is an economically important tree species with highly medicinal and ecological values that is naturally distributed in China. E. ulmoides ‘Huazhong No. 12’ (H12) is the only red-leaf genotype of this species. In this study, the pigment contents of H12 and E. ulmoides ‘Huazhong No. 11’ (H11, green leaves) were determined. The differential metabolites in H12 and H11 were detected by UPLC-MS/MS, and the differentially expressed genes were screened by transcriptome. Then the key metabolites and corresponding gene regulation in anthocyanin related metabolic pathway were analyzed.Results: The chlorophyll a, chlorophyll b and carotenoid contents in H12 leaves were lower than H11, while the total anthocyanin content in H12 was 4.06 times higher than in H11. There were 96 up-regulated metabolites in H12, including anthocyanin, proanthocyanidins, flavonoid and flavonol. Among them, four differentially expressed anthocyanins were identified. A total of 8,368 differentially expressed genes were selected from transcriptome between H12 and H11. The flavone and flavonol biosynthesis pathway, anthocyanin pathway and photosynthetic pathway were analyzed. Finally, EuCHI, EuF3'H, EuF3'5'H, EuDFR and Eu3MaT1 were recommended as the key genes. The cyanidin, cyanidin 3-malonyl-glucoside and cyanidin 3, 5-glucoside were responsible for the H12 red leaves.Conclusion: This study revealed the metabolites and gene regulation of anthocyanin synthesis, and the potential function between the anthocyanin and photosynthetic gene expression in E. ulmoides red leaves. Notably, the results also provided a reference for the study of other plant leaf-coloring mechanisms.
Transcriptomic and Genome-wide Association Study Reveal Long Noncoding RNAs Responding to Nitrogen Deficiency in Maize
Authors: MA PENG , Zhang Xiao , Luo Bowen , Chen Zhen , He Xuan , Zhang Haiying , Li Binyang , Liu Dan , Wu Ling , Gao Shiqiang , Gao Duojiang , Zhang Suzhi , Gao Shibin
Published: November 2020

Background: Long noncoding RNAs (lncRNAs) play important roles in essential biological processes. However, our understanding of lncRNAs as competing endogenous RNAs (ceRNAs) and their responses to nitrogen stress is still limited.Results: Here, we surveyed the lncRNAs and miRNAs in maize inbred line P178 leaves and roots at the seedling stage under high-nitrogen and low-nitrogen conditions using lncRNA-Seq and small RNA-Seq. A total of 894 differentially expressed lncRNAs and 38 different miRNAs were identified. Co-expression analysis found two lncRNAs and four lncRNA-targets could competitively combine with ZmmiR159 and ZmmiR164, respectively. To dissect the genetic regulatory by which lncRNAs might enable adaptation to limited nitrogen availability. An association mapping panel containing a high-density single–nucleotide polymorphism (SNP) array (56,110 SNPs) combined with variable LN resistance-related phenotypes obtained from hydroponics was used for a genome-wide association study (GWAS). By combining GWAS and RNA-Seq, 170 differently expressed lncRNAs within the range of significant markers were screened. Moreover, 40 consistently LN-responsive genes including those involved in glutamine biosynthesis and nitrogen acquisition in root were identified. Transient expression assays in Nicotiana benthamiana demonstrated LNC_002923 could inhabit ZmmiR159-guided cleavage of Zm00001d015521. Conclusions: These lncRNAs containing trait-associated significant SNPs could consider to be related to root development and nutrient utilization. Taken together, the results of our study can provide new insights into the potential regulatory roles of lncRNAs in response to LN stress, and give valuable information for further screening of candidates as well as the improvement of maize regarding LN-responsive resistance.
Prevalence and Prognostic Significance of Hyponatremia in Lung Cancer Patients: Systematic Review and Meta-Analysis
Authors: Bartalis Eszter , Gergics Marin , Tinusz Benedek , Földi Mária , Kiss Szabolcs , Németh Dávid , Solymár Margit , Szakács Zsolt , Hegyi Péter , Mezősi Emese , Bajnok Laszlo
Published: November 2020

Background The prevalence of hyponatremia is highly variable among lung cancer patients. It is also an important predictive factor, according to numerous studies. However, its prevalence and prognostic significance in subgroups of lung cancer patients, e.g. in small cell and non-small cell lung cancers (SCLC and NSCLC, respectively), have not yet been evaluated in a meta-analysis.Methods We report this systematic review and meta-analysis according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA 2009) Statement. We have registered our meta-analysis and review’s protocol to the PROSPERO International Prospective Register of Systematic Reviews, with the following registration number: CRD42020167013. A systematic search was done in the following sources: MEDLINE, Embase, CENTRAL, Web of Science, ClinicalTrials.gov, a WHO Global Health Library. We extracted the effect measure for each outcome as the relative risk (RR) with the related 95% confidence interval (CI). Results We identified a total of 8127 potentially eligible studies and we included 25 studies in our evaluation. The prevalence of hyponatremia in lung cancer patients varied between 3% and 56.1% with an average of 23% without any significant differences between the following subgroups: cancer type (p=0.780), gender (p=0.223), age (p=0.773), ECOG state (p=0.317) and the extent of disease (p=0.999). Hyponatremia was more consistently an independent prognostic factor in NSCLC than in SCLC. The overall survival (OS) was significantly lower in hyponatremic compared to normonatremic patients at 10 months (RR: 0.59, 95% CI, 0.47 to 0.74, p<0.001) and at 20 months (RR: 0.44, 95% CI, 0.33 to 0.59, p<0.001), with worse survival rates in NSCLC than in SCLC (27% vs 42%) (p<0.001). If hyponatremia was corrected, OS at 10 months was 1.83 times higher than in the uncorrected hyponatremia group (95% CI, 1.37 to 2.44, p<0.001), but at 20 months no statistically significant difference could be found between these subgroups (p=0.067).Conclusions Low serum sodium levels have a negative impact on mortality at 10 and 20 months, which is more pronounced among NSCLC patients. The correction of hyponatremia has a positive effect on OS rates at 10 months, but this advantage disappears by 20 months.
Current Lung Cancer Screening Guidelines may Miss the High-risk Population in the Real-world Physical Examination Population: A Large Prospective Study
Authors: Ji Guiyi , Bao Ting , Li Zhenzhen , Tang Huairong , Liu Dan , Yang Ping , Li Weimin , Huang Yan
Published: November 2020

Background: Despite much research published on lung cancer screening, China has had no large-scale study on the missed diagnosis of lung cancer in physical examination population. We therefore did a real-world study using the current lung cancer screening guidelines to the physical examination population in China to determine the proportion of lung cancer cases that have been missed.Methods: A real-world prospective cohort study of screening, with the use of low-dose computed tomography, was conducted among people who took yearly health checkup in health management center of West China Hospital between 2006 and 2017. We respectively used current guidelines including lung cancer screening guidelines of the U.S. Preventive Services Task Force (USPSTF) and expert consensus on low dose spiral CT lung cancer screening in China. Results: In a total of 15996 participants with physical examination who completed the baseline screening, 6779 (42.4%) subjects had at least one positive finding, and 142 (2.1%) cases of lung cancer were screened positive. The false positive rate was 97.9%. Of 142 lung cancer cases detected in our study, only 9.2% met the lung cancer screening guidelines proposed by the USPSTF, and 24.4% met that of China. The rates of missed diagnosis were as high as 90.8% and 75.6% respectively. In addition, we did an in-depth analysis by gender. We found that among male lung cancer patients, the proportion of smokers was 75%, and the proportion of young people under 50 was 23.2%. Among female patients with lung cancer, the proportion of smokers was only 5.8%, and the proportion of young people under 50 was up to 33.3%.Conclusions: The rate of missed diagnosis was as high as 90.8% applying the current lung cancer screening guidelines to the physical examination population in China. Further study to determine screening guidelines for targeted populations, is warranted.
Screening is Associated with Lower Mastectomy Rates in Eastern Switzerland Beyond Stage Effects
Authors: Herrmann Christian , Morant Rudolf , Walser Esther , Mousavi Mohsen , Thürlimann Beat
Published: November 2020

ObjectivesA recent study found an influence of organized mammography screening programmes (MSPs) on geographical and temporal variation of mastectomy rates. We aimed to quantify the effect on the example of one of the cantonal programmes in Switzerland.MethodsWe used incidence data for the years 2010-2017 from the cancer registry of Eastern Switzerland. We included women with invasive-non-metastatic breast cancer (BC) in the screening age group 50-69-year-olds in the canton of St.Gallen. We compared mastectomy rates among cancer patients detected through the organised screening programme (MSP) vs. otherwise detected by stage.ResultsMSP-detected patients in St.Gallen presented with lower stages. 95% of MSP-detected had stages I-II vs 76% of Non-MSP-detected. Within all non-metastatic stage, tumour size and nodal status groups, MSP-detected patients had lower mastectomy rates, overall 10% vs 24% in 50-69-year-old non-participants. Their odds of receiving a mastectomy are about half of the Non-MSP-detected (OR=0.48, p=0.002).ConclusionsOur study showed that MSPs have a positive effect on lowering mastectomy rates. Screening participants are significantly less likely to receive a mastectomy compared to non-participants, which must be attributed to additional factors than just lower stages. Lower mastectomy rates lead to a higher quality of life for many patients.
Coagulopathy and Its Associated Factors Among Bleeding Diathesis Patients Attending at University of Gondar Specialized Referral Hospital, Northwest Ethiopia.
Authors: Aynalem Melak , Shiferaw Elias , Gelaw Yemataw , Enawgaw Bamlaku
Published: November 2020

Background: Coagulopathy is the major cause of mortality and morbidity throughout the world. Globally, about 26 - 45% of healthy people have a history of bleeding symptoms, which may be a result of thrombocytopenia, factor deficiency, or pathological inhibitory.Objective: To assess coagulopathy and its associated factors among bleeding diathesis patients attending at University of Gondar Specialized Referral Hospital from January to May 2020.Method: A cross-sectional study was conducted on 384 study participants with bleeding diathesis recruited by using a convenient sampling technique. Socio-demographic and clinical characteristics were collected by using questioners. Then 6ml venous blood was collected with a needle and syringe method. About 3ml blood was transferred to EDTA test tube for platelet count and 2.7ml blood was transferred to a test tube containing 0.3ml of 3.2% sodium citrated anticoagulant for coagulation test. For those study participants with prolonged coagulation tests, a mixing test was done to differentiate the causes of coagulopathy whether factor deficiency or inhibitors. Blood film and stool examination were also done for malaria and intestinal parasite identification, respectively. The data were entered into EPI-Info version 3.5.3 and then transferred to SPSS version-20 for analysis. Descriptive statistics were summarized as percentages, means, and standard deviations. Bivariate and multivariate logistic regression was used to identify the associated factors, and a P-value less than 0.05 was considered as statistically significant.Results: In this study, the prevalence of coagulopathy was 253/384 (65.9%; 95% CI: 61.16, 70.64). From them, 21.3% (54/253), 51.4% (130/253), and 27.3% (69/253) had only thrombocytopenia, only prolonged coagulation test, and mixed abnormality, respectively. Among participants with prolonged coagulation time, the prevalence of factor deficiency was 21.1% (42/199). Cardiac disease (AOR = 4.80; 95% CI: 2.65, 23.1), and other chronic diseases (AOR = 8.1; 95% CI: 1.84, 35.58) were significantly associated with coagulopathy.Conclusion: Coagulopathy due to inhibitory was a public health problem. The participant with cardiac and other chronic disease was associated with coagulopathy. Therefore, mixing tests could done for all prolonged coagulation test and it could be considered as a routine laboratory test.