bluuebunny/crossref_metadata_2025 huggingface dataset:
The genetic and environmental etiology of child maltreatment in a parent-based extended family design
Authors: Pittner Katharina , van Ijzendoorn Marinus H. , Alink Lenneke R. A. , Buisman Renate S. M. , Compier-de Block Laura H. C. G. C. , van den Berg Lisa J. M. , Elzinga Bernet M. , Lindenberg Jolanda , Tollenaar Marieke S. , Diego Vincent P. , Bakermans-Kranenburg Marian J.
Published: February 2019

Child maltreatment has been associated with various cumulative risk factors. However, little is known about the extent to which genetic and environmental factors contribute to individual differences between parents in perpetrating child maltreatment. To estimate the relative contribution of genetic and environmental factors to perpetrating maltreatment we used a parent-based extended family design. Child-reported perpetrated maltreatment was available for 556 parents (283 women) from 63 families. To explore reporter effects (i.e., child perspective on maltreatment), child reports were compared to multi-informant reports. Based on polygenic model analyses, most of the variance related to the perpetration of physical abuse and emotional neglect was explained by common environmental factors (physical abuse: c2 = 59%, SE = 12%, p = .006; emotional neglect: c2 = 47%, SE = 8%, p < .001) whereas genetic factors did not significantly contribute to the model. For perpetrated emotional abuse, in contrast, genetic factors did significantly contribute to perpetrated emotional abuse (h2 = 33%, SE = 8%, p < .001), whereas common environment factors did not. Multi-informant reports led to similar estimates of genetic and common environmental effects on all measures except for emotional abuse, where a multi-informant approach yielded higher estimates of the common environmental effects. Overall, estimates of unique environment, including measurement error, were lower using multi-informant reports. In conclusion, our findings suggest that genetic pathways play a significant role in perpetrating emotional abuse, while physical abuse and emotional neglect are transmitted primarily through common environmental factors. These findings imply that interventions may need to target different mechanisms dependings on maltreatment type.
Electrophysiology finds no inherent delay for grammatical gender retrieval in non-native production
Authors: Shantz Kailen , Tanner Darren
Published: February 2019

Late second language (L2) learners experience pervasive difficulty mastering grammatical gender, and a comprehensive account of this deficit has yet to emerge. We investigate a previously unexamined aspect of L2 gender use: the time course of lexical feature retrieval. Using event-related potentials (ERPs) with a covert production task, we examined whether L2 gender retrieval is delayed relative to phonology and to the time course of feature retrieval in native speakers for familiar nouns whose gender participants had strong knowledge of. Results find that L2 gender retrieval is not fundamentally delayed, and that L2 lexical feature retrieval may be more susceptible to top-down influences. These findings place important constraints on accounts of L2 acquisition and processing with respect to how lexical features are represented and retrieved. Our results further suggest that deficits in online L2 gender use may stem from post-retrieval processes and/or retrieval errors rather than inherent delays in gender retrieval.
Coverage and factors associated with vitamin A supplementation among children aged 6–59 months in twenty-three sub-Saharan African countries
Authors: Berde Anselm S , Bester Petra , Kruger Iolanthé M
Published: February 2019

Objective

The present study aimed to give an overall view of the pattern of high-dose vitamin A supplementation (VAS) coverage in twenty-three sub-Saharan African countries and factors associated with receipt of VAS among children aged 6–59 months.

Design

Cross-sectional data from the twenty-three Demographic and Health Surveys conducted from 2011 to 2015 in twenty-three sub-Saharan African countries were pooled. A multilevel logistic regression model was used to explore factors associated with VAS.

Setting

Twenty-three sub-Saharan African countries.

Participants

Children (n215 511) aged 6–59 months.

Results

The overall coverage of VAS among children aged 6–59 months for the surveys included was 59·4 %. In the multivariable analysis, children whose mothers had primary (adjusted OR (aOR)=1·43; 95 % CI 1·39, 1·47) or secondary or above (aOR=1·72; 95 % CI 1·67, 1·77) educational status were more likely to receive VAS than children whose mothers had no formal education. Other factors associated with significantly increased likelihood of VAS were: living in urban areas; children of working mothers; children whose mothers had higher media exposure; children of older mothersv. children of mothers aged 15–19 years; and older childrenv. children aged 6–11 months. At the country level, lower media exposure was significant and negatively associated with VAS.

Conclusions

Broader VAS coverage is needed according to our data. More efforts are needed to scale up coverage, focusing mostly on groups at risk of non-receipt of vitamin A.
Non-Party Government: Bipartisan Lawmaking and Party Power in Congress
Authors: Curry James M. , Lee Frances E.
Published: February 2019

Majority leaders of the contemporary Congress preside over parties that are more cohesive than at any point in the modern era, and power has been centralized in party leadership offices. Do today’s majority parties succeed in enacting their legislative agendas to a greater extent than the less-cohesive parties of earlier eras? To address this question, we examine votes on all laws enacted from 1973–2016, as well as on the subset of landmark laws identified by Mayhew. In addition, we analyze the efforts of congressional majority parties to pass their agendas from 1985 to 2016. We find that enacting coalitions in recent congresses are nearly as bipartisan as they were in the 1970s. Most laws, including landmark enactments, continue to garner substantial bipartisan support. Furthermore, majority parties have not gotten better at passing their legislative programs. Contemporary congressional majorities actually fail on their agenda items at somewhat higher rates than the less-cohesive majority parties of the 1980s and 1990s. When majority parties succeed on their agenda priorities, they usually do so with support from a majority of the minority party in at least one chamber and with the endorsement of one or more of the minority party’s top leaders.
Are Saturn's Interchange Injections Organized by Rotational Longitude?
Authors: Azari A. R. , Jia X. , Liemohn M. W. , Hospodarsky G. B. , Provan G. , Ye S.‐Y. , Cowley S. W. H. , Paranicas C. , Sergis N. , Rymer A. M. , Thomsen M. F. , Mitchell D. G.
Published: February 2019

Saturn's magnetosphere has been extensively studied over the past 13 years with the now retired Cassini mission. Periodic modulations in a variety of magnetospheric phenomena have been observed at periods close to those associated with the emission intensity of Saturn kilometric radiation (SKR). Resulting from Rayleigh‐Taylor like plasma instabilities, interchange is believed to be the main plasma transport process in Saturn's inner to middle magnetosphere. Here we examine the organization of equatorially observed interchange events identified based on high‐energy (3–22 keV) H+ intensifications by several longitude systems that have been derived from different types of measurements. The main question of interest here is as follows: Do interchange injections undergo periodicities similar to the Saturn kilometric radiation or other magnetospheric phenomena? We find that interchange shows enhanced occurrence rates in the northern longitude systems between 30° and 120°, particularly between 7 and 9 Saturn Radii. However, this modulation is small compared to the organization by local time. Additionally, this organization is weak and inconsistent with previous findings based on data with a limited time span.
Soil Respiration Components and their Temperature Sensitivity Under Chemical Fertilizer and Compost Application: The Role of Nitrogen Supply and Compost Substrate Quality
Authors: Chen Zengming , Xu Yehong , Castellano Michael J. , Fontaine Sébastien , Wang Weijin , Ding Weixin
Published: February 2019

Understanding autotrophic (Ra) and heterotrophic (Rh) components of soil respiration (Rs) and their temperature sensitivity (Q10) is critical for predictingsoil carbon (C) cycle and its feedback to climate change. In agricultural systems, these processes can be considerably altered by chemical fertilizer and compost application due to changes in nitrogen (N) supply and substrate quality (decomposability). We conducted a field experiment including control, urea, and four compost treatments. Ra and Rh were separated using the root exclusion method. Composts were characterized by chemical analyses, 13C solid‐state nuclear magnetic resonance, and lignin monomers. Annual cumulative Ra, along with root biomass, increased with soil mineral N, while Rh was suppressed by excessive N supply. Thus, Ra was stimulated but Rh was decreased by urea alone application. Annual Rh was increased by application of compost, especially that containing most lignin vanillyl and syringyl units, O‐alkyl C, di‐O‐alkyl C, and manganese. However, during the initial period, Rh was most effectively stimulated by the compost containing most carbohydrates, lignin cinnamyl units, phenolic C, and calcium. Ra was mediated by N release from compost decomposition and thus exhibited similar responses to compost quality as Rh. The Rh Q10 was reduced, while Ra Q10was increased by chemical fertilizer and compost application. Moreover, the Rh Q10 negatively related to soil mineral N supply and compost indicators referring to high substrate quality. Overall, our results suggest that N supply and substrate quality played an important role in regulating soil C flux and its response to climate warming.
Loss of MADD expression inhibits cellular growth and metastasis in anaplastic thyroid cancer
Authors: Saini Shikha , Sripada Lakshmi , Tulla Kiara , Kumar Prabhakaran , Yue Fei , Kunda Nicholas , Maker Ajay V. , Prabhakar Bellur S.
Published: February 2019

Anaplastic Thyroid Cancer (ATC) is an aggressive malignancy with limited therapeutic options and dismal patient survival. We have previously shown MADD to be differentially overexpressed in multiple cancer histologies and to contribute to tumor cell growth and survival. Therefore, we targeted MADD by gene silencing, explored its effect on cellular proliferation and metastases and examined its therapeutic potential in an orthotopic ATC model in athymic nude mice. When compared to untreated control and scramble siRNA, MADD siRNA treatment inhibited the proliferative capacity of 8505C, C643 and HTH7 cells in vitro and 8505C-derived-orthotopic tumor growth in vivo. MADD ablation caused a significant reduction in cellular migration and invasion potential; clonogenic capacity; as well as, mitochondrial length and potential in vitro. This MADD siRNA-induced anti-migratory/invasive effect corresponded with inhibition of epithelial–mesenchymal transition (EMT) and Wnt signaling. Mechanistically, MADD siRNA inhibited TNFα induced activation of pERK, pGSK3β and β-catenin, suggesting that MADD knockdown might exert its anti-migratory/invasive effects, by blocking TNFα/ERK/GSK3β axis. MADD siRNA can inhibit β-catenin nuclear translocation and consequently, the expression of its target genes in ATC cells. In in vivo experiments, along with tumor regression, MADD siRNA treatment also decreased evidence of lung metastases. Immunohistochemically, MADD siRNA-treated tumor tissues exhibited a reduction in Ki67 and N-Cadherin expression, and an increase in E-Cadherin expression. In conclusion, we show the crucial role of MADD in ATC tumorigenesis and metastasis and its potential implications as a molecular target for ATC therapy.
Bcl9 and Pygo synergise downstream of Apc to effect intestinal neoplasia in FAP mouse models
Authors: Mieszczanek Juliusz , van Tienen Laurens M. , Ibrahim Ashraf E. K. , Winton Douglas J. , Bienz Mariann
Published: February 2019

Bcl9 and Pygo are Wnt enhanceosome components that effect β-catenin-dependent transcription. Whether they mediate β-catenin-dependent neoplasia is unclear. Here we assess their roles in intestinal tumourigenesis initiated by Apc loss-of-function (ApcMin), or by Apc1322T encoding a partially-functional Apc truncation commonly found in colorectal carcinomas. Intestinal deletion of Bcl9 extends disease-free survival in both models, and essentially cures Apc1322T mice of their neoplasia. Loss-of-Bcl9 synergises with loss-of-Pygo to shift gene expression within Apc-mutant adenomas from stem cell-like to differentiation along Notch-regulated secretory lineages. Bcl9 loss also promotes tumour retention in ApcMin mice, apparently via relocating nuclear β-catenin to the cell surface, but this undesirable effect is not seen in Apc1322T mice whose Apc truncation retains partial function in regulating β-catenin. Our results demonstrate a key role of the Wnt enhanceosome in β-catenin-dependent intestinal tumourigenesis and reveal the potential of BCL9 as a therapeutic target during early stages of colorectal cancer.
Longitudinal HIV sequencing reveals reservoir expression leading to decay which is obscured by clonal expansion
Authors: Pinzone Marilia Rita , VanBelzen D. Jake , Weissman Sam , Bertuccio Maria Paola , Cannon LaMont , Venanzi-Rullo Emmanuele , Migueles Stephen , Jones R. Brad , Mota Talia , Joseph Sarah B. , Groen Kevin , Pasternak Alexander O. , Hwang Wei-Ting , Sherman Brad , Vourekas Anastasios , Nunnari Giuseppe , O’Doherty Una
Published: February 2019

After initiating antiretroviral therapy (ART), a rapid decline in HIV viral load is followed by a long period of undetectable viremia. Viral outgrowth assay suggests the reservoir continues to decline slowly. Here, we use full-length sequencing to longitudinally study the proviral landscape of four subjects on ART to investigate the selective pressures influencing the dynamics of the treatment-resistant HIV reservoir. We find intact and defective proviruses that contain genetic elements favoring efficient protein expression decrease over time. Moreover, proviruses that lack these genetic elements, yet contain strong donor splice sequences, increase relatively to other defective proviruses, especially among clones. Our work suggests that HIV expression occurs to a significant extent during ART and results in HIV clearance, but this is obscured by the expansion of proviral clones. Paradoxically, clonal expansion may also be enhanced by HIV expression that leads to splicing between HIV donor splice sites and downstream human exons.
Efficient metal halide perovskite light-emitting diodes with significantly improved light extraction on nanophotonic substrates
Authors: Zhang Qianpeng , Tavakoli Mohammad Mahdi , Gu Leilei , Zhang Daquan , Tang Lei , Gao Yuan , Guo Ji , Lin Yuanjing , Leung Siu-Fung , Poddar Swapnadeep , Fu Yu , Fan Zhiyong
Published: February 2019

Metal halide perovskite has emerged as a promising material for light-emitting diodes. In the past, the performance of devices has been improved mainly by optimizing the active and charge injection layers. However, the large refractive index difference among different materials limits the overall light extraction. Herein, we fabricate efficient methylammonium lead bromide light-emitting diodes on nanophotonic substrates with an optimal device external quantum efficiency of 17.5% which is around twice of the record for the planar device based on this material system. Furthermore, optical modelling shows that a high light extraction efficiency of 73.6% can be achieved as a result of a two-step light extraction process involving nanodome light couplers and nanowire optical antennas on the nanophotonic substrate. These results suggest that utilization of nanophotonic structures can be an effective approach to achieve high performance perovskite light-emitting diodes.
A meiosis-specific BRCA2 binding protein recruits recombinases to DNA double-strand breaks to ensure homologous recombination
Authors: Zhang Jingjing , Fujiwara Yasuhiro , Yamamoto Shohei , Shibuya Hiroki
Published: February 2019

Homologous recombination (HR) repairs DNA double-strand breaks (DSBs) to maintain genomic integrity. Recombinase recruited to the DSBs by the mediator protein BRCA2 catalyzes the homology-directed repair. During meiotic HR, programmed DSBs are introduced genome-wide but their repair mechanisms, including the regulation of BRCA2, have remained largely elusive. Here we identify a meiotic localizer of BRCA2, MEILB2/HSF2BP, that localizes to the site of meiotic DSBs in mice. Disruption ofMeilb2abolishes the localization of RAD51 and DMC1 recombinases in spermatocytes, leading to errors in DSB repair and male sterility. MEILB2 directly binds to BRCA2 and regulates its association to meiotic DSBs. We map the MEILB2-binding domain within BRCA2 that is distinct from the canonical DNA-binding domain but is sufficient to localize to meiotic DSBs in a MEILB2-dependent manner. We conclude that localization of BRCA2 to meiotic DSBs is mediated by MEILB2, which is an integral mechanism to repair abundant meiotic DSBs.
Seagrass can mitigate negative ocean acidification effects on calcifying algae
Authors: Bergstrom Ellie , Silva João , Martins Cíntia , Horta Paulo
Published: February 2019

The ultimate effect that ocean acidification (OA) and warming will have on the physiology of calcifying algae is still largely uncertain. Responses depend on the complex interactions between seawater chemistry, global/local stressors and species-specific physiologies. There is a significant gap regarding the effect that metabolic interactions between coexisting species may have on local seawater chemistry and the concurrent effect of OA. Here, we manipulated CO2 and temperature to evaluate the physiological responses of two common photoautotrophs from shallow tropical marine coastal ecosystems in Brazil: the calcifying alga Halimeda cuneata, and the seagrass Halodule wrightii. We tested whether or not seagrass presence can influence the calcification rate of a widespread and abundant species of Halimeda under OA and warming. Our results demonstrate that under elevated CO2, the high photosynthetic rates of H. wrightii contribute to raise H. cuneata calcification more than two-fold and thus we suggest that H. cuneata populations coexisting with H. wrightii may have a higher resilience to OA conditions. This conclusion supports the more general hypothesis that, in coastal and shallow reef environments, the metabolic interactions between calcifying and non-calcifying organisms are instrumental in providing refuge against OA effects and increasing the resilience of the more OA-susceptible species.
Variations in hydrodynamic characteristics of netting panels with various twine materials, knot types, and weave patterns at small attack angles
Authors: Tang Hao , Hu Fuxiang , Xu Liuxiong , Dong Shuchuang , Zhou Cheng , Wang Xuefang
Published: February 2019

It is essential to conduct hydrodynamic experiments for fishing gear at small attack angles along the flow direction to better understand the hydrodynamic characteristics of netting and application of gear. The hydrodynamic characteristics of netting panels made of different materials at small attack angles were investigated by a self-designed setup; this is essential for the effective use of netting on different types of gears. As confirmed by experiments, the measured drag of designed frame without netting accounted for less than 20% of the total setup drag including experimental netting and remained in a steady state under various current speeds and small attack angles, indicating that the self-designed frame setup is suitable for such trials. The drag coefficient was determined by varying the attack angle, solidity ratio, Reynolds number, knot types, weave pattern, and twine materials at small attack angles. The results indicate that the drag coefficient increased as the attack angle increased, but decreased as the solidity ratio and Reynolds number increased. The drag generated by knot accounted for 21% of the total drag of nylon (PA) netting. For braided knotless netting, the drag coefficient of PA netting was about 8.4% lower than that of polythene netting (PE) and 7% lower than that of polyester netting (PES). Compared with twined netting, the braided netting exhibited a higher resistance to flow, corresponding to higher values of drag coefficient.
Precise Slow Oscillation–Spindle Coupling Promotes Memory Consolidation in Younger and Older Adults
Authors: Muehlroth Beate E. , Sander Myriam C. , Fandakova Yana , Grandy Thomas H. , Rasch Björn , Shing Yee Lee , Werkle-Bergner Markus
Published: February 2019

Memory consolidation during sleep relies on the precisely timed interaction of rhythmic neural events. Here, we investigate differences in slow oscillations (SO; 0.5–1 Hz), sleep spindles (SP), and their coupling across the adult human lifespan and ask whether observed alterations relate to the ability to retain associative memories across sleep. We demonstrate that older adults do not show the fine-tuned coupling of fast SPs (12.5–16 Hz) to the SO peak present in younger adults but, instead, are characterized most by a slow SP power increase (9–12.5 Hz) at the end of the SO up-state. This slow SP power increase, typical for older adults, coincides with worse memory consolidation in young age already, whereas the tight precision of SO–fast SP coupling promotes memory consolidation across younger and older adults. Crucially, brain integrity in source regions of SO and SP generation, including the medial prefrontal cortex, thalamus, hippocampus and entorhinal cortex, reinforces this beneficial SO–SP coupling in old age. Our results reveal that cognitive functioning is not only determined by maintaining structural brain integrity across the adult lifespan, but also by the preservation of precisely timed neural interactions during sleep that enable the consolidation of declarative memories.
Prebiotic chemistry in neutral/reduced-alkaline gas-liquid interfaces
Authors: Mompeán Cristina , Marín-Yaseli Margarita R. , Espigares Patricia , González-Toril Elena , Zorzano María-Paz , Ruiz-Bermejo Marta
Published: February 2019

The conditions for the potential abiotic formation of organic compounds from inorganic precursors have great implications for our understanding of the origin of life on Earth and for its possible detection in other environments of the Solar System. It is known that aerosol-interfaces are effective at enhancing prebiotic chemical reactions, but the roles of salinity and pH have been poorly investigated to date. Here, we experimentally demonstrate the uniqueness of alkaline aerosols as prebiotic reactors that produce an undifferentiated accumulation of a variety of multi-carbon biomolecules resulting from high-energy processes (in our case, electrical discharges). Using simulation experiments, we demonstrate that the detection of important biomolecules in tholins increases when plausible and particular local planetary environmental conditions are simulated. A greater diversity in amino acids, carboxylic acids, N-heterocycles, and ketoacids, such as glyoxylic and pyruvic acid, was identified in tholins synthetized from reduced and neutral atmospheres in the presence of alkaline aqueous aerosols than that from the same atmospheres but using neutral or acidic aqueous aerosols.
Induction of MET Receptor Tyrosine Kinase Down-regulation through Antibody-mediated Receptor Clustering
Authors: Li Wenjing , Dick Adam , Lu Fei , Zhang Hui , Sun Hong
Published: February 2019

The proto-oncoprotein MET is a receptor tyrosine kinase that plays a key role in cancer cell growth and invasion. We have used fluorescence-tagged antibodies to activate MET in live serum-starved glioblastoma cells and monitor the fate of antibody-bound MET receptor in single cell-based assays. We found that the antibodies induced rapid and transient formation of highly polarized MET clusters on the plasma membrane and promoted the activation of MET, resembling the initial effects of binding to its ligand, HGF. However, the antibody-induced clustering and activation of MET led to the rapid removal of the receptor from cell surface and altered its intracellular processing, resulted in rapid degradation of the receptor. Consequently, while cells pre-treated with HGF remain competent to respond to further HGF stimulation, cells pre-treated with antibodies are refractory to further HGF stimulation due to antibody-mediated MET depletion. Removal of MET by sustained treatment of antibodies blocked cancer cell migration and invasion. Our studies reveal a novel mechanism to alter the recycling process of MET in glioblastoma cancer cells by promoting the receptor degradation through a proteasome-sensitive and lysosome-dependent pathway through the ligand-independent activation of MET using anti-MET antibodies.
Anomalous orbital structure in two-dimensional titanium dichalcogenides
Authors: Pal Banabir , Cao Yanwei , Liu Xiaoran , Wen Fangdi , Kareev M. , N’Diaye A. T. , Shafer P. , Arenholz E. , Chakhalian J.
Published: February 2019

Generally, lattice distortions play a key role in determining the electronic ground states of materials. Although it is well known that trigonal distortions are generic to most two dimensional transition metal dichalcogenides, the impact of this structural distortion on the electronic structure and topological properties has not been understood conclusively. Here, by using a combination of polarization dependent X-ray absorption spectroscopy (XAS), X-ray photoelectron spectroscopy (XPS) and atomic multiplet cluster calculations, we have investigated the electronic structure of titanium dichalcogenides TiX2 (X = S, Se, Te), where the magnitude of the trigonal distortion increase monotonically from S to Se and Te. Our results reveal the presence of an anomalously large crystal field splitting. This unusual kind of crystal field splitting is likely responsible for the unconventional electronic structure of TiX2 compounds and ultimately controls the degree of the electronic phase protection. Our findings also indicate the drawback of the distorted crystal field picture in explaining the observed electronic ground state and emphasize the key importance of trigonal symmetry, metal-ligand hybridization and electron-electron correlations in defining the electronic structures at the Fermi energy.
Age-dependent differences in learning to control a robot arm using a body-machine interface
Authors: Ranganathan Rajiv , Lee Mei-Hua , Padmanabhan Malavika R. , Aspelund Sanders , Kagerer Florian A. , Mukherjee Ranjan
Published: February 2019

Body-machine interfaces, i.e. interfaces that rely on body movements to control external assistive devices, have been proposed as a safe and robust means of achieving movement and mobility; however, how children learn these novel interfaces is poorly understood. Here we characterized the learning of a body-machine interface in young unimpaired adults, two groups of typically developing children (9-year and 12-year olds), and one child with congenital limb deficiency. Participants had to control the end-effector of a robot arm in 2D using movements of the shoulder and torso. Results showed a striking effect of age - children had much greater difficulty in learning the task compared to adults, with a majority of the 9-year old group unable to even complete the task. The 12-year olds also showed poorer task performance compared to adults (as measured by longer movement times and greater path lengths), which were associated with less effective search strategies. The child with congenital limb deficiency showed superior task performance compared to age-matched children, but had qualitatively distinct coordination strategies from the adults. Taken together, these results imply that children have difficulty learning non-intuitive interfaces and that the design of body-machine interfaces should account for these differences in pediatric populations.
Lab-on-Fiber Nanoprobe with Dual High-Q Rayleigh Anomaly-Surface Plasmon Polariton Resonances for Multiparameter Sensing
Authors: Kim Hyun-Tae , Yu Miao
Published: February 2019

Surface plasmon resonance (SPR) based sensing is an attractive approach for realizing lab-on-fiber nanoprobes. However, simultaneous measurement of multiple parameters (e.g., refractive index and temperature) with SPR-based nanoprobes, although highly desirable, is challenging. We report a lab-on-fiber nanoprobe with dual high-Q Rayleigh anomaly (RA)-surface plasmon polariton (SPP) resonances for multiparameter sensing. To achieve high-Q RA-SPP resonance the nanoprobe employs a plasmonic crystal cavity enhanced by distributed Bragg reflector (DBR) gratings on the end-face of a single-mode optical fiber. By tailoring the grating periods of the plasmonic crystal cavity and DBRs, two spatially separated high-Q RA-SPP resonance modes are designed within a 50 nm spectral range in C + L band. The fabricated nanoprobe demonstrates two RA-SPP resonances near 1550 nm with high Q-factors up to 198. These two high-Q resonances are further showed to exhibit distinctive responses to the changes of refractive index and temperature, which enables simultaneous measurements of both parameters. The proposed lab-on-fiber nanoprobes will pave the way for realizing compact multiparameter sensing solutions compatible with optical communication infrastructures.
Kidney Stones and Risk of Osteoporotic Fracture in Chronic Kidney Disease
Authors: Han Seung Gyu , Oh Jieun , Jeon Hee Jung , Park Chan , Cho Jeonghwan , Shin Dong Ho
Published: February 2019

Osteoporotic fracture associated with calcium dysregulation is more common in patients with kidney stones. However, little is known about the association of kidney stones and bone health status in patients with chronic kidney disease (CKD). This retrospective medical record-based study included 2282 patients with stable stage 3–4 CKD between 2007 and 2017. Of these, 113 patients were diagnosed with kidney stones. Propensity score matching for 226 patients with and without kidney stones showed that osteoporotic fracture occurred more often in patients with kidney stones (33, 29.2%) than in patients without kidney stones (16, 14.2%), resulting in rates of 5.56 and 2.63/100 patient-years, respectively (p < 0.01). In particular, Cox proportional hazard analysis revealed that kidney stones were significantly associated with osteoporotic fracture, even after adjusting for age, sex, body mass index, kidney stones, estimated glomerular filtration rate, excessive alcohol consumption, current smoking, and steroid use in patients with CKD stage 3–4 (hazard ratio, 2.32, 95% CI 1.24–4.34, p = 0.01). This study showed that the presence of kidney stones was a significant predictor for osteoporotic fracture in patients with CKD, suggesting that it should be considered as a clinical risk factor for osteoporotic fracture in them.
Effects of running on adiponectin, insulin and cytokines in cerebrospinal fluid in healthy young individuals
Authors: Schön M. , Kovaničová Z. , Košutzká Z. , Nemec M. , Tomková M. , Jacková L. , Máderová D. , Slobodová L. , Valkovič P. , Ukropec J. , Ukropcová B.
Published: February 2019

Exercise can prevent the sedentary lifestyle-related risk of metabolic and cognitive decline, but mechanisms and mediators of exercise effects on human brain are relatively unexplored. We measured acute exercise-induced changes in adiponectin, insulin and other bioactive molecules in cerebrospinal fluid (CSF) and serum from young lean individuals. Samples of serum and CSF were obtained before and 1-h after the 90-min run (75–80% HRmax; maximal heart rate), additional serum was taken at finish-line. Body composition, physical fitness, metabolic rate, cognitive functions, food preference, glucose, insulin and albumin were measured. The spectrum of 174 cytokines was assessed by protein arrays, adiponectin was also determined by ELISA and immunoblotting. CSF adiponectin decreased post-exercise by 21.3% (arrays) and 25.8% (ELISA) (p < 0.009). Immunoblotting revealed reduction in a low-molecular-weight-adiponectin (p < 0.005). CSF adiponectin positively correlated with CSF/serum albumin ratio (p < 0.022), an indicator of blood-brain-barrier permeability. CSF and serum adiponectin were positively associated with memory and running-induced changes in insulinemia and CSF insulin. Additionally, running modulated CSF levels of 16 other cytokines. Acute running reduced CSF adiponectin and modulated insulin and albumin in CSF and serum. Associations of adiponectin with memory and metabolism indicate the potential role of this bioactive molecule in mediating exercise-induced adaptive response in human brain.
Comprehensive RNA sequencing and co-expression network analysis to complete the biosynthetic pathway of coumestrol, a phytoestrogen
Authors: Ha Jungmin , Kang Young-Gyu , Lee Taeyoung , Kim Myoyeon , Yoon Min Young , Lee Eunsoo , Yang Xuefei , Kim Donghyun , Kim Yong-Jin , Lee Tae Ryong , Kim Moon Young , Lee Suk-Ha
Published: February 2019

Coumestrol (CMS), a coumestan isoflavone, plays key roles in nodulation through communication with rhizobia, and has been used as phytoestrogens for hormone replacement therapy in humans. Because CMS content is controlled by multiple genetic factors, the genetic basis of CMS biosynthesis has remained unclear. We identified soybean genotypes with consistently high (Daewonkong) or low (SS0903-2B-21-1-2) CMS content over 2 years. We performed RNA sequencing of leaf samples from both genotypes at developmental stage R7, when CMS levels are highest. Within the phenylpropanoid biosynthetic pathway, 41 genes were tightly connected in a functional co-expression gene network; seven of these genes were differentially expressed between two genotypes. We identified 14 candidate genes involved in CMS biosynthesis. Among them, seven were annotated as encoding oxidoreductases that may catalyze the transfer of electrons from daidzein, a precursor of CMS. Two of the other genes, annotated as encoding a MYB domain protein and a MLP–like protein, may increase CMS accumulation in response to stress conditions. Our results will help to complete our understanding of the CMS biosynthetic pathway, and should facilitate development of soybean cultivars with high CMS content that could be used to promote the fitness of plants and human beings.
New 2-aryl-6-methyl-3,4-dihydro-β-carbolin-2-iums as potential antifungal agents: Synthesis, bioactivity and structure-activity relationship
Authors: Li Xingqiang , Zhang Bingyu , Zhao Wei , Yang Shanshan , Yang Xinjuan , Zhou Le
Published: February 2019

Thirty new title compounds along with five known analogues were prepared from commercially available 2-arylhydrazin-1-ium chlorides and α-ketoglutaric acid. The mycelium growth rate method was used to evaluate inhibition activity against six strains of plant pathogenic fungi. Most of the compounds displayed the activity for each the fungi at 150 μΜ, higher than azoxystrobin, a positive drug. Compound 6-2 showed the lowest average IC50 value of 4.58 μg/mL for all the fungi where F. solani exhibited the highest susceptibility to most of the compounds. For F. solani, some compounds were more active with IC50 values of 2.67–8.48 μM than thiabendazole (IC50 = 9.30 μM) and/or carbendazim (IC50 = 3.36 μM). The SAR showed that the activity is significantly affected by substituents on the A-ring and/or D-ring along with the degree of unsaturation of the C-ring. Thus, a series of new β-carboline compounds with potent antifungal potential were found.
Neuronal message passing using Mean-field, Bethe, and Marginal approximations
Authors: Parr Thomas , Markovic Dimitrije , Kiebel Stefan J. , Friston Karl J.
Published: February 2019

Neuronal computations rely upon local interactions across synapses. For a neuronal network to perform inference, it must integrate information from locally computed messages that are propagated among elements of that network. We review the form of two popular (Bayesian) message passing schemes and consider their plausibility as descriptions of inference in biological networks. These are variational message passing and belief propagation – each of which is derived from a free energy functional that relies upon different approximations (mean-field and Bethe respectively). We begin with an overview of these schemes and illustrate the form of the messages required to perform inference using Hidden Markov Models as generative models. Throughout, we use factor graphs to show the form of the generative models and of the messages they entail. We consider how these messages might manifest neuronally and simulate the inferences they perform. While variational message passing offers a simple and neuronally plausible architecture, it falls short of the inferential performance of belief propagation. In contrast, belief propagation allows exact computation of marginal posteriors at the expense of the architectural simplicity of variational message passing. As a compromise between these two extremes, we offer a third approach – marginal message passing – that features a simple architecture, while approximating the performance of belief propagation. Finally, we link formal considerations to accounts of neurological and psychiatric syndromes in terms of aberrant message passing.
Changes in rhizosphere bacterial communities during remediation of heavy metal-accumulating plants around the Xikuangshan mine in southern China
Authors: Guo Dongchu , Fan Zhouzhou , Lu Shuyu , Ma Yongjiao , Nie Xiaohong , Tong Fangping , Peng Xiawei
Published: February 2019

Mining and smelting activities are the major sources of antimony (Sb) contamination. The soil around Xikuangshan (XKS), one of the largest Sb mines in the world, has been contaminated with high concentrations of Sb and other associated metals, and has attracted extensive scholarly attention. Phytoremediation is considered a promising method for removing heavy metals, and the diversity and structure of rhizosphere microorganisms may change during the phytoremediation process. The rhizosphere microbiome is involved in soil energy transfer, nutrient cycling, and resistance and detoxification of metal elements. Thus, changes in this microbiome are worthy of investigation using high-throughput sequencing techniques. Our study in Changlongjie and Lianmeng around XKS revealed that microbial diversity indices in the rhizospheres of Broussonetia papyrifera and Ligustrum lucidum were significantly higher than in bulk soil, indicating that plants affect microbial communities. Additionally, most of the bacteria that were enriched in the rhizosphere belonged to the Proteobacteria, Acidobacteria, Actinobacteria, and Bacteroidetes. In Changlongjie and Lianmeng, the diversity and abundance of the microbial community in the B. papyrifera rhizosphere were higher than in L. lucidum. In parallel, the soil pH of the B. papyrifera rhizosphere increased significantly in acidic soil and decreased significantly in near-neutral soil. Redundancy analyses indicated that pH was likely the main factor affecting the overall bacterial community compositions, followed by moisture content, Sb, arsenic (As), and chromium (Cr).
Characterization of circulating miRNA signature in water buffaloes (Bubalus bubalis) during Brucella abortus infection and evaluation as potential biomarkers for non-invasive diagnosis in vaginal fluid
Authors: Lecchi Cristina , Catozzi Carlotta , Zamarian Valentina , Poggi Gaia , Borriello Giorgia , Martucciello Alessandra , Vecchio Domenico , DeCarlo Esterina , Galiero Giorgio , Ceciliani Fabrizio
Published: February 2019

Brucellosis is an infectious disease caused by bacteria from the Brucella genus that can be transmitted to humans through contact with infected animals or contaminated animal products. Brucellosis also causes financial losses in animal production. Ruminants are highly susceptible to brucellosis, and the causative agent water buffaloes (Bubalus bubalis) is Brucella abortus. Circulating microRNAs (miRNAs) are cropping up as promising biomarkers for several infectious diseases. The goals of this study were to characterize the serum miRNA signature associated with brucellosis in water buffaloes and investigate the miRNAs’ potential use as biomarkers in vaginal fluids. Next Generation Sequencing was used to assess miRNA expression profiles in Brucella-positive and Brucella-negative blood sera; dysregulated miRNAs in blood serum and vaginal fluids were validated using RT-qPCR. ROC curves were generated to evaluate the diagnostic value of miRNAs for Brucella. GO and KEGG pathway enrichment analyses were exploited to investigate the biological functions of dysregulated miRNAs. The results showed that 20 miRNAs were modulated, of which, 12 were upregulated and 8 were downregulated. These findings were corroborated by RT-qPCR, and ROC curves indicated that the miRNAs can serve as potential biomarkers for Brucella. GO and KEGG pathway analyses pointed out that some of these miRNAs are related to immune response and apoptosis. These results provided an overview of miRNA expression profiles and highlighted potential biomarkers for Brucella infection in water buffaloes. We also demonstrated the potential of vaginal fluids in studies involving microRNA detection. Further functional and mechanistic studies of these miRNAs may improve our understanding of the biological processes involved in Brucella infection and host immune response.
NMR Analysis on Molecular Interaction of Lignin with Amino Acid Residues of Carbohydrate-Binding Module from Trichoderma reesei Cel7A
Authors: Tokunaga Yuki , Nagata Takashi , Suetomi Takashi , Oshiro Satoshi , Kondo Keiko , Katahira Masato , Watanabe Takashi
Published: February 2019

Lignocellulosic biomass is anticipated to serve as a platform for green chemicals and fuels. Nonproductive binding of lignin to cellulolytic enzymes should be avoided for conversion of lignocellulose through enzymatic saccharification. Although carbohydrate-binding modules (CBMs) of cellulolytic enzymes strongly bind to lignin, the adsorption mechanism at molecular level is still unclear. Here, we report NMR-based analyses of binding sites on CBM1 of cellobiohydrolase I (Cel7A) from a hyper-cellulase-producing fungus, Trichoderma reesei, with cellohexaose and lignins from Japanese cedar (C-MWL) and Eucalyptus globulus (E-MWL). A method was established to obtain properly folded TrCBM1. Only TrCBM1 that was expressed in freshly transformed E. coli had intact conformation. Chemical shift perturbation analyses revealed that TrCBM1 adsorbed cellohexaose in highly specific manner via two subsites, flat plane surface and cleft, which were located on the opposite side of the protein surface. Importantly, MWLs were adsorbed at multiple binding sites, including the subsites, having higher affinity than cellohexaose. G6 and Q7 were involved in lignin binding on the flat plane surface of TrCBM1, while cellohexaose preferentially interacted with N29 and Q34. TrCBM1 used much larger surface area to bind with C-MWL than E-MWL, indicating the mechanisms of adsorption toward hardwood and softwood lignins are different.
Evaluation of an improved tool for non-invasive prediction of neonatal respiratory morbidity based on fully automated fetal lung ultrasound analysis
Authors: Burgos-Artizzu Xavier P. , Perez-Moreno Álvaro , Coronado-Gutierrez David , Gratacos Eduard , Palacio Montse
Published: February 2019

The objective of this study was to evaluate the performance of a new version of quantusFLM®, a software tool for prediction of neonatal respiratory morbidity (NRM) by ultrasound, which incorporates a fully automated fetal lung delineation based on Deep Learning techniques. A set of 790 fetal lung ultrasound images obtained at 24 + 0–38 + 6 weeks’ gestation was evaluated. Perinatal outcomes and the occurrence of NRM were recorded. quantusFLM® version 3.0 was applied to all images to automatically delineate the fetal lung and predict NRM risk. The test was compared with the same technology but using a manual delineation of the fetal lung, and with a scenario where only gestational age was available. The software predicted NRM with a sensitivity, specificity, and positive and negative predictive value of 71.0%, 94.7%, 67.9%, and 95.4%, respectively, with an accuracy of 91.5%. The accuracy for predicting NRM obtained with the same texture analysis but using a manual delineation of the lung was 90.3%, and using only gestational age was 75.6%. To sum up, automated and non-invasive software predicted NRM with a performance similar to that reported for tests based on amniotic fluid analysis and much greater than that of gestational age alone.
Reduced mitochondrial respiration and increased calcium deposits in the EDL muscle, but not in soleus, from 12-week-old dystrophic mdx mice
Authors: Gaglianone Rhayanna B. , Santos Anderson Teixeira , Bloise Flavia Fonseca , Ortiga-Carvalho Tania Maria , Costa Manoel Luis , Quirico-Santos Thereza , da Silva Wagner Seixas , Mermelstein Claudia
Published: February 2019

Mitochondria play an important role in providing ATP for muscle contraction. Muscle physiology is compromised in Duchenne muscular dystrophy (DMD) and several studies have shown the involvement of bioenergetics. In this work we investigated the mitochondrial physiology in fibers from fast-twitch muscle (EDL) and slow-twitch muscle (soleus) in the mdx mouse model for DMD and in control C57BL/10J mice. In our study, multiple mitochondrial respiratory parameters were investigated in permeabilized muscle fibers from 12-week-old animals, a critical age where muscle regeneration is observed in the mdx mouse. Using substrates of complex I and complex II from the electron transport chain, ADP and mitochondrial inhibitors, we found in the mdx EDL, but not in the mdx soleus, a reduction in coupled respiration suggesting that ATP synthesis is affected. In addition, the oxygen consumption after addition of complex II substrate is reduced in mdx EDL; the maximal consumption rate (measured in the presence of uncoupler) also seems to be reduced. Mitochondria are involved in calcium regulation and we observed, using alizarin stain, calcium deposits in mdx muscles but not in control muscles. Interestingly, more calcium deposits were found in mdx EDL than in mdx soleus. These data provide evidence that in 12-week-old mdx mice, calcium is accumulated and mitochondrial function is disturbed in the fast-twitch muscle EDL, but not in the slow-twitch muscle soleus.
Evidence of microalgal isotopic fractionation through enrichment of depleted uranium
Authors: Baselga-Cervera Beatriz , García-Balboa Camino , López-Rodas Victoria , Fernández Díaz Marta , Costas Eduardo
Published: February 2019

Resulting from the nuclear fuel cycle, large amounts of depleted uranium (DU) tails are piling up, waiting for possible use or final disposal. To date, the recovery of the residual 235U isotope contained in DU has been conducted only marginally by physical processes. Relative isotope abundances are often mediated by biological processes, and the biologically driven U isotopic fractionation has been previously identified in reducing bacteria. Our results indicate that the cells of two microalgal strains (freshwater Chlamydomonas sp. (ChlGS) and marine Tetraselmis mediterranea (TmmRU)) took up DU from the exposure solutions, inducing U isotopic fractionation with a preference for the fissile 235U isotope over 238U. The n(235U)/n(238U) isotopic fractionation magnitudes (δ235) were 23.6 ± 12.5‰ and 370.4 ± 103.9‰, respectively. These results open up new perspectives on the re-enrichment of DU tailings, offering a potential biological alternative to obtain reprocessed natural-equivalent uranium. Additionally, the findings present implications for identifying biological signatures in the geologic records.