bluuebunny/crossref_metadata_2025 huggingface dataset:
AVALIAÇÃO DO EQUILÍBRIO ESTÁTICO EM CRIANÇAS COM DEFICIÊNCIA VISUAL (DV)
Authors: Mosquera Carlos Fernando França , Souza Suellen da Costa , Souza William Cordeiro de , Nascimento Anne C.S.G. , Schlesener Anita Helena
Published: March 2023

A deficiência visual (DV) é definida como perda total ou parcial da visão, podendo ser congênita ou adquirida, provocada por doença como: toxoplasmose, retinopatia, glaucoma, catarata, oftalmia neonatal, traumatismos, entre outras ou ainda, por causas desconhecidas. É classificada em baixa visão ou visão subnormal e cegueira. De acordo com Smith (2008) e Oliveira et al (2000), pessoas com baixa visão podem ter eficiência no funcionamento visual, para isso, alguns fatores devem ser considerados, como o grau de acuidade visual, o uso da visão periférica (quando existente), e a etiologia da DV. A BV e a cegueira provocam na maioria das vezes um desequilí­brio nos deslocamentos dessas pessoas, como afirma a literatura, mas nem sempre estas informações são unânimes nas investigações cientí­ficas. Sabendo disso, traçamos como objetivo do trabalho a avaliação do perfil psicomotor (equilí­brio estático) de crianças entre 06 e 14 anos com BV (n=16)(GE) e sem a BV (n=26)(GC), usando dois testes de equilí­brio estático para reconhecer potencialidades e as dificuldades das crianças e comparando os resultados dos testes dos dois grupos. A opção pelos testes, decorre da facilidade de aquisição dos materiais, bem como o baixo custo para adquiri-los. Pode-se executar esses testes em qualquer espaço fí­sico, por isso a escolha de um método prático e viável. Em nossa pesquisa, a BV não representou de forma acurada a efetiva causa de desequilí­brios estáticos. Mas sim, podemos constatar que, crianças na faixa etária avaliada que exercitam-se extracurricular, obtém um melhor equilí­brio estático dos jovens que não se exercitam.
ANÁLISE DE CONTEÚDO DE PROCEDIMENTOS MUSICOTERAPÊUTICOSEM PACIENTES IDOSOS DA ATENÇÃO DOMICILIAR
Authors: Miranda Claudia Borges , Freire Marina Horta , Ribeiro Ana Paula , Vieira Samana Barbosa , Lopes Sheila Silveira Siao
Published: March 2023

O envelhecimento populacional e o aumento da prevalência de casos patológicos crônicos, com limitações fí­sicas, cognitivas e/ou sociais, associados às mudanças culturais da população, têm promovido diversas alterações nas formas de tratamento. A música tem sido cada vez mais reconhecida como um instrumento de grande potencial terapêutico, capaz de exercitar o paciente de forma única, já que seus elementos, como ritmo, harmonia e melodia, estimulam diversas partes do cérebro. Assim, a Musicoterapia surge como alternativa para a saúde integral na prevenção, na reabilitação, no tratamento de patologias e nos cuidados paliativos, principalmente para pacientes com idade avançada, sendo uma forma de tratamento ascendente na assistência domiciliar. Através de análise do conteúdo de relatórios e avaliações de sessões com pacientes domiciliares idosos foi possí­vel identificar oito dimensões da vida que podem ser beneficiadas com o trabalho de Musicoterapia. Foram também identificadas metas de tratamento personalizadas para cada paciente e propostos procedimentos musicoterapêuticos especí­ficos relacionados a cada dimensão da vida classificada. A aplicação dos procedimentos foi individualizada e observou-se expressivas melhoras nos pacientes atendidos.
"SOBRE A DOCÊNCIA EM MUSICOTERAPIA"
Authors: Barcellos Lia Rejane Mendes
Published: March 2023

O objetivo deste artigo é discorrer sobre a docência no Ensino Superior queé um dos três aspectos que fazem parte do tripé: ensino, pesquisa e extensão. Paraisto foi necessário investigar sobre o "Estado da Arte" da docência em musicoterapia esobre "˜como' os profissionais por ela responsáveis exercem essa função na formação domusicoterapeuta. Trata-se de tema ainda pouco discutido e que deveria ser consideradode extrema importância, na medida em que o docente é um dos principais agentes quecompartilham e disseminam o conhecimento, e um ator fundamental na formação domusicoterapeuta. Para a elaboração deste artigo foi realizado um levantamento sobre aquestão em pauta em fontes especí­ficas da área como: a Revista da UBAM (24 números,de 1996 a 2018), e a Revista Incantare (12 números, de 2010 a 2018), totalizando 306artigos, bem como em artigos e livros que fazem parte da literatura do campo, brasileira eestrangeira, tanto no que se refere à formação dos profissionais docentes, bem como sobreas estratégias por estes utilizadas, para que melhores resultados sejam alcançados.
LA RESISTENCIA AL NO VERBAL: DE LA MUSICOTERAPIA A LA TERAPIA BENENZON
Authors: Benenzon Rolando
Published: March 2023

Las transformaciones crean resistencias. Mupsicoterapeuta y terapeuta no verbal por terapeuta Benenzon; identidad sonora por identidad sensorial. Paciente por elotro, yo otro, nos otros. Los 9 principios de la etica del no verbal de la terapia Benenzon.
Pintura y Experimentación. Ruptura del espacio unitario.la experiencia neofigurativa.
Authors: Echeveste Susana
Published: March 2023

En las propuestas experimentales realizadas luego de las segundas vanguardias del s.XXse destacan las realizadas por los pintores de la Neofiguración argentina(1960/3). Tomando las obras de dos de ellos se informan y analizan los recursos pictóricos y extrapictóricos que conformanobras que apelan a la ruptura de la unidad del cuadro.Siendo los representantes de una corriente deconstructiva que se adelanta a las teorizaciones europeas,poniendo en obralos quiebres y fragmentos de una producción de tipicamente argentina.
Dimensões Modernas das Revistas Ilustradas: Curitiba, Iní­cio do Século Xx
Authors: Kaminski Rosane
Published: March 2023

O texto busca evidenciar a participação das imagens de revistas na elaboração de uma percepção estética ligada à vida moderna, em Curitiba no iní­cio do século XX. As revistas integraram e testemunharam uma transformação nos padrões de gosto e de comportamento cultural na cidade, num momento em que ocorria simultaneamente uma diminuição do número de revistas literárias, o fortalecimento da lógica publicitária associada à imprensa, e o surgimento de uma série de publicações ilustradas de humor e variedades sobre temas urbanos. Elas ocuparam um espaço pioneiro no que diz respeito à produção e fruição visual na cidade, contemporâneas que foram à constituição do meio artí­stico que recentemente vinha se formando na cidade. Neste texto, o caráter moderno das imagens publicadas nessas revistas é discutido a partir de três dimensões. A primeira refere-se ao fato de que as imagens e os conteúdos das revistas veiculavam e reforçavam esquemas de comportamento como a moda e os novos hábitos de lazer da vida urbana naquele momento. A segunda dimensão moderna da experiência perceptiva ia se constituindo a partir do próprio contato visual com as revistas, gerando novos esquemas de gosto pela assimilação de novos traçados, estilos e sutilezas visuais advindos tanto do projeto gráfico quanto das inovações nas técnicas de reprodução de cores e imagens. Já a terceira dimensão moderna refere-se ao caráter metalinguí­stico, enfatizado quando a imagem fala de si própria enquanto linguagem. Traço que marcou a produção de vários artistas de vanguarda noutros centros, a metalinguagem, em Curitiba, aparece antes nas imagens de revistas do que nas artes plásticas. Essas três dimensões coexistem e se sobrepõe, participando da edificação da modernidade visual em Curitiba.
Tecnologia, Arte e Entretenimento: A Obscuridade dos Artifí­cios para Estí­mulo do Progresso e do Consumo.
Authors: Melo Venise Paschoal de , Silveira Luciana Martha
Published: March 2023

A proposta deste artigo é gerar reflexões a respeito das relações entre os avanços tecnológicos e o uso do entretenimento, inseridos no contexto da arte contemporânea, como um modo de estimular determinadas ideologias e reforçar sistemas de controle sobre determinado público, visando estimular, principalmente a crença no progresso tecnológico. Apresentamos nosso argumento localizado em três momentos distintos: (1) Exposições Universais do século XIX; (2) as implicações sobre o contexto dos meios de comunicação e o consumo, especificamente sobre o cinema e a televisão, e (3) a problematização da arte no século XXI, quando inserido nos dos meios digitais.
O problema dos "novos museus", e nosso
Authors: Bruel Gemin Deborah Alice
Published: March 2023

O presente texto apresenta os problemas das exposições de arte contemporânea nos espaços museológicos e suas estratégias para popularizar a arte. Estratégias que podem se tornar artifí­cios de vulgarização da arte por não respeitar a capacidade perceptiva do observador e por aproximar-se cada vez mais da lógica dos grandes eventos culturais, como bens de consumo rápido e fácil. A partir de um trecho de texto de Otí­lia Arantes que reclama da "cultura de recolhimento como descartável" este artigo apresenta argumentos teóricos e exemplos práticos de trabalhos de arte que buscam discutir estes problemas.
Ruí­na Programada
Authors: Rayck Diego
Published: March 2023

Este artigo apresenta considerações acerca da obra Partially Buried Woodshed do artista Robert Smithson priorizando a atenção na relação entre desenho e ruí­na. Para isto analisa o processo de realização desta obra mencionando a idéia de entropia e a tendência a uma metodologia dialética que foram fundamentais ao artista. O entendimento de temporalidade com o qual Smithson trabalha relativiza o encadeamento causal e lhe permite elaborar a noção de "ruí­na em reverso" , posicionamento que estimula a refletir sobre a participação do desenho em seu processo de trabalho.
Favelas, Coroinhas e Vestí­gios da Floresta: Os Rumos da Pintura Abstrata no Paraná
Authors: Perigo Katiucya
Published: March 2023

Depois de alcançarem a consagração, alguns artistas tendem a realizar uma produção uniforme, sem grandes variações. Outros, no entanto, modificam sua produção ao bel prazer do grande público. Para a História da Arte, essas situações são apontadas frequentemente como "comuns" e, consequentemente, podem ser também observadas nas carreiras de artistas do Estado brasileiro do Paraná. Assim, depoimentos concedidos por três pintores regionais – Domí­cio Pedroso, Fernando Velloso e Érico da Silva – ilustram perfeitamente essa questão e contribuem para que acompanhemos suas trajetórias artí­sticas. As reflexões de Pierre Bourdieu e Sí­lvio Zamboni permitem elucidar as escolhas feitas por eles. Foi possí­vel verificar, por exemplo, a existência de dois tipos de consagração: a de mercado, ligada a um público mais amplo, de não iniciados, e a simbólica, aliada a um pequeno público seleto de especialistas. Deslocando-se para fora do eixo Rio-São Paulo, este estudo objetiva ainda contribuir com a compreensão que se tem da história da arte brasileira.
A densidade da solidão em "O melhor lance", de Giuseppe Tornatore
Authors: Da Rolt Clóvis
Published: March 2023

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Gordura, Gênero e Humor em Elisa Queiroz
Authors: Almeida de Mello Júlia , Cirillo José
Published: March 2023

O presente artigo realiza uma discussão da utilização do corpo gordo como imagem geradora nas obras de Elisa Queiroz (1970-2011) atrelada a questões concernentes ao gênero. A artista multifacetada possuí­a como peça chave da sua criação o bom humor. Apresentamos a sua trajetória trazendo alguns documentos de processo e várias obras até adentrarmos com maior atenção nas que foram feitas a partir de suportes comestí­veis ("Sirva-se" [2002], "Macarrão aos frutos do mar" [2003] e "Piquenique na relva com formigas" [2004]) e na videoarte "Free Williams" (2004). Os resultados revelam uma busca da artista em contestar a hegemonia e a sua arte se mostra como um forte recurso para isso.
Onde se Fundem os Mitos: Reflexões sobre a Obra de Leonor Botteri
Authors: Lacombe Feijó Pedro Paulo
Published: March 2023

Neste artigo, baseado em nuances do pensamento de autores ligados à filosofia de Arthur Schopenhauer, procurei construir um diálogo em torno da obra de Leonor Botteri que possa revelar indí­cios da possí­vel realidade transcendente da pintora e a natureza dos elementos responsáveis pelo espaço estético do universo botteriano. Descrevi as impressões que a obra de Botteri me desperta, particularmente as telas O vento da noite e Menina do campo, das quaisfiz uma leitura informal, não estilí­stica, e comentei as significativas coincidências compositivas, estruturais e simbólicas que existem entre elas. Sob meu ponto de vista, esses dois trabalhos se correspondem e se complementam e a pintura Menina do campo pode ser uma representação inconsciente da artista quando criança. Considero que a obra de Botteri requer uma abordagem especial, por isso, para nortear a forma de escrever, busquei alento em Gaston Bachelard, à procura de uma linguagem ao mesmo tempo crí­tica e poética.
MONTANDO CONFLITOS: Griffith na Biograph
Authors: Carvalho Barbosa Paulo Roberto de
Published: March 2023

Este artigo examina filmes escolhidos de David Wark Griffith em seu perí­odo Biograph. O texto recupera a estreia profissional de Griffith como ator de teatro e a sua posterior conversão à indústria do cinema. Descreve como realizou seus primeiros filmes, estruturando-os a partir de esquemas narrativos provenientes dos melodramas, gênero de longa tradição nos palcos. O exame desses filmes revela estruturas compostas de diferentes núcleos dramáticos, estabelecendo polarizações entre si. Polarizações que formam a base para o desenvolvimento de conflitos semanticamente operados pela montagem fí­lmica. É agenciando planos para criar relações de oposição, convergência e bifurcação nas tramas que Griffith tece as espirais dramáticas de suas narrativas, nem sempre terminadas em finais felizes. De uma tal montagem, não inteiramente clássica e tampouco "primitiva" , emerge o código moral do diretor sulista, conservador em muitos aspectos, mas também progressista em diversos outros.
NO HAY PUERTA DE ENTRADA: A recorrência como Poética de Criação
Authors: Santos Luis Henrique Larocca
Published: March 2023

Abordo aqui a recorrência da representação de assuntos relacionados à casa, como imagem e como conceito, ao longo de todo o meu processo de criação artí­stica. Procuro investigar como o retorno desses elementos contribui para a criação de uma narrativa e de que maneira o processo de acúmulo transforma o sentido de cada trabalho. Identifico em certas situações e imagens o conceito do "estranho" , de Sigmund Freud, e também algumas noções sobre o fantástico. Busco, por fim, relacionar o relato dos meus trabalhos à história das imagens e ao conceito de memória para o historiador Walter Benjamin, na medida em que opero a partir da justaposição e do anacronismo.
MOTION LAYERS: uma de muitas leituras possí­veis.
Authors: Oliveira Aline Rayane de Souza
Published: March 2023

O presente artigo consiste em um exercí­cio teórico que, aplicando o método de leitura da imagem estabelecido por Edmund Feldman, oferece possí­veis leituras para os murais do Motion Layers. O projeto vem sendo desenvolvido de maneira colaborativa desde 2009 pelos artistas Dimas, Artestenciva e Leandro Cí­nico, que contaram com ajuda de vários parceiros. As intervenções realizadas no Setor Histórico de Curitiba foram viabilizadas pela Lei de Incentivo à Cultura, na Modalidade de Mecenato Subsidiado, e busca estabelecer relações práticas e teóricas entre a técnica do estêncil e do cinema, levando a um permanente estado de observação, crí­tica e análise sobre a universalidade da cultura pop em uma perspectiva local.
The Relationship of Body Fat Ratio to Anthropometric and Metabolic Parameters in Normal Weight and Overweight Women; The Concept of Normal Weight Obesity
Authors: Kucukdag Huseyin Nejat , Sonmez Cemil Isik
Published: March 2023

Aim: We aimed to underline the importance of “normal weight obesity concept”, prevent the overlooking of Normal Weight Obesity patients, administrate their treatments and explain them the risks regarding their future lives along with making emphasis on the necessity of change in polyclinical practices about the subject.

Methods: We selected 200 female patients that were either normal or overweighted for this study. Patients have been divided into 4 groups with respect to their body mass index and body fat percent values. Differences of bioelectrical impedance analysis measurements between the groups, relation of the anthropometric and metabolic parameters and those parameters’ inner correlations have been inspected.

Results: Serum levels of fasting plasma glucose, insulin, HOMA-IR, total cholesterol, LDL, triglyceride and TSH were higher in “Normal Weight Obesity” subjects than “Normal Weight Lean” subjects. The mean LDL levels were found to be higher in the Normal Weight Obesity group compared to the other groups and it was found to be statistically significant. Body fat percent was positively correlated to insulin, HOMA-IR, visceral fat rating, waist – hip circumferences, waist to height – hip to height ratios.

Conclusion: The importance of measurement of body fat percent and not to overlook the Normal Weight Obesity patients should be emphasized once more. Therefore, in the primary care, during public screening and polyclinical assessment states, patients should be checked for obesity with not only measuring their weights and heights, but also detection of their detailed body composition.

Keywords: obesity, body fat distribution, waist to height ratio
Artificial neural network scheme to solve the hepatitis B virus model
Authors: Haider Qusain , Hassan Ali , Eldin Sayed M.
Published: March 2023

This article aims to describe the simulation studies of the hepatitis B virus non-linear system using supervised neural networks procedures supported by Levenberg-Marquardt back propagation methodology. The proposed strategy has five distinct quantities: susceptible X(t), symptomatic infections Y(t), chronic infections W(t), recovered population R(t), and a population that has received vaccinations Z(t). The reference data set for all three distinct cases has been obtained utilizing the ND-Solver and Adams method in Mathematica software. The outcomes have been validated with performance plots for all cases. To check the accuracy and effectiveness of proposed methodology mean square error has are presented. State transition, and regression plots are illustrated to elaborated the testing, training, and validation methodology. Additionally, absolute errors for different components of hepatitis B virus model are demonstrated to depict the error occurring during distinct cases. Whereas the data assigned to training is 81%, and 9% for each testing and validation. The mean square error for all three cases is 10−12 this show the accuracy and correctness of proposed methodology.
Comprehensive ex vivo and in vivo preclinical evaluation of novel chemo enzymatic decellularized peripheral nerve allografts
Authors: García-García Óscar Darío , El Soury Marwa , Campos Fernando , Sánchez-Porras David , Geuna Stefano , Alaminos Miguel , Gambarotta Giovanna , Chato-Astrain Jesús , Raimondo Stefania , Carriel Víctor
Published: March 2023

As a reliable alternative to autografts, decellularized peripheral nerve allografts (DPNAs) should mimic the complex microstructure of native nerves and be immunogenically compatible. Nevertheless, there is a current lack of decellularization methods able to remove peripheral nerve cells without significantly altering the nerve extracellular matrix (ECM). The aims of this study are firstly to characterize ex vivo, in a histological, biochemical, biomechanical and ultrastructural way, three novel chemical-enzymatic decellularization protocols (P1, P2 and P3) in rat sciatic nerves and compared with the Sondell classic decellularization method and then, to select the most promising DPNAs to be tested in vivo. All the DPNAs generated present an efficient removal of the cellular material and myelin, while preserving the laminin and collagen network of the ECM (except P3) and were free from any significant alterations in the biomechanical parameters and biocompatibility properties. Then, P1 and P2 were selected to evaluate their regenerative effectivity and were compared with Sondell and autograft techniques in an in vivo model of sciatic defect with a 10-mm gap, after 15 weeks of follow-up. All study groups showed a partial motor and sensory recovery that were in correlation with the histological, histomorphometrical and ultrastructural analyses of nerve regeneration, being P2 the protocol showing the most similar results to the autograft control group.
Isolation and screening of a chitin deacetylase producing Bacillus cereus and its potential for chitosan preparation
Authors: Zhang Yingying , Luo Xi , Yin Longfei , Yin Fengwei , Zheng Weilong , Fu Yongqian
Published: March 2023

Chitosan is a biopolymer material extracted from marine biomass waste such as shrimp and crab shells, which has good biocompatibility and degradability with great potential for application in the field of wastewater treatment and soil remediation. The higher the degree of deacetylation (DD), the better the adsorption performance of chitosan. Chitin deacetylase (CDA) can specifically catalyze the deacetylate of chitin in a green reaction that is environmentally friendly. However, the scarcity of high yielding chitin deacetylase strains has been regarded as the technical bottleneck of chitosan green production. Here, we screened a natural chitin degrading bacterium from coastal mud and identified it as Bacillus cereus ZWT-08 by re-screening the chitin deacetylase activity and degree of deacetylation values. By optimizing the medium conditions and enzyme production process, ZWT-08 was cultured in fermentation medium with 1% (m/V) glucose and yeast extract at pH 6.0, 37°C, and a stirring speed of 180 r/min. After fermenting in 5 L fermenter for 48 h, the deacetylation activity of the supernatant reached 613.25 U/mL. Electron microscopic examination of the chitin substrate in the fermentation medium revealed a marshmallow-like fluffy texture on its structural surface. Meanwhile, 89.29% of the acetyl groups in this chitin substrate were removed by enzymatic digestion of chitin deacetylase produced by ZWT-08, resulting in the preparation of chitosan a degree of deacetylation higher than 90%. As an effective strain for chitosan production, Bacillus cereus ZWT-08 plays a positive role in the bioconversion of chitin and the upgrading of the chitosan industry.
Cardiopulmonary bypass for total aortic arch replacement surgery: A review of three techniques
Authors: Cui Ying , Liu Xinhao , Xiong Jiyue , Tan Zhaoxia , Du Lei , Lin Jing
Published: March 2023

One treatment for acute type A aortic dissection is to replace the ascending aorta and aortic arch with a graft during circulatory arrest of the lower body, but this is associated with high mortality and morbidity. Maintaining the balance between oxygen supply and demand during circulatory arrest is the key to reducing morbidity and is the primary challenge during body perfusion. The aim of this review is to summarize current knowledge of body perfusion techniques and to predict future development of this field. We present three perfusion techniques based on deep hypothermic circulatory arrest (DHCA): DHCA alone, DHCA with selective cerebral perfusion, and DHCA with total body perfusion. DHCA was first developed to provide a clear surgical field, but it may contribute to stroke in 4%–15% of patients. Antegrade or retrograde cerebral perfusion can provide blood flow for the brain during circulatory arrest, and it is associated with much lower stroke incidence of 3%–9%. Antegrade cerebral perfusion may be better than retrograde perfusion during longer arrest. In theory, blood flow can be provided to all vital organs through total body perfusion, which can be implemented via either arterial or venous systems, or by combining retrograde inferior vena caval perfusion with antegrade cerebral perfusion. However, whether total body perfusion is better than other techniques require further investigation in large, multicenter studies. Current techniques for perfusion during circulatory arrest remain imperfect, and a technique that effectively perfuses the upper and lower body effectively during circulatory arrest is missing. Total body perfusion should be systematically compared against selective cerebral perfusion for improving outcomes after circulatory arrest.
Multimodality imaging to identify lipid-rich coronary plaques and predict periprocedural myocardial injury: Association between near-infrared spectroscopy and coronary computed tomography angiography
Authors: Ota Hideaki , Matsuo Hitoshi , Imai Shunsuke , Nakashima Yuki , Kawase Yoshiaki , Okubo Munenori , Takahashi Hiroshi , Kawai Hideki , Sobue Yoshihiro , Kawasaki Masanori , Kondo Takeshi , Muramatsu Takashi , Izawa Hideo
Published: March 2023

Background

This study compares the efficacy of coronary computed tomography angiography (CCTA) and near-infrared spectroscopy intravascular ultrasound (NIRS–IVUS) in patients with significant coronary stenosis for predicting periprocedural myocardial injury during percutaneous coronary intervention (PCI).

Methods

We prospectively enrolled 107 patients who underwent CCTA before PCI and performed NIRS–IVUS during PCI. Based on the maximal lipid core burden index for any 4-mm longitudinal segments (maxLCBI4mm) in the culprit lesion, we divided the patients into two groups: lipid-rich plaque (LRP) group (maxLCBI4mm ≥ 400; n = 48) and no-LRP group (maxLCBI4mm < 400; n = 59). Periprocedural myocardial injury was a postprocedural cardiac troponin T (cTnT) elevation of ≥5 times the upper limit of normal.

Results

The LRP group had a significantly higher cTnT (p = 0.026), lower CT density (p < 0.001), larger percentage atheroma volume (PAV) by NIRS–IVUS (p = 0.036), and larger remodeling index measured by both CCTA (p = 0.020) and NIRS–IVUS (p < 0.001). A significant negative linear correlation was found between maxLCBI4mm and CT density (rho = −0.552, p < 0.001). Multivariable logistic regression analysis identified maxLCBI4mm [odds ratio (OR): 1.006, p = 0.003] and PAV (OR: 1.125, p = 0.014) as independent predictors of periprocedural myocardial injury, while CT density was not an independent predictor (OR: 0.991, p = 0.22).

Conclusion

CCTA and NIRS–IVUS correlated well to identify LRP in culprit lesions. However, NIRS–IVUS was more competent in predicting the risk of periprocedural myocardial injury.
Injury to the tunica media initiates atherogenesis in the presence of hyperlipidemia
Authors: Belhoul-Fakir Hanane , Wu Jiansha , Yeow Yen L. , Musk Gabrielle C. , Kershaw Helen , Ingley Evan , Zhao Bichen Sophie , Reid Christopher M. , Lagat Christopher , Evans Brian , Thompson Peter L. , Brown Michael L. , Hamzah Juliana , Jansen Shirley
Published: March 2023

Background and aims

Fatty streaks initiating the formation of atheromatous plaque appear in the tunica intima. The tunica media is not known to be a nidus for lipid accumulation initiating atherogenesis. We assessed changes to the tunica media in response to a micro-injury produced in the pig aorta. In addition, we assessed human carotid endarterectomy plaques for indication of atheroma initiation in the tunica media.

Methods

Three healthy landrace female pigs underwent laparotomy to inject autologous blood and create micro-hematomas at 6 sites within the tunica media of the infrarenal abdominal aorta. These pigs were fed a high-fat diet (HFD) for 4–12 weeks. Post-mortem aortas from all pigs, including a control group of healthy pigs, were serially stained to detect lipid deposits, vasa vasora (VV), immune cell infiltration and inflammatory markers, as well as changes to the vascular smooth muscle cell (vSMC) compartment. Moreover, 25 human carotid endarterectomy (CEA) specimens were evaluated for their lipid composition in the tunica media and intima.

Results

High lipid clusters, VV density, and immune cell infiltrates were consistently observed at 5 out of 6 injection sites under prolonged hyperlipidemia. The hyperlipidemic diet also affected the vSMC compartment in the tunica media adjacent to the tunica adventitia, which correlated with VV invasion and immune cell infiltration. Analysis of human carotid specimens post-CEA indicated that 32% of patients had significantly greater atheroma in the tunica media than in the arterial intima.

Conclusion

The arterial intima is not the only site for atherosclerosis initiation. We show that injury to the media can trigger atherogenesis.
Mechanism, risk, and solution of cultivated land reversion to mountains and abandonment in China
Authors: Xie Zhen , Fan Shenglong , Du Shaorong , Zheng Yong , Li Chao
Published: March 2023

The cultivated land requisition-compensation balance (CLRB) system in China has been designed to defend cultivated land resources and grain production functions. Nevertheless, since the addition of a new policy, namely the linkage of increase and decrease (LID) of urban and rural construction land in 2008, a mass of cultivated land has been returning to mountains, sometimes resulting in abandonment. The county of Wannian was investigated from the microcosmic perspective, and we attempted to analyze the causes and risks employing the boosted regression trees (BRT) model and the grain productive capacity assessment model. The results indicate that (1) The compensatory cultivated land (CCL) has shifted uphill, with considerable fragmentation, from 2010 to 2020, and the abandonment rate reached 14.77%. (2) The factors of site condition, including elevation, patch area, and continuity of cultivated land, as well as a series of combinations, can explain the causes of abandonment. (3) The abandonment of these cultivated land areas eventually resulted in the risk of 297.48 t grain production capacity loss. The reason for the return of cultivated land to the mountains and its subsequent abandonment is the lack of consideration for the coupling relationship among site condition, use state, and function requirement, resulting in a spatial mismatch. Based on the findings, we propose a solution of the natural resources requisition-compensation balance (NRRB). To make up for the loss and reduce the risk, a spatial replacement was taken between the abandoned CCL in uphill and cultivable and available forest land (CAFL) in submontane areas CCL, optimizing the spatial pattern of land use toward Von Thunen’s agricultural circle.
Cost-effectiveness of a bone substitute delivering gentamicin in the treatment of chronic osteomyelitis of long bones: Protocol for the CONVICTION randomized multicenter study
Authors: Serrier Hassan , Huot Laure , Brosset Sophie , Batailler Cécile , Ferry Tristan
Published: March 2023

Introduction

Chronic osteomyelitis is a serious osteoarticular infection that most often occurs in the long bones, responsible for significant morbidity with the risk of fracture and amputation. Despite advances in both antibiotics and surgical treatment, the probability of recurrence of infection remains at around 20%. Cerament-G (BONESUPPORT AB, Sweden) is a synthetic bone substitute that fills the bone void left by surgery, prevents infection and promotes bone regeneration within this space. Cerament-G also provides the local delivery of high doses of gentamicin over several weeks. Two prospective observational studies described a number of infectious recurrences of 4 and 5% after the use of Cerament-G. Although available in France, Cerament-G is currently not reimbursed and its high cost constitutes a barrier to its use. We hypothesize that the use of Cerament-G will lead to fewer costs to the collectivity while improving patient utility and, as an innovative strategy, will be superior to standard of care on recurrence of infection.

Methods and analysis

The Conviction Study is a prospective, multicenter, randomized, single blind study conducted in 14 French Reference Centers for Complex Osteoarticular infections. The main objective is to evaluate the cost-effectiveness of using Cerament-G in the treatment of chronic long bone osteomyelitis by comparing this innovative strategy to standard of care. A cost-utility analysis from the collective perspective will be conducted over a 24-month time horizon after the initial surgery. The outcome for the main medico-economic evaluation will be Quality Adjusted Life Years (QALYs).

Discussion

The study is being conducted throughout the CRIOAc network in France, in referral centers for the management of complex infections which will facilitate patient recruitment. This study has several limitations: the investigators have to be trained to handle the device, and it was impossible to blind the surgeon.

Conclusion

If the use of Cerament-G is demonstrated to be superior to leaving the dead space empty during surgery for patients with stage III chronic long bone osteomyelitis, its use will be recommended to improve the prognosis of such patients, and this device may eventually qualify for reimbursement through the French Health Insurance scheme.

Ethics and dissemination

This protocol received authorization from the Ethics Committee CPP Sud Méditerranée V on April 27, 2021 (21.03.10.77652) and the French National Agency for Medicines and Health Products on May 6, 2021 (2020-A02299-30). Results will be disseminated to the scientific community through congresses and publication in peer-reviewed journals.
The relationship between triglyceride, cholesterol and lipoprotein levels, and immune responses to hepatitis B vaccine
Authors: Guo Dan , Dai Jiazhen , Ju Rong , Zhou Qifan , Wang Nenghuan , Wu Chunhua , Tao Hui , Jing Hui , Zhu Chen , Mao Jinxian , Xu Jiayan
Published: March 2023

Cholesterol homeostasis disorder and hypertriglyceridemia, as common metabolic conditions, have rarely been reported to affect the immune responses to the hepatitis B vaccine. Our study found that higher high-density lipoprotein (HDL) level showed a significant relationship with positive anti-HBs results (cOR = 1.479, 95% CI: 1.150, 1.901, p = 0.002; aOR = 1.304, 95% CI: 1.006, 1.691, p = 0.045), especially in individuals aged 18- to 40-year-old, female, smoking more than 100 cigarettes in life, and drinking more than 12 times every year. Lower low-density lipoprotein (LDL) level was associated with a negative anti-HBs result among participants aged 18- to 40-year-old, and participants who were obese. Higher level of HDL and lower level of LDL may be protective factors of better immune effect of hepatitis B vaccine. More research should be conducted to investigate the influence of the cholesterol level on the immune responses to the hepatitis B vaccine, and more in-depth research should be performed to uncover the mechanism.
Association between red blood cell distribution width and all-cause mortality in unselected critically ill patients: Analysis of the MIMIC-III database
Authors: Peng Shixuan , Li Wenxuan , Ke Weiqi
Published: March 2023

Background

Although red cell distribution width (RDW) is widely observed in clinical practice, only a few studies have looked at all-cause mortality in unselected critically ill patients, and there are even fewer studies on long-term mortality. The goal of our study was to explore the prediction and inference of mortality in unselected critically ill patients by assessing RDW levels.

Methods

We obtained demographic information, laboratory results, prevalence data, and vital signs from the unselected critically ill patients using the publicly available MIMIC-III database. We then used this information to analyze the association between baseline RDW levels and unselected critically ill patients using Cox proportional risk analysis, smoothed curve fitting, subgroup analysis, and Kaplan–Meier survival curves for short, intermediate, and long-term all-cause mortality in unselected critically ill patients.

Results

A total of 26,818 patients were included in our study for the final data analysis after screening in accordance with acceptable conditions. Our study investigated the relationship between RDW levels and all-cause mortality in a non-selected population by a smoothed curve fit plots and COX proportional risk regression models integrating cubic spline functions reported results about a non-linear relationship. In the fully adjusted model, we found that RDW values were positively associated with 30-day, 90-day, 365-day, and 4-year all-cause mortality in 26,818 non-selected adult patients with HRs of 1.10 95%CIs (1.08, 1.12); 1.11 95%CIs (1.10, 1.13); 1.13 95%CIs (1.12, 1.14); 1.13 95%CIs (1.12, 1.14).

Conclusion

In unselected critically ill patients, RDW levels were positively associated with all-cause mortality, with elevated RDW levels increasing all-cause mortality.
A prognostic assessment predicated by blood culture-based bacteria clustering from real-world evidence: Novel strategies and perspectives on prevention and management of sepsis
Authors: Xu Shaokang , Cai Jizhen , Doomi Ahmed , Shi Jian
Published: March 2023

Sepsis, a syndrome with disturbed host response to severe infection, is a critical health problem worldwide. It is urged to develop and update novel therapeutic strategies for improving the outcome of sepsis. In this study, we demonstrated that different bacteria clustering in sepsis patients may generate differences of prognosis results. We extracted all the sepsis patients from Medical Information Mart for Intensive Care IV 2.0 (MIMIC-IV 2.0) critical care data set according to certain standards and clinical score, a total of 2,339 patients were included in our study. Then we used multiple data analytics and machine learning methods to make all data deeply analyzed and elucidated. The results showed that the types of bacteria infected by patients with different ages, sex and race are different, the types of bacteria infected by patients with different SIRS values and GCS scores of the first day are different, and the severity of patients with different clusters is different, and most importantly, the survival rate of patients with different clusters also has this significant difference. We concluded prognostic assessment predicated by bacteria clustering might be a relatively potentially novel strategies and perspectives on prevention and management for sepsis in the future.
Women's reproductive traits and cerebral small-vessel disease: A two-sample Mendelian randomization study
Authors: Wang Zhenqian , Lu Jiawen , Weng Weipin , Zhang Jie
Published: March 2023

Background

Observational studies have suggested that women's reproductive factors (age at menarche (AAM), age at first birth (AFB), age at first sexual intercourse (AFS), age at natural menopause (ANM), and pregnancy loss) may influence the risk of cerebral small-vessel disease (CSVD) although the causality remains unclear.

Methods

We conducted two-sample univariable Mendelian randomization (UVMR) and multivariable MR (MVMR) to simultaneously investigate the causal relationships between five women's reproductive traits and CSVD clinical [intracerebral hemorrhage (ICH) by location or small-vessel ischemic stroke (SVS)] and subclinical measures [white matter hyperintensities (WMH), fractional anisotropy (FA), and mean diffusivity (MD)], utilizing data from large-scale genome-wide association studies of European ancestry. For both UVMR and MVMR, the inverse-variance-weighted (IVW) estimates were reported as the main results. The MR-Egger, weighted median, generalized summary-data-based MR (GSMR), and MR-pleiotropy residual sum and outlier (MR-PRESSO) methods for UVMR and MVMR-Egger, and the MVMR-robust methods for MVMR were used as sensitivity analyses. Sex-combined instruments for AFS and AFB were used to assess the impact of sex instrumental heterogeneity. Positive control analysis was implemented to measure the efficacy of selected genetic instruments.

Results

We found no evidence to support causal associations between genetic liability for women's reproductive factors and the risk of CSVD in UVMR (all P-values > 0.05). Using MVMR, the results were consistent with the findings of UVMR after accounting for body mass index and educational attainment (all P-values > 0.05). Sensitivity analyses also provided consistent results. The putative positive causality was observed between AAM, ANM, and ovarian cancer, ensuring the efficacy of selected genetic instruments.

Conclusion

Our findings do not convincingly support a causal effect of women's reproductive factors on CSVD. Future studies are warranted to investigate specific estrogen-related physiological changes in women, which may inform current researchers on the causal mechanisms involved in cerebral small-vessel disease progression.
Evaluation of a clinical method for selective electrode deactivation in cochlear implant programming
Authors: Warren Sarah E. , Atcherson Samuel R.
Published: March 2023

Background

Cochlear implants are a neural prosthesis used to restore the perception of hearing in individuals with severe-to-profound hearing loss by stimulating the auditory nerve with electrical current through a surgically implanted electrode array. The integrity of the interface between the implanted electrode array and the auditory nerve contributes to the variability in outcomes experienced by cochlear implant users. Strategies to identify and eliminate poorly encoding electrodes have been found to be effective in improving outcomes with the device, but application is limited in a clinical setting.

Objective

The purpose of this study was to evaluate a clinical method used to identify and selectively deactivate cochlear implants (CI) electrodes related to poor electrode-neural interface.

Methods

Thirteen adult CI users participated in a pitch ranking task to identify indiscriminate electrode pairs. Electrodes associated with indiscriminate pairs were selectively deactivated, creating an individualized experimental program. Speech perception was evaluated in the baseline condition and with the experimental program before and after an acclimation period. Participant preference responses were recorded at each visit.

Results

Statistically significant improvements using the experimental program were found in at least one measure of speech perception at the individual level in four out of 13 participants when tested before acclimation. Following an acclimation period, ten out of 13 participants demonstrated statistically significant improvements in at least one measure of speech perception. Statistically significant improvements were found with the experimental program at the group level for both monosyllabic words (p = 0.006) and sentences in noise (p = 0.020). Additionally, ten participants preferred the experimental program prior to the acclimation period and eleven preferred the experimental program following the acclimation period.

Conclusion

Results from this study suggest that electrode deactivation may yield improvement in speech perception following an acclimation period. A majority of CI users in our study reported a preference for the experimental program. This method proved to be a suitable clinical strategy for identifying and deactivating poorly encoding electrodes in adult CI users.
Optimizing culturing conditions in patient derived 3D primary slice cultures of head and neck cancer
Authors: Greier Maria do Carmo , Runge Annette , Dudas Jozsef , Carpentari Lukas , Schartinger Volker Hans , Randhawa Avneet , Mayr Melissa , Petersson Monika , Riechelmann Herbert
Published: March 2023

Background

Three-dimensional primary slice cultures (SC) of head and neck squamous cell carcinomas (HNC) are realistic preclinical models. Until now, preserving structure and viability ex vivo for several days has been difficult. The aim of this study was to optimize cultivation conditions for HNC SC and analyze the added effects of platelet rich fibrin (PRF) on these conditions.

Methods

SC were prepared from the tumor biopsies of 9 HNC patients. Cultures were incubated for 1 and 7 days in three different media- Keratinocyte serum-free medium (SFM), RPMI-1640i, and 1:1 mix of both, with and without addition of PRF. After culturing, SC were fixated, embedded, and stained with Hematoxylin-Eosin (HE) and cleaved caspase-3. In addition, triple immune fluorescence staining for cytokeratin, vimentin and CD45 was performed. Outcome parameters were cell count and cell density, viability and apoptosis, SC total area and proportions of keratinocytes, mesenchymal and immune cells. The effects of culture time, medium, and addition of PRF were calculated in an SPSS generalized linear model and using the Wald Chi-Squared test.

Results

Ninety-four slice cultures were analyzed. Viability remained stable for 7 days in culture. After addition of PRF, cell viability increased (p=0.05). SC total area decreased (0.44 ± 0.04 mm2 on day 1 (95% CI: 0.35 to 0.56) to 0.29 ± 0.03 mm2 on day 7 (95% CI: 0.22 to 0.36), but cell density and cell proportions remained stable. Differences in cultivation media had no significant impact on outcome parameters.

Conclusion

HNC SC can be preserved for up to 7 days using the tested cultivation media. Cell viability was best preserved with addition of PRF. HNC SC are a versatile experimental tool to study physiology and drug actions. Autologous PRF can help simulate realistic conditions in vitro.
Amphipathic barbiturates as marine product mimics with cytolytic and immunogenic effects on head and neck squamous cell carcinoma cell lines
Authors: von Hofsten Susannah , Langer Manuel K. , Korelin Katja , Magnussen Synnøve , Ausbacher Dominik , Anderssen Trude , Salo Tuula , Strøm Morten B. , Bayer Annette , Al-Samadi Ahmed , Berge Gerd
Published: March 2023

The incidence of head and neck squamous cell carcinoma (HNSCC) is increasing and the conventional treatments for this form of cancer can be tough. Despite the success of existing immunotherapies in some HNSCC patients, many do not respond to this type of treatment. Thus, the development of novel anti-cancer therapies should be prioritized. In the current study, the anticancer activity of a panel of novel compounds, herein termed marine product mimics (MPMs), against HNSCC cell lines is explored. The previously reported compound MPM-1, which is structurally related to the novel MPMs, was shown to have promising effects on the HNSCC cell line HSC-3. The results from the current study indicate that the novel MPMs are more potent than MPM-1 but cause a similar type of cell death. The results indicated that the MPMs must cross through the cell membrane to exert their action and that they are lysosomotropic. Further experiments showed that some of the MPMs could induce phosphorylation of eukaryotic initiation factor 2α (eIF2α) in HSC-3 and UT-SCC-24A cells, which indicates that they can activate the integrated stress response that is strongly associated with immunogenic cell death. Cell surface expression of calreticulin and release of HMGB1 and ATP, which are all hallmarks of immunogenic cell death, was also demonstrated in HSC-3 and UT-SCC-24A cells treated with MPMs. This suggests that the MPMs are interesting candidates for future HNSCC cancer therapies.
Fragmentation of the 4He2 dimer with the emission of three or four electrons by fast highly charged projectiles
Authors: Najjari B. , Zhang S. F. , Ma X. , Voitkiv A. B.
Published: March 2023

We investigate the fragmentation of the helium dimer, 4He2, into He2+ + He+ and He2+ + He2+ ions in collisions with fast highly charged projectiles. We discuss the main physical mechanisms driving these processes. We explore the energy and angular distributions of the ionic fragments produced during collisions of the dimer with 1 GeV/u U92+ and 11.37 MeV/u S14+ projectiles and also present the total fragmentation cross-sections. According to our results, the fragmentation in these collisions is fully dominated by the direct removal of three or four electrons from the dimer by the projectile in a single collision. Our results also suggest that the total fragmentation cross-sections depend on the binding energy IHe2 of the dimer, being roughly proportional to IHe2.