bluuebunny/crossref_metadata_2025 huggingface dataset:
Differential contributions of distinct free radical peroxidation mechanisms to the induction of ferroptosis
Authors: Do Quynh , Zhang Rutan , Hooper Gavin , Xu Libin
Published: December 2022

Ferroptosis is a form of regulated cell death driven by lipid peroxidation of polyunsaturated fatty acids (PUFAs). Lipid peroxidation can propagate through either hydrogen-atom transfer (HAT) or peroxyl radical addition (PRA) mechanism. However, the contribution of the PRA mechanism to the induction of ferroptosis has not been studied. In this study, we aim to elucidate the relationship between the reactivity and mechanisms of lipid peroxidation and ferroptosis induction. We found that while some peroxidation-reactive lipids, such as 7-dehydrocholesterol, vitamins D3 and A, and coenzyme Q10, suppress ferroptosis, both nonconjugated and conjugated PUFAs enhanced cell death induced by RSL3, a ferroptosis inducer. Importantly, we found that conjugated polyunsaturated fatty acids (PUFAs), including conjugated linolenic acid (CLA 18:3) and conjugated linoleic acid (CLA 18:2) can induce or potentiate ferroptosis much more potently than nonconjugated PUFAs. We next sought to elucidate the mechanism underlying the different ferroptosis-inducing potency of conjugated and nonconjugated PUFAs. Lipidomics revealed that conjugated and nonconjugated PUFAs are incorporated into distinct cellular lipid species. The different peroxidation mechanisms predict the formation of higher levels of reactive electrophilic aldehydes from conjugated PUFAs than nonconjugated PUFAs, which was confirmed by aldehyde-trapping and mass spectrometry. RNA sequencing revealed that protein processing in the endoplasmic reticulum and proteasome are among the most significantly upregulated pathways in cells treated with CLA 18:3, suggesting increased ER stress and activation of unfolded protein response. Significantly, using click chemistry, we observed increased protein adduction by oxidized lipids in cells treated with an alkynylated CLA 18:2 probe. These results suggest that protein damage by lipid electrophiles is a key step in ferroptosis.
Using physical property surrogate models to perform multi-fidelity global optimization of force field parameters
Authors: Madin Owen , Shirts Michael
Published: December 2022

Dispersion-repulsion interactions, commonly represented in atomistic force fields by the Lennard-Jones (LJ) potential, play an important role in the accuracy of molecular simulations. Training the force field parameters used in the LJ potential is challenging, generally requiring adjustment based on simulations of macroscopic physical properties. The computational expense of these simulations limits the size of training data set and number of optimization steps that can be taken, often requiring modelers to perform optimizations within a local parameter region. To allow for global LJ parameter optimization against large training sets, we introduce a multi-fidelity optimization technique which uses Gaussian process surrogate modeling to build inexpensive models of physical properties as a function of LJ parameters. This allows for fast evaluation of objective functions, greatly accelerating searches over parameter space. We use an iterative framework which performs global optimization at the surrogate level, followed by validation at the simulation level and surrogate refinement. Using this technique on two previously studied training sets, containing up to 195 physical property targets, we refit a subset of the LJ parameters for the OpenFF 1.0.0 ``Parsley'' force field. We demonstrate that this multi-fidelity technique can find improved parameter sets compared to a purely simulation-based optimization by searching more broadly and escaping local minima. In most cases, these parameter sets are transferable to other similar molecules in a test set. This multi-fidelity technique provides a platform for fast optimization against physical properties that can be refined and applied in multiple ways to the development of molecular models.
Photocatalytic Cross-Pinacol Coupling
Authors: Okumura Shintaro , Takahashi Teruki , Torii Kaoru , Uozumi Yasuhiro
Published: December 2022

Cross-pinacol coupling of two different carbonyl compounds was achieved through successive one-electron transfer processes under photocatalytic conditions. In this process, an umpoled anionic carbinol synthon was generated in situ to react nucleophilically with a second electrophilic carbonyl compound. A wide variety of aromatic and aliphatic aldehydes and ketones were tolerated to afford the corresponding unsymmetric vicinal 1,2-diols.
Unexpected quasi-axial conformer in thermally activated delayed fluorescence DMAC-TRZ. Demonstrating how to turn green OLEDs into blue.
Authors: Stavrou Kleitos , Franca Larissa Gomes , Böhmer Tobias , Duben Luka , Marian Christel , Monkman Andrew
Published: December 2022

Although there have been several studies on the mechanism of thermally activated delayed fluorescence (TADF), providing useful models to understand the behaviour of TADF molecules, some photophysical features cannot be explained. Here, we investigate the hidden phenomena taking place in the very well-known TADF emitter, DMAC-TRZ. A molecule that, based on its structure, was considered not to fulfil the required criteria to have more than one ground and excited state conformation. However, based on experimental and computational studies, we have found two conformers, a quasi-axial (QA) and a quasi-equatorial (QE), and explored the effect of their co-existence on both optical and electrical excitation. Although the relative population of the QA conformer appears to be small, its effect is disproportionate because it has high local excited state character. The energy transfer efficiency from the QA to the QE conformer is shown to be high, even at low concentrations, and changes depending on the hosting environment. The currently known triplet energy of DMAC-TRZ quoted from experiment is shown to originate from the QA conformer, completely changing the understanding we have so far for this donor-acceptor molecule. The contribution of the QA conformer in devices has been explored and its presence explains the good performance of the material in neat emissive layer devices. Moreover, hyperfluorescnece (HF) devices, using v-DABNA as the terminal emitter show direct energy transfer from the QA conformer to v-DABNA, explaining the relative improved Förster resonance energy transfer (FRET) efficiency compared to similar HF systems. Using this approach, we demonstrate highly efficient organic light emitting diodes (OLEDs) were green light (TADF only devices) is converted to blue light (HF devices) with the maximum external quantum efficiency remaining high and close to 30%.
A Study of [2+2] Cycloaddition–Retroelectrocyclization in Water: Observation of Substrate-driven Transient Nanoreactor Induced New Reactivity
Authors: K. M Neethu , Nag Kritika , Hassan Dar Arif , Bajaj Ashima , Arya Gopal S. , Gowri Vijayendran , Nagpure Mithilesh , Sartaliya Shaifali , Sharma Raina , Kumar Solanki Arun , Ehesan Ali Md. , Muthukrishnan Azhagumuthu , Jayamurugan Govindasamy
Published: December 2022

Organic solvents limit [2+2] cycloaddition-retroelectrocyclization (CA–RE) in biological fields. We examined the formation of 1,1,4,4-tetracyanobuta-1,3-dienes (TCBDs) through CA–RE reactions and their unusual reactivity to produce N-heterocyclic compounds when surfactant nature and concentrations were varied in the aqueous phase. An environment in which transient self-assembly (vesicles) was induced by substrate and surfactant molecules initiated new reactivity through H2O addition on the TCBD generating enol form of the intermediate which results in the formation of the 6,6-dicyano-heteropentafulvene (amidofulvene) compound while lamellar sheets at higher concentrations favored TCBD generation. Interestingly, the amidofulvene underwent a clean transformation to 6-membered-heterocycles via keto-enol tautomerism mediated by a polar aprotic solvent which resembles cardiotonic drugs (milrinone, amrinone), opening up a new avenue for drug discovery. Unlike organic solvent-mediated CA–RE reactions, the present nanoreactor-mediated approach enabled the selective production of TCBDs as well as new heterocycles using H2O as a green solvent. Besides the widely explored organic electronics/materials, we believe that this study would help overcome the long-standing limitation of CA–RE reaction applicability in biological fields.
Paramount Definition of Reference Interval for Biochemistry Parameters in Indian Sub-Population: A Healthy Adult Population Based Cross Sectional Study
Authors: Babu SV Suresh , S Narayana , HR Suma , Shenoy R Rajesh
Published: December 2022

Background Biological reference intervals BRI of most of the laboratory parameters are significantly influenced by lifestyle and need to be reviewed periodically.Objectives To determine the reference intervals of common clinical-chemistry parameters such as fasting glucose urea creatinine total cholesterol triglycerides HDL- C Direct LDL-C Direct VLDL-C uric acid total protein albumin T. Bilirubin D. Bilirubin ALT AST g - GT ALP for the healthy adult population and to validate the newly established BRI for national applicability. Methodology A cross sectional observational study was conducted including 253 subjects with a mean age of 38.5plusmn8.7 years belonging to four geographical regions such as Kerala Karnataka Andhra Pradesh and Tamil Nadu. The analytes were measured using standard methods.Results Some of the reference intervals of common clinical chemistry parameters in healthy adults of Indian sub-population were lower than the real-world reference intervals however they were within the range. The important parameters such as blood urea creatinine uric acid total cholesterol total bilirubin and g- GT values were significantly lower than the mean reference interval by 35 30 16 9 20 and 57 respectively. However they were within the range while LDL- Cholesterol level was 37 above the mean reference limit. Rest of the parameters were harmonized with the reference interval.Conclusion The BRI were established in this study by constituting apparently healthy South Indian population. It may be a stepping platform and practical role in the determination of BRI of clinical-chemistry analytes in the future for Pan India.nbsp
Immunomodulatory Activity of Methanolic and Aqueous Extracts of Whole Plant of Achyranthes Aspera Linn. in Swiss Albino Mice and Wistar Albino Rats
Authors: Kolli Shweta , Nitin M , Inamdar Shivkumar , Ashok kumar Dastapur
Published: December 2022

In the traditional system of medicine of Ayurveda the medicinal plants that are known to modulate the immune response fall under the concept of rasayana. These plants also have been attributed with anabolic antistress adaptogenic nootropic antioxidant and anti-aging properties. A good immunomodulatory drug can be a breakthrough in the treatment of diseases where an abnormal immune response is the pathological cause such as cancer autoimmune diseases and natural or acquired immune deficiency syndromes. The aim of the present study was to investigate the immunomodulatory activity of methanolic and aqueous extracts of the whole plant of Achyranthes aspera Linn. in Swiss Albino mice and Wistar Albino rats. The first model was cyclophosphamide induced myelosuppression where cyclophosphamide was used to suppress the immune system in Swiss Albino mice and evaluated the plant extracts in reversal of myelosuppression by analyzing various hematological parameters. In the second model involving Arthus type immediate hypersensitivity the plant extracts were evaluated for protection from Arthus type immediate hypersensitivity in Wistar Albino rats. The potency of AEAA aqueous extract of Achyranthes aspera and MEAA methanolic extract of Achyranthes aspera were compared. Both AEAA and MEAA have shown significant immunomodulatory activity in both the cases. However MEAA was found to have higher potency plt 0.01 compared to AEAA plt 0.05.
Development and validation of High-Performance Liquid Chromatographic Technique to Estimate Pure Form of Loperamide Hydrochloride
Authors: V. Ramesh , Kunchanur Mahantesh , Patil Abhijeet , Kamble Praveen , Deshapande Raghunandan
Published: December 2022

A simple and reliable reverse phase high performance liquid chromatography RP-HPLC method was developed and validated to evaluate dissolution of Loperamide hydrochloride pharmaceutical dosage form. The method was developed on NovapakC18 150 mm x 4.6 mm 5 mum with acetonitrile 5545 vv as the mobile phase. The effluent was monitored by UV visible detector at 226 nm at 1.5 mlmin flow rate and 5micro injection volume. The calibration curve was linear against a concentration range of 0.2 ndash 4mugml. For system and method precision RSD values were found to be 0.16 and 1.03 respectively. Recovery was found to be in the range of 98 to 101. The RP-HPLC method developed has considerable importance and significant industrial applicability for QC quality control and analysis of loperamide hydrochloride tablet dosage form. This method can be applied in the routine analysis of pharmaceutical formulations of this drug.
Antioxidant and Antidiabetic Potential of Endophytic Fungal Fractions Recovered from Tinospora Cordifolia (Willd.) Leaves
Authors: Vasantrao Habbu Prasanna , Daniel Madagundi Smita , Hanumanth Kulkarni Venkatrao
Published: December 2022

BackgroundDiabetes mellitus DM is a severe metabolic disorder caused by lack of insulin secretion. Endophytes are microorganisms having the potentiality in colonizing internal tissues of the plant. Previous research reported that they are potential sources of novel secondary metabolites which are useful in medicine and agriculture.Objective In this study an attempt was made to isolate characterize and screen for endophytic fungi from the leaves of Tinospora cordifolia for in vitro antioxidant and antidiabetic activity.Materials and Methods Isolation of endophytic fungi was accomplished by established method. Pure culturable endophytic fungi were identified and characterized by PCR sequential analysis and phylogenetic studies. Chloroform ethyl acetate and n-butanol fractions of endophytic fungi were screened for in vitro free radical scavenging 2 2-diphenyl-1-picrylhydrazyl DPPH hydroxyl radical and reducing power assay and antidiabetic in alpha-amylase alpha- glucosidase and aldose reductase inhibitory assays.Results Two fungal endophytes were isolated TCLF-1 and TCLF-2 from leaves of Tinospora cordifolia with ethyl acetate T1EA T1EA and n-butanol T2nB T2nB. TCLF-1 and TCLF-2 fractions exhibited appreciable alpha-amylase alpha- glucosidase and aldose reductase inhibitory activity with significant IC50 values when compared with acarbose. Molecular characterization and phylogenetic studies identified TCLF-1 and TCLF-2 fungi as Trichoderma longibrachiatum isolate TL10 and Aspergillus versicolor isolate BAB-6580 respectively.Conclusion The results of the present study indicate that ethyl acetate and n-butanol fractions of TCLF-1 and TCLF-2 showed appreciable free radical scavenging and in vitro antidiabetic activity.
Assessment of Physical Activity Levels in Patients with Metabolic Syndrome
Authors: Sharon V Preethi , Prasanna Venkatesh , Sultana Anum , Meera NK , P Rasalkar Pavankumar
Published: December 2022

Background Metabolic Syndrome MetS is a cluster of conditions like abdominal obesity elevated blood glucose level elevated blood pressure and dyslipidemia which increases the risk of cardiovascular diseases CVDs. MetS is often associated with poorer health related quality of life.Objective The main objective of the study was to assess physical activity PA levels in MetS patients by administering International physical activity questionnaire IPAQ.MethodologyA six month prospective study was carried out in 156 patients of either gender in the age group 18-70 years attending a tertiary care hospital. The data was collected from patient case sheets and recorded in self designed data collection form. The PA was assessed by using IPAQ. Descriptive statistics and ANOVA were used to analyse data.Results The occurrence of MetS was higher in males 61.54 than in females 38.46. The maximum number of patients were in the age group 51-70 years and they were having low PA. It was observed that males had higher total scores in almost all the domains Work transportation domestic and leisure time when compared to females.Conclusion Physical activity assessment is important to educate the patients regarding the impact of lifestyle in control of complications arising due to MetS.
Development and Standardization of Chromatographic Technique for Quantification of Bicalutamide in Pure and Pharmaceutical Dosage Forms
Authors: V Ramesh , D Hiremath Ravindra , Kamble Praveen , Kunchanur Mahantesh , P Prerana
Published: December 2022

Background Bicalutamide BTM is a non-steroidal antiandrogen found to be available in various pharmaceutical dosage forms. Quantitative estimation of BTM is essential in the quality control laboratories of pharmaceutical industries.Objective The present research attempts to develop optimize and standardize chromatographic methodology for quantitative estimation of BTM in its pure and pharmaceutical dosage forms. Methodology The analysis was resolved by using a mobile phase acetonitrile methanol in the ratio of 6040 vv at a flow rate of 1 mlmin on an isocratic High Performance Liquid Chromatography HPLC system consisting of SPD-20 A20AV UV-VIS detector using C18 column at a wavelength of 275 nm. The developed method was subjected for standardization as per ICH guidelines. Results The retention time was found to be 4.7 minutes. The drug showed linear response between the concentration range from 10-100 mugmL. This method was found to be selective specific and precise with RSD Relative Standard Deviation less than 2.nbsp Conclusion The reported method was found to be simple precise and accurate for quantitative estimation of BTM in bulk and pharmaceutical dosage forms and can be employed for routine quality control analysis of BTM.
Preparation, Characterization and Screening of Silver Nanoparticles Using Phenolic Rich Fractions of Amaranthus Gangeticus L. for its in-vitro Anti-Oxidant, Anti-Diabetic and Anti-Cancer Activities.
Authors: Elias Akila , V Habbu Prasanna , Iliger Sudhir
Published: December 2022

Background Nowadays green nanotechnology-based approaches using plants have been acknowledged as harmless to the ecosystem and savvy approach with different biomedical applications.Aim In the current study we prepared silver nanoparticles AgNPs in combination with phenolic rich fractions of ethanolic extract of Amaranthus gangeticus L. Leaves using a suitable eco-friendly green-synthesis way to assess its in-vitro anti-oxidative anti-diabetic and anti-cancer potential against HeLa cells.Methods Plant mediated AgNPs were synthesized using phenolic rich fractions of ethanolic extract of Amaranthus gangeticus L. and characterization was done by UVndashvisible spectroscopy X-ray diffraction XRD Fourier Transform infrared FT-IR spectroscopy Scanning Electronic Microscopy SEM and TEM analysis. With the developing use of AgNPs in biomedical viewpoints the synthesized AgNPs were assessed for its pharmacological studies.Results The synthesized AgNPs have been confirmed by change of color which was identified by UVndashvisible spectroscopy at 450 nm due to surface plasmon resonance. The crystalline property of AgNPs was identified by its XRD pattern. Using FTIR it was confirmed that the phenolic functional group present in plant extract was responsible for the reduction of silver ion and the stabilization of AgNPs. The morphology of AgNPs were studied by SEM and TEM analysis. The particles were monodispersed white and spherical ranging from 10200 nm. With the developing use of AgNPs in biomedical viewpoint the synthesized AgNPs were assessed for their in-vitro anti-oxidative anti-diabetic and anti-cancer potential against HeLa cells. The results showed that the synthesized AgNPs exhibited good anti-diabetic anti-cancer activity. It was also observed to exhibit potent anti-oxidant activity. Conclusions From the present study the synthesized AgNPs using Amaranthus gangeticus L.nbsp were found to be very potent to treat the major diseases like cancer and diabetes.
Isolated Islets and Their Role in Diabetes Research
Authors: Samaddar Suman
Published: December 2022

Insulin-dependent diabetes is a multifactorial disorder characterized by beta-cell dysfunction that results in metabolic failure. Isolated islets remain a lucrative area of diabetes research as these are miniature organ systems that are able to secrete insulin upon appropriate stimulation without nervous control. This review encompasses the mechanisms responsible for beta cell destruction and regeneration from both beta and non-beta cells. Isolated islets are potential model for in vitro antidiabetic drug development screening of antihyperglycemic agents and elucidating their mechanism of actions. In this review various protocols of islet isolation have been discussed with their relative rates of success with regard to yield purity islet health morphology and stability in culture conditions. Various islet protecting compounds of natural origin along with their mechanism of action have also been reviewed.
Preparation of Additol Antiseptic and Disinfectant Solution
Authors: Richards M Paul , Anjum Nashra , Sharlet Ms. , Spoorthy Ms , Kanni Paranjothy , Shah Guddu , Viswanath B A
Published: December 2022

Background Antiseptics and disinfectants are mandatory equipment to maintain hygienic environment in hospitals and other health care settings which includes sanitizers preservatives and sporisites. Additol is a preparation in which the main chemical constituents are Terpineol castor oil basil oil propylene glycol and small quantity of liquid caramel made to sufficient quantity of one litre.Objective Castor oil and Sodium hydroxide gives the soap solution and followed by different analytical tests like saponification tests and all the values are validated and these are considered under the jurisdiction of FDA testing guidelines done for the activity and application of disinfectants. Currently during this pandemic it is used in medical settings to stop the spread of viruses.Methodology Castor oil and Sodium hydroxide NaOH were used in the preparation of soap solution. Terpineol oil basil oil propylene glycol and liquid caramel were further used in the preparation.Results The results were in good agreement and were performed under the testing guidelines. Tests were carried out to determine the antimicrobial activity and the obtained results were enclosed by standard limits. By following the standard procedure under the FDA testing guidelines prepared product Additol was compared with marketed product Dettol using Riedel-Walker Co-efficient test and the procured values were within the range.Conclusion The proposed Additol 1000 ml was evaluated for the disinfectant activity following standard procedure. The proposed disinfectant exhibited good disinfectant activity against the test microorganisms.
Why is publishing research important?
Authors: Raju Koneri Dr.
Published: December 2022

None
Preparation and Characterization of IPN Microspheres Containing Miglitol byUsing in House Synthesised Acrylamide Grafted Locust Bean Gum
Authors: Kumar P Ashok , K Manjunath , C Pawan , Kubbra Ayesha
Published: December 2022

Objective To increase the physicochemical stability swelling and drug release of the respective materials in biological conditions of natural and synthetic polymers hybridization is applied.Methodology In this study for the spatial and temporal controlled delivery of Miglitol a stable biodegradable and biocompatible interpenetrating polymer network IPN of acrylamide grafted locust bean gum Am-g-LBG and poly vinyl alcohol PVA was developed by emulsion crosslinking method. The IPN microspheres were prepared with the help of glutaraldehyde as a crosslinker for controlled delivery of Miglitol. Different LBG PVA ratios glutaraldehyde amounts and drug loading were used to optimise the formulation parameters. The drug entrapment efficiency swelling Fourier transform infrared spectroscopy FTIR X-ray diffraction XRD Scanning electron microscopy SEM and in vitro drug release profile of the microspheres were all investigated. Controlled release characteristics and drug release followed diffusion-controlled release pattern.Results Prolonged time drug release was observed without collapse of particle matrix. The percentage yield of IPN microspheres ranges from 95.56 F2 to 98.51. The drug entrapment efficiency percentage ranged from 70.12 plusmn 1.13 to 90.12 plusmn 0.95. After 12 hours drug dissolution the drug release percentage ranged from 61.45 to 92.87.Conclusion Thus IPN microspheres-based for controlled delivery of high water-soluble medications like miglitol may be a better option.
Preliminary Phytochemical Screening and Antibacterial Activity of Leaves ofPassiflora foetida Linn.
Authors: E Akila , Haroon Mohammed
Published: December 2022

Background Passiflora foetida is widely used in Indian traditional system of medicines to cure various human diseases.Aim The present study envisaged to select an appropriate local plant with anti-bacterial ability based on its folklore claim and to scientifically establish its anti-bacterial activity.Methods Shade dried powdered material of leaves of Passiflora foetida Linn. was used for the determination of physiochemical constants in accordance with WHO guidelines. Subsequent solvent extracts were prepared using petroleum ether chloroform ethanol and water. Each extract was further proceeded for detailed phytochemical study and anti-bacterial screening.Results The preliminary phytochemical screening revealed the chemical constituents of alkaloids flavonoids and phenolic compounds. In vitro antibacterial studies on the leaf extracts were carried out on medically important micro-organisms such as Escherichia coli Staphylococcus aureus Klebsiella pneumoniae Staphylococcus epidermis and Pseudomonas aeruginosa. Growth of all cultures was seen in the solvent control and in positive control. Petroleum ether and chloroform extracts showed inhibition of growth of microorganisms like Escherichia coli Staphylococcus aureus Klebsiella pneumoniae and Staphylococcus epidermis at 200 microgmL. No growth was seen for Pseudomonas aeruginosa at the concentration of 300 microgmL. Ethanol and aqueous extracts exhibited inhibitory effect on the growth of Escherichia coli Staphylococcus aureus Klebsiella pneumoniae and Staphylococcus epidermis at 200 microgmL and Pseudomonas aeruginosa at the concentration of 100 microgmL. A dose dependent inhibitory activity was observed.Conclusion From the above findings it was clear that the secondary metabolites present in leaf extracts of Passiflora foetida Linn are the source of plant-derived antimicrobial substances.
Mucoprotective and Mast Cell Stabilization Activities of Chromium (D-Phenylalanine)3 in Indomethacin-Induced Chronic Enterocolitis in Rats: In vitro Anti-Cancer Activity Using Caco-2 Cell Line
Authors: VP Veerapur , DS Suhas , TA Puneeth , MS Adithya , N Vinutha , E Manjunatha , S Vijayakumar
Published: December 2022

Objective The current research work was intended to establish the positive effects of chromium complex CrD-phe3 in indomethacin induced chronic enterocolitis. Additionally Caco-2 was used to elucidate the anti-cancer property of the title compound.Methodology Oral administration of indomethacin 1.5 mgkg twice quotidian for fourteen days was done to induce chronic enterocolitis in rats. Pre-treatment with chromium complex 30 amp 60 mugkg p.o for three days before induction was done and this continued up to the end of the study. Macroscopic scoring of the stomach jejunum and ileum was done to analyze the extent of damage and inflammation. Mesenteric mast cell degranulation counts serum and ileum tissue biochemical estimations and histopathological analysis were carried out. MTT assay was performed to identify the cytotoxicity of Cr D-Phe3 using Caco-2 cells.Results Oral administration of CrD-phe3 displayed a substantial reduction in the elevated macroscopical scores oxidative-nitrative stress biochemical parameters and mast cell degranulation induced by chronic treatment of indomethacin. The protective effect of the chromium complex was also established through histological inspection of the ileum. Further treatment of the title complex resulted in Caco-2 cell viability reduction and exhibited IC50 722.40 mM.Conclusion The pragmatic usefulness of the chromium complex might be owed to its antioxidative anti-inflammation anti-allergic and mucoprotective properties. In addition the title complex exhibited anticolorectal cancer.nbsp
Development and Validation of UV Spectrophotometric Method for Determination of Tacrolimus in Bulk and Capsule Dosage Form
Authors: R Suma , Karwa Preeti , Kusum Devi V
Published: December 2022

Background The present study aimed to develop and validate a rapid robust effective specific and accurate UV-Vis method for the determination of Tacrolimus in pharmaceutical dosage forms according to International Conference on Harmonization ICH Q2 R1 guidelines.Methodology The method development was done using phosphate buffer of pH 6.8. The method validation parameters like linearity precision accuracy robustness and ruggedness were assessed for the developed method. Pure drug solutions were prepared using Phosphate buffer pH 6.8 the aliquots of concentration range 1-10 microgmL and therefore the linear regression coefficient R2 was 0.999.Results The optimum lambdamax was at 295 nm. The developed method was precise within the interday and intraday studies and showed percentage RSD of 1.731 and 1.243 respectively. Thus a precise simple and cost-effective UV-Vis method for the determination of Tacrolimus was developed.Conclusion The method developed was found to be precise robust simple and cost-effective for the determination of Tacrolimus in bulk and in pharmaceutical dosage forms with regression coefficient R2 of 0.999.
Development and Evaluation of Elementary Osmotic Pump Tablet of Tizanidine Hydrochloride
Authors: K Halakatti Prabhu , Desai Anita , J Gaddimath Sachin , Mulabeli Naveenkumar , Patted Mahantesh , Danti Shrinivas , SG Rajanna
Published: December 2022

Aim The objective of present research work was to develop a sustained release osmotic tablet of Tizanidine HCl using suitable osmogens.Methodology Tizanidine HCl containing elementary osmotic tablets were prepared using a wet granulation process. Initially dried granules were screened for various pre compression evaluation parameters. Further dried granules were punched using 8 mm die cavity in a tablet punching machine. Thus developed tablets were evaluated for post compression parameters like tablet hardness thickness weight variation friability dissolution studies stability studies and scanning electron microscopy. Prior to dissolution studies an orifice was drilled on surface of tablet and the size of orifice was determined using SEM analysis.Results The pre compression evaluation for all the formulations had shown good results which in turn confirm excellent flow properties of granules. All formulations exhibited a weight gain of roughly 5-7. Dissolution studies confirm that the drug release pattern was controlled up to 24 hours formulation F9 had the maximum drug release around 97.27 at the end of 24 hours. The drug release was impacted by the amount of added osmogen microcrystalline cellulose and lactose concentrations. SEM studies were carried out to find the membrane surface diameter of the orifice and it was found to be 0.3 mm and revealed that the coat was intact devoid of pores after the dissolution. Kinetic study confirmed that all the formulations followed Zero order kinetics. Optimized formulation F9 was stable at room temperature and elevated temperature upto 30 days.Conclusion Developed Tizanidine HCl elementary osmotic pump tablets showed prolonged release of drug and found reproducible results for all the in vitro evaluation parameters.
Horizontal transfer of therfbcluster inLeptospirais a genetic determinant of serovar identity
Authors: Nieves Cecilia , Vincent Antony T , Zarantonelli Leticia , Picardeau Mathieu , Veyrier Frédéric J , Buschiazzo Alejandro
Published: December 2022

Leptospirabacteria comprise numerous species, several of which cause serious disease to a broad range of hosts including humans. These spirochetes exhibit large intraspecific variation, resulting in complex tabulations of serogroups/serovars that crisscross the species classification. Serovar identity, linked to biological/clinical phenotypes, depends on the structure of surface-exposed LPS. Many LPS biosynthesis–encoding genes reside within the chromosomicrfbgene cluster. However, the genetic basis of intraspecies variability is not fully understood, constraining diagnostics/typing methods to cumbersome serologic procedures. We now show that the gene content of therfbcluster strongly correlates withLeptospiraserovar designation. Whole-genome sequencing of pathogenicL. noguchii, including strains of different serogroups, reveals that therfbcluster undergoes extensive horizontal gene transfer. Therfbclusters from severalLeptospiraspecies disclose a univocal correspondence between gene composition and serovar identity. This work paves the way to genetic typing ofLeptospiraserovars, and to pinpointing specific genes within the distinctrfbclusters, encoding host-specific virulence traits. Further research shall unveil the molecular mechanism ofrfbtransfer amongLeptospirastrains and species.
Funcionamiento familiar y consumo de alcohol en adolescentes de la ciudad de Macas Ecuador
Authors: Escobar Armijos Lisbeth Carolina , Pilco Guadalupe Gina Alexandra
Published: December 2022

A escala planetaria el consumo de alcohol representa un problema para la salud pública, en la adolescencia múltiples factores contribuyen a la práctica de dicha conducta. La familia constituye el núcleo fundamental de la sociedad y su dinámica está estrechamente ligada al rol que desempeña cada integrante, al proporcionar componentes positivos de interacción, convivencia, desarrollo y conductas aceptables establecidas en un ambiente aparentemente estable. La investigación tuvo como objetivo evaluar la correlación entre funcionalidad familiar y niveles de consumo de alcohol en un grupo de adolescentes secundarios de edades comprendidas entre los 14 y 18 años. Se realizó un estudio cuantitativo, no experimental, con un alcance descriptivo y correlacional de corte trasversal, mediante la evaluación del cuestionario de Funcionalidad Familiar (FF-SIL) creado por De la Cuesta, Pérez, Louro y Bayarre (1996) y el cuestionario de Identificación de los Trastornos debidos al Consumo de Alcohol (AUDIT) elaborado por Saunders, Aasland, Babor, De La Fuente y Grant (1992). El análisis de resultados se realizó con el paquete estadístico SPSS (versión 25.0). Los resultados obtenidos dieron cuenta de una correlación estadísticamente negativa de la funcionalidad familiar con el consumo de alcohol (rₛ= -0.020, p= 0.705). Se concluye que un porcentaje mínimo de estudiantes presenta riesgo de consumo de alcohol, de la misma forma predomina la funcionalidad familiar, lo que constituye un factor protector y potencia la capacidad para evitar y disminuir la vulnerabilidad a esta conducta de consumo.
Factores de la imagen institucional, una perspectiva desde el Instituto Superior Tecnológico Cotacachi
Authors: Yacelga Anaguano Paulina
Published: December 2022

La presente investigación desarrolla un análisis documental de la imagen como parte de la planificación estratégica de las instituciones de educación superior. Este proceso genera criterios de individuación, donde la comunicación actúa como medio asociado. En este sentido, es la realidad propia de las instituciones, lo que permite comunicarse con magnitudes heterogéneas que están por encima y por debajo de ellas. Así, el objetivo del presente estudio, fue el explicar: cómo la imagen, representa el producto de la comunicación que emprende una organización con sus públicos internos y externos, siendo un bien intangible que tiene el potencial de llevar al entendimiento y compromiso con los grupos claves. El presente estudio contribuye a la comprensión de los fenómenos descritos desde dos entradas: en primer lugar, se enfoca en la determinación de la imagen desde su gestión, considerando su relación con las instituciones de Educación Superior, lo que corresponde a la recopilación y análisis de la información desde los espacios conceptuales. Finalmente, el estudio introduce la definición de los atributos, que explica, su proceso de determinación. Para llevar a cabo esta investigación se aplicó el método cualitativo a través de la revisión de literatura especializada, cuyos datos fueron extraídos de bases de datos académicas como: Scopus, Scielo, Dialnet, Redalyc, entre otras, y posteriormente, se realizó un análisis comparativo de la información obtenida en los documentos versus la revisión documental del Instituto Superior Tecnológico Cotacachi. Esto, con la finalidad de establecer la relación entre la teoría y su aplicación. Los resultados exponen que las dinámicas encontradas en la literatura guardan relación directa con aquellas establecidas en la institución, evidenciando su relevancia en el contexto de la educación superior.
Volatility Prediction via Hybrid LSTM Models with GARCH Type Parameters
Authors: Liu Mingyu , Ye Jing , Yu Lijie
Published: December 2022

Since the establishment of financial models for risk prediction, the measurement of volatility at risky market has improved, and its significance has also grown. For high-frequency financial data, the degree of investment risk, which has always been the focus of attention, is measured by the variance of residual sequence obtained following model regression. By integrating the long short-term memory (LSTM) model with multiple generalized autoregressive conditional heteroscedasticity (GARCH) models, a new hybrid LSTM model is used to predict stock price volatility. In this paper, three GARCH models are used, and the model that can best fit the data is determined.
EDUCAÇÃO SENSÍVEL E DE DIREITO: EXPERIÊNCIAS FORMATIVAS DOCENTES NO CAMPO DA EJA
Authors: ALMEIDA VERÔNICA , Barros Daltro de Castro Costa Andrea
Published: December 2022

Este texto resulta de uma pesquisa-formação que intencionou compreender experiências formativas de licenciandos/as, vivenciadas na Educação de Jovens e Adultos, a partir do Programa Residência Pedagógica, núcleo Pedagogia, da Universidade Federal da Bahia. Com discussão situada no entendimento da EJA como direito e na compreensão da formação docente como complexa e multirreferencial, tensiona concepções limitadas ao saber e ao saber-fazer e propõe ampliação dos itinerários formativos para contemplar, também, o saber-ser. Discorre sobre a construção de uma epistemologia docente fundada nas relações, apresentando eixos significantes engendrados no âmbito do Programa e que balizaram o trabalho pedagógico realizado. Apresenta experiências formativas destacadas pelos/as licenciandos/as como potencializadoras de uma educação sensível e apresenta como resultados o entendimento de que a apropriação simultânea de teorias curriculares, a compreensão da complexidade do fenômeno educativo e a experienciação de dinâmicas que abarcam a indissociabilidade entre o sensível e o inteligível, potencializam uma formação docente relevante para uma prática pedagógica inclusiva e humanizada, essencial aos sujeitos da EJA
A PRODUÇÃO TEXTUAL NO 2º ANO DO ENSINO FUNDAMENTAL EM UMA ESCOLA PÚBLICA DE FORTALEZA EM CONTEXTO DE VULNERABILIDADE SOCIAL
Authors: Freitas da Silva Janaína , Ribeiro Vanda Mendes , Marcucci Fernanda
Published: December 2022

Este artigo tem como objetivo analisar o que se ensina em Língua Portuguesa, em produção escrita, para alunos do 2o ano do Ensino Fundamental em uma escola situada em um contexto de vulnerabilidade, em Fortaleza. Este estudo compõe uma pesquisa que estuda a implementação do Programa de Aprendizagem na Idade Certa (PAIC). Por meio da perspectiva qualitativa de pesquisa, foram analisados um caderno do aluno e um livro didático, visando identificar a frequência com que aparecem atividades de produção escrita. Os artefatos analisados foram coletados por pesquisadores sêniores na escola-campo em setembro de 2019. O livro didático analisado é distribuído pelo Paic. Conclui-se que, no caderno analisado, há um maior número de atividades de produção escrita (13%) do que no livro didático (6%). A frequência com que atividades de produção escrita são solicitadas os alunos denota que, neste momento do processo de alfabetização, a preocupação da escola é maior com a aquisição do sistema de escrita e com a leitura do que com a produção de textos escritos contextualizados.
AS INFLUÊNCIAS INTERNACIONAIS NO ENSINO SUPERIOR PÚBLICO NO BRASIL: UM ESTUDO SOBRE A UFPR
Authors: Pianovski Vieira Alboni Marisa Dudeque , Da Silva Gabriel Fernandes , De Lima Seabra Michelle Ariane
Published: December 2022

Este estudo analisa a influência dos organismos internacionais no ensino superior, buscando desvelar como os Planos de Desenvolvimento Institucional (PDI), com maior ou menor ênfase, refletem suas orientações. Desse modo, tomando como fonte os documentos emitidos pelo Banco Mundial (BM), examinou-se de que forma tais recomendações influenciam o ensino superior público brasileiro. Trata-se de estudo de caso bibliográfico e documental, apoiado no PDI da Universidade Federal do Paraná (UFPR) de 2019 a 2021. Como referencial teórico, adotaram-se os estudos de Veiga (1998), Mota (2019) e Lima e Gandin (2019). Como resultado, constatou-se que o Banco Mundial emitiu recomendações sobre o financiamento das instituições de ensino superior públicas e indicou diversas estratégias para implementar os conceitos de diferenciação institucional e diversificação nas políticas educacionais. No caso da UFPR, essas influências são perceptíveis quando se trata do “produto UFPR”, condição que coloca os processos formativos vinculados às oscilações do mercado.
O ENSINO REMOTO PARTICIPATIVO NA PÓS-GRADUAÇÃO: JOGOS COMO TEMÁTICA PARA DISCUTIR O ENSINO DE CIÊNCIAS
Authors: Pita-Carmo Marcelle , Barreto Jéssica , Vasconcellos Marcelo , Atella Geórgia
Published: December 2022

Desde o início do isolamento social motivado pela pandemia de COVID-19 em 2020, o ensino remoto tornou-se necessário para a continuidade das atividades educativas de vários níveis de ensino no Brasil, tendo as Tecnologias de Informação e Comunicação (TIC) como apoio para a interação e ensino-aprendizagem. Nesse contexto, o presente estudo busca discutir como a criação de uma disciplina remota de jogos para o ensino de ciências permite desenvolver discussões sobre ciência e questões sociais de maneira participativa na pós-graduação. Para isso, são apresentados diferentes fatores necessários ao melhor aproveitamento das disciplinas em ambientes digitais, além de critérios utilizados para a organização prévia das ferramentas. Como resultado, são apresentados os materiais e ferramentas formulados, além de feedbacks dos cursistas da disciplina ministrada entre agosto e outubro de 2020. Dessa forma, o estudo permite apontar estratégias não apenas para o ensino remoto, mas também a importância dos jogos como fenômeno tecnológico e cultural, e que permitem discutir diferentes questões sociais.
Features of the development of a simulation model of the gearbox assembly of the spacecraft drive
Authors: Gorbunov A. V. , Zheltyshev O. I. , Sablin A. S. , Zagainov M. A. , Kuznetsov S. A.
Published: December 2022

The gear assembly of the rotational motion drive of the spacecraft system is considered. Such gear assemblies are one of the main elements of spacecraft systems with the use of large-sized structures that are folded in the transport position. Due to the development of computer technology, it becomes possible to create more accurate simulation models of such structures. The aim of the work is to create a simulation model that allows to analyze emerging loads on a wave transmission, which is part of the gear assembly of the spacecraft drive. Section one discusses the process of creating a finite element model of the drive. One of the features of creating a finite element model is a method of taking into account the preliminary stress-strain state in the structural elements of the wave transmission. Section two analyzes the data obtained during the calculation. The obtained equivalent stresses on the structural parts, contact stresses in the gear engagement are investigated, the torsional rigidity of the gearbox elements is determined, various tooth positions are considered during movement. The influence of loads applied to the output link of the gearbox on the surface of the gears is investigated. The obtained simulation model allows us to consider similar gear assemblies in which wave transmissions are used.
Preparation of the surface of parts from titanium alloys for gluing with parts from polymer composite materials
Authors: Udod L. S. , Nagovitsin V. N.
Published: December 2022

A method has been developed for preparing the surface of titanium alloys, which provides an increase in the strength characteristics of titanium–composite adhesive joints. When preparing the surface of parts made of titanium alloys, it is not enough to use only mechanical treatment, and when combined with chemical surface treatment, the formation of strong bonds is possible. Surface preparation is carried out by forming a relief structure by applying corrugations on the machine, followed by removal of the oxide film and the formation of a high degree of microroughness by etching in a mixture of acids. Preparing the surface of titanium surfaces with a new method allows minimizing the influence of the human factor, improving the adhesion characteristics of the surfaces of parts, and increasing the time interval between mechanical preparation and gluing. Studies have been carried out to determine the ultimate strength in shear, bending, torsion, compression, tension, as well as to determine the effect of the gap in time intervals between mechanical surface preparation and gluing on the strength characteristics of the adhesive joint on titanium–composite samples with different titanium surface preparation. The analysis of the influence of methods of surface treatment of parts made of titanium alloys on the strength of the adhesive joint is carried out, taking into account the influence of thermal exposure in vacuum conditions, simulating the operation of a spacecraft in space conditions.
AKTUALISASI KARAKTER KEWIRAUSAHAAN KEPALA PAUD MENUJU KETAHANAN LEMBAGA PAUD PASCA PANDEMIC COVID 19
Authors: Wiyani Novan Ardy
Published: December 2022

This paper examines the actualization of the entrepreneurship character of the PAUD headmaster in order to maintain the existence of PAUD institutions in the post covid 19 pandemic, where it is known that for more than two years covid 19 has threatened the existence of PAUD institutions. The purpose of this article is to provide a description to the headmaster of PAUD both in the Playgroup, Kindergarten and Raudhatul Athfal about the various efforts that can be made to maintain the existence of PAUD institutions during the post covid 19 pandemic through actualization of the entrepreneurship character of the head of PAUD. This research is a type of library research, where data is collected using documentation techniques. The data that has been collected is then analyzed using content analysis techniques. The results showed that there are six entrepreneurial characters that can be actualized by the PAUD headmaster, including self-confidence, productivity-oriented, leadership, originality, future-oriented and honest and diligent. With the actualization of the six entrepreneurship characters of the PAUD headmaster above, it is hoped that the existence of PAUD institutions in the post-covid-19 pandemic can be maintained.