bluuebunny/crossref_metadata_2025 huggingface dataset:
Emetine inhibits Zika and Ebola virus infections through two molecular mechanisms: inhibiting viral replication and decreasing viral entry
Authors: Yang Shu , Xu Miao , Lee Emily M , Gorshkov Kirill , Shiryaev Sergey A. , He Shihua , Sun Wei , Cheng Yu-Shan , Hu Xin , Tharappel Anil Mathew , Lu Billy , Pinto Antonella , Farhy Chen , Huang Chun-Teng , Zhang Zirui , Zhu Wenjun , Wu Yuying , Zhou Yi , Song Guang , Zhu Heng , Shamim Khalida , Martínez-Romero Carles , García-Sastre Adolfo , Preston Richard A. , Jayaweera Dushyantha T. , Huang Ruili , Huang Wenwei , Xia Menghang , Simeonov Anton , Ming Guoli , Qiu Xiangguo , Terskikh Alexey V. , Tang Hengli , Song Hongjun , Zheng Wei
Published: June 2018

The re-emergence of Zika virus (ZIKV) and Ebola virus (EBOV) poses serious and continued threats to the global public health. Effective therapeutics for these maladies is an unmet need. Here, we show that emetine, an anti-protozoal agent, potently inhibits ZIKV and EBOV infection with a low nanomolar half maximal inhibitory concentration (IC50) in vitro and potent activity in vivo. Two mechanisms of action for emetine are identified: the inhibition of ZIKV NS5 polymerase activity and disruption of lysosomal function. Emetine also inhibits EBOV entry. Cephaeline, a desmethyl analog of emetine, which may be better tolerated in patients than emetine, exhibits a similar efficacy against both ZIKV and EBOV infections. Hence, emetine and cephaeline offer pharmaceutical therapies against both ZIKV and EBOV infection.
Publisher Correction: Direct reprogramming of fibroblasts into skeletal muscle progenitor cells by transcription factors enriched in undifferentiated subpopulation of satellite cells
Authors: Ito Naoki , Kii Isao , Shimizu Noriaki , Tanaka Hirotoshi , Takeda Shin’ichi
Published: June 2018

A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper.
Investigating the Glycating Effects of Glucose, Glyoxal and Methylglyoxal on Human Sperm
Authors: Nevin Clare , McNeil Lauren , Ahmed Nessar , Murgatroyd Chris , Brison Daniel , Carroll Michael
Published: June 2018

Glycation is the non-enzymatic reaction between reducing sugars, such as glucose, and proteins, lipids or nucleic acids, producing Advanced Glycation End (AGE) products. AGEs, produced during natural senescence as well as through lifestyle factors such as diet and smoking, are key pathogenic compounds in the initiation and progression of diabetes. Importantly, many of these factors and conditions also have influence on male fertility, affecting sperm count and semen quality, contributing to the decreasing trend in male fertility. This study investigated the impact of AGEs on sperm damage. In vitro sperm glycation assays were used to determine the levels and localization of the potent AGE compound, carboxymethyl-lysine (CML) in response to treatment with the glycating compounds glucose, glyoxal and methylglyoxal. Sperm function assays were then used to assess the effects of glycation on motility and hyaluronan binding, and levels of oxidative DNA damage were analyzed through measurement of the marker, 8-oxoguanine. Results showed that glyoxal, but not glucose or methylglyoxal, induced significant increases in CML levels on sperm and this correlated with an increase in 8-oxoguanine. Immunocytochemistry revealed that AGEs were located on all parts of the sperm cell and most prominently on the head region. Sperm motility and hyaluronidase activity were not adversely affected by glycation. Together, the observed detrimental effects of the increased levels of AGE on DNA integrity, without an effect on motility and hyaluronidase activity, suggest that sperm may retain some fertilizing capacity under these adverse conditions.
Exploring the Relationship between Blood Flux Signals and HRV following Different Thermal Stimulations using Complexity Analysis
Authors: Wang Guangjun , Jia Shuyong , Li Hongyan , Wang Ze , Zhang Weibo
Published: June 2018

To investigate the relationship between local blood flux and heart rate variability following different thermal stimulations, healthy subjects were recruited and subject to different thermal stimulations on the right forearm. Multiscale entropy and multiscale fuzzy entropy were used to measure the complexity of the local blood flux, and the approximate entropy was calculated to evaluate the HRV complexity. The results indicated that thermal stimulation significantly increased local blood flux and that different temperature stimulations resulted in different complexities in local blood flux. A 42 °C or 44 °C thermal stimulation, other than stimulations below 42 °C, resulted in a moderate correlation between local blood flux and heart rate variability complexity. The results provide a new perspective in terms of complexity to explore the relationship between skin blood flux signals and cardiac function.
Developmental and reproductive response of Brachmia macroscopa (Lepidoptera: Gelechiidae) to three host plants
Authors: Ma Li , Li Ni , Wang Xing , Liu Yan , Su Ming-Zhu , Huang Guo-Hua
Published: June 2018

The sweet potato leaf folder, Brachmia macroscopa Meyrick (Lepidoptera: Gelechiidae), which is a significant pest of plants in the family Convolvulaceae, is rapidly expanding its range in South China and other subtropical regions. Studies were designed to examine the effects of three different host plants (sweet potato, Ipomoea batatas (L.) Lam.; water spinach, I. aquatica Forsskål; and morning glory, Pharbitis purpurea (L.)) on the development and life table parameters of B. macroscopa under laboratory conditions. We found that the intrinsic rates of increase of B. macroscopa were 0.17 ± 0.004, 0.21 ± 0.005 and 0.16 ± 0.004 on I. batatas, I. aquatica and P. purpurea, respectively. The highest net reproduction rate was 158.06 ± 18.22 per female reared on I. aquatica. The larvae had five instars when reared on I. batatas and I. aquatica, but required six instars on P. purpurea. The mean generation lengths of B. macroscopa ranged from 24.32 ± 0.18 days when reared on I. aquatica to 29.40 ± 0.24 days on P. purpurea. The survival of all stage and fecundity curves was intuitively manipulated using the age-stage-structured and two-sex population life table method, to enable comprehensive descriptions of the stage and population trends of B. macroscopa on the three Convolvulaceae plants. Our results indicated that I. batatas and I. aquatica were more suitable host plants than P. purpurea.
GATA-4-expressing mouse bone marrow mesenchymal stem cells improve cardiac function after myocardial infarction via secreted exosomes
Authors: He Ji-Gang , Li Hong-Rong , Han Jin-Xiu , Li Bei-Bei , Yan Dan , Li Hong-Yuan , Wang Ping , Luo Ying
Published: June 2018

This study aimed to investigate whether exosomes secreted by mouse GATA-4-expressing bone marrow mesenchymal stem cells (BMSCs) could induce BMSC differentiation into myocyte precursors, decrease cardiomyocyte apoptosis, and improve cardiac function following myocardial infarction (MI). BMSCs were transduced with a lentivirus carrying a doxycycline (DOX)-inducible GATA-4 or control lentivirus, and secreted exosomes from these BMSCs were collected and co-cultured with BMSCs or cardiomyocytes under hypoxic and serum free conditions. Furthermore, exosomes were injected into mice 48 h after MI. Cardiac function was evaluated by echocardiography at 48, 72, and 96 h after exosome treatment. Quantitative PCR showed that co-culture of BMSCs with GATA-4-BMSC exosomes increased cardiomyocyte-related marker expression. Co-culture of GATA-4-BMSC exosomes with cardiomyocytes in anoxic conditions decreased apoptosis as detected by flow cytometry. Injection of GATA-4-BMSC exosomes in mice 48 h after MI increased cardiac function over the next 96 h; increased cardiac blood vessel density and number of c-kit-positive cells and decreased apoptotic cardiomyocyte cells were also observed. Differential expression of candidate differentiation- and apoptosis-related miRNAs and proteins that may mediate these effects was also identified. Exosomes isolated from GATA-4-expressing BMSCs induce differentiation of BMSCs into cardiomyocyte-like cells, decrease anoxia-induced cardiomyocyte apoptosis, and improve myocardial function after infarction.
A simple colorimetric analytical assay using gold nanoparticles for specific detection of tetracycline in environmental water samples
Authors: Qi Mengyu , Tu Chunyan , Dai Yuanyuan , Wang Weiping , Wang Aijun , Chen Jianrong
Published: June 2018

In this work, an aptamer-based colorimetric method for the detection of tetracycline (TC) was established by employing gold nanoparticles (AuNPs) as the colorimetric probe.
Hydroxide-ion-conductive gas barrier films based on layered double hydroxide/polysulfone multilayers
Authors: Xu Xiaozhi , Wang Lumei , Wang Jiajie , Yin Qing , Dong Siyuan , Han Jingbin , Wei Min
Published: June 2018

Hybrid films were fabricated via the layer-by-layer assembly of layered double hydroxide (LDH) nanoplates and quaternary ammonium grafted polysulfone (QAPSF), and showed dual functionality with both gas barrier and hydroxide ion conductivity properties.
Plasmonic resonance enhanced photoelectrochemical aptasensors based on g-C3N4/Bi2MoO6
Authors: Qiu Zhenli , Shu Jian , Tang Dianping
Published: June 2018

An in-depth exploration associated with localized surface plasmon resonance between g-C3N4/Bi2MoO6 and gold nanoparticles has been conducted for highly efficient photoelectrochemical aptasensors under ultraviolet and visible light irradiation.
Measuring the orientation of a single CdSe/CdS nanocrystal at the end of a near-field tip for the realization of a versatile active SNOM probe
Authors: Jazi R. , Ung T. P. L. , Maso P. , Colas Des Francs G. , Nasilowski M. , Dubertret B. , Hermier J.-P. , Quélin X. , Buil S.
Published: June 2018

The orientation a CdSe/CdS nanocrystal attached at the end of a scanning near field optical microscope (SNOM) tip is analyzed by its coupling with a flat gold layer.
Binary mixtures of charged colloids: a potential route to synthesize disordered hyperuniform materials
Authors: Chen Duyu , Lomba Enrique , Torquato Salvatore
Published: June 2018

A new route to fabricate large samples of 2D disordered hyperuniform materials via self-assembly of mixtures of charged colloids.
Non-uniform lithium-ion migration on micrometre scale for garnet- and NASICON-type solid electrolytes studied by 7Li PGSE-NMR diffusion spectroscopy
Authors: Hayamizu Kikuko , Seki Shiro , Haishi Tomoyuki
Published: June 2018

The migration behaviours of Li+ in three garnet- and one NASICON-type solid oxide electrolytes were studied on the micrometre scale by pulsed-gradient spin-echo (PGSE) 7Li NMR diffusion spectroscopy to clarify common and specific characteristics of each electrolyte.
Correction: Solvation of Li+ by argon: how important are three-body forces?
Authors: Prudente Frederico V. , Marques Jorge M. C. , Pereira Francisco B.
Published: June 2018

Correction for ‘Solvation of Li+ by argon: how important are three-body forces?’ by Frederico V. Prudente et al., Phys. Chem. Chem. Phys., 2017, 19, 25707–25716.
Photovoltaic device performance of pure, manganese (Mn2+) doped and irradiated CuInSe2 thin films
Authors: Prabukanthan P. , Lakshmi R. , Harichandran G. , Tatarchuk Tetiana
Published: June 2018

A successful single step ECD of pure CuInSe2 and Mn2+ doped CuInSe2 thin films was carried out. 5 mole% Mn doped CuInSe2 thin film-based solar cells exhibit better PCE.
Xerogel modified diatomaceous earth microparticles for controlled drug release studies
Authors: Uthappa U. T. , Sriram G. , Brahmkhatri Varsha , Kigga Madhuprasad , Jung Ho-Young , Altalhi Tariq , Neelgund Gururaj M. , Kurkuri Mahaveer D.
Published: June 2018

An alternative facile approach for the surface modification of naturally available diatoms with xerogel for controlled drug release applications.
A comparative study of the effects of terminal aromatic moieties in spirobifluorene core-based diketopyrrolopyrrole non-fullerene acceptors
Authors: Chen Zhi-Cai , Xie Yuan , Yu Yuan-Yuan , Wu Hong-Bin , Wan Jun-Hua
Published: June 2018

Trifluorobenzene and fused-ring moieties (benzo[b]thiophene and benzo[b]furan) were separately introduced into promising DPP-based nonfullerenes SF-DPP-EH to extend conjugation.
Rhodamine–benzothiazole conjugate as an efficient multimodal sensor for Hg2+ ions and its application to imaging in living cells
Authors: Sakthivel Perumal , Sekar Karuppannan , Sivaraman Gandhi , Singaravadivel Subramanian
Published: June 2018

A rhodamine B dye bearing a benzothiazole conjugate is designed and synthesized, it shows a highly selective and sensitive naked-eye color change and turn-on fluorescence response to Hg2+ ions.
Ion exchange for synthesis of porous CuxO/SnO2/ZnSnO3 microboxes as a high-performance lithium-ion battery anode
Authors: Zhang Min , Ma Jingjing , Zhang Yidan , Lu Leidan , Chai Yaqin , Yuan Ruo , Yang Xia
Published: June 2018

Integrating other oxides and ZnSnO3 with porous structures can accommodate volume expansion and enhance the electrochemical performance.
The halogen bond: a new avenue in recognition and self-assembly
Authors: Resnati Giuseppe , Pennington William T.
Published: June 2018

Welcome to this themed issue of NJC entitled: ‘The halogen bond: a new avenue in recognition and self-assembly’.
An ultrafine platinum–cobalt alloy decorated cobalt nanowire array with superb activity toward alkaline hydrogen evolution
Authors: Wang Ziqiang , Ren Xiang , Luo Yonglan , Wang Liang , Cui Guanwei , Xie Fengyu , Wang Hongjing , Xie Ying , Sun Xuping
Published: June 2018

Ultrafine PtCo nanoparticles decorated Co nanowires on Ti mesh deliver an ultrahigh current density of 46.5 mA cm−2 at an overpotential of 70 mV in 1.0 M KOH.
86 GHz SiO maser survey of late-type stars in the Inner Galaxy
Authors: Messineo M. , Habing H.J. , Sjouwerman L. O. , Omont A. , Menten K. M.
Published: June 2018

We present an 86 GHz SiO (v = 1,  J = 2 → 1) maser search toward late-type stars located within |b|< 0.​​°5 and 20° <  l <  50°. This search is an extension at longer longitudes of a previously published work. We selected 135 stars from the MSX catalog using color and flux criteria and detected 92 (86 new detections). The detection rate is 68%, the same as in our previous study. The last few decades have seen the publication of several catalogs of point sources detected in infrared surveys (MSX, 2MASS, DENIS, ISOGAL, WISE, GLIMPSE, AKARI, and MIPSGAL). We searched each catalog for data on the 444 targets of our earlier survey and for the 135 in the survey reported here. We confirm that, as anticipated, most of our targets have colors typical of oxygen-rich asymptotic giant branch (AGB) stars. Only one target star may have already left the AGB. Ten stars have colors typical of carbon-rich stars, meaning a contamination of our sample with carbon stars ≲1.7%.
MOLPOP-CEP: an exact, fast code for multi-level systems
Authors: Asensio Ramos Andrés , Elitzur Moshe
Published: June 2018

We present MOLPOP-CEP, a universal line transfer code that allows the exact calculation of multi-level line emission from a slab with variable physical conditions for any arbitrary atom or molecule for which atomic data exist. The code includes error control to achieve any desired level of accuracy, providing full confidence in its results. Publicly available, MOLPOP-CEP employs our recently developed coupled escape probability (CEP) technique, whose performance exceeds other exact methods by orders of magnitude. The program also offers the option of an approximate solution with different variants of the familiar escape probability method. As an illustration of the MOLPOP-CEP capabilities we present an exact calculation of the Spectral Line Energy Distribution (SLED) of the CO molecule and compare it with escape probability results. We find that the popular large-velocity gradient (LVG) approximation is unreliable at large CO column densities. Providing a solution of the multi-level line transfer problem at any prescribed level of accuracy, MOLPOP-CEP is removing any doubts about the validity of its final results.
Interobserver Agreement for Contrast-Enhanced Ultrasound (CEUS)-Based Standardized Algorithms for the Diagnosis of Hepatocellular Carcinoma in High-Risk Patients
Authors: Schellhaas Barbara , Pfeifer Lukas , Kielisch Christian , Goertz Ruediger , Neurath Markus , Strobel Deike
Published: June 2018

Objectives This pilot study aimed at assessing interobserver agreement with two contrast-enhanced ultrasound (CEUS) algorithms for the diagnosis of hepatocellular carcinoma (HCC) in high-risk patients.

Methods Focal liver lesions in 55 high-risk patients were assessed independently by three blinded observers with two standardized CEUS algorithms: ESCULAP (Erlanger Synopsis of Contrast-Enhanced Ultrasound for Liver Lesion Assessment in Patients at risk) and ACR-CEUS-LI-RADSv.2016 (American College of Radiology CEUS-Liver Imaging Reporting and Data System). Lesions were categorized according to size and ultrasound contrast enhancement in the arterial, portal-venous and late phase. Interobserver agreement for assessment of enhancement pattern and categorization was compared between both CEUS algorithms. Additionally, diagnostic accuracy for the definitive diagnosis of HCC was compared. Histology and/or CE-MRI and follow-up served as reference standards.

Results 55 patients were included in the study (male/female, 44/ 11; mean age: 65.9 years). 90.9 % had cirrhosis. Histological findings were available in 39/55 lesions (70.9 %). Reference standard of the 55 lesions revealed 48 HCCs, 2 intrahepatic cholangiocellular carcinomas (ICCs), and 5 non-HCC-non-ICC lesions. Interobserver agreement was moderate to substantial for arterial phase hyperenhancement (ĸ = 0.53 – 0.67), and fair to moderate for contrast washout in the portal-venous or late phase (ĸ = 0.33 – 0.53). Concerning the CEUS-based algorithms, the interreader agreement was substantial for the ESCULAP category (ĸ = 0.64 – 0.68) and fair for the CEUS-LI-RADS® category (ĸ = 0.3 – 0.39). Disagreement between observers was mostly due to different perception of washout.

Conclusion Interobserver agreement is better for ESCULAP than for CEUS-LI-RADS®. This is mostly due to the fact that perception of contrast washout varies between different observers. However, interobserver agreement is good for arterial phase hyperenhancement, which is the key diagnostic feature for the diagnosis of HCC with CEUS in the cirrhotic liver.
Bullöses Pemphigoid unter Pembrolizumab-Therapie eines metastasierten malignen Melanoms
Authors: Troyanova-Slavkova S. , Schierle K. , Beloia S.-D. , Kowalzick L.
Published: June 2018

Pembrolizumab ist ein monoklonaler Antikörper, der an den PD-1-Rezeptor auf T-Zellen bindet und die Interaktion mit den Liganden PD-L1 und PD-L2 auf Krebszellen hemmt. Pembrolizumab wird zunehmend für die Behandlung von fortgeschrittenen Tumorerkrankungen, v. a. für metastasierte maligne Melanome, aber auch für nicht-kleinzelliges Lungenkarzinom, Urothelkarzinom und Nierenkarzinom angewendet. Trotz der beeindruckenden klinischen Wirkungen sind die Checkpoint-Inhibitoren mit einer Reihe von Nebenwirkungen verbunden, die als immunbezogene unerwünschte Ereignisse bekannt sind. Zu den bekannten Nebenwirkungen dieser Therapie gehören neben kutanen Nebenwirkungen wie Hautausschlag auch Müdigkeit, Schilddrüsendysfunktion, immunvermittelte Pneumonitis und Kolitis. Zu den kutanen unerwünschten Ereignissen gehören u. a. Pruritus, lichenoide Dermatitis, Psoriasis sowie blasenbildende Erkrankungen wie bullöses Pemphigoid. Die Nebenwirkungen werden durch die immunoregulatorischen Mechanismen des Medikaments erklärt. Hier berichten wir über den Fall eines 72-jährigen Patienten mit metastasiertem malignen Melanom und Prostatakarzinom als Zweittumor, der unter einer Therapie mit Pembrolizumab rasch sowohl eine stabile Erkrankung des Tumors hatte, aber auch zuerst eine immunvermittelte Pneumonitis und im Verlauf dann auch ein bullöses Pemphigoid entwickelte. Dieser Fall ergänzt die Liste möglicher kutaner Nebenwirkungen einer PD-1-Inhibitor-Therapie.
KIAA1199 Biomarker and Ultrasonographic Findings in Rheumatoid
Arthritis Patients and their Correlation with Disease Activity
Authors: Selim Zahraa Ibrahim , El-Hakeim Eman H , Omran Eman Ahmed Hamed , Idriss Naglaa K. , Gaber Marwa A. , Ross Sylvia V
Published: June 2018

Introduction Rheumatoid arthritis (RA) is an autoimmune disease that
affects multiple joints causing joint destruction. KIAA1199 is a novel
angiogenic biomarker derived from fibroblast-like synoviocytes (FLS) it has a
role in acceleration and proliferation of FLS and activation of angiogenic
signaling pathways leading to erosion of cartilage and bone. Musculoskeletal
ultrasound (MUSU) and Power Doppler (PDUS) directly visualizing the synovial
membrane vessels, which is important in providing very early information on the
changes in synovitis activity during the course of the inflammatory joint
disease

Objective To assess the serum level of angiogenic biomarker KIAA1199 in
RA patients and its correlation with MSUS, PDUS findings, and the disease
activity Patients and methods: Fifty RA patients and 40 healthy control persons
age and sex-matched were recruited in this study, KIAA1199 was assessed in the
serum of patients and controls, MSUS and PDUS were done for the wrist, elbow,
and knee joints for all RA patients

Results Serum KIAA1199 level was significantly higher among RA patients
4.36±1.22 ng/dl compared to control group
2.87±0.51 ng/dl (p<0.001). There was a highly
significant correlation between KIAA1199 level and DAS28 (p=0.004), and
there was a significant correlation between the PDUS with KIAA1199 level and
DAS28 (p=0.001, 0.002 respectively) in wrist joints

Conclusion KIAA1199 is a new pathway that enhancing cell proliferation
and angiogenesis. Serum KIAA1199 level may be a useful biomarker for RA
activity, and therapeutic target in RA. PDUS correlates significantly with
clinical findings and novel angiogenic biomarker in RA patients.
Interview mit Hauke Heissmeyer: „Sie müssen standardisieren – auch und gerade wegen der medizinischen Qualität“
Authors: Epping Bernhard
Published: June 2018

Hauke Heißmeyer, der kaufmännische Geschäftsführer der Orthopädischen Universitätsklinik Friedrichsheim in Frankfurt am Main erklärt, wie der Einkauf in einer orthopädischen Fachklinik mit den Schwerpunkten Endoprothetik und Wirbelsäulenchirurgie funktioniert.
Green Synthesis of Haloformates from Olefins Using Formic Acid as Reactant, Protonic Acid, and Solvent
Authors: Wang Ligeng , Hu Jun , Zhang Hualong , Yu Qin , Feng Chun
Published: June 2018

Bromoformates and iodoformates are successfully synthesized in high yields with regioselectivity and stereoselectivity by using ZnAl-BrO3
– layered double hydroxides (LDHs) and KX (X = Br, I) in the presence of formic acid (HCOOH). The protocol exploits the versatile function of formic acid as solvent, nucleophilic reagent, and acidic medium simultaneously, simplifying the reaction and separation of the products.
Semiautomated System for Nonurgent, Clinically Significant Pathology Results
Authors: O'Connor Stacy , Khorasani Ramin , Pochebit Stephen , Lacson Ronilda , Andriole Katherine , Dalal Anuj
Published: June 2018

Background Failure of timely test result follow-up has consequences including delayed diagnosis and treatment, added costs, and potential patient harm. Closed-loop communication is key to ensure clinically significant test results (CSTRs) are acknowledged and acted upon appropriately. A previous implementation of the Alert Notification of Critical Results (ANCR) system to facilitate closed-loop communication of imaging CSTRs yielded improved communication of critical radiology results and enhanced adherence to institutional CSTR policies.

Objective This article extends the ANCR application to pathology and evaluates its impact on closed-loop communication of new malignancies, a common and important type of pathology CSTR.

Materials and Methods This Institutional Review Board-approved study was performed at a 150-bed community, academically affiliated hospital. ANCR was adapted for pathology CSTRs. Natural language processing was used on 30,774 pathology reports 13 months pre- and 13 months postintervention, identifying 5,595 reports with malignancies. Electronic health records were reviewed for documented acknowledgment for a random sample of reports. Percent of reports with documented acknowledgment within 15 days assessed institutional policy adherence. Time to acknowledgment was compared pre- versus postintervention and postintervention with and without ANCR alerts. Pathologists were surveyed regarding ANCR use and satisfaction.

Results Acknowledgment within 15 days was documented for 98 of 107 (91.6%) pre- and 89 of 103 (86.4%) postintervention reports (p = 0.2294). Median time to acknowledgment was 7 days (interquartile range [IQR], 3, 11) preintervention and 6 days (IQR, 2, 10) postintervention (p = 0.5083). Postintervention, median time to acknowledgment was 2 days (IQR, 1, 6) for reports with ANCR alerts versus 6 days (IQR, 2.75, 9) for reports without alerts (p = 0.0351). ANCR alerts were sent on 15 of 103 (15%) postintervention reports. All pathologists reported that the ANCR system positively impacted their workflow; 75% (three-fourths) felt that the ANCR system improved efficiency of communicating CSTRs.

Conclusion ANCR expansion to facilitate closed-loop communication of pathology CSTRs was favorably perceived and associated with significant improved time to documented acknowledgment for new malignancies. The rate of adherence to institutional policy did not improve.
The Influence of Serotonin on Clot Retraction and the Thrombelastogram
Authors: Deutsch E , Martiny K
Published: June 2018

1. Normal platelets are necessary for induction of normal clot retraction.

2. Serotonin does not induce retraction in human platelet-free plasma-clots or enhance clot firmness as measured in the coagulogram.

3. Serotonin does not improve clot retraction or firmness in plasma clots with sub-optimal platelet counts.

4. Methylserotonin inhibits clot retraction of platelet-rich plasma to a certain extent in moderate doses, whereas, high doses are ineffective. BOL 148 has a similar, but less significant action. There is a possibility that these effects are specific antiserotonin-effects.

5. LSD 25 was ineffective in all concentrations used.

6. Largactil and reserpin inhibit retraction in high doses. There seems to be a non specific effect caused by the high dose.

7. Reserpine does not release a retraction-inducing agent from the platelets, which could be detected in the centrifuged platelet-free plasma used for the incubation.

8. Serotonin does not replace the retraction-cofactor of Hartert, or the dialyzable factor of Lüscher in synthetic clotting substrates.

9. Serotonin is of no essential value in inducing normal retraction of human plasma clots.
Perioperative Challenges in an Infant with Uncorrected “Pink-Tetralogy of Fallot”—for Excision of Choroid Plexus Papilloma: A Case Report
Authors: Pearl Julia , Lionel Karen Ruby , Mariappan Ramamani
Published: June 2018

Perioperative management of an infant with intracranial tumor and congenital cyanotic heart disease poses a lot of challenges to the neuroanesthesiologist and neurointensivist. Maintaining both the cardiovascular and cerebrovascular homeostasis are of paramount importance for a successful outcome. In this case report, we have mentioned the perioperative challenges of a 7-month-old baby with tetralogy of Fallot who underwent craniotomy and excision of choroid plexus papilloma.
A class of exactly solvable rationally extended non-central potentials in two and three dimensions
Authors: Kumari Nisha , Yadav Rajesh Kumar , Khare Avinash , Mandal Bhabani Prasad
Published: June 2018

We start from a seven parameter (six continuous and one discrete) family of non-central exactly solvable potentials in three dimensions and construct a wide class of ten parameters (six continuous and four discrete) family of rationally extended exactly solvable non-central real as well as PT symmetric complex potentials. The energy eigenvalues and the eigenfunctions of these extended non-central potentials are obtained explicitly and it is shown that the wave eigenfunctions of these potentials are either associated with the exceptional orthogonal polynomials or some type of new polynomials which can be further re-expressed in terms of the corresponding classical orthogonal polynomials. Similarly, we also construct a wide class of rationally extended exactly solvable non-central real as well as complex PT-invariant potentials in two dimensions.
Absorption and luminescence spectroscopy of mass-selected flavin adenine dinucleotide mono-anions
Authors: Giacomozzi L. , Kjær C. , Langeland Knudsen J. , Andersen L. H. , Brøndsted Nielsen S. , Stockett M. H.
Published: June 2018

We report the absorption profile of isolated Flavin Adenine Dinucleotide (FAD) mono-anions recorded using photo-induced dissociation action spectroscopy. In this charge state, one of the phosphoric acid groups is deprotonated and the chromophore itself is in its neutral oxidized state. These measurements cover the first four optical transitions of FAD with excitation energies from 2.3 to 6.0 eV (210–550 nm). The S0 → S2 transition is strongly blue shifted relative to aqueous solution, supporting the view that this transition has a significant charge-transfer character. The remaining bands are close to their solution-phase positions. This confirms that the large discrepancy between quantum chemical calculations of vertical transition energies and solution-phase band maxima cannot be explained by solvent effects. We also report the luminescence spectrum of FAD mono-anions in vacuo. The gas-phase Stokes shift for S1 is 3000 cm−1, which is considerably larger than any previously reported for other molecular ions and consistent with a significant displacement of the ground and excited state potential energy surfaces. Consideration of the vibronic structure is thus essential for simulating the absorption and luminescence spectra of flavins.
Two-dimensional displacement measurement based on two parallel gratings
Authors: Wei Peipei , Lu Xi , Qiao Decheng , Zou Limin , Huang Xiangdong , Tan Jiubin , Lu Zhengang
Published: June 2018

In this paper, a two-dimensional (2-D) planar encoder based on two parallel gratings, which includes a scanning grating and scale grating, is presented. The scanning grating is a combined transmission rectangular grating comprised of a 2-D grating located at the center and two one-dimensional (1-D) gratings located at the sides. The grating lines of the two 1-D gratings are perpendicular to each other and parallel with the 2-D grating lines. The scale grating is a 2-D reflective-type rectangular grating placed in parallel with the scanning grating, and there is an angular difference of 45° between the grating lines of the two 2-D gratings. With the special structural design of the scanning grating, the encoder can measure the 2-D displacement in the grating plane simultaneously, and the measured interference signals in the two directions are uncoupled. Moreover, by utilizing the scanning grating to modulate the phase of the interference signals instead of the prisms, the structure of the encoder is compact. Experiments were implemented, and the results demonstrate the validity of the 2-D planar grating encoder.
Interaction of argon and helium plasma jets and jets arrays with account for gravity
Authors: Babaeva Natalia Yu. , Naidis George V. , Panov Vladislav A. , Wang Ruixue , Zhao Yong , Shao Tao
Published: June 2018

In this paper, we discuss results from an experimental and computational study of the properties of a single jet and two-tube jet arrays operating in argon and helium. The jets are positioned horizontally. It was shown in experiments that the helium plasma plume bends upward and the plumes in the two-tubes jet array tend to divert due to the jet-jet interaction. To investigate these potential interactions, a computational study was performed of one- and two-tube argon and helium jet arrays having variable spacing. The effects of buoyancy forces on the jet-to-jet interaction of the plasma plumes are also investigated. Velocities of ionization waves inside and outside the tubes are estimated and compared for the argon and helium ionization waves. We show that in helium jet-jet interactions primarily depend on the spacing between the tubes and on the buoyancy forces. The helium plumes tend to merge into one single stream before dissipating, while the argon plasma plumes are less sensitive to the spacing of the jet tubes.
Positive ion temperature effect on the plasma-wall transition
Authors: Morales Crespo R.
Published: June 2018

This paper analyses the plasma-wall interaction of a plasma in contact with a conducting planar surface when the positive-ion temperature is not negligible compared with the electron one. The electric potential from the plasma to the wall is obtained by the appropriate formulation of the model as an initial-value problem as well as some features useful for experimental applications, such as the positive current-to-voltage characteristics, the saturation current density, the floating potential or an estimation of the sheath thickness. Finally, it is analysed how all these quantities depend on the ionization degree and the positive-ion temperature.
Influence of backflow on skin friction in turbulent pipe flow
Authors: Jalalabadi Razieh , Sung Hyung Jin
Published: June 2018

A direct numerical simulation of a turbulent pipe flow (Reτ = 544) is used to investigate the influence of the backflow on the vortical structures that contribute to the local skin friction. The backflow is a rare event with a probability density function (PDF) of less than 10−3. The backflow is found to extend up to y+ ≈ 4 and is induced by the presence of a vortex in the buffer layer. The flow statistics are conditionally sampled under the condition of a negative streamwise velocity (u < 0) at y+ = 3. The conditionally averaged u′v′+u<0 reaches its maximum at y+ ≈ 27. The intensified conditionally averaged velocity fluctuations contribute to vertical and spanwise momentum transport around the backflow. The ensemble averaged u′v′+ and u′w′+ reveal layered structures in the Q2 and Q4 events. A strong Q4 event appears above the backflow, flanked by two regions of Q2. The strong downwash of the flow along with the spanwise vortex induces the backflow. The upwash at upstream and downstream of the backflow enhances the movement of the low-speed flow in the streamwise and spanwise directions. The velocity–vorticity correlation reveals that the main contributions to Cf are the vorticity advection and vorticity stretching. The main contribution to the conditionally averaged Cf is the wall-normal gradient of the mean spanwise vorticity at the wall. The spanwise vorticity is positive above the backflow flanked by two regions of negative spanwise vorticity. The conditional PDF of the backflow under negative ul+ at y+ = 100 is more frequent than that under positive ul+.
Nonlinear evolution of energetic-particles-driven waves in collisionless plasmas
Authors: Li Shuhan , Liu Jinyuan , Wang Feng , Shen Wei , Li Dong
Published: June 2018

A one-dimensional electrostatic collisionless particle-in-cell code has been developed to study the nonlinear interaction between electrostatic waves and energetic particles (EPs). For a single wave, the results are clear and agree well with the existing theories. For coexisting two waves, although the mode nonlinear coupling between two wave fields is ignored, the second-order phase space islands can still exist between first-order islands generated by the two waves. However, the second-order phase islands are not formed by the superposed wave fields and the perturbed motions of EPs induced by the combined effect of two main resonances make these structures in phase space. Owing to these second-order islands, energy can be transferred between waves, even if the overlap of two main resonances never occurs. Depending on the distance between the main resonance islands in velocity space, the second-order island can affect the nonlinear dynamics and saturations of waves.
Structure and vibrational properties of the dominant O-H center in β-Ga2O3
Authors: Weiser Philip , Stavola Michael , Fowler W. Beall , Qin Ying , Pearton Stephen
Published: June 2018

Hydrogen has a strong influence on the electrical properties of transparent conducting oxides where it can give rise to shallow donors and can passivate deep compensating defects. We have carried out infrared absorption experiments on H- and D-doped β-Ga2O3 that involve temperature- and polarization-dependent effects as well as relative H- and D-concentrations to probe the defect structures that hydrogen can form. The results of analysis of these data, coupled with detailed theoretical calculations, show that the dominant O-H vibrational line observed at 3437 cm−1 for hydrogenated Ga2O3 is due to a relaxed VGa-2H center.
Ab initio intermolecular potential energy surface for the CO2—N2 system and related thermophysical properties
Authors: Crusius Johann-Philipp , Hellmann Robert , Castro-Palacio Juan Carlos , Vesovic Velisa
Published: June 2018

A four-dimensional potential energy surface (PES) for the interaction between a rigid carbon dioxide molecule and a rigid nitrogen molecule was constructed based on quantum-chemical ab initio calculations up to the coupled-cluster level with single, double, and perturbative triple excitations. Interaction energies for a total of 1893 points on the PES were calculated using the counterpoise-corrected supermolecular approach and basis sets of up to quintuple-zeta quality with bond functions. The interaction energies were extrapolated to the complete basis set limit, and an analytical site–site potential function with seven sites for carbon dioxide and five sites for nitrogen was fitted to the interaction energies. The CO2—N2 cross second virial coefficient as well as the dilute gas shear viscosity, thermal conductivity, and binary diffusion coefficient of CO2—N2 mixtures were calculated for temperatures up to 2000 K to validate the PES and to provide reliable reference values for these important properties. The calculated values are in very good agreement with the best experimental data.
Loss of USP28-mediated BRAF degradation drives resistance to RAF cancer therapies
Authors: Saei Azad , Palafox Marta , Benoukraf Touati , Kumari Nishi , Jaynes Patrick William , Iyengar Prasanna Vasudevan , Muñoz-Couselo Eva , Nuciforo Paolo , Cortés Javier , Nötzel Christopher , Kumarakulasinghe Nesaretnam Barr , Richard John Lalith Charles , Bin Adam Isa Zul Fazreen , Pang Brendan , Guzman Marta , Siqin Zhou , Yang Henry , Tam Wai Leong , Serra Violeta , Eichhorn Pieter Johan Adam
Published: June 2018

RAF kinase inhibitors are clinically active in patients with BRAF (V600E) mutant melanoma. However, rarely do tumors regress completely, with the majority of responses being short-lived. This is partially mediated through the loss of negative feedback loops after MAPK inhibition and reactivation of upstream signaling. Here, we demonstrate that the deubiquitinating enzyme USP28 functions through a feedback loop to destabilize RAF family members. Loss of USP28 stabilizes BRAF enhancing downstream MAPK activation and promotes resistance to RAF inhibitor therapy in culture and in vivo models. Importantly, we demonstrate that USP28 is deleted in a proportion of melanoma patients and may act as a biomarker for response to BRAF inhibitor therapy in patients. Furthermore, we identify Rigosertib as a possible therapeutic strategy for USP28-depleted tumors. Our results show that loss of USP28 enhances MAPK activity through the stabilization of RAF family members and is a key factor in BRAF inhibitor resistance.
Deglycosylation of Shaker KV channels affects voltage sensing and the open–closed transition
Authors: Lopez-Rodriguez Angelica , Holmgren Miguel
Published: June 2018

Most membrane proteins are subject to posttranslational glycosylation, which influences protein function, folding, solubility, stability, and trafficking. This modification has been proposed to protect proteins from proteolysis and modify protein–protein interactions. Voltage-activated ion channels are heavily glycosylated, which can result in up to 30% of the mature molecular mass being contributed by glycans. Normally, the functional consequences of glycosylation are assessed by comparing the function of fully glycosylated proteins with those in which glycosylation sites have been mutated or by expressing proteins in model cells lacking glycosylation enzymes. Here, we study the functional consequences of deglycosylation by PNGase F within the same population of voltage-activated potassium (KV) channels. We find that removal of sugar moieties has a small, but direct, influence on the voltage-sensing properties and final opening–closing transition of Shaker KV channels. Yet, we observe that the interactions of various ligands with different domains of the protein are not affected by deglycosylation. These results imply that the sugar mass attached to the voltage sensor neither represents a cargo for the dynamics of this domain nor imposes obstacles to the access of interacting molecules.
The SUMO-specific isopeptidase SENP2 is targeted to intracellular membranes via a predicted N-terminal amphipathic α-helix
Authors: Odeh Hana M. , Coyaud Etienne , Raught Brian , Matunis Michael J.
Published: June 2018

Sumoylation regulates a wide range of essential cellular functions, many of which are associated with activities in the nucleus. Although there is also emerging evidence for the involvement of the small ubiquitin-related modifier (SUMO) at intracellular membranes, the mechanisms by which sumoylation is regulated at membranes is largely unexplored. In this study, we report that the SUMO-specific isopeptidase, SENP2, uniquely associates with intracellular membranes. Using in vivo analyses and in vitro binding assays, we show that SENP2 is targeted to intracellular membranes via a predicted N-terminal amphipathic α-helix that promotes direct membrane binding. Furthermore, we demonstrate that SENP2 binding to intracellular membranes is regulated by interactions with the nuclear import receptor karyopherin-α. Consistent with membrane association, biotin identification (BioID) revealed interactions between SENP2 and endoplasmic reticulum, Golgi, and inner nuclear membrane-associated proteins. Collectively, our findings indicate that SENP2 binds to intracellular membranes where it interacts with membrane-associated proteins and has the potential to regulate their sumoylation and membrane-associated functions.
Modelling BioNano optical data and simulation study of genome map assembly
Authors: Chen Ping , Jing Xinyun , Ren Jian , Cao Han , Hao Pei , Li Xuan
Published: June 2018

Motivation

The launch of the BioNano next-generation mapping system has greatly enhanced the performance of physical map construction, thus rapidly expanding the application of optical mapping in genome research. Data biases have profound implications for downstream applications. However, very little is known about the properties and biases of BioNano data, and the very factors that contribute to whole-genome optical map assembly.

Results

We generated BioNano molecule data from eight organisms with diverse base compositions. We first characterized the properties/biases of BioNano molecule data, i.e. molecule length distribution, false labelling signal, variation of optical resolution and coverage distribution bias, and their inducing factors such as chimeric molecules, fragile sites and DNA molecule stretching. Second, we developed the BioNano Molecule SIMulator (BMSIM), a novel computer simulation program for optical data. BMSIM, is of great use for future genome mapping projects. Third, we evaluated the experimental variables that impact whole-genome optical map assembly. Specifically, the effects of coverage depth, molecule length, false-positive and false-negative labelling signals, chimeric molecules and nicking enzyme and nick site density were investigated. Our simulation study provides the empirical findings on how to control experimental variables and gauge analytical parameters to maximize benefit and minimize cost on whole-genome optical map assembly.

Availability and implementation

BMSIM is freely available on: https://github.com/pingchen09990102/BMSIM.

Supplementary information

Supplementary data are available at Bioinformatics online.
“Still a Cancer Patient”—Associations of Cancer Identity With Patient-Reported Outcomes and Health Care Use Among Cancer Survivors
Authors: Thong Melissa S Y , Wolschon Eva-Maria , Koch-Gallenkamp Lena , Waldmann Annika , Waldeyer-Sauerland Mechthild , Pritzkuleit Ron , Bertram Heike , Kajüter Hiltraud , Eberle Andrea , Holleczek Bernd , Zeissig Sylke R , Brenner Hermann , Arndt Volker
Published: June 2018

Background

The concept of cancer identity is gaining attention as more individuals are living with cancer as a chronic illness. Research is limited, and results suggest that a self-identity as “cancer patient” rather than a “cancer survivor” is associated with depression and lower health-related quality of life (HRQL). We aimed to identify factors associated with patient identity and investigate the associations between patient identity and treatment, health care use, psychosocial distress, and HRQL.

Methods

We used data from the population-based CAncEr Survivorship: A multi-Regional (CAESAR) study. Breast, colorectal, and prostate cancer survivors diagnosed during 1994–2004 completed a postal survey on patient identity, HRQL, psychological distress, and health care use in 2009–2011. We calculated odds ratios and the 95% confidence interval of having a patient identity. Analyses were adjusted for age, sex, education, and cancer stage, where appropriate.

Results

Of the 6057 respondents, colorectal cancer survivors (25%) were least likely to consider themselves patients, and prostate cancer survivors (36%) the most likely. Being male, younger age, comorbidity, higher cancer stage, and disease recurrence were associated with patient identity. Treatment was associated with patient identity, except among female colorectal cancer survivors. Having a patient identity was associated with higher health care use within the past 12 months. Survivors who still consider themselves patients were more likely to be depressed and reported significantly lower HRQL.

Conclusions

A significant proportion of cancer survivors still consider themselves patients five to 15 years postdiagnosis. Sensitivity to individuals’ self-identity should be considered when exploring their cancer experience.
The Politics of Pandemic Influenza Preparedness
Authors: Kamradt-Scott Adam
Published: June 2018

This chapter explores the ideas, concepts, norms, and agendas that have shaped the structures and actors governing the field of pandemic influenza preparedness. It begins by tracing the historical origins of the disease, then discusses the World Health Organization’s attempts to better respond to influenza pandemics through the development of biomedical knowledge and tools. The chapter then examines how, since the end of the Cold War, pandemic influenza has gained new prominence, in part as a result of its portrayal as a social, economic and political ‘threat’, which has prompted a transformation in the governance arrangements regarding the disease. The governance of pandemic influenza thus serves as a microcosm of the trends, actors, challenges and obstacles confronting global health governance more broadly.