bluuebunny/crossref_metadata_2025 huggingface dataset:
Interscopic fan travelscape: Hybridizing tourism through sport and art
Authors: Ziakas Vassilios , Tzanelli Rodanthi , Lundberg Christine
Published: May 2022

Contrary to the common compartmentalization of popular culture and events to specialized forms of fandom-induced tourism (e.g. film-, music-, sport-tourism), event-tourism spaces may also derive from blending different genres that enable symbiotic effects, for example, between sport and art. This paper provides a theoretical analysis of how event-tourism is interwoven and merged with sporting and cinematic popular culture, thereby creating a compound milieu for sport traveling aficionados that we name an “ Interscopic Fan Travelscape” (IFT). To ground our analysis, we use the example of a participatory sport event that blends organically sporting and cinematic facets of popular culture. This is a free-diving event, hosted in the Greek island of Amorgos, and commemorating the 1988 “Big Blue” film, which was primarily shot in Amorgos. Our conceptual framework provides a comprehensive understanding of composite popular culture settings and devoted fan-travel by integrating perspectives of neo-tribalism, serious leisure, fan pilgrimage and event-tourism.
Early childhood educators as language teachers: Preschool teachers’ understanding of language learning and language use
Authors: Cichocka Joanna
Published: May 2022

This article discusses a research study that involves five preschool teachers working in linguistically diverse classrooms. It focuses on how these teachers’ beliefs regarding language teaching and learning have emerged from their own experiences, and how they affect their understanding of their work. The study draws on the concept of plurilingualism and, to explore what the participants think, know and believe about language learning and language use, employs a dynamic and situated view of teacher cognition —that is, a view which pays particular attention to the specific context of teachers’ biographies and their emotional lives. Findings emerging from this research study suggest that, although teachers usually have numerous language learning experiences, their understanding of bilingualism is founded on monolingual assumptions, and, as a result, bilingualism is seen as complete fluency in both languages. In addition, the study proposes an extension of the current understanding of who a language teacher is by including early childhood educators in this conceptualization.
GC-MS based metabolite fingerprinting of serous ovarian carcinoma and benign ovarian tumor
Authors: Eroglu Evren Caglar , Kucukgoz Gulec Umran , Vardar Mehmet Ali , Paydas Semra
Published: May 2022

The aim of this study is to identify urinary metabolomic profile of benign and malign ovarian tumors patients. Samples were analyzed using gas chromatography-mass spectrometry (GC-MS) and metabolomic tools to define biomarkers that cause differentiation between groups. 7 metabolites were found to be different in patients with ovarian cancer (OC) and benign tumors (BT). R2Y and Q2 values were found to be 0.670 and 0.459, respectively. L-tyrosine, glycine, stearic acid, turanose and L-threonine metabolites were defined as prominent biomarkers. The sensitivity of the model was calculated as 90.72% and the specificity as 82.09%. In the pathway analysis, glutathione metabolism, aminoacyl-tRNA biosynthesis, glycine serine and threonine metabolic pathway, primary bile acid biosynthesis pathways were found to be important. According to the t-test, 29 metabolites were found to be significant in urine samples of OC patients and healthy controls (HC). R2Y and Q2 values were found to be 0.8170 and 0.749, respectively. These results showed that the model has high compatibility and predictive power. Benzoic acid, L-threonine, L-pyroglutamic acid, creatinine and 3,4-dihydroxyphenylacetic acid metabolites were determined as prominent biomarkers. The sensitivity of the model was calculated as 93.81% and the specificity as 98.59%. Glycine serine and threonine metabolic pathway, glutathione metabolism and aminoacyl-tRNA biosynthesis pathways were determined important in OC patients and HC. The R2Y, Q2, sensitivity and specificity values in the urine samples of BT patients and HC were found to be 0.869, 0.794, 91.75, 97.01% and 97.18%, respectively. L-threonine, L-pyroglutamic acid, benzoic acid, creatinine and pentadecanol metabolites were determined as prominent biomarkers. Valine, leucine and isoleucine biosynthesis and aminoacyl-tRNA biosynthesis were significant. In this study, thanks to the untargeted metabolomic approach and chemometric methods, every group was differentiated from the others and prominent biomarkers were determined.
Team reflexivity and the shared mind in interprofessional learning
Authors: Baerheim Anders , Ødegaard Elin E , Ness Ingunn Johanne
Published: May 2022

In interprofessional (IP) workplace education, course and project leaders need a deeper understanding of how students learn. Basically, in IP workplace learning students learn from each other, from the affected agents (patients, clients, children, youth, or elderly), from the staff, and from using multitudes of artifacts. Most of these interpersonal processes are largely tacit, and we therefor decided on elucidating their possible parts in IP learning theoretically, focusing on central interpersonal aspects of team learning processes; team reflexivity and intersubjectivity. Consequently, our study aim was to elucidate possible associations between team reflexivity and the shared mind when students in the IP team interact within each other, with the people and with the artifacts around them. In this article we investigate, elaborate, and conceptualize relevant social theories which address aspects of team reflexivity and intersubjective activities. We will then elaborate and conceptualize consequences for increased understanding IP team learning. Based on our mutual libraries, we have searched in PubMed and Google Scholar for team reflexivity, intersubjectivity, shared mind and their combinations. We came to understand reflexivity and team reflexivity as mostly tacit activities which may be regarded as being interpersonal and self-pacing. Intersubjectivity is however based on the free interaction between minds, orchestrating each other with common ideas, thoughts, attitudes, and bodily actions. Intersubjectivity is created when team members’ verbal and non-verbal activities resonate, giving a shared feeling of developing mutual and common ideas, concepts, and understanding. Team reflexivity, and intersubjectivity are necessary aspects in understanding the learning processes in IP student teams, and we have sketched some consequences for IP course design.
Differential expression profile of plasma exosomal microRNAs in chronic rhinosinusitis with nasal polyps
Authors: He Shuai , Wu Jun , Han Demin , Li Yunchuan , Wang Tong , Wei Hongzheng , Pan Yangwang , Zang Hongrui
Published: May 2022

Exosomes mediate inflammation and immune responses. The aim of the study was to examine the expression profiles of plasma exosomal microRNAs (miRNAs) and analyze their target gene functions in participants with chronic rhinosinusitis with nasal polyps (CRSwNP). We measured plasma exosomal miRNAs in five patients with CRSwNP and five controls. Transcripts per million (TPM) was used to assess miRNAs expression and the Benjamini–Hochberg procedure was employed for multiple comparisons correction. Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene ontology (GO) analyses revealed biological annotation and functional prediction of target genes. Compared with controls, we found that 159 exosomal miRNAs were differentially expressed by miRNA sequencing in CRSwNP. The top three upregulated miRNAs were novel_miR_677, novel_miR_1037, and novel_miR_79, while the top three downregulated miRNAs were novel_miR_192, novel_miR_1022, and novel_miR_4. The target functions in the GO and KEGG analyses included axon guidance, extracellular matrix (ECM)–receptor interaction, protein digestion and absorption, the calcium, the Hippo, the Notch, the ErbB, the cAMP signaling pathway, and focal adhesion. This study describes the dissection of plasma exosomal miRNA profiling in CRSwNP. Our findings may provide a certain basis for further mechanism research and exploration of diagnostic values.
To Dean, or Not To Dean?
Authors: Baker Sarah J. , Osanloo Azadeh F.
Published: May 2022

The lack of women holding high-level leadership positions in higher education institutions is problematic. From a historical standpoint, women face several more obstacles while working toward obtaining leadership roles in higher education than men do. In addition, from a societal lens, women are judged differently in regard to leadership style, emotion, and success in higher education leadership. This case follows a woman who obtained the position of interim dean and was unceremoniously removed from the role. Authors make suggestions regarding factors for her success, how her emotions affected her performance, and variables leading to her accepting the role of interim dean.
An Investigation of Google Searches for Knee Osteoarthritis and Stem Cell Therapy: What are Patients Searching Online?
Authors: Sullivan Breanna , Platt Brooks , Joiner Josh , Jacobs Cale , Conley Caitlin , Landy David C. , Stone Austin V.
Published: May 2022

Background:

Stem cell therapies have emerged as a potential treatment for osteoarthritis (OA), but their role remains controversial. Their growing popularity may lead patients to search for information online, but search queries and sources are unknown.

Purpose:

We sought to (1) identify the most frequently asked questions on Google related to knee OA and stem cell therapy, (2) assess the types of websites accessed through these searches, and (3) evaluate the transparency of these websites.

Methods:

The search terms “knee,” “osteoarthritis,” and “stem cell” were entered into Google. Frequently asked questions suggested by Google, and their corresponding websites were extracted. Questions and websites were classified by type. Journal of the American Medical Association ( JAMA) benchmark criteria were used as a measure of website transparency (range: 0–4). Analysis of variance tests were performed to compare JAMA benchmark criteria between website types.

Results:

Of the 145 questions and website extracted, the most common question subtype pertained to non-stem cell treatments (41.4%) for knee OA; the most common website types were medical publishers (24.8%) and nonacademic medical practices (22.8%). JAMA scores were highest for medical publishers (mean: 3.81) and lowest for nonacademic medical practices (mean: 1.55). Nonacademic medical practices had significantly worse JAMA scores compared with commercial, academic, and medical publishers.

Conclusion:

The most frequent questions pertained to non-stem cell treatments, although questions related to stem cell efficacy were most common among the 10 most frequent questions. Google searches on knee OA and stem cell therapies often directed the user to websites lacking transparency and verifiable information. Further research is warranted.
Gender, Police Culture, and Structured Ambivalence: Navigating ‘Fit' with the Brotherhood, Boys’ Club, and Sisterhood
Authors: Sanders Carrie B. , Gouweloos Julie , Langan Debra
Published: May 2022

Women are increasingly represented in policing; however, inclusion alone will not eradicate existing structural and cultural barriers to meaningful change. Insights from interviews with ninety-one Canadian women police of varied rank and tenure, demonstrate women’s experiences of structured ambivalence as they strategically deploy and resist gendered policing narratives of the Brotherhood, Boys’ Club, and Sisterhood to negotiate their own ‘fit.’ In this way, they both challenge and reinforce gendered boundaries that create barriers to meaningful transformation. These findings demonstrate the need for change initiatives to address the complex and ever-shifting role of gender in policing organizations.
Use of Plain Radiography of Uninjured Wrists as Patient-Specific Markers of Successful Reduction of Unilateral Distal Radius Fractures
Authors: Hwang Lyahn K. , Greige Nicolas , Wang Fei , Yi Joseph K. , Treiser Matthew D. , Ricci Joseph A.
Published: May 2022

Background:

The standard of care of distal radius fractures requiring operative intervention involves restoration of anatomical alignment radiologically by comparing preoperative films and intraoperative fluoroscopy with established values based on population norms. The objective of this study is to evaluate the use of plain radiographs obtained from the uninjured wrist of patients who present with unilateral displaced distal radius fractures as a measure of successful achievement of anatomical realignment.

Methods:

A retrospective review was performed on 133 consecutive patients who presented from August 2020 to August 2021 with a diagnosis of unilateral distal radius fracture as confirmed on 3-view plain radiography. Patients who had bilateral radiographs and underwent open reduction and internal fixation were included. The primary outcome measure was comparison of radial inclination, radial height, tilt, and ulnar variance measured by 3 observers on preoperative, 1-week postoperative, and uninjured contralateral wrist films.

Results:

Twenty-one patients were included for analysis. Comparison of postoperative radiologic parameters with the contralateral uninjured extremity revealed a mean radial inclination difference of 3.8°, radial height difference of 2.0 mm, volar tilt difference of 6.3°, and ulnar variance difference of 0.9 mm. The average postreduction radial height was found to deviate from contralateral radial height significantly more than from the historic radial height parameter (2.0 vs 0.6 mm, P < .001).

Conclusion:

Attempts at achieving distal radius fracture reduction to within historical normal limits may result in an increased deviation from patient-specific anatomical parameters, especially with respect to radial height.
Gunshots, Sociability and Community Defence. Shooting Associations in Imperial Germany and its Colonies
Authors: Camilleri Nicola
Published: May 2022

Shooting associations represented one of the most popular expressions of sociability in Imperial Germany. Their club houses were to be found in large and medium-sized towns, in villages, and in overseas colonies, too. Middle class men would regularly gather to practice shooting and to organize competitions, activities characterized by clearly gendered rituals of social life. Based on values of loyalty to the Emperor and to fellow members, association life closely reflected the ideological agenda of the protestant Kaiserreich. Their popularity and pervasiveness earned shooting associations a place in George Mosse's groundbreaking work on the nationalization of the masses. Nevertheless, they have been mostly neglected in research on bourgeois sociability and on militarism. This article is the first scholarly attempt to study this form of associationism in Imperial Germany and its colonies. Having developed out of the old tradition of civic militias, shooting societies lost their primary policing and military function during the 19th century. However, community defence remained an essential task, which was viewed then as a moral and civil, rather than military, matter. The article examines the cultural and social aspects of shooting societies and relates this form of associationism to wider issues of military culture in the Kaiserreich.
Tangential mechanical properties analysis of cylindric elastoplastic contact model
Authors: Guo Tieneng , Ji Ruguo , Ma Yunfei , Hua Xu , Peng Liwei
Published: May 2022

An elastoplastic contact model of two cylinders is proposed in this paper. The crossing angle of two cylinders axes is introduced into the contact model. An analytical solution including the tangential displacement and tangential load under the axis crossing angles is provided. The finite element method is used to verify analytical solutions. The verification result shows that the analytical solution is in good agreement with the simulation result obtained by the finite element analysis. The stress distribution law of the cylinder contact surface is analyzed based on the contact model. The influence of model parameters on tangential mechanical properties is explored. Results show that tangential mechanical properties of the cylinder are governed by many factors, such as the applied load, material properties, and axis crossing angle. Cylinder contact models with different axis crossing angles can be applied to some engineering structures, such as roller bearings and gear meshing.
Experimental comparison on condensation and evaporation outside double-side enhanced horizontal tubes
Authors: Cheng Jian , Tao Le-Ren , Huang Jia-Yu , Zhou Zhuo , Liu Peng-Peng
Published: May 2022

Experimental investigations of the condensation and evaporation of R410A on the outside of two double-side enhanced heat transfer tubes were conducted, and the tube performance was compared with that of a smooth copper tube. The research object consisted of a smooth tube with a length of 2000 mm and an outer diameter of 25.4 mm and two double-side enhanced tubes of the same size. The evaporation experiments were run under the following conditions: the evaporation saturation temperature was 7°C, water inlet temperature was 8°C–18°C, and water flow rate was 0.6–1.6 m3/h. The condensation experiments were run under the following conditions: the saturation temperature was 35°C, water inlet temperature was 21°C–30°C, and water flow rate was 0.4–1.6 m3/h. During the data processing, the heat transfer coefficient of the water side was obtained using the Gnielinski-Wilson method, and that of the refrigerant side was obtained using the thermal resistance separation method. The effect of the surface structure on the heat transfer performance was analyzed, and a strengthening measure was proposed. The experimental results indicate that the evaporation heat transfer coefficients outside the smooth tube increased with the raise of the inlet water temperature, while the double-side enhanced tubes decreased; both the condensation heat transfer coefficients outside the smooth tube and the enhanced tubes increased with the increase of the water flow rate. Due to the different type of enhanced tube ribs, tube #3 surface structure is more conducive to the formation of vaporization core, which is about 30% higher than the outer heat transfer coefficient of tube #2. It is more in line with the requirements of strengthening horizontal single tube external pool boiling heat transfer. In the condensation experiment, the condensation heat transfer coefficients outside the smooth tube increased with the raise of the inlet water temperature, while the double enhanced tubes decreased; both the condensation heat transfer coefficients outside the smooth tube and the enhanced tubes increased with the increase of the water flow rate. With the analysis of the rib structure, the outer surface structure of tube #2 has stronger drainage capacity, and the external average condensation heat transfer coefficient is about 7% higher than that of tube #3, which is more in line with the requirements of condensing heat transfer outside the single tube.
Jiedu Huoxue Decoction for Cytokine Storm and Thrombosis in Severe COVID-19: A Combined Bioinformatics and Computational Chemistry Approach
Authors: Liu Ying , Yan Han , Jia Hui-bin , Pan Li , Liu Jia-zheng , Zhang Ya-wen , Wang Jing , Qin Dao-gang , Ma Lei , Wang Ting
Published: May 2022

Jiedu Huoxue Decoction (JHD), a recommended traditional prescription for patients with severe COVID-19, has appeared in the treatment protocols in China. Based on bioinformatics and computational chemistry methods, including molecular docking, molecular dynamics (MD) simulation, and Molecular Mechanics Generalized Born Surface Area (MM/GBSA) calculation, we aimed to reveal the mechanism of JHD in treating severe COVID-19. The compounds in JHD were obtained and screened on TCMSP, SwissADME, and ADMETLab platforms. The compound targets were obtained from TCMSP and STITCH, while COVID-19 targets were obtained from Genecards and NCBI. The protein-protein interaction network was constructed by using STRING. Gene Ontology (GO) and KEGG enrichment were performed with ClueGO and R language. AutoDock vina was employed for molecular docking. 100 ns MD simulation of the optimal docking complex was carried out with AmberTools 20. A total of 84 compounds and 29 potential targets of JHD for COVID-19 were collected. The key phytochemicals included quercetin, luteolin, β-sitosterol, puerarin, stigmasterol, kaempferol, and wogonin, which could regulate the immune system. The hub genes included IL6, IL10, VEGFA, IL1B, CCL2, HMOX1, DPP4, and ACE2. ACE2 and DPP4 were related to SARS-CoV-2 entering cells. GO and KEGG analysis showed that JHD could intervene in cytokine storm and endothelial proliferation and migration related to thrombosis. The molecular docking, 100 ns MD simulation, and MM/GBSA calculation confirmed that targets enriched in the COVID-19 pathway had high affinities with related compounds, and the conformations of the puerarin-ACE2, quercetin-EGFR, luteolin-EGFR, and quercetin-IL1B complexes were stable. In a word, JHD could treat COVID-19 by intervening in cytokine storm, thrombosis, and the entry of SARS-CoV-2, while regulating the immune system. These mechanisms were consistent with JHD's therapeutic concept of “detoxification” and “promoting blood circulation and removing blood stasis” in treating COVID-19. The research provides a theoretical basis for the development and application of JHD.
Unusual imaging characteristics of cystic meningioma in cerebellopontine angle
Authors: Adachi Saki , Yamashita Koji , Nakamizo Akira , Amano Toshiyuki , Momosaki Seiya , Noguchi Tomoyuki
Published: May 2022

We report a case of cystic meningioma at the left cerebellopontine angle (CPA). Magnetic resonance imaging demonstrated both solid and cystic components in the tumor. The cystic component appeared slightly hyperintense compared to cerebrospinal fluid on fluid-attenuated inversion recovery (FLAIR) imaging. A hypointense tubular structure was identified in the cystic component on 3D driven equilibrium sequencing. These imaging findings are unusual for cystic meningioma. However, awareness of these unusual imaging features is important to determine appropriate treatment strategies although cystic meningioma at the CPA is extremely rare.
Study on physical behaviors according to element formulations of ballistic impact simulation models
Authors: Yoo Tae Kwang
Published: May 2022

During modeling ballistic impact, physical behaviors were investigated according to element formulations. In order to conduct the investigation, a simple ballistic impact simulation model was used. Several case studies consisting of FEM (Finite Element Method) and SPH (Smoothed Particle Hydrodynamics) models were conducted using the LS-DYNA research/commercial code. As a result, these case studies suggest that the SPH formulation is effective to simulate interactions among particles after failures so that the penetration phenomenon of the projectile were well described. In addition, some parameters relating to the SPH formulation such as particle spacing and smoothing length constant were investigated. Finally, for the verification, 20 mm fragment simulating projectile tests to verify the protection performance of K9 Thunder grill louver were compared with the simulations applying the FEM and SPH formulations.
The material consequences of “chipification”: The case of software-embedded cars
Authors: Forelle MC
Published: May 2022

Today's modern car is an assemblage of mechanical and digital components, of metal panels that comprise its structure and silicon chips that run its functions. Communication and information studies scholars have interrogated the problematic aspects of the programs that run those functions, revealing serious issues surrounding privacy and security, worker surveillance, and racial, gendered, and class-based bias. This article contributes to that work by taking a step back and asking about the issues inherent not in the software running on these chips, but on the microchips themselves. Using the lens of “chipification”—the process by which a device is rendered capable of reading and processing data through the embedding of microchips—this article explores how the integration of microchips also involves processes that are often borne from, replicate, or create troubling power dynamics of their own. It takes light-duty passenger vehicles as a case study into how chipification is radically reshaping such processes as resource allocation, labor flows, and cultural practices around car manufacture, use, repair, and modification. By naming the process of chipification, this article allows researchers to identify and analyze the ways that integrating data processing capabilities into everyday devices is not a frictionless practice, but rather one that implicates a variety of power dynamics within these massive industries.
Registered nurses’ experiences of challenging situations in their psychosocial working environment in home care of patients with functional and/or intellectual disability: a qualitative study
Authors: Ericson Lidman Eva , Antonsson Helena
Published: May 2022

Around 15% of the world's population lives with some form of functional and/or intellectual disability (FID). Nursing staff often face threats and violence in the care of patients with FID. This study aimed to illuminate registered nurses’ experiences of challenging situations in their psychosocial working environment in home care of patients with functional and/or intellectual disabilities. Nine registered nurses/district nurses working in municipal settings were interviewed using a qualitative approach. COREQ was used for explicit and comprehensive reporting of qualitative studies. Three categories were revealed: To face challenging behaviour, To face emotional burden and To face communication difficulties. Nurses need continuous training and support to cope with work-related stress to meet a person with FID in a calm and relaxed mood and to be prepared for critical situations in their psychosocial environment. Training and support may enable nurses to be in a state of harmony and to be present in order to build caring relationships.
Cross-National News Narratives of the Paralympic Games: Computational Text Analysis of the Media Coverage in the United States and South Korea
Authors: Yoo Joseph J. , Min Bumgi , Koh YoungHwan
Published: May 2022

This study presents a comparison of themes found in the U.S. and South Korean news coverage of the Paralympics. It is commonplace to associate rugged individualism with the former country and collectivism with the latter, and we assumed that these national traits would be apparent in the news coverage of the Paralympic Games in the two countries. Using the keyword “Paralympics,” we searched two decades of newspaper coverage and performed latent Dirichlet allocation (LDA) topic modeling on the articles that the search produced. The results of the modeling confirmed that the reporting in each country reflected its national characteristics, with the U.S. coverage reinforcing ableism and the South Korean coverage focusing on the government’s efforts to promote large-scale sporting events. The news stories in both countries were alike, though, in treating disabled athletes and traditional athletes differently. Part of the contribution of this research to the academic study of disabled athletes is the use of computational textual analysis to address these issues.
Nuclear Interactions: A Spotlight on Nuclear Mitochondrial Membrane Contact Sites
Authors: Ovciarikova Jana , Shikha Shikha , Sheiner Lilach
Published: May 2022

Membrane contact sites (MCS) are critical for cellular functions of eukaryotes, as they enable communication and exchange between organelles. Research over the last decade unravelled the function and composition of MCS between a variety of organelles including mitochondria, ER, plasma membrane, lysosomes, lipid droplets, peroxisome and endosome, to name a few. In fact, MCS are found between any pair of organelles studied to date, with common functions including lipid exchange, calcium signalling and organelle positioning in the cell. Work in the past year has started addressing the composition and function of nuclear-mitochondrial MCS. Tether components mediating these contacts in yeast have been identified via comprehensive phenotypic screens, which also revealed a possible link between this contact and phosphatidylcholine metabolism. In human cells, and in the protozoan parasites causing malaria, proximity between these organelles is proposed to promote cell survival via a mitochondrial retrograde response. These pioneering studies should inspire the field to explore what cellular processes depend on the exchange between the nucleus and the mitochondrion, given that they play such central roles in cell biology.
Superior Capsular Reconstruction for Irreparable Rotator Cuff Tears: Technique and Associated Considerations
Authors: Kerzner Benjamin , Swindell Hasani W. , Dasari Suhas P. , Khan Zeeshan A. , Chahla Jorge , Verma Nikhil N.
Published: May 2022

Background:

Massive rotator cuff tears lead to superior humeral head translation and may prevent successful repair. Superior capsular reconstruction (SCR) can overcome this by improving the compression and depressor effects of the rotator cuff and joint capsule simultaneously.

Indications:

SCR is indicated in young, active patients with massive rotator cuff tears or a functional cuff deficiency from a failed previous rotator cuff repair without significant arthritis, articulation of the humeral head with the acromion, or dysfunctional deltoid.

Technique Description:

During the procedure, repair of the subscapularis is performed using a polydioxanone (PDS) suture shuttling device to pass suture through the subscapularis in an inverted mattress knotless configuration. Posterior infraspinatus tendon fibers are reduced and the tuberosity is abraded and decorticated followed by completion of the partial repair of the posterior cuff. Anchors are placed on the glenoid and articular margins followed by preparation of the dermal allograft with luggage tags medially and perforation laterally. The graft is passed and secured in a double row configuration laterally with apposition of the graft to the remnant, repaired rotator cuff posteriorly.

Results:

Passive range of motion (ROM) is begun at week 4, followed by progressive active ROM at weeks 6 to 12, and advancement of full ROM and advanced strengthening begun at 3 months. With SCR, a patient can expect maximal strength and function to be regained at 1 year postoperatively. Significant clinical improvement following SCR has been seen in visual analog scale (VAS) and American Shoulder and Elbow Surgeons Shoulder Score (ASES) scores for most patients, although there is a varied degree of return to sport following this procedure.

Discussion/Conclusion:

Clinical evidence suggests that SCR is a beneficial therapeutic intervention for short to medium term outcomes in younger patients with massive, irreparable rotator cuff tears. More studies are needed to identify the optimal thickness of the graft.
Method for Fault Feature Selection for a Baler Gearbox Based on an Improved Adaptive Genetic Algorithm
Authors: Ren Bin , Bai Dong , Xue Zhanpu , Xie Hu , Zhang Hao
Published: May 2022

The performance and efficiency of a baler deteriorate as a result of gearbox failure. One way to overcome this challenge is to select appropriate fault feature parameters for fault diagnosis and monitoring gearboxes. This paper proposes a fault feature selection method using an improved adaptive genetic algorithm for a baler gearbox. This method directly obtains the minimum fault feature parameter set that is most sensitive to fault features through attribute reduction. The main benefit of the improved adaptive genetic algorithm is its excellent performance in terms of the efficiency of attribute reduction without requiring prior information. Therefore, this method should be capable of timely diagnosis and monitoring. Experimental validation was performed and promising findings highlighting the relationship between diagnosis results and faults were obtained. The results indicate that when using the improved genetic algorithm to reduce 12 fault characteristic parameters to three without a priori information, 100% fault diagnosis accuracy can be achieved based on these fault characteristics and the time required for fault feature parameter selection using the improved genetic algorithm is reduced by half compared to traditional methods. The proposed method provides important insights into the instant fault diagnosis and fault monitoring of mechanical devices.
Integrative analysis of circRNA, miRNA, and mRNA profiles to reveal ceRNA regulation in chicken muscle development from the embryonic to post-hatching periods
Authors: Lei Qiuxia , Hu Xin , Han Haixia , Wang Jie , Liu Wei , Zhou Yan , Cao Dingguo , Li Fuwei , Liu Jie
Published: May 2022

Background

The growth and development of skeletal muscle are regulated by protein-coding genes and non-coding RNA. Circular RNA (circRNA) is a type of non-coding RNA involved in a variety of biological processes, especially in post-transcriptional regulation. To better understand the regulatory mechanism of circRNAs during the development of muscle in chicken, we performed RNA-seq with linear RNA depletion for chicken breast muscle in 12 (E 12) and17 (E 17) day embryos, and 1 (D 1), 14 (D 14), 56 (D 56), and 98 (D 98) days post-hatch.

Results

We identified 5755 differentially expressed (DE)-circRNAs during muscle development. We profiled the expression of DE-circRNAs and mRNAs (identified in our previous study) at up to six time points during chicken muscle development and uncovered a significant profile (profile 16) for circRNA upregulation during aging in muscle tissues. To investigate competing endogenous RNA (ceRNA) regulation in muscle and identify muscle-related circRNAs, we constructed a circRNA-miRNA-mRNA regulatory network using the circRNAs and mRNAs from profile 16 and miRNAs identified in our previous study, which included 361 miRNAs, 68 circRNAs, 599 mRNAs, and 31,063 interacting pairs. Functional annotation showed that upregulated circRNAs might contribute to glycolysis/gluconeogenesis, biosynthesis of amino acids, pyruvate metabolism, carbon metabolism, glycogen and sucrose metabolism through the ceRNA network, and thus affected postnatal muscle development by regulating muscle protein deposition. Of them, circRNA225 and circRNA226 from the same host gene might be key circRNAs that could regulate muscle development by interacting with seven common miRNAs and 207 mRNAs. Our experiments also demonstrated that there were interactions among circRNA225, gga-miR-1306-5p, and heat shock protein alpha 8 (HSPA8).

Conclusions

Our results suggest that adequate supply of nutrients such as energy and protein after hatching may be a key factor in ensuring chicken yield, and provide several candidate circRNAs for future studies concerning ceRNA regulation during chicken muscle development.
Rice (Oryza sativa L.) cytochrome P450 protein 716A subfamily CYP716A16 regulates disease resistance
Authors: Wang Aijun , Ma Li , Shu Xinyue , Jiang Yuqi , Liang Juan , Zheng Aiping
Published: May 2022

Background

The sustainable development of rice production is facing severe threats by a variety of pathogens, such as necrotrophicRhizoctonia solaniand hemibiotrophicXanthomonas oryzaepv. oryzae (Xoo). Mining and applying resistance genes to increase the durable resistance of rice is an effective method that can be used to control these diseases.

Results

In this research, we isolated and characterized CYP716A16, which is a positive regulator of rice toR. solaniAG1-IA andXoo, and belongs to the cytochrome P450 (CYP450) protein 716A subfamily. Overexpression (OE) ofCYP716A16resulted in enhanced resistance toR. solaniAG1-IA andXoo, while RNA interference (RNAi) ofCYP716A16resulted in increased susceptibility compared with wild-type (WT) plants. Additionally, jasmonic acid (JA)-dependent defense responses and reactive oxygen species (ROS) were activated in theCYP716A16-OE lines afterR. solaniAG1-IA inoculation. The comparative transcriptomic and metabolomics analysis of CYP716A16-OE and the WT lines showed that OE ofCYP716A16activated the biosynthesis of flavonoids and increased the amounts of narcissoside, methylophiopogonanone A, oroxin A, and amentoflavone in plants.

Conclusion

Based on these results, we suggest that JA-dependent response, ROS level, multiple resistance-related proteins, and flavonoid contents play an important role inCYP716A16-regulatedR. solaniAG1-IA andXooresistance. Our results broaden our knowledge regarding the function of a P450 protein 716A subfamily in disease resistance and provide new insight into the molecular mechanism of rice immune response.
The evaluation of levosimendan in patients with acute myocardial infarction related ventricular septal rupture undergoing cardiac surgery: a prospective observational cohort study with propensity score analysis
Authors: Li Ze-Shi , Wang Kuo , Pan Tuo , Sun Yan-Hua , Liu Chang , Cheng Yong-Qing , Zhang He , Zhang Hai-Tao , Wang Dong-Jin , Chen Zu-Jun
Published: May 2022

Study objective

The purpose of the present study was to evaluate the efficacy of levosimendan in patients with acute myocardial infarction related ventricular septal rupture (AMI-VSR) underwent cardiac surgery.

Design

Prospective observational cohort study with propensity score analysis.

Patients

There were 261 patients with AMI-VSR in our study. After 1:1 propensity matching, 106 patients (53 levosimendan and 53 control) were selected in the matched cohort.

Interventions

None.

Measurements

Patients who received levosimendan were assigned to the levosimendan group (n = 164). The patients who were not received were levosimendan assigned to the control group (n = 97). The levosimendan was initiated immediately after cardiopulmonary bypass. Then, it has been maintained during the postoperative 3 days. The poor outcomes were identified as follows: death and postoperative complications (postoperative stroke, low cardiac output syndromeneeded mechanical circulatory support after surgery, acute kidney injury (≥ stage III), postoperative infection or septic shock, new developed atrial fibrillation or ventricular arrhythmias).

Main results

Before matching, the control group had more length of ICU stay (6.69 ± 3.90 d vs. 5.20 ± 2.24 d,p < 0.001) and longer mechanical ventilation time (23 h, IQR: 16–53 h vs. 16 h, IQR: 11–23 h,p < 0.001). Other postoperative outcomes have not shown significant differences between two groups. After matching, no significant difference was found between both groups for all postoperative outcomes. The Kaplan–Meier survivul estimate and log-rank test showed that the 90-day survival had no significant differences between two groups before and after matching.

Conclusion

Our study found that a low-dose infusion of levosimendan in AMI-VSR patients underwent surgical repair did not associated with positively affect to postoperative outcomes.
Postoperative pulmonary complications with adjuvant regional anesthesia versus general anesthesia alone: a sub-analysis of the Perioperative Research Network study
Authors: Bartels Karsten , Frendl Gyorgy , Sprung Juraj , Weingarten Toby N. , Subramaniam Balachundhar , Martinez Ruiz Ricardo , Lee Jae-Woo , Henderson William G. , Moss Angela , Sodickson Alissa , Giquel Jadelis , Vidal Melo Marcos Francisco , Fernandez-Bustamante Ana ,   for the Perioperative Research Network (PRN) investigators, Amar David , Bartels Karsten , Blum James , Chen Lee-Lynn , Desiderio Dawn , Douin David Josh , Fernandez-Bustamante Ana , Eikermann Matthias , Frendl Gyorgy , Giquel Jadelis , Grecu Loreta , Gupta Ravindra Alok , Karan Suzanne , Kor Daryl J. , Lee Jae-Woo , Martinez Ruiz Ricardo , Musch Guido , Sprung Juraj , Subramaniam Balachundhar , Sodickson Alissa , Tanaka Pedro , Vidal Melo Marcos Francisco , Wanderer Jonathan P. , Weingarten Toby N.
Published: May 2022

Background

Adjuvant regional anesthesia is often selected for patients or procedures with high risk of pulmonary complications after general anesthesia. The benefit of adjuvant regional anesthesia to reduce postoperative pulmonary complications remains uncertain. In a prospective observational multicenter study, patients scheduled for non-cardiothoracic surgery with at least one postoperative pulmonary complication surprisingly received adjuvant regional anesthesia more frequently than those with no complications. We hypothesized that, after adjusting for surgical and patient complexity variables, the incidence of postoperative pulmonary complications would not be associated with adjuvant regional anesthesia.

Methods

We performed a secondary analysis of a prospective observational multicenter study including 1202 American Society of Anesthesiologists physical status 3 patients undergoing non-cardiothoracic surgery. Patients were classified as receiving either adjuvant regional anesthesia or general anesthesia alone. Predefined pulmonary complications within the first seven postoperative days were prospectively identified. Groups were compared using bivariable and multivariable hierarchical logistic regression analyses for the outcome of at least one postoperative pulmonary complication.

Results

Adjuvant regional anesthesia was performed in 266 (22.1%) patients and not performed in 936 (77.9%). The incidence of postoperative pulmonary complications was greater in patients receiving adjuvant regional anesthesia (42.1%) than in patients without it (30.9%) (site adjusted p = 0.007), but this association was not confirmed after adjusting for covariates (adjusted OR 1.37; 95% CI, 0.83–2.25; p = 0.165).

Conclusion

After adjusting for surgical and patient complexity, adjuvant regional anesthesia versus general anesthesia alone was not associated with a greater incidence of postoperative pulmonary complications in this multicenter cohort of non-cardiothoracic surgery patients.
CircRNA circ-ATAD1 suppresses miR-618 maturation to participate in colorectal cancer
Authors: Cao Li , Dong Guanglong , Li Huan
Published: May 2022

Background

CircRNA circ-ATAD1 has been characterized as an oncogenic circRNA in gastric cancer, while its role in colorectal cancer (CRC) is unknown. This study was carried out to explore the role of circ-ATAD1 in CRC.

Methods

Paired CRC and adjacent non-tumor tissue samples collected from 64 CRC patients were subjected to RNA extractions and RT-qPCRs to analyze the expression of circ-ATAD1, premature miR-618, and mature miR-618 in CRC. The effects of circ-ATAD1 overexpression on miR-618 maturation were analyzed by transfecting circ-ATAD1 expression vector into CRC cells, followed by determining the expression of premature miR-618 and mature miR-618 using RT-qPCR. The subcellular location of circ-ATAD1 was analyzed by nuclear fractionation assay, and the interaction between circ-ATAD1 and premature miR-618 was analyzed by RNA pull-down assay. The roles of circ-ATAD1, premature miR-618, and mature miR-618 in regulating CRC cell proliferation were explored by CCK-8 assay.

Results

Circ-ATAD1 was upregulated in CRC and predicted poor survival. In addition, circ-ATAD1 was inversely correlated with mature miR-618 but not premature miR-618. In CRC cells, circ-ATAD1 overexpression decreased the level of mature miR-618 but not premature miR-618. Circ-ATAD1 was detected in both the nucleus and cytoplasm. A direct interaction between circ-ATAD1 and miR-618 was observed. Moreover, circ-ATAD1 overexpression reduced the inhibitory effects of miR-618 overexpression on cell proliferation.

Conclusion

Circ-ATAD1 is overexpressed in CRC and may suppress miR-618 maturation to participate in CRC.
Myasthenia gravis coexisting with HINT1-related motor axonal neuropathy without neuromyotonia: a case report
Authors: Fang Jia , Huang Hui , Lei Qiang , Luo Yingying , Tang Zhengchu , Shi Xiaoliu , Tang Jian Guang
Published: May 2022

Background

HINT1 mutations cause an autosomal recessive axonal neuropathy with neuromyotonia. This is a first case report of coexistence of myasthenia gravis (MG) and HINT1-related motor axonal neuropathy without neuromyotonia.

Case presentation

A 32-year-old woman presented with recurrent ptosis for 8 years, diplopia for 2 years and limb weakness for 1 year and a half. Neostigmine test, elevated AChR antibody level and positive repetitive nerve stimulation supported the diagnosis of MG. Electroneurography (ENG) and electromyography (EMG) examinations revealed a motor axonal neuropathy without neuromyotonic or myokymic discharges. Next-generation sequencing and Sanger sequencing were performed to identify the gene responsible for suspected hereditary neuropathy. Genetic testing for a HINT1 mutation was performed and revealed a homozygous mutation at c.278G>T (p. G93V). The patient was treated with pyridostigmine, oral prednisolone and azathioprine. Her ptosis and diplopia have significantly improved at 6-month follow-up.

Conclusions

Concurrence of MG and hereditary motor axonal neuropathy without neuromyotonia is quite rare. Detection of ptosis with or without ophthalmoplegia, distribution of limb weakness, and reflex can help in recognizing the combination of MG and peripheral neuropathy. Early diagnosis is important for initial treatment and prognosis. The novel homozygous variant c.278G>T(p.G93V) contributes to the pathogenic variants spectrum of the HINT1 gene.
Biological networks in gestational diabetes mellitus: insights into the mechanism of crosstalk between long non-coding RNA and N6-methyladenine modification
Authors: Du Runyu , Bai Yu , Li Ling
Published: May 2022

Background

Gestational diabetes mellitus (GDM) is one of the most common complications of pregnancy. The mechanism underlying the crosstalk between long non-coding RNAs (lncRNAs) and N6-methyladenine (m6A) modification in GDM remain unclear.

Methods

We generated a lncRNA-mediated competitive endogenous RNA (ceRNA) network using comprehensive data from the Gene Expression Omnibus database, published data, and our preliminary findings. m6A-related lncRNAs were identified based on Pearson correlation coefficient (PCC) analysis using our previous profiles. An integrated pipeline was established to constructed a m6A-related subnetwork thereby predicting the potential effects of the m6A-related lncRNAs.

Results

The ceRNA network was composed of 16 lncRNAs, 17 microRNAs, 184 mRNAs, and 338 edges. Analysis with the Kyoto Encyclopedia of Genes and Genomes database demonstrated that genes in the ceRNA network were primarily involved in the development and adverse outcomes of GDM, such as those in the fatty acid-metabolism pathway, the peroxisome proliferator-activated receptor signaling pathway, and thyroid hormone signaling pathway. Four m6A-related lncRNAs were involved in the ceRNA network, including LINC00667, LINC01087, AP000350.6, and CARMN. The m6A-related subnetwork was generated based on these four lncRNAs, their ceRNAs, and their related m6A regulators. Genes in the subnetwork were enriched in certain GDM-associated hormone (thyroid hormone and oxytocin) signaling pathways. LINC00667 was positively correlated with an m6A “reader” (YTHDF3; PCC = 0.95) and exhibited the highest node degree in the ceRNA network. RIP assays showed that YTHDF3 directly bind LINC00667. We further found that MYC possessed the highest node degree in a protein–protein interaction network and competed with LINC00667 for miR-33a-5p. qPCR analysis indicated that LINC00667, YTHDF3 and MYC levels were upregulated in the GDM placentas, while miR-33a-5p was downregulated. In a support-vector machine classifier, an m6A-related module composed of LINC00667, YTHDF3, MYC, and miR-33a-5p showed excellent classifying power for GDM in both the training and the testing dataset, with an accuracy of 76.19 and 71.43%, respectively.

Conclusions

Our results shed insights into the potential role of m6A-related lncRNAs in GDM and have implications in terms of novel therapeutic targets for GDM.