bluuebunny/crossref_metadata_2025 huggingface dataset:
The Contested Appropriation of George Gervase's Martyrdom: European Religious Patronage and the Controversy over the Oath of Allegiance
Authors: Kelly James E.
Published: March 2018

From the beginning of the seventeenth century, Englishmen professed as Benedictine monks in mainland Europe began returning to their homeland. Until that point, the Catholic mission to England had been run by secular clergy and Jesuits, relationships between the two clerical parties growing increasingly troubled over how the Catholic Reformation should be implemented in England. The arrival of the Benedictines saw the offering of a “third way” to England's proscribed Catholics. Yet with the various missions dependent on lay Catholic resources and support both in England and in mainland Europe, it was necessary for the Benedictines to justify their presence in this often fraught environment. As such, they forcefully laid claim to contemporary English Benedictine martyrs against rival claims by other clerical groups. These battles for validation reached a new level of intensity following James I's serving of the Oath of Allegiance. This article explores how competing groups of English missionary clergy sought to justify their presence in England. Taking the case of two conflicting images of the executed George Gervase, it argues that the contest for martyrs sheds new light on the ways in which martyrdom was exploited by different groups; it also contributes to debates about the Oath of Allegiance, which was threatening to derail the wider Catholic Reformation across mainland Europe. By placing these clashes over English religious identity in both domestic and international contexts, the article makes evident that events on the peripheries of mainland Europe affected discussions at its center.
An NSF Geosciences Road Map to Be Revised with Community Input
Authors: Showstack Randy
Published: March 2018

The agency requests comments by 15 April.
On-chip temporal focusing of elastic waves in a phononic crystal waveguide
Authors: Kurosu M. , Hatanaka D. , Onomitsu K. , Yamaguchi H.
Published: March 2018

The ability to manipulate acoustic and elastic waveforms in continuous media has attracted significant research interest and is crucial for practical applications ranging from biological imaging to material characterization. Although several spatial focusing techniques have been developed, these systems require sophisticated resonant structures with narrow bandwidth, which limit their practical applications. Here we demonstrate temporal pulse manipulation in a dispersive one-dimensional phononic crystal waveguide, which enables the temporal control of ultrasonic wave propagation. On-chip pulse focusing is realized at a desired time and position with chirped input pulses that agree perfectly with the theoretical prediction. Moreover, traveling four-wave mixing experiments are implemented, providing a platform on which to realize novel nonlinear phenomena in the system. Incorporating this dispersive pulse engineering scheme into nonlinear phononic crystal architecture opens up the possibility of investigating novel phenomena such as phononic solitons.
Origin of large plasticity and multiscale effects in iron-based metallic glasses
Authors: Sarac Baran , Ivanov Yurii P. , Chuvilin Andrey , Schöberl Thomas , Stoica Mihai , Zhang Zaoli , Eckert Jürgen
Published: March 2018

The large plasticity observed in newly developed monolithic bulk metallic glasses under quasi-static compression raises a question about the contribution of atomic scale effects. Here, nanocrystals on the order of 1–1.5 nm in size are observed within an Fe-based bulk metallic glass using aberration-corrected high-resolution transmission electron microscopy (HRTEM). The accumulation of nanocrystals is linked to the presence of hard and soft zones, which is connected to the micro-scale hardness and elastic modulus confirmed by nanoindentation. Furthermore, we performed systematic simulations of HRTEM images at varying sample thicknesses, and established a theoretical model for the estimation of the shear transformation zone size. The findings suggest that the main mechanism behind the formation of softer regions are the homogenously dispersed nanocrystals, which are responsible for the start and stop mechanism of shear transformation zones and hence, play a key role in the enhancement of mechanical properties.
Control of laser plasma accelerated electrons for light sources
Authors: André T. , Andriyash I. A. , Loulergue A. , Labat M. , Roussel E. , Ghaith A. , Khojoyan M. , Thaury C. , Valléau M. , Briquez F. , Marteau F. , Tavakoli K. , N’Gotta P. , Dietrich Y. , Lambert G. , Malka V. , Benabderrahmane C. , Vétéran J. , Chapuis L. , El Ajjouri T. , Sebdaoui M. , Hubert N. , Marcouillé O. , Berteaud P. , Leclercq N. , El Ajjouri M. , Rommeluère P. , Bouvet F. , Duval J. -P. , Kitegi C. , Blache F. , Mahieu B. , Corde S. , Gautier J. , Ta Phuoc K. , Goddet J. P. , Lestrade A. , Herbeaux C. , Évain C. , Szwaj C. , Bielawski S. , Tafzi A. , Rousseau P. , Smartsev S. , Polack F. , Dennetière D. , Bourassin-Bouchet C. , De Oliveira C. , Couprie M.-E.
Published: March 2018

With gigaelectron-volts per centimetre energy gains and femtosecond electron beams, laser wakefield acceleration (LWFA) is a promising candidate for applications, such as ultrafast electron diffraction, multistaged colliders and radiation sources (betatron, compton, undulator, free electron laser). However, for some of these applications, the beam performance, for example, energy spread, divergence and shot-to-shot fluctuations, need a drastic improvement. Here, we show that, using a dedicated transport line, we can mitigate these initial weaknesses. We demonstrate that we can manipulate the beam longitudinal and transverse phase-space of the presently available LWFA beams. Indeed, we separately correct orbit mis-steerings and minimise dispersion thanks to specially designed variable strength quadrupoles, and select the useful energy range passing through a slit in a magnetic chicane. Therefore, this matched electron beam leads to the successful observation of undulator synchrotron radiation after an 8 m transport path. These results pave the way to applications demanding in terms of beam quality.
Recurrent lower respiratory illnesses among young children in rural Kyrgyzstan: overuse of antibiotics and possible under-diagnosis of asthma. A qualitative FRESH AIR study
Authors: Østergaard Marianne Stubbe , Kjærgaard Jesper , Kristensen Mette Marie , Reventlow Susanne , Poulsen Anja , Isaeva Elvira , Akylbekov Azamat , Sooronbaev Talant
Published: March 2018

Lower respiratory tract illnesses (LRT-illnesses) in children under 5 years (U5s) are a leading cause of morbidity, hospitalisations and mortality worldwide, particularly in low-income countries. It is pertinent to understand possible inconsistent management. This study explored perceptions and practices among caregivers and health professionals on recurrent LRT-illnesses in U5s. Semi-structured interviews with 13 caregivers to U5s with recurrent LRT-illnesses and with 22 primary care health professional interviews in two rural provinces in Kyrgyzstan were triangulated. Data were thematically analysed. The majority (8/13) of caregivers described their young children as having recurrent coughing, noisy breathing and respiratory distress of whom several had responded positively to acute salbutamol and/or had been repeatedly hospitalised for LRT-illness. Family stress and financial burdens were significant. The health professionals classified young children with recurrent LRT-illnesses primarily with pneumonia and/or a multitude of bronchitis diagnoses. Broad-spectrum antibiotics and supportive medicine were used repeatedly, prescribed by health professionals or purchased un-prescribed by the caregivers at the pharmacy. The health professionals had never applied the asthma diagnosis to U5s nor had they prescribed inhaled steroids, and none of the interviewed caregivers’ U5s were diagnosed with asthma. Health professionals and caregivers shared a common concern for the children’s recurrent respiratory illnesses developing into a severe chronic pulmonary condition, including asthma. In conclusion, the study identified an inconsistent management of LRT-illnesses in U5s, with exorbitant use of antibiotics and an apparently systemic under-diagnosis of asthma/wheeze. When the diagnosis asthma is not used, the illness is not considered as a long-term condition, requiring preventer/controller medication.
Hydrogel-based scaffolds to support intrathecal stem cell transplantation as a gateway to the spinal cord: clinical needs, biomaterials, and imaging technologies
Authors: Oliveira J. Miguel , Carvalho Luisa , Silva-Correia Joana , Vieira Sílvia , Majchrzak Malgorzata , Lukomska Barbara , Stanaszek Luiza , Strymecka Paulina , Malysz-Cymborska Izabela , Golubczyk Dominika , Kalkowski Lukasz , Reis Rui L. , Janowski Miroslaw , Walczak Piotr
Published: March 2018

The prospects for cell replacement in spinal cord diseases are impeded by inefficient stem cell delivery. The deep location of the spinal cord and complex surgical access, as well as densely packed vital structures, question the feasibility of the widespread use of multiple spinal cord punctures to inject stem cells. Disorders characterized by disseminated pathology are particularly appealing for the distribution of cells globally throughout the spinal cord in a minimally invasive fashion. The intrathecal space, with access to a relatively large surface area along the spinal cord, is an attractive route for global stem cell delivery, and, indeed, is highly promising, but the success of this approach relies on the ability of cells (1) to survive in the cerebrospinal fluid (CSF), (2) to adhere to the spinal cord surface, and (3) to migrate, ultimately, into the parenchyma. Intrathecal infusion of cell suspension, however, has been insufficient and we postulate that embedding transplanted cells within hydrogel scaffolds will facilitate reaching these goals. In this review, we focus on practical considerations that render the intrathecal approach clinically viable, and then discuss the characteristics of various biomaterials that are suitable to serve as scaffolds. We also propose strategies to modulate the local microenvironment with nanoparticle carriers to improve the functionality of cellular grafts. Finally, we provide an overview of imaging modalities for in vivo monitoring and characterization of biomaterials and stem cells. This comprehensive review should serve as a guide for those planning preclinical and clinical studies on intrathecal stem cell transplantation.
A systems pharmacology-oriented discovery of a new therapeutic use of the TCM formula Liuweiwuling for liver failure
Authors: Wang Jia-bo , Cui He-rong , Wang Rui-lin , Zhang Cong-en , Niu Ming , Bai Zhao-fang , Xu Gen-hua , Li Peng-yan , Jiang Wen-yan , Han Jing-jing , Ma Xiao , Cai Guang-ming , Li Rui-sheng , Zhang Li-ping , Xiao Xiao-he
Published: March 2018

Multiple components of traditional Chinese medicine (TCM) formulae determine their treatment targets for multiple diseases as opposed to a particular disease. However, discovering the unexplored therapeutic potential of a TCM formula remains challenging and costly. Inspired by the drug repositioning methodology, we propose an integrated strategy to feasibly identify new therapeutic uses for a formula composed of six herbs, Liuweiwuling. First, we developed a comprehensive systems approach to enrich drug compound-liver disease networks to analyse the major predicted diseases of Liuweiwuling and discover its potential effect on liver failure. The underlying mechanisms were subsequently predicted to mainly attribute to a blockade of hepatocyte apoptosis via a synergistic combination of multiple effects. Next, a classical pharmacology experiment was designed to validate the effects of Liuweiwuling on different models of fulminant liver failure induced by D-galactosamine/lipopolysaccharide (GalN/LPS) or thioacetamide (TAA). The results indicated that pretreatment with Liuweiwuling restored liver function and reduced lethality induced by GalN/LPS or TAA in a dose-dependent manner, which was partially attributable to the abrogation of hepatocyte apoptosis by multiple synergistic effects. In summary, the integrated strategy discussed in this paper may provide a new approach for the more efficient discovery of new therapeutic uses for TCM formulae.
Intentional communication between wild bonnet macaques and humans
Authors: Deshpande Adwait , Gupta Shreejata , Sinha Anindya
Published: March 2018

Comparative studies of nonhuman communication systems could provide insights into the origins and evolution of a distinct dimension of human language: intentionality. Recent studies have provided evidence for intentional communication in different species but generally in captive settings. We report here a novel behaviour of food requesting from humans displayed by wild bonnet macaques Macaca radiata, an Old World cercopithecine primate, in the Bandipur National Park of southern India. Using both natural observations and field experiments, we examined four different behavioural components—coo-calls, hand-extension gesture, orientation, and monitoring behaviour—of food requesting for their conformity with the established criteria of intentional communication. Our results suggest that food requesting by bonnet macaques is potentially an intentionally produced behavioural strategy as all the food requesting behaviours except coo-calls qualify the criteria for intentionality. We comment on plausible hypotheses for the origin and spread of this novel behavioural strategy in the study macaque population and speculate that the cognitive precursors for language production may be manifest in the usage of combination of signals of different modalities in communication, which could have emerged in simians earlier than in the anthropoid apes.
Effects of high pressure on the electrical resistivity and dielectric properties of nanocrystalline SnO
2
Authors: Shen Wenshu , Ou Tianji , Wang Jia , Qin Tianru , Zhang Guozhao , Zhang Xin , Han Yonghao , Ma Yanzhang , Gao Chunxiao
Published: March 2018

The electrical transport and structural properties of tin oxide nanoparticles under compression have been studied by in situ impedance measurements and synchrotron X-ray diffraction (XRD) up to 27.9 GPa. It was found that the conduction of SnO2 can be improved significantly with compression. Abnormal variations in resistivity, relaxation frequency, and relative permittivity were observed at approximately 12.3 and 25.0 GPa, which can be attributed to pressure-induced tetragonal- orthorhombic-cubic structural transitions. The dielectric properties of the SnO2 nanoparticles were found to be a function of pressure, and the dielectric response was dependent on frequency and pressure. The dielectric constant and loss tangent decreased with increasing frequency. Relaxation-type dielectric behaviour dominated at low frequencies. Whereas, modulus spectra indicated that charge carrier short-range motion dominated at high frequencies.
Diffuse Myocardial Injuries are Present in Subclinical Hypothyroidism: A Clinical Study Using Myocardial T1-mapping Quantification
Authors: Yao Zhi , Gao Xia , Liu Min , Chen Zhe , Yang Ning , Jia Yu-Mei , Feng Xiao-Meng , Xu Yuan , Yang Xin-Chun , Wang Guang
Published: March 2018

Subclinical hypothyroidism (SHT) is a common disorder that may represent early thyroid dysfunction and is related to adverse cardiovascular events. However, myocardial injuries induced by SHT are difficult to detect. Our previous study demonstrated that the cardiac magnetic resonance (CMR) myocardial longitudinal relaxation time (T1) mapping technique is a useful tool for assessing diffuse myocardial injuries in overt hypothyroidism patients. This study was designed to detect whether diffuse myocardial injuries were present in SHT by using the T1 mapping technique. We found that SHT participants had significantly increased native T1 values within four segments of the left ventricle (all p < 0.01), especially patients with thyroid-stimulating hormone (TSH) levels ≥10 µIU/mL, compared with those in the controls. In addition, the native T1 values were negatively correlated with free thyroxine (FT4) (r = −0.476, p = 0.003) and were positively correlated with TSH (r = 0.489, p = 0.002). Furthermore, left ventricular diastolic function estimated by the peak filling rate (PFR) was significantly lower in patients with TSH levels ≥10 µIU/mL than that in the controls (p < 0.05). In conclusion, diffuse myocardial injuries were present in SHT, and T1 mapping may be a useful tool for evaluating mild myocardial injuries in SHT at an early stage. Our study is the first to confirm myocardial injuries in SHT patients using T1 mapping.
Crotamine induces browning of adipose tissue and increases energy expenditure in mice
Authors: Marinovic Marcelo P. , Campeiro Joana D. , Lima Sunamita C. , Rocha Andrea L. , Nering Marcela B. , Oliveira Eduardo B. , Mori Marcelo A. , Hayashi Mirian A. F.
Published: March 2018

Crotamine, originally isolated from rattlesnake venom, has been extensively studied due to its pleiotropic biological properties, and special attention has been paid to its antitumor activity. However, long-term treatment with crotamine was accompanied by a reduction in animal body weight gain and by increases in glucose tolerance. As cancer is commonly associated with cachexia, to preclude the possible cancer cachexia-like effect of crotamine, herein this polypeptide was administered in healthy wild-type C57/BL6 mice by the oral route daily, for 21 days. Reduced body weight gain, in addition to decreased white adipose tissue (WAT) and increased brown adipose tissue (BAT) mass were observed in healthy animals in the absence of tumor. In addition, we observed improved glucose tolerance and increased insulin sensitivity, accompanied by a reduction of plasma lipid levels and decreased levels of biomarkers of liver damage and kidney disfunctions. Importantly, long-term treatment with crotamine increased the basal metabolic ratein vivo, which was consistent with the increased expression of thermogenic markers in BAT and WAT. Interestingly, cultured brown adipocyte cells induced to differentiation in the presence of crotamine also showed increases in some of these markers and in lipid droplets number and size, indicating increased brown adipocyte maturation.
LFP beta amplitude is linked to mesoscopic spatio-temporal phase patterns
Authors: Denker Michael , Zehl Lyuba , Kilavik Bjørg E. , Diesmann Markus , Brochier Thomas , Riehle Alexa , Grün Sonja
Published: March 2018

Beta oscillations observed in motor cortical local field potentials (LFPs) recorded on separate electrodes of a multi-electrode array have been shown to exhibit non-zero phase shifts that organize into planar waves. Here, we generalize this concept to additional classes of salient patterns that fully describe the spatial organization of beta oscillations. During a delayed reach-to-grasp task we distinguish planar, synchronized, random, circular, and radial phase patterns in monkey primary motor and dorsal premotor cortices. We observe that patterns correlate with the beta amplitude (envelope): Coherent planar/radial wave propagation accelerates with growing amplitude, and synchronized patterns are observed at largest amplitudes. In contrast, incoherent random or circular patterns are observed almost exclusively when beta is strongly attenuated. The occurrence probability of a particular pattern modulates with behavioral epochs in the same way as beta amplitude: Coherent patterns are more present during movement preparation where amplitudes are large, while incoherent phase patterns are dominant during movement execution where amplitudes are small. Thus, we uncover a trigonal link between the spatial arrangement of beta phases, beta amplitude, and behavior. Together with previous findings, we discuss predictions on the spatio-temporal organization of precisely coordinated spiking on the mesoscopic scale as a function of beta power.
Urban and nomadic isotopic niches reveal dietary connectivities along Central Asia’s Silk Roads
Authors: Hermes Taylor R. , Frachetti Michael D. , Bullion Elissa A. , Maksudov Farhod , Mustafokulov Samariddin , Makarewicz Cheryl A.
Published: March 2018

The ancient ‘Silk Roads’ formed a vast network of trade and exchange that facilitated the movement of commodities and agricultural products across medieval Central Asia via settled urban communities and mobile pastoralists. Considering food consumption patterns as an expression of socio-economic interaction, we analyse human remains for carbon and nitrogen isotopes in order to establish dietary intake, then model isotopic niches to characterize dietary diversity and infer connectivity among communities of urbanites and nomadic pastoralists. The combination of low isotopic variation visible within urban groups with isotopic distinction between urban communities irrespective of local environmental conditions strongly suggests localized food production systems provided primary subsistence rather than agricultural goods exchanged along trade routes. Nomadic communities, in contrast, experienced higher dietary diversity reflecting engagements with a wide assortment of foodstuffs typical for mobile communities. These data indicate tightly bound social connectivity in urban centres pointedly funnelled local food products and homogenized dietary intake within settled communities, whereas open and opportunistic systems of food production and circulation were possible through more mobile lifeways.
Association between serum uric acid and cardiovascular risk in nonhypertensive and nondiabetic individuals: The Taiwan I-Lan Longitudinal Aging Study
Authors: Chang Chun-Chin , Wu Cheng-Hsueh , Liu Li-Kuo , Chou Ruey-Hsing , Kuo Chin-Sung , Huang Po-Hsun , Chen Liang-Kung , Lin Shing-Jong
Published: March 2018

Serum uric acid level is a risk factor for cardiovascular disease (CVD). However, whether it is an independent risk factor or not remains controversial. We analyzed the association between serum uric acid level and cardiovascular risk. In total, 973 nonhypertensive and nondiabetic participants in the I-Lan Longitudinal Aging Study were eligible for this study. Subjects were divided into tertiles according to uric acid levels. The 10-year cardiovascular risk was calculated using Framingham risk score (FRS). Study subjects in the highest tertile of serum uric acid level were older, more likely to be male, and had higher systolic blood pressure, body mass index, carotid artery intima–media thickness and serum triglyceride, high-sensitivity C-reactive protein, and low-density lipoprotein cholesterol levels and lower serum high-density lipoprotein cholesterol levels (all p < 0.05). Subjects in the highest tertile had significantly higher FRS (p < 0.001). After adjusting for other risk factors, serum uric acid level remained associated significantly with the FRS (p < 0.05). In binary logistic regression analysis, the serum uric acid level was an independent predictive factor for high (≥20%) FRS (odds ratio 1.33, 95% confidence interval 1.10–1.68). These findings warrant attention to this cardiovascular risk factor in apparently healthy adults.
Bi-directional coupling in strain-mediated multiferroic heterostructures with magnetic domains and domain wall motion
Authors: Xiao Zhuyun , Lo Conte Roberto , Chen Cai , Liang Cheng-Yen , Sepulveda Abdon , Bokor Jeffrey , Carman Gregory P. , Candler Robert N.
Published: March 2018

Strain-coupled multiferroic heterostructures provide a path to energy-efficient, voltage-controlled magnetic nanoscale devices, a region where current-based methods of magnetic control suffer from Ohmic dissipation. Growing interest in highly magnetoelastic materials, such as Terfenol-D, prompts a more accurate understanding of their magnetization behavior. To address this need, we simulate the strain-induced magnetization change with two modeling methods: the commonly used unidirectional model and the recently developed bidirectional model. Unidirectional models account for magnetoelastic effects only, while bidirectional models account for both magnetoelastic and magnetostrictive effects. We found unidirectional models are on par with bidirectional models when describing the magnetic behavior in weakly magnetoelastic materials (e.g., Nickel), but the two models deviate when highly magnetoelastic materials (e.g., Terfenol-D) are introduced. These results suggest that magnetostrictive feedback is critical for modeling highly magnetoelastic materials, as opposed to weaker magnetoelastic materials, where we observe only minor differences between the two methods’ outputs. To our best knowledge, this work represents the first comparison of unidirectional and bidirectional modeling in composite multiferroic systems, demonstrating that back-coupling of magnetization to strain can inhibit formation and rotation of magnetic states, highlighting the need to revisit the assumption that unidirectional modeling always captures the necessary physics in strain-mediated multiferroics.
Sulfate alters aerosol absorption properties in East Asian outflow
Authors: Lim Saehee , Lee Meehye , Kim Sang-Woo , Laj Paolo
Published: March 2018

Black carbon (BC) and brown carbon (BrC) aerosols that are released from the combustion of fossil fuels and biomass are of great concern because of their light-absorbing ability and great abundance associated with various anthropogenic sources, particularly in East Asia. However, the optical properties of ambient aerosols are dependent on the mixing state and the chemical composition of absorbing and non-absorbing aerosols. Here we examined how, in East Asian outflows, the parameters of the aerosol optical properties can be altered seasonally in conjunction with the mixing state and the chemical composition of aerosols, using 3-year aerosol measurements. Our findings highlight the important role played by sulfate in East Asia during the warm season in both enhancing single scattering albedo (SSA) and altering the absorption properties of aerosols—enhancing mass absorption cross section of BC (MACBC) and reducing MAC of BrC (MACBrC,370). Therefore we suggest that in global radiative forcing models, particular attention should be paid to the consideration of the accurate treatment of the SO2 emission changes in the coming years in this region that will result from China’s air quality policy.
Author Correction: Vitamin E inhibits the UVAI induction of “light” and “dark” cyclobutane pyrimidine dimers, and oxidatively generated DNA damage, in keratinocytes
Authors: Delinasios George J. , Karbaschi Mahsa , Cooke Marcus S. , Young Antony R.
Published: March 2018

A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper.
Author Correction: Improvement of Pharmacokinetic Profile of TRAIL via Trimer-Tag Enhances its Antitumor Activity in vivo
Authors: Liu Haipeng , Su Danmei , Zhang Jinlong , Ge Shuaishuai , Li Youwei , Wang Fei , Gravel Michel , Roulston Anne , Song Qin , Xu Wei , Liang Joshua G. , Shore Gordon , Wang Xiaodong , Liang Peng
Published: March 2018

A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper.
Heparanase attenuates axon degeneration following sciatic nerve transection
Authors: Whitehead Michael J. , McGonigal Rhona , Willison Hugh J. , Barnett Susan C.
Published: March 2018

Axon degeneration underlies many nervous system diseases; therefore understanding the regulatory signalling pathways is fundamental to identifying potential therapeutics. Previously, we demonstrated heparan sulphates (HS) as a potentially new target for promoting CNS repair. HS modulate cell signalling by both acting as cofactors in the formation of ligand-receptor complexes and in sequestering ligands in the extracellular matrix. The enzyme heparanase (Hpse) negatively regulates these processes by cleaving HS and releasing the attached proteins, thereby attenuating their ligand-receptor interaction. To explore a comparative role for HS in PNS axon injury/repair we data mined published microarrays from distal sciatic nerve injury. We identified Hpse as a previously unexplored candidate, being up-regulated following injury. We confirmed these results and demonstrated inhibition of Hpse led to an acceleration of axonal degeneration, accompanied by an increase in β-catenin. Inhibition of β-catenin and the addition of Heparinase I both attenuated axonal degeneration. Furthermore the inhibition of Hpse positively regulates transcription of genes associated with peripheral neuropathies and Schwann cell de-differentiation. Thus, we propose Hpse participates in the regulation of the Schwann cell injury response and axo-glia support, in part via the regulation of Schwann cell de-differentiation and is a potential therapeutic that warrants further investigation.
Floral resource partitioning by individuals within generalised hoverfly pollination networks revealed by DNA metabarcoding
Authors: Lucas Andrew , Bodger Owen , Brosi Berry J. , Ford Col R. , Forman Dan W. , Greig Carolyn , Hegarty Matthew , Jones Laura , Neyland Penelope J. , de Vere Natasha
Published: March 2018

Pollination is a key ecosystem service for agriculture and wider ecosystem function. However, most pollination studies focus on Hymenoptera, with hoverflies (Syrphidae) frequently treated as a single functional group. We tested this assumption by investigating pollen carried by eleven species of hoverfly in five genera, Cheilosia, Eristalis, Rhingia, Sericomyia and Volucella, using DNA metabarcoding. Hoverflies carried pollen from 59 plant taxa, suggesting they visit a wider number of plant species than previously appreciated. Most pollen recorded came from plant taxa frequently found at our study sites, predominantly Apiaceae, Cardueae, Calluna vulgaris, Rubus fruticosus agg., and Succisa pratensis, with hoverflies transporting pollen from 40% of entomophilous plant species present. Overall pollen transport network structures were generalised, similar to other pollination networks elsewhere. All hoverfly species were also generalised with few exclusive plant/hoverfly interactions. However, using the Jaccard Index, we found significant differences in the relative composition of pollen loads between hoverfly genera, except for Volucella, demonstrating some degree of functional complementarity. Eristalis and Sericomyia species had significant differences in relative pollen load composition compared to congeners. Our results demonstrate the range of pollens transported by hoverflies and the potential pollination function undertaken within this ecologically and morphologically diverse guild.
Same habitat types but different use: evidence of context-dependent habitat selection in roe deer across populations
Authors: William Gaudry , Jean-Michel Gaillard , Sonia Saïd , Christophe Bonenfant , Atle Mysterud , Nicolas Morellet , Maryline Pellerin , Clément Calenge
Published: March 2018

With the surge of GPS-technology, many studies uncovered space use of mobile animals and shed light on the underlying behavioral mechanisms of habitat selection. Habitat selection and variation in either occurrence or strength of functional responses (i.e. how selection changes with availability) have given new insight into such mechanisms within populations in different ecosystems. However, linking variation in habitat selection to site-specific conditions in different populations facing contrasting environmental conditions but the same habitat type has not yet been investigated. We aimed to fill this knowledge gap by comparing within-home range habitat selection across 61 female roe deer (Capreolus capreolus) during the most critical life history stage in three study areas showing the same habitat types but with different environmental conditions. Female roe deer markedly differed in habitat selection within their home range, both within and among populations. Females facing poor environmental conditions clearly displayed a functional response, whereas females facing rich environmental conditions did not show any functional response. These results demonstrate how the use of a given habitat relative to its availability strongly varies in response to environmental conditions. Our findings highlight that the same habitat composition can lead to very different habitat selection processes across contrasted environments.
Laser speckle flowgraphy derived characteristics of optic nerve head perfusion in normal tension glaucoma and healthy individuals: a Pilot study
Authors: Mursch-Edlmayr Anna Sophie , Luft Nikolaus , Podkowinski Dominika , Ring Michael , Schmetterer Leopold , Bolz Matthias
Published: March 2018

The purpose of this prospective, case control study was to investigate the differences in optic nerve head blood flow measured with Laser Speckle Flowgraphy (LSFG) between Caucasian patients with normal tension glaucoma and healthy subjects. It included 20 eyes from 20 Caucasian patients with diagnosis of normal tension glaucoma and 20 eyes from age- and sex-matched healthy individuals. In the glaucoma group the antiglaucomatous therapy was paused 3 weeks prior to the investigations. Measurement of optic nerve head blood flow was performed with LSFG. The mean blur rate was obtained for different vascular compartments of the optic nerve head. Parameters for the characterization of pulse-waveform of the mean blur rate were calculated. It was shown that the mean blur rate was significantly lower in the glaucoma group compared to the control group (P < 0.001). The significant differences in the pulse-waveform parameters blow out time (P = 0.028) and flow acceleration time index (P < 0.001) indicate a flatter curve in NTG patients. In conclusion, LSFG can detect differences in optic nerve head blood flow between eyes with normal tension glaucoma and healthy eyes.
Author Correction: Modulation of van der Waals and classical epitaxy induced by strain at the Si step edges in GeSbTe alloys
Authors: Zallo Eugenio , Cecchi Stefano , Boschker Jos E. , Mio Antonio M. , Arciprete Fabrizio , Privitera Stefania , Calarco Raffaella
Published: March 2018

A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper.
Intriguingly high thermal conductivity increment for CuO nanowires contained nanofluids with low viscosity
Authors: Zhu Dahai , Wang Lingling , Yu Wei , Xie Huaqing
Published: March 2018

Nanofluids offer the exciting new possibilities to enhance heat transfer performance. In this paper, experimental and theoretical investigations have been conducted to determine the effect of CuO nanowires on the thermal conductivity and viscosity of dimethicone based nanofluids. The CuO nanowires were prepared through a thermal oxidation method, and the analysis indicated that the as-prepared CuO nanowires had high purity, monocrystalline with a monoclinic structure and large aspect ratio compared to CuO nanospheres. The experimental data show that the thermal conductivity of the nanofluids increases with the volume fraction of CuO nanowires or nanospheres, with a nearly linear relationship. For the nanofluid with the addition of 0.75 vol.% CuO nanowires, the thermal conductivity enhancement is up to 60.78%, which is much higher than that with spherical CuO nanoparticles. The nanofluids exhibit typical Newtonian behavior, and the measured viscosity of CuO nanowires contained nanofluids were found only 6.41% increment at the volume fraction of 0.75%. It is attractive in enhanced heat transfer for application. The thermal conductivity and viscosity of CuO nanofluids were further calculated and discussed by comparing our experimental results with the classic theoretical models. The mechanisms of thermal conductivity and viscosity about nanofluids were also discussed in detail.
Iron regulatory protein deficiency compromises mitochondrial function in murine embryonic fibroblasts
Authors: Li Huihui , Zhao Hongting , Hao Shuangying , Shang Longcheng , Wu Jing , Song Chuanhui , Meyron-Holtz Esther G. , Qiao Tong , Li Kuanyu
Published: March 2018

Iron is essential for growth and proliferation of mammalian cells. The maintenance of cellular iron homeostasis is regulated by iron regulatory proteins (IRPs) through binding to the cognate iron-responsive elements in target mRNAs and thereby regulating the expression of target genes. Irp1 or Irp2-null mutation is known to reduce the cellular iron level by decreasing transferrin receptor 1 and increasing ferritin. Here, we report that Irp1 or Irp2-null mutation also causes downregulation of frataxin and IscU, two of the core components in the iron-sulfur cluster biogenesis machinery. Interestingly, while the activities of some of iron-sulfur cluster-containing enzymes including mitochondrial aconitase and cytosolic xanthine oxidase were not affected by the mutations, the activities of respiratory chain complexes were drastically diminished resulting in mitochondrial dysfunction. Overexpression of human ISCU and frataxin in Irp1 or Irp2-null cells was able to rescue the defects in iron-sulfur cluster biogenesis and mitochondrial quality. Our results strongly suggest that iron regulatory proteins regulate the part of iron sulfur cluster biogenesis tailored specifically for mitochondrial electron transport chain complexes.
Author Correction: UClncR: Ultrafast and comprehensive long non-coding RNA detection from RNA-seq
Authors: Sun Zhifu , Nair Asha , Chen Xianfeng , Prodduturi Naresh , Wang Junwen , Kocher Jean-Pierre
Published: March 2018

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Ras hyperactivation versus overexpression: Lessons from Ras dynamics in Candida albicans
Authors: Pratyusha Vavilala A. , Victoria Guiliana Soraya , Khan Mohammad Firoz , Haokip Dominic T. , Yadav Bhawna , Pal Nibedita , Sethi Subhash Chandra , Jain Priyanka , Singh Sneh Lata , Sen Sobhan , Komath Sneha Sudha
Published: March 2018

Ras signaling in response to environmental cues is critical for cellular morphogenesis in eukaryotes. This signaling is tightly regulated and its activation involves multiple players. Sometimes Ras signaling may be hyperactivated. In C. albicans, a human pathogenic fungus, we demonstrate that dynamics of hyperactivated Ras1 (Ras1G13V or Ras1 in Hsp90 deficient strains) can be reliably differentiated from that of normal Ras1 at (near) single molecule level using fluorescence correlation spectroscopy (FCS). Ras1 hyperactivation results in significantly slower dynamics due to actin polymerization. Activating actin polymerization by jasplakinolide can produce hyperactivated Ras1 dynamics. In a sterol-deficient hyperfilamentous GPI mutant of C. albicans too, Ras1 hyperactivation results from Hsp90 downregulation and causes actin polymerization. Hyperactivated Ras1 co-localizes with G-actin at the plasma membrane rather than with F-actin. Depolymerizing actin with cytochalasin D results in faster Ras1 dynamics in these and other strains that show Ras1 hyperactivation. Further, ergosterol does not influence Ras1 dynamics.
The role of cuticular hydrocarbons in mate recognition in Drosophila suzukii
Authors: Snellings Yannick , Herrera Beatriz , Wildemann Bruna , Beelen Melissa , Zwarts Liesbeth , Wenseleers Tom , Callaerts Patrick
Published: March 2018

Cuticular hydrocarbons (CHCs) play a central role in the chemical communication of many insects. In Drosophila suzukii, an economically important pest insect, very little is known about chemical communication and the possible role of CHCs. In this study, we identified 60 CHCs of Drosophila suzukii and studied their changes in function of age (maturation), sex and interactions with the opposite sex. We demonstrate that age (maturation) is the key factor driving changes in the CHC profiles. We then test the effect on courtship behaviour and mating of six CHCs, five of which were positively associated with maturation and one negatively. The results of these experiments demonstrate that four of the major CHC peaks with a chain length of 23 carbons, namely 9-tricosene (9-C23:1), 7-tricosene (7-C23:1), 5-tricosene (5-C23:1) and tricosane (n-C23), negatively regulated courtship and mating, even though all these compounds were characteristic for sexually mature flies. We then go on to show that this effect on courtship and mating is likely due to the disruption of the natural ratios in which these hydrocarbons occur in Drosophila suzukii. Overall, these results provide key insights into the cuticular hydrocarbon signals that play a role in D. suzukii mate recognition.
Effects of Structural and Energetic Disorders on Charge Transports in Crystal and Amorphous Organic Layers
Authors: Suzuki Furitsu , Kubo Shosei , Fukushima Tatsuya , Kaji Hironori
Published: March 2018

Understanding charge transports in organic films is important for both fundamental science and practical applications. Here, contributions of off-diagonal (structural) and diagonal (energetic) disorders to charge transports were clarified using molecular-based multiscale simulation. These disorders, important for understanding charge transport in organic systems, are investigated by comparing crystal and amorphous aggregates of N,N′-diphenyl-N,N′-bis(1-naphthyl)-1,1′-biphenyl-4,4′-diamine (NPD). Although NPD has been used as a hole transport material, it also exhibits comparable electron mobility experimentally. The experimental mobility and its electric field dependence in amorphous layers were reasonably reproduced by the multiscale simulation, confirming the electron transport properties of NPD. We assumed that the structural disorder would lower mobilities; however, the mobilities were found to be independent of the degree of structural disorder. Energetic disorder markedly lowered charge mobility instead. Charge migration in crystals was dominated by maximum electronic coupling pairs, whereas small electronic coupling pairs significantly contributed to charge transport in amorphous aggregate.
SpliceDetector: a software for detection of alternative splicing events in human and model organisms directly from transcript IDs
Authors: Baharlou Houreh Mandana , Ghorbani Kalkhajeh Payam , Niazi Ali , Ebrahimi Faezeh , Ebrahimie Esmaeil
Published: March 2018

In eukaryotes, different combinations of exons lead to multiple transcripts with various functions in protein level, in a process called alternative splicing (AS). Unfolding the complexity of functional genomics through genome-wide profiling of AS and determining the altered ultimate products provide new insights for better understanding of many biological processes, disease progress as well as drug development programs to target harmful splicing variants. The current available tools of alternative splicing work with raw data and include heavy computation. In particular, there is a shortcoming in tools to discover AS events directly from transcripts. Here, we developed a Windows-based user-friendly tool for identifying AS events from transcripts without the need to any advanced computer skill or database download. Meanwhile, due to online working mode, our application employs the updated SpliceGraphs without the need to any resource updating. First, SpliceGraph forms based on the frequency of active splice sites in pre-mRNA. Then, the presented approach compares query transcript exons to SpliceGraph exons. The tool provides the possibility of statistical analysis of AS events as well as AS visualization compared to SpliceGraph. The developed application works for transcript sets in human and model organisms.
A micro-CT-based method for quantitative brain lesion characterization and electrode localization
Authors: Masís Javier , Mankus David , Wolff Steffen B. E. , Guitchounts Grigori , Joesch Maximilian , Cox David D.
Published: March 2018

Lesion verification and quantification is traditionally done via histological examination of sectioned brains, a time-consuming process that relies heavily on manual estimation. Such methods are particularly problematic in posterior cortical regions (e.g. visual cortex), where sectioning leads to significant damage and distortion of tissue. Even more challenging is the post hoc localization of micro-electrodes, which relies on the same techniques, suffers from similar drawbacks and requires even higher precision. Here, we propose a new, simple method for quantitative lesion characterization and electrode localization that is less labor-intensive and yields more detailed results than conventional methods. We leverage staining techniques standard in electron microscopy with the use of commodity micro-CT imaging. We stain whole rat and zebra finch brains in osmium tetroxide, embed these in resin and scan entire brains in a micro-CT machine. The scans result in 3D reconstructions of the brains with section thickness dependent on sample size (12–15 and 5–6 microns for rat and zebra finch respectively) that can be segmented manually or automatically. Because the method captures the entire intact brain volume, comparisons within and across studies are more tractable, and the extent of lesions and electrodes may be studied with higher accuracy than with current methods.
A new cell-laden 3D Alginate-Matrigel hydrogel resembles human breast cancer cell malignant morphology, spread and invasion capability observed “in vivo”
Authors: Cavo Marta , Caria Marco , Pulsoni Ilaria , Beltrame Francesco , Fato Marco , Scaglione Silvia
Published: March 2018

Purpose of this study was the development of a 3D material to be used as substrate for breast cancer cell culture. We developed composite gels constituted by different concentrations of Alginate (A) and Matrigel (M) to obtain a structurally stable-in-time and biologically active substrate. Human aggressive breast cancer cells (i.e. MDA-MB-231) were cultured within the gels. Known the link between cell morphology and malignancy, cells were morphologically characterized and their invasiveness correlated through an innovative bioreactor-based invasion assay. A particular type of gel (i.e. 50% Alginate, 50% Matrigel) emerged thanks to a series of significant results: 1. cells exhibited peculiar cytoskeleton shapes and nuclear fragmentation characteristic of their malignancy; 2. cells expressed the formation of the so-called invadopodia, actin-based protrusion of the plasma membrane through which cells anchor to the extracellular matrix; 3. cells were able to migrate through the gels and attach to an engineered membrane mimicking the vascular walls hosted within bioreactor, providing a completely new 3D in vitro model of the very precursor steps of metastasis.
Circatrigintan instead of lunar periodicity of larval release in a brooding coral species
Authors: Linden Bart , Huisman Jef , Rinkevich Baruch
Published: March 2018

Larval release by brooding corals is often assumed to display lunar periodicity. Here, we show that larval release of individual Stylophora pistillata colonies does not comply with the assumed tight entrainment by the lunar cycle, and can better be classified as a circatrigintan pattern. The colonies exhibited three distinct reproductive patterns, characterized by short intervals, long intervals and no periodicity between reproductive peaks, respectively. Cross correlation between the lunar cycle and larval release of the periodic colonies revealed an approximately 30-day periodicity with a variable lag of 5 to 10 days after full moon. The observed variability indicates that the lunar cycle does not provide a strict zeitgeber. Other factors such as water temperature and solar radiation did not correlate significantly with the larval release. The circatrigintan patterns displayed by S. pistillata supports the plasticity of corals and sheds new light on discussions on the fecundity of brooding coral species.