bluuebunny/crossref_metadata_2025 huggingface dataset:
Roles of the α1B-Adrenergic Receptor Phosphorylation Domains in Signaling and Internalization
Authors: Hernández-Espinosa David A. , Alcántara-Hernández Rocío , Solís K. Helivier , García-Sáinz J. Adolfo
Published: November 2023

The function of the α1B-adrenergic receptor phosphorylation sites previously detected by mass spectrometry was evaluated by employing mutants, substituting them with non-phosphorylatable amino acids. Substitution of the intracellular loop 3 (IL3) sites did not alter baseline or stimulated receptor phosphorylation, whereas substitution of phosphorylation sites in the carboxyl terminus (Ctail) or both domains (IL3/Ctail) markedly decreased receptor phosphorylation. Cells expressing the IL3 or Ctail receptor mutants exhibited a noradrenaline-induced calcium-maximal response similar to those expressing the wild-type receptor, and a shift to the left in the concentration–response curve to noradrenaline was also noticed. Cells expressing the IL3/Ctail mutant exhibited higher apparent potency and increased maximal response to noradrenaline than those expressing the wild-type receptor. Phorbol ester-induced desensitization of the calcium response to noradrenaline was reduced in cells expressing the IL3 mutant and abolished in cells in which the Ctail or the IL3/Ctail were modified. In contrast, desensitization in response to preincubation with noradrenaline was unaffected in cells expressing the distinct receptor mutants. Noradrenaline-induced ERK phosphorylation was surprisingly increased in cells expressing IL3-modified receptors but not in those expressing receptors with the Ctail or IL3/Ctail substitutions. Our data indicate that phosphorylation sites in the IL3 and Ctail domains mediate and regulate α1B-adrenergic receptor function. Phorbol ester-induced desensitization seems to be closely associated with receptor phosphorylation, whereas noradrenaline-induced desensitization likely involves other elements.
TBM Hunter: Identify and Score Canonical, Extended, and Unconventional Tankyrase-Binding Motifs in Any Protein
Authors: Clements Christopher M. , Shellman Samantha X. , Shellman Melody H. , Shellman Yiqun G.
Published: November 2023

Tankyrases, a versatile protein group within the poly(ADP-ribose) polymerase family, are essential for post-translational poly(ADP-ribosyl)ation, influencing various cellular functions and contributing to diseases, particularly cancer. Consequently, tankyrases have become important targets for anti-cancer drug development. Emerging approaches in drug discovery aim to disrupt interactions between tankyrases and their binding partners, which hinge on tankyrase-binding motifs (TBMs) within partner proteins and ankyrin repeat cluster domains within tankyrases. Our study addresses the challenge of identifying and ranking TBMs. We have conducted a comprehensive review of the existing literature, classifying TBMs into three distinct groups, each with its own scoring system. To facilitate this process, we introduce TBM Hunter—an accessible, web-based tool. This user-friendly platform provides a cost-free and efficient means to screen and assess potential TBMs within any given protein. TBM Hunter can handle individual proteins or lists of proteins simultaneously. Notably, our results demonstrate that TBM Hunter not only identifies known TBMs but also uncovers novel ones. In summary, our study offers an all-encompassing perspective on TBMs and presents an easy-to-use, precise, and free tool for identifying and evaluating potential TBMs in any protein, thereby enhancing research and drug development efforts focused on tankyrases.
Glutaredoxin in Rice Growth, Development, and Stress Resistance: Mechanisms and Research Advances
Authors: Zhai Rongrong , Ye Shenghai , Ye Jing , Wu Mingming , Zhu Guofu , Yu Faming , Wang Xingyu , Feng Yue , Zhang Xiaoming
Published: November 2023

Rice (Oryza sativa L.) is a staple food for more than half of the global population. Various abiotic and biotic stresses lead to accumulation of reactive oxygen species in rice, which damage macromolecules and signaling pathways. Rice has evolved a variety of antioxidant systems, including glutaredoxin (GRX), that protect against various stressors. A total of 48 GRX gene loci have been identified on 11 of the 12 chromosomes of the rice genome; none were found on chromosome 9. GRX proteins were classified into four categories according to their active sites: CPYC, CGFS, CC, and GRL. In this paper, we summarized the recent research advances regarding the roles of GRX in rice development regulation and response to stresses, and discussed future research perspectives related to rice production. This review could provide information for rice researchers on the current status of the GRX and serve as guidance for breeding superior varieties.
Studying the Functional Potential of Ground Ivy (Glechoma hederacea L.) Extract Using an In Vitro Methodology
Authors: Šeremet Danijela , Durgo Ksenija , Kosanović Jelena , Huđek Turković Ana , Mandura Jarić Ana , Vojvodić Cebin Aleksandra , Komes Draženka
Published: November 2023

Glechoma hederacea L., known as ground ivy, has a long history of use in folk medicine. The main bioactive compounds in ground ivy are polyphenolic compounds known for their potent antioxidant and antimicrobial activities and thus have high potential as functional ingredients against bacterial infections and the occurrence of chronic diseases associated with oxidative stress in the human body. The aim of the present study was to determine the biological activity of ground ivy extract on selected human cell lines, including hepatic (HepG2), tongue (CAL 27), gastric (AGS) and colon (Caco-2) cancer cell lines by evaluating cytotoxicity, formation of reactive oxygen species and genotoxicity. The antioxidant capacity of the extract was additionally evaluated using cellular model macromolecules of protein and DNA, bovine serum album and plasmid phiX174 RF1 DNA. The effect of ground ivy extract on representatives of human microflora, including L. plantarum, E. coli and S. aureus, was also studied. The cytotoxicity of the extract depended on the type of cells treated, and the pro-oxidant effect generally decreased with increasing exposure time. The most pronounced genoprotective effect against hydroxyl radical damage was monitored in model plasmid DNA and occurred at the highest tested concentration (0.25 mg mL−1), with 95.89% preservation of the supercoiled form of the plasmid. This concentration also had the most significant antioxidant activity on the model protein—14.01% more than the positive control prepared using Trolox. The ground ivy extract showed high antimicrobial potential against the pathogenic bacteria E. coli and S. aureus.
Genome-Wide Identification of Catalase Gene Family and the Function of SmCAT4 in Eggplant Response to Salt Stress
Authors: Shen Lei , Xia Xin , Zhang Longhao , Yang Shixin , Yang Xu
Published: November 2023

Salinity is an important abiotic stress, damaging plant tissues by causing a burst of reactive oxygen species (ROS). Catalase (CAT) enzyme coded by Catalase (CAT) genes are potent in reducing harmful ROS and hydrogen peroxide (H2O2) produced. Herein, we performed bioinformatics and functional characterization of four SmCAT genes, retrieved from the eggplant genome database. Evolutionary analysis CAT genes revealed that they are divided into subgroups I and II. The RT-qPCR analysis of SmCAT displayed a differential expression pattern in response to abiotic stresses. All the CAT proteins of eggplant were localized in the peroxisome, except for SmCAT4, which localized in the cytomembrane and nucleus. Silencing of SmCAT4 compromised the tolerance of eggplant to salt stress. Suppressed expression levels of salt stress defense related genes SmTAS14 and SmDHN1, as well as increase of H2O2 content and decrease of CAT enzyme activity was observed in the SmCAT4 silenced eggplants. Our data provided insightful knowledge of CAT gene family in eggplant. Positive regulation of eggplant response to salinity by SmCAT4 provides resource for future breeding programs.
Light-Driven Tetra- and Octa-β-substituted Cationic Zinc(II) Phthalocyanines for Eradicating Fusarium oxysporum Conidia
Authors: Lourenço Leandro M. O. , Cunha Ângela , Sierra-Garcia Isabel N.
Published: November 2023

Photodynamic inactivation (PDI) is an emerging therapeutic approach that can effectively inactivate diverse microbial forms, including vegetative forms and spores, while preserving host tissues and avoiding the development of resistance to the photosensitization procedure. This study evaluates the antifungal and sporicidal photodynamic activity of two water-soluble amphiphilic tetra- and octa-β-substituted zinc(II) phthalocyanine (ZnPc) dyes with dimethylaminopyridinium groups at the periphery (ZnPcs 1, 2) and their quaternized derivatives (ZnPcs 1a, 2a). Tetra(1, 1a)- and octa(2, 2a)-β-substituted zinc(II) phthalocyanines were prepared and assessed as photosensitizers (PSs) for their effects on Fusarium oxysporum conidia. Antimicrobial photoinactivation experiments were performed with each PS at 0.1, 1, 10, and 20 µM under white light irradiation at an irradiance of 135 mW·cm–2, for 60 min (light dose of 486 J·cm−2). High PDI efficiency was observed for PSs 1a, 2, and 2a (10 µM), corresponding to inactivation until the method’s detection limit. PS 1 (20 µM) also achieved a considerable reduction of >5 log10 in the concentration of viable conidia. The quaternized PSs (1a, 2a) showed better PDI performance than the non-quaternized ones (1, 2), even at the low concentration of 1 µM, and a light dose of 486 J·cm−2. These cationic phthalocyanines are potent photodynamic drugs for antifungal applications due to their ability to effectively inactivate resistant forms, like conidia, with low concentrations and reasonable energy doses.
A Biomimetic Multiparametric Assay to Characterise Anti-Amyloid Drugs
Authors: Smeralda Willy , Since Marc , Corvaisier Sophie , Fayolle Dimitri , Cardin Julien , Duprey Sylvain , Jourdan Jean-Pierre , Cullin Christophe , Malzert-Freon Aurélie
Published: November 2023

Alzheimer’s disease (AD) is the most widespread form of senile dementia worldwide and represents a leading socioeconomic problem in healthcare. Although it is widely debated, the aggregation of the amyloid β peptide (Aβ) is linked to the onset and progression of this neurodegenerative disease. Molecules capable of interfering with specific steps in the fibrillation process remain of pharmacological interest. To identify such compounds, we have set up a small molecule screening process combining multiple experimental methods (UV and florescence spectrometry, ITC, and ATR-FTIR) to identify and characterise potential modulators of Aβ1-42 fibrillation through the description of the biochemical interactions (molecule–membrane Aβ peptide). Three known modulators, namely bexarotene, Chicago sky blue and indomethacin, have been evaluated through this process, and their modulation mechanism in the presence of a biomembrane has been described. Such a well-adapted physico-chemical approach to drug discovery proves to be an undeniable asset for the rapid characterisation of compounds of therapeutic interest for Alzheimer’s disease. This strategy could be adapted and transposed to search for modulators of other amyloids such as tau protein.
YUCCA2 (YUC2)-Mediated 3-Indoleacetic Acid (IAA) Biosynthesis Regulates Chloroplast RNA Editing by Relieving the Auxin Response Factor 1 (ARF1)-Dependent Inhibition of Editing Factors in Arabidopsis thaliana
Authors: Li Zi-Ang , Li Yi , Liu Dan , Molloy David P. , Luo Zhou-Fei , Li Hai-Ou , Zhao Jing , Zhou Jing , Su Yi , Wang Ruo-Zhong , Huang Chao , Xiao Lang-Tao
Published: November 2023

Although recent research progress on the abundant C-to-U RNA editing events in plant chloroplasts and mitochondria has uncovered many recognition factors and their molecular mechanisms, the intrinsic regulation of RNA editing within plants remains largely unknown. This study aimed to establish a regulatory relationship in Arabidopsis between the plant hormone auxin and chloroplast RNA editing. We first analyzed auxin response elements (AuxREs) present within promoters of chloroplast editing factors reported to date. We found that each has more than one AuxRE, suggesting a potential regulatory role of auxin in their expression. Further investigation unveiled that the depletion of auxin synthesis gene YUC2 reduces the expression of several editing factors. However, in yuc2 mutants, only the expression of CRR4, DYW1, ISE2, and ECD1 editing factors and the editing efficiency of their corresponding editing sites, ndhD-2 and rps14-149, were simultaneously suppressed. In addition, exogenous IAA and the overexpression of YUC2 enhanced the expression of these editing factors and the editing efficiency at the ndhD-2 and rps14-149 sites. These results suggested a direct effect of auxin upon the editing of the ndhD-2 and rps14-149 sites through the modulation of the expression of the editing factors. We further demonstrated that ARF1, a downstream transcription factor in the auxin-signaling pathway, could directly bind to and inactivate the promoters of CRR4, DYW1, and ISE2 in a dual-luciferase reporter system, thereby inhibiting their expression. Moreover, the overexpression of ARF1 in Arabidopsis significantly reduced the expression of the three editing factors and the editing efficiency at the ndhD-2 and rps14-149 sites. These data suggest that YUC2-mediated auxin biosynthesis governs the RNA-editing process through the ARF1-dependent signal transduction pathway.
Comparative Transcriptomics and Metabolomics Analyses of Avicennia marina and Kandelia obovata under Chilling Stress during Seedling Stage
Authors: Wang Shu-Min , Wang You-Shao , Cheng Hao
Published: November 2023

One of the most productive ecosystems in the world, mangroves are susceptible to cold stress. However, there is currently insufficient knowledge of the adaptation mechanisms of mangrove plants in response to chilling stress. This study conducted a comparative analysis of transcriptomics and metabolomics to investigate the adaptive responses of Kandelia obovata (chilling-tolerant) and Avicennia marina (chilling-sensitive) to 5 °C. The transcriptomics results revealed that differentially expressed genes (DEGs) were mostly enriched in signal transduction, photosynthesis-related pathways, and phenylpropanoid biosynthesis. The expression pattern of genes involved in photosynthesis-related pathways in A. marina presented a downregulation of most DEGs, which correlated with the decrease in total chlorophyll content. In the susceptible A. marina, all DEGs encoding mitogen-activated protein kinase were upregulated. Phenylpropanoid-related genes were observed to be highly induced in K. obovata. Additionally, several metabolites, such as 4-aminobutyric acid, exhibited higher levels in K. obovata than in A. marina, suggesting that chilling-tolerant varieties regulated more metabolites in response to chilling. The investigation defined the inherent distinctions between K. obovata and A. marina in terms of signal transduction gene expression, as well as phenylpropanoid and flavonoid biosynthesis, during exposure to low temperatures.
Inferring the Regulatory Network of miRNAs on Terpene Trilactone Biosynthesis Affected by Environmental Conditions
Authors: Guo Ying , Qi Yongli , Feng Yangfan , Yang Yuting , Xue Liangjiao , El-Kassaby Yousry A. , Wang Guibin , Fu Fangfang
Published: November 2023

As a medicinal tree species, ginkgo (Ginkgo biloba L.) and terpene trilactones (TTLs) extracted from its leaves are the main pharmacologic activity constituents and important economic indicators of its value. The accumulation of TTLs is known to be affected by environmental stress, while the regulatory mechanism of environmental response mediated by microRNAs (miRNAs) at the post-transcriptional levels remains unclear. Here, we focused on grafted ginkgo grown in northwestern, southwestern, and eastern-central China and integrally analyzed RNA-seq and small RNA-seq high-throughput sequencing data as well as metabolomics data from leaf samples of ginkgo clones grown in natural environments. The content of bilobalide was highest among detected TTLs, and there was more than a twofold variation in the accumulation of bilobalide between growth conditions. Meanwhile, transcriptome analysis found significant differences in the expression of 19 TTL-related genes among ginkgo leaves from different environments. Small RNA sequencing and analysis showed that 62 of the 521 miRNAs identified were differentially expressed among different samples, especially the expression of miRN50, miR169h/i, and miR169e was susceptible to environmental changes. Further, we found that transcription factors (ERF, MYB, C3H, HD-ZIP, HSF, and NAC) and miRNAs (miR319e/f, miRN2, miRN54, miR157, miR185, and miRN188) could activate or inhibit the expression of TTL-related genes to participate in the regulation of terpene trilactones biosynthesis in ginkgo leaves by weighted gene co-regulatory network analysis. Our findings provide new insights into the understanding of the regulatory mechanism of TTL biosynthesis but also lay the foundation for ginkgo leaves’ medicinal value improvement under global change.
Advancements in 2D and 3D In Vitro Models for Studying Neuromuscular Diseases
Authors: Kim Haneul , Kim Gon Sup , Hyun Sang-Hwan , Kim Eunhye
Published: November 2023

Neuromuscular diseases (NMDs) are a genetically or clinically heterogeneous group of diseases that involve injury or dysfunction of neuromuscular tissue components, including peripheral motor neurons, skeletal muscles, and neuromuscular junctions. To study NMDs and develop potential therapies, remarkable progress has been made in generating in vitro neuromuscular models using engineering approaches to recapitulate the complex physical and biochemical microenvironments of 3D human neuromuscular tissues. In this review, we discuss recent studies focusing on the development of in vitro co-culture models of human motor neurons and skeletal muscles, with the pros and cons of each approach. Furthermore, we explain how neuromuscular in vitro models recapitulate certain aspects of specific NMDs, including amyotrophic lateral sclerosis and muscular dystrophy. Research on neuromuscular organoids (NMO) will continue to co-develop to better mimic tissues in vivo and will provide a better understanding of the development of the neuromuscular tissue, mechanisms of NMD action, and tools applicable to preclinical studies, including drug screening and toxicity tests.
Decentralised IOTA-Based Concepts of Digital Trust for Securing Remote Driving in an Urban Environment
Authors: Latvakoski Juhani , Kyllönen Vesa , Ronkainen Jussi
Published: November 2023

The novel contribution of this research is decentralised IOTA-based concepts of digital trust for securing remote driving in an urban environment. The conceptual solutions are studied and described, and respective experimental solutions are developed relying on digital identities, public key cryptography with a decentralised approach using decentralised identifiers (DIDs) and verifiable credentials (VCs), and an IOTA-based distributed ledger. The provided digital trust solutions were validated by executing them according to the remote driving scenario but with a simulated vehicle and simulated remote driving system. The hybrid simulation mainly focused on the validation of functional, causal temporal correctness, feasibility, and capabilities of the provided solutions. The evaluations indicate that the concepts of digital trust fulfil the purpose and contribute towards making remote driving more trustable. A supervisory stakeholder was used as a verifier, requiring a set of example verifiable credentials from the vehicle and the remote driver, and accepting them to the security control channel. The separation of control and data planes from each other was found to be a good solution because the delays caused by required security control can be limited to the initiation of the remote driving session without causing additional delays in the actual real-time remote driving control data flow. The application of the IOTA Tangle as the verifiable data registry was found to be sufficient for security control purposes. During the evaluations, the need for further studies related to scalability, application of wallets, dynamic trust situations, time-sensitive behaviour, and autonomous operations, as well as smart contract(s) between multiple stakeholders, were detected. As the next step of this research, the provided digital trust solutions will be integrated with a vehicle, remote driving system and traffic infrastructure for evaluation of the performance, reliability, scalability, and flexibility in real-world experiments of remote driving of an electric bus in an urban environment.
Assessing the Impact of Melanin Content on the Reliability of the Idylla™ BRAF Mutation Test
Authors: Parsons Sam D. , Murphy Kate , Finall Alison
Published: November 2023

Aims: This study aims to investigate the potential influence of melanin content on the performance of the Idylla™ BRAF Mutation Test. Specifically, we assess whether melanin levels in samples impact the test’s reliability, thereby validating its clinical utility in accelerating melanoma diagnosis and potentially improving patient prognosis. Methods: We conducted a retrospective analysis of 98 confirmed melanoma samples collected between February 2020 and November 2020. Formalin-fixed paraffin-embedded (FFPE) slides were evaluated by two independent observers using light microscopy to categorise samples into three groups based on melanin content (no, low, or high) following a standardised system. The samples underwent the Idylla™ BRAF Mutation Test and were compared with results obtained from next-generation sequencing (NGS). Results: Quantification cycle (Cq) values were utilised to assess for interference from melanin levels on the Idylla™ BRAF Mutation Test results. Statistical analyses revealed no significant differences in Cq values based on melanin content categories. Furthermore, analysis of polymerase chain reaction PCR curves did not indicate any notable influence of melanin. Discordant results with NGS are discussed. Conclusions: The study demonstrates that melanin content in samples does not significantly affect the performance of the Idylla™ BRAF Mutation Test. These results provide robust evidence supporting the confident application of the test in clinical settings, even for samples with high melanin content. The ability to obtain rapid on-site results holds promising potential in guiding timely and appropriate treatment decisions, thereby contributing to improved patient prognosis. What is already known on this topic—Prior research conducted by Petty et al. (2020) including 23 melanoma samples suggested that melanin does not significantly interfere with the Idylla™ BRAF Mutation Test by stating they were concordant with reference laboratory testing. What this study adds—This current study builds upon prior research with a larger sample size of 98. In addition to examining concordance between the Idylla™ BRAF Mutation Test and next generation sequencing, this study examines PCR curves and effect on Cq values, providing more robust evidence that melanin content in FFPE samples does not have a significant impact on the accuracy of the Idylla™ BRAF Mutation Test. How this study might affect research, practice or policy—The additional evidence base provided by this study is valuable for researchers, clinicians, and policymakers, as it supports the integration of the Idylla™ BRAF Mutation Test as a rapid and accurate method for detecting these mutations in melanoma patients.
COVID-19 and Supply Chain Disruption Management: A Behavioural Economics Perspective and Future Research Direction
Authors: Smith Chase , Fatorachian Hajar
Published: November 2023

The COVID-19 pandemic has been one of the most severe disruptions to normal life, impacting how businesses operate. The academic literature in the areas of supply chain and operations management has been trying to explain how this has affected decision-making in businesses. However, the existing literature has predominantly overlooked organisational culture and behavioural economic theories. This paper contends that considering the decisions made in supply chain disruption management involve groups and the individuals within them, the relevance of behavioural economic concepts becomes paramount. As such, the objective of this paper is to conduct an integrative literature review, utilising the purposive sampling method to explore the dearth of academic work connecting behavioural economic theories and organisational culture to supply chain disruption management. Additionally, the paper aims to offer guidelines for future research in this domain. Enhancing our comprehension of these domains concerning supply chain disruption management would empower firms to better anticipate their parties’ decisions, refine their decision-making models, and cultivate stronger relationships with suppliers and customers.
Structural Relaxation, Rejuvenation and Plasticity of Metallic Glasses: Microscopic Details from Anelastic Relaxation Spectra
Authors: Atzmon Michael , Ju Jong Doo , Lei Tianjiao
Published: November 2023

The lack of periodicity and long-range order poses significant challenges in explaining and modeling the properties of metallic glasses. Conventional modeling of nonexponential relaxation with stretched exponents leads to inconsistencies and rarely offers information on microscopic properties. Instead, using quasi-static anelastic relaxation, we have obtained relaxation-time spectra over >10 orders of magnitude of time for several metallic glasses. The spectra enable us to examine in microscopic detail the distribution of shear transformation zones and their properties. They reveal an atomically-quantized hierarchy of shear transformation zones, providing insights into the effect of structural relaxation and rejuvenation, the origin of plasticity and the mechanisms of the alpha and beta relaxation.
Processing and Properties of ZrB2-Copper Matrix Composites Produced by Ball Milling and Spark Plasma Sintering
Authors: Sulima Iwona , Boczkal Grzegorz
Published: November 2023

Copper matrix composites with zirconium diboride (ZrB2) were synthesised by ball milling and consolidated by Spark Plasma Sintering (SPS). Characterisations of the ball-milled composite powders were performed by scanning electron microscopy (SEM), X-ray diffraction, and measurement of the particle size distribution. The effect of the sintering temperature (1123 K, 1173 K, and 1223 K) and pressure (20 MPa and 35 MPa) on the density, porosity, and Young’s modulus was investigated. The relationship between the change of Orb content and physical, mechanical, and electrical properties was studied. Experimental data showed that the properties of Cu–Orb composites depended significantly on the SPS sintering conditions. The optimal sintering temperature was 1223 K with a pressure of 35 MPa. Composites exhibited a high degree of consolidation. For these materials, the apparent density was in the range of 93–97%. The results showed that the higher content of Orb in the copper matrix was responsible for the improvement in Young’s modulus and hardness with the reduction of the conductivity of sintered composites. The results showed that Young’s modulus and the hardness of the Cu 20% Orb composites were the highest, and were 165 GPa and 174 HV0.3, respectively. These composites had the lowest relative electrical conductivity of 17%.
Impact Resistance of Ultra-High-Performance Concrete Composite Structures
Authors: Ning Huijun , Ren Huiqi , Wang Wei , Nie Xiaodong
Published: November 2023

Ultra-high-performance concrete (UHPC) is a cement-based material with excellent impact resistance. Compared with traditional concrete, it possesses ultra-high strength, ultra-high toughness, and ultra-high durability, making it an ideal material for designing structures with impact resistance. The research on the impact resistance performance of UHPC and its composite structures is of great significance for the structural design of protective engineering projects. However, currently, there is still insufficient research on the impact resistance performance of UHPC composite structures. To study the impact resistance performance, experiments were conducted on UHPC targets using high-speed projectiles. The results were compared with impact tests on granite targets. The results indicated that when subjected to projectile impact, the UHPC targets exhibited smaller surface craters compared with the granite targets, while the penetration depth was lower in the granite targets. Afterwards, the process of a projectile impacting the UHPC composite structure was numerically simulated using ANSYS 16.0/LS-DYNA finite element software. The numerical simulation results of penetration depth and crater diameter were in good agreement with the experimental results, which indicates the rationality of the numerical model. Based on this, further analysis was carried out on the influence of impact velocity, impact angle, and reinforcement ratio on the penetration depth of the composite structure. The results show that the larger the incident angle or the smaller the velocity of the projectile is, the easier it is to deflect the projectile. There is a linear relationship between penetration depth and reinforcement ratio; as the reinforcement ratio increases, the penetration depth decreases significantly. This research is of great significance in improving the safety and reliability of key projects and also contributes to the application and development of ultra-high-performance materials in the engineering field.
Influence of Curing Temperature on the Strength of a Metakaolin-Based Geopolymer
Authors: Lopes Adelino , Lopes Sérgio , Pinto Isabel
Published: November 2023

The present work focuses on the further development of a new family of geopolymers obtained by the alkaline activation of a binder. The aim is to find a viable alternative to concrete that can be used in civil construction. Regarding the influence of the curing temperature on this type of mixture, the recommendations in the existing literature are different for fly ash, ground granulated blast-furnace slag, and metakaolin-based geopolymers. While for fly ash and slag, increasing the curing temperature above 60 °C is reported to be advantageous, for metakaolin geopolymers, the opposite is reported. In this context, the objective of this work is to evaluate the mechanical strength of several metakaolin-based geopolymer specimens subjected to different curing temperatures (10, 15, 20, 30, 40 and 50 °C). Furthermore, several stress-strain diagrams are also shown. Based on the results, we recommend using curing temperatures below 30 °C in order to avoid reducing the strength of metakaolin-based geopolymers. Curing at 50 °C, relative to room temperature, results in a reduction of more than 35% in flexural strength and a reduction of more than 60% in compressive strength. Regarding the behavior of the geopolymers, it was found that the strain, at the ultimate stress, is about 2 to 2.5 times the strain of an equivalent cement mortar.
Numerical and Experimental Study on Carbon Segregation in Shaped Billet of Medium Carbon Steel with Combined Electromagnetic Stirring
Authors: Li Pengchao , Zhang Guifang , Yan Peng , Tian Nan , Feng Zhenhua
Published: November 2023

Carbon segregation is the major and classical internal defect in the continuous casting process of carbon steel. Based on the combined electromagnetic stirring equipment for new billet in a steel plant, China, the influence of combined electromagnetic stirring (M-EMS + F-EMS) on the carbon segregation of 300 mm × 340 mm special-shaped billet was studied via numerical simulation and on-site industrialization tests. The Lorentz force and carbon solute distribution were simulated under different EMS parameters. The formation mechanism of the carbon segregation of medium carbon steel with different combined electromagnetic stirring processes was analyzed. The results show that: (1) with the combined action of “solute flushing” effect and gravity, the carbon concentration in the loose side of the medium carbon steel casting billet is gradually lower than the fixed side, while the carbon concentration on the fixed side gradually accumulates more; and (2) under the action of combined electromagnetic stirring, the segregation index of casting billet could be controlled to remain between 0.96–1.05 and shows an increasing change in solidification from the skin to the center. When the current and frequency of M-EMS are 250 A and 2.0 Hz and the F-EMS are 180 A and 8.0 Hz, the carbon segregation defects in the special-shaped (300 mm × 340 mm) casting billet can be significantly improved.
Reliability Optimization of the Honeycomb Sandwich Structure Based on A Neural Network Surrogate Model
Authors: Wei Zheng , Zhou Chunping , Zhang Feng , Zhou Changcong
Published: November 2023

Composite radomes are usually located in the nose of aircraft and are important structural components that protect radar antenna. The finite element model of a radome structure is developed and analyzed in this article. Single-objective deterministic and reliability optimization models based on the minimum total mass of the radome structure were established, and the layer thickness of each part of the honeycomb sandwich radome structure was considered a design variable. A multi-objective deterministic and reliability optimization model for a radome structure with a minimum total mass and maximum buckling critical load was established, and a particle swarm optimization algorithm was used to solve the problem. Our optimized results satisfied the constraints and utilization rate of materials, and structural safety was improved.
Analysing Semi-Supervised ConvNet Model Performance with Computation Processes
Authors: Neghawi Elie , Liu Yan
Published: November 2023

The rapid development of semi-supervised machine learning (SSML) algorithms has shown enhanced versatility, but pinpointing the primary influencing factors remains a challenge. Historically, deep neural networks (DNNs) have been used to underpin these algorithms, resulting in improved classification precision. This study aims to delve into the performance determinants of SSML models by employing post-hoc explainable artificial intelligence (XAI) methods. By analyzing the components of well-established SSML algorithms and comparing them to newer counterparts, this work redefines semi-supervised computation processes for both data preprocessing and classification. Integrating different types of DNNs, we evaluated the effects of parameter adjustments during training across varied labeled and unlabeled data proportions. Our analysis of 45 experiments showed a notable 8% drop in training loss and a 6.75% enhancement in learning precision when using the Shake-Shake26 classifier with the RemixMatch SSML algorithm. Additionally, our findings suggest a strong positive relationship between the amount of labeled data and training duration, indicating that more labeled data leads to extended training periods, which further influences parameter adjustments in learning processes.
Early- to Mid-Term Review of a Prospective, Multi-Center, International, Outcomes Study of an Anatomically Designed Implant with Posterior-Stabilized Bearing in Total Knee Arthroplasty
Authors: Kim Sung Eun , Ro Du Hyun , Lee Myung Chul , Cholewa Jason M.
Published: November 2023

Background and Objectives: National joint registries report higher total knee arthroplasty (TKA) revision rates in posterior-stabilized (PS) systems compared to non-posterior-stabilized designs. The purpose of this study was to investigate the implant survivorship and clinical outcomes of an anatomic implant with a PS bearing. Materials and Methods: An early- to mid-term follow-up of a prospective, multi-center, non-controlled outcomes study of patients who received primary TKA between November 2014 and June 2017 was performed. A total of 800 cases using PS bearings that were implanted in 664 patients were monitored post-operatively for their implant survivorship and adverse events for up to five years. The Knee Society Knee and Function scores, patient satisfaction, the five-dimensional European Quality of Life questionnaire, and range of motion (ROM) were evaluated pre-operatively and post-operatively at six weeks, six months, one year, two years, three years, and five years. Results: The mean follow-up period was 3.7 ± 1.3 years, and the three-year implant survival rate was 99.3% (95% CI: 98.4%, 99.7%) with five revisions during the five-year follow-up. Patient satisfaction was 96.1% at six weeks and increased to 99.3% at one year. All patient-reported outcome measures significantly (p < 0.0001) increased up to the one-year follow-up and then remained stable up to the five-year follow-up. Conclusions: This study supports the excellent survivorship and patient-reported outcomes of the Persona® Knee system using cemented, fixed bearing, posterior-stabilized components with minimal complications at early- to mid-term follow-up in an international Asian population. Ongoing observations are being performed to investigate the mid- to long-term survivorship and clinical outcomes associated with this knee system.
Percutaneous Gastrostomy Tube Placement under Quadratus Lumborum Block: A Case Report
Authors: Ose Gundega , Evansa Irina , Ivanovs Nikita , Zlobina Natalija , Vanags Indulis , Sabelnikovs Olegs
Published: November 2023

The quadratus lumborum block is a technique that is not widely applied in abdominal surgery. The influence of the mode of anesthesia on the outcome of polymorbid patients is a controversial issue in the medical literature. We report a case in which we performed a quadratus lumborum block type 2 on a woman who was admitted to Riga’s 1st hospital in need of gastrostomy, due to difficulty swallowing solid foods and liquids caused by hypopharynx carcinoma. On account of the patient’s difficult airway, general anesthesia was deemed unsafe for the patient, with a risk of patient death. Percutaneous gastrostomy tube placement under a quadratus lumborum block type 2 was performed successfully.
Study on UF PES Membranes Spray-Coated with Polymerizable Bicontinuous Microemulsion Materials for Low-Fouling Behavior
Authors: De Sneha , Heer Jonathan , Sankar Suwetha , Geiger Fabian , Gukelberger Ephraim , Galiano Francesco , Mancuso Raffaella , Gabriele Bartolo , Figoli Alberto , Hoinkis Jan
Published: November 2023

The low-fouling propensity of commercially available polyethersulfone (PES) membranes was studied after modification of the membrane surface via coating with polymerizable bicontinuous microemulsion (PBM) materials. The PBM coating was polymerized within 1 min using ultraviolet (UV) light exposure. It was detected on the PES membrane surface via attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy. The PBM coating led to an average 10% increase in the hydrophilicity of the PES membrane surface and an increase in total organic content (TOC) removal by more than 15%. Flux-step tests were conducted with model foulant comprising 100 mg L−1 humic acid (HA) solution to detect the onset of critical fouling, characterized by a rapid and substantial increase in TMP, and to compare the fouling propensity of commercially available PES membranes with PBM-coated membranes. The critical flux was found to be about 40% higher for PBM spray-coated membrane and 20% lower for PBM casting-coated membrane than the commercial PES membrane. This demonstrates the performance advantages of the thin PBM layer spray-coated on PES membrane compared to the thick casting-coated PBM layer. The study showcases the potential of PBM spray-coated membranes over commercial PES membranes for use in membrane bioreactors (MBR) for wastewater treatment systems with reduced maintenance over longer operation periods.
A Thermodynamic Perspective of Cancer Cells’ Volume/Area Expansion Ratio
Authors: Lucia Umberto , Fino Debora , Deisboeck Thomas S. , Grisolia Giulia
Published: November 2023

The constructal law is used to improve the analysis of the resonant heat transfer in cancer cells. The result highlights the fundamental role of the volume/area ratio and its role in cancer growth and invasion. Cancer cells seek to increase their surface area to facilitate heat dissipation; as such, the tumour expansion ratio declines as malignant cells start to migrate and the cancer expands locally and systemically. Consequently, we deduce that effective anticancer therapy should be based on the control of some ion transport phenomena in an effort to increase the volume/area ratio. This emphasises restricting the local and systemic spatial expansion of the tumour system and thus gives further credence to the superior role of novel anti-migratory and anti-invasive treatment strategies over conventional anti-proliferative options only.
Impact of Different Treatment Regimens and Timeframes in the Plasmatic Metabolic Profiling of Patients with Lung Adenocarcinoma
Authors: Madama Daniela , Carrageta David F. , Guerra-Carvalho Bárbara , Botelho Maria F. , Oliveira Pedro F. , Cordeiro Carlos R. , Alves Marco G. , Abrantes Ana M.
Published: November 2023

In recent years, the treatment of advanced non-small cell lung cancer (NSCLC) has suffered a variety of alterations. Chemotherapy (CTX), immunotherapy (IT) and tyrosine kinase inhibitors (TKI) have shown remarkable results. However, not all patients with NSCLC respond to these drug treatments or receive durable benefits. In this framework, metabolomics has been applied to improve the diagnosis, treatment, and prognosis of lung cancer and particularly lung adenocarcinoma (AdC). In our study, metabolomics was used to analyze plasma samples from 18 patients with AdC treated with CTX or IT via 1H-NMR spectroscopy. Relevant clinical information was gathered, and several biochemical parameters were also evaluated throughout the treatments. During the follow-up of patients undergoing CTX or IT, imaging control is recommended in order to assess the effectiveness of the therapy. This evaluation is usually performed every three treatments. Based on this procedure, all the samples were collected before the beginning of the treatment and after three and six treatments. The identified and quantified metabolites in the analyzed plasma samples were the following: isoleucine, valine, alanine, acetate, lactate, glucose, tyrosine, and formate. Multivariate/univariate statistical analyses were performed. Our data are in accordance with previous published results, suggesting that the plasma glucose levels of patients under CTX become higher throughout the course of treatment, which we hypothesize could be related to the tumor response to the therapy. It was also found that alanine levels become lower during treatment with CTX regimens, a fact that could be associated with frailty. NMR spectra of long responders’ profiles also showed similar results. Based on the results of the study, metabolomics can represent a potential option for future studies, in order to facilitate patient selection and the monitoring of therapy efficacy in treated patients with AdC. Further studies are needed to improve the prospective identification of predictive markers, particularly glucose and alanine levels, as well as confer guidance to NSCLC treatment and patient stratification, thus avoiding ineffective therapeutic strategies.
Design and Fabrication of a 3D-Printed Microfluidic Immunoarray for Ultrasensitive Multiplexed Protein Detection
Authors: Hiniduma Keshani , Bhalerao Ketki S. , De Silva Peyahandi I. Thilini , Chen Tianqi , Rusling James F.
Published: November 2023

Microfluidic technology has revolutionized device fabrication by merging principles of fluid dynamics with technologies from chemistry, physics, biology, material science, and microelectronics. Microfluidic systems manipulate small volumes of fluids to perform automated tasks with applications ranging from chemical syntheses to biomedical diagnostics. The advent of low-cost 3D printers has revolutionized the development of microfluidic systems. For measuring molecules, 3D printing offers cost-effective, time, and ease-of-designing benefits. In this paper, we present a comprehensive tutorial for design, optimization, and validation for creating a 3D-printed microfluidic immunoarray for ultrasensitive detection of multiple protein biomarkers. The target is the development of a point of care array to determine five protein biomarkers for aggressive cancers. The design phase involves defining dimensions of microchannels, reagent chambers, detection wells, and optimizing parameters and detection methods. In this study, the physical design of the array underwent multiple iterations to optimize key features, such as developing open detection wells for uniform signal distribution and a flap for covering wells during the assay. Then, full signal optimization for sensitivity and limit of detection (LOD) was performed, and calibration plots were generated to assess linear dynamic ranges and LODs. Varying characteristics among biomarkers highlighted the need for tailored assay conditions. Spike-recovery studies confirmed the assay’s accuracy. Overall, this paper showcases the methodology, rigor, and innovation involved in designing a 3D-printed microfluidic immunoarray. Optimized parameters, calibration equations, and sensitivity and accuracy data contribute valuable metrics for future applications in biomarker analyses.
Multifunctional Polymer-Metal Lattice Composites via Hybrid Additive Manufacturing Technology
Authors: He Liu , Wang Peiren , Wang Lizhe , Chen Min , Liu Haiyun , Li Ji
Published: November 2023

With increasing interest in the rapid development of lattice structures, hybrid additive manufacturing (HAM) technology has become a competent alternative to traditional solutions such as water jet cutting and investment casting. Herein, a HAM technology that combines vat photopolymerization (VPP) and electroless/electroplating processes is developed for the fabrication of multifunctional polymer-metal lattice composites. A VPP 3D printing process is used to deliver complex lattice frameworks, and afterward, electroless plating is employed to deposit a thin layer of nickel-phosphorus (Ni-P) conductive seed layer. With the subsequent electroplating process, the thickness of the copper layer can reach 40 μm within 1 h and the resistivity is around 1.9×10−8 Ω⋅m, which is quite close to pure copper (1.7 ×10−8 Ω⋅m). The thick metal shell can largely enhance the mechanical performance of lattice structures, including structural strength, ductility, and stiffness, and meanwhile provide current supply capability for electrical applications. With this technology, the frame arms of unmanned aerial vehicles (UAV) are developed to demonstrate the application potential of this HAM technology for fabricating multifunctional polymer-metal lattice composites.