bluuebunny/crossref_metadata_2025 huggingface dataset:
CHTHONIC DISRUPTION IN LYCOPHRON'SALEXANDRA
Authors: Warwick Celsiana
Published: March 2023

This paper argues that Lycophron'sAlexandrafollows earlier texts in presenting challenges to Agamemnon's power as metaphorical re-enactments of primordial theogonic conflicts between Zeus and the forces of chaos. TheAlexandrafigures Agamemnon as Zeus and portrays Achilles, Clytemnestra and Cassandra as chthonic monsters opposed to the Olympian order. Employing intertexts with epic and tragedy, the poem highlights these figures’ symbolic antagonism with Agamemnon–Zeus and their connections to each other. It presents a radically resystematized vision of the cosmos that champions the chthonic, the disordered and the feminine over the Olympian, the ordered and the masculine. Cassandra uses this backdrop to reinterpret her own story, inserting herself into the cosmogonic narrative as a resister of Olympian patriarchy who triumphs over masculine domination.
Sunscreening pigments shape the horizontal distribution of pendent hair lichens in the lower canopy of unmanaged coniferous forests
Authors: Gauslaa Yngvar , Goward Trevor
Published: March 2023

Hair lichens are distinctive for their capillary growth and typically arboreal occurrence, especially in temperate and boreal forests. They consist of two morphogroups based on cortical pigments: a brown-black group with fungal melanin and a pale yellow-green group with usnic acid. Here we test the hypothesis that these morphogroups are ecologically distinct and thus appropriately regarded as functional groups. We examine their respective horizontal occurrence in the lower canopy of 60-year-old conifer forests on a 250 m tall volcanic cone in south-central British Columbia. Trees on open south-facing slopes and near the summit were found to support mainly melanic hair lichens(BryoriaandNodobryoria), whereas more densely spaced trees on north-facing slopes and at the base had higher cover values of usnic lichens (especiallyAlectoria sarmentosaandRamalina thrausta). The cover of melanic hair lichens was strongly correlated with canopy openness but not for their usnic counterparts. We suggest that investment in light-absorbing melanic pigments is an extreme form of specialization for high light, favouring persistence in dry, sun-exposed canopies of otherwise cool forests. By contrast, the cortex of pendent usnic hair lichens appears to facilitate optimum light transmission to underlying photobionts in shaded sites, though at the cost of sensitivity to light in open habitats, especially in rather dry regions.
Do the negative symptoms of schizophrenia reflect reduced responsiveness to reward? Examination using a reward prediction error (RPE) task
Authors: Fuentes-Claramonte Paola , Garcia-Leon Maria Angeles , Salgado-Pineda Pilar , Ramiro Núria , Soler-Vidal Joan , Torres Maria Llanos , Cano Ramon , Argila-Plaza Isabel , Panicali Francesco , Sarri Carmen , Jaurrieta Núria , Sánchez Manel , Boix-Quintana Ester , Albacete Auria , Maristany Teresa , Sarró Salvador , Raduà Joaquim , McKenna Peter J. , Salvador Raymond , Pomarol-Clotet Edith
Published: March 2023

Background

A leading theory of the negative symptoms of schizophrenia is that they reflect reduced responsiveness to rewarding stimuli. This proposal has been linked to abnormal (reduced) dopamine function in the disorder, because phasic release of dopamine is known to code for reward prediction error (RPE). Nevertheless, few functional imaging studies have examined if patients with negative symptoms show reduced RPE-associated activations.

Methods

Matched groups of DSM-5 schizophrenia patients with high negative symptom scores (HNS, N = 27) or absent negative symptoms (ANS, N = 27) and healthy controls (HC, N = 30) underwent fMRI scanning while they performed a probabilistic monetary reward task designed to generate a measure of RPE.

Results

In the HC, whole-brain analysis revealed that RPE was positively associated with activation in the ventral striatum, the putamen, and areas of the lateral prefrontal cortex and orbitofrontal cortex, among other regions. Group comparison revealed no activation differences between the healthy controls and the ANS patients. However, compared to the ANS patients, the HNS patients showed regions of significantly reduced activation in the left ventrolateral and dorsolateral prefrontal cortex, and in the right lingual and fusiform gyrus. HNS and ANS patients showed no activation differences in ventral striatal or midbrain regions-of-interest (ROIs), but the HNS patients showed reduced activation in a left orbitofrontal cortex ROI.

Conclusions

The findings do not suggest that a generalized reduction of RPE signalling underlies negative symptoms. Instead, they point to a more circumscribed dysfunction in the lateral frontal and possibly the orbitofrontal cortex.
Work and earning in the nineteenth century: Townley Colliery as a case study
Authors: Solomon Guy
Published: March 2023

This article demonstrates the varied and unpredictable nature of earning in the nineteenth century. Using 12,000 fortnightly pay entries from Townley Main Colliery in the north-east of England as a case study, it explores the extent to which the availability of work fluctuated between years, and how workers reacted to this phenomenon. It then considers the frequency with which these individuals undertook the work which was available, and discusses the implications for our understanding of the length of the working year.
Plea Leniency and Prosecution Centredness in China's Criminal Process
Authors: He Xin
Published: March 2023

China's criminal proceedings have been recognized as being “investigation centred.” I argue that the rise of the Plea Leniency System has led to “prosecution centredness.” Analysis of the operation and consequences of plea leniency shows how the procuratorate has overshadowed the police and further marginalized the courts. In plea leniency, the defendant has little chance of being acquitted and the legal profession provides little defence. While this paradigm shift signals further leniency in criminal justice, rights protections make way for efficiency and crime control. As such, plea leniency has profound implications for the operation of the criminal justice apparatuses, defendants, defence lawyers, and the mode of crime control in China.
Antiquities trafficking in conflict countries: A crime-mapping approach
Authors: Suber David Leone , Mazzali Luca , Heins Guido Thomas , Matteoni Pietro , Tiberio Marco , Zolghadriha Sanaz , Bradford Ben
Published: March 2023

Studies on antiquities trafficking have often been overshadowed by research looking at the trafficking of human beings, drugs, and weapons, a fact partly motivated by the arguably higher relevance and greater security implications involved in these other forms of illicit trade. However, the past decade of conflicts in the Middle East has revived an interest in the study of antiquities trafficking networks.1 The association between the growing size of the illicit antiquities market and conflicts in the region did not go unnoticed by crime scientists and criminologists looking deeper at the relation between the trafficking of antiquities and transnational organized crime.2
Current Evidence for Infection Prevention and Control Interventions in Emergency Medical Services: A Scoping Review
Authors: Jenkins J. Lee , Hsu Edbert B. , Zhang Allen , Wilson Lisa M. , Russell Anna , Bass Eric B.
Published: March 2023

Objectives:

The aim of this review was to summarize current evidence from the United States on the effectiveness of practices and interventions for preventing, recognizing, and controlling occupationally acquired infectious diseases in Emergency Medical Service (EMS) clinicians.

Report and Methods:

PubMed, Embase, CINAHL, and SCOPUS were searched from January 1, 2006 through March 15, 2022 for studies in the United States that involved EMS clinicians and firefighters, reported on one or more workplace practices or interventions that prevented or controlled infectious diseases, and included outcome measures. Eleven (11) observational studies reported on infection prevention and control (IPC) practices providing evidence that hand hygiene, standard precautions, mandatory vaccine policies, and on-site vaccine clinics are effective. Less frequent handwashing (survey-weight adjusted odds ratio [OR] 4.20; 95% confidence interval [CI], 1.02 to 17.27) and less frequent hand hygiene after glove use (survey-weight adjusted OR 10.51; 95% CI, 2.54 to 43.45) were positively correlated with nasal colonization of Methicillin-resistant Staphylococcus aureus (MRSA). Lack of personal protective equipment (PPE) or PPE breach were correlated with higher severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) seropositivity (unadjusted risk ratio [RR] 4.2; 95% CI, 1.03 to 17.22). Workers were more likely to be vaccinated against influenza if their employer offered the vaccine (unadjusted OR 3.3; 95% CI, 1.3 to 8.3). Active, targeted education modules for H1N1 influenza were effective at increasing vaccination rates and the success of on-site vaccine clinics.

Conclusions:

Evidence from the United States exists on the effectiveness of IPC practices in EMS clinicians, including hand hygiene, standard precautions, mandatory vaccine policies, and vaccine clinics. More research is needed on the effectiveness of PPE and vaccine acceptance.
Finding Common Ground: Innovation and Diffusion across Political Science and Public Management Research
Authors: Berry Frances S.
Published: March 2023

It is quite an honor to be selected for this John Gaus Award Lecture by a committee of my peers. I want to thank my husband, Bill Berry—my partner for 48 years and sometimes coauthor—and certainly my children, Katie Berry and David Berry, who have diverted my attention from policy studies for 35 years now and made life much more enjoyable by their personalities. I especially want to thank my many coauthors who have kept research and writing an engaging endeavor while we have developed friendships that will last a lifetime.
Undergraduates and Political Science Research: Insights from Research Assistants in a Minority-Serving Institution Lab
Authors: Velásquez Zabdi R. , Esmail Jasmine , Stoltz Harry , Turkmen Fulya Felicity , Kuffuor Owura , Burnett John , Pizarro Naia , Aguilar Kimberly , Wang Allison , Kozak Alex , Park Eun-A , Dionne Kim Yi
Published: March 2023

This study examines undergraduate research experiences at a minority-serving institution (MSI) in a political science laboratory. Students contributed to projects in a collaborative research lab at the University of California Riverside that involves undergraduate and graduate students in projects related to health and politics. Adopting a participatory approach to research, the study’s research participants also are coauthors who co-created the research protocols; collected the data; transcribed, coded, and analyzed the data; and wrote up the findings. Our analysis of 12 in-depth interviews with current and former undergraduate research assistants (RAs) found that their work in the lab challenged their perceptions of what research is and what it means to do research; shaped their path to pursue graduate studies; developed their social and professional skills; and offered an inclusive and humanizing experience with graduate students and faculty members. Challenges that the RAs mentioned included time management, bureaucratic accounting and payroll procedures, and feelings of self-doubt; the lab’s culture of inclusion and independence mitigated some of these challenges. Our findings align with the scholarly literature that suggests collaborative research opportunities can have beneficial outcomes, particularly for students from groups that are underrepresented in doctoral programs.
Experiences of being a severely ill parent of dependent children receiving care at home: Hopes and challenges
Authors: Holm Maja , Lövgren Malin , Alvariza Anette , Eklund Rakel , Kreicbergs Ulrika
Published: March 2023

Objectives

Multiple studies have focused on severely ill patients in the role as parents of dependent children, yet few have explored the thoughts and feelings within this group during palliative home care.

Methods

This qualitative study derives from a pilot intervention study, the Family Talk Intervention (FTI), in specialized palliative home care. The FTI is a support program with the main goal to increase family communication about illness-related topics. The study is based on field notes from 104 sessions with 20 parents taken by an interventionist during intervention delivery. The field notes were analyzed using the principles of qualitative content analysis.

Results

The field notes revealed several challenging situations for parents with severe illness. A major issue was how to find ways to talk to their children about their illness and prognosis. The parents expressed guilt for being unable to fulfill their roles as parents and partners. Existing family conflicts had escalated with the illness, according to some parents. Despite being affected by illness, parents tried to have hope, if only for small things – such as a period of ordinary family life.

Significance of results

Severely ill parents in specialized palliative home care seek support regarding how to communicate and stay connected to their roles in the family, which is a struggle when a parent is cared for at home, while it in contrast may promote normality and hope. Communication with children is vital and needs to be brought to the attention of health-care professionals. A family-centered focus, involving both parents and children, should be embraced.
Systemic Financial Risk Arising From Residential Flood Losses
Authors: Thomson Hope , Zeff Harrison B. , Kleiman Rachel , Sebastian Antonia , Characklis Gregory W.
Published: March 2023

Direct damage from flooding at residential properties has typically been categorized as insured, with liabilities accruing to insurers, or uninsured, with costs accruing to property owners. However, residential flooding can also expose lenders and local governments to financial risk, though the distribution of this risk is not well understood. Flood losses are not limited to direct damages, but also include indirect effects such as decreases in property values, which can be substantial, though are rarely well quantified. The combination of direct damage and property value decrease influences rates of mortgage default and property abandonment in the wake of a flood, creating financial risk. In this research, property‐level data on sales, mortgages, and insurance claims are used in combination with machine learning techniques and geostatistical methods to provide estimates of flood losses that are then utilized to evaluate the risk of default and abandonment in eastern North Carolina following Hurricane Florence (2018). Within the study area, Hurricane Florence generated $366M in observed insured damages and an estimated $1.77B in combined uninsured damages and property value decreases. Property owners, lenders, and local governments were exposed to an additional $562M in potential losses due to increased rates of default and abandonment. Areas with lower pre‐flood property values were exposed to greater risk than areas with higher valued properties. Results suggest more highly resolved estimates of a flooding event's systemic financial risk may be useful in developing improved flood resilience strategies.
Airborne Absolute Gravimetry With a Quantum Sensor, Comparison With Classical Technologies
Authors: Bidel Y. , Zahzam N. , Bresson A. , Blanchard C. , Bonnin A. , Bernard J. , Cadoret M. , Jensen T. E. , Forsberg R. , Salaun C. , Lucas S. , Lequentrec‐Lalancette M. F. , Rouxel D. , Gabalda G. , Seoane L. , Vu D. T. , Bruinsma S. , Bonvalot S.
Published: March 2023

We report an airborne gravity survey with an absolute gravimeter based on atom interferometry and two relative gravimeters: a classical LaCoste&Romberg (L&R) and a novel iMAR strapdown Inertial Measurement Unit. We estimated measurement errors for the quantum gravimeter ranging from 0.6 to 1.3 mGal depending on the flight conditions and the filtering used. Similar measurement errors are obtained with iMAR strapdown gravimeter, but the long‐term stability is five times worse. The traditional L&R platform gravimeter shows larger measurement errors (3–4 mGal). Airborne measurements have been compared to marine, land, and altimetry‐derived gravity data. We obtain a good agreement for the quantum gravimeter with standard deviations and means on differences below or equal to 2 mGal. This study confirms the potential of quantum technology for absolute airborne gravimetry, which is particularly interesting for mapping shallow water or mountainous areas and for linking ground and satellite measurements with homogeneous absolute referencing.
A Scheme for Representing Aromatic Secondary Organic Aerosols in Chemical Transport Models: Application to Source Attribution of Organic Aerosols Over South Korea During the KORUS‐AQ Campaign
Authors: Brewer J. F. , Jacob D. J. , Jathar S. H. , He Y. , Akherati A. , Zhai S. , Jo D. S. , Hodzic A. , Nault B. A. , Campuzano‐Jost P. , Jimenez J. L. , Park R. J. , Oak Y. J. , Liao H.
Published: March 2023

We present a new volatility basis set (VBS) representation of aromatic secondary organic aerosol (SOA) for atmospheric chemistry models by fitting a statistical oxidation model with aerosol microphysics (SOM‐TOMAS) to results from laboratory chamber experiments. The resulting SOM‐VBS scheme also including previous work on SOA formation from semi‐ and intermediate volatile organic compounds (S/IVOCs) is implemented in the GEOS‐Chem chemical transport model and applied to simulation of observations from the Korea‐United States Air Quality Study (KORUS‐AQ) field campaign over South Korea in May–June 2016. Our SOM‐VBS scheme can simulate the KORUS‐AQ organic aerosol (OA) observations from aircraft and surface sites better than the default schemes used in GEOS‐Chem including for vertical profiles, diurnal cycle, and partitioning between hydrocarbon‐like OA and oxidized OA. Our results confirm the important contributions of oxidized primary OA and aromatic SOA found in previous analyses of the KORUS‐AQ data and further show a large contribution from S/IVOCs. Model source attribution of OA in surface air over South Korea indicates one third from domestic anthropogenic emissions, with a large contribution from toluene and xylenes, one third from external anthropogenic emissions, and one third from natural emissions.
Correlative light-electron microscopy using small gold nanoparticles as single probes
Authors: Pope Iestyn , Tanner Hugh , Masia Francesco , Payne Lukas , Arkill Kenton Paul , Mantell Judith , Langbein Wolfgang , Borri Paola , Verkade Paul
Published: March 2023

Correlative light-electron microscopy (CLEM) requires the availability of robust probes which are visible both in light and electron microscopy. Here we demonstrate a CLEM approach using small gold nanoparticles as a single probe. Individual gold nanoparticles bound to the epidermal growth factor protein were located with nanometric precision background-free in human cancer cells by light microscopy using resonant four-wave mixing (FWM), and were correlatively mapped with high accuracy to the corresponding transmission electron microscopy images. We used nanoparticles of 10 nm and 5 nm radius, and show a correlation accuracy below 60 nm over an area larger than 10 µm size, without the need for additional fiducial markers. Correlation accuracy was improved to below 40 nm by reducing systematic errors, while the localisation precision is below 10 nm. Polarisation-resolved FWM correlates with nanoparticle shapes, promising for multiplexing by shape recognition in future applications. Owing to the photostability of gold nanoparticles and the applicability of FWM microscopy to living cells, FWM-CLEM opens up a powerful alternative to fluorescence-based methods.
Focal adhesion is associated with lithium response in bipolar disorder: evidence from a network-based multi-omics analysis
Authors: Niemsiri Vipavee , Rosenthal Sara Brin , Nievergelt Caroline M. , Maihofer Adam X. , Marchetto Maria C. , Santos Renata , Shekhtman Tatyana , Alliey-Rodriguez Ney , Anand Amit , Balaraman Yokesh , Berrettini Wade H. , Bertram Holli , Burdick Katherine E. , Calabrese Joseph R. , Calkin Cynthia V. , Conroy Carla , Coryell William H. , DeModena Anna , Eyler Lisa T. , Feeder Scott , Fisher Carrie , Frazier Nicole , Frye Mark A. , Gao Keming , Garnham Julie , Gershon Elliot S. , Goes Fernando S. , Goto Toyomi , Harrington Gloria J. , Jakobsen Petter , Kamali Masoud , Kelly Marisa , Leckband Susan G. , Lohoff Falk W. , McCarthy Michael J. , McInnis Melvin G. , Craig David , Millett Caitlin E. , Mondimore Francis , Morken Gunnar , Nurnberger John I. , Donovan Claire O’ , Øedegaard Ketil J. , Ryan Kelly , Schinagle Martha , Shilling Paul D. , Slaney Claire , Stapp Emma K. , Stautland Andrea , Tarwater Bruce , Zandi Peter P. , Alda Martin , Fisch Kathleen M. , Gage Fred H. , Kelsoe John R.
Published: March 2023

Lithium (Li) is one of the most effective drugs for treating bipolar disorder (BD), however, there is presently no way to predict response to guide treatment. The aim of this study is to identify functional genes and pathways that distinguish BD Li responders (LR) from BD Li non-responders (NR). An initial Pharmacogenomics of Bipolar Disorder study (PGBD) GWAS of lithium response did not provide any significant results. As a result, we then employed network-based integrative analysis of transcriptomic and genomic data. In transcriptomic study of iPSC-derived neurons, 41 significantly differentially expressed (DE) genes were identified in LR vs NR regardless of lithium exposure. In the PGBD, post-GWAS gene prioritization using the GWA-boosting (GWAB) approach identified 1119 candidate genes. Following DE-derived network propagation, there was a highly significant overlap of genes between the top 500- and top 2000-proximal gene networks and the GWAB gene list (Phypergeometric = 1.28E–09 and 4.10E–18, respectively). Functional enrichment analyses of the top 500 proximal network genes identified focal adhesion and the extracellular matrix (ECM) as the most significant functions. Our findings suggest that the difference between LR and NR was a much greater effect than that of lithium. The direct impact of dysregulation of focal adhesion on axon guidance and neuronal circuits could underpin mechanisms of response to lithium, as well as underlying BD. It also highlights the power of integrative multi-omics analysis of transcriptomic and genomic profiling to gain molecular insights into lithium response in BD.
Empirically driven transdiagnostic stages in the development of mood, anxiety and psychotic symptoms in a cohort of youth followed from birth
Authors: Ratheesh Aswin , Hammond Dylan , Gao Caroline , Marwaha Steven , Thompson Andrew , Hartmann Jessica , Davey Christopher , Zammit Stanley , Berk Michael , McGorry Patrick , Nelson Barnaby
Published: March 2023

Staging models with transdiagnostic validity across mood, psychotic, and anxiety disorders could advance early intervention efforts as well as our understanding of the common underpinnings of such psychopathology. However, there are few well-supported operationalisations for such transdiagnostic models, particularly in community-based samples. We aimed to explore the inter-relationships among mood, psychotic, and anxiety symptom stages, and their common risk factors to develop data-informed transdiagnostic stages. We included participants from the Avon Longitudinal Study of Parents and Children (ALSPAC), a prospective ongoing birth cohort study. We developed operational thresholds for stages of depressive, hypomanic, anxiety, and psychotic symptoms based on the existing literature, refined further by expert consensus. We selected 1b level as the primary stage or outcome of interest. This represents moderate symptoms that are likely to be associated with the onset of the need for clinical mental health care. We used questionnaire and clinic data completed by young people ages 18 and 21 years. We used descriptive methods and network analyses to examine the overlap among Stage 1b psychopathology. We then examined the patterns of relationships between several risk factors and 1b stages using logistic regressions. Among 3269 young people with data available to determine all symptom stages, 64.3% were female and 96% Caucasian. Descriptive and network analyses indicated that 1b level depressive, anxiety, and psychotic symptom stages were inter-related while hypomania was not. Similarly, anxiety, depressive, and psychotic 1b stages were associated with the female sex, more emotional and behavioral difficulties in early adolescence, and life events in late adolescence. Hypomania was not related to any of these risk factors. Given their inter-relationships and similar risk factors, anxiety, psychotic and depressive, symptoms could be combined to form a transdiagnostic stage in this cohort. Such empirical transdiagnostic stages could help with prognostication and indicated prevention in youth mental health.
Multiple myeloma with acute light chain cast nephropathy
Authors: Leung Nelson , Rajkumar S. Vincent
Published: March 2023

Light chain cast nephropathy (LCCN) is a leading cause of acute kidney injury (AKI) in patients with multiple myeloma (MM) and is now defined as a myeloma defining event. While the long-term prognosis has improved with novel agents, short-term mortality remains significantly higher in patients with LCCN especially if the renal failure is not reversed. Recovery of renal function requires a rapid and significant reduction of the involved serum free light chain. Therefore, proper treatment of these patients is of the utmost importance. In this paper, we provide an algorithm for treatment of MM patients who present with biopsy-proven LCCN or in those where other causes of AKI have been ruled out. The algorithm is based on data from randomized trial whenever possible. When trial data is not available, our recommendations is based on non-randomized data and expert opinions on best practices. We recommend that all patients should enroll in a clinical trial if available prior to resorting to the treatment algorithm we outlined.
RIP3-mediated microglial necroptosis promotes neuroinflammation and neurodegeneration in the early stages of diabetic retinopathy
Authors: Huang Zijing , Liang Jiajian , Chen Shaolang , Ng Tsz Kin , Brelén Marten E. , Liu Qingping , Yang Rucui , Xie Biyao , Ke Shuping , Chen Weiqi , Huang Dingguo
Published: March 2023

Diabetic retinopathy (DR) is a leading cause of blindness that poses significant public health concerns worldwide. Increasing evidence suggests that neuroinflammation plays a key role in the early stages of DR. Microglia, long-lived immune cells in the central nervous system, can become activated in response to pathological insults and contribute to retinal neuroinflammation. However, the molecular mechanisms of microglial activation during the early stages of DR are not fully understood. In this study, we used in vivo and in vitro assays to investigate the role of microglial activation in the early pathogenesis of DR. We found that activated microglia triggered an inflammatory cascade through a process called necroptosis, a newly discovered pathway of regulated cell death. In the diabetic retina, key components of the necroptotic machinery, including RIP1, RIP3, and MLKL, were highly expressed and mainly localized in activated microglia. Knockdown of RIP3 in DR mice reduced microglial necroptosis and decreased pro-inflammatory cytokines. Additionally, blocking necroptosis with the specific inhibitor GSK-872 improved retinal neuroinflammation and neurodegeneration, as well as visual function in diabetic mice. RIP3-mediated necroptosis was activated and contributed to inflammation in BV2 microglia under hyperglycaemic conditions. Our data demonstrate the importance of microglial necroptosis in retinal neuroinflammation related to diabetes and suggest that targeting necroptosis in microglia may be a promising therapeutic strategy for the early stages of DR.
Targeting Aurora-A inhibits tumor progression and sensitizes thyroid carcinoma to Sorafenib by decreasing PFKFB3-mediated glycolysis
Authors: Jingtai Zhi , Linfei Hu , Yuyang Qian , Ning Kang , Xinwei Yun , Xin Wang , Xianhui Ruan , Dongmei Huang , Weiwei Yang , Xiangrui Meng , Tianze Zhu , Wei Wang , Xiangqian Zheng
Published: March 2023

Thyroid cancer (TC) is the most common endocrine tumor, amongst which anaplastic thyroid carcinoma (ATC) is the most deadly. Aurora-A usually functions as oncogenes, and its inhibitor Alisertib exerts a powerful antitumor effect in various tumors. However, the mechanism of Aurora-A in regulating TC cell energy supply remains unclear. In the present study, we demonstrated the antitumor effect of Alisertib and an association between high Aurora-A expression and shorter survival. Multi-omics data and in vitro validation data suggested that Aurora-A induced PFKFB3-mediated glycolysis to increase ATP supply, which significantly upregulated the phosphorylation of ERK and AKT. Furthermore, the combination of Alisertib and Sorafenib had a synergistic effect, further confirmed in xenograft models and in vitro. Collectively, our study provides compelling evidence of the prognostic value of Aurora-A expression and suggests that Aurora-A upregulates PFKFB3-mediated glycolysis to enhance ATP supply and promote TC progression. Combining Alisertib with Sorafenib has huge prospects for application in treating advanced thyroid carcinoma.
Genetic Creutzfeldt‒Jakob disease with 5-octapeptide repeats presented as frontotemporal dementia
Authors: Hamada Shinsuke , Takahashi-Iwata Ikuko , Satoh Katsuya , Kitamoto Tetsuyuki , Mizusawa Hidehiro , Moriwaka Fumio , Yabe Ichiro
Published: March 2023

The N-terminus of the PRNP gene normally contains a 5-octapeptide repeat (R1-R2-R2-R3-R4), and insertions at this locus can cause hereditary prion diseases. In the present study, we found a 5-octapeptide repeat insertion (5-OPRI) in a sibling case of frontotemporal dementia. Consistent with previous literature, 5-OPRI rarely met the diagnostic criteria for Creutzfeldt‒Jakob disease (CJD). We propose 5-OPRI as a suspected causative mutation for early-onset dementia, especially the frontotemporal type.
Muscle cell-type diversification is driven by bHLH transcription factor expansion and extensive effector gene duplications
Authors: Cole Alison G. , Jahnel Stefan M. , Kaul Sabrina , Steger Julia , Hagauer Julia , Denner Andreas , Murguia Patricio Ferrer , Taudes Elisabeth , Zimmermann Bob , Reischl Robert , Steinmetz Patrick R. H. , Technau Ulrich
Published: March 2023

Animals are typically composed of hundreds of different cell types, yet mechanisms underlying the emergence of new cell types remain unclear. Here we address the origin and diversification of muscle cells in the non-bilaterian, diploblastic sea anemone Nematostella vectensis. We discern two fast and two slow-contracting muscle cell populations, which differ by extensive sets of paralogous structural protein genes. We find that the regulatory gene set of the slow cnidarian muscles is remarkably similar to the bilaterian cardiac muscle, while the two fast muscles differ substantially from each other in terms of transcription factor profiles, though driving the same set of structural protein genes and having similar physiological characteristics. We show that anthozoan-specific paralogs of Paraxis/Twist/Hand-related bHLH transcription factors are involved in the formation of fast and slow muscles. Our data suggest that the subsequent recruitment of an entire effector gene set from the inner cell layer into the neural ectoderm contributes to the evolution of a novel muscle cell type. Thus, we conclude that extensive transcription factor gene duplications and co-option of effector modules act as an evolutionary mechanism underlying cell type diversification during metazoan evolution.
High-entropy alloy nanopatterns by prescribed metallization of DNA origami templates
Authors: Xie Mo , Fang Weina , Qu Zhibei , Hu Yang , Zhang Yichi , Chao Jie , Shi Jiye , Wang Lihua , Wang Lianhui , Tian Yang , Fan Chunhai , Liu Huajie
Published: March 2023

High-entropy multimetallic nanopatterns with controlled morphology, composition and uniformity hold great potential for developing nanoelectronics, nanophotonics and catalysis. Nevertheless, the lack of general methods for patterning multiple metals poses a limit. Here, we develop a DNA origami-based metallization reaction system to prescribe multimetallic nanopatterns with peroxidase-like activities. We find that strong coordination between metal elements and DNA bases enables the accumulation of metal ions on protruding clustered DNA (pcDNA) that are prescribed on DNA origami. As a result of the condensation of pcDNA, these sites can serve as nucleation site for metal plating. We have synthesized multimetallic nanopatterns composed of up to five metal elements (Co, Pd, Pt, Ag and Ni), and obtained insights on elemental uniformity control at the nanoscale. This method provides an alternative pathway to construct a library of multimetallic nanopatterns.
An electron-hole rich dual-site nickel catalyst for efficient photocatalytic overall water splitting
Authors: Yan Xiaoqing , Xia Mengyang , Liu Hanxuan , Zhang Bin , Chang Chunran , Wang Lianzhou , Yang Guidong
Published: March 2023

Photocatalysis offers an attractive strategy to upgrade H2O to renewable fuel H2. However, current photocatalytic hydrogen production technology often relies on additional sacrificial agents and noble metal cocatalysts, and there are limited photocatalysts possessing overall water splitting performance on their own. Here, we successfully construct an efficient catalytic system to realize overall water splitting, where hole-rich nickel phosphides (Ni2P) with polymeric carbon-oxygen semiconductor (PCOS) is the site for oxygen generation and electron-rich Ni2P with nickel sulfide (NiS) serves as the other site for producing H2. The electron-hole rich Ni2P based photocatalyst exhibits fast kinetics and a low thermodynamic energy barrier for overall water splitting with stoichiometric 2:1 hydrogen to oxygen ratio (150.7 μmol h−1H2and 70.2 μmol h−1O2produced per 100 mg photocatalyst) in a neutral solution. Density functional theory calculations show that the co-loading in Ni2P and its hybridization with PCOS or NiS can effectively regulate the electronic structures of the surface active sites, alter the reaction pathway, reduce the reaction energy barrier, boost the overall water splitting activity. In comparison with reported literatures, such photocatalyst represents the excellent performance among all reported transition-metal oxides and/or transition-metal sulfides and is even superior to noble metal catalyst.
Characterizing brain dynamics during ketamine-induced dissociation and subsequent interactions with propofol using human intracranial neurophysiology
Authors: Tian Fangyun , Lewis Laura D. , Zhou David W. , Balanza Gustavo A. , Paulk Angelique C. , Zelmann Rina , Peled Noam , Soper Daniel , Santa Cruz Mercado Laura A. , Peterfreund Robert A. , Aglio Linda S. , Eskandar Emad N. , Cosgrove G. Rees , Williams Ziv M. , Richardson R. Mark , Brown Emery N. , Akeju Oluwaseun , Cash Sydney S. , Purdon Patrick L.
Published: March 2023

Ketamine produces antidepressant effects in patients with treatment-resistant depression, but its usefulness is limited by its psychotropic side effects. Ketamine is thought to act via NMDA receptors and HCN1 channels to produce brain oscillations that are related to these effects. Using human intracranial recordings, we found that ketamine produces gamma oscillations in prefrontal cortex and hippocampus, structures previously implicated in ketamine’s antidepressant effects, and a 3 Hz oscillation in posteromedial cortex, previously proposed as a mechanism for its dissociative effects. We analyzed oscillatory changes after subsequent propofol administration, whose GABAergic activity antagonizes ketamine’s NMDA-mediated disinhibition, alongside a shared HCN1 inhibitory effect, to identify dynamics attributable to NMDA-mediated disinhibition versus HCN1 inhibition. Our results suggest that ketamine engages different neural circuits in distinct frequency-dependent patterns of activity to produce its antidepressant and dissociative sensory effects. These insights may help guide the development of brain dynamic biomarkers and novel therapeutics for depression.
Factors for optimizing intervention programs for cognition in older adults: the value of exergames
Authors: Perrot Alexandra , Maillot Pauline
Published: March 2023

This review presents factors that could optimize the effectiveness of an intervention program on cognitive health in older adults. Combined, multi-dimensional and interactive programs appear to be relevant. On one hand, for the characteristics to be implemented in the physical dimension of a program, multimodal interventions stimulating the aerobic pathway and muscle strengthening during the solicitation of gross motor activities, seem to be interesting. On the other hand, regarding the cognitive dimension of a program, complex and variable cognitive stimuli appear to hold the greatest promise for generating cognitive benefits and the broadest transfers to untrained tasks. The field of video games also brings interesting enrichment through the gamification of situations and the feeling of immersion. However, some gray areas remain to be clarified, notably the ideal response dose, the balance between physical and cognitive solicitation and the programs’ customization.
Impact of a Mn-oxidizing bacterial strain on the dissolution and browning of a Mn-bearing potash-lime silicate glass
Authors: Valbi Valentina , Perez Anne , Verney-Carron Aurélie , Rossano Stéphanie
Published: March 2023

This work aims to investigate the role played by a model Mn-oxidizing bacterial strain and its exudates on the alteration of Mn-bearing potash-lime silicate glasses representative of medieval stained glass windows. Two model glasses, with or without manganese, were prepared and used for abiotic and Pseudomonas putida inoculated dissolution experiments. Results show that the presence of P. putida slows down the dissolution kinetics while changing the dissolution stoichiometry. In biotic experiments, the acidification of the solution at the beginning of the experiment favors the release of K. After a few days, a drop in Mn and P in solution is observed, retained by bacterial cells. Reciprocally, the amount of glass influences bacterial behavior. The more glass, the faster the bacterial population increases in size and produces siderophore. In the presence of the Mn-bearing glass, siderophore production is followed by the formation of brown phases, identified as Mn oxides.
A corpus of CO2 electrocatalytic reduction process extracted from the scientific literature
Authors: Wang Ludi , Gao Yang , Chen Xueqing , Cui Wenjuan , Zhou Yuanchun , Luo Xinying , Xu Shuaishuai , Du Yi , Wang Bin
Published: March 2023

The electrocatalytic CO2reduction process has gained enormous attention for both environmental protection and chemicals production. Thereinto, the design of new electrocatalysts with high activity and selectivity can draw inspiration from the abundant scientific literature. An annotated and verified corpus made from massive literature can assist the development of natural language processing (NLP) models, which can offer insight to help guide the understanding of these underlying mechanisms. To facilitate data mining in this direction, we present a benchmark corpus of 6,086 records manually extracted from 835 electrocatalytic publications, along with an extended corpus with 145,179 records in this article. In this corpus, nine types of knowledge such as material, regulation method, product, faradaic efficiency, cell setup, electrolyte, synthesis method, current density, and voltage are provided by either annotating or extracting. Machine learning algorithms can be applied to the corpus to help scientists find new and effective electrocatalysts. Furthermore, researchers familiar with NLP can use this corpus to design domain-specific named entity recognition (NER) models.
Evaporative destabilization of a salt crust with branched pattern formation
Authors: Licsandru G. , Noiriel C. , Duru P. , Geoffroy S. , Abou-Chakra A. , Prat M.
Published: March 2023

The impact of salt crust formation over porous media on water evaporation is an important issue in relation with the water cycle, agriculture, building sciences and more. The salt crust is not a simple accumulation of salt crystals at the porous medium surface but undergoes complex dynamics with possible air gap formation between the crust and the porous medium surface. We report on experiments that allow to identify various crust evolution regimes depending on the competition between evaporation and vapor condensation. The various regimes are summarized in a diagram. We focus on the regime where dissolution–precipitation processes lead to the upward displacement of the salt crust and the generation of a branched pattern. It is shown that the branched pattern results from the crust upper surface destabilization whereas the crust lower surface remains essentially flat. We show that the resulting branched efflorescence salt crust is heterogeneous with a greater porosity in the salt fingers. This leads to the preferential drying of the salt fingers followed by a period in which the crust morphology change only occurs in the salt crust lower region. The salt crust eventually tends toward a frozen state where no visible change occurs in the salt crust morphology, but without blocking the evaporation. These findings provide in-depth insights into the salt crust dynamics and pave the way for the better understanding of the impact of efflorescence salt crusts on evaporation and the development of predictive models.
Revisiting the baby schema by a geometric morphometric analysis of infant facial characteristics across great apes
Authors: Kawaguchi Yuri , Nakamura Koyo , Tajima Tomoyuki , Waller Bridget M.
Published: March 2023

Infants across species are thought to exhibit specific facial features (termed the “baby schema”, such as a relatively bigger forehead and eyes, and protruding cheeks), with an adaptive function to induce caretaking behaviour from adults. There is abundant empirical evidence for this in humans, but, surprisingly, the existence of a baby schema in non-human animals has not been scientifically demonstrated. We investigated which facial characteristics are shared across infants in five species of great apes: humans, chimpanzees, bonobos, mountain gorillas, and Bornean orangutans. We analysed eight adult and infant faces for each species (80 images in total) using geometric morphometric analysis and machine learning. We found two principal components characterizing infant faces consistently observed across species. These included (1) relatively bigger eyes located lower in the face, (2) a rounder and vertically shorter face shape, and (3) an inverted triangular face shape. While these features are shared, human infant faces are unique in that the second characteristic (round face shape) is more pronounced, whereas the third (inverted triangular face shape) is less pronounced than other species. We also found some infantile features only found in some species. We discuss future directions to investigate the baby schema using an evolutionary approach.
Manufacturing carbon storage sintered body using microwave-selective and high-speed heating techniques
Authors: Kashimura K. , Oshita A. , Miyata T. , Segawa S. , Yokawa H. , Tendo K. , Kurooka K.
Published: March 2023

Microwave sintering of fly ash samples with large amounts of unburned carbon and CaCO3 was examined in this study. To this end, CaCO3 was mixed with fly ash sintered body to fix CO2. The decomposition of CaCO3 was observed when the raw material was heated to 1000 °C using microwave irradiation; however, a sintered body containing aragonite was obtained when the raw material was heated to 1000 °C with added water. Further, carbides in the fly ash could be selectively heated by controlling the microwave irradiation. The microwave magnetic field created a temperature gradient of 100 °C in a narrow region of 2.7 μm or less in the sintered body, and it helped suppress the CaCO3 decomposition in the mixture during sintering. By storing water in the gas phase before spreading, CaCO3, which is difficult to sinter using conventional heating, can be sintered without decomposing.
Impact of COVID-19 pandemic on emergency medical system and management strategies in patients with acute coronary syndrome
Authors: Saiin Kohei , Takenaka Sakae , Nagai Toshiyuki , Takahashi Akinori , Mizuguchi Yoshifumi , Konishi Takao , Anzai Toshihisa , Hotta Daisuke , Kamigaki Mitsunori , Yamazaki Seiji , Fujita Tsutomu , Yamashita Takehiro , Kawahatsu Kandoh , Suzuki Takashi , Nozaki Yoichi , Sakurada Taku , Takenaka Takashi , Igarashi Yasumi , Makino Takao ,   The Sapporo city Acute Coronary Syndrome Network
Published: March 2023

The global coronavirus disease-2019 (COVID-19) pandemic is associated with reduced rate of percutaneous coronary intervention (PCI). However, there were a few data showing how emergency medical system (EMS) and management strategies for acute coronary syndrome (ACS) changed during the pandemic. We sought to clarify changes on characteristics, treatments, and in-hospital mortality of patients with ACS transported via EMS between pre- and post-pandemic. We examined consecutive 656 patients with ACS admitted to Sapporo City ACS Network Hospitals between June 2018 and November 2021. The patients were divided into pre- and post-pandemic groups. The number of ACS hospitalizations declined significantly during the pandemic (proportional reduction 66%, coefficient −0.34, 95% CI −0.50 to −0.18, p < 0.001). The median time from an EMS call to hospital was significantly longer in post-pandemic group than in pre-pandemic group (32 [26–39] vs. 29 [25–36] min, p = 0.008). There were no significant differences in the proportion of patients with ACS receiving PCI, and in-hospital mortality between the groups. The COVID-19 pandemic had a significant impact on EMS and management in patients with ACS. Although a significant decline was observed in ACS hospitalizations, the proportion of patients with ACS receiving emergency PCI remained during the pandemic.