bluuebunny/crossref_metadata_2025 huggingface dataset:
Multi-Residue Method for the Analysis of Stilbene Estrogens in Milk
Authors: Guan Tianzhu , Sun Yonghai , Wang Yongjun , Li Zhuolin , Li Tiezhu , Hou Ligang
Published: February 2019

The rapid analysis of stilbene estrogens is crucially important in the environment, food and health sectors, but quantitation of lower detection limit for stilbene estrogens persists as a severe challenge. We herein described a homologous and sensitive fluorescence polarization (FP) assay based on estrogen receptor α ligand binding domain (ER-LBD) to monitor stilbene estrogens in milk. Under optimal conditions, the half maximal inhibitory concentrations (IC50) of the FP assay were 9.27 nM, 12.94 nM, and 22.38 nM for hexestrol, dienestrol and diethylstilbestrol, respectively. And the corresponding limits of detection (LOD) values were 2.94 nM, 2.89 nM, and 3.12 nM. Finally, the assay was applied to determine the stilbenes in milk samples where the mean recoveries ranged from 95.76% to 112.78% and the coefficients of variation (CV) below 12.00%. Furtherly, we have focused our study on high cross-reactivity phenomena by using two in silico approaches, including molecular docking analysis and topology analysis. Overall, docking results show that several residues in the hydrophobic pocket produce hydrophobic interactions with the tested drug molecules, which contribute to the stability of their binding. In this paper, we conclude that the FP method is suitable for the rapid detection of stilbenes in milk samples, requiring no expensive analytical equipment or time-consuming sample preparation. This work offers a practical approach that applies bioscience technology in food safety testing and improves analytical speed and laboratory efficiency.
Parkin Interacts with Apoptosis-Inducing Factor and Interferes with Its Translocation to the Nucleus in Neuronal Cells
Authors: Guida Marianna , Zanon Alessandra , Montibeller Luigi , Lavdas Alexandros A. , Ladurner Judith , Pischedda Francesca , Rakovic Aleksandar , Domingues Francisco S. , Piccoli Giovanni , Klein Christine , Pramstaller Peter P. , Hicks Andrew A. , Pichler Irene
Published: February 2019

Mutations in the PRKN gene (encoding parkin) have been linked to the most frequent known cause of recessive Parkinson’s disease (PD), and parkin dysfunction represents a risk factor for sporadic PD. Parkin is widely neuroprotective through different cellular pathways, as it protects dopaminergic neurons from apoptosis in a series of cellular and animal models of PD. The mitochondrial protein apoptosis-inducing factor (AIF) is an important cell death effector, which, upon cellular stress in many paradigms, is redistributed from the mitochondria to the nucleus to function as a proapoptotic factor, mostly independent of caspase activity, while in normal mitochondria it functions as an antiapoptotic factor. AIF is known to participate in dopaminergic neuron loss in experimental PD models and in patients with PD. We, therefore, investigated possible crosstalk between parkin and AIF. By using immunoprecipitation and proximity ligation assays, we demonstrated a physical interaction between the two proteins. Nuclear AIF translocation was significantly reduced by parkin expression in neuroblastoma SH-SY5Y cells after exposure to an apoptogenic stimulus. These results were confirmed in primary murine cortical neurons, which showed a higher nuclear translocation of AIF in parkin-deficient neurons upon an excitotoxic stimulus. Our results indicate that the interaction of parkin with AIF interferes with the nuclear translocation of AIF, which might contribute to the neuroprotective activity of parkin.
Map-Based Cloning and Functional Analysis of YE1 in Rice, Which Is Involved in Light-Dependent Chlorophyll Biogenesis and Photoperiodic Flowering Pathway
Authors: Peng Youlin , Zou Ting , Li Lamei , Tang Shiwen , Li Qiao , Zhang Jie , Chen Yongjun , Wang Xuechun , Yang Guotao , Hu Yungao
Published: February 2019

Light is one of the most important environmental factors that affect many aspects of plant growth, including chlorophyll (Chl) synthesis and flowering time. Here, we identified a rice mutant, yellow leaf and early flowering (ye1), and characterized the gene YE1 by using a map-based cloning method. YE1 encodes a heme oxygenase, which is localized to the chloroplasts. YE1 is expressed in various green tissues, especially in leaves, with a diurnal-rhythmic expression pattern, and its transcripts is also induced by light during leaf-greening. The mutant displays decreased Chl contents with less and disorderly thylakoid lamellar layers in chloroplasts, which reduced the photosynthesis rate. The early flowering phenotype of ye1 was not photoperiod-sensitive. Furthermore, the expression levels of Chl biosynthetic genes were downregulated in ye1 seedlings during de-etiolation responses to light. We also found that rhythmic expression patterns of genes involved in photoperiodic flowering were altered in the mutant. Based on these results, we infer that YE1 plays an important role in light-dependent Chl biogenesis as well as photoperiodic flowering pathway in rice.
Foreword to the Special Issue: “Towards the Multilingual Web of Data”
Authors: McCrae John P. , Gracia Jorge
Published: February 2019

We are pleased to introduce this special issue on the topic of “Towards the Multilingual Web of Data”, which we feel is a timely and valuable topic in our increasingly multilingual and interconnected world [...]
The 5W’s for Control as Part of Industry 4.0: Why, What, Where, Who, and When—A PID and MPC Control Perspective
Authors: Maxim Anca , Copot Dana , Copot Cosmin , Ionescu Clara M.
Published: February 2019

The advent of Industry 4.0 (I4.0) has pushed technology beyond its physical limits, making the process prone to errors and poorer performance. Whether it is about smart manufacturing where mass customization is envisaged, or collaborative human–robot engineering systems, the pyramid of process operation has changed to a matrix form and control is the backbone of all process elements. The paper gives a concise guideline as to how, when, where, and what to apply when it comes to choosing the most suitable control strategy as a function of multi-parameter objective optimization. Both proportional-integral-derivative (PID) and model predictive control (MPC) control are addressed in this context.
Endovascular Therapy for Tandem Occlusion in Acute Ischemic Stroke: Intravenous Thrombolysis Improves Outcomes
Authors: Pikija Slaven , Magdic Jozef , Sztriha Laszlo K. , Killer-Oberpfalzer Monika , Bubel Nele , Lukic Anita , Sellner Johann
Published: February 2019

Ischemic stroke related to tandem internal carotid and middle cerebral artery (TIM) occlusion is a challenging condition where endovascular treatment (EVT) is an emerging revascularization option. The identification of factors influencing clinical outcomes can assist in creating appropriate therapeutic algorithms for such patients. This study aimed to evaluate prognostic factors in the context of EVT for TIM occlusion. We performed a retrospective study of consecutive patients with TIM occlusion admitted within 6 h from symptom onset to two tertiary stroke centers. We recorded the etiology of stroke, clinical deficits at stroke onset and discharge, details of EVT, final infarct volume (FIV), in-hospital mortality, and outcome at three months. Among 73 patients with TIM occlusion, 53 were treated with EVT. The median age was 75.9 years (interquartile range (IQR) 64.6–82.6), with the most common etiology of cardioembolism (51.9%). Intravenous thrombolysis with tissue-plasminogen activator (t-PA) was performed in the majority (69.8%) of cases. EVT achieved successful recanalization with a thrombolysis in cerebral infarction (TICI) grade of 2b or 3 in 67.9%. A good outcome (modified Rankin score of 0–2 at three months) was observed in 37.7%. After adjustment for age, the National Institutes of Health Stroke Scale (NIHSS) at admission, and success of recanalization, smaller final infarct volume (odds ratio (OR) 0.021 for FIV above 25th percentile (95% CI 0.001–0.332, p = 0.005)) and administration of intravenous t-PA (OR 12.04 (95% CI 1.004–144.392, p = 0.049)) were associated with a good outcome at three months. Our study demonstrates that bridging with t-PA is associated with improved outcomes in the setting of tandem ICA and MCA occlusions treated with EVT and should therefore not be withheld in eligible patients.
The Relationship between Platelet Count and Host Gut Microbiota: A Population-Based Retrospective Cross-Sectional Study
Authors: Yoon Hee-Young , Kim Han-Na , Lee Su Hwan , Kim Soo Jung , Chang Yoosoo , Ryu Seungho , Shin Hocheol , Kim Hyung-Lae , Lee Jin Hwa
Published: February 2019

Platelet count reflects the severity and prognosis of multiple diseases. Additionally, alterations in gut microbiota have been linked to several chronic diseases. The purpose of this study was to investigate the association between gut microbiota and platelet count. We selected 1268 subjects with fecal 16S RNA gene sequencing data from a Healthcare Screening Center cohort. Based on the third quartile of platelets (277 × 109/L), we compared the gut microbiota between the upper quartile (n = 321) and lower three quartiles groups (n = 947). The upper quartile group had lower alpha diversity based on observed amplicon sequence variants (q = 0.004) and phylogenetic index (q < 0.001) than the lower three quartiles group. Significant differences were also found in the weighted UniFrac distance (q = 0.001) and Jaccard dissimilarity (q = 0.047) beta diversity measures between the two groups. Compared with the lower three quartiles group, the upper quartile group exhibited decreased relative abundances of the genus Faecalibacterium, which was also inversely correlated with the platelet count. Increased platelet count was associated with reduced diversity in gut microbiota and lower abundances of Faecalibacterium with beneficial gut bacteria spices F. prausnitzii, suggesting that an increased platelet count, even within normal range, may adversely affect gut microbial diversity and composition.
Corrosion Investigation of Reinforced Concrete Based on Piezoelectric Smart Materials
Authors: Shi Weihua , Chen Ying , Liu Peng , Xu Dongyu
Published: February 2019

An embedded piezoelectric transducer was developed for monitoring the corrosion process of reinforcement bars in concrete based on the piezoelectric impedance technique. The electrochemical method was employed to accelerate the corrosion process of the reinforcement bar with relative mass loss of 0.5–10%, and the resistance spectra of the piezoelectric transducers were investigated to assess the corrosion process. The results show that the corrosion process of the reinforcement bar has significant influence on the resistance spectra of the piezoelectric transducers. Statistical parameters were used to intuitively evaluate the corrosion evolution based on variations of the resistance spectra. The corrosion process of reinforcement bar in concrete can be classified into three periods; that is, the initial period when the relative mass loss is less than 2%, the developing period at a relative mass loss of 2–4%, and the rapid corrosion period when the relative mass loss is higher than 4%.
Temperature Field Numerical Analysis Mode and Verification of Quenching Heat Treatment Using Carbon Steel in Rotating Laser Scanning
Authors: Hung Tsung-Pin , Hsu Chao-Ming , Tsai Hsiu-An , Chen Shuo-Ching , Liu Zong-Rong
Published: February 2019

Temperature history and hardening depth are experimentally characterized in the rotational laser hardening process for an AISI 1045 medium carbon steel specimen. A three-dimensional finite element model is proposed to predict the temperature field distribution and hardening zone area. The laser temperature field is set up for an average distribution and scanned along a circular path. Linear motion also takes place alongside rotation. The prediction of hardening area can be increased by increasing the rotational radius, which in turn raises the processing efficiency. A good agreement is found between the experimental characterized hardness value and metallographic composition. The uniformity of the hardening area decreases with increasing laser scanning speed. The increased laser power input could help to expand the hardening depth.
First-Principles Study of the Electronic, Vibrational Properties and Anharmonic Effects of Some Si-Based Type-II Binary Clathrates
Authors: Xue Dong , Myles Charley W.
Published: February 2019

Electronic, vibrational, and anharmonic studies on some binary clathrate AxSi136 (A = Na, K, Rb, Cs; 0 < x ≤ 24) are theoretically presented. The Fermi energy lies in the range of 1.1 eV to 1.4 eV for NaxSi136 and increases as stoichiometry (x) is tuned from 8 to 12 to 16. The determined isotropic “Mexican-hat” shape of the guest-host potential describing Na motion in the Si28 cage indicates the “off-center” position when the temperature is elevated beyond zero. Accordingly, the calculated Na “off-center” displacements correlate well with the X-Ray Diffraction (XRD) data (0.4 Å–0.5 Å) for a similar composition range (0 < x < 24). The lack of first-principles analysis on quartic anharmonicity motivates us to initiate a self-consistent model to examine the temperature-dependent rattling frequency Ω(T) of the guest (Na, Rb). The predicted values of Ω(T) for Na24Si136 at 300 K are significantly higher (approximately six times larger) than the value at absolute zero, which contrasts with the case of Rb8Si136. Moreover, underestimation of the isotropic atomic displacement parameter Uiso is caused by the temperature-dependent quartic anharmonicity of Na, and this discrepancy might be offset by the square of the “off-center” displacement.
Stochastic Constitutive Model of Isotropic Thin Fiber Networks Based on Stochastic Volume Elements
Authors: Mansour Rami , Kulachenko Artem , Chen Wei , Olsson Mårten
Published: February 2019

Thin fiber networks are widely represented in nature and can be found in man-made materials such as paper and packaging. The strength of such materials is an intricate subject due to inherited randomness and size-dependencies. Direct fiber-level numerical simulations can provide insights into the role of the constitutive components of such networks, their morphology, and arrangements on the strength of the products made of them. However, direct mechanical simulation of randomly generated large and thin fiber networks is characterized by overwhelming computational costs. Herein, a stochastic constitutive model for predicting the random mechanical response of isotropic thin fiber networks of arbitrary size is presented. The model is based on stochastic volume elements (SVEs) with SVE size-specific deterministic and stochastic constitutive law parameters. The randomness in the network is described by the spatial fields of the uniaxial strain and strength to failure, formulated using multivariate kernel functions and approximate univariate probability density functions. The proposed stochastic continuum approach shows good agreement when compared to direct numerical simulation with respect to mechanical response. Furthermore, strain localization patterns matched the one observed in direct simulations, which suggests an accurate prediction of the failure location. This work demonstrates that the proposed stochastic constitutive model can be used to predict the response of random isotropic fiber networks of arbitrary size.
Optimization of Friction Behavior Characteristics by Coating with Solid Lubricants
Authors: Dodun Oana , Nagîţ Gheorghe , Hriţuc Adelina , Slătineanu Laurenţiu
Published: February 2019

Increasing the lifetime of machine elements whose operation involves the development of friction processes and diminishing energy losses by friction can be achieved by using solid lubricants. In this regard, a method applied to improve the friction behavior of machine elements is electrostatic coating of the surfaces of interest with polyester layers that include particles of solid lubricants, such as molybdenum disulfide or graphite. Experimental research was conducted to highlight the influence of normal force, the concentration of solid lubricant particles in polyester, and specific sliding between surfaces involved in the friction process on the deposited layer’s lifetime and on the friction coefficient. Grey analysis was employed to identify sets of input factors that would lead to the most convenient values of the lifetime and energy friction losses when using polyester layers that incorporate molybdenum or graphite particles. Specialized software was elaborated in a MATLAB environment to use the grey relational analysis in identifying the optimal values of the process input factors for distinct weights of the output parameters.
A Subclass of Bi-Univalent Functions Based on the Faber Polynomial Expansions and the Fibonacci Numbers
Authors: Altınkaya Şahsene , Yalçın Sibel , Çakmak Serkan
Published: February 2019

In this investigation, by using the Komatu integral operator, we introduce the new class of bi-univalent functions based on the rule of subordination. Moreover, we use the Faber polynomial expansions and Fibonacci numbers to derive bounds for the general coefficient of the bi-univalent function class.
Inhibitory Effects of Sodium Alginate on Hepatic Steatosis in Mice Induced by a Methionine- and Choline-Deficient Diet
Authors: Kawauchi Shoji , Horibe Sayo , Sasaki Naoto , Tanahashi Toshihito , Mizuno Shigeto , Hamaguchi Tsuneo , Rikitake Yoshiyuki
Published: February 2019

Nonalcoholic steatohepatitis (NASH) progresses from nonalcoholic fatty liver disease (NAFLD); however, efficacious drugs for NASH treatment are lacking. Sodium alginate (SA), a soluble dietary fiber extracted from brown algae, could protect the small intestine from enterobacterial invasion. NASH pathogenesis has been suggested to be associated with enterobacterial invasion, so we examined the effect of SA on methionine- and choline-deficient (MCD) diet-induced steatohepatitis in mice (the most widely-used model of NASH). The mice (n = 31) were divided into three groups (mice fed with regular chow, MCD diet, and MCD diet premixed with 5% SA) for 4 and 8 weeks. The MCD diet increased lipid accumulation and inflammation in the liver, the NAFLD Activity Score and hepatic mRNA expression of tumor necrosis factor-α and collagen 1α1, and induced macrophage infiltration. Villus shortening, disruption of zonula occludens-1 localization and depletion of mucus production were observed in the small intestine of the MCD-group mice. SA administration improved lipid accumulation and inflammation in the liver, and impaired barrier function in the small intestine. Collectively, these results suggest that SA is useful for NASH treatment because it can prevent hepatic inflammation and fatty degeneration by maintaining intestinal barrier function.
Formation of Surface Depression during Continuous Casting of High-Al TRIP Steel
Authors: Cui Heng , Zhang Kaitian , Wang Zheng , Chen Bin , Liu Baisong , Qing Jing , Li Zhijun
Published: February 2019

High aluminum transformation-induced plasticity (TRIP) steels offer a unique combination of high tensile strength and ductility, high impact energy absorption and good formability. The surface of the slab is prone to depressions and longitudinal cracks during continuous casting due to the high Al content in steels. Surface depressions of the 1.35 wt.% Al-TRIP steel slab in a steel works were investigated by scanning electronic microscopy (SEM) and mold fluxes with different Al2O3/SiO2 ratios were researched by thermodynamic calculations and high-temperature static balance experiments. The results show that some micro-cracks were distributed along the grain boundary in the surface depression of the slab. Inclusions containing K and Na, which were probably from mold flux, were found in the depression samples. Meanwhile, the components of reactive mold flux showed significant variation in their chemical composition during the continuous casting process of the Al-TRIP steel. A large number of depressions and irregular oscillation marks on the Al-TRIP steel slab surface were generated due to serious deterioration in the physical properties of the mold flux. Since the TRIP steel is a typical hypo-peritectic steel, the overly large thermal contraction and volume contraction during initial solidification is the intrinsic cause of surface depression. The change of mold flux properties during casting aggravates the formation of depressions.
Covellite (CuS) Production from a Real Acid Mine Drainage Treated with Biogenic H2S
Authors: Silva Patricia Magalhães Pereira , Lucheta Adriano Reis , Bitencourt José Augusto Pires , Carmo Andre Luiz Vilaça do , Cuevas Ivan Patricio Ñancucheo , Siqueira José Oswaldo , Oliveira Guilherme Corrêa de , Alves Joner Oliveira
Published: February 2019

Acid Mine Drainage (AMD) is an environmental problem associated with mining activities, which resulted from the exposure of sulfur bearing materials to oxygen and water. AMD is a pollution source due to its extreme acidity, high concentration of sulfate, and soluble metals. Biological AMD treatment is one alternative to couple environmental amelioration for valuable dissolved metals recovery, as a new source of raw materials. Covellite (CuS) particles were synthetized from an AMD sample collected in a Brazilian copper mine, after 48 and 96 h of exposure to hydrogen sulfide (H2S) produced in a bioreactor containing acidophilic sulfate reducing bacteria (SRB). The time of exposure affected the morphology, nucleation, and size of CuS crystals. CuS crystals synthetized after 96 h of H2S exposure showed better ordination as indicated by sharp and intense diffractograms obtained by X-ray diffraction (XRD), and the predominance of placoid sheets with hexagonal habit structure as observed by scanning electrons microscopy (SEM). Energy dispersive X-ray fluorescence (EDXRF) spectrometry indicated a Cu:S molar ratio in agreement with CuS. Granulometric analysis demonstrated that 90% of CuS particles were less than 22 µm size. AMD biological treatment is a potential economical CuS recovery option for metallurgical process chain incorporation, or new industrial applications, since the alteration of synthesis conditions can produce different crystal forms with specific characteristics.
Simulation and Experiment of Manufacturing Process for Structural Aluminum Parts with Hard to Plastic Forming
Authors: Jin Chul Kyu
Published: February 2019

A process comprising a hot extrusion process and a warm forging process was designed to form an umbrella-shaped aluminum structural component with a high degree of difficulty for the plastic forming method. A circular cylindrical part was extruded with a hot extrusion process, and then an embossing part was produced with a warm forging process. The formability and the maximum load required for forming were then determined using a forming analysis program. The hot extrusion process was executed at 450 °C under the extrusion speed at 6 mm/s, while the warm forging process was executed at 260 °C under the forging speed at 150 mm/s. The simulation results showed that the load required for hot extrusion was 1019 ton, while the load required for the warm forging was 534 ton. The umbrella-shaped part was manufactured by using a 1600 ton capacity press. The graphite lubricant was coated on the mold as well as the material. A forming experiment was performed under the same condition with the simulation condition. The portion where extrusion was done became elliptical with the α-Al phase elongated towards extrusion direction. Whereas, the α-Al phase became circular in the forged portion. The tensile strength value was found as 345 MPa, while elongation rate was 12%. Meanwhile, Vickers hardness value at the extruded portion was 105 HV, and it was 110 HV at the forged portion.
A Study on the Wettability of Ion-Implanted Stainless and Bearing Steels
Authors: Chen Xinchun , Yin Xuan , Jin Jie
Published: February 2019

To satisfy the harsh service demand of stainless steel and aviation bearing steel, the anticorrosion and wettability behaviors of 9Cr18 stainless steel and M50 bearing steel tailored by ion beam surface modification technology were experimentally investigated. By controlling the ion implantation (F+, N+, N+ + Ti+) or deposition processes, different surface-modified layers and ceramic layers or composite layers with both effects (ion implantation and deposition processes) were obtained on metal surfaces. The wettability was characterized by a contact angle instrument, and the thermodynamics stabilization of ion implantation-treated metals in corrosive solution was evaluated through an electrochemical technique. X-ray photoelectron spectroscopy (XPS) was employed for detecting the chemical bonding states of the implanted elements. The results indicated that ion implantation or deposition-induced surface-modified layers or coating layers could increase water contact angles, namely improving hydrophobicity as well as thermodynamic stabilization in corrosive medium. Meanwhile, wettability with lubricant oil was almost not changed. The implanted elements could induce the formation of new phases in the near-surface region of metals, and the wettability behaviors were closely related to the as-formed ceramic components and amorphous sublayer.
The Optimal Design of Modulation Angular Rate for MEMS-Based Rotary Semi-SINS
Authors: Zhang Jiayu , Li Jie , Che Xiaorui , Zhang Xi , Hu Chenjun , Feng Kaiqiang , Xu Tingjin
Published: February 2019

In previous studies, the semi-strapdown inertial navigation system (SSINS), based on microelectromechanical system (MEMS) sensors, had realized cross-range measurement of attitude information of high-spinning projectiles through construction of a “spin reduction” platform of the roll axis. However, further improvement of its measurement accuracy has been difficult, due to the inertial sensor error. In order to enhance the navigational accuracy, a periodically rotating method is utilized to compensate for sensor error, which is called rotation modulation. At present, the rotation scheme, as one of the core technologies, has been studied by a lot of researchers. It is known that the modulation angular rate is the main factor affecting the effectiveness of error modulation. Different from the long-endurance and low-dynamic motion characteristics of ships, however, the short-endurance and high-dynamic characteristics of the high-spinning projectile not only require the modulation angular rate to be as fast as possible but, also, the influence of the rotation speed error caused by rotating mechanism errors cannot be ignored. Combined with the rotation speed error of the rotating mechanism, this paper explored the relationship between modulation angular rate, device error, and the navigation error, and then proposed a design method for optimal modulation angular rate. Experiments were carried out to validate the performance of the method. In addition, the proposed method is applicable for rotation modulation systems with different types of motors as the rotating mechanism.
Graphene Oxide Decorated Nanometal-Poly(Anilino-Dodecylbenzene Sulfonic Acid) for Application in High Performance Supercapacitors
Authors: Dywili Nomxolisi R. , Ntziouni Afroditi , Ikpo Chinwe , Ndipingwi Miranda , Hlongwa Ntuthuko W. , Yonkeu Anne L. D. , Masikini Milua , Kordatos Konstantinos , Iwuoha Emmanuel I.
Published: February 2019

Graphene oxide (GO) decorated with silver (Ag), copper (Cu) or platinum (Pt) nanoparticles that are anchored on dodecylbenzene sulfonic acid (DBSA)-doped polyaniline (PANI) were prepared by a simple one-step method and applied as novel materials for high performance supercapacitors. High-resolution transmission electron microscopy (HRTEM) and high-resolution scanning electron microscopy (HRSEM) analyses revealed that a metal-decorated polymer matrix is embedded within the GO sheet. This caused the M/DBSA–PANI (M = Ag, Cu or Pt) particles to adsorb on the surface of the GO sheets, appearing as aggregated dark regions in the HRSEM images. The Fourier transform infrared (FTIR) spectroscopy studies revealed that GO was successfully produced and decorated with Ag, Cu or Pt nanoparticles anchored on DBSA–PANI. This was confirmed by the appearance of the GO signature epoxy C–O vibration band at 1040 cm−1 (which decreased upon the introduction of metal nanoparticle) and the PANI characteristic N–H stretching vibration band at 3144 cm−1 present only in the GO/M/DBSA–PANI systems. The composites were tested for their suitability as supercapacitor materials; and specific capacitance values of 206.4, 192.8 and 227.2 F·g−1 were determined for GO/Ag/DBSA–PANI, GO/Cu/DBSA–PANI and GO/Pt/DBSA–PANI, respectively. The GO/Pt/DBSA–PANI electrode exhibited the best specific capacitance value of the three electrodes and also had twice the specific capacitance value reported for Graphene/MnO2//ACN (113.5 F·g−1). This makes GO/Pt/DBSA–PANI a very promising organic supercapacitor material.
Root-Associated Endophytic Bacterial Community Composition of Pennisetum sinese from Four Representative Provinces in China
Authors: Deng Zhen-Shan , Zhang Bao-Cheng , Qi Xiang-Ying , Sun Zhi-Hong , He Xiao-Long , Liu Yu-Zhen , Li Jing , Chen Kai-Kai , Lin Zhan-Xi
Published: February 2019

Pennisetum sinese, a source of bio-energy with high biomass production, is a species that contains high crude protein and will be useful for solving the shortage of forage grass after the implementation of “Green for Grain” project in the Loess plateau of Northern Shaanxi in 1999. Plants may receive benefits from endophytic bacteria, such as the enhancement of plant growth or the reduction of plant stress. However, the composition of the endophytic bacterial community associated with the roots of P. sinese is poorly elucidated. In this study, P. sinese from five different samples (Shaanxi province, SX; Fujian province, FJ; the Xinjiang Uyghur autonomous prefecture, XJ and Inner Mongolia, including sand (NS) and saline-alkali land (NY), China) were investigated by high-throughput next-generation sequencing of the 16S rDNA V3-V4 hypervariable region of endophytic bacteria. A total of 313,044 effective sequences were obtained by sequencing five different samples, and 957 effective operational taxonomic units (OTUs) were yielded at 97% identity. The phylum Proteobacteria, the classes Gammaproteobacteria and Alphaproteobacteria, and the genera Pantoea, Pseudomonas, Burkholderia, Arthrobacter, Psychrobacter, and Neokomagataea were significantly dominant in the five samples. In addition, our results demonstrated that the Shaanxi province (SX) sample had the highest Shannon index values (3.795). We found that the SX (308.097) and NS (126.240) samples had the highest and lowest Chao1 richness estimator (Chao1) values, respectively. Venn graphs indicated that the five samples shared 39 common OTUs. Moreover, according to results of the canonical correlation analysis (CCA), soil total carbon, total nitrogen, effective phosphorus, and pH were the major contributing factors to the difference in the overall composition of the bacteria community in this study. Our data provide insights into the endophytic bacteria community composition and structure of roots associated with P. sinese. These results might be useful for growth promotion in different samples, and some of the strains may have the potential to improve plant production in future studies.
Origin of Smectite in Salinized Soil of Junggar Basin in Xinjiang of China
Authors: Xie Haixia , He Shuai , Huang Chuanqin , Tan Wenfeng
Published: February 2019

In this paper, salinized soils with different degrees of salinity are sampled in Junggar Basin of Xinjiang of China. The X-ray diffraction, transmission electron microscopy, and inductively coupled plasma mass spectrometry are employed to investigate the morphology and distribution characteristics of smectite in salinized soil profiles. In the salinized soil profiles of this region, crystals of smectite are poor where lattice fringes are not parallel. In all soil layers, the content of smectite in the soil increases with the decrease in content of illite, which has demonstrated significant negative correlation (r = 0.79, n = 50, p < 0.01) between illite and smectite. This phenomenon has demonstrated that illite may be transformed into smectite in salinized soils of studied regions. In general, the transformation process of illite to smectite is affected by climate condition. The δ18O values of secondary carbonate in the 0–10 cm soil layers is higher than that in deep soil layers, which indicates that δ18O concentrates in surface soil and reflects temperature rise during soil layer formation. The δ13C values of secondary carbonate and soil organic matter in 0–10 cm soil layers are higher than that in deep soil layers. It indicates that C4 plants were the main plants, which reflects that the climate was relatively dry during the formation of the surface soil. Thus, the climate during the surface soil formation is arid, which is not conducive for leaching K+ from illite of the 0–10 cm soil to form smectite. As a result, the content of the smectite becomes lowest in the soil surface. In the relative humid condition of deep soil layers, the K+ of the illite of the soil would be relative easily leached and more smectite may be formed. Furthermore, the presence of salt in the salinized soil would promote the formation of smectite in Junggar Basin of Xinjiang. A lot of Ca2+, Na+ and Mg2+ in the soil solution of salinized soils would enter into the illite and occupy K+ positions. The studied result shows that the amount of smectite would increase with the increase of salt below 10 cm of the soil layer, where the amount of smectite would be significantly correlated with soil electrical conductivity (r = 0.64, n = 39, p < 0.01). In the Junggar Basin in Xinjiang, therefore, the salinized soil below 10 cm would have the necessary water conditions and chemical components for illite transformation to smectite.
Molecular Simulation of the Adsorption and Diffusion in Cylindrical Nanopores: Effect of Shape and Fluid–Solid Interactions
Authors: Cárdenas Harry , Müller Erich A.
Published: February 2019

We report on molecular simulations of model fluids composed of three tangentially bonded Lennard-Jones interaction sites with three distinct morphologies: a flexible “pearl-necklace” chain, a rigid “stiff” linear configuration, and an equilateral rigid triangular ring. The adsorption of these three models in cylindrical pores of diameters 1, 2, and 3 nm and with varying solid–fluid strength was determined by direct molecular dynamics simulations, where a sample pore was placed in contact with a bulk fluid. Adsorption isotherms of Type I, V, and H1 were obtained depending on the choice of pore size and solid–fluid strength. Additionally, the bulk-phase equilibria, the nematic order parameter of the adsorbed phase, and the self-diffusion coefficient in the direction of the pore axis were examined. It was found that both the molecular shape and the surface attractions play a decisive role in the shape of the adsorption isotherm. In general, the ring molecules showed a larger adsorption, while the fully flexible model showed the smallest adsorption. Morphology and surface strength were found to have a lesser effect on the diffusion of the molecules. An exceptional high adsorption and diffusion, suggesting an enhanced permeability, was observed for the linear stiff molecules in ultraconfinement, which was ascribed to a phase transition of the adsorbed fluid into a nematic liquid crystal.
Transport and Optical Gaps in Amorphous Organic Molecular Materials
Authors: San-Fabián Emilio , Louis Enrique , Díaz-García María A. , Chiappe Guillermo , Vergés José A.
Published: February 2019

The standard procedure to identify the hole- or electron-acceptor character of amorphous organic materials used in OLEDs is to look at the values of a pair of basic parameters, namely, the ionization potential (IP) and the electron affinity (EA). Recently, using published experimental data, the present authors showed that only IP matters, i.e., materials with IP > 5.7 (<5.7) showing electron (hole) acceptor character. Only three materials fail to obey this rule. This work reports ab initio calculations of IP and EA of those materials plus two materials that behave according to that rule, following a route which describes the organic material by means of a single molecule embedded in a polarizable continuum medium (PCM) characterized by a dielectric constant ε . PCM allows to approximately describe the extended character of the system. This “compound” system was treated within density functional theory (DFT) using several combinations of the functional/basis set. In the preset work ε was derived by assuming Koopmans’ theorem to hold. Optimal ε values are in the range 4.4–5.0, close to what is expected for this material family. It was assumed that the optical gap corresponds to the excited state with a large oscillator strength among those with the lowest energies, calculated with time-dependent DFT. Calculated exciton energies were in the range 0.76–1.06 eV, and optical gaps varied from 3.37 up to 4.50 eV. The results are compared with experimental data.
Systematic Identification of Thiosemicarbazides for Inhibition of Toxoplasma gondii Growth In Vitro
Authors: Paneth Agata , Węglińska Lidia , Bekier Adrian , Stefaniszyn Edyta , Wujec Monika , Trotsko Nazar , Dzitko Katarzyna
Published: February 2019

One of the key stages in the development of new therapies in the treatment of toxoplasmosis is the identification of new non-toxic small molecules with high specificity to Toxoplasma gondii. In the search for such structures, thiosemicarbazide-based compounds have emerged as a novel and promising leads. Here, a series of imidazole-thiosemicarbazides with suitable properties for CNS penetration was evaluated to determine the structural requirements needed for potent anti-Toxoplasma gondii activity. The best 4-arylthiosemicarbazides 3 and 4 showed much higher potency when compared to sulfadiazine at concentrations that are non-toxic to the host cells, indicating a high selectivity of their anti-toxoplasma activity.
Sampling for DUS Test of Flower Colors of Ranunculus asiaticus L. in View of Spatial and Temporal Changes of Flower Colorations, Anthocyanin Contents, and Gene Expression Levels
Authors: Liu Yanfang , Lin Tao , Du Lijuan , Wang Jiangmin , Yang Xiaohong , Zhang Jianhua , Zhang Peng , Li Yangang , Shi Junfeng , Yang Xuhong
Published: February 2019

Sampling for DUS test of flower colors should be fixed at the stages and sites that petals are fully colored, and besides, flower colorations are uniform among individuals and stable for a period of time to allow testers to get consistent results. It remains a problem since spatial and temporal flower colorations are reported a lot but their change traits are little discussed. In this study, expression state, uniformity and stability of color phenotypes, anthocyanin contents, and gene expression levels were taken into account based on measurements at 12 development stages and three layers (inner, middle, and outer petals) of two varieties of Ranunculus asiaticus L. to get their best sampling. Our results showed that, outer petals of L9–L10 (stage 9–stage 10 of variety ‘Jiaoyan zhuanhong’) and C5–C6 (stage 5–stage 6 of variety ‘Jiaoyan yanghong’) were the best sampling, respectively. For DUS test, it is suggested to track flower colorations continuously to get the best sampling as well as representative colors since different cultivars had different change traits, and moreover, full expression of color phenotypes came later and lasted for a shorter duration than those of anthocyanin contents and gene expressions. Our innovation exists in following two points. Firstly, a model of change dynamic was introduced to illustrate the change traits of flower colorations, anthocyanin contents, and gene expressions. Secondly, genes used for expression analysis were screened on account of tentative anthocyanins, which were identified based on comparison between liquid chromatography–mass spectrometry (LC–MS) results and molecular mass and mass fragment pattern (M2) of each putative anthocyanin and their fragments deduced in our previous study. Gene screening in this regard may also be interest for other non-model plant genera with little molecular background.
Gastroprotection of Calein D against Ethanol-Induced Gastric Lesions in Mice: Role of Prostaglandins, Nitric Oxide and Sulfhydryls
Authors: Sánchez-Mendoza María Elena , López-Lorenzo Yaraset , Cruz-Antonio Leticia , Matus-Meza Audifás-Salvador , Sánchez-Mendoza Yolanda , Arrieta Jesús
Published: February 2019

Peptic ulcers are currently treated with various drugs, all having serious side effects. The aim of this study was to evaluate the gastroprotective activity of calein D (from Calea urticifolia), a sesquiterpene lactone with a germacrane skeleton. Gastric lesions were induced in mice by administering ethanol (0.2 mL) after oral treatment with calein D at 3, 10 and 30 mg/kg, resulting in 13.15 ± 3.44%, 77.65 ± 7.38% and 95.76 ± 2.18% gastroprotection, respectively, to be compared with that of the control group. The effect found for 30 mg/kg of calein D was not reversed by pretreatment with NG-nitro-l-arginine methyl ester (l-NAME, 70 mg/kg, ip), indomethacin (10 mg/kg, sc) or N-ethylmaleimide (NEM, 10 mg/kg, sc). Hence, the mechanism of action of calein D does not involve NO, prostaglandins or sulfhydryl compounds. Calein D was more potent than carbenoxolone, the reference drug. The findings for the latter are in agreement with previous reports.
Simultaneous Determination of Pharmaceuticals by Solid-phase Extraction and Liquid Chromatography-Tandem Mass Spectrometry: A Case Study from Sharjah Sewage Treatment Plant
Authors: Semreen Mohammad H. , Shanableh Abdallah , Semerjian Lucy , Alniss Hasan , Mousa Mouath , Bai Xuelian , Acharya Kumud
Published: February 2019

The present work describes the optimization and validation of a highly selective and sensitive analytical method using solid phase extraction and liquid chromatography tandem mass spectrometry (SPE LC-MS/MS) for the determination of some frequently prescribed pharmaceuticals in urban wastewater received and treated by Sharjah sewage treatment plant (STP). The extraction efficiency of different SPE cartridges was tested and the simultaneous extraction of pharmaceuticals was successfully accomplished using hydrophilic-lipophilic-balanced reversed phase Waters® Oasis HLB cartridge (200 mg/ 6 mL) at pH 3. The analytes were separated on an Aquity BEH C18 column (1.7 µm, 2.1 mm × 150 mm) using gradient elution and mass spectrometric analysis were performed in multiple reactions monitoring (MRM) selecting two precursor ions to produce ion transition for each pharmaceutical using positive electrospray ionization (+ESI) mode. The correlation coefficient values in the linear calibration plot for each target compound exceeded 0.99 and the recovery percentages of the investigated pharmaceuticals were more than 84%. Limit of detection (LOD) varied between 0.1–1.5 ng/L and limit of quantification (LOQ) was 0.3–5 ng/L for all analytes. The precision of the method was calculated as the relative standard deviation (RSD%) of replicate measurements and was found to be in the ranges of 2.2% to 7.7% and 2.2% to 8.6% for inter and intra-day analysis, respectively. All of the obtained validation parameters satisfied the requirements and guidelines of analytical method validation.
Electrical Properties and Electromagnetic Interference Shielding Effectiveness of Interlayered Systems Composed by Carbon Nanotube Filled Carbon Nanofiber Mats and Polymer Composites
Authors: Ramírez-Herrera Claudia Angélica , Gonzalez Homero , de la Torre Felipe , Benitez Laura , Cabañas-Moreno José Gerardo , Lozano Karen
Published: February 2019

The demand for multifunctional requirements in aerospace, military, automobile, sports, and energy applications has encouraged the investigation of new composite materials. This study focuses on the development of multiwall carbon nanotube (MWCNT) filled polypropylene composites and carbon nanofiber composite mats. The developed systems were then used to prepare interlayered composites that exhibited improved electrical conductivity and electromagnetic interference (EMI) shielding efficiency. MWCNT-carbon nanofiber composite mats were developed by centrifugally spinning mixtures of MWCNT suspended in aqueous poly(vinyl alcohol) solutions. The developed nanofibers were then dehydrated under sulfuric acid vapors and then heat treated. Interlayered samples were fabricated using a nanoreinforced polypropylene composite as a matrix and then filled with carbon fiber composite mats. The in-plane and through-plane electrical conductivity of an eight-layered flexible carbon composite (0.65 mm thick) were shown to be 6.1 and 3.0 × 10−2 S·cm−1, respectively. The EMI shielding effectiveness at 900 MHz increased from 17 dB for the one-layered composite to 52 dB for the eight-layered composite. It was found that the reflection of the electromagnetic waves was the dominating mechanism for EMI shielding in the developed materials. This study opens up new opportunities for the fabrication of novel lightweight materials that are to be used in communication systems.
Preparation of Tin Oxide Quantum Dots in Aqueous Solution and Applications in Semiconductor Gas Sensors
Authors: Liu Jianqiao , Xue Weiting , Jin Guohua , Zhai Zhaoxia , Lv Jiarong , Hong Wusong , Chen Yuzhen
Published: February 2019

Tin oxide quantum dots (QDs) were prepared in aqueous solution from the precursor of tin dichloride via a simple process of hydrolysis and oxidation. The average grain size of QDs was 1.9 nm. The hydrothermal treatment was used to control the average grain size, which increased to 2.7 and 4.0 nm when the operating temperatures of 125 and 225 °C were employed, respectively. The X-ray photoelectron spectroscopy (XPS) spectrum and X-ray diffraction analysis (XRD) pattern confirmed a rutile SnO2 system for the QDs. A band gap of 3.66 eV was evaluated from the UV-VIS absorption spectrum. A fluorescence emission peak was observed at a wavelength of 300 nm, and the response was quenched by the high concentration of QDs in the aqueous solution. The current-voltage (I-V) correlation inferred that grain boundaries had the electrical characteristics of the Schottky barrier. The response of the QD thin film to H2 gas revealed its potential application in semiconductor gas sensors.
Personalized Nutrition Approach in Food Allergy: Is It Prime Time Yet?
Authors: D’Auria Enza , Abrahams Mariette , Zuccotti Gian Vincenzo , Venter Carina
Published: February 2019

The prevalence of food allergy appears to be steadily increasing in infants and young children. One of the major challenges of modern clinical nutrition is the implementation of individualized nutritional recommendations. The management of food allergy (FA) has seen major changes in recent years. While strict allergen avoidance is still the key treatment principle, it is increasingly clear that the avoidance diet should be tailored according to the patient FA phenotype. Furthermore, new insights into the gut microbiome and immune system explain the rising interest in tolerance induction and immunomodulation by microbiota-targeted dietary intervention. This review article focuses on the nutritional management of IgE mediated food allergy, mainly focusing on different aspects of the avoidance diet. A personalized approach to managing the food allergic individual is becoming more feasible as we are learning more about diagnostic modalities and allergic phenotypes. However, some unmet needs should be addressed to fully attain this goal.