bluuebunny/crossref_metadata_2025 huggingface dataset:
A review of intelligent methods of health assessment technology
Authors: Liu Diyi , Peng Linyuan , Zhao Zhiyao
Published: August 2023

The core technology of prognostics and health management, a key technology that detects system anomalies, is health assessment, which analyzes and diagnoses the current system working status and quantitatively assesses the health of the system. This paper reviews the development of health assessment technology in recent years from three aspects: health definition, health assessment indicators, and health assessment approaches. In terms of health definition, this paper summarizes three common definition methods. Health assessment indicators are reviewed from four levels: process variables, data features, residuals, and fusion indicators. Finally, health assessment approaches are divided into model-based, data-driven, and fusion approaches. Concerning the data-driven approach, rapidly developing health assessment research based on an intelligent approach is discussed. The paper also compares various approaches and identifies the current challenges and development prospects of this technology.
Adolescent assent in research: a relational ethics approach
Authors: Hapsari Happy Indri , Huang Mei-Chih
Published: August 2023

Researchers often neglect adolescents’ willingness to participate in research. The granting of permission by parents is sometimes not in accordance with the unwillingness of adolescents. Relational ethics is the right approach to overcome inconsistencies between legal and ethical agreements in granting parental permission and adolescent’s assent. This is because relational ethics is based on building relationships among many parties. The focus of this case study is to improve understanding of the assent of adolescents through intensive study of research conflict, reinforced using existing research and to understand how relational ethics can be used as an approach in decision-making, especially in conflicts between parental permission and assent from adolescents.
Nomophobia Sebagai Determinan Kualitas Tidur Mahasiswa Keperawatan
Authors: Riandi Irnanda Naufal , Mulyono Wastu Adi , Choiruna Hasby Pri
Published: August 2023

Background. Sleep is a basic human need that has to be fulfilled as a fundamental of physiological part, but mostly need of sleep not fulfil enough and have a bad sleep quality. There are numerous factors that affect someone sleep quality, those are age, gadget, nomophobia, and physical activity. This research purposed to predict the most influential variable of sleep quality among nursing students.

Methods. This cross-sectional study aimed to predict the influential variable that affect sleep quality among nursing students. Regression analysis to 80 respondents that selected by simple random sampling from students batch 2019, 2020, 2021, and 2022 had done to examine the determinant of sleep quality nursing students.

Results. From four predictors that used in this research, only nomophobia (p=0,001) which had a significant correlation to sleep quality by partial t  test. Simultaneous F test indicated that all  variable were significantly correlated with sleep quality (p=0,004) and coefficient determination 18,1% . Simple linear regression with nomophobia and sleep quality get significant result (p=0,001) and coefficient determination 0,171.

Conclusion. Nomophobia is a determinant variable of sleep quality among nursing academic community. Sleep problem among nursing students around a fifth affected by nomophobia. It is suggested to nursing student aware of their anxiety to gadget because it might help for better sleep quality.
Pengelolaan Sumber Daya Posyandu dalam Pemantauan Pertumbuhan Balita: Studi Kualitatif
Authors: Rahmawati Lina , Rahfiludin Muhammad Zen , Kartasurya Martha Irene
Published: August 2023

Background. Various problems were faced by midwives at the posyandu to monitor the growth of toddlers. These problems include anthropometric measurements sometimes needing to be carried out correctly, lack of standardized anthropometric tools, lack of budget for Supplementary Feeding (PMT) for toddlers, not running the 5 tables system, and low visits to the posyandu.   

Objectives: This study aims to describe the barriers and solutions in managing posyandu resources by midwives to optimize the monitoring of the growth and development of toddlers.

Methods. The type of research is qualitative with a case study design. Data were collected through in-depth interviews and observations from September to December 2022 at 4 health centers in Tegal Regency, with the prevalence of stunted toddlers in low (2 health centers) and high (2 health centers). In-depth interviews were conducted with 4 village midwives (primary informants) and 3 coordinating midwives (triangulation informants). Observations were conducted at 4 posyandu in the puskesmas working areas. This analysis used a thematic approach.

Results. The study results showed a lack of cadres’ ability in taking anthropometric measurements correctly, the fulfilment of the posyandu budget depending on village government policies, and the distance of the posyandu. Efforts are conducting routine cadre coaching and incentives, advocacy to village government and cross-sector, and shadow posyandu.

Conclusion. The study concludes that the obstacles to posyandu management can be overcome by strengthening cross-sectoral cooperation.
Faktor-faktor yang Berhubungan dengan Dukungan Sosial pada Mahasiswa Profesi Ners di masa pandemi COVID-19
Authors: Sanger Ailine Yoan , Lainsamputty Ferdy , Yacobus Geby Sabatina
Published: August 2023

Background: Heavy burden were experienced by professional nursing students such as academic, financial, and psychological problems. Adequate social support is needed, especially during clinical practice in time of the COVID-19 pandemic.
Objective: The study aimed to examine the relationship between sociodemographic factors and social support of professional nursing students during the pandemic.
Method: The study design was a descriptive correlational and cross-sectional with 102 samples, recruited using purposive sampling technique from a nursing campus in North Sulawesi. The questionnaire used was the Multidimensional Scale of Perceived Social Support (MSPSS). Independent T-Test, One-Way ANOVA, and Pearson correlation were performed to test the relationship between variables.
Results: Social support had significant differences with gender, place of residence, relationship status, religion, and knowledge of COVID-19 (p<0.05).
Conclusion: Respondents who received higher social support were female students, living outside their own boarding house or home, having spouse, and having good knowledge of COVID-19.
The Relationship Between Eating Behavior and Diseases Experienced by Malay Families in Medan
Authors: Nasution Siti Zahara , Amal Muhammad Ridha Haykal , Matondang Ika Maidah Nazli
Published: August 2023

ABSTRACT

Latar Belakang. Perilaku makan merupakan cara seseorang atau sekelompok orang untuk memilih makanan dan mengonsumsinya. Perilaku makan tidak sehat akan berdampak pada status kesehatan. Setiap daerah di Indonesia memiliki kebiasaan makan yang berbeda-beda. Perilaku dan kebiasaan makan dapat digolongkan sebagai cerminan budaya dan ciri khas dari daerah tersebut. Suku melayu dikenal oleh masyarakat luas dengan berbagai keanekaragamannya seperti budaya, kesenian, sejarah hingga makanan khas yang disajikan. Penelitian ini bertujuan untuk melihat hubungan antara perilaku makan dan penyakit yang dialami oleh keluarga Melayu di Medan.

Metode. Penelitian ini bersifat kuantitatif dengan pendekatan deskriptif korelasional. Penelitian in dilakukan di Medan. Populasi dalam penelitian ini adalah keluarga Melayu yang tinggal di Medan. Sampel penelitian sebanyak 177 orang dengan menggunakan teknik purposive sampling. Instrumen penelitian menggunakan kuesioner dan analisis data yang digunakan adalah Spearman Rho.

Hasil. Hasil penelitian menunjukkan 148 responden (83,6%) memiliki perilaku makan yang kurang baik dan 29 responden (16,4%) memiliki perilaku makan yang baik. Sebanyak 145 responden (81,9%) memiliki riwayat penyakit berupa obesitas, hipertensi, gastritis, diabetes melitus, dan radang sendi, sedangkan 32 responden (18,1%) tidak memiliki riwayat penyakit. Hasil uji statistik Spearman rho didapatkan angka signifikan atau angka p = 0,000 < (0,05).

Kesimpulan. Terdapat hubungan antara perilaku makan dengan penyakit yang dialami keluarga Melayu Medan. Mengonsumsi makanan yang mengandung lemak dan garam secara berlebihan dapat menyebabkan penyakit hipertensi. Jenis makanan asam, pedas, makanan yang berlemak dan mengandung gas dapat merangsang peningkatan asam lambug yang menyebabkan penyakit gastritis. Kebiasaan makan berlebihan serta kebiasaan mengemil makanan ringan menyebabkan seseorang mengalami kelebihan berat badan.

Keywords: Keluarga Suku Melayu, Kebiasaan Makan, Penyakit yang dialami
Nurses’ Perceived Barriers to The Prevention of Pressure Injury and Related Factors in Indonesia
Authors: Sari Yunita , Upoyo Arif Setyo , Sumeru Annas , Mulyono Wastu Adi , Anam Akhyarul
Published: August 2023

Background. Incidence of pressure injury (PI) in Indonesia is high. Therefore, Indonesian nurses should take measures to prevent PI from developing. Considering the high incidence of PI in Indonesia, there could be some barriers faced by Indonesian nurses in performing prevention of PI. However, to date, no study has assessed the perceived barriers and factors associated with perceived barrier of prevention of PI in Indonesian nurses. Therefore, this study aimed to assess perceived barriers to prevention of PI and associated factors in Indonesian nurses.

Methods. A total of 521 nurses participated in the study. A pressure injury prevention barriers questionnaire was used to assess perceived barriers of prevention of PI. A multivariate logistic regression model was used to analyse factors associated with the perceived barriers.

 Results. The two most commonly perceived barriers in nurses were lack of preventive devices such as special mattresses, cushions, and skin care products, and a lack of training courses related to prevention of PI. Associated factors emerging from the results were working experience (AOR =1.74) and working unit (AOR =2.73).
Qualitative Test of Rhodamine B and Methanyl Yellow Using the Spot test Method on Packaged Beverage at The Center for Drug and Food Control Denpasar
Authors: Simamora Yola Monalisa R , Puspawati Gusti Ayu Kadek Diah
Published: August 2023

Rhodamine B and Methanyl Yellow are synthetic dyes that are widely used in the textile and paper industries. Rhodamine B is a synthetic dye that produces a red color and Methanyl Yellow is a synthetic dye that produces a yellow color. Based on the decision of the Ministry of Health (Permenkes) No. 239/Menkes/Per/V/85 Rhodamin B and Methanyl Yellow are designated as substances whose use is prohibited in food products. The use of this dye if consumed can cause irritation to the respiratory tract, skin, eyes, digestive tract, the danger of liver cancer, and as a tumor-triggering agent. So based on the above problems it is necessary to identify Rhodamin B and Methanyl Yellow in food products. The method that has been developed and used in the identification of Rhodamine B and Methanyl Yellow is using a qualitative descriptive analysis technique with the spot test method. This research was conducted by direct observation to the field to find indications for the use of Rhodamine B and Methanol Yellow to be tested. This study aims to determine whether or not the use of Rhodamine B and Methanyl Yellow in packaged beverage products at the Denpasar Food and Drug Supervisory Agency. The research samples were 2 packaged drinks which had striking red and striking yellow colors. The results showed that none of the samples contained Rhodamine B and Methanol Yellow synthetic dyes.
Exposure of Covid-19 Vaccination Information and Willingness to Receive Vaccination
Authors: Pradani Firda Yanuar , Fuadiyah M. Ezza Azmi , Widawati Mutiara
Published: August 2023

One of the strategies to curb the Covid-19 spread was administering the Covid-19 vaccine as it remains the most effective means to achieve control of the pandemic. As mandated by Minister of Health Decree Number 10/2021, Pangandaran District has to conduct Covid-19 vaccination to 341.216 individuals as the target population. Per July 26th, the Covid-19 vaccination coverage is still below 10% of target with Parigi, Sidamulih, and Mangunjaya Subdistricts as subdistricts with the lowest vaccination coverage. Vaccine hesitancy and misinformation present major barriers to achieving community coverage and immunity. This was a cross-sectional rapid survey conducted in August 2021, held in subdistrict Parigi, Sidamulih, and Mangunjaya of Pangandaran District, West Java Province. There was a total of 915 samples participated through an electronic questionnaire. Data were analyzed as univariate, and chi-square was used in bivariate analysis. There’s a significant relationship between the exposure of information about the Covid-19 vaccine with willingness to receive the vaccination
RISK FACTORS OF ACUTE OTITIS MEDIA IN PRIMARY SCHOOL CHILDREN IN PURWOKERTO
Authors: Nurmalasari Intan
Published: August 2023

Background: Acute Otitis Media (OMA) or commonly known by the public as congek, is a condition where there is fluid in the middle ear with signs and symptoms of infection, and can be caused by various pathogens. AOM that gets the right treatment can interfere with daily activities and have an impact on permanent hearing loss. One of the efforts to prevent and overcome AOM cases is to know the risk factors. The purpose of this research is to find out what risk factors can cause AOM in elementary school children

Methods: This study uses a quantitative method with a Case-Control approach and the sampling technique in the case group using Total Sampling and in the control group using Purposive Sampling. Respondents in this study were parents of elementary school students who were sampled in this study at SDN 1 Bobosan and SDN 4 Kranji with a total of 34 respondents, while the control group in this study used a ratio of 1:2. The variables used in this study were gender, immunization status, history of allergies, history of ARI, history of exclusive breastfeeding, parental income, number of family members in the house, exposure to cigarette smoke. Data collection was done by interview. Data were analyzed using Chi-Square test and continued with logistic regression test. The risk factor for AOM is known by calculating the odds ratio (OR) value.

Results: Bivariate results showed a relationship between history of exclusive breastfeeding, parental income, and exposure to cigarette smoke with the incidence of AOM in elementary school children in Purwokerto. Multivariate results showed that there was an influence between cigarette smoke exposure (OR 7,043) and the incidence of AOM in elementary school children in Purwokerto

Conclusions: Exposure to cigarette smoke is the most influential factor in the incidence of AOM in primary school children.
Prevalence of Pediculosis Capitis in Indonesia
Authors: Trasia Reqgi First
Published: August 2023

Head lice infestation is still a health problem in Indonesia. Currently, there has been a lot of research on the incidence of pediculosis capitis. However, there are still few articles that systematically review the incidence of head lice infestation. The purpose of writing this article is to examine cases of pediculosis capitis in several regions in Indonesia in order to obtain systematic and comprehensive prevalence data. A literature search was carried out in October 2021 through Google Scholar and obtained 13 relevant articles. Based on the above review, it can be concluded that the highest incidence of pediculosis capitis is in Islamic boarding schools, attacking elementary school-aged children, with a prevalence range of 29.3%-88.9% and can occur throughout Indonesia. This condition can be caused by a lack of personal hygiene, so that it can continue to be a source of infestation for children around it.
Investigation of The Presence of Aedes aegypti Larvae and Its Related Factors in Air Putih Community Health Center, Samarinda
Authors: Newyearsi Sri evi , Silviana Silviana
Published: August 2023

Dengue Hemorrhagic Fever (DHF) is a public health problem in Indonesia until now because there are still many morbidity and mortality rates every year. DHF cases in the working area of the Air Putih Health Center fluctuate every year, in 2019 there were 109 cases, in 2020 there were 29 cases and in 2021 there were 44 cases. The purpose of this study was to determine the relationship between breeding places and PSN behavior with the presence of Aedes aegypti mosquito larvae in the working area of the Air Putih Public Health Center, Samarinda City. This study used quantitative, analytic survey methods with a cross-sectional approach where the subjects in this study were houses within the working area of the Air Putih Community Health Center with a sample of 59 houses, a sampling technique set by WHO. Collecting data with a questionnaire, check list and analyzed using the fisher's exact test. As for the bivariate test results, it was found that there was a relationship between TPA for daily needs (p-value = 0.00), TPA not for daily needs (p-value = 0.00) and PSN behavior (p-value = 0.05 ) with the presence of Aedes aegypti mosquito larvae in the working area of the Air Putih Public Health Center, Samarinda City. Suggestions in this study, the people in the working area of the Air Putih Health Center can reduce mosquito breeding places, drain the TPA regularly and bury used items that are not used. It is also hoped that the Puskesmas can improve coordination with the community and cadres in checking mosquito larvae regularly so that they can eradicate the DHF vector.
Analysis of Hypertension-Related Factors in Rural Areas, Banyumas Regency
Authors: Nafisah Lu'lu , Anindita Asti Nadia
Published: August 2023

Background: Hypertension is a public health concern since it has the potential to cause consequences such as stroke, coronary heart disease, renal failure, and eventually become one of the leading causes of premature death worldwide. In many parts of Indonesia, notably rural regions, the prevalence of hypertension is relatively high. Information on hypertension risk factors in rural regions is required to develop effective interventions for the prevention and treatment of hypertension among rural communities. The purpose of this study is to investigate the risk factors associated with the prevalence of hypertension in rural areas.

Methods: This research is a quantitative study using a case control approach with a total sample of 76 respondents. The comparison of the case and control groups in this study was 1:1.Questionnaires are used as instruments in data collection. The chi square test was used to assess the data.

Results: Most of the respondents were in the late elderly age (35.5%), female (84.2%), had a family history of hypertension (40.8%), had normal BMI (30.3%), and obesity (28.9%). Most of the respondents did not smoke (93.4%), consumed less vegetables and fruit (78.9%), were not stressed (93.4%) and had sufficient levels of physical activity (82.9%).The findings revealed there was a relationship between age and hypertension (p value = 0.000). Gender (p = 0.753), family history of hypertension (p = 1,000), obesity (p = 0.502), smoking habit (p = 1,000), fruit and vegetable consumption (p = 0.778), level of stress (p = 1000), and physical activity (p = 0.542) were not linked with hypertension.

Conclusions: Community health activities in rural regions must be strengthened in order to monitor public health status (including blood pressure monitoring) and approach the community to increase their involvement in these activities.
A Study on Tense Errors in the Compositions of Secondary Students in Brunei
Authors: Daud Hazirah
Published: August 2023

This study examines the errors in tenses made by 34 secondary students in Brunei who were asked to write a short story based on a picture composition, with the focus on testing Krashen's Natural Order Hypothesis (NOH) in L2 acquisition. The objective of the study is to test the theory in NOH that irregular past tense is acquired before regular past tense through the analysis of the errors made in the verbs. The collected data were analyzed based on the four steps of error analysis: identification, description, explanation, and evaluation. The findings suggest that irregular past tense for verbs such as "go," "is," "eat," and "buy" were the most commonly found errors, mainly due to inappropriate or incorrect tense markings. Contrary to the hypothesis, the study suggests that irregular verbs are not necessarily acquired before regular past tense, possibly due to the large number of irregular verbs that learners need to know. Thus, the article concludes that the study provides useful information for language pedagogy and emphasizes the importance of considering the learners' L1 and individual differences in language acquisition.
The Benefits of Interactive Media Websites Through Google Sites on Learning Outcomes of Elementary School Students
Authors: Halimatusyadiah Hema , Disman Disman
Published: August 2023

The application of technology in education will improve teachers’ ability to convey material to students, make it easier for students to understand the material presented, and make time more effective and efficient. Advances in computers are also closely related to the world of education. Most schools also include computers as a compulsory subject. Technology's role in education is growing rapidly, and the need for technology is indispensable in the current era of globalization. By utilizing recent technological developments, the aims of this research are (1) to develop website-based learning media; (2) to analyze the differences in learning outcomes between students who use websites and conventional media. Researchers use a Google Sites application to support the development of web-based learning media. Teaching staff can use Google Sites to carry out various learning activities. The stages of this research method are experimental research with a posttest only control group design. The subjects of this study were students of class IV A and B at SDN 2 Karangsari—intake of research class through random sampling technique. Activities in data analysis include data processing and statistical analysis. The conclusion of this study is that the learning model through website learning media can generally motivate students to improve learning outcomes. Students gave a positive response to the implementation of the website learning model.
Analysis of Petroleum Products Spill Potential Impact (Gasoline and Diesel Fuels) on Soil Fertility Quality Around Pertamina Rewulu-Cilacap Pipeline Bantul-Yogyakarta
Authors: Muthohharoh Thaifah , Latumahina Linda Johana , Suyanta Suyanta , Suherman Suherman
Published: August 2023

This research focused on analyzing the potential impact of petroleum products spill (gasoline and diesel fuels) on soil fertility quality around PERTAMINA Rewulu-Cilacap Pipeline Bantul, Yogyakarta. The objectives of this study were to analyze the change of soil fertility parameters that are contaminated by gasoline and diesel fuel oils, compared to uncontaminated soil as control. Soil samples were collected from Pertamina Rewulu-Cilacap around the pipeline. Some physico-chemical properties that reflect soil fertility were determined using standard physico-chemical methods and Total Petroleum Hydrocarbon (TPH) using Gas Chromatography. Soil samples were divided into three points (I, II, and III) based on taken places. The study started with analysis of physical and chemical properties of the soil, then petroleum products (gasoline and diesel) were added to the soil samples. The contaminated soil and control then analyzed for identify the fertility quality and TPH content. The results showed that petroleum product contamination increases N, P, and reduces K concentrations in the soil. The sample in point III had the highest values of TPH concentration without any additional petroleum products. After addition of a dose of 13% gasoline, the concentration of TPH in point I, II, and III increased by 0.036%, 0.08% and 0.008%. With the addition of 15% diesel in the sample, the TPH concentration of point I, II, and III increased by 0.892 %, 1.198%, and 0.91%.
Synthesis, Characterization, and Antibacterial Activity Test of Geothermal Silica/AgNO3 Thin Film
Authors: Astuti Yayuk , Rahayu R.A. Yunita Suci , Arnelli Arnelli
Published: August 2023

Geothermal silica waste offers convenient, economical, and environmentally friendly material with high hydrophobicity to produce thin films. Silica-thin films from geothermal waste using the sol-gel method, though, no addition of AgNO3 was conducted for antibacterial functions. This study aims to produce silica-thin films from geothermal waste with the addition of AgNO3 and analyze the antibacterial activity. The procedures carried out in this research were (i) an acid leaching process using HNO3; (ii) the production of silica thin film with and without the addition of AgNO3; (iii) thin film characterization including a water contact angle measurement (WCA), XRF, FTIR, XRD and SEM-EDX on silica thin film samples with and without the addition of AgNO3; and (iv) antibacterial activity test. The results show the optimum HNO3 concentration for the acid leaching process was 20%, yielding 99.08% SiO2 by mass. The WCA of the silica thin film in the presence and absence of AgNO3 reached a value of ±160°, indicating the addition of AgNO3 did not decrease the contact angle of the silica thin film. This research employed smart deconvolution of IR Spectra using Fityk software which reveals a higher area ratio for Si-O-Si relative to Si-OH. Furthermore, it was observed that the silica thin films exhibited an amorphous morphology, both without and with the addition of AgNO3, with Ag discovered to be dispersed on the thin film. However, despite the presence of Ag, both TF20 and TF20+Ag samples were found to be ineffective in inhibiting bacterial growth, as evidenced by bacteria-free zones on the samples.
Synthesis and Evaluation of Some Sulfonamide-Substituted of 1,3,5-Triphenyl Pyrazoline Derivatives as Tyrosinase Enzyme Inhibitors
Authors: Herfindo Nofal , Frimayanti Neni , Ikhtiarudin Ihsan , Eryanti Yum , Zamri Adel
Published: August 2023

Pyrazoline is well-known as heterocycles compound that can exhibit many biological effects. In this work, we synthesized a series of sulfonamide-substituted 1,3,5-triphenyl pyrazoline compounds as a promising tyrosinase inhibitor agent. These compounds prepared by multicomponent reaction of corresponding aldehyde, ketone, and hydrazine using seal-vessel reactor. Pyrazolines compound were tested for their tyrosinase inhibitor activity through in vitro assay. The test result found that compound 4c, 4d, and 4e possessed better tyrosinase inhibitory activity compared to the reference drug, kojic acid. Compound 4c exhibited the strongest tyrosinase inhibitory effect with an IC50 value of 30.14 µM. The results suggested that hydroxyl and methoxy substituent at para position are preferable. Furthermore, molecular docking studies result match the pattern of in vitro assay where the compound will provide a stronger binding interaction and lower binding free energies.
Isolation and Molecular Identification of Amylolytic Bacteria from Oryctes rhinoceros L. Larvae Decomposing Empty Palm Oil Fruit Bunches
Authors: Uto Sahriani , Arfah Rugaiyah , Dali Seniwati , Baharuddin Maswati
Published: August 2023

Oryctes rhinoceros L. is an organism that helps the decomposition of oil palm empty fruit bunches (OPEFB). In the larvae's intestines, there are symbiotic bacteria that are used in the process of food degradation in the digestive system, one of which is amylolytic bacteria. This study aims to isolate and molecular identify amylolytic bacteria that produce amylase enzymes from horn beetle larvae. The techniques are used to screen and isolate bacteria from horn beetle larvae. Bacterial identification was accomplished by microscopically identifying amylase-producing bacterial isolates, performing biochemical tests on selected bacterial isolates, quantifying amylase enzyme activity, and molecularly identifying 16S rRNA. The results of screening and bacterial isolation obtained five isolates. The largest amylolytic bacterial colony index value was obtained in the EA3 isolate, which was 1.370 mm. Bacterial isolates with the highest activity were found in isolates coded EA1 and EA2, namely 0.049 U/mL and 0.0479 U/mL. According to the findings of 16S rRNA molecular identification, isolates EA1 and EA2 had similarities with the bacteria Ochrobactrum sp. and Pseudomonas mendocina.
Antibacterial Peptide from Chymotrypsin Hydrolysate of Jatropha Seeds with RP-HPLC Fractionation
Authors: Andriana Zehan , Wahyuningsih Tutik Dwi , Raharjo Tri Joko
Published: August 2023

Antimicrobial peptide (AMP), as a new antibiotic agent, has promising prospects in overcoming the problem of resistance. AMP production can be carried out by proteolytic enzymes. Protein from castor bean (R. communis) is toxic, so it can potentially be a source of AMP. This study aims to obtain protein hydrolysate from the castor bean (R. communis) using several extraction methods, including SDS dialysis, SDS-gel filtration, trifluoroacetic acid (TFA), and acetone precipitation. Chymotrypsin enzyme was used to hydrolyze the protein, and the peptide hydrolysate was fractionated using RP-HPLC. The peptide fraction was tested for its antibacterial activity by agar diffusion and microdilution methods, and the most active fraction was identified for its amino acid sequence by LC-HRMS. The results showed that the acetone precipitation extraction method was the best method, with a degree of hydrolysis of 83.9%. The active fractions 6 and 10 of RP-HPLC had IC50 values of 14.1 and 14.5 µg/mL for E. coli and 13.3 and 14.4 µg/mL for S. aureus, respectively. NVLRGKGMASL peptides were found in fraction 10, and GIILLSSK, NMIAKR, and LLDILTKK peptides were found in fraction 6 with an alpha helix secondary structure that can cause membrane damage. The peptides NVLRGKGMASL, GIILLSSK, NMIAKR, and LLDILTKK are thought to have potential as antibacterial compounds.
Phytochemical Constituent and Cytotoxic Activity of Eusideroxylon zwageri Teijsm & Binn Extract and Sub-Fraction on Human Breast Cancer Cell Line T47D
Authors: Kurniawan Yayan Rabbani , Santoni Adlis , Suryati Suryati
Published: August 2023

Eusideroxylon zwageri Teijsm. & Binn T has been reported to have bioactivity, one of which is the potential as an excellent cytotoxic agent using the BSLT method.  So far, stage tests using cancer cells and analysis of the content of secondary metabolites have not been reported, Therefore, it is essential to analyze the content of secondary metabolites using Liquid Chromatography – Tandem Mass Spectrometry (LC-MS/MS) and determination of cytotoxic activity using Brine Shrimp Lethality Test (BLST) mwthod with various solvent polarities. Then separation by bioassay-guided isolation using column chromatography of the active extract assay BSLT. Furthermore, the cytotoxic activity of the active extract and the active isolate was tested against T47D cancer cells with Microculture Tetrazolium (MTT) method. The results of the analysis of the content of secondary metabolites using LC-MS/MS identified senbusine B and 6,7-Dehydroartemisinic acid on hexane extract, while the ethyl acetate extract produced 4 compounds, and 15 compounds were identified in the methanol extract. The results of the cytotoxic activity test using the BSLT method on each extract showed that the hexane extract was the most active, with an LC50 of 17.56 mg/L. Furthermore, the cytotoxic activity of hexane extract against T47D cells showed weak activity with an IC50 value of 237.5 mg/L, while the AB1 sub-fraction showed moderate activity with an IC50 value of 138.4 mg/L. The results of the analysis using HPLC indicated that the active isolate in the form of sub-fraction AB1 contained the compound senbusine B.
Cytotoxic Potential of Essential Oil Isolated from Clibadium Surinamese L Leaves Against T47D Breast and HeLa Cervical Cancer Cells
Authors: Ulia Rahmi Vika , Suryati Suryati , Santoni Adlis
Published: August 2023

Semambu (C. surinamense L) plant is a shrub plant that is easy to find. Several terpenoid compounds have been isolated from this plant, previous studies have shown cytotoxic activity of terpenoid class compounds. Terpenoid compounds in a plant are mostly found in essential oils (monoterpenes and sesquiterpenes). So far, there has been no report on the cytotoxic potential of essential oils from the leaves of this plant. It is necessary to isolate the essential oils from C. surinamense L leaves and test their cytotoxic potential. Isolation of essential oil of C. surinamense L leaves was carried out by hydrodistillation method, the oil was obtained in the form of a light yellow liquid with a specific gravity of 0.968 g/mL. Analysis of chemical components with Gas Chromatography-Mass-Spectrometry (GC-MS) through comparison of data from the National Institute of Standards and Technologies (NIST) found that there were 55 compounds (monoterpene and sesquiterpene groups) with six main compounds, namely β-caryophyllene (30.4%), β-sesquiphellandrene (8.46%), 3 carene (8.16%), α-bisabolene (4.05%), α-humulene (4.0%), and epi- bicyclosesquiphellandrene (4.0%). The potential cytotoxic test of essential oil from isolation showed highly cytotoxic activity with the Brine Shrimp Lethality Test (BSLT) method against Artemia salina L shrimp larvae with LC50 value of 0.9261 μg/mL and Microculture tetrazolium test (MTT) method against T47D breast cancer cells and HeLa cervix with IC50 values of 12.72 μg/mL and 30.14 μg/mL.
Biochemical Characterization of Ketapang Lipase: Its Preference to Short-Chain Fatty Acids despite the Long-Chain Fatty Acids Dominant Content
Authors: Faradis Taritsu Hazal , Pomeistia Meilynda , Basri Nurul Hasan , 'Ardhuha Jannatin , Gunawan Erin Ryantin , Savalas Lalu Rudyat Telly
Published: August 2023

Lipases are versatile enzymes with high specificity toward lipid substrate. They have many industrial applications, such as in food, pharmacy, and green fuel. So far, most explored lipases are from microbial and animal sources, whereas those from plants are less studied. The present study aims to characterize ketapang (Terminalia catappa Linn) lipase. The lipase was isolated from germinating ketapang seeds. The activity was determined by hydrolysis of virgin coconut oil (VCO). Biochemical characterization of ketapang lipase includes the optimum temperature, pH, kinetics, metal ions addition, and analysis of substrate specificity. It was shown that ketapang lipase has an optimum temperature of 45 oC, pH 7.5. Ca2+ increases the lipase activity, whereas Na+, K+, Mg2+, Zn2+, Fe2+, and Cu2+ inhibit ketapang lipase to various extents. A comparison of SDS-PAGE and native-PAGE analysis showed that ketapang lipase consists of several protein subunits. A further test by in-gel assay revealed that the 54 kDa, 35 kDa, two bands at ~16 kDa, and 12 kDa proteins showed lipolytic activity against a-naphthyl palmitate substrate. When tested on various chromogenic fatty acid substrates, ketapang lipase showed the highest specificity against short-chain fatty acids (C4 and C8), despite the fact that ketapang oil seed composes mainly of long fatty acid (C18). Since lipases that have high lipolytic activity toward short fatty acids are considered esterases, the esterase activity of ketapang lipase is yet to be determined.
PENGARUH MODEL PEMBELAJARAN PROBLEM BASED LEARNING (PBL) DAN PROJECT BASED LEARNING (PjBL) TERHADAP KEMAMPUAN KOMUNIKASI MATEMATIS SISWA
Authors: Zakiyah Luluk , Supandi Supandi , Dwijayanti Ida
Published: August 2023

This study aims to determine the effect of Problem Based Learning (PBL) and Project Based Learning (PjBL) Learning Models on Students' Mathematical Communication Ability. The population in this study were all 8th grade students of SMP Negeri 1 Sukorejo in the 2021/2022 academic year. Samples were taken randomly as many as 3 classes, namely class A, B and D as the control class and the experimental class with simple random sampling technique. The research design used is Posttest-Only Control Design. The data collection used in this research is by observing, documenting, and giving written tests. The results of this study state that (1) There are differences in students' mathematical communication skills in learning mathematics using Problem Based Learning (PBL), Project Based Learning (PjBL) and conventional models. (2) Students' mathematical communication skills in Problem Based Learning (PBL) learning models are better than conventional learning models. (3) Students' mathematical communication skills in the Project Based Learning (PjBL) learning model are better than the conventional learning model. (4) There is no difference in students' mathematical communication skills in the Project Based Learning (PjBL) and Problem Based Learning (PBL) models.
STUDI KEMAMPUAN LITERASI NUMERASI PADA MOTIVASI BELAJAR SISWA DENGAN MODEL REALISTIC MATHEMATICS EDUCATION BERBANTUAN E-MODUL
Authors: Sari Vini Kartika , Pratikno Budi , Wahyuningrum Endang
Published: August 2023

The research discussed the numeracy literacy skills in terms of student learning motivation in realistic mathematics education (RME) based on e-modules. The methodology is a qualitative and quantitative research. The sample is drawn in simple random sampling, four randomly selected classes from the six existing classes. The questionnaires are focused on question of the numeracy literacy ability tests and student learning motivation. To test the effectiveness of RME learning, we  approaches using regression analysis, t tests of the independent mean on pair and one sample data. The results showed that student learning motivation has an influence on numeracy literacy ability, there is a significant difference between the numeracy literacy ability of students who are taught using RME learning methods and classical lecture learning methods, where students who are taught with RME have better numeracy literacy skills, namely completing the minimum completion criteria (KKM) compared to students who are taught using the classical lecture learning method. Student learning motivation in the realistic mathematics education learning approach has a more effective influence than student learning motivation in classical lecture learning
KONSTRUKSI LAMPU GANTUNG MENGGUNAKAN TABUNG, BOLA, TORUS, KERUCUT DENGAN KONSEP DEFORMASI, TRANSFORMASI DAN KURVA BEZIER
Authors: Juniar Vina Alpiani , Juliyanto Bagus , Ubaidillah Firdaus
Published: August 2023

The purpose of this study is to obtain a hanging light design from a combination of several components using Maple. The results show that the construction procedure for the hanging light components can be carried out in three stages. First, determining some data for cylinder, sphere, torus, cone, and Bezier curve. Second, dividing the hanging light into several components, then creating a procedure for constructing each component using the concepts of deformation, transformation, and Bezier curve. Third, combining all components on the modeling axis and compiling a program using a computer.
Kawasan Wisata Bahari di Pesisir Pantai Utara dan Selatan Provinsi Jawa Tengah: Sebuah Ulasan (Marine tourism areas on the north and south coasts of Central Java: a Review)
Authors: Widianto Luthfi , Larassari Permata
Published: August 2023

Indonesia merupakan sektor pariwisata oleh karenanya pemerintah Indonesia terus  menekankan pariwisata sebagai andalan sumber pendapatan nasional. Jawa Tengah sebagai provinsi dengan karakteristik garis pantai  yang  berbeda yaitu pantai utara dan pantai selatan,  memiliki  rencana pengembangan pariwisata dalam pemanfaatan pesisirnya. Potensi objek dan daya tarik di Provinsi Jawa Tengah sangat beragam dan tersebar di kabupaten/kota di Jawa Tengah. Wisata rekreasi berbasis alam menjadi daya tarik utama, terutama wisata Bahari pantai dan laut. Hal ini bertujuan untuk mengetahui pengelolaan Kawasan Wisata Bahari di pesisir provinsi Jawa Tengah. Analisis pengelolaan Kawasan wisata Bahari memiliki beberapa parameter yaitu aksesibilitas, sarana dan prasarana, dan keamanan. Pengelolaan  wisata  bahari  di  Provinsi Jawa Tengah menunjukkan tingkat keberlanjutan yang berbeda pada karakteristik pantai utara dan pantai selatan Provinsi Jawa Tengah. Berdasarkan hasil pengelolaan tingkat kunjungan di pantai Utara tertinggi yaitu kabupaten Rembang. Sedangkan tingkat kunjungan terendah yaitu Kabupaten Pati pada tahun 2020 dan untuk tahun 2021 kunjungan terendah yaitu Kabupaten Demak. Tinggi rendahnya kunjungan wisatawan di tiap Kawasan Wisata Bahari dipengaruhi oleh faktor aksesibilitas, sarana dan prasarana serta keamanan.Berdasarkan hasil tingkat kunjungan di perairan selatan jawa kunjungan tertinggi yaitu kabupaten Purworejo tahun 2020, sedangkan tahun 2021 yaitu kabupaten Cilacap. Untuk  tikgkat pengunujung terendah yaitu kabupaten Wonogiri. Strategi   pengelolaan yang  dapat diberikan yaitu  ketersediaan  peraturan  daerah mengenai    wisata    bahari,    sarana    listrik, teknik pengolahan  limbah,  penyerapan  tenaga  kerja,  dan daya  dukung  wisata  untuk  kategori  wisata  pantai.
Pengaruh Lama Waktu Kontak terhadap Kinerja Trickling Filter Dalam Menurunkan Kandungan Bahan Organik Limbah Cair Industri Roti
Authors: Nadya Nadya , Sani Nur Millah , Puspitasari Arum
Published: August 2023

Limbah cair industri roti mengandung bahan organik yang tinggi, ditunjukkan dengan tingginya nilai Biochemical Oxygen Demand (BOD), Chemical Oxygen Demand (COD) dan kekeruhan memiliki nilai yang melebihi standar baku mutu berdasarkan Perda Jawa Tengah No 5 Tahun 2012 dan Peraturan Menteri Kesehatan R.I. No : 416/MENKES/PER/IX/1990. Tujuan penelitian ini untuk mengetahui waktu kontak paling baik dan hasil pengolah sistem Trickling filter (TF) dalam menurunkan kandungan BOD, COD dan kekeruhan yang sesuai dengan standar baku mutu limbah cair yang sudah ditetapkan. Teknologi yang digunakan dalam penelitian ini adalah sistem TF. Metode yang digunakan adalah metode eksperimental menggunakan Rancangan Acak Lengkap (RAL) dengan menggunakan perbedaan waktu kontak (24, 48, 72, 96 dan 120). Hasil pengamatan awal limbah sebelum diolah adalah pada kadar BOD sebesar 2634,1 mg/L, kadar COD sebesar 4191,3 mg/L dan kekeruhan sebesar 1277 NTU. Dari hasil pengamatan diperoleh waktu kontak paling baik efektivitas penurunan kandungan BOD, COD dan kekeruhan berturut-turut sebesar 96,8%, 92,7% dan 96,9% pada waktu 120 jam. Waktu kontak efektif yang sesuai dengan standar baku mutu air limbah yaitu BOD 120 jam, COD 120 jam dan kekeruhan yang mendekati yaitu 120 jam.
A Wireless Animal Robot Stimulation System Based on Neuronal Electrical Signal Characteristics
Authors: Yan Rui , Qiu Zengrui , Chen Zhaozhen , Yang Junqing , Wang Hui , Cui Tianle , Huai Ruituo
Published: August 2023

As a stimulus signal, coded electrical signals can control the motion behavior of animals, which has been widely used in the field of animal robots. In current research, most of the stimulus signals used by researchers are traditional waveform, such as square waves. To enrich the stimulus waveform, a wireless animal robot stimulation system based on neuronal electrical signal characteristics is presented in this paper. The stimulator uses the CC1101 wireless module to control animal behavior through brain stimulation. The LabVIEW-based graphical user interface(GUI) can manipulate brain stimulation remotely while the stimulator powered by battery. Additionally, the spikes of animals have been simulated by this system through Direct Digital Synthesizer(DDS) algorithm. The GUI enable users to customize the combination of these analog spike signals. The recombined signals are sent to the stimulator through CC1101 as stimulus signals. In vivo experiments conducted on five pigeons verified the efficacy of the stimulation mechanism. The analog spike signal with an amplitude of 3-5V successfully caused the pigeon’s turning behavior. The feasibility of the analog spike signals as stimulus signals was successfully verified. Increased the diversity of stimulus waveform in the field of animal robots.
An Evaluation of the Resilience of Humanitarian Supply Chains in the Event of Flash Flooding
Authors: Xu Wenping , Li Wenzhuo , Procerbs David , Chen Wenbo
Published: August 2023

The sudden onset of natural hazards such as a flash flood events can be devastating to a country or region, often affecting numerous communities, and humanitarian supply chains play a major role in enabling the timely recovery of economic and social activities. However, the uncertainty and suddenness of natural disasters such as flash floods, as well as the potential for aftershocks, require humanitarian supply chains to be resilient during the relief process. This research first adopts the Delphi and literature review methods to identify the key indicators in the humanitarian supply chain to form an evaluation index system. Then, taking the 2021 mega-flash flood event which affected four cities in the mountainous region under the jurisdiction of the Zhengzhou municipal government, the ANP method is used to calculate the weights of each indicator and combines the weighted results with the Multi-styling and stereotyping method under Pythagorean fuzzy (PFs-VIKOR) to make an evaluation of the resilience of the humanitarian supply chain in the relief process. The findings suggest that policy makers and decision makers should pay close attention to the coordination of the parties involved in the humanitarian supply chain and improve the level of resilience of the entire supply chain by enhancing the resource scheduling capacity and responsiveness. At the same time, the VIKOR evaluation of the 4 cities highlighted the humanitarian supply chain in Dengfeng City to be most effective in this event due to the close cooperation and positive response of all parties involved in the humanitarian supply chain. The findings of this research provide some useful suggestions and guidance to the various practitioners involved in the humanitarian supply chain. Furthermore, the evaluation of the performance of the four cities in this mega-flash flood event provides some helpful indication of the importance of the various emergency measures which can help to inform policy recommendations for the Zhengzhou municipal government.
The Role of Neurophysiology in Managing Patients with Chiari Malformations
Authors: Moncho Dulce , Poca Maria A. , Rahnama Kimia , Sánchez-Roldán M.A. , Santa-Cruz Daniela , Sahuquillo Juan
Published: August 2023

Chiari malformation type 1 (CM1) includes a series of congenital anomalies that share ectopia of the cerebellar tonsils below the foramen magnum, in some cases associated with syringomyelia or hydrocephalus. CM1 can cause dysfunction of the brainstem, spinal cord, and cranial nerves. This functional alteration of the nervous system can be detected by various modalities of neurophysiological tests, such as brainstem auditory evoked potentials, somatosensory evoked potentials, motor evoked potentials, electromyography and nerve conduction studies of the cranial nerves and spinal roots, as well as brainstem reflexes. The main goal of this study is to review the findings of multimodal neurophysiological examinations in published studies of patients with CM1 and their indication in the diagnosis, treatment, and follow-up of these patients, as well as their utility in intraoperative monitoring.