bluuebunny/crossref_metadata_2025 huggingface dataset:
Overall 5-year survival rate and disease-free survival after segmentectomy versus lobectomy in patients with non-small cell lung cancer
Authors: Kadeetham Khunthorn , Ngodngamthaweesuk Montien , Kantathut Narongrit , Samankatiwat Piya , Cherntanomwong Piya , Leelayana Parinya , Khajarern Siam
Published: December 2022

Objectives:

Anatomical lobectomy has always been the standard operative treatment of early-stage non-small cell lung cancer. However, there have been emerging evidences suggesting that a subanatomical resection, such as segmentectomy, may yield the same treatment results, even in patients with higher-stage non-small cell lung cancer. This study aimed to compare overall 5-year survival rate and disease-free survival between lobectomy and segmentectomy in patients with non-small cell lung cancer.

Methods:

The retrospective study included 380 patients who underwent surgery for non-small cell lung cancer at Ramathibodi Hospital between 1st January 2016 and 31st December 2020. Of 380 patients, 307 patients underwent lobectomy, while the other 73 patients underwent segmentectomy. Operative, admission, and follow-up data were collected from electronic medical records. Missing data were collected by telephone calls to patients or their relatives in deceased cases. Overall and disease-free survival were analyzed.

Results:

Median overall 5-year survival time after lobectomy and segmentectomy seemed to be different but not statistically significant (18.5 months versus 5.8 months, p = 0.127). Median disease-free survival time after lobectomy and segmentectomy was also similar (8.6 months versus 4.5 months, p = 0.511). Two deaths occurred during perioperative period, one from lobectomy group due to acute massive pulmonary embolism (0.3%) and the other from segmentectomy group due to acute exacerbation of chronic obstructive pulmonary disease with respiratory failure (1.4%).

Conclusion:

Lobectomy and segmentectomy result in similar overall 5-year survival rate and disease-free survival between these two comparison groups. Therefore, segmentectomy may be a potential alternative for operative treatment of non-small cell lung cancer. However, a larger and randomized-controlled trial may be needed to further validate these results.
Immediate postpartum intrauterine contraceptive device utilization and associated factors among women who gave birth in public health facilities of Adama town, Ethiopia
Authors: Aemro Etenat , Abdo Meyrema , Deksisa Alem , Alemayehu Afework , Mulatu Teshale , Ahmed Hassen Tahir , Molla Alemayehu , Teji Roba Kedir
Published: December 2022

Objectives:

The evidence on utilization of immediate postpartum intrauterine contraceptive devices (IPPIUCDs) and its associated factors are limited in Ethiopia. Hence, this study intended to assess IPPIUCD utilization and related factors among women who gave birth in Adama town public hospitals, Ethiopia.

Method:

A facility-based cross-sectional study was done among 493 postpartum mothers at selected government health facilities in Adama town from January 20 to February 20, 2021. All women who gave birth in selected government health facilities and within 48 h of postpartum were included in the study. Data were collected using an interviewer-administered questionnaire. Logistic regression models were used to identify the factors associated with IPPIUCD utilization. Adjusted odds ratios (AORs) with 95% confidence interval (CI) were calculated to measure the strength of association and statistical significance was declared at p < 0.05.

Result:

In this study, 22.1% (95% CI: 17.3–25.2) of the mothers used IPPIUCDs within 48 h of giving birth. Having three or more children (AOR = 4.18, 95% CI: 1.79–9.79), having no desire to have another child (AOR = 3.9, 95% CI: 1.86–8.17), counseling after delivery (AOR = 3.1 95% CI: 1.52–6.34), and having good knowledge about PPIUCD (AOR = 3.82, 95% CI: 1.94–7.49) were significantly associated with IPPIUCD utilization.

Conclusion:

The utilization of IPPIUCD in this study was low. Strategies to raise pregnant mothers’ awareness of IPPIUCD through mass media, and integrating standard counseling on immediate postpartum intrauterine device (IPPIUD) during antenatal care, and the immediate postpartum period are required to improve IPPIUD utilization.
“This Isn’t Your Data, Friend”: Black Twitter as a Case Study on Research Ethics for Public Data
Authors: Klassen Shamika , Fiesler Casey
Published: December 2022

While research has been conducted with and in marginalized or vulnerable groups, explicit guidelines and best practices centering on specific communities are nascent. An excellent case study to engage within this aspect of research is Black Twitter. This research project considers the history of research with Black communities, combined with empirical work that explores how people who engage with Black Twitter think about research and researchers in order to suggest potential good practices and what researchers should know when studying Black Twitter or other digital traces from marginalized or vulnerable online communities. From our interviews, we gleaned that Black Twitter users feel differently about their content contributing to a research study depending on, for example, the type of content and the positionality of the researcher. Much of the advice participants shared for researchers involved an encouragement to cultivate cultural competency, get to know the community before researching it, and conduct research transparently. Aiming to improve the experience of research for both Black Twitter and researchers, this project is a stepping stone toward future work that further establishes and expands user perceptions of research ethics for online communities composed of vulnerable populations.
Tribal Perspectives on Hypertension: Results From the Center for Native American Health Native-CHART Needs Assessment
Authors: Parker Tassy , Kelley Allyson , Cooeyate Norman , Tsosie Nathania
Published: December 2022

Purpose and Objectives:

American Indian/Alaska Native (AI/AN) hypertension contributes to cardiovascular disease, the leading cause of premature death in this population. The purpose of this article is to document strategies, concerns, and barriers related to hypertension and cardiovascular disease from Native-Controlling Hypertension and Risks through Technology (Native-CHART) symposiums facilitated by the Center for Native American Health (CNAH). The objectives of this evaluation were to combine Health Needs Assessment (HNA) data and explore barriers and strategies related to hypertension while assessing changes in participants’ perspectives over time (2017-2021).

Approach:

CNAH followed an iterative process each year for planning the HNA, facilitating the HNA, and refining and reflecting on HNA findings over time. This involved 3 interconnected steps: (1) developing a shared understanding for the HNA, “Why are we here?,” (2) facilitating the HNA during annual symposiums “What do we do?,” and (3) reflecting on “What did we learn?”.

Evaluation Methods:

Data were collected using a culturally centered HNA co-created by the CNAH team and tribal partners. Qualitative data analysis utilized a culturally centered thematic approach and NVivo software version 12.0. Quantitative data analysis included summarizing frequency counts and descriptive statistics using Microsoft Excel.

Results:

Over the 5-year period, 212 Native-CHART symposium participants completed HNAs. Data collected from HNAs show persistent barriers and concerns and illuminate potential strategies to address AI/AN hypertension. Future efforts must explore effective strategies that build on community strengths, culture and traditions, and existing resources. This is the path forward.

Implications for Public Health:

CNAH’s culturally centered and unique HNA approach helped assess participant perspectives over time. CNAH facilitated symposiums over multiple years, even amid a global pandemic. This demonstrates resilience and continuity of community outreach when it is needed the most. Other universities and tribal partners could benefit from this iterative approach as they work to design HNAs with tribal populations.
Smell and Taste in Children with Covid-19
Authors: Meliante Piero Giuseppe , Marcellino Alessia , Altomari Roger , Testa Alessia , Gallo Andrea , Ralli Massimo , de Vincentiis Marco , Lubrano Riccardo
Published: December 2022

Objectives

To assess the frequency of loss of smell and taste in children during Covid-19 infection and their prevalence along with other symptoms, as well as the recovery of chemosensory function once healed.

Methods

To evaluate symptoms during infection, we adapted the Scandinavian adaptation of the Multi-Clinic Smell and Taste Questionnaire and the modified Monel-Jefferson questionnaire. For smell analysis we used Odor Identification (OI) and two variants of the Odor Discrimination (OD) test, and we compared the results with those of a control group.

Results

We enrolled nine patients in our experimental group and nine in our control group. Fever was the most frequent symptom (55% of cases), followed by anosmia and ageusia (44% of cases), muscle pain and asthenia (22% of cases) and diarrhea, abdominal pain, cough, and headache (11% of cases). In 11% of cases, olfactory symptoms were the only manifestation of the disease. There was no statistically significant difference in OI test and OD tests between the two groups (Children healed from Covid-19 and Control Group).

Conclusion

Loss of smell and taste are the second most common symptoms of pediatric Covid-19, and they should always be tested because they can be the only manifestations of infection. Olfactory function in Covid-19 children decreases with increasing age and improves with the passage of time after illness.
Developing an Extended Theory of UTAUT 2 Model to Explore Factors Influencing Taiwanese Consumer Adoption of Intelligent Elevators
Authors: Chu Tzu-Hsin , Chao Cheng-Min , Liu Hsieh-Hsi , Chen Der-Fa
Published: December 2022

Followed by the fast developments in Internet of Things (IoT), artificial intelligence (AI) technologies, utilizing cloud architecture and big data, have been integrated into various aspects of our daily lives. Such an integration results in high levels of convenience, comfort, and energy savings. Deploying such new technologies benefits the elevator industry as well. Intelligent elevator with energy control schemes can improve the energy efficiency of eco-friendly applications. The purpose of this study is to examine the factors that affect the intelligent elevator adoption of Taiwanese people using the extended unified theory of acceptance and use of technology (UTAUT 2) model, which considers factors such as environmental consciousness, artificial intelligence optimism, attitude, and perceived quality. A well-structured face-to-face survey is performed to obtain data from the consumers living in central Taiwan. The data acquired are analyzed via structural equation modeling using the partial least squares method. The analysis results indicate that attitude, habit, performance expectancy, effort expectancy, and perceived quality are important factors that affect the behavioral intentions of consumers when adopting new technologies, such as an intelligent elevator. However, facilitating conditions adversely affect the behavioral intentions. In addition, environmental consciousness and artificial intelligence optimism are crucial factors that affect performance and effort expectancies, whereas the latter two contribute positively to attitude. The findings of this study supplement existing literature regarding the behavioral intentions for adopting intelligent elevators. Finally, the theoretical and practical significances of this research study are discussed.
Does the Chronic Stress of Everyday Discrimination or Race Itself Better Predict AD Onset Risk?
Authors: Gary Katharine M. , Hoque Masudul , Yashkin Arseniy P. , Yashin Anatoliy I. , Akushevich Igor
Published: December 2022

Using evidence from the Health and Retirement Study, we explore racial disparities in Alzheimer’s Disease (AD) onset risk. From a stress process perspective, there is substantial evidence in the literature that everyday discrimination is a chronic strain for Black individuals that acts as a social determinant of illness. However, few studies have examined specific relationships between this social stressor, race, and AD onset risk. Using Cox Proportional Hazard Models, we examined racial differences in exposure and vulnerability to everyday discrimination. Findings suggest that everyday discrimination predicts AD onset risk, and Black individuals experience more frequent exposure to everyday discrimination as a chronic strain. However, contrary to the stress process model, Black respondents were not more vulnerable to the effect of everyday discrimination on AD onset risk. Racial bias from medical professionals during the diagnostic process and mortality selection bias may explain this effect. Overall, the results of this study provide further evidence that discrimination is a key factor in predicting AD while also considering that many racial minorities with high rates of this type of social stress may not receive an unbiased diagnosis and/or survive to late life to develop AD.
Social Justice and Public Health: A Framework for Curriculum Reform
Authors: Munala Leso , Allen Elizabeth M. , Beall Oona M. , Phi Kieu My
Published: December 2022

As public health aims to improve health outcomes of all populations, social justice is argued to be the philosophy on which public health is based. A social justice approach to population health requires the field to recognize that the inequities in society have negative consequences for health. The purpose of this work is to develop a framework for integrating social justice into a public health curriculum. We used an interprofessional approach to create a definition of social justice, and develop a training program and evaluation plan. The program weaves social justice throughout the core MPH curriculum. The social justice definition is the foundation of the curriculum which is composed of eight key social justice domains with competencies directly aligned with each of the domains. Our evaluation plan focuses on students’ change in understanding of social justice in public health. The overarching goal is to explicitly integrate social justice into an MPH curriculum such that students can work toward health equity and justice in their careers.
RCT versus real-world cohorts: Differences in patient characteristics drive associations with outcome after EVT
Authors: Quandt Fanny , Meißner Nina , Wölfer Teresa A , Flottmann Fabian , Deb-Chatterji Milani , Kellert Lars , Fiehler Jens , Goyal Mayank , Saver Jeffrey L , Gerloff Christian , Thomalla Götz , Tiedt Steffen
Published: December 2022

Background:

The selection of patients with large-vessel occlusion (LVO) stroke for endovascular treatment (EVT) depends on patient characteristics and procedural metrics. The relation of these variables to functional outcome after EVT has been assessed in numerous datasets from both randomized controlled trials (RCT) and real-world registries, but whether differences in their case mix modulate outcome prediction is unknown.

Methods:

We leveraged data from individual patients with anterior LVO stroke treated with EVT from completed RCTs from the Virtual International Stroke Trials Archive ( N = 479) and from the German Stroke Registry ( N = 4079). Cohorts were compared regarding (i) patient characteristics and procedural pre-EVT metrics, (ii) these variables’ relation to functional outcome, and (iii) the performance of derived outcome prediction models. Relation to outcome (functional dependence defined by a modified Rankin Scale score of 3–6 at 90 days) was analyzed by logistic regression models and a machine learning algorithm.

Results:

Ten out of 11 analyzed baseline variables differed between the RCT and real-world cohort: RCT patients were younger, had higher admission NIHSS scores, and received thrombolysis more often (all p < 0.0001). Largest differences at the level of individual outcome predictors were observed for age (RCT: adjusted odds ratio (aOR), 1.29 (95% CI, 1.10–1.53) vs real-world aOR, 1.65 (95% CI, 1.54–1.78) per 10-year increments, p < 0.001). Treatment with intravenous thrombolysis was not significantly associated with functional outcome in the RCT cohort (aOR, 1.64 (95 % CI, 0.91–3.00)), but in the real-world cohort (aOR, 0.81 (95% CI, 0.69–0.96); p for cohort heterogeneity = 0.056). Outcome prediction was more accurate when constructing and testing the model using real-world data compared to construction with RCT data and testing on real-world data (area under the curve, 0.82 (95% CI, 0.79–0.85) vs 0.79 (95% CI, 0.77–0.80), p = 0.004).

Conclusions:

RCT and real-world cohorts considerably differ in patient characteristics, individual outcome predictor strength, and overall outcome prediction model performance.
The EffecTs of Amlodipine and other Blood PREssure Lowering Agents on Microvascular FuncTion in Small Vessel Diseases (TREAT-SVDs) trial: Study protocol for a randomised crossover trial
Authors: Kopczak Anna , Stringer Michael S , van den Brink Hilde , Kerkhofs Danielle , Blair Gordon W , van Dinther Maud , Onkenhout Laurien , Wartolowska Karolina A , Thrippleton Michael J , Duering Marco , Staals Julie , Middeke Martin , André Elisabeth , Norrving Bo , Bousser Marie-Germaine , Mansmann Ulrich , Rothwell Peter M , Doubal Fergus N , van Oostenbrugge Robert , Biessels Geert Jan , Webb Alastair JS , Wardlaw Joanna M , Dichgans Martin
Published: December 2022

Background:

Hypertension is the leading modifiable risk factor for cerebral small vessel diseases (SVDs). Yet, it is unknown whether antihypertensive drug classes differentially affect microvascular function in SVDs.

Aims:

To test whether amlodipine has a beneficial effect on microvascular function when compared to either losartan or atenolol, and whether losartan has a beneficial effect when compared to atenolol in patients with symptomatic SVDs.

Design:

TREAT-SVDs is an investigator-led, prospective, open-label, randomised crossover trial with blinded endpoint assessment (PROBE design) conducted at five study sites across Europe. Patients aged 18 years or older with symptomatic SVD who have an indication for antihypertensive treatment and are suffering from either sporadic SVD and a history of lacunar stroke or vascular cognitive impairment (group A) or CADASIL (group B) are randomly allocated 1:1:1 to one of three sequences of antihypertensive treatment. Patients stop their regular antihypertensive medication for a 2-week run-in period followed by 4-week periods of monotherapy with amlodipine, losartan and atenolol in random order as open-label medication in standard dose.

Outcomes:

The primary outcome measure is cerebrovascular reactivity (CVR) as determined by blood oxygen level dependent brain MRI signal response to hypercapnic challenge with change in CVR in normal appearing white matter as primary endpoint. Secondary outcome measures are mean systolic blood pressure (BP) and BP variability (BPv).

Discussion:

TREAT-SVDs will provide insights into the effects of different antihypertensive drugs on CVR, BP, and BPv in patients with symptomatic sporadic and hereditary SVDs.

Funding:

European Union’s Horizon 2020 programme.

Trial registration:

NCT03082014.
Hearing Loss Screening, Diagnosis, and Treatment for Refugees and Asylees in an Urban Clinic, 2014‐2017
Authors: Jaradeh Katrin , Liao Elizabeth , Dieterich Cristy , Truong Sammi , Anand Payal , Chan Dylan K. , Raphael Eva
Published: December 2022

Objectives

First, to determine whether using a single‐question subjective hearing screen vs gold standard audiometric evaluation is effective for hearing loss screening in refugees and asylees. Second, to understand the clinical pathways for hearing loss diagnosis and treatment.

Study Design

This is a case series with chart review from January 2014 to December 2017.

Setting

A large urban safety net primary care clinic in San Francisco, California.

Methods

Patients were included who had a medical record and completed single‐question subjective hearing screening and audiometric evaluation during refugee health examinations. An overall 349 patients met all inclusion criteria.

Results

Out of 349 patients, 48% were male; the median age was 29.3 years (SD, 15.1). The majority came from Central or South America (n = 148, 42%) and China (n = 79, 23%). Among all patients, 10 (3%) failed the subjective hearing screen, and 18 (5%) failed audiometric evaluation. Of those who failed the subjective hearing screen, 4 (40%) passed audiometric evaluation. Of those who failed the audiometric evaluation, 12 (66%) passed subjective screening, and only 5 (28%) received a diagnostic audiogram, with 4 diagnosed with hearing loss and 1 receiving hearing aids. The sensitivity of the subjective screening question was 33% and the specificity 99% as compared with audiometric evaluation.

Conclusion

Audiometric evaluation is relatively inexpensive and easily administered, while a single subjective question is a poor screening tool. Hearing loss is undertreated in this population. Ensuring appropriate hearing loss screening, diagnosis, and treatment in this population is paramount to improving quality of life.
The eternal return: Imagining security futures at the Doomsday Vault
Authors: Harrington Cameron
Published: December 2022

This article examines how imaginaries of security in the Anthropocene function at the Svalbard Global Seed Vault (SGSV), otherwise known as the ‘Doomsday Vault’. Recent explorations by scholars of security have suggested that different ways of seeing, understanding, acting in, and imagining the world are necessary to adequately respond to complex crises in the Anthropocene. The dissolution of the nature/culture divide and the existential risk from planetary threats are said to require new and creative formations of security. Buried in the Norwegian high Arctic, the heavily fortified SGSV was built in 2008 as a planetary-scale, ‘deep-time organisation’ that would forever secure a wide variety of plant seeds and their genetic makeup against regional or global upheavals. The article argues that his seed ‘ark’ materialises three Anthropocene security imaginaries: apocalypse, hope and escape. The prevalence and use of these imaginaries reveal the stability of long-held security logics and challenge the widely-held belief in the innately transformative properties of the Anthropocene concept for security. Instead, the SGSV demonstrates the difficulty in overcoming a collective mindfulness that fixes security to eternal forms even in the midst of unprecedented threats, interventions and technology.
Get Closer to the World of Contact Sites: A Beginner's Guide to Proximity-Driven Fluorescent Probes
Authors: Poggio Elena , Brini Marisa , Calì Tito
Published: December 2022

To maintain cellular homeostasis and to coordinate the proper response to a specific stimulus, information must be integrated throughout the cell in a well-organized network, in which organelles are the crucial nodes and membrane contact sites are the main edges. Membrane contact sites are the cellular subdomains where two or more organelles come into close apposition and interact with each other. Even though many inter-organelle contacts have been identified, most of them are still not fully characterized, therefore their study is an appealing and expanding field of research. Thanks to significant technological progress, many tools are now available or are in rapid development, making it difficult to choose which one is the most suitable for answering a specific biological question. Here we distinguish two different experimental approaches for studying inter-organelle contact sites. The first one aims to morphologically characterize the sites of membrane contact and to identify the molecular players involved, relying mainly on the application of biochemical and electron microscopy (EM)-related methods. The second approach aims to understand the functional importance of a specific contact, focusing on spatio-temporal details. For this purpose, proximity-driven fluorescent probes are the experimental tools of choice, since they allow the monitoring and quantification of membrane contact sites and their dynamics in living cells under different cellular conditions or upon different stimuli. In this review, we focus on these tools with the purpose of highlighting their great versatility and how they can be applied in the study of membrane contacts. We will extensively describe all the different types of proximity-driven fluorescent tools, discussing their benefits and drawbacks, ultimately providing some suggestions to choose and apply the appropriate methods on a case-to-case basis and to obtain the best experimental outcomes.
Influence of rheologically weak layers on fault architecture: insights from analogue models in the context of the Northern Alpine Foreland Basin
Authors: Zwaan Frank , Schreurs Guido , Madritsch Herfried , Herwegh Marco
Published: December 2022

We present a series of analogue models inspired by the geology of the Zürcher Weinland region in the Northern Alpine Foreland Basin of Switzerland to explore the influence of rheological weak, i.e. (partially) ductile layers on the 3D evolution of tectonic deformation. Our model series test the impact of varying weak layer thickness and rheology, as well as different kinematics of an underlying “basal fault”. Model analysis focuses on deformation in the weak layer overburden and, uniquely, within the weak layer itself. We find that for low to moderate basal fault displacements, the above-mentioned parameters strongly influence the degree of coupling between the basal fault and the weak layer overburden. Coupling between the basal fault and overburden decreases by reducing the strength of the weak layer, or by increasing the weak layer’s thickness. As a result, basal fault displacement is less readily transferred through the weak layer, leading to a different structural style in the overburden. By contrast, increasing the amount, or rate, of basal fault slip enhances coupling and leads to a more similar structural style between basal fault and overburden. Moreover, dip-slip displacement on the basal fault is more readily transferred to the overburden than strike-slip displacement of the same magnitude. Our model results compare fairly well to natural examples in the Northern Alpine Foreland Basin, explaining various structural features. These comparisons suggest that rheological weak layers such as the Jurassic Opalinus Clay have exerted a stronger control on fault zone architecture than is commonly inferred, potentially resulting in vertical fault segmentation and variations in structural style. Furthermore, the novel addition of internal marker intervals to the weak layer in our models reveals how complex viscous flow within these layers can accommodate basal fault slip. Our model results demonstrate the complex links between fault kinematics, mechanics and 3D geometries, and can be used for interpreting structures in the Alpine Foreland, as well as in other settings with similar weak layers and basal faults driving deformation in the system.
Metabolic disorders sensitise endometrial carcinoma through endoplasmic reticulum stress
Authors: Zhou Jingyi , Lin Yanying , Yang Xiao , Shen Boqiang , Hao Juan , Wang Jiaqi , Wang Jianliu
Published: December 2022

Background

Metabolic disorder is considered a well-established risk factor for endometrial carcinoma (EC). However, the mechanism remains unclear. Insulin resistance and excessive flux of free fatty acids serve as fundamental pathogenic factors in metabolic disorders, including obesity and type 2 diabetes. The aim of this study was to test the correlation between insulin resistance and dyslipidaemia in EC and to determine the effect of insulin and saturated fatty acids on EC cells.

Methods

A retrospective study on the medical records of patients with EC and RNA-seq from the TCGA database analysed with edgR and Gene Ontology (GO) were used to assess the correlation of dyslipidaemia and diabetes as well as obesity. Crystal violet assays and CCK-8 assays were used to detect the proliferation of EC cells, and Annexin V-PI was used to examine apoptosis. Transient changes in mitochondrial Ca2+ and reactive oxygen species (ROS) were monitored via confocal microscopy. DNA damage was assessed by comet assays. Changes in signalling pathways were detected via phospho-kinase array. western blotting was used to assess the molecular changes in endoplasmic reticulum (ER) stress and DNA damage.

Results

We found that glucose metabolism disorders accompanied dyslipidaemia in patients with EC. As a key regulator of glucose metabolism disorders, insulin promoted DNA damage, ROS and Ca2+ homoeostasis imbalance in a panel of established EC cell lines. Interestingly, excessive insulin boosted saturated fatty acid-induced pro-apoptotic effects in EC cells. Furthermore, our data showed that insulin synergised with saturated fatty acids to activate the mechanistic target of rapamycin kinase/70 kDa ribosomal protein S6 kinase (mTOR/p70S6K) pathway and ER stress, resulting in Ca2+ release from ER and unfolded protein response (UPR) activation, which contributed to combined insulin and saturated fatty acid treatment-induced apoptosis and tumour progression.

Conclusions

Our data are the first to illustrate that impaired glucose metabolism accelerates dyslipidaemia-promoted EC progression, which is attributed to hyperinsulinaemia and saturated fatty acid-induced Ca2+ dyshomoeostasis and UPR activation in EC cells via ER stress.
Unparalleled details of soft tissues in a Cretaceous ant
Authors: Zhuang Yuhui , Xu Wenjing , Zhang Guojie , Mai Huijuan , Li Xiaoqin , He Hong , Ran Hao , Liu Yu
Published: December 2022

For social insects such as ants, the internal organs are likely important in understanding their eusocial behavior and evolution. Such organs, however, are rarely preserved on fossils. In each of the few cases reporting exceptionally fossilized soft tissues in arthropods, the nervous, muscular and cardiovascular systems have been described individually, but never in combination. Here, we report a female specimen (gyne) of the extinct ant group—†Zigrasimecia—included in a Cretaceous amber piece from Kachin, Myanmar, with an almost complete system formed by various internal organs. These include the brain, the main exocrine system, part of the digestive tract, and several muscle clusters. This research expands our knowledge of internal anatomy in stem group ants. As the gyne bears a morphologically unique labrum, our specimen’s internal and external features support the notion that the early ant may have special ecological habits during the Cretaceous period.
Genomic diversity and comprehensive taxonomical classification of 61 Bacillus subtilis group member infecting bacteriophages, and the identification of ortholog taxonomic signature genes
Authors: Abraha Haftom Baraki , Lee Jae-Won , Kim Gayeong , Ferdiansyah Mokhammad Khoiron , Ramesha Rathnayaka Mudiyanselage , Kim Kwang-Pyo
Published: December 2022

Background

Despite the applications of Bacillus subtilis group species in various sectors, limited information is available regarding their phages. Here, 61 B. subtilis group species-infecting phages (BSPs) were studied for their taxonomic classification considering the genome-size, genomic diversity, and the host, followed by the identification of orthologs taxonomic signature genes.

Results

BSPs have widely ranging genome sizes that can be bunched into groups to demonstrate correlations to family and subfamily classifications. Comparative analysis re-confirmed the existing, BSPs-containing 14 genera and 21 species and displayed inter-genera similarities within existing subfamilies. Importantly, it also revealed the need for the creation of new taxonomic classifications, including 28 species, nine genera, and two subfamilies (New subfamily1 and New subfamily2) to accommodate inter-genera relatedness. Following pangenome analysis, no ortholog shared by all BSPs was identified, while orthologs, namely, the tail fibers/spike proteins and poly-gamma-glutamate hydrolase, that are shared by more than two-thirds of the BSPs were identified. More importantly, major capsid protein (MCP) type I, MCP type II, MCP type III and peptidoglycan binding proteins that are distinctive orthologs for Herelleviridae, Salasmaviridae, New subfamily1, and New subfamily2, respectively, were identified and analyzed which could serve as signatures to distinguish BSP members of the respective taxon.

Conclusions

In this study, we show the genomic diversity and propose a comprehensive classification of 61 BSPs, including the proposition for the creation of two new subfamilies, followed by the identification of orthologs taxonomic signature genes, potentially contributing to phage taxonomy.
Root system architecture of historical spring wheat cultivars is associated with alleles and transcripts of major functional genes
Authors: Maqbool Saman , Ahmad Suhaib , Kainat Zarnishal , Khan Muhammad Ibrar , Maqbool Ammarah , Hassan Muhammad Adeel , Rasheed Awais , He Zhonghu
Published: December 2022

We evaluated root system architecture (RSA) of a set of 58 historical spring wheat cultivars from Pakistan representing 105 years of selection breeding. The evaluations were carried out under control and water-limited conditions using a high-throughput phenotyping system coupled with RhizoVision Explorer software. The cultivars were classified into three groups based on release year as cultivars released pre-1965, released between 1965 and 2000, and cultivars released post-2000. Under water-limited conditions a decline in 20 out of 25 RSA component traits was observed in pre-1965 cultivars group. Whereas cultivars released after the 1965, so-called green revolution period, showed a decline in 17 traits with significant increments in root length, depth, and steep angle frequency which are important root traits for resource-uptake under water-limited conditions. Similarly, cultivars released after 2000 indicated an increase in the number of roots, depth, diameter, surface area, and steep angle frequency. The coefficient of correlation analysis showed a positive correlation between root depth and yield-related traits under water-limited conditions. We also investigated the effects of green-revolution genes (Rht1) and some phenology-related genes such as DRO1, TaMOR, TaLTPs, TaSus-2B on RSA and identified significant associations of these genes with important root traits. There was strong selection pressure on DRO1 gene in cultivated wheat indicating the allele fixed in modern wheat cultivars is different from landraces. The expression of DRO1, and TaMOR were retrieved from an RNAseq experiment, and results were validated using qRT-PCR. The highest expression of DRO1 and TaMOR was found in Chakwal-50, a rainfed cultivar released in 2008, and MaxiPak-65 released in 1965. We conclude that there is a positive historic change in RSA after 1965 that might be attributed to genetic factors associated with favored RSA traits. Furthermore, we suggest root depth and steep angle as promising traits to withstand water-limited environments and may have implications in selection for breeding.
Transcriptome profiling provides insights into leaf color changes in two Acer palmatum genotypes
Authors: Zhu Lu , Wen Jing , Ma Qiuyue , Yan Kunyuan , Du Yiming , Chen Zhu , Lu Xiaoyu , Ren Jie , Wang Yuelan , Li Shushun , Li Qianzhong
Published: December 2022

Background

Ornamental trees with seasonally-dependent leaf color, such as Acer palmatum, have gained worldwide popularity. Leaf color is a main determinant of the ornamental and economic value of A. palmatum. However, the molecular mechanisms responsible for leaf color changes remain unclear.

Results

We chose A. palmatum cultivars with yellow (‘Jinling Huangfeng’) and red (‘Jinling Danfeng’) leaves as the ideal material for studying the complex metabolic networks responsible for variations in leaf coloration. The 24 libraries obtained from four different time points in the growth of ‘Jinling Huangfeng’ and ‘Jinling Danfeng’ was subjected to Illumina high-throughput sequencing. We observed that the difference in cyanidin and delphinidin content is the primary reason behind the varying coloration of the leaves. Transcriptomic analyses revealed 225,684 unigenes, and the Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of differentially expressed genes (DEGs) confirmed that they were involved in ‘anthocyanin biosynthesis.’ Eighteen structural genes involved in anthocyanin biosynthesis were thought to be related to anthocyanin accumulation, whereas 46 MYBs, 33 basic helix-loop-helixs (bHLHs), and 29 WD40s were presumed to be involved in regulating anthocyanin biosynthesis. Based on weighted gene co-expression network analysis (WGCNA), three candidate genes (ApRHOMBOID, ApMAPK, and ApUNE10) were screened in the significant association module with a correlation coefficient (r2) of 0.86.

Conclusion

In this study, the leaf color changes of two A. palmatum genotypes were analyzed. These findings provide novel insights into variations in leaf coloration and suggest pathways for targeted genetic improvements in A. palmatum.
The Registrar Clinical Encounters in Training (ReCEnT) cohort study: updated protocol
Authors: Davey Andrew , Tapley Amanda , van Driel Mieke , Holliday Elizabeth , Fielding Alison , Ball Jean , Mulquiney Katie , Fisher Katie , Spike Neil , Clarke Lisa , Moad Dominica , Ralston Anna , Patsan Irena , Mundy Benjamin , Turner Alexandria , Tait Jordan , Tuccitto Lucrezia , Roberts Sarah , Magin Parker
Published: December 2022

Background

During vocational general practice training, the content of each trainee’s (in Australia, registrars’) in-consultation clinical experience is expected to entail a breadth of conditions that exemplify general practice, enabling registrars to gain competency in managing common clinical conditions and common clinical scenarios. Prior to the Registrar Clinical Encounters in Training (ReCEnT) project there was little research into the content of registrars’ consultations despite its importance to quality of training. ReCEnT aims to document the consultation-based clinical and educational experiences of individual Australian registrars.

Methods

ReCEnT is an inception cohort study. It is comprised of closely interrelated research and educational components. Registrars are recruited by participating general practice regional training organisations. They provide demographic information about themselves, their skills, and their previous training. In each of three 6-month long general practice training terms they provide data about the practice where they work and collect data from 60 consecutive patient encounters using an online portal. Analysis of data uses standard techniques including linear and logistic regression modelling. The ReCEnT project has approval from the University of Newcastle Human Research Ethics Committee, Reference H-2009–0323.

Discussion

Strengths of the study are the granular detail of clinical practice relating to patient demographics, presenting problems/diagnoses, medication decisions, investigations requested, referrals made, procedures undertaken, follow-up arranged, learning goals generated, and in-consultation help sought; the linking of the above variables to the presenting problems/diagnoses to which they pertain; and a very high response rate. The study is limited by not having information regarding severity of illness, medical history of the patient, full medication regimens, or patient compliance to clinical decisions made at the consultation. Data is analysed using standard techniques to answer research questions that can be categorised as: mapping analyses of clinical exposure; exploratory analyses of associations of clinical exposure; mapping and exploratory analyses of educational actions; mapping and exploratory analyses of other outcomes; longitudinal ‘within-registrar’ analyses; longitudinal ‘within-program’ analyses; testing efficacy of educational interventions; and analyses of ReCEnT data together with data from other sources. The study enables identification of training needs and translation of subsequent evidence-based educational innovations into specialist training of general practitioners.
Serum soluble T cell immunoglobulin mucin domain-3 as an early predictive marker for severity of acute pancreatitis; a retrospective analysis
Authors: Wang Fushuang , Zhu Minghui , Meng Yao , Lin Min
Published: December 2022

Background

Early prediction of severe acute pancreatitis (SAP) plays an important role in timely treatment decisions. Soluble T cell immunoglobulin and mucin domain-3 (sTIM-3) has been applied as a potential biomarker for the prediction of many diseases, while its predictive ability for AP severity remains largely unexplored. In this study, we aimed to identify whether serum sTIM-3 could be used as an indicator of AP severity in the early stage of the disease.

Methods

A retrospective study was conducted. The enrolled AP patients should meet the 2012 Atlanta guideline and have an onset to admission ≤ 48 h.

Results

A total of 94 AP patients were enrolled in the current analysis, including 42 (45%), 35 (37%), and 17 (18%) patients were diagnosed as mild AP (MAP), moderately SAP (MSAP), and SAP, respectively. SAP patients had significantly higher the white blood cells (WBCs) count, red blood cells (RBCs) count, C-reactive protein (CRP) level, direct bilirubin level, creatinine and procalcitonin levels compared with MAP and MSAP patients. Among SAP and MSAP patients, significantly higher APACHE II, BISAP, and MCTSI scores were observed compared with MAP patients, and there was significant difference in APACHE II and BISAP scores between SAP and MSAP patients. Stepwise multivariate linear regression analysis showed that the concentrations of serum sTIM-3, as well as the BISAP and MCTSI scores, were significantly associated with the severity of AP. The areas under the ROC curve were 0.914 (95% CI, 0.865-0.963), 0.855 (95%CI, 0.742-0.968) 0.853 (95%CI, 0.768-0.938), and 0.746 (95%CI, 0.633-0.860) for BISAP score, APACHE II score, sTIM-3 level, and MCTSI score, respectively.

Conclusions

Serum sTIM-3 might be ultimately incorporated into a predictive system for assessing the severity of AP.
Application of a WeChat-based mini-app as a patient reminder in Helicobacter pylori eradication: a prospective multi-center randomized controlled study
Authors: Sun Kefang , Chen Yishu , Wang Zhenzhen , Liu Yi , Pan Yue , Mao Xinli , Xu Lei , Jin Chaohui , Chen Ming , Yu Chaohui , Li Lan
Published: December 2022

Background

To improve the eradication rate of H. pylori, researchers have investigated the role of WeChat-based mini-app as an electronic reminding system in H. pylori treatment.

Methods

Subjects from three medical centers were divided into two groups. Patients in the daily mini-app-based notification system group received daily notifications via the WeChat mini-app. Patients in the control group received one-time verbal education on the first clinical visit. Both groups received a 14-day quadruple therapy to eradicate H. pylori infection. Eradication rate, compliance, adverse events and satisfaction were evaluated.

Results

Both intention-to-treat (ITT) and per-protocol (PP) analyses were conducted. The eradication rate in the daily mini-app-based notification system group was slightly higher compared with the control group (ITT analysis: 76.70% vs. 70.73%, p = 0.312; PP analysis: 85.87% vs. 82.86%, p = 0.562). The compliance was significantly higher in the daily mini-app-based notification system group (ITT analysis: 85.52% vs. 70.48%, p = 0.028; PP analysis: 92.39% vs. 81.90%, p = 0.030). The adverse event rates were similar between the two groups (PP analysis: 36.96% vs. 40.95%, p = 0.566). No significant difference in eradication rate was seen in each subgroup analysis by age, place of residence, grade of education, or endoscopic findings.

Conclusion

The study showed that daily mini-app-based notification improved patient compliance but not H. pylori eradication rate.

Trial registration The research was registered in the Chinese Clinical Trial Registry (ChiCTR2000031011, 21/03/2020).
Clinical characteristics and risk factors for a prolonged length of stay of patients with asymptomatic and mild COVID-19 during the wave of Omicron from Shanghai, China
Authors: Hu Chen , Liu Yu-Kai , Sun Qi-Di , Du Zheng , Fang Yu-Qiang , Guo Fei , Wang Yu-Bo , He Yong , Cen Yuan , Zeng Fan
Published: December 2022

Background

This study aims to investigate the clinical characteristics and the length of hospital stay (LOS), as well as risk factors for prolonged LOS in a cohort of asymptomatic and mild COVID-19 patients infected with the Omicron variant.

Methods

A total of 1166 COVID-19 patients discharged from the inpatient ward of the largest makeshift hospital (May 8–10, 2022) in Shanghai, China, were included. The demographics, medical history, and the lowest and admission cycle threshold (Ct) values of the RT-PCR tests for SARS-CoV-2 genes of the open reading frame 1ab (Ct-ORF) and the nucleocapsid protein (Ct-N) during hospitalization were recorded. Patients with LOS > 7 days, or LOS ≤ 7 days were included in the Prolonged group or the Control group, separately. The clinical characteristics and LOS of the participants in the two groups were described and compared. Multivariate Logistic and linear regression analyses were applied to explore the risk factors for prolonged LOS. The diagnostic efficacy of the lowest and admission Ct values for the Prolonged group was tested via the receiver operating characteristic (ROC) curve analysis.

Results

The median LOS was 6 days in the total study population. The age was older (45.52 ± 14.78 vs. 42.54 ± 15.30, P = 0.001), while both the lowest and admission Ct-ORF (27.68 ± 3.88 vs. 37.00 ± 4.62, P < 0.001; 30.48 ± 5.03 vs. 37.79 ± 3.81, P < 0.001) and Ct-N (25.79 ± 3.60 vs. 36.06 ± 5.39, P < 0.001; 28.71 ± 4.95 vs. 36.95 ± 4.59, P < 0.001) values were significantly lower in the Prolonged group. There were more mild cases in the Prolonged group (23.8% vs. 11.5%, P < 0.001). The symptom spectrum differed between the two groups. In multivariate analyses, age, disease category, and the lowest Ct-N values were shown to be associated with prolonged LOS. Besides, both the lowest and admission Ct-ORF (AUC = 0.911 and 0.873) and Ct-N (AUC = 0.912 and 0.874) showed robust diagnostic efficacy for prolonged LOS.

Conclusions

Our study firstly reports the clinical characteristics and risk factors for prolonged LOS during the wave of the Omicron epidemic in Shanghai, China. These findings provide evidence for the early identification of asymptomatic and mild COVID-19 patients at a high risk of prolonged hospitalization who may require early intervention, and long-term monitoring and management.
Tender cervical lymphadenitis as a herald of multi-system inflammatory syndrome in COVID-19 infection of children and adolescents: a report of two cases
Authors: Wanninayake Leonard , de Abrew Gayathree , Logeshwaran Dinusha , Weerasinghe Chathurika , Gowinna Piyumi , Mettananda Sachith , Premaratna Ranjan
Published: December 2022

Background

Post-COVID-19 multisystem inflammatory syndrome (MIS) has been increasingly recognized but fever with isolated tender cervical lymphadenitis as the initial presentation has been rarely reported. We present 2 female patients one a child and the other an adolescent.

Case presentation

Case 1 was a 13-year-old girl who presented with tender cervical lymphadenopathy and fever 3-weeks post-COVID-19, and then developed features of MIS 5 days later. Case 2, also female, was 18 years old. She had no history of COVID-19 infection or immunization but had a serologic diagnosis of COVID-19. She similarly presented with fever and tender cervical lymphadenopathy, and then progressed rapidly to develop features of MIS. Both patients responded well to treatment with immunosuppressants and intravenous immunoglobulin.

Conclusion

Tender cervical lymphadenopathy could be the herald of multi-system inflammatory syndrome following COVID-19 infection among children and adolescents, which the clinicians must have a good suspicion about.