bluuebunny/crossref_metadata_2025 huggingface dataset:
Response of Acanthamoeba castellanii mitochondria to oxidative stress.
Authors: Trocha Lidia K , Stobienia Olgierd
Published: November 2019

The purpose of this study was to examine the effects of oxidative stress caused by hydroperoxide (H(2)O(2)) in the presence of iron ions (Fe(2+)) on mitochondria of the amoeba Acanthamoeba castellanii. We used isolated mitochondria of A. castellanii and exposed them to four levels of H(2)O(2) concentration: 0.5, 5, 15, and 25 mM. We measured basic energetics of mitochondria: oxygen consumption in phosphorylation state (state 3) and resting state (state 4), respiratory coefficient rates (RC), ADP/O ratios, membrane potential (DeltaPsi(m)), ability to accumulate Ca(2+) , and cytochrome c release. Our results show that the increasing concentrations of H(2)O(2) stimulates respiration in states 3 and 4. The highest concentration of H(2)O(2) caused a 3-fold increase in respiration in state 3 compared to the control. Respiratory coefficients and ADP/O ratios decreased with increasing stress conditions. Membrane potential significantly collapsed with increasing hydroperoxide concentration. The ability to accumulate Ca(2+) also decreased with the increasing stress treatment. The lowest stress treatment (0.5 mM H(2)O(2)) significantly decreased oxygen consumption in state 3 and 4, RC, and membrane potential. The ADP/O ratio decreased significantly under 5 mM H(2)O(2) treatment, while Ca(2+) accumulation rate decreased significantly at 15 mM H(2)O(2). We also observed cytochrome c release under increasing stress conditions. However, this release was not linear. These results indicate that as low as 0.5 mM H(2)O(2) with Fe(2+) damage the basic energetics of mitochondria of the unicellular eukaryotic organism Acanthamoeba castellanii.
Metabolic transformations of antitumor imidazoacridinone, C-1311, with microsomal fractions of rat and human liver.
Authors: Wiśniewska Anita , Chrapkowska Agnieszka , Kot-Wasik Agata , Konopa Jerzy , Mazerska Zofia
Published: November 2019

The imidazoacridinone derivative C-1311 is an antitumor agent in Phase II clinical trials. The molecular mechanism of enzymatic oxidation of this compound in a peroxidase model system was reported earlier. The present studies were performed to elucidate the role of rat and human liver enzymes in metabolic transformations of this drug. C-1311 was incubated with different fractions of liver cells and the reaction mixtures were analyzed by RP-HPLC. We showed that the drug was more sensitive to metabolism with microsomes than with cytosol or S9 fraction of rat liver cells. Incubation of C-1311 with microsomes revealed the presence of four metabolites. Their structures were identified as dealkylation product, M0, as well as a dimer-like molecule, M1. Furthermore, we speculate that the hydroxyl group was most likely substituted in metabolite M3. It is of note that a higher rate of transformation was observed for rat than for human microsomes. However, the differences in metabolite amounts were specific for each metabolite. The reactivity of C-1311 with rat microsomes overexpressing P450 isoenzymes, of CYP3A and CYP4A families was higher than that with CYP1A and CYP2B. Moreover, the M1 metabolite was selectively formed with CYP3A, whereas M3 with CYP4A. In conclusion, this study revealed that C-1311 varied in susceptibility to metabolic transformation in rat and human cells and showed selectivity in the metabolism with P450 isoenzymes. The obtained results could be useful for preparing the schedule of individual directed therapy with C-1311 in future patients.
In vitro plant tissue cultures accumulate polyisoprenoid alcohols.
Authors: Skorupinska-Tudek Karolina , Pytelewska Anna , Zelman-Femiak Monika , Mikoszewski Jakub , Olszowska Olga , Gajdzis-Kuls Dorota , Urbanska Natalia , Syklowska-Baranek Katarzyna , Hertel Józefina , Chojnacki Tadeusz , Swiezewska Ewa
Published: November 2019

In vitro cultivated plant cells and tissues were found to synthesize polyisoprenoids. Taxus baccata suspension cell cultures accumulated polyisoprenoids of the same pattern as the parental tissue; methyl jasmonate or chitosan treatment almost doubled their content. All the root cultures studied accumulated dolichols as predominant polyisoprenoids. Roots of Ocimum sanctum grown in vitro accumulated approx. 2.5-fold higher amount of dolichols than the roots of soil-grown plants. Dolichols dominated over polyprenols in all Triticum sp. tissues studied.
Synthesis and anti-HIV properties of novel 6-phenylselenenyl-5-propyluracils.
Authors: Miazga Agnieszka , Felczak Krzysztof , Siwecka Maria A , Lipniacki Andrzej , Piasek Andrzej , Kulikowski Tadeusz
Published: November 2019

Novel 6-phenylselenenyl-5-propyluracils were synthesized from 5-propyluracil with the use of regioselective synthesis to give 1-[(2-hydroxyethoxy)-methyl]-6-phenylselenenyl-5-propyluracil (6), 1-ethoxymethyl-6-phenylselenenyl-5-propyluracil (9) and 1-benzyloxymethyl-6-phenylselenenyl-5-propyluracil (10). Interaction of these compounds with recombinant HIV-1 reverse transcriptase (RT) was evaluated using a non-isotopic colorimetric method. Compounds 9 and 10 exerted potent HIV RT inhibition (IC(50) 0.06 and 0.05 microM respectively) while compound 6 showed moderate inhibition (IC(50) = 3.5 microM). Potent anti-HIV-1 activity in MT-2 cells inoculated by a syncythia-inducing HIV-1 (cat #3 strain) laboratory isolate was exerted by compounds 9 and 10 (EC(50) 0.62 microM and 0.025 microM, respectively), while compound 6 showed only moderate activity (IC(50) = 4.1 microM). In addition, compound 10 showed very good in vitro therapeutic index (TI > 2046), indicating that it is a potential anti-HIV/AIDS drug.
The DnaK chaperones from the archaeon Methanosarcina mazei and the bacterium Escherichia coli have different substrate specificities.
Authors: Zmijewski Michal A , Skórko-Glonek Joanna , Tanfani Fabio , Banecki Bogdan , Kotlarz Agnieszka , Macario Alberto J L , Lipińska Barbara
Published: November 2019

Hsp70 (DnaK) is a highly conserved molecular chaperone present in bacteria, eukaryotes, and some archaea. In a previous work we demonstrated that DnaK from the archaeon Methanosarcina mazei (DnaK(Mm)) and the DnaK from the bacterium Escherichia coli (DnaK(Ec)) were functionally similar when assayed in vitro but DnaK(Mm) failed to substitute for DnaK(Ec) in vivo. Searching for the molecular basis of the observed DnaK species specificity we compared substrate binding by DnaK(Mm) and DnaK(Ec). DnaK(Mm) showed a lower affinity for the model peptide (a-CALLQSRLLS) compared to DnaK(Ec). Furthermore, it was unable to negatively regulate the E. coli sigma32 transcription factor level under heat shock conditions and poorly bound purified sigma32, which is a native substrate of DnaK(Ec). These observations taken together indicate differences in substrate specificity of archaeal and bacterial DnaKs. Structural modeling of DnaK(Mm) showed some structural differences in the substrate-binding domains of DnaK(Mm) and DnaK(Ec), which may be responsible, at least partially, for the differences in peptide binding. Size-exclusion chromatography and native gel electrophoresis revealed that DnaK(Mm) was found preferably in high molecular mass oligomeric forms, contrary to DnaK(Ec). Oligomers of DnaK(Mm) could be dissociated in the presence of ATP and a substrate (peptide) but not ADP, which may suggest that monomer is the active form of DnaK(Mm).
High-resolution structure of NodZ fucosyltransferase involved in the biosynthesis of the nodulation factor.
Authors: Brzezinski Krzysztof , Stepkowski Tomasz , Panjikar Santosh , Bujacz Grzegorz , Jaskolski Mariusz
Published: November 2019

The fucosyltransferase NodZ is involved in the biosynthesis of the nodulation factor in nitrogen-fixing symbiotic bacteria. It catalyzes alpha1,6 transfer of l-fucose from GDP-fucose to the reducing residue of the synthesized Nod oligosaccharide. We present the structure of the NodZ protein from Bradyrhizobium expressed in Escherichia coli and crystallized in the presence of phosphate ions in two crystal forms. The enzyme is arranged into two domains of nearly equal size. Although NodZ falls in one broad class (GT-B) with other two-domain glycosyltransferases, the topology of its domains deviates from the canonical Rossmann fold, with particularly high distortions in the N-terminal domain. Mutational data combined with structural and sequence alignments indicate residues of potential importance in GDP-fucose binding or in the catalytic mechanism. They are all clustered in three conserved sequence motifs located in the C-terminal domain.
Orientation and spectral properties of two stilbazolium merocyanine dyes in stretched and unstretched polyvinyl alcohol films.
Authors: Dudkowiak Alina , Frackowiak Danuta , Teślak Ewa , Gryczyński Zygmunt , Gryczyński Ignacy
Published: November 2019

Spectral properties (anisotropy coefficients calculated for absorption, emission and fluorescence decay time) of two stilbazolium merocyanine dyes have been determined to evaluate the applicability of these dyes as sensitizers in photodynamic therapy. The dyes were embedded in an anisotropic polymer matrix. Analysis of the emission decay components measured in polarized light provides information on the interactions of the dye molecules with the polymer matrix being a model of an anisotropic biological system. Different values of the emission anisotropies obtained from various polarized components of fluorescence decays have shown that the orientations of the dye molecules influence their interactions with the polymer. This means that differently oriented dye molecules located in biological systems should exhibit different interactions with membranes. The chain length and type of side groups attached as well as the salt form of the dye molecule were shown to influence the dye-polymer interactions and should be taken into account before the application of merocyanine dyes in medicine. These dyes seem to be promising optical sensors with spectral properties, including the calculated anisotropy coefficients, sensitive to the molecular environment, useful to study orientation and interaction with neighbouring molecules in biological membranes.
Role of N-acetyl-beta-d-hexosaminidase in cholesteatoma tissue.
Authors: Olszewska Ewa , Olszewski Slawomir , Borzym-Kluczyk Malgorzata , Zwierz Krzysztof
Published: November 2019

Cholesteatoma is a destructive disease characterized by the progressive expansion of keratinizing squamous epithelium in the middle ear and mastoid, and chronic inflammatory reaction of the subepithelial connective tissue. N-Acetyl-beta-d-hexosaminidase (HEX) catalyzes the release of terminal non-reducing N-acetyl-d-hexosamine residues acting on glucosides and galactosides in glycoproteins, G(M2)-gangliosides and glycosaminoglycans (GAGs). In this study the activities of HEX were measured in cholesteatoma tissue and in normal skin to demonstrate a possible role of HEX in bone resorption in the area adjacent to cholesteatoma. Cholesteatomas (n = 21) and normal adult retroauricular skin (controls, n = 21), were collected from patients during surgery due to chronic otitis media. In 20 of 21 specimens a significantly higher activity of HEX was observed in cholesteatoma tissue compared with that in normal skin. Mean release of HEX from the activated cells was 68.55 +/- 30.77 nkat/g wet tissue in cholesteatoma and 31.79 +/- 10.02 nkat/g wet tissue in skin specimens. It may explain the process of bone resorption in the area adjacent to cholesteatoma, i.e. ossicles or temporal bone. This study suggests that drugs inhibiting HEX activity, such as iminocyclitols, may be useful in cholesteatoma treatment.
Purification and characterization of GlcNAc-6-P 2-epimerase from Escherichia coli K92.
Authors: Ferrero Miguel Angel , Martínez-Blanco Honorina , Lopez-Velasco Federico Felino , Ezquerro-Sáenz Carlos , Navasa Nicolas , Lozano Sofia , Rodríguez-Aparicio Leandro B
Published: November 2019

N-Acetylmannosamine (ManNAc) is the first committed intermediate in sialic acid metabolism. Thus, the mechanisms that control intracellular ManNAc levels are important regulators of sialic acid production. In prokaryotic organisms, UDP-N-acetylglucosamine (GlcNAc) 2-epimerase and GlcNAc-6-P 2-epimerase are two enzymes capable of generating ManNAc from UDP-GlcNAc and GlcNAc-6-P, respectively. We have purified for the first time native GlcNAc-6-P 2-epimerase from bacterial source to apparent homogeneity (1 200 fold) using Butyl-agarose, DEAE-FPLC and Mannose-6-P-agarose chromatography. By SDS/PAGE the pure enzyme showed a molecular mass of 38.4 +/- 0.2 kDa. The maximum activity was achieved at pH 7.8 and 37 degrees C. Under these conditions, the K(m) calculated for GlcNAc-6-P was 1.5 mM. The 2-epimerase activity was activated by Na(+) and inhibited by mannose-6-P but not mannose-1-P. Genetic analysis revealed high homology with bacterial isomerases. GlcNAc-6-P 2-epimerase from E. coli K92 is a ManNAc-inducible protein and is detected from the early logarithmic phase of growth. Our results indicate that, unlike UDP-GlcNAc 2-epimerase, which promotes the biosynthesis of sialic acid, GlcNAc-6-P 2-epimerase plays a catabolic role. When E. coli grows using ManNAc as a carbon source, this enzyme converts the intracellular ManNAc-6-P generated into GlcNAc-6-P, diverting the metabolic flux of ManNAc to GlcNAc.
Menadione effect on l-cysteine desulfuration in U373 cells.
Authors: Wróbel Maria , Jurkowska Halina
Published: November 2019

The non-cytotoxic concentration (20 microM) of menadione (2-methyl-1,4-naphthoquinone), after 1 h of incubation, leads to loss of the activity of rhodanese by 33%, 3-mercaptopyruvate sulfurtransferase by 20%, as well as the level of sulfane sulfur by about 23% and glutathione by 12%, in the culture of U373 cells, in comparison with the control culture. Reactive oxygen species generated by menadione oxidize sulfhydryl groups in active centers of the investigated enzymes, inhibiting them and saving cysteine for glutathione synthesis. A decreased sulfane sulfur level can be correlated with an oxidative stress.
The Comparison of Hemodynamic Responses Following Laryngeal Mask Airway Insertion Versus Tracheal Intubation in Hypertensive Patients Scheduled for Elective Ophthalmic Surgery Under General Anesthesia
Authors: Sanatkar Mehdi , Goudarzi Mehrdad , Ebrahim Soltani Alireza
Published: November 2019

We compared hemodynamic responses following laryngeal mask airway insertion versus tracheal intubation in hypertensive patients who were scheduled for elective ophthalmic surgery under general anesthesia. We studied 48 controlled hypertensive patients that were randomly divided into two groups (n=24) for insertion of laryngeal mask airway (LMA) and endotracheal intubation (EI). The mean arterial blood pressure (MAP), heart rate, rate pressure product (RPP), and ST-segment changes were recorded preoperatively, immediately preintubation and 1, 3, and 5 minutes after LMA insertion or tracheal intubation in all patients and compared between two groups. There was a reduction in MAP after induction and immediately preintubation in all of patients of both groups (P<0.05). The MAP, heart rate and RPP increased immediately after both LMA insertion and tracheal intubation (P<0.05). The elevation of MAP and RPP were maintained for longer time in intubation group versus LMA group (P<0.05). There was no difference between the groups with respect to ST-segment variation. The incidence of airway injury was similar between two groups. The laryngeal mask airway insertion may be preferable to endotracheal intubation in hypertensive patients where attenuation of hemodynamic stress response is desired.
© 2019 Tehran University of Medical Sciences. All rights reserved.
Acta Med Iran 2019;57(5):289-294.
The Effect of Pre-Emptive Dose of Intravenous Ketamine on Oculocardiac Reflex in Pediatric Strabismus Surgery: A Randomized Clinical Trial
Authors: Goudarzi Mehrdad , Mehrpour Mohammad , Saleh Rajabi Mohammad , Sanatkar Mehdi , Espahbodi Ebrahim , Akbari Mohammad Reza
Published: November 2019

To evaluate the effect of pre-emptive dose of intravenous ketamine on Oculocardiac reflex (OCR) in pediatric strabismus surgery. 60 children with age between 2 to 8 years old candidate for strabismus surgery were randomly divided into two groups of 30 participants. After induction of anesthesia, in one group, pre-emptive dose of ketamine (0.15 mg/kg) was given intravenously (ketamine group), while the other group was given the same volume of normal saline (control group). During the surgery, OCR was recorded if the heart rate had decreased 20% or more from the baseline. In the recovery room, the incidence of nausea and vomiting, bronchospasm, laryngospasm, hallucination and recovery nurse satisfaction score were recorded. The incidence of OCR in total participants was 31 (51.7%). In control group 21 (70%) patients had OCR during surgery while this incidence was 10 (33.3%) in ketamine group which was significantly lower (P:0.004). the incidence of postoperative nausea and vomiting and the mean of recovery nurse satisfaction score in recovery room were not significantly different between two groups. The hallucination was not seen in any cases of two groups after surgery. The pre-emptive dose of ketamine (0.15 mg/kg) can reduce incidence of OCR in the pediatric strabismus surgery.
© 2019 Tehran University of Medical Sciences. All rights reserved.
Acta Med Iran 2019;57(5):295-298.
Lived Experiences of Undergraduate Medical Students About Hidden Curriculum: A Phenomenological Study
Authors: Bigdeli Shoaleh , Koohestani Hamid Reza , Soltani Arabshahi Seyed Kamran , Keshavarzi Mohammad Hasan
Published: November 2019

The hidden curriculum refers to student experiences that occur outside the formal structure of the curriculum, and especially the messages provided by the education system concerning values, perspectives, behaviors, and attitudes which has a strong influence on students' professional development. This study aimed at explaining the lived experiences of undergraduate medical students about the “hidden curriculum.” This was a qualitative phenomenological study. Participants were medical students of Iran University of Medical Sciences and they were chosen by purposive sampling method based on the inclusion criteria. Semi-structured interviews with open-ended questions were conducted with 10 students until data saturation, and 7-step Colaizzi's method was utilized for the purposes of analyzing the study findings. In the process of analyzing data from medical students' lived experiences about hidden curriculum, the following three themes were extracted: 1. Conflict in value beliefs (sub-themes: not giving priority to students education, applicability to the clinical practice of  basic sciences, attitude towards discipline, professional ethics, justice in evaluation”) 2. Modeling teacher’s communication skills patterns (sub-themes: “teacher-patient relationship,” “teacher -student relationship and professor-colleagues relationship) 3. Effective teaching (sub-themes: “clinical teaching” and “theoretical teaching”). Based on the results of the study, the hidden curriculum has both positive and negative impacts on various aspects of learning, especially the attitudinal domain. Consequently, paying specific attention to hidden curriculums is an absolute need.
© 2019 Tehran University of Medical Sciences. All rights reserved.
Acta Med Iran 2019;57(5):308-315.
Diphenhydramine Definitely Suppresses Fentanyl-Induced Cough During General Anesthesia Induction: A Double-Blind, Randomized, and Placebo-Controlled Study
Authors: Pourfakhr Pejman , Ziaei Seyed Hashem , Etezadi Farhad , Sharifinia Mohamadreza , Khajavi Mohammad Reza
Published: November 2019

Fentanyl-induced cough (FIC) is a known complication, and many studies have been conducted to prevent it. The aim of this study was to evaluate the effectiveness of Diphenhydramine as an antihistamine in suppressing of FIC during induction of anesthesia. In a prospective, double-blind, randomized controlled trial, a total of 100 patients, ASA Class I and II, scheduled for elective laparoscopy surgery were randomly assigned into two equally sized groups (n=50). Diphenhydramine diluted with distilled water as 10 mg/ml. Then, patients in Group D, received diphenhydramine 30 mg (3 ml) through peripheral IV line within 1 min and Group C received the same volume normal saline 0.9% as placebo. Two min later, fentanyl 2 µg/kg was administered through the peripheral IV line within 5 sec in all patients. The occurrence and intensity of cough within 2 min after the fentanyl injection were observed and recorded by a resident who was blinded to the study groups. The frequency of PONV, analgesic requirement in the recovery room and as a secondary outcome were recorded. The incidences of FIC were 47% in the control group, and there is no cough in the diphenhydramine group (P=0.02). The frequency of PONV was also reduced in diphenhydramine group (16% vs. 40%) and less number of patients in diphenhydramine group was needed to analgesia in the recovery room (60% vs. 82%). Our study determines that diphenhydramine (30 mg, IV) bolus injection 2 min before fentanyl injection can prevent FIC and PONV and also reduce analgesic requirement inthe recovery room.
© 2019 Tehran University of Medical Sciences. All rights reserved.
Acta Med Iran 2019;57(5):316-319.
Bupropion as a Treatment for Sexual Dysfunction Among Chronic Kidney Disease Patients
Authors: Ghoreishi Abolfazl , Dashtaki Lila , Hajisalimi Bahareh
Published: November 2019

Sexual dysfunction is a common complication among male patients with chronic kidney disease. Common disturbances include erectile dysfunction, decreased libido, and infertility. Sexual dysfunction is a multifactorial problem, and the treatment options are limited, it associated with lower quality of life scores in patients. Chronic kidney disease also has a critically impairing effect on the quality of life. To investigate the efficacy of bupropion on sexual dysfunction and quality of life in men with chronic kidney disease, a single-blind placebo-controlled trial was conducted. A total of 40 male patients with chronic kidney disease suffering from erectile dysfunction (Mean age 41/25±8/8) were randomly assigned to receive 10 weeks of treatment with either bupropion or placebo. Sexual function and quality of life were assessed by IIEF5 and WHOQOL-BREF questionnaires, respectively. Baseline demographic and clinical features were similar in both groups. The results showed a significant difference between the intervention and control groups in sexual function (P=0/005) and total quality of life (P=0/001); also the difference was significant in physical health (P=0/012), psychological health (P<0/001) and social relationship (P<0/001) domains. Our findings suggest that Bupropion is effective and safe for treating sexual dysfunction in men with chronic kidney disease and also could positively affect the quality of life among the patients.
© 2019 Tehran University of Medical Sciences. All rights reserved.
Acta Med Iran 2019;57(5):320-327.
Exactitud de la actividad de la telomerasa para el diagnóstico del virus del papiloma humano en mujeres con patología cervical en Armenia, Colombia, 2007
Authors: Ruiz-Hoyos Bayron Manuel , Loango-Chamorro Nelsy , Landázuri Patricia
Published: November 2019

Introducción: el cáncer de cérvix es un problema mundial de salud pública, con cerca de 500.000 casos diagnosticados anualmente, 68.000 de ellos en América Latina. En Colombia es la neoplasia maligna ginecológica más mortal y la segunda en incidencia después del cáncer de mama. La tasa de falsos negativos de la citología de cérvix es de 20-40%, lo que explica los casos presentes en la población oportunamente tamizada; la tipificación del virus del papiloma humano (VPH) y la actividad de la telomerasa son estudios promisorios en el diagnóstico temprano.Objetivo: determinar la exactitud de la actividad de telomerasa al compararla con la PCR para el VPH 16 o 18 en cérvix de mujeres con patología cervical.Materiales y métodos: estudio de exactitud diagnóstica ensamblado en un corte transversal, en 102 mujeres con sospecha de patología cervical que consultaron al Centro de Salud de primer nivel de atención de la Universidad del Quindío en 2007. Se evaluó la sensibilidad y especificidad de la telomerasa y la sensibilidad y especificidad de la PCR del VPH para lesión preneoplásica del cérvix.Resultados: la prevalencia de VPH 16 y 18 fue del 23,5 y 29% respectivamente. La sensibilidad de la telomerasa para el virus 16 fue del 93% con una especificidad del 39%. La sensibilidad de la telomerasa para el virus 18 fue del 96% con una especificidad del 37%.Conclusiones: la telomerasa surge como una prueba diagnóstica promisoria para la detección de los virus VPH 16 y 18.
Complicaciones en 748 histerectomías por laparoscopia utilizando un manipulador uterino con resaltador vaginal
Authors: Argüello-Argüello Ramiro
Published: November 2019

Introducción: la histerectomía laparoscópica (HL) requiere utilizar un dispositivo que permite una sección segura de la cúpula vaginal. El autor desarrolló un manipulador uterino con resaltador vaginal (M-R) para este paso. El objetivo de este trabajo es presentar las complicaciones asociadas a la utilización del M-R en diez años de uso.Materiales y métodos: se presenta una cohorte de mujeres sometidas a histerectomía laparoscópica en quienes se utilizó el manipulador uterino con resaltador vaginal (M-R) entre diciembre 7 de 1999 y junio 21 de 2011. El criterio de inclusión fue indicación de histerectomía por patología benigna. Se excluyeron aquellas con indicación de histerectomía vaginal, sospecha de adherencias intraabdominales o úteros excesivamente grandes. Se evaluó la edad, la paridad, el antecedente de cesáreas o laparotomías previas, el tiempo quirúrgico, el peso de la pieza quirúrgica, las complicaciones intra o posoperatorias y la evolución durante un seguimiento mínimo de 30 días posteriores a la cirugía.Resultados: en el periodo de observación se operaron 854 pacientes. En 106 pacientes el seguimiento posoperatorio fue menor a un mes. Los datos presentados corresponden a 748 pacientes. La edad promedio de las pacientes fue de 44 años (± 6,6). Las principales indicaciones de HL fueron miomatosis y adenomiosis uterina. Tiempo quirúrgico promedio: 90 min (± 17,7). Peso uterino promedio: 205 g (± 88,3). Se presentaron complicaciones en 41 pacientes (5,6%), de las cuales seis (0,8%) fueron complicaciones mayores: tres lesiones vesicales (0,4%), una lesión ureteral (0,13%), una paciente (0,13%) requirió laparoscopia operatoria por sangrado posquirúrgico, y una paciente (0,13%) laparotomía exploratoria por dolor abdominal. La tasa de conversión a laparotomía fue de 2%.Conclusión: con el uso del M-R se presentaron tasas de complicaciones similares a las reportadas por otros autores.
El control de la estabilidad en la función pública en Chile
Authors: Harris Moya Pedro
Published: November 2019

La estabilidad ha sido afirmada por largo tiempo como una garantía de la función pública en el derecho chileno. No obstante, la forma como se concreta el control de su cumplimiento ha variado sustancialmente en los últimos años. Este trabajo tiene por objeto examinar dicha variación respecto del personal que menor estabilidad ha tenido en este ámbito, como es el caso de los funcionarios transitorios y del personal a honorarios, a través de la aplicación del principio de confianza legítima por la jurisprudencia administrativa, y del denominado principio del contrato realidad por la jurisprudencia judicial.
Penerapan Metode Steepest Ascent Hill Climb pada Permainan Puzzle
Authors: Anam Hairul , Hanafi Feby Sabilhul , Adifia Ahmad Fauzal , Ababil Ahmad Firdaus , Bukhori Saiful
Published: November 2019

Puzzle is one example of the application of artificial intelligence, in the process of completion there are many search algorithms that can be applied. The 8 puzzle solution will be faster obtained if the array principle is used with a variation of the Steepest-Ascent Hill Climbing (Hill Climbing algorithm by choosing the sharpest / steepest slope) with the correct heuristic parameters and distance heuristics and combined with LogList as the storage state ever passed to overcome the problems in the hill climbing algorithm itself and avoid the looping state that has been passed. Steepest Ascent Hill Climbing is an algorithm method that is widely used for optimization problems. The application of the SAHC (Steepest Ascent Hill Climbing) Algorithm to the puzzle is needed so that the game is completed with optimal time.
Peningkatan Pengetahuan Gempa Dan Kemampuan Mitigasi Pada Siswa Disabilitas Kota Gorontalo Melalui Program Mie Titi
Authors: Duwingik Reskiyanto Fauzi , P. Kasim Della Nawarita , Ayu Dewi Ayu , Dano Mohamad Aristo , Manyoe Intan Noviantari
Published: November 2019

Kondisi Kota Gorontalo memiliki tingkat kerentanan yang tinggi terhadap bahaya gempa bumi, oleh karena itu pemahaman tentang gempa bumi perlu dilakukan sejak dini, terutama bagi anak-anak penyandang disabilitas. MIE TITI bertujuan untuk meningkatkan pengetahuan dan kemampuan mitigasi gempabumi bagi siswa disabilitas di SDLB Kota  Gorontalo, khususnya tunanetra, tunarungu, dan tunagrahita. Metode yang akan digunakan adalah metode fun learning dengan instrumen yang digunakan berupa MINING (Music Fun Learning), MICARD (Mitigation Flascard), MINAME (Mitigation of Nature Game) dan MIGAS (Mitigation Praise). Sampel sebanyak 34 orang penyandang disabilitas di SDLB Kota Gorontalo yang terdiri dari siswa tunanetra, tunarungu dan tunagrahita. Hasil yang diperoleh adalah terjadinya peningkatan pengetahuan gempa dan kemampuan mitigasi gempa pada siswa disabilitas dimana pada anak tunanetra sebesar 50% dan 35%, tunarungu sebesar 45% dan 55%, dan tunagrahita sebesar 3% dan 2%.
Kata Kunci: Pengetahuan, Mitigasi, Gempa, Fun Learning, Disabilitas, Gorontalo.
Penggunaan Sari Umbi Bawang Merah pada Pembibitan Pepaya
Authors: Ichsan Faryska Nur , Purnomo Djoko , Darsana Linajanti
Published: November 2019

People awareness toward nutritious food through the consumption of fruit is rising. Therefore, it needs the increasing of seed availability, either from the planting expansion or cultivation technology development. The productive life of papaya is around 3 years, so the rejuvenate of plant takes relatively quickly. However, the obstacle occurred comes from the adequate life of seed availability and the limitedness of homogeneous seed. Therefore, young seed forced to be used in the field will cause the growth of the plant not in unison. Moreover, it is also not profitable if it is considered from the cultivation of seedlings, since the price of the seed will be cheaper. This research aims to get papaya’s seed which can grow fast and uniformly using growth stimulant substance just like gibberellins that is easy to get and cheap. The experiment studies using concentrations of union juice as the substance to soak the planting material seed of papaya before it is planted. The test of durability and germination speed toward 10 seeds on opaque paper in a petri dish germination medium which the diameter is 11,5 cm. The experiment of seeds growth uses completely randomized design (RAL) in a factor of water treatment, the concentration of union juice is 5, 10, 15, and 20 ml l-1, and 10 ppm gibberellin. Every treatment (experimental unit) consists of 8 seeds planted on the traypot (combination of plastic pots, which each size about: on the surface and high 5,5x5, 5x5,5 cm, pots base 2,5x2,5 cm, an each traypot consists of 32 pots). Thus, there were 8 unit experiments for each traypot, and every single unit of experiments is repeated 3 times. Traypot and petri dish are put beneath the roof in the terrace protected by paranet 65% with 2,5x1,5x2,0 m (length, width, and height) in size. The union juice which is potential is used as the substitute of plant growth regulators gibberellins in papaya seedling. The response of papaya seed toward union juice with 15 ml l-1 concentration is equal to 10 ppm gibberellin, occurred in germination (durability and speed of germination) and the seed growth (the root length, the stem diameter, number of leaves, and biomass weight).
Pengaruh Pemberian Air Kelapa dan Tipe Sambungan terhadap Keberhasilan Sambung Pucuk Durian (Durio zibethinus M.)
Authors: Yanti Ika Trisna , Sulandjari Sulandjari , Yuniastuti Endang
Published: November 2019

Durian is a tropical native fruit from Indonesia with a distinctive flavor. Durian can be propagated durian generatively and vegetatively. Generative propagation of durian can be done by germinating seeds, which is vegetative propagation can be done by top grafting. Top grafting can be accelerated by the addition of plant growth regulators. Growth regulating substances in coconut water complex compounds are useful in differentiating cells. This study aimed to determine the effect of various concentrations of coconut water and the best type of grafting for the success of durian tops grafting. In this study was conducted in horticultural breeding centers Ranukitri Mojogedang Karanganyar April to September 2012. This research used Completely Randomized Design (CRD), which consisted of 2 types of treatment that top grafting, namely: cleft grafting, slash grafting, side grafting and coconut water concentration with 5 levels: 0%, 25%, 50%, 75%, 100% with 5 replications, so that the total treatment was 75. The results showed that the type of cleft grafting was the best grafting type to be used for the success of top grafting compared with other grafting types. Treating coconut water with a concentration of 25% was able to optimize the growth of durian top grafting than other concentrations.
Peningkatan Infeksi Patogen Busuk Pangkal pada Bawang Putih oleh Meloidogyne dengan Variasi Kerapatan Inokulum
Authors: Wiyono Hadi , Subagya Subagya , Pujiastuti Novi
Published: November 2019

Fusarium oxysporum f. sp.cepae (FOCe) causing basal rot of garlic is the main obstacle in improving garlic production. The presence of root knot nematode (Meloidogynespp.) is thought to cause an increase in the disease was caused by FOCe. This research aimed to study the effect of the presence of Meloidogyne in increasing the disease severity of basal rot of garlic caused by FOCe and interactions between them. The research was conducted through surveys and experimental procedures. The survey was conducted to obtain preliminary data on the relationship between the disease severity of basal rot of garlic and the infection of Meloidogyne in soil. Experimental study were prepared using a complete randomized block design, with two factors consisting of three levels inoculum density. The first factor was FOCe and the second factor was Meloidogyne spp. The results showed that the presence of Meloidogyne could increase the disease severity of basal rot of garlic
Pertumbuhan Tomat pada Frekuensi Pengairan yang Berbeda
Authors: Jumawati Riana , Sakya Amalia Tetrani , Rahayu Muji
Published: November 2019

Tomato (Lycopersicum esculentum Mill) is one of horticulture commodities with high economic value and still need seriously handling especially for increasing fruits quality and quantity. However the production of tomato has not fullfilleed the demand yet. Therefore it is necessary to develop production. On lowland, the issue not only high temperature but also availability of water is unpredictable.This study aimed to investigate the effect of irrigation frequency on the growth of Mutiara, Opal, and Gondol tomato variety. This research was conducted at the Greenhouse Laboratory of the Agriculture Faculty, Sebelas Maret University with attitude 95 m asl. Implementation of the research conducted from January to May 2013. This research used completely randomized factorial design with two factors, namely variety of tomato (Gondol, Opal and Mutiara) and watering frequencies (everyday, once in 3 days, once in 6 days, and once in 9 days). The results showed that plant height, number of leaves and number of branches are influenced by the variety of tomato and irrigation frequency, whereas chorophyl content is influenced by the watering frequency. Growth component and chlorophyll content of tomato decreased with increasing irrigation frequency.
Pemetaan Status Kerusakan Tanah di Lahan Pertanian di Kecamatan Cepogo Kabupaten Boyolali
Authors: Sumarno Sumarno , Hartati Sri , Hapsari Ratna Cahyaning
Published: November 2019

Land used in agriculture plays an important role in supporting the production of biomass. Destruction of land for biomass production is changing the nature of the soil that exceeded the standard criteria of soil damage. Mapping of land degradation status is useful to know the condition of the soil and tillage correct action. This study was conducted using a survey in the Subdistrict Cepogo Regency Boyolali. Existing data will be scoring and macthing with standard criteria for biomass production ground damage that Government Regulation No. 150 of 2000. Analysis of the data shows that there is no damage land area covering 744,1 Ha (13,7%) and there are areas relatively lightly damaged by parameters of degradation status, namely the composition of the fraction covering 494,4 ha (9,1%), the degree of release of water parameters covering 294,4 ha (5,4%) and a combination of parameters fraction composition and the degree of release of water covering 1.813 Ha (33, 4%).
Aktivitas Antifeedant dan Antioviposisi Ekstrak Daun Tithonia terhadap Kutu Kebul
Authors: Susanti Dian , Widyastuti Rahma , Sulistyo Ato
Published: November 2019

The increasing demand of jamu/traditional medicine was followed by the demands of raw materials quality based on Good Agricultural and Collection Practices (GACP) yield and standard quality. Bio-insecticide is an effort to fulfill the standard based on GACP. Indonesian’s biodiversity have many potential raw materials of bio-insecticides, such as Tithonia diversifolia. The most common pest in the medicinal plants is Aleurodicus dugesii (giant whiteflies), this insect belong to Hemiptera ordo and Aleyrodidae family. The research was conducted to determine the antifeedant and anti-oviposition activity of Tithonia bio-insecticide against giant whitefly on Coleus species. The experimental design was completely randomized design (CRD) with five treatments of Tithonia leaves extract consentrations. Those consentrations were 0, 4, 2, 1, and 0,5 mg L-1. The treatments were tested by using a no-choice test with three replications. The extract of Tithonia leaves was prepared by infundation method by using water solvent. Results showed that extracts of Tithonia with the smallest concentration (0,5 mg L-1) has an active role as an anti-oviposition and antifeedant on giant whiteflies.
Perbandingan Komposisi Ukuran Serat Batang Aren dengan Pasir Sebagai Substrat Hidroponik Seladaarenga wood fiber composition; sand; fiber size; lettuce; hydroponic substrate
Authors: Hardjoko Dwi , Sumiyati Sumiyati
Published: November 2019

Lettuce (Lactuca sativa L.) is vegetables with high nutrient content and interest more people, meanwhile producing optimum, yet, therefore, need hydroponic substrate to increase productivity. The orange wood fiber is a waste produced by palm industry manufacturer. The waste of Arenga wood fibers has not used maximum yet, so if it’s piled up will be affects the ecosystem in around. We need to utilize the waste of Arenga wood fiber to be a substrate hydroponic. The aim of this research is to know the maximum of Arenga wood fiber composition in several sizes which is combined with the sands. It’s for increasing the growth and lettuce yield. The method of this research is completely randomized design with two factorials. They are the Arenga wood fiber composition with sand and size of Arenga wood fiber. The main variable of observation is root length, wide of leaves, and fresh weight of lettuce. The result of observation showed that the Arenga wood fiber composition and the sand composition for cultivation lettuce consist of 25% of Arenga wood fiber and 75% (1:3) of various size of sand. The highest root was 12.6 cm and it produced wide of leaves was 1602.3 cm2 and increased the fresh plants until 53.7 g.
Respon Kunir Putih (Kaempferia rotunda) terhadap Pemberian IBA dan BAP pada Kultur In Vitro
Authors: Yunus Ahmad , Rahayu Muji , Samanhudi Samanhudi , Pujiasmanto Bambang , Riswanda Himawan Joko
Published: November 2019

Kaempferia rotunda that long ago been used to treat gastrointestinal disorders and also treat cancer, has promising opportunities to cultivate. There are several obstacles in cultivation today. Plant propagation by in vitro can be one solution. Basic media and plant growth promoting substance usage is an important factor which has to be concerned. Research aiming to produce high quality seed and in large numbers. Research has been carried out in Tissue Culture Laboratory, Agriculture Faculty, Sebelas Maret University, Surakarta, during July 2014 to August 2015. This research was used MS (Murashige and Skoog) media with combination treatment of each IBA and BAP concentration of 0, 1, 2, 3, and 4 ppm. The number of shoots that appear on the explant is the main observation variables. The results showed IBA and BAP are likely to affect the appearance of buds as much as 2 pieces in each explant at 0 ppm IBA treatment.
Pemberian Mulsa dan Penguat Teras Pada Tiga Jenis Tanaman Terhadap Limpasan Permukaan, Erosi, Pertumbuhan dan Hasil Tanaman Pada Tanah Andisol
Authors: Suyana Jaka , Sumarno Sumarno , Supriyono Supriyono , Lestariningsih Nanik Puji
Published: November 2019

Erosion is the main factor causing  land degradation in area of planting vegetables dominated by Andisol soil. Andisol soil contains more fraction of dust so it is easily transported by runoff. This study aim to determine the influence of mulching corn stem with a terrace strengthen (Vetiveria zizanioides, Coix  lacryma-jobi  L., Panicum muticum, Annona squamosa) to surface runoff, erosion, growth and result of plant (red lentils, cabbage and white lentils) in Andisol soil. This study uses a randomized complete block design with four (4) treatment and repeated three (3) times as a group so that there are twelve (12) experimental unit. The grouping based on kinds of plant that are cabbage, red lentils and white lentils. The results showed that MS4 treatment (mulch corn stem twelve (12) ton/ha with panicum muticum+ Annona squamosa as terrace strengthen) produces smallest of surface run off, erosion and dry weight of weeds, increased growth and canopy and increased crop yields 40.27% of red lentils, 57.10% of cabbage and 14.54% of white lentils.
Analisis Pertumbuhan Garut (Marantha arundinaceae) Pada Beberapa Tingkat Naungan
Authors: Supriyono Supriyono , Arni Putri Retno Bandriyati , Wijayanti Riska
Published: November 2019

Arrowroot is a plant that potential to be one of the alternative food source for the community. The intensity of the light received by the plant is one of the influential factors important for plant growth. This study was conducted to determine the arrowroot plant growth analysis at different light. This research was conducted at the Experimental Farm Faculty of Agriculture, University of Sebelas Maret in Jumantono Karanganyar. This study uses a randomized block design Complete (RAKL) one treatment factor with four levels ie without shade, 31% shade, 51% shade and 73% shade. Data were analyzed by analysis of variance and if there is a significant difference continued with DMRT (Duncan Multiple Range Test) level of 5%. The results showed that the treatment of 51% shade known to give better growth results in some growth variables observed were plant height, leaf area index (LAI), segment length and chlorophyll contents.
PENGADAAN KOLEKSI BAHAN PUSTAKA TERCETAK UPT PERPUSTAKAAN UNIVERSITAS JANABADRA YOGYAKARTA
Authors: Maghfiroh Lisa Noviani
Published: November 2019

This study aims to explain the implementation of procurement collection library materials printed on central library Janabadra Yogyakarta, this study used qualitative methods by data collection in the form of observation and interview. Based on procurement activities conducted that the library Janabadra Yogyakarta apply method 2 that is 1. Procurement through the purchase with the publisher a year 2 times. 2 procurement of library materials through gifts / donations from internal and external parties. In conducting library procurement activities in collaboration with lecturers and students in fulfilling the collection in the library that remains based on the development of science and the applicable syllabus. Recommendations from the authors to the library make written policy as the basis of procurement activities and procurement methods are added so that the available collection more diverse.

Keywords : Acquition, Janabadra Library, Librarian
URGENSI KEMAMPUAN KOMUNIKASI INTERPERSONAL YANG BAIK ANTARA STAKEHOLDER DAN PUSTAKAWAN DI PERPUSTAKAAN UMUM
Authors: Bidayasari Siti
Published: November 2019

In the library communication activities are very important activities for librarians. Librarians are the spearhead in the service in the library. The research method used is the literature study model, for the data retrieval instrument the author uses a review or document review then the data obtained will be analyzed using a qualitative descriptive approach. The results showed interpersonal communication skills between stakeholders and librarians in the public library was important because with the
establishment of good and effective interpersonal communication, the relationship was developed, a sense of mutual need, and good cooperation was established, so that there was no more awkwardness, fear, laziness, and the gap between the head of the library and the librarian because the library head and librarian should be united and collaborate in developing the library even better

Keywords: Interpersonal Communication, Stakeholders, Public Library
PROSES EVALUASI KOLEKSI YANG DILAKUKAN DI PERPUSTAKAAN SMA MUHAMMADIYAH 4 YOGYAKARTA
Authors: Rahmawati Irzalina
Published: November 2019

ABSTRACTThe purpose of this study was to determine the collection evaluation process carried out in the Senior High School of Muhammadiyah 4 Yogyakarta library with the benefits of research, that is as a matter of consideration in conducting evaluations in the development of collections carried out at the library so that the collections are in accordance with user needs. The research method used is the method of observation, interviews, and documentation. Based on observations, interviews, and documentation with librarian at Senior High School of Muhammadiyah 4 Yogyakarta, we can conclude the following results: Library evaluation in the Senior High School of Muhammadiyah 4 Yogyakarta includes 4 processes that is collecting all collections of data, check the list of standards such as catalogs and bibliographies, gathering opinions from users who normally come to the library, and direct collection checks.
SENI KOMUNIKASI SEBAGAI BEKAL PUSTAKAWAN DALAM PRESENTASI
Authors: Mustofa Mustofa , Astuti Sundari Juni
Published: November 2019

Arts of Communication as a Librarian in Presentation. Writing this article has a purpose, namely to give knowledge to librarians in order to be able to make presentations well with the art of communication and have a communication style. The art of communication is influenced by the style of communication, style is the characteristic of a person in communication, there are two aspects that can be seen in the style of communication, namely language and rhetoric. The art of effective communication is to use the 7 C model, namely: completeness, conciseness, conciderration, clarity, concreteness, courtesy, correctness. In order for a librarian’s presentation to be successful, there needs to be tips to do, namely: 1) Know PAL well (purpose, audience, logistics), 2) Pay attention to time, 3) Stay relevant, 4) Play emotional buttons, 5) Create notes easy to do.