bluuebunny/crossref_metadata_2025 huggingface dataset:
Quantification of Citrullinated Histone H3 Bound DNA for Detection of Neutrophil Extracellular Traps
Authors: Li Marina , Lin Cindy , Leso Aubrey , Nefedova Yulia
Published: November 2020

Formation of neutrophil extracellular traps (NETs) has been associated with multiple pathologies including cancer. While the visualization of NETs by microscopy is a routine technique, their quantification presents a number of challenges. Commonly, as citrullination of histone H3 is required for NET formation, the presence of this modified histone along with DNA is considered to be a hallmark of NETs. Here, we describe and validate a novel assay for the quantification of NETs based on the detection of citrullinated histone H3 bound to DNA (CitH3DNA binding assay). Using this assay, we investigated the effect of phorbol 12-myristate 13-acetate (PMA) on NET formation by neutrophils isolated from the bone marrow of control and myeloma-bearing mice. We found that PMA induced citrullination of histone H3, an increase in the level of CitH3DNA, and NET formation in neutrophils from both tumor-free and myeloma-bearing mice. The levels of CitH3DNA in the NET fractions, as measured by our assay directly correlated with the citrullination of histone H3 in neutrophils, as detected by Western blotting, and were significantly higher in PMA-stimulated compared to unstimulated neutrophils. Neutrophils from tumor-bearing mice produced more NETs than those from tumor-free counterparts following stimulation with PMA. The increase in NET production correlated with significantly higher histone H3 citrullination levels and increased measurements of CitH3DNA. Thus, our data indicate that bone marrow neutrophils from myeloma-bearing hosts are prone to NET formation.
New International Association for the Study of Lung Cancer (IASLC) Pathology Committee Grading System for the Prognostic Outcome of Advanced Lung Adenocarcinoma
Authors: Weng Ching-Fu , Huang Chi-Jung , Huang Shih-Hung , Wu Mei-Hsuan , Tseng Ailun Heather , Sung Yung-Chuan , Lee Henry Hsin-Chung , Ling Thai-Yen
Published: November 2020

The impact of the new International Association for the Study of Lung Cancer pathology committee grading system for advanced lung adenocarcinoma (LADC) on survival is unclear, especially in Asian populations. In this study, we reviewed the prognostic outcomes of patients with late-stage disease according to the new grading system. We reviewed 136 LADC cases who underwent a small biopsy from 2007 to 2018. Tumors were classified according to the new grading system for LADC. Baseline characteristics (age, sex, smoking status, body mass index, and driver gene mutations) were analyzed. Kaplan–Meier and Cox regression analyses were used to determine correlations with the new grading system and prognosis. Patients with poorly differentiated adenocarcinoma were significantly correlated with a poor progression-free survival (PFS) (p = 0.013) but not overall survival (OS) (p = 0.154). Subgroup analysis showed that wild-type EGFR patients with poorly differentiated adenocarcinoma treated with chemotherapy had significantly worse PFS (p = 0.011). There was no significant difference in survival among the patients with epidermal growth factor receptor mutations who were treated with tyrosine kinase inhibitors. Patients aged >70 years and those with a BMI ≤ 25 kg/m2 and wild-type patients had significantly worse OS in both univariate (HR = 1.822, p = 0.006; HR = 2.250, p = 0.004; HR = 1.537, p = 0.046, respectively) and multivariate analyses (HR = 1.984, p = 0.002; HR = 2.383, p = 0.002; HR = 1.632, p = 0.028, respectively). Despite therapy, patients with poorly differentiated tumors still fared worse than those with better differentiated tumors. No differences were found among the EGFR mutations treated with TKI. Our findings highlight that the therapeutic regimen should be adjusted for EGFR Wild-type patients with poorly differentiated adenocarcinoma treated with chemotherapy to provide better outcomes.
Crystal Structure and Supramolecular Architecture of Antiallergic Diphenylene Diethyl Dioxalamates
Authors: González-González Juan , Magaña-Vergara Nancy , García-Báez Efrén , Padilla-Martínez Itzia , Mojica-Sánchez Juan , Martínez-Martínez Francisco
Published: November 2020

The crystal structure and the supramolecular architectures of the antiallergic compounds N,N′-(4,4′-methanediyl-di-phenyl)-bis-diethyl dioxalamate (1); N′,N′-(4,4′-oxydi-p-phenylene)-bis-diethyl dioxalamate (2); N,N′-(4,4′-biphenylene)-bis- diethyl dioxalamate (3) are reported. The supramolecular self-assembly in 1-3 is driven by N-H···O=C hydrogen bonds and reinforced by C-H···O=C, C-H···π and C=O···C=O interactions. The three compounds preferred to form cross-linked supramolecular architectures. Intermolecular interactions also were studied by the Hirshfeld surface analysis, revealing that the H···H, O···H, and C···H are the more dominant contacts in the three compounds. The knowledge of crystal structure will allow us to perform theoretical studies to evaluate the antiallergic activity of compounds 1-3.
Virtual Sensors for Estimating District Heating Energy Consumption under Sensor Absences in a Residential Building
Authors: Yoon Sungmin , Choi Youngwoong , Koo Jabeom , Hong Yejin , Kim Ryunhee , Kim Joowook
Published: November 2020

District heating (DH) is an energy efficient building heating system that entails low primary energy consumption and reduced environmental impact. The estimation of the required heating load provides information for operators to control district heating systems (DHSs) efficiently. It also yields historical datasets for intelligent management applications. Based on the existing virtual sensor capabilities to estimate physical variables, performance, etc., and to detect the anomaly detection in building energy systems, this paper proposes a virtual sensor-based method for the estimation of DH energy consumption in a residential building. Practical issues, including sensor absences and limited datasets corresponding to actual buildings, were also analyzed to improve the applicability of virtual sensors in a building. According to certain virtual sensor development processes, model-driven, data-driven, and grey-box virtual sensors were developed and compared in a case study. The grey-box virtual sensor surpassed the capabilities of the other virtual sensors, particularly for operation patterns corresponding to low heating, which were different from those in the training dataset; notably, a 16% improvement was observed in the accuracy exhibited by the grey-box virtual sensor, as compared to that of the data-driven virtual sensor. The former sensor accounted for a significantly wider DHS operation range by overcoming training data dependency when estimating the actual DH energy consumption. Finally, the proposed virtual sensors can be applied for continuous commissioning, monitoring, and fault detection in the building, since they are developed based on the DH variables at the demand side.
Potential of an Automated- and Image-Based Cell Counter to Accelerate Microalgal Research and Applications
Authors: Takahashi Toshiyuki
Published: November 2020

Efforts to achieve Sustainable Development Goals (SDGs) have resulted in enhancement of the position of microalgae in feedstocks for food, feed, healthcare, and biofuels. However, stabile microalgal biorefineries require a sustainable and reliable management system of microalgae, which are sensitive to environmental changes. To expand microalgal applicability, assessment and maintenance of microalgal quality are crucial. Compared with conventional methods, including hemocytometry and turbidity, an automated- and image-based cell counter contributes to the establishment of routine management of microalgae with reduced work burden. This review presents the principle of an automated cell counter and highlights the functional capacities of the device for microalgal management. The method utilizing fluorescence function to evaluate the chlorophyll integrity of microalgae may lay the groundwork for making a large variety of microalgal biorefineries, creating an important step toward achieving SDGs.
Probabilistic Quantification in the Analysis of Flood Risks in Cross-Border Areas of Poland and Germany
Authors: Kuźmiński Łukasz , Nadolny Michał , Wojtaszek Henryk
Published: November 2020

Measuring the probability of flood risk is a key issue in the economics of natural disasters. This discipline studies actual and potential effects of natural disasters on the functioning of economic systems. In traditional economic understanding, it is assumed that both the decision making processes and market processes operate within a certain level of access to information. It is also assumed that the effects of certain phenomena are predictable. However, a natural disaster is difficult to predict. It is hard to predict the time of its occurrence, its impact, direct exposure to its effects and finally, its social and economic results. Exposure to a random hazard, combined with the amount of damage resulting from its potential materialization, is called risk. In this study, the authors focus on presenting a method for quantification of the random element of flood risk. We are using measurement data for cross-border areas between Poland and Germany who witnessed a flood of the century in the 1990s. The empirical data illustrate the usefulness and universality of probabilistic quantification methods for flood risk analysis. The analysis of water level is interesting in a much broader context than the hydrological-economic one. In Central Europe, river water level is immediately connected with two other disaster-like phenomena: drought and heavy rainfall. Also, the course of the Oder river is typical for North European Plain. Therefore, the conclusions presented by the authors are universal by nature and describe certain broader phenomena. Employment of methods of probabilistic quantification using extreme values yields very interesting results: flood risk changes dynamically. Five-year period measurements themselves indicate that there are periods of relatively low exposure of the areas to the disaster (with negligible probability 0.02) and periods of disproportionately high risk increase. The risk of exceeding alarm levels and warning levels changes rapidly, reaching as much as 30% in some locations.
Development of a Beta-Type Moderate-Temperature-Differential Stirling Engine Based on Computational and Experimental Methods
Authors: Cheng Chin-Hsiang , Huang Jhen-Syuan
Published: November 2020

Stirling engine is a favorable technique in the application of waste heat recovery or cogeneration system. This paper aims at developing a beta-type Stirling engine which is operated at moderate heating temperature (773–973 K). Rhombic drive mechanism is utilized to make coaxial motion of displacer and piston. Based on the proposed dimensions, a theoretical model combining thermodynamic and dynamic analysis is built to predict the performance of the Stirling engine. Thermodynamic analysis deals with variations of properties in each chamber while dynamic analysis handles the resultant shaft torque produced by the Stirling engine. Furthermore, a prototype engine is manufactured, and experimental test is carried out to validate the simulated results in this research. Under heating temperature of 973 K, charged pressure of 8 bar, rotation speed of 1944 rpm, shaft power of 68 W is obtained from the prototype Stirling engine. Power density is calculated to be 1.889 W-c.c.−1 by theoretical prediction and 1.725 W-c.c.−1 by tested result. The impact of the geometrical dimensions is investigated to survey the optimal piston diameter which is related to compression ratio and swept volume.
The Short-Term Impact of Different Silvicultural Thinnings on Soil Nematode and Microarthropod Biodiversity in Artificial Black Pine Stands
Authors: Landi Silvia , d’Errico Giada , Binazzi Francesco , Di Salvatore Umberto , Gardin Lorenzo , Marchi Maurizio , Mazza Giuseppe , Roversi Pio Federico , Simoncini Stefania , Torrini Giulia , Cantiani Paolo
Published: November 2020

Soil invertebrates represent almost a quarter of the total diversity of living organisms and their activity affects the entire soil ecological process. The choice of adequate thinning systems may differently affect soil nematode and microarthropod biodiversity in artificial black pinewoods. In this work, the results of the impact of different thinnings on the structure of nematode and microarthropod communities was reported. In a short-term experiment, thinning from below and selective thinning were compared to unmanaged stands to provide indications at the regional scale in central Italy. Soil nematode and microarthropod biodiversity was explored by examining community structure, assessing biodiversity. The interaction between environmental variables (crown volume, Photosynthetically Active Radiation, soil texture, soil temperature, and moisture) with taxa abundance of nematodes and microarthropods were also reported. The results indicated that the effects of thinning practices were temporary and varied between years. Soil nematode community shifted during the first and third years of thinning managements only in the Pratomagno site, while soil microarthropod community shifted in both sites only in the second year. The total nematode abundance was minimally affected by thinning practices, while the nematode community composition showed a decrease of omnivores and predators in the first years. Soil indicators showed inconsistent results. In microarthropods, mites and collembola were the least affected by thinning in terms of abundance and species biodiversity, while eu-edaphic taxa of Chilopoda, Diplopoda, and Pauropoda were not influenced by thinning, hemi-edaphic and epi-edaphic taxa of Coleoptera, Diptera, Hymenoptera, Tysanoptera, and Hemiptera were negatively affected. Soil indicators such as Shannon-Weiner and Simpson indices and soil biological quality (QBS-ar) improved in thinning from below in both sites. Soil temperature and moisture were the main driving factors in affecting soil nematode and microarthropods communities. Thinning from below probably allowed a more rapid recovery than selective thinning.
The Impact of COVID-19 on the Management of European Protected Areas and Policy Implications
Authors: McGinlay James , Gkoumas Vassilis , Holtvoeth Jens , Fuertes Ruymán Federico Armas , Bazhenova Elena , Benzoni Alessandro , Botsch Kerstin , Martel Carmen Cabrera , Sánchez Cati Carrillo , Cervera Isabel , Chaminade Guillermo , Doerstel Juliana , García Concepción J. Fagundo , Jones Angela , Lammertz Michael , Lotman Kaja , Odar Majda , Pastor Teresa , Ritchie Carol , Santi Stefano , Smolej Mojca , Rico Francisco Soriano , Waterman Holly , Zwijacz-Kozica Tomasz , Kontoleon Andreas , Dimitrakopoulos Panayiotis G. , Jones Nikoleta
Published: November 2020

The COVID-19 pandemic led to many European countries imposing lockdown measures and limiting people’s movement during spring 2020. During the summer 2020, these strict lockdown measures were gradually lifted while in autumn 2020, local restrictions started to be re-introduced as a second wave emerged. After initial restrictions on visitors accessing many Nature Protected Areas (PAs) in Europe, management authorities have had to introduce measures so that all users can safely visit these protected landscapes. In this paper, we examine the challenges that emerged due to COVID-19 for PAs and their deeper causes. By considering the impact on and response of 14 popular European National and Nature Parks, we propose tentative longer-term solutions going beyond the current short-term measures that have been implemented. The most important challenges identified in our study were overcrowding, a new profile of visitors, problematic behavior, and conflicts between different user groups. A number of new measures have been introduced to tackle these challenges including information campaigns, traffic management, and establishing one-way systems on trail paths. However, measures to safeguard public health are often in conflict with other PA management measures aiming to minimize disturbance of wildlife and ecosystems. We highlight three areas in which management of PAs can learn from the experience of this pandemic: managing visitor numbers in order to avoid overcrowding through careful spatial planning, introducing educational campaigns, particularly targeting a new profile of visitors, and promoting sustainable tourism models, which do not rely on large visitor numbers.
Genomic and Transcriptomic Analysis for Identification of Genes and Interlinked Pathways Mediating Artemisinin Resistance in Leishmania donovani
Authors: Ghosh Sushmita , Verma Aditya , Kumar Vinay , Pradhan Dibyabhaba , Selvapandiyan Angamuthu , Salotra Poonam , Singh Ruchi
Published: November 2020

Current therapy for visceral leishmaniasis (VL), compromised by drug resistance, toxicity, and high cost, demands for more effective, safer, and low-cost drugs. Artemisinin has been found to be an effectual drug alternative in experimental models of leishmaniasis. Comparative genome and transcriptome analysis of in vitro-adapted artesunate-resistant (K133AS-R) and -sensitive wild-type (K133WT) Leishmania donovani parasites was carried out using next-generation sequencing and single-color DNA microarray technology, respectively, to identify genes and interlinked pathways contributing to drug resistance. Whole-genome sequence analysis of K133WT vs. K133AS-R parasites revealed substantial variation among the two and identified 240 single nucleotide polymorphisms (SNPs), 237 insertion deletions (InDels), 616 copy number variations (CNVs) (377 deletions and 239 duplications), and trisomy of chromosome 12 in K133AS-R parasites. Transcriptome analysis revealed differential expression of 208 genes (fold change ≥ 2) in K133AS-R parasites. Functional categorization and analysis of modulated genes of interlinked pathways pointed out plausible adaptations in K133AS-R parasites, such as (i) a dependency on lipid and amino acid metabolism for generating energy, (ii) reduced DNA and protein synthesis leading to parasites in the quiescence state, and (iii) active drug efflux. The upregulated expression of cathepsin-L like protease, amastin-like surface protein, and amino acid transporter and downregulated expression of the gene encoding ABCG2, pteridine receptor, adenylatecyclase-type receptor, phosphoaceylglucosamine mutase, and certain hypothetical proteins are concordant with genomic alterations suggesting their potential role in drug resistance. The study provided an understanding of the molecular basis linked to artemisinin resistance in Leishmania parasites, which may be advantageous for safeguarding this drug for future use.
Has the Health Insurance Coverage of Scaling Contributed Positively to Periodontal Health in Korea?
Authors: Choi Jin-Sun
Published: November 2020

This study aimed to evaluate the effectiveness of the health insurance coverage of dental scaling (introduced in 2013) using the Community Periodontal Index of Treatment Needs parameter among Korean adults aged 20 years or older. We used the Korea National Health and Nutrition Examination Survey data from before and after 2013 to analyze the statistical significance and associations of the covariates with the prevalence of healthy periodontal tissues, prevalence of people in need of scaling, and prevalence of periodontal diseases. The results showed that the prevalence of healthy periodontal tissues increased by 4.9% (from 34.2% to 39.1%), the number of people in need of scaling decreased by 5% (from 65.9% to 60.9%), and the prevalence of periodontal diseases increased by 7.2% (from 23.4% to 30.6%). Moreover, after the scaling coverage policy, the odds ratio of the prevalence of healthy periodontal tissues was 1.10 times higher, the prevalence of the need for scaling was 1.5 times higher, and the prevalence of periodontal diseases was 0.90 times lower. Therefore, the state should formulate policies that provide dental biofilm management through a disclosing agent, impart education about oral hygiene, and develop a health management system that enables the concurrent management of periodontal diseases and systemic diseases.
Key Determinants of Anemia among Youngsters under Five Years in Senegal, Malawi, and Angola
Authors: Khulu Chris , Ramroop Shaun
Published: November 2020

Anemia is characterized as a condition where there is a deficient number of hematocrit, hemoglobin, or red cells in the human body. This condition affects most youngsters under five years old and pregnant women. The fundamental goal of this paper is to investigate anemia, recognize its determinants, and propose critical proposals to achieve 2030 Sustainable Development Goal with a focus on Senegal, Malawi, and Angola. This research utilized 2016 nationally representative information from Senegal, Malawi, and Angola, which involved collecting data on the demographic and health of the populaces. The Demographic and Health Survey information from Senegal, Malawi, and Angola was then merged to create a pooled sample. This statistical technique enables to generalize and compare the results. A generalized linear mixed model was utilized to decide the factors correlated with anemia among youngsters under five years in Senegal, Malawi, and Angola. The analysis was performed in SPSS and SAS software. A generalized linear mixed model results showed that, compared to youngsters aged less than 12 months, youngsters in the age interval 13–23, 24–35, 36–47, and 48–59 months are more likely to be affected by anemia (OR = 1.419, 2.282, 3.174 and 4.874 respectively). In this study, seven factors were included in the final model. However, only five were found to be significant in explaining anemia at the 5% level of significance. The generalized linear mixed model identified youngster’s age, gender, mother’s level of schooling, wealth status, and nutritional status as determinants of anemia among youngsters under five years in Senegal, Malawi, and Angola.
Applicability of the Theory of Planned Behavior for Predicting Alcohol Use in Spanish Early Adolescents
Authors: Cutrín Olalla , Mac Fadden Isotta , Ayers Stephanie L. , Kulis Stephen S. , Gómez-Fraguela Jose Antonio , Marsiglia Flavio F.
Published: November 2020

According to the theory of planned behavior (TPB), intentions to perform a specific behavior are the result of attitudes, norms, and perceived control, and in turn, intentions and perceived control are the main predictors of the behavior. This study aimed to test the applicability of TPB in predicting alcohol use in normative pre-adolescents. The sample was composed of 755 Spanish adolescents aged 11 to 15 (M = 12.24; SD = 0.56), 47.1% females, from 12 state secondary schools in Spain. The results of path analysis indicate that positive attitudes towards alcohol, favorable norms towards alcohol, and offer vulnerability (perceived control) are significantly positively related to intentions to use alcohol as well as negatively related to actual behavioral control (i.e., actual strategies to avoid alcohol use). In turn, intentions to use and actual control predict higher alcohol frequency and heavy drinking. Significant indirect effects of these antecedents were found on alcohol outcomes through the mediation of intentions and actual control. The findings suggest that the validity and applicability of the TPB in normative pre-adolescents depend on the severity of alcohol use and point to a need to consider negative social influence in decision making processes in early adolescence.
The Experience of Emergency Nurses Caring for Patients with Mental Illness: A Qualitative Study
Authors: Chou Hsin-Ju , Tseng Kai-Yu
Published: November 2020

Background: The medical burden of psychiatric disorders continues to increase and has caused a major impact on health, society, human rights, and economy in the world. Patients with mental illness have a higher ratio of emergency department visits than non-psychiatric patients. Psychiatric disorder-related emergency department care is a stress-causing factor in emergency department work. Therefore, the purpose of this study was to explore the experience of emergency department nurses in caring for patients with mental illness. Methods: A descriptive qualitative research design with purposive sampling was adopted. A total of 17 nurses working in the emergency department in central Taiwan were recruited. In-depth semi-structured interviews were conducted and thematic content analysis was performed. Results: Four themes and six sub-themes emerged that described the experiences of emergency nurse caring for patients with mental illness: (1) Mindset; (2) The predicament of psychiatric care: Violence and isolation and helplessness, and lack of therapeutic communication skills; (3) The influence of open space: insufficient safety and privacy; and (4) The educational needs of psychiatric nursing: improving cognition in psychiatric patients and changing negative thinking into positive thinking. Conclusions: The results revealed the experience of emergency nurses in caring for patients with mental illness. Emergency psychiatric nursing training related to foundational psychiatric knowledge, communication skill, concept of recovery, coping with violence restraining are needed for nurses who work in emergency departments.
Hypertensive Disorders of Pregnancy and Medication Use in the 2015 Pelotas (Brazil) Birth Cohort Study
Authors: Leal Lisiane Freitas , Grandi Sonia Marzia , Miranda Vanessa Iribarrem Avena , Dal Pizzol Tatiane da Silva , Platt Robert William , Silveira Mariângela Freitas da , Bertoldi Andréa Dâmaso
Published: November 2020

Hypertensive disorders of pregnancy account for approximately 22% of all maternal deaths in Latin America and the Caribbean. Pharmacotherapies play an important role in preventing and reducing the occurrence of adverse outcomes. However, the patterns of medications used for treating women with hypertensive disorders of pregnancy (HDP) living in this country is unclear. A population-based birth cohort study including 4262 women was conducted to describe the pattern of use of cardiovascular agents and acetylsalicylic acid between women with and without HDP in the 2015 Pelotas (Brazil) Birth Cohort. The prevalence of maternal and perinatal outcomes in this population was also assessed. HDP were classified according to Ministry of Health recommendations. Medications were defined using the Anatomical Therapeutic Chemical Classification System and the substance name. In this cohort, 1336 (31.3%) of women had HDP. Gestational hypertension was present in 636 (47.6%) women, 409 (30.6%) had chronic hypertension, 191 (14.3%) pre-eclampsia, and 89 (6.7%) pre-eclampsia superimposed on chronic hypertension. Approximately 70% of women with HDP reported not using any cardiovascular medications. Methyldopa in monotherapy was the most frequent treatment (16%), regardless of the type of HDP. Omega-3 was the medication most frequently reported by women without HDP. Preterm delivery, caesarean section, low birth weight, and neonatal intensive care admissions were more prevalent in women with HDP. Patterns of use of methyldopa were in-line with the Brazilian guidelines as the first-line therapy for HDP. However, the large number of women with HDP not using medications to manage HDP requires further investigation.
Servant Leadership in a Social Religious Organization: An Analysis of Work Engagement, Authenticity, and Spirituality at Work
Authors: Ortiz-Gómez Mar , Ariza-Montes Antonio , Molina-Sánchez Horacio
Published: November 2020

Religious organizations represent a main part of the third sector and the social economy. Social faith-based institutions have some unique features that, in some respects, differentiate them from other entities, as they are characterized and defined not only by the services they provide, but also by how they provide them. It is part of their mission to convey the values that prevail in their institutional culture while developing their activities, being attractive to those workers who identify with their values. From this point of view, a key element of these entities’ success is that their employees feel identified with their work so that they are engaged in the institution and its values. The style of leadership exercised in such organizations is critical to fostering these attitudes and their long-term survival. This paper aims to study the link between perceived servant leadership by followers and work engagement, as well as the mediating role of authenticity and spirituality at work in this relationship. To this end, 270 workers from a Spanish Catholic organization in the social sector were surveyed. These data were processed by PLS (partial least squares). The results show that a servant leadership style by itself does not directly promote work engagement among employees of the target organization. The engagement of these workers comes through two mediating variables: authenticity and spirituality at work. This study covers a gap in the literature because although there are studies arguing that a strategy of servant leadership is critical to these organizations, to our knowledge, they do not finish demonstrating the fundamental roles that attitudes of authenticity and spirituality at work play in the perception of this type of leadership, achieving greater work engagement.
Attitudes of Spanish Nurses towards Pressure Injury Prevention and Psychometric Characteristics of the Spanish Version of the APuP Instrument
Authors: López-Franco María Dolores , Parra-Anguita Laura , Comino-Sanz Inés María , Pancorbo-Hidalgo Pedro L.
Published: November 2020

The prevention of pressure injuries in hospitalised patients is a critical point of care related to patient safety. Nurses play a key role in pressure injury (PI) prevention, making it important to assess not only their knowledge but also their attitude towards prevention. The main purpose of this study was to translate into Spanish and evaluate the psychometric properties of the Attitude towards Pressure ulcer Prevention instrument (APuP); a secondary aim was to explore the associations of attitude with other factors. A Spanish version was developed through a translation and back-translation procedure. The validation study was conducted on a sample of 438 nursing professionals from four public hospitals in Spain. The analysis includes internal consistency, confirmatory factorial analysis, and construct validity in known groups. The 12-item Spanish version of the APuP fit well in the 5-factor model, with a Cronbach’s alpha of 0.7. The mean APuP score was 39.98, which means a positive attitude. Registered nurses have a slightly better attitude than Assistant nurses. A moderate correlation (R = 0.32) between knowledge and attitude for the prevention of PI was found. As concluded, the Spanish version of the APuP questionnaire is a valid, reliable and useful tool to measure the attitude toward PI prevention in Spanish-speaking contexts. This version has 12 items grouped into 5 factors, and its psychometric properties are similar to those of the original instrument.
Motor Competence and Attainment of Global Physical Activity Guidelines among a Statewide Sample of Preschoolers
Authors: Slaton Anthony , Kowalski Alysse J. , Zemanick Amy , Pulling Kuhn Ann , Hager Erin R. , Black Maureen M.
Published: November 2020

Global physical activity guidelines for preschoolers include 60 min of moderate-to-vigorous physical activity (MVPA) daily. This study, based on the developmental model of motor skill competence, examines how motor competence relates to preschoolers’ likelihood of meeting global guidelines using ankle accelerometry. We measured physical activity using 24-h ankle-placement accelerometry (Actical) for at least two consecutive days (87% with six-seven days), motor competence using the Test of Gross Motor Development-2 (TGMD-2), and BMI-for-age z-scores (BMIz) using anthropometry and age- and sex-specific CDC norms. Caregivers provided demographic characteristics of children’s age, sex, and race. We used multivariable logistic regression to examine how motor competence, BMIz weight status, and demographic characteristics related to meeting global physical activity guidelines. The sample included 588 preschoolers, age 3–5 years; 55% male; 60% white; and 28% overweight/obese; 75% attained the recommended 60 min of MVPA per day. The odds of meeting MVPA guidelines were associated with higher gross motor quotient, higher object control scores, sex (male), age (older), and race (white), but not with BMIz weight status. Findings support the use of 24-h ankle accelerometry among preschoolers and are consistent with the developmental model of motor competence applied to preschoolers, whereby object control competence relates positively to attaining global physical activity guidelines.
Migraine as a Risk Factor for Peripheral Artery Occlusive Disease: A Population-Based Cohort Study
Authors: Kuo Fu-Hsuan , Lee Chia-Yi , Li Ju-Pi , Chung Jui-Fu , Wang Yu-Hsun , Hsieh Ming-Ju , Yang Shun-Fa
Published: November 2020

We aim to evaluate the development of peripheral occlusive artery disease (PAOD) in patients with migraine by using the National Health Insurance Research Database in Taiwan. A retrospective cohort study was conducted and individuals with diagnostic codes of migraine were enrolled in the study group after excluding those diagnosed with PAOD before the index date. Each subject with migraine was propensity-score matched to another non-migraine patient and the latter served as the control group. A total of 37,288 patients were finally enrolled in the groups. The primary outcome was set as the development of PAOD between the two groups while multiple possible risk factors, including demographic data and comorbidities, were analyzed via the Cox proportional hazards regression. There were 885 and 530 PAOD events in the study and control groups, and the study group had a significantly higher adjusted hazard ratio (1.65, 95% confidential interval: 1.48–1.84, p < 0.001), and the cumulative incidence also revealed a correlation between migraine and PAOD. Other potential risk factors related to the existence of PAOD include age, hypertension, chronic kidney disease, diabetes mellitus, coronary artery disease, stroke, and asthma. For individuals without certain systemic diseases including hypertension, chronic liver disease, chronic kidney disease, coronary artery disease, stroke, asthma, or heart failure, the hazard ratio of subsequent PAOD was significantly higher in the migraine patients than that in the non-migraine individuals (all p < 0.001). In conclusion, the presence of migraine is a significant risk factor for the development of subsequent PAOD.
Same Involvement, Different Reasons: How Personality Factors and Organizations Contribute to Heavy Work Investment
Authors: Mazzetti Greta , Guglielmi Dina , Schaufeli Wilmar B.
Published: November 2020

The academic literature has drawn a clear distinction between a positive form (i.e., work engagement) and a negative form (i.e., workaholism) of heavy work investment (HWI). Nevertheless, the different weight of individual and situational factors contributing to their development was not thoroughly explored. This study aims to investigate the role of individual variables (i.e., obsessive–compulsive traits, achievement orientation, perfectionism, and conscientiousness) and situational factors (i.e., job demands and overwork climate) regarding engagement and workaholism simultaneously. Hypotheses were tested using a sample of 523 Italian employees. Results of structural equation modeling revealed that overwork climate and job demands were conversely related to engagement and workaholism, with job demand reporting the strongest association with workaholism. Furthermore, fear of failure was the only individual factor showing a significant and opposite relationship with workaholism and engagement. In contrast, perfectionism was positively associated with both forms of HWI. These results shed light on the potential effectiveness of intervention strategies focused on the employees and organizations in preventing workaholism and promoting engagement.
Preventing Airborne Transmission of SARS-CoV-2 in Hospitals and Nursing Homes
Authors: Ahlawat Ajit , Mishra Sumit Kumar , Birks John W. , Costabile Francesca , Wiedensohler Alfred
Published: November 2020

The first case of the coronavirus disease 2019 (COVID-19), the novel contagious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), was reported in Wuhan, China in December 2019 [...]
Sorting the Healthy Diet Signal from the Social Media Expert Noise: Preliminary Evidence from the Healthy Diet Discourse on Twitter
Authors: Lynn Theo , Rosati Pierangelo , Leoni Santos Guto , Endo Patricia Takako
Published: November 2020

Over 2.8 million people die each year from being overweight or obese, a largely preventable disease. Social media has fundamentally changed the way we communicate, collaborate, consume, and create content. The ease with which content can be shared has resulted in a rapid increase in the number of individuals or organisations that seek to influence opinion and the volume of content that they generate. The nutrition and diet domain is not immune to this phenomenon. Unfortunately, from a public health perspective, many of these ‘influencers’ may be poorly qualified in order to provide nutritional or dietary guidance, and advice given may be without accepted scientific evidence and contrary to public health policy. In this preliminary study, we analyse the ‘healthy diet’ discourse on Twitter. While using a multi-component analytical approach, we analyse more than 1.2 million English language tweets over a 16-month period in order to identify and characterise the influential actors and discover topics of interest in the discourse. Our analysis suggests that the discourse is dominated by non-health professionals. There is widespread use of bots that pollute the discourse and seek to create a false equivalence on the efficacy of a particular nutritional strategy or diet. Topic modelling suggests a significant focus on diet, nutrition, exercise, weight, disease, and quality of life. Public health policy makers and professional nutritionists need to consider what interventions can be taken in order to counteract the influence of non-professional and bad actors on social media.
Impact of Hospital Bed Shortages on the Containment of COVID-19 in Wuhan
Authors: Zhou Weike , Wang Aili , Wang Xia , Cheke Robert A. , Xiao Yanni , Tang Sanyi
Published: November 2020

The global outbreak of COVID-19 has caused worrying concern amongst the public and health authorities. The first and foremost problem that many countries face during the outbreak is a shortage of medical resources. In order to investigate the impact of a shortage of hospital beds on the COVID-19 outbreak, we formulated a piecewise smooth model for describing the limitation of hospital beds. We parameterized the model while using data on the cumulative numbers of confirmed cases, recovered cases, and deaths in Wuhan city from 10 January to 12 April 2020. The results showed that, even with strong prevention and control measures in Wuhan, slowing down the supply rate, reducing the maximum capacity, and delaying the supply time of hospital beds all aggravated the outbreak severity by magnifying the cumulative numbers of confirmed cases and deaths, lengthening the end time of the pandemic, enlarging the value of the effective reproduction number during the outbreak, and postponing the time when the threshold value was reduced to 1. Our results demonstrated that establishment of the Huoshenshan, Leishenshan, and Fangcang shelter hospitals avoided 22,786 people from being infected and saved 6524 lives. Furthermore, the intervention of supplying hospital beds avoided infections in 362,360 people and saved the lives of 274,591 persons. This confirmed that the quick establishment of the Huoshenshan, Leishenshan Hospitals, and Fangcang shelter hospitals, and the designation of other hospitals for COVID-19 patients played important roles in containing the outbreak in Wuhan.
Emotional-Behavioral Regulation, Temperament and Parent–Child Interactions Are Associated with Dopamine Transporter Allelic Polymorphism in Early Childhood: A Pilot Study
Authors: Cimino Silvia , Marzilli Eleonora , Tafà Mimma , Cerniglia Luca
Published: November 2020

International research has highlighted the role played by individual genetic polymorphism, children’s emotional-behavioral functioning, and quality of parent–child feeding interaction in shaping children’s development. Few studies have focused on the dopamine transporter (DAT1) gene in these processes. In a community sample of 81 families with young children aged between 19 and 28 months (37 males and 44 females), this pilot study aimed to explore possible relationships between children’s DAT1 genotype (9/x: 9/9, 9/19 contrasted to 10/10), their own psychological profiles, parental psychopathological risk, and the quality of mother–child and father–child feeding interactions. Children’s DAT1 genotype was assessed collecting DNA through buccal; children’s temperament and emotional-behavioral regulation, and parental psychopathological risk were assessed, respectively, through report-form and self-report instruments; then, dyadic exchanges were videotaped during a mealtime, and coded based on the Scala di Valutazione dell′Interazione Alimentare (SVIA). Results showed significant differences in the variables under study based on children’s DAT1 genotype, with a higher risk associated with the 10/10. Our findings have provided preliminary new evidence on the relationship between a different child’s polymorphisms, their own emotional-behavioral functioning, and the quality of the family environment, with important implications for the planning of more targeted prevention programs.
Medicinal Chemistry Targeting Mitochondria: From New Vehicles and Pharmacophore Groups to Old Drugs with Mitochondrial Activity
Authors: Catalán Mabel , Olmedo Ivonne , Faúndez Jennifer , Jara José A.
Published: November 2020

Interest in tumor cell mitochondria as a pharmacological target has been rekindled in recent years. This attention is due in part to new publications documenting heterogenous characteristics of solid tumors, including anoxic and hypoxic zones that foster cellular populations with differentiating metabolic characteristics. These populations include tumor-initiating or cancer stem cells, which have a strong capacity to adapt to reduced oxygen availability, switching rapidly between glycolysis and oxidative phosphorylation as sources of energy and metabolites. Additionally, this cell subpopulation shows high chemo- and radioresistance and a high capacity for tumor repopulation. Interestingly, it has been shown that inhibiting mitochondrial function in tumor cells affects glycolysis pathways, cell bioenergy, and cell viability. Therefore, mitochondrial inhibition may be a viable strategy for eradicating cancer stem cells. In this context, medicinal chemistry research over the last decade has synthesized and characterized “vehicles” capable of transporting novel or existing pharmacophores to mitochondrial tumor cells, based on mechanisms that exploit the physicochemical properties of the vehicles and the inherent properties of the mitochondria. The pharmacophores, some of which have been isolated from plants and others, which were synthesized in the lab, are diverse in chemical nature. Some of these molecules are active, while others are prodrugs that have been evaluated alone or linked to mitochondria-targeted agents. Finally, researchers have recently described drugs with well-proven safety and efficacy that may exert a mitochondria-specific inhibitory effect in tumor cells through noncanonical mechanisms. The effectiveness of these molecules may be improved by linking them to mitochondrial carrier molecules. These promising pharmacological agents should be evaluated alone and in combination with classic chemotherapeutic drugs in clinical studies.
Human Endometrial Carcinogenesis Is Associated with Significant Reduction in Long Non-Coding RNA, TERRA
Authors: Adishesh Meera , Alnafakh Rafah , Baird Duncan M. , Jones Rhiannon E. , Simon Shannon , Button Lucy , Kamal Areege M. , Kirwan John , DeCruze S. Bridget , Drury Josephine , Saretzki Gabriele , Hapangama Dharani K.
Published: November 2020

Telomeres are transcribed as long non-coding RNAs called TERRAs (Telomeric repeat containing RNA) that participate in a variety of cellular regulatory functions. High telomerase activity (TA) is associated with endometrial cancer (EC). This study aimed to examine the levels of three TERRAs, transcribed at chromosomes 1q-2q-4q-10q-13q-22q, 16p and 20q in healthy (n = 23) and pathological (n = 24) human endometrium and to examine their association with cellular proliferation, TA and telomere lengths. EC samples demonstrated significantly reduced levels of TERRAs for Chromosome 16p (Ch-16p) (p < 0.002) and Chromosome 20q (Ch-20q) (p = 0.0006), when compared with the postmenopausal samples. No significant correlation was found between TERRA levels and TA but both Ch-16p and Ch-20q TERRA levels negatively correlated with the proliferative marker Ki67 (r = −0.35, p = 0.03 and r = −0.42, p = 0.01 respectively). Evaluation of single telomere length analysis (STELA) at XpYp telomeres demonstrated a significant shortening in EC samples when compared with healthy tissues (p = 0.002). We detected TERRAs in healthy human endometrium and observed altered individual TERRA-specific levels in malignant endometrium. The negative correlation of TERRAs with cellular proliferation along with their significant reduction in EC may suggest a role for TERRAs in carcinogenesis and thus future research should explore TERRAs as potential therapeutic targets in EC.
The Potential Role of Hepatocyte Growth Factor in Degenerative Disorders of the Synovial Joint and Spine
Authors: Tonomura Hitoshi , Nagae Masateru , Takatori Ryota , Ishibashi Hidenobu , Itsuji Tomonori , Takahashi Kenji
Published: November 2020

This paper aims to provide a comprehensive review of the changing role of hepatocyte growth factor (HGF) signaling in the healthy and diseased synovial joint and spine. HGF is a multifunctional growth factor that, like its specific receptor c-Met, is widely expressed in several bone and joint tissues. HGF has profound effects on cell survival and proliferation, matrix metabolism, inflammatory response, and neurotrophic action. HGF plays an important role in normal bone and cartilage turnover. Changes in HGF/c-Met have also been linked to pathophysiological changes in degenerative joint diseases, such as osteoarthritis (OA) and intervertebral disc degeneration (IDD). A therapeutic role of HGF has been proposed in the regeneration of osteoarticular tissues. HGF also influences bone remodeling and peripheral nerve activity. Studies aimed at elucidating the changing role of HGF/c-Met signaling in OA and IDD at different pathophysiological stages, and their specific molecular mechanisms are needed. Such studies will contribute to safe and effective HGF/c-Met signaling-based treatments for OA and IDD.