bluuebunny/crossref_metadata_2025 huggingface dataset:
Databases and Bioinformatic Tools for Glycobiology and Glycoproteomics
Authors: Li Xing , Xu Zhijue , Hong Xiaokun , Zhang Yan , Zou Xia
Published: September 2020

Glycosylation plays critical roles in various biological processes and is closely related to diseases. Deciphering the glycocode in diverse cells and tissues offers opportunities to develop new disease biomarkers and more effective recombinant therapeutics. In the past few decades, with the development of glycobiology, glycomics, and glycoproteomics technologies, a large amount of glycoscience data has been generated. Subsequently, a number of glycobiology databases covering glycan structure, the glycosylation sites, the protein scaffolds, and related glycogenes have been developed to store, analyze, and integrate these data. However, these databases and tools are not well known or widely used by the public, including clinicians and other researchers who are not in the field of glycobiology, but are interested in glycoproteins. In this study, the representative databases of glycan structure, glycoprotein, glycan–protein interactions, glycogenes, and the newly developed bioinformatic tools and integrated portal for glycoproteomics are reviewed. We hope this overview could assist readers in searching for information on glycoproteins of interest, and promote further clinical application of glycobiology.
F-Box Gene D5RF Is Regulated by Agrobacterium Virulence Protein VirD5 and Essential for Agrobacterium-Mediated Plant Transformation
Authors: Zhang Shaojuan , Chen Zhuo , Huang Fei , Wang Yafei , Luo Meizhong
Published: September 2020

We previously reported that the Agrobacterium virulence protein VirD5 possesses transcriptional activation activity, binds to a specific DNA element D5RE, and is required for Agrobacterium-mediated stable transformation, but not for transient transformation. However, direct evidence for a role of VirD5 in plant transcriptional regulation has been lacking. In this study, we found that the Arabidopsis gene D5RF (coding for VirD5 response F-box protein, At3G49480) is regulated by VirD5. D5RF has two alternative transcripts of 930 bp and 1594 bp that encode F-box proteins of 309 and 449 amino acids, designated as D5RF.1 and D5RF.2, respectively. D5RF.2 has a N-terminal extension of 140 amino acids compared to D5RF.1, and both of them are located in the plant cell nucleus. The promoter of the D5RF.1 contains two D5RE elements and can be activated by VirD5. The expression of D5RF is downregulated when the host plant is infected with virD5 deleted Agrobacterium. Similar to VirD5, D5RF also affects the stable but not transient transformation efficiency of Agrobacterium. Some pathogen-responsive genes are downregulated in the d5rf mutant. In conclusion, this study further confirmed Agrobacterium VirD5 as the plant transcription activator and identified Arabidopsis thalianaD5RF.1 as the first target gene of VirD5 in regulation.
In Vitro Antagonistic Effect of Gut Bacteriota Isolated from Indigenous Honey Bees and Essential Oils against Paenibacillus Larvae
Authors: Kačániová Miroslava , Terentjeva Margarita , Žiarovská Jana , Kowalczewski Przemysław Łukasz
Published: September 2020

The aim of study was to isolate and identify the gut bacteria of Apis mellifera and to evaluate antagonistic effect of the bacteriota against Paenibacillus larvae, which causes American foulbrood (AFB) in honeybees. The dilution plating method was used for the quantification of selected microbial groups from digestive tract of bees, with an emphasis on the bacteriota of the bees’ intestines. Bacteria were identified using mass spectrometry (MALDI-TOF-MS Biotyper). Overall, five classes, 27 genera and 66 species of bacteria were identified. Genera Lactobacillus (10 species) and Bacillus (8 species) were the most abundant. Gram-negative bacteria were represented with 16 genera, whereas Gram-positive with 10 genera. Delftia acidovorans and Escherichia coli were the most abundant in the digestive tract of honey bee. Resistance to a selection of antimicrobials was assessed for the bacterial isolates from bee gut and confirmed against all antimicrobials included in the study, with the exception of cefepime. Lactobacillus spp., especially L. kunkeei, L. crispatus and L. acidophilus. showed the strongest antimicrobial activity against P. larvae, the causal pathogen of AFB. Antimicrobial activity of essential oils against isolated bacteria and two isolates of P. larvae were assessed. Application of a broad selection of plant essential oils indicated that Thymus vulgaris had the highest antimicrobial activity against P. larvae.
Tumor Infiltrating Regulatory T Cells in Sporadic and Colitis-Associated Colorectal Cancer: The Red Little Riding Hood and the Wolf
Authors: Fantini Massimo Claudio , Favale Agnese , Onali Sara , Facciotti Federica
Published: September 2020

Regulatory T cells represent a class of specialized T lymphocytes that suppress unwanted immune responses and size the activation of the immune system whereby limiting collateral damages in tissues involved by inflammation. In cancer, the accumulation of Tregs is generally associated with poor prognosis. Many lines of evidence indicate that Tregs accumulation in the tumor microenvironment (TME) suppresses the immune response against tumor-associated antigens (TAA), thus promoting tumor progression in non-small cell lung carcinoma (NSLC), breast carcinoma and melanoma. In colorectal cancer (CRC) the effect of Tregs accumulation is debated. Some reports describe the association of high number of Tregs in CRC stroma with a better prognosis while others failed to find any association. These discordant results stem from the heterogeneity of the immune environment generated in CRC in which anticancer immune response may coexists with tumor promoting inflammation. Moreover, different subsets of Tregs have been identified that may exert different effects on cancer progression depending on tumor stage and their location within the tumor mass. Finally, Tregs phenotypic plasticity may be induced by cytokines released in the TME by dysplastic and other tumor-infiltrating cells thus affecting their functional role in the tumor. Here, we reviewed the recent literature about the role of Tregs in CRC and in colitis-associated colorectal cancer (CAC), where inflammation is the main driver of tumor initiation and progression. We tried to explain when and how Tregs can be considered to be the “good” or the “bad” in the colon carcinogenesis process on the basis of the available data concluding that the final effect of Tregs on sporadic CRC and CAC depends on their localization within the tumor, the subtype of Tregs involved and their phenotypic plasticity.
Metabolomics Fingerprint Induced by the Intranigral Inoculation of Exogenous Human Alpha-Synuclein Oligomers in a Rat Model of Parkinson’s Disease
Authors: Murgia Federica , Atzori Luigi , Carboni Ezio , Santoru Maria Laura , Hendren Aran , Pisanu Augusta , Caboni Pierluigi , Boi Laura , Fusco Giuliana , Carta Anna R.
Published: September 2020

Parkinson’s disease (PD) is considered a synucleinopathy because of the intraneuronal accumulation of aggregated α-synuclein (αSyn). Recent evidence points to soluble αSyn-oligomers (αSynO) as the main cytotoxic species responsible for cell death. Given the pivotal role of αSyn in PD, αSyn-based models are crucial for the investigation of toxic mechanisms and the identification of new therapeutic targets in PD. By using a metabolomics approach, we evaluated the metabolic profile of brain and serum samples of rats infused unilaterally with preformed human αSynOs (HαSynOs), or vehicle, into the substantia nigra pars compacta (SNpc). Three months postinfusion, the striatum was dissected for striatal dopamine (DA) measurements via High Pressure Liquid Chromatography (HPLC) analysis and mesencephalon and serum samples were collected for the evaluation of metabolite content via gas chromatography mass spectrometry analysis. Multivariate, univariate and correlation statistics were applied. A 40% decrease of DA content was measured in the HαSynO-infused striatum as compared to the contralateral and the vehicle-infused striata. Decreased levels of dehydroascorbic acid, myo-inositol, and glycine, and increased levels of threonine, were found in the mesencephalon, while increased contents of fructose and mannose, and a decrease in glycine and urea, were found in the serum of HαSynO-infused rats. The significant correlation between DA and metabolite content indicated that metabolic variations reflected the nigrostriatal degeneration. Collectively, the metabolomic fingerprint of HαSynO-infused rats points to an increase of oxidative stress markers, in line with PD neuropathology, and provides hints for potential biomarkers of PD.
Determinants of Social Media Usage in Business by Women: Age and Development of the Country
Authors: Mądra-Sawicka Magdalena , Paliszkiewicz Joanna , Nord Jeretta Horn
Published: September 2020

This paper aims to identify the most important social media purposes of usage by responding women’s attitudes according to age and the economic stage of development of their respective country. Research was done through an online survey in 2017–2018 followed by an analyses of the results from eight countries: four countries that represent an emerging economy and four developed economies. Participants responded to questions concerning social technologies and their purposes of usage as well as resulting job opportunities. The conducted analysis of regarding Facebook as a platform resulted in the highest number of responses in the survey. In this paper, detailed results are presented including a comparative analysis between two groups of economies. Findings reveal that in both groups, the usage of Facebook in business is related mostly to a positive experience. The result showed that among women in emerging economies, social media were used more broadly, and from an age perspective, the results show that marketing is a key benefit emphasized among older respondents. The communication benefit of Facebook usage in business was noticed as a key factor by respondents in groups from both developed and emerging economies.
Bimodal CT/MRI-Based Segmentation Method for Intervertebral Disc Boundary Extraction
Authors: Liaskos Meletios , Savelonas Michalis A. , Asvestas Pantelis A. , Lykissas Marios G. , Matsopoulos George K.
Published: September 2020

Intervertebral disc (IVD) localization and segmentation have triggered intensive research efforts in the medical image analysis community, since IVD abnormalities are strong indicators of various spinal cord-related pathologies. Despite the intensive research efforts to address IVD boundary extraction based on MR images, the potential of bimodal approaches, which benefit from complementary information derived from both magnetic resonance imaging (MRI) and computed tomography (CT), has not yet been fully realized. Furthermore, most existing approaches rely on manual intervention or on learning, although sufficiently large and labelled 3D datasets are not always available. In this light, this work introduces a bimodal segmentation method for vertebrae and IVD boundary extraction, which requires a limited amount of intervention and is not based on learning. The proposed method comprises various image processing and analysis stages, including CT/MRI registration, Otsu-based thresholding and Chan–Vese-based segmentation. The method was applied on 98 expert-annotated pairs of CT and MR spinal cord images with varying slice thicknesses and pixel sizes, which were obtained from 7 patients using different scanners. The experimental results had a Dice similarity coefficient equal to 94.77(%) for CT and 86.26(%) for MRI and a Hausdorff distance equal to 4.4 pixels for CT and 4.5 pixels for MRI. Experimental comparisons with state-of-the-art CT and MRI segmentation methods lead to the conclusion that the proposed method provides a reliable alternative for vertebrae and IVD boundary extraction. Moreover, the segmentation results are utilized to perform a bimodal visualization of the spine, which could potentially aid differential diagnosis with respect to several spine-related pathologies.
Risk Stratification for Patients with Chest Pain Discharged Home from the Emergency Department
Authors: Kavsak Peter A. , Cerasuolo Joshua O. , Mondoux Shawn E. , Sherbino Jonathan , Ma Jinhui , Hoard Brock K. , Perez Richard , Seow Hsien , Ko Dennis T. , Worster Andrew
Published: September 2020

For patients with chest pain who are deemed clinically to be low risk and discharged home from the emergency department (ED), it is unclear whether further laboratory tests can improve risk stratification. Here, we investigated the utility of a clinical chemistry score (CCS), which comprises plasma glucose, the estimated glomerular filtration rate, and high-sensitivity cardiac troponin (I or T) to generate a common score for risk stratification. In a cohort of 14,676 chest pain patients in the province of Ontario, Canada and who were discharged home from the ED (November 2012–February 2013 and April 2013–September 2015) we evaluated the CCS as a risk stratification tool for all-cause mortality, plus hospitalization for myocardial infarction or unstable angina (primary outcome) at 30, 90, and 365 days post-discharge using Cox proportional hazard models. At 30 days the primary outcome occurred in 0.3% of patients with a CCS < 2 (n = 6404), 0.9% of patients with a CCS = 2 (n = 4336), and 2.3% of patients with a CCS > 2 (n = 3936) (p < 0.001). At 90 days, patients with CCS < 2 (median age = 52y (IQR = 46–60), 59.4% female) had an adjusted HR = 0.51 (95% confidence interval (CI) = 0.32–0.82) for the composite outcome and patients with a CCS > 2 (median age = 74y (IQR = 64–82), 48.0% female) had an adjusted HR = 2.80 (95%CI = 1.98–3.97). At 365 days, 1.3%, 3.4%, and 11.1% of patients with a CCS < 2, 2, or >2 respectively, had the composite outcome (p < 0.001). In conclusion, the CCS can risk stratify chest pain patients discharged home from the ED and identifies both low- and high-risk patients who may warrant different medical care.
Perinatal Predictors of Postpartum Depression: Results of a Retrospective Comparative Study
Authors: Banasiewicz Jolanta , Zaręba Kornelia , Bińkowska Małgorzata , Rozenek Hanna , Wójtowicz Stanisław , Jakiel Grzegorz
Published: September 2020

Postpartum mood disorders occur in a considerable number of women with the most common postpartum disorder being baby blues. The study aimed at the identification of the risk factors present before delivery, which may be comprised in prophylactic programs concerning postpartum mood disorders. The research material includes data retrieved from the medical record of patients delivering in Warsaw in the years 2010–2017 who routinely completed Edinburgh Postnatal Depression Scale (EPDS) after delivery. Data of 604 patients were analyzed. The study group included 75 women who obtained at least 12 points in EPDS, which constituted 12.4% of the whole group (mean = 14.92, SD = 3.05). The control group was made up of 75 women who obtained no more than 5 points in EPDS. A significant correlation was reported between the parity and their order vs. the risk of developing postpartum mood disorders. Women with an increased risk delivered at about 37 gestational weeks, while women in whom the risk of such disorders was low delivered at about 39 gestational weeks. No increased risk was noted in women with premature rupture of membranes. Primigravidas and women who delivered prematurely were the most predisposed to developing postpartum depression and should undergo screening tests in the perinatal period.
Long-Term Safety of Endoscopic Biliary Stents for Cholangitis Complicating Choledocholithiasis: A Multi-Center Study
Authors: Sbeit Wisam , Khoury Tawfik , Kadah Anas , M. Livovsky Dan , Nubani Adi , Mari Amir , Goldin Eran , Mahamid Mahmud
Published: September 2020

Background: Treatment of cholangitis complicating choledocholithiasis includes biliary sphincterotomy and stone extraction. In certain cases of elderly comorbid patients with high risk for definitive endoscopic treatment, biliary stenting is the only measure for relieving biliary obstruction. Aim: We aimed to report the safety of retained biliary stone. Methods: a multi-center, retrospective case-control study conducted at two Israeli medical centers from January 2013 to December 2018 including all patients 18 years of age or older who underwent ERCP and biliary stent insertion for the treatment of acute cholangitis due to choledocholithiasis. Results: Three-hundred and eight patients were identified. Eighty-three patients had retained long-term biliary stents of more than 6 months (group A) from insertion compared to 225 patients whose biliary stents were removed within a 6-month period (group B). The mean follow-up in group A was 66.1± 16.3 vs. 11.1 ± 2.7 weeks in group B. Overall complications during the follow-up were similar between groups A and B (6% vs. 4.9%, OR 1.24, Chi square 0.69). Similarly, the rate of each complication alone was not different when comparing group A to group B (3.6%, 1.2% and 1.2% vs. 2.7%, 0.44% and 1.8%) for cholangitis, stent related pancreatitis and biliary colic, respectively (Chi square 0.85). Even after 12 months, the rates of overall complications and each complication alone were not higher compared to less than 12 months (Chi square 0.72 and 0.8, respectively). Conclusion: endoscopic biliary stenting for cholangitis complicating choledocholithiasis is safe for the long-term period without increase in stent related complications.
Analysis of Tuberculosis Meningitis Pathogenesis, Diagnosis, and Treatment
Authors: Arshad Aysha , Dayal Sujay , Gadhe Raj , Mawley Ajinkya , Shin Kevin , Tellez Daniel , Phan Phong , Venketaraman Vishwanath
Published: September 2020

Tuberculosis (TB) is the most prevalent infectious disease in the world. In recent years there has been a significant increase in the incidence of TB due to the emergence of multidrug resistant strains of Mycobacterium tuberculosis (M. tuberculosis) and the increased numbers of highly susceptible immuno-compromised individuals. Central nervous system TB, includes TB meningitis (TBM-the most common presentation), intracranial tuberculomas, and spinal tuberculous arachnoiditis. Individuals with TBM have an initial phase of malaise, headache, fever, or personality change, followed by protracted headache, stroke, meningismus, vomiting, confusion, and focal neurologic findings in two to three weeks. If untreated, mental status deteriorates into stupor or coma. Delay in the treatment of TBM results in, either death or substantial neurological morbidity. This review provides latest developments in the biomedical research on TB meningitis mainly in the areas of host immune responses, pathogenesis, diagnosis, and treatment of this disease.
Proton Pump Inhibitor Use May Increase the Risk of Diverticulitis but Not It’s Severity among Patients with Colonic Diverticulosis: A Multicenter Study
Authors: Sbeit Wisam , Khoury Tawfik , Kadah Anas , Asadi Waseem , Shahin Amir , Lubany Ahmad , Safadi Mohammed , Haddad Haya , Abu Ahmad Ruba , Abu El Hija Sami , Abboud Rand , Mahamid Mahmud , Pellicano Rinaldo , Mari Amir
Published: September 2020

Colonic diverticular disease, especially diverticulitis constitutes a major cause of hospitalization and an economic burden in developed countries. Proton pump inhibitors (PPIs) are among the commonest drugs used to treat several diseases affecting the upper gastrointestinal tract. A few studies have reported that the use of Proton Pump Inhibitors PPIs caused dysbiosis. In this study, we searched for a relationship between PPI use and the onset and severity of diverticulitis in patients with colonic diverticulosis. In a retrospective study, patients who were hospitalized for documented diverticulitis were enrolled as cases and compared with a control group of patients with uncomplicated diverticulosis. Overall, 613 patients who had a diagnosis of diverticulosis were included in the study, 217 of whom had diverticulitis. After multivariate analysis, the non-modifiable risk factors associated with diverticulitis included: age (p < 0.0001), hypertension (p < 0.0001), chronic renal failure (p = 0.007), diabetes mellitus (p < 0.0001), and left colon location (p = 0.02). However, among the modifiable factors, only PPI use (p < 0.0001) showed a significant association. Advanced disease severity (according to Hinchey classification of diverticulitis stages II–IV) was associated with aspirin use (p = 0.0004) and pan-colonic location (p = 0.02). PPI use was the only modifiable factor significantly associated with diverticulitis, but not with its severity, among patients with diverticulosis. This observation should be confirmed in future multicenter prospective studies.
Outcomes of Inhaled Amikacin and Clofazimine-Containing Regimens for Treatment of Refractory Mycobacterium avium Complex Pulmonary Disease
Authors: Kim Bo-Guen , Kim Hojoong , Kwon O. Jung , Huh Hee Jae , Lee Nam Yong , Baek Sun-Young , Sohn Insuk , Jhun Byung Woo
Published: September 2020

Limited data are available regarding optimal treatment for refractory Mycobacterium avium complex-pulmonary disease (MAC-PD). We evaluated outcomes of inhaled amikacin (AMK) with clofazimine (CFZ) regimens as an add-on salvage therapy for refractory MAC-PD. We retrospectively analyzed 52 patients with refractory MAC-PD, characterized by persistently positive sputum cultures despite >6 months of treatment. Thirty-five (67%) patients had M. intracellulare-PD, and 17 (33%) patients had M. avium-PD. Twenty-seven (52%) patients received the salvage therapy for ≥12 months, whereas 25 (48%) patients were treated for <12 months due to adverse effects or other reasons. Seventeen (33%) patients had culture conversion: 10 (10/27) in the ≥12-month treatment group and seven (7/25) in the <12-month treatment group (p = 0.488). Microbiological cure, defined as maintenance of culture negativity, was achieved in 12 (23%) patients; six (6/12) with accompanying symptomatic improvement were considered to have reached cure. Clinical cure, defined as symptomatic improvement with <3 consecutive negative cultures, was achieved in three (6%) patients. Overall, 15 (29%) patients achieved favorable outcomes, including microbiological cure, cure, and clinical cure. Inhaled AMK with CFZ may provide favorable outcomes in some patients with refractory MAC-PD. However, given the adverse effects, more effective strategies are needed to maintain these therapeutic regimens.
Minimally Invasive Intraoral Approach to Submandibular Lodge
Authors: Galli Massimo , Fusconi Massimo , Federici Francesca Romana , Candelori Francesca , De Vincentiis Marco , Polimeni Antonella , Testarelli Luca , Cassese Benedetta , Miccoli Gabriele , Greco Antonio
Published: September 2020

The purpose of this study is to describe the Minimally Invasive Intraoral Approach (MIIA) performed on selected cases of abscesses and neck phlegmons of odontogenic origin when the infection has not spread beyond the inferior mandibular margin. This technique allows us to avoid cervicotomy by a direct approach to the abscess, draining it through the oral cavity. If the limits have already been crossed, then cervicotomy is necessary. The aim of the study is to show the surgical outcomes that we have achieved during a time span of two years, and to show the effectiveness of the MIIA and its results. We selected 66 patients with abscesses and neck phlegmons, from January 2018 to June 2020. Among these cases, five patients were excluded as it was not possible to recover medical records from database. The MIIA technique has been performed on 16 patients (26.2%) when a successful dental extraction and drainage of the submandibular lodge were accomplished. The patients who underwent the MIIA surgery have all perfectly healed and did not suffer from relapses during the follow-up. The results show the achievement of excellent healing, underlining the lower impact required by MIIA when compared to a more traditional approach through cervicotomy.
Mitochondrial DNA Parameters in Blood of Infants Receiving Lopinavir/Ritonavir or Lamivudine Prophylaxis to Prevent Breastfeeding Transmission of HIV-1
Authors: Monnin Audrey , Nagot Nicolas , Periès Marianne , Vallo Roselyne , Meda Nicolas , Singata-Madliki Mandisa , Tumwine James K. , Kankasa Chipepo , Ngandu Nobubelo , Goga Ameena , Reynier Pascal , Tylleskär Thorkild , Van de Perre Philippe , Molès Jean-Pierre
Published: September 2020

Children who are human immunodeficiency virus (HIV)-exposed but uninfected (CHEU) accumulate maternal HIV and antiretroviral exposures through pregnancy, postnatal prophylaxis, and breastfeeding. Here, we compared the dynamics of mitochondrial DNA (mtDNA) parameters in African breastfed CHEU receiving lopinavir/ritonavir (LPV/r) or lamivudine (3TC) pre-exposure prophylaxis during the first year of life. The number of mtDNA copies per cell (MCN) and the proportion of deleted mtDNA (MDD) were assessed at day 7 and at week 50 post-delivery (PrEP group). mtDNA depletion was defined as a 50% or more decrease from the initial value, and mtDNA deletions was the detection of mtDNA molecules with large DNA fragment loss. We also performed a sub-analysis with CHEU who did not receive a prophylactic treatment in South Africa (control group). From day seven to week 50, MCN decreased with a median of 41.7% (interquartile range, IQR: 12.1; 64.4) in the PrEP group. The proportion of children with mtDNA depletion was not significantly different between the two prophylactic regimens. Poisson regressions showed that LPV/r and 3TC were associated with mtDNA depletion (reference: control group; LPV/r: PR = 1.75 (CI95%: 1.15–2.68), p < 0.01; 3TC: PR = 1.54 (CI95%: 1.00–2.37), p = 0.05). Moreover, the proportion of children with MDD was unexpectedly high before randomisation in both groups. Long-term health impacts of these mitochondrial DNA parameters should be investigated further for both CHEU and HIV-infected children receiving LPV/r- or 3TC- based regimens.
A Review of the Incidence Diagnosis and Treatment of Spontaneous Hemorrhage in Patients Treated with Direct Oral Anticoagulants
Authors: Gunasekaran Kulothungan , Rajasurya Venkat , Devasahayam Joe , Singh Rahi Mandeep , Chandran Arul , Elango Kalaimani , Talari Goutham
Published: September 2020

Anticoagulation carries a tremendous therapeutic advantage in reducing morbidity and mortality with venous thromboembolism and atrial fibrillation. For over six decades, traditional anticoagulants like low molecular weight heparin and vitamin K antagonists like warfarin have been used to achieve therapeutic anticoagulation. In the past decade, multiple new direct oral anticoagulants have emerged and been approved for clinical use. Since their introduction, direct oral anticoagulants have changed the landscape of anticoagulants. With increasing indications and use in various patients, they have become the mainstay of treatment in venous thromboembolic diseases. The safety profile of direct oral anticoagulants is better or at least similar to warfarin, but several recent reports are focusing on spontaneous hemorrhages with direct oral anticoagulants. This narrative review aims to summarize the incidence of spontaneous hemorrhage in patients treated with direct oral anticoagulants and also offers practical management strategies for clinicians when patients receiving direct oral anticoagulants present with bleeding complications.
Value of Cardiac Biomarkers in the Early Diagnosis of Takotsubo Syndrome
Authors: Dagrenat Charlotte , Von Hunolstein Jean Jacques , Matsushita Kensuke , Thebaud Lucie , Greciano Stéphane , Tuzin Nicolas , Meyer Nicolas , Trinh Annie , Jesel Laurence , Ohlmann Patrick , Morel Olivier
Published: September 2020

Background: Bedside diagnosis between Takotsubo syndrome (TTS) and ST elevation (STEMI) and non-ST elevation (NSTEMI) myocardial infarction remains challenging. We sought to determine a cardiac biomarker profile to enable their early distinction. Methods: 1100 patients (TTS n = 314, STEMI n = 452, NSTEMI n = 334) were enrolled in two centers. Baseline clinical and biological characteristics were compared between groups. Results: At admission, cut-off values of BNP (B-type natriuretic peptide)/TnI (Troponin I) ratio of 54 and 329 distinguished respectively STEMI from NSTEMI, and NSTEMI from TTS. Best differentiation was obtained by the use of BNP/TnI ratio at peak (cut-of values of 6 and 115 discriminated respectively STEMI from NSTEMI, and NSTEMI from TTS). We developed a score including five parameters (age, gender, history of psychiatric disorders, LVEF, and BNP/TnI ratio at admission) enabling good distinction between TTS and STEMI (77% specificity and 92% sensitivity, AUC 0.93). For the distinction between TTS and NSTEMI, a four variables score (gender, history of psychiatric disorders, LVEF, and BNP at admission) achieved a good diagnostic performance (89% sensitivity, 85% specificity, AUC 0.94). Conclusion: A distinctive cardiac biomarker profile enables at an early stage a differentiation between TTS and ACS. A four (NSTEMI) or five variables score (STEMI) permitted a better discrimination.
Cost-Benefit Analysis of Adaptation to Beach Loss Due to Climate Change in Japan
Authors: Nakajima Kazunori , Sakamoto Naoki , Udo Keiko , Takeda Yuriko , Ohno Eiji , Morisugi Masafumi , Mori Ryuta
Published: September 2020

To measure economic effects of changes in environmental quality caused by climate change in Japan, we estimate beach loss damage costs in Japan and in each prefecture and evaluate the economic effectiveness of hypothetical adaptation measures to restore sandy beaches. For analyses, we use a computable general equilibrium model (CGE) that integrates a utility function with environmental quality factors as an independent variable derived from a recreation demand function in a travel cost method (TCM). We use future projections of beach loss rates in 2081–2100 based on ensemble-mean regional sea-level rise (SLR) for four Representative Concentration Pathway (RCPs) scenarios (RCP2.6, RCP4.5, RCP6.0, and RCP8.5). The main findings of our study are presented as follows. (1) In 2081–2100, beach loss damage costs were estimated respectively as 398.54 million USD per year for RCP2.6, 468.96 (m.USD/year) for RCP4.5, 494.09 (m.USD/year) for RCP6.0, and 654.63 (m.USD/year) for RCP8.5. (2) For all RCPs, six prefectures for which the cost–benefit ratio exceeds 1.0 were Kanagawa, Osaka, Hyogo, Hiroshima, Saga, and Kumamoto. Our hypothetical adaptation measure of an artificial beach enhancement is expected to be quite effective as a public works project in these prefectures.
Soil Organic Matter, Mitigation of and Adaptation to Climate Change in Cocoa–Based Agroforestry Systems
Authors: Gusli Sikstus , Sumeni Sri , Sabodin Riyami , Muqfi Ikram Hadi , Nur Mustakim , Hairiah Kurniatun , Useng Daniel , van Noordwijk Meine
Published: September 2020

Belowground roles of agroforestry in climate change mitigation (C storage) and adaptation (reduced vulnerability to drought) are less obvious than easy-to-measure aspects aboveground. Documentation on these roles is lacking. We quantified the organic C concentration (Corg) and soil physical properties in a mountainous landscape in Sulawesi (Indonesia) for five land cover types: secondary forest (SF), multistrata cocoa–based agroforestry (CAF) aged 4–5 years (CAF4), 10–12 years (CAF10), 17–34 years (CAF17), and multistrata (mixed fruit and timber) agroforest (MAF45) aged 45–68 years. With four replicate plots per cover type, we measured five pools of C-stock according to IPCC guidelines, soil bulk density (BD), macro porosity (MP), hydraulic conductivity (Ks), and available water capacity of the soil (AWC). The highest C-stock, in SF, was around 320 Mg ha−1, the lowest, 74 Mg ha−1, was in CAF4, with the older agroforestry systems being intermediate with 120 to 150 Mg ha−1. Soil compaction after forest conversion led to increased BD and reduced MP, Ks, and AWC. Older agroforestry partly recovered buffering: AWC per m of rooted soil profile increased by 5.7 mm per unit (g kg−1) increase of Corg. The restored AWC can support about a week’s worth of evapotranspiration without rain, assisting in climate change adaptation.
Trees and Crops Arrangement in the Agroforestry System Based on Slope Units to Control Landslide Reactivation on Volcanic Foot Slopes in Java, Indonesia
Authors: Purwaningsih Rina , Sartohadi Junun , Setiawan Muhammad Anggri
Published: September 2020

Agroforestry, as the dominant land use at the volcanic foot slope in Java Island, is prone to landslide due to a combination of rough relief and thick soil layer. However, evaluations of specific vegetation patterns against landslide reactivation due to soil erosion, which relays on the existing slope units and geomorphological processes, are still limited. The research data were collected through aerial photo interpretation by delineating morphological units of old landslides, slope units, and the existing land use. This was followed by field surveys for two consecutive purposes, i.e., (1) verification of aerial photo interpretation and (2) identification and intensity assessment of existing geomorphological processes. The data were tabulated according to slope units, as a basis for tree and crop arrangement in controlling erosion and landslide, by considering economic, social, and ecological functions. The agroforestry would control the landslides reactivation if the tree and crop arrangement was based on the morphological units formed by the previous landslide. The slope units are classified into residual zones at the highest elevations with flat slopes, erosion zones with the steepest slope, and sedimentation zones at the lowest elevations with gentle slopes. Trees and crops at those three units of the former landslide have different functions in controlling processes of rill erosion, gully erosion, and soil creep.
From Bench to Bedside in Tongue Muscle Cancer Invasion and Back again: Gross Anatomy, Microanatomy, Surgical Treatments and Basic Research
Authors: Calabrese Luca , Bizzoca Maria Eleonora , Grigolato Roberto , Maffini Fausto Antonio , Tagliabue Marta , Negro Rosa , Leuci Stefania , Mignogna Michele Davide , Lo Muzio Lorenzo
Published: September 2020

Tongue squamous cell carcinoma is the most common malignancy in the oral cavity. Despite advances in diagnosis and treatment, the prognosis of advanced states has not significantly improved. Depth of invasion, pattern of invasion such as tumor budding grade, lingual lymph node metastasis in early stages, collective cell migration and circulating tumor cells in peripheral blood are some examples of the mechanisms that are currently receiving increasing attention in the evaluation of the prognosis of tongue cancers. Anatomic-based surgery showed that it is possible to improve loco-regional control of tongue cancer. In patients with a “T-N tract involvement”, there is significantly more distant recurrence (40%) in patients undergoing a compartmental tongue surgery. In general, the neoplastic infiltration of the lingual muscles is traced back to the finding of neoplastic tissue along the course of a muscle; however, the muscle fibers, due to their spatial conformation and the organization of the extracellular matrix, could influence the movement of tumor cells through the muscle, leaving its three-dimensional structure unchanged. We need to exclude the possibility that tongue muscle fibers represent a mechanism for the diffusion of cancer cells without muscle invasion.
Molecular Dynamics Study on Mechanical Properties of Interface between Urea-Formaldehyde Resin and Calcium-Silicate-Hydrates
Authors: Wang Xianfeng , Xie Wei , Li Taoran , Ren Jun , Zhu Jihua , Han Ningxu , Xing Feng
Published: September 2020

Microcapsule based self-healing concrete can automatically repair damage and improve the durability of concrete structures, the performance of which depends on the binding behavior between the microcapsule wall and cement matrix. However, conventional experimental methods could not provide detailed information on a microscopic level. In this paper, through molecular dynamics simulation, three composite models of Tobermorite (Tobermorite 9 Å, Tobermorite 11 Å, Tobermorite 14 Å), a mineral similar to Calcium-Silicate–Hydrate (C–S–H) gel, with the linear urea–formaldehyde (UF), the shell of the microcapsule, were established to investigate the mechanical properties and interface binding behaviour of the Tobermorite/UF composite. The results showed that the Young’s modulus, shear modulus and bulk modulus of Tobermorite/UF were lower than that of ‘pure’ Tobermorite, whereas the tensile strength and failure strain of Tobermorite/UF were higher than that of ‘pure’ Tobermorite. Moreover, through radial distribution function (RDF) analysis, the connection between Tobermorite and UF found a strong interaction between Ca, N, and O, whereas Si from Tobermorite and N from UF did not contribute to the interface binding strength. Finally, high binding energy between the Tobermorite and UF was observed. The research results should provide insights into the interface behavior between the microcapsule wall and the cement matrix.
Comparison of Dental Stone Models and Their 3D Printed Acrylic Replicas for the Accuracy and Mechanical Properties
Authors: Czajkowska Marta , Walejewska Ewa , Zadrożny Łukasz , Wieczorek Monika , Święszkowski Wojciech , Wagner Leopold , Mijiritsky Eitan , Markowski Jarosław
Published: September 2020

This study was conducted to test possibilities of application of 3D printed dental models (DMs) in terms of their accuracy and physical properties. In this work, stone models of mandibles were cast from alginate impressions of 10 patients and scanned in order to obtain 3D printed acrylic replicas. The diagnostic value was tested as matching of model scans on three levels: peak of cusps, occlusal surface, and all teeth surfaces. The mechanical properties of acrylic and stone samples, specifically the impact strength, shore D hardness, and flexural and compressive strength were investigated according to ISO standards. The matching of models’ surfaces was the highest on the level of peaks of cusps (average lack of deviations, 0.21 mm) and the lowest on the level of all teeth surfaces (average lack of deviations, 0.64 mm). Acrylic samples subjected to mechanical testing, as expected, showed higher mechanical properties as compared to the specimens made of dental stone. In the present study we demonstrated that 3D printed acrylic models could be ideal representatives in the case of use as a diagnostic tool and as a part of medical records. The acrylic samples exhibited not only higher mechanical properties, but also showed better accuracy comparing to dental stone.
Laser-Assisted Synthesis and Oxygen Generation of Nickel Nanoparticles
Authors: Wawrzyniak Jakub , Karczewski Jakub , Ryl Jacek , Grochowska Katarzyna , Siuzdak Katarzyna
Published: September 2020

Nowadays, more than ever, environmental awareness is being taken into account when it comes to the design of novel materials. Herein, the pathway to the creation of a colloid of spherical, almost purely metallic nickel nanoparticles (NPs) through pulsed laser ablation in ethanol is presented. A complex description of the colloid is provided through UV-vis spectroscopy and dynamic light scattering analysis, ensuring insight into laser-induced nanoparticle homogenization and size-control of the NPs. The transmission electron spectroscopy revealed spherical nanoparticles with a narrow size distribution, whereas the energy-dispersive X-ray spectroscopy accompanied by the X-ray photoelectron spectroscopy revealed their metallic nature. Furthermore, an example of the application of the colloidal nanoparticles is presented, where a quick, five-min ultrasound modification results in over an order of magnitude higher current densities in the titania-based electrode for the oxygen evolution reaction.
Residually Stressed Fiber Reinforced Solids: A Spectral Approach
Authors: Shariff Mohd Halim Bin Mohd , Merodio Jose
Published: September 2020

We use a spectral approach to model residually stressed elastic solids that can be applied to carbon fiber reinforced solids with a preferred direction; since the spectral formulation is more general than the classical-invariant formulation, it facilitates the search for an adequate constitutive equation for these solids. The constitutive equation is governed by spectral invariants, where each of them has a direct meaning, and are functions of the preferred direction, the residual stress tensor and the right stretch tensor. Invariants that have a transparent interpretation are useful in assisting the construction of a stringent experiment to seek a specific form of strain energy function. A separable nonlinear (finite strain) strain energy function containing single-variable functions is postulated and the associated infinitesimal strain energy function is straightforwardly obtained from its finite strain counterpart. We prove that only 11 invariants are independent. Some illustrative boundary value calculations are given. The proposed strain energy function can be simply transformed to admit the mechanical influence of compressed fibers to be partially or fully excluded.
A New Approach for Designing Fluid Concrete with Low Cement Content: Optimization of Packing Density of Aggregates
Authors: Zhang Ning , Zuo Wenqiang , Xu Wen , Song Shenyou
Published: September 2020

The current study aims at proposing a novel and simple method for designing fluid concrete such as self-compacting concrete (SCC) with a low cementitious binder content to reduce the carbon footprint. Different testing methods regarding the packing density of aggregate mixtures are performed and compared. The W/C was determined according to the target compression strength. Slump flow spread is carried out to determine the most appropriate superplasticizer (SP) dosage and aggregate volume fractions and proportions in concrete mixtures. Furthermore, hardened performance, including compression strength and drying shrinkage of the fluid concrete, are characterized. Finally, a mix design process of fluid concrete with low cement content was proposed based on the preferred fresh and hardened properties of the concrete mixtures.
A Multi-Cell Hybrid Approach to Elevate the Energy Absorption of Micro-Lattice Materials
Authors: Xiao Lijun , Xu Xiao , Song Weidong , Hu Menglei
Published: September 2020

Multi-cell hybrid micro-lattice materials, in which the stretching dominated octet cells were adopted as the strengthen phase while the bending dominated body centered cubic (BCC) lattice was chosen as the soft matrix, were proposed to achieve superior mechanical properties and energy absorption performance. Both stochastic and symmetric distribution of octet cells in the BCC lattice were considered. The cell assembly micromechanics finite element model (FEM) was built and validated by the experimental results. Accordingly, virtual tests were conducted to reveal the stress–strain relationship and deformation patterns of the hybrid lattice specimens. Meanwhile, the influence of reinforcement volume fraction and strut material on the energy absorption ability of the specimens was analyzed. It was concluded that the reinforced octet cells could be adopted to elevate the elastic modulus and collapse strength of the pure BCC micro-lattice material. The multi-cell design could lead to strain hardening in the plateau stress region which resulted in higher plateau stresses and energy absorption capacities. Besides, the symmetric distribution of reinforcements would cause significant stress fluctuations in the plateau region. The obtained results demonstrated that the multi-cell hybrid lattice architectures could be applied to tailor the mechanical behavior and plastic energy absorption performance of micro-lattice materials.
Anti-Neuroinflammatory Agent, Restricticin B, from the Marine-Derived Fungus Penicillium janthinellum and Its Inhibitory Activity on the NO Production in BV-2 Microglia Cells
Authors: Choi Byeoung-Kyu , Jo Song-Hee , Choi Dong-Kug , Trinh Phan Thi Hoai , Lee Hwa-Sun , Cao Cao Van , Van Tran Thi Thanh , Shin Hee Jae
Published: September 2020

A new compound containing a triene, a tetrahydropyran ring and glycine ester functionalities, restricticin B (1), together with four known compounds (2–5) were obtained from the EtOAc extract of the marine-derived fungus Penicillium janthinellum. The planar structure of 1 was determined by detailed analyses of MS, 1D and 2D NMR data. The relative and absolute configurations of 1 were established via the analyses of NOESY spectroscopy data, the comparison of optical rotation values with those of reported restricticin derivatives and electronic circular dichroism (ECD). All the compounds were screened for their anti-neuroinflammatory effects in lipopolysaccharide (LPS)-induced BV-2 microglia cells. Restricticin B (1) and N-acetyl restricticin (2) exhibited anti-neuroinflammatory effects by suppressing the production of pro-inflammatory mediators in activated microglial cells.
Influence of Boron Content on the Solidification Structure, Magnetic Properties and Hot Mechanical Behavior in an Advanced As-Cast TWIP Steel
Authors: Salas-Reyes A. E. , Altamirano-Guerrero G. , Chávez-Alcalá J. F. , Barba-Pingarrón A. , Figueroa I. A. , Bolarín-Miró A. M. , Sánchez-De Jesús F. , Deaquino-Lara R. , Salinas A.
Published: September 2020

This research work reports the effect of boron micro-additions (180 and 470 ppm) on the solidification structure, magnetic properties and hot ductility behavior of an advanced low-carbon highly alloyed twinning-induced plasticity (TWIP) steel. For this purpose, three experimental TWIP steels were fabricated by melting commercial raw materials and casting into metallic molds. Solidification structure was characterized by means of optical and scanning electron microscopy techniques, and a statistical study was carried out to measure dendrite features. A vibrating sample magnetometer was used, at room temperature, to determine magnetic properties, and a X-ray diffraction analysis was performed in order to identify the related phases during magnetic measurements. Finally, the hot ductility in the as-cast condition was evaluated at 700, 900 and 1100 °C, under a constant strain rate of 0.001 s−1. The results indicate that boron micro-additions cause an overall refining solidification structure and austenitic grain size. However, as the boron content increases, segregation of this element promotes formation of ferrite and ε-martensite, leading to ferromagnetic behavior. Nonetheless, with subsequent hot- and cold-rolling, the single austenitic phase is achieved, and this behavior is eliminated. Hot tensile tests revealed that boron micro-addition is beneficial to the hot ductility behavior. The greatest influence was observed for the higher concentration of boron (470 ppm). In comparison with the steel without boron content, the reduction of area (RA) is more than the triple of the hot workability during straining at 900 °C. Thus, present results demonstrate that boron micro-addition has an excellent potential for refining dendritic microstructure and improving the hot-deformation behavior of present low-carbon highly alloyed TWIP steel.