bluuebunny/crossref_metadata_2025 huggingface dataset:
Blood Neutrophil Counts are Associated with Exacerbation Frequency and Mortality in COPD
Authors: Lonergan Mike , Dicker Alison J , Crichton Megan L , Keir Holly R , Dyke Melissa K. Van , Mullerova Hana , Miller Bruce E , Tal-Singer Ruth , Chalmers James
Published: March 2020

Background Identifying patients with COPD at increased risk of poor outcomes is challenging due to disease heterogeneity. Potential biomarkers need to be readily available in real-life clinical practice. Blood eosinophil counts are widely studied but few studies have examined the prognostic value of blood neutrophil counts (BNC). Methods In a large population-based COPD registry in the East of Scotland (TARDIS: Tayside Allergic and Respiratory Disease Information System), BNC were compared to measures of disease severity and mortality over up to 15 years follow-up. Potential mechanisms of disease modification by BNC were explored in a nested microbiome substudy. Results 178120 neutrophil counts were obtained from 7220 people (mean follow up 9 years) during stable disease periods. Median BNC was 5200cells/µL (IQR 4000-7000cells/µL). Mortality rates among those 34% with elevated BNCs (defined as 6000-15000cells/µL) at the study start were 80% higher (14.0/100 person years v 7.8/100py, P<0.001) than those with BNC in the normal range (2000-6000cells/µL). People with elevated BNC were more likely to be classified as GOLD D (46% v 33% P<0.001), have more exacerbations (mean 2.3 v 1.3/year, P<0.001), and were more likely to have severe exacerbations (13% vs. 5%, P<0.001) in the following year. Eosinophil counts were much less predictive of these outcomes. In a sub-cohort (N=276), patients with elevated BNC had increased relative abundance of Proteobacteria and reduced microbiome diversity. Conclusion High BNC may provide a useful indicator of risk of exacerbations and mortality in COPD patients.
Implementing nature-based solutions for creating a resourceful circular city
Authors: Langergraber Guenter , Pucher Bernhard , Simperler Lena , Kisser Johannes , Katsou Evina , Buehler Devi , Mateo Mari Carmen Garcia , Atanasova Nataša
Published: March 2020

Resource depletion, climate change and degradation of ecosystems are challenges faced by cities worldwide and will increase if cities do not adapt. In order to tackle those challenges, it is necessary to transform our cities into sustainable systems using a holistic approach. One element in achieving this transition is the implementation of nature-based solutions (NBS). NBS can provide a range of ecosystem services beneficial for the urban biosphere such as regulation of micro-climates, flood prevention, water treatment, food provision and more. However, most NBS are implemented serving only one single purpose. Adopting the concept of circular economy by combining different types of services and returning resources to the city, would increase the benefits gained for urban areas. The COST Action Circular City aims to establish a network testing the hypothesis that: ‘A circular flow system that implements NBS for managing nutrients and resources within the urban biosphere will lead to a resilient, sustainable and healthy urban environment’. In this paper we introduce the COST Action Circular City by describing its main objectives and aims. The paper also serves as introduction to the review papers of the Action's five Working Groups in this Special Issue.
Opioid Growth Factor and its Derivatives as Potential Non-toxic Multifunctional Anticancer and Analgesic Compounds
Authors: Budka Justyna , Kowalski Szymon , Chylinska Monika , Dzierzbicka Krystyna , Inkielewicz-Stepniak Iwona
Published: March 2020

:

Despite significant research progress on the pathogenesis, molecular biology, diagnosis,
treatment, and prevention of cancer, its morbidity and mortality are still high around the
world. The emerging resistance of cancer cells to anticancer drugs remains still a significant
problem in oncology today. Furthermore, an important challenge is the inability of anticancer
drugs to selectively target tumor cells thus sparing healthy cells.

:

One of the new potential options for efficient and safe therapy can be provided by opioid
growth factor (OGF), chemically termed Met-enkephalin. It is an endogenous pentapeptide
(Tyr-Gly-Gly-Phe-Met) with antitumor, analgesic, and immune-boosting properties. Clinical
trials have demonstrated that OGF therapy alone, as well as in combination with standard
chemotherapies, is a safe, non-toxic anticancer agent that reduces tumor size.

:

In this paper, we review the structure-activity relationship of OGF and its analogues. We
highlight also OGF derivatives with analgesic, immunomodulatory activity and the ability to
penetrate the blood-brain barrier and may be used as safe agents enhancing chemotherapy efficacy
and improving quality of life in cancer patients.

:

The reviewed papers indicate that Met-enkephalin and its analogues are interesting candidates
for the development of novel, non-toxic, and endowed with an analgesic activity anticancer
drugs. More preclinical and clinical studies are needed to explore these opportunities.
Evaluation of Cytotoxic Mechanisms of Clinacanthus Nutans Extracts in Cancer Cells
Authors: Sulaiman Nurul Atiqah , Rajabalaya Rajan , Lee Shirley Huan Fang , Lim Ya Chee , Lim Wei Hon , Roslan Amal Zahirah , David Sheba Rani , Zulkipli Ihsan Nazurah
Published: March 2020

Background:

The preparations of commercially available Clinacanthus nutans (Burm.F)
Lindau (Acanthaceae) (CN) leaf are gaining attention as an alternative cancer treatment, particularly
in South East Asia. Multiple studies have suggested that CN has potential anticancer activities; however,
the mechanism of these activities has remained elusive.

Objective:

This study evaluated the cytotoxic mechanisms of CN extracts in cancer cells.

Methods:

CN extracts were prepared from either fresh or dried leaves, using different solvents. Cytotoxicity
of CN extracts was tested on the A549 (lung cancer), HeLa (cervical cancer), MCF-7
(breast cancer) and MDA-MB-231 (breast cancer) cell lines using the MTT assay. Flow cytometry
was used to assess changes in the cell cycle profile, while Western blotting was used to examine microtubule
stability. Finally, the mode of cell death was investigated using the Annexin V-FITC
Apoptosis Detection Kit.

Results:

Aqueous Fresh (AQF) extract was prepared to simulate the ethnomedicinal use of CN, and
reduce cell viability of MCF-7 cells with IC50 = 1.71 mg/mL. Some CN extracts have the ability to
inhibit the proliferation of four different cancer cell lines after a 24 hour treatment. Annexin V assay
results showed that acetone extracts of CN induced increments in the percentage of apoptotic cell
death. However, flow cytometry results showed that the cancer cell cycle profile was not affected.
Similarly, immunoblotting results also indicate that microtubule dynamics in MCF-7 cells were not
altered. However, the aqueous extract, prepared to simulate the current ethnomedicinal use of CN
leaves in cancer treatment, did not significantly inhibit cancer cell proliferation with an IC50 = 1.71
mg/mL.

Conclusions:

This study was the first to show that microtubules in cancer cells remain dynamic after
treatment with CN extracts, effectively ruling interference of microtubule dynamics as the mode of
cell death. AMD extract showed the highest effects of MCF-7 cell proliferation.
El aborto, una derrota del pensamiento
Authors: Veggetti-Finzi Silvia , Rosenberg Martha
Published: March 2020

El aborto, una derrota del pensamiento
Diez noches de Francisca Lombardo
Authors: Eltit Diamela
Published: March 2020

Diez noches de Francisca Lombardo
A propósito del aborto
Authors: Dolto Francoise
Published: March 2020

A propósito del aborto
Delirio y destino
Authors: Mendiola Salvador
Published: March 2020

Delirio y destino
María Zambrano (1904-1991)
Authors: Mendiola Salvador
Published: March 2020

María Zambrano (1904-1991)
Sobre la representación política femenina
Authors: Cigarini Lia
Published: March 2020

Sobre la representación política femenina
Danzón
Authors: González Rodríguez Sergio
Published: March 2020

Danzón
Comentario al libro La bella (in)diferencia
Authors: Cereijido Fanny Blanck
Published: March 2020

Comentario al libro La bella (in)diferencia
La categoría blanco/no blanco
Authors: Guzmán Jorge
Published: March 2020

La categoría blanco/no blanco
Divagación en torno a los nenúfares
Authors: Martín Gaite Carmen
Published: March 2020

Divagación en torno a los nenúfares
Feminismo y posmodernismo
Authors: Lovibond Sabina , Sefchovich Sara
Published: March 2020

Feminismo y posmodernismo
Repensando el cine de mujeres: teoría estética y feminista
Authors: De Lauretis Teresa , Mayorga Susana
Published: March 2020

Repensando el cine de mujeres: teoría estética y feminista
Tratado de Libre Comercio y las sindicalistas
Authors: Suárez Estela
Published: March 2020

Tratado de Libre Comercio y las sindicalistas
Las amazonas
Authors: Moreno Hortensia
Published: March 2020

Las amazonas
Mujeres del renacimiento
Authors: Hernández Reyes María Adela
Published: March 2020

Mujeres del renacimiento
Sola en el pozo (Opus 135)
Authors: Felipe Liliana
Published: March 2020

Sola en el pozo (Opus 135)
La hija de la pescadora
Authors: K. Le Guin Ursula , Bernal Gloria Elena
Published: March 2020

La hija de la pescadora
El filicidio: tema que horroriza
Authors: De Barbieri Teresita , Malvido Elsa , Torres Antonieta
Published: March 2020

El filicidio: tema que horroriza
Dejar de ser madre
Authors: Coll Rosa
Published: March 2020

Dejar de ser madre
Los hechos de la paternidad
Authors: Parveen Adams , Moreno Hortensia
Published: March 2020

Los hechos de la paternidad
Hacer de madre
Authors: Parveen Adams , Vericat Isabel
Published: March 2020

Hacer de madre
La abnegación: una virtud loca
Authors: Castellanos Rosario
Published: March 2020

La abnegación: una virtud loca
Yo soy de nacimiento cobarde. he temido muchas cosas, pero lo que he temido más es la soledad
Authors: Poniatowska Elena
Published: March 2020

Yo soy de nacimiento cobarde. he temido muchas cosas, pero lo que he temido más es la soledad
Antropología de la mujer: ¿antropología de género, antropología feminista?
Authors: Goldsmith Mary
Published: March 2020

Antropología de la mujer: ¿antropología de género, antropología feminista?
La poesía del español
Authors: Mendiola Salvador
Published: March 2020

La poesía del español
La lección de Mary Beth
Authors: Ehrenreich Barbara
Published: March 2020

La lección de Mary Beth
A su merced (Opus 110)
Authors: Rodríguez Jesusa , Felipe Liliana
Published: March 2020

A su merced (Opus 110)
Fuzzy Inference Methods for Forming Expert Systems for Forecasting Innovative Processes
Authors: Tenenev V. A. , Shatalova O. M.
Published: March 2020

В статье освещены результаты разработки методического обеспечения механизма логического вывода в составе экспертных систем прогнозирования инновационных процессов на промышленных производственных предприятиях.  Актуальность экспертных систем в исследуемой предметной области обусловлена высокой организационной сложностью и существенной неопределенностью инновационных процессов. Логический вывод в составе экспертной системы выполняет функцию воспроизведения логики принятия решений, приемлемой в заданной предметной области. Решение поставленной задачи основано на следующих условиях: логический вывод реализуется по критерию эффективности; эффективность оценивается как мера соответствия между требуемыми и ожидаемыми значениям трех ключевых параметров – целевой эффект, стоимость ресурсов, сроки; показатель эффективности представлен в векторной форме (как трехмерный вектор параметров эффективности); функция соответствия реализуется через нечеткий логический вывод; содержание каждого из параметров эффективности (состав факторов) раскрывается в соответствии с онтологией инновационного процесса; состав факторов эффективности представлен структурной моделью; оценка факторов производится детерминированными и экспертными методами; отношения между факторами реализуются в виде нечетких правил, а также через детерминированные связи; параметры нечеткого логического вывода формируются в соответствии с общей методологией нечетко-множественного моделирования, при этом функция вывода реализуется через взвешенную оценку правил, а весовые коэффициенты определяются экспертными методами;  экспертная оценка весовых коэффициентов проводится в контексте исследуемых стратегий инновационного процесса. Комплексное методическое обеспечение механизма нечеткого логического вывода в экспертной системе прогнозирования инновационных процессов направлено на учет технико-экономических характеристик инновационного процесса, стратегического контекста его реализации, значимых ограничений организационной системы. Разработанные методы нечеткого логического вывода обеспечивают корректный и интуитивно понятный перевод ментальных рассуждений ЛПР (в лингвистической форме) на языковые средства математики; этому способствует в том числе использование метода взвешенной оценки правил при реализации функции вывода, соответствующей ментальным критериям эвристического анализа.
An Evolutionary Approach to Solving the Problem of Vehicle Routing
Authors: Tenenev V. A. , Shaura A. S. , Shaura D. S.
Published: March 2020

Рассматривается задача маршрутизации транспортных средств, являющаяся обобщением задачи коммивояжера. Существует несколько основных моделей маршрутизации, каждая из которых характеризуется наличием дополнительных условий (по грузоподъемности, транспортному парку,  времени доставки, количеству баз и т. д.) и описывает группу реальных  задач транспортной логистики. Эта задача относится к классу NP-трудных, а наличие дополнительных условий еще более усложняет ее решение. В работе описан двухэтапный подход к организации оптимальной маршрутизации: на первом этапе все пункты доставки распределяются на географические районы методами нечеткой кластеризации, а на втором − для каждого района и каждого транспортного средства с помощью генетического алгоритма  рассчитывается оптимальный маршрут. При этом все имеющиеся в задаче ограничения учитываются при вычислении фитнес-функции и определяют допустимость и приспособленность особей популяции. В большинстве практических задач необходимо иметь сбалансированное распределение потребителей по различным маршрутам для равномерной загрузки транспортного парка и снижения общего времени доставки, поэтому  в представленном алгоритме после предварительной оценки продолжительности всех маршрутов предусмотрена дополнительная процедура выравнивания кластеров. Реализованный алгоритм успешно использовался для получения решения задачи маршрутизации в масштабах города и в условиях реальной транспортной сети.
Neural Network Prediction of the Effectiveness of Pharmacotherapy in Hepatitis
Authors: Astafyev A. N. , Voronin S. V. , Vodennikov S. K.
Published: March 2020

Несмотря на проводимую стандартизацию медицинской диагностики, лечение остается и рассматривается как искусство, поскольку для выбора эффективной тактики требуется опыт клинического специалиста. А сама задача определения эффективности предполагает работу с большой неопределенностью, работа, с которой в полном объеме недоступна при применении стандартных алгоритмов в современных вычислительных машинах. Использование методики формирования топологии и обучения нейронных сетей для задачи определения фармакологической эффективности является новой концепцией, исследование в которой позволит привнести некоторую степень объективности в исследовании. В предлагаемой публикации проводится анализ и настройка методики формирования топологии нейронных сетей для оценки эффективности фармакотерапии гепатита, оценку эффективности предлагается производить путем динамической оценки состояния тяжести пациентов. На первоначальном этапе проводится выбор важных клинических факторов, которые главным образом характеризуют состояние течения заболевания, производится выбор шкалы для оценки состояния пациента с заболеванием гепатит. Для предлагаемой оценки состояния рассматривается применение нейронных сетей, выбирается эффективный алгоритм оценки и формирования топологии нейронной сети для рассматриваемой задачи. Проводится сравнение выбранной методики модульного построения топологии нейронной сети с аналогами, которые применяются для медицинских целей. В заключительной части исследования с помощью системы поддержки принятия решения проводится анализ эффективности семи препаратов для фармакотерапии гепатита.
PENGEMBANGAN INSTRUMEN E-TEST SEBAGAI INOVASI PENILAIAN BERBASIS ONLINE DI SEKOLAH DASAR
Authors: Kuncahyono Kuncahyono , Kumalasani Maharani , Aini Dian
Published: March 2020

Perkembangan IPTEK saat ini secara langsung berdampak pada perubahan bentuk pengajaran dalam bidang Pendidikan. Guru dituntut untuk menguasai teknologi untuk menunjang proses pembelajarannya. Salah satu proses dalam pembelajaran yang dapat diimplementasikan dengan kemajuan teknologi adalah proses penilaian. Dalam proses penilaian dibutuhkan pola yang sistematis sehingga mempermudah proses penilaian.SD Tlogomas 2 Malang merupakan salah satu SD yang sudah menerapkan kurikulum 2013. Para guru sudah menerapkan pembelajaran tematik terpadu, dan mencoba mengaplikasikan media dalam kegiatan pembelajaran di kelas. Namun dalam melaksanakan penilaian hanya menggunakan perangkat instrument paper based test. Guru belum menggunakan penilaian secara online.Penelitian ini menggunakan model pengembangan O’ Malley dan Pierce yang disesuaikan dengan kebutuhan penelitian dengan lima tahapan meliputi: 1) menentukan tujuan pengembangan, 2) menyusun spesifikasi produk, 3) melakukan pengembangan, 4) uji coba, dan 5) revisi. Penelitian ini bertujuan untuk ini memfasilitasi siswa dalam meningkatkan kemampuan dan meningkatkan motivasi belajar siswa. Selain itu juga sebagai sarana untuk mempermudah guru dalam melakukan penilaian secara sistematis.
PENINGKATAN KETERAMPILAN MENULIS PUISI MENGGUNAKAN TEKNIK MIND MAPPING DI KELAS V SD.
Authors: Ratih Maistika , Taufina Taufina
Published: March 2020

Abstrak: Based on preliminary studies conducted in class V SDN 01 Koto Merapak. In indoensian language subjects, especially in writing poetry there are still many difficulties and obstacles faced by students. Students find it difficult to express their ideas in the form of poetry. This happens because the learning media used by educators is still less attractive to student, educators have not utilized the media as an object in writing poetry. This causes the skills of student in poetry to be low. The purpose of this study was to describe the improvement of poetry writing skills using the Mind Mapping technique in class V SDN 01 Koto Merapak.  This study uses a qualitative  and quantitative approach, with a type of classroom action research. This research was conducted in two cycles, with the stages of planning, implementing , observing and reflecting. This research was carried out inthe second semester of the 2018/2019 school year at SDN 01 Koto Merapak. With the subject of this research is the fifth grade students of SDN 01 Koto Merapak with a total of 32 student. While the research instruments are observation sheets, field note sheet test sheet, and documentation. The results showed that poetry writing skills using Mind Mapping techniques can improve poetry writing skills of students in SDN 01 SDN Koto Merapak. This can be seen from the result of the poetry  writing ability in the first ccle of meeting I getting an average of 69.78% with  the criterion (C), increasing in the first cycle of the first meeting obtaining an average of  74.12 % with the criteria of good (B), and increasing in the second cycle getting average –average 88.74% with very good criteria (SB). So based on the research above, that intellectual  ability in writing poetry can increase using the Mind Mapping technique.
Growth Parameters of the Silverstripe Blaasop, Lagocephalus sceleratus (Gmelin, 1789) from the Mediterranean Coast of Turkey
Authors: Zengin Kadriye , Türker Dilek
Published: March 2020

Some biological aspects of silverstripe blaasop from the Turkish coast were studied in terms of morphometry age and growth. Samples were collected between December 2011 and May 2013, comprising of 100 inviduals of Lagocephalus sceleratus (Gmelin,1789) from Antalya Bay. The age of each specimen was estimated based on vertebra analysis, and the age of L. sceleratus ranged between one and five years. Length weight relationships were calculated as W= 0.0102TL3.0118 and Von Bertalanffy growth function Lt=79.48 (1-e-0.18537(t+0.61791)). The results were discussed with data available from different studies.
Comparing the Fatty Acid Level of Sand Smelt (Atherina boyeri) With Rainbow Trout (Oncorhynchus mykiss) as a Cheaper Protein and Fatty Acid Source
Authors: Yavuzer Emre
Published: March 2020

Sensorial and fatty acid levels of sand smelt as an alternative protein and fatty acid source against rainbow trout were investigated. The fish were separated into two groups; which were identified in expensive/known one (rainbow trout) and the cheaper/little-known one (sand smelt). Although rainbow trout and sand smelt groups have the same proximate and sensorial values, sand smelt group preferred more according to price/flavor preference. The fatty acid combinations of groups changed from 27.56% to 38.80% saturated (SFA), 26.30–31.22% monounsaturated (MUFAs) and 34.91–41.22% polyunsaturated acids (PUFAs). Between the groups, the highest rates were myristic acid (3.15–3.73%), palmitic acid (15.99–26.85%), palmitoleic acid (5.82–6.01%), stearic acid (4.44–6.40%), oleic acid (11.81–20.40%), linoleic acid (2.47–16.73%), eicosapentaenoic acid (2.30–8.80%) and docosahexaenoic acid (7.84–15.77%). The values of PUFAs-n3 and DHA were higher in a cheaper group than an expensive one.
Taxonomic Status of Endemic Fish Species in Lake Beyşehir Basin (Turkey)
Authors: Bayçelebi Esra , Kaya Cüneyt , Güçlü Salim Serkan , Küçük Fahrettin , Turan Davut
Published: March 2020

The surveys were conducted between 2005-2019 to assess endemic fish species in the Lake Beyşehir that have special importance in terms of endemism. A total of 12 endemic freshwater fish species (Aphanius cf. iconii, Capoeta mauricii, Chondrostoma beysehirense, Garra kemali, Gobio microlepidotus, Pseudophoxinus anatolicus, Pseudophoxinus battalgilae, Pseudophoxinus hittitorum, Squalius anatolicus, Cobitis battalgilae, C. bilseli and Oxynoemacheilus atili) belonging to 6 families (Aphaniidae, Cyprinidae, Gobionidae, Leuciscidae, Cobitidae and Nemachelidae) were observed.
Challenges and social support provisions in the treatment of HIV infected children in Indonesia
Authors: Yuniar Yuyun , Handayani Rini Sasanti
Published: March 2020

Latar belakang: Pengobatan pada anak yang terinfeksi HIV merupakan beban bagi para orang tua/pengasuh karena berbagai permasalahan menyangkut kesehatan mereka.

Metode: Penelitian untuk mengeksplorasi pengalaman dan dukungan sosial pada pengobatan anak terinfeksi HIV dilakukan di 5 provinsi di Indonesia dengan prevalensi HIV tertinggi. Total sampel anak sebanyak 239 orang dari sejumlah 267 orang yang direncanakan. Data dikumpulkan dengan wawancara semi terstruktur dengan orang tua/pengasuhnya. Analisis dilakukan terhadap 16 anak berusia 2-14 tahun yang telah mendapatkan terapi obat antiretroviral.

Hasil: Dari sejumlah 160 anak, sebanyak 62,5% anak mengkonsumsi  1-2 item obat dan 36,9% mengkonsumsi 3 5 item. Efek samping yang paling sering terjadi adalah kulit kemerahan, mual dan muntah. Kesulitan yang paling sering dihadapi adalah rasa bosan dan anak mempertanyakan minum obat. Orangtua/pengasuh berusaha melanjutkan pengobatan dengan mengingatkan jadwal minum obat, membujuk, memberikan penjelasan bahkan memaksa atau mengancam mereka untuk minum obat. Kesulitan tersebut makin bertambah seiring meningkatnya usia anak. Dukungan yang paling sering berasal dari orang tua dan keluarga besar seperti nenek atau paman, khususnya untuk anak yang sudah yatim.

Kesimpulan: Pemahaman hambatan pengobatan pada anak terinfeksi HIV dapat membantu untuk menyediakan intervensi yang tepat untuk meningkatkan kepatuhan yang akan mendorong kesuksesan terapi mereka.

Kata kunci: anak terinfeksi HIV, antiretroviral, dukungan social, pengobatan, kesulitan

 

Abstract

Background: The treatment of HIV infected children is a burden to their caregiver due to many existing problems related with their health.

Methods: A research to explore the experience and social support on the treatment of HIV infected children was conducted in 5 provinces in Indonesia with highest prevalence of HIV. Total children sample was 239 out of previous 267 planned. Data was collected through semi structured interview with caregivers of the children. Analysis was conducted to 160 children aged 2-14 years old who were on antiretroviral therapy.

Results: Among those 160 children, 62.5% took 1-2 items of medicines and 36.9% took 3-5 items. The most frequent adverse events were skin rash followed by nausea and vomiting. Boredom and questioning were the most frequent difficulties. The caregivers attempted to continue the treatment by reminding the children on schedule to take medicines, wheedling, explaining, forcing or even threatening them. The difficulties appeared more as the children grew older. The most frequent supports mainly came from parents, and extended family such as grandmother or uncle especially for orphaned children.

Conclusion: Understanding obstacles in HIV infected children will help to do proper interventions to improve adherence that will lead to successful therapy.

Keywords: HIV infected children, antiretroviral, social support, treatment, difficulties
Cloning and expression of Human Papilloma virus type 16 L1 capsid protein in bacteria
Authors: Widyaningtyas Silvia Tri , Meilany Sofy , Bela Budiman
Published: March 2020

Latar belakang: Secara alamiah protein kapsid L1 Human Papillomavirus (HPV) tipe 16 dapat mengalami auto assembly untuk membentuk Viral like particle (VLP). Terkait dengan penelitian vaksin HPV, VLP dapat digunakan untuk berbagai keperluan seperti vaksin, pseudovirion atau SpyTag-Spycatcher. Penelitian ini ditujukan untuk mendapatkan plasmid rekombinan yang digunakan untuk produksi protein L1 HPV 16.

Metode: Gen penyandi protein L1 HPV 16 diklona ke dalam vector pQE80L, suatu plasmid yang mengandung sistem ekspresi untuk prokariota. DNA penyandi HPV 16 L1 disisipkan pada situs restriksi BamHI dan Hind III plasmid pQE80L. Plasmid rekombinan yang mengandung gen L1 HPV 16dikonfirmasi menggunakan PCR dan analisis enzim restriksi. Lebih lanjut untuk memastikan bahwa gen rekombinan L1 HPV 16 dapat diekspresikan dalam prokariota, plasmid rekombinan ditransformasikan ke bakteri Escherichia coli BL21 (DE3). Bakteri diinduksi dengan Isopropyl β-D-1-thiogalactopyranoside (IPTG) dengan berbagai konsentrasi dan berbagai waktu inkubasi.

Hasil: protein rekombinan L1, berat 56 kDa, telah berhasil diekspresikan dalam sistem prokariota. Protein rekombinan L1 dapat dimurnikan menggunakan TalonR dalam kondisi denaturasi.

Kesimpulan: gen L1 HPV 16 telah dikloning ke dalam pQE80L dan berhasil diekspresikan dalam sistem prokariota. (Health Science Journal of Indonesia 2019;10(2):82-9)

Kata kunci: L1, HPV 16, cervical cancer

 

Abstract

Background: Naturally Human Papillomavirus (HPV) type 16 L1 capsid protein can auto assemble to form Viral like particles (VLP). Concerning to vaccine development for HPV, VLP can be used for a variety of needs such as a vaccine, pseudovirion or SpyTag-Spycatcher. In this study, to obtain a vector expression that can be used in the production of HPV L1 protein, we cloned gene coding HPV 16 L1 protein into pQE80L a plasmid contains an expression system for prokaryote.

Methods: The DNA coding HPV 16 L1 was inserted at BamHI and Hind III restriction sites of pQE80L plasmid. The recombinant plasmid containing the HPV L1 gene was confirmed using PCR colony and enzyme restriction. Further to ensure the recombinant HPV 16 L1 gene could be expressed in a prokaryote, the recombinant plasmid was transformed into bacteria Escherichia coli BL21 (DE3). The bacteria were induced with IPTG with various concentrations and various incubation time.

Result: L1 recombinant protein, 56 kDa in weight, has successfully been expressed in prokaryote system. L1 recombinant protein can be purified using TalonR under denaturing conditions.

Conclusion: L1 HPV 16 gene has been cloned into pQE80L and successfully expressed in prokaryote system. (Health Science Journal of Indonesia 2019;10(2):82-9)

Keywords: L1, HPV 16, cervical cancer
Selected Aspects of Age Management as a Challenge for Polish Entrepreneurs
Authors: Sipa Monika , SkibiÅ„ski Andrzej
Published: March 2020

The process of demographic aging is not only a long-term threat to the maintenance of social security systems, but also a major challenge for the companies in terms of the HR function implementation. It is necessary that employers take a more responsible attitude to the problems of an aging population in order to provide the proper functioning not only of these organisations, but also of the whole society and economy. This paper presents some aspects of age management in the context of the perception of this problem by Polish employers. The main objective of this elaboration was to determine whether and how Polish organisations view the problem of aging of labour force.
Entrepreneurship Development and Market Orientation Model as A Strategy in Food Security in Wamena, Jayawijaya
Authors: Ratang Westim , Syauta Jack H , Tebay Dan Vince
Published: March 2020

Entrepreneurship Development and Market Orientation Model as the strategy in Food Security in Wamena, Jayawijaya.This research is aimed to discover: 1) the mindset of farmer groups (wen mina hipere) about entrepreneurship, 2) the ability of entrepreneurship and market orientation of the farmer groups, 3) The factors that affect the entrepreneurship and the orientation toward the managing ability of garden, fish and sweet potatoes. Population is a group of farmers (wen mina hipere) who work as farmers of sweet potatoes and freshwater fish culturing (carp, catfish and tilapia) that exist in the region of Silokarno Doga District, Wogi village, Wamena. The Samples are 20 farmers as determined by Proportionate cluster random sampling method. Sampling was conducted on a group of farmers (nina hipere) around Wogi village, Wamena. The type of data that will be used are: 1) primary data obtained through the interviews using questionnaire, unstructured interviews, and FGD (Focus Group Dicusion) to 20 farmers (wen mina hipere) of garden, fish and sweet potatoes. 2) secondary data will be obtained from the department of agriculture, the Wamena statistics office. The analysis used is Quantitative analysis with descriptive approach and factors analysis that influence the farmers mindset that consists of entrepreneurship and market orientation variable. The result shows that the variables (1) viewing and taking opportunities, (2) systematic planning, (3) the strategy to influence, (4) confidence, (5) persuasive ability, (6) coordination with related agencies, it does not have a significant effect on the mindset of farmers on entrepreneurship collectively. Variables that have a significant effect on the mindset of farmers on entrepreneurship are: (1) competitor orientation, (2) consumers orientation, (3) specificity, (4) orientation on efficiency, (4) work commitment, (5) Focus on high performance levels, (5) seeking information, (6) persistence and (7) initiative, they significantly and positively affect on the mindset of farmers on entrepreneurship. Dominant variables that make up the success of farmers in Wamena are persistence and work commitment. Entrepreneurship development and market orientation model that can be applied is by providing seedlings and land preparation, land cultivation mentoring, and consumer-oriented in harvest and improving coordination with related agencies for food security.
Effectiveness of Modern Teaching Methods; Evidence from Digital Learning Model of Modern Teaching Methods
Authors: Gul Ejaz
Published: March 2020

This paper elucidates the efficacy of three selected modern and innovative methods of learning by taking a group of 80 students of economics class at university level. Their opinion regarding three selected modern teaching methods was obtained through a questionnaire and statistical analysis of their opinion was carried out which indicated strong tendency towards mutual practice method as 40 out of 80 students (50%) declared it as very effective method of learning in the practice stage. On the other hand, 30 students (37.5%) opined that controlled practice method is moderately effective and 28 (35%) students opined that team practice method is slightly effective. After this analysis, students were put to practically learn use of econometric software â€˜E Viewsâ€™ through the same three selected methods. The digital model for their learning process was created using Computer Assisted Qualitative Data Assisted Software (CAQDAS). The statistical analysis of studentsâ€™ opinion and digital analysis of practical learning process indicated that mutual practice is the most effective method of practice. It is because students learn better and fast when they are allowed to use their initiative and judgment. At the end, guidelines for effective teaching have been suggested.
Effects of the fixed orthodontic therapy on biochemical and microbiological parameters of saliva
Authors: Ivanovic Tanja , Stojanovic Ljiljana , Ivanovic Dragan , Nikolic Predrag , Milosavljevic Zeljko , Milinkovic Marina
Published: March 2020

Introduction/Objective. Malocclusions are one of the most frequent disorders in dentistry, and pose a risk for the onset of caries and periodontal diseases. Fixed orthodontic treatment solves the problem of malocclusions; however, it requires simultaneous cooperation of the patients, parents, and dentists involved. The objective of this study is to examine the effects of fixed orthodontic therapy on the Streptococcus mutans and Lactobacillus spp. bacteria in saliva, the pH value, and buffering capacity of saliva. Methods. The research was carried out at the Faculty of Medicine in Foca, Department of Dentistry. The study included 100 respondents, aged 13 to 17 years. The respondents were divided into two groups: the study group (respondents wearing fixed braces) and the control group (respondents not subjected to fixed braces therapy). Saliva samples were taken from the respondents four, 12, and 18 weeks after the start of the orthodontic therapy. The study used the bacteria caries risk test (CRT) and CRT buffer (Ivoclar Vivadent). Results. The study showed an increased number of bacteria in saliva of the respondents during all three follow-up periods (?2 test, p = 0.001). The largest numbers of the Streptococcus mutans and Lactobacillus spp. bacteria were found in week 12 of the therapy. Saliva pH value and buffering capacity of saliva increased statistically significantly in week 12 of the therapy (?2 test, p = 0.001). Conclusion. Oral conditions in patients changed during the fixed orthodontic therapy: the number of bacteria increased, the pH value and buffering capacity of saliva changed. It was necessary to use preventive measures in order to avoid complications during the fixed orthodontic appliances therapy.
Writing kinematics and graphic rules in children with ADHD
Authors: Ivancevic Nikola , Miler-Jerkovic Vera , Stevanovic Dejan , Jancic Jasna , Popovic Mirjana
Published: March 2020

Introduction/Objective. The aim of this study was to coIntroduction/Objective. The aim of this study was to compare kinematic features and graphic rules of writing between children with attention deficit/hyperactivity disorder (ADHD) (with and without medical treatment) and typically developed children (TDC). Methods. In total, 55 children (26 with ADHD/ten subjects were on methylphenidate treatment and 29 TDC) completed a writing task on a digitizing board (in three repetitions; using non-inking stylus) which included a semicircle tracing, triangle, and letter copying. Kinematic features of movements in all tasks and graphic rules during a semicircle tracing were analyzed. Graphic rules were observed as expected movements (selecting the starting point and direction of tracing). Results. The values of kinematic parameter jerk were significantly larger in TDC group compared to all ADHD subjects (regardless of treatment) and increased constantly with semicircle task progression and repetition in both groups. Children with ADHD without methylphenidate treatment used overall slower movements compared to TDC. The tracing of children with ADHD taking methylphenidate was more automated (with less change in movement velocity and acceleration) compared to TDC. In ADHD group only, those with treatment traced faster and more automated compared to those without treatment. The majority of subjects used expected movements in semicircle tracing and this percentage increased with the task repetition (without difference between ADHD and TDC). Conclusion. Both children with ADHD and TDC used similar approach in the tracing task and were compliant with graphic rules. Methylphenidate treatment may positively influence writing kinematics in children with ADHD. Task repetition also influences writing.
Hubungan Minat Belajar Dengan Hasil Belajar Pada Pembelajaran Sosiologi Pada Kelas XII IPS di SMAN 5 Padang
Authors: Rosalina Lusiana , Junaidi Junaidi
Published: March 2020

Penelitian ini memfokuskan pada hubungan minat belajar siswa dengan hasil belajar siswa. Tujuan penelitian ini untuk melihat korelasi minat belajar dengan hasil belajar siswa pada pembelajaran sosiologi kelas XII IPS di SMAN 5 Padang. Faktor yang diteliti meruakan faktor internal yaitu minat, adapun indikator minat yang diteliti adalah adanya rasa ketertarikan terhadap pelajaran, adanya pemusatan perhatian, adanya keingintahuan yang besar, adanya kebutuhan terhadap pelajaran dan perasaan senang dalam belajar. Penelitian ini adalah penelitian expost facto. Populasi pada penelitian ini sebanyak 93 orang, jumlah sampel sebanyak 75 responden menggunakan rumus Arikunto. Sampel setiap kelas ditentukan dengan proportional random sampling. Data dikumpulkan mengunakan angket. Hasil yang di dapat menunjukkan bahwa adanya korelasi yang signifikan antara variabel minat belajar dengan hasil belajar sosiologi yang diperoleh peserta didik kelas XII IPS, hal ini dibuktikan dengan adanya koefisien korelasi (rxy )1,99 > rtabel 0,227 dan juga dari indikator minat belajar mendapatkan hasil bahwa semua indikator mempunyai hubungan atau berkorelasi dengan hasil belajar.
Planejamento e controle de produção de uma pequena indústria de equipamentos para o setor leiteiro
Authors: Daros Vanessa Sinelli , Reis Zaida Cristiane dos , Matte Juliana , Chais Cassiane , Ganzer Paula Patricia , Olea Pelayo Munhoz
Published: March 2020

O planejamento e controle da produção é necessário para que a empresa obtenha maior eficiência, minimize seus desperdícios e atrasos de entrega de produtos. Portanto, este estudo teve como objetivo avaliar o processo produtivo de uma pequena indústria, tendo como principal objeto de estudo o seu planejamento e controle de produção, de forma que se possam identificar as variáveis que interferem no seu processo produtivo e sugerir melhorias. Assim, partiu-se de uma pesquisa bibliográfica, mostrando conceitos e visão de autores sobre Sistemas de Produção e Planejamento e Controle de Produção (PCP). Após observada a atual situação do processo produtivo da empresa estudada, foi sugerida a implementação de um setor de PCP e elaborado um guia simplificado para auxiliar na implantação.
Palavras-chave: Planejamento. Controle. Produção.
RESPONSE OF HEAVY METAL CONTENTS IN APRICOTS TO DIFFERENT TRANSPORTATION MODES
Authors: Karlidağ Hüseyin , Turan Metin , Karaat Fırat Ege , Ozlu Ekrem , Arriaga Francisco , Kan Tuncay , Atay Salih
Published: March 2020

In order to evaluate the effects of different transportation hubs on cultivated soil and apricots, macro and micro elements and heavy metal contents of fruit, leaf, kernel and soil samples collected from apricot orchards located at the border of the railroad, the motorway, the airport, and an orchard far from transportation modes were detected by ICP/OES (inductively coupled plasma / optical emission spectrometry). The results indicated the highest Cd, Pb and Ni contents of soil, fruit, and kernel samples under impacts of railroad transportation modes, whereas the highest contents of leaf were found under motorway side. All fruit samples contained higher amounts of Cd and Pb compared to permissible limits of FAO/WHO, and contents differentiated between sampling locations. There were no correlative relations found between transportation modes and macro-micro element contents. As a conclusion, in terms of heavy metal contamination, the orchards located at railway sides have the highest risk and this was followed by motorway side.
Biography work in in long-term residential aged care with tablet support to improve the quality of life and communication – study protocol for app development and evaluation / Biografiearbeit in Senioreneinrichtungen mit Tablet-Unterstützung zur Verbesserung der Lebensqualität und Kommunikation (BaSeTaLK) – Studienprotokoll zur App-Entwicklung und Evaluation
Authors: Corsten Sabine , Lauer Norina
Published: March 2020

Background

Older adults in care facilities face a high risk of experiencing depression. The impact that early interventions like biographical work have on the quality of life for older adults in such facilities is unknown.

Aim

To develop and evaluate a tablet-supported intervention for biographical work in long-term residential aged care to increase the quality of life for older adults.

Design

The study will be conducted in a randomized pretest–posttest control group design with follow-up testing in group and single settings. Participants will be randomized to the experimental intervention (tablet-supported biographic work) or the control intervention (planned tablet-supported game playing), each guided by senior volunteers. A total of 80 residents and 16 volunteers will be recruited. The primary outcome for the residents and volunteers will be quality of life as measured with the World Health Organization Quality of Life Assessment-for older adults. Secondary measures will be self-esteem and life satisfaction. In addition, we will examine residents’ ability to communicate and their functional independence.

Method

The first stage of the project involves developing an app. The app is developed in a user-centered, agile development process. It will use multimedia to prepare life history topics and links them to key questions. Next, a workshop is developed for the volunteers who accompany the use of the app in the institutions. During the second phase, biographic work stimulated by the app will be conducted in groups or individually with residents.

Discussion

This is the first known program tailored to older adults in care facilities and senior volunteers that aims to prevent depression by providing digitally supported biographic work.
Intensitas Penyakit Busuk Pangkal Batang Pada Tanaman Lada (Piper nigrum L) Di Desa Batuah Kecamatan Loa Janan Kabupaten Kutai Kartanegara
Authors: Akhsan Ni'matuljannah , Patangke Albert
Published: March 2020

The Malonan-1 variety is a pepper plant originating from East Kalimantan which has become a national superior pepper. This pepper contains around 2,35% essential oil, oleoserin 11,23% and piperine 3,82% and tolerance to stem rot disease. Preliminary survey results in the village of Batuah, Kutai Kartanegara District, East Kalimantan, it was found that the stem rot disease in Malonan-1 pepper, therefore it is necessary to conduct research to find out how much the intensity of the stem rot disease at this time and identify fungi that cause stem rot rot disease in pepper plants. This research was conducted for 5 months starting from January to May 2019. The research location was in Batuah Village, Loa Janan Subdistrict, Kutai Kartanegara District and in the Plant Disease Pest Laboratory, Faculty of Agriculture, Mulawarman University. Research includes activities in the field and in the laboratory. The activity in the field is a direct survey of eight pepper farmer groups and calculating the intensity of the disease. Also carried out samples of symptomatic stem rot disease to be brought to the laboratory. Activities in the laboratory are the preparation of tools and materials to isolate samples of pepper plants. The isolated fungal colonies were purified and then idenfied and continued with Koch's postulate. The average intensity of stem rot disease at the current Lada plant in Batuah village is 14,58% (mild), the highest intensity in the Young Lada farmer group is 26,52% and the lowest intensity in the Beringin Jaya Farmer group is 4.3%. Based on the results of isolation, the root disease of the pepper plant (Piper nigrum L) in Batuah Village, Loa Janan District, Kutai Kartanegara Regency is Phytophthora capsici L.
The Reimagining Kinship, Gender, and Sexuality in Indigenous Communities Colloquium: An Overview and Reflective Essay
Authors: Stirrett Natasha Ermineskin
Published: March 2020

The following provides highlights from the Reimagining Kinship, Gender, and Sexuality in Indigenous Communities Colloquium, a one-day event held in Kingston, Ontario at Queen’s University in January 2019. It was hosted by the Journal of Critical Race Inquiry (JCRI) and the Department of Gender Studies, Sexual and Gender Diversity Certificate Program. The overarching aim of the day was to provide a space for meaningful dialogue on gender, sexuality, cultural revitalization and relations within Indigenous communities. This special section of this Journal of Critical Race Inquiry (JCRI) issue highlights some of the Indigenous intellectual work emerging from the Colloquium. The written pieces presented here cover some of the themes related to love, two-spirit identities, governance, kinship, consent, storytelling, and belonging. It is our hope the Reimagining Kinship, Gender and Sexuality in Indigenous Communities Colloquium and this special report from JCRI will spark further conversations and ideas that will contribute to the cultural resurgence of our Indigenous communities and knowledges across Turtle Island and among all our relations—human and more-than-human.
Digital Image Denoising Techniques in Wavelet Domain with another Filter: A review
Authors: Ferzo Barwar M. , Mustafa Firas M.
Published: March 2020

Image denoising is a challenging issue found in diverse image processing and computer vision problems. There are various existing methods investigated to denoising image. The essential characteristic of a successful model that denoising image is that it should eliminate noise as far as possible and edges preserving and necessary image information by improving visual quality. This paper presents a review of some significant work in the field of image denoising based on that the denoising methods can be roughly classified as spatial domain methods, transform domain methods, or can mix both to get the advantages of them. This work tried to focus on this mixing between using wavelet transform and the filters in spatial domain to show spatial domain. There have been numerous published algorithms, and each approach has its assumptions, advantages, and limitations depending on the various merits and noise. An analyzing study has been performed comparative in their methods to achieve the denoising algorithms, filtering approach and wavelet-based approach. Standard measurement parameters have been used to compute results in some studies to evaluate techniques while other methods applied new measurement parameters to evaluate the denoising techniques.