bluuebunny/crossref_metadata_2025 huggingface dataset:
Whitebark Pine in Crater Lake and Lassen Volcanic National Parks: Assessment of Stand Structure and Condition in a Management and Conservation Perspective
Authors: Jackson Jenell I. , Smith Sean B. , Nesmith Jonathan C.B. , Starcevich Leigh Ann , Hooke Jennifer S. , Buckley Steve , Jules Erik S.
Published: September 2019

Whitebark pine (Pinus albicaulis. Engelm.) is vulnerable to a number of threats including an introduced pathogen (Cronartium ribicola J.C. Fisch.), epidemic levels of native mountain pine beetle (Dendroctonus ponderosae Hopkins), fire suppression, and climate change. To describe the structure of whitebark pine populations in two national parks in the southern Cascades (Crater Lake, Oregon, USA (CRLA) and Lassen Volcanic, California, USA (LAVO) National Parks), we surveyed trees in 30 × 50 × 50 m plots in both parks. We used these plots to describe the extent of white pine blister rust (the disease caused by Cronartium ribicola), mountain pine beetle occurrence, and to elucidate factors influencing the presence of pests and pathogens, cone production, and canopy kill. In each plot, we recorded data related to tree health, including symptoms of blister rust and mountain pine beetle, and reproductive vigor (cone production). In both parks, encroachment from other species, particularly mountain hemlock (Tsuga mertensiana (Bong.) Carrière), was negatively associated with cone production. In CRLA, water stress was a good predictor of blister rust infection and cone production. For CRLA and LAVO, the presence of mountain pine beetle and blister rust was associated with higher canopy kill for whitebark pine. Lastly, we found evidence for a pest-pathogen interaction, mountain pine beetle attack was greater for trees that showed symptoms of blister rust infection in CRLA. Our results indicate that whitebark pine populations in the southern Cascade Range are experiencing moderate levels of blister rust infection compared with other sites across the species range, and that competition from shade-tolerant species may result in an additional threat to whitebark pine in both parks. We present our findings in the context of park management and situate them in range-wide and regional conservation strategies aimed at the protection and restoration of a declining species.
Xylose-Enriched Ethanol Fermentation Stillage from Sweet Sorghum for Xylitol and Astaxanthin Production
Authors: Stoklosa Ryan J. , Nghiem Nhuan P. , Latona Renee J.
Published: September 2019

Developing integrated biorefineries requires the generation of high-value co-products produced alongside cellulosic ethanol. Most industrial yeast strains produce ethanol at high titers, but the small profit margins for generating ethanol require that additional high-value chemicals be generated to improve revenue. The aim of this research was to boost xylose utilization and conversion to high-value co-products that can be generated in an integrated biorefinery. Pretreated sweet sorghum bagasse (SSB) was hydrolyzed in sweet sorghum juice (SSJ) followed by ethanol fermentation. Ethanol was removed from the fermentation broth by evaporation to generate a stillage media enriched in xylose. Candida mogii NRRL Y-17032 could easily grow in non-detoxified stillage media, but a high xylitol yield of 0.55 g xylitol/g xylose consumed was achieved after recovered cells were resuspended in synthetic media containing supplemented xylose. Phaffia rhodozyma ATCC 74219 could be cultivated in non-detoxified stillage media, but astaxanthin generation was increased 4-fold (from 17.5 to 71.7 mg/L) in detoxified media. Future processing strategies to boost product output should focus on a two-step process where the stillage media is used as the growth stage, and a synthetic media for the production stage utilizing xylose generated from SSB through selective hemicellulase enzymes.
Dynamic Group Recommendation Based on the Attention Mechanism
Authors: Xu Haiyan , Ding Yanhui , Sun Jing , Zhao Kun , Chen Yuanjian
Published: September 2019

Group recommendation has attracted significant research efforts for its importance in benefiting group members. The purpose of group recommendation is to provide recommendations to group users, such as recommending a movie to several friends. Group recommendation requires that the recommendation should be as satisfactory as possible to each member of the group. Due to the lack of weighting of users in different items, group decision-making cannot be made dynamically. Therefore, in this paper, a dynamic recommendation method based on the attention mechanism is proposed. Firstly, an improved density peak clustering ( DPC ) algorithm is used to discover the potential group; and then the attention mechanism is adopted to learn the influence weight of each user. The normalized discounted cumulative gain (NDCG) and hit ratio (HR) are adopted to evaluate the validity of the recommendation results. Experimental results on the CAMRa2011 dataset show that our method is effective.
Detection of Differentially Methylated Regions Using Bayes Factor for Ordinal Group Responses
Authors: Dunbar Fengjiao , Xu Hongyan , Ryu Duchwan , Ghosh Santu , Shi Huidong , George Varghese
Published: September 2019

Researchers in genomics are increasingly interested in epigenetic factors such as DNA methylation, because they play an important role in regulating gene expression without changes in the DNA sequence. There have been significant advances in developing statistical methods to detect differentially methylated regions (DMRs) associated with binary disease status. Most of these methods are being developed for detecting differential methylation rates between cases and controls. We consider multiple severity levels of disease, and develop a Bayesian statistical method to detect the region with increasing (or decreasing) methylation rates as the disease severity increases. Patients are classified into more than two groups, based on the disease severity (e.g., stages of cancer), and DMRs are detected by using moving windows along the genome. Within each window, the Bayes factor is calculated to test the hypothesis of monotonic increase in methylation rates corresponding to severity of the disease versus no difference. A mixed-effect model is used to incorporate the correlation of methylation rates of nearby CpG sites in the region. Results from extensive simulation indicate that our proposed method is statistically valid and reasonably powerful. We demonstrate our approach on a bisulfite sequencing dataset from a chronic lymphocytic leukemia (CLL) study.
Identification of Two Independent COL5A1 Variants in Dogs with Ehlers–Danlos Syndrome
Authors: Bauer Anina , Bateman John F. , Lamandé Shireen R. , Hanssen Eric , Kirejczyk Shannon G.M. , Yee Mark , Ramiche Ali , Jagannathan Vidyha , Welle Monika , Leeb Tosso , Bateman Fiona L.
Published: September 2019

The Ehlers–Danlos syndromes (EDS) are a heterogeneous group of heritable disorders affecting connective tissues. The mutations causing the various forms of EDS in humans are well characterized, but the genetic mutations causing EDS-like clinical pathology in dogs are not known, thus hampering accurate clinical diagnosis. Clinical analysis of two independent cases of skin hyperextensibility and fragility, one with pronounced joint hypermobility was suggestive of EDS. Whole-genome sequencing revealed de novo mutations of COL5A1 in both cases, confirming the diagnosis of the classical form of EDS. The heterozygous COL5A1 p.Gly1013ValfsTer260 mutation characterized in case 1 introduced a premature termination codon and would be expected to result in α1(V) mRNA nonsense-mediated mRNA decay and collagen V haploinsufficiency. While mRNA was not available from this dog, ultrastructural analysis of the dermis demonstrated variability in collagen fibril diameter and the presence of collagen aggregates, termed ‘collagen cauliflowers’, consistent with COL5A1 mutations underlying classical EDS. In the second case, DNA sequencing demonstrated a p.Gly1571Arg missense variant in the COL5A1 gene. While samples were not available for further analysis, such a glycine substitution would be expected to destabilize the strict molecular structure of the collagen V triple helix and thus affect protein stability and/or integration of the mutant collagen into the collagen V/collagen I heterotypic dermal fibrils. This is the first report of genetic variants in the COL5A1 gene causing the clinical presentation of EDS in dogs. These data provided further evidence of the important role of collagen V in dermal collagen fibrillogenesis. Importantly, from the clinical perspective, we showed the utility of DNA sequencing, combined with the established clinical criteria, in the accurate diagnosis of EDS in dogs.
Aberrant Splicing Events Associated to CDH23 Noncanonical Splice Site Mutations in a Proband with Atypical Usher Syndrome 1
Authors: Valero Rebeca , de Castro-Miró Marta , Jiménez-Ochoa Sofía , Rodríguez-Ezcurra Juan José , Marfany Gemma , Gonzàlez-Duarte Roser
Published: September 2019

Aims: The aim of this study was the genetic diagnosis by next generation sequencing (NGS) of a patient diagnosed with Usher syndrome type 2 and the functional evaluation of the identified genetic variants to establish a phenotype–genotype correlation. Methods: Whole exome sequencing (WES) analysis identified two heterozygous intronic variants in CDH23, a gene responsible of Usher syndrome type 1. Evaluation of the putative splicing effects was performed in vivo, in whole blood samples, and in vitro, by transfection of midigene constructs in HEK293T cells. Results: Two intronic variants were identified in intron 45 of CDH23—one novel, c.6050-15G>A, and the other, c.6050-9G>A, already reported as a noncanonical splice site (NCSS) mutation—with partial functional characterization. In vivo and in vitro analyses showed aberrant transcripts by the addition of 13 and 7 nucleotides to exon 46, respectively. Transcript degradation by nonsense mediated decay (NMD) in blood cells could only be prevented by cycloheximide treatment. Midigene constructs showed that the two variants contributed to exon skipping and generated aberrantly spliced transcripts. Conclusions: A combination of in vivo and in vitro assays provided a comprehensive view of the physiological effects of NCSS variants, which in this case led to a clinical reassignment of the proband as affected with atypical USH1 syndrome.
Transcriptomic Signatures of Experimental Alkaloid Consumption in a Poison Frog
Authors: Sanchez Eugenia , Rodríguez Ariel , Grau Jose H. , Lötters Stefan , Künzel Sven , Saporito Ralph A. , Ringler Eva , Schulz Stefan , Wollenberg Valero Katharina C. , Vences Miguel
Published: September 2019

In the anuran family Dendrobatidae, aposematic species obtain their toxic or unpalatable alkaloids from dietary sources, a process known as sequestering. To understand how toxicity evolved in this family, it is paramount to elucidate the pathways of alkaloid processing (absorption, metabolism, and sequestering). Here, we used an exploratory skin gene expression experiment in which captive-bred dendrobatids were fed alkaloids. Most of these experiments were performed with Dendrobates tinctorius, but some trials were performed with D. auratus, D. leucomelas and Allobates femoralis to explore whether other dendrobatids would show similar patterns of gene expression. We found a consistent pattern of up-regulation of genes related to muscle and mitochondrial processes, probably due to the lack of mutations related to alkaloid resistance in these species. Considering conserved pathways of drug metabolism in vertebrates, we hypothesize alkaloid degradation is a physiological mechanism of resistance, which was evidenced by a strong upregulation of the immune system in D. tinctorius, and of complement C2 across the four species sampled. Probably related to this strong immune response, we found several skin keratins downregulated, which might be linked to a reduction of the cornified layer of the epidermis. Although not conclusive, our results offer candidate genes and testable hypotheses to elucidate alkaloid processing in poison frogs.
Developmental and Molecular Changes Underlying the Vernalization-Induced Transition to Flowering in Aquilegia coerulea (James)
Authors: Sharma Bharti , Batz Timothy A. , Kaundal Rakesh , Kramer Elena M. , Sanders Uriah R. , Mellano Valerie J. , Duhan Naveen , Larson Rousselene B.
Published: September 2019

Reproductive success in plants is dependent on many factors but the precise timing of flowering is certainly among the most crucial. Perennial plants often have a vernalization or over-wintering requirement in order to successfully flower in the spring. The shoot apical meristem undergoes drastic developmental and molecular changes as it transitions into inflorescence meristem (IM) identity, which then gives rise to floral meristems (FMs). In this study, we have examined the developmental and gene expression changes underlying the transition from the vegetative to reproductive phases in the basal eudicot Aquilegia coerulea, which has evolved a vernalization response independently relative to other established model systems. Results from both our histology and scanning electron studies demonstrate that developmental changes in the meristem occur gradually during the third and fourth weeks of vernalization. Based on RNAseq data and cluster analysis, several known flowering time loci, including AqFT and AqFL1, exhibit dramatic changes in expression during the fourth week. Further consideration of candidate gene homologs as well as unexpected loci of interest creates a framework in which we can begin to explore the genetic basis of the flowering time transition in Aquilegia.
Tsunami Vulnerability Criteria for Fishery Port Facilities in Japan
Authors: Imai Kentaro , Inazumi Takatomi , Emoto Kuniaki , Horie Takehito , Suzuki Akira , Kudo Keita , Ogawa Masami , Noji Masataka , Mizuto Keisuke , Sasaki Takayuki
Published: September 2019

Business continuity plans (BCPs) can be effective and proactive countermeasures to respond flexibly to crises caused by unexpected natural disasters such as large tsunamis. BCP guidelines for fisheries are being issued by the Fisheries Agency (Japan) and the utilization of such BCPs has been spreading in Japan. Despite the need to promote the social implementation of BCPs in the fisheries industry, there is a lack of quantitative criteria for the tsunami damage threshold of various fishing ports and facilities. In this research, we surveyed the damage to the fishing facilities from the tsunami generated by the 2011 Tohoku-oki earthquake. Data were collected on the tsunami damage for various fishery facilities and used to establish criteria for tsunami damage thresholds. In addition, tsunami damage inferred from 1506 scenarios of tsunami computations was predicted probabilistically for a model region as the Nachi Katsu’ura Fishery Port in Wakayama Prefecture. The method we developed in this research makes it possible to assess the probabilistic tsunami damage by combining a tsunami hazard assessment method with criteria for damage occurrence for fishery port facilities and equipment.
Mapping Geospatial Processes Affecting the Environmental Fate of Agricultural Pesticides in Africa
Authors: Hendriks Chantal M. J. , Gibson Harry S. , Trett Anna , Python André , Weiss Daniel J. , Vrieling Anton , Coleman Michael , Gething Peter W. , Hancock Penny A. , Moyes Catherine L.
Published: September 2019

The application of agricultural pesticides in Africa can have negative effects on human health and the environment. The aim of this study was to identify African environments that are vulnerable to the accumulation of pesticides by mapping geospatial processes affecting pesticide fate. The study modelled processes associated with the environmental fate of agricultural pesticides using publicly available geospatial datasets. Key geospatial processes affecting the environmental fate of agricultural pesticides were selected after a review of pesticide fate models and maps for leaching, surface runoff, sedimentation, soil storage and filtering capacity, and volatilization were created. The potential and limitations of these maps are discussed. We then compiled a database of studies that measured pesticide residues in Africa. The database contains 10,076 observations, but only a limited number of observations remained when a standard dataset for one compound was extracted for validation. Despite the need for more in-situ data on pesticide residues and application, this study provides a first spatial overview of key processes affecting pesticide fate that can be used to identify areas potentially vulnerable to pesticide accumulation.
Salt Reduction Intervention in Families Investigating Metabolic, Behavioral and Health Effects of Targeted Intake Reductions: Study Protocol for a Four Months Three-Armed, Randomized, Controlled “Real-Life” Trial
Authors: Bjoernsbo Kirsten Schroll , Riis Nanna Louise , Andreasen Anne Helms , Petersen Janne , Dahl Lassen Anne , Trolle Ellen , Frederiksen Amalie Kruse Sigersted , Munk Jens Kristian , Toft Ulla
Published: September 2019

Reductions in salt intake have the potential to markedly improve population health at low cost. Real life interventions that explore the feasibility and health effects of a gradual salt reduction lasting at least four weeks are required. The randomized controlled SalT Reduction InterVEntion (STRIVE) trial was developed to investigate the metabolic, behavioral and health effects of four months of consuming gradually salt reduced bread alone or in combination with dietary counselling. This paper describes the rationale and methods of STRIVE. Aiming at 120 healthy families, participants were recruited in February 2018 from the Danish Capital Region and randomly allocated into: (A) Salt reduced bread; (B) Salt reduced bread and dietary counseling; (C) Standard bread. Participants were examined before the intervention and at four months follow-up. Primary outcome is change in salt intake measured by 24 h urine. Secondary outcomes are change in urine measures of potassium and sodium/ potassium ratio, blood pressure, plasma lipids, the renin-angiotensin system, the sympathetic nervous response, dietary intake as well as salt taste sensitivity and preferences. The results will qualify mechanisms affected during a gradual reduction in salt intake in compliance with the current public health recommendations.
Relationships Between Smartphone Dependency and Aggression Among Middle School Students: Mediating and Moderating Effects of Ego-Resilience, Parenting Behaviour, and Peer Attachment
Authors: Um Youn-Joo , Choi Yun-Jung , Yoo So Yeon
Published: September 2019

This study examined the moderating and mediating effects of ego-resilience, parenting behaviour, and peer attachment on the relationship between smartphone dependency and aggression. Participants included 1863 middle school youths who used smartphones and had responded to the 7th Korean Children and Youth Panel Survey (KCYPS), which was conducted by Korea’s National Youth Policy Institute. Data were analysed using descriptive statistics, correlation, and hierarchical regression analyses. First, ego-resilience showed a partial mediating effect on the relationship between smartphone dependency and aggression (significant moderating effects were revealed). Second, parenting behaviour demonstrated a partial mediating effect on the relationship between smartphone dependency and aggression (no moderating effects observed). Third, peer attachment had a partial mediating effect on the relationship between smartphone dependency and aggression (no moderating effects were observed). These findings suggest that mental health and student growth can be improved as children develop by implementing various nursing and health care programs designed to improve ego-resilience, parenting behaviour, and peer attachment.
Influence of Occupational Stress and Coping Style on Periodontitis among Japanese Workers: A Cross-Sectional Study
Authors: Islam Md Monirul , Ekuni Daisuke , Yoneda Toshiki , Yokoi Aya , Morita Manabu
Published: September 2019

The aim of this cross-sectional study was to evaluate the association between the influence of occupational stress and coping style on periodontitis among Japanese workers. The study sample included 738 workers (age range: 19–65 years) at a manufacturing company in Kagawa Prefecture, Japan. To analyze occupational stress and coping style, all participants answered a self-report questionnaire composed of items on their work environment and oral health behavior. Oral examinations were performed by calibrated dentists. Among all workers, 492 (66.7%) workers were diagnosed with periodontitis, and 50 (6.8%) were diagnosed with a high stress-low coping condition. Significant differences (p < 0.05) were observed between the periodontitis and non-periodontitis groups in terms of age, gender, body mass index, smoking status, daily alcohol drinking, monthly overtime work, worker type, and stress-coping style. Logistic regression analysis showed that a high stress–low coping condition was associated with an increased risk of periodontitis (odds ratio: 2.79, 95% confidence interval: 1.05–7.43, p = 0.039). These findings suggest that a high stress-low coping condition is associated with periodontitis among the 19–65 years of age group of Japanese workers.
Selecting a Bedside Cognitive Vital Sign to Monitor Cognition in Hospital: Feasibility, Reliability, and Responsiveness of Logical Memory
Authors: Nicholas Padraic , O’Caoimh Rónán , Gao Yang , Habib Afsana , Mross Thomas Karol , Clarnette Roger , Molloy D. William
Published: September 2019

Although there is a high prevalence of delirium and cognitive impairment among hospitalised older adults, short, reliable cognitive measures are rarely used to monitor cognition and potentially alert healthcare professionals to early changes that might signal delirium. We evaluated the reliability, responsiveness, and feasibility of logical memory (LM), immediate verbal recall of a short story, compared to brief tests of attention as a bedside “cognitive vital sign” (CVS). Trained nursing staff performed twice-daily cognitive assessments on 84 clinically stable inpatients in two geriatric units over 3–5 consecutive days using LM and short tests of attention and orientation including months of the year backwards. Scores were compared to those of an expert rater. Inter-rater reliability was excellent with correlation coefficients for LM increasing from r = 0.87 on day 1 to r = 0.97 by day 4 (p < 0.0001). A diurnal fluctuation of two points from a total of 30 was deemed acceptable in clinically stable patients. LM scores were statistically similar (p = 0.98) with repeated testing (suggesting no learning effect). All nurses reported that LM was feasible to score routinely. LM is a reliable measure of cognition showing diurnal variation but minimal learning effects. Further study is required to define the properties of an ideal CVS test, though LM may satisfy these.
New Insights on the Role of Manganese in Alzheimer’s Disease and Parkinson’s Disease
Authors: Martins Airton Cunha , Morcillo Patricia , Ijomone Omamuyovwi Meashack , Venkataramani Vivek , Harrison Fiona Edith , Lee Eunsook , Bowman Aaron Blaine , Aschner Michael
Published: September 2019

Manganese (Mn) is an essential trace element that is naturally found in the environment and is necessary as a cofactor for many enzymes and is important in several physiological processes that support development, growth, and neuronal function. However, overexposure to Mn may induce neurotoxicity and may contribute to the development of Alzheimer’s disease (AD) and Parkinson’s disease (PD). The present review aims to provide new insights into the involvement of Mn in the etiology of AD and PD. Here, we discuss the critical role of Mn in the etiology of these disorders and provide a summary of the proposed mechanisms underlying Mn-induced neurodegeneration. In addition, we review some new therapy options for AD and PD related to Mn overload.
Associations Between Sub-Clinical Markers of Cardiometabolic Risk and Exposure to Residential Indoor Air Pollutants in Healthy Adults in Perth, Western Australia: A Study Protocol
Authors: Gilbey Suzanne E. , Reid Christopher M. , Huxley Rachel R. , Soares Mario J. , Zhao Yun , Rumchev Krassi
Published: September 2019

Background: A growing body of epidemiological and clinical evidence has implicated air pollution as an emerging risk factor for cardiometabolic disease. Whilst individuals spend up to two-thirds of daily time in their domestic residential environment, very few studies have been designed to objectively measure the sub-clinical markers of cardiometabolic risk with exposure to domestic indoor air pollutants. This cross-sectional study aims to investigate associations between the components of domestic indoor air quality and selected sub-clinical cardiometabolic risk factors in a cohort of healthy adults living in Perth, Western Australia. Methods: One hundred and eleven non-smoking adults (65% female) living in non-smoking households who were aged between 35–69 years were recruited for the project. Study subjects were invited to participate in all sections of the study, which included: Domestic indoor air monitoring along with the concurrent 24 h ambulatory monitoring of peripheral and central blood pressure and measures of central hemodynamic indices, standardized questionnaires on aspects relating to current health status and the domestic environment, a 24 h time-activity diary during the monitoring period, and clinic-based health assessment involving collection of blood and urine biomarkers for lipid and glucose profiles, as well as measures of renal function and an analysis of central pulse wave and pulse wave velocity. Results: This study provides a standardized approach to the study of sub-clinical cardiometabolic health effects that are related to the exposure to indoor air pollution. Conclusion: The findings of this study may provide direction for future research that will further contribute to our understanding of the relationship that exists between indoor air pollution and sub-clinical markers of cardiometabolic risk.
A Qualitative Study on Employees’ Experiences of a Support model for Systematic Work Environment Management
Authors: Hellman Therese , Molin Fredrik , Svartengren Magnus
Published: September 2019

Background: The aim is to explore how an organisational work environment support model, the Stamina model, influences employees’ work situations and the development of sustainable work systems. Methods: It was a qualitative study with semi-structured, focus-group interviews, including 45 employees from six work groups. Eighteen focus group interviews were conducted over a period of two years. Data were analysed with constant comparative method. Results: The core category, shifting focus from an individual to an organisational perspective of work, illustrated how communication and increased understanding of one’s work tasks changed over time and contributed to deeper focus on the actual operation. These insights were implemented at different time points among the work groups during the two-year process. Conclusions: Our results indicate that working with the model engages employees in the work environment management, puts emphasis on reflections and discussions about the meaning and purpose of the operations and enables a shared platform for communication. These are important features that need to continue over time in order to create a sustainable work system. The Stamina model, thus seems to have the potential to promote productive and healthy work places.
Evidence of Workplace Interventions—A Systematic Review of Systematic Reviews
Authors: Pieper Claudia , Schröer Sarah , Eilerts Anna-Lisa
Published: September 2019

Work environment factors are highly correlated with employees’ health and well-being. Our aim was to sum up current evidence of health promotion interventions in the workplace, focusing on interventions for the prevention of musculoskeletal disorders, psychological and behavioral disorders as well as interventions for older employees and economic evaluations. We conducted a comprehensive literature search including systematic reviews published from April 2012 to October 2017 in electronic databases and search engines, websites of relevant organizations and institutions. It consisted of simple and specific terms and word combinations related to workplace health promotion based on the search strategy of a previous review. After full-text screening, 74 references met the eligibility criteria. Using the same search strategy, there was a higher proportion of relevant high-quality studies as compared with the earlier review. The heterogeneity of health promotion interventions regarding intervention components, settings and study populations still limits the comparability of studies. Future studies should also address the societal and insurer perspective, including costs to the worker such as lost income and lost time at work of family members due to caregiving activities. To this end, more high-quality evidence is needed.
Residents’ Diachronic Perception of the Impacts of Ecological Resettlement in a World Heritage Site
Authors: Wang Kai , Wang Menghan , Gan Chang , Voda Mihai
Published: September 2019

As one of the main factors in any tourist destination, residents’ perception of the impacts of ecological resettlement has a substantial influence on the sustainable development of any world heritage site. Our research takes the residents of three different resettlement locations in the Wulingyuan scenic area, a world heritage site, as the object of our survey. Based on questionnaire investigations in 2010 and 2016, this article analyzes the residents’ diachronic perception of the impacts of ecological resettlement. Independent sample t-tests and Analysis of Variance (ANOVA) are used to compare the differences in residents’ perception toward ecological relocation and analyse how demographic characteristics affect residents’ perception. Multiple stepwise regression analysis is applied to explore the main factors that contribute to the differences in the perception of impacts of ecological resettlement. The results show that during the study period, respondents have the strongest perceptions of the economic, socio-cultural, resource-environment and psychological impacts. However, they have negative perceptions of relocation policy impacts. Compared with 2010, residents with different gender, age, education level, income level and engagement in tourism have significant differences in perception of impacts of resettlement in 2016. Multiple stepwise regression analysis demonstrates that the perceptions of impacts of the ecological resettlement and economic policy are the primary factors to affect residents’ overall perceptions.
Geochemical Evidence of Metal-Driven Anaerobic Oxidation of Methane in the Shenhu Area, the South China Sea
Authors: Xie Rui , Wu Daidai , Liu Jie , Sun Tiantian , Liu Lihua , Wu Nengyou
Published: September 2019

Anaerobic oxidation of methane (AOM) is a common biochemical process in the ocean and it plays an important role in global climate change, elemental circulation, and atmospheric evolution over geological time. In this paper, we analyzed of δ34S, Fe, Mn, Ca/Ti, and Sr/Ti ratios, and the date of carbon and sulfur from the site SH3 of Shenhu area. Result showed that (1) 0–6 mbsf (meter blow the sea floor) was mainly affected by OSR (anaerobic oxidation of organic matters) and 7–15 mbsf was a paleo-SMTZ (sulfate–methane transition zone) position. The modern SMTZ was mainly distributed at 19–25 mbsf. The barium sulfate precipitation above the modern SMTZ indicating that the current methane leakage was stable and lasted longer during geological history. (2) By studying the change of magnetic and the different carbonate minerals, results showed that there were two AOM stages. During the early stage, Fe2+ were mainly produced by sulfide abiotic reductive dissolution. During the later stage, Fe2+ were mainly produced by the metal-AOM. (3) Study of the mineral characteristics of the paleo-SMTZ and the modern SMTZ showed that the modern SMTZ carbonate minerals were mainly low-Mg calcite and aragonite, while the paleo-SMTZ carbon minerals were mainly high Mg minerals. The reason for this difference is that the modern SMTZ layer was only experienced the first stage of anaerobic oxidation of methane. In the paleo-SMTZ layer, it has experienced two stage of anaerobic oxidation of methane. During the last stage of metal-AOM, the low Mg carbonate minerals were converted into high Mg carbonate minerals. This research confirms the presence of metal-driven methane anaerobic oxidation at the bottom of sulfate-driven methane anaerobic oxidation and during the metal-driven methane anaerobic oxidation, methane and metal oxides or hydroxides would couple to convert the in situ metal oxides or hydroxides into metal ions, meanwhile the phosphorus adsorbed on the surface of the metal oxides is released into adjacent pore water, and convert to new P-bearing minerals under suitable conditions.
Quality of Life and Associated Factors among Primary Care Asian Patients with Type 2 Diabetes Mellitus
Authors: Dhillon Hardesh , Nordin Rusli Bin , Ramadas Amutha
Published: September 2019

Diabetes complications, medication adherence, and psychosocial well-being have been associated with quality of life (QOL) among several Western and Asian populations with diabetes, however, there is little evidence substantiating these relationships among Malaysia’s unique and diverse population. Therefore, a cross-sectional study was conducted in a Malaysian public primary care clinic among 150 patients diagnosed with type 2 diabetes mellitus (T2DM). Structured and validated questionnaires were used to investigate the associations between demographic, clinical, and psychological factors with QOL of the study participants. Approximately three-quarters of patients had a good-excellent QOL. Diabetes-related variables that were significantly associated with poor QOL scores included insulin containing treatment regimens, poor glycemic control, inactive lifestyle, retinopathy, neuropathy, abnormal psychosocial well-being, higher diabetes complication severity, and nonadherence (p < 0.05). The main predictors of a good-excellent QOL were HbA1c ≤ 6.5% (aOR = 20.78, 95% CI = 2.5175.9, p = 0.005), normal anxiety levels (aOR = 5.73, 95% CI = 1.8–18.5, p = 0.004), medication adherence (aOR = 3.35, 95% CI = 1.3–8.7, p = 0.012), and an aDCSI score of one and two as compared to those greater than or equal to four (aOR = 7.78, 95% CI = 1.5–39.2, p = 0.013 and aOR = 8.23, 95% CI = 2.1–32.8, p = 0.003), respectively. Medication adherence has also been found to be an effect modifier of relationships between HbA1c, depression, anxiety, disease severity, and QOL. These predictors of QOL are important factors to consider when managing patients with T2DM.
Comparison of Whole Plastome Sequences between Thermogenic Skunk Cabbage Symplocarpus renifolius and Nonthermogenic S. nipponicus (Orontioideae; Araceae) in East Asia
Authors: Kim Seon-Hee , Yang JiYoung , Park Jongsun , Yamada Takayuki , Maki Masayuki , Kim Seung-Chul
Published: September 2019

Symplocarpus, a skunk cabbage genus, includes two sister groups, which are drastically different in life history traits and thermogenesis, as follows: The nonthermogenic summer flowering S. nipponicus and thermogenic early spring flowering S. renifolius. Although the molecular basis of thermogenesis and complete chloroplast genome (plastome) of thermogenic S. renifolius have been well characterized, very little is known for that of S. nipponicus. We sequenced the complete plastomes of S. nipponicus sampled from Japan and Korea and compared them with that of S. renifolius sampled from Korea. The nonthermogenic S. nipponicus plastomes from Japan and Korea had 158,322 and 158,508 base pairs, respectively, which were slightly shorter than the thermogenic plastome of S. renifolius. No structural or content rearrangements between the species pairs were found. Six highly variable noncoding regions (psbC/trnS, petA/psbJ, trnS/trnG, trnC/petN, ycf4/cemA, and rpl3/rpl22) were identified between S. nipponicus and S. renifolius and 14 hot-spot regions were also identified at the subfamily level. We found a similar total number of SSR (simple sequence repeat) motifs in two accessions of S. nipponicus sampled from Japan and Korea. Phylogenetic analysis supported the basal position of subfamily Orontioideae and the monophyly of genus Symplocarpus, and also revealed an unexpected evolutionary relationship between S. nipponicus and S. renifolius.
Unique Features of Entamoeba Sulfur Metabolism; Compartmentalization, Physiological Roles of Terminal Products, Evolution and Pharmaceutical Exploitation
Authors: Mi-ichi Fumika , Yoshida Hiroki
Published: September 2019

Sulfur metabolism is essential for all living organisms. Recently, unique features of the Entamoeba metabolic pathway for sulfated biomolecules have been described. Entamoeba is a genus in the phylum Amoebozoa and includes the causative agent for amoebiasis, a global public health problem. This review gives an overview of the general features of the synthesis and degradation of sulfated biomolecules, and then highlights the characteristics that are unique to Entamoeba. Future biological and pharmaceutical perspectives are also discussed.
Short-Time Ocular Ischemia Induces Vascular Endothelial Dysfunction and Ganglion Cell Loss in the Pig Retina
Authors: Zadeh Jenia Kouchek , Garcia-Bardon Andreas , Hartmann Erik Kristoffer , Pfeiffer Norbert , Omran Wael , Ludwig Marion , Patzak Andreas , Xia Ning , Li Huige , Gericke Adrian
Published: September 2019

Visual impairment and blindness are often caused by retinal ischemia-reperfusion (I/R) injury. We aimed to characterize a new model of I/R in pigs, in which the intraocular pathways were not manipulated by invasive methods on the ocular system. After 12 min of ischemia followed by 20 h of reperfusion, reactivity of retinal arterioles was measured in vitro by video microscopy. Dihydroethidium (DHE) staining, qPCR, immunohistochemistry, quantification of neurons in the retinal ganglion cell layer, and histological examination was performed. Retinal arterioles of I/R-treated pigs displayed marked attenuation in response to the endothelium-dependent vasodilator, bradykinin, compared to sham-treated pigs. DHE staining intensity and messenger RNA levels for HIF-1α, VEGF-A, NOX2, and iNOS were elevated in retinal arterioles following I/R. Immunoreactivity to HIF-1α, VEGF-A, NOX2, and iNOS was enhanced in retinal arteriole endothelium after I/R. Moreover, I/R evoked a substantial decrease in Brn3a-positive retinal ganglion cells and noticeable retinal thickening. In conclusion, the results of the present study demonstrate that short-time ocular ischemia impairs endothelial function and integrity of retinal blood vessels and induces structural changes in the retina. HIF-1α, VEGF-A, iNOS, and NOX2-derived reactive oxygen species appear to be involved in the pathophysiology.
Chloride as a Beneficial Macronutrient in Higher Plants: New Roles and Regulation
Authors: Colmenero-Flores José M. , Franco-Navarro Juan D. , Cubero-Font Paloma , Peinado-Torrubia Procopio , Rosales Miguel A.
Published: September 2019

Chloride (Cl−) has traditionally been considered a micronutrient largely excluded by plants due to its ubiquity and abundance in nature, its antagonism with nitrate (NO3−), and its toxicity when accumulated at high concentrations. In recent years, there has been a paradigm shift in this regard since Cl− has gone from being considered a harmful ion, accidentally absorbed through NO3− transporters, to being considered a beneficial macronutrient whose transport is finely regulated by plants. As a beneficial macronutrient, Cl− determines increased fresh and dry biomass, greater leaf expansion, increased elongation of leaf and root cells, improved water relations, higher mesophyll diffusion to CO2, and better water- and nitrogen-use efficiency. While optimal growth of plants requires the synchronic supply of both Cl− and NO3− molecules, the NO3−/Cl− plant selectivity varies between species and varieties, and in the same plant it can be modified by environmental cues such as water deficit or salinity. Recently, new genes encoding transporters mediating Cl− influx (ZmNPF6.4 and ZmNPF6.6), Cl− efflux (AtSLAH3 and AtSLAH1), and Cl− compartmentalization (AtDTX33, AtDTX35, AtALMT4, and GsCLC2) have been identified and characterized. These transporters have proven to be highly relevant for nutrition, long-distance transport and compartmentalization of Cl−, as well as for cell turgor regulation and stress tolerance in plants.
The Role of Exo-miRNAs in Cancer: A Focus on Therapeutic and Diagnostic Applications
Authors: Ingenito Francesco , Roscigno Giuseppina , Affinito Alessandra , Nuzzo Silvia , Scognamiglio Iolanda , Quintavalle Cristina , Condorelli Gerolama
Published: September 2019

Exosomes are extracellular vesicles released into biological fluids where they act as carriers of various molecules, including proteins, lipids, and RNAs, between cells, modulating or perturbing specific physiological processes. Recently, it has been suggested that tumoral cells release excessive amounts of exosomes that, through their cargo, promote tumor progression, stimulating growth, angiogenesis, metastasis, insensitivity to chemotherapy, and immune evasion. Increasing evidence highlights exosomal microRNAs (exo-miRNAs) as important players in tumorigenesis. MicroRNA (miRNA) are a class of small non-coding RNA able to regulate gene expression, targeting multiple mRNAs and inducing translational repression and/or mRNA degradation. Exo-miRNAs are highly stable and easily detectable in biological fluids, and for these reasons, miRNAs are potential cancer biomarkers useful diagnostically and prognostically. Furthermore, since exosomes are natural delivery systems between cells, they can be appropriately modified to carry therapeutic miRNAs to specific recipient cells. Here we summarize the main functions of exo-miRNAs and their possible role for diagnostic and therapeutic applications.
Knockdown of RhoE Expression Enhances TGF-β-Induced EMT (epithelial-to-mesenchymal transition) in Cervical Cancer HeLa Cells
Authors: Nishizuka Makoto , Komada Rina , Imagawa Masayoshi
Published: September 2019

Cervical cancer with early metastasis of the primary tumor is associated with poor prognosis and poor therapeutic outcomes. Since epithelial-to-mesenchymal transition (EMT) plays a role in acquisition of the ability to invade the pelvic lymph nodes and surrounding tissue, it is important to clarify the molecular mechanism underlying EMT in cervical cancer. RhoE, also known as Rnd3, is a member of the Rnd subfamily of Rho GTPases. While previous reports have suggested that RhoE may act as either a positive or a negative regulator of cancer metastasis and EMT, the role of RhoE during EMT in cervical cancer cells remains unclear. The present study revealed that RhoE expression was upregulated during transforming growth factor-β (TGF-β)-mediated EMT in human cervical cancer HeLa cells. Furthermore, reduced RhoE expression enhanced TGF-β-mediated EMT and migration of HeLa cells. In addition, we demonstrated that RhoE knockdown elevated RhoA activity and a ROCK inhibitor partially suppressed the acceleration of TGF-β-mediated EMT by RhoE knockdown. These results indicate that RhoE suppresses TGF-β-mediated EMT, partially via RhoA/ROCK signaling in cervical cancer HeLa cells.
Genome-Wide Analysis and Identification of the Aux/IAA Gene Family in Peach
Authors: Guan Dan , Hu Xiao , Diao Donghui , Wang Fang , Liu Yueping
Published: September 2019

The Auxin/indole-3-acetic acid (Aux/IAA) repressor genes down-regulate the auxin response pathway during many stages of plant and fruit development. In order to determine if and how Aux/IAAs participate in governing texture and hardness in stone fruit maturation, we identified 23 Aux/IAA genes in peach, confirmed by the presence of four conserved domains. In this work, we used fluorescence microscopy with PpIAA-GFP fusion reporters to observe their nuclear localization. We then conducted PCR-based differential expression analysis in “melting” and “stony hard” varieties of peach, and found that in the “melting” variety, nine PpIAAs exhibited peak expression in the S4-3 stage of fruit maturation, with PpIAA33 showing the highest (>120-fold) induction. The expression of six PpIAAs peaked in the S4-2 stage, with PpIAA14 expressed the most highly. Only PpIAA15/16 showed higher expression in the “stony hard” variety than in the “melting” variety, both peaking in the S3 stage. In contrast, PpIAA32 had the highest relative expression in buds, flowers, young and mature leaves, and roots. Our study provides insights into the expression patterns of Aux/IAA developmental regulators in response to auxin during fruit maturation, thus providing insight into their potential development as useful markers for quantitative traits associated with fruit phenotype.
Activating Hippo Pathway via Rassf1 by Ursolic Acid Suppresses the Tumorigenesis of Gastric Cancer
Authors: Kim Seong-Hun , Jin Hua , Meng Ruo Yu , Kim Da-Yeah , Liu Yu Chuan , Chai Ok Hee , Park Byung Hyun , Kim Soo Mi
Published: September 2019

The Hippo pathway is often dysregulated in many carcinomas, which results in various stages of tumor progression. Ursolic acid (UA), a natural compound that exists in many herbal plants, is known to obstruct cancer progression and exerts anti-carcinogenic effect on a number of human cancers. In this study, we aimed to examine the biological mechanisms of action of UA through the Hippo pathway in gastric cancer cells. MTT assay showed a decreased viability of gastric cancer cells after treatment with UA. Following treatment with UA, colony numbers and the sizes of gastric cancer cells were significantly diminished and apoptosis was observed in SNU484 and SNU638 cells. The invasion and migration rates of gastric cancer cells were suppressed by UA in a dose-dependent manner. To further determine the gene expression patterns that are related to the effects of UA, a microarray analysis was performed. Gene ontology analysis revealed that several genes, such as the Hippo pathway upstream target gene, ras association domain family (RASSF1), and its downstream target genes (MST1, MST2, and LATS1) were significantly upregulated by UA, while the expression of YAP1 gene, together with oncogenes (FOXM1, KRAS, and BATF), were significantly decreased. Similar to the gene expression profiling results, the protein levels of RASSF1, MST1, MST2, LATS1, and p-YAP were increased, whereas those of CTGF were decreased by UA in gastric cancer cells. The p-YAP expression induced in gastric cancer cells by UA was reversed with RASSF1 silencing. In addition, the protein levels in the Hippo pathway were increased in the UA-treated xenograft tumor tissues as compared with that in the control tumor tissues; thus, UA significantly inhibited the tumorigenesis of gastric cancer in vivo in xenograft animals. Collectively, UA diminishes the proliferation and metastasis of gastric cancer via the regulation of Hippo pathway through Rassf1, which suggests that UA can be used as a potential chemopreventive and therapeutic agent for gastric cancer.