bluuebunny/crossref_metadata_2025 huggingface dataset:
Geometric Morphometric Versus Genomic Patterns in a Large Polyploid Plant Species Complex
Authors: Hodač Ladislav , Karbstein Kevin , Tomasello Salvatore , Wäldchen Jana , Bradican John Paul , Hörandl Elvira
Published: March 2023

Plant species complexes represent a particularly interesting example of taxonomically complex groups (TCGs), linking hybridization, apomixis, and polyploidy with complex morphological patterns. In such TCGs, mosaic-like character combinations and conflicts of morphological data with molecular phylogenies present a major problem for species classification. Here, we used the large polyploid apomictic European Ranunculus auricomus complex to study relationships among five diploid sexual progenitor species and 75 polyploid apomictic derivate taxa, based on geometric morphometrics using 11,690 landmarked objects (basal and stem leaves, receptacles), genomic data (97,312 RAD-Seq loci, 48 phased target enrichment genes, 71 plastid regions) from 220 populations. We showed that (1) observed genomic clusters correspond to morphological groupings based on basal leaves and concatenated traits, and morphological groups were best resolved with RAD-Seq data; (2) described apomictic taxa usually overlap within trait morphospace except for those taxa at the space edges; (3) apomictic phenotypes are highly influenced by parental subgenome composition and to a lesser extent by climatic factors; and (4) allopolyploid apomictic taxa, compared to their sexual progenitor, resemble a mosaic of ecological and morphological intermediate to transgressive biotypes. The joint evaluation of phylogenomic, phenotypic, reproductive, and ecological data supports a revision of purely descriptive, subjective traditional morphological classifications.
Antibiotic Resistance, Virulence Gene Detection, and Biofilm Formation in Aeromonas spp. Isolated from Fish and Humans in Egypt
Authors: El-Hossary Dalia , Mahdy Asmaa , Elariny Eman Y. T. , Askora Ahmed , Merwad Abdallah M. A. , Saber Taisir , Dahshan Hesham , Hakami Nora Y. , Ibrahim Rehab A.
Published: March 2023

The genus Aeromonas is widely distributed in aquatic environments and is recognized as a potential human pathogen. Some Aeromonas species are able to cause a wide spectrum of diseases, mainly gastroenteritis, skin and soft-tissue infections, bacteremia, and sepsis. The aim of the current study was to determine the prevalence of Aeromonas spp. in raw fish markets and humans in Zagazig, Egypt; identify the factors that contribute to virulence; determine the isolates’ profile of antibiotic resistance; and to elucidate the ability of Aeromonas spp. to form biofilms. The examined samples included fish tissues and organs from tilapia (Oreochromis niloticus, n = 160) and mugil (Mugil cephalus, n = 105), and human skin swabs (n = 51) and fecal samples (n = 27). Based on biochemical and PCR assays, 11 isolates (3.2%) were confirmed as Aeromonas spp. and four isolates (1.2%) were confirmed as A. hydrophila. The virulence genes including haemolysin (hyl A) and aerolysin (aer) were detected using PCR in A. hydrophila in percentages of 25% and 50%, respectively. The antimicrobial resistance of Aeromonas spp. was assessed against 14 antibiotics comprising six classes. The resistance to cefixime (81.8%) and tobramycin (45.4%) was observed. The multiple antibiotic resistance (MAR) index ranged between 0.142–0.642 with 64.2% of the isolates having MAR values equal to 0.642. Biofilm formation capacity was assessed using a microtiter plate assay, and two isolates (18.1%) were classified as biofilm producers. This study establishes a baseline for monitoring and controlling the multidrug-resistant Aeromonas spp. and especially A. hydrophila in marine foods consumed in our country to protect humans and animals.
Evaluation of Thermal Changes of the Sole Surface in Horses with Palmar Foot Pain: A Pilot Study
Authors: Zaha Cristian , Schuszler Larisa , Dascalu Roxana , Nistor Paula , Florea Tiana , Imre Kálmán , Rujescu Ciprian , Sicoe Bogdan , Igna Cornel
Published: March 2023

Background: Horses with palmar foot pain do not show a typical increase in temperature in the palmar aspect of the hoof and heel due to low blood flow. The objectives of the current study were to determine the changes and differences in the thermographic pattern of the sole surface in horses with unilateral palmar foot pain and non-lame horses before and after training. We hypothesized that the thermal pattern and the local temperature of the frog area and toe area would be similar, with an increased local temperature after training in both lame and non-lame horses. A pilot study was conducted to investigate the accuracy of thermography used to detect changes in local temperature and to compare the thermal patterns observed on the sole surface after training. Methods: The study group included eight horses with unilateral forelimb palmar foot pain (n = 8) and healthy contralateral limbs (n = 8). Four additional non-lame horses served as controls. The horses were selected for the study based on the following criteria: forelimb with unilateral chronic progressive lameness and positive reactions when performing the hoof test and frog wedge test, degenerative findings of the navicular bone, and improvement in lameness after perineural analgesia of the medial and lateral palmar digital nerve. The local temperatures of the frog and toe areas were measured before and after training in the affected and contralateral limbs in the study group and both forelimbs in the control group using an FLIR E50 thermal camera. Receiver operating characteristic (ROC) curve analysis was applied to check the sensitivity and specificity of the results for the studied groups. Results: The thermal patterns of the hoof presented differences between the three groups of horse limbs. After training, the temperature of the sole surface increased, and its area increased in the limbs affected with palmar foot pain compared with the non-lame limbs in the study group and the limbs in the control group. The temperature of the frog area did not increase after training in the limbs affected with palmar foot pain compared with the same area in the other groups. The ROC curve analysis demonstrated the medical applicability of this tool and showed that thermography is a reliable diagnostic test to correctly discriminate between non-lame horses and those with palmar foot pain. Conclusions: We recommend thermographic scanning of the toe and frog area to detect changes in the local temperature of the sole surface after training to discriminate the horses with palmar foot pain from non-lame ones. Further investigation is required to clarify whether the observed thermographic imaging features of the sole surface are characteristic for horses with palmar foot pain.
Swimming Behavior of Daphnia magna Is Altered by Pesticides of Concern, as Components of Agricultural Surface Water and in Acute Exposures
Authors: Egan Nicole , Stinson Sarah A. , Deng Xin , Lawler Sharon P. , Connon Richard E.
Published: March 2023

Pesticides with novel modes of action including neonicotinoids and anthranilic diamides are increasingly detected in global surface waters. Little is known about how these pesticides of concern interact in mixtures at environmentally relevant concentrations, a common exposure scenario in waterways impacted by pesticide pollution. We examined effects of chlorantraniliprole (CHL) and imidacloprid (IMI) on the sensitive invertebrate, Daphnia magna. Exposures were first performed using surface waters known to be contaminated by agricultural runoff. To evaluate the seasonal variation in chemical concentration and composition of surface waters, we tested surface water samples taken at two time points: during an extended dry period and after a first flush storm event. In surface waters, the concentrations of CHL, IMI, and other pesticides of concern increased after first flush, resulting in hypoactivity and dose-dependent photomotor responses. We then examined mortality and behavior following single and binary chemical mixtures of CHL and IMI. We detected inverse photomotor responses and some evidence of synergistic effects in binary mixture exposures. Taken together, this research demonstrates that CHL, IMI, and contaminated surface waters all cause abnormal swimming behavior in D. magna. Invertebrate swimming behavior is a sensitive endpoint for measuring the biological effects of environmental pesticides of concern.
Genome-Wide Identification and Expression Analysis of the TIR-NBS-LRR Gene Family and Its Response to Fungal Disease in Rose (Rosa chinensis)
Authors: Song Jurong , Chen Feng , Lv Bo , Guo Cong , Yang Jie , Huang Li , Guo Jiaqi , Xiang Fayun
Published: March 2023

Roses, which are one of the world’s most important ornamental plants, are often damaged by pathogens, resulting in serious economic losses. As a subclass of the disease resistance gene family of plant nucleotide-binding oligomerization domain (NOD)-like receptors, TIR-NBS-LRR (TNL) genes play a vital role in identifying pathogen effectors and activating defense responses. However, a systematic analysis of the TNL gene family is rarely reported in roses. Herein, 96 intact TNL genes were identified in Rosa chinensis. Their phylogenies, physicochemical characteristics, gene structures, conserved domains and motifs, promoter cis-elements, microRNA binding sites, and intra- and interspecific collinearity relationships were analyzed. An expression analysis using transcriptome data revealed that RcTNL genes were dominantly expressed in leaves. Some RcTNL genes responded to gibberellin, jasmonic acid, salicylic acid, Botrytis cinerea, Podosphaera pannosa, and Marssonina rosae (M. rosae); the RcTNL23 gene responded significantly to three hormones and three pathogens, and exhibited an upregulated expression. Furthermore, the black spot pathogen was identified as M. rosae. After inoculating rose leaves, an expression pattern analysis of the RcTNL genes suggested that they act during different periods of pathogen infection. The present study lays the foundations for an in-depth investigation of the TNL gene function and the mining of disease resistance genes in roses.
Molecular Property Prediction by Combining LSTM and GAT
Authors: Xu Lei , Pan Shourun , Xia Leiming , Li Zhen
Published: March 2023

Molecular property prediction is an important direction in computer-aided drug design. In this paper, to fully explore the information from SMILE stings and graph data of molecules, we combined the SALSTM and GAT methods in order to mine the feature information of molecules from sequences and graphs. The embedding atoms are obtained through SALSTM, firstly using SMILES strings, and they are combined with graph node features and fed into the GAT to extract the global molecular representation. At the same time, data augmentation is added to enlarge the training dataset and improve the performance of the model. Finally, to enhance the interpretability of the model, the attention layers of both models are fused together to highlight the key atoms. Comparison with other graph-based and sequence-based methods, for multiple datasets, shows that our method can achieve high prediction accuracy with good generalizability.
Exploring the Potential of the Model Cyanobacteria Synechococcus PCC 7002 and PCC 7942 for the Photoproduction of High-Value Terpenes: A Comparison with Synechocystis PCC 6803
Authors: Chenebault Célia , Blanc-Garin Victoire , Vincent Marine , Diaz-Santos Encarnación , Goudet Amélie , Cassier-Chauvat Corinne , Chauvat Franck
Published: March 2023

We have performed the first comparative analysis of the potential of two physiologically-diverse model cyanobacteria, Synechococcus PCC 7002 (S.7002) and Synechococcus elongatus PCC 7942 (S.7942), for the photosynthetic production of four chemically-different high-value terpenes: two monoterpenes limonene and pinene, and two sesquiterpenes bisabolene and farnesene. We showed, for the first time, that S.7002 and S.7942 can produce farnesene and bisabolene, respectively. Both cyanobacteria produced farnesene (S.7942 produced more efficiently than S.7002) more efficiently than the other tested terpenes (especially pinene, the weakest produced terpene). S.7002 produced limonene more efficiently than bisabolene, whereas S.7942 produced bisabolene more efficiently than limonene. These findings suggest that S.7942 is better suited to produce sesquiterpenes than monoterpenes. Interestingly, higher levels of terpenes were produced by S.7942 and S.7002 expressing a terpene-synthase gene from both an RSF1010-derived replicating plasmid and a neutral chromosomal site, as compared to either the plasmid alone or the chromosome alone. These results suggest that in both cyanobacteria, the production of terpenes is more limited by the activity of terpene synthases than the abundance of terpene precursors. Finally, higher levels of terpenes were produced by S.7002 growing on urea (a frequent pollutant) as compared to nitrate or ammonium, the standard nitrogen sources for cyanobacteria.
Gap Junctions and Connexins in Microglia-Related Oxidative Stress and Neuroinflammation: Perspectives for Drug Discovery
Authors: Caruso Giuseppe , Di Pietro Lucia , Caraci Filippo
Published: March 2023

Microglia represent the immune system of the brain. Their role is central in two phenomena, neuroinflammation and oxidative stress, which are at the roots of different pathologies related to the central nervous system (CNS). In order to maintain the homeostasis of the brain and re-establish the equilibrium after a threatening imbalance, microglia communicate with each other and other cells within the CNS by receiving specific signals through membrane-bound receptors and then releasing neurotrophic factors into either the extracellular milieu or directly into the cytoplasm of nearby cells, such as astrocytes and neurons. These last two mechanisms rely on the activity of protein structures that enable the formation of channels in the membrane, namely, connexins and pannexins, that group and form gap junctions, hemichannels, and pannexons. These channels allow the release of gliotransmitters, such as adenosine triphosphate (ATP) and glutamate, together with calcium ion (Ca2+), that seem to play a pivotal role in inter-cellular communication. The aim of the present review is focused on the physiology of channel protein complexes and their contribution to neuroinflammatory and oxidative stress-related phenomena, which play a central role in neurodegenerative disorders. We will then discuss how pharmacological modulation of these channels can impact neuroinflammatory phenomena and hypothesize that currently available nutraceuticals, such as carnosine and N-acetylcysteine, can modulate the activity of connexins and pannexins in microglial cells and reduce oxidative stress in neurodegenerative disorders.
p53 Function and Dysfunction in Human Health and Diseases
Authors: D’Orazi Gabriella
Published: March 2023

The p53 protein is a sequence-specific DNA-binding transcription factor that, in response to stressful stimuli, regulates gene expression related to multiple cellular functions including, but not limited to, cell cycle arrest, cell apoptosis, cell growth, DNA repair, cell metabolism, and the immune response [...]
Association of Serum Vaspin Concentration with Metabolic Disorders in Obese Individuals
Authors: Pilarski Łukasz , Pelczyńska Marta , Koperska Anna , Seraszek-Jaros Agnieszka , Szulińska Monika , Bogdański Paweł
Published: March 2023

Vaspin, a molecule produced in visceral adipose tissue, seems to participate in the pathogenesis of metabolic disorders. The study aimed to determine the association of vaspin concentration with metabolic disorders in obese individuals. Forty obese patients and twenty normal-weight subjects underwent biochemical (fasting glucose, insulin, lipid profile, interleukin-6, hs-CRP, vaspin concentration), blood pressure, and anthropometric measurements. The HOMA-IR index was calculated. Serum vaspin concentrations in the obese group were significantly higher than in the control group (0.82 ± 0.62 vs. 0.43 ± 0.59; p < 0.001). Among the entire population, vaspin concentration was positively correlated with body weight, BMI, WHR, and the percentage and mass of adipose tissue. Positive correlations between vaspin concentration and triglyceride level, insulin concentration, and HOMA-IR value were found. Vaspin concentration was positively correlated with hs-CRP and IL-6 levels. In obese patients, positive correlations between vaspin concentration and the percentage of adipose tissue and hs-CRP level were demonstrated. Logistic regression analysis showed that increased BMI was the biggest factor stimulating vaspin concentrations (OR = 8.5; 95% CI: 1.18–61.35; p = 0.0338). An elevated vaspin level may imply its compensatory role against metabolic disorders in obese patients. Thus, vaspin appears to be a useful diagnostic parameter for new therapeutic approaches in obesity-related complications. Nevertheless, due to the small sample size, further studies are needed to confirm our results.
Carnosic Acid Ameliorates Indomethacin-Induced Gastric Ulceration in Rats by Alleviating Oxidative Stress and Inflammation
Authors: Danisman Betul , Cicek Betul , Yildirim Serkan , Bolat Ismail , Kantar Deniz , Golokhvast Kirill S. , Nikitovic Dragana , Tsatsakis Aristidis , Taghizadehghalehjoughi Ali
Published: March 2023

Nonsteroidal anti-inflammatory drugs (NSAIDs) such as aspirin and indomethacin (IND) are the most commonly prescribed for inflammation or pain. However, widespread use causes several adverse effects, such as gastric ulcers, upper gastric system bleeding, and erosions. Carnosic acid (CA) is an important natural antioxidant found in rosemary (Rosmarinus essentials) and exhibits a protective effect by suppressing oxidative stress and inflammation. This study aimed to investigate the impact of CA on IND-induced gastric ulceration. Wistar male rats received CA (100 mg/kg) or esomeprazole (ESP) (20 mg/kg, standard drug) by oral gavage for 14 days, after that gastric ulceration was induced by oral administration of 100 mg/kg IND. CA pretreatment attenuated both gross morphological lesions and histopathological alterations. CA strongly reduced IND-induced oxidative stress, verified by a decrease in MDA (p < 0.001) and TOS levels (p < 0.05). Furthermore, an IND-dependent increase in CAT (p < 0.001) and GPx (p < 0.01) activities, as well as a reduction in GSH levels (p < 0.01), were ameliorated by CA pretreatment. CA also attenuated inflammatory damage by suppressing IL-1β (p < 0.01), IL-6 (p < 0.01), and TNFα (p < 0.001) production and increasing Nrf2/HO-1 (p < 0.05) expressions. In conclusion, CA shows a gastroprotective effect by reducing oxidative stress and attenuating inflammation.
A Brief Review of Duchenne Muscular Dystrophy Treatment Options, with an Emphasis on Two Novel Strategies
Authors: Heydemann Ahlke , Siemionow Maria
Published: March 2023

Despite the full cloning of the Dystrophin cDNA 35 years ago, no effective treatment exists for the Duchenne Muscular Dystrophy (DMD) patients who have a mutation in this gene. Many treatment options have been considered, investigated preclinically and some clinically, but none have circumvented all barriers and effectively treated the disease without burdening the patients with severe side-effects. However, currently, many novel therapies are in the pipelines of research labs and pharmaceutical companies and many of these have progressed to clinical trials. A brief review of these promising therapies is presented, followed by a description of two novel technologies that when utilized together effectively treat the disease in the mdx mouse model. One novel technology is to generate chimeric cells from the patient’s own cells and a normal donor. The other technology is to systemically transplant these cells into the femur via the intraosseous route.
Buffy Coat Processing Impacts on Monocytes’ Capacity to Present Lipid Antigens
Authors: Mondragão-Rodrigues Inês , Macedo M. Fátima
Published: March 2023

Buffy Coats, generated from a blood donor’s whole blood bag unit, are commonly used in biomedical research as a source of leukocytes due to the high number of cells that can be recovered from each Buffy Coat. Buffy Coats are leukocyte-enriched residual units obtained by centrifugation of whole blood. At the blood bank, blood can be processed using two different protocols according to the time interval between blood collection and processing. When blood collection and processing occur on the same day, it gives rise to Fresh Blood Buffy Coats. Alternatively, if blood processing only happens on the day after blood collection, Overnight Blood Buffy Coats are created. In this study, we aimed to address whether these two different Buffy Coat-processing protocols could differently impact monocyte function as antigen-presenting cells. For this purpose, we analyzed in the same experiment monocytes isolated from Fresh Blood and from Overnight Blood Buffy Coats. We assessed lipid antigen presentation by CD1d to invariant Natural Killer T (iNKT) cells. CD1d is a non-polymorphic MHC class I-like protein, which facilitates the study of antigen presentation among allogeneic samples. The results show that monocytes from Fresh Blood Buffy Coats have a better capacity to present antigens by CD1d, and consequently to activate iNKT cells, when compared to monocytes from Overnight Blood Buffy Coats. The differences observed were not explained by disparities in monocyte viability, CD1d expression, or basal activation state (monocyte expression of CD40 and CD80). Buffy Coats are a valid source of blood cells available daily. Hence, the type of protocol for Buffy Coat processing should be carefully considered in day-to-day research, since it may lead to different outcomes.
Characterization of the Lower Airways and Oral Microbiota in Healthy Young Persons in the Community
Authors: Leitao Filho Fernando Sergio , Monica Peters Carli , Sheel Andrew William , Yang Julia , Nislow Corey , Lam Stephen , Leung Janice M. , Sin Don D.
Published: March 2023

Lower airway dysbiosis contributes to disease pathogenesis in respiratory diseases. However, little is known regarding the microbiota of lower airways or the oral cavity of healthy young persons. To address this gap, 25 healthy persons (24.3 ± 3.3 years; 52% females; no current smokers) underwent bronchoscopy during which bronchial brushing (BB) and bronchoalveolar lavage (BAL) fluid were collected. Prior to the procedure, an oral wash (OW) sample was also obtained. Microbiome analyses (16S rRNA locus) were performed (alpha- and beta-diversity, taxa annotations, and predicted functional metagenomic profiles) according to the airway compartment (BB, BAL, and OW). The greatest microbial richness was observed in OW and the lowest in BB (p < 0.001). Microbial communities differed significantly across compartments (p < 0.001), especially between BB and OW. Taxa analyses showed a significantly higher abundance of Firmicutes (BB: 32.7%; BAL: 31.4%) compared to OW (20.9%) (p < 0.001). Conversely, Proteobacteria predominated in OW (27.9%) as opposed to BB (7.0%) and BAL (12.5%) (p < 0.001), mostly due to a greater abundance of the bacteria in the Haemophilus genus in the OW (p < 0.001). The lower airway microbiota (BB and BAL) is significantly different from the OW microbiota in healthy young persons with respect to microbial diversity, taxa profiles, and predicted function.
An Integrative Model of In-Hospital and Out-of-Hospital Nursing Care for Non-Suicidal Self-Injury: A Narrative Review
Authors: Zhang Ruili , Lai Jianbo , Wang Huafen
Published: March 2023

Background: Non-suicidal self-injury (NSSI) refers to the intentional and repeated physical trauma of an individual without explicit suicidal intent, which has negative effects on the physical and mental well-being of an individual, especially for adolescents. Timely and accessible nursing care may play an important role in the survival and rehabilitation process of NSSI. Methods: In this review, we systematically discuss the nursing care of NSSI behavior and provide recommendations based on an integrated nursing model for NSSI management. Results: As reported in previous studies, a variety of factors can contribute to NSSI behavior, such as personality traits, current psychological status, history of mental illnesses, as well as family and social factors. In-hospital care is the most necessary and effective care during acute episodes of NSSI behavior. To effectively manage inpatients with NSSI behaviors, nurses should first understand the motivation of NSSI, and provide comprehensive and multi-level care through nurse-patient communication, individualized psychological care, and multidisciplinary cooperation with other professionals. While the purpose of out-of-hospital nursing is to reduce the frequency of NSSI behaviors by screening high-risk individuals, providing psychological support, promoting health education, and improving self-awareness. Conclusions: An integrative model of in-hospital and out-of-hospital nursing care can help improve the clinical management and long-term prognosis of patients with NSSI and minimize the risk of suicidal ideation or suicidal behavior.
Closed-Loop tACS Delivered during Slow-Wave Sleep Reduces Retroactive Interference on a Paired-Associates Learning Task
Authors: Jones Aaron P. , Bryant Natalie B. , Robert Bradley M. , Mullins Teagan S. , Trumbo Michael C. S. , Ketz Nicholas A. , Howard Michael D. , Pilly Praveen K. , Clark Vincent P.
Published: March 2023

Previous studies have found a benefit of closed-loop transcranial alternating current stimulation (CL-tACS) matched to ongoing slow-wave oscillations (SWO) during sleep on memory consolidation for words in a paired associates task (PAT). Here, we examined the effects of CL-tACS in a retroactive interference PAT (ri-PAT) paradigm, where additional stimuli were presented to increase interference and reduce memory performance. Thirty-one participants were tested on a PAT before sleep, and CL-tACS was applied over the right and left DLPFC (F3 and F4) vs. mastoids for five cycles after detection of the onset of each discrete event of SWO during sleep. Participants were awoken the following morning, learned a new PAT list, and then were tested on the original list. There was a significant effect of stimulation condition (p = 0.04297; Cohen’s d = 0.768), where verum stimulation resulted in reduced retroactive interference compared with sham and a significant interaction of encoding strength and stimulation condition (p = 0.03591). Planned simple effects testing within levels of encoding revealed a significant effect of stimulation only for low-encoders (p = 0.0066; Cohen’s d = 1.075) but not high-encoders. We demonstrate here for the first time that CL-tACS during sleep can enhance the protective benefits on retroactive interference in participants who have lower encoding aptitude.
Relationship between Perceived Pain Interference and Poor Psychological Wellbeing among United States Adults
Authors: Axon David R. , Kim Ann
Published: March 2023

The goal of this observational project was to investigate the association among perceived pain interference and poor psychological wellbeing in United States adults. Adults over 18 years of age in the 2019 Medical Expenditure Panel Survey were eligible for inclusion if they were alive for the calendar year and had data available for their pain status. Hierarchical logistical regression examined statistically significant associations among perceived pain interference and poor psychological wellbeing. Results showed that greater levels of perceived pain interference were significantly related with larger odds of reporting poor psychological wellbeing. Additionally, several other variables were related with larger or lower odds of reporting poor psychological wellbeing. These findings provide insight into the effect of perceived pain interference and other variables with poor psychological wellbeing, which may help recuperate the psychological wellbeing of US adults with pain.
Effects of Resistance Exercise on Cognitive Performance and Depressive Symptoms in Community-Dwelling Older Chinese Americans: A Pilot Randomized Controlled Trial
Authors: Chen Mei-Lan , Wu Ying-Jung , Lee Mi-Jung , Hsieh Sung-Lin , Tseng Ing-Jy , Chen Li-Sheng , Gardenhire Douglas S.
Published: March 2023

Previous literature has suggested physical exercise may improve cognitive impairments and mitigate depressive symptoms. However, few studies examined the impact of resistance exercise intervention on cognition and depression in older Chinese Americans. The purpose of this pilot study was to assess the effects of resistance exercise training on cognitive performance and depressive symptoms among community-dwelling older Chinese Americans. The study was a two-arm randomized controlled trial with pre-test/post-test design. Thirty older adults were randomly assigned into the resistance exercise intervention group or the wait-list control group. Participants’ cognitive performance and depressive symptoms were evaluated at baseline (pre-test) and at 12 weeks (post-test). The results showed that there were significant differences between the intervention and control groups on changes in symptoms of depression, global cognitive function, visuospatial/executive functions, attention, language, and orientation. However, there were no significant differences between both groups on changes in naming, abstraction, and delayed recall domains. The findings of this study suggest that resistance exercise training has a positive impact on improving cognitive performance and depressive symptoms in older adults.
Redevelopment Initiatives on Brownfield Sites: An Evaluation Model for the Definition of Sustainable Investments
Authors: Tajani Francesco , Morano Pierluigi , Di Liddo Felicia
Published: March 2023

With reference to brownfield sites redevelopment interventions, an innovative model for the definition of effective and financially sustainable initiatives is proposed and tested. The model borrows the operative logic of the break-even analysis (BEA). It neglects the basic assumption of BEA related to revenue linearity, by considering the real trend of the revenues. In fact, in specific contexts characterized by a real estate over supply—e.g., in small urban centers, or where a new plan includes a relevant increase in new buildings and/or the functional reconversion of existing disused complexes—the BEA hypothesis on revenue linearity could be inconsistent, as prices will tend to become depressed. In the mentioned situations, discount mechanisms on the unit prices could occur. These phenomena determine a reduction in the unit selling price in correspondence of the amount of gross floor area (GFA) increase. Taking into account the current and cogent needs of effective strategies for brownfields renovation, the innovative evaluation model is developed for supporting public and private investors’ decision processes. It could represent a valid reference in the preliminary phases of decision-making processes for public and private subjects, able to ensure the break-even point of the initiative balance sheet is reached.
Experimental and Numerical Analyses on the Fire Resistance of Timber–Concrete Composite Boards Using an Innovative Form of Partial Protection
Authors: Zhou Hao , Lu Weidong , Lu Binhui , Wang Lu , Bao Yingwei , Zhang Jun , Chen Zhentao
Published: March 2023

Compared with traditional timber boards, timber–concrete composite (TCC) boards demonstrate a higher rigidity and bearing capacity, improved vibration, and better behavior under seismic conditions. However, they become charred when exposed to fire due the combustibility of timber, and the fire safety of this material is considered essential. In this research, 60 min fire exposure tests and residual load-carrying capacity tests following fire exposure were carried out on three full-scale composite boards, two of which were covered with an innovative form of gypsum board protection. The effect of the innovative protection on the temperature field and fire resistance of the TCC boards was studied in detail. The test results indicate that the fire resistance of the TCC boards was effectively improved by using the innovative protection. If the coverage ratio is identical, a wider single gypsum board can demonstrate a slight increase in residual carrying capacity. Finite element models of TCC boards were established to investigate the temperature field during fire exposure and the residual load-carrying capacity of the TCC boards after fire exposure, demonstrating high applicability and accuracy. The conclusions in this paper can provide reference for fire design in engineering.
The Effect of Using Tall Windows in Buildings on the Thermal Load of the Building and Its Cost Analysis: A Comparative Case Study for Antalya and Erzurum
Authors: Efe Sukran
Published: March 2023

The 150 cm tall windows that used as the standard in residential buildings are now being replaced by 220 cm tall windows, which are rapidly becoming widespread. This study examined different window types according to both the type of glass used as well as the type of fill material used in double-glazed windows in order to show the effect of window selection on the energy consumption of the building. A comparison between Antalya and Erzurum was also made in the study. The study was carried out on a building model with seven floors and twenty-four apartments, each of which has five units. The window heights in each unit were assumed to be 150 cm and were defined as Type 0. Five additional building types were defined by increasing the window heights in each unit, respectively. Thermal load values were calculated for all six building types and compared with each other. As a result, a 46.7% increase in the window area causes a maximum heat loss of 9.6% in Erzurum and a maximum heat gain of 45.1% in Antalya. These values bring about an increase in the energy consumption by an average of USD 1465.32 per year in Erzurum and an average of USD 581.08 per year in Antalya.
Exploring the Project-Based Collaborative Networks between Owners and Contractors in the Construction Industry: Empirical Study in China
Authors: Wang Fangliang , Cheng Min , Cheng Xiaotong
Published: March 2023

In the project-based construction industry, organizations build collaborative relationships through specific projects. The owners and contractors who are the key project stakeholders have gradually formed a complex project-based industry-level collaborative network in many different projects, closely related to knowledge exchange and industry development. Based on the data set of the National Quality Engineering Award (NQEA) projects in China from 2013 to 2021, we empirically analyze the characteristics and evolution of project-based collaborative networks between owners and contractors in the construction industry by using social network analysis (SNA) and network motif analysis (NMA) method. The results show that (1) the owner–contractor collaborative network exhibits small-world network characteristics. The island effect caused by small groups in the network makes the overall connectivity of the network low. During the study period, the collaborative network became more compact. (2) State-owned construction companies, such as China Construction Third Engineering Bureau Corporation Limited, China Construction Eighth Engineering Bureau Corporation Limited, and China Construction Second Engineering Bureau Corporation Limited, with high degree centrality and betweenness centrality, are the core companies in the collaborative network. In China, state-owned construction enterprises are favored by owners and have established collaborative relationships with many owners and contractors. (3) There are two local collaborative patterns in the collaborative network: motif and anti-motif. Motifs include some triangle-based tight collaborative patterns, while anti-motifs involve some loose binary collaborative patterns. The results help understand the structure and evolution of the industry-level collaborative relationship network between owners and contractors and can provide references for owners and contractors to develop relationship cultivation strategies more effectively.
Optical Genome Mapping for Cytogenetic Diagnostics in AML
Authors: Nilius-Eliliwi Verena , Gerding Wanda M. , Schroers Roland , Nguyen Huu Phuc , Vangala Deepak B.
Published: March 2023

The classification and risk stratification of acute myeloid leukemia (AML) is based on reliable genetic diagnostics. A broad and expanding variety of relevant aberrations are structural variants beyond single-nucleotide variants. Optical Genome Mapping is an unbiased, genome-wide, amplification-free method for the detection of structural variants. In this review, the current knowledge of Optical Genome Mapping (OGM) with regard to diagnostics in hematological malignancies in general, and AML in specific, is summarized. Furthermore, this review focuses on the ability of OGM to expand the use of cytogenetic diagnostics in AML and perhaps even replace older techniques such as chromosomal-banding analysis, fluorescence in situ hybridization, or copy number variation microarrays. Finally, OGM is compared to amplification-based techniques and a brief outlook for future directions is given.
Factors Associated with the Decision to Decline Chemotherapy in Metastatic Non-Small Cell Lung Cancer
Authors: Jabbal Iktej Singh , Sabbagh Saad , Itani Mira , Dominguez Barbara , Mohanna Mohamed , Henry Valencia , Liang Hong , Saravia Diana , George Tiffany , Nahleh Zeina , Alley Evan , Arteta-Bulos Rafael
Published: March 2023

(1) Background: Disparities in cancer treatment and outcomes have long been well-documented in the medical literature. With the eruption of advances in new treatment modalities, the long-existing disparities are now being further uncovered and brought to the attention of the medical community. While social health determinants have previously been linked to treatment disparities in lung cancer, we analyzed data from the National Cancer Database to explore sociodemographic and geographic factors related to accepting or declining physician-recommended chemotherapy. Patients diagnosed with metastatic lung cancer between 2004 and 2016 who declined chemotherapy recommended by their physicians were included in this study. Multivariate logistic regression analysis was performed. Cox Regression and Kaplan-Meier analyses were performed to look for survival characteristics. (2) Results: 316,826 patients with Stage IV lung cancer were identified. Factors related to a higher rate of refusal by patients included older age > 70, female sex, low income, lack of insurance coverage, residency in the New England region, and higher comorbidity. Patients living in areas with lower education were less likely to decline chemotherapy. (3) Conclusion: Further understanding of the factors impacting treatment decisions would be essential to improve the efficacy of care delivery in patients with cancer and reduce reversible causes of disparity.
Phase 1 Study to Evaluate the Safety of Reducing the Prophylactic Dose of Dexamethasone around Docetaxel Infusion in Patients with Prostate and Breast Cancer
Authors: Lugtenberg Rieneke T. , de Groot Stefanie , Houtsma Danny , Dezentjé Vincent O. , Vulink Annelie J. E. , Fischer Maarten J. , Portielje Johanneke E. A. , van der Hoeven Jacobus J. M. , Gelderblom Hans , Pijl Hanno , Kroep Judith R.
Published: March 2023

Background: There is little evidence that supports the registered high dose of dexamethasone used around docetaxel. However, this high dose is associated with considerable side effects. This study evaluates the feasibility of reducing the prophylactic oral dosage of dexamethasone around docetaxel infusion. Patients and methods: Eligible patients had a histologically confirmed diagnosis of prostate or breast cancer and had received at least three cycles of docetaxel as monotherapy or combination therapy. Prophylactic dexamethasone around docetaxel infusion was administered in a de-escalating order per cohort of patients. Primary endpoint was the occurrence of grade III/IV fluid retention and hypersensitivity reactions (HSRs). Results: Of the 46 enrolled patients, 39 were evaluable (prostate cancer (n = 25), breast cancer (n = 14). In patients with prostate cancer, the dosage of dexamethasone was reduced to a single dose of 4 mg; in patients with breast cancer, the dosage was reduced to a 3-day schedule of 4 mg–8 mg–4 mg once daily, after which no further reduction has been tested. None of the 39 patients developed grade III/IV fluid retention or HSR. One patient (2.6%) had a grade 1 HSR, and there were six patients (15.4%) with grade I or II edema. There were no differences in quality of life (QoL) between cohorts. Conclusions: It seems that the prophylactic dose of dexamethasone around docetaxel infusion can be safely reduced with respect to the occurrence of grade III/IV HSRs or the fluid retention syndrome.
Improved Light Hydrocarbon, Furans, and BTEX Production from the Catalytic Assisted Pyrolysis of Agave salmiana Bagasse over Silica Mesoporous Catalysts
Authors: Santiago-Martínez Leoncio , González-Falcón César Irán , Reyes-Hernández Jaime , Handy Brent E. , Cárdenas-Galindo María-Guadalupe
Published: March 2023

The pyrolysis of the biomass Agave salmiana bagasse (10 K/min, ambient to 700 °C) was investigated in the absence and presence of Aerosil and MCM-41 catalysts. MCM-41 was synthetized using a typical hydrothermal method and characterized with XRD, SAXS, SEM, TEM, and nitrogen physisorption to confirm the presence of unidimensional 3.4 nm diameter pores. Pyrolysis products were monitored online with mass spectrometry (MS), analyzing the production of 29 different compounds, clustered in several groups, namely, olefins (ethene, 2-butene, 1,3-butadiene), oxygenated compounds (methanol, 2-methylbutanol, acetic acid), furan derivatives (furan, furfural, 2-methylfurane), and aromatic compounds (BTEX). Complete decomposition of the cellulose and hemicellulose content of the biomass was observed at temperatures below 400 °C. Lignin decomposition was completed by 550 °C. Catalyst-assisted pyrolysis showed reduced acetic acid and methanol formation with Aerosil and MCM-41. The use of Aerosil does not affect the overall production of olefins, yet increases benzene yield, while reducing the production of phenol, furan, and furan derivatives. With MCM-41, there is increased production of olefins, furan, furan derivatives, cyclohexanone and BTEX, yet phenol production is decreased. At temperatures below 400 °C, the product formation pattern is comparable to non-catalytic pyrolysis.
New Insights into the Surfactant-Assisted Liquid-Phase Exfoliation of Bi2S3 for Electrocatalytic Applications
Authors: Wang Mengjiao , Crisci Matteo , Pavan Matilde , Liu Zheming , Gallego Jaime , Gatti Teresa
Published: March 2023

During water electrolysis, adding an electrocatalyst for the hydrogen evolution reaction (HER) is necessary to reduce the activation barrier and thus enhance the reaction rate. Metal chalcogenide-based 2D nanomaterials have been studied as an alternative to noble metal electrocatalysts because of their interesting electrocatalytic properties and low costs of production. However, the difficulty in improving the catalytic efficiency and industrializing the synthetic methods have become a problem in the potential application of these species in electrocatalysis. Liquid-phase exfoliation (LPE) is a low-cost and scalable technique for lab- and industrial-scale synthesis of 2D-material colloidal inks. In this work, we present, to the best of our knowledge, for the first time a systematic study on the surfactant-assisted LPE of bulk Bi2S3 crystalline powder to produce nanosheets (NSs). Different dispersing agents and LPE conditions have been tested in order to obtain colloidal low-dimensional Bi2S3 NSs in H2O at optimized concentrations. Eventually, colloidally stable layered nano-sized Bi2S3 suspensions can be produced with yields of up to ~12.5%. The thus obtained low-dimensional Bi2S3 is proven to be more active for HER than the bulk starting material, showing an overpotential of only 235 mV and an optimized Tafel slope of 125 mV/dec. Our results provide a facile top-down method to produce nano-sized Bi2S3 through a green approach and demonstrate that this material can have a good potential as electrocatalyst for HER.
Recent Advances in Greener Asymmetric Organocatalysis Using Bio-Based Solvents
Authors: Martelli Lorena S. R. , Machado Ingrid V. , dos Santos Jhonathan R. N. , Corrêa Arlene G.
Published: March 2023

Efficient synthetic methods that avoid the extensive use of hazardous reagents and solvents, as well as harsh reaction conditions, have become paramount in the field of organic synthesis. Organocatalysis is notably one of the best tools in building chemical bonds between carbons and carbon-heteroatoms; however, most examples still employ toxic volatile organic solvents. Although a portfolio of greener solvents is now commercially available, only ethyl alcohol, ethyl acetate, 2-methyltetrahydrofuran, supercritical carbon dioxide, ethyl lactate, and diethyl carbonate have been explored with chiral organocatalysts. In this review, the application of these bio-based solvents in asymmetric organocatalytic methods reported in the last decade is discussed, highlighting the proposed mechanism pathway for the transformations.
Lignin Valorization: Production of High Value-Added Compounds by Engineered Microorganisms
Authors: Rosini Elena , Molinari Filippo , Miani Davide , Pollegioni Loredano
Published: March 2023

Lignin is the second most abundant polymer in nature, which is also widely generated during biomass fractionation in lignocellulose biorefineries. At present, most of technical lignin is simply burnt for energy supply although it represents the richest natural source of aromatics, and thus it is a promising feedstock for generation of value-added compounds. Lignin is heterogeneous in composition and recalcitrant to degradation, with this substantially hampering its use. Notably, microbes have evolved particular enzymes and specialized metabolic pathways to degrade this polymer and metabolize its various aromatic components. In recent years, novel pathways have been designed allowing to establish engineered microbial cell factories able to efficiently funnel the lignin degradation products into few metabolic intermediates, representing suitable starting points for the synthesis of a variety of valuable molecules. This review focuses on recent success cases (at the laboratory/pilot scale) based on systems metabolic engineering studies aimed at generating value-added and specialty chemicals, with much emphasis on the production of cis,cis-muconic acid, a building block of recognized industrial value for the synthesis of plastic materials. The upgrade of this global waste stream promises a sustainable product portfolio, which will become an industrial reality when economic issues related to process scale up will be tackled.