bluuebunny/crossref_metadata_2025 huggingface dataset:
Diversity and Distribution of Resistance Markers in Pseudomonas aeruginosa International High-Risk Clones
Authors: Kocsis Béla , Gulyás Dániel , Szabó Dóra
Published: February 2021

Pseudomonas aeruginosa high-risk clones are disseminated worldwide and they are common causative agents of hospital-acquired infections. In this review, we will summarize available data of high-risk P. aeruginosa clones from confirmed outbreaks and based on whole-genome sequence data. Common feature of high-risk clones is the production of beta-lactamases and among metallo-beta-lactamases NDM, VIM and IMP types are widely disseminated in different sequence types (STs), by contrast FIM type has been reported in ST235 in Italy, whereas GIM type in ST111 in Germany. In the case of ST277, it is most frequently detected in Brazil and it carries a resistome linked to blaSPM. Colistin resistance develops among P. aeruginosa clones in a lesser extent compared to other resistance mechanisms, as ST235 strains remain mainly susceptible to colistin however, some reports described mcr positive P. aeurigonsa ST235. Transferable quinolone resistance determinants are detected in P. aeruginosa high-risk clones and aac(6′)-Ib-cr variant is the most frequently reported as this determinant is incorporated in integrons. Additionally, qnrVC1 was recently detected in ST773 in Hungary and in ST175 in Spain. Continuous monitoring and surveillance programs are mandatory to track high-risk clones and to analyze emergence of novel clones as well as novel resistance determinants.
How the Soil Microbial Communities and Activities Respond to Long-Term Heavy Metal Contamination in Electroplating Contaminated Site
Authors: Gong Wen-Jing , Niu Zi-Fan , Wang Xing-Run , Zhao He-Ping
Published: February 2021

The effects of long-term heavy metal contamination on the soil biological processes and soil microbial communities were investigated in a typical electroplating site in Zhangjiakou, China. It was found that the soil of the electroplating plant at Zhangjiakou were heavily polluted by Cr, Cr (VI), Ni, Cu, and Zn, with concentrations ranged from 112.8 to 9727.2, 0 to 1083.3, 15.6 to 58.4, 10.8 to 510.0 and 69.6 to 631.6 mg/kg, respectively. Soil urease and phosphatase activities were significantly inhibited by the heavy metal contamination, while the microbial biomass carbon content and the bacterial community richness were much lower compared to noncontaminated samples, suggesting that the long-term heavy metal contamination had a severe negative effect on soil microorganisms. Differently, soil dehydrogenase was promoted in the presence of Chromate compared to noncontaminated samples. This might be due to the enrichment of Sphingomonadaceae, which have been proven to be able to secrete dehydrogenase. The high-throughput sequencing of the 16S rRNA gene documented that Proteobacteria, Actinobacteria, and Chloroflexi were the dominant bacterial phyla in the contaminated soil. The Spearman correlation analysis showed the Methylobacillus, Muribaculaceae, and Sphingomonadaceae were able to tolerate high concentrations of Cr, Cr (VI), Cu, and Zn, indicating their potential in soil remediation.
Metallic Nanoparticles—Friends or Foes in the Battle against Antibiotic-Resistant Bacteria?
Authors: Amaro Francisco , Morón Álvaro , Díaz Silvia , Martín-González Ana , Gutiérrez Juan Carlos
Published: February 2021

The rapid spread of antibiotic resistances among bacteria demands novel strategies for infection control, and metallic nanoparticles appear as promising tools because of their unique size and tunable properties that allow their antibacterial effects to be maximized. Furthermore, their diverse mechanisms of action towards multiple cell components have suggested that bacteria could not easily develop resistance against nanoparticles. However, research published over the last decade has proven that bacteria can indeed evolve stable resistance mechanisms upon continuous exposure to metallic nanoparticles. In this review, we summarize the currently known individual and collective strategies employed by bacteria to cope with metallic nanoparticles. Importantly, we also discuss the adverse side effects that bacterial exposure to nanoparticles may have on antibiotic resistance dissemination and that might constitute a challenge for the implementation of nanoparticles as antibacterial agents. Overall, studies discussed in this review point out that careful management of these very promising antimicrobials is necessary to preserve their efficacy for infection control.
Changes of the Proteome and Acetylome during Transition into the Stationary Phase in the Organohalide-Respiring Dehalococcoides mccartyi Strain CBDB1
Authors: Greiner-Haas Franziska , Bergen Martin von , Sawers Gary , Lechner Ute , Türkowsky Dominique
Published: February 2021

The strictly anaerobic bactGIerium Dehalococcoides mccartyi obligatorily depends on organohalide respiration for energy conservation and growth. The bacterium also plays an important role in bioremediation. Since there is no guarantee of a continuous supply of halogenated substrates in its natural environment, the question arises of how D. mccartyi maintains the synthesis and activity of dehalogenating enzymes under these conditions. Acetylation is a means by which energy-restricted microorganisms can modulate and maintain protein levels and their functionality. Here, we analyzed the proteome and Nε-lysine acetylome of D. mccartyi strain CBDB1 during growth with 1,2,3-trichlorobenzene as an electron acceptor. The high abundance of the membrane-localized organohalide respiration complex, consisting of the reductive dehalogenases CbrA and CbdbA80, the uptake hydrogenase HupLS, and the organohalide respiration-associated molybdoenzyme OmeA, was shown throughout growth. In addition, the number of acetylated proteins increased from 5% to 11% during the transition from the exponential to the stationary phase. Acetylation of the key proteins of central acetate metabolism and of CbrA, CbdbA80, and TatA, a component of the twin-arginine translocation machinery, suggests that acetylation might contribute to maintenance of the organohalide-respiring capacity of the bacterium during the stationary phase, thus providing a means of ensuring membrane protein integrity and a proton gradient.
Garnet-Vesuvianite Equilibrium in Rodingites from Dobšiná (Western Carpathians)
Authors: Butek Juraj , Spišiak Ján , Milovská Stanislava
Published: February 2021

Intensively metasomatized rocks from serpentinized ultramafic tectonic fragments in Dobšiná, Western Carpathians, consist of typical rodingite mineral association: hydrated garnet, vesuvianite, diopside and clinochlore. Electron microprobe analysis (EMPA) and Micro-Raman analyses of the main minerals evidence complex mineralogical evolution and variable mineral chemistry. Garnet solid solution is dominated by grossular-andradite series, which demonstrates a significant degree of hydration, mainly for grossular rich garnet cores. Garnet is locally enriched in TiO2 (up to 13 wt%), possibly indicating a chemical relic of a Ti-oxide mineral. Younger, andradite-richer garnet rims demonstrate a low degree of hydration, suggesting a harder incorporation of an (OH)− anion into its crystal structure. Garnet chemical variations display an ideal negative correlation between Al and (Fe3+ + Ti). The most recent mineral phase is represented by euhedral vesuvianite (± chlorite), which crystallizes at the expense of the garnet solid solution. This reaction shows a well-equilibrated character and indicates a high extent of rodingitization process. Chlorite thermometry models suggest an average temperature of late rodingite (trans) formation of about 265 °C.
A Model Structure for Size-by-Liberation Recoveries in Flotation
Authors: Vallejos Paulina , Yianatos Juan , Vinnett Luis
Published: February 2021

This communication presents a model structure for the flotation recovery of middling particles (10–90% liberation). Fourteen datasets from the literature were studied (galena flotation), which involved different flotation systems and operating conditions. The flotation responses allowed the model flexibility to be evaluated under a range of recovery profiles. The modelling results showed that galena recovery can be characterized by the interaction between a linear function and a concave function (e.g., Gamma model), to account for the liberation and particle size effects, respectively. Liberation also impacts the location and dispersion of the recovery dependence on particle size. The proposed model structure showed there was adequate flexibility with five parameters, leading to adjusted coefficients of determination ranging from 0.863 to 0.998 for the studied datasets. Thus, an alternative approach for modelling the recovery of middling particles is proposed, which represents the liberation and particle size dependence with a few parameters.
Applications of Vibrational Spectroscopy for Analysis of Connective Tissues
Authors: Querido William , Kandel Shital , Pleshko Nancy
Published: February 2021

Advances in vibrational spectroscopy have propelled new insights into the molecular composition and structure of biological tissues. In this review, we discuss common modalities and techniques of vibrational spectroscopy, and present key examples to illustrate how they have been applied to enrich the assessment of connective tissues. In particular, we focus on applications of Fourier transform infrared (FTIR), near infrared (NIR) and Raman spectroscopy to assess cartilage and bone properties. We present strengths and limitations of each approach and discuss how the combination of spectrometers with microscopes (hyperspectral imaging) and fiber optic probes have greatly advanced their biomedical applications. We show how these modalities may be used to evaluate virtually any type of sample (ex vivo, in situ or in vivo) and how “spectral fingerprints” can be interpreted to quantify outcomes related to tissue composition and quality. We highlight the unparalleled advantage of vibrational spectroscopy as a label-free and often nondestructive approach to assess properties of the extracellular matrix (ECM) associated with normal, developing, aging, pathological and treated tissues. We believe this review will assist readers not only in better understanding applications of FTIR, NIR and Raman spectroscopy, but also in implementing these approaches for their own research projects.
Colour Me Blue: The History and the Biotechnological Potential of Pyocyanin
Authors: Gonçalves Thiago , Vasconcelos Ulrich
Published: February 2021

Pyocyanin was the first natural phenazine described. The molecule is synthesized by about 95% of the strains of Pseudomonas aeruginosa. From discovery up to now, pyocyanin has been characterised by a very rich and avant-garde history, which includes its use in antimicrobial therapy, even before the discovery of penicillin opened the era of antibiotic therapy, as well as its use in electric current generation. Exhibiting an exuberant blue colour and being easy to obtain, this pigment is the subject of the present review, aiming to narrate its history as well as to unveil its mechanisms and suggest new horizons for applications in different areas of engineering, biology and biotechnology.
One-Step Synthesis of Magnetic Nanocomposite with Embedded Biologically Active Substance
Authors: Ignatovich Zhanna , Novik Khristina , Abakshonok Anna , Koroleva Elena , Beklemisheva Anna , Panina Larisa , Kaniukov Egor , Anisovich Marina , Shumskaya Alena
Published: February 2021

Magnetic nanocomposites based on hydroxyapatite were prepared by a one-step process using the hydrothermal coprecipitation method to sinter iron oxides (Fe3O4 and γ-Fe2O3). The possibility of expanding the proposed technique for the synthesis of magnetic composite with embedded biologically active substance (BAS) of the 2-arylaminopyrimidine group was shown. The composition, morphology, structural features, and magnetic characteristics of the nanocomposites synthesized with and without BAS were studied. The introduction of BAS into the composite synthesis resulted in minor changes in the structural and physical properties. The specificity of the chemical bonds between BAS and the hydroxyapatite-magnetite core was revealed. The kinetics of the BAS release in a solution simulating the stomach environment was studied. The cytotoxicity of (HAP)FexOy and (HAP)FexOy + BAS composites was studied in vitro using the primary culture of human liver carcinoma cells HepG2. The synthesized magnetic composites with BAS have a high potential for use in the biomedical field, for example, as carriers for magnetically controlled drug delivery and materials for bone tissue engineering.
Phytochemical Screening and Evaluation of Antioxidant Properties and Antimicrobial Activity against Xanthomonas axonopodis of Euphorbia tirucalli Extracts in Binh Thuan Province, Vietnam
Authors: Le Nguyen Thị My , Cuong Dang Xuan , Thinh Pham Van , Minh Truong Ngoc , Manh Tran Dinh , Duong Thuc-Huy , Minh Tran Thi Le , Oanh Vo Thi Thu
Published: February 2021

Euphorbia tirucalli is a medicine plant possessing many bioactive properties. This paper focused on phytochemical screening (alkaloid, flavonoid, saponin, tannin, and anthraquinone), quantification of polyphenol and flavonoids, and activating evaluation of antioxidants and antimicrobial properties against Xanthomonas axonopodis of different extracts from Euphorbia tirucalli grown in Binh Thuan, Vietnam. The best activity fraction was used for purification and determining bioactive ingredients. The results showed that the phytochemical study revealed the presence of alkaloids, flavonoids, tannins, and terpenoids in the ethyl acetate fraction. Saponin and anthraquinone did not present in all extracts. The content of polyphenol and flavonoid of Euphorbia tirucalli stem was in the range of 16.65–106.32 mg EqAG/g and 97.97–450.83 μg QE/g. The ethyl acetate fraction showed higher amounts of polyphenol and flavonoids and antimicrobial activity against X. axonopodis than other fractions. The antioxidant (SC50) activity of Euphorbia tirucalli stem was in the range of 12.91 ± 0.70 and 528.33 ± 25.15 μg/mL. At concentrations of 5.0 and 7.5 mg/mL, the diameter of inhibition of the ethyl acetate fraction was 14.33 ± 0.76 mm and 17.87 ± 0.57 mm, respectively. The MIC (minimum inhibitory concentration) was 0.156 mg/mL. Scopoletin, gallic acid, and piperic acid got MICs corresponding to 78, 312, and 312 μg/mL, respectively. Scopoletin, gallic acid, and piperic acid were found in the ethyl acetate fraction of Euphorbia tirucalli and exhibited the treatment of citrus bacteria canker and plant diseases.
Anti-Malignant Ascites Effect of Total Diterpenoids from Euphorbiae ebracteolatae Radix Is Attributable to Alterations of Aquaporins via Inhibiting PKC Activity in the Kidney
Authors: Zhang Yuanbin , Liu Dongfang , Xue Fan , Yu Hongli , Wu Hao , Cui Xiaobing , Zhang Xingde , Wang Hepeng
Published: February 2021

This study evaluated the anti-ascites effect of total diterpenoids extracted from Euphorbiae ebracteolatae Radix (TDEE) on malignant ascitic mice and elucidated its underlying mechanism. TDEE was extracted by dichloromethane and subjected to column chromatography. The purity of six diterpenoids isolated from TDEE was determined to be 77.18% by HPLC. TDEE (3 and 0.6 g raw herbs/kg, p.o.) reduced ascites and increased urine output. Meanwhile, analysis of tumor cell viability, cycle and apoptosis indicated that TDEE had no antitumor activity. In addition, the expression levels of aquaporins (AQPs) and the membrane translocation levels of protein kinase C (PKC) α and PKCβ in kidney and cells were measured. TDEE reduced the levels of AQP1–4, and inhibited PKCβ expression in membrane fraction. Four main diterpenoids, except compound 2, reduced AQP1 level in human kidney-2 cells. Compounds 4 and 5 inhibited AQP2–4 expression in murine inner medullary collecting duct cells. The diterpenoid-induced inhibition of AQP1–4 expression was blocked by phorbol-12-myristate-13-acetate (PMA; agonist of PKC). The diterpenoids from TDEE are the main anti-ascites components. The anti-ascites effect of diterpenoids may be associated with alterations in AQPs in the kidneys to promote diuresis. The inhibition of AQP1–4 expression by TDEE is related to the inhibition of PKCβ activation.
Strategies to Target ADAM17 in Disease: From Its Discovery to the iRhom Revolution
Authors: Calligaris Matteo , Cuffaro Doretta , Bonelli Simone , Spanò Donatella Pia , Rossello Armando , Nuti Elisa , Scilabra Simone Dario
Published: February 2021

For decades, disintegrin and metalloproteinase 17 (ADAM17) has been the object of deep investigation. Since its discovery as the tumor necrosis factor convertase, it has been considered a major drug target, especially in the context of inflammatory diseases and cancer. Nevertheless, the development of drugs targeting ADAM17 has been harder than expected. This has generally been due to its multifunctionality, with over 80 different transmembrane proteins other than tumor necrosis factor α (TNF) being released by ADAM17, and its structural similarity to other metalloproteinases. This review provides an overview of the different roles of ADAM17 in disease and the effects of its ablation in a number of in vivo models of pathological conditions. Furthermore, here, we comprehensively encompass the approaches that have been developed to accomplish ADAM17 selective inhibition, from the newest non-zinc-binding ADAM17 synthetic inhibitors to the exploitation of iRhom2 to specifically target ADAM17 in immune cells.
Density Functional Theory Study of Optical and Electronic Properties of (TiO2)n=5,8,68 Clusters for Application in Solar Cells
Authors: Elegbeleye Ife Fortunate , Maluta Nnditshedzeni Eric , Maphanga Rapela Regina
Published: February 2021

A range of solution-processed organic and hybrid organic−inorganic solar cells, such as dye-sensitized and bulk heterojunction organic solar cells have been intensely developed recently. TiO2 is widely employed as electron transporting material in nanostructured TiO2 perovskite-sensitized solar cells and semiconductor in dye-sensitized solar cells. Understanding the optical and electronic mechanisms that govern charge separation, transport and recombination in these devices will enhance their current conversion efficiencies under illumination to sunlight. In this work, density functional theory with Perdew-Burke Ernzerhof (PBE) functional approach was used to explore the optical and electronic properties of three modeled TiO2 brookite clusters, (TiO2)n=5,8,68. The simulated optical absorption spectra for (TiO2)5 and (TiO2)8 clusters show excitation around 200–400 nm, with (TiO2)8 cluster showing higher absorbance than the corresponding (TiO2)5 cluster. The density of states and the projected density of states of the clusters were computed using Grid-base Projector Augmented Wave (GPAW) and PBE exchange correlation functional in a bid to further understand their electronic structure. The density of states spectra reveal surface valence and conduction bands separated by a band gap of 1.10, 2.31, and 1.37 eV for (TiO2)5, (TiO2)8, and (TiO2)68 clusters, respectively. Adsorption of croconate dyes onto the cluster shifted the absorption peaks to higher wavelengths.
Effect of Side Chain Substituent Volume on Thermoelectric Properties of IDT-Based Conjugated Polymers
Authors: Xie De-Xun , Liu Tong-Chao , Xiao Jing , Fang Jing-Kun , Pan Cheng-Jun , Shao Guang
Published: February 2021

A p-type thermoelectric conjugated polymer based on indacenodithiophene and benzothiadiazole is designed and synthesized by replacing normal aliphatic side chains (P1) with conjugated aromatic benzene substituents (P2). The introduced bulky substituent on P2 is detrimental to form the intensified packing of polymers, therefore, it hinders the efficient transporting of the charge carriers, eventually resulting in a lower conductivity compared to that of the polymers bearing aliphatic side chains (P1). These results reveal that the modification of side chains on conjugated polymers is crucial to rationally designed thermoelectric polymers with high performance.
Electron Paramagnetic Resonance Gives Evidence for the Presence of Type 1 Gonadotropin-Releasing Hormone Receptor (GnRH-R) in Subdomains of Lipid Rafts
Authors: Koklič Tilen , Hrovat Alenka , Guixà-González Ramon , Rodríguez-Espigares Ismael , Navio Damaris , Frangež Robert , Uršič Matjaž , Kubale Valentina , Plemenitaš Ana , Selent Jana , Šentjurc Marjeta , Vrecl Milka
Published: February 2021

This study investigated the effect of type 1 gonadotropin releasing hormone receptor (GnRH-R) localization within lipid rafts on the properties of plasma membrane (PM) nanodomain structure. Confocal microscopy revealed colocalization of PM-localized GnRH-R with GM1-enriched raft-like PM subdomains. Electron paramagnetic resonance spectroscopy (EPR) of a membrane-partitioned spin probe was then used to study PM fluidity of immortalized pituitary gonadotrope cell line αT3-1 and HEK-293 cells stably expressing GnRH-R and compared it with their corresponding controls (αT4 and HEK-293 cells). Computer-assisted interpretation of EPR spectra revealed three modes of spin probe movement reflecting the properties of three types of PM nanodomains. Domains with an intermediate order parameter (domain 2) were the most affected by the presence of the GnRH-Rs, which increased PM ordering (order parameter (S)) and rotational mobility of PM lipids (decreased rotational correlation time (τc)). Depletion of cholesterol by methyl-β-cyclodextrin (methyl-β-CD) inhibited agonist-induced GnRH-R internalization and intracellular Ca2+ activity and resulted in an overall reduction in PM order; an observation further supported by molecular dynamics (MD) simulations of model membrane systems. This study provides evidence that GnRH-R PM localization may be related to a subdomain of lipid rafts that has lower PM ordering, suggesting lateral heterogeneity within lipid raft domains.
Synthesis of Xanthones, Thioxanthones and Acridones by a Metal-Free Photocatalytic Oxidation Using Visible Light and Molecular Oxygen
Authors: Torregrosa-Chinillach Alejandro , Chinchilla Rafael
Published: February 2021

9H-Xanthenes, 9H-thioxanthenes and 9,10-dihydroacridines can be easily oxidized to the corresponding xanthones, thioxanthones and acridones, respectively, by a simple photo-oxidation procedure carried out using molecular oxygen as oxidant under the irradiation of visible blue light and in the presence of riboflavin tetraacetate as a metal-free photocatalyst. The obtained yields are high or quantitative.
Removal of Cu(II) Contamination from Aqueous Solution by Ethylenediamine@β-Zeolite Composite
Authors: Liu Peng , Ruan Hui , Li Tiantian , Chen Jiaqi , Ma Fuqiu , Pan Duoqiang , Wu Wangsuo
Published: February 2021

The low cost β-zeolite and ethylenediamine modified β-zeolite (EDA@β-zeolite) were prepared by self-assembly method and used for Cu(II) removal from contaminated aqueous solution. Removal ability of β-zeolite toward Cu(II) was greatly improved after ethylenediamine (EDA) modification, the removal performance was greatly affected by environmental conditions. XPS results illustrated that the amide group played important role in the removal process by forming complexes with Cu(II). The EDA@β-zeolite showed desirable recycling ability. The finding herein suggested that the proposed composite is a promising and suitable candidate for the removal of Cu(II) from contaminated natural wastewater and aquifer.
Towards Control of the Size, Composition and Surface Area of NiO Nanostructures by Sn Doping
Authors: Taeño María , Maestre David , Ramírez-Castellanos Julio , Li Shaohui , Lee Pooi See , Cremades Ana
Published: February 2021

Achieving nanostructures with high surface area is one of the most challenging tasks as this metric usually plays a key role in technological applications, such as energy storage, gas sensing or photocatalysis, fields in which NiO is gaining increasing attention recently. Furthermore, the advent of modern NiO-based devices can take advantage of a deeper knowledge of the doping process in NiO, and the fabrication of p-n heterojunctions. By controlling experimental conditions such as dopant concentration, reaction time, temperature or pH, NiO morphology and doping mechanisms can be modulated. In this work, undoped and Sn doped nanoparticles and NiO/SnO2 nanostructures with high surface areas were obtained as a result of Sn incorporation. We demonstrate that Sn incorporation leads to the formation of nanosticks morphology, not previously observed for undoped NiO, promoting p-n heterostructures. Consequently, a surface area value around 340 m2/g was obtained for NiO nanoparticles with 4.7 at.% of Sn, which is nearly nine times higher than that of undoped NiO. The presence of Sn with different oxidation states and variable Ni3+/Ni2+ ratio as a function of the Sn content were also verified by XPS, suggesting a combination of two charge compensation mechanisms (electronic and ionic) for the substitution of Ni2+ by Sn4+. These results make Sn doped NiO nanostructures a potential candidate for a high number of technological applications, in which implementations can be achieved in the form of NiO–SnO2 p-n heterostructures.
Nanostructure of Porous Si and Anodic SiO2 Surface Passivation for Improved Efficiency Porous Si Solar Cells
Authors: Sundarapura Panus , Zhang Xiao-Mei , Yogai Ryoji , Murakami Kazuki , Fave Alain , Ihara Manabu
Published: February 2021

The photovoltaic effect in the anodic formation of silicon dioxide (SiO2) on porous silicon (PS) surfaces was investigated toward developing a potential passivation technique to achieve high efficiency nanostructured Si solar cells. The PS layers were prepared by electrochemical anodization in hydrofluoric acid (HF) containing electrolyte. An anodic SiO2 layer was formed on the PS surface via a bottom-up anodization mechanism in HCl/H2O solution at room temperature. The thickness of the oxide layer for surface passivation was precisely controlled by adjusting the anodizing current density and the passivation time, for optimal oxidation on the PS layer while maintaining its original nanostructure. HRTEM characterization of the microstructure of the PS layer confirms an atomic lattice matching at the PS/Si interface. The dependence of photovoltaic performance, series resistance, and shunt resistance on passivation time was examined. Due to sufficient passivation on the PS surface, a sample with anodization duration of 30 s achieved the best conversion efficiency of 10.7%. The external quantum efficiency (EQE) and internal quantum efficiency (IQE) indicate a significant decrease in reflectivity due to the PS anti-reflection property and indicate superior performance due to SiO2 surface passivation. In conclusion, the surface of PS solar cells could be successfully passivated by electrochemical anodization.
Curcumin Nanoformulations with Metal Oxide Nanomaterials for Biomedical Applications
Authors: Beyene Anteneh Marelign , Moniruzzaman Mohammad , Karthikeyan Adhimoolam , Min Taesun
Published: February 2021

In the past few decades, curcumin, a natural polyphenolic phytochemical, has been studied for treating a wide variety of diseases. It has shown promising results as a potential curative agent for a variety of diseases. However, its inherent limitations, such as poor aqueous solubility, poor absorbability, fast metabolic rate, and quick elimination from the body, have limited its application beyond preclinical studies. A huge number of studies have been made to address the issues of curcumin and to maximally utilize its potentials. Many review articles have tried to assess and summarize different nanocarriers, especially organic nanocarriers, for nanoformulations with curcumin. Nevertheless, few exclusive reviews on the progress in nanoformulation of curcumin with inorganic nanomaterials have been made. In this review, we present an exclusive summary of the progress in nanoformulation of curcumin with metal oxide nanoparticles. The beneficial feature of the metal oxide nanoparticles used in the curcumin nanoformulation, the different approaches followed in formulating curcumin with the metal oxides, and the corresponding results, protective effect of curcumin from different metal oxide caused toxicities, and concluding remarks are presented in the review.
Enhanced UV Photoresponsivity of ZnO Nanorods Decorated with Ag2S/ZnS Nanoparticles by Successive Ionic Layer Adsorption and Reaction Method
Authors: Jin Yimin , Jiao Shujie , Wang Dongbo , Gao Shiyong , Wang Jinzhong
Published: February 2021

Recently, different kinds of energy band structures have been utilized to improve the photoelectric properties of zinc oxide (ZnO). In this work, ZnO nanorods were prepared by the hydrothermal method and then decorated with silver sulfide (Ag2S)/zinc sulfide (ZnS) via two-step successive ionic layer adsorption and reaction method. The photoelectric properties of nanocomposites are investigated. The results show that ZnO decorated with Ag2S/ZnS can improve the photocurrent of photodetectors from 0.34 to 0.56 A at bias of 9 V. With the immersion time increasing from 15 to 60 minutes, the photocurrent of photodetectors increases by 0.22 A. The holes in the valence band of ZnO can be transferred to the valence band of ZnS and Ag2S, which promotes the separation and suppresses the recombination of hole-electron pairs generated in ZnO. Moreover, electrons excited by ultraviolet (UV) light in Ag2S can also be injected into the conduction band of ZnO, which causes the photocurrent to increase more than the ZnO photodetector.
Functional and Morphological Changes Induced in Mytilus Hemocytes by Selected Nanoparticles
Authors: Auguste Manon , Mayall Craig , Barbero Francesco , Hočevar Matej , Alberti Stefano , Grassi Giacomo , Puntes Victor , Drobne Damjana , Canesi Laura
Published: February 2021

Nanoparticles (NPs) show various properties depending on their composition, size, and surface coating, which shape their interactions with biological systems. In particular, NPs have been shown to interact with immune cells, that represent a sensitive surveillance system of external and internal stimuli. In this light, in vitro models represent useful tools for investigating nano-bio-interactions in immune cells of different organisms, including invertebrates. In this work, the effects of selected types of NPs with different core composition, size and functionalization (custom-made PVP-AuNP and commercial nanopolystyrenes PS-NH2 and PS-COOH) were investigated in the hemocytes of the marine bivalve Mytilus galloprovincialis. The role of exposure medium was evaluated using either artificial seawater (ASW) or hemolymph serum (HS). Hemocyte morphology was investigated by scanning electron microscopy (SEM) and different functional parameters (lysosomal membrane stability, phagocytosis, and lysozyme release) were evaluated. The results show distinct morphological and functional changes induced in mussel hemocytes depending on the NP type and exposure medium. Mussel hemocytes may represent a powerful alternative in vitro model for a rapid pre-screening strategy for NPs, whose utilization will contribute to the understanding of the possible impact of environmental exposure to NPs in marine invertebrates.
Yersiniosis in New Zealand
Authors: Rivas Lucia , Strydom Hugo , Paine Shevaun , Wang Jing , Wright Jackie
Published: February 2021

The rate of yersiniosis in New Zealand (NZ) is high compared with other developed countries, and rates have been increasing over recent years. Typically, >99% of human cases in NZ are attributed to Yersinia enterocolitica (YE), although in 2014, a large outbreak of 220 cases was caused by Yersinia pseudotuberculosis. Up until 2012, the most common NZ strain was YE biotype 4. The emergent strain since this time is YE biotype 2/3 serotype O:9. The pathogenic potential of some YE biotypes remains unclear. Most human cases of yersiniosis are considered sporadic without an identifiable source. Key restrictions in previous investigations included insufficient sensitivity for the isolation of Yersinia spp. from foods, although foodborne transmission is the most likely route of infection. In NZ, YE has been isolated from a variety of sick and healthy domestic and farm animals but the pathways from zoonotic reservoir to human remain unproven. Whole-genome sequencing provides unprecedented discriminatory power for typing Yersinia and is now being applied to NZ epidemiological investigations. A “One-Health” approach is necessary to elucidate the routes of transmission of Yersinia and consequently inform targeted interventions for the prevention and management of yersiniosis in NZ
In Vitro–In Vivo Correlation (IVIVC) Population Modeling for the In Silico Bioequivalence of a Long-Acting Release Formulation of Progesterone
Authors: Tosca Elena M. , Rocchetti Maurizio , Pérez Elena , Nieto Conchi , Bettica Paolo , Moscoso del Prado Jaime , Magni Paolo , De Nicolao Giuseppe
Published: February 2021

Health authorities carefully evaluate any change in the batch manufacturing process of a drug before and after regulatory approval. In the absence of an adequate in vitro–in vivo correlation (Level A IVIVC), an in vivo bioequivalence (BE) study is frequently required, increasing the cost and time of drug development. This study focused on developing a Level A IVIVC for progesterone vaginal rings (PVRs), a dosage form designed for the continuous delivery in vivo. The pharmacokinetics (PK) of four batches of rings charged with 125, 375, 750 and 1500 mg of progesterone and characterized by different in vitro release rates were evaluated in two clinical studies. In vivo serum concentrations and in vitro release profiles were used to develop a population IVIVC progesterone ring (P-ring) model through a direct differential-equation-based method and a nonlinear-mixed-effect approach. The in vivo release, Rvivo(t), was predicted from the in vitro profile through a nonlinear relationship. Rvivo(t) was used as the input of a compartmental PK model describing the in vivo serum concentration dynamics of progesterone. The proposed IVIVC P-ring model was able to correctly predict the in vivo concentration–time profiles of progesterone starting from the in vitro PVR release profiles. Its internal and external predictability was carefully evaluated considering the FDA acceptance criteria for IVIVC assessment of extended-release oral drugs. Obtained results justified the use of the in vitro release testing in lieu of clinical studies for the BE assessment of any new PVRs batches. Finally, the possible use of the developed population IVIVC model as a simulator of virtual BE trials was explored through a case study.
Performance Evaluation of Highly Nonlinear Fiber (HNLF) Based Optical Phase Conjugation (OPC) in Long Haul Transmission of 640 Gbps 16-QAM CO-OFDM
Authors: Wang Jingjing , Du Yongtao , Liang Chunhao , Li Zhong , Fang Jing
Published: February 2021

This paper presents the quantitative measurement through an experimental test of 640 Gbps 16-QAM coherent-optical orthogonal frequency-division multiplexing (CO-OFDM) over 800 km optical fiber with mid-link optical phase conjugation (OPC) using highly nonlinear fiber (HNLF). The first focus is the OPC parameter optimization, including the optimization of HNLF length and signal/pump power that inputs into OPC. Four different HNLFs, as the illustrative examples, are investigated. The second focus is to investigate the effects of fiber dispersion, nonlinearity, and amplified spontaneous emission (ASE) noise on the long-haul transmission of 16-QAM CO-OFDM signal, and the OPC compensation efficiency. The performance evaluation focuses on the conversion efficiency (CE), received signal constellation, Q-factor improvement, and bit error rate (BER) at the receiver end. Such end-to-end performance evaluation is important because the 16-QAM CO-OFDM signal status is heterogeneous and the mitigation of transmission impairments to the signal is still unclear. The OPC parametric optimization is achieved experimentally using commercially available HNLFs with different scenarios and the numerical results are interpreted in conjunction with simulations.
Photosynthetic Nutrient and Water Use Efficiency of Cucumis sativus under Contrasting Soil Nutrient and Lignosulfonate Levels
Authors: Ikkonen Elena , Chazhengina Svetlana , Jurkevich Marija
Published: February 2021

To reduce the use of commercial conventional inorganic fertilizers, the possibility of using pulp and paper industry wastes in agriculture as an alternative source of nutrients is recently under study and discussion. This work aimed to evaluate the effect of sodium lignosulfonate application to soil on photosynthetic leaf nutrient (N, P, K, Ca, Mg, Fe, Mn, and Na) and water use efficiency. A pot culture experiment was conducted with cucumber seedlings, using five lignosulfonate concentrations (0, 1, 2.5, 5, and 10 vol. %) in sandy soil under sufficient or low nutrient availability for plants. The impact of nutrient availability on the plants’ physiological traits was stronger than the lignosulfonate impact. Under sufficient nutrient availability, the lignosulfonate application resulted in decreased photosynthetic N, P, K, Ca, Mg, Fe, and Na use efficiency. Cucumber growth and development, and photosynthetic nutrient, water, and light use efficiency were significantly reduced with a nutrient deficiency. The sodium lignosulfonate application was not successful in eliminating the negative effects of nutrient deficit on cucumber seedlings.
The Potential of Rhizoctonia-Like Fungi for the Biological Protection of Cereals against Fungal Pathogens
Authors: Bleša Dominik , Matušinský Pavel , Sedmíková Romana , Baláž Milan
Published: February 2021

The use of biological control is becoming a common practice in plant production. One overlooked group of organisms potentially suitable for biological control are Rhizoctonia-like (Rh-like) fungi. Some of them are capable of forming endophytic associations with a large group of higher plants as well as mycorrhizal symbioses. Various benefits of endophytic associations were proved, including amelioration of devastating effects of pathogens such as Fusarium culmorum. The advantage of Rh-like endophytes over strictly biotrophic mycorrhizal organisms is the possibility of their cultivation on organic substrates, which makes their use more suitable for production. We focused on abilities of five Rh-like fungi isolated from orchid mycorrhizas, endophytic fungi Serendipita indica, Microdochium bolleyi and pathogenic Ceratobasidium cereale to inhibit the growth of pathogenic F. culmorum or Pyrenophora teres in vitro. We also analysed their suppressive effect on wheat infection by F. culmorum in a growth chamber, as well as an effect on barley under field conditions. Some of the Rh-like fungi affected the growth of plant pathogens in vitro, then the interaction with plants was tested. Beneficial effect was especially noted in the pot experiments, where wheat plants were negatively influenced by F. culmorum. Inoculation with S. indica caused higher dry shoot biomass in comparison to plants treated with fungicide. Prospective for future work are the effects of these endophytes on plant signalling pathways, factors affecting the level of colonization and surviving of infectious particles.
On Numerical 2D P Colonies Modelling the Grey Wolf Optimization Algorithm
Authors: Valenta Daniel , Langer Miroslav
Published: February 2021

The 2D P colonies is a version of the P colonies with a two-dimensional environment designed for observing the behavior of the community of very simple agents living in the shared environment. Each agent is equipped with a set of programs consisting of a small number of simple rules. These programs allow the agent to act and move in the environment. The 2D P colonies have been shown to be suitable for the simulations of various (not only) multi-agent systems, and natural phenomena, like flash floods. The Grey wolf algorithm is the optimization-based algorithm inspired by social dynamics found in packs of grey wolves and by their ability to create hierarchies, in which every member has a clearly defined role, dynamically. In our previous papers, we extended the 2D P colony by the universal communication device, the blackboard. The blackboard allows for the agents to share various information, e.g., their position or the information about their surroundings. In this paper, we follow our previous research on the numerical 2D P colony with the blackboard. We present the computer simulator of the numerical 2D P colony with the blackboard and the results of the computer simulation, and we compare these results with the original algorithm.
Numerical Investigation of a Novel Plate-Fin Indirect Evaporative Cooling System Considering Condensation
Authors: Zhou Yuanyuan , Yan Zhen , Gao Ming , Dai Qiumin , Yu Yanshun
Published: February 2021

An indirect evaporative cooling system combining with thermoelectric cooling technology (i.e., TIEC system) is proposed, in which a counter-flow plate-fin indirect evaporative cooler is inserted with thermoelectric cooling (i.e., TEC) modules. In hot and humid climate, condensation may occur on the dry channel surface of the cooler. For the TIEC system, with the aid of TEC technology, the surface temperature of the dry channel can be much lower than that of a traditional indirect evaporative cooler, thus, the condensation from the primary air is more likely to take place. A numerical model of this novel TIEC system is developed with specifically taking condensation from primary air into account. Detailed performance analysis of the TIEC system is carried out. Analytical results found that the condensation from primary air reduces the dew point effectiveness by up to 45.0% by weakening the sensible heat transfer but increases the coefficient of performance by up to 62.2% by increasing the latent heat transfer, under given conditions. The effects of main operating conditions, such as the electrical current I and number n of TEC modules, inlet temperature Tp,i, humidity ratio RHp and velocity Vp of the primary air, and the mass flow rate ratio x of secondary to primary air, are investigated under non-condensation and condensation states. It is shown that condensate is more easily produced under higher I, n, Tp,i, RHp, x and lower Vp.
An Overview of Plastic Waste Generation and Management in Food Packaging Industries
Authors: Ncube Lindani Koketso , Ude Albert Uchenna , Ogunmuyiwa Enoch Nifise , Zulkifli Rozli , Beas Isaac Nongwe
Published: February 2021

Over the years, the world was not paying strict attention to the impact of rapid growth in plastic use. This has led to unprecedented amounts of mixed types of plastic waste entering the environment unmanaged. Packaging plastics account for half of the global total plastic waste. This paper seeks to give an overview of the use, disposal, and regulation of food packaging plastics. Demand for food packaging is on the rise as a result of increasing global demand for food due to population growth. Most of the food packaging are used on-the-go and are single use plastics that are disposed of within a short space of time. The bulk of this plastic waste has found its way into the environment contaminating land, water and the food chain. The food industry is encouraged to reduce, reuse and recycle packaging materials. A wholistic approach to waste management will need to involve all stakeholders working to achieve a circular economy. A robust approach to prevent pollution today rather than handling the waste in the future should be adopted especially in Africa where there is high population growth.
Surviving the “Sexplosion”: Christianity Today and Evangelical Sexual Ethics in the Long 1960s
Authors: Pattillo-Lunt Aaron
Published: February 2021

This paper examines how the editors and contributors to Christianity Today (CT) called for an evangelical sexual ethics in the 1960s. Editors and contributors alike were concerned that the supposed sexual immorality on college campuses, the liberalization of obscenity laws, the approval and sale of the birth control, and secular sex education programs threatened the United States’ social health. They believed that evangelicals needed to learn how to talk about sex, and this belief resulted in the development of conservative Protestant sex manuals by the middle of the 1970s. Overall, talk about sex in the pages of CT demonstrates that evangelicals are neither anti-sex nor traditionalists. They instead forged a new sexual ethic in response to the historical events and developments of the 1960s.
Similarities and Differences between Danish and American Physicians’ Religious Characteristics and Clinical Communication: Two Cross-Sectional Surveys
Authors: van Randwijk Christian B. , Opsahl Tobias , Assing Hvidt Elisabeth , Stripp Tobias Kvist , Bjerrum Lars , Herrstedt Jørn , Søndergaard Jens , Hvidt Niels Christian
Published: February 2021

Many physicians remain reticent to initiate or partake in discussions about their patients’ religious and spiritual needs during the clinical encounter. Reasons for this may be insufficient time, capacity, education or training but may also be a product of variance in physicians’ own religious or spiritual characteristics. The aim of this paper was to compare American and Danish physicians’ religious characteristics, and to explore and compare American and Danish physicians’ attitudes towards, and practices of, integrating religiosity and spirituality in the clinical encounter. We included data from two cross-sectional surveys: an American survey conducted in 2002 (n = 2000) and a Danish survey conducted in 2012 (n = 1485) to test four hypotheses. American physicians were significantly more religious, they more frequently inquired about religious or spiritual issues in the clinical encounter and they found it more appropriate to discuss religious or spiritual issues if the patients brought it up when compared to Danish physicians. A weak to moderate positive correlation between level of religiosity and frequency of inquiring about religious and spiritual issues were found in both populations. The findings are discussed in relation to the clinical importance of ensuring that health care practices stay patient centered. The findings may especially be relevant to consider in increasingly ethnically and culturally diverse contexts.
Adaptive Position/Force Control of a Robotic Manipulator in Contact with a Flexible and Uncertain Environment
Authors: Gierlak Piotr
Published: February 2021

The present paper concerns the synthesis of robot movement control systems in the cases of disturbances of natural position constraints, which are the result of surface susceptibility and inaccuracies in its description. The study contains the synthesis of control laws, in which the knowledge of parameters of the susceptible environment is not required, and which guarantee stability of the system in the case of an inaccurately described contact surface. The novelty of the presented solution is based on introducing an additional module to the control law in directions normal to the interaction surface, which allows for a fluent change of control strategy in the case of occurrence of distortions in the surface. An additional module in the control law is perceived as a virtual viscotic resistance force and resilient environment acting upon the robot. This interpretation facilitates intuitive selection of amplifications and allows for foreseeing the behavior of the system when disturbances occur. Introducing reactions of virtual constraints provides automatic adjustment of the robot interaction force with the susceptible environment, minimizing the impact of geometric inaccuracy of the environment.