bluuebunny/crossref_metadata_2025 huggingface dataset:
A slow V̇O2 on-response allows to comfortably adopt aerobically unaffordable walking and running speeds in short stairs ascending
Authors: Minetti Alberto E. , Rapuzzi Fabrizio , Alberton Cristine Lima , Pavei Gaspare
Published: August 2020

The aim of this paper is to investigate the mechanical and metabolic reasons of the spontaneous gait/speed choice of ascending short flight of stairs, where walking on every step or running on every other step are frequently interchangeable options. Twenty-four subjects' kinematics, oxygen uptake (V̇O2), ventilation and heart rate were sampled during climbing one and two flights of stairs while using the two gaits. Although motor acts were very short in time (5-22 s), metabolic kinetics, extending in the successive 250 s after the end of climbing, consistently reflected the (equivalent of the) needed mechanical energy and allowed to compare the two ascent choices: despite a 250% higher mechanical power associated to running, measured V̇O2, ventilation and heart rate peaked only at +25% with respect to walking, and in both gaits at a much lower values than V̇O2max despite of predictions based on previous gradient locomotion studies. Mechanical work and metabolic cost of transport, as expected, showed similar increase (+25%) in running. For stairs up to 4.8 m tall (30 steps at 53% gradient), running makes us consuming slightly more calories than walking, and in both gaits at no discomfort at all. The cardio-respiratory-metabolic responses similarly delay and damp the replenishing of phosphocreatine stores, which were much faster depleted during the impulsive, highly powered mechanical event, with almost overlapping time courses. Such a discrepancy between mechanical and metabolic dynamics allows to afford almost-to-very anaerobic climbs and to interchangeably decide whether to walk or run up a short flight of stairs.
Measuring power input, power output and energy conversion efficiency in un-instrumented flying birds
Authors: Hedh Linus , Guglielmo Christopher G. , Johansson L. Christoffer , Deakin Jessica E. , Voigt Christian C. , Hedenström Anders
Published: August 2020

Cost of flight at various speeds is a crucial determinant of flight behavior in birds. Aerodynamic models, predicting that mechanical power (Pmech) varies with flight speed in a U-shaped manner, have been used together with an energy conversion factor (efficiency) to estimate metabolic power (Pmet). Despite few empirical studies, efficiency has been assumed constant across flight speeds at 23%. Ideally, efficiency should be estimated from measurements of both Pmech and Pmet in un-instrumented flight. Until recently, progress has been hampered by methodological constraints. The main aim of this study was to evaluate recently developed techniques and estimate flight efficiency across flight speeds. We used the 13C-labeled sodium bicarbonate method (NaBi) and Particle Image Velocimetry (PIV) to measure Pmet and Pmech in blackcaps flying in a wind tunnel. We also cross validated measurements made by NaBi with Quantitative Magnetic Resonance (QMR) body composition analysis in yellow-rumped warblers. We found that Pmet estimated by Nabi was ∼12% lower than corresponding values estimated by QMR. Pmet varied in a U-shaped manner across flight speeds in blackcaps, but the pattern was not statistically significant. Pmech could only be reliably measured for two intermediate speeds and estimated efficiency ranged between 14 and 22% (combining the two speeds for raw and weight/lift specific power, with and without correction for the ∼12% difference between NaBi and QMR) were close to the currently used default value. We conclude that NaBi and PIV are viable techniques, allowing researchers to address some of the outstanding questions regarding bird flight energetics.
3′READS + RIP defines differential Staufen1 binding to alternative 3′UTR isoforms and reveals structures and sequence motifs influencing binding and polysome association
Authors: Zheng Dinghai , Cho Hana , Wang Wei , Rambout Xavier , Tian Bin , Maquat Lynne E.
Published: August 2020

Staufen1 (STAU1) is an RNA-binding protein (RBP) that interacts with double-stranded RNA structures and has been implicated in regulating different aspects of mRNA metabolism. Previous studies have indicated that STAU1 interacts extensively with RNA structures in coding regions (CDSs) and 3′-untranslated regions (3′UTRs). In particular, duplex structures formed within 3′UTRs by inverted-repeat Alu elements (IRAlus) interact with STAU1 through its double-stranded RNA-binding domains (dsRBDs). Using 3′ region extraction and deep sequencing coupled to ribonucleoprotein immunoprecipitation (3′READS + RIP), together with reanalyzing previous STAU1 binding and RNA structure data, we delineate STAU1 interactions transcriptome-wide, including binding differences between alternative polyadenylation (APA) isoforms. Consistent with previous reports, RNA structures are dominant features for STAU1 binding to CDSs and 3′UTRs. Overall, relative to short 3′UTR counterparts, longer 3′UTR isoforms of genes have stronger STAU1 binding, most likely due to a higher frequency of RNA structures, including specific IRAlus sequences. Nevertheless, a sizable fraction of genes express transcripts showing the opposite trend, attributable to AU-rich sequences in their alternative 3′UTRs that may recruit antagonistic RBPs and/or destabilize RNA structures. Using STAU1-knockout cells, we show that strong STAU1 binding to mRNA 3′UTRs generally enhances polysome association. However, IRAlus generally have little impact on STAU1-mediated polysome association despite having strong interactions with the protein. Taken together, our work reveals complex interactions of STAU1 with its cognate RNA substrates. Our data also shed light on distinct post-transcriptional fates for the widespread APA isoforms in mammalian cells.
Low Back Pain in Operating Room Nurses and Its Associated Factors
Authors: Cavdar Ikbal , Karaman Ahmet , Ozhanlı Yasemin , Ozbas Ayfer
Published: August 2020

Objective: To determine the presence of low back pain and the associated factors in operating room nurses.

Methods: The population of the descriptive study consists of 133 operating room nurses working in the operating rooms of five major hospitals located in Istanbul, and the study sample consists of 96 operating room nurses who are not on leave or sick leave between July-2016 to February 2017. Data were collected via a question form prepared by the researchers.

Results: It was determined that more than half of the operating room nurses forming the sample group had low back pain and that it is affected from the practices of operating room nurses during a shift, which may cause physical strain such as year of working as an operating room nurse, bending and staying in the same position for a long time, holding an instrument for a long time, rotational movement inadequate to body mechanics, lifting/carrying heavy medical items and pushing/pulling heavy medical equipment.

Conclusion: Majority of operating room nurses had low back pain and it was associated with coercive movements during surgery.

doi: https://doi.org/10.12669/pjms.36.6.2836

How to cite this:Cavdar I, Karaman A, Ozhanli Y, Ozbas A. Low Back Pain in Operating Room Nurses and Its Associated Factors. Pak J Med Sci. 2020;36(6):1291-1296. doi: https://doi.org/10.12669/pjms.36.6.2836

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Case Report: Complex parapneumonic effusion and bacteremia secondary to Granulicatella adiacens in immunocompetent host
Authors: Acharya Ajita , Sattar Zeeshan , Ahmad Ammar , Sattar Farhan , Fiksman Aleksey
Published: August 2020

Granulicatella adiacens is a fastidious Gram-positive streptococcus that is linked with causing a variety of infections in immunocompromised hosts. These include endocarditis, bacteremia, osteomyelitis, and spontaneous bacterial peritonitis. We present a rare incidence of this bacterium causing a complex parapneumonic effusion in an immunocompetent host. An effective antibiotic regimen for treating infections caused by this bacterium includes ampicillin plus penicillin plus gentamicin but resistance to various classes of antibiotics has been reported. In conclusion, this organism can affect different systems in immunocompromised as well as immunocompetent hosts, and a high index of suspicion is required for its diagnosis.
Liderazgo transformacional y equidad de género: el caso de estudiantes de posgrado
Authors: Díaz Gómez Eduardo Raúl
Published: August 2020

La representación de la mujer en roles de liderazgo incrementa en contextos organizacionales, educativos y políticos en México. Sin embargo, todavía existen barreras para la inclusión y equidad de género para mujeres que aspiran a desempeñarse como líderes. Estas barreras parten de estereotipos que relacionan los estilos directivos de la mujer como comunales que, si bien fomentan el desarrollo de relaciones interpersonales, merman el cumplimiento de objetivos y el alto desempeño de equipos de trabajo que responden mejor a estilos gerenciales tradicionalmente relacionados con el género masculino. Existen estudios empíricos desarrollados con base en la teoría de liderazgo transformacional que desmienten que mujeres y hombres actúan diferente en puestos de liderazgo. La característica universal de la teoría transformacional de liderazgo la hace especialmente apropiada para comparar conductas entre grupos de género femenino y masculino, especialmente en estudios donde se adopta un diseño de auto-reporte. Este estudio utiliza un modelo de liderazgo transformacional para comparar la autopercepción de 309 estudiantes de posgrado entre mujeres y hombres. Los resultados sugieren que ambos grupos perciben su comportamiento de manera similar. Este resultado complementa otros estudios donde se sostiene que el género no influye sobre la conducta de líderes transformacionales.
Shaping Markets Through Temporal, Constructive, and Interactive Agency
Authors: Pontikes Elizabeth G. , Rindova Violina P.
Published: August 2020

In this introductory essay, we develop a theoretical framework of agency as a basis for strategic shaping and market transformation. We conceive of agency as both constrained and enabled by structure, and we build on sociological views that treat market structures as pairings of cultural schemas and material resources that are mutually sustaining. Structures contain the seeds for change because contradictions and conflicts that are inherent to structure inspire agents to imagine a new order and provide pathways to enact them. We theorize three connected forms of agency. Constructive agency captures agents’ ability to differently apply schemas to mobilize resources and improve their strategic positions. Temporal agency underlies agents’ autonomy and individuation, and enables agents to envision new possibilities. Interactive agency captures the collective nature of agency, where interactions among heterogeneous actors provide opportunities for agents to persuade others of their changing conceptions and learn new schemas, expanding agents’ repertoires for shaping opportunities.
CONTRIBUIÇÕES PARA UMA GEOGRAFIA DA VIDA COTIDIANA: JOÃO DO RIO, UM FLÂNEUR DOS TRÓPICOS
Authors: Soares Anael Ribeiro
Published: August 2020

O presente estudo se propõe a analisar parte do que foi produzido por João do Rio entre o fim séc. XIX e o início do XX como contribuições aos estudos da Geografia urbana brasileira, tendo como recorte espacial a metrópole carioca sob o véu da belle époque, a partir de uma categoria de análise central: a vida cotidiana. O arsenal teórico-metodológico no qual se buscou      subsídios estão associados aos estudos no interior da teoria crítica. Ao final, ao aproximarmos a obra de João do Rio com a as condições objetivas no espaço-tempo a partir das quais sua arte ganhava corporeidade, observando-se o lugar que ocupa o cotidiano em suas crônicas, bem como papel da rua enquanto lócus dos encontros.            Palavras-chave: Arte; João do Rio; Vida Cotidiana.
Assessing capacity in cases of fluctuating decision-making ability
Authors: Griffith Richard
Published: August 2020

Richard Griffith, Senior Lecturer in Health Law at Swansea University, considers the law on decision-making capacity and recent approaches to the assessment of fluctuating capacity by the Court of Protection
Implementation of spiritual care in patients with diabetic foot ulcers: a literature review
Authors: Subrata Sumarno Adi
Published: August 2020

Lower extremity amputation is a complication of diabetic foot ulcers that can cause spiritual crisis. Integrating spiritual care into nursing practice is important to overcome this. However, studies articulating the role of nurses in spiritual care when caring for patients with diabetic foot ulcers is limited. This article aims to describe the importance of providing spiritual care for this group of patients. The interpretation of spiritual care with respect to the relationship between the patient and the family, and the role of the nurse are discussed. The findings offer a theoretical perspective on spiritual care that can be used to develop spiritual interventions, as well as prevent spiritual crises in patients with diabetic foot ulcers.
Independent non-medical prescribing in a nurse-led hospice: strengths and challenges
Authors: Dawson Sheila
Published: August 2020

Independent non-medical prescribing has been a safe, effective and economical initiative for St Teresa's nurse-led hospice, as indicated by yearly audits of prescribing charts and incident reports. However, there are several challenges involved, some shared with other palliative care organisations and some unique to this hospice. These include accessing consistent evidence, especially in light of high volumes of off-licence or unlicensed prescribing with limited stock of medication, and pro-actively pursuing tailored Continuous Professional Development. These challenges, though sometimes complex, are not insurmountable for the experienced non-medical prescribers in this non-conventional setting.
Mueller matrix polarimeter based on twisted nematic liquid crystal devices
Authors: Chironi Emanuel , Iemmi Claudio
Published: August 2020

We present a precise, portable, and inexpensive Mueller matrix polarimeter based on a pair of twisted nematic liquid crystal plates. Such a design is of interest to low-budget users or to those who require a portable instrument for applications to be carried on outside a laboratory environment. We develop the theoretical model for the proposed polarimeter and describe the complete implementation of the device. This process includes the selection of the optimum states of polarization to perform the measurements, the calibration of the instrument, and the evaluation of its performance for punctual and imaging applications. The proposed device is at least as accurate and precise as similar, but much more expensive, polarimeters.
Deep end-to-end rolling shutter rectification
Authors: Kandula Praveen , Kumar T. Lokesh , Rajagopalan A. N.
Published: August 2020

CMOS sensors employ a row-wise acquisition mechanism while imaging a scene, which can result in undesired motion artifacts known as rolling shutter (RS) distortions in the captured image. Existing single image RS rectification methods attempt to account for these distortions by using either algorithms tailored for a specific class of scenes that warrants information of intrinsic camera parameters or a learning-based framework with known ground truth motion parameters. In this paper, we propose an end-to-end deep neural network for the challenging task of single image RS rectification. Our network consists of a motion block, a trajectory module, a row block, an RS rectification module, and an RS regeneration module (which is used only during training). The motion block predicts the camera pose for every row of the input RS distorted image, while the trajectory module fits estimated motion parameters to a third-order polynomial. The row block predicts the camera motion that must be associated with every pixel in the target, i.e., RS rectified image. Finally, the RS rectification module uses motion trajectory and the output of a row block to warp the input RS image to arrive at a distortion-free image. For faster convergence during training, we additionally use an RS regeneration module that compares the input RS image with the ground truth image distorted by estimated motion parameters. The end-to-end formulation in our model does not constrain the estimated motion to ground truth motion parameters, thereby successfully rectifying the RS images with complex real-life camera motion. Experiments on synthetic and real datasets reveal that our network outperforms prior art both qualitatively and quantitatively.
Surface plasticizing of chalcogenide glasses: a route for direct nanoimprint with multifunctional antireflective and highly hydrophobic structures
Authors: Tzadka Sivan , Ostrovsky Natali , Toledo Esti , Saux Guillaume Le , Kassis Evyatar , Joseph Shay , Schvartzman Mark
Published: August 2020

Chalcogenide glasses are attractive materials for optical applications. However, these applications often require pattering of the surface with functional micro-/ nanostructures, which is challenging by traditional microfabrication. Here, we present a novel, robust, and scalable approach for the direct patterning of chalcogenide glasses, based on soft imprinting of a solvent-plasticized glass layer formed on the glass surface. We established a methodology for surfaces plasticizing, through tuning of its glass transition temperature by process conditions, without compromising on the chemical composition, structure, and optical properties of the plasticized layer. This control over the glass transition temperature allowed to imprint the surface of chalcogenide glass with features sized down to 20 nm, and achieve an unprecedented combination of full pattern transfer and complete maintenance of the shape of the imprinted substrate. We demonstrated two applications of our patterning approach: a diffraction grating, and a multifunctional pattern with both antireflective and highly hydrophobic water-repellent functionalities – a combination that has never been demonstrated for chalcogenide glasses. This work opens a new route for the nanofabrication of optical devices based on chalcogenide glasses and paves the way to numerous future applications for these important optical materials.
Exploring performance of THz metamaterial biosensor based on flexible thin-film
Authors: Wang Zhaoyang , Geng Zhaoxin , Fang Weihao
Published: August 2020

To extend the application of flexible metamaterial in the biosensor field, a metamaterial biosensor, which consisted of metal elliptical split-ring resonator array with a subwavelength structure based on flexible thin-film (parylene-c), was presented. The structure parameters (ring width, period ratio of structure, gap width, axial ratio) of the elliptical split-ring resonator and polarization direction of incident light were investigated as to how to affect the performances of the flexible metamaterial biosensor. Meanwhile, the permittivity (ε) of the tested sample on the surface of metamaterials biosensor also affected the shift of transmission spectra. The results showed that the sensitivity, quality (Q) factor, and figure of merit (FOM) of the flexible metamaterial biosensor could reach 243 GHz/RIU, 14.2, and 3.3, respectively. Moreover, the full-width-half-maximum (FWHM) was only 82 GHz. Therefore, these results provided an improved direction to design metamaterial biosensors with high Q-factor, low FOM, and high sensitivity, which could meet the need for sample detection in the terahertz regime.
Fiber micro-tip temperature sensor based on cholesteric liquid crystal
Authors: Hu Jianyang , Zhou Dong , Su Yueming , Liu Shuangqiang , Miao Peixian , Shi Yanchao , Sun Weimin , Liu Yongjun
Published: August 2020

This Letter proposes and demonstrates a novel, miniature fiber-tip temperature sensor with a tapered hollow capillary tube (HCT) filled with glycerin and dye-doped cholesteric liquid crystal (CLC). The function of glycerin is to provide a surface anchoring force to control the uniform orientation of CLC molecules, so that the CLC in the tapered HCT can be considered as a mirrorless photonic bandgap (PBG) microcavity. An unambiguously identifiable PBG mode single peak appears in the emission spectra of the sensor. The CLC-based fiber-tip temperature sensor has a temperature sensitivity of

−

9.167

n
m

/

∘

C

, and the figure of merit can reach

67.4
∘

C

−
1

. This sensor offers key features and advantages, including compactness, unambiguous identifiability, and biocompatibility, which can satisfy requirements of temperature measurement in various temperature sensing application fields and has great potential for biochemical detection at cell level. In addition, the CLC was integrated into the optical fiber terminal, and the PBG mode is excited, collected and transmitted by the multimode fiber coupler, which is reported for the first time, to the best of our knowledge.
Stochastic non-Hermitian skin effect
Authors: Longhi Stefano
Published: August 2020

A hallmark of photonic transport in non-Hermitian lattices with asymmetric hopping is the robust unidirectional flow of light, which is responsible for important phenomena such as the non-Hermitian skin effect. Here we show that the same effect can be induced by stochastic fluctuations in lattices that maintain a symmetric hopping on average. We illustrate such a fluctuation-induced non-Hermitian transport by discussing stochastic funneling of light, in which light is pushed toward an interface by the stochastic-induced skin effect.
In-line ultra-thin attosecond delay line with direct absolute-zero delay reference and high stability
Authors: Dahiya Sunil , Sidhu Mehra S. , Tyagi Akansha , Mandal Ankur , Nandy Biplob , Rost Jan M. , Pfeifer Thomas , Singh Kamal P.
Published: August 2020

We introduce an ultra-thin attosecond optical delay line based on controlled wavefront division of a femtosecond infrared pulse after transmission through a pair of micrometer-thin glass plates with negligible dispersion effects. The time delay between the two pulses is controlled by rotating one of the glass plates from absolute zero to several optical cycles, with 2.5 as to tens of attosecond resolution with 2 as stability, as determined by interferometric self-calibration. The performance of the delay line is validated by observing attosecond-resolved oscillations in the yield of high harmonics induced by time delayed infrared pulses, in agreement with a numerical simulation for a simple model atom. This approach can be extended in the future for performing XUV-IR attosecond pump–probe experiments.
PHARMACOLOGICAL APPLICATIONS OF CONTROLLED RELEASE DRUGS
Authors: Melo Vinicius Farias de , Coelho José Leonardo Gomes , Sousa Carmelita Maria Silva , Fernandes Elis Augusta Moura Dias , Brito Rivaniery Teles Braga Emídio de , Santana Willma José de
Published: August 2020

Abstract: This present aims to discuss the main systems of controlled release of drugs and their pharmacological applications. For this purpose, this study was based on articles between the years 2016 to 2020 in English, Spanish and Portuguese, using the following descriptors: Pharmacological applications, Controlled release, Conventional release. The main systems discussed were polymeric nanoparticles, liposomes and cyclodextrins. These systems guarantee a constant concentration of the drug in the sage and promote a dose comfort to the patient, minimizing the amount of administration, unexpected effects. Keywords: Pharmacological applications, Controlled release, Conventional release.
Do signo ao significado
Authors: Bertucci Roberlei Alves
Published: August 2020

Resenha referente à obra: FERRAREZI JR., Celso. Semântica. São Paulo: Parábola, 2019. Coleção Linguística para o Ensino Superior. Vol. 6.
Plates
Authors: Wangsgaard Jürgensen Martin
Published: August 2020

This volume presents a thorough study of the more than a thousand preserved Danish medieval rural parish churches. It traces the transformations of church interiors from c. 1450 to 1600 (thus covering both the emergence and impact of the Danish Reformation) by interpreting material changes within a broad historical perspective that highlights changes in religious practices and liturgy. The book explores the spatial and artistic implications of liturgy as well as the role of the congregation, the donor, and the clergy both in shaping and disrupting these interiors. It sets out to answer four basic questions: What did these rural churches look like by the middle of the fifteenth century? How did they change from the middle of the fifteenth century to the end of the sixteenth? How were they used and integrated into public as well as private ceremonies? And how may these churches have been perceived and experienced by the congregation and clergy?

This study seeks to establish a methodological framework that incorporates the disciplines of archaeology, art history, history, and theology, in order to facilitate an overall understanding of the architectural setting, embracing spatial, material, and artistic elements within the church through liturgy.
Saliva proteomic analysis reveals possible biomarkers of renal cell carcinoma
Authors: Zhang Xiao Li , Wu Zheng Zhi , Xu Yun , Wang Ji Guo , Wang Yong Qiang , Cao Mei Qun , Wang Chang Hao
Published: August 2020

Early diagnosis is a key to improve the prognosis of renal cell carcinoma (RCC); however, reliable RCC biomarkers are lacking in clinical practice. In this study, we used isobaric tags for relative and absolute quantification-based mass spectrometry to identify salivary proteins as biomarkers for the diagnosis of RCC. The objective of this study is to discover biomarkers from saliva by utilizing high-throughput quantitative proteomics approaches. Saliva proteins from 124 RCC patients and healthy individuals were identified and quantified. RCC putative biomarkers were verified by real-time polymerase chain reaction or enzyme-linked immunosorbent assay in a prevalidation sample set. Seventy-one differentially expressed salivary proteins were identified. Serotransferrin, haptoglobin, KRT9, and S100A9, which in previous studies were found to be most closely related to cancers, were selected as putative RCC biomarkers. Haptoglobin and S100A9 were significantly elevated in RCC compared with healthy control samples, although the expression of serotransferrin and KRT9 did not differ between the groups. Furthermore, receiver operating characteristic curves with a cut-off value of 75.49 ng/mL for S100A9 revealed a sensitivity of 87.10% and a specificity of 91.94% for discriminating RCC patients from healthy individuals. Salivary haptoglobin differentiated RCC patients from healthy controls with a sensitivity of 85.48% and specificity of 80.65% (cut-off value 43.02 µg/mL). These results provide experimental evidence to support S100A9 and haptoglobin as potential novel, noninvasive biomarkers for the diagnosis of RCC.
Withania frutescens: Chemical characterization, analgesic, anti-inflammatory, and healing activities
Authors: EL Moussaoui Abdelfattah , Jawhari Fatima Zahra , Bourhia Mohammed , Maliki Imane , Sounni Fatiha , Mothana Ramzi A. , Bousta Dalila , Bari Amina
Published: August 2020

Withania frutescens (W. frutescens) is a medicinal plant that is largely used in the Morrocan pharmacopeia for disease treatment. This work was conducted to investigate the chemical characterization, analgesic, anti-inflammatory, and healing activities of W. frutescens. The chemical characterization of W. frutescens extract was done using HPLC; the anti-inflammatory test was performed with doses 300, 400 and 450 mg/kg, and the healing activity was assessed using two creams (extract 5% and extract 10%). Phytochemical analysis revealed the presence of phenolic compounds. The results of the anti-inflammatory test were more pronounced when compared with the reference drug with a maximum inhibition percentage of 82.20% ± 8.69 obtained at the dose of 450 mg/kg. Local application of 10% plant cream induced 80.17% ± 7.89 of inflammation inhibition when compared with the indomethacin drug 92.33% ± 11.27. The studied plant extract showed a promising healing activity with the following percentage: 99.03% ± 0.76 (extract 10%), 98.61% ± 1.91 (extract 5%), and 57.43% ± 2.97 (control); meanwhile, the value reached to 100% ± 0.02 for the drug that was used as a reference within the first 2 weeks. The plant studied in this work would be a promising source for conceptualizing effective drugs against inflammatory diseases.
Development and validation of a high performance liquid chromatography/diode array detection method for estrogen determination: Application to residual analysis in meat products
Authors: Alqahtani Sadeem S. , Bin Humaid Deema M. , Alshail Sabreen H. , AlShammari Dalal T. , Al-Showiman Hessa , Alzoman Nourah Z. , Maher Hadir M.
Published: August 2020

In this work, an HPLC-DAD method was developed for the residual analysis of some estrogens such as estrone (E1), 17-β estradiol (E2), estriol (E3), natural estrogens, and 17-α ethinylestradiol (E4), an exoestrogen, in meat samples of different categories (chicken, n = 155, beef, n = 124, sheep, n = 122, and camels, n = 40), collected from the Saudi Market. Although banned, the use of E4 as a growth promoter in the black market is still encountered. Symmetry C18 column (3.5 µm, 4.6 mm × 150 mm) was used with a mobile phase consisting of 50% aqueous acetonitrile. Protein precipitation with acetonitrile was used for the sample preparation. The method was fully validated, as per the ICH guidelines, in the concentration ranges of 0.35–125 µg/g (E1, E2), 0.188–125 µg/g (E3), and 0.188–450 µg/g (E4). The method allowed the trace analysis of estrogens with LOD values of 0.094 (E3, E4) and 0.126 µg/g (E1, E2), and LOQ values of 0.188 (E3, E4) and 0.350 µg/g (E1, E2). The analyzed samples contained different levels of estrogens. Within the same category, processed products contained the highest levels of E4, while the internal organs contained the least estrogen content. Finally, the estimated daily intake, µg/kg bw/day, of estrogens through the consumption of meat-based food products was calculated.
A theoretical and experimental study of the adsorptive removal of hexavalent chromium ions using graphene oxide as an adsorbent
Authors: Alija Ardhmeri , Gashi Drinisa , Plakaj Rilinda , Omaj Admir , Thaçi Veprim , Reka Arianit , Avdiaj Sefer , Berisha Avni
Published: August 2020

This study is focused on the adsorption of hexavalent chromium ions Cr(vi) using graphene oxide (GO). The GO was prepared by chemical oxidation (Hummers method) of graphite particles. The synthesized GO adsorbent was characterized by Fourier transform infrared spectroscopy and UV-Vis spectroscopy. It was used for the adsorption of Cr(vi) ions. The theoretical calculations based on density functional theory and Monte Carlo calculations were used to explore the preferable adsorption site, interaction type, and adsorption energy of GO toward the Cr(vi) ions. Moreover, the most stable adsorption sites were used to calculate and plot noncovalent interactions. The obtained results are important as they give molecular insights regarding the nature of the interaction between GO surface and the adsorbent Cr(vi) ions. The found adsorption energy of −143.80 kcal/mol is indicative of the high adsorptive tendency of this material. The adsorption capacity value of GO toward these ions is q = 240.361 mg/g.
Optimisation of urine sample preparation for shotgun proteomics
Authors: Tkáčiková Soňa , Talian Ivan , Sabo Ján
Published: August 2020

Urine reflects the renal function and urinary and kidney systems, but it may also reflect the presence of cancer in other parts of the body. Urine also has potential for providing prognostic information during therapeutic treatments thanks to non-invasive monitoring. A quick and reproducible protein purification procedure is essential to allow data comparison between proteomic studies in urine biomarker discovery. The article describes a simple, reproducible and cheap sample preparation procedure with a maximum protein yield (400 µg) obtained from only 10 mL of urine utilising cut-off filter desalting and digestion. The reported procedure removes yellowish background coloration residues and thus prevents the errors in spectrophotometric protein concentration determination. Different extraction solvents used in the presented procedure point to the possibility of partial elimination of abundant proteins (albumin and keratin family), as well as to the improvement of the sequence coverage of proteins identified, which helps to reveal changes in the urinary proteome. With this workflow, proteins can be easily obtained on standard laboratory equipment within 3 h. Data are available via ProteomeXchange with identifier PXD019738.
TIC en el sector público del Sistema de Salud de México: Avances y oportunidades
Authors: Díaz de León Castañeda Christian
Published: August 2020

Las tecnologías de información y comunicación (TIC) son una oportunidad de innovación y mejora para los sistemas de salud. Las diferentes aplicaciones de las TIC en el campo de la salud se han asociado al término salud electrónica (e-Salud) o salud digital y han mostrado ser costo-efectivas y favorecer el desempeño de los sistemas de salud. Tomando como base el marco conceptual de las funciones básicas de los sistemas de salud (rectoría/gobernanza, financiamiento, generación de recursos y provisión de servicios), se realizó un análisis de avances y oportunidades de la e-Salud en el sistema de salud de México. Este análisis se basó principalmente en una revisión narrativa de la literatura y entrevistas semiestructuradas. En el estudio se identifican avances importantes en e-Salud en México; sin embargo, aún quedan muchos pendientes por resolver, principalmente a nivel de fortalecimiento de las políticas, así como mejora en el acceso e implementación de estas tecnologías.
H/D isotope effect and magnetic properties of cyanide-bridged Nd(III)-Fe(III) complex
Authors: Akitsu T. , Kimoto Y. , Yamada Y. , Nomura K.
Published: August 2020

We have prepared four H/D isotope forms of Nd(DMF)4(H2O)3Fe(CN)6·H2O (DMF = N,N-dimethylformamide) by using D2O or DMF-d1. Temperature dependence of magnetization exhibits H/D isotope effects resulting from intermolecular hydrogen bonds. Temperature dependence of Fe 2p
3/2 and 2p
1/2 XAS and 57Fe Mössbauer spectra suggested that the influence of coordination environment could be distiguished from the influence of crystal lattice based on preliminary crystallographic results.
Meiotic Double-Strand Break Processing and Crossover Patterning Are Regulated in a Sex-Specific Manner by BRCA1–BARD1 in Caenorhabditis elegans
Authors: Li Qianyan , Hariri Sara , Engebrecht JoAnne
Published: August 2020

Sperm and oocyte production are differentially regulated to ensure genetic information is accurately passed down from one generation to the next.....
Mixoma odontogénico, presentación de caso y revisión de literatura
Authors: Jerez Robalino James , Salgado Chavarría Fabiola , Lucio Leonel Ernesto , Olmedo Cueva Sergio
Published: August 2020

El mixoma odontogénico es una lesión benigna que se presenta mayormente entre la segunda y cuarta década de vida, con mayor frecuencia en zona de molares y mayor incidencia en hombres que en mujeres (2:1). Histológicamente presenta células estrelladas y fusiformes dispersas en una matriz de aspecto mixoide muy abundante. El objetivo de este artículo es reportar un caso clínico de un mixoma odontogénico, así como una breve revisión de la literatura. Una paciente mujer de 39 años que acudió a la clínica de Cirugía Oral y Maxilofacial por presentar aumento de volumen localizado en mandíbula en zona vestibular entre dientes 31 y 44, asintomática a la palpación, con una evolución de aproximadamente doce meses. Se decidió realizar una biopsia incisional para el estudio histopatológico. El resultado del estudio histopatológico fue mixoma odontogénico por lo que se realizó el tratamiento de resección con márgenes libres y colocación de placa de reconstrucción, así como controles posoperatorios.
Miositis osificante del músculo pterigoideo medial secundaria a enucleación de quiste dentígero: reporte de caso
Authors: Jiménez Zúñiga Fidel , Medecigo Costeira Daniel , Varela Ibáñez Carlos Eduardo , Miranda Villasana José Ernesto
Published: August 2020

La miositis osificante es una rara entidad caracterizada por depósito de hueso heterotópico en tejidos blandos, siendo su principal ubicación el músculo, se han descrito dos variantes: miositis osificante progresiva y miositis osificante traumatica (MOT). El objetivo de este artículo es reportar el primer caso de MOT diagnosticado en México, en paciente femenina de 11 años de edad, quien posterior a enucleación de quiste dentígero desarrolló limitación progresiva a la apertura oral, dolor y trismus, al examen físico sin palpación de masas o deformidades dentro de la cavidad, con apertura oral de 0 mm; en el estudio tomográfico y de reconstrucción se identifica imagen hiperdensa bien definida compatible con calcificación a nivel del músculo pterigoideo medial. Se llevó a resección de lesión, confirmándose diagnostico por histopatología. Paciente actualmente en vigilancia clínica y apoyo con fisioterapia, sin datos de recurrencia.
Comparación del tamaño transversal de incisivos laterales maxilares sin microdoncia y con microdoncia en las maloclusiones
Authors: Acosta-Pelayo Alejandra Olivia , Gutiérrez-Rojo Jaime Fabián
Published: August 2020

Objetivo. Determinar si existe una diferencia entre los tamaños transversales de los incisivos laterales maxilares en las diferentes maloclusiones de Angle y determinar la prevalencia de la microdoncia de incisivos laterales maxilares en las diferentes clases de Angle. Métodos. La investigación es de tipo descriptiva, transversal y observacional. El universo de estudio fueron 780 modelos de estudio pretratamiento de ortodoncia. La muestra fue de 190 modelos de estudio. Para determinar la presencia de microdoncia se utilizó el método de Binder y Cohen. Resultados. En la maloclusión de Clase I se encontró una frecuencia de microdoncia del 32%, en la maloclusión Clase II fue del 35% y en la maloclusión Clase III del 26%. Los incisivos laterales superiores con microdoncia y sin microdoncia tienen una media estadísticamente diferente entre ellos a un nivel de confianza mayor al 95%, esta diferencia se observa homogénea en cada una de las maloclusiones. Conclusiones. No se observaron diferencias estadísticamente significativas en el tamaño mesiodistal de los insicivos laterales maxilares al compararse entre las tres maloclusiones descritas por Angle. Se obtuvo mayor porcentaje de microdoncia en la maloclusión Clase II, y un menor porcentaje en la Clase III.
Salud bucal costarricense: análisis de la situación de los últimos años
Authors: López Torres Ana Cristina , Bermúdez Mora Gustavo Adolfo
Published: August 2020

Costa Rica ha presentado un progreso en los indicadores de salud bucodental en los últimos 10 años, esto gracias a la estructura de su Sistema Nacional de Salud. Los servicios de atención de la salud están divididos en servicio público, privado y otro mixto. La Caja Costarricense de Seguro Social es la entidad que domina el sector público y es el principal, dividido en tres niveles de atención escalonados. Este artículo busca exponer la estructura de las instituciones que conforman la vigilancia y atención de las enfermedades bucodentales más prevalentes en la población costarricense. De la misma manera, presenta el progreso de dichas condiciones a través de los últimos años. Se concluye que, a pesar del progreso obtenido, es necesario el fortalecimiento interinstitucional para afrontar los desafíos en la situación de salud bucodental actual.
Caracterización de la microbiota intestinal en robalo (Centropomus sp.) y aislamiento de bacterias probióticas potenciales
Authors: Zatán Valdiviezo Adrian Edú , Castillo Chunga Deysy , Castañeda Vargas Arnaldo Edward , Feria Zevallos Manuel Alberto , Toledo Valdiviezo Odalis Epifanía , Aguilar Zavaleta Jorge Luis , Cueva Távara Mario David , Motte Emmerik
Published: August 2020

El objetivo del estudio fue caracterizar la microbiota del tracto intestinal de robalo (Centropomus sp), proveniente de medio natural y cautiverio, además de aislar e identificar bacterias con potencial probiótico. La microbiota intestinal se caracterizó mediante metagenómica dirigida al gen 16S ARNr. Además, se sembraron muestras de mucus intestinal en medio de cultivo MRS para el aislamiento bacteriano. La identificación molecular se hizo mediante la secuenciación del gen 16S ARNr. Las capacidades probióticas se evidenciaron mediante ensayos proteolíticos y de antagonismo bacteriano frente a Plesiomona shigelloides, Aeromonas hydrophila, Aeromonas veronii y Vibrio harveyi. Los géneros bacterianos con mayor abundancia fueron Clostridium, Photobacterium, Cetobacterium y Rubritepida. Las especies bacterianas, incluyendo Klebsiella sp, dos cepas de Weisiella cibaria y Lactococcus sp fueron aisladas en agar MRS a partir del tracto intestinal. Las cepas W. cibaria mostraron amplio espectro antagonista y actividad proteolítica positiva.
Valoración de las relaciones de Rickettsemia de Anaplasma marginale con factores agroecológicos y productivos en bovinos doble propósito en sabanas inundables del Arauca, Colombia
Authors: Salamanca Carreño Arcesio , Tamasaukas Rita , Giraldo-Forero Julio Cesar , Quintero Alex Darío , Esney Hernandez Milena
Published: August 2020

El objetivo del estudio fue determinar la relación existente entre la rickettsemia de A. marginale y las interacciones agroambientales y genéticas en bovinos de doble propósito en Arauca, Colombia. Se tomaron muestra de sangre a 269 hembras de 14 fincas, tanto en la época seca como de lluvias y fueron analizadas con las técnicas de Wright y Hemacolor®. Se evaluó la interacción entre la presencia de A. marginale con las variables grupo racial, época del año, edad y número de partos, mediante el análisis de varianza. Se encontró 66.6% de animales positivos en la época seca y 100% en la época lluviosa para A. marginale (p<0.001), presentando valores de rickettsemia entre 0 y 0.14% (promedio: 0.04%). La rickettsia A. marginale presentó interacción con el grupo racial (p<0.05), siendo más resistentes los animales criollos y cruces de Pardo Suizo x cebú y de Simmental x cebú y susceptibles los animales con predominio Bos taurus. La edad, numero de parto, condición corporal y época no fueron dependientes para la rickettsemia.
Presencia de anticuerpos contra Leptospira spp en hogares con felinos y humanos en un barrio de Medellín, Colombia
Authors: Isaza Molina Daniela , Agudelo Héctor Bayron , Loaiza Erica Tatiana
Published: August 2020

Este estudio pretendió identificar la presencia de anticuerpos contra Leptospira spp en felinos y humanos convivientes del barrio Nueva Villa de La Iguaná, de la Comuna 7 de Medellín, Colombia, entre noviembre y diciembre de 2018. Datos históricos de la zona indican una seroprevalencia de 63.2% en humanos. Mediante un estudio transversal en el barrio, se seleccionaron 30 hogares donde convivían 30 personas y 32 felinos. Se les aplicó una encuesta y se tomaron muestras de sangre para detectar anticuerpos contra Leptospira spp mediante la prueba de microaglutinación (MAT). Los resultados de serorreacción contra Leptospira spp en felinos y humanos fueron negativos a leptospirosis, lo que sugiere que los felinos del barrio tienen un rol protector contra la Leptospira spp en sus dueños.
POTENTIAL TRACKING OF CYTOTOXIC ACTIVITIES OF MANGROVE PEREPATE (Sonneratia alba) ROOT EXTRACT AS AN ANTICANCER CANDIDATE
Authors: Latief Madyawati , Nelson Nelson , Amanda Hilda , Tarigan Indra Lasmana , Aisyah Siti
Published: August 2020

Mangrove plants are one of the most extensive biodiversity in Jambi. Besides functioning as a coastal ecosystem protector, it also has any potential of its chemical content. The study of mangrove’s bioactive compound is important to generate a new therapeutic agent. This study aims to obtain chemical compounds that have cytotoxic activity, reveal the chemical formula and molecular structure of that active compound, and determine the cytotoxic activity level of active compound from perepat (Sonneratia alba) root extract. The extract was isolated and purified using Liquid Vacuum Chromatography, Graphitation Column Chromatography, and Thin Layer Chromatography. The isolate was characterized using FT-IR spectrophotometry. Our result found that it corresponds to the β-sitosterol compound group with a high similarly chromophore. Furthermore, both of the extract and isolate was tested for toxicity activity using the Brine Shrimp Lethality Test (BSLT) method using Artemia salina shrimp larvae. Herein, we reported that acetone isolated extract possesses lower cell death average 100% (start 200 μg/ml) than isolate extract 80% (50μg/ml). Furthermore the toxicity of total extract has LC50 23,98 (μg/ml), while Isolate 10,04 (μg/ml). From the LC50 value, it can be concluded that the toxicity of the extract lower than the isolate, and both were classified as very toxic compounds, and very potential to be developed into anti-cancer drug compounds.
Ethanolic Extract of Xestospongia Sp. Induces CD4+ and CD14 Cells Levels on Wistar Male Rat Infected with Staphylococcus aureus
Authors: Fristiohady Adryan , Wahyuni Wahyuni , Malaka Muhammad H , Madu Dewiyanti , Muthalib Dayatriana , Munasari Dian , Purnama La Ode MJ , Sadarun Baru , Ilyas Muhammad , Sahidin Idin
Published: August 2020

Immunomodulator is a substance that increases or suppresses the immune response through the certain mechanism. The marine sponge Xestospongia sp. has immunomodulatory activity by increasing phagocytic activity. In addition, the phagocytic activity is affected by CD4+ and CD14 cells levels. Thus, this study aims to investigate the effect of Xestopongia sp. extract toward CD4+ and CD14 cells level in model rat. Animals were divided into 4 groups (n=5) and treated for 7 days, as follow: Group I (Ethanolic extract of Xestospongia sp. dose of 300 mg/KgBW); Group II (Ethanolic extract of Xestospongia sp. dose of 400 mg/KgBW); Group III (Phylantus niruri extract); and Group IV (0.5% Na CMC). On day 8, animals were infected with Staphylococcus aureus intraperitoneally. And the blood was collected by cardiac puncture and assayed with ELISA kit CD4 (elabscience®) and ELISA kit CD14 (elabscience®). Ethanolic extract of Xestospongia sp. provided high levels of both CD4+ and CD14 cells (Group II) compared to baseline (Group IV)  (p<0.05). Group I provided similar activity to group III (p>0.05) and Group II provided significant activity with higher levels of CD4+ and CD14 cells compared to group III (p<0.05). In conclusion, both doses of Xestospongia sp. extract provide activity as immunomodulator by increasing CD4+ and CD14 cells levels, yet dose of 400 mg/KgBw provides the higher immunomodulatory activity.