bluuebunny/crossref_metadata_2025 huggingface dataset:
A identidade dialetal do “manezinho” com foco em características entonacionais
Authors: Seara Izabel Christine , Sosa Juan Manuel
Published: June 2017

O falar “manezinho” é um dialeto não urbano do português brasileiro falado na Ilha de Santa Catarina que apresenta certas propriedades segmentais e suprassegmentais que o definem. Neste artigo, investigamos uma dessas propriedades suprassegmentais caracterizada pelo uso de um contorno declarativo particular, que exibe uma acentuada subida tonal até a sílaba tônica final, seguida por uma queda do tom alto para a parte inferior do registro do falante. Usamos dados provenientes do Projeto VARSUL como corpora de pesquisa, analisando esses dados a partir de uma metodologia da fonética experimental. Por meio de dois experimentos de percepção, mostramos que o contorno em questão, em notação autossegmental métrica (LH) ¡HL%, é reconhecido por um número muito significativo de ouvintes como sendo efetivamente o falar “manezinho”. As implicações para a sociofonética são os detalhes fonéticos quantitativos da queda tonal final, que dão a este contorno sua singularidade frente a outros dialetos.********************************************************************The "Manezinho" dialectal identity and its intonational featuresAbstract: "Manezinho" is a non-urban dialect of Brazilian Portuguese spoken in the Island of Santa Catarina, which has certain defining segmental and suprasegmental properties. This paper investigates one of these suprasegmental properties characterized by the use of a particular declarative contour, which shows a sharp tonal rise up to the final stressed syllable, followed by a drop from the high tone to the lower part of the speaker's range. We used datafrom the VARSUL Project as research corpus, analyzing these data with the methodology of experimental phonetics. By means of two perception tests, we show that the contour in question, in autosegmental-metrical notation (LH) ¡HL%, is recognized by a very significant number of listeners as being effectively “Manezinho”. The implications for sociophonetics are the quantitative phonetic details of the final tonal fall, which give this contour its uniquenessover other dialects.Keywords: Intonation; Brazilian Portuguese; “Manezinho”dialect; Sociophonetics
Apresentação
Authors: Brescancini Cláudia Regina , Foulkes Paul
Published: June 2017

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New distribution records of Catasetum confusum G.A. Romero-González (Cymbidieae, Epidendroideae, Orchidaceae) from Brazil
Authors: Petini-Benelli Adarilda , Smidt Eric De Camargo
Published: June 2017

Until now, Catasetum confusum G.A. Romero-González was known from the states of Goiás, Mato Grosso and Tocantins. The present study extends the distribution of this species to the states of Bahia and Piauí, as well as the Federal District, based on herbarium specimens deposited at CEN, RB, UFMT and UPCB. Following IUCN criteria, C. confusum is Endangered due to its area of occupancy, geographic distribution and habitat quality, showing the importance of these new records.
Vad mandatperioden gör med fullmäktigeledamoten: Stärks politiskt självförtroende eller blir man desillusionerad?
Authors: Erlingsson Gissur Ó , Öhrvall Richard
Published: June 2017

Abstrakt saknas
PRE-CLINICAL SKILLS: A competency-based assessment integrated course implemented early in the curriculum to prepare second-year medical students prior to entering clinical settings
Authors: Fenoll-Brunet Maria Rosa , Piera Verónica , Sánchez Domènec , Lanuza Maria Angel , García Neus , Cobos Pedro
Published: June 2017

This article was migrated. The article was marked as recommended. Objective: This Case Study describes the experience of Rovira i Virgili University School of Medicine (URV) with the early introduction of pre-clinical skills learning in the undergraduate medical curricula to monitor and assessing these competencies as a prerequisite for medical students accessing their training in clinical settings. Course Development: A PRE-CLINICAL SKILLS course has been developed to guarantee medical student's performance in managing clinically relevant basic medical sciences to interpret clinical scenarios, to develop technical communication skills and to value professional behavior throughout the first two years of medical education. The set of pre-clinical competencies evaluated in this course as well as the corresponding assessment methods have been established according to an international reference standards review work in collaboration with the regional quality assurance agency. An integrated formative assessment is being used. Course Advantages, Added Values and Outcome Measures: Since the academic year 2009-2010 about 130 students from second-year of Medicine follows this integrated trunk-course while being enrolled in parallel in other core courses as Physiology, Anatomy, and Histology. The program doesn't include lectures but only learning activities to train and monitor the successful achievement of the pre-clinical skills by medical students. A good majority of the participants achieve readiness for start training in clinical settings. As a whole, this course is useful ensuring patient's safety by identifying weaknesses acquisition of pre-clinical skills and predicting medical students who will have difficulty during their clinical training. Conclusions: Reflecting on our experience, we believe that the named course "PRE-CLINICAL SKILLS" overcomes the disadvantages of the traditional teaching methods. Helping students to conceptualize rather than memorize and encouraging them to integrate clinically relevant basic medical sciences concepts and principles by training pre-clinical skills in this competence-based assessment course prior entering into clinical settings.
An Embarrassment of Riches or a Profusion of Confusion An Evaluation of the Continued Existence of the Civil Union Act of 2006 in the Light of Prospective Domestic Partnerships Legislation in South Africa
Authors: Smith Bradley Shaun , Robinson JA Robbie
Published: June 2017

As it stands, South African family law currently holds that the Marriage Act 25 of 1961 applies exclusively to the solemnisation of heterosexual civil marriages while same-sex couples have no choice but to formalise their relationships in terms of the Civil Union Act 17 of 2006.  In addition, the legal position is complicated by the fact that the latter Act not only allows both heterosexual and homosexual couples to conclude a civil union, but also provides that a civil union may take the form of either a marriage or a civil partnership, both of which enjoy the same legal recognition as, and give rise to the same legal consequences, as a civil marriage under the Marriage Act. In January 2008, a draft Domestic Partnerships Bill saw the light of day, the potential enactment of which casts significant doubt as to whether the prevailing framework should be retained.  With this potential development in mind, this paper considers the desirability of maintaining the "separate but equal" status quo by: (a) comparing the South African Law Reform Commission's pre-Civil Union Act proposals with the approach eventually adopted by the legislature; (b) comparing and contrasting the post-Civil Union Act position in South Africa with that of an established and well-ordered jurisdiction such as the Netherlands and, in the light hereof, considering the cases for and against repealing the Civil Union Act; and (c) by considering the desirability and practicality of the civil partnership's potential co-existence with the Domestic Partnerships Bill (as modified in accordance with a recent study).  A proposal is made that could provide a less complex and better streamlined family law dispensation in South Africa.
The Impact of HIV/AIDS Regarding Informal Social Security: Issues and Perspectives from a South African Context
Authors: Tshoose Clarence Itumeleng
Published: June 2017

The purpose of the article is to examine the right to social assistance for households living with HIV/AIDS in South Africa. In particular, the article focuses on the impact of this pandemic on households' access to social assistance benefits in the wake of the HIV/AIDS pandemic, which has wrought untold sorrow and suffering to the overwhelming majority of households in South Africa.

 

The article analyses the consequences of HIV/AIDS in relation to households' support systems, care and dependency burdens, and the extent to which the household members either acknowledge the illness (enabling them to better engage with treatment options) or alternatively, deny its existence. The article commences by reviewing the literature concerning the effects and social impact of HIV/AIDS on the livelihoods of households and their families. The social reciprocity that underpins households' livelihoods is briefly recapitulated. The article concludes that, while recent policy developments are to be welcomed, the current South African legal system of social security does not provide adequate cover for both people living with HIV/AIDS and their families. More remains to be done in order to provide a more comprehensive social security system for the excluded and marginalised people who are living with HIV/AIDS and their families.
Editorial
Authors: Venter Francois
Published: June 2017

In this extraordinarily voluminous edition we offer 2 orationes, 8 articles and 3 notes.

Oratio •  The first oratio is by Josef GA Krger, QC. Partner in Borden Ladner Gervais LLP, Calgary, Canada, who spoke in August 2010 in Potchefstroom, giving an expert exposition of the Canadian law on the restructuring of insolvent corporations in Canada. •  The second oratio is by Werner Menski of the School of Law, University of London, speaking in June this year at the University of London in a Religare Conference. Showing his distaste for fuzzy law, he argues that "moderate secularism" is not merely another fuzzy concept, but it is "super-fuzzy", and that lawyers claiming to love certainty "have a tendency to sit in judgment over matters and even pre-judge things they know little about, including legal pluralism" leading to much irritation.
Property Rights And Traditional Knowledge
Authors: Cross John T
Published: June 2017

For the past several decades, there has been a push to provide some sort of right akin to an intellectual property right in traditional knowledge and traditional cultural expression. This push has encountered staunch resistance from a number of different quarters. Many of the objections are practical. However, underlying these practical concerns is a core philosophical concern. A system of traditional knowledge rights, this argument suggests, simply does not satisfy the basic rationale for granting property rights in intangibles like inventions and expressive works. Intellectual property is meant to encourage innovation and creative activity. Most traditional knowledge, by contrast, is not innovative, at least in the same sense as the inventions and works that qualify for patents and copyrights. At present, the "anti-property" camp seems to have the better of the argument, as even the World Intellectual Property Organisation has abandoned the notion of true property rights.This article seeks to refute this philosophical objection to a property model for traditional knowledge. It argues that the classic philosophical argument justifying intellectual "property" namely, that property rights are justified only as a way to spur innovation and other creative activity is incorrect in two ways. First, the argument misstates the main goal of an intellectual property system. While intellectual property may serve as an incentive for innovation, society's primary concern is not the innovation per se, but instead the dissemination of knowledge. Second, there may be policy reasons other than the development of knowledge that can justify intellectual property-like rights.The article then applies these observations to the particular question of traditional knowledge and cultural expression. It demonstrates that a system of property rights could be useful in helping to encourage the dissemination of traditional knowledge, even if that knowledge is not "new" in the classic sense. Second, other important social concerns, especially the goal of ensuring accuracy in knowledge, may justify a system of property rights. While these arguments may not ultimately support a property rights system—after all, the practical concerns remain very real—they do help to refute the underlying philosophical objection.
When Certainty and Legality Collide: The Efficacy of Interdictory Relief for the Cessation of Building Works pending Review Proceedings
Authors: Summers Richard
Published: June 2017

Effective legal redress against unlawful building works or construction activities can be an elusive target.  Given the desirability of legal certainty attached to administrative decisions in terms of which building plans are approved, should the practical implications of this principle trump the equally important principle of legality?  This article examines the – at times – competing imperatives of certainty and legality in the context of several recent decisions of the Western Cape High Court that related to applications for interdictory relief for the cessation of allegedly unlawful building works.  The practical difficulties for an applicant in these circumstances are particularly acute when the relief is sought pending the final determination of an application for judicial review of the impugned administrative decision to grant building plan approval.  The article highlights the approach of the Western Cape High Court in three cases to invoking considerations of legality in circumstances where building works had reached an advanced stage and the respondent had effectively achieved what has been described as an "impregnable position".  The principal difficulty for an applicant lies in the fact that where interdictory relief is sought against building works that have reached an advanced stage, this potentially renders an eventual successful review application brutum fulmen.
Treatments for the Preservation of Seawater Samples v1
Authors: Poulos Bonnie , Jenn Brum not provided , Elke Allers not provided , Christine Schirmer not provided
Published: June 2017

This protocol consists of several methods for the preservation of seawater samples to later perform various techniques in the lab. It is meant as a sampling treatment in the field and is written as comprehensive; however, depending on needs and scientific questions, any or all of the methods may be used. Flow Cytometry (FCM): Samples are used for counting bacteria and for sorting different groups of microbes (eg, sorting synechococcus and prochlorococcus and non-fluorescent bacteria). Glutaraldehyde fixes microbes and renders them unculturable whereas microbes preserved in DMSO and Betaine can usually be cultured after thawing and rinsing. Betaine was developed for single cell sorting and may be a better preservative than DMSO. SYBR staining: Samples are used for counting bacteria (unfiltered or pre-filtered seawater) and viruses (unfiltered, pre-filtered or 0.2um filtered seawater) microscopically after staining with SYBR-Gold (or SYBR-Green). Transmission Electron Microscopy (TEM): Samples are used for determining frequency of visibly infected cells (FVIC) and determining types of microbes or viruses present, and can be quantitative. Quantitative PCR (qPCR): Samples are used for quantitative PCR of microbes. They can also be used for quantitative PCR of viruses. Viral qPCR: Samples are used for quantitative PCR of viruses. Culturing Viruses: Samples are used for cultivating viruses by solid or liquid plaque assay on susceptible hosts. Samples can also be used for viral tagging (using flow cytometry) and most probable number (MPN) assays. Fluorescence in situ hybridization (FISH): Samples are used with fluorescent probes to determine what populations of bacteria or other microbes are present. The filters may also be used to simply count the number of microbes in a sample. For detailed FISH procedure see here.
Cesium Chloride Protocol for Phage v1
Authors: Rohwer Forest
Published: June 2017

This protocol contains 4 sections: 1. Cesium Chloride Gradient to separate phage from bacteria. Marine viruses around 1.5g/ml2. DNase I treatment of CsCl-purified phage3. Formamide Extraction of DNA from phagE4. 4. CTAB
TE Buffer v1
Authors: Alex Aitken Ms
Published: June 2017

TE is a commonly used buffer solution in molecular biology, especially in procedures involving DNA or RNA. "TE" is derived from its components: Tris, a common pH buffer, and EDTA, a molecule that chelates cations like Mg2+. The purpose of TE buffer is to solubilize DNA or RNA, while protecting it from degradation: 10 mM Tris, bring to pH 8.0 with HCl, 1 mM EDTA
Cyanobacteria Trace Metal Mixture (CTMM) v1
Authors: Sullivan Lab Matthew
Published: June 2017

Preparation of the trace metal mixture for addition to seawater for the cultivation of marine cyanobacteria, Prochlorococcus and Synechococcus
Dissecting and Immunostaining Drosophila melanogaster Egg Chambers v1
Authors: Ward's Lab Rob
Published: June 2017

Drosophila melanogaster females' productive system has been an excellent model in studying many developmental events such as stem cell specification and cell migration. The ovaries are composed of a chain of egg chambers starting with the germaruim that buds of stage 1 egg chamber after encapsulated it with follicle cells. Then, stage 1 egg chamber goes under 14 developmental stages. This protocol provides the basic method for both dissecting and immunostaining ovaries. Each step is critical. This protocol is modified from http://www.ncbi.nlm.nih.gov/pubmed/8162854
Preparation and application of fluorescently labeled virus particles v1
Authors: M. Comeau and Rachel T. Noble André
Published: June 2017

Comeau, A. M., and R. T. Noble. 2010. Preparation and application of fluorescently labeled virus particles, p. 19–29. In S. W. Wilhelm, M. G. Weinbauer, and C. A. Suttle [eds.], Manual of Aquatic Viral Ecology. ASLO. Please see the published manuscript for additional information.
Finding the core vs flexible microbiome v1
Authors: Hurwitz Bonnie
Published: June 2017

In a study comparing obese versus lean twins, Turnbaugh and colleagues found that there were "shared microbial genes among sampled individuals, comprising an extensive, identifiable 'core microbiome' at the gene, rather than at the organismal lineage level". This means that despite having differences in bacterial composition, the "core" functional repertoire of microbiome remains the samein obese versus lean twins. In this tutorial, we are going to explore these functional differences in two lean twins (TS1 and TS2) and two obese twins (TS19 and TS20) from this study. Turnbaugh, Peter J., et al. "A core gut microbiome in obese and lean twins."nature 457.7228 (2009): 480-484.
EDTA disodium salt dihydrate v1
Authors: Thornton James
Published: June 2017

For use in DNase I Treatment protocol for DNase inactivation.
Immunoprecipitation v1
Authors: Liu Vivian
Published: June 2017

This protocol is suitable for both endogeneous and overexpressed protein immunoprecipitations.
SPOTDNAExtractionfrom142mm Durapore0.22µm Filters v1
Authors: not provided RohanSachdeva , not provided CherylChow
Published: June 2017

Reference: Fuhrman et al, 1988. Extraction from Natural Planktonic Microorganisms of DNA Suitable for Molecular Biological Studies. AEM 54(6), 1426‐1429
Enumeration of virus particles in aquatic or sediment samples by epifluorescence microscopy v1
Authors: A. Suttle and Jed A. Fuhrman Curtis
Published: June 2017

This is a protocol from: Suttle, C. A. and J. A. Fuhrman. 2010. Chapter 15: Enumeration of virus particles in aquatic or sediment samples by epifluorescence microscopy. Manual of Aquatic Viral Ecology. Waco, TX:American Society of Limnology and Oceanography. doi:10.4319/mave.2010.978-0-9845591-0-7 Please see the published manuscript for additional information.
Lysate titer v1
Authors: Middelboe Mathias , Amy M. Chan not provided , and Sif K. Bertelsen not provided
Published: June 2017

For use in "Obtaining pure cyanophage stocks (liquid assay)"
HERP: Haploid Engineering and Replacement Protocol for Saccharomyces v1
Authors: G. Alexander William , Drew T. Doering not provided , and Chris Todd Hittinger not provided
Published: June 2017

This protocol is from: Alexander WG, Doering DT,andHittinger CT (2014) High-Efficiency Genome Editing and Allele Replacement in Prototrophic and Wild Strains of Saccharomyces.Genetics198:859-866;doi:10.1534/genetics.114.170118 Please see the full manuscript for additional details. The purpose of this document is to provide you with an easy‐to‐follow guide to using the HERP cassettes. We will go through the preparation of the selection and counterselection media, the culturing and transformation for insertion of the HERP cassettes, and counterseletive replacement of the HERP cassettes.
U6-based sgRNA ligation mixture v1
Authors: Yin Linlin , Lisette A. Maddison not provided , Mingyu Li not provided , Nergis Kara not provided , Matthew C. LaFave not provided , Gaurav K. Varshney not provided , Shawn M. Burgess not provided , James G. Patton not provided , and Wenbiao Chen not provided
Published: June 2017

1 µl 10x CutSmart buffer, 1 µl T4 DNA ligase buffer, 0.25µl U6 plasmid (about 100ng), 1µl annealed oligos, 0.3 µl T4 DNA ligase, 0.3 µl BsmBI, 0.2µl PstI (optional), 0.2 µl SalI (optional), 5µl H2O
Glass Bead Transformation of Chlamydomonas v1
Authors: Timmons Josh
Published: June 2017

A short and relatively cheap method for non-homologous nuclear transformation of Chlamydomonas reinhardtii. Works best with linearized DNA. Requires 500 micron glass beads for DNA transformation.
TAP Agar Plates v1
Authors: Timmons Josh
Published: June 2017

Tris-Acetate-Phosphate medium (TAP) is among the most common for Chlamydomonas reinhardtii. This protocol uses premade TAP from ThermoFisher. Alternative and significantly cheaper TAP medium can be found at PhytoTechnology. Agar is from Acros.
Preparation of Custom Synthesized RNA Transcript Standard v1
Authors: M. Satinsky Brandon , Scott M. Gifford not provided , Byron C. Crump not provided , Christina Smith not provided , Mary Ann Moran not provided
Published: June 2017

This protocol is from: Satinsky, Brandon M., et al. "Use of internal standards for quantitative metatranscriptome and metagenome analysis." Methods in enzymology 531 (2012): 237-250. Please see the full chapter for additional details.
Precision Count Beads™ Protocol and Applications v1
Authors: Miller Kelsey
Published: June 2017

Precision Count Beads™ are designed for counting the absolute number of cells in a complex mix population and other particles by flow cytometry. Precision Count Beads™ are excited by a variety of lasers including violet (405nm), blue (488nm), yellow/green (562nm), and red (633nm).
MojoSort™ Human CD4 Nanobeads Regular Protocol v1
Authors: Miller Kelsey
Published: June 2017

This procedure is optimized for the isolation of 107 to 2 x 108 cells per tube from human peripheral blood mononuclear cells (PBMCs). If working with fewer cells keep volumes as indicated for 107 cells. For best results, optimize the conditions to your specific cell number and tissue. Prepare fresh MojoSort™ Buffer solution by diluting the 5X concentrate with sterile distilled water. To maximize the purity of CD4+ T cells we recommend the depletion of monocytes, as human monocytes express CD4. To deplete monocytes from human PBMCs, we recommend the use of MojoSort™ Human CD14 Selection Kit (Cat. No. 480025/480026). Alternatively, you may consider the Human CD4 T Cell Selection Kit (Cat. No. 480038). After monocyte depletion proceed to isolate the CD4+ T cells. You can also use other methods to deplete monocytes. If you wish to include monocytes in your CD4+ cell isolation, depletion is not required.
MojoSort™ Negative Selection Columns Protocol v1
Authors: Miller Kelsey
Published: June 2017

BioLegend MojoSort™ nanobeads work in commonly used separation columns, based on our internal research as well as validation by external testing by academic labs. This simple protocol consists of following the MojoSort™ protocol to label the cells with pre-diluted MojoSort™ reagents and using the columns as indicated by the manufacturer Note: Due to the properties of our beads, it may be possible to use far fewer beads and less antibody cocktail that with other commercial suppliers. We recommend a titration to find the best dilution factor. However, as a general rule, dilutions ranging from 1:2 to 1:10 for the antibody cocktail can be used. Dilutions ranging from 1:5 to 1:20 for the Streptavidin Nanobeads can be used.
Swift™ Membrane Stain v1
Authors: not provided G-Biosciences
Published: June 2017

Rapid, Sensitive and Reversible Protein Stain For PVDF and Nitrocellulose Membranes. (These instructions are for a single 8 x 8 cm membrane.Increase the reagent volumes with larger membranes.)
SpinOUT™: Protein Desalting v1
Authors: not provided G-Biosciences
Published: June 2017

SpinOUT™ protocol for desalting of peptide and protein samples. (Cat. # 786-170 to 786-173, 786-703 to 786-708, 786-865 to 786-869)
MegaLong™ Protocol for Isolation of >100kb Genomic DNA (Tissue Sample) v1
Authors: not provided G-Biosciences
Published: June 2017

MegaLong™ isolates high molecular weight (>100kb) genomic DNA from a variety of samples, including animal tissues, cultured cells, whole blood, bacterial and yeast. The protocol described here is for tissue samples. Please refer to the appropriate protocol, depending on your application.
XIT™ Genomic DNA Blood Kit Protocol for Isolation of Genomic DNA (for 0.5ml blood) v1
Authors: not provided G-Biosciences
Published: June 2017

This is a protocol forthe Isolation of Genomic DNA from Fresh Blood, Bone Marrow & Buffy Coat (0.5ml blood). (Cat. # 786‐294, 786‐295, 786‐296)
VBRC Base By Base: Import genes from Genbank file v1
Authors: Tang Nick
Published: June 2017

Viral Bioinformatic Resource Centre Provide databases of viral genomic information. Please check theOrganismsmenu to see which viruses we support: we’re now focusing on large DNA viruses The VOCs (Virus Orthologous Clusters) database is at the heart of our system. The database links directly to integrated tools for comparative analyses. VOCs sorts genes into ortholog clusters (e.g. RNA polymerase) to simplify data retrieval. Provide easy access to the genes, gene families, and genomes of the different virus families. via a unique series of powerful Java tools that support multiple computer platforms (seeVBRC Tools menu). design and build software to tackle specific bioinformatics/virology problems, often in collaboration with virologists. Rally the research community to provide expert curation of these viral genomes by: Adding value to GenBank sequences through enhancing and updating genome annotations Linking to research reviews/papers for the research community. Collaborate with researchers to help on specific bioinformatics problems, e.g. Custom searches of the databases Building new features into our tools Help with genome annotation
Script P9: Phage-Bacteria Interaction Network v1
Authors: GD HANNIGAN , GRICE EA not provided , ET AL. not provided
Published: June 2017

This protocol provides a method to study phage-bacteria interactions by generating a Network in Cytoscape using the application CoNet. To CoNet tutorial can help with analysis.Based on the methods from the following publication: Hannigan, Geoffrey D., et al. "The Human Skin Double-Stranded DNA Virome: Topographical and Temporal Diversity, Genetic Enrichment, and Dynamic Associations with the Host Microbiome."mBio6.5 (2015): e01578-15.
Script R1: Virome Contig and Sequencing Statistics v1
Authors: GD HANNIGAN , GRICE EA not provided , ET AL. not provided
Published: June 2017

This protocoloutlines the analysis used to plot contig coverage statistics, as well as sequence count and length stats. We begin with visualizing contig length vs coverage. We then visualize the distributions of sequence counts per sample as a probability density plot (similar idea as a histogram), and then do the same for median sequence length. Based on the methods from the following publication: Hannigan, Geoffrey D., et al. "The Human Skin Double-Stranded DNA Virome: Topographical and Temporal Diversity, Genetic Enrichment, and Dynamic Associations with the Host Microbiome."mBio6.5 (2015): e01578-15.
Stellaris® RNA FISH Simultaneous IF + FISH in Adherent Cells Protocol v1
Authors: Biosearch Technologies LGC
Published: June 2017

Stellaris RNA FISH protocol to simultaneously label adherent cells with IF and RNA FISH.
CviJI Assay Conditions v1
Authors: DUNIGAN AND IRINA AGARKOVA DAVID
Published: June 2017

For use in CviJI Purification From IL-3A Virus Infected NC64A Chlorella.
Clean-up using AMPure XP beads v1
Authors: not provided Bioline
Published: June 2017

This is the suggested protocol for clean-up using AMPure XP beads. It is recommended as part of theJetSeqTM DNA Library Preparation Kit protocol for post ligation, post adaptor extenstion (PCR1), and post indexing clean-ups.
DNase I Treatment of Purified RNA in Solution for ISOLATE II Biofluids RNA Kit v1
Authors: not provided Bioline
Published: June 2017

The ISOLATE II Biofluids RNA Kit isolates total RNA with minimal amounts of genomic DNA contamination using the supplied Genomic DNA Removal Column. However, additional DNase I treatment may be required in certain cases e.g. the amount of genomic DNA in the sample exceeds the capacity of the Genomic DNA Removal Column, or performing a highly sensitive application. The on-column DNase I digestion results in minimal residual DNA, undetectable in most downstream applications. For the most sensitive applications, DNA digestion in solution is recommended to eliminate even traces of contaminating DNA. Stringent RNase control is needed as well as RNA repurification to remove buffer, salts, DNase I and digested DNA.
Time Efficient Method for Washing 15 mL Vials v1
Authors: John Seth
Published: June 2017

This protocol is designed to allow you to clean large numbers of 15 mL vials to the highest standards of cleanliness, but in a time-efficient manner.
C_HW2: Makefile, command line exercises with yeast v5
Authors: Youens-Clark Ken
Published: June 2017

Yeast is a well-characterized genome due to its small size and historical significance in genetics. The websitehttp://yeastgenome.org/ is a dedicated resource for yeast genomics. For this exercise, I want you to create a "Makefile" that will execute this entire pipeline if I type "make."
C_HW2: Makefile, command line exercises with yeast v6
Authors: Youens-Clark Ken
Published: June 2017

Yeast is a well-characterized genome due to its small size and historical significance in genetics. The websitehttp://yeastgenome.org/ is a dedicated resource for yeast genomics. For this exercise, I want you to create a "Makefile" that will execute this entire pipeline if I type "make."
Replacing a Bitstream at the Woods Hole Open Access Server v1
Authors: Shepherd Adam
Published: June 2017

Describes how to update files at the Woods Hole Open Access Server - the institutional repository used by BCO-DMO for dataset versioning.
Análisis de un modelo conversacional para ambientes virtuales de aprendizaje
Authors: Morales Martín Luz Yolanda , Leiva-lobos Edmundo , Antillanca Espina Héctor
Published: June 2017

Este artículo analiza los límites de un modelo para facilitar el aprendizaje colaborativo en ambientes electrónicos llamado Modelo de Conversación para el Aprendizaje (MCA). Este análisis demanda cuestionar los supuestos implícitos que tienen nociones usadas por los autores del modelo tales como la conectividad comunicativa y el modelo conversacional adoptado. La propuesta termina haciendo reflexiones sobre el diálogo, proponiendo líneas concretas para reparar el MCA y haciendo indicaciones sobre artefactos más concretos que se deben incluir en una nueva versión del modelo.
Evaluación de aditivos usados en el tratamiento de arcillas expansivas
Authors: Camacho Tauta Javier Fernando , Reyes Ortiz Oscar Javier , Mayorga Antolínez Catalina , Méndez G. Dolly Fernanda
Published: June 2017

Se utilizaron tres aditivos (cal, cenizas volantes y aceite sulfonado) para disminuir las propiedades de una arcilla altamente expansiva (bentonita), con el fin de determinar las ventajas técnicas de cada alternativa bajo las mismas condiciones y ensayo. Para tal fin la bentonita se mezcló con diferentes concentraciones de aditivo y se realizaron ensayos de caracterización física y mecánica que permitieron analizar el comportamiento de cada uno. Se encontró que las cenizas volantes requieren ser adicionadas en cantidades excesivas para lograr disminución del potencial de expansión a valores admisibles. Con la cal se encontró un valor óptimo del 10% ya que logró una reducción considerable en las propiedades expansivas de la bentonita. Los resultados obtenidos con el aceite sulfonado evidenciaron que la estabilización electroquímica que lleva a cabo, requiere de factores ambientales favorables para desarrollar su efecto.
Laboratorio virtual en la formación por competencias
Authors: Pérez Nelson , Amaya Darío , Ramos Olga
Published: June 2017

Una de las necesidades del país en la formación técnica, es la cobertura de mano de obra calificada en áreas técnicas, como la automatización de procesos industriales y, ahora con la globalización de los mercados, la industria requiere personal que apoye los procesos de modernización tecnológica que permitan implementar líneas de producción eficientes; para esto, es necesario contar con laboratorios especializados que permitan al individuo adquirir competencias en este campo. En este artículo se plantea el desarrollo de un laboratorio virtual que representa un proceso didáctico.
La producción como origen del plusvalor en la teoría marxista
Authors: Boundi Chraki Fahd
Published: June 2017

El objetivo de la presente investigación es doble. Por una parte, consiste en dilucidar que la fuente misma de la ganancia, esto es, el plusvalor, dimana de la esfera de producción. Por otra, demostrar que la condición necesaria y suficiente para el sostenimiento del orden de sociedad capitalista reside en que entre el capital y el trabajo ha de mediar una relación de dominación expresada en la explotación de la fuerza de trabajo. Para este propósito, se confronta la teoría de Marx con el enfoque marginalista. Seguidamente, se descifra el secreto del plusvalor en la compraventa de fuerza trabajo, manifestando de esta suerte el carácter dual del sistema de cálculo marxiano. Finalmente, por medio del teorema marxiano fundamental se verifica la hipótesis de que la explotación de la fuerza de trabajo es condición necesaria y suficiente para que los capitalistas obtengan ganancias positivas.
Leda Valladares, poeta: misticismo existencial en un campo literario de provincia
Authors: Orquera Fabiola
Published: June 2017

Leda Valladares (1919-2013), reconocida intérprete y recopiladora de coplas en el norte argentino, se ha destacado también como poeta y cancionista. Este corpus incluye una sección en la revista Cántico (1940), los libros Se llaman llanto o abismo (1944), Yacencia (1954), Mutapetes (1963), Camalma (1971) y el disco de canciones Solamente (1964). Tomamos las tres primeras obras para internarnos en un misticismo contemporáneo atravesado por una actitud existencialista y una subjetividad provinciana y proto-feminista, mostrando la particular asimilación de lo occidental que se da en escritores no centrales.
Adsorption/desorption and biofunctional properties of oleuropein loaded on different types of silk fibroin matrices
Authors: Bayraktar Oguz , Balta Ali Bora , Basal Bayraktar Guldemet
Published: June 2017

The objective of this study was to investigate the adsorption/desorption behavior of oleuropein on different types of silk fibroin matrices including silk fibroin microfibers (MF), regenerated silk fibroin (RSF), and silk fibroin nanofibers (NF). Nanofibers with an average diameter of ranging between 24 and 326 nm were successfully prepared using the electrospinning technique. The effects of the silk fibroin concentration, the voltage applied and the distance between needle tip and collector plate on the morphology of the NF were investigated. The adsorption capacities of MF, RSF and NF were determined as 104.92, 163.07 and 228.34 mg oleuropein per gram of material, respectively. The percentage of initially adsorbed oleuropein that was desorbed was 86.08, 91.29 and 96.67% for MF, RSF and NF, respectively.NF and RSF discs loaded with oleuropein were subjected to disc diffusion assays to determine their antibacterial activity against test microorganisms Staphylococcus epidermidis (Gram +) and Escherichia coli (Gram – ). The results showed that both biomaterials possessed antibacterial properties after loading with oleuropein. Wound scratch assays using oleuropein released from NF revealed an enhancement of cell migration, indicating a wound healing property of the material.In conclusion, the NF can be utilized as a biofunctional polymeric material with better performance for the adsorption and desorption of oleuropein compared with MF and RSF.
Augustus Welby Northmore Pugin : ideología y teoría a través de sus textos = Welby Northmore Pugin : Ideology and theory based in his texts
Authors: Dávila Romano Daniel
Published: June 2017

Augustus Welby Northmore Pugin destacó como un “ideólogo”  de la arquitectura. Sin embargo, la ideología arquitectónica de Pugin se desarrolla por medio de conceptos claros y distintos que ofrecen análisis racionales de su arquitectura   contemporánea. Son estos conceptos, apuntados en Contrasts (1836) y después desarrollados en The true principles of  pointed or Christian architecture (1841), los que se explican en este trabajo mediante la extracción y el comentario de citas del autor y de algunos de sus críticos modernos más destacados. Lo pretendido en este trabajo es un acercamiento textual aunque abreviado a las ideas acerca de cómo creía Pugin que  la arquitectura debía ser. Abstract Augustus Welby Northmore Pugin excelled as “ideologist” of  architecture. However, Pugin’s architectural theory derives from  clear and distinct concepts that offer a rational analysis of the architecture of his time. It is in fact these concepts, which appear in their first traits in Contrasts and then fully developed in The true principles of pointed or Christian architecture: set  forth in two lectures delivered at St. Marie's, Oscott (1841), what this paper has focused on, by means of analysing a series of  selected quotes. They include also several commentaries from some of his more importan critics of the recent times. As far as posible, this paper-document aims to present what we think  Pugin believed that architecture should be.
Islas de generación: el discurso visual en torno a las factorías de energía, 1940-1965
Authors: O Cabrera Manuel Rodrigo de la
Published: June 2017

El artículo profundiza en los territorios históricamente dedicados a la generación de la energía en España y más concretamente en el paisaje construido por los grandes complejos para la producción de electricidad e hidrocarburos que fueron promovidos por el sector público entre 1940 y 1965. Desarrolla una aproximación discursiva a través del análisis de fotografías y películas documentales disponibles en fondos de archivo de las empresas. Las imágenes estudiadas exponen el conflicto estético y cultural que supuso la llegada de los grandes artefactos de la energía a las áreas rurales y naturales. Ofrecen una alternativa a la percepción ordinaria, lo que obliga a lidiar mentalmente con su intensidad y poder. En consecuencia, permiten  abordar el conflicto que introduce el paisaje industrial en general, y el de la energía en particular, en la relación afectiva de los individuos con su entorno circundante.
Arquitectura telefónica clásica: el edificio industrial anuncio
Authors: García Algarra Javier
Published: June 2017

La telefonía ha sido una de los servicios urbanos que más esfuerzo ha invertido en ocultar la infraestructura necesaria para su funcionamiento. Las centrales, que son contenedores técnicos de equipos, tuvieron que instalarse en el centro de las ciudades por necesidades técnicas. Desde los inicios del negocio se procuró que los edificios se pareciesen lo más  posible alos del entorno en el que se emplazaban. Esto ha hecho que recibieran poca atención entre los investigadores de la arquitectura industrial. Externamente no parecen una factoría y el acceso a su interior, donde este carácter se revela de forma descarnada, está muy limitado al personal de las compañías propietarias. En este artículo se describe cómo se elaboraron las ideas que  guiaron el desarrollo de la arquitectura telefónica en su periodo clásico, y cómo ese modelo de origen estadounidense se implantó en España a partir de 1924.
SPIDER DIVERSITY (ARACHNIDA: ARANEAE) OF THE TEA PLANTATION AT SERANG VILLAGE, KARANGREJA SUB-DISTRICT, DISTRICT OF PURBALINGGA
Authors: Sibarani Gianti , Widhiono Imam , Darsono Darsono
Published: June 2017

Spiders are crucial in controlling insect pest population. The various cultivation managements such as fertilizer and pesticide application, weeding, pruning, harvesting, and cropping system affect their diversity. In the plantation, vegetation diversification has applied various practices, including monoculture, and intercropping, which influence the spider community. Thus, this study was intended to determine the spider abundance and diversity of the tea plantation, and the intercropping field (tea and strawberry) at Serang village, Karangreja Sub-District, District of Purbalingga. A survey and purposive sampling techniques were conducted, then the spiders were hand collected. Shannon-Wiener diversity (H’), Evenness (E), Simpson’s dominance (D), and Sorensen’s similarity (IS) indices were used to measure the spider diversity. The results revealed a total number of 575 individual spiders from 10 families, i.e., Araneae, Araneidae, Clubionidae, Linyphiidae, Lycosidae, Nephilidae, Oxyopidae, Salticidae, Tetragnathidae, Theridiidae, and Thomisidae. Araneidae was the most abundant in both fields. The total abundance of spiders in tea plantation (379 individuals), however, was greater than that in the intercropping field (196 individuals). Shannon-Wiener diversity reached H’= 1.873 in the plantation, and H’= 1.975 in the intercropping field.
Mapping Wireless Access Network Traffic to Multiservice Provisioning Platform
Authors: Ncemane Sandisiwe N. ,   South African Broadcasting Corporation, Chan H. Anthony , Karem Tope R. ,   Department of Electrical Engineering, University of Cape Town
Published: June 2017

Wireless access is needed to serve rural and urban sectors in the developing nations, while simultaneously encompassing both data and voice traffic in the same core network. We propose an architectural design of Multiservice Provisioning Platform (MSPP) for WiMAX over SONET/SDH, which involves interworking conventional technologies to deliver a solution that caters to unique situations that favor wireless access network over fully cabled systems. It consists of Wireless Access Network design, 2-way interlinking section, and the MSPP – optical core Network. It enhances the versatility of the MSPP and uses the SONET/SDH core network efficiently.[1]
Opening Address
Authors: Takama Editor: Prof. Yasufumi ,   Tokyo Metropolitan University
Published: June 2017

The JACIII was first published in 1997, and 2017 marks its 20th anniversary. During the last two decades, the research fields in computational intelligence have rapidly evolved owing to the spread of the Internet, performance improvement of computers, and accumulation of scientific knowledge. To celebrate this 20th anniversary, we have selected 6 important research areas from the JACIII scope, and invited outstanding researchers from each of these areas to contribute papers about the progress and major topics in those areas during the past 20 years. Submitted paper went through a peer-review process by distinguished professors to further improve the quality. The research areas selected were computational intelligence, fuzzy intelligence, intelligent robots, artificial intelligence and web intelligence, data mining, and smart grids. Each of those paper covers broad topics appeared in the research areas, from which readers could grasp what happened during the past 20 years. We also hope readers could find some hints about future directions of their own researches towards the next 20 years. Invited Paper 1: Computational Intelligence: Retrospection and Future Author: Witold Pedrycz (University of Alberta, Canada) Invited Paper 2: Fuzzy Inference: Its Past and Prospects Authors: Kiyohiko Uehara (Ibaraki University, Japan) and Kaoru Hirota (Beijing Institute of Technology, China) Invited Paper 3: Relationship Between Human and Robot in Nonverbal Communication Authors: Yukiko Nakagawa and Noriaki Nakagawa (RT Corporation, Japan) Invited Paper 4: Web Intelligence and Artificial Intelligence Author: Yasufumi Takama (Tokyo Metropolitan University, Japan) Invited Paper 5: A Review of Data Mining Techniques and Applications Authors: Ratchakoon Pruengkarn, Kok Wai Wong, and Chun Che Fung (Murdoch University, Australia) Invited Paper 6: Development and Current State of Smart Grids: A Review Author: Ken Nagasaka (Tokyo University of Agriculture and Technology, Japan)
Continuous transformation of chiral pharmaceuticals in enzymatic membrane bioreactors for advanced wastewater treatment
Authors: Nguyen Luong N. , Hai Faisal I. , McDonald James A. , Khan Stuart J. , Price William E. , Nghiem Long D.
Published: June 2017

This study demonstrates continuous enantiomeric inversion and further biotransformation of chiral profens including ibuprofen, naproxen and ketoprofen by an enzymatic membrane bioreactor (EMBR) dosed with laccase. The EMBR showed non-enantioselective transformations, with high and consistent transformation of both (R)- and (S)-ibuprofen (93 ± 6%, n= 10), but lower removals of both enantiomers of naproxen (46 ± 16%, n= 10) and ketoprofen (48 ± 17%, n= 10). Enantiomeric analysis revealed a bidirectional but uneven inversion of the profens, for example 14% inversion of (R)- to (S)- compared to 4% from (S)- to (R)-naproxen. With redox-mediator addition, the enzymatic chiral inversion of both (R)- and (S)-profens remained unchanged, although the overall conversion became enantioselective; except for (S)-naproxen, the addition of redox mediator promoted the degradation of (R)-profens only.
Cactus Opuntia as natural flocculant for urban wastewater treatment
Authors: Rachdi Raouen , Srarfi Feyda , Shimi Najet Slim
Published: June 2017

The cactus tree, species Opuntia ficus-indica, is a primary material of many products in various domains such as cosmetics, medicine and nutrition. In the present work, we assess its potential as a flocculant. We tried a technique which adopts three sequential treatments that used coagulation, flocculation and sedimentation processes under certain operating conditions. For this purpose, we used the aluminum sulfate (AS) as coagulant and fresh cladodes juice (FCJ) as bioflocculant. All tests were carried out on high turbid urban wastewater collected from the Metlaoui's Wastewater Treatment Plant (MWTP) (in Gafsa in southwest Tunisia). Experiments with this couple AS/FCJ show very interesting results: a high-removal of turbidity (TUR), suspended solids (SS) and chemical oxygen demand (COD). The percentages of abatement of these parameters are respectively 93.65%, 82.75% and 64.30%. The experimental results of the present study prove that the turbidity, SS and COD removal efficiency of new technique is superior to that of conventional process (with only AS). By this technique, we save 50% in AS dose. Moreover, flocs formed by the treatment using AS/FCJ are coarse and readily settleable.