bluuebunny/crossref_metadata_2025 huggingface dataset:
Privacy and Utility of Private Synthetic Data for Medical Data Analyses
Authors: Appenzeller Arno , Leitner Moritz , Philipp Patrick , Krempel Erik , Beyerer Jürgen
Published: December 2022

The increasing availability and use of sensitive personal data raises a set of issues regarding the privacy of the individuals behind the data. These concerns become even more important when health data are processed, as are considered sensitive (according to most global regulations). Privacy Enhancing Technologies (PETs) attempt to protect the privacy of individuals whilst preserving the utility of data. One of the most popular technologies recently is Differential Privacy (DP), which was used for the 2020 U.S. Census. Another trend is to combine synthetic data generators with DP to create so-called private synthetic data generators. The objective is to preserve statistical properties as accurately as possible, while the generated data should be as different as possible compared to the original data regarding private features. While these technologies seem promising, there is a gap between academic research on DP and synthetic data and the practical application and evaluation of these techniques for real-world use cases. In this paper, we evaluate three different private synthetic data generators (MWEM, DP-CTGAN, and PATE-CTGAN) on their use-case-specific privacy and utility. For the use case, continuous heart rate measurements from different individuals are analyzed. This work shows that private synthetic data generators have tremendous advantages over traditional techniques, but also require in-depth analysis depending on the use case. Furthermore, it can be seen that each technology has different strengths, so there is no clear winner. However, DP-CTGAN often performs slightly better than the other technologies, so it can be recommended for a continuous medical data use case.
An In Vitro Model Using TRIS-Buffered Plasma-Activated Water to Reduce Pathogenic Microorganisms Involved in Digital Dermatitis Infection in Cattle
Authors: Große-Peclum Vanessa , Siekmann Lisa , Krischek Carsten , Avramidis Georg , ten Bosch Lars , Harms Marcus , Ochs Christian , Ortmann Rinat , Hoedemaker Martina , Ahlfeld Birte , Roolfs Karolina Anna , Viöl Wolfgang , Plötz Madeleine
Published: December 2022

Bovine digital dermatitis is an important infectious claw disease caused by multimicrobial infections with bacteria such as Fusobacterium (F.) necrophorum or Porphyromonas (P.) levii. To analyze the antibacterial effects of a TRIS-buffered plasma-activated water (Tb-PAW) on the bacterial number of F. necrophorum, P. levii, Escherichia (E.) coli, Staphylococcus (S.) aureus and Clostridium (C.) sporogenes 1 mL of each bacterial solution (106–108 CFU/mL) was incubated with 9 mL Tb-PAW up to 15 min. E. coli, F. necrophorum and P. levii were significantly reduced by 5.0 log after 1 min of treatment, while S. aureus and C. sporogenes required 15 min to reach a 3.0 log reduction. The addition of bovine serum albumin did not negatively affect the bactericidal effect. Tb-PAW storage at 7 °C and 21 °C is possible for up to 24 h without any change in the bactericidal effect, while Tb-PAW stored at 30 °C can only be used for a period of 12 h. The present data indicate that Tb-PAW can be used to reduce various bacteria even under the influence of different parameters. However, due to the complexity of Tb-PAW, further studies are required to ensure its microbicidal activity before practical application.
Preventive and Therapeutic Effects of Punica granatum (Pomegranate) in Respiratory and Digestive Diseases: A Review
Authors: Alkhatib Mariam , Fayad Chantal , Badran Adnan , Hamade Kamar , Daou Anis , Baydoun Elias , Hijazi Akram
Published: December 2022

The pomegranate fruit is made of white to deep purple seeds that are enclosed in a white, spongy, astringent membrane, also known as pericarp, covered by a thick red skin and a crown-shaped calyx. It contains a variety of beneficial ingredients, including flavonoids, ellagitannin, punicalagin, ellagic acid, vitamins, and minerals. Pomegranates possess numerous health benefits, and their use in disease treatment has been widely recognized since antiquity. This fruit was known to exhibit several biological properties, including antibacterial, anti-inflammatory, antioxidant, and anticancer activities. Pomegranate has been used in a variety of medical systems for the treatment and therapy of a wide range of diseases and illnesses. This review summarizes studies highlighting the potential role of pomegranate in the prevention and treatment of diseases related to respiratory and digestive systems.
A Systematic Review: To Increase Transportation Infrastructure Resilience to Flooding Events
Authors: Watson Grace , Ahn Jeong Eun
Published: December 2022

This study investigated literature databases of Google Scholar and Scopus from 1900 to 2021 and reviewed relevant studies conducted to increase transportation infrastructure resilience to flood events. This review has three objectives: (1) determine which natural hazard or natural disaster had the most vulnerability studies; (2) identify which infrastructure type was most prevalent in studies related to flood resilience infrastructure; and (3) investigate the current stage of research. This review was conducted with three stages. Based on stage one, floods have been extremely present in research from 1981 to 2021. Based on stage two, transportation infrastructure was most studied in studies related to flood resilience. Based on stage three, this systematic review focused on a total of 133 peer-reviewed, journal articles written in English. In stage three, six research categories were identified: (1) flood risk analysis; (2) implementation of real-time flood forecasting and prediction; (3) investigation of flood impacts on transportation infrastructure; (4) vulnerability analysis of transportation infrastructure; (5) response and preparatory measures towards flood events; and (6) several other studies that could be related to transportation infrastructure resilience to flood events. Current stage of studies for increasing transportation resilience to flood events was investigated within these six categories. Current stage of studies shows efforts to advance modeling systems, improve data collections and analysis (e.g., real-time data collections, imagery analysis), enhance methodologies to assess vulnerabilities, and more.
Increase in the Intensity of Air–Sea Coupling in the Key ENSO Region during 1955–2020
Authors: Liu Zhiqing , Xu Jianjun
Published: December 2022

The El Niño and Southern Oscillation (ENSO), a phenomenon of air–sea coupling in the tropical Pacific, has strong response to global climate change. In this study, the primary region where ENSO occurred during the period 1955–2020 was selected as the key ENSO region, and the changes in air–sea coupling in this region were explored. The New Southern Oscillation Index (NSOI), modified from the previous Southern Oscillation Index, represents atmospheric changes, and the Niño-3.4 index represents oceanic changes. The absolute value of the running correlation coefficient between the Niño-3.4 index and NSOI in the 121-month time window was defined as the Intensity of Air–Sea Coupling (IASC) in the key ENSO region. The results showed that the IASC has significantly increased, with a confidence level of 95%, during the period 1955–2020, and the range where the correlation coefficient between the Niño-3.4 index and the sea level pressure anomaly over the key ENSO region was greater than 0.6 has evidently expanded in the context of global warming, which corresponded to the increase in IASC. Moreover, the coupling positions of sea surface temperature and wind anomalies changed, tending to the east of the equatorial Pacific during 1977–1998, and to the west during 1999–2020.
Evidence for the Magnetoionic Nature of Oblique VHF Reflections from Midlatitude Sporadic-E Layers
Authors: Deacon Chris , Mitchell Cathryn , Watson Robert , Witvliet Ben A.
Published: December 2022

Mid-latitude sporadic-E (Es) is an intermittent phenomenon of the lower E region of the ionosphere. Es clouds are thin, transient, and patchy layers of intense ionization, with ionization densities which can be much higher than in the background ionosphere. Oblique reflection of radio signals in the very high frequency (VHF) range is regularly supported, but the mechanism for it has never been clearly established—specular reflection, scattering, and magnetoionic double refraction have all been suggested. This article proposes using the polarization behaviour of signals reflected from intense midlatitude sporadic-E clouds as an indicator of the true reflection mechanism. Results are presented from a measurement campaign in the summer of 2018, which gathered a large amount of data at a receiving station in the UK using 50 MHz amateur radio beacons as signal sources. In all cases the signals received were elliptically polarized, despite being transmitted with linear polarization; there were also indications that polarization behaviour varied systematically with the orientation of the path to the geomagnetic field. This represents, for all the examples recorded, clear evidence that signals were reflected from midlatitude Es by magnetoionic double refraction.
Trust-Based Data Communication in Wireless Body Area Network for Healthcare Applications
Authors: Ramaswamy Sangeetha , Gandhi Usha Devi
Published: December 2022

A subset of Wireless Sensor Networks, Wireless Body Area Networks (WBAN) is an emerging technology. WBAN is a collection of tiny pieces of wireless body sensors with small computational capability, communicating short distances using ZigBee or Bluetooth, an application mainly in the healthcare industry like remote patient monitoring. The small piece of sensor monitors health factors like body temperature, pulse rate, ECG, heart rate, etc., and communicates to the base station or central coordinator for aggregation or data computation. The final data is communicated to remote monitoring devices through the internet or cloud service providers. The main challenge for this technology is energy consumption and secure communication within the network and the possibility of attacks executed by malicious nodes, creating problems for the network. This system proposes a suitable trust model for secure communication in WBAN based on node trust and data trust. Node trust is calculated using direct trust calculation and node behaviours. The data trust is calculated using consistent data success and data aging. The performance is compared with an existing protocol like Trust Evaluation (TE)-WBAN and Body Area Network (BAN)-Trust which is not a cryptographic technique. The protocol is lightweight and has low overhead. The performance is rated best for Throughput, Packet Delivery Ratio, and Minimum delay. With extensive simulation on-off attacks, Selfishness attacks, sleeper attacks, and Message suppression attacks were prevented.
Structural Evaluation and Conformational Dynamics of ZNF141T474I Mutation Provoking Postaxial Polydactyly Type A
Authors: Ali Yasir , Ahmad Faisal , Ullah Muhammad Farhat , Haq Noor Ul , Haq M. Inam Ul , Aziz Abdul , Zouidi Ferjeni , Khan M. Ijaz , Eldin Sayed M.
Published: December 2022

Postaxial Polydactyly (PAP) is a congenital disorder of limb abnormalities characterized by posterior extra digits. Mutations in the N-terminal region of the Zinc finger protein 141 (ZNF141) gene were recently linked with PAP type A. Zinc finger proteins exhibit similarity at their N-terminal regions due to C2-H2 type Zinc finger domains, but their functional preferences vary significantly by the binding patterns of DNA. Methods: This study delineates the pathogenic association, miss-fold aggregation, and conformational paradigm of a missense variant (c.1420C > T; p.T474I) in ZNF141 gene segregating PAP through a molecular dynamics simulations approach. Results: In ZNF141 protein, helices play a crucial role by attaching three specific target DNA base pairs. In ZNF141T474I protein, H1, H3, and H6 helices attain more flexibility by acquiring loop conformation. The outward disposition of the proximal portion of H9-helix in mutant protein occurs due to the loss of prior beta-hairpins at the C terminal region of the C2-H2 domain. The loss of hydrogen bonds and exposure of hydrophobic residues to solvent and helices turning to loops cause dysfunction of ZNF141 protein. These significant changes in the stability and conformation of the mutant protein were validated using essential dynamics and cross-correlation maps, which revealed that upon point mutation, the overall motion of the proteins and the correlation between them were completely different, resulting in Postaxial polydactyly type A. Conclusions: This study provides molecular insights into the structural association of ZNF141 protein with PAP type A. Identification of active site residues and legends offers new therapeutic targets for ZNF141 protein. Further, it reiterates the functional importance of the last residue of a protein.
From Yeast to Biotechnology
Authors: Patel Alok , Rova Ulrika , Christakopoulos Paul , Matsakas Leonidas
Published: December 2022

Yeasts are widely used in various sectors of biotechnology, from white (industrial) to red (medical) [...]
Automatic Assessment of Procedural Skills Based on the Surgical Workflow Analysis Derived from Speech and Video
Authors: Guzmán-García Carmen , Sánchez-González Patricia , Oropesa Ignacio , Gómez Enrique J.
Published: December 2022

Automatic surgical workflow analysis (SWA) plays an important role in the modelling of surgical processes. Current automatic approaches for SWA use videos (with accuracies varying from 0.8 and 0.9), but they do not incorporate speech (inherently linked to the ongoing cognitive process). The approach followed in this study uses both video and speech to classify the phases of laparoscopic cholecystectomy, based on neural networks and machine learning. The automatic application implemented in this study uses this information to calculate the total time spent in surgery, the time spent in each phase, the number of occurrences, the minimal, maximal and average time whenever there is more than one occurrence, the timeline of the surgery and the transition probability between phases. This information can be used as an assessment method for surgical procedural skills.
Structural Insights into the Dimeric Form of Bacillus subtilis RNase Y Using NMR and AlphaFold
Authors: Morellet Nelly , Hardouin Pierre , Assrir Nadine , van Heijenoort Carine , Golinelli-Pimpaneau Béatrice
Published: December 2022

RNase Y is a crucial component of genetic translation, acting as the key enzyme initiating mRNA decay in many Gram-positive bacteria. The N-terminal domain of Bacillus subtilis RNase Y (Nter-BsRNaseY) is thought to interact with various protein partners within a degradosome complex. Bioinformatics and biophysical analysis have previously shown that Nter-BsRNaseY, which is in equilibrium between a monomeric and a dimeric form, displays an elongated fold with a high content of α-helices. Using multidimensional heteronuclear NMR and AlphaFold models, here, we show that the Nter-BsRNaseY dimer is constituted of a long N-terminal parallel coiled-coil structure, linked by a turn to a C-terminal region composed of helices that display either a straight or bent conformation. The structural organization of the N-terminal domain is maintained within the AlphaFold model of the full-length RNase Y, with the turn allowing flexibility between the N- and C-terminal domains. The catalytic domain is globular, with two helices linking the KH and HD modules, followed by the C-terminal region. This latter region, with no function assigned up to now, is most likely involved in the dimerization of B. subtilis RNase Y together with the N-terminal coiled-coil structure.
Effects of Calcium Ions on the Antimicrobial Activity of Gramicidin A
Authors: Fang Shang-Ting , Huang Shu-Hsiang , Yang Chin-Hao , Liou Jen-Wen , Mani Hemalatha , Chen Yi-Cheng
Published: December 2022

Gramicidin A (gA) is a linear antimicrobial peptide that can form a channel and specifically conduct monovalent cations such as H+ across the lipid membrane. The antimicrobial activity of gA is associated with the formation of hydroxyl free radicals and the imbalance of NADH metabolism, possibly a consequence caused by the conductance of cations. The ion conductivity of gramicidin A can be blocked by Ca2+ ions. However, the effect of Ca2+ ions on the antimicrobial activity of gA is unclear. To unveil the role of Ca2+ ions, we examined the effect of Ca2+ ions on the antimicrobial activity of gramicidin A against Staphylococcus aureus (S. aureus). Results showed that the antimicrobial mechanism of gA and antimicrobial activity by Ca2+ ions are concentration-dependent. At the low gA concentration (≤1 μM), the antimicrobial mechanism of gA is mainly associated with the hydroxyl free radical formation and NADH metabolic imbalance. Under this mode, Ca2+ ions can significantly inhibit the hydroxyl free radical formation and NADH metabolic imbalance. On the other hand, at high gA concentration (≥5 μM), gramicidin A acts more likely as a detergent. Gramicidin A not only causes an increase in hydroxyl free radical levels and NAD+/NADH ratios but also induces the destruction of the lipid membrane composition. At this condition, Ca2+ ions can no longer reduce the gA antimicrobial activity but rather enhance the bacterial killing ability of gramicidin A.
PRR11 in Malignancies: Biological Activities and Targeted Therapies
Authors: Han Wei , Chen Liang
Published: December 2022

Proline rich 11 (PRR11), initially renowned for its relevance with cell-cycle progression, is a proline-rich protein coding gene in chromosome 17q22-23. Currently, accumulating studies have demonstrated that PRR11 plays a critical role in cellular proliferation, colony formation, migration, invasion, cell-cycle progression, apoptosis, autophagy and chemotherapy resistance via multiple signaling pathways and biological molecules in several solid tumors. In particular, PRR11 also serves as a promising prognostic indicator in a limited number of human cancers, gradually manifesting its potential application for targeted therapies. In this review, we summarize functional activities, related signaling pathways and biological molecules of PRR11 in various malignancies and generalize potential application of PRR11 for targeted therapies, thereby contributing to further exploration of PRR11 in cancer treatment.
IL-18BP Improves Early Graft Function and Survival in Lewis–Brown Norway Rat Orthotopic Liver Transplantation Model
Authors: Meng Qiang , Wu Weikang , Zhang Wenjie , Yuan Juzheng , Yang Long , Zhang Xuan , Tao Kaishan
Published: December 2022

Interleukin-18 (IL-18) can effectively activate natural killer (NK) cells and induce large concentrations of interferon-γ (IFN-γ). In healthy humans, IL-18 binding protein (IL-18BP) can inhibit the binding of IL-18 to IL-18R and counteract the biological action of IL-18 due to its high concentration and high affinity, thus preventing the production of IFN-γ and inhibiting NK-cell activation. Through previous studies and the phenomena observed by our group in pig–non-human primates (NHPs) liver transplantation experiments, we proposed that the imbalance in IL-18/IL-18BP expression upon transplantation encourages the activation, proliferation, and cytotoxic effects of NK cells, ultimately causing acute vascular rejection of the graft. In this research, we used Lewis–Brown Norway rat orthotopic liver transplantation (OLTx) as a model of acute vascular rejection. AAV8-Il18bp viral vectors as gene delivery vehicles were constructed for gene therapy to overexpress IL-18BP and alleviate NK-cell rejection of the graft after transplantation. The results showed that livers overexpressing IL-18BP had reduced damage and could function longer after transplantation, effectively improving the survival time of the recipients.
New Insights into lncRNAs in Aβ Cascade Hypothesis of Alzheimer’s Disease
Authors: Hao Yitong , Xie Bo , Fu Xiaoshu , Xu Rong , Yang Yu
Published: December 2022

Alzheimer’s disease (AD) is the most common type of dementia, but its pathogenesis is not fully understood, and effective drugs to treat or reverse the progression of the disease are lacking. Long noncoding RNAs (lncRNAs) are abnormally expressed and deregulated in AD and are closely related to the occurrence and development of AD. In addition, the high tissue specificity and spatiotemporal specificity make lncRNAs particularly attractive as diagnostic biomarkers and specific therapeutic targets. Therefore, an in-depth understanding of the regulatory mechanisms of lncRNAs in AD is essential for developing new treatment strategies. In this review, we discuss the unique regulatory functions of lncRNAs in AD, ranging from Aβ production to clearance, with a focus on their interaction with critical molecules. Additionally, we highlight the advantages and challenges of using lncRNAs as biomarkers for diagnosis or therapeutic targets in AD and present future perspectives in clinical practice.
SARS-CoV-2 Serology: Utility and Limits of Different Antigen-Based Tests through the Evaluation and the Comparison of Four Commercial Tests
Authors: Gdoura Mariem , Halouani Habib , Sahli Donia , Mrad Mehdi , Chamsa Wafa , Mabrouk Manel , Hogga Nahed , Ben-Salem Kamel , Triki Henda
Published: December 2022

Introduction: SARS-CoV-2 serology have several indications. Currently, as there are various types available, it is important to master their performance in order to choose the best test for the indication. We evaluated and compared four different commercial serology tests, three of them had the Food and Drug Administration Emergency Use Authorization (FDA-EUA). Our goal was to provide new data to help guide the interpretation and the choice of the serological tests. Methods: Four commercial tests were studied: Elecsys® Roche® on Cobas® (total anti-nucleocapsid (N) antibodies), VIDAS® Biomerieux® (IgM and IgG anti- receptor binding domain (RBD) antibodies), Mindray® (IgM and IgG anti-N and anti-RBD antibodies) and Access® Beckman Coulter® (IgG anti-RBD antibodies). Two panels were tested: a positive panel (n = 72 sera) obtained from COVID-19-confirmed patients with no vaccination history and a negative panel (n = 119) of pre-pandemic sera. The analytical performances were evaluated and the ROC curve was drawn to assess the manufacturer’s cut-off for each test. Results: A large range of variability between the tests was found. The Mindray®IgG and Cobas® tests showed the best overall sensitivity, which was equal to 79.2% CI 95% (67.9–87.8). The Cobas® test showed the best sensitivity after 14 days of COVID-19 molecular confirmation; which was equal to 85.4% CI 95% (72.2–93.9). The Access® test had a lower sensitivity, even after day 14 (55.5% CI 95% (43.4–67.3)). The best specificity was noted for the Cobas®, VIDAS®IgG and Access® IgG tests (100% CI 95% (96.9–100)). The IgM tests, VIDAS®IgM and Mindray®IgM, showed the lowest specificity and sensitivity rates. Overall, only 43 out of 72 sera (59.7%) showed concordant results by all tests. Retained cut-offs for a significantly better sensitivity and accuracy, without significant change in the specificity, were: 0.87 for Vidas®IgM (p = 0.01) and 0.14 for Access® (p < 10−4). The combination of Cobas® with Vidas® IgM and IgG offered the best accuracy in comparison with all other tests combinations. Conclusion: Although using an FDA-EUA approved serology test, each laboratory should carry out its own evaluation. Tests variability may raise some concerns that seroprevalence studies may vary significantly based on the used serology test.
Sex Hormones as Key Modulators of the Immune Response in Multiple Sclerosis: A Review
Authors: Murgia Federica , Giagnoni Florianna , Lorefice Lorena , Caria Paola , Dettori Tinuccia , D’Alterio Maurizio N. , Angioni Stefano , Hendren Aran J. , Caboni Pierluigi , Pibiri Monica , Monni Giovanni , Cocco Eleonora , Atzori Luigi
Published: December 2022

Background: A variety of autoimmune diseases, including MS, amplify sex-based physiological differences in immunological responsiveness. Female MS patients experience pathophysiological changes during reproductive phases (pregnancy and menopause). Sex hormones can act on immune cells, potentially enabling them to modify MS risk, activity, and progression, and to play a role in treatment. Methods: Scientific papers (published between 1998 and 2021) were selected through PubMed, Google Scholar, and Web of Science literature repositories. The search was limited to publications analyzing the hormonal profile of male and female MS patients during different life phases, in particular focusing on sex hormone treatment. Results: Both men and women with MS have lower testosterone levels compared to healthy controls. The levels of estrogens and progesterone increase during pregnancy and then rapidly decrease after delivery, possibly mediating an immune-stabilizing process. The literature examined herein evidences the neuroprotective effect of testosterone and estrogens in MS, supporting further examinations of their potential therapeutic uses. Conclusions: A correlation has been identified between sex hormones and MS clinical activity. The combination of disease-modifying therapies with estrogen or estrogen plus a progestin receptor modulator promoting myelin repair might represent an important strategy for MS treatment in the future.
Generation of Endogenous Promoter-Driven Luciferase Reporter System Using CRISPR/Cas9 for Investigating Transcriptional Regulation of the Core Clock Gene BMAL1
Authors: Sun Chengxi , Li Chen , Liu Wen , Schiöth Helgi B.
Published: December 2022

Humans and other organisms are continuously exposed to thousands of chemicals through the atmosphere, drinking water, food, or direct contact. A large proportion of such chemicals are present in very low concentrations and may have synergistic effects, even at their no-observed-adverse-effect level (NOAEL). Complex mixtures of contaminants are very difficult to assess by traditional toxicological methods. There is increasing attention on how different pollutants induce adverse physiological functions in the human body through effects on the circadian rhythm. However, it is very difficult to screen for compounds with circadian-rhythm-disrupting effects from a large number of chemicals or their complex mixtures. We established a stable firefly luciferase reporter gene knock-in U2-OS cell line by CRISPR/Cas9 to screen circadian-rhythm-disrupting pollutants. The luciferase gene was inserted downstream of the core clock gene BMAL1 and controlled by an endogenous promoter. Compared to detection systems using exogenous promoters, these cells enable the detection of compounds that interfere with the circadian rhythm system mediated by BMAL1 gene expression. The U2-OS knock-in cells showed BMAL1 and luciferase activity had parallel changes when treated with BMAL1 inhibitor and activator. Furthermore, the luciferase reporter gene has high sensitivity and is faster and more cost-effective than classic toxicology methods. The knock-in cell line can be used for high-throughput and efficient screening of circadian-rhythm-disrupting chemicals such as drugs and pollutants.
Serum Levels of Eosinophil-Derived Neurotoxin, Platelet-Activating Factor and Vascular Endothelial Growth Factor in Adult Patients with Atopic Dermatitis—A Pilot Study
Authors: Gomułka Krzysztof , Wójcik Ewa , Szepietowski Jacek Cezary
Published: December 2022

Atopic dermatitis (AD) is a chronic, highly pruritic, relapsing–remitting inflammatory skin disease. The etiology of AD has not been fully explained yet and complex interactions of various small molecules are still being taken into account. The aim of this research was to investigate the serum eosinophil-derived neurotoxin (EDN), platelet activating factor (PAF) and vascular endothelial growth factor (VEGF) concentrations in relation to the disease severity and pruritus intensity in adult patients with AD. This pilot study was performed on 30 participants (15 patients with AD and 15 healthy controls). Blood samples were taken to examine the serum levels of EDN, PAF and VEGF using the enzyme-linked immunosorbent assay (ELISA) test. The severity of disease was assessed by the Scoring Atopic Dermatitis (SCORAD) index. The intensity of pruritus, as a subjective symptom, was determined by the Visual Analogue Scale (VAS). Obtained results revealed that the EDN (p = 0.016) and VEGF (p = 0.032), but not PAF (p = 0.841) concentrations were significantly higher in patients with AD compared with those of the control group. There was positive correlation between the EDN level and the SCORAD index in patients with AD (r = −0.9, p = 0.037) which was not found for the PAF and VEGF levels. Circulating EDN, PAF and VEGF levels were not significantly correlated with the severity of pruritus. Our results suggest that the END and VEGF serum levels are significantly increased in patients with AD compared to control group. Moreover, EDN might be useful to reflect the severity of symptoms.
Understanding Long COVID; Mitochondrial Health and Adaptation—Old Pathways, New Problems
Authors: Nunn Alistair V. W. , Guy Geoffrey W. , Brysch Wolfgang , Bell Jimmy D.
Published: December 2022

Many people infected with the SARS-CoV-2 suffer long-term symptoms, such as “brain fog”, fatigue and clotting problems. Explanations for “long COVID” include immune imbalance, incomplete viral clearance and potentially, mitochondrial dysfunction. As conditions with sub-optimal mitochondrial function are associated with initial severity of the disease, their prior health could be key in resistance to long COVID and recovery. The SARs virus redirects host metabolism towards replication; in response, the host can metabolically react to control the virus. Resolution is normally achieved after viral clearance as the initial stress activates a hormetic negative feedback mechanism. It is therefore possible that, in some individuals with prior sub-optimal mitochondrial function, the virus can “tip” the host into a chronic inflammatory cycle. This might explain the main symptoms, including platelet dysfunction. Long COVID could thus be described as a virally induced chronic and self-perpetuating metabolically imbalanced non-resolving state characterised by mitochondrial dysfunction, where reactive oxygen species continually drive inflammation and a shift towards glycolysis. This would suggest that a sufferer’s metabolism needs to be “tipped” back using a stimulus, such as physical activity, calorie restriction, or chemical compounds that mimic these by enhancing mitochondrial function, perhaps in combination with inhibitors that quell the inflammatory response.
Picomolar or beyond Limit of Detection Using Molecularly Imprinted Polymer-Based Electrochemical Sensors: A Review
Authors: Shah Naheed Sidiq , Thotathil Vandana , Zaidi Shabi Abbas , Sheikh Hanan , Mohamed Maimoona , Qureshi Ahmadyar , Sadasivuni Kishor Kumar
Published: December 2022

Over the last decades, molecularly imprinted polymers (MIPs) have emerged as selective synthetic receptors that have a selective binding site for specific analytes/target molecules. MIPs are synthetic analogues to the natural biological antigen–antibody system. Owing to the advantages they exhibit, such as high stability, simple synthetic procedure, and cost-effectiveness, MIPs have been widely used as receptors/sensors for the detection and monitoring of a variety of analytes. Moreover, integrating electrochemical sensors with MIPs offers a promising approach and demonstrates greater potential over traditional MIPs. In this review, we have compiled the methods and techniques for the production of MIP-based electrochemical sensors along with the applications of reported MIP sensors for a variety of analytes. A comprehensive in-depth analysis of recent trends reported on picomolar (pM/10−12 M)) and beyond picomolar concentration LOD (≥pM) achieved using MIPs sensors is reported. Finally, we discuss the challenges faced and put forward future perspectives along with our conclusion.
Tracking a Major Egg Allergen to Assess Commercial Food Label Compliance: Towards a Simple and Fast Immunosensing Device
Authors: Freitas Maria , del Rio Mariana , Nouws Henri P. A. , Delerue-Matos Cristina
Published: December 2022

An amperometric immunosensor was developed for the analysis of the major egg-white allergen ovotransferrin (Gal d 3) in commercial food products because the (accidental) intake, skin contact with, and/or inhalation of eggs can lead to severe disorders in allergic individuals. Employing a sandwich-type immunosensing strategy, screen-printed carbon electrodes (SPCE) were biomodified with anti-Gal d 3 (capture) antibodies, and the allergen’s detection was achieved with anti-Gal d 3 antibodies labelled with horseradish peroxidase (HRP). The 3,3′,5,5′-tetramethylbenzidine (TMB)/H2O2 reaction with HRP was used to obtain the electrochemical (amperometric) signal. An attractive assay time of 30 min and a remarkable analytical performance was achieved. The quantification range was established between 55 and 1000 ng·mL−1, with a limit of detection of 16 ng·mL−1. The developed method demonstrated good precision (Vx0 = 5.5%) and provided precise results (CV < 6%). The sensor also detected extremely low amounts (down to 0.010%) of egg. The analysis of seven raw and/or cooked egg and egg-white samples indicated that food processing influences the amount of allergen. Furthermore, to assure the compliance of product labelling with EU legislation, 25 commercial food ingredients/products were analysed. The accuracy of the results was confirmed through an ELISA assay. The stability of the ready-to-use sensing surface for 20 days allows a reduction of the reagents’ volumes and cost.
Development and Validation of the Digital Life Balance (DLB) Scale: A Brand-New Measure for Both Harmonic and Disharmonic Use of ICTs
Authors: Duradoni Mirko , Serritella Elena , Avolio Claudia , Arnetoli Claudio , Guazzini Andrea
Published: December 2022

The use of new technologies and information communication technology services (ICTs) has greatly increased, especially after the COVID-19 pandemic, resulting in an irrevocable change in people’s work-life balance (WLB). Despite the thriving literature on the dysfunctional use of new technologies, a functional use of ICTs also seems to be possible. Inspired by the theory of psychology of harmony and referring to behavioral addiction models and substance use models, we defined the construct of digital life balance to indicate a harmonic balance between digital life and real life. In this context, the imbalance between online and offline life may reflect a dysfunctional use of ICTs and can be seen as a process of disharmonization. With this perspective in mind, the aim of this study was to develop a dedicated measuring instrument that could capture both people’s balanced and unbalanced use of ICTs. Through two cross-sectional studies (Study 1= 1473 participants; Study 2 = 953 participants), we validated the scale internally and externally. In line with the literature, Digital Life Balance scores appeared to be negatively associated with addiction measures and positively associated with well-being measures. In conclusion, the Digital Life Balance (DLB) Scale appears to be a reliable (ω = 0.89) and valid instrument to investigate people’s harmonic and disharmonic use of ICTs.
A Comparison of Different Machine Learning Algorithms in the Classification of Impervious Surfaces: Case Study of the Housing Estate Fort Bema in Warsaw (Poland)
Authors: Sobieraj Janusz , Fernández Marcos , Metelski Dominik
Published: December 2022

The aim of this study is to extract impervious surfaces and show their spatial distribution, using different machine learning algorithms. For this purpose, geoprocessing and remote sensing techniques were used and three classification methods for digital images were compared, namely Support Vector Machines (SVM), Maximum Likelihood (ML) and Random Trees (RT) classifiers. The study area is one of the most prestigious and the largest housing estates in Warsaw (Poland), the Fort Bema housing complex, which is also an exemplary model for hydrological solutions. The study was prepared on the Geographic Information System platform (GIS) using aerial optical images, orthorectified and thus provided with a suitable coordinate system. The use of these data is therefore supported by the accuracy of the resulting infrared channel product with a pixel size of 0.25 m, making the results much more accurate compared to satellite imagery. The results of the SVM, ML and RT classifiers were compared using the confusion matrix, accuracy (Root Mean Square Error /RMSE/) and kappa index. This showed that the three algorithms were able to successfully discriminate between targets. Overall, the three classifiers had errors, but specifically for impervious surfaces, the highest accuracy was achieved with the SVM classifier (the highest percentage of overall accuracy), followed by ML and RT with 91.51%, 91.35% and 84.52% of the results, respectively. A comparison of the visual results and the confusion matrix shows that although visually the RT method appears to be the most detailed classification into pervious and impervious surfaces, the results were not always correct, e.g., water/shadow was detected as an impervious surface. The NDVI index was also mapped for the same spatial study area and its application in the evaluation of pervious surfaces was explained. The results obtained with the GIS platform, presented in this paper, provide a better understanding of how these advanced classifiers work, which in turn can provide insightful guidance for their selection and combination in real-world applications. The paper also provides an overview of the main works/studies dealing with impervious surface mapping, with different methods for their assessment (including the use of conventional remote sensing, NDVI, multisensory and cross-source data, ‘social sensing’ and classification methods such as SVM, ML and RT), as well as an overview of the research results.
An Integrated User Interface of Assessment and Optimization for Architectural Façade Shading Designs in Taiwan
Authors: Tsay Yaw-Shyan , Wu Min-Shiun , Lin Chuan-Hsuan
Published: December 2022

In response to sustainable development goals, the architectural industry aims to decrease the high proportion of emissions and energy use in the construction sector. Therefore, the design method of building performance optimization (BPO) has been advocated in recent studies as a method for accomplishing high-performance building design. However, BPO remains difficult to implement in practice due to the lack of a definite process and supporting tools for architects/designers in the early design process. The purpose of this paper is to propose a BPO framework and integrated design decision support (DDS) interface to provide a visual and science-based analysis and assist designers working with high-performance building façade designs. The framework and DDS tool are then tested by designers through a practice design of the headquarters façade. All the designers started and implemented the facade optimization design in a short training session, although they reported that the developed support tools still needed to be improved in terms of also integrating optimization tools. The characteristics of the user interface help considerably with comparing and making decisions in optimal solutions. The results emphasize the importance of developing design support tools for practical adoption from practical designers’ perspectives.
Polyethylene (PE) Waste Minimization Study of Cement Mortar with Adding PE Content under Different W/B Ratios
Authors: Tsai Shen-Lun , Lin Keng-Ta , Hung Chang-Chi , Wang Her-Yung , Wen Fu-Lin
Published: December 2022

Wastes can be effectively used in concrete and the characteristics of concrete can be maintained or enhanced, the economy of waste management can be greatly increased, and the pollution of the earth can be reduced. This study aimed to research the durability of cement mortar prepared using different W/B ratios and different percentages of waste PE content. The cement mortar was mixed with 0%, 1%, 2%, 3%, and 4% waste PE and 20% ground-granulated blast-furnace slag (GGBFS) in W/B ratios of 0.4, 0.5, and 0.6. The results sw that the slump and flow decrease as the waste PE content is increased and increase with increasing W/B ratio, and the setting time is shortened as the waste PE content is increased. In terms of hardened properties, the specimen strength is slightly decreased as the waste PE content is increased, but the hardened properties are better at a later age due to the pozzolanic reaction of slag, which can be verified by microscopic SEM.
Lymphocyte Classification from Hoechst Stained Slides with Deep Learning
Authors: Cooper Jessica , Um In Hwa , Arandjelović Ognjen , Harrison David J.
Published: December 2022

Multiplex immunofluorescence and immunohistochemistry benefit patients by allowing cancer pathologists to identify proteins expressed on the surface of cells. This enables cell classification, better understanding of the tumour microenvironment, and more accurate diagnoses, prognoses, and tailored immunotherapy based on the immune status of individual patients. However, these techniques are expensive. They are time consuming processes which require complex staining and imaging techniques by expert technicians. Hoechst staining is far cheaper and easier to perform, but is not typically used as it binds to DNA rather than to the proteins targeted by immunofluorescence techniques. In this work we show that through the use of deep learning it is possible to identify an immune cell subtype without immunofluorescence. We train a deep convolutional neural network to identify cells expressing the T lymphocyte marker CD3 from Hoechst 33342 stained tissue only. CD3 expressing cells are often used in key prognostic metrics such as assessment of immune cell infiltration, and by identifying them without the need for costly immunofluorescence, we present a promising new approach to cheaper prediction and improvement of patient outcomes. We also show that by using deep learning interpretability techniques, we can gain insight into the previously unknown morphological features which make this possible.
Clinical, Pathological, and Molecular Characteristics in Colorectal Cancer
Authors: Dedieu Stéphane , Bouché Olivier
Published: December 2022

Colorectal cancer (CRC) is the third most diagnosed cancer worldwide, and the second leading cause of death in patients with cancer [...]
Crystal Contact Engineering for Enhanced Cross-Linking Efficiency of HheG Crystals
Authors: Staar Marcel , Staar Sophie , Schallmey Anett
Published: December 2022

The generation of cross-linked enzyme crystals is a very attractive method for immobilization of enzymes displaying high crystalizability. However, the commonly used cross-linker glutaraldehyde is not always compatible with enzyme activity. Therefore, we previously reported the engineering of halohydrin dehalogenase HheG from Ilumatobacter coccineus to enable thiol-specific cross-linking during CLEC generation by insertion of cysteine residues in the crystal contact. To broaden the applicability of this approach, herein crystal contact engineering of HheG has been performed to incorporate additional lysine residues as defined cross-linking sites for CLEC generation. Using the primary amine-specific cross-linker dithiobis(succinimidyl propionate) (DSP), CLECs of HheG variant V46K were obtained that displayed a high gain in thermal stability compared to wild-type HheG, while using only a low cross-linker concentration. Moreover, respective V46K CLECs exhibited a 10 K higher reaction temperature optimum as well as significantly improved activity and stability at acidic pH and in the presence of organic co-solvents. Overall, our study demonstrates that lysine-specific cross-linkers can also be used as an alternative to glutaraldehyde for stable CLEC generation of halohydrin dehalogenases, and that cross-linking efficiency is significantly improved upon crystal contact engineering.
Sensor Based on a Solid Oxide Electrolyte for Measuring the Water-Vapor and Hydrogen Content in Air
Authors: Kalyakin Anatoly , Demin Anatoly K. , Gorbova Elena , Volkov Alexander , Tsiakaras Panagiotis E.
Published: December 2022

The present communication describes the results of the performance and the assessment of a sensor based on a solid oxide electrolyte with a composition of 0.9ZrO2 + 0.1Y2O3 (YSZ), equipped with a ceramic diffusion barrier for measuring the water vapor and hydrogen content in air. The possibility of determining the concentration of water vapor and hydrogen in the air is based on the measurement of the limiting current value. For the calculation of the steam and hydrogen concentration in ambient air, analytical expressions were obtained and applied, using the limiting current values measured in air with a standard oxygen concentration of 20.9 vol.% and in the analyzed air. A two-stage method for the determination of the hydrogen and steam amount in ambient air is proposed. It is stated that the sensor operates successfully at the temperature of 700 °C and can be applied for the continuous determination of steam or hydrogen concentrations in air.
Normoxic HIF-1α Stabilization Caused by Local Inflammatory Factors and Its Consequences in Human Coronary Artery Endothelial Cells
Authors: Abdi Sarabi Mohsen , Shiri Alireza , Aghapour Mahyar , Reichardt Charlotte , Brandt Sabine , Mertens Peter R. , Medunjanin Senad , Bruder Dunja , Braun-Dullaeus Ruediger C. , Weinert Sönke
Published: December 2022

Knowledge about normoxic hypoxia-inducible factor (HIF)-1α stabilization is limited. We investigated normoxic HIF-1α stabilization and its consequences using live cell imaging, immunoblotting, Bio-Plex multiplex immunoassay, immunofluorescence staining, and barrier integrity assays. We demonstrate for the first time that IL-8 and M-CSF caused HIF-1α stabilization and translocation into the nucleus under normoxic conditions in both human coronary endothelial cells (HCAECs) and HIF-1α-mKate2-expressing HEK-293 cells. In line with the current literature, our data show significant normoxic HIF-1α stabilization caused by TNF-α, INF-γ, IL-1β, and IGF-I in both cell lines, as well. Treatment with a cocktail consisting of TNF-α, INF-γ, and IL-1β caused significantly stronger HIF-1α stabilization in comparison to single treatments. Interestingly, this cumulative effect was not observed during simultaneous treatment with IL-8, M-CSF, and IGF-I. Furthermore, we identified two different kinetics of HIF-1α stabilization under normoxic conditions. Our data demonstrate elevated protein levels of HIF-1α-related genes known to be involved in the development of atherosclerosis. Moreover, we demonstrate an endothelial barrier dysfunction in HCAECs upon our treatments and during normoxic HIF-1α stabilization comparable to that under hypoxia. This study expands the knowledge of normoxic HIF-1α stabilization and activation and its consequences on the endothelial secretome and barrier function. Our data imply an active role of HIF-1α in vivo in the vasculature in the absence of hypoxia.