bluuebunny/crossref_metadata_2025 huggingface dataset:
Growth Performance and Immunity of Broilers Fed Sorghum–Soybean Meal Diets Supplemented with Phytases and Β-Mannanases
Authors: Sastré-Calderón Nicolás , Gómez-Verduzco Gabriela , Cortés-Cuevas Arturo , Juárez-Ramírez Mireya , Arce-Menocal José , Márquez-Mota Claudia Cecilia , Sánchez-Godoy Félix , Ávila-González Ernesto
Published: March 2024

Most grains and vegetable feedstuffs used in commercial poultry feed contain phytates and polysaccharides—non-starchy chemical structures that are not degraded by digestive tract enzymes. Exogenous enzymes optimize the use of dietary ingredients. This study aimed to determine whether combining β-mannanases (400 g/ton) and phytases in broiler sorghum–soybean diets could improve performance and immunity in broilers. Four diets were randomized in a 2 × 2 factorial design, with two phytase levels (500 or 1500 FTU/kg) and β-mannanase supplementation (0–400 g/ton; 158 million units/kg minimum enzyme activity). Six replicate battery cages of 10 chicks were fed each diet ad libitum. To assess cellular and humoral immune responses, 10 birds per treatment were euthanized on day 21. Supplementation with β-mannanase enzymes led to increased body weight and a higher feed conversion index (FCI) (p < 0.05). The phytase factor improved the FCI at 1500 FTU/kg (p < 0.05). Supplementation with β-mannanases improved the immune response by increasing the IgA concentration in the duodenum (95%) and total serum immunoglobulins (p < 0.05). The morphometric index increased in all organs (p < 0.05), and the heterophile/lymphocyte ratio (HLR) decreased by 50% (p < 0.05). Supplementing broilers with β-mannanases in sorghum–soybean meal diets with phytases improved their performance and immunity.
Effects of Elective Caesarean Sections in Healthy Near-Term Ewes on Subsequent Reproductive Performance
Authors: Voigt Katja , Theisges Mara , Zablotski Yury , Weber Frank , Zerbe Holm
Published: March 2024

Post-surgical reproductive performance following ovine caesarean section has not been well studied. To assess any direct effects of surgical delivery in the absence of confounders such as dystocia or underlying diseases, we studied elective surgery performed in healthy animals for teaching purposes. Four hundred and eleven paired breeding records following vaginal delivery (n = 233), elective caesarean section (n = 122), and subsequent further vaginal deliveries in animals with a history of one prior elective caesarean operation (n = 56) were evaluated retrospectively. The overall subsequent pregnancy rate was 95%. Multivariable statistical analyses did not reveal any significant influence of planned caesarean surgery on subsequent conception, stillbirth, perinatal lamb mortality, lamb birth weights, or the incidence of premature foetal death (mummification and abortion). A significantly higher number of mating attempts was, however, necessary. Also, a significant reduction in litter size was seen in the first pregnancy immediately following a surgical delivery in comparison to animals which had previously only delivered vaginally (p = 0.001), but litter size returned to pre-caesarean levels in further follow-up pregnancies in animals with a history of one elective caesarean section (p = 0.436). Subsequent long-term reproductive performance of sheep following elective caesarean section is thus excellent, and the results encourage retention for breeding.
Dietary Supplementation with Bupleuri Radix Reduces Oxidative Stress Occurring during Growth by Regulating Rumen Microbes and Metabolites
Authors: Pan Cheng , Li Haiyan , Wang Fuqiang , Qin Jianping , Huang Yanping , Zhao Wangsheng
Published: March 2024

Oxidative stress (OS) in ruminants is closely associated with disease; thus, improving antioxidant capacity is an important strategy for maintaining host health. Bupleuri Radix (BR) could significantly improve host health and stress levels. However, the clear antioxidant mechanism of the function of BR remains unknown. In the current study, LC-MS metabolomics combined with 16S rRNA gene sequencing was employed to explore the effects of BR on rumen microbiota and metabolites in Shanbei Fine-Wool Sheep (SFWS), and Spearman correlation analyses of rumen microbiota, metabolites, and OS were performed to investigate the mechanism of antioxidant function of BR. Our results indicated that as SFWS grows, levels of OS and antioxidant capacity increase dramatically, but providing BR to SFWS enhances antioxidant capacity while decreasing OS. Rumen microbiota and OS are strongly correlated, with total antioxidant capacity (T-AOC) showing a significant negative correlation with Succiniclasticum and a positive correlation with Ruminococcus. Importantly, the Chao1 index was significantly negatively correlated with malondialdehyde (MDA) and positively correlated with superoxide dismutase (SOD) and T-AOC. Two biomarkers connected to the antioxidant effects of BR, 5,6-DHET and LPA (a-25:0/0:0), were screened according to the results of metabolomics and Spearman analysis of rumen contents, and a significant relationship between the concentration of rumen metabolites and OS was found. Five metabolic pathways, including glycerolipid, glutathione, nucleotide, D-amino acid, and inositol phosphate metabolism, may have a role in OS. The integrated results indicate that rumen microbiota and metabolites are strongly related to OS and that BR is responsible for reducing OS and improving antioxidant capacity in post-weaned SFWS. These findings provide new strategies to reduce OS occurring during SFWS growth.
Whole-Genome Identification and Characterization of the DKK Gene Family and Its Transcription Profiles: An Analysis of the Chinese Soft-Shell Turtle (Pelodiscus sinensis)
Authors: Wang Yongchang , Zhu Junxian , Chen Chen , Ji Liqin , Hong Xiaoyou , Liu Xiaoli , Chen Haigang , Wei Chengqing , Zhang Junjie , Zhu Xinping , Li Wei
Published: March 2024

The DKK family is a canonical small family of WNT antagonists. Though recent studies have suggested that the DKK gene family may be involved in sex differentiation in Pelodiscus sinensis, there are still a lot of things about the DKK gene family that we do not know. In this study, we used bioinformatics methods to identify members of the DKK gene family in P. sinensis and analyzed their phylogeny, covariance, gene structure, structural domains, promoter conserved sites, signal peptides, gonadal transcription factors, transcriptional profiles, and tissue expression profiles. Additionally, qRT-PCR results were utilized for the validation and preliminary investigation of the function of the DKK gene family in P. sinensis. The results showed that the DKK gene family is divided into six subfamilies, distributed on six different chromosomal scaffolds containing different gene structures and conserved motifs with the same structural domains, and all of the members were secreted proteins. Our transcriptional profiling and embryonic expression analysis showed that DKKL1 and DKK4 were significantly expressed in the testes, whereas DKK1 and DKK3 were significantly upregulated in the ovaries. This suggests a potential function in sex differentiation in P. sinensis. Our results may provide a basic theoretical basis for the sex differentiation process in P. sinensis.
Comparative Analysis of Ascaris suum and Macracanthorhynchus hirudinaceus Infections in Free-Ranging and Captive Wild Boars (Sus scrofa) in Hungary
Authors: Farkas Csaba , Juhász Alexandra , Fekete Balázs , Egri Borisz
Published: March 2024

Ascaris suum and Macracanthorhynchus hirudinaceus cause a large loss of yield in farm animals as well as in free-living and captive wild boar herds, thereby causing economic damage. This study compared A. suum and M. hirudinaceus infections in free-ranging and captive wild boars (Sus scrofa) in Hungary. The authors measured the A. suum and M. hirudinaceus infections of a 248-hectare wild boar garden and an 11,893-hectare free-living wild boar herd in the sample area. In all cases, samples were collected from shot wild boars. In total, 216 wild boars were examined from June 2015 to June 2023 in Hungary. Of the 173 dissected wild boars from the wild, 57 (32.9%) were infected with A. suum, while 30 (69.8%) of the 43 individuals from the captive area were infected. The prevalence of M. hirudinaceus in the free-living area population was 9.25% (16 wild boars), while that of the captive population was 34.89% (15 wild boars). In the case of the examined helminths, the captive herd was 36.9% more infected than the herd living in the open area.
Nutrient and Energy Apparent Digestibility of Protein-Based Feed Ingredients and Effect of the Dietary Factors on Growth Performance and Feed Utilization of Sobaity Seabream, Sparidentex hasta
Authors: Zehra Seemab , Laranja Joseph Leopoldo Q. , Abulkasim Aboobucker Siddik , Saleh Reda , De Mello Paulo H. , Pantanella Edoardo , Alarcon Jorge , Al-Suwailem Abdulaziz M. , Al Shaikhi A. , Glencross Brett D. , Mohamed Asaad H. W.
Published: March 2024

Two separate feeding trials were undertaken to benchmark a series of commercial diets and determine the nutrient and energy apparent digestibility coefficients of a variety of protein-based feed ingredients when fed to sobaity seabream, Sparidentex hasta. In Experiment 1, triplicate groups of fish (initial body weight: 330.5 ± 2.6 g) were fed with one of three locally available diets containing crude protein (CP) levels ranging from 44 to 46% of dry matter (DM), each with ~12% crude fat. Fish grew at around 3.2 g day−1 with a specific growth rate (SGR) of 0.7% day−1. Both the feed conversion ratio (FCR) and protein efficiency ratio (PER) were significantly better in fish fed diets, which contained the highest (46.4%) crude protein level. Overall, the data from these preliminary studies suggest that the best performance by sobaity seabream was obtained with a diet containing 46% crude protein, 20 MJ/kg, and a protein-to-energy ratio of 23 mg/kJ. In Experiment 2, fish with an initial body weight of 319 ± 7 g were held in 11 tanks and fed reference (D1) and test diets (D2–D11) for 7 days before fecal collection. This process was repeated twice in a blocking arrangement to generate three replicates. Each of the ten test diets contained 30% of a test ingredient, with the remaining 70% proportionally identical to the D1 diet. Diet apparent digestibility coefficients (ADCs) were measured, and the diet ADCs were then used to derive the protein and energy ADCs for the individual test ingredients. Ingredient protein ADC ranged between 75.5 and 93.9%, while ingredient energy ADC ranged between 66.8 and 81.2%.
Effect of Diluents and Storage Time on the Cryopreservation of Collared Peccary (Pecari tajacu) Semen after Cooling Storage in a Transport Container at 5 °C
Authors: Santos Romário P. , Silva Andréia M. , Pereira Ana G. , Cavalcante Yasmim C. S. , Matos Yuri G. , Bezerra Gabriel S. C. , Dantas Lilian L. , Silva Alexandre R.
Published: March 2024

We verified the possibility of cooling peccary semen for 4, 24, and 48 h before cryopreservation, using different dilution media (TRIS + egg yolk (20%) and PRIMXcell Ultra). Ten ejaculates were divided equally into six aliquots and then diluted. Two aliquots were stored in a biological incubator (4 h), and the remaining aliquots were stored in a commercial container, the Botutainer® (24 and 48 h), both at 5 °C. The samples were cryopreserved and then evaluated for kinetic parameters, functionality, integrity, mitochondrial activity, morphology, and sperm binding capacity. After thawing, samples diluted in TRIS showed total motility of 43.4 ± 6.8%, 48.4 ± 6.2%, and 38.6 ± 5.0% after cooling for 4, 24, and 48 h before cryopreservation, respectively. Such results are significantly greater than those achieved with the use of PRIMXcell diluent for 4 (8.3 ± 2.8%), 24 (4.7 ± 1.4%), and 48 h (4.8 ± 2.9%) storage (p < 0.05). Furthermore, TRIS provided better preservation of sperm membrane integrity when samples were cooled for 24 h (44.5 ± 4.7%) before cryopreservation compared to those samples diluted in PRIMXcell Ultra stored for 24 (25.7 ± 4.0%) and 48 h (25.2 ± 4.0%) before freezing (p < 0.05). In summary, we suggest TRIS diluent + egg yolk (20%) as an effective option to allow semen to cool for 24 or 48 h in a transport container before cryopreservation.
Generation and Characterization of Stable Small Colony Variants of USA300 Staphylococcus aureus in RAW 264.7 Murine Macrophages
Authors: Bivona Dalida , Bonomo Carmelo , Colombini Lorenzo , Bonacci Paolo G. , Privitera Grete F. , Caruso Giuseppe , Caraci Filippo , Santoro Francesco , Musso Nicolò , Bongiorno Dafne , Iannelli Francesco , Stefani Stefania
Published: March 2024

Intracellular survival and immune evasion are typical features of staphylococcal infections. USA300 is a major clone of methicillin-resistant S. aureus (MRSA), a community- and hospital-acquired pathogen capable of disseminating throughout the body and evading the immune system. Carnosine is an endogenous dipeptide characterized by antioxidant and anti-inflammatory properties acting on the peripheral (macrophages) and tissue-resident (microglia) immune system. In this work, RAW 264.7 murine macrophages were infected with the USA300 ATCC BAA-1556 S. aureus strain and treated with 20 mM carnosine and/or 32 mg/L erythromycin. Stable small colony variant (SCV) formation on blood agar medium was obtained after 48 h of combined treatment. Whole genome sequencing of the BAA-1556 strain and its stable derivative SCVs when combining Illumina and nanopore technologies revealed three single nucleotide differences, including a nonsense mutation in the shikimate kinase gene aroK. Gene expression analysis showed a significant up-regulation of the uhpt and sdrE genes in the stable SCVs compared with the wild-type, likely involved in adaptation to the intracellular milieu.
Identification and Clinical Characteristics of Community-Acquired Acinetobacter baumannii in Patients Hospitalized for Moderate or Severe COVID-19 in Peru
Authors: Silva-Caso Wilmer , Pérez-Lazo Giancarlo , Aguilar-Luis Miguel Angel , Morales-Moreno Adriana , Ballena-López José , Soto-Febres Fernando , Martins-Luna Johanna , Del Valle Luis J. , Kym Sungmin , Aguilar-Luis Deysi , Denegri-Hinostroza Dayana , del Valle-Mendoza Juana
Published: March 2024

Acinetobacter baumannii has been described as a cause of serious community-acquired infections in tropical countries. Currently, its implications when simultaneously identified with other pathogens are not yet adequately understood. A descriptive study was conducted on hospitalized patients with a diagnosis of moderate/severe SARS-CoV-2-induced pneumonia confirmed via real-time RT-PCR. Patients aged > 18 years who were admitted to a specialized COVID-19 treatment center in Peru were selected for enrollment. A. baumannii was detected via the PCR amplification of the blaOXA-51 gene obtained from nasopharyngeal swabs within 48 h of hospitalization. A total of 295 patients with COVID-19 who met the study inclusion criteria were enrolled. A. baumannii was simultaneously identified in 40/295 (13.5%) of COVID-19-hospitalized patients. Demographic data and comorbidities were comparable in both Acinetobacter-positive and -negative subgroups. However, patients identified as being infected with Acinetobacter were more likely to have received outpatient antibiotics prior to hospitalization, had a higher requirement for high-flow nasal cannula and a higher subjective incidence of fatigue, and were more likely to develop Acinetobacter-induced pneumonia during hospitalization. Conclusions: The group in which SARS-CoV-2 and A. baumannii were simultaneously identified had a higher proportion of fatigue, a higher frequency of requiring a high-flow cannula, and a higher proportion of superinfection with the same microorganism during hospitalization.
A Comprehensive Study on the Antibacterial Activities of Carbon Quantum Dots Derived from Orange Juice against Escherichia coli
Authors: Nguyen Minh Hoa , Le Anh Thi , Pham Van Duong , Pham Hong Minh , Do Hoang Tung , Le Duc Toan , Vu Thi Bich , Nguyen Thanh Binh
Published: March 2024

Carbon quantum dots (CQDs) are known for their intriguing optical properties, low toxicity, and high biocompatibility, which make them promising for biomedical applications. In this study, CQDs were synthesized by subjecting orange juice to microplasma as a carbon source at atmospheric pressure and low temperatures. The resulting CQDs exhibited a narrow size distribution, with an average diameter of approximately 4.5 nm and a pH value of 5.67. These CQDs exhibited strong blue emission characteristics. The antibacterial properties of the CQDs against Escherichia coli (E. coli) strains were evaluated using minimum inhibitory concentration assays. The study revealed that an effective inhibition of E. coli was achieved at a minimum inhibitory concentration of 0.1 ppm, while the minimum bactericidal concentration for this bacterial strain was 1 ppm, resulting in an average antibacterial efficacy of 57%. Notably, the antibacterial effects of the CQDs were observed without the need for additional light or oxidants, demonstrating the applicability of CQDs in combating bacterial strains.
Performance Analysis of the Particle Swarm Optimization Algorithm in a VLC System for Localization in Hospital Environments
Authors: Candia Diego Alonso , Játiva Pablo Palacios , Azurdia Meza Cesar , Sánchez Iván , Ijaz Muhammad
Published: March 2024

Localization in hospitals can be valuable in improving different services in medical environments. In this sense, an accurate location system in this environment requires adequately enabling communication technology. However, widely adopted technologies such as Wireless Fidelity (WiFi), Bluetooth, and Radio Frequency Identification (RFID) are considered poorly suited to enable hospital localization due to their inherent drawbacks, including high implementation costs, poor signal strength, imprecise estimates, and potential interference with medical devices. The increasing expenses associated with the implementation and maintenance of these technologies, along with their limited accuracy in dynamic hospital environments, underscore the pressing need for alternative solutions. In this context, it becomes imperative to explore and present novel approaches that not only avoid these challenges but also offer more cost effective, accurate, and interference-resistant connectivity to achieve precise localization within the complex and sensitive hospital environment. In the quest to achieve adequate localization accuracy, this article strategically focuses on leveraging Visible Light Communication (VLC) as a fundamental technology to address the specific demands of hospital environments to achieve the precise localization and tracking of life-saving equipment. The proposed system leverages existing lighting infrastructure and utilizes three transmitting LEDs with different wavelengths. The Received Signal Strength (RSS) is used at the receiver, and a trilateration algorithm is employed to determine the distances between the receiver and each LED to achieve precise localization. The accuracy of the localization is further enhanced by integrating a trilateration algorithm with the sophisticated Particle Swarm Optimization (PSO) algorithm. The proposed method improves the localization accuracy, for example, at a height of 1 m, from a 11.7 cm error without PSO to 0.5 cm with the PSO algorithm. This enhanced accuracy is very important to meet the need for precise equipment location in dynamic and challenging hospital environments to meet the demand for life-saving equipment. Furthermore, the performance of the proposed localization algorithm is compared with conventional positioning methods, which denotes improvements in terms of the localization error and position estimation.
Evolution Characteristics of the Strength and Dilation Parameters of Gas Hydrate-Bearing Sediments and a Modeling Study
Authors: Yu Jincai , Cheng Cheng , Yang Jintao
Published: March 2024

Gas hydrate has gradually become a new potential energy resource. However, some engineering and environmental problems related to the mechanical properties of gas hydrate-bearing sediments (GHBS) during gas recovery may occur. Many studies have been carried out on the basic mechanical properties of GHBS samples based on laboratory tests, but their evolution characteristics and suitable models require further research. Based on a series of data analyses of published laboratory experimental results on GHBS samples with different hydrate saturations under various confining pressures, the evolution characteristics of strength and dilation parameters were investigated. It was found that cohesion (c) increases quickly to a peak value and then decreases gradually to a residual value with an increasing plastic shear strain, and the samples with higher hydrate saturations have higher initial values, peak values, and residual values of cohesion (c). The internal friction angle (φ) increases quickly with increasing plastic shear strain and then becomes stable at a residual value for all the samples with different hydrate saturations. The dilation angle (ψ) increases from negative to positive values with increasing plastic shear strain and then becomes stable at a residual value. These characteristics are likely to be related to the compaction occurring at the early stage of compression before expansion due to dilation. In this paper, a non-linearly fitted model is proposed considering the evolution of the mechanical parameters, and the verification tests show that the proposed model can simulate the stress–strain behaviors of the GHBS samples well. This model is also adopted in the stability analysis of submarine slopes containing hydrate reservoirs. The analytical approach is developed, accompanied by the strength reduction method.
Dynamic Deflection Measurement on Stiff Bridges with High Piers by Preloaded Spring Method
Authors: Wang Yelu , Zhou Yongjun , Jiang Xin , Zhao Yu , Zhang Huantao
Published: March 2024

The deflection dynamic load allowance (DLA) of stiff bridges with high piers requires sub-millimeter accuracy. New technologies such as the vision-based optical method and GNSS are not yet recognized for use in DLA measurements due to their smaller SNR. Presently, the scaffolding method is widely utilized for dynamic deflection measurements in dynamic load tests owing to the reliability of employing rigid contact. When scaffolding is not available, engineers have to resort to a suspension hammer system. However, the mass eccentricity of the hammer, stretched-wire length, and wind will decrease the measurement accuracy. To overcome these drawbacks of the suspension hammer method (SHM), a preloaded spring method (PSM) and the related stretched-wire-spring system (SWSS) are proposed in this paper. The dynamic deflection of the coupled vehicle-bridge-SWSS was obtained by vehicle-bridge interaction (VBI) analysis. The sensitivity parameters of the PSM were analyzed and optimized to minimize the measurement error. Indoor experiments and field dynamic load tests were conducted to validate the feasibility and accuracy of the PSM. Additionally, the differences in dynamic deflection measurements between the PSM and SHM in windy environments were compared. The results show that, in a windless environment, the DLAs of the PSM are affected by the spring stiffness, stretched-wire length, and stretched-wire section stiffness, independently of the preload force. When the wind speed is less than or equal to 8 m/s and the pier height is less than 30 m, the maximum deflection measurement error of the PSM is −2.53%, while that of the SHM is −15.87%. Due to its low cost and high accuracy, the proposed method has broad application prospects in the dynamic deflection measurement of stiff bridges with high piers.
Numerical Investigation of the R-Curve Effect in Delamination of Composite Materials Using Cohesive Elements
Authors: Raimondo Antonio
Published: March 2024

This paper presents a numerical investigation of the R-curve effect in delamination propagation in composite materials. The R-curve effect refers to the phenomenon whereby resistance to crack propagation increases with the advancement of the delamination, due to toughening mechanisms, such as fiber bridging. Numerical models often neglect this effect assuming a constant value of the fracture toughness. A numerical approach based on cohesive elements and on the superposition of two bilinear traction-separation laws is adopted here to accurately predict the R-curve effect in skin-doubler composite specimens subjected to three-point bending tests. The carbon-epoxy material presents two different sensitivities to the fiber bridging phenomenon resulting in two different R-curves. Comparisons with literature experimental data, in terms of load and delaminated area vs. applied displacement, and ultrasonic C-scan images show the effectiveness of the adopted approach in simulating the R-curve effect. The predicted numerical stiffness aligns with the experimental scatter, although the maximum load is slightly underestimated by approximately 15% compared with the average experimental results. The numerical model accurately predict the R-curve effect observed in the experimental data, demonstrating a 31% increase in the maximum load for the material configuration exhibiting greater sensitivity to fiber bridging.
Industry 4.0 and Smart Systems in Manufacturing: Guidelines for the Implementation of a Smart Statistical Process Control
Authors: Goecks Lucas Schmidt , Habekost Anderson Felipe , Coruzzolo Antonio Maria , Sellitto Miguel Afonso
Published: March 2024

Digital transformations in manufacturing systems confer advantages for enhancing competitiveness and ensuring the survival of companies by reducing operating costs, improving quality, and fostering innovation, falling within the overarching umbrella of Industry 4.0. This study aims to provide a framework for the integration of smart statistical digital systems into existing manufacturing control systems, exemplified with guidelines to transform an existent statistical process control into a smart statistical process control. Employing the design science research method, the research techniques include a literature review and interviews with experts who critically evaluated the proposed framework. The primary contribution lies in a set of general-purpose guidelines tailored to assist practitioners in manufacturing systems with the implementation of digital, smart technologies aligned with the principles of Industry 4.0. The resulting guidelines specifically target existing manufacturing plants seeking to adopt new technologies to maintain competitiveness. The main implication of the study is that practitioners can utilize the guidelines as a roadmap for the ongoing development and implementation of project management. Furthermore, the study paves the way for open innovation initiatives by breaking down the project into defined steps and encouraging individual or collective open contributions, which consolidates the practice of open innovation in manufacturing systems.
Assessment of Water Resources under Climate Change in Western Hindukush Region: A Case Study of the Upper Kabul River Basin
Authors: Ayoubi Tooryalay , Reinhardt-Imjela Christian , Schulte Achim
Published: March 2024

This study aims to estimate the surface runoff and examine the impact of climate change on water resources in the Upper Kabul River Basin (UKRB). A hydrological model was developed using the Soil and Water Assessment Tool (SWAT) from 2009 to 2019. The monthly calibration was conducted on streamflow in six stations for the period from 2010 to 2016, and the results were validated from 2017 to 2018 based on available observed data. The hydrological sensitivity parameters were further prioritized using SWAT-CUP. The uncertainty of the model was analyzed by the 95% Prediction Uncertainty (95PPU). Future projections were analyzed for the 2040s (2030–2049) and 2090s (2080–2099) compared to the baseline period (1986–2005) under two representation concentration pathways (RCP4.5, RCP8.5). Four Regional Climate Models (RCMs) were bias-corrected using the linear scaling bias correction method. The modeling results exhibited a very reasonable fit between the estimated and observed runoff in different stations, with NS values ranging from 0.54 to 0.91 in the calibration period. The future mean annual surface runoff exhibited an increase in the 2040s and 2090s compared to the baseline under both RCPs of 4.5 and 8.5 due to an increase in annual precipitation. The annual precipitation is projected to increase by 5% in the 2040s, 1% in the 2090s under RCP4.5, and by 9% in the 2040s and 2% in the 2090s under RCP8.5. The future temperature is also projected to increase and consequently lead to earlier snowmelt, resulting in a shift in the seasonal runoff peak to earlier months in the UKRB. However, the shifts in the timing of runoff could lead to significant impacts on water availability and exacerbate the water stress in this region, decreasing in summer runoff and increasing in the winter and spring runoffs. The future annual evapotranspiration is projected to increase under both scenarios; however, decreases in annual snowfall, snowmelt, sublimation, and groundwater recharge are predicted in the UKRB.
Associations between Climate Variability and Livestock Production in Botswana: A Vector Autoregression with Exogenous Variables (VARX) Analysis
Authors: Matopote Given , Joshi Niraj Prakash
Published: March 2024

The changing climate has a serious bearing on agriculture, particularly livestock production in Botswana. Therefore, studying the relationship between climate and livestock, which at present is largely missing, is necessary for the proper formulation of government policy and interventions. This is critical in promoting the adoption of relevant mitigation strategies by farmers, thereby increasing resilience. The aim of this research is to establish associations between climate variability and livestock production in Botswana at the national level. The paper employs time series data from 1970 to 2020 and the Vector Autoregression with Exogenous Variables (VARX) model for statistical analysis. The trend shows that both cattle and goat populations are decreasing. The VARX model results reveal that cattle and goat populations are negatively associated with increasing maximum temperatures. Cattle respond negatively to increased minimum temperatures as well, while goats tend to respond positively, implying that livestock species react differently to climatic conditions due to their distinct features. The results of the roots of the companion matrix for cattle and goat production meet the stability condition as all the eigenvalues lie inside the unit circle. The study recommends further intervention by the government to deal with increasing temperatures, thereby addressing the dwindling populations of goats and cattle, which have significant contributions to the household economies of smallholders and the national economy, respectively.
Estimation and Analysis of Glacier Mass Balance in the Southeastern Tibetan Plateau Using TanDEM-X Bi-Static InSAR during 2000–2014
Authors: Sun Yafei , Jiang Liming , Gao Ning , Gao Songfeng , Li Junjie
Published: March 2024

In recent decades, glaciers in the southeastern Tibetan Plateau (SETP) have been rapidly melting and showing a large scale of glacier mass loss. Due to the lack of large-scale, high-resolution, and high-precision observations, knowledge on the spatial distribution of the glacier mass balance and the response to climate change is limited in this region. We propose a TanDEM-X bi-static InSAR (Interferometric Synthetic Aperture Radar) algorithm with a non-local mean filter method and difference strategy, to improve the precision of glacier surface elevation change detection. Moreover, we improved the glacier mass balance estimation algorithm with a correction method for multi-source system errors and an uncertainty evaluation method based on error propagation theory to reduce the uncertainty of estimations. We used 13 pairs of TanDEM-X bi-static InSAR images to obtain the glacier mass balance data for the entire SETP. The total area of glaciers monitored was 5821 km2 and the total number of glaciers monitored was 2321; the glacier surface elevation change rate was −0.505 ± 0.005 m/yr, and the glacier mass balance estimation was −454.5 ± 13.1 mm w.eq. during 2000–2014. Additionally, we analyzed the spatial distribution of the glacier mass balance within the SETP using the sub-watershed analysis method. The results showed that the mass loss rate had a decreasing trend from the southeast to the northwest. Furthermore, the temperature change and the glacier mass loss rate showed a positive correlation from the southeast to the northwest in this region. This study greatly advances our understanding of the regularities of glacier dynamics in this region, and can provide scientific support for major national goals such as the rational utilization of surrounding water resources and construction of important transportation projects.
Generating Geometric Patterns Using Complex Polynomials and Iterative Schemes
Authors: Tassaddiq Asifa , Kalsoom Amna , Rashid Maliha , Sehr Kainat , Almutairi Dalal Khalid
Published: March 2024

Iterative procedures have been proved as a milestone in the generation of fractals. This paper presents a novel approach for generating and visualizing fractals, specifically Mandelbrot and Julia sets, by utilizing complex polynomials of the form QC(p)=apn+mp+c, where n≥2. It establishes escape criteria that play a vital role in generating these sets and provides escape time results using different iterative schemes. In addition, the study includes the visualization of graphical images of Julia and Mandelbrot sets, revealing distinct patterns. Furthermore, the study also explores the impact of parameters on the deviation of dynamics, color, and appearance of fractals.
The Nitrogen Content in the Fruiting Body and Mycelium of Pleurotus Ostreatus and Its Utilization as a Medium Component in Thraustochytrid Fermentation
Authors: Schütte Lina , Hausmann Katharina , Schwarz Christoph , Ersoy Franziska , Berger Ralf G.
Published: March 2024

Following the idea of a circular bioeconomy, the use of side streams as substitutes for cultivation media (components) in bioprocesses would mean an enormous economic and ecological advantage. Costly compounds in conventional media for the production of the triterpene squalene in thraustochytrids are the main carbon source and complex nitrogen sources. Among other side streams examined, extracts from the spent mycelium of the basidiomycete Pleurotus ostreatus were best-suited to acting as alternative nitrogen sources in cultivation media for thraustochytrids. The total nitrogen (3.76 ± 0.01 and 4.24 ± 0.04%, respectively) and protein (16.47 ± 0.06 and 18.57 ± 0.18%, respectively) contents of the fruiting body and mycelium were determined. The fungal cells were hydrolyzed and extracted to generate accessible nitrogen sources. Under preferred conditions, the extracts from the fruiting body and mycelium contained 73.63 ± 1.19 and 89.93 ± 7.54 mM of free amino groups, respectively. Cultivations of Schizochytrium sp. S31 on a medium using a mycelium extract as a complex nitrogen source showed decelerated growth but a similar squalene yield (123.79 ± 14.11 mg/L after 216 h) compared to a conventional medium (111.29 ± 19.96 mg/L, although improvable by additional complex nitrogen source).
PTCH1 Gene Variants, mRNA Expression, and Bioinformatics Insights in Mexican Cutaneous Squamous Cell Carcinoma Patients
Authors: Zambrano-Román Marianela , Padilla-Gutiérrez Jorge R. , Valle Yeminia , Muñoz-Valle José Francisco , Guevara-Gutiérrez Elizabeth , López-Olmos Patricia Aidé , Sepúlveda-Loza Laura Cristina , Bautista-Herrera Luis Alberto , Valdés-Alvarado Emmanuel
Published: March 2024

Background: Skin cancer is one of the most frequent types of cancer, and cutaneous squamous cell carcinoma (cSCC) constitutes 20% of non-melanoma skin cancer (NMSC) cases. PTCH1, a tumor suppressor gene involved in the Sonic hedgehog signaling pathway, plays a crucial role in neoplastic processes. Methods: An analytical cross-sectional study, encompassing 211 cSCC patients and 290 individuals in a control group (CG), was performed. A subgroup of samples was considered for the relative expression analysis, and the results were obtained using quantitative real-time PCR (qPCR) with TaqMan® probes. The functional, splicing, and disease-causing effects of the proposed variants were explored via bioinformatics. Results: cSCC was predominant in men, especially in sun-exposed areas such as the head and neck. No statistically significant differences were found regarding the rs357564, rs2236405, rs2297086, and rs41313327 variants of PTCH1, or in the risk of cSCC, nor in the mRNA expression between the cSCC group and CG. A functional effect of rs357564 and a disease-causing relation to rs41313327 was identified. Conclusion: The proposed variants were not associated with cSCC risk in this Mexican population, but we recognize the need for analyzing larger population groups to elucidate the disease-causing role of rare variants.
Reducing Epistasis and Pleiotropy Can Avoid the Survival of the Flattest Tragedy
Authors: Mehra Priyanka , Hintze Arend
Published: March 2024

This study investigates whether reducing epistasis and pleiotropy enhances mutational robustness in evolutionary adaptation, utilizing an indirect encoded model within the “survival of the flattest” (SoF) fitness landscape. By simulating genetic variations and their phenotypic consequences, we explore organisms’ adaptive mechanisms to maintain positions on higher, narrower evolutionary peaks amidst environmental and genetic pressures. Our results reveal that organisms can indeed sustain their advantageous positions by minimizing the complexity of genetic interactions—specifically, by reducing the levels of epistasis and pleiotropy. This finding suggests a counterintuitive strategy for evolutionary stability: simpler genetic architectures, characterized by fewer gene interactions and multifunctional genes, confer a survival advantage by enhancing mutational robustness. This study contributes to our understanding of the genetic underpinnings of adaptability and robustness, challenging traditional views that equate complexity with fitness in dynamic environments.
Exploring SVA Insertion Polymorphisms in Shaping Differential Gene Expressions in the Central Nervous System
Authors: Hughes Lauren S. , Fröhlich Alexander , Pfaff Abigail L. , Bubb Vivien J. , Quinn John P. , Kõks Sulev
Published: March 2024

Transposable elements (TEs) are repetitive elements which make up around 45% of the human genome. A class of TEs, known as SINE-VNTR-Alu (SVA), demonstrate the capacity to mobilise throughout the genome, resulting in SVA polymorphisms for their presence or absence within the population. Although studies have previously highlighted the involvement of TEs within neurodegenerative diseases, such as Parkinson’s disease and amyotrophic lateral sclerosis (ALS), the exact mechanism has yet to be identified. In this study, we used whole-genome sequencing and RNA sequencing data of ALS patients and healthy controls from the New York Genome Centre ALS Consortium to elucidate the influence of reference SVA elements on gene expressions genome-wide within central nervous system (CNS) tissues. To investigate this, we applied a matrix expression quantitative trait loci analysis and demonstrate that reference SVA insertion polymorphisms can significantly modulate the expression of numerous genes, preferentially in the trans position and in a tissue-specific manner. We also highlight that SVAs significantly regulate mitochondrial genes as well as genes within the HLA and MAPT loci, previously associated within neurodegenerative diseases. In conclusion, this study continues to bring to light the effects of polymorphic SVAs on gene regulation and further highlights the importance of TEs within disease pathology.
CD69 Signaling in Eosinophils Induces IL-10 Production and Apoptosis via the Erk1/2 and JNK Pathways, Respectively
Authors: Bui Dan Van , Nguyen Linh Manh , Kanda Akira , Chu Hanh Hong , Thi Le Nhi Kieu , Yun Yasutaka , Kobayashi Yoshiki , Suzuki Kensuke , Mitani Akitoshi , Shimamura Akihiro , Fukui Kenta , Sawada Shunsuke , Dombrowicz David , Iwai Hiroshi
Published: March 2024

Introduction: Eosinophils contribute to the pathogenesis of allergic diseases, including asthma, allergic rhinitis, and atopic dermatitis. We previously reported that human tissue eosinophils have high CD69 expression compared to blood eosinophils, and its expression is correlated with disease severity and the number of infiltrated eosinophils. However, biological CD69 signaling activity in eosinophils remains unclear. Methods: CD69 expression on lung tissue eosinophils obtained from mice with ovalbumin-induced asthma was measured using flow cytometry. CD69 crosslinking was performed on eosinophils purified from the spleen of IL-5 transgenic mice to investigate CD69 signaling and its function in eosinophils. Then, qPCR, Western blot, enzyme-linked immunosorbent assay, and survival assay results were analyzed. Results: Surface CD69 expression on lung tissue eosinophils in the asthma mice model was 2.91% ± 0.76%, whereas no expression was detected in the healthy group. CD69-expressed eosinophils intrinsically have an upregulation of IL-10 mRNA expression. Moreover, CD69 crosslinking induced further pronounced IL-10 production and apoptosis; these responses were mediated via the Erk1/2 and JNK pathways, respectively. Conclusions: Our results suggested that CD69+ eosinophils play an immunoregulator role in type 2 inflammation, whereas activated tissue eosinophils contribute to the pathogenesis of asthma.
Lipid Rafts: The Maestros of Normal Brain Development
Authors: Viljetić Barbara , Blažetić Senka , Labak Irena , Ivić Vedrana , Zjalić Milorad , Heffer Marija , Balog Marta
Published: March 2024

Lipid rafts, specialised microdomains within cell membranes, play a central role in orchestrating various aspects of neurodevelopment, ranging from neural differentiation to the formation of functional neuronal networks. This review focuses on the multifaceted involvement of lipid rafts in key neurodevelopmental processes, including neural differentiation, synaptogenesis and myelination. Through the spatial organisation of signalling components, lipid rafts facilitate precise signalling events that determine neural fate during embryonic development and in adulthood. The evolutionary conservation of lipid rafts underscores their fundamental importance for the structural and functional complexity of the nervous system in all species. Furthermore, there is increasing evidence that environmental factors can modulate the composition and function of lipid rafts and influence neurodevelopmental processes. Understanding the intricate interplay between lipid rafts and neurodevelopment not only sheds light on the fundamental mechanisms governing brain development but also has implications for therapeutic strategies aimed at cultivating neuronal networks and addressing neurodevelopmental disorders.
Continuous and Non-Invasive Lactate Monitoring Techniques in Critical Care Patients
Authors: Lafuente Jose-Luis , González Samuel , Aibar Clara , Rivera Desirée , Avilés Eva , Beunza Juan-Jose
Published: March 2024

Lactate, once merely regarded as an indicator of tissue hypoxia and muscular fatigue, has now gained prominence as a pivotal biomarker across various medical disciplines. Recent research has unveiled its critical role as a high-value prognostic marker in critical care medicine. The current practice of lactate detection involves periodic blood sampling. This approach is invasive and confined to measurements at six-hour intervals, leading to resource expenditure, time consumption, and patient discomfort. This review addresses non-invasive sensors that enable continuous monitoring of lactate in critical care patients. After the introduction, it discusses the iontophoresis system, followed by a description of the structural materials that are universally employed to create an interface between the integumentary system and the sensor. Subsequently, each method is detailed according to its physical principle, outlining its advantages, limitations, and pertinent aspects. The study concludes with a discussion and conclusions, aiming at the design of an intelligent sensor (Internet of Medical Things or IoMT) to facilitate continuous lactate monitoring and enhance the clinical decision-making support system in critical care medicine.
The Mediating Effect of Depression on the Relationship between Loneliness and Substance Use in Korean Adolescents
Authors: Kim Hyesun
Published: March 2024

Substance use among adolescents is a major emerging health problem worldwide. Although loneliness and depression are major risk factors for substance use, few studies have examined the relationship between loneliness, depression, and substance use in adolescents. This study aimed to determine the mediating effect of depression on the relationship between loneliness and substance use among Korean adolescents, based on the data from 53,310 adolescents from the 17th Korea Youth Risk Behavior Web-based Survey in 2021. Using a complex sample analysis module, hierarchical logistic regression analysis was employed to confirm the mediating effect of depression on the relationship between loneliness and substance use. The results showed that loneliness and depression have a significant effect on substance use (smoking, drinking alcohol, and drug use). Depression was found to have a partial mediating effect on the relationship between loneliness and substance use. Overall, the results suggested that loneliness and depression in adolescents increase substance use, and loneliness can affect substance use through depression. Therefore, proactive strategies to prevent and reduce loneliness and depression in adolescents can be effective in preventing substance use.
The Mediating Role of Emotional Arousal and Donation Anxiety on Blood Donation Intentions: Expanding on the Theory of Planned Behavior
Authors: Balaskas Stefanos , Koutroumani Maria , Rigou Maria
Published: March 2024

Blood donation is essential in health-care systems worldwide, dealing with the demand for transfusions, and for the treatment of a variety of medical conditions. A major obstacle is raising the rate of blood donations by recruiting and retaining donors in an efficient manner. This paper presents a comprehensive analysis of blood donation, utilizing an enhanced framework based on the theory of planned behavior with an emphasis on emotional arousal (positive and negative), attitudes towards advertisements, and blood donation anxiety, revealing critical psychological and communicative determinants of blood donation intention. To achieve this, a quantitative non-experimental correlational technique was employed to collect data from 414 individuals using an online questionnaire circulated across Greek society. The data were analyzed using structural equation modelling, with a focus on the direct impacts on donation intentions and the role of emotional arousal as a mediator. The findings indicate that attitudes and anxiety have strong direct impacts on the behavioral intention to donate, underlining the important barriers generated by donation anxieties as well as the efficacy of positive attitudes and successful advertising. Furthermore, the study demonstrates emotional arousal as a partial mediator, implying that both cognitive assessments and emotional responses play a role in influencing donation intentions. This study takes on a new approach to give emphasis and provide evidence of the mediating effect of emotional arousal on donation intention, utilizing structural equation modeling. Despite the critical role of marketing as a primary source of blood donors, the implementation of emotional marketing techniques has been one aspect less addressed throughout marketing professionals and communication efforts. Our results demonstrate the significance of emotional arousal on blood donation intentions, thus suggesting a more emotionally resonant approach of attracting potential donors.