bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of a Multistarter Yeast Inoculum on Ethanol Reduction and Population Dynamics in Wine Fermentation
Authors: Zhu Xiaolin , Torija María-Jesús , Mas Albert , Beltran Gemma , Navarro Yurena
Published: March 2021

Microbiological strategies are currently being considered as methods for reducing the ethanol content of wine. Fermentations started with a multistarter of three non-Saccharomyces yeasts (Metschnikowia pulcherrima (Mp), Torulaspora delbrueckii (Td) and Zygosaccharomyces bailii (Zb)) at different inoculum concentrations. S. cerevisiae (Sc) was inoculated into fermentations at 0 h (coinoculation), 48 h or 72 h (sequential fermentations). The microbial populations were analyzed by a culture-dependent approach (Wallerstein Laboratory Nutrient (WLN) culture medium) and a culture-independent method (PMA-qPCR). The results showed that among these three non-Saccharomyces yeasts, Td became the dominant non-Saccharomyces yeast in all fermentations, and Mp was the minority yeast. Sc was able to grow in all fermentations where it was involved, being the dominant yeast at the end of fermentation. We obtained a significant ethanol reduction of 0.48 to 0.77% (v/v) in sequential fermentations, with increased concentrations of lactic and acetic acids. The highest reduction was achieved when the inoculum concentration of non-Saccharomyces yeast was 10 times higher (107 cells/mL) than that of S. cerevisiae. However, this reduction was lower than that obtained when these strains were used as single non-Saccharomyces species in the starter, indicating that interactions between them affected their performance. Therefore, more combinations of yeast species should be tested to achieve greater ethanol reductions.
Proposed Model for Shale Compaction Kinetics
Authors: Smith James Edward , Smith-Rowland Edward Millard
Published: March 2021

Shales are the most abundant class of sedimentary rocks, distinguished by being very fine-grained, clayey, and compressible. Their physical and chemical properties are important in widely different enterprises such as civil engineering, ceramics, and petroleum exploration. One characteristic, which is studied here, is a systematic reduction of porosity with depth of burial. This is due increases in grain-to-grain stress and temperature. Vertical stress in sediments is given by the overburden less the pore fluid pressure, σ, divided by the fraction of the horizontal area which is the supporting matrix, (1−φ), where φ is the porosity. It is proposed that the fractional reduction of this ratio, Λ, with time is given by the product of φ4m/3, (1−φ)4n/3, and one or more Arrhenius functions Aexp(−E/RT) with m and n close to 1. This proposal is tested for shale sections in six wells from around the world for which porosity-depth data are available. Good agreement is obtained above 30–40 °C and fractional porosities less than 0.5. Single activation energies for each well are obtained in the range 15–33 kJ/mole, close to the approximate pressure solution of quartz, 24 kJ/mol. Values of m and n are in the range 1 to 0.8, indicating nearly fractal water-wet pore-to-matrix interfaces at pressure solution locations. Results are independent of over- or under-pressure of pore water. This model attempts to explain shale compaction quantitatively. For the petoleum industry, given porosity-depth data for uneroded sections and accurate activation energy, E, paleo-geothermal-gradient can be inferred and from that organic maturity, indicating better drilling prospects.
Modified Deep Reinforcement Learning with Efficient Convolution Feature for Small Target Detection in VHR Remote Sensing Imagery
Authors: Liu Shuai , Tang Jialan
Published: March 2021

Small object detection in very-high-resolution (VHR) optical remote sensing images is a fundamental but challenaging problem due to the latent complexities. To tackle this problem, the MdrlEcf model is proposed by modifying deep reinforcement learning (DRL) and extracting the efficient convolution feature. Firstly, an efficient attention network is constructed by introducing the local attention into the convolutional neural network. Combining the shallow low-level features with rich detail descriptions and high-level features with more semantic meanings effectively, efficient convolution features can be obtained. By this, the attention network can effectively enhance the ability to extract small target features and suppressing useless features. Secondly, the efficient feature map is sent to the region proposal network constructed by modified DRL. Using the modified reward function, this model can accumulate more rewards to conduct the search process, and potentially generate effective subsequent proposals and classification scores. It also can increase the effectiveness of object locations and classifications for small targets. Quantitative and qualitative experiments are conducted to verify the detection performance of different models. The results show that the proposed MdrlEcf can effectively and accurately locate and identify related small objects.
Coupling Degree between the Demand and Supply of Bus Services at Stops: A Density-Based Approach
Authors: Li Bowen , Huang Zhengdong , Xia Jizhe , Li Wenshu , Zhang Ying
Published: March 2021

The bus stop layout and route deployment may influence the efficiency of bus services. Evaluating the supply of bus service requires the consideration of demand from various urban activities, such as residential and job-related activities. Although various evaluation methods have been explored from different perspectives, it remains a challenging issue. This study proposes a spatial statistical approach by comparing the density of the potential demand and supply of bus services at bus stops. The potential demand takes jobs-housing locations into account, and the supply of bus services considers bus stops and their associated total number of daily bus arrivals. The kernel density estimation (KDE) and spatial autocorrelation analyses are employed to investigate the coupling relationship between the demand and supply densities at global and local scales. A coupling degree index (CDI) is constructed to standardize the measurement of demand-supply balance. A case study in Wuhan, China demonstrated that: (1) the spatial distribution of bus stops is reasonable at global level, (2) Seriously unbalanced locations for bus services have been detected at several stops. Related adjustments that can improve these defects are highly recommended.
Reduced Granule Cell Proliferation and Molecular Dysregulation in the Cerebellum of Lysosomal Acid Phosphatase 2 (ACP2) Mutant Mice
Authors: Jiao Xiaodan , Rahimi Balaei Maryam , Abu-El-Rub Ejlal , Casoni Filippo , Pezeshgi Modarres Hassan , Dhingra Sanjiv , Kong Jiming , Consalez Giacomo G. , Marzban Hassan
Published: March 2021

Lysosomal acid phosphatase 2 (Acp2) mutant mice (naked-ataxia, nax) have a severe cerebellar cortex defect with a striking reduction in the number of granule cells. Using a combination of in vivo and in vitro immunohistochemistry, Western blotting, BrdU assays, and RT-qPCR, we show downregulation of MYCN and dysregulation of the SHH signaling pathway in the nax cerebellum. MYCN protein expression is significantly reduced at P10, but not at the peak of proliferation at around P6 when the number of granule cells is strikingly reduced in the nax cerebellum. Despite the significant role of the SHH–MycN pathway in granule cell proliferation, our study suggests that a broader molecular pathway and additional mechanisms regulating granule cell development during the clonal expansion period are impaired in the nax cerebellum. In particular, our results indicate that downregulation of the protein synthesis machinery may contribute to the reduced number of granule cells in the nax cerebellum.
Ascorbic Acid Supplementation Improves Skeletal Muscle Growth in Pacu (Piaractus mesopotamicus) Juveniles: In Vivo and In Vitro Studies
Authors: Zanella Bruna Tereza Thomazini , Magiore Isabele Cristina , Duran Bruno Oliveira Silva , Pereira Guilherme Gutierrez , Vicente Igor Simões Tiagua , Carvalho Pedro Luiz Pucci Figueiredo , Salomão Rondinelle Artur Simões , Mareco Edson Assunção , Carvalho Robson Francisco , Paula Tassiana Gutierrez de , Barros Margarida Maria , Dal-Pai-Silva Maeli
Published: March 2021

In fish, fasting leads to loss of muscle mass. This condition triggers oxidative stress, and therefore, antioxidants can be an alternative to muscle recovery. We investigated the effects of antioxidant ascorbic acid (AA) on the morphology, antioxidant enzyme activity, and gene expression in the skeletal muscle of pacu (Piaractus mesopotamicus) following fasting, using in vitro and in vivo strategies. Isolated muscle cells of the pacu were subjected to 72 h of nutrient restriction, followed by 24 h of incubation with nutrients or nutrients and AA (200 µM). Fish were fasted for 15 days, followed by 6 h and 15 and 30 days of refeeding with 100, 200, and 400 mg/kg of AA supplementation. AA addition increased cell diameter and the expression of anabolic and cell proliferation genes in vitro. In vivo, 400 mg/kg of AA increased anabolic and proliferative genes expression at 6 h of refeeding, the fiber diameter and the expression of genes related to cell proliferation at 15 days, and the expression of catabolic and oxidative metabolism genes at 30 days. Catalase activity remained low in the higher supplementation group. In conclusion, AA directly affected the isolated muscle cells, and the higher AA supplementation positively influenced muscle growth after fasting.
Endothelial Glycocalyx as a Regulator of Fibrotic Processes
Authors: Masola Valentina , Zaza Gianluigi , Arduini Arduino , Onisto Maurizio , Gambaro Giovanni
Published: March 2021

The endothelial glycocalyx, the gel layer covering the endothelium, is composed of glycosaminoglycans, proteoglycans, and adsorbed plasma proteins. This structure modulates vessels’ mechanotransduction, vascular permeability, and leukocyte adhesion. Thus, it regulates several physiological and pathological events. In the present review, we described the mechanisms that disturb glycocalyx stability such as reactive oxygen species, matrix metalloproteinases, and heparanase. We then focused our attention on the role of glycocalyx degradation in the induction of profibrotic events and on the possible pharmacological strategies to preserve this delicate structure.
Screening of 5- and 6-Substituted Amiloride Libraries Identifies Dual-uPA/NHE1 Active and Single Target-Selective Inhibitors
Authors: Buckley Benjamin J. , Kumar Ashna , Aboelela Ashraf , Bujaroski Richard S. , Li Xiuju , Majed Hiwa , Fliegel Larry , Ranson Marie , Kelso Michael J.
Published: March 2021

The K+-sparing diuretic amiloride shows off-target anti-cancer effects in multiple rodent models. These effects arise from the inhibition of two distinct cancer targets: the trypsin-like serine protease urokinase-type plasminogen activator (uPA), a cell-surface mediator of matrix degradation and tumor cell invasiveness, and the sodium-hydrogen exchanger isoform-1 (NHE1), a central regulator of transmembrane pH that supports carcinogenic progression. In this study, we co-screened our library of 5- and 6-substituted amilorides against these two targets, aiming to identify single-target selective and dual-targeting inhibitors for use as complementary pharmacological probes. Closely related analogs substituted at the 6-position with pyrimidines were identified as dual-targeting (pyrimidine 24 uPA IC50 = 175 nM, NHE1 IC50 = 266 nM, uPA selectivity ratio = 1.5) and uPA-selective (methoxypyrimidine 26 uPA IC50 = 86 nM, NHE1 IC50 = 12,290 nM, uPA selectivity ratio = 143) inhibitors, while high NHE1 potency and selectivity was seen with 5-morpholino (29 NHE1 IC50 = 129 nM, uPA IC50 = 10,949 nM; NHE1 selectivity ratio = 85) and 5-(1,4-oxazepine) (30 NHE1 IC50 = 85 nM, uPA IC50 = 5715 nM; NHE1 selectivity ratio = 67) analogs. Together, these amilorides comprise a new toolkit of chemotype-matched, non-cytotoxic probes for dissecting the pharmacological effects of selective uPA and NHE1 inhibition versus dual-uPA/NHE1 inhibition.
Is High-Intensity Interval Training Suitable to Promote Neuroplasticity and Cognitive Functions after Stroke?
Authors: Hugues Nicolas , Pellegrino Christophe , Rivera Claudio , Berton Eric , Pin-Barre Caroline , Laurin Jérôme
Published: March 2021

Stroke-induced cognitive impairments affect the long-term quality of life. High-intensity interval training (HIIT) is now considered a promising strategy to enhance cognitive functions. This review is designed to examine the role of HIIT in promoting neuroplasticity processes and/or cognitive functions after stroke. The various methodological limitations related to the clinical relevance of studies on the exercise recommendations in individuals with stroke are first discussed. Then, the relevance of HIIT in improving neurotrophic factors expression, neurogenesis and synaptic plasticity is debated in both stroke and healthy individuals (humans and rodents). Moreover, HIIT may have a preventive role on stroke severity, as found in rodents. The potential role of HIIT in stroke rehabilitation is reinforced by findings showing its powerful neurogenic effect that might potentiate cognitive benefits induced by cognitive tasks. In addition, the clinical role of neuroplasticity observed in each hemisphere needs to be clarified by coupling more frequently to cellular/molecular measurements and behavioral testing.
Integration of Data from Liquid–Liquid Phase Separation Databases Highlights Concentration and Dosage Sensitivity of LLPS Drivers
Authors: Farahi Nazanin , Lazar Tamas , Wodak Shoshana J. , Tompa Peter , Pancsa Rita
Published: March 2021

Liquid–liquid phase separation (LLPS) is a molecular process that leads to the formation of membraneless organelles, representing functionally specialized liquid-like cellular condensates formed by proteins and nucleic acids. Integrating the data on LLPS-associated proteins from dedicated databases revealed only modest agreement between them and yielded a high-confidence dataset of 89 human LLPS drivers. Analysis of the supporting evidence for our dataset uncovered a systematic and potentially concerning difference between protein concentrations used in a good fraction of the in vitro LLPS experiments, a key parameter that governs the phase behavior, and the proteomics-derived cellular abundance levels of the corresponding proteins. Closer scrutiny of the underlying experimental data enabled us to offer a sound rationale for this systematic difference, which draws on our current understanding of the cellular organization of the proteome and the LLPS process. In support of this rationale, we find that genes coding for our human LLPS drivers tend to be dosage-sensitive, suggesting that their cellular availability is tightly regulated to preserve their functional role in direct or indirect relation to condensate formation. Our analysis offers guideposts for increasing agreement between in vitro and in vivo studies, probing the roles of proteins in LLPS.
Chromatography-Independent Fractionation and Newly Identified Molecular Features of the Adzuki Bean (Vigna angularis Willd.) β-vignin Protein
Authors: Philadelpho Biane , Souza Victória , Souza Fabiani , Santos Johnnie , Batista Fabiana , Silva Mariana , Capraro Jessica , De Benedetti Stefano , Heinzl Giuditta C. , Cilli Eduardo , Scarafoni Alessio , Magni Chiara , Ferreira Ederlan
Published: March 2021

Adzuki seed β-vignin, a vicilin-like globulin, has proven to exert various health-promoting biological activities, notably in cardiovascular health. A simple scalable enrichment procedure of this protein for further nutritional and functional studies is crucial. In this study, a simplified chromatography-independent protein fractionation procedure has been optimized and described. The electrophoretic analysis showed a high degree of homogeneity of β-vignin isolate. Furthermore, the molecular features of the purified protein were investigated. The adzuki bean β-vignin was found to have a native size of 146 kDa, and the molecular weight determined was consistent with a trimeric structure. These were identified in two main polypeptide chains (masses of 56–54 kDa) that are glycosylated polypeptides with metal binding capacity, and one minor polypeptide chain with a mass 37 kDa, wherein these features are absent. The in vitro analysis showed a high degree of digestibility of the protein (92%) and potential anti-inflammatory capacity. The results lay the basis not only for further investigation of the health-promoting properties of the adzuki bean β-vignin protein, but also for a possible application as nutraceutical molecule.
Molecular Mechanism and Pathogenesis of Sarcopenia: An Overview
Authors: Picca Anna , Calvani Riccardo
Published: March 2021

Sarcopenia involves a progressive age-related decline of skeletal muscle mass and strength/function [...]
Methodological Designs Applied in the Development of Computer-Based Training Programs for the Cognitive Rehabilitation in People with Mild Cognitive Impairment (MCI) and Mild Dementia. Systematic Review
Authors: Diaz Baquero Angie A. , Dröes Rose-Marie , Perea Bartolomé María V. , Irazoki Eider , Toribio-Guzmán José Miguel , Franco-Martín Manuel A. , van der Roest Henriëtte
Published: March 2021

In recent years, different computer-based cognitive training (CT) programs for people with dementia (PwD) have been developed following a psychosocial approach. Aim: This systematic review aims to identify the methodological designs applied in the development of computer-based training (CCT) programs for the rehabilitation of cognitive functioning in people with mild cognitive impairment (MCI) or mild dementia. Methods: A systematic review was conducted using the databases PubMed and PsycINFO. The search period was between 2000–2019. The study selection and data extraction processes were carried out by two independent reviewers. The protocol was registered in International Prospective Register of Systematic Reviews (PROSPERO) under registration number CRD42020159027. Results: Thirteen studies met the inclusion criteria. The most frequently used methodological design in the development of CCT programs for people with MCI or mild dementia was the user-centered design (UCD). This design involves an interactive system characterized by the inclusion of end users from the initial stages of its development, throughout the establishment of functional requirements, and in the evaluation of the program’s usability and user-experience (UX). Conclusion: UCD was the most used methodological design for the development of CCT programs although there was quite some variation in how this design was applied. Recommendations for future studies about the development of CCT programs for people with MCI and mild dementia are given. Central focus should be the inclusion and active participation of end users from the initial stages of development.
Role of Narrow Band Imaging Technology in the Diagnosis and Follow up of Laryngeal Lesions: Assessment of Diagnostic Accuracy and Reliability in a Large Patient Cohort
Authors: Galli Jacopo , Settimi Stefano , Mele Dario Antonio , Salvati Antonio , Schiavi Enrico , Parrilla Claudio , Paludetti Gaetano
Published: March 2021

Background: The aim of this study was to assess diagnostic accuracy and reliability of narrow band imaging (NBI) in the differential diagnosis of laryngeal premalignant lesion, early cancers and recurrences. Material and methods: We enrolled 231 patients who underwent endoscopic examination with white light endoscopy (WLE) + NBI and divided them into two groups, group A, without previous radiochemotherapy and group B, with previous radiochemotherapy. When indicated, we performed surgical biopsies to evaluate sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), accuracy and likelihood of endoscopic examination comparing WLE alone and WLE + NBI. Results: A positive NBI lesion, compared with a negative NBI lesion, had a 29.68 (group A) and 13.96 (group B) times higher probability to be histologically positive (i.e., confirmed) compared with WLE alone improving the diagnostic accuracy. In group A, the NBI mode showed excellent sensitivity (95.0%), which was higher than WLE 2 mode (77.5%). However, the greatest differences were recorded regarding specificity (96.8% vs. 40.6%). In group B, both NBI alone and WLE + NBI mode showed a 94.1% specificity compared with WLE alone, which had a maximum specificity of 85.3%. The mode comparison between NBI and WLE in both groups showed a statistically significant difference, with p-values <0.0001. Conclusions: NBI represents a reliable technology in challenging situations, especially in the context of post-radiotherapy or post-surgical mucosal changes showing a high NPV. NBI could reduce the number of unnecessary biopsies related to increased microvascular anomaly revelation, which could help to identify early-stage lesions suitable for minimally invasive surgery and, consequently, decrease hospital admissions.
Daily Sitting for Long Periods Increases the Odds for Subclinical Atheroma Plaques
Authors: Perez-Lasierra Jose Luis , Laclaustra Martin , Guallar-Castillón Pilar , Casasnovas Jose Antonio , Casajús Jose Antonio , Jarauta Estibaliz , Gonzalez-Agüero Alejandro , Moreno-Franco Belen
Published: March 2021

Sedentarism is a risk factor for cardiovascular disease (CVD), but currently it is not clear how a sedentary behavior such as long sitting time can affect atherosclerosis development. This study examined the relationship between sitting time and the prevalence of carotid and femoral subclinical atherosclerosis. A cross-sectional analysis based on a subsample of 2082 participants belonging to the Aragon Workers’ Health Study was carried out. Ultrasonography was used to assess the presence of plaques in carotid and femoral territories; the validated Spanish version of the questionnaire on the frequency of engaging in physical activity used in the Nurses’ Health Study and the Health Professionals’ was used to assess physical activity and sitting time; and demographic, anthropometric, and clinical data were obtained by trained personnel during the annual medical examination. Participants were categorized into <9 h/day and ≥9 h/day sitting time groups. After adjusting for several confounders, compared with participants that remain seated <9 h/day, those participants who remain seated ≥9 h/day had, respectively, OR = 1.25 (95%CI: 1.01, 1.55, p < 0.05) and OR = 1.38 (95%CI: 1.09, 1.74, p < 0.05) for carotid and any-territory plaque presence. Remaining seated ≥9 h/day is associated with higher odds for carotid and any-territory plaque presence independently of physical activity levels and other cardiovascular risk factors.
Physical Exercise Moderates the Effects of Disability on Depression in People with Multiple Sclerosis during the COVID-19 Outbreak
Authors: Carotenuto Antonio , Scandurra Cristiano , Costabile Teresa , Lavorgna Luigi , Borriello Giovanna , Moiola Lucia , Inglese Matilde , Trojsi Francesca , Petruzzo Martina , Ianniello Antonio , Nozzolillo Agostino , Cellerino Maria , Boffa Giacomo , Rosa Laura , Chiodi Alessandro , Servillo Giuseppe , Moccia Marcello , Bonavita Simona , Filippi Massimo , Petracca Maria , Brescia Morra Vincenzo , Lanzillo Roberta
Published: March 2021

Physical disability impacts psychosocial wellbeing in people with multiple sclerosis. However, the role of physical activity in this context is still debated. By taking advantage of a previous survey, conducted online from 22 April to 7 May 2020, we performed a post-hoc analysis with the aim to assess the associations between disability, physical exercise, and mental health in multiple sclerosis. We retrieved the following data: (i) sociodemographic information, (ii) changes in lifestyle (including exercise), (iii) physical disability, as measured with the Patient-Determined Disease Steps scale, and (iv) anxiety feelings and depressive symptoms assessed via the items included in the Quality of Life in Neurological Disorders measurement system. Examination of the interaction plot showed that the effect of disability on depression, but not on anxious symptoms, was significant for all levels of physical exercise (low: b = 1.22, 95% C.I. 0.85, 1.58, p < 0.001; moderate: b = 0.95, 95% C.I. 0.66, 1.24, p < 0.001; and high: b = 0.68, 95% C.I. 0.24, 1.13, p = 0.003). Based on these data, we can conclude that disability significantly impacted depression during the COVID-19 pandemic, with physical activity playing a moderating role. Our results suggest that favoring exercise in multiple sclerosis (MS) would ameliorate psychological wellbeing regardless of the level of physical disability.
Abductor Muscle Force after Straight-Stem Compared to Short-Stem Total Hip Arthroplasty through a Modified Direct Lateral Approach: Functional Assessment of 70 Consecutive Patients of a Randomized Controlled Clinical Trial
Authors: Fuchs Michael , Hein Marie-Anne , Faschingbauer Martin , Sgroi Mirco , Bieger Ralf , Reichel Heiko , Freitag Tobias
Published: March 2021

Because of preservation of proximal femoral bone stock and minimized soft tissue trauma, short-stem implants are becoming increasingly important in total hip arthroplasty (THA). The postulated advantage regarding the functional outcome has not been verified. We hypothesized an increased abductor muscle strength by the use of a short-stem design. Seventy consecutive patients of a randomized clinical trial were included. Of these, 67 patients met the inclusion criteria after 12 months. Thirty-five patients received a standard straight stem and 32 patients a short-stem femoral component. All surgeries were performed by a modified direct lateral approach. Isometric muscle strength of the hip abductors was evaluated preoperatively 3 and 12 months after surgery. Harris hip score (HHS) and Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) scores were evaluated. After three months, there were no differences between the two groups; the abductor force was comparable to the preoperative initial values. After 12 months, a significant increase in muscle strength for the short stem patient group compared to preoperative baseline values was measured (straight-stem THA, 0.09 Nm/kg ± 0.4, p = 0.32; short-stem THA, 0.2 Nm/kg ± 0.3, p = 0.004). Comparison of the 12-month postoperative total HHS and WOMAC revealed no significant differences between both groups. A significant increase in hip abductor muscle strength 12 months after short-stem THA compared to conventional-stem THA was observed.
Comparison of Specimen Geometries for Measuring Through-Thickness Tensile Mechanical Properties of Fibre-Reinforced Polymer Composites
Authors: Pemberton Rory , Crocker Louise , Poole Matthew , Shaw Richard , Gower Michael
Published: March 2021

Engineering design of fibre-reinforced polymer (FRP) composite components requires reliable methods for measuring out-of-plane mechanical properties in the through-thickness (T-T) material direction. Within this work, existing indirect and direct test methods and geometries for measuring T-T tensile properties have been evaluated through experimental testing and finite element analysis (FEA). Experimental testing showed variations, particularly in failure properties, for both indirect (failure strengths from 10–94 MPa) and direct (failure strengths from 48–62 MPa) geometries. Results were shown to be in good agreement with FEA, which also confirmed stress concentration factors. A linear relationship between the magnitude of stress concentration factors and experimentally determined T-T tensile failure strengths was observed for all but one of the direct geometries evaluated. Improved knowledge of stress concentration factors from this work should help instil confidence for industry to use T-T tensile properties determined from these methods.
Metabolism in the Zebrafish Retina
Authors: Jaroszynska Natalia , Harding Philippa , Moosajee Mariya
Published: March 2021

Retinal photoreceptors are amongst the most metabolically active cells in the body, consuming more glucose as a metabolic substrate than even the brain. This ensures that there is sufficient energy to establish and maintain photoreceptor functions during and after their differentiation. Such high dependence on glucose metabolism is conserved across vertebrates, including zebrafish from early larval through to adult retinal stages. As the zebrafish retina develops rapidly, reaching an adult-like structure by 72 hours post fertilisation, zebrafish larvae can be used to study metabolism not only during retinogenesis, but also in functionally mature retinae. The interplay between rod and cone photoreceptors and the neighbouring retinal pigment epithelium (RPE) cells establishes a metabolic ecosystem that provides essential control of their individual functions, overall maintaining healthy vision. The RPE facilitates efficient supply of glucose from the choroidal vasculature to the photoreceptors, which produce metabolic products that in turn fuel RPE metabolism. Many inherited retinal diseases (IRDs) result in photoreceptor degeneration, either directly arising from photoreceptor-specific mutations or secondary to RPE loss, leading to sight loss. Evidence from a number of vertebrate studies suggests that the imbalance of the metabolic ecosystem in the outer retina contributes to metabolic failure and disease pathogenesis. The use of larval zebrafish mutants with disease-specific mutations that mirror those seen in human patients allows us to uncover mechanisms of such dysregulation and disease pathology with progression from embryonic to adult stages, as well as providing a means of testing novel therapeutic approaches.
Kinematic Comparison between Medially Congruent and Posterior-Stabilized Third-Generation TKA Designs
Authors: Ghirardelli Stefano , Asay Jessica L. , Leonardi Erika A. , Amoroso Tommaso , Andriacchi Thomas P. , Indelli Pier Francesco
Published: March 2021

Background: This study compares knee kinematics in two groups of patients who have undergone primary total knee arthroplasty (TKA) using two different modern designs: medially congruent (MC) and posterior-stabilized (PS). The aim of the study is to demonstrate only minimal differences between the groups. Methods: Ten TKA patients (4 PS, 6 MC) with successful clinical outcomes were evaluated through 3D knee kinematics analysis performed using a multicamera optoelectronic system and a force platform. Extracted kinematic data included knee flexion angle at heel-strike (KFH), peak midstance knee flexion angle (MSKFA), maximum and minimum knee adduction angle (KAA), and knee rotational angle at heel-strike. Data were compared with a group of healthy controls. Results: There were no differences in preferred walking speed between MC and PS groups, but we found consistent differences in knee function. At heel-strike, the knee tended to be more flexed in the PS group compared to the MC group; the MSKFA tended to be higher in the PS group compared to the MC group. There was a significant fluctuation in KAA during the swing phase in the PS group compared to the MC group, PS patients showed a higher peak knee flexion moment compared to MC patients, and the PS group had significantly less peak internal rotation moments than the MC group. Conclusions: Modern, third-generation TKA designs failed to reproduce normal knee kinematics. MC knees tended to reproduce a more natural kinematic pattern at heel-strike and during axial rotation, while PS knees showed better kinematics during mid-flexion.
Preferential Treatment as a Tool for Managing the Coastal Area Sustainable Development: The Case of the Vladivostok Free Port
Authors: Fedorenko Roman Vladimirovich , Khmeleva Galina Anatolievna
Published: March 2021

With the adoption of the sustainable development goals (SDGs), the world has recognized the need to move to responsible governance in many areas of life, including seaports, which are at the forefront of economic activity and environmental safety. The present paper examines the challenges and opportunities associated with the implementation of sustainable development principles under the free port scheme. The authors analyzed the Russian Audit Chamber report on the activities of the free port of Vladivostok and compared it with the pioneer experience of the sustainable development of the port of Antwerp. The results show that focusing only on the economic and social objectives of preferential treatment is not sufficient for the effective management of coastal areas, such as ports. To improve management efficiency and fully integrate the coastal area with preferential treatment in the world economic relations, the authors consider it necessary to ensure commitment to the goals of sustainable development and propose a model for the implementation of the sustainable development principles, as exemplified by the free port of Vladivostok.
Candida auris Whole-Genome Sequence Benchmark Dataset for Phylogenomic Pipelines
Authors: Welsh Rory M. , Misas Elizabeth , Forsberg Kaitlin , Lyman Meghan , Chow Nancy A.
Published: March 2021

Candida auris is a multidrug-resistant pathogen that represents a serious public health threat due to its rapid global emergence, increasing incidence of healthcare-associated outbreaks, and high rates of antifungal resistance. Whole-genome sequencing and genomic surveillance have the potential to bolster C. auris surveillance networks moving forward. Laboratories conducting genomic surveillance need to be able to compare analyses from various national and international surveillance partners to ensure that results are mutually trusted and understood. Therefore, we established an empirical outbreak benchmark dataset consisting of 23 C. auris genomes to help validate comparisons of genomic analyses and facilitate communication among surveillance networks. Our outbreak benchmark dataset represents a polyclonal phylogeny with three subclades. The genomes in this dataset are from well-vetted studies that are supported by multiple lines of evidence, which demonstrate that the whole-genome sequencing data, phylogenetic tree, and epidemiological data are all in agreement. This C. auris benchmark set allows for standardized comparisons of phylogenomic pipelines, ultimately promoting effective C. auris collaborations.
A Novel Precision Approach to Overcome the “Addiction Pandemic” by Incorporating Genetic Addiction Risk Severity (GARS) and Dopamine Homeostasis Restoration
Authors: Blum Kenneth , Kazmi Shan , Modestino Edward J. , Downs Bill William , Bagchi Debasis , Baron David , McLaughlin Thomas , Green Richard , Jalali Rehan , Thanos Panayotis K. , Elman Igor , Badgaiyan Rajendra D. , Bowirrat Abdalla , Gold Mark S.
Published: March 2021

This article describes a unique therapeutic precision intervention, a formulation of enkephalinase inhibitors, enkephalin, and dopamine-releasing neuronutrients, to induce dopamine homeostasis for detoxification and treatment of individuals genetically predisposed to developing reward deficiency syndrome (RDS). The formulations are based on the results of the addiction risk severity (GARS) test. Based on both neurogenetic and epigenetic evidence, the test evaluates the presence of reward genes and risk alleles. Existing evidence demonstrates that the novel genetic risk testing system can successfully stratify the potential for developing opioid use disorder (OUD) related risks or before initiating opioid analgesic therapy and RDS risk for people in recovery. In the case of opioid use disorders, long-term maintenance agonist treatments like methadone and buprenorphine may create RDS, or RDS may have been in existence, but not recognized. The test will also assess the potential for benefit from medication-assisted treatment with dopamine augmentation. RDS methodology holds a strong promise for reducing the burden of addictive disorders for individuals, their families, and society as a whole by guiding the restoration of dopamine homeostasisthrough anti-reward allostatic neuroadaptations. WC 175.
Role of International Trade Competitive Advantage and Corporate Governance Quality in Predicting Equity Returns: Static and Conditional Model Proposals for an Emerging Market
Authors: Muzir Erol , Kizil Cevdet , Ceylan Burak
Published: March 2021

This paper aims to develop some static and conditional (dynamic) models to predict portfolio returns in the Borsa Istanbul (BIST) that are calibrated to combine the capital asset-pricing model (CAPM) and corporate governance quality. In our conditional model proposals, both the traditional CAPM (beta) coefficient and model constant are allowed to vary on a binary basis with any degradation or improvement in the country’s international trade competitiveness, and meanwhile a new variable is added to the models to represent the portfolio’s sensitivity to excess returns on the governance portfolio (BIST Governance) over the market. Some robust and Bayesian linear models have been derived using the monthly capital gains between December 2009 and December 2019 of four leading index portfolios. A crude measure is then introduced that we think can be used in assessing governance quality of portfolios. This is called governance quality score (GQS). Our robust regression findings suggest both superiority of conditional models assuming varying beta coefficients over static model proposals and significant impact of corporate governance quality on portfolio returns. The Bayesian model proposals, however, exhibited robust findings that favor the static model with fixed beta estimates and were lacking in supporting significance of corporate governance quality.
Measuring Disruptions in Last-Mile Delivery Operations
Authors: Muñoz-Villamizar Andrés , Solano-Charris Elyn L. , Reyes-Rubiano Lorena , Faulin Javier
Published: March 2021

The rapid growth of urbanisation and e-commerce has increased the number of home deliveries that need to be made in retail operations. Consequently, there is also an increase in unexpected incidents, such as adverse traffic, unavailability of parking space, and vehicle breakdowns. These disruptions result in delays, higher costs, and lower service levels in the last-mile delivery operation. Motivated by free, innovative, and efficient tools, such as the Google application programming interface (API) and Google OR, we built a model to measure the impact of disruptions in the last-mile delivery operation. Our model considers customers’ geographic information, speed estimation between nodes, routing optimisation, and disruption evaluation. Disruptions are considered here as external factors such as accidents and road works that imply the closure of or slow access to certain roads. Computational experiments, based on a set of real data from three different cities around the world, which contrast in size and characteristics (i.e., Boston, US; Bogotá, Colombia; and Pamplona, Spain), were conducted to validate our approach. The tests consider 50 different instances of up to 100 customers per city and analyse the impact of disruptions in terms of travelled time and distance. Our results provide managerial insights for key stakeholders (i.e., carriers, consumers, and government) to define policies and development plans that improve the resilience and capabilities of cities’ transportation systems.
Concrete Slab-Type Elements Strengthened with Cast-in-Place Carbon Textile Reinforced Concrete System
Authors: Kim Hyeong-Yeol , You Young-Jun , Ryu Gum-Sung , Ahn Gi-Hong , Koh Kyung-Taek
Published: March 2021

Although carbon textile reinforcement widely used to replace the steel reinforcing bars but the bonding strength of carbon textile is generally much smaller than that of common steel bars. This study examines the strengthening effect of concrete slab-type elements strengthened in flexure by carbon textile reinforcement according to the surface coating of textile and the amount of reinforcement. The effect of the surface coating of textile on the bond strength was evaluated through a direct pullout test with four different sizes of coating material. The surface coated specimens developed bond strength approximately twice that of the uncoated specimen. The flexural strengthening effect with respect to the amount of reinforcement was investigated by a series of flexural failure tests on full-scale reinforced concrete (RC) slab specimens strengthened by textile reinforced concrete (TRC) system. The flexural failure test results revealed that the TRC system-strengthened specimens develop load-carrying capacity that is improved to at least 150% compared to the non-strengthened specimen. The strengthening performance was not significantly influenced by the textile coating and was not proportional to the amount of reinforcement when this amount was increased, owing to the change in the failure mode. The outstanding constructability afforded by TRC strengthening was verified through field applications executing TRC strengthening by shotcreting on a concrete box culvert.
The Influence of Diet on Oxidative Stress and Inflammation Induced by Bacterial Biofilms in the Human Oral Cavity
Authors: Rowińska Ilona , Szyperska-Ślaska Adrianna , Zariczny Piotr , Pasławski Robert , Kramkowski Karol , Kowalczyk Paweł
Published: March 2021

The article is a concise compendium of knowledge on the etiology of pathogenic microorganisms of all complexes causing oral diseases. The influence of particular components of the diet and the role of oxidative stress in periodontal diseases were described. The study investigated the bacteriostatic effect of the diet of adults in in vivo and in vitro tests on the formation of bacterial biofilms living in the subgingival plaque, causing diseases called periodontitis. If left untreated, periodontitis can damage the gums and alveolar bones. Anaerobic bacteria, called periopathogens or periodontopathogens, play a key role in the etiopathogenesis of periodontitis. The most important periopathogens of the oral microbiota are bacteria of all complexes, including the red complex. The obtained results suggest the possibility of using a specific diet in the prevention and treatment of periodontal diseases-already treated as a disease of civilization. The quoted article is an innovative compilation of knowledge on this subject and it can be a valuable source of knowledge for professional hygienists, dentists, peridontologists, dentistry students and anyone who cares about proper oral hygiene. The obtained results suggest the possibility of using this type of diet in the prophylaxis of the oral cavity in order to avoid periodontitis.
Core Size and Interface Impact on the Exchange Bias of Cobalt/Cobalt Oxide Nanostructures
Authors: Ghoshani Maral , Mozaafari Morteza , Normile Peter S. , De Toro Jose A. , Al-Nabhani Abdulrahman
Published: March 2021

Two series of Co/Co-oxide nanostructures have been synthesized by the co-precipitation method followed by different reduction and oxidation processes in an attempt to optimize their exchange bias (EB) properties. The samples are characterized by X-ray diffraction, scanning and transmission electron microscopy, and SQUID (superconducting quantum interference device) magnetometry. The two series differ with respect to their average Co core grain sizes: in one (the l-series), the size is ≈100 nm, and in the other (the s-series, obtained using lower synthesis temperatures than the l-series), it is ≈10 nm. In the l-series, progressive oxidation yields an increase in the EB field together with a reduction in Co core size. In contrast, progressive oxidation in the s-series results in growth of the Co-oxide fraction at the expense of the Co core upon oxidation, which is accompanied by a decrease in the EB effect that is attributed to an ordering of the ferromagnetic–antiferromagnetic interface and therefore a reduction of uncompensated spins density. These results illustrate how the interface details become relevant only for small enough ferromagnetic cores.
Study of Structural, Magnetic, and Mossbauer Properties of Dy2Fe16Ga1−xNbx (0.0 ≤ x ≤ 1.0) Prepared via Arc Melting Process
Authors: Dahal Jiba N. , Ali Kalangala Sikkanther Syed , Mishra Sanjay R.
Published: March 2021

Intermetallic compounds of Dy2Fe16Ga1−xNbx (x = 0.0 to 1.00) were synthesized by arc melting. Samples were investigated for structural, magnetic, and hyperfine properties using X-ray diffraction, vibration sample magnetometer, and Mossbauer spectrometer, respectively. The Rietveld analysis of room temperature X-ray diffraction data shows that all the samples were crystallized in Th2Fe17 structure. The unit cell volume of alloys increased linearly with an increase in Nb content. The maximum Curie temperature Tc ~523 K for x = 0.6 sample is higher than Tc = 153 K of Dy2Fe17. The saturation magnetization decreased linearly with increasing Nb content from 61.57 emu/g for x = 0.0 to 42.46 emu/g for x = 1.0. The Mössbauer spectra and Rietveld analysis showed a small amount of DyFe3 and NbFe2 secondary phases at x = 1.0. The hyperfine field of Dy2Fe16Ga1−xNbx decreased while the isomer shift values increased with the Nb content. The observed increase in isomer shift may have resulted from the decrease in s electron density due to the unit cell volume expansion. The substantial increase in Tc of thus prepared intermetallic compounds is expected to have implications in magnets used for high-temperature applications.
An Econometric Approach Regarding the Impact of Fiscal Pressure on Equilibrium: Evidence from Electricity, Gas and Oil Companies Listed on the New York Stock Exchange
Authors: Batrancea Larissa
Published: March 2021

The matter of fiscal pressure is more current than ever in most countries around the world for various reasons. In the first place, disruptive phenomena such as financial crises put tremendous pressure on worldwide economies. Secondly, high taxes trigger an overall reduction in the level of investments aiming at creating stable and well-paid jobs. Thirdly, the income generated by the majority of taxpayers is subject to excessive taxation, which may fuel tax evasion acts. On these grounds, the article is the first empirical research investigating the impact of fiscal pressure on the financial equilibrium of energy companies listed on the New York Stock Exchange. The sample included 88 electricity, gas, and oil companies from around the world, which were analyzed over a time span of 16 years, including the periods before, during, and after the 2008 global financial crisis. The methodology entailed estimating econometric models via Panel Least Squares (cross-section weights) with and without time fixed effects. Empirical results showed that fiscal pressure had a stronger impact on the short-term and long-term equilibrium of electricity and oil companies than on the equilibrium of gas companies. The study can serve as a compass for the managers of energy companies interested in estimating the evolution of company equilibrium state when considering other potential financial downturns.
Combining Grammatical Evolution with Modal Interval Analysis: An Application to Solve Problems with Uncertainty
Authors: Contreras Ivan , Calm Remei , Sainz Miguel A. , Herrero Pau , Vehi Josep
Published: March 2021

Complex systems are usually affected by various sources of uncertainty, and it is essential to account for mechanisms that ensure the proper management of such disturbances. This paper introduces a novel approach to solve symbolic regression problems, which combines the potential of Grammatical Evolution to obtain solutions by describing the search space with context-free grammars, and the ability of Modal Interval Analysis (MIA) to handle quantified uncertainty. The presented methodology uses an MIA solver to evaluate the fitness function, which represents a novel method to manage uncertainty by means of interval-based prediction models. This paper first introduces the theory that establishes the basis of the proposed methodology, and follows with a description of the system architecture and implementation details. Then, we present an illustrative application example which consists of determining the outer and inner approximations of the mean velocity of the water current of a river stretch. Finally, the interpretation of the obtained results and the limitations of the proposed methodology are discussed.