bluuebunny/crossref_metadata_2025 huggingface dataset:
Differences in glycolytic metabolism between tissue-resident alveolar macrophages and recruited lung macrophages
Authors: Woods Parker S. , Mutlu Gökhan M.
Published: February 2025

Immunometabolism has emerged as a key area of focus in immunology and has the potential to lead to new treatments for immune-related diseases. It is well-established that glycolytic metabolism is essential for adaptation to hypoxia and for macrophage inflammatory function. Macrophages have been shown to upregulate their glycolytic metabolism in response to pathogens and pathogen-associated molecular patterns such as LPS. As a direct link to the external environment, the lungs’ distinctive nutrient composition and multiple macrophage subtypes provide a unique opportunity to study macrophage metabolism. This review aims to highlight how the steady-state airway and severely inflamed airway offer divergent environments for macrophage glycolytic metabolism. We describe the differences in glycolytic metabolism between tissue-resident alveolar macrophages, and other lung macrophages at steady-state and during inflammation/injury. We also provide an overview of experimental guidelines on how to assess metabolism at the cellular level using Seahorse-based bioenergetic analysis including a review of pharmacologic agents used to inhibit or activate glycolysis.
The dual role of neutrophils in sepsis-associated liver injury
Authors: Fang Lexin , Song Yu , Chen Jiangtao , Ding Yueping
Published: February 2025

Sepsis is often accompanied by liver injury and is associated with an increase in the number of circulating and hepatic neutrophils. In sepsis-associated liver injury, neutrophils exhibit phenotypic heterogeneity and perform both pro- and anti-inflammatory functions. Moreover, neutrophil dysfunction and neutrophil-associated immunosuppression are also involved in the pathogenesis of sepsis. Given the complex functionality of this cell type, the aim of this review was to describe the possible mechanistic role of neutrophils in sepsis-associated liver injury, with a brief introduction to neutrophil recruitment and subsequent discussion of the potential contributions of neutrophils to different subtypes of sepsis-associated liver injury.
Single-cell transcriptome sequencing reveals the cell populations and functions associated with stony coral Pocillopora damicornis
Authors: Diao Wenguang , Zhang Shengyao , Zhu Xueying , Wu Peiqi , Du Bingyan , Han Zhe , Liu Yunqing , He Chunpeng , Lu Zuhong
Published: February 2025

Introduction

Pocillopora damicornis, a key species of stony corals, has been the subject of considerable scientific study. However, the cellular composition of P. damicornis and the roles of these cells in endosymbiosis and biomineralization remain elusive. The development of single-cell technology has provided new opportunities for researching the cellular and molecular mechanisms underlying symbiosis and mineralization. Nevertheless, the stringent environmental requirements, the complexity of the cellular components, and the paucity of high-quality reference genomes of P. damicornis have posed significant challenges for single-cell transcriptome research.

Methods

In this study, we quantified the transcriptomic expression of P. damicornis by aligning its single-cell transcriptome (scRNA-seq) data to multiple species, including Stylophora pistillata, P. damicornis, and Pocillopora verrucosa. We determined the cell types of P. damicornis by comparing its cluster-specific genes with the published cell type-specific genes of S.pistillata and conducted gene function and enrichment analyses.

Results

Unsupervised clustering analysis yielded the identification of ten distinct cell populations, including epidermis cells, gastrodermis cells, algae-hosting cells, calicoblast, cnidocytes, and immune cells. In addition, we identified 53 genes that were highly similar to known sequences in the symbiotic zooxanthellae. These genes were mainly expressed in four different cell populations, corresponding to active symbiotic populations.

Conclusion

This study identified cell types closely associated with symbiosis and calcification in P. damicornis, along with their marker genes, which are consistent with the findings in S. pistillata. These results offer insights into the cellular functions and symbiotic mechanisms of P. damicornis.
Spatiotemporal composition and diversity of endophyte communities in Dracaena cambodiana on Hainan Island
Authors: Li Sipeng , Liu Yang , Yang Xin , Yang Yun , Peng Junxiang , Xu Yanhong , Wei Jianhe
Published: February 2025

Introduction

Dracaena cambodiana produces a red resin known as Dragon’s blood, which is used worldwide in traditional medicine and as a dye. The role of endophytes in the resin-formation process remains underexplored. Understanding the endophyte communities and their functional roles in resin production could enable the development of efficient induction techniques for resin production.

Methods

In this study, ITS and metagenomic sequencing analyzed endophyte communities’ characteristics and functional traits in different tissues and D. cambodiana across multiple wild populations on Hainan Island.

Results

We identified distinct fungal genera that were dominant in different tissues. Following injury, we observed significant changes in the expression of endophytic fungal genes. These changes indicated that metabolic pathways associated with resin metabolism, sucrose metabolism, signal transduction, and phenylalanine metabolism were likely involved in resin formation. Additionally, several glycosylation gene families were upregulated in the post-injury endophytic communities, which suggests a role in flavonoid transport and the reduction of autotoxic effects.

Discussion

Our results suggest that endophytes play a vital role in the resin-formation process of D. cambodiana. Isolating specific endophytes or using synthetic communities could potentially improve resin yields and avoid pathogenic fungi, ensuring safety. The findings from this study provide a theoretical basis for the development of high-efficiency resin induction techniques by targeting the dynamic changes in endophyte communities across tissues, regions, and resin formation stages.
Landscapes of the main components, metabolic and microbial signatures, and their correlations during stack “sweating” of Eucommiae Cortex
Authors: Wang Linfeng , Wu Mengxian , Gu Bingnan , Wang Erfeng , Wu Faliang , Yang Jiapeng , Guo Bing , Li Xingke , Zhang Pengpai
Published: February 2025

Introduction

“Sweating,” a key step in the processing and production of Eucommiae Cortex (EC), which plays a vital role in the formation of the medicinal quality of EC. However, the mechanism of the effect of this traditional treatment of herbs on the quality of herbs is still unclear.

Methods

In this study, high performance liquid chromatography (HPLC), UPLC/MS-based untargeted metabolomics and high-throughput sequencing were applied to investigate the dynamic changes of the main active ingredients, differential metabolites and bacterial communities in the process of “sweating” in EC. The samples were prepared by the traditional stacking “sweating” method, and the samples were collected once a day for five consecutive days.

Results

The results showed that the contents of the main active constituents, geniposidic acid (GPA), chlorogenic acid (CA), rutin (AU), pinoresinol diglucoside (PD) and total flavonoids (TFS), increased significantly after steaming, followed by a slight decrease. Furthermore, 807 metabolites were identified as crucial factors contributing to the metabolic alterations induced by the “sweating” process. Microbial diversity analysis showed considerable changes in microbiota characteristics, and the main functional microorganisms before and after “sweating” of EC were Gluconobacter, unclassified_c_Gammaproteobacteria, Pseudomonas, Pantoea, Pedobacter, and Parecoccus, which were involved in the five metabolic pathways of other secondary metabolites leading to significant changes in alkaloids, amino acid related compounds, flavonoids, phenylpropanoids and terpenoids.

Discussion

The correlation network established between core bacterial communities, active ingredients, and metabolic pathways elucidates the microbial regulation of EC quality during sweating. These findings provide a scientific foundation for optimizing processing duration and advancing quality control strategies through targeted microbial community management.
Physical activity and neuroplasticity in neurodegenerative disorders: a comprehensive review of exercise interventions, cognitive training, and AI applications
Authors: Ben Ezzdine Lamia , Dhahbi Wissem , Dergaa Ismail , Ceylan Halil İbrahim , Guelmami Noomen , Ben Saad Helmi , Chamari Karim , Stefanica Valentina , El Omri Abdelfatteh
Published: February 2025

This review aimed to elucidate the mechanisms through which (i) physical activity (PA) enhances neuroplasticity and cognitive function in neurodegenerative disorders, and (ii) identify specific PA interventions for improving cognitive rehabilitation programs. We conducted a literature search in PubMed, Medline, Scopus, Web of Science, and PsycINFO, covering publications from January 1990 to August 2024. The search strategy employed key terms related to neuroplasticity, physical exercise, cognitive function, neurodegenerative disorders, and personalized physical activity. Inclusion criteria included original research on the relationship between PA and neuroplasticity in neurodegenerative disorders, while exclusion criteria eliminated studies focusing solely on pharmacological interventions. The review identified multiple pathways through which PA may enhance neuroplasticity, including releasing neurotrophic factors, modulation of neuroinflammation, reduction of oxidative stress, and enhancement of synaptic connectivity and neurogenesis. Aerobic exercise was found to increase hippocampal volume by 1–2% and improve executive function scores by 5–10% in older adults. Resistance training enhanced cognitive control and memory performance by 12–18% in elderly individuals. Mind–body exercises, such as yoga and tai-chi, improved gray matter density in memory-related brain regions by 3–5% and enhanced emotional regulation scores by 15–20%. Dual-task training improved attention and processing speed by 8–14% in individuals with neurodegenerative disorders. We also discuss the potential role of AI-based exercise and AI cognitive training in preventing and rehabilitating neurodegenerative illnesses, highlighting innovative approaches to personalized interventions and improved patient outcomes. PA significantly enhances neuroplasticity and cognitive function in neurodegenerative disorders through various mechanisms. Aerobic exercise, resistance training, mind–body practices, and dual-task exercises each offer unique cognitive benefits. Implementing these activities in clinical settings can improve patient outcomes. Future research should focus on creating personalized interventions tailored to specific conditions, incorporating personalized physical exercise programs to optimize cognitive rehabilitation.
The assessment of autonomic nervous function in patients with gastrointestinal malignancies and its relationship with clinical characteristics
Authors: Xiwen Zhang , Qiyun Feng , Chuqiao Li , Anqi Jiang , Zhenzhen Wu , Qiong Deng , Yu Peng , Chunlin Wang
Published: February 2025

Introduction

The gastrointestinal tract is the organ most extensively distributed by autonomic nerves, and researches have indicated a relationship between automatic nerves and the progression of gastrointestinal cancers. This study aimed to evaluate the autonomic nervous function in patients with gastrointestinal cancer and to explore its relationship with clinical characteristics.

Methods

We employed the Composite Autonomic Symptom Score 31 (COMPASS-31) questionnaire and cardiovascular autonomic reflex tests (CARTs) to evaluate autonomic nervous function, while also conducting a thorough analysis of clinical data.

Results

Our results showed that low white blood cell (WBC) count (OR = 0.461, 95% CI: 0.218–0.976, p = 0.043) and increased maximum tumor diameter (OR = 1.619, 95% CI: 1.025–2.555, p = 0.039) were risk factors for autonomic dysfunction according to the COMPASS-31 assessment. While hypertension (OR = 5.747, 95% CI: 1.186–27.862, p = 0.030) and elevated platelet-to-albumin ratio (PAR) (OR = 1.256, 95% CI: 1.025–1.540, p = 0.028) were identified as independent risk factors for autonomic dysfunction based on the CARTs results. Combining the findings from COMPASS-31 and CARTs revealed that older age (OR = 1.133, 95% CI: 1.015–1.264, p = 0.027) and vascular invasion (OR = 7.706, 95% CI: 1.391–42.684, p = 0.019) were also independent risk factors for autonomic dysfunction.

Conclusion

Our findings reveal that these specific factors related to gastrointestinal cancers significantly influence autonomic nervous function. It is essential to evaluate autonomic nervous function and its associated risk factors in patients with gastrointestinal malignancies, which provide new insights into the intervention strategies for cancer diseases.
Association of probiotic supplementation and cardiovascular risk profiles of patients with coronary artery disease—a cross-sectional analysis of the NHANES database between 1999–2019
Authors: Palathinkara Murali , Aljadah Michael , Thorgerson Abigail , Dawson Aprill Z. , Widlansky Michael E.
Published: February 2025

Background

Atherosclerotic cardiovascular disease (ASCVD) is a chronic inflammatory disease that leads to adverse events such as myocardial infarctions and stroke. Gut microbiome modulation is a promising target to reduce chronic inflammation and improve outcomes for patients with coronary artery disease (CAD). Risk profile patterns of CAD patients who target gut health with probiotics could provide insight into how gut modulation improves CAD clinical biomarkers. This study aims to evaluate the association between probiotic use and clinical markers of known atherosclerotic risk factors, in patients with CAD.

Methods

We conducted a cross-sectional large-database study using National Health and Nutrition Examination Survey (NHANES) data from years 1999–2020. The cohort included adults with at least a diagnosis of coronary heart disease, angina, and heart attack or two of the following: diabetes, high blood pressure, and high cholesterol. Analyses of clinical biomarkers compared probiotic to non-probiotic groups, between probiotic type groups, and between probiotic supplement strains.

Results

Our cohort included 14,992 survey responders. After weighting, this sample represented 46,217,980 US adults. There were 4,062,022 adults exposed to probiotics, 763,288 to probiotic supplements and 3,179,008 to probiotic foods. Probiotic exposure was associated with lower A1c (p < 0.001), lower triglycerides (p < 0.001), lower ASCVD risk score (p = 0.01) and higher HDL-C (p < 0.001). Probiotic supplement exposure was associated with lower LDL-C (p = 0.003) and total cholesterol (p = 0.047).

Conclusion

Our study reinforces the beneficial association between probiotic ingestion and cardiovascular health in patients with existing atherosclerotic disease. Further studies to better determine potential mechanistic connections between the gut microbiota on cardiovascular risk factors is warranted.
Development and validation of a prognostic nomogram for patients with malignant peritoneal mesothelioma
Authors: Wang Xiaohan , Liu RuiTing , Wang Chunli , Sun Jingjing , Yang Dongliang
Published: February 2025

Background

Malignant peritoneal mesothelioma(MPM) is a highly aggressive malignant tumor that originates from peritoneal mesothelial cells. Due to the rarity of MPM, there are few survival prediction models specifically for visualization of malignant peritoneal mesothelioma.

Objective

This study aimed to develop a nomogram for the overall survival of MPM based on the Surveillance, Epidemiology, and End Results (SEER) database and the data of Cangzhou People’s Hospital were used for external verification.

Methods

Patients screened from the SEER database were divided into a training group and an internal verification group in a 7:3 ratio, with data from Cangzhou People’s Hospital used as the external verification group. Cox proportional hazard regression was utilized to identify significant factors, and nomograms for 6-month, 12-month, and 18-month overall survival were developed. The performance of the nomogram was assessed using consistency index, calibration curve, and K-M curve.

Results

Age, sex, histology, surgery, tumor size, chemotherapy, differentiated and the number of organ metastases were significant risk factors (p<0.05) and were included in the nomogram.The area under the subject worker curve at 6,12,18 months overall survival (AUC) was 0.782,0.784,0.766 for the training group, 0.804,0.791,0.796 for the internal verification group, 0.767,0.749,0.783 for the external verification group. The predicted correction curve was in good agreement with the observed results. The Kaplan-Meier curves of different risk groups showed significant differences.

Conclusion

This study represents the first visual prognostic model of MPM and the initial incorporation of organ metastasis into MPM prognostic factors. The nomograph serves as a reliable tool for clinicians to personalize overall survival prediction and maximize patient benefits by identifying the most effective treatment.
Targeted therapy in pediatric central nervous system tumors: a review from the National Pediatric Cancer Foundation
Authors: Siegel Benjamin I. , Patil Prabhumallikarjun , Prakash Akul , Klawinski Darren M. , Hwang Eugene I.
Published: February 2025

Central nervous system tumors represent the leading cause of cancer-related mortality in children. Conventional therapies of surgery, radiation, and cytotoxic chemotherapy have insufficient efficacy for some pediatric CNS tumors and are associated with significant morbidity, prompting an ongoing need for novel treatment approaches. Identification of molecular alterations driving tumorigenesis has led to a rising interest in developing targeted therapies for these tumors. The present narrative review focuses on recent progress in targeted therapies for pediatric CNS tumors. We outline the key implicated cellular pathways, discuss candidate molecular therapies for targeting each pathway, and present an overview of the clinical trial landscape for targeted therapies in pediatric CNS tumors. We then discuss challenges and future directions for targeted therapy, including combinatorial approaches and real-time drug screening for personalized treatment planning.
SMARCA4-deficient undifferentiated carcinoma co-existing with primary endometrial gastric (or gastrointestinal) -type carcinoma: a case report
Authors: Yang Fan , Fu Haixiao , Zheng Xiang , Yang Yuzhong , Zhou Quyan , Liang Yuanna , Zheng Jinhua , Lin Jing
Published: February 2025

Dedifferentiated endometrial carcinoma (DEC) is a rare and highly malignant endometrial tumor consisting of both undifferentiated endometrial carcinoma (UEC) and differentiated components (that are typically grade 1 or 2 endometrioid carcinomas). By contrast, the coexistence of UEC with a high-grade endometrial carcinoma has not been well reported. Primary endometrial gastric (gastrointestinal)-type carcinoma (PEGT-carcinoma) is a newly classified and rare type of female genital tract cancer, this neoplasm belongs to high-grade endometrial carcinoma and tends to have a poor outcome. In this study, we reported an atypical case of DEC composed of SMARCA4-deficient UEC and PEGT-carcinoma and reviewed its clinicopathological features.
Adoptive cell therapy against tumor immune evasion: mechanisms, innovations, and future directions
Authors: Ruan Liqin , Wang Lu
Published: February 2025

Tumors employ a range of strategies to evade detection and eradication by the host’s immune system. These include downregulating antigen expression, altering antigen presentation processes, and inhibiting immune checkpoint pathways. etc. Adoptive Cell Therapy (ACT) represents a strategy that boosts anti-tumor immunity. This is achieved by amplifying or genetically engineering immune cells, which are either sourced from the patient or a donor, in a laboratory setting. Subsequently, these cells are reintroduced into the patient to bolster their immune response against cancer. ACT has successfully restored anti-tumor immune responses by amplifying the activity of T cells from patients or donors. This review focuses on the mechanisms underlying tumor escape, including alterations in tumor cell antigens, the immunosuppressive tumor microenvironment (TME), and modulation of immune checkpoint pathways. It further explores how ACT can avddress these factors to enhance therapeutic efficacy. Additionally, the review discusses the application of gene-editing technologies (such as CRISPR) in ACT, highlighting their potential to strengthen the anti-tumor capabilities of T cells. Looking forward, the personalized design of ACT, combined with immune checkpoint inhibitors and targeted therapies, is expected to significantly improve treatment outcomes, positioning this approach as a key strategy in the field of cancer immunotherapy.
Development and validation of a prediction model for VTE risk in gastric and esophageal cancer patients
Authors: Zheng Xingyue , Wu Liuyun , Li Lian , Wang Yin , Yin Qinan , Han Lizhu , Wu Xingwei , Bian Yuan
Published: February 2025

Objective

This study focuses on the risk of venous thromboembolism (VTE) in patients with gastric or esophageal cancer (GC/EC), investigating the risk factors for VTE in this population. Utilizing machine learning techniques, the research aims to develop an interpretable VTE risk prediction model. The goal is to identify patients with gastric or esophageal cancer who are at high risk of VTE at an early stage in clinical practice, thereby enabling precise anticoagulant prophylaxis and thrombus management.

Methods

This study is a real-world investigation aimed at predicting VTE in patients with GC/EC. Data were collected from inpatients diagnosed with GC/EC at Sichuan Provincial People’s Hospital between 1 January 2018, and 31 June 2023. Using nine supervised learning algorithms, 576 prediction models were developed based on 56 available variables. Subsequently, a simplified modeling approach was employed using the top 12 feature variables from the best-performing model. The primary metric for assessing the predictive performance of the models was the area under the ROC curve (AUC). Additionally, the training data used to construct the best model in this study were employed to externally validate several existing assessment models, including the Padua, Caprini, Khorana, and COMPASS-CAT scores.

Results

A total of 3,742 cases of GC/EC patients were collected after excluding duplicate visit information. The study included 861 (23.0%) patients, of which 124 (14.4%) developed VTE. The top five models based on AUC for full-variable modeling are as follows: GBoost (0.9646), Logic Regression (0.9443), AdaBoost (0.9382), CatBoost (0.9354), XGBoost (0.8097). For simplified modeling, the models are: Simp-CatBoost (0.8811), Simp-GBoost (0.8771), Simp-Random Forest (0.8736), Simp-AdaBoost (0.8263), Simp-Logistic Regression (0.8090). After evaluating predictive performance and practicality, the Simp-GBoost model was determined as the best model for this study. External validation of the Padua score, Caprini score, Khorana score, and COMPASS-CAT score based on the training set of the Simp-GBoost model yielded AUCs of 0.4367, 0.2900, 0.5000, and 0.3633, respectively.

Conclusion

In this study, we analyzed the risk factors of VTE in GC/EC patients, and constructed a well-performing VTE risk prediction model capable of accurately identifying the extent of VTE risk in patients. Four VTE prediction scoring systems were introduced to externally validate the dataset of this study. The results demonstrated that the VTE risk prediction model established in this study held greater clinical utility for patients with GC/EC. The Simp-GB model can provide intelligent assistance in the early clinical assessment of VTE risk in these patients.
Repurposing hydrochlorothiazide (HCTZ) for colorectal cancer: a retrospective and single center study
Authors: Foo Dominic Chi-Chung , Li Jiaxi , Huang Zheng , Sui Siming , Sin Ryan Wai-Yan , Man Abraham Tak Ka , Law Wai-Lun , Ng Lui
Published: February 2025

Background

Anti-hypertensive drugs have been reported to demonstrate anti-inflammatory and anti-angiogenic effects. This study aims to investigate the association between anti-hypertensive drugs and the prognosis of colorectal cancer (CRC) patients.

Methods

Clinical data of 1134 CRC patients with hypertensions and the prescription of anti-hypertensive drugs who had undergone curative surgery in our hospital between 2005 and 2015 were retrieved. Their survival data and immune cell population in circulatory blood were compared among different types of anti-hypertensive drugs and overall CRC patients.

Results

The 5-year overall survival for the antihypertensives-treated patients (65.2%) was higher than the CRC patients in Hong Kong (58.2%). Hydrochlorothiazide (HCTZ) group showed the best prognosis (79.1%) among different antihypertensive drug, particularly for advance stage or elderly patients, which are poor prognostic factors for overall CRC patients, demonstrated an obviously improved prognosis upon HCTZ treatment. Moreover, our data showed the recurrence rate was significantly lower for HCTZ group (18.3%) compared to non-HCTZ group (26.8%) and the reported rate (31%) of CRC patients in Hong Kong. Finally, patients with a lower pre-operative basophil level showed better overall and disease-free survival following HCTZ treatment.

Conclusion

This study demonstrated the association of HCTZ treatment with a better prognosis of CRC patients.
Identification of glycolysis-related gene signatures for prognosis and therapeutic targeting in idiopathic pulmonary fibrosis
Authors: Gao Han , Sun Zhongyi , Hu Xingxing , Song Weiwei , Liu Yuan , Zou Menglin , Zhu Minghui , Cheng Zhenshun
Published: February 2025

Background

Glycolysis plays a crucial role in fibrosis, but the specific genes involved in glycolysis in idiopathic pulmonary fibrosis (IPF) are not well understood.

Methods

Three IPF gene expression datasets were obtained from the Gene Expression Omnibus (GEO), while glycolysis-related genes were retrieved from the Molecular Signatures Database (MsigDB). Differentially expressed glycolysis-related genes (DEGRGs) were identified using the “limma” R package. Diagnostic glycolysis-related genes (GRGs) were selected through least absolute shrinkage and selection operator (LASSO) regression regression and support vector machine-recursive feature elimination (SVM-RFE). A prognostic signature was developed using LASSO regression, and time-dependent receiver operating characteristic (ROC) curves were generated to evaluate predictive performance. Single-cell RNA sequencing (scRNA-seq) data were analyzed to examine GRG expression across various cell types. Immune infiltration analysis, Gene Set Enrichment Analysis (GSEA), and Gene Set Variation Analysis (GSVA) were performed to elucidate potential molecular mechanisms. A bleomycin (BLM)-induced pulmonary fibrosis mouse model was used for experimental validation via reverse transcription-quantitative polymerase chain reaction (RT-qPCR).

Results

14 GRGs (VCAN, MERTK, FBP2, TPBG, SDC1, AURKA, ARTN, PGP, PLOD2, PKLR, PFKM, DEPDC1, AGRN, CXCR4) were identified as diagnostic markers for IPF, with seven (ARTN, AURKA, DEPDC1, FBP2, MERTK, PFKM, SDC1) forming a prognostic model demonstrating predictive power (AUC: 0.831–0.793). scRNA-seq revealed cell-type-specific GRG expression, particularly in macrophages and fibroblasts. Immune infiltration analysis linked GRGs to imbalanced immune responses. Experimental validation in a bleomycin-induced fibrosis model confirmed the upregulation of GRGs (such as AURKA, CXCR4). Drug prediction identified inhibitors (such as Tozasertib for AURKA, Plerixafor for CXCR4) as potential therapeutic agents.

Conclusion

This study identifies GRGs as potential prognostic biomarkers for IPF and highlights their role in modulating immune responses within the fibrotic lung microenvironment. Notably, AURKA, MERTK, and CXCR4 were associated with pathways linked to fibrosis progression and represent potential therapeutic targets. Our findings provide insights into metabolic reprogramming in IPF and suggest that targeting glycolysis-related pathways may offer novel pharmacological strategies for antifibrotic therapy.
Decoding interaction between mitochondria and endoplasmic reticulum in ischemic myocardial injury: targeting natural medicines
Authors: Zhang Chuxin , Chang Xing , Zhao Dandan , He Yu , Dong Guangtong , Gao Lin
Published: February 2025

Ischemic cardiomyopathy (ICM) is a special type or end stage of coronary heart disease or other irreversible ischemic myocardial injury. Inflammatory damage to coronary vessels is a crucial factor in causing stenosis or occlusion of coronary arteries, resulting in myocardial ischemia and hypoxia, but it is also an aspect of cardioprotection that is often overlooked. This review discusses the mechanisms of vascular injury during ICM, in which inflammation and oxidative stress interact and trigger cell death as the cause of coronary microvascular injury. Imbalances in endoplasmic reticulum function and mitochondrial quality control are important potential drivers of inflammation and oxidative stress. In addition, many studies have confirmed the therapeutic effects of Chinese herbal medicines and their natural monomeric components on vascular injuries. Their mitochondrial quality control and endoplasmic reticulum protection mechanisms as well as their role in combating improvements in vascular endothelial function and attenuating vascular injury are also summarized, with a perspective to provide a reference for pathologic understanding, drug research, and clinical application of ICM-associated coronary microvascular injury.
Research progress on ferroptosis in Myelodysplastic syndromes
Authors: Yang Yifan , Han Jiongping , Wei Yuxin , Jin Jiacheng , Feng Weiyin
Published: February 2025

Myelodysplastic syndromes (MDS) are a group of malignancies characterized by clonal proliferation of hematopoietic stem cells, ineffective hematopoiesis, peripheral cytopenias, and a high risk of transformation to acute myeloid leukemia. Current therapeutic strategies for MDS have limited efficacy. Thus, identifying new therapeutic targets and prognostic biomarkers is a critical future research direction. Ferroptosis, a new type of iron-dependent programmed cell death, has become a recent hotspot in the field of oncology research. Recent results have demonstrated that iron metabolism, lipid metabolism, and other pathways can be targeted to induce ferroptosis in MDS cells. In addition, ferroptosis-related genes are of significance in the prognosis and diagnosis of MDS. This article reviews the current research progress on ferroptosis in MDS, including its potential for targeting as a therapeutic intervention strategy.
Pollen image manipulation and projection using latent space
Authors: Mills Ben , Zervas Michalis N. , Grant-Jacob James A.
Published: February 2025

Understanding the structure of pollen grains is crucial for the identification of plant taxa and the understanding of plant evolution. We employ a deep learning technique known as style transfer to investigate the manipulation of microscope images of these pollens to change the size and shape of pollen grain images. This methodology unveils the potential to identify distinctive structural features of pollen grains and decipher correlations, whilst the ability to generate images of pollen can enhance our capacity to analyse a larger variety of pollen types, thereby broadening our understanding of plant ecology. This could potentially lead to advancements in fields such as agriculture, botany, and climate science.
Rare earth elements affect the growth and fitness of free-floating plant Lemna minor L.
Authors: Gjata Isidora , Tommasi Franca , De Leonardis Silvana , Paciolla Costantino
Published: February 2025

Rare earth elements (REEs) are increasingly utilized in modern technologies but are now recognized as emerging pollutants, with limited understanding of their impact on aquatic ecosystems. In this study, the effects of selected REEs (Ce, Nd, Gd, Dy, Yb, Ho, and Lu) in chloride form were evaluated on Lemna minor L., a plant species widely used as tool for the biomonitoring of the aquatic environments. Under controlled laboratory conditions, growth parameters, pigment content, oxidative stress markers, total antioxidant capacity, and antioxidant enzyme activities were assessed at millimolar concentrations over different exposure periods. L. minor exhibited tolerance to low millimolar concentrations of REEs over short-term exposure. However, prolonged exposure to high concentrations resulted in toxicity, characterized by growth inhibition, chlorophyll degradation, increased lipid peroxidation, and oxidative stress. Particularly, a hormetic response was observed for cerium, with stimulation at low concentrations and inhibition at higher levels, while dysprosium did not significantly affect growth. Other tested REEs induced varying degrees of stress, with holmium and lutetium causing the most severe toxic effects. Changes in antioxidant enzyme activities indicated a differential activation of stress responses depending on the REE type. These findings highlight the necessity for continuous monitoring of REEs in aquatic systems and support the use of L. minor as a valuable tool for environmental risk assessment.
Light-mediated activation of PpPSY enhances β-carotene accumulation in pear fruit peel
Authors: Zhang Li , Du Wei , Tu Junfan , Zhu Hongyan , Li Xianming
Published: February 2025

Light is a key environmental factor that regulates fruit development and influences several important quality traits, including pericarp color. In pear fruits, carotenoids are the primary determinant of pericarp color. However, the molecular mechanisms underlying light-mediated carotenoid accumulation remain poorly understood. This study investigated the carotenoid contents in the peels of non-bagged (light-exposed) and bagged (shaded) pear fruits (Cuiguan, Pyrus pyrifolia) and revealed a significant differences in β-carotene content between the two treatments. Transcriptome analysis revealed that the expression of phytoene synthase (PSY) was downregulated in bagged fruits, highlighting the regulatory role of PSY in carotenoid metabolism. To further validate this, we transiently overexpressed PSY, which resulted in a marked increase in β-carotene levels at the injection site. Conversely, transient silencing of PSY led to a significant reduction in the β-carotene content, confirming the pivotal role of PSY in regulating β-carotene accumulation. Promoter analysis revealed that agamous-like 8 (AGL8) directly binds to the PSY promoter to activate its transcription. Protein−protein interaction assays demonstrated that AGL8 interacts with LEAFY (LFY), thereby increasing PSY expression. In conclusion, the AGL8-LFY complex coactivates PSY expression, regulating β-carotene accumulation in pear fruit. This study provides new insights into the regulatory network governing fruit peel coloration, with potential applications for cultivation strategies to improve fruit quality.
Exploring the feasibility of collecting music and wellbeing data to examine intentional listening using a mobile-ESM application
Authors: McFerran Katrina Skewes , Krause Amanda E. , Osborne Margaret S.
Published: February 2025

This brief report describes a small-scale feasibility study investigating the use of mobile Experience Sampling Methodology (ESM) for collecting data on intentionality in music listening for well-being. Sixteen university students used the MuPsych app (Randall and Rickard, 2012) for a 2-week pilot study (resulting in 263 music listening episode responses), with seven participating in semi-structured follow-up interviews. Data was collected at baseline and then triggered by mobile music listening episodes at 0, 5 and 20 min. Baseline measures were of wellbeing; and listening episode data included music choice, purpose, context, and mood. After assigning listeners to languishing, moderate, or flourishing wellbeing categories, differences became apparent in participants’ experiences of listening to music. Several challenges to feasibility were experienced in self-selection and biased reporting by participants as well as technological limitations of data collection techniques. Recommendations for future ESM studies of everyday music listening are offered.
The daily relations between workplace anger, coping strategies, work outcomes, and workplace affiliation
Authors: Umbra Robin , Fasbender Ulrike
Published: February 2025

This study examines the daily relations among workplace anger, coping strategies, work outcomes, and employee dispositions using a conceptual framework based on affective events theory and cognitive perspectives on emotions. A sample of 214 full-time employees took part in a two-week study, contributing 1,611 daily observations through an experience sampling approach. Contrary to the assumption that workplace anger always detrimentally relates to work outcomes, the results showed a nonsignificant relation between workplace anger and workplace resource depletion, as well as a positive link between workplace anger and goal achievement. These relations were dependent on the coping strategies used by employees in response to anger-inducing situations, as well as their attitudes toward workplace affiliation. These findings suggest the need to expand affective events theory to include coping strategies as a mediator between affective responses and work outcomes. They also highlight the importance of integrating employee-level factors into organizational research models.
Vulnerabilities in migrant live-in care arrangements for people with dementia: a comparative analysis of experts’ insights from Germany and Israel
Authors: Ulitsa Natalie , Nebowsky Anna Eva , Ayalon Liat , Schweda Mark , von Kutzleben Milena
Published: February 2025

Background

The global rise in dementia among older adults has led to an increased reliance on migrant live-in caregivers, particularly in countries like Germany and Israel. This triadic care arrangement, involving persons with dementia, their families, and migrant live-in caregivers, presents unique challenges and vulnerabilities. These vulnerabilities, deeply intertwined with ethical concerns, are shaped by the socio-cultural and legal contexts of each country. This study aims to explore these vulnerabilities through a comparative analysis of expert experiences in Germany and Israel.

Method

A qualitative study was conducted using semi-structured interviews with 24 experts—14 from Israel and 10 from Germany—who have extensive experience in dementia care or migrant caregiving. The interviews were analyzed through qualitative content analysis, focusing on six dimensions of vulnerability: physical, psychological, relational/interpersonal, moral, socio-cultural-political-economic, and existential/spiritual.

Results

The analysis revealed that all parties in the care triad—persons with dementia, migrant live-in caregivers, and family members—experience distinct yet interconnected vulnerabilities. These vulnerabilities are deeply entangled, manifesting in complex, interrelated ways both within each party and between the different parties in this triadic arrangement. The study also highlighted both similarities and differences in expert experiences between Germany and Israel, reflecting the unique socio-cultural and legal contexts of each country.

Conclusions

The study underscores the multifaceted and interdependent nature of vulnerabilities in migrant live-in care arrangements for people with dementia. By comparing expert insights from Israel and Germany, the research highlights the critical role of national policies and cultural contexts in shaping these vulnerabilities, leading to distinct experiences and challenges in each country. Addressing these vulnerabilities is essential for improving the quality of care and the well-being of all parties involved in the triadic care arrangement.
Healing hearts: mind-body therapy for mothers after stillbirth’s silent grief
Authors: Bar Vered , Hermesh Tamar , Reshef Piki , Hermetz Shoshy , Hertz-Palmor Nimrod , Gothelf Doron , Mosheva Mariela
Published: February 2025

Introduction

Approximately 0.75% of pregnancies end with stillbirth, often leading to depression, anxiety, posttraumatic stress symptoms and suicidality. Knowledge regarding effective treatment options is lacking. In this retrospective cohort study we present a mind-body group therapy treatment protocol that was adapted for women and their partners who suffered stillbirth and report on its clinical effectiveness. Additionally, we identified demographic and clinical factors that were associated with clinical response.

Methods

Sixty-one women who coped with stillbirth were enrolled to a mind-body group therapy. Questionnaires assessing symptoms of depression, anxiety, and post-traumatic stress were administered to the women before and after the group intervention.

Results

As expected, we found high rates of depression, state and trait anxiety and post-traumatic symptoms in our cohort before mind-body group therapy. At completion of treatment, the symptoms of depression, state anxiety, post-traumatic stress and suicidality significantly decreased. Improvement in symptoms of depression and post-trauma at follow-up was positively associated with severity of symptoms at baseline and with antidepressants treatment, and negatively associated with the number of children. Time since stillbirth was positively associated with the degree of improvement in posttraumatic symptoms only.

Conclusions

Our findings suggest that mind-body group therapy may be associated with improvements in depression, post-traumatic stress symptoms, state anxiety, and suicidal ideation in women following stillbirth. Further research, including a control group is crucial for understanding of effective tools to treat this at-risk population.
Burden, trends, and predictions of low physical activity-related diseases in China: analysis from the Global Burden of Disease Study, 1990–2021, with projections to 2035
Authors: Zhang Chuan , Chen Quanzheng , Yin Fuqiang , Qin Lanyan , Zhang Shuna
Published: February 2025

Objective

To evaluate the burden of disease related to low physical activity in China, examine its trends over time, and predict future trends up to 2035 to inform preventive actions.

Methods

Using Global Burden of Disease Study 2021 (GBD2021) data, we analyzed the burden of disease associated with low physical activity, employing Deaths and Disability-Adjusted Life Years (DALYs) as indicators. Trends from 1990 to 2021 were examined using the Estimated Annual Percentage Change (EAPC) method, and future projections were made using the BAPC model.

Results

The number of deaths attributable to low physical activity rose from 48,882 in 1990 to 148,152 in 2021, with the age-standardized death rate decreasing slightly from 8.39 to 8.18 per 100,000 (EAPC = 0.07). DALYs increased from 1,246,888 to 3,254,644, while the age-standardized DALYs rate fell from 168.93 to 162.52 per 100,000 (EAPC = −0.08). The DALY rate for diabetes increased from 30.12 to 36.54 per 100,000 (EAPC = 0.42); ischemic heart disease death rate rose from 2.86 to 3.52 per 100,000 (EAPC = 1.12), and the DALY rate increased from 40.72 to 46.05 per 100,000 (EAPC = 0.8). The highest death and DALY rates were observed in individuals over 95 years old. Females had higher death and DALY rates than males, with a death rate of 8.29 per 100,000 and a DALY rate of 197.98 in 2021. The BAPC model predicts that by 2035, deaths will reach 161,280 and DALYs will reach 3,523,135.

Conclusion

From 1990 to 2021, the age-standardized death and DALY rates associated with low physical activity in China declined, yet the absolute number of deaths and DALYs increased due to population growth and aging. Special attention is needed for diabetes and ischemic heart disease, as their burdens have significantly risen. Although females are more affected by low physical activity than males, the increase in the burden among males has been more pronounced in recent years. The impact of low physical activity intensifies with age, particularly among the older adult. Thus, targeted strategies are essential to mitigate the burden of low physical activity in China.
Time trends in stomach cancer mortality across the BRICS: an age-period-cohort analysis for the GBD 2021
Authors: Liu Dan , Liu Hao , Wu Yuhang , Wang Weihong
Published: February 2025

Objectives

Stomach cancer is one of the leading causes of cancer death, and its epidemiologic characteristics are regionally heterogeneous worldwide. The BRICS nations (Brazil, Russian Federation, India, China, and South Africa) have markedly increasing influences on the international stage. We aim to investigate time trends in stomach cancer mortality among the BRICS countries from 1982 to 2021.

Methods

Data for this study were obtained from the Global Burden of Disease (GBD) 2021 public dataset to investigate the deaths, all-age mortality rate, and age-standardized mortality rate (ASMR) of stomach cancer. The age-period-cohort (APC) model was employed to estimate net drift, local drift, age-specific curves, and period (cohort) relative risks, and the Bayesian generalized linear model was employed to evaluate the relationship between food intake and mortality rate.

Results

In 2021, there were approximately 572,000 stomach cancer deaths across the BRICS, accounting for 59.9% of global death. Russian Federation exhibited the most significant reduction in ASMR of stomach cancer among the BRICS. In contrast, China continued to report the highest number of stomach cancer deaths. The risk of mortality associated with stomach cancer exhibited a marked increase with advancing age, both within these countries and at the global level. PUFA, sodium, calcium and trans fat may have an impact on the mortality rate of stomach cancer. Favorable trends in period and birth cohort effects were observed in these five nations over the past decades.

Conclusion

BRICS countries have made varying progress in reducing stomach cancer mortality. Given the diverse environments, it is recommended to progressively develop customized stomach cancer prevention strategies, utilizing available resources. Healthcare services should be extended to all age groups, with a particular emphasis on vulnerable populations.
Social differences between the general population and tuberculosis patients in Slovakia
Authors: Vyšehradský Peter , Solovič Ivan , Kotúľová Lucia , Grendár Marián , Rákošová Monika , Hudečková Henrieta , Vyšehradský Robert
Published: February 2025

Objectives

Point out the social specifics of patients with tuberculosis, which persist despite the socioeconomic development of Slovak society.

Methods

A questionnaire survey was conducted on a sample of consecutive patients with newly diagnosed tuberculosis during the first half of 2023. The collected data were aggregated and compared with the results of European Health Interview Survey (EHIS) in 2019.

Results

We found significant differences in the distribution of the level of education, labour status, nutritional and marital status, financial poverty, smoking habits, alcohol consumption and number of rooms in the dwelling between the general population and TB patients in Slovakia. Moreover, a significant trend in the proportions was observed across contingency table categories for all ordinal variables with more than two levels.

Conclusion

There are several significant social differences between patients with tuberculosis and the general Slovak population.