bluuebunny/crossref_metadata_2025 huggingface dataset:
Corn Silage as a Total Diet with by-Products of the Babassu Agroindustry in the Feed of Confined Ruminants
Authors: Sá Cledson , Zanine Anderson , Ferreira Daniele , Parente Henrique , Parente Michelle , Santos Edson Mauro , Lima Anny Graycy , Santos Francisco Naysson , Pereira Danillo , Sousa Francisca Claudia de , Costa Renata , Castro Carlos Rodolfo , Alves Guilherme Ribeiro , Dórea João Ricardo
Published: February 2023

This study aimed to evaluate the chemical composition of total ration silage (TRS) containing two babassu by-products to replace the corn. The silages were formulated to meet the requirements of sheep for an average daily gain of 200 g/day. A completely randomized experimental design was used with four treatments and five replications. The treatments consisted of CS: Corn silage (Control); TRSS: Corn silage with corn and soybean meal; TRSF: Total ration silage with babassu mesocarp flour; and TRSC: Total feed silage with babassu cake. The chemical composition of the silages had a significant difference (p < 0.05) for all variates. There was a significant difference (p < 0.001) for gas (LG, p < 0.001) and effluent losses (LE, p < 0.001), dry matter recovery (DMR, p < 0.001), buffer capacity (BC, p < 0.001), lactic acid (LA, p < 0.001), butyric acid (BA, p < 0.001), lactic acid/fermentation products (LA:FP, p < 0.001), and ammoniacal nitrogen (NH3-N, p < 0.001). The babassu by-products can replace ground corn by up to 50% in total ration silage and improve the fermentation profile and nutritional value of the silage, meeting the nutritional requirements of finishing sheep.
Microwaves Induce Histological Alteration of Ovaries and Testis in Rhynchophorus ferrugineus Oliv. (Coleoptera: Curculionidae)
Authors: Martano Manuela , Massa Rita , Restucci Brunella , Caprio Emilio , Griffo Raffaele , Power Karen , Maiolino Paola
Published: February 2023

The Red Palm Weevil (RPW) is one of the major pests of palms, frequently leading to the plants death. Action plans and the development of bio/physical strategies to contrast RPW diffusions are strongly recommended due to the serious concerns related to environmental pollution and insects’ resistance to chemicals. In the present study, we investigated morphological alterations of the ovaries and testes in adult RPW exposed to 2.45 GHz for 5, 15, and 30 s. During these treatments, the relative increase in temperature and the days of survival after irradiation were monitored. Then, RPWs were processed for macroscopical and microscopical analysis. Histological lesions of the ovaries and testes were characterized by the degeneration and necrosis of germinal cells, which increased with the increase in the time of irradiation and the temperature. By the same token, an increase in the temperature of irradiated insects was associated with a decrease in their survival time. These observations lead us to conclude that MWs could represent a useful tool for reducing or eliminating the reproductive capacity of this dreaded insect.
Effect of Fertilization and Planting Date on the Production and Shelf Life of Tuberose
Authors: Castañeda-Saucedo Ma. Claudia , Tapia-Campos Ernesto , Ramirez-Anaya Jessica del Pilar , Barba-Gonzalez Rodrigo , Pita-Lopez Maria Luisa
Published: February 2023

The tuberose, Agave amica, is an ornamental plant appreciated for its oils. The objective of this study was to evaluate the effect of planting dates (April, May and June), dose of NPK (N80-P60-K40, N300-P200-K200, N100-P50-K50 and N00-P00-K00), and fertilizer sources (chemical, organic, combined and control) on tuberose production, flower quality and postharvest shelf life. The physiological variables spike characteristics, leaf color, biomass, and postharvest flower quality were evaluated. The results show that the best planting date is in June; plants planted in June flowered earlier (156 days) and had better flower quality. The fertilization formula N300-P200-K200 produced a higher number of spikes (1.32) and flowers (38.93), a larger stem diameter (0.9 cm), and promoted fewer days to flowering (188d). Plants fertilized with chemical fertilizer had fewer yellow leaves, a larger number of spikes (1.41), a longer spike length (26.89 cm), and a higher number of flowers/spikes (39.28), corms/plants (31.03), and open flowers on the ninth day in vase (13.14) and heavier stems with spikes (134.80 g). In conclusion, the dose of N300-P200-K200 from chemical source and planting in June produced the best flower quality and the shortest production cycle.
Influence of Nitrogen Application Rate on Stem Lodging Resistance Rice under Dry Cultivation
Authors: Wu Meikang , Jiang Hao , Wei Zhiheng , Li Wanchun , Gao Kaiyu , Wang Dongchao , Wei Xiaoshuang , Tian Ping , Cui Jingjing , Di Yuting , Wu Zhihai , Yang Meiying
Published: February 2023

High grain yields of rice (Oryza sativa) under dry cultivation are primarily obtained through high levels of nitrogen (N) input. However, excessive inputs of N increase the risk of lodging. This study aimed to clarify the effect of N application rates on crop morphology, mechanical mechanisms of the stem, and chemical components in the basal stems of rice and their underlying mechanism in association with lodging resistance under dry cultivation. In this study, field experiments on rice were conducted under dry cultivation in early May to early October 2019 and 2020. Six rates of N applied at 0, 70, 140, 210, 280 and 350 kg ha−1 were set at a sowing rate of 150 kg ha−1 with Suijing 18 as the test material. The increased risk of lodging represented by lodging index (LI) and lodging rate with increasing N application was observed under both growing seasons. The plant height, basal internode length, particularly the second internode, and center of gravity height, which positively correlated with the LI, increased significantly with the increase in N application rates. In contrast, internode fullness and carbohydrate content of the basal second (S2) internode, which negatively correlated with LI, decreased significantly with increasing N application rates. A quadratic regression model between N application rates and grain yield showed that better grain yield could be achieved under an N application rate ranging from 210 to 228 kg ha−1. Therefore, the N application rate of (i.e., 210–228 kg N ha−1) could be recommended for the Suijing18 variety under dry cultivation in central Jilin Province for achieving high grain yield and great lodging resistance.
Characteristics and Impacts of Water–Thermal Variation on Grain Yield in the Henan Province, China, on Multiple Time Scales
Authors: Feng Xuefang , Wu Feng , Zai Songmei , Wang Donglin , Zhang Yuzhong , Chai Qihui
Published: February 2023

Water and thermal resources are changing significantly because of climate change, further affecting important crops, such as grains, worldwide. Previous studies on climate change trends and their impacts on grain yield were mainly conducted on a single time scale, with few studies conducted on multiple time scales. Therefore, here, climate data and grain yield statistics from 1978–2021 in the Henan Province were used to assess how water and thermal changes impact grain yield on multiple time scales. Water and thermal variation were analyzed using the least squares method, Mann-Kendall method, and wavelet analysis method, and grain yield impacts were analyzed using gray correlation method. Results showed increasing trends for ≥0 °C and ≥10 °C accumulated temperature and precipitation, with decreased precipitation in spring. The lowest daily minimum temperature increase was 2–3 times the highest daily maximum temperature. Additionally, grain yield fluctuations were caused by climate change. Climate change affected grain yield on all time scales, fluctuating more in autumn than in summer, which was mainly due to changes in temperature followed by precipitation and extreme precipitation. This study provides a scientific basis for the maintenance of food security under climate change.
Assessment of CSM–CERES–Rice as a Decision Support Tool in the Identification of High-Yielding Drought-Tolerant Upland Rice Genotypes
Authors: Hussain Tajamul , Anothai Jakarat , Nualsri Charassri , Ata-Ul-Karim Syed Tahir , Duangpan Saowapa , Hussain Nurda , Ali Awais
Published: February 2023

Drought is considered as one of the critical abiotic stresses affecting the growth and productivity of upland rice. Advanced and rapid identification of drought-tolerant high-yielding genotypes in comparison to conventional rice breeding trials and assessments can play a decisive role in tackling climate-change-associated drought events. This study has endeavored to explore the potential of the CERES–Rice model as a decision support tool (DST) in the identification of drought-tolerant high-yielding upland rice genotypes. Two experiments mentioned as potential experiment (1) for model calibration under optimum conditions and an experiment for yield assessment (2) with three irrigation treatments, (i) a control (100% field capacity [FC]), (ii) moderate stress (70% FC), and (iii) severe stress (50 % FC), were conducted. The results from the yield assessment experiment indicated that the grain yield of the studied genotypes decreased by 24–62% under moderate stress and by 43–78% under severe stress as compared to the control. The values for the drought susceptibility index (DSI) ranged 0.54–1.38 for moderate stress and 0.68–1.23 for severe stress treatment. Based on the DSI and relative yield, genotypes Khao/Sai, Dawk Kham, Dawk Pa–yawm, Goo Meuang Luang, and Mai Tahk under moderate stress and Dawk Kha, Khao/Sai, Nual Hawm, Dawk Pa–yawm, and Bow Leb Nahag under severe stress were among the top five drought-tolerant genotypes as well as high-yielding genotypes. The model accurately simulated grain yield under different irrigation treatments with normalized root mean square error < 10%. An inverse relationship between simulated drought stress indices and grain yield was observed in the regression analysis. Simulated stress indices and water use efficiency (WUE) under different irrigation treatments revealed that the identified drought-tolerant high-yielding genotypes had lower values for stress indices and an increasing trend in their WUE indicating that the model was able to aid in decision support for identifying drought-tolerant genotypes. Simulating the drought stress indices could assist in predicting the response of a genotype under drought stress and the final yield at harvest. The results support the idea that the model could be used as a DST in the identification of drought-tolerant high-yielding genotypes in stressed as well as non-stressed conditions, thus assisting in the genotypic selection process in rice crop breeding programs.
Evaluation of the Effect of Water Stress on Clonal Variations of Cv. Monastrell (Vitis vinifera L.) in South-Eastern Spain: Physiology, Nutrition, Yield, Berry, and Wine-Quality Responses
Authors: Romero Pascual , Botía Pablo , Gil-Muñoz Rocío , del Amor Francisco M. , Navarro Josefa María
Published: February 2023

The present study aims to analyze the physiological and agronomical response to drought among seven local and traditional field-grown Monastrell clones (4, 94, 188, 360, 276, 372, and 373) over four seasons (2018–2021) under optimum irrigation conditions (control) and water stress (stress). We have focussed on measuring Monastrell interclonal variability in plant water relations and leaf gas exchange, vegetative growth, leaf mineral nutrition, yield, water use efficiency (WUE), and grape and wine quality. A classification of the different clones according to drought-tolerance degree and agronomical/oenological performance was established. The classification revealed that (a) The most drought-tolerant clone (i.e., clone 4) was neither the most productive (8600 kg ha−1), the most efficient in terms of water use (average of 25 kg m−3), nor the one that presented a better grape quality; (b) The most productive and efficient clone (i.e., clone 94) (11,566 kg ha−1, average of 30 kg m−3) was also a drought-tolerant clone, but it provided the worst berry and wine qualities with the lowest aromatic/nutraceutical potential, and it is not recommended for premium red wine production; (c) Conversely, clone 360 provided the highest berry quality, but at the expense of a greatly reduced vigor and yield (4000 kg ha−1) and a lower WUEyield (average of 10 kg m−3); (d) Low-vigor clones 372 and 276 were the most sensitive to drought conditions and put more water conservation mechanisms into play, i.e., a tighter control of vine water use and reduced leaf transpiratory surface, under soil water deficit and high vapor pressure deficit (VPD). In addition, these clones reached a balance between drought tolerance, productive water use efficiency (WUEyield), and berry and wine quality, because they provided moderate yields (7400–7700 kg ha−1), a high WUEyield (average between 17–19 kg m−3 applied water), and an enhanced berry and wine quality with greater oenological, nutraceutical, and aromatic potential; (e) High-vigor clone 188 also displayed several mechanisms of drought tolerance (tighter stomatal control of water use), maintained a higher yield (10,500 kg ha−1) and a very high WUEyield (29 kg m−3), enhanced berry quality (similarly to 276 or 372), and improved oenological/aromatic potential, and can also be recommended for the application of low water volume deficit irrigation (DI) strategies under semi-arid conditions.
Yield Components and Development in Indeterminate Tomato Landraces: An Agromorphological Approach to Promoting Their Utilization
Authors: Donoso Adolfo , Salazar Erika
Published: February 2023

Nowadays, increments in tomato yield seem to have reached a plateau. Tomato genebank collections have been recognized as a novel source for yield increments. The use of the diversity in Latin America for novel improved varieties is limited by the knowledge gap regarding field-grown tomatoes. As yield has complex, unresolved trade-offs, agromorphological traits become useful for further improvement. In this study, the development of successive clusters was studied in twenty-four Chilean tomato landraces to elucidate the relationships among agromorphological traits of flowers, inflorescences, and fruits. Plants yielded an average of 3297 g m−2, with a variation coefficient of 0.44. Correlation analyses were performed to evaluate the relationships between yield components and plant phenology. Findings suggested a two-level compensation between average fresh fruit weight and the number of fruits, one on a plant basis and the second on a cluster basis. All traits evaluated had significant phenotypic correlations with yield traits. Growing degree days for a cluster to develop had a low negative phenotypic correlation with yield (−0.33***) and a high genetic correlation with the number of clusters (−0.90***). The number of set flowers, as opposed to the number of flowers, was significantly correlated with average fresh fruit weight (−0.17***), supporting the initiation of the trade-off after the fruit set. This study provides new insight into the plant agromorphology of indeterminate plants. In a global climate change context, further study of trade-off relationships is important for identifying genotypes able to sustain their productivity.
De Novo Assembly of an Allotetraploid Artemisia argyi Genome
Authors: Mei Qiming , Li Hanxiang , Liu Yanbin , Wu Feng , Liu Chuang , Wang Keya , Liu Hongjun , Peng Cheng , Wang Zhengfeng , Wang Long , Liu Zhanfeng , Yan Junhua , Zhang Wei
Published: February 2023

The Chinese mugwort (Artemisia argyi Lév. et Vaniot) is an important traditional Chinese medicine plant that is ubiquitously disturbed in Asia. However, the molecular mechanisms that reflect the natural evolution of Artemisia argyi remain unclear. In this study, a high-quality draft assembly of the allotetraploid A. argyi (ArteW1-Tongbai) was conducted utilizing PacBio long-read sequencing and Hi-C technologies. The assembly is about 7.20 Gb with a contig N50 length of 0.87 Mb. The allotetraploid genome of ArteW1-Tongbai is highly heterozygous and rich in repeat sequences (the heterozygous ratio is 1.36%, and the repeat rate is 86.26%). A total of 139,245 protein-coding genes were identified. The KEGG enrichment analysis revealed that 846 species-specific genes were related to the biosynthesis of secondary metabolites. The plants with allopolyploid genomes can potentially exhibit a better adaptive capacity to environmental stresses and accumulation of secondary metabolites. Therefore, the genome assembly serves as a valuable reference for Artemisia, the genus characterized by species richness and diverse specialized metabolites.
Application of Bacillus subtilis for the Alleviation of Salinity Stress in Different Cultivars of Wheat (Tritium aestivum L.)
Authors: Gul Saboor , Javed Sadia , Azeem Muhammad , Aftab Amreen , Anwaar Nazima , Mehmood Tahir , Zeshan Basit
Published: February 2023

Salinity has a negative impact on the agricultural production of crops. It adversely affects the physiochemical properties of the soil and ecological balance of the area. Plant growth-promoting bacteria play a key role in the biological control of phyto-pathogens and abiotic stress including salinity. Four varieties of wheat crop (V1: Akbar 2019, V2: Dilkash 2021, V3: Faisalabad 2008, and V4: Subhani 2020) were compared for their salinity stress tolerance and response towards Bacillus subtilis NA2. A completely randomized design (4 wheat varieties × 3 salt stress levels × 3 replicate × 2 control and bacterial treatments = 72 pots) was adopted using distilled water as a control. Stress negatively affected the plant growth. However, plants primed with Bacillus subtilis NA2 showed improved growth (plant lengths 29.45% and increased biomass 33.23%). Overall, bacterial strain enhanced the levels of carotenoids (45.53%), anthocyanin (32.51%), ascorbic acid (41.53%), total soluble proteins (59.21%), chlorophyll contents (49.65%), and peroxidase activity (31.76%). Levels of malondialdehyde (27.42%) and hydrogen peroxide (20.37%), catalase (16.48%), and ascorbate peroxidase (19.24%) decreased. With commensurable benefits, it can be inferred from the above study that the Bacillus subtilis NA2 strain is beneficial for the better yield of wheat under salinity stress by improving the plant defense mechanism and may be adopted in future by farmers.
Sustainability and the Thoroughbred Breeding and Racing Industries: An Enhanced One Welfare Perspective
Authors: Stallones Lorann , McManus Phil , McGreevy Paul
Published: February 2023

As society debates the use of animals in sport, entertainment, and leisure, there is an increasing focus on the welfare, social, and ecological impacts of such activities on the animals, human participants, people close to them, and the physical environment. This article introduces the “Enhanced One Welfare Framework” to reveal significant costs and benefits associated with Thoroughbred breeding and racing globally. In addition, relative to calls to ban horseracing and similar activities as part of sustainability approaches that focus chiefly on animals, the “Enhanced One Welfare Framework” is better positioned politically to guide discussions that renegotiate the conditions under which horses are used for sport and the impact racing has on humans and the planet. In 2020, the International Federation of Horseracing Authorities issued its minimum horse welfare standards based on the Five Domains model, positioning lifelong horse welfare as “fundamentally important to the viability and sustainability of the industry”. In this article, we critique the One Welfare framework’s historic lack of focus on sport and enhance it by including sport, leisure, and entertainment and framing it within the Five Domains model. We offer a novel extension of the Five Domains model beyond animal welfare to consider human welfare and the physical environmental impacts of the sport, leisure, and entertainment industries and propose innovations that may help thoroughbred breeding and racing assure a sustainable future.
Importance of Foot and Leg Structure for Beef Cattle in Forage-Based Production Systems
Authors: Sitz Taylre , DelCurto-Wyffels Hannah , Van Emon Megan , Wyffels Sam , Retallick Kelli , Tarpoff Esther , Kangas Kurt , DelCurto Tim
Published: February 2023

Understanding the relationship of foot angle and claw set to beef cattle structural soundness will be critical to the selection of animals that fit forage-based production systems. In an effort to address concerns about foot and leg structure, the American Angus Association’s foot angle and foot claw set expected progeny differences (EPD) were developed in 2019. As a result, these relatively new EPD and associated guidelines have limited phenotypic data submitted thus far. While ample research has evaluated lameness and foot issues in the dairy breeds, less is known about the factors that affect foot structure in beef cattle. This review focuses on beef cattle foot and leg structure, selection factors that may have led to increased problems with feet and legs, and the importance of foot and leg structure in forage-based grazing production systems. Specifically, the importance of locomotion and freedom of movement in extensive rangeland environments is discussed relative to the current literature. In addition, environmental factors that may influence foot and leg structure are addressed as well as heritability of various aspects of foot and leg traits. Where possible, information gaps and research needs are identified to enhance further investigation and the improvement of foot and leg selection tools.
Estimation of the Genetic Components of (Co)variance and Preliminary Genome-Wide Association Study for Reproductive Efficiency in Retinta Beef Cattle
Authors: Jiménez José María , Morales Rosa María , Menéndez-Buxadera Alberto , Demyda-Peyrás Sebastián , Laseca Nora , Molina Antonio
Published: February 2023

In this study, we analyzed the variation of reproductive efficiency, estimated as the deviation between the optimal and real parity number of females at each stage of the cow’s life, in 12,554 cows belonging to the Retinta Spanish cattle breed, using classical repeatability and random regression models. The results of the analyses using repeatability model and the random regression model suggest that reproductive efficiency is not homogeneous throughout the cow’s life. The h2 estimate for this model was 0.30, while for the random regression model it increased across the parities, from 0.24 at the first calving to 0.51 at calving number 9. Additionally, we performed a preliminary genome-wide association study for this trait in a population of 252 Retinta cows genotyped using the Axiom Bovine Genotyping v3 Array. The results showed 5 SNPs significantly associated with reproductive efficiency, located in two genomic regions (BTA4 and BTA28). The functional analysis revealed the presence of 5 candidate genes located within these regions, which were previously involved in different aspects related to fertility in cattle and mice models. This new information could give us a better understanding of the genetic architecture of reproductive traits in this species, as well as allow us to accurately select more fertile cows.
Fecal Microbiota, Bile Acids, Sterols, and Fatty Acids in Dogs with Chronic Enteropathy Fed a Home-Cooked Diet Supplemented with Coconut Oil
Authors: Vecchiato Carla Giuditta , Pinna Carlo , Sung Chi-Hsuan , Borrelli De Andreis Francesca , Suchodolski Jan S. , Pilla Rachel , Delsante Costanza , Sportelli Federica , Mammi Ludovica Maria Eugenia , Pietra Marco , Biagi Giacomo
Published: February 2023

Medium-chain fatty acids (MCFAs) are considered to be interesting energy sources for dogs affected by chronic enteropathies (CE). This study analyzed the clinical scores, fecal microbiota, and metabolomes of 18 CE dogs fed a home-cooked diet (HCD) supplemented with virgin coconut oil (VCO), a source of MCFA, at 10% of metabolizable energy (HCD + VCO). The dogs were clinically evaluated with the Canine Chronic Enteropathy Activity Index (CCECAI) before and at the end of study. Fecal samples were collected at baseline, after 7 days of HCD, and after 30 days of HCD + VCO, for fecal score (FS) assessment, microbial analysis, and determination of bile acids (BA), sterols, and fatty acids (FA). The dogs responded positively to diet change, as shown by the CCECAI improvement (p = 0.001); HCD reduced fecal fat excretion and HCD + VCO improved FS (p < 0.001), even though an increase in fecal moisture occurred due to HCD (p = 0.001). HCD modified fecal FA (C6:0: +79%, C14:0: +74%, C20:0: +43%, C22:0: +58%, C24:0: +47%, C18:3n−3: +106%, C20:4n−6: +56%, and monounsaturated FA (MUFA): −23%, p < 0.05) and sterol profile (coprostanol: −27%, sitostanol: −86%, p < 0.01). VCO increased (p < 0.05) fecal total saturated FA (SFA: +28%, C14:0: +142%, C16:0 +21%, C22:0 +33%) and selected MCFAs (+162%; C10:0 +183%, C12:0 +600%), while reducing (p < 0.05) total MUFA (−29%), polyunsaturated FA (−26%), campesterol (−56%) and phyto-/zoosterols ratio (0.93:1 vs. 0.36:1). The median dysbiosis index was <0 and, together with fecal BA, was not significantly affected by HCD nor by VCO. The HCD diet increased total fecal bacteria (p = 0.005) and the abundance of Fusobacterium spp. (p = 0.028). This study confirmed that clinical signs, and to a lesser extent fecal microbiota and metabolome, are positively influenced by HCD in CE dogs. Moreover, it has been shown that fecal proportions of MCFA increased when MCFAs were supplemented in those dogs. The present results emphasize the need for future studies to better understand the intestinal absorptive mechanism of MCFA in dogs.
Effects of Dietary Supplementation of Chinese Yam Polysaccharide on Carcass Composition, Meat Quality, and Antioxidant Capacity in Broilers
Authors: Chang Yadi , Zhang Jinzhou , Jin Yan , Deng Jiahua , Shi Mingyan , Miao Zhiguo
Published: February 2023

The study aimed to evaluate the influences of the dietary supplementation of Chinese yam polysaccharide (CYP) on the carcass performance, antioxidant capacity, and meat quality of broilers. Three hundred and sixty healthy 1-day-old broilers with similar body weight (39 ± 1 g, gender balanced) were randomly divided into four groups (control, CYP1, CYP2, and CYP3 groups). In the control group, broilers were fed a basal diet with CYP, and the CYP1, CYP2, and CYP3 groups were fed diets supplemented with 250, 500, and 1000 mg/kg CYP, respectively. There were three replicates in each group, 30 birds in each replicate, and the feeding trial lasted for 48 days. Statistical analysis was performed using SPSS 17.0 (SPSS Inc., Chicago, IL, USA) by one-way analysis of variance. The results showed that compared with the control group, dietary supplementation with 500 mg/kg CYP can improve live weight, half-eviscerated carcass percentage, eviscerated carcass percentage, and thigh muscle percentage. Moreover, dietary supplementation with 500 mg/kg CYP can improve the contents of total antioxidant capacity (T-AOC), total superoxide dismutase (T-SOD), glutathione peroxidase (GPX), and glutathione s-transferase (GST) in serum (p < 0.05). Meanwhile, the mRNA expression levels of nuclear factor-erythroid 2-related factor 2 (Nrf2), heme oxygenase 1 (HO-1), quinone oxidoreductase (NQO1), superoxide dismutase 1 (SOD1), glutathione peroxidase 1 (GPX1), and catalase (CAT) in the liver; the mRNA expression levels of HO-1, NQO1, GPX1, and CAT in the breast muscle; and the mRNA expression levels of NQO1, SOD1, and CAT in the thigh muscle of broilers in the CYP2 group were significantly increased (p < 0.05). In addition, the yellowness and shear force of the thigh and breast muscles and the content of malondialdehyde (MDA) in the serum of broilers in the control group were higher than that in the CYP2 groups (p < 0.05). The results demonstrated that the CYP2 group had the best effect on improving meat quality. In conclusion, dietary supplementation with 500 mg/kg CYP can improve the meat quality of broilers by improving carcass quality, meat color, shear force, and antioxidant capacity.
Confirmation of the Prognostic Value of Foxp3+ Cells in Canine Mammary Tumors
Authors: Parisi Francesca , Millanta Francesca , Nicastro Marika , Vannozzi Iacopo , Poli Alessandro
Published: February 2023

Foxp3+ cell counts were evaluated by immunohistochemistry in 59 canine mammary tumors, 20 adenomas, and 39 carcinomas in three different compartments: intratumoral, within the adjacent stroma, and in the distant stroma. Foxp3+ lymphocyte counts were compared with histotype, grading, presence of lymphatic invasion, immunohistochemical expression of estrogen and progesterone receptors, expression of c-erbB-2, and the overall survival (OS). Our findings confirmed that Foxp3+ cells were significantly higher in canine mammary carcinomas compared to adenomas. A significantly higher number of Foxp3+ cells were detected in grade III carcinomas compared to grade II carcinomas, as well as in tumors with lymphatic invasion and loss of ER-expression. Finally, a high number of Foxp3+ cells was associated with poor prognosis. In conclusion, our findings highlighted the association of Foxp3+ lymphocytes with negative clinicopathological features and shorter overall survival (OS), thus confirming the role of Tregs as a negative prognostic marker in canine mammary carcinomas.
Expression Profile of miRNA from High, Middle, and Low Stress-Responding Sheep during Bacterial Endotoxin Challenge
Authors: Shandilya Umesh K. , Sharma Ankita , Naylor Danielle , Canovas Angela , Mallard Bonnie , Karrow Niel A.
Published: February 2023

Animals respond to stress by activating a wide array of physiological and behavioral responses that are collectively referred to as the stress response. MicroRNAs (miRNAs) are small, noncoding RNAs that play key roles in the regulation of homeostasis. There are many reports demonstrating examples of stress-induced miRNA expression profiles. The aim of this study was to determine the circulatory miRNA profile of variable stress-responding lambs (n = 112) categorized based on their cortisol levels as high (HSR, 336.2 ± 27.9 nmol/L), middle (MSR, 147.3 ±9.5 nmol/L), and low (LSR, 32.1 ± 10.4 nmol/L) stress responders post-LPS challenge (400 ng/kg iv). Blood was collected from the jugular vein at 0 (T0) and 4 h (T4) post-LPS challenge, and miRNAs were isolated from four animals from each group. An array of 84 miRNAs and 6 individual miRNAs were evaluated using qPCR. Among 90 miRNAs, there were 48 differentially expressed (DE) miRNAs (log fold change (FC) > 2 < log FC) in the HSR group, 46 in the MSR group, and 49 in the LSR group compared with T0 (control) samples. In the HSR group, three miRNAs, miR-485-5p, miR-1193-5p, and miR-3957-5p were significantly (p < 0.05) upregulated, while seven miRNAs, miR-376b-3p, miR-376c-3p, miR-411b-5p, miR-376a-3p, miR-376b-3p, miR-376c-3p, and miR-381-3p, were downregulated (p < 0.05) as compared to the LSR and MSR groups. Functional analysis of DE miRNAs revealed their roles in Ras and MAPK signaling, cytokine signaling, the adaptive immune system, and transcription pathways in the HSR phenotype, implicating a hyper-induced acute-phase response. In contrast, in the LSR group, enriched pathways included glucagon signaling metabolic regulation, the transportation of amino acids and ions, and the integration of energy metabolism. Taken together, these results indicate variation in the acute-phase response to an immune stress challenge, and these miRNAs are implicated in regulating responses within cortisol-based phenotypes.
Diclofenac and Meloxicam Exhibited Anti-Virulence Activities Targeting Staphyloxanthin Production in Methicillin-Resistant Staphylococcus aureus
Authors: Elmesseri Rana A. , Saleh Sarra E. , Ghobish Sarah A. , Majrashi Taghreed A. , Elsherif Heba M. , Aboshanab Khaled M.
Published: February 2023

Staphylococcus aureus (S. aureus) is a worldwide leading versatile pathogen that causes a wide range of serious infections. The emergence of antimicrobial resistance against S. aureus resulted in an urgent need to develop new antimicrobials in the new era. The methicillin-resistant S. aureus (MRSA) prevalence in hospital and community settings necessitates the discovery of novel anti-pathogenic agents. Staphyloxanthin (STX) is a key virulence factor for the survival of MRSA against host innate immunity. The current work aimed to demonstrate the anti-virulence properties of meloxicam (MXM) as compared to diclofenac (DC), which was previously reported to mitigate the virulence of multidrug-resistant Staphylococcus aureus and test their activities in STX production. A total of 80 S. aureus clinical isolates were included, wherein a qualitative and quantitative assessment of STX inhibition by diclofenac and meloxicam was performed. The quantitative gene expression of STX biosynthetic genes (crtM, crtN and sigB) and hla (coded for α-hemolysin) as a virulence gene with and without DC and MXM was conducted, followed by molecular docking analysis for further confirmation. DC and MXM potently inhibited the synthesis of STX at 47 and 59 µg/mL to reach 79.3–98% and 80.6–96.7% inhibition, respectively. Treated cells also revealed a significant downregulation of virulence genes responsible for STX synthesis, such as crtM, crtN and global transcriptional regulator sigB along with the hla gene. Furthermore, computational studies unveiled strong interactions between the CrtM binding site and DC/MXM. In conclusion, this study highlights the potential role and repurposing of DC and MXM as adjuvants to conventional antimicrobials and as an anti-virulent to combat MRSA infections.
Mutations Related to Antibiotics Resistance in Helicobacter pylori Clinical Isolates from Bangladesh
Authors: Fauzia Kartika Afrida , Aftab Hafeza , Tshibangu-Kabamba Evariste , Alfaray Ricky Indra , Saruuljavkhlan Batsaikhan , Cimuanga-Mukanya Alain , Matsumoto Takashi , Subsomwong Phawinee , Akada Junko , Miftahussurur Muhammad , Yamaoka Yoshio
Published: February 2023

Current management of gastric inflammation involves the eradication of Helicobacter pylori. However, the effectiveness of commonly used antibiotics against H. pylori infection has decreased due to antibiotic resistance. Phenotypic-based diagnostics are laborious and finding the cause of resistance can be difficult. Therefore, early detection and understanding of the underlying mechanism of this resistance are necessary. This study evaluated the mutations in the genes related to the Antimicrobial Resistance (AMR) of the clinical isolates from Bangladeshi subjects. Whole-genome sequencing was performed on 56 isolates and the genes (such as pbp1a, rdxA, ribF, fur, gyrA, gyrB, 23S rRNA, and infB) were extracted. The reads were assembled, and the SNPs were extracted by the latest pipeline for antibiotic mutation analysis, ARIBA. The mutations and the association with the antibiotic phenotypes were evaluated using Fisher’s exact test. In this study, the clarithromycin resistance rate was high, 39.3% (22/56), with the median MIC 24 mg/L ranging from 2 to 128 mg/L. The mutation of A2147G was significantly associated with resistance (p = 0.000018) but not in locus A2146G (p = 0.056). Levofloxacin also posed a high resistance. We observed that the mutation of D91N (but not D91Y) (p = 0.002) and N87K (p = 0.002) of gyrA was associated with levofloxacin resistance. Mutations in locus A343V (p = 0.041) of gyrB also showed a significant association. Meanwhile, in the pbp1a gene, several mutations might explain the resistance; they were G594fs (p = 0.036), K306R (p = 0.036), N562Y (p = 0.0006), and V45I (p = 0.018). The prevalence of metronidazole was exceptionally high (96.4%), and numerous mutations occurred in rdxA genes, including the truncation of genes. These results imply that the mutation in genes encoding the target protein of antibiotics remains the critical resistance mechanism in H. pylori.
Assessing the Clinical Characteristics and Management of COVID-19 among Pediatric Patients in Ghana: Findings and Implications
Authors: Sefah Israel Abebrese , Sarkodie Seth Adade , Pichierri Giuseppe , Schellack Natalie , Godman Brian
Published: February 2023

There is an increasing focus across countries on researching the management of children admitted to hospital with COVID-19. This stems from an increasing prevalence due to new variants, combined with concerns with the overuse of antimicrobials driving up resistance rates. Standard treatment guidelines (STGs) have been produced in Ghana to improve their care. Consequently, there is a need to document the clinical characteristics of children diagnosed and admitted with COVID-19 to our hospital in Ghana, factors influencing compliance to the STG and treatment outcomes. In all, 201 patients were surveyed between March 2020 and December 2021, with males accounting for 51.7% of surveyed children. Those aged between 6 and 10 years were the largest group (44.8%). Nasal congestion and fever were some of the commonest presenting complaints, while pneumonia was the commonest (80.6%) COVID-19 complication. In all, 80.0% of all admissions were discharged with no untreated complications, with a 10.9% mortality rate. A combination of azithromycin and hydroxychloroquine (41.29%) was the most prescribed antimicrobial regimen. Compliance to the STG was variable (68.2% compliance). Increased compliance was associated with a sore throat as a presenting symptom. Mortality increased following transfer to the ICU. However, current recommendations to prescribe antimicrobials without demonstrable bacterial or fungal infections needs changing to reduce future resistance. These are areas to address in the future.
Phage Therapy as an Alternative Treatment Modality for Resistant Staphylococcus aureus Infections
Authors: Atshan Salman Sahab , Hamat Rukman Awang , Aljaberi Musheer A. , Chen Jung-Sheng , Huang Shih-Wei , Lin Chung-Ying , Mullins Benjamin J. , Kicic Anthony
Published: February 2023

The production and use of antibiotics increased significantly after the Second World War due to their effectiveness against bacterial infections. However, bacterial resistance also emerged and has now become an important global issue. Those most in need are typically high-risk and include individuals who experience burns and other wounds, as well as those with pulmonary infections caused by antibiotic-resistant bacteria, such as Pseudomonas aeruginosa, Acinetobacter sp, and Staphylococci. With investment to develop new antibiotics waning, finding and developing alternative therapeutic strategies to tackle this issue is imperative. One option remerging in popularity is bacteriophage (phage) therapy. This review focuses on Staphylococcus aureus and how it has developed resistance to antibiotics. It also discusses the potential of phage therapy in this setting and its appropriateness in high-risk people, such as those with cystic fibrosis, where it typically forms a biofilm.
Antibiotic-Loaded Coatings to Reduce Fracture-Related Infections: Retrospective Case Series of Patients with Increased Infectious Risk
Authors: De Meo Daniele , Cera Gianluca , Pica Roberta , Perfetti Fabiano , Martini Paolo , Perciballi Beatrice , Ceccarelli Giancarlo , Persiani Pietro , Villani Ciro
Published: February 2023

Local antibiotic delivery strategies have been increasingly employed for the prevention of fracture-related infections (FRIs). The aim of this study is to evaluate the efficacy and safety of antibiotic-coated implants in the prevention of FRIs after surgical treatment in patients with increased infectious risk. A retrospective observational study has been conducted on patients with upper and lower limb fractures treated with internal fixation or prosthetic replacements, using a gentamicin coated nail (CN) and/or antibiotic-loaded hydrogel applied to the implant of choice (ALH). The study included 37 patients (20 M, 17 F), with a mean age of 63 years. The mean estimated preoperative infectious risk score was 6.4%. ALH was used in 27 cases, tibial CNs were implanted in 4 cases, and both were employed in 6 cases. The antibiotics used locally were gentamicin in 72.97% of cases (27 patients) and a combination of gentamicin + vancomycin in 27.03% of cases (10 patients). Mean follow-up was 32 months. Only one case (2.94%) showed onset of FRI at 5 months after surgery. Local antibiotic prophylaxis by coating resulted in a reduction in the incidence FRI, as compared to the estimated preoperative risk. The use of ALH allows for the choice of antibiotic; however, the application of antibiotics seems more nonuniform when applied to a nail.
Molecular and Antimicrobial Susceptibility Characterization of Escherichia coli Isolates from Bovine Slaughterhouse Process
Authors: Vázquez-Villanueva José , Vázquez Karina , Martínez-Vázquez Ana Verónica , Wong-González Alfredo , Hernández-Escareño Jesus , Cabrero-Martínez Omar , Cruz-Pulido Wendy Lizeth , Guerrero Abraham , Rivera Gildardo , Bocanegra-García Virgilio
Published: February 2023

Antimicrobials are routinely used in human and veterinary medicine. With repeated exposure, antimicrobials promote antibiotic resistance, which poses a threat to public health. In this study, we aimed to determine the susceptibility patterns, virulence factors, and phylogroups of E. coli isolates during the killing process in a bovine slaughterhouse. We analyzed 336 samples (from water, surfaces, carcasses, and feces), and 83.3% (280/336) were positive for E. coli. The most common phenotypic resistances that we detected were 50.7% (142/280) for tetracycline, 44.2% (124/280) for cephalothin, 34.6% (97/280) for streptomycin, and 36.7% (103/280) for ampicillin. A total of 82.4% of the isolates had resistance for at least one antimicrobial, and 37.5% presented multiresistance. We detected a total of 69 different phenotypic resistance patterns. We detected six other resistance-related genes, the most prevalent being tetA (22.5%) and strB (15.7%). The prevalence values of the virulence genes were 5.4% in hlyA, 1.4% in stx1, and 0.7% in stx2. The frequencies of the pathogenic strains (B2 and D) were 32.8% (92/280) and 67.1% (188/280) as commensals A and B1, respectively. E. coli isolates with pathogenic potential and multiresistance may represent an important source of dissemination and a risk to consumers.
Therapeutic Potential of an Azithromycin-Colistin Combination against XDR K. pneumoniae in a 3D Collagen-Based In Vitro Wound Model of a Biofilm Infection
Authors: Moshynets Olena V. , Baranovskyi Taras P. , Iungin Olga S. , Krikunov Alexey A. , Potochilova Viktoria V. , Rudnieva Kateryna L. , Potters Geert , Pokholenko Ianina
Published: February 2023

A therapeutic combination of azithromycin (AZM) and colistin methanesulfonate (CMS) was shown to be effective against both non-PDR and PDR Klebsiella pneumoniae biofilms in vitro. These anti-biofilm effects, however, may not correlate with effects observed in standard plate assays, nor will they representative of in vivo therapeutic action. After all, biofilm-associated infection processes are also impacted by the presence of wound bed components, such as host cells or wound fluids, which can all affect the antibiotic effectiveness. Therefore, an in vitro wound model of biofilm infection which partially mimics the complex microenvironment of infected wounds was developed to investigate the therapeutic potential of an AZM-CMS combination against XDR K. pneumoniae isolates. The model consists of a 3D collagen sponge-like scaffold seeded with HEK293 cells submerged in a fluid milieu mimicking the wound bed exudate. Media that were tested were all based on different strengths of Dulbecco’s modified Eagles/high glucose medium supplemented with fetal bovine serum, and/or Bacto Proteose peptone. Use of this model confirmed AZM to be a highly effective antibiofilm component, when applied alone or in combination with CMS, whereas CMS alone had little antibacterial effectiveness or even stimulated biofilm development. The wound model proposed here proves therefore, to be an effective aid in the study of drug combinations under realistic conditions.
Food Wastes and Microalgae as Sources of Bioactive Compounds and Pigments in a Modern Biorefinery: A Review
Authors: Martins Rodrigo , Sales Hélia , Pontes Rita , Nunes João , Gouveia Isabel
Published: February 2023

The United Nations 2030 Agenda for Sustainable Development has created more pressure on countries and society at large for the development of alternative solutions for synthetic and fossil fuel derived products, thus mitigating climate change and environmental hazards. Food wastes and microalgae have been studied for decades as potential sources of several compounds that could be employed in various fields of application from pharmaceutical to textile and packaging. Although multiple research efforts have been put towards extracting rich compounds (i.e., phenolic compounds, tocopherols, and tocotrienols) from these sources, they still remain overlooked as two major sources of bioactive compounds and pigments, mainly due to inefficient extraction processes. Hence, there is a growing need for the development of optimized extraction methods while employing non-organic solvent options following the main principles of green chemistry. This review will focus on delivering a clear and deep analysis on the existing procedures for obtaining bioactive compounds and pigments from food wastes derived from the most consumed and produced fruit crops in the world such as apples, oranges, cherries, almonds, and mangoes, and microalgal biomass, while giving light to the existing drawbacks in need to be solved in order to take full advantage of the rich properties present in these two major biorefinery sources.
BMP-7 Attenuates Sarcopenia and Adverse Muscle Remodeling in Diabetic Mice via Alleviation of Lipids, Inflammation, HMGB1, and Pyroptosis
Authors: Narasimhulu Chandrakala Aluganti , Singla Dinender K.
Published: February 2023

Diabetic myopathy involves hyperglycemia, oxidative stress, and inflammation. However, the role of hypercholesterolemia-induced inflammation-mediated pathological mechanisms leading to fibrosis, sarcopenia, deterioration of muscle, and muscle dysfunction in diabetes is not well understood. In this study, we investigated the novel role of bone morphogenetic protein-7 (BMP-7) in ameliorating metabolic alterations, inflammation, pyroptosis, TGF-β/SMAD cell signaling mechanisms, and progression of diabetic myopathy. C57BL/6J mice were treated with saline, streptozotocin (STZ), or STZ+BMP-7. Diabetes was confirmed by increased fasting glucose levels and a glucose tolerance test. Gastrocnemius muscle and blood samples were collected for lipid and tissue analysis using various methods. A significant increase in hyperglycemia resulted in an increase in lipid accumulation, monocyte infiltration, and inflammation, as well as an increase in pyroptotic markers and signaling markers in diabetic muscle myocytes. A structural analysis showed significant muscle loss, and increased muscle deterioration and fibrosis leading to muscle dysfunction. BMP-7 attenuated pathological processes that resulted in significantly improved muscle function. We report, for the first time, that increased hyperlipidemia aggravates inflammation-induced pyroptosis, resulting in significant muscle loss, sarcopenia, and adverse skeletal muscle remodeling in diabetic muscle myopathy. Interventional treatment with BMP-7 attenuates hypercholesterolemia-induced inflammation-mediated sarcopenia and adverse muscle remodeling, suggesting BMP-7 could be a potential treatment option for diabetic muscle myopathy.