bluuebunny/crossref_metadata_2025 huggingface dataset:
Formation of ZnO/CuO Heterostructures Based on Quasi-One-Dimensional Nanomaterials
Authors: Murzin Serguei P.
Published: December 2022

Nanostructured metal oxides are of great interest both for advanced research and for a wide range of applications that contribute to the increasing demands of electronics, photonics, catalysis, sensorics, and other high-tech industries and are being actively researched and developed. One-dimensional nanocrystal arrays of copper and zinc oxides have become prominent in optoelectronic devices and energy conversion systems. However, although desirable improved properties have been demonstrated, the morphology of materials containing copper and zinc oxide nanowires is extremely sensitive to synthesis conditions and difficult to control. Studies focused on the morphology control of such quasi-one-dimensional materials are not numerous, so the consideration of this issue is still relevant. The characteristics of devices based on such oxide materials can be improved by taking advantage of nanoheterojunctions. A special feature is the possibility of forming a polycrystalline heterojunction in a system of semiconductors belonging to different crystalline syngonies. Currently, much attention is devoted to developing reliable methods of obtaining such nanomaterials, including those, based on processes exploiting novel physical effects. Possibilities of synthesis by pulse-periodic laser irradiation of arrays of quasi-one-dimensional ZnO nanostructures with varying micromorphology on metallic substrates, as well as the creation of ZnO/CuO heterostructures based on ZnO nanowires, were considered. The main distinguishing feature of this approach was the use of laser-induced vibrations to intensify diffusion processes in the solid phase of metallic materials as compared to the simple effects of laser beam heating. Expanding the area of application of the advanced method of creating oxide heterostructures requires a detailed and comprehensive study of new possibilities used to form structures with improved physical properties.
Depth of Bacterial Penetration into Dentinal Tubules after Use of Different Irrigation Solutions: A Systematic Review of In Vitro Studies
Authors: Tsesis Igor , Lokshin Michal , Littner Dan , Goldberger Tomer , Rosen Eyal
Published: December 2022

(1) The objective of the study is to conduct a comprehensive systematic review of in vitro studies in order to assess the depth to which E. faecalis bacteria penetrate human dentinal tubules after the use of various irrigation solutions. (2) Methods: A literature search of the MEDLINE, Scopus, Cochrane CENTRAL, and Embase databases was conducted, as well as a backward and forward citation search. Two independent reviewers then selected suitable studies based on inclusion and exclusion criteria. Data were extracted and the risk of bias and methodology of the studies were evaluated. (3) Results: Out of a total of 504 papers evaluated following the removal of duplicates, 7 studies met the inclusion criteria and were included in the systematic review. The heterogeneity of the studies made it impossible to perform a meta-analysis. The majority of the studies reported that sodium hypochlorite (NaOCl) and chlorhexidine digluconate (CHX) can affect the penetration depth of E. faecalis suspensions. The studies included in this review possess a moderate to high risk of bias and thus represent moderate evidence that the antimicrobial activity of NaOCl and CHX affects the intra-tubular penetration of bacteria. (4) Conclusions: The evidence indicates that irrigants may affect the bacteria inside human dentinal tubules. Standardized high-quality methods are needed to evaluate bacterial penetration in in vitro studies.
Reducing Mercury Emission Uncertainty from Artisanal and Small-Scale Gold Mining Using Bootstrap Confidence Intervals: An Assessment of Emission Reduction Scenarios
Authors: Bruno Delia Evelina , De Simone Francesco , Cinnirella Sergio , Hedgecock Ian Michael , D’Amore Francesco , Pirrone Nicola
Published: December 2022

Atmospheric mercury emission scenarios from artisanal and small-scale gold mining for 56 tropical and subtropical countries have been elaborated and assessed for their comparative significance. A multi-step quantitative method that yields narrow and robust confidence intervals for mercury emission estimates was employed. Firstly, data on gold production for different years, the ratio of mercury used in the different amalgamation processes, and ancillary input parameters were retrieved from official and unofficial sources, and their potential for emission reduction examined. Then, a Monte Carlo method to combine the data and generate mercury emission samples was used. These samples were processed by a non-parametric re-sampling method (bootstrap) to obtain robust estimates of mercury emissions, and their 95% confidence intervals, both for the current state and for the emission scenarios designed in this study. The artisanal and small-scale gold mining mercury emission (to the atmosphere) estimates agree with those reported in the Global Mercury Assessment 2018; however, the overall uncertainty is reduced from roughly 100% in the Global Mercury Assessment (779.59 tons/y; uncertainty range: 361.07–1197.97) to 27% (1091.93 tons/y; confidence interval at 95% level of confidence: 964.54–1219.77) in this study. This is a substantial outcome since the narrowing of the confidence intervals permits a more meaningful evaluation of the different emission scenarios investigated, which otherwise, given the broad uncertainty of other estimates, would have led only to vague conclusions in a study of this nature.
Optimization of Discharging Electrodes of a Multi-Chamber Electrostatic Precipitator for Small Heat Sources
Authors: Holubčík Michal , Drga Juraj , Čajová Kantová Nikola , Najser Jan , Frantík Jaroslav
Published: December 2022

On the way to reducing emissions released into the atmosphere, there is an obstacle in the form of the emissions of solid pollutants produced by households, namely the burning of solid fuels in small heat sources. In this article, the authors deal with the development of a low-cost electrostatic precipitator, which would be able to significantly reduce the production of particulate matter. This is a tubular precipitator concept, which is enhanced by dividing the precipitation space into four chambers, each of which has an ionization electrode. With the investigated structural arrangement, it is possible to increase the size of the collection area without affecting the external dimensions of the separator. The essence of this article was to focus on the design of an ionization electrode, which, in addition to the function of a negative electrode, would also fulfill the function of a structural element of the proposed geometry. The work contains a technical design for the shape of the ionization electrode, which was subsequently examined using ANSYS Fluent software. The conditions under which a corona discharge will occur on the electrodes and how particulate matter is captured in the separation device were investigated with the help of simulations of the electric field intensity. According to the achieved simulation results, calculations were made for the theoretical efficiency of particle collection, which reached a value of approximately 78%.
Ambient Nanoparticles (PM0.1) Mapping in Thailand
Authors: Phairuang Worradorn , Piriyakarnsakul Suthida , Inerb Muanfun , Hongtieab Surapa , Thongyen Thunyapat , Chomanee Jiraporn , Boongla Yaowatat , Suriyawong Phuchiwan , Samae Hisam , Chanonmuang Phuvasa , Suwattiga Panwadee , Chetiyanukornkul Thaneeya , Panyametheekul Sirima , Amin Muhammad , Hata Mitsuhiko , Furuuchi Masami
Published: December 2022

Nanoparticles (NPs), nanoaerosols (NAs), ultrafine particles (UFPs), and PM0.1 (diameters ≤ 0.1 µm or 100 nm) are used interchangeably in the field of atmospheric studies. This review article summarizes recent research on PM0.1 in Thailand. The review involved peer-reviewed papers that appeared in the Scopus and the Web of Science databases and included the most recently published articles in the past 10 years (2013–2022). PM0.1 mainly originate from combustion processes such as in motor vehicles. The highest mass concentration of PM0.1 occurs during the dry season, in which open fires occur in some regions of Thailand. The northern area of the country has higher PM0.1 mass concentrations, followed by the central and southern areas. Carbonaceous nanoaerosols are produced during normal periods, and the proportions of organic to elemental carbon and char to soot suggest that these originate from motor vehicles. However, in haze periods, biomass fires can also produce carbon-containing particles. PM0.1 pollution from local and cross-border countries also needs to be considered. The overall conclusions reached will likely have a beneficial long-term impact on achieving a blue sky over Thailand through the development of coherent policies and managing new air pollution challenges and sharing knowledge with a broader audience.
Change in Population Exposure to Future Tropical Cyclones in Northwest Pacific
Authors: Qin Lianjie , Liao Xinli , Xu Wei , Meng Chenna , Zhai Guangran
Published: December 2022

The impact of tropical cyclones is expected to worsen with continued global warming and socioeconomic development. Quantifying population exposure to strong winds and heavy rainfall induced by tropical cyclones is a core element of tropical cyclone population risk assessment. Based on the demographic dataset of Shared Socioeconomic Pathways and future tropical cyclone data, we first calculate and analyze the changes in impact frequency and population exposure to four tropical cyclone scenarios in the Northwest Pacific over the period 2015–2050. Then, we quantitatively assess the contribution rates of climate change, population change, and their joint change to population exposure change. The results show that East China, South China, and Southeast China are the areas with high exposure change. Additionally, most of the high exposure changes (absolute changes over 400,000 people) are significant, and primarily influenced by the changes in local population growth. Overall, exposure change in the Northwest Pacific is mainly influenced by climate change, followed by population change and joint change.
Sensitivity of Typhoon Forecast to Prescribed Sea Surface Temperature Data
Authors: Park Jinyoung , Cho Woojin , Cha Dong-Hyun , Won Seong-Hee , Lee Jung-Rim
Published: December 2022

This study investigates the impact of the sea surface temperature (SST) on the forecast of two typhoons, which consecutively hit South Korea in 2020. SST data were obtained from the Daily Optimum Interpolation Sea Surface Temperature (OISST) version 2 and HYbrid Coordinate Ocean Model/Navy Coupled Ocean Data Assimilation (HYCOM/NCODA; GLBy0.08/expt_93.0). When verified using in situ observational data, the OISST data did not accurately estimate the changes in SST during each typhoon’s landfall period compared to the HYCOM data since it has a relatively low temporal resolution. To investigate the impact of these two SST data on typhoon forecasts, we conducted sensitivity experiments using the Weather Research and Forecasting (WRF) model. The results showed that simulated typhoon intensities were significantly improved in the simulations with HYCOM data (HY runs), while typhoon track forecast performances were similar in both runs. In addition, the forecast performances of the maximum wind speed at 10 m during the typhoon landfall period were improved in the HY runs. Therefore, this study showed that the overall typhoon intensity and forecast performances during the landfall period could be improved when the higher temporal-resolution SST data were prescribed in the model boundary conditions for a better representation of typhoon-induced SST changes.
The Prebiotic Effect of an Organic Acid Mixture on Faecalibacterium prausnitzii Metabolism and Its Anti-Pathogenic Role against Vibrio parahaemolyticus in Shrimp
Authors: Butucel Eugenia , Balta Igori , McCleery David , Marcu Adela , Stef Ducu , Pet Ioan , Callaway Todd , Stef Lavinia , Corcionivoschi Nicolae
Published: December 2022

Increasing the abundance of probiotic bacteria in the gut requires either direct dietary supplementation or the inclusion of feed additives able to support the growth of beneficial commensal bacteria. In crustaceans, the increased presence of probiotic-like bacteria in the gut, including of Faecalibacterium prausnitzii (F. prausnitzii), will guarantee a positive health status and a gut environment that will ensure enhanced performance. The aim of this study was to investigate if a mixture of organic acids, AuraAqua (Aq) can stimulate the growth and the anti-pathogenic efficacy of F. prausnitzii through a combination of in vitro and ex vivo models. The results showed that 0.5% Aq was able to improve the growth rate of F. prausnitzii in vitro and in an ex vivo shrimp gut model. Moreover, we were able to demonstrate that Aq increases butyrate production and cellulose degradation in culture or in the shrimp gut model. The growth-stimulating effect of Aq also led to an improved and anti-pathogenic effect against Vibrio parahaemolyticus in a co-culture experiment with shrimp gut primary epithelial cells (SGP). In conclusion, our work demonstrates that Aq can stimulate the growth of F. prausnitzii, increase the production of short-chain fatty acid (SCFA) butyrate, improve substrate digestion, and prevent V. parahaemolyticus invasion of SGP cells.
Human Alpha-1 Antitrypsin Attenuates ENaC and MARCKS and Lowers Blood Pressure in Hypertensive Diabetic db/db Mice
Authors: Lugo Carlos I. , Liu Lauren P. , Bala Niharika , Morales Angelica G. , Gholam Mohammed F. , Abchee Julia C. , Elmoujahid Nasseem , Elshikha Ahmed Samir , Avdiaj Rigena , Searcy Louis A. , Denslow Nancy D. , Song Sihong , Alli Abdel A.
Published: December 2022

Hypertension may develop before or after the onset of diabetes and it is known to increase the risk of developing diabetic nephropathy. Alpha-1 antitrypsin (AAT) is a multi-functional protein with beneficial effects in various diseases but its role in reducing blood pressure in the diabetic kidney has not been thoroughly studied. Like blood pressure, epithelial sodium channels (ENaC) and its adaptor protein myristoylated alanine-rich C-kinase substrate (MARCKS) are regulated by circadian rhythms. Our hypothesis is that administration of human AAT (hAAT) reduces blood pressure in hypertensive diabetic mice by attenuating membrane expression of ENaC and its association with the actin cytoskeleton. First, we show hAAT administration results in reduced blood pressure in diabetic db/db mice compared to vehicle treatment in both the inactive and active cycles. Western blotting and immunohistochemistry analyses showed a reduction of ENaC and the actin cytoskeleton protein, MARCKS in the kidneys of diabetic db/db mice treated with hAAT compared to vehicle. hAAT treatment resulted in elevated amounts of extracellular vesicles present in the urine of diabetic db/db mice compared to vehicle treatment both in the inactive and active cycles. Multiple hexosylceramides, among other lipid classes increased in urinary EVs released from hAAT treated hypertensive diabetic mice compared to vehicle treated mice. Taken together, these data suggest hAAT treatment could normalize blood pressure in the diabetic kidney in a mechanism involving attenuation of renal ENaC and MARCKS protein expression and possibly ceramide metabolism to hexosylceramide in kidney cells.
Cancer-Associated Fibroblast Heterogeneity, Activation and Function: Implications for Prostate Cancer
Authors: Owen Jasmine S. , Clayton Aled , Pearson Helen B.
Published: December 2022

The continuous remodeling of the tumor microenvironment (TME) during prostate tumorigenesis is emerging as a critical event that facilitates cancer growth, progression and drug-resistance. Recent advances have identified extensive communication networks that enable tumor–stroma cross-talk, and emphasized the functional importance of diverse, heterogeneous stromal fibroblast populations during malignant growth. Cancer-associated fibroblasts (CAFs) are a vital component of the TME, which mediate key oncogenic events including angiogenesis, immunosuppression, metastatic progression and therapeutic resistance, thus presenting an attractive therapeutic target. Nevertheless, how fibroblast heterogeneity, recruitment, cell-of-origin and differential functions contribute to prostate cancer remains to be fully delineated. Developing our molecular understanding of these processes is fundamental to developing new therapies and biomarkers that can ultimately improve clinical outcomes. In this review, we explore the current challenges surrounding fibroblast identification, discuss new mechanistic insights into fibroblast functions during normal prostate tissue homeostasis and tumorigenesis, and illustrate the diverse nature of fibroblast recruitment and CAF generation. We also highlight the promise of CAF-targeted therapies for the treatment of prostate cancer.
Inactive Proteasomes Routed to Autophagic Turnover Are Confined within the Soluble Fraction of the Cell
Authors: Friedman Keren , Karmon Ofri , Fridman Uri , Goldberg Yair , Pines Ophry , Ben-Aroya Shay
Published: December 2022

Previous studies demonstrated that dysfunctional yeast proteasomes accumulate in the insoluble protein deposit (IPOD), described as the final deposition site for amyloidogenic insoluble proteins and that this compartment also mediates proteasome ubiquitination, a prerequisite for their targeted autophagy (proteaphagy). Here, we examined the solubility state of proteasomes subjected to autophagy as a result of their inactivation, or under nutrient starvation. In both cases, only soluble proteasomes could serve as a substrate to autophagy, suggesting a modified model whereby substrates for proteaphagy are dysfunctional proteasomes in their near-native soluble state, and not as previously believed, those sequestered at the IPOD. Furthermore, the insoluble fraction accumulating in the IPOD represents an alternative pathway, enabling the removal of inactive proteasomes that escaped proteaphagy when the system became saturated. Altogether, we suggest that the relocalization of proteasomes to soluble aggregates represents a general stage of proteasome recycling through autophagy.
Graphitic Carbon Nitride Nanoheterostructures as Novel Platforms for the Electrochemical Sensing of the Chemotherapeutic and Immunomodulator Agent MTX
Authors: Elfarargy Reham G. , Saleh Mahmoud A. , Abodouh Mohamed Mahrous , Hamza Mahmoud A. , Allam Nageh K.
Published: December 2022

We report on the electrochemical determination of one the most effective and widely used chemotherapeutic, anti-inflammatory, and immunomodulator agents, methotrexate (MTX), using low-cost, green, and facile one-pot prepared graphitic carbon nitride (g-CN ) nanosheets. The g-CN nanosheets have been characterized utilizing Fourier transform infrared spectroscopy, X-ray diffraction(XRD), scanning electron microscopy(SEM), and density functional theory (DFT). In comparison to the bare carbon paste electrode (CPE), the g-CN -modified electrode showed a spectacular enhancement in the electrochemical oxidation and detection abilities of MTX. The proposed material exhibits very low limits of detection (12.45 nM) and quantification (41.5 nM), while possessing a wide linear range of 0.22–1.11 μM and 1.11–27.03 μM under optimized conditions at pH 7.0. Due to the ease of preparation of g-CN, it can be adopted for the cost-effective detection of MTX in industrial and clinical analyses.
Palladium Hydroxide (Pearlman’s Catalyst) Doped MXene (Ti3C2Tx) Composite Modified Electrode for Selective Detection of Nicotine in Human Sweat
Authors: Magesh Vasanth , Sundramoorthy Ashok K. , Ganapathy Dhanraj , Atchudan Raji , Arya Sandeep , Alshgari Razan A. , Aljuwayid Ahmed Muteb
Published: December 2022

High concentrations of nicotine (40 to 60 mg) are more dangerous for adults who weigh about 70 kg. Herein, we developed an electrochemical transducer using an MXene (Ti3C2Tx)/palladium hydroxide-supported carbon (Pearlman’s catalyst) composite (MXene/Pd(OH)2/C) for the identification of nicotine levels in human sweat. Firstly, the MXene was doped with Pd(OH)2/C (PHC) by mechanical grinding followed by an ultrasonication process to obtain the MXene/PHC composite. Secondly, XRD, Raman, FE-SEM, EDS and E-mapping analysis were utilized to confirm the successful formation of MXene/PHC composite. Using MXene/PHC composite dispersion, an MXene/PHC composite-modified glassy carbon electrode (MXene/PHC/GCE) was prepared, which showed high sensitivity as well as selectivity towards nicotine (300 µM NIC) oxidation in 0.1 M phosphate buffer (pH = 7.4) by cyclic voltammetry (CV) and amperometry. The MXene/PHC/GCE had reduced the over potential of nicotine oxidation (about 200 mV) and also enhanced the oxidation peak current (8.9 µA) compared to bare/GCE (2.1 µA) and MXene/GCE (5.5 µA). Moreover, the optimized experimental condition was used for the quantification of NIC from 0.25 µM to 37.5 µM. The limit of detection (LOD) and sensitivity were 27 nM and 0.286 µA µM−1 cm2, respectively. The MXene/PHC/GCE was also tested in the presence of Na+, Mg2+, Ca2+, hydrogen peroxide, acetic acid, ascorbic acid, dopamine and glucose. These molecules were not interfered during NIC analysis, which indicated the good selectivity of the MXene/PHC/GCE sensor. In addition, electrochemical determination of NIC was successfully carried out in the human sweat samples collected from a tobacco smoker. The recovery percentage of NIC in the sweat sample was 97%. Finally, we concluded that the MXene/PHC composite-based sensor can be prepared for the accurate determination of NIC with high sensitivity, selectivity and stability in human sweat samples.
Recent Advancements in Augmentation of Solar Water Heaters Using Nanocomposites with PCM: Past, Present, and Future
Authors: Kushwaha Pravesh Kr. , Sharma Neelesh Kr. , Kumar Ashwani , Meena Chandan Swaroop
Published: December 2022

Energy consumption in India is massive, and even the quantity used for household tasks is substantial. The majority of the requirement is satisfied by using fossil fuels, which are the traditional methods. Heating water is the most frequent home application. Accordingly, this article examines studies from the previous ten years. The information in this article demonstrates that using renewable energy is the greatest way to cut back on both the use of fossil fuels and carbon emissions while heating water for residential use. Solar, hydroelectric, wind, and biofuels are the most significant renewable sources for improving building efficiency that can be used for an extended period of time. The solar water heater is a common example of how solar energy is being used in homes more frequently. In order to identify key issues and solutions related to employing solar water heaters as an effective water heating application in both commercial and residential buildings, this article compiles research data from earlier studies (2012–2022). The literature survey was carried out using Scopus, a specialized database. Sixty-six dedicated research publications having search keywords plus recently published articles that matched the inclusion criteria were chosen for this review study. The study’s findings show that there is a greater inclination of researchers towards research and development in the field of domestic solar water heaters. The research publications that are being presented are all from the past 10 years (2012–2022) and stress the use of solar energy in increasing building efficiency. The study highlights how flat plate solar collectors with distilled water as the heat transfer fluid and a phase-changing substance as the thermal energy storage could potentially be enhanced. The thermal conductivity of paraffin wax and distilled water was improved by 75% of the researchers by using 0.05 to 0.5% concentrations of Al and Cu oxide nanoparticles, making it useful in solar water heaters. A total of 78% of researchers are interested in domestic water heating applications since they use a lot of energy in both urban and rural settings.
Comparative Analysis of Buffer and Damper Positions for Increasing the Seismic Performance of Suspension Bridge
Authors: Pan Shengshan , Wang Jichong , Fan Shuli , Tian Kailun , Zhu Weizhi
Published: December 2022

In this research, a finite element model is established to investigate effective seismic control schemes for a self-anchored suspension bridge (SASB) with three towers. Nonlinear dynamic analyses are conducted to evaluate the seismic performance of SASB with different layout schemes of viscous dampers and buffers, which were installed in longitudinal direction and transversal direction, respectively. The responses of the SASB designed with 10 seismic control schemes are compared to ascertain suitable seismic schemes for SASBs. The results show that the number and location of lateral buffers have an important impact on the dynamic characterization of the SASB, especially for the first lateral mode and lateral fundamental frequency. To effectively increase the seismic performance of SASBs with three towers, mounting buffers between the side towers and the main girder of SASBs is an appropriate scheme. The viscous dampers can effectively decrease the dynamic reaction of the towers and longitudinal deformation of the girder under earthquake excitations. The plan involves the installation of dampers between the main concrete stiffening girder and the side towers as the optimal longitudinal seismic scheme for the SASB. The study offers important insights into the seismic design of SASBs with three towers.
Quantifying Energy Reduction and Thermal Comfort for a Residential Building Ventilated with a Window-Windcatcher: A Case Study
Authors: Nouh Ma’bdeh Shouib , Fawwaz Alrebei Odi , Obeidat Laith M. , Al-Radaideh Tamer , Kaouri Katerina , Amhamed Abdulkarem I.
Published: December 2022

Previous studies on window-windcatchers have shown their effectiveness in capturing the prevailing wind and redirecting it into a building, increasing the actual-to-required ventilation ratio by 9%, above what is required by the American Society of Heating, Refrigerating, and Air-Conditioning Engineers (ASHRAE). However, the effect of implementing the proposed system on energy performance, energy costs, and thermal comfort has not been studied. Therefore, here, we investigate and test the implementation of the window-windcatcher on a typical residential building, using a validated DesignBuilder model. Compared to the base case (no window-windcatcher), the total annual energy consumption of the entire building (Etot,b), and consequently the cost, is reduced by approximately 23.3% (i.e., from 18,143 kWh/year to 13,911 kWh/year) when using the window-windcatcher. The total annual reduction in thermal discomfort hours is estimated to be 290 h, which corresponds to an average monthly reduction of approximately 24 h.
Low Frequency of Cancer-Predisposition Gene Mutations in Liver Transplant Candidates with Hepatocellular Carcinoma
Authors: Horackova Klara , Frankova Sona , Zemankova Petra , Nehasil Petr , Cerna Marta , Neroldova Magdalena , Otahalova Barbora , Kral Jan , Hovhannisyan Milena , Stranecky Viktor , Zima Tomas , Safarikova Marketa , Kalousova Marta , Consortium CZECANCA , Novotny Jan , Sperl Jan , Borecka Marianna , Jelinkova Sandra , Vocka Michal , Janatova Marketa , Kleiblova Petra , Kleibl Zdenek , Jirsa Milan , Soukupova Jana
Published: December 2022

Hepatocellular carcinoma (HCC) mainly stems from liver cirrhosis and its genetic predisposition is believed to be rare. However, two recent studies describe pathogenic/likely pathogenic germline variants (PV) in cancer-predisposition genes (CPG). As the risk of de novo tumors might be increased in PV carriers, especially in immunosuppressed patients after a liver transplantation, we analyzed the prevalence of germline CPG variants in HCC patients considered for liver transplantation. Using the panel NGS targeting 226 CPGs, we analyzed germline DNA from 334 Czech HCC patients and 1662 population-matched controls. We identified 48 PVs in 35 genes in 47/334 patients (14.1%). However, only 7/334 (2.1%) patients carried a PV in an established CPG (PMS2, 4×NBN, FH or RET). Only the PV carriers in two MRN complex genes (NBN and RAD50) were significantly more frequent among patients over controls. We found no differences in clinicopathological characteristics between carriers and non-carriers. Our study indicated that the genetic component of HCC is rare. The HCC diagnosis itself does not meet criteria for routine germline CPG genetic testing. However, a low proportion of PV carriers may benefit from a tailored follow-up or targeted therapy and germline testing could be considered in liver transplant recipients.
CD3+ and CD8+ T-Cell-Based Immune Cell Score and PD-(L)1 Expression in Pulmonary Metastases of Microsatellite Stable Colorectal Cancer
Authors: Karjula Topias , Elomaa Hanna , Niskakangas Anne , Mustonen Olli , Puro Iiris , Kuopio Teijo , Ahtiainen Maarit , Mecklin Jukka-Pekka , Seppälä Toni T. , Wirta Erkki-Ville , Sihvo Eero , Väyrynen Juha P. , Yannopoulos Fredrik , Helminen Olli
Published: December 2022

The objective of this study was to evaluate the prognostic value of CD3+ and CD8+ based immune cell score (ICS), programmed death -1 (PD-1) and programmed death ligand -1 (PD-L1) in pulmonary metastases of proficient mismatch repair colorectal cancer (CRC) patients. A total of 101 pulmonary metastases and 62 primary CRC tumours were stained for CD3+, CD8+, PD-1 and PD-L1 expression. The prognostic value of ICS, PD-1/PD-L1 expression in 67 first pulmonary metastases and 61 primary CRC tumour was analysed. Comparative analysis was also performed between primary tumours and pulmonary metastases, as well as between T-cell densities and PD-1/PD-L1 expression. The 5-year overall survival rates of low, intermediate, and high ICS in pulmonary metastases were 10.0%, 25.5% and 47.0% (p = 0.046), respectively. Patients with high vs. low ICS in pulmonary metastases had a significantly better 5-year survival (adjusted HR 0.25, 95% CI 0.09–0.75, p = 0.013). High tumour cell PD-L1 expression in the pulmonary metastases was associated with improved survival (p = 0.024). Primary tumour CD8+ expression was significantly correlated with all T-cell densities in pulmonary metastases. Conclusion: The ICS evaluated from the resected pulmonary metastases of CRC showed significant prognostic value. High PD-L1 expression in pulmonary metastases is associated with favourable prognosis.
Combination of Small Extracellular Vesicle-Derived Annexin A2 Protein and mRNA as a Potential Predictive Biomarker for Chemotherapy Responsiveness in Aggressive Triple-Negative Breast Cancer
Authors: Desai Priyanka P. , Narra Kalyani , James Johanna D. , Jones Harlan P. , Tripathi Amit K. , Vishwanatha Jamboor K.
Published: December 2022

Small extracellular vesicles (sEVs), mainly exosomes, are nanovesicles that shed from the membrane as intraluminal vesicles of the multivesicular bodies, serve as vehicles that carry cargo influential in modulating the tumor microenvironment for the multi-step process of cancer metastasis. Annexin A2 (AnxA2), a calcium(Ca2+)-dependent phospholipid-binding protein, is among sEV cargoes. sEV-derived AnxA2 (sEV-AnxA2) protein is involved in the process of metastasis in triple-negative breast cancer (TNBC). The objective of the current study is to determine whether sEV-AnxA2 protein and/or mRNA could be a useful biomarkers to predict the responsiveness of chemotherapy in TNBC. Removal of Immunoglobulin G (IgG) from the serum as well as using the System Bioscience’s ExoQuick Ultra kit resulted in efficient sEV isolation and detection of sEV-AnxA2 protein and mRNA compared to the ultracentrifugation method. The standardized method was applied to the twenty TNBC patient sera for sEV isolation. High levels of sEV-AnxA2 protein and/or mRNA were associated with stage 3 and above in TNBC. Four patients who responded to neoadjuvant chemotherapy had high expression of AnxA2 protein and/or mRNA in sEVs, while other four who did not respond to chemotherapy had low levels of AnxA2 protein and mRNA in sEVs. Our data suggest that the sEV-AnxA2 protein and mRNA could be a combined predictive biomarker for responsiveness to chemotherapy in aggressive TNBC.
Estimating the Prevalence of a True Oligometastatic Disease
Authors: Szturz Petr , Bonomo Pierluigi , Vermorken Jan B.
Published: December 2022

To delineate a patient group with few distant metastases that could possibly benefit from a curative therapeutic strategy employing a local approach, the term oligometastatic disease (OMD) was introduced into the clinical practice almost 30 years ago [...]
Hepcidin as a Diagnostic Biomarker in Anaemic Lung Cancer Patients
Authors: Wadowska Katarzyna , Błasiak Piotr , Rzechonek Adam , Bil-Lula Iwona , Śliwińska-Mossoń Mariola
Published: December 2022

We aim to describe the characteristics of hepcidin, IL-6, and TNF-α levels in anaemia of lung cancer patients with operative tumour as well as to investigate the potential diagnostic capabilities of hepcidin in combination with IL-6, TNF-α, and acute phase proteins. We present a retrospective study of 112 lung cancer patients (41 women and 71 men) who were surgically treated at the Lower Silesian Centre for Lung Diseases in Wroclaw, Poland. Serum blood samples were collected from all these patients prior to any surgical treatment and used to determine hepcidin, IL-6, TNF-α, SAA1, and CRP concentrations. Patients were also examined with a complete blood count several times during their hospitalization. The female and male groups were divided based on the occurrence of anaemia during their hospitalization. Patients who developed anaemia post-operatively had significantly lower hepcidin concentrations than non-anaemic patients (p = 0.000694 in females with ≥3 complete blood count examinations and p = 0.007905 in males with 2 complete blood count examinations), whereas patients with anaemia since hospital admission had higher hepcidin concentrations. We observed two hepcidin roles related to two cancer anaemia pathogeneses: (1) higher hepcidin concentrations in patients with anaemia since hospital admission (anaemia of inflammation) and (2) lower hepcidin concentrations in patients who developed anaemia after surgery (anaemia of iron deficiency). Our data support the role of hepcidin, IL-6, and TNF-α in cancer-related anaemia and provide diagnostic values for predicting post-operative anaemia in lung cancer patients.
Contemporary Trends in Malignant Peritoneal Mesothelioma: Incidence and Survival in the United States
Authors: Calthorpe Lucia , Romero-Hernandez Fernanda , Miller Phoebe , Conroy Patricia C. , Hirose Kenzo , Kim Alex , Kirkwood Kimberly , Nakakura Eric , Corvera Carlos , Maker Ajay V. , Alseidi Adnan , Adam Mohamed Abdelgadir
Published: December 2022

Background: Malignant peritoneal mesothelioma (MPM) is a rare disease with a historically poor prognosis. Given the emergence of effective therapies, a contemporary analysis of MPM incidence and survival is warranted. Methods: The SEER-18 registry dataset was analyzed (2000–2018). Age-adjusted annual incidence was stratified by sex and histology. Joinpoint regression was used to estimate annual percent change (APC) in incidence. Multivariable cox proportional hazards models were used to investigate survival trends. Results: Of 1689 MPM cases, most were male (55.4%), >50 years (80.0%), and white (75.2%). Age-adjusted incidence of MPM remained stable over time, with an average annual incidence of 1.02 cases/million. Epithelioid histology increased by 240% (APC 2.6; 95% CI: 0.7, 4.5), while incidence of undefined histology decreased significantly (APC −2.1; 95% CI: −3.1, −1.1). Cases treated with cancer-directed surgery increased from 27% to 43%. Overall median age-standardized survival was 11.6 months. Median age-standardized survival was 16.6 months for epithelioid histology but 2.0 months for sarcomatoid histology. Diagnosis in recent years (2015–2018 HR 0.51; 95% CI: 0.38, 0.67) and receipt of cancer-directed surgery (HR 0.84; 95% CI: 0.72, 0.98) were associated with improved survival. Conclusions: Although the overall incidence of MPM remained stable, recognition of epithelioid histology increased. Concurrent with an increase in cancer-directed surgery, MPM survival has improved.
An Insight into Carbon Nanomaterial-Based Photocatalytic Water Splitting for Green Hydrogen Production
Authors: Rasool Muhammad Asghar , Sattar Rabia , Anum Ayesha , Al-Hussain Sami A. , Ahmad Sajjad , Irfan Ali , Zaki Magdi E. A.
Published: December 2022

At present, the energy shortage and environmental pollution are the burning global issues. For centuries, fossil fuels have been used to meet worldwide energy demand. However, thousands of tons of greenhouse gases are released into the atmosphere when fossil fuels are burned, contributing to global warming. Therefore, green energy must replace fossil fuels, and hydrogen is a prime choice. Photocatalytic water splitting (PWS) under solar irradiation could address energy and environmental problems. In the past decade, solar photocatalysts have been used to manufacture sustainable fuels. Scientists are working to synthesize a reliable, affordable, and light-efficient photocatalyst. Developing efficient photocatalysts for water redox reactions in suspension is a key to solar energy conversion. Semiconductor nanoparticles can be used as photocatalysts to accelerate redox reactions to generate chemical fuel or electricity. Carbon materials are substantial photocatalysts for total WS under solar irradiation due to their high activity, high stability, low cost, easy production, and structural diversity. Carbon-based materials such as graphene, graphene oxide, graphitic carbon nitride, fullerenes, carbon nanotubes, and carbon quantum dots can be used as semiconductors, photosensitizers, cocatalysts, and support materials. This review comprehensively explains how carbon-based composite materials function as photocatalytic semiconductors for hydrogen production, the water-splitting mechanism, and the chemistry of redox reactions. Also, how heteroatom doping, defects and surface functionalities, etc., can influence the efficiency of carbon photocatalysts in H2 production. The challenges faced in the PWS process and future prospects are briefly discussed.
Effect of Nitrogen Doping in GO as Support in ZnO/GO-N Compounds and Their Photocatalytic Assessment to Degrade the Lignin Molecule
Authors: Rangel R. , Ramos-Corona A. , Espino J. , Quintana P. , Bartolo-Pérez P. , García R.
Published: December 2022

Control of the recombination process and improvement of transport charge carriers could be achieved in photocatalysts by modifying the catalytic support. In the present study, our goal was to study the effect of nitrogen doping on graphene oxide sheets using doping sources such as urea, thiourea, or ethylenediamine to produce GO-N catalytic supports which were used to form ZnO/GO-N systems. The synthesis of ZnO and GO-N was carried out through a hydrothermal process under microwave heating. The ZnO/GO-N compounds were tested to study the degradation of the lignin molecule under UV irradiation. A set of characterization techniques were used to study the ZnO/GO-N compounds, including XPS analyses which confirmed the N-doping in the samples. The ZnO compound reached 40% of lignin degradation in 70 min, while the ZnO/GO-N compound produced 79% of lignin degradation, also in 70 min evidencing the positive effect of the GO-N support. The best results of degradation were obtained when thiourea was used as the N-doping media.
Correlation Relationship between Phase Inversion of Pickering Emulsions and Biocatalytic Activity of Microbial Transformation of Phytosterols
Authors: Zhao Wenyu , Xie Haisheng , Zhang Xuehong , Wang Zhilong
Published: December 2022

Microbial transformation of hydrophobic phytosterols into the pharmaceutical steroid precursors AD (androst-4-ene-3, 17-dione) and ADD (androst-4-diene-3, 17-dione) in a water–plant oil two-phase system by Mycolicibacterium neoaurum is a paradigm of interfacial biocatalysis in Pickering emulsions stabilized by bacterial cells. In the present work, phase inversion of Pickering emulsions—i.e., Pickering emulsions turning from water-in-oil (W/O) emulsions into oil-in-water (O/W) ones—was observed during microbial transformation in the presence of high concentrations of crystal phytosterols. It was found that there is a correlation relationship between the phase behaviors of Pickering emulsions and the biocatalytic activity of utilizing M. neoaurum as a whole-cell catalyst. Efficient microbial transformation under the high crystal phytosterol loadings was achieved due to the formation of O/W emulsions where interfacial biocatalysis took place. Under the optimal conditions (volume ratio of soybean oil to water: 15:35 mL, phytosterols concentration in the soybean oil: 80 g/L, glucose as co-substrate in the aqueous culture medium: 10 g/L), the concentrations of AD and ADD reached 4.8 g/L based on the whole broth (16 g/L based on the oil phase) after microbial transformation for 9 days.
Arf-like Protein 2 (ARL2) Controls Microtubule Neogenesis during Early Postnatal Photoreceptor Development
Authors: Gerstner Cecilia D. , Reed Michelle , Dahl Tiffanie M. , Ying Guoxin , Frederick Jeanne M. , Baehr Wolfgang
Published: December 2022

Arf-like protein 2 (ARL2) is a ubiquitously expressed small GTPase with multiple functions. In a cell culture, ARL2 participates with tubulin cofactor D (TBCD) in the neogenesis of tubulin αβ-heterodimers, the building blocks of microtubules. To evaluate this function in the retina, we conditionally deleted ARL2 in mouse retina at two distinct stages, either during the embryonic development (retArl2−/−) or after ciliogenesis specifically in rods (rodArl2−/−). retArl2−/− retina sections displayed distorted nuclear layers and a disrupted microtubule cytoskeleton (MTC) as early as postnatal day 6 (P6). Rod and cone outer segments (OS) did not form. By contrast, the rod ARL2 knockouts were stable at postnatal day 35 and revealed normal ERG responses. Cytoplasmic dynein is reduced in retArl2−/− inner segments (IS), suggesting that dynein may be unstable in the absence of a normal MTC. We investigated the microtubular stability in the absence of either ARL2 (retARL2−/−) or DYNC1H1 (retDync1h1−/−), the dynein heavy chain, and found that both the retArl2−/− and retDync1h1−/− retinas exhibited reduced microtubules and nuclear layer distortion. The results suggest that ARL2 and dynein depend on each other to generate a functional MTC during the early photoreceptor development.
The Apelinergic System: Apelin, ELABELA, and APJ Action on Cell Apoptosis: Anti-Apoptotic or Pro-Apoptotic Effect?
Authors: Respekta Natalia , Pich Karolina , Dawid Monika , Mlyczyńska Ewa , Kurowska Patrycja , Rak Agnieszka
Published: December 2022

The apelinergic system comprises two peptide ligands, apelin and ELABELA, and their cognate G-protein-coupled receptor, the apelin receptor APJ. Apelin is a peptide that was isolated from bovine stomach extracts; the distribution of the four main active forms, apelin-36, -17, -13, and pyr-apelin-13 differs between tissues. The mature form of ELABELA-32 can be transformed into forms called ELABELA-11 or -21. The biological function of the apelinergic system is multifaceted, and includes the regulation of angiogenesis, body fluid homeostasis, energy metabolism, and functioning of the cardiovascular, nervous, respiratory, digestive, and reproductive systems. This review summarises the mechanism of the apelinergic system in cell apoptosis. Depending on the cell/tissue, the apelinergic system modulates cell apoptosis by activating various signalling pathways, including phosphoinositide 3-kinase (PI3K), extracellular signal-regulated protein kinase (ERK1/2), protein kinase B (AKT), 5’AMP-activated protein kinase(AMPK), and protein kinase A (PKA). Apoptosis is critically important during various developmental processes, and any dysfunction leads to pathological conditions such as cancer, autoimmune diseases, and developmental defects. The purpose of this review is to present data that suggest a significant role of the apelinergic system as a potential agent in various therapies.
Influence of Anodizing by Electro-Chemical Oxidation on Fatigue and Wear Resistance of the EV31A-T6 Cast Magnesium Alloy
Authors: Di Egidio Gianluca , Tonelli Lavinia , Morri Alessandro , Boromei Iuri , Shashkov Pavel , Martini Carla
Published: December 2022

In the last decades, several anodizing processes for Mg alloys have been proposed to achieve a good wear and corrosion resistance combination. In particular, Electro-Chemical Oxidation (ECO) showed an improved dense and compact anodized layer compared to other anodizing processes carried out above the dielectric breakdown voltage, such as Plasma Electrolytic Oxidation (PEO). However, the influence of the ECO treatment on the tribological behavior and cyclic mechanical performance of Mg alloys has not been investigated yet. This paper reports on the influence of ECO on dry sliding behavior (vs. 100Cr6 bearing steel (block-on-ring contact geometry)) and rotating bending fatigue performance of the rare earth (RE)-containing Mg alloy EV31A-T6, comparing it with both untreated EV31A-T6 and PEO-treated EV31A-T6, used as benchmarks. The ECO-treated alloy showed improved tribological behavior (critical load for coating failure one order of magnitude higher and coefficient of friction 40% lower than for PEO) and fatigue strength (no decrease for ECO-treated samples compared to the untreated alloy, while PEO-treated samples induced a 15% decrease) due to the increased compactness and lower defectivity of the anodized layer, induced by the minimization of destructive arc discharges during coating growth. In addition, the ECO treatment significantly improved wear resistance compared to the untreated alloy, avoiding, at the same time, the decrease in fatigue strength, which typically occurs after PEO. Therefore, the ECO process can be applied to improve wear resistance without decreasing the fatigue strength of high-performance components.
Special Issue: Recent Trends in Wear and Erosion Resistance of Alloys
Authors: Zaki Elsayed Gamal , Selim Mohamed S. , Hao Zhifeng , ElSaeed S. M. , EL-Saeed Ashraf M.
Published: December 2022

The development of novel alloys with enhanced wear and erosion resistance has attracted a lot of interest [...]