bluuebunny/crossref_metadata_2025 huggingface dataset:
Special Issue on Set Valued Analysis 2021
Authors: Croitoru Anca , Mesiar Radko , Sambucini Anna Rita , Satco Bianca
Published: August 2022

Set Valued Analysis plays an important role in the study of statistics, biology, economics, social sciences, optimal control, differential inclusions, image reconstruction and fixed point theory [...]
High-Dimensional Statistics: Non-Parametric Generalized Functional Partially Linear Single-Index Model
Authors: Alahiane Mohamed , Ouassou Idir , Rachdi Mustapha , Vieu Philippe
Published: August 2022

We study the non-parametric estimation of partially linear generalized single-index functional models, where the systematic component of the model has a flexible functional semi-parametric form with a general link function. We suggest an efficient and practical approach to estimate (I) the single-index link function, (II) the single-index coefficients as well as (III) the non-parametric functional component of the model. The estimation procedure is developed by applying quasi-likelihood, polynomial splines and kernel smoothings. We then derive the asymptotic properties, with rates, of the estimators of each component of the model. Their asymptotic normality is also established. By making use of the splines approximation and the Fisher scoring algorithm, we show that our approach has numerical advantages in terms of the practical efficiency and the computational stability. A computational study on data is provided to illustrate the good practical behavior of our methodology.
Thermodynamic Definition of Time: Considerations on the EPR Paradox
Authors: Lucia Umberto , Grisolia Giulia
Published: August 2022

Causality is the relationship between causes and effects. Following Relativity, any cause of an event must always be in the past light cone of the event itself, but causes and effects must always be related to some interactions. In this paper, causality is developed as a consequence of the analysis of the Einstein, Podolsky, and Rosen paradox. Causality is interpreted as the result of time generation, due to irreversible interactions of real systems among them. Time results as a consequence of irreversibility; so, any state function of a system in its space cone, when affected by an interaction with an observer, moves into a light cone or within it, with the consequence that any cause must precede its effect in a common light cone.
Fabrication of a Novel (PVDF/MWCNT/Polypyrrole) Antifouling High Flux Ultrafiltration Membrane for Crude Oil Wastewater Treatment
Authors: Hudaib Banan , Abu-Zurayk Rund , Waleed Haneen , Ibrahim Abed Alqader
Published: August 2022

The present work deals with the fabrication of novel poly(vinylidene fluoride) (PVDF)/Multi-wall Carbon Nanotubes (MWCNT)/Polypyrrole (PPy) ultrafiltration membrane by phase inversion technique for the removal of crude oil from refinery wastewater. In situ polymerization of pyrrole with different concentrations of MWCNT ranging from 0.025 wt.% to 0.3 wt.% in PVDF prepared solutions. Measurement of permeability, porosity, contact angle, tensile strength, zeta potential, rejection studies and morphological characterization by scanning electron microscopy (SEM) were conducted. The results showed that membrane with (0.05% MWCNT) concentration had the highest permeability flux (850 LMH/bar), about 17 folds improvement of permeability compared to pristine PVDF membrane. Moreover, membrane rejection of crude oil reached about 99.9%. The excellent performance of this nanocomposite membrane suggests that novel PVDF modification with polypyrrole had a considerable effect on permeability with high potential for use in the treatment of oily wastewater in the refinery industry.
Molecular Dynamics Simulation of Transport Mechanism of Graphene Quantum Dots through Different Cell Membranes
Authors: Zhang Pengzhen , Jiao Fangfang , Wu Lingxiao , Kong Zhe , Hu Wei , Liang Lijun , Zhang Yongjun
Published: August 2022

Exploring the mechanisms underlying the permeation of graphene quantum dots (GQDs) through different cell membranes is key for the practical application of GQDs in medicine. Here, the permeation process of GQDs through different lipid membranes was evaluated using molecular dynamics (MD) simulations. Our results showed that GQDs can easily permeate into 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) lipid membranes with low phospholipid molecule densities but cannot permeate into 1-palmitoyl-2-oleoyl phosphatidylethanolamine (POPE) lipid membranes with high phospholipid densities. Free energy calculation showed that a high-energy barrier exists on the surface of the POPE lipid membrane, which prevents GQDs from entering the cell membrane interior. Further analysis of the POPE membrane structure showed that sparsely arranged phospholipid molecules of the low-density lipid membrane facilitated the entry of GQDs into the interior of the membrane, compared to compactly arranged molecules in the high-density lipid membrane. Our simulation study provides new insights into the transmembrane transport of GQDs.
Influence of Rare Earth Samarium/Ytterbium Salt on Electrochemical Corrosion Behavior of Aluminum-Based Anode for Batteries
Authors: Shi Bangan , Zhang Yunxu , Wang Ranshu , Wang Yong , Li Cunyong
Published: August 2022

In this work, effects and mechanism analysis of samarium acetate and ytterbium acetate on enhancing the electrochemical corrosion performance of aluminum-based anode for aluminum-air batteries in 3.5 wt.% NaCl are studied by methods such as weight loss tests, electrochemical measurements, anode galvanostatic discharge tests and microscopic morphology analysis. The results show that samarium acetate and ytterbium acetate are ideal electrolyte additives, and exhibit obvious inhibitory effects on the self-corrosion of 7075 aluminum alloy. The optimal concentration is 200 mg/L. Moreover, corrosion inhibitors mainly reduce the self-corrosion speed of aluminum by suppressing the microcathodic reaction, thereby promoting the improvement of the discharge performance of aluminum-air batteries. Simultaneously, it is found that after mixing samarium acetate and ytterbium acetate in different proportions, the two rare earth salts have a mutual adjustment effect. By adding different rare earth salt components, the battery capacity densities of the anode are improved by 9.6% to 16.3%. Finally, a possible model is presented to illustrate the impact mechanism of different additives on the self-corrosion process and discharge performance of aluminum-air batteries.
Study of Material Properties and Creep Behavior of a Large Block of AISI 316L Steel Produced by SLM Technology
Authors: Hermanová Šárka , Kuboň Zdeněk , Čížek Petr , Kosňovská Jana , Rožnovská Gabriela , Dorazil Ondřej , Cieslarová Marcela
Published: August 2022

The additive manufacturing (3D printing) of metallic materials is a relatively new technology and its use is quickly increasing. Although it is of interest to many researchers, there are still areas which are not fully explored. One of those areas is the behavior of large components and/or semi-products processed by 3D printing. This work is focused on the study of material properties of additive manufactured large block made of AISI 316L steel in two heat treatment conditions (as-printed and solution annealed) and their comparison with the properties of hot-rolled plate performed by tensile tests, Charpy V-notch tests, small punch tests and stress rupture tests. Mechanical tests were complemented by microstructural investigation and the fractographic analysis of fracture surfaces. We found out that mechanical and long-term properties of large 3D printed blocks of this steel are excellent and comparable with other published results obtained on small-sized and intentionally produced test pieces. The observed lower ductility is the result of printing imperfections in microstructure. The results of small punch tests confirmed the possibility of exploiting the existing database and using the correlation between small punch tests and tensile tests results even for 3D-printed AISI 316L steel.
Ageing Susceptibility of Continuously Annealed Low-Carbon-Steel Strips
Authors: Bi Yunjie , Ge Rui , Yan Ming , Xiong Xiaojing
Published: August 2022

The continuously annealed low-carbon-steel strips from industrial production showed an average aging index of about 30 N/mm². This increase in yield strength caused by the continuous-annealing process was strongly dependent on the soaking temperature and the soaking time. Whilst the optimization of the overaging parameters and the use of a higher annealing temperature would reduce the ageing susceptibility of low-carbon-steel strips, it was considered unlikely that its magnitude could be reduced to a comparable level to that of the batch-annealed one. Within the limit of low-carbon-strip specifications, there was very little effect of the C content on the ageing. The aging index of the temper-rolled materials decreased with the increase in the temper-rolling reductions, and a maximum increase in yield strength was achieved at a temper-rolling reduction of about 1%. In this work, the effect of C content, Al content, continuous-annealing cycles, and temper-rolling reduction on the ageing susceptibility of the continuously annealed low-carbon-steel strips was examined. SEM/EBSD technique was developed to characterize the temper-rolling strain bands and to assess the effect of temper-rolling reduction on the yield strength of the low-carbon-steel strips.
Neural Network Prediction Model for Sinter Mixture Water Content Based on KPCA-GA Optimization
Authors: Ren Yuqian , Huang Chuanqi , Jiang Yushan , Wu Zhaoxia
Published: August 2022

The design and optimization of a sinter mixture moisture controlling system usually require complex process mechanisms and time-consuming field experimental simulations. Based on BP neural networks, a new KPCA-GA optimization method is proposed to predict the mixture moisture content sequential values with time more accurately so as to derive the optimal water addition to meet industrial requirements. Firstly, the normalized input variables affecting the output were dimensionalized using kernel principal component analysis (KPCA), and the contribution rates of the factors affecting the water content were analyzed. Then, a BP neural network model was established. In order to get rid of the randomness of the initial threshold and weights on the prediction accuracy of the model, a genetic algorithm is proposed to preferentially find the optimal initial threshold and weights for the model. Then, statistical indicators, such as the root mean square error, were used to evaluate the fit and prediction accuracy of the training and test data sets, respectively. The available experimental data show that the KPCA-GA model has high fitting and prediction accuracy, and the method has significant advantages over traditional neural network modeling methods when dealing with data sets with complex nonlinear characteristics, such as those from the sintering process.
Improving Mechanical and Corrosion Behavior of 5052 Aluminum Alloy Processed by Cyclic Extrusion Compression
Authors: Wu Jianxin , Djavanroodi Faramarz , Shamsborhan Mahmoud , Attarilar Shokouh , Ebrahimi Mahmoud
Published: August 2022

Background The severe plastic deformation approach and its well-known cyclic extrusion compression (CEC) method have been established as a powerful tool for fabricating bulk ultrafine-grained metals and alloys with improved properties. Objective This study focused on the microstructure evolution, hardness behavior, and corrosion properties of the CEC-processed Al5052 up to four passes compared to the initial annealed state. Methods The initial and CEC-processed Al5052 samples at different pass numbers were examined experimentally by EBSD analyses, hardness measurements, and corrosion resistance. Results Substantial grain refinement was attained from ~23 μm for the annealed sample to ~0.8 μm in the four passes sample. In addition, the hardness values considerably increased up to 75.7% after four passes from the initial value of 80 HV. In addition, the increment of pass numbers led to a more uniform dispersion of hardness values. Furthermore, the production of more stable protective oxide layers on the UFG structure of the CEC-processed sample led to the improvement in electrochemical response with a corrosion rate reduction from 1.49 to 1.02 mpy, respectively, in the annealed and final pass CEC-processed samples. In fact, the annealed sample manifested more large-sized and deeper pits than the CECed samples due to the increment of potential values and electrochemical attack of chlorine ions that finally deteriorates the corrosion performance. Conclusions CEC is an efficient method to improve the mechanical properties of materials due to substantial microstructural changes along with enhancement of electrochemical behavior because of the presence of small-sized and shallow pits.
The Effect of Mn on the Mechanical Properties and In Vitro Behavior of Biodegradable Zn-2%Fe Alloy
Authors: Ben Tzion-Mottye Lital , Ron Tomer , Eliezer Dan , Aghion Eli
Published: August 2022

The attractiveness of Zn-based alloys as structural materials for biodegradable implants mainly relates to their excellent biocompatibility, critical physiological roles in the human body and excellent antibacterial properties. Furthermore, in in vivo conditions, they do not tend to produce hydrogen gas (as occurs in the case of Mg-based alloys) or voluminous oxide (as occurs in Fe-based alloys). However, the main disadvantages of Zn-based alloys are their reduced mechanical properties and their tendency to provoke undesirable fibrous encapsulation due to their relatively high standard reduction potential. The issue of fibrous encapsulation was previously addressed by the authors via the development of the Zn-2%Fe alloy that was selected as the base alloy for this study. This development assumed that the addition of Fe to pure Zn can create a microgalvanic effect between the Delta phase (Zn11Fe) and the Zn-matrix that significantly increases the biodegradation rate of the alloy. The aim of the present study is to examine the effect of up to 0.8% Mn on the mechanical properties of biodegradable Zn-2%Fe alloy and to evaluate the corrosion behavior and cytotoxicity performance in in vitro conditions. The selection of Mn as an alloying element is related to its vital role in the synthesis of proteins and the activation of enzyme systems, as well as the fact that Mn is not considered to be a toxic element. Microstructure characterization was carried out by optical microscopy and scanning electron microscopy (SEM), while phase analysis was obtained by X-ray diffraction (XRD). Mechanical properties were examined in terms of hardness and tensile strength, while corrosion performance and electrochemical behavior were assessed by immersion tests, open circuit potential examination, potentiodynamic polarization analysis and impedance spectroscopy. All the in vitro corrosion testing was performed in a simulated physiological environment in the form of a phosphate-buffered saline (PBS) solution. The cytotoxicity performance was evaluated by indirect cell viability analysis, carried out according to the ISO 10993-5/12 standard using Mus musculus 4T1 cells. The obtained results clearly demonstrate the strengthening effect of the biodegradable Zn-2%Fe alloy due to Mn addition. The effect of Mn on in vitro corrosion degradation was insignificant, while in parallel Mn had a favorable effect on indirect cell viability.
Mechanical Activation-Assisted Solid-State Aluminothermic Reduction of CuO Powders for In-Situ Copper Matrix Composite Fabrication
Authors: Arasteh Sahand , Masoudi Afshin , Abbasi Alireza , Lotfian Saeid
Published: August 2022

In this study, combustion synthesis involving mechanical milling and subsequent sintering process was utilised to fabricate Cu/AlxCuy/Al2O3 in-situ composite through the aluminothermic reduction of CuO powders. First, CuO and Al powders were mixed, and ball milled for 30–150 min to facilitate self-propagating high-temperature synthesis (SHS). Then, mechanically activated Al-CuO powders were mixed with elemental Cu powders and experienced subsequent cold compaction and sintering processes. The reactions during synthesis were studied utilising differential thermal analysis (DTA), X-ray diffraction (XRD), and scanning electron microscopy (SEM). Densification and hardness of green and sintered bodies were also obtained. The results indicated that despite the negative free energy of the aluminothermic reaction, an initial activation energy supply is required, and mixed Al-CuO powders did not show significant progress in the combustion synthesis method. The aluminothermic reaction became probable whenever the activation energy was entirely provided by high-energy ball milling or by the sintering of ball-milled Al-CuO mixed powders. DTA results showed that the aluminothermic reaction temperature of Al-CuO decreased with milling times, whereas after 150 min of ball milling, the reaction was completed. XRD patterns revealed that the formation of Al2Cu and Al2O3 reinforcing phases resulted from CuO reduction with Al. Al4Cu9, Cu solid solution, and Al oxide phases were observed in sintered samples. The relative density of the samples was reduced compared to the green compacted parts due to the nature of the Cu-Al alloy and the occurrence of the swelling phenomenon. The hardness results indicated that in-situ formation of reinforcing phases in samples that experienced thermally assisted thermite reaction yielded superior hardness.
Crankshaft HCF Research Based on the Simulation of Electromagnetic Induction Quenching Approach and a New Fatigue Damage Model
Authors: Sun Songsong , Liu Weiqiang , Zhang Xingzhe , Wan Maosong
Published: August 2022

In recent decades, the electromagnetic induction quenching approach has been widely applied in the surface treatment process of steel engine parts such as crankshafts. In this paper, the strengthening effect of this approach was selected to be the object of study. First, the multi-physics coupling phenomenon was established by a 3D finite element simulation approach. Then, the fatigue property of the crankshaft was predicted based on the combination of the residual stress field obtained in the previous step and a chosen multi-axial fatigue damage model. Finally, a corresponding experiment verification was carried out to check the accuracy of the prediction. The results showed that the method proposed by this paper can provide high enough accuracy in predicting the fatigue property of two types of commonly used steel crankshafts.
Wnt Inhibitors and Bone Mineral Density in Patients with Graves’ Disease Treated with Antithyroid Drugs: A Preliminary Prospective Study
Authors: Mudri Dunja , Kizivat Tomislav , Mihaljević Ivica , Bilić Ćurčić Ines
Published: August 2022

This study aimed to investigate the association of Wnt inhibitors with thyroid hormones, bone turnover markers, and bone mineral density (BMD) in patients with newly diagnosed Graves’ disease (GD) at the beginning of the antithyroid treatment and after a follow-up period of one year. The study included 37 patients with newly diagnosed GD who were treated with antithyroid drugs (ATD). At baseline and after one year, thyroid hormones and thyroid-stimulating hormone (TSH), serum concentrations of sclerostin, and Dickkopf-1 (DKK1) were measured by an enzyme-linked immunosorbent assay (ELISA). In addition, BMD was measured by dual-energy X-ray absorptiometry (DXA), and markers of bone turnover including osteocalcin (OC), beta-cross laps (β-CTX), and deoxypyridinoline (DPD) were determined. After one year of ATD therapy sclerostin levels were significantly decreased (p < 0.001), whereas DKK1 levels were significantly increased (p = 0.01). In addition, BMD of the lumbar spine, total hip, and femoral neck was significantly improved (p < 0.001), accompanied by an increase in OC, β-CTX, and DPD concentrations (p < 0.001). At baseline, sclerostin levels were positively associated with free triiodothyronine (FT3). Following ATD therapy, a positive correlation was observed between FT3 and DKK1 (p = 0.003), whereas a negative correlation was found between TSH and DKK1 (p = 0.04). Correlation analysis demonstrated no association of the sclerostin and DKK1 with other bone remodeling biomarkers OC, β-CTX, or DPD. Also, no significant correlation between sclerostin or DKK1 and T-score or BMD of the lumbar spine, hip, and femoral neck was observed at both time points. Conclusion: Observed differences in sclerostin and DKK1 serum following GD treatment indicate involvement of Wnt inhibitors in the etiopathogenesis of bone loss associated with hyperthyroidism. Furthermore, both sclerostin and DKK1 are involved in the reversal of changes in bone metabolism following ATD therapy, thus presenting potentially valuable bone remodeling markers worth further investigation.
Human Motion Pattern Recognition and Feature Extraction: An Approach Using Multi-Information Fusion
Authors: Li Xin , Liu Jinkang , Huang Yijing , Wang Donghao , Miao Yang
Published: August 2022

An exoskeleton is a kind of intelligent wearable device with bioelectronics and biomechanics. To realize its effective assistance to the human body, an exoskeleton needs to recognize the real time movement pattern of the human body in order to make corresponding movements at the right time. However, it is of great difficulty for an exoskeleton to fully identify human motion patterns, which are mainly manifested as incomplete acquisition of lower limb motion information, poor feature extraction ability, and complicated steps. Aiming at the above consideration, the motion mechanisms of human lower limbs have been analyzed in this paper, and a set of wearable bioelectronics devices are introduced based on an electromyography (EMG) sensor and inertial measurement unit (IMU), which help to obtain biological and kinematic information of the lower limb. Then, the Dual Stream convolutional neural network (CNN)-ReliefF was presented to extract features from the fusion sensors’ data, which were input into four different classifiers to obtain the recognition accuracy of human motion patterns. Compared with a single sensor (EMG or IMU) and single stream CNN or manual designed feature extraction methods, the feature extraction based on Dual Stream CNN-ReliefF shows better performance in terms of visualization performance and recognition accuracy. This method was used to extract features from EMG and IMU data of six subjects and input these features into four different classifiers. The motion pattern recognition accuracy of each subject under the four classifiers is above 97%, with the highest average recognition accuracy reaching 99.12%. It can be concluded that the wearable bioelectronics device and Dual Stream CNN-ReliefF feature extraction method proposed in this paper enhanced an exoskeleton’s ability to capture human movement patterns, thus providing optimal assistance to the human body at the appropriate time. Therefore, it can provide a novel approach for improving the human-machine interaction of exoskeletons.
Microrobots in Micromachines
Authors: Fortuna Luigi , Buscarino Arturo
Published: August 2022

Robotics and micromachines are challenging topics in engineering [...]
A Wideband High-Gain Microstrip Array Antenna Integrated with Frequency-Selective Surface for Sub-6 GHz 5G Applications
Authors: Alwareth Husam , Ibrahim Imran Mohd , Zakaria Zahriladha , Al-Gburi Ahmed Jamal Abdullah , Ahmed Sharif , Nasser Zayed A.
Published: August 2022

This paper presents a wideband and high-gain rectangular microstrip array antenna with a new frequency-selective surface (FSS) designed as a reflector for the sub-6 5G applications. The proposed antenna is designed to meet the US Federal Communications Commission (FCC) standard for 5G in the mid-band (3.5–5 GHz) applications. The designed antenna configuration consists of 1 × 4 rectangular microstrip array antenna with an FSS reflector to produce a semi-stable high radiation gain. The modeled FSS delivered a wide stopband transmission coefficient from 3.3 to 5.6 GHz and promised a linearly declining phase over the mid-band frequencies. An equivalent circuit (EC) model is additionally performed to verify the transmission coefficient of the proposed FSS structure for wideband signal propagation. A low-cost FR-4 substrate material was used to fabricate the antenna prototype. The proposed wideband array antenna with an FSS reflector attained a bandwidth of 2.3 GHz within the operating frequency range of 3.5–5.8 GHz, with a fractional bandwidth of 51.12%. A high gain of 12.4 dBi was obtained at 4.1 GHz with an improvement of 4.4 dBi compared to the antenna alone. The gain variation was only 1.0 dBi during the entire mid-band. The total dimension of the fabricated antenna prototype is 10.32 λo × 4.25 λo ×1.295 λo at a resonance frequency of 4.5 GHz. These results make the presented antenna appropriate for 5G sub-6 GHz applications.
Enhancing Mixing Performance in a Rotating Disk Mixing Chamber: A Quantitative Investigation of the Effect of Euler and Coriolis Forces
Authors: Lee Jihyeong , Lee Saebom , Lee Minki , Prakash Ritesh , Kim Hyejeong , Cho Gyoujin , Lee Jinkee
Published: August 2022

Lab-on-a-CD (LOCD) is gaining importance as a diagnostic platform due to being low-cost, easy-to-use, and portable. During LOCD usage, mixing and reaction are two processes that play an essential role in biochemical applications such as point-of-care diagnosis. In this paper, we numerically and experimentally investigate the effects of the Coriolis and Euler forces in the mixing chamber during the acceleration and deceleration of a rotating disk. The mixing performance is investigated under various conditions that have not been reported, such as rotational condition, chamber aspect ratio at a constant volume, and obstacle arrangement in the chamber. During disk acceleration and deceleration, the Euler force difference in the radial direction causes rotating flows, while the Coriolis force induces perpendicular vortices. Increasing the maximum rotational velocity improves the maximum rotational displacement, resulting in better mixing performance. A longer rotational period increases the interfacial area between solutions and enhances mixing. Mixing performance also improves when there is a substantial difference between Euler forces at the inner and outer radii. Furthermore, adding obstacles in the angular direction also passively promotes or inhibits mixing by configuration. This quantitative investigation provides valuable information for designing and developing high throughput and multiplexed point-of-care LOCDs.
Ultra-Thin and Lithography-Free Transmissive Color Filter Based on Doped Indium Gallium Zinc Oxide with High Performance
Authors: Fan Xiangrui , Wang Shengyao , Xu Dongdong , Zheng Gaige
Published: August 2022

A kind of ultra-thin transmissive color filter based on a metal-semiconductor-metal (MSM) structure is proposed. The displayed color can cover the entire visible range and switches after H2 treatment. An indium gallium zinc oxide (IGZO) semiconductor was employed, as the concentration of charge carriers can be controlled to adjust the refractive index and achieve certain colors. The color modulation in the designed structure was verified using the rigorous coupled wave analysis (RCWA) method. The angular independence of the relative transmission could reach up to 60°, and polarization-insensitive performance could also be maintained. Numerical results demonstrated that the thickness of IGZO was the key parameter to concentrate the incident light. The overall structure is planar and lithography-free and can be produced with simple preparation steps. The obtained results can also be extended to other similar resonators where a proper cavity allows dynamical functionality.
Application of High Potential Electrophoretic Particles Modified with High Ionization Mono Ionic Liquid for Electrophoretic Displays
Authors: Zhang Zhi , Wang Yao , Chen Qun , Gao Qingguo , Liu Liming , Yang Jianjun , Pan Xinjian , Miao Yu , Chi Feng
Published: August 2022

The electrophoretic display (EPD) has attracted widespread attention due to its great visual perception, energy-saving, portability, and bistability. However, the EPD still has many problems in response time, colorization, etc., which limits its practical application. In this paper, novel blue electrophoretic particles were prepared with copper (II) phthalocyanine and high ionization 1-butyl-1-methyl piperidinium bromide mono ionic liquid. It was shown that electrophoretic particles dispersed in a non-polar tetrachloroethylene medium had high Zeta potential and electrophoretic mobility. At the same time, electrophoretic particles showed better dispersion stability. Finally, the prepared blue electrophoretic particles and white titanium dioxide particles were compounded to prepare blue and white dual-color electrophoretic dispersion. An EPD cell was made to test its performance. The results showed that the prepared blue and white dual-color electrophoretic dispersion could realize a reversible response. Piperidine mono ionic liquid increased the surface potential of copper (II) phthalocyanine from +30.50 mV to +60.27 mV, enhancing it by 97.61%. Therefore, we believed that modifying particles with high ionization mono ionic liquid had great applicability to the modification of electrophoretic particles, and blue particles prepared with piperidine mono ionic liquid as a charge control agent (CCA) were excellent candidates for EPDs.
ArsR Family Regulator MSMEG_6762 Mediates the Programmed Cell Death by Regulating the Expression of HNH Nuclease in Mycobacteria
Authors: Duan Xiangke , Huang Xue , Xu Junqi , Li Xue , Niu Jingjing , Du Xiaoli , Wang Xiaoyu , Li Jiang , Kelly Michael , Guo Jiaohan , Zhang Ke , Huang Yu , Kan Biao , Xie Jianping
Published: August 2022

Programmed cell death (PCD) is the result of an intracellular program and is accomplished by a regulated process in both prokaryotic and eukaryotic organisms. Here, we report a programed cell death process in Mycobacterium smegmatis, an Actinobacteria species which involves a transcription factor and a DNase of the HNH family. We found that over-expression of an ArsR family member of the transcription factor, MSMEG_6762, leads to cell death. Transcriptome analysis revealed an increase in the genes’ transcripts involved in DNA repair and homologous recombination, and in three members of HNH family DNases. Knockout of one of the DNase genes, MSMEG_1275, alleviated cell death and its over-expression of programmed cell death. Purified MSMEG_1275 cleaved the M. smegmatis DNA at multiple sites. Overall, our results indicate that the MSMEG_6762 affects cell death and is mediated, at least partially, by activation of the HNH nuclease expression under a stress condition.
Phytoplankton Sources and Sinks of Dimethylsulphoniopropionate (DMSP) in Temperate Coastal Waters of Australia
Authors: Fernandez Eva , Seymour Justin R. , Petrou Katherina
Published: August 2022

The ecologically important organic sulfur compound, dimethylsulfoniopropionate (DMSP), is ubiquitous in marine environments. Produced by some species of phytoplankton and bacteria, it plays a key role in cellular responses to environmental change. Recently, uptake of DMSP by non-DMSP-producing phytoplankton species has been demonstrated, highlighting knowledge gaps concerning DMSP distribution through the marine microbial food web. In this study, we traced the uptake and distribution of DMSP through a natural marine microbial community collected from off the eastern coastline Australia. We found a diverse phytoplankton community representing six major taxonomic groups and conducted DMSP-enrichment experiments both on the whole community, and the community separated into large (≥8.0 µm), medium (3.0–8.0 µm), and small (0.2–3.0 µm) size fractions. Our results revealed active uptake of DMSP in all three size fractions of the community, with the largest fraction (>8 µm) forming the major DMSP sink, where enrichment resulted in an increase of DMSPp by 144%. We observed evidence for DMSP catabolism in all size fractions with DMSP enrichment, highlighting loss from the system via MeSH or DMS production. Based on taxonomic diversity, we postulate the sources of DMSP were the dinoflagellates, Phaeocystis sp., and Trichodesmium sp., which were present in a relatively high abundance, and the sinks for DMSP were the diatoms and picoeucaryotes in this temperate community. These findings corroborate the role of hitherto disregarded phytoplankton taxa as potentially important players in the cycling of DMSP in coastal waters of Australia and emphasize the need to better understand the fate of accumulated DMSP and its significance in cellular metabolism of non-DMSP producers.
Biochemical Diversity, Pathogenicity and Phylogenetic Analysis of Pseudomonas viridiflava from Bean and Weeds in Northern Spain
Authors: Fernández-Sanz Ana M. , Rodicio M. Rosario , González Ana J.
Published: August 2022

Pseudomonas viridiflava was originally reported as a bean pathogen, and subsequently as a wide-host range pathogen affecting numerous plants species. In addition, several authors have reported the epiphytic presence of this bacterium in “non-host plants”, which may act as reservoir of P. viridiflava and source of inoculum for crops. A new biotype of this bacterium, showing an atypical LOPAT profile, was found in Asturias, a Northern region of Spain, causing significant damage in beans, kiwifruit, lettuce, and Hebe. In order to investigate the involvement of weeds in bean disease, samples were collected from beans and weeds growing in the same fields. A total of 48 isolates of P. viridiflava were obtained, 39 from weeds and 9 from beans. 48% and 52% of them showed typical (L− O− P+ A− T+) and atypical (L+ O− P v A− T+) LOPAT profiles, and they displayed high biochemical diversity. Regarding virulence factors, the T-PAI and S-PAI pathogenicity islands were found in 29% and 70.8% of the isolates, 81.2% displayed pectinolytic activity on potato slices, and 59% of the weed isolates produced symptoms after inoculation on bean pods. A phylogenetic tree based on concatenated rpoD, gyrB, and gltA sequences separated the strains carrying S-PAI and T-PAI into different clusters, both containing isolates from beans and weeds, and pathogenic as well as non-pathogenic strains. Closely related strains were found in the two hosts, and more than half of the weed isolates proved to be pathogenic in beans. This is consistent with the role of weeds as a reservoir and source of inoculum for bean infection. Detection of P. viridiflava in weeds throughout the year further supports these roles.
Longitudinal Study of Selected Bacterial Zoonoses in Small Ruminants in Tana River County, Kenya
Authors: Wainaina Martin , Lindahl Johanna F. , Dohoo Ian , Mayer-Scholl Anne , Roesel Kristina , Mbotha Deborah , Roesler Uwe , Grace Delia , Bett Bernard , Al Dahouk Sascha
Published: August 2022

Brucellosis, Q fever, and leptospirosis are priority zoonoses worldwide, yet their epidemiology is understudied, and studies investigating multiple pathogens are scarce. Therefore, we selected 316 small ruminants in irrigated, pastoral, and riverine settings in Tana River County and conducted repeated sampling for animals that were initially seronegative between September 2014 and June 2015. We carried out serological and polymerase chain reaction tests and determined risk factors for exposure. The survey-weighted serological incidence rates were 1.8 (95% confidence intervals [CI]: 1.3–2.5) and 1.3 (95% CI: 0.7–2.3) cases per 100 animal-months at risk for Leptospira spp. and C. burnetii, respectively. We observed no seroconversions for Brucella spp. Animals from the irrigated setting had 6.83 (95% CI: 2.58–18.06, p-value = 0.01) higher odds of seropositivity to C. burnetii than those from riverine settings. Considerable co-exposure of animals to more than one zoonosis was also observed, with animals exposed to one zoonosis generally having 2.5 times higher odds of exposure to a second zoonosis. The higher incidence of C. burnetii and Leptospira spp. infections, which are understudied zoonoses in Kenya compared to Brucella spp., demonstrate the need for systematic prioritization of animal diseases to enable the appropriate allocation of resources.
Proximate Drivers of Population-Level Lizard Gut Microbial Diversity: Impacts of Diet, Insularity, and Local Environment
Authors: Lemieux-Labonté Virginie , Vigliotti Chloé , Tadic Zoran , Wehrle Beck , Lopez Philippe , Bapteste Eric , Lapointe François-Joseph , German Donovan P. , Herrel Anthony
Published: August 2022

Diet has been suggested to be an important driver of variation in microbiota composition in mammals. However, whether this is a more general phenomenon and how fast changes in gut microbiota occur with changes in diet remains poorly understood. Forty-nine years ago, ten lizards of the species Podarcis siculus were taken from the island of Pod Kopište and introduced onto the island of Pod Mrčaru (Croatia). The introduced population underwent a significant dietary shift, and their descendants became omnivorous (consuming up to 80% plant material during summer). Variation in their gut microbiota has never been investigated. To elucidate the possible impact on the gut microbiota of this rapid change in diet, we compared the microbiota (V4 region of the 16S rRNA gene) of P. siculus from Pod Mrčaru, Pod Kopište, and the mainland. In addition, we explored other drivers of variation in gut microbiota including insularity, the population of origin, and the year of sampling. Alpha-diversity analyses showed that the microbial diversity of omnivorous lizards was higher than the microbial diversity of insectivorous lizards. Moreover, omnivorous individuals harbored significantly more Methanobrevibacter. The gut microbial diversity of insectivorous lizards was nonetheless more heterogeneous. Insectivorous lizards on the mainland had different gut microbial communities than their counterparts on the island of Pod Kopište. Bacillus and Desulfovibrio were more abundant in the gut microbiota from insular lizards compared to mainland lizards. Finally, we showed that the population of origin was also an important driver of the composition of the gut microbiota. The dietary shift that occurred in the introduced population of P. siculus has had a detectable impact on the gut microbiota, but other factors such as insularity and the population of origin also contributed to differences in the gut microbial composition of these lizards, illustrating the multifactorial nature of the drivers of variation in gut microbiota. Overall, our data show that changes in gut microbiota may take place on ecological timescales. Yet, diet is only one of many factors driving variation in gut microbiota across populations.
Secondary Metabolites from the Endoparasitic Nematophagous Fungus Harposporium anguillulae YMF 1.01751
Authors: Dai Zebao , Gan Yang , Zhao Peiji , Li Guohong
Published: August 2022

Harposporium anguillulae, an endoparasitic nematophagous fungus (ENF), is a model fungus from which the genus Harposporium was established. It can infect nematodes via ingested conidia. In this paper, the morphology and nematode–fungus interaction between Panagrellus redivivus and H. anguillulae were observed by scanning electron microscopy (SEM). The secondary metabolites of H. anguillulae were also studied. Seven metabolites were purified and identified from an ethyl acetate extract of broth and a methanol extract of mycelium. These include a new polyketone 5-hydroxy-3-(hydroxymethyl)-6-methyl-2H-pyran-2-one (1) and six known metabolites (17R)-17-methylincisterol (2), eburicol (3), ergosterol peroxide (4), terpendole C (5), (3β,5α,9β,22E)-3,5-dihydroxy-ergosta-7,22-dien-6-one (6), and 5α,6β-epoxy-(22E,24R)-ergosta-8,22-diene- 3β,7α-diol (7). These metabolites were assayed for their activity against plant root-knot nematode, Meloidogyne incognita, and the results showed that terpendole C (5) had weak nematicidal activity but also that other compounds did not have evident activity at a concentration of 400 μg mL−1. Compound 1 exhibited an attractive effect towards P. redivivus.
Petrogenesis of the Early Cretaceous Zhouguan Granodiorite in Jiaodong Peninsula: Evidence from Mineralogy, Geochemistry, Geochronology, and Sr-Nd Isotopes
Authors: Chi Naijie , Han Zuozhen , Tian Ruicong , Liu Chuan’e , Shan Wei , Xiong Yuqiang , Li Zengsheng , Xie Yufeng , Li Min , Wang Xiufeng
Published: August 2022

The Jiaodong Peninsula is the most important gold mineralization area in China, and the formation of gold deposits is closely related to granitoids. The isotopic ages of the Early Cretaceous granodiorites in the northwestern Jiaodong Peninsula are concentrated in the range of 111~123 Ma, and are coeval with the formation of the gold deposits in the area. However, the studies on the geotectonic settings of the granodiorites, especially their petrogenesis and relationship with gold deposits in the northwestern Jiaodong Peninsula, are scarce. Based on field and petrographic observations, geochemistry, EPMA analysis, zircon U-Pb chronology, and Sr-Nd isotopes of the Early Cretaceous Zhouguan granodiorite in the Jiaodong area, the formation age of Zhouguan granodiorite is determined as 115 Ma ± 0.77 Ma; the analysis of EPMA shows that biotite is mainly composed of Fe-biotite and Mg-biotite, with its MgO content ranging from 9.797% to 11.635%. The crystallization temperature of biotite is in the range of 500 °C~625 °C and the emplacement depth of the rock mass is 3.98~8.71 km. The amphibole in the mass mainly includes magnesiohornblende, pargasite, and magnesiosadanagaite; among them, the former two are of crustal origin, while magnesiosadanagaite is of mantle origin. The crystallization pressure and depth of the former two are in the range of 0.75~3.02 kbar and 2.81~11.4 km, respectively, while the crystallization pressure and depth for the latter is 4.64 kbar and 17.53 km, respectively. The (87Sr/86Sr) values range from 0.710424 to 0.711074 and the (143Nd/144Nd) values range from 0.511530 to 0.511808. The parental magma of the Zhouguan granodiorite is highly oxidized with high-water content that is favorable for Au enrichment. Combined with the Nb-Y and Yb-Ta diagrams, a model describing the formation of Zhouguan granodiorite is proposed.
Study on the Phase Transition from Quartz to Coesite under High Temperature and High Pressure
Authors: Ren Dongsheng
Published: August 2022

Quartz is an important component of the Earth. In this study, experiments were conducted at temperatures between 600 to 700 °C, confining pressures between 1.5 and 1.8 GPa, and differential stress conditions. It was found that coesite production is closely related to differential stress, reaction time, and reaction temperature, with coesite formation being a multifactorial coupling process.
Secondary Minerals from Minothem Environments in Fragnè Mine (Turin, Italy): Preliminary Results
Authors: Galliano Yuri , Carbone Cristina , Balestra Valentina , Belmonte Donato , De Waele Jo
Published: August 2022

The Fragnè mine, located in the Lanzo valley in the municipality of Chialamberto (Turin, Piedmont Region), represented the most important regional site for Fe–Cu sulfide exploitation over a period of more than eighty years (1884–1965). The entire mining area is part of a structural complex in the Lower Piedmont Unit of the Western Alps, characterized by the presence of amphibolite, metabasite (“prasinite”), and metagabbroic rocks. In particular, the pyrite ore deposit occurs as massive mineralizations within interlayered metabasites and amphibolites. In this work, we describe secondary minerals and morphologies of minothems from the Fragnè mine that are found only in abandoned underground works, such as soda straws, normal and jelly stalactites and stalagmites, jellystones, columns, crusts, blisters, war-clubs, and hair, characterized by different mineralogical associations. All minothems were characterized by minerals formed during acid mine drainage (AMD) processes. Blisters are composed only of schwertmannite, war-clubs by schwertmannite, and goethite with low crystallinity and hair by epsomite and hexahydrite minerals. Jelly stalactites and stalagmites are characterized by schwertmannite often in association with bacteria, while solid stalactites and stalagmites are characterized by jarosite and goethite. The results indicate that the mineralogical transformation from schwertmannite to goethite observed in some minothems is probably due to aging processes of schwertmannite or local pH variations due to bacterial activity. On the basis of these results, we hypothesize that all the jelly samples, in association with strong bacterial activity, are slowly transformed into more solid goethite, and are thus precursors of goethite stalactites.
Geochronology, Geochemistry, and Lu-Hf Isotopic Compositions of Monzogranite Intrusion from the Chang’anpu Mo Deposit, NE China: Implications for Tectonic Setting and Mineralization
Authors: Zhang Jian , Yang Yanchen , Han Shijiong , Wutiepu Wukeyila
Published: August 2022

The Chang’anpu Molybdenum deposit occurs in the monzogranite intrusions in the Lesser Khingan Mountains-Zhangguangcai Mountains metallogenic belt. Previous work focused on the study of deposits, including geological characteristics, mineralization time, S-Pb isotope, etc. However, systematic petrogeochemical study of monzogranite intrusion and comparative analysis with other porphyry deposits in the region are lacking. Three monzogranite dating samples yield LA-ICP-MS zircon weighted mean 206Pb/238U ages of 174.7 ± 1.3 Ma, 174.9 ± 1.4 Ma, and 174.3 ± 1.8 Ma, respectively, indicating that the magmatism occurred in the middle Jurassic of Mesozoic. The 14 monzogranite samples show alkali rich and relatively high silica content (up to 84.39%) with the differentiation index (DI) ranges from 86 to 96, showing that monzogranite have been subjected to fractional crystallization during its evolution; the depletion of Ba, Sr, P, Nb, Ti, and Eu also indicates that the rock has undergone crystallization fractionation, the monzogranite belong to the highly fractionated I-type. Positive εHf(t) values (6.72–8.85) and young TDM2 (551–673 Ma) of the monzogranite indicate that the formation of Chang’anpu monzogranite intrusion is related to the partial melting of juvenile lower crust, originated from the Mesoproterozoic depleted mantle. The magmatism and related Mo mineralization in the Chang’anpu deposit occurred in an active continental margin setting associated with westward subduction of the Paleo-Pacific plate beneath the Eurasian plate.
Nicotine Inhibits the Cytotoxicity and Genotoxicity of NNK Mediated by CYP2A13 in BEAS-2B Cells
Authors: Sun Yulin , Wang Hongjuan , Chen Huan , Zhang Sen , Li Jun , Zhang Jingni , Tian Jianlu , Zhang Youyu , Hou Hongwei , Hu Qingyuan
Published: August 2022

Both tobacco-specific carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) and nicotine can be metabolized by cytochrome P450 2A13 (CYP2A13). Previous studies have shown that nicotine has a potential inhibitory effect on the toxicity of NNK. However, due to the lack of CYP2A13 activity in conventional lung cell lines, there had been no systematic in vitro investigation for the key target organ, the lung. Here, BEAS-2B cells stably expressing CYP2A13 (B-2A13 cells) were constructed to investigate the effects of nicotine on the cytotoxicity and genotoxicity of NNK. The results showed more sensitivity for NNK-induced cytotoxicity in B-2A13 cells than in BEAS-2B and B-vector cells. NNK significantly induced DNA damage, cell cycle arrest, and chromosomal damage in B-2A13 cells, but had no significant effect on BEAS-2B cells and the vector control cells. The combination of different concentration gradient of nicotine without cytotoxic effects and a single concentration of NNK reduced or even counteracted the cytotoxicity and multi-dimensional genotoxicity in a dose-dependent manner. In conclusion, CYP2A13 caused the cytotoxicity and genotoxicity of NNK in BEAS-2B cells, and the addition of nicotine could inhibit the toxicity of NNK.