bluuebunny/crossref_metadata_2025 huggingface dataset:
The Other Side of Fire in a Changing Environment: Evidence from a Mediterranean Country
Authors: Kalfas Dimitrios , Kalogiannidis Stavros , Chatzitheodoridis Fotios , Margaritis Nikolaos
Published: January 2024

In forested ecosystems all over the world, usually, fire is the main disturbance, and due to global climate change, its effects are worsening in many areas. Although fire impacts have been studied for many years, integrative analyses of their effects on various ecosystem services (ES) at different scales are uncommon. This study tries to assess the ecological role of fire in a changing environment, focusing on a Mediterranean country. Data were collected by the use of an online questionnaire in Greece, where the summer fires in the last decades have had significant impacts on the environment and the economy and, in many cases, there were many human and animal victims from them. The sample size of the survey was 384 workers in the primary production sector from all over the country. The study showed that fire has several effects on animal husbandry, the quality of soil nutrients and fertility, the overall vegetation cover, and on general biodiversity. It seems that the degree to which fire has an effect on ecosystem components depends on the intensity, frequency, and length of the fires. Additionally, the frequency, intensity, and length of fire affect the impacts of fire on herbaceous plant, woody vegetation, soil physical qualities, and on the different animals’ habitats.
Cross-Linked Hyaluronan Derivatives in the Delivery of Phycocyanin
Authors: Terracina Francesca , Saletti Mario , Paolino Marco , Venditti Jacopo , Giuliani Germano , Bonechi Claudia , Licciardi Mariano , Cappelli Andrea
Published: January 2024

An easy and viable crosslinking technology, based on the “click-chemistry” reaction copper(I)-catalyzed azide-alkyne 1,3-dipolar cycloaddition (click-crosslinking), was applied to graft copolymers of medium molecular weight (i.e., 270 kDa) hyaluronic acid (HA) grafted with ferulic acid (FA) residues bearing clickable propargyl groups, as well as caffeic acid derivatives bearing azido-terminated oligo(ethylene glycol) side chains. The obtained crosslinked materials were characterized from the point of view of their structure and aggregation liability to form hydrogels in a water environment. The most promising materials showed interesting loading capability regarding the antioxidant agent phycocyanin (PC). Two novel materials complexes (namely HA(270)-FA-TEGEC-CL-20/PC and HA(270)-FA-HEGEC-CL-20/PC) were obtained with a drug-to-material ratio of 1:2 (w/w). Zeta potential measurements of the new complexes (−1.23 mV for HA(270)-FA-TEGEC-CL-20/PC and −1.73 mV for HA(270)-FA-HEGEC-CL-20/PC) showed alterations compared to the zeta potential values of the materials on their own, suggesting the achievement of drug–material interactions. According to the in vitro dissolution studies carried out in different conditions, novel drug delivery systems (DDSs) were obtained with a variety of characteristics depending on the desired route of administration and, consequently, on the pH of the surrounding environment, thanks to the complexation of phycocyanin with these two new crosslinked materials. Both complexes showed excellent potential for providing a controlled/prolonged release of the active pharmaceutical ingredient (API). They also increased the amount of drug that reach the target location, enabling pH-dependent release. Importantly, as demonstrated by the DPPH free radical scavenging assay, the complexation process, involving freezing and freeze-drying, showed no adverse effects on the antioxidant activity of phycocyanin. This activity was preserved in the two novel materials and followed a concentration-dependent pattern similar to pure PC.
Genealogical Violence: Mormon (Mis)Appropriation of Māori Cultural Memory through Falsification of Whakapapa
Authors: Simon Hemopereki
Published: January 2024

The study examines how members of the historically white possessive and supremacist Church of Jesus Christ of Latter-day Saints in the United States (mis)appropriated Māori genealogy, known as whakapapa. The Mormon use of whakapapa to promote Mormon cultural memory and narratives perpetuates settler/invader colonialism and white supremacy, as this paper shows. The research discusses Church racism against Native Americans and Pacific Peoples. This paper uses Anthropologist Thomas Murphy’s scholarship to demonstrate how problematic the Book of Mormon’s religio-colonial identity of Lamanites is for these groups. Application of Aileen Moreton-Robinson’s white possessive doctrine and Hemopereki Simon’s adaptation to cover Church-Indigenous relations and the salvation contract is discussed. We explore collective and cultural memory, and discuss key Māori concepts like Mana, Taonga, Tapu, and Whakapapa. A brief review of LDS scholar Louis C. Midgley’s views on Church culture, including Herewini Jone’s whakapapa wānanga, is followed by a discussion of Māori cultural considerations and issues. The paper concludes that the alteration perpetuates settler/invader colonialism and Pacific peoples’ racialization and white supremacy. Genetic science and human migration studies contradict Mormon identity narratives and suggest the BOM is spiritual rather than historical. Finally, the paper suggests promoting intercultural engagement on Mormon (mis)appropriation of taonga Māori.
High-Impact Teaching Practices in Higher Education: Understanding Barriers, Concerns, and Obstacles to Their Adoption
Authors: VanWyngaarden Kristin , Pelton Julie A. , Oquendo Pamela Martínez , Moore Christopher
Published: January 2024

This research explores the barriers, concerns, and obstacles undergraduate STEM educators face when implementing high-impact teaching practices (HIPs), the application of which may improve student learning outcomes. Because our study took place during the COVID-19 pandemic, our results also shed light on the unique challenges of utilizing HIPs in asynchronous online-learning environments. Thirteen undergraduate instructors were interviewed about their current teaching practices in order to identify barriers to or support for adopting HIPs. Data collected through semi-structured interviews revealed administrative and financial restraints as barriers to effective teaching which have been found in previous research. A number of new and unique obstacles emerged out of teaching remotely or online during the pandemic, including a heightened concern over the instructor’s ability to connect with students and engage in the best teaching practices. This research extends our current understanding of barriers and concerns about adopting HIPs in undergraduate STEM courses because of the unique perceived threats that emerged during the pandemic. We identify strategies to equip faculty with the support they need to provide equitable learning experiences, including access to consultants who support curriculum development and implementation in the classroom, ongoing educational coaching, and increased access to professional-development opportunities and a community of inquiry to discuss teaching strategies.
Selenium Seed Priming and Biostimulation Influence the Seed Germination and Seedling Morphology of Jalapeño (Capsicum annuum L.)
Authors: de los Ángeles Sariñana-Navarrete María , Benavides-Mendoza Adalberto , González-Morales Susana , Juárez-Maldonado Antonio , Preciado-Rangel Pablo , Sánchez-Chávez Esteban , Cadenas-Pliego Gregorio , Antonio-Bautista Adriana , Morelos-Moreno Álvaro
Published: January 2024

The priming of seeds is shown as a viable technique to improve germination, the growth of the radicle and plumule, and the seedling vigor index, which gives rise to seedlings with higher quality and tolerance to environmental growing conditions. Sodium selenite (Na2SeO3) and selenium nanoparticles (nSe) were used as priming media and postgermination biostimulation in seeds of jalapeño pepper, in concentrations of 1, 5, 10, 15, 20, 25, 30, 35, 40, and 45 mg L−1 for the two Se species, and control treatment. This research aimed to determine the priming response of jalapeño pepper regarding the germination percent, germination speed index, radicle length, plumule length, fresh weight, and seedling vigor index. The stimulation and phytotoxicity thresholds were also computed. The results showed a percentage of germination greater than 80% in all concentrations evaluated. Most variables of jalapeño pepper presented stimulation responses at Na2SeO3 doses lower than 5 mg L−1 and nSe doses lower than 15 mg L−1. The higher daily germination was favored by nSe on the fifth day compared to the sixth day of Na2SeO3; in addition, the higher cumulative germination occurred on the sixth day with nSe and on the eighth day with Na2SeO3. The use of low Na2SeO3 concentrations positively favors germination and the morphological traits of the shoots. Likewise, the use of Se in nanometric form was friendlier, that is, the degree of tolerance to Se was higher.
Potential Effects of Long-Term Exposure to Air Pollution on Dementia: A Longitudinal Analysis in American Indians Aged 55 Years and Older
Authors: Zhu Yachen , Shi Yuxi , Bartell Scott M. , Corrada Maria M. , Manson Spero M. , O’Connell Joan , Jiang Luohua
Published: January 2024

(1) Background: American Indians are disproportionately affected by air pollution, an important risk factor for dementia. However, few studies have investigated the effects of air pollution on the risk of dementia among American Indians. (2) Methods: This retrospective cohort study included a total of 26,871 American Indians who were 55+ years old in 2007, with an average follow-up of 3.67 years. County-level average air pollution data were downloaded from land-use regression models. All-cause dementia was identified using ICD-9 diagnostic codes from the Indian Health Service’s (IHS) National Data Warehouse and related administrative databases. Cox models were employed to examine the association of air pollution with dementia incidence, adjusting for co-exposures and potential confounders. (3) Results: The average PM2.5 levels in the IHS counties were lower than those in all US counties, while the mean O3 levels in the IHS counties were higher than the US counties. Multivariable Cox regressions revealed a positive association between dementia and county-level O3 with a hazard ratio of 1.24 (95% CI: 1.02–1.50) per 1 ppb standardized O3. PM2.5 and NO2 were not associated with dementia risk after adjusting for all covariates. (4) Conclusions: O3 is associated with a higher risk of dementia among American Indians.
Predictors of Patient Engagement in Telehealth-Delivered Tobacco Cessation Treatment during the COVID-19 Pandemic
Authors: Jagielo Annemarie D. , Chieng Amy , Tran Cindy , Pirkl Amy , Cao-Nasalga Ann , Bragg Ashley , Mirkin Rachelle , Prochaska Judith J.
Published: January 2024

Smoking causes one in three cancer deaths and may worsen COVID-19 outcomes. Telehealth tobacco cessation treatment is offered as a covered benefit for patients at the Stanford Cancer Center. We examined predictors of engagement during the COVID-19 pandemic. Data were abstracted from the Electronic Health Record between 3/17/20 (start of pandemic shelter-in-place) and 9/20/22, including patient tobacco use, demographics, and engagement in cessation treatment. Importance of quitting tobacco was obtained for a subset (53%). During the first 2.5 years of the pandemic, 2595 patients were identified as recently using tobacco, and 1571 patients were contacted (61%). Of the 1313 patients still using tobacco (40% women, mean age 59, 66% White, 13% Hispanic), 448 (34%) enrolled in treatment. Patient engagement was greater in pandemic year 1 (42%) than in year 2 (28%) and year 3 (19%). Women (41%) engaged more than men (30%). Patients aged 36–45 (39%), 46–55 (43%), 56–65 (37%), and 66–75 (33%) engaged more than patients aged 18–35 (18%) and >75 (21%). Hispanic/Latinx patients (42%) engaged more than non-Hispanic/Latinx patients (33%). Engagement was not statistically significantly related to patient race. Perceived importance of quitting tobacco was significantly lower in pandemic year 1 than year 2 or 3. Nearly one in three cancer patients engaged in telehealth cessation treatment during the COVID-19 pandemic. Engagement was greater earlier in the pandemic, among women, Hispanic/Latinx individuals, and patients aged 36 to 75. Sheltering-in-place, rather than greater perceived risk, may have facilitated patient engagement in tobacco cessation treatment.
High Doses of ANA12 Improve Phenobarbital Efficacy in a Model of Neonatal Post-Ischemic Seizures
Authors: Vyas Preeti , Chaturvedi Ira , Hwang Yun , Scafidi Joseph , Kadam Shilpa D. , Stafstrom Carl E.
Published: January 2024

Phenobarbital (PB) remains the first-line medication for neonatal seizures. Yet, seizures in many newborns, particularly those associated with perinatal ischemia, are resistant to PB. Previous animal studies have shown that in postnatal day P7 mice pups with ischemic stroke induced by unilateral carotid ligation, the tyrosine receptor kinase B (TrkB) antagonist ANA12 (N-[2-[[(hexahydro-2-oxo-1H-azepin-3-yl)amino]carbonyl]phenyl]-benzo[b]thiophene-2-carboxamide, 5 mg/kg) improved the efficacy of PB in reducing seizure occurrence. To meet optimal standards of effectiveness, a wider range of ANA12 doses must be tested. Here, using the unilateral carotid ligation model, we tested the effectiveness of higher doses of ANA12 (10 and 20 mg/kg) on the ability of PB to reduce seizure burden, ameliorate cell death (assessed by Fluoro-Jade staining), and affect neurodevelopment (righting reflex, negative geotaxis test, open field test). We found that a single dose of ANA12 (10 or 20 mg/kg) given 1 h after unilateral carotid ligation in P7 pups reduced seizure burden and neocortical and striatal neuron death without impairing developmental reflexes. In conclusion, ANA12 at a range of doses (10–20 mg/kg) enhanced PB effectiveness for the treatment of perinatal ischemia-related seizures, suggesting that this agent might be a clinically safe and effective adjunctive agent for the treatment of pharmacoresistant neonatal seizures.
Phosphatidyl Ethanolamine Binding Protein FLOWERING LOCUS T-like 12 (OsFTL12) Regulates the Rice Heading Date under Different Day-Length Conditions
Authors: Huang Yongxiang , Guo Jianfu , Sun Dayuan , Guo Zhenhua , Zheng Zihao , Wang Ping , Hong Yanbin , Liu Hao
Published: January 2024

Plant FLOWERING LOCUS T-Like (FTL) genes often redundantly duplicate on chromosomes and functionally diverge to modulate reproductive traits. Rice harbors thirteen FTL genes, the functions of which are still not clear, except for the Hd3a and RFT genes. Here, we identified the molecular detail of OsFTL12 in rice reproductive stage. OsFTL12 encoding protein contained PEBP domain and localized into the nucleus, which transcripts specifically expressed in the shoot and leaf blade with high abundance. Further GUS-staining results show the OsFTL12 promoter activity highly expressed in the leaf and stem. OsFTL12 knock-out concurrently exhibited early flowering phenotype under the short- and long-day conditions as compared with wild-type and over-expression plants, which independently regulates flowering without an involved Hd1/Hd3a and Ehd1/RFT pathway. Further, an AT-hook protein OsATH1 was identified to act as upstream regulator of OsFTL12, as the knock-out OsATH1 elevated the OsFTL12 expression by modifying Histone H3 acetylation abundance. According to the dissection of OsFTL12 molecular functions, our study expanded the roles intellectual function of OsFTL12 in the mediating of a rice heading date.
Chronic Variable Stress and Cafeteria Diet Combination Exacerbate Microglia and c-fos Activation but Not Experimental Anxiety or Depression in a Menopause Model
Authors: Vega-Rivera Nelly Maritza , Estrada-Camarena Erika , Azpilcueta-Morales Gabriel , Cervantes-Anaya Nancy , Treviño Samuel , Becerril-Villanueva Enrique , López-Rubalcava Carolina
Published: January 2024

The menopause transition is a vulnerable period for developing both psychiatric and metabolic disorders, and both can be enhanced by stressful events worsening their effects. The present study aimed to evaluate whether a cafeteria diet (CAF) combined with chronic variable stress (CVS) exacerbates anxious- or depressive-like behavior and neuronal activation, cell proliferation and survival, and microglia activation in middle-aged ovariectomized (OVX) rats. In addition, body weight, lipid profile, insulin resistance, and corticosterone as an index of metabolic changes or hypothalamus–pituitary–adrenal (HPA) axis activation, and the serum pro-inflammatory cytokines IL-6, IL-β, and TNFα were measured. A CAF diet increased body weight, lipid profile, and insulin resistance. CVS increased corticosterone and reduced HDL. A CAF produced anxiety-like behaviors, whereas CVS induced depressive-like behaviors. CVS increased serum TNFα independently of diet. A CAF and CVS separately enhanced the percentage of Iba-positive cells in the hippocampus; the combination of factors further increased Iba-positive cells in the ventral hippocampus. A CAF and CVS increased the c-fos-positive cells in the hippocampus; the combination of factors increased the number of positive cells expressing c-fos in the ventral hippocampus even more. The combination of a CAF and CVS generates a slight neuroinflammation process and neuronal activation in a hippocampal region-specific manner and differentially affects the behavior.
New Opportunities in the Systemic Treatment of Hepatocellular Carcinoma—Today and Tomorrow
Authors: Becht Rafał , Kiełbowski Kajetan , Wasilewicz Michał P.
Published: January 2024

Hepatocellular carcinoma (HCC) is the most common primary liver cancer. Liver cirrhosis, hepatitis B, hepatitis C, and non-alcoholic fatty liver disease represent major risk factors of HCC. Multiple different treatment options are available, depending on the Barcelona Clinic Liver Cancer (BCLC) algorithm. Systemic treatment is reserved for certain patients in stages B and C, who will not benefit from regional treatment methods. In the last fifteen years, the arsenal of available therapeutics has largely expanded, which improved treatment outcomes. Nevertheless, not all patients respond to these agents and novel combinations and drugs are needed. In this review, we aim to summarize the pathway of trials investigating the safety and efficacy of targeted therapeutics and immunotherapies since the introduction of sorafenib. Furthermore, we discuss the current evidence regarding resistance mechanisms and potential novel targets in the treatment of advanced HCC.
Defining the Role of the miR-145—KLF4—αSMA Axis in Mitral Valvular Interstitial Cell Activation in Myxomatous Mitral Valve Prolapse Using the Canine Model
Authors: Yang Vicky K. , Moyer Nicole , Zhou Runzi , Carnevale Sally Z. , Meola Dawn M. , Robinson Sally R. , Li Guoping , Das Saumya
Published: January 2024

Mitral valve prolapse (MVP) is a common valvular disease, affecting 2–3% of the adult human population and is a degenerative condition. A total of 5–10% of the afflicted will develop severe mitral regurgitation, cardiac dysfunction, congestive heart failure, and sudden cardiac death. Naturally occurring myxomatous MVP in dogs closely resembles MVP in humans structurally, and functional consequences are similar. In both species, valvular interstitial cells (VICs) in affected valves exhibit phenotype consistent with activated myofibroblasts with increased alpha-smooth muscle actin (αSMA) expression. Using VICs collected from normal and MVP-affected valves of dogs, we analyzed the miRNA expression profile of the cells and their associated small extracellular vesicles (sEV) using RNA sequencing to understand the role of non-coding RNAs and sEV in MVP pathogenesis. miR-145 was shown to be upregulated in both the affected VICs and sEV, and overexpression of miR-145 by mimic transfection in quiescent VIC recapitulates the activated myofibroblastic phenotype. Concurrently, KLF4 expression was noted to be suppressed by miR-145, confirming the miR-145—KLF4—αSMA axis. Targeting this axis may serve as a potential therapy in controlling pathologic abnormalities found in MVP valves.
Sodium Channel β Subunits—An Additional Element in Animal Tetrodotoxin Resistance?
Authors: Seneci Lorenzo , Mikheyev Alexander S.
Published: January 2024

Tetrodotoxin (TTX) is a neurotoxic molecule used by many animals for defense and/or predation, as well as an important biomedical tool. Its ubiquity as a defensive agent has led to repeated independent evolution of tetrodotoxin resistance in animals. TTX binds to voltage-gated sodium channels (VGSC) consisting of α and β subunits. Virtually all studies investigating the mechanisms behind TTX resistance have focused on the α subunit of voltage-gated sodium channels, where tetrodotoxin binds. However, the possibility of β subunits also contributing to tetrodotoxin resistance was never explored, though these subunits act in concert. In this study, we present preliminary evidence suggesting a potential role of β subunits in the evolution of TTX resistance. We gathered mRNA sequences for all β subunit types found in vertebrates across 12 species (three TTX-resistant and nine TTX-sensitive) and tested for signatures of positive selection with a maximum likelihood approach. Our results revealed several sites experiencing positive selection in TTX-resistant taxa, though none were exclusive to those species in subunit β1, which forms a complex with the main physiological target of TTX (VGSC Nav1.4). While experimental data validating these findings would be necessary, this work suggests that deeper investigation into β subunits as potential players in tetrodotoxin resistance may be worthwhile.
Catalase (CAT) Gene Family in Oil Palm (Elaeis guineensis Jacq.): Genome-Wide Identification, Analysis, and Expression Profile in Response to Abiotic Stress
Authors: Zhou Lixia , John Martin Jerome Jeyakumar , Li Rui , Zeng Xianhai , Wu Qiufei , Li Qihong , Fu Dengqiang , Li Xinyu , Liu Xiaoyu , Ye Jianqiu , Cao Hongxing
Published: January 2024

Catalases (CATs) play crucial roles in scavenging H2O2 from reactive oxygen species, controlling the growth and development of plants. So far, genome-wide identification and characterization of CAT genes in oil palm have not been reported. In the present study, five EgCAT genes were obtained through a genome-wide identification approach. Phylogenetic analysis divided them into two subfamilies, with closer genes sharing similar structures. Gene structure and conserved motif analysis demonstrated the conserved nature of intron/exon organization and motifs among the EgCAT genes. Several cis-acting elements related to hormone, stress, and defense responses were identified in the promoter regions of EgCATs. Tissue-specific expression of EgCAT genes in five different tissues of oil palm was also revealed by heatmap analysis using the available transcriptome data. Stress-responsive expression analysis showed that five EgCAT genes were significantly expressed under cold, drought, and salinity stress conditions. Collectively, this study provided valuable information on the oil palm CAT gene family and the validated EgCAT genes can be used as potential candidates for improving abiotic stress tolerance in oil palm and other related crops.
The Modulation of Phospho-Extracellular Signal-Regulated Kinase and Phospho-Protein Kinase B Signaling Pathways plus Activity of Macrophage-Stimulating Protein Contribute to the Protective Effect of Stachydrine on Acetaminophen-Induced Liver Injury
Authors: Liu Fu-Chao , Yu Huang-Ping , Lee Hung-Chen , Chen Chun-Yu , Liao Chia-Chih
Published: January 2024

Stachydrine, a prominent bioactive alkaloid derived from Leonurus heterophyllus, is a significant herb in traditional medicine. It has been noted for its anti-inflammatory and antioxidant characteristics. Consequently, we conducted a study of its hepatoprotective effect and the fundamental mechanisms involved in acetaminophen (APAP)-induced liver injury, utilizing a mouse model. Mice were intraperitoneally administered a hepatotoxic dose of APAP (300 mg/kg). Thirty minutes after APAP administration, mice were treated with different concentrations of stachydrine (0, 2.5, 5, and 10 mg/kg). Animals were sacrificed 16 h after APAP injection for serum and liver tissue assays. APAP overdose significantly elevated the serum alanine transferase levels, hepatic pro-inflammatory cytokines, malondialdehyde activity, phospho-extracellular signal-regulated kinase (ERK), phospho-protein kinase B (AKT), and macrophage-stimulating protein expression. Stachydrine treatment significantly decreased these parameters in mice with APAP-induced liver damage. Our results suggest that stachydrine may be a promising beneficial target in the prevention of APAP-induced liver damage through attenuation of the inflammatory response, inhibition of the ERK and AKT pathways, and expression of macrophage-stimulating proteins.
Unraveling the Role of Ras Homolog Enriched in Brain (Rheb1 and Rheb2): Bridging Neuronal Dynamics and Cancer Pathogenesis through Mechanistic Target of Rapamycin Signaling
Authors: Rahman Mostafizur , Nguyen Tuan Minh , Lee Gi Jeong , Kim Boram , Park Mi Kyung , Lee Chang Hoon
Published: January 2024

Ras homolog enriched in brain (Rheb1 and Rheb2), small GTPases, play a crucial role in regulating neuronal activity and have gained attention for their implications in cancer development, particularly in breast cancer. This study delves into the intricate connection between the multifaceted functions of Rheb1 in neurons and cancer, with a specific focus on the mTOR pathway. It aims to elucidate Rheb1’s involvement in pivotal cellular processes such as proliferation, apoptosis resistance, migration, invasion, metastasis, and inflammatory responses while acknowledging that Rheb2 has not been extensively studied. Despite the recognized associations, a comprehensive understanding of the intricate interplay between Rheb1 and Rheb2 and their roles in both nerve and cancer remains elusive. This review consolidates current knowledge regarding the impact of Rheb1 on cancer hallmarks and explores the potential of Rheb1 as a therapeutic target in cancer treatment. It emphasizes the necessity for a deeper comprehension of the molecular mechanisms underlying Rheb1-mediated oncogenic processes, underscoring the existing gaps in our understanding. Additionally, the review highlights the exploration of Rheb1 inhibitors as a promising avenue for cancer therapy. By shedding light on the complicated roles between Rheb1/Rheb2 and cancer, this study provides valuable insights to the scientific community. These insights are instrumental in guiding the identification of novel targets and advancing the development of effective therapeutic strategies for treating cancer.
Transcriptomic and Metabolomic Analyses Reveal the Role of Phenylalanine Metabolism in the Maize Response to Stalk Rot Caused by Fusarium proliferatum
Authors: Sun Jianjun , Wang Yanzhao , Zhang Xingrui , Cheng Zeqiang , Song Yinghui , Li Huimin , Wang Na , Liu Shen , Cao Zijia , Li Hongxia , Zheng Wanying , Duan Canxing , Cao Yanyong
Published: January 2024

Stalk rot is a prevalent disease of maize (Zea mays L.) that severely affects maize yield and quality worldwide. The ascomycete fungus Fusarium spp. is the most common pathogen of maize stalk rot. At present, the molecular mechanism of Fusarium proliferation during the maize stalk infection that causes maize stalk rot has rarely been reported. In this study, we investigated the response of maize to F. proliferatum infestation by analyzing the phenotypic, transcriptomic, and metabolomic data of inbred lines ZC17 (resistant) and CH72 (susceptible) with different levels of resistance to stalk rot. Physiological and phenotypic results showed that the infection CH72 was significantly more severe than ZC17 after inoculation. Transcriptome analysis showed that after inoculation, the number of differentially expressed genes (DEGs) was higher in CH72 than in ZC17. Nearly half of these DEGs showed the same expression trend in the two inbred lines. Functional annotation and enrichment analyses indicated that the major pathways enriched for DEGs and DEMs included the biosynthesis of plant secondary metabolites, phenylalanine metabolism, biosynthesis of plant hormones, and plant–pathogen interactions. The comprehensive analysis of transcriptome and metabolome data indicated that phenylalanine metabolism and the phenylalanine, tyrosine, and tryptophan biosynthesis pathways played a crucial role in maize resistance to F. proliferatum infection. In addition, a transcription factor (TF) analysis of the DEGs showed that several TF families, including MYB, bHLH, NAC, and WRKY, were significantly activated after inoculation, suggesting that these TFs play important roles in the molecular regulatory network of maize disease resistance. The findings of this study provide valuable insights into the molecular basis of the response of maize to Fusarium proliferatum infection and highlight the importance of combining multiple approaches, such as phenotyping, transcriptomics, and metabolomics, to gain a comprehensive understanding of plant–pathogen interactions.
Anti-Inflammatory Effect of Synaptamide in Ischemic Acute Kidney Injury and the Role of G-Protein-Coupled Receptor 110
Authors: Brezgunova Anna A. , Andrianova Nadezda V. , Saidova Aleena A. , Potashnikova Daria M. , Abramicheva Polina A. , Manskikh Vasily N. , Mariasina Sofia S. , Pevzner Irina B. , Zorova Ljubava D. , Manzhulo Igor V. , Zorov Dmitry B. , Plotnikov Egor Y.
Published: January 2024

The development of drugs for the treatment of acute kidney injury (AKI) that could suppress the excessive inflammatory response in damaged kidneys is an important clinical challenge. Recently, synaptamide (N-docosahexaenoylethanolamine) has been shown to exert anti-inflammatory and neurogenic properties. The aim of this study was to investigate the anti-inflammatory effect of synaptamide in ischemic AKI. For this purpose, we analyzed the expression of inflammatory mediators and the infiltration of different leukocyte populations into the kidney after injury, evaluated the expression of the putative synaptamide receptor G-protein-coupled receptor 110 (GPR110), and isolated a population of CD11b/c+ cells mainly representing neutrophils and macrophages using cell sorting. We also evaluated the severity of AKI during synaptamide therapy and the serum metabolic profile. We demonstrated that synaptamide reduced the level of pro-inflammatory interleukins and the expression of integrin CD11a in kidney tissue after injury. We found that the administration of synaptamide increased the expression of its receptor GPR110 in both total kidney tissue and renal CD11b/c+ cells that was associated with the reduced production of pro-inflammatory interleukins in these cells. Thus, we demonstrated that synaptamide therapy mitigates the inflammatory response in kidney tissue during ischemic AKI, which can be achieved through GPR110 signaling in neutrophils and a reduction in these cells’ pro-inflammatory interleukin production.
Preparative and Catalytic Properties of MoVI Mononuclear and Metallosupramolecular Coordination Assemblies Bearing Hydrazonato Ligands
Authors: Mandarić Mirna , Topić Edi , Agustin Dominique , Pisk Jana , Vrdoljak Višnja
Published: January 2024

A series of polynuclear, dinuclear, and mononuclear Mo(VI) complexes were synthesized with the hydrazonato ligands derived from 5-methoxysalicylaldehyde and the corresponding hydrazides (isonicotinic hydrazide (H2L1), nicotinic hydrazide (H2L2), 2-aminobenzhydrazide (H2L3), or 4-aminobenzhydrazide (H2L4)). The metallosupramolecular compounds obtained from non-coordinating solvents, [MoO2(L1,2)]n (1 and 2) and [MoO2(L3,4)]2 (3 and 4), formed infinite structures and metallacycles, respectively. By blocking two coordination sites with cis-dioxo ligands, the molybdenum centers have three coordination sites occupied by the ONO donor atoms from the rigid hydrazone ligands and one by the N atom of pyridyl or amine-functionalized ligand subcomponents from the neighboring Mo building units. The reaction in methanol afforded the mononuclear analogs [MoO2(L1-4)(MeOH)] (1a–4a) with additional monodentate MeOH ligands. All isolated complexes were tested as catalysts for cyclooctene epoxidation using tert-butyl hydroperoxide (TBHP) as an oxidant in water. The impact of the structure and ligand lability on the catalytic efficiency in homogeneous cyclooctene epoxidation was elucidated based on theoretical considerations. Thus, dinuclear assemblies exhibited better catalytic activity than mononuclear or polynuclear complexes.
Experimental Investigation to Optimize the Manufacturing Parameters of Ankle–Foot Orthoses Using Composite and Titanium Nanoparticles
Authors: Khalaf Najlaa J. , Ben Amor Sabrine , Louhichi Borhen , Chiad Jumaa S. , Seibi Abdennour
Published: January 2024

The optimum structural characteristics of lamination materials used in the fabrication of prosthetic and orthotic parts were investigated in this work. Optimization was chosen based on high yields, ultimate stresses, and bending stress properties. The ideal materials were determined through the use of an RSM (response surface methodology) which considers three factors: Perlon reinforcement, a layer of glass fiber, and the percentage of titanium nanoparticles combined with the matrix laminating resin. The RSM approach suggests thirteen samples by manipulating two variables: the Ti nano percentage and the number of Perlon layers. Laminating materials, defined by RSM methods and treated with a vacuum system, were submitted to a series of tests. The ideal lamination material was compared with the laminations from the initial study through the use of tensile, flexural, and fatigue testing according to ASTM standards. Tests carried out using version 10.0.2 of Design Expert software showed that, compared with the 12 other laminations, the one with 10 Perlon layers and 0.75 percent Ti nano had the highest overall yield and ultimate and bending loads. Fatigue eventually showed that stamina tension constraints were applied for optimal lamination, compared to ten Perlon lamination layers. We additionally tested the fatigue life of the best material and compared it with the available materials used at prosthetics and orthotics centers.
A CNN Hyperparameters Optimization Based on Particle Swarm Optimization for Mammography Breast Cancer Classification
Authors: Aguerchi Khadija , Jabrane Younes , Habba Maryam , El Hassani Amir Hajjam
Published: January 2024

Breast cancer is considered one of the most-common types of cancers among females in the world, with a high mortality rate. Medical imaging is still one of the most-reliable tools to detect breast cancer. Unfortunately, manual image detection takes much time. This paper proposes a new deep learning method based on Convolutional Neural Networks (CNNs). Convolutional Neural Networks are widely used for image classification. However, the determination process for accurate hyperparameters and architectures is still a challenging task. In this work, a highly accurate CNN model to detect breast cancer by mammography was developed. The proposed method is based on the Particle Swarm Optimization (PSO) algorithm in order to look for suitable hyperparameters and the architecture for the CNN model. The CNN model using PSO achieved success rates of 98.23% and 97.98% on the DDSM and MIAS datasets, respectively. The experimental results proved that the proposed CNN model gave the best accuracy values in comparison with other studies in the field. As a result, CNN models for mammography classification can now be created automatically. The proposed method can be considered as a powerful technique for breast cancer prediction.
AI-Enhanced Blood Cell Recognition and Analysis: Advancing Traditional Microscopy with the Web-Based Platform IKOSA
Authors: Campos-Medina Manuel , Blumer Aiden , Kraus-Füreder Patrick , Mayrhofer-Reinhartshuber Michael , Kainz Philipp , Schmid Johannes A.
Published: January 2024

Microscopy of stained blood smears is still a ubiquitous technique in pathology. It is often used in addition to automated electronic counters or flow cytometers to evaluate leukocytes and their morphologies in a rather simple manner and has low requirements for resources and equipment. However, despite the constant advances in microscopy, computer science, and pathology, it still usually follows the traditional approach of manual assessment by humans. We aimed to extend this technique using AI-based automated cell recognition methods while maintaining its technical simplicity. Using the web platform IKOSA, we developed an AI-based workflow to segment and identify all blood cells in DAPI-Giemsa co-stained blood smears. Thereby, we could automatically detect and classify neutrophils (young and segmented), lymphocytes, eosinophils, and monocytes, in addition to erythrocytes and platelets, in contrast to previously published algorithms, which usually focus on only one type of blood cell. Furthermore, our method delivers quantitative measurements, unattainable by the classical method or formerly published AI techniques, and it provides more sophisticated analyses based on entropy or gray-level co-occurrence matrices (GLCMs), which have the potential to monitor changes in internal cellular structures associated with disease states or responses to treatment. We conclude that AI-based automated blood cell evaluation has the potential to facilitate and improve routine diagnostics by adding quantitative shape and structure parameters to simple leukocyte counts of classical analysis.
Referential Salience in French and Mandarin Chinese: Influence of Syntactic, Semantic and Textual Factors
Authors: Hou Jiaqi , Landragin Frédéric
Published: January 2024

In this article, we propose a multifactorial approach to salience analysis, examining the influence of five factors on the salience of referential entities in discourse. Significance tests and Cramer’s V tests were conducted to analyze textual data obtained through manual annotation of four text excerpts in French and in Chinese. The results show that almost all the factors have a significant influence on referents’ salience (except the animacy factor in one of the excerpts). While it seems difficult to predict a fixed ranking of salience factors, which depends more on textual characteristics than on differences between the two languages, the different values of each factor under investigation show an identical behavior in terms of the positive/negative contribution to salience. The results also suggest that some factors (syntactic function and syntactic parallelism) may have a more stable influence on referents’ salience than other factors (animacy, mobility, and main character), potentially constrained by textual properties such as the main character’s nature, its number of occurrences, and the possible existence of competing protagonists.
Spanish Loyalty and English Prestige in the Linguistic Landscape of Ciudad Juárez, Mexico
Authors: Mazzaro Natalia , Minjarez Oppenheimer Natalia , González de Anda Raquel
Published: January 2024

Linguistic landscape (LL) studies in bilingual regions can reveal power dynamics between two languages, providing important information regarding their status and vitality. To analyze the relationship between Spanish and English in Ciudad Juárez, a city on the south side of the U.S.-Mexico border, we collected 1649 pictures of public signs in several sections of the city, whose “wellness levels” differ from each other. Pictures were coded for several factors, including language choice, business type, sign type, and the main and informative section, amongst others. Results show that while Spanish is the most frequently used language, English has a strong presence in the LL of Ciudad Juárez. The main factor affecting language choice is “business type”. Certain businesses within the “beauty” category tend to favor the use of English, while businesses within the “home” category favor the use of Spanish. An analysis of socio-economic status (SES) and language choice revealed a direct relationship between them: English is favored in high-income neighborhoods, while Spanish is favored used in low-income areas. The analysis of the main and informative sections on signs further confirmed the prestige assigned to English, which appears mostly in the main and most prominent sections of a sign. Our research shows that although Spanish vitality in Ciudad Juárez is strong, English is used in advertising because it is prestigious and increases the value of products and services, making them more appealing to shoppers.
How Relevant Is the Sentence Unit to Accessing Implicit Meaning?
Authors: Pozniak Céline , Beyssade Claire , Roussarie Laurent , Godart-Wendling Béatrice
Published: January 2024

This paper examines the relevance of the sentence concept to the understanding of three types of implicitness (presupposition, conversational implicatures, irony). Our experimental protocol involved 105 children (aged 6 to 11) and 82 adults who were asked to read short texts composed of a context about some characters and a target sentence conveying one of the three implicit contents. After reading, children and adults had to answer a comprehension yes-no question and indicate the segments from the text that helped them answer the question. Results showed a difference between the three types of implicitness, with presupposition being detected and understood at a subsentential level, whereas implicatures and irony come under extrasentential level requiring the context to be taken into account. Referring to sentence as a unit of meaning does not seem relevant as soon as understanding is not limited to the literal meaning of what is written, but also concerns what is meant by the text.
Mn(II)-Activated Zero-Dimensional Zinc(II)-Based Metal Halide Hybrids with Near-Unity Photoluminescence Quantum Yield
Authors: Peng Chengyu , Wei Jiazheng , Duan Lian , Tian Ye , Wei Qilin
Published: January 2024

As derivatives of metal halide perovskite materials, low-dimensional metal halide materials have become important materials that have attracted much attention in recent years. As one branch, zinc-based metal halides have the potential for practical applications due to their lead-free, low-toxicity and high-stability characteristics. However, pure zinc-based metal halide materials are still limited by their poor optical properties and cannot achieve large-scale practical applications. Therefore, in this work, we report an organic–inorganic hybrid zero-dimensional zinc bromide, (TDMP)ZnBr4, using transition metal Mn2+ ions as dopants and incorporating them into the (TDMP)ZnBr4 lattice. The original non-emissive (TDMP)ZnBr4 exhibits bright green emission under the excitation of external UV light after the introduction of Mn2+ ions with a PL peak position located at 538 nm and a PLQY of up to 91.2%. Through the characterization of relevant photophysical properties and the results of theoretical calculations, we confirm that this green emission in Mn2+:(TDMP)ZnBr4 originates from the 4T1 → 6A1 optical transition process of Mn2+ ions in the lattice structure, and the near-unity PLQY benefits from highly localized electrons generated by the unique zero-dimensional structure of the host material (TDMP)ZnBr4. This work provides theoretical guidance and reference for expanding the family of zinc-based metal halide materials and improving and controlling their optical properties through ion doping.
Modeling of the Flow Field and Clad Geometry of a Molten Pool during Laser Cladding of CoCrCuFeNi High-Entropy Alloys
Authors: Tian Dachuan , Li Chonggui , Hu Zhiguo , Li Xintong , Guo Yajun , Feng Xiaosong , Xu Zhenhai , Sun Xiaoguang , Li Wenge
Published: January 2024

A flow field analysis was performed in this research using the ANSYS Fluent module, and a dynamic heat source employing UDF was constructed using the DEFINE_PROFILE macro. A VOF model was developed to track the volume fraction of each fluid throughout the computational domain as well as the steady-state or transient condition of the liquid–gas interface in the free liquid surface area. To determine the distribution state and regularity of the molten pool flow field, the flow field velocity was calculated iteratively by linking the Simple algorithm with the horizontal set method. The molten pool was concave, indicating that the key hole was distributed narrowly. Inserting cross-sections at different depths yielded the vector distribution of the molten pool flow velocity along the depth direction. We set up monitoring sites along the molten pool’s depth direction and watched the flow change over time. We investigated the effects of the process parameters on the flow field’s vector distribution.
Renewable Carbonaceous Materials from Biomass in Catalytic Processes: A Review
Authors: Villora-Picó Juan J. , González-Arias Judith , Baena-Moreno Francisco M. , Reina Tomás R.
Published: January 2024

This review paper delves into the diverse ways in which carbonaceous resources, sourced from renewable and sustainable origins, can be used in catalytic processes. Renewable carbonaceous materials that come from biomass-derived and waste feedstocks are key to developing more sustainable processes by replacing traditional carbon-based materials. By examining the potential of these renewable carbonaceous materials, this review aims to shed light on their significance in fostering environmentally conscious and sustainable practices within the realm of catalysis. The more important applications identified are biofuel production, tar removal, chemical production, photocatalytic systems, microbial fuel cell electrodes, and oxidation applications. Regarding biofuel production, biochar-supported catalysts have proved to be able to achieve biodiesel production with yields exceeding 70%. Furthermore, hydrochars and activated carbons derived from diverse biomass sources have demonstrated significant tar removal efficiency. For instance, rice husk char exhibited an increased BET surface area from 2.2 m2/g to 141 m2/g after pyrolysis at 600 °C, showcasing its effectiveness in adsorbing phenol and light aromatic hydrocarbons. Concerning chemical production and the oxidation of alcohols, the influence of biochar quantity and pre-calcination temperature on catalytic performance has been proven, achieving selectivity toward benzaldehyde exceeding 70%.
Insight into the Growth Mechanism of Low-Temperature Synthesis of High-Purity Lithium Slag-Based Zeolite A
Authors: Li Li , Xu Shicheng , Liu Ze , Wang Dongmin
Published: January 2024

The utilization of lithium slag (LS), a solid waste generated during the production of lithium carbonate, poses challenges due to its high sulfur content. This study presents a novel approach to enhancing the value of LS by employing alkali fusion and hydrothermal synthesis techniques to produce zeolite A at low temperatures. The synthesis of high-purity and crystalline lithium-slag-based zeolite A (LSZ) at 60 °C is reported for the first time in this research. The phase, morphology, particle size, and structure of LSZ were characterized by XRD, SEM, TEM, N2 adsorption, and UV Raman spectroscopy, respectively. High-purity and crystalline zeolite A was successfully obtained under hydrothermal conditions of 60 °C, an NaOH concentration of 2.0 mol/L, and a hydrothermal time of 8 h. The samples synthesized at 60 °C exhibited better controllability and almost no byproduct of sodalite occurred compared to zeolite A synthesized at room temperature or conventional temperature (approximately 90 °C). Additionally, the growth mechanism of LSZ was elucidated, challenging the traditional understanding of utilization of lithium and enabling the synthesis of various zeolites at lower temperatures.