bluuebunny/crossref_metadata_2025 huggingface dataset:
Estimation of the Short-Term Impact of Climate-Change-Related Factors on Wood Supply in Poland in 2023–2025
Authors: Kotlarz Jan , Bejger Sylwester
Published: January 2024

In this study, we analyzed in situ data from the years 2018–2022 encompassing entire forest plantations in Poland. Based on data regarding stand density and the occurrence of fungal, water-related, climate-related, fire, and insect factors that may intensify with climate changes, we determined the correlation between their occurrence and the decline in wood increments for six tree species: pine, birch, oak, spruce, beech, and alder. Subsequently, we identified age intervals in which the species–factor interaction exhibited statistically significant effects. Next, we developed neural network models for short-term wood increment predictions. Utilizing these models, we estimated a reduction in wood supply harvested in accordance with the plans for the years 2023–2025 assuming a tenfold greater intensity of factors than in 2022. Findings indicate: birch: water-related factors may reduce wood production by 0.1%–0.2%. This aligns with previous research linking drought to birch wood decline, highlighting its sensitivity to water-related issues. Oak: fungal and insect factors could decrease wood production by up to 0.1%. Prior studies emphasize the significant influence of fungal diseases on oak health and regeneration, as well as the impact of insect infestation on wood production. Alder: water-related factors may lead to a slight reduction in wood production, approximately 0.02%. The impact is significant within specific age ranges, indicating potential effects on harvesting. Pine: water- and climate-related factors may result in up to a 0.05% reduction in wood production. Pine, a key forest-forming species in Poland, is notably sensitive to these factors, especially as it nears harvesting age. Spruce: insects, fungi, and climate-related factors could lead to a reduction in wood production of up to 0.2%–0.3%. Analyses demonstrate sensitivity, resulting in a noticeable growth differential compared to the typical rate. Short-term predictions based on neural networks were developed, acknowledging their suitability for short-term forecasts due to uncertainties regarding long-term factor impacts. Additionally, our study discussed modeling wood increments in divisions well below the harvesting time, emphasizing that the influence of current and 2023–2025 factors on wood increments and supply may only manifest several decades from now. These results imply important indications for the economic and financial performance of the wood industry.
Correction: Bopape et al. The Genome of a Pigeonpea Compatible Rhizobial Strain ‘10ap3’ Appears to Lack Common Nodulation Genes. Genes 2023, 14, 1084
Authors: Bopape Francina L. , Hassen Ahmed Idris , Chiulele Rogerio M. , Shonhai Addmore , Gwata Eastonce T.
Published: January 2024

Prof. Dr. Ahmed Idris Hassen was not included as an author in the original publication [...]
Women, Addictions, Mental Health, Dishonesty, and Crime Stigma: Solutions to Reduce the Social Harms of Stigma
Authors: Page Sarah , Fedorowicz Sophia , McCormack Fiona , Whitehead Stephen
Published: January 2024

British drug policies could underserve women with treatment needs, and this paper provides evidence that communication through the words and actions of professionals across drug and alcohol services, health and mental health, social work and the criminal justice sector can leave women feeling stigmatised and failed. Women live with the stigma of ‘the lying addict’; however, documents and courtroom statements provided by professionals can misrepresent women’s experiences, which exacerbates social harm. Data are drawn from feminist participatory action research, where female lived experience experts worked alongside academics to implement a qualitative study using interviews and focus groups with women using treatment services (n = 28) and an online world café with professionals working with these women (n = 9) and further professionals providing support at lived experience data collection events (n = 5). This data set is cross-referenced with one-to-one and small-group interviews with professionals in the field (n = 17) conducted by a third-sector partner. Findings establish that stigma negatively impacts the identification of treatment needs and access to timely and appropriate service delivery. Social harms to women with addictions could be significantly reduced with timely, authentic, honest, gender-informed and trauma-informed practices for girls and women using drugs and alcohol to self-medicate from traumatic experiences.
Semantic Segmentation and Roof Reconstruction of Urban Buildings Based on LiDAR Point Clouds
Authors: Sun Xiaokai , Guo Baoyun , Li Cailin , Sun Na , Wang Yue , Yao Yukai
Published: January 2024

In urban point cloud scenarios, due to the diversity of different feature types, it becomes a primary challenge to effectively obtain point clouds of building categories from urban point clouds. Therefore, this paper proposes the Enhanced Local Feature Aggregation Semantic Segmentation Network (ELFA-RandLA-Net) based on RandLA-Net, which enables ELFA-RandLA-Net to perceive local details more efficiently by learning geometric and semantic features of urban feature point clouds to achieve end-to-end building category point cloud acquisition. Then, after extracting a single building using clustering, this paper utilizes the RANSAC algorithm to segment the single building point cloud into planes and automatically identifies the roof point cloud planes according to the point cloud cloth simulation filtering principle. Finally, to solve the problem of building roof reconstruction failure due to the lack of roof vertical plane data, we introduce the roof vertical plane inference method to ensure the accuracy of roof topology reconstruction. The experiments on semantic segmentation and building reconstruction of Dublin data show that the IoU value of semantic segmentation of buildings for the ELFA-RandLA-Net network is improved by 9.11% compared to RandLA-Net. Meanwhile, the proposed building reconstruction method outperforms the classical PolyFit method.
Specific Circular RNA Signature of Endothelial Cells: Potential Implications in Vascular Pathophysiology
Authors: Diallo Leïla Halidou , Mariette Jérôme , Laugero Nathalie , Touriol Christian , Morfoisse Florent , Prats Anne-Catherine , Garmy-Susini Barbara , Lacazette Eric
Published: January 2024

Circular RNAs (circRNAs) are a recently characterized family of gene transcripts forming a covalently closed loop of single-stranded RNA. The extent of their potential for fine-tuning gene expression is still being discovered. Several studies have implicated certain circular RNAs in pathophysiological processes within vascular endothelial cells and cancer cells independently. However, to date, no comparative study of circular RNA expression in different types of endothelial cells has been performed and analysed through the lens of their central role in vascular physiology and pathology. In this work, we analysed publicly available and original RNA sequencing datasets from arterial, veinous, and lymphatic endothelial cells to identify common and distinct circRNA expression profiles. We identified 4713 distinct circRNAs in the compared endothelial cell types, 95% of which originated from exons. Interestingly, the results show that the expression profile of circular RNAs is much more specific to each cell type than linear RNAs, and therefore appears to be more suitable for distinguishing between them. As a result, we have discovered a specific circRNA signature for each given endothelial cell type. Furthermore, we identified a specific endothelial cell circRNA signature that is composed four circRNAs: circCARD6, circPLXNA2, circCASC15 and circEPHB4. These circular RNAs are produced by genes that are related to endothelial cell migration pathways and cancer progression. More detailed studies of their functions could lead to a better understanding of the mechanisms involved in physiological and pathological (lymph)angiogenesis and might open new ways to tackle tumour spread through the vascular system.
Secretome Analyses Identify FKBP4 as a GBA1-Associated Protein in CSF and iPS Cells from Parkinson’s Disease Patients with GBA1 Mutations
Authors: Kojima Rika , Paslawski Wojciech , Lyu Guochang , Arenas Ernest , Zhang Xiaoqun , Svenningsson Per
Published: January 2024

Mutations in the GBA1 gene increase the risk of developing Parkinson’s disease (PD). However, most carriers of GBA1 mutations do not develop PD throughout their lives. The mechanisms of how GBA1 mutations contribute to PD pathogenesis remain unclear. Cerebrospinal fluid (CSF) is used for detecting pathological conditions of diseases, providing insights into the molecular mechanisms underlying neurodegenerative disorders. In this study, we utilized the proximity extension assay to examine the levels of metabolism-linked protein in the CSF from 17 PD patients carrying GBA1 mutations (GBA1-PD) and 17 idiopathic PD (iPD). The analysis of CSF secretome in GBA1-PD identified 11 significantly altered proteins, namely FKBP4, THOP1, GLRX, TXNDC5, GAL, SEMA3F, CRKL, APLP1, LRP11, CD164, and NPTXR. To investigate GBA1-associated CSF changes attributed to specific neuronal subtypes responsible for PD, we analyzed the cell culture supernatant from GBA1-PD-induced pluripotent stem cell (iPSC)-derived midbrain dopaminergic (mDA) neurons. The secretome analysis of GBA1-PD iPSC-derived mDA neurons revealed that five differently regulated proteins overlapped with those identified in the CSF analysis: FKBP4, THOP1, GLRX, GAL, and CRKL. Reduced intracellular level of the top hit, FKPB4, was confirmed via Western Blot. In conclusion, our findings identify significantly altered CSF GBA1-PD-associated proteins with FKPB4 being firmly attributed to mDA neurons.
A Molecular Dynamics Simulation of Complexes of Fullerenes and Lysine-Based Peptide Dendrimers with and without Glycine Spacers
Authors: Bezrodnyi Valeriy V. , Mikhtaniuk Sofia E. , Shavykin Oleg V. , Sheveleva Nadezhda N. , Markelov Denis A. , Neelov Igor M.
Published: January 2024

The development of new nanocontainers for hydrophobic drugs is one of the most important tasks of drug delivery. Dendrimers with hydrophobic interiors and soluble terminal groups have already been used as drug carriers. However, the most convenient candidates for this purpose are peptide dendrimers since their interiors could be modified by hydrophobic amino acid residues with a greater affinity for the transported molecules. The goal of this work is to perform the first molecular dynamics study of the complex formation of fullerenes C60 and C70 with Lys-2Gly, Lys G2, and Lys G3 peptide dendrimers in water. We carried out such simulations for six different systems and demonstrated that both fullerenes penetrate all these dendrimers and form stable complexes with them. The density and hydrophobicity inside the complex are greater than in dendrimers without fullerene, especially for complexes with Lys-2Gly dendrimers. It makes the internal regions of complexes less accessible to water and counterions and increases electrostatic and zeta potential compared to single dendrimers. The results for complexes based on Lys G2 and Lys G3 dendrimers are similar but less pronounced. Thus, all considered peptide dendrimers and especially the Lys-2Gly dendrimer could be used as nanocontainers for the delivery of fullerenes.
Cinnamyl Alcohol Attenuates Adipogenesis in 3T3-L1 Cells by Arresting the Cell Cycle
Authors: Choi Yae Rim , Kim Young-Suk , Kim Min Jung
Published: January 2024

Cinnamyl alcohol (CA) is an aromatic compound found in several plant-based resources and has been shown to exert anti-inflammatory and anti-microbial activities. However, the anti-adipogenic mechanism of CA has not been sufficiently studied. The present study aimed to investigate the effect and mechanism of CA on the regulation of adipogenesis. As evidenced by Oil Red O staining, Western blotting, and real-time PCR (RT-PCR) analyses, CA treatment (6.25–25 μM) for 8 d significantly inhibited lipid accumulation in a concentration-dependent manner and downregulated adipogenesis-related markers (peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer-binding protein α (C/EBPα), fatty acid binding protein 4 (FABP4), adiponectin, fatty acid synthase (FAS)) in 3-isobutyl-1-methylxanthine, dexamethasone, and insulin(MDI)-treated 3T3-L1 adipocytes. In particular, among the various differentiation stages, the early stage of adipogenesis was critical for the inhibitory effect of CA. Cell cycle analysis using flow cytometry and Western blotting showed that CA effectively inhibited MDI-induced initiation of mitotic clonal expansion (MCE) by arresting the cell cycle in the G0/G1 phase and downregulating the expression of C/EBPβ, C/EBPδ, and cell cycle markers (cyclin D1, cyclin-dependent kinase 6 (CDK6), cyclin E1, CDK2, and cyclin B1). Moreover, AMP-activated protein kinase α (AMPKα), acetyl-CoA carboxylase (ACC), and extracellular signal-regulated kinase 1/2 (ERK1/2), markers of upstream signaling pathways, were phosphorylated during MCE by CA. In conclusion, CA can act as an anti-adipogenic agent by inhibiting the AMPKα and ERK1/2 signaling pathways and the cell cycle and may also act as a potential therapeutic agent for obesity.
Alkaline Phosphatase Relieves Colitis in Obese Mice Subjected to Forced Exercise via Its Anti-Inflammatory and Intestinal Microbiota-Shaping Properties
Authors: Wojcik-Grzybek Dagmara , Sliwowski Zbigniew , Kwiecien Slawomir , Ginter Grzegorz , Surmiak Marcin , Hubalewska-Mazgaj Magdalena , Chmura Anna , Wojcik Adrianna , Kosciolek Tomasz , Danielak Aleksandra , Targosz Aneta , Strzalka Malgorzata , Szczyrk Urszula , Ptak-Belowska Agata , Magierowski Marcin , Bilski Jan , Brzozowski Tomasz
Published: January 2024

Intestinal alkaline phosphatase (IAP) is an enzyme that plays a protective role in the gut. This study investigated the effect of IAP treatment on experimental colitis in mice subjected to forced exercise on a high-fat diet. C57BL/6 mice with TNBS colitis were fed a high-fat diet and subjected to forced treadmill exercise with or without IAP treatment. Disease activity, oxidative stress, inflammatory cytokines, and gut microbiota were assessed. Forced exercise exacerbated colitis in obese mice, as evidenced by increased disease activity index (DAI), oxidative stress markers, and proinflammatory adipokines and cytokines. IAP treatment significantly reduced these effects and promoted the expression of barrier proteins in the colonic mucosa. Additionally, IAP treatment altered the gut microbiota composition, favoring beneficial Verrucomicrobiota and reducing pathogenic Clostridia and Odoribacter. IAP treatment ameliorates the worsening effect of forced exercise on murine colitis by attenuating oxidative stress, downregulating proinflammatory biomarkers, and modulating the gut microbiota. IAP warrants further investigation as a potential therapeutic strategy for ulcerative colitis.
Effects of Endohedral Gd-Containing Fullerenols with a Different Number of Oxygen Substituents on Bacterial Bioluminescence
Authors: Stepin Evsei A. , Sushko Ekaterina S. , Vnukova Natalia G. , Churilov Grigoriy N. , Rogova Anastasia V. , Tomilin Felix N. , Kudryasheva Nadezhda S.
Published: January 2024

Gadolinium (Gd)-containing fullerenols are perspective agents for magnetic resonance imaging and cancer research. They combine the unique paramagnetic properties of Gd with solubility in water, low toxicity and antiradical activity of fullerenols. We compared the bioeffects of two Gd-containing fullerenols with a different number of oxygen groups—20 and 42: Gd@C82O20H14 and Gd@C82O42H32. The bioluminescent bacteria-based assay was applied to monitor the toxicity of fullerenols, bioluminescence was applied as a signal physiological parameter, and bacterial enzyme-based assay was used to evaluate the fullerenol effects on enzymatic intracellular processes. Chemiluminescence luminol assay was applied to monitor the content of reactive oxygen species (ROS) in bacterial and enzymatic media. It was shown that Gd@C82O42H32 and Gd@C82O20H14 inhibited bacterial bioluminescence at >10−1 and >10−2 gL−1, respectively, revealing a lower toxicity of Gd@C82O42H32. Low-concentration (10−3–10−1 gL−1) bacterial bioluminescence activation by Gd@C82O42H32 was observed, while this activation was not found under exposure to Gd@C82O20H14. Additional carboxyl groups in the structure of Gd@C82O42H32 were determined by infrared spectroscopy and confirmed by quantum chemical calculations. The groups were supposed to endow Gd@C82O42H32 with higher penetration ability through the cellular membrane, activation ability, lower toxicity, balancing of the ROS content in the bacterial suspensions, and lower aggregation in aqueous media.
Serum Uric Acid Associates with Systemic Complement C3 Activation in Severe ANCA-Associated Renal Vasculitides
Authors: Baier Eva , Kluge Ingmar Alexander , Hakroush Samy , Korsten Peter , Tampe Björn
Published: January 2024

Involvement of the complement system is key to the pathogenesis of antineutrophil cytoplasmic antibody (ANCA)-associated renal vasculitis, but immunometabolic implications, especially on serum uric acid (UA) levels, still need to be elucidated. A total of 34 patients with biopsy-proven ANCA-associated renal vasculitis between 2015 and 2020 were retrospectively enrolled. Serum UA levels were correlated with clinical and histopathological characteristics, separated for critically ill (CI, n = 19), myeloperoxidase (MPO)-ANCA (n = 21) and proteinase 3 (PR3)-ANCA (n = 13) subgroups. We here identified inverse correlations of serum UA levels and complement C3 levels in the total cohort (p = 0.005) and the CI subgroup (p < 0.001). Intrarenal complement C4d deposition in venules correlated with serum UA levels in the total cohort (p = 0.007) and in the CI subgroup (p = 0.016). Significant associations of serum UA levels and tubulitis in areas of scarred cortex (t-IFTA) were identified in the total cohort (p = 0.008), and both subgroups of CI (p = 0.034) and MPO-ANCA (p = 0.029). In PR3-ANCA, interstitial fibrosis (ci) was observed as the strongest association with serum UA levels (p = 0.022). Our observations broaden our current understanding of contributory metabolic factors that influence the initial disease course in ANCA-associated renal vasculitis.
Proline Dehydrogenase (PRODH) Is Expressed in Lung Adenocarcinoma and Modulates Cell Survival and 3D Growth by Inducing Cellular Senescence
Authors: Grossi Sarah , Berno Elena , Chiofalo Priscilla , Chiaravalli Anna Maria , Cinquetti Raffaella , Bruno Antonino , Palano Maria Teresa , Gallazzi Matteo , La Rosa Stefano , Sessa Fausto , Acquati Francesco , Campomenosi Paola
Published: January 2024

The identification of markers for early diagnosis, prognosis, and improvement of therapeutic options represents an unmet clinical need to increase survival in Non-Small Cell Lung Cancer (NSCLC), a neoplasm still characterized by very high incidence and mortality. Here, we investigated whether proline dehydrogenase (PRODH), a mitochondrial flavoenzyme catalyzing the key step in proline degradation, played a role in NSCLC tumorigenesis. PRODH expression was investigated by immunohistochemistry; digital PCR, quantitative PCR, immunoblotting, measurement of reactive oxygen species (ROS), and functional cellular assays were carried out. PRODH expression was found in the majority of lung adenocarcinomas (ADCs). Patients with PRODH-positive tumors had better cancer-free specific and overall survival compared to those with negative tumors. Ectopic modulation of PRODH expression in NCI-H1299 and the other tested lung ADC cell lines decreased cell survival. Moreover, cell proliferation curves showed delayed growth in NCI-H1299, Calu-6 and A549 cell lines when PRODH-expressing clones were compared to control clones. The 3D growth in soft agar was also impaired in the presence of PRODH. PRODH increased reactive oxygen species production and induced cellular senescence in the NCI-H1299 cell line. This study supports a role of PRODH in decreasing survival and growth of lung ADC cells by inducing cellular senescence.
The Role of TRPM7 in Oncogenesis
Authors: Köles László , Ribiczey Polett , Szebeni Andrea , Kádár Kristóf , Zelles Tibor , Zsembery Ákos
Published: January 2024

This review summarizes the current understanding of the role of transient receptor potential melastatin-subfamily member 7 (TRPM7) channels in the pathophysiology of neoplastic diseases. The TRPM family represents the largest and most diverse group in the TRP superfamily. Its subtypes are expressed in virtually all human organs playing a central role in (patho)physiological events. The TRPM7 protein (along with TRPM2 and TRPM6) is unique in that it has kinase activity in addition to the channel function. Numerous studies demonstrate the role of TRPM7 chanzyme in tumorigenesis and in other tumor hallmarks such as proliferation, migration, invasion and metastasis. Here we provide an up-to-date overview about the possible role of TRMP7 in a broad range of malignancies such as tumors of the nervous system, head and neck cancers, malignant neoplasms of the upper gastrointestinal tract, colorectal carcinoma, lung cancer, neoplasms of the urinary system, breast cancer, malignant tumors of the female reproductive organs, prostate cancer and other neoplastic pathologies. Experimental data show that the increased expression and/or function of TRPM7 are observed in most malignant tumor types. Thus, TRPM7 chanzyme may be a promising target in tumor therapy.
DNA Mutagenicity of Hydroxyhydroquinone in Roasted Coffee Products and Its Suppression by Chlorogenic Acid, a Coffee Polyphenol, in Oxidative-Damage-Sensitive SAMP8 Mice
Authors: Unno Keiko , Taguchi Kyoko , Hase Tadashi , Meguro Shinichi , Nakamura Yoriyuki
Published: January 2024

Hydroxyhydroquinone (HHQ) is an oxidative component produced by roasting coffee beans and has been reported to generate relatively large amounts of reactive oxygen species (ROS). In this study, we used senescence-accelerated mouse prone 8 (SAMP8) mice to determine whether HHQ consumption increases oxidative-stress-induced injury, because in SAMP8 mice, the activity of 8-oxoguanine DNA glycosylase 1, which repairs oxidative modifications in DNA, is decreased. The results showed that two out of twelve (16.7%) HHQ-treated mice presented polyuria and glucosuria around 2 months after the start of treatment, indicating that HHQ may act as a mutagen against SAMP8 mice, which is sensitive to oxidative damage. No abnormalities were observed in the chlorogenic acid (coffee polyphenol, CPP)-treated group. The concentration of hydrogen peroxide in the serum of SAMP8 mice was significantly higher than that in SAMR1 (senescence-resistant) control mice, and the concentration was further increased in the HHQ-treated group. CPP, when coexisting with HHQ at the rate contained in roasted coffee, decreased the amount of hydrogen peroxide in the serum of SAMP8 mice. Although CPP can act both oxidatively and antioxidatively as a polyphenol, CPP acts more antioxidatively when coexisting with HHQ. Thus, the oxidative effect of HHQ was shown to be counteracted by CPP.
Recombinant TP-84 Bacteriophage Glycosylase–Depolymerase Confers Activity against Thermostable Geobacillus stearothermophilus via Capsule Degradation
Authors: Łubkowska Beata , Sobolewski Ireneusz , Adamowicz Katarzyna , Zylicz-Stachula Agnieszka , Skowron Piotr M.
Published: January 2024

The TP-84 bacteriophage, which infects Geobacillus stearothermophilus strain 10 (G. stearothermophilus), has a genome size of 47.7 kilobase pairs (kbps) and contains 81 predicted protein-coding ORFs. One of these, TP84_26 encodes a putative tail fiber protein possessing capsule depolymerase activity. In this study, we cloned the TP84_26 gene into a high-expression Escherichia coli (E. coli) system, modified its N-terminus with His-tag, expressed both the wild type gene and His-tagged variant, purified the recombinant depolymerase variants, and further evaluated their properties. We developed a direct enzymatic assay for the depolymerase activity toward G. stearothermophilus capsules. The recombinant TP84_26 protein variants effectively degraded the existing bacterial capsules and inhibited the formation of new ones. Our results provide insights into the novel TP84_26 depolymerase with specific activity against thermostable G. stearothermophilus and its role in the TP-84 life cycle. The identification and characterization of novel depolymerases, such as TP84_26, hold promise for innovative strategies to combat bacterial infections and improve various industrial processes.
p21 as a Predictor and Prognostic Indicator of Clinical Outcome in Rectal Cancer Patients
Authors: Ooi Li Ching , Ho Vincent , Zhu Jing Zhou , Lim Stephanie , Chung Liping , Abubakar Askar , Rutland Tristan , Chua Wei , Ng Weng , Lee Mark , Morgan Matthew , MacKenzie Scott , Lee Cheok Soon
Published: January 2024

The cell cycle plays a key and complex role in the development of human cancers. p21 is a potent cyclin-dependent kinase inhibitor (CDKI) involved in the promotion of cell cycle arrest and the regulation of cellular senescence. Altered p21 expression in rectal cancer cells may affect tumor cells’ behavior and resistance to neoadjuvant and adjuvant therapy. Our study aimed to ascertain the relationship between the differential expression of p21 in rectal cancer and patient survival outcomes. Using tissue microarrays, 266 rectal cancer specimens were immunohistochemically stained for p21. The expression patterns were scored separately in cancer cells retrieved from the center and the periphery of the tumor; compared with clinicopathological data, tumor regression grade (TRG), disease-free, and overall survival. Negative p21 expression in tumor periphery cells was significantly associated with longer overall survival upon the univariate (p = 0.001) and multivariable analysis (p = 0.003, HR = 2.068). Negative p21 expression in tumor periphery cells was also associated with longer disease-free survival in the multivariable analysis (p = 0.040, HR = 1.769). Longer overall survival times also correlated with lower tumor grades (p= 0.011), the absence of vascular and perineural invasion (p = 0.001; p < 0.005), the absence of metastases (p < 0.005), and adjuvant treatment (p = 0.009). p21 expression is a potential predictive and prognostic biomarker for clinical outcomes in rectal cancer patients. Negative p21 expression in tumor periphery cells demonstrated significant association with longer overall survival and disease-free survival. Larger prospective studies are warranted to investigate the ability of p21 to identify rectal cancer patients who will benefit from neoadjuvant and adjuvant therapy.
Mechanism of Iron Ion Homeostasis in Intestinal Immunity and Gut Microbiota Remodeling
Authors: Bao Honghong , Wang Yi , Xiong Hanlin , Xia Yaoyao , Cui Zhifu , Liu Lingbin
Published: January 2024

Iron is a vital trace element that plays an important role in humans and other organisms. It plays an active role in the growth, development, and reproduction of bacteria, such as Bifidobacteria. Iron deficiency or excess can negatively affect bacterial hosts. Studies have reported a major role of iron in the human intestine, which is necessary for maintaining body homeostasis and intestinal barrier function. Organisms can maintain their normal activities and regulate some cancer cells in the body by regulating iron excretion and iron-dependent ferroptosis. In addition, iron can modify the interaction between hosts and microorganisms by altering their growth and virulence or by affecting the immune system of the host. Lactic acid bacteria such as Lactobacillus acidophilus (L. acidophilus), Lactobacillus rhamnosus (L. rhamnosus), and Lactobacillus casei (L. casei) were reported to increase trace elements, protect the host intestinal barrier, mitigate intestinal inflammation, and regulate immune function. This review article focuses on the two aspects of the iron and gut and generally summarizes the mechanistic role of iron ions in intestinal immunity and the remodeling of gut microbiota.
Alterations in Histone Methylation States Increased Profusion of Lethal(2)-Essential-for-Life-Like (l(2)elf), Trithorax and Polycomb Genes in Apis mellifera under Heat Stress
Authors: Alghamdi Ahmad A. , Alattal Yehya Z.
Published: January 2024

Histone post-translational modifications (PTMs) represent a key mechanism in the thermal adaptation of the honeybee Apis mellifera. In this study, a chromatin immunoprecipitation assay and qPCR were employed to explore the changes in the methylation states of H3K4m2, H3K4m3, H3K27m2 and H3K27m3 associated with l2efl (ID: 72474, 724405, 724488), histone methyltransferases (HMTs) ((trx) and PR-set7) and Polycomb (Pc) and (Su(z)12) genes in A. m. jemenitica (tolerant subspecies) and A. m. carnica (susceptible subspecies) in response to heat treatment (42 °C for 1 h). The results revealed significant enrichment fold changes in the methylation/demethylation of most H3K4 and H3K27 marks at all targeted genes. These changes increased the profusion of l2efl (ID: 72474, 724405, 724488), histone methyltransferases (HMTs) (trx) and Polycomb (Pc) and Su(z)12 and decreased the profusion of HMT (PR-set7) in both honeybee subspecies. The changes in the methylation enrichment folds of histone methyltransferases (HMTs) ((trx), PR-set) and Polycomb (Pc), Su(z)12 genes demonstrate the well-harmonized coordination of epigenetic gene regulation in response to heat treatment. Compared to the control, the changes in the methylation enrichment folds of H3K4m3 at Polycomb Su(z)12 were about 30× and 100× higher in treated A. m. jemenitica and A.m. carnica, respectively. Similarly, changes in the methylation/demethylation enrichment folds of HMT (trx) and Polycomb (Pc) and Su(z)12 were 2–3× higher in A. m. carnica than in A. m. jemenitica after treatment (42 °C). It is evident that post-translational chromatin modification in both honeybee subspecies can diminish heat stress impact by (I) increasing the transcriptional provision of l2efl associated with survival and (II) increasing the silencing of genes associated with general cellular activities.
Can Consultation by a Clinical Pharmacist Prevent Morbidity and Mortality in Patients Undergoing Bariatric Surgery?
Authors: Azran Carmil , Dahan Almog Eliyahu , Shimoni Orly , Dicker Dror , Hammerman Ariel , Dahan Arik
Published: January 2024

The purpose of this work was to investigate the effect of clinical pharmacist consultation on the long-term morbidity and mortality outcomes among patients undergoing bariatric surgery. In this retrospective cohort study, 165 bariatric patients at Herzliya Medical Center who were identified as complex cases and were consulted by a clinical pharmacist (2013–2019) were compared with a wider group of bariatric patients with chronic diseases who were recorded in the Israeli General Bariatric Registry during the same years. The primary outcomes were rates of surgical complications, re-hospitalizations, and death up to one year after surgery. The secondary outcome was the rate of re-hospitalizations in different time periods. The twelve (12)-month rate of re-hospitalization in the intervention group was 10.9% vs. 19.5% in the comparison group (p = 0.005); the rate of documented postoperative complications was 2.7% vs. 3.9% (p = 0.462), and mortality was null vs. 0.16%, respectively. As for the secondary outcomes, the rates of re-hospitalizations in the periods of 0–30, 31–90, 91–180, and 181–365 days after surgery were 1.8% vs. 5.3% (p = 0.046), 2.4% vs. 4.1% (p = 0.278), 3.6% vs. 4.8% (p = 0.476), and 7.3% vs. 9.9% (p = 0.256) in the intervention vs. comparison cohorts, respectively. In conclusion, this study demonstrates the importance and benefit of referring to a specialized clinical pharmacist around bariatric surgery for improving patient safety, especially in complex patients. This is the first study to look at the long-term effects of clinical pharmacist consultation on re-hospitalization and mortality among bariatric patients, and our encouraging outcomes should hopefully stimulate more studies to show the invaluable role of specialized clinical pharmacists.
ChatGPT May Offer an Adequate Substitute for Informed Consent to Patients Prior to Total Knee Arthroplasty—Yet Caution Is Needed
Authors: Kienzle Arne , Niemann Marcel , Meller Sebastian , Gwinner Clemens
Published: January 2024

Prior to undergoing total knee arthroplasty (TKA), surgeons are often confronted with patients with numerous questions regarding the procedure and the recovery process. Due to limited staff resources and mounting individual workload, increased efficiency, e.g., using artificial intelligence (AI), is of increasing interest. We comprehensively evaluated ChatGPT’s orthopedic responses using the DISCERN instrument. Three independent orthopedic surgeons rated the responses across various criteria. We found consistently high scores, predominantly exceeding a score of three out of five in almost all categories, indicative of the quality and accuracy of the information provided. Notably, the AI demonstrated proficiency in conveying precise and reliable information on orthopedic topics. However, a notable observation pertains to the generation of non-existing references for certain claims. This study underscores the significance of critically evaluating references provided by ChatGPT and emphasizes the necessity of cross-referencing information from established sources. Overall, the findings contribute valuable insights into the performance of ChatGPT in delivering accurate orthopedic information for patients in clinical use while shedding light on areas warranting further refinement. Future iterations of natural language processing systems may be able to replace, in part or in entirety, the preoperative interactions, thereby optimizing the efficiency, accessibility, and standardization of patient communication.
Rethinking Fluid Responsiveness during Septic Shock: Ameliorate Accuracy of Noninvasive Cardiac Output Measurements through Evaluation of Arterial Biomechanical Properties
Authors: Papaioannou Vasileios , Papaioannou Theodoros
Published: January 2024

Beat-to-beat estimates of cardiac output from the direct measure of peripheral arterial blood pressure rely on the assumption that changes in the waveform morphology are related to changes in blood flow and vasomotor tone. However, in septic shock patients, profound changes in vascular tone occur that are not uniform across the entire arterial bed. In such cases, cardiac output estimates might be inaccurate, leading to unreliable evaluation of fluid responsiveness. Pulse wave velocity is the gold-standard method for assessing different arterial biomechanical properties. Such methods might be able to guide, personalize and optimize the management of septic patients.
Prognostic Value of Radiomic Analysis Using Pre- and Post-Treatment 18F-FDG-PET/CT in Patients with Laryngeal Cancer and Hypopharyngeal Cancer
Authors: Choi Joon Ho , Choi Joon Young , Woo Sang-Keun , Moon Ji Eun , Lim Chae Hong , Park Soo Bin , Seo Seongho , Ahn Yong Chan , Ahn Myung-Ju , Moon Seung Hwan , Park Jung Mi
Published: January 2024

Background: The prognostic value of conducting 18F-FDG PET/CT imaging has yielded different results in patients with laryngeal cancer and hypopharyngeal cancer, but these results are controversial, and there is a lack of dedicated studies on each type of cancer. This study aimed to evaluate whether combining radiomic analysis of pre- and post-treatment 18F-FDG PET/CT imaging features and clinical parameters has additional prognostic value in patients with laryngeal cancer and hypopharyngeal cancer. Methods: From 2008 to 2016, data on patients diagnosed with cancer of the larynx and hypopharynx were retrospectively collected. The patients underwent pre- and post-treatment 18F-FDG PET/CT imaging. The values of ΔPre-Post PET were measured from the texture features. Least absolute shrinkage and selection operator (LASSO) Cox regression was used to select the most predictive features to formulate a Rad-score for both progression-free survival (PFS) and overall survival (OS). Kaplan–Meier curve analysis and Cox regression were employed to assess PFS and OS. Then, the concordance index (C-index) and calibration plot were used to evaluate the performance of the radiomics nomogram. Results: Study data were collected for a total of 91 patients. The mean follow-up period was 71.5 mo. (8.4–147.3). The Rad-score was formulated based on the texture parameters and was significantly associated with both PFS (p = 0.024) and OS (p = 0.009). When predicting PFS, only the Rad-score demonstrated a significant association (HR 2.1509, 95% CI [1.100–4.207], p = 0.025). On the other hand, age (HR 1.116, 95% CI [1.041–1.197], p = 0.002) and Rad-score (HR 33.885, 95% CI [2.891–397.175], p = 0.005) exhibited associations with OS. The Rad-score value showed good discrimination when it was combined with clinical parameters in both PFS (C-index 0.802–0.889) and OS (C-index 0.860–0.958). The calibration plots also showed a good agreement between the observed and predicted survival probabilities. Conclusions: Combining clinical parameters with radiomics analysis of pre- and post-treatment 18F-FDG PET/CT parameters in patients with laryngeal cancer and hypopharyngeal cancer might have additional prognostic value.
Green Preparation and Functional Properties of Reinforced All-Cellulose Membranes Made from Corn Straw
Authors: Zhang Wentao , Wang Tianhao , Jiang Zeming , Gao Xin , Sun Changxia , Zhang Liping
Published: January 2024

In this study, all-cellulose nanocomposite (ACNC) was successfully prepared through a green and sustainable approach by using corn stalk as raw material, water as regeneration solvent, and recyclable two-component ionic liquid/DMSO as the solvent to dissolve cellulose. The morphology and structural properties of ACNC were determined by scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, and X-ray diffraction analysis, indicating homogeneity and good crystallinity. In addition, a comprehensive characterization of ACNC showed that CNF not only improved the thermal stability and mechanical characteristics of ACNC, but also significantly improved the oxygen barrier performance. The ACNC prepared in this work has a good appearance, smooth surface, and good optical transparency, which provides a potential application prospect for converting cellulose wastes such as corn straws into biodegradable packaging materials and electronic device encapsulation materials.
Critical Factors in Sample Collection and Preparation for Clinical Metabolomics of Underexplored Biological Specimens
Authors: de Souza Hygor M. R. , Pereira Tássia T. P. , de Sá Hanna C. , Alves Marina A. , Garrett Rafael , Canuto Gisele A. B.
Published: January 2024

This review article compiles critical pre-analytical factors for sample collection and extraction of eight uncommon or underexplored biological specimens (human breast milk, ocular fluids, sebum, seminal plasma, sweat, hair, saliva, and cerebrospinal fluid) under the perspective of clinical metabolomics. These samples are interesting for metabolomics studies as they reflect the status of living organisms and can be applied for diagnostic purposes and biomarker discovery. Pre-collection and collection procedures are critical, requiring protocols to be standardized to avoid contamination and bias. Such procedures must consider cleaning the collection area, sample stimulation, diet, and food and drug intake, among other factors that impact the lack of homogeneity of the sample group. Precipitation of proteins and removal of salts and cell debris are the most used sample preparation procedures. This review intends to provide a global view of the practical aspects that most impact results, serving as a starting point for the designing of metabolomic experiments.
Assessing the Activity of Eastern Himalayan Extensional Structures: Evidence from Low-Temperature Thermochronology of Granitic Rocks from Yadong
Authors: Xu Tiankun , Li Yalin , Stuart Finlay M. , Ma Zining , Bi Wenjun , Jia Yongyong , Yang Bo
Published: January 2024

The east–west-trending South Tibetan Detachment System (STDS) and north–south-trending rifts (NSTRs) are the two main types of extensional structures that have developed within the Tibetan Plateau during continent–continent collision since the early Cenozoic. They have played significant roles in the evolution of the plateau, but it is unclear how they are related genetically. In the Yadong area of the eastern Himalaya, the NSTRs cross-cut the STDS. Apatite and zircon fission track ages of a leucogranite pluton in the footwall of the two extensional faults can be used to reconstruct the cooling and exhumation history and thereby constrain the activity of extensional structures. The new AFT ages range from 10.96 ± 0.70 to 5.68 ± 0.37 Ma, and the ZFT age is 13.57 ± 0.61 Ma. Track length distributions are unimodal, albeit negatively skewed, with standard deviations between 1.4 and 2.1 µm and mean track lengths between 11.6 and 13.4 µm. In conjunction with previously published datasets, the thermal history of the region is best explained by three distinct pulses of exhumation in the last 16 Ma. The first pulse (16–12 Ma) records a brittle slip on the STDS. The two subsequent pulses are attributed to the movement on the Yadong normal fault. The normal fault initiated at ~12 Ma and experienced a pulse of accelerated exhumation between 6.2 and 4.7 Ma, probably reflecting the occurrence of two distinct phases of fault activity within the NSTRs, which were primarily instigated by slab tear of the subducting Indian plate.
Gravity Separation Tests of a Complex Rutile Ore
Authors: Wang Zhenxing , Zheng Yongxing , Huang Xiang , Wang Xiangding , Peng Jieli , Dai Zhe
Published: January 2024

The complex rutile ore containing TiO2 and ZrO2 exhibited a high economical value. To effectively recover TiO2 and ZrO2 from the raw sample, a complete gravity separation process including a spiral chute and a shaking table was proposed. Chemical constituents, phase, liberation degree and size distribution were firstly characterized by XRF, chemical analyses, XRD, EPMA-EDS and screening to understand the mineralogy. Then, two stages of spiral chute separation tests were performed to treat the complex rutile ore. A rough zircon concentrate containing 33.18% ZrO2 was obtained after the first-stage spiral chute and a rough rutile concentrate containing 56.77% TiO2 was obtained after the second-stage spiral chute. To further improve the grade and recovery of ZrO2 and TiO2 in the rough products obtained by spiral chutes, shaking table tests were performed. A zircon concentrate containing 42.65% ZrO2 and a rutile concentrate containing 61.75% TiO2 were obtained. For the tailing of the first-stage spiral chute, a rutile product assaying 57.50% TiO2 was obtained, and the tailing was directly discarded as waste after the shaking table tests. Moreover, the distribution regularities of ZrO2 and TiO2 in the products were further revealed by XRD analyses. Finally, a closed-circuit beneficiation process was proposed to treat the complex rutile ore for achieving comprehensive and effective utilization.
Whole-Rock Geochemistry and Mica Compositions in Lijiagou Pegmatite Spodumene Deposit, Western Sichuan, China
Authors: Chen Xiaojie , Chen Cuihua , Lai Xiang , Yang Yulong , Gu Ying , Cai Yunhua
Published: January 2024

The Lijiagou pegmatite spodumene deposit, located in the middle of the Songpan–Garze Fold Belt and southeast of the Ke’eryin ore field, is a newly discovered super-large deposit. In order to reveal the metallogenic tectonic environment and evolution process of pegmatite, based on the study of the geological characteristics of pegmatite, we carried out a whole-rock geochemical analysis of Ke’eryin two-mica granite and Lijiagou pegmatite and carried out a detailed electron probe microanalysis (EPMA) and laser ablation inductively coupled plasma mass spectrometry (LA-ICPMS) analysis of mica minerals in each zonal pegmatite. The results show that the Ke’eryin two-mica granite is mainly formed in the transition period from syn-collision to post-collision. After the end of the continental collision, the crust is squeezed and thickened in the post-collision extensional transition tectonic environment. Mica from the microcline pegmatite zone (MP) to the albite spodumene pegmatite zone (ASP) in pegmatite show different compositions and structural characteristics, with the evolution trend in the direction from muscovite to Li-bearing mica. The type of mica from MP to AP is mainly muscovite, and Li-bearing mica appears in ASP, which is secondary and metasomatic at the edge of primary muscovite. From MP to ASP, there was a negative correlation between Nb/Ta, K/Rb and the Li, Rb, and Cs contents of mica, while the contents of Li, Rb, Cs, and F in the Li-bearing mica of ASP increased sharply. This evidence illustrates that the favorable tectonic environment contributed to the formation of the Lijiagou pegmatitic spodumene deposit. Lijiagou pegmatite experienced the magmatic–hydrothermal evolution process and has a high degree of differentiation and evolution from MP to ASP, which gradually increased. Combined with the change in mica type, it is considered that ASP formed from the stage of magmatic transition to hydrothermal and was a hydrothermal environment, and Li, Rb, and Cs mainly began to enrich at the stage of magmatic–hydrothermal transition.
Targeted Delivery of Arctigenin Using Sialic Acid Conjugate-Modified Liposomes for the Treatment of Breast Cancer
Authors: Liu Shunfang , He Yaozhen , Feng Minding , Huang Yongtong , Wu Wenhao , Wang Jiu
Published: January 2024

Arctigenin (ATG) is a broad-spectrum antitumor drug with an excellent inhibitory effect on malignant tumors such as breast cancer, glioblastoma, liver cancer, and colon cancer. However, the clinical application of ATG is limited by its poor water solubility and quick hydrolysis in the liver, intestine, and plasma, which might hinder its application. Sialic acid (SA) recognizes selectin receptors overexpressed on the surface of tumor-associated macrophages. In this study, SA was conjugated with octadecylamine (ODA) to prepare SA-ODA, which was employed to prepare SA functionalized nanoliposomes (SA-Lip) to achieve breast cancer targeting. The formulations were finely optimized using the Box–Behnken design to achieve higher ATG loading. The size, ζ potential, entrapment efficiency, drug loading, and release behavior of ATG@SA-Lip were fully investigated in comparison with conventional ATG@Lip. The ATG@SA-Lip displayed more potent cytotoxicity and higher cellular internalization compared to ATG@Sol and ATG@Lip in both MCF7 and 4T1 cells. Notably, ATG@SA-Lip showed the lowest impact on the immune system. Our study demonstrates that SA-Lip has strong potential as a delivery system for the targeted delivery of ATG.