bluuebunny/crossref_metadata_2025 huggingface dataset:
Variant Aldehyde Dehydrogenase 2 (ALDH2*2) as a Risk Factor for Mechanical LA Substrate Formation and Atrial Fibrillation with Modest Alcohol Consumption in Ethnic Asians
Authors: Hung Chung-Lieh , Sung Kuo-Tzu , Chang Shun-Chuan , Liu Yen-Yu , Kuo Jen-Yuan , Huang Wen-Hung , Su Cheng-Huang , Liu Chuan-Chuan , Tsai Shin-Yi , Liu Chia-Yuan , Lee An-Sheng , Pan Szu-Hua , Wang Shih-Wei , Hou Charles Jia-Yin , Hung Ta-Chuan , Yeh Hung-I
Published: October 2021

Aldehyde dehydrogenase 2 (ALDH2) rs671 polymorphism is a common genetic variant in Asians that is responsible for defective toxic aldehyde and lipid peroxidation metabolism after alcohol consumption. The extent to which low alcohol consumption may cause atrial substrates to trigger atrial fibrillation (AF) development in users with ALDH2 variants remains to be determined. We prospectively enrolled 249 ethnic Asians, including 56 non-drinkers and 193 habitual drinkers (135 (70%) as ALDH2 wild-type: GG, rs671; 58 (30%) as ALDH2 variants: G/A or A/A, rs671). Novel left atrial (LA) mechanical substrates with dynamic characteristics were assessed using a speckle-tracking algorithm and correlated to daily alcohol consumption and ALDH2 genotypes. Despite modest and comparable alcohol consumption by the habitual alcohol users (14.3 [8.3~28.6] and 12.3 [6.3~30.7] g/day for those without and with ALDH2 polymorphism, p = 0.31), there was a substantial and graded increase in the 4-HNE adduct and prolonged PR, and a reduction in novel LA mechanical parameters (including peak atrial longitudinal strain (PALS) and phasic strain rates (reservoir, conduit, and booster pump functions), p < 0.05), rather than an LA emptying fraction (LAEF) or LA volume index across non-drinkers, and in habitual drinkers without and with ALDH2 polymorphism (all p < 0.05). The presence of ALDH2 polymorphism worsened the association between increasing daily alcohol dose and LAEF, PALS, and phasic reservoir and booster functions (all Pinteraction: <0.05). Binge drinking superimposed on regular alcohol use exclusively further worsened LA booster pump function compared to regular drinking without binge use (1.66 ± 0.57 vs. 1.97 ± 0.56 1/s, p = 0.001). Impaired LA booster function further independently helped to predict AF after consideration of the CHARGE-AF score (adjusted 1.68 (95% CI: 1.06–2.67), p = 0.028, per 1 z-score increment). Habitual modest alcohol consumption led to mechanical LA substrate formation in an ethnic Asian population, which was more pronounced in subjects harboring ALDH2 variants. Impaired LA booster functions may serve as a useful predictor of AF in such populations.
Nitric Oxide Mediation in Hydroxyurea and Nitric Oxide Metabolites’ Inhibition of Erythroid Progenitor Growth
Authors: Subotički Tijana , Mitrović Ajtić Olivera , Djikić Dragoslava , Kovačić Marijana , Santibanez Juan F. , Tošić Milica , Čokić Vladan P.
Published: October 2021

In several systems, hydroxyurea has been shown to trigger nitric oxide (NO) release or activation of NO synthase (NOS). To elucidate this duality in its pharmacological effects, during myelosuppression, we individually examined hydroxyurea’s (NO releasing agent) and NO metabolites’ (stable NO degradation products) effects on erythroid colony growth and NOS/NO levels in mice using NO scavenger 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (PTIO). Hydroxyurea and nitrite/nitrate decreased the bone marrow cellularity that was blocked by PTIO only for the NO metabolites. Hydroxyurea inhibition of colony-forming unit-erythroid (CFU-E) formation and reticulocytes was reversed by PTIO. Moreover, hydroxyurea, through a negative feedback mechanism, reduced inducible NOS (iNOS) expressing cells in CFU-E, also prevented by PTIO. Nitrate inhibition of burst-forming units-erythroid (BFU-E) colony growth was blocked by PTIO, but not in mature CFU-E. The presented results reveal that NO release and/or production mediates the hydroxyurea inhibition of mature erythroid colony growth and the frequency of iNOS immunoreactive CFU-E.
Improving the Efficacy of Mesenchymal Stem/Stromal-Based Therapy for Treatment of Inflammatory Bowel Diseases
Authors: Lopez-Santalla Mercedes , Garin Marina Inmaculada
Published: October 2021

Inflammatory bowel diseases (IBD) consisting of persistent and relapsing inflammatory processes of the intestinal mucosa are caused by genetic, environmental, and commensal microbiota factors. Despite recent advances in clinical treatments aiming to decrease inflammation, nearly 30% of patients treated with biologicals experienced drawbacks including loss of response, while others can develop severe side effects. Hence, novel effective treatments are highly needed. Mesenchymal stem/stromal cell (MSCs) therapy is an innovative therapeutic alternative currently under investigation for IBD. MSCs have the inherent capacity of modulating inflammatory immune responses as well as regenerating damaged tissues and are therefore a prime candidate to use as cell therapy in patients with IBD. At present, MSC-based therapy has been shown preclinically to modulate intestinal inflammation, whilst the safety of MSC-based therapy has been demonstrated in clinical trials. However, the successful results in preclinical studies have not been replicated in clinical trials. In this review, we will summarize the protocols used in preclinical and clinical trials and the novel approaches currently under investigation which aim to increase the beneficial effects of MSC-based therapy for IBD.
Significance of Sex Differences in ncRNAs Expression and Function in Pregnancy and Related Complications
Authors: Varì Rosaria , Scazzocchio Beatrice , Filardi Tiziana , Citarella Anna , Bellenghi Maria , Masella Roberta , Santangelo Carmela
Published: October 2021

In the era of personalized medicine, fetal sex-specific research is of utmost importance for comprehending the mechanisms governing pregnancy and pregnancy-related complications. In recent times, noncoding RNAs (ncRNAs) have gained increasing attention as critical players in gene regulation and disease pathogenesis, and as candidate biomarkers in human diseases as well. Different types of ncRNAs, including microRNAs (miRNAs), piwi-interacting RNAs (piRNAs), long noncoding RNAs (lncRNAs), and circular RNAs (circRNAs), participate in every step of pregnancy progression, although studies taking into consideration fetal sex as a central variable are still limited. To date, most of the available data have been obtained investigating sex-specific placental miRNA expression. Several studies revealed that miRNAs regulate the (patho)-physiological processes in a sexually dimorphic manner, ensuring normal fetal development, successful pregnancy, and susceptibility to diseases. Moreover, the observation that ncRNA profiles differ according to cells, tissues, and developmental stages of pregnancy, along with the complex interactions among different types of ncRNAs in regulating gene expression, strongly indicates that more studies are needed to understand the role of sex-specific ncRNA in pregnancy and associated disorders.
Role of PARP in TNBC: Mechanism of Inhibition, Clinical Applications, and Resistance
Authors: Singh Desh Deepak , Parveen Amna , Yadav Dharmendra Kumar
Published: October 2021

Triple-negative breast cancer is a combative cancer type with a highly inflated histological grade that leads to poor theragnostic value. Gene, protein, and receptor-specific targets have shown effective clinical outcomes in patients with TNBC. Cells are frequently exposed to DNA-damaging agents. DNA damage is repaired by multiple pathways; accumulations of mutations occur due to damage to one or more pathways and lead to alterations in normal cellular mechanisms, which lead to development of tumors. Advances in target-specific cancer therapies have shown significant momentum; most treatment options cause off-target toxicity and side effects on healthy tissues. PARP (poly(ADP-ribose) polymerase) is a major protein and is involved in DNA repair pathways, base excision repair (BER) mechanisms, homologous recombination (HR), and nonhomologous end-joining (NEJ) deficiency-based repair mechanisms. DNA damage repair deficits cause an increased risk of tumor formation. Inhibitors of PARP favorably kill cancer cells in BRCA-mutations. For a few years, PARPi has shown promising activity as a chemotherapeutic agent in BRCA1- or BRCA2-associated breast cancers, and in combination with chemotherapy in triple-negative breast cancer. This review covers the current results of clinical trials testing and future directions for the field of PARP inhibitor development.
Single-Cell Analysis Using Machine Learning Techniques and Its Application to Medical Research
Authors: Asada Ken , Takasawa Ken , Machino Hidenori , Takahashi Satoshi , Shinkai Norio , Bolatkan Amina , Kobayashi Kazuma , Komatsu Masaaki , Kaneko Syuzo , Okamoto Koji , Hamamoto Ryuji
Published: October 2021

In recent years, the diversity of cancer cells in tumor tissues as a result of intratumor heterogeneity has attracted attention. In particular, the development of single-cell analysis technology has made a significant contribution to the field; technologies that are centered on single-cell RNA sequencing (scRNA-seq) have been reported to analyze cancer constituent cells, identify cell groups responsible for therapeutic resistance, and analyze gene signatures of resistant cell groups. However, although single-cell analysis is a powerful tool, various issues have been reported, including batch effects and transcriptional noise due to gene expression variation and mRNA degradation. To overcome these issues, machine learning techniques are currently being introduced for single-cell analysis, and promising results are being reported. In addition, machine learning has also been used in various ways for single-cell analysis, such as single-cell assay of transposase accessible chromatin sequencing (ATAC-seq), chromatin immunoprecipitation sequencing (ChIP-seq) analysis, and multi-omics analysis; thus, it contributes to a deeper understanding of the characteristics of human diseases, especially cancer, and supports clinical applications. In this review, we present a comprehensive introduction to the implementation of machine learning techniques in medical research for single-cell analysis, and discuss their usefulness and future potential.
A Live Probiotic Vaccine Prototype Based on Conserved Influenza a Virus Antigens Protect Mice against Lethal Influenza Virus Infection
Authors: Mezhenskaya Daria , Isakova-Sivak Irina , Gupalova Tatiana , Bormotova Elena , Kuleshevich Eugenia , Kramskaya Tatiana , Leontieva Galina , Rudenko Larisa , Suvorov Alexander
Published: October 2021

Background: Due to the highly variable nature of the antigenic properties of the influenza virus, many efforts have been made to develop broadly reactive influenza vaccines. Various vaccine platforms have been explored to deliver conserved viral antigens to the target cells to induce cross-reactive immune responses. Here, we assessed the feasibility of using Enterococcus faecium L3 as a bacterial vector for oral immunization against influenza virus. Methods: we generated two vaccine prototypes by inserting full-length HA2 (L3-HA2) protein or its long alpha helix (LAH) domain in combination with four M2e tandem repeats (L3-LAH+M2e) into genome of E.faecium L3 probiotic strain. The immunogenicity and protective potential of these oral vaccines were assessed in a lethal challenge model in BALB/c mice. Results: as expected, both vaccine prototypes induced HA stem-targeting antibodies, whereas only L3-LAH+4M2e vaccine induced M2e-specific antibody. The L3-HA2 vaccine partially protected mice against lethal challenge with two H1N1 heterologous viruses, while 100% of animals in the L3-LAH+4M2e vaccine group survived in both challenge experiments, and there was significant protection against weight loss in this group, compared to the L3 vector-immunized control mice. Conclusions: the recombinant enterococcal strain L3-LAH+4M2e can be considered as a promising live probiotic vaccine candidate for influenza prevention and warrants further evaluation in relevant pre-clinical models.
Reduction in Epigenetic Age Acceleration Is Related to Empathy in Mothers with Neglectful Caregiving
Authors: Herrero-Roldán Silvia , Rodrigo María José , Hernández-Cabrera Juan A. , Mitchell Colter , López Maykel , Alcoba-Florez Julia , Fisher Jonah , Espinosa Fernanda , León Inmaculada
Published: October 2021

DNA methylation clocks are used as molecular estimators of epigenetic age, but with little evidence in mothers and none in neglectful mothering. We investigated differences in epigenetic age acceleration (EAA) and the role of empathy using the PhenoAge clock. We collected saliva samples from mothers with extreme disregard for their child’s needs (50 in the neglect group, NG) and mothers with non-neglectful caregiving (87 in the control group, CG). Mothers completed an empathy scale, along with questionnaires of their own childhood maltreatment, adverse life events and psychiatric disorders. Sociodemographic variables potentially affecting EAA were also measured. The ANCOVA solution showed a significant increase in EAA in the NG compared to the CG, after adjustment for maternal age, number of pregnancies, financial assistance, adverse events, childhood maltreatment and psychiatric disorder. The group interaction effects showed a reduction in EAA for greater empathic concern and for a higher education level both as positive factors, and an increment in EAA for mothers living in a two-parent family as a risk factor, all in the NG. Our findings open the search for protective factors of EAA associated with caregiver behavior to reduce health vulnerabilities and poor social functioning, especially for mothers at risk of maladaptive caregiving.
Down Syndrome: Neuropsychological Phenotype across the Lifespan
Authors: Pulsifer Margaret
Published: October 2021

Down syndrome (DS), caused by triplication of chromosome 21, is the most common genetic cause of intellectual disability (ID), with an estimated incidence of one in 700 live births [...]
Advances in Reproductive Endocrinology and Neuroendocrine Research Using Catfish Models
Authors: Senthilkumaran Balasubramanian , Kar Sonika
Published: October 2021

Catfishes, belonging to the order siluriformes, represent one of the largest groups of freshwater fishes with more than 4000 species and almost 12% of teleostean population. Due to their worldwide distribution and diversity, catfishes are interesting models for ecologists and evolutionary biologists. Incidentally, catfish emerged as an excellent animal model for aquaculture research because of economic importance, availability, disease resistance, adaptability to artificial spawning, handling, culture, high fecundity, hatchability, hypoxia tolerance and their ability to acclimate to laboratory conditions. Reproductive system in catfish is orchestrated by complex network of nervous, endocrine system and environmental factors during gonadal growth as well as recrudescence. Lot of new information on the molecular mechanism of gonadal development have been obtained over several decades which are evident from significant number of scientific publications pertaining to reproductive biology and neuroendocrine research in catfish. This review aims to synthesize key findings and compile highly relevant aspects on how catfish can offer insight into fundamental mechanisms of all the areas of reproduction and its neuroendocrine regulation, from gametogenesis to spawning including seasonal reproductive cycle. In addition, the state-of-knowledge surrounding gonadal development and neuroendocrine control of gonadal sex differentiation in catfish are comprehensively summarized in comparison with other fish models.
Comparison of Three CD3-Specific Separation Methods Leading to Labeled and Label-Free T Cells
Authors: Weiss Ronald , Gerdes Wilhelm , Berthold Rommy , Sack Ulrich , Koehl Ulrike , Hauschildt Sunna , Grahnert Anja
Published: October 2021

T cells are an essential part of the immune system. They determine the specificity of the immune response to foreign substances and, thus, help to protect the body from infections and cancer. Recently, T cells have gained much attention as promising tools in adoptive T cell transfer for cancer treatment. However, it is crucial not only for medical purposes but also for research to obtain T cells in large quantities, of high purity and functionality. To fulfill these criteria, efficient and robust isolation methods are needed. We used three different isolation methods to separate CD3-specific T cells from leukocyte concentrates (buffy coats) and Ficoll purified PBMCs. To catch the target cells, the Traceless Affinity Cell Selection (TACS®) method, based on immune affinity chromatography, uses CD-specific low affinity Fab-fragments; while the classical Magnetic Activated Cell Sorting (MACS®) method relies on magnetic beads coated with specific high affinity monoclonal antibodies. The REAlease® system also works with magnetic beads but, in contrast to MACS®, low-affinity antibody fragments are used. The target cells separated by TACS® and REAlease® are “label-free”, while cells isolated by MACS® still carry the cell specific label. The time required to isolate T cells from buffy coat by TACS® and MACS® amounted to 90 min and 50 min, respectively, while it took 150 min to isolate T cells from PBMCs by TACS® and 110 min by REAlease®. All methods used are well suited to obtain T cells in large quantities of high viability (>92%) and purity (>98%). Only the median CD4:CD8 ratio of approximately 6.8 after REAlease® separation differed greatly from the physiological conditions. MACS® separation was found to induce proliferation and cytokine secretion. However, independent of the isolation methods used, stimulation of T cells by anti CD3/CD28 resulted in similar rates of proliferation and cytokine production, verifying the functional activity of the isolated cells.
Development of Electrochemical Sensors/Biosensors to Detect Natural and Synthetic Compounds Related to Agroalimentary, Environmental and Health Systems in Argentina. A Review of the Last Decade
Authors: Granero Adrian M. , Fernández Héctor , Zon María A. , Robledo Sebastián N. , Pierini Gastón D. , Di Tocco Aylen , Carrillo Palomino Roodney A. , Maccio Sabrina , Riberi Walter I. , Arévalo Fernando J.
Published: October 2021

Electrochemical sensors and biosensors are analytical tools, which are in continuous development with the aim of generating new analytical devices which are more reliable, cheaper, faster, sensitive, selective, and robust than others. In matrices related to agroalimentary, environmental, or health systems, natural or synthetic compounds occur which fulfil specific roles; some of them (such as mycotoxins or herbicides) may possess harmful properties, and others (such as antioxidants) beneficial ones. This imposes a challenge to develop new tools and analytical methodologies for their detection and quantification. This review summarises different aspects related to the development of electrochemical sensors and biosensors carried out in Argentina in the last ten years for application in agroalimentary, environmental, and health fields. The discussion focuses on the construction and development of electroanalytical methodologies for the determination of mycotoxins, herbicides, and natural and synthetic antioxidants. Studies based on the use of different electrode materials modified with micro/nanostructures, functional groups, and biomolecules, complemented by the use of chemometric tools, are explored. Results of the latest reports from research groups in Argentina are presented. The main goals are highlighted.
Genetic Variants of DMBT1 and SFTPD and Disease Severity in Paediatric Inflammatory Bowel Disease—A Polish Population-Based Study
Authors: Glapa-Nowak Aleksandra , Szczepanik Mariusz , Banaszkiewicz Aleksandra , Iwańczak Barbara , Kwiecień Jarosław , Szaflarska-Popławska Anna , Grzybowska-Chlebowczyk Urszula , Osiecki Marcin , Kierkuś Jarosław , Banasiuk Marcin , Banasiewicz Tomasz , Madsen Jens , Walkowiak Jarosław
Published: October 2021

Deleted in malignant brain tumours 1 protein (DMBT1) and surfactant protein D (SFTPD) are antimicrobial peptides previously linked to inflammatory bowel disease (IBD) susceptibility. This study attempts to link the most potential IBD-associated polymorphisms in DMBT1 and SFTPD with the disease severity in children. A total of 406 IBD patients (Crohn’s disease (CD) n = 214 and ulcerative colitis (UC) n = 192) were genotyped using hydrolysis probe assay. Clinical expression was described by disease activity scales, albumin and C-reactive protein levels, localisation and behaviour (Paris classification), systemic steroid, immunosuppressive, biological, and surgical treatment, number of exacerbation-caused hospitalisations, relapses and nutritional status. IBD patients with the risk genotype (AA) in DMBT1 rs2981804 had more frequent biological treatment (AA: vs. AG/GG; p = 0.012), concomitant diseases (AA vs. AG vs. GG; p = 0.015) and cutaneous manifestations (AA vs. AG/GG, p = 0.008). In UC, rs2981804 genotypes might be linked with albumin concentrations at diagnosis (AA vs. AG vs. GG; p = 0.009). In CD, DMBT1 rs2981745 was significantly associated with the number of severe relapses per year of disease (p = 0.020) and time-to-immunosuppression (p = 0.045). SFTPD was seemingly found to be associated with age at first immunosuppression in IBD (CC vs. CT vs. TT; p = 0.048). In conclusion, selected polymorphisms of DMBT1 and SFTPD might be associated with some disease severity measures in children with IBD. However, the magnitude of associations and their clinical relevance might be minor.
Impact of the Cross-Curricular Education Program on Food Intake, Physical Activity, and Body Mass Index of School Children in a Local Community in Northern Serbia
Authors: Šumonja Sanja , Jevtić Marija
Published: October 2021

Introduction: The trends in the state of nutrition and physical activity of school-age children in Serbia impose a need to create and evaluate programs to improve their nutrition and physical activity. The purpose of this study was to evaluate the impact of the cross-curricular nutrition and physical activity education program (NPAEP) on food intake, physical activity, and body mass index in first–fourth-grade primary school children. Material and methods: The study used an experimental pre-test (t1) post-test (t2) design. The sample included 167 participants in t1 and 178 in t2. The differences in food intake, physical activity, and body mass index before and after implementation of the cross-curricular nutrition and physical activity education program were analyzed using the Mann–Whitney U test, the Chi-squared test, and the Wilcoxon signed-rank test. Values p < 0.05 were considered statistically significant. Results: Fruit (t1 = 0.70 ± 0.55; t2 = 1.26 ± 0.65; p < 0.001) and dairy intake (t1 = 1.52; t2 = 1.79; p = 0.005) significantly increased in the intervention group. A significant reduction in time spent watching television (t1 = 78.0; t2 = 56.7; p = 0.005) and playing on the computer (t1 = 32.3; t2 = 25.8; p = 0.047) was achieved in the intervention group. Time spent in organized sports activities significantly increased only in the intervention group (t1 = 21.9; t2 = 30.5; p = 0.046). Body mass index did not change significantly in the intervention group after the implementation of the NPAEP. Conclusions: The nutrition and physical activity education program contributed to an increase in fruit and dairy intake and time spent in intense physical activities and a reduction in screen time. The presented results justify the application of the NPAEP in improving the nutrition and physical activity of first–fourth-grade primary school children.
Outpatient Nephrotoxic Medication Prescription after Pediatric Intensive Care Acute Kidney Injury
Authors: Lefebvre Claire , Dorais Marc , Hessey Erin , Zappitelli Michael
Published: October 2021

Background: Nephrotoxic medication (NTM) avoidance may prevent further kidney damage in children with acute kidney injury (AKI). We compared outpatient NTM prescriptions in children with or without AKI during pediatric intensive care (PICU) hospitalization. We hypothesize that children with AKI are prescribed NTMs at the same rate as those without it. Methods: This was a retrospective administrative data study of children <18 years, admitted to two PICUs in Montreal, Canada, from 2003 to 2005, with ≥30 days of provincial drug coverage. We evaluated the presence of ≥3 outpatient NTM prescriptions during the first year and 5 years after PICU discharge. Results: Of 970 children, 23% had PICU AKI. In the 1st–5th years after discharge, 18% AKI vs. 10% non-AKI and 13% AKI vs. 4% non-AKI patients received ≥3 NTM prescriptions, respectively. There was no association between PICU AKI and prescription of ≥3 NTMs during the first year (adjusted RR 1.02 [95% CI 0.95–1.10]) nor in the first 5 years post-discharge (adjusted RR 1.04 [95%CI 0.96–1.12]). Conclusions: By offering a better understanding of the current state of outpatient NTM prescription to children with AKI, our study is a step toward considering strategies such as knowledge translation interventions for decreasing NTM exposure and improving outcomes in children with AKI.
The Impact of Climate Change on the Reliability of Water Resources
Authors: Sýs Vojtěch , Fošumpaur Pavel , Kašpar Tomáš
Published: October 2021

Climate change impact assessment is crucial for strategic planning in many areas, including water management, agriculture and forestry. Water planning has a long tradition in the Czech Republic, who has implemented the requirements of the Water Framework Directive since 2000. Following the expected impacts of climate change on the hydrological regime, adaptation measures in the water sector are being prepared as part of strategic plans. This contribution studies the uncertainty propagation of climate scenarios in hydrological data, which are then used to assess the reliability of water resources and to design appropriate adaptation measures. The results are being discussed for a case study in the deficit area of Rakovnický stream and Blšanska river basins, which are among the driest areas in the Czech Republic. Research of the impact of climate change on the reliability of water resources has been prepared using ensembles of selected regional climate models. This approach has allowed a probabilistic assessment of the impact on the hydrology regime and the reliability of water supply from reservoirs for various time horizons of climate change. In view of the relatively large variance of potential impacts on water resources, options for further strategic planning in the water management area are being discussed.
Harnessing Chlorophyll Fluorescence for Phenotyping Analysis of Wild and Cultivated Tomato for High Photochemical Efficiency under Water Deficit for Climate Change Resilience
Authors: Sperdouli Ilektra , Mellidou Ifigeneia , Moustakas Michael
Published: October 2021

Fluctuations of the weather conditions, due to global climate change, greatly influence plant growth and development, eventually affecting crop yield and quality, but also plant survival. Since water shortage is one of the key risks for the future of agriculture, exploring the capability of crop species to grow with limited water is therefore fundamental. By using chlorophyll fluorescence analysis, we evaluated the responses of wild tomato accession Solanum pennellii LA0716, Solanum lycopersicum cv. Μ82, the introgression line IL12-4 (from cv. M82 Χ LA0716), and the Greek tomato cultivars cv. Santorini and cv. Zakinthos, to moderate drought stress (MoDS) and severe drought stress (SDS), in order to identify the minimum irrigation level for efficient photosynthetic performance. Agronomic traits (plant height, number of leaves and root/shoot biomass), relative water content (RWC), and lipid peroxidation, were also measured. Under almost 50% deficit irrigation, S. pennellii exhibited an enhanced photosynthetic function by displaying a hormetic response of electron transport rate (ETR), due to an increased fraction of open reaction centers, it is suggested to be activated by the low increase of reactive oxygen species (ROS). A low increase of ROS is regarded to be beneficial by stimulating defense responses and also triggering a more oxidized redox state of quinone A (QA), corresponding in S. pennellii under 50% deficit irrigation, to the lowest stomatal opening, resulting in reduction of water loss. Solanumpennellii was the most tolerant to drought, as it was expected, and could manage to have an adequate photochemical function with almost 30% water regime of well-watered plants. With 50% deficit irrigation, cv. Μ82 and cv. Santorini did not show any difference in photochemical efficiency to control plants and are recommended to be cultivated under deficit irrigation as an effective strategy to enhance agricultural sustainability under a global climate change. We conclude that instead of the previously used Fv/Fm ratio, the redox state of QA, as it can be estimated by the chlorophyll fluorescence parameter 1 - qL, is a better indicator to evaluate photosynthetic efficiency and select drought tolerant cultivars under deficit irrigation.
Review of Residual Stress Impingement Methods to Mitigate Environmental Fracture Susceptibility
Authors: McMahon Matthew E.
Published: October 2021

Environmental cracking- and fatigue-related failures threaten all major industries and, to combat such degradation, numerous residual stress impingement (RSI) methods have been developed with varying levels of efficacy and ease of use. Some of the most commonly used RSI methods, such as shot peening, laser shock peening, and low plasticity burnishing, as well as new methods, such as ultrasonic nanocrystal surface modification, are reviewed in the context of corrosion, corrosion fatigue, and environmental cracking mitigation. The successes and limitations of these treatments are discussed, with a focus on their efficacy against these three damage modes based on the available literature. Case studies are reviewed that demonstrate how these treatments have been adopted and advanced by industry, and application-specific research efforts are explored with a focus on future opportunities. Research is identified that illustrates how the utility of these surface treatments may vary between alloy systems, and where the benefits must be weighed against the risks to a component’s service performance.
Predictive Role of the p16 Immunostaining Pattern in Atypical Cervical Biopsies with Less Common High Risk HPV Genotypes
Authors: Cabibi Daniela , Napolitano Caterina , Giannone Antonino Giulio , Micciulla Maria Carmela , Porcasi Rossana , Lo Coco Roberta , Bosco Liana , Vinciguerra Manlio , Capra Giuseppina
Published: October 2021

P16 immunostaining is considered a useful surrogate of transcriptionally active high-risk (hr) HPV infection. Only strong and widespread “block-like” immunoreactivity is considered specific, whereas weak/focal p16 positive immunostaining is considered not specific, and follow-up and HPV molecular detection is not indicated. The aim of the study was to evaluate the presence of HPV DNA and Ki67 immunostaining in 40 cervical atypical biopsies (CALs) with mild and focal histological features suggestive of HPV infection—20 cases with weak/focal p16 positive immunoreactivity and 20 cases negative for p16 expression. In 16/20 weak/focal p16 positive CALs (80%), the INNO-LiPA HPV genotyping detected hrHPV genotypes (HPV 31, 51, 56, 59, 26, 53, 66, 73, and 82). Co-infection of two or more hrHPV genotypes was often evidenced. HPV16 and 18 genotypes were never detected. Ki67 immunostaining was increased in 10/20 cases (50%). In 19/20 p16 negative CALs, hrHPV infection was absent and Ki67 was not increased. These results suggest that weak/focal p16 immunostaining represents the early stage of transcriptionally active infection, strongly related to the presence of less common hrHPV genotypes, probably with a slower transforming power, but with a potential risk of progression if the infection persists. HPV DNA genotyping and follow-up could be useful in these cases to verify if they are able to evolve into overt dysplastic changes and to improve knowledge of less common hrHPV genotypes.
Quantitative Assessment of Three-Dimensional Choroidal Vascularity and Choriocapillaris Flow Signal Voids in Myopic Patients Using SS-OCTA
Authors: Xu Amin , Sun Gongpeng , Duan Chaoye , Chen Zhen , Chen Changzheng
Published: October 2021

Purpose: To compare the choroidal vascularity of large- and middle-sized choroidal vessels and choriocapillaris (CC) perfusion in patients with different degrees of myopia using swept-source optical coherence tomography angiography (SS-OCTA). Methods: One hundred and thirteen people with myopia were enrolled. SS-OCTA was performed to analyze the choroidal vascularity and CC perfusion. Three-dimensional (3D) choroidal vascularity index (CVI) and choroidal luminal volumes (LV) were obtained by artificial intelligence segmentation of the choroidal lumen in Volume OCT images. CC perfusion was assessed by flow signal voids (FSVs). Results: In the macular, multiple linear regression model showed that choroidal thickness (CT), total choroidal volume, LV, and choroidal stromal volume were negatively correlated with axis length (AL), respectively (all p < 0.001). Three dimensional CVI was negatively associated with AL (p < 0.05). FSV% was positively correlated with age only (p < 0.001). Additionally, after adjustment for age and AL, FSV% had a significant negative correlation with CT (p < 0.05). Conclusion: Choroidal vascularity decreases gradually with increasing severity of myopia. The decrease of CC blood perfusion was related to a higher severity of myopia and the thinning of choroid.
Comparative Performance of Four Established Neonatal Disease Scoring Systems in Predicting In-Hospital Mortality and the Potential Role of Thromboelastometry
Authors: Sokou Rozeta , Tritzali Maroula , Piovani Daniele , Konstantinidi Aikaterini , Tsantes Andreas G. , Ioakeimidis Georgios , Lampridou Maria , Parastatidou Stavroula , Iacovidou Nicoletta , Kokoris Styliani , Nikolopoulos Georgios K. , Kopterides Petros , Bonovas Stefanos , Tsantes Argirios E.
Published: October 2021

Background: To compare the prognostic accuracy of the most commonly used indexes of mortality over time and evaluate the potential of adding thromboelastometry (ROTEM) results to these well-established clinical scores. Methods: The study population consisted of 473 consecutive term and preterm critically-ill neonates. On the first day of critical illness, modified Neonatal Multiple Organ Dysfunction (NEOMOD) scoring system, Score for Neonatal Acute Physiology (SNAP II), Perinatal extension of SNAP (SNAPPE), and SNAPPE II, were calculated and ROTEM standard extrinsically activated (EXTEM) assay was performed simultaneously. Time-to-event methodology for competing-risks was used to assess the performance of the aforementioned indexes in predicting in-hospital mortality over time. Time-dependent receiver operator characteristics curves for censored observation were compared across indexes. The addition of EXTEM parameters to each index was tested in terms of discrimination capacity. Results: The modified NEOMOD score performed similarly to SNAPPE. Both scores performed significantly better than SNAP II and SNAPPE II. Amplitude recorded at 10 min (A10) was the EXTEM parameter most strongly associated with mortality (A10 < 37 mm vs. ≥37 mm; sHR = 5.52; p < 0.001). Adding A10 to each index apparently increased the prognostic accuracy in the case of SNAP II and SNAPPE II. However, these increases did not reach statistical significance. Conclusion: Although the four existing indexes considered showed good to excellent prognostic capacity, modified NEOMOD and SNAPPE scores performed significantly better. Though larger studies are needed, adding A10 to well-established neonatal severity scores not including biomarkers of coagulopathy might improve their prediction of in-hospital mortality.
Clinical Manifestations, Pathogenesis, Diagnosis and Treatment of Peripheral Neuropathies in Connective Tissue Diseases: More Diverse and Frequent in Different Subtypes than Expected
Authors: Jin Lei , Liu Yu
Published: October 2021

Purpose of review: To discuss and summarize recent findings in peripheral neuropathy (PN) related to connective tissue diseases (CTD) including its prevalence, clinical manifestations, pathogenesis, diagnosis and treatment. Recent findings: Although PN is a common complication in CTD and has been well studied, recent research has shown that PN is more diverse and frequent in different subtypes of CTD than was expected. The incidence of PN in Sjögren’s syndrome and rheumatoid arthritis (RA) varies according to different disease subtypes, and the pathogenesis of neuropathic pain in different subtypes of eosinophilic granulomatosis with polyangiitis (EGPA) may also differ. Neurogenic inflammation, autoantibody-mediated changes, ischemia of the vascular wall and metabolic mechanisms have been shown to contribute to the pathogenesis of PN in CTD. Moreover, allergic inflammation has been recently identified as a possible new mechanism producing peripheral neuropathic pain associated with MPO-ANCA negative EGPA patients. Glucocorticoids are routinely used to relieve pain caused by PN. However, these steroids may cause hyperalgesia, exacerbate neuropathic pain, and activate the early phase of pain induction and produce hyperalgesia. Recently, neuroactive steroids, such as progesterone, tetrahydroprogesterone and testosterone, have been shown to exert protective effects for several PN symptoms, and in particular neuropathic pain. Neuroactive steroids will be an interesting topic for future research into PN in CTD. Summary: It is essential for the diagnosis and treatment of PN in CTD to be updated. Timely diagnosis, appropriate treatments, and multidisciplinary care are essential to minimize morbidity and decrease the risk of permanent neurologic deficits. Further studies are needed to guide diagnosis and treatment.
Contact Angle and Cell Adhesion of Micro/Nano-Structured Poly(lactic-co-glycolic acid) Membranes for Dental Regenerative Therapy
Authors: Kaga Naoyuki , Fujimoto Hiroki , Morita Sho , Yamaguchi Yuichiro , Matsuura Takashi
Published: October 2021

Biodegradable membranes are used in regenerative dentistry for guided tissue regeneration (GTR) and guided bone regeneration (GBR). In this study, patterned poly(lactic-co-glycolic acid) (PLGA) membranes with groove, pillar, and hole structures were successfully fabricated by thermal nanoimprinting. Their surfaces were evaluated for topography by scanning electron microscopy and laser microscopy, for hydrophobicity/hydrophilicity by contact angle analysis, and for MC3T3-E1 cell adhesion. The sizes of the patterns on the surfaces of the membranes were 0.5, 1.0, and 2.0 μm, respectively, with the height/depth being 1.0 μm. The pillared and holed PLGA membranes were significantly more hydrophobic than the non-patterned PLGA membranes (p < 0.05). However, the 0.5 μm- and 1.0 μm-grooved PLGA membranes were significantly more hydrophilic than the non-patterned PLGA membranes (p < 0.05). The 0.5 μm-grooved, pillared, and holed membranes exhibited significantly superior adhesion to the MC3T3-E1 cells than the non-patterned PLGA (p < 0.05). These results suggest that patterned PLGA membranes can be clinically used for GTR and GBR in the dental regeneration field.
Secure OFDM with Peak-to-Average Power Ratio Reduction Using the Spectral Phase of Chaotic Signals
Authors: Haroun Mohamad F. , Gulliver T. Aaron
Published: October 2021

In this paper, a new physical layer security technique is proposed for Orthogonal Frequency Division Multiplexing (OFDM) communication systems. The security is achieved by modifying the OFDM symbols using the phase information of chaos in the frequency spectrum. In addition, this scheme reduces the Peak to Average Power Ratio (PAPR), which is one of the major drawbacks of OFDM. The Selected Mapping (SLM) technique for PAPR reduction is employed to exploit the random characteristics of chaotic sequences. The reduction with this algorithm is shown to be similar to that of other SLM schemes, but it has lower computational complexity and side information does not have to be sent to the receiver. The security of this technique stems from the noise like behavior of chaotic sequences and their dependence on the initial conditions of the chaotic generator (which are used as the key). Even a slight difference in the initial conditions will result in a different phase sequence, which prevents an eavesdropper from recovering the transmitted OFDM symbols.
Multidimensional Aspect of Corporate Entrepreneurship in Family Business and SMEs: A Systematic Literature Review
Authors: Wahyudi Indra , Suroso Arif Imam , Arifin Bustanul , Syarief Rizal , Rusli Meika Syahbana
Published: October 2021

This study aimed to review and analyze corporate entrepreneurship (CE) within family businesses and small and medium enterprises (SMEs), providing a review of the current state of research and suggesting a future research agenda. In a systematic literature review, 1040 articles indexed in Scopus were initially subjected to bibliometric and qualitative analysis. Finally, 53 papers published in various academic journals, focusing on corporate entrepreneurship, family businesses, and SMEs, were subjected to bibliometric analysis and qualitative research to identify the new potential state of the art in corporate entrepreneurship. CE in family businesses and SMEs is not a general research trend in the entrepreneurial area. This research shows that the literature on CE models and tools in family businesses and SMEs is still sparse. The review results correlate the actors of CE, behavior, and activities, including entrepreneurial orientation, entrepreneurial management, and entrepreneurial leadership and performance, as outcomes in family business and SMEs.
Holiday Rentals in Cultural Tourism Destinations: A Comparison of Booking.com-Based Daily Rate Estimation for Seville and Porto
Authors: Solano-Sánchez Miguel Ángel , Santos José António C. , Custódio Santos Margarida , Fernández-Gámez Manuel Ángel
Published: October 2021

Multiple variables determine holiday rentals’ price composition in cultural tourism destinations. This study sought, first, to test a model including the variables with the greatest impact on tourism accommodations’ prices in these destinations and, second, to demonstrate the proposed model’s applicability to cultural city destinations by identifying the adaptations needed to apply it to different contexts. Two cities were selected for the model application—Seville in Spain and Porto in Portugal—both of which are located in different countries and are well-known cultural tourism destinations. The data were extracted from Booking.com because this accommodations platform has adapted its offer to the sharing economy, becoming one of the most important players in the market, and because research on holiday rentals using data from Booking.com is scarce. The results show that the variables used are relevant and highlight the adaptations necessary for specific cultural tourism destinations, thereby indicating that the model can be applied to all cultural tourism destinations. The proposed approach can help holiday rental managers select the correct tools for determining their accommodation units’ daily rates according to their product and marketing context’s characteristics.
The IARA Model Proved Effective in Emotional Literacy, Characters Strengths Awareness, and Cohesion among Italian Children
Authors: Maulini Claudia , Kuvačić Goran , Savani Wlady , Zanelli Vanessa , Padovan Anna , Bocciolesi Enrico , De Giorgio Andrea
Published: October 2021

Literature highlights how education in the twenty-first century begins to advocate multiple new concepts, such as new technology, new pedagogies, interdisciplinary curricula, open learning, etc. Among these concepts, the recognition and awareness about one’s character strengths are demonstrated to improve emotional management and individual/group’s quality of life. We studied three fifth-grade Italian classes using an emerging educational model called IARA to verify if it can truly improve emotional literacy, characters strengths awareness, and cohesion in ten-years children. To demonstrate its efficacy, we used Bloom’s Taxonomy and Sociogrammatic study. We proved that the IARA could be an efficient model to improve one’s character strengths and class milieu awareness.
Social Media Tools for Educational Sustainability in Conflict-Affected Regions
Authors: Baytiyeh Hoda
Published: October 2021

This conceptual paper demonstrates the potential role of social media in providing students with access to education during emergency situations when schools cannot ensure students’ safety or provide safe learning environments. It is based on conceptual analysis that transforms face-to-face education into a cost-free, online educational environment by relying on social-media learning tools during short-term disruptions caused by violence and conflict. This article proposes a framework that outlines how technology can be used to maintain education in schools during conflicts and emergency situations: cloud computing to access administrative resources and social media tools to maintain teaching/learning resources and student–teacher as well as student–student interactions. The proposed strategy could be greatly beneficial to educational leaders and administrators in regions vulnerable to sectarian conflicts where student safety and the delivery of educational services can become major challenges. This paper contributes to the literature by emphasising the advantages of social media tools for educational delivery in conflict-afflicted regions.
A Bra Monitoring System Using a Miniaturized Wearable Ultra-Wideband MIMO Antenna for Breast Cancer Imaging
Authors: Mahmood Sarmad Nozad , Ishak Asnor Juraiza , Jalal Ali , Saeidi Tale , Shafie Suhaidi , Soh Azura Che , Imran Muhammad Ali , Abbasi Qammer H.
Published: October 2021

This paper represents a miniaturized, dual-polarized, multiple input–multiple output (MIMO) wearable antenna. A vertically polarized, leaf-shaped antenna and a horizontally polarized, tree-shaped antenna are designed, and the performance of each antenna is investigated. After designing the MIMO antenna, it is loaded with stubs, parasitic spiral, and shorting pins to reduce the coupling effects and remove the unwanted resonances. Afterward, the two-port MIMO cells are spaced by 2 mm and rotated by 90° to create three more cells. The antennas are designed using two layers of denim and felt substrates with dielectric constants of 1.2 and 1.8, and thicknesses of 0.5 mm and 0.9 mm, respectively, along with the ShieldIt™ conductive textile. The antenna covers a bandwidth of 4.8–30 GHz when the specific absorption rate (SAR) meets the 1 g and 10 g standards. Isolation greater than 18 dB was obtained and mutual coupling was reduced after integrating shorting pins and spiral parasitic loadings. A maximum radiation efficiency and directive gain of 96% and 5.72 dBi were obtained, respectively, with the relatively small size of 11 × 11 × 1.4 mm3 for the single element and final dimensions of 24 × 24 × 1.4 mm3 for the full assembly. The antenna’s performance was examined for both on-body (breast) and free space conditions using near-field microwave imaging. The achieved results such as high fidelity, low SAR, and accuracy in localization of the tumour indicate that the MIMO antenna is a decent candidate for breast cancer imaging.