bluuebunny/crossref_metadata_2025 huggingface dataset:
State-of-the-Art Review of Fluid Catalytic Cracking (FCC) Catalyst Regeneration Intensification Technologies
Authors: Oloruntoba Adefarati , Zhang Yongmin , Hsu Chang Samuel
Published: March 2022

Fluid catalytic cracking (FCC) is the workhorse of modern crude oil refinery. Its regenerator plays a critical role in optimizing the overall profitability by efficiently restoring the catalyst activity and enhancing the heat balance in the riser reactor. Improvement in the device metallurgy and process operations have enabled industrial regenerators to operate at high temperatures with a better coke burning rate and longer operating cycle. Today, the carbon content of regenerated catalyst has drastically reduced to less than 0.1 wt.%. However, the unit is still plagued with operational complexities and insufficient understanding of the underlying dynamic, multiscale intricacies. Recent process-intensification strategies provide insights into regenerator performance improvement potentials. In this review, the importance of the uniform distribution of spent catalysts through structural modification and operational manipulations of the catalyst distributor is discussed. The knowledge of the role of baffles in enhancing excellent gas–solid interaction has been increasing, but skepticism due to its complex hydrodynamic effects on gas–solid flows fends off operators from its application, a critical evaluation of its implication in the regenerators is covered. The understanding of the contribution of air/steam distributor design and feed gas injection techniques for even contact with spent catalyst leading to the improvement in FCC performance is also investigated. The reliability of FCC components is equally a big concern, as unplanned shutdown and enormous economic losses are being witnessed due to device failure. To this end, mitigation approaches to damaging afterburn and high-temperature erosion problems with respect to process control and geometric adjustment in the bed, freeboard, cyclone separators and collection ducts are explored. Emission limits for fluid catalytic cracking unit (FCCU) and products are consistently ratcheting downward; the commingled turnkey solutions to reducing pollutants generation are also reviewed.
Optimal Design of a Hybrid Energy System for Economic and Environmental Sustainability of Onshore Oil and Gas Fields
Authors: Bishnoi Deepika , Chaturvedi Harsh
Published: March 2022

The pollution caused by gas flaring is hazardous to the health of flora, fauna, and humans settled around the flaring site. Gas flaring also incurs economic loss as natural gas, an energy source, is wasted in flares. Furthermore, the unreliable electrical infrastructure is a roadblock for oil and gas companies attempting to achieve their production targets. This paper presents a framework to design hybrid energy systems (HES) which utilize the gas flare waste along with the locally available renewable energy sources to generate electricity. A novel dispatch strategy to suit the requirements of the oil and gas fields has been used for real-time simulations and optimization of the HES. As a test case, six different hybrid energy configurations, modelled for two gas flaring sites, Lakwa and Geleky in Assam—India, were analyzed and compared on the basis of economic and environmental factors. The best suitable configuration comprised 2000 kW solar photovoltaic (PV) panel sets, one 200 kW gas microturbine, two 30 kW gas microturbines, and grid connection. The proposed system economically outperformed the existing power system in the area by 35.52% in terms of the net present cost. Moreover, it could save 850 tons of carbon dioxide emissions annually, and it has a renewable fraction of 93.7% in the total energy generation. Owing to these merits, the presented technique would be a promising option for generation of electricity from flare gas waste and to mitigate pollution.
Model Supported Business Case Scenario Analysis for Decentral Hydrogen Conversion, Storage and Consumption within Energy Hubs
Authors: van Leeuwen Richard P. , Boerman Annelies E. , Schaefer Edmund W. , Hoogsteen Gerwin , Hajimolana Yashar S.
Published: March 2022

Recently, smart energy hubs with hydrogen conversion and storage have received increased attention in the Netherlands. The hydrogen is to be used for vehicle filling stations, industrial processes and heating. The scientific problem addressed in this paper is the proper sizing of capacities for renewable energy generation, hydrogen conversion and storage in relation to a feasible business case for the energy hub while achieving security of supply. Scenario analysis is often used during the early stages of the energy planning process, and for this an easy-to-use analysis model is required. This paper investigates available modelling approaches and develops an algorithmic modelling method which is worked out in Microsoft Excel and offers ease of use for scenario analysis purposes. The model is applied to case study, which leads to important insights such as the expected price of hydrogen and the proper sizing of electrolyser and hydrogen storage for that case. The model is made available open-source. Future work is proposed in the direction of application of the model for other project cases and comparison of results with other available modelling tools.
Noise Reduction Study of Pressure Pulsation in Pumped Storage Units Based on Sparrow Optimization VMD Combined with SVD
Authors: Ren Yan , Zhang Linlin , Chen Jiangtao , Liu Jinwei , Liu Pan , Qiao Ruoyu , Yao Xianhe , Hou Shangchen , Li Xiaokai , Cao Chunyong , Chen Hongping
Published: March 2022

The unbalanced forces generated by pumped storage units operating under non-ideal operating conditions can cause pressure pulsations. Due to the noise interference, the feature information reflecting the operating state of the unit in the pressure pulsation is difficult to extract. Therefore, this paper proposes a noise reduction method based on sparrow search algorithm (SSA) optimized variational mode decomposition (VMD) combined with singular value decomposition (SVD). Firstly, SSA is used to realize the adaptive optimization of VMD parameters for ideal decomposition of the signal. Then, the noise reduction of the decomposed signal is performed by using the sensitivity of the Permutation Entropy (PE) for small mutations. The noise reduction and reconstruction of the decomposed signal are carried out again by using SVD. The experimental and comparison results show that the mean square error of the signal after VMD-SVD feature extraction is reduced from 1.0068 to 0.0732 and the correlation coefficient is increased from 0.2428 to 0.9614. It is proved that the method achieves better results in the pressure pulsation signal of pumped storage units and has some application significance for the fault diagnosis of pumped storage units.
Energy-Efficient Robot Configuration and Motion Planning Using Genetic Algorithm and Particle Swarm Optimization
Authors: Nonoyama Kazuki , Liu Ziang , Fujiwara Tomofumi , Alam Md Moktadir , Nishi Tatsushi
Published: March 2022

The implementation of Industry 5.0 necessitates a decrease in the energy consumption of industrial robots. This research investigates energy optimization for optimal motion planning for a dual-arm industrial robot. The objective function for the energy minimization problem is stated based on the execution time and total energy consumption of the robot arm configurations in its workspace for pick-and-place operation. Firstly, the PID controller is being used to achieve the optimal parameters. The parameters of PID are then fine-tuned using metaheuristic algorithms such as Genetic Algorithms and Particle Swarm Optimization methods to create a more precise robot motion trajectory, resulting in an energy-efficient robot configuration. The results for different robot configurations were compared with both motion planning algorithms, which shows better compatibility in terms of both execution time and energy efficiency. The feasibility of the algorithms is demonstrated by conducting experiments on a dual-arm robot, named as duAro. In terms of energy efficiency, the results show that dual-arm motions can save more energy than single-arm motions for an industrial robot. Furthermore, combining the robot configuration problem with metaheuristic approaches saves energy consumption and robot execution time when compared to motion planning with PID controllers alone.
Multisystemic Manifestations in Rare Diseases: The Experience of Dyskeratosis Congenita
Authors: Callea Michele , Martinelli Diego , Cammarata-Scalisi Francisco , Grimaldi Chiara , Jilani Houweyda , Grimaldi Piercesare , Willoughby Colin Eric , Morabito Antonino
Published: March 2022

Dyskeratosis congenital (DC) is the first genetic syndrome described among telomeropathies. Its classical phenotype is characterized by the mucocutaneous triad of reticulated pigmentation of skin lace, nail dystrophy and oral leukoplakia. The clinical presentation, however, is heterogeneous and serious clinical complications include bone marrow failure, hematological and solid tumors. It may also involve immunodeficiencies, dental, pulmonary and liver disorders, and other minor complication. Dyskeratosis congenita shows marked genetic heterogeneity, as at least 14 genes are responsible for the shortening of telomeres characteristic of this disease. This review discusses clinical characteristics, molecular genetics, disease evolution, available therapeutic options and differential diagnosis of dyskeratosis congenita to provide an interdisciplinary and personalized medical assessment that includes family genetic counseling.
Fostering Geoethics in Flood Risk Reduction: Lessons Learned from the EU Project LIFE PRIMES
Authors: Casareale Cristina , Gioia Eleonora , Colocci Alessandra , Marchetti Noemi , Carone Maria Teresa , Marincioni Fausto
Published: March 2022

Despite the concrete evidence of human responsibilities with the ongoing environmental crisis, tangible changes toward low disaster-risk development models are slow in coming and delayed in implementation. This paper discusses the principles of geoethics underpinning flood risk reduction by analyzing the results of the EU project LIFE PRIMES (Preventing flooding RIsks by Making resilient communitiES). Through the administration of a questionnaire, issues of flood literacy, effective communication and individual responsibility concerning flood hazard and exposure were investigated. Directly engaging local communities, the LIFE PRIMES project appears to have increased citizens attention toward environmental ethics, thus providing an encouraging perspective for appropriate human–environment interaction.
A Comprehensive Evaluation Method for Soil Remediation Technology Selection: Case Study of Ex Situ Thermal Desorption
Authors: Li Shuang , He Liao , Zhang Bo , Yan Yan , Jiao Wentao , Ding Ning
Published: March 2022

Quantitative evaluation of different contaminated soil remediation technologies in multiple dimensions is beneficial for the optimization and comparative selection of technology. Ex situ thermal desorption is widely used in remediation of organic contaminated soil due to its excellent removal effect and short engineering period. In this study, a comprehensive evaluation method of soil remediation technology, covering 20 indicators in five dimensions, was developed. It includes the steps of constructing an indicator system, accounting for the indicator, normalization, determining weights by analytic hierarchy process, and comprehensive evaluation. Three ex situ thermal desorption technology—direct thermal desorption, indirect thermal desorption, and indirect thermal heap—in China were selected for the model validation. The results showed that the direct thermal desorption had the highest economic and social indicator scores of 0.068 and 0.028, respectively. The indirect thermal desorption had the highest technical and environmental indicator scores of 0.118 and 0.427, respectively. The indirect thermal heap had the highest resource indicator score of 0.175. With balanced performance in five dimensions, the indirect thermal desorption had the highest comprehensive score of 0.707, which is 1.6 and 1.4 times higher than the direct thermal desorption and indirect thermal heap, respectively. The comprehensive evaluation method analyzed and compared the characteristics of the ex situ thermal desorption technology from different perspectives, such as specific indicators, multiple dimensions, and single comprehensive values. It provided a novel evaluation approach for the sustainable development and application of soil remediation technology.
Patient Safety Culture and Its Associated Factors: A Situational Analysis among Nurses in Katsina Public Hospitals, Northwest Nigeria
Authors: Kaware Musa Sani , Ibrahim Mohd Ismail , Shafei Mohd Nazri , Mohd Hairon Suhaily , Abdullahi Abduljaleel Umar
Published: March 2022

Background: Patient safety involves identifying, assessing, and managing patient-related risks and occurrences to improve patient care and reduce patient harm. In Nigeria, there is a lack of studies on patient safety culture, especially in the northern part of the country. This study aimed to determine the levels and factors that contribute to nurses’ negative perceptions of patient safety culture in public health facilities. Methodology: A total of 460 nurses were surveyed across 21 secondary health facilities using the Hospital Survey on Patient Safety Culture, and the response rate was 93.5%. Descriptive statistics and multiple logistic regression were used to analyze the data. Results: The results showed that 59.8% of the respondents were female, and 42.6% were within the age range of 30–39 years old. Most of them (48.3%) had spent 1–5 years working in the hospital. Three out of 12 composite measures had higher negative responses (staffing—30.5%, non-punitive response to error—42.8%, and frequency of events reported—43.1%). A multiple logistic regression analysis affirmed that all three variables, in addition to organizational learning, were significant associated with overall negative perceptions of patient safety culture, with 3.15, 1.84, 2.26, and 2.39 odds ratios, respectively. Conclusion: The results revealed that four critical areas of patient safety required improvement; therefore, intervention is recommended to minimize unnecessary patient harm and medical expenses.
Maternal Factors Affecting the Macronutrient Composition of Transitional Human Milk
Authors: Ryoo Chung Ja , Kang Nam Mi
Published: March 2022

This study investigated the influence of selected maternal factors on the macronutrient composition and energy of human milk (HM). The study enrolled 159 breastfeeding mothers from five postpartum care centers in Seoul, Korea. Their gestational weeks were 37–42 weeks, they had no complications before and after childbirth, and were at 7–14 days postpartum. They provided data using structured questionnaires on general characteristics, stress, sleep quality, spousal support, and dietary intake. Breastfeeding assessment (LATCH) was investigated by qualified nurses, and each mother provided one sample of HM. The HM composition was analyzed using the Miris® HM analyzer. The relationships between variables were analyzed using Pearson’s correlation analysis, and a linear regression analysis was performed to verify the main variables. It was found that maternal dietary intake was related to HM composition as the %energy from carbohydrates (β = 0.86, p < 0.01) and %energy from fat (β = 0.77, p < 0.05) showed positive relationships with HM energy. The LATCH score was positively related to HM energy (β = 0.17, p < 0.05). In contrast, postpartum stress, sleep quality, and spousal support were not associated with HM macronutrient composition. In conclusion, HM macronutrients and energy content were associated with maternal dietary intake and LATCH scores, but not with postpartum stress, sleep quality, and spousal support.
Trends of Multimorbidity Patterns over 16 Years in Older Taiwanese People and Their Relationship to Mortality
Authors: Ho Hsin-En , Yeh Chih-Jung , Wei James Cheng-Chung , Chu Wei-Min , Lee Meng-Chih
Published: March 2022

Understanding multimorbidity patterns is important in finding a common etiology and developing prevention strategies. Our aim was to identify the multimorbidity patterns of Taiwanese people aged over 50 years and to explore their relationship with health outcomes. This longitudinal cohort study used data from the Taiwan Longitudinal Study on Aging. The data were obtained from wave 3, and the multimorbidity patterns in 1996, 1999, 2003, 2007, and 2011 were analyzed separately by latent class analysis (LCA). The association between each disease group and mortality was examined using logistic regression. Four disease patterns were identified in 1996, namely, the cardiometabolic (18.57%), arthritis–cataract (15.61%), relatively healthy (58.92%), and multimorbidity (6.9%) groups. These disease groups remained similar in the following years. After adjusting all the confounders, the cardiometabolic group showed the highest risk for mortality (odds ratio: 1.237, 95% confidence interval: 1.040–1.472). This longitudinal study reveals the trend of multimorbidity among older adults in Taiwan for 16 years. Older adults with a cardiometabolic multimorbidity pattern had a dismal outcome. Thus, healthcare professionals should put more emphasis on the prevention and identification of cardiometabolic multimorbidity.
A Qualitative Description of Resident Physicians’ Understanding of Child Maltreatment: Impacts, Recognition, and Response
Authors: Laupacis Megan , Acai Anita , MacMillan Harriet L. , Vanstone Meredith , Stewart Donna , Dimitropoulos Gina , Kimber Melissa
Published: March 2022

Child maltreatment (CM) is a public health problem with devastating effects on individuals, families, and communities. Resident physicians have varied formal education in CM, and report feeling inadequately trained in identifying and responding to CM. The purpose of this study is to explore residents’ understanding of the impacts of CM, and their perceptions of their role in recognizing and responding to CM to better understand their educational needs. This study analyzed qualitative data obtained from a larger project on family violence education. Twenty-nine resident physicians enrolled in pediatric, family medicine, emergency medicine, obstetrics and gynecology, and psychiatry training programs in Alberta, Ontario, and Québec participated in semi-structured interviews to elicit their ideas, experiences, and educational needs relating to CM. Conventional (inductive) content analysis guided the development of codes and categories. Residents had thorough knowledge about the impacts of CM and their duty to recognize CM, but there was less consistency in how residents understood their role in responding to CM. Residents identified the need for more education about recognizing and responding to CM, and the need for educational content to be responsive to training, patient and family factors, and systemic issues. Despite knowledge about the impacts of CM and laws pertaining to mandated reporting, residents reported challenges with responding to concerns of CM. Findings of this study emphasize the need for better training in response to CM. Future educational interventions should consider a multidisciplinary, experiential approach.
Brain Functional Network and Amino Acid Metabolism Association in Females with Subclinical Depression
Authors: Zhao Shanguang , Khoo Selina , Ng Siew-Cheok , Chi Aiping
Published: March 2022

This study aimed to investigate the association between complex brain functional networks and the metabolites in urine in subclinical depression. Electroencephalography (EEG) signals were recorded from 78 female college students, including 40 with subclinical depression (ScD) and 38 healthy controls (HC). The phase delay index was utilized to construct functional connectivity networks and quantify the topological properties of brain networks using graph theory. Meanwhile, the urine of all participants was collected for non-targeted LC-MS metabolic analysis to screen differential metabolites. The global efficiency was significantly increased in the α-2, β-1, and β-2 bands, while the characteristic path length of β-1 and β-2 and the clustering coefficient of β-2 were decreased in the ScD group. The severity of depression was negatively correlated with the level of cortisone (p = 0.016, r = −0.40). The metabolic pathways, including phenylalanine metabolism, phenylalanine tyrosine tryptophan biosynthesis, and nitrogen metabolism, were disturbed in the ScD group. The three metabolic pathways were negatively correlated (p = 0.014, r = −0.493) with the global efficiency of the brain network of the β-2 band, whereas they were positively correlated (p = 0.014, r = 0.493) with the characteristic path length of the β-2 band. They were mainly associated with low levels of L-phenylalanine, and the highest correlation sparsity was 0.11. The disturbance of phenylalanine metabolism and the phenylalanine, tryptophan, tyrosine biosynthesis pathways cause depressive symptoms and changes in functional brain networks. The decrease in the L-phenylalanine level may be related to the randomization trend of the β-1 frequency brain functional network.
A Pilot Study on RTE Food Purchasing and Food-Related Behaviors of College Students in an Urbanized Area
Authors: Choi Jinkyung
Published: March 2022

Ready-to-eat (RTE) food is widely used, and younger age groups are increasingly purchasing these items. This study investigated college students’ consumption of RTE foods and food-related behaviors such as dietary habits, lifestyle, eating out behaviors, and demographic characteristics. We used quantitative methods to gather data. A questionnaire was developed from previous studies and was self-administered to college students in Daejeon, Republic of Korea. Descriptive analysis, ANOVA, and Chi-square tests were conducted to investigate RTE food consumption behaviors and food-related lifestyle factors. A total of 285 data points was entered for analysis. This study found that BMI and gender significantly impacted dietary habits. Residence type and gender showed significant effects on dining out partners. Furthermore, results showed the time of snack consumption and RTE food were similar, suggesting that a snack was replacing college students’ meals or that they might consider RTE food as a snack, or vice versa. A nutrition intervention program for college students should be implemented to encourage a healthy diet.
Online E-Cigarette Information Exposure and Its Association with E-Cigarette Use among Adolescents in Shanghai, China
Authors: Dai Luojia , He Yaping , Tan Yinliang , Yu Zhiping , Zhu Jingfen
Published: March 2022

E-cigarettes are widely advertised, while the potential risks of e-cigarette use have been reported among adolescents. This study assessed online e-cigarette information exposure and its association with adolescents’ e-cigarette use in Shanghai, China. A total of 12,470 students aged 13–18 years participated. A questionnaire collected information on students’ sociodemographic factors, e-cigarette information exposure, cigarette use, e-cigarette use, and e-cigarette use intention. A multivariate logistic regression was performed to assess correlates of exposure to e-cigarette information and the association between e-cigarette information exposure and e-cigarette use. Overall, 73.9% of students knew about e-cigarettes and the primary sources of information were the internet (42.4%), movies/TV (36.4%), bulletin boards in retail stores or supermarkets (34.9%), advertising flyers (33.9%), and friends (13.8%). Students who had friends using e-cigarettes were curious about e-cigarettes and showed a greater monthly allowance; smokers and females were at a higher risk of social media and website exposure. Moreover, online information exposure (social media exposure, website exposure, and total internet exposure) was significantly associated with the intention to use e-cigarettes. The enforcement of regulations on online e-cigarette content should be implemented. Moreover, efforts to prevent young people from using e-cigarettes may benefit from targeting students at a higher risk of online e-cigarette information exposure.
Do Drinking Norms, Motives, and Drinking Behaviors Differ by Age Group among Korean Women?
Authors: Sohn Aeree , Jang Sarang
Published: March 2022

Background: Drinking norms and motives accumulate with drinking experience; thus, it is likely that related drinking behaviors will differ with age. This study aimed to predict drinking behaviors by age based on drinking norms and motives in a sample of Korean women. Methods: This exploratory study used a nationwide demographically stratified sample including 1057 women aged 19–59 years. Self-report questionnaires assessed participants’ general drinking frequency and quantity, two drinking norms, and five dimensional motives. The data were analyzed using Spss 26. Results: Descriptive and injunctive norms were the predictors that accounted for the greatest variance in drinking frequency, quantity, binge drinking, and high-risk drinking across all age groups (p < 0.001). Descriptive norms predicted all drinking behavior better than injunctive norms and all five motives for all age groups. The effects of each of the five motives differed with age. The enhancement motive was the strongest predictor of the motives for drinking frequency, binge drinking, and high-risk drinking across all age groups. Social and conformity motives predicted only binge drinking. Conclusions: These results suggest that descriptive norms, injunctive norms, and enhancement motives predict drinking behaviors across all age groups, although the relative predictive strength of those variables differed by age.
Anti-Eryptotic Activity of Food-Derived Phytochemicals and Natural Compounds
Authors: Restivo Ignazio , Attanzio Alessandro , Tesoriere Luisa , Allegra Mario , Garcia-Llatas Guadalupe , Cilla Antonio
Published: March 2022

Human red blood cells (RBCs), senescent or damaged due to particular stress, can be removed by programmed suicidal death, a process called eryptosis. There are various molecular mechanisms underlying eryptosis. The most frequent is the increase in the cytoplasmic concentration of Ca2+ ions, later exposure of erythrocytes to oxidative stress, hyperosmotic shock, ceramide formation, stimulation of caspases, and energy depletion. Phosphatidylserine (PS) exposed by eryptotic RBCs due to interaction with endothelial CXC-Motiv-Chemokin-16/Scavenger-receptor, causes the RBCs to adhere to vascular wall with consequent damage to the microcirculation. Eryptosis can be triggered by various xenobiotics and endogenous molecules, such as high cholesterol levels. The possible diseases associated with eryptosis are various, including anemia, chronic kidney disease, liver failure, diabetes, hypertension, heart failure, thrombosis, obesity, metabolic syndrome, arthritis, and lupus. This review addresses and collates the existing ex vivo and animal studies on the inhibition of eryptosis by food-derived phytochemicals and natural compounds including phenolic compounds (PC), alkaloids, and other substances that could be a therapeutic and/or co-adjuvant option in eryptotic-driven disorders, especially if they are introduced through the diet.
Host Restriction Factors Modulating HIV Latency and Replication in Macrophages
Authors: Pagani Isabel , Demela Pietro , Ghezzi Silvia , Vicenzi Elisa , Pizzato Massimo , Poli Guido
Published: March 2022

In addition to CD4+ T lymphocytes, myeloid cells and, particularly, differentiated macrophages are targets of human immunodeficiency virus type-1 (HIV-1) infection via the interaction of gp120Env with CD4 and CCR5 or CXCR4. Both T cells and macrophages support virus replication, although with substantial differences. In contrast to activated CD4+ T lymphocytes, HIV-1 replication in macrophages occurs in nondividing cells and it is characterized by the virtual absence of cytopathicity both in vitro and in vivo. These general features should be considered in evaluating the role of cell-associated restriction factors aiming at preventing or curtailing virus replication in macrophages and T cells, particularly in the context of designing strategies to tackle the viral reservoir in infected individuals receiving combination antiretroviral therapy. In this regard, we will here also discuss a model of reversible HIV-1 latency in primary human macrophages and the role of host factors determining the restriction or reactivation of virus replication in these cells.
Dedifferentiation of Human Cardiac Myofibroblasts Is Independent of Activation of COX-2/PGE2 Pathway
Authors: Luu Vy Tran , Phan Sang , Jin Zhu-Qiu
Published: March 2022

The differentiation of cardiac fibroblasts to myofibroblasts is considered to be a critical step in activation and progression of cardiac fibrosis in heart disease. TGF-β is one of the key cytokines that promotes transition of fibroblasts to myofibroblasts. Dedifferentiation of formed myofibroblasts or reversal of formed myofibroblasts to fibroblasts remains incompletely understood. Prostaglandin E2 (PGE2) has been shown to dedifferentiate human lung myofibroblasts. The role of activation of the COX-2/PGE2 pathway in dedifferentiation of cardiac myofibroblasts remains unknown. Here, we show that phorbol 12-myristate 13-acetate (PMA) but not PGE2 induces dedifferentiation of de novo adult human cardiac myofibroblasts stimulated by TGF-β1 from human cardiac fibroblasts as evidenced by reduced expression of α-smooth muscle actin (α-SMA). PMA remarkably increased endogenous levels of PGE2 in human cardiac myofibroblasts. Pretreatment of myofibroblasts with NS-398, a selective COX-2 inhibitor, and PF-04418948, a selective PGE2 receptor type 2 (EP2) antagonist, had no effect on expression of α-SMA nor abolished the dedifferentiation induced by PMA. Our results indicated that endogenous and exogenous PGE2 has no effects on dedifferentiation of cardiac myofibroblasts. PMA-induced dedifferentiation of cardiac myofibroblast is independent of activation of COX-2 and PGE2 pathway. The mechanism in PMA-induced reversal of cardiac myofibroblasts needs to be explored further.
Effect of Bacterial Infection on Ghrelin Receptor Regulation in Periodontal Cells and Tissues
Authors: Nogueira Andressa V. B. , Nokhbehsaim Marjan , Damanaki Anna , Eick Sigrun , Beisel-Memmert Svenja , Kirschneck Christian , Schröder Agnes , Cirelli Thamiris , Leguizamón Natalia D. P. , Cirelli Joni A. , Deschner James
Published: March 2022

The effect of bacterial infection on the expression of growth hormone secretagogue receptor (GHS-R) was investigated in periodontal cells and tissues, and the actions of ghrelin were evaluated. GHS-R was assessed in periodontal tissues of rats with and without periodontitis. Human gingival fibroblasts (HGFs) were exposed to Fusobacterium nucleatum in the presence and absence of ghrelin. GHS-R expression was determined by real-time PCR and immunocytochemistry. Furthermore, wound healing, cell viability, proliferation, and migration were evaluated. GHS-R expression was significantly higher at periodontitis sites as compared to healthy sites in rat tissues. F. nucleatum significantly increased the GHS-R expression and protein level in HGFs. Moreover, ghrelin significantly abrogated the stimulatory effects of F. nucleatum on CCL2 and IL-6 expressions in HGFs and did not affect cell viability and proliferation significantly. Ghrelin stimulated while F. nucleatum decreased wound closure, probably due to reduced cell migration. Our results show original evidence that bacterial infection upregulates GHS-R in rat periodontal tissues and HGFs. Moreover, our study shows that ghrelin inhibited the proinflammatory actions of F. nucleatum on HGFs without interfering with cell viability and proliferation, suggesting that ghrelin and its receptor may act as a protective molecule during bacterial infection on periodontal cells.
Atorvastatin Decreases Renal Calcium Oxalate Stone Deposits by Enhancing Renal Osteopontin Expression in Hyperoxaluric Stone-Forming Rats Fed a High-Fat Diet
Authors: Liu Chan Jung , Tsai Yau Sheng , Huang Ho Shiang
Published: March 2022

Calcium oxalate (CaOx) is the major constituent of kidney stones. Growing evidence shows a close connection between hyperlipidemia, cardiovascular disease (CVD), and the formation of kidney stones. Owing to their antioxidant properties, statins control hyperlipidemia and may ameliorate CaOx stone formation. The present study was designed to investigate the suppressive effects of statins on CaOx urolithiasis and their potential mechanism. We used rats fed a high-fat diet (HFD) to achieve hyperlipidemia (HL) and hydroxyproline (HP) water to establish a hyperoxaluric CaOx nephrolithiasis model; the animals were administered statins (A) for 28 days. The rats were divided into eight groups treated or not with A, i.e., Control, HP, HL, HL + HP. HL aggravated urinary calcium crystallization compared to the control. Due to increased expression of renal osteopontin (OPN), a key anti-lithic protein, and reduced free radical production, the calcium crystals in the urinary bladder increased as renal calcium deposition decreased. The levels of the ion activity product of CaOx (AP(CaOx)) decreased after statins administration, and AP(Calcium phosphate) (CaP) increased, which suggested the dominant calcium crystal composition changed from CaOx to CaP after statin administration. In conclusion, atorvastatin decreases renal CaOx stone deposits by restoring OPN expression in hyperoxaluric rats fed a HFD.
Prediction of the Neurotoxic Potential of Chemicals Based on Modelling of Molecular Initiating Events Upstream of the Adverse Outcome Pathways of (Developmental) Neurotoxicity
Authors: Gadaleta Domenico , Spînu Nicoleta , Roncaglioni Alessandra , Cronin Mark T. D. , Benfenati Emilio
Published: March 2022

Developmental and adult/ageing neurotoxicity is an area needing alternative methods for chemical risk assessment. The formulation of a strategy to screen large numbers of chemicals is highly relevant due to potential exposure to compounds that may have long-term adverse health consequences on the nervous system, leading to neurodegeneration. Adverse Outcome Pathways (AOPs) provide information on relevant molecular initiating events (MIEs) and key events (KEs) that could inform the development of computational alternatives for these complex effects. We propose a screening method integrating multiple Quantitative Structure–Activity Relationship (QSAR) models. The MIEs of existing AOP networks of developmental and adult/ageing neurotoxicity were modelled to predict neurotoxicity. Random Forests were used to model each MIE. Predictions returned by single models were integrated and evaluated for their capability to predict neurotoxicity. Specifically, MIE predictions were used within various types of classifiers and compared with other reference standards (chemical descriptors and structural fingerprints) to benchmark their predictive capability. Overall, classifiers based on MIE predictions returned predictive performances comparable to those based on chemical descriptors and structural fingerprints. The integrated computational approach described here will be beneficial for large-scale screening and prioritisation of chemicals as a function of their potential to cause long-term neurotoxic effects.
PAP8/pTAC6 Is Part of a Nuclear Protein Complex and Displays RNA Recognition Motifs of Viral Origin
Authors: Chambon Louise , Gillet François-Xavier , Chieb Maha , Cobessi David , Pfannschmidt Thomas , Blanvillain Robert
Published: March 2022

Chloroplast biogenesis depends on a complex transcriptional program involving coordinated expression of plastid and nuclear genes. In particular, photosynthesis-associated plastid genes are expressed by the plastid-encoded polymerase (PEP) that undergoes a structural rearrangement during chloroplast formation. The prokaryotic-type core enzyme is rebuilt into a larger complex by the addition of nuclear-encoded PEP-associated proteins (PAP1 to PAP12). Among the PAPs, some have been detected in the nucleus (PAP5 and PAP8), where they could serve a nuclear function required for efficient chloroplast biogenesis. Here, we detected PAP8 in a large nuclear subcomplex that may include other subunits of the plastid-encoded RNA polymerase. We have made use of PAP8 recombinant proteins in Arabidopsis thaliana to decouple its nucleus- and chloroplast-associated functions and found hypomorphic mutants pointing at essential amino acids. While the origin of the PAP8 gene remained elusive, we have found in its sequence a micro-homologous domain located within a large structural homology with a rhinoviral RNA-dependent RNA polymerase, highlighting potential RNA recognition motifs in PAP8. PAP8 in vitro RNA binding activity suggests that this domain is functional. Hence, we propose that the acquisition of PAPs may have occurred during evolution by different routes, including lateral gene transfer.
Non-Coding RNAs: New Biomarkers and Therapeutic Targets for Temporal Lobe Epilepsy
Authors: Manna Ida , Fortunato Francesco , De Benedittis Selene , Sammarra Ilaria , Bertoli Gloria , Labate Angelo , Gambardella Antonio
Published: March 2022

Temporal lobe epilepsy (TLE) is the most common form of focal epilepsy; it is considered a network disorder associated with structural changes. Incomplete knowledge of the pathological changes in TLE complicates a therapeutic approach; indeed, 30 to 50% of patients with TLE are refractory to drug treatment. Non-coding RNAs (ncRNAs), acting as epigenetic factors, participate in the regulation of the pathophysiological processes of epilepsy and are dysregulated during epileptogenesis. Abnormal expression of ncRNA is observed in patients with epilepsy and in animal models of epilepsy. Furthermore, ncRNAs could also be used as biomarkers for the diagnosis and prognosis of treatment response in epilepsy. In summary, ncRNAs can represent important mechanisms and targets for the modulation of brain excitability and can provide information on pathomechanisms, biomarkers and novel therapies for epilepsy. In this review, we summarize the latest research advances concerning mainly molecular mechanisms, regulated by ncRNA, such as synaptic plasticity, inflammation and apoptosis, already associated with the pathogenesis of TLE. Moreover, we discuss the role of ncRNAs, such as microRNAs, long non-coding RNAs and circular RNAs, in the pathophysiology of epilepsy, highlighting their use as potential biomarkers for future therapeutic approaches.
Time- and Frequency-Domain Analysis of Stroke Volume Variability Using Indoor Cycling to Evaluate Physical Load of Body
Authors: Lai Yu-Han , Lai Wei-Chen , Huang Po-Hsun , Hsiao Tzu-Chien
Published: March 2022

A potential myocardial injury can be induced by intensive sporting activities, which may be due to ventricular tachycardia or fibrillation when individuals continue to exercise during the maximum physical loading period (the aerobic capability plateau, ACP). Herein, we conducted an incremental exercise test with the RR-interval and SV-series measurements as the input and output of the circulatory system. Through time and frequency analyses, we aimed to identify the indicators for distinguishing the normal stage (S1), last stage before ACP (S2), and ACP stage (S3) during different incremental physical loads. The cross-correlation results of the RR interval and SV series showed that the maximum coefficient of S2 was significantly greater (p < 0.05) than that of S1 (median 0.91 to 0.87), and also significantly lower (p < 0.05) than that of S3 (median 0.87 to 0.60). The corresponding spectrum shows that the decreasing correlation coefficient of SVV and Heart rate variability can be used to assess whether the body has reached the ACP. These findings can be used as a guide for exercise healthcare. Pausing or reducing the exercise load before entering the ACP could effectively reduce the risk of myocardial injury.
Improving Variabilty Analysis through Scenario-Based Incompatibility Detection
Authors: Buccella Agustina , Pol’la Matías , Cechich Alejandra
Published: March 2022

Software Product Line (SPL) developments include Variability Management (VA) as a core activity aiming at minimizing the inherent complexity in commonality and variability manipulation. Particularly, the (automated) analysis of variability models refers to the activities, methods and techniques involved in the definition, design, and instantiation of variabilities modeled during SPL development. Steps of this analysis are defined as a variability analysis process (VA process), which is focused on assisting variability model designers in avoiding anomalies and/or inconsistencies, and minimizing problems when products are implemented and derived. Previously, we have proposed an approach for analyzing variability models through a well-defined VA process (named SeVaTax). This process includes a comprehensive set of scenarios, which allows a designer to detect (and even correct in some cases) different incompatibilities. In this work, we extend SeVaTax by classifying the scenarios according to their dependencies, and by assessing the use of these scenarios. This assessment introduces two experiments to evaluate accuracy and coverage. The former addresses responses when variability models are analyzed, and the latter the completeness of our process with respect to other proposals. Findings show that a more extensive set of scenarios might improve the possibilities of current practices in variability analysis.
Association of Subclinical Hypothyroidism with Present and Absent Anti-Thyroid Antibodies with PCOS Phenotypes and Metabolic Profile
Authors: Gawron Iwona Magdalena , Baran Rafał , Derbisz Kamil , Jach Robert
Published: March 2022

Background: Polycystic ovary syndrome (PCOS) and subclinical hypothyroidism (SCH) often coexist, but implications of the co-occurrence of two disorders have not yet been established. The objective is to conclude whether SCH with present or absent anti-thyroid antibodies (ATA) impacts on the PCOS phenotype and alters biochemical or clinical parameters. Methods: A retrospective cohort study was conducted in a tertiary reference center. Clinical and biochemical parameters of women with PCOS were analyzed. Results: A total of 367 women with PCOS were included in the study, 114 (31.1%) of whom were diagnosed with SCH and 16 (4.4%) with autoimmune thyroiditis (AIT). Among all parameters studied, the strongest relationship with SCH was confirmed for insulin resistance and dyslipidemia. SCH was an independent risk factor for insulin resistance. In SCH the additional presence of ATA did not exacerbate the metabolic disorders. There was no significant association of any PCOS phenotype with SCH, nor with the presence of circulating ATA. There was no significant difference in hormonal parameters and mFerriman–Gallwey scale score between women with PCOS with and without SCH. Conclusions: SCH alters metabolic, but not hormonal, parameters in PCOS. The diagnosis of SCH does not exclude the diagnosis of PCOS. The potential effect of positive ATA was insignificant.
The Role of Oral Antivirals for COVID-19 Treatment in Shaping the Pandemic Landscape
Authors: Anastassopoulou Cleo , Hatziantoniou Sophia , Boufidou Fotini , Patrinos George P. , Tsakris Athanasios
Published: March 2022

Several vaccines against coronavirus disease 2019 (COVID-19) were developed and made available in a record time, just over a year after the outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) [...]
Microsurgical Transplantation of Pedicled Muscles in an Isolation Chamber—A Novel Approach to Engineering Muscle Constructs via Perfusion-Decellularization
Authors: Cai Aijia , Zheng Zengming , Müller-Seubert Wibke , Biggemann Jonas , Fey Tobias , Beier Justus P. , Horch Raymund E. , Frieß Benjamin , Arkudas Andreas
Published: March 2022

Decellularized whole muscle constructs represent an ideal scaffold for muscle tissue engineering means as they retain the network and proteins of the extracellular matrix of skeletal muscle tissue. The presence of a vascular pedicle enables a more efficient perfusion-based decellularization protocol and allows for subsequent recellularization and transplantation of the muscle construct in vivo. The goal of this study was to create a baseline for transplantation of decellularized whole muscle constructs by establishing an animal model for investigating a complete native muscle isolated on its pedicle in terms of vascularization and functionality. The left medial gastrocnemius muscles of 5 male Lewis rats were prepared and raised from their beds for in situ muscle stimulation. The stimulation protocol included twitches, tetanic stimulation, fatigue testing, and stretching of the muscles. Peak force, maximum rate of contraction and relaxation, time to maximum contraction and relaxation, and maximum contraction and relaxation rate were determined. Afterwards, muscles were explanted and transplanted heterotopically in syngeneic rats in an isolation chamber by microvascular anastomosis. After 2 weeks, transplanted gastrocnemius muscles were exposed and stimulated again followed by intravascular perfusion with a contrast agent for µCT analysis. Muscle constructs were then paraffin embedded for immunohistological staining. Peak twitch and tetanic force values all decreased significantly after muscle transplantation while fatigue index and passive stretch properties did not differ between the two groups. Vascular analysis revealed retained perfused vessels most of which were in a smaller radius range of up to 20 µm and 45 µm. In this study, a novel rat model of heterotopic microvascular muscle transplantation in an isolation chamber was established. With the assessment of in situ muscle contraction properties as well as vessel distribution after 2 weeks of transplantation, this model serves as a base for future studies including the transplantation of perfusion-decellularized muscle constructs.
Total Wrist Arthroplasty—A Systematic Review of the Outcome, and an Introduction of FreeMove—An Approach to Improve TWA
Authors: Eschweiler Jörg , Li Jianzhang , Quack Valentin , Rath Björn , Baroncini Alice , Hildebrand Frank , Migliorini Filippo
Published: March 2022

The Swanson silicone prosthesis was one of the first devices to realize total wrist arthroplasty (TWA). It has been used regularly since the early 1960s. This systematic review of the literature evaluated the status quos of TWA. The present study was conducted according to the PRISMA guidelines. A literature search was made in Medline, PubMed, Google Scholar, and the Cochrane Library databases. The focus of the present study was on implant survivorship and related functional outcomes. Data from 2286 TWA (53 studies) were collected. Fifteen studies were included for the analysis of implant survivorship. Fifteen studies were included for the analysis of pain. Twenty-eight studies were included for the analysis of the Disabilities of the Arm, Shoulder, and Hand (DASH) score. Grip strength was tracked in 16 studies. The range of motion (RoM) was evaluated in 46 studies. For supination and pronation, 18 articles were available. Despite some methodological heterogeneities, TWA may be effective and safe in pain reduction and improving function and motion. There is still a range for a future improvement of the procedure.