bluuebunny/crossref_metadata_2025 huggingface dataset:
Cachexia as Evidence of the Mechanisms of Resistance and Tolerance during the Evolution of Cancer Disease
Authors: Maccio Antonio , Sanna Elisabetta , Neri Manuela , Oppi Sara , Madeddu Clelia
Published: March 2021

During its evolution, cancer induces changes in patients’ energy metabolism that strongly affect the overall clinical state and are responsible for cancer-related cachexia syndrome. To better understand the mechanisms underlying cachexia and its metabolic derangements, research efforts should focus on the events that are driven by the immune system activation during the evolution of neoplastic disease and on the phenomena of “resistance” and “tolerance” typically involved in the human body response against stress, pathogens, or cancer. Indeed, in the case where resistance is not able to eliminate the cancer, tolerance mechanisms can utilize the symptoms of cachexia (anemia, anorexia, and fatigue) to counteract unregulated cancer growth. These notions are also sustained by the evidence that cancer cachexia may be reversible if the resistance and tolerance phases are supported by appropriate antineoplastic treatments. Accordingly, there is no doubt that anticachectic therapies have an irreplaceable role in cases of reversible cancer cachexia where, if harmoniously associated with effective antineoplastic therapies, they can contribute to preserve the quality of life and improve prognosis. Such anticachectic treatments should be based on targeting the complex immunological, inflammatory, and metabolic pathways involved in the complex pathogenesis of cachexia. Meanwhile, the role of the anticachectic therapies is very different in the stage of irreversible cachexia when the available antineoplastic treatments are not able to control the disease and the resistance mechanisms fail with the prevalence of the tolerance phenomena. At this stage, they can be useful only to improve the quality of life, allowing the patient and their family to get a better awareness of the final phases of life, thereby opening to the best spiritual remodulation of the final event, death.
The Current Challenges for Drug Discovery in CNS Remyelination
Authors: Balestri Sonia , Del Giovane Alice , Sposato Carola , Ferrarelli Marta , Ragnini-Wilson Antonella
Published: March 2021

The myelin sheath wraps around axons, allowing saltatory currents to be transmitted along neurons. Several genetic, viral, or environmental factors can damage the central nervous system (CNS) myelin sheath during life. Unless the myelin sheath is repaired, these insults will lead to neurodegeneration. Remyelination occurs spontaneously upon myelin injury in healthy individuals but can fail in several demyelination pathologies or as a consequence of aging. Thus, pharmacological intervention that promotes CNS remyelination could have a major impact on patient’s lives by delaying or even preventing neurodegeneration. Drugs promoting CNS remyelination in animal models have been identified recently, mostly as a result of repurposing phenotypical screening campaigns that used novel oligodendrocyte cellular models. Although none of these have as yet arrived in the clinic, promising candidates are on the way. Many questions remain. Among the most relevant is the question if there is a time window when remyelination drugs should be administrated and why adult remyelination fails in many neurodegenerative pathologies. Moreover, a significant challenge in the field is how to reconstitute the oligodendrocyte/axon interaction environment representative of healthy as well as disease microenvironments in drug screening campaigns, so that drugs can be screened in the most appropriate disease-relevant conditions. Here we will provide an overview of how the field of in vitro models developed over recent years and recent biological findings about how oligodendrocytes mature after reactivation of their staminal niche. These data have posed novel questions and opened new views about how the adult brain is repaired after myelin injury and we will discuss how these new findings might change future drug screening campaigns for CNS regenerative drugs.
Ivabradine Induces Cardiac Protection against Myocardial Infarction by Preventing Cyclophilin-A Secretion in Pigs under Coronary Ischemia/Reperfusion
Authors: Hernandez Ignacio , Tesoro Laura , Ramirez-Carracedo Rafael , Diez-Mata Javier , Sanchez Sandra , Saura Marta , Zamorano Jose Luis , Zaragoza Carlos , Botana Laura
Published: March 2021

In response to cardiac ischemia/reperfusion, proteolysis mediated by extracellular matrix metalloproteinase inducer (EMMPRIN) and its secreted ligand cyclophilin-A (CyPA) significantly contributes to cardiac injury and necrosis. Here, we aimed to investigate if, in addition to the effect on the funny current (I(f)), Ivabradine may also play a role against cardiac necrosis by reducing EMMPRIN/CyPA-mediated cardiac inflammation. In a porcine model of cardiac ischemia/reperfusion (IR), we found that administration of 0.3 mg/kg Ivabradine significantly improved cardiac function and reduced cardiac necrosis by day 7 after IR, detecting a significant increase in cardiac CyPA in the necrotic compared to the risk areas, which was inversely correlated with the levels of circulating CyPA detected in plasma samples from the same subjects. In testing whether Ivabradine may regulate the levels of CyPA, no changes in tissue CyPA were found in healthy pigs treated with 0.3 mg/kg Ivabradine, but interestingly, when analyzing the complex EMMPRIN/CyPA, rather high glycosylated EMMPRIN, which is required for EMMPRIN-mediated matrix metalloproteinase (MMP) activation and increased CyPA bonding to low-glycosylated forms of EMMPRIN were detected by day 7 after IR in pigs treated with Ivabradine. To study the mechanism by which Ivabradine may prevent secretion of CyPA, we first found that Ivabradine was time-dependent in inhibiting co-localization of CyPA with the granule exocytosis marker vesicle-associated membrane protein 1 (VAMP1). However, Ivabradine had no effect on mRNA expression nor in the proteasome and lysosome degradation of CyPA. In conclusion, our results point toward CyPA, its ligand EMMPRIN, and the complex CyPA/EMMPRIN as important targets of Ivabradine in cardiac protection against IR.
The Vps13 Family of Lipid Transporters and Its Role at Membrane Contact Sites
Authors: Dziurdzik Samantha Katarzyna , Conibear Elizabeth
Published: March 2021

The conserved VPS13 proteins constitute a new family of lipid transporters at membrane contact sites. These large proteins are suspected to bridge membranes and form a direct channel for lipid transport between organelles. Mutations in the 4 human homologs (VPS13A–D) are associated with a number of neurological disorders, but little is known about their precise functions or the relevant contact sites affected in disease. In contrast, yeast has a single Vps13 protein which is recruited to multiple organelles and contact sites. The yeast model system has proved useful for studying the function of Vps13 at different organelles and identifying the localization determinants responsible for its membrane targeting. In this review we describe recent advances in our understanding of VPS13 proteins with a focus on yeast research.
Beyond Arabidopsis: BBX Regulators in Crop Plants
Authors: Talar Urszula , Kiełbowicz-Matuk Agnieszka
Published: March 2021

B-box proteins represent diverse zinc finger transcription factors and regulators forming large families in various plants. A unique domain structure defines them—besides the highly conserved B-box domains, some B-box (BBX) proteins also possess CCT domain and VP motif. Based on the presence of these specific domains, they are mostly classified into five structural groups. The particular members widely differ in structure and fulfill distinct functions in regulating plant growth and development, including seedling photomorphogenesis, the anthocyanins biosynthesis, photoperiodic regulation of flowering, and hormonal pathways. Several BBX proteins are additionally involved in biotic and abiotic stress response. Overexpression of some BBX genes stimulates various stress-related genes and enhanced tolerance to different stresses. Moreover, there is evidence of interplay between B-box and the circadian clock mechanism. This review highlights the role of BBX proteins as a part of a broad regulatory network in crop plants, considering their participation in development, physiology, defense, and environmental constraints. A description is also provided of how various BBX regulators involved in stress tolerance were applied in genetic engineering to obtain stress tolerance in transgenic crops.
Investigating T Cell Immunity in Cancer: Achievements and Prospects
Authors: Zeng Zhen , Chew Hui Yi , Cruz Jazmina G. , Leggatt Graham R. , Wells James W.
Published: March 2021

T cells play a key role in tumour surveillance, both identifying and eliminating transformed cells. However, as tumours become established they form their own suppressive microenvironments capable of shutting down T cell function, and allowing tumours to persist and grow. To further understand the tumour microenvironment, including the interplay between different immune cells and their role in anti-tumour immune responses, a number of studies from mouse models to clinical trials have been performed. In this review, we examine mechanisms utilized by tumour cells to reduce their visibility to CD8+ Cytotoxic T lymphocytes (CTL), as well as therapeutic strategies trialled to overcome these tumour-evasion mechanisms. Next, we summarize recent advances in approaches to enhance CAR T cell activity and persistence over the past 10 years, including bispecific CAR T cell design and early evidence of efficacy. Lastly, we examine mechanisms of T cell infiltration and tumour regression, and discuss the strengths and weaknesses of different strategies to investigate T cell function in murine tumour models.
A Review on Acoustic and Skid Resistance Solutions for Road Pavements
Authors: Copetti Callai Sergio , Sangiorgi Cesare
Published: March 2021

As cities grow in size, traffic also increases, thus making the population more exposed to road noise and traffic accidents. It is therefore important to study and understand which properties of the pavement influence its acoustic impact and skid resistance performance. The pavement texture plays a major role in generating noise and friction, and it can be engineered in order to control both of them at the same time. The phenomena regulating skid resistance are well understood today. The same applies for noise generation and propagation; the literature contains methods of designing the pavement surface layer to achieve consistent results. Several types of solutions can be found for asphalt mixtures, most of them derived from decades of studies and research. They use different approaches to be effective for noise and friction, but all have in common the control of the surface’s macro and microtexture. Finally, some considerations are made regarding novel paving solutions with artificial aggregates instead of natural ones to address noise and skid resistance.
Anti-Collision Systems in Tunneling to Improve Effectiveness and Safety in a System-Quality Approach: A Review of the State of the Art
Authors: Patrucco Mario , Pira Enrico , Pentimalli Salvatore , Nebbia Rebecca , Sorlini Achille
Published: March 2021

Tunnelling and underground construction operations are often characterized by critical safety issues mainly due to poor visibility and blind spots around large vehicles and equipment. This can lead to collisions between vehicles or between vehicles and pedestrians or structural elements, causing accidents and fatalities. To improve the OS&H conditions, it is important to investigate the possible introduction of innovative techniques and technologies to reduce the occurrences and consequences of shared spaces (spaces used by both vehicles and pedestrians). For this reason, research was conducted to investigate the possible use of different technologies of anti-collision systems in tunnelling operations. First, to achieve this goal, an extensive review of the literature was carried out in accordance with the PRISMA statement to select the current techniques and technologies used by general anti-collision systems in civil and mining construction sites. Then, the operating principles, the relative advantages and disadvantages, combinations, and costs were examined for each of these. Eight types of systems and many examples of applications of anti-collision systems in underground environments were identified as a result of the analysis of the literature. Generally, it was noted that the anti-collision techniques available have found limited application in the excavation sites of underground civil works up to the present day, though the improvement in terms of safety and efficiency would be considerable.
Exposure of Helicoverpa armigera Larvae to Plant Volatile Organic Compounds Induces Cytochrome P450 Monooxygenases and Enhances Larval Tolerance to the Insecticide Methomyl
Authors: Wu Choufei , Ding Chaohui , Chen Shi , Wu Xiaoying , Zhang Liqin , Song Yuanyuan , Li Wu , Zeng Rensen
Published: March 2021

Plants release an array of volatile chemicals into the air to communicate with other organisms in the environment. Insect attack triggers emission of herbivore-induced plant volatiles (HIPVs). How insect herbivores use these odors to plan their detoxification systems is vital for insect adaptation to environmental xenobiotics. Here we show that the larvae of Helicoverpa armigera (Hübner), a broadly polyphagous lepidopteran herbivore, have the capacity to use plant volatiles as cues to upregulate multiple detoxification systems, including cytochrome P450 monooxygenases (P450s), for detoxification of insecticides. Olfactory exposure of the fifth instars to two terpene volatiles limonene and nerolidol, and two green-leaf volatiles 2-heptanone and cis-3-hexenyl acetate significantly reduced larval susceptibility to the insecticide methomyl. However, larval pretreatment with piperonyl butoxide (PBO), a known P450 inhibitor, neutralized the effects of volatile exposure. Furthermore, larval exposure to the four plant volatiles enhanced activities of P450 enzymes in midguts and fatbodies, and upregulated expression of CYP6B2, CYP6B6 and CYP6B7, P450s involved in detoxification of the insecticide. Larval exposure to 2-heptanone and limonene volatiles also enhanced activities of glutathione-s-transferase and carboxylesterase. Our findings suggest that olfactory exposure to HIPVs enhances larval insecticide tolerance via induction of detoxification P450s.
Givira ethela (Neumoegen and Dyar, 1893) (Lepidoptera: Cossidae), A Previously Unidentified Pest on Vitis vinifera (L.)
Authors: Scaccini Davide , Ruzzier Enrico , Daane Kent M.
Published: March 2021

Grape cultivation is a billion-dollar agricultural sector in California, where invasive or novel pest species can disrupt management practices. We report herein on a new pest associated with California vineyards, the carpentermoth Givira ethela (Neumoegen and Dyar, 1893). Rather than an invasive species, G. ethela appears to be a newly recognized wood-boring pest of Vitis vinifera (L.) in regions of California’s Central Valley, where its initial occurrence has been dated back to, at least, the beginning of the 2000s. The habitus of adult, genitalia and pupa is illustrated. Givira ethela distribution in California is updated including published records and new data. Carpentermoth galleries seem to facilitate the access of Planococcus ficus Signoret, 1875 to vine sap and protection from natural enemies, environmental stresses, and pesticide treatments. Notes on pest status, life history, monitoring practices, natural enemies, and management options on grapes are also discussed. Tools for the Integrated Pest Management of G. ethela should include the correct identification of the insect and its damage, a full understanding of its biology and ecology, the application of monitoring methods, and the identification of economic thresholds and injury levels.
The Pitfalls in the Path of Probabilistic Inference in Forensic Entomology: A Review
Authors: Moreau Gaétan
Published: March 2021

To bridge the gap between experimentation and the court of law, studies in forensic entomology and other forensic sciences have to comply with a set of experimental rules to generate probabilistic inference of quality. These rules are illustrated with successional studies of insects on a decomposing substrate as the main example. The approaches that have been used in the scientific literature to solve the issues associated with successional data are then reviewed. Lastly, some advice to scientific editors, reviewers and academic supervisors is provided to prevent the publication and eventual use in court of forensic studies using poor research methods and abusing statistical procedures
Effects of Aphicides on Pecan Aphids and Their Parasitoids in Pecan Orchards
Authors: Slusher Eddie K. , Cottrell Ted , Acebes-Doria Angelita L.
Published: March 2021

Aphids are important pests of pecans. Traditionally, insecticides have been the primary method of management. However, over-reliance and non-judicious use has led to resistance and damage to natural enemy populations. Therefore, frequent assessment of insecticides is necessary in order to monitor resistance development and non-target impacts. Aphicides, flonicamid, sulfoxaflor, and afidopyropen were assessed for their effects on pecan aphids and parasitoid, Aphelinus perpallidus, in a mature pecan orchard in 2019 and 2020. Post-application assessments were performed 7, 14, and 21 days post-application. Leaf samples from non-treated trees had greater aphid numbers than treated trees 7 days post-application with differences diminishing throughout the other two treatment periods in 2019. In 2020, aphid numbers were lower but leaf samples from non-treated trees had more aphids than treated trees 7 days post-application in the lower canopy. These differences again diminished 14 and 21 days post-application. There was no difference among treatments in number of parasitoid adults or mummies. These findings indicate that pecan growers have multiple potential options available for aphid management that do not negatively impact the primary pecan aphid parasitoid. Implications of the results on pecan aphid management are discussed.
Impact Damage Ascertainment in Composite Plates Using In-Situ Acoustic Emission Signal Signature Identification
Authors: James Robin , Joseph Roshan Prakash , Giurgiutiu Victor
Published: March 2021

Barely visible impact damage (BVID) due to low velocity impact events in composite aircraft structures are becoming prevalent. BVID can have an adverse effect on the strength and safety of the structure. During aircraft inspections it can be extremely difficult to visually detect BVID. Moreover, it is also a challenge to ascertain if the BVID has in-fact caused internal damage to the structure or not. This paper describes a method to ascertain whether or not internal damage happened during the impact event by analyzing the high-frequency information contained in the recorded acoustic emission signal signature. Multiple 2 mm quasi-isotropic carbon fiber reinforced polymer (CFRP) composite coupons were impacted using the ASTM D7136 standard in a drop weight impact testing machine to determine the mass, height and energy parameters to obtain approximately 1” impact damage size in the coupons iteratively. For subsequent impact tests, four piezoelectric wafer active sensors (PWAS) were bonded at specific locations on each coupon to record the acoustic emission (AE) signals during the impact event using the MISTRAS micro-II digital AE system. Impact tests were conducted on these instrumented 2 mm coupons using previously calculated energies that would create either no damage or 1” impact damage in the coupons. The obtained AE waveforms and their frequency spectrums were analyzed to distinguish between different AE signatures. From the analysis of the recorded AE signals, it was verified if the structure had indeed been damaged due to the impact event or not. Using our proposed structural health monitoring technique, it could be possible to rapidly identify impact events that cause damage to the structure in real-time and distinguish them from impact events that do not cause damage to the structure. An invention disclosure describing our acoustic emission structural health monitoring technique has been filed and is in the process of becoming a provisional patent.
Analysis of Diagnostic Images of Artworks and Feature Extraction: Design of a Methodology
Authors: Amura Annamaria , Aldini Alessandro , Pagnotta Stefano , Salerno Emanuele , Tonazzini Anna , Triolo Paolo
Published: March 2021

Digital images represent the primary tool for diagnostics and documentation of the state of preservation of artifacts. Today the interpretive filters that allow one to characterize information and communicate it are extremely subjective. Our research goal is to study a quantitative analysis methodology to facilitate and semi-automate the recognition and polygonization of areas corresponding to the characteristics searched. To this end, several algorithms have been tested that allow for separating the characteristics and creating binary masks to be statistically analyzed and polygonized. Since our methodology aims to offer a conservator-restorer model to obtain useful graphic documentation in a short time that is usable for design and statistical purposes, this process has been implemented in a single Geographic Information Systems (GIS) application.
The Abundance and Diversity of Fungi in a Hypersaline Microbial Mat from Guerrero Negro, Baja California, México
Authors: Maza-Márquez Paula , Lee Michael D. , Bebout Brad M.
Published: March 2021

The abundance and diversity of fungi were evaluated in a hypersaline microbial mat from Guerrero Negro, México, using a combination of quantitative polymerase chain reaction (qPCR) amplification of domain-specific primers, and metagenomic sequencing. Seven different layers were analyzed in the mat (Layers 1–7) at single millimeter resolution (from the surface to 7 mm in depth). The number of copies of the 18S rRNA gene of fungi ranged between 106 and 107 copies per g mat, being two logarithmic units lower than of the 16S rRNA gene of bacteria. The abundance of 18S rRNA genes of fungi varied significantly among the layers with layers 2–5 mm from surface contained the highest numbers of copies. Fifty-six fungal taxa were identified by metagenomic sequencing, classified into three different phyla: Ascomycota, Basidiomycota and Microsporidia. The prevalent genera of fungi were Thermothelomyces, Pyricularia, Fusarium, Colletotrichum, Aspergillus, Botrytis, Candida and Neurospora. Genera of fungi identified in the mat were closely related to genera known to have saprotrophic and parasitic lifestyles, as well as genera related to human and plant pathogens and fungi able to perform denitrification. This research suggests that fungi in the mat may participate in nutrient recycling, modification of community composition through parasitic activities, and denitrification.
Estrogen Sulfotransferase (SULT1E1): Its Molecular Regulation, Polymorphisms, and Clinical Perspectives
Authors: Yi MyeongJin , Negishi Masahiko , Lee Su-Jun
Published: March 2021

Estrogen sulfotransferase (SULT1E1) is a phase II enzyme that sulfates estrogens to inactivate them and regulate their homeostasis. This enzyme is also involved in the sulfation of thyroid hormones and several marketed medicines. Though the profound action of SULT1E1 in molecular/pathological biology has been extensively studied, its genetic variants and functional studies have been comparatively rarely studied. Genetic variants of this gene are associated with some diseases, especially sex-hormone-related cancers. Comprehending the role and polymorphisms of SULT1E1 is crucial to developing and integrating its clinical relevance; therefore, this study gathered and reviewed various literature studies to outline several aspects of the function, molecular regulation, and polymorphisms of SULT1E1.
Differences in the Microbial Composition of Hemodialysis Patients Treated with and without β-Blockers
Authors: Lin Yi-Ting , Lin Ting-Yun , Hung Szu-Chun , Liu Po-Yu , Hung Wei-Chun , Tsai Wei-Chung , Tsai Yi-Chun , Delicano Rachel Ann , Chuang Yun-Shiuan , Kuo Mei-Chuan , Chiu Yi-Wen , Wu Ping-Hsun
Published: March 2021

β-blockers are commonly prescribed to treat cardiovascular disease in hemodialysis patients. Beyond the pharmacological effects, β-blockers have potential impacts on gut microbiota, but no study has investigated the effect in hemodialysis patients. Hence, we aim to investigate the gut microbiota composition difference between β-blocker users and nonusers in hemodialysis patients. Fecal samples collected from hemodialysis patients (83 β-blocker users and 110 nonusers) were determined by 16S ribosomal RNA amplification sequencing. Propensity score (PS) matching was performed to control confounders. The microbial composition differences were analyzed by the linear discriminant analysis effect size, random forest, and zero-inflated Gaussian fit model. The α-diversity (Simpson index) was greater in β-blocker users with a distinct β-diversity (Bray–Curtis Index) compared to nonusers in both full and PS-matched cohorts. There was a significant enrichment in the genus Flavonifractor in β-blocker users compared to nonusers in full and PS-matched cohorts. A similar finding was demonstrated in random forest analysis. In conclusion, hemodialysis patients using β-blockers had a different gut microbiota composition compared to nonusers. In particular, the Flavonifractor genus was increased with β-blocker treatment. Our findings highlight the impact of β-blockers on the gut microbiota in hemodialysis patients.
Predicting Risk of Antenatal Depression and Anxiety Using Multi-Layer Perceptrons and Support Vector Machines
Authors: Javed Fajar , Gilani Syed Omer , Latif Seemab , Waris Asim , Jamil Mohsin , Waqas Ahmed
Published: March 2021

Perinatal depression and anxiety are defined to be the mental health problems a woman faces during pregnancy, around childbirth, and after child delivery. While this often occurs in women and affects all family members including the infant, it can easily go undetected and underdiagnosed. The prevalence rates of antenatal depression and anxiety worldwide, especially in low-income countries, are extremely high. The wide majority suffers from mild to moderate depression with the risk of leading to impaired child–mother relationship and infant health, few women end up taking their own lives. Owing to high costs and non-availability of resources, it is almost impossible to diagnose every pregnant woman for depression/anxiety whereas under-detection can have a lasting impact on mother and child’s health. This work proposes a multi-layer perceptron based neural network (MLP-NN) classifier to predict the risk of depression and anxiety in pregnant women. We trained and evaluated our proposed system on a Pakistani dataset of 500 women in their antenatal period. ReliefF was used for feature selection before classifier training. Evaluation metrics such as accuracy, sensitivity, specificity, precision, F1 score, and area under the receiver operating characteristic curve were used to evaluate the performance of the trained model. Multilayer perceptron and support vector classifier achieved an area under the receiving operating characteristic curve of 88% and 80% for antenatal depression and 85% and 77% for antenatal anxiety, respectively. The system can be used as a facilitator for screening women during their routine visits in the hospital’s gynecology and obstetrics departments.
Surface Modification of Biomedical MgCa4.5 and MgCa4.5Gd0.5 Alloys by Micro-Arc Oxidation
Authors: Sakiewicz Piotr , Piotrowski Krzysztof , Bajorek Anna , Młynarek Katarzyna , Babilas Rafał , Simka Wojciech
Published: March 2021

The aim of this work was to characterize the structure and corrosion properties of the MgCa4.5(Gd0.5) alloys surface treated by the micro-arc oxidation (MAO) process. The MgCa4.5 and MgCa4.5Gd0.5 alloy samples were processed by MAO in an electrolyte composed of NaOH (10 g/dm3), NaF (10 g/dm3), NaH2PO4 (5 g/dm3), Na2SiO2·5H2O (10 g/dm3) and water. Two different voltages (120 V and 140 V) were used in the MAO process. The alloys protected by an oxide layer formed in the MAO were then the subject of corrosion resistance tests in an environment simulating the human body (Ringer’s solution). After the experiments, the resulting samples were investigated using SEM, XPS and EDS techniques. The addition of Gd affected the fragmentation of the coating structure, thereby increasing the specific surface; higher voltages during the MAO process increased the number and size of surface pores. Corrosion tests showed that the MgCa4.5Gd0.5 alloys were characterized by low polarization resistances and high corrosion current densities. The studies indicated the disadvantageous influence of gadolinium on the corrosion resistance of MgCa4.5 alloys. The immersion tests confirmed lower corrosion resistance of MgCa4.5Gd0.5 alloys compared to the referenced MgCa4.5 ones. The MgCa4.5 alloy with the MAO coating established at voltage 140 V demonstrated the best anticorrosion properties.
Studies of Resistance of PP/Natural Filler Polymer Composites to Decomposition Caused by Fungi
Authors: Włodarczyk-Fligier Anna , Polok-Rubiniec Magdalena
Published: March 2021

The article discusses the grain morphology of the natural filler from hazelnut and walnut shell flour. It was observed that the geometry of both meals is similar to each other and resembles uneven balls in shape. The heterogeneity and well-developed outer surface of the flour grains allow for filling the voids with the polymer matrix. The analysis of the surface of the SEM images allowed to observe the presence of natural filler flour grains in the entire volume of the produced polymer composites, uneven distribution and small agglomerates, as well as the presence of voids, distributed in the matrix and in the matrix/filler interface. As a result of the visual evaluation of the activity of microorganisms (mycelium) on the surface of the produced polymer composite materials PP/hazelnut and walnut shell flour with a different % share, different fraction, it was found that the best fungistatic effect was shown by the samples marked with the symbol hazelnut at the fraction 315–443 µm. The least fungistatic material was found to be the samples with walnut shell meal filler at the fraction 315–443 µm (F2 and F4), on which the microorganisms achieved significant growth (more than 50% of the test area). The highest value of contact angle was obtained for samples with hazelnut filler fraction 315–443 (C2 and C4), which also confirms its best fungistatic effect.
A Method for Evaluation the Fatigue Microcrack Propagation in Human Cortical Bone Using Differential X-ray Computed Tomography
Authors: Koudelka Petr , Kytyr Daniel , Fila Tomas , Sleichrt Jan , Rada Vaclav , Zlamal Petr , Benes Pavel , Bendova Vendula , Kumpova Ivana , Vopalensky Michal
Published: March 2021

Fatigue initiation and the propagation of microcracks in a cortical bone is an initial phase of damage development that may ultimately lead to the formation of macroscopic fractures and failure of the bone. In this work, a time-resolved high-resolution X-ray micro-computed tomography (CT) was performed to investigate the system of microcracks in a bone sample loaded by a simulated gait cycle. A low-cycle (1000 cycles) fatigue loading in compression with a 900 N peak amplitude and a 0.4 Hz frequency simulating the slow walk for the initialization of the internal damage of the bone was used. An in-house developed laboratory X-ray micro-CT imaging system coupled with a compact loading device were employed for the in situ uni-axial fatigue experiments reaching a μ2μm effective voxel size. To reach a comparable quality of the reconstructed 3D images with the SEM microscopy, projection-level corrections and focal spot drift correction were performed prior to the digital volume correlation and evaluation using differential tomography for the identification of the individual microcracks in the microstructure. The microcracks in the intact bone, the crack formation after loading, and the changes in the topology of the microcracks were identified on a volumetric basis in the microstructure of the bone.
Bottom-Up Development of Nanoimprinted PLLA Composite Films with Enhanced Antibacterial Properties for Smart Packaging Applications
Authors: Psochia Eleni , Papadopoulos Lazaros , Gkiliopoulos Dimitrios J. , Francone Achille , Grigora Maria-Eirini , Tzetzis Dimitrios , de Castro Joana Vieira , Neves Nuno M. , Triantafyllidis Konstantinos S. , Torres Clivia M. Sotomayor , Kehagias Nikolaos , Bikiaris Dimitrios N.
Published: March 2021

In this work, polymer nanocomposite films based on poly(L-lactic acid) (PLLA) were reinforced with mesoporous silica nanoparticles, mesoporous cellular foam (MCF) and Santa Barbara amorphous-15 (SBA). PLLA is a biobased aliphatic polyester, that possesses excellent thermomechanical properties, and has already been commercialized for packaging applications. The aim was to utilize nanoparticles that have already been established as nanocarriers to enhance the mechanical and thermal properties of PLLA. Since the introduction of antibacterial properties has become an emerging trend in packaging applications, to achieve an effective antimicrobial activity, micro/nano 3D micropillars decorated with cone- and needle-shaped nanostructures were implemented on the surface of the films by means of thermal nanoimprint lithography (t-NIL), a novel and feasible fabrication technique with multiple industrial applications. The materials were characterized regarding their composition and crystallinity using Fourier transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD), respectively, and their thermal properties using differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA). Their mechanical properties were examined by the nanoindentation technique, while the films’ antimicrobial activity against the bacteria Escherichia coli and Staphylococcus aureus strains was tested in vitro. The results demonstrated the successful production of nanocomposite PLLA films, which exhibited improved mechanical and thermal properties compared to the pristine material, as well as notable antibacterial activity, setting new groundwork for the potential development of biobased smart packaging materials.
Emergence of Self-Organized Dynamical Domains in a Ring of Coupled Population Oscillators
Authors: Rusakov Alexey V. , Tikhonov Dmitry A. , Nurieva Nailya I. , Medvinsky Alexander B.
Published: March 2021

We show that interactions of inherently chaotic oscillators can lead to coexistence of regular oscillatory regimes and chaotic oscillations in the rings of coupled oscillators provided that the level of interaction between the oscillators exceeds a threshold value. The transformation of the initially chaotic dynamics into the regular dynamics in a number of the coupled oscillators is shown to result from suppression of chaos by separation of certain oscillation periods from the continuous spectra, which are characteristic of chaotic oscillations.
Biocompatibility of the Biopolymer Cyanoflan for Applications in Skin Wound Healing
Authors: Costa Raquel , Costa Luís , Rodrigues Ilda , Meireles Catarina , Soares Raquel , Tamagnini Paula , Mota Rita
Published: March 2021

There is a great demand for the development of novel wound dressings to overcome the time and costs of wound care performed by a vast number of clinicians, especially in the current overburdened healthcare systems. In this study, Cyanoflan, a biopolymer secreted by a marine unicellular cyanobacterium, was evaluated as a potential biomaterial for wound healing. Cyanoflan effects on cell viability, apoptosis, and migration were assessed in vitro, while the effect on tissue regeneration and biosafety was evaluated in healthy Wistar rats. The cell viability and apoptosis of fibroblasts and endothelial cells was not influenced by the treatment with different concentrations of Cyanoflan, as observed by flow cytometry. Moreover, the presence of Cyanoflan did not affect cell motility and migratory capacity, nor did it induce reactive oxygen species production, even revealing an antioxidant behavior regarding the endothelial cells. Furthermore, the skin wound healing in vivo assay demonstrated that Cyanoflan perfectly adapted to the wound bed without inducing systemic or local oxidative or inflammatory reaction. Altogether, these results suggest that Cyanoflan is a promising biopolymer for the development of innovative applications to overcome the many challenges that still exist in skin wound healing.
Renal Morphology in Coronavirus Disease: A Literature Review
Authors: de Oliveira Patrick , Cunha Kaile , Neves Precil , Muniz Monique , Gatto Giuseppe , Salgado Filho Natalino , Guedes Felipe , Silva Gyl
Published: March 2021

Renal biopsy is useful to better understand the histological pattern of a lesion (glomerular, tubulointerstitial, and vascular) and the pathogenesis that leads to kidney failure. The potential impact of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) on the kidneys is still undetermined, and a variety of lesions are seen in the kidney tissue of coronavirus disease patients. This review is based on the morphological findings of patients described in case reports and a series of published cases. A search was conducted on MEDLINE and PubMed of case reports and case series of lesions in the presence of non-critical infection by SARS-CoV-2 published until 15/09/2020. We highlight the potential of the virus directly influencing the damage or the innate and adaptive immune response activating cytokine and procoagulant cascades, in addition to the genetic component triggering glomerular diseases, mainly collapsing focal segmental glomerulosclerosis, tubulointerstitial, and even vascular diseases. Kidney lesions caused by SARS-CoV-2 are frequent and have an impact on morbidity and mortality; thus, studies are needed to assess the morphological kidney changes and their mechanisms and may help define their spectrum and immediate or long-term impact.
Spontaneous and Induced Tumors in Germ-Free Animals: A General Review
Authors: Mishra Rajbardhan , Rajsiglová Lenka , Lukáč Pavol , Tenti Paolo , Šima Peter , Čaja Fabián , Vannucci Luca
Published: March 2021

Cancer, bacteria, and immunity relationships are much-debated topics in the last decade. Microbiome’s importance for metabolic and immunologic modulation of the organism adaptation and responses has become progressively evident, and models to study these relationships, especially about carcinogenesis, have acquired primary importance. The availability of germ-free (GF) animals, i.e., animals born and maintained under completely sterile conditions avoiding the microbiome development offers a unique tool to investigate the role that bacteria can have in carcinogenesis and tumor development. The comparison between GF animals with the conventional (CV) counterpart with microbiome can help to evidence conditions and mechanisms directly involving bacterial activities in the modulation of carcinogenesis processes. Here, we review the literature about spontaneous cancer and cancer modeling in GF animals since the early studies, trying to offer a practical overview on the argument.
Effect of Yuzu (Citrus junos) Seed Limonoids and Spermine on Intestinal Microbiota and Hypothalamic Tissue in the Sandhoff Disease Mouse Model
Authors: Minamisawa Mayumi , Suzumura Takuma , Bose Sudeep , Taniai Tetsuyuki , Kawai Gota , Suzuki Kyoko , Yamaguchi Akira , Yamanaka Shoji
Published: March 2021

The effect of limonoids and spermine (Spm) extracted from yuzu (Citrus junos) seeds on the gut and the brain in a mouse model with Sandhoff disease (SD) was investigated. Wild-type and SD mice were fed a normal diet, or a diet supplemented with limonoid, Spm, or limonoid + Spm for 14–18 weeks, and then 16S rRNA gene amplicon sequencing with extracted DNA from their feces was executed. For SD control mice, intestinal microbiota was mostly composed of Lactobacillus and linked to dysbiosis. For SD and wild-type mice fed with limonoids + Spm or limonoids alone, intestinal microbiota was rich in mucin-degrading bacteria, including Bacteroidetes, Verrucomicrobia, and Firmicutes, and displayed a higher production of short-chain fatty acids and immunoglobulin A. Additionally, SD mice fed with limonoids + Spm or limonoids alone had less inflammation in hypothalamic tissues and displayed a greater number of neurons. Administration of limonoids and/or Spm improved the proportions of beneficial intestinal microbiota to host health and reduced neuronal degeneration in SD mice. Yuzu seed limonoids and Spermine may help to maintain the homeostasis of intestinal microbiota and hypothalamic tissue in the SD mouse model.
Multiple Amine-Contained POSS-Functionalized Organosilica Membranes for Gas Separation
Authors: Ren Xiuxiu , Kanezashi Masakoto , Guo Meng , Xu Rong , Zhong Jing , Tsuru Toshinori
Published: March 2021

A new polyhedral oligomeric silsesquioxane (POSS) designed with eight –(CH2)3–NH–(CH2)2–NH2 groups (PNEN) at its apexes was used as nanocomposite uploading into 1,2-bis(triethoxysilyl)ethane (BTESE)-derived organosilica to prepare mixed matrix membranes (MMMs) for gas separation. The mixtures of BTESE-PNEN were uniform with particle size of around 31 nm, which is larger than that of pure BTESE sols. The characterization of thermogravimetric (TG) and gas permeance indicates good thermal stability. A similar amine-contained material of 3-aminopropyltriethoxysilane (APTES) was doped into BTESE to prepare hybrid membranes through a copolymerized strategy as comparison. The pore size of the BTESE-PNEN membrane evaluated through a modified gas-translation model was larger than that of the BTESE-APTES hybrid membrane at the same concentration of additions, which resulted in different separation performance. The low values of Ep(CO2)-Ep(N2) and Ep(N2) for the BTESE-PNEN membrane at a low concentration of PNEN were close to those of copolymerized BTESE-APTES-related hybrid membranes, which illustrates a potential CO2 separation performance by using a mixed matrix membrane strategy with multiple amine POSS as particles.
Comparative Study of Epoxy-CsH2PO4 Composite Electrolytes and Porous Metal Based Electrocatalysts for Solid Acid Electrochemical Cells
Authors: Navarrete Laura , Yoo Chung-Yul , Serra José Manuel
Published: March 2021

Electrochemical cells based on acid salts (CsH2PO4) have attracted great interest for intermediate temperature, due to the outstanding proton conductivity of acid salts. In this work, electrodes and electrolyte were optimized following different strategies. An epoxy resin was added to the CsH2PO4 material to enhance the mechanical properties of the electrolyte, achieving good conductivity, enhanced stability, and cyclability. The electrodes configuration was modified, and Ni sponge was selected as active support. The infiltration of different oxide nanoparticles was carried out to tailor the electrodes resistance by promoting the electrocatalyst activity of electrodes. The selection of a cell supported on the electrode and the addition of an epoxy resin enables the reduction of the electrolyte thickness without damaging the mechanical stability of the thinner electrolyte.
Development of an In Vitro Airway Epithelial–Endothelial Cell Culture Model on a Flexible Porous Poly(Trimethylene Carbonate) Membrane Based on Calu-3 Airway Epithelial Cells and Lung Microvascular Endothelial Cells
Authors: Pasman Thijs , Baptista Danielle , van Riet Sander , Truckenmüller Roman K. , Hiemstra Pieter S. , Rottier Robbert J. , Hamelmann Naomi M. , Paulusse Jos M. J. , Stamatialis Dimitrios , Poot André A.
Published: March 2021

Due to the continuing high impact of lung diseases on society and the emergence of new respiratory viruses, such as SARS-CoV-2, there is a great need for in vitro lung models that more accurately recapitulate the in vivo situation than current models based on lung epithelial cell cultures on stiff membranes. Therefore, we developed an in vitro airway epithelial–endothelial cell culture model based on Calu-3 human lung epithelial cells and human lung microvascular endothelial cells (LMVECs), cultured on opposite sides of flexible porous poly(trimethylene carbonate) (PTMC) membranes. Calu-3 cells, cultured for two weeks at an air–liquid interface (ALI), showed good expression of the tight junction (TJ) protein Zonula Occludens 1 (ZO-1). LMVECs cultured submerged for three weeks were CD31-positive, but the expression was diffuse and not localized at the cell membrane. Barrier functions of the Calu-3 cell cultures and the co-cultures with LMVECs were good, as determined by electrical resistance measurements and fluorescein isothiocyanate-dextran (FITC-dextran) permeability assays. Importantly, the Calu-3/LMVEC co-cultures showed better cell viability and barrier function than mono-cultures. Moreover, there was no evidence for epithelial- and endothelial-to-mesenchymal transition (EMT and EndoMT, respectively) based on staining for the mesenchymal markers vimentin and α-SMA, respectively. These results indicate the potential of this new airway epithelial–endothelial model for lung research. In addition, since the PTMC membrane is flexible, the model can be expanded by introducing cyclic stretch for enabling mechanical stimulation of the cells. Furthermore, the model can form the basis for biomimetic airway epithelial–endothelial and alveolar–endothelial models with primary lung epithelial cells.
Synergistic Effect of rhBMP-2 Protein and Nanotextured Titanium Alloy Surface to Improve Osteogenic Implant Properties
Authors: Mesa-Restrepo Andrea , Civantos Ana , Allain Jean , Patiño Edwin , Alzate Juan , Balcázar Norman , Montes Robinson , Pavón Juan , Rodríguez-Ortiz José , Torres Yadir
Published: March 2021

One of the major limitations during titanium (Ti) implant osseointegration is the poor cellular interactions at the biointerface. In the present study, the combined effect of recombinant human Bone Morphogenetic Protein-2 (rhBMP-2) and nanopatterned Ti6Al4V fabricated with Directed irradiation synthesis (DIS) is investigated in vitro. This environmentally-friendly plasma uses ions to create self-organized nanostructures on the surfaces. Nanocones (≈36.7 nm in DIS 80°) and thinner nanowalls (≈16.5 nm in DIS 60°) were fabricated depending on DIS incidence angle and observed via scanning electron microscopy. All samples have a similar crystalline structure and wettability, except for sandblasted/acid-etched (SLA) and acid-etched/anodized (Anodized) samples which are more hydrophilic. Biological results revealed that the viability and adhesion properties (vinculin expression and cell spreading) of DIS 80° with BMP-2 were similar to those polished with BMP-2, yet we observed more filopodia on DIS 80° (≈39 filopodia/cell) compared to the other samples (<30 filopodia/cell). BMP-2 increased alkaline phosphatase activity in all samples, tending to be higher in DIS 80°. Moreover, in the mineralization studies, DIS 80° with BMP-2 and Anodized with BMP-2 increased the formation of calcium deposits (>3.3 fold) compared to polished with BMP-2. Hence, this study shows there is a synergistic effect of BMP-2 and DIS surface modification in improving Ti biological properties which could be applied to Ti bone implants to treat bone disease.