bluuebunny/crossref_metadata_2025 huggingface dataset:
Torrefaction of Willow in Batch Reactor and Co-Firing of Torrefied Willow with Coal
Authors: Unyay Hilal , Piersa Piotr , Zabochnicka Magdalena , Romanowska-Duda Zdzisława , Kuryło Piotr , Kuligowski Ksawery , Kazimierski Paweł , Hutsol Taras , Dyjakon Arkadiusz , Wrzesińska-Jędrusiak Edyta , Obraniak Andrzej , Szufa Szymon
Published: December 2023

The torrefaction process represents a thermal conversion technique conducted at relatively low temperatures ranging between 200 to 300 °C. Its objective is to produce fuel with a higher energy density by decomposing the reactive portion of hemicellulose. In this study, the kinetics of mass loss during torrefaction were investigated for willow. The experiments were carried out under isothermal conditions using thermogravimetric analysis. Batch torrefaction reactor designs were conducted and explained in detail. Co-combustion of willow with hard coal (origin: Katowice mine) in different mass ratios (25% biomass + 75% coal, 50% biomass + 50% coal, and 75% biomass + 25% coal) was conducted in addition to raw biomass torrefaction. TG/MS analysis (a combination of thermogravimetric analysis with mass spectrometry analysis) was performed in the research. The optimal torrefaction conditions for willow were identified as an average temperature of 245 °C and a residence time of 14 min, resulting in the lowest mass loss (30.15%). However, it was noted that the composition of torgas, a by-product of torrefaction, presents challenges in providing a combustible gas with sufficient heat flux to meet the energy needs of the process. Prolonged residence times over 15 min and higher average temperatures above 250 °C lead to excessive energy losses from volatile torrefaction products, making them suboptimal for willow. On the other hand, the co-combustion of torrefied biomass with hard coal offers advantages in reduced sulfur emissions but can lead to increased NOx emissions when biomass with a higher nitrogen content is co-combusted in proportions exceeding 50% biomass. This paper summarizes findings related to optimizing torrefaction conditions, challenges in torgas composition, and the emissions implications of co-combustion.
Comparative Analysis and Optimal Operation of an On-Grid and Off-Grid Solar Photovoltaic-Based Electric Vehicle Charging Station
Authors: Dukpa Andu , Butrylo Boguslaw , Venkatesh Bala
Published: December 2023

One of the key strategies for decarbonization and green transportation is using electric vehicles (EVs). However, challenges like limited charging infrastructure, EV battery characteristics, and grid integration complexities persist. This study proposes a mixed-integer linear programming (MILP) approach to optimize a grid-connected solar PV-based commercial EV charging station (SPEVCS) with a battery energy storage system (BESS) for profit maximization. The MILP model efficiently manages SPEVCS operations, considering solar power fluctuations, EV charging patterns, and BESS usage. By coordinating charging schedules, grid stability is reinforced, and excess solar power can be lucratively managed. Comparing grid-connected and off-grid SPEVCS scenarios highlights grid integration benefits. Solar power mismatches with optimal charging periods pose a challenge, addressed here by BESS utilization and import/export of deficit/surplus power from/to the grid. The proposed framework incorporates solar power forecasts and probabilistic EV arrival predictions, enhancing decision accuracy. This approach fosters viable commercial EV charging, promotes green transportation, and reinforces grid resilience.
Chemometric Differentiation of White Wines from a Low-Aromatic Grape Obtained by Spontaneous Fermentation, Enriched with Non-Saccharomyces, or with a High-Glutathione-Producing Saccharomyces Yeast
Authors: Muñoz-Castells Raquel , Moreno Juan , García-Martínez Teresa , Mauricio Juan Carlos , Moreno-García Jaime
Published: December 2023

This work focuses on the establishment of analytical and sensory differences between young wines obtained from the same grape must fermented using different strategies. The main purpose is to provide winemakers with objective criteria to help them to choose the best fermentation method to obtain the desired wine characteristics. The effects of four strategies were tested: a spontaneous fermentation with wild yeasts (WYs) and the addition of starter cultures of Lachancea thermotolerans (LT), Metschnikowia pulcherrima (MP), and a Saccharomyces cerevisiae strain that is an overproducer of glutathione (SC) in different batches of the same must of the Pedro Ximénez white grape. The analytical results obtained show as the LT wine has the highest glutathione content, while the lowest ethanol concentrations. The use of chemometric tools applied to the volatile compounds allowed to differentiate the four wines by a principal component analysis (PCA) and the identification of 27 key compounds. The four wines did not show statistical differences in their smell or taste attributes and only the LT wine was visually differentiated from the rest.
Experimental and Numerical Study of the Trench Fire Spread Rule over a Sloped Uniform Fuel Bed: Rate of Fire Spread, Flame Morphology, and Heat Flux
Authors: Wang Yi , Huang Rui
Published: December 2023

Trench fires on sloped terrain are always complicated due to the corresponding flame dynamics and heat transfer mechanisms. Flame attachment may increase the rate of fire spread (ROS) by enlarging the heating area of unburned vegetation. In addition, variations in radiative and convective heat flux are of great importance to fire behavior characteristics. In this work, trench fire tests under different slopes (θ) and inclined sidewalls (A) were performed by numerical simulations based on the Lagrangian Particle Model (LPM) and Boundary Fuel Model (BFM) in the Fire Dynamics Simulator (FDS) and small-scale experiments, and the ROS, flame characteristics, and radiative/convective heat flux of the fire front are discussed in detail. The results indicate that the flame tends to adhere to the fuel bed with increasing slope angle and sidewall inclination. In particular, the flame becomes fully attached with a greater pressure difference than the buoyancy, which is caused by the unequal air entrainment between the front and behind the flame. When A = 90°, the critical slope angle of the flame adhesion (from slight tilt to full attachment) is identified as ~20°. The ROS (θ ≤ 15°) predicted by the BFM and LPM are closer to the small-scale experiments. The heat fluxes based on the experiments confirm the predominant mechanism of radiative heat transfer in trench fires at low slopes (θ ≤ 20°). Furthermore, convective heat transfer is more significant than radiative and becomes the main heating mechanism for θ ≥ 20°.
Modelling Polyphenol Extraction through Ultrasound-Assisted Extraction by Machine Learning in Olea europaea Leaves
Authors: Rodríguez-Fernández Raquel , Fernández-Gómez Ángela , Mejuto Juan C. , Astray Gonzalo
Published: December 2023

The study of the phenolic compounds present in olive leaves (Olea europaea) is of great interest due to their health benefits. In this research, different machine learning algorithms such as RF, SVM, and ANN, with temperature, time, and volume as input variables, were developed to model the extract yield and the total phenolic content (TPC) from experimental data reported in the literature. In terms of extract yield, the neural network-based ANNZ-L model presents the lowest root mean square error (RMSE) value in the validation phase (9.44 mg/g DL), which corresponds with a mean absolute percentage error (MAPE) of 3.7%. On the other hand, the best model to determine the TPC value was the neural network-based model ANNR, with an RMSE of 0.89 mg GAE/g DL in the validation phase (MAPE of 2.9%). Both models obtain, for the test phase, MAPE values of 4.9 and 3.5%, respectively. This affirms that ANN models would be good modelling tools to determine the extract yield and TPC value of the ultrasound-assisted extraction (UAE) process of olive leaves under different temperatures, times, and solvents.
Bioprospecting of a Metschnikowia pulcherrima Indigenous Strain for Chasselas Winemaking in 2022 Vintage
Authors: Sizzano Federico , Blackford Marie , Berthoud Hélène , Amiet Laurent , Bailly Sébastien , Vuichard Frédéric , Monnard Christine , Bieri Stefan , Spring Jean-Laurent , Barth Yannick , Descombes Corentin , Lefort François , Cléroux Marilyn , Simonin Scott , Chappuis Charles , Bourdin Gilles , Bach Benoît
Published: December 2023

Interest in Metschnikowia (M.) pulcherrima is growing in the world of winemaking. M. pulcherrima is used both to protect musts from microbial spoilage and to modulate the aromatic profile of wines. Here, we describe the isolation, characterization, and use of an autochthonous strain of M. pulcherrima in the vinification of Chasselas musts from the 2022 vintage. M. pulcherrima was used in co-fermentation with Saccharomyces cerevisiae at both laboratory and experimental cellar scales. Our results showed that M. pulcherrima does not ferment sugars but has high metabolic activity, as detected by flow cytometry. Furthermore, sensory analysis showed that M. pulcherrima contributed slightly to the aromatic profile when compared to the control vinifications. The overall results suggest that our bioprospecting strategy can guide the selection of microorganisms that can be effectively used in the winemaking process.
Inland Record of the Last Interglacial Maximum in the Western Mediterranean: Revealing the Aljezares Pleistocene Basin (Alicante, SE-Spain)
Authors: Cuevas-González Jaime , Díez-Canseco Davinia , Elez Javier , Pérez-Mejías Carlos , Cheng Hai , Cañaveras Juan Carlos
Published: December 2023

The search for a continuous continental record of interglacial periods in semi-arid regions is problematic due to the absence of stable and continuous sedimentary systems over time in this type of climate. In this work, a relatively stable basin is described and analyzed during the last interglacial period in a semi-arid region of the western Mediterranean. For this purpose, a geomorphological, stratigraphic and sedimentological study has been carried out, with dating through 230Th. A semi-endorheic Pleistocene section has been identified, with two units that correspond to a fluvial-lacustrine system (unit P1) and an alluvial system (unit P2). Unit P1 has been dated to the MIS 5e interglacial episode. A framework for future studies is described, in which the Aljezares Pleistocene basin can be considered as a possible source of paleoenvironmental and paleoclimatic information in semi-arid regions from the last interglacial period.
Monsters in Mirrors: Duality, Triangulation, and Multiplicity in Two Adaptations of Jekyll and Hyde
Authors: Mustafa Jamil
Published: December 2023

Jacques Derrida’s theory of deconstruction provides an ideal means of appreciating and interrogating the duality central to both Robert Louis Stevenson’s Strange Case of Dr Jekyll and Mr Hyde and its adaptations. Moreover, because deconstruction exposes binary oppositions as artificial and constrictive, it enables us to advance beyond them toward multiplicity, a term used by Gilles Deleuze for a complex, ever-changing, multipart structure that transcends unity. Roy Ward Baker’s Dr Jekyll and Sister Hyde (1971) and episodes of Showtime’s Penny Dreadful (2014–2016) offer fresh ways to think about—and beyond—the duality of culture’s most famously divided pair. The binary oppositions that organize each text are innovative, as are the ways in which these oppositions are reversed and conflated. Ultimately, these adaptations employ triangulation to deconstruct themselves, thereby demonstrating the limitations and instability of duality, as well as the possibilities of multiplicity.
Dating Violence and Mental Health in Emerging Adulthood
Authors: Matud M. Pilar , Hernández-Lorenzo D. Estefanía , Fortes Demelsa , Ibáñez Ignacio
Published: December 2023

Dating violence constitutes a serious social and health problem. This study aims to increase knowledge on dating violence in emerging adulthood by analysing the relevance of gender and of having or not having a current partner in the victimization and perpetration of such violence. It also analyses the association between dating violence and mental health, as well as the relevance of traditional gender role attitudes and the internalization of feminine/expressive and masculine/instrumental traits in the victimization and perpetration of such types of violence. The participants were 930 Spanish emerging adults who were assessed by six self-report questionnaires and scales. Men reported more psychological and physical violence victimization and physical violence perpetration than women, and women and men without a current partner reported more psychological and sexual violence than women and men with a current partner. Dating violence victimization was associated with more mental symptomatology, less life satisfaction, and lower self-esteem in men with a current partner and in women without a current partner. The main predictor of dating violence victimization was dating violence perpetration, and the main predictor of dating violence perpetration was victimization by such violence. More traditional gender role attitudes also predicted greater victimization and perpetration of dating violence, except among women without a current partner.
Nickel Ions Enhanced the Adaptability of Tomato Seedling Roots to Low-Nitrogen Stress by Improving Their Antioxidant Capacity
Authors: Ran Shengxiang , Zhang Kun , Zhou Yuqi , Huang Weiqun , Zhong Fenglin
Published: December 2023

To elucidate the physiological mechanisms underlying the impact of exogenous nickel ions (Ni2+) on the adaptability of tomato (Solanum lycopersicum L.) seedling roots to low-nitrogen levels, the cultivar ‘Micro Tom’ was selected as the experimental material and cultivated hydroponically in the cultivation room of the Fujian Agriculture and Forestry University. Two distinct nitrogen concentrations (7.66 and 0.383 mmol·L−1) and two different levels of Ni2+ (0 and 0.1 mg·L−1 of NiSO4·6H2O) were employed as treatments. On the 9th day of cultivation, we measured the root biomass, the concentrations of antioxidant compounds, and the activities of antioxidant enzymes in the tomato seedlings. The study showed that when the nitrogen levels were low, the growth and development of the tomato seedling roots were hindered. This led to a significant increase in the levels of hydrogen peroxide (H2O2), superoxide anion (O2−), and malondialdehyde (MDA), indicating oxidative damage to the roots. Conversely, treatment with Ni2+ induced a notable increase in the activity of antioxidant enzymes in the seedlings and augmented the accumulation of nonenzymatic antioxidants, such as ascorbic acid (ASA) and reduced glutathione (GSH), thereby enhancing the operational efficiency of the ascorbate–glutathione cycle (ASA–GSH). Consequently, this led to substantial reductions in the H2O2 and MDA levels, ultimately mitigating the oxidative damage inflicted on the tomato seedling roots subjected to low-nitrogen stress. In conclusion, exogenous Ni2+ can reduce the peroxidative damage of tomato seedlings by promoting antioxidase activity in tomato seedlings under low-nitrogen stress, improve the tolerance of tomato seedlings to low-nitrogen stress, and maintain the normal growth and development of tomato seedlings.
Tomato Accumulates Cadmium to a Concentration Independent of Plant Growth
Authors: Zhang Xingyu , Zhang Cong , Zhang Yuyang
Published: December 2023

Cadmium (Cd) contamination is a growing concern, as exposure to the metal has been shown to inhibit plant growth and development. However, soil Cd pollution in China is typically mild, and thus its concentration often does not impede plant growth. On the other hand, it is unknown if increased plant growth impacts Cd uptake, movement, and accumulation. Here, we analyzed the relationship between Cd accumulation in 31 tomato cultivars and the impact on specific growth parameters in mild Cd contamination. The results showed that there are variations in the Cd distribution among the 31 tomato cultivars studied. There were higher Cd concentrations in shoots of the cultivar ‘SV3557’, whereas root Cd concentrations were the lowest. The roots of the cultivar ‘HF11’ recorded the lowest Cd content but had higher Cd content in the shoots. The Cd concentration in roots and shoots was not related to root length, plant height, and root weight. However, Cd accumulation in the shoots was markedly promoted by root length and plant height, and Cd accumulation in the roots was promoted by root weight. Subsequently, we imposed Cd on four selected tomato cultivars to ascertain their accumulation in the shoot tissues. The results revealed that, among the four tomato cultivars, Cd was highly accumulated in the leaves, followed by the stems, and the fruits (leaf > stem > fruit). When identifying significant loci associated with Cd accumulation in tomato plants, it is crucial to find a suitable indicator to assess the plant’s ability to accumulate Cd. Thus, Cd concentration in shoots can be used as a reliable proxy for evaluating tomato plants’ capacity for Cd accumulation. This study serves as a valuable reference in guiding the selection of such an index.
Development of a Pilot Literacy Scale to Assess Knowledge, Attitudes, and Behaviors towards Climate Change and Infectious Disease Dynamics in Suriname
Authors: Matlack Meghan , Covert Hannah , Shankar Arti , Zijlmans Wilco , Abdoel Wahid Firoz , Hindori-Mohangoo Ashna , Lichtveld Maureen
Published: December 2023

Prior research has shown that climate literacy is sparse among low- and middle-income countries. Additionally, no standardized questionnaire exists for researchers to measure climate literacy among general populations, particularly with regards to climate change effects on vector-borne diseases (VBDs). We developed a comprehensive literacy scale to assess current knowledge, attitudes, and behaviors towards climate change and VBD dynamics among women enrolled in the Caribbean Consortium for Research in Environmental and Occupational Health (CCREOH) cohort in Suriname. Items were generated by our research team and reviewed by a group of six external climate and health experts. After the expert review, a total of 31 climate change and 21 infectious disease items were retained. We estimated our sample size at a 10:1 ratio of participants to items for each scale. In total, 301 women were surveyed. We validated our scales through exploratory (n = 180) and confirmatory factor analyses (n = 121). An exploratory factor analysis for our general Climate Change Scale provided a four-construct solution of 11 items. Our chi-squared value (X2 = 74.32; p = 0.136) indicated that four factors were sufficient. A confirmatory factor analysis reinforced our findings, providing a good model fit (X2 = 39.03; p = 0.23; RMSEA = 0.015). Our Infectious Disease Scale gave a four-construct solution of nine items (X2 = 153.86; p = 0.094). A confirmatory factor analysis confirmed these results, with a chi-squared value of 19.16 (p = 0.575) and an RMSEA of 0.00. This research is vitally important for furthering climate and health education, especially with increases in VBDs spread by Aedes mosquitoes in the Caribbean, South America, and parts of the southern United States.
Impact of the COVID-19 Pandemic on Physical Activity among Mostly Older, Overweight Black Women Living in the Rural Alabama Black Belt
Authors: Neal Whitney N. , Schleicher Erica A. , Baron Kerri , Oster Robert A. , Brown Nashira I. , Demark-Wahnefried Wendy , Pisu Maria , Baskin Monica L. , Parrish Kelsey B. , Cole William Walker , Thirumalai Mohanraj , Pekmezi Dori W.
Published: December 2023

Despite well-documented global declines in physical activity (PA) during the COVID-19 pandemic, little is known regarding the specific impact among underserved, rural Alabama counties. This is concerning as this region was already disproportionately burdened by inactivity and related chronic diseases and was among the hardest hit by COVID-19. Thus, the current study examined the effect of COVID-19 on PA in four rural Alabama counties. An ancillary survey was administered between March 2020 and August 2021 to the first cohort (N = 171) of participants enrolled in a larger PA trial. Main outcomes of this survey included the perceived impact of COVID-19 on PA, leisure-time PA, and social cognitive theory (SCT) constructs at 3 months. Almost half of the participants reported being less active during the pandemic (49.7%) and endorsed that COVID-19 made PA more difficult (47.4%), citing concerns such as getting sick from exercising outside of the home (70.4%) and discomfort wearing a face mask while exercising (58%). Perceived COVID-19 impact on PA was significantly associated with education, household dependents, and gender (p’s < 0.05). More women, parents, and college graduates reported that the COVID-19 pandemic made PA more difficult. Overall, there were no significant associations between PA, SCT constructs, or perceived COVID-19 impact on PA scores at 3 months. While the pandemic made PA difficult for many participants, these barriers were not associated with leisure-time PA levels or related theoretical mechanisms of action, which bodes well for the success of our ongoing intervention efforts and the resiliency of these communities.
Greatest Risk Factor for Death from COVID-19: Older Age, Chronic Disease Burden, or Place of Residence? Descriptive Analysis of Population-Level Canadian Data
Authors: Phillips Susan P. , Carver Lisa F.
Published: December 2023

During the first wave of COVID-19, three-quarters of Canadian deaths were among those age 80 and older. We examined whether age, chronic disease load, sex, or place was the strongest predictor of such deaths. A cross-sectional analysis of administrative data from 1 January 2020 to 30 October 2020 for the population of Ontario (n = 15,023,174) was performed. Using logistic regression analysis, we determined whether place of residence (community dwelling, community dwelling with formal home care, or long-term care facility), age group, sex, or chronic disease burden was most strongly associated with the outcome of death within 60 days of a positive SARS-CoV-2 PCR test. Overall, there were 2766 deaths attributed to COVID-19. The age-related odds of dying increased from 6.1 (age 65–74) to 13.4 (age 85 or older) relative to those aged <65 years. This age effect was dwarfed by an odds ratio of 117.1 for those living in long-term care versus independently in the community, adjusted for age, sex, and chronic disease burden. The risk of death from COVID-19 aligned much more with social realities than individual risks. The disproportionate mortality arising specifically from institutional residence demands action to identify sources and ameliorate the harms of living in such facilities.
Perspectives on Autism Spectrum Disorder Diagnosis, Symptoms, Treatment and Gender Roles: A Qualitative Study of Similarities and Differences between Sexes
Authors: Piening Sigrid , van Balkom Ingrid D. C. , Stapert Anne Fleur , Henning Daria , Greaves-Lord Kirstin , Davids Lineke R. C. D. , Castelein Stynke , Groen Yvonne
Published: December 2023

This study aims to compare the experiences of women and men of different age groups with regard to their first autism spectrum disorder (ASD) diagnosis, symptoms, treatment, and gender roles to inform our understanding in clinical practice of differences as well as similarities. Semi-structured interviews were conducted amongst 22 women (n = 12) and men (n = 10) in three adult age groups regarding their diagnostic process, symptoms, treatment, and gender roles. Participants also filled out questionnaires on gender traits, social support, coping, and quality of life. Framework analysis guidelines were followed to identify subthemes within the three pre-defined key themes of the semi-structured interviews, and quantitative analyses were performed on the questionnaire results. Women often had caregiver roles and were more focused on social and family-oriented life aspects than men. Family and societal expectations may have been different for women from an early age onward and were considered burdensome by some, but not all. Views on ASD diagnosis, symptoms, and treatment were largely individually determined. The questionnaire results mostly showed no significant sex differences. Perceived gender roles differed between participants. In diagnosis and treatment, awareness of general gender differences and gender roles is important, but inter-individual differences and similar experiences in men should not be overlooked.
Using Non-Violent Discipline Tools: Evidence Suggesting the Importance of Attunement
Authors: Quail Karen , Ward Catherine
Published: December 2023

Training in non-violent discipline is important to prevent violence against children and ensure that their caregivers remain a safe base for them. This paper aims to deepen understanding of non-violent discipline by exploring attunement as a mechanism in the effectiveness of non-violent discipline tools. Attunement describes the sensitive responsiveness of caregivers towards their children and has been found to be central to the formation of secure attachment bonds and development of self-regulation. It includes understanding or being “in tune with” the child’s needs and signals, matching these with appropriate responses. The objective of this paper is to explore attunement in relation to non-violent discipline. Peer-reviewed systematic reviews previously included in a systematic overview of evidence on non-violent discipline options were screened for information relevant to attunement. All reviews were published in English between 1999 and 2018 and offered evidence on at least one non-violent discipline tool. Although no reviews explicitly addressed attunement, evidence was found suggesting its importance in the use and effectiveness of discipline methods. Research directly investigating attunement in discipline is needed.
Climate Change Risks Disclosure: Do Business Strategy and Management Characteristics Matter?
Authors: Aldoseri Mahfod M. , Albaz Maged M.
Published: December 2023

This research aims to broaden the understanding of the determinants of climate change disclosure, where the study analyzes the impact of corporate business strategy and Chief Executive Officer (CEO) overconfidence on the level of climate change disclosure. The study followed a mixed-methods approach that combines quantitative and qualitative techniques to comprehensively examine the relationships used by the content analysis method to analyze the annual reports of a sample of Saudi companies for the period from 2019 to 2022 to measure the level of disclosure of practices related to climate change. The results of the study show that the companies that tend to adopt the initiative strategy provide more information about climate change than the defending companies do, while the CEO’s overconfidence does not affect the level of climate change disclosure. The results of the study indicate that the nature of the strategic direction adopted by the company is more important in determining the motives for disclosing climate change information than the personal characteristics of management.
Assessing the Phenotype of a Homologous Recombination Deficiency Using High Resolution Array-Based Comparative Genome Hybridization in Ovarian Cancer
Authors: Magadeeva Svetlana , Qian Xueqian , Korff Nadine , Flörkemeier Inken , Hedemann Nina , Rogmans Christoph , Forster Michael , Arnold Norbert , Maass Nicolai , Bauerschlag Dirk O. , Weimer Jörg P.
Published: December 2023

Ovarian cancer (OC) cells with homologous recombination deficiency (HRD) accumulate genomic scars (LST, TAI, and LOH) over a value of 42 in sum. PARP inhibitors can treat OC with HRD. The detection of HRD can be done directly by imaging these genomic scars, or indirectly by detecting mutations in the genes involved in HR. We show that HRD detection is also possible using high-resolution aCGH. A total of 30 OCs were analyzed retrospectively with high-resolution arrays as a test set and 19 OCs prospectively as a validation set. Mutation analysis was performed by HBOC TruRisk V2 panel to detect HR-relevant mutations. CNVs were clustered with respect to the involved HR genes versus the OC cases. In prospective validation, the HRD status determined by aCGH was compared with external HRD assessments. Two BRCA mutation carriers did not have HRD. OC could approximately differentiate into two groups with characteristic CNV patterns with different survival rates. Mutation frequencies have a linear regression on the HRD score. Mutations in individual HR-relevant genes do not always indicate HRD. This may depend on the mutation frequency in tumor cells. The aCGH shows the genomic scars of an HRD inexpensively and directly.
Hepatorenal Syndrome—Novel Insights into Diagnostics and Treatment
Authors: Badura Krzysztof , Frąk Weronika , Hajdys Joanna , Majchrowicz Gabriela , Młynarska Ewelina , Rysz Jacek , Franczyk Beata
Published: December 2023

Hepatorenal syndrome (HRS) is a disorder associated with cirrhosis and renal impairment, with portal hypertension as its major underlying cause. Moreover, HRS is the third most common cause of acute kidney injury, thus creating a major public health concern. This review summarizes the available information on the pathophysiological implications of HRS. We discuss pathogenesis associated with HRS. Mechanisms such as dysfunction of the circulatory system, bacterial infection, inflammation, impaired renal autoregulation, circulatory, and others, which have been identified as critical pathways for development of HRS, have become easier to diagnose in recent years. Additionally, relatively recently, renal dysfunction biomarkers have been found indicating renal injury, which are involved in the pathophysiology of HRS. This review also summarizes the available information on the management of HRS, focusing on vasoconstrictive drugs, renal replacement therapy, and liver transplant together with currently being investigated novel therapies. Analyzing new discoveries for the underlying causes of this condition assists the general research to improve understanding of the mechanism of pathophysiology and thus prevention of HRS.
Maize Dek407 Encodes the Nitrate Transporter 1.5 and Is Required for Kernel Development
Authors: Wang Hongqiu , Yan Xiaolan , Du Qingguo , Yan Pengshuai , Xi Jinjin , Meng Xiaoruo , Li Xuguang , Liu Huijian , Liu Guoqin , Fu Zhongjun , Tang Jihua , Li Wen-Xue
Published: December 2023

The kernel serves as the storage organ and harvestable component of maize, and it plays a crucial role in determining crop yield and quality. Understanding the molecular and genetic mechanisms of kernel development is of considerable importance for maize production. In this study, we obtained a mutant, which we designated defective kernel 407 (dek407), through ethyl methanesulfonate mutagenesis. The dek407 mutant exhibited reduced kernel size and kernel weight, as well as delayed grain filling compared with those of the wild type. Positional cloning and an allelism test revealed that Dek407 encodes a nitrate transporter 1/peptide transporter family (NPF) protein and is the allele of miniature 2 (mn2) that was responsible for a poorly filled defective kernel phenotype. A transcriptome analysis of the developing kernels showed that the mutation of Dek407 altered the expression of phytohormone-related genes, especially those genes associated with indole-3-acetic acid synthesis and signaling. Phytohormone measurements and analysis indicated that the endogenous indole-3-acetic acid content was significantly reduced by 66% in the dek407 kernels, which may be the primary cause of the defective phenotype. We further demonstrated that natural variation in Dek407 is associated with kernel weight and kernel size. Therefore, Dek407 is a potential target gene for improvement of maize yield.
Double Photocrosslinked Responsive Hydrogels Based on Hydroxypropyl Guar
Authors: Ospennikov Alexander S. , Shibaev Andrey V. , Philippova Olga E.
Published: December 2023

Double crosslinked hydrogels based on a biodegradable polymer were prepared by photocopolymerization of methacrylated hydroxypropyl guar (HPG-MA) and 3-acrylamidophenylboronic acid. Along with irreversible strong covalent crosslinks by methacrylic groups, these hydrogels contained labile boronate crosslinks formed as a result of the interaction of boronic acid with cis-diol moieties of HPG. These hydrogels demonstrated higher elasticity than HPG-MA hydrogels with only irreversible covalent crosslinks. Labile boronate crosslinks not only strengthened the hydrogels but also imparted to them pronounced responsive properties. It was demonstrated that the mechanical properties, the swelling behavior, as well as the uptake and release of some substances from the double crosslinked hydrogel were pH controlled. For instance, the hydrogels could release cationic disinfectant at a rate regulated by pH. Such photocrosslinkable in situ forming hydrogels are very promising for the production of smart coatings that release targeted substances at the desired rate.
A Stable Micellar Formulation of RAD001 for Intracerebroventricular Delivery and the Treatment of Alzheimer’s Disease and Other Neurological Disorders
Authors: Gianessi Laura , Magini Alessandro , Dominici Roberto , Giovagnoli Stefano , Dolcetta Diego
Published: December 2023

A large body of evidence, replicated in many mouse models of Alzheimer’s disease (AD), supports the therapeutic efficacy of the oral mammalian target of rapamycin inhibitors (mTOR-Is). Our preliminary data show that intracerebroventricular (ICV) administration of everolimus (RAD001) soon after clinical onset greatly diminished cognitive impairment and the intracellular beta amyloid and neurofibrillary tangle load. However, RAD001 shows >90% degradation after 7 days in solution at body temperature, thus hampering the development of proper therapeutic regimens for patients. To overcome such a drawback, we developed a stable, liquid formulation of mTOR-Is by loading RAD001 into distearoylphosphatidylethanolamine–polyethylene glycol 2000 (DSPE-PEG2000) micelles using the thin layer evaporation method. The formulation showed efficient encapsulation of RAD001 and a homogeneous colloidal size and stabilised RAD001, with over 95% of activity preserved after 14 days at 37 °C with a total decay only occurring after 98 days. RAD001-loaded DSPE-PEG2000 micelles were unchanged when stored at 4 and 25 °C over the time period investigated. The obtained formulation may represent a suitable platform for expedited clinical translation and effective therapeutic regimens in AD and other neurological diseases.
Design, Synthesis, and In Vitro and In Vivo Bioactivity Studies of Hydrazide–Hydrazones of 2,4-Dihydroxybenzoic Acid
Authors: Popiołek Łukasz , Gawrońska-Grzywacz Monika , Dziduch Aleksandra , Biernasiuk Anna , Piątkowska-Chmiel Iwona , Herbet Mariola
Published: December 2023

In this research, twenty-four hydrazide–hydrazones of 2,4-dihydroxybenzoic acid were designed, synthesized, and subjected to in vitro and in vivo bioactivity studies. The chemical structure of the obtained compounds was confirmed by spectral methods. Antimicrobial activity screening was performed against a panel of microorganisms for all synthesized hydrazide–hydrazones. The performed assays revealed the interesting antibacterial activity of a few substances against Gram-positive bacterial strains including MRSA—Staphylococcus aureus ATCC 43300 (compound 18: 2,4-dihydroxy-N-[(2-hydroxy-3,5-diiodophenyl)methylidene]benzohydrazide—Minimal Inhibitory Concentration, MIC = 3.91 µg/mL). In addition, we performed the in vitro screening of antiproliferative activity and also assessed the acute toxicity of six hydrazide–hydrazones. The following human cancer cell lines were used: 769-P, HepG2, H1563, and LN-229, and the viability of the cells was assessed using the MTT method. The HEK-293 cell line was used as a reference line. The toxicity was tested in vivo on Danio rerio embryos using the Fish Embryo Acute Toxicity (FET) test procedure according to OECD No. 236. The inhibitory concentration values obtained in the in vitro test showed that N-[(4-nitrophenyl)methylidene]-2,4-dihydroxybenzhydrazide (21) inhibited cancer cell proliferation the most, with an extremely low IC50 (Inhibitory Concentration) value, estimated at 0.77 µM for LN-229. In addition, each of the compounds tested was selective against cancer cell lines. The compounds with a nitrophenyl substituent were the most promising in terms of inhibition cancer cell proliferation. The toxicity against zebrafish embryos and larvae was also very low or moderate.
Local Effects of Steroid Hormones within the Bone Microenvironment
Authors: Sandor Luca F. , Ragacs Reka , Gyori David S.
Published: December 2023

Steroid hormone production via the adrenal cortex, gonads, and placenta (so-called glandular steroidogenesis) is responsible for the endocrine control of the body’s homeostasis and is organized by a feedback regulatory mechanism based on the hypothalamus–pituitary–steroidogenic gland axis. On the other hand, recently discovered extraglandular steroidogenesis occurring locally in different tissues is instead linked to paracrine or autocrine signaling, and it is independent of the control by the hypothalamus and pituitary glands. Bone cells, such as bone-forming osteoblasts, osteoblast-derived osteocytes, and bone-resorbing osteoclasts, respond to steroid hormones produced by both glandular and extraglandular steroidogenesis. Recently, new techniques to identify steroid hormones, as well as synthetic steroids and steroidogenesis inhibitors, have been introduced, which greatly empowered steroid hormone research. Based on recent literature and new advances in the field, here we review the local role of steroid hormones in regulating bone homeostasis and skeletal lesion formation. The novel idea of extraglandular steroidogenesis occurring within the skeletal system raises the possibility of the development of new therapies for the treatment of bone diseases.
Immortalized Canine Adipose-Derived Mesenchymal Stem Cells Maintain the Immunomodulatory Capacity of the Original Primary Cells
Authors: Yasumura Yuyo , Teshima Takahiro , Nagashima Tomokazu , Michishita Masaki , Takano Takashi , Taira Yoshiaki , Suzuki Ryohei , Matsumoto Hirotaka
Published: December 2023

Mesenchymal stem cells (MSCs) are a promising cell source for stem cell therapy of intractable diseases in veterinary medicine, but donor-dependent cellular heterogeneity is an issue that influences therapeutic efficacy. Thus, we previously established immortalized cells that maintain the fundamental properties of primary cells, but functional evaluation had not been performed. Therefore, we evaluated the immunomodulatory capacity of the immortalized canine adipose-derived MSCs (cADSCs) in vitro and in vivo to investigate whether they maintain primary cell functions. C57BL/6J mice were treated with dextran sulfate sodium (DSS) to induce colitis, injected intraperitoneally with immortalized or primary cADSCs on day 2 of DSS treatment, and observed for 10 days. Administration of immortalized cADSCs improved body weight loss and the disease activity index (DAI) in DSS-induced colitic mice by shifting peritoneal macrophage polarity from the M1 to M2 phenotype, suppressing T helper (Th) 1/Th17 cell responses and inducing regulatory T (Treg) cells. They also inhibited the proliferation of mouse and canine T cells in vitro. These immunomodulatory effects were comparable with primary cells. These results highlight the feasibility of our immortalized cADSCs as a cell source for stem cell therapy with stable therapeutic efficacy because they maintain the immunomodulatory capacity of primary cells.
Acceleration of Protein Degradation by 20S Proteasome-Binding Peptides Generated by In Vitro Artificial Evolution
Authors: Zhu Yunhao , Shigeyoshi Kaishin , Hayakawa Yumiko , Fujiwara Sae , Kishida Masamichi , Ohki Hitoshi , Horibe Tomohisa , Shionyu Masafumi , Mizukami Tamio , Hasegawa Makoto
Published: December 2023

Although the 20S core particle (CP) of the proteasome is an important component of the 26S holoenzyme, the stand-alone 20S CP acts directly on intrinsically disordered and oxidized/damaged proteins to degrade them in a ubiquitin-independent manner. It has been postulated that some structural features of substrate proteins are recognized by the 20S CP to promote substrate uptake, but the mechanism of substrate recognition has not been fully elucidated. In this study, we screened peptides that bind to the 20S CP from a random eight-residue pool of amino acid sequences using complementary DNA display an in vitro molecular evolution technique. The identified 20S CP-binding amino acid sequence was chemically synthesized and its effects on the 20S CP were investigated. The 20S CP-binding peptide stimulated the proteolytic activity of the inactive form of 20S CP. The peptide bound directly to one of the α-subunits, opening a gate for substrate entry on the α-ring. Furthermore, the attachment of this peptide sequence to α-synuclein enhanced its degradation by the 20S CP in vitro. In addition to these results, docking simulations indicated that this peptide binds to the top surface of the α-ring. These peptides could function as a key to control the opening of the α-ring gate.
CDKN1A/p21 in Breast Cancer: Part of the Problem, or Part of the Solution?
Authors: Manousakis Evangelos , Miralles Clàudia Martinez , Esquerda Maria Guimerà , Wright Roni H. G.
Published: December 2023

Cyclin-dependent kinase inhibitor 1A (Cip1/Waf1/CDKN1A/p21) is a well-established protein, primarily recognised for its pivotal role in the cell cycle, where it induces cell cycle arrest by inhibiting the activity of cyclin-dependent kinases (CDKs). Over the years, extensive research has shed light on various additional mechanisms involving CDKN1A/p21, implicating it in processes such as apoptosis, DNA damage response (DDR), and the regulation of stem cell fate. Interestingly, p21 can function either as an oncogene or as a tumour suppressor in these contexts. Complicating matters further, the expression of CDKN1A/p21 is elevated in certain tumour types while downregulated in others. In this comprehensive review, we provide an overview of the multifaceted functions of CDKN1A/p21, present clinical data pertaining to cancer patients, and delve into potential strategies for targeting CDKN1A/p21 as a therapeutic approach to cancer. Manipulating CDKN1A/p21 shows great promise for therapy given its involvement in multiple cancer hallmarks, such as sustained cell proliferation, the renewal of cancer stem cells (CSCs), epithelial–mesenchymal transition (EMT), cell migration, and resistance to chemotherapy. Given the dual role of CDKN1A/p21 in these processes, a more in-depth understanding of its specific mechanisms of action and its regulatory network is imperative to establishing successful therapeutic interventions.
BIRC5 Gene Polymorphisms Are Associated with a Higher Stage of Local and Regional Disease in Oral and Oropharyngeal Squamous Cell Carcinomas
Authors: Mumlek Ivan , Ozretić Petar , Sabol Maja , Leović Matko , Glavaš-Obrovac Ljubica , Leović Dinko , Musani Vesna
Published: December 2023

Oral squamous cell carcinoma (OSCC) and oropharyngeal squamous cell carcinoma (OPSCC) are the most common types of cancers in the head and neck region (HNSCC). Despite very aggressive treatment modalities, the five-year survival rate has not changed for decades and is still around 60%. The search for potential specific biomarkers of aggressiveness or outcome indicators could be of great benefit in improving the treatment of these patients. One of the potential biomarkers is survivin, the protein product of the BIRC5 gene. In this study, we investigated the occurrence of BIRC5 gene polymorphisms in 48 patients with OSCC and OPSCC compared with healthy controls. A total of 18 polymorphisms were found, 11 of which occurred in HNSCC with a minor allele frequency (MAF) of more than 5%. Five polymorphisms (rs3764383, rs9904341, rs2071214, rs2239680, rs2661694) were significantly associated with tumor size, tumor stage, and advanced regional disease, but had no impact on survival.
Molecular Insights of New Psychoactive Substances (NPS) 2.0
Authors: Di Trana Annagiulia , Montana Angelo , Lo Faro Alfredo Fabrizio , Busardò Francesco Paolo , Pichini Simona
Published: December 2023

The New Psychoactive Substances (NPS) phenomenon represents an ever-changing global issue, with a number of new molecules entering the illicit market every year in response to international banning laws [...]
NopAA and NopD Signaling Association-Related Gene GmNAC27 Promotes Nodulation in Soybean (Glycine max)
Authors: Wang Yue , Jia Xiaoke , Li Yansong , Ma Shengnan , Ma Chao , Xin Dawei , Wang Jinhui , Chen Qingshan , Liu Chunyan
Published: December 2023

Rhizobia secrete effectors that are essential for the effective establishment of their symbiotic interactions with leguminous host plants. However, the signaling pathways governing rhizobial type III effectors have yet to be sufficiently characterized. In the present study, the type III effectors, NopAA and NopD, which perhaps have signaling pathway crosstalk in the regulation of plant defense responses, have been studied together for the first time during nodulation. Initial qRT-PCR experiments were used to explore the impact of NopAA and NopD on marker genes associated with symbiosis and defense responses. The effects of these effectors on nodulation were then assessed by generating bacteria in which both NopAA and NopD were mutated. RNA-sequencing analyses of soybean roots were further utilized to assess signaling crosstalk between NopAA and NopD. NopAA mutant and NopD mutant were both found to repress GmPR1, GmPR2, and GmPR5 expression in these roots. The two mutants also significantly reduced nodules dry weight and the number of nodules and infection threads, although these changes were not significantly different from those observed following inoculation with double-mutant (HH103ΩNopAA&NopD). NopAA and NopD co-mutant inoculation was primarily found to impact the plant–pathogen interaction pathway. Common differentially expressed genes (DEGs) associated with both NopAA and NopD were enriched in the plant–pathogen interaction, plant hormone signal transduction, and MAPK signaling pathways, and no further changes in these common DEGs were noted in response to inoculation with HH103ΩNopAA&NopD. Glyma.13G279900 (GmNAC27) was ultimately identified as being significantly upregulated in the context of HH103ΩNopAA&NopD inoculation, serving as a positive regulator of nodulation. These results provide new insight into the synergistic impact that specific effectors can have on the establishment of symbiosis and the responses of host plant proteins.