bluuebunny/crossref_metadata_2025 huggingface dataset:
Fetal liver CD34+ contain human immune and endothelial progenitors and mediate solid tumor rejection in NOG mice
Authors: Celhar Teja , Li Xinyi , Zhao Yunqian , Tay Hui Chien , Lee Andrea , Liew Hui Hua , Shepherdson Edwin Kunxiang , Rajarethinam Ravisankar , Fan Yiping , Mak Anselm , Chan Jerry Kok Yen , Singhal Amit , Takahashi Takeshi
Published: January 2024

Background
Transplantation of CD34+ hematopoietic stem and progenitor cells (HSPC) into immunodeficient mice is an established method to generate humanized mice harbouring a human immune system. Different sources and methods for CD34+ isolation have been employed by various research groups, resulting in customized models that are difficult to compare. A more detailed characterization of CD34+ isolates is needed for a better understanding of engraftable hematopoietic and potentially non-hematopoietic cells. Here we have performed a direct comparison of CD34+ isolated from cord blood (CB-CD34+) or fetal liver (FL-CD34+) and their engraftment into immunocompromised NOD/Shi-scid Il2rgnull (NOG) mice.
Methods
Three separate cohorts of NOG mice were transplanted with either CB-CD34+, FL-CD34+ or FL-CD34+ depleted of CD14+ endothelial cells to generate CB-NOG, FL-NOG and FL-CD14−-NOG, respectively. After 15–20 weeks, the mice were sacrificed and human immune cell reconstitution was assessed in blood, bone marrow, spleen, lungs and liver. Liver sections were pathologically assessed upon Haematoxylin and Eosin staining. To assess the capability of allogenic tumor rejection in CB- vs FL-reconstituted mice, animals were subcutaneously engrafted with an HLA-mismatched melanoma cell line. Tumor growth was assessed by calliper measurements and a Luminex-based assay was used to compare the cytokine/chemokine profiles.
Results
We show that CB-CD34+ are a uniform population of HSPC that reconstitute NOG mice more rapidly than FL-CD34+. In addition to HSPC, FL-CD34+ isolates contain non-hematopoietic CD14+ endothelial cells that enhance the engraftment of the human immune system in FL-CD34+-transplanted NOG (FL-NOG) mice. We demonstrate that these CD14+CD34+ cells are capable of reconstituting Factor VIII-producing liver sinusoidal endothelial cells (LSEC) in FL-NOG. However, CD14+CD34+ also contribute to hepatic sinusoidal dilatation and immune cell infiltration, which may culminate in a graft-versus-host disease (GVHD) pathology upon long-term engraftment. Finally, using an HLA-A mismatched CDX melanoma model, we show that FL-NOG, but not CB-NOG, can mount a graft-versus-tumor (GVT) response resulting in tumor rejection.
Conclusion
Our results highlight important phenotypical and functional differences between CB- and FL-NOG and reveal FL-NOG as a potential model to study hepatic sinusoidal dilatation and mechanisms of GVT.
Causal Relationships between IL-17C and Psoriasis and Atopic Dermatitis in European Populations: A Mendelian Randomization Analysis
Authors: Li Ya , Cai Jun , Chong Huimin
Published: January 2024

Psoriasis and atopic dermatitis are only two of the inflammatory diseases that have been linked to IL-17C. However, it is unclear if there is a genetic link between IL-17C and developing psoriasis and atopic dermatitis. In this study, the possible link between genetically predisposed IL-17C and the risk of psoriasis and atopic dermatitis was comprehensively investigated. There was no overlap in the participant populations between IL-17C and psoriasis and atopic dermatitis genome-wide association studies, which provide the genetic resources. Inverse variance weighting, often used as MR analysis, is the main method. To guarantee the accuracy of our findings, a number of sensitivity studies were carried out. MR analysis revealed that IL-17C was reported to increase the risk of psoriasis (OR = 1.15, 95%CI[1.02ཞ1.3], P = 0.02) in this study. At the same time, this study did not observe that IL-17C would increase the risk of atopic dermatitis (OR = 0.96, 95%CI [0.87ཞ1.08], P = 0.53). Under all sensitivity assessments, the results remained stable. There is evidence that IL-17C raises the risk of psoriasis, but there is no such rise in the risk of atopic dermatitis.
Respiratory outcomes of neonates born after previable premature rupture of membranes and treated with gentle ventilation
Authors: Brady Sinead , Martin Mary , Keerthy Divya , Liao Grace , Breslin Noelle , Parravicini Elvira
Published: January 2024

Objective: We aim to describe neonatal respiratory outcomes following previable preterm premature rupture of membranes(PPROM) when gentle ventilation is utilized. We also report maternal morbidity and mortality.
Study Design: This is a retrospective single center cohort study of infants delivered between 2016 and 2020 that included infants born at ≥23 weeks without major congenital anomaly after a pregnancy complicated with PPROM before 23-week gestation. Statistical analysis utilized unpaired Student’s t test or Mann-Whitney U test when appropriate.
Results: 35 infants from 33 pregnancies were included. 91.4% of infants survived until discharge and 12.1% developed Bronchopulmonary Dysplasia(BPD). Those who developed BPD had significantly lower amniotic fluid levels prior to delivery(p<0.05). There was no significant maternal morbidity or mortality in this cohort.
Conclusion: This cohort had high survival and low rates of respiratory morbidities. This suggests the use of gentle ventilation might be the optimal strategy for patients born after previable PPROM.
The Usability and User Experience of an Interactive E-Learning Platform to Empower Older Adults When Using Electronic Personal Health Records: An Online Intervention Study
Authors: Perotti Luis , Stamm Oskar , Dietrich Michael , Buchem Ilona , Müller-Werdan Ursula
Published: January 2024

Purpose:
Since the introduction of the electronic Personal Health Record (ePHR) in Germany in 2021, usage rates among those with statutory health insurance have remained below one percent. The patient-administered conception of the ePHR presents access barriers, particularly for older adults. This study aimed to evaluate an e-learning platform as an empowerment tool for ePHR usage, tailored toward older adults.
Methods:
In this explorative study, a micro-learning-based prototype platform for effective utilization of the ePHR was evaluated through both an online (n = 53) and a face-to-face (n = 6) intervention. The usability of the e-learning platform was measured using the System Usability Scale (SUS) and user experience with the User Experience Questionnaire (UEQ).
Results:
The mean rating in the SUS was 67.9 in the online group and 70.4 in the face-to-face group. The results of the UEQ in the online group, based solely on the subscale perspicuity, were above average compared to the benchmark. There was a strong positive correlation between usability and the perception of how learning content was presented in the online group (p = <.001, r(40) = .72). An investigation of the face-to-face group helped identify key usability challenges.
Conclusion:
The results of our study suggest that aspects of the target group-oriented design of the learning platform, in particular, still need to be improved. The insights gleaned from this study will contribute to the ongoing development of the learning platform in subsequent phases. Future studies will investigate the platform’s effectiveness in empowering older adults.
Romantic relationship status during the year after traumatic brain injury in an Ecuadorian sample
Authors: Mascialino Guido , Rodríguez-Lorenzana Alberto , Paz Clara , Perrin Paul B. , Watson Jack , Arango-Lasprilla Juan Carlos
Published: January 2024

Background
Traumatic brain injury (TBI) is a major cause of death and disability worldwide and often leads to long-lasting emotional, physical, and cognitive changes and results in reduced functioning across multiple domains. These changes often lead to strain in marital relationships, as the uninjured spouse grapples with adapting to changes in their partner.
Aims: The purpose of this study was to evaluate romantic relationship status after TBI at 6- and 12-months following injury and to identify predictor variables of these changes.
Methods
Ninety-seven TBI survivors were recruited while hospitalized at a tertiary care center in Quito, Ecuador, and followed at 6 and 12 months after their injury. Hierarchical linear modeling (HLM) was used to examine baseline predictors of linear romantic relationship probability trajectories across 6 and 12 months after injury. The final set of HLMs included each of the previously significant predictors from the first model, time, and interaction terms between time and the previously significant predictor.
Results
The first HLM showed that the probability of having a romantic relationship remained stable between 6 and 12 months after TBI. Individuals who were employed at baseline had higher romantic relationship probability trajectories than did those who had been unemployed. Older individuals had higher romantic relationship probability trajectories than younger individuals did, and women had higher romantic relationship probability trajectories than men did.
Conclusions
This is the first study to examine romantic relationship probability trajectories for an Ecuadorian adult population with TBI, and the data are highly valuable for understanding post-TBI outcomes in the region. These results can inform interventions and support systems to bolster marital resilience in the aftermath of TBI. Further research is warranted to explore the nuances of these relationships and to validate these findings in diverse populations.
Evaluation of Arbitrage Behavior of Electricity Selling Companies Based on Structural Entropy Weight Method
Authors: Wang Linfeng , Wu Xiangming , Ma Guozhen , Li Hangtian , li yanmei
Published: January 2024

The evaluation of arbitrage behavior of power selling companies is a multi-attribute decision-making problem. In the process of evaluation, the main method is to collect qualitative evaluation information from experts to determine the weight of indicators and evaluate accordingly. The research on power selling companies is of great significance for regional development. Based on the basic overview of the power market, appropriate evaluation indicators are selected, with the structural entropy weight method used to assign weights to each evaluation indicator. Combined with the operating mechanism of the power market, an evaluation model is designed for the trading behavior of power selling companies. Finally, a fuzzy comprehensive evaluation method is adopted to evaluate the model.
Alcohol consumption among Iranian population: STEPS survey, 2021
Authors: Hajebi Amirali , Nasserinejad Maryam , Rezaei Negar , Azadnajafabad Sina , Rashidi Mohammad-Mahdi , Ahmadi Naser , Ghasemi Erfan , Farzi Yosef , Yoosefi Moein , Djalalinia Shirin , Fattahi Nima , Rezaei Shahabeddin , Mehr Elmira Foroutan , Kazemi Ameneh , Haghshenas Rosa , Rezaee Kamyar , Rankohi Azadeh Momen Nia , Afsari Massomeh , Mahdavihezaveh Alireza , Jamshidi Hamidreza , Farzadfar Farshad
Published: January 2024

Alcohol production and consumption have been prohibited in Iran for over four decades, leading to a typical underestimation of its consumption. This study aimed to assess the prevalence of alcohol consumption, its associated factors, and estimate per capita alcohol consumption among Iran's adult population. In this population-based survey, 27,874 adults from across Iran were selected using systematic proportional to size cluster sampling. Alcohol consumption was evaluated through a modified Persian version of the STEPS questionnaires from previous studies, applied over different timespans. Per capita consumption was calculated using the quantity-frequency method, expressed in liters of pure alcohol. Adjusted odds ratios were reported for associates of alcohol consumption concerning metabolic risk factors, sociodemographic elements, and lifestyle variables. The prevalence of lifetime alcohol consumption was 6.9% (95% CI: 6.5–7.2) in the adult population, with a notable sex difference (males: 13.7% [95% CI: 13-14.4]; females: 1.4% [95% CI: 1.1–1.6]). The 12-month prevalence was 3.8% (95% CI: 3.6–4.1). For individuals aged 18 and older, the per capita alcohol consumption in Iran was 0.12 liters. Factors such as being a lifetime smoker, younger, wealthier, and having 7–12 years of education were significantly linked to higher alcohol consumption. Significant associations were also observed between alcohol consumption and obesity, history of heart attacks, and physical injuries. The study revealed a complex relationship between alcohol consumption, behavioral risk factors, and metabolic profiles. Consequently, immediate preventive measures tailored to each factor's association with alcohol use are recommended.
Association between alcohol consumption and the risk of incident chronic kidney disease in community-dwelling older adults: a Korean nationwide population-based senior cohort study
Authors: SUN IN O , Lee Hui-Seung , Lim Chi-Yeon , Bae Eunjin , Hyun Young Youl , Chung Sungjin , Kwon Soon Hyo , Cho Jang-Hee , Yoo Kyung Don , Park Woo Yeong , Kim Hyunsuk , Yu Byung Chul , Ko Gang-Jee , Yang Jae Won , Hwang Won Min , Song Sang Heon , Shin Sung Joon , Hong Yu Ah
Published: January 2024

Background
The relationship between alcohol consumption and kidney function decline is not well established, especially in older adults. This study aimed to investigate the effects of alcohol consumption on the risk of incident chronic kidney disease (CKD) in community-dwelling older adults.
Methods
Adults aged ≥ 65 years with an estimated glomerular filtration rate (eGFR) ≥ 60 mL/min/1.73 m2 and without proteinuria between 2009 and 2010 were recruited and followed through December 2018 from a Korean national population-based cohort. Alcohol consumption was categorised into non-, mild, moderate, and heavy drinking groups based on self-administered questionnaires. New-onset CKD was defined as an eGFR < 60 mL/min/1.73 m2.
Results
Of the total 122,319 subjects, the non-, mild, moderate, and heavy drinking groups were 99,091 (81.0%), 14,842 (12.1%), 4,257 (3.5%), and 4,139 (3.4%), respectively. During follow-up, 19,796 (20.0%), 4,636 (31.2%), 1,696 (39.8%), and 1,695 (41.0%) developed CKD in the non-, mild, moderate, and heavy drinking groups. Univariate Cox regression analyses showed a significantly increased risk of incident CKD in older adults in all drinking groups compared to non-drinkers (all P < 0.001). However, hazard ratios (HR) for developing CKD were 0.90 (95% confidence interval [CI] 0.87–0.94, P < 0.001) for mild, 0.89 (95% CI 0.84–0.95, P < 0.001) for moderate, and 0.93 (95% CI 0.88–0.99, P = 0.027) for heavy drinkers after fully adjusting for confounding variables. This inverse relationship between alcohol consumption and CKD risk was observed in males in all drinking groups, whereas in females it was found only in mild drinkers.
Conclusion
In the subgroup analysis, the beneficial effect of alcohol consumption on incident CKD was prominent among moderate drinkers aged 65–75 years, male mild drinkers aged ≥ 75 years, and female mild drinkers aged < 85 years. This study shows that alcohol consumption is inversely associated with the risk of incident CKD in older adults. Further studies are required to elucidate the effects of alcohol on kidney damage in older adults.
Exploration of the association between inflammatory cytokines and development of new-onset atrial fibrillation in critically ill patients: a retrospective cohort study
Authors: Derocher Heather , Muscedere John , Maslove David M. , Boyd J. Gordon , Day Andrew G. , Sibley Stephanie
Published: January 2024

BackgroundNew-onset atrial fibrillation (new AF) in critically ill patients is associated with increased morbidity and mortality. There has been evidence linking atrial fibrillation with inflammation in both the setting of chronic atrial fibrillation and critical illness. In this study we compare serum inflammatory marker levels in patients admitted to the intensive care unit (ICU) with no atrial fibrillation (AF), chronic AF, and new AF.MethodsA retrospective chart review was performed on 129 mechanically ventilated, critically ill patients from the PREVAIL trial. History of cardiovascular disease, previous AF, and development of new AF were documented. Inflammatory cytokines TNFα, IL-1b, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12, IL-13, IL-17, granulocyte-colony stimulating factor, granulocyte-macrophage colony-stimulating factor, interferon-γ, monocyte chemoattractant protein-1, and macrophage inflammatory protein-1b had been collected as part of the PREVAIL trial and were compared between three patient groups, those with no AF, chronic AF, and new AF.ResultsHigher APACHE II scores were found in patients with chronic AF (28.0±8.7) and new AF (27.1±6.2) compared to patients with no AF (23.0±7.2). No significant differences were found between groups in any inflammatory markers at baseline or on admission days 4 or 7.ConclusionWe found no clear association between the studied inflammatory markers and the development of new AF, although this may be related to the timing of sampling. Further study is required to better elucidate the relationship between inflammatory cytokines and the development of new AF in critically ill patients.
Effectiveness of osteopathic craniosacral techniques: A meta-analysis
Authors: Amendolara Alfred , Sheppert Alexander , Powers Ryan , Payne Andrew , Stacey Stephen , Sant David
Published: January 2024

Background
Craniosacral osteopathic manipulative medicine—also known as craniosacral therapy (CST)—is a widely taught component of osteopathic medical education. It is included in the standard curriculum of osteopathic medical schools, despite controversy surrounding its use. This paper seeks to systematically review and conduct a meta-analysis of randomized controlled trials assessing the clinical effectiveness of CST compared to standard care, sham treatment, or no treatment in adults and children.
Methods
A search of Embase, PubMed, and Scopus was conducted on 10/29/2023. There was no restriction placed on the date of publication. Additionally, a Google Scholar search was conducted to capture grey literature. Backward citation searching was also implemented. All randomized controlled trials employing CST for any clinical outcome were included. Studies not available in English as well as any studies that did not report adequate data for inclusion in the meta-analysis were excluded. Multiple reviewers were used to assess for inclusions, disagreements were settled by consensus. PRISMA guidelines were followed in the reporting of this meta-analysis. Cochrane’s Risk of Bias 2 tool was used to assess for risk of bias. All data were extracted by multiple independent observers. Effect sizes were calculated using a Hedge’s G value (standardized mean difference) and aggregated using random effects models.
Results
The primary study outcome was the effectiveness of CST for selected outcomes as applied to non-healthy adults or children and measured by standardized mean difference effect size. Twenty-four RCTs were included in the final meta-analysis with a total of 1,613 participants. When results were analyzed by primary outcome, no significant effects were found. When secondary outcomes were included, results showed that only Neonate health, structure (g = 0.66, 95% CI [0.30; 1.02], Prediction Interval [-0.73; 2.05]) and Pain, chronic somatic (g = 0.34, 95% CI [0.18; 0.50], Prediction Interval [-0.41; 1.09]) show reliable, statistically significant effect. However, wide prediction intervals and high bias limit the real-world implications of this finding.
Conclusions and Relevance:
CST did not demonstrate broad significance in this meta-analysis, suggesting limited usefulness in patient care for a wide range of indications.
Semi-passive pilot scale bioreactors metal(loid) removal performance response to seasonal freeze-thaw cycle
Authors: Desmau Morgane , Simister Rachel , Baldwin Susan A. , Nielsen Guillaume
Published: January 2024

Managing mine-contact water effectively and sustainably in (sub)arctic regions is crucial for expanding mining activities. The demand for cost-effective (semi-)passive water treatment that relies on natural chemical and biological processes and can withstand challenging weather conditions is increasing. This study investigated the ability of four pilot-scale bioreactors inoculated with locally sourced bacteria andaffected by a freeze-thaw cycle to effectively remove selenium and antimony. The bioreactors were operated at a Canadian subarctic mine for a year. Two duplicate bioreactors were installed in a heated shed maintained at 5°C over winter, while two other duplicates were installed outdoors and left to freeze. The removal rate of selenium and antimony was monitored weekly, while a genomic characterization of the microbial populations in the bioreactors was performed monthly. The bioreactors successfully removed selenium and antimony over the year, demonstrating their ability to manage freeze-thaw cycles. The overall percentage of selenium and antimony removal was similar in the outside and inside bioreactors, apart from the spring thawing period, when removal in the outdoors bioreactors was slightly lower. The dominant taxonomic groups of microbial populations were similar in all bioreactors, with slight variations observed in their relative abundance over time. The microbial population composition was consistent and re-established quickly after spring thaw in the outside bioreactors. This study demonstrated that the removal capacity of bioreactors inoculated with locally sourced bacteria was not largely affected by a freeze-thaw cycle, highlighting the strength of using local resources to design bioreactors in extreme climatic conditions.
Association between sleep disorders and physical activity in middle-aged Americans: Data from NHANES 2007-2018
Authors: Fei ZhiYing , Zhu XiaoYing , Shan QiDan , Wan FangYuan , Tu YingYing , Lv XiaoHeng
Published: January 2024

Background: Despite numerous studies on physical activity and sleep disorders, few have focused on physical activity and sleep disorders in middle-aged people who are particularly stressed. A restricted cubic web (RCS) technique was applied to determine whether there is a dose-response relationship between physical activity and the self-rated prevalence of sleep disorders in middle-aged adults.
Methods: This study analyzed 8880 middle-aged adults aged 40-65 years who participated in the National Health and Nutrition Examination Survey (NHANES) 2007-2018. Logistic regression was used to estimate the odds ratio (OR) and 95% confidence interval (CI) between physical activity and the risk of sleep disorders in middle-aged adults. Thereafter, the dose-response connection was examined using RCS.
Results: After adjusting for potential confounders, subjects with MET values in the first quartile (Q1) compared to subjects with MET values in the second, third, and fourth quartiles had odds ratios (OR) for sleep disturbance that were0.851(95% CI=0.745–0.973), 0.800 (95% CI = 0.698–0.917) and 0.780 (95% CI = 0.680–0.895) , respectively. RCS regression showed a non-linear association between physical activity and sleep disorders in middle-aged adults (non-linearity P=0.0382). Furthermore, the prevalence of sleep disorders in middle-aged adults decreased with increasing physical activity, reaching a minimum when weekly physical activity was around 166.27MET*h(OR=0.885, 95% CI=0.799–0.981).
Limitations：Self-reporting of sleep disorder and physical activity may be biased.
Conclusion: Our research demonstrates that physical activity was associated with a reduced risk of sleep disorders.
Timing and co-occurrence of symptoms prior to a diagnosis of light chain (AL) amyloidosis
Authors: D'Souza Anita , Singh Ashima , Szabo Aniko , Lian Qinghua , Pezzin Liliana , Sparapani Rodney
Published: January 2024

It is well-established that light chain (AL) amyloidosis patients have multi-organ involvement and are often diagnosed after a lag period of increasing symptoms. We leverage electronic health record (EHR) data from the TriNetX research network to describe the incidence, timing, and co-occurrence of precursor conditions of interests in a cohort of AL amyloidosis patients identified between October 2015-December 2020. Nineteen precursor diagnoses of interest representing features of AL amyloidosis were identified using ICD codes up to 36 months prior to AL amyloidosis diagnosis. Among 1,401 patients with at least 36 months of EHR data prior to AL amyloidosis diagnosis, 46% were females, 16% were non-Hispanic Black, and 6% were Hispanic. The median age was 71 (range, 21–91) years. The median number of precursor diagnoses was 5 with dyspnea and fatigue being the most prevalent. The time from the first occurrence of a precursor to AL diagnosis ranged from 3.2 to 21.4 months. Analyses of pairwise co-occurrence of specific diagnoses indicated a high association (Cole’s coefficient > 0.6) among the examined precursor diagnoses. These findings provide novel information about the timing and co-occurrence of key precursor conditions and could be used to develop algorithms for early identification of AL amyloidosis.
Core-periphery structure of a medicinal botanical system in Uruguay
Authors: Latorre Elena Castiñeira , Borthagaray Ana , Canavero Andrés
Published: January 2024

Background: Human evolution has granted upon individual’s cognitive mechanisms necessary for remembering experiences, vital for both survival and reproduction. These experiences manifest into cultural traits, influencing human culture, particularly in healthcare and maintenance. Studies regarding medicinal plants and treatments are integral to the study of the medical botanical system. Pharmacopeias highlight the prevalence of specific species widely used, aligning with the “consensus within diversity theory” in evolutionary ethnobiology.
Methods: This study applies network analysis on the botanical system of “Parque Regional Quebradas del Norte”, Rivera, Uruguay.
Results: Study results demonstrate a core-periphery structure, with a tightly interconnected core resistant to fragmentation, ensuring structural stability. This study showed the existence of peripheral nodes in the entire system, improving resilience against disturbances and increasing population's health.
Conclusion: The core species, renowned for their versatility and multiple medicinal uses, treating less severe ailments effectively. Additionally, core plants serve as prototypes for innovations. Their extinction poses a threat to the system's resilience. Conversely, peripheral plants, though vulnerable, offer possibilities for therapeutic innovations. In the face of environmental change, conservation efforts should prioritize species that are vulnerable to extinction, particularly within the core. Simultaneously, preserving knowledge associated with peripheral plants presents a bicultural conservation strategy, ensuring the botanical system's robustness amongst evolving ecological conditions.
Factors affecting self-perceived mental health in the general older population during the Covid19 pandemic: a cross-sectional study
Authors: Koppner Jenny , Lindelöf Ann , Iredahl Fredrik , Tevell Maxine , Nilsson Staffan , Thorsell Annika , Faresjö Åshild , Larsen Hanna Israelsson
Published: January 2024

Background
Mental health problems among older people are large public health concerns but often go unrecognized and undertreated. During Covid-19 several restrictions regarding social contacts were launched, primarily for the old. The objective of this study is to investigate which factors that had the main negative affect on mental health in the older population during the pandemic.
Method
A cross-sectional cohort study set in Swedish primary care during the pandemic years 2021–2022. The population constitutes of 70-80-years-old, N = 260. Instruments used are Geriatric depression scale 20 (GDS20); Hospital anxiety and depression scale (HADS), and Perceived stress scale 10 (PSS10). Sociodemography and risk factors are explored. Outcome measures are factors independently associated with decreased mental health. Analyses were performed for the group as a whole and with logistic regression models comparing individuals who stated they were mentally affected by the pandemic to individuals who stated they were not.
Results
Participants who stated they were mentally affected by the Covid − 19 pandemic reported significantly higher levels of anxiety (p < 0.001), depression (p < 0.001), and stress (p = 0.026) compared to those who stated they were not mentally affected. Explanatory regression models of up to 50% showed that following factors were prominent among individuals who experienced a decline in their mental health due to the Covid − 19 pandemic (n = 24); impaired social life (OR 16.35, p < 0.001, CI 4.17–16.05), change in physical activity (OR 5.76, p = 0.006, CI 1.67–19.87), perceived family situation (OR 30,36, p = 0,008, CI 2,41–382,75), mild/moderate and high anxiety (OR 3.9, p = 0.043, CI 1.37–24.33, OR 10,8, p = 0.011, CI 1.34–57.15 respectively), and female gender (OR 4.7, p = 0.05, CI 1.04–26.15).
Conclusion
Anxiety, family situation, social life and change in physical activity were the main factors influencing the 70-80-years-old’s self-perceived mental health during the Covid-19 pandemic. Long-term effects of social restrictions on mental health in the older population need to be further investigated.
Unveiling Sociotherapists' Coping Resources: Exploring Professional Experiences through Thematic Analysis
Authors: Jankowiak Barbara , Soroko Emilia , Pietrzak Sebastian , Wilk Maciej
Published: January 2024

Sociotherapy is a widely used form of psychological and pedagogical group help for children and adolescents in Poland. However, it is still unknown under this name, which causes its marginalization in scientific research. Knowing that sociotherapists face myriad challenges in working with individuals, groups, and systemic challenges, we decided to examine the coping strategies that led them to successful outcomes while working with adolescents. We conducted in-depth interviews with 22 sociotherapists (aged 28–60), asking them to share narratives of difficulties they overcame at work. We analyzed the resulting statements about the subjects' experience using reflective thematic analysis. We created the following themes, which reflect the structure of resources (themes), mobilized by the sociotherapists: 1) “I can rely on other professionals”; 2) “I am ready to work on and with myself”; 3) “I am ready to be a container for their feelings”; 4) “I need to take action!”; 5) “But I'm a sociotherapist, and I have a mission!”. Findings show that effective coping involves seeking support from professionals and acknowledging a personal growth in the work environment. Supervision and crisis intervention policies may become vital resources for sociotherapists. Broader access to societies can strengthen their professional role and foster a community-supported principle of good practice in sociotherapy.
Complete genomic sequence of a novel botourmiavirus infecting the fungus Phomopsis asparagi
Authors: Zhou Jingyi , Liu Shuang , Xu Yun , Yan Junhan , Xie Changping , Zheng Li , Chen Daipeng
Published: January 2024

Here, we report a novel ourmia-like mycovirus, named “Phomopsis asparagi magoulivirus 1” (PaMV1), derived from the phytopathogenic fungus Phomopsis asparagi. The complete genome sequence of PaMV1 consisted of a positive-sense single-stranded RNA (+ ssRNA) of 2,639 bp in length with a GC content of 57.13%, containing a single open reading frame (ORF) which encodes a putative RNA-dependent RNA polymerase (RdRp) of 686 amino acids with a molecular mass of 78.57 kDa. RdRp phylogeny indicates that PaMV1 grouped together with Diaporthe gulyae magoulivirus 1 (DgMV1) forming a distinct clade. The results of RdRp sequence homology comparisons and phylogenetic analysis suggested that PaMV1 is a new member of the genus Magoulivirus within the family Botourmiaviridae.
Effect of lidocaine on prevention of postoperative delirium in elderly patients with intrathecal anesthesia:A randomized, controlled, double-blind prospective study
Authors: Lin Longxiang , Li Yan , Zheng Hong , Pan Lezhou , Chen Mengmeng , Lian Chaohui , ShangGuan Wangning
Published: January 2024

Background
Earlier findings have shown that elderly individuals are at high risk for postoperative delirium. Although the pathogenesis of this disease is still under study, it has been suggested that the release and inhibition of inflammatory factors contribute to its pathogenesis. Lidocaine is considered to have outstanding anti-inflammatory properties. The aim of this study was to observe changes in cognitive function after intraoperative lidocaine pump placement in elderly patients who underwent hip replacement surgery after intrathecal anesthesia.
Methods
Patients aged 60 to 80 years were selected as study subjects and randomly divided into a lidocaine group and a saline group. The Mini Mental State Examination(MMSE) score was assessed on the day before surgery (T1) and the third day after surgery (T2) for comparison. Venous blood samples were taken on T1, T2 and the fifth day after surgery (T3) for the detection of inflammatory factors.
Results
Statistical analysis of the data showed that, compared with those in the lidocaine group, the saline group had a lower MMSE score at T2 (26.6 ± 1.5 vs. 28.0 ± 0.9, P < 0.01) and a greater concentration of the proinflammatory factor interleukin-6 (172.4 ± 85 vs. 129.4 ± 46, P = 0.018). In addition, the concentration of the S100β protein in the lidocaine group decreased after reaching a peak at T2, which was not evident in the saline group.
Conclusions
These findings suggest that lidocaine may protect the nervous system by reducing the release of proinflammatory factors rather than increasing the release of anti-inflammatory factors.
Trial registration
This study was registered at ChineseClinicalTrials.gov ChiCTR2000039540 (30/10/2020).
Clinical Challenging Cases series: Novel Options for Refractory Cancer Therapy-induced Thrombocytopenia（r-CTIT）
Authors: Hong Li-Li , Wen Qiang , Zhou Hai-Li , Wang Shou-Jun , Chen Kai-Li , Mu Jie , Shen Siyu , Zhuang Xiao-Fen , Wang Chun-Feng , Leng Ya-Chun , Wang Yong-Jun , Zhuang Hai-Feng
Published: January 2024

Purpose
Refractory cancer therapy-induced thrombocytopenia (r-CTIT) presents a life-threatening complication of tumor therapy, for which there is currently no established and effective long-term treatment.
Methods
Umbilical cord blood (UCB) is rich in hematopoietic stem cells with multi-lineage differentiation potential, while umbilical cord mesenchymal stem cells (UC-MSCs) have been shown to support megakaryocytopoiesis and regulate the bone marrow microenvironment. The components of cells have demonstrated great value in the treatment of hematopoietic failure diseases, especially in patients with r-CTIT.To investigate the impact of intravenous sequential infusion of UCB and UC-MSCs in patients with r-CTIT, we conducted a follow-up study.
Results
Among the 13 patients evaluated, the platelet response rate was 84.6% (11/13). Complete response (CR) was observed in 53.8% (7/13) of the patients, while partial response (PR) was seen in 84.6% (9/13) of the patients. The median time for platelet recovery in all patients was 53 days (range: 6-217 days). Our findings revealed that the infusion of UCB and UC-MSCs in r-CTIT patients has shown positive effects on erythroid and neutrophil levels, with an 84.6% erythroid response rate (11/13) and a 92.3% improvement in neutrophil count. Furthermore, no severe adverse reactions to the infusion were observed.
Conclusions
Based on our study, we can conclude that the sequential infusion of UCB and UC-MSCs can be a novel and promising therapeutic approach for r-CTIT patients.
Genetic and Molecular Correlates of Cortical Thickness Alterations in Adults with Obsessive-Compulsive Disorder: A Transcription-Neuroimaging Association Analysis
Authors: Hu Xiao , Zhang Da , Teng Chang , Xu Yin , Tian Lei , Cao Ping , Wang Xiao , Li Zong , Guan Cheng
Published: January 2024

Although numerous neuroimaging studies have shown neural alterations in individuals with obsessive-compulsive disorder (OCD), a psychiatric disorder characterised by intrusive cognitions and repetitive behaviours, the molecular mechanisms linking brain structural changes and gene expression remain poorly understood. By combining the Allen Human Brain Atlas dataset with neuroimaging data from the Meta-Analysis (ENIGMA) consortium and independent cohorts, this study performed partial least squares regression and enrichment analysis to probe the correlation between transcription and cortical thickness variation in adults with OCD. The cortical map of case–control differences in cortical thickness was spatially correlated with cortical expression of a weighted combination of genes enriched for neurobiologically relevant ontology terms, preferentially expressed across different cell types and cortical layers. These genes were specifically expressed in brain tissue, spanning nearly all cortical developmental stages. Protein-protein interaction analysis revealed that these genes coded a network of proteins encompassing several highly interactive hubs. The findings of this study bridge the gap between neural structure and transcriptome data in OCD, fostering an integrative understanding of the potential biological mechanisms.
The Impact of Urban Health Services on the Residency Intentions ofOlder Migrant Workers
Authors: Pang Zhi , Xin Yi , Wang Jinyang
Published: January 2024

Objective
Migrant workers play a crucial role in China's urbanization, yet many are reluctant to stay in the cities where they have worked hard in their old age. This study analyzes the impact of health service accessibility on the residency intentions of older migrant workers. Using the concept of equalization, it proposes new requirements for the health security system, which is significant for the future construction of new urbanization in China and the strategic deployment of the aging migrant population.
Methods
This study mainly uses the 2017 Migrant Population Dynamic Monitoring Survey data released by the National Health and Family Planning Commission. It describes and analyzes health services in terms of spatial accessibility, appropriateness, and availability using descriptive statistics and binary Logistic regression analysis.
Results
Establishing health records and receiving health education are the main factors of health services that influence the residency intentions of migrant workers.
Conclusion
Improving the level of medical and health services can further enhance the residency intentions of older migrant workers. Under the strategic layout of Healthy China and the equalization of medical and health services, it is necessary to continue examining and improving the health service conditions of migrant workers to overcome the current dilemma of "flowing without staying".
Clinical indicators and reproductive tract microbiota abnormalities indicate the occurrence of endometriosis
Authors: Li Xiaoqing , Chen Cong , Zheng Yuanyuan , Lin Wenjing , Zhang Hongping , Pan Qionghui
Published: January 2024

Endometriosis is an inflammation-associated disease, primarily but not always associated with abnormal immune system function and expression of immune factors. The microbiota of the female reproductive tract, including the vagina and cervix, plays a crucial role in health and disease. The immune dysregulation caused by the imbalance of reproductive tract microbiota may contribute to endometriosis. In this study, 35 women was recruited, including 17 women with endometriosis and 18 healthy women, while their general clinical data, cervical secretions and vaginal secretions were collected. High-throughput sequencing technology was performed to analyze the cervical and vaginal microbiota. We found that patients with endometriosis have unique vaginal and cervical microbiota. Romboutsia, Ruminococcus, Phascolarctobacterium, and Olsenella in the reproductive tract had significant positive correlation with the visual analogue scale index for endometriosis, while Mobiluncus displayed a significant negative correlation with the visual analogue scale index, and Lactobacillus showed a significant negative correlation with the thyroid peroxidase antibody index. These clinical and microbiological indicators might be associated with endometriosis, and this study has clinical significance for the detection and prevention of endometriosis.
Values and preferences towards the use of prophylactic low-molecular-weight heparin during pregnancy: A convergent mixed-methods secondary analysis of data from the Decision Analysis in SHared decision making for Thromboprophylaxis during Pregnancy (DASH-TOP) study
Authors: León‑García Montserrat , Humphries Brittany , Xie Feng , Gravholt Derek , Golembiewski Elizabeth , Eckman Mark H. , Bates Shannon M. , Hargraves Ian , Pelayo Irene , López Sandra Redondo , Caño Juan Antonio Millón , Alcántara Milagros A. Suito , D'Souza Rohan , Shehata Nadine , Jack Susan M , Guyatt Gordon , Perestelo‑Perez Lilisbeth , Alonso‑Coello Pablo
Published: January 2024

Background:
Venous thromboembolism (VTE) in pregnancy is a major cause of maternal morbidity and mortality, and the use of preventive low-molecular-weight heparin (LMWH) can be challenging. Clinical guidelines recommend eliciting pregnant individuals’ preferences towards the use of daily injections of LMWH and discussing the best option through a shared decision-making (SDM) approach. Our aim was to identify individuals’ preferences concerning each of the main clinical outcomes, and categorize attributes influencing the use of LMWH during pregnancy.
Methods:
Design: Convergent mixed-methods.
Participants: Pregnant women or those planning a pregnancy with VTE recurrence risk.
Intervention: A SDM intervention about thromboprophylaxis with LMWH in pregnancy.
Analysis: Quantitatively, we report preference scores assigned to each of the health states. Qualitatively, we categorized preference attributes using Burke’s pentad of motives framework: scene, agent, agency, act, and purpose. We use mixed-method convergent analysis to report findings using side-by-side comparison of concordance/discordance.
Results:
The least valued health state was to experience a pulmonary embolism (PE), followed by major obstetrical bleeding (MOB), deep vein thrombosis (DVT), and using daily injections of LMWH (valued as closest to a ‘healthy pregnancy’). Women's previous experiences, access to care (scene) and shared decision-making (agent) affected preferences. LMWH's benefits were noted, but substantial drawbacks described (agency). The main goal was avoiding the risk of VTE (purpose). Side-by-side comparisons revealed concordance between motives and DVT and PE health states. Discordance appeared between using daily injections of LMWH and agent- and agency motives and between MOB and the agency motive.
Conclusions:
Mixed-methods provide a nuanced understanding of women's LMWH preferences, by quantifying health states preferences and exploring attributes qualitatively. Incorporating both methods may improve patient-centered care around preference-sensitive decisions in thromboprophylaxis during pregnancy.
Not all MYC FISH probes are created equal
Authors: Dupuy Amanda K , Tripodi Joseph , Mizhiritskaya Valentina , Bezugly Mariya , Karolczuk Ewa , Najfeld Vesna
Published: January 2024

Background
The MYC gene plays a critical role in the diagnosis, prognosis, and treatment of hematological malignancies, including B-cell lymphomas, and both acute myeloid and lymphoid leukemia. The MYC fluorescence in situ hybridization (FISH) break-apart probe (BAP) is used in clinical testing to detect MYC gene rearrangements. Since MYC genomic abnormalities include various breakpoints and more than 35 translocation partner genes, false-negative results could significantly affect patient care. Due to the COVID-19 pandemia, our validated Abbott MYC BAP became unavailable in May 2023 after 20 years of clinical use. To ensure uninterrupted clinical testing, we validated MYC BAPs from three companies using three bone marrow samples with known MYC abnormalities.
Results
Two of the three tested probe sets from three manufacturers (CytoCell, MetaSystems, and Empire Genomics) showed concordant results with Abbott’s BAP for all samples, while one manufacturer’s MYC BAP (Empire Genomics) showed concordance in only 1 of the 3 samples. Using reference loci provided by each company’s probe map, we demonstrated that the discordant results were due to the proximity of the 5’ and 3’ probes, which prevented detection of the known MYC rearrangements, resulting in false-negative results.
Conclusions
Our findings reveal high-risk vulnerabilities in diagnostic testing when presuming equivalency between commercially available MYC BAPs. We recommend that clinical laboratories, in their initial validation process, include probes from multiple sources to account for supply chain disruptions. Additionally, laboratories should carefully compare probe designs when selecting probe manufacturers to ensure consistent and accurate detection of all frequent MYC genomic abnormalities.
WITHDRAWN: Disease modifying effect of HBS1L-MYB in HbE/β-thalassemia patients
Authors: Ferdous Jannatul , Tasnim Marzia , Mannoor Kaiissar , Qadri Firdausi , Hosen Md. Ismail , Shekhar Hossain Uddin
Published: January 2024

Thalassemias are a group of autosomal recessive disorder and the most common inherited disease worldwide with wide geographical variation in incidence. Fetal hemoglobin, hemoglobin is the main oxygen carrier protein in the human fetus. Hemoglobin F is found in fetal red blood cells. Elevated fetal hemoglobin (HbF) level is known to ameliorate the severity of HbE/β and β-thalassemia. This study aimed to investigate whether two commonly known HbF-associated SNPs (rs28384513 & rs4895441) in the HBS1L-MYB region are associated with HbF level and disease severity in Bangladeshi HbE/β- thalassemia patients. Blood samples were collected from a total of 160 participants (120 HbE/β- thalassemia patients and 40 healthy controls). Hematological analysis was performed using complete blood count (CBC) and capillary Hb electrophoresis. After genomic DNA extraction, real-time PCR-based high-resolution melting (HRM) for SNP detection targeting the HBS1L-MYB intergenic region was done. For rs-28384513 SNP the distribution of the major allele ‘TT’ and mutant allele ‘TG + GG’ were found to be 30% and 70%, respectively, while for rs-4895441, 79% of major allele ‘AA’ and 21% of minor allele ‘GG’ were observed in the patients. Patients carrying rs28384513 and rs4895441 SNPs had significantly higher HbF (1.29 ± 1.63 and 1.49 ± 1.7g/dl, respectively) compared to major allele ‘TT’ and ‘AA’ (0.87 ± 1.1 and 1.19 ± 1.65g/dl, respectively) with a p-value of 0.01 and 0.02, respectively. It has been detected that HbF levels in SNP-carrying patients significantly correlated with the higher transfusion interval (60 days, r = 0.38, P < 0.0001) and age of first transfusion (65 months, r = 0.26, p < 0.0028) in these patients. Further, non-transfusion-dependent patients had the highest HbF level (2.03 ± 2.05g/dl) compared to transfusion-dependent moderate (0.58 ± 0.78g/dl) and severe (0.84 ± 1.27g/dl) patients generating a significant p-value < 0.0001 in One-Way ANOVA test. The minor allele frequencies of rs28384513 (G) and rs4895441 (G) were found to be 0.43 and 0.11 respectively in the present study population. These findings suggest that SNPs of HBS1L-MYB may have a role in elevated HbF levels and ameliorating disease severity in terms of transfusion in HbE/β-thalassemia patients.