bluuebunny/crossref_metadata_2025 huggingface dataset:
Forest Soil Cation Dynamics and Increases in Carbon on the Allegheny Plateau, PA, USA Following a Period of Strongly Declining Acid Deposition
Authors: Bailey Scott W. , Long Robert P. , Horsley Stephen B.
Published: March 2021

Reductions in exchangeable calcium and magnesium and increase in exchangeable aluminum concentrations have been shown in soils impacted by acid deposition, including at four sites on the Allegheny Plateau, PA, USA, sampled in 1967 and 1997 during a period of peak deposition. We repeated sampling at these sites in 2017 to evaluate changes in soils during the more recent period when there has been a strong decline in acid deposition. The uppermost horizons, including the Oa and A horizons where humified organic matter transitions to mineral soil, were thicker, had higher concentrations of organic carbon and exchangeable calcium and magnesium, and lower concentrations of exchangeable aluminum in 2017 compared to 1997, approximating values measured in 1967. Below the Oa/A horizons, 2017 soil chemistry was more similar to the 1997 results, with some reduction of Ca in the recent measurements. These results suggest recovery of base cation–aluminum balance in surface horizons and may indicate a reduction of aluminum mobilization and increased efficiency of vegetation recycling of nutrients with decreased acid anion concentrations. These changes are consistent with a partial recovery from acid deposition. However, the increase in humified soil organic matter may also be affected by coincident increases in temperature and soil moisture.
Rainfall Alters Permafrost Soil Redox Conditions, but Meta-Omics Show Divergent Microbial Community Responses by Tundra Type in the Arctic
Authors: Romanowicz Karl J. , Crump Byron C. , Kling George W.
Published: March 2021

Soil anoxia is common in the annually thawed surface (‘active’) layer of permafrost soils, particularly when soils are saturated, and supports anaerobic microbial metabolism and methane (CH4) production. Rainfall contributes to soil saturation, but can also introduce oxygen, causing soil oxidation and altering anoxic conditions. We simulated a rainfall event in soil mesocosms from two dominant tundra types, tussock tundra and wet sedge tundra, to test the impacts of rainfall-induced soil oxidation on microbial communities and their metabolic capacity for anaerobic CH4 production and aerobic respiration following soil oxidation. In both types, rainfall increased total soil O2 concentration, but in tussock tundra there was a 2.5-fold greater increase in soil O2 compared to wet sedge tundra due to differences in soil drainage. Metagenomic and metatranscriptomic analyses found divergent microbial responses to rainfall between tundra types. Active microbial taxa in the tussock tundra community, including bacteria and fungi, responded to rainfall with a decline in gene expression for anaerobic metabolism and a concurrent increase in gene expression for cellular growth. In contrast, the wet sedge tundra community showed no significant changes in microbial gene expression from anaerobic metabolism, fermentation, or methanogenesis following rainfall, despite an initial increase in soil O2 concentration. These results suggest that rainfall induces soil oxidation and enhances aerobic microbial respiration in tussock tundra communities but may not accumulate or remain in wet sedge tundra soils long enough to induce a community-wide shift from anaerobic metabolism. Thus, rainfall may serve only to maintain saturated soil conditions that promote CH4 production in low-lying wet sedge tundra soils across the Arctic.
Economic Aspects of Shrinking Cities in Poland in the Context of Regional Sustainable Development
Authors: Wichowska Anna
Published: March 2021

Two trends are observed in contemporary cities around the world: whereas some urban areas develop rapidly and experience population growth, a steady population decline is noted in other cities. Demographic changes in urban areas are also accompanied by economic changes. These changes constitute a very serious challenge for sustainable regional growth. However, these problems have not been sufficiently investigated to date, including in Poland. The aim of this study was to identify shrinking cities in Poland and the phenomena that are related to the economic aspects of urban shrinkage in Poland. Empirical research relied on analysis of the population growth rate in Polish urban municipalities, and the phenomena related to the economic aspects of urban shrinkage were identified by multiple linear regression analysis. The period of research was 2003–2019. Thirty-three Polish cities experienced a steady population decline. The economic phenomena related to urban shrinkage included changes in own-source revenues, proportions of government transfers in municipal budgets, unemployment, migration, municipal spending on education, transport, communications, and social welfare. Population decline was not related to changes in the age-dependency ratio, public spending on housing, the number of companies, or the number of vacant homes in cities. The research results can be a source of important information for regional sustainable growth policies used not only in cities and regions in Poland, but also in other Central and Eastern European countries where this phenomenon occurs.
Invisible and Insecure in Rural America: Cultivating Dignity in Local Food Security Initiatives
Authors: Herrington Amy , Mix Tamara L.
Published: March 2021

The United States’ neoliberal approach to governance promotes structural inequalities that shape individuals’ sense of dignity. We employ qualitative in-depth interviews and ethnographic field study to examine dignity construction via daily experiences with food access and foodways. Situating our study within a rural Oklahoma community with high food insecurity rates, we ask: How does structural inequality impact individuals’ daily experiences with dignity construction? How is a sense of dignity influenced by daily experiences with food access and foodways within the context of community-based food initiatives? We address structural inequality and the resulting social hierarchy of food security, focusing on three overlapping social arenas—relational, individual, and institutional. Relational interactions in food access spaces promote dignity when interactions are characterized by symmetrical social encounters. Dignity in the individual arena centers on foodways, cultural or familial traditions, and role-taking as a food provider. In the institutional arena, dignity is influenced by structures and operational approaches. Our research contributes to literatures informing policies and strategies employed by community-led, rights-based food aid systems in advanced capitalist nations. Efforts prioritize and promote human dignity, despite neoliberal, advanced capitalist governments’ failure to address structural inequalities as a root cause of food insecurity.
Dynamic Control of Integrated Wind Farm Battery Energy Storage Systems for Grid Connection
Authors: Gwabavu Mandisi , Raji Atanda
Published: March 2021

The intermittent nature of wind power is a major challenge for wind as an energy source. Wind power generation is therefore difficult to plan, manage, sustain, and track during the year due to different weather conditions. The uncertainty of energy loads and power generation from wind energy sources heavily affects the system stability. The battery energy storage system (BESS) plays a fundamental role in controlling and improving the efficiency of renewable energy sources. Stochasticity of wind speed and reliability of the main system components are considered. This paper presents a dynamical control system based on model predictive control (MPC) in real time, to make full use of the flexibility and controllability of energy storage to mitigate problems of wind farm variability and intermittency. The control scheme first plans the expected output, then stochastic optimization is used to optimize grid integrated wind farm BESS output power, develop an optimal operation strategy for BESS, and prevent some unpredictable conditions that may have impacts on the stability of the system. The results show that the proposed method can reduce grid-connected wind power fluctuations, limit system faults, control command for the BESS in the dispatching period, and ensure system stability for grid connection.
Analysis of BIM Methodology Applied to Practical Cases in the Preservation of Heritage Buildings
Authors: Sampaio Alcinia Zita , Gomes Augusto Martins , Sánchez-Lite Alberto , Zulueta Patricia , González-Gaya Cristina
Published: March 2021

The methodology and technology associated with building information modeling (BIM) provide architects, engineers, and historians with concepts and tools that support the development of heritage projects. However, this specific form of BIM orientated towards buildings of patrimonial value—known as historic building information modeling (HBIM)—requires a distinct and additional view, accounting for aspects which are normally not attended to on projects involving new buildings. In an HBIM context, the parametric modeling process, the basis of any BIM procedure, involves the study of shapes, patterns, or standards for the establishment of particular collections of parametric objects, as well as the record of the available technology used to capture digital geometric data. In addition, all the information collected and generated through an HBIM process must be adequately managed, maintained, and archived. In the present study, we intend to list the most recent features of HBIM, based on a bibliographic review, encompassing distinct building situations (preservation, restoration, rehabilitation, and structural assessment); different technical equipment (drones, scanners, and photogrammetry); as well as diverse forms of geometric characterization (patterns, geometric rules, or curve generation) and ways of archiving data (stratigraphy, old drawings folders, or as-built models). With the aim of identifying, as an overview, we have presented the principal modeling strategies, technologic devices, and archive procedures, as a contribution to systematizing and organizing the dispersed practical and theorical studies related with HBIM.
The Vega Alta of Segura River (Southeast of Spain): A Wetland of International Importance
Authors: Ballesteros-Pelegrín Gustavo , Ibarra-Marinas Daniel , García-Marín Ramón
Published: March 2021

The Ramsar Convention is an intergovernmental treaty for the conservation and wise use of wetlands, which establishes nine criteria related to natural values and a cultural one that wetlands must meet to be included in the list of wetlands of international importance. We aim to evaluate if the wetlands of the Vega Alta of the Segura River (southeast of Spain) meet the requirements to fulfil this agreement. Thanks to meticulous fieldwork and a bibliographic review related to the stated objective, we collected information on the existing environmental and cultural values. The results show that this set of wetlands is home to 11 species of threatened vertebrates in Spain, two priority habitats in the European Union, as well as cultural values related to their origin, conservation, and ecological functioning. Likewise, in the area there are archaeological sites, traditional uses of water associated with the cultivation of rice, and religious manifestations. Effective wetland inventories and rigorous analyzes of their ecological and environmental characteristics, as well as their socioeconomic functions, need to be carried out in order to improve their management and protection. This wetland certainly meets three criteria to be included in the Ramsar Convention list of wetlands.
Envisioning a Circular Economy: The Journey of One Mid-Sized Midwestern City
Authors: Petoskey Jennifer , Stults Missy , Naples Eileen , Hardy Galen , Quilici Alicia , Byerly Cassie , Clark Amelia , Newton Deja , Santiago Elizabeth , Teener Jack
Published: March 2021

The City of Ann Arbor has committed to a just and equitable transition to community-wide carbon neutrality by 2030. Our guiding plan, A2ZERO, outlines seven strategies and 44 actions that were chosen by the community to achieve this goal. One of the seven strategies is “Changing the Way We Use, Reuse, and Dispose of Materials”, including the action: “move toward a circular economy”. Many cities are trying to move towards a circular economy, tailoring policies, actions, and outreach towards their unique circumstances. Regardless of context, becoming circular requires an array of actions including collaboration and partnership, policy setting, program development, and education. This paper explores how the concept of the circular economy is discussed in the peer reviewed literature and in practitioner circles, exploring similarities and differences. Following this, we undertake a critical instance case study on the City of Ann Arbor’s materials management programs and efforts towards achieving a more circular local economy. We conclude by offering pathways that Ann Arbor and other cities across the U.S. can pursue to advance a circular economy.
Visualizing Digital Traces for Sustainable Urban Management: Mapping Tourism Activity on the Virtual Public Space
Authors: Valls Francesc , Roca Josep
Published: March 2021

One of the challenges of heritage cities is sustainably balancing mass tourism and the daily life of its residents. Urban policies can modulate the impact of tourism through regulations focusing on areas with outstanding visitor pressure, which must consequently be delimited accurately and objectively. Within a traditionally data-scarce discipline, urban practitioners can currently employ a wide range of tracking technologies, but because of their limitations can also greatly benefit from new sources of data from social media. Using Barcelona as a testbed, a methodology is presented to identify and visualize hot spots of visitor activity using more than a million public geotagged images collected from the Flickr photo-sharing community. Multiple complementary visualization approaches are discussed that are suitable for different scales of analysis, from global to sub-block resolution. The presented methodology is firmly grounded in a well-established spatial statistics framework, adapted to a “big data” environment, to extract knowledge from social media. It is designed to generalize to other urban settings, providing substantial advantages over other surveying methods in terms of cost-efficiency, scalability, and accuracy, while capturing the behavior of a larger number of participants and covering more extensive areas or temporal spans.
Impacts of Employee Empowerment and Organizational Commitment on Workforce Sustainability
Authors: Murray William C. , Holmes Mark R.
Published: March 2021

Building and maintaining a sustainable workforce in the hospitality sector, where demand for talent consistently exceeds supply across the globe, has only been exacerbated by COVID-19. The need to sustain this workforce behooves the industry to unpack core drivers of employee commitment in order to retain top talent. This paper explores how dimensions of employee empowerment increase organizational commitment and, in turn, reduce turnover intention—leading to a more sustained workforce. Drawing on the results of 346 surveys within the Canadian lodging industry, structural equation modeling was undertaken to examine the influence of empowerment on organizational commitment and organizational commitments influence on turnover intention. Findings suggest that the development of meaning through employee empowerment, particularly when the ideals and standards between workers and their organization are aligned, creates a strong emotional commitment which appears to strongly reduce an employee’s intention to leave. Feelings of emotional connection or duty towards an organization show clear positive relationships with reduced intentions to leave. For an industry struggling with higher-than-average turnover intention and labour costs, focusing on creating work with meaning, and instilling a sense of belonging in the workforce will enable organizations to reduce their employee’s turnover intentions.
Research on the Optimization of Empty Container Repositioning of China Railway Express in Cooperation with International Liner Companies
Authors: Xing Lei , Yan Hong , Yin Yandong , Xu Qi
Published: March 2021

Both China Railway Express (CRE) companies and international liner companies are faced with the problem of empty container repositioning. In order to reduce empty container repositioning cost and realize their sustainable development, this paper studies the optimization problem of empty container repositioning under the condition of their cooperation. To minimize the cost, three optimization models of empty container repositioning are established, which are based on the single repositioning and cooperative repositioning. Numerical experiments are carried out to analyze the three empty container repositioning optimization models solved by CPLEX. The results show that the total cost could be greatly reduced by the cooperative repositioning. The effects of cooperation become more obvious with the unit storage cost or repositioning cost increases and become weaker with the unit mutual rental cost increase. When the demand fluctuation is in a certain range, the cooperation is still effective, which can reduce the cost. But when it is beyond a certain range, the benefits will be greatly reduced. In reality, for the sustainability of their cooperation, both sides should pay attention to the proportion of supply and demand and set reasonable mutual rental cost.
Public–Private Partnerships for Climate Technology Transfer and Innovation: Lessons from the Climate Technology Centre and Network
Authors: Lee Woo Jin , Juskenaite Irma , Mwebaza Rose
Published: March 2021

The 2030 Agenda for Sustainable Development and the Sustainable Development Goals (SDGs), the Paris Agreement, and a number of important agreements call on the United Nations (UN) to strengthen a relationship with the private sector to develop and transfer climate technology in global action on climate change. The Technology Mechanism (TM) is anchored in the UN Framework Convention on Climate Change as a key enabler for the attainment of the Goals of the Paris Agreement. The growing interest for collaboration with the private sector sets new ambitions for the UN Climate Technology Centre and Network (CTCN). The active engagement of the private sector is critical for successful technology transfer and successful innovation. This paper reviews and analyses the role of the private sector in facilitating technology transfer in CTCN’s Technical Assistance. Furthermore, the shared interest for partnership between the CTCN and the private sector was evaluated by analysing in-depths interviews with major CTCN stakeholders. Based upon this analysis, several recommendations are made on how to enhance public–private partnerships in order to strengthen private sector participation in climate technology transfer activities with a special focus on technology–push and market–pull innovation.
Emergency Surgery in the Elderly: Could Laparoscopy Be Useful in Frailty? A Single-Center Prospective 2-Year Follow-Up in 120 Consecutive Patients
Authors: Zese Monica , Finotti Elena , Cestaro Giovanni , Cavallo Fabio , Prando Daniela , Gobbi Tobia , Zese Riccardo , Di Saverio Salomone , Agresta Ferdinando
Published: March 2021

Background: the general population is aging across the world. Therefore, even surgical interventions in the elderly—in particular those involving emergency surgical admissions—are becoming more frequent. The elderly population is often frail (in multiple physiological systems, this is often defined as age-related cumulative decline). This study involved a 2-year follow-up evaluation of frail elderly patients treated with urgent surgical intervention at Santa Maria Regina della Misericordia Hospital, General Surgery Department, in Adria (Italy). Method: a prospective, single-center, 2-year follow-up study of 120 patients >65 years old, treated at our department for surgical abdominal emergencies. We considered co-morbidities (ASA—American Society of Anesthesiologists Physical Status Classification System—score), type of surgery (laparoscopy, laparotomy or converted), frailty score, mortality, and complications at 30 days and at 2 years. Conclusions: 70 (58.4%) patients had laparoscopy, 49 (40.8) had laparotomy, and in 1 (0.8%) case, surgery was converted from laparoscopy to laparotomy. Mortality strictly depends on the type of surgery (laparotomy vs. laparoscopy), complications during recovery, and a lower Fried frailty criteria score, on average. The long-term follow-up can be a useful tool to highlight a safer surgical approach, such as laparoscopy, in frail elderly patients. We consider the laparoscopic approach feasible in emergency situations, with similar or better outcomes than laparotomy, especially in frail elderly patients.
The Flexible Burr X-G Family: Properties, Inference, and Applications in Engineering Science
Authors: Al-Babtain Abdulhakim A. , Elbatal Ibrahim , Al-Mofleh Hazem , Gemeay Ahmed M. , Afify Ahmed Z. , Sarg Abdullah M.
Published: March 2021

In this paper, we introduce a new flexible generator of continuous distributions called the transmuted Burr X-G (TBX-G) family to extend and increase the flexibility of the Burr X generator. The general statistical properties of the TBX-G family are calculated. One special sub-model, TBX-exponential distribution, is studied in detail. We discuss eight estimation approaches to estimating the TBX-exponential parameters, and numerical simulations are conducted to compare the suggested approaches based on partial and overall ranks. Based on our study, the Anderson–Darling estimators are recommended to estimate the TBX-exponential parameters. Using two skewed real data sets from the engineering sciences, we illustrate the importance and flexibility of the TBX-exponential model compared with other existing competing distributions.
The Use of Sodium Hypochlorite at Point-of-Use to Remove Microcystins from Water Containers
Authors: Mokoena Matodzi Michael , Mudau Lutendo Sylvia , Mokgobu Matlou Ingrid , Mukhola Murembiwa Stanley
Published: March 2021

Most conventional water treatment plants are not sufficiently equipped to treat both intracellular and extracellular Microcystins in drinking water. However, the effectiveness of sodium hypochlorite in removing Microcystin in containers at the point-of-use is not yet known. This study aimed to assess point-of-use water container treatment using bleach or sodium hypochlorite (NaOCl) and to assess the health problems associated with microcystins. Thirty-nine percent (29 of 74) of the total selected households were randomly selected to receive and treat their stored container water with sodium hypochlorite. The level of microcystin in the container water was measured after 30 min of contact with sodium hypochlorite. Microcystin concentrations in both the blooming and decaying seasons were higher (mean 1.10, 95% CI 0.46–1.67 µg/L and mean 1.14, 95% CI 0.65–1.63 µg/L, respectively) than the acceptable limit of 1 µg/L in households that did not treat their water with NaOCl, whilst in those that did, there was a significant reduction in the microcystin concentration (mean 0.07, 95% CI 0.00–0.16 µg/L and mean 0.18, 95% CI 0.00–0.45 µg/L). In conclusion, sodium hypochlorite treatment decreased microcystin s to an acceptable level and reduced the related health problems.
Comparison of Preclinical Properties of Several Available Antivenoms in the Search for Effective Treatment of Vipera ammodytes and Vipera berus Envenoming
Authors: Kurtović Tihana , Lang Balija Maja , Brvar Miran , Dobaja Borak Mojca , Mateljak Lukačević Sanja , Halassy Beata
Published: March 2021

Snakebites are a relatively rare medical emergency in Europe. In more than half of the annual cases caused by Vipera ammodytes, Vipera berus, and Vipera aspis, immunotherapy with animal-derived antivenom is indicated. Among eight products recently identified as available against European medically relevant species, only Zagreb antivenom, Viperfav, and ViperaTAb have been used almost exclusively for decades. Zagreb antivenom comprises V. ammodytes-specific F(ab′)2 fragments. Viperfav is a polyspecific preparation based on F(ab′)2 fragments against V. aspis, V. berus, and V. ammodytes venoms. ViperaTAb contains Fab fragments against the venom of V. berus. In 2014 the production of Zagreb antivenom was discontinued. Additionally, in the period of 2017 to 2018 a shortage of Viperfav occurred. Due to a lack of the product indicated for the treatment of V. ammodytes bites, other antivenoms were implemented into clinical practice without comparative assessment of their eligibility. The aim of our work was to identify a high-quality antivenom that might ensure the successful treatment of V. ammodytes and V. berus bites at the preclinical level. Differentiation between bites from these two species is difficult and unreliable in clinical practice, so the availability of a unique antivenom applicable in the treatment of envenoming caused by both species would be the most advantageous for Southeastern Europe. Zagreb antivenom, Viperfav, and ViperaTAb, as well as Viper venom antitoxin for V. berus envenoming and the in-development Inoserp Europe, which was designed to treat envenoming caused by all medically important European snakes, were comparatively tested for the first time. Emphasis was placed on their physicochemical properties, primarily purity and aggregate content, as well as their in vivo protective efficacies. As Zagreb antivenom is no longer available on the European market, Viperfav is the highest-quality product currently available and the only antivenom whose neutralisation potency against V. ammodytes and V. berus venoms was above regulatory requirements.
Issues and Controversies in the Evolution of Oral Rehydration Therapy (ORT)
Authors: Nalin David
Published: March 2021

The original studies demonstrating the efficacy of oral glucose-electrolytes solutions in reducing or eliminating the need for intravenous therapy to correct dehydration caused by acute watery diarrheas (AWD) were focused chiefly on cholera patients. Later research adapted the oral therapy (ORT) methodology for treatment of non-cholera AWDs including for pediatric patients. These adaptations included the 2:1 regimen using 2 parts of the original WHO oral rehydration solution (ORS) formulation followed by 1 part additional plain water, and a “low sodium” packet formulation with similar average electrolyte and glucose concentrations when dissolved in the recommended volume of water. The programmatic desire for a single ORS packet formulation has led to controversy over use of the “low sodium” formulations to treat cholera patients. This is the subject of the current review, with the conclusion that use of the low-sodium ORS to treat cholera patients leads to negative sodium balance, leading to hyponatremia and, in severe cases, particularly in pediatric cholera, to seizures and other complications of sodium depletion. Therefore it is recommended that two separate ORS packet formulations be used, one for cholera therapy and the other for non-cholera pediatric AWD.
Physical Acceptability of the Renyi, Tsallis and Sharma-Mittal Holographic Dark Energy Models in the f(T,B) Gravity under Hubble’s Cutoff
Authors: Shekh Salim Harun , Moraes Pedro H. R. S. , Sahoo Pradyumn Kumar
Published: March 2021

In the present article, we investigate the physical acceptability of the spatially homogeneous and isotropic Friedmann–Lemâitre–Robertson–Walker line element filled with two fluids, with the first being pressureless matter and the second being different types of holographic dark energy. This geometric and material content is considered within the gravitational field equations of the f(T,B) (where T is the torsion scalar and the B is the boundary term) gravity in Hubble’s cut-off. The cosmological parameters, such as the Equation of State (EoS) parameter, during the cosmic evolution, are calculated. The models are stable throughout the universe expansion. The region in which the model is presented is dependent on the real parameter δ of holographic dark energies. For all δ≥4.5, the models vary from ΛCDM era to the quintessence era.
Asymptotically Flat Boundary Conditions for the U(1)3 Model for Euclidean Quantum Gravity
Authors: Bakhoda Sepideh , Shojaie Hossein , Thiemann Thomas
Published: March 2021

A generally covariant U(1)3 gauge theory describing the GN→0 limit of Euclidean general relativity is an interesting test laboratory for general relativity, specially because the algebra of the Hamiltonian and diffeomorphism constraints of this limit is isomorphic to the algebra of the corresponding constraints in general relativity. In the present work, we the study boundary conditions and asymptotic symmetries of the U(1)3 model and show that while asymptotic spacetime translations admit well-defined generators, boosts and rotations do not. Comparing with Euclidean general relativity, one finds that the non-Abelian part of the SU(2) Gauss constraint, which is absent in the U(1)3 model, plays a crucial role in obtaining boost and rotation generators.
The Mechanism of PEDV-Carrying CD3+ T Cells Migrate into the Intestinal Mucosa of Neonatal Piglets
Authors: Yuan Chen , Li Yuchen , Zhang En , Jin Yuxin , Yang Qian
Published: March 2021

Porcine epidemic diarrhea virus (PEDV) can cause intestinal infection in neonatal piglets through the nasal cavity. A process in which CD3+ T cells carry PEDV plays a key role. However, the modes through which PEDV bridles CD3+ T cells as a vehicle for migration to the intestinal epithelium have not been clarified. In this study, we first demonstrated that PEDV could survive in blood-derived CD3+ T cells for several hours, depending on the multiplicity of infection. In addition, PEDV preferentially survived in CD4+ T cells over CD8+ T cells. Moreover, viral transmission was mediated by cell-to-cell contact between mesenteric lymph-node-derived CD3+ T cells, but did not occur in blood-derived CD3+ T cells. Following an increase in gut-homing integrin α4β7, blood-derived CD3+ T cells carrying PEDV migrated to the intestines via blood circulation and transferred the virus to intestinal epithelial cells through cell-to-cell contact in neonatal piglets. Our findings have significant implications for understanding PEDV pathogenesis in neonatal piglets, which is essential for developing innovative therapies to prevent PEDV infection.
Analysis of Different Types of Interferon-Associated Retinopathy in Patients with Chronic Hepatitis C Virus Infection Treated with Pegylated Interferon Plus Ribavirin
Authors: Wu Chia-Min , Su Fu-Hsiung , Muo Chih-Hsin , Huang Jou-Chen , Wu Meei-Maan , Yeh Chih-Ching
Published: March 2021

This retrospective cohort study aims to investigate interferon (IFN)-associated retinopathy incidence in patients with chronic hepatitis C virus (HCV) infection treated with pegylated interferon (PegIFN) plus ribavirin (RBV). We selected 1688 patients undergoing PegIFN/RBV therapy for HCV (HCV-treated cohort), 3376 patients not receiving HCV treatment (HCV-untreated cohort) and 16,880 controls without HCV (non-HCV cohort) from the Taiwan Longitudinal Health Insurance Database. The patients were frequency-matched by age, sex, and index date at a 1:2:10 ratio, and followed up until the end of 2013. Cox proportional hazard regression models were used to compare the incidences of any retinal vascular events, including subtypes, among the three cohorts. Compared with the non-HCV cohort, the HCV-treated cohort had a significantly increased risk of retinopathy (hazard ratio (HR) = 4.98, 95% confidence interval (CI): 2.02–12.3). The risk was particularly prominent for retinal hemorrhage (HR = 12.7, 95% CI: 3.78–42.9). When the HCV-untreated cohort was used as the reference, the aforementioned HRs increased to 9.02 (95% CI: 3.04–26.8) and 32.3 (95% CI: 3.94–265), respectively. This study suggested that PegIFN/RBV therapy significantly increased the risk of retinal hemorrhage but not retinal vascular occlusions in the HCV-treated cohort.
Development of a Potent Stabilizer for Long-Term Storage of Foot-and-Mouth Disease Vaccine Antigens
Authors: Kim Ah-Young , Kim Hyejin , Park Sun Young , Park Sang Hyun , Kim Jae-Seok , Park Jung-Won , Park Jong-Hyeon , Ko Young-Joon
Published: March 2021

A local virus isolate, O/SKR/JC/2014 (O JC), has been considered as a candidate vaccine strain in the development of a domestic foot-and-mouth disease (FMD) vaccine in Korea. However, producing and preserving a sufficient quantity of intact vaccine antigens from the O JC strain was difficult owing to its distinctive structural instability compared to other candidate vaccine strains. Based on this feature, the O JC strain was adopted as a model virus for the stabilization study to determine the optimal stabilizer composition, which enables long-term storage of the FMD vaccine antigen in both aqueous and frozen phases. In contrast to O JC vaccine antigens stored in routinely used Tris-buffered or phosphate-buffered saline, those stored in Tris-KCl buffer showed extended shelf-life at both 4 °C and −70 °C. Additionally, the combined application of 10% sucrose and 5% lactalbumin hydrolysate could protect O JC 146S particles from massive structural breakdown in an aqueous state for up to one year. The stabilizer composition was also effective for other FMDV strains, including serotypes A and Asia 1. With this stabilizer composition, FMD vaccine antigens could be flexibly preserved during the general production process, pending status under refrigeration and banking under ultrafreezing.
A Fermented Milk Matrix Containing Postbiotics Supports Th1- and Th17-Type Immunity In Vitro and Modulates the Influenza-Specific Vaccination Response In Vivo in Association with Altered Serum Galectin Ratios
Authors: Ayechu-Muruzabal Veronica , Xiao Ling , Wehkamp Tjalling , van Ark Ingrid , Hoogendoorn Elisabeth J. , Leusink-Muis Thea , Folkerts Gert , Garssen Johan , Willemsen Linette E. M. , van’t Land Belinda
Published: March 2021

During a specific milk fermentation process with Bifidobacterium breve C50 and Streptococcus thermophilus 065 (LactofidusTM), postbiotics with possible immunomodulatory properties are produced. We investigated the effects of this fermentation product (FP) in vitro using a model that allows crosstalk between intestinal epithelial (IEC) and immune cells. IECs were exposed to FP and αCD3/CD28-activated peripheral blood mononuclear cells after which the mediator secretion was measured. Additionally, using a murine influenza vaccination model, immune development was assessed. Mice were fed an AIN93G diet containing FP or lactose as control. Vaccine-specific immunity was measured as delayed-type hypersensitivity (DTH) and correlated to intestinal and systemic immunomodulation levels. In vitro, exposure to FP enhanced IFNγ, TNFα and IL-17A concentrations. Moreover, IEC-derived galectin-3/galectin-9 and galectin-4/galectin-9 ratios were increased. In vivo, dietary intervention with FP increased vaccine-specific DTH responses as compared to the lactose-receiving group. Although no effects on humoral immunity and vaccine-specific T-cell responses were detected, an enhanced systemic serum galectin-3/galectin-9 and galectin-4/galectin-9 ratio correlated with a shift in RORγ (Th17) mRNA expression over regulatory TGFβ1 in the ileum. This was also positively correlated with the increased DTH response. These results indicate that FP can enhance epithelial galectin-3 and -4 over galectin-9 release, and boost adaptive immunity by promoting Th1- and Th17-type cytokines under inflammatory conditions in vitro. Similar variations in galectin and immune balance were observed in the vaccination model, where FP improved the influenza-specific DTH response.
Vaccination Coverage for Routine Vaccines and Herd Immunity Levels against Measles and Pertussis in the World in 2019
Authors: Plans-Rubió Pedro
Published: March 2021

In 2012, the World Health Organization (WHO) established the Global Vaccine Action Plan with the objective to promote essential vaccinations in all countries and achieve at least 90% vaccination coverage for all routine vaccines by 2020. The study assessed the mean percentages of vaccination coverage in 2019 for 13 routine vaccines, vaccination coverage variation from 2015 to 2019, and herd immunity levels against measles and pertussis in 2019 in countries and regions of WHO. In 2019, the mean percentages of vaccination coverage were lower than 90% for 10 (78.9%) routine vaccines. The mean percentages of vaccination coverage also decreased from 2015 to 2019 for six (46.2%) routine vaccines. The prevalence of individuals with vaccine-induced measles immunity in the target measles vaccination population was 88.1%, and the prevalence of individuals with vaccine-induced pertussis immunity in the target pertussis vaccination population was 81.1%. Herd immunity against measles viruses with Ro = 18 was established in 63 (32.5%) countries but not established in any region. Herd immunity against pertussis agents was not established in any country and in any region of WHO. National immunization programs must be improved to achieve ≥90% vaccination coverage in all countries and regions. Likewise, it is necessary to achieve ≥95% vaccination coverage with two doses of measles vaccines and three doses of pertussis vaccines in all countries and regions.
Potential Use of the Benthic Foraminifers Bulimina denudata and Eggerelloides advenus in Marine Sediment Toxicity Testing
Authors: McGann Mary
Published: March 2021

The benthic foraminifers Bulimina denudata and Eggerelloides advenus are commonly abundant in offshore regions in the Pacific Ocean, especially in waste-discharge sites. The relationship between their abundance and standard macrofaunal sediment toxicity tests (amphipod survival and sea urchin fertilization) as well as sediment chemistry analyte measurements were determined for sediments collected in 1997 in Santa Monica Bay, California, USA, an area impacted by historical sewage input from the Hyperion Outfall primarily since the late 1950s. Very few surface samples proved to be contaminated based on either toxicity or chemistry tests and the abundance of B. denudata did not correlate with any of these. The abundance of E. advenus also did not correlate with toxicity, but positively correlated with total solids and negatively correlated with arsenic, beryllium, chromium, lead, mercury, nickel, zinc, iron, and TOC. In contrast, several downcore samples proved to be contaminated as indicated by both toxicity and chemistry data. The abundance of B. denudata positively correlated with amphipod survival and negatively correlated with arsenic, cadmium, unionized ammonia, and TOC; E. advenus negatively correlated with sea urchin fertilization success as well as beryllium, cadmium, and total PCBs. As B. denudata and E. advenus are tolerant of polluted sediments and their relative abundances appear to track those of macrofaunal toxicity tests, their use as cost- and time-effective marine sediment toxicity tests may have validity and should be further investigated.
Medieval Relict Beaver Ponds in the Polish Plain: Studies from the Tuchola Forest
Authors: Śnieszko Zbigniew , Rurek Mirosław , Hojan Marcin
Published: March 2021

Beavers have lived in the territory of Poland since the beginning of the Holocene, as testified by bone remains found in archaeological sites of different ages. The area inhabited by these animals has experienced continuing transformations of terrain relief, geological structure, hydrology and plant cover. In Poland, beavers are partially protected and their population has spread in virtually every part of the country (except in the highest mountain ranges). The authors of this paper wish to present the results of field works carried out since 2006 in the Tuchola Forest (Polish Plain). This paper aims to identify the potential sediments of relict beaver ponds and their sedimentological features. The studies are also backed up with a description of radiocarbon dating of samples. The results indicate that beavers used to live in the Tuchola Forest in the Middle Ages, as shown by the radiocarbon dates and sequences of mineral–organic deposits found in exposures and geological boreholes. The spatial distribution of organic and mineral deposits in wider sections of river valleys can be explained by the avulsion of the riverbed downstream of the pond and by the distribution of ponds in the Gołyjonka valley. The discovery of relict beaver pond sediments suggests that the activity of these mammals in the Middle Ages played a major part in shaping the landscape of the valley. The results of studies clearly indicate that analyses of the valley sediment facies of small watercourses should take into account the role beavers played in the past in shaping the landscape of the analysed valley. This highlights the insufficiency of studies concerning the activity of beavers in river valleys.
Influences of Dimethyl Phthalate on Bacterial Community and Enzyme Activity in Vertical Flow Constructed Wetland
Authors: Xu QiaoLing , Lu ShaoYong , Yuan Tao , Zhang Feng , Wang Li , Wang Ping , Wen XueYuan , Cui LiHua
Published: March 2021

Dimethyl phthalate (DMP), belonging to the family of Phthalate esters (PAEs), is a plasticizer and has been widely used in the world for many years. Nowadays, it has become a ubiquitous environmental pollutant and is listed as an environmental priority pollutant by China’s Environmental Monitoring Center. The purpose of this study is to estimate the responses of the bacterial community and enzyme activity to DMP contamination in three vertical flow constructed wetlands (VFCW), namely the constructed wetland A (planted with Pennisetum sinese Roxb), constructed wetland B (planted with Pennisetum purpureum Schum.), and constructed wetland C (unplanted), respectively. The results showed that the relative percentages of some genera associated with nitrogen metabolism and the function of degrading aromatic hydrocarbons were increased by DMP contamination, such as Dechloromonas agitata, Pleomorphomonas sp., Denitratisoma oestradiolicum, Plasticicumulans lactativorans, Novosphingobium sp., Alicycliphilus denitrificans, and Thauera sp. Meanwhile, principal coordinate analysis (PCA) analysis showed that the addition of DMP divided 12 samples into two groups as followed: one was the DMP group containing a-1, a-2, b-1, b-2, c-1 and c-2 while the other was no DMP group including A-1, A-2, B-1, B-2, C-1 and C-2. It indicated that DMP was the main reason for this change. In addition, by monitoring the activity of substrate enzymes, the activity of urease, phosphatase, catalase, and invertase in the wetlands before and after the experiment, these were significantly higher in the upper layer than in the lower layer and maintained high activity. Ultimately, the average influent concentration of DMP in three VFCWs was 8.12 mg/L and the average removal efficiency of the effluent was over 90%. Our results suggested that DMP was an important factor affecting the microbial community structure of wetland and the upper layer of the VFCW was the main site for the degradation of DMP. VFCW has great potential for the removal of the high concentration of DMP and it can be a good choice for the treatment of PAEs.
Research on Descaling Characteristics and Simulation Calculation of a Coaxial High-Frequency Electronic Descaling Device
Authors: Zhao Yan , Zhang Lixin , Zhao Xin , Liu Jin , Gao Ming
Published: March 2021

High-frequency electronic descaling devices are physical water treatment methods that use a high-frequency electromagnetic field to prevent and remove scale. The effectiveness of the method is verified by monitoring the growth of scale on the surface of heat exchange tubes. The microstructure of scale obtained from experiments is analyzed by scanning electron microscope (SEM), and the action characteristics of high-frequency electromagnetic fields on water are explored by observing the change of solution contact angle at different times. The experimental results show that the high-frequency electromagnetic field can slow down the scaling growth on the surface of heat exchange tubes by changing the morphology of scaling substances and the physicochemical properties of water. The cavity of the instrument is modeled and simulated by ANSYS Maxwell, and the three operating parameters, waveform, voltage and frequency, are changed respectively. The performance parameters of the cavity, such as magnetic field energy, electric field energy and magnetic flux, are calculated and compared, and then the more suitable operating parameters are selected to improve the performance of the instrument. The simulation results show that the high-frequency electromagnetic field generated by the anode rod in the axial position can be overlooked compared with the magnetic field energy. Square wave excitation produces greater magnetic field energy than using sine wave excitation, and as the voltage increases, the peak value of the magnetic field energy continues to rise and increases faster. With an increase in the frequency, the peak value of the magnetic field energy and magnetic flux peak will maintain a slight decrease over a certain frequency range. After this frequency range, the peak value of magnetic field energy and magnetic flux peak will decrease rapidly. This decrease is due to the relaxation caused by the change of the waveform direction. The influence of time and an increase in the frequency will significantly increase the influence of the relaxation time.
Stability Control for Electric Vehicles with Four In-Wheel-Motors Based on Sideslip Angle
Authors: Yang Kun , Dong Danxiu , Ma Chao , Tian Zhaoxian , Chang Yile , Wang Ge
Published: March 2021

Tire longitudinal forces of electrics vehicle with four in-wheel-motors can be adjusted independently. This provides advantages for its stability control. In this paper, an electric vehicle with four in-wheel-motors is taken as the research object. Considering key factors such as vehicle velocity and road adhesion coefficient, the criterion of vehicle stability is studied, based on phase plane of sideslip angle and sideslip-angle rate. To solve the problem that the sideslip angle of vehicles is difficult to measure, an algorithm for estimating the sideslip angle based on extended Kalman filter is designed. The control method for vehicle yaw moment based on sliding-mode control and the distribution method for wheel driving/braking torque are proposed. The distribution method takes the minimum sum of the square for wheel load rate as the optimization objective. Based on Matlab/Simulink and Carsim, a cosimulation model for the stability control of electric vehicles with four in-wheel-motors is built. The accuracy of the proposed stability criterion, the algorithm for estimating the sideslip angle and the wheel torque control method are verified. The relevant research can provide some reference for the development of the stability control for electric vehicles with four in-wheel-motors.