bluuebunny/crossref_metadata_2025 huggingface dataset:
Job Demands, Resources and Strains of Outpatient Caregivers during the COVID-19 Pandemic in Germany: A Qualitative Study
Authors: Mojtahedzadeh Natascha , Wirth Tanja , Nienhaus Albert , Harth Volker , Mache Stefanie
Published: April 2021

The COVID-19 pandemic has affected health professionals in a special way, as they are responsible for the care of vulnerable groups. Little is known about how outpatient caregivers perceive their working conditions during the pandemic in Germany and about the difficulties they face. The aims of this study were (1) to examine specific job demands of outpatient caregivers in regard to the COVID-19 pandemic, (2) to illuminate their job resources they can rely on and (3) to identify potential strain reactions they experience. Fifteen semi-structured telephone interviews were conducted with outpatient caregivers working in Northern Germany in the period May–June 2020. Interviews were analyzed by using qualitative content analysis. Outpatient caregivers experienced daily mask obligation, lack of personal protection equipment (PPE) and stricter hygiene regulations as demanding during the pandemic. They also described a higher workload and emotional demands such as fear of infection or infecting others. They perceived team spirit and communication as important work-related resources. Depressive symptoms and feelings of stress were described as strain reactions. Outpatient care services need to be better prepared for sudden pandemic situations and provide their employees with sufficient PPE and education to reduce pandemic-related job demands leading to negative strain reactions.
Consumer Food Environment Healthiness Score: Development, Validation, and Testing between Different Types of Food Retailers
Authors: Borges Camila Aparecida , Gabe Kamila Tiemann , Jaime Patricia Constante
Published: April 2021

The aim of this study was to develop and validate a scoring system, based on AUDITNOVA, to assess the healthiness of the consumer food environment, considering food availability, price, advertising, and placement strategies. Audited data of 650 food retailers were used to develop, validate, and test the consumer food environment healthiness score. To compose the score, the reference was the Dietary Guidelines for the Brazilian Population. The total and subscores were standardized for a scale from 0 to 100. Construct validity was assessed using the Kruskal–Wallis Dunn tests. Cronbach’s alpha coefficients were calculated to determine the consistency of the scores. The median score was 33.7 (p25 = 26.9; p75 = 42.1). The public and private specialized indoor fresh food markets showed the highest medians; otherwise, bakeries and food retailers with the predominant sale of ultra-processed foods showed the lowest. The score was able to satisfactorily classify the extreme food retailer groups by the predominant sale of fresh or minimally processed foods and the predominant sale of ultra-processed foods. The results of Cronbach’s alpha showed excellent internal consistency (α = 0.91). The score helped to provide an overall assessment of consumer food environment healthiness and was able to classify food retailer groups as healthy and unhealthy according to the degree of processing of the available foods.
Predicting the Unknown and the Unknowable. Are Anthropometric Measures and Fitness Profile Associated with the Outcome of a Simulated CrossFit® Competition?
Authors: Peña Javier , Moreno-Doutres Daniel , Peña Iván , Chulvi-Medrano Iván , Ortegón Alberto , Aguilera-Castells Joan , Buscà Bernat
Published: April 2021

The main objective of this research was to find associations between the outcome of a simulated CrossFit® competition, anthropometric measures, and standardized fitness tests. Ten experienced male CrossFit® athletes (age 28.8 ± 3.5 years; height 175 ± 10.0 cm; weight 80.3 ± 12.5 kg) participated in a simulated CrossFit® competition with three benchmark workouts (“Fran”, “Isabel”, and “Kelly”) and underwent fitness tests. Participants were tested for anthropometric measures, sit and reach, squat jump (SJ), countermovement jump (CMJ), and Reactive Strength Index (RSI), and the load (LOAD) corresponding to the highest mean power value (POWER) in the snatch, bench press, and back squat exercises was determined using incremental tests. A bivariate correlation test and k-means cluster analysis to group individuals as either high-performance (HI) or low performance (LO) via Principal Component Analysis (PCA) were carried out. Pearson’s correlation coefficient two-tailed test showed that the only variable correlated with the final score was the snatch LOAD (p < 0.05). Six performance variables (SJ, CMJ, RSI, snatch LOAD, bench press LOAD, and back squat LOAD) explained 74.72% of the variance in a k = 2 means cluster model. When CrossFit® performance groups HI and LO were compared to each other, t-test revealed no difference at a p ≤ 0.05 level. Snatch maximum power LOAD and the combination of six physical fitness tests partially explained the outcome of a simulated CrossFit competition. Coaches and practitioners can use these findings to achieve a better fit of the practices and workouts designed for their athletes.
Migrant Agricultural Workers’ Health, Safety and Access to Protections: A Descriptive Survey Identifying Structural Gaps and Vulnerabilities in the Interior of British Columbia, Canada
Authors: Colindres Carlos , Cohen Amy , Caxaj C. Susana
Published: April 2021

In this paper, we provide descriptive data that characterize the health, safety, and social care environment of migrant agricultural workers in British Columbia, Canada. Through the administration of surveys (n = 179), we gathered information in three domains: (1) living and working conditions; (2) barriers to rights, health, safety and advocacy/reporting; (3) accessibility of services. Our study confirms what predominantly qualitative studies and Ontario-based survey data indicate in terms of health, legal, and social barriers to care and protection for this population. Our findings also highlight the prevalence of communication barriers and the limited degree of confidence in government authorities and contact with support organizations this population faces. Notably, survey respondents expressed a strong intention to report concerns/issues to authorities while simultaneously reporting that they lacked the knowledge to initiate such complaints. These findings call into question government responses that task the agricultural industry with addressing access and service gaps that may be more effectively addressed by government agencies and service providers. In order to improve supports and protections for migrant agricultural workers, policies and practices should be implemented that: (1) empower workers to independently access health, social, and legal protections and limit workers’ dependence on their employers when help-seeking; (2) provide avenues for increased proactive inspections, anonymous reporting, alternative housing/employment and meaningful 2-way communication with regulators so that the burden of reporting is lessened for this workforce; (3) systematically address breaches in privacy, translation, and adequate workplace injury assessments in the healthcare system. Ultimately, the COVID-19 context has put into sharper focus the complex gaps in health, social and legal services and protections for migrant agricultural workers. The close chronology of our data collection with this event can help us understand the factors that have resulted in so much tragedy among this workforce.
Sex Difference in the Association between Physical Activity and All-Cause Mortality in Ambulatory Patients with Chronic Kidney Disease
Authors: Molsted Stig , Eidemak Inge , Aadahl Mette
Published: April 2021

(1) Background: The purpose of this article was to investigate the association between self-reported physical activity (PA) and all-cause mortality in ambulatory patients with chronic kidney disease (CKD), stage 4–5 including maintenance dialysis. (2) Methods: Ambulatory patients with CKD (eGFR < 30 mL/min/1.73 m2) with conservative treatment or chronic dialysis were included. PA was assessed using the Saltin–Grimby Physical Activity Level Scale. A Cox proportional hazards regression model––adjusted for age, sex, plasma–albumin, body mass index, socioeconomic status, and treatment––was applied. (3) Results: Participants (n = 374) were followed 39 ± 15 months from entry to death or censoring. Throughout the study period of 39 months, 156 deaths (42%) were registered. Regarding physical activity, 128 (34%) of the participants were inactive, 212 (57%) were moderately active, and 34 (9%) were highly or vigorously active. Moderate PA was associated with a decreased mortality risk in women (n = 150) compared to inactivity (HR 0.27 (0.15; 0.51), p < 0.001), whereas a high/vigorous level of PA was not significantly associated with mortality risk compared to inactivity. In men (n = 224), the associations between PA levels and mortality risk were not significant. (4) Conclusions: Moderate PA was associated with reduced all-cause mortality in ambulatory women with stage 4–5 CKD with or without maintenance dialysis treatment. Physical activity was not significantly associated with mortality in men.
Mediterranean Diet Implementation to Protect against Advanced Lung Cancer Index (ALI) Rise: Study Design and Preliminary Results of a Randomised Controlled Trial
Authors: Gioxari Aristea , Tzanos Dimitrios , Kostara Christina , Papandreou Panos , Mountzios Giannis , Skouroliakou Maria
Published: April 2021

The Mediterranean diet (MD) has been inversely associated with lung cancer (LC) risk. Hereby we show the preliminary results of our prospective randomised controlled trial in inflammatory and nutritional status of LC patients after 3-month implementation of MD. In total, 30 patients with small-cell or non-small-cell LC (stages III–IV) were enrolled. They were randomly assigned either to Control group, receiving general nutritional guidelines, or the MD group, in which a personalised MD plan was provided. Medical and dietary history, anthropometrics, blood biomarkers, and circulating antioxidant vitamins were assessed. The main outcome was a significantly higher advanced lung cancer inflammation index (ALI) in patients of the control arm than those following MD (p = 0.003). In the MD group, platelets were significantly reduced at the study endpoint (p = 0.044). BMI and body fat mass remained unchanged in both arms, but serum glucose was significantly higher in control compared to MD group (p = 0.017). In conclusion, we showed for the first time that implementing a personalised MD for 3 months is promising to regulate prognostic biomarkers in advanced LC. The final results of our on-going trial will shed a light on the inflammatory, antioxidant and nutritional status of LC patients following MD.
Absence of Objective Differences between Self-Identified Addicted and Healthy Smartphone Users?
Authors: Geyer Kristoffer , Carbonell Xavier , Beranuy Marta , Calvo Fran
Published: April 2021

Smartphones are used by billions of people worldwide. However, some psychologists have argued that use of this technology is addictive, even though little research utilises objective smartphone usage records to verify this claim. We conducted an exploratory study to identify whether behavioural differences exist between those who self-identify as addicted smartphone users and those who do not. We gathered retrospective smartphone usage data from 131 Android users and asked them about their past use to compare their perception of their usage against their actual usage. We could not identify any reliable differences between the smartphone activity of those self-identified as addicted smartphone users and other users. Furthermore, smartphone scales are generally good at identifying who believes themselves to be addicted, although they do not reflect objective smartphone use. This study questions the use of self-report measures to diagnosis behavioural addictions without relevant psychopathological constructs and emphasises the need for more rigorous study to conceptualise smartphone addiction.
Relationship between Dietary Patterns and Cardiovascular Disease Risk in Korean Older Adults
Authors: Seo Ae-Rim , Hwang Tae-Yoon
Published: April 2021

Objectives: The purpose of this study was to assess the relationship between dietary patterns and the 10-year risk of cardiovascular disease (CVD) in the elderly population in Korea. Methods: Cluster analysis was conducted on the data of 1687 elderly participants (797 men and 890 women) aged ≥65 years from the 2014–2016 Korea National Health and Nutrition Examination Survey (KNHANES), using a 24-h dietary recall survey to assess dietary patterns. Dietary patterns were classified into clusters 1 (typical Korean diet), 2 (high carbohydrate diet), and 3 (healthy diet). The 10-year risk of CVD was calculated based on age, total and HDL-cholesterol levels, systolic blood pressure level, antihypertensive medication use, smoking status, and presence of diabetes. A complex sample general linear model was applied to determine the association between dietary patterns and the 10-year risk of CVD. Results: In total, 275 (33.7%), 141 (17.9%), and 381 (48.3%) men, and 207 (22.6%), 276 (30.9%), and 407(46.6%) women were included in clusters 1, 2, and 3, respectively. The 10-year risk of CVD was lower in men in cluster 3 (healthy diet) than in those in cluster 1 (typical Korean diet) (t = 2.092, p = 0.037). Additionally, the 10-year risk of CVD was lower in men who performed strength training than in those who did not (t = 3.575, p < 0.001). There were no significant differences in women. Conclusions: After adjusting for sociodemographic variables, men who consumed a healthy diet had a lower 10-year risk of CVD than those who consumed a typical Korean diet. When organizing nutrition education programs to improve dietary habits in the elderly, content on diets that consist of various food groups to prevent CVD is required. In particular, it is necessary to develop content that emphasizes the importance of healthy eating habits in men.
The Mechanism of Facultative Intracellular Parasitism of Brucella
Authors: Jiao Hanwei , Zhou Zhixiong , Li Bowen , Xiao Yu , Li Mengjuan , Zeng Hui , Guo Xiaoyi , Gu Guojing
Published: April 2021

Brucellosis is a highly prevalent zoonotic disease characterized by abortion and reproductive dysfunction in pregnant animals. Although the mortality rate of Brucellosis is low, it is harmful to human health, and also seriously affects the development of animal husbandry, tourism and international trade. Brucellosis is caused by Brucella, which is a facultative intracellular parasitic bacteria. It mainly forms Brucella-containing vacuoles (BCV) in the host cell to avoid the combination with lysosome (Lys), so as to avoid the elimination of it by the host immune system. Brucella not only has the ability to resist the phagocytic bactericidal effect, but also can make the host cells form a microenvironment which is conducive to its survival, reproduction and replication, and survive in the host cells for a long time, which eventually leads to the formation of chronic persistent infection. Brucella can proliferate and replicate in cells, evade host immune response and induce persistent infection, which are difficult problems in the treatment and prevention of Brucellosis. Therefore, the paper provides a preliminary overview of the facultative intracellular parasitic and immune escape mechanisms of Brucella, which provides a theoretical basis for the later study on the pathogenesis of Brucella.
Technical Note: Effects of Makeshift Storage in Different Liquors on Larvae of the Blowflies Calliphora vicina and Lucilia sericata (Diptera: Calliphoridae)
Authors: Niederegger Senta
Published: April 2021

Unexpected findings of forensically important insects might prompt makeshift storage in alternative liquids if the proper equipment is lacking. The assessment of whether such evidence can still be used and correctly interpreted can be difficult. In this study, the effects of using alcoholic beverages as storing agents for post-feeding larvae of Calliphora vicina and Lucilia sericata were analyzed. Larvae were killed with boiling water (HWK) or placed alive into four alcoholic liquids: two spirits, vodka and brandy, and two liquors, Jägermeister and peppermint schnapps. Storage effects were documented after one day, nine days, and one month and compared to larvae treated according to guidelines for forensic entomology. Results show that the method of killing larvae is more important than the storing medium. Storage of HWK larvae in high-alcohol/low-sugar spirits had almost negligible effects on both species, while all fresh larvae shrank significantly. High sugar contents of the beverages might additionally lead to shrinkage of larvae.
Assessing the Distribution of Exotic Egg Parasitoids of Halyomorpha halys in Europe with a Large-Scale Monitoring Program
Authors: Zapponi Livia , Tortorici Francesco , Anfora Gianfranco , Bardella Simone , Bariselli Massimo , Benvenuto Luca , Bernardinelli Iris , Butturini Alda , Caruso Stefano , Colla Ruggero , Costi Elena , Culatti Paolo , Di Bella Emanuele , Falagiarda Martina , Giovannini Lucrezia , Haye Tim , Maistrello Lara , Malossini Giorgio , Marazzi Cristina , Marianelli Leonardo , Mele Alberto , Michelon Lorenza , Moraglio Silvia Teresa , Pozzebon Alberto , Preti Michele , Salvetti Martino , Scaccini Davide , Schmidt Silvia , Szalatnay David , Roversi Pio Federico , Tavella Luciana , Tommasini Maria Grazia , Vaccari Giacomo , Zandigiacomo Pietro , Sabbatini-Peverieri Giuseppino
Published: April 2021

The brown marmorated stink bug Halyomorpha halys is an invasive agricultural pest with a worldwide distribution. Classical biological control has been identified as the most promising method to reduce the populations of H. halys. Adventive populations of two candidates for releases, Trissolcus japonicus and Trissolcus mitsukurii, have recently been detected in Europe. To assess their distribution and abundance, a large-scale survey was performed. From May to September 2019, a wide area covering northern Italy and parts of Switzerland was surveyed, highlighting the expanding distribution of both Tr. japonicus and Tr. mitsukurii. Within four years after their first detection in Europe, both species have rapidly spread into all types of habitats where H. halys is present, showing a wide distribution and continuous expansion. Both exotic Trissolcus showed high levels of parasitism rate towards H. halys, while parasitization of non-target species was a rare event. The generalist Anastatus bifasciatus was the predominant native parasitoid of H. halys, while the emergence of native scelionids from H. halys eggs was rarely observed. The presence of the hyperparasitoid Acroclisoides sinicus was also recorded. This study provided fundamental data that supported the development of the first inoculative release program of Tr. japonicus in Europe.
Diaphorina citri Genome Possesses a Complete Melatonin Biosynthesis Pathway Differentially Expressed under the Influence of the Phytopathogenic Bacterium, Candidatus Liberibacter asiaticus
Authors: Nehela Yasser , Killiny Nabil
Published: April 2021

Melatonin is synthesized from the amino acid L-tryptophan via the shikimic acid pathway and ubiquitously distributed in both prokaryotes and eukaryotes. Although most of melatonin biosynthesis genes were characterized in several plants and animal species including the insect model, Drosophila melanogaster, none of these enzymes have been identified from the Asian citrus psyllid, Diaphorina citri. We used comprehensive in silico analysis and gene expression techniques to identify the melatonin biosynthesis-related genes of D. citri and to evaluate the expression patterns of these genes within the adults of D. citri with gradient infection rates (0, 28, 34, 50, 58, and 70%) of the phytopathogenic bacterium Candidatus Liberibacter asiaticus and after the treatment with exogenous melatonin. We showed that the D. citri genome possesses six putative melatonin biosynthesis-related genes including two putative tryptophan 5-hydroxylase (DcT5H-1 and DcT5H-2), a putative aromatic amino acid decarboxylase (DcAADC), two putative arylalkylamine N-acetyltransferase (DcAANAT-1 and DcAANAT-2), and putative N-acetylserotonin O-methyltransferase (DcASMT). The infection with Ca. L. asiaticus decreased the transcript levels of all predicted genes in the adults of D. citri. Moreover, melatonin supplementation induced their expression levels in both healthy and Ca. L. asiaticus-infected psyllids. These findings confirm the association of these genes with the melatonin biosynthesis pathway.
Paliperidone Palmitate Every Three Months (PP3M) 2-Year Treatment Compliance, Effectiveness and Satisfaction Compared with Paliperidone Palmitate-Monthly (PP1M) in People with Severe Schizophrenia
Authors: Fernández-Miranda Juan J. , Díaz-Fernández Silvia , De Berardis Domenico , López-Muñoz Francisco
Published: April 2021

Paliperidone palmitate every three months (PP3M) is expected to facilitate patient’s treatment compliance and satisfaction. The objective here was to compare PP3M treatment compliance and satisfaction, effectiveness and tolerability, with paliperidone palmitate-monthly (PP1M) in patients with severe schizophrenia. A 24-month prospective, open-label study of patients with severe schizophrenia treated with PP3M after at least 2 years of stabilization with PP1M (n = 84) was carried out. Treatment satisfaction was assessed with the Treatment Satisfaction Questionnaire for Medication (TSQM) and with a Visual Analogue Scale (VAS). Effectiveness was measured with psychiatric hospital admissions and the Clinical Global Impression-Severity (CGI-S) scale. Tolerability assessments included laboratory tests, weight and adverse effects. Reasons for treatment discontinuation were recorded. CGI-S significantly improved after 24 months. Three patients changed back to PP1M due to adverse effects, and four were hospitalized. There were neither abandoning nor significant changes in weight or biological parameters, and lower incidence of side effects, with PP3M treatment. TSQM and VAS scales increased. No differences were found related to doses. Apart from somewhat improvement in treatment adherence, effectiveness, and tolerability, patients with severe schizophrenia lengthy treated with PP1M showed more satisfaction with PP3M, even those who needed high doses to get clinical stabilization.
Bone Marrow Mastocytosis: A Diagnostic Challenge
Authors: Zanotti Roberta , Tanasi Ilaria , Bernardelli Andrea , Orsolini Giovanni , Bonadonna Patrizia
Published: April 2021

Bone marrow mastocytosis (BMM) represents a provisional, indolent subvariant of systemic mastocytosis (SM). Utilizing WHO criteria, BMM requires bone marrow (BM) involvement and the absence of mastocytosis skin lesions. BMM is characterized by male sex prevalence, a slight increase of serum tryptase levels, low BM mast cells (MC) burden, and an indolent clinical course. BMM shows a strong correlation with severe anaphylaxis, mainly due to an IgE-mediated allergy to bee or wasp venom and, less frequently, to unexplained (idiopathic) anaphylaxis. Furthermore, BMM is often associated with osteoporosis which could be the only presenting symptom of the disease. BMM is an undervalued disease as serum tryptase levels are not routinely measured in the presence of unexplained osteoporosis or anaphylaxis. Moreover, BMM patients are often symptom-free except for severe allergic reactions. These factors, along with typical low BM MCs infiltration, may contribute to physicians overlooking BMM diagnosis, especially in medical centers that lack appropriately sensitive diagnostic techniques. This review highlights the need for a correct diagnostic pathway to diagnose BMM in patients with suspected symptoms but lacking typical skin lesions, even in the case of normal serum tryptase levels. Early diagnosis may prevent potential life-threatening anaphylaxis or severe skeletal complications.
Big Data in Studying Acute Pain and Regional Anesthesia
Authors: Müller-Wirtz Lukas M. , Volk Thomas
Published: April 2021

The digital transformation of healthcare is advancing, leading to an increasing availability of clinical data for research. Perioperative big data initiatives were established to monitor treatment quality and benchmark outcomes. However, big data analyses have long exceeded the status of pure quality surveillance instruments. Large retrospective studies nowadays often represent the first approach to new questions in clinical research and pave the way for more expensive and resource intensive prospective trials. As a consequence, the utilization of big data in acute pain and regional anesthesia research has considerably increased over the last decade. Multicentric clinical registries and administrative databases (e.g., healthcare claims databases) have collected millions of cases until today, on which basis several important research questions were approached. In acute pain research, big data was used to assess postoperative pain outcomes, opioid utilization, and the efficiency of multimodal pain management strategies. In regional anesthesia, adverse events and potential benefits of regional anesthesia on postoperative morbidity and mortality were evaluated. This article provides a narrative review on the growing importance of big data for research in acute postoperative pain and regional anesthesia.
Impact of Different JAK Inhibitors and Methotrexate on Lymphocyte Proliferation and DNA Damage
Authors: Reddig Annika , Voss Linda , Guttek Karina , Roggenbuck Dirk , Feist Eugen , Reinhold Dirk
Published: April 2021

Janus kinase inhibitors (JAKis) represent a new strategy in rheumatoid arthritis (RA) therapy. Still, data directly comparing different JAKis are rare. In the present in vitro study, we investigated the immunomodulatory potential of four JAKis (tofacitinib, baricitinib, upadacitinib, and filgotinib) currently approved for RA treatment by the European Medicines Agency. Increasing concentrations of JAKi or methotrexate, conventionally used in RA therapy, were either added to freshly mitogen-stimulated or preactivated peripheral blood mononuclear cells (PBMC), isolated from healthy volunteers. A comparable, dose-dependent inhibition of lymphocyte proliferation was observed in samples treated with tofacitinib, baricitinib, and upadacitinib, while dosage of filgotinib had to be two orders of magnitude higher. In contrast, antiproliferative effects were strongly attenuated when JAKi were added to preactivated PBMCs. High dosage of upadacitinib and filgotinib also affected cell viability. Further, analyses of DNA double-strand break markers γH2AX and 53BP1 indicated an enhanced level of DNA damage in cells incubated with high concentrations of filgotinib and a dose-dependent reduction in clearance of radiation-induced γH2AX foci in the presence of tofacitinib or baricitinib. Thereby, our study demonstrated a broad comparability of immunomodulatory effects induced by different JAKi and provided first indications, that (pan)JAKi may impair DNA damage repair in irradiated PBMCs.
Plasma Concentrations of Matrilysins MMP-7 and MMP-26 as Diagnostic Biomarkers in Breast Cancer
Authors: Piskór Barbara Maria , Przylipiak Andrzej , Dąbrowska Emilia , Sidorkiewicz Iwona , Niczyporuk Marek , Szmitkowski Maciej , Ławicki Sławomir
Published: April 2021

Metalloproteinases (MMPs) are a group of proteolytic enzymes involved in the maintenance of a proper structure of extracellular matrix (ECM). Matrilysins (MMP-7 and MMP-26) are members of the MMPs group that show promise as potential breast cancer (BC) markers. The aim of the study was to evaluate plasma levels of MMP-7, MMP-26 and CA 15-3 individually and in combination and assess the diagnostic utility of studied matrilysins in patients with BC. The study group consisted of 120 patients with BC, and the control group consisted of 40 subjects with benign breast cancer and 40 healthy women. Concentrations of MMP-7 and MMP-26 were determined by enzyme-linked immunosorbent assay, and CA 15-3 by chemiluminescent microparticle immunoassay. Plasma levels of MMP-7 were significantly higher in the BC group than in the control group. Concentrations of MMP-26 and CA 15-3 were highest in stages II and IV of the disease. The highest diagnostic sensitivity was observed in stages III and IV BC for the combination of all tested markers (92.5%). The highest diagnostic specificity was noted for all tested parameters combined in the BC group (95.0%). The area under the receiver operating characteristic (ROC) curve (AUC) for the combination of markers (MMP-7+MMP-26+CA 15-3) was the largest (0.9138) in stages III and IV. Individual marker analysis showed that MMP-7 had the highest AUC (0.8894) in advanced stages of the disease. Study results indicate that MMP-7 could be used as an additional marker that would improve the diagnostic utility of CA 15-3 in early stages of BC. Therefore, the combined assessment of MMP-7 and MMP-26 with CA 15-3 might be useful in determining disease progression. Further studies are needed to evaluate whether matrilysins show promise as potential markers for improving the diagnosis of BC.
Novel Functional Indices of Masticatory Muscle Activity
Authors: Ginszt Michał , Zieliński Grzegorz
Published: April 2021

The aim of the present study was to analyze novel functional indices of masticatory muscle activity and compare them to existing and commonly used indices in patients with temporomandibular disorders (TMDs) and healthy adults. Based on the Research Diagnostic Criteria for Temporomandibular Disorders, 78 adult women qualified for the study. Subjects were divided into two groups: diagnosed TMDs (n = 36; mean age: 23.4 ± 2.6 years) and healthy adults (n = 42; mean age: 22.4 ± 2.3 years). Measurements of the bioelectric activity of the temporalis anterior (TA), superficial masseter (MM), and anterior bellies of the digastric muscle (DA) were carried out using the BioEMG III ™. Functional Clenching (FCI) and Functional Opening (FOI) indices were obtained as the ratio of the difference between the mean muscle root mean square (RMS) potentials during functional activity, including clenching (CL) and opening (MMO), and mean muscle resting (REST) potentials. Next, based on FCI and FOI indices, the Functional Clenching Activity Index (FCAI), Functional Clenching Symmetry Index (FCSI), and Functional Opening Symmetry Index (FOSI) were obtained. The statistical analysis showed significant differences in activity index left-sided (AcIL) and Activity index both-sided (AcItot) between TMDs and healthy women during rest measurements. The significant differences between both groups were noted in terms of all Functional Clenching Indices except Functional Clenching Index for MM right-sided (FCIMM-R). In all analyzed FCI indices, the control group showed higher values compared to the TMDs. Moreover, a significant difference between TMDs and controls was observed within Functional Clenching Activity Index left-sided (FCAIL) (14.56 vs. −0.45, p = 0.01). Both functional indices, and asymmetry (AsI) and activity (AcI) indices seem to be reliable in assessing symmetry and activity within masticatory muscles. Further studies should be performed to verify the effectiveness and suitability of the assessment of masticatory muscles using functional indices.
Changes in Back Pain Scores after Bariatric Surgery in Obese Patients: A Systematic Review and Meta-Analysis
Authors: Koremans Froukje W. , Chen Xiaolong , Das Abhirup , Diwan Ashish D.
Published: April 2021

Bariatric surgery produces significant and quantifiable reductions in back pain. However, there is a lack of information on the association of weight changes after bariatric surgery with changes in pain score. We aim to evaluate the impact of bariatric surgery on back pain in obese patients and to address the association between changes in body mass index (BMI) and pain score. In obese patients eligible for bariatric surgery, the changes in pre- and post-operative pain scores, assessed by the Numeric Rating Pain Scale (NPS) or Visual Analogue Scale (VAS), were considered as primary outcomes. Mean difference (MD) and their 95% confidence intervals (CI) were evaluated. Eight cohort studies were included in the analysis of 298 obese patients undergoing bariatric surgery. All studies showed a reduction in back pain, with a mean change of −2.9 points in NPS and of −3.8 cm in VAS. There was a significant reduction in back pain (NPS: (MD = −3.49) (95% CI = −3.86, −3.12); VAS: MD = −3.75, (95% CI = −4.13, −3.37)) and BMI (MD = −12.93, (95% CI = −13.61, −12.24)) following bariatric surgery. No significant relationship between BMI change and decrease in clinical scores could be established. However, it was evident that bariatric surgery had a significant effect on back pain scores in severely obese patients. Ideally, a prospective study including spinal imaging, inflammatory markers, a longer follow-up period, and larger study groups with a randomized control group needs to be performed.
Statin Use Is Associated with a Decreased Risk of Mortality among Patients with COVID-19
Authors: Wu Chieh-Chen , Lee An-Jen , Su Chun-Hsien , Huang Chu-Ya , Islam Md. Mohaimenul , Weng Yung-Ching
Published: April 2021

Background: Recent epidemiological studies remain controversial regarding the association between statin use and reducing the risk of mortality among individuals with COVID-19. Objective: The objective of this study was to clarify the association between statin use and the risk of mortality among patients with COVID-19. Methods: We conducted a systematic articles search of online databases (PubMed, EMBASE, Scopus, and Web of Science) between 1 February 2020 and 20 February 2021, with no restriction on language. The following search terms were used: “Statins” and “COVID-19 mortality or COVID19 mortality or SARS-CoV-2 related mortality”. Two authors individually examined all articles and followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines for study inclusion and exclusion. The overall risk ratio (RRs) with 95% confidence interval (CI) was calculated to show the strength of the association and the heterogeneity among the studies was presented Q and I2 statistic. Results: Twenty-eight studies were assessed for eligibility and 22 studies met the inclusion criteria. Statin use was associated with a significantly decreased risk of mortality among patients with COVID-19 (RR adjusted = 0.64; 95% CI: 0.57–0.72, p < 0.001). Moreover, statin use both before and after the admission was associated with lowering the risk of mortality among the COVID-19 patients (RR adjusted;before = 0.69; 95% CI: 0.56–0.84, p < 0.001 and RR adjusted;after = 0.57; 95% CI: 0.54–0.60, p < 0.001). Conclusion: This comprehensive study showed that statin use is associated with a decreased risk of mortality among individuals with COVID-19. A randomized control trial is needed to confirm and refute the association between them.
Alternative Procedure to Individual Nasal Pressure Titration for Sleep Apnea
Authors: Farré Ramon , Gozal David , Montserrat Josep M.
Published: April 2021

In the treatment of obstructive sleep apnea (OSA), the current standard of “CPAP titration” in the laboratory or at home is a resource demanding and costly approach that, in developed economies, markedly augments healthcare costs and in low resource economies precludes access to care altogether. Here, we discuss that current guidelines for titration of CPAP could be obviated by taking a different route that in many ways is similar to the institution of treatment in many other medical conditions. To this effect, we present novel population based data from 16,780 patients, showing that after individualized and labor-intensive and expensive CPAP titration, 86.4% of OSA patients are treated with nasal pressure settings within the range of 9 ± 2 cmH2O, and review the literature to justify the potential adoption of a standard therapeutic CPAP setting as the initial intervention which would be subsequently followed by any necessary adjustments in only a minority of patients who would not derive the necessary benefit from such standardized intervention. Assuming an 80–85% success rate as derived from our analyses, our personal view if extensively adopted could radically reduce healthcare costs and enable markedly improve access to diagnostics.
Development of a Shape Replicating Draping Unit for Continuous Layup of Unidirectional Non-Crimp Fabrics on Complex Surface Geometries
Authors: Denkena Berend , Schmidt Carsten , Werner Simon , Schwittay Dietmar
Published: April 2021

The manufacturing of large-scale structural components is still dominated by manual labor in many sectors of the modern composite industry. Efforts are being made to establish an automated layup technology for complex structural elements. Processing dry non-crimp fiber fabrics (NCF) offers great cost opportunities and high deposition rates, compared to prepreg-based technologies like automated fiber placement (AFP). Here, the fabric architecture is considered during the draping of the plane textile on curved surfaces. In this paper, the development of a draping unit for balancing fabric tension and consolidating continuously across the layup width is presented. We introduce a geometrical process model to achieve a fabric-friendly draping of the used unidirectional NCF. The shape of the resulting draping front depends on the surface geometry, the shearing of the previously laid-up textile, and the positioning of the material feed. To consolidate the fabric at the altering draping front in an automated layup process, the shape of the continuous consolidation element can be controlled by the elongation of serial soft actuators, manipulated by parallel robot kinematics. The shape replication ability of the draping unit is promising for the implementation of a continuous, fabric-friendly draping process for complex surface geometries.
Impacts of Agricultural Land Reclamation on Soil Nutrient Contents, Pools, Stoichiometry, and Their Relationship to Oat Growth on the East China Coast
Authors: Xie Xuefeng , Xiang Qi , Wu Tao , Zhu Ming , Xu Fei , Xu Yan , Pu Lijie
Published: April 2021

Agricultural land reclamation of coastal tidal land (CTL) with organic amendments may modulate the soil properties, and therefore promote crop growth. However, the linkages between soil nutrient contents, pools, stoichiometry, and crop growth under the supplement of organic amendments in CTL is limited. In this study, six treatments including the control (CK), organic manure (OM), polyacrylamide plus organic manure (PAM + OM), straw mulching plus organic manure (SM + OM), buried straw plus organic manure (BS + OM), and bio-organic manure plus organic manure (BM + OM) were conducted to explore these linkages in newly reclaimed CTL in Jiangsu Province, eastern China. The results showed that the application of different soil reclamation treatments increased soil nutrient contents, pools, and modulated their stoichiometric ratio, which thus promoted the growth of oat. Soil under all reclamation treatments increased the contents of surface soil organic carbon (SOC), total nitrogen (TN), and total phosphorus (TP), and the BM + OM treatment had the highest increase, which increased by 11.7–182.4%, 24.3–85.7%, 3.2–29.4%, respectively. The highest soil C pools were observed in the oat heading stage (36.67–41.34 Mg C ha−1), whereas the soil N and P pools were more stable during the oat growth period. Similarly, the highest surface soil C/N and C/P were observed in the oat heading stage (11.23–14.67 and 8.97–14.21), whereas the N/P in surface soil increased compared with the CK treatment during the oat growth period, with the exception of the filling stage. Land reclamation treatments significantly promoted oat growth by changing soil C, N, and P contents, pools, and stoichiometry, among which soil SOC, TN, TP, C/P, and N/P are more closely related to oat growth (p < 0.05).
Ozone Response of Leaf Physiological and Stomatal Characteristics in Brassica juncea L. at Supraoptimal Temperatures
Authors: Lee Jong Kyu , Kwak Myeong Ja , Park Sang Hee , Kim Han Dong , Lim Yea Ji , Jeong Su Gyeong , Choi Yun Soo , Woo Su Young
Published: April 2021

Plants are affected by the features of their surrounding environment, such as climate change and air pollution caused by anthropogenic activities. In particular, agricultural production is highly sensitive to environmental characteristics. Since no environmental factor is independent, the interactive effects of these factors on plants are essential for agricultural production. In this context, the interactive effects of ozone (O3) and supraoptimal temperatures remain unclear. Here, we investigated the physiological and stomatal characteristics of leaf mustard (Brassica juncea L.) in the presence of charcoal-filtered (target concentration, 10 ppb) and elevated (target concentration, 120 ppb) O3 concentrations and/or optimal (22/20 °C day/night) and supraoptimal temperatures (27/25 °C). Regarding physiological characteristics, the maximum rate of electron transport and triose phosphate use significantly decreased in the presence of elevated O3 at a supraoptimal temperature (OT conditions) compared with those in the presence of elevated O3 at an optimal temperature (O conditions). Total chlorophyll content was also significantly affected by supraoptimal temperature and elevated O3. The chlorophyll a/b ratio significantly reduced under OT conditions compared to C condition at 7 days after the beginning of exposure (DAE). Regarding stomatal characteristics, there was no significant difference in stomatal pore area between O and OT conditions, but stomatal density under OT conditions was significantly increased compared with that under O conditions. At 14 DAE, the levels of superoxide (O2-), which is a reactive oxygen species, were significantly increased under OT conditions compared with those under O conditions. Furthermore, leaf weight was significantly reduced under OT conditions compared with that under O conditions. Collectively, these results indicate that temperature is a key driver of the O3 response of B. juncea via changes in leaf physiological and stomatal characteristics.
Effects of Land-Use Change on Soil Functionality and Biodiversity: Toward Sustainable Planning of New Vineyards
Authors: Gagnarli Elena , Valboa Giuseppe , Vignozzi Nadia , Goggioli Donatella , Guidi Silvia , Tarchi Franca , Corino Lorenzo , Simoni Sauro
Published: April 2021

Sustainable agriculture largely depends on soil biodiversity and requires efficient methods to assess the effectiveness of agronomic planning. Knowledge of the landscape and relative pedosite is enriched by data on the soil microarthropod community, which represent useful bio-indicators for early soil-quality detection in land-use change (LUC). In the hilly Maremma region of Grosseto, Italy, two areas, a >10ys meadow converted into a vineyard and an old biodynamic vineyard (no-LUC), were selected for evaluating the LUC effect. For maintaining soil vitality and ecosystem services by meadow, the vineyard was planted and cultivated using criteria of the patented “Corino method”. The aim was to evaluate the LUC impact, within one year, by assessing parameters characterizing soil properties and soil microarthropod communities after the vineyard was planted. The adopted preservative method in the new vineyards did not show a detrimental impact on the biodiversity of soil microarthropods, and in particular, additional mulching contributed to a quick recovery from soil stress due to working the plantation. In the short term, the adopted agricultural context confirmed that the targeted objectives preserved the soil quality and functionality.
Assessing the Impact of Urban Expansion on Surrounding Forested Landscape Connectivity across Space and Time
Authors: Ren Zhouqiao , He Jianhua , Yue Qiaobing
Published: April 2021

Landscape connectivity is important for all organisms as it directly affects population dynamics. Yet, rapid urbanization has caused serious landscape fragmentation, which is the primary contributor of species extinctions worldwide. Previous studies have mostly used spatial snap-shots to evaluate the impact of urban expansion on landscape connectivity. However, the interactions among habitats over time in dynamic landscapes have been largely ignored. Here, we demonstrated that overlooking temporal connectivity can lead to the overestimation of the impact of urban expansion. How much greater the overestimation is depends on the amount of net habitat loss. Moreover, we showed that landscape connectivity may have a delayed response to urban expansion. Our analysis shifts the way to understand the ecological consequences of urban expansion. Our framework can guide sustainable urban development and can be inspiring to conservation practices under other contexts (e.g., climate change).
Modeling Future Land Use Development: A Lithuanian Case
Authors: Mozgeris Gintautas , Juknelienė Daiva
Published: April 2021

Effective management decisions regarding greenhouse gas (GHG) emissions may be hampered by the lack of scientific tools for modeling future land use change. This study addresses methodological principles for land use development scenario modeling assumed for use in processes of GHG accounting and management. Associated land use policy implications in Lithuania are also discussed. Data on land uses, available from the National Forest Inventory (NFI) and collected for GHG accounting from the land use, land use change and forestry (LULUCF) sector in the country, as well as freely available geographic information, were tested as an input for modeling land use development in the country. The modeling was implemented using the TerrSet Land Change Modeler. Calibration of the modeling approach using historical land use data indicated that land use types important for GHG management in the LULUCF sector were predicted with an accuracy above 80% during a five-year period into the future, while the prediction accuracy for forest and built-up land was 96% or more. Based on several land management scenarios tested, it was predicted that the LULUCF sector in Lithuania will accumulate CO2, with the forest land use type contributing most to CO2 absorption. Key measures to improve the GHG balance and carbon stock changes were suggested to be the afforestation of abandoned or unused agricultural land and prevention of the conversion of grassland into producing land.
Fine Root Traits of Pinus koraiensis Varied with Soil Cation Exchange Capacity in Natural Forests
Authors: Jia Shuxia , Li Xingpeng , Sun Wensheng , Wang Qian , Liu Hongwen , Zhou Chunyan , Zhang Weina , Li Feng
Published: April 2021

Adaptation of fine root plasticity to soil nutrients heterogeneity in natural forest ecosystems has not been well explored. The study aimed to determine seasonal variations of fine root traits in Pinus koraiensis natural forests and explore the relationship between fine root traits and soil properties. Root and soil samples were collected from the coniferous broad-leaved mixed forest (BP), monospecific P. koraiensis forest (MP), and coniferous mixed forest (CP). Soil available phosphorus (P) content for MP was 31.7% and 39.8% lower than for BP and CP. Soil cation exchange capacity (CEC) for MP was lower by 23.5% and 27.2% than for BP and CP, respectively. In July, specific root lengths and root surface areas for BP and CP (mixed forests) were significantly higher than for MP (monospecific forest). A structural equation model showed that CEC had a significant effect on fine root traits. Root diameter, root volume density, and root surface area density were negatively correlated with CEC. Fine root traits plasticity of P. koraiensis are closely related to soil available nutrient contents, CEC, and species composition at the ecosystem level.