bluuebunny/crossref_metadata_2025 huggingface dataset:
Inter-study and time-dependent variability of metabolite abundance in cultured red blood cells
Authors: Tewari Shivendra G. , Rajaram Krithika , Swift Russell P. , Kwan Bobby , Reifman Jaques , Prigge Sean T. , Wallqvist Anders
Published: July 2021

Background

Cultured human red blood cells (RBCs) provide a powerful ex vivo assay platform to study blood-stage malaria infection and propagation. In recent years, high-resolution metabolomic methods have quantified hundreds of metabolites from parasite-infected RBC cultures under a variety of perturbations. In this context, the corresponding control samples of the uninfected culture systems can also be used to examine the effects of these perturbations on RBC metabolism itself and their dependence on blood donors (inter-study variations).

Methods

Time-course datasets from five independent studies were generated and analysed, maintaining uninfected RBCs (uRBC) at 2% haematocrit for 48 h under conditions originally designed for parasite cultures. Using identical experimental protocols, quadruplicate samples were collected at six time points, and global metabolomics were employed on the pellet fraction of the uRBC cultures. In total, ~ 500 metabolites were examined across each dataset to quantify inter-study variability in RBC metabolism, and metabolic network modelling augmented the analyses to characterize the metabolic state and fluxes of the RBCs.

Results

To minimize inter-study variations unrelated to RBC metabolism, an internal standard metabolite (phosphatidylethanolamine C18:0/20:4) was identified with minimal variation in abundance over time and across all the samples of each dataset to normalize the data. Although the bulk of the normalized data showed a high degree of inter-study consistency, changes and variations in metabolite levels from individual donors were noted. Thus, a total of 24 metabolites were associated with significant variation in the 48-h culture time window, with the largest variations involving metabolites in glycolysis and synthesis of glutathione. Metabolic network analysis was used to identify the production of superoxide radicals in cultured RBCs as countered by the activity of glutathione oxidoreductase and synthesis of reducing equivalents via the pentose phosphate pathway. Peptide degradation occurred at a rate that is comparable with central carbon fluxes, consistent with active degradation of methaemoglobin, processes also commonly associated with storage lesions in RBCs.

Conclusions

The bulk of the data showed high inter-study consistency. The collected data, quantification of an expected abundance variation of RBC metabolites, and characterization of a subset of highly variable metabolites in the RBCs will help in identifying non-specific changes in metabolic abundances that may obscure accurate metabolomic profiling of Plasmodium falciparum and other blood-borne pathogens.
Associations of park access, park use and physical activity in parks with wellbeing in an Asian urban environment: a cross-sectional study
Authors: Petrunoff Nicholas A. , Yi Ng Xian , Dickens Borame , Sia Angelia , Koo Joel , Cook Alex R. , Lin Wee Hwee , Ying Lu , Hsing Ann W. , van Dam Rob M. , Müller-Riemenschneider Falk
Published: July 2021

Background

Relationships between park access, park use, and wellbeing remain poorly understood. The objectives of this study were to investigate: (1) perceived and objective park access in relation to park use and physical activity in parks; and; (2) perceived and objective park access, park use and physical activity in parks and their associations with wellbeing.

Methods

An interviewer-assisted survey collected data on perceived time to walk to parks, park use time, park physical activity time and wellbeing (using a scale containing nine domains) amongst adult participants of the Singapore Multi-Ethnic Cohort. Geospatial maps of parks and the “walkable” street networks were created for the city-state of Singapore to objectively determine distances to accessible points on park boundaries. Multiple linear regression models estimated the importance of park access to park use and associations of park access and park use with wellbeing, adjusting for potential confounders.

Results

Participants’ (n = 3435) average age was 48.8 years (SD, 12.8), 44.8% were male and 72.6% were of Chinese ethnicity. Better perceived but not true park access was significantly associated with greater park use. Park access (perceived or true) was not associated with physical activity time in parks. Greater participant park time and physical activity time in parks were associated with higher wellbeing scores (p < 0.001). The differences in wellbeing scores between the reference groups, who spent negligible time in parks, and the highest quartiles of time in parks (10.8 h/month) and physical activity in parks (8.3 h/month) were 3.2 (95% CI 2.1–4.4) and 4.2 (95% CI 4.1–6.3) points out of 100 respectively. These associations were similar for most domains of wellbeing, with clear dose-response relationships.

Conclusions

While perceived park access was strongly associated with park use and well-being, true park access was not, and neither park access measure was associated with park physical activity. Future studies could investigate the influence of park attributes on park use, physical activity in parks and wellbeing. The consistent associations of park use and particularly physical activity in parks with wellbeing suggest that promoting park use, and especially physical activity in parks, is a promising strategy for improving wellbeing in urban settings.
Is there a relationship between psoriasis and hepatitis C? A meta-analysis and bioinformatics investigation
Authors: Liu Yong , Cui Sheng Nan , Duan Meng Yao , Dou Zhi Li , Li Yi Zhen , Liu Yi Xing , Xia Ye , Zhang Jia Wei , Yan Xiao Ning , Han Dong Ran
Published: July 2021

Background

The relationship between psoriasis and hepatitis C was previously controversial, so our purpose is to investigate this connection.

Methods

We conducted a systematic review of the case–control, cross-sectional and cohort studies examining the association between psoriasis and hepatitis C in PubMed, EMBASE and Cochrane library databases and investigated the overlapping genes between psoriasis targets and hepatitis C targets using bioinformatics analysis. Based on overlapping genes and hub nodes, we also constructed the protein–protein interaction (PPI) network and module respectively, followed by the pathway enrichment analysis.

Results

We included 11 publications that reported a total of 11 studies (8 cross-sectional and 3 case–control). The case–control and cross-sectional studies included 25,047 psoriasis patients and 4,091,631 controls in total. Psoriasis was associated with a significant increase of prevalent hepatitis C (OR 1.72; 95% confidence interval [CI] (1.17–2.52)). A total of 389 significant genes were common to both hepatitis C and psoriasis, which mainly involved IL6, TNF, IL10, ALB, STAT3 and CXCL8. The module and pathway enrichment analyses showed that the common genes had the potential to influence varieties of biological pathways, including the inflammatory response, cytokine activity, cytokine–cytokine receptor interaction, Toll-like receptor signaling pathway, which play an important role in the pathogenesis of hepatitis C and psoriasis.

Conclusion

Patients with psoriasis display increased prevalence of hepatitis C and the basic related mechanisms between hepatitis C and psoriasis had been preliminarily clarified.
Effectiveness of bazedoxifene in preventing glucocorticoid-induced bone loss in rheumatoid arthritis patients
Authors: Cho Soo-Kyung , Kim Hyoungyoung , Lee Jiyoung , Nam Eunwoo , Lee Seunghun , Choi Yun Young , Sung Yoon-Kyoung
Published: July 2021

Objective

To evaluate the effectiveness of bazedoxifene in preventing bone loss in patients with rheumatoid arthritis (RA) receiving low-dose glucocorticoids (GCs).

Methods

In this randomized, controlled, open-label study, we assigned postmenopausal women with osteopenia who had been receiving low-dose GCs for RA to two groups: a group receiving bazedoxifene (20 mg/day) with elemental calcium 1200 mg and vitamin D 800 IU daily (bazedoxifene group) and a group receiving the same doses of calcium and vitamin D only (control group). As primary outcome, bone mineral density (BMD) change in the lumbar spine (L-spine) from baseline to 48 weeks was assessed. Changes in BMD in the femur, trabecular bone score, bone turnover markers, and development of fracture were assessed as secondary outcomes. For intention-to-treat analysis, 20 completed data sets were created by applying multiple imputations by chained equations.

Results

A total of 114 patients (57 patients in each group) were recruited. A significant increase in L-spine BMD (0.015 g/cm2, P = 0.007) was observed in the bazedoxifene group, and the increase was significantly higher than in the control group (0.013, 95% CI 0.0003–0.026, P = 0.047). Reductions in bone turnover markers in the bazedoxifene group were significantly greater than in the control group. Only one fracture was observed in the bazedoxifene group, while four fractures developed in the control group.

Conclusion

In postmenopausal patients with RA receiving low-dose GCs, bazedoxifene improved BMD and reduced bone turnover markers. However, the change in BMD did not exceed the least significant change.

Trial registration

ClinicalTrials.gov, NCT02602704.
Mean value of B-mode optic nerve sheath diameter as an indicator of increased intracranial pressure: a systematic review and meta-analysis
Authors: Montorfano Lisandro , Yu Qian , Bordes Stephen J. , Sivanushanthan Shankarapryan , Rosenthal Raul J. , Montorfano Miguel
Published: July 2021

Objectives

Timely diagnosis and treatment of increased intracranial pressure can decrease morbidity and prevent mortality. The present meta-analysis aims to determine the mean value of the ONSD measured in patients with various elevated ICP etiologies under different clinical settings, as well as comparing the value of ONSD between patients with and without elevated ICP.

Methods

This meta-analysis complied with the Preferred Reporting Items for Systematic Reviews and Meta-analysis Statement8. PubMed, Embase, and Cochrane Library were searched to identify ONSD measured by US for patients with increased ICP from establishment to October 2020.

Results

A total of 779 patients with elevated ICP among 22 studies were included in the present meta-analysis. Studies were published between 2003 and 2020. Eighteen were comparative (18/22, 81.8%), and four were single-armed study (4/22, 18.2%). Twenty were prospective studies (20/22, 90.9%). There was moderate-to-high heterogeneity based on the prediction ellipse area and variance logit of sensitivity and specificity.

Conclusions

The mean value of the ONSD among patients diagnosed with increased ICP was 5.82 mm (95% CI 5.58–6.06 mm). Variations were observed based on etiology of intracranial hypertension, clinical settings where ONSD was measured, and standards for diagnosing intracranial hypertension. The US-ONSD among patient with elevated ICP was significantly higher than the normal control. Although a cut-off value is not clearly determined, these mean values can be implemented to evaluate the sensitivity and specificity of US-ONSD in diagnosing intracranial hypertension in future studies.
Clinical value of 3'-deoxy-3'-[18F]fluorothymidine-positron emission tomography for diagnosis, staging and assessing therapy response in lung cancer
Authors: Alwadani Bandar , Dall’Angelo Sergio , Fleming Ian N.
Published: July 2021

Lung cancer has the highest mortality rate of any tumour type. The main driver of lung tumour growth and development is uncontrolled cellular proliferation. Poor patient outcomes are partly the result of the limited range of effective anti-cancer therapies available and partly due to the limited accuracy of biomarkers to report on cell proliferation rates in patients. Accordingly, accurate methods of diagnosing, staging and assessing response to therapy are crucial to improve patient outcomes. One effective way of assessing cell proliferation is to employ non-invasive evaluation using 3'-deoxy-3'-[18F]fluorothymidine ([18F]FLT) positron emission tomography [18F]FLT-PET. [18F]FLT, unlike the most commonly used PET tracer [18F]fluorodeoxyglucose ([18F]FDG), can specifically report on cell proliferation and does not accumulate in inflammatory cells. Therefore, this radiotracer could exhibit higher specificity in diagnosis and staging, along with more accurate monitoring of therapy response at early stages in the treatment cycle. This review summarises and evaluates published studies on the clinical use of [18F]FLT to diagnose, stage and assess response to therapy in lung cancer.
A critical review on altmetrics: can we measure the social impact factor?
Authors: García-Villar Cristina
Published: July 2021

Altmetrics measure the digital attention received by a research output. They allow us to gauge the immediate social impact of an article by taking real-time measurements of how it circulates in the Internet. While there are several companies offering attention scores, the most extensive are Altmetric.com (Altmetric Attention Score—AAS) and Plum X (Plum Print). As this is an emerging topic, many medical specialities have tried to establish if there is a relationship between an article’s altmetric data and the citations it subsequently receives. The results have varied depending on the research field. In radiology, the social network most used is Twitter and the subspeciality with the highest AAS is neuroimaging. This article will review the process involved from the start when an article is published through to finally obtaining its altmetric score. It will also address the relationship between altmetrics and more traditional approaches focusing on citations in radiology and will discuss the advantages and limitations of these new impact indicators.
Appearance of the levator ani muscle subdivisions on 3D transperineal ultrasound
Authors: Manzini Claudia , van den Noort Frieda , Grob Anique T. M. , Withagen Mariëlla I. J. , Slump Cornelis H. , van der Vaart C. Huub
Published: July 2021

Background

The levator ani muscle (LAM) consists of different subdivisions, which play a specific role in the pelvic floor mechanics. The aim of this study is to identify and describe the appearance of these subdivisions on 3-Dimensional (3D) transperineal ultrasound (TPUS). To do so, a study designed in three phases was performed in which twenty 3D TPUS scans of vaginally nulliparous women were assessed. The first phase was aimed at getting acquainted with the anatomy of the LAM subdivisions and its appearance on TPUS: relevant literature was consulted, and the TPUS scan of one patient was analyzed to identify the puborectal, iliococcygeal, puboperineal, pubovaginal, and puboanal muscle. In the second phase, the five LAM subdivisions and the pubic bone and external sphincter, used as reference structures, were manually segmented in volume data obtained from five nulliparous women at rest. In the third phase, intra- and inter-observer reproducibility were assessed on twenty TPUS scans by measuring the Dice Similarity Index (DSI).

Results

The mean inter-observer and median intra-observer DSI values (with interquartile range) were: puborectal 0.83 (0.13)/0.83 (0.10), puboanal 0.70 (0.16)/0.79 (0.09), iliococcygeal 0.73 (0.14)/0.79 (0.10), puboperineal 0.63 (0.25)/0.75 (0.22), pubovaginal muscle 0.62 (0.22)/0.71 (0.16), and the external sphincter 0.81 (0.12)/0.89 (0.03).

Conclusion

Our results show that the LAM subdivisions of nulliparous women can be reproducibly identified on 3D TPUS data.
High-efficiency c-Myc-mediated induction of functional hepatoblasts from the human umbilical cord mesenchymal stem cells
Authors: Deng Jie , Luo Kai , Xu Pengchao , Jiang Qingyuan , Wang Yuan , Yao Yunqi , Chen Xiaolei , Cheng Fuyi , Xie Dan , Deng Hongxin
Published: July 2021

Background

Direct reprogramming of human fibroblasts to hepatocyte-like cells was proposed to generate large-scale functional hepatocytes demanded by liver tissue engineering. However, the difficulty in obtaining large quantities of human fibroblasts greatly restricted the extensive implementation of this approach. Meanwhile, human umbilical cord mesenchymal stem cells (HUMSCs) are the preferred cell source for HLCs with the advantages of limited ethical concerns, easy accessibility, and propagation in vitro. However, no direct reprogramming protocol for converting HUMSCs to hepatoblast-like cells (HLCs) has been reported.

Methods

HLCs were successfully generated from HUMSCs by forced expression of FOXA3, HNF1A, and HNF4A (collectively as 3TFs) and c-Myc. In vitro and in vivo functional experiments were conducted to demonstrate the hepatic phenotype, characterization, and function of HUMSC-derived HLCs (HUMSC-iHeps). ChIP-seq and RNA-seq were integrated to reveal the potential molecular mechanisms underlying c-Myc-mediated reprogramming.

Results

We showed that c-Myc greatly improved the trans-differentiation efficiency for HLCs from HUMSCs, which remained highly efficient in reprogramming fibroblasts into HLCs, suggesting c-Myc could promote direct reprogramming and its potentially widespread applicability for generating large amounts of HLCs in vitro. Mice transplantation experiments further confirmed the therapeutic potential of HUMSC-iHeps by liver function restoration and survival prolongation. Besides, in vivo safety assessment demonstrated the low risk of the tumorigenic potential of HUMSC-iHeps. We found that c-Myc functioned predominantly at an early phase of reprogramming, and we further unraveled the regulatory network altered by c-Myc.

Conclusions

c-Myc enhanced reprogramming efficiency of HLCs from HUMSCs. A large scale of functional HLCs generated more conveniently from HUMSCs could benefit biomedical studies and applications of liver diseases.
Systemic therapy of MSCs in bone regeneration: a systematic review and meta-analysis
Authors: Fu Jingfei , Wang Yanxue , Jiang Yiyang , Du Juan , Xu Junji , Liu Yi
Published: July 2021

Objectives

Over the past decades, many studies focused on mesenchymal stem cells (MSCs) therapy for bone regeneration. Due to the efficiency of topical application has been widely dicussed and systemic application was also a feasible way for new bone formation, the aim of this study was to systematically review systemic therapy of MSCs for bone regeneration in pre-clinical studies.

Methods

The article search was conducted in PubMed and Embase databases. Original research articles that assessed potential effect of systemic application of MSCs for bone regeneration in vivo were selected and evaluated in this review, according to eligibility criteria. The efficacy of MSC systemic treatment was analyzed by random effects meta-analysis, and the outcomes were expressed in standard mean difference (SMD) and its 95% confidence interval. Subgroup analyses were conducted on animal species and gender, MSCs types, frequency and time of injection, and bone diseases.

Results

Twenty-three articles were selected in this review, of which 21 were included in meta-analysis. The results showed that systemic therapy increased bone mineral density (SMD 3.02 [1.84, 4.20]), bone volume to tissue volume ratio (2.10 [1.16, 3.03]), and the percentage of new bone area (7.03 [2.10, 11.96]). Bone loss caused by systemic disease tended to produce a better response to systemic treatment (p=0.05 in BMD, p=0.03 in BV/TV).

Conclusion

This study concluded that systemic therapy of MSCs promotes bone regeneration in preclinical experiments. These results provided important information for the systemic application of MSCs as a potential application of bone formation in further animal experiments.
Mesenchymal stem cells alleviate idiopathic pneumonia syndrome by modulating T cell function through CCR2-CCL2 axis
Authors: Cao Min , Liu Huihui , Dong Yujun , Liu Wei , Yu Zhengyu , Wang Qingya , Wang Qingyun , Liang Zeying , Li Yuan , Ren Hanyun
Published: July 2021

Background

Idiopathic pneumonia syndrome (IPS) is a non-infectious fatal complication characterized by a massive infiltration of leukocytes in lungs and diffuse pulmonary injury after allogeneic hematopoietic stem cell transplantation (allo-HSCT). Conventional immunosuppressive treatments for IPS have poor therapeutic effects. Safe and effective treatments are not yet available and under explorations. Our previous study demonstrated that mesenchymal stem cells (MSCs) can alleviate IPS, but the mechanisms remain unclear.

Methods

Co-cultured pre-activated T cells and MSCs in vitro to observe the changes in the CCR2-CCL2 axis. By establishing an IPS mouse model and administering MSCs to further verify the results of in vitro experiments.

Results

Co-culture of pre-activated T cells with MSCs in vitro modulated the CCR2-CCL2 axis, resulting in quiescent T cells and polarization toward CCR2+CD4+ T cell subsets. Blocking CCR2-CCL2 interaction abolished the immunoregulatory effect of MSCs, leading to re-activation of T cells and partial reversion of polarizing toward CCR2+CD4+ T cells. In IPS mouse model, application of MSCs prolonged the survival and reduced the pathological damage and T cell infiltration into lung tissue. Activation of CCR2-CCL2 axis and production of CCR2+CD4+ T cells were observed in the lungs treated with MSCs. The prophylactic effect of MSCs on IPS was significantly attenuated by the administration of CCR2 or CCL2 antagonist in MSC-treated mice.

Conclusions

We demonstrated an important role of CCR2-CCL2 axis in modulating T cell function which is one of the mechanisms of the prophylactic effect of MSCs on IPS.
Embryonic stem cell-derived extracellular vesicles promote the recovery of kidney injury
Authors: Yu Lu , Liu Siying , Wang Chen , Zhang Chuanyu , Wen Yajie , Zhang Kaiyue , Chen Shang , Huang Haoyan , Liu Yue , Wu Lingling , Han Zhongchao , Chen Xiangmei , Li Zongjin , Liu Na
Published: July 2021

Background

Embryonic stem cell-derived extracellular vesicles (ESC-EVs) possess therapeutic potential for a variety of diseases and are considered as an alternative of ES cells. Acute kidney injury (AKI) is a common acute and severe disease in clinical practice, which seriously threatens human life and health. However, the roles and mechanisms of ESC-EVs on AKI remain unclear.

Methods

In this study, we evaluated the effects of ESC-EVs on physiological repair and pathological repair using murine ischemia-reperfusion injury-induced AKI model, the potential mechanisms of which were next investigated. EVs were isolated from ESCs and EVs derived from mouse fibroblasts as therapeutic controls. We then investigated whether ESC-EVs can restore the structure and function of the damaged kidney by promoting physiological repair and inhibiting the pathological repair process after AKI in vivo and in vitro.

Results

We found that ESC-EVs significantly promoted the recovery of the structure and function of the damaged kidney. ESC-EVs increased the proliferation of renal tubular epithelial cells, facilitated renal angiogenesis, inhibited the progression of renal fibrosis, and rescued DNA damage caused by ischemia and reperfusion after AKI. Finally, we found that ESC-EVs play a therapeutic effect by activating Sox9+ cells.

Conclusions

ESC-EVs significantly promote the physiological repair and inhibit the pathological repair after AKI, enabling restoration of the structure and function of the damaged kidney. This strategy might emerge as a novel therapeutic strategy for ESC clinical application.
Small blood stem cells for enhancing early osseointegration formation on dental implants: a human phase I safety study
Authors: Feng Sheng-Wei , Su Yi-Han , Lin Yen-Kuang , Wu Yu-Chih , Huang Yen-Hua , Yang Fu-Hung , Chiang Hsi-Jen , Yen Yun , Wang Peter Da-Yen
Published: July 2021

Background

Small blood stem cells (SB cells), isolated from human peripheral blood, demonstrated the ability to benefit bone regeneration and osseointegration. The primary goal of our study is to examine the safety and tolerability of SB cells in dental implantation for human patients with severe bone defects.

Methods

Nine patients were enrolled and divided into three groups with SB cell treatment doses of 1 × 105, 1 × 106, and 1 × 107SB cells, and then evaluated by computed tomography (CT) scans to assess bone mineral density (BMD) by Hounsfield units (HU) scoring. Testing was conducted before treatment and on weeks 4, 6, 8, and 12 post dental implantation. Blood and comprehensive chemistry panel testing were also performed.

Results

No severe adverse effects were observed for up to 6-month trial. Grade 1 leukocytosis, anemia, and elevated liver function were observed, but related with the patient’s condition or the implant treatment itself and not the transplantation of SB cells. The levels of cytokines and chemokines were detected by a multiplex immunological assay. Elevated levels of eotaxin, FGF2, MCP-1, MDC, and IL17a were found among patients who received SB cell treatment. This observation suggested SB cells triggered cytokines and chemokines for local tissue repair. To ensure the efficacy of SB cells in dental implantation, the BMD and maximum stresses via stress analysis model were measured through CT scanning. All patients who suffered from severe bone defect showed improvement from D3 level to D1 or D2 level. The HU score acceleration can be observed by week 2 after guided bone regeneration (GBR) and prior to dental implantation.

Conclusions

This phase I study shows that treatment of SB cells for dental implantation is well tolerated with no major adverse effects. The use of SB cells for accelerating the osseointegration in high-risk dental implant patients warrants further phase II studies.

Trial registration

Taiwan Clinical Trial Registry (SB-GBR001) and clinical trial registry of the United States (NCT04451486).
Sox9-expressing cells promote regeneration after radiation-induced lung injury via the PI3K/AKT pathway
Authors: Chen Shuang , Li Kang , Zhong Xinqi , Wang Ganping , Wang Xiaocheng , Cheng Maosheng , Chen Jie , Chen Zhi , Chen Jianwen , Zhang Caihua , Xiong Gan , Xu Xiuyun , Chen Demeng , Li Heping , Peng Liang
Published: July 2021

Background

Radiation-induced lung injury (RILI) is considered one of the most common complications of thoracic radiation. Recent studies have focused on stem cell properties to obtain ideal therapeutic effects, and Sox9 has been reported to be involved in stem cell induction and differentiation. However, whether Sox9-expressing cells play a role in radiation repair and regeneration remains unknown.

Methods

We successfully obtained Sox9CreER, RosatdTomato and RosaDTA mice and identified Sox9-expressing cells through lineage tracing. Then, we evaluated the effects of the ablation of Sox9-expressing cells in vivo. Furthermore, we investigated the underlying mechanism of Sox9-expressing cells during lung regeneration via an online single-cell RNA-seq dataset.

Results

In our study, we demonstrated that Sox9-expressing cells promote the regeneration of lung tissues and that ablation of Sox9-expressing cells leads to severe phenotypes after radiation damage. In addition, analysis of an online scRNA-Seq dataset revealed that the PI3K/AKT pathway is enriched in Sox9-expressing cells during lung epithelium regeneration. Finally, the AKT inhibitor perifosine suppressed the regenerative effects of Sox9-expressing cells and the AKT pathway agonist promotes proliferation and differentiation.

Conclusions

Taken together, the findings of our study suggest that Sox9-expressing cells may serve as a therapeutic target in lung tissue after RILI.
Gender disparity in critical care publications: a novel Female First Author Index
Authors: Chary Sowmya , Amrein Karin , Soeteman Djøra I. , Mehta Sangeeta , Christopher Kenneth B.
Published: July 2021

Background

Bibliometric analyses show gender bias against women in scientific publications and citations. We hypothesized that a metric of an individual senior author’s inclusivity of women as first authors in critical care publications would predict gender inequality.

Methods

Using PubMed and Web of Science, we conducted a bibliometric analysis of original research publications in critical care from 2008 to 2018 in 11 specialty and general journals. Gender for first and senior authors was assigned by a gender determination application, and manually if needed. For all senior authors we defined the novel Female First Author Index (FFA-index) = #Female first authors in publications by an individual senior author/Total # publications by that senior author. We produced a novel interactive web-based application using the R package Shiny to increase potential utilization of the FFA-index.

Results

Of 7370 publications, 30.4% had female first authors and 15.5% had female senior authors. After adjustment for impact factor, journal, year of publication, number of authors, country, and gender determination accuracy, female senior authorship was associated with a 1.9-fold increase in female first authorship [OR = 1.85 (95% CI 1.62, 2.11); p < 0.001] compared with male senior authorship. The mean (SD) FFA-index for all individual senior authors was 30.5 (42.9); with a significant difference in FFA-index between male and female senior authors (27.6 versus 42.5, respectively; p < 0.001). The interactive web-based application (FFA-index App) produces the same FFA-index output as our study results.

Conclusions

Female representation at prominent authorship positions in critical care publications is still far from achieving gender parity. By creating an authorship index score, we propose a frame of reference for the advancement of female first authorship.
Long-term quality of life in necrotizing soft-tissue infection survivors: a monocentric prospective cohort study
Authors: Urbina Tomas , Canoui-Poitrine Florence , Hua Camille , Layese Richard , Alves Aline , Ouedraogo Rachida , Bosc Romain , Sbidian Emilie , Chosidow Olivier , Dessap Armand Mekontso , de Prost Nicolas ,   Henri Mondor Hospital Necrotizing Fasciitis Group, Bosc Romain , Chosidow Olivier , de Prost Nicolas , Hua Camille , Lepeule Raphaël , Luciani Alain , Nakad Lionel , Tomberli Françoise , Urbina Tomas , Woerther Paul-Louis
Published: July 2021

Background

Compared to other life-threatening infection survivors, long-term health-related quality of life (QOL) of patients surviving necrotizing soft-tissue infections (NSTI) and its determinants are little known.

In this monocentric prospective cohort including NSTI survivors admitted between 2014 and 2017, QOL was assessed during a phone interview using the 36-Item Short-Form Health Survey (SF-36), the Hospital Anxiety and Depression (HAD), the activity of daily living (ADL), instrumental ADL (IADL) scales and the Impact of Event Scale-Revised (IES-R). The primary outcome measure was the SF-36 physical component summary (PCS). NSTI patients were compared according to intensive care unit (ICU) admission status. ICU survivors were matched on SAPS II with non-NSTI related septic shock survivors.

Results

Forty-nine NSTI survivors were phone-interviewed and included in the study. Median PCS was decreased compared to the reference population [− 0.97 (− 2.27; − 0.08) SD]. Previous cardiac disease was the only variable associated with PCS alteration [multivariate regression coefficient: − 8.86 (− 17.64; − 0.07), p  =  0.048]. Of NSTI survivors, 15.2% had a HAD-D score  ≥  5 and 61.2% an IES-R score  ≥  33. ICU admission was not associated with lower PCS [35.21 (25.49–46.54) versus (vs) 41.82 (24.12–51.01), p  =  0.516], but with higher IES-R score [14 (7.5–34) vs 7 (3–18), p  =  0.035] and a higher proportion of HAD-D score  ≥  5 (28.6 vs 4.0%, p  =  0.036). Compared to non-NSTI septic shock-matched controls, NSTI patients had similar PCS [33.81 (24.58; − 44.39) vs 44.87 (26.71; − 56.01), p  =  0.706] but higher HAD-D [3.5 (1–7) vs 3 (1.5–6), p  =  0.048] and IES-R scores [18 (8–35) vs 8 (3–19), p  =  0.049].

Conclusions

Long-term QOL in NSTI survivors is severely impaired, similarly to that of non-NSTI septic shock patients for physical compartments, but with more frequent depressive and/or post-traumatic stress disorders. Only ICU admission and previous cardiac disease were predictive of QOL impairment.
Racial and ethnic disparities in viral acute respiratory infections in the United States: protocol of a systematic review
Authors: Prata Menezes Neia , Malone Jowanna , Lyons Carrie , Cadet Kechna , Dean Lorraine , Millett Gregorio , Baral Stefan
Published: July 2021

Background

The COVID-19 pandemic caused by SARS-CoV-2 has highlighted consistent inequities in incidence, disease severity, and mortality across racial and ethnic minority populations in the United States (US) and beyond. While similar patterns have been observed with previous viral respiratory pathogens, to date, no systematic review has comprehensively documented these disparities or potential contributing factors. In response, this review aims to synthesize data on racial and ethnic disparities in morbidity and mortality due to viral acute respiratory infections (ARI) other than SARS-CoV-2. This review will focus on understanding structural health and social factors to contextualize race and ethnicity driving these disparities in the US.

Methods

We will conduct a systematic review of studies published from January 1, 2002, onward. Our search will include PubMed/MEDLINE, EBSCO Host-CINAHL Plus, PsycInfo, EMBASE, and Cochrane Library databases to identify relevant articles. We will include studies of any design that describe racial/ethnic disparities associated with viral ARI conducted in the US. Primary outcomes include incidence, disease severity or complication, hospitalization, or death attributed to ARI. Secondary outcomes include uptake of preventive interventions including vaccination, handwashing, social distancing, and wearing masks. Two reviewers will independently screen all citations, full-text articles, and abstract relevant data. Data characterizing individual-, community-, and structural-level factors associated with these disparities will be abstracted to better understand the underlying structural inequities contributing to racial disparities in ARI. We will assess the methodological quality of all studies and will conduct meta-analyses using random effects models if appropriate.

Discussion

Findings from this systematic review will shed light on patterns of racial and ethnic disparities in viral ARI in the United States to support mathematical modeling of epidemic trajectories, intervention impact, and structural drivers of transmission, including structural racism. Moreover, data emerging from this review may reignite pandemic preparedness focused on communities with specific vulnerabilities related to living and working conditions given prevailing structural inequities, thus facilitating improved future pandemic responses to novel or endemic viral respiratory pathogens.

Systematic review registration

PROSPERO CRD42020219771
AI business model: an integrative business approach
Authors: Mishra Shrutika , Tripathi A. R.
Published: July 2021

Artificial intelligence is the ecosphere’s prevalent and most comprehensive general acquaintance common-sense cognitive engine. The artificial intelligence (AI) business platform model is virtually at affluence with cloud SaaS model. It concerns AI solutions that can work together on the top layer of the other digital systems, like a Customer Relationship Management (CRM) and Enterprise Resource Planning (ERP) business system. AI admittances in the digital data fluid through the coordination, fueling business enhancements over phases. In this business model, the business will safekeep a recurrent subscription. This paper endeavors to emphasize on the preventative side of the use of AI and machine learning (ML) technology to enterprise digital platform business model innovation and business dynamics. We acme the strategic implications and innovations with analytics. We explore the derivations of data-driven insights, models, and visualizations.
COVID-19 and eating disorder and mental health concerns in patients with eating disorders
Authors: Vitagliano Julia A. , Jhe Grace , Milliren Carly E. , Lin Jessica A. , Spigel Rebecca , Freizinger Melissa , Woods Elizabeth R. , Forman Sara F. , Richmond Tracy K.
Published: July 2021

Background

The Coronavirus (COVID-19) pandemic dramatically transformed daily life for adolescents and young adults, altering social and physical environments. Previous research has shown such shifts in daily life to be especially challenging for people living with eating disorders (ED). However, the extent of this environmental change on ED symptoms and mental health (MH) has been relatively unexplored in patients with EDs. This study examines how young people with EDs feel the COVID-19 pandemic has affected their living environments as well as their ED and MH symptoms and motivation for ED recovery.

Methods

Participants were enrollees in the Registry of Eating Disorders and their Co-morbidities OVER time in Youth (RECOVERY) who responded to an additional survey (n = 89) in July 2020 to assess their perceptions of the impact of the COVID-19 pandemic. Participants reported on concerns of their ED worsening due to increased time living in a “triggering environment” due to the pandemic as well as perceived COVID-related changes in intrusive ED thoughts, depression, anxiety, isolation, and motivation to recover. Logistic regression models, adjusted for age and ED diagnosis, examined the association of triggering environment with ED and MH symptoms.

Results

The majority of respondents reported concern for worsening of their ED due to a “triggering environment” (63%). Most reported an increase in ED thoughts (74%), feelings of anxiety (77%), depression (73%), and isolation (80%) they perceived to be related to the pandemic. Nearly one-third reported decrease in motivation to recover (29%) they perceived to be related to the pandemic. After adjusting for age and ED diagnosis, participants who reported concern for worsening of their ED due to a triggering environment had nearly 18 times the odds of decreased motivation to recover (OR 18.1; 95% CI 3.37–97.4, p = 0.003) and nearly 24 times the odds of increased ED thoughts (OR 23.8; 95% CI 4.31–131.6, p < 0.001) compared to those who did not report concern for worsening of their ED due to a triggering environment.

Conclusions

Our findings demonstrate the perceived negative impact the COVID-19 pandemic has had on the self-reported ED and MH symptoms in patients with EDs, particularly in those who report concern for a negative environmental change. These results underscore the need for heightened monitoring of patients with EDs during the pandemic.
Imaging biomarkers for evaluating tumor response: RECIST and beyond
Authors: Ko Ching-Chung , Yeh Lee-Ren , Kuo Yu-Ting , Chen Jeon-Hor
Published: July 2021

Response Evaluation Criteria in Solid Tumors (RECIST) is the gold standard for assessment of treatment response in solid tumors. Morphologic change of tumor size evaluated by RECIST is often correlated with survival length and has been considered as a surrogate endpoint of therapeutic efficacy. However, the detection of morphologic change alone may not be sufficient for assessing response to new anti-cancer medication in all solid tumors. During the past fifteen years, several molecular-targeted therapies and immunotherapies have emerged in cancer treatment which work by disrupting signaling pathways and inhibited cell growth. Tumor necrosis or lack of tumor progression is associated with a good therapeutic response even in the absence of tumor shrinkage. Therefore, the use of unmodified RECIST criteria to estimate morphological changes of tumor alone may not be sufficient to estimate tumor response for these new anti-cancer drugs. Several studies have reported the low reliability of RECIST in evaluating treatment response in different tumors such as hepatocellular carcinoma, lung cancer, prostate cancer, brain glioma, bone metastasis, and lymphoma. There is an increased need for new medical imaging biomarkers, considering the changes in tumor viability, metabolic activity, and attenuation, which are related to early tumor response. Promising imaging techniques, beyond RECIST, include dynamic contrast-enhanced computed tomography (CT) or magnetic resonance imaging (MRI), diffusion-weight imaging (DWI), magnetic resonance spectroscopy (MRS), and18 F-fluorodeoxyglucose (FDG) positron emission tomography (PET). This review outlines the current RECIST with their limitations and the new emerging concepts of imaging biomarkers in oncology.
Domain randomization for neural network classification
Authors: Valtchev Svetozar Zarko , Wu Jianhong
Published: July 2021

Large data requirements are often the main hurdle in training neural networks. Convolutional neural network (CNN) classifiers in particular require tens of thousands of pre-labeled images per category to approach human-level accuracy, while often failing to generalized to out-of-domain test sets. The acquisition and labelling of such datasets is often an expensive, time consuming and tedious task in practice. Synthetic data provides a cheap and efficient solution to assemble such large datasets. Using domain randomization (DR), we show that a sufficiently well generated synthetic image dataset can be used to train a neural network classifier that rivals state-of-the-art models trained on real datasets, achieving accuracy levels as high as 88% on a baseline cats vs dogs classification task. We show that the most important domain randomization parameter is a large variety of subjects, while secondary parameters such as lighting and textures are found to be less significant to the model accuracy. Our results also provide evidence to suggest that models trained on domain randomized images transfer to new domains better than those trained on real photos. Model performance appears to remain stable as the number of categories increases.
Probing mechanobiological role of filamin A in migration and invasion of human U87 glioblastoma cells using submicron soft pillars
Authors: Ketebo Abdurazak Aman , Park Chanyong , Kim Jaewon , Jun Myeongjun , Park Sungsu
Published: July 2021

Filamin A (FLNa) belongs to an actin-binding protein family in binding and cross-linking actin filaments into a three-dimensional structure. However, little attention has been given to its mechanobiological role in cancer cells. Here, we quantitatively investigated the role of FLNa by analyzing the following parameters in negative control (NC) and FLNa-knockdown (KD) U87 glioma cells using submicron pillars (900 nm diameter and 2 μm height): traction force (TF), rigidity sensing ability, cell aspect ratio, migration speed, and invasiveness. During the initial phase of cell adhesion (< 1 h), FLNa-KD cells polarized more slowly than did NC cells, which can be explained by the loss of rigidity sensing in FLNa-KD cells. The higher motility of FLNa-KD cells relative to NC cells can be explained by the high TF exerted by FLNa-KD cells when compared to NC cells, while the higher invasiveness of FLNa-KD cells relative to NC cells can be explained by a greater number of filopodia in FLNa-KD cells than in NC cells. Our results suggest that FLNa plays important roles in suppressing motility and invasiveness of U87 cells.
Terrestrial planet compositions controlled by accretion disk magnetic field
Authors: McDonough William F. , Yoshizaki Takashi
Published: July 2021

Terrestrial planets (Mercury, Venus, Earth, and Mars) are differentiated into three layers: a metallic core, a silicate shell (mantle and crust), and a volatile envelope of gases, ices, and, for the Earth, liquid water. Each layer has different dominant elements (e.g., increasing iron content with depth and increasing oxygen content to the surface). Chondrites, the building blocks of the terrestrial planets, have mass and atomic proportions of oxygen, iron, magnesium, and silicon totaling ≥ 90% and variable Mg/Si (∼ 25%), Fe/Si (factor of ≥2), and Fe/O (factor of ≥ 3). What remains an unknown is to what degree did physical processes during nebular disk accretion versus those during post-nebular disk accretion (e.g., impact erosion) influence these planets final bulk compositions. Here we predict terrestrial planet compositions and show that their core mass fractions and uncompressed densities correlate with their heliocentric distance, and follow a simple model of the magnetic field strength in the protoplanetary disk. Our model assesses the distribution of iron in terms of increasing oxidation state, aerodynamics, and a decreasing magnetic field strength outward from the Sun, leading to decreasing core size of the terrestrial planets with radial distance. This distribution enhances habitability in our solar system and may be equally applicable to exoplanetary systems.
Investigation of distinctive physico-chemical soil correlations for Kirkuk city using spatial analysis technique incorporated with statistical modeling
Authors: Raheem Aram Mohammed , Omar Najat Qader
Published: July 2021

In the sense of challenging economic situation, it is difficult to perform laboratory tests in a whole intended area to define the soil characteristics for whatever task or situation within an entire city. Hence, this study has used the spatial analysis through applying the inverse distance weighted (IDW) technique to integrate with the available data for 56 different soil samples collected from various depths of Kirkuk city. The studied characteristics were mainly classified to physical soil properties included gravel, sand, silt, and clay contents with chemical soil properties included organic and gypsum contents, pH content, sulfur trioxide, and total dissolved solids (TDS) content. Moreover, statistical investigations such as physico-chemical correlation, linear single and linear multi regression models have been applied. The results of this study have focused on constructing thematic digital maps for visualizing different physical and chemical characteristics of Kirkuk soil. In statistical analysis, negative linear correlations are obtained from the relation between the values of the pH, gypsum, organic, and SO3 contents with the increase of the gravel content, whereas a positive linear correlation is attained for the values of TDS content with the increase of the gravel content. Furthermore, the proposed linear multi regression model predicts the pH values reasonably where most of the values were close to the equality line. The obtained digital maps accompanied with the statistical models will provide comprehensive spatial, agricultural, chemical and mechanical visualizations of the nature and morphology of the soils in Kirkuk city.
Nutritional quality of beverages available in vending machines in health and social care institutions: do we really want such offers?
Authors: Rozman Urška , Mis Nataša Fidler , Kupirovič Urška Pivk , Pravst Igor , Kocbek Primož , Strauss Maja , Turk Sonja Šostar
Published: July 2021

Background

Vending machines represent one way of offering food, but they are overlooked in the efforts to improve people’s eating habits. The aim of our study was to analyse the variety and nutritional values of beverages offered in vending machines in social and health care institution in Slovenia.

Methods

The available beverages were quantitatively assessed using traffic light profiling and the model for nutrient profiling used by Food Standards Australia New Zealand. Vending machines in 188 institutions were surveyed, resulting in 3046 different beverages consisting of 162 unique product labels.

Results

Between 51 and 54% of beverages were categorised as unhealthy with regard to sugar content. Water accounted for only 13.7% of all beverages in vending machines. About 82% of beverages in vending machines were devoted to sugar-sweetened beverages, the majority (58.9%) presented in 500-ml bottles. The average sugar content and average calories in beverages sold in vending machines are slightly lower than in beverages sold in food stores.

Conclusions

We suggest that regulatory guidelines should be included in the tender conditions for vending machines in health and social care institutions, to ensure healthy food and beverage choices.