bluuebunny/crossref_metadata_2025 huggingface dataset:
Randomized, Double-Blind, Controlled Study to Evaluate the Effect of Vidian Nerve Cauterization on Lacrimation
Authors: Su Wan-Fu , Liu Shao-Cheng , Hsu Wei-Cherng , Chen Yu-Cheng
Published: May 2014

Background

After vidian neurectomy, low reported rates of dry eye syndrome (DES) seemed incompatible with the high success rate of nerve severance in previous studies. This study aimed at understanding of the pathophysiology of lacrimation and evaluating the effect of thermal injury through the distal stump on the sphenopalatine ganglion (SPG) after vidian neurectomy.

Methods

A randomized, double-blind, controlled study was performed to evaluate the DES. Eighty precise vidian neurectomies were randomized in a 1:1 ratio to groups 1 and 2. Group 1 represented the cauterization and was used in both distal and proximal nerve stumps, whereas only the proximal nerve stump was cauterized in group 2 subjects. The DES was evaluated with Schirmer's test and ocular surface disease index (OSDI) before and after surgery at 7–10 days and 30 days, respectively.

Results

In group 1, the Schirmer's test showed a mean decline of 20 mm (20/30, 66%) at 7–10 days and 15 mm (15/30, 50%) at 30 days. In group 2, the Schirmer's test revealed significantly lesser dry eye problems, with a mean decline of 16 mm (16/30; 52%) at 7–10 days and 2 mm (2/30; 6%) at 30 days. The significantly less postoperative dry eye problems in group 2 can be shown by the OSDI at 7–10 days, but not at 30 days. The mean follow-up period was 24 months. No recurrence of nasal allergy symptoms was noted in the follow up period.

Conclusion

The significant advantage of preservation of the SPG function is justified by Schirmer's test, although the effect did not appear to be comparable with the clinical manifestations evaluated by OSDI at 30 days. Nevertheless, the preservation of distal stump from preventive cauterization can still offer better eye ball moisture in the early evaluation of DES.
Aspirin Hypersensitivity in Patients with Chronic Rhinosinusitis and Nasal Polyposis: Frequency and Contributing Factors
Authors: Nabavi Mohammad , Esmaeilzadeh Hossein , Arshi Saba , Bemanian Mohammad Hassan , Fallahpour Morteza , Bahrami Ahmad , Mortazavi Negar , Kamrava Kamran , Farhadi Mohammad , Taghipour Reza , Rezaei Nima
Published: May 2014

Background

Aspirin-exacerbated respiratory disease (AERD) is a syndrome frequently seen in patients with chronic rhinosinusitis and nasal polyposis (CRSwNP). However, there are few studies on evaluating the prevalence of aspirin (acetylsalicylic acid [ASA]) hypersensitivity in patients with CRSwNP using the oral aspirin challenge (OAC) test. This cross-sectional study was designed to determine the frequency of ASA hypersensitivity and factors associated with it in patients with CRSwNP in Tehran, Iran.

Methods

Adult patients with CRSwNP who were presented to the asthma and allergy clinic were recruited for the study. After confirming CRS and NP, OAC was performed to evaluate/confirm the diagnosis of ASA hypersensitivity. Atopic evaluation was performed using skin-prick test, nasal smear, blood eosinophil count, and serum total IgE.

Results

Eighty Iranian patients (43 women and 37 men) with CRSwNP were enrolled (mean age, 38.9 ± 10.7 years). OAC was performed in all of the patients and 39 patients (48.8%) had a positive reaction; among them, 14 (35.8%) had a self-reported history of ASA hypersensitivity. Concomitant asthma, previous polyp surgery, high polyp score, and ASA hypersensitivity history were all associated with positive OAC (p < 0.05). Presence of AERD was not associated with age, stage of asthma, blood eosinophilia, nasal smear eosinophilia, and atopy.

Conclusion

ASA hypersensitivity is common in patients with CRSwNP in Tehran, Iran. Patients at risk for AERD should be evaluated for the presence of ASA hypersensitivity with ASA provocation challenge test to confirm the diagnosis.
In the (Sub)Tropics Allergic Rhinitis and Its Impact on Asthma Classification of Allergic Rhinitis is More Useful than Perennial–Seasonal Classification
Authors: Larenas-Linnemann Désirée , Michels Alexandra , Dinger Hanna , Arias-Cruz Alfredo , Moreno Marichuy Ambriz , Barajas Martin Bedolla , Javier Ruth Cerino , del Prado Maria de la Luz Cid , Moreno Manuel Alejandro Cruz , Vergara Laura Diego , Almaráz Roberto García , García-Cobas Cecilia Y. , Imperial Daniel Alberto Garcia , Garcia Muñoz Rosa , Colín Dante Hernandez , Zapien Francisco Javier Linares , Agustín Luna Pech Jorge , Campos Juan Jose Matta , Jimenez Norma Martinez , Avalos Miguel Medina , Hernandez Alejandra Medina , Maldonado Albero Monteverde , López Doris Nereida , Nazara Luis Julian Pizano , Sanchez Emanuel Ramirez , López José Domingo Ramos , Rodriguez-Pérez Noel , Rodriguez Ortiz Pablo G. , Shah-Hosseini Kijawasch , Mösges Ralph
Published: May 2014

Background

Two different allergic rhinitis (AR) symptom phenotype classifications exist. Treatment recommendations are based on intermittent–persistent (INT-PER) cataloging, but clinical trials still use the former seasonal AR–perennial AR (SAR-PAR) classification. This study was designed to describe how INT-PER, mild–moderate/severe and SAR-PAR of patients seen by allergists are distributed over the different climate zones in a (subtropical country and how these phenotypes relate to allergen sensitization patterns.

Methods

Six climate zones throughout Mexico were determined, based on National Geographic Institute (Instituto Nacional de Estadística y Geografía) data. Subsequent AR patients (2–68 years old) underwent a blinded, standardized skin-prick test and filled out a validated questionnaire phenotyping AR.

Results

Five hundred twenty-nine subjects participated in this study. In the tropical zone with 87% house-dust mite sensitization, INT (80.9%; p < 0.001) and PAR (91%; p = 0.04) were more frequent than in the subtropics. In the central high-pollen areas, there was less moderate/severe AR (65.5%; p < 0.005). Frequency of comorbid asthma showed a clear no -south gradient, from 25% in the dry north to 59% in the tropics (p < 0.005). No differences exist in AR cataloging among patients with different sensitization patterns, with two minor exceptions (more PER in tree sensitized and more PAR in mold positives; p < 0.05).

Conclusion

In a (sub)tropical country the SAR-PAR classification seems of limited value and bears poor relation with the INT-PER classification. INT is more frequent in the tropical zone. Because PER has been shown to relate to AR severity, clinical trials should select patients based on INT-PER combined with the severity cataloging because these make for a better treatment guide than SAR-PAR.
Chronic Cholesterol Depletion by Lovastatin Suppresses MUC5AC Gene Expression in Human Airway Epithelial Cells
Authors: Lee Eun Jung , Song Kee Jae , Kwon Jin Ho , Park Ah Young , Jo Kwang-Hee , Kim Kyung-Su
Published: May 2014

Background

We recently showed that acute cholesterol depletion in the plasma membrane of NCI-H292 cells by methyl-β-cyclodextrin suppressed IL-1beta–induced MUC5AC gene expression. Because cholesterol depletion is clinically used as an antihypersecretory method, chronic cholesterol depletion by lovastatin is more rational and safe than acute depletion. Therefore, we sought to investigate whether chronic cholesterol depletion by lovastatin is feasible and, if so, suppresses the expression of GMUC5AC in NCI-H292 cells. We also considered whether this alteration of MUC5AC expression is related to IL-1 receptor and mitogen-activated protein kinase (MAPK) activity.

Methods

After NCI-H292 cells were pretreated with 10 μM of lovastatin for 1 hour, 10 ng/mL of IL-1β was added and cotreated with lovastatin for 24 hours. MUC5AC mRNA expression was then determined by real-time polymerase chain reaction. Cholesterol depletion by lovastatin was measured by modified microenzymatic fluorescence assay and filipin staining. The phosphorylation of IL-1 receptor, ERK, and p38 MAPK as analyzed by Western blot.

Results

Cholesterol in the plasma membrane was significantly depleted by lovastatin treatment for 24 hours. IL-1beta0-induced MUC5AC mRNA expression was decreased by lovastatin and this decrease occurred IL-1 receptor specifically. Lovastatin suppressed the activation of p38 MAPK but not ERK1/2 in cells activated with IL-1beta. This result suggests that lovastatin-mediated suppression L-1beta–induced MUC5AC mRNA operated only viathe p38 MAPK-dependent pathway.

Conclusion

Chronic cholesterol depletion in the plasma membrane of NCI-H292 cells may be considered an antihypersecretory method, because it effectively inhibits mucin gene expression of human airway epithelial cells.
Intranasal Landmarks for Adequate L-Strut Preservation during Endoscopic Septoplasty
Authors: Seth Rahul , Haffey Timothy , McBride Jennifer M. , Sindwani Raj
Published: May 2014

Background

A 10- to 15-mm L-strut in the dorsal and caudal areas of the nasal septum is preserved during septoplasty to maintain structural stability and prevent external deformity. However, during endoscopically performed septoplasty the surgeon has a very different perspective, which may create a more challenging situation to ensure adequate strut preservation. No reliable intranasal landmarks have been established. We establish intranasal landmarks that may be used during endoscopic septoplasty (ES) to safely maintain sufficient caudal and dorsal support. A cadaveric anatomic study was performed.

Methods

Measurements were made using calipers in 15 human cadavers (8 male and 7 female subjects), totaling 30 nasal passages. The caudal strut distance was measured from the inferior concha to the caudal aspect of the quadrangular cartilage. The dorsal strut height was measured from a line parallel to the nasal dorsum that extended from the middle turbinate (MT) axilla to the nasal vestibule (termed the “axillary line”).

Results

Using the inferior concha and the vertical MT attachment as endoscopic landmarks, the average caudal and dorsal struts measured 18.9 and 16.4 mm, respectively. Strut size was larger in male subjects than female subjects; however, dimensions remained >10 mm in all cadavers, establishing these landmarks as safe and effective for determining extent of cartilage resection.

Conclusion

The inferior turbinate and vertical MT attachment may be used to guide extent of cartilage resection during ES. Use of the described conchal and axillary lines as landmarks allow for preservation of an adequate L-strut during ES and may reduce support related complications.
Azelastine Desensitization of Transient Receptor Potential Vanilloid 1: A Potential Mechanism Explaining its Therapeutic Effect in Nonallergic Rhinitis
Authors: Singh Umesh , Bernstein Jonathan A. , Haar Lauren , Luther Kristin , Jones Walter K.
Published: May 2014

Background

Capsaicin, a prototypic transient receptor potential vanilloid 1 (TRPV1) agonist, has been shown to be more clinically effective in the treatment of nonallergic rhinitis (NAR) compared with other rhinitis subtypes. Azelastine has also been found to be clinically effective in the treatment of NAR but its mechanism(s) of action is still poorly elucidated. This study was designed to determine, using in vitro cell lines, whether topical therapies including azelastine have activity on TRPV1 ion channels similar to capsaicin.

Methods

The effects of capsaicin (1 μM), azelastine (30 μM), bepotastine (10 μM), olopatadine (10 μM), and fluticasone (200 μM) on TRPV1 channels using mice neuronal cells (Cath.a), as surrogates for submucosal sensory neurons, and human nasal epithelial cells (hNEC) were determined and compared. For azelastine, bepotastine, and capsaicin, which elicited an agonist effect on TRPV1, live cell [Ca2+] signaling in Cath.a cells and hNECs expressing TRPV1 were performed in the absence and presence of capsazepine at 10 μM (a TRPV1 antagonist) using wild-type mouse embryonic fibroblasts (wtMEFs) that express TRPV1 ion channels and TRPV1 homozygous null mutant (TRPV1 -/-) knockout MEF cells as controls to establish TRPV1 channel selectivity. As azelastine has previously been found clinically effective in NAR, additional experiments were performed to determine its ability to desensitize TRPV1 ion channels and its effect on regulating intracellular calcium homeostasis.

Results

Cath.a cells treated with azelastine, bepotastine, or capsaicin showed a significant increase in TRPV1-dependant (Ca2+) specific cytosolic fluorescence. Continuous treatment with azelastine or capsaicin resulted in desensitization of TRPV1 channels. In hNECs, azelastine stimulation resulted in Ca2+ shifts from the cytosol to mitochondria and overexpression of hematopoietic cell-specific Lyn substrate 1–associated protein X1, which may thus be effective in cytosolic Ca2+ homeostasis.

Conclusion

Azelastine, similar to capsaicin, exhibits direct activity on TRPV1 ion channels that may represent a novel mechanistic pathway explaining its clinical efficacy in NAR.
Biofuel derived from enzymatic degummed linum usitatissimum seed oil : as a potential resource for diesel engine
Authors: Kanakraj S , Dixit S , Rehman A
Published: May 2014

A bio-fuel derived from enzymatic degummed Linum usitatissimum seed oil (EDLO). We applied response surface methodology in order to optimize the reaction conditions of degumming process using microbial enzyme (Licitase ultra) in Linum usitatissimum seed oil, Enzyme dosage, temperature and reaction time were important determining factors affecting oil degumming. Optimum operating conditions were found to be reaction time of 5 h, enzyme dosage of 1.6 wt% and temperature of 50°C at pH 5. At this optimum condition point the phosphorus content of degummed oil were found to be 7.8 mg/kg. This study also discusses the EDLO-diesel fuel blends as a potential source for blend production. Furthermore, the blends of various proportions of EDLO:diesel,25:75, 35:65, 45:55 and 55: 45 were prepared, characterized and compared with diesel fuel. The fuel properties of EDLO25 diesel fuel blend was found comparable to those of diesel on the basis of experimental analysis.. DOI: http://dx.doi.org/10.3329/bjsir.v49i1.18849 Bangladesh J. Sci. Ind. Res. 49(1), 13-24, 2014
Mcl-1 Ubiquitination: Unique Regulation of an Essential Survival Protein
Authors: Mojsa Barbara , Lassot Iréna , Desagher Solange
Published: May 2014

Mcl-1 is an anti-apoptotic protein of the Bcl-2 family that is essential for the survival of multiple cell lineages and that is highly amplified in human cancer. Under physiological conditions, Mcl-1 expression is tightly regulated at multiple levels, involving transcriptional, post-transcriptional and post-translational processes. Ubiquitination of Mcl-1, that targets it for proteasomal degradation, allows for rapid elimination of the protein and triggering of cell death, in response to various cellular events. In the last decade, a number of studies have elucidated different pathways controlling Mcl-1 ubiquitination and degradation. Four different E3 ubiquitin-ligases (e.g., Mule, SCFβ-TrCP, SCFFbw7 and Trim17) and one deubiquitinase (e.g., USP9X), that respectively mediate and oppose Mcl-1 ubiquitination, have been formerly identified. The interaction between Mule and Mcl-1 can be modulated by other Bcl-2 family proteins, while recognition of Mcl-1 by the other E3 ubiquitin-ligases and deubiquitinase is influenced by phosphorylation of specific residues in Mcl-1. The protein kinases and E3 ubiquitin-ligases that are involved in the regulation of Mcl-1 stability vary depending on the cellular context, highlighting the complexity and pivotal role of Mcl-1 regulation. In this review, we attempt to recapitulate progress in understanding Mcl-1 regulation by the ubiquitin-proteasome system.
Development of a Recombinase Polymerase Amplification Assay for the Detection of Pathogenic Leptospira
Authors: Ahmed Ahmed , Van der Linden Hans , Hartskeerl Rudy
Published: May 2014

Detection of leptospires based on DNA amplification techniques is essential for the early diagnosis of leptospirosis when anti-Leptospira antibodies are below the detection limit of most serological tests. In middle and low income countries where leptospirosis is endemic, routine implementation of real-time PCR is financially and technically challenging due to the requirement of expensive thermocycler equipment. In this study we report the development and evaluation of a novel isothermal recombinase polymerase amplification assay (RPA) for detection of pathogenic Leptospira based on TwistAmp chemistry. RPA enabled the detection of less than two genome copies per reaction. Retrospective evaluation revealed a high diagnostic accuracy (sensitivity and specificity of 94.7% and 97.7%, respectively) compared to culturing as the reference standard. RPA presents a powerful tool for the early diagnosis of leptospirosis in humans and in animals. Furthermore, it enables the detection of the causative agent in reservoirs and environment, and as such is a valuable adjunct to current tools for surveillance and early outbreak warning.
Mental and Behavioral Disorders Due to Substance Abuse and Perinatal Outcomes: A Study Based on Linked Population Data in New South Wales, Australia
Authors: Bonello Michelle , Xu Fenglian , Li Zhuoyang , Burns Lucy , Austin Marie-Paule , Sullivan Elizabeth
Published: May 2014

Background: The effects of mental and behavioral disorders (MBD) due to substance use during peri-conception and pregnancy on perinatal outcomes are unclear. The adverse perinatal outcomes of primiparous mothers admitted to hospital with MBD due to substance use before and/or during pregnancy were investigated. Method: This study linked birth and hospital records in NSW, Australia. Subjects included primiparous mothers admitted to hospital for MBD due to use of alcohol, opioids or cannabinoids during peri-conception and pregnancy. Results: There were 304 primiparous mothers admitted to hospital for MBD due to alcohol use (MBDA), 306 for MBD due to opioids use (MBDO) and 497 for MBD due to cannabinoids (MBDC) between the 12 months peri-conception and the end of pregnancy. Primiparous mothers admitted to hospital for MBDA during pregnancy or during both peri-conception and pregnancy were significantly more likely to give birth to a baby of low birthweight (AOR = 4.03, 95%CI: 1.97–8.24 for pregnancy; AOR = 9.21, 95%CI: 3.76–22.57 both periods); preterm birth (AOR = 3.26, 95% CI: 1.52–6.97 for pregnancy; AOR = 4.06, 95%CI: 1.50–11.01 both periods) and admission to SCN or NICU (AOR = 2.42, 95%CI: 1.31–4.49 for pregnancy; AOR = 4.03, 95%CI: 1.72–9.44 both periods). Primiparous mothers admitted to hospital for MBDO, MBDC or a combined diagnosis were almost three times as likely to give birth to preterm babies compared to mothers without hospital admissions for psychiatric or substance use disorders. Babies whose mothers were admitted to hospital with MBDO before and/or during pregnancy were six times more likely to be admitted to SCN or NICU (AOR = 6.29, 95%CI: 4.62–8.57). Conclusion: Consumption of alcohol, opioids or cannabinoids during peri-conception or pregnancy significantly increased the risk of adverse perinatal outcomes.
Human Papillomavirus 16 E6 Contributes HIF-1α Induced Warburg Effect by Attenuating the VHL-HIF-1α Interaction
Authors: Guo Yi , Meng Xiangkai , Ma Jiaming , Zheng Yahong , Wang Qian , Wang Yanan , Shang Hong
Published: May 2014

Cervical cancer is still one of the leading causes of cancer deaths in women worldwide, especially in the developing countries. It is a major metabolic character of cancer cells to consume large quantities of glucose and derive more energy by glycolysis even in the presence of adequate oxygen, which is called Warburg effect that can be exaggerated by hypoxia. The high risk subtype HPV16 early oncoprotein E6 contributes host cell immortalization and transformation through interacting with a number of cellular factors. Hypoxia-inducible factor 1α (HIF-1α), a ubiquitously expressed transcriptional regulator involved in induction of numerous genes associated with angiogenesis and tumor growth, is highly increased by HPV E6. HIF-1α is a best-known target of the von Hippel-Lindau tumor suppressor (VHL) as an E3 ligase for degradation. In the present work, we found that HPV16 E6 promotes hypoxia induced Warburg effect through hindering the association of HIF-1α and VHL. This disassociation attenuates VHL-mediated HIF-1α ubiquitination and causes HIF-1α accumulation. These results suggest that oncoprotein E6 plays a major role in the regulation of Warburg effect and can be a valuable therapeutic target for HPV-related cancer.
SHSST Cyclodextrin Complex Prevents the Fibrosis Effect on CCl4-Induced Cirrhotic Cardiomyopathy in Rats through TGF-β Pathway Inhibition Effects
Authors: Yang Cheng-Hsun , Ting Wei-Jen , Day Cecilia , Ju Da-Tong , Yeh Yu-Lan , Chung Li-Chin , Tsai Fu-Jenn , Tsai Chang-Hai , Tsai Yuhsin , Huang Chih-Yang
Published: May 2014

Patients with liver cirrhosis also have subtle cardiac structure or function abnormalities. This cardiac dysfunction commonly occurs in 56% of waiting orthotopic liver transplantation (OLT) patients and is defined as cirrhotic cardiomyopathy (CCM). Up to now, there is no standard treatment because CCM does not have a solidly established diagnosis and is based on high clinical suspicion. The liver function of CCM is particularly limited, making patients vulnerable to more drug treatments. Here, we use silymarin (100 mg/kg/day), baicalein (30 mg/kg/day), San Huang Shel Shin Tang (SHSST, 30 mg/kg/day) and β-cyclodextrin modified SHSST (SHSSTc, 30 and 300 mg/kg/day) treatments for a CCl4-induced CCM rat model. The results show that silymarin, baicalein and SHSST treatments can only slightly reduce the collagen accumulation in CCM rat hearts. However, SHSSTc treatment protects the heart in CCM and significantly inhibits collagen acumination and the fibrosis regulating transforming growth factor-β (TGF-β) pathway expression. SHSSTc treatments further reduced the heart weight and the ratio between left ventricular weight (LVW) and tibia length (TL). This experimental data show that water solubility improved β-cyclodextrin modified Chinese herbal medicine formula (SHSSTc) can provide an excellent heart protection effect through TGF-β pathway inhibition.
Synthesis and Characterization of Novel Purpurinimides as Photosensitizers for Photodynamic Therapy
Authors: Cui Bing , Yoon Il , Li Jia , Lee Woo , Shim Young
Published: May 2014

A series of novel purpurinimides with long wavelength absorption were designed and synthesized to develop novel and potential photosensitizers. These compounds were investigated through reduction, oxidation, rearrangement reaction and amidation reactions of methyl pheophorbide a. They demonstrated a considerable bathochromic shift of the major absorption band in the red region of the optical spectrum (695–704 nm). Newly synthesized purpurinimides were screened for their antitumor activities, and showed higher photodynamic efficiency against A549 cell lines as compared to purpurin-18 methyl ester. The results revealed the novel purpurinimides could be potential photosensitizers.
The Roles of Vitamin A in the Regulation of Carbohydrate, Lipid, and Protein Metabolism
Authors: Chen Wei , Chen Guoxun
Published: May 2014

Currently, two-thirds of American adults are overweight or obese. This high prevalence of overweight/obesity negatively affects the health of the population, as obese individuals tend to develop several chronic diseases, such as type 2 diabetes and cardiovascular diseases. Due to obesity’s impact on health, medical costs, and longevity, the rise in the number of obese people has become a public health concern. Both genetic and environmental/dietary factors play a role in the development of metabolic diseases. Intuitively, it seems to be obvious to link over-nutrition to the development of obesity and other metabolic diseases. However, the underlying mechanisms are still unclear. Dietary nutrients not only provide energy derived from macronutrients, but also factors such as micronutrients with regulatory roles. How micronutrients, such as vitamin A (VA; retinol), regulate macronutrient homeostasis is still an ongoing research topic. As an essential micronutrient, VA plays a key role in the general health of an individual. This review summarizes recent research progress regarding VA’s role in carbohydrate, lipid, and protein metabolism. Due to the large amount of information regarding VA functions, this review focusses on metabolism in metabolic active organs and tissues. Additionally, some perspectives for future studies will be provided.
International Diversification Versus Domestic Diversification: Mean-Variance Portfolio Optimization and Stochastic Dominance Approaches
Authors: Abid Fathi , Leung Pui , Mroua Mourad , Wong Wing
Published: May 2014

This paper applies the mean-variance portfolio optimization (PO) approach and the stochastic dominance (SD) test to examine preferences for international diversification versus domestic diversification from American investors’ viewpoints. Our PO results imply that the domestic diversification strategy dominates the international diversification strategy at a lower risk level and the reverse is true at a higher risk level. Our SD analysis shows that there is no arbitrage opportunity between international and domestic stock markets; domestically diversified portfolios with smaller risk dominate internationally diversified portfolios with larger risk and vice versa; and at the same risk level, there is no difference between the domestically and internationally diversified portfolios. Nonetheless, we cannot find any domestically diversified portfolios that stochastically dominate all internationally diversified portfolios, but we find some internationally diversified portfolios with small risk that dominate all the domestically diversified portfolios.
Optical Properties of Electrically Tunable Two-Dimensional Photonic Lattice Structures Formed in a Holographic Polymer-Dispersed Liquid Crystal Film: Analysis and Experiment
Authors: Miki Mayu , Ohira Ryuichiro , Tomita Yasuo
Published: May 2014

We report on theoretical and experimental investigations of optical wave propagations in two-dimensional photonic lattice structures formed in a holographic polymer-dispersed liquid crystal (HPDLC) film. In the theoretical analysis we employed the 2×2 matrix formulation and the statistical thermodynamics model to analyze the formation of anisotropic photonic lattice structures by holographic polymerization. The influence of multiple reflections inside an HPDLC film on the formed refractive index distribution was taken into account in the analysis. In the experiment we fabricated two-dimensional photonic lattice structures in an HPDLC film under three-beam interference holographic polymerization and performed optical measurements of spectral transmittances and wavelength dispersion. We also demonstrated the electrical control capability of the fabricated photonic lattice structure and its dependence on incident wave polarization. These measured results were compared with the calculated ones by means of photonic band and beam propagation calculations.
A Review of Dissimilar Welding Techniques for Magnesium Alloys to Aluminum Alloys
Authors: Liu Liming , Ren Daxin , Liu Fei
Published: May 2014

Welding of dissimilar magnesium alloys and aluminum alloys is an important issue because of their increasing applications in industries. In this document, the research and progress of a variety of welding techniques for joining dissimilar Mg alloys and Al alloys are reviewed from different perspectives. Welding of dissimilar Mg and Al is challenging due to the formation of brittle intermetallic compound (IMC) such as Mg17Al12 and Mg2Al3. In order to increase the joint strength, three main research approaches were used to eliminate or reduce the Mg-Al intermetallic reaction layer. First, solid state welding techniques which have a low welding temperature were used to reduce the IMCs. Second, IMC variety and distribution were controlled to avoid the degradation of the joining strength in fusion welding. Third, techniques which have relatively controllable reaction time and energy were used to eliminate the IMCs. Some important processing parameters and their effects on weld quality are discussed, and the microstructure and metallurgical reaction are described. Mechanical properties of welds such as hardness, tensile, shear and fatigue strength are discussed. The aim of the report is to review the recent progress in the welding of dissimilar Mg and Al to provide a basis for follow-up research.
Traveling Wave Solutions of Reaction-Diffusion Equations Arising in Atherosclerosis Models
Authors: Apreutesei Narcisa
Published: May 2014

In this short review article, two atherosclerosis models are presented, one as a scalar equation and the other one as a system of two equations. They are given in terms of reaction-diffusion equations in an infinite strip with nonlinear boundary conditions. The existence of traveling wave solutions is studied for these models. The monostable and bistable cases are introduced and analyzed.
Comparative Study on the Antioxidant and Anti-Toxoplasma Activities of Vanillin and Its Resorcinarene Derivative
Authors: Oliveira Claudio , Meurer Ywlliane , Oliveira Marianne , Medeiros Wendy , Silva Francisco , Brito Ana , Pontes Daniel , Andrade-Neto Valter
Published: May 2014

A resorcinarene derivative of vanillin, resvan, was synthesized and characterized by spectroscopic techniques. We measured the cytotoxicity (in vivo and in vitro), antioxidant and anti-Toxoplasma activities of vanillin and the resorcinarene compound. Here we show that vanillin has a dose-dependent behavior with IC50 of 645 µg/mL through an in vitro cytotoxicity assay. However, we could not observe any cytotoxic response at higher concentrations of resvan (IC50 > 2,000 µg/mL). The in vivo acute toxicity assays of vanillin and resvan exhibited a significant safety margin indicated by a lack of systemic and behavioral toxicity up to 300 mg/kg during the first 30 min, 24 h or 14 days after administration. The obtained derivative showed greater antioxidative activity (84.9%) when comparing to vanillin (19.4%) at 1,000 μg/mL. In addition, vanillin presents anti-Toxoplasma activity, while resvan does not show that feature. Our findings suggest that this particular derivative has an efficient antioxidant activity and a negligible cytotoxic effect, making it a potential target for further biological investigations.
Behavior of FRP-Confined Concrete-Filled Steel Tube Columns
Authors: Lu Yiyan , Li Na , Li Shan
Published: May 2014

This paper presents the results of an experimental study into the behavior of concrete-filled steel tube columns confined by fiber-reinforced polymer (FRP). Eleven columns were tested to investigate the effects of the FRP layer number, the thickness of the steel tube and concrete strength on their load capacity and axial deformation capacity. The experimental results indicated that the FRP wrap can effectively confine the concrete expansion and delay the local buckling of the steel tube. Both the load capacity and the axial deformation capacity of concrete-filled steel tube columns can be substantially enhanced with FRP confinement. A model is proposed to predict the load capacity of the FRP-confined concrete-filled steel tube columns. The predicted results are generally in good agreement with the experimental ones obtained in this study and in the literature.
Capability of Integrated MODIS Imagery and ALOS for Oil Palm, Rubber and Forest Areas Mapping in Tropical Forest Regions
Authors: Razali Sheriza , Marin Arnaldo , Nuruddin Ahmad , Shafri Helmi , Hamid Hazandy
Published: May 2014

Various classification methods have been applied for low resolution of the entire Earth’s surface from recorded satellite images, but insufficient study has determined which method, for which satellite data, is economically viable for tropical forest land use mapping. This study employed Iterative Self Organizing Data Analysis Techniques (ISODATA) and K-Means classification techniques to classified Moderate Resolution Imaging Spectroradiometer (MODIS) Surface Reflectance satellite image into forests, oil palm groves, rubber plantations, mixed horticulture, mixed oil palm and rubber and mixed forest and rubber. Even though frequent cloud cover has been a challenge for mapping tropical forests, our MODIS land use classification map found that 2008 ISODATA-1 performed well with overall accuracy of 94%, with the highest Producer’s Accuracy of Forest with 86%, and were consistent with MODIS Land Cover 2008 (MOD12Q1), respectively. The MODIS land use classification was able to distinguish young oil palm groves from open areas, rubber and mature oil palm plantations, on the Advanced Land Observing Satellite (ALOS) map, whereas rubber was more easily distinguished from an open area than from mixed rubber and forest. This study provides insight on the potential for integrating regional databases and temporal MODIS data, in order to map land use in tropical forest regions.
Vocalizing the Angels of Mons: Audio Dramas as Propaganda in the Great War of 1914 to 1918
Authors: Crook Tim
Published: May 2014

Sound drama production prior to the onset of the “Radio Age” underwent a pioneering development during the Great War. This was achieved by the making, publication and distribution of short audio dramas acted with sound effects and music in front of early microphones and released in the form of 78 rpm phonograph discs. Entertaining storytelling through dramatic performance was mobilized for the purposes of improving recruitment and disseminating patriotic endorsement recordings. This article focuses on the sound dramatization of the myth of “The Angels of Mons” released by Regal in 1915. The recording is examined as a text for its significance in terms of propaganda, style of audio-drama, and any cultural role it may have played in the media of the First World War. The Regal disc was an example of what was described at the time as “descriptive sketches.” This article explores why a sound phonograph was used to dramatize the myth that angels intervened to assist the British Expeditionary Force to resist the German Army invading France through Belgium in 1914. A number of historians have discussed the First World War as being a theatre for the first modern media war, in which the process of propaganda was modernized. To what extent does “The Angels of Mons” phonograph and the genre of descriptive sketches support this analysis? Does this short sound drama play have any relevance to the cultural phenomena of spiritualism, modernism and patriotic Christianity identified as being important during the Great War period?
A Novel Adenovirus in Chinstrap Penguins (Pygoscelis antarctica) in Antarctica
Authors: Lee Sook-Young , Kim Jeong-Hoon , Park Yon , Shin Ok , Kim Hankyeom , Choi Han-Gu , Song Jin-Won
Published: May 2014

Adenoviruses (family Adenoviridae) infect various organ systems and cause diseases in a wide range of host species. In this study, we examined multiple tissues from Chinstrap penguins (Pygoscelis antarctica), collected in Antarctica during 2009 and 2010, for the presence of novel adenoviruses by PCR. Analysis of a 855-bp region of the hexon gene of a newly identified adenovirus, designated Chinstrap penguin adenovirus 1 (CSPAdV-1), showed nucleotide (amino acid) sequence identity of 71.8% (65.5%) with South Polar skua 1 (SPSAdV-1), 71% (70%) with raptor adenovirus 1 (RAdV-1), 71.4% (67.6%) with turkey adenovirus 3 (TAdV-3) and 61% (61.6%) with frog adenovirus 1 (FrAdV-1). Based on the genetic and phylogenetic analyses, CSPAdV-1 was classified as a member of the genus, Siadenovirus. Virus isolation attempts from kidney homogenates in the MDTC-RP19 (ATCC® CRL-8135™) cell line were unsuccessful. In conclusion, this study provides the first evidence of new adenovirus species in Antarctic penguins.
Therapeutic Potential of MicroRNA Let-7: Tumor Suppression or Impeding Normal Stemness
Authors: Chiu Shao-Chih , Chung Hao-Yu , Cho Der-Yang , Chan Tzu-Min , Liu Ming-Chao , Huang Hiang-Ming , Li Tsyng-You , Lin Jian-Yong , Chou Pei-Chang , Fu Ru-Huei , Yang Wen-Kuang , Harn Horng-Jyh , Lin Shinn-Zong
Published: May 2014

The first microRNA, let-7, and its family were discovered in Caenorhabditis elegans and are functionally conserved from worms to humans in the regulation of embryonic development and stemness. The let-7 family has been shown to have an essential role in stem cell differentiation and tumor-suppressive activity. Deregulating expression of let-7 is commonly reported in many human cancers. Emerging evidence has accumulated and suggests that reestablishment of let-7 in tumor cells is a valuable therapeutic strategy. However, findings reach beyond tumor therapeutics and may impinge on stemness and differentiation of stem cells. In this review, we discuss the role of let-7 in development and differentiation of normal adult stem/progenitor cells and offer a viewpoint of the association between deregulated let-7 expression and tumorigenesis. The regulation of let-7 expression, cancer-relevant let-7 targets, and the application of let-7 are highlighted.
Spinal Cord Regeneration
Authors: Young Wise
Published: May 2014

Three theories of regeneration dominate neuroscience today, all purporting to explain why the adult central nervous system (CNS) cannot regenerate. One theory proposes that Nogo, a molecule expressed by myelin, prevents axonal growth. The second theory emphasizes the role of glial scars. The third theory proposes that chondroitin sulfate proteoglycans (CSPGs) prevent axon growth. Blockade of Nogo, CSPG, and their receptors indeed can stop axon growth in vitro and improve functional recovery in animal spinal cord injury (SCI) models. These therapies also increase sprouting of surviving axons and plasticity. However, many investigators have reported regenerating spinal tracts without eliminating Nogo, glial scar, or CSPG. For example, many motor and sensory axons grow spontaneously in contused spinal cords, crossing gliotic tissue and white matter surrounding the injury site. Sensory axons grow long distances in injured dorsal columns after peripheral nerve lesions. Cell transplants and treatments that increase cAMP and neurotrophins stimulate motor and sensory axons to cross glial scars and to grow long distances in white matter. Genetic studies deleting all members of the Nogo family and even the Nogo receptor do not always improve regeneration in mice. A recent study reported that suppressing the phosphatase and tensin homolog (PTEN) gene promotes prolific corticospinal tract regeneration. These findings cannot be explained by the current theories proposing that Nogo and glial scars prevent regeneration. Spinal axons clearly can and will grow through glial scars and Nogo-expressing tissue under some circumstances. The observation that deleting PTEN allows corticospinal tract regeneration indicates that the PTEN/AKT/mTOR pathway regulates axonal growth. Finally, many other factors stimulate spinal axonal growth, including conditioning lesions, cAMP, glycogen synthetase kinase inhibition, and neurotrophins. To explain these disparate regenerative phenomena, I propose that the spinal cord has evolved regenerative mechanisms that are normally suppressed by multiple extrinsic and intrinsic factors but can be activated by injury, mediated by the PTEN/AKT/mTOR, cAMP, and GSK3b pathways, to stimulate neural growth and proliferation.
ADSC Therapy in Neurodegenerative Disorders
Authors: Chan Tzu-Min , Chen Julia Yi-Ru , Ho Li-Ing , Lin Hui-Ping , Hsueh Kuo-Wei , Liu Demeral David , Chen Yi-Hung , Hsieh An-Cheng , Tsai Nu-Man , Hueng Dueng-Yuan , Tsai Sheng-Tzeng , Chou Pei-Wen , Lin Shinn-Zong , Harn Horng-Jyh
Published: May 2014

Neurodegenerative disorders, chronic diseases that can severely affect the patient's daily life, include amyotrophic lateral sclerosis, Parkinson's, Alzheimer's, and Huntington's diseases. However, these diseases all have the common characteristic that they are due to degenerative irreversibility, and thus no efficient drugs or therapy methods can mitigate symptoms completely. Stem cell therapy, such as adipose tissue-derived stem cells (ADSCs), is a promising treatment for incurable disorders. In this review, we summarized the previous studies using ADSCs to treat neurodegenerative disorders, as well as their therapeutic mechanisms. We also suggested possible expectations for future human clinical trials involving minimized intracerebroventricular combined with intravenous administration, using different cell lineages to finish complementary therapy as well as change the extracellular matrix to create a homing niche. Depending on successful experiments in relevant neurodegenerative disorders models, this could form the theoretical basis for future human clinical trials.
Polyglutamine (PolyQ) Diseases: Genetics to Treatments
Authors: Fan Hueng-Chuen , Ho Li-Ing , Chi Ching-Shiang , Chen Shyi-Jou , Peng Giia-Sheun , Chan Tzu-Min , Lin Shinn-Zong , Harn Horng-Jyh
Published: May 2014

The polyglutamine (polyQ) diseases are a group of neurodegenerative disorders caused by expanded cytosine– adenine–guanine (CAG) repeats encoding a long polyQ tract in the respective proteins. To date, a total of nine polyQ disorders have been described: six spinocerebellar ataxias (SCA) types 1, 2, 6, 7, 17; Machado–Joseph disease (MJD/SCA3); Huntington's disease (HD); dentatorubral pallidoluysian atrophy (DRPLA); and spinal and bulbar muscular atrophy, X-linked 1 (SMAX1/SBMA). PolyQ diseases are characterized by the pathological expansion of CAG trinucleotide repeat in the translated region of unrelated genes. The translated polyQ is aggregated in the degenerated neurons leading to the dysfunction and degeneration of specific neuronal subpopulations. Although animal models of polyQ disease for understanding human pathology and accessing disease-modifying therapies in neurodegenerative diseases are available, there is neither a cure nor prevention for these diseases, and only symptomatic treatments for polyQ diseases currently exist. Long-term pharmacological treatment is so far disappointing, probably due to unwanted complications and decreasing drug efficacy. Cellular transplantation of stem cells may provide promising therapeutic avenues for restoration of the functions of degenerative and/or damaged neurons in polyQ diseases.
Biogeography and phylogenomics of New World Bambusoideae (Poaceae), revisited
Authors: Burke Sean V. , Clark Lynn G. , Triplett Jimmy K. , Grennan Colin P. , Duvall Melvin R.
Published: May 2014

• Premise of the study: New World Bambusoideae have only recently been studied in a phylogenomic context. Plastome sequences were determined and analyzed from Arundinaria appalachiana, A. tecta, and Olyra latifolia, to refine our knowledge of their evolution and historical biogeography. A correction is noted regarding an error in an earlier report on the biogeography of Cryptochloa.

• Methods: Single‐end DNA libraries were prepared and sequenced on the Illumina platform. Complete plastomes were assembled and analyzed with 13 other Poaceae.

• Key results: Complete sampling in Arundinaria and an additional species of Olyreae gave a more detailed picture of their evolution/historical biogeography. Phylogenomic analyses indicated that the first major divergence in Arundinaria occurred around 2.3 to 3.2 mya and that Arundinaria tecta and A. appalachiana diverged from their common ancestor around 0.57 to 0.82 mya. Estimates of the divergence of Olyra latifolia from Cryptochloa strictiflora ranged from 14.6 to 20.7 mya. The age of the stem node of Olyreae ranged from an estimated 26.9 to 38.2 mya.

• Conclusions: Estimates of divergences in Arundinaria can be correlated with paleoclimatic events including an early Pliocene warming, subsequent cooling, and North American glaciations. Discriminating between alternate evolutionary/biogeographic scenarios in Olyreae is challenging.
Microsatellite markers for the invasive species Bidens alba (Asteraceae)
Authors: Lu Yong‐Bin , Huang Dong‐Ling , Wang Xie , Wu Zheng‐Jun , Tang Shao‐Qing
Published: May 2014

• Premise of the study: Microsatellite markers were developed in the invasive species Bidens alba (Asteraceae) to assess its population structure and to facilitate tracking its expansion in China.

• Methods and Results: Using 454 pyrosequencing, 20 microsatellite primer sets were developed for B. alba. The markers were tested on one population of B. alba (30 individuals) and one population of the closely related B. pilosa (30 individuals) in China. For B. alba, all of the markers were polymorphic, and the number of alleles per locus ranged from three to 32. The expected heterozygosity values were from 0.3787 to 0.9284, and the Shannon–Wiener index was from 0.6796 to 2.8401.

• Conclusions: These markers will be useful for investigating the genetic structure, genetic diversity, and invasion dynamics of B. alba and will also be useful in studies of B. pilosa.
High activity of Ag-doped Cd0.1Zn0.9S photocatalyst prepared by the hydrothermal method for hydrogen production under visible-light irradiation
Authors: Yuliati Leny , Kimi Melody , Shamsuddin Mustaffa
Published: May 2014

Background: The hydrothermal method was used as a new approach to prepare a series of Ag-doped Cd0.1Zn0.9S photocatalysts. The effect of Ag doping on the properties and photocatalytic activity of Cd0.1Zn0.9S was studied for the hydrogen production from water reduction under visible light irradiation.

Results: Compared to the series prepared by the co-precipitation method, samples prepared by the hydrothermal method performed with a better photocatalytic activity. The sample with the optimum amount of Ag doping showed the highest hydrogen production rate of 3.91 mmol/h, which was 1.7 times higher than that of undoped Cd0.1Zn0.9S. With the Ag doping, a red shift in the optical response was observed, leading to a larger portion of the visible light absorption than that of without doping. In addition to the larger absorption in the visible-light region, the increase in photocatalytic activity of samples with Ag doping may also come from the Ag species facilitating electron–hole separation.

Conclusion: This study demonstrated that Ag doping is a promising way to enhance the activity of Cd0.1Zn0.9S photocatalyst.
A new building block for DNA network formation by self-assembly and polymerase chain reaction
Authors: Bußkamp Holger , Keller Sascha , Robotta Marta , Drescher Malte , Marx Andreas
Published: May 2014

The predictability of DNA self-assembly is exploited in many nanotechnological approaches. Inspired by naturally existing self-assembled DNA architectures, branched DNA has been developed that allows self-assembly to predesigned architectures with dimensions on the nanometer scale. DNA is an attractive material for generation of nanostructures due to a plethora of enzymes which modify DNA with high accuracy, providing a toolbox for many different manipulations to construct nanometer scaled objects. We present a straightforward synthesis of a rigid DNA branching building block successfully used for the generation of DNA networks by self-assembly and network formation by enzymatic DNA synthesis. The Y-shaped 3-armed DNA construct, bearing 3 primer strands is accepted by Taq DNA polymerase. The enzyme uses each arm as primer strand and incorporates the branched construct into large assemblies during PCR. The networks were investigated by agarose gel electrophoresis, atomic force microscopy, dynamic light scattering, and electron paramagnetic resonance spectroscopy. The findings indicate that rather rigid DNA networks were formed. This presents a new bottom-up approach for DNA material formation and might find applications like in the generation of functional hydrogels.