bluuebunny/crossref_metadata_2025 huggingface dataset:
Understanding exopolysaccharide byproduct formation in Komagataella phaffii fermentation processes for recombinant protein production
Authors: Steimann Thomas , Heite Zoe , Germer Andrea , Blank Lars Mathias , Büchs Jochen , Mann Marcel , Magnus Jørgen Barsett
Published: May 2024

Background

Komagataella phaffii (Pichia pastoris) has emerged as a common and robust biotechnological platform organism, to produce recombinant proteins and other bioproducts of commercial interest. Key advantage of K. phaffii is the secretion of recombinant proteins, coupled with a low host protein secretion. This facilitates downstream processing, resulting in high purity of the target protein. However, a significant but often overlooked aspect is the presence of an unknown polysaccharide impurity in the supernatant. Surprisingly, this impurity has received limited attention in the literature, and its presence and quantification are rarely addressed.

Results

This study aims to quantify this exopolysaccharide in high cell density recombinant protein production processes and identify its origin. In stirred tank fed-batch fermentations with a maximal cell dry weight of 155 g/L, the polysaccharide concentration in the supernatant can reach up to 8.7 g/L. This level is similar to the achievable target protein concentration. Importantly, the results demonstrate that exopolysaccharide production is independent of the substrate and the protein production process itself. Instead, it is directly correlated with biomass formation and proportional to cell dry weight. Cell lysis can confidently be ruled out as the source of this exopolysaccharide in the culture medium. Furthermore, the polysaccharide secretion can be linked to a mutation in the HOC1 gene, featured by all derivatives of strain NRRL Y-11430, leading to a characteristic thinner cell wall.

Conclusions

This research sheds light on a previously disregarded aspect of K. phaffii fermentations, emphasizing the importance of monitoring and addressing the exopolysaccharide impurity in biotechnological applications, independent of the recombinant protein produced.
A simple, field-applicable method to increase the infectivity of wild isolates of Plasmodium falciparum to mosquito vectors
Authors: Ouattara Seydou Bienvenu , Hien Domonbabele F. D. S. , Nao Ekôbié T. , Paré Prisca S. L. , Guissou Edwige , Cohuet Anna , Morlais Isabelle , Yerbanga Rakiswendé S. , Dabiré Kounbobr R. , Ouédraogo Jean Bosco , Mouline Karine , Lefèvre Thierry
Published: May 2024

Background

The direct membrane feeding assay (DMFA), whereby gametocyte-infected blood is collected from human donors and from which mosquitoes feed through a membrane, is proving essential for assessing parameters influencing Plasmodium transmission potential in endemic countries. The success of DMFAs is closely tied to gametocyte density in the blood, with relatively high gametocytaemia ensuring optimal infection levels in mosquitoes. As transmission intensity declines with control efforts, the occurrence of asymptomatic individuals with low gametocyte densities, who can significantly contribute to the infectious reservoir, is increasing. This poses a limitation to studies relying on the experimental infection of large numbers of mosquitoes with natural isolates of Plasmodium. A simple, field-applicable method is presented for improving parasite infectivity by concentrating Plasmodium falciparum gametocytes.

Methods

Anopheles gambiae received one of the following 5 blood treatments through DMFA: (i) whole blood (WB) samples from naturally-infected donors; (ii) donor blood whose plasma was replaced with the same volume of Plasmodium-naive AB + serum (1:1 control); (iii) plasma replaced with a volume of malaria-naïve AB + serum equivalent to half (1:1/2), or to a quarter (1:1/4), of the initial plasma volume; and (v) donor blood whose plasma was fully removed (RBC). The experiment was repeated 4 times using 4 distinct wild parasite isolates. Seven days post-infection, a total of 1,095 midguts were examined for oocyst presence.

Results

Substituting plasma with reduced amounts (1:1/2 and 1:1/4) of Plasmodium-naive AB + serum led to a 31% and 17% increase of the mosquito infection rate and to a 85% and 308% increase in infection intensity compared to the 1:1 control, respectively. The full removal of plasma (RBC) reduced the infection rate by 58% and the intensity by 64% compared to the 1:1 control. Reducing serum volumes (1:1/2; 1:1/4 and RBC) had no impact on mosquito feeding rate and survival when compared to the 1:1 control.

Conclusions

Concentrating gametocytic blood by replacing natural plasma by lower amount of naive serum can enhance the success of mosquito infection. In an area with low gametocyte density, this simple and practical method of parasite concentration can facilitate studies on human-to-mosquito transmission such as the evaluation of transmission-blocking interventions.
Time Kinetics and prognosis roles of calcitonin after surgery for medullary thyroid carcinoma
Authors: Guo Fengli , Fu Guiming , Li Fangxuan , Hua Yitong , Wang Zhongyu , Zheng Xiangqian , Zhao Jingzhu , Gao Ming
Published: May 2024

Background

Medullary thyroid carcinoma (MTC) is a malignant tumor with low incidence. Currently, most studies have focused on the prognostic risk factors of MTC, whatever, time kinetic and risk factors related to calcitonin normalization (CN) and biochemical persistence/recurrence (BP) are yet to be elucidated.

Methods

A retrospective study was conducted for 190 MTC patients. Risk factors related to calcitonin normalization (CN) and biochemical persistence/recurrence (BP) were analyzed. The predictors of calcitonin normalization time (CNT) and biochemical persistent/recurrent time (BPT) were identified. Further, the prognostic roles of CNT and BPT were also demonstrated.

Results

The 5- and 10-year DFS were 86.7% and 70.2%, respectively. The 5- and 10-year OS were 97.6% and 78.8%, respectively. CN was achieved in 120 (63.2%) patients, whereas BP was presented in 76 (40.0%) patients at the last follow up. After curative surgery, 39 (32.5%) and 106 (88.3%) patients achieved CN within 1 week and 1 month. All patients who failed to achieve CN turned to BP over time and 32/70 of them developed structural recurrence. The median time of CNT and BPT was 1 month (1 day to 84 months) and 6 month (3 day to 63months), respectively. LNR > 0.23 and male gender were independent predictors for CN and BP. LNR > 0.23 (Hazard ratio (HR), 0.24; 95% CI,0.13–0.46; P < 0.01) and male gender (HR, 0.65; 95% CI, 0.42–0.99; P = 0.045) were independent predictors for longer CNT. LNR > 0.23 (HR,5.10; 95% CI,2.15–12.11; P < 0.01) was still the strongest independent predictor followed by preoperative serum Ctn > 1400ng/L (HR,2.34; 95% CI,1.29–4.25; P = 0.005) for shorter BPT. In survival analysis, primary tumor size > 2 cm (HR, 5.81; 95% CI,2.20-15.38; P < 0.01), CNT > 1 month (HR, 5.69; 95% CI, 1.17–27.61; P = 0.031) and multifocality (HR, 3.10; 95% CI, 1.45–6.65; P = 0.004) were independent predictor of DFS.

Conclusion

Early changes of Ctn after curative surgery can predict the long-term risks of biochemical and structural recurrence, which provide a useful real-time prognostic information. LNR significantly affect the time kinetic of biochemical prognosis. Tumor burden and CNT play a crucial role in MTC survival, the intensity of follow-up must be tailored accordingly.
Novel genome-wide DNA methylation profiling reveals distinct epigenetic landscape, prognostic model and cellular composition of early-stage lung adenocarcinoma
Authors: Gan Junwen , Huang Meng , Wang Weishi , Fu Guining , Hu Mingyuan , Zhong Hongcheng , Ye Xin , Cao Qingdong
Published: May 2024

Background

Lung adenocarcinoma (LUAD) has been a leading cause of cancer-related mortality worldwide. Early intervention can significantly improve prognosis. DNA methylation could occur in the early stage of tumor. Comprehensive understanding the epigenetic landscape of early-stage LUAD is crucial in understanding tumorigenesis.

Methods

Enzymatic methyl sequencing (EM-seq) was performed on 23 tumors and paired normal tissue to reveal distinct epigenetic landscape, for compared with The Cancer Genome Atlas (TCGA) 450K methylation microarray data. Then, an integrative analysis was performed combined with TCGA LUAD RNA-seq data to identify significant differential methylated and expressed genes. Subsequently, the prognostic risk model was constructed and cellular composition was analyzed.

Results

Methylome analysis of EM-seq comparing tumor and normal tissues identified 25 million cytosine-phosphate-guanine (CpG) sites and 30,187 differentially methylated regions (DMR) with a greater number of untraditional types. EM-seq identified a significantly higher number of CpG sites and DMRs compared to the 450K microarray. By integrating the differentially methylated genes (DMGs) with LUAD-related differentially expressed genes (DEGs) from the TCGA database, we constructed prognostic model based on six differentially methylated-expressed genes (MEGs) and verified our prognostic model in GSE13213 and GSE42127 dataset. Finally, cell deconvolution based on the in-house EM-seq methylation profile was used to estimate cellular composition of early-stage LUAD.

Conclusions

This study firstly delves into novel pattern of epigenomic DNA methylation and provides a multidimensional analysis of the role of DNA methylation revealed by EM-seq in early-stage LUAD, providing distinctive insights into its potential epigenetic mechanisms.
From isolation to revival: trade recovery amid global health crises
Authors: Yang Lijuan
Published: May 2024

Background

The COVID-19 pandemic has highlighted the importance of designing effective trade recovery measures in response to global health events (GHEs). This study combines international trade risk management theory and multi-case comparative analysis of past GHEs to present a theoretical framework for designing national trade recovery measures for future events.

Results

The research finds that during GHEs, trade risks shift to fundamental uncertainty, requiring spatial–temporal-subject dimension recovery measures. The study suggests changing the focus of trade recovery policy design from emergency-oriented and single-dimension measures to reserve-oriented and enduring-effect measures of comprehensive dimensions at micro- and macroeconomic levels.

Conclusion

The study contributes to the debate on managing trade risks in times of crisis, where there is a need to develop effective trade recovery measures that account for the complexities of global trade and the unique challenges of GHEs. The findings provide practical guidance for trade officials and policymakers to design measures in response to GHEs to improve a country’s overall trade recovery.
Development of a workplace breastfeeding support practice model in South Africa
Authors: Daniels Lynette Carmen , Mbhenyane Xikombiso Gertrude , Du Plessis Lisanne Monica
Published: May 2024

Background

Globally, mothers have identified work as one of the main obstacles to exclusive and continued breastfeeding. The support a woman receives in her workplace in terms of workplace arrangements can be critical to enable women to continue breastfeeding. This study aimed to develop and assess the face validity of a practice model to support exclusive and continued breastfeeding in workplaces in the Western Cape, South Africa.

Methods

An explanatory, sequential, mixed-method research design, was conducted (June 2017 to March 2019) in three distinct phases. Phase one employed a quantitative, descriptive, cross-sectional study design. Phase 2 used a qualitative, multiple case study. Phase three involved the development and face validity of a practice model to support exclusive breastfeeding in workplaces. The face validity included two Delphi rounds for experts to provide input on the draft practice model. This paper will only report on phase 3 of the study. The practice model was developed, drawing on the analysis of data from phases one and two and using programme theory approaches and a logic model.

Results

The practice model was positively perceived. Participants viewed it as informative, well designed and easy to follow, even for those not knowledgeable about the subject. It was viewed as an ideal tool, if accompanied by some training. Participants were positive that the model would be feasible and most commended the tiered approach to implementation. They felt that workplaces would be more open to a step-by-step approach to implementation and if only a few activities are implemented it would be a start to make the work environment more conducive for breastfeeding employees. There were mixed opinions regarding commitment; a few participants mentioned commitment as a challenge they anticipated in the male-dominant environments in which they worked. The provision of space for breastfeeding at the workplace was also highlighted as a potential challenge.

Conclusions

Advocacy around creating an enabling workplace environment for breastfeeding is needed. The practice model has the potential to be internationally relevant, locally applied and may be of particular use to workplaces that want to initiate and/or strengthen breastfeeding support.
Evaluating lumbar disc degeneration by MRI quantitative metabolic indicators: the perspective of factor analysis
Authors: Zheng Boxin , Ouyang Lin , Shi Jianhua , Shen Xiaochan , Lei Hanbin
Published: May 2024

Purpose

This study aimed to investigate an early diagnostic method for lumbar disc degeneration (LDD) and improve its diagnostic accuracy.

Methods

Quantitative biomarkers of the lumbar body (LB) and lumbar discs (LDs) were obtained using nuclear magnetic resonance (NMR) detection technology. The diagnostic weights of each biological metabolism indicator were screened using the factor analysis method.

Results

Through factor analysis, common factors such as the LB fat fraction, fat content, and T2* value of LDs were identified as covariates for the diagnostic model for the evaluation of LDD. This model can optimize the accuracy and reliability of LDD diagnosis.

Conclusion

The application of biomarker quantification methods based on NMR detection technology combined with factor analysis provides an effective means for the early diagnosis of LDD, thereby improving diagnostic accuracy and reliability.
Effects of various load magnitudes on ACL: an in vitro study using adolescent porcine stifle joints
Authors: Koh Jason , Mungalpara Nirav , Kim Sunjung , Bedi Asheesh , Hutchinson Mark , Amirouche Farid
Published: May 2024

Introduction

The escalating incidence of anterior cruciate ligament (ACL) injuries, particularly among adolescents, is a pressing concern. The study of ACL biomechanics in this demographic presents challenges due to the scarcity of cadaveric specimens. This research endeavors to validate the adolescent porcine stifle joint as a fitting model for ACL studies.

Methods

We conducted experiments on 30 fresh porcine stifle knee joints. (Breed: Yorkshire, Weight: avg 90 lbs, Age Range: 2–4 months). They were stored at − 22 °C and a subsequent 24-h thaw at room temperature before being prepared for the experiment. These joints were randomly assigned to three groups. The first group served as a control and underwent only the load-to-failure test. The remaining two groups were subjected to 100 cycles, with forces of 300N and 520N, respectively. The load values of 300N and 520N correspond to three and five times the body weight (BW) of our juvenile porcine, respectively.

Result

The 520N force demonstrated a higher strain than the 300N, indicating a direct correlation between ACL strain and augmented loads. A significant difference in load-to-failure (p = 0.014) was observed between non-cyclically loaded ACLs and those subjected to 100 cycles at 520N. Three of the ten samples in the 520N group failed before completing 100 cycles. The ruptured ACLs from these tests closely resembled adolescent ACL injuries in detachment patterns. ACL stiffness was also measured post-cyclical loading by applying force and pulling the ACL at a rate of 1 mm per sec. Moreover, ACL stiffness measurements decreased from 152.46 N/mm in the control group to 129.42 N/mm after 100 cycles at 300N and a more significant drop to 86.90 N/mm after 100 cycles at 520N. A one-way analysis of variance (ANOVA) and t-test were chosen for statistical analysis.

Conclusions

The porcine stifle joint is an appropriate model for understanding ACL biomechanics in the skeletally immature demographic. The results emphasize the ligament’s susceptibility to injury under high-impact loads pertinent to sports activities. The study advocates for further research into different loading scenarios and the protective role of muscle co-activation in ACL injury prevention.
Impact of treadmill running on distal femoral cartilage thickness: a cross-sectional study of professional athletes and healthy controls
Authors: Azami Pouria , Ashraf Alireza , Yousefi Omid , Hosseinpour Alireza , Nasiri Aref
Published: May 2024

Purpose

This present study aimed to assess the impact of treadmill running on distal femoral cartilage thickness.

Methods

Professional athletes aged 20 to 40 years with a history of treadmill running (minimum 75 min per week for the past three months or more) and age-, sex-, and body mass index (BMI)-matched healthy controls were recruited. Demographics and clinical features of participants were recorded. Athletes were divided into subgroup 1 with less than 12 months of treadmill running and subgroup 2 with 12 months or more of treadmill running. Distal femoral cartilage thicknesses were measured at the midpoints of the right medial condyle (RMC), right intercondylar area (RIA), right lateral condyle (RLC), left medial condyle (LMC), left intercondylar area (LIA), and left lateral condyle (LLC) via ultrasonography.

Result

A total of 72 athletes (mean age: 29.6 ± 6.6 years) and 72 controls (mean age: 31.9 ± 6.7 years) were enrolled. Athletes had significantly thinner cartilages in the RLC (2.21 ± 0.38 vs. 2.39 ± 0.31 cm, p = 0.002), LLC (2.28 ± 0.37 vs. 2.46 ± 0.35 cm, p = 0.004), and LMC (2.28 ± 0.42 vs. 2.42 ± 0.36 cm, p = 0.039) compared with the control group. Furthermore, cartilage thickness was significantly thinner in subgroup 2 athletes compared with the control group in the RLC (2.13 ± 0.34 vs. 2.39 ± 0.31 cm, p = 0.001), LLC (2.22 ± 0.31 vs. 2.46 ± 0.35 cm, p = 0.005), and LMC (2.21 ± 0.46 vs. 2.42 ± 0.36 cm, p = 0.027); however, subgroup 1 athletes did not have such differences. There was a weak negative correlation between total months of treadmill running and cartilage thickness in the RLC (r = − 0.0236, p = 0.046) and LLC (r = − 0.0233, p = 0.049). No significant correlation was found between the distal femoral cartilage thickness at different sites and the patients’ demographic features, including age, BMI, speed and incline of treadmill running, and minutes of running per session and week (p > 0.05).

Conclusion

Compared with healthy controls, professional athletes with a history of long-term high-intensity treadmill running had thinner femoral cartilages. The duration (months) of treadmill running was weakly negatively correlated with distal femoral cartilage thickness. Longitudinal studies with prolonged follow-ups are needed to clarify how treadmill running affects femoral cartilage thickness in athletes.
Chen inequalities on warped product Legendrian submanifolds in Kenmotsu space forms and applications
Authors: Alghamdi Fatemah Abdullah , Alqahtani Lamia Saeed , Ali Akram
Published: May 2024

In the current work, we study the geometry and topology of warped product Legendrian submanifolds in Kenmotsu space forms $\mathbb{F}^{2n+1}(\epsilon )$

F

2
n
+
1

(
ϵ
)
and derive the first Chen inequality, including extrinsic invariants such as the mean curvature and the length of the warping functions. Additionally, sectional curvature and the δ-invariant are intrinsic invariants related to this inequality. An integral bound is also given in terms of the gradient Ricci curvature for the Bochner operator formula of compact warped product submanifolds. Our primary technique is applying geometry to number structures and solving problems such as problems with Dirichlet eigenvalues.
A taxonomy of innovation spaces from the innovation networks lens
Authors: Montes Jose , Batz Aglaya , Serrano Cárdenas Lizeth Fernanda
Published: May 2024

Collaborative innovation addresses intricate, multifaceted problems of a systemic nature involving a multitude of actors with diverse and often unknown expectations. To facilitate this collaboration, innovation spaces—such as Makerspaces, Hackerspaces, Fablabs, among others—have emerged as arenas where networks of actors interconnect and solidify. Understanding the diverse nature of these innovation spaces poses a challenge, particularly in distinguishing their specific characteristics and assessing how each contributes to fostering innovative networks. This article aims to address the question: how can we classify innovation spaces based on the innovation networks they constitute? To tackle this query, we initially conducted an in-depth exploration of various innovation spaces through web content analysis, scrutinizing their individual value propositions. Subsequently, employing innovation network theory alongside domain analysis methodology, we proposed a taxonomy designed to classify the distinct types of innovation spaces under scrutiny. Our taxonomy reveals three types of spaces—learn-and-explore, partner-and impact, and transitory—as well as convergent and divergent spaces, showing the diversity and complexity of networks they constitute. The findings also show that while the majority of innovation spaces unite diverse actors to drive collaboration and innovation, many resulting networks have a medium level of formality and are project-centric. These networks tend to be synthetic in nature, seeking to apply existing knowledge, and represent exploration networks wherein the adaptability and diversity of initiatives foster learning, the acquisition of new knowledge, and the development of fresh capacities through interactions.
An empirical study of virtual museum based on dual-mode mixed visualization: the Sanxingdui bronzes
Authors: Tian Feng , Li Keying , Huang Xin , Zhang Xiaoyue , Wang Ning , Song Ying , Zhu Qiaolian , Li Yuzhi
Published: May 2024

The design and implementation of virtual museums have evolved over decades, broadening the scope of traditional museum exhibitions and serving as an effective complement to physical museums. However, virtual visual effects have consistently fallen short of replicating the authentic exhibition experience and cannot substitute for physical museums. In this paper, we report a dual-mode mixed visualization system combining computational photography and binocular stereo vision techniques, and introduce an innovative virtual museum for Sanxingdui bronzes. This approach enables visitors to engage with the artifacts in a multifaceted manner-allowing for navigation, interaction, auditory guidance, and the examination of textural details as if handling the artifacts directly. Further analysis of participant responses in terms of visual cognition and aesthetic appreciation across different settings demonstrates that this dual-mode mixed visualization delivers not only a spatiotemporal experience consistent with the real world but also surreal visual effects. This immersive experience allows audiences to experience Sanxingdui art intimately, offering both high-resolution visuals and cost-effectiveness.
The use of process data in large-scale assessments: a literature review
Authors: Anghel Ella , Khorramdel Lale , von Davier Matthias
Published: May 2024

As the use of process data in large-scale educational assessments is becoming more common, it is clear that data on examinees’ test-taking behaviors can illuminate their performance, and can have crucial ramifications concerning assessments’ validity. A thorough review of the literature in the field may inform researchers and practitioners of common findings as well as existing gaps. This literature review used topic modeling to identify themes in 221 empirical studies using process data in large-scale assessments. We identified six recurring topics: response time models, response time-general, aberrant test-taking behavior, action sequences, complex problem-solving, and digital writing. We also discuss the prominent theories used by studies in each category. Based on these findings, we suggest directions for future research applying process data from large-scale assessments.
Discrete aurora and the nightside ionosphere of Mars: an EMM–MEX conjunction of FUV imaging, ionospheric radar sounding, and suprathermal electron measurements
Authors: Harada Yuki , Fujiwara Yuka , Lillis Robert J. , Deighan Justin , Nakagawa Hiromu , Sánchez-Cano Beatriz , Lester Mark , Futaana Yoshifumi , Holmström Mats , Frahm Rudy A.
Published: May 2024

Since 2021, a new surge in discrete aurora detections at Mars has been observed by the Emirates Mars Ultraviolet Spectrometer (EMUS) onboard the Emirates Mars Mission (EMM) Hope Orbiter as EMUS started to regularly obtain synoptic auroral images with a high sensitivity. Here we report on a fortuitous conjunction between EMM and Mars Express (MEX) using far ultraviolet (FUV) imaging of discrete aurora by EMM EMUS, in situ measurements of suprathermal electrons by the MEX Analyzer of Space Plasma and Energetic Atoms Electron Spectrometer (ELS), and topside radar sounding of the nightside ionosphere by the MEX Mars Advanced Radar for Subsurface and Ionosphere Sounding (MARSIS). In this event, EMM EMUS imaged a clear discrete aurora signature around moderately strong crustal magnetic fields on the nightside near the dusk terminator, 11 min before which MEX MARSIS measured a prominent local enhancement of the peak electron density in the nightside ionosphere and MEX ELS observed an in situ enhancement of suprathermal electrons at the corresponding location. A remarkable geographic agreement is found between the enhancements of the aurora, ionosphere, and suprathermal electrons, suggesting that the enhanced ionization and auroral emission are caused concurrently by precipitating suprathermal electrons. Subsequent images indicate that the discrete aurora slightly changed its shape in 15 min and mostly disappeared in a few hours. The MEX MARSIS measurements of the auroral ionosphere display overlapping ionospheric and surface echoes indicative of horizontal gradients of the peak electron density. Analysis of the overlapping echoes implies that the auroral ionosphere and electron precipitation could be highly structured with horizontal spatial scales on the order of several tens of km. MEX MARSIS also observed a non-auroral ionospheric enhancement with a wider spatial extent than the local auroral enhancement, suggesting alternative sources of the enhanced nightside ionosphere such as plasma transport. The comparison between the ionospheric structures measured by MEX MARSIS, suprathermal electron flux measured by MEX ELS, and discrete auroral emission imaged by EMM EMUS underscores the complexity of the auroral and non-auroral nightside ionospheres. This motivates further investigations of their sources, transport, and connections to the magnetotail dynamics of Mars.
Supply chain inefficiencies: the causes and costs of dry bulk ships anchored in Vancouver
Authors: Heaver Trevor , Atkins Derek
Published: May 2024

The paper describes the contracting and logistics conditions contributing to the level of anchorage in the grain, coal and fertiliser export trades of Vancouver. Increases in the level of anchorage in these trades have created economic, environmental and governance issues. Comparison of the logistics structures and practices in these commodity trades gives insights into the reasons for different levels of anchorage. Conditions in and data from the port of Vancouver are used to simulate conditions in the grain trade and to demonstrate the critical importance of the variability of ship loading times to levels of anchorage. The incidence of the costs of anchorage is examined. The terms in voyage charterparties used in the grain trade are found to inhibit efficient use of vessel time.
Effects of low-dose magnesium oxide on elevated serum magnesium levels and improved constipation in hemodialysis patients: a single-center retrospective study
Authors: Yoshida Takuya , Furukubo Taku , Izumi Satoshi , Shoji Shigeichi , Yamakawa Tomoyuki
Published: May 2024

Background

The prevention of hypomagnesemia is critical for improving the life expectancy of patients undergoing hemodialysis (HD). The administration of magnesium oxide (MgO) increases serum Mg concentrations; however, dose adjustments must be done carefully owing to the risk of hypermagnesemia in patients undergoing HD. Additionally, the laxative effects of low-dose MgO on constipation in these patients has not been reported. Therefore, in the present study, we investigated the effects of treatment with low-dose MgO on serum Mg concentration changes and the improvement of defecation in patients undergoing HD.

Methods

The present study was a single-center retrospective observational study of 43 patients undergoing maintenance HD at Shirasagi Hospital. The patients’ clinical characteristics, including serum Mg concentration, were obtained from the hospital’s electronic medical records. In the group who received MgO for constipation, the effectiveness of MgO as a laxative was categorized as effective, ineffective, or unknown on the basis of electronic medical records related to defecation.

Results

Of the 43 patients included in the present study, 23 were prescribed supplemental Mg to prevent hypomagnesemia or maintain mild hypermagnesemia and 19 to prevent constipation. All patients received diasylate with an Mg concentration of 1.0 mEq/L. Additionally, 37 patients (86%) were treated with 330 mg/day MgO. The serum Mg concentrations before and 1, 2, and 3 months after the start of MgO were 2.3 ± 0.2, 2.8 ± 0.4, 2.8 ± 0.5, and 2.8 ± 0.4 mg/dL, respectively (P < 0.001). Multiple linear regression analysis showed that dry weight was independently associated with changes in serum Mg concentration from before to 1 month after MgO administration (regression coefficient = −0.027; P = 0.020). Of the 19 patients evaluated for constipation, 8 (42%) had documented effective bowel movements after the initiation of MgO, 6 had no effect, and 5 were unknown; however, 1 patient in the ineffective group and 2 in the effective group used other laxatives prior to starting MgO.

Conclusions

The results of the present study suggest that the administration of low-dose MgO to patients undergoing HD provides a means of increasing serum Mg concentration without causing dangerous hypermagnesemia, that serum Mg concentrations increase relatively easily in lower-weight patients. In addition, this study shows that low-dose MgO may be effective as an initial treatment for constipation in this patient group in a cautious manner, but this study did not provide sufficient data for the usefulness of low-dose MgO for constipation in patients undergoing HD.
Endovascular and percutaneous embolization of a giant post traumatic arteriovenous fistula of inferior epigastric vessels
Authors: Arunachalam Venkata Subbaih , Sharma Smily , Valakkada Jineesh , Ayyappan Anoop , Radhakrishnan Jayakrishnan , Kannath Santhosh Kumar
Published: May 2024

Background

Arteriovenous fistulas involving the anterior abdominal wall can result from trauma. Such fistulas may remain asymptomatic and undetected for a prolonged duration of time. They tend to recruit multiple arterial feeders with remodelling in the feeding arteries, making them challenging to treat.

Case presentation

We discuss a rare case of a 60-year-old male who presented with complaints of a progressive painless swelling in right lower abdomen. There was a history of blunt injury to abdomen at the same site during alleged road traffic accident 3 years ago. On CT angiography, an arteriovenous fistula was localised to the anterior abdominal wall arising predominantly from the right inferior epigastric artery with a giant venous sac and terminating as a tortuous single venous channel into the right external iliac vein. Few other small feeders were also seen arising from branches of right superior epigastric artery along Winslow’s pathway. The main challenge in endovascular management of this patient was embolization of a high flow shunt with a large venous sac and multiple arterial feeders. The dominant arterial feeder was embolized using vascular plug. The superficial location of the lesion offered an additional percutaneous window besides endovascular approach. The venous sac was percutaneously accessed and embolized using n-butyl cyanoacrylate after balloon occlusion of outflow vein. On follow up ultrasonographic evaluation at 3 months, near complete thrombosis of the venous sac was achieved.

Conclusions

Traumatic arteriovenous fistulas involving the inferior epigastric vessels are rare clinical entities. CT angiogram and digital subtraction angiography help in the optimal diagnosis and treatment planning. The use of mechanical embolization devices to cause flow arrest offers an opportunity to use liquid embolic agents which offer better percolation within the lesion. Interventional radiology offers an ideal management of these complex high flow fistulas with a good technical success and acceptable safety profile.
Typicality- and instance-dependent label noise-combating: a novel framework for simulating and combating real-world noisy labels for endoscopic polyp classification
Authors: Gao Yun , Fu Junhu , Wang Yuanyuan , Guo Yi
Published: May 2024

Learning with noisy labels aims to train neural networks with noisy labels. Current models handle instance-independent label noise (IIN) well; however, they fall short with real-world noise. In medical image classification, atypical samples frequently receive incorrect labels, rendering instance-dependent label noise (IDN) an accurate representation of real-world scenarios. However, the current IDN approaches fail to consider the typicality of samples, which hampers their ability to address real-world label noise effectively. To alleviate the issues, we introduce typicality- and instance-dependent label noise (TIDN) to simulate real-world noise and establish a TIDN-combating framework to combat label noise. Specifically, we use the sample’s distance to decision boundaries in the feature space to represent typicality. The TIDN is then generated according to typicality. We establish a TIDN-attention module to combat label noise and learn the transition matrix from latent ground truth to the observed noisy labels. A recursive algorithm that enables the network to make correct predictions with corrections from the learned transition matrix is proposed. Our experiments demonstrate that the TIDN simulates real-world noise more closely than the existing IIN and IDN. Furthermore, the TIDN-combating framework demonstrates superior classification performance when training with simulated TIDN and actual real-world noise.
Determination of global geodetic parameters using satellite laser ranging to Galileo, GLONASS, and BeiDou satellites
Authors: Li Xingxing , Lou Jiaqing , Yuan Yongqiang , Wu Jiaqi , Zhang Keke
Published: May 2024

The new Global Navigation Satellite System (GNSS) satellites, including GLONASS, Galileo, and BeiDou system, are equipped with Laser Retroreflector Arrays (LRA) to support Satellite Laser Ranging (SLR) tracking, which contributes to the estimation of global geodetic parameters. In this study, we estimate the global geodetic parameters using the SLR observations to GNSS satellites and also investigate the effects of different data processing strategies on the estimated Earth Rotation Parameters (ERP), geocenter motion, and terrestrial scale. The results indicate that setting range bias parameters for each satellite-station pair can effectively account for the satellite-specific biases induced by LRAs, leading to smaller Root Mean Square Errors (RMSE) of the post-fit SLR residuals. Furthermore, estimating the range biases for each satellite-station pair improves the accuracy of the estimated station coordinates and ERP. We also examine the impact of different arc lengths on the estimates of ERP, geocenter motion, and terrestrial scale. The results show that extending arc length can significantly reduce the formal error of ERP. The 7-day strategy produces the smallest RMSEs of 473 microarcseconds and 495 microarcseconds for the estimated X- and Y-component of pole coordinates, and 52 microseconds for length-of-day, respectively. However, the estimated geocenter motion is less affected by the arc length, even the shortest 1-day arc strategy can capture the seasonal variations of geocenter motion in Z component. For scale estimation, extending the arc length notably improves the accuracy of the estimated station coordinates and scale, but this advantage becomes less noticeable in longer arcs. The 7-day solution also obtains the closet scale results compared to ITRF2014, with the RMSE of 2.10 × 10–9.
Unusual location of pleomorphic xanthoastrocytoma: a case report
Authors: Alharbi Sara T. , Alrehaili Mona , Alhujaily Ahmed , Almashni Aysam Adnan , Almughathawi Abdulrahman
Published: May 2024

Background

Pleomorphic xanthoastrocytoma is a rare astrocytic tumor often diagnosed at a young age. Typically, they appear as supratentorial cortical tumors, frequently involving the temporal lobe with few reported rare locations. The prognosis is favorable following surgical excision; however, recurrence, dissemination, and anaplastic transformation occurred in some cases.

Case presentation

A 50-year-old female presented with convulsions and an altered consciousness. Imaging showed a periventricular mixed solid and cystic lesion. Histopathological examination revealed features of pleomorphic xanthoastrocytoma WHO grade 2 without necrosis or mitotic activity.

Conclusions

This report highlights the classic imaging findings of pleomorphic xanthoastrocytoma but in an atypical periventricular location. Although rare, pleomorphic xanthoastrocytoma should be considered in the differential diagnosis of mixed solid and cystic periventricular lesions.
Cascaded chiral birefringent media enabled planar lens with programable chromatic aberration
Authors: Zhang Dewei , Xu Chun-Ting , Chen Quan-Ming , Cao Han , Yu Hong-Guan , Tan Qing-Gui , Lu Yan-qing , Hu Wei
Published: May 2024

Wavefront control is the fundamental requirement in optical informatics. Planar optics have drawn intensive attention due to the merits of compactness and light weight. However, it remains a challenge to freely manipulate the dispersion, hindering practical applications, especially in imaging. Here, we propose the concept of frequency-synthesized phase engineering to solve this problem. A phasefront-frequency matrix is properly designed to encode different spatial phases to separate frequencies, thus makes arbitrary dispersion tailoring and even frequency-separated functionalization possible. The periodically rotated director endows cholesteric liquid crystal with a spin and frequency selective reflection. Moreover, via presetting the local initial orientation of liquid crystal, geometric phase is encoded to the reflected light. We verify the proposed strategy by cascading the chiral anisotropic optical media of specifically designed helical pitches and initial director orientations. By this means, planar lenses with RGB achromatic, enhanced chromatic aberration and color routing properties are demonstrated. Inch-sized and high-efficient lenses are fabricated with low crosstalk among colors. It releases the freedom of dispersion control of planar optics, and even enables frequency decoupled phase modulations. This work brings new insights to functional planar optics and may upgrade the performance of existing optical apparatuses.
Inclusive Research Environments for Deaf and Hard of Hearing English Speakers
Authors: Listman Jason D. , Kurz Kim B. , Picioli Amanda , Craig Paul
Published: May 2024

Need guidance on creating an inclusive lab environment for deaf and hard-of-hearing undergraduate students? This article describes the experience of deaf and hard-of-hearing students in the lab environment and lists recommendations for mentors to optimize access and gain cultural competence.
Prevalence and correlates of manic/hypomanic and depressive predominant polarity in bipolar disorder: systematic review and meta-analysis
Authors: Bartoli Francesco , Bassetti Carlo , Gazzola Marco , Gianfelice Letizia , Cavaleri Daniele , Crocamo Cristina , Carrà Giuseppe
Published: May 2024

Background

Identification of the predominant polarity, i.e. hypomanic/manic (mPP) or depressive predominant polarity (dPP), might help clinicians to improve personalised management of bipolar disorder.

Aims

We performed a systematic review and meta-analysis to estimate prevalence and correlates of mPP and dPP in bipolar disorder.

Method

The protocol was registered in the Open Science Framework Registries (https://doi.org/10.17605/OSF.IO/8S2HU). We searched main electronic databases up to December 2023 and performed random-effects meta-analyses of weighted prevalence of mPP and dPP. Odds ratios and weighted mean differences (WMDs) were used for relevant correlates.

Results

We included 28 studies, providing information on rates and/or correlates of mPP and dPP. We estimated similar rates of mPP (weighted prevalence = 30.0%, 95% CI: 23.1 to 37.4%) and dPP (weighted prevalence = 28.5%, 95% CI: 23.7 to 33.7%) in bipolar disorder. Younger age (WMD = −3.19, 95% CI: −5.30 to −1.08 years), male gender (odds ratio = 1.39, 95% CI: 1.10 to 1.76), bipolar-I disorder (odds ratio = 4.82, 95% CI: 2.27 to 10.24), psychotic features (odds ratio = 1.56, 95% CI: 1.01 to 2.41), earlier onset (WMD = −1.57, 95% CI: −2.88 to −0.26 years) and manic onset (odds ratio = 13.54, 95% CI: 5.83 to 31.46) were associated with mPP (P < 0.05). Depressive onset (odds ratio = 12.09, 95% CI: 6.38 to 22.90), number of mood episodes (WMD = 0.99, 95% CI: 0.28 to 1.70 episodes), history of suicide attempts (odds ratio = 2.09, 95% CI: 1.49 to 2.93) and being in a relationship (odds ratio = 1.98, 95% CI: 1.22 to 3.22) were associated with dPP (P < 0.05). No differences were estimated for other variables.

Conclusions

Despite some limitations, our findings support the hypothesis that predominant polarity might be a useful specifier of bipolar disorder. Evidence quality was mixed, considering effects magnitude, consistency, precision and publication bias. Different predominant polarities may identify subgroups of patients with specific clinical characteristics.
Interaction between mental disorders and social disconnectedness on mortality: a population-based cohort study
Authors: Laustsen Lisbeth Mølgaard , Ejlskov Linda , Chen Danni , Lasgaard Mathias , Gradus Jaimie L. , Østergaard Søren Dinesen , Grønkjær Marie Stjerne , Plana-Ripoll Oleguer
Published: May 2024

Background

Despite the recognised importance of mental disorders and social disconnectedness for mortality, few studies have examined their co-occurrence.

Aims

To examine the interaction between mental disorders and three distinct aspects of social disconnectedness on mortality, while taking into account sex, age and characteristics of the mental disorder.

Method

This cohort study included participants from the Danish National Health Survey in 2013 and 2017 who were followed until 2021. Survey data on social disconnectedness (loneliness, social isolation and low social support) were linked with register data on hospital-diagnosed mental disorders and mortality. Poisson regression was applied to estimate independent and joint associations with mortality, interaction contrasts and attributable proportions.

Results

A total of 162 497 individuals were followed for 886 614 person-years, and 9047 individuals (5.6%) died during follow-up. Among men, interaction between mental disorders and loneliness, social isolation and low social support, respectively, accounted for 47% (95% CI: 21–74%), 24% (95% CI: −15 to 63%) and 61% (95% CI: 35–86%) of the excess mortality after adjustment for demographics, country of birth, somatic morbidity, educational level, income and wealth. In contrast, among women, no excess mortality could be attributed to interaction. No clear trends were identified according to age or characteristics of the mental disorder.

Conclusions

Mortality among men, but not women, with a co-occurring mental disorder and social disconnectedness was substantially elevated compared with what was expected. Awareness of elevated mortality rates among socially disconnected men with mental disorders could be of importance to qualify and guide prevention efforts in psychiatric services.
Are FGFR Fusions and Mutations the Next Tumor-Agnostic Targets in Oncology?
Authors: Ellis Haley , Goyal Lipika
Published: May 2024

Gong et al present two NCI-MATCH tumor-agnostic trials evaluating erdafitinib for FGFR-altered cancers, marking steppingstones in precision oncology.
Pancreatic Neuroendocrine Tumors in French VHL Mutation Carriers
Authors: Muller Marie , Hammel Pascal , Couvelard Anne , Védie Anne-Laure , Cros Jérôme , Burnichon Nelly , Hercent Agathe , Sauvanet Alain , Richard Stéphane , de Mestier Louis
Published: May 2024

Context

Von Hippel-Lindau disease (VHL) is a rare, autosomal-dominant hereditary cancer-predisposition syndrome caused by germline pathogenic variants (PVs) in the VHL gene. It is associated with a high penetrance of benign and malignant vascular tumors in multiple organs, including pancreatic neuroendocrine tumors (PanNETs), whose long-term natural history is ill-known.

Objective

The aim of this study was to identify prognostic factors associated with VHL-related PanNETs, notably the role of genotype-phenotype correlations.

Methods

Patients with both documented germline PV in the VHL gene and PanNETs included in the French PREDIR database between 1995 and 2022 were included. The primary end point was the proportion of patients with PanNET-related metastases, and the secondary end point was overall survival (OS). Genotype/phenotype correlations were studied.

Results

We included 121 patients with 259 PanNETs. Median age at diagnosis was 38 years. Median follow-up was 89.5 months. PanNET surgical resection was performed in 51 patients. Overall, 29 patients (24%) had metastases (5 synchronous, 10 metachronous), with a higher risk in case of larger PanNET size (P = .0089; best threshold 28 mm) and grade 2 PanNET (P = .048), and a pejorative prognostic impact (P = .043). Patients with PV in VHL exon 1 had larger PanNETs (P = .018), more often metastatic disease (48% vs 11.5%; P < .001) and a trend toward shorter OS (P = .16).

Conclusion

The risk of metastases associated with VHL-related PanNETs remains low (24%) but increases with tumor size greater than 28 mm, higher grade, and in case of PV, located in VHL exon 1. These data might help improve the management of these patients, who should be referred to an expert center.