bluuebunny/crossref_metadata_2025 huggingface dataset:
Targeting STAT3 Signaling Facilitates Responsiveness of Pancreatic Cancer Cells to Chemoradiotherapy
Authors: Flebbe Hannah , Spitzner Melanie , Marquet Philipp Enno , Gaedcke Jochen , Ghadimi B. Michael , Rieken Stefan , Schneider Günter , Koenig Alexander O. , Grade Marian
Published: March 2022

The debate is ongoing regarding the potential role of preoperative chemoradiotherapy (CRT) for patients with pancreatic ductal adenocarcinoma (PDAC), and whether it should be reserved for borderline resectable or unresectable tumors. However, treatment response is heterogeneous, implicating the need to unveil and overcome the underlying mechanisms of resistance. Activation of the transcription factor STAT3 was recently linked to CRT resistance in other gastrointestinal cancers such as rectal and esophageal cancers, but its role in PDAC needs to be clarified. Protein expression and phosphorylation of STAT3 was determined in PDAC cell lines and connected to transcriptional activity measured by dual-luciferase reporter gene assays. Inhibition of STAT3 signaling was achieved by RNAi or the small-molecule inhibitor napabucasin. We observed a positive correlation between STAT3 signaling activity and CRT resistance. Importantly, genetical and pharmacological perturbation of the IL-6/STAT3 pathway resulted in CRT sensitization specifically in those cell lines, in which STAT3 activity was augmented by IL-6. In conclusion, our data underscore the general importance of IL-6/STAT3 signaling for CRT resistance and suggest that pathway inhibition may represents a putative treatment strategy in order to increase the fraction of patients with PDAC who are candidates for surgical approaches.
Functional Antagonism of Junctional Adhesion Molecule-A (JAM-A), Overexpressed in Breast Ductal Carcinoma In Situ (DCIS), Reduces HER2-Positive Tumor Progression
Authors: Smith Yvonne E. , Wang Guannan , Flynn Ciara L. , Madden Stephen F. , MacEneaney Owen , Cruz Rodrigo G. B. , Richards Cathy E. , Jahns Hanne , Brennan Marian , Cremona Mattia , Hennessy Bryan T. , Sheehan Katherine , Casucci Alexander , Sani Faizah A. , Hudson Lance , Fay Joanna , Vellanki Sri H. , O’Flaherty Siobhan , Devocelle Marc , Hill Arnold D. K. , Brennan Kieran , Sukumar Saraswati , Hopkins Ann M.
Published: March 2022

Breast ductal carcinoma in situ (DCIS) is clinically challenging, featuring high diagnosis rates and few targeted therapies. Expression/signaling from junctional adhesion molecule-A (JAM-A) has been linked to poor prognosis in invasive breast cancers, but its role in DCIS is unknown. Since progression from DCIS to invasive cancer has been linked with overexpression of the human epidermal growth factor receptor-2 (HER2), and JAM-A regulates HER2 expression, we evaluated JAM-A as a therapeutic target in DCIS. JAM-A expression was immunohistochemically assessed in patient DCIS tissues. A novel JAM-A antagonist (JBS2) was designed and tested alone/in combination with the HER2 kinase inhibitor lapatinib, using SUM-225 cells in vitro and in vivo as validated DCIS models. Murine tumors were proteomically analyzed. JAM-A expression was moderate/high in 96% of DCIS patient tissues, versus 23% of normal adjacent tissues. JBS2 bound to recombinant JAM-A, inhibiting cell viability in SUM-225 cells and a primary DCIS culture in vitro and in a chick embryo xenograft model. JBS2 reduced tumor progression in in vivo models of SUM-225 cells engrafted into mammary fat pads or directly injected into the mammary ducts of NOD-SCID mice. Preliminary proteomic analysis revealed alterations in angiogenic and apoptotic pathways. High JAM-A expression in aggressive DCIS lesions and their sensitivity to treatment by a novel JAM-A antagonist support the viability of testing JAM-A as a novel therapeutic target in DCIS.
The Impact of Radiation Dose to Heart Substructures on Major Coronary Events and Patient Survival after Chemoradiation Therapy for Esophageal Cancer
Authors: Wang Xin , Palaskas Nicolas L. , Hobbs Brian P. , Abe Jun-ichi , Nead Kevin T. , Yusuf Syed Wamique , Hermann Joerg , Deswal Anita , Lin Steven H.
Published: March 2022

Background: There is a paucity of data regarding the association between radiation exposure of heart substructures and the incidence of major coronary events (MCEs) in patients with esophageal cancer (ESOC) undergoing chemoradiation therapy. We studied radiation dosimetric determinants of MCE risk and measured their impact on patient prognosis using a cohort of ESOC patients treated at a single institution. Methods: Between March 2005 and October 2015, 355 ESOC patients treated with concurrent chemoradiotherapy were identified from a prospectively maintained and institutional-regulatory-board-approved clinical database. Dose-distribution parameters of the whole heart, the atria, the ventricles, the left main coronary artery, and three main coronary arteries were extracted for analysis. Results: Within a median follow-up time of 67 months, 14 patients experienced MCEs at a median of 16 months. The incidence of MCEs was significantly associated with the left anterior descending coronary artery (LAD) receiving ≥30 Gy (V30Gy) (p = 0.048). Patients receiving LAD V30Gy ≥ 10% of volume experienced a higher incidence of MCEs versus the LAD V30Gy < 10% group (p = 0.044). The relative rate of death increased with the left main coronary artery (LMA) mean dose (Gy) (p = 0.002). Furthermore, a mutual promotion effect of hyperlipidemia and RT on MCEs was observed. Conclusion: Radiation dose to coronary substructures is associated with MCEs and overall survival in patients with ESOC. In this study, the doses to these substructures appeared to be better predictors of toxicity outcomes than mean heart dose (MHD) or whole-heart V30Gy. These findings have implications for reducing coronary events through radiation therapy planning.
Microwave Ablation for Colorectal Liver Metastases: A Systematic Review and Pooled Oncological Analyses
Authors: Mimmo Antonio , Pegoraro Francesca , Rhaiem Rami , Montalti Roberto , Donadieu Alix , Tashkandi Ahmad , Al-Sadairi Abdul Rahman , Kianmanesh Reza , Piardi Tullio
Published: March 2022

(1) Background: colorectal liver metastases (CRLM) are the most common extra-lymphatic metastases in colorectal cancer; however, few patients are fit for curative surgery. Microwave ablation (MWA) showed promising outcomes in this cohort of patients. This systematic review and pooled analysis aimed to analyze the oncological results of MWA for CRLM. (2) Methods: Following PRISMA guidelines, PubMed, Scopus, EMBASE, Google Scholar, Science Direct, and the Wiley Online Library databases were searched for reports published before January 2021. We included papers assessing MWA, treating resectable CRLM with curative intention. We evaluated the reported MWA-related complications and oncological outcomes as being recurrence-free (RF), free from local recurrence (FFLR), and overall survival rates (OS). (3) Results: Twelve out of 4822 papers (395 patients) were finally included. Global RF rates at 1, 3, and 5 years were 65.1%, 44.6%, and 34.3%, respectively. Global FFLR rates at 3, 6, and 12 months were 96.3%, 89.6%, and 83.7%, respectively. Global OS at 1, 3, and 5 years were 86.7%, 59.6%, and 44.8%, respectively. A better FFLR was reached using the MWA surgical approach at 3, 6, and 12 months, with reported rates of 97.1%, 92.7%, and 88.6%, respectively. (4) Conclusions: Surgical MWA treatment for CRLM smaller than 3 cm is a safe and valid option. This approach can be safely included for selected patients in the curative intent approaches to treating CRLM.
Activation of Investigator-Initiated Clinical Trials with a Pharmaceutical for Cancer Patients before and after Post-Millennial Changes of Regulations in Germany and Europe
Authors: Berdel Wolfgang E.
Published: March 2022

Shortly after the beginning of the year 2000, multiple legal changes with impacts on the regulatory framework of clinical trials became effective almost simultaneously. They included the European Union (EU) Clinical Trial Directive (CTD) 2001/20 followed by major changes in national drug laws, the change in the legal status of German University Hospitals (1998), and a new disease-related groups (DRG)-based reimbursement system for hospitals in Germany (2000). Together, these changes created enormous bureaucratic and financial inhibition of activation and conduct of academic investigator-initiated clinical trials (IIT). Examples for activating clinical trials in oncology before and after 2004 are outlined and discussed, focussing on extended time frames, the establishment of centralized responsibility structures and the exploding financial consequences. In addition, the evolution of trial numbers and the distribution of trial initiators between “commercial” and “academic” over time are discussed together with the occurrence of clinical registries. At the same time, progress in molecular biology led to a plethora of new targets for effective pharmacological therapy of life-threatening diseases such as cancer, and the overall number of clinical trials has not decreased. Yet, judging the regulatory and administrative hurdles between scientific study design and first-patient on trial before and after 2004 and weighing these against the lack of evidence that this regulation has achieved its goal to enhance patient safety and trial quality, the necessity to completely overhaul this CTD becomes obvious. A main goal of such an initiative should be to minimize bureaucracy. For the specific situation in Germany, relocation of responsibility and freedom to operate in University Hospitals and Medical Faculties back to the physician–scientists and reduction in interference by legal divisions should be a goal as well as increasing the public financial support for IITs.
Transcending Blood—Opportunities for Alternate Liquid Biopsies in Oncology
Authors: Werner Bonnita , Warton Kristina , Ford Caroline E.
Published: March 2022

Cell-free DNA (cfDNA) is a useful molecular biomarker in oncology research and treatment, but while research into its properties in blood has flourished, there remains much to be discovered about cfDNA in other body fluids. The cfDNA from saliva, sputum, cerebrospinal fluid, urine, faeces, pleural effusions, and ascites has unique advantages over blood, and has potential as an alternative ‘liquid biopsy’ template. This review summarises the state of current knowledge and identifies the gaps in our understanding of non-blood liquid biopsies; where their advantages lie, where caution is needed, where they might fit clinically, and where research should focus in order to accelerate clinical implementation. An emphasis is placed on ascites and pleural effusions, being pathological fluids directly associated with cancer. We conclude that non-blood fluids are viable sources of cfDNA in situations where solid tissue biopsies are inaccessible, or only accessible from dated archived specimens. In addition, we show that due to the abundance of cfDNA in non-blood fluids, they can outperform blood in many circumstances. We demonstrate multiple instances in which DNA from various sources can provide additional information, and thus we advocate for analysing non-blood sources as a complement to blood and/or tissue. Further research into these fluids will highlight opportunities to improve patient outcomes across cancer types.
Influence of Plasma Surface Treatment of Polyimide on the Microstructure of Aluminum Thin Films
Authors: Kondoh Eiichi
Published: March 2022

The effects of plasma treatment of polyimide substrates on the texture and grain size distribution of aluminum thin films were studied. Oxygen-argon plasma treatment increased the average grain size and enhanced the (111) film texture. For short oxygen-argon plasma treatment times, the deposited Al films showed a (111) texture with a bimodal grain structure and even a {111}<112¯> type in-plane texture. The preferential nucleation and grain growth of (111) grains are discussed in terms of the interface energy anisotropy.
Multimodal Imaging and Biomarkers in Cardiac Amyloidosis
Authors: Jung Mi-Hyang , Chang Suyon , Han Eun Ji , Youn Jong-Chan
Published: March 2022

Amyloidosis is a progressive infiltrative disease instigated by the extracellular deposition of amyloid fibrils in various organs such as the heart, kidney, and peripheral nerves. Cardiac amyloid deposits cause restrictive cardiomyopathy, leading to a poor prognosis in systemic amyloidosis. The most common etiologies of cardiac amyloidosis (CA) are immunoglobulin light chain deposits (AL-CA) and misfolded transthyretin deposits (ATTR-CA). In recent years, many developments have been accomplished in the field of diagnosis and treatment of CA. At present, ATTR-CA can be noninvasively diagnosed if the following two conditions are fulfilled in the setting of typical echocardiographic/cardiac MRI findings: (1) grade 2 or 3 myocardial uptake in bone scintigraphy confirmed by SPECT and (2) absence of monoclonal protein confirmed by serum-free light chain assay, and serum/urine protein electrophoresis with immunofixation test. Effective therapies are evolving in both types of CA (tafamidis for ATTR-CA and immunologic treatments for AL-CA). Thus, early suspicion and prompt diagnosis are crucial for achieving better outcomes. In this review, we have summarized the role of multimodal imaging (e.g., echocardiography, cardiac MRI, and bone scintigraphy) and biomarkers (e.g., troponin, BNP) in the diagnosis, risk stratification, and treatment monitoring of CA.
The Importance of Networking: Plant Polycomb Repressive Complex 2 and Its Interactors
Authors: Godwin James , Farrona Sara
Published: March 2022

Polycomb Repressive Complex 2 (PRC2) is arguably the best-known plant complex of the Polycomb Group (PcG) pathway, formed by a group of proteins that epigenetically represses gene expression. PRC2-mediated deposition of H3K27me3 has amply been studied in Arabidopsis and, more recently, data from other plant model species has also been published, allowing for an increasing knowledge of PRC2 activities and target genes. How PRC2 molecular functions are regulated and how PRC2 is recruited to discrete chromatin regions are questions that have brought more attention in recent years. A mechanism to modulate PRC2-mediated activity is through its interaction with other protein partners or accessory proteins. Current evidence for PRC2 interactors has demonstrated the complexity of its protein network and how far we are from fully understanding the impact of these interactions on the activities of PRC2 core subunits and on the formation of new PRC2 versions. This review presents a list of PRC2 interactors, emphasizing their mechanistic action upon PRC2 functions and their effects on transcriptional regulation.
Importance of Dead Wood in Virgin Forest Ecosystem Functioning in Southern Carpathians
Authors: Chivulescu Șerban , Pitar Diana , Apostol Bogdan , Leca Ștefan , Badea Ovidiu
Published: March 2022

Dead wood (DW) is an important component of sustainable forest management and climate change mitigation. Three research plots (each with an area of 1 ha), located in virgin forests in the Southern Carpathians (Semenic P20, Retezat–Zănoaga, and Făgăraș–Șinca), were installed in order to study the synergies between DW and climate change mitigation effects. Data on the dendrometric characteristics of standing and lying DW, the species, and the degree of decay were recorded. The aboveground biomass (AGB) and carbon stock (CS) of the DW were also determined. The DW volume was between 48 m3·ha−1 and 148 m3·ha−1, with the total volume (dead and alive) ranging between 725 m3·ha−1 and 966 m3·ha−1. The DW volume distribution shows a decreasing trend, with the most suitable theoretical distributions for describing this being the lognormal, the 2P Weibull, and the 2P-Gamma. The AGB ranged between 17 t·ha−1 and 30 t·ha−1 and showed a decreasing trend according to altitude. The CS was between 8 t·ha−1 and 14.33 t·ha−1. A slow decomposition rate for the hardwood was identified by analyzing the relationship between the surface and volume of the DW. This highlighted the capacity of DW to store carbon for a long period of time.
Detecting Coastal Wetland Degradation by Combining Remote Sensing and Hydrologic Modeling
Authors: He Keqi , Zhang Yu , Li Wenhong , Sun Ge , McNulty Steve
Published: March 2022

Sea-level rise and climate change stresses pose increasing threats to coastal wetlands that are vital to wildlife habitats, carbon sequestration, water supply, and other ecosystem services with global significance. However, existing studies are limited in individual sites, and large-scale mapping of coastal wetland degradation patterns over a long period is rare. Our study developed a new framework to detect spatial and temporal patterns of coastal wetland degradation by analyzing fine-scale, long-term remotely sensed Normalized Difference Vegetation Index (NDVI) data. Then, this framework was tested to track the degradation of coastal wetlands at the Alligator River National Wildlife Refuge (ARNWR) in North Carolina, United States, during the period from 1995 to 2019. We identified six types of coastal wetland degradation in the study area. Most of the detected degradation was located within 2 km from the shoreline and occurred in the past five years. Further, we used a state-of-the-art coastal hydrologic model, PIHM-Wetland, to investigate key hydrologic processes/variables that control the coastal wetland degradation. The temporal and spatial distributions of simulated coastal flooding and saltwater intrusion confirmed the location and timing of wetland degradation detected by remote sensing. The combined method also quantified the possible critical thresholds of water tables for wetland degradation. The remote sensing–hydrologic model integrated scheme proposed in this study provides a new tool for detecting and understanding coastal wetland degradation mechanisms. Our study approach can also be extended to other coastal wetland regions to understand how climate change and sea-level rise impact wetland transformations.
Supplementation of Oilseeds to an Herbage Diet High in Condensed Tannins Affects Methane Production with Minimal Impact on Ruminal Fermentation in Continuous Culture
Authors: Billman Eric D. , Dillard S. Leanne , Roca-Fernández Ana Isabel , Soder Kathy J.
Published: March 2022

Condensed tannins (CT) have been observed to reduce enteric CH4 production when added to ruminant diets. However, high concentrations of CT in forages such as sericea lespedeza (SL; Lespedeza cuneata (Dum. Cours.) G. Don) may depress nutrient digestibility. Oilseed crops, high in lipid concentration, also reduce enteric CH4 via toxicity to methanogenic bacteria with less depression of nutrient digestibility. However, it is unclear whether combining these two feeds would result in even greater decreases in CH4 without impairing ruminal fermentation. This study used an in vitro continuous culture fermentor system to determine if supplementation of ground oilseeds would further reduce enteric CH4 production while improving nutrient digestibility of high-CT forages. The experimental design was a 4 × 4 Latin square, with four diets containing (dry matter basis) 45% orchardgrass (OCH; Dactylis glomerata L.), 45% sericea lespedeza (SL; Lespedeza cuneata (Dum. Cours.) G. Don), and 10% oilseed supplements, using canola (CAN; Brassica napus L.), soybean (SOY; Glycine max L.), sunflower (SUN; Helianthus annuus L.), or a mix of all three species (MIX; in equal proportions). Fermentors were fed 82 g of dry matter/d in four equal feedings over four 10 d periods. Methane was recorded every 10 min, and effluent samples were analyzed for pH, volatile fatty acids, dry matter, organic matter, crude protein, neutral detergent fiber, and acid detergent fiber to determine apparent and true nutrient digestibilities. The CAN, SUN, and MIX diets had greater concentrations of crude fat (7–8 g/kg) than the SOY diet (5.7 g/kg), which contributed to the greater reduction in enteric CH4 production in those diets (13–27 mg/d) compared to the SOY diet (84 mg/d). Apparent and true nutrient digestibilities were not affected by the addition of ground oilseeds. While N intake increased concomitant with crude protein increases in the diets, there were no additional effects on N flows. While supplementing a high-CT diet with any of the three oilseeds (canola, soybean, sunflower, or a mixture of the three oilseeds) reduced total CH4 emission without depressing nutrient digestibility, canola and mixes containing canola were most effective. Further research is needed in vivo to evaluate whether these results translate to greater feed efficiency and animal production.
A Note on a Coupled System of Hilfer Fractional Differential Inclusions
Authors: Cernea Aurelian
Published: March 2022

A coupled system of Hilfer fractional differential inclusions with nonlocal integral boundary conditions is considered. An existence result is established when the set-valued maps have non-convex values. We treat the case when the set-valued maps are Lipschitz in the state variables and we avoid the applications of fixed point theorems as usual. An illustration of the results is given by a suitable example.
Genetic Predisposition to Schizophrenia and Depressive Disorder Comorbidity
Authors: Shnayder Natalia A. , Novitsky Maxim A. , Neznanov Nikolay G. , Limankin Oleg V. , Asadullin Azat R. , Petrov Artem V. , Dmitrenko Diana V. , Narodova Ekaterina A. , Popenko Natalia V. , Nasyrova Regina F.
Published: March 2022

Background: Patients with schizophrenia have an increased risk of depressive disorders compared to the general population. The comorbidity between schizophrenia and depression suggests a potential coincidence of the pathophysiology and/or genetic predictors of these mental disorders. The aim of this study was to review the potential genetic predictors of schizophrenia and depression comorbidity. Materials and Methods: We carried out research and analysis of publications in the databases PubMed, Springer, Wiley Online Library, Taylor & Francis Online, Science Direct, and eLIBRARY.RU using keywords and their combinations. The search depth was the last 10 years (2010–2020). Full-text original articles, reviews, meta-analyses, and clinical observations were analyzed. A total of 459 articles were found, of which 45 articles corresponding to the purpose of this study were analyzed in this topic review. Results: Overlap in the symptoms and genetic predictors between these disorders suggests that a common etiological mechanism may underlie the presentation of comorbid depression in schizophrenia. The molecular mechanisms linking schizophrenia and depression are polygenic. The most studied candidate genes are GRIN1, GPM6A, SEPTIN4, TPH1, TPH2, CACNA1C, CACNB2, and BCL9. Conclusion: Planning and conducting genome-wide and associative genetic studies of the comorbid conditions under consideration in psychiatry is important for the development of biological and clinical predictors and a personalized therapy strategy for schizophrenia. However, it should be recognized that the problems of predictive and personalized psychiatry in the diagnosis and treatment of schizophrenia and comorbid disorders are far from being resolved.
An Experimental Study on the Effect of Temperature on the Shear Strength Behavior of a Silty Clay Soil
Authors: Hailemariam Henok , Wuttke Frank
Published: March 2022

In this paper, we report on the results of an experimental study of the yielding and shear strength behavior of a normally consolidated silty clay soil from eastern Germany. The shear strength tests were performed on remolded forms of the soil using a triaxial cell which can regulate the temperature of the specimen. The experimental program comprised a series of pressure stepping compression consolidated drained (CD) triaxial tests at temperatures of 20, 40 and 60 °C. Overall, although the volume change behavior of the soil was found to exhibit some variations with changes in temperature, possibly due to changes in the double layer thickness of the clay fraction of the soil upon heating, the shear strength behavior (in terms of effective angle of internal friction) of the silty clay soil was found to exhibit minor changes with variations in temperature and can be assumed to be independent of temperature for the range considered in this study.
Visible Light-Based Ag3PO4/g-C3N4@MoS2 for Highly Efficient Degradation of 2-Amino-4-acetylaminoanisole (AMA) from Printing and Dyeing Wastewater
Authors: Liu Hong , Chen Houwang , Ding Ning
Published: March 2022

In this research, the preparation of a Ag3PO4/g-C3N4@MoS2 photocatalyst and the performance and mechanism of degradation of 2-amino-4-acetaminoanisole (AMA) were studied. The phase composition and morphology of the synthesized samples were comprehensively characterized by X-ray diffraction (XRD), scanning electron microscope (SEM), ultraviolet–visible diffuse reflectance (UV–Vis), and photoelectron spectroscopy (XPS). The catalytic performance of the photocatalyst was evaluated by the visible-light catalytic degradation of the AMA. The experimental results show that the Ag3PO4/g-C3N4@MoS2 composite photocatalyst has stronger photocatalytic oxidation and reduction capabilities than Ag3PO4 and Ag3PO4/g-C3N4. The material only decreases by 31.3% after five cycles of use, indicating that the material has good light stability. Free radical capture experiments prove that photo-generated holes (h+) and superoxide radicals (·O2−) are the main active substances in the photocatalytic process. The fundamental studies in the present research provide a new perspective for constructing an innovative type of visible-light photocatalyst and a new way to promote the photocatalytic degradation of organic pollutants.
Identification of Candidate lncRNA and Pseudogene Biomarkers Associated with Carbon-Nanotube-Induced Malignant Transformation of Lung Cells and Prediction of Potential Preventive Drugs
Authors: Chang Guangtao , Xie Dongli , Hu Jianchen , Wu Tong , Cao Kangli , Luo Xiaogang
Published: March 2022

Mounting evidence has linked carbon nanotube (CNT) exposure with malignant transformation of lungs. Long non-coding RNAs (lncRNAs) and pseudogenes are important regulators to mediate the pathogenesis of diseases, representing potential biomarkers for surveillance of lung carcinogenesis in workers exposed to CNTs and possible targets to develop preventive strategies. The aim of this study was to screen crucial lncRNAs and pseudogenes and predict preventive drugs. GSE41178 (small airway epithelial cells exposed to single- or multi-walled CNTs or dispersant control) and GSE56104 (lung epithelial cells exposed to single-walled CNTs or dispersant control) datasets were downloaded from the Gene Expression Omnibus database. Weighted correlation network analysis was performed for these two datasets, and the turquoise module was preserved and associated with CNT-induced malignant phenotypes. In total, 24 lncRNAs and 112 pseudogenes in this module were identified as differentially expressed in CNT-exposed cells compared with controls. Four lncRNAs (MEG3, ARHGAP5-AS1, LINC00174 and PVT1) and five pseudogenes (MT1JP, MT1L, RPL23AP64, ZNF826P and TMEM198B) were predicted to function by competing endogenous RNA (MEG3/RPL23AP64-hsa-miR-942-5p-CPEB2/PHF21A/BAMBI; ZNF826P-hsa-miR-23a-3p-SYNGAP1, TMEM198B-hsa-miR-15b-5p-SYNGAP1/CLU; PVT1-hsa-miR-423-5p-PSME3) or co-expression (MEG3/MT1L/ZNF826P/MT1JP-ATM; ARHGAP5-AS1-TMED10, LINC00174-NEDD4L, ARHGAP5-AS1/PVT1-NIP7; MT1L/MT1JP-SYNGAP1; MT1L/MT1JP-CLU) mechanisms. The expression levels and prognosis of all genes in the above interaction pairs were validated using lung cancer patient samples. The receiver operating characteristic curve analysis showed the combination of four lncRNAs, five pseudogenes or lncRNAs + pseudogenes were all effective for predicting lung cancer (accuracy >0.8). The comparative toxicogenomics database suggested schizandrin A, folic acid, zinc or gamma-linolenic acid may be preventive drugs by reversing the expression levels of lncRNAs or pseudogenes. In conclusion, this study highlights lncRNAs and pseudogenes as candidate diagnostic biomarkers and drug targets for CNT-induced lung cancer.
“She’ll Be Right, Mate”: A Mixed Methods Analysis of Skin Cancer Prevention Practices among Australian Farmers—An At-Risk Group
Authors: Trenerry Camilla , Fletcher Chloe , Wilson Carlene , Gunn Kate
Published: March 2022

This study examined Australian farmers’ engagement with skin cancer prevention behaviours and explored what made it hard for them to be ‘SunSmart’ (barriers), and what could be done to make prevention easier (facilitators). In total, 498 farmers (83.1% male, 22–89 years, 50.8% grain, sheep, or cattle farmers) participated. The least frequently performed SunSmart behaviours (reported as never practiced during summer) were using SPF 30+ sunscreen (16.6%), wearing protective sunglasses (10.5%), and wearing protective clothing (8.6%). Greater engagement (i.e., higher scores on scale from Never to Always) with SunSmart behaviours was explained by gender (female), educational attainment (trade or technical college certificate vs. high school), personal skin cancer history, and skin sun sensitivity. Barriers reported by farmers related to personal preferences (e.g., short-sleeved rather than long-sleeved clothing), comfort, and perceived impracticality of sun protection. Farmers’ solutions included making protective clothing and sunscreen more appropriate for farm work (e.g., by making clothing more breathable). A personal health scare was the most reported motivation for skin cancer prevention. Findings highlight the need for increased access to sun-protective clothing and sunscreen that is suitable for wearing when working on farms, complemented by culturally appropriate health education messaging, to encourage more farmers to perform SunSmart behaviours.
Innovative Strategies to Fuel Organic Food Business Growth: A Qualitative Research
Authors: Chen Sonia Chien-I , Liu Chenglian , Wang Zhenyuan , Arya Farid
Published: March 2022

This study aimed to identify the factors affecting consumer behavior and customer loyalty toward organic food. Whether consumers seek organic food for a healthy body or more as food for thought continues to be debated. However, since consumers’ purchase habits are based on their honest life experiences, which shape the building of a brand, this study reviewed the extant literature to understand the factors influencing the purchasing behavior for organic food. The follow-up problems highlighted in the research are related to organic business marketing strategy. Based on our methodology, we conducted semi-structured interviews to gain themes for qualitative research. The study found that availability, variety, and taste were the top three factors affecting consumers’ purchase decisions; surprisingly, neither price nor health-consciousness was the first concern. Using market-led innovation as an innovative lens to understand customer loyalty, this research highlights sustainable and advantageous business practices in the organic food market to enrich the literature on organic food purchasing behavior from multiple stakeholders.
Does Guided Written Disclosure Reduce Distress and Improve Psychological Functioning in Patients with Skin Diseases?
Authors: Quinto Rossella Mattea , Iani Luca , De Vincenzo Francesco , Russo Francesca , Porcelli Piero , Abeni Damiano
Published: March 2022

Background. Skin diseases (e.g., psoriasis and systemic sclerosis) are generally associated with negative psychosocial outcomes. Although different psychological interventions have been used to improve the quality of life of dermatological patients, the effects of the guided written disclosure (GWD) protocol have not been previously examined in these patients. Moreover, little attention has been paid to positive psychology constructs. Methods. This study investigates the effectiveness of GWD on positive and negative functioning in dermatological patients. Pre- and 1-month post-intervention measures included emotion regulation, sense of inner peace, skin-related symptoms and functioning, sense of coherence, and psychological distress. Results. A total of 196 consecutive outpatients were randomly assigned to GWD and active control groups, of whom 60 (30.6%) completed the study and 45 (GWD: n = 24; AC: n = 21) provided complete data. Our results did not show any significant difference between the experimental and control groups in the outcome variables, whereas non-completers reported higher levels of distress, unpleasant skin-related emotions, and lower cognitive reappraisal compared to completers. Conclusions. These findings show a poor compliance, and suggest that expressive writing is not well accepted by patients and is not effective in improving positive and negative psychological functioning in dermatological patients.
Choosing between Homologous or Heterologous COVID-19 Vaccination Regimens: A Cross-Sectional Study among the General Population in Italy
Authors: Clari Marco , Godono Alessandro , Albanesi Beatrice , Casabona Elena , Comoretto Rosanna Irene , Mansour Ihab , Conti Alessio , Dimonte Valerio , Ciocan Catalina
Published: March 2022

A shortage of COVID-19 vaccines and reports of side-effects led several countries to recommend a heterologous regimen for second vaccine doses. This study aimed to describe the reasons behind individuals’ choices of a homologous or a heterologous second vaccination. This cross-sectional study enrolled individuals under 60 who had received a first dose of Vaxzevria and could choose between a homologous or heterologous regimen for their second dose. Quantitative (socio-demographic, clinical characteristics) and qualitative data were collected and analysed through a generalized linear model and thematic analysis, respectively. Of the 1437 individuals included in the analysis, the majority (76.1%) chose a heterologous second dose of the COVID-19 vaccination. More females chose a heterologous vaccination regimen (p = 0.003). Younger individuals also tended to choose heterologous vaccination (p < 0.001). The main motivation in favour of heterologous vaccination was to follow the Italian Ministry of Health recommendations (n = 118; 53.9%). This study showed that most individuals, mainly younger people and females, chose a heterologous dose of COVID-19 vaccination after their first viral vector vaccine. Heterologous vaccinations could be an effective public health measure to control the pandemic as they are a safe and efficient alternative to homologous regimens.
The Impact of the COVID-19 Outbreak on Mental Wellbeing in Children with a Chronic Condition Compared to Healthy Peers
Authors: Hoefnagels Johanna W. , Schoen Annelieke B. , van der Laan Sabine E. I. , Rodijk Lyan H. , van der Ent Cornelis K. , van de Putte Elise M. , Dalmeijer Geertje W. , Nijhof Sanne L.
Published: March 2022

The aim of this study was to assess the impact of the COVID-19 pandemic on the mental wellbeing of children 8–18 years old with chronic conditions, by comparing pandemic data with pre-pandemic data and with healthy peers. Data were obtained from two ongoing longitudinal cohorts: the PROactive cohort study following children with a chronic condition, and the WHISTLER population cohort. Mental wellbeing was assessed by three indicators: life satisfaction, internalising symptoms, and psychosomatic health. The stringency of the COVID-19-related lockdown was considered a moderating factor. Data on chronic patients were recorded before (n = 934, 65% girls) and during (n = 503, 61% girls) the pandemic, and compared to healthy peers during the pandemic (n = 166, 61% girls). Children with a chronic condition reported lower life satisfaction, but no clinically relevant changes in internalising symptoms or psychosomatic health, during the pandemic compared to before. In comparison to healthy peers, children with a chronic condition experienced decreased life satisfaction and psychosomatic health, but internalising symptoms did not differ between groups during the COVID-19 pandemic. The lockdown stringency was negatively associated with all indicators of mental wellbeing—worse life satisfaction, more internalising symptoms, and more psychosomatic symptoms.
Computational Study of Thermal Comfort and Reduction of CO2 Levels inside a Classroom
Authors: Ovando-Chacon Guillermo Efren , Rodríguez-León Abelardo , Ovando-Chacon Sandy Luz , Hernández-Ordoñez Martín , Díaz-González Mario , Pozos-Texon Felipe de Jesús
Published: March 2022

Due to the current COVID-19 pandemic, guaranteeing thermal comfort and low CO2 levels in classrooms through efficient ventilation has become vitally important. This study presents three-dimensional simulations based on computational fluid dynamics of airflow inside an air-conditioned classroom located in Veracruz, Mexico. The analysis included various positions of an air extractor, Reynolds numbers up to 3.5 × 104, four different concentrations of pollutant sources, and three different times of the day. The simulations produced velocity, air temperature, and CO2 concentrations fields, and we calculated average air temperatures, average CO2 concentrations, and overall ventilation effectiveness. Our results revealed an optimal extractor position and Reynolds number conducive to thermal comfort and low CO2 levels due to an adequate ventilation configuration. At high pollutant concentrations, it is necessary to reduce the number of students in the classroom to achieve safe CO2 levels.
Conventional Event Tree Analysis on Emergency Release of Liquefied Natural Gas
Authors: Zwęgliński Tomasz
Published: March 2022

Liquefied natural gas (LNG) is stored in facilities located in urban areas and transported over public roads. A shift towards the broader use of LNG is economically and environmentally justified. However, it triggers an obvious need for an investigation of LNG risk through elaboration and validation of potential scenarios that the hazard might generate if it materialises. This background knowledge and past experiences were elaborated in the course of a literature review and field experiments towards designing the conventional event trees on LNG emergency release for three different units, such as a storage tank, a pipeline and in road or railway transportation. The research allowed us to answer the following question: what are the key scenario lines LNG incidents might follow? Thus, it constitutes a valuable tool for designing, planning, organising, executing and evaluating trainings and exercises on LNG emergencies.
Bereavement Needs Assessment in Nurses: Elaboration and Content Validation of a Professional Traumatic Grief Scale
Authors: Gilart Ester , Lepiani Isabel , Dueñas María , Cantizano Nuñez Maria José , Baena Belen Gutierrez , Bocchino Anna
Published: March 2022

The COVID-19 pandemic has caused a series of biopsychosocial repercussions among nursing professionals. The impossibility of anticipating the events, the numerous deaths, the excessive workload, the lack of personal health and the necessary means of protection made it difficult to regulate the impact and the elaboration of grief to the point of becoming, on many occasions, a traumatic grief whose physical and psychological manifestations are becoming more and more evident. The main objective of this research was to develop a scale for a group of symptoms based on professional traumatic grief. The development consisted of two phases: (I) instrument design through a literature review and focus groups of bereavement experts and healthcare professionals who experience the grief process in their work; and (II) validation of the content of the instrument. A total of 25 final items were established as suitable for inclusion in the instrument. It is expected that the experiences and results obtained through the development and validation of a scale of specific symptomatology of professional traumatic grief in health professionals will allow the assessment and detection of symptomatology in order to develop programs and strategies for early intervention and prevention.
Vaccine Hesitancy and the Green Digital Pass: A Study on Adherence to the Italian COVID-19 Vaccination Campaign
Authors: Moccia Giuseppina , Carpinelli Luna , Savarese Giulia , De Caro Francesco
Published: March 2022

Background: In July 2021, the vaccination campaign in Italy suffered a sudden setback, and the number of vaccine administrations decreased dramatically. On 20 July 2021, the obligation of the Green Digital Pass came into force in order to access work and leisure places, penalizing those who had not been vaccinated. The purpose of this work was to investigate the phenomenon of vaccination hesitancy and the underlying reasons, as well as any changes to the membership following the obligation of the Green Pass. Methods: A total of 83 subjects (45.8% F; mean age 22.24 ± 4.308) participated in the survey during the post-vaccine observation phase at the Vaccinal Center of the University Hospital “San Giovanni di Dio e Ruggi d’Aragona” (Salerno, Italy). The questionnaire collected anamnestic information, as well as data on state anxiety (STAI-Y), perception of quality of life (SF-12), perception of COVID-19 risks, and vaccine hesitancy. Results: Among participants, 19.3% reported hesitation. The most common concerns about the COVID-19 vaccine concerned safety and efficacy (4.9%) and the obligation of the Green Pass (4.9%). Conclusions: Findings suggest that delving into the phenomenon of vaccine hesitancy can help to enhance vaccination strategies in order to gain widespread acceptance, a key path to ensuring a quick way out of the current pandemic emergency.
Efficient Production of Naringin Acetate with Different Acyl Donors via Enzymatic Transesterification by Lipases
Authors: Baek Yesol , Lee Seungmee , Son Jemin , Lee Taek , Oh Jong-Min , Lee Sang Hun , Kim Hyun Uk , Seo Sang Woo , Park Si Jae , Yoo Hah Young , Park Chulhwan
Published: March 2022

Naringin, one of the citrus flavonoids and known as a natural antioxidant, has limited bioavailability owing to its low stability and solubility. However, naringin esters formed via acylation have recently been reported to possess improved physical and chemical properties. The development of these compounds has a great potential in the food, cosmetic and pharmaceutical industries, but low conversion and productivity are barriers to industrial applications. This study aimed to improve the conversion of naringin acetate, which is formed via the enzymatic reaction between naringin and an acyl donor. An optimal reaction condition was determined by evaluating the effect of various variables (enzyme type, enzyme concentration, acyl donor, molar ratio of reactants, reaction temperature, and solvent) on the synthesis of naringin acetate. The optimal condition was as follows: 3 g/L of Lipozyme TL IM, molar ratio of 1:5 (naringin:acyl donor), reaction temperature of 40 °C, and acetonitrile as the reaction solvent. Under this condition, the maximum conversion to naringin acetate from acetic anhydride and vinyl acetate was achieved at approximately 98.5% (8 h) and 97.5% (24 h), respectively. Compared to the previously reported values, a high conversion was achieved within a short time, confirming the commercial potential of the process.
Exclusive Breastfeeding and Vitamin D Supplementation: A Positive Synergistic Effect on Prevention of Childhood Infections?
Authors: Domenici Raffaele , Vierucci Francesco
Published: March 2022

Human milk is the best food for infants. Breastfeeding has been associated with a reduced risk of viral and bacterial infections. Breast milk contains the perfect amount of nutrients needed to promote infant growth, except for vitamin D. Vitamin D is crucial for calcium metabolism and bone health, and it also has extra-skeletal actions, involving innate and adaptive immunity. As exclusive breastfeeding is a risk factor for vitamin D deficiency, infants should be supplemented with vitamin D at least during the first year. The promotion of breastfeeding and vitamin D supplementation represents an important objective of public health.
Substance and Behavioral Addictions among American Indian and Alaska Native Populations
Authors: Soto Claradina , West Amy E. , Ramos Guadalupe G. , Unger Jennifer B.
Published: March 2022

Objective: This paper examines substance and behavioral addictions among American Indian and Alaska Natives (AIAN) to identify the structural and psychosocial risk and cultural protective factors that are associated with substance use and behavioral addictions. Methods: Five databases were used to search for peer reviewed articles through December 2021 that examined substance and behavioral addictions among AIANs. Results: The literature search identified 69 articles. Numerous risk factors (i.e., life stressors, severe trauma, family history of alcohol use) and protective factors (i.e., ethnic identity, family support) influence multiple substance (i.e., commercial tobacco, alcohol, opioid, stimulants) and behavioral (e.g., gambling) addictions. Conclusions: There is a dearth of research on behavioral addictions among AIANs. Unique risk factors in AIAN communities such as historical trauma and socioeconomic challenges have interfered with traditional cultural resilience factors and have increased the risk of behavioral addictions. Future research on resilience factors and effective prevention and treatment interventions could help AIANs avoid behavioral addictions.
Association of Anthropometrics and Body Composition with Maximal and Relative Force and Power of Kayak Stroke in Competitive Kayak Athletes
Authors: Kukić Filip , Petrović Miloš , Greco Gianpiero , Cataldi Stefania , Fischetti Francesco
Published: March 2022

This study determined to what degree anthropometrics and body composition are associated with force and power outputs of a single-stroke kayak test (SSKT). Body height (BH), sitting height, biacromial distance, arm span, stroke length, body mass index (BMI), percent of skeletal muscle mass (PSMM), skeletal muscle mass index (SMMI), percent body fat (PBF) and maximal and relative force (SSKTFmax and SSKTFrel) and power (SSKTPmax and SSKTPrel) of the SSKT were assessed in 21 male kayak competitors, including sprint specialists and long-distance specialists. Correlation analysis established the association (p < 0.05) between SSKTFmax and BM (r = 0.511), BMI (r = 0.495) and SMMI (r = 0.530). A significant correlation (p < 0.05) also occurred between SSKTPmax and BMI (r = 0.471) and SMMI (r = 0.489). Regression analysis determined a significant association of the anthropometrics–body composition model of kayakers with SSKTFmax (R2 = 0.790), SSKTFRel (R2 = 0.748), SSKTPmax (R2 = 0.676) and SSKTPrel (R2 = 0.625). A longer and wider upper body supported by higher amounts of skeletal muscle mass per square of body size provides higher force outcomes in a complex single-handed SSKT, while the PSMM provides higher outcomes in SSKTPmax.