bluuebunny/crossref_metadata_2025 huggingface dataset:
Intensitas Komunikasi dan Keberhasilan Pemberdayaan Masarakat Berbasis Pendampingan di Desa Wisata Selasari-Kabupaten Pangandaran
Authors: Simanjuntak Diana
Published: March 2022

This study aims to explore the intensity of communication and the success of mentoring-based community empowerment in the Selasari Tourist Village - Pangandaran Regency. This research was a qualitative descriptive with a case study approach. The research data collection techniques was through in-depth interviews, participatory observation, document studies and literature studies. The research informants were 20 people of the community of Selasari Tourism Village, Pangandaran Regency who had participated in a community empowerment program based on assistance. However, there were only 5 key informants selected consisted of tourist village managers, UMKM managers, homestay managers and tour guides as well as the Head of Selasari Village. The results showed that the four months intensity of communication between companions and participants greatly supported the success of community empowerment. It was evident from their ability to take part in the Indonesian Tourism Village Appreciation (ADWI)  and Sustainable Tourism Village Certification competitions in 2021. Informants also explained that compared to community empowerment by various parties prior which did not bring much change for them, the assistance approach was very useful for their ability in developing their village. The intensity of communication carried out by the facilitators in the empowerment process consists of: the frequency of repeated communication, the duration of communication that describes the length of the meeting, the familial attention of the facilitators toward the community, regularity in communication in the form of a clear and definite schedule, the message breadth level and the limited number of participants in communication, and the message depth level. All the mentioned can encourage the community empowerment participants to achieve optimal mentoring results.
Nilai-Nilai Moral dan Spiritual; Pengaruh Wisata Religi Terhadap Peluang Ekonomi Rakyat
Authors: Sartika Iriany Ieke , Pasciana Rostiena , Mulyaningsih Mulyaningsih , Febrina Ismira
Published: March 2022

The tourists invasion to the sanctuary of the Islam spreaders, it is the social dynamics to strenghten faith. West Java is one of the provinces that has the potential for religious tourism which is much loved by the Indonesian people. This attracts researchers to examine more deeply the relationship between religious tourism and people's economic opportunities. This study aims to describe and analyze the influence of religious tourism on the people's economic opportunities. Religious tourism in West Java are mostly visited. In this situation, people’s economic opportunities can be captured by the local people, such as the souvenirs and flowers sellers. This research uses a quantitative approach to find out how significant the influence of Religious Tourism on the people's economic opportunities is and analyze it through the sociology of religion. The population in this study are people who work as traders who sell at the research location; with a sample of 59 people obtained through simple random cluster sampling. Based on the results of the analysis that there is a influence of religious tourism on the community economic opportunities, which is equal to 32.15%. Meanwhile, 67, 85% were influenced by other factors not examined in this study. From the effectiveness of religious sociology, and Parson's theory as a social system, which is explored through analysis of Adaptation, Goal Attainment, integration and Latent Pattern Maintenance is very relevant. seizing people's economic opportunities by adapting tourism elements, as well as trying to carry out cultural and religious attractions, such as solemn and silent prayer rituals, in accordance with tourist expectations. This pilgrimage tourism activity can integrate with tourist destinations and can carry out the maintenance of local cultural patterns. However, to increase the potential for pilgrimage tourism, it is still necessary to maintain a better environment and order.
Dampak Wisata Religi Makam Eyang Kobul Ditinjau dari Aspek Ekonomi dan Sosial
Authors: Kurniawan Kunkun , Suganda Dadang , Lies Siti Khadijah Ute
Published: March 2022

Tourism is a travel activity carried out by a person or group to a place to gain satisfaction, enjoyment, happiness, experience or new knowledge. Tourism activities are not only to fulfill material needs but also spiritually. The spiritual needs of a person or group come from something non-physical. One of these non-physical needs is the desire to always want to be close to people who are part of the family, figures, leaders who are honored for their services and sacrifices even though they have been physically cut off in the real world. Pilgrimage to the grave is a tradition carried out by a person or group to attribute glory to the ancestors, showing respect for a figure or leader who has contributed to visiting the glorified and sacred tomb. This study aims to determine the historical background of the Eyang Kobul gravesite and its impact on economic and social life for the surrounding community. Furthermore, the method used in this research is to use qualitative methods. The research data were collected through observation, interviews, and study of literature. The results of this study indicate that the existence of the Eyang Kobul gravesite has a positive impact on the economy and social life of the community around Jatisari Village, Kutawaringin District, Bandung Regency. The future challenge related to the development of the Pilgrimage tour of the Tomb of Eyang Kobul is how the manager with the principle of independence can realize a program of arrangement and improvement of better facilities for pilgrims.
La mejora continua: reflexiones del seguimiento en programas educativos de nivel superior con el marco de referencia CONAIC
Authors: Ochoa Oliva M.J.A. , Reyes Martínez A. , Burgos Sánchez B.A. , Arán Sánchez L.R.
Published: March 2022

El Consejo Nacional de Acreditación en Informática y Computación A.C. (CONAIC) cuenta con las etapas generales en su proceso de evaluación con fines de la acreditación: solicitud, autoevaluación, evaluación externa, dictamen y seguimiento para la mejora continua. En esta última etapa, se presentan observaciones y/o recomendaciones en base a los resultados obtenidos en diversas evaluaciones de la Comisión Técnica de la visita de evaluación, seguimiento de recomendaciones y observaciones de los programas educativos; se verifica el cumplimiento de las mismas, cuando la institución ejecuta el plan de mejora y notifica al consejo los avances en la atención a las recomendaciones, mediante las evidencias y elaboración de informes periódicos.

The National Council of Accreditation in Computing and Computing A.C. (CONAIC) has the general stages in its evaluation process for accreditation purposes: application, self-evaluation, external evaluation, opinion and monitoring for continuous improvement. In this last stage, observations and / or recommendations are presented based on the results obtained in various evaluations of the Technical Commission of the evaluation visit, follow-up of recommendations and observations of the educational programs; the fulfillment of the same is verified, when the institution executes the plan of improvement and notifies to the advice the advances in the attention to the recommendations, by means of the evidences and preparation of periodic reports.
Treating COVID with Bike Lanes: Design, Spatial, and Network Analysis of ‘Pop-Up’ Bike Lanes in Paris
Authors: Moran Marcel E.
Published: March 2022

Paris bypassed standard-construction processes to rapidly expand its bike-lane network amidst COVID-19. This study analyses these new lanes in terms of their design, spatial footprint, and relationship to the pre-COVID network. Municipal data, street imagery, and in-person observations demonstrate that Paris’s new ‘pop-up’ lanes are a higher share bi-directional than the pre-COVID network (49% vs. 39%), a higher share more protected (77% vs. 73%), and average a higher number of interconnections. These 47 kilometers connect the city’s peripheral ring to its inner core, primarily represent new lanes as opposed to upgrades of existing lanes (66% vs. 33%), and replace both traffic lanes and on-street parking.
Perceptions of the Yangtze River cruise ship brand based on text semantic analysis: A web big data perspective
Authors: Tong Helong , Yu Xian , Xu Wenjing , Li Hua
Published: March 2022

Relying on the rich natural and human resources of the Yangtze River, the Yangtze River cruise has been a popular and famous tourism route. This study adopts a big data approach to study the perception of and satisfaction with the Yangtze River cruise brand, so as to come up with some practical suggestions for the enhancement of its value. Based on the analysis of the current development of the Yangtze River cruise tour, this paper conducted a textual analysis on 2,260 user reviews of two important Chinese travel OTA companies (Ctrip.com and Tuniu.com) through the theory and method of web text mining. A word frequency analysis, the construction of semantic networks, and a sentiment analysis were carried out. The results of the study show that the market perception of each cruise company’s brand is not very different, and the relevant companies have not yet developed their own special products and services. “Service” is the most frequently mentioned high-frequency word in tourists’ reviews. The sentiment analysis shows that the frequency of positive emotions in tourists’ reviews is high (exceeding 80 %). Through the satisfaction analysis of six dimensions of Yangtze River cruises, it is found that “cruise dining” and “cruise entertainment” are weak points.
Numerical Stress Analysis of Creep-Feed Grinding Through Finite Element Method in Inconel Alloy X-750
Authors: Jafarpour Vahid , Moharrami Rasoul
Published: March 2022

The present study developed a 2D finite element model for simulation of creep-feed grinding process. Fully-coupled mechanical-thermal analysis was used to predict the residual stresses distribution. As in the creep-feed grinding the thermal damages are considerable, so the best and worst cooling condition i.e. flood and dry grindings were studied. Convection heat transfer coefficient was utilized to shows the effect of coolant. The results show the maximum temperature of specimen has reduced by about 52% compared to non-use of coolant. The dominate residual stresses are tensile near the surface that a steep decline in stresses was observed in flood grinding. Also, by using the electro polishing layer removal technique the non-uniform residual stresses were measured to validate the model. The results demonstrated the presented model provides good congruency with the experiments
PIP5K1α is Required for Promoting Tumor Progression in Castration-Resistant Prostate Cancer
Authors: Wang Tianyan , Sarwar Martuza , Whitchurch Jonathan B , Collins Hilary M , Green Tami , Semenas Julius , Ali Amjad , Roberts Christopher J , Morris Ryan D , Hubert Madlen , Chen Sa , El-Schich Zahra , Wingren Anette G , Grundström Thomas , Lundmark Richard , Mongan Nigel P , Gunhaga Lena , Heery David M , Persson Jenny L
Published: March 2022

PIP5K1α has emerged as a promising drug target for the treatment of castration-resistant prostate cancer (CRPC), as it acts upstream of the PI3K/AKT signaling pathway to promote prostate cancer (PCa) growth, survival and invasion. However, little is known of the molecular actions of PIP5K1α in this process. Here, we show that siRNA-mediated knockdown of PIP5K1α and blockade of PIP5K1α action using its small molecule inhibitor ISA-2011B suppress growth and invasion of CRPC cells. We demonstrate that targeted deletion of the N-terminal domain of PIP5K1α in CRPC cells results in reduced growth and migratory ability of cancer cells. Further, the xenograft tumors lacking the N-terminal domain of PIP5K1α exhibited reduced tumor growth and aggressiveness in xenograft mice as compared to that of controls. The N-terminal domain of PIP5K1α is required for regulation of mRNA expression and protein stability of PIP5K1α. This suggests that the expression and oncogenic activity of PIP5K1α are in part dependent on its N-terminal domain. We further show that PIP5K1α acts as an upstream regulator of the androgen receptor (AR) and AR target genes including CDK1 and MMP9 that are key factors promoting growth, survival and invasion of PCa cells. ISA-2011B exhibited a significant inhibitory effect on AR target genes including CDK1 and MMP9 in CRPC cells with wild-type PIP5K1α and in CRPC cells lacking the N-terminal domain of PIP5K1α. These results indicate that the growth of PIP5K1α-dependent tumors is in part dependent on the integrity of the N-terminal sequence of this kinase. Our study identifies a novel functional mechanism involving PIP5K1α, confirming that PIP5K1α is an intriguing target for cancer treatment, especially for treatment of CRPC.
Dynamic Changes in the Proteome of Early Bovine Embryos Developed In Vivo
Authors: Banliat Charles , Mahé Coline , Lavigne Régis , Com Emmanuelle , Pineau Charles , Labas Valérie , Guyonnet Benoit , Mermillod Pascal , Saint-Dizier Marie
Published: March 2022

Early embryo development is a dynamic process involving important molecular and structural changes leading to the embryonic genome activation (EGA) and early cell lineage differentiation. Our aim was to elucidate proteomic changes in bovine embryos developed in vivo. Eleven females were used as embryo donors and pools of embryos at the 4–6 cell, 8–12 cell, morula, compact morula and blastocyst stages were analyzed by nanoliquid chromatography coupled with label free quantitative mass spectrometry. A total of 2,757 proteins were identified, of which 1,950 were quantitatively analyzed. Principal component analysis of data showed a clear separation of embryo pools according to their developmental stage. The hierarchical clustering of differentially abundant proteins evidenced a first cluster of 626 proteins that increased in abundance during development and a second cluster of 400 proteins that decreased in abundance during development, with most significant changes at the time of EGA and blastocyst formation. The main pathways and processes overrepresented among upregulated proteins were RNA metabolism, protein translation and ribosome biogenesis, whereas Golgi vesicle transport and protein processing in endoplasmic reticulum were overrepresented among downregulated proteins. The pairwise comparison between stages allowed us to identify specific protein interaction networks and metabolic pathways at the time of EGA, morula compaction and blastocyst formation. This is the first comprehensive study of proteome dynamics in non-rodent mammalian embryos developed in vivo. These data provide a number of protein candidates that will be useful for further mechanistic and functional studies.
Early Change in Area Strain Detected by 3D Speckle Tracking Is Associated With Subsequent Cardiotoxicity in Patients Treated With Low Doses of Anthracyclines
Authors: Piveta Rafael B. , Rodrigues Ana Clara T. , Vieira Marcelo L. C. , Fischer Cláudio H. , Afonso Tania R. , Daminello Edgar , Cruz Felipe M. , Galvão Tatiana F. G. , Filho Edgar B. L. , Katz Marcelo , Morhy Samira S.
Published: March 2022

Objective

To evaluate the prognostic impact of the parameters of myocardial deformation using three-dimensional speckle tracking echocardiography (3DSTE) in patients with breast cancer who underwent chemotherapy with low doses of anthracyclines.

Background

Chemotherapy-related cardiotoxicity has an important prognostic impact on cancer survivors. Three-dimensional STE has revealed more consistent data than two-dimensional techniques and may represent a more accurate tool in the evaluation of myocardial function in patients who underwent chemotherapy.

Methods

We evaluated patients with breast cancer who were treated with anthracyclines (associated or not with trastuzumab) in five stages: baseline, after cumulative doses of 120 and 240 mg/m2 of doxorubicin, and then, after 6 months and at least 1 year after anthracyclines. Ultrasensitive troponin I (US-TnI) and a standard echocardiography study were performed at each stage. We analyzed left ventricular ejection fraction (LVEF) by Simpson's method, two-dimensional speckle tracking (2DSTE) with longitudinal and radial strain values, and 3DSTE with longitudinal, radial, and circumferential strain as well as twist, torsion, rotation, and three-dimensional global area strain (3DGAS). Cardiotoxicity was defined as a decrease in LVEF by more than 10 percentage points to a value lower than 53%.

Results

We evaluated 51 female patients who were aged 50.6 ± 11 years. After the cumulative dose of 240 mg/m2 of doxorubicin, US-TnI was increased (>34 pg/ml) in 21 patients (45%, p > 0.001), LVEF remained unchanged (p = 0.178), while 2DSTE longitudinal strain was decreased (from −17.8% to −17.1%, p < 0.001) and 3DSTE detected changes in longitudinal, radial, circumferential, and area strain. After a lower cumulative dose of doxorubicin (120 mg/m2), 3DGAS (p < 0.001) was the only parameter that was changed. In the follow-up, 7 (13%) patients presented a decrease in LVEF. Three-dimensional GAS early changed to abnormal values was the only variable associated with a subsequent decrease in LVEF (definitive cardiotoxicity).

Conclusion

In patients with breast cancer, 3DSTE detected early changes in area strain after very low doses of doxorubicin. The 3DGAS early changed to abnormal values was associated with a subsequent decrease in LVEF, representing a promising technique to predict chemotherapy-induced cardiomyopathy.
Energy Drinks: Effects on Blood Pressure and Heart Rate in Children and Teenagers. A Randomized Trial
Authors: Oberhoffer Felix Sebastian , Li Pengzhu , Jakob André , Dalla-Pozza Robert , Haas Nikolaus Alexander , Mandilaras Guido
Published: March 2022

Background

The consumption of Energy Drinks (ED) is very popular among children and teenagers. While potential cardiovascular side effects of these beverages are suggested, the acute impact of ED consumption on the pediatric cardiovascular system has not been systematically examined yet. The aim of this study was to investigate the acute effects of ED consumption on blood pressure and heart rate in healthy children and teenagers.

Methods

This study was a randomized, single-blind, placebo-controlled, crossover clinical trial. On two consecutive days, the study participants were asked to consume a weight-adjusted amount of an ED (3 mg caffeine per kg of body weight) or a placebo containing a similar amount of sugar but without conventional ED ingredients. Systolic blood pressure (SBP), diastolic blood pressure (DBP) and heart rate were measured at the following time points: baseline as well as 30, 60, 120 and 240 min after beverage consumption.

Results

In total, 27 healthy children and adolescents (mean age 14.53 ± 2.40 years, 14 male) were included in the present study. Compared to placebo intake, mean SBP was demonstrated to be up to 5.23 mmHg (p < 0.0001) and mean DBP up to 3.29 mmHg (p < 0.001) increased after ED consumption. Prevalence of elevated blood pressure, stage 1 and stage 2 hypertension was higher after ED consumption. Heart rate tended to be lower after ED consumption.

Conclusions

The acute ED consumption is associated with a significantly increased SBP and DBP in healthy children and teenagers. Minors, particularly those with pre-existing health conditions, should be discouraged from drinking EDs.

Clinical Trial Registration

https://www.drks.de/drks_web/, identifier: DRKS00027580.
The Dynamic of PRAMEY Isoforms in Testis and Epididymis Suggests Their Involvement in Spermatozoa Maturation
Authors: Kern Chandlar H. , Feitosa Weber B. , Liu Wan-Sheng
Published: March 2022

The preferentially expressed antigen in melanoma, Y-linked (PRAMEY) is a cancer/testis antigen expressed predominantly in bovine spermatogenic cells, playing an important role in germ cell formation. To better understand PRAMEY’s function during spermatogenesis, we studied the dynamics of PRAMEY isoforms by Western blotting (WB) with PRAMEY-specific antibodies. The PRAMEY protein was assessed in the bovine testicular and epididymal spermatozoa, fluid and tissues, and as well as in ejaculated semen. The protein was further examined, at a subcellular level in sperm head and tail, as well as in the subcellular components, including the cytosol, nucleus, membrane, and mitochondria. RNA expression of PRAMEY was also evaluated in testis and epididymal tissues. Our WB results confirmed the previously reported four isoforms of PRAMEY (58, 30, 26, and 13 kDa) in the bovine testis and spermatozoa. We found that testicular spermatozoa expressed the 58 and 30 kDa isoforms. As spermatozoa migrated to the epididymis, they expressed two additional isoforms, 26 and 13 kDa. Similarly, the 58 and 30 kDa isoforms were detected only in the testis fluid, while all four isoforms were detected in fluid from the cauda epididymis. Tissue evaluation indicated a significantly higher expression of the 58 and 13 kDa isoforms in the cauda tissue when compared to both the testis and caput tissue (p < 0.05). These results indicated that testis samples (spermatozoa, fluid, and tissue) expressed predominantly the 58 and 30 kDa PRAMEY isoforms, suggesting their involvement in spermatogenesis. In contrast, the 26 kDa isoform was specific to epididymal sperm and the 13 kDa isoform was marked in samples derived from the cauda epididymis, suggesting their involvement in sperm maturation. Results from the sperm head and tail experiments indicated that the 13 kDa isoform increased 4-fold in sperm tails from caput to cauda, suggesting this isoform may have a significant role in tail function. Additionally, the 13 kDa isoform increased significantly (p < 0.05) in the cytosol during epididymal passage and tended to increase in other subcellular components. The expression of PRAMEY in the sperm subcellular components during epididymal maturation suggests the involvement of PRAMEY, especially the 13 kDa isoform, in sperm motility.
Activation of Canine, Mouse and Human TLR2 and TLR4 by Inactivated Leptospira Vaccine Strains
Authors: Novak Andreja , Pupo Elder , van’t Veld Esther , Rutten Victor P. M. G. , Broere Femke , Sloots Arjen
Published: March 2022

CanineLeptospiravaccines contain inactivated strains of pathogenicLeptospira, the causative agents of leptospirosis. For an effective response to vaccination, activation of the innate immune systemviapattern recognition receptors such as TLRs is crucial. However, it is not known which TLRs are activated byLeptospirain dogs. To investigate the involvement of canine TLR2, TLR4, and TLR5 in the recognition ofLeptospira, we stimulated canine moDC and reporter cells expressing canine TLR2 with either whole-inactivated bacteria or purified LPS ofLeptospirastrains, representing the serogroups generally used in canine leptospirosis vaccines. Using the endotoxin neutralizing reagent polymyxin B and TLR4 antagonist RS-LPS, we demonstrate thatLeptospiraLPS and canine TLR4 are involved in IL-1β production as well as in the uptake of inactivatedLeptospirain canine moDC. Furthermore, polymyxin B only partially inhibited IL-1β production induced by inactivatedLeptospira, suggesting that next to TLR4, also other TLRs may be involved. The observed activation of canine TLR2-expressing reporter cells by inactivatedLeptospirastrains indicates that TLR2 could be one of these TLRs. Next, we analyzed TLR2 and TLR4 activating capabilities by the sameLeptospirastrains using human and mouse TLR-expressing reporter cells. InactivatedLeptospiraand leptospiral LPS activated not only mouse, but also human TLR4 and this activation was shown to be LPS dependent in both cases. Additionally, inactivatedLeptospiraactivated mouse and human TLR2-expressing reporter cell lines. In our study, we could not identify significant species differences in the recognition ofLeptospiraby TLR2 and TLR4 between dog, human and mouse. Lastly, we show that these inactivatedLeptospirastrains are recognized by both mouse and human TLR5 reporter cells only after exposure to additional heat-treatment. Unfortunately, we were not able to confirm this in the canine system. Our data show that TLR2 and TLR4 are involved in the recognition ofLeptospirastrains used in the production of canineLeptospiravaccines. This study contributes to the understanding ofLeptospira-induced innate immune responses in dogs, humans, and mice. Future studies are needed to further explore the role of canine TLR2, TLR4 and TLR5 in the induction of vaccine-mediated immunity againstLeptospira.
Trajectory of IgG to SARS-CoV-2 After Vaccination With BNT162b2 or mRNA-1273 in an Employee Cohort and Comparison With Natural Infection
Authors: Keshavarz Behnam , Richards Nathan E. , Workman Lisa J. , Patel Jaimin , Muehling Lyndsey M. , Canderan Glenda , Murphy Deborah D. , Brovero Savannah G. , Ailsworth Samuel M. , Eschenbacher Will H. , McGowan Emily C. , Mann Barbara J. , Nelson Michael R. , Kadl Alexandra , Woodfolk Judith A. , Platts-Mills Thomas A.E. , Wilson Jeffrey M.
Published: March 2022

Three COVID-19 vaccines have received FDA-authorization and are in use in the United States, but there is limited head-to-head data on the durability of the immune response elicited by these vaccines. Using a quantitative assay we studied binding IgG antibodies elicited by BNT162b2, mRNA-1273 or Ad26.COV2.S in an employee cohort over a span out to 10 months. Age and sex were explored as response modifiers. Of 234 subjects in the vaccine cohort, 114 received BNT162b2, 114 received mRNA-1273 and six received Ad26.COV2.S. IgG levels measured between seven to 20 days after the second vaccination were similar in recipients of BNT162b2 and mRNA-127 and were ~50-fold higher than in recipients of Ad26.COV2.S. However, by day 21 and at later time points IgG levels elicited by BNT162b2 were lower than mRNA-1273. Accordingly, the IgG decay curve was steeper for BNT162b2 than mRNA-1273. Age was a significant modifier of IgG levels in recipients of BNT162b2, but not mRNA-1273. After six months, IgG levels elicited by BNT162b2, but not mRNA-1273, were lower than IgG levels in patients who had been hospitalized with COVID-19 six months earlier. Similar findings were observed when comparing vaccine-elicited antibodies with steady-state IgG targeting seasonal human coronaviruses. Differential IgG decay could contribute to differences observed in clinical protection over time between BNT162b2 and mRNA-1273.
PSRR: A Web Server for Predicting the Regulation of miRNAs Expression by Small Molecules
Authors: Yu Fanrong , Li Bihui , Sun Jianfeng , Qi Jing , De Wilde Rudy Leon , Torres-de la Roche Luz Angela , Li Cheng , Ahmad Sajjad , Shi Wenjie , Li Xiqing , Chen Zihao
Published: March 2022

Background: MicroRNAs (miRNAs) play key roles in a variety of pathological processes by interacting with their specific target mRNAs for translation repression and may function as oncogenes (oncomiRs) or tumor suppressors (TSmiRs). Therefore, a web server that could predict the regulation relations between miRNAs and small molecules is expected to achieve implications for identifying potential therapeutic targets for anti-tumor drug development.

Methods: Upon obtaining positive/known small molecule-miRNA regulation pairs from SM2miR, we generated a multitude of high-quality negative/unknown pairs by leveraging similarities between the small molecule structures. Using the pool of the positive and negative pairs, we created the Dataset1 and Dataset2 datasets specific to up-regulation and down-regulation pairs, respectively. Manifold machine learning algorithms were then employed to construct models of predicting up-regulation and down-regulation pairs on the training portion of pairs in Dataset1 and Dataset2, respectively. Prediction abilities of the resulting models were further examined by discovering potential small molecules to regulate oncogenic miRNAs identified from miRNA sequencing data of endometrial carcinoma samples.

Results: The random forest algorithm outperformed four machine-learning algorithms by achieving the highest AUC values of 0.911 for the up-regulation model and 0.896 for the down-regulation model on the testing datasets. Moreover, the down-regulation and up-regulation models yielded the accuracy values of 0.91 and 0.90 on independent validation pairs, respectively. In a case study, our model showed highly-reliable results by confirming all top 10 predicted regulation pairs as experimentally validated pairs. Finally, our predicted binding affinities of oncogenic miRNAs and small molecules bore a close resemblance to the lowest binding energy profiles using molecular docking. Predictions of the final model are freely accessible through the PSRR web server at https://rnadrug.shinyapps.io/PSRR/.

Conclusion: Our study provides a novel web server that could effectively predict the regulation of miRNAs expression by small molecules.
Guhong Injection Prevents Ischemic Stroke-Induced Neuro-Inflammation and Neuron Loss Through Regulation of C5ar1
Authors: Zhang Jingjing , Zhou Rui , Cao Guangzhao , Zhang Yi , Xu He , Yang Hongjun
Published: March 2022

C5ar1 (CD88) has been identified as an important potential therapeutic target for regulating inflammation in ischemic stroke. In this study, the neuroprotective effect of Guhong injection (GHI) on middle cerebral artery occlusion (MCAO)-induced reperfusion injury was assessed and the mechanism was explored by RNA-seq technology. GHI administered for 6 consecutive days significantly decreased body weight loss, infarction rate, neurological deficient scores, and neuron loss but improved rat survival percentage and regional cerebral blood flow after MCAO surgery. Furthermore, we identified inflammation as a vital process and C5AR1 as a vital target in GHI-mediated protection by using RNA-seq analysis. Further experiments confirmed that GHI decreased C5AR1, C5A, CASP3, 8-OHdG, and inflammatory factors such as IL-1β, TNF, IL6, ICAM-1, MMP9, and MCP-1, and enhanced the expression of TIMP1, JAM-A, and laminin. Furthermore, GHI and its major components hydroxysafflower yellow A (HSYA) and aceglutamide (AG) enhanced cell viability and reduced LDH level and C5AR1 expression in a C5A-induced Neuro-2a cell damage model. In general, this study elucidated the mechanism of GHI against ischemic stroke by inhibiting inflammation and highlighted the potential important role of C5AR1 in ischemic stroke. This research provided new insights into the mechanism of GHI in resisting ischemic stroke and benefits of its clinical application.
HSP90 Inhibitors Modulate SARS-CoV-2 Spike Protein Subunit 1-Induced Human Pulmonary Microvascular Endothelial Activation and Barrier Dysfunction
Authors: Colunga Biancatelli Ruben Manuel Luciano , Solopov Pavel A. , Gregory Betsy , Khodour Yara , Catravas John D.
Published: March 2022

Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has caused more than 5 million deaths worldwide. Multiple reports indicate that the endothelium is involved during SARS-Cov-2-related disease (COVID-19). Indeed, COVID-19 patients display increased thrombophilia with arterial and venous embolism and lung microcapillary thrombotic disease as major determinants of deaths. The pathophysiology of endothelial dysfunction in COVID-19 is not completely understood. We have investigated the role of subunit 1 of the SARS-CoV-2 spike protein (S1SP) in eliciting endothelial barrier dysfunction, characterized dose and time relationships, and tested the hypothesis that heat shock protein 90 (HSP90) inhibitors would prevent and repair such injury. S1SP activated (phosphorylated) IKBα, STAT3, and AKT and reduced the expression of intercellular junctional proteins, occludin, and VE-cadherin. HSP90 inhibitors (AT13387 and AUY-922) prevented endothelial barrier dysfunction and hyperpermeability and reduced IKBα and AKT activation. These two inhibitors also blocked S1SP-mediated barrier dysfunction and loss of VE-cadherin. These data suggest that spike protein subunit 1 can elicit, by itself, direct injury to the endothelium and suggest a role of HSP90 inhibitors in preserving endothelial functionality.
OsAPX1 Positively Contributes to Rice Blast Resistance
Authors: Sheng Cong , Yu Dongli , Li Xuan , Yu Hanxi , Zhang Yimai , Saqib Bilal Muhammad , Ma Hongyu , Zhang Xin , Baig Ayesha , Nie Pingping , Zhao Hongwei
Published: March 2022

Ascorbate peroxidases (APXs) maintain cellular reactive oxygen species (ROS) homeostasis through their peroxidase activity. Here, we report that OsAPX1 also promotes ROS production such that a delicate cellular ROS homeostasis is achieved temporally after Magnaporthe oryzae infection. OsAPX1 specifically induces ROS production through increasing respiratory burst oxidase homologs (OsRBOHs) expression and can be inhibited by DPI, a ROS inhibitor. The time-course experiment data show that the simultaneous induction of OsAPX1 and OsRBOHs leads to ROS accumulation at an early stage; whereas a more durable expression of OsAPX1 leads to ROS scavenging at a later stage. By the temporal switching between ROS inducer and eliminator, OsAPX1 triggers an instant ROS burst upon M. oryzae infection and then a timely elimination of ROS toxicity. We find that OsAPX1 is under the control of the miR172a-OsIDS1 regulatory module. OsAPX1 also affects salicylic acid (SA) synthesis and signaling, which contribute to blast resistance. In conclusion, we show that OsAPX1 is a key factor that connects the upstream gene silencing and transcription regulatory routes with the downstream phytohormone and redox pathway, which provides an insight into the sophisticated regulatory network of rice innate immunity.
Herd Behaviour, Fundamental, and Macroeconomic Variables – The Driving Forces of Stock Returns: A Panel-Based Pooled Mean Group Approach
Authors: Jabeen Shaista , Rizavi Sayyid Salman , Farhan Muhammad
Published: March 2022

The existing research aims to seek the herding effects on stock returns at the industry level in Pakistan Stock Exchange (PSX). Moreover, the relationship between stock returns and herding has been studied by taking some macroeconomic (exchange rate, interest rate, and inflation rate) and fundamental (return on equity and earnings per share) control variables. Herding is actually imitating other’s behaviour. This phenomenon indicates a situation where the investors follow the crowed and ignores their personal information, despite knowing the correctness of their information. Herd behaviour may drive from fundamental factors leading to efficient markets. However, it may not be associated with fundamental information causing unstable prices. The stock price data of PSX listed companies from 1999 to 2017 have been the point of focus. The underlying herding measure was the cross-sectional absolute deviation (CSAD), proposed by Chang et al. (2000). The significant analysis technique facilitating the current research is pooled mean group (PMG)/panel autoregressive distributed lag (ARDL) approach. Findings revealed that some sectors are evident for positive effect of herding on stock returns, whereas some others are witnessed for its negative effects on stock returns, in both long run and short run. As far as the control variables are concerned, they demonstrated both significant and insignificant effects on stock returns in different sectors of PSX. The study has important implications for policymakers.
Occupational Stress and the Quality of Life of Nurses in Infectious Disease Departments in China: The Mediating Role of Psychological Resilience
Authors: Yan Jiaran , Wu Chao , Du Yanling , He Shizhe , Shang Lei , Lang Hongjuan
Published: March 2022

Aim

We aim to explore the impact of occupational stress on the quality of life of nurses in infectious disease departments and to explore the mediating role of psychological resilience on this impact.

Background

Sudden public health events and the prevalence of infectious diseases give nurses in infectious disease departments a heavy task load and high occupational stress, which can affect their quality of life, and which is closely related to the quality of clinical care they provide. There are few existing studies on occupational stress, psychological resilience, and the quality of life of nurses in infectious disease departments.

Methods

We collected data from infectious-disease-specialized hospitals or infectious disease departments of general hospitals in China. In total 1,536 nurses completed questionnaires: the Effort-Reward Imbalance Questionnaire, the Connor-Davidson Resilience Scale, and the World Health Organization Quality of Life Brief Scale. We use a structural equation model to test the mediating role of the psychological resilience in the relationship between occupational stress and quality of life.

Results

Among 1,536 participants, 88.2% experienced an effort-reward imbalance. The average scores for psychological resilience and quality of life were 56.06 (SD = 14.19) and 51.80 (SD = 8.23), respectively. Our results show that occupational stress is negatively correlated with psychological resilience (r = −0.28, p < 0.01) and quality of life (r = −0.44, p < 0.01). In addition, we find that psychological resilience is positively correlated with quality of life (r = 0.55, p < 0.01) and that the indirect effect of occupational stress on quality of life through psychological resilience is significant (β = −0.036, 95% CI: 0.027 to 0.426), indicating at least a partial mediating role of psychological resilience.

Conclusion

A high proportion of nurses in infectious disease departments felt that their jobs’ effort-reward imbalance was high. These nurses’ scores for psychological resilience were in the middle level among Chinese people generally, but their quality of life was lower than the Chinese norm. We conclude that occupational stress has an important impact on their quality of life, and psychological resilience plays a partial mediating role on this impact.

Implications for Nursing Management

Hospital managers can benefit from paying attention to the occupational stress of nurses and helping to improve the quality of life of nurses by alleviating this occupational stress and improving psychological resilience.
Individual Preventive Behaviors of COVID-19 and Associated Psychological Factors Among Chinese Older Adults: A Cross-Sectional Online Survey
Authors: Duan Yanping , Hu Chun , Lin Zhihua , Liang Wei , Shang Borui , Baker Julien Steven , He Jiali , Wang Yanping
Published: March 2022

Purpose

Older adults aged 60 years and above are classified as being of high-risk for infection during the COVID-19 pandemic. This study aimed to investigate the associations of psychological factors (motivational factors: risk perception, health knowledge, attitude, subjective norm, motivational self-efficacy, and intention; volitional factors: volitional self-efficacy, planning, and action control) of preventive behaviors with three preventive behaviors (hand washing, facemask wearing, and social distancing) among Chinese older adults during the COVID-19 pandemic.

Methods

A cross-sectional questionnaire survey was administered via SOJUMP, a widely used online survey platform in China. A total of 928 older adults (mean = 67.24 years, age range: 60–90 years, SD = 6.43, 55.9% females) were recruited using a snowball sampling approach from Hubei Province (n = 667) and outside Hubei Province (n = 261) in China during May 18, 2020 to June 7, 2020. Multiple hierarchical regressions were conducted with four models to examine the association between demographic, past behavior, psychological factors and each preventive behavior.

Principal Findings

All three preventive behaviors in older adults increased dramatically during the pandemic of COVID-19. Gender, living status, educational level, past behavior, health knowledge, intention and planning significantly predicted hand washing behavior, R2 = 0.395, F(10, 927) = 54.372, p < 0.001. Gender, education level, important others (e.g., family members or friends) infection, past behavior, health knowledge, planning and action control significantly predicted mask wearing behavior, R2 = 0.202, F(10, 927) = 23.197, p < 0.001. Living place, past behavior and health knowledge significantly predicted social distancing behavior, R2 = 0.204, F(9, 927) = 26.201, p < 0.001.

Major Conclusions

Past behavior and health knowledge predicted all three preventive behaviors. Planning was an important psychological factor for both hand washing and mask wearing behaviors. All those critical demographic and psychological factors are critical for future interventions to facilitate older adults to comply with three preventive behaviors in daily life and to stay healthy during the COVID-19 pandemic.
Exploring the Relationships Among Experiences in Nature, Wellbeing, and Stewardship During the COVID-19 Pandemic
Authors: Heilmayr Dietlinde , Baranski Erica N. , Miller Travis J.
Published: March 2022

The COVID-19 pandemic brought sudden and dramatic changes to our daily lives. From shifting to remote work, to following shelter-in-place orders, to increased concerns about the health and wellbeing of one's self and family, individuals were required to make changes to their daily habits and to find new methods of coping with stress and maintaining wellbeing. In the present study, we surveyed participants in the United States (N= 192) with open-ended questions and individual difference measures to capture how changes to daily life due to the COVID-19 pandemic affected individuals' engagement with the outdoors. Specifically, using descriptive and inferential statistics, we (1) describe how people experienced the outdoors during the beginning stages of the COVID-19 pandemic; (2) evaluate how individuals' experiences outdoors relate to individual differences; and (3) report whether environmental experiences and COVID-19 concern relate to whether individuals chose to donate their participation payment to The Trust for Public Land, to the Center for Disease Control's COVID-19 fund, or to keep the payment for themselves in the form of a gift card. This work enhances our understanding of how the pandemic affected the relationship between people and the outdoors and contributes to knowledge about how nature can be used to help individuals and communities during times of crisis.
Predicting the Rate Coefficients of Attachment and Detachment of Colloids in Saturated Porous Media
Authors: Krishna Yerramilli Sai Rama , Seetha N.
Published: March 2022

The transport behavior of pathogenic microorganisms and nanoparticles (NPs) in the subsurface is usually studied by performing laboratory soil column experiments. Parameters describing colloid deposition on grain surfaces are estimated by fitting observed breakthrough curves with an appropriate one-dimensional model. However, predictive tools to estimate colloid deposition parameters, knowing the system properties such as soil type, colloid type, solution chemistry, and flow velocity, are useful in estimating the travel distances of pathogenic microorganisms in the subsurface. Such predictive models are rare, except the colloid attachment rate coefficient predicted by colloid filtration theory (CFT) under favorable conditions. Although a couple of theoretical and empirical predictive models are available for estimating the deposition parameters under unfavorable conditions, they were developed for a small set of data, and their applicability to a wide range of conditions is unexplored. In this study, several sets of column-experimental data from literature, covering a wide range of experimental conditions, were analyzed to understand key factors that control colloid deposition. Empirical relationships were developed for deposition rate coefficients and sticking efficiency of various colloidal types [viruses, bacteria, graphene oxide (GO) NPs, silver (Ag) NPs, titanium dioxide (TiO2) NPs, and carboxylate-modified latex (CML) colloids] vis-à-vis 11 physicochemical parameters such as porosity, mean pore-water velocity, median grain size, colloid radius, solution ionic strength, surface potentials of colloids and grains, Hamaker constant, temperature, viscosity of water, and dielectric constant. While deposition of viruses and CML colloids on grain surfaces was found to be reversible, deposition of bacteria, GO NPs, Ag NPs, and TiO2 NPs was found to be irreversible. The empirical equations proposed in this study can predict deposition rate coefficients more closely (p < .001, R2 = 0.69−0.85) than CFT (p < .7, R2 ≤ 0.41). The performance of CFT in predicting the attachment rate coefficients of viruses, bacteria, GO NPs, TiO2 NPs, and CML colloids was found to improve significantly when estimated rate coefficients were multiplied by the sticking efficiency calculated using the empirical expression proposed in this study (p < .001, R2 = 0.65−0.95).
Atomic Oxygen SAO, AO and QBO in the Mesosphere and Lower Thermosphere Based on Measurements from SABER on TIMED during 2002–2019
Authors: Gu Shengyang , Zhao Han , Wei Yafei , Wang Dong , Dou Xiankang
Published: March 2022

Using version 1.0 of TIMED/SABER nighttime O(3P) density data in the mesosphere and lower thermosphere (MLT) retrieved from 2.0 and 1.6 μm radiances, we conducted a study on the semiannual oscillation (SAO), annual oscillation (AO) and quasi-biennial oscillation (QBO) of the atomic oxygen volume mixing ratio at 96 km, from 40° S to 40° N, for 2002–2019. We first analyzed the altitude profiles of the atomic oxygen volume mixing ratio and kinetic temperature, and chose to study the daily average of the atomic oxygen volume mixing ratio at 96 km. For the analysis of SAO and AO, we fitted two sinusoidal functions with periods of 6 and 12 months to the daily mean atomic oxygen volume mixing ratio to obtain the annual and semiannual amplitude. The SAO amplitudes had two peaks of 1.68 × 10−3 and 1.63 × 10-3 at about 25° S and 25° N, and displayed a clear hemispheric symmetry. The AO amplitude increased with the latitude and showed distinct minima (valleys) of 3.36 × 10−4 around the equator, as well as a clear hemispheric asymmetry. The correlation coefficient between the atomic oxygen volume mixing ratio QBO with equatorial stratospheric QBO was higher in the tropics than the mid latitudes.
A Novel Un-Supervised GAN for Fundus Image Enhancement with Classification Prior Loss
Authors: Chen Shizhao , Zhou Qian , Zou Hua
Published: March 2022

Fundus images captured for clinical diagnosis usually suffer from degradation factors due to variation in equipment, operators, or environment. These degraded fundus images need to be enhanced to achieve better diagnosis and improve the results of downstream tasks. As there is no paired low- and high-quality fundus image, existing methods mainly focus on supervised or semi-supervised learning methods for color fundus image enhancement (CFIE) tasks by utilizing synthetic image pairs. Consequently, domain gaps between real images and synthetic images arise. With respect to existing unsupervised methods, the most important low scale pathological features and structural information in degraded fundus images are prone to be erased after enhancement. To solve these problems, an unsupervised GAN is proposed for CFIE tasks utilizing adversarial training to enhance low quality fundus images. Synthetic image pairs are no longer required during the training. A specially designed U-Net with skip connection in our enhancement network can effectively remove degradation factors while preserving pathological features and structural information. Global and local discriminators adopted in the GAN lead to better illumination uniformity in the enhanced fundus image. To better improve the visual quality of enhanced fundus images, a novel non-reference loss function based on a pretrained fundus image quality classification network was designed to guide the enhancement network to produce high quality images. Experiments demonstrated that our method could effectively remove degradation factors in low-quality fundus images and produce a competitive result compared with previous methods in both quantitative and qualitative metrics.