bluuebunny/crossref_metadata_2025 huggingface dataset:
The Beneficial Effects of Alpha-Blockers, Antimuscarinics, Beta 3-Agonist, and PDE5-Inhibitors for Ureteral Stent-Related Discomfort: A Systematic Review and Meta-Analysis from KSER Update Series
Authors: Moon Young Joon , Chung Doo Yong , Kim Do Kyung , Jung Hae Do , Jeon Seung Hyun , Kang Seok Ho , Paick Sunghyun , Lee Joo Yong
Published: January 2025

Background and Objectives: Ureteral stents are widely used in the field of urology but can cause varying degrees of side effects. This study utilized a network meta-analysis to evaluate stent-related discomfort (SRD) in patients with alpha-blockers (alfuzosin, tamsulosin, and silodosin), antimuscarinics (solifenacin), beta 3-agonists (mirabegron), and phosphodiesterase 5-inhibitors (tadalafil) versus a placebo. Materials and Methods: Relevant randomized controlled trials (RCTs) from 2006 to 2021 were identified from electronic databases, including PubMed, EMBASE, and the Cochrane Library. The following identifiers were included to assess the urinary symptom score (USS): participants (patients with ureteral stents), interventions (patients who took medication for stent discomfort), and outcomes (comparisons of the Ureteric Stent Symptoms Questionnaire (USSQ)). We also executed an independent quality assessment using the Scottish Intercollegiate Guidelines Network (SIGN). Results: A total of 16 RCTs were identified, and they included 1865 patients. Compared with the placebo, mirabegron (mean difference (MD): −3.87; 95% confidence interval (CI): −10.6–2.35), tadalafil (MD: −4.47; 95% CI: −10.8–1.63), and silodosin (MD: −4.02; 95% CI: −12–4.01) did not show significant differences to the placebo, whereas others did. Alfuzosin, mirabegron, silodosin, solifenacin, and tadalafil were not inferior to tamsulosin in terms of the USS using Bayesian analyses. In the random effect model, P-score tests showed that solifenacin possessed the highest P-score (p = 0.8484); tamsulosin was the second highest (p = 0.7054). As a result of the rank-probability test, solifenacin was also ranked highest in terms of USS, and tamsulosin was ranked second. Conclusions: Compared with the placebo, solifenacin, tamsulosin, and alfuzosin significantly decreased the USS. In our study, solifenacin may be considered the most effective medication for SRD.
Processing of Eddy Current Infrared Thermography and Magneto-Optical Imaging for Detecting Laser Welding Defects
Authors: Gao Pengyu , Yan Xin , He Jinpeng , Yang Haojun , Chen Xindu , Gao Xiangdong
Published: January 2025

Infrared (IR) magneto-optical (MO) bi-imaging is an innovative method for detecting weld defects, and it is important to process both IR thermography and MO imaging characteristics of weld defects. IR thermography and MO imaging can not only run simultaneously but can also run separately in special welding processes. This paper studies the sensing processing of eddy current IR thermography and MO imaging for detecting weld defects of laser spot welding and butt joint laser welding, respectively. To address the issues of high-level noise and low contrast in eddy current IR detection thermal images interfering with defect detection and recognition, a method based on least squares and Gaussian-adaptive bilateral filtering is proposed for denoising eddy current IR detection thermal images of laser spot welding cracks and improving the quality of eddy current IR detection thermal images. Meanwhile, the image gradient is processed by Gaussian-adaptive bilateral filtering, and then the filter is embedded in the least squares model to smooth and denoise the image while preserving defect information. Additionally, MO imaging for butt joint laser welding defects is researched. For the acquired MO images of welding cracks, pits, incomplete fusions, burn-outs, and weld bumps, the MO image processing method that includes median filtering, histogram equalization, and Wiener filtering was used, which could eliminate the noise in an image, enhance its contrast, and highlight the weld defect features. The experimental results show that the proposed image processing method can eliminate most of the noise while retaining the weld defect features, and the contrast between the welding defect area and the normal area is greatly improved. The denoising effect using the Natural Image Quality Evaluator (NIQE) and the Blind Image Quality Index (BIQI) has been evaluated, further demonstrating the effectiveness of the proposed method. The differences among weld defects could be obtained by analyzing the gray values of the weld defect MO images, which reflect the weld defect information. The MO imaging method can be used to investigate the magnetic distribution characteristics of welding defects, and its effectiveness has been verified by detecting various butt joint laser welding weldments.
Multi-Channel Electrical Discharge Machining of Ti-6Al-4V Enabled by Semiconductor Potential Differences
Authors: Zhu Xuyang , Wei Tao , Li Sipei , Li Guangxian , Ding Songlin
Published: January 2025

Titanium alloys are difficult to machine using conventional metal cutting methods due to their low thermal conductivity and high chemical reactivity. This study explores the new multi-channel discharge machining of Ti-6Al-4V using silicon electrodes, leveraging their internal resistivity to generate potential differences for multi-channel discharges. To investigate the underlying machining mechanism, the equivalent circuit model was developed and a theoretical simulation was carried out. Comparative experiments with silicon and conventional copper electrodes under identical parameters were also conducted to analyze discharge waveforms, material removal rate, surface quality, and heat-affected zones (HAZ). The results demonstrate that the bulk resistance of silicon is the main mechanism for generating multi-channel discharges. This process efficiently disperses the discharge energy of the single discharge pulse, resulting in smaller craters, smoother machined surfaces, and shallower recast layers and HAZ.
Wastewater-Based Epidemiological Surveillance in France: The SUM’EAU Network
Authors: Jourdain Frédéric , Toro Laila , Senta-Loÿs Zoé , Deryene Marilyne , Mokni Walid , Azevedo Da Graça Tess , Le Strat Yann , Rahali Sofiane , Yamada Ami , Maisa Anna , Pretet Maël , Sudour Jeanne , Cordevant Christophe , Chesnot Thierry , Roman Veronica , Wilhelm Amandine , Gassilloud Benoît , Mouly Damien
Published: January 2025

Wastewater surveillance is a powerful public health tool which gained global prominence during the COVID-19 pandemic. This article describes the development and implementation of the national wastewater surveillance network in France: SUM’EAU. Preliminary work included defining a sampling strategy, evaluating/optimising analytical methods, launching a call for tenders to select network laboratories and producing wastewater monitoring indicators. SUM’EAU was then deployed in three stages: (i) a pilot study, (ii) the transfer of analytical activities from the National Reference Laboratory to four selected network laboratories, and (iii) the extension of the system to additional sampling sites. Currently, SUM’EAU monitors SARS-CoV-2 across 54 wastewater treatment plants in mainland France. Once a week on business days, 24 h flow-proportional composite samples are collected at plant inlets and transported at 5 °C (±3 °C) to partner laboratories for analysis. The analytical process involves sample concentration, RNA extraction, and digital RT-PCR/q-RT-PCR to detect and quantify the presence of the SARS-CoV-2 genome in wastewater. Subsequently, data are transferred to Santé publique France, the French National Public Health Agency, for analysis and interpretation. While SUM’EAU has been instrumental in monitoring the COVID-19 pandemic and holds significant potential for broader application, securing sustainable funding for its operation remains a major challenge.
CO2 Capture: A Comprehensive Review and Bibliometric Analysis of Scalable Materials and Sustainable Solutions
Authors: Carrascal-Hernández Domingo Cesar , Grande-Tovar Carlos David , Mendez-Lopez Maximiliano , Insuasty Daniel , García-Freites Samira , Sanjuan Marco , Márquez Edgar
Published: January 2025

The greenhouse effect and global warming, driven by the accumulation of pollutants, such as sulfur oxides (SOx), nitrogen oxides (NOx), and CO2, are primarily caused by the combustion of fossil fuels and volcanic eruptions. These phenomena represent an international crisis that negatively impacts human health and the environment. Several studies have reported novel carbon capture, utilization, and storage (CCUS) technologies, promising solutions. Notable methods include chemical absorption using solvents, and the development of functionalized porous materials, such as MCM-41, impregnated with amines like polyethyleneimine. These technologies have demonstrated high capture capacity and thermal stability; however, they face challenges related to recyclability and high operating costs. In parallel, biodegradable polymers and hydrogels present sustainable alternatives with a lower environmental impact, although their industrial scalability remains limited. This review comprehensively analyzes CO2 capture methods, focusing on silica-based porous supports, polymers, hydrogels, and emerging techniques, like CCUS and MOFs, while including traditional methods and a bibliometric analysis to update the field’s scientific dynamics. With increasing investigations focused on developing new CCUS technologies, this study highlights a growing interest in eco-friendly alternatives. A bibliometric analysis of 903 articles published between 2010 and 2024 provides an overview of current research on environmentally friendly carbon capture technologies. Countries such as the United States, the United Kingdom, and India are leading research efforts in this field, emphasizing the importance of scientific collaboration. Despite these advancements, implementing these technologies in industrial sectors with high greenhouse gas emissions remains scarce. This underscores the need for public policies and financing to promote their development and application in these sectors. Future research should prioritize materials with high capture capacity, efficient transformation, and valorization of CO2 while promoting circular economy approaches and decarbonizing challenging sectors, such as energy and transportation. Integrating environmentally friendly materials, energy optimization, and sustainable strategies is essential to position these technologies as key tools in the fight against climate change.
Biomass-Based Hydrogen Extraction and Accompanying Hazards—Review
Authors: Nieścioruk Mariusz J. , Bandrow Paulina , Szufa Szymon , Woźniak Marek , Siczek Krzysztof
Published: January 2025

Nowadays, there is an increased demand for energy, the access to which, however, is limited due to the decreasing of fossil sources and the need to reduce emissions, especially carbon dioxide. One possible remedy for this situation is using hydrogen as a source of green energy. Hydrogen is usually bound to other chemical elements and can be separated via energy-intensive few-step conversion processes. A few methods are involved in separating H2 from biomass, including biological and thermochemical (TC) ones. Such methods and possible hazards related to them are reviewed in this study.
Aggregachromic Fluorogenic Asymmetric Cyanine Probes for Sensitive Detection of Heparin and Protamine
Authors: Kostadinov Anton , Vasilev Aleksey , Baluschev Stanislav , Landfester Katharina
Published: January 2025

The precise and fast detection of heparin, the most widely used anticoagulant, remains a significant challenge for assessing its use in a clinical setting. In this work, we adapt a well-established asymmetric cyanine fluorogenic platform for the purpose of ultrasensitive heparin detection in the presence of common interferant chemical species. Three analogous fluorescence probes are synthesized in order to optimize for the number of binding moieties. Their interaction with heparin is studied using steady-state absorption, fluorescence, and circular dichroism spectroscopy. The obtained probes exhibit a highly sensitive “turn-on” fluorescence response to heparin, with a LOD in the 10–25 nM range, well within practical requirement, as well as a visible colorimetric change. The heparin–probe complex is also employed as a sensitive detection platform for protamine, both in the “turn-off” fluorescence and ratiometric detection schemes.
Electrokinetics of CO2 Reduction in Imidazole Medium Using RuO2.SnO2-Immobilized Glassy Carbon Electrode
Authors: Rahaman Mostafizur , Islam Md. Fahamidul , Moushumy Zannatul Mumtarin , Hossain Md Mosaraf , Islam Md. Nurnobi , Hasan Mahmudul , Rahman Mohammad Atiqur , Tanjila Nahida Akter , Hasnat Mohammad A.
Published: January 2025

The pursuit of electrochemical carbon dioxide reduction reaction (CO2RR) as a means of energy generation and mitigation of global warming is of considerable interest. In this study, a novel RuO2-incorporated SnO2-fabricated glassy carbon electrode (GCE) with a Nafion binder was used for the electrochemical reduction of CO2 in an aqueous alkaline imidazole medium. The electrode fabrication process involved the drop-casting method, where RuO2.SnO2 was incorporated onto the surface of the GCE. Electrochemical studies demonstrated that the GCE-RuO2.SnO2 electrode facilitated CO2 reduction at −0.58 V vs. the reversible hydrogen electrode (RHE) via a diffusion-controlled pathway with the transfer of two electrons. Importantly, the first electron transfer step was identified as the rate-determining step (RDS). A Tafel slope of 144 mV dec−1 confirmed the association of two-electron transfer kinetics with CO2RR. Moreover, the standard rate constant (ko) and formal potential (E°′) were evaluated as 2.89 × 10−5 cm s−1 and 0.0998 V vs. RHE, respectively. Kinetic investigations also reveal that the deprotonation and electron release steps took place simultaneously in the CO2RR. Based on the reported results, the GCE-RuO2.SnO2 electrode could be a promising candidate for CO2 reduction, applicable in renewable energy generation.
Synthesis and Performance of Epoxy-Terminated Hyperbranched Polymers Based on Epoxidized Soybean Oil
Authors: Li Guang-Zhao , Wang Qiuhong , Zhu Chongyu , Zhang Shuai , Wang Fumei , Tao Lei , Jiang Youqi , Zhang Qiang , Wang Wenyan , Han Rui
Published: January 2025

Epoxy-terminated hyperbranched polymers (EHBPs) are a class of macromolecular polymers with a hyperbranched structure containing epoxy groups. They possess characteristics such as low viscosity, high functionality, and thermal stability, which endow them with broad application potential in materials science and chemical engineering. This study uses epoxidized soybean oil (ESO) as the raw material, which undergoes ring-opening reactions with glycerol and is esterified with 2,2-bis(hydroxymethyl)propionic acid (DMPA) to obtain epoxy soybean oil polyol (EGD) with a high hydroxyl value. Subsequently, four types of EHBPs are synthesized by incorporating epichlorohydrin (ECH) in mass ratios of 1:3, 1:4, 1:5, and 1:6 under strong alkaline conditions. The product structure is characterized using FT–IR and GPC. The degree of branching of EGD is calculated using 1H NMR and 13C NMR spectroscopy. The epoxy value of EHBPs is tested using the hydrochloric acid–acetone method, and the water contact angle, adhesion properties, rheological properties, and thermal properties of the EHBPs are also evaluated. The results show that the degree of branching of EGD is 0.45. The epoxy values of the EHBPs are 0.73, 0.79, 0.82, and 0.89 mol/100g, respectively. As the epoxy value and molecular weight of the epoxy hyperbranched polymers (EHBPs) increase, the water contact angle and adhesion strength of the EHBPs rise progressively and the viscosity decreases. Additionally, the glass transition temperature increases with the increase in the epoxy value. These epoxy hyperbranched polymers with low viscosity and high adhesion strength offer a promising approach for modifying surface coatings or formulating adhesives.
Molecular Dynamics-Assisted Discovery of Novel Phosphodiesterase-5 Inhibitors Targeting a Unique Allosteric Pocket
Authors: Luo Weihao , Liu Runduo , Cai Xinlin , Zhou Qian , Zhang Chen
Published: January 2025

Phosphodiesterase-5 (PDE5) is a potent therapeutic target for the treatment of male erectile dysfunction and pulmonary arterial hypertension with several drugs available on the market. However, most of the reported PDE5 inhibitors lack specificity over PDE6, a holoenzyme in eleven PDE families, which may cause various adverse effects. Targeting a unique allosteric pocket has proved to be an effective approach to designing selective PDE5 inhibitors. In the present study, an integrated virtual screening procedure consisting of pharmacophore modeling screening, molecular docking, molecular dynamics simulations, and binding free energy calculations was applied to the discovery of novel PDE5 inhibitors targeting the allosteric pocket. Seven out of thirty-three molecules purchased from the SPECS database (a hitting accuracy of 21%) with novel scaffolds were PDE5 inhibitors with enzymatic inhibition ratios of more than 50% at a concentration of 10 μM. Predicted binding patterns indicate these hits fit well in the allosteric pocket in PDE5. In particular, compound AI-898/12177002 (IC50 = 1.6 μM) demonstrates over 10-fold selectivity towards PDE6, providing a novel scaffold for the optimization of potent and selective PDE5 inhibitors with less adverse effects.
Silver Nanoparticles as Antimicrobial Agents in Veterinary Medicine: Current Applications and Future Perspectives
Authors: Frippiat Thibault , Art Tatiana , Delguste Catherine
Published: January 2025

Silver nanoparticles (AgNPs) have gained significant attention in veterinary medicine due to their antimicrobial properties and potential therapeutic applications. Silver has long been recognized for its ability to combat a wide range of pathogens, and when engineered at the nanoscale, silver’s surface area and reactivity are greatly enhanced, making it highly effective against bacteria, viruses, and fungi. This narrative review aimed to summarize the evidence on the antimicrobial properties of AgNPs and their current and potential clinical applications in veterinary medicine. The antimicrobial action of AgNPs involves several mechanisms, including, among others, the release of silver ions, disruption of cell membranes and envelopes, induction of oxidative stress, inhibition of pathogens’ replication, and DNA damage. Their size, shape, surface charge, and concentration influence their efficacy against bacteria, viruses, and fungi. As a result, the use of AgNPs has been explored in animals for infection prevention and treatment in some areas, such as wound care, coating of surgical implants, animal reproduction, and airway infections. They have also shown promise in preventing biofilm formation, a major challenge in treating chronic bacterial infections. Additionally, AgNPs have been studied for their potential use in animal feed as a supplement to enhance animal health and growth. Research suggested that AgNPs could stimulate immune responses and improve the gut microbiota of livestock, potentially reducing the need for antibiotics in animal husbandry. Despite their promising applications, further research is necessary to fully understand the safety, efficacy, and long-term effects of AgNPs on animals, humans, and the environment.
Effects of Frozen Red Dragon Fruit Consumption on Metabolic Markers in Healthy Subjects and Individuals at Risk of Type 2 Diabetes
Authors: Flores-Verastegui Mildred Inna Marcela , Coe Shelly , Tammam Jonathan , Almahjoubi Haythim , Bridle Robyn , Bi Sabina , Thondre Pariyarath Sangeetha
Published: January 2025

Background/Objectives: The interest in creating new products to decrease the risk of developing non-communicable chronic diseases such as type 2 diabetes (T2D) is increasing. These products include traditional food sources used as part of diverse cultures around the world, such as dragon fruit. The aim of this study was to investigate the effects of a frozen red dragon fruit (FRDF) beverage on blood pressure, glycaemic response (GR) and insulinaemic response (IR), lipid profile (LP), total antioxidant status (TAS), and C-reactive protein (CRP) levels in healthy subjects and individuals at risk of T2D. Methods: A parallel design trial (UREC registration number 211527; ClinicalTrials.gov registration number NCT05199636/19 January 2022) lasting four weeks and involving three testing sessions was conducted; participants were randomly assigned to one of two treatments (following general health guidance or consuming FRDF beverage). Systolic and diastolic blood pressures were taken; venous blood samples were collected to determine the LP and CRP levels; and capillary blood samples were taken before and after consuming a standard glucose drink to evaluate GR and IR at 15 min intervals (first hour) and 30 min intervals (second hour). Results: Eighteen participants completed this study, nine healthy (28.44 ± 5.20 years) and nine at risk (31.78 ± 12.11 years). The daily consumption of an FRDF-based beverage for four weeks by individuals at risk of T2D resulted in a reduction in blood pressure and IR–incremental area under the curve. The LP showed a downward trend, and a significant difference between treatments (p = 0.009) was found for CRP levels. Conclusions: Beverages based on FRDF may have the potential to decrease the risk of T2D.
Lipidomics Analysis Reveals the Effects of Docosahexaenoic Acid from Different Sources on Prefrontal-Cortex Synaptic Plasticity
Authors: He Zude , Xiong Wei , Yang Yue , Zhang Yifan , Li Boying , Wang Fuqing , Li Yixuan , Wang Ran , Sun Yanan
Published: January 2025

Background: Docosahexaenoic Acid (DHA) is an extensively used nutrition supplement in dairy food because of its beneficial effects on cognition. To find an effective DHA intervention for the synapses in the cortex during this period, this study aimed to use targeted lipidomics to evaluate the lipid composition of prefrontal-cortex (PFC) tissue in different DHA interference methods. Methods: Analyzed samples were taken from interfering feeding Bama pigs (BPs) (3 months) fed with soybean oil (Group B), blended oil (Group M), naturally DHA-supplemented milk with blended oil (Group OM), and DHA from fish oil (FO) with blended oil (Group Y). We also examined the protein expression levels of BDNF, GAP43, and MBP. Results: The lipidomics analysis identified 80 different related negative-ion lipid content and filtered the biomarker lipids in PFC tissue. We observed significant lipid composition changes between group Y and other groups, especially for content levels of phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylinositol (PI), phosphatidylserine (PS), and sphingomyelin (SM). The same observations were made from mRNA and protein expressions related to lipid transportation, phosphatidylserine (PS) synthetase, and synaptic plasticity in PFC tissues between group Y and other groups, including the mRNA expression levels of CD36, BDNF, and PTDSS1. The analysis of protein expression levels showed that the metabolism mode of DHA intervention from FO benefited the PFC, PS metabolism, and PFC synaptic plasticity of infants. Conclusions: The results highlight further prospects for the DHA intervention mode, which provides new routes for other studies on polyunsaturated-fatty-acid (PUFA) interference for infants.
Relating Workaholism to Job Stress: Serial Mediating Role of Job Satisfaction and Psychological Capital of Nurses in Angola
Authors: Geremias Rosa Lutete
Published: January 2025

Background/Objectives: Previous studies conducted in sub-Saharan African countries have concentrated on examining the challenges of nursing training and the organizational commitment of healthcare professionals, with little attention paid to exploring the mechanisms that contribute to reducing nurses’ job stress. Consequently, the present study addresses a significant gap in the literature by offering an overview of the factors contributing to understanding job stress among nurses in Angola. This study aimed to analyze the direct and indirect relationship between workaholism and job stress with job satisfaction and psychological capital mediating this relationship. Methods: Using the quantitative methodology with a cross-sectional design, a questionnaire was administered to 340 nurses (172 men and 168 women). Results: The results confirmed that workaholism is positively related to job stress and that job satisfaction and psychological capital serially mediate the relationship between workaholism and job stress. Conclusions: These findings highlight the importance of fostering job satisfaction and psychological capital by establishing favorable work environments and promoting nurses’ physical and emotional well-being. In addition, these results may encourage healthcare leaders to create well-designed break areas for nurses to take restorative breaks.
Promoting Self-Efficacy of Nursing Students in Academic Integrity Through a Digital Serious Game: A Pre/Post-Test Study
Authors: Creighton Laura , Brown Wilson Christine , Anderson Tara , Hamilton Conor , Curtis Guy , Slade Christine , Mitchell Gary
Published: January 2025

Background: Academic integrity is an important component of nursing education, bridging academic ethics with professional practice. This study evaluated the effectiveness of a co-designed Academic Integrity digital serious game in improving nursing students’ self-efficacy related to academic integrity, academic offenses, professionalism, and artificial intelligence use. Methods: A pre-test/post-test design was employed, using a bespoke questionnaire to assess 303 first-year nursing students’ self-efficacy before and after playing the game. The questionnaire covered five subscales: academic integrity standards, academic offenses, professional values, feedback processes, and AI use in academic work. Results: Statistically significant improvements were observed across all subscales following the intervention, indicating enhanced self-efficacy in understanding and applying academic integrity principles, recognizing academic offenses, demonstrating professional behaviors, utilizing feedback, and appropriately using AI in academic contexts. Conclusions: The Academic Integrity digital serious game has the potential to be an effective tool for enhancing nursing students’ self-efficacy in the areas of academic and professional ethics. This approach shows promise for integrating academic integrity-based education in nursing curricula and preparing students for the ethical challenges of modern healthcare practice. This study was not registered.
Duplication of a Type-P5B-ATPase in Laverania and Avian Malaria Parasites and Implications About the Evolution of Plasmodium
Authors: Wiser Mark F.
Published: January 2025

Two related P-type ATPases, designated as ATPase1 and ATPase3, were identified in Plasmodium falciparum. These two ATPases exhibit very similar gene and protein structures and are most similar to P5B-ATPases. There are some differences in the predicted substrate-binding sites of ATPase1 and ATPase3 that suggest different functions for these two ATPases. Orthologues of ATPase3 were identified in all Plasmodium species, including the related Hepatocystis and Haemoproteus. ATPase3 orthologues could also be identified in all apicomplexan species, but no clear orthologues were identified outside of the Apicomplexa. In contrast, ATPase1 orthologues were only found in the Laverania, avian Plasmodium species, and Haemoproteus. ATPase1 likely arose from a duplication of the ATPase3 gene early in the evolution of malaria parasites. These results support a model in which early malaria parasites split into two clades. One clade consists of mammalian malaria parasites and Hepatocystis but excludes P. falciparum and related Laverania. The other clade includes Haemoproteus, avian Plasmodium species, and Laverania. This contrasts to recent models that suggest all mammalian malaria parasites form a monophyletic group, and all avian malaria parasites form a separate monophyletic group. ATPase1 may be a useful taxonomic/phylogenetic character for the phylogeny of Haemosporidia.
Evaluation of the Dose of African Swine Fever Virus Required to Establish Infection in Pigs Following Oral Uptake
Authors: Olesen Ann Sofie , Lazov Christina Marie , Accensi Francesc , Johnston Camille Melissa , Rasmussen Thomas Bruun , Bøtner Anette , Lohse Louise , Belsham Graham J.
Published: January 2025

African swine fever virus (ASFV) is known to be very stable within a protein-rich environment and indirect virus transmission can be mediated via oral uptake of different materials. However, experimental studies in pigs have shown that infection by ASFV via the oral route can be difficult to establish. Currently, there is a lack of studies using strict oral inoculations of pigs with different doses of ASFV. Therefore, we aimed to determine the dose of a European genotype II ASFV that is required to establish infection of pigs by the oral route. In this study, 24 pigs were divided into four groups of six. Three of the groups were fed with a low, medium or high dose of the ASFV POL/2015/Podlaskie virus. The pigs in the fourth group served as positive controls and were inoculated intranasally, just once, using the low dose of the virus. All the pigs inoculated intranasally with ASFV succumbed to the infection, while only three of the six pigs that were fed the high dose of the virus became infected. None of the 12 pigs that were fed with either the medium or low dose of the virus became infected, despite receiving up to thirteen doses each. In two of the pigs infected by intranasal inoculation, the presence of a variant form of the ASFV genome was detected. The results obtained in this study underline that ASFV infection is more difficult to establish via the oral route when compared to the intranasal route. The high dose needed in order to establish oral infection could have implications for future strategies using baited vaccines containing infectious live-attenuated ASFV.
Aggregation-Caused Quenching Dyes as Potent Tools to Track the Integrity of Antitumor Nanocarriers: A Mini-Review
Authors: Wang Xiye , Huang Jiayue , Guo Mengqin , Zhong Yiling , Huang Zhengwei
Published: January 2025

Cancer has become one of the major causes of death worldwide. Chemotherapy remains a cornerstone of cancer treatment. To enhance the tumor-targeting efficiency of chemotherapy agents, pharmaceutical scientists have developed nanocarriers. However, the in vivo structural integrity and dynamic changes in nanocarriers after administration are not well understood, which may significantly impact their tumor-targeting abilities. In this paper, we propose the use of environmentally responsive fluorescent probes to track the integrity of antitumor nanocarriers. We compare three main types of dyes: fluorescence resonance energy transfer (FRET) dyes, aggregation-induced emission (AIE) dyes, and aggregation-caused quenching (ACQ) dyes. Among them, ACQ dyes, possessing sensitive water-quenching properties and easily detected “on–off” switching behavior, are regarded as the most promising choice. We believe that ACQ dyes are suitable for investigating the in vivo fate of antitumor nanocarriers and can aid in designing improved nanoformulations for chemotherapy agents.
Three-Dimensional Scalar Time-Dependent Photorefractive Beam Propagation Model
Authors: Cronin-Golomb Mark
Published: January 2025

This paper presents an open-source time-dependent three-dimensional scalar photorefractive beam propagation model (PRProp3D) based on the well-known split-step method. The angular spectrum method is used for the diffractive steps, and the nonlinearities accumulated at the end of each diffractive step are applied using spatially varying phase screens. Comparisons with previously published experimental results are given for image amplification, photorefractive amplified scattering (fanning) and photorefractive screening solitons. Artifacts can be mitigated by use of step sizes less than 5~10 micrometers and by careful choice of the transverse computation grid size to ensure adequate sampling. Wraparound effects associated with the use of discrete Fourier transforms are mitigated by apodization and beam centering.
Correlated Photon Lidar Based on Time-Division Multiplexing
Authors: Jiang Yun , Liu Bo , Wang Zixun , Huang Fengyun , Liu Taibei , Luo Lan , He Feifan , Yang Yongqi , Zhao Bin
Published: January 2025

Single-photon lidar (SPL) exhibits high sensitivity, making it particularly suitable for detecting weak echoes over long distances. However, its susceptibility to background noise necessitates the implementation of advanced filtering techniques and complex algorithms, which can significantly increase system cost and complexity. To address these challenges, we propose a time-division-multiplexing-based correlated photon lidar system that employs a narrowband pulsed laser with stable time delays and variable pulse intensities, thereby establishing temporal and intensity correlations. This all-fiber solution not only simplifies the system architecture but also enhances operational efficiency. An adaptive cross-correlation method incorporating time slicing has been developed to extract histogram signals, enabling successful 1.5 km distance measurements under intense daytime noise conditions, using a 1 s accumulation time and a 20 mm receiving aperture. The experimental results demonstrate a 38% (from 1.11 to 1.52) improvement in the signal-to-noise ratio (SNR), thereby enhancing the system’s anti-noise capability, facilitating rapid detection, and reducing overall system costs.
Synergistic Effects of Biochar and Irrigation on Sugar Beet Growth, Yield, Quality, and Economic Benefit in Arid Regions
Authors: Jiang Fuchang , Li Yi , Li Liwei , Xie Xiangwen , Xu Wanli , Gao Yang , Biswas Asim
Published: January 2025

Water scarcity hinders sustainable agriculture in arid and semi-arid regions. This study investigated the combined effects of trickle irrigation and biochar application on sugar beet cultivation in northwest China’s arid and semi-arid regions, addressing challenges of water scarcity. Three-year field experiments were conducted using plastic film mulch, four irrigation levels based on crop evapotranspiration (0.6–1.2 ETc), and four biochar application rates (0–30 t ha−1). Results showed that biochar application increased sugar beet germination rates by 7.2–24.5% and enhanced relative chlorophyll content by 3.1–22.1%. Optimal combinations of irrigation and biochar significantly improved growth indices and yield, with maximum values observed under the highest irrigation (1.2 ETc) and 10 t ha−1 biochar treatment. However, the 1.0 ETc irrigation treatment with 10 t ha−1 biochar demonstrated superior water use efficiency (14.8% higher), sustainable yield index (1.1% higher), and economic benefits (1.4% higher) compared to the highest irrigation treatment. Considering growth, yield, quality, water use efficiency, sustainability, and economic factors, an irrigation level of 1.0 ETc combined with a biochar application rate of 10 t ha−1 is recommended for sugar beet cultivation in Xinjiang. This study provides valuable insights and practical strategies for water conservation, high yield, and quality improvement in sugar beet cultivation under arid and semi-arid conditions, contributing to sustainable agricultural practices in water-scarce regions.
The Establishment of a Highly Efficient In Vitro Regeneration System for Viburnum opulus L. ‘Roseum’
Authors: Ning Yajing , Dong Hao , Zhang Xinxin , Li Yanhua , Cui Chengpeng , Li Shujuan
Published: January 2025

Viburnum opulus L. ‘Roseum’ is a highly valuable ornamental plant for landscaping, but it has a long propagation cycle and low propagation coefficient. In this study, stem segments with axillary buds from Viburnum opulus L. ‘Roseum’ were used as explants. We systematically analyzed the use of sodium hypochlorite for the sterilization of explants, as well as the effects of different plant growth regulator combinations and concentrations on shoot bud induction, shoot proliferation, the rooting of tissue-cultured shoots, and the transplanting of the tissue-cultured shoots. A complete rapid propagation technology system for Viburnum opulus L. ‘Roseum’ was established. The results showed that a disinfection method using 75% ethanol for 30 s and soaking in 5% sodium hypochlorite for 5 min was the most suitable for disinfecting the stem segments of Viburnum opulus L. ‘Roseum’, which showed low contamination and a 73.33% survival rate. The ideal medium for primary bud induction was WPM (Woody Plant Basal Medium) + 2.0 mg·L−1 6-benzylaminopurine (6-BA) + 0.15 mg·L−1 indole-3-butyric acid solution (IBA) + 25 g·L−1 sucrose. The optimal medium for shoot proliferation was WPM + 1.0 mg·L−1 6-BA + 0.15 mg·L−1 IBA + 25 g·L−1 sucrose, achieving an induction rate of 7.17. For the rooting of tissue-cultured shoots, the most suitable formulation was 1/2 WPM + 0.3 mg·L−1 naphthaleneacetic acid (NAA) + 0.3 mg·L−1 activated charcoal (AC) + 25 g·L−1 sucrose, which induced robust and developed root systems. This study provides a technical basis for the establishment of a fast propagation system for the industrial production of Viburnum opulus L. ‘Roseum’.
Evolutionary Dynamics and Expression Divergence of the MADS-Box Gene Family During Recent Speciation of AA-Genome Oryza Species
Authors: Tian Jiaqi , Gao Lizhi
Published: January 2025

To investigate the evolutionary trajectory during the recent speciation of AA-genome Oryza species, we conducted a comprehensive analysis of the MADS-box gene family across eight Oryza species. We identified 1093 MADS-box genes in total and systematically examined their evolutionary history, gene family expansion, and expression divergence. Our results revealed that extensive lineage-specific expansions occurred in AA-genome Oryza species, which were primarily generated by proximal and tandem duplications, with a particularly notable episode in Type-I genes. Despite the significant expansion, Type-I genes were generally expressed at low levels or not expressed across various organs. In contrast, the expansion of Type-II genes was primarily observed in the AG, AGL12, SOC1, GGM13, and MIKC* subfamilies, which exhibited high levels of expression in reproductive organs such as panicles and stigmas. Additionally, we found species-specific gene expression in the two out-crossing wild rice species, Oryza rufipogon and Oryza longistaminata. Notably, a unique MADS-box gene in O. longistaminata exhibited high expression levels in rhizomes and stems, which may be associated with the species’ distinctive rhizomatous growth habit.
Potential of Trichoderma asperellum as a Growth Promoter in Hydroponic Lettuce Cultivated in a Floating-Root System
Authors: Gutiérrez-Chávez Aldo , Robles-Hernández Loreto , Guerrero Brenda I. , González-Franco Ana Cecilia , Medina-Pérez Gabriela , Acevedo-Barrera Angélica Anahí , Hernández-Huerta Jared
Published: January 2025

The genus Trichoderma is widely used in agriculture as a biological agent and biofertilizer, enhancing crop yield and quality. However, its use in hydroponic systems is limited. This study evaluated the potential of Trichoderma asperellum as a growth promoter for lettuce (Lactuca sativa L.) cv. Starfighter RZ in a floating-root hydroponic system (FHS). T. asperellum strains (TaMFP1 and TaMFP2) were isolated from soil and identified morphologically and molecularly. The experiment used a completely randomized design with the following four treatments (n = 4): root spraying with TaMFP1, TaMFP2, T. harzianum (Trichospore®), and uninoculated plants (control). After 30 days, morphological, biochemical, and quality parameters were analyzed. All Trichoderma treatments significantly increased plant height (19.0%), root length (25.7%), total fresh biomass (76.4%), total dry biomass (82.63%), and number of leaves (18.18%). The nitrate levels in leaves were unaffected by TaMFP1 and TaMFP2, while Trichospore® reduced the nitrate content by 24.94%. The foliar nitrogen content increased with specific treatments, though the phosphorus and magnesium levels decreased. Visual quality traits, including appearance and firmness, remained unchanged. T. asperellum strains TaMFP1 and TaMFP2 enhanced vegetative growth without compromising quality, demonstrating their potential as sustainable tools for hydroponic lettuce production in controlled environments.
Metabolic and Nutritional Responses of Contrasting Aluminium-Tolerant Banana Genotypes Under Al Stress
Authors: Wu Xinran , Khan Shahbaz , Qi Yucheng , Zhang Chuanling , Anwar Sumera , Yin Liyan , Huang Jiaquan
Published: January 2025

Aluminum (Al) toxicity is a major constraint to crop productivity in acidic soils, frequently encountered in banana-growing regions. This study investigates physiological and biochemical responses to Al stress in two Cavendish banana genotypes, Baodao and Baxi (Musa acuminata L.), which exhibit contrasting levels of Al tolerance. Banana plantlets were grown hydroponically under three AlCl3 concentrations (0, 100, and 500 μM) for 24, 48, and 72 h. Root elongation was progressively inhibited with increasing Al concentrations, with Baodao showing greater inhibition than Baxi. Al primarily accumulated in roots and displayed genotype-specific distribution patterns: Baodao concentrated more Al in root tips, suggesting lower exclusion efficiency. In contrast, Baxi, the Al-tolerant genotype, translocated Al from roots to shoots more effectively, indicating potential sequestration mechanisms in less sensitive tissues. Al stress influenced enzyme activities, with Baxi exhibiting higher phosphoenolpyruvate carboxylase and citrate synthase activities at 100 µM Al, while both genotypes showed similar reductions at 500 µM. Baodao experienced more pronounced reductions in H+-ATPase activity. At 100 µM Al, Baxi retained higher levels of key nutrients (P, Zn, Mg, Mn, Fe, K, and B) in essential tissues than Baodao. However, nutrient levels were reduced in both genotypes at 500 µM Al. These findings highlight Baxi’s superior resilience under Al stress, making it a suitable genotype for cultivation and breeding in acidic soils.
Pre-Planting and Planting Factors and Practices Affecting Urban Tree Growth: With a Special Focus on the Root System and Its Condition—A Review
Authors: Korbik Mateusz , Swoczyna Tatiana , Latocha Piotr
Published: January 2025

Trees in urban conditions struggle with many factors that reduce their growth. In many cases, newly planted trees do not survive to maturity. The trees are produced using various methods, the most popular of which are balled and burlapped (B&B) and container production. Different production methods have their cons, but in many cases, the most common problem is the root system condition—it is often poorly developed, with girdle roots, or the rootball is covered with excess soil. Deep structural roots, as this is the name of the problem related to the roots being located too deep in the soil during production or trees being placed too deep in the planting pith, have been noticed for several decades; nevertheless, they are still poorly understood. In many cases, the excess soil above the rootball is over 10 cm—such covering the rootball may lead to infection, weakening, or tree death. The problem of deep structural roots seems to be one of the most serious problems we face in the case of urban plantings. However, many other factors remain disputed—such as cutting the crowns of planted trees, removing burlap from a rootball, or planting smaller rather than larger trees. All these issues have not been resolved despite many years of study, and still require further investigation.
Periodicity of Fruit Cracking in Orange Fruit and Integrated Management Intervention
Authors: Shi Xingjian , Wen Mingxia , Dong Zhihao , Zhang Jiangzhou , Srivastava Anoop Kumar , Moussa Mohamed G. , Zhang Yueqiang
Published: January 2025

Fruit cracking in citrus is one of the most researched constraints in crop management. However, researchers are still clueless even today on how to curtail this important production loss through an integrated management system. Our study introduces a management strategy for fruit cracking in citrus by analyzing different production constraints. As many as 70 Bingtang orange (Citrus sinensis L. Osbeck cv. Bingtang) orchards in Xinping County were investigated to determine the intensity and periodicity of fruit cracking. The results indicated that citrus cracking was in a high incidence state during production in the past two years, accounting for 48.2–50.6% of fruit drop following the physiological premature drop period, particularly exacerbating in the year with irregular rainfall (from June to September). Among factors such as soil texture, soil fertility, and orchard management, the soil sand proportion, soil calcium, soil potassium, and soil magnesium content were the main factors contributing to the occurrence of fruit cracking, with contributions of 18.57%, 17.14%, 10.00%, and 8.75%, respectively. Fruit cracking was significantly positively correlated with soil magnesium content (0.802) and significantly negatively correlated with soil calcium (0.8007), potassium (0.7616), and soil sand proportion (0.7826). The integrated management treatment (organic fertilizer to improve soil + foliar nutrient supplementation) showed better control on fruit cracking by 9.34–65.25% and an increase in yield by 4.13–37.49%, respectively, compared to the supplementation of a single element in all orchards with different production and quality traits. Our findings could thus help citrus growers optimize cultivation techniques for quality citrus production under increasingly changing climatic conditions.
Cutting-Edge Sensor Design: MIP Nanoparticle-Functionalized Nanofibers for Gas-Phase Detection of Limonene in Predictive Agriculture
Authors: Molinari Fabricio Nicolàs , Marelli Marcello , Berretti Enrico , Serrecchia Simone , Coppola Roxana Elisabeth , De Cesare Fabrizio , Macagnano Antonella
Published: January 2025

As population growth and climate change intensify pressures on agriculture, innovative strategies are vital for ensuring food security, optimizing resources, and protecting the environment. This study introduces a novel approach to predictive agriculture by utilizing the unique properties of terpenes, specifically S(-)-limonene, emitted by plants under stress. Advanced sensors capable of detecting subtle limonene variations offer the potential for early stress diagnosis and precise crop interventions. This research marks a significant leap in sensor technology, introducing an innovative active sensing material that combines molecularly imprinted polymer (MIP) technology with electrospinning. S(-)-limonene-selective MIP nanoparticles, engineered using methacrylic acid (MAA) and ethylene glycol dimethacrylate (EGDMA), were synthesized with an average diameter of ~160 nm and integrated into polyvinylpyrrolidone (PVP) nanofibers reinforced with multiwall carbon nanotubes (MWCNTs). This design produced a conductive and highly responsive sensing layer. The sensor exhibited rapid stabilization (200 s), a detection limit (LOD) of 190 ppb, and a selectivity index of 73% against similar monoterpenes. Optimal performance was achieved at 55% relative humidity, highlighting environmental conditions’ importance. This pioneering use of polymeric MIP membranes in chemiresistive sensors for limonene detection opens new possibilities for monitoring VOCs, with applications in agricultural stress biomarkers, contaminant detection, and air quality monitoring, advancing precision agriculture and environmental protection.