bluuebunny/crossref_metadata_2025 huggingface dataset:
Chloroplast Microsatellite-Based High-Resolution Melting Analysis for Authentication and Discrimination of Ilex Species
Authors: Kim Yonguk , Oh Dool-Ri , Kim Yu-Jin , Oh Kyo-Nyeo , Bae Donghyuk
Published: October 2022

Ilex species are important sources of high-quality raw plant materials for the production of drugs and functional foods. The precise identification of different species within the Ilex genus would greatly facilitate authentication and certification as well as forest resource monitoring in plantations. Combining DNA barcoding with high-resolution melting (HRM) analysis represents a robust strategy for species discrimination, as demonstrated in recent DNA barcoding studies. Here, using concatenated and aligned complete chloroplast genomes of different Ilex species, we conducted a sliding window analysis to identify regions of high nucleotide diversity (Pi). We optimized and validated the utility of PCR-based HRM coupled with microsatellite markers to discriminate among the four Ilex species, Ilex integra Thunb., Ilex rotunda Thunb., Ilex cornuta Lindl. and Paxton, and Ilex x wandoensis C.F. Mill and M. Kim, from wild populations in southwestern Korea. The marker trnSUGA-psbZ produced clear melting patterns and distinct melting curve profiles for the four Ilex species using HRM analysis. We applied this protocol to commercially available Ilex accessions and consistently identified the correct species for all 15 accessions tested. Therefore, combining DNA barcoding with HRM analysis is a powerful method for identifying different species within the same genus, which could be used for quality control of raw materials in the functional food/medicinal plant industry.
Biodiagnostics of Resistance to the Copper (Cu) Pollution of Forest Soils at the Dry and Humid Subtropics in the Greater Caucasus Region
Authors: Kolesnikov Sergey , Kuzina Anna , Minnikova Tatiana , Ter-Misyakyants Tigran , Nevedomaya Elena , Akimenko Yulia , Trufanov Dmitry , Kazeev Kamil , Burachevskaya Marina , Minkina Tatiana , Shende Sudhir S. , Barakhov Anatoly
Published: October 2022

Forest ecosystems perform important forestry and ecological functions. However, mining and processing companies cause significant soil contamination by heavy metals, in particular, copper (Cu). The resistance of nine types and subtypes of forest soils of the dry and humid subtropics in the Greater Caucasus region to Cu contamination at concentrations of 100, 1000, and 10,000 mg/kg was evaluated for the first time following the most sensitive and informative biological (microbiological, biochemical, and phytotoxic) indicators via a laboratory simulation study. Contamination was simulated under laboratory conditions. The series of forest soils was established following their resistance to Cu pollution: brown leached soils (Haplic Cambisols Eutric) = brown typical soils (Haplic Cambisols Eutric) > brown carbonate soils (Haplic Cambisols Eutric) = sod-carbonate typical soils (Rendzic Leptosols Eutric) ≥ yellow soils (Albic Luvisols Abruptic) ≥ leached sod-carbonate soils (Rendzic Leptosols Eutric) > brown forest slightly unsaturated soils (Haplic Cambisols Eutric) > acid brown forest soils (Haplic Cambisols Eutric) > acid brown forest podzolized soils (Haplic Cambisols Eutric). Regional environmentally safe standards for the Cu content in forest soils of the dry and humid subtropics of the Greater Caucasus were proposed: for brown typical soils, brown leached soils, brown carbonate soils, brown forest slightly unsaturated soils, sod-carbonate typical soils, leached sod-carbonate soils, and yellow soils, the rMPC was 100 mg/kg; for acid brown forest soils and acid brown forest podzolized soils, the rMPC was 70 mg/kg.
An Alternative to Vermiculite: Composting on Tropical Islands Using Coral Sand to Enhance Nitrogen Retention during Ventilation
Authors: Cheng Peng , Jiang Liqun , Shan Rui , Fang Zhen , Ma Nianfang , Deng Lianwu , Lu Yaoquan , Tan Xiangping , Shen Weijun , Liu Rongrong
Published: October 2022

Reducing nitrogen loss during composting with forced ventilation was comprehensively investigated in this study. Coral sand was tailored in the co-composting in the co-composting of sludge and litters. The physicochemical results revealed that forced ventilation prolonged the thermophilic phase and accelerated the substrate decomposition. With the addition of 10% native coral sand, the amount of nitrogen loss decreased by 9.2% compared with the original group. The microbial community evaluation revealed that the effect of forced ventilation on colony abundance was significantly greater than that of adding coral sand. This study demonstrated that when composting on a tropical island, adding coral sand under forced ventilation was a viable solution for realizing sustainable development.
Latest Advances in Preservation Technology for Fresh Fruit and Vegetables
Authors: Jin Peng
Published: October 2022

Fruit and vegetables contain abundant nutrients, as well as dietary and health benefits, and economic value, but suffer from shorter shelf life, declining quality, and rapid deterioration after harvest [...]
Facile Synthesis of Molecularly Imprinted Ratiometric Fluorescence Sensor for Ciguatoxin P-CTX-3C Detection in Fish
Authors: Qi Zhenke , Xiang Cheng , Tian Xingguo , Xu Xiaoyan
Published: October 2022

Ciguatoxin (CTX) detection methods are essential due to the serious hazard that bioaccumulation in fish and transmission along the food chain poses to human health. We report the rapid and simple development of a dual-emitting, molecularly imprinted, ratiometric fluorescence sensor (MIPs@BCDs/RCDs@SiO2) to detect ciguatoxin P-CTX-3C with high sensitivity and selectivity. The sensor was fabricated via sol–gel polymerization using monensin as the fragmentary dummy template molecule, blue carbon dots (BCDs) as the response signal, and red carbon dots (RCDs) as the reference signal. The fluorescence emission of BCDs was selectively quenched in the presence of P-CTX-3C, leading to a favorable linear correlation between the fluorescence intensity ratio (I440/I675) and the P-CTX-3C concentration in the range of 0.001–1 ng/mL with a lower detection limit of 3.3 × 10−4 ng/mL. According to LC-MS measurement results, the proposed sensor can rapidly detect ciguatoxin P-CTX-3C in coral reef fish samples with satisfactory recoveries and standard deviations. This study provides a promising strategy for rapid trace analysis of marine toxins and other macromolecular pollutants in complex matrices.
Plasma-Activated Acidic Electrolyzed Water: A New Food Disinfectant for Bacterial Suspension and Biofilm
Authors: Heng Yaping , Wang Ming , Jiang Hongwei , Gao Shumin , Zhang Jin , Wan Jinlin , Song Tingji , Ren Zhandong , Zhu Yuchan
Published: October 2022

Food-borne diseases are widespread all over the world, and food safety has attracted much attention. This study is the first to use plasma to activate acidic electrolyzed water (AEW) to obtain a new disinfectant for food processing. The germicidal efficacy of plasma-activated acidic electrolyzed water (PA-AEW) on B. subtilis suspension and biofilm was investigated. Furthermore, the synergistic effect of different bactericidal factors was inferred by investigating the physicochemical parameters of PA-AEW and the influencing factors of bactericidal effect. The results demonstrate that PA-AEW is a highly effective and rapid disinfectant. The killing logarithm (KL) value of PA-AEW on B. subtilis suspension could reach 2.33 log10CFU/mL with a sterilization time of 10 s, which is significantly higher than that of AEW (KL = 0.58 log10CFU/mL) and plasma-activated water (PAW) (KL = 0.98 log10CFU/mL) (significant difference, p < 0.01). Moreover, the KL value of the B. subtilis biofilm of PA-AEW was 2.41 log10CFU/mL, better than that of PAW and AEW (significant difference, p < 0.01), indicating that PA-AEW has important application prospects in food processing. The synergistic effect should come from the interaction between reactive chlorine species (RCS) and reactive oxygen and nitrogen species (RONS) in PA-AEW.
Enhancing the Nutritional and Functional Properties of Auricularia auricula through the Exploitation of Walnut Branch Waste
Authors: Hao Zhenkun , Zhang Wen’e , Tian Fenghua , Wei Rong , Pan Xuejun
Published: October 2022

As the third most edible fungus in the world, Auricularia auricular needs a lot of sawdust for cultivation; thus, it is a win–win method to develop waste wood sawdust suitable for black agaric cultivation. This study evaluated the growth, agronomic characters and nutritional quality of A. auricula cultured on different ratios of miscellaneous sawdust and walnut waste wood sawdust, and comprehensively analyzed the feasibility of cultivating black agaric with walnut sawdust using principal component method (PCA). The results showed that the macro mineral elements and phenolic substances in walnut sawdust were significantly higher than those of miscellaneous sawdust by 18.32–89.00%. The overall activity of extracellular enzymes reached the highest when the ratio of the substrate was 0:4 (miscellaneous sawdust: walnut sawdust). The mycelia of 1:3 substrates grew well and fast. In addition, the growth cycle for A. auricula was significantly lower for 0:4 (116 d) than for 4:0 (126 d). Then, the single bag yield and biological efficiency (BE) were highest at 1:3. Moreover, the nutrients and mineral elements of A. auricula cultivated in walnut sawdust were significantly higher than that of miscellaneous sawdust, expect for total sugar and protein, and the highest overall value was found at 1:3. Finally, the results of comprehensive evaluation by PCA showed that the D value was the highest when the substrate was 1:3 and the lowest when the substrate was 4:0. Therefore, the substrate ratio of 1:3 was the most suitable for the growth of A. auricula. In this study, the high yield and quality of A. auricula were cultivated by waste walnut sawdust, which provided a new way to utilize walnut sawdust.
Extraction, Isolation and Nutritional Quality of Coffee Protein
Authors: Bhattarai Rewati Raman , Al-Ali Hayder , Johnson Stuart K.
Published: October 2022

Coffee protein is reported to have high levels of branched-chain amino acids of value in sports nutrition and malnutrition recovery. However, data demonstrating this unusual amino acid composition are limited. We investigated the extraction and isolation of protein concentrates from coffee bean fractions, viz. green coffee, roasted coffee, spent coffee and silver skin, and determined their amino acid profile, caffeine content and protein nutritional quality, polyphenol content and antioxidant activity. Alkaline extraction/isoelectric precipitation gave lower concentrate yields and protein content than alkaline extraction/ultrafiltration. The protein concentrate from green coffee beans had a higher protein content than those from roasted coffee, spent coffee and silver skin, regardless of extraction method. The isoelectric precipitated green coffee protein concentrate had the highest in vitro protein digestibility and in vitro protein digestibility corrected amino acid score (PDCAAS). Silver skin protein concentrate had a very low digestibility and in vitro PDCAAS. In contrast to a previous finding, the amino acid levels in all coffee concentrates did not demonstrate high levels of branched-chain amino acids. All protein concentrates had very high levels of polyphenols and high antioxidant activity. The study suggested investigating coffee protein’s techno-functional and sensory attributes to demonstrate their potential applications in different food matrices.
Effects of Ultrasound-Assisted Immersion Freezing on the Protein Structure, Physicochemical Properties and Muscle Quality of the Bay Scallop (Argopecten irradians) during Frozen Storage
Authors: Liu Bing , Liao You-Lin , Jiang Liang-Liang , Chen Miao-Miao , Yang Shan-Bin
Published: October 2022

In this study, the comparison effects of ultrasound-assisted immersion freezing (UIF) at different ultrasonic power, immersion freezing (IF), and air freezing (AF) on the protein thermal stability, protein structure, and physicochemical properties of adductor muscle of scallop (Argopecten irradians) (AMS) during frozen storage were investigated. Principal component analysis and the Taylor diagram were used to comprehensively analyze all the indicators tested. The results showed that the UIF at 150 W (UIF-150) treatment was the most effective way to delay the quality deterioration of AMS during 90-day frozen storage. This was mainly because, compared to AF and IF treatments, UIF-150 treatment more effectively minimized the changes in the primary, secondary and tertiary structures of myofibrillar proteins, and it preserved the protein thermal stability of AMS by producing small and regular ice crystals in the AMS tissue during the freezing process. Moreover, the results of physicochemical properties indicated that UIF-150 treatment significantly inhibited the fat oxidation and microbiological activities of frozen AMS, and it finally maintained the microstructure and texture of AMS during frozen storage. Overall, UIF-150 has potential industrial application prospects in the rapid freezing and quality preservation of scallops.
Palmatine Inhibits the Pathogenicity of Aeromonas hydrophila by Reducing Aerolysin Expression
Authors: Dong Jing , Yan Tianhui , Yang Qiuhong , Song Yi , Cheng Bo , Zhou Shun , Liu Yongtao , Ai Xiaohui
Published: October 2022

Aeromonas hydrophila, an opportunistic aquatic pathogen widely spread in aquatic environments, is responsible for a number of infectious diseases in freshwater aquaculture. In addition, A. hydrophila can transmit from diseased fish to humans and results in health problems. The occurrence of antibiotic-resistant bacterial strains restricts the application of antibiotics and is responsible for failure of the treatment. Moreover, residues of antibiotics in aquatic products often threaten the quality and safety. Therefore, alternative strategies are called to deal with infections caused by antibiotic-resistant bacteria. Aerolysin, one of the most important virulence factors of A. hydrophila, is adopted as a unique anti-virulence target on the basis of the anti-virulence strategy to battling infections caused by A. hydrophila. Palmatine, an isoquinoline alkaloid from a variety of herbal medicines that showed no anti-A. hydrophila activity, could reduce hemolysis of the bacterium by decreasing aerolysin production. The results of the qPCR assay demonstrated that the transcription of the aerA gene was suppressed. Moreover, cell viability and in vivo study showed that palmatine treatment could decrease the pathogenicity of A. hydrophila both in vitro and in vivo. In summary, palmatine is a leading compound against A. hydrophila-associated infection in aquaculture by inhibiting the expression of aerolysin.
Effects of Cysteine and Inorganic Sulfur Applications at Different Growth Stages on Grain Protein and End-Use Quality in Wheat
Authors: Cai Jian , Zang Fujuan , Xin Liang , Zhou Qin , Wang Xiao , Zhong Yingxin , Huang Mei , Dai Tingbo , Jiang Dong
Published: October 2022

The aim of this study was to test the significant effects of inorganic sulfur and cysteine on grain protein and flour quality in wheat and to provide a theoretical basis of wheat cultivation techniques with high yield and quality. In the field experiment, a winter wheat cultivar, Yangmai 16, was used, and five treatments were established, i.e., S0 (no sulfur fertilizer application during the whole wheat growth period), S(B)60 (60 kg ha−1 inorganic sulfur fertilizer was applied as the basal fertilizer), Cys(B)60 (60 kg ha−1 cysteine sulfur fertilizer was applied as the basal fertilizer), S(J)60 (60 kg ha−1 inorganic sulfur fertilizer was applied as the jointing fertilizer), and Cys(J)60 (60 kg ha−1 cysteine sulfur fertilizer was applied as the jointing fertilizer). The fertilizer application at jointing stage showed a better influence than basal fertilizer application on protein quality; for the content of albumin, gliadin, and high molecular weight glutenin (HMW-GS), Cys(J)60 was the best among these treatments. An increase of 7.9%, 24.4%, 43.5%, 22.7% and 36.4% was found in grain yield, glutenin content, glutenin macro-polymer (GMP), low molecular weight glutenin (LMW-GS), and S content under Cys(J)60, in relation to the control, respectively. A similar trend was found in the end-use quality, as exemplified by an increase of 38.6%, 10.9%, 60.5%, and 109.8% in wet gluten content, dry gluten content, sedimentation volume, and bread-specific volume, respectively; a decrease of 69.3% and 69.1% in bread hardness and bread chewiness was found under Cys(J)60. In terms of application period, topdressing at jointing stage is compared with base fertilizer, the sulfur fertilizer application at jointing stage showed larger effects on grain protein and flour quality, from the different types of sulfur fertilizer, the application of cysteine performed better than the use of inorganic sulfur. The Cys(J)60 exhibited the best effects on protein and flour quality. It was suggested that sufficient sulfur application at jointing stage has the potential to enhance the grain protein and flour quality.
A Bayesian Approach to Estimating Age from the Auricular Surface of the Ilium in Modern American Skeletal Samples
Authors: Hens Samantha M. , Godde Kanya
Published: October 2022

Age estimation from human skeletal remains is a critical component of the biological profile for unidentified decedents. Using a Bayesian approach, we examine two popular methods (Lovejoy–LJ, and Buckberry zand Chamberlain–BC) for estimating age from the auricular surface of the ilium. Ages of transition are generated from a modern Portuguese skeletal sample (n = 466) and are coupled with an informative prior from historic Spitalfields, London (n = 179) to estimate age in a sample of modern Americans from the Bass Donated collection (n = 639). The Bass collection was challenging to statistically model, potentially due to higher morbidity and mortality characteristics of the central southern United States. The highest posterior density ranges provide a realized accuracy between 84–89% for males and 85–91% for females using the LJ method, and a realized accuracy between 79–82% for males and 65–71% for females using the BC method. Both methods worked well for older individuals. Cumulative binomials showed that both methods significantly underperformed; however, results were better for the LJ method, which also showed lower bias. Reference tables for aging modern American samples are provided, and the data meet Daubert guidelines, i.e., legal criteria for acceptable scientific evidence in a court of law in the United States.
Towards a Holistic Perspective on Future Transportation Systems: A Swedish Case and a Conceptual Framework
Authors: Große Christine
Published: October 2022

Critical infrastructure systems—such as transportation—are the backbone of society. Infrastructure development can thus be acknowledged as a common societal concern in the field of governance, and its recognition as a policy problem is crucial to proper decision making. This study aims to build an in-depth understanding of the multi-level system concerned with transportation and applies a soft systems methodology to structure the investigation. The system analysis and conceptual modelling rests on publicly available documents and policies, group meetings and a workshop with stakeholders from the local, regional and national levels. The paper provides a thorough analysis of the Swedish transportation system through public policy and the perceptions of municipal officials. In addition, it proposes a novel conceptual framework of the transportation system, including a detailed discussion of stakeholders, activities and perspectives. Although transportation is vital for many stakeholders, improving only transport infrastructure does not guarantee regional growth. Therefore, the proposed framework constitutes a novel basis for constructive dialogue among concerned parties regarding improvements to transportation. Thereby, the paper provides an understanding of a society’s transportation system that can be used to inform agenda setting for critical infrastructure governance. Further discussion in the scientific community and with officials entrusted with public administration could further validate and deepen the proposed understanding of the perspectives and constraints in the examined context and beyond.
Host Genetic Risk Factors Associated with COVID-19 Susceptibility and Severity in Vietnamese
Authors: Nhung Vu Phuong , Ton Nguyen Dang , Ngoc Tran Thi Bich , Thuong Ma Thi Huyen , Hai Nguyen Thi Thanh , Oanh Kim Thi Phuong , Hien Le Thi Thu , Thach Pham Ngoc , Hai Nong Van , Ha Nguyen Hai
Published: October 2022

Since the emergence and rapid transmission of SARS-CoV-2, numerous scientific reports have searched for the association of host genetic variants with COVID-19, but the data are mostly acquired from Europe. In the current work, we explored the link between host genes (SARS-CoV-2 entry and immune system related to COVID-19 sensitivity/severity) and ABO blood types with COVID-19 from whole-exome data of 200 COVID-19 patients and 100 controls in Vietnam. The O blood type was found to be a protective factor that weakens the worst outcomes of infected individuals. For SARS-CoV-2 susceptibility, rs2229207 (TC genotype, allele C) and rs17860118 (allele T) of IFNAR2 increased the risk of infection, but rs139940581 (CT genotype, allele T) of SLC6A20 reduced virus sensitivity. For COVID-19 progress, the frequencies of rs4622692 (TG genotype) and rs1048610 (TC genotype) of ADAM17 were significantly higher in the moderate group than in the severe/fatal group. The variant rs12329760 (AA genotype) of TMPRSS2 was significantly associated with asymptomatic/mild symptoms. Additionally, rs2304255 (CT genotype, allele T) of TYK2 and rs2277735 (AG genotype) of DPP9 were associated with severe/fatal outcomes. Studies on different populations will give better insights into the pathogenesis, which is ethnic-dependent, and thus decipher the genetic factor’s contribution to mechanisms that predispose people to being more vulnerable to COVID-19.
RNA Modification-Related Genetic Variants in Genomic Loci Associated with Bone Mineral Density and Fracture
Authors: Han Limin , Wu Jingyun , Wang Mimi , Zhang Zhentao , Hua Dian , Lei Shufeng , Mo Xingbo
Published: October 2022

Genome-wide association studies (GWASs) have identified more than 500 loci for bone mineral density (BMD), but functional variants in these loci are less known. The aim of this study was to identify RNA modification-related SNPs (RNAm-SNPs) for BMD in GWAS loci. We evaluated the association of RNAm-SNPs with quantitative heel ultrasound BMD (eBMD) in 426,824 individuals, femoral neck (FN) and lumbar spine (LS) BMD in 32,961 individuals and fracture in ~1.2 million individuals. Furthermore, we performed functional enrichment, QTL and Mendelian randomization analyses to support the functionality of the identified RNAm-SNPs. We found 300 RNAm-SNPs significantly associated with BMD, including 249 m6A-, 28 m1A-, 3 m5C-, 7 m7G- and 13 A-to-I-related SNPs. m6A-SNPs in OP susceptibility genes, such as WNT4, WLS, SPTBN1, SEM1, FUBP3, LRP5 and JAG1, were identified and functional enrichment for m6A-SNPs in the eBMD GWAS dataset was detected. eQTL signals were found for nearly half of the identified RNAm-SNPs, and the affected gene expression was associated with BMD and fracture. The RNAm-SNPs were also associated with the plasma levels of proteins in cytokine-cytokine receptor interaction, PI3K-Akt signaling, NF-kappa B signaling and MAPK signaling pathways. Moreover, the plasma levels of proteins (CCL19, COL1A1, CTSB, EFNA5, IL19, INSR, KDR, LIFR, MET and PLXNB2) in these pathways were found to be associated with eBMD in Mendelian randomization analysis. This study identified functional variants and potential causal genes for BMD and fracture in GWAS loci and suggested that RNA modification may play an important role in osteoporosis.
The PPARGC1A Is the Gene Responsible for Thrifty Metabolism Related Metabolic Diseases: A Scoping Review
Authors: Aisyah Riandini , Sadewa Ahmad Hamim , Patria Suryono Yudha , Wahab Abdul
Published: October 2022

The “thrifty genotype” hypothesis has thus far described the relationship between specific genes and the population’s resilience to food scarcity circumstances, but its link to the widespread prevalence of genetic diseases and metabolic syndrome has not been adequately mapped. The purpose of the study was to discover genes responsible for thrifty metabolism. A systematic search with keywords was performed for relevant titles. This study used the article’s database published by Pubmed, Proquest, and EBSCO from January, 2009 to September, 2022. Out of 418 papers screened for eligibility, the final evaluation determined that five studies should be included in the analysis. Results indicated that PPARGC1A Gly482Ser led to high BMI in the Tongans population but was unrelated to the onset of type 2 diabetes mellitus, but this was not the case in the Maori population. Significantly differing frequencies of PPAR C1431T and Pro12Ala gene polymorphisms were observed in the Iranian population. GWAS identification of additional genes in Asian and European populations did not produce consistent findings. As a summary, PPARGC1A Gly482Ser addresses as the gene responsible for thrifty metabolism in the Pacific population although some studies show inconsistent results.
Cuneiform Tablets Micro-Surveying in an Optimized Photogrammetric Configuration
Authors: Antinozzi Sara , Fiorillo Fausta , Surdi Mirko
Published: October 2022

In the current panorama of 3D digital documentation, the survey of tiny artifacts with micrometric details is strongly influenced by two factors: firstly, the still high cost of the instruments and technologies (active sensors) required to achieve the necessary level of accuracy and resolution; secondly, the needed professional skills for the macro-photogrammetric approach. In this context, this research aims to meet the demand for a digital survey and 3D representation of small objects with complex surfaces and sub-millimeter morphological characteristics using a low-cost configuration (passive sensors) for an image-based approach. The experiments concerned cuneiform tablets, which are challenging due to their morphological and geometrical characteristics. The digital replica of these unique artefacts can be helpful for their study and interpretation and many innovative applications: access and sharing, a collaborative interdisciplinary study among several experts, experimentation with machine learning for automatic character recognition, and linguistic studies. The micrometric surveying system described proves to be an efficient and reliable solution for cuneiform tablet digitization and documentation.
Resisting Return to Dutch Colonial Rule: Political Upheaval after Japanese Surrender during the Independence Movement in Sulawesi, Indonesia
Authors: Nadjamuddin Lukman , Akbar Amar Ali , Perkasa Adrian , Wargadalem Farida R. , Lumangino Wilman D.
Published: October 2022

Central Sulawesi is a part of Indonesia with a fascinating history during the revolutionary period (1945–1950), owing to several important events related to Indonesian sovereignty. This study uses historical methods to examine the involvement of the Netherlands Indies Civil Administration and its effort to recolonize the area. The Malino Conference, which led to the formation of the State of East Indonesia, was intended to legitimize the federated state under Dutch control and reduce the territory of the Republic of Indonesia. The Central Sulawesi Indonesian People’s Struggle Party is a unification of political parties that consistently maintained Central Sulawesi as part of the Republic of Indonesia, strengthening its bargaining position with the Dutch. This situation brought strong pressure to bear upon the Netherlands to immediately recognize the sovereignty of the Republic of Indonesia.
New Insights into the Role of Alternating Temperatures and Cyanide in the ROS-Mediated Cardoon Seed Dormancy Termination
Authors: Puglia Giuseppe Diego , Balestrasse Karina , Bustos José Santiago , Huarte Héctor Roberto
Published: October 2022

Physiological dormancy in wild cardoon (Cynara cardunculus var. sylvestris) can be terminated by achenes exposure to alternating temperatures, likely with the participation of reactive oxygen species (ROS). Cyanide is a natural compound that mediates seed dormancy removal in some plant species in association with oxidative signalling exerted by ROS. To date, no study has been conducted on the cyanide effect on ROS homeostasis during the germination of cardoon. Here, we showed that the addition of cyanide at low concentrations in dormant cardoon achenes promotes dormancy breakage at a constant temperature, speeds up germination to alternating temperatures and promotes ROS accumulation in embryonic axes of dormant achenes. The in-silico transcriptome analysis showed that the expression levels of transcripts of genes associated with ROS signalling and production, calcium signalling, gibberellins biosynthesis and cell wall loosening were significantly up-regulated at the alternating temperatures imbibition condition. In contrast, the expression of gene transcripts associated with the inhibition of germination, ABA biosynthesis and signalling were up-regulated at the constant temperature imbibition. However, no significant difference in lipid peroxidation or protein carbonylation levels was observed when achenes were imbibed at constant or alternating temperature conditions. These results suggest that dormancy termination triggered by alternating temperatures or cyanide could be mediated by ROS production and signalling in the cardoon embryonic axis, but this does not determine extensive protein carbonylation.
Galvanic Skin Response Features in Psychiatry and Mental Disorders: A Narrative Review
Authors: Markiewicz Renata , Markiewicz-Gospodarek Agnieszka , Dobrowolska Beata
Published: October 2022

This narrative review is aimed at presenting the galvanic skin response (GSR) Biofeedback method and possibilities for its application in persons with mental disorders as a modern form of neurorehabilitation. In the treatment of mental disorders of various backgrounds and courses, attention is focused on methods that would combine pharmacological treatment with therapies improving functioning. Currently, the focus is on neuronal mechanisms which, being physiological markers, offer opportunities for correction of existing deficits. One such indicator is electrodermal activity (EDA), providing information about emotions, cognitive processes, and behavior, and thus, about the function of various brain regions. Measurement of the galvanic skin response (GSR), both skin conductance level (SCL) and skin conductance responses (SCR), is used in diagnostics and treatment of mental disorders, and the training method itself, based on GSR Biofeedback, allows for modulation of the emotional state depending on needs occurring. Summary: It is relatively probable that neurorehabilitation based on GSR-BF is a method worth noticing, which—in the future—can represent an interesting area of rehabilitation supplementing a comprehensive treatment for people with mental disorders.
Examining the Relationship between Death Anxiety and Well-Being of Frontline Medical Staff during the COVID-19 Pandemic
Authors: Zhao Na , Liu Beikun , Wang Yiheng
Published: October 2022

To examine the well-being of medical staff during the COVID-19 pandemic, we conducted a survey of 705 medical staff who were involved in anti-epidemic work in China from 20 February to 16 March 2020. The findings of the present study showed a “psychological typhoon eye” effect in which the medical staff in areas with a high contagion rate showed a significantly lower level of death anxiety than those in low-contagion regions. We also found a significant negative relationship between death anxiety and hedonic well-being, but there was no relationship between death anxiety and eudaimonic well-being. Moreover, the results revealed that a narcissistic personality moderates the relationships between death anxiety and the two types of well-being. For those who had higher narcissistic personality scores, death anxiety had no negative effect on their well-being. The findings of the present study can help us to better understand the life profiles of medical staff and can also provide some practical implications for understanding the life conditions of medical staff when facing a great health crisis.
A Decision-Theoretic Public Health Framework for Heated Tobacco and Nicotine Vaping Products
Authors: Levy David T. , Cadham Christopher J. , Li Yameng , Yuan Zhe , Liber Alex C. , Oh Hayoung , Travis Nargiz , Issabakhsh Mona , Sweanor David T. , Sánchez-Romero Luz Maria , Meza Rafael , Cummings K. Michael
Published: October 2022

Markets for nicotine vaping products (NVPs) and heated tobacco products (HTPs) have grown as these products became positioned as harm-reduction alternatives to combusted tobacco products. Herein, we present a public health decision-theoretic framework incorporating different patterns of HTP, NVP, and cigarette use to examine their impacts on population health. Our framework demonstrates that, for individuals who would have otherwise smoked, HTP use may provide public health benefits by enabling cessation or by discouraging smoking initiation and relapse. However, the benefits are reduced if more harmful HTP use replaces less harmful NVP use. HTP use may also negatively impact public health by encouraging smoking by otherwise non-smokers or by encouraging initiation or relapse into smoking. These patterns are directly influenced by industry behavior as well as public policy towards HTPs, NVPs, and cigarettes. While substantial research has been devoted to NVPs, much less is known about HTPs. Better information is needed to more precisely define the health risks of HTPs compared to cigarettes and NVPs, the relative appeal of HTPs to consumers, and the likelihood of later transitioning to smoking or quitting all products. While our analysis provides a framework for gaining that information, it also illustrates the complexities in distinguishing key factors.
Lung Cancer in the French West Indies: Role of Sugarcane Work and Other Occupational Exposures
Authors: Cabréra Léïla , Auguste Aviane , Michineau Léah , Joachim Clarisse , Deloumeaux Jacqueline , Luce Danièle
Published: October 2022

Objective: Our aim was to study the role of occupational exposures in lung cancer risk in the French West Indies, with special attention to some specific activities, such as sugarcane work, that can only be studied in a limited number of populations. Methods: We used data from a population-based case-control study that included 147 incident lung cancer cases and 405 controls. Smoking histories and detailed occupational histories with descriptions of tasks and substances were collected by questionnaire during face-to-face interviews. Odds ratios (OR) adjusted for sex, age, region, smoking status, and cigarette pack-years and 95% confidence intervals (95% CI) were estimated by unconditional logistic regression. Results: Significantly increased risks of lung cancer were found in sugarcane farm workers (OR = 2.7; 95% CI 1.1–6.6) and more generally in the sugarcane-growing sector (OR = 2.5; 95% CI 1.0–6.3) and to a lesser extent in rum production. Elevated risks of lung cancer were also observed among other agricultural workers, painters, warehouse porters, labourers, and maintenance and motor vehicle repair workers. Exposure to herbicides in sugarcane cultivation was associated with an increased risk of lung cancer (OR = 2.6; 95% CI 0.9–7.6). Conclusion: These results show that occupational exposures contributed to lung cancer risk in the French West Indies, and highlighted the role of exposures related to sugarcane work.
Examining the Associations between Post-Stroke Cognitive Function and Common Comorbid Conditions among Stroke Survivors
Authors: Morrison Helena W. , White Melissa M. , Rothers Janet L. , Taylor-Piliae Ruth E.
Published: October 2022

A considerable complication for stroke survivors is the subsequent development of cognitive decline or dementia. In this study, the relationship between the inflammation-centered comorbidity burden on post-stroke cognitive function among community-dwelling stroke survivors capable of independent living was examined. Data for this secondary analysis were collected from stroke survivors (n = 97) participating in a randomized clinical trial. Participants provided baseline responses, regarding cognitive function (mini-mental status exam, MMSE; Montreal cognitive assessment, MoCA), history of stroke comorbid conditions, and the Stroke Prognosis Instrument-II (SPI-II), an index of stroke comorbidity and recurrent stroke risk within the next two years. Relationships and differences between groups were tested for significance using Spearman’s correlation, Kruskal–Wallis, or Mann–Whitney U tests. Most stroke survivors (69%) had multiple comorbidities. Total SPI-II scores were negatively correlated to both MoCA and MMSE scores (r = −0.25, p = 0.01; r = −0.22, p = 0.03, respectively), and differences in MoCA scores among SPI-II risk groups (low, medium, high) were evident (p = 0.05). In contrast, there were no differences in MoCA or MMSE scores when comorbid conditions were examined individually. Lastly, no gender differences were evident in cognitive assessments. Our data support the premise that comorbidity’s burden impacts post-stroke cognitive decline, more than a single comorbid condition. Inflammation may be an important component of this comorbidity burden. Future studies that operationalize this concept will better illuminate the complex phenomenon of post-stroke cognitive decline for improved clinical rehabilitation modalities.
Tumour Hidden behind Thoracic Spine Pain: A Rare Case of Neuroblastoma in a Young Mother—A Case Report
Authors: Passudetti Valerio , De Leo Luca , Maselli Filippo , Pellegrino Raffaello , Brindisino Fabrizio
Published: October 2022

Background: Neuroblastoma (NB) is the most common form of paediatric malignancy, responsible for up to 15% of cancer deaths in children, whereas in adults, its onset is a rarer event, despite being characterized by greater lethality. The purpose of this case report was to describe the clinical presentation, physical examination, and clinical decision-making process in a patient with Neuroblastoma mimicking thoracic spine pain of musculoskeletal origin. Methods: a thirty-two-year-old mother complained of thoracic spine pain on her left vertebral side and in her left periscapular muscles; her pain was constant, deep, and worse at night; she also experienced pain during physical exertion of her upper limbs; the patient also reported pain in her left breast. Results: the physiotherapist’s anamnesis and physical examination led him to suspect the need for an extra-expertise pathology and to refer his patient to another medical specialist; the subsequent investigations revealed a poorly differentiated Neuroblastoma ALK + (IIC) in the posterior mediastinum on the left; the patient underwent surgery excision after 4 months. Conclusions: differential screening should be a physiotherapist’s fundamental skill in their patients’ clinical management, especially in direct access cases; the physiotherapist has an ethical and moral duty to conduct differential screening, in order to rule out extra-expertise pathologies—both when patients self-refer for rehabilitation assessment, and when they are referred by other practitioners.
COVID-19 and Lockdown, as Lived and Felt by University Students
Authors: Branquinho Cátia , Guedes Fábio Botelho , Cerqueira Ana , Marques-Pinto Alexandra , Branco Amélia , Galvão Cecília , Sousa Joana , Goulão Luís F. , Bronze Maria Rosário , Viegas Wanda , Matos Margarida Gaspar de
Published: October 2022

In the last 2 years, the COVID-19 pandemic has spread all over the world, forcing the closure of universities, among other unusual measures in recent history. (1) Background: This work is based on the study HOUSE-ULisbon, a survey carried out during the second confinement (March–May 2021) in Portugal with the collaboration of all the Faculties of the University of Lisbon (UL). The present work aims to explore gender differences in how first-year college students experienced and felt COVID-19 and the second confinement. (2) Methods: A questionnaire was carried out. In total, 976 university students (19.66 years (SD = 4.033); Min = 17 and Max = 65) from the first year of the UL were included, of which 69.5% (n = 678) were female, and 30.5% were male (n = 298). SPSS v. 26 was used for quantitative data and MAXQDA 2020 for qualitative data. (3) Results: Overall, students reported various symptoms of physical and mental discomfort (especially females). Statistically significant differences were found in the problems that could arise from the pandemic, such as the prevalence of higher anxiety and worries by females, and online gaming by males. In coping strategies, differences were found in leisure and family relationships, with greater difficulty on the female side. Social interaction was perceived as difficult or very difficult by both genders. As strategies for future pandemics, they highlighted a concerted effort between the government and media in the transmission of messages to the population, facilitating information, knowledge and adoption of protective behaviors. (4) Conclusions: These results are important data for activating or maintaining resources and services for first-year university students, who in some university institutions were supported during the pandemic by psychological, material (e.g., computers, internet), and financial support measures, which are now diminished or extinct. The impacts on their lives will certainly not be extinguished post-pandemic, and health, education, and public policy measures should be prioritized for this group. These results are important data for activating resources and services for students, informing health and education professionals, and supporting public policies.
Exploring Public Discussions Regarding COVID-19 Vaccinations on Microblogs in China: Findings from Machine Learning Algorithms
Authors: Dang Qiong , Li Shixian
Published: October 2022

Large-scale, widespread COVID-19 vaccination is the most effective means of cutting off the spread of the novel coronavirus and establishing an immune barrier. Due to the large population base in China, it has been a very difficult task to establish such an immune barrier. Therefore, this study aims to explore the public’s discussions related to COVID-19 vaccinations on microblogs and to detect their sentiments toward COVID-19 vaccination so as to improve the vaccination rate in China. This study employed machine learning methods in the field of artificial intelligence to analyze mass data obtained from SinaWeibo. A total of 1,478,875 valid microblog texts were collected between December 2020 and June 2022, the results of which indicated that: (1) overall, negative texts (38.7%) slightly outweighed positive texts (36.1%); “Good” (63%) dominated positive texts, while “disgust” (44.6%) and “fear” (35.8%) dominated negative texts; (2) six overarching themes related to COVID-19 vaccination were identified: public trust in the Chinese government, changes in daily work and study, vaccine economy, international COVID-19 vaccination, the COVID-19 vaccine’s R&D, and COVID-19 vaccination for special groups. These themes and sentiments can clarify the public’s reactions to COVID-19 vaccination and help Chinese officials’ response to vaccine hesitancy. Furthermore, this study seeks to make up for the lack of focus on big data in public health and epidemiology research, and to provide novel insights for future studies.
Influence of Loads and Loading Position on the Muscle Activity of the Trunk and Lower Extremity during Squat Exercise
Authors: Park Ju-Hyung , Lee Seung-Jea , Shin Ho-Jin , Cho Hwi-Young
Published: October 2022

This study aimed to investigate the effect of the load and bar position on trunk and lower extremity muscle activity during squat exercise. High bar back squats (HBBS) and low bar back squats (LBBS) were performed in random order at 50%, 60%, and 70% loads of one repetition maximum by 28 experienced healthy adult men who had been performing squats for at least one year. Before the experiment, the maximal voluntary contraction of the vastus medialis, vastus lateralis, rectus femoris, biceps femoris, rectus abdominis, transverse abdominis, external oblique, and erector spinae muscles was measured by means of surface electromyography. In addition, eccentric and concentric exercises were performed for 3 s each to measure the muscle activity. There was a significant difference in muscle activity according to the load for all muscles in the eccentric and concentric phases (p < 0.05), indicating that muscle activity increased as the load increased. In addition, in the comparison between HBBS and LBBS, significant differences were shown in all lower extremity muscles and all trunk muscles except for the external oblique in the concentric phase according to the bar position (p < 0.05). HBBS showed a higher muscle activity of the lower extremity in the eccentric and concentric phases than in LBBS, while LBBS showed a higher muscle activity of the trunk muscle in the eccentric and concentric phases than in HBBS (p < 0.05). HBBS requires more force in the lower extremity than LBBS and is particularly advantageous in strengthening the muscular strength of the quadriceps. In contrast, LBBS requires more muscle activity in the trunk than HBBS and is more effective in carrying heavier loads because of the advantage of body stability. This study suggests that rehabilitation experts apply the bar position and load as important variables affecting the intensity and method of training for target muscle strengthening of the lower extremities and trunk.
Did Human Microbes Affect Tourist Arrivals before the COVID-19 Shock? Pre-Effect Forecasting Model for Slovenia
Authors: Gričar Sergej , Bojnec Štefan
Published: October 2022

In 2020, with a substantial decline in tourist arrivals slightly before the time of COVID-19, the innovative econometric approach predicted possible responses between the spread of human microbes (bacteria/viruses) and tourist arrivals. The article developed a conceptually tested econometric model for predicting an exogenous shock on tourist arrivals driven by the spread of disease using a time series approach. The reworked study is based on an autoregressive integrated moving average (ARIMA) model to avoid spurious results. The periods of robust empirical study were obtained from the data vectors i) from January 2008 to December 2018 and ii) from January 2008 to December 2020. The data were obtained from the National Institute of Public Health (NIPH) and the Statistical Office of the Republic of Slovenia. The ARIMA model predicted the number of declines in tourist arrivals for the approaching periods due to the spread of viruses. Before the outbreak of COVID-19, pre-pandemic results confirmed a one-fifth drop in tourist arrivals in the medium term. In the short term, the decline could be more than three-quarters. A further shock can be caused by forecasted bacterial infections; less likely to reduce tourist demand in the long term. The results can improve the evidence for public health demand in risk reduction for tourists as possible patients. The data from the NIPH are crucial for monitoring public health and tourism management as a base for predictions of unknown events.