bluuebunny/crossref_metadata_2025 huggingface dataset:
Poly-phase alteration history of the kaolinitized ‘Cava di Caolino’ volcanics (Lipari Island, southern Italy)
Authors: Decrée S. , Bernard A. , Yans J. , De Putter Th.
Published: June 2005

A 65 m thick altered volcanic profile was studied in the Cava di Caolino (Lipari Island) in order to (1) identify the alteration event(s), (2) model these events, and (3) propose estimates of the degree of chemical alteration (CIA, chemical index of alteration). Two mineral parageneses were identified: one comprising silico-aluminous phases, with well crystallized kaolinite (type 1), and the other comprising sulphates, with kaolinite as fracture infilling (type 2). The geochemistry of fluids analysed from a local hot spring (40°C, pH ≈ 8) allowed modelling of the observed silico-aluminous paragenesis. A later fumarolic event is suggested to be responsible for the sulphate paragenesis. The main stage of hydrothermal alteration lasted for ~50 ky, was marked by an increase in the CIA from the protolith (~52) to the alterites (~98), and resulted in the alteration of a 65 m thick series. Such data could be used to predict the alteration of volcanic rocks around underground nuclear waste repositories.
How reliable is the K-Ar glauconite chronometer? A case study of Eocene sediments from the Isle of Wight
Authors: Clauer N. , Huggett J. M. , Hillier S.
Published: June 2005

K-Ar ages of Eocene glauconite pellets from the Isle of Wight are related to quantified amounts of older glauconite pellets and to occurrences of detrital mica/illite particles that might have been added to synsedimentary pellets during reworking processes. Addition of older glauconite did not significantly bias the K-Ar dates, as the results most often provide the expected stratigraphic reference ages or even significantly lower ages. Alternatively, K-Ar dates significantly greater than those suggested by the stratigraphy appear to result from non-glauconized detrital mica in the pellets which is not always removable, even by high-gradient magnetic separation.

Unexpected ‘old’ K-Ar glauconite ages do not result from misapplication of the method, but from inability to systematically identify and remove the `contaminant' particles from glauconite splits. Analysis of highly evolved glauconite separates is definitely appropriate for reliable isotopic age determinations, but it might not be enough for the final selection, as long-lasting diagenesis might have taken over the synsedimentary process. Much if not all depends on the separation and characterization of the separates, but also on the completion of the glauconitization process which effects cannot always be anticipated.
Synthesis and characterization of montmorillonite-type phyllosilicates in a fluoride medium
Authors: Reinholdt M. , Miehé-Brendlé J. , Delmotte L. , Le Dred R. , Tuilier M.-H.
Published: June 2005

The fluorine route is thoroughly investigated for the hydrothermal synthesis of montmorillonite in the Na2O-MgO-Al2O3-SiO2-H2O system. Using the optimal conditions suggested by Reinholdt et al. (2001) for the crystallization of pure montmorillonites with the formula Na2x(Al2(1-x)Mg2x☐)Si4O10(OH)2, several parameters (x, Mg content, duration of crystallization, F/Si atomic ratio, pH, nature of counterbalance cation) are varied independently from their ideal values. The products are analysed by various techniques (X-ray diffraction, thermogravimetric analysis-differential thermal analysis, 29Si, 27Al and 19F magic angle spinning-nuclear magnetic resonance). It appears that a pure montmorillonite can only be obtained within a narrow x range (0.10 ≤ x ≤ 0.20). The presence of F in the starting hydrogel and the crystallization time also have significant effects on the purity of the final products. It is shown that a small amount of fluorine is needed for the crystallization of pure montmorillonite phyllosilicates.
Late Mesozoic—Cenozoic clay mineral successions of southern Iran and their palaeoclimatic implications
Authors: Khormali F. , Abtahi A. , Owliaie H. R.
Published: June 2005

Clay minerals of calcareous sedimentary rocks of southern Iran, part of the old Tethys area, were investigated in order to determine their origin and distribution, and to reconstruct the palaeoclimate of the area. Chemical analysis, X-ray diffraction, transmission electron microscopy, scanning electron microscopy, and thin-section studies were performed on the 16 major sedimentary rocks of the Fars and Kuhgiluyeh Boyerahmad Provinces.

Kaolinite, smectite, chlorite, illite, palygorskite and illite-smectite interstratified minerals were detected in the rocks studied. The results revealed that detrital input is possibly the main source of kaolinite, smectite, chlorite and illite, whilein situneoformation during the Tertiary shallow saline and alkaline environment could be the dominant cause of palygorskite occurrences in the sedimentary rocks.

The presence of a large amount of kaolinite in the Lower Cretaceous sediments and the absence or rare occurrence of chlorite, smectite, palygorskite and illite are in accordance with the warm and humid climate of that period. Smaller amounts of kaolinite and the occurrence of smectite in Upper Cretaceous sediments indicate the gradual shift from warm and humid to more seasonal climate. The occurrence of palygorskite and smectite and the disappearance of kaolinite in the late Palaeocene sediments indicate the increase in aridity which has probably continued to the present time.
Combining DLS, XRD, SEM-EDAX and EXAFS in the study of Zn(II) retention on a palygorskitic clay
Authors: Aisa C. , Alvarez-Puebla R. A. , Blasco J. , Echeverría J. C. , Garrido J. J.
Published: June 2005

Clay materials play a key role in determining the retention capacity of a soil, and are widely used in waste treatments. One of the most commonly used clays is palygorskite. The aim of this research is to determine the chemical species formed by Zn when retained in a palygorskitic clay material. Adsorption isotherm analysis is useful in studying the retention process, because it provides a macroscopic view of the retention phenomena. Complementary techniques are needed in order to study the different retention processes. Sorption isotherms of Zn on palygorskitic clay were carried out; the supernatant was analysed by means of dynamic light scattering (DLS) and the residues by using X-ray diffraction (XRD), scanning electron microscopy-energy dispersive angle X-ray (SEM-EDAX)analysis and extended X-ray absorption fine structure (EXAFS). Isotherm analysis shows that the global retention process could be due to the sum of two separate processes, adsorption and surface precipitation via solid-solution. This is supported by DLS, which shows that ζ potential increases as the Zn(II) is retained onto clay surfaces but remains constant during the precipitation process. The XRD pattern corresponding to the Zn-clay system showed weak new peaks, probably from zincite. The microanalysis by X-ray fluorescence of several spots selected for their different electronic densities indicated that the retained Zn was randomly distributed across the clay surface. Analysis by EXAFS supports the hypothesis of retention via adsorption and solid-solution surface precipitation.
Mineralogy, chemical characteristics and stabilities of Cetylpyridinium-exchanged smectite
Authors: Koh S. M. , Song M. S. , Takagi T.
Published: June 2005

CP (Cetylpyridinium)-exchanged smectite was manufactured by the cationic exchange reaction of Na-smectite with CP. The adsorption behaviour shows a typical L-type isotherm representing continuous and stable adsorption in excess of twice the cation exchange capacity of CP. CP-smectite shows a strong interlayer (d001) expansion to 40.2 Å when an amount of CP equivalent to 140% of the CEC is exchanged into smectite. CP-smectite is characterized by strong alkalinity (pH 9.3), low swelling (4.5 ml/2 g), low viscosity (3.9 mPa s), and strong and fast flocculation. These characteristics are in striking contrast to Na-smectite except for the alkalinity. CP-smectite shows no signs of desorption of the organic species under varying pH conditions (pH 2–12). Desorption is slight even when washed three times with DH2O (distilled water). Pyrolysis of CP-smectite begins at 250°C and ends at 400°C. These stable and persistent characteristics and the relatively low price of CP point to the likely increased use of CP-smectite as an organoclay.
The adsorption of berberine by montmorillonite and thermo-XRD analysis of the organo-clay complex
Authors: Cohen E. , Joseph T. , Lapides I. , Yariv S.
Published: June 2005

The adsorption of the large cationic alkaloid berberine by montmorillonite in aqueous suspensions was investigated. An adsorption isotherm of berberine and desorption isotherms of exchangeable Na+, K+, Mg2+ and Ca2+ were determined by chemical analysis of the non-adsorbed berberine and the released cations in the supernatants. The isotherms suggest that the adsorption of berberine takes place in two different types of reaction mechanism: (1) cation exchange, and (2) a secondary organophyllic adsorption. The cation exchange occurring first is followed by the secondary adsorption which is accompanied by Cl- adsorption. Monovalent cations are exchanged before the divalent cations.

Samples loaded with increasing amounts of berberine were examined by X-ray diffraction at room temperature, and after gradual heating up to 420°C. The basal spacing of the loaded samples after the thermal treatment became 1.28 nm, indicating the formation of carbon in the interlayer, thus proving that the adsorbed berberine was located in the interlayer space. At room temperature the basal spacing of samples loaded with 8 and 15 mmol of berberine per 100 g of clay was 1.28–1.30 nm indicating that in the interlayer space berberine cations were lying parallel to the alumino-silicate layers. The basal spacing of a sample with 36 mmol of berberine per 100 g of clay was 1.48 nm. This spacing does not give conclusive information on the arrangement of berberine in the interlayer. The spacing of samples with 57–147 mmol of berberine per 100 g of clay was 1.59 nm, suggesting the presence of bilayers of berberine in the interlayers. During thermal dehydration, the spacing of samples with 36–73 mmol of berberine per 100 g of clay decreased, but that of samples with 105–147 mmol of berberine per 100 g of clay increased to 1.66 nm. These samples contain organophilic adsorbed berberine and it is suggested that due to the thermal treatment berberine was tilted relative to the TOT lamellae, with anhydrous Cl- between the berberine cations.
Adsorption of cetyltrimethylammonium ions on an acid-activated smectite and their thermal stability
Authors: Kooli F. , Magusin P. C. M. M.
Published: June 2005

The intercalation of the cationic surfactant cetyltrimethylammonium (C16TMA) into the interlayer of an acid-activated clay in the presence of different anions has been studied in detail. When Br or OH anions were used, the basal spacing increased significantly, the increase being related to the loading concentration of the surfactant solution. For intercalated compounds prepared from the hydroxide form, the basal spacing at room temperature varied from 1.6 to 3.8 nm. However, for organoclays prepared from the surfactant bromide, the basal spacing is almost loading- independent (1.9 nm). The use of hydroxide and bromide at higher pH is crucial to intercalating larger amounts of C16TMA cations and, hence, to improving the exfoliation of the silicate sheets. Magic-angle spinning 13C nuclear magnetic resonance spectroscopy indicates that the intercalated surfactants exhibit a significant degree of gauche conformation. According to in situ powder X-ray diffraction, an increase of the basal spacing to 4.08 nm is observed at intermediate temperatures of 50 to 150°C for organoclay with an initial basal spacing of 3.7 nm. At higher temperatures, decomposition of the surfactant occurs and the basal spacing decreases to ~1.4 nm.
A km-scale illite alteration zone in sedimentary wall rocks adjacent to a hydrothermal fluorite vein deposit
Authors: Brockamp O. , Clauer N.
Published: June 2005

In a study of wall-rock alteration in the 1.4 km long Würmtal adit at the Käfersteige hydrothermal vein deposit (northern Black Forest, Germany) illite was found to be the only clay mineral within the Bunter sandstone and intercalated claystone. Illite occurs mainly as a detrital mineral in the claystone, whereas it is hydrothermally neoformed in the sandstone, either in the pores or as an alteration product of K-feldspar. The extensive occurrence of authigenic illite along the entire 1.4 km long profile confirms that the fluids migrated far into the sandstone.

The authigenic illite formed during a first pulse of high-temperature fluids (Th of ~220°C) with a low salinity (~1 wt.% NaCleq). These fluids also dissolved Sr and Rb from detrital illite of the claystones at the vein. A later hydrothermal pulse with a lower temperature (~70–150°C) and higher salinity (21–28 wt.% NaCleq) silicified the sandstone adjacent to the vein and caused partial substitution of OH- by F- in the structure of the detrital and neoformed illite along the profile.

Within analytical error, the K-Ar dates for the neoformed illite of the <2 µm fraction are the same along the profile (~150 Ma). During this hydrothermal process, the age of the detrital illite within the claystone was reset from 310 to 190 Ma. The illite-rich <0.2 µm fractions yield ages of ~142 Ma (sandstone) indicating a Jurassic origin. The uniform age data for illite in the sandstone and in the claystone are probably due to extensive migration of hot fluids through the wall rocks.

The hydrothermal fluids are attributed to recycled meteoric water and brines that ascended from the basement into the cover rocks during the opening of the North Atlantic and/or the nearby Tethys area.
Polymorphisms in signal transducer and activator of transcription 3 and lung function in asthma
Authors: Litonjua Augusto A , Tantisira Kelan G , Lake Stephen , Lazarus Ross , Richter Brent G , Gabriel Stacey , Silverman Eric S , Weiss Scott T
Published: June 2005

Background

Identifying genetic determinants for lung function is important in providing insight into the pathophysiology of asthma. Signal transducer and activator of transcription 3 is a transcription factor latent in the cytoplasm; the gene (STAT3) is activated by a wide range of cytokines, and may play a role in lung development and asthma pathogenesis.

Methods

We genotyped six single nucleotide polymorphisms (SNPs) in the STAT3 gene in a cohort of 401 Caucasian adult asthmatics. The associations between each SNP and forced expiratory volume in 1 second (FEV1), as a percent of predicted, at the baseline exam were tested using multiple linear regression models. Longitudinal analyses involving repeated measures of FEV1 were conducted with mixed linear models. Haplotype analyses were conducted using imputed haplotypes. We completed a second association study by genotyping the same six polymorphisms in a cohort of 652 Caucasian children with asthma.

Results

We found that three polymorphisms were significantly associated with baseline FEV1: homozygotes for the minor alleles of each polymorphism had lower FEV1 than homozygotes for the major alleles. Moreover, these associations persisted when we performed an analysis on repeated measures of FEV1 over 8 weeks. A haplotypic analysis based on the six polymorphisms indicated that two haplotypes were associated with baseline FEV1. Among the childhood asthmatics, one polymorphism was associated with both baseline FEV1 and the repeated measures of FEV1 over 4 years.

Conclusion

Our results indicate that genetic variants in STAT3, independent of asthma treatment, are determinants of FEV1 in both adults and children with asthma, and suggest that STAT3 may participate in inflammatory pathways that have an impact on level of lung function.
Preponderance of the oncogenic V599E and V599K mutations in B-raf kinase domain is enhanced in melanoma cutaneous/subcutaneous metastases
Authors: Deichmann Martin , Thome Marianne , Benner Axel , Kirschner Martin , Hassanzadeh Judith , Kurzen Hjalmar
Published: June 2005

Background

Downstream of Ras, the serine/threonine kinase B-raf has been reported to be mutated, among other carcinomas, in a substantial subset of primary melanomas with a preponderance of mutations within the kinase domain including the activating V599E and V599K transitions.

Methods

We here investigated a representative series of 60 resection specimens of cutaneous and subcutaneous melanoma metastases for the presence of mutations within the activation segment (exon 15) of the B-raf kinase domain by polymerase chain reaction (PCR) and single-strand conformation polymorphism (SSCP) gel electrophoresis.

Results

Sequencing of cloned PCR-SSCP amplicons resulted in 24 (40%) samples harbouring somatic mutations which is not exceeding the mutation frequency in recently investigated primary melanomas. The activating mutation T1796A was present in 24/60 (40%) resection specimens, followed in frequency by the oncogenic g1795A mutation in 8/60 (13%) cases. As to the B-raf protein sequence, the acidic amino acid transitions V599E and V599K were predicted in 19/60 (32%) and 6/60 (10%) cases, resepectively, but were not associated with enhanced risk for subsequent metastasis in patients' follow up. In comparison to the primary melanomas that we recently investigated, the spectrum of predicted B-raf protein mutations narrowed significantly in the cutaneous/subcutaneous metastases. Unexpectedly, V599 and V599E mutations were absent in cutaneous/subcutaneous metastases derived from acrolentiginous melanomas as preceding primary tumours.

Conclusion

During transition from primary melanomas towards cutaneous/subcutaneous metastases, the spectrum of predicted B-raf mutations narrows significantly. Focusing on the V599E and V599K, these oncogenic mutations are likely to affect melanocyte-specific pathways controlling proliferation and differentiation.
Measles vaccine coverage and factors related to uncompleted vaccination among 18-month-old and 36-month-old children in Kyoto, Japan
Authors: Matsumura Takayo , Nakayama Takeo , Okamoto Shigeru , Ito Hideko
Published: June 2005

Background

Due to low vaccine coverage, Japan has not only experienced outbreaks of measles but has also been exporting it overseas. This study aims to survey measles vaccine coverage and the factors uncompleted vaccination among community-living children.

Methods

Subjects were the parents whose children had undergone either an 18-month or a 36-month checkup publicly provided by Kyoto City during November 2001 to January 2002. An anonymous self-administered questionnaire survey was conducted.

Results

The coverage was 73.2% among the 18-month-old children (n = 2707) and 88.9% among the 36-month-old children (n = 2340), respectively. The following characteristics of mothers were related to uncompleted measles vaccination: aged below 30, working, concerned about the adverse events of the vaccine, and had insufficient knowledge. Similarly, the following characteristics among children were related to uncompleted measles vaccination: not the first-born child, interacting with other children in group settings. The coverage was the lowest among the children whose mothers were concerned about the adverse events of the vaccine without proper knowledge of measles and its vaccination.

Conclusion

To increase vaccine coverage among children, parents' awareness about measles and vaccination against it should be promoted, especially for working mothers. Efforts to enhance access to vaccination services and to communicate with parents about changing vaccination schedules are necessary.
Simultaneous measurements of cytoplasmic Ca2+ responses and intracellular pH in neutrophils of localized aggressive periodontitis (LAP) patients
Authors: Herrmann Jens Martin , Kantarci Alpdogan , Long Heidi , Bernardo John , Hasturk Hatice , Wray Lewis V , Simons Elizabeth R , Van Dyke Thomas E
Published: June 2005

In view of the reports that polymorphonuclear leukocytes (PMN) of patients with localized aggressive periodontitis (LAP) exhibit hyper-responsiveness to stimulation, it has been suggested that such abnormalities could lead to PMN-mediated tissue damage during inflammation. To determine whether these abnormalities include signal transduction, we compared cytoplasmic calcium concentration (Δ[Ca2+]i) and cytoplasmic pH (ΔpHi) changes, early stimulus responses to chemotactic agents, of LAP versus control (C)-PMN and explored whether these could be modulated by sensitizing cytokines or calcium channel-blocking agents. PMN responses of LAP patients were compared with age- and gender-matched controls. Δ[Ca2+]i and ΔpHi were measured fluorimetrically using 1H-indole-6-carboxylic acid, 2-[4-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-3-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-1 and 2′,7′-bis-(carboxyethyl)-5(6)-carboxyfluorescein as respective probes. Not only was the maximal calcium response to chemoattractants higher in LAP-PMN, but also their subsequent intracellular calcium redistribution was significantly slower. The slower calcium redistribution of LAP-PMN, but not their higher maximal calcium response, was successfully mimicked in C-PMN treated with Nifedipine™ or 1-[b-[3-(4-methoxyphenyl)propoxy]-4-methoxyphenethyl]-1H-imidazole-HCl, both known to be inhibitors of membrane-associated calcium influx, but this redistribution was not affected when inhibitors of other calcium influx mechanisms, Diltiazem™ or Verapamil™, were used. Taken together, our findings indicate that certain early stimulus responses are aberrant in LAP-PMN, that internal redistribution of cytoplasmic-free calcium is compromised, and, additionally, that a membrane-associated Ca2+ transport defect may be present.
Professional phagocytic granulocytes of the bony fish gilthead seabream display functional adaptation to testicular microenvironment
Authors: Chaves-Pozo Elena , Mulero Victoriano , Meseguer José , Ayala Alfonsa García
Published: June 2005

It has been shown previously that professional phagocytic granulocytes are present in the testis of the gilthead seabream, a seasonal breeding teleost that offers an excellent model for studying the testicular regression process that occurs in seasonal testicular involution and sex change. It is unexpected that testicular granulocytes produce interleukin-1β, a regulator for spermatogonia proliferation in mammals, but are not involved in the elimination of degenerative germ cells. Here, we show that phagocytosis and reactive oxygen intermediate (ROI) production were suppressed dramatically in testicular phagocytic granulocytes, compared with their level of activity in the head-kidney, the main hematopoietic organ in fish. Furthermore, testicular-conditioned media modulated migration, phagocytosis, and ROI production of head-kidney phagocytic granulocytes, and the addition of testicular cells impaired their ROI production capacity. Until now, monocytes/macrophages were believed to be the only innate immune cells able to develop into functional subsets, whereas neutrophils only infiltrate the tissues upon infection or inflammation. Our findings demonstrate, however, that fish professional phagocytic granulocytes also display functional adaptation to different microenvironments and strongly suggest a role for these cells in the reorganization of the testis during post-spawning.
Receptors and lytic mediators regulating anti-tumor activity by the leukemic killer T cell line TALL-104
Authors: Brando Clara , Mukhopadhyay Sunil , Kovacs Eniko , Medina Rosa , Patel Pritesh , Catina Tracey L , Campbell Kerry S , Santoli Daniela
Published: June 2005

The major histocompatibility complex nonrestricted cytotoxic leukemic T cell line T acute lymphoblastic leukemia (TALL)-104 is being pursued as a therapeutic agent for cancer. However, the receptors and effector mechanisms responsible for its broad tumoricidal function remain undefined. Here, we examined the roles played by natural cytotoxicity receptors (NCR), killer cell immunoglobulin-like receptors, cytolytic granule components, and tumor necrosis factor (TNF) family members in tumor recognition and lysis by TALL-104 cells. The perforin-granzyme pathway, TNF-related apoptosis-inducing ligand (TRAIL), and Fas were each involved in the lysis of particular tumor targets by TALL-104. Furthermore, phorbol 12-myristate 13-acetate/ionomycin treatment induced surface expression of Fas-L and TRAIL. In addition, supernatants from CD3-stimulated TALL-104 cultures exhibited antiproliferative activity, which was blocked 50–90% by anti-TNF-α monoclonal antibody (mAb). Although negative for the NCR natural killer (NK)p44, this cell line was found to express NKp46. An anti-NKp46 antibody strongly blocked TALL-104-mediated lysis of certain targets and directly induced cytokine production, granule release, and redirected lysis responses. Anti-NKG2D and anti-2B4 also stimulated redirected cytotoxicity by TALL-104. By contrast, anti-NKG2A mAb did not stain the cells or inhibit killing responses. Alternatively, KIR3DL2 was detected on TALL-104, and expression of its reported ligand, human leukocyte antigen (HLA)-A, on target cells provided protection from cytotoxicity. Thus, NKp46, NKG2D, and 2B4 are activating receptors, and KIR3DL2 is an inhibitory receptor on TALL-104. The data demonstrate the ability of TALL-104 cells to recognize a wide variety of tumors with NK cell receptors and kill them with a broad arsenal of cytolytic effector mechanisms, including cytolytic granules and TNF family ligands.
Fat bodies: developing geographical research agendas
Authors: Longhurst Robyn
Published: June 2005

Over the past decade geographers, especially social, cultural, critical and feminist geographers, have shown a keen interest in the mutually constitutive relationship between bodies and spaces. There is, however, one aspect of embodiment that has escaped geographers' attention, that is, fatness. In this paper I aim to develop some geographical research agendas that examine fatness as an important component of subjectivity. The paper begins by discussing some of the discourses surrounding fat and fatness, such as fat oppression, fat-phobia and fat acceptance. Secondly, there is an examination of some possible reasons why fat bodies have been for the most part absent from geographical research. Thirdly, the paper draws together the limited references that geographers have made to fat bodies, spaces and places. Finally, the paper offers suggestions for developing geographical research agendas that address fatness using a range of scales and theoretical perspectives.
Ecological and geographical scale: parallels and potential for integration
Authors: Sayre Nathan F.
Published: June 2005

Scale has emerged as a major issue in both ecology and geography in recent decades. Little effort has been made to compare these parallel debates, however, or to seek an integrated conception of scale across the two disciplines. This paper argues that such an integration is possible, even between ecology and human geography—the subfield of geography seemingly most removed from ecological concerns and methods. In both disciplines, globalization has lent practical urgency to problems of scale, revealing deeper theoretical issues. Geographers have helped impel ecologists to take space and scale seriously, and the epistemological insight that scale is produced (rather than given a priori) should be applied to ecological as well as social phenomena. Ecologists' conceptual distinctions and methodological guidelines regarding scale, meanwhile, can help resolve `the scale question' in critical human geography. Scale is both a methodological issue inherent to observation (its epistemological moment) and an objective characteristic of complex interactions within and among social and natural processes (its ontological moment). These processes and interactions—rather than scale per se—should be the object of research, with particular attention to nonlinearities or thresholds of change.
Gender equality and progress of gender studies in Japanese geography: a critical overview
Authors: Murata Yohei
Published: June 2005

This paper examines gender equality in Japanese academic geography and provides an overview of gender studies undertaken in the discipline. First, I point out the serious gender imbalance which exists in Japanese geography, through an examination of data on gender participation in academic societies, publications and faculty promotions. Secondly, I provide an overview of geographical research on gender in Japan. Thirdly, I point out as major issues concerning gender studies in Japanese geography that the Japanese male geographer tends to misunderstand gender and ignore sexuality. In conclusion, I suggest that Japanese male geographers need to realize that gender is their own issue and that there is a need to conduct men's studies in Japanese geography.
Global hunting grounds: power, scale and ecology in the negotiation of conservation
Authors: MacDonald Kenneth Iain
Published: June 2005

Increasingly, large international conservation organizations have come to rely upon market-oriented interventions, such as sport trophy hunting, to achieve multiple goals of biodiversity protection and ‘development’. Such initiatives apply an understanding of ‘nature’-defined through an emerging discourse of global ecology-to incorporate local ecologies within the material organizational sphere of capital and transnational institutions, generating new forms of governmentality at scales inaccessible to traditional means of discipline such as legislation and enforcement. In this paper, I historicize debates over ‘nature’ in a region of northern Pakistan, and demonstrate how local ecologies are becoming subject to transnational institutional agents through strategies similar to those used by colonial administrators to gain ecological control over their ‘dominions’. This contemporary reworking of a colonialist ethic of conservation relies rhetorically on a discourse of global ecology, and on ideological representations of a resident population as incapable environmental managers, to assert and implement an allegedly scientifically and ethically superior force better able to respond to assumed degradation. In undertaking such disciplinary projects, international conservation organizations rely on, and produce, a representation of ecological space as ‘global’ to facilitate the attainment of translocal political-ecological goals.
Wild horses in a ‘European wilderness’: imagining sustainable development in the post-Communist countryside
Authors: Schwartz Katrina Z. S.
Published: June 2005

When the Soviet Union and its satellite regimes collapsed, they bequeathed to successor states an unexpected dual legacy: an outsized, backward agrarian sector on the one hand and a wealth of undeveloped nature, rich in biological diversity, on the other. Popular perceptions of the region centre on nightmarish images of environmental devastation, but environmentalists on both sides of the former ‘Iron Curtain’ are increasingly recognizing the unintended benefits to nature of Communist underdevelopment. Eight of the post-Communist states have now consummated their long-awaited ‘return to Europe’, but as they begin a new era as European Union members, they confront a critical developmental challenge. Faced with declining agricultural prospects and growing Western interest in Eastern nature, what to do with a large and underemployed rural population and an ever-expanding area of marginal farmland? This article contributes to a growing literature on the political ecology of post-Communist transformation in the ‘Second World’ through a case study from Latvia. At Lake Pape, the Latvian program office of WWF International has implemented a Western-funded project in ecosystem restoration and eco-tourism promotion involving introduction of wild horses in a remote but ecologically rich coastal wetland area. I explore diverse Latvian responses to the polysemic Western narratives of sustainable rural development and biodiversity conservation that have been borne into Eastern Europe along with Western aid monies. Local mediation of these narratives is shaped by the struggle between competing ‘agrarian’ and ‘internationalist’ understandings of national geography, identity and developmental destiny that have structured cultural and political discourse since the emergence of Latvian nationalism 150 years ago.
Producing social nature in the Mexican countryside
Authors: Klooster Daniel J.
Published: June 2005

In most countries in Latin America, rural areas remain as populated as, or more populated than, in 1950. People continue to live in rural areas despite the declining economic viability of agriculture and the availability of work elsewhere. Through an application of the production of nature argument, enriched by explicit attention to the production of culture and the agency of nature, I attempt to resolve that apparent paradox. In a case study illustrating the argument, agriculture has declined in importance over several decades, while craft production and temporary, cyclical emigration has increased. Remaining agricultural activities and craft production utilize natural stocks and processes through the application of family labour, with minimal recourse to a money economy. Cyclical emigration and remittances from relatives also support the economic maintenance of rural lives. Together, these activities permit the social reproduction of households that send members to find work elsewhere. At the scale of North America, therefore, Mexican nature subsidizes the cheap reproduction of labourers working in cities and commercial agriculture in both Mexico and the United States. At the scale of the village, nature enables people to cobble together livelihoods that support households and villages. But more fundamentally, people produce culture through everyday activities of production and consumption; and so nature provides the necessary context for the productive activities that define and give meaning to what households and village communities are, and what it means to be an individual member.
War landscapes as ‘battlefields’ of collective memories: reading the Reflections at Bukit Chandu, Singapore
Authors: Muzaini Hamzah , Yeoh Brenda S. A.
Published: June 2005

This paper examines the commemoration of the Second World War in the non-Western context of Singapore. It argues that the Singaporean state has viewed the war-fought when Singapore was still part of a larger colonial entity that was British Malaya-as a means of raising the awareness of a ‘shared history’ among its citizens. We first outline how the task of appropriating Singaporean war memory in the postcolonial present may be potentially inflected by a myriad of local as well as transnational challenges. Then, drawing on one particular national memoryscape dedicated to the war, the Reflections at Bukit Chandu, we explore some of the strategies the state has adopted to mitigate these. Finally, we illustrate, from visitors’ perspectives, how contestations over the site’s (post)colonial geography, history and representations of race have continued to make the site highly contentious. On a larger canvas, we demonstrate how national appropriations of the past can become fraught ‘battlefields’ of collective memories from ‘within’ as well as ‘without’ the nation.
The aerodynamics of hovering flight inDrosophila
Authors: Fry Steven N. , Sayaman Rosalyn , Dickinson Michael H.
Published: June 2005

Using 3D infrared high-speed video, we captured the continuous wing and body kinematics of free-flying fruit flies, Drosophila melanogaster,during hovering and slow forward flight. We then `replayed' the wing kinematics on a dynamically scaled robotic model to measure the aerodynamic forces produced by the wings. Hovering animals generate a U-shaped wing trajectory, in which large drag forces during a downward plunge at the start of each stroke create peak vertical forces. Quasi-steady mechanisms could account for nearly all of the mean measured force required to hover, although temporal discrepancies between instantaneous measured forces and model predictions indicate that unsteady mechanisms also play a significant role. We analyzed the requirements for hovering from an analysis of the time history of forces and moments in all six degrees of freedom. The wing kinematics necessary to generate sufficient lift are highly constrained by the requirement to balance thrust and pitch torque over the stroke cycle. We also compare the wing motion and aerodynamic forces of free and tethered flies. Tethering causes a strong distortion of the stroke pattern that results in a reduction of translational forces and a prominent nose-down pitch moment. The stereotyped distortion under tethered conditions is most likely due to a disruption of sensory feedback. Finally, we calculated flight power based directly on the measurements of wing motion and aerodynamic forces, which yielded a higher estimate of muscle power during free hovering flight than prior estimates based on time-averaged parameters. This discrepancy is mostly due to a two- to threefold underestimate of the mean profile drag coefficient in prior studies. We also compared our values with the predictions of the same time-averaged models using more accurate kinematic and aerodynamic input parameters based on our high-speed videography measurements. In this case, the time-averaged models tended to overestimate flight costs.
Acoustic characteristics of underwater tail slaps used by Norwegian and Icelandic killer whales (Orcinus orca) to debilitate herring(Clupea harengus)
Authors: Simon Malene , Wahlberg Magnus , Ugarte Fernando , Miller Lee A.
Published: June 2005

Norwegian killer whales debilitate prey by slapping their tails into herring schools. These underwater tail slaps produce a thud-like sound. It is unclear whether this sound is caused by cavitation and/or physical contact between herring and whale tail. Also the forces causing debilitation of the fish are not understood. Here we present an acoustic analysis of underwater tail slaps using a multi-channel wide (150 kHz) band recording system. Underwater tail slaps produced by Norwegian killer whales generated sounds consisting of multiple pulses with source levels of 186±5.4 dB (pp)re.1 μPa at 1 m (±1 s.d., N=4). The –3 dB and 97% energy bandwidths were 36.8±22.5 kHz and 130.5±17.5 kHz(±1 s.d., N=13), respectively, with a centre frequency of 46.1±22.3 kHz. The similarities between the acoustic properties of underwater tail slaps recorded from killer whales in Norway, and thud-like sounds recorded from killer whales in Iceland suggest that Norwegian and Icelandic killer whales use similar hunting techniques. The acoustic characteristics of sounds produced by underwater tail slaps were similar to the ones from other cavitation sound sources described in the literature, both in term of temporal and frequency features as well as in source level. We suggest that multiple factors generated by the tail slaps like particle fluctuations, turbulence, pressure changes and physical impact cause debilitation of herring.
Cuvier's beaked whale (Ziphius cavirostris) head tissues:physical properties and CT imaging
Authors: Soldevilla Melissa S. , McKenna Megan F. , Wiggins Sean M. , Shadwick Robert E. , Cranford Ted W. , Hildebrand John A.
Published: June 2005

Tissue physical properties from a Cuvier's beaked whale (Ziphius cavirostris) neonate head are reported and compared with computed tomography (CT) X-ray imaging. Physical properties measured include longitudinal sound velocity, density, elastic modulus and hysteresis. Tissues were classified by type as follows: mandibular acoustic fat, mandibular blubber, forehead acoustic fat (melon), forehead blubber, muscle and connective tissue. Results show that each class of tissues has unique,co-varying physical properties. The mandibular acoustic fats had minimal values for sound speed (1350±10.6 m s–1) and mass density (890±23 kg m–3). These values increased through mandibular blubber (1376±13 m s–1,919±13 kg m–3), melon (1382±23 m s–1, 937±17 kg m–3), forehead blubber(1401±7.8 m s–1, 935±25 kg m–3) and muscle (1517±46.8 m s–1,993±58 kg m–3). Connective tissue had the greatest mean sound speed and density (1628±48.7 m s–1,1087±41 kg m–3). The melon formed a low-density,low-sound-speed core, supporting its function as a sound focusing organ. Hounsfield unit (HU) values from CT X-ray imaging are correlated with density and sound speed values, allowing HU values to be used to predict these physical properties. Blubber and connective tissues have a higher elastic modulus than acoustic fats and melon, suggesting more collagen structure in blubber and connective tissues. Blubber tissue elastic modulus is nonlinear with varying stress, becoming more incompressible as stress is increased. These data provide important physical properties required to construct models of the sound generation and reception mechanisms in Ziphius cavirostris heads, as well as models of their interaction with anthropogenic sound.
The anhydrobiotic potential and molecular phylogenetics of species and strains ofPanagrolaimus(Nematoda, Panagrolaimidae)
Authors: Shannon Adam J. , Browne John A. , Boyd Jacqueline , Fitzpatrick David A. , Burnell Ann M.
Published: June 2005

Members of the genus Panagrolaimus are bacterial-feeding nematodes that occupy a diversity of niches ranging from Antarctic and temperate soils to terrestrial mosses. Some members of this genus are able to survive extreme desiccation by entering into a state of suspended animation known as anhydrobiosis. We have assembled a collection of Panagrolaimusspecies and strains and have investigated their anhydrobiotic phenotypes. Our data show that within the genus Panagrolaimus there is a continuum of strains ranging from those unable to survive exposure to low relative humidity(RH) without prior preconditioning at high RH (slow desiccation strategists),through strains that have limited ability to survive rapid desiccation but whose anhydrobiotic ability improves upon preconditioning, to strains such as P. superbus that can readily survive immediate exposure to severe desiccation (fast desiccation strategists). Using this panel of nematodes we investigated the effect of preincubation at high RH on the accumulation of trehalose and on the nematodes' anhydrobiotic potential. We found that there is a strong correlation between trehalose induction and anhydrobiotic survival in Panagrolaimus. Furthermore, the high trehalose levels observed in fully hydrated P. superbus (10% dry mass) suggest that constitutive expression of trehalose pre-adapts this fast dehydration strategist to combat desiccation. All the strains observed, regardless of survival rates, undertook both coiling and clumping, which has the effect of reducing surface area and slowing the rate of water loss during desiccation. Phylogenetic analyses were carried out to investigate whether the observed anhydrobiotic phenotypes were the result of convergent evolution or represented a single phylogenetic lineage. These analyses, derived from alignments of the rDNA ITS and D3 sequences, indicate that the strongly anhydrobiotic strains of Panagrolaimus form a single phylogenetic lineage, which is separate from the weakly anhydrobiotic strains. The weakly anhydrobiotic strains are also phylogenetically divergent from each other. Our data indicate that Panagrolaimus has the potential to be an excellent model system for the investigation of molecular aspects of nematode anhydrobiosis.
Temperature-dependent protein synthesis capacities in Antarctic and temperate (North Sea) fish (Zoarcidae)
Authors: Storch Daniela , Lannig Gisela , Pörtner Hans O.
Published: June 2005

For an evaluation of effects of seasonal cold acclimation and evolutionary cold adaptation on protein synthesis capacity, the protein synthesis apparatus was isolated from the gills and white muscle of Antarctic eelpout Pachycara brachycephalum and North Sea eelpout Zoarces viviparus. Both species had been acclimated to 0°C (control) and 5°C (Antarctic) and 5°C and 10°C (North Sea control). The translational capacities of the protein synthesis machineries were determined in an optimised cell-free in vitro system. The results demonstrate that tissues from the polar zoarcid possess cold-adapted protein synthesis machineries, indicated by low activation energies and, especially, high RNA translational capacities at similar RNA:protein ratios when compared to temperate zoarcids at 10°C. When both species were brought to 5°C, the temperate species displayed cold compensated protein synthesis capacities caused by elevated RNA:protein ratios. Warm exposure (from 0 to 5°C) of the Antarctic zoarcid revealed a capacity for thermal acclimation indicated by a reduction in protein synthesis capacities associated with lower RNA:protein ratios.
Context-dependent changes in strength and efficacy of leg coordination mechanisms
Authors: Dürr Volker
Published: June 2005

Appropriate coordination of stepping in adjacent legs is crucial for stable walking. Several leg coordination rules have been derived from behavioural experiments on walking insects, some of which also apply to arthropods with more than six legs and to four-legged walking vertebrates. Three of these rules affect the timing of stance–swing transition [rules 1 to 3(sensu Cruse)]. They can give rise to normal leg coordination and adaptive responses to disturbances, as shown by kinematic simulations and dynamic hardware tests. In spite of their importance to the study of animal walking, the coupling strength associated with these rules has never been measured experimentally. Generally coupling strength of the underlying mechanisms has been considered constant rather than context-dependent.

The present study analyses stepping patterns of the stick insect Carausius morosus during straight and curve walking sequences. To infer strength and efficacy of coupling between pairs of sender and receiver legs, the likelihood of the receiver leg being in swing is determined, given a certain delay relative to the time of a swing–stance (or stance–swing) transition in the sender leg. This is compared to a corresponding measure for independent, hence uncoupled, step sequences. The difference is defined as coupling strength. The ratio of coupling strength and its theoretical maximum is defined as efficacy.

Irrespective of the coordination rule, coupling strength between ipsilateral leg pairs is at least twice that of contralateral leg pairs, being strongest between ipsilateral hind and middle legs and weakest between contralateral middle legs. Efficacy is highest for inhibitory rule 1, reaching 84–95% for ipsilateral and 29–65% for contralateral leg pairs. Efficacy of excitatory rules 2 and 3 ranges between 35–56% for ipsilateral and 8–21% for contralateral leg pairs. The behavioural transition from straight to curve walking is associated with context-dependent changes in coupling strength, increasing in both outer leg pairs and decreasing between inner hind and middle leg. Thus, the coordination rules that are thought to underlie many adaptive properties of the walking system,themselves adapt in a context-dependent manner.