bluuebunny/crossref_metadata_2025 huggingface dataset:
Quality of Life in Yoga Practitioners—Research Conducted on a Population of Polish Yogis
Authors: Piekorz Zuzanna , Radzimińska Agnieszka , Lewandowski Andrzej , Ossowski Roman
Published: September 2022

Background: The aim of the study is to determine the quality of life of a population of healthy adult yoga practitioners and the correlation between the features of yoga practice and the quality of life. Methods: A total of 300 people aged 35–50 years were examined and divided into two groups. The respondent (SG; n = 150) was a yoga practitioner, and the control (CG; n = 150) did not practice yoga. The original questionnaire and the quality-of-life questionnaire (WHOQOL-BREF) were used. The chi-square test, the Kolmogorov–Smirnov test, the Mann–Whitney U test, the ANOVA Kruskal–Wallis test and the linear regression model were used. The significance level was set at p < 0.05. Results: Yoga practitioners are characterised by a significantly greater overall satisfaction with the quality of life (U = 9794.50; p < 0.05), a higher level of health satisfaction (U = 9194.50; p < 0.01) and higher satisfaction with quality life in terms of the results of somatic domains (U = 7579.00; p < 0.001), psychological (U = 8554.00; p < 0.001) and environmental domains (U = 7919.5; p < 0.001). A relationship was observed between the experience of yoga practice and the assessment of the quality of life in terms of physical functioning. Conclusions: The practice of adult yoga positively influences the assessment of their quality of life in the physical, mental and environmental spheres.
Disease-Specific Knowledge, Physical Activity, and Physical Functioning Examination among Patients with Chronic Non-Specific Low Back Pain
Authors: Hock Márta , Járomi Melinda , Prémusz Viktória , Szekeres Zsolt János , Ács Pongrác , Szilágyi Brigitta , Wang Zhe , Makai Alexandra
Published: September 2022

Physical activity, physical functioning, and pain are some of the most critical factors of low back pain (LBP) treatment and prevention, but it was unknown that the back school program (BSP) influences the physical activity level of the patients with LBP. Data from 306 healthy patients and patients with chronic non-specific low back pain (cnsLBP) were used. We used the Global Physical Activity Questionnaire (GPAQ), the Low Back Pain Knowledge Questionnaire (LKQ), the visual analog scale, and the Roland–Morris Disability Questionnaire (RMDQ). The significance level was set at p < 0.05. The amount of sedentary time in cnsLBP patients enrolled in the BSP was significantly lower compared to the other two groups (p < 0.001). Significantly higher moderate-intensity activities, leisure time activities, and active transportation were observed in the cnsLBP patients enrolled in the BSP than in the other two groups (p < 0.001). RMDQ scores and the pain intensity of the cnsLBP patients enrolled in the BSP were significantly lower than in patients with LBP receiving only exercise therapy (p < 0.001). The physical activity level and low-back-pain-specific knowledge was significantly higher, while back-related disability and pain intensity were significantly lower among patients with low back pain syndrome who participated in a back school program.
Comparative Transcriptome Analysis of Two Peach Rootstocks Uncovers the Effect of Gene Differential Expression on Nitrogen Use Efficiency
Authors: Chen Qiuju , Lian Min , Guo Jian , Zhang Binbin , Yang Sankui , Huang Kexin , Peng Futian , Xiao Yuansong
Published: September 2022

Nitrogen is an important nutrient element that limits plant growth and yield formation, but excessive nitrogen has negative effects on plants and the environment. It is important to reveal the molecular mechanism of high NUE (nitrogen use efficiency) for breeding peach rootstock and variety with high NUE. In this study, two peach rootstocks, Shannong–1 (S) and Maotao (M), with different NUE were used as materials and treated with 0.1 mM KNO3 for transcriptome sequencing together with the control group. From the results of comparison between groups, we found that the two rootstocks had different responses to KNO3, and 2151 (KCL_S vs. KCL_M), 327 (KNO3_S vs. KCL_S), 2200 (KNO3_S vs. KNO3_M) and 146 (KNO3_M vs. KCL_M) differentially expressed genes (DEGs) were identified, respectively, which included multiple transcription factor families. These DEGs were enriched in many biological processes and signal transduction pathways, including nitrogen metabolism and plant hormone signal transduction. The function of PpNRT2.1, which showed up-regulated expression under KNO3 treatment, was verified by heterologous expression in Arabidopsis. The plant height, SPAD (soil and plant analyzer development) of leaf and primary root length of the transgenic plants were increased compared with those of WT, indicating the roles of PpNRT2.1 in nitrogen metabolism. The study uncovered for the first time the different molecular regulatory pathways involved in nitrogen metabolism between two peach rootstocks and provided gene reserve for studying the molecular mechanism of nitrogen metabolism and theoretical basis for screening peach rootstock or variety with high NUE.
Response of Rice with Overlapping Growth Stages to Water Stress by Assimilates Accumulation and Transport and Starch Synthesis of Superior and Inferior Grains
Authors: Wang Xinpeng , Fu Jinxu , Min Zhaosen , Zou Detang , Liu Hualong , Wang Jingguo , Zheng Hongliang , Jia Yan , Yang Luomiao , Xin Wei , Sun Bin , Zhao Hongwei
Published: September 2022

Drought stress at jointing–booting directly affects plant growth and productivity in rice. Limited by natural factors, the jointing and booting stages of short-growth-period rice varieties are highly overlapped in high-latitude areas, which are more sensitive to water deficit. However, little is known about the dry matter translocation in rice and the strategies of starch synthesis and filling of superior and inferior grains under different drought stress was unclear. In this study, the rice plants were subjected to three degrees of drought stress (−10 kPa, −25 kPa, −40 kPa) for 15 days during the jointing–booting stage; we investigated dry matter accumulation and translocation, grain filling and enzyme activities to starch synthesis of superior and inferior grains in rice with overlapping growth stages from 2016 to 2017. The results showed that drought stress significantly reduced dry matter accumulation in the stems and leaves. Mild and moderate drought increased dry matter translocation efficiency. However, severe drought stress largely limited the dry matter accumulation and translocation. A large amount of dry matter remains in vegetative organs under severe drought stress. The high content in NSC in stem and sheath plays a key role in resisting drought stress. The drought stress at jointing–booting directly caused a change in the grain filling strategy. Under moderate and severe drought, the grain-filling active period of the superior grains was shortened to complete the necessary reproductive growth. The grain-filling active period of the inferior grains was significantly prolonged to avoid a decrease in grain yield. The significant decrease in the grain-filling rate of the superior and inferior grains caused a reduction in the thousand-grain weight. In particular, the influence of the grain-filling rate of inferior grains on the thousand-grain weight was more significant. Drought stress changed the starch synthesis strategies of the superior and inferior grains. Soluble starch synthase and starch branching enzyme activities of inferior grains increased significantly under drought stress. GBSS activity was not sensitive to drought stress. Therefore, amylose content was decreased and amylopectin synthesis was enhanced under drought stress, especially in inferior grains.
Ablation of the Presynaptic Protein Mover Impairs Learning Performance and Decreases Anxiety Behavior in Mice
Authors: Schleicher Eva Maria , Bayer Thomas A. , Iseni Trendelina , Ott Frederik Wilhelm , Wagner Jannek Moritz , Viotti Julio S. , Dresbach Thomas , Bouter Yvonne
Published: September 2022

The presynaptic protein Mover/TPRGL/SVAP30 is absent in Drosophila and C. elegans and differentially expressed in synapses in the rodent brain, suggesting that it confers specific functions to subtypes of presynaptic terminals. In order to investigate how the absence of this protein affects behavior and learning, Mover knockout mice (KO) were subjected to a series of established learning tests. To determine possible behavioral and cognitive alterations, male and female 8-week-old KO and C57Bl/6J wildtype (WT) control mice were tested in a battery of memory and anxiety tests. Testing included the cross maze, novel object recognition test (NOR), the Morris water maze (MWM), the elevated plus maze (EPM), and the open field test (OF). Mover KO mice showed impaired recognition memory in the NOR test, and decreased anxiety behavior in the OF and the EPM. Mover KO did not lead to changes in working memory in the cross maze or spatial reference memory in the MWM. However, a detailed analysis of the swimming strategies demonstrated allocentric-specific memory deficits in male KO mice. Our data indicate that Mover appears to control synaptic properties associated with specific forms of memory formation and behavior, suggesting that it has a modulatory role in synaptic transmission.
MPSI Manifestations and Treatment Outcome: Skeletal Focus
Authors: De Ponti Giada , Donsante Samantha , Frigeni Marta , Pievani Alice , Corsi Alessandro , Bernardo Maria Ester , Riminucci Mara , Serafini Marta
Published: September 2022

Mucopolysaccharidosis type I (MPSI) (OMIM #252800) is an autosomal recessive disorder caused by pathogenic variants in the IDUA gene encoding for the lysosomal alpha-L-iduronidase enzyme. The deficiency of this enzyme causes systemic accumulation of glycosaminoglycans (GAGs). Although disease manifestations are typically not apparent at birth, they can present early in life, are progressive, and include a wide spectrum of phenotypic findings. Among these, the storage of GAGs within the lysosomes disrupts cell function and metabolism in the cartilage, thus impairing normal bone development and ossification. Skeletal manifestations of MPSI are often refractory to treatment and severely affect patients’ quality of life. This review discusses the pathological and molecular processes leading to impaired endochondral ossification in MPSI patients and the limitations of current therapeutic approaches. Understanding the underlying mechanisms responsible for the skeletal phenotype in MPSI patients is crucial, as it could lead to the development of new therapeutic strategies targeting the skeletal abnormalities of MPSI in the early stages of the disease.
Studies on the Phytochemical Profile of Ocimum basilicum var. minimum (L.) Alef. Essential Oil, Its Larvicidal Activity and In Silico Interaction with Acetylcholinesterase against Aedes aegypti (Diptera: Culicidae)
Authors: Botelho Anderson de Santana , Ferreira Oberdan Oliveira , de Oliveira Mozaniel Santana , Cruz Jorddy Neves , Chaves Sandro Henrique dos Reis , do Prado Alejandro Ferraz , Nascimento Lidiane Diniz do , da Silva Geilson Alcantara , Amarante Cristine Bastos do , Andrade Eloisa Helena de Aguiar
Published: September 2022

Aedes aegypti L. (Diptera: Culicidae) is an important transmitter of diseases in tropical countries and controlling the larvae of this mosquito helps to reduce cases of diseases such as dengue, zika and chikungunya. Thus, the present study aimed to evaluate the larvicidal potential of the essential oil (EO) of Ocimum basilicum var. minimum (L.) Alef. The EO was extracted by stem distillation and the chemical composition was characterized by gas chromatography coupled with mass spectrometry (GC/MS and GC-FID). The larvicidal activity of EO was evaluated against third instar Ae. aegypti following World Health Organization (WHO) standard protocol and the interaction of the major compounds with the acetylcholinesterase (AChE) was evaluated by molecular docking. The predominant class was oxygenated monoterpenes with a concentration of 81.69% and the major compounds were limonene (9.5%), 1,8-cineole (14.23%), linalool (24.51%) and methyl chavicol (37.41%). The O. basilicum var. minimum EO showed unprecedented activity against third instar Ae. aegypti larvae at a dose-dependent relationship with LC50 of 69.91 (µg/mL) and LC90 of 200.62 (µg/mL), and the major compounds were able to interact with AChE in the Molecular Docking assay, indicating an ecological alternative for mosquito larvae control.
C3H Expression Is Crucial for Methyl Jasmonate Induction of Chicoric Acid Production by Echinacea purpurea (L.) Moench Cell Suspension Cultures
Authors: Ravazzolo Laura , Ruperti Benedetto , Frigo Marco , Bertaiola Oriana , Pressi Giovanna , Malagoli Mario , Quaggiotti Silvia
Published: September 2022

Echinacea purpurea (L.) Moench is one of the most economically important medicinal plants, cultivated worldwide for its high medicinal value and with several industrial applications in both pharmaceutical and food industries. Thanks to its various phytochemical contents, including caffeic acid derivatives (CADs), E. purpurea extracts have antioxidant, anti-inflammatory, and immuno-stimulating properties. Among CADs, chicoric acid is one of the most important compounds which have shown important pharmacological properties. The present research was aimed at optimizing the production of chicoric acid in E. purpurea cell culture. Methyl jasmonate (MeJa) at different concentrations and for different duration of treatments was utilized as elicitor, and the content of total polyphenols and chicoric acid was measured. Several genes involved in the chicoric acid biosynthetic pathway were selected, and their expression evaluated at different time points of cell culture growth. This was performed with the aim of identifying the most suitable putative molecular markers to be used as a proxy for the early prediction of chicoric acid contents, without the need of expensive quantification methods. A correlation between the production of chicoric acid in response to MeJa and an increased response to oxidative stress was also proposed.
Multi-Objective Optimization of the Process Parameters in Electric Discharge Machining of 316L Porous Stainless Steel Using Metaheuristic Techniques
Authors: Singh Himanshu , Patrange Praful , Saxena Prateek , Puri Yogesh M.
Published: September 2022

Electric discharge machining is an essential modern manufacturing process employed to machine porous sintered metals. The sintered 316L porous stainless steel (PSS) components are widely used in diverse engineering domains, as interconnected pores are present. The PSS material has excellent lightweight and damping properties and superior mechanical and metallurgical properties. However, conventional machining techniques are not suitable for porous metals machining. Such techniques tend to block the micro-pores, resulting in a decrease in porous materials’ breathability. Thus, the EDM process is an effective technique for porous metal machining. The input process parameters selected in this study are peak current (Ip), pulse on time (Ton), voltage (V), flushing pressure (fp), and porosity. The response parameters selected are material removal rate (MRR) and tool wear rate (TWR). The present work aims to obtain optimum machining process parameters in the EDM of porous sintered SS316L using two meta-heuristic optimization techniques, i.e., Teaching Learning-Based Optimization (TLBO) and Particle Swarm Optimization (PSO) algorithms, to maximize the MRR and minimize the TWR values. In the case of PSS having a 12.60% porosity value, PSO and TLBO algorithms give same optimum machining parameters. However, for PSS having an 18.85% porosity value, the PSO algorithm improves by about 5.25% in MRR and by 5.63% in TWR over the TLBO. In the case of PSS having a 31.11% porosity value, the PSO algorithm improves about 3.73% in MRR and 6.46% in TWR over the TLBO. The PSO algorithm is found to be consistent and to converge more quickly, taking minimal computational time and effort compared to the TLBO algorithm. The present study’s findings contribute valuable information in regulating the EDM performance in machining porous SS316L.
3D Printed Cellulose-Based Filaments—Processing and Mechanical Properties
Authors: Utz Julia , Zubizarreta Jokin , Geis Nico , Immonen Kirsi , Kangas Heli , Ruckdäschel Holger
Published: September 2022

Cellulose is an abundant and sustainable material that is receiving more and more attention in different industries. In the context of additive manufacturing, it would be even more valuable. However, there are some challenges to overcome in processing cellulose-based materials. Therefore, this study used a new thermoplastic cellulose-based granulate to show its potential in filament extrusion and the fused filament fabrication printing process. Furthermore, the mechanical properties were investigated. It was shown that filaments with a suitable and uniform diameter could be produced. A parameter study for printing revealed that adhesion of the material on the bed and between layers was an issue but could be overcome with a suitable set of parameters. Tensile bars with different orientations of 0°, +/−45°, and 90° were printed and compared with injection-molded samples. It could be shown that different mechanisms (single strand breakage, shear failure) caused fracture for different printing orientations. In comparison with injection-molding, the printed parts showed lower mechanical properties (moduli of 74–95%, a tensile strength of 47–69%, and an elongation at break of 29–60%), but an improvement could be seen compared with earlier reported direct granule printing. The study showed that FFF is a suitable process for the new cellulose-based material to fabricate samples with good mechanical properties.
Flexible Magnetic Metasurface with Defect Cavity for Wireless Power Transfer System
Authors: Hiep Le Thi Hong , Khuyen Bui Xuan , Tung Bui Son , Ngo Quang Minh , Lam Vu Dinh , Pham Thanh Son
Published: September 2022

In this paper, we present a flexible magnetic metamaterial structure for enhancing the efficiency of wireless power transfer (WPT) systems operating at 13.56 MHz. The metasurface between transmitter (Tx) and receiver (Rx) coils of the WPT system is constructed of a 3 × 5 metamaterial unit cell array with a total size of 150 × 300 mm2. Most metamaterial structures integrated into WPT systems are in planar configurations with a rigid substrate, which limits practical applications. The proposed metasurface is fabricated on an FR-4 substrate with a thin thickness of 0.2 mm; therefore, it can be bent with radii greater than 80 mm. A defect cavity is formed in the non-homogeneous metasurface by controlling the resonant frequency of the unit cell with an external capacitor. Simulation and measurement results show that the efficiency of the WPT system is significantly enhanced with metasurfaces. The performance of the WPT system can also be optimized with suitable bend profiles of metasurfaces. This proposed flexible metasurface could be widely applied to WPT systems, especially asymmetric, bendable, or wearable WPT systems.
The Effect of Selected Factors on the Strength of Stitches of Upholstery Faux Leather
Authors: Vilhanová Anna , Langová Nadežda , Kłos Robert , Máchová Eliška
Published: September 2022

With their properties, upholstery covering materials significantly influence the quality of upholstered products. These materials form the surface layer of upholstered furniture; any damage to this material is immediately visible to the user. We consider the stitches connecting the covering fabrics to be one of the critical points of the upholstered surface, therefore they must have the required strength. Faux leather is one of the most used upholstery materials. The main aim of the paper is to determine the effect of upholstery faux leather, stitch length, point needle, and needle size on the strength of the stitches and the seam efficiency. The results of the experiment proved the suitability of using a sewing needle LR for joining covering materials such as faux leathers. The highest force to seam a rupture in the joints was achieved in the direction of the warp yarns of the underlying layer of PU (Polyurethane) faux leather with a stitch length of 4 mm and needle type LR/90. The highest seam efficiency was achieved with seams in the direction of the weft yarns of the PVC (Polyvinyl chloride) faux leather underlying layer with a stitch length of 4 mm and needle type LR/90. If the underlying layer of faux leather is a fabric with canvas binding, a higher seam efficiency of joints is assumed.
Electric Arc Furnace Dust Recycled in 7075 Aluminum Alloy Composites Fabricated by Spark Plasma Sintering (SPS)
Authors: Soares Elder , Bouchonneau Nadège , Alves Elizeth , Alves Kleber , Filho Oscar Araújo , Mesguich David , Chevallier Geoffroy , Khalile Nouhaila , Laurent Christophe , Estournès Claude
Published: September 2022

The reuse of industrial waste, such as electric arc furnace dust (EAFD) as reinforcement in aluminum matrix composites (AMC), is still little explored even though it has shown potential to improve the mechanical properties, such as hardness and mechanical strength, of AMCs. To propose a new alternative for EAFD recycling, AA7075-EAFD composites were produced by spark plasma sintering (SPS). The starting powders were prepared by high-energy milling with different weight fractions of EAFD in two particle size ranges added to an AA7075 matrix. SEM shows that the distribution of reinforcement particles in the matrix is homogeneous with no agglomeration of the particles. XRD patterns of initial powders and the SPS-sintered (SPSed) samples suggest that there was no reaction during sintering (no additional peaks were detected). The relative density of all SPSed samples exceeded 96.5%. The Vickers microhardness of the composites tended to increase with increasing EAFD content, increasing from 108 HV (AA7075 without reinforcement) up to 168 HV (56% increase). The maximum microhardness value was obtained when using 15 wt.% EAFD with a particle size smaller than 53 μm (called G1), showing that EAFD presents a promising potential to be applied as reinforcement in AA7075 matrix composites.
Investigation of the Tribological Behavior and Microstructure of Plasma-Cladded Fe–Cr–Mo–Ni–B Coating
Authors: Chen Junfu , Zhang Fenglong , Ren Xianghui , Wu Yaoshen , Han Shanguo , Cai Manxia , Li Zhenglong , Li Likun
Published: September 2022

In this study, an Fe–Cr–Mo–Ni–B coating was prepared using plasma cladding on Cr5 steel substrate. The microstructure, phase evolution and tribological performance of the Fe–Cr–Mo–Ni–B coating were investigated. The microstructure is mainly composed of Mo2FeB2, Fe2B, α-Fe, γ-Fe and MoB. The process of phase evolution in the coating was observed in situ by HT-CLSM. The Mo2FeB2 phase with good thermodynamic stability can exist in the high-temperature liquid phase. It also has a phenomenon of connection and merging and turns into different morphology during the plasma cladding process. The hardness value of coating was much higher than the base metal, and the hardness value of Mo2FeB2 (785.5 HV) was higher than the eutectic matrix (693.2 HV). The wear mechanisms of the cladding under dry sliding were primarily caused by adhesive wear, accompanying slight oxidation wear. The Mo2FeB2 phase has an important effect on the wear resistance property.
Quantitative Assessment of Liver Impairment in Chronic Viral Hepatitis with [99mTc]Tc-Mebrofenin: A Noninvasive Attempt to Stage Viral Hepatitis-Associated Liver Fibrosis
Authors: Jocius Donatas , Vajauskas Donatas , Mikelis Kipras , Jokubauskiene Skirmante , Jakutiene Jolita , Strupas Kestutis , Tamosiunas Algirdas E.
Published: September 2022

Background and objectives—Chronic viral hepatitis B and C infections are one of the leading causes of chronic liver impairment, resulting in liver fibrosis and liver cirrhosis. An early diagnosis with accurate liver fibrosis staging leads to a proper diagnosis, thus tailoring correct treatment. Both invasive and noninvasive techniques are used in the diagnosis and staging of chronic liver impairment. Those techniques include liver biopsy, multiple serological markers (as either single tests or combined panels), and imaging examinations, such as ultrasound or magnetic resonance elastography. Nuclear medicine probes may also be employed in staging liver fibrosis, although the literature scarcely reports this. The purpose of our study was to investigate whether a dynamic liver scintigraphy with [99mTc]Tc-mebrofenin has any value in staging or grading chronic liver damage. Materials and Methods—We prospectively enrolled patients with chronic viral hepatitis B and C infection referred for liver biopsy. All patient underwent dynamic liver scintigraphy with 99mTc-mebrofenin prior to liver biopsy. Dynamic liver scintigraphy was performed immediately after intravenous tracer injection for 30 min scanning time. Multiple scintigraphy parameters were calculated (whole liver lobe and focal area time to peak (TTP), 30 min to peak ratio (30/peak), whole lobe and focal area slope index in 350 s (slope_350). Liver biopsy took place shortly after imaging. Results—We found that many dynamic scintigraphic parameters are positively or negatively associated with different stages of liver fibrosis. The main parameters that showed most value are the ratio between 30 min and the peak of the dynamic curve (30/peak_dex (ratio)), and liver clearance corrected for body surface area and liver area (LCL_m2_dm2 (%/min/m2/dm2)). Conclusions—Our present study proves that conducting dynamic liver scintigraphies with [99mTc]Tc-mebrofenin has potential value in staging liver fibrosis. The benefits of this method, including whole liver imaging and direct imaging of the liver function, provide an advantage over presently used quantitative imaging modalities.
Experimental Investigations on the Structure of Yeast Mitochondrial Pyruvate Carriers
Authors: Li Ling , Wen Maorong , Run Changqing , Wu Bin , OuYang Bo
Published: September 2022

Mitochondrial pyruvate carrier (MPC) transports pyruvate from the cytoplasm into the mitochondrial matrix to participate in the tricarboxylic acid (TCA) cycle, which further generates the energy for the physiological activities of cells. Two interacting subunits, MPC1 and MPC2 or MPC3, form a heterodimer to conduct transport function. However, the structural basis of how the MPC complex transports pyruvate is still lacking. Here, we described the detailed expression and purification procedures to obtain large amounts of yeast MPC1 and MPC2 for structural characterization. The purified yeast MPC1 and MPC2 were reconstituted in dodecylphosphocholine (DPC) micelles and examined using nuclear magnetic resonance (NMR) spectroscopy, showing that both subunits contain three α-helical transmembrane regions with substantial differences from what was predicted by AlphaFold2. Furthermore, the new protocol producing the recombinant MPC2 using modified maltose-binding protein (MBP) with cyanogen bromide (CNBr) cleavage introduced general way to obtain small membrane proteins. These findings provide a preliminary understanding for the structure of the MPC complex and useful guidance for further studies.
Microbial Responses to Various Types of Chemical Regents during On-Line Cleaning of UF Membranes
Authors: Gao Zeyuan , Chen Qiuying , Song Xiaolan , Wang Jingwei , Cai Weiwei
Published: September 2022

Ultrafiltration is widely used to treat various environmental waters, and on-line membrane cleaning with various chemical reagents is frequently employed to sustain the filtration flux. However, the residue of cleaning agents in the ultrafiltration system is unavoidable, which may affect microbiological properties and biofilm formation during the next-round filtration. By investigating the changes in microbial characteristics, and their biofouling behaviors after exposure to HCl, NaOH, NaClO, citric acid (CA), and sodium dodecyl sulfonate (SDS), this study fills a knowledge gap in microbial responses to various types of chemical cleaning agents in an ultrafiltration system. The result shows that HCl, NaOH, and NaClO affect the bacterial properties and subsequent attachment on the membrane surface, while CA and SDS have no obvious influence on microorganisms. Specifically, HCl, NaOH, and NaClO reduce the hydrophobicity and mean size of suspended microorganisms, increase the extracellular polymeric substances (EPS) release, and trigger intracellular reactive oxygen species (ROS) generation, resulting in the death of a large quantity of microorganisms. Due to the self-protecting strategy, plenty of living cells aggregate on the membrane surface and form a cake layer with a stratified structure, causing more severe membrane biofouling.
Analytical Validation of an Assay for Concurrent Measurement of Amino Acids in Dog Serum and Comparison of Amino Acid Concentrations between Whole Blood, Plasma, and Serum from Dogs
Authors: Blake Amanda B. , Ishii Patricia E. , Phillips Robert K. , Lidbury Jonathan A. , Steiner Joerg M. , Suchodolski Jan S.
Published: September 2022

Amino acids play an important role in metabolism. Comprehensive analytical validation of an assay for the concurrent measurement of a large number of amino acids in dogs is lacking, which precludes its usefulness in a clinical setting. Amino acids are often measured in plasma or whole blood. However, serum is commonly used for gastrointestinal diagnostic testing in dogs and is therefore convenient to use. This study aimed to analytically validate an assay for the concurrent measurement of amino acids in dog serum and to evaluate differences in amino acid concentrations in whole blood, plasma, and serum in dogs. Analytical validation of the assay (Biochrom 30+ Amino Acid Analyzer) was performed on fresh or banked serum samples from dogs. Whole blood, plasma, and serum from 36 healthy dogs were analyzed, and concentrations of the three sample types were compared. The assay was demonstrated to be precise, reproducible, accurate, linear, and stable for the measurement of the majority of compounds detected in dog serum. Cystine, glutamic acid, and ethanolamine were shown to be unstable at conditions commonly encountered in clinical settings. Significant differences in concentrations were identified between whole blood, plasma, and serum for 33 of 42 compounds. Amino acid profiles in serum and plasma were more similar to each other than to those in whole blood. While some amino acids are present in similar concentrations in whole blood, plasma, and serum, others are highly dependent on the type of biofluid, and measurements warrant strict adherence to sample type-based reference intervals.
Integrated Analysis of Gut Microbiome and Lipid Metabolism in Mice Infected with Carbapenem-Resistant Enterobacteriaceae
Authors: Zhang Ning , Peng Yuanyuan , Zhao Linjing , He Peng , Zhu Jiamin , Liu Yumin , Liu Xijian , Liu Xiaohui , Deng Guoying , Zhang Zhong , Feng Meiqing
Published: September 2022

The disturbance in gut microbiota composition and metabolism has been implicated in the process of pathogenic bacteria infection. However, the characteristics of the microbiota and the metabolic interaction of commensals–host during pathogen invasion remain more than vague. In this study, the potential associations of gut microbes with disturbed lipid metabolism in mice upon carbapenem-resistant Escherichia coli (CRE) infection were explored by the biochemical and multi-omics approaches including metagenomics, metabolomics and lipidomics, and then the key metabolites–reaction–enzyme–gene interaction network was constructed. Results showed that intestinal Erysipelotrichaceae family was strongly associated with the hepatic total cholesterol and HDL-cholesterol, as well as a few sera and fecal metabolites involved in lipid metabolism such as 24, 25-dihydrolanosterol. A high-coverage lipidomic analysis further demonstrated that a total of 529 lipid molecules was significantly enriched and 520 were depleted in the liver of mice infected with CRE. Among them, 35 lipid species showed high correlations (|r| > 0.8 and p < 0.05) with the Erysipelotrichaceae family, including phosphatidylglycerol (42:2), phosphatidylglycerol (42:3), phosphatidylglycerol (38:5), phosphatidylcholine (42:4), ceramide (d17:1/16:0), ceramide (d18:1/16:0) and diacylglycerol (20:2), with correlation coefficients higher than 0.9. In conclusion, the systematic multi-omics study improved the understanding of the complicated connection between the microbiota and the host during pathogen invasion, which thereby is expected to lead to the future discovery and establishment of novel control strategies for CRE infection.
Microstructural Characteristics of Graphite Microcrystals in Graphitized Coal: Insights from Petrology, Mineralogy and Spectroscopy
Authors: Li Jiuqing , Qin Yong , Chen Yilin , Shen Jian
Published: September 2022

Graphite microcrystals are the product of coal graphitization and widely exist in the graphitized coal of Yongan Coalfield, Fujian Province, China, which is direct mineralogical evidence for the transformation of coal to graphite. Optical microscopy, scanning electron microscopy (SEM) and micro-Raman spectroscopy were used to detect the morphology and microstructure of the in situ graphite microcrystals. The results show that the volume proportion of graphite microcrystals in graphitized coal samples is between 2.39% and 7.32%, and the optical anisotropy of graphite microcrystals is stronger than that of coal macerals. Graphite microcrystals show the occurrence of attached microcrack inner walls or infilling the cell cavity, with several forms of flakes, needles or aggregates. Under optical microscopy of polarized light and with a retarder plate of 1λ, graphite microcrystals show the color of primary yellow and secondary blue, and the two kinds of colors appear alternately when the microscope is rotating. Additionally, flake-like graphite microcrystals with an isochromatic zone diameter of 10−50 μm are the most widely distributed in graphitized coal. Under SEM, graphite microcrystals show a rough and irregular edge and are characterized by flow or bubble film structures along with several pores, which is the product of cooling crystallization after the softening and melting of carbon-containing substances. Moreover, flake-like graphite microcrystals developed interlayer pores with a clear outline of loose stacking and were almost entirely composed of pure carbon; a small amount of oxygen is related to oxygen-containing functional groups or structural defects. The micro-Raman spectra of graphite microcrystals in the first-order region are characterized by low-intensity D1 and D2 bands and a high-intensity G band, and the parameters R1 and R2 vary from 0.21–0.39 and 0.60–0.74, respectively. The second-order micro-Raman spectra of graphite microcrystals are characterized by a higher intensity of the 2D1 band and a lower intensity of the other three bands. The parameter R3, derived from the area ratio of the 2D1 band to all the bands in the second-order region, was proposed. The value of R3 ranges between 0.78 and 0.86, and both of them indicate a higher percentage of graphene plane with a highly internal crystallographic structure. Similar to the parameters R1 and R2 in the first-order micro-Raman spectrum, the parameter R3 is an effective parameter to characterize the ordering degree of the microstructure, which may be used to evaluate the graphitization degree of graphitization coal.
Effect of Ammonium Sulfide on Sulfidization Flotation of Malachite
Authors: Ibrahim Ayman M. , Jia Xiaodong , Su Chao , Cai Jinpeng , Shen Peilun , Liu Dianwen
Published: September 2022

Recently, several studies have shown the positive effect of sulfidization flotation on malachite surfaces and its enhancing methods. Therefore, this paper was focused on the effect of ammonium sulfide and sodium sulfide on the sulfidization of malachite, respectively; this was investigated using different devices such as the micro-flotation tests, Zeta potential measurements, ToF–SIMS, XPS analysis, and FTIR. Thus, Fourier transform infrared spectroscopy results demonstrated that a new characteristic peak of Cu-S bonds was formed and adsorbed on malachite surfaces at 1694 cm−1, as confirmed by XPS analysis. Notably, malachite with ammonium sulfide ions had a significantly higher flotation recovery than malachite with an excess of sodium sulfide ions, as concerns of sulfidization types. Conclusively, all the experiments in this study confirmed that additional copper sulfide products were formed on the malachite surface, increasing the hydrophobicity of the malachite.
From cis-Lobeline to trans-Lobeline: Study on the Pharmacodynamics and Isomerization Factors
Authors: Xu Huan-Hua , Yang Liang , Tang Ming-Xia , Ye An-Ping , Tu Bo-Dan , Jiang Zhen-Hong , Yi Jian-Feng
Published: September 2022

Lobeline is an alkaloid derived from the leaves of an Indian tobacco plant (Lobelia inflata), which has been prepared by chemical synthesis. It is classified as a partial nicotinic agonist and has a long history of therapeutic usage ranging from emetic and respiratory stimulant to tobacco smoking cessation agent. The presence of both cis and trans isomers in lobeline is well known, and many studies on the relationship between the structure and pharmacological activity of lobeline and its analogs have been reported. However, it is a remarkable fact that no studies have reported the differences in pharmacological activities between the two isomers. In this article, we found that different degrees of isomerization of lobeline injection have significant differences in respiratory excitatory effects in pentobarbital sodium anesthetized rats. Compared with cis-lobeline injections, the respiratory excitatory effect was significantly reduced by 50.2% after administration of injections which contained 36.9% trans-lobeline. The study on the influencing factors of isomerization between two isomers shown that this isomerization was a one-way isomerism and only converted from cis to trans, where temperature was the catalytic factor and pH was the key factor. This study reports a new discovery. Despite the widespread use of ventilators, first-aid medicines such as nikethamide and lobeline has retired to second line, but as a nonselective antagonist with high affinity for a4b2 and a3b2 nicotinic acetylcholine receptors (nAChRs). In recent years, lobeline has shown great promise as a therapeutic drug for mental addiction and nervous system disorders, such as depression, Alzheimer disease and Parkinson disease. Therefore, we suggest that the differences between two isomers should be concerned in subsequent research papers and applications.
Objective Supervised Machine Learning-Based Classification and Inference of Biological Neuronal Networks
Authors: Barros Michael Taynnan , Siljak Harun , Mullen Peter , Papadias Constantinos , Hyttinen Jari , Marchetti Nicola
Published: September 2022

The classification of biological neuron types and networks poses challenges to the full understanding of the human brain’s organisation and functioning. In this paper, we develop a novel objective classification model of biological neuronal morphology and electrical types and their networks, based on the attributes of neuronal communication using supervised machine learning solutions. This presents advantages compared to the existing approaches in neuroinformatics since the data related to mutual information or delay between neurons obtained from spike trains are more abundant than conventional morphological data. We constructed two open-access computational platforms of various neuronal circuits from the Blue Brain Project realistic models, named Neurpy and Neurgen. Then, we investigated how we could perform network tomography with cortical neuronal circuits for the morphological, topological and electrical classification of neurons. We extracted the simulated data of 10,000 network topology combinations with five layers, 25 morphological type (m-type) cells, and 14 electrical type (e-type) cells. We applied the data to several different classifiers (including Support Vector Machine (SVM), Decision Trees, Random Forest, and Artificial Neural Networks). We achieved accuracies of up to 70%, and the inference of biological network structures using network tomography reached up to 65% of accuracy. Objective classification of biological networks can be achieved with cascaded machine learning methods using neuron communication data. SVM methods seem to perform better amongst used techniques. Our research not only contributes to existing classification efforts but sets the road-map for future usage of brain–machine interfaces towards an in vivo objective classification of neurons as a sensing mechanism of the brain’s structure.
Shionone-Targeted Pneumolysin to Ameliorate Acute Lung Injury Induced by Streptococcus pneumoniae In Vivo and In Vitro
Authors: Du Runbao , Wang Tian , Lv Hongfa , Zou Yinuo , Hou Xiaoning , Hou Nana , Zhang Peng , Li Hongen , Chi Gefu
Published: September 2022

Streptococcus pneumoniae (S. pneumoniae), as a Gram-positive bacterium, can cause severe bacterial pneumonia, and result in high morbidity and mortality in infected people. Meanwhile, isolated drug-resistant S. pneumoniae is growing, which raises concerns about strategies for combatting S. pneumoniae infection. To disturb S. pneumoniae pathogenicity and its drug-resistance, developing novel anti-infective strategies or compounds is urgent. In this study, the anti-infective effect of shionone was explored. A minimum inhibitory concentration (MIC) assay and growth curve determination were performed to evaluate the effect of the tetracyclic triterpenoid compound shionone against S. pneumoniae. Hemolysis tests, western blotting, oligomerization inhibition assays, and molecular docking were carried out to explore the anti-infective mechanism of shionone. Moreover, the protective effect of shionone was also confirmed in a mousepneumonia model. The results showed that the excellent hemolytic inhibitory activity of shionone was observed at less than 8 μg/mL. Meanwhile, shionone could disturb the oligomerization of pneumolysin (PLY) but did not interfere with PLY expression at less than 4 μg/mL. Molecular docking suggested that shionone targeted the ASP-59, ILE-60, THR-57, PHE-344, and ASN-346 amino acid sites to reduce S. pneumoniae pathogenicity. Furthermore, shionone alleviated lung histopathologic injury and decreased lung bacterial colonization in vivo. The above results showed that shionone could bind to the PLY active pocket under the concentrations of 8 μg/mL and neutralize PLY hemolysis activity to reduce S. pneumoniae pathogenicity in vitro and in vivo.
Improvement Strategies for Stability and Efficiency of Perovskite Solar Cells
Authors: Liu Hongliang , Xiang Ling , Gao Peng , Wang Dan , Yang Jirui , Chen Xinman , Li Shuti , Shi Yanli , Gao Fangliang , Zhang Yong
Published: September 2022

Recently, perovskites have garnered great attention owing to their outstanding characteristics, such as tunable bandgap, rapid absorption reaction, low cost and solution-based processing, leading to the development of high-quality and low-cost photovoltaic devices. However, the key challenges, such as stability, large-area processing, and toxicity, hinder the commercialization of perovskite solar cells (PSCs). In recent years, several studies have been carried out to overcome these issues and realize the commercialization of PSCs. Herein, the stability and photovoltaic efficiency improvement strategies of perovskite solar cells are briefly summarized from several directions, such as precursor doping, selection of hole/electron transport layer, tandem solar cell structure, and graphene-based PSCs. According to reference and analysis, we present our perspective on the future research directions and challenges of PSCs.
Predictive Value of the Advanced Lipoprotein Profile and Glycated Proteins on Diabetic Retinopathy
Authors: Julve Josep , Rossell Joana , Correig Eudald , Rojo-Lopez Marina Idalia , Amigó Nuria , Hernández Marta , Traveset Alicia , Carbonell Marc , Alonso Nuria , Mauricio Didac , Castelblanco Esmeralda
Published: September 2022

This study aimed to assess whether the advanced characteristics of serum lipoprotein subclasses could better predict the risk of developing diabetic retinopathy (DR) and its severity compared to other established risk factors in subjects with type 1 (T1D) and type 2 (T2D) diabetes. This observational, cross-sectional substudy analyzed DR-related data from 309 T1D and 264 T2D subjects. The advanced lipoprotein and glycoprotein profile was determined by nuclear magnetic resonance (NMR) spectroscopy (Liposcale test). NMR analysis of lipoproteins revealed that T1D subjects with DR showed standard non-HDL particles, despite higher IDL lipid concentrations. Notably, IDL lipids were elevated in T1D subjects with worsened DR. VLDL and LDL were smaller, whereas HDL triglycerides were increased in DR compared with non-DR. On the other hand, the T2D subjects with DR showed altered characteristics in the LDL fraction, mainly revealed by a significant decrease in smaller LDL and a reduction in LDL-C. Moreover, the glycoprotein profile did not reveal significant changes among DR groups, regardless of the type of diabetes. However, lipoprotein characteristics and glycoproteins unveiled by NMR analysis did not improve the predictive value of conventional lipids or other traditional, well-established biomarkers of DR in our cohorts.
Dietary Vitamin B Complex: Orchestration in Human Nutrition throughout Life with Sex Differences
Authors: Ali Mennatallah A. , Hafez Hala A. , Kamel Maher A. , Ghamry Heba I. , Shukry Mustafa , Farag Mohamed A.
Published: September 2022

The importance of B complex vitamins starts early in the human life cycle and continues across its different stages. At the same time, numerous reports have emphasized the critical role of adequate B complex intake. Most studies examined such issues concerning a specific vitamin B or life stage, with the majority reporting the effect of either excess or deficiency. Deep insight into the orchestration of the eight different B vitamins requirements is reviewed across the human life cycle, beginning from fertility and pregnancy and reaching adulthood and senility, emphasizing interactions among them and underlying action mechanisms. The effect of sex is also reviewed for each vitamin at each life stage to highlight the different daily requirements and/or outcomes. Thiamine, riboflavin, niacin, pyridoxine, and folic acid are crucial for maternal and fetal health. During infancy and childhood, B vitamins are integrated with physical and psychological development that have a pivotal impact on one’s overall health in adolescence and adulthood. A higher intake of B vitamins in the elderly is also associated with preventing some aging problems, especially those related to inflammation. All supplementation should be carefully monitored to avoid toxicity and hypervitaminosis. More research should be invested in studying each vitamin individually concerning nutritional disparities in each life stage, with extensive attention paid to cultural differences and lifestyles.