bluuebunny/crossref_metadata_2025 huggingface dataset:
Significance of Pulmonary Endothelial Injury and the Role of Cyclooxygenase-2 and Prostanoid Signaling
Authors: Nickl Rosa , Hauser Sandra , Pietzsch Jens , Richter Torsten
Published: January 2023

The endothelium plays a key role in the dynamic balance of hemodynamic, humoral and inflammatory processes in the human body. Its central importance and the resulting therapeutic concepts are the subject of ongoing research efforts and form the basis for the treatment of numerous diseases. The pulmonary endothelium is an essential component for the gas exchange in humans. Pulmonary endothelial dysfunction has serious consequences for the oxygenation and the gas exchange in humans with the potential of consecutive multiple organ failure. Therefore, in this review, the dysfunction of the pulmonary endothel due to viral, bacterial, and fungal infections, ventilator-related injury, and aspiration is presented in a medical context. Selected aspects of the interaction of endothelial cells with primarily alveolar macrophages are reviewed in more detail. Elucidation of underlying causes and mechanisms of damage and repair may lead to new therapeutic approaches. Specific emphasis is placed on the processes leading to the induction of cyclooxygenase-2 and downstream prostanoid-based signaling pathways associated with this enzyme.
U-Net Model with Transfer Learning Model as a Backbone for Segmentation of Gastrointestinal Tract
Authors: Sharma Neha , Gupta Sheifali , Koundal Deepika , Alyami Sultan , Alshahrani Hani , Asiri Yousef , Shaikh Asadullah
Published: January 2023

The human gastrointestinal (GI) tract is an important part of the body. According to World Health Organization (WHO) research, GI tract infections kill 1.8 million people each year. In the year 2019, almost 5 million individuals were detected with gastrointestinal disease. Radiation therapy has the potential to improve cure rates in GI cancer patients. Radiation oncologists direct X-ray beams at the tumour while avoiding the stomach and intestines. The current objective is to direct the X-ray beam toward the malignancy while avoiding the stomach and intestines in order to improve dose delivery to the tumour. This study offered a technique for segmenting GI tract organs (small bowel, big intestine, and stomach) to assist radio oncologists to treat cancer patients more quickly and accurately. The suggested model is a U-Net model designed from scratch and used for the segmentation of a small size of images to extract the local features more efficiently. Furthermore, in the proposed model, six transfer learning models were employed as the backbone of the U-Net topology. The six transfer learning models used are Inception V3, SeResNet50, VGG19, DenseNet121, InceptionResNetV2, and EfficientNet B0. The suggested model was analysed with model loss, dice coefficient, and IoU. The results specify that the suggested model outperforms all transfer learning models, with performance parameter values as 0.122 model loss, 0.8854 dice coefficient, and 0.8819 IoU.
Dated Phylogeny of Astragalus Section Stereothrix (Fabaceae) and Allied Taxa in the Hypoglottis Clade
Authors: Bagheri Ali , Maassoumi Ali Asghar , Brassac Jonathan , Blattner Frank R.
Published: January 2023

The Astragalus subgenus Hypoglottis Bunge, which consists of several sections, is one of the taxonomically most complicated groups in the genus. The Astragalus section Stereothrix Bunge belongs to this subgenus and is a significant element of the Irano-Turanian floristic region. A molecular phylogenetic analysis of this section and its closely related taxa using nuclear ribosomal DNA internal transcribed spacers (ITS) and external transcribed spacer (ETS) regions as well as plastid matK sequences were conducted. Parsimony analyses and Bayesian phylogenetic inference revealed that the section is not monophyletic in its current form, as some taxa belonging to closely related sections such as Hypoglottidei DC. and the Malacothrix Bunge group within the sect. Stereothrix render it paraphyletic. Moreover, species groups belonging to sect. Stereothrix are placed in different clades within the phylogenetic tree of subgenus Hypoglottis, which indicates polyphyly, i.e., multiple independent origins of taxa placed in the sect. Stereothrix. Molecular dating of the group estimated an age of 3.62 (1.73–5.62) My for this assemblage with the major diversification events happening during the last 2 My. Many species groups separated only within the last 0.5 to 1 My. Based on morphological and molecular data, we discuss the phylogenetic relationships of the groups and synonymy of species. In addition, the included taxa of sect. Hypoglottidei are not monophyletic and include species belonging to sects. Hololeuce, Koelziana, Malacothrix, Onobrychoideae, and Ornithodpodium group within the sect. Stereothrix taxa. We conclude that only an analysis including all groups and nearly all species of the sections within the Hypoglottis clade can finally result in an new evolutionary-based system for these taxa.
Computational Investigation of Chirality-Based Separation of Carbon Nanotubes Using Tripeptide Library
Authors: Singh Shrishti , Divecha Heena R. , Ayoola Abimbola , Xavierselvan Marvin , Devlin Jack , Macwan Isaac
Published: January 2023

Carbon nanotubes (CNT) have fascinating applications in flexible electronics, biosensors, and energy storage devices, and are classified as metallic or semiconducting based on their chirality. Semiconducting CNTs have been teased as a new material for building blocks in electronic devices, owing to their band gap resembling silicon. However, CNTs must be sorted into metallic and semiconducting for such applications. Formerly, gel chromatography, ultracentrifugation, size exclusion chromatography, and phage display libraries were utilized for sorting CNTs. Nevertheless, these techniques are either expensive or have poor efficiency. In this study, we utilize a novel technique of using a library of nine tripeptides with glycine as a central residue to study the effect of flanking residues for large-scale separation of CNTs. Through molecular dynamics, we found that the tripeptide combinations with threonine as one of the flanking residues have a high affinity for metallic CNTs, whereas those with flanking residues having uncharged and negatively charged polar groups show selectivity towards semiconducting CNTs. Furthermore, the role of interfacial water molecules and the ability of the tripeptides to form hydrogen bonds play a crucial role in sorting the CNTs. It is envisaged that CNTs can be sorted based on their chirality-selective interaction affinity to tripeptides.
Multifunctional Modified Tumor Cell Membranes-Coated Adjuvant PTX against Melanoma
Authors: Ji Zhonghua , Lin Bingying , Guan Enshuang , Zhou Mingsen , Wang Hui , Hu Ying
Published: January 2023

Melanoma is the deadliest type of skin cancer. Anti-tumor immunotherapy has made great progress in increasing the overall survival of patients. However, many physiological barriers cause low bioavailability of drugs. Cell membranes are becoming increasingly prevalent for assisting drug delivery because of the significant benefits of avoiding host cell barriers. Herein, B16F10 cell membranes (BFMs) were prepared in this study. BFMs could not only act as antigens but also serve as vesicles for vaccines. To trigger potent immunity, BFMs must be taken up by dendritic cells (DCs) and combined with adjuvants to make BFMs overcome the immune tolerance. To avoid circulating BFMs into tumors and quickly internalized by DCs after subcutaneously injection, the antigen-cell penetrating fusion peptide WT(YGRKKRRQRSRRYVDFFVWL) was used to modify BFMs. Additionally, a low dosage of paclitaxel (PTX) can activate DCs via toll-like receptor-4 (TLR-4). Therefore, we developed PTX-loaded micelles using Pluronic® F127. Then, WT-modified BFMs (WT-BFMs) were coated F127-PTX to yield WT-BFMs/ F127-PTX. Optimized WT-BFMs/F127-PTX promoted the cellular uptake and showed remarkable efficacy in eliciting robust antigen-specific cellular and humoral immune responses.
Age-Related Changes of the Synucleins Profile in the Mouse Retina
Authors: Dias Sarah Batista , de Lemos Luísa , Sousa Luís , Bitoque Diogo B. , Silva Gabriela Araújo , Seabra Miguel C. , Tenreiro Sandra
Published: January 2023

Alpha-synuclein (aSyn) plays a central role in Parkinson’s disease (PD) and has been extensively studied in the brain. This protein is part of the synuclein family, which is also composed of beta-synuclein (bSyn) and gamma-synuclein (gSyn). In addition to its neurotoxic role, synucleins have important functions in the nervous system, modulating synaptic transmission. Synucleins are expressed in the retina, but they have been poorly characterized. However, there is evidence that they are important for visual function and that they can play a role in retinal degeneration. This study aimed to profile synucleins in the retina of naturally aged mice and to correlate their patterns with specific retinal cells. With aging, we observed a decrease in the thickness of specific retinal layers, accompanied by an increase in glial reactivity. Moreover, the aSyn levels decreased, whereas bSyn increased with aging. The colocalization of both proteins was decreased in the inner plexiform layer (IPL) of the aged retina. gSyn presented an age-related decrease at the inner nuclear layer but was not significantly changed in the ganglion cell layer. The synaptic marker synaptophysin was shown to be preferentially colocalized with aSyn in the IPL with aging. At the same time, aSyn was found to exist at the presynaptic endings of bipolar cells and was affected by aging. Overall, this study suggests that physiological aging can be responsible for changes in the retinal tissue, implicating functional alterations that could affect synuclein family function.
The Vastus Lateralis Muscle Interstitium Proteome Changes after an Acute Nociception in Patients with Fibromyalgia Compared to Healthy Subjects—A Microdialysis Study
Authors: Ghafouri Bijar , Matikhan Daria , Christidis Nikolaos , Ernberg Malin , Kosek Eva , Mannerkorpi Kaisa , Gerdle Björn , Wåhlén Karin
Published: January 2023

Fibromyalgia (FM) is a complex disorder and a clinical challenge to diagnose and treat. Microdialysis is a valuable tool that has been used to investigate the interstitial proteins and metabolites of muscle in patients with fibromyalgia. The implantation of the catheter in the muscle causes acute tissue trauma and nociception. The aim of this study was to investigate acute proteome changes in the vastus lateralis muscle in women fibromyalgia patients (FM) and healthy subjects (CON). A further aim was to study if a 15-week resistance exercise program in FM had any influence on how chronic painful muscle responds to acute nociception. Twenty-six women patients with FM and twenty-eight CON were included in this study. A microdialysis catheter (100 kilo Dalton cut off, membrane 30 mm) was inserted in the vastus lateralis muscle, and samples were collected every 20 min. Subjects rated pain before catheter insertion, directly after, and every 20 min of sample collection. Dialysate samples from time points 0–120 were pooled and considered trauma samples due to the catheter insertion. The samples were analyzed with nano-liquid chromatography-tandem mass spectrometry (nLC-MS/MS). Advanced multivariate data analysis was used to investigate protein profile changes between the groups. Multivariate data analysis showed significant (CV-ANOVA p = 0.036) discrimination between FM and CON based on changes in 26 proteins. After the 15-week exercise intervention, the expression levels of the 15 proteins involved in muscle contraction, response to stimulus, stress, and immune system were increased to the same expression levels as in CON. In conclusion, this study shows that microdialysis, in combination with proteomics, can provide new insights into the interstitial proteome in the muscle of FM. In response to acute nociception, exercise may alter the innate reactivity in FM. Exercise may also modulate peripheral muscle proteins related to muscle contraction, stress, and immune response in patients with FM.
The Biology of Lysosomes: From Order to Disorder
Authors: Amaral Olga , Martins Mariana , Oliveira Ana Rita , Duarte Ana Joana , Mondragão-Rodrigues Inês , Macedo M. Fátima
Published: January 2023

Since its discovery in 1955, the understanding of the lysosome has continuously increased. Once considered a mere waste removal system, the lysosome is now recognised as a highly crucial cellular component for signalling and energy metabolism. This notable evolution raises the need for a summarized review of the lysosome’s biology. As such, throughout this article, we will be compiling the current knowledge regarding the lysosome’s biogenesis and functions. The comprehension of this organelle’s inner mechanisms is crucial to perceive how its impairment can give rise to lysosomal disease (LD). In this review, we highlight some examples of LD fine-tuned mechanisms that are already established, as well as others, which are still under investigation. Even though the understanding of the lysosome and its pathologies has expanded through the years, some of its intrinsic molecular aspects remain unknown. In order to illustrate the complexity of the lysosomal diseases we provide a few examples that have challenged the established single gene—single genetic disorder model. As such, we believe there is a strong need for further investigation of the exact abnormalities in the pathological pathways in lysosomal disease.
Antiparasitic Activity of Fluorophenyl-Substituted Pyrimido[1,2-a]benzimidazoles
Authors: Nasr Ibrahim S. Al , Koko Waleed S. , Khan Tariq A. , Schobert Rainer , Biersack Bernhard
Published: January 2023

A series of fourteen pyrimido[1,2-a]benzimidazole compounds was prepared by straightforward heterocyclic chemistry and oxidation methods. The new pyrimidobenzimidazole derivative 2a with a 3-fluorophenyl substituent was identified as a new antiparasitic compound showing excellent activities against Leishmania major parasites. 2a was highly active against L. major promastigotes and amastigotes with EC50 values in the nanomolar concentration range. Compound 3b was less active than 2a against L. major, but more active against Toxoplasma gondii with considerable selectivity. Hence, two promising and selective antiparasitic drug candidates 2a and 3b for the treatment of two parasitic diseases were identified, which can be prepared by green chemistry methods using simple one-pot reactions and oxidation procedures, respectively.
Early Saphenous Vein Graft Aneurysm Rupture: A Not So-Late Complication. Case Report and Comprehensive Literature Review
Authors: Mezzetti Eleonora , Maiese Aniello , Spina Federica , Del Duca Fabio , De Matteis Alessandra , Di Paolo Marco , La Russa Raffaele , Turillazzi Emanuela , Fineschi Vittorio
Published: January 2023

Background and Objectives: Saphenous vein graft (SVG) is a cardiac surgical practice used to create a cardiac bypass in cases of coronary artery obstruction. It consists of a surgical procedure that involves the creation of an aorto-coronary communication by a venous conduit (saphenous vein) to bypass coronary stenosis and allow cardiac revascularization. This practice can be affected by early and late complications. The most feared complication is graft aneurysm or pseudoaneurysm degeneration and rupture which are considered late complications. This paper presents a rare case of SVG aneurysmal rupture that occurred 24 h after surgery and a review of the literature to provide a general look at the state of knowledge. Materials and Methods: The systematic review was carried out using the guidelines according to the PRISMA method. Results: Cases of aneurysmal rupture have never been described prior to one month after surgery. The male sex and subjects under 45 are the most affected by this complication. Death occurs in less than half of the cases, being more frequent in young people. Performing a CT or angio-CT examination led to the diagnosis. Conclusions: It is impossible to estimate the implanted vessel’s quality, so postoperative follow-up is fundamental. Transesophageal ultrasound can be useful, and hematochemical tests are valuable early diagnostic tools, whrease CT and angio-CT can be useful even months after surgery. Forensic analysis should always perform an autopsy and graft histological examination.
Acute Spontaneous Lobar Cerebral Hemorrhages Present a Different Clinical Profile and a More Severe Early Prognosis than Deep Subcortical Intracerebral Hemorrhages—A Hospital-Based Stroke Registry Study
Authors: Mendiola Joana Maria Flaquer-Pérez de , Arboix Adrià , García-Eroles Luís , Sánchez-López Maria José
Published: January 2023

Acute spontaneous intracerebral hemorrhage (ICH) is the most severe stroke subtype, with a high risk of death, dependence, and dementia. Knowledge about the clinical profile and early outcomes of ICH patients with lobar versus deep subcortical brain topography remains limited. In this study, we investigated the effects of ICH topography on demographics, cerebrovascular risk factors, clinical characteristics, and early outcomes in a sample of 298 consecutive acute ICH patients (165 with lobar and 133 with subcortical hemorrhagic stroke) available in a single-center-based stroke registry over 24 years. The multiple logistic regression analysis shows that variables independently associated with lobar ICH were early seizures (OR 6.81, CI 95% 1.27–5.15), chronic liver disease (OR 4.55, 95% CI 1.03–20.15), hemianopia (OR 2.55, 95% CI 1.26–5.15), headaches (OR 1.90, 95% CI 1.90, 95% IC 1.06–3.41), alcohol abuse (>80 gr/day) (OR 0–10, 95% CI 0.02–0,53), hypertension (OR 0,41, 95% CI 0.23–0–70), sensory deficit (OR 0.43, 95% CI 0.25–0.75), and limb weakness (OR: 0.47, 95% CI 0.24–0.93). The in-hospital mortality was 26.7% for lobar and 16.5% for subcortical ICH. The study confirmed that the clinical spectrum, prognosis, and early mortality of patients with ICH depend on the site of bleeding, with a more severe early prognosis in lobar intracerebral hemorrhage.
Development of a Wearable Ultrasound Transducer for Sensing Muscle Activities in Assistive Robotics Applications
Authors: Xue Xiangming , Zhang Bohua , Moon Sunho , Xu Guo-Xuan , Huang Chih-Chung , Sharma Nitin , Jiang Xiaoning
Published: January 2023

Robotic prostheses and powered exoskeletons are novel assistive robotic devices for modern medicine. Muscle activity sensing plays an important role in controlling assistive robotics devices. Most devices measure the surface electromyography (sEMG) signal for myoelectric control. However, sEMG is an integrated signal from muscle activities. It is difficult to sense muscle movements in specific small regions, particularly at different depths. Alternatively, traditional ultrasound imaging has recently been proposed to monitor muscle activity due to its ability to directly visualize superficial and at-depth muscles. Despite their advantages, traditional ultrasound probes lack wearability. In this paper, a wearable ultrasound (US) transducer, based on lead zirconate titanate (PZT) and a polyimide substrate, was developed for a muscle activity sensing demonstration. The fabricated PZT-5A elements were arranged into a 4 × 4 array and then packaged in polydimethylsiloxane (PDMS). In vitro porcine tissue experiments were carried out by generating the muscle activities artificially, and the muscle movements were detected by the proposed wearable US transducer via muscle movement imaging. Experimental results showed that all 16 elements had very similar acoustic behaviors: the averaged central frequency, −6 dB bandwidth, and electrical impedance in water were 10.59 MHz, 37.69%, and 78.41 Ω, respectively. The in vitro study successfully demonstrated the capability of monitoring local muscle activity using the prototyped wearable transducer. The findings indicate that ultrasonic sensing may be an alternative to standardize myoelectric control for assistive robotics applications.
CRISPR/Cas12a-Assisted Dual Visualized Detection of SARS-CoV-2 on Frozen Shrimps
Authors: Qian Siwenjie , Chen Yanju , Wang Xiaofu , Wang Tingzhang , Che Yang , Wu Jian , Ye Zhangying , Xu Junfeng
Published: January 2023

Given the possibility that food contaminated with SARS-CoV-2 might become an infection source, there is an urgent need for us to develop a rapid and accurate nucleic acid detection method for SARS-CoV-2 in food to ensure food safety. Here, we propose a sensitive, specific, and reliable molecular detection method for SARS-CoV-2. It has a mechanism to control amplicon contamination. Swabs from spiked frozen shrimps were used as detection samples, which were processed by heating at 95 °C for 30 s. These preprocessed samples served as the templates for subsequent amplification. A colorimetric LAMP reaction was carried out to amplify both the SARS-CoV-2 target and the MS2 phage simultaneously in one tube. MS2 phage was detected by colorimetric LAMP as the internal control, while SARS-CoV-2 was detected with a CRISPR/Cas12a system. The fluorescence results could be visually detected with an ultraviolet lamp. Meanwhile, uracil was incorporated during the LAMP reaction to provide an amplicon contamination proof mechanism. This test could detect as low as 20 copies of SARS-CoV-2 in one reaction. Additionally, the detection could be finished in 45 min. The test only needs a heating block and an ultraviolet lamp, which shows the potential for field detection.
NIR Luminescent Oxygen-Sensing Nanoparticles for Continuous Glucose and Lactate Monitoring
Authors: Soundaram Jeevarathinam Ananthakrishnan , Saleem Waqas , Martin Nya , Hu Connie , McShane Michael J.
Published: January 2023

A highly sensitive, biocompatible, and scalable phosphorescent oxygen sensor formulation is designed and evaluated for use in continuous metabolite sensors for biological systems. Ethyl cellulose (EC) and polystyrene (PS) nanoparticles (NPs) stabilized with Pluronic F68 (PF 68), Polydimethylsiloxane-b-polyethyleneglycol methyl ether (PDMS-PEG), sodium dodecylsulfate (SDS), and cetyltimethylammonium bromide (CTAB) were prepared and studied. The resulting NPs with eight different surfactant–polymer matrix combinations were evaluated for physical properties, oxygen sensitivity, effect of changes in dispersion matrix, and cytotoxicity. The EC NPs exhibited a narrower size distribution and 40% higher sensitivity than PS, with Stern–Volmer constants (Ksv) 0.041–0.052 µM−1 for EC, compared to 0.029–0.034 µM−1 for PS. Notably, ethyl cellulose NPs protected with PF68 were selected as the preferred formulation, as they were not cytotoxic towards 3T3 fibroblasts and exhibited a wide phosphorescence lifetime response of >211.1 µs over 258–0 µM and ~100 µs over 2.58–0 µM oxygen, with a limit of detection (LoD) of oxygen in aqueous phase of 0.0016 µM. The EC-PF68 NPs were then efficiently encapsulated in alginate microparticles along with glucose oxidase (GOx) and catalase (CAT) to form phosphorescent nanoparticles-in-microparticle (NIMs) glucose sensing microdomains. The fabricated glucose sensors showed a sensitivity of 0.40 µs dL mg−1 with a dynamic phosphorescence lifetime range of 46.6–197.1 µs over 0–150 mg dL−1 glucose, with a glucose LoD of 18.3 mg dL−1 and maximum distinguishable concentration of 111.1 mg dL−1. Similarly, lactate sensors were prepared with NIMs microdomains containing lactate oxidase (LOx) and found to have a detection range of 0–14 mg dL−1 with LoD of 1.8 mg dL−1 and maximum concentration of 13.7 mg dL−1 with lactate sensitivity of 10.7 µs dL mg−1. Owing to its versatility, the proposed NIMs-based design can be extended to a wide range of metabolites and different oxygen-sensing dyes with different excitation wavelengths based on specific application.
Soil Microbial Fuel Cell Based Self-Powered Cathodic Biosensor for Sensitive Detection of Heavy Metals
Authors: Wang Shi-Hang , Wang Jian-Wei , Zhao Li-Ting , Abbas Syed , Yang Zhugen , Yong Yang-Chun
Published: January 2023

Soil microbial fuel cells (SMFCs) are an innovative device for soil-powered biosensors. However, the traditional SMFC sensors relied on anodic biosensing which might be unstable for long-term and continuous monitoring of toxic pollutants. Here, a carbon-felt-based cathodic SMFC biosensor was developed and applied for soil-powered long-term sensing of heavy metal ions. The SMFC-based biosensor generated output voltage about 400 mV with the external load of 1000 Ω. Upon the injection of metal ions, the voltage of the SMFC was increased sharply and quickly reached a stable output within 2~5 min. The metal ions of Cd2+, Zn2+, Pb2+, or Hg2+ ranging from 0.5 to 30 mg/L could be quantified by using this SMFC biosensor. As the anode was immersed in the deep soil, this SMFC-based biosensor was able to monitor efficiently for four months under repeated metal ions detection without significant decrease on the output voltage. This finding demonstrated the clear potential of the cathodic SMFC biosensor, which can be further implemented as a low-cost self-powered biosensor.
Imaging and Hemodynamic Characteristics of Vulnerable Carotid Plaques and Artificial Intelligence Applications in Plaque Classification and Segmentation
Authors: Han Na , Ma Yurong , Li Yan , Zheng Yu , Wu Chuang , Gan Tiejun , Li Min , Ma Laiyang , Zhang Jing
Published: January 2023

Stroke is a massive public health problem. The rupture of vulnerable carotid atherosclerotic plaques is the most common cause of acute ischemic stroke (AIS) across the world. Currently, vessel wall high-resolution magnetic resonance imaging (VW-HRMRI) is the most appropriate and cost-effective imaging technique to characterize carotid plaque vulnerability and plays an important role in promoting early diagnosis and guiding aggressive clinical therapy to reduce the risk of plaque rupture and AIS. In recent years, great progress has been made in imaging research on vulnerable carotid plaques. This review summarizes developments in the imaging and hemodynamic characteristics of vulnerable carotid plaques on the basis of VW-HRMRI and four-dimensional (4D) flow MRI, and it discusses the relationship between these characteristics and ischemic stroke. In addition, the applications of artificial intelligence in plaque classification and segmentation are reviewed.
Effects of Voice and Biographic Data on Face Encoding
Authors: Karlsson Thilda , Schaefer Heidi , Barton Jason J. S. , Corrow Sherryse L.
Published: January 2023

There are various perceptual and informational cues for recognizing people. How these interact in the recognition process is of interest. Our goal was to determine if the encoding of faces was enhanced by the concurrent presence of a voice, biographic data, or both. Using a between-subject design, four groups of 10 subjects learned the identities of 24 faces seen in video-clips. Half of the faces were seen only with their names, while the other half had additional information. For the first group this was the person’s voice, for the second, it was biographic data, and for the third, both voice and biographic data. In a fourth control group, the additional information was the voice of a generic narrator relating non-biographic information. In the retrieval phase, subjects performed a familiarity task and then a face-to-name identification task with dynamic faces alone. Our results consistently showed no benefit to face encoding with additional information, for either the familiarity or identification task. Tests for equivalency indicated that facilitative effects of a voice or biographic data on face encoding were not likely to exceed 3% in accuracy. We conclude that face encoding is minimally influenced by cross-modal information from voices or biographic data.
Resolvin D2 Reduces Chronic Neuropathic Pain and Bone Cancer Pain via Spinal Inhibition of IL-17 Secretion, CXCL1 Release and Astrocyte Activation in Mice
Authors: Pang Jun , Xin Pengfei , Kong Ying , Wang Zhe , Wang Xiaopeng
Published: January 2023

Chronic pain burdens patients and healthcare systems worldwide. Pain control remains urgently required. IL-17 (interleukin-17)-mediated neuroinflammation is of unique importance in spinal nociceptive transduction in pathological pain development. Recently, resolvin D2 (RvD2), as a bioactive, specialized pro-resolving mediator derived from docosahexaenoic acid, exhibits potent resolution of inflammation in several neurological disorders. This preclinical study evaluates the therapeutic potential and underlying targets of RvD2 in two mouse models of chronic pain, including sciatic nerve ligation-caused neuropathic pain and sarcoma-caused bone cancer pain. Herein, we report that repetitive injections of RvD2 (intrathecal, 500 ng) reduce the initiation of mechanical allodynia and heat hyperalgesia following sciatic nerve damage and bone cancer. Single exposure to RvD2 (intrathecal, 500 ng) attenuates the established neuropathic pain and bone cancer pain. Furthermore, systemic RvD2 (intravenous, 5 μg) therapy is effective in attenuating chronic pain behaviors. Strikingly, RvD2 treatment suppresses spinal IL-17 overexpression, chemokine CXCL1 release and astrocyte activation in mice undergoing sciatic nerve trauma and bone cancer. Pharmacological neutralization of IL-17 ameliorates chronic neuropathic pain and persistent bone cancer pain, as well as reducing spinal CXCL1 release. Recombinant IL-17-evoked acute pain behaviors and spinal CXCL1 release are mitigated after RvD2 administration. In addition, RvD2 treatment dampens exogenous CXCL1-caused transient pain phenotypes. Overall, these current findings identify that RvD2 therapy is effective against the initiation and persistence of long-lasting neuropathic pain and bone cancer pain, which may be through spinal down-modulation of IL-17 secretion, CXCL1 release and astrocyte activation.
Sex Differences in the Level of Homocysteine in Alzheimer’s Disease and Parkinson’s Disease Patients: A Meta-Analysis
Authors: Nguyen V. Phu , Collins Andrila E. , Hickey Jordan P. , Pfeifer Julia A. , Kalisch Bettina E.
Published: January 2023

Although recent studies suggest homocysteine (Hcy) is an independent risk factor for neurodegenerative disorders, little is known about sex differences in the levels of Hcy. In this study, we conducted a comparative meta-analysis to investigate sex differences in the levels of Hcy in both Alzheimer’s disease (AD) and Parkinson’s disease (PD) patients. Reports of Hcy stratified by sex in both AD and PD patients were obtained from electronic databases. From the initial 1595 records, 921 were assessed for eligibility, of which 16 sufficiently reported sex differences. Standardized mean difference (SMDs) using random effects together with tests of heterogeneity and quality assessment were applied in this meta-analysis. Data from 3082 diagnosed patients (1162 males and 1920 females) were included. There were statistically significant differences in the levels of Hcy between sexes in AD and PD patients, with an SMD of 0.291 [0.17, 0.41], p < 0.05, 95% CI, with higher Hcy levels detected in males. Subgroup comparisons did not find a statistically significant difference in the levels of Hcy between AD and PD patients. The overall risk of bias for the analyzed studies was low, with some moderate risk of bias across select domains. This meta-analysis determined that compared to females, males with either AD or PD have higher levels of Hcy. These findings suggest that Hcy could be a useful biomarker for predicting neurodegenerative diseases in males; however, further studies are needed to confirm the clinical utility of this suggestion.
Screening Children’s Intellectual Disabilities with Phonetic Features, Facial Phenotype and Craniofacial Variability Index
Authors: Chen Yuhe , Ma Simeng , Yang Xiaoyu , Liu Dujuan , Yang Jun
Published: January 2023

Background: Intellectual Disability (ID) is a kind of developmental deficiency syndrome caused by congenital diseases or postnatal events. This syndrome could be intervened as soon as possible if its early screening was efficient, which may improve the condition of patients and enhance their self-care ability. The early screening of ID is always achieved by clinical interview, which needs in-depth participation of medical professionals and related medical resources. Methods: A new method for screening ID has been proposed by analyzing the facial phenotype and phonetic characteristic of young subjects. First, the geometric features of subjects’ faces and phonetic features of subjects’ voice are extracted from interview videos, then craniofacial variability index (CVI) is calculated with the geometric features and the risk of ID is given with the measure of CVI. Furthermore, machine learning algorithms are utilized to establish a method for further screening ID based on facial features and phonetic features. Results: The proposed method using three feature sets, including geometric features, CVI features and phonetic features was evaluated. The best performance of accuracy was closer to 80%. Conclusions: The results using the three feature sets revealed that the proposed method may be applied in a clinical setting in the future after continuous improvement.
Predictive Value of CT Perfusion in Hemorrhagic Transformation after Acute Ischemic Stroke: A Systematic Review and Meta-Analysis
Authors: Xu Jie , Dai Fangyu , Wang Binda , Wang Yiming , Li Jiaqian , Pan Lulan , Liu Jingjing , Liu Haipeng , He Songbin
Published: January 2023

Background: Existing studies indicate that some computed tomography perfusion (CTP) parameters may predict hemorrhagic transformation (HT) after acute ischemic stroke (AIS), but there is an inconsistency in the conclusions alongside a lack of comprehensive comparison. Objective: To comprehensively evaluate the predictive value of CTP parameters in HT after AIS. Data sources: A systematical literature review of existing studies was conducted up to 1st October 2022 in six mainstream databases that included original data on the CTP parameters of HT and non-HT groups or on the diagnostic performance of relative cerebral blood flow (rCBF), relative permeability-surface area product (rPS), or relative cerebral blood volume (rCBV) in patients with AIS that completed CTP within 24 h of onset. Data Synthesis: Eighteen observational studies were included. HT and non-HT groups had statistically significant differences in CBF, CBV, PS, rCBF, rCBV, and rPS (p < 0.05 for all). The hierarchical summary receiver operating characteristic (HSROC) revealed that rCBF (area under the curve (AUC) = 0.9), rPS (AUC = 0.89), and rCBV (AUC = 0.85) had moderate diagnostic performances in predicting HT. The pooled sensitivity and specificity of rCBF were 0.85 (95% CI, 0.75–0.91) and 0.83 (95% CI, 0.63–0.94), respectively. Conclusions: rCBF, rPS, and rCBV had moderate diagnostic performances in predicting HT, and rCBF had the best pooled sensitivity and specificity.
Anthropometric Profile and Physical Activity Level as Predictors of Postural Balance in Overweight and Obese Children
Authors: Guzmán-Muñoz Eduardo , Mendez-Rebolledo Guillermo , Núñez-Espinosa Cristián , Valdés-Badilla Pablo , Monsalves-Álvarez Matías , Delgado-Floody Pedro , Herrera-Valenzuela Tomás
Published: January 2023

Overweightness and obesity can negatively influence many activities, including postural balance and locomotion, increasing predisposition to injury and risk of falls due to limitations on the biomechanics of daily living. The present study aimed to determine the influence of the anthropometric profile and physical activity level (PAL) on the postural balance of overweight and obese children. The sample included 387 schoolchildren (216 boys and 171 girls). The variables of the anthropometric profile studied were body mass, biped height, BMI, waist circumference, waist-to-hip ratio (WHR), summation of folds, body composition, and somatotype. PAL was measured using the PAQ-C questionnaire. Static and dynamic postural balance were measured through an open-eye (OE) and closed-eye (CE) posturographic test and the SEBTm, respectively. For static balance, the significant models were for mediolateral velocity (R2 = 0.42 in OA; R2 = 0.24 in OC), anteroposterior velocity (R2 = 0.21 in OA; R2 = 0.27 in OC), and mean velocity (R2 = 0.27 in OA; R2 = 0.46 in OC), where the predictors of low performance were younger age, male sex, overweight/obese nutritional status, greater thickness of skin folds, less tendency to mesomorphy, and greater fat mass. On the other hand, for dynamic postural balance, the significant models were observed in the previous direction (R2 = 0.39), posteromedial (R2 = 0.57), and posterolateral (R2 = 0.56), where the variables that predict a low performance were low PAL, overweight/obese nutritional status, and high WHR. Overweight and obese children presented a deficit in static and dynamic postural balance, enhanced by variables such as gender, age, PAL, and anthropometric characteristics related to adiposity.
The Influence of Image Realism of Digital Endorsers on the Purchase Intention of Gift Products for the Elderly
Authors: Wang Xiaoyi , Qiu Xingyi
Published: January 2023

Digital endorsers are already utilized extensively in various businesses. The major objective of the current study was to find out the impact of image realism of digital endorsers on the purchase intention of gift products for the elderly. We investigated this issue through three online experiments. Study 1 (n = 205) found that cartoon digital endorsers (vs. realistic digital endorsers) generate higher purchase intention for the product. Study 2 (n = 175) showed that perceived social value plays a mediating role in the relationship between the image realism of digital endorsers and purchase intention. Study 3 (n = 127) demonstrated the moderating role of information framing in the relationship between the image realism of digital endorsers and purchase intention. In all, our research extends the previous literature on digital human endorsements and advertising of elderly products and provides several managerial implications for consumers and marketers.
More-Than-Human Perspective in Indigenous Cultures: Holistic Systems Informing Computational Models in Architecture, Urban and Landscape Design towards the Post-Anthropocene Epoch
Authors: Zavoleas Yannis , Stevens Peter , Johnstone Jenny , Davidová Marie
Published: January 2023

By studying Aboriginal maps, this speculative research discusses world heritage concepts about land and merges them into western urban contexts. Assumptions concerning spatial allocation and demarcation such as boundaries, divisions and geometric patterns are being contested by ideas pertaining to Indigenous narratives expressing holistic views about community, and the ecosystem as integrated components of broader organisations. First, this paper introduces principles of the Indigenous culture spurring viable land management by shared, equal and inclusive schemes as ones that also respond to global socio-environmental challenges. Alternative strategies are being considered relating to the soft demarcation of distinct areas understood as malleable aggregates merging with each other and with the landscape’s topological features, with reference to the Aboriginal culture. The techniques being proposed are further compared with original approaches in architecture and urban design developed since late modernism, challenging enduring practices. Seen next to each other, these models of thought are suggestive of a paradigm shift by which architecture reinforces deeper connections with the intellectual, sociocultural, and natural resources of the greater cosmos. Furthermore, as these ideas are propelled by computing, they lead towards the dynamic linking of analysis with the design results producing all-sustainable structures that are widely applicable, as architecture’s contribution to the current socio-scientific discourse on holistic approaches with a more-than-human perspective.
Parametric Study on Built-Up Cold-Formed Steel Beams with Web Openings Connected by Spot Welding
Authors: Cristian Antonio Andrei , Ungureanu Viorel
Published: January 2023

This paper presents a numerical parametric study on cold-formed steel built-up beams subjected to bending. The cold-formed steel built-up elements are efficient structural elements that are easy to assemble during the construction process, ensuring material savings and potential for standardization, thus making them more suitable for mass production. A new technological solution for built-up steel beams with webs made of corrugated steel sheets and flanges made of cold-formed steel profiles, assembled through two welding techniques, was proposed within the well-formed research project. This solution can be used as a component of single or low-rise multi-story frames, purlins, or secondary beams. The experimental program investigated seven full-scale beams, two of which have web openings. The web openings were introduced for the case when these members are used as secondary beams in floor systems. The paper investigates these types of beams with web openings using parametric numerical analyses. A numerical model validated against experimental tests was proposed to carry out a parametric study through nonlinear finite element analysis, considering initial imperfections and considering the strain-hardening characteristics of the steel components. The influence of different components was analyzed through the parametric study. Beams with three lengths were studied, i.e., (1) 6000 mm, (2) 7500 mm, and (3) 9000 mm. In total, 61 simulations were found. The article highlights the parameters that contribute significantly to the stiffness and capacity of the built-up cold-formed steel elements. The weakest component was concluded to be the thickness of the corrugated web, while the presence of the stiffened web opening reduced the bearing capacity by approximately 5–10%.
Energy Evolution and Damage Characteristics of Rock Materials under Different Cyclic Loading and Unloading Paths
Authors: Sun Bing , Yang Haowei , Fan Junwei , Liu Xiling , Zeng Sheng
Published: January 2023

In order to study the deformation and failure characteristics of rocks under different cyclic loading and unloading paths, three stress path tests were conducted, and acoustic emission (AE) monitoring was conducted simultaneously. The mechanical characteristics and AE characteristics under different stress paths were analyzed, and the influences of the different stress paths on the energy dissipation and deformation damage were investigated. The law of energy evolution considering viscoelasticity under different stress paths was obtained. The concept of ultimate damage energy and its calculation method was proposed. The results show that the “hardening effect” of sandstone and granite under the constant lower limit (CLLCL) is the most significant in maximizing the mechanical property. The CLLCL imparts a stronger elastic property to rocks than the variable lower limit (VLLCL) does, while the VLLCL causes more damage to rocks than the CLLCL. A significant linear relationship between the proportion of damage energy and the proportion of elastic energy was discovered. Based on this linear relationship, the ultimate damage energy can be calculated for sandstone and granite. The evolution of the damage variable based on damage energy was compatible with the real damage condition, which validates the ultimate damage energy calculation method. The research results lay a theoretical foundation for the design and construction of geotechnical engineering.
Introduction of an Integrated System for Measuring the BSC and DEA-Based Performance of Social Systems Using Uncontrollable Factors: A Case Study of Shahriar Municipalities
Authors: Chaharlang Yusof , Soleimani Hamed , Mehdizadeh Esmaeil , Alinezhad Alireza
Published: January 2023

Today, achieving managerial success and ongoing improvement depend greatly on the performance evaluation of service and economic units. On the other hand, we are aware that strategy is the primary driver of an organization’s long-term development and that, even if the right strategies are adopted, this effort will be ineffective if the strategy is not effectively executed. A balanced scorecard is an essential tool for strategy implementation. The network data envelopment analysis model, on the other hand, is recommended for the relative analysis and assessment of decision-making units in evaluating and improving the organizations’ performance with multiple inputs and outputs. The four components of the balanced scorecard are financial (retrospective indicators), and processes, customers, and learning and growth (prospective indicators). According to investigations, in addition to the four previously mentioned fields, a balanced scorecard for urban services (municipality) also encompasses the social responsibility field. In order to design performance evaluation indicators, this article attempted to use BSC while also attempting to evaluate performance using DEA. The Shahriar city municipal units of Ferdowsieh, Vahidie, Sabashahr, Shahedshahr, Andisheh, Baghestan, and Shahryar have all adopted this hybrid model. The findings indicate that the municipality of Andisheh city is efficient, while the remaining municipalities are inefficient. Nevertheless, the Baghestan municipality performs well and is effective in terms of social responsibilities.
Behavior of Cross-Laminated Timber Panels Made from Fibre-Managed Eucalyptus nitens under Short-Term Serviceability Loads
Authors: Liang Yingwei , Taoum Assaad , Kotlarewski Nathan , Chan Andrew , Holloway Damien
Published: January 2023

In this study, the preliminary serviceability performance of cross-laminated timber (CLT) panels constructed from fibre-managed Eucalyptus nitens (E. nitens) was investigated via bending and vibration tests. Linear four-point bending tests were performed to determine the stiffness and deflection of all CLT panels under serviceability loads. The dynamic response of CLT panels was tested using a basketball and an accelerometer. The fundamental natural frequencies of all tested panels were above the minimum frequency limit (8 Hz) when extrapolated to spans of up to 4.4 m. The configurations of E. nitens CLT panels were based on different modulus of elasticity (MOE) values for each board. Using higher MOE timber boards as the top and bottom layers can significantly increase the serviceability performance of both bending and vibration tests. The same experiments were carried out on two CLT panels made of strength class C24 Spruce-Pine-Fir to compare the serviceability performance of E. nitens CLT. The results demonstrated that E. nitens is a reliable resource for CLT manufacturing, and exhibits better serviceability performance compared to Spruce CLT. This provides more sustainable options for a species traditionally destined for pulp.