bluuebunny/crossref_metadata_2025 huggingface dataset:
Incidences of Rhipicephalus (Boophilus) microplus (Canestrini, 1888) Transmitted Pathogens in Cattle in West Africa
Authors: Toure Alassane , Sanogo Moussa , Sghiri Abdelmalek , Sahibi Hamid
Published: June 2022

Context and Purpose

In a context of recent introduction of Rhipicephalus (Boophilus) microplus tick species in West Africa, the purpose of the authors is to estimate incidence density of cattle babesiosis either caused by Babesia bigemina or Babesia bovis, and cattle anaplasmosis. Incidence density represents how quickly a disease or a condition is occurring amongst a group of individuals at risk.

Methods

The longitudinal and prospective study design took place in south, centre, east, west and north of Côte d’Ivoire. Cattle have been followed for 12 months. At the end of each month, each animal has been RT-PCR tested for new infection by Babesia bovis, Babesia bigemina, and PCR–RFLP tested for new infection by Anaplasma marginale.

Results

Findings show for the study area that incidence densities of Babesia bovis, Babesia bigemina and Anaplasma marginale infections in Côte d’Ivoire are, respectively, 15.3 new infections [95% CI 13.1–17.88] per 100 cattle, 32.2 new infections [95% CI 28.5–36.3] per 100 cattle, and 25.9 new infections [95% CI 22.5–29.6] per 100 cattle.

Conclusion

Finally, there is increasing of infection incidence density following the region distance from the coast or elevation.
Evaluation of Ongoing Crisis Intervention Team (CIT) Training for Law Enforcement Using the ECHO Model
Authors: Crisanti Annette S. , Fairfax-Columbo Jaymes , Duran Danielle , Rosenbaum Nils A. , Melendrez Ben , Trujillo Isaac , Earheart Jennifer A. , Tinney Matthew
Published: June 2022

Crisis Intervention Team (CIT) training aims to improve law enforcement officers’ (LEOs) ability to safely intervene in calls for service involving individuals with mental illness, as well as to increase LEOs’ ability to link these individuals to mental health services and divert them from the criminal justice system. However, most CIT training is delivered as a stand-alone class, and continuing education in CIT principles and best practices is limited. To address this problem, the Albuquerque Police Department, in partnership with the Department of Psychiatry and Behavioral Sciences at the University of New Mexico, developed CIT ECHO to provide continuing education in CIT best practices. The authors evaluated 113 weekly CIT ECHO sessions targeting LEOs in New Mexico, offered between 2017 and 2020. LEOs electronically completed a post-session survey after each didactic; additionally, a targeted follow-up survey was distributed to LEOs participating in at least three sessions. Surveys measured impact of CIT ECHO on knowledge, self-efficacy, and attitudes towards individuals with mental illness involved in the criminal justice system. After participating in CIT ECHO, LEOs reported increases in knowledge of didactic content and that they felt comfortable applying didactic content on the job. LEOs also evidenced positive attitudinal shifts towards individuals with mental illness and criminal justice involvement. Continuing education in CIT best practices appears to increase LEOs’ knowledge base and comfort in working with individuals with mental illness and criminal justice involvement, as well as results in positive attitudinal shifts towards this population.
Infectious Pepper Mild Mottle Virus and Human Adenoviruses as Viral Indices in Sewage and Water Samples
Authors: Rashed Mohammed Kamal , El-Senousy Waled Morsy , Sayed ElSayed Tarek Abd ElSalam , AlKhazindar Maha
Published: June 2022

The objective of this study was to compare human adenoviruses (HAdVs) genome and infectivity, polyomaviruses (JC and BK) genome (JCPyVs) and (BKPyVs), Pepper Mild Mottle Virus (PMMoV) genome and infectivity, and infectious bacteriophages as viral indices for sewage and water samples. One hundred and forty-four samples were collected from inlets and outlets of water and wastewater treatment plants (WTPs), and WWTPs within Greater Cairo from October 2015 till March 2017. Two methods of viral concentration [Aluminium hydroxide (Al(OH)3) precipitation method and adsorption-elution technique followed by organic flocculation method] were compared to determine which of them was the best method to concentrate viruses from sewage and water. Although samples with only one litre volume were concentrated using Al(OH)3 precipitation method and the same samples with larger volumes (5–20 L) were concentrated using the adsorption-elution technique followed by the organic flocculation method, a non-significant difference was observed between the efficiency of the two methods in all types of samples except for the drinking water samples. Based on the qualitative prevalence of studied viruses in water and wastewater samples, the number of genome copies and infectious units in the same samples, resistance to treatment processes in water and wastewater treatment plants, higher frequency of both adenoviruses and PMMoV genomes as candidate viral indices in treated sewage and drinking water was observed. The problem of having a viral genome as indices of viral pollution is that it does not express the recent viral pollution because of the longer survivability of the viral genome than the infectious units in water and wastewater. Both infectious adenovirus and infectious phiX174 bacteriophage virus showed similar efficiencies as indices for viral pollution in drinking water and treated sewage samples. On the other hand, qualitative detection of infectious PMMoV failed to express efficiently the presence/absence of infectious enteric viruses in drinking water samples. Infectious adenoviruses and infectious bacteriophage phiX174 virus may be better candidates than adenoviruses genome, polyomaviruses genome, and PMMoV genome and infectivity as viral indices for water and wastewater.
On Effective Campus Attack Response: Insights from Agent-Based Simulation for Improving Emergency Information Sharing System Design and Response Strategy
Authors: Beattie Steven , Xi Jing Yang Sunny , Chan Wai Kin Victor
Published: June 2022

Information sharing systems are a critical component of emergency response—especially in campus attack situations that unfold very rapidly. The design of effective information sharing systems is often difficult, however, due to a lack of data on these assault events. This work takes an agent-based approach to simulate three campus emergency information sharing system design alternatives in the context of a college campus knife attack, and incorporates data from on-campus student surveys and parameter tuning experiments. Alternatives are evaluated according to: (1) improved student attack response outcomes; and (2) effective institutional response to the attack. The results confirm that increased awareness supports rapid emergency reporting, but an important gap exists between students’ awareness and their ability to respond effectively, which depends on a number of campus-specific factors. A strong positive impact is seen from safe and efficient information sharing with authorities. This impact depends largely on reporting system implementation qualities, as opposed to campus-specific factors. On a campus in China, WeChat was used as a basis for messaging models. The simulation results show a 9% drop in casualties and a 22% faster police response time from a text-based reporting system using “base” WeChat features instead of traditional phone reporting. Our results also project a 30% drop in casualties and 52% faster police response time using a system designed around a WeChat Mini Program or stand-alone campus emergency reporting app. These outcomes suggest a number of recommendations for improving outdated campus emergency information-sharing systems and response strategies.
Imperceptible black-box waveform-level adversarial attack towards automatic speaker recognition
Authors: Zhang Xingyu , Zhang Xiongwei , Sun Meng , Zou Xia , Chen Kejiang , Yu Nenghai
Published: June 2022

Automatic speaker recognition is an important biometric authentication approach with emerging applications. However, recent research has shown its vulnerability on adversarial attacks. In this paper, we propose a new type of adversarial examples by generating imperceptible adversarial samples for targeted attacks on black-box systems of automatic speaker recognition. Waveform samples are created directly by solving an optimization problem with waveform inputs and outputs, which is more realistic in real-life scenario. Inspired by auditory masking, a regularization term adapting to the energy of speech waveform is proposed for generating imperceptible adversarial perturbations. The optimization problems are subsequently solved by differential evolution algorithm in a black-box manner which does not require any knowledge on the inner configuration of the recognition systems. Experiments conducted on commonly used data sets, LibriSpeech and VoxCeleb, show that the proposed methods have successfully performed targeted attacks on state-of-the-art speaker recognition systems while being imperceptible to human listeners. Given the high SNR and PESQ scores of the yielded adversarial samples, the proposed methods deteriorate less on the quality of the original signals than several recently proposed methods, which justifies the imperceptibility of adversarial samples.
Network loss following the 2016 Presidential Election among LGBTQ+ adults
Authors: Facciani Matthew , McKay Tara
Published: June 2022

Growing levels of political polarization in the United States have been associated with political homogeneity in the personal networks of American adults. The 2016 Presidential Election in the United States was a polarizing event that may have caused further loss of connections to alters who had different politics. Kinship may protect against loss of politically different ties. Additionally, loss of ties with different political views may be particularly pronounced among LGBTQ+ people as they are more likely to be impacted by public policy decisions compared to their heterosexual counterparts. We analyzed two waves of the University of California, Berkeley Social Networks Study's (UCNets) Main Sample and LGBTQ+ Oversample of older adults that occurred in 2015 and 2017, which provided an opportunity to assess alter loss after the 2016 Presidential Election. When evaluating all adults, we found that politically different alters were more likely to reflect kin ties than partner or friend ties. We also found that politically different kin are less likely to be dropped suggesting that kinship acts as a moderating effect of different political views on alter loss. LGBTQ+ respondents were more likely to drop kin alters with different political views than their cisgender heterosexual counterparts. We discuss the implications these results have for political polarization interventions as well as the social networks impact politics can have on LGBTQ+ individuals.
‘I somehow had the Feeling that I did not belong there’—Experiences of Gay and Lesbian Recreational Athletes in German Sports Clubs
Authors: Müller Johannes , Böhlke Nicola
Published: June 2022

There is little empirical research on the sport experiences of gay and lesbian recreational athletes in Germany and their existence and needs within organized, non-professional sports have largely been ignored. Based on twelve in-depth interviews with self-identified male gay and female lesbian adults, this paper explores how queer recreational athletes experience sport in German sports clubs and which particular challenges or discriminatory situations they are confronted with. Findings show that study participants do not experience much discrimination on an explicit level in the sports clubs. Nevertheless, many respondents try to hide their sexuality in the sports context to prevent possible discrimination and questions about their sexuality. After all, it is mainly the discussion about and reduction of their sexuality that is being experienced as problematic. Five main stressors have been identified: (1) the necessity of an outing, (2) the sports club typical mode of communication, (3) the heteronormative pre-structuring of the sports, (4) the feeling of otherness and the assigned special role, (5) the implicit fear of discrimination. The findings point to the need for increased reflection on and reduction of heteronormative structures in German sports clubs.
Quantum neural network autoencoder and classifier applied to an industrial case study
Authors: Mangini Stefano , Marruzzo Alessia , Piantanida Marco , Gerace Dario , Bajoni Daniele , Macchiavello Chiara
Published: June 2022

Quantum computing technologies are in the process of moving from academic research to real industrial applications, with the first hints of quantum advantage demonstrated in recent months. In these early practical uses of quantum computers, it is relevant to develop algorithms that are useful for actual industrial processes. In this work, we propose a quantum pipeline, comprising a quantum autoencoder followed by a quantum classifier, which are used to first compress and then label classical data coming from a separator, i.e., a machine used in one of Eni’s Oil Treatment Plants. This work represents one of the first attempts to integrate quantum computing procedures in a real-case scenario of an industrial pipeline, in particular using actual data coming from physical machines, rather than pedagogical data from benchmark datasets.
Analysis of User Behaviour While Interpreting Spatial Patterns in Point Data Sets
Authors: Knura Martin , Schiewe Jochen
Published: June 2022

Volunteered geographic information is often generated as voluminous point data, leading to geometric and thematic clutter when presented on maps. To solve these clutter problems, cartography provides various point generalization operations such as aggregation, simplification or selection. While these operations reduce the total number of points and therefore improve the readability, information preservation could be harmed when specific spatial patterns disappear through the generalization process, possibly leading to false interpretations. However, sets of map generalization constraints that maintain spatial pattern characteristics of point data are still missing. To define constraints that support synoptic interpretation tasks, user behaviour while solving these tasks has to be analysed first. We conduct a study where participants have to perform such interpretation tasks, using a new method that combines think-aloud interviews and techniques from visual analytics. We reveal that the point density of a dataset has the biggest impact on the user behaviour and the respective task-solving strategy, independently from the actual task type executed. Furthermore, our results show that the graphical map complexity only has a minor impact on the user behaviour, and there is no evidence that point data cardinality influences task execution and the solution-finding strategies.
The Sound Current Tradition
Authors: Lane David Christopher
Published: June 2022

The practice of listening to subtle, inner sounds during meditation to concentrate and elevate the mind has a long history in various religions around the world, including Islam, Christianity, Hinduism, Buddhism, Jainism, and Sikhism. Today there are a number of new religious movements that have made listening to the inner sound current a cornerstone of their teachings. These groups include the Radhasoamis, the Divine Light Mission, Eckankar, the Movement of Spiritual Inner Awareness (MSIA), MasterPath, the Sawan-Kirpal Mission, Quan Yin/Ching Hai, Manavta Mandir, ISHA, and a number of others. In this study we provide a historical and comprehensive overview of these movements and how they have incorporated listening to the inner sound as part of their spiritual discipline. We are particularly interested in the distinctive and nuanced ways that each group teaches how to listen to the inner sound current and how they interpret it in their own unique theologies.
Understanding How Science Explains the World
Authors: McCain Kevin
Published: June 2022

All people desire to know. We want to not only know what has happened, but also why it happened, how it happened, whether it will happen again, whether it can be made to happen or not happen, and so on. In short, what we want are explanations. Asking and answering explanatory questions lies at the very heart of scientific practice. The primary aim of this book is to help readers understand how science explains the world. This book explores the nature and contours of scientific explanation, how such explanations are evaluated, as well as how they lead to knowledge and understanding. As well as providing an introduction to scientific explanation, it also tackles misconceptions and misunderstandings, while remaining accessible to a general audience with little or no prior philosophical training.
Creation and impact of containment units with high-risk zones during the coronavirus disease 2019 (COVID-19) pandemic
Authors: Schnell Natalie A. , Brewer Brooke , Young Kimberly , Brown Turkeisha S. , Carson Shannon S. , Culp Loc , Culbreth Cynthia , DiBiase Lauren M. , Fischer William A. , Schultz Katherine M. , Sickbert-Bennett Emily E. , Stancill Lisa , Weber David J. , Wolak Erica , Teal Lisa J.
Published: June 2022

Background:

The rapid spread of coronavirus disease 2019 (COVID-19) required swift preparation to protect healthcare personnel (HCP) and patients, especially considering shortages of personal protective equipment (PPE). Due to the lack of a pre-existing biocontainment unit, we needed to develop a novel approach to placing patients in isolation cohorts while working with the pre-existing physical space.

Objectives:

To prevent disease transmission to non–COVID-19 patients and HCP caring for COVID-19 patients, to optimize PPE usage, and to provide a comfortable and safe working environment.

Methods:

An interdisciplinary workgroup developed a combination of approaches to convert existing spaces into COVID-19 containment units with high-risk zones (HRZs). We developed standard workflow and visual management in conjunction with updated staff training and workflows. The infection prevention team created PPE standard practices for ease of use, conservation, and staff safety.

Results:

The interventions resulted in 1 possible case of patient-to-HCP transmission and zero cases of patient-to-patient transmission. PPE usage decreased with the HRZ model while maintaining a safe environment of care. Staff on the COVID-19 units were extremely satisfied with PPE availability (76.7%) and efforts to protect them from COVID-19 (72.7%). Moreover, 54.8% of HCP working in the COVID-19 unit agreed that PPE monitors played an essential role in staff safety.

Conclusions:

The HRZ model of containment unit is an effective method to prevent the spread of COVID-19 with several benefits. It is easily implemented and scaled to accommodate census changes. Our experience suggests that other institutions do not need to modify existing physical structures to create similarly protective spaces.
Reaffirmation and Development of International Humanitarian Law Applicable in Armed Conflicts - Conference of Government Experts
Authors: Naville M. A. , Pictet M. J. , Pilloud C. , Wilhelm R.-J.
Published: June 2022

In our previous issue, we reported on the opening meeting on 24 May 1971 of the Conference of Government Experts, convened in Geneva by the ICRC, and at the same time we gave the texts of the opening speeches which revealed how important the meeting was. The Conference finished on 11 June and we now quote below the message of the President of the ICRC at the closing session, as well as three papers delivered outlining the work of the Conference.
Sinophone Classicism: Chineseness as Temporal and Mnemonic Experience in the Digital Era
Authors: Yang Zhiyi
Published: June 2022

In recent decades, highly heterogeneous literary and artistic articulations harking back to China's classical past have gained increasing currency in the global Sinophone space and cyberspace. Instead of dismissing them as “fetishisms” or authenticating them as “Chinese traditions,” I propose “Sinophone classicism” as a new critical expression for conceptualizing this diverse array of articulations. It refers to the appropriation, redeployment, and reconfiguration of cultural memories evoking Chinese aesthetic and intellectual traditions for local, contemporary, and vernacular uses, by agents identified or self-identified as Chinese. This essay proposes a subjective, intimate, and reflexive way to experience an individual's culturally acquired “Chineseness” that is temporal, mnemonic, and often mediated by digital media. It joins recent scholarly efforts to dismantle the view of “Chinese modernity” as a monocentric and homogenous experience by refocusing on classicism as a kind of “antimodern modernism.” It also joins the post-Eurocentric turn in global academia by hinting at a future of “global classicisms.”
Postcolonial paths of pop: a suburban psychogeography of George Michael and Wham!
Authors: Negus Keith , Sledmere Adrian
Published: June 2022

This article draws on psychogeography to explore suburban Bushey, Hertfordshire (UK), where the popular music duo Wham! formed and created the foundation for the later career of George Michael. It locates this neglected cultural narrative within the context of parental postcolonial and metropolitan journeys, arguing that migrations from the margins of the British Empire and across London form part of a ‘spatial moral order’. Exploring how a distinct pop group emerged at a particular time, it emphasises the importance of family and friendship within the suburban landscape of school, home, a pub, church halls, and a scout hut. A focus on the formation of Wham! contributes to debates about the postcolonial journeys and suburban circumstances that have shaped UK popular music since the end of World War II, and illustrates how psychogeography can contribute to the study of popular music.
A table of n-component handlebody links of genus n+1 up to six crossings
Authors: BELLETTINI GIOVANNI , PAOLINI GIOVANNI , PAOLINI MAURIZIO , WANG YI-SHENG
Published: June 2022

A handlebody link is a union of handlebodies of positive genus embedded in 3-space, which generalises the notion of links in classical knot theory. In this paper, we consider handlebody links with a genus two handlebody and
$n-1$
solid tori,
$n>1$
. Our main result is the classification of such handlebody links with six crossings or less, up to ambient isotopy.
Europe's Final Frontier: Astroculture and Planetary Power since 1945
Authors: Goossen Benjamin W.
Published: June 2022

The main landmass of Europe does not appear in the iconic Blue Marble photograph of earth, taken from space on the final Apollo mission to the moon in December 1972. Europe as a continent remains out of frame, hidden north beyond the curvature of the planet. Viewers instead see swirling clouds, vast expanses of the world ocean and the partially obscured forms of Africa, Antarctica and the Arabian Peninsula. Decentring the Global North was crucial to the charisma of this image, which for half a century has been a symbol of human unity and a staple of appeals to protect the only planet we have ever inhabited. The universality of the photograph contrasted with the frictions of the Cold War and decolonisation that peaked in the 1960s. Blue Marble is nonetheless deeply ambivalent. Its extraterrestrial vantage was possible thanks to the space race between the United States and the Soviet Union. And the implicit message of the photograph – that the benefits of spaceflight and other advanced technologies would be shared with all peoples as a contribution to global economic development – simultaneously invoked legacies of inequality from the epoch of formal imperialism, itself not yet at an end. Regions visible in Blue Marble, in fact, included territory still administered at the time by Britain, France, Norway, Portugal and Spain.1
Interference of morningglories (Ipomoea spp.) with ‘Fascination’ triploid watermelon
Authors: Arana Jeanine , Meyers Stephen L. , Guan Wenjing , Johnson William G.
Published: June 2022

Morningglories (Ipomoea spp.) are among the most troublesome weeds in cucurbits in the United States; however, little is known about Ipomoea spp. interference with horticultural crops. Two additive design field studies were conducted in 2020 at two locations in Indiana to investigate the interference of ivyleaf morningglory (Ipomoea hederacea Jacq.), entireleaf morningglory (Ipomoea hederacea Jacq. var. integriuscula A. Gray.), and pitted morningglory (Ipomoea lacunosa L.) with triploid watermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai]. Immediately after watermelon was transplanted, Ipomoea spp. seedlings were transplanted into the watermelon planting holes at densities of 0 (weed-free control), 3, 6, 12, 18, and 24 plants 27 m−2. Fruit was harvested once a week for 4 wk, and each fruit was classified as marketable (≥4 kg) or non-marketable (<4 kg). At 1 wk after the final harvest, aboveground biomass samples were collected from 1 m2 per plot and oven-dried to obtain watermelon and Ipomoea spp. dry weight. Seed capsules and the number of seeds in 15 capsules were counted from the biomass sample to estimate seed production. Ipomoea spp. densities increasing from 3 to 24 plants 27 m−2 increased marketable watermelon yield loss from 58% to 99%, reduced marketable watermelon fruit number 49% to 98%, reduced individual watermelon fruit weight 17% to 45%, and reduced watermelon aboveground biomass 83% to 94%. Ipomoea spp. seed production ranged from 549 to 7,746 seeds m−2, greatly increasing the weed seedbank. Ipomoea spp. hindered harvest due to their vines wrapping around watermelon fruits. The most likely reason for watermelon yield loss was interference with light and consequently less dry matter being partitioned into fruit development due to less photosynthesis. Yield loss was attributed to fewer fruits and the weight of each fruit.
Adaptive estimation of continuous gait phase based on capacitive sensors
Authors: Xu Dongfang , Zhang Zhitong , Crea Simona , Vitiello Nicola , Wang Qining
Published: June 2022

Continuous gait phase plays an important role in robotic prosthesis control. In this paper, we have conducted the offline adaptive estimation (at different speeds and on different ramps) of continuous gait phase of robotic transtibial prosthesis based on the adaptive oscillators. We have used the capacitive sensing method to record the deformation of the muscles. Two transtibial amputees joined in this study. Based on the strain signals of the prosthetic foot and the capacitive signals of the residual limb, the maximum and minimum of estimation errors are 0.80 rad and 0.054 rad, respectively, and their corresponding ratios in one gait cycle are 1.27% and 0.86%, respectively. This paper proposes an effective method to estimate the continuous gait phase based on the capacitive signals of the residual muscles, which provides a basis for the continuous control of robotic transtibial prosthesis.
A novel microRNA, novel-m009C, regulates methamphetamine rewarding effects
Authors: Zhu Li , Wu Feifei , Yan Zhilan , He Lijun , Wang Shufei , Hu Haohao , Goh Eyleen L. K. , Zhu Yingjie , Guan Fanglin , Chen Teng
Published: June 2022

Methamphetamine (METH) is a widely abused psychostimulant, whose hyper-rewarding property is believed to underlie its addictive effect, but the molecular mechanism regulating this effect remains unclear. We previously reported that decreased expression of a novel microRNA (miRNA), novel-m009C, is implicated in the regulation of METH hyperlocomotion. Here, we found that novel-m009C may be homologous to hsa-miR-604. Its expression is consistently downregulated in the nucleus accumbens (NAc) of mice when exposed to METH and cocaine, whereas significant alterations in novel-m009C expression were not observed in the NAc of mice subjected to other rewarding and psychiatric stimuli, such as sucrose, morphine and MK-801. We further found the substantial reduction in novel-m009C expression may be regulated by both dopamine receptor D1 (D1R) and D2 (D2R). Increasing novel-m009C levels in the NAc attenuated METH-induced conditioned place preference (CPP) and hyperlocomotion, whereas inhibiting novel-m009C expression in the NAc enhanced these effects but did not change the preference of mice for a natural reward, i.e., sucrose. These effects may involve targeting of genes important for the synaptic transmission, such as Grin1 (NMDAR subunit 1). Our findings demonstrate an important role for NAc novel-m009C in regulating METH reward, reveal a novel molecular regulator of the actions of METH on brain reward circuitries and provide a new strategy for treating METH addiction based on the modulation of small non-coding RNAs.
Endosymbiont population genomics sheds light on transmission mode, partner specificity, and stability of the scaly-foot snail holobiont
Authors: Lan Yi , Sun Jin , Chen Chong , Wang Hao , Xiao Yao , Perez Maeva , Yang Yi , Kwan Yick Hang , Sun Yanan , Zhou Yadong , Han Xiqiu , Miyazaki Junichi , Watsuji Tomo-o , Bissessur Dass , Qiu Jian-Wen , Takai Ken , Qian Pei-Yuan
Published: June 2022

The scaly-foot snail (Chrysomallon squamiferum) inhabiting deep-sea hydrothermal vents in the Indian Ocean relies on its sulphur-oxidising gammaproteobacterial endosymbionts for nutrition and energy. In this study, we investigate the specificity, transmission mode, and stability of multiple scaly-foot snail populations dwelling in five vent fields with considerably disparate geological, physical and chemical environmental conditions. Results of population genomics analyses reveal an incongruent phylogeny between the endosymbiont and mitochondrial genomes of the scaly-foot snails in the five vent fields sampled, indicating that the hosts obtain endosymbionts via horizontal transmission in each generation. However, the genetic homogeneity of many symbiont populations implies that vertical transmission cannot be ruled out either. Fluorescence in situ hybridisation of ovarian tissue yields symbiont signals around the oocytes, suggesting that vertical transmission co-occurs with horizontal transmission. Results of in situ environmental measurements and gene expression analyses from in situ fixed samples show that the snail host buffers the differences in environmental conditions to provide the endosymbionts with a stable intracellular micro-environment, where the symbionts serve key metabolic functions and benefit from the host’s cushion. The mixed transmission mode, symbiont specificity at the species level, and stable intracellular environment provided by the host support the evolutionary, ecological, and physiological success of scaly-foot snail holobionts in different vents with unique environmental parameters.
MEX3A promotes the malignant progression of ovarian cancer by regulating intron retention in TIMELESS
Authors: Li Fangfang , Zhao Chen , Diao Yuchao , Wang Zixiang , Peng Jiali , Yang Ning , Qiu Chunping , Kong Beihua , Li Yingwei
Published: June 2022

The latest research shows that RNA-binding proteins (RBPs) could serve as novel potential targets for cancer therapy. We used bioinformatics analysis to screen and identify the key RBPs in ovarian cancer, from which we found that Mex-3 RNA Binding Family Member A (MEX3A) was intimately associated with the clinical prognosis of ovarian cancer. Nevertheless, little is known about its biological roles in ovarian cancer. In this case, we observed that MEX3A was highly overexpressed in fresh-frozen ovarian cancer tissues. MEX3A knockdown suppressed the development and invasion of ovarian cancer cells, while MEX3A overexpression promoted the proliferation and invasion of ovarian cancer cells. Mechanistically, TIMELESS was the critical downstream target gene of MEX3A, as demonstrated through alternative splicing event analysis based on RNA-seq. MEX3A knockdown resulted in retention of intron twenty-three of TIMELESS mRNA and decreased TIMELESS mRNA owing to stimulation of nonsense-mediated RNA decay (NMD). Additionally, we found that TIMELESS overexpression with MEX3A knockdown partially restored the proliferation ability of ovarian cancer cells. The results of this paper demonstrated that the MEX3A/TIMELESS signaling pathway was a key regulator of ovarian cancer, and MEX3A was a novel possible treatment target for ovarian cancer patients.
Itch and autophagy-mediated NF-κB activation contributes to inhibition of cathepsin D-induced sensitizing effect on anticancer drugs
Authors: Seo Seung Un , Woo Seon Min , Min Kyoung-jin , Kwon Taeg Kyu
Published: June 2022

Inhibition of cathepsin D (Cat D) sensitizes cancer cells to anticancer drugs via RNF183-mediated downregulation of Bcl-xL expression. Although NF-κB activation is involved in the upregulation of RNF183 expression, the molecular mechanism of NF-κB activation by Cat D inhibition is unknown. We conducted this study to investigate the molecular mechanism underlying Cat D-mediated NF-κB activation. Interestingly, Cat D inhibition-induced IκB degradation in an autophagy-dependent manner. Knockdown of autophagy-related genes (ATG7 and Beclin1) and lysosome inhibitors (chloroquine and bafilomycin A1) blocked IκB degradation via Cat D inhibition. Itch induced K63-linked ubiquitination of IκB and then modulated the protein stability of IκB by Cat D inhibition. Inhibition of Cat D-mediated Itch activation was modulated by the JNK signaling pathway, and phosphorylated Itch could bind to IκB, resulting in polyubiquitination of IκB. Additionally, inhibition of Cat D increased autophagy flux via activation of the LKB1-AMPK-ULK1 pathway. Therefore, our results suggested that Cat D inhibition activated NF-κB signaling via degradation of autophagy-dependent IκB, which is associated with the upregulation of RNF183, an E3 ligase of Bcl-xL. Cat D inhibition enhances TRAIL-induced apoptosis through Bcl-xL degradation via upregulation of RNF183.
A bipotential organoid model of respiratory epithelium recapitulates high infectivity of SARS-CoV-2 Omicron variant
Authors: Chiu Man Chun , Li Cun , Liu Xiaojuan , Yu Yifei , Huang Jingjing , Wan Zhixin , Xiao Ding , Chu Hin , Cai Jian-Piao , Zhou Biao , Sit Ko-Yung , Au Wing-Kuk , Wong Kenneth Kak-Yuen , Li Gang , Chan Jasper Fuk-Woo , To Kelvin Kai-Wang , Chen Zhiwei , Jiang Shibo , Clevers Hans , Yuen Kwok Yung , Zhou Jie
Published: June 2022

The airways and alveoli of the human respiratory tract are lined by two distinct types of epithelium, which are the primary targets of respiratory viruses. We previously established long-term expanding human lung epithelial organoids from lung tissues and developed a ‘proximal’ differentiation protocol to generate mucociliary airway organoids. However, a respiratory organoid system with bipotential of the airway and alveolar differentiation remains elusive. Here we defined a ‘distal’ differentiation approach to generate alveolar organoids from the same source for the derivation of airway organoids. The alveolar organoids consisting of type I and type II alveolar epithelial cells (AT1 and AT2, respectively) functionally simulate the alveolar epithelium. AT2 cells maintained in lung organoids serve as progenitor cells from which alveolar organoids derive. Moreover, alveolar organoids sustain a productive SARS-CoV-2 infection, albeit a lower replicative fitness was observed compared to that in airway organoids. We further optimized 2-dimensional (2D) airway organoids. Upon differentiation under a slightly acidic pH, the 2D airway organoids exhibit enhanced viral replication, representing an optimal in vitro correlate of respiratory epithelium for modeling the high infectivity of SARS-CoV-2. Notably, the higher infectivity and replicative fitness of the Omicron variant than an ancestral strain were accurately recapitulated in these optimized airway organoids. In conclusion, we have established a bipotential organoid culture system able to reproducibly expand the entire human respiratory epithelium in vitro for modeling respiratory diseases, including COVID-19.
Core and rod structures of a thermophilic cyanobacterial light-harvesting phycobilisome
Authors: Kawakami Keisuke , Hamaguchi Tasuku , Hirose Yuu , Kosumi Daisuke , Miyata Makoto , Kamiya Nobuo , Yonekura Koji
Published: June 2022

Cyanobacteria, glaucophytes, and rhodophytes utilize giant, light-harvesting phycobilisomes (PBSs) for capturing solar energy and conveying it to photosynthetic reaction centers. PBSs are compositionally and structurally diverse, and exceedingly complex, all of which pose a challenge for a comprehensive understanding of their function. To date, three detailed architectures of PBSs by cryo-electron microscopy (cryo-EM) have been described: a hemiellipsoidal type, a block-type from rhodophytes, and a cyanobacterial hemidiscoidal-type. Here, we report cryo-EM structures of a pentacylindrical allophycocyanin core and phycocyanin-containing rod of a thermophilic cyanobacterial hemidiscoidal PBS. The structures define the spatial arrangement of protein subunits and chromophores, crucial for deciphering the energy transfer mechanism. They reveal how the pentacylindrical core is formed, identify key interactions between linker proteins and the bilin chromophores, and indicate pathways for unidirectional energy transfer.
Asynchronous glutamate release is enhanced in low release efficacy synapses and dispersed across the active zone
Authors: Mendonça Philipe R. F. , Tagliatti Erica , Langley Helen , Kotzadimitriou Dimitrios , Zamora-Chimal Criseida G. , Timofeeva Yulia , Volynski Kirill E.
Published: June 2022

The balance between fast synchronous and delayed asynchronous release of neurotransmitters has a major role in defining computational properties of neuronal synapses and regulation of neuronal network activity. However, how it is tuned at the single synapse level remains poorly understood. Here, using the fluorescent glutamate sensor SF-iGluSnFR, we image quantal vesicular release in tens to hundreds of individual synaptic outputs from single pyramidal cells with 4 millisecond temporal and 75 nm spatial resolution. We find that the ratio between synchronous and asynchronous synaptic vesicle exocytosis varies extensively among synapses supplied by the same axon, and that the synchronicity of release is reduced at low release probability synapses. We further demonstrate that asynchronous exocytosis sites are more widely distributed within the release area than synchronous sites. Together, our results reveal a universal relationship between the two major functional properties of synapses – the timing and the overall efficacy of neurotransmitter release.
The architecture and operating mechanism of a cnidarian stinging organelle
Authors: Karabulut Ahmet , McClain Melainia , Rubinstein Boris , Sabin Keith Z. , McKinney Sean A. , Gibson Matthew C.
Published: June 2022

The stinging organelles of jellyfish, sea anemones, and other cnidarians, known as nematocysts, are remarkable cellular weapons used for both predation and defense. Nematocysts consist of a pressurized capsule containing a coiled harpoon-like thread. These structures are in turn built within specialized cells known as nematocytes. When triggered, the capsule explosively discharges, ejecting the coiled thread which punctures the target and rapidly elongates by turning inside out in a process called eversion. Due to the structural complexity of the thread and the extreme speed of discharge, the precise mechanics of nematocyst firing have remained elusive7. Here, using a combination of live and super-resolution imaging, 3D electron microscopy, and genetic perturbations, we define the step-by-step sequence of nematocyst operation in the model sea anemone Nematostella vectensis. This analysis reveals the complex biomechanical transformations underpinning the operating mechanism of nematocysts, one of nature’s most exquisite biological micro-machines. Further, this study will provide insight into the form and function of related cnidarian organelles and serve as a template for the design of bioinspired microdevices.
Functional but not obligatory link between microsaccades and neural modulation by covert spatial attention
Authors: Liu Baiwei , Nobre Anna C. , van Ede Freek
Published: June 2022

Covert spatial attention is associated with spatial modulation of neural activity as well as with directional biases in fixational eye movements known as microsaccades. We studied how these two ‘fingerprints’ of attention are interrelated in humans. We investigated spatial modulation of 8-12 Hz EEG alpha activity and microsaccades when attention is directed internally within the spatial layout of visual working memory. Consistent with a common origin, spatial modulations of alpha activity and microsaccades co-vary: alpha lateralisation is stronger in trials with microsaccades toward versus away from the memorised location of the to-be-attended item and occurs earlier in trials with earlier microsaccades toward this item. Critically, however, trials without attention-driven microsaccades nevertheless show clear spatial modulation of alpha activity – comparable to trials with attention-driven microsaccades. Thus, directional biases in microsaccades correlate with neural signatures of spatial attention, but they are not necessary for neural modulation by spatial attention to be manifest.
Freezing solute atoms in nanograined aluminum alloys via high-density vacancies
Authors: Wu Shenghua , Soreide Hanne S. , Chen Bin , Bian Jianjun , Yang Chong , Li Chunan , Zhang Peng , Cheng Pengming , Zhang Jinyu , Peng Yong , Liu Gang , Li Yanjun , Roven Hans J. , Sun Jun
Published: June 2022

Low-temperature decomposition of supersaturated solid solution into unfavorable intergranular precipitates is a long-standing bottleneck limiting the practical applications of nanograined aluminum alloys that are prepared by severe plastic deformation. Minimizing the vacancy concentration is generally regarded as an effective approach in suppressing the decomposition process. Here we report a counterintuitive strategy to stabilize supersaturated solid solution in nanograined Al-Cu alloys via high-density vacancies in combination with Sc microalloying. By generating a two orders of magnitude higher concentration of vacancies bonded in strong (Cu, Sc, vacancy)-rich atomic complexes, a high thermal stability is achieved in an Al-Cu-Sc alloy that precipitation is nearly suppressed up to ~230 °C. The solute-vacancy complexes also enable the nanograined Al-Cu alloys with higher strength, greater strain hardening capability and ductility. These findings provide perspectives towards the great potentials of solute-vacancy interaction and the development of nanograined alloys with high stability and well-performed mechanical properties.
Possible association of 16p11.2 copy number variation with altered lymphocyte and neutrophil counts
Authors: Giannuzzi Giuliana , Chatron Nicolas , Mannik Katrin , Auwerx Chiara , Pradervand Sylvain , Willemin Gilles , Hoekzema Kendra , Nuttle Xander , Chrast Jacqueline , Sadler Marie C. , Porcu Eleonora ,   16p11.2 Consortium, Männik Katrin , Sanlaville Damien , Schluth-Bolard Caroline , Le Caignec Cédric , Nizon Mathilde , Martin Sandra , Jacquemont Sébastien , Bottani Armand , Gérard Marion , Weber Sacha , Jacquette Aurélia , Vincent-Delorme Catherine , Currò Aurora , Mari Francesca , Renieri Alessandra , Brusco Alfredo , Ferrero Giovanni Battista , Herault Yann , Isidor Bertrand , Gilbert-Dussardier Brigitte , Eichler Evan E. , Kutalik Zoltan , Reymond Alexandre
Published: June 2022

Recurrent copy-number variations (CNVs) at chromosome 16p11.2 are associated with neurodevelopmental diseases, skeletal system abnormalities, anemia, and genitourinary defects. Among the 40 protein-coding genes encompassed within the rearrangement, some have roles in leukocyte biology and immunodeficiency, like SPN and CORO1A. We therefore investigated leukocyte differential counts and disease in 16p11.2 CNV carriers. In our clinically-recruited cohort, we identified three deletion carriers from two families (out of 32 families assessed) with neutropenia and lymphopenia. They had no deleterious single-nucleotide or indel variant in known cytopenia genes, suggesting a possible causative role of the deletion. Noticeably, all three individuals had the lowest copy number of the human-specific BOLA2 duplicon (copy-number range: 3–8). Consistent with the lymphopenia and in contrast with the neutropenia associations, adult deletion carriers from UK biobank (n = 74) showed lower lymphocyte (Padj = 0.04) and increased neutrophil (Padj = 8.31e-05) counts. Mendelian randomization studies pinpointed to reduced CORO1A, KIF22, and BOLA2-SMG1P6 expressions being causative for the lower lymphocyte counts. In conclusion, our data suggest that 16p11.2 deletion, and possibly also the lowest dosage of the BOLA2 duplicon, are associated with low lymphocyte counts. There is a trend between 16p11.2 deletion with lower copy-number of the BOLA2 duplicon and higher susceptibility to moderate neutropenia. Higher numbers of cases are warranted to confirm the association with neutropenia and to resolve the involvement of the deletion coupled with deleterious variants in other genes and/or with the structure and copy number of segments in the CNV breakpoint regions.
High responsivity and flexible deep-UV phototransistor based on Ta-doped β-Ga2O3
Authors: Li Xiao-Xi , Zeng Guang , Li Yu-Chun , Zhang Hao , Ji Zhi-Gang , Yang Ying-Guo , Luo Man , Hu Wei-Da , Zhang David Wei , Lu Hong-Liang
Published: June 2022

Deep-ultraviolet (DUV) phototransistors have shown great potential applications in UV imaging, artificial intelligence, and wearable optoelectronics. Among a large number of wide bandgap semiconductors, the quasi-two-dimensional β-Ga2O3 is considered as an ideal candidate for DUV photodetector applications. Herein, we report a high responsivity (R) and fully flexible Ta-doped β-Ga2O3 DUV phototransistor which exhibits outstanding optoelectrical properties with a high R of 1.32 × 106 A/W, a large detectivity of 5.68 × 1014 Jones, a great photo-to-dark current ratio of 1.10 × 1010%, a high external quantum efficiency of 6.60 × 108%, and an ultra-fast response time of ~3.50 ms. Besides, the flexible Ta-doped β-Ga2O3 device also displays high reliability and mechanical flexibility that can sustain well after over 1 × 104 bending cycles. Moreover, high-contrast imaging of UV light was obtained on the flexible DUV detector arrays, which can be efficiently trained and recognized by an artificial neural network. Our findings offer a perspective to develop wearable optoelectronics and UV imaging based on high-performance flexible β-Ga2O3 DUV phototransistors, providing an inspiration for the future work in artificial intelligence and bionic robot fields.
CORDATA: an open data management web application to select corrosion inhibitors
Authors: Galvão Tiago L. P. , Ferreira Inês , Kuznetsova Alena , Novell-Leruth Gerard , Song Ci , Feiler Christian , Lamaka Sviatlana V. , Rocha Claúdia , Maia Frederico , Zheludkevich Mikhail L. , Gomes José R. B. , Tedim João
Published: June 2022

The large amount of corrosion inhibition efficiencies in literature, calls for a more efficient way to organize, access and compare this information. The CORDATA open data management application (https://datacor.shinyapps.io/cordata/) can help select appropriate corrosion inhibitors for application specific challenges.