bluuebunny/crossref_metadata_2025 huggingface dataset:
Asymmetric and uncertain interactions within mutualisms
Authors: Wang Rui-Wu , Shi An-Na , Zhang Xiao-Wei , Liu Min , Jandér K Charlotte , Dunn Derek W
Published: December 2023

Although understanding mutualism stability has advanced over the last few decades, two fundamental problems still remain in explaining how mutualisms maintain stable. (i) How does resolve conflict between mutualists over resources? (ii) In the presence of less cooperative and/or uncooperative symbionts, how does prevent symbiont populations from becoming dominated by uncooperative individuals? Many past explanations of mutualism stability have assumed that interactions between mutualists are symmetrical. However, in most mutualisms, interactions between hosts and symbionts show varying degrees of asymmetry at different levels. Here, we review three major types of asymmetric interactions within obligate mutualisms: (i) asymmetric payoffs, which is also defined as individual power differences, (ii) asymmetric potential rates of evolutionary change, and (iii) asymmetric information states between hosts and symbionts. We suggest that these asymmetries between mutualists help explain why cooperation and conflict are inherent in the evolution of mutualisms, and why both hosts and symbionts present diversified phenotypes while cooperation predominates.
Leaf stoichiometry of common species along altitude gradients in the Qilian Mountains, China
Authors: Hong Shuyan , Chen Jie , Biswas Asim , Cao Jianjun , Dong Xiaogang
Published: December 2023

Investigating the variations in leaf stoichiometry among plant common species at different altitudes, along with the factors that influence these variations and the adaptative strategies employed, is of significant importance for understanding biogeochemical cycles amidst global environmental changes. In this research, we measured soil organic carbon and nutrient concentrations, as well as leaf stoichiometry for plant common species at five altitudes (2400–3200 m with an interval of 200 m) within the Qilian Mountains of Northwest China. This study aims to enhance our understanding of how plant common species in mountainous regions exhibit adaptable responses to altitude variations and how potential environmental changes in the future may influence their leaf functions. Results showed that the leaf C:N:P stoichiometry of plant common species varied with increasing altitude. Across altitudes, mean annual temperature (MAT), soil total phosphorus, mean annual precipitation (MAP), soil water content, and soil nitrate nitrogen were the main factors influencing leaf element concentrations of plant common species. However, leaf stoichiometric ratios were mainly determined by MAT, MAP, and soil total nitrogen. The effects of MAT and MAP on both leaf element concentrations and leaf stoichiometric ratios of plant common species were found to be significant. Plant growth in the study area was mainly limited by P. The results not only highlight the adaptive strategies employed by plants, but also contribute to understanding of leaf stoichiometry, and establishing connections between individual plant species and broader plant community composed of these common species.
Online Intervention Targeting Postnatal Depression and Anxiety in Chilean First-Time Mothers: Feasibility Trial
Authors: Coo Soledad , García María Ignacia , Pérez J Carola , Aldoney Daniela , Olhaberry Marcia , Fernández Olga , Alamo Nicolle , Franco Pamela , Pérez Francisca , Fernández Sofía , Fisher Jane , Rowe Heather
Published: December 2023

Objective

Internet-based interventions may positively impact maternal symptoms of postnatal depression and anxiety. This study assessed the feasibility, acceptability, perceived usefulness, and preliminary effectiveness of an m-Health version of “What Were We Thinking?” (m-WWWT).

Methods

A mixed-methods with a 2-arm randomized parallel design was used. From a total of 477 women, 157 met the inclusion criteria. 128 first-time mothers of full-term infants, aged 4–10 weeks, who received health care at primary public health centers in Chile, were randomly assigned to the experimental (EG, n = 65) or control (CG, n = 63) groups; data of 104 of them (53 and 51, respectively) was analyzed. We used percentages and rates to measure feasibility outcomes and mixed analysis of variance (ANOVA) and latent class analyses (LCA) to assess preliminary effectiveness. Participants completed questionnaires on mental health, social support, and maternal self-efficacy upon recruitment and 3 months after completing the intervention. For the qualitative component, 12 women from the EG were interviewed.

Results

Quantitative results show good feasibility outcomes, such as high recruitment (82%), low attrition (EG = 12% and CG = 17%), and high follow-up (EG = 97% and CG = 91%) rates. Qualitative results indicate high acceptability and perceived usefulness of m-WWWT. Mixed ANOVA did not show significant differences between the groups (all p >.05). However, multinomial regression analysis in LCA showed that women with low baseline symptoms of depression and anxiety benefit from the intervention (B = 0.43, 95% confidence interval 1.09–2.16).

Conclusion

m-WWWT is feasible to be implemented in Chile; future studies are needed to assess the intervention’s effectiveness.
International law as shibboleth: the continued appeal of heroic narratives in support of military intervention
Authors: Mitchell Lynsey
Published: December 2023

Political debates on the use of force draw on Manichean narratives which are legitimated by legalistic language. Overreliance on such narratives devalues international law as a safeguard against the illegal use of force, silences criticism that militarism is not the solution to international crises, and blurs legal and non-legal justifications for intervention.
Review of treatment options for a multidrug-resistant fungus: Candida auris
Authors: Wang Siqi , Pan Jiangwei , Gu Liting , Wang Wei , Wei Bin , Zhang Huawei , Chen Jianwei , Wang Hong
Published: December 2023

Candida auris is a widely distributed, highly lethal, multidrug-resistant fungal pathogen. It was first identified in 2009 when it was isolated from fluid drained from the external ear canal of a patient in Japan. Since then, it has caused infectious outbreaks in over 45 countries, with mortality rates approaching 60%. Drug resistance is common in this species, with a large proportion of isolates displaying fluconazole resistance and nearly half are resistant to two or more antifungal drugs. In this review, we describe the drug resistance mechanism of C. auris and potential small-molecule drugs for treating C. auris infection. Among these antifungal agents, rezafungin was approved by the US Food and Drug Administration (FDA) for the treatment of candidemia and invasive candidiasis on March 22, 2023. Ibrexafungerp and fosmanogepix have entered phase III clinical trials.
α-enhanced astrochemistry: the carbon cycle in extreme galactic conditions
Authors: Bisbas Thomas G , Zhang Zhi-Yu , Gjergo Eda , Zhao Ying-He , Luo Gan , Quan Donghui , Jiang Xue-Jian , Sun Yichen , Topkaras Theodoros , Li Di , Guo Ziyi
Published: December 2023

Astrochemistry has been widely developed as a power tool to probe the physical properties of the interstellar medium (ISM) in various conditions of the Milky Way (MW) Galaxy, and in near and distant galaxies. Most current studies conventionally apply linear scaling to all elemental abundances based on the gas-phase metallicity. However, these elements, including carbon and oxygen, are enriched differentially by stellar nucleosynthesis and the overall galactic chemical evolution, evident from α-enhancement in multiple galactic observations such as starbursts, high-redshift star-forming galaxies, and low-metallicity dwarfs. We perform astrochemical modelling to simulate the impact of an α-enhanced ISM gas cloud on the abundances of the three phases of carbon (C+, C, CO) dubbed as ‘the carbon cycle’. The ISM environmental parameters considered include two cosmic-ray ionization rates (ζCR = 10−17 and $10^{-15}\, {\rm s}^{-1}$), two isotropic FUV radiation field strengths (χ/χ0 = 1 and 102), and (sub-)linear dust-to-gas relations against metallicity, mimicking the ISM conditions of different galaxy types. In galaxies with [C/O] < 0, CO, C, and C+, all decrease in both abundances and emission, though with differential biases. The low-J CO emission is found to be the most stable tracer for the molecular gas, while C and C+ trace H2 gas only under limited conditions, in line with recent discoveries of [C i]-dark galaxies. We call for caution when using [C ii] $158\, \mu$m and [C i](1–0) as alternative H2-gas tracers for both diffuse and dense gas with non-zero [C/O] ratios.
TOI-4641b: an aligned warm Jupiter orbiting a bright (V=7.5) rapidly rotating F-star
Authors: Bieryla Allyson , Zhou George , García-Mejía Juliana , Fairnington Tyler R , Latham David W , Carter Brad , Dong Jiayin , Huang Chelsea X , Murphy Simon J , Shporer Avi , Collins Karen A , Quinn Samuel N , Everett Mark E , Buchhave Lars A , Tronsgaard René , Charbonneau David , Johnson Marshall C , Esquerdo Gilbert A , Calkins Michael , Berlind Perry , Jenkins Jon M , Ricker George R , Seager Sara , Winn Joshua N , Barclay Thomas , Mireles Ismael , Paegert Martin , Twicken Joseph D
Published: December 2023

We report the discovery of TOI-4641b, a warm Jupiter transiting a rapidly rotating F-type star with a stellar effective temperature of 6560 K. The planet has a radius of 0.73 RJup, a mass smaller than 3.87 MJup(3σ), and a period of 22.09 d. It is orbiting a bright star (V=7.5 mag) on a circular orbit with a radius and mass of 1.73 R⊙ and 1.41 M⊙. Follow-up ground-based photometry was obtained using the Tierras Observatory. Two transits were also observed with the Tillinghast Reflector Echelle Spectrograph, revealing the star to have a low projected spin-orbit angle (λ=$1.41^{+0.76}_{-0.76}$°). Such obliquity measurements for stars with warm Jupiters are relatively few, and may shed light on the formation of warm Jupiters. Among the known planets orbiting hot and rapidly rotating stars, TOI-4641b is one of the longest period planets to be thoroughly characterized. Unlike hot Jupiters around hot stars which are more often misaligned, the warm Jupiter TOI-4641b is found in a well-aligned orbit. Future exploration of this parameter space can add one more dimension to the star–planet orbital obliquity distribution that has been well sampled for hot Jupiters.
Keeping a track on leptomeningeal disease in non–small cell lung cancer: A single-institution experience with CNSideTM
Authors: Puri Sonam , Malani Rachna , Chalmers Anna , Kerrigan Kathleen , Patel Shiven B , Monynahan Kelly , Cannon Laura , Blouw Barbara , Akerley Wallace
Published: December 2023

Background

Leptomeningeal disease (LMD) is a devastating complication for patients with advanced cancer. Diagnosis and monitoring the response to therapy remains challenging due to limited sensitivity and specificity of standard-of-care (SOC) diagnostic modalities, including cerebrospinal fluid (CSF) cytology, MRI, and clinical evaluation. These hindrances contribute to the poor survival of LMD patients. CNSide is a CLIA-validated test that detects and characterizes CSF-derived tumor cells and cell-free (cf) DNA. We performed a retrospective analysis on the utility of CNSide to analyze CSF obtained from advanced non–small cell lung cancer (aNSCLC) patients with suspected LMD treated at the Huntsman Cancer Institute in Salt Lake City, UT.

Methods

CNSide was used to evaluate CSF from 15 patients with aNSCLC. CSF tumor cell quantification was performed throughout treatment for 5 patients. CSF tumor cells and cfDNA were characterized for actionable mutations.

Results

In LMD-positive patients, CNSide detected CSF tumor cells in 88% (22/25) samples versus 40% (10/25) for cytology (matched samples). CSF tumor cell numbers tracked response to therapy in 5 patients where CNSide was used to quantify tumor cells throughout treatment. In 75% (9/12) of the patients, genetic alterations were detected in CSF, with the majority representing gene mutations and amplifications with therapeutic potential. The median survival for LMD patients was 16.1 m (5.2-NR).

Conclusions

We show that CNSide can supplement the management of LMD in conjunction with SOC methods for the diagnosis, monitoring response to therapy, and identifying actionable mutations unique to the CSF in patients with LMD.
How Should a Vape Shop-Based Smoking Cessation Intervention Be Delivered? A Qualitative Study
Authors: Langley Tessa , Young Emily , Hunter Abby , Bains Manpreet
Published: December 2023

Introduction

Encouraging smokers to quit smoking tobacco using e-cigarettes could substantially reduce smoking-related diseases. Vape shops (VS) therefore have the potential to play an important role in supporting smoking cessation. The aim of this study was to explore how to deliver a vape shop-based smoking cessation intervention in the United Kingdom.

Methods

Semistructured telephone interviews were undertaken with four stakeholder groups: 20 stop smoking service (SSS) providers, seven tobacco control leads, seven smokers/vapers, and five vape shop staff. Interviews were analyzed thematically.

Results

Stakeholder groups were positive about the idea of delivering a vape shop-based intervention. Themes that were identified were the characteristics of the intervention (duration and timing; delivery; style and content; and product provisions); barriers to the intervention (challenges for new vapers; false information; tobacco company involvement; and conflicts of interest); facilitators to the intervention (positive views on vaping; cost-effectiveness; popularity; and accessibility); and considerations for the intervention (data protection and privacy; aesthetics; and regulation and management). The results suggest that the intervention should be delivered by vape shop workers with mandatory training with the support of SSS. Most stakeholders agreed quitting vaping was not a priority, but that information on how to reduce nicotine use should be given. Concerns around privacy, General Data Protection Regulation, misinformation about vaping, and tobacco company involvement would need to be addressed.

Conclusions

Stakeholders agree that VS should offer stop smoking interventions and hold similar opinions on how this should be delivered.

Implications

This study suggests that smokers, vapers, and other key stakeholders are positive about the idea of a stop-smoking vape shop-based intervention and that they hold similar opinions on how this should be delivered. Most participants felt that this should be primarily delivered by trained vape shop staff and run with support from SSS. Participants agreed that a stop-smoking vape shop-based intervention should be flexible in terms of the type, duration, and frequency of support provided, and that the intervention should comprise both technical guidance on using a vape and behavioral support to prevent a return to smoking.
Everything but the Kitchen Sink: An Analysis of Bacterial and Chemical Contaminants Found in Syringe Residue From People Who Inject Drugs
Authors: Wildenthal John A , Schwartz Drew J , Nolan Nathanial S , Zhao Lingxia , Robinson John I , Jones Erin , Jawa Raagini , Henderson Jeffrey P , Marks Laura R
Published: December 2023

Background

People who inject drugs (PWID) are at high risk of severe wounds, invasive infections, and overdoses. To date, there are few data on the bacterial and chemical contaminants PWID are exposed to when using illicitly manufactured fentanyls and stimulants.

Methods

Previously used injection drug use equipment was recovered in St Louis, Missouri, by harm reduction organizations over a 12-month period. Syringe residue was analyzed for bacterial contaminants by routine culturing followed by whole genome sequencing of single bacterial isolates. Chemical adulterants in syringe residue were identified by liquid chromatography–mass spectrometry.

Results

Bacteria were cultured from 58.75% of 160 syringes analyzed. Polymicrobial growth was common and was observed in 23.75% of samples. Bacillus cereus was the most common pathogen present and was observed in 20.6% of syringe residues, followed closely by Staphylococcus aureus at 18.8%. One hundred syringes underwent mass spectrometry, which demonstrated that chemical adulterants were common and included caffeine, diphenhydramine, lidocaine, quinine, and xylazine.

Conclusions

Analysis of syringe residue from discarded drug use equipment demonstrates both chemical and biological contaminants, including medically important pathogens and adulterants.
Efficacy of Single-Agent Chemotherapy in Endocrine Therapy-Refractory Metastatic Invasive Lobular Carcinoma
Authors: Mouabbi Jason A , Qaio Wei , Shen Yu , Raghavendra Akshara Singareeka , Tripathy Debasish , Layman Rachel M
Published: December 2023

Background

Hormone receptor (HR)-positive, HER2-negative metastatic invasive lobular breast cancer (mILC) is distinct from invasive ductal cancer (IDC) in clinicopathologic and molecular characteristics, impacting its response to systemic therapy. While endocrine therapy (ET) combined with targeted therapies has shown efficacy in ET-sensitive mILC, data on chemotherapy in ET-refractory mILC remain limited. We investigated the efficacy of single-agent capecitabine (CAP) versus taxanes (TAX) in ET-refractory HR+ HER2-negative patients with mILC.

Materials and Methods

Using data from the MD Anderson prospectively collected breast cancer database, we identified patients with HR+ HER2-negative mILC who received prior ET and first-time chemotherapy in the metastatic setting. We compared outcomes between 173 CAP-treated and 96 TAX-treated patients.

Results

CAP-treated patients had significantly better median progression-free survival (PFS) than TAX-treated patients (8.8 vs 5.0 months, HR 0.63, P < .001). Overall survival (OS) did not differ significantly between the groups (42.7 vs 36.6 months for CAP vs TAX, respectively, HR 0.84, P = .241). Multivariate analyses for PFS and OS revealed better outcomes in subjects with fewer metastatic sites and those exposed to more lines of ET. Additionally, Black patients showed worse OS outcomes compared to White patients (HR 2.46; P = .001).

Conclusion

In ET-refractory HR+ HER2-negative mILC, single-agent CAP demonstrated superior PFS compared to TAX. Our findings highlight the potential benefit of CAP in this patient subset, warranting further investigation through prospective trials.
Methotrexate Scarcity Among Children’s Oncology Group Institutions: Results of a Multinational Survey
Authors: Menig Sarah , Militano Olga , Ostrenga Andrew , Bernhardt M Brooke , Lee Brittany , Unguru Yoram
Published: December 2023

Shortages of curative chemotherapy agents for children and adults with cancer are ubiquitous. These shortages directly result in compromised outcomes, increased medication errors, heightened cost to health systems, and patient deaths. Methotrexate is a staple of many curative childhood cancer regimens and is frequently in scarcity. No national guidance to manage methotrexate and other chemotherapy shortages exists. To assess the effect of the current methotrexate shortage, a multinational survey of Children’s Oncology Group (COG) member institutions was conducted. Wide variation in the scope of methotrexate shortage in the US was demonstrated; some centers experienced significant scarcity while others experienced no shortage. Methotrexate mitigation strategies differed by COG site, resulting in potential to exacerbate differential access to life-saving medication and inequities in care. Preventing chemotherapy shortages remains a challenge. In the interim, standard guidance to assist clinicians to equitably and fairly cope with methotrexate and related drug shortages is needed.
Irruptive fall migrations are linked to elevated breeding abundance but are not associated with body condition or stopover duration in Northern Saw-whet Owls
Authors: Craik Shawn R , Doucet Amélie , Manuel Mathieu , Roy Chloé , Brown Taylor M , Knighton Emilie J , Shutler Dave , Lauff Randy F , Ethier Danielle , Kouwenberg Amy-Lee , Taylor Philip D
Published: December 2023

Bird species that undertake irruptive migrations are good candidates for assessing density-dependent effects on stopover ecology because the number of birds using stopover sites varies considerably from year to year. Using morphometric data from a 9-year banding program and radiotelemetry (n = 25 females), we found that increases in the annual density of Northern Saw-whet Owls (NSWO; Aegolius acadicus) encountered at a fall stopover site in Nova Scotia, Canada, were not linked to female body condition or minimum stopover duration. Rather, most NSWO spent no more than 1 or 2 full days at the stopover site following radio-tagging and during their return visits to the site. Body condition indices were highest for NSWO captured near the end of the migration monitoring season, possibly reflecting birds that had recently established wintering ranges and were elevating energy stores. We used breeding abundance indices derived from Birds Canada’s Atlantic Nocturnal Owl Survey to help test the hypothesis that irruptive fall migrations in NSWO were driven by elevated breeding productivity and dispersal of immature birds (breeding success hypothesis). Indeed, irruptive fall migrations were characterized by elevated densities of immatures, but not adults, and mean breeding abundance indices for the Maritime provinces during years with irruptive migrations were higher than those for non-irruptive years. We hypothesize that prey abundance during years with irruptive migrations was sufficient to enable high breeding densities and minimize effects of elevated fall densities of NSWO on rates of mass gain and stopover duration.
Experimental warming of embryos accelerates metabolism of postnatal songbirds, influences growth, but has no effects on parental feeding and brooding rates
Authors: Ton Riccardo , Martin Thomas E
Published: December 2023

Here we investigate possible carryover effects of experimentally increased incubation temperatures for postnatal growth, metabolism, and parental care within and among 6 north temperate and one tropical songbird species. Increased temperatures during embryonic development consistently caused higher postnatal resting metabolism compared to control nests, among but not within the 7 species studied. The effects of the experiment on growth were species specific and depended on the morphometric considered. Size before the fledge date was positively correlated with feeding rate, and metabolism was lower in larger broods. Our experiment did not elicit changes in parental food delivery rates or brooding effort during the postnatal stage, and higher brooding effort was associated with nestlings of smaller mass and faster metabolism independently from treatment. Consequently, parental care seemed unlikely to be the cause of the differences in growth rates between treatments. Instead, physiological mechanisms triggered by our heating treatment appear to be responsible for the observed variation in growth. These intrinsic changes unmatched by adjustments in parental effort may contribute to longer-term consequences for individual quality and survival that deserve further attention.
Long way up: rethink diseases in light of phase separation and phase transition
Authors: Ding Mingrui , Xu Weifan , Pei Gaofeng , Li Pilong
Published: December 2023

Biomolecular condensation, driven by multivalency, serves as a fundamental mechanism within cells, facilitating the formation of distinct compartments, including membraneless organelles that play essential roles in various cellular processes. Perturbations in the delicate equilibrium of condensation, whether resulting in gain or loss of phase separation, have robustly been associated with cellular dysfunction and physiological disorders. As ongoing research endeavors wholeheartedly embrace this newly acknowledged principle, a transformative shift is occurring in our comprehension of disease. Consequently, significant strides have been made in unraveling the profound relevance and potential causal connections between abnormal phase separation and various diseases. This comprehensive review presents compelling recent evidence that highlight the intricate associations between aberrant phase separation and neurodegenerative diseases, cancers, and infectious diseases. Additionally, we provide a succinct summary of current efforts and propose innovative solutions for the development of potential therapeutics to combat the pathological consequences attributed to aberrant phase separation.
The evolving spectrum of human African trypanosomiasis
Authors: Kennedy P G E
Published: December 2023

Human African trypanosomiasis (HAT), or sleeping sickness, continues to be a major threat to human health in 36 countries throughout sub-Saharan Africa with up to 60 million people at risk. Over the last decade, there have been several advances in this area, some of which are discussed in this overview. Due to the concerted efforts of several bodies, including better identification and treatment of cases and improved tsetse fly vector control, the number of cases of HAT has declined dramatically. The clinical heterogeneity of HAT has also been increasingly recognized, and the disease, while usually fatal if untreated or inadequately treated, does not always have a uniformly fatal outcome. Improved methods of HAT diagnosis have now been developed including rapid diagnostic tests. Novel drug treatment of HAT has also been developed, notably nifurtimox–eflornithine combination therapy (NECT) for late-stage Trypanosoma brucei gambiense, oral fexinidazole for early and the early component of the late-stage of T.b. gambiense, and the new oral compounds of the oxaborole group, which have shown considerable promise in field trials. Advances in HAT neuropathogenesis have been steady, though largely incremental, with a particular focus on the role of the blood–brain barrier in parasite entry into the central nervous system and the relevant importance of both innate and adaptive immunity. While the World Health Organization goal of elimination of HAT as a public health problem by 2020 has probably been achieved, it remains to be seen whether the second more ambitious goal of interruption of transmission of HAT by 2030 will be attained.
First contact physiotherapists: are they able to reduce the burden on rheumatology services? A critical review of the evidence base
Authors: Golding Sarah R , Jackson Jo
Published: December 2023

First contact practitioners have emerged over recent years in response to growing pressures within the National Health Service (NHS) and are now central to primary care musculoskeletal (MSK) services. Within the MSK field, these allied health professionals can be from a range of disciplines, including physiotherapy, podiatry and osteopathy. Early referral to rheumatology is key to successful long-term management of many inflammatory MSK conditions, but presents challenges to overburdened services. Evidence supporting the recognition and referral of patients with rheumatological disorders by First Contact Practitioners is lacking; however, physiotherapists have been shown successfully to substitute the role of a doctor within the MSK field. This review investigates the value of First Contact Physiotherapists (FCPs) within primary care and their role in early recognition and referral of rheumatological MSK disorders in line with national guidance. FCPs best placed to fulfil the role of MSK champions, positively impacting the whole MSK pathway, with the potential to reduce the burden on rheumatology services. Planned rapid upscaling of FCPs over the next few years will support sustainability of MSK NHS services.
Self-Disorder in Schizophrenia: A Revised View (2. Theoretical Revision—Hyperreflexivity)
Authors: Sass Louis , Feyaerts Jasper
Published: December 2023

A growing body of research supports the role of self-disorders as core phenotypic features of schizophrenia-spectrum conditions. Self-disorders comprise various alterations of conscious experience whose theoretical understanding continues to present a challenge. This is the second of two articles that aim to clarify the nature of self-disorders in schizophrenia by considering the currently most influential, phenomenological model of schizophrenia: the basic-self-disturbance or ipseity-disorder model (IDM). The previous paper (article 1) presented a state-of-the-art overview of this model and critically assessed its descriptive adequacy with respect to the clinical heterogeneity and variability of the alterations in self- and world-awareness characteristic of schizophrenia. This paper (article 2) proposes a theoretical revision by considering how hyperreflexivity might form the crucial common thread or generating factor that unifies the heterogeneous, and sometimes even contradictory features of schizophrenic self-disorders. We outline implications of our revised model (IDMrevised) for explanatory research, therapeutic practice, and our general understanding of the abnormalities in question.
Sleep deprivation alters pubertal timing in humans and rats: the role of the gut microbiome
Authors: Gunawan Shirley Priscilla , Huang Shih-Yi , Wang Chun-Chi , Huynh Linh Ba Phuong , Nguyen Nam Nhat , Hsu Shih-Yuan , Chen Yang-Ching
Published: December 2023

Study Objectives

Evidence implied that sleeping duration is associated with the timing of puberty and that sleep deprivation triggers early pubertal onset in adolescents. Sleep deprivation can affect metabolic changes and gut microbiota composition. This study investigated the effects of sleep deprivation on pubertal onset and gut microbiota composition in animal models and a human cohort.

Methods

This study comprised 459 boys and 959 girls from the Taiwan Pubertal Longitudinal Study. Sleep duration was evaluated using the self-report Pittsburgh Sleep Quality Index questionnaire. Early sexual maturation was defined by pediatric endocrinologist assessments. Mediation analyses were done to examine the association between sleep parameters, obesity, and early sexual maturation. Besides, Sprague Dawley juvenile rats were exposed to 4 weeks of chronic sleep deprivation. Vaginal opening (VO) and preputial separation (PS) were observed every morning to determine pubertal onset in female and male rats.

Results

The sleep-deprived juvenile rats in the sleep-deprived-female (SDF) and sleep-deprived-male (SDM) groups experienced delayed VO (mean VO days: 33 days in control; 35 days in SDF; p-value < 0.05) and PS (mean PS days: 42 days in control; 45 days in SDM; p-value < 0.05), respectively. Relative to their non-sleep-deprived counterparts, the sleep-deprived juvenile rats exhibited lower body weight and body fat percentage. Significant differences in relative bacterial abundance at genus levels and decreased fecal short-chain-fatty-acid levels were identified in both the SDF and SDM groups. In the human cohort, insufficient sleep increased the risk of early sexual maturation, particularly in girls (OR, 1.44; 95% CI: 1.09 to 1.89; p-value < 0.01). Insufficient sleep also indirectly affected early sexual maturation in girls, with obesity serving as the mediator.

Conclusions

Overall, sleep deprivation altered the timing of puberty in both animal and human models but in different directions. In the rat model, sleep deprivation delayed the pubertal onset in juvenile rats through gut dysbiosis and metabolic changes, leading to a low body weight and body fat percentage. In the human model, sleep deprivation led to fat accumulation, causing obesity in girls, which increased the risk of early puberty.
Digital Platforms and the Maintenance of the Urban Order
Authors: Lara-Millán Armando , Guzman-Garcia Melissa
Published: December 2023

Neighborhood digital platforms – such as Nextdoor, Citizen, Neighbors, anti-crime Facebook groups, Ring surveillance technology, and 311 see-click-fix applications – are recent entrants into urban life. Existing accounts suggest they help build intra-community relationships, but that they also amplify paranoia, racism, and carceral impulses of American homeowners. We ask: how is the new technology increasing solidarity and exclusionary impulses, and what role does it play in the changing American urban landscape? Using offline and online ethnography of one community’s year-long contestation over public space, we find that three effects of the platforms help explain the maintenance of urban order in this case. First, the platforms push residents to see disparate instances of urban disorder as a linked manifestation of organized crime. Second, the platforms help to turn fleeting and uncorroborated accounts into durable events that foster community efficacy. Third, by increasing perceptions of urban disorder and greater community efficacy, the platforms facilitate the accrual of offline material resources. We suggest that in highly contested areas of American cities – areas where wealthy residents vie with a largely Brown and Black working-class for use of space – neighborhood digital platforms help to funnel services that support property value into smaller sections of otherwise disinvested neighborhoods.
Assessing the market viability of a packaged intensive health behavior and lifestyle treatment
Authors: Persaud Alicia , Smith Natalie Riva , Lindros Jeanne , Salmon Jeremiah , Ventura Giustina , Perkins Meghan , Taveras Elsie M , Fiechtner Lauren , Simione Meg
Published: December 2023

In the USA, more than 14 million children are impacted by obesity. Despite intensive health behavior and lifestyle treatments being found effective, gaps exist in moving these interventions into widespread use. Focusing on market viability could improve the dissemination and sustainment of interventions. The purpose of this paper is to outline the process and results of our market viability assessment for the Healthy Weight Clinic (HWC), a Centers for Disease Control and Prevention-recognized Family Health Weight Program. We conducted a market viability assessment using the Speeding Research-test INTerventions (SPRINT) program to gain insights into the commercialization and marketplace for the HWC. Through the process of customer discovery, we interviewed 50 stakeholders to test our hypotheses pertaining to our business model. Key takeaways were the need for packaged interventions that offer support and training for providers, and interventions that are multidisciplinary and located within the medical home. We also learned that (i) the intervention goals must align with the healthcare organization’s performance metrics; (ii) services need to be reimbursable; and (iii) the importance of understanding different customer segments (i.e. program users vs. organization decision-makers) and their unique needs. The market viability assessment is a critical step to transforming the HWC into a viable commercial product. The process we have outlined is replicable by others and by encouraging other teams to design for dissemination we can increase the number of evidence-based, packaged IHBLTs available to children with obesity.
Bypassing the brain barriers: upregulation of serum miR-495 and miR-543-3p reflects thyroid-mediated developmental neurotoxicity in the rat
Authors: O’Shaughnessy Katherine L , Sasser Aubrey L , Bell Kiersten S , Riutta Cal , Ford Jermaine L , Grindstaff Rachel D , Gilbert Mary E
Published: December 2023

Evaluating the neurodevelopmental effects of thyroid-disrupting chemicals is challenging. Although some standardized developmental and reproductive toxicity studies recommend serum thyroxine (T4) measures in developing rats, extrapolating between a serum T4 reduction and neurodevelopmental outcomes is not straightforward. Previously, we showed that the blood-brain and blood-cerebrospinal fluid barriers may be affected by developmental hypothyroidism in newborn rats. Here, we hypothesized that if the brain barriers were functionally disturbed by abnormal thyroid action, then small molecules may escape from the brain tissue and into general circulation. These small molecules could then be identified in blood samples, serving as a direct readout of thyroid-mediated developmental neurotoxicity. To address these hypotheses, pregnant rats were exposed to propylthiouracil (PTU, 0 or 3 ppm) to induce thyroid hormone insufficiency, and dams were permitted to give birth. PTU significantly reduced serum T4 in postnatal offspring. Consistent with our hypothesis, we show that tight junctions of the brain barriers were abnormal in PTU-exposed pups, and the blood-brain barrier exhibited increased permeability. Next, we performed serum microRNA Sequencing (miRNA-Seq) to identify noncoding RNAs that may reflect these neurodevelopmental disturbances. Of the differentially expressed miRNAs identified, 7 were upregulated in PTU-exposed pups. Validation by qRT-PCR shows that miR-495 and miR-543-3p were similarly upregulated in males and females. Interestingly, these miRNAs have been linked to cell junction dysfunction in other models, paralleling the identified abnormalities in the rat brain. Taken together, these data show that miR-495 and miR-543-3p may be novel in vivo biomarkers of thyroid-mediated developmental neurotoxicity.
Responses of stomatal density and carbon isotope composition of sugar maple and yellow birch foliage to N, P and CaSiO3 fertilization
Authors: Zukswert Jenna M , Vadeboncoeur Matthew A , Yanai Ruth D
Published: December 2023

Stomatal density, stomatal length and carbon isotope composition can all provide insights into environmental controls on photosynthesis and transpiration. Stomatal measurements can be time-consuming; it is therefore wise to consider efficient sampling schemes. Knowing the variance partitioning at different measurement levels (i.e., among stands, plots, trees, leaves and within leaves) can aid in making informed decisions around where to focus sampling effort. In this study, we explored the effects of nitrogen (N), phosphorus (P) and calcium silicate (CaSiO3) addition on stomatal density, length and carbon isotope composition (δ13C) of sugar maple (Acer saccharum Marsh.) and yellow birch (Betula alleghaniensis Britton). We observed a positive but small (8%) increase in stomatal density with P addition and an increase in δ13C with N and CaSiO3 addition in sugar maple, but we did not observe effects of nutrient addition on these characteristics in yellow birch. Variability was highest within leaves and among trees for stomatal density and highest among stomata for stomatal length. To reduce variability and increase chances of detecting treatment differences in stomatal density and length, future protocols should consider pretreatment and repeated measurements of trees over time or measure more trees per plot, increase the number of leaf impressions or standardize their locations, measure more stomata per image and ensure consistent light availability.
Species delimitation and phylogenetic analyses of a New Guinean frog genus (Microhylidae: Hylophorbus) reveal many undescribed species and a complex diversification history driven by late Miocene events
Authors: Ferreira Flavien , Kraus Fred , Richards Stephen , Oliver Paul , Günther Rainer , Trilaksono Wahyu , Arida Evy Ayu , Hamidy Amir , Riyanto Awal , Tjaturadi Burhan , Thébaud Christophe , Gaucher Philippe , Fouquet Antoine
Published: December 2023

New Guinea is the largest tropical island in the world and hosts immense endemic biodiversity. However, our understanding of how the gradual emergence of the terrestrial ecosystems of the island over the last 40 Myr has generated this biological richness is hampered by poorly documented species diversity and distributions. Here, we address both these issues through an integrative taxonomy and biogeographical approach using Hylophorbus, a New Guinea-endemic genus of frogs with 12 recognized species. We delimited candidate species by integrating mitochondrial DNA, nuclear DNA, and bioacoustics, then investigated their evolutionary history. Our results suggest that the current taxonomy of the genus misses true species diversity by ≥3.5-fold. Nevertheless, most candidate species (27) remain unconfirmed because of missing data, whereas five were identified unambiguously as undescribed (we describe three of these formally). Time-calibrated phylogenetic analyses suggest that Hylophorbus diversification began ~9 Mya in the northern or eastern portion of New Guinea. It would appear that lineages dispersed to new terrestrial habitats in the west, notably uplifted by the central range orogeny, until eventually reaching the Bird’s Head during the Mio-Pliocene (7–5 Mya). Conversely, a past barrier appears to have prevented north–south dispersal. These data suggest that new habitat availability has primarily driven the diversification of Hylophorbus.
Geometric morphometry enables species hypotheses testing and the assignment of historical type specimens in the genus Callirhinus Blanchard (Coleoptera: Scarabaeidae: Anomalini)
Authors: Ponce Andrés Ramírez , Caballero Santiago Zaragoza , Seidel Matthias
Published: December 2023

Assignation of old type specimens is important for nomenclatural stability, but when it comes to ancient specimens, the use of certain techniques is sometimes not appropriate, due to the condition or age of the specimen. In this study, the supposedly polymorphic and monotypic genus Callirhinus Blanchard was analysed with geometric morphometric techniques and photographs of the pronotum, with the purpose of identifying the locality of the old type specimen (19th century) and to test a priori defined hypothetical species by cumulation of evidence within an integrative taxonomic protocol using geometric morphometry, complemented by morphology and geographical discontinuity. Five morphotypes were defined a priori, and principal component, canonical variates analysis, and distances tests for the assignment of the type specimen based on canonical scores and Procrustes distances between means of groups were carried out. Statistical significance was found in four axes validating the species hypotheses, discovering that the type specimen belongs to the morphotype from Jalisco state. Additionally, a taxonomic treatment, the description of four new species, C. choperi Ramírez-Ponce & Zaragoza sp. nov., C. nanduRamírez-Ponce & Zaragoza sp. nov., C. huiinisRamírez-Ponce & Zaragoza sp. nov., and C. veemeRamírez-Ponce & Zaragoza sp. nov. , keys for the species determination, new distributional records, and a map are provided. The present work demonstrates that Geometric Morphometry is an adequate analytical tool that supports taxonomic identification at low cost and without damaging historical specimens.
Characterization of metabolic phenotypes and distinctive genes in mice with low‐weight gain
Authors: Nemoto Shino , Kubota Tetsuya , Ishikura Tomoyuki , Nakayama Manabu , Kobayashi Atsuo , Yazaki Junshi , Uchida Kazuyo , Matsuda Masashi , Kondo Takashi , Ohara Osamu , Koseki Haruhiko , Koyasu Shigeo , Ohno Hiroshi
Published: December 2023

Being overweight exacerbates various metabolic diseases, necessitating the identification of target molecules for obesity control. In the current study, we investigated common physiological features related to metabolism in mice with low weight gain: (1) G protein‐coupled receptor, family C, group 5, member B‐knockout; (2) gastric inhibitory polypeptide receptor‐knockout; and (3) Iroquois‐related homeobox 3‐knockout. Moreover, we explored genes involved in metabolism by analyzing differentially expressed genes (DEGs) between low‐weight gain mice and the respective wild‐type control mice. The common characteristics of the low‐weight gain mice were low inguinal white adipose tissue (iWAT) and liver weight despite similar food intake along with lower blood leptin levels and high energy expenditure. The DEGs of iWAT, epididymal (gonadal) WAT, brown adipose tissue, muscle, liver, hypothalamus, and hippocampus common to these low‐weight gain mice were designated as candidate genes associated with metabolism. One such gene tetraspanin 7 (Tspan7) from the iWAT was validated using knockout and overexpressing mouse models. Mice with low Tspan7 expression gained more weight, while those with high Tspan7 expression gained less weight, confirming the involvement of the Tspan7 gene in weight regulation. Collectively, these findings suggest that the candidate gene list generated in this study contains potential target molecules for obesity regulation. Further validation and additional data from low‐weight gain mice will aid in understanding the molecular mechanisms associated with obesity.
An insight into genes responsible for fosfomycin resistance among uropathogens of asymptomatic bacteriuria during pregnancy: A North Indian study
Authors: Khatoon Sajda , Sultan Asfia , Khan Fatima , Khan Tamkin , Singh Anuradha
Published: December 2023

Purpose. Asymptomatic bacteriuria (ASB) is a common finding during pregnancy. Effective antibiotic treatment could reduce its adverse effects on both mother and fetus. However, emerging antimicrobial resistance limits the treatment options. Fosfomycin might be a promising drug in this regard, as its resistance is still low. The aim of the study was to determine the antimicrobial susceptibility pattern of fosfomycin in isolates causing ASB by disc diffusion and agar dilution (in selected isolates), determine minimum inhibitory contribution (MIC) by agar dilution in isolates resistant by disc diffusion and detect the genes responsible for fosfomycin resistance.

Methods. This was a 2-year study carried in the Department of Microbiology, Jawaharlal Nehru Medical College and Hospital (JNMCH), Aligarh Muslim University (AMU), Aligarh. A total of 10 252 urine samples from asymptomatic pregnant females (18–45 years) attending the antenatal care (ANC) outpatient department (OPD) were submitted. Identification of pathogen and antimicrobial susceptibility testing (AST) was carried out as per standard methods of CLSI. There was phenotypic detection of methicillin-resistant

Staphylococcus aureus

(MRSA) and other Staphylococcus species (MRSS), high-level aminoglycoside resistance (HLAR), vancomycin resistant Enterococci (VRE) and

S. aureus

(VRSA), extended spectrum β-lactamase (ESBL) and carbapenem-resistant Enterobacterales (CRE). All the fosfomycin-resistant isolates (by disk diffusion) were tested by agar dilution. Conventional PCR was performed for murA, fosA, uhpT and glpT genes on all resistant isolates.

Result. In this study, the prevalence of ASB among pregnant females was 1173(11.4 %), in which

Escherichia coli

495(42 %) was the predominant organism. The overall sensitivity of fosfomycin among Gram-positive cocci (GPC) and Gram-negative bacilli (GNB) was 99 % and 97.6 %, respectively. MRSA and MRSS accounted for 50 (66.6 %) and 71 (76 %), respectively. The highest rates of MIC >2048 µg ml−1 were shown by most isolates (mainly

E. coli

) on agar dilution. PCR studies revealed four

E. coli

strains possessed both murA (also present in one

K. pneumoniae

strain) and glpT genes. While only one isolate (

E. faecalis

) was positive for fosA gene. But none of the strain possessed the uhpT gene.

Conclusion. According to this study, murA and glpT genes were more frequent than fosA. We cannot comment on the prevalence and regional distribution of fosfomycin-resistant genes based on this preliminary study. Therefore, more Indian studies should be carried out to create awareness about the presence of genes in a particular area.