bluuebunny/crossref_metadata_2025 huggingface dataset:
Efficacy and safety of acupuncture treatment for fatigue after COVID-19 infection: study protocol for a pilot randomized sham-controlled trial
Authors: Kim Sung-A. , Lee Ji-Su , Kim Taegon , Kim Tae-Hun , Kwon Sunoh , Kang Jung Won
Published: November 2023

Background

As the coronavirus disease 2019 (COVID-19) pandemic has spread globally, its sequelae, called Long COVID, have persisted, troubling patients worldwide. Although fatigue is known to be the most frequent among Long COVID symptoms, its mechanism and treatment have not been clearly demonstrated. In 2022, we conducted a preliminary prospective case series and found that acupuncture and moxibustion were feasible interventions for fatigue. This study is a pilot patient-assessor-blinded randomized sham-controlled trial to evaluate the efficacy and safety of acupuncture treatment for patients with fatigue that has persisted for at least 4 weeks after recovery from COVID-19.

Methods

Thirty patients will be recruited and randomly assigned to either the acupuncture or sham acupuncture treatment groups. Treatment will be conducted thrice a week for both groups during 4 weeks. The primary outcome will be the efficacy and safety of acupuncture, including numeric rating scale (NRS), brief fatigue inventory (BFI), fatigue severity scale (FSS), and adverse event evaluation. Secondary outcomes will be evaluation of improvement in the comorbid symptoms of fatigue and feasibility variables. Outcome variables will be assessed before treatment, 4 weeks after treatment, and 8 weeks after treatment completion.

Discussion

The results of this study will be used to clarify the efficacy and safety of acupuncture treatment for persistent fatigue in patients with Long COVID. Additionally, the feasibility of the study design was validated to provide evidence for future full-scale randomized controlled trials.

Clinical trial registration: identifier: KCT0008656 https://cris.nih.go.kr/cris/search/detailSearch.do?seq=24785&search_page=L.
Exploring the arcuate fasciculus from a clinical perspective
Authors: Shao Zhi Ding , Gong Yu Juan , Ren Jing , Wang Ji
Published: November 2023

In recent years, language function impairment caused by intracranial diseases has gained increasing interest, mainly due to its significant impact on the language and cognitive ability, leading to a serious decline in the quality of life of patients. Consequently, researchers aimed to clarify the quantitative degree of lesions of the arcuate fasciculus and therapeutic targets to promote nerve fiber remodeling. The arcuate fasciculus is extremely prone to damage caused by diseases such as stroke and brain tumor. Hallucinating schizophrenia, autism spectrum disorder, epilepsy, chronic fatigue syndrome, chronic tinnitus, and other diseases can also lead to changes in the fractional anisotropy value of arcuate fasciculus; however, different studies have different conclusions about how this change occurs. To obtain a better understanding, more clinical studies are required. Owing to various advancements in neuroimaging, a better understanding and identification of vital targets for restoration of neurological function are possible. The arcuate fasciculus is stratified into three substructures, each having unique neurological functions. Both diffusion tensor imaging (DTI) sequences and deterministic monitoring techniques render it possible to visually and quantitatively analyze the substructure in three parts. In this review, we examined the progress of the arcuate fasciculus and quantitative DTI technology in recent years.
Quantitative study of bioinformatics analysis on glioma: a bibliometric analysis
Authors: Yang Xiaobing , Man Dulegeqi , Zhao Peng , Li Xingang
Published: November 2023

Background

The bioinformatics analysis on glioma has been a hot point recently. The purpose of this study was to provide an overview of the research in this field using a bibliometric method.

Methods

The Web of Science Core Collection (WOSCC) database was used to search for literature related to the bioinformatics analysis of gliomas. Countries, institutions, authors, references, and keywords were analyzed using VOSviewer, CiteSpace, and Microsoft Excel software.

Result

China was the most productive country, while the USA was the most cited. Capital Medical University had the largest number of publications and citations. Institutions tend to collaborate more with other institutions in their countries rather than foreign ones. The most productive and most cited author was Jiang Tao. Two citation paths were identified, with literature in basic research journals often cited in clinical journals. Immune-related vocabularies appeared frequently in recent studies.

Conclusion

Glioma bioinformatics analyses spanned a wide range of fields. The international communication in this field urgently needs to be strengthened. Glioma bioinformatics approaches are developing from basic research to clinical applications. Recently, immune-related research has become a focus.
The advantage of point-of-care ultrasound in central venous catheterization and related pericardial effusion in infants in the NICU
Authors: Zhang Yahui , Yan Aijing , Liu Yunfeng
Published: November 2023

Background

Central venous catheterization (CVC) is broadly used in neonatal intensive care units (NICUs) for efficient vascular access; however, its establishment and maintenance are associated with numerous risks and complications. Here, we focus on investigating the value of point-of-care ultrasound (POCUS) in the early diagnosis and treatment of pericardial effusion associated with CVC and compare the differences in ultrasound and radiography in CVC localization and monitoring in the NICU.

Methods

Twenty-five infants with CVC-associated pericardial effusion (PCE) who were hospitalized in the NICU of Peking University Third Hospital between January 2013 and March 2023 were retrospectively selected for the study. Data concerning their catheterization characteristics, CVC tip position, clinical and imaging manifestations of PCE, treatments, and prognoses were analyzed.

Results

The mean gestational age of our cohort was 29.3 ± 3.1 weeks, and the mean birth weight was 1,211 ± 237 g. The incidence of CVC-associated PCE was 0.65%, and 80% of PCE cases occurred within 4 days of CVC. After PCE, the most common symptoms were tachypnea (44%) and tachycardia (64%). Chest radiographs revealed cardiothoracic enlargement, and only 2 cases (9.10%) showed a “flask heart”. Cardiac ultrasound showed that the catheter tip extended deep into the heart in 72% of infants with PCE. Cardiac insufficiency was observed in 12 cases (48%). Overall, 8 infants (32%) had pericardial tamponade, 7 (87.5%) of whom underwent pericardiocentesis. Overall, 2 (8%) infants died, and the remaining 23 (92%) were cured.

Conclusion

CVC-associated PCE mostly occurs in the early post-catheterization stages (within 4 days) in infants. Some cases may have critical clinical manifestations and progress rapidly, with some even developing pericardial tamponade. A CVC tip being deep into the heart cavity is an important cause of PCE. Compared with chest radiography, point-of-care ultrasound is more accurate for CVC tip positioning and can detect PCE more quickly. Furthermore, it is more advantageous for locating and monitoring CVC-associated PCE. Early identification and diagnosis can effectively reduce fatality rates and improve the prognosis of infants with CVC-associated PCE.
High number of seizures and unconsciousness in patients with SARS-CoV-2 omicron variants: a retrospective study
Authors: Tokuyama Kishin , Kitamura Tsubasa , Maruyama Kazutaka , Toriumi Shun , Murano Yayoi , Yoneoka Daisuke , Nakazawa Tomoyuki , Shimizu Toshiaki
Published: November 2023

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) omicron variants are now a pandemic. There are differences in clinical features in SARS-CoV-2 variants and we conducted this study to assess the clinical features of coronavirus disease (COVID-19) in children with SARS-CoV-2 omicron variants. The study included children with COVID-19 arrivedto Tokyo Metropolitan Toshima Hospital between January 2020 and October 2022. The clinical features of 214 children with SARS-CoV-2 non-omicron variants and 557 children with omicron variants were compared. In the SARS-CoV-2 omicron variant group, more patients had fever, sore throat, nausea and/or vomiting, and seizures and/or disorders of consciousness. In SARS-CoV-2 non-omicron variants, there was only one patient with seizure and/or unconsciousness whereas there were 92 children in omicron variants. Among these 92 patients, 46 (49%) were diagnosed with simple febrile seizures; 23 (25%), with complex febrile seizures; 10 (11%) with status epilepticus; and two (2%) with encephalopathy. Their mean age was 4.0 ± 3.0 years—a wider age distribution than that in other febrile seizures but similar to that in febrile seizures in patients with influenza. SARS-CoV-2 omicron variants are likely to cause seizures and unconsciousness in children and their age distribution was wider than other febrile seizures patients but similar to those in influenza patients. In clinical practice in patients with COVID-19 and influenza, clinicians should be aware of these features.
Detecting early signs in Duchenne muscular dystrophy: comprehensive review and diagnostic implications
Authors: Mercuri Eugenio , Pane Marika , Cicala Gianpaolo , Brogna Claudia , Ciafaloni Emma
Published: November 2023

Despite the early onset of clinical signs suggestive of Duchenne muscular dystrophy (DMD), a diagnosis is often not made until four years of age or older, with a diagnostic delay of up to two years from the appearance of the first symptoms. As disease-modifying therapies for DMD become available that are ideally started early before irreversible muscle damage occurs, the importance of avoiding diagnostic delay increases. Shortening the time to a definite diagnosis in DMD allows timely genetic counseling and assessment of carrier status, initiation of multidisciplinary standard care, timely initiation of appropriate treatments, and precise genetic mutation characterization to assess suitability for access to drugs targeted at specific mutations while reducing the emotional and psychological family burden of the disease. This comprehensive literature review describes the early signs of impairment in DMD and highlights the bottlenecks related to the different diagnostic steps. In summary, the evidence suggests that the best mitigation strategy for improving the age at diagnosis is to increase awareness of the early symptoms of DMD and encourage early clinical screening with an inexpensive and sensitive serum creatine kinase test in all boys who present signs of developmental delay and specific motor test abnormality at routine pediatrician visits.
A systematic review of the proposed etiologies of the 2021–2022 outbreaks of pediatric acute hepatitis of unknown etiology
Authors: Lewis Lauren , van Wylick Carly , Mulder Daniel J.
Published: November 2023

In April 2022, the World Health Organization (WHO) declared a global outbreak of acute hepatitis of unknown etiology (AHUE) with a high risk of severe outcomes, for which various etiologies have been proposed by the literature. This study examines primary reports of pediatric AHUE cases and summarizes the proposed etiologies. This systematic review collected and evaluated published peer-reviewed articles, official data, and clinical reports of AHUE cases that met the WHO working case definition. 19 hypothesized etiologies for AHUE were identified from 36 sources, which fell into eight categories. While human adenovirus (HAdV) infection, viral infection, and immune-mediated responses were commonly suspected as causes of AHUE, no definitive etiology or epidemiological link has been established. However, recent evidence implicates adeno-associated virus-2 (AAV2) as a likely significant contributor. Conducting a comprehensive literature review following outbreaks is necessary for developing responsive strategies and protocols.
Simira cordifolia protects against metal induced-toxicity in Caenorhabditis elegans
Authors: Duran-Izquierdo Margareth , Sierra-Marquez Lucellys , Taboada-Alquerque Maria , Olivero-Verbel Jesus
Published: November 2023

Simira cordifolia (Hook.f.) Steyerm (Rubiaceae) is a vascular plant used in Northern Colombia as a source of pigments and wood. However, there is a lack of information regarding its pharmacology and toxicity. This research aimed to study the hydroalcoholic extract of Simira cordifolia as a protector against metal-induced toxicity in Caenorhabditis elegans. Preliminary phytochemical screening of the hydroalcoholic extract of S. cordifolia (HAE-Sc) was conducted using HPLC-ESI-QTOF. Wild-type N2 C. elegans larvae were exposed to different concentrations of HAE-Sc evaluating lethality (50–5000 μg/mL), growth, lifespan, resistance to heat stress, and its protective effect against Mercury (Hg)-, Lead (Pb)- and Cadmium (Cd)-induced lethality (50–1000 μg/mL). The main metabolites present in the extract were iridoids, β-carboline-alkaloids and polyphenols. Bioassays demonstrated that HAE-Sc exhibited low toxicity, with significant lethality (4.2% and 9.4%) occurring at 2500–5000 μg/mL. Growth inhibition reached up to 23.3%, while reproduction declined 13% and 17% at concentrations 500 and 1000 μg/mL, respectively. HAE-Sc enhanced the survival rate of the nematode under thermal stress by up to 79.8%, and extended the mean lifespan of worms by over 33% compared to control. The average lifespan was prolonged by 15.3% and 18.5% at 50 and 100 μg/mL HAE-Sc, respectively. The extract (1000 μg/mL) was able to reduce the death of C. elegans in the presence of heavy metals up to 65.9, 96.8% and 87% for Pb, Hg, and Cd, respectively. In summary, S. cordifolia shows potential protective effects in C. elegans against toxicity caused by heavy metals and heat.
Efficacy and safety of sedation with dexmedetomidine in adults undergoing gastrointestinal endoscopic procedures: systematic review and meta-analysis of randomized controlled trials
Authors: Tang Rou , Huang Yaqun , Zhang Yujia , Ma Xiaolei , Yu Haoyang , Song Kaichao , Ren Ling , Zhao Bin , Wang Lulu , Zheng Wensheng
Published: November 2023

Background: The sedative role of dexmedetomidine (DEX) in gastrointestinal endoscopic procedures is unclear. We performed this systematic review and meta-analysis to assess the efficacy and safety of sedation with DEX during gastrointestinal endoscopic procedures with a view to providing evidence-based references for clinical decision-making.

Methods: The PubMed, Embase, Cochrane Library, Web of Science, and ClinicalTrials.gov databases were searched for randomized controlled trials (RCTs) that compared DEX with different sedatives comparators (such as propofol, midazolam, and ketamine) for sedation in a variety of adult gastrointestinal endoscopic procedures from inception to 1 July 2022. Standardized mean difference (SMD) and weighted mean difference (WMD) with 95% confidence interval (CI) or pooled risk ratios (RR) with 95% CI were used for continuous outcomes or dichotomous outcomes, respectively, and a random-effect model was selected regardless of the significance of the heterogeneity.

Results: Forty studies with 2,955 patients were assessed, of which 1,333 patients were in the DEX group and 1,622 patients were in the control (without DEX) group. The results suggested that the primary outcomes of sedation level of DEX are comparable to other sedatives, with similar RSS score and patient satisfaction level, and better in some clinical outcomes, with a reduced risk of body movements or gagging (RR: 0.60; 95% CI: 0.37 to 0.97; p = 0.04; I2 = 68%), and a reduced additional requirement for other sedatives, and increased endoscopist satisfaction level (SMD: 0.41; 95% CI: 0.05 to 0.77; p = 0.03; I2 = 86%). In terms of secondary outcomes of adverse events, DEX may benefit patients in some clinical outcomes, with a reduced risk of hypoxia (RR:0.34; 95% CI: 0.20 to 0.55; p < 0.0001; I2 = 52%) and cough (RR: 0.25; 95% CI: 0.12 to 0.54; p = 0.0004; I2 = 0%), no significant difference in the risk of hypotension, while an increased risk of bradycardia (RR: 3.08; 95% CI: 2.12 to 4.48; p < 0.00001; I2 = 6%).

Conclusion: This meta-analysis indicates that DEX is a safe and effective sedative agent for gastrointestinal endoscopy because of its benefits for patients in some clinical outcomes. Remarkably, DEX is comparable to midazolam and propofol in terms of sedation level. In conclusion, DEX provides an additional option in sedation for gastrointestinal endoscopic procedures.

Systematic Review Registration:https://www.crd.york.ac.uk/PROSPERO/#searchadvanced
A retrospective case-control study to evaluate the use of beta-lactam desensitization in the management of penicillin-allergic patients: a potential strategy for Antimicrobial Stewardship Programs
Authors: Rodríguez-Alarcón Alicia , Sanz de Mena Manuela , Alanti Soukaina Sara , Echeverría-Esnal Daniel , Sorli Luisa , Sendra Elena , Benítez-Cano Adela , Membrilla Estela , Cots Francesc , Güerri-Fernández Robert , Adalia Ramón , Horcajada Juan Pablo , Escolano Fernando , Grau Santiago , Gómez-Zorrilla Silvia
Published: November 2023

Introduction: Penicillin allergy labels (PAL) are common in the hospital setting and are associated with worse clinical outcomes. Desensitization can be a useful strategy for allergic patients when alternative options are suboptimal or not available. The aim was to compare clinical outcomes of patients with PAL managed with antibiotic desensitization vs. those who received alternative non-beta-lactam antibiotic treatments.

Methods: A retrospective 3:1 case-control study was performed between 2015–2022. Cases were adult PAL patients with infection who required antibiotic desensitization; controls were PAL patients with infection managed with an alternative antibiotic treatment. Cases and controls were adjusted for age, sex, infection source, and critical or non-critical medical services.

Results: Fifty-six patients were included: 14 in the desensitization group, 42 in the control group. Compared to the control group, desensitized PAL patients had more comorbidities, with a higher Charlson index (7.4 vs. 5; p = 0.00) and more infections caused by multidrug-resistant (MDR) pathogens (57.1% vs. 28.6%; p = 0.05). Thirty-day mortality was 14.3% in the desensitized group, 28.6% in the control group (p = 0.24). Clinical cure occurred in 71.4% cases and 54.8% controls (p = 0.22). Four control patients selected for MDR strains after alternative treatment; selection of MDR strains did not occur in desensitized patients. Five controls had antibiotic-related adverse events, including Clostridioides difficile or nephrotoxicity. No antibiotic-related adverse events were found in the study group. In multivariate analysis, no differences between groups were observed for main variables.

Conclusion: Desensitization was not associated with worse clinical outcomes, despite more severe patients in this group. Our study suggests that antibiotic desensitization may be a useful Antimicrobial Stewardship tool for the management of selected PAL patients.
Research on sweat metabolomics of athlete’s fatigue induced by high intensity interval training
Authors: Meihua Su , Jiahui Jin , Yujia Li , Shuang Zhao , Jingjing Zhan
Published: November 2023

Objective: Sweat is an important specimen of human metabolism, which can simply and non-invasively monitor the metabolic state of the body, and its metabolites can be used as biomarkers for disease diagnosis, while the changes of sweat metabolites before and after exercise-induced fatigue are still unclear.

Methods: In this experiment, high-performance chemical isotope labeling liquid chromatography-mass spectrometry (LC-MS) was used to metabolomic 28 sweat samples before and after exercise-induced fatigue of 14 long-distance runners, also IsoMS PRO and SPSS22.0 software were used to analyze the metabolite changes and differential metabolic pathways.

Results: A total of 446 metabolites with high confidence were identified, and the sweat metabolome group before and after high-intensity interval exercise-induced fatigue was obvious, among which the upregulated differential metabolites mainly included hypoxanthine, pyruvate, several amino acids, etc., while the downregulated differential metabolites mainly included amino acid derivatives, vitamin B6, theophylline, etc.

Conclusion: The change of hypoxanthine concentration in sweat can be used as a good biomarker for the diagnosis of exercise-induced fatigue, while the change of pyruvate content in sweat can be used as a discriminant index for the energy metabolism mode of the body before and after exercise. The main metabolic pathways involved in differential metabolites produced before and after HIIT exercise-induced fatigue are purine metabolism and amino acid metabolism.
Optimising clonal performance in sugarcane: leveraging non-additive effects via mate-allocation strategies
Authors: Yadav Seema , Ross Elizabeth M. , Wei Xianming , Powell Owen , Hivert Valentin , Hickey Lee T. , Atkin Felicity , Deomano Emily , Aitken Karen S. , Voss-Fels Kai P. , Hayes Ben J.
Published: November 2023

Mate-allocation strategies in breeding programs can improve progeny performance by harnessing non-additive genetic effects. These approaches prioritise predicted progeny merit over parental breeding value, making them particularly appealing for clonally propagated crops such as sugarcane. We conducted a comparative analysis of mate-allocation strategies, exploring utilising non-additive and heterozygosity effects to maximise clonal performance with schemes that solely consider additive effects to optimise breeding value. Using phenotypic and genotypic data from a population of 2,909 clones evaluated in final assessment trials of Australian sugarcane breeding programs, we focused on three important traits: tonnes of cane per hectare (TCH), commercial cane sugar (CCS), and Fibre. By simulating families from all possible crosses (1,225) with 50 progenies each, we predicted the breeding and clonal values of progeny using two models: GBLUP (considering additive effects only) and extended-GBLUP (incorporating additive, non-additive, and heterozygosity effects). Integer linear programming was used to identify the optimal mate-allocation among selected parents. Compared to breeding value-based approaches, mate-allocation strategies based on clonal performance yielded substantial improvements, with predicted progeny values increasing by 57% for TCH, 12% for CCS, and 16% for fibre. Our simulation study highlights the effectiveness of mate-allocation approaches that exploit non-additive and heterozygosity effects, resulting in superior clonal performance. However, there was a notable decline in additive gain, particularly for TCH, likely due to significant epistatic effects. When selecting crosses based on clonal performance for TCH, the inbreeding coefficient of progeny was significantly lower compared to random mating, underscoring the advantages of leveraging non-additive and heterozygosity effects in mitigating inbreeding depression. Thus, mate-allocation strategies are recommended in clonally propagated crops to enhance clonal performance and reduce the negative impacts of inbreeding.
Solving the mystery of Obake rice in Africa: population structure analyses of Oryza longistaminata reveal three genetic groups and evidence of both recent and ancient introgression with O. sativa
Authors: Labroo Marlee R. , Clark Lindsay V. , Zhang Shilai , Hu Fengyi , Tao Dayun , Hamilton Ruaraidh Sackville , Sacks Erik J.
Published: November 2023

The undomesticated rice relative Oryza longistaminata is a valuable genetic resource for the improvement of the domesticated Asian rice, Oryza sativa. To facilitate the conservation, management, and use of O. longistaminata germplasm, we sought to quantify the population structure and diversity of this species across its geographic range, which includes most of sub-Saharan Africa, and to determine phylogenetic relationships to other AA-genome species of rice present in Africa, including the prevalence of interspecific hybridization between O. longistaminata and O. sativa. Though past plant breeding efforts to introgress genes from O. longistaminata have improved biotic stress resistance, ratooning ability, and yield in O. sativa, progress has been limited by substantial breeding barriers. Nevertheless, despite the strong breeding barriers observed by plant breeders who have attempted this interspecific cross, there have been multiple reports of spontaneous hybrids of O. sativa and O. longistaminata (aka “Obake”) obtained from natural populations in Africa. However, the frequency and extent of such natural introgressions and their effect on the evolution of O. longistaminata had not been previously investigated. We studied 190 O. longistaminata accessions, primarily from the International Rice Research Institute genebank collection, along with 309 O. sativa, 25 Oryza barthii, and 83 Oryza glaberrima control outgroups, and 17 control interspecific O. sativa/O. longistaminata hybrids. We analyzed the materials using 178,651 single-nucleotide polymorphisms (SNPs) and seven plastid microsatellite markers. This study identified three genetic subpopulations of O. longistaminata, which correspond geographically to Northwestern Africa, Pan-Africa, and Southern Africa. We confirmed that O. longistaminata is, perhaps counterintuitively, more closely related to the Asian species, O. sativa, than the African species O. barthii and O. glaberrima. We identified 19 recent spontaneous interspecific hybrid individuals between O. sativa and O. longistaminata in the germplasm sampled. Notably, the recent introgression between O. sativa and O. longistaminata has been bidirectional. Moreover, low levels of O. sativa alleles admixed in many predominantly O. longistaminata accessions suggest that introgression also occurred in the distant past, but only in Southern Africa.
Combining contour and region for closed boundary extraction of a shape
Authors: Hii Doreen , Pizlo Zygmunt
Published: November 2023

This study explored human ability to extract closed boundary of a target shape in the presence of noise using spatially global operations. Specifically, we investigated the contributions of contour-based processing using line edges and region-based processing using color, as well as their interaction. Performance of the subjects was reliable when the fixation was inside the shape, and it was much less reliable when the fixation was outside. With fixation inside the shape, performance was higher when both contour and color information were present compared to when only one of them was present. We propose a biologically-inspired model to emulate human boundary extraction. The model solves the shortest (least-cost) path in the log-polar representation, a representation which is a good approximation to the mapping from the retina to the visual cortex. Boundary extraction was framed as a global optimization problem with the costs of connections calculated using four features: distance of interpolation, turning angle, color similarity and color contrast. This model was tested on some of the conditions that were used in the psychophysical experiment and its performance was similar to the performance of subjects.
The relationships among nature connectedness, climate anxiety, climate action, climate knowledge, and mental health
Authors: Thomson Emily E. , Roach Sean P.
Published: November 2023

Introduction

Climate change is a source of global concern that has both direct and general impacts on mental health. A recent study conducted following severe bushfires in Australia demonstrated relationships among nature connectedness, climate action, climate worry, and mental health; for example, nature connectedness was associated with climate worry, which in turn was associated with psychological distress.

Methods

The present study sought to replicate those findings while building on them in two important ways: on those findings in two ways: first, test similar relationships in a different geographical context that has been mostly spared from direct impacts by acute climate events; second, we take into consideration an additional factor, climate knowledge, which has been linked to relevant factors such as climate anxiety.

Results

The results of a survey completed by 327 adults revealed a similar relationship between nature connectedness and climate anxiety, and between that and psychological distress. Further mirroring those previous findings, nature connectedness was associated with both individual and collective climate action, but the relationships between them and psychological distress differed.

Discussion

The proposed model was a better fit to the collected data among those with high levels of climate change knowledge than those with low levels, suggesting that such knowledge influences how the above factors relate to each other.
Implicit versus explicit processing of visual, olfactory, and multimodal landmark information in human wayfinding
Authors: Schwarz Mira , Hamburger Kai
Published: November 2023

Despite the predominant focus on visual perception in most studies, the role of humans’ sense of smell in navigation has often been neglected. Recent research, however, could show that humans are indeed able to use their sense of smell for orientation, particularly when processed implicitly. In this study, we investigate whether implicit perception of olfactory landmarks enhanced wayfinding performance compared to explicit perception. Fifty-two people completed a wayfinding and a recognition task in a virtual maze at two times of testing 1 month apart. Participants either received olfactory, visual, or both cues at the intersections. Wayfinding performance was better for olfactory landmarks, which were not correctly remembered in the recognition task. In contrast, wayfinding performance was better when visual landmarks were correctly remembered. In the multimodal condition, wayfinding performance was better with landmarks being remembered at t1 and remained the same at t2. Our results suggest distinct implicit processing mechanisms within the olfactory system and therefore hold important implications for the nature of spatial odor processing extending beyond explicit odor localization tasks. The study highlights the importance for future studies to develop and employ further experimental methods that capture implicit processing across all of our senses. This is crucial for a comprehensive understanding of consciousness, as olfaction strongly influences our behavior, but remains largely latent unless deliberately honed through practice.
A search-based geographic metadata curation pipeline to refine sequencing institution information and support public health
Authors: Zhao Kun , Farrell Katie , Mashiku Melchizedek , Abay Dawit , Tang Kevin , Oberste M. Steven , Burns Cara C.
Published: November 2023

Background

The National Center for Biotechnology Information (NCBI) Sequence Read Archive (SRA) has amassed a vast reservoir of genetic data since its inception in 2007. These public data hold immense potential for supporting pathogen surveillance and control. However, the lack of standardized metadata and inconsistent submission practices in SRA may impede the data’s utility in public health.

Methods

To address this issue, we introduce the Search-based Geographic Metadata Curation (SGMC) pipeline. SGMC utilized Python and web scraping to extract geographic data of sequencing institutions from NCBI SRA in the Cloud and its website. It then harnessed ChatGPT to refine the sequencing institution and location assignments. To illustrate the pipeline’s utility, we examined the geographic distribution of the sequencing institutions and their countries relevant to polio eradication and categorized them.

Results

SGMC successfully identified 7,649 sequencing institutions and their global locations from a random selection of 2,321,044 SRA accessions. These institutions were distributed across 97 countries, with strong representation in the United States, the United Kingdom and China. However, there was a lack of data from African, Central Asian, and Central American countries, indicating potential disparities in sequencing capabilities. Comparison with manually curated data for U.S. institutions reveals SGMC’s accuracy rates of 94.8% for institutions, 93.1% for countries, and 74.5% for geographic coordinates.

Conclusion

SGMC may represent a novel approach using a generative AI model to enhance geographic data (country and institution assignments) for large numbers of samples within SRA datasets. This information can be utilized to bolster public health endeavors.
The impact of industry-university-research projects on biopharmaceutical companies’ innovation performance: moderating roles of government subsidies for innovation
Authors: Xia Yuntian , Jia Yiwen
Published: November 2023

Innovation holds paramount importance for both nations and businesses. This article presents a panel regression model designed to assess the fixed effects of industry-university-research (IUR) cooperation projects on innovation performance. Furthermore, it examines the moderating impact of government innovation subsidies by utilizing data spanning from 2007 to 2021, encompassing 326 listed Chinese biopharmaceutical firms. Our findings reveal that industry-university-research-cooperation projects have the potential to significantly enhance innovation performance across three key metrics: input, output, and quality for firms. The presence of government innovation subsidies as a moderator is found to have a positive influence on IUR-cooperation projects and their innovative inputs. However, it can yield adverse effects on IUR-cooperation projects with respect to innovation outputs and quality. The insights presented in this paper introduce innovative recommendations for elevating corporate innovation quality and refining the policies governing IUR cooperation.
The indirect relationship between sleep and cognition in the PREVENT cohort: identifying targets for intervention
Authors: Tari Benjamin , Ben Yehuda Michael , Laurell Axel Anders Stefan , Ritchie Karen , Dauvilliers Yves , Ritchie Craig W. , Lawlor Brian , Naci Lorina , Muniz Terrera Graciela , Malhotra Paresh , Watermeyer Tam , Dudas Robert , Underwood Benjamin R. , O'Brien John T. , Raymont Vanessa , Koychev Ivan
Published: November 2023

Introduction

As the global population ages, the economic, societal, and personal burdens associated with worsening cognition and dementia onset are growing. It is therefore becoming ever more critical to understand the factors associated with cognitive decline. One such factor is sleep. Adequate sleep has been shown to maintain cognitive function and protect against the onset of chronic disease, whereas sleep deprivation has been linked to cognitive impairment and the onset of depression and dementia.

Objectives

Here, we aim to identify and explore mechanistic links between several sleep parameters, depressive symptoms and cognition in a cohort of middle-aged adults.

Methods

We investigated data from the PREVENT dementia programme via structural equation modeling to illustrate links between predictor variables, moderator variables, and two cognitive constructs (i.e., Executive Function and Memory).

Results

Our model demonstrated that sleep quality, and total hours of sleep were related to participants' depressive symptoms, and that, participant apathy was related to higher scores on the Epworth Sleepiness and Lausanne NoSAS Scales. Subsequently, depressive symptoms, but not sleep or apathy ratings, were associated with Executive Function.

Conclusions

We provide evidence for an indirect relationship between sleep and cognition mediated by depressive symptoms in a middle-aged population. Our results provide a base from which cognition, dementia onset, and potential points of intervention, may be better understood.
Be like a panda: reconstructing national identities through China's iconic species
Authors: Guo Yulei , Fennell David , Fennell Sam
Published: November 2023

Despite lacking clear historical significance, the appeal pandas have to the people of China has played an integral role in the emergence of the country's cultural identity and ideals. Few studies have explored the giant panda due to the ongoing dialogue between the West and China, which, according to Edward Said, is permeated with imperialist, colonial, and orientalist flavors. In 1869, the French missionary Armand David encountered a dead specimen of a giant panda in Baoxing, Sichuan, which sparked the beginning of this dialogue. David shipped the skin to Paris, where the animal was named and aesthetically recreated for the first time for Western audiences. In this paper, we approach the giant panda as a dark tourism attraction embodying a process of making and remaking Chinese national identities over the past two centuries. Using “virtual curating” to study the Giant Panda Museum located at the Chengdu Research Base of Giant Panda Breeding, we demonstrate that the giant panda, which has achieved iconic status in China, represents a national history that is dark, backward, and based on suffering and death. We argue that understanding the giant panda's history as a dark tourism attraction provides an ethical vantage point from which to perceive tourist-panda relationships.
On a Scale From “Tom” to “Jerry,” What Is Your Personality Like?
Authors: Henriquez Natacha , Hochheuser Pauline , Kotzamani Marianna , Romanelli Ilaria , Prinzie Peter , Van der Hallen Ruth
Published: November 2023

Meet your new classmates, Tom and Jerry. When you are sad, Jerry is the type of friend that will come up to you and hug you. Tom, on the other hand, is more likely to laugh at you and make you feel silly for feeling sad. Tom and Jerry think and behave differently; that is, they have different personalities. People’s personalities are made up of several personality traits. Some of these personality traits are generally seen as positive (so-called “light” traits) and some are generally seen as negative (so-called “dark” traits), although it is more complicated than that and we all have a mix of both. In this article, we discuss the personalities of Tom and Jerry and the differences and similarities between them. We will also help you to investigate your own personality, to find out if you are more like Tom or Jerry. Lastly, we will talk about how Tom became Tom and how Jerry became Jerry, and whether either of their personalities can change with time.
Early unrestricted vs. partial weight bearing after uncemented total hip arthroplasty: a systematic review and meta-analysis
Authors: Huang Li , Han Weiyu , Qi Weizhong , Zhang Xiaomeng , Lv Zhou , Lu Yao , Zou Danfeng
Published: November 2023

Background

The choice of postoperative weight bearing protocol after uncemented total hip arthroplasty (THA) remains controversial. The aim of this study was to assess the efficacy and safety of immediate unrestricted weight bearing (UWB) compared with partial weight bearing (PWB) in patients undergoing uncemented THA.

Methods

Relevant articles were retrieved from electronic databases. Both randomized controlled trials (RCTs) and non-RCTs were included but analyzed separately. All functional and clinical outcomes with at least 2 independent study outcomes were meta-analyzed.

Results

A total of 17 studies were investigated. No adverse effect was found regarding micromotion of the femoral stem with immediate UWB following uncemented THA. There was also no correlation between immediate UWB and failure of ingrowth fixation and higher risks of femoral stem subsidence and surgical revision in RCTs. Harris hip score was better in patients with immediate UWB than those with PWB at 1 year post surgery, but the difference was not statistically significant.

Conclusions

Immediate UWB did not have extra harm compared with PWB in patients undergoing uncemented THA. UWB was not superior to PWB. Considering the improvement of Harris score and the compliance of patients, UWB can be encouraged in THA rehabilitation.
A new proposal for the use of the focal animal technique in buffaloes in the Eastern Amazon
Authors: Silva Welligton Conceição da , Silva Jamile Andréa Rodrigues da , Gouveia Júnior Amauri , de Alvarenga Adriano Braga Brasileiro , Barbosa Antônio Vinícius Correa , Silva Éder Bruno Rebelo da , Pereira dos Santos Maria Roseane , Lourenço-Júnior José de Brito , Camargo Júnior Raimundo Nonato Colares , Silva André Guimarães Maciel e
Published: November 2023

This study aims to present a proposal for using the focal animal recording technique to evaluate the welfare of buffaloes and to verify the association between each behavior and thermal comfort indices. The study was conducted in an experimental paddock located in Santarém, Pará, Brazil. A total of 10 female Murrah animals were used. The behavior of the animals was recorded during the day, with the use of three trained observers, for 72 consecutive h. Climatic variables were collected, and the Temperature–Humidity Index (THI) and the practical Buffalo Comfort Climatic Conditions Index (BCCCIp) were determined. The multivariate technique of principal components and Spearman’s correlation were employed. BCCCIp and THI were outside the thermal comfort zone at different times of the day. Grazing (P) was more frequent in the coldest hours of the day, while rumination occurred at different periods, mainly during the daytime and frequently in a lying position. There was a positive correlation between idle lying behavior and average temperature—Tmed (r = 0.583; p < 0.028), THI (r = 0.432; p < 0.034), and BCCCIp (r = 0.554; p < 0.049). There was a positive correlation between grazing and Tmed (r = 0.665; p < 0.0004) and BCCCIp (r = 0.583; p < 0.036). The standing idle behavior negatively correlated with Tmed (r = −0.718; p < 0.0001), THI (r = −0.522; p < 0.008), and BCCCIp (r = −0.8076; p < 0.0008). The lying ruminating behavior had a positive correlation with Tmed (r = 0.586; p < 0.002), THI (r = 0.477; p < 0.018), and BCCCIp (r = 0.8033; p < 0.0009). Furthermore, ruminating while standing correlated positively with Tmed (r = 0.680; p < 0.0003). The adaptation of the focal animal technique, with six observers evaluating each animal for 6 min through filming, proved to be efficient in pointing out the different behaviors of buffalo raised in Eastern Amazon fields under heat stress at different times of the day.
The Impact of Agricultural Global Value Chain Participation on Agricultural Total Factor Productivity
Authors: Zhang Defeng , Sun Zhilu
Published: November 2023

Under the condition of opening up, participation in international specialization and global value chains (GVCs) has become the main source for more and more countries to obtain foreign resources and advanced technologies, thereby promoting productivity improvement and technological progress. What are the pathways of agricultural GVC participation that affect agricultural total factor productivity (TFP)? Is the impact of agricultural GVC participation on agricultural TFP consistent across different statuses and modes of agricultural GVC participation? This paper elaborates on the theoretical mechanism of agricultural GVC participation affecting agricultural TFP, and then empirically estimates the impact of different statuses and modes of agricultural GVC participation on agricultural TFP by taking 58 countries as examples. The results show that agricultural GVCs affect agricultural TFP by several direct and indirect pathways. There was a U-shaped relationship between agricultural GVC participation and agricultural TFP, which means that after crossing a certain threshold, the former has a positive impact on the latter. By participating in agricultural GVCs, agricultural TFP in high-income and upper-middle-income countries was significantly improved, while in lower-middle-income countries it was not. Both forward and backward agricultural GVC participation were conducive to improving agricultural TFP in high-income and upper-middle-income countries, but only backward agricultural GVC participation was conducive to improving agricultural TFP in lower-middle-income countries. Therefore, every country needs to actively explore its optimal pathway to participate in agricultural GVCs in order to maximize the participation benefits and promote the improvement in agricultural TFP, simultaneously.
The Influence of Ozonation Carried Out during Vegetation on the Content of Selected Bioactive Phytochemicals and the Microbiological Load of Tubers of Raphanus sativus var. sativus
Authors: Zardzewiały Miłosz , Matłok Natalia , Piechowiak Tomasz , Balawejder Maciej
Published: November 2023

The aim of the study was to determine the effect of ozone gas fumigation on the mechanical, chemical, and microbiological parameters of radish tubers. Radish plants were grown in the ground in accordance with the principles of good agricultural practice and condition suitable for the soil and climatic conditions of south-eastern Poland. At the end of the growing season, 24 h before harvest, radish plants were exposed to a variable factor, i.e., fumigation with ozone gas at various doses (1 ppm for 1 and 5 min; 5 ppm for 1 and 5 min) in order to modify selected metabolic pathways of bioactive compounds. Then, 24 h after ozonation, radish tubers were harvested and placed in a climatic chamber with controlled conditions, i.e., 2 °C and 90% humidity. Laboratory analyses were performed during storage on days 1, 5, and 10. The ozonation used did not significantly improve the mechanical properties and water content of radish tubers. There was a beneficial effect of selected gaseous ozone doses (1 ppm for 1 and 5 min; 5 ppm for 1 min on the 10th day of storage) on the biosynthesis of selected bioactive compounds, i.e., ascorbic acid content, total polyphenol content, and antioxidant potential during storage. The most beneficial effects of the use of gaseous ozone were observed in the storage process in reducing the microbiological load of radish tubers. Among the ozonation doses used, the dose of 5 ppm for 5 min had the most beneficial effect on reducing the microbiological load. It reduced the number of yeasts and molds by 14.2% and aerobic mesophilic bacteria by 20.9% compared to the control sample on the last day of storage. Additionally, between the 5th and 10th day of storage, a significant effect of each ozone dose applied on reducing the occurrence of yeasts, molds, and mesophilic aerobic bacteria during tuber storage was noted.
Effect of Tytanit on Selected Physiological Characteristics, Chemical Composition and Production of Festulolium braunii (K. Richt.) A. Camus
Authors: Sosnowski Jacek , Truba Milena
Published: November 2023

In intensive and sustainable agriculture, it is not enough to use plant protection products and fertilizers, but it is also important to control plant physiological processes. The aim of this study was to determine the effect of Tytanit on Festulolium braunii (K. Richt.) A. Camus dry matter yield, photosynthetic activity and the content of chlorophyll and selected chemical compounds. The pot experiment was conducted in 2019 in a plant breeding room. Four levels of treatment were used: control with no treatment and three stimulant concentrations of 0.02%, 0.04% and 0.06% in the spraying liquid. In particular, the research included the determination of dry weight of plant roots, dry weight of plants, chlorophyll a and b content in leaf blades, maximum and actual efficiency of the leaf photosystem, coefficients of non-photochemical and photochemical fluorescence quenching, and the content of total protein, crude fiber, monosaccharides, crude fat, crude ash, Ca, Mg, P and K in the dry matter of plants. Used in controlled conditions, the stimulant contributed to an increase in most parameter values, increasing photosynthetic activity and the content of chlorophyll a and b, total protein, calcium, magnesium and potassium, but it reduced the amounts of crude fiber.
Social-Ecologically More Sustainable Agricultural Production
Authors: von Cossel Moritz , Castro-Montoya Joaquín , Iqbal Yasir
Published: November 2023

Planet Earth is facing numerous imminent challenges, from climate change to ecological dysfunction, which are largely attributed to anthropogenic activities [...]