bluuebunny/crossref_metadata_2025 huggingface dataset:
Numerical Simulation and Optimization of Highly Stable and Efficient Lead-Free Perovskite FA1−xCsxSnI3-Based Solar Cells Using SCAPS
Authors: Sabbah Hussein , Arayro Jack , Mezher Rabih
Published: July 2022

Formamidinium tin iodide (FASnI3)-based perovskite solar cells (PSCs) have achieved significant progress in the past several years. However, these devices still suffer from low power conversion efficiency (PCE=6%) and poor stability. Recently, Cesium (Cs)-doped Formamidinium tin iodide (FA1−xCsxSnI3) showed enhanced air, thermal, and illumination stability of PSCs. Hence, in this work, FA1−xCsxSnI3 PSCs have been rigorously studied and compared to pure FASnI3 PSCs using a solar cell capacitance simulator (SCAPS) for the first time. The aim was to replace the conventional electron transport layer (ETL) TiO2 that reduces PSC stability under solar irradiation. Therefore, FA1−xCsxSnI3 PSCs with different Cs contents were analyzed with TiO2 and stable ZnOS as the ETLs. Perovskite light absorber parameters including Cs content, defect density, doping concentration and thickness, and the defect density at the interface were tuned to optimize the photovoltaic performance of the PSCs. The simulation results showed that the device efficiency was strongly governed by the ETL material, Cs content in the perovskite and its defect density. All the simulated devices with ZnOS ETL exhibited PCEs exceeding 20% when the defect density of the absorber layer was below 1015 cm−3, and deteriorated drastically at higher values. The optimized structure with FA75Cs25SnI3 as light absorber and ZnOS as ETL showed the highest PCE of 22% with an open circuit voltage Voc of 0.89 V, short-circuit current density Jsc of 31.4 mA·cm−2, and fill factor FF of 78.7%. Our results obtained from the first numerical simulation on Cs-doped FASnI3 could greatly increase its potential for practical production.
Effect of Diamond Burnishing on Fatigue Behaviour of AISI 304 Chromium-Nickel Austenitic Stainless Steel
Authors: Maximov Jordan , Duncheva Galya , Anchev Angel , Dunchev Vladimir , Argirov Yaroslav
Published: July 2022

The disadvantages of widely used austenitic stainless steels are their low hardness and relatively low fatigue strength. Conventional chemical-thermal surface treatments are unsuitable for these steels since they create conditions for inter-granular corrosion. An effective alternative is a low-temperature surface treatment, creating an S-phase within the surface layer, but it has a high cost/quality ratio. Austenitic steels can increase their surface micro-hardness and fatigue strength via surface cold working. When the goal is to increase the rotating bending fatigue strength of austenitic chromium-nickel steels, and the requirements for significant wear resistance are not paramount, diamond burnishing (DB) has significant potential to increase the fatigue strength and, based on the cost/quality ratio, can successfully compete with low-temperature chemical-thermal treatments. The main objective of this study is to establish the effect of DB on the rotating fatigue strength of AISI 304 L chromium-nickel austenitic steel. The influence of DB parameters on the surface integrity (SI) characteristics was studied. Optimal DB parameters under minimum roughness and maximum micro-hardness criteria were obtained. Rotating bending fatigue tests of the diamond burnished (in a different manner) and untreated specimens were performed. DB implemented via parameters providing maximum micro-hardness increased fatigue limit by 38% compared to untreated specimens.
The Plasma Oxylipidome Links Smoking Status to Peripheral Artery Disease
Authors: Caligiuri Stephanie P. B. , Pierce Grant N. , Ravandi Amir , Aukema Harold M.
Published: July 2022

Peripheral artery disease (PAD) is prevalent among individuals with a history of tobacco smoking. Although oxidation of lipids may contribute to atherogenesis in vascular disease, enzymatically and nonenzymatically produced oxidized lipids can have varying and contrasting physiological effects. The underlying mechanisms of atherogenic vulnerability can be better elucidated with the recent advances in oxylipidome quantification using HPLC-MS/MS technology. In a randomized, controlled clinical trial, the plasma oxylipidome was analyzed in participants living with PAD by smoking status (n = 98) and in nonsmoking comparators without chronic disease (n = 20). Individuals with PAD had approximately a four-fold higher level of total plasma oxylipins versus the comparator. Cessation of smoking in individuals with PAD was associated with significantly lower levels of linoleic acid-derived TriHOMEs, greater levels of omega-3 fatty acid-derived oxylipins, and greater levels of nonfragmented oxidized phosphatidylcholines (OxPCs). Individuals living with PAD but without a history of smoking, exhibited higher levels of the putative atherogenic fragmented OxPCs versus individuals who currently or previously smoked. These data implicate the plasma oxylipidome in PAD and that smoking cessation is associated with a less inflammatory profile. Furthermore, fragmented OxPCs may play a more significant role in the pathophysiology of PAD in individuals without a history of smoking.
Application of 3D Bioprinting in Urology
Authors: Zhao Yue , Liu Yuebai , Dai Yi , Yang Luo , Chen Guo
Published: July 2022

Tissue engineering is an emerging field to create functional tissue components and whole organs. The structural and functional defects caused by congenital malformation, trauma, inflammation or tumor are still the major clinical challenges facing modern urology, and the current treatment has not achieved the expected results. Recently, 3D bioprinting has gained attention for its ability to create highly specialized tissue models using biological materials, bridging the gap between artificially engineered and natural tissue structures. This paper reviews the research progress, application prospects and current challenges of 3D bioprinting in urology tissue engineering.
Recent Progress on Optical Tomographic Technology for Measurements and Inspections of Film Structures
Authors: Joo Ki-Nam , Park Hyo-Mi
Published: July 2022

In this review, we present the recent progress on film metrology focused on the advanced and novel technologies during the last two decades. This review consists of various technologies and their measurement schemes to provide the inspiration for understanding each of the measurement principles and applications. In the technology and analysis section, several optical techniques used in film metrology are introduced and described with their benefits and limitations. The temporal, spatial and snapshot measurement schemes of optical film metrology are introduced in the measurement scheme section, and finally, the prospect on optical film metrology will be provided and discussed with the technology trend.
Surface Modification of ZrO2 Nanoparticles with TEOS to Prepare Transparent ZrO2@SiO2-PDMS Nanocomposite Films with Adjustable Refractive Indices
Authors: Cho Hanjun , Lee Deunchan , Hong Suyeon , Kim Heegyeong , Jo Kwanghyeon , Kim Changwook , Yoon Ilsun
Published: July 2022

Here, highly transparent nanocomposite films with an adjustable refractive index were fabricated through stable dispersion of ZrO2 (n = 2.16) nanoparticles (NPs) subjected to surface modification with SiO2 (n = 1.46) in polydimethylsiloxane (PDMS) (n = 1.42) using the Stöber method. ZrO2 NPs (13.7 nm) were synthesized using conventional hydrothermal synthesis, and their surface modification with SiO2 (ZrO2@SiO2 NPs) was controlled by varying the reaction time (3–54 h). The surface modification of the NPs was characterized using Fourier-transform infrared spectroscopy, dynamic light scattering, X-ray photoelectron spectroscopy, scanning electron microscopy, transmission electron microscopy, and ellipsometry. The surface modification was monitored, and the effective layer thickness of SiO2 varied from 0.1 nm to 4.2 nm. The effective refractive index of the ZrO2@SiO2 NPs at λ = 633 nm was gradually reduced from 2.16 to 1.63. The 100 nm nanocomposite film was prepared by spin-coating the dispersion of ZrO2@SiO2 NPs in PDMS on the coverslip. The nanocomposite film prepared using ZrO2@SiO2 NPs with a reaction time of 18 h (ZrO2@SiO2-18h-PDMS) exhibited excellent optical transparency (Taverage = 91.1%), close to the transparency of the coverslip (Taverage = 91.4%) in the visible range, and an adjustable refractive index (n = 1.42–1.60) as the NP content in the film increased from 0 to 50.0 wt%.
Hafnium Oxide Nanostructured Thin Films: Electrophoretic Deposition Process and DUV Photolithography Patterning
Authors: Proust Vanessa , Kirscher Quentin , Nguyen Thi Kim Ngan , Obringer Lisa , Ishii Kento , Rault Ludivine , Demange Valérie , Berthebaud David , Ohashi Naoki , Uchikoshi Tetsuo , Berling Dominique , Soppera Olivier , Grasset Fabien
Published: July 2022

In the frame of the nanoarchitectonic concept, the objective of this study was to develop simple and easy methods to ensure the preparation of polymorphic HfO2 thin film materials (<200 nm) having the best balance of patterning potential, reproducibility and stability to be used in optical, sensing or electronic fields. The nanostructured HfO2 thin films with micropatterns or continuous morphologies were synthesized by two different methods, i.e., the micropatterning of sol-gel solutions by deep ultraviolet (DUV) photolithography or the electrophoretic deposition (EPD) of HfO2 nanoparticles (HfO2-NPs). Amorphous and monoclinic HfO2 micropatterned nanostructured thin films (HfO2-DUV) were prepared by using a sol-gel solution precursor (HfO2-SG) and spin-coating process following by DUV photolithography, whereas continuous and dense monoclinic HfO2 nanostructured thin films (HfO2-EPD) were prepared by the direct EPD of HfO2-NPs. The HfO2-NPs were prepared by a hydrothermal route and studied through the changing aging temperature, pH and reaction time parameters to produce nanocrystalline particles. Subsequently, based on the colloidal stability study, suspensions of the monoclinic HfO2-NPs with morphologies near spherical, spindle- and rice-like shapes were used to prepare HfO2-EPD thin films on conductive indium-tin oxide-coated glass substrates. Morphology, composition and crystallinity of the HfO2-NPs and thin films were investigated by powder and grazing incidence X-ray diffraction, scanning electron microscopy, transmission electron microscopy and UV-visible spectrophotometry. The EPD and DUV photolithography performances were explored and, in this study, it was clearly demonstrated that these two complementary methods are suitable, simple and effective processes to prepare controllable and tunable HfO2 nanostructures as with homogeneous, dense or micropatterned structures.
Activation of Extracellular Signal-Regulated Kinase 2 and cAMP Response Element-Binding Protein in Cultured Neurons by the Macrocyclic Ellagitannin Oenothein B
Authors: Okuyama Satoshi , Yoshimura Morio , Amakura Yoshiaki , Nakajima Mitsunari , Furukawa Yoshiko
Published: July 2022

(1) Background: The findings of our recent in vivo study indicated that the oral administration of oenothein B, a unique macrocyclic ellagitannin, activated extracellular signal-regulated kinase (ERK) 2 and cAMP response element-binding protein (CREB) in the mouse brain. A large hydrophilic oenothein B is unable to reach the brain, suggesting that any metabolite(s) of oenothein B might function in the brain. (2) Results: The addition of oenothein B to the culture medium of rat cortical neurons induced the prompt and significant activation of ERK2 and CREB. (3) Conclusions: The activation of ERK2 and CREB is crucial for synaptic transmission and learning/memory formation in the brain. The present results suggest oenothein B exerts neurotrophic/neuroprotective effects in the brain through the modulation of neuronal signaling pathways, if it reaches the brain.
Arthrospira Enhances Seroclearance in Patients with Chronic Hepatitis B Receiving Nucleos(t)ide Analogue through Modulation of TNF-α/IFN-γ Profile
Authors: Shiue Sheng-Jie , Cheng Chao-Ling , Shiue Han-Shiang , Chen Chun-Nan , Cheng Sheng-Wei , Wu Li-Wei , Jargalsaikhan Ganbolor , Chan Tze-Sian , Lin Hsin-Yi , Wu Ming-Shun
Published: July 2022

Chronic hepatitis B (CHB) virus infection, causing immune dysfunction and chronic hepatitis, is one of the leading risk factors for hepatocellular cancer. We investigated how Arthrospira affected hepatitis B surface antigen (HBsAg) reduction in CHB patients under continued nucleos(t)ide analogues (NA). Sixty CHB patients who had been receiving NA for at least one year with undetectable HBV DNA were randomized into three groups: control and oral Arthrospira at 3 or 6 g daily add-on therapy groups. Patients were followed up for 6 months. Oral Arthrospira-diet mice were established to investigate the possible immunological mechanism of Arthrospira against HBV. Within 6 months, mean quantitative HBsAg (qHBsAg) decreased in the oral Arthrospira add-on therapy group. Interestingly, interferon gamma (IFN-γ) increased but TNF-α, interleukin 6 (IL-6), hepatic fibrosis, and steatosis decreased in the add-on groups. In mice, Arthrospira enhanced both innate and adaptive immune system, especially natural killer (NK) cell cytotoxicity, B cell activation, and the interleukin 2 (IL-2), IFN-γ immune response. Arthrospira may modulate IL-2- and TNF-α/IFN-γ-mediated B and T cell activation to reduce HBsAg. Also, Arthrospira has the potential to restore immune tolerance and enhance HBsAg seroclearance in CHB patients through promoting T, B, and NK cell activation.
Compromised Hindgut Microbial Digestion, Rather Than Chemical Digestion in the Foregut, Leads to Decreased Nutrient Digestibility in Pigs Fed Low-Protein Diets
Authors: Zhou Junyan , Wang Yuming , Wang Lu , Tu Jiayu , Yang Lijie , Yang Guangxin , Zeng Xiangfang , Qiao Shiyan
Published: July 2022

Background: Reduced nutrient digestibility due to low-protein (LP) diets occurring in the foregut or hindgut of pigs remains unclear. Methods: Growing barrows (21.7 ± 1.7 kg) were allotted into LP and high-protein (HP) diet treatments. Ileal digesta and feces were collected for in vitro cross-fermentation and microbial sequencing, and cross-feeding assessed nutrient digestibility. Results: No difference in foregut digesta flora and nutrient digestibility between treatments was observed. LP diet caused decreased total tract digestibility of dry matter (DM), organic matter (OM), gross energy (GE), neutral detergent fiber (NDF), and acid detergent fiber (ADF) compared with the HP diet (p < 0.05). The fermentation broth from LP diet-fed pigs induced less full fermentation digestion of DM, OM, crude protein, and GE than HP broth (p < 0.05). Additionally, LP broth fermentation presented lower fermentation gas and short-chain fatty acids (SCFAs) generation than HP group (p < 0.05). This situation above may be related to decreased abundances of Lachnospiraceae, Eubacterium_eligens_group, Roseburia, and Ruminococcaceae_UCG-009, which can efficiently ferment nutrients to produce SCFA. Conclusions: Change in the flora caused compromise in hindgut microbial fermentation digestion leads to decreased total tract nutrient digestibility in pigs fed an LP diet.
Identification of CD73 as the Antigen of an Antigen-Unknown Monoclonal Antibody Established by Exosome Immunization, and Its Antibody–Drug Conjugate Exerts an Antitumor Effect on Glioblastoma Cell Lines
Authors: Anzai Takahiro , Saijou Shinji , Takashima Hiroki , Hara Misato , Hanaoka Shingo , Matsumura Yasuhiro , Yasunaga Masahiro
Published: July 2022

Development of antibodies against the native structure of membrane proteins with multiple transmembrane domains is challenging because it is difficult to prepare antigens with native structures. Previously, we successfully developed a monoclonal antibody against multi-pass membrane protein TMEM180 by exosome immunization in rats. This approach yielded antibodies that recognized cancer-specific antigens on the exosome. In this study, we performed immunoprecipitation using magnetic beads to identify the antigen of one of the rat antibody clones, 0614, as CD73. We then converted antibody 0614 to human chimeric antibody 0614-5. Glioblastoma (GB) was the cancer type with the highest expression of CD73 in the tumor relative to healthy tissue. An antibody–drug conjugate (ADC) of 0614-5 exerted an antitumor effect on GB cell lines according to expression of CD73. The 0614-5-ADC has potential to be used to treat cancers with high CD73 expression. In addition, our strategy could be used to determine the antigen of any antibody produced by exosome immunization, which may allow the antibody to advance to new antibody therapies.
New Therapeutic Approaches in Treatment of Dyslipidaemia—A Narrative Review
Authors: Merćep Iveta , Strikić Dominik , Slišković Ana Marija , Reiner Željko
Published: July 2022

Dyslipidaemia is a well-known risk factor for the development of cardiovascular disease, a leading cause of morbidity and mortality in developed countries. As a consequence, the medical community has been dealing with this problem for decades, and traditional statin therapy remains the cornerstone therapeutic approach. However, clinical trials have observed remarkable results for a few agents effective in the treatment of elevated serum lipid levels. Ezetimibe showed good but limited results when used in combination with statins. Bempedoic acid has been thoroughly studied in multiple clinical trials, with a reduction in LDL cholesterol by approximately 15%. The first approved monoclonal antibodies for the treatment of dyslipidaemia, PCSK9 inhibitors, are currently used as second-line treatment for patients with unregulated lipid levels on statin or statin combination therapy. A new siRNA molecule, inclisiran, demonstrates great potential, particularly concerning compliance, as it is administered twice yearly and pelacarsen, an antisense oligonucleotide that targets lipoprotein(a) and lowers its levels. Volanesorsen is the first drug that was designed to target chylomicrons and lower triglyceride levels, and olezarsen, the next in-line chylomicron lowering agent, is currently being researched. The newest possibilities for the treatment of dyslipidaemia are ANGPTL3 inhibitors with evinacumab, already approved by the FDA, and EMA for the treatment of familial hypercholesterolemia. This article provides a short summary of new agents currently used or being developed for lipid lowering treatment.
Immunomodulatory Effect of Hispolon on LPS-Induced RAW264.7 Cells and Mitogen/Alloantigen-Stimulated Spleen Lymphocytes of Mice
Authors: Lee Eun Kyeong , Koh Eun Mi , Kim Yu Na , Song Jeongah , Song Chi Hun , Jung Kyung Jin
Published: July 2022

Hispolon is a potent anticancer, anti-inflammatory, antioxidant, and antidiabetic agent isolated from Phellinus linteus, an oriental medicinal mushroom. However, the immunomodulatory mechanisms by which hispolon affects macrophages and lymphocytes remain poorly characterized. We investigated the immunomodulatory effects of hispolon on oxidative stress, inflammatory responses, and lymphocyte proliferation using lipopolysaccharide (LPS)-treated RAW264.7 macrophages or mitogen/alloantigen-treated mouse splenocytes. Hispolon inhibited LPS-induced reactive oxygen and nitrogen species (ROS/RNS) generation and decreased total sulfhydryl (SH) levels in a cell-free system and RAW264.7 cells. Hispolon exerted significant anti-inflammatory effects by inhibiting production of the proinflammatory cytokines interleukin 6 (IL-6) and tumor necrosis factor alpha (TNF-α) and activation of nuclear factor kappa B (NF-κB) and signal transducer and activator of transcription 3 (STAT3) in LPS-treated RAW264.7 cells. Hispolon also modulated NF-κB and STAT3 activation by suppressing the NF-κB p65 interaction with phospho-IκBα and the STAT3 interaction with JAK1, as determined via coimmunoprecipitation analysis. Additionally, hispolon significantly decreased lymphocyte proliferation, T cell responses and T helper type 1 (Th1)/type 2 (Th2) cytokines production in mitogen/alloantigen-treated splenocytes. We conclude that hispolon exerts immunomodulatory effects on LPS-treated macrophages or mitogen/alloantigen-treated splenocytes through antioxidant, anti-inflammatory, and antiproliferative activities. Thus, hispolon may be a therapeutic agent for treating immune-mediated inflammatory diseases.
Translating a Thin-Film Rehydration Method to Microfluidics for the Preparation of a SARS-CoV-2 DNA Vaccine: When Manufacturing Method Matters
Authors: Peletta Allegra , Prompetchara Eakachai , Tharakhet Kittipan , Kaewpang Papatsara , Buranapraditkun Supranee , Yostrerat Nongnaphat , Manopwisedjaroen Suwimon , Thitithanyanont Arunee , Avaro Jonathan , Krupnik Leonard , Neels Antonia , Ruxrungtham Kiat , Ketloy Chutitorn , Borchard Gerrit
Published: July 2022

Previous investigations conducted on a liposomal formulation for a SARS-CoV-2 DNA vaccine manufactured using the thin-film layer rehydration method showed promising immunogenicity results in mice. The adaptation of the liposomal formulation to a scalable and reproducible method of manufacture is necessary to continue the investigation of this vaccine candidate. Microfluidics manufacture shows high potential in method translation. The physicochemical characterization of the blank liposomes produced by thin-film layer rehydration or microfluidics were shown to be comparable. However, a difference in lipid nanostructure in the bilayer resulted in a significant difference in the hydration of the thin-film liposomes, ultimately altering their complexation behavior. A study on the complexation of liposomes with the DNA vaccine at various N/P ratios showed different sizes and Zeta-potential values between the two formulations. This difference in the complexation behavior resulted in distinct immunogenicity profiles in mice. The thin-film layer rehydration-manufactured liposomes induced a significantly higher response compared to the microfluidics-manufactured samples. The nanostructural analysis of the two samples revealed the critical importance of understanding the differences between the two formulations that resulted in the different immunogenicity in mice.
Trans-Cinnamaldehyde Eluting Porous Silicon Microparticles Mitigate Cariogenic Biofilms
Authors: Jailani Afreen , Kalimuthu Shanthini , Rajasekar Vidhyashree , Ghosh Sumanta , Collart-Dutilleul Pierre-Yves , Fatima Naveen , Koo Hyun , Solomon Adline Princy , Cuisinier Frederic , Neelakantan Prasanna
Published: July 2022

Dental caries, a preventable disease, is caused by highly-adherent, acid-producing biofilms composed of bacteria and yeasts. Current caries-preventive approaches are ineffective in controlling biofilm development. Recent studies demonstrate definite advantages in using natural compounds such as trans-cinnamaldehyde in thwarting biofilm assembly, and yet, the remarkable difficulty in delivering such hydrophobic bioactive molecules prevents further development. To address this critical challenge, we have developed an innovative platform composed of components with a proven track record of safety. We fabricated and thoroughly characterised porous silicon (pSi) microparticles to carry and deliver the natural phenyl propanoid trans-cinnamaldehyde (TC). We investigated its effects on preventing the development of cross-kingdom biofilms (Streptococcus mutans and Candida albicans), typical of dental caries found in children. The prepared pSi microparticles were roughly cubic in structure with 70–75% porosity, to which the TC (pSi-TC) was loaded with about 45% efficiency. The pSi-TC particles exhibited a controlled release of the cargo over a 14-day period. Notably, pSi-TC significantly inhibited biofilms, specifically downregulating the glucan synthesis pathways, leading to reduced adhesion to the substrate. Acid production, a vital virulent trait for caries development, was also hindered by pSi-TC. This pioneering study highlights the potential to develop the novel pSi-TC as a dental caries-preventive material.
Downstream Processing of Itraconazole:HPMCAS Amorphous Solid Dispersion: From Hot-Melt Extrudate to Tablet Using a Quality by Design Approach
Authors: Mishra Saurabh M , Richter Margarethe , Mejia Luis , Sauer Andreas
Published: July 2022

The downstream processing of hot-melt extruded amorphous solid dispersions (ASDs) into tablets is challenging due to the low tabletability of milled ASDs. Typically, the extrudate strand is sized before milling, as the strand cannot be fed directly into the milling system. At the lab scale, the strand can be sized by hand-cutting before milling. For scaling up, pelletizers or chill roll and flaker systems can be used to break strands. Due to the different techniques used, differences in milling and tablet compaction are to be expected. We present a systematic study of the milling and tableting of an extruded ASD of itraconazole with hypromellose acetate succinate (HPMCAS) as a carrier polymer. The strand was sized using different techniques at the end of the extruder barrel (hand-cutting, pelletizer, or chill roll and flaker) before being milled at varying milling speeds with varying screen sizes. The effects of these variables (sizing technology, milling speed, and screen size) on the critical quality attributes (CQAs) of the milled ASD, such as yield, mean particle size (D50), tablet compaction characteristics, and tablet dissolution, were established using response surface methodology. It was found that the CQAs varied according to sizing technology, with chill roll flakes showing the highest percentage yield, the lowest D50, and the highest tabletability and dissolution rate for itraconazole. Pearson correlation coefficient tests indicated D50 as the most important CQA related to tabletability and dissolution. For certain milling conditions, the milling of hand-cut filaments results in similar particle size distributions (PSDs) to the milling of pellets or chill roll flakes.
Antifungal and Herbicidal Potential of Piper Essential Oils from the Peruvian Amazonia
Authors: Ruiz-Vásquez Liliana , Ruiz Mesia Lastenia , Caballero Ceferino Henrry Denny , Ruiz Mesia Wilfredo , Andrés Maria Fe , Díaz Carmen Elisa , Gonzalez-Coloma Azucena
Published: July 2022

The chemical composition of essential oils (EOs) from ten Peruvian Piper species (Piper coruscans, Pc; P. tuberculatum, Pt; P. casapiense, Pcs; P. obliquum, Po; P. dumosum, Pd; P. anonifolium, Pa; P. reticulatum, Pr; P. soledadense, Ps; P. sancti-felicis, Psf and P. mituense, Pm) has been studied, along with their antifungal and phytotoxic activities. These EOs contained β-bisabolene/nerolidol (Pc), β-bisabolene/δ-cadinene/caryophyllene (Pt), caryophyllene oxide (Pcs), bicyclogermacrene/10-epi-Elemol (Po), bicyclogermacrene/germacrene-D/apiol (Pd), caryophyllene/germacrene-D (Pa), germacrene-D (Pr), limonene/apiol (Ps), apiol (Psf), and apiol/bicyclogermacrene (Pm) as major components, and some are described here for the first time (Ps, Pcs, Pm). A composition-based dendrogram of these Piper species showed four major groups (G1: Pc and Pt, G2: Pcs, Po, Pd, Pa, and Pr, G3: Ps, and G4: Psf and Pm). The spore germination effects (Aspergillus niger, Botrytis cinerea, and Alternaria alternate) and phytotoxicity (Lolium perenne and Lactuca sativa) of these EOs were studied. Most of these Piper essential oils showed important activity against phytopathogenic fungi (except G1), especially against B. cinerea. Similarly, most of the essential oils were phytotoxic against L. perenne (except G1), with P. sancti-felicis (G4), P. casapiense (G2), and P. reticulatum (G2) being the most effective. Caryophyllene oxide, β-caryophyllene, β-pinene, limonene, α-humulene, and apiol were evaluated against B. cinerea, with the most effective compounds being β-pinene, apiol, and limonene. This work demonstrates the species-dependent potential of essential oils from Peruvian Piper species as fungicidal and herbicidal agents.
Effect of Tecoma stans (L.) Juss. ex Kunth in a Murine Model of Metabolic Syndrome
Authors: Morales-Ferra Dulce Lourdes , Zavala-Sánchez Miguel Ángel , Jiménez-Ferrer Enrique , González-Cortazar Manasés , Zamilpa Alejandro
Published: July 2022

Metabolic syndrome is a constellation of abnormalities related to insulin resistance with an unfortunately high prevalence worldwide. Tecoma stans (L.) Juss. Ex Kunth. is a well-known medicinal plant that has been studied in several biological models related to diabetes mellitus. The aim of this study was to evaluate the effects of T. stans on a hypercaloric diet-induced metabolic syndrome model. An organic fraction obtained using liquid–liquid separation from the hydroalcoholic extract of T. stans and four subfractions of this organic fraction were administered for ten weeks to C57BL6J male mice previously fed with a hypercaloric diet. The hypercaloric diet caused changes in glucose levels (from 65.3 to 221.5 mg/dL), body weight (31.3 to 42.2 g), triglycerides (91.4 to 177.7 mg/dL), systolic (89.9 to 110.3 mmHg) and diastolic (61.6 to 73.7 mg/dL) blood pressure, and insulin resistance (4.47 to 5.16). Treatment with T. stans resulted in improvements in triglycerides (83.4–125.0 mg/dL), systolic blood pressure (75.1–91.8 mmHg), and insulin resistance (4.72–4.93). However, the organic fraction and hydroalcoholic extract produced a better response in diastolic blood pressure (52.8–56.4 mmHg). Luteolin, apigenin, and chrysoeriol were the major constituents in the most active subfractions. Treatment with T. stans, particularly a luteolin-rich organic fraction, achieved an improvement in metabolic syndrome alterations.
Analysis of the RNA-Dependent RNA Polymerase 1 (RDR1) Gene Family in Melon
Authors: Leibman Diana , Pashkovsky Ekaterina , Shnaider Yulia , Shtarkman Meital , Gaba Victor , Gal-On Amit
Published: July 2022

RNA-dependent RNA polymerase 1 (RDR1) plays a crucial defense role against plant viruses by secondary amplification of viral double-stranded RNA in the gene-silencing pathway. In this study, it was found that melon (Cucumis melo) encodes four RDR1 genes (CmRDR1a, b, c1 and c2) similar to the CsRDR1 gene family of cucumber (C. sativus). However, in contrast to cucumber, melon harbors a truncated CmRDR1b gene. In healthy plants, CmRDR1a was expressed, whereas the expression of CmRDR1c1/c2 was not detected. CmRDR1a expression level increased 20-fold upon cucumber mosaic virus (CMV) infection and was not increased in melon plants infected with zucchini yellow mosaic virus (ZYMV), cucumber vein yellowing virus (CVYV) and cucumber green mottle mosaic virus (CGMMV). The expression of CmRDR1c1/c2 genes was induced differentially by infection with viruses from different families: high levels of ~340-, 172- and 115-fold increases were induced by CMV, CVYV and CGMMV, respectively, and relatively low-level increases by potyvirus infection (4- to 6-fold). CMV mutants lacking the viral silencing suppressor 2b protein did not cause increased CmRDR1c/c2 expression; knockout of CmRDR1c1/c2 by CRISPR/Cas9 increased susceptibility to CMV but not to ZYMV. Therefore, it is suggested that the sensitivity of melon to viruses from different families is a result of the loss of function of CmRDR1b.
Characterization of Gels and Films Produced from Pinhão Seed Coat Nanocellulose as a Potential Use for Wound Healing Dressings and Screening of Its Compounds towards Antitumour Effects
Authors: de Lima Tielidy A. de M. , de Lima Gabriel Goetten , Chee Bor Shin , Henn Jeferson G. , Cortese Yvonne J. , Matos Mailson , Helm Cristiane V. , Magalhães Washington L. E. , Nugent Michael J. D.
Published: July 2022

The reuse of agro-industrial waste assumes great importance today. Pinhão is the seed of Araucaria angustifolia, which is native to the mountains of southern Brazil, Paraguay, and Argentina. The coat is a by-product of this seed and is rich in phenolic compounds. The present study aimed to use the residue as a precursor material for the production of nanocellulose through the mechanical defibrillation process and perform the characterization of the films and the gel to investigate the effect on the physical and regenerative properties when incorporated with polyvinyl alcohol (PVA). The modulus of elasticity was higher when the MFC of pinhão was added to the PVA. Film and gel had their cytotoxicity tested by MTT assay using 3T3 fibroblast and Schwann cancer cells, and a migration assay was also performed using the scratch test on HaCat keratinocyte cells. For the scratch test, film and gel samples with low concentration presented a complete scratch closure in 72 h. Molecular docking was performed and quercetin had the ideal interaction score values, so it was used with the PACAP protein which presented a slightly moderate interaction with the protein synthesis of Schwann cells, presenting compactness of the compound after 14 ns.
Photocatalytic CO2 Conversion Using Metal-Containing Coordination Polymers and Networks: Recent Developments in Material Design and Mechanistic Details
Authors: Hornberger Lea-Sophie , Adams Friederike
Published: July 2022

International guidelines have progressively addressed global warming which is caused by the greenhouse effect. The greenhouse effect originates from the atmosphere’s gases which trap sunlight which, as a consequence, causes an increase in global surface temperature. Carbon dioxide is one of these greenhouse gases and is mainly produced by anthropogenic emissions. The urgency of removing atmospheric carbon dioxide from the atmosphere to reduce the greenhouse effect has initiated the development of methods to covert carbon dioxide into valuable products. One approach that was developed is the photocatalytic transformation of CO2. Photocatalysis addresses environmental issues by transferring CO2 into value added chemicals by mimicking the natural photosynthesis process. During this process, the photocatalytic system is excited by light energy. CO2 is adsorbed at the catalytic metal centers where it is subsequently reduced. To overcome several obstacles for achieving an efficient photocatalytic reduction process, the use of metal-containing polymers as photocatalysts for carbon dioxide reduction is highlighted in this review. The attention of this manuscript is directed towards recent advances in material design and mechanistic details of the process using different polymeric materials and photocatalysts.
Titanium or Biodegradable Osteosynthesis in Maxillofacial Surgery? In Vitro and In Vivo Performances
Authors: Gareb Barzi , Van Bakelen Nico B. , Vissink Arjan , Bos Ruud R. M. , Van Minnen Baucke
Published: July 2022

Osteosynthesis systems are used to fixate bone segments in maxillofacial surgery. Titanium osteosynthesis systems are currently the gold standard. However, the disadvantages result in symptomatic removal in up to 40% of cases. Biodegradable osteosynthesis systems, composed of degradable polymers, could reduce the need for removal of osteosynthesis systems while avoiding the aforementioned disadvantages of titanium osteosyntheses. However, disadvantages of biodegradable systems include decreased mechanical properties and possible foreign body reactions. In this review, the literature that focused on the in vitro and in vivo performances of biodegradable and titanium osteosyntheses is discussed. The focus was on factors underlying the favorable clinical outcome of osteosyntheses, including the degradation characteristics of biodegradable osteosyntheses and the host response they elicit. Furthermore, recommendations for clinical usage and future research are given. Based on the available (clinical) evidence, biodegradable copolymeric osteosyntheses are a viable alternative to titanium osteosyntheses when applied to treat maxillofacial trauma, with similar efficacy and significantly lower symptomatic osteosynthesis removal. For orthognathic surgery, biodegradable copolymeric osteosyntheses are a valid alternative to titanium osteosyntheses, but a longer operation time is needed. An osteosynthesis system composed of an amorphous copolymer, preferably using ultrasound welding with well-contoured shapes and sufficient mechanical properties, has the greatest potential as a biocompatible biodegradable copolymeric osteosynthesis system. Future research should focus on surface modifications (e.g., nanogel coatings) and novel biodegradable materials (e.g., magnesium alloys and silk) to address the disadvantages of current osteosynthesis systems.
Catholic Transitions and Tensions: Marriage, Divorce, Plural Normative Standards, and New Paradigms
Authors: Madera Adelaide
Published: July 2022

In the framework of the process of secularization of civil society, the institution of marriage has traditionally been at the crossroads between religious and secular law, and it gives the opportunity to investigate whether and to what degree a religious law can develop when it interacts with a secular juridical context without weakening its identity. The Italian lawmaker has traditionally adopted a “benevolent secularism” approach, trying to balance new social expectations with the Catholic idea of marriage. The above-mentioned approach has resulted in aligning with the mainstream opinions in Italian society, which are consistent with the guidelines of the Catholic Church. Indeed, in Italy, the Catholic Church, which considers marriage as a sacred unbreakable bond between a man and a woman, has often had an open and incisive influence on legislative policy choices. Since 1970, there has a been a gradual erosion of Catholic influence on public policies. New statutes and judicial rulings concerning such issue have emphasized a sharp ideological and political polarization between two opposite ethical narratives: the secular and the religious/Catholic one. Catholic tenets are no more able to influence political democratic processes. In the last fifty years, Italian legislation has followed a more progressive direction with regard to the issue of marriage, taking distances from the Catholic model. The Italian legal system has also started to face controversial issues, such as the status of same-sex unions, recognizing broader rights with a view to guaranteeing the coexistence of multiple views about marriage. Thus, the Catholic Church is challenged by new paradigms and is undergoing deep internal tensions and transitions. The present paper aims to focus on some new challenges, with regard to the status of divorcees who married again, unmarried couples, and same-sex couples in canon law. In the framework of the debate concerning the role and the reformability of religious laws, it will take into consideration new pontifical approaches.
Praying Together, Hating Together—Transforming Hostility through the Effective Direction of Religious Communitarization
Authors: Harfensteller Georg
Published: July 2022

Perceived belonging to a community is one of the most frequently given reasons for the regularity of religious practice. However, it also plays a key role in the practice of religious violence. The paper addresses the relationship between felt belonging, which is established in shared religious experiences, and different expressions of religious hostility. By means of a phenomenological analysis based on the work of Bernhard Waldenfels, the author distinguishes between different modes of religious self-localization. On the one hand, the performative moments of religious practices are reconstructed, unfolding their effect even in regular and less intensive experiences. On the other hand, the intrinsically shared nature of religious assemblies becomes describable, which includes the co-actors in the process of religious self-constitution. Due to the existential and shared nature of this self-constitution, violent affects appear to be group-directed, which particularly encourages the self-sacrificial behavior that seems to distinguish religious violence. This methodological focus on performative communitarization keeps the study also interoperable with a variety of meaning-based theories, promoting phenomenology as a resourceful method for a philosophical division of labor.
Emotion Work in Tabligh Jama’at Texts
Authors: Sahib Rizwan
Published: July 2022

This study examines the emotional dynamics of the written and oral texts of Tabligh Jama’at—respectively, Faza’il-e-A’maal (Virtues of Good Deeds) and bayan (religious sermon). In them, the study identifies emotion work—the attempt to generate certain emotions. The study discusses how the texts’ emotion work relates to Tablighi discursive ideology (framing) and also posits several emotions that the emotion work might generate. From these findings, the study offers the idea that Tablighi emotion work contributes to transforming Muslims’ emotional sphere by attaching them emotionally to ultimate religious concerns. By enchanting Muslims’ emotional sphere and attaching Muslims to Islamic social actors, values, practices, and Islamic revivalist goals, Tablighi emotion work contributes to the social transformation of individuals and society.
A Fast and Close-to-Optimal Receding Horizon Control for Trajectory Generation in Dynamic Environments
Authors: Hoang-Dinh Khoi , Leibold Marion , Wollherr Dirk
Published: July 2022

This paper presents a new approach for the optimal trajectory planning of nonlinear systems in a dynamic environment. Given the start and end goals with an objective function, the problem is to find an optimal trajectory from start to end that minimizes the objective while taking into account the changes in the environment. One of the main challenges here is that the optimal control sequence needs to be computed in a limited amount of time and needs to be adapted on-the-fly. The control method presented in this work has two stages: the first-order gradient algorithm is used at the beginning to compute an initial guess of the control sequence that satisfies the constraints but is not yet optimal; then, sequential action control is used to optimize only the portion of the control sequence that will be applied on the system in the next iteration. This helps to reduce the computational effort while still being optimal with regard to the objective; thus, the proposed approach is more applicable for online computation as well as dealing with dynamic environments. We also show that under mild conditions, the proposed controller is asymptotically stable. Different simulated results demonstrate the capability of the controller in terms of solving various tracking problems for different systems under the existence of dynamic obstacles. The proposed method is also compared to the related indirect optimal control approach and sequential action control in terms of cost and computation time to evaluate the improvement of the proposed method.
Phase Centre Corrections of GNSS Antennas and Their Consistency with ATX Catalogues
Authors: Borowski Lukasz , Kudrys Jacek , Kubicki Bartosz , Slámová Martina , Maciuk Kamil
Published: July 2022

Changes of the antenna models on permanent global navigation satellite system (GNSS) stations can lead to jumps and discontinuities in the coordinate time series. In this paper, the results of research on the adequacy of the antenna phase centre corrections (PCC) variations are presented by analysing its component—the antennas’ phase centre offset (PCO). For this purpose, height differences were determined using different and independent methods: EUREF Permanent Network (EPN) combined solutions, Precise Point Positioning (PPP), and the single baseline solution. The results of GNSS processing were referenced to direct geometric levelling outputs. The research was conducted only within the global positioning system (GPS) system due to the compatibility of one of the receivers, and the experiment was based on a comparison of the height differences between four GNSS antennas located on the roof of a building: two permanent station antennas and two auxiliary points. The antennas were located at similar heights; precise height differences were determined by geometric levelling, both at the beginning and the end of the session. Post-processing was conducted with the use of the GPS system, precise ephemeris, the adopted antenna correction model, and a zero-elevation mask. For one of the antennas, a change of the antenna characteristic model from IGS08 to IGS14 leads to an 8-mm difference in height. Older antennas used in the national (or transnational) permanent network need individual PCC.
Investigating the Ability to Identify New Constructions in Urban Areas Using Images from Unmanned Aerial Vehicles, Google Earth, and Sentinel-2
Authors: Aliabad Fahime Arabi , Malamiri Hamid Reza Ghafarian , Shojaei Saeed , Sarsangi Alireza , Ferreira Carla Sofia Santos , Kalantari Zahra
Published: July 2022

One of the main problems in developing countries is unplanned urban growth and land use change. Timely identification of new constructions can be a good solution to mitigate some environmental and social problems. This study examined the possibility of identifying new constructions in urban areas using images from unmanned aerial vehicles (UAV), Google Earth and Sentinel-2. The accuracy of the land cover map obtained using these images was investigated using pixel-based processing methods (maximum likelihood, minimum distance, Mahalanobis, spectral angle mapping (SAM)) and object-based methods (Bayes, support vector machine (SVM), K-nearest-neighbor (KNN), decision tree, random forest). The use of DSM to increase the accuracy of classification of UAV images and the use of NDVI to identify vegetation in Sentinel-2 images were also investigated. The object-based KNN method was found to have the greatest accuracy in classifying UAV images (kappa coefficient = 0.93), and the use of DSM increased the classification accuracy by 4%. Evaluations of the accuracy of Google Earth images showed that KNN was also the best method for preparing a land cover map using these images (kappa coefficient = 0.83). The KNN and SVM methods showed the highest accuracy in preparing land cover maps using Sentinel-2 images (kappa coefficient = 0.87 and 0.85, respectively). The accuracy of classification was not increased when using NDVI due to the small percentage of vegetation cover in the study area. On examining the advantages and disadvantages of the different methods, a novel method for identifying new rural constructions was devised. This method uses only one UAV imaging per year to determine the exact position of urban areas with no constructions and then examines spectral changes in related Sentinel-2 pixels that might indicate new constructions in these areas. On-site observations confirmed the accuracy of this method.
MFST: Multi-Modal Feature Self-Adaptive Transformer for Infrared and Visible Image Fusion
Authors: Liu Xiangzeng , Gao Haojie , Miao Qiguang , Xi Yue , Ai Yunfeng , Gao Dingguo
Published: July 2022

Infrared and visible image fusion is to combine the information of thermal radiation and detailed texture from the two images into one informative fused image. Recently, deep learning methods have been widely applied in this task; however, those methods usually fuse multiple extracted features with the same fusion strategy, which ignores the differences in the representation of these features, resulting in the loss of information in the fusion process. To address this issue, we propose a novel method named multi-modal feature self-adaptive transformer (MFST) to preserve more significant information about the source images. Firstly, multi-modal features are extracted from the input images by a convolutional neural network (CNN). Then, these features are fused by the focal transformer blocks that can be trained through an adaptive fusion strategy according to the characteristics of different features. Finally, the fused features and saliency information of the infrared image are considered to obtain the fused image. The proposed fusion framework is evaluated on TNO, LLVIP, and FLIR datasets with various scenes. Experimental results demonstrate that our method outperforms several state-of-the-art methods in terms of subjective and objective evaluation.