bluuebunny/crossref_metadata_2025 huggingface dataset:
Adaptive Optics of Kyrieleis Plaques in Varicella Zoster Virus-Associated Posterior Uveitis: A Multimodal Imaging Analysis
Authors: Milella Paolo , Mapelli Chiara , Nassisi Marco , Leone Gaia , Ruggi Giada , Scialdone Antonio , Casalino Giuseppe , Viola Francesco
Published: January 2023

Kyrieleis plaques (KP) represent a peculiar type of vasculitis affecting retinal arterial branches in a beaded segmental pattern that can be found in several posterior inflammatory ocular conditions. The nature and precise location of KP is unclear. Adaptive Optics (AO) provides an in vivo visualization of retinal vasculature on a microscopic level, thus permitting a more detailed characterization of KP as compared to traditional imaging techniques. This study aims to report AO imaging of KP in Varicella Zoster virus (VZV)-associated posterior uveitis and to correlate the findings with traditional imaging techniques. Three patients diagnosed with VZV posterior uveitis underwent adaptive optics (AO) imaging and traditional multimodal imaging techniques, including fundus photography, fluorescein angiography, indocyanine green angiography and optical coherence tomography. In all subjects, AO imaging revealed segmental hyporeflectivity confined to the vessel wall, with no evidence of arterial wall disruption or extravascular involvement. In our series, AO findings support the view that KP are localized within the inner arterial wall, possibly at the endothelial level.
Effectiveness of a Primary Care Multidisciplinary Treatment for Patients with Chronic Pain Compared with Treatment as Usual
Authors: Bults Rinske M. , van Dongen Johanna M. , Ostelo Raymond W. J. G. , Nijs Jo , Keizer Doeke , van Wilgen C. Paul
Published: January 2023

To manage chronic pain, multidisciplinary interventions have been increasingly deployed, mostly in secondary or tertiary care settings. Evidence on the effectiveness of multidisciplinary intervention within primary care is scarce. This study examined the effectiveness of a primary care multidisciplinary treatment for chronic pain compared with treatment as usual (TAU). The intervention consisted of pain neuroscience education and treatment by a GP, psychologist, and physiotherapist. Both groups filled out patient-reported outcome measures at baseline, 6 months, and 12 months. The results indicated there were no statistically significant differences for the primary outcomes of pain intensity, number of pain sites, and health-related quality of life (HR-QoL). There was a statistically significant difference in the secondary outcome perceived health change in favor of the intervention group. None of the other differences were statistically significant. A post-hoc analysis showed that there were statistically significant effects on patients’ illness perceptions in favor of the intervention group. Based on the results, the findings do not support effectiveness of a low intensity outpatient multidisciplinary primary care treatment to treat chronic pain compared with TAU. However, as a result of several study limitations, it is considered unwarranted to conclude that multidisciplinary treatment in primary care is not valuable at all.
The Impact of Prior Antithrombotic Use on Blood Viscosity in Cardioembolic Stroke with Non-Valvular Atrial Fibrillation
Authors: Jung Yo-Han , Han Sang-Won , Park Joong-Hyun
Published: January 2023

Although clinical studies have demonstrated that prior use of antiplatelets was associated with decreased blood viscosity (BV) in patients with acute ischemic stroke, the impact of previous anticoagulant use on blood viscosity in cardioembolic stroke with non-valvular AF (NVAF) has not yet been clearly studied. This single-center retrospective observational study aimed to determine the impact of prior antithrombotic (antiplatelet and anticoagulant) use on BV in patients with cardioembolic stroke (CES) due to NVAF. Patients with CES and NVAF were analyzed with the following inclusion criteria: (1) patients over 20 years of age admitted within five days of stroke onset; (2) ischemic stroke presumably due to an NVAF-derived embolus; (3) compatible cortical/subcortical lesion on brain computed tomography or magnetic resonance imaging; (4) hemoglobin level of 10–18 mg/dL; and (5) receiving antiplatelets within five days or anticoagulants within two days if previously medicated. From the screening of 195 patients (22% of the total stroke population during the study period) who had experienced ischemic stroke with AF, 160 were included for the final analysis. Eighty-nine patients (56%) were taking antithrombotics (antiplatelet, 57%; warfarin, 13%; NOACs, 30%) regularly. Compared to patients without previous antithrombotic use, those with previous antithrombotic use (antiplatelets, warfarin, and NOACs) were significantly associated with decreased systolic BV (SBV) and diastolic BV (DBV) (p < 0.036). In multiple linear regression analysis, hematocrit (Hct) level and prior antithrombotic use were significantly associated with decreased SBV and DBV. Hct was positively correlated with increased SBV and DBV. In Hct-adjusted partial correlation analysis, prior uses of any antithrombotic agents were associated with decreased SBV (r < −0.270, p < 0.015) and DBV (r < −0.183, p < 0.044). In conclusion, this study showed that prior antithrombotic use (antiplatelets, VKAs, and NOACs) was associated with decreased SBV and DBV in patients presenting with acute CES secondary to NVAF. Our results indicated that previous use of NOACs may be a useful hemorheological parameter in patients with acute CES due to NVAF. Accumulation of clinical data from a large number of patients with the risk of stroke occurrence, initial stroke severity, and functional outcome is necessary to assess the usefulness of BV.
Circulating HHIP Levels in Women with Insulin Resistance and PCOS: Effects of Physical Activity, Cold Stimulation and Anti-Diabetic Drug Therapy
Authors: Zhou Xin , Wang Yanping , Chen Wenyun , Zhang Hongmin , He Yirui , Dai Han , Hu Wenjing , Li Ke , Zhang Lili , Chen Chen , Yang Gangyi , Li Ling
Published: January 2023

Serum human hedgehog-interacting protein (HHIP) concentration is associated with diabetes. However, the relationship between HHIP and polycystic ovary syndrome (PCOS) or abnormal sex hormones remains unknown. This study was an observational cross-sectional study, with additional short-term intervention studies and follow-up studies. Bioinformatics analysis was performed to explore the association of PCOS with metabolic-related genes and signaling pathways. OGTT and EHC were performed on all participants. Lipid infusion, cold exposure, and 45-min treadmill test were performed on all healthy women. A total of 137 women with PCOS were treated with metformin, GLP-1RA, or TZDs for 24 weeks. Serum HHIP levels were higher in insulin resistance (IR) and PCOS women. Circulating HHIP levels were significantly correlated with adiponectin (Adipoq) levels, obesity, IR, and metabolic indicators. A correlation presented between HHIP and DHEA-S, FAI, SHBG, and FSH. Serum HHIP levels were significantly elevated by oral glucose challenge in healthy women, but not affected by EHC. Lipid infusion decreased serum HHIP levels, while cold exposure increased HHIP levels in healthy women. GLP-1RA and TZD treatment reduced serum HHIP levels in PCOS women, while metformin treatment did not affect HHIP levels. HHIP may be a useful biomarker and novel drug target for PCOS and IR individuals.
GMRS Oncological Prosthesis with a Porous Coating Collar: A Good Option for Revision of Aseptic Loosening in the Lower Extremity
Authors: Xu Hairong , Li Yuan , Yu Feng , Liu Weifeng , Hao Lin , Zhang Qing , Niu Xiaohui
Published: January 2023

Background: Revisions for oncological prosthesis are especially challenging due to the limited bone quantity and poor quality that the patients still possess. The aims of this study were to ask (1) what is the cumulative survival of the Global Modular Replacement System (GMRS) prosthesis after revision? and (2) what are the long-term functional outcomes of these patients? Methods: We retrospectively reviewed 16 patients who developed aseptic loosening of a lower extremity prosthesis. There were nine males and seven females with a mean age of 28 years (range, 14–55 years). The 5-year and 8-year survivorship of the prosthesis were calculated. Function outcome was evaluated according to the score of the Musculoskeletal Tumor Society (MSTS). Results: At a mean of 90 months follow-up (range, 52–118 months), the cumulative survival of all revision prosthesis was 94% at both 5 and 8 years. There were two prosthesis failures including one infection and one repeated aseptic loosening. At the last follow-up, except for the infection case, 93.3% (14/15) of the patients did not develop repeated aseptic loosening. The mean MSTS score was 27.7 (range, 24–30). Conclusions: GMRS prosthesis demonstrated significant satisfactory long-term outcomes for revisions of lower extremity oncological prosthesis.
Temperature-Dependent Deformation Behavior of “γ-austenite/δ-ferrite” Composite Obtained through Electron Beam Additive Manufacturing with Austenitic Stainless-Steel Wire
Authors: Astafurova Elena , Maier Galina , Melnikov Evgenii , Astafurov Sergey , Panchenko Marina , Reunova Kseniya , Luchin Andrey , Kolubaev Evgenii
Published: January 2023

Temperature dependence of tensile deformation behavior and mechanical properties (yield strength, ultimate tensile strength, and an elongation-to-failure) of the dual-phase (γ-austenite/δ-ferrite) specimens, obtained through electron-beam additive manufacturing, has been explored for the first time in a wide temperature range T = (77–300) K. The dual-phase structures with a dendritic morphology of δ-ferrite (γ + 14%δ) and with a coarse globular δ-phase (γ + 6%δ) are typical of the as-built specimens and those subjected to a post-production solid–solution treatment, respectively. In material with lower δ-ferrite content, the lower values of the yield strength in the whole temperature range and the higher elongation of the specimens at T > 250 K have been revealed. Tensile strength and stages of plastic flow of the materials do not depend on the δ-ferrite fraction and its morphology, but the characteristics of strain-induced γ→α′ and γ→ε→α′ martensitic transformations and strain-hardening values are different for two types of the specimens. A new approach has been applied for the analysis of deformation behavior of additively fabricated Cr-Ni steels. Mechanical properties and plastic deformation of the dual-phase (γ + δ) steels produced through electron beam additive manufacturing have been described from the point of view of composite materials. Both types of the δ-ferrite inclusions, dendritic lamellae and globular coarse particles, change the stress distribution in the bulk of the materials during tensile testing, assist the defect accumulation and partially suppress strain-induced martensitic transformation.
Water Temperature Prediction Using Improved Deep Learning Methods through Reptile Search Algorithm and Weighted Mean of Vectors Optimizer
Authors: Ikram Rana Muhammad Adnan , Mostafa Reham R. , Chen Zhihuan , Parmar Kulwinder Singh , Kisi Ozgur , Zounemat-Kermani Mohammad
Published: January 2023

Precise estimation of water temperature plays a key role in environmental impact assessment, aquatic ecosystems’ management and water resources planning and management. In the current study, convolutional neural networks (CNN) and long short-term memory (LSTM) network-based deep learning models were examined to estimate daily water temperatures of the Bailong River in China. Two novel optimization algorithms, namely the reptile search algorithm (RSA) and weighted mean of vectors optimizer (INFO), were integrated with both deep learning models to enhance their prediction performance. To evaluate the prediction accuracy of the implemented models, four statistical indicators, i.e., the root mean square errors (RMSE), mean absolute errors, determination coefficient and Nash–Sutcliffe efficiency were utilized on the basis of different input combinations involving air temperature, streamflow, precipitation, sediment flows and day of the year (DOY) parameters. It was found that the LSTM-INFO model with DOY input outperformed the other competing models by considerably reducing the errors of RMSE and MAE in predicting daily water temperature.
Isolation of Main Pathogens Causing Postharvest Disease in Fresh Codonopsis pilosula during Different Storage Stages and Ozone Control against Disease and Mycotoxin Accumulation
Authors: Lv Bingyu , Yang Xi , Xue Huali , Nan Mina , Zhang Yuan , Liu Zhiguang , Bi Yang , Shang Suqin
Published: January 2023

Codonopsis pilosula is an important Chinese herbal medicine. However, fresh C. pilosula is prone to decay during storage due to microorganism infections, seriously affecting the medicinal value and even causing mycotoxin accumulation. Therefore, it is necessary to study the pathogens present and develop efficient control strategies to mitigate their detrimental effects on the herbs during storage. In this study, fresh C. pilosula was collected from Min County in Gansu Province, China. The natural disease symptoms were observed during different storage stages, and the pathogens causing C. pilosula postharvest decay were isolated from the infected fresh C. pilosula. Morphological and molecular identification were performed, and pathogenicity was tested using Koch’s postulates. In addition, the control of ozone was examined against the isolates and mycotoxin accumulation. The results indicated that the naturally occurring symptom increased progressively with the extension of storage time. The mucor rot caused by Mucor was first observed on day 7, followed by root rot caused by Fusarium on day 14. Blue mold disease caused by Penicillum expansum was detected as the most serious postharvest disease on day 28. Pink rot disease caused by Trichothecium roseum was observed on day 56. Moreover, ozone treatment significantly decreased the development of postharvest disease and inhibited the accumulations of patulin, deoxynivalenol, 15-Acetyl-deoxynivalenol, and HT-2 toxin.
Inborn Errors of Immunity Causing Pediatric Susceptibility to Fungal Diseases
Authors: Olbrich Peter , Vinh Donald C.
Published: January 2023

Inborn errors of immunity are a heterogeneous group of genetically determined disorders that compromise the immune system, predisposing patients to infections, autoinflammatory/autoimmunity syndromes, atopy/allergies, lymphoproliferative disorders, and/or malignancies. An emerging manifestation is susceptibility to fungal disease, caused by yeasts or moulds, in a superficial or invasive fashion. In this review, we describe recent advances in the field of inborn errors of immunity associated with increased susceptibility to fungal disease.
Four New Species of Torula (Torulaceae, Pleosporales) from Sichuan, China
Authors: Tian Wenhui , Su Pengwei , Chen Yanpeng , Maharachchikumbura Sajeewa S. N.
Published: January 2023

Torula is an asexual and hyphomycetous genus in the family Torulaceae. Torula species are generally saprophytic. They have a worldwide distribution and abound in humid or freshwater habitats. In order to better understand this genus, we carried out several field collections from Sichuan, China. As a result, we obtained nine Torula isolates from dead woody substrates in terrestrial and freshwater habitats. Based on a biphasic approach of morphological examination and multi-locus phylogenetic analyses (ITS, SSU, LSU, TEF, RPB2), these collections were identified as belonging to seven Torula species. Four of them were new species (Torula chinensis, T. longiconidiophora, T. sichuanensis and T. submersa), and the other three belonged to existing species, though one was found for the first time in China (T. masonii). Morphological and updated phylogenetic delamination of the new discoveries is also discussed. This study provides further insights into our understanding of wood-based Torula species in China.
Data-Driven Deep Journalism to Discover Age Dynamics in Multi-Generational Labour Markets from LinkedIn Media
Authors: Alaql Abeer Abdullah , AlQurashi Fahad , Mehmood Rashid
Published: January 2023

We live in the information age and, ironically, meeting the core function of journalism—i.e., to provide people with access to unbiased information—has never been more difficult. This paper explores deep journalism, our data-driven Artificial Intelligence (AI) based journalism approach to study how the LinkedIn media could be useful for journalism. Specifically, we apply our deep journalism approach to LinkedIn to automatically extract and analyse big data to provide the public with information about labour markets; people’s skills and education; and businesses and industries from multi-generational perspectives. The Great Resignation and Quiet Quitting phenomena coupled with rapidly changing generational attitudes are bringing unprecedented and uncertain changes to labour markets and our economies and societies, and hence the need for journalistic investigations into these topics is highly significant. We combine big data and machine learning to create a whole machine learning pipeline and a software tool for journalism that allows discovering parameters for age dynamics in labour markets using LinkedIn data. We collect a total of 57,000 posts from LinkedIn and use it to discover 15 parameters by Latent Dirichlet Allocation algorithm (LDA) and group them into 5 macro-parameters, namely Generations-Specific Issues, Skills and Qualifications, Employment Sectors, Consumer Industries, and Employment Issues. The journalism approach used in this paper can automatically discover and make objective, cross-sectional, and multi-perspective information available to all. It can bring rigour to journalism by making it easy to generate information using machine learning, and can make tools and information available so that anyone can uncover information about matters of public importance. This work is novel since no earlier work has reported such an approach and tool and leveraged it to use LinkedIn media for journalism and to discover multigenerational perspectives (parameters) for age dynamics in labour markets. The approach could be extended with additional AI tools and other media.
Jet Ventilation Reduces Coronary Sinus Movement in Patients Undergoing Atrial Fibrillation Ablation: An Observational Crossover Study
Authors: Maeyens Cécile , Nokerman Pierre , Casado-Arroyo Ruben , Abugattas De Torres Juan-Pablo , Alexander Brenton , Engelman Edgard , Schmartz Denis , Tuna Turgay
Published: January 2023

Background: One of the reasons that high-frequency jet ventilation (HFJV) is used is due to the near immobility of thoracic structures. However, no study has quantified the movements of cardiac structures during HFJV compared with normal mechanical ventilation. Methods: After ethical approval and written informed consent, we included 21 patients scheduled for atrial fibrillation ablation in this prospective crossover study. Each patient was ventilated with both normal mechanical ventilation and HFJV. During each ventilation mode, displacements of the cardiac structure were measured by the EnSite Precision mapping system using a catheter placed in the coronary sinus. Results: The median [Q1–Q4] displacement was 2.0 [0.6–2.8] mm during HFJV and 10.5 [9.3–13.0] mm during conventional ventilation (p < 0.000001). Conclusion: This study quantifies the minimal movement of cardiac structures during HFJV compared to standard mechanical ventilation.
Management of Irreparable Posterosuperior Rotator Cuff Tears—A Current Concepts Review and Proposed Treatment Algorithm by the AGA Shoulder Committee
Authors: Pogorzelski Jonas , Rupp Marco-Christopher , Scheiderer Bastian , Lacheta Lucca , Schliemann Benedikt , Schanda Jakob , Heuberer Philipp , Schneider Marco , Hackl Michael ,   AGA Shoulder Committee—Rotator Cuff, Lorbach Olaf
Published: January 2023

Posterosuperior rotator cuff tears range among the most common causes of shoulder complaints. While non-operative treatment is typically reserved for the elderly patient with low functional demands, surgical treatment is considered the gold standard for active patients. More precisely, an anatomic rotator cuff repair (RCR) is considered the most desirable treatment option and should be generally attempted during surgery. If an anatomic RCR is impossible, the adequate choice of treatment for irreparable rotator cuff tears remains a matter of debate among shoulder surgeons. Following a critical review of the contemporary literature, the authors suggest the following evidence- and experience-based treatment recommendation. In the non-functional, osteoarthritic shoulder, treatment strategies in the management of irreparable posterosuperior RCT include debridement-based procedures and reverse total shoulder arthroplasty as the treatment of choice. Joint-preserving procedures aimed at restoring glenohumeral biomechanics and function should be reserved for the non-osteoarthritic shoulder. Prior to these procedures, however, patients should be counseled about deteriorating results over time. Recent innovations such as the superior capsule reconstruction and the implantation of a subacromial spacer show promising short-term results, yet future studies with long-term follow-up are required to derive stronger recommendations.
Laboratory Findings and Clinical Outcomes of ICU-admitted COVID-19 Patients: A Retrospective Assessment of Particularities Identified among Romanian Minorities
Authors: Mocanu Alexandra , Lazureanu Voichita Elena , Laza Ruxandra , Marinescu Adelina Raluca , Cut Talida Georgiana , Sincaru Suzana-Vasilica , Marza Adina Maria , Popescu Irina-Maria , Herlo Lucian-Flavius , Nelson-Twakor Andreea , Rivis Mircea , Bratosinand Felix , Porosnicu Tamara Mirela , Mederle Alexandru Ovidiu
Published: January 2023

The Roma population accounts for over 3% (approximately 10 to 15 million) of Romania’s permanent population, and it represents one of Europe’s most impoverished populations. Due to poverty and unemployment, Romania’s Roma minority may have diminished access to healthcare and preventive medicine. The limited existing evidence suggests that the European Roma group has been at a higher risk of becoming ill and dying during the pandemic owing to their lifestyle choices, socioeconomic circumstances, and genetic pathophysiological traits. As a result, the purpose of the present research was to investigate the link between the inflammatory markers implicated and the clinical progression of COVID-19 in Roma patients who were brought to the intensive care unit. We considered 71 Roma patients admitted to the ICU with SARS-CoV-2 infection and 213 controls from the general population with the same inclusion criteria. The body mass index of patients was statistically significantly higher among Roma patients, with more than 57% being overweight, compared with 40.7% in the control group. Frequent smoking was more prevalent in patients of Roma ethnicity admitted to the ICU and the number of comorbidities. We observed a significantly higher proportion of severe imaging features at admission in the group of cases, although this difference may have been associated with the higher prevalence of smoking in this group. The mean duration of hospitalization was longer by 1.8 days than the control group. Elevated ESR levels were observed in 54.0% of Roma patients at admission, compared with 38.9% in the control group. Similarly, 47.6% of them had elevated CRP levels. IL-6 increased significantly at the time of ICU admission, similarly to the significant rise in the CRP levels, compared with the general population. However, the proportion of intubated patients and mortality did not differ significantly. On multivariate analysis, the Roma ethnicity significantly influenced the CRP (β = 1.93, p-value = 0.020) and IL-6 (β = 1.85, p-value = 0.044). It is necessary to plan different healthcare strategies aimed at special populations, such as the Roma ethnicity, to prevent the reduced disparities presented in in this study.
Comparisons of Mechanical Power and Respiratory Mechanics in Pressure-Controlled Ventilation and Volume-Controlled Ventilation during Laparoscopic Cholecystectomy in Elderly Patients
Authors: Jo Youn Yi , Chang Young Jin , Lee Dongchul , Kim Yong Beom , Jung Junsu , Kwak Hyun Jeong
Published: January 2023

We compared the effects of pressure-controlled volume-guaranteed ventilation (PCV) and volume-controlled ventilation (VCV) on respiratory mechanics and mechanical power (MP) in elderly patients undergoing laparoscopy. Fifty patients aged 65–80 years scheduled for laparoscopic cholecystectomy were randomly assigned to either the VCV group (n = 25) or the PCV group (n = 25). The ventilator had the same settings in both modes. The change in MP over time was insignificant between the groups (p = 0.911). MP significantly increased during pneumoperitoneum in both groups compared with anesthesia induction (IND). The increase in MP from IND to 30 min after pneumoperitoneum (PP30) was not different between the VCV and PCV groups. The change in driving pressure (DP) over time were significantly different between the groups during surgery, and the increase in DP from IND to PP30 was significantly higher in the VCV group than in the PCV group (both p = 0.001). Changes in MP during PCV and VCV were similar in elderly patients, and MP increased significantly during pneumoperitoneum in both groups. However, MP did not reach clinical significance (≥12 J/min). In contrast, the PCV group had a significantly lower increase in DP after pneumoperitoneum than the VCV group.
Writing Development and Translanguaging in Signing Bilingual Deaf Children of Deaf Parents
Authors: Holcomb Leala
Published: January 2023

Translanguaging allows for a holistic lens on the natural language practices of multilinguals and the strategies they use to communicate and to make meaning, such as writing. Multilinguals have a single, integrated linguistic repertoire and reasoning in which all languages are naturally activated in the brain throughout the writing process. Some deaf children are raised in environments where signed language is first acquired and then used to support the development of a written language. The current literature on translanguaging is scant in capturing signing bilingual deaf writers’ translanguaging practices. This study took a closer look at the written texts produced by three deaf siblings within an American Sign Language/English bilingual deaf family across the course of their childhood between three and ten years old. Their applications of linguistic features in writing over time were qualitatively identified, described, and analyzed. This study’s findings provided rich descriptions and examples of the ways signing bilingual deaf children leverage their whole linguistic repertoire to express ideas in print. Because translanguaging practices were modeled at home and school during natural interactions and direct instruction, these siblings’ language and writing skills continued to flourish over time.
Dissipative Particle Dynamics Simulations for Shape Change of Growing Lipid Bilayer Vesicles
Authors: Mitsuhashi Hiromi , Morikawa Ryota , Noguchi Yoh , Takasu Masako
Published: January 2023

The characteristic shape changes observed in the growth and division of L-form cells have been explained by several theoretical studies and simulations using a vesicle model in which the membrane area increases with time. In those theoretical studies, characteristic shapes such as tubulation and budding were reproduced in a non-equilibrium state, but it was not possible to incorporate deformations that would change the topology of the membrane. We constructed a vesicle model in which the area of the membrane increases using coarse-grained particles and analyzed the changes in the shape of growing membrane by the dissipative particle dynamics (DPD) method. In the simulation, lipid molecules were added to the lipid membrane at regular time intervals to increase the surface area of the lipid membrane. As a result, it was found that the vesicle deformed into a tubular shape or a budding shape depending on the conditions for adding lipid molecules. This suggests that the difference in the place where new lipid molecules are incorporated into the cell membrane during the growth of L-form cells causes the difference in the transformation pathway of L-form cells.
Green Biomass-Based Protein for Sustainable Feed and Food Supply: An Overview of Current and Future Prospective
Authors: Domokos-Szabolcsy Éva , Yavuz Seckin Reyhan , Picoli Edgard , Fári Miklós Gabor , Kovács Zoltán , Tóth Csaba , Kaszás László , Alshaal Tarek , Elhawat Nevien
Published: January 2023

It is necessary to develop and deploy novel protein production to allow the establishment of a sustainable supply for both humans and animals, given the ongoing expansion of protein demand to meet the future needs of the increased world population and high living standards. In addition to plant seeds, green biomass from dedicated crops or green agricultural waste is also available as an alternative source to fulfill the protein and nutrient needs of humans and animals. The development of extraction and precipitation methods (such as microwave coagulation) for chloroplast and cytoplasmic proteins, which constitute the bulk of leaf protein, will allow the production of leaf protein concentrates (LPC) and protein isolates (LPI). Obtained LPC serves as a sustainable alternative source of animal-based protein besides being an important source of many vital phytochemicals, including vitamins and substances with nutritional and pharmacological effects. Along with it, the production of LPC, directly or indirectly, supports sustainability and circular economy concepts. However, the quantity and quality of LPC largely depend on several factors, including plant species, extraction and precipitation techniques, harvest time, and growing season. This paper provides an overview of the history of green biomass-derived protein from the early green fodder mill concept by Károly Ereky to the state-of-art of green-based protein utilization. It highlights potential approaches for enhancing LPC production, including dedicated plant species, associated extraction methods, selection of optimal technologies, and best combination approaches for improving leaf protein isolation.
Using the Gut Microbiome to Assess Stocking Efforts of the Endangered Pallid Sturgeon, Scaphirhynchus albus
Authors: Gaughan Sarah , Kyndt John A. , Haas Justin D. , Steffensen Kirk D. , Kočovský Patrick M. , Pope Kevin L.
Published: January 2023

The endangered Pallid Sturgeon, Scaphirhynchus albus, has been actively managed to prevent population declines, including stocking of hatchery-raised fish. The gut microbiome plays an innate role in an organism’s absorption of nutrients by increasing nutrient availability and can provide new insights for Pallid Sturgeon management. In this study, the Pallid Sturgeon’s microbiome is dominated by the phyla Proteobacteria, Firmicutes, Actinobacteria and Fusobacteria. It was also determined that the gut bacterial diversity in hatchery-raised Pallid Sturgeon was not significantly different from wild Pallid Sturgeon, supporting that hatchery-raised Pallid Sturgeon are transitioning effectively to wild diets. There is also a high degree of intraspecific variation in the bacterial and eukaryotic sequences amongst individual Pallid Sturgeon microbiomes, suggesting the Pallid Sturgeon may be omnivorous. This study demonstrated that genetic markers may be used to effectively describe the dietary requirements for wild Pallid Sturgeon and provides the first genetic evidence that Pallid Sturgeons are effectively transitioning from hatchery-raised environments to the wild.
Relationship between Nutrition, Lifestyle Habits and Laboratory Parameters in Hypertensive Patients with/without Cognitive Dysfunction
Authors: Nyulas Kinga-Ilona , Germán-Salló Márta , Fazakas Zita , Preg Zoltán , Pál Tünde , Pál Sándor , Tripon Robert Gabriel , Cseh Margit Judit , Simon-Szabó Zsuzsánna , Arbănași Emil Marian , Nemes-Nagy Enikő
Published: January 2023

(1) Background: Cognitive dysfunction is a major concern in hypertensive patients. Lifestyle habits and nutrition influence laboratory parameters, with an impact on clinical course. The objective of the study was to evaluate nutrition and lifestyle habits in hypertensive patients with/without cognitive dysfunction and establish correlations to laboratory parameters. Material and Methods: 50 patients admitted to the Cardiovascular Rehabilitation Clinic in Târgu Mureș were enrolled in this study between March–June 2021. We evaluated their cognitive function, and they filled in a questionnaire about lifestyle and nutrition. Biochemical blood tests were performed using a Konelab Prime 60i analyzer. IBM-SPSS22 and GraphPad InStat3 were used for statistics. Results: Mean age of hypertensive patients (n = 50) was 70.42 ± 4.82 (SD) years, half of them had cognitive dysfunction. Zinc deficiency was present in 74% of the subjects. The subgroup with cognitive dysfunction had significantly higher BMI (p = 0.009) and microalbuminuria (p = 0.0479), as well as significantly lower magnesium intake (p = 0.032) and cholesterol intake (p = 0.022), compared to those with normal cognitive status. Conclusions: Nutrition is in a close relationship with laboratory parameters; significant differences (microalbuminuria, cholesterol intake, BMI, etc.) are present between hypertensive patients with/without cognitive dysfunction. A healthy diet is important for the maintenance of metabolic balance, the achievement of optimal body weight, and the prevention of complications.
Physiological and Biochemical Responses in Microalgae Dunaliella salina, Cylindrotheca closterium and Phormidium versicolor NCC466 Exposed to High Salinity and Irradiation
Authors: Guermazi Wassim , Masmoudi Salma , Trabelsi Neila Annabi , Gammoudi Sana , Ayadi Habib , Morant-Manceau Annick , Hotos George N.
Published: January 2023

Dunaliella salina (Chlorophyceae), Phormidium versicolor (Cyanophyceae), and Cylindrotheca closterium (Bacillariophyceae) were isolated from three ponds in the solar saltern of Sfax (Tunisia). Growth, pigment contents, and photosynthetic and antioxidant enzyme activities were measured under controlled conditions of three light levels (300, 500, and 1000 µmol photons m−2 s−1) and three NaCl concentrations (40, 80, and 140 g L−1). The highest salinity reduced the growth of D. salina and P. versicolor NCC466 and strongly inhibited that of C. closterium. According to ΦPSII values, the photosynthetic apparatus of P. versicolor was stimulated by increasing salinity, whereas that of D. salina and C. closterium was decreased by irradiance rise. The production of carotenoids in D. salina and P. versicolor was stimulated when salinity and irradiance increased, whereas it decreased in the diatom. Catalase (CAT), Superoxide dismutase (SOD), and Ascorbate peroxidase (APX) activities were only detected when the three species were cultivated under E1000. The antioxidant activity of carotenoids could compensate for the low antioxidant enzyme activity measured in D. salina. Salinity and irradiation levels interact with the physiology of three species that have mechanisms of more or less effective stress resistance, hence different resistance to environmental stresses according to the species. Under these stress-controlled conditions, P. versicolor and C. closterium strains could provide promising sources of extremolyte for several purposes.
Incoherent Neutron Scattering and Terahertz Time-Domain Spectroscopy on Protein and Hydration Water
Authors: Nakagawa Hiroshi , Yamamoto Naoki
Published: January 2023

Incoherent inelastic and quasi-elastic neutron scattering (INS) and terahertz time-domain spectroscopy (THz-TDS) are spectroscopy methods that directly detect molecular dynamics, with an overlap in the measured energy regions of each method. Due to the different characteristics of their probes (i.e., neutron and light), the information obtained and the sample conditions suitable for each method differ. In this review, we introduce the differences in the quantum beam properties of the two methods and their associated advantages and disadvantages in molecular spectroscopy. Neutrons are scattered via interaction with nuclei; one characteristic of neutron scattering is a large incoherent scattering cross-section of a hydrogen atom. INS records the auto-correlation functions of atomic positions. By using the difference in neutron scattering cross-sections of isotopes in multi-component systems, some molecules can be selectively observed. In contrast, THz-TDS observes the cross-correlation function of dipole moments. In water-containing biomolecular samples, the absorption of water molecules is particularly large. While INS requires large-scale experimental facilities, such as accelerators and nuclear reactors, THz-TDS can be performed at the laboratory level. In the analysis of water molecule dynamics, INS is primarily sensitive to translational diffusion motion, while THz-TDS observes rotational motion in the spectrum. The two techniques are complementary in many respects, and a combination of the two is very useful in analyzing the dynamics of biomolecules and hydration water.
Modeling of Interfacial Tension and Inclusion Motion Behavior in Steelmaking Continuous Casting Mold
Authors: Siddiqui Md Irfanul Haque , Arifudin Latif , Alnaser Ibrahim Abdullah , Ali Masood Ashraf , Alluhydan Khalid
Published: January 2023

The current work is an expansion of our previous numerical model in which we investigated the motion behavior of mold inclusions in the presence of interfacial tension effects. In this paper, we used computational fluid dynamic simulations to examine the influence of interfacial tension on inclusion motion behavior near to the solid–liquid interface (solidifying shell). We have used a multiphase model in which molten steel (SPFH590), sulfur, and alumina inclusions have been considered as different phases. In addition, we assume minimal to negligible velocity at the solid–liquid interface, and we restrict the numerical simulation to only include critical phenomena like heat transport and interfacial tension distribution in two-dimensional space. The two-phase simulation of molten steel mixed with sulfur and alumina was modeled on volume of fluid (VOF) method. Furthermore, the concentration of the surfactant (sulfur) in molten steel was defined using a species model. The surfactant concentration and temperature affect the Marangoni forces, and subsequently affects the interfacial tension applied on inclusion particles. It was found that the alteration in interfacial tension causes the inclusion particles to be pushed and swallowed near the solidifying boundaries. In addition, we have compared the computational results of interfacial tension, and it was found to be in good agreement with experimental correlations.
Dielectric Spectroscopy of Calcium Titanate Processed by Spark Plasma Sintering
Authors: Ctibor Pavel , Sedláček Josef , Straka Libor , Lukáč František , Neufuss Karel
Published: January 2023

Calcium titanate (CaTiO3) powder was compacted by spark plasma sintering (SPS). The resulting products were subjected to the phase stability study and dielectric characterization. The change in temperature of SPS between 1100 °C and 1250 °C had a clear and straightforward effect on density, porosity, relative permittivity, loss tangent, and DC resistivity. Since the SPS itself introduces certain oxygen deficiency into Ti-perovskites, all samples were annealed after SPS. However, this post-processing did not mask the effects of the SPS regime. Optical reflectance measurements were completed to compare and quantify the sample coloration and support the dielectric results with corresponding optical band gap estimations. Subtle changes in the CaTiO3 crystal lattice arrangement, completed between 1150 °C and 1250 °C and documented in the literature for conventionally sintered samples, could not be confirmed for SPS-prepared calcium titanate. The novelty of this research work is in producing very stable dielectric ceramics and an indication of the SPS processing parameters suitable for this. The best sample showed at 1 MHz frequency the combination of relative permittivity 370, loss tangent 0.008, and DC resistivity 3 × 1012 Ωm.
Novel Nanocomposite Hydrogels Based on Crosslinked Microbial Polysaccharide as Potential Bioactive Wound Dressings
Authors: Marin Maria Minodora , Albu Kaya Madalina , Kaya Durmus Alpaslan , Constantinescu Roxana , Trica Bogdan , Gifu Ioana Catalina , Alexandrescu Elvira , Nistor Cristina Lavinia , Alexa Rebeca Leu , Ianchis Raluca
Published: January 2023

A multitude of dressings have been developed to promote wound repair, such as membranes, foams, hydrocolloids and hydrogels. In this study, a crosslinked polysaccharide hydrogel was mixed with a bioactive ingredient to synthesize a novel nanocomposite material to be used in wound healing. Variation of the ratio between hydrogel components was followed and its effect was analyzed in regard to swelling, degradation rate and thermo-mechanical behavior. The resulting crosslinked structures were characterized by FTIR and microscopy analyses. The antimicrobial activity of the crosslinked hydrogels loaded with bioactive agent was evaluated using two bacterial strains (Gram-positive Staphylococcus aureus and Gram-negative bacteria Escherichia Coli). All the results showed that the new synthesized biopolymer nanocomposites have adequate properties to be used as antibacterial wound dressings.
Acknowledgment to the Reviewers of Materials in 2022
Authors:   Materials Editorial Office
Published: January 2023

High-quality academic publishing is built on rigorous peer review [...]
An SMA Transducer for Sensing Tactile Sensation Focusing on Stroking Motion
Authors: Oya Ryusei , Sawada Hideyuki
Published: January 2023

The authors have developed a micro-vibration actuator using filiform SMA wire electrically driven by periodic electric current. While applying the SMA actuators to tactile displays, we discovered a phenomenon that the deformation caused by a given stress to an SMA wire generated a change in the electrical resistance. With this characteristic, the SMA wire works as a micro-force sensor with high sensitivity, while generating micro-vibration. In this paper, the micro-force sensing ability of an SMA transducer is described and discussed. Experiments are conducted by sliding the SMA sensor on the surface of different objects with different speeds, and the sensing ability is evaluated to be related with human tactile sensation.
Assessment of Residual Useful Life of Sun Gear in a Planetary Gearbox Based on Dynamic Wear Behaviors Analyses
Authors: Wang Jian , Zhang Jun
Published: January 2023

For proactively arranged downtime and maintenance of the gearbox, the accurate assessment of residual useful life (RUL) for worn-surface gear is a critical problem that needs to be solved. In light of this, a novel dynamic assessment methodology for RUL of sun gear was proposed by coupling Archard’s wear model and a nonlinear dynamics model. By modifying the internal excitation model, the effect of tooth surface wear is included in the dynamics model. Then, the effect of dynamic behavior on the wear process of the tooth surface is further fed back by the dynamic meshing force. On this basis, the influence of input speed, torque load and initial wear depth on the RUL was analyzed using a planetary gear NGW11-6 as an example. The results indicate that the torque load has the greatest influence on the RUL, followed by the initial wear depth, and that the input speed has the least influence. The established methodology will provide a theoretical basis for the RUL assessment and scheduled maintenance of transmission systems with worn-surface gears.
Optimal Design and Dynamic Analysis of a Spring-Actuated Cam-Linkage Mechanism in a Vacuum Circuit Breaker
Authors: Kang Yaw-Hong , Huang Hua-Chih , Yang Bo-Ya
Published: January 2023

This study attempted to establish an optimal design and perform dynamic analysis for a spring-actuated cam-linkage composite mechanism in a rated 12 kV, 25 kA vacuum circuit breaker (VCB). The optimal design of the VCB mechanism involves two steps: the first step involves the optimal design of the stiffness of closing springs and the cam profile, based on three-order polynomial motion curve of the follower; the second step involves the optimal design of a four-bar linkage. To minimize the surplus work in the VCB operating mechanism, the overall difference between the general output force and general resistant force of the VCB operating mechanism during the closing operation was adopted as the objective function to be minimized. Furthermore, the differential evolution algorithm with the golden ratio (DE-gr), an efficient and accuracy metaheuristic algorithm, was employed as the optimization method. The dynamic analysis of the optimal operating mechanism was conducted using a MATLAB-coded program and verified by the multibody dynamic software MSC-ADAMS. The dynamic analysis revealed that the optimal design of the VCB mechanism can considerably decrease the average closing velocity of the movable contact and the Hertz stress between the contact surface of the cam and the roller follower.
Model-Free Output-Feedback Sliding-Mode Control Design for Piezo-Actuated Stage
Authors: Yeh Yi-Liang , Pan Hsuan-Wei , Shen Yuan-Hong
Published: January 2023

Hysteresis in a piezoelectric actuator must be compensated for, and this compensation constitutes the main challenge in the high-precision motion control of piezo-actuated stages. This paper presents an output-feedback sliding-mode control (SMC) scheme to suppress unknown nonlinearity; in this scheme, hysteresis behavior is considered an external disturbance, and complex hysteresis models are thus not required. The scheme functions in the absence of transfer function of system state information, and a robust loop-transfer recovery observer is employed as a noise-free differentiator to estimate the required signal derivatives when the relevant system is in a noisy environment.
Investigation of Flow and Heat Transfer Characteristics in Microchannels with Fins
Authors: Li Murun , Gao Xuan , Li Haiwang , Sang Jichang , Nie Pengpeng , Fang Weidong , Xu Tiantong
Published: January 2023

A highly efficient thermal management is imperative to overcome the main challenges associated with heat extraction requirements in electronics. In this study, the flow and heat transfer characteristics of microchannels with various types of fins were numerically analyzed for Re = 0–500 (Re: Reynolds number). Investigation of the aspect ratio, incident angle, and smoothness as well as the flow and heat transfer behaviors revealed the exceptional performance of the optimized fin structure, up to a performance evaluation criterion of 1.53. At large Re values, the fin with a high aspect ratio, small incidence angle, and high smoothness showed the best performance, as it avoids stagnation zones because of flares and sharp corners and simultaneously leads to boundary layer destruction and redevelopment. Interestingly, the microchannel without internal microstructures performed well at small Re values. Among all the designed variables, the influence of the incident angle was superior owing to its ability to generate significant vortices by periodically changing the channel cross-sectional area and flow direction. The conclusions can be innovatively generalized to other microchannels with fins.