GC-MS Characterization of Bioactive Compounds from the Whole Plants Analysis of Ethyl Acetate Fractions of Talinum fruticosum(Water Leaf)
Authors: Ojoago Abba Benedict , V Sheneni 
Published: January 2025

Plants have played an important role in the fight for survival from man’s evolution. Plants aren’t just the principal food source
for all animals and even humans, but there are a lot of plant species that have been shown to be very valuable medicinal
products over time. Medicinal plants are used to treat and diagnose and infections from ancients times, plants have been
rich source of effective and safe medicines.While Ethiopia has a long history of a traditional health care system, research on
traditional medicinal plants (TMP) has lagged behind the country's multiethnic, cultural, and flora diversity (Fenta-country's
multiethnic, cultural, and flora diversity). Nevertheless, medicinal plants continue to play significant roles in the daily lives
of people living in developing countries in Asia and Africa, including Ethiopia.cines . According to estimates from the World
Health Organization (WHO), between 75 and 80 percent of the world's population already uses herbal medicine for some
aspect of primary healthcare . Plants with a long history of usage in ethnomedicine offer a valuable source of medicines for the
treatment of many illnesses and infectious diseases in pharmaceutical conditions. Most herbal medicines and their derivative
products were often prepared from crude plant extracts, which comprise a complex mixture of different phytochemical
constituents (plant secondary metabolites). The aim of the research was to characterized the bioactive compounds of ethyl
acetate fraction using gas-chromatography mass spectrophotometer.The resultant fraction was analyzed using GC-MS. Fatty
acids, sterols, and terpenoids were among the bioactive substances found in the sample, according to the analysis. Different
chemicals were indicated by the unique peaks in the GC-MS chromatogram. Hexadecanoic acid (palmitic acid), phytol, 2-decanol,
2-methyl-3-(3-methyl-but-2-enyl)-2-(4-methyl-pent-3-enyl), cyclotrisiloxanehexamethyl, pentanedioic acid, 2-oxo-, and
dimethyl ester are among the main chemicals found. Numerous pharmacological characteristics, including anti-inflammatory,
antioxidant, and antibacterial effects, have been documented for these substances. To assess the distinct biological activity
and possible therapeutic uses of the purified compounds, in vitro and in vivo investigations had to be conducted. Talinum
fruticosum (water leaf) are used for the treatments of various ailments in folklore medicine, Waterleaf also holds medicinal
significance. It is believed to possess various health benefits, including anti-inflammatory, diuretic, and antioxidant properties.
Traditional medicine practitioners often use water leaf to treat ailments such as gastrointestinal disorders, malaria, and high
blood pressure. and high blood pressure. Bioactive compounds, which are bioactive compounds found in plants, have gained
significant attention due to their potential health benefits and therapeutic properties. These compounds include alkaloids,
flavonoids, tannins, saponins, and phenolic compounds, among others . The extraction of these bioactive compounds is crucial
for their characterization and subsequent application in various industries, including pharmaceuticals, nutraceuticals, and
cosmetics.