bluuebunny/crossref_metadata_2025 huggingface dataset:
A longitudinal examination of language learners’ group-level enjoyment and emotion regulation in online collaborative learning
Authors: Zhang Zhipeng , Liu Ting , Gao Xuesong (Andy) , Lee Chwee Beng
Published: January 2024

This study examines the evolution and regulation of group-level enjoyment of online collaborative language learning. Three Chinese undergraduate learners of English as a foreign language (EFL) collaborated to finish a series of English writing tasks via an online group enabled by WeChat, a popular social instant messaging app in Chinese-speaking communities. The data were collected in three sessions over a single semester using an idiodynamic approach for participants’ ratings of enjoyment intensity and stimulated recall interviews for participants’ descriptions of emotion regulation types. The data were analysed using deductive qualitative thematic analysis and quantitative descriptive analysis. The findings indicate that group-level enjoyment fluctuated within each session of online collaborative learning and tended to last longer in later sessions. Furthermore, the participants reported enacting three different types of regulation – namely self-regulation, co-regulation, and socially shared regulation – to achieve and sustain the dynamic evolution of group-level enjoyment in online collaborative learning. Of these, socially shared regulation was the most prevalent overall, and this increased in prevalence with time. The findings also reveal specific emotion regulation processes within these three types, including planning, evaluating, and the use of emojis. The implications of the findings are discussed, and future research directions are provided.
Politicization of fake news debates and citizen attitudes towards fake news and its regulation
Authors: Hyun Ki Deuk , Seo Mihye , Lee Gunho
Published: January 2024

As fake news becomes a pressing social concern, governments from many countries have considered legislation against fake news. This study examined how citizens formed opinions about fake news and an anti-fake news bill in South Korea where political elites provide polarized discourse regarding fake news and associated regulatory politics. Progressive leaders more intensely criticized fake news and proposed an anti-fake news bill whereas conservative leaders vehemently opposed the bill. The analysis of survey data showed that elite polarization may affect citizens’ perceptions of fake news and attitudes toward anti-fake news legislation. Strong partisans tended to believe that fake news is more hostile toward their in-group and had stronger third-person perceptions, and such perceptions were positively related to the support of anti-fake news legislation. Moreover, progressive voters tended to have stronger hostile and third-person perceptions than conservative counterparts, reflecting endorsement of their in-group leaders’ positions. News reception about the bill further increased the gap in the level of support for the bill between progressive and conservative citizens.
Proximal Row Carpectomy With Soft Tissue Interposition: A Systematic Review of Clinical Outcomes
Authors: Aravinthan Neveadan , Siddiqui Salwa , Khan Moin , Moro Jaydeep , Pino Paula A. , Prada Carlos
Published: January 2024

Proximal row carpectomy (PRC) with soft tissue interposition arthroplasty (STIA) presents an alternative approach to addressing wrist arthritis patterns involving the capitate and/or lunate fossa, in lieu of wrist arthrodesis. This systematic review aimed to evaluate clinical outcomes and techniques associated with PRC-STIA in patients with advanced wrist arthritis. We conducted a systematic review using databases including PubMed, Embase, MEDLINE, and the Cochrane Central Register of Controlled Trials. Inclusion criteria involved articles reporting outcomes of patients who underwent PRC-STIA with at least 1 relevant outcome. The analysis encompassed 8 studies involving 106 patients (108 wrists) meeting the inclusion criteria. A majority of patients were men (69%, n = 88), with a mean age of 54.4 ± 12.7 years and an average follow-up of 4.8 ± 6.3 years. Dorsal capsule was the most commonly interposed tissue (63%, 5 out of 8 studies). Patients receiving STIA achieved comparable patient-reported outcome measures scores to those undergoing PRC alone. Postoperative pain, measured by the Visual Analog Scale, averaged 3.7 ± 0.6. The Disabilities of the Arm, Shoulder, and Hand score averaged 27.8 ± 8, while the Patient-Rated Wrist Evaluation score averaged 41.5 ± 25.9. Five complications were reported in three studies. The addition of STIA into PRC for patients with capitate and/or lunate fossa cartilage degeneration yielded outcomes akin to traditional PRC, improving wrist function, pain, and grip strength in a safe and straightforward manner. Future research should prioritize high-quality comparative studies, extended follow-up periods, and standardized core outcome measures for a more comprehensive understanding of its role in wrist arthritis treatment.
Protectin D1 ameliorates non-compressive lumbar disc herniation through SIRT1-mediated CGRP signaling
Authors: Zhu Yu-Chang , Zhang Yi , Gao Xiao , Li Ling-Xing , Tang Yu-Ru , Wang Yi-Hao
Published: January 2024

Background. Neuro-inflammatory response promotes the initiation and sustenance of lumbar disc herniation (LDH). Protectin D1 (PD1), as a new type of specialized pro-resolving mediator (SPM), can improve the prognosis of various inflammatory diseases. Recent studies have shown that over representation of calcitonin gene-related peptides (CGRP) may activate nociceptive signaling following nerve injury. Silent information regulator 1 (SIRT1) is ubiquitously expressed in the dorsal horn of the spinal cord and plays a role in the pathogenesis of LDH. In this study, we investigated the analgesic effects of PD1 and elucidated the impact of neurogenic inflammation in the pathogenesis of neuropathic pain induced by non-compressive lumbar disc herniation (NCLDH) in a rat model. Methods. NCLDH models were established by applying protruding autologous nucleus pulposus to the L5 Dorsal root ganglion (DRG). PD1, SIRT1 antagonist or agonist, CGRP or antagonist were administered as daily intrathecal injections for three consecutive days postoperatively. Behavioral tests were conducted to assess mechanical and thermal hyperalgesia. The ipsilateral lumbar (L4–6) segment of the spinal dorsal horn was isolated for further analysis. Alterations in the release of SIRT1 and CGRP were explored using western blot and immunofluorescence. Results. Application of protruded nucleus (NP) materials to the DRG induced mechanical and thermal allodynia symptoms, and deregulated the expression of pro-inflammatory and anti-inflammatory cytokines in rats. Intrathecal delivery of PD1 significantly reversed the NCLDH-induced imbalance in neuro-inflammatory response and alleviated the symptoms of mechanical and thermal hyperalgesia. In addition, NP application to the DGRs resulted the spinal upregulation of CGRP and SIRT1 expression, which was almost restored by intrathecal injection of PD1 in a dose-dependent manner. SIRT1 antagonist or agonist and CGRP or antagonist treatment further confirmed the result. Conclusion. Our findings indicate PD1 has a potent analgesic effect, and can modulate neuro-inflammation by regulating SIRT1-mediated CGRP signaling in NCLDH.
Risk Prediction of Diabetic Foot Amputation Using Machine Learning and Explainable Artificial Intelligence
Authors: Oei Chien Wei , Chan Yam Meng , Zhang Xiaojin , Leo Kee Hao , Yong Enming , Chong Rhan Chaen , Hong Qiantai , Zhang Li , Pan Ying , Tan Glenn Wei Leong , Mak Malcolm Han Wen
Published: January 2024

Background:

Diabetic foot ulcers (DFUs) are serious complications of diabetes which can lead to lower extremity amputations (LEAs). Risk prediction models can identify high-risk patients who can benefit from early intervention. Machine learning (ML) methods have shown promising utility in medical applications. Explainable modeling can help its integration and acceptance. This study aims to develop a risk prediction model using ML algorithms with explainability for LEA in DFU patients.

Methods:

This study is a retrospective review of 2559 inpatient DFU episodes in a tertiary institution from 2012 to 2017. Fifty-one features including patient demographics, comorbidities, medication, wound characteristics, and laboratory results were reviewed. Outcome measures were the risk of major LEA, minor LEA and any LEA. Machine learning models were developed for each outcome, with model performance evaluated using receiver operating characteristic (ROC) curves, balanced-accuracy and F1-score. SHapley Additive exPlanations (SHAP) was applied to interpret the model for explainability.

Results:

Model performance for prediction of major, minor, and any LEA event achieved ROC of 0.820, 0.637, and 0.756, respectively, with XGBoost, XGBoost, and Gradient Boosted Trees algorithms demonstrating best results for each model, respectively. Using SHAP, key features that contributed to the predictions were identified for explainability. Total white cell (TWC) count, comorbidity score and red blood cell count contributed highest weightage to major LEA event. Total white cell, eosinophils, and necrotic eschar in the wound contributed most to any LEA event.

Conclusions:

Machine learning algorithms performed well in predicting the risk of LEA in a patient with DFU. Explainability can help provide clinical insights and identify at-risk patients for early intervention.
When Stories Turn Institutional: How TikTok Users Legitimate the Algorithmic Sensemaking
Authors: Obreja Dragoș M.
Published: January 2024

Educational, political, or moral/religious content is increasingly present on TikTok, so contemporary social dynamics legitimize the process of digital mediation regarding these institutional values. Based on 286 open-ended survey answers and subsequent interviews with 45 Romanian TikTok users, this article applies social constructivism to explore the intersubjective side of algorithmic experiences. The significance of such a framework lies in its ability to elucidate the manner in which users actively construct their social environments, which may initially appear as isolated individual experiences but ultimately unveil shared algorithmic interpretations. Thus, the participants highlight three recurrent institutional themes in relation to TikTok’s algorithm: (1) algorithm as political profiling, (2) algorithm as moral plethora, and (3) algorithm as educational benchmark. Findings show that users’ stories related to algorithms are widely conceived within institutional frameworks. These narratives play a role in shaping what Berger and Luckmann call “intersubjective sedimentation” within the intricate interconnection between institutional and algorithmic realities. The ways in which TikTok users legitimize the presence of these institutional actors on their For You page should be seen as a form of agency negotiation between users and machines. The legitimating role of stories about algorithms also highlights the institutional necessity of intergenerational socialization, which is why the contents made by such institutional actors are more and more actively mediated through TikTok.
Message (in)congruence: Tweeting while competing for donations
Authors: Daniel Jamie Levine , Curley Cali , Walk Marlene
Published: January 2024

Nonprofit organizations rely on social media to build relationships with their stakeholders and solicit the resources they need to provide their programs and services. This online activity takes place in an increasingly competitive environment. We draw on the situational theory of publics, stakeholder theory, giving motivation, and gamification to examine this question: When organizations engage in competitive philanthropy, what framing is more effective at generating donations on an online platform? We confirm the relationship between tweeting and donation solicitation and shed light on some specific types of messaging associated with increased donations.
Learning From Experience: Perceptions of Socio-Emotional Development Among German Students During COVID-19
Authors: Crăciun Irina Catrinel
Published: January 2024

The COVID-19 pandemic had an impact on emerging adults’ life and development. The present study explores the socio-emotional development during the corona pandemic from the perspective of German students themselves. Episodic semi-structured interviews were conducted with 30 students and analysed with thematic coding. The main developmental challenges included missed opportunities and experiencing uncertainty, while family support and an optimistic attitude facilitated socioemotional growth. Social developmental gains included increased social support from family and friends and the formation of a generational social identity. Regarding emotional development, self-reflection and self-confidence were enhanced. Findings can be integrated in the Positive Youth Development Theory, as the socio-emotional gains match the 5 C’s (competence, confidence, connection, caring, character). Results have relevant practical implications concerning how to foster positive youth development during uncertain times.
Transforming Political Divides: How Student Identities and Campus Contexts Shape Interpartisan Friendships
Authors: Rockenbach Alyssa N. , Hudson Tara D.
Published: January 2024

Recent evidence suggests that only about 1 in 5 U.S. adults has a friend on the political “other side” (Dunn, 2020). Although these interpartisan friendships are uncommon, they play a critical role in catalyzing empathy, reducing prejudice, furthering justice, and even restoring democracy, as suggested by the theory of civic friendship (Goering, 2003; Kahane, 1999; Rawlins, 2009). In the present study, we drew on national data from the Interfaith Diversity Experiences and Attitudes Longitudinal Survey (IDEALS) to examine the personal and contextual factors that predict interpartisan friendship formation among 5,762 college students attending 118 higher education institutions in the United States. The findings revealed the constellation of individual, social, and institutional contributors to students’ capacities to reach across political differences in their friendships. We offer guidance for how college educators can support the development of these relationships that may open a path toward empathy and healing in our polarized society.
Linking Geographically Mediated Racial Threat and Racial Resentment
Authors: Morris Kevin T.
Published: January 2024

In recent years, the utility of the racial resentment scale for measuring specifically racialized attitudes of white Americans has come into question. This visualization shows that despite these critiques, racial resentment is highest in precisely the parts of the country where geographically mediated threat is most salient: the whitest parts of the least white states. This link between threat and resentment provides a helpful way to think about these two distinct but related theoretical concepts together.
Sustainable development goals and new approaches to HRM: Why HRM specialists will not reach the sustainable development goals and why it matters
Authors: Brewster Chris , Brookes Michael
Published: January 2024

Many of the United Nations’ Sustainable Development Goals apply directly to Human resource management (HRM) within organisations, and most of them have indirect relevance. It is clear, however, that by 2030 the world will have failed to meet the Goals. Although the connection between the SDGs and HRM is not so apparent, it has been argued that the two are, or perhaps should be, related: but maybe there is failure there too. This conceptual paper uses extant research to argue that the reasons for this are inherent in our understanding of HRM, in the nature of the Goals themselves, and in the relationship between HRM and the SDGs. We argue that HRM fails to advance the likelihood of the Goals being met because of construct clarity problems. Nonetheless, we argue that the SDGs have value for HRM specialists in signalling the importance of the multiple stakeholders involved and in focusing attention on crucial aspects of the role of HRM within organisations.
Advantages of an Image-Guided Subcranial Technique for Le Fort III Osteotomies for Midface Advancement: A Case Series
Authors: Holste Katherine G. , Makar Katelyn G. , Ulma Raquel M. , Garton Hugh J.L. , Buchman Steven R. , Vercler Christian J. , Muraszko Karin M.
Published: January 2024

Objective:

Le Fort III osteotomies are used to advance the midface in children with midface hypoplasia and syndromic craniosynostosis. Complications can occur during the subcranial osteotomies, leading todural violations as well as vascular and neurologic injuries. In patients with cerebrospinal fluid (CSF) shunts, CSF leak places them at risk of shunt infection. To avoid these injuries, surgeons may utilize an intracranial approach to gain optimal visualization of the cranial base for the horizontal cut through the ethmoid bone, at the naso-frontal junction, below the cribiform plate. A formal craniotomy to simply assure a safe osteotomy, however, may well be an unnecessary morbidity. The aim of this study was to describe the indications for and technical advantages of image guidance for subcranial Le Fort III osteotomies without a craniotomy.

Methods:

We describe the use of the StealthStation 3-D intraoperative image guidance system for subcranial Le Fort III osteotomies and associated clinical outcomes. An electronic medical record search at a single academic institution identified 10 patients with Current Procedural Terminology codes 21154-21155 (reconstruction midface, Le Fort III).

Results:

Six patients underwent image-guided subcranial Le Fort III osteotomies between 2008 and 2023. There were no cases of vascular injury, cranial nerve injury, or cerebrospinal fluid leak. No patients with a CSF shunt experienced shunt infection during follow up.

Conclusions:

Image-guidance provides technical advantages as an alternative approach to subcranial Le Fort III osteotomies while allowing the surgeon to avoid the morbidity of a craniotomy.
A real-world analysis of clinical outcomes in AML with myelodysplasia-related changes: a comparison of ICC and WHO-HAEM5 criteria
Authors: Zhou Qianghua , Zhao Davidson , Zarif Mojgan , Davidson Marta B. , Minden Mark D. , Tierens Anne , Yeung Yu Wing Tony , Wei Cuihong , Chang Hong
Published: January 2024

The proposed fifth edition of the World Health Organization classification of hematolymphoid tumors (WHO-HAEM5) and International Consensus Classification (ICC) provide different definitions of acute myeloid leukemia with myelodysplasia-related genetics (AML-MR). We conducted a retrospective study which included a cohort of 432 patients, with 354 patients fulfilling WHO-HAEM5 criteria for WHO-AML-MR or 276 patients fulfilling ICC criteria for ICC-AML-MR by gene mutation or cytogenetics (ICC-AML-MR-M/CG). The clinicopathological features were largely similar, irrespective of the classification used, except for higher rates of complex karyotype, monosomy 17, TP53 mutations, and fewer RUNX1 mutations in the WHO-AML-MR group. TP53 mutations were associated with distinct clinicopathological features and dismal outcomes (hazard ratio [HR], 2.98; P < .001). ICC-AML-MR-M/CG group had superior outcome compared with the WHO-AML-MR group (HR, 0.80, P = .032), largely in part due to defining TP53 mutated AML as a standalone entity. In the intensively-treated group, WHO-AML-MR had significantly worse outcomes than AML by differentiation (HR, 1.97; P = .024). Based on ICC criteria, ICC-AML-MR-M/CG had more inferior outcomes compared to AML not otherwise specified (HR, 2.11; P = .048 and HR, 2.55; P = .028; respectively). Furthermore, changing the order of genetic abnormalities defining AML-MR (ie, by gene mutations or cytogenetics) did not significantly affect clinical outcomes. ICC-AML-MR-M/CG showed similar outcomes regardless of the order of assignment. We propose to harmonize the 2 classifications by excluding TP53 mutations from WHO-HAEM5 defined AML-MR group and combining AML-MR defined by gene mutations and cytogenetics to form a unified group.
Genome-wide association study, population structure, and genetic diversity of the tea plant in Guizhou Plateau
Authors: Wang Yihan , Niu Suzhen , Deng Xinyue , Bai Dingchen , Chen Zhengwu , Deng Xiuling , Huang Dejun
Published: January 2024

Background

Guizhou Plateau, as one of the original centers of tea plant, has a profound multi-ethnic cultural heritage and abundant tea germplasm resources. However, the impact of indigenous community factors on the genetic diversity, population structure and geographical distribution of tea plant is still unclear.

Results

Using the genotyping-by-sequencing (GBS) approach, we collected 415 tea plant accessions from the study sites, estimated genetic diversity, developed a core collection, and conducted a genome-wide association study (GWAS) based on 99,363 high-quality single-nucleotide polymorphisms (SNPs). A total of 415 tea accessions were clustered into six populations (GP01, GP02, GP03, GP04, GP05 and GP06), and the results showed that GP04 and GP05 had the highest and lowest genetic diversity (Pi = 0.214 and Pi = 0.145, respectively). Moreover, 136 tea accessions (33%) were selected to construct the core set that can represent the genetic diversity of the whole collection. By analyzing seven significant SNP markers associated with the traits such as the germination period of one bud and two leaves (OTL) and the germination period of one bud and three leaves (OtL), four candidate genes possibly related to OTL and OtL were identified.

Conclusions

This study revealed the impact of indigenous communities on the population structure of 415 tea accessions, indicating the importance of cultural practices for protection and utilization of tea plant genetic resources. Four potential candidate genes associated with the OTL and OtL of tea plant were also identified, which will facilitate genetic research, germplasm conservation, and breeding.
German translation and validation of the complementary and alternative medicine health belief questionnaire (CHBQ)
Authors: Hinse Maximilian , Jerzynski Lea , Binting Sylvia , Kummer Sonja , Brinkhaus Benno , Rotter Gabriele
Published: January 2024

Background

The Complementary and Alternative Health Belief Questionnaire (CHBQ) measures medical students’ attitudes towards Complementary Medicine (CM). The aim of the study was to examine the validity and reliability of the German translation of the CHBQ.

Methods

Data for the psychometric evaluation of the German translation were drawn from a study that investigated attitudes towards CM in (a sample of) medical students at Charité - Universitätsmedizin Berlin. Construct validity was determined via an exploratory factor analysis (EFA). Internal consistency was examined using Cronbach’s alpha and split-half reliability.

Results

The CHBQ was returned by 278 students, and was fully completed by 260 students (mean age 23.7 years; ± 4.3 SD), 69.2% were female). EFA revealed a single factor solution for all 10 items of the scale. All items, except one, had good item discrimination (range: 0.5–0.8), acceptable mean inter-item-correlation (0.39) and similar median correlation (0.38). Reliability was very good (α = 0.86) and further confirmed by split-half reliability (0.91).

Conclusions

The German version of the CHBQ is a valid and reliable instrument for measuring students’ attitudes towards CM.
Informed consent practice and associated factors among healthcare professionals in public hospitals of Southern Ethiopia, 2023: a mixed-method study
Authors: Bolado Getachew Nigussie , Ataro Bizuayehu Atnafu , Feleke Mulualem Gete , Gadabo Christian Kebede , Kebamo Tamirat Ersino , Minuta Worku Mimani
Published: January 2024

Background

Patients may sign a consent form before the specific treatment is offered for a variety of reasons, including during an outpatient appointment. Healthcare professionals must obtain consent from patients or other legal persons before providing any treatment or performing any procedures. But, little attention has been given to the informed consent process in Ethiopia.

Objective

To assess informed consent practice and associated factors among healthcare professionals in Wolaita Zone, Southern Ethiopia public hospitals from January, 2023.

Methods

An institutional-based cross-sectional mixed-method study was conducted among 399 healthcare professionals. Simple random sampling and purposive sampling techniques were used to select healthcare professionals for quantitative and qualitative studies respectively. Data for both studies were collected using self-administered questionnaire and key informant interview respectively. EpiDataV4.6 and the Statistical Package for the Social Science was used for entry and analysis of quantitative data. OpenCode software was used for thematic analysis for qualitative data.

Results

339 respondents were included in the study, with a response rate of 94.3%. The good practice of informed consent among the healthcare professionals is 53.1%. There was a significant association between the good practice of informed consent and being male [AOR: 0.003 (95% CI: 0.000–0.017)], working in a comprehensive specialized hospital [AOR: 4.775 (95% CI: 1.45–15.74)] and in-service training [AOR: 0.038 (95% CI: 0.013–0.114)].

Conclusion and recommendations

More than half of healthcare professionals had good practices for informed consent. However, it is critical to plan and intervene various strategies with the goal of improving knowledge and attitude toward informed consent.
Clinical nursing mentors’ motivation, attitude, and practice for mentoring and factors associated with them
Authors: Wang Yan , Hu Suzhen , Yao Jiali , Pan Yangmiao , Wang Junling , Wang Hua
Published: January 2024

Objective

To investigate the motivation, attitude, and practice toward mentoring and related factors among clinical nursing mentors.

Methods

This cross-sectional study included clinical nursing mentors from 30 hospitals in Zhejiang Province between August and September 2023. Demographic information, motivation, attitude, and practice were collected through a self-administered questionnaire.

Results

A total of 495 valid questionnaires were collected, and most of the participants were 30–39 years old (68.7%). Average motivation, attitude, and practice scores were 29 [26, 32] (possible range: 8–40), 87 (82, 94) (possible range: 22–110), and 41 (38, 45) (possible range: 11–55), respectively. Correlation analyses showed that the motivation scores were positively correlated with attitude scores (r = 0.498, P < 0.001) and practice scores (r = 0.408, P = 0.001), while attitude scores were positively correlated with practice scores (r = 0.554, P < 0.001). Multivariate logistic regression showed that intermediate and senior nursing mentors (OR = 0.638, 95% CI: [0.426–0.956], P = 0.030) and different hospitals (OR = 1.627, 95% CI: [1.054–2.511], P = 0.028) were independently associated with motivation. The hospital’s frequency of psychological care was a significant factor associated with nursing mentoring motivation, attitude, and practice. Participation in training (OR = 2.908, 95% CI: [1.430, 5.913], P = 0.003) and lower frequency of job evaluation in hospital (“Often”: OR = 0.416, 95% CI: [0.244–0.709], P = 0.001 and “Sometimes”: OR = 0.346, 95% CI: [0.184–0.650], P = 0.001) were independently associated with practice.

Conclusion

Clinical nursing mentors had adequate motivation, positive attitude, and proactive practice towards mentoring and associated factors. Clinical nursing mentorship should be enhanced by prioritizing mentor training, fostering a supportive environment with consistent psychological care, and promoting structured mentorship activities.
Development and pilot-testing of an evidence-based quality indicator set for home mechanical ventilation care: the OVER-BEAS project
Authors: Schutzmeier Martha , Brandstetter Lilly Sophia , Stangl Stephanie , Ahnert Jutta , Grau Anna , Gerken Laura , Klingshirn Hanna , Reuschenbach Bernd , Skazel Tobias , Kippnich Maximilian , Wurmb Thomas , Heuschmann Peter , Haas Kirsten
Published: January 2024

Background

The number of patients depending on home mechanical ventilation (HMV) has increased substantially in Germany in recent years. These patients receive long-term care in different nursing facilities (nursing home, shared living community, private home). However, there are limited data available on the quality of care of HMV patients. The aim of the OVER-BEAS project was to identify quality indicators (QIs) of HMV care using an evidence-based approach.

Methods

A multidisciplinary board consisting of professionals and experts of HMV provision compiled a set of QIs between March and September 2019. In a structured, transparent process a set of QIs covering structures, processes and outcome of HMV patient’s care were proposed and evaluated based on the best available evidence. QIs were defined as relevant, reliable and valid measurements of the quality of HMV care and furthermore to be comprehensive and applicable in practice.

Results

The experts proposed 40 QIs and consented a final set of 26 QIs. Based on the final set, questionnaires to document the QIs were developed: (1) to assess the quality and describe the structure of the nursing facility; and (2) to gather information on patient-related processes and outcomes. The feasibility of the questionnaires was tested in 5 nursing facilities treating HMV patients. The remarks from the nursing specialists were categorised in three groups: (1) term missing accuracy, (2) problem of understanding, and (3) not documented or documented elsewhere. Mean documentation time by the nursing specialists for one patient was 15 min. Based on this feedback, the questionnaires were finalised.

Conclusions

We proposed a set of QIs relating to long-term HMV care and developed two questionnaires to collect this information. In a pilot study, we found the set of questionnaires to be feasible in assessing the quality of HMV care according to current evidence. The development of standardised evidence-based QIs to evaluate HMV care is a step towards implementing a standardised quality assurance program to document the quality of care of HMV patients.
Pediococcus acidilactici reduces tau pathology and ameliorates behavioral deficits in models of neurodegenerative disorders
Authors: Zhang Yong , Qian Weiyi , Zhang Yitong , Ma Yan , Qian Jiamin , Li Jinping , Wei Xun , Long Yan , Wan Xiangyuan
Published: January 2024

Background

Alzheimer’s disease (AD), affecting many elders worldwide, is characterized by A-beta and tau-related cognitive decline. Accumulating evidence suggests that brain iron accumulation is an important characteristic of AD. However, the function and mechanism of the iron-mediated gut-brain axis on AD is still unclear.

Methods

A Caenorhabditis elegans model with tau-overexpression and a high-Fe diet mouse model of cognitive impairment was used for probiotic function evaluation. With the use of qPCR, and immunoblotting, the probiotic regulated differential expression of AD markers and iron related transporting genes was determined. Colorimetric kits, IHC staining, and immunofluorescence have been performed to explore the probiotic mechanism on the development of gut-brain links and brain iron accumulation.

Results

In the present study, a high-Fe diet mouse model was used for evaluation in which cognitive impairment, higher A-beta, tau and phosphorylated (p)-tau expression, and dysfunctional phosphate distribution were observed. Considering the close crosstalk between intestine and brain, probiotics were then employed to delay the process of cognitive impairment in the HFe mouse model. Pediococcus acidilactici (PA), but not Bacillus subtilis (BN) administration in HFe-fed mice reduced brain iron accumulation, enhanced global alkaline phosphatase (AP) activity, accelerated dephosphorylation, lowered phosphate levels and increased brain urate production. In addition, because PA regulated cognitive behavior in HFe fed mice, we used the transgenic Caenorhabditis elegans with over-expressed human p-tau for model, and then PA fed worms became more active and longer lived than E.coli fed worms, as well as p-tau was down-regulated. These results suggest that brain iron accumulation influences AD risk proteins and various metabolites. Furthermore, PA was shown to reverse tau-induced pathogenesis via iron transporters and AP-urate interaction.

Conclusions

PA administration studies demonstrate that PA is an important mediator of tau protein reduction, p-tau expression and neurodegenerative behavior both in Caenorhabditis elegans and iron-overload mice. Finally, our results provide candidates for AP modulation strategies as preventive tools for promoting brain health.
Medication use during pregnancy and the risk of gastroschisis: a systematic review and meta-analysis of observational studies
Authors: Baldacci Silvia , Santoro Michele , Mezzasalma Lorena , Pierini Anna , Coi Alessio
Published: January 2024

Objectives

The aetiology of gastroschisis is considered multifactorial. We conducted a systematic review and meta-analysis to assess whether the use of medications during pregnancy, is associated with the risk of gastroschisis in offspring.

Methods

PubMed, EMBASE, and Scopus were searched from 1st January 1990 to 31st December 2020 to identify observational studies examining the association between medication use during pregnancy and the risk of gastroschisis. The Newcastle–Ottawa Scale was used for the quality assessment of the individual studies. We pooled adjusted measures using a random-effect model to estimate relative risk [RR] and the 95% confidence interval [CI]. I2 statistic for heterogeneity and publication bias was calculated.

Results

Eighteen studies providing data on 751,954 pregnancies were included in the meta-analysis. Pooled RRs showed significant associations between aspirin (RR 1.66, 95% CI 1.16–2.38; I2 = 58.3%), oral contraceptives (RR 1.52, 95% CI 1.21–1.92; I2 = 22.0%), pseudoephedrine and phenylpropanolamine (RR 1.51, 95% CI 1.16–1.97; I2 = 33.2%), ibuprofen (RR 1.42, 95% CI 1.26–1.60; I2 = 0.0%), and gastroschisis. No association was observed between paracetamol and gastroschisis (RR 1.16, 95% CI 0.96–1.41; I2 = 39.4%).

Conclusions

These results suggest that the exposure in the first trimester of pregnancy to over the counter medications (OTC) such as aspirin, ibuprofen, pseudoephedrine and phenylpropanolamine as well as to oral contraceptives, was associated with an increased risk of gastroschisis. However, these associations are significant only in particular subgroups defined by geographic location, adjustment variables and type of control. Therefore, further research is needed to investigate them as potential risk factors for gastroschisis, to assess their safety in pregnancy and to develop treatment strategies to reduce the risk of gastroschisis in offspring.

PROSPERO registration number: CRD42021287529.
Knockout of a PLD gene in Schizochytrium limacinum SR21 enhances docosahexaenoic acid accumulation by modulation of the phospholipid profile
Authors: Zhang Yiting , Cui Xiaowen , Lin Shuizhi , Lu Tao , Li Hao , Lu Yinghua , Cao Mingfeng , Lin Xihuang , Ling Xueping
Published: January 2024

Background

The hydrolysis and transphosphatidylation of phospholipase D (PLD) play important roles in the interconversion of phospholipids (PLs), which has been shown to profoundly impact lipid metabolism in plants. In this study, the effect of the PLD1 gene of Schizochytrium limacinum SR21 (S. limacinum SR21) on lipid metabolism was investigated.

Results

PLD1 knockout had little impact on cell growth and lipid production, but it significantly improved the percentage of polyunsaturated fatty acids in lipids, of which docosahexaenoic acid (DHA) content increased by 13.3% compared to the wild-type strain. Phospholipomics and real-time quantitative PCR analysis revealed the knockout of PLD1 reduced the interexchange and increased de novo synthesis of PLs, which altered the composition of PLs, accompanied by a final decrease in phosphatidylcholine (PC) and an increase in phosphatidylinositol, lysophosphatidylcholine, and phosphatidic acid levels. PLD1 knockout also increased DHA content in triglycerides (TAGs) and decreased it in PLs.

Conclusions

These results indicate that PLD1 mainly performs the transphosphatidylation activity in S. limacinum SR21, and its knockout promotes the migration of DHA from PLs to TAGs, which is conducive to DHA accumulation and storage in TAGs via an acyl CoA-independent pathway. This study provides a novel approach for identifying the mechanism of DHA accumulation and metabolic regulation strategies for DHA production in S. limacinum SR21.
Species identification of adult ixodid ticks by Raman spectroscopy of their feces
Authors: Dou Tianyi , Holman Aidan P. , Hays Samantha R. , Donaldson Taylor G. , Goff Nicolas , Teel Pete D. , Kurouski Dmitry
Published: January 2024

Background

Ticks and tick-borne diseases pose significant challenges to cattle production, thus the species identification of ticks and knowledge on their presence, abundance, and dispersal are necessary for the development of effective control measures. The standard method of inspection for the presence of ticks is the visual and physical examination of restrained animals, but the limitations of human sight and touch can allow larval, nymphal, and unfed adult ticks to remain undetected due to their small size and site of attachment. However, Raman spectroscopy, an analytical tool widely used in agriculture and other sectors, shows promise for the identification of tick species in infested cattle. Raman spectroscopy is a non-invasive and efficient method that employs the interaction between molecules and light for the identification of the molecular constituents of specimens.

Methods

Raman spectroscopy was employed to analyze the structure and composition of tick feces deposited on host skin and hair during blood-feeding. Feces of 12 species from a total of five genera and one subgenus of ixodid ticks were examined. Spectral data were subjected to partial least squares discriminant analysis, a machine-learning model. We also used Raman spectroscopy and the same analytical procedures to compare and evaluate feces of the horn fly Haematobia irritans after it fed on cattle.

Results

Five genera and one sub-genus at overall true prediction rates ranging from 92.3 to 100% were identified from the Raman spectroscopy data of the tick feces. At the species level, Dermacentor albipictus, Dermacentor andersoni and Dermacentor variabilis at overall true prediction rates of 100, 99.3 and 100%, respectively, were identified. There were distinct differences between horn fly and tick feces with respect to blood and guanine vibrational frequencies. The overall true prediction rate for the separation of tick and horn fly feces was 98%.

Conclusions

Our findings highlight the utility of Raman spectroscopy for the reliable identification of tick species from their feces, and its potential application for the identification of ticks from infested cattle in the field.

Graphical Abstract
Anti-Toxoplasma gondii antibodies as a risk factor for the prevalence and severity of systemic lupus erythematosus
Authors: Li Zhongzhen , Lei Zhiwei , Yang Wanying , Jing Chunxia , Sun Xiaolin , Yang Guang , Zhao Xiaozhen , Zhang Mingjiao , Xu Miaomiao , Tang Yuanjia , Wang Qingwen , Zhao Jing , Zhou Zixing , Wen Zihao , Chen Xiaojing , Peng Qinglin , Wang Guochun , Zhang Pingjing , Sun Erwei , Shen Nan , Xu Weiguo , Li Zhanguo , Yang Hengwen , Yin Zhinan
Published: January 2024

Background

Systemic lupus erythematosus (SLE) is a complex systemic autoimmune disease characterized by the presence of numerous autoantibodies. The interaction of infectious agents (viruses, bacteria and parasites) and a genetically susceptible host may be a key mechanism for SLE. Toxoplasma gondii is a widespread intracellular parasite that has been implicated in the pathogenesis of autoimmune diseases. However, the relationship between T. gondii infection and the increased risk of SLE in Chinese populations remains unclear.

Methods

The seroprevalence of T. gondii infection was assessed in 1771 serum samples collected from Chinese individuals (908 healthy controls and 863 SLE patients) from different regions of China using an enzyme-linked immunosorbent assay. Serum autoantibodies and clinical information were obtained and analysed.

Results

Our observations revealed a higher prevalence of anti-T. gondii antibodies (ATxA) immunoglobulin G (IgG) in serum samples from SLE patients (144/863, 16.7%) than in those from the healthy controls (53/917, 5.8%; P < 0.0001), indicating a 2.48-fold increased risk of SLE in the ATxA-IgG+ population, after adjustment for age and sex (95% confidence interval [CI] 1.70–3.62, P < 0.0001). ATxA-IgG+ SLE patients also showed a 1.75-fold higher risk of developing moderate and severe lupus symptoms (95% CI 1.14–2.70, P = 0.011) compared to ATxA-IgG− patients. Relative to ATxA-IgG− patients, ATxA-IgG+ patients were more likely to develop specific clinical symptoms, including discoid rash, oral ulcer, myalgia and alopecia. Seven antibodies, namely anti-ribosomal RNA protein (rRNP), anti-double stranded DNA (dsDNA), anti-cell membrane DNA (cmDNA), anti-scleroderma-70 (Scl-70), anti-cardiolipin (CL), anti-beta2-glycoprotein-I (B2GPI) and rheumatoid factor (RF), occurred more frequently in ATxA-IgG+ patients. When combined with anti-dsDNA and RF/anti-rRNP/anti-cmDNA/ESR, ATxA-IgG significantly increased the risk for severe lupus.

Conclusions

Our results suggest that ATxA-IgG may be a significant risk factor for SLE prevalence and severity in Chinese populations.

Graphical Abstract
Advancements in diagnostic and interventional radiology for stroke treatment: the path from trial to bedside through the pre-MR CLEAN, MR CLEAN, and MR CLEAN II eras
Authors: Samuels Noor , van de Graaf Rob A. , Roos Yvo B. W. M. , Dippel Diederik , van der Lugt Aad ,   the MR CLEAN and CONTRAST investigators
Published: January 2024

The stroke field is inevitably connected with imaging in which radiologists fulfill a central role. Our landmark MR CLEAN trial led to the implementation of baseline computed tomography angiography or magnetic resonance angiography in the acute stroke workup and subsequent endovascular treatment (EVT) for ischemic stroke patients with a large vessel occlusion in the anterior circulation, resulting in numerous patients worldwide currently being treated often successfully. A reversal of the pathophysiologic process behind an acute cerebrovascular event was made possible. Subsequently, in the MR CLEAN II trials, the clinical impact of both diagnostic and interventional radiologists remained a cornerstone of our research, which means value-based radiology. Within these MR CLEAN II trials, we proved that aspirin and heparin during EVT should be avoided due to increased symptomatic intracranial hemorrhage risk (MR CLEAN-MED). We concluded there is additional benefit of EVT in the 6-to-24-h window after stroke in the presence of good collaterals on baseline CTA (MR CLEAN-LATE). The impactful success of our stroke trials that changed many guidelines was mainly attributable to (1) the societal burden of the disease, with two thirds of patients dying or being independent at 3 months; (2) the fact that stroke is a common disease, (3) the relatively simple and pragmatic approach of the trials resembling real-world setting; (4) the acceleration of implementation in clinical practice facilitated by a structured approach to guideline development and conditional funding; and foremost (5) the excellent collaboration on a professional level between-disciplines, i.e., diagnostic radiologists, interventionalists, and neurologists.

Critical relevance statement The MR CLEAN and MR CLEAN II trials have had tremendous impact on clinical practice, directly by more patients being treated with an effective intervention and indirectly through adoption of evidence-based guidelines. It is in this setting of stroke treatment that diagnostic and interventional radiologists have played a crucial role and created clinical impact.
Study on the stability and its simulation algorithm of a nonlinear impulsive ABC-fractional coupled system with a Laplacian operator via F-contractive mapping
Authors: Zhao Kaihong
Published: January 2024

In this paper, we study the solvability and generalized Ulam–Hyers (UH) stability of a nonlinear Atangana–Baleanu–Caputo (ABC) fractional coupled system with a Laplacian operator and impulses. First, this system becomes a nonimpulsive system by applying an appropriate transformation. Secondly, the existence and uniqueness of the solution are obtained by an F-contractive operator and a fixed-point theorem on metric space. Simultaneously, the generalized UH-stability is established based on nonlinear analysis methods. Thirdly, a novel numerical simulation algorithm is provided. Finally, an example is used to illustrate the correctness and availability of the main results. Our study is a beneficial exploration of the dynamic properties of viscoelastic turbulence problems.
An enhanced state-aware model learning approach for security analysis in lightweight protocol implementations
Authors: Guo Jiaxing , Zhao Dongliang , Gu Chunxiang , Chen Xi , Zhang Xieli , Ju Mengcheng
Published: January 2024

Owing to the emergence and rapid advances of new-generation information and digitalization technologies, the concept of model-driven digital twin has received widespread attentions and is developing vigorously. Driven by data and simulators, the digital twin can create the virtual twins of physical objects to perform monitoring, simulation, prediction, optimization, and so on. Hence, the application of digital twin can increase efficiency and security of systems by providing reliable model and decision supports. In this paper, we propose a state-aware model learning method to simulate and analyze the lightweight protocol implementations in edge/cloud environments. We introduce the data flow of program execution and network interaction inputs/outputs (I/O) into the extended finite state machine (EFSM) to expand the modeling scope and insight. We aim to calibrate the states and construct an accurate state-machine model using a digital twin based layered approach to reasonably reflect the correlation of a device’s external behavior and internal data. This, in turn, improves our ability to verify the logic and evaluate the security for protocol implementations. This method firstly involves instrumenting the target device to monitor variable activity during its execution. We then employ learning algorithms to produce multiple rounds of message queries. Both the I/O data corresponding to these query sequences and the state calibration information derived from filtered memory variables are obtained through the mapper and execution monitor, respectively. These two aspects of information are combined to dynamically and incrementally construct the protocol’s state machine. We apply this method to develop SALearn and evaluate the effectiveness of SALearn on two lightweight protocol implementations. Our experimental results indicate that SALearn outperforms existing protocol model learning tools, achieving higher learning efficiency and uncovering more interesting states and security issues. In total, we identified two violation scenarios of rekey logic. These situations also reflect the differences in details between different implementations.