bluuebunny/crossref_metadata_2025 huggingface dataset:
Community Structure and Functional Role of Limber Pine (Pinus flexilis) in Treeline Communities in Rocky Mountain National Park
Authors: Sindewald Laurel A. , Tomback Diana F. , Neumeyer Eric R.
Published: August 2020

Research Highlights: Limber pine (Pinus flexilis) is abundant in some alpine treeline ecotone (ATE) communities east of the Continental Divide in Rocky Mountain National Park (RMNP) and the Colorado Front Range. Limber pine may be able to colonize the ATE under changing climate aided by directed seed dispersal by Clark’s nutcrackers (Nucifraga columbiana). Cronartium ribicola, white pine blister rust, is a growing threat to limber pine and may affect its functional role within the ATE. Background and Objectives: The ATE is sensitive, worldwide, to increasing temperature. However, the predicted advance of treeline under a changing climate may be modified by tree species composition and interactions. We aimed to (1) examine the conifer species composition and relative abundances in treeline communities with limber pine; (2) assess which functional roles limber pine assumes in these communities—tree island initiator, tree island component, and/or solitary tree; and (3) determine whether limber pine’s occurrence as a tree island initiator can be predicted by its relative abundance as a solitary tree. Materials and Methods: We selected four study sites in RMNP above subalpine forest limber pine stands. We sampled the nearest tree island to each of forty random points in each study site as well as solitary tree plots. Results: Across study sites, limber pine comprised, on average, 76% of solitary trees and was significantly more abundant as a solitary tree than Engelmann spruce (Picea engelmannii) or subalpine fir (Abies lasiocarpa). Limber pine was a frequent component of multi-tree islands in three study sites, the major component in one study site, and dominated single-tree islands at two study sites. At three of four study sites, no species had significantly greater odds of being a tree island initiator. Limber pine was found less often as a tree island initiator than predicted from its relative abundance as a solitary tree, given the likely role of solitary trees in tree island formation.
Long-Term Seeding Outcomes in Slash Piles and Skid Trails after Conifer Removal
Authors: Kerns Becky K. , Day Michelle A. , Ikeda Dana
Published: August 2020

Conifer removal in interior woodland ecosystems of the western US is a common management treatment used to decrease fire hazard and shift woodlands to more historical states. Woody material is frequently removed by skidding material off site and via slash pile burning. Assessing the long-term outcomes of seeding treatments after such ground disturbing activities is critical for informing future management and treatment strategies. Using two designed experiments from a central Oregon juniper woodland, we resampled slash piles and skid trails 8 years after seeding. Our objectives were to assess the long-term vegetation response to conifer removal, ground disturbance, and seeding source (cultivar and local) in slash piles and skid trails. We found that seeded species persisted in the long term, but abundance patterns depended on the species, seed source, and the type of disturbance. In general, there were more robust patterns of persistence after pile burning compared to skid trails. Seeding also suppressed exotic grass cover in the long term, particularly for the local seed source. However, the invasion levels we report are still problematic and may have impacts on biodiversity, forage and fire behavior. Our short-term results were not predictive of longer-term outcomes, but short- and long-term patterns were somewhat predictable based on species life history traits and ecological succession. The use of a mix of species with different life history traits may contribute to seeding success in terms of exotic grass suppression. Lastly, our results suggest that locally adapted seed sources may perform as well or better compared to cultivars. However, more aggressive weed treatments before and after conifer removal activities and wider seeding application may be needed to effectively treat exotic grass populations.
Growth, Carbon Storage, and Optimal Rotation in Poplar Plantations: A Case Study on Clone and Planting Spacing Effects
Authors: Zhang Yanhua , Tian Ye , Ding Sihui , Lv Yi , Samjhana Wagle , Fang Shengzuo
Published: August 2020

Poplar, as the most widely cultivated fast-growing tree species in the middle latitude plain, provides important wood resources and plays an important role in mitigating climate change. In order to understand the response of growth, biomass production, carbon storage to poplar clones, planting spacings, and their interaction, a field trial was established in 2007. In 2018, we destructively harvested 24 sample trees for biomass measurements and stem analyses. Biomass production and carbon storage for the single tree of three clones enhanced as planting spacing increasing at the age of 13, but both the biomass production and carbon storage of clones NL-895 and NL-95 were higher than the clone NL-797 at the spacings of 6 × 6 m and 5 × 5 m. The average carbon concentration of the tested clones was in the order of stem > branches > leaves, and showed significant variation between different components (p < 0.05). Large spacing stimulated more biomass to be partitioned to the canopy. Based on the prediction values of tree volume growth by established Chapman–Richards models, the quantitative maturity ages of stand volume varied among the investigating plantations, ranging from 14 to 17 years old. Our results suggest that the selecting clones NL-895 and NL-95 with 6 × 6 m spacing would be recommended at similar sites for future poplar silviculture of larger diameter timber production, as well as for carbon sequestration.
Comparison of the Glycolytic and Alcoholic Fermentation Pathways of Hanseniaspora vineae with Saccharomyces cerevisiae Wine Yeasts
Authors: Valera María José , Boido Eduardo , Dellacassa Eduardo , Carrau Francisco
Published: August 2020

Hanseniaspora species can be isolated from grapes and grape musts, but after the initiation of spontaneous fermentation, they are displaced by Saccharomyces cerevisiae. Hanseniaspora vineae is particularly valuable since this species improves the flavour of wines and has an increased capacity to ferment relative to other apiculate yeasts. Genomic, transcriptomic, and metabolomic studies in H. vineae have enhanced our understanding of its potential utility within the wine industry. Here, we compared gene sequences of 12 glycolytic and fermentation pathway enzymes from five sequenced Hanseniaspora species and S. cerevisiae with the corresponding enzymes encoded within the two sequenced H. vineae genomes. Increased levels of protein similarity were observed for enzymes of H. vineae and S. cerevisiae, relative to the remaining Hanseniaspora species. Key differences between H. vineae and H. uvarum pyruvate kinase enzymes might explain observed differences in fermentative capacity. Further, the presence of eight putative alcohol dehydrogenases, invertase activity, and sulfite tolerance are distinctive characteristics of H. vineae, compared to other Hanseniaspora species. The definition of two clear technological groups within the Hanseniaspora genus is discussed within the slow and fast evolution concept framework previously discovered in these apiculate yeasts.
Bonding Wood Veneer with Biobased Poly(Lactic Acid) Thermoplastic Polyesters: Potential Applications for Consolidated Wood Veneer and Overlay Products
Authors: Grigsby Warren J. , Puri Arpit , Gaugler Marc , Lüedtke Jan , Krause Andreas
Published: August 2020

This study reports on the use of poly(lactic acid) (PLA) as a renewable thermoplastic adhesive for laminated panels using birch, spruce, and pine veneers. Consolidated panels were prepared from veneer and PLA foils by hot-pressing from 140 to 180 °C to achieve minimum bondline temperatures. Evaluation of panel properties revealed that the PLA-bonded panels met minimum tensile strength and internal bond strength performance criteria. However, the adhesion interface which developed within individual bondlines varied with distinctions between hardwood and softwood species and PLA grades. Birch samples developed greater bondline strength with a higher pressing temperature using semi-crystalline PLA, whereas higher temperatures produced a poorer performance with the use of amorphous PLA. Panels formed with spruce or pine veneers had lower bondline performance and were also similarly distinguished by their pressing temperature and PLA grade. Furthermore, the potential for PLA-bonded laminated panels was demonstrated by cold water soak testing. Samples exhibiting relatively greater bondline adhesion had wet tensile strength values comparable to those tested in dry state. Our study outcomes suggest the potential for PLA bonding of veneers and panel overlays with the added benefits of being renewable and a no added formaldehyde system.
Modern Methods for Assessing the Quality of Bee Honey and Botanical Origin Identification
Authors: Puścion-Jakubik Anna , Borawska Maria Halina , Socha Katarzyna
Published: August 2020

This paper is a summary of the latest literature on methods for assessing quality of natural bee honey. The publication briefly characterizes methods recommended by the International Honey Commission, published in 2009, as well as newer methods published in the last 10 years. Modern methods of assessing honey quality focus mainly on analyzing markers of individual varieties and classifying them into varieties, using, among others, near infrared spectroscopy techniques (NIR), potentiometric tongue, electronic nose, nuclear magnetic resonance (NMR), zymography, polymerase chain reaction (PCR), DNA metabarcoding, and chemometric techniques including partial least squares (PLS), principal component analysis (PCA) and artificial neural networks (ANN). At the same time, effective techniques for analyzing adulteration, sugar, and water content, hydroxymethylfurfural (HMF), polyphenol content, and diastase activity are being sought. Modern techniques enable the results of honey quality testing to be obtained in a shorter time, using the principles of green chemistry, allowing, at the same time, for high precision and accuracy of determinations. These methods are constantly modified, so that the honey that is on sale is a product of high quality. Prospects for devising methods of honey quality assessment include the development of a fast and accurate alternative to the melissopalynological method as well as quick tests to detect adulteration.
In Vivo Anti-Inflammatory Potential of Viscozyme®-Treated Jujube Fruit
Authors: Kim Yoonsu , Oh Jisun , Jang Chan Ho , Lim Ji Sun , Lee Jeong Soon , Kim Jong-Sang
Published: August 2020

The fruit of Ziziphus jujuba, commonly called jujube, has long been consumed for its health benefits. The aim of this study was to examine the protective effect of dietary supplementation of enzymatically hydrolyzed jujube against lung inflammation in mice. The macerated flesh of jujube was extracted with aqueous ethanol before and after Viscozyme treatment. The extract of enzyme-treated jujube, called herein hydrolyzed jujube extract (HJE), contained higher levels of quercetin, total phenolics, and flavonoids, and exhibited more effective radical-scavenging abilities in comparison to non-hydrolyzed jujube extract (NHJE). HJE treatment decreased production of inflammation-associated molecules, including nitric oxide and pro-inflammatory cytokines from activated Raw 264.7 or differentiated THP-1 cells. HJE treatment also reduced expression of nuclear factor-κB and its downstream proteins in A549 human lung epithelial cells. Moreover, oral supplementation of 1.5 g of HJE per kg of body weight (BW) attenuated histological lung damage, decreased plasma cytokines, and inhibited expression of inflammatory proteins and oxidative stress mediators in the lungs of mice exposed to benzo(a)pyrene at 50 mg/kg BW. Expression levels of antioxidant and cytoprotective factors, such as nuclear factor erythroid-derived 2-related factor 2 and heme oxygenase-1, were increased in lung and liver tissues from mice treated with HJE, compared to mice fed NHJE. These findings indicate that dietary HJE can reduce benzo(a)pyrene-induced lung inflammation by inhibiting cytokine release from macrophages and promoting antioxidant defenses in vivo.
What Do We Mean by “Ethnicity” and “Race”? A Consensual Qualitative Research Investigation of Colloquial Understandings
Authors: Suyemoto Karen L. , Curley Micaela , Mukkamala Shruti
Published: August 2020

Lack of clarity and questionable congruence between researcher and participant understandings of ethnicity and race challenge the validity and impact of research utilizing these concepts. We aimed to both elucidate the multiple meanings that research participants in the United States might bring to questions about ethnicity and race and examine their relation to formal conceptualizations of these variables. We used consensual qualitative research-modified analyses to conduct thematic content analysis of 151 responses to open-ended survey questions about meanings of ethnicity and race. Participants included a racially diverse sample of 53 males, 87 females, and 11 unidentified gender with a mean age of 28.71 years. Results indicated that the most frequent colloquial meanings of ethnicity included origin, culture, ancestry, related or similar to race, social similarity, religion, and identity. The most frequent colloquial meanings of race included physical characteristics, ethnicity, origin, social grouping, ancestry, and imposed categorization. Results also illustrated how participants approached defining ethnicity and race. Results support the acknowledged and critiqued colloquial confounding of ethnicity and race and indicate a lack of agreed upon meaning between lay representations/meanings and formal meanings used by social scientists. This incongruence threatens valid operationalizations for research and challenges our ability to use these concepts in interventions to promote social justice and psychological health.
Racialized Affectivities of (Un)Belonging: Mixed (Race) Couples in the Shadow of Brexit
Authors: Zambelli Elena
Published: August 2020

This paper explores the affective economy of (un)belonging, revealed by the UK decision to withdraw from the European Union (EU). Emerging social science research on so-called ‘Brexit’ focuses on the anticipated effects of a stricter UK immigration regime on the lives of EU citizens and families. Against the background of the country’s postcolonial melancholia, and drawing from my ethnographic fieldwork in England (2018–2019), this paper discusses how British and mixed-migration status, mixed (race) couples narrate the impact of the poll’s outcome on their affective orientations towards the UK and the EU. It shows how race inflects partners’ different perception of Brexit as a historical rupture or as an event in a continuum; as a loss of entitlement to mobility in space, or of the legitimacy of permanence in place; as a lingering danger, or a magnifier of existing patterns of violence. By putting Black and mixed-race partners’ narratives center stage, this paper traces three scenes of expression of their perceived contested and precarious belonging: the ordinariness of racism in the UK, the mistrust in the durability of the boundaries of inclusion drawn by the British state, and a heightened alertness for fear of escalating racist and homophobic violence.
Expression of Stress-Mediating Genes is Increased in Term Placentas of Women with Chronic Self-Perceived Anxiety and Depression
Authors: Martinez Cristina A. , Marteinsdottir Ina , Josefsson Ann , Sydsjö Gunilla , Theodorsson Elvar , Rodriguez-Martinez Heriberto
Published: August 2020

Anxiety, chronical stress, and depression during pregnancy are considered to affect the offspring, presumably through placental dysregulation. We have studied the term placentae of pregnancies clinically monitored with the Beck’s Anxiety Inventory (BAI) and Edinburgh Postnatal Depression Scale (EPDS). A cutoff threshold for BAI/EPDS of 10 classed patients into an Index group (>10, n = 23) and a Control group (<10, n = 23). Cortisol concentrations in hair (HCC) were periodically monitored throughout pregnancy and delivery. Expression differences of main glucocorticoid pathway genes, i.e., corticotropin-releasing hormone (CRH), 11β-hydroxysteroid dehydrogenase (HSD11B2), glucocorticoid receptor (NR3C1), as well as other key stress biomarkers (Arginine Vasopressin, AVP and O-GlcNAc transferase, OGT) were explored in medial placentae using real-time qPCR and Western blotting. Moreover, gene expression changes were considered for their association with HCC, offspring, gender, and birthweight. A significant dysregulation of gene expression for CRH, AVP, and HSD11B2 genes was seen in the Index group, compared to controls, while OGT and NR3C1 expression remained similar between groups. Placental gene expression of the stress-modulating enzyme 11β-hydroxysteroid dehydrogenase (HSD11B2) was related to both hair cortisol levels (Rho = 0.54; p < 0.01) and the sex of the newborn in pregnancies perceived as stressful (Index, p < 0.05). Gene expression of CRH correlated with both AVP (Rho = 0.79; p < 0.001) and HSD11B2 (Rho = 0.45; p < 0.03), and also between AVP with both HSD11B2 (Rho = 0.6; p < 0.005) and NR3C1 (Rho = 0.56; p < 0.03) in the Control group but not in the Index group; suggesting a possible loss of interaction in the mechanisms of action of these genes under stress circumstances during pregnancy.
Effect of Mutations on mRNA and Globin Stability: The Cases of Hb Bernalda/Groene Hart and Hb Southern Italy
Authors: Cardiero Giovanna , Musollino Gennaro , Friscia Maria Grazia , Testa Rosario , Virruso Lucrezia , Di Girgenti Caterina , Caldora Mercedes , Colella Bisogno Rosario , Gaudiano Carlo , Manco Giuseppe , Lacerra Giuseppina
Published: August 2020

We identified two unstable variants in the third exon of α-globin genes: Hb Bernalda/Groene Hart (HBA1:c.358C>T), and Hb Caserta (HBA2:c.79G>A) in cis to Hb Sun Prairie (HBA2:c.391G>C), also named Hb Southern Italy. These mutations occurred in the H helix of the α-globin that is involved in heme contacting, specific recognition of α-hemoglobin-stabilizing protein (AHSP), and α1β1 interactions. The carriers showed α-thalassemia phenotype, but one also jaundice and cholelithiasis. Molecular identification of clusters of families in Southern Italy encouraged molecular characterization of mRNA, globin chain analyses, molecular modeling studies, and comparison with globin variants to understand the mechanisms causing the α-thalassemia phenotype. A normal amount of Hb Bernalda/Groene Hart mRNA were found, and molecular modeling highlighted additional H bonds with AHSP. For Hb Southern Italy, showing an unexpected α/β biosynthetic ratio typical of the β-thalassemia type, two different molecular mechanisms were shown: Reduction of the variant mRNA, likely due to the No-Go Decay for the presence of unused triplet ACG at cod 26, and protein instability due to the impairment of AHSP interaction. The UDP glucuronosyltransferase 1A (UGT1A1) genotyping was conclusive in the case of jaundice and cholelithiasis. Multiple approaches are needed to properly identify the mechanisms leading to unstable variants and the effect of a mutation.
InSAR Time-Series Analysis for Monitoring Ground Displacement Trends in the Western Hellenic Arc: The Kythira Island, Greece
Authors: Alatza Stavroula , Papoutsis Ioannis , Paradissis Demitris , Kontoes Charalampos , Papadopoulos Gerassimos A. , Raptakis Costas
Published: August 2020

Kythira Island is situated at the western Hellenic Arc, which is a region of very high seismicity and tectonic activity. On 8 January 2006, a large seismic event of Mw = 6.7 occurred close to Kythira, in association with the Hellenic subduction zone. We present an extensive multi-temporal interferometry study of ground displacements in Kythira Island exploiting SAR data of a decade, from 2003 to 2009, and from 2015 to 2019. The line-of-sight displacement field for the 2003–2009 time period presents small-scale displacements that do not exceed −3 mm/y, identified to the East of Kythira’s airport, and 3 mm/y in the central part of the island. We exploit then Sentinel-1 data from 2015 to 2019, of both descending and ascending Sentinel-1 SAR imaging modes to decompose the line-of-sight measured deformation to vertical and east–west motion components. Higher vertical displacement rates characterize the central part of the island, with a maximum value of 5 mm/y. This short-term uplift rate exceeds by an order of magnitude the long-term geologic uplift rate of ~0.13 mm/y found in the island during the Quaternary. We discuss possible regional geophysical explanations for this discrepancy. Strike-slip components are detected in the N–E coast of the Potamos village, where horizontal displacements occur, with an east-ward trend and a maximum value of −3 mm/y. These insights are valuable input for the systematic monitoring of this high seismic risk island and the dynamic assessment of its hazard potential.
Analysis of Health-Related Behaviors of Adult Korean Women at Normal BMI with Different Body Image Perceptions: Results from the 2013–2017 Korea National Health and Nutrition Examination Survey (KNHNES)
Authors: Park Seyeon , Shin Jieun , Baek Seunghui
Published: August 2020

The tendency of misperceiving one’s body image was found to be higher among those at normal body mass index (BMI). Thus, the present study aims to provide basic data to seek solutions for ideal physical activities and right body image perception by comparing health-related behaviors of women at normal BMI. Among the 39,225 respondents from the Korea National Health and Nutrition Examination Survey (KNHNES) conducted from 2013 to 2017, 10,798 adult women with World Health Organization (WHO) BMI Classifications of 18.5 ≤ BMI < 25 (Asia-Pacific) were considered, from which pregnant and breast-feeding women and women whose body image perception was not identified were excluded, leading to a total of 9288 women. Data were analyzed utilizing SAS ver. 9.4 for frequency analysis, cross tabulation, GLM (generalized linear model), and logistic regression analysis with complex samples design, in conformity with the guidelines of the KNHNES. The results showed that approximately most (87.6%) of adult Korean women misperceived their body image. Misperception of body image was related to inappropriate health-related behaviors such as smoking, insufficient sleeping, and excessive body weight management; those who had underestimated their body image (≤64 odds ratio (OR) (0.718 (confidence interval (C.I.) 0.594–0.866))) carried out fewer health-related behaviors, while women aged 65 or above engaged in more health-related behaviors when they perceived themselves as obese (OR 1.683 (C.I. 1.260–2.248; overestimation)). To sum up, it was found that lack of health management, inappropriate body weight control, and health-related behaviors are related to body image misperception compared with real BMI. As such, it is necessary to have educational programs to encourage building proper perception of one’s body image and body weight, and to carry out health-related behaviors.
Wellbeing at Work before and during the SARS-COV-2 Pandemic: A Brazilian Nationwide Study among Dietitians
Authors: Matos Raquel Adjafre da Costa , Akutsu Rita de Cássia Coelho de Almeida , Zandonadi Renata Puppin , Rocha Ada , Botelho Raquel Braz Assunção
Published: August 2020

This study aimed to evaluate the perceptions of dietitians’ wellbeing at work before and during the SARS-COV-2 pandemic in Brazil. This cross-sectional study was performed using a previously validated instrument to investigate the wellbeing of dietitians at work in Brazil. The questionnaire on the wellbeing of dietitians was composed of 25 items (with a 5-point scale), characteristics, and questions about the SARS-COV-2 period. The application was carried out with GoogleForms® tool from 26 May to 7 June 2020. The weblink to access the research was sent via email, messaging apps, and social networks. Volunteers were recruited nationwide with the help of the Brazilian Dietitians Councils, support groups, as well as media outreach to reach as many dietitians as possible. Volunteers received, along with the research link, the invitation to participate, as well as the consent form. A representative sample of 1359 dietitians from all the Brazilian regions answered the questionnaire—mostly female (92.5%), Catholic (52.9%), from 25 to 39 years old (58.4%), with a partner (63.8%), and with no children (58%). Most of the participants continue working during the pandemic period (83.8%), but they did not have SARS-COV-2 (96%), nor did their family members (80.7%). The wellbeing at work before SARS-COV-2 was 3.88 ± 0.71, statistically different (p < 0.05) from during the pandemic, with the wellbeing of 3.71 ± 0.78. Wellbeing at work was higher before the pandemic for all the analyzed variables. Analyzing variables separately before and during the pandemic, dietitians with partners, children and a Ph.D. presented higher scores for wellbeing at work. Professionals receiving more than five times the minimum wage have higher scores. During the pandemic, better wellbeing was observed for dietitians working remotely.
Combatting Intimate Partner Violence: Representations of Social and Healthcare Personnel Working with Gender-Based Violence Interventions
Authors: Autiero Marcella , Procentese Fortuna , Carnevale Stefania , Arcidiacono Caterina , Di Napoli Immacolata
Published: August 2020

Intimate partner violence (IPV) has been declared a global epidemic by the World Health Organization. Although the attention paid to both the perpetrators and victims of gender-based violence has increased, scientific research is still lacking in regard to the representations of operators involved in interventions and management. Therefore, the following study explores how the representations of operators affect how gender violence can be managed and combatted through an ecological approach to this phenomenon, in addition to highlighting the roles of organizational-level services and their cultural and symbolic substrates. In total, 35 health and social professionals were interviewed and textual materials were analyzed by thematic analysis. The evidence suggests that services contrasting gender-based violence utilize different representations and management approaches. The authors hope that these differences can become a resource, rather than a limitation, when combatting gender-based violence through the construction of more integrated networks and a greater dialogue among different services, in order to make interventions designed to combat gender-based violence more effective.
Physical Activity Promotes Health and Reduces Cardiovascular Mortality in Depressed Populations: A Literature Overview
Authors: Belvederi Murri Martino , Folesani Federica , Zerbinati Luigi , Nanni Maria Giulia , Ounalli Heifa , Caruso Rosangela , Grassi Luigi
Published: August 2020

Major depression is associated with premature mortality, largely explained by heightened cardiovascular burden. This narrative review summarizes secondary literature (i.e., reviews and meta-analyses) on this topic, considering physical exercise as a potential tool to counteract this alarming phenomenon. Compared to healthy controls, individuals with depression consistently present heightened cardiovascular risk, including “classical” risk factors and dysregulation of pertinent homeostatic systems (immune system, hypothalamic–pituitary–adrenal axis and autonomic nervous system). Ultimately, both genetic background and behavioral abnormalities contribute to explain the link between depression and cardiovascular mortality. Physical inactivity is particularly common in depressed populations and may represent an elective therapeutic target to address premature mortality. Exercise-based interventions, in fact, have proven effective reducing cardiovascular risk and mortality through different mechanisms, although evidence still needs to be replicated in depressed populations. Notably, exercise also directly improves depressive symptoms. Despite its potential, however, exercise remains under-prescribed to depressed individuals. Public health may be the ideal setting to develop and disseminate initiatives that promote the prescription and delivery of exercise-based interventions, with a particular focus on their cost-effectiveness.
A Comparative Study of Participation in Physical Education Classes among 170,347 Adolescents from 54 Low-, Middle-, and High-Income Countries
Authors: Martins João , Marques Adilson , Peralta Miguel , Henriques-Neto Duarte , Costa João , Onofre Marcos , González Valeiro Miguel
Published: August 2020

Given the need for comparative research on the participation of adolescents in physical education (PE) classes as a privileged space for physical activity (PA) promotion, this study sought to estimate, analyse and compare the prevalence of participation in PE classes among adolescents from 54 countries and to examine sex, age, country income and world regions disparities. Data from the Global Students Health Survey (2010–2015) were used, comprising 170,347 adolescents (90,305 girls, aged 13–17 years) from nationally representative samples of 54 countries—of which 7 are low-income, 23 lower-middle-income, 14 upper-middle-income and 10 high-income—and six world regions. The weighted percentages of adolescents participating in PE classes (never, 1–2 days/week, 3–4 days/week, 5 or more days/week) were estimated along 95% confidence intervals and compared across sex, age, country income, region, and country. Most adolescents reported to participate in PE on 1–2 days/week (55.2%), but almost 20% of adolescents reported never participating in PE. Girls, compared to boys, presented a lower prevalence for participating ≥5 days/week (girls 16.8%, boys 20.0%). The prevalence of participating in PE on ≥3 days/week was higher among adolescents aged 13–14 years when compared to adolescents aged 15–17 years (boys: 30.9% vs. 24.6%; girls: 26.1% vs. 18.2%). Concerning the countries’ income, the prevalence of never participating in PE was higher in high-income countries, and participating on ≥3 days/week was higher in low-income countries, but further research is recommended. The findings suggest that national, regional and worldwide data highlight the importance of improving participation in PE, particularly for girls and older adolescents. An improved and continued monitoring of PE policies and their actual implementation is needed worldwide.
Burnout Syndrome in Police Officers and Its Relationship with Physical and Leisure Activities
Authors: García-Rivera Blanca Rosa , Olguín-Tiznado Jesús Everardo , Aranibar Mónica Fernanda , Ramírez-Barón María Concepción , Camargo-Wilson Claudia , López-Barreras Juan Andrés , García-Alcaraz Jorge Luis
Published: August 2020

No previous studies in Mexico have been found that jointly analyze physical and leisure activities as variables related to mental health in police officers. This paper presents research on burnout in Mexican Police officers. The question it answers is: is there any association of burnout with physical and leisure activities and personal profile? A total of 276 police officers (87% men and 13% women) participated. To obtain information, the Spanish Burnout Inventory and the Operational Police Stress questionnaires were used. A cross sectional study design was utilized with tests of validity and reliability, goodness of fit, analysis of variance (ANOVA), and analysis of k-means clusters. Results showed that a high number of policemen had high prevalence of burnout and a high level of mental exhaustion, and that exercise was positively and significantly related to lower burnout risk. Men showed higher risk than women. Results should be considered to improve interventions and occupational health practices in the police force. This paper improves understanding of burnout among policemen and the importance of exercise and leisure activities to alleviate burnout.
Beliefs about the Harmfulness of Heated Tobacco Products Compared with Combustible Cigarettes and Their Effectiveness for Smoking Cessation among Korean Adults
Authors: Kim Seung Hee , Kang Seo Young , Cho Hong-Jun
Published: August 2020

Heated tobacco products (HTPs) have been widely used in Korea since their introduction in 2017. In this study, we investigated the perceptions of their relative harmfulness and smoking cessation effects. We performed an online survey in 7000 Koreans in 2018 (2300 males and 4700 females aged 20–69 years) by matching their age, sex, and provincial distribution. To investigate the factors causing HTPs to be perceived as less harmful than combustible cigarettes (CCs) and helpful for smoking cessation, we used multivariable logistic regression analyses. HTPs were less harmful than CCs in 16.8% of participants, particularly among HTP-only users and dual and triple users of HTPs, electronic cigarettes (ECs), or CCs than among CC-only users, those who were aged ≤ 34 years, males, and those with higher incomes. HTPs were reportedly helpful for smoking cessation in 11.2% of participants. Similar perceptions were more likely among HTP-only users, as well as dual and triple users than among CC-only users and adults with higher education/incomes. Although Korean adults generally had negative perceptions of the harmfulness and smoking cessation effects of HTPs compared with CCs, dual and triple users were more likely to have positive perceptions. Monitoring the use of multiple tobacco products and HTPs is a new challenge for Korean policymakers.
Is Achieving the Guidelines of Four Forms of Physical Activity Associated with Less Self-Reported Health Complaints? Cross-Sectional Study of Undergraduates at the University of Turku, Finland
Authors: El Ansari Walid , Salam Abdul
Published: August 2020

Very little research has assessed the physical activity (PA) of university students in in Finland, and their associations with self-reported health complaints (HCs), whilst simultaneously accounting for a range of other potential confounders. Students at the University of Turku (1177) completed an online health and wellbeing questionnaire that assessed 22 physical and somatic HCs, and students’ achievement of the international guidelines of four forms of PA (moderate, vigorous, moderate-to-vigorous and muscle strengthening PA; MPA, VPA, MVPA, MSPA respectively). We also explored the associations between HCs and PA, controlling for sociodemographic and health confounders (age, sex, year of study, marital status, accommodation during semesters, health awareness). Factor analysis reduced the HCs into three factors (psychological, pains/aches, circulatory/breathing). Bivariate relationships (no controlling for confounders) between these 3 factors and four forms of PA guideline achievement showed significant effects of achieving the PA guidelines against various groups of HCs, where more strenuous PA was associated with significantly less HCs in a step-ladder pattern. Multiple regression analyses (controlling for confounders) showed that achievement of PA guidelines was significantly independently associated with self-reported HCs scores in most cases. Psychological HCs were negatively associated with achieving any type of PA; pains/aches were negatively associated with achieving two types of PA or with achieving MSPA guidelines; and circulatory/breathing HCs were negatively associated with achieving the VPA guidelines only. This is the first study in Finland to examine such relationships, and highlights the critical role of PA for the health of these young adults. Programs and policies to strengthen and improve the PA of university students would be beneficial, recognizing the benefits of instilling life-long PA habits among this group of young adults.
Mechanical Debridement with Antibiotics in the Treatment of Chronic Periodontitis: Effect on Systemic Biomarkers―A Systematic Review
Authors: Munasur Sudhir L. , Turawa Eunice B. , Chikte Usuf M.E. , Musekiwa Alfred
Published: August 2020

In this systematic review, we assessed the effectiveness of systemic antibiotics as an adjunctive therapy to mechanical debridement in improving inflammatory systemic biomarkers, as compared to mechanical debridement alone, among adults with chronic periodontitis. We searched relevant electronic databases for eligible randomized controlled trials. Two review authors independently screened, extracted data, and assessed risk of bias. We conducted meta-analysis, assessed heterogeneity, and assessed certainty of evidence using GRADEPro software. We included 19 studies (n = 1350 participants), representing 18 randomized controlled trials and found very little or no impact of antibiotics on inflammatory biomarkers. A meta-analysis of eight studies demonstrated a mean reduction of 0.26 mm in the periodontal pockets at three months (mean difference [MD] −0.26, 95%CI: −0.36 to −0.17, n = 372 participants, moderate certainty of evidence) in favor of the antibiotics. However, results from five studies reporting clinical attachment level (mm) yielded little or no difference at three months (MD −0.16, 95% CI: −0.35 to 0.03, n = 217 participants) between antibiotic and placebo groups. There is little or no evidence that adjunctive systemic antibiotics therapy improves inflammatory systemic biomarkers, compared to mechanical debridement alone, among adults with chronic periodontitis.
Does the Act of Copulation per se, without Considering Seminal Deposition, Change the Expression of Genes in the Porcine Female Genital Tract?
Authors: Alvarez-Rodriguez Manuel , Martinez Cristina A. , Wright Dominic , Rodriguez-Martinez Heriberto
Published: August 2020

Semen—through its specific sperm and seminal plasma (SP) constituents—induces changes of gene expression in the internal genital tract of pigs, particularly in the functional sperm reservoir at the utero-tubal junction (UTJ). Although seminal effects are similarly elicited by artificial insemination (AI), major changes in gene expression are registered after natural mating, a fact suggesting the act of copulation induces per se changes in genes that AI does not affect. The present study explored which pathways were solely influenced by copulation, affecting the differential expression of genes (DEGs) of the pre/peri-ovulatory genital tract (cervix, distal uterus, proximal uterus and UTJ) of estrus sows, 24 h after various procedures were performed to compare natural mating with AI of semen (control 1), sperm-free SP harvested from the sperm-peak fraction (control 2), sperm-free SP harvested from the whole ejaculate (control 3) or saline-extender BTS (control 4), using a microarray chip (GeneChip® porcine gene 1.0 st array). Genes related to neuroendocrine responses (ADRA1, ADRA2, GABRB2, CACNB2), smooth muscle contractility (WNT7A), angiogenesis and vascular remodeling (poFUT1, NTN4) were, among others, overrepresented with distal and proximal uterine segments exhibiting the highest number of DEGs. The findings provide novel evidence that relevant transcriptomic changes in the porcine female reproductive tract occur in direct response to the specific act of copulation, being semen-independent.
Benefits of Polydopamine as Particle/Matrix Interface in Polylactide/PD-BaSO4 Scaffolds
Authors: Sadaba Naroa , Larrañaga Aitor , Orpella-Aceret Gemma , Bettencourt Ana F. , Martin Victor , Biggs Manus , Ribeiro Isabel A. C. , Ugartemendia Jone M. , Sarasua Jose-Ramon , Zuza Ester
Published: August 2020

This work reports the versatility of polydopamine (PD) when applied as a particle coating in a composite of polylactide (PLA). Polydopamine was observed to increase the particle–matrix interface strength and facilitate the adsorption of drugs to the material surface. Here, barium sulfate radiopaque particles were functionalized with polydopamine and integrated into a polylactide matrix, leading to the formulation of a biodegradable and X-ray opaque material with enhanced mechanical properties. Polydopamine functionalized barium sulfate particles also facilitated the adsorption and release of the antibiotic levofloxacin. Analysis of the antibacterial capacity of these composites and the metabolic activity and proliferation of human dermal fibroblasts in vitro demonstrated that these materials are non-cytotoxic and can be 3D printed to formulate complex biocompatible materials for bone fixation devices.
Targeting on Gut Microbiota-Derived Metabolite Trimethylamine to Protect Adult Male Rat Offspring against Hypertension Programmed by Combined Maternal High-Fructose Intake and Dioxin Exposure
Authors: Hsu Chien-Ning , Chan Julie Y. H. , Yu Hong-Ren , Lee Wei-Chia , Wu Kay L. H. , Chang-Chien Guo-Ping , Lin Sufan , Hou Chih-Yao , Tain You-Lin
Published: August 2020

Gut microbiota-dependent metabolites, in particular trimethylamine (TMA), are linked to hypertension. Maternal 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) exposure or consumption of food high in fructose (HFR) can induce hypertension in adult offspring. We examined whether 3,3-maternal dimethyl-1-butanol (DMB, an inhibitor of TMA formation) therapy can protect adult offspring against hypertension arising from combined HFR and TCDD exposure. Pregnant Sprague–Dawley rats received regular chow or chow supplemented with fructose (60% diet by weight) throughout pregnancy and lactation. Additionally, the pregnant dams received TCDD (200 ng/kg BW orally) or a corn oil vehicle on days 14 and 21 of gestation, and days 7 and 14 after birth. Some mother rats received 1% DMB in their drinking water throughout pregnancy and lactation. Six groups of male offspring were studied (n = 8 for each group): regular chow (CV), high-fructose diet (HFR), regular diet+TCDD exposure (CT), HFR+TCDD exposure (HRT), high-fructose diet+DMB treatment (HRD), and HFR+TCDD+DMB treatment (HRTD). Our data showed that TCDD exacerbates HFR-induced elevation of blood pressure in male adult offspring, which was prevented by maternal DMB administration. We observed that different maternal insults induced distinct enterotypes in adult offspring. The beneficial effects of DMB are related to alterations of gut microbiota, the increase in nitric oxide (NO) bioavailability, the balance of the renin-angiotensin system, and antagonization of aryl hydrocarbon receptor (AHR) signaling. Our findings cast new light on the role of early intervention targeting of the gut microbiota-dependent metabolite TMA, which may allow us to prevent the development of hypertension programmed by maternal excessive fructose intake and environmental dioxin exposure.
Oxygenated Water Inhibits Adipogenesis and Attenuates Hepatic Steatosis in High-Fat Diet-Induced Obese Mice
Authors: Cheng Yuh-Jen , Liu Chao-Chi , Chu Fang-Yeh , Yang Ching-Ping , Hsiao Chiao-Wan , Chuang Cheng-Wei , Shiau Ming-Yuh , Lee Hsueh-Te , Tsai Jen-Ning , Chang Yih-Hsin
Published: August 2020

The expansion of adipose tissue mass is the primary characteristic of the process of becoming obesity, which causes chronic adipose inflammation and is closely associated with type 2 diabetes mellitus (T2DM). Adipocyte hypertrophy restricts oxygen availability, leading to microenvironmental hypoxia and adipose dysfunction. This study aimed at investigating the effects of oxygenated water (OW) on adipocyte differentiation (adipogenesis) and the metabolic function of mature adipocytes. The effects of OW on adipogenesis and the metabolic function of mature adipocytes were examined. Meanwhile, the in vivo metabolic effects of long-term OW consumption on diet-induced obesity (DIO) mice were investigated. OW inhibited adipogenesis and lipid accumulation through down-regulating critical adipogenic transcription factors and lipogenic enzymes. While body weight, blood and adipose parameters were not significantly improved by long-term OW consumption, transient circulatory triglyceride-lowering and glucose tolerance-improving effects were identified. Notably, hepatic lipid contents were significantly reduced, indicating that the DIO-induced hepatic steatosis was attenuated, despite no improvements in fibrosis and lipid contents in adipose tissue being observed in the OW-drinking DIO mice. The study provides evidence regarding OW’s effects on adipogenesis and mature adipocytes, and the corresponding molecular mechanisms. OW exhibits transient triglyceride-lowering and glucose tolerance-improving activity as well as hepatic steatosis-attenuating functions.
Control of Innate Immunity by Sialic Acids in the Nervous Tissue
Authors: Liao Huan , Klaus Christine , Neumann Harald
Published: August 2020

Sialic acids (Sias) are the most abundant terminal sugar residues of glycoproteins and glycolipids on the surface of mammalian cells. The nervous tissue is the organ with the highest expression level of Sias. The ‘sialylation’ of glycoconjugates is performed via sialyltransferases, whereas ‘desialylation’ is done by sialidases or is a possible consequence of oxidative damage. Sialic acid residues on the neural cell surfaces inhibit complement and microglial activation, as well as phagocytosis of the underlying structures, via binding to (i) complement factor H (CFH) or (ii) sialic acid-binding immunoglobulin-like lectin (SIGLEC) receptors. In contrast, activated microglial cells show sialidase activity that desialylates both microglia and neurons, and further stimulates innate immunity via microglia and complement activation. The desialylation conveys neurons to become susceptible to phagocytosis, as well as triggers a microglial phagocytosis-associated oxidative burst and inflammation. Dysfunctions of the ‘Sia–SIGLEC’ and/or ‘Sia–complement’ axes often lead to neurological diseases. Thus, Sias on glycoconjugates of the intact glycocalyx and its desialylation are major regulators of neuroinflammation.
Targeting of CD163+ Macrophages in Inflammatory and Malignant Diseases
Authors: Skytthe Maria K. , Graversen Jonas Heilskov , Moestrup Søren K.
Published: August 2020

The macrophage is a key cell in the pro- and anti-inflammatory response including that of the inflammatory microenvironment of malignant tumors. Much current drug development in chronic inflammatory diseases and cancer therefore focuses on the macrophage as a target for immunotherapy. However, this strategy is complicated by the pleiotropic phenotype of the macrophage that is highly responsive to its microenvironment. The plasticity leads to numerous types of macrophages with rather different and, to some extent, opposing functionalities, as evident by the existence of macrophages with either stimulating or down-regulating effect on inflammation and tumor growth. The phenotypes are characterized by different surface markers and the present review describes recent progress in drug-targeting of the surface marker CD163 expressed in a subpopulation of macrophages. CD163 is an abundant endocytic receptor for multiple ligands, quantitatively important being the haptoglobin-hemoglobin complex. The microenvironment of inflammation and tumorigenesis is particular rich in CD163+ macrophages. The use of antibodies for directing anti-inflammatory (e.g., glucocorticoids) or tumoricidal (e.g., doxorubicin) drugs to CD163+ macrophages in animal models of inflammation and cancer has demonstrated a high efficacy of the conjugate drugs. This macrophage-targeting approach has a low toxicity profile that may highly improve the therapeutic window of many current drugs and drug candidates.