bluuebunny/crossref_metadata_2025 huggingface dataset:
Clinicians’ prescribing pattern, rate of patients’ medication adherence and its determinants among adult hypertensive patients at Jimma University Medical Center: Prospective cohort study
Authors: Simegn Bekalu Kebede , Chelkeba Legese , Alamirew Bekalu Dessie
Published: November 2021

Background

Many studies conducted in the past focused on patients’ sociodemographic factors and medical profiles to identify the determinants of suboptimal blood pressure control. However, prescribing patterns and clinicians’ adherence to guidelines are also important factors affecting the rate of blood pressure control. Therefore, this study aimed to determine clinicians’ prescribing patterns, patients’ medication adherence, and its determinants among hypertensive patients at Jimma University Medical Center.

Methods

A general prospective cohort study was conducted among hypertensive patients who had regular follow-up at Jimma university ambulatory cardiac clinic from March 20, 2018, to June 20, 2018. Patients’ specific data was collected with a face-to-face interview and from their medical charts. Clinicians’ related data were collected through a self-administered questionnaire. Data were analyzed using SPSS version 21.0. Bivariate and multivariable logistic regression analyses were done to identify key independent variables influencing patients’ adherence. P-Values of less than 0.05 were considered statically significant.

Results

From the total of 416 patients, 237(57.0%) of them were males with a mean age of 56.50 ± 11.96 years. Angiotensin-converting enzyme inhibitors were the most frequently prescribed class of antihypertensives, accounting for 261(63.7%) prescriptions. Combination therapy was used by the majority of patients, with 275 (66.1%) patients receiving two or more antihypertensive drugs. Patients’ medication adherence was 46.6%, while clinicians’ guideline adherence was 44.2%. Patients with merchant occupation (P = 0.020), physical inactivity (P = 0.033), and diabetes mellitus co-morbidity (P = 0.008) were significantly associated with a higher rate of medication non-adherence.

Conclusion

The rate of medication adherence was poor among hypertensive patients. Physicians were not-adherent to standard treatment guideline. The most commonly prescribed class of drugs were angiotensin-converting enzyme inhibitors. Effective education should be given to patients to improve medication adherence. Prescribers should be trained on treatment guidelines regularly to keep them up-to-date with current trends of hypertension treatment and for better treatment outcomes.
Acute effects of the different relaxation periods during passive intermittent static stretching on arterial stiffness
Authors: Higaki Yuya , Yamato Yosuke , Fujie Shumpei , Inoue Kenichiro , Shimomura Mio , Kato Shosaku , Horii Naoki , Ogoh Shigehiko , Iemitsu Motoyuki
Published: November 2021

To clarify whether the relaxation period during stretching affects the degree of elevated shear rate and the degree of reduction of arterial stiffness, we examined relaxation duration to build an adequate stretching protocol. In Experiment 1, the changes in cardiac output, the shear rate in the posterior tibial artery, and blood volume in the calf muscle were measured during recovery (0–60 s) from a single bout of one-legged passive calf stretching in 12 healthy young men. In Experiment 2, the effects of different relaxation periods (5-, 10-, 20-, and 60-s) of passive one-legged intermittent calf stretching (30-s × 6 sets) on the femoral-ankle pulse wave velocity (faPWV) as an index of peripheral arterial stiffness were identified in 17 healthy young men. As a result, the stretched leg’s shear rate significantly increased from 0 to 10th s after stretching. The muscle blood volume in the stretched leg significantly reduced during stretching, and then significantly increased during the recovery period after stretching; however, cardiac output remained unchanged during stretching and recovery. Additionally, the reduction in faPWV from the pre-stretching value in the stretched leg was significantly larger in the protocol with 10-s and 20-s relaxation periods than that in the non-stretched leg, but this did not differ in the 5-s and 60-s relaxation periods. These findings suggest that the relaxation periods of intermittent static stretching that cause a high transient increase in shear rate (via reperfusion after microvascular compression by the stretched calf muscles) are effective to reduce arterial stiffness.
Differential effects of four intramuscular sedatives on cardiorespiratory stability in juvenile guinea pigs (Cavia porcellus)
Authors: Sixtus Ryan P. , Pacharinsak Cholawat , Gray Clint L. , Berry Mary J. , Dyson Rebecca M.
Published: November 2021

Background

Non-invasive physiological monitoring can induce stress in laboratory animals. Sedation reduces the level of restraint required, thereby improving the validity of physiological signals measured. However, sedatives may alter physiological equilibrium introducing unintended bias and/or, masking the experimental outcomes of interest. We aimed to investigate the cardiorespiratory effects of four short-acting sedatives in juvenile guinea pigs.

Method

12 healthy, 38 (26–46) day-old Dunkin Hartley guinea pigs were included in this blinded, randomised, crossover design study. Animals were sedated by intramuscular injection using pre-established minimum effective doses of either alfaxalone (5 mg/kg), diazepam (5 mg/kg), ketamine (30 mg/kg), or midazolam (2 mg/kg) administered in random order with a minimum washout period of 48 hours between agents. Sedative depth, a composite score comprised of five assessment criteria, was observed every 5-min from dosing until arousal. Physiological monitoring of cardiorespiratory status included measures of heart rate, blood pressure, respiratory rate, and peripheral microvascular perfusion.

Results

Ketamine and alfaxalone were most effective in inducing stable sedation suitable for physiological monitoring, and diazepam less-so. Midazolam was unsuitable due to excessive hypersensitivity. All sedatives significantly increased heart rate above non-sedated control rates (P<0.0001), without altering blood pressure or microvascular perfusion. Alfaxalone and ketamine reduced respiratory rate relative to their control condition (P<0.0001, P = 0.05, respectively), but within normative ranges.

Conclusion

Ketamine and alfaxalone are the most effective sedatives for inducing short duration, stable sedation with minimal cardiorespiratory depression in guinea pigs, while diazepam is less-so. However, alfaxalone is the most appropriate sedative for longitudinal studies requiring multiple physiological timepoints.
Antimicrobial resistance, virulence genes and biofilm formation in Enterococcus species isolated from milk of sheep and goat with subclinical mastitis
Authors: El-Zamkan Mona A. , Mohamed Hams M. A.
Published: November 2021

This study is designed to discuss the antimicrobial resistance, virulence determinants and biofilm formation capacity of Enterococcus spp. isolated from milk of sheep and goat with subclinical mastitis in Qena, Egypt. The obtained isolates were identified by the VITEK2 system and 16S rDNA sequencing as E. faecalis, E. faecium, E. casseliflavus and E. hirae. Overall, E. faecalis and E. faecium were the dominant species recovered from mastitic milk samples. The antimicrobial susceptibility test evidenced multidrug resistance of the isolates against the following antimicrobials: oxacillin (89.2.%), followed by vancomycin (75.7%) and linezolid (70.3%). Also, most of these isolates (73%) could form biofilms. For example, 18.9% of Enterococcus strains formed strong biofilm, whereas 32.4% of isolates formed moderate biofilm and 21.6% of isolates formed weak biofilm. The most prevalent resistance genes found in our isolates were blaZ (54%), vanA (40%), ermB (51.4%), tetM (13.5%) and optrA (10.8%). Moreover, asa1 (37.8%), cylA (42.3%), gelE (78.4%), esp (32.4%), EF3314(48.6%) and ace (75.5%) were the most common virulence genes. A significant correlation was found between biofilm formation, multidrug resistance and virulence genes of the isolates. This study highlights several aspects of virulence and harmfulness of Enterococcus strains isolated from subclinical mastitic milk, which necessitates continuous inspection and monitoring of dairy animals.
Collection of human and environmental data on pesticide use in Europe and Argentina: Field study protocol for the SPRINT project
Authors: Silva Vera , Alaoui Abdallah , Schlünssen Vivi , Vested Anne , Graumans Martien , van Dael Maurice , Trevisan Marco , Suciu Nicoleta , Mol Hans , Beekmann Karsten , Figueiredo Daniel , Harkes Paula , Hofman Jakub , Kandeler Ellen , Abrantes Nelson , Campos Isabel , Martínez María Ángeles , Pereira Joana Luísa , Goossens Dirk , Gandrass Juergen , Debler Freya , Lwanga Esperanza Huerta , Jonker Marlot , van Langevelde Frank , Sorensen Martin T. , Wells Jerry M. , Boekhorst Jos , Huss Anke , Mandrioli Daniele , Sgargi Daria , Nathanail Paul , Nathanail Judith , Tamm Lucius , Fantke Peter , Mark Jennifer , Grovermann Christian , Frelih-Larsen Ana , Herb Irina , Chivers Charlotte-Anne , Mills Jane , Alcon Francisco , Contreras Josefina , Baldi Isabelle , Pasković Igor , Matjaz Glavan , Norgaard Trine , Aparicio Virginia , Ritsema Coen J. , Geissen Violette , Scheepers Paul T. J.
Published: November 2021

Current farm systems rely on the use of Plant Protection Products (PPP) to secure high productivity and control threats to the quality of the crops. However, PPP use may have considerable impacts on human health and the environment. A study protocol is presented aiming to determine the occurrence and levels of PPP residues in plants (crops), animals (livestock), humans and other non-target species (ecosystem representatives) for exposure modelling and impact assessment. To achieve this, we designed a cross-sectional study to compare conventional and organic farm systems across Europe. Environmental and biological samples were/are being/will be collected during the 2021 growing season, at 10 case study sites in Europe covering a range of climate zones and crops. An additional study site in Argentina will inform the impact of PPP use on growing soybean which is an important European protein-source in animal feed. We will study the impact of PPP mixtures using an integrated risk assessment methodology. The fate of PPP in environmental media (soil, water and air) and in the homes of farmers will be monitored. This will be complemented by biomonitoring to estimate PPP uptake by humans and farm animals (cow, goat, sheep and chicken), and by collection of samples from non-target species (earthworms, fish, aquatic and terrestrial macroinvertebrates, bats, and farm cats). We will use data on PPP residues in environmental and biological matrices to estimate exposures by modelling. These exposure estimates together with health and toxicity data will be used to predict the impact of PPP use on environment, plant, animal and human health. The outcome of this study will then be integrated with socio-economic information leading to an overall assessment used to identify transition pathways towards more sustainable plant protection and inform decision makers, practitioners and other stakeholders regarding farming practices and land use policy.
Modeling and performance analysis of shuttle-based compact storage systems under parallel processing policy
Authors: Deng Lei , Chen Lei , Zhao Jingjie , Wang Ruimei
Published: November 2021

Short response time for order processing is important for modern warehouses, which can be potentially achieved by adopting appropriate processing policy. The parallel processing policy have advantages in improving performance of many autonomous storage and retrieval systems. However, researchers tend to assume a sequential processing policy managing the movement of independent resources in shuttle-based compact storage systems. This paper models and analyses a single-tier of specialized shuttle-based compact storage systems under parallel processing policy. The system is modeled as a semi-open queueing network with class switching and the parallel movement of shuttles and the transfer car is modeled using a fork-join queueing network. The analytical model is validated against simulations and the results show our model can accurately estimate the system performance. Numerical experiments and a real case are carried out to compare the performance of parallel and sequential processing policies. The results suggest a critical transaction arrival rate and depth/width ratio, below which the sequential processing policy outperforms the parallel processing policy. However, the advantage of sequential processing policy is decreasing with the increasing of shuttle number, transaction arrival rate and depth/width ratio. The results also suggest an optimal depth/width ratio with a value of 1.75 for minimizing the expected throughput time in the real system. Given the current system configurations, the parallel processing policy should be considered when the number of shuttles is larger than 2 or the transaction arrival rate is larger than 24 per hour.
Influence of UTCP on the employment of female workers and the supply of labor force
Authors: Li Mingming , Wang Jiayun
Published: November 2021

In recent years, China has introduced the Universal Two-Child Policy (UTCP), which means that a couple can have two children. The implementation of this policy may affect female employment. Based on this background, this work aims to the impact of UTCP on the number and employment of Chinese women workers, and find out the countermeasures for the adverse impact of the policy. Firstly, the role of the Propensity Score Matching-Differences in Differences model is introduced, and the average and dynamic effects of UTCP on women’s employment are discussed by using the Propensity Score Matching model. Secondly, the survey data on issues related to female employment after the implementation of UTCP from 2016 to 2020 is analyzed. Finally, a conclusion is drawn according to the survey data. The results demonstrate that the implementation of UTCP widens the income gap between men and women. Meanwhile, the younger the couple, the greater the income gap. Besides, the unemployment rate changes slightly after the introduction of the policy. As the growth rate of female income is significantly lower than that of men of the same age, UTCP has little impact on the employment of Chinese female workers, but has a great impact on the quality of employment. Among all the respondents, the proportion of employed men is higher than employed women, which is about 64% ~ 65%. However, it is still unknown whether age, education, family characteristics, nationality, occupations, and economic development of the province have a certain impact on female income, which is worth noting by follow-up research. On the whole, the full liberalization of the second child has little impact on the employment of female workers in China, but has a great impact on the quality of employment. The present work lays a foundation for the study of the impact of UTCT on female employment in future, and offers a certain reference for the further study of the impact of the policy on employment in the future.
Distribution of subcutaneous and intermuscular fatty tissue of the mid-thigh measured by MRI—A putative indicator of serum adiponectin level and individual factors of cardio-metabolic risk
Authors: Hassler Eva Maria , Deutschmann Hannes , Almer Gunter , Renner Wilfried , Mangge Harald , Herrmann Markus , Leber Stefan , Michenthaler Manuela , Staszewski Alexander , Gunzer Felix , Partl Richard , Reishofer Gernot
Published: November 2021

Obesity and metabolic syndrome (MetS) are associated with hypoadiponectinemia. On the contrary, studies revealed correlations between the amount of subcutaneous adipose tissue (SAT) and higher serum adiponectin levels. Furthermore, independent association of intermuscular adipose tissue (IMAT) deposit in the thigh with cardiometabolic risk factors (including total blood cholesterol, low-density lipoprotein (LDL), and triglycerides), and decreased insulin sensitivity, as MetS components, are sufficiently described. The combined relationship of thigh IMAT and SAT with serum adiponectin, leptin levels, and cardiometabolic risk factors have not been investigated till date. Since both SAT and IMAT play a role in fat metabolism, we hypothesized that the distribution pattern of SAT and IMAT in the mid-thigh might be related to adiponectin, leptin levels, and serum lipid parameters. We performed adipose tissue quantification using magnetic resonance imaging (MRI) of the mid-thigh in 156 healthy volunteers (78 male/78 female). Laboratory measurements of lipid panel, serum adiponectin, and leptin levels were conducted. Total serum adiponectin level showed a significant correlation with the percentage of SAT of the total thigh adipose tissue (SAT/ (IMAT+SAT)) for the whole study population and in sex-specific analysis. Additionally, SAT/(IMAT+SAT) was negatively correlated with known cardiometabolic risk factors such as elevated total blood cholesterol, LDL, and triglycerides; but positively correlated with serum high-density lipoprotein. In multiple linear regression analysis, (SAT/(IMAT+SAT)) was the most strongly associated variable with adiponectin. Interestingly, leptin levels did not show a significant correlation with this ratio. Adipose tissue distribution in the mid-thigh is not only associated to serum adiponectin levels, independent of sex. This proposed quantitative parameter for adipose tissue distribution could be an indicator for individual factors of a person`s cardiometabolic risk and serve as additional non-invasive imaging marker to ensure the success of lifestyle interventions.
Can vaccination roll-out be more equitable if population risk is taken into account?
Authors: Sinclair David R. , Maharani Asri , Stow Daniel , Welsh Claire E. , Matthews Fiona E.
Published: November 2021

Background

COVID-19 vaccination in many countries, including England, has been prioritised primarily by age. However, people of the same age can have very different health statuses. Frailty is a commonly used metric of health and has been found to be more strongly associated with mortality than age among COVID-19 inpatients.

Methods

We compared the number of first vaccine doses administered across the 135 NHS Clinical Commissioning Groups (CCGs) of England to both the over 50 population and the estimated frail population in each area. Area-based frailty estimates were generated using the English Longitudinal Survey of Ageing (ELSA), a national survey of older people. We also compared the number of doses to the number of people with other risk factors associated with COVID-19: atrial fibrillation, chronic kidney disease, diabetes, learning disabilities, obesity and smoking status.

Results

We estimate that after 79 days of the vaccine program, across all Clinical Commissioning Group areas, the number of people who received a first vaccine per frail person ranged from 4.4 (95% CI 4.0-4.8) and 20.1 (95% CI 18.3-21.9). The prevalences of other risk factors were also poorly associated with the prevalence of vaccination across England.

Conclusions

Vaccination with age-based priority created area-based inequities in the number of doses administered relative to the number of people who are frail or have other risk factors associated with COVID-19. As frailty has previously been found to be more strongly associated with mortality than age for COVID-19 inpatients, an age-based priority system may increase the risk of mortality in some areas during the vaccine roll-out period. Authorities planning COVID-19 vaccination programmes should consider the disadvantages of an age-based priority system.
Assessment of in vitro activities of novel modified antimicrobial peptides against clarithromycin resistant Mycobacterium abscessus
Authors: Sudadech Phantitra , Roytrakul Sittiruk , Kaewprasert Orawee , Sirichoat Auttawit , Chetchotisakd Ploenchan , Kanthawong Sakawrat , Faksri Kiatichai
Published: November 2021

Mycobacterium abscessus (Mab) is one of the most drug resistant bacteria with a high treatment failure rate. Antimicrobial peptides (AMPs) are alternative therapeutic agents against this infection. This study was aimed to assess the in vitro activities of thirteen AMPs (S5, S52, S6, S61, S62, S63, KLK, KLK1, KLK2, Pug-1, Pug-2, Pug-3 and Pug-4) that have never been investigated against drug resistant Mab isolates. Only four novel modified AMPs (S61, S62, S63 and KLK1) provided the lowest minimum inhibitory concentration (MIC) values ranging from 200–400 μg/ml against the Mab ATCC19977 strain. These four potential AMPs were further tested with 16 clinical isolates of clarithromycin resistant Mab. The majority of the tested strains (10/16 isolates, 62.5%) showed ~99% kill by all four AMPs within 24 hours with an MIC <50 μg/ml. Only two isolates (12.5%) with acquired clarithromycin resistance, however, exhibited values <50 μg/ml of four potential AMPs, S61, S62, S63 and KLK1 after 3-days-incubation. At the MICs level, S63 showed the lowest toxicity with 1.50% hemolysis and 100% PBMC viability whereas KLK1 showed the highest hemolysis (10.21%) and lowest PBMC viability (93.52%). S61, S62 and S63 were further tested with clarithromycin-AMP interaction assays and found that 5/10 (50%) of selected isolates exhibited a synergistic interaction with 0.02–0.41 FICI values. This present study demonstrated the potential application of novel AMPs as an adjunctive treatment with clarithromycin against drug resistant Mab infection.
Prevalence of HBsAg among reproductive age couples in Chongqing: A population-based, cross-sectional study
Authors: Chen Qing , Liu Jun , He Yang , Yang Liu , Luo Huiqiang , Wang Yixi , Zhang Xuewen , Li Ningxiu
Published: November 2021

Hepatitis B is a leading cause of death worldwide. Here, we performed a large, population-based, cross-sectional study in Chongqing, China from 2011 to 2016 to assess the prevalence of HBsAg among couples of reproductive age, to predict subsequent trends, and to provide evidence for the WHO goal of "the elimination of viral hepatitis as a public health threat by 2030". A total of 386,286 couples aged 20 to 49 years were enrolled in the study. Approximately 14.35% of couples were HBsAg positive, including 95.00% with discordant HBsAg positivity. HBsAg prevalence was higher in men than in women. Among different occupations, the two categories of “houseworker” (female 6.73%, male 9.99%) and “unemployed” (female 6.64%, male 9.94%) showed the highest HBsAg positivity. In different regions, the lowest prevalence appeared in southeastern Chongqing (female 4.87%, male 7.71%). In 2030, the HBsAg positivity rate is expected to be 2.79%, 7.27% and 5.13% in females, males, and the whole population, respectively. According to the trends, this rate would drop to less than 2% in 2034, 2078 and 2051. In conclusion, the HBsAg prevalence in Chongqing is still relatively high compared with that in other parts of western China, especially among reproductive-age men. HBsAg-positive couples should be taken as an important unit of care. Vaccination is necessary before pregnancy if no antibody is found. More attention should be given to people without stable jobs. HBsAg-positive rate will decrease perceptibly by 2030 and will reach the level of low in epidemic areas by 2050.
COVID-19 vaccination perceptions and intentions of maternity care consumers and providers in Australia
Authors: Bradfield Zoe , Wynter Karen , Hauck Yvonne , Sweet Linda , Wilson Alyce N. , Szabo Rebecca A. , Vasilevski Vidanka , Kuliukas Lesley , Homer Caroline S. E.
Published: November 2021

Introduction

Vaccination against COVID-19 is a key global public health strategy. Health professionals including midwives and doctors support and influence vaccination uptake by childbearing women. There is currently no evidence regarding the COVID-19 vaccination perceptions and intentions of those who receive or provide maternity care in Australia. The aim of this study was to address this gap in knowledge and explore the perceptions and intentions regarding COVID-19 vaccination from consumers and providers of maternity care in Australia.

Methods

A national cross-sectional online study conducted in early 2021 in Australia, a country that has had a very low number of COVID-19 cases and deaths. Recruitment was undertaken through parenting and health professional social media sites and professional college distribution lists. A total of 853 completed responses, from women (n = 326), maternity care providers including doctors (n = 58), midwives (n = 391) and midwifery students (n = 78).

Findings

Personal intention to be vaccinated ranged from 48–89% with doctors most likely and women least likely. Doctors and midwifery students were significantly more likely to recommend the vaccine to pregnant women in their care than midwives (p<0.001). Fewer doctors (2%) felt that women should wait until breastfeeding had concluded before being vaccinated compared with 24% of midwives and 21% of midwifery students (p<0.001). More than half of the midwives (53%) had concerns about the COVID-19 vaccine for the women in their care compared with 35% of doctors and 46% of midwifery students. Despite national guidelines recommending vaccination of breastfeeding women, 54% of practitioners were unlikely to recommend vaccination for this group.

Conclusion

This is the first study to explore the perceptions and intentions regarding COVID-19 vaccination from the perspective of those who receive and provide maternity care in Australia. Findings have utility to support targeted public health messaging for these and other cohorts.
Applied aerial spectroscopy: A case study on remote sensing of an ancient and semi-natural woodland
Authors: Ahmed Shara , Nicholson Catherine E. , Muto Paul , Perry Justin J. , Dean John R.
Published: November 2021

An area of ancient and semi-natural woodland (ASNW) has been investigated by applied aerial spectroscopy using an unmanned aerial vehicle (UAV) with multispectral image (MSI) camera. A novel normalised difference spectral index (NDSI) algorithm was developed using principal component analysis (PCA). This novel NDSI was then combined with a simple segmentation method of thresholding and applied for the identification of native tree species as well as the overall health of the woodland. Using this new approach allowed the identification of trees at canopy level, across 7.4 hectares (73,934 m2) of ASNW, as oak (53%), silver birch (37%), empty space (9%) and dead trees (1%). This UAV derived data was corroborated, for its accuracy, by a statistically valid ground-level field study that identified oak (47%), silver birch (46%) and dead trees (7.4%). This simple innovative approach, using a low-cost multirotor UAV with MSI camera, is both rapid to deploy, was flown around 100 m above ground level, provides useable high resolution (5.3 cm / pixel) data within 22 mins that can be interrogated using readily available PC-based software to identify tree species. In addition, it provides an overall oversight of woodland health and has the potential to inform a future woodland regeneration strategy.
MK2a inhibitor CMPD1 abrogates chikungunya virus infection by modulating actin remodeling pathway
Authors: Mamidi Prabhudutta , Nayak Tapas Kumar , Kumar Abhishek , Kumar Sameer , Chatterjee Sanchari , De Saikat , Datey Ankita , Ghosh Soumyajit , Keshry Supriya Suman , Singh Sharad , Laha Eshna , Ray Amrita , Chattopadhyay Subhasis , Chattopadhyay Soma
Published: November 2021

Chikungunya virus (CHIKV) epidemics around the world have created public health concern with the unavailability of effective drugs and vaccines. This emphasizes the need for molecular understanding of host-virus interactions for developing effective targeted antivirals. Microarray analysis was carried out using CHIKV strain (Prototype and Indian) infected Vero cells and two host isozymes, MAPK activated protein kinase 2 (MK2) and MAPK activated protein kinase 3 (MK3) were selected for further analysis. The substrate spectrum of both enzymes is indistinguishable and covers proteins involved in cytokines production, endocytosis, reorganization of the cytoskeleton, cell migration, cell cycle control, chromatin remodeling and transcriptional regulation. Gene silencing and drug treatment were performed in vitro and in vivo to unravel the role of MK2/MK3 in CHIKV infection. Gene silencing of MK2 and MK3 abrogated around 58% CHIKV progeny release from the host cell and a MK2 activation inhibitor (CMPD1) treatment demonstrated 68% inhibition of viral infection suggesting a major role of MAPKAPKs during late CHIKV infection in vitro. Further, it was observed that the inhibition in viral infection is primarily due to the abrogation of lamellipodium formation through modulation of factors involved in the actin cytoskeleton remodeling pathway. Moreover, CHIKV-infected C57BL/6 mice demonstrated reduction in the viral copy number, lessened disease score and better survivability after CMPD1 treatment. In addition, reduction in expression of key pro-inflammatory mediators such as CXCL13, RAGE, FGF, MMP9 and increase in HGF (a CHIKV infection recovery marker) was observed indicating the effectiveness of the drug against CHIKV. Taken together it can be proposed that MK2 and MK3 are crucial host factors for CHIKV infection and can be considered as important target for developing effective anti-CHIKV strategies.
Raman spectroscopic analysis of skin as a diagnostic tool for Human African Trypanosomiasis
Authors: Girard Alexandre , Cooper Anneli , Mabbott Samuel , Bradley Barbara , Asiala Steven , Jamieson Lauren , Clucas Caroline , Capewell Paul , Marchesi Francesco , Gibbins Matthew P. , Hentzschel Franziska , Marti Matthias , Quintana Juan F. , Garside Paul , Faulds Karen , MacLeod Annette , Graham Duncan
Published: November 2021

Human African Trypanosomiasis (HAT) has been responsible for several deadly epidemics throughout the 20th century, but a renewed commitment to disease control has significantly reduced new cases and motivated a target for the elimination of Trypanosoma brucei gambiense-HAT by 2030. However, the recent identification of latent human infections, and the detection of trypanosomes in extravascular tissues hidden from current diagnostic tools, such as the skin, has added new complexity to identifying infected individuals. New and improved diagnostic tests to detect Trypanosoma brucei infection by interrogating the skin are therefore needed. Recent advances have improved the cost, sensitivity and portability of Raman spectroscopy technology for non-invasive medical diagnostics, making it an attractive tool for gambiense-HAT detection. The aim of this work was to assess and develop a new non-invasive diagnostic method for T. brucei through Raman spectroscopy of the skin. Infections were performed in an established murine disease model using the animal-infective Trypanosoma brucei brucei subspecies. The skin of infected and matched control mice was scrutinized ex vivo using a confocal Raman microscope with 532 nm excitation and in situ at 785 nm excitation with a portable field-compatible instrument. Spectral evaluation and Principal Component Analysis confirmed discrimination of T. brucei-infected from uninfected tissue, and a characterisation of biochemical changes in lipids and proteins in parasite-infected skin indicated by prominent Raman peak intensities was performed. This study is the first to demonstrate the application of Raman spectroscopy for the detection of T. brucei by targeting the skin of the host. The technique has significant potential to discriminate between infected and non-infected tissue and could represent a unique, non-invasive diagnostic tool in the goal for elimination of gambiense-HAT as well as for Animal African Trypanosomiasis (AAT).
Infection, recovery and re-infection of farmed mink with SARS-CoV-2
Authors: Rasmussen Thomas Bruun , Fonager Jannik , Jørgensen Charlotte Sværke , Lassaunière Ria , Hammer Anne Sofie , Quaade Michelle Lauge , Boklund Anette , Lohse Louise , Strandbygaard Bertel , Rasmussen Morten , Michaelsen Thomas Yssing , Mortensen Sten , Fomsgaard Anders , Belsham Graham J. , Bøtner Anette
Published: November 2021

Mink, on a farm with about 15,000 animals, became infected with SARS-CoV-2. Over 75% of tested animals were positive for SARS-CoV-2 RNA in throat swabs and 100% of tested animals were seropositive. The virus responsible had a deletion of nucleotides encoding residues H69 and V70 within the spike protein gene as well as the A22920T mutation, resulting in the Y453F substitution within this protein, seen previously in mink. The infected mink recovered and after free-testing of 300 mink (a level giving 93% confidence of detecting a 1% prevalence), the animals remained seropositive. During further follow-up studies, after a period of more than 2 months without any virus detection, over 75% of tested animals again scored positive for SARS-CoV-2 RNA. Whole genome sequencing showed that the viruses circulating during this re-infection were most closely related to those identified in the first outbreak on this farm but additional sequence changes had occurred. Animals had much higher levels of anti-SARS-CoV-2 antibodies in serum samples after the second round of infection than at free-testing or during recovery from initial infection, consistent with a boosted immune response. Thus, it was concluded that following recovery from an initial infection, seropositive mink were readily re-infected by SARS-CoV-2.
The construction of a growth-oriented global climate agenda: a critical historical analysis
Authors: Torres Cati , Moranta Joan , Murray Ivan
Published: November 2021

By the end of 2019, more than 11,000 world scientists declared Planet Earth is facing a climate emergency, which signals the failure of the global climate agenda (GCA). Since it took off thirty years ago, emissions have continued to increase at the planetary level. We add to the literature focusing on the economic and political dimensions shaping the GCA. In particular, we examine its economic growth roots under the umbrella of sustainable development (SD) or green growth to shed some light on whether the rules driving the world economy are shaping it. Such rules are built on the growth ideology fuelling the current extractivist socioeconomic metabolism, which in turn lies behind the socioecological crisis. We review the main international climate-focused events and document a shift in the guiding principles of climate politics from the 1980s onwards under which growth is no longer viewed as a driver of climate change (CC) but as its solution. We argue that the strategy to promote growth-based SD represents the main cause of policy failure. Indeed, the result is a policy that is highly reliant on technological solutions and market-based instruments and leads to the belief that green growth is both possible and the solution to CC. Such a belief restricts the debate to the economy’s ‘decarbonisation’ and CC adaptation and overlooks other important socio-political aspects involve in climate action.
MODERATE, REGULAR ALCOHOL CONSUMPTION IS ASSOCIATED WITH HIGHER COGNITIVE FUNCTION IN OLDER COMMUNITY-DWELLING ADULTS
Authors: Reas E.T. , Laughlin G.A. , Kritz-Silverstein D. , Barrett-Connor E. , McEvoy L.K.
Published: November 2021

Background: Evidence suggests that moderate alcohol consumption may protect against cognitive decline and dementia. However, uncertainty remains over the patterns of drinking that are most beneficial. Objective: To examine associations between amount and frequency of alcohol consumption with multiple domains of cognitive function in a well-characterized cohort of older community-dwelling adults in southern California. Design: Observational, cross-sectional cohort study. Setting: A research visit between 1988-1992 in Rancho Bernardo, California. Participants: 1624 participants of the Rancho Bernardo Study (mean age ± SD = 73.2 ± 9.3 years). Measurements: Participants completed a neuropsychological test battery, self-administered questionnaires on alcohol consumption and lifestyle, and a clinical health evaluation. We classified participants according to average amount of alcohol intake into never, former, moderate, heavy and excessive drinkers, and according to frequency of alcohol intake, into non-drinkers, rare, infrequent, frequent and daily drinkers. We examined the association between alcohol intake and cognitive function, controlling for age, sex, education, exercise, smoking, waist-hip ratio, hypertension and self-assessed health. Results: Amount and frequency of alcohol intake were significantly associated with cognitive function, even after controlling for potentially related health and lifestyle variables. Global and executive function showed positive linear associations with amount and frequency of alcohol intake, whereas visual memory showed an inverted U-shaped association with alcohol intake, with better performance for moderate and infrequent drinkers than for non-drinkers, excessive drinkers or daily drinkers. Conclusions: In several cognitive domains, moderate, regular alcohol intake was associated with better cognitive function relative to not drinking or drinking less frequently. This suggests that beneficial cognitive effects of alcohol intake may be achieved with low levels of drinking that are unlikely to be associated with adverse effects in an aging population.
THE EFFECT OF VASCULAR NEUROPATHOLOGY ON LATE-LIFE COGNITION: RESULTS FROM THE SMART PROJECT
Authors: Kryscio R.J. , Abner E.L. , Nelson P.T. , Bennett D. , Schneider J. , Yu L. , Hemmy L.S. , Lim K.O. , Masaki K. , Cairns N. , Xiong C. , Woltjer R. , Dodge H.H. , Tyas S. , Fardo D.W. , Lou W. , Wan L. , Schmitt F.A.
Published: November 2021

Background: Cerebral vascular pathology may contribute to cognitive decline experienced by some elderly near death. Given evidence for mixed neuropathologies in advanced age, preventing or reducing cerebrovascular burden in late life may be beneficial. Objective: To correlate measures of cerebral vascular pathology with cognitive trajectories. Setting: Observational study. Participants: A cohort of 2,274 individuals who came to autopsy at a mean age of 89.3 years and 82 percent of whom had at least two cognitive assessments within the last six years of life was compiled from six centers conducting longitudinal studies. Measurements: For each cognitive domain: immediate and delayed memory, language, and naming, three trajectories were examined: good, intermediate, and poor cognition. The probability of a participant belonging to each trajectory was associated with measures of cerebral vascular pathology after adjustment for demographics, APOE, and Alzheimer neuropathology. Results: A large proportion of the cohort (72-94%) experienced good or intermediate cognition in the four domains examined. The presence of arteriolosclerosis and the presence of lacunar infarcts doubled the odds of belonging to the poor cognitive trajectory for language when compared to the good trajectory. The presence of lacunar infarcts increased the odds of an intermediate or poor trajectory for immediate and delayed recall while the presence of large artery infarcts increased the odds of poor trajectories for all four cognitive domains examined. Microinfarcts and cerebral amyloid angiopathy had little effect on the trajectories. Conclusion: Indicators of cerebral vascular pathology act differently on late life cognition.
Kinetics and Equilibrium Modeling of Single and Binary Adsorption of Aluminum(III) and Copper(II) Onto Calamansi (Citrofortunella microcarpa) Fruit Peels
Authors: Binauhan Melanie G. , Adornado Adonis P. , Tayo Lemmuel L. , Soriano Allan N. , C. Rubi Rugi Vicente
Published: November 2021

The introduction of heavy metal wastes in the environment has posed health risks to both human and animals due to their toxicity. Since then, different studies have been explored for the possibility of utilizing new, low–cost, and sustainable adsorbent materials to get rid of heavy metals in the wastewater streams and aqueous solutions. This present study aimed to investigate and compare the adsorption ability of powdered calamansi (Citrofortunella microcarpa) fruit peels (PCFP) for the elimination of both Al(III) and Cu(II) ions in single (non–competitive) and binary (competitive) aqueous systems by batch adsorption techniques. Scanning electron microscopic and spectroscopic techniques were used to characterize the surface morphologies for the biosorbent and quantify the removal rates of heavy metal, respectively. Models were then used to describe in detail about the adsorption kinetics and isotherms for both single and binary metal systems. The influence and dependency of different experimental conditions on adsorption performance were also analyzed. The PCFP derived biosorbent was successful in removal of both Al(III) and Cu(II) ions in single (non–competitive) and binary (competitive) aqueous systems with 99, 70 and 91% adsorption rates, respectively. The biosorption process follows the Ho’s pseudo–second order kinetics. Furthermore, the Langmuir isotherm model was found helpful in explaining the adsorption mechanism. The dominating electrostatic interaction between adsorbents and adsorbates demonstrates monolayer adsorption at the binding sites on the surface of the peeling. Finally, the findings of this study will contribute to a better understanding of the adsorption process, as well as future system design applications in the treatment of heavy metal containing waste effluents.
Pattern of Alpha Fetoprotein and Activities of Liver Enzymes in Type 2 Diabetic Patients Attending Nnamdi Azikiwe University Teaching Hospital Nnewi, Anambra State, Nigeria.
Authors: Ogbodo Emmanuel Chukwuemeka , Ezeugwunne Ifeoma Priscilla , Idris Stephen Shehu , Nnaemeka Wurola Serah , DavidIbimere Obinna , Elosuba Nwanneka Victoria , Myke-Mbata Blessing K. , Edward Ukamaka , Nnamdi Johnjude Chinonso , Nwanguma Eberechi D.
Published: November 2021

Diabetes mellitus (DM) is a metabolic disorder characterized by hyperglycemia, which has become health problem of global interest, linked to renal disease. The study is to assess the activities of some liver enzymes and alpha fetoprotein level in type 2 diabetic patients. 81 participants comprised 38 diabetics and 43 controls were randomly recruited. Informed, oral and written consent was obtained from the participants. Ethical approval was obtained. 6mls of fasting blood samples were collected. Plasma glucose, alpha feto-protein (AFP) levels; serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and gamma glutamyltransferase (GGT) activities were analyzed using standard laboratory methods.  The results showed significantly increased plasma glucose level in the diabetics than in controls (p=0.000), as well as in male diabetics than in female diabetics (p=0.048). But, the mean age, AFP, ALT, AST, ALP and GGT activities  were the same in the diabetics and the controls; and in male and female diabetics  at p>0.05 in each case. Relatively strong positive significant correlation was observed between ALT Vs AST (r=0.566; p=0.000) and a weaker negative association exist between ALT Vs Age (r= -0.353; p=0.022) in diabetics but no associations were observed with other parameters studied. In conclusion the positive association between ALT and AST may reveal transaminase derangements in liver status of type 2 diabetic subjects studied.
Handwritten Signature Biometric Data Analysis for Personality Prediction System Using Machine Learning Techniques
Authors: Gornale Shivanand S. , Kumar Sathish , Hiremath Prakash S.
Published: November 2021

Handwritten signature has been considered as one of the most widely accepted behavioral personal trait in Biometric security system; and  It contains various dynamic and innate behavioral traits like shapes and patterns which can certainly find a person’s soft characteristics like age, gender, Personality, handedness and many more. Person’s signature or handwriting determines the state of the person’s mind or personality characteristics at the time of writing. This paper provides a personality prediction system of different characteristics determining the personality of a person based on offline handwritten signature Images. Experiments are carried out using supervised learning techniques. Results shows a significant recognition rate and validates the effectiveness against the state-of-art techniques in comparison to similar works.
Decision analysis support for evaluating transmission risk of COVID-19 in places where people gather
Authors: Hongoh Valerie , Maybury David , Levesque Jérôme , Fazil Aamir , Otten Ainsley , Turgeon Patricia , Waddell Lisa , Ogden Nicholas H
Published: November 2021

Background: The coronavirus diseases 2019 (COVID-19) pandemic has presented an unprecedented public health challenge. Prior to vaccination, non-pharmaceutical interventions, including closures, were necessary to help control the epidemic. With the arrival of variants of concern and insufficient population vaccination coverage, ongoing evaluation of transmission risk in settings and the use of non-pharmaceutical interventions are necessary to help control the epidemic. This study aimed to produce a framework for evaluating transmission risk in settings where individuals gather and inform decision-making. Methods: A multi-criteria decision analysis process was used to structure the framework. Fifteen criteria were identified as important to consider for COVID-19 transmission risk based on the literature. This list was ranked by experts and then categorized. The analysis was structured by the consensus list of criteria and relative positioning of each criteria within the list to produce sets of factors to consider when assessing transmission risk at gatherings. Results: Fifteen experts from across Canada participated in ranking the criteria. Strong consensus was found on the relative importance of criteria and this relative consensus was used to create four categories: critical (3 criteria); important (6 criteria); good to consider (5 criteria); and if time permits (1 criterion). Conclusion: The resulting consensus list and categories constitutes a set of important elements that can be applied to any setting as an objective and transparent framework to assess transmission risk in the venue. In conjunction with further consideration of the local epidemiology of COVID-19, an overall risk of transmission assessment can be established and uniformly implemented.
Multisystem inflammatory syndrome in children in Canada
Authors: Laverty Meghan , Salvadori Marina , Squires Susan G , Ahmed May , Eisenbeis Lisa , Lee Santina , Des Cormiers Annick , Li Y Anita
Published: November 2021

This article provides a summary of the epidemiology of multisystem inflammatory syndrome in children (MIS-C) cases reported nationally in Canada by provincial and territorial health authorities. Multisystem inflammatory syndrome in children is a post-viral inflammatory syndrome that temporally follows coronavirus disease 2019 (COVID-19). Symptoms may include fever, abdominal pain, vomiting, diarrhea, skin rash and other signs of inflammation. In Canada, MIS-C is rare, with 269 cases reported to the Public Health Agency of Canada between March 11, 2020 and October 2, 2021. One hundred forty-two (53%) of these cases were lab-confirmed COVID-19 cases or epidemiologically-linked with COVID-19 cases. Cases have been reported in infants as young as one week to youth as old as 18 years, with a median age of six years. Cases were more likely to occur in males than females (58% vs 42%, respectively; p=0.006). Almost all MIS-C cases (99%) required hospitalization and 36% required intensive care unit admission. No deaths have been reported to date. The time trend of MIS-C aligns with the incidence rate time trend of COVID-19 reported in children, with a two to six-week lag.