bluuebunny/crossref_metadata_2025 huggingface dataset:
Perspective Swap from Central Europe to East Asia: How Relevant Is Urban Environmental Acupuncture in Small-Scale Green Space Development in the Context of the Republic of Korea?
Authors: Song Jiyoon , Hemingway Jessica , Park Chang Sug
Published: February 2024

A lack of green space, driven by intense urbanization, has resulted in adverse effects on human life and ecosystems. These adverse effects include, but are not limited to, urban heat islands, disruption to ecological networks, and fragmentation of human and animal habitats. Despite the critical need to improve climate resilience through green infrastructure expansion, not enough is being done to improve conditions globally. This study investigates the Urban Environmental Acupuncture (UEA) concept, exploring its potential application in Korea to implement green infrastructure in dense urban areas. Korea was selected as a case study due to its high population density and the urgent long-term need to safeguard urban green spaces. Semi-structured interviews with experts working in park and green space policy among Korean local governments were conducted. The interviews were analyzed using content analysis based on research questions. The results point to challenges in applying the UEA concept related to Korea’s urban green space policies, including land acquisition difficulties, insufficient information and research, and difficulties in continuous management with micro green spaces. Moreover, we provide strategies to overcome the challenges of UEA implementation within Korea. The findings and proposed strategies offer insight to those facing similar conditions such as high population density and limited delegated land for green space expansion.
Field-Scale Winter Wheat Growth Prediction Applying Machine Learning Methods with Unmanned Aerial Vehicle Imagery and Soil Properties
Authors: Nduku Lwandile , Munghemezulu Cilence , Mashaba-Munghemezulu Zinhle , Masiza Wonga , Ratshiedana Phathutshedzo Eugene , Kalumba Ahmed Mukalazi , Chirima Johannes George
Published: February 2024

Monitoring crop growth conditions during the growing season provides information on available soil nutrients and crop health status, which are important for agricultural management practices. Crop growth frequently varies due to site-specific climate and farm management practices. These variations might arise from sub-field-scale heterogeneities in soil composition, moisture levels, sunlight, and diseases. Therefore, soil properties and crop biophysical data are useful to predict field-scale crop development. This study investigates soil data and spectral indices derived from multispectral Unmanned Aerial Vehicle (UAV) imagery to predict crop height at two winter wheat farms. The datasets were investigated using Gaussian Process Regression (GPR), Ensemble Regression (ER), Decision tree (DT), and Support Vector Machine (SVM) machine learning regression algorithms. The findings showed that GPR (R2 = 0.69 to 0.74, RMSE = 15.95 to 17.91 cm) has superior accuracy in all models when using vegetation indices (VIs) to predict crop growth for both wheat farms. Furthermore, the variable importance generated using the GRP model showed that the RedEdge Normalized Difference Vegetation Index (RENDVI) had the most influence in predicting wheat crop height compared to the other predictor variables. The clay, calcium (Ca), magnesium (Mg), and potassium (K) soil properties have a moderate positive correlation with crop height. The findings from this study showed that the integration of vegetation indices and soil properties predicts crop height accurately. However, using the vegetation indices independently was more accurate at predicting crop height. The outcomes from this study are beneficial for improving agronomic management within the season based on crop height trends. Hence, farmers can focus on using cost-effective VIs for monitoring particular areas experiencing crop stress.
Study of the Effect of Static Eccentricity on Vibration Damping Properties of Squeeze Film Dampers Considering the Two-Phase Flow Case
Authors: Zhou Hailun , Fang Liang , Zhang Ming , Cao Gangyi , Su Jianyang
Published: February 2024

To analyze the effect of static eccentricity on the air ingestion distribution and vibration damping properties of the SFD, a numerical simulation study of SFDs considering two-phase flow was carried out based on CFD using a transient solution method and dynamic mesh technique. The results show that the angle between the static eccentricity direction and the circumferential direction of the oil supply hole increases and the air ingestion area in the oil film expands. In contrast, the oil film damping decreases, and the larger the static eccentricity distance, the greater its effect on the air ingestion area in the oil film. When the circumferential angle is small, the oil film damping increases with the increase of static eccentricity distance, and when the circumferential angle is large, the oil film damping decreases with the increase of static eccentricity distance and then increases. With the increase of static eccentricity distance, the air ingestion area at both ends of the oil film increases. At the same time, studying the effect of dynamic eccentricity shows that as the dynamic eccentricity increases, the oil film damping first decreases and then increases, and the air ingestion area increases. Comparing the 1 hole, the 2 hole, and the 3 hole oil supplies, the air ingestion area is significantly larger in the 1 hole oil supply than in the 2 hole or the 3 hole oil supplies, and the oil film damping of the 1 hole oil supply is smaller than the oil film damping of the 2 hole or the 3 hole oil supplies. It can be seen from the present study that in the actual installation of the SFD, when the circumferential angle is less than 60°, the static eccentricity can be increased appropriately. When the circumferential angle is greater than 60°, the static eccentricity can be appropriately reduced.
Influence of Various Heat Treatments on Microstructures and Mechanical Properties of GH4099 Superalloy Produced by Laser Powder Bed Fusion
Authors: Liu Jiahao , Wang Yonghui , Guo Wenqian , Wang Linshan , Zhang Shaoming , Hu Qiang
Published: February 2024

The microstructures and mechanical properties of a γ′-strengthened nickel-based superalloy, GH4099, produced by laser powder bed fusion, at room temperature and 900 °C are investigated, followed by three various heat treatments. The as-built (AB) alloy consists of cellular/dendrite substructures within columnar grains aligning in <100> crystal orientation. No γ′ phase is observed in the AB sample due to the relatively low content of Al +Ti. Following the standard solid solution treatment, the molten pool boundaries and cellular/dendrite substructures disappear, whilst the columnar grains remain. The transformation of columnar grains to equiaxed grains occurs through the primary solid solution treatment due to the recovery and recrystallization process. After aging at 850 °C for 480 min, the carbides in the three samples distributed at grain boundaries and within grains and the spherical γ′ phase whose size is about 43 nm ± 16 nm develop in the standard solid solution + aging and primary solid solution + aging samples (SA and PA samples) while the bimodal size of cubic (181 nm ± 85 nm) and spherical (43 nm ± 16 nm) γ′ precipitates is presented in the primary solid solution + secondary solid solution + aging sample (PSA samples). The uniaxial tensile tests are carried out at room temperature (RT) and 900 °C. The AB sample has the best RT ductility (~51% of elongation and ~67% of area reduction). Following the three heat treatments, the samples all acquire excellent RT tensile properties (>750 MPa of yield strengths and >32% of elongations). However, clear ductility dips and intergranular fracture modes occur during the 900 °C tensile tests, which could be related to carbide distribution and a change in the deformation mechanism.
Recent Progress in Modification of Polyphenylene Oxide for Application in High-Frequency Communication
Authors: Liao Lingyuan , Ruan Wenhong , Zhang Mingqiu , Lin Musong
Published: February 2024

With the rapid development of highly integrated electronic devices and high-frequency microwave communication technology, the parasitic resistance–capacitance (RC) delay and propagation loss severely restrict the development of a high-frequency communication system. Benefiting from its low dielectric constants (Dk) and low dielectric loss factor (Df), polyphenylene oxide (PPO) has attracted widespread attention for its application in the dielectric layers of integrated circuits. However, PPO suffers from a very high melting viscosity, a larger coefficient of thermal expansion than copper wire and poor solvent resistance. Recently, many efforts have focused on the modification of PPO by various means for communication applications. However, review articles focusing on PPO are unexpectedly limited. In this article, the research progress concerning PPO materials in view of the modification of PPO has been summarized. The following aspects are covered: polymerization and design of special chemical structure, low molecular weight PPO and blending with thermosetting resin, hyperbranched PPO, thermosetting PPO and incorporating with fillers. In addition, the advantages and disadvantages of various types of modification methods and their applications are compared, and the possible future development directions are also proposed. It is believed that this review will arouse the interest of the electronics industry because of the detailed summary of the cutting-edge modification technology for PPO.
An Analysis of the Kinematical Characteristics of an Eccentric Curve-Face Gear Pair with Compound Motion
Authors: He Chunjiang , Zhang Jinxu , Lin Chao
Published: February 2024

An atypical face gear pair with complex transmission motion can be used in intermittent reciprocating mechanisms with more precise transmission and a much higher capacity than conventional mechanisms, such as cams and linkages. In this study, we derive a mathematical equation for the complex tooth surface of this gear pair. We indicate the change in root cutting, top sharpening and the effective width of the tooth surface with different parameters. Additionally, we derive the governing equation for the kinematical characteristics of this eccentric curve-face gear pair with a rigid–flexible coupling system, revealing the continuous intermittent contact principle of this gear type with different parameters. Boundary conditions for the gear pair are proposed, demonstrating that the vibration of the gear pair is more obvious, even at a low velocity. In addition, the critical velocity, which mostly ranges from 300 rpm to 400 rpm, is affected by the stiffness of the frames and the parameters of the tooth surfaces. The interval space and interval time of the intermittent contact system are Δd≤0.3 mm and Δt≤5.6×10−4 s, with visible surface sliding on the contact area. It is shown that the contact points are firstly concentrated at the outer part of the tooth surface and that the meshing will break off at the first tooth with the minimum inner radius RGi−min. These theoretical results, which have been verified experimentally, provide theoretical support for further analysis and the better application of this unconventional gear pair.
Comparative Analysis of Psychophysiological Responses in Fibromyalgia Patients: Evaluating Neuromodulation Alone, Neuromodulation Combined with Virtual Reality, and Exercise Interventions
Authors: Rubio-Zarapuz Alejandro , Apolo-Arenas María Dolores , Tomas-Carus Pablo , Tornero-Aguilera José Francisco , Clemente-Suárez Vicente Javier , Parraca Jose A.
Published: February 2024

Background and Objectives: Fibromyalgia, a chronic condition, manifests as widespread musculoskeletal pain, fatigue, sleep disturbances, autonomic and cognitive dysfunction, hypersensitivity to stimuli, and various somatic and psychiatric symptoms. This study, a controlled and randomized experiment, aimed to evaluate and compare the immediate effects of different treatments on fibromyalgia patients. Materials and Methods: The treatments included the EXOPULSE Mollii suit, a combination of the EXOPULSE Mollii suit with a virtual reality (VR) protocol, and a physical exercise regimen. A cohort of 89 female fibromyalgia patients was randomly assigned to one of four groups: Control (n = 20), Suit only (n = 22), Suit combined with VR (n = 21), and Exercise (n = 26). Results: This study found notable differences across the groups in several key parameters. In the Control group, significant changes were observed in Forced Expiratory Volume (FEV 1/FEV 6), the Numeric Rating Scale (NRS) for pain, Pressure Pain Threshold (PPT) at the epicondyle, cortical arousal levels, the 10 m up-and-go test, and in all measured variables related to temperature and muscle oxygenation. For the group using the suit alone, there were significant differences noted in the NRS, the chair stand test, palm temperature, and all muscle oxygenation parameters. The Suit + VR group showed significant changes in the NRS, PPT at the knee, handgrip strength test, the 10 m up-and-go test, one-leg balance test with the right leg, muscle oxygen saturation (SmO2), deoxygenated hemoglobin (HHb), and oxygenated hemoglobin (O2Hb). Finally, the Exercise group exhibited significant differences in FEV 1/FEV 6, chest perimeter difference, NRS, PPT at both the epicondyle and knee, cortical arousal, the chair stand test, the 10-m up-and-go test, and in SmO2, HHb, and O2Hb levels. Conclusions: combining neuromodulation with VR and targeted exercise regimens can effectively alleviate fibromyalgia symptoms, offering promising avenues for non-pharmacological management.
Emphysematous Pancreatitis as a Life-Threatening Condition: A Case Report and Review of the Literature
Authors: Filipović Aleksandar , Mašulović Dragan , Bulatović Dušan , Zakošek Miloš , Igić Aleksa , Filipović Tamara
Published: February 2024

Emphysematous pancreatitis represents the presence of gas within or around the pancreas on the ground of necrotizing pancreatitis due to superinfection with gas-forming bacteria. This entity is diagnosed on clinical grounds and on the basis of radiologic findings. Computed tomography is the preferred imaging modality used to detect this life-threating condition. The management of emphysematous pancreatitis consists of conservative measures, image-guided percutaneous catheter drainage or endoscopic therapy, and surgical intervention, which is delayed as long as possible and undertaken only in patients who continue to deteriorate despite conservative management. Due to its high mortality rate, early and prompt recognition and treatment of emphysematous pancreatitis are crucial and require individualized treatment with the involvement of a multidisciplinary team. Here, we present a case of emphysematous pancreatitis as an unusual occurrence and discuss disease features and treatment options in order to facilitate diagnostics and therapy.
Phase Equilibrium Studies of Nonferrous Smelting Slags: A Review
Authors: Xie Sui , Zhao Baojun
Published: February 2024

Pyrometallurgy is the primary technique for the production of many nonferrous metals such as copper, lead, and zinc. The phase equilibrium information of smelting slags plays an important role in the efficient extraction of metals and energy consumption. The experimental technologies used in phase equilibrium studies are compared. The presentation and applications of the pseudo-ternary and pseudo-binary phase diagrams are demonstrated in the Fe–Si–Ca–Zn–Mg–Al–Cu–S–O system. Experimental results are also compared with the predictions of FactSage to evaluate the accuracy of the current thermodynamic database. This review paper provides comprehensive information for the operation of nonferrous metals and optimization of the thermodynamic database.
5-ALA, DTA-6, and Nitrogen Mitigate NaCl Stress by Promoting Photosynthesis and Carbon Metabolism in Rice Seedlings
Authors: Wang Yaxin , Tan Chaolu , Li Yinghao , Meng Fengyan , Du Youwei , Zhang Shuyu , Jiang Wenxin , Feng Naijie , Zhao Liming , Zheng Dianfeng
Published: February 2024

A large number of dead seedlings can occur in saline soils, which seriously affects the large-scale cultivation of rice. This study investigated the effects of plant growth regulators (PGRs) and nitrogen application on seedling growth and salt tolerance (Oryza sativa L.), which is of great significance for agricultural production practices. A conventional rice variety, “Huang Huazhan”, was selected for this study. Non-salt stress treatments included 0% NaCl (CK treatment), CK + 0.05 g N/pot (N treatment), CK + 40 mg·L−1 5-aminolevulinic acid (5-ALA) (A treatment), and CK + 30 mg·L−1 diethylaminoethyl acetate (DTA-6) (D treatment). Salt stress treatments included 0.3% NaCl (S treatment), N + 0.3% NaCl (NS treatment), A + 0.3% NaCl (AS treatment), and D + 0.3% NaCl (DS treatment). When 3 leaves and 1 heart emerged from the soil, plants were sprayed with DTA-6 and 5-ALA, followed by the application of 0.3% NaCl (w/w) to the soil after 24 h. Seedling morphology and photosynthetic indices, as well as carbohydrate metabolism and key enzyme activities, were determined for each treatment. Our results showed that N, A, and D treatments promoted seedling growth, photosynthesis, carbohydrate levels, and the activities of key enzymes involved in carbon metabolism when compared to the CK treatment. The A treatment had the most significant effect, with increases in aboveground dry weight and net photosynthetic rates (Pn) ranging from 17.74% to 41.02% and 3.61% to 32.60%, respectively. Stomatal limiting values (Ls) significantly decreased from 19.17% to 43.02%. Salt stress significantly inhibited seedling growth. NS, AS, and DS treatments alleviated the morphological and physiological damage of salt stress on seedlings when compared to the S treatment. The AS treatment was the most effective in improving seedling morphology, promoting photosynthesis, increasing carbohydrate levels, and key enzyme activities. After AS treatment, increases in aboveground dry weight, net photosynthetic rate, soluble sugar content, total sucrose synthase, and amylase activities were 17.50% to 50.79%, 11.39% to 98.10%, 20.20% to 80.85%, 21.21% to 33.53%, and 22.17% to 34.19%, respectively, when compared to the S treatment. In summary, foliar sprays of 5-ALA, DTA-6, and additional nitrogen fertilizer enhanced rice seedling growth, increased photosynthesis, lowered Ls values, and improved seedling salt tolerance. Spraying two regulators, 5-ALA and DTA-6, quantitatively increased the effect of nitrogen fertilizer, with comparable effects on NaCl stress regulation. This study provides the basis for efficient agricultural production.
Metabolomic Analysis Reveals Association between Decreased Ovarian Reserve and In Vitro Fertilization Outcomes
Authors: An Na , Zhang Min , Zhu Quan-Fei , Chen Yao-Yu , Deng Yan-Ling , Liu Xiao-Ying , Zeng Qiang , Feng Yu-Qi
Published: February 2024

In vitro fertilization (IVF) is a highly effective treatment for infertility; however, it poses challenges for women with decreased ovarian reserve (DOR). Despite the importance of understanding the impact of DOR on IVF outcomes, limited research has explored this relationship, particularly using omics approaches. Hence, we conducted a study to investigate the association between DOR and IVF outcomes, employing a metabolomic approach. We analyzed serum samples from 207 women undergoing IVF treatment, including 89 with DOR and 118 with normal ovarian reserve (NOR). Our findings revealed that DOR was significantly associated with unfavorable IVF outcomes, characterized by a reduced oocyte count, lower embryo quality, and decreased rates of pregnancy and live births. Furthermore, we identified 82 metabolites that displayed significant alterations in DOR patients, impacting diverse metabolic pathways. Notably, a distinct panel of metabolites, including palmitic acid, stearic acid, LysoPC(9:0(CHO)/0:0), PC(18:0/9:0(CHO)), and PC(16:0/9:0(CHO)), exhibited discriminatory power between the DOR and NOR groups, showcasing a strong correlation with IVF outcomes. These findings emphasize the crucial role of metabolomic disruptions in influencing IVF outcomes among women with DOR.
Isolation of Thermophilic Bacteria from Extreme Environments in Northern Chile
Authors: Valenzuela Bernardita , Solís-Cornejo Francisco , Araya Rubén , Zamorano Pedro
Published: February 2024

The northern region of Chile boasts unique geographical features that support the emergence of geothermal effluents, salt lagoons, and coastal creeks. These extreme climate conditions create polyextreme habitats for microorganisms, particularly adapted to survive these harsh environments. These extremophilic microorganisms hold immense potential as a source of hydrolytic enzymes, among other biotechnological applications. In this study, we isolated 15 strains of aerobic thermophilic bacteria (45–70 °C) from sediment samples collected at five different ecological sites, including hot springs, geothermal fields, and lagoons in the Atacama Desert and Andes high planes. Analyses of the 16S rRNA gene sequences of the isolates showed a close genetic similarity (98–100%) with microorganisms of the genera Parageobacillus, Geobacillus, Anoxybacillus, and Aeribacillus. Notably, these thermophiles exhibited significant hydrolytic enzyme activity, particularly amylases, lipases, and proteases. These findings underscore the potential of using these thermophilic bacterial strains as an invaluable source of thermozymes with wide-ranging applications in diverse industries, such as detergent formulations, pharmaceutical processing, and food technology. This research highlights the ecological significance of these extreme environments in the Atacama Desert and Andes high plains, which serve as vital ecological niches housing extremophilic bacteria as a genetic source of relevant thermozymes, promising great potential for innovation in the biotechnology industry.
An Evaluation of the Antibacterial, Antileishmanial, and Cytotoxic Potential of the Secondary Metabolites of Streptomyces sp. ARH (A3)
Authors: de Azevedo Virlanna Larissa Santos , Rosa Fernanda Costa , Dias Leo Ruben Lopes , Batista Lucas Abrantes , Melo Mariana Costa , Sales Luis Alfredo Torres , Branco Abia de Jesus Martins , Araújo Thalison Rômulo Rocha , de Miranda Rita de Cássia Mendonça , Aliança Amanda Silva dos Santos
Published: February 2024

This study aimed to evaluate the antibacterial, leishmanicidal, and cytotoxic potential of metabolites produced by bacteria isolated from rhizosphere soil samples. The bacterium was identified by genome sequencing as Streptomyces kronopolitis. A preliminary screening was carried out for the antimicrobial activity of S. kronopolitis, demonstrating activity against Staphylococcus aureus ATCC 6538, Corynebacterium diphtheriae ATCC 27010, C. diphtheriae ATCC 27012, and Mycobacterium abscessus, with inhibition halos of sizes 25, 36, 29, and 33 mm, respectively. To obtain secondary metabolites, the bacteria were subjected to submerged fermentation, and the metabolites were extracted using the liquid–liquid method with ethyl acetate. There was a similar MIC for M. abscessus and the two strains of C. diphtherium, reaching a concentration of 12.5 µg/mL, while that of S. aureus was 0.048 µg/mL. Assays for leishmanicidal activity and cytotoxicity against HEp-2 cells and red blood cells were performed. The metabolite showed an IC50 of 9.0 ± 0.9 µg/mL and CC50 of 221.2 ± 7.0 µg/mL. This metabolite does not have hemolytic activity and is more selective for parasites than for mammalian cells, with a selectivity index of 24.6. Thus, the studied metabolite may be a strong candidate for the development of less toxic drugs to treat diseases caused by pathogens.
Effects of Different Heavy Metal Stressors on the Endophytic Community Composition and Diversity of Symphytum officinale
Authors: Ma Jing , Chen Dawei , Xu Yifan , Liu Yue , Liu Lele , Huang Jing , Gao Ruochun , Bai Jie , Hou Qinzheng
Published: February 2024

Endophytes play an important role in helping plants resist heavy metal stress. However, little is known about the effects of different heavy metals on the diversity and composition of endophyte communities. In this study, we used 16S and ITS amplicon sequencing to reveal the structure and function of endophytes in Symphytum officinale under different heavy metal stressors. The results showed that the endophytic fungal diversity decreased compared with the control under the different heavy metals stressors, while the diversity of endophytic bacteria showed an increasing trend. The biomarker analysis indicated that Zn and Pb stress led to obvious branches. Specific OTUs analysis showed that there were 1224, 597, and 1004 OTUs specific under Zn, Pb, and Cd stress in the bacterial community and 135, 81, and 110 OTUs specific under Zn, Pb, and Cd stress in the fungal community. The co-occurrence network showed changes in microbial interactions under heavy metal contamination conditions, suggesting that endophytic bacteria play an important role in the resistance of host plants. The Spearman analysis showed that the correlation between endophytic bacteria and endophytic fungi in relation to heavy metal transport exhibited variations. Our results expand the knowledge of the relationships of plant–microbe interactions and offer pivotal information to reveal the role of endophytes under different heavy metal stress conditions.
Progress in Research on the Gut Microflora of the Red Panda (Ailurus fulgens)
Authors: Zhao Xing , Zhang Zejun , Wang Le , Zhang Qian , Kang Liwen , Wang Jia , Long Juejie , Hong Mingsheng
Published: February 2024

Animals can adapt to unique feeding habits through changes in the structure and function of the gut microflora. However, the gut microflora is strongly influenced by the evolutionary relationships between the host, nutritional intake, intake of microorganisms, etc. The red panda (Ailurus fulgens), an herbivorous carnivore, has adapted to consuming bamboo through seasonal foraging strategies and optimization of the composition and function of its gut microflora during long-term evolution. However, to date, studies of the gut bacteria of the red panda have mainly focused on the composition, diversity and function of the gut microflora of captive individuals. There are a lack of studies on how the wild red panda adapts to the consumption of bamboo, which is high in fibre and low in nutrients, through the gut microflora. This paper reviews the technology and methods used in published studies investigating the gut microflora of the red panda, as well as the composition, diversity and function of the identified microbes and the influencing factors. Furthermore, this paper suggests future research directions regarding the methodology employed in analyzing the red panda gut microflora, the interplay between gut microflora and the health of the red panda, the red panda’s adaptation to its gut microflora, and the implications of these studies for the management and conservation of wild red pandas. The goal of this review is to provide a reference for the protection of wild red pandas from the perspective of the gut microflora.
Killing of Plasmodium Sporozoites by Basic Amphipathic α-Helical Fusion Peptides
Authors: Aguirre-Botero Manuela C. , Aliprandini Eduardo , Gladston Anisha , Pacios Olga , Miyazawa Martins Rafael , Poyet Jean-Luc , Amino Rogerio
Published: February 2024

Membranolytic molecules constitute the first line of innate immune defense against pathogenic microorganisms. Plasmodium sporozoites are potentially exposed to these cytotoxic molecules in the hemolymph and salivary glands of mosquitoes, as well as in the skin, blood, and liver of the mammalian host. Here, we show that sporozoites are resistant to bacteriolytic concentration of cecropin B, a cationic amphipathic antimicrobial insect peptide. Intriguingly, anti-tumoral cell-penetrating peptides derived from the anti-apoptotic protein AAC11 killed P. berghei and P. falciparum sporozoites. Using dynamic imaging, we demonstrated that the most cytotoxic peptide, called RT39, did not significantly inhibit the sporozoite motility until the occurrence of a fast permeabilization of the parasite membrane by the peptide. Concomitantly, the cytosolic fluorescent protein constitutively expressed by sporozoites leaked from the treated parasite body while To-Pro 3 and FITC-labeled RT39 internalized, respectively, binding to the nucleic acids and membranes of sporozoites. This led to an increase in the parasite granularity as assessed by flow cytometry. Most permeabilization events started at the parasite’s posterior end, resulting in the appearance of a fluorescent dot in the anterior part of sporozoites. Understanding and exploiting the susceptibility of sporozoites and other plasmodial stages to membranolytic molecules might foster strategies to eliminate the parasite and block its transmission.
FeOx-Modified Ultrafine Platinum Particles Supported on MgFe2O4 with High Catalytic Activity and Promising Stability toward Low-Temperature Oxidation of CO
Authors: Wang Chanchan , Wang Fen , Shi Jianjun
Published: February 2024

Catalytic oxidation is widely recognized as a highly effective approach for eliminating highly toxic CO. The current challenge lies in designing catalysts that possess exceptional low-temperature activity and stability. In this work, we have prepared ultrafine platinum particles of ~1 nm diameter dispersed on a MgFe2O4 support and found that the addition of 3 wt.% FeOx into the 3Pt/MgFe2O4 significantly improves its activity and stability. At an ultra-low temperature of 30 °C, the CO can be totally converted to CO2 over 3FeOx-3Pt/MgFe2O4. High and stable performances of CO-catalytic oxidation can be obtained at 60 °C on 3FeOx-3Pt/MgFe2O4 over 35 min on-stream at WHSV = 30,000 mL/(g·h). Based on a series of characterizations including BET, XRD, ICP, STEM, H2-TPR, XPS, CO-DRIFT, O2-TPD and CO-TPD, it was disclosed that the relatively high activity and stability of 3FeOx-3Pt/MgFe2O4 is due to the fact that the addition of FeOx could facilitate the antioxidant capacity of Pt and oxygen mobility and increase the proportion of adsorbed oxygen species and the amounts of adsorbed CO. These results are helpful in designing Pt-based catalysts exhibiting higher activity and stability at low temperatures for the catalytic oxidation of CO.
A Photoelectrochemical Sensor for the Sensitive Detection of Cysteine Based on Cadmium Sulfide/Tungsten Disulfide Nanocomposites
Authors: Wang Yan , Liu Jiaxin , Lin Fancheng
Published: February 2024

In this work, a CdS-nanoparticle-decorated WS2 nanosheet heterojunction was successfully prepared and first used to modify ITO electrodes for the construction of a novel photoelectrochemical sensor (CdS/WS2/ITO). The thin-film electrode was fabricated by combining electrophoretic deposition with successive ion layer adsorption and reaction techniques. The results indicated that the synthesized heterojunction nanomaterials displayed excellent photoelectrochemical performance which was much better than that of pristine CdS nanoparticles and 2D WS2 nanosheets. Owing to the formation of the surface heterojunction and the effective interfacial electric field, the enhanced separation of photogenerated electron–hole pairs led to a remarkable improvement in the photoelectrochemical activity of CdS/WS2/ITO. This heterojunction architecture can protect CdS against photocorrosion, resulting in a stable photocurrent. Based on the specific recognition between cysteine and CdS/WS2/ITO, through the specificity of Cd-S bonds, a visible-light-driven photoelectrochemical sensor was fabricated for cysteine detection. The novel photoelectrochemical biosensor exhibited outstanding analytical capabilities in detecting cysteine, with an extremely low detection limit of 5.29 nM and excellent selectivity. Hence, CdS-WS2 heterostructure nanocomposites are promising candidates as novel advanced photosensitive materials in the field of photoelectrochemical biosensing.
Highly Sensitive and Stable Multifunctional Self-Powered Triboelectric Sensor Utilizing Mo2CTx/PDMS Composite Film for Pressure Sensing and Non-Contact Sensing
Authors: Fan Jialiang , Wang Chenxing , Wang Bo , Wang Bin , Liu Fangmeng
Published: February 2024

Sensors based on triboelectric nanogenerators (TENGs) are increasingly gaining attention because of their self-powered capabilities and excellent sensing performance. In this work, we report a Mo2CTx-based triboelectric sensor (Mo-TES) consisting of a Mo2CTx/polydimethylsiloxane (PDMS) composite film. The impact of the mass fraction (wt%) and force of Mo2CTx particles on the output performance of Mo-TES was systematically explored. When Mo2CTx particles is 3 wt%, Mo-TES3 achieves an open-circuit voltage of 86.89 V, a short-circuit current of 578.12 nA, and a power density of 12.45 μW/cm2. It also demonstrates the ability to charge capacitors with varying capacitance values. Additionally, the Mo-TES3 demonstrates greater sensitivity than the Mo-TES0 and a faster recovery time of 78 ms. Meanwhile, the Mo-TES3 also demonstrates excellent stability in water washing and antifatigue testing. This demonstrates the effectiveness of Mo-TES as a pressure sensor. Furthermore, leveraging the principle of electrostatic induction, the triboelectric sensor has the potential to achieve non-contact sensing, making it a promising candidate for disease prevention and safety protection.
Polystyrene Nano- and Microplastic Particles Induce an Inflammatory Gene Expression Profile in Rat Neural Stem Cell-Derived Astrocytes In Vitro
Authors: Marcellus Kristen A. , Bugiel Steven , Nunnikhoven Andrée , Curran Ivan , Gill Santokh S.
Published: February 2024

Microplastics are considered an emerging environmental pollutant due to their ubiquitous presence in the environment. However, the potential impact of microplastics on human health warrants further research. Recent studies have reported neurobehavioral and neurotoxic effects in marine and rodent models; however, their impact on the underlying cellular physiology in mammals remains unclear. Herein, we exposed neural stem cells and neural stem cell-derived astrocytes, oligodendrocytes, and neurons to various sizes and concentrations of polystyrene nano- and microplastics. We investigated their cellular uptake, impact on cytotoxicity, and alteration of gene expression through transcriptome profiling. The cell type most affected by decreased viability were astrocytes after 7 days of repeated exposure. Transcriptional analysis showed that 1274 genes were differentially expressed in astrocytes exposed to 500 nm microplastics, but only 531 genes were altered in astrocytes exposed to 50 nm nanoplastics. Both canonical pathway and Kyoto Encyclopedia of Genes and Genomes analysis showed that upregulated pathways were involved in neuroinflammation, innate and adaptive immunity, cell migration, proliferation, extracellular matrix remodeling, and cytoskeleton structures. The downregulated pathways were involved in lipid metabolism, specifically fatty acid oxidation and cholesterol metabolism. Our results show that neural stem cell-derived astrocytes repeatedly exposed to nano- and microplastics for 7 days undergo changes that are hallmarks of astrogliosis.
Polymer Lewis Base for Improving the Charge Transfer in Tin–Lead Mixed Perovskite Solar Cells
Authors: Xing Yanjun , Deng Zhiqiang , Wang Qiuxiang , Xiong Jiaxing , Liu Xiaohui , Huang Like , Zhu Yuejin , Zhang Jing
Published: February 2024

The poor film stability of Sn-Pb mixed perovskite film and the mismatched interface energy levels pose significant challenges in enhancing the efficiency of tin–lead (Sn-Pb) mixed perovskite solar cells. In this study, polyvinylpyrrolidone (PVP) is introduced into the PVK perovskite precursor solution, effectively enhancing the overall stability of the film. This improvement is achieved through the formation of robust coordination bonds between the carbonyl (C=O) in the pyrrole ring and the undercoordinated SnII and PbII, thereby facilitating the passivation of defects. Furthermore, the introduction of PVP inhibits the oxidation of tin (Sn), thereby enhancing the n-type characteristics of the perovskite film. This adjustment in the energy level of the PVK perovskite film proves instrumental in reducing interface energy loss, subsequently improving interface charge transfer and mitigating device recombination. Consequently, perovskite solar cells incorporating PVP achieve an outstanding champion power conversion efficiency (PCE) of 21.31%.
Dynamics of Gut Microbiota and Short-Chain Fatty Acids during a Cycling Grand Tour Are Related to Exercise Performance and Modulated by Dietary Intake
Authors: Fernandez-Sanjurjo Manuel , Fernandez Javier , Martinez-Camblor Pablo , Rodriguez-Alonso Manuel , Ortolano-Rios Raquel , Pinto-Hernandez Paola , Castilla-Silgado Juan , Coto-Vilcapoma Almudena , Ruiz Lorena , Villar Claudio J. , Tomas-Zapico Cristina , Margolles Abelardo , Fernandez-Garcia Benjamin , Iglesias-Gutierrez Eduardo , Lombó Felipe
Published: February 2024

Background: Regular exercise has been described to modify both the diversity and the relative abundance of certain bacterial taxa. To our knowledge, the effect of a cycling stage race, which entails extreme physiological and metabolic demands, on the gut microbiota composition and its metabolic activity has not been analysed. Objective: The aim of this cohort study was to analyse the dynamics of faecal microbiota composition and short-chain fatty acids (SCFAs) content of professional cyclists over a Grand Tour and their relationship with performance and dietary intake. Methods: 16 professional cyclists competing in La Vuelta 2019 were recruited. Faecal samples were collected at four time points: the day before the first stage (A); after 9 stages (B); after 15 stages (C); and on the last stage (D). Faecal microbiota populations and SCFA content were analysed using 16S rRNA sequencing and gas chromatography, respectively. A principal component analysis (PCA) followed by Generalised Estimating Equation (GEE) models were carried out to explore the dynamics of microbiota and SCFAs and their relationship with performance. Results: Bifidobacteriaceae, Coriobacteriaceae, Erysipelotrichaceae, and Sutterellaceae dynamics showed a strong final performance predictive value (r = 0.83, ranking, and r = 0.81, accumulated time). Positive correlations were observed between Coriobacteriaceae with acetate (r = 0.530) and isovalerate (r = 0.664) and between Bifidobacteriaceae with isobutyrate (r = 0.682). No relationship was observed between SCFAs and performance. The abundance of Erysipelotrichaceae at the beginning of La Vuelta was directly related to the previous intake of complex-carbohydrate-rich foods (r = 0.956), while during the competition, the abundance of Bifidobacteriaceae was negatively affected by the intake of simple carbohydrates from supplements (r = −0.650). Conclusions: An ecological perspective represents more realistically the relationship between gut microbiota composition and performance compared to single-taxon approaches. The composition and periodisation of diet and supplementation during a Grand Tour, particularly carbohydrates, could be designed to modulate gut microbiota composition to allow better performance.
Concomitant Syndromic Diagnosis of Mpox and Other Vesicular Viruses in Patients with Skin and Genital Lesions
Authors: Valli Maria Beatrice , Vulcano Antonella , Rueca Martina , Matusali Giulia , Mazzotta Valentina , Nicastri Emanuele , Girardi Enrico , Fontana Carla , Antinori Andrea , Maggi Fabrizio
Published: February 2024

The recent multi-country outbreak of the zoonotic monkeypox virus (MPXV) infection in humans without an epidemiological link with endemic areas has raised concerns about the route of transmission. Since the infection spread largely among men who have sex with men who, in most cases, presented primary lesions of the genital and oral mucosa, sexual transmission has been proposed. In the present study, we retrospectively evaluated specimens of vesicular lesions collected from the skin and genital tract of 35 patients (23 positive and 12 negative) presenting at our Institute for monkeypox (mpox) diagnosis by using a novel molecular syndromic vesicular virus panel (VVP) assay. All MPXV-positive samples but one was confirmed; however, the viral syndromic analysis revealed that 8.6% of them were coinfected with one or more viruses, and 17% had at least a virus different from the MPXV. The percentage of coinfections increased to more than 25% when nonviral pathogens, such as gonorrhea and syphilis, were also considered. These results show the usefulness of syndromic diagnosis in cases where MPXV is suspected (and vice versa) and at the same time highlight that the broader screening of sexually transmitted infections in the population with high-risk sexual behavior is critical to ensure a complete etiology and appropriate treatment.
A Tribute to a Visionary Scientist—John R. David—Richard Pearson Strong, Professor of Tropical Public Health, Emeritus, Harvard
Authors: Shaw Jeffrey J. , Satoskar Abhay
Published: February 2024

There are rare individuals whose insatiable curiosity and boundless intellect propel them into multiple frontiers of science, leaving an indelible mark on the fields that they venture into [...]
Human Papillomavirus (HPV) Infection and Risk Behavior in Vaccinated and Non-Vaccinated Paraguayan Young Women
Authors: Bobadilla María Liz , Villagra Verónica , Castro Héctor , von Horoch Marta , Araya Soraya , Deluca Gerardo , de Paula Vanessa Salete
Published: February 2024

Cervical cancer is a global health concern and ranks fourth among the most prevalent cancers in women worldwide. Human papillomavirus (HPV) infection is a known precursor of cervical cancer and preventive measures include prophylactic vaccines. This study focused on sexually active Paraguayan women aged 18–25 years, exploring the intersection of HPV vaccination and sexual behavior. Among 254 participants, 40.9% received the Gardasil-4 vaccine, with no significant differences in sexual behavior between the vaccinated and unvaccinated sexually active groups. However, a notable decrease in the prevalence of HPV among the vaccinated women highlights the efficacy of this vaccine in reducing infections. The prevalence of any HPV type was 37.5% in vaccinated participants compared to 56.7% in unvaccinated participants (p = 0.0026). High-risk HPV types showed a significant difference, with a prevalence of 26.0% in vaccinated women compared with 52.7% in unvaccinated women (p < 0.001). Although a potential decline in genital warts was observed among the vaccinated individuals, statistical significance (p = 0.0564) was not reached. Despite the challenges in achieving high vaccination coverage, the observed reduction in HPV prevalence underscores the importance of ongoing monitoring, healthcare professional recommendations, and comprehensive risk management. These findings contribute to dispelling concerns about HPV vaccination influencing sexual behavior, advocating further large-scale research to explore the impact of vaccines on various HPV types and potential cross-protection.
Submilliwatt Silicon Nitride Thermo-Optic Modulator Operating at 532 nm
Authors: Wu Zhaoyang , Lin Shuqing , Yu Siyuan , Zhang Yanfeng
Published: February 2024

Optical phase control is essential for optical beam steering applications. The silicon nitride thermo-optic modulator generally suffers from high electrical power consumption. Microresonator and multipass structures could reduce the electrical power consumption of silicon nitride thermo-optic modulators, with the drawback of a narrow operating bandwidth and high insertion loss. We demonstrate a single-pass silicon nitride thermo-optic phase modulator at 532 nm with low insertion loss and low power consumption, achieving a π phase shift power consumption down to 0.63 mW in a Mach–Zehnder switch. The rise and fall time are around 1.07 ms and 0.67 ms, respectively.
Generation of Different Mode-Locked States in Nonlinear Multimodal Interference-Based Fiber Lasers
Authors: Deng Gang , Yang Qiaochu , Du Silun , Chen Bowen , Li Baoqun , Wang Tianshu
Published: February 2024

A novel mode-locking method based on nonlinear multimode interference (NLMI) using a distributed large-core (105 μm) graded-index multimode fiber (GIMF)-based saturable absorber (SA) capable of generating four pulse modes is proposed. The distributed SA geometry consists of two GIMFs located at different positions in the resonant cavity. The coupling and joint operation not only facilitate resistance to pulse fragmentation but also provide a sophisticated and widely tunable transmission with saturable and reverse saturable absorption phenomena. Based on this, dissipative soliton (DS), dissipative soliton resonance (DSR), wedge-shaped, and staircase pulses are achieved without additional filters. The DS has accessible output power, pulse energy, bandwidth, and duration of up to 15.33 mW, 2.02 nJ, 22.63 nm, and ~1.68 ps. The DSR has an achievable pulse duration and energy of ~32.39 ns, 30.3 nJ. The dispersion range that allows DS operation is studied, and the dynamics of the evolution from DS to DSR are observed. The versatility, flexibility, and simplicity of the SA device, combined with the possibility of scaling the pulse energy, make it highly attractive for ultrafast optics and nonlinear dynamics.
Emerging Trends in Ant–Pollinator Conflict in Extrafloral Nectary-Bearing Plants
Authors: Calixto Eduardo , de Oliveira Pimenta Isabela , Lange Denise , Marquis Robert , Torezan-Silingardi Helena , Del-Claro Kleber
Published: February 2024

The net outcomes of mutualisms are mediated by the trade-offs between the costs and benefits provided by both partners. Our review proposes the existence of a trade-off in ant protection mutualisms between the benefits generated by the ants’ protection against the attack of herbivores and the losses caused by the disruption of pollination processes, which are commonly not quantified. This trade-off has important implications for understanding the evolution of extrafloral nectaries (EFNs), an adaptation that has repeatedly evolved throughout the flowering plant clade. We propose that the outcome of this trade-off is contingent on the specific traits of the organisms involved. We provide evidence that the protective mutualisms between ants and plants mediated by EFNs have optimal protective ant partners, represented by the optimum point of the balance between positive effects on plant protection and negative effects on pollination process. Our review also provides important details about a potential synergism of EFN functionality; that is, these structures can attract ants to protect against herbivores and/or distract them from flowers so as not to disrupt pollination processes. Finally, we argue that generalizations regarding how ants impact plants should be made with caution since ants’ effects on plants vary with the identity of the ant species in their overall net outcome.