bluuebunny/crossref_metadata_2025 huggingface dataset:
Protein Hydrolysates and Mo-Biofortification Interactively Modulate Plant Performance and Quality of ‘Canasta’ Lettuce Grown in a Protected Environment
Authors: Sabatino Leo , Consentino Beppe Benedetto , Rouphael Youssef , De Pasquale Claudio , Iapichino Giovanni , D’Anna Fabio , La Bella Salvatore
Published: May 2021

Since the use of protein hydrolysates (PHs) enhances overall plant performance and quality of vegetables, they might be considered as a toll to face a number of concerns essentially associated to the growing request of premium quality foodstuff realized in agreement with eco-friendly agriculture practices. Molybdenum (Mo) is considered a fundamental trace element for human body. Thus, its shortage determines several disorders mainly related to neurological lesion and esophageal cancer. Biofortification of fruiting and leafy vegetables is a promising tool to prevent Mo deficiency in the human diet. The current study was carried out to assess the interactive effect of plant-derived PHs and Mo dosage (0.0, 0.5, 3.0, and 6.0 µmol L−1) on yield, morphology, nutritional and functional features, and nitrogen indices of ‘Canasta’ lettuce. Head fresh weight (HFW), head height (HH), ascorbic acid, K, Mg, total chlorophyll, as well as nitrogen use efficiency (NUE) index were positively correlated to PHs application. Furthermore, ascorbic acid and total chlorophyll were also improved by Mo supply. A great improvement in terms of soluble solid content (SSC), total sugars, total phenolic, carotenoids, Mo and N concentrations, nitrogen uptake efficiency (UE), and nitrogen physiological efficiency (PUE) indices was recorded when PHs application was combined with the highest Mo dosage (6.0 µmol L−1). Consequently, our results suggest that Mo-biofortification and PHs application can positively modulate ‘Canasta’ lettuce plant performance and quality.
Effects of Autotoxicity on Alfalfa (Medicago sativa): Seed Germination, Oxidative Damage and Lipid Peroxidation of Seedlings
Authors: Zhang Xiao-Yan , Shi Shang-Li , Li Xiao-Long , Li Chang-Ning , Zhang Cui-Mei , A Yun , Kang Wen-Juan , Yin Guo-Li
Published: May 2021

Alfalfa (Medicago sativa) is a highly valuable perennial forage legume that suffers from autotoxicity, which decreases plant resistance, reduces soil fertility, causes serious soil-borne diseases, and promotes ecological imbalance. We evaluated the effects of autotoxicity on the seed germination of 22 alfalfa varieties, and then elucidated the oxidative damage and lipid peroxidation in two alfalfa varieties with contrasting autotoxicity tolerances. The technique for order of preference by similarity to ideal solution (TOPSIS) method was used to rank the germination of the 22 alfalfa varieties when exposed to six autotoxic concentrations (0, 0.025, 0.075, 0.125, 0.175, and 0.225 g∙mL−1). We found WL656HQ and 3105C to be autotoxicity-tolerant and autotoxicity-sensitive varieties, respectively. The germination index mainly affects the comprehensive allelopathic index of WL656HQ and 3105C, which were the simple vigor index and radicle length according to the random forest model, respectively. 3105C eliminates reactive oxygen species (ROS) via antioxidant enzymes and antioxidants under T1 (0.025 g∙mL−1), but the oxidative stress system and the oxidative scavenging system cannot maintain the balance under T2 (0.125 g∙mL−1), causing oxidative bursts. In comparison, WL656HQ used its oxidative scavenging system (peroxidase (POD), ascorbate peroxidase (APX), and glutathione reductase (GR)) to maintain its redox dynamic balance by removing excess ROS at all concentrations. In conclusion, the positive and negative indicators of autotoxicity for the two varieties were ascorbate (ASA) and hydroxyl free radicals (OH•), and proline (Pro) and dehydroascrobate (DHA), respectively. The most sensitive autotoxic concentrations of 3105C and WL656HQ were T2 (0.125 g∙mL−1) and T1 (0.025 g∙mL−1), respectively.
Use of New BTH Derivative as Supplement or Substitute of Standard Fungicidal Program in Strawberry Cultivation
Authors: Spychalski Maciej , Kukawka Rafal , Krzesiński Włodzimierz , Spiżewski Tomasz , Michalecka Monika , Poniatowska Anna , Puławska Joanna , Mieszczakowska-Frąc Monika , Panasiewicz Katarzyna , Kocira Anna , Smiglak Marcin
Published: May 2021

Triggering the plant resistance induction phenomenon by chemical compounds, for example acibenzolar-S-methyl ester, has been known and described in scientific literature. Other benzothadiazole derivatives have been also described; however, their properties have not been sufficiently studied. The tested substance, N-methyl-N-methoxyamide-7-carboxybenzo(1.2.3)thiadiazole (BTHWA), is an amide derivative of benzothiadiazole, showing a stimulating effect on plant growth, apart from its plant resistance inducing activity. This article presents the impact of BTHWA, used solo and in the program with fungicides, on the strawberry plants development, fruits health, yield, and quality parameters of the crop. The results show that the combined use of BTHWA and fungicides had a positive impact on the plants health and fruit health and nutraceutical and nutritional composition of compounds when compared to the results obtained when strawberries were treated only with the BTHWA or the fungicide. As a result of BTHWA use, the partitioning of assimilates has changed, which directly translated into the results of the conducted experiments. A reduction in the respiration of the fruit during storage was also observed, possibly due to a reduced disease infestation and a lower dry matter content in the fruit. A correlation between the parameters determined during the experiment was found. The BTHWA mode of action was evidenced to be beneficial to strawberry plants and fruit.
Comparison of the Therapeutic Effect of Treatment with Antibiotics or Nutraceuticals on Clinical Activity and the Fecal Microbiome of Dogs with Acute Diarrhea
Authors: Pignataro Giulia , Di Prinzio Roberta , Crisi Paolo Emidio , Belà Benedetta , Fusaro Isa , Trevisan Carlo , De Acetis Luigi , Gramenzi Alessandro
Published: May 2021

Dogs with acute diarrhea are often presented to clinical practice and, although this generally represents a self-limiting condition, antibiotics are still frequently used as treatment. The aim of this study was to evaluate the effects in dogs with acute non-hemorrhagic diarrhea of the administration of an antibiotic combination in comparison to a nutraceutical product. Thirty dogs were enrolled and randomly assigned to two groups: 15 dogs (group A) received a nutraceutical commercial product while 15 dogs (group B) received an antimicrobial combination of metronidazole and spiramycin. For each dog, the Canine Acute Diarrhea Severity Index, the fecal microbiota and the Dysbiosis Index were assessed. Both stool consistency and frequency decreased on day 2 in the dogs of group A compared to baseline, while in group B, these parameters significantly decreased at days 3 and 4. The global concern for rising antibiotic resistance associated with indiscriminate use of antimicrobials, in both humans and animals, suggests the necessity of avoiding empirical and injudicious use of these molecules in diarrheic dogs. These results suggest that the nutraceutical treatment had a similar clinical effect compared to the antibiotic formulation, representing a valid antibiotic-sparing therapeutic approach in canine acute diarrhea.
Oestrus Analysis of Sows Based on Bionic Boars and Machine Vision Technology
Authors: Lei Kaidong , Zong Chao , Du Xiaodong , Teng Guanghui , Feng Feiqi
Published: May 2021

This study proposes a method and device for the intelligent mobile monitoring of oestrus on a sow farm, applied in the field of sow production. A bionic boar model that imitates the sounds, smells, and touch of real boars was built to detect the oestrus of sows after weaning. Machine vision technology was used to identify the interactive behaviour between empty sows and bionic boars and to establish deep belief network (DBN), sparse autoencoder (SAE), and support vector machine (SVM) models, and the resulting recognition accuracy rates were 96.12%, 98.25%, and 90.00%, respectively. The interaction times and frequencies between the sow and the bionic boar and the static behaviours of both ears during heat were further analysed. The results show that there is a strong correlation between the duration of contact between the oestrus sow and the bionic boar and the static behaviours of both ears. The average contact duration between the sows in oestrus and the bionic boars was 29.7 s/3 min, and the average duration in which the ears of the oestrus sows remained static was 41.3 s/3 min. The interactions between the sow and the bionic boar were used as the basis for judging the sow’s oestrus states. In contrast with the methods of other studies, the proposed innovative design for recyclable bionic boars can be used to check emotions, and machine vision technology can be used to quickly identify oestrus behaviours. This approach can more accurately obtain the oestrus duration of a sow and provide a scientific reference for a sow’s conception time.
Effects of Dietary Rapeseed Meal on Growth Performance, Carcass Traits, Serum Parameters, and Intestinal Development of Geese
Authors: Fu Zhenming , Su Guoqiang , Yang Haiming , Sun Qingyu , Zhong Tao , Wang Zhiyue
Published: May 2021

The use of inexpensive nonconventional feed materials, such as rapeseed meal (RSM), could help alleviate the shortage of feed materials in the poultry industry. This study was to investigate the effects of dietary double-low RSM on growth performance, carcass traits, serum parameters, and intestinal development of geese. A total of 270 healthy 35-day-old male Jiangnan White geese were randomly divided into five treatments, with six replicate pens of nine geese each. The geese were fed five isonitrogenous and isocaloric diets containing 0%, 4%, 8%, 12%, and 16% RSM replacing dietary soybean meal for 35 days. At 35, 49, and 70 d, the BW and feed intake were recorded. All Samples were collected at 70 d of age. The results showed that dietary RSM up to 16% did not affect the BW, ADFI, ADG, and feed/gain ratio (F/G) during 35 to 49 d, 49 to 70 d, and 35 to 70 d periods (p > 0.05). At 70 d, no difference was observed in carcass yield or serum biochemical parameters among groups (p > 0.05). Dietary 12% and 16% RSM significantly increased the concentration of serum GH compared with 0%, 4%, 8% groups (p < 0.01), but serum TSH, T3 and T4 were unaffected (p > 0.05). The relative weights of heart, liver, spleen, proventriculus, gizzard, and small intestine were similar among groups (p > 0.05). However, the geese fed dietary 16% RSM had greater bursa of Fabricius than geese in the 8% group (p < 0.05). Intestinal morphology was unaffected by treatments (p > 0.05). According to the findings, dietary RSM up to 16% can be used in geese diets without impact on production performance.
Investigation of Early Supplementation of Nucleotides on the Intestinal Maturation of Weaned Piglets
Authors: Correa Federico , Luise Diana , Archetti Ivonne , Bosi Paolo , Trevisi Paolo
Published: May 2021

Nucleotides are essential for the development of the gastrointestinal tract and immune function, but their intake with milk by piglets could be insufficient. The effect of nucleotides on growth and health was tested on 98 piglets divided into two groups: NU, orally administrated with 4 mL of a nucleotide-based product (SwineMOD®) at 10, 15, 18, 21, 27 days, or not (CO). Blood and feces were sampled at weaning (26 d, T1), and at 38 d (T2). Per each group and time-point, eight piglets were slaughtered and jejunal Peyer’s patches (JPPs) were collected. NU increased hemoglobin content and hematocrit, but not growth. At weaning, the NU fecal microbiota was characterized by the abundance of Campylobacteraceae, more typical of the growing phase, compared to CO, with a greater abundance of Streptococcaceae. For the blood transcriptome, an initial greater inflammatory activation was seen in CO, while at T2, NU enriched gene sets related to erythropoiesis. The activation of gene groups ranging from epigenetic response to transcriptional regulation evidenced an intense proliferative activity in NU JPPs. NU supplementation did not influence the growth performance of piglets but could have expressed a positive effect on pig microbiota anticipating its maturation at weaning. This immunostimulant activity in the JPPs could moderate the inflammation in the immediate pre-weaning.
Effect of Dietary Mineral Content and Phytase Dose on Nutrient Utilization, Performance, Egg Traits and Bone Mineralization in Laying Hens from 22 to 31 Weeks of Age
Authors: Javadi Mehran , Pascual Juan José , Cambra-López María , Macías-Vidal Judit , Donadeu Andrés , Dupuy Javier , Carpintero Laura , Ferrer Pablo , Cerisuelo Alba
Published: May 2021

A total of 192 laying hens were used to evaluate the effect of dietary mineral content and phytase dose on nutrient utilization, egg production and quality and bone mineralization of young laying hens. Four dietary treatments were studied: PC, positive control with no added phytase, 4.07% Ca and 0.61% P; NC, negative control with no added phytase, 2.97% Ca and 0.37% P; and P500 and P1000, where NC diet was supplemented with phytase at 500 and 1000 FTU/kg, respectively. Hens’ performance and egg traits were controlled from 22 to 31 weeks of age. Coefficients of total tract apparent digestibility (CTTAD) of nutrients were determined at 25 and 31 weeks of age. Apparent ileal digestibility (AID) and blood content of Ca and P, as well as bone traits, were determined at 31 weeks of age. Ca and P retention was higher in birds on PC diet at 25 weeks, but not at 31 weeks of age compared to those on NC diet (p < 0.05). P1000 birds had the highest CTTAD values for dry and organic matter at both ages (p < 0.001). CTTAD of Ca was significantly higher in P1000 diet than in NC diet at 31 weeks of age (p < 0.001). Birds fed with P500 diet at 25 weeks of age and P1000 at 31 weeks of age showed higher CTTAD and retention of P, but lower excretion of P than those fed NC diet (p < 0.05). Phytase inclusion linearly increased AID of dry matter and P (p < 0.001). P500 hens fed had the greatest body weight at the end of the trial (p < 0.05) and P1000 birds had the best feed conversion ratio (p < 0.05). Fowl fed a PC diet produced eggs with higher shell thickness and yolk color than those fed on NC diet (p < 0.05). Phytase inclusion linearly increased the yolk color (p < 0.05). Tibia of laying hens fed with PC had significantly higher ash content than those on NC diet (p < 0.05), and birds fed with P1000 presented intermediate values. It can be concluded that it would be advisable to increase the dose of phytase in the feed of laying hens to obtain long-term benefits.
The Antioxidant Carrichtera annua DC. Ethanolic Extract Counteracts Cisplatin Triggered Hepatic and Renal Toxicities
Authors: Eltamany Enas E. , Elhady Sameh S. , Nafie Mohamed S. , Ahmed Haidy A. , Abo-Elmatty Dina M. , Ahmed Safwat A. , Badr Jihan M. , Abdel-Hamed Asmaa R.
Published: May 2021

Cisplatin is a powerful anti-neoplastic drug that displays multi-organ toxicity, especially to the liver and kidneys. Consumption of phytomedicines is a promising strategy to overcome the side effects of chemotherapy. Carrichtera annua extract proved to possess potent antioxidant activity. Its protective potential against cisplatin-induced hepato–nephrotoxicity was scrutinized. Moreover, a phytochemical study was conducted on C. annua ethyl acetate fraction which led to the isolation of five known phenolic compounds. Structure determination was achieved utilizing 1H- and 13C-NMR spectral analyses. The isolated phytochemicals were trans-ferulic acid (1), kaempferol (2), p-coumaric acid (3), luteolin (4) and quercetin (5). Regarding our biological study, C. annua has improved liver and kidney deteriorated functions caused by cisplatin administration and attenuated the histopathological injury in their tissues. Serum levels of ALT, AST, blood urea nitrogen and creatinine were significantly decreased. C. annua has modulated the oxidative stress mediated by cisplatin as it lowered MDA levels while enhanced reduced-GSH concentrations. More importantly, the plant has alleviated cisplatin triggered inflammation, apoptosis via reduction of INFγ, IL-1β and caspase-3 production. Moreover, mitochondrial injury has been ameliorated as remarkable increase of mtDNA was noted. Furthermore, the MTT assay proved the combination of cisplatin—C. annua extract led to growth inhibition of MCF-7 cells in a notable additive way. Additionally, we have investigated the binding affinity of C. annua constituents with caspase-3 and IFN-γ proteins using molecular simulation. All the isolated compounds exhibited good binding affinities toward the target proteins where quercetin possessed the most auspicious caspase-3 and IFN-γ inhibition activities. Our results put forward that C. annua is a promising candidate to counteract chemotherapy side effects and the observed activity could be attributed to the synergism between its phytochemicals.
Wind-Resistant Capacity Modeling for Electric Transmission Line Towers Using Kriging Surrogates and Its Application to Structural Fragility
Authors: Cai Yunzhu , Wan Jiawei
Published: May 2021

Wind loading on a transmission tower structure is jointly influenced by the wind field, structural parameters, and the geo-spatial configuration of the transmission line. Considering the multi-parametric effect, this paper aims at developing a limit capacity model for transmission towers under strong winds. To this end, the limit capacity of the tower is expressed via two equivalent means: one is the limit wind speed as a function of the wind angle of attack and the span of transmission line; the other is a limit capacity surface with three fundamental wind load components as the principal axes. An adaptive kriging surrogate modeling is constructed to approximate the function/surface with structural uncertainties considered. The performance of the surrogate model is improved by adding support points and then evaluated by the overall accuracy validation and local error check. A numerical example demonstrating the feasibility of the surrogate modeling for the limit capacity of the transmission tower under winds is presented. Finally, a fragility assessment concerning a practical transmission line and towers subjected to typhoons is accomplished using the established limit capacity model of the tower.
A New Methodology for Assessing the Interaction between the Mediterranean Olive Agro-Forest and the Atmospheric Surface Boundary Layer
Authors: Jiménez-Portaz María , Clavero María , Losada Miguel Ángel
Published: May 2021

Historically, the olive grove has been one of the most emblematic ecosystems in Mediterranean countries. Currently, in Andalusia, Spain, the land under olive grove cultivation exceeds 1.5 million hectares, approximately 17% of the regional surface. Its exploitation has traditionally been based on the use of the available land and heterogeneous plantations, with different species adapted to southern Mediterranean climatic conditions, and to the management of the traditional olive cultivation culture. The objective of this work is to characterize the mechanical behavior of the atmospheric surface boundary layer (SBL) (under neutral stability) interacting with different olive grove configurations. Experimental tests were carried out in the Boundary Layer Wind Tunnel (BLWT) of the Andalusian Institute for Earth System Research (IISTA), University of Granada. Three representative configurations of olive groves under neutral atmospheric conditions were tested. The wind flow time series were recorded at several distances and heights downwind the olive plantation models with a cross hot wire anemometry system. Herein, this paper shows the airflow streamwise, including the mean flow and the turbulent characteristics. The spatial variability of these two mechanical magnitudes depends on, among others, the size, the agro-forest length, the layout of the tree rows, the porosity, the tree height, the crown shape and the surface vegetation cover. The aerodynamic diameter and Reynolds number for each agro-forest management unit are proposed as representative variables of the system response, as these could be related to olive grove management. The plantation, in turn, conforms to a windbreak, which affects the microclimate and benefits the elements of the ecosystem. Detailed knowledge of these variables and the interaction between the ecosystem and the atmosphere is relevant to optimize the resources management, land use and sustainability of the overall crop. Thus, this paper presents preliminary work to relate atmospheric variables to environmental variables, some of which could be humidity, erosion, evapotranspiration or pollen dispersion.
Local Analysis of Air Quality Changes in the Community of Madrid before and during the COVID-19 Induced Lockdown
Authors: Betancourt-Odio Manuel Alejandro , Martínez-de-Ibarreta Carlos , Budría-Rodríguez Santiago , Wirth Eszter
Published: May 2021

This paper examines the effect of the COVID-19 induced lockdown upon six pollutants, CO, NO, NO2, PM10, PM2.5, and O3, in the Spanish community of Madrid. The paper relies on clustering methods and multiple regression techniques to control for a battery of potential confounding factors. The results show that the nationwide lockdown, decreed on 13 March by the Spanish government, exerted a statistically significant effect upon most pollution indicators. The estimates range from approximately −82% (NO and NO2) to −3% (CO). Reversely, the COVID-19 induced lockdown raised O3 levels by an average of 20%. By using data from 43 stations spread out among the region, the paper provides a local level analysis. This analysis reveals substantial differences across areas and across pollutants. This observation indicates that any successful approach to improve air quality in the region must be multidimensional.
Estimating Particulate Matter Emission from Dust Sources Using ZY-3 Data and GIS Technology—A Case Study in Zhengzhou City, China
Authors: Yang Huan , Song Xuan , Du Liping , Zhang Qi , Cui Jian , Yin Shasha
Published: May 2021

With the rapid development of the social economy in China, numerous Chinese cities are facing high levels of particulate matter (PM) pollution problems. In this study, high-resolution ZY-3 images and GIS techniques were used to establish the emission inventory of total suspended particle (TSP), particulate matter 10 (PM10) and particulate matter 2.5 (PM2.5) from fugitive dust sources in May 2016, and a spatial grid of 3 km × 3 km resolution was established to demonstrate the spatial distribution of PM emission. Results showed that the total emissions of TSP, PM10 and PM2.5 in Zhengzhou city were 237.5 kt·a−1, 103.7 kt·a−1 and 22.4 kt·a−1, respectively. Construction dust source was the main fugitive dust emission source in Zhengzhou city—the TSP, PM10 and PM2.5 emission of which account for 76.42%, 89.68% and 88.39%, respectively, of the total emission, followed by road dust source and soil dust source. PM emission was higher in Zhongyuan, Huiji, Jinshui and Zhengdong New District, while Zhongmou, Xingyang, Dengfeng and other remote areas had low PM emissions. Compared to other Chinese cities or regions, the PM emission from the construction dust source was at a high level in Zhengzhou city, while the PM emissions from the soil dust source and road dust source were at moderate levels.
Heat Shock Factor 1 as a Prognostic and Diagnostic Biomarker of Gastric Cancer
Authors: Kim Woong , Kim Seok-Jun
Published: May 2021

Identification of effective prognostic and diagnostic biomarkers is needed to improve the diagnosis and treatment of gastric cancer. Early detection of gastric cancer through diagnostic markers can help establish effective treatments. Heat shock factor 1 (HSF1), presented in this review, is known to be regulated by a broad range of transcription factors, including those characterized in various malignant tumors, including gastric cancer. Particularly, it has been demonstrated that HSF1 regulation in various cancers is correlated with different processes, such as cell death, proliferation, and metastasis. Due to the effect of HSF1 on the initiation, development, and progression of various tumors, it is considered as an important gene for understanding and treating tumors. Additionally, HSF1 exhibits high expression in various cancers, and its high expression adversely affects the prognosis of various cancer patients, thereby suggesting that it can be used as a novel, predictive, prognostic, and diagnostic biomarker for gastric cancer. In this review, we discuss the literature accumulated in recent years, which suggests that there is a correlation between the expression of HSF1 and prognosis of gastric cancer patients through public data. Consequently, this evidence also indicates that HSF1 can be established as a powerful biomarker for the prognosis and diagnosis of gastric cancer.
Hemostatic and Tissue Regeneration Performance of Novel Electrospun Chitosan-Based Materials
Authors: Deineka Volodymyr , Sulaieva Oksana , Pernakov Mykola , Korniienko Viktoriia , Husak Yevheniia , Yanovska Anna , Yusupova Aziza , Tkachenko Yuliia , Kalinkevich Oksana , Zlatska Alena , Pogorielov Maksym
Published: May 2021

The application of chitosan (Ch) as a promising biopolymer with hemostatic properties and high biocompatibility is limited due to its prolonged degradation time, which, in turn, slows the repair process. In the present research, we aimed to develop new technologies to reduce the biodegradation time of Ch-based materials for hemostatic application. This study was undertaken to assess the biocompatibility and hemostatic and tissue-regeneration performance of Ch-PEO-copolymer prepared by electrospinning technique. Chitosan electrospinning membranes (ChEsM) were made from Ch and polyethylene oxide (PEO) powders for rich high-porous material with sufficient hemostatic parameters. The structure, porosity, density, antibacterial properties, in vitro degradation and biocompatibility of ChEsM were evaluated and compared to the conventional Ch sponge (ChSp). In addition, the hemostatic and bioactive performance of both materials were examined in vivo, using the liver-bleeding model in rats. A penetrating punch biopsy of the left liver lobe was performed to simulate bleeding from a non-compressible irregular wound. Appropriately shaped ChSp or ChEsM were applied to tissue lesions. Electrospinning allows us to produce high-porous membranes with relevant ChSp degradation and swelling properties. Both materials demonstrated high biocompatibility and hemostatic effectiveness in vitro. However, the antibacterial properties of ChEsM were not as good when compared to the ChSp. In vivo studies confirmed superior ChEsM biocompatibility and sufficient hemostatic performance, with tight interplay with host cells and tissues. The in vivo model showed a higher biodegradation rate of ChEsM and advanced liver repair.
Bile Acids Activated Receptors in Inflammatory Bowel Disease
Authors: Biagioli Michele , Marchianò Silvia , Carino Adriana , Di Giorgio Cristina , Santucci Luca , Distrutti Eleonora , Fiorucci Stefano
Published: May 2021

Once known exclusively for their role in nutrients absorption, bile acids have emerged as signaling molecules, generated from cholesterol breakdown, acting on several immune cells by activating a variety of receptors including the G protein-coupled bile acid receptor 1 (GPABR1 or TGR5), the Farnesoid-X-receptor (FXR) and, as recently discovered, the retinoid-related orphan receptors (ROR)γt. GPBAR1, FXR, and RORγt are highly expressed in cells of the innate and adaptive immune system (i.e., dendritic cells (DCs), macrophages, innate lymphoid 3 cells (ILC3s), and T helper 17 (Th17) lymphocytes) and plays an important role in regulating intestinal and liver immunity, highlighting a role for various bile acid species in regulating immune responses to intestinal microbial antigens. While primary bile acids are generated from the cholesterol breakdown secondary bile acids, the GPBAR1 ligands, and oxo-bile acids derivatives, the RORγt ligands, are generated by the intestinal microbiota, highlighting the potential of these bile acids in mediating the chemical communication between the intestinal microbiota and the host. Changes in intestinal microbiota, dysbiosis, alter the composition of the bile acid pool, promoting the activation of the immune system and development of chronic inflammation. In this review, we focus on the molecular mechanisms by which an altered bile acid signaling promotes intestinal inflammation.
Cancer Salt Nostalgia
Authors: Allu Aashish S. , Tiriveedhi Venkataswarup
Published: May 2021

High-salt (sodium chloride) diets have been strongly associated with disease states and poor health outcomes. Traditionally, the impact of salt intake is primarily studied in cardiovascular diseases, hypertension and renal diseases; however, recently there has been increasing evidence demonstrating the role of salt in autoimmune diseases. Salt has been shown to modulate the inflammatory activation of immune cells leading to chronic inflammation-related ailments. To date, there is minimal evidence showing a direct correlation of salt with cancer incidence and/or cancer-related adverse clinical outcomes. In this review article, we will discuss the recent understanding of the molecular role of salt, and elucidate the apparent double-edged sword nature of the relationship between salt and cancer progression.
SARS-CoV-2 Myocarditis in a High School Athlete after COVID-19 and Its Implications for Clearance for Sports
Authors: Das Bibhuti
Published: May 2021

This case report describes a high school athlete with palpitation, myalgia, fatigue, and dyspnea on exertion after SARS-CoV-2 infection with evidence of myocarditis by cardiac magnetic resonance (CMR), but echocardiography and troponin were normal. This case is unusual as the standard cardiac tests recommended by the American Heart Association for sports clearance, including ECG, echocardiography, and cardiac biomarkers, were normal. Still, she continued to be symptomatic after mild COVID-19. The CMR was performed to evaluate her unexplained palpitation and showed patchy myocardial edema two months after her initial SARS-CoV-2 infection. In this case, the diagnosis of myocardial involvement would be missed by normal echocardiograms and cardiac bio-markers without CMR. Because acute myocarditis is a risk factor for sudden death in competitive athletes, pediatric cardiologists should consider performing additional tests such as cardiac MRI in symptomatic COVID-19 patients, even if cardiac biomarkers and echocardiograms are normal.
Calprotectin: An Ignored Biomarker of Neutrophilia in Pediatric Respiratory Diseases
Authors: Chatziparasidis Grigorios , Kantar Ahmad
Published: May 2021

Calprotectin (CP) is a non-covalent heterodimer formed by the subunits S100A8 (A8) and S100A9 (A9). When neutrophils become activated, undergo disruption, or die, this abundant cytosolic neutrophil protein is released. By fervently chelating trace metal ions that are essential for bacterial development, CP plays an important role in human innate immunity. It also serves as an alarmin by controlling the inflammatory response after it is released. Extracellular concentrations of CP increase in response to infection and inflammation, and are used as a biomarker of neutrophil activation in a variety of inflammatory diseases. Although it has been almost 40 years since CP was discovered, its use in daily pediatric practice is still limited. Current evidence suggests that CP could be used as a biomarker in a variety of pediatric respiratory diseases, and could become a valuable key factor in promoting diagnostic and therapeutic capacity. The aim of this study is to re-introduce CP to the medical community and to emphasize its potential role with the hope of integrating it as a useful adjunct, in the practice of pediatric respiratory medicine.
Diagnostic Accuracy of 3D Ultrasound and Artificial Intelligence for Detection of Pediatric Wrist Injuries
Authors: Zhang Jack , Boora Naveenjyote , Melendez Sarah , Rakkunedeth Hareendranathan Abhilash , Jaremko Jacob
Published: May 2021

Wrist trauma is common in children, typically requiring radiography for diagnosis and treatment planning. However, many children do not have fractures and are unnecessarily exposed to radiation. Ultrasound performed at bedside could detect fractures prior to radiography. Modern tools including three-dimensional ultrasound (3DUS) and artificial intelligence (AI) have not yet been applied to this task. Our purpose was to assess (1) feasibility, reliability, and accuracy of 3DUS for detection of pediatric wrist fractures, and (2) accuracy of automated fracture detection via AI from 3DUS sweeps. Children presenting to an emergency department with unilateral upper extremity injury to the wrist region were scanned on both the affected and unaffected limb. Radiographs of the symptomatic limb were obtained for comparison. Ultrasound scans were read by three individuals to determine reliability. An AI network was trained and compared against the human readers. Thirty participants were enrolled, resulting in scans from fifty-five wrists. Readers had a combined sensitivity of 1.00 and specificity of 0.90 for fractures. AI interpretation was indistinguishable from human interpretation, with all fractures detected in the test set of 36 images (sensitivity = 1.0). The high sensitivity of 3D ultrasound and automated AI ultrasound interpretation suggests that ultrasound could potentially rule out fractures in the emergency department.
Combined Single Nucleotide Variants of ORAI1 and BLK in a Child with Refractory Kawasaki Disease
Authors: Kanda Saki , Fujii Yoshimitsu , Hori Shin-ichiro , Ohmachi Taichi , Yoshimura Ken , Higasa Koichiro , Kaneko Kazunari
Published: May 2021

Kawasaki disease (KD) is a systemic vasculitis with an unknown etiology affecting young children. Although intravenous immunoglobulin (IVIG) plus acetylsalicylic acid is effective in most cases, approximately 10–20% of patients do not respond to this therapy. An 8-month-old boy was admitted to a local hospital with the presumptive diagnosis of KD. He received IVIG twice and four series of methylprednisolone pulse therapy from the third to the tenth day of illness. Despite these treatments, his fever persisted with the development of moderate dilatations of the coronary arteries. A diagnosis of refractory KD was made, and infliximab with oral prednisolone was administered without success. Defervescence was finally achieved by cyclosporine A, an inhibitor of the signaling pathway of the calcineurin/nuclear factor of activated T cells (NFAT). Whole-genome sequencing of his deoxyribonucleic acid samples disclosed two single nucleotide variants (SNVs) in disease-susceptibility genes in Japanese KD patients, ORAI1 (rs3741596) and BLK (rs2254546). In summary, the refractory nature of the present case could be explained by the presence of combined SNVs in susceptibility genes associated with upregulation of the calcineurin/NFAT signaling pathway. It may provide insights for stratifying KD patients based on the SNVs in their susceptibility genes.
Complications and Recurrence of Patellar Instability after Medial Patellofemoral Ligament Reconstruction in Children and Adolescents: A Systematic Review
Authors: D’Ambrosi Riccardo , Corona Katia , Capitani Paolo , Coccioli Gianluca , Ursino Nicola , Peretti Giuseppe Maria
Published: May 2021

Background: This study aimed to review the data available in the current literature concerning the complications and recurrence of instability following medial patellofemoral ligament (MPFL) reconstruction for patellar instability in young and adolescent patients (those <20 years old). Methods: A systematic review was performed based on the preferred reporting items for systematic reviews and meta-analyses (PRISMA) guidelines. Two independent reviewers searched the PubMed, Scopus, EMBASE, and Cochrane databases. The terms “medial patellofemoral ligament” or “MPFL” and “reconstruction” and “young” or “adolescents” or “children” were used. The inclusion criteria for the literature review comprised studies that reported the complications and recurrences of instability in patients who had undergone MPFL reconstruction for patellar instability. Results: In all, 332 patients were included in the review, of which 195 were females (63.5%) and 112 were males (36.5%), and they totaled 352 treated knees. The mean age at the time of the surgery was 14.28 years, and the mean follow-up duration was 30.17 months. A total of 16 (4.5%) complications were reported: one (0.3%) patella fracture, one (0.3%) screw removal due to intolerance, one (0.3%) infection, five (1.4%) wound complications, six (1.7%) subluxations and two (0.6%) instances of post-operative stiffness. A total of 18 (5.1%) recurrences of patellar instability were recorded. Conclusions: MPFL reconstruction in young patients can be considered an effective and safe treatment leading to clinical improvement in terms of recurrence of dislocation. No major complications related to the technique were reported, but a high level of research evidence is required to better evaluate the clinical results in a long-term follow-up.
Improving the Shelf Life of Peeled Fresh Almond Kernels by Edible Coating with Mastic Gum
Authors: Farooq Muhammad , Azadfar Elham , Rusu Alexandru , Trif Monica , Poushi Mahmoud Kohneh , Wang Yunyang
Published: May 2021

Coating, as a process in which fruits, vegetables, kernels, and nuts are covered with an edible layer, is an environmentally friendly alternative to plastic wrapping, which has been considered the most effective way to preserve them over the long term. On the other hand, prolonging the shelf life results in a reduction of spoilage and therefore achieving a goal that is very important nowadays—the reduction of food waste. The quality of preserved almonds kernels depends on factors such as grain moisture, storage temperature, relative humidity, oxygen level, packaging, and the shape of the stored nuts (along with being peeled, unpeeled, roasted, etc.). The commercial importance of the almond fruit is related to its kernel. Almonds that are peeled (without the thin brown skin) and stored have a shorter shelf life than unpeeled almonds since the reddish-brown skin, rich in antioxidants, may protect the kernels against oxidation. In this study, a bioactive edible coating has been tested, which may provide an effective barrier against oxygen permeation and moisture, thus preserving the quality of peeled fresh almonds by extending their shelf life. Mastic gum, as a natural coating agent, was used to coat the peeled fresh almond kernels in four different concentrations (0.5%, 1.0%, 1.5%, and 2.0% w/v). The effect of mastic gum coating on the quality parameters of the peeled fresh almonds (moisture uptake, oil oxidation, total yeast and mold growth, and Aspergillus species development) was studied during four months of storage. The results showed that mastic gum, as a coating agent, significantly (p < 0.05) reduced moisture absorption, peroxide and thiobarbituric acid indices, total yeast and mold growth, and Aspergillus species development in the peeled and coated fresh almonds, compared to the control, i.e., uncoated fresh almonds, during 4 months of storage, packed at room temperature (25–27 °C) inside a cabinet at 90% humidity. Therefore, mastic gum can be used as a great natural preservative coating candidate with antioxidant and antimicrobial effects.
Erratum: Zafar et al. Synthesis and Characterization of Potent and Safe Ciprofloxacin-Loaded Ag/TiO2/CS Nanohybrid against Mastitis-Causing E. coli. Crystals 2021, 11, 319
Authors: Zafar Naheed , Uzair Bushra , Niazi Muhammad Bilal Khan , Samin Ghufrana , Bano Asma , Jamil Nazia , Waqar-Un-Nisa  , Sajjad Shamaila , Menaa Farid
Published: May 2021

We sincerely apologize for the inconvenience of updating the authorship [...]
Mature and Immature/Activated Cells Fractionation: Time for a Paradigm Shift in Differential Leucocyte Count Reporting?
Authors: Haider Rana Zeeshan , Khan Najeed Ahmed , Urrechaga Eloisa , Shamsi Tahir Sultan
Published: May 2021

Leucocytes, especially neutrophils featuring pro- and anti-cancerous characteristics, are involved in nearly every stage of tumorigenesis. Phenotypic and functional differences among mature and immature neutrophil fractions are well reported, and their correlation with tumor progression and therapy has emerging implications in modern oncology practices. Technological advancements enabled modern hematology analyzers to generate extended information (research parameters) during complete blood cell count (CBC) analysis. We hypothesized that neutrophil and lymphocyte fractions-related extended differential leucocytes count (DLC) parameters hold superior diagnostic utility over routine modalities. The present study was carried out over a four-and-a-half-year period wherein extended neutrophil (immature granulocyte [IG] and mature neutrophil [NEUT#&]), and lymphocyte (activated/high fluorescence lymphocyte count [HFLC] and resting lymphocyte [LYMP#&]) parameters were challenged over routine neutrophil [NEUT#] and lymphocyte [LYMP#] items in a study population of 1067 hematological neoplasm patients. Extending the classical statistical approaches, machine-learning-backed data visualization was used to explore trends in the study parameters. As a whole, extended neutrophil and lymphocyte count outperformed and was diagnostically more relevant than routine neutrophil and lymphocyte parameters by showing the least difference from their respective (gold-standard) manual DLC counts. The mature neutrophil count was compared to IG, and resting lymphocyte count was compared to HFLC by calling the function ‘correlation’ as a ‘clustering function’ for heatmap based visualization. The aforementioned study parameters displayed close clustering (rearrangement) for their respective study items by presenting distinct trends of equally valuable weights (deviated values), advocating fractions-based extended DLC reporting. Importantly, using a Bland and Altman analysis analogously to a manual neutrophil count, the mature neutrophil count [NEUT#&] remained unbiased since a routine neutrophil count [NEUT#] was found to be a negatively biased. The extended DLC-parameter-driven fractions-based reporting has superior diagnostic utility over classical routine approaches; this finding can largely minimize labor-intensive manual DLC practices, especially in hematology–oncology departments.
An AI-Enabled Stock Prediction Platform Combining News and Social Sensing with Financial Statements
Authors: Theodorou Traianos-Ioannis , Zamichos Alexandros , Skoumperdis Michalis , Kougioumtzidou Anna , Tsolaki Kalliopi , Papadopoulos Dimitris , Patsios Thanasis , Papanikolaou George , Konstantinidis Athanasios , Drosou Anastasios , Tzovaras Dimitrios
Published: May 2021

In recent years, the area of financial forecasting has attracted high interest due to the emergence of huge data volumes (big data) and the advent of more powerful modeling techniques such as deep learning. To generate the financial forecasts, systems are developed that combine methods from various scientific fields, such as information retrieval, natural language processing and deep learning. In this paper, we present ASPENDYS, a supportive platform for investors that combines various methods from the aforementioned scientific fields aiming to facilitate the management and the decision making of investment actions through personalized recommendations. To accomplish that, the system takes into account both financial data and textual data from news websites and the social networks Twitter and Stocktwits. The financial data are processed using methods of technical analysis and machine learning, while the textual data are analyzed regarding their reliability and then their sentiments towards an investment. As an outcome, investment signals are generated based on the financial data analysis and the sensing of the general sentiment towards a certain investment and are finally recommended to the investors.
AI-Based Analysis of Policies and Images for Privacy-Conscious Content Sharing
Authors: Contu Francesco , Demontis Andrea , Dessì Stefano , Muscas Marco , Riboni Daniele
Published: May 2021

Thanks to the popularity of personal mobile devices, more and more of the different types of private content, such as images and videos, are shared on social networking applications. While content sharing may be an effective practice to enhance social relationships, it is also a source of relevant privacy issues. Unfortunately, users find it difficult to understanding the terms and implications of the privacy policies of apps and services. Moreover, taking privacy decisions about content sharing on social networks is cumbersome and prone to errors that could determine privacy leaks. In this paper, we propose two techniques aimed at supporting the user in taking privacy choices about sharing personal content online. Our techniques are based on machine learning and natural language processing to analyze privacy policies, and on computer vision to assist the user in the privacy-conscious sharing of multimedia content. Experiments with real-world data show the potential of our solutions. We also present ongoing work on a system prototype and chatbot for natural language user assistance.
Extrusion Processing of Rapeseed Press Cake-Starch Blends: Effect of Starch Type and Treatment Temperature on Protein, Fiber and Starch Solubility
Authors: Martin Anna , Naumann Susanne , Osen Raffael , Karbstein Heike Petra , Emin M. Azad
Published: May 2021

For the valorization of oilseed press cakes into food products, extrusion can be used. A common way of applying the protein- and fiber-rich press cakes in directly expanded products is the combination thereof with starch, since starch gives a favourable texture, which correlates directly to expansion. To control product properties like expansion of protein and fiber-rich extruded products, the underlying physicochemical changes of proteins, fibers and starch due to thermomechanical input need to be comprehensively described. In this study, rapeseed press cake (RPC) was extruded and treated under defined thermomechanical conditions in a closed-cavity rheometer, pure and in combination with four starches. The impact of starch type (potato PS, waxy potato WPS, maize MS, high-amylose maize HAMS) and temperature (20/25, 80, 100, 120, 140 °C) on protein solubility, starch gelatinization (Dgel), starch hydrolysis (SH) and fiber solubility of the blends was evaluated. The extrusion process conditions were significantly affected by the starch type. In the extruded blends, the starch type had a significant impact on the protein solubility which decreased with increasing barrel temperature. Increasing barrel temperatures significantly increased the amount of soluble fiber fractions in the blends. At defined thermomechanical conditions, the starch type showed no significant impact on the protein solubility of the blends. Therefore, the observed effects of starch type on the protein solubility of extruded blends could be attributed to the indistinct process conditions due to differences in the rheological properties of the starches rather than to molecular interactions of the starches with the rapeseed proteins in the blends.