bluuebunny/crossref_metadata_2025 huggingface dataset:
PERFIL DE UTILIZAÇÃO DE PLANTAS MEDICINAIS POR MORADORES DA ZONA RURAL DO MUNICÍPIO DE SÃO GONÇALO DO SAPUCAÍ–MG
Authors: Dos Reis Ana Carolina Alves , Mudrik Paula
Published: May 2019

O consumo de plantas medicinais é uma prática passada de geração em geração que se estende por longos anos. O presente trabalho teve como objetivo analisar o perfil de utilização de plantas medicinais por moradores da zona rural (distritos) do município de São Gonçalo do Sapucaí – MG, determinando as plantas medicinais mais utilizadas e a forma de utilização, para quais tipos de doenças são utilizadas e o conhecimento do consumidor sobre os seusefeitos colaterais. Foi usada a metodologia de questionários semiestruturados. O estudo foi realizado em quatro distritos (Água Comprida, Carneiros, Ferreiras e Ribeiro) da cidade, sendo aplicados 100 questionários, resultando em 48 plantas citadas como medicinais pelos entrevistados. As principais finalidades de uso foram calmantes (85 citações) e contra resfriado (70 citações). A maioria das plantas medicinais utilizadas são preparadas na forma de chás (88 citações) e obtidas por plantação própria (92 citações). Quanto ao conhecimento sobre os efeitos colaterais, apenas 11,34% da população estudada conhece esses efeitos, o que se torna uma situação muito preocupante. No entanto, 95,88% dos entrevistados nunca tiveram problemas depois de utilizar alguma planta. Apesar do fato da incidência de problemas ter sido pequena, algumas plantas possuem efeitos colaterais comprovados cientificamente.
Archaeoacoustic Examination of Lazarica Church
Authors: Đorđević Zorana , Novković Dragan , Andrić Uroš
Published: May 2019

The acoustic analysis provides additional information on building tradition and related indoor practice that includes sound, thus deepening our understanding of architectural heritage. In this paper, the sound field of the Orthodox medieval church Lazarica (Kruševac city, Serbia) is examined. Lazarica is a representative of Morava architectural style, developed in the final period of the Serbian medieval state, when also the chanting art thrived, proving the importance of the aural environment in Serbian churches. The church plan is a combination of a traditional inscribed cross and a triconch. After the in situ measurement of acoustic impulse response using EASERA software, we built a computer model in the acoustic simulation software EASE and calibrated it accordingly. Following the parameters (reverberation time (T30), early decay time (EDT) and speech transmission index (STI)), we examined the acoustic effect of the space occupancy, central dome and the iconostasis. In all the cases, no significant deviation between T30 and EDT parameter was observed, which indicates uniform sound energy decay. Closing the dome with a flat ceiling did not show any significant impact on T30, but it lowered speech intelligibility. The height of iconostasis showed no significant influence on the acoustics of Lazarica church.
Using Social Media to Identify Consumers’ Sentiments towards Attributes of Health Insurance during Enrollment Season
Authors: van den Broek-Altenburg Eline M. , Atherly Adam J.
Published: May 2019

This study aims to identify sentiments that consumers have about health insurance by analyzing what they discuss on Twitter. The objective was to use sentiment analysis to identify attitudes consumers express towards health insurance and health care providers. We used an Application Programming Interface to gather tweets from Twitter with the words “health insurance” or “health plan” during health insurance enrollment season in the United States in 2016‒2017. Word association was used to find words associated with “premium,” “access,” “network,” and “switch.” Sentiment analysis established which specific emotions were associated with insurance and medical providers, using the NRC Emotion Lexicon, identifying emotions. We identified that provider networks, prescription drug benefits, political preferences, and norms of other consumers matter. Consumers trust medical providers but they fear unexpected health events. The results suggest that there is a need for different algorithms to help consumers find the plans they want and need. Consumers buying health insurance in the Affordable Care Act marketplaces in the United States choose lower-cost plans with limited benefits, but at the same time express fear about unexpected health events and unanticipated costs. If we better understand the origin of the sentiments that drive consumers, we may be able to help them better navigate insurance plan options and insurers can better respond to their needs.
Corpus Augmentation for Neural Machine Translation with Chinese-Japanese Parallel Corpora
Authors: Zhang Jinyi , Matsumoto Tadahiro
Published: May 2019

The translation quality of Neural Machine Translation (NMT) systems depends strongly on the training data size. Sufficient amounts of parallel data are, however, not available for many language pairs. This paper presents a corpus augmentation method, which has two variations: one is for all language pairs, and the other is for the Chinese-Japanese language pair. The method uses both source and target sentences of the existing parallel corpus and generates multiple pseudo-parallel sentence pairs from a long parallel sentence pair containing punctuation marks as follows: (1) split the sentence pair into parallel partial sentences; (2) back-translate the target partial sentences; and (3) replace each partial sentence in the source sentence with the back-translated target partial sentence to generate pseudo-source sentences. The word alignment information, which is used to determine the split points, is modified with “shared Chinese character rates” in segments of the sentence pairs. The experiment results of the Japanese-Chinese and Chinese-Japanese translation with ASPEC-JC (Asian Scientific Paper Excerpt Corpus, Japanese-Chinese) show that the method substantially improves translation performance. We also supply the code (see Supplementary Materials) that can reproduce our proposed method.
Comparing Fish Density and Echo Strength Distribution Recorded by Two Generations of Single Beam Echo Sounders
Authors: Linløkken Arne N. , Næstad Frode , Langdal Kjell , Østbye Kjartan
Published: May 2019

Hydroacoustic acquisition was performed by means of two different single beam systems, the Simradmodel EK15 from 2015 and the Simrad model EY-M from the 1980s to explore potential differences of fish density and target strength (TS) distribution between EK15 and EY-M-based estimates. The oligotrophic Lake Storsjøen (48 km2) with steep shores, was chosen for the survey. The pelagic fish stock is dominated by whitefish (Coregonus lavaretus L.), and, recently, illegally stocked smelt (Osmerus eperlanus L.), and a low proportion of Arctic charr (Salvelinus alpinus L.). The total density was estimated at two depth layers (18–32 and 32–48 m), and was quite similar for TS ≥ −56 dB: 755 and 498 fish ha−1, respectively for the EK15-based estimates and similarly 766 and 490 fish ha−1 for the EY-M estimate. Target strength distributions were similar for TS > −48 dB. The proportion of single fish detected with EK15 was negatively affected by the long pulse duration. Six acquisitions from 1986 to 2016 showed a dramatic increase of density of TS = −46 to −44 dB echoes (>10 cm) between 2013 and 2016. This was due to the growth of the introduced smelt population.
FMnet: Iris Segmentation and Recognition by Using Fully and Multi-Scale CNN for Biometric Security
Authors: Tobji Rachida , Di Wu , Ayoub Naeem
Published: May 2019

In Deep Learning, recent works show that neural networks have a high potential in the field of biometric security. The advantage of using this type of architecture, in addition to being robust, is that the network learns the characteristic vectors by creating intelligent filters in an automatic way, grace to the layers of convolution. In this paper, we propose an algorithm “FMnet” for iris recognition by using Fully Convolutional Network (FCN) and Multi-scale Convolutional Neural Network (MCNN). By taking into considerations the property of Convolutional Neural Networks to learn and work at different resolutions, our proposed iris recognition method overcomes the existing issues in the classical methods which only use handcrafted features extraction, by performing features extraction and classification together. Our proposed algorithm shows better classification results as compared to the other state-of-the-art iris recognition approaches.
Role of the Hippo Pathway in Fibrosis and Cancer
Authors: Kim Cho-Long , Choi Sue-Hee , Mo Jung-Soon
Published: May 2019

The Hippo pathway is the key player in various signaling processes, including organ development and maintenance of tissue homeostasis. This pathway comprises a core kinases module and transcriptional activation module, representing a highly conserved mechanism from Drosophila to vertebrates. The central MST1/2-LATS1/2 kinase cascade in this pathway negatively regulates YAP/TAZ transcription co-activators in a phosphorylation-dependent manner. Nuclear YAP/TAZ bind to transcription factors to stimulate gene expression, contributing to the regenerative potential and regulation of cell growth and death. Recent studies have also highlighted the potential role of Hippo pathway dysfunctions in the pathology of several diseases. Here, we review the functional characteristics of the Hippo pathway in organ fibrosis and tumorigenesis, and discuss its potential as new therapeutic targets.
pHluorin-BACE1-mCherry Acts as a Reporter for the Intracellular Distribution of Active BACE1 In Vitro and In Vivo
Authors: Zhao Lu , Zhao Yang , Tang Fu-Lei , Xiong Lei , Su Ce , Mei Lin , Zhu Xiao-Juan , Xiong Wen-Cheng
Published: May 2019

β-site APP-cleaving enzyme 1 (BACE1) initiates amyloid precursor protein (APP) cleavage and β-amyloid (Aβ) production, a critical step in the pathogenesis of Alzheimer’s disease (AD). It is thus of considerable interest to investigate how BACE1 activity is regulated. BACE1 has its maximal activity at acidic pH and GFP variant—pHluorin—displays pH dependence. In light of these observations, we generated three tandem fluorescence-tagged BACE1 fusion proteins, named pHluorin-BACE1-mCherry, BACE1-mCherry-pHluorin and BACE1-mCherry-EGFP. Comparing the fluorescence characteristics of these proteins in response to intracellular pH changes induced by chloroquine or bafilomycin A1, we found that pHluorin-BACE1-mCherry is a better pH sensor for BACE1 because its fluorescence intensity responds to pH changes more dramatically and more quickly. Additionally, we found that (pro)renin receptor (PRR), a subunit of the v-ATPase complex, which is critical for maintaining vesicular pH, regulates pHluorin’s fluorescence and BACE1 activity in pHluorin-BACE1-mCherry expressing cells. Finally, we found that the expression of Swedish mutant APP (APPswe) suppresses pHluorin fluorescence in pHluorin-BACE1-mCherry expressing cells in culture and in vivo, implicating APPswe not only as a substrate but also as an activator of BACE1. Taken together, these results suggest that the pHluorin-BACE1-mCherry fusion protein may serve as a useful tool for visualizing active/inactive BACE1 in culture and in vivo.
The Effect of Shift Work and Poor Sleep on Self-Reported Skin Conditions: A Survey of Call Center Agents in the Philippines
Authors: Lu Francine , Suggs Amanda , Ezaldein Harib , Ya Jason , Fu Pingfu , Jamora Jasmin , Verallo-Rowel Vermen , Baron Elma
Published: May 2019

Night shift workers may have a disrupted circadian rhythm, which may contribute to the development of skin disease. The purpose of this study was to determine whether there is a significant difference in the prevalence and severity of self-reported skin disease between “regular” day shift workers compared to “graveyard” night shift workers. We conducted surveys from 630 call center agents in Manila, the Philippines, and they were analyzed regarding demographics, medical history, dermatologic history, lifestyle, and sleep. No difference was found in the prevalence of skin disease between shifts. However, night shift workers were worse sleepers. When compared to good sleepers, poor sleepers had a higher prevalence of skin disease with worse severity. Graveyard shift workers with poor sleep may have increased skin disease severity.
Protein Crystallization in Ionic-Liquid Hydrogel Composite Membranes
Authors: Belviso Benny Danilo , Caliandro Rosanna , Salehi Shabnam Majidi , Di Profio Gianluca , Caliandro Rocco
Published: May 2019

Protein crystallization is a powerful purification tool. It is the first step for crystallographic structural investigations, and can be preparatory for biotechnological applications. However, crystallizing proteins is challenging and methods to control the crystallization process are needed. Ionic-liquid hydrogel composite membranes (IL-HCMs) have been used here as material capable of supporting protein crystallization and hosting grown crystals. We found that IL-HCMs affect the selection mechanism of glucose isomerase (GI) polymorphs and make GI crystals grow completely immersed into the hydrogel layer. X-ray diffraction studies show that IL ions do not bind to the protein, likely because IL molecules are constrained in the polymeric framework. Our GI crystal structures have been compared with many existing GI crystal structures using multivariate analysis tools, allowing a comprehensive overview of factors determining structural similarities, i.e., temperature variations and external stresses exerted during or after crystal growth, such as dehydration or presence of hydrogel of a different nature. GI crystals grown on IL-HCM fit perfectly in this framework, showing typical features induced by external forces. Overall, protein crystallization by IL-HCMs show potential for biotechnological applications, as it could constitute a natural means for containing crystallized enzymes in working conditions.
High Risk of Post-Earthquake Fire Hazard in Dhaka, Bangladesh
Authors: Mondal Dhiman R.
Published: May 2019

According to a recent survey conducted by the Fire Service and Civil Defense in Dhaka, Bangladesh, more than 400 hospitals and clinics are facing a dreadful risk of fire hazard [...]
Identification and Validation of Novel Reference Genes in Acute Lymphoblastic Leukemia for Droplet Digital PCR
Authors: Villegas-Ruíz Vanessa , Olmos-Valdez Karina , Castro-López Kattia Alejandra , Saucedo-Tepanecatl Victoria Estefanía , Ramírez-Chiquito Josselen Carina , Pérez-López Eleazar Israel , Medina-Vera Isabel , Juárez-Méndez Sergio
Published: May 2019

Droplet digital PCR is the most robust method for absolute nucleic acid quantification. However, RNA is a very versatile molecule and its abundance is tissue-dependent. RNA quantification is dependent on a reference control to estimate the abundance. Additionally, in cancer, many cellular processes are deregulated which consequently affects the gene expression profiles. In this work, we performed microarray data mining of different childhood cancers and healthy controls. We selected four genes that showed no gene expression variations (PSMB6, PGGT1B, UBQLN2 and UQCR2) and four classical reference genes (ACTB, GAPDH, RPL4 and RPS18). Gene expression was validated in 40 acute lymphoblastic leukemia samples by means of droplet digital PCR. We observed that PSMB6, PGGT1B, UBQLN2 and UQCR2 were expressed ~100 times less than ACTB, GAPDH, RPL4 and RPS18. However, we observed excellent correlations among the new reference genes (p < 0.0001). We propose that PSMB6, PGGT1B, UBQLN2 and UQCR2 are housekeeping genes with low expression in childhood cancer.
Site Effect Assessment in Ulaanbaatar, Mongolia through Inversion Analysis of Microtremor H/V Spectral Ratios
Authors: Tumurbaatar Zorigt , Miura Hiroyuki , Tsamba Tsoggerel
Published: May 2019

Due to the population growth and urban sprawl in Ulaanbaatar city (UB), Mongolia, hazard and risk analysis for future earthquakes have become an important issue for disaster mitigation planning. Evaluation of a site effect is one of the essential parts of the earthquake hazard estimation in this area. The site effect can be evaluated by site amplifications calculated from shear-wave velocity (VS) models including from bedrock to surface layers. However, it is difficult to assess the pattern of the site effects in UB because shallow mostly up to 15 m and a small number of investigated VS models are available in previous studies. In this study, the VS models are estimated using microtremor data at 50 sites and inversion analysis is applied to the observed data in order to evaluate site amplifications in UB. In particular, the joint inversion technique based on a diffuse field approach is applied to estimate the VS structures at three sites using the observed horizontal-to-vertical (H/V) spectral ratios and surface wave phase velocities obtained by Odonbaatar (2011). The rest of the sites are estimated by the single inversion technique using the observed microtremor H/V spectral ratios considering the results of the joint inversions. The seismic microzoning in UB is performed based on the site amplifications computed from the inverted VS models to characterize the pattern of seismic hazard. The result shows the largest site amplification zone is computed along the Tuul river in the southeastern part of UB.
Rubella Vaccination Coverage Among Women of Childbearing Age in Vietnam
Authors: Do Toan Thanh Thi , Nguyen Anh Ngoc , Le Xuan Thanh Thi , Pongsakul Ann , Nguyen Quang Nhat , Nguyen Thanh Van , Nguyen Thang Huu , Do Tri Minh , Le Huong Thi , Nguyen Huong Lan Thi , Truong Nu Thi , Hoang Chi Linh , Vu Giang Thu , Tran Tung Thanh , Tran Tung Hoang , Tran Bach Xuan , Latkin Carl A. , Ho Cyrus SH , Ho Roger CM
Published: May 2019

Despite the availability of effective and safe rubella vaccines for women of childbearing age, prevention and control of congenital rubella syndrome in children remains challenging in Vietnam. In order to examine this issue, we conducted a cross-sectional study, examining the current coverage of rubella vaccination before pregnancy among 807 pregnant women and women with children under 12 months of age in urban and rural districts, Dong Da and Ba Vi, in Hanoi, Vietnam. In this population, we observed an alarming non-compliance rate with rubella vaccination before pregnancy in both localities. Among the 82.0% of participants who remained unvaccinated against this contagious viral infection, 95.8% of them were in Ba Vi district, compared to 68.0% in Dong Da district (p < 0.001). Besides the differences in age, number of children, education levels, primary occupations and monthly incomes among the participants between the two districts, other reasons for noncompliance with rubella vaccination includeddisinterest in rubella vaccination, the high cost and long distance to vaccination sites as well as unawareness of vaccination locations. In addition to addressing the unique socio-economicchallenges behind one’s accessibility to vaccination services in urban and rural areas, our study supports a continued effort in ensuring proper access to and education about pre-pregnancy vaccines and vaccination among women of childbearing age in order to achieve and sustain sufficient immunization coverage of rubella and other vaccine-preventable diseases in both settings.
Eight Weeks of Inspiratory Muscle Training Improves Pulmonary Function in Disabled Swimmers—A Randomized Trial
Authors: Okrzymowska Paulina , Kurzaj Monika , Seidel Wojciech , Rożek-Piechura Krystyna
Published: May 2019

Background: According to the literature, inspiratory muscle fatigue may increase after swimming training (ST). This study aimed to examine the efficacy of 8-week inspiratory muscular training (IMT) in disabled swimmers, combined with standard sports training, on selected parameters of lung ventilation and the function of respiratory muscles. Methods: A total of 16 disabled swimming division athletes from Wroclaw’s ‘Start’ Regional Sports Association qualified for the study. The subjects were randomly divided into two groups (ST and IMT). Both groups participated in swimming training for 8 weeks (8 times a week). The IMT group additionally participated in inspiratory muscle training (8 weeks). In all respondents, a functional lung test and the respiratory muscle strength was measured. Results: After 8 weeks of training, a significant increase in ventilation parameters and respiratory muscle strength was observed only in the IMT group. In ST group 1, a 20% improvement in the strength of inspiratory muscles was achieved. Conclusions: The inclusion of IMT is an important element that complements swimming training, allowing for greater increases in lung ventilation parameters and the strength of respiratory muscles in disabled swimmers.
Risk Factors and Prevalence of Abdominal Obesity among Upper-Secondary Students
Authors: Błaszczyk-Bębenek Ewa , Piórecka Beata , Płonka Małgorzata , Chmiel Izabela , Jagielski Paweł , Tuleja Katarzyna , Schlegel-Zawadzka Małgorzata
Published: May 2019

Inadequate eating habits, as well as a low level of physical activity, influence adipose tissue deposition. The aim of the study was to assess the prevalence of central obesity in upper-secondary students and to determine the factors related to its occurrence. The survey included 309 participants, aged 16 to 18 years from Krakow (Poland). Anthropometric measurements were taken during the periodic assessment of students’ health status. An anonymous questionnaire was used to assess the nutritional and non-nutritional risk factors of participants. According to different methods of measurement, abdominal obesity (AO) was observed in 15.5% (WC—waist circumference), 10.7% (WHtR—waist to height ratio) or 21.7% (WHR—waist to hip ratio) participants. Abdominal obesity (WC) was significantly associated with family history of excess body weight and higher economic status of the family. The risk of AO (WC) was significantly lower among adolescents who declared higher physical activity. Boys who eat first breakfasts have lower AO risk according to WHtR interpretation. Abdominal obesity in gender group was related to the self-esteem of one’s own appearance according to WHtR and WC. Abdominal obesity was associated with the family environment and modifiable lifestyle factors and was dependent on gender.
Response of Soil Microbes to Vegetation Restoration in Coal Mining Subsidence Areas at Huaibei Coal Mine, China
Authors: Sun Shiyong , Sun Hui , Zhang Deshun , Zhang Jianfeng , Cai Zeyu , Qin Guanghua , Song Yumin
Published: May 2019

Vegetation restoration is an available way to ameliorate degraded lands. In order to study the response of soil microbes to vegetation restoration in coal mining subsidence areas, the composition and distribution of soil microbes were discussed through three plots: unsubsided area (CA), new subsided area (NSA), and old subsided area (OSA) with different vegetation restoration time in Huabei coal mine. Meanwhile, changes in soil catalase and urease activity were explored and the correlation between soil bacteria, fungi, and environmental factors was analysed. The results demonstrated that Nitrospira was the dominant bacteria in all areas sampled. Microorganisms in the 0–20 cm and 40–60 cm soil layers of OSA had the highest Simpson index, whereas the index in NSA was lowest (at all soil depths). The catalase activity in NSA was significantly higher than that in CA, and there was no significant difference in catalase activity with soil depth, while the urease activity declined gradually with increasing soil depth. The urease activity in the 20–60 cm soil layer of NSA and OSA was significantly higher than that of CA. Furthermore, the distribution of bacteria was mainly affected by soil organic matter, available potassium, available phosphorus, and alkali-hydrolyzable nitrogen, whereas pH and catalase activity mainly affected fungal distribution. These results implied that soil catalase activity in NSA and urease activity in the 20–40 cm soil layer of NSA and OSA were significantly enhanced after vegetation restoration, and that long-term plant restoration could improve soil fertility and soil microbial community diversity in coal mining areas.
Impacts of Seasonal and Annual Weather Variations on Network-Level Pavement Performance
Authors: Gao Lu , Hong Feng , Ren Yi-Hao (Wilson)
Published: May 2019

This study was aimed to determine the extent of association between network-level pavement condition and seasonal and annual weather variations. Pavement condition data recorded in the Texas Department of Transportation’s Pavement Management Information System (PMIS) database between 2000 and 2008 was highlighted. Meteorological data collected at different districts across the whole state were used in the explanatory variables. Dynamic panel data analysis was used in the deterioration models to quantify the effects of temperature and precipitation variations on pavement conditions. Based on the statistical implication from the model estimation results, significant correlations were identified between pavement conditions, the average monthly rainfall, and the average monthly temperature recorded 1 to 23 months prior to pavement condition inspection.
Phosphoproteomic Profiling Identifies Aberrant Activation of Integrin Signaling in Aggressive Non-Type Bladder Carcinoma
Authors: Deb Barnali , Puttamallesh Vinuth N. , Gondkar Kirti , Thiery Jean P. , Gowda Harsha , Kumar Prashant
Published: May 2019

Bladder carcinoma is highly heterogeneous and its complex molecular landscape; thus, poses a significant challenge for resolving an effective treatment in metastatic tumors. We computed the epithelial-mesenchymal transition (EMT) scores of three bladder carcinoma subtypes—luminal, basal, and non-type. The EMT score of the non-type indicated a “mesenchymal-like” phenotype, which correlates with a relatively more aggressive form of carcinoma, typified by an increased migration and invasion. To identify the altered signaling pathways potentially regulating this EMT phenotype in bladder cancer cell lines, we utilized liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based phosphoproteomic approach. Bioinformatics analyses were carried out to determine the activated pathways, networks, and functions in bladder carcinoma cell lines. A total of 3125 proteins were identified, with 289 signature proteins noted to be differentially phosphorylated (p ≤ 0.05) in the non-type cell lines. The integrin pathway was significantly enriched and five major proteins (TLN1, CTTN, CRKL, ZYX and BCAR3) regulating cell motility and invasion were hyperphosphorylated. Our study reveals GSK3A/B and CDK1 as promising druggable targets for the non-type molecular subtype, which could improve the treatment outcomes for aggressive bladder carcinoma.
Optimization of MBE Growth Conditions of In0.52Al0.48As Waveguide Layers for InGaAs/InAlAs/InP Quantum Cascade Lasers
Authors: Gutowski Piotr , Sankowska Iwona , Słupiński Tomasz , Pierścińska Dorota , Pierściński Kamil , Kuźmicz Aleksandr , Gołaszewska-Malec Krystyna , Bugajski Maciej
Published: May 2019

We investigate molecular beam epitaxy (MBE) growth conditions of micrometers-thick In0.52Al0.48As designed for waveguide of InGaAs/InAlAs/InP quantum cascade lasers. The effects of growth temperature and V/III ratio on the surface morphology and defect structure were studied. The growth conditions which were developed for the growth of cascaded In0.53Ga0.47As/In0.52Al0.48As active region, e.g., growth temperature of Tg = 520 °C and V/III ratio of 12, turned out to be not optimum for the growth of thick In0.52Al0.48As waveguide layers. It has been observed that, after exceeding ~1 µm thickness, the quality of In0.52Al0.48As layers deteriorates. The in-situ optical reflectometry showed increasing surface roughness caused by defect forming, which was further confirmed by high resolution X-ray reciprocal space mapping, optical microscopy and atomic force microscopy. The presented optimization of growth conditions of In0.52Al0.48As waveguide layer led to the growth of defect free material, with good optical quality. This has been achieved by decreasing the growth temperature to Tg = 480 °C with appropriate increasing V/III ratio. At the same time, the growth conditions of the cascade active region of the laser were left unchanged. The lasers grown using new recipes have shown lower threshold currents and improved slope efficiency. We relate this performance improvement to reduction of the electron scattering on the interface roughness and decreased waveguide absorption losses.
Fabrication and Characterization of Low Methoxyl Pectin/Gelatin/Carboxymethyl Cellulose Absorbent Hydrogel Film for Wound Dressing Applications
Authors: Jantrawut Pensak , Bunrueangtha Juthamart , Suerthong Juthamart , Kantrong Nutthapong
Published: May 2019

In this study, hydrogel films composed of low methoxyl pectin (LMP), gelatin, and carboxymethyl cellulose (CMC) were fabricated. Glycerin was used as a plasticizer while glutaraldehyde (Glu) and calcium chloride (CaCl2) were used as crosslinking agents in film preparation. Hydrogel films were morphologically characterized and evaluated for mechanical properties. In addition, the investigations for fluid uptake ability, water retention capacity, water vapor transmission rate, and integrity value of the invented films were performed. The results showed that F-Glu-Ca-G30 film demonstrated superior properties when compared to other prepared films. It demonstrated a high percentage of elongation at break (32.80%), fluid uptake ability (88.45% at 2 h), water retention capacity (81.70% at 2 h), water vapor transmission rate (1889 g/m2/day), and integrity value (86.42%). F-Glu-Ca-G30 film was subsequently selected for 10% w/w povidone iodine (PI) loading and tested for anti-Staphylococcus aureus activity using an agar diffusion assay. Notably, F-Glu-Ca-G30-PI film demonstrated a dramatic ability to inhibit microbial growth, when compared to both a blank film and iodine solution control. Our LMP/gelatin/CMC hydrogel film promises to be an effective dressing material with high fluid absorption capacity, fluid holding ability, and water vapor transmission rate. Incorporation of antibiotics such as povidone iodine into the films conferred its antimicrobial property thereby highlighting its potential dermatological use. However, further clinical studies of the application of this hydrogel film as wound dressing material is recommended.
Polyketides from the Mangrove-Derived Endophytic Fungus Cladosporium cladosporioides
Authors: Zhang Fan-Zhong , Li Xiao-Ming , Li Xin , Yang Sui-Qun , Meng Ling-Hong , Wang Bin-Gui
Published: May 2019

Five new polyketides, namely, 5R-hydroxyrecifeiolide (1), 5S-hydroxyrecifeiolide (2), ent-cladospolide F (3), cladospolide G (4), and cladospolide H (5), along with two known compounds (6 and 7), were isolated from the endophytic fungal strain Cladosporium cladosporioides MA-299 that was obtained from the leaves of the mangrove plant Bruguiera gymnorrhiza. The structures of these compounds were established by extensive analysis of 1D/2D NMR data, mass spectrometric data, ECDs and optical rotations, and modified Mosher’s method. The structures of 3 and 6 were confirmed by single-crystal X-ray diffraction analysis and this is the first time for reporting the crystal structures of these two compounds. All of the isolated compounds were examined for antimicrobial activities against human and aquatic bacteria and plant pathogenic fungi as well as enzymatic inhibitory activities against acetylcholinesterase. Compounds 1–4, 6, and 7 exhibited antimicrobial activity against some of the tested strains with MIC values ranging from 1.0 to 64 μg/mL, while 3 exhibited enzymatic inhibitory activity against acetylcholinesterase with the IC50 value of 40.26 μM.
Active Commuting to University and its Association with Sociodemographic Factors and Physical Activity Levels in Chilean Students
Authors: Barranco-Ruiz Yaira , Cruz León Carolina , Villa-González Emilio , Palma Leal Ximena , Chillón Palma , Rodríguez-Rodríguez Fernando
Published: May 2019

Background and Objectives: Active commuting to and from university (ACU) could be a strategy to increase physical activity levels (PA) and promote health in young university students. We aimed to a) examine the patterns of commuting to university in Chilean students; b) the association between the mode of commuting to and from university and socio-demographic factors and PA-levels. Materials and Methods: A total of 496 university students (21.6 ± 2.4 years old) from two universities from Valparaíso (central coast of Chile) participated in this study. Personal data, home address, socio-economic status, PA, and the usual mode of commuting to and from the university were self-reported by a questionnaire. The commute distances were objectively measured using Google-Maps-software. Associations were examined using binary logistic regressions. Results: The main mode of commuting was by bus (to university: 55.2% vs. from university: 59.3%; p < 0.001). The least used mode was cycling (1.4% to and from university). Students living >5-km from university were less active commuters than those living in closer distances: (2–5 km, odds ratio (OR): 4.424, 95% and 95% confidence intervals (CI): 2.443–8.011, p < 0.001; 2 km, OR: 143.052, 95% CI: 55.154–371.030, p < 0.001). Students with low PA-levels were less active commuters than those with medium (OR: 1.446; 95% CI: 0.864–2.421; p = 0.160) or higher levels (OR: 1.880; 95% CI: 1.880–1.094; p = 0.022). Students who lived between 2 and 5 km, presented a significant association to be active commuters when they showed medium PA-levels (OR: 5.244, 95% CI: 1.358–20.246; p = 0.016). Conclusions: Chilean university students from Valparaíso are mainly passive commuters using public transport as the main mode of commuting to and from university; longer distances from home to the university are associated with low PA levels. ACU in distances between 2–5 km (mainly walking) could contribute to having medium PA-levels in Chilean university students. Thus, promoting the ACU walking to and from the university in such distances could be an effective strategy to increase the overall PA levels in Chilean university students.
Nutritional Status and Cardiometabolic Risk Factors in Institutionalized Adults with Cerebral Palsy
Authors: Norte Aurora , Alonso Coral , Martínez-Sanz José Miguel , Gutierrez-Hervas Ana , Sospedra Isabel
Published: May 2019

Background and Objectives: Cerebral palsy (CP) is a set of permanent disorders that limit physical activity and increase the risk of developing other diseases, such as metabolic syndrome (MS). Adequate nutrition can contribute to the prevention of associated symptoms. The main objective of this study is to evaluate the nutritional status and the prevalence of cardiometabolic risk factors in adults with CP and Gross Motor Function Classification System (GMFCS) levels between IV and V. Materials and Methods: A sample of 41 adults with CP and GMFCS levels from IV to V were studied. The variables used in the study were age, sex, weight, height, mean age, and GMFCS level range. To evaluate nutritional status, body mass index and the Mini Nutritional Assessment (MNA), a nutritional screening tool, were used. To assess cardiometabolic risk, data on obesity, central obesity, blood pressure, fasting plasma glucose, total cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, and triglycerides were collected. Results: More than 80% of the population studied was malnourished or at risk of malnutrition, according to the MNA tool classification ranges, and around 35% of the studied population was within the underweight range. Regarding cardiometabolic risk factors, only one adult with CP was diagnosed with MS. Conclusions: The studied population of adults with CP and GMFCS levels between IV and V is not a population at risk of MS; however, the high prevalence of malnutrition, as well as some of the most prevalent cardiovascular risk factors, should be taken into consideration.
Current Pattern of Psychiatric Comorbidity and Psychotropic Drug Prescription in Child and Adolescent Patients
Authors: Araz Altay Mengühan , Bozatlı Leyla , Demirci Şipka Begüm , Görker Işık
Published: May 2019

Background: In recent years, patterns of the use of psychotropic drugs vary with increasing rates of psychiatric presentation and diagnosis in children and adolescents. Purpose: In this study, we aimed to investigate distributions of current psychiatric symptoms and diagnosis, patterns of the use of psychotropic drugs, and differences according to age and gender in patients presented to a child and adolescent outpatient clinic. Methods: All patients aged between 0 and 18 years presenting to a child and adolescent psychiatry outpatient clinic between November 1, 2017 and November 1, 2018 were included in the study. Files of all patients were examined in detail, and patients’ demographic characteristics, symptoms, psychiatric diagnoses established according to the fifth edition of the Diagnostic and Statistical Manual of Mental Disorders (DSM-5), psychotropic drugs initiated, and side effect profiles were recorded. Psychiatric symptoms and diagnostic features of the patients were determined, and the differences were investigated according to gender. Clinical characteristics were compared between diagnosed and undiagnosed patients, and between patients with and without drug initiation. Results: Of the 2066 patients, 1298 (62.8%) were male and the mean age was 10.14 ± 4.42 years. The most common symptoms were hyperactivity (23.8%) and inattention (21.6%) in males, inattention (15.1%) and irritability (14.2%) in females, and 79% of the patients received one or more psychiatric diagnoses. The most common psychiatric diagnoses in both genders were attention-deficit hyperactivity disorder (ADHD), specific learning disorder (SLD), and conduct disorder, respectively. Of the patients who received a psychiatric diagnosis, 61.8% were using psychotropic drugs, with the majority of them (71.3%) receiving monotherapy. The most frequently initiated drugs included psychostimulants, antipsychotics, and antidepressants, with 28.7% of the drug user patients receiving multiple drug therapy. Conclusion: Our study indicates that rate of presentation to child and adolescent psychiatry outpatient clinics is increasing, and rates of diagnosis and initiation of psychiatry drugs are high among the presented children. The prevalence of ADHD shows an increase in males and females in our country, and psychiatric polypharmacy has reached significant rates.
Effects of Cariprazine, Aripiprazole, and Olanzapine on Mouse Fibroblast Culture: Changes in Adiponectin Contents in Supernatants, Triglyceride Accumulation, and Peroxisome Proliferator-Activated Receptor-γ Expression
Authors: Bába László-István , Kolcsár Melinda , Kun Imre Zoltán , Ulakcsai Zsófia , Bagaméry Fruzsina , Szökő Éva , Tábi Tamás , Gáll Zsolt
Published: May 2019

Background and Objectives: The use of the dopamine-partial agonist subclass (also termed dopamine stabilizers) of atypical antipsychotics for the treatment of negative schizophrenia symptoms and some mood disorders has increased recently. Similar to other second-generation antipsychotics (SGAs), aripiprazole (ARI) and cariprazine (CAR) also influence food intake, but the peripheral effects of these drugs on adipose–tissue homeostasis, including adipokine secretion as well as lipo- and adipogenesis, are not fully elucidated. In this study, we explored the adipocyte-related mechanisms induced by second-generation antipsychotics (SGAs), leading to changes in peripheral signals involved in energy homeostasis. Materials and Methods: CAR, a new SGA, was compared with ARI and olanzapine (OLA), using cell cultures to study adipogenesis, and the expression levels of peroxisome proliferator-activated receptor-γ (PPAR-γ) was measured in adipocytes derived from mouse fibroblasts, by western blotting on days 7, 14, and 21 postinduction. The triglyceride (TG) content of the cells was also evaluated on day 15 using Oil Red O staining, and the adiponectin (AN) content in the cell culture supernatants was quantified on days 7 and 15 by enzyme-linked immunosorbent assay. Cells were treated with two concentrations of ARI (0.5 and 20 µg/mL), OLA (1 and 20 µg/mL), and CAR (0.1 and 2 µg/mL). Results: Both concentrations of ARI and OLA, as well as the lower concentration of CAR, significantly increased the TG contents. The AN levels in the supernatants were significantly increased by the higher concentration of ARI on days 7 and 15 (p < 0.05). Although PPAR-γ levels were not significantly affected by ARI and OLA, the lower concentration of CAR induced a significant time-dependent decrease in PPAR-γ expression (p < 0.05). Conclusions: The in vitro adipogenesis considered from TG accumulation, AN secretion, and PPAR-γ expression was differently influenced by ARI, CAR, and OLA. Understanding the adipocyte-related mechanisms of antipsychotics could contribute to understanding their weight-influencing effect.
Materials Properties and Liquid Flow in the Hearth of the Experimental Blast Furnace
Authors: Hu Xianfeng , Sundqvist Ökvist Lena , Ölund Martin
Published: May 2019

The materials’ properties in the hearth of the blast furnace are very crucial for the hearth conditions. In this study, a number of coke, slag, metal, and aggregate samples were collected from the hearth of the LKAB’s experimental blast furnace (EBF). Subsequently, the coke, slag, and metal samples were chemically analyzed by X-ray fluorescence (XRF) or optical emission spectrometer (OES); the aggregate samples were analyzed by scanning electron microscope combined with energy-dispersive X-ray spectroscopy (SEM/EDS). The possible flow field of the liquid in the EBF hearth before quenching is depicted according to Cu tracers in the metal samples. Selected elements in the coke, slag, and metal were mapped for two sampling layers in the hearth, as well as in one cross section of the flow field. The results indicate that there exists an area beneath, and in front of, tuyere 3, where the flow resistance of the liquid was high. The high flow resistance contributed to the formation of a cold zone in the close-to-wall region and at the bottom of the EBF hearth. The temperature distribution in the EBF hearth has significant impacts on the chemical properties of the materials in different positions of the EBF hearth, as well as on the radial and vertical distributions of certain elements/components.
Sparse 3D Seismic Imaging in the Kylylahti Mine Area, Eastern Finland: Comparison of Time Versus Depth Approach
Authors: Singh Brij , Malinowski Michał , Hloušek Felix , Koivisto Emilia , Heinonen Suvi , Hellwig Olaf , Buske Stefan , Chamarczuk Michał , Juurela Sanna
Published: May 2019

A 10.5 km2 3D seismic survey was acquired over the Kylylahti mine area (Outokumpu mineral district, eastern Finland) as a part of the COGITO-MIN (COst-effective Geophysical Imaging Techniques for supporting Ongoing MINeral exploration in Europe) project, which aimed at the development of cost-effective geophysical imaging methods for mineral exploration. The cost-effectiveness in our case was related to the fact that an active-source 3D seismic survey was accomplished by using the receiver spread originally designed for a 3D passive survey. The 3D array recorded Vibroseis and dynamite shots from an active-source 2D seismic survey, from a vertical seismic profiling experiment survey, as well as some additional “random” Vibroseis and dynamite shots made to complement the 3D source distribution. The resulting 3D survey was characterized by irregular shooting geometry and relatively large receiver intervals (50 m). Using this dataset, we evaluate the effectiveness of the standard time-imaging approach (post-stack and pre-stack time migration) compared to depth imaging (standard and specialized Kirchhoff pre-stack depth migration, KPreSDM). Standard time-domain processing and imaging failed to convincingly portray the first ~1500 m of the subsurface, which was the primary interest of the survey. With a standard KPreSDM, we managed to obtain a good image of the base of the Kylylahti formation bordering the extent of the mineralization-hosting Outokumpu assemblage rocks, but otherwise the image was very noisy in the shallower section. The specialized KPreSDM approach (i.e., coherency-based Fresnel volume migration) resulted in a much cleaner image of the shallow, steeply dipping events, as well as some additional deeper reflectors, possibly representing repetition of the contact between the Outokumpu assemblage and the surrounding Kalevian metasediments at depth.
Investigation of Size-Dependent Sublimation Kinetics of 2,4,6-Trinitrotoluene (TNT) Micro-Islands Using In Situ Atomic Force Microscopy
Authors: Lee Yong Joon , Weeks Brandon L.
Published: May 2019

Kinetic thermal analysis was conducted using in situ atomic force microscopy (AFM) at a temperature range of 15–25 °C to calculate the activation energy of the sublimation of 2,4,6-trinitrotoluene (TNT) islands. The decay of different diameter ranges (600–1600 nm) of TNT islands was imaged at various temperatures isothermally such that an activation energy could be obtained. The activation energy of the sublimation of TNT increases as the diameter of islands increases. It was found that the coarsening and the sublimation rate of TNT islands can be determined by the local environment of the TNT surface. This result demonstrates that a diffusion model cannot be simply applied to “real world” systems for explaining the sublimation behavior and for estimating the coarsening of TNT.
N1-Propargylguanosine Modified mRNA Cap Analogs: Synthesis, Reactivity, and Applications to the Study of Cap-Binding Proteins
Authors: Kopcial Michal , Wojtczak Blazej A. , Kasprzyk Renata , Kowalska Joanna , Jemielity Jacek
Published: May 2019

The mRNA 5′ cap consists of N7-methylguanosine bound by a 5′,5′-triphosphate bridge to the first nucleotide of the transcript. The cap interacts with various specific proteins and participates in all key mRNA-related processes, which may be of therapeutic relevance. There is a growing demand for new biophysical and biochemical methods to study cap–protein interactions and identify the factors which inhibit them. The development of such methods can be aided by the use of properly designed fluorescent molecular probes. Herein, we synthesized a new class of m7Gp3G cap derivatives modified with an alkyne handle at the N1-position of guanosine and, using alkyne-azide cycloaddition, we functionalized them with fluorescent tags to obtain potential probes. The cap derivatives and probes were evaluated in the context of two cap-binding proteins, eukaryotic translation initiation factor (eIF4E) and decapping scavenger (DcpS). Biochemical and biophysical studies revealed that N1-propargyl moiety did not significantly disturb cap–protein interaction. The fluorescent properties of the probes turned out to be in line with microscale thermophoresis (MST)-based binding assays.