bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Anolyte on S. Typhimurium and L. monocytogenes Growth in Minced Pork and Beef Cuts
Authors: Riešutė Reda , Šalomskienė Joana , Šalaševičienė Alvija , Mačionienė Irena
Published: January 2022

In this paper, anolyte is considered as a possible disinfectant for inhibiting the growth of bacteria in meat (beef cuts and minced pork). Meat cuts were contaminated with two concentrations of L. monocytogenes and S. Typhimurium, as these are the most common meat pathogens that are closely regulated by the EU, and treated with two different concentrations of anolyte: 20% for beef cuts and 18% for minced pork. Then, the total viable count (TVC), L. monocytogenes count and S. Typhimurium count were determined. In meat cuts and minced pork, anolyte was able to reduce TVC, S. Typhimurium and L. monocytogenes counts effectively, significantly decreasing L. monocytogenes and S. Typhimurium counts after spraying and throughout 29 days of incubation at 0–4 °C. TVC was reduced after spraying and for 10 days of incubation but later increased to be the same as before spraying with anolyte. Anolyte was effective when spraying beef cuts with a 20% solution for 60 s against pathogenic bacteria L. monocytogenes and Salmonella spp. and also when using it at a concentration of 18% from the minced meat mass. Initially, anolyte significantly decreased TVC, however during the storage period (10–29 days) TVC increased but remained significantly lower compared to control. Anolyte was effective in reducing L. monocytogenes and S. Typhimurium counts throughout the study, and after 29 days of incubation, these bacteria could not be detected in the samples treated with anolyte.
Disintegrating the Structure and Improving the Functionalities of Pea Fiber by Industry-Scale Microfluidizer System
Authors: He Xiaohong , Dai Taotao , Sun Jian , Liang Ruihong , Liu Wei , Chen Mingshun , Chen Jun , Liu Chengmei
Published: January 2022

In the food industry, the most prominent and concerned points in the application of dietary fiber are hydration properties and oil absorption capacity. The target of this work was to investigate the impact of a novel industry-scale microfluidizer system (ISMS) on the changing structures and functionalities of pea fiber. Different ISMS treatment intensity (0–120 MPa for one pass and 120 MPa for two passes) was applied to treat pea fiber. ISMS treatment induced the reduction in particle size and the transformation of big compact blocks to loose flakes, and the destruction of the original ordered cellulose structure caused the decline of crystallinity. Meanwhile, the hydration properties of pea fiber were improved, and pre-pulverizer and industry-scale microfluidizer treatment together increased the swelling capacity and water retention capacity of fiber. The oil holding capacity of ISMS-treated fiber was increased to more than double the original one. The elevated functionalities of pea fiber by ISMS treatment could be attributed to loosening structure, exposing more surface area, and disordering the crystalline structure, which increased the sites of water binding and oil adsorption. These findings suggested that ISMS could be applied as an effective industrial technique to the disintegrate structure and improve the functionalities of pea fiber, so as to widen the application of pea fibers in foods.
Preparation, Characterization, and Biological Features of Cactus Coated Bacterial Cellulose Hydrogels
Authors: Kamal Tahseen , Ul-Islam Mazhar , Khan Sher Bahadar , Bakhsh Esraa M. , Chani Muhammad Tariq Saeed
Published: January 2022

The current study was aimed at developing BC-Cactus (BCC) composite hydrogels with impressive mechanical features for their potential applications in medical and environmental sectors. BCC composites hydrogels were developed through cactus gel coating on a never dried BC matrix. The FE-SEM micrographs confirmed the saturation of BC fibrils with cactus gel. Additionally, the presence of various functional groups and alteration in crystalline behavior was confirmed through FTIR and XRD analysis. Mechanical testing illustrated a three-times increase in the strain failure and an increase of 1.6 times in the tensile strength of BCC composite. Absorption capabilities of BCC were much higher than pure BC and it retained water for a longer period of time. Additionally, the rewetting and absorption potentials of composites were also higher than pure BC. The composite efficiently adsorbed Pb, Zn, Cu, and Co metals. Biocompatibility studies against human HaCat cell line indicated much better cell adhesion and proliferation of BCC compared to BC. These findings advocate that the BCC composite could find applications in medical, pharmaceutical and environmental fields.
Impact of Sequential Lipid Meals on Lymphatic Lipid Absorption and Transport in Rats
Authors: Zhu Qi , Yang Qing , Shen Ling , Qu Jie , Xu Meifeng , Wang David Q.-H. , Tso Patrick , Liu Min
Published: January 2022

The sequential meal pattern has recently received more attention because it reflects a phycological diet style for human beings. The present study investigated the effects of the second lipid meal on lymphatic lipid absorption and transport in adult rats following a previous lipid meal. Using the well-established lymph fistula model, we found that the second lipid meal significantly increased the lymphatic output of triglycerides, cholesterol, phospholipids, and non-esterified fatty acids compared with a single lipid meal. Besides that, the time reaching the peak of each lipid output was significantly faster compared with the first lipid meal. Additionally, the second lipid meal significantly increased the lymphatic output of apolipoprotein A-IV (ApoA-IV), but not apolipoprotein B-48 (ApoB-48) or apolipoprotein A-I (ApoA-I). Interestingly, the triglyceride/apoB-48 ratio was significantly increased after the second lipid meal, indicating the increased chylomicron size in the lymph. Finally, the second lipid meal increased the lymphatic output of rat mucosal mast cell protease II (RMCPII). No change was found in the expression of genes related to the permeability of lymphatic lacteals, including vascular endothelial growth factor-A (Vegfa), vascular endothelial growth factor receptor 1 (Flt1), and Neuropilin1 (Nrp1). Collectively, the second lipid meal led to the rapid appearance of bigger-sized chylomicrons in the lymph. It also increased the lymphatic output of various lipids and apoA-IV, and mucosal mast cell activity in the intestine.
Behavior Problems and Social Competence in Fragile X Syndrome: A Systematic Review
Authors: Cregenzán-Royo Olga , Brun-Gasca Carme , Fornieles-Deu Albert
Published: January 2022

Fragile X syndrome (FXS) causes intellectual disability and is the known leading cause of autism. Common problems in FXS include behavior and social problems. Along with syndromic characteristics and autism comorbidity, environmental factors might influence these difficulties. This systematic review focuses on the last 20 years of studies concerning behavior and social problems in FXS, considering environmental and personal variables that might influence both problems. Three databases were reviewed, leading to fifty-one studies meeting the inclusion criteria. Attention deficit hyperactivity disorder (ADHD) problems remain the greatest behavior problems, with behavioral problems and social competence being stable during the 20 years. Some developmental trajectories might have changed due to higher methodological control, such as aggressive behavior and attention problems. The socialization trajectory from childhood to adolescence remains unclear. Comorbidity with autism in individuals with FXS increased behavior problems and worsened social competence profiles. At the same time, comparisons between individuals with comorbid FXS and autism and individuals with autism might help define the comorbid phenotype. Environmental factors and parental characteristics influenced behavior problems and social competence. Higher methodological control is needed in studies including autism symptomatology and parental characteristics. More studies comparing autism in FXS with idiopathic autism are needed to discern differences between conditions.
Reconstructing the Variscan Terranes in the Alpine Basement: Facts and Arguments for an Alpidic Orocline
Authors: Faure Michel , Ferrière Jacky
Published: January 2022

The existence of pieces of the Variscan belt in the Alpine basement has been acknowledged for a long time but the correlation of these massifs to the litho-tectonic domains established in Western Europa outside the Alpine chain is still disputed. Due to their ubiquitous character, the abundant late Variscan migmatites and granites are useless to reconstruct the Variscan architecture in the Alpine basement. Ophiolitic sutures, high- and low-grade metamorphic units, and foreland basins provide a preliminary reconstruction of the Variscan orogen exposed in the Alpine basement. The longitudinal extension of the Armorican and Saxo-Thuringian microcontinents between Laurussia and Gondwana is proposed independently of the Intra-alpine and Galatian terranes. The litho-tectonic units of the Corsica-Sardinia segment are correlated to the Moldanubian, Armorican and Saxo-Thuringian Domains. In the Alpine Helvetic and Penninic Domains, the Chamrousse ophiolites are ascribed to the Tepla-Le Conquet suture, whereas the Lepontine, and Stubach ophiolites represent the Rheic suture. The south-directed nappe stack of the South Alpine Domain is similar to the Moldanubian French Massif Central. In the Austroalpine nappe stack, the Ritting ophiolites separate Saxo-Thuringia and Armorica continental blocks. Disentangling the Variscan belt in the Alpine basement suggests a concave-to-the-East arcuate structure called here the Variscan Alpidic orocline.
Effect of Elevated CO2 during Low Temperature Storage on the Quality Attributes of Cut Spearmint
Authors: Sommano Sarana Rose , Khamsaw Pattarapol , Van Doan Hien , Cheewangkoon Ratchadawan , Amodio Maria Luisa , De Chiara Maria Lucia Valeria , Mastrandrea Leonarda , Pati Sandra , Colelli Giancarlo
Published: January 2022

The effect of elevated CO2 in a controlled atmospheric condition (CA) on the quality attributes of fresh-cut spearmint (Mentha spicata) during refrigerated storage is investigated in the present study. Cut stems of spearmint were exposed to the continuous flow of humidified air enriched with 0 (as a control), 5, 10 and 20% CO2 during storage at 5 °C. Weight loss, leaf colour, total phenols, antioxidant activity, aromatic profile, ascorbic acid, ethanol, ammonia and ethanol-acetaldehyde concentrations were measured before and after storage for 5, 10 and 14 days. Over time, CO2 treatments increased the weight loss, surface colour, L* (from white to black) and b* (from blue to yellow) values, but lowered a* (from green to red). When compared to fresh spearmint, the lowest CO2 concentration was able to maintain the overall colour variations. The 20% CO2 treatment showed significant declines in the total phenolic content, antioxidant potential and low appearance score after 10 days, thus its quality assessment was terminated. Vitamin C levels decreased with time in all the treatments, although the 10% and 20% CO2 treatments had the lowest levels. The toxicity of the cell structures detected by the ammonia content increased and was significantly higher in all CO2 treatments. Storage in the CA with the gas composition at 5% preserved the aromatic profiles similar to those stored in air. In conclusion, increased CO2 did not improve the storability of fresh cut spearmint held at low temperatures, and the 20% gas composition had a significant negative impact on the visual quality.
Axillary Bud Fate Shapes Plant Architecture in Horticultural Crops
Authors: Andrés Javier , Koskela Elli
Published: January 2022

Above-ground plant architecture is dictated to a large extent by the fates and growth rates of aerial plant meristems. Shoot apical meristem gives rise to the fundamental plant form by generating new leaves. However, the fates of axillary meristems located in leaf axils have a great influence on plant architecture and affect the harvest index, yield potential and cultural practices. Improving plant architecture by breeding facilitates denser plantations, better resource use efficiency and even mechanical harvesting. Knowledge of the genetic mechanisms regulating plant architecture is needed for precision breeding, especially for determining feasible breeding targets. Fortunately, research in many crop species has demonstrated that a relatively small number of genes has a large effect on axillary meristem fates. In this review, we select a number of important horticultural and agricultural plant species as examples of how changes in plant architecture affect the cultivation practices of the species. We focus specifically on the determination of the axillary meristem fate and review how plant architecture may change even drastically because of altered axillary meristem fate. We also explain what is known about the genetic and environmental control of plant architecture in these species, and how further changes in plant architectural traits could benefit the horticultural sector.
Spatial Pattern and Spillover of Abatement Effect of Chinese Environmental Protection Tax Law on PM2.5 Pollution
Authors: Han Fei , Li Junming
Published: January 2022

Particulate matter (PM2.5) pollution is a threat to public health, and environmental taxation is an important regulatory mode controlling PM2.5 pollution. In 2018, China implemented the Environmental Protection Tax Law (EPTL) targeting PM2.5 pollution. Based on in-situ monitoring and emission inventory data, a Bayesian hierarchical spatiotemporal model combining a two-period trends difference method was employed to measure the abatement effects of China’s EPTL on PM2.5 pollution (AEEPTLPM). On this basis, a spatial spillover index (SSI) of the AEEPTLPM is proposed. Applying this index, we calculated the spatial spillover characteristics of the AEEPTLPM in mainland China at a provincial scale in 2018–2019. The results show that the EPTL has had significant abatement effects on both in-situ-monitored PM2.5 concentrations and local total industrial PM2.5 emissions. Additionally, the two types of AEEPTLPM display distinct spatial heterogeneity. A correlation between the AEEPTLPM and the degree of PM2.5 pollution was observed; areas with serious PM2.5 pollution have higher AEEPTLPM levels, and vice versa. The SSI indicates that the AEEPTLPM exhibits significant spatial spillover characteristics, and spatial heterogeneity is also present.
Extreme Longevity: Analysis of the Direct or Indirect Influence of Environmental Factors on Old, Nonagenarians, and Centenarians in Cilento, Italy
Authors: Aliberti Silvana Mirella , De Caro Francesco , Funk Richard H. W. , Schiavo Luigi , Gonnella Joseph , Boccia Giovanni , Capunzo Mario
Published: January 2022

Longevity is rightly considered one of the greatest achievements of modern society, an achievement understood as the possibility of increasing the healthy part of life and not only its full duration. This study investigated the phenomenon of regional longevity in Cilento by analyzing the association between longevity indicators and some environmental factors, in order to understand if factors such as altitude, climate, UNESCO protected areas, and hinterland can directly or indirectly influence the measure of healthy living and lead to longevity. Demographic and environmental data were collected through the Archives of the Cilento municipalities, the National Institute of Statistics, the Italian Institute for Environmental Protection and Research, and the Italian National Commission for UNESCO. The Geographically Weighted Regression were used to determine the association between longevity indicators and environmental factors. Correlation analysis between the longevity indicators was investigated in order to have a complete picture of longevity in Cilento. It was discovered that Cilento longevity is mainly found in the central area of the territory and from there, by age groups, it widens towards the south-eastern area. This designated area is part of the hilly area of the Cilento, at an altitude between 400 and 700 m above sea level. The towns of this area are part of the UNESCO heritage and are characterized by a transitional climate between Mediterranean and temperate climate. Moreover, the correlation analysis between the six indicators of longevity has confirmed a linear relationship between the six variables and this indicates that in the years to come there will be the probabilities of a generational turn over between the old, great old, nonagenarians and current centenarians, provided that the SARS-CoV-2 pandemic subsides.
Is the Xiaomi Mi Band 4 an Accuracy Tool for Measuring Health-Related Parameters in Adults and Older People? An Original Validation Study
Authors: de la Casa Pérez Ana , Latorre Román Pedro Ángel , Muñoz Jiménez Marcos , Lucena Zurita Manuel , Laredo Aguilera José Alberto , Párraga Montilla Juan Antonio , Cabrera Linares José Carlos
Published: January 2022

Background: The aim of this study was to analyse the validity and accuracy of the low-cost Xiaomi Mi Band 4 (MB4) fitness tracker in relation to step count and heart rate in free-living conditions. Methods: 46 participants join in this study (38.65 ± 14.36 years old). The MB4 was compared with a video recording in laboratory conditions, also with the Sense Wear and Firstbeat monitors in free-living conditions. Results: No significant differences were found in the number of steps in the laboratory protocol between devices, in both, at low and high walking speed. For the free-living conditions, the MB4 showed high values of convergent validity in relation to the Firstbeat monitor during recording in both rest and walking situations. Moreover, the MB4 showed adequate values of convergent validity with the Sense Wear monitor during the 24 h recording, at medium speeds, and climbing stairs. Conclusion: The accuracy and precision of the MB4 is reasonable and can be used to monitor the average of step count and heart rate in free-living conditions.
A Risk-Based Approach in Rehabilitation of Water Distribution Networks
Authors: Raspati Gema Sakti , Bruaset Stian , Bosco Camillo , Mushom Lars , Johannessen Birgitte , Ugarelli Rita
Published: January 2022

A risk-based approach to support water utilities in terms of defining pipe rehabilitation priorities is presented. In a risk analysis in the risk management process, the probability that a given event will happen and the consequences if it does happen have to be estimated and combined. In the quantitative risk analysis, numerical values are assigned to both consequence and probability. In this study, the risk event addressed was the inability to supply water due to pipe breaks. Therefore, on the probability side, the probability of pipes breaking was assessed, and on the consequence side, the reduced ability to satisfy the water demand (hydraulic reliability) due to pipe breakage was computed. Random Forest analysis was implemented for the probability side, while the Asset Vulnerability Analysis Toolkit was used to analyse the network’s hydraulic reliability. Pipes could then be ranked based on the corresponding risk magnitude, thereby feeding a risk evaluation step; at this step, decisions are made concerning which risks need treatment, and also concerning the treatment priorities, i.e., rehabilitation priorities. The water distribution network of Trondheim, Norway, was used as a case study area, and this study illustrates how the developed method aids the development of a risk-based rehabilitation plan.
Landscape Pattern and Ecological Risk Assessment in Guangxi Based on Land Use Change
Authors: Yang Yanping , Chen Jianjun , Lan Yanping , Zhou Guoqing , You Haotian , Han Xiaowen , Wang Yu , Shi Xue
Published: January 2022

Due to ecological environmental fragility and soil erosion in Guangxi, studies of landscape patterns and associated ecological risks are needed to guide sustainable land development and ecologically sensitive land management. This study assesses dynamic spatial and temporal change patterns in land use and ecological risks based on 30 m land-use data, analyzes spatial correlations with ecological risks, and explores natural and socio-economic factor impacts on ecological risks. The results reveal: (1) A rapid and sizeable construction land increase in Guangxi from 2000 to 2018 associated mainly with loss of woodland and grassland. (2) Guangxi had the highest number of arable land patches from 2000 to 2018, and the distribution tended to be fragmented; moreover, the construction land gradually expanded outward from concentrated areas to form larger aggregates with increasing internal stability each year. (3) Guangxi ecological risk levels were low, low–medium, and medium, with significantly different spatial distributions observed for areas possessing different ecological risk levels. Regional ecological risk gradually decreased from the middle Guangxi regions to the surrounding areas and was positively correlated with spatial distribution. (4) Socio-economic factor impacts on ecological risk exceeded natural factor impacts. These results provide guidance toward achieving ecologically sensitive regional land-use management and ecological risk reduction and control, it can also provide a reference for ecological risk research in other similar regions in the world.
Direct Healthcare Costs of Moderate and Severe Work-Related Injuries: Estimates from the National Trauma Center of Qatar
Authors: Consunji Rafael J. , Mekkodathil Ahammed , El-Menyar Ayman , Mehmood Amber , Sathian Brijesh , Hyder Adnan A. , Hirani Nazia , Abeid Aisha , Al-Thani Hassan , Peralta Ruben
Published: January 2022

Work-related injuries (WRIs) are recognized as a leading cause of admission to the national trauma center of Qatar. A retrospective analysis of trauma registry data and electronic medical records was conducted on a cohort of all WRI patients who were admitted to the Hamad Trauma Center (HTC), in Doha, Qatar, between 2011 and 2017. A total of 3757 WRI patients were treated at the HTC over the 7-year study period. The overall cost for treatment was 124,671,431 USD (18 million USD per year), with a median cost of 19,071 USD. There was a strong positive correlation between the overall cost and hospital-stay cost (r2 = 0.949, p = 0.00001) and between the overall cost and procedure cost (r2 = 0.852, p = 0.00001). Motor vehicle crash (MVC) victims who wore seatbelts had significantly lower injury severity, hospital stay and median total costs. A comparison of patients by quartiles of the costs incurred showed that the proportions of MVC victims, pedestrian injuries and mortality were significantly higher in the fourth quartile when compared to other quartiles (p < 0.05). These findings suggest that investments in the primary prevention of work-related injuries from falls and MVCs, through proven interventions, should be priorities for occupational safety and health in Qatar.
Family Support and Social Support Associated with National Essential Public Health Services Utilization among Older Migrants in China: A Gender Perspective
Authors: Cao Wangnan , Yun Qingping , Chang Chun , Ji Ying
Published: January 2022

China provides National Essential Public Health Services (NEPHS) free of charge to all citizens to ensure access to essential health services. The present study aimed to explore the associations between different sources of support and NEPHS service utilization among older migrants in China with a gender perspective. We used a national cross-sectional dataset derived from the 2015 China Migrants Dynamic Survey. Participants were included if they were aged ≥60 years and without household registration at the residence. Among 1989 participants, 35.2% enrolled in a free physical examination in the past year: 34.6% for males and 35.9% for females. Among male participants, having more local friends (OR = 1.47, 95% CI: 1.09, 1.99) and having insurance at the residence (OR = 1.75, 95% CI: 1.03, 2.96) were associated with enrolment in a free physical examination after controlling for age, education, and self-reported health status. Two additional factors, marital status and family structure, were found for female participants to be associated with enrolment in a free physical examination. NEPHS service utilization was far from satisfactory among older migrants in China, and both family support and social support played a role in it. There are common and unique factors associated with NEPHS service utilization in terms of gender.
Distress among Korean Cancer Survivors: A Latent Profile Analysis
Authors: Park Kwang-Hi , Song Min Kyung
Published: January 2022

This study aimed to classify cancer survivors’ latent profile analysis (LPA) according to the problem list and identify the differences in distress between subgroups. Furthermore, this study identified differences between subgroups based on their demographic and clinical characteristics. A self-reported cross-sectional survey was administered to 446 adult cancer survivors in Korea. A distress thermometer and problem list were used, and four domains of the problem list were used to perform LPA and create subgroups. Quade’s non-parametric analysis of covariance was used to determine the difference in distress between the profiles. The three identified subgroups of the problem list were: “low problem group” (36.7%), “high problem group” (49.1%), and “family only low problem group” (14.2%). The analysis showed that there was a difference in the distress level according to the sub-profile of the problem list (F = 43.69, p < 0.001). In interventions for distress, integrative interventions that are not limited to one area are necessary, and cultural characteristics as well as the problem list relevant to cancer survivors should be considered.
Spatiotemporal Analysis for COVID-19 Delta Variant Using GIS-Based Air Parameter and Spatial Modeling
Authors: Cahyadi Mokhamad Nur , Handayani Hepi Hapsari , Warmadewanthi IDAA , Rokhmana Catur Aries , Sulistiawan Soni Sunarso , Waloedjo Christrijogo Sumartono , Raharjo Agus Budi , Endroyono  , Atok Mohamad , Navisa Shilvy Choiriyatun , Wulansari Mega , Jin Shuanggen
Published: January 2022

The coronavirus disease of 2019 (COVID-19) pandemic is currently a global challenge, with 210 countries, including Indonesia, seeking to minimize its spread. Therefore, this study aims to determine the spatiotemporal spread pattern of this virus in Surabaya using various data on confirmed cases from 28 April to 26 October 2021. It also aims to determine the relationship between pollutant parameters, such as carbon monoxide (CO), nitrogen dioxide (NO2), sulfur dioxide (SO2), and ozone (O3), as well as the government’s high social restrictions policy in Java-Bali. Several methods, such as the weighted mean center, directional distribution, Getis–Ord Gi*, Moran’s I, and geographically weighted regression, were used to identify the spatial spread pattern of the virus. The weighted mean center indicated that the epicenter location of the outbreak moved randomly. The directional distribution demonstrated a decrease of 21 km2 at the end of the study phase, which proved that its spread has significantly reduced in Surabaya. Meanwhile, the Getis–Ord Gi* results demonstrated that the eastern and southern parts of the study region were highly infected. Moran’s I demonstrate that COVID-19 cases clustered during the spike. The geographically weighted regression model indicated a number of influence zones in the northeast, northwest, and a few in the southwest parts at the peak of R2 0.55. The relationship between COVID-19 cases and air pollution parameters proved that people living at the outbreak’s center have low pollution levels due to lockdown. Furthermore, the lockdown policy reduced CO, NO2, SO2, and O3. In addition, increase in air pollutants; namely, NO2, CO, SO2 and O3, was recorded after 7 weeks of lockdown implementation (started from 18 August).
Trends of Water, Sanitation, and Hygiene (WASH) Research in Indonesia: A Systematic Review
Authors: Satriani S. , Ilma Izana Saffana , Daniel D.
Published: January 2022

This study provides an overview of water, sanitation, and hygiene (WASH) research trends in Indonesia from 1975 until April 2021. The systematic review compiled 272 articles related to the Sustainable Development Goals 6.1 and 6.2 in Indonesia, which were published in the Web of Science and Scopus databases. The results showed that the water-related topic (41%) was discussed more often than sanitation (22%) or hygiene (13%). Furthermore, the social theme (39%) was dominantly found in all these articles, mostly finding determinants of WASH-related behavior. However, few WASH implementation studies or behavioral change interventions were recorded in Indonesia, suggesting a gap between science and policy or implementation. On the other hand, hygiene-related topics (14%) and WASH-related financial themes (6%) were the least studied in Indonesia. Combinations of topics (23%) and themes (15%) were also often conducted in Indonesia, suggesting that WASH researchers started to recognize the need to analyze WASH problems holistically, i.e., from multiple perspectives. In addition, the distribution of WASH research was still dominated in the central part of Indonesia, whereas the WASH-related problems, i.e., poor WASH services, and behavior, often occur in this area. This study also offers some research gaps, both in terms of topics, themes, and regional distribution, that need to be considered for the design of future WASH research in Indonesia.
Psychological Effects of Online-Based Mindfulness Programs during the COVID-19 Pandemic: A Systematic Review of Randomized Controlled Trials
Authors: Yeun Young-Ran , Kim Sang-Dol
Published: January 2022

(1) Background: The COVID-19 outbreak has caused psychological problems worldwide. This review explored the psychological effects of online-based mindfulness programs during the COVID-19 pandemic. (2) Methods: This systematic review was guided by the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. Randomized controlled trials that were published in the English language from 1 January 2020 to 31 May 2021 on online-based mindfulness programs for psychological problems due to the COVID-19 pandemic were searched in electronic databases. Quality assessment was conducted on the retrieved RCTs using the Cochrane risk of bias tool for RCTs. (3) Results: Six RCTs were included in this review. Quality appraisal of included RCTs ranged from 1 for low risk of bias to 5 for high risk of bias. There is evidence from the six RCTs that online-based mindfulness interventions may have favorable effects for reducing the levels of psychological problems, such as anxiety, depression, and stress. (4) Conclusions: Online-based mindfulness programs may be used as complementary interventions for clinical populations, healthy individuals, and healthcare workers with psychological problems due to the COVID-19 pandemic.
Liraglutide + PYY3-36 Combination Therapy Mimics Effects of Roux-en-Y Bypass on Early NAFLD Whilst Lacking-Behind in Metabolic Improvements
Authors: Metzner Valentin , Herzog Gloria , Heckel Tobias , Bischler Thorsten , Hasinger Julia , Otto Christoph , Fassnacht Martin , Geier Andreas , Seyfried Florian , Dischinger Ulrich
Published: January 2022

Background: Treatment options for NAFLD are still limited. Bariatric surgery, such as Roux-en-Y gastric bypass (RYGB), has been shown to improve metabolic and histologic markers of NAFLD. Glucagon-like-peptide-1 (GLP-1) analogues lead to improvements in phase 2 clinical trials. We directly compared the effects of RYGB with a treatment using liraglutide and/or peptide tyrosine tyrosine 3-36 (PYY3-36) in a rat model for early NAFLD. Methods: Obese male Wistar rats (high-fat diet (HFD)-induced) were randomized into the following treatment groups: RYGB, sham-operation (sham), liraglutide (0.4 mg/kg/day), PYY3-36 (0.1 mg/kg/day), liraglutide+PYY3-36, and saline. After an observation period of 4 weeks, liver samples were histologically evaluated, ELISAs and RNA sequencing + RT-qPCRs were performed. Results: RYGB and liraglutide+PYY3-36 induced a similar body weight loss and, compared to sham/saline, marked histological improvements with significantly less steatosis. However, only RYGB induced significant metabolic improvements (e.g., adiponectin/leptin ratio 18.8 ± 11.8 vs. 2.4 ± 1.2 in liraglutide+PYY3-36- or 1.4 ± 0.9 in sham-treated rats). Furthermore, RNA sequencing revealed a high number of differentially regulated genes in RYGB treated animals only. Conclusions: The combination therapy of liraglutide+PYY3-36 partly mimics the positive effects of RYGB on weight reduction and on hepatic steatosis, while its effects on metabolic function lack behind RYGB.
Short- and Mid-Term Survival of Geriatric Patients with Septic Arthritis of the Knee and the Impact of Risk Factors on Survival
Authors: Haag Nina Pauline , Geßlein Markus , Millrose Michael , Ziegler Renate , Willauschus Maximilian , Steinmann Jörg , Bail Hermann Josef , Rüther Johannes
Published: January 2022

Septic arthritis is common in older adults and can be related to joint surgery or hematogenous distribution. To date, the risk factors affecting survival are unknown. This study aimed to evaluate the effects of existing implants, positive synovial microbiological culture results, and the American Society of Anesthesiology Physical Status (ASA) classification on the short- and mid-term survival of older patients with primary septic gonarthritis. This retrospective study included 133 older adults >60 years who underwent surgery for primary septic gonarthritis. Data were collected from medical records and public obituaries. Kaplan–Meier survival curves were used to estimate the probability of survival, as well as log-rank tests to measure and compare survival rates over one- and five-year periods. The mean age was 74.9 years (SD ± 9.2), and the 5-year follow-up rate was 74.3% (the mean follow-up was 3000.5 days; SD ± 1771.6). Mean survival was significantly different in patients with implants and without implants (p = 0.015), and between ASA II, ASA III, and ASA IV (p < 0.001). There was no significant difference in the survival of patients with or without a positive synovial microbiological culture (p = 0.08). Older adults with septic monoarthritis and pre-existing medical implants showed impaired survival. The ASA classification prior to surgery for primary septic monoarthritis can be helpful in identifying patients with poorer mid-term outcomes.
Effect of a Craniosacral Therapy Protocol in People with Migraine: A Randomized Controlled Trial
Authors: Muñoz-Gómez Elena , Inglés Marta , Aguilar-Rodríguez Marta , Mollà-Casanova Sara , Sempere-Rubio Núria , Serra-Añó Pilar , Espí-López Gemma V.
Published: January 2022

Background: Migraine is a common neurological disorder, and it is the second leading cause of disability worldwide. Manual techniques based on physical therapy have been proposed to improve migraine aspects; however, further research is needed on their effectiveness. The aim of this study was to evaluate the effectiveness of a craniosacral therapy protocol on different features in migraine patients. Methods: Fifty individuals with migraine were randomly divided into two groups (n = 25 per group): (i) craniosacral therapy group (CTG), following a craniosacral therapy protocol, and (ii) sham control group (SCG), with a sham treatment. The analyzed variables were pain, migraine severity and frequency of episodes, functional, emotional, and overall disability, medication intake, and self-reported perceived changes, at baseline, after a 4 week intervention, and at 8 week follow-up. Results: After the intervention, the CTG significantly reduced pain (p = 0.01), frequency of episodes (p = 0.001), functional (p = 0.001) and overall disability (p = 0.02), and medication intake (p = 0.01), as well as led to a significantly higher self-reported perception of change (p = 0.01), when compared to SCG. In addition, the results were maintained at follow-up evaluation in all variables. Conclusions: A protocol based on craniosacral therapy is effective in improving pain, frequency of episodes, functional and overall disability, and medication intake in migraineurs. This protocol may be considered as a therapeutic approach in migraine patients.
Serum Sulfatide Levels as a Biomarker of Active Glomerular Lesion in Patients with Anti-Neutrophil Cytoplasmic Antibody-Associated Vasculitis: A Single Center Pilot Study
Authors: Harada Makoto , Nakajima Takero , Yamada Yosuke , Aomura Daiki , Yamaguchi Akinori , Sonoda Kosuke , Tanaka Naoki , Hashimoto Koji , Kamijo Yuji
Published: January 2022

Sulfatides are glycosphingolipids that are associated with coagulation and platelet aggregation. Anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) activates platelet function and often leads to thrombotic complications. These facts suggest an association between serum sulfatides and AAV. We aimed to clarify the significance of serum sulfatide levels in patients with AAV. We conducted a retrospective, single-center, observational pilot study that included 35 patients who developed AAV and 10 control patients who were candidates for living-donor kidney transplantation. We compared serum sulfatide levels between the control and AAV patients. We analyzed the differences in serum sulfatide levels among four classes (focal, crescentic, mixed, and sclerotic class) of glomerular lesions that were categorized by histopathologic classification of ANCA-associated glomerulonephritis. Serum sulfatide levels in patients with AAV were significantly lower than those in the controls. Serum sulfatide levels were significantly different between the four classes. Additionally, serum sulfatide levels in the crescentic class were significantly lower than those in the other classes. Serum sulfatide levels were significantly correlated with albumin, cholesterol, C-reactive protein, and pentraxin 3. In conclusion, serum sulfatide levels are significantly correlated with inflammation, reflecting crescentic glomerulonephritis, which is an active glomerular lesion in AAV patients.
Fatty Pancreas and Pancreatic Cancer: An Overlooked Association?
Authors: Khoury Tawfik , Sbeit Wisam
Published: January 2022

Background: fatty pancreas (FP) is an old observation, but a new disease with clinical implications and several associated comorbid conditions, ranging from mild to life-threatening diseases. Herein, we aimed to assess the association between FP and pancreatic cancer (PC) development. Methods: we performed a retrospective cross-sectional study including all patients who underwent endoscopic ultrasound (EUS) for hepatobiliary indications. The study cohort was divided into patients with and without PC. Univariate and multivariate analysis were used to assess the association of several parameters with PC. Results: overall, 519 patients were included in the study. Of them, 48 had PC (PC group), and 471 did not (non-PC group). In univariate analysis, age (OR 1.04, 95% CI 1.01–1.07, p = 0.004), congestive heart failure (CHF) (OR 3.89, 95% CI 1.72–8.79, p = 0.001), ischemic heart disease (IHD) (OR 3.36, 95% CI 1.59–7.05, p = 0.001), hypertension (OR 2.42, 95% CI 1.33–4.41, p = 0.004) and fatty pancreas (FP) (OR 2.62, 95% CI 1.23–5.57, p = 0.01) were significantly associated with PC. In multivariate logistic regression analysis, only FP kept its association (OR 2.35, 95% CI 1.04–5.33, p = 0.04). Conclusion: FP was significantly associated with PC. A follow-up plan should be considered for individuals with FP.
Associations of IL-18 with Altered Cardiovascular Risk Profile in Psoriatic Arthritis and Ankylosing Spondylitis
Authors: Bonek Krzysztof , Kuca-Warnawin Ewa , Kornatka Anna , Zielińska Agnieszka , Wisłowska Małgorzata , Kontny Ewa , Głuszko Piotr
Published: January 2022

Objective: To investigate the associations of IL-18 serum levels with serum lipids, cardiovascular risk, and disease activity in patients with ankylosing spondylitis (AS) and psoriatic arthritis (PsA) with axial (axPsA) and peripheral (perPsA) joint involvement. Methods: 155 adult patients (PsA 61/AS 94) were enrolled in the study. Standard disease activity indices, BASDAI, and ASDAS, were calculated for AS and PsA and DAPSA for PsA. Sera from peripheral blood samples were obtained after night fasting. Serum concentrations of cytokines (IL-18, IL-17) were measured by ELISA, while lipid profile with total cholesterol (TC), triglycerides (TG), low-density cholesterol-(LDL), high-density cholesterol (HDL), and C-reactive protein (CRP) concentrations were determined using routine procedures. The atherogenic index was calculated using the standard formula AI = TC/HDL. Results: Patients with PsA and peripheral joint involvement (perPsA) had significantly higher IL-18 serum levels than axial PsA and AS patients (medians 160 vs. 116 vs. 80 pg/mL). In patients with PsA and in the subgroup with PsA+ ischemic heart disease (IHD), IL-18 positively correlated with atherogenic index (AI) (rho = 0.46 and rho = 0.67, respectively) and TG serum concentrations (rho = 0.4 and rho = 0.675), while negatively with HDL levels (rho = −0.37 and rho = −0.608). In PsA + IHD subgroup IL-18 serum levels correlated positively also with disease activity (DAPSA) (rho = 0.613). Importantly, in patients with perPsA, characterized by the highest IL-18 serum levels, cardiovascular risk, and frequency of both hypertriglyceridemia and IHD, positive correlations between IL-18 and IL-17 (rho = 0.47, p = 0.002), TG (rho = 0.45 p = 0.01) levels and AI (rho = 0.63 p = 0.021) were found. Whereas linear regression models revealed that IL-17, TG concentrations and the tender joint count had an impact on IL-18 Conclusions: We confirmed that patients with perPsA are characterized by a more pronounced proinflammatory and proatherogenic cardiovascular risk profile than patients with axPsA and AS. Importantly our study indicates that in PsA, but not in AS, elevated serum concentration of IL-18 is associated with higher disease activity and proatherogenic lipid profile, leading to a higher cardiovascular risk. Thus, our results point out IL-18 as a critical contributor in these pathological processes and possible therapeutic targets.
Effect of Toothpaste on the Surface Roughness of the Resin-Contained CAD/CAM Dental Materials: A Systematic Review
Authors: Di Fiore Adolfo , Stellini Edoardo , Basilicata Michele , Bollero Patrizio , Monaco Carlo
Published: January 2022

Background: The purpose of this review is to describe the possible effect of toothbrushing on surface roughness of resin-contained CAD/CAM materials. Methods: Systematic literature search for articles published in peer-reviewed journals between January 2000 and February 2020 has been conducted, which evaluated the effect of brushing on surface roughness of resin-contained CAD/CAM dental materials. The research was conducted in Scopus, PubMed/Medline, Web of Science, Embase, and Science Direct using a combination of the following MeSH/Emtree terms: “brushing”, “resin-based”, “dental”, “CAD/CAM”, and “surface roughness”. Results: A total of 249 articles were found in the search during initial screening. Fifty-five articles were selected for the full-text evaluation after the steps of reading of abstract/title and remotion of duplicate. Only six articles fulfilled the inclusion criteria. The Cohen’s Kappa agreement test showed an index of 0.91 for full-text. Discussion: Four of five selected articles identified an increase of surface roughness on resin-contained CAD/CAM materials after toothbrushing. Although all the articles examined used different toothpastes with no homogeneous relative dentine abrasivity (RDA) and cycles of brushing, the findings are about the same. The possible reason is attributable to the compositions of the resin-contained CAD/CAM materials. Conclusions: The surface roughness of most resin-contained CAD/CAM materials was affected by artificial toothbrushing. Correct knowledge of the composition of the dental material and toothpastes is fundamental to avoid an increase of surface roughness on prosthetic rehabilitation.
Transplantation of Organs from Hepatitis C Virus-Positive Donors under Direct-Acting Antiviral Regimens
Authors: Zahid Muhammad Nauman
Published: January 2022

There is a discrepancy between the patients requiring organ transplants and the donors available to meet that demand. Many patients die every year while on the waiting list, and there is a need to bridge this gap. For many years, medical practitioners have been apprehensive of using donor organs from donors who have tested positive for the Hepatitis C virus (HCV), and with good reason. HCV has been proven to be among the leading causes of liver diseases requiring liver transplants. Over the years, studies have been carried out to find a treatment for Hepatitis C. The advent of direct-acting antivirals revolutionized the medical world. These medication regimens have been proven to treat Hepatitis C in transplant patients effectively. This systematic review will examine how DAA treatments affect transplants of different organs from HCV-positive donors to HCV-negative recipients.
Head-Mounted Display-Based Augmented Reality for Image-Guided Media Delivery to the Heart: A Preliminary Investigation of Perceptual Accuracy
Authors: Doughty Mitchell , Ghugre Nilesh R.
Published: January 2022

By aligning virtual augmentations with real objects, optical see-through head-mounted display (OST-HMD)-based augmented reality (AR) can enhance user-task performance. Our goal was to compare the perceptual accuracy of several visualization paradigms involving an adjacent monitor, or the Microsoft HoloLens 2 OST-HMD, in a targeted task, as well as to assess the feasibility of displaying imaging-derived virtual models aligned with the injured porcine heart. With 10 participants, we performed a user study to quantify and compare the accuracy, speed, and subjective workload of each paradigm in the completion of a point-and-trace task that simulated surgical targeting. To demonstrate the clinical potential of our system, we assessed its use for the visualization of magnetic resonance imaging (MRI)-based anatomical models, aligned with the surgically exposed heart in a motion-arrested open-chest porcine model. Using the HoloLens 2 with alignment of the ground truth target and our display calibration method, users were able to achieve submillimeter accuracy (0.98 mm) and required 1.42 min for calibration in the point-and-trace task. In the porcine study, we observed good spatial agreement between the MRI-models and target surgical site. The use of an OST-HMD led to improved perceptual accuracy and task-completion times in a simulated targeting task.
Casein Kinase 2 Mediates Degradation of Transcription Factor Pcf1 during Appressorium Formation in the Rice Blast Fungus
Authors: Huang Pengyun , Li Yan , Wang Jing , Wang Qing , Huang Zhicheng , Liu Xiaohong , Lin Fucheng , Lu Jianping
Published: January 2022

The appressorium is a specialized structure that is differentiated from Magnaporthe oryzae spores that can infect host cells. In the process of cellular transformation from spore to appressorium, the contents inside the spores are transferred into appressoria, accompanied by major differences in the gene expression model. In this study, we reported a transcription factor (TF), Pcf1, which was depressed at the transcription level and degraded at the protein level in nuclei of incipient appressoria at four hpi (hours post inoculation). To investigate its degradation mechanism, the interacting proteins of Pcf1 were identified using an immunoprecipitation-mass spectrometry (IP-MS) assay. Yeast two-hybrid (Y2H) and co-IP (co-immunoprecipitation) assays confirmed that Pcf1 interacted with the casein kinase 2 (CK2) holoenzyme through direct combination with the CKb2 subunit. Moreover, Pcf1 was ubiquitinated in the hyphae. These changes in Pcf1 protein levels in nuclei provide a new clue of how TFs are degraded during appressorium formation: temporarily unnecessary TFs in spores are phosphorylated through interacting with CK2 enzyme and are then ubiquitinated and digested by the ubiquitin proteasome system (UPS).