bluuebunny/crossref_metadata_2025 huggingface dataset:
The Stem Sap Flow and Water Sources for Tamarix ramosissima in an Artificial Shelterbelt With a Deep Groundwater Table in Northwest China
Authors: Liu Feiyao , You Quangang , Xue Xian , Peng Fei , Huang Cuihua , Ma Shaoxiu , Pan Jing , Shi Yaofang , Chen Xiaojie
Published: March 2022

The shelterbelt forest between oases and the desert plays a vital role in preventing aeolian disasters and desertification in arid regions of northwest China.Tamarix ramosissima(T.ramosissima), a typical perennial and native xerophyte shrub in Northwest China, grows naturally and is widely used in building artificial shelterbelt forests. The balance between water consumption and the availability of water determines the survival and growth ofT.ramosissima.HowT. ramosissimacopes with extremely low rainfall and a deep groundwater table remains unknown. To answer this, the transpiration and the water sources ofT.ramosissimawere investigated by the heat balance and oxygen isotopic analysis method, respectively. Our results show that the dailyT.ramosissimastem sap flow (SSF) was positively correlated with air temperature (Ta), photosynthetically active radiation (PAR), and the vapor pressure deficit (VPD). We found no significant relationship between the daily SSF and soil moisture in shallow (0–40 cm) and middle (40–160 cm) soil layers. Oxygen isotope results showed thatT. ramosissimamainly sources (>90%) water from deep soil moisture (160–400 cm) and groundwater (910 cm). Diurnally,T.ramosissimaSSF showed a hysteresis response to variations in PAR, Ta, and VPD, which suggests that transpiration suffers increasingly from water stress with increasing PAR, Ta, and VPD. Our results indicate that PAR, Ta, and VPD are the dominant factors that controlT. ramosissimaSSF, not precipitation and shallow soil moisture. Deep soil water and groundwater are the primary sources forT.ramosissimain this extremely water-limited environment. These results provide information that is essential for proper water resource management during vegetation restoration and ecological reafforestation in water-limited regions.
Differential Investment Strategies in Leaf Economic Traits Across Climate Regions Worldwide
Authors: Ren Liang , Huang Yongmei , Pan Yingping , Xiang Xiang , Huo Jiaxuan , Meng Dehui , Wang Yuanyuan , Yu Cheng
Published: March 2022

The leaf economics spectrum (LES) is the leading theory of plant ecological strategies based on functional traits, which explains the trade-off between dry matter investment in leaf structure and the potential rate of resource return, revealing general patterns of leaf economic traits investment for different plant growth types, functional types, or biomes. Prior work has revealed the moderating role of different environmental factors on the LES, but whether the leaf trait bivariate relationships are shifted across climate regions or across continental scales requires further verification. Here we use the Köppen–Geiger climate classification, a very widely used and robust criterion, as a basis for classifying climate regions to explore climatic differences in leaf trait relationships. We compiled five leaf economic traits from a global dataset, including leaf dry matter content (LDMC), specific leaf area (SLA), photosynthesis per unit of leaf dry mass (Amass), leaf nitrogen concentration (Nmass), and leaf phosphorus concentration (Pmass). Moreover, we primarily used the standardized major axis (SMA) analysis to establish leaf trait bivariate relationships and to explore differences in trait relationships across climate regions as well as intercontinental differences within the same climate type. Leaf trait relationships were significantly correlated across almost all subgroups (P < 0.001). However, there was no common slope among different climate zones or climate types and the slopes of the groups fluctuated sharply up and down from the global estimates. The range of variation in the SMA slope of each leaf relationship was as follows: LDMC–SLA relationships (from −0.84 to −0.41); Amass–SLA relationships (from 0.83 to 1.97); Amass–Nmass relationships (from 1.33 to 2.25); Nmass–Pmass relationships (from 0.57 to 1.02). In addition, there was significant slope heterogeneity among continents within the Steppe climate (BS) or the Temperate humid climate (Cf). The shifts of leaf trait relationships in different climate regions provide evidence for environmentally driven differential plant investment in leaf economic traits. Understanding these differences helps to better calibrate various plant-climate models and reminds us that smaller-scale studies may need to be carefully compared with global studies.
An Upgraded, Highly Saturated Linkage Map of Japanese Plum (Prunus salicina Lindl.), and Identification of a New Major Locus Controlling the Flavan-3-ol Composition in Fruits
Authors: Battistoni Benjamín , Salazar Juan , Vega Wladimir , Valderrama-Soto Diego , Jiménez-Muñoz Pablo , Sepúlveda-González Ailynne , Ahumada Sebastián , Cho Isaac , Gardana Claudio Sebastiano , Morales Héctor , Peña-Neira Álvaro , Silva Herman , Maldonado Jonathan , González Mauricio , Infante Rodrigo , Pacheco Igor
Published: March 2022

Japanese plum fruits are rich in phenolic compounds, such as anthocyanins and flavan-3-ols, whose contents vary significantly among cultivars. Catechin (C) and epicatechin (EC) are flavan-3-ol monomers described in the fruits of this species and are associated with bitterness, astringency, antioxidant capacity, and susceptibility to enzymatic mesocarp browning. In this study, we aimed to identify quantitative trait loci (QTL) associated with the content of flavan-3-ol in Japanese plum fruits. We evaluated the content of C and EC in the mesocarp and exocarp of samples from 79 and 64 seedlings of an F1 progeny (<‘98–99’ × ‘Angeleno’>) in the first and second seasons, respectively. We also constructed improved versions of linkage maps from ‘98–99’ and ‘Angeleno,’ presently called single-nucleotide polymorphisms (SNPs) after mapping the already available GBS reads to Prunus salicina Lindl. cv. ‘Sanyueli’ v2.0 reference genome. These data allowed for describing a cluster of QTLs in the cultivar, ‘Angeleno,’ associated with the flavan-3-ol composition of mesocarp and exocarp, which explain up to 100% of the C/EC ratio. Additionally, we developed a C/EC metabolic marker, which was mapped between the markers with the highest log of odds (LOD) scores detected by the QTL analysis. The C/EC locus was located in the LG1, at an interval spanning 0.70 cM at 108.30–108.90 cM. Our results suggest the presence of a novel major gene controlling the preferential synthesis of C or EC in the Japanese plum fruits. This study is a significant advance in understanding the regulation of synthesizing compounds associated with fruit quality, postharvest, and human health promotion.
Genome-Wide Analysis of DREB Genes Identifies a Novel Salt Tolerance Gene in Wild Soybean (Glycine soja)
Authors: Hou Zhihong , Li Yongli , Cheng Yuhan , Li Weiwei , Li Tai , Du Hao , Kong Fanjiang , Dong Lidong , Zheng Dianfeng , Feng Naijie , Liu Baohui , Cheng Qun
Published: March 2022

Salt stress is a major factor limiting the growth and yield of soybean (Glycine max). Wild soybeans (Glycine soja) contain high allelic diversity and beneficial alleles that can be re-introduced into domesticated soybeans to improve adaption to the environment. However, very few beneficial alleles have been identified from wild soybean. Here, we demonstrate that wild soybean is more salt tolerant than cultivated soybean and examine dehydration responsive element-binding (DREB) family transcription factor genes to look for advantageous alleles that might improve drought tolerance in cultivated soybean. Our genome-wide analysis identified 103 DREB genes from the Glycine max genome. By combined RNA-sequencing and population genetics of wild, landrace, and cultivated soybean accessions, we show that the natural variation in DREB3a and DREB3b is related to differences in salt tolerance in soybean accessions. Interestingly, DREB3b, but not DREB3a, appears to have undergone artificial selection. Soybean plants carrying the wild soybean DREB3b allele (DREB3b39Del) are more salt tolerant than those containing the reference genome allele (DREB3bRef). Together, our results suggest that the loss of the DREB3b39Del allele through domestication of cultivated soybean may be associated with a reduction in salt tolerance. Our findings provide crucial information for improving salt tolerance in soybean through molecular breeding.
Residue Analysis and the Effect of Preharvest Forchlorfenuron (CPPU) Application on On-Tree Quality Maintenance of Ripe Fruit in “Feizixiao” Litchi (Litchi chinensis Sonn.)
Authors: Liu Xin-Sheng , Luo Ye-Cheng , Wang Si-Wei , Wang Hui-Cong , Harpaz-Saad Smadar , Huang Xu-Ming
Published: March 2022

Litchi is a highly perishable fruit. Ripe litchi fruit loses quality quickly as they hang on tree, giving a very short hanging life and thus harvest period. This study attempted to explore the roles of cytokinin in regulating fruit ripening and senescence of litchi and examine the possibility of applying cytokinin in “on-tree storage” of the fruit. Exogenous cytokinin, forchlorfenuron (CPPU), was applied at 20 mg L−1 7 weeks after full bloom on litchi (Litchi chinensis cv. Feizixiao) fruit clusters. Color parameters, chlorophylls, anthocyanins, fruit and fruit part weights, total soluble solutes (TSSs), soluble sugars, organic acids, non-anthocyanin flavonoids, ethanol, and also CPPU residue in fruit were traced. CPPU residue was higher but decreased faster in the pericarp than in the aril, where it maintained < 10 μg kg−1. CPPU had no significant effect on fruit weight but tended to increase pericarp weight. The treatment suppressed chlorophyll loss and anthocyanin accumulation in the pericarp, increased non-anthocyanin flavonoids in the aril, but had no significant effects on non-anthocyanin flavonoids in the pericarp and total sugar and organic acids in the aril. As the commercially ripe fruit hanged on tree, TSSs, total sugar, and sucrose decreased with ethanol and acetic acid accumulation in the aril. CPPU significantly suppressed the loss of sucrose and total sugar and the accumulation of ethanol and acetic acid in the aril and inhibited malondialdehyde accumulation in the pericarp of the overripe fruit. Soluble invertase, alcohol dehydrogenase, and pyruvate decarboxylase (PDC) activity and gene expression in the aril were downregulated by CPPU. The results suggest that cytokinin partially suppresses the ripening process in litchi and is effective to slow quality loss in the overripe fruit on tree.
Nep1-Like Proteins From the Biocontrol Agent Pythium oligandrum Enhance Plant Disease Resistance Independent of Cell Death and Reactive Oxygen Species
Authors: Yang Kun , Chen Chao , Wang Yi , Li Jialu , Dong Xiaohua , Cheng Yang , Zhang Huanxin , Zhai Ying , Ai Gan , Song Qingsong , Wang Baojian , Liu Wentao , Yin Zhiyuan , Peng Hao , Shen Danyu , Fang Song , Dou Daolong , Jing Maofeng
Published: March 2022

Microbial necrosis and ethylene-inducing peptide 1 (Nep1)-like proteins (NLPs) act as cytolytic toxins and immunogenic patterns in plants. Our previous work shows that cytolytic NLPs (i.e., PyolNLP5 and PyolNLP7) from the biocontrol agent Pythium oligandrum enhance plant resistance against Phytophthora pathogens by inducing the expression of plant defensins. However, the relevance between PyolNLP-induced necrosis and plant resistance activation is still unclear. Here, we find that the necrosis-inducing activity of PyolNLP5 requires amino acid residues D127 and E129 within the conserved “GHRHDLE” motif. However, PyolNLP5-mediated plant disease resistance is irrelevant to its necrosis-inducing activity and the accumulation of reactive oxygen species (ROS). Furthermore, we reveal the positive role of non-cytotoxic PyolNLPs in enhancing plant resistance against Phytophthora pathogens and the fugal pathogen Sclerotinia sclerotiorum. Similarly, non-cytotoxic PyolNLPs also activate plant defense in a cell death-independent manner and induce defensin expression. The functions of non-cytotoxic PyolNLP13/14 rely on their conserved nlp24-like peptide pattern. Synthetic Pyolnlp24s derived from both cytotoxic and non-cytotoxic PyolNLPs can induce plant defensin expression. Unlike classic nlp24, Pyolnlp24s lack the ability of inducing ROS burst in plants with the presence of Arabidopsis nlp24 receptor RLP23. Taken together, our work demonstrates that PyolNLPs enhance plant resistance in an RLP23-independent manner, which requires the conserved nlp24-like peptide pattern but is uncoupled with ROS burst and cell death.
Phenotypic and Methylome Responses to Salt Stress in Arabidopsis thaliana Natural Accessions
Authors: Lin Xiaohe , Zhou Ming , Yao Jing , Li Qingshun Q. , Zhang Yuan-Ye
Published: March 2022

Salt stress threatens plant growth, development and crop yields, and has become a critical global environmental issue. Increasing evidence has suggested that the epigenetic mechanism such as DNA methylation can mediate plant response to salt stress through transcriptional regulation and transposable element (TE) silencing. However, studies exploring genome-wide methylation dynamics under salt stress remain limited, in particular, for studies on multiple genotypes. Here, we adopted four natural accessions of the model species Arabidopsis thaliana and investigated the phenotypic and genome-wide methylation responses to salt stress through whole-genome bisulfite sequencing (WGBS). We found that salt stress significantly changed plant phenotypes, including plant height, rosette diameter, fruit number, and aboveground biomass, and the change in biomass tended to depend on accessions. Methylation analysis revealed that genome-wide methylation patterns depended primarily on accessions, and salt stress caused significant methylation changes in ∼ 0.1% cytosines over the genomes. About 33.5% of these salt-induced differential methylated cytosines (DMCs) were located to transposable elements (TEs). These salt-induced DMCs were mainly hypermethylated and accession-specific. TEs annotated to have DMCs (DMC-TEs) across accessions were found mostly belonged to the superfamily of Gypsy, a type II transposon, indicating a convergent DMC dynamic on TEs across different genetic backgrounds. Moreover, 8.0% of salt-induced DMCs were located in gene bodies and their proximal regulatory regions. These DMCs were also accession-specific, and genes annotated to have DMCs (DMC-genes) appeared to be more accession-specific than DMC-TEs. Intriguingly, both accession-specific DMC-genes and DMC-genes shared by multiple accessions were enriched in similar functions, including methylation, gene silencing, chemical homeostasis, polysaccharide catabolic process, and pathways relating to shifts between vegetative growth and reproduction. These results indicate that, across different genetic backgrounds, methylation changes may have convergent functions in post-transcriptional, physiological, and phenotypic modulation under salt stress. These convergent methylation dynamics across accession may be autonomous from genetic variation or due to convergent genetic changes, which requires further exploration. Our study provides a more comprehensive picture of genome-wide methylation dynamics under salt stress, and highlights the importance of exploring stress response mechanisms from diverse genetic backgrounds.
Comparative Transcriptome Analysis Unravels Defense Pathways of Fraxinus velutina Torr Against Salt Stress
Authors: Ma Xinmei , Liu Jian Ning , Yan Liping , Liang Qiang , Fang Hongcheng , Wang Changxi , Dong Yuhui , Chai Zejia , Zhou Rui , Bao Yan , Hou Wenrui , Yang Ke Qiang , Wu Dejun
Published: March 2022

Fraxinus velutina Torr with high salt tolerance has been widely grown in saline lands in the Yellow River Delta, China. However, the salt-tolerant mechanisms of F. velutina remain largely elusive. Here, we identified two contrasting cutting clones of F. velutina, R7 (salt-tolerant), and S4 (salt-sensitive) by measuring chlorophyll fluorescence characteristics (Fv/Fm ratio) in the excised leaves and physiological indexes in roots or leaves under salt treatment. To further explore the salt resistance mechanisms, we compared the transcriptomes of R7 and S4 from leaf and root tissues exposed to salt stress. The results showed that when the excised leaves of S4 and R7 were, respectively, exposed to 250 mM NaCl for 48 h, Fv/Fm ratio decreased significantly in S4 compared with R7, confirming that R7 is more tolerant to salt stress. Comparative transcriptome analysis showed that salt stress induced the significant upregulation of stress-responsive genes in R7, making important contributions to the high salt tolerance. Specifically, in the R7 leaves, salt stress markedly upregulated key genes involved in plant hormone signaling and mitogen-activated protein kinase signaling pathways; in the R7 roots, salt stress induced the upregulation of main genes involved in proline biosynthesis and starch and sucrose metabolism. In addition, 12 genes encoding antioxidant enzyme peroxidase were all significantly upregulated in both leaves and roots. Collectively, our findings revealed the crucial defense pathways underlying high salt tolerance of R7 through significant upregulation of some key genes involving metabolism and hub signaling pathways, thus providing novel insights into salt-tolerant F. velutina breeding.
The Rice Aspartyl-tRNA Synthetase YLC3 Regulates Amino Acid Homeostasis and Chloroplast Development Under Low Temperature
Authors: Liu Hongjia , Gong Xue , Deng Hui , Tan Jinjuan , Sun Yanqing , Wang Fang , Wu Wenjuan , Zhou Zhongjing , Xu Rumeng , He Haiyan , Lo Clive
Published: March 2022

Aminoacyl tRNA synthetases primarily function to attach specific amino acids to the corresponding tRNAs during protein translation. However, their roles in regulating plant growth and development still remain elusive. Here we reported a rice thermo-sensitive mutant yellow leaf chlorosis3 (ylc3) with reduced chlorophyll content, altered thylakoid structure, and substantially elevated levels of free aspartate, asparagine and glutamine in leaves under low temperature condition. Map-based cloning identified that YLC3 encodes an aspartyl-tRNA synthetase which is localized in cytosol and mitochondria. In addition, quantitative proteomics analysis revealed that both nuclear and chloroplast-encoded thylakoid proteins were significantly down-regulated in the mutant. On the other hand, proteins involved in amino acid metabolism and the process of protein synthesis were up-regulated in ylc3, particularly for key enzymes that convert aspartate to asparagine. Moreover, uncharged tRNA-Asp accumulation and phosphorylation of the translation initiation factor eIF2α was detected in the mutant, suggesting that YLC3 regulates the homeostasis of amino acid metabolism and chloroplast thylakoid development through modulation of processes during protein synthesis.
RNAi of Sterol Δ24-Isomerase Implicated Its Involvement in Physalin Biosynthesis in Physalis angulata L.
Authors: Yang Jiao , Tian Jingyi , Yang Yuhui , Zhu Yaru , Li Changfu , Zhang Yansheng
Published: March 2022

Physalis angulata is a renowned traditional Chinese medicine for the treatment of various conditions. Physalin is the major type of bioactive constituents conferring medicinal properties of P. angulata. Despite the medicinal importance, the pathways leading to physalin are largely unknown. In this study, we employed a transcriptomic approach to identify a Pa24ISO gene from P. angulata. Through heterologous expression in yeast, Pa24ISO was revealed to catalyze an isomerization reaction in converting 24-methylenecholesterol to 24-methyldesmosterol. Real-time PCR analysis showed that the abundance of Pa24ISO transcripts correlated with the accumulation pattern of physalin B in different tissues of P. angulata. A direct role of Pa24ISO in channeling of 24-methylenecholesterol for physalin B biosynthesis was illustrated by suppressing the gene in P. angulata via the VIGS approach. Down-regulation of Pa24ISO led to reduced levels of 24-methyldesmosterol and physalin B, accompanied with an increase of campesterol content in P. angulata. The results supported that 24ISO is involved in physalin biosynthesis in plants.
A Hierarchical Integrated Model of Self-Regulation
Authors: Blair Clancy , Ku Seulki
Published: March 2022

We present a hierarchical integrated model of self-regulation in which executive function is the cognitive component of the model, together with emotional, behavioral, physiological, and genetic components. These five components in the model are reciprocally and recursively related. The model is supported by empirical evidence, primarily from a single longitudinal study with good measurement at each level of the model. We also find that the model is consistent with current thinking on related topics such as cybernetic theory, the theory of allostasis and allostatic load, and the theory of skill development in harsh and unpredictable environments, referred to as “hidden talents.” Next, we present literature that the integrative processes are susceptible to environmental adversity, poverty-related risk in particular, while positive social interactions with caregivers (e.g., maternal sensitivity) would promote self-regulatory processes or mitigate the adverse effect of early risk on the processes. A hierarchical integrative model of self-regulation advances our understanding of self-regulatory processes. Future research may consider broader social contexts of the integrative self-regulation system, such as neighborhood/community contexts and structural racism. This can be an integral step to provide children with equitable opportunities to thrive, even among children living in socioeconomically and psychosocially disadvantaged environments.
Peer Victimization Influences Attention Processing Beyond the Effects of Childhood Maltreatment by Caregivers
Authors: Iffland Benjamin , Neuner Frank
Published: March 2022

Background

Different types of maltreatment (emotional, physical, and sexual) lead to distortions in emotion and attention processing. The present study investigated whether the experience of peer victimization in childhood and adolescence has an additional influence on attention processing in adulthood.

Methods

Two non-clinical samples consisting of individuals with different levels of experiences of maltreatment were recruited. In an evaluative conditioning task, images of faces with neutral emotional expression were either associated with short videos of intense negative statements, or associated with neutral videos. Subsequently, these faces were used as stimuli in an emotional Stroop task as well as a dot-probe task.

Results

In both tasks, hierarchical regression analyses revealed that retrospective reports of relational peer victimization made an incremental contribution to the prediction of attentional biases beyond child maltreatment. In the emotional Stroop task, emotional abuse was the strongest predictor for an attentional bias showing delayed responses to negatively associated faces, while peer victimization was associated with faster responses to negatively associated faces. In the dot-probe task, relational peer victimization was the strongest predictor for an attentional bias. When the attentional bias was examined in more detail, though, peer victimization did not show incremental contributions although emotional abuse remained the strongest predictor for facilitated attention toward negatively associated neutral faces.

Conclusion

Experiences of peer victimization leave additional cognitive scars beyond effects of childhood maltreatment by caregivers. It is likely that attentional biases in the aftermath of victimization put individuals at risk for the development of psychopathology.
Virtual Reality as a Moderator of Psychedelic-Assisted Psychotherapy
Authors: Sekula Agnieszka D. , Downey Luke , Puspanathan Prashanth
Published: March 2022

Psychotherapy with the use of psychedelic substances, including psilocybin, lysergic acid diethylamide (LSD), ketamine, and 3,4-methylenedioxymethamphetamine (MDMA), has demonstrated promise in treatment of post-traumatic stress disorder (PTSD), anxiety, addiction, and treatment-resistant depression. Psychedelic-assisted psychotherapy (PP) represents a unique psychopharmacological model that leverages the profound effects of the psychedelic experience. That experience is characterized by strong dependency on two key factors: participant mindset and the therapeutic environment. As such, therapeutic models that utilize psychedelics reflect the need for careful design that promotes an open, flexible, trusting mindset and a supportive setting. To meet this need, the PP model is increasingly supplemented by auxiliary methods, including meditation, relaxation, visualization or spiritual practices. We suggest virtual reality (VR) as a full-spectrum tool able to capitalize on and catalyze the innately therapeutic aspects of the psychedelic experience, such as detachment from familiar reality, alteration of self-experience, augmentation of sensory perception and induction of mystical-type experiences. This is facilitated by VR’s evidenced capacity to: aid relaxation and reduce anxiety; buffer from external stimuli; promote a mindful presence; train the mind to achieve altered states of consciousness (ASC); evoke mystical states; enhance therapeutic alliance and encourage self-efficacy. While these unique VR features appear promising, VR’s potential role in PP remains speculative due to lack of empirical evidence on the combined use of VR and PP. Given the increased commercial interest in this synergy there is an urgent need to evaluate this approach. We suggest specific VR models and their role within PP protocols to inspire future direction in scientific research, and provide a list of potential disadvantages, side effects and limitations that need to be carefully considered. These include sensory overstimulation, cyber-sickness, triggering memories of past traumatic events as well as distracting from the inner experience or strongly influencing its contents. A balanced, evidence-based approach may provide continuity across all phases of treatment, support transition into and out of an ASC, deepen acute ASC experiences including mystical states and enrich the psychotherapeutic process of integration. We conclude that the potential application of VR in modulating psychedelic-assisted psychotherapy demands further exploration and an evidence-based approach to both design and implementation.
Tracking Object-State Representations During Real-Time Language Comprehension by Native and Non-native Speakers of English
Authors: Kang Xin , Ge Haoyan
Published: March 2022

The present “visual world” eye-tracking study examined the time-course of how native and non-native speakers keep track of implied object-state representations during real-time language processing. Fifty-two native speakers of English and 46 non-native speakers with advanced English proficiency joined this study. They heard short stories describing a target object (e.g., an onion) either having undergone a substantial change-of-state (e.g., chop the onion) or a minimal change-of-state (e.g., weigh the onion) while their eye movements toward competing object-states (e.g., a chopped onion vs. an intact onion) and two unrelated distractors were tracked. We found that both groups successfully directed their visual attention toward the end-state of the target object that was implied in the linguistic context. However, neither group showed anticipatory eye movements toward the implied object-state when hearing the critical verb (e.g., “weigh/chop”). Only native English speakers but not non-native speakers showed a bias in visual attention during the determiner (“the”) before the noun (e.g., “onion”). Our results suggested that although native and non-native speakers of English largely overlapped in their time-courses of keeping track of object-state representations during real-time language comprehension, non-native speakers showed a short delay in updating the implied object-state representations.
Developing Future-Ready University Graduates: Nurturing Wellbeing and Life Skills as Well as Academic Talent
Authors: Gan Tzyy Yang , Beevi Zuhrah , Low Jasmine , Lee Peter J. , Hall Deborah Ann
Published: March 2022

Higher education is starting to embrace its role in promoting student wellbeing and life skills, especially given the concerning levels of poor mental health and uncertainties in the future job market. Yet, many of the published studies evaluating positive educational teaching methods thus far are limited to interventions delivered to small student cohorts and/or imbedded within elective wellbeing courses, and are focussed on developed Western countries. This study addressed this gap by investigating the effectiveness of an institution-wide compulsory course informed by the principles of Seligman’s Wellbeing Theory. The course was delivered at a British university in a developing country in Southeast Asia. It purposefully sought to nurture growth-oriented outcomes (including self-awareness, positive emotions, and personal effectiveness) and was taken by an entire cohort of year one undergraduate students. We tested the effectiveness of the curriculum content and staff coaching style in achieving life skills, and evaluated how these perceptions influenced students’ subjective wellbeing. A convergent mixed-methods design was used with 350 survey respondents and 11 interviewees. Perceived life skills scores showed a 2.5% improvement at the end of the course. Partial Least Squares Structural Equation Modelling tested the predicted relationships between variables. All relationships were statistically significant, but the influence of course design and educators’ style on life skills acquisition (50.8% of the variance) was moderate, while the effect on subjective happiness and life satisfaction (4–5% of the variance) was very weak. Qualitative data indicated that while quantifiable benefits to wellbeing might not be immediate, students did anticipate longer-term benefits for happiness and life satisfaction. This finding suggests that such a novel educational approach is well-received by Asian students and may sow the seeds for future benefit by positively impacting on their skills, behaviours, attitudes, and values. To achieve optimal flourishing at university, we recommend exploring teaching practises that combine positive education with coaching psychology practises.
The Relationship Between Self-Care Behavior and Concerns About Body Image in Patients Undergoing Hemodialysis in Iran
Authors: Sharif Nia Hamid , Kohestani Daniyal , Froelicher Erika Sivarajan , Ibrahim Fatima Muhammad , Ibrahim Maryam Mohammad , Bayat Shahparast Fatemeh , Goudarzian Amir Hossein
Published: March 2022

Background and Aim

Hemodialysis treatment saves the life of people with end-stage renal disease (ERDS), but does not prevent the suffering of the disease, anxiety, hopelessness, and so on. Many studies have been performed on self-care behaviors as well as body image in different patients, but so far, no research has been done to investigate the relationship between self-care behavior and body image concerns in hemodialysis patients. Therefore, the aims of this study are to determine the relationship between self-care behavior and body image concern in patients undergoing hemodialysis.

Materials and Methods

A cross-sectional design was used to evaluate Self-care Behavior and Concerns about Body Image in a convenience sample of 280 patients with ERDS. Measures included demographic factors, health characteristics, and validated instruments of the study constructs Body Image Concern Questionnaire (BICI) and Assessment of self-care behaviors with arteriovenous fistula (ASBHD-AVF).

Results

This study showed that self-care behaviors have the ability to predict body image concerns. There was a negative and significant relationship between self-care behavior and body image concern (B = −0.162, β = −0.140, p = 0.020). These variables explain 7.5% of predictors.

Conclusion

Although the findings of the present study showed that increasing age and improving the level of self-care behaviors in patients undergoing hemodialysis reduces body image anxiety, but women were the strongest predictor of body image anxiety.
Financing Costs and Health Effects of Air Pollution in the Tri-City Agglomeration
Authors: Czechowski Piotr O. , Piksa Konstancja , Da̧browiecki Piotr , Oniszczuk-Jastrząbek Aneta I. , Czermański Ernest , Owczarek Tomasz , Badyda Artur J. , Cirella Giuseppe T.
Published: March 2022

This paper examines the relationship between the presence of air pollution and incidence of selected respiratory diseases in the urban population of the Tri-City agglomeration. The study takes into consideration the specific character of the region, relating to coastal, and port-based shipping. Three research hypotheses formulated the study. General regression models were used to identify the health effects of air pollution and developed health costs were calculated in relation to the treatment of diseases. The findings have shown that air pollution and climatic conditions in the Tri-City aggravate the symptoms of bronchial asthma, while also increasing the number of cases of exacerbated chronic obstructive pulmonary disease and pneumonia. The evidence demonstrates the negative impact of shipping on the health condition of the inhabitants. The calculations have shown the extent of financial losses incurred in connection with the treatment of diseases found to have been caused by air pollution. The estimated health costs turned out to be significant for each of the examined diseases. The financial inefficiency of the Polish health care system has also been demonstrated. All the models have been identified for monthly data for the first time.
Prediction of Atrial Fibrillation in Hospitalized Elderly Patients With Coronary Heart Disease and Type 2 Diabetes Mellitus Using Machine Learning: A Multicenter Retrospective Study
Authors: Xu Qian , Peng Yan , Tan Juntao , Zhao Wenlong , Yang Meijie , Tian Jie
Published: March 2022

Background

The objective of this study was to use machine learning algorithms to construct predictive models for atrial fibrillation (AF) in elderly patients with coronary heart disease (CHD) and type 2 diabetes mellitus (T2DM).

Methods

The diagnosis and treatment data of elderly patients with CHD and T2DM, who were treated in four tertiary hospitals in Chongqing, China from 2015 to 2021, were collected. Five machine learning algorithms: logistic regression, logistic regression+least absolute shrinkage and selection operator, classified regression tree (CART), random forest (RF) and extreme gradient lifting (XGBoost) were used to construct the prediction models. The area under the receiver operating characteristic curve (AUC), sensitivity, specificity, and accuracy were used as the comparison measures between different models.

Results

A total of 3,858 elderly patients with CHD and T2DM were included. In the internal validation cohort, XGBoost had the highest AUC (0.743) and sensitivity (0.833), and RF had the highest specificity (0.753) and accuracy (0.735). In the external verification, RF had the highest AUC (0.726) and sensitivity (0.686), and CART had the highest specificity (0.925) and accuracy (0.841). Total bilirubin, triglycerides and uric acid were the three most important predictors of AF.

Conclusion

The risk prediction models of AF in elderly patients with CHD and T2DM based on machine learning algorithms had high diagnostic value. The prediction models constructed by RF and XGBoost were more effective. The results of this study can provide reference for the clinical prevention and treatment of AF.
Urine Phthalate Levels and Liver Function in US Adolescents: Analyses of NHANES 2007–2016
Authors: Xiang Shiting , Dong Jie , Li Xun , Li Chao
Published: March 2022

Background

Phthalates are non-persistent chemicals with endocrine-disrupting abilities widely used in a variety of consumer products. Evidence for the effects of phthalate exposure on liver function in adolescents is lacking.

Methods

Data were analyzed from the combined 2007–2016 National Health and Nutrition Examination Survey (NHANES). Ultimately, a total of 1,650 adolescents aged 12–19 years were selected as the samples. Weighted linear regression was used to investigate the effects of urinary phthalate metabolites on liver function indexes.

Results

Weighted Linear regression models showed that MCOP was negatively associated with TBIL (β = −0.0435, PFDR = 0.007), ΣDEHP (β = −0.0453, PFDR = 0.003) and MCOP (β = −0.0379, PFDR = 0.006) were negatively correlated with ALB, while MCPP was positively correlated with ALB (β = 0.0339, PFDR = 0.024), and MCOP was negatively correlated with TP (β = −0.0551; PFDR = 0.004).

Conclusions

Phthalate metabolites were significantly but weakly associated with changes in liver function indicators among US adolescents. Future work should further examine these relationships.
Re-Examining the Financial Structure and Health Nexus in Asian Economies
Authors: Xu Yanling , Tao Ran , Mahmood Chaudhry Kashif , Altuntaş Mehmet
Published: March 2022

The study's main purpose is to estimate the impact of the financial structure of Asian economies on the healthcare sector from 2000 to 2019. For empirical estimation, we relied on two-stage least square (2SLS) and generalized method of moment (GMM) estimation techniques. Two different proxies, infant mortality and life expectancy, were used in the analysis to represent the health status of the people. The findings of both 2SLS and GMM models confirm that improved financial structure causes life expectancy to rise and infant mortality to fall. Moreover, the increased usage of the internet also exerts a positive impact on the health status of Asians. Further, the rise in gross domestic product (GDP) and health expenditures also improve the health status of Asians by increasing their life expectancy and reducing their infant mortality rate. Improvement in financial structure causes the health status of the people to rise. Therefore, to achieve superior health status, the development of financial structure should be part and parcel of health policies and strategies in Asian economies.
Single Center Retrospective Review of Post-laparotomy CT Abdomen and Pelvis Findings and Trends
Authors: Steffey Dylan C. , Chishti Emad A. , Acevedo Maximo J. , Acosta Luis F. , Lee James T.
Published: March 2022

Purpose

To identify common findings visualized on CT following damage control laparotomy, including post-surgical changes and additional injuries, and to determine change in frequency of post-laparotomy CT at our institution over time.

Methods

Single institution, IRB-Exempt, retrospective review of the University of Kentucky trauma registry from 1/2006 to 2/2019 for all trauma patients undergoing exploratory laparotomy initially and subsequently undergoing CT of the abdomen and pelvis within 24 hours. Operative findings from surgical operation notes and findings reported on post-laparotomy CT were recorded, including vascular and solid organ injuries, operative changes, free intraperitoneal fluid/air, and retroperitoneal findings. Next steps in management were also recorded.

Results

In total 1,047 patients underwent exploratory laparotomy initially at our institution between 1/2006-2/2019. Of those, only 96 had a diagnostic CT of the abdomen and pelvis within 24 h after initial surgery, first occurring in 2010. Among these 96, there were 71 blunt and 25 penetrating injuries. Most common injuries recognized during exploratory laparotomy were bowel/mesentery (55), spleen (34), and liver (26). Regarding CT findings, all patients (96/96, 100%) had residual pneumoperitoneum, 84/96 (87.5%) had residual hemoperitoneum, 36/96 (37.5%) noted post-surgical changes or additional injuries to the spleen, 36/96 (37.5%) to the bowel/mesentery, and 32/96 (33.3%) to the liver, and 34/96 (35.4%) were noted to have pelvic fractures. After CT, 31/96 (32.3%) went back to the OR for relook laparotomy and additional surgical intervention and 7/96 (7.3%) went to IR for embolization. Most common procedures during relaparotomy involved the bowel (8) and solid organs (6).

Conclusions

CT examination within 24 h post damage control laparotomy was exceedingly rare at our institution prior to 2012 but has steadily increased. Frequency now averages 20.5% yearly. Damage control laparotomy is an uncommon clinical scenario; however, knowledge of frequent injuries and common post-operative changes will aid in radiologist detection of additional injuries helping shape next step management and provide adequate therapy.
How Is Mass Spectrometry Tackling the COVID-19 Pandemic?
Authors: Ibáñez Alfredo J.
Published: March 2022

Most of us have never faced a pandemic before. The World Health Organization declared the 2019 novel coronavirus infectious disease (COVID-19), caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2 virus), a pandemic by March 11th, 2020. Today, this illness has reported more than 5′331,019 fatalities worldwide (December 17th, 2021). The COVID-19 pandemic has posed an unprecedented global challenge and put the academic community on “the spot.” The following mini-review reports how the MS community improved the understanding of the SARS-CoV-2 virus pathophysiology while developing diagnostic procedures to complement the PCR-based approaches. For example, MS researchers identified the interaction sites between the SARS-CoV-2 virus and their hosts; this new knowledge is critical for developing antiviral drugs. MS researchers also realized that COVID-19 should be considered a systemic disease and not just a respiratory illness since its metabolic, lipidomic, and proteomic profile reflects four different clinical disorders: 1) acute inflammatory response, 2) a cardiovascular disease, 3) a prediabetic/diabetes and 4) liver dysfunction. Furthermore, MS researchers put forth the knowledge that the metabolic and lipidomic profile of several patients remained altered after being discharged, thus hinting at the scientific basis for the long COVID syndrome.
Fatty Acids, Amphiregulin Production, and Lung Function in a Cohort of Midwestern Veterans
Authors: Hanson Corrine , Ponce Jana , Isaak Mia , Heires Art , Nordgren Tara , Wichman Chris , Furtado Jeremy D. , LeVan Tricia , Romberger Debra
Published: March 2022

Rationale

The relationship between many fatty acids and respiratory outcomes remains unclear, especially with regard to mechanistic actions. Altered regulation of the process of lung repair is a key feature of chronic lung disease and may impact the potential for pulmonary rehabilitation, but underlying mechanisms of lung repair following injury or inflammation are not well-studied. The epidermal growth factor receptor agonist amphiregulin (AREG) has been demonstrated to promote lung repair following occupational dust exposure in animals. Studies suggest the polyunsaturated fatty acid (PUFA) docosahexaenoic acid (DHA) may enhance the production of AREG. The objective of this study was to determine the relationship between fatty acids and lung function in a population of veterans and determine if fatty acid status is associated with concentrations of AREG.

Materials and Methods

Data were collected from a cross-sectional study of veterans within the Nebraska-Western Iowa Health Care System. Whole blood assays were performed to quantify AREG concentrations via a commercially available ELISA kit. Fatty acids from plasma samples from the same patients were measured using gas-liquid chromatography. Intakes of fatty acids were quantified with a validated food frequency questionnaire. Linear regression models were used to determine whether plasma fatty acids or intakes of fatty acids predicted lung function or AREG concentrations. A p < 0.05 was considered statistically significant.

Results

Ninety participants were included in this analysis. In fully adjusted models, plasma fatty acids were associated with AREG production, including the PUFA eicosapentaenoic acid (EPA) (β = 0.33, p = 0.03) and the monounsaturated fatty acid octadecenoic acid: (β = −0.56, p = 0.02). The omega-3 PUFA docosapentaenoic acid (DPA) was positively associated with lung function (β = 0.28, p = 0.01; β = 26.5, p = 0.05 for FEV1/FVC ratio and FEV1 % predicted, respectively), as were the omega-6 PUFAs eicosadienoic acid (β = 1.13, p < 0.001; β = 91.2, p = 0.005 for FEV1/FVC ratio and FEV1 % predicted, respectively) and docosadienoic acid (β = 0.29, p = 0.01 for FEV1/FVC ratio). Plasma monounsaturated and saturated fatty acids were inversely associated with lung function.

Conclusion

Opposing anti- and pro-inflammatory properties of different fatty acids may be associated with lung function in this population, in part by regulating AREG induction.
Transparent Interaction Based Learning for Human-Robot Collaboration
Authors: Bagheri Elahe , De Winter Joris , Vanderborght Bram
Published: March 2022

The number of collaborative robots that perform different tasks in close proximity to humans is increasing. Previous studies showed that enabling non-expert users to program a cobot reduces the cost of robot maintenance and reprogramming. Since this approach is based on an interaction between the cobot and human partners, in this study, we investigate whether making this interaction more transparent can improve the interaction and lead to better performance for non-expert users. To evaluate the proposed methodology, an experiment with 67 participants is conducted. The obtained results show that providing explanation leads to higher performance, in terms of efficiency and efficacy, i.e., the number of times the task is completed without teaching a wrong instruction to the cobot is two times higher when explanations are provided. In addition, providing explanation also increases users’ satisfaction and trust in working with the cobot.
LatitudeTM Oil as a Sustainable Alternative to Dietary Fish Oil in Rainbow Trout (Oncorhynchus mykiss): Effects on Filet Fatty Acid Profiles, Intestinal Histology, and Plasma Biochemistry
Authors: Hong Jeongwhui , Bledsoe Jacob W. , Overturf Kenneth E. , Lee Seunghan , Iassonova Diliara , Small Brian C.
Published: March 2022

The aim of this study was to evaluate the effects of Latitude™ oil (transgenic canola) fed to rainbow trout, Oncorhynchus mykiss, for 52 weeks on growth performance, non-specific immune responses, histology, and filet omega-3 fatty acid content. Latitude™ oil (LO) has high lipid digestibility (93%), and contains omega-3 fatty acids eicosapentaenoic acid (EPA, C20:5n-3), docosapentaenoic acid (DPA, C22:5n-3), and docosahexaenoic acid (DHA, C22:6n-3). Three isonitrogenous (49%), isolipidic (20%) and isocaloric (24.2 MJ kg−1) diets differing by lipid source (0, 8, or 16% LO, replacing fish oil and poultry fat) were fed over an entire production cycle beginning with 19 g juvenile fish. At the end of the 52-week feeding trial, final body weight, weight gain and specific growth rate of fish fed 8% LO (LO-8) and 16% LO (LO-16) diets were significantly higher than those fed the 0% LO (LO-0) diet (P < 0.05). Phagocytic respiratory burst in fish fed the LO-16 diet was significantly higher than those fish fed the other 2 diets (P < 0.05). There were no differences in superoxide dismutase, catalase and lysozyme. Histological examination of the distal intestine indicated reduced inflammation in fish fed the LO-8 diet but not the LO-0 and LO-16 diets. Filet DHA content of fish fed the LO-8 and LO-16 diets were similar to those of fish fed the LO-0 diet. As these diets had lower DHA content, this suggests dietary EPA and DPA from LO was converted to DHA and deposited in the filet. This is supported by increased expression of genes involved in fatty acid elongation, desaturation and beta oxidation in both liver and muscle of fish fed LO (P < 0.05). Total EPA+DHA content of the edible filet ranged between 1,079–1,241 mg 100 g−1 across treatments, each providing the recommended daily intake for human consumption (500–1,000 mg day−1). Overall, this study demonstrated that LO fed over an entire production period is a highly digestible lipid source suitable and sustainable for meeting the fatty acid requirements of rainbow trout, as well as consumer expectations for filet omega-3 fatty acid content.