bluuebunny/crossref_metadata_2025 huggingface dataset:
Case Report: Clinicopathological characteristics of patients with gastric cancer with features of a submucosal tumour
Authors: Wang Chunnian , Ye Fusang , Zhang Huan , Chen Jie , Meng Lingli , He Xianglei
Published: March 2023

Purpose

To investigate the clinicopathological characteristics, diagnosis and key points in the differential diagnosis of patients with gastric cancer (GC) with features of a submucosal tumour (GCSMT).

Methods

The clinical presentation and imaging findings of four GCSMT cases diagnosed at our centre from 2016 to 2021 were observed and their clinicopathological outcomes were analysed. The related literature was reviewed. Based on our collected data and the related literature, a total of 31 cases of GCSMT can be summarized.

Results

22 out of 31 cases did not present obvious symptoms and were accidentally discovered during gastroscopic examination. Only 10 patients experienced symptoms such as gastric discomfort, upper abdominal swelling and pain, haematemesis, or haematochezia. The male to female ratio was 22:9 and the age of onset ranged from 40 to 81 years (median age: 63 years). Tumours were located in the upper and middle third of the stomach (24/31), and in the lower third(7/31). The tumour diameter ranged from 0.6 to 7.3 cm, with an average value of 2.5 cm. Endoscopically, the disease manifested as SMTs, with the gastric mucosal surface appearing normal. Most patients underwent radical gastrectomy for GC (80.6%, 25/31). The pathological diagnoses of the 31 cases of GCSMT included well- and moderately-differentiated adenocarcinoma (6/31), poorly differentiated adenocarcinoma or signet ring cell carcinoma 6/31), mucinous adenocarcinoma (9/31), lymphoepithelioma-like carcinoma (7/31), gastric adenocarcinoma of the fundic gland type (3/31). Stage T1b and T2 tumours accounted for 56.7% (17/30) and 26.7% (8/30) of all cases. Lymph node metastases were found in six cases (20.0%, 6/30), whereas distant metastasis was not observed in any of the cases. For the 16 patients whose follow-up data were available, the follow-up time was 5–66 months, during which recurrence or metastasis was not observed.

Conclusion

GCSMT is a rare disease that is often difficult to accurately diagnose through endoscopic biopsy. The importance of gaining an understanding of this disease lies in differentiating it from other SMTs (mostly mesenchymal tumours) to avoid misdiagnosis and missed diagnosis and enable the early diagnosis and treatment of patients.
Clinicopathological features and prognostic outcomes of molecularly defined entities in the new edition of the WHO classification of sinonasal carcinoma
Authors: Vuong Huy Gia , Le Thoa , Le Trang T.B. , Le Hieu Trong , El-Rassi Edward T. , McKinney Kibwei A. , Dunn Ian F.
Published: March 2023

Introduction

We investigated the clinicopathological features and prognoses of the new molecularly defined entities in latest edition of the World Health Organization (WHO) classification of sinonasal carcinoma (SNC)

Methods

Integrated data were combined into an individual patient data (IPD) meta-analysis.

Results

We included 61 studies with 278 SNCs including 25 IDH2-mutant, 41 NUT carcinoma, 187 SWI/SNF loss, and 25 triple negative SNCs (without IDH2 mutation, NUTM1 rearrangement, and SWI/SNF inactivation) for analyses. Compared to other molecular groups, NUT carcinoma was associated with a younger age at presentation and an inferior disease-specific survival. Among SNCs with SWI/SNF inactivation, SMARCB1-deficient tumors presented later in life and were associated with a higher rate of radiotherapy administration. SMARCA4-deficiency was mostly found in teratocarcinosarcoma while SMARCB1-deficient tumors were associated with undifferentiated carcinoma and non-keratinizing squamous cell carcinoma.

Conclusion

Our study facilitates our current understanding of this developing molecular-defined spectrum of tumors and their prognoses.
Molecular mechanisms of TGFβ-mediated EMT of retinal pigment epithelium in subretinal fibrosis of age-related macular degeneration
Authors: Higashijima Fumiaki , Hasegawa Mina , Yoshimoto Takuya , Kobayashi Yuka , Wakuta Makiko , Kimura Kazuhiro
Published: March 2023

Age-related macular degeneration (AMD) is one of the leading causes of blindness in the elderly, affecting the macula of the retina and resulting in vision loss. There are two types of AMD, wet and dry, both of which cause visual impairment. Wet AMD is called neovascular AMD (nAMD) and is characterized by the formation of choroidal neovascular vessels (CNVs) in the macula. nAMD can be treated with intravitreal injections of vascular endothelial growth factor (VEGF) inhibitors, which help improve vision. However, approximately half the patients do not achieve satisfactory results. Subretinal fibrosis often develops late in nAMD, leading to irreversible photoreceptor degeneration and contributing to visual loss. Currently, no treatment exists for subretinal fibrosis, and the molecular mechanisms of fibrous tissue formation following neovascular lesions remain unclear. In this review, we describe the clinical features and molecular mechanisms of macular fibrosis secondary to nAMD. Myofibroblasts play an essential role in the development of fibrosis. This review summarizes the latest findings on the clinical features and cellular and molecular mechanisms of the pathogenesis of subretinal fibrosis in nAMD and discusses the potential therapeutic strategies to control subretinal fibrosis in the future.
High incidence rate of postoperative sore throat in intubated children at Northwest Amhara Comprehensive Specialized Hospitals, Ethiopia. A multicenter study
Authors: Molla Misganaw Terefe , Bizuneh Yosef Belay , Nigatu Yonas Addisu , Melesse Debas Yaregal
Published: March 2023

Background

Postoperative sore throat is the most frequent complication in pediatric patients after general anesthesia. This study aimed to investigate the incidence of postoperative sore throat in patients undergoing general anesthesia with tracheal intubation or laryngeal mask airway.

Methods

A hospital-based multicenter prospective observational cohort study was conducted. Proportional allocation was done with a total of 424 patients from March 1 to June 30, 2022. The information was entered into the Epi-Data software version 4.6 and analyzed with Stata 14. Socio–demographic, surgical, and anesthetic-related characteristics were analyzed using descriptive statistics. A p-value of less than 0.2 was the cutpoint of bivariate logistic regression analysis, and p-values of less than 0.05 were regarded as statistically significant in multivariate logistic regression to determine the presence and strength of association between independent variables and postoperative sore throat.

Results

A total of 411 patients were included in this study, with a response rate of 96.9%. The overall proportion of patients who developed postoperative sore throat was 45% (95% CI: 40.18–49.84). Patients who had anesthesia for more than two hours (AOR = 8.23: 95% CI = 4.08–16.5), those who were intubated by undergraduate anesthesia students (AOR = 2.67: 95% CI = 1.53–4.67), and those who had been intubated using tracheal tube (AOR = 2.38: 95% CI = 1.15–4.92) were significantly associated with the level of postoperative sore throat.

Conclusions and recommendations

We concluded that intubated children with ETT have a high incidence of post-operative sore throat. Tracheal tube usage, intubation by undergraduate students, and more than two hours of anesthesia duration were associated factors. The incidence of sore throat can be decreased with the use of a laryngeal mask airway, intubation by a senior anesthetist, and shortening of anesthesia time.
A nomogram for predicting severe adenovirus pneumonia in children
Authors: Zhang Jiamin , Xu Changdi , Yan Shasha , Zhang Xuefang , Zhao Deyu , Liu Feng
Published: March 2023

Adenoviral pneumonia in children was an epidemic that greatly impacted children's health in China in 2019. Currently, no simple or systematic scale has been introduced for the early identification and diagnosis of adenoviral pneumonia. The early recognition scale of pediatric severe adenovirus pneumonia was established based on an analysis of the children's community-acquired pneumonia clinical cohort. This study analyzed the clinical data of 132 children with adenoviral pneumonia who were admitted to the Children's Hospital of Nanjing Medical University. The clinical parameters and imaging features were analyzed using univariate and multivariate logistic regression analyses. A nomogram was constructed to predict the risk of developing severe adenovirus pneumonia in children. There were statistically significant differences in age, respiratory rate, fever duration before admission, percentage of neutrophils and lymphocytes, CRP, ALT, and LDH between the two groups. Logistic regression analysis was conducted using the R language, and respiratory rate, percentage of neutrophils, percentage of lymphocytes, and LDH were used as scale indicators. Using the ROC curve, the sensitivity and specificity of the scale were 93.3% and 92.1%. This scale has good sensitivity and specificity through internal verification, which proves that screening for early recognition of severe adenovirus pneumonia can be realized by scales. This predictive scale helps determine whether a child will develop severe adenovirus pneumonia early in the disease course.
The diagnostic yield of CGH and WES in neurodevelopmental disorders
Authors: Alotibi Raniah S. , Sannan Naif S. , AlEissa Mariam , Aldriwesh Marwh G. , Al Tuwaijri Abeer , Akiel Maaged A. , Almutairi Mashael , Alsamer Alhanouf , Altharawi Nouf , Aljawfan Ghadah , Alotiabi Badi , AlBlawi Mohammed A. , Alfares Ahmed
Published: March 2023

Background

Neurodevelopmental disorders are a group of conditions characterized by developmental delays leading to abnormal brain functions. The methods of diagnosis and treatment of these conditions are complicated, and their treatment involves a combination of various forms of therapy. In recent years, the development of high-resolution technologies has played an important role in revealing the microdeletions, microduplications, and single-nucleotide variants of the chromosomes and how they are linked to the development of neurodevelopmental disorders. The wide implementation and application of molecular methodologies have started to shed light on the functional importance of using the appropriate methods in detecting these genetic variations that are categorized as either pathogenic or benign. The study aimed to compare the diagnostic yield of comparative hybridization (CGH) and whole exome sequencing (WES) in neurodevelopmental disorders among children attending the King Abdullah Specialist Children Hospital, Riyadh, Saudi Arabia.

Methods

A retrospective study was conducted between 2015 and 2018 on 105 patients diagnosed with neurodevelopmental disorders through array-based CGH (Array-CGH) and WES.

Results

In a sample of 105 patients, 16% was the hit rate of copy number variations (CNVs). WES was requested for CNV-negative patients (n = 79), of which 30% was the hit rate of pathogenic or likely pathogenic single-nucleotide variants. There was a difference in the diagnostic yield between CGH (16%) and WES (30%).

Conclusion

WES was a better approach than Array-CGH to detect various DNA mutations or variants. Our findings could guide clinicians, researchers, and testing laboratories select the most cost-effective and appropriate approach for diagnosing their patients.
Chebulagic acid suppresses gastric cancer by inhibiting the AURKA/β-catenin/Wnt pathway
Authors: Zhao Jing , Shi Yunfu , Ma Yubo , Pan Libin , Wang Yanan , Yuan Li , Dong Jinyun , Ying Jieer
Published: March 2023

Gastric cancer (GC) is a prevalent malignant neoplasm that poses a serious threat to human health. Overexpression of Aurora A (AURKA) is frequently associated with the self-renewal and tumorigenicity of various cancers. Chebulagic acid (CA) has been examined as a potential tumor suppressor based on its ability against numerous tumor biological activities. However, the possible mechanisms of CA inhibition of the progression of GC by mediating the AURKA/β-catenin/Wnt signaling pathway have not been investigated. The present study investigated the level of AURKA expression in GC. We further examined the effect of CA on cell proliferation, migration, and apoptosis in the MKN1 and NUGC3 GC cell lines, and its efficacy in suppressing tumor growth was assessed in tumor bearing mice model. We demonstrated that AURKA was highly expressed in GC and associated with poor prognosis. We demonstrated that treatment with CA significantly inhibited the proliferation and migration of GC cells and induced apoptosis. Compared to the vehicle group, CA treatment severely diminished the volume and weight and the metastasis of tumors. CA also inhibited the expression of AURKA and the AURKA/β-catenin/Wnt signaling pathway in vitro and in vivo. Collectively, the present results demonstrated that high expression of AURKA may be an independent factor of poor prognosis in patients with GC, and CA significantly suppressed the tumor biological functions of GC and inhibited the AURKA/β-catenin/Wnt pathway.
Efficacy and safety of praziquantel preventive chemotherapy in Schistosoma mansoni infected school children in Southern Ethiopia: A prospective cohort study
Authors: Gebreyesus Tigist Dires , Makonnen Eyasu , Tadele Tafesse , Mekete Kalkidan , Gashaw Habtamu , Gerba Heran , Aklillu Eleni
Published: March 2023

Background: The World Health Organization recommends efficacy and safety surveillance of anti-helminths used in mass drug administration campaigns. We evaluated the effectiveness of single-dose praziquantel against Schistosoma mansoni infection, and the safety of praziquantel plus albendazole preventive chemotherapy (PC) in Schistosoma mansoni infected school children (n = 512) in Southern Ethiopia.

Method: Stool examinations were done using thick smear Kato-Katz at baseline, week-4, and week-8 of post-Mass drug administration (MDA) to assess praziquantel efficacy. Participants were followed for MDA-associated adverse events up to day 7 of post-MDA. The primary and secondary study outcomes were praziquantel efficacy (parasitological cure and egg reduction rates) and MDA-associated adverse events (AEs), respectively.

Result: The overall cure rates at week-4 and week-8 were 89.1% (95%CI = 86.1–91.7) and 87.5% (95%CI = 83.6–90.8), respectively. Cure rates among moderate-to-heavily infected children were significantly lower (p = 0.001) compared to those with light infection at week-4 (84.4% vs. 91.1%, p = 0.03) and week-8 (78.6% vs. 91.9%, respectively). Older children had a higher cure rate than younger ones at week-8 (90.1% vs. 79.5%, p = 0.01). Among those who were Schistosoma egg-free (cured) at week 4, 7.8% became egg-positive at week 8. The overall egg reduction rate (ERR) at week-4 and week-8 were 93.5% and 91.3%, respectively, being lower among the 5–9 years old age groups (p = 0.01) at week-8. The proportion of children who remained schistosoma egg-positive throughout the study follow-up period was 4.6%, and their ERR at week-4 and week-8 was 50% and 51%, respectively, which is below the 90% World Health Organization threshold for efficacy. The incidence of experiencing at least one type of MDA-associated AEs were 17.0% (95%CI = 13.8%–20.5%); abdominal pain, headache, and vomiting were the most common. The proportion of mild, moderate, and severe AEs was 63.2%, 26.3%, and 10.5%, respectively. Females experienced more AEs than males (p = 0.03).

Conclusion: Single-dose praziquantel is still effective for the treatment of intestinal schistosomiasis. Praziquantel and albendazole preventive chemotherapy is safe and tolerable, and associated AEs are mostly mild-to-moderate and transient. However, the reduced PZQ effectiveness in moderate-to-heavy infection and observed AEs in about one-fifth of infected children underscores the need for better treatment strategies and surveillance for early detection of parasite resistance and management of AEs.
Characterization of a bimodal multi-fibre optic clinical probe for in situ tissue diagnosis based on spectrally-and temporally-resolved autofluorescence
Authors: Sibai Mira , Mehidine Hussein , Devaux Bertrand , Abi Haidar Darine
Published: March 2023

Introduction: Fibred-based optical spectroscopy is advantageous over imaging due to its sensitivity, practicality and precision, providing point of care diagnosis. The unique advantage is that the sampled volume is well defined by the source-detector geometry and that the functionality of multiple optical techniques can be incorporated into one probe so that more information is gained without extra bulkiness or cost, while also mitigating the limitations of each.

Methods: This advantage is utilized here to address the limited accuracy in delineating brain tumors, in situ, by simultaneously characterizing tissue based on the spectral and lifetime properties of five endogenous fluorophores commonly present in brain tissue. A 5-meters multi fibre-optic probe custom-built for neurosurgery guidance with a sterilizable distal end is presented in this paper. It describes its technical features such as architecture, collection efficiency, sensitivity.

Results and discussion: The developed probe have a temporal resolution of 34.6 ps and a temporal impulse response function of 29 ps. The spatial resolution was around 153 μm while the spectral resolution is 1 nm. All of these specifications are ensured under 1 second as acquisition time. The probe is validated using fluorescent dyes and tissue phantoms. Additionally, the fluorescence spectra from fresh and frozen animal tissue and from an epileptic patient are compared to that obtained from confocal fluorescence microscopy, while the lifetime values are compared to that reported in literature. Finally, the effect of pressure applied to the probe in contact with tissue is discussed.
Transcriptome and molecular regulatory mechanisms analysis of gills in the black tiger shrimp Penaeus monodon under chronic low-salinity stress
Authors: Li Yun-Dong , Si Meng-Ru , Jiang Shi-Gui , Yang Qi-Bin , Jiang Song , Yang Li-Shi , Huang Jian-Hua , Chen Xu , Zhou Fa-Lin , Li ErChao
Published: March 2023

Background: Salinity is one of the main influencing factors in the culture environment and is extremely important for the survival, growth, development and reproduction of aquatic animals.

Methods: In this study, a comparative transcriptome analysis (maintained for 45 days in three different salinities, 30 psu (HC group), 18 psu (MC group) and 3 psu (LC group)) was performed by high-throughput sequencing of economically cultured Penaeus monodon. P. monodon gill tissues from each treatment were collected for RNA-seq analysis to identify potential genes and pathways in response to low salinity stress.

Results: A total of 64,475 unigenes were annotated in this study. There were 1,140 upregulated genes and 1,531 downregulated genes observed in the LC vs. HC group and 1,000 upregulated genes and 1,062 downregulated genes observed in the MC vs. HC group. In the LC vs. HC group, 583 DEGs significantly mapped to 37 signaling pathways, such as the NOD-like receptor signaling pathway, Toll-like receptor signaling pathway, and PI3K-Akt signaling pathway; in the MC vs. HC group, 444 DEGs significantly mapped to 28 signaling pathways, such as the MAPK signaling pathway, Hippo signaling pathway and calcium signaling pathway. These pathways were significantly associated mainly with signal transduction, immunity and metabolism.

Conclusions: These results suggest that low salinity stress may affect regulatory mechanisms such as metabolism, immunity, and signal transduction in addition to osmolarity in P. monodon. The greater the difference in salinity, the more significant the difference in genes. This study provides some guidance for understanding the low-salt domestication culture of P. monodon.
Ectopic expression of the AtCDF1 transcription factor in potato enhances tuber starch and amino acid contents and yield under open field conditions
Authors: Carrillo Laura , Baroja-Fernández Edurne , Renau-Morata Begoña , Muñoz Francisco J. , Canales Javier , Ciordia Sergio , Yang Lu , Sánchez-López Ángela María , Nebauer Sergio G. , Ceballos Mar G. , Vicente-Carbajosa Jesús , Molina Rosa V. , Pozueta-Romero Javier , Medina Joaquín
Published: March 2023

Introduction

Cycling Dof transcription factors (CDFs) have been involved in different aspects of plant growth and development. In Arabidopsis and tomato, one member of this family (CDF1) has recently been associated with the regulation of primary metabolism and abiotic stress responses, but their roles in crop production under open field conditions remain unknown.

Methods

In this study, we compared the growth, and tuber yield and composition of plants ectopically expressing the CDF1 gene from Arabidopsis under the control of the 35S promoter with wild-type (WT) potato plants cultured in growth chamber and open field conditions.

Results

In growth chambers, the 35S::AtCDF1 plants showed a greater tuber yield than the WT by increasing the biomass partition for tuber development. Under field conditions, the ectopic expression of CDF1 also promoted the sink strength of the tubers, since 35S::AtCDF1 plants exhibited significant increases in tuber size and weight resulting in higher tuber yield. A metabolomic analysis revealed that tubers of 35S::AtCDF1 plants cultured under open field conditions accumulated higher levels of glucose, starch and amino acids than WT tubers. A comparative proteomic analysis of tubers of 35S::AtCDF1 and WT plants cultured under open field conditions revealed that these changes can be accounted for changes in the expression of proteins involved in energy production and different aspects of C and N metabolism.

Discussion

The results from this study advance our collective understanding of the role of CDFs and are of great interest for the purposes of improving the yield and breeding of crop plants.
Red fluorescence protein (DsRed2) promotes the screening efficiency in peanut genetic transformation
Authors: Huai Dongxin , Wu Jie , Xue Xiaomeng , Hu Meiling , Zhi Chenyang , Pandey Manish K. , Liu Nian , Huang Li , Bai Dongmei , Yan Liying , Chen Yuning , Wang Xin , Kang Yanping , Wang Zhihui , Jiang Huifang , Lei Yong , Varshney Rajeev K. , Liao Boshou
Published: March 2023

Peanut (Arachis hypogaeaL.), one of the leading oilseed crops worldwide, is an important source of vegetable oil, protein, minerals and vitamins. Peanut is widely cultivated in Asia, Africa and America, and China is the largest producer and consumer of peanut. Genetic engineering has shown great potential to alter the DNA makeup of an organism which is largely hindered by the low transformation and screening efficiency including in peanut. DsRed2 is a reporter gene widely utilized in genetic transformation to facilitate the screening of transformants, but never used in peanut genetic transformation. In this study, we have demonstrated the potential of the red fluorescence protein DsRed2 as a visual reporter to improve screening efficiency in peanut. DsRed2 was firstly expressed in protoplasts isolated from peanut cultivar Zhonhua 12 by PEG, and red fluorescence was successfully detected. Then, DsRed2 was expressed in peanut plants Zhonghua 12 driven by 35S promotervia Agrobacterium tumefaciens-mediated transformation. Red fluorescence was visually observed in calli and regenerated shoots, as well as in roots, leaves, flowers, fresh pod shells and mature seeds, suggesting that transgenic screening could be initiated at the early stage of transformation, and continued to the progeny. Upon screening with DsRed2, the positive plant rate was increased from 56.9% to 100%. The transgenic line was then used as the male parent to be crossed with Zhonghua 24, and the hybrid seeds showed red fluorescence as well, indicating that DsRed2 could be applied to hybrid plant identification very efficiently. DsRed2 was also expressed in hairy roots of Huayu 23via Agrobacterium rhizogenes-mediated transformation, and the transgenic roots were easily selected by red fluorescence. In summary, the DsRed2 is an ideal reporter to achieve maximum screening efficiency and accuracy in peanut genetic transformation.
Will genetically modified late blight resistant potatoes be the first GM crops to be approved for commercial growing in Norway?
Authors: Forbes Edward , Wulff-Vester Anders Keim , Hvoslef-Eide Trine (A.K.)
Published: March 2023

Last decade’s advances in biotechnology, with the introduction of CRISPR, have challenged the regulatory framework for competent authorities all over the world. Hence, regulatory issues related to gene editing are currently high on the agenda both in the EU and in the European Economic Area (EEA) Agreement country of Norway, particularly with regards to sustainable agriculture. During the negotiations on the EEA Agreement, Norway was allowed to retain three extra aims in the Gene Technology Act: “That the production and use of GMO happens in an ethical way, is beneficial to society and is in accordance with the principle of sustainable development”. We argue the case that taking sustainability into the decisions on regulating gene edited products could be easier in Norway than in the EU because of these extra aims. Late blight is our chosen example, as a devastating disease in potato that is controlled in Norway primarily by high levels of fungicide use. Also, many of these fungicides are being banned due to negative environmental and health effects. The costs of controlling late blight in Norway were calculated in 2006, and since then there have been new cultivars developed, inflation and an outbreak of war in Europe increasing farm input costs. A genetically modified (GM) cisgenic late blight resistant (LBR) potato presents a possible solution that could reduce fungicide use, but this could still be controversial. This paper aims to discuss the advantages and disadvantages of approving the commercial use of a GM LBR potato cultivar in Norway and compare these against currently used late blight management methods and conventional potato resistance breeding. We argue that a possible route for future regulatory framework could build upon the proposal by the Norwegian Biotechnology Advisory Board from 2019, also taking sustainability goals into account. This could favour a positive response from the Competent Authorities without breeching the European Economic Area (EEA) Agreement. Perhaps the EU could adopt a similar approach to fulfil their obligations towards a more sustainable agriculture?
Staying physically active during the COVID-19 pandemic: Assessing the roles of motivation, basic psychological needs, goal orientation and anticipatory sport persistence
Authors: Loerbroks Lotta , Kersten Louisa Joyce , Freund Philipp Alexander
Published: March 2023

Nationwide barriers to public and private sport institutions were implemented during COVID-19 lockdowns. Autonomous motivation, perceived fulfillment of basic psychological needs (competence, autonomy, and relatedness) and goal orientation coincide with higher persistence rates in physical activity. The aim of this study is to investigate which factors are related to anticipatory sport persistence, a specific form of sport persistence. We conducted an online survey with N = 208 (74% female) participants. Correlation analyses showed that higher anticipatory sport persistence coincides with autonomous motivation (r = 0.314, p < 0.01), basic psychological needs (competence r = 0.528, autonomy r = 0.446, relatedness r = 0.315; all p < 0.01), and goal orientation (intrinsic r = 450, extrinsic r = 0.146; all p < 0.01). Furthermore, multiple regression analysis showed that anticipatory sport persistence can be predicted through intrinsic goal orientation (B = 0.465, p < 0.01) and the need for competence (B = 0.418, p < 0.01). The importance of anticipatory sport persistence when expecting external barriers to physical activity, its relationship toward sport persistence and possible implications for the planning and perseverance of physical activity plans are being discussed.
Holding open spaces to explore beyond: Toward a different conceptualization of specialization in high-performance sport
Authors: Richard Veronique , Cairney John , Woods Carl T.
Published: March 2023

This conceptual analysis aims to challenge the state of high-performance sport by questioning the concept of specialization. To start, we offer a brief, but critical overview of what specialization currently entails. Then, shifting the paradigm, we suggest an expansion rather than a reduction of developmental possibilities once an athlete reaches the “top”. Specifically, rather than athletes conforming to national standards imposed by governing bodies about what it means to be “elite”, we suggest sport systems consider a person-environment fit approach to support ongoing development. Drawing on an ecological dynamics rationale and various socio-cultural theories, we explore how concepts such as affordances and perspectives can be harnessed to create a better “fit” between athletes’ action capabilities and the opportunities within their broader environment. Our conception of specialization requires moving away from a definition of success based on the accumulation of medals, toward one that accounts for the exploration and achievement of the possible. We argue that a person-environment fit welcomes diversity, so long as it sustains the person’s health, wellbeing, and performance. This, it is suggested, is about collectively holding open spaces for each other to explore beyond the constraints of high-performance sport, encouraging all to carry on their lives in directions meaningfully impactful for them. We conclude this conceptual analysis with a brief case example demonstrating what our theorizing could look like in practice.
Psychedelic replications in virtual reality and their potential as a therapeutic instrument: an open-label feasibility study
Authors: Kaup Karl Kristjan , Vasser Madis , Tulver Kadi , Munk Mari , Pikamäe Juhan , Aru Jaan
Published: March 2023

Background

Recent research has shown promising results for the therapeutic benefits of psychedelics. One popular view claims that these benefits are mediated by the subjective experiences induced by these substances. Based on this, we designed a virtual reality experience, Psyrreal, that mimics the phenomenological components of psychedelic experiences.

Aims

We aimed to investigate the therapeutic efficacy of Psyrreal and psychedelic VR experiences in treating depressive symptoms as well as explore the effect of Psyrreal on subjective factors which have been suggested to mediate the therapeutic benefits of psychedelics.

Methods

In this open-label feasibility study, thirteen participants with mild-to-moderate depression underwent a 2-day therapeutic intervention implementing Psyrreal. Depressive symptoms were evaluated by the Emotional State Questionnaire (EST-Q2) at the start of the intervention and 2 weeks after. A thematic analysis of semi-structured interviews after Psyrreal was also conducted as an additional assessment of the method.

Results

A 2-day intervention implementing Psyrreal led to significant decreases in depressive symptoms at the 2-week follow-up (n = 10, p = 0.007, Hedges’ g = 1.046) measured by the Emotional State Questionnaire (EST-Q2). The analysis of semi-structured interviews suggests that Psyrreal could lead to insight and alterations in the sense of self in some people.

Conclusion

This work proposes a novel method using virtual reality to augment the treatment of psychological disorders as well as to precisely investigate the mediating subjective factors of the therapeutic effects of psychedelic substances. Our preliminary results suggest that VR experiences combined with psychological support show potential in treating depressive symptoms and further research into similar methods is warranted.
Factors associated with hopelessness, depression and anxiety in the Honduran-Central America population during the COVID-19 pandemic
Authors: Espinoza-Turcios Eleonora , Gonzales-Romero René Mauricio , Sosa-Mendoza Carlos , Sierra-Santos Manuel , Castro-Ramos Henry Noel , Zambrano Lysien Ivania , Armada José , Mejía Christian R.
Published: March 2023

Introduction

The mental health of the population has been affected by COVID-19, reporting in many populations higher levels of depressive, anxious and stress symptoms, however, in Honduras there are no studies showing the impact of COVID-19 on the mental health of the population.

Aim

To determine the factors associated with hopelessness, depression and anxiety in times of COVID-19 in the Honduran population.

Methods

Cross-sectional analytical study. Three scales were used, Beck for hopelessness, Hamilton for depression and anxiety, through interviews with the population attending different levels of health care throughout the country. Descriptive and analytical statistics were obtained. For hopelessness, the Beck Hopelessness Scale was used; for depression and anxiety, the Hamilton Scale was used.

Results

Of the 8,125 participants, the population in general showed results of hopelessness 14.9%, depression 12.9% and anxiety 1.2%, there was less hopelessness among women (p = 0.004), university students (p < 0.001), but greater among those who had a family member deceased by COVID-19 (p < 0.001), among those who had diabetes mellitus (DM) (p < 0.001), history of mental illness (p < 0.001) or had consumed drugs in the last 6 months (p < 0.001). There was more depression among those with arterial hypertension (p = 0.002), obesity (p = 0.019), DM (p = 0.004), history of mental illness (p < 0.001) or had consumed drugs in the last 6 months (p < 0.001). There was more anxiety among those infected with COVID-19 (p = 0.023), according to having a family member deceased by COVID-19 (p = 0.045) and in those with a history of mental illness (p < 0.001).

Discussion

The Honduran population presented important differences in the socio-pathological, according to the loss of family members or having previous illnesses, this according to the presentation of problems in the mental sphere.

Conclusion

We found factors associated with hopelessness, we found factors associated with hopelessness, depression and anxiety in times of COVID-19 in the Honduran population.
Resources availability and COVID-19 mortality among US counties
Authors: Epané Josué Patien , Zengul Ferhat , Ramamonjiarivelo Zo , McRoy Luceta , Weech-Maldonado Robert
Published: March 2023

The COVID-19 was declared a pandemic by WHO on 03/2020 has claimed millions of lives worldwide. The US leads all countries in COVID-19-related deaths. Individual level (preexisting conditions and demographics) and county-level (availability of resources) factors have been attributed to increased risk of COVID-19-related deaths. This study builds on previous studies to assess the relationship between county-level resources and COVID-19 mortality among 2,438 US counties. We merged 2019 data from AHA, AHRF, and USA FACTS. The dependent variable was the total number of COVID-19-related deaths. Independent variables included county-level resources: (1) hospital staffing levels (FTE RNs, hospitalists, and intensivists) per 10,000 population; (2) hospital capacity (occupancy rate, proportion of teaching hospitals, and number of airborne infection control rooms per 10,000 population); and (3) macroeconomic resources [per capita income and location (urban/rural)]. We controlled for population 65+, racial/ethnic minority, and COVID-19 deaths per 1,000 population. A negative binomial regression was used. Hospital staffing per 10,000 population {FTE RN [IRR = 0.997; CI (0.995–0.999)], FTE hospitalists [IRR = 0.936; CI (0.897–0.978)], and FTE intensivists [IRR = 0.606; CI (0.516–0.712)]} was associated with lower COVID-19-related deaths. Hospital occupancy rate, proportion of teaching hospitals, and total number of airborne infection control rooms per 10,000 population were positively associated with COVID-19-related deaths. Per capita income and being in an urban county were positively associated with COVID-19-related deaths. Finally, the proportion of 65+, racial/ethnic minorities, and the number of cases were positively associated with COVID-19-related deaths. Our findings suggest that focusing on maintaining adequate hospital staffing could improve COVID-19 mortality.
Creating healthy workplaces in healthcare: Are we delaying progress by focusing on what we can do rather than what we should do?
Authors: Montgomery Anthony , Lainidi Olga
Published: March 2023

All the available evidence points to the fact that healthcare is under considerable stress, and while change is urgently needed there is no quick fix; systemic and sustained changes in organizational cultures within healthcare are required. Moreover, the fragility of healthcare systems globally has been starkly exposed by the Coronavirus 2019 pandemic. We have gathered enough evidence to know what is driving poor wellbeing, and how these processes impact on quality of care and patient safety. Indeed, we have a good idea of what we need to do to improve the situation. Therefore, this begs a simpler question; If we know how to create healthy workplaces, why is it so difficult to achieve this in healthcare? In the following perspective paper, we will argue that we can do better if we address the following three issues: (1) we are ignoring the real problems, (2) limited successes that we are achieving are moving us further from tackling the real problems, (3) culture change is accepted as crucial, but we are not accepting what the evidence is telling us about healthcare culture. Tackling burnout is useful and necessary, but we must increase dignity among healthcare employees. Moreover, we need to train line managers to recognize and facilitate the need of employees to feel competent and be appreciated by others, while helping them set wellbeing boundaries.
Dentists' lived experience of providing dental care during the COVID-19 pandemic: A qualitative study in Mashhad, Iran
Authors: Hoseinzadeh Melika , Sa’adAbadi Zahra , Maleki Kambakhsh Sara , Babazadeh Saber
Published: March 2023

Background

This study aimed to explore the lived experiences of dentists practicing in Mashhad, Iran, during the COVID-19 pandemic and identify possible influencing factors for providing dental health in this era.

Methods

Sixteen dentists took part in this qualitative phenomenological study. Semi-structured face-to-face interviews were performed and evaluated using a qualitative content analysis approach. The MAXQDA program (2020) was used to code and classify the data. Purposive sampling, audio recording, member checking, and peer review were employed to verify transferability, reliability, and validity. The interview transcripts were transcribed, and sentences and remarks relevant to the study's goal were retrieved and classified.

Results

Six hundred twenty-eight codes were extracted after several readings of the texts. Five core themes and fourteen sub-themes were determined after deleting repeated themes and axial coding. The main five themes included: “Coping with the COVID-19 pandemic”, “Alternations in providing dental care,” “Infection control,” “COVID-19-related news and information sources”, and “Positive and negative aspects of COVID-19 in dental care ”.

Conclusions

During the COVID-19 pandemic, dentists noted various challenges in delivering routine dental treatment, including a lack of resources, reliable and straightforward guidelines for dentists, and governmental policies for dental facility restrictions. Dentists concluded that financial, educational, and financial support from the government would significantly enhance the delivery of community dental care services. With the right strategies and lessons learned, dentists may be better prepared for future challenges in global health care.
Technical skills in complex tennis situations: Dutch talented players U15 compared to players U17
Authors: Kolman Nikki S. , Huijgen Barbara C. H. , Visscher Chris , Elferink-Gemser Marije T.
Published: March 2023

Introduction

Technical skills in complex situations appear crucial for progress towards elite tennis performance. However, it is unknown how these skills develop in different age categories in a group of talented youth players. The aim of this study is to evaluate possible differences in technical skills among Dutch talented youth tennis players U15 compared to U17.

Methods

A total of 19 players (12 males, 7 females; age 14.6 ± 1.4 years) were tested on ball speed, accuracy, percentage errors and spin rate using the on-court Dutch Technical-Tactical Tennis Test. With a ball machine, four games were simulated which were either fixed (game 1 and game 2) or variable (game 3 and game 4), depending on the complexity of the task. Each game consisted of two offensive, two neutral and two defensive rallies, representing different tactical situations.

Results

A two-way ANOVA revealed a statistically significant interaction between the effects of age category and sex for ball speed (F(1,15) = 5.472, p = 0.034, η2 = 0.267), indicating that males U17 produced higher ball speed compared to males U15, whereas no differences were found between females U15 and U17. A one-way ANCOVA showed that, regardless of sex, players U17 scored significantly higher on accuracy than players U15 (F(1,16) = 5.021, p = 0.040, η2 = 0.239). No differences were found between players U15 and U17 for spin rate and percentage errors (p > .05), although there was a medium to large effect size for males U17 to produce higher spin rates compared to males U15. A closer examination of accuracy revealed that players U17 scored significantly higher compared to players U15 in game 4 (F(1,17) = 6.358, p = .022, η2 = .272) and in defensive situations (F(1,17) = 9.602, p = .007, η2 = .361).

Discussion

In conclusion, the results of the current study suggest that technical skills, especially ball speed for males and accuracy in complex situations for both males and females, continue to develop in adolescence in talented tennis players. There is an increased understanding about underlying technical skills that contribute to progress towards elite tennis performance. To effectively develop technical skills, coaches are encouraged to design specific practices where these skills are performed in complex situations under high cognitive and temporal pressure.
Pooches on a platform: Text mining twitter for sector perceptions of dogs during a global pandemic
Authors: McMillan Kirsten M. , Anderson Katharine L. , Christley Robert M.
Published: March 2023

Introduction

Businesses commonly text mine Twitter data to identify patterns and extract valuable information. However, this method is rarely applied to the animal welfare sector. Here, we describe Twitter conversations regarding dogs during a global pandemic, assess the evolution of sentiment, and examine the dynamics of sector influence.

Methods

Between March and August 2020, we gathered 61,088 unique tweets from the United Kingdom and Republic of Ireland, relating to COVID-19 and dogs. Tweets were assigned to one of four pandemic phases and active accounts were assigned to a sector: Personal (i.e., UK and ROI public), Press (i.e., mass media), State (i.e., Government, Police, and NHS), and Other (i.e., welfare organizations, social enterprises, research organizations, charity, and business).

Results

Word frequency and sentiment analysis between phases and sectors were assessed, and cross correlation functions and lagged regressions were used to evaluate sector influence. Topical foci of conversations included: meat trade, separation anxiety and dog theft. Sentiment score remained stable until the last phase where sentiment decreased (F3, 78, 508= 44.4,p< 0.001), representing an increased use of negative language. Sentiment differed between the four sectors (F3, 11, 794= 52.2,p< 0.001), with Personal and Press accounts presenting the greatest use of negative language. Personal accounts were initially partly influenced by State accounts (R= −0.26;p= 0.05), however this altered to Press accounts by the last phase (R= −0.31;p= 0.02).

Discussion

Our findings highlight that whilst Personal accounts may affect sector-specific messaging online, perhaps more importantly: language used, and sentiment expressed by Press, State and Other accounts may influence public perception. This draws attention to the importance of sector responsibility regarding accurate and appropriate messaging, as irresponsible/ill-considered comments or campaigns may impact future human-animal interaction.
Ballistic study on the penetration potential and injury potential of different bullet types in the use of a newly developed bullet shooting stunner for adequate stunning of heavy cattle
Authors: Gascho Dominic , Stephan Roger , Zoelch Niklaus , Vogt Michael , Oesch Michelle Aimée , Thali Michael , Richter Henning
Published: March 2023

Introduction

Recently, a special bullet shooting stunner for heavy cattle has been developed that fires a bullet instead of a bolt. In the search for a suitable ammunition, the following criteria must be met: First, the energy of the bullet must be sufficient to penetrate the thick frontal bones of heavy cattle. Second, the injury potential at the corresponding penetration depth should preferably be large in order to damage brain tissue relevant to stunning. Third, the bullet must not perforate the occipital bone (over-penetration).

Methods

Four different bullet types [Hornady FTX, Hydra-Shok, Black Mamba, and a common full metal jacket (FMJ) bullet] were evaluated in a series of experiments on soap blocks and removed bone plates followed by computed tomography examinations. Penetration potential was evaluated in terms of kinetic energy relative to the caliber of the bullet, i.e., mean energy density (ED). Injury potential was evaluated by the mean extent of the cavity volume (eCV) at the relevant penetration depth of 5.5 to 7.5 cm in the soap block.

Results

All four bullet types passed through the frontal bone plate. The ED was 17.50 J/mm2 (Hornady FTX), 17.46 J/mm2 (Hydra-Shok), 13.47 J/mm2 (Black Mamba), and 13.47 J/mm2 (FMJ). The Hornady FTX and the Hydra-Shok each fragmented heavily. The FMJ was excluded after three experiments due to over-penetrations. The eCV was eCV = 3.77 cm2 (Hornady FTX), 2.71 cm2 (Hydra-Shok), and 1.31 cm2 (Black Mamba), with a significant difference (p = 0.006) between the Hornady FTX and the Black Mamba.

Discussion

For use in heavy cattle, the Hornady FTX and the Hydra-Shok are recommended due to the larger eCV than the Black Mamba.
Inoculation with Plant Growth-Promoting Bacteria Improves the Sustainability of Tropical Pastures with Megathyrsus maximus
Authors: Guimarães Gabriel Silva , Rondina Artur Berbel Lirio , de Oliveira Junior Admilton Gonçalves , Jank Liana , Nogueira Marco Antonio , Hungria Mariangela
Published: March 2023

Brazil is the second-largest producer and the first exporter of beef, with herds mainly raised in extensive pastures, where Megathyrsus maximus occupies over 30 Mha. About 70% of the pastures are under degradation, and using plant growth-promoting bacteria (PGPB) may contribute to reversing this scenario. We investigated the effects of PGPB on the growth of six cultivars of M. maximus—Tanzania-1, Massai, BRS Zuri, Mombaça, BRS Tamani, and BRS Quênia—under greenhouse conditions. Plants were inoculated, or not, with the elite strains of Azospirillum brasilense CNPSo 2083 + CNPSo 2084, Bacillus subtilis CNPSo 2657, Pseudomonas fluorescens CNPSo 2719, or Rhizobium tropici CNPSo 103. At 35 days after emergence, plants were evaluated for ten root growth traits, shoot dry weight, and the levels of macro and micronutrients accumulated in shoots. Several root traits were increased due to inoculation in all genotypes, impacting plant growth and nutrient uptake. Despite the differences in effectiveness, all genotypes benefited from PGPB to some degree, but Mombaça and BRS Zuri were more responsive. Scanning electron microscopy indicated that bacterial species differed in their capacity to colonize seeds and rootlets. The results show that inoculation with elite PGPB strains may represent an important strategy for the sustainability of M. maximus pastures.