bluuebunny/crossref_metadata_2025 huggingface dataset:
The Contribution of Frailty to Participation of Older Adults
Authors: Rand Debbie , Sternberg Shelley A. , Gasner Winograd Reut , Buckman Zvi , Bentur Netta
Published: January 2022

Background: Participation, which is involvement in life situations, is an important indicator of human health and well-being of older adults. Frailty is known to be related to difficulties in activities of daily living (ADL) but the association with participation restriction has not been sufficiently researched. Therefore, we aimed to (1) to assess the correlations between frailty, ADL, and participation; and (2) to identify the contribution of frailty to explaining the participation restriction of older adults. Methods: A cross-sectional study included home visits to community-dwelling older adults aged 75 and older. The Reintegration to Normal Living Index (RNL-I) assessed participation, PRISMA-7 assessed frailty, and the Functional Independence Measure and IADL questionnaire assessed the basic and instrumental ADL. Cognition, which may explain participation, was also assessed (The Montreal Cognitive Assessment) and demographic information was collected. Results: Older adults (N = 121, 60 women), aged 75 to 91 years (mean (SD)—79.6 (3.1)), were included. Older adults demonstrated full to restricted participation (RNL-I-mean (SD)—78.2 (18.0)/100). Frailty was identified in 39 (32%) older adults (mean (SD) PRISMA-7—2.9 (1.4)/7points). A negative moderate significant correlation was found between participation and frailty (r = −0.634, p < 0.001). The variance of participation was significantly explained by frailty, 31.5%, and basic ADL, 5.6% (after controlling for age and cognition); the total model explained 44.6% (F = 23.29, p < 0.001). Conclusions: Frailty is significantly associated with participation restriction. Since participation has many health benefits, understanding which factors are associated to participation is central to developing interventions for older adults. These findings may help health professionals in the future develop interventions for maintaining and promoting the participation of older adults.
The Accumulation and Transformation of Heavy Metals in Sediments of Liujiang River Basin in Southern China and Their Threatening on Water Security
Authors: Miao Xiongyi , Song Mian , Xu Gaohai , Hao Yupei , Zhang Hucai
Published: January 2022

Heavy metal (HM) pollution in sediments is tightly related to the security of water quality in rivers, but the accumulation and conversion of HMs are poorly researched, so that a field study was conducted as an example in the Liujiang River Basin. Seven HMs were analyzed to determine between the overlying water and sediments. Moreover, the regulation of HMs speciation and environmental factors in their accumulation and conversion were identified. The obtained results suggested the HM concentrations in water are far below the primary standard of water quality, but in sediments, the contents of Cd and Zn are significantly higher than their corresponding baseline of soil. Only Cd and Pb are dominantly in non-residual form (carbonate-bound fraction and reducible fraction, respectively). The non-significant correlations suggested pH and Eh may be hard to influence HMs in water, while the significant correlations highlighted the regulations of Eh, organic matter and mean grain size on the accumulation of metals in sediments. The opposite correlations between EC, TDS, pH and Cd confirmed the emission of acid wastewater contributed to the accumulation of Cd in sediment. The conversion of metals between water and sediments were found to be significant only in specific forms of Cd, As, Cu, Zn and Pb, suggesting the conversion of HMs in sediments should be largely regulated by their specific forms. The very high risk disclosed by the higher values of Eri and RI are only found upstream, while the higher risk of Cd should be treated as a critical environmental threat.
Pictorial Assessment of Health-Related Quality of Life. Development and Pre-Test of the PictoQOL Questionnaire
Authors: Brzoska Patrick , Erdsiek Fabian , Aksakal Tuğba , Mader Maria , Ölcer Sabahat , Idris Munzir , Altinok Kübra , Wahidie Diana , Padberg Dennis , Yilmaz-Aslan Yüce
Published: January 2022

The aim of the present study was to develop a pictorial questionnaire for the assessment of health-related quality of life (PictoQOL) and to examine its content validity and usability across three exemplary population groups of different origin residing in Germany (non-migrants, Turkish migrants and Arabic-speaking migrants). A mixed-methods design combining qualitative and quantitative methods was used, comprising 6 focus group discussions with a total of 17 participants, 37 cognitive interviews and a quantitative pretest with 15 individuals. The PictoQOL consists of a pictorial representation of a total of 15 different situations. Using a visual Likert scale, respondents indicate how much each situation applies to them. Some representations proved to be culturally sensitive and were adapted. Respondents found the use of an additional graphic layer in the form of symbols in addition to pictures helpful for interpretation. The PictoQOL is considered to allow a more accessible assessment and better comparability of HRQOL across different population groups regardless of their literacy level. It is therefore considered to be superior to existing instruments for routine use in health research and practice. Future studies need to examine its convergent and factorial validity.
Losses in the Sputum Specimen Referral Cascade in Mpulungu District, Zambia: A Cross-Sectional Study
Authors: Goma Ruth , Bwembya Josphat , Mwansa Brian , Ndubani Phillimon , Kasongo Francis , Siame William , Mulenga Lutinala , Kumar Ramya , Kaminsa Seraphine , Makwambeni Vimbai , Musonda Victoria , Thior Ibou , Mwinga Alwyn
Published: January 2022

Sputum specimen referral cascades in resource-limited settings are characterized by losses of specimens, resulting in delays in tuberculosis (TB) diagnosis. Mpulungu District Health Office in Zambia conducted a quantitative based cross-sectional study using both primary and secondary data to identify points at which loss of specimens occurred in the sputum referral cascade. Primary data were collected through observations and interviews with 22 TB service providers. Secondary data were collected through examination of patient files and presumptive TB and laboratory registers to retrospectively track sputum specimens referred by ten health centers from April to September 2018. Proportions of specimens/laboratory results at every stage of the referral cascade were calculated using Epi Info v7. Only 49 (23%) out of 209 sputum specimens completed the referral cascade. The remaining 160 (76%) were lost at various stages of the referral cascade. The largest loss (51%) occurred between the release of laboratory results by the diagnostic facility and their receipt at referring facilities. Barriers included an inadequate number of staff oriented in sputum specimen referral, negative staff attitudes, and lack of specimen packaging material and specimen transportation. The district health office should strengthen the sputum specimen referral system by providing transport and specimen packaging material and by training staff in sputum collection transportation and tracking.
A Field Study on Safety Performance of Apron Controllers at a Large-Scale Airport Based on Digital Tower
Authors: Zhang Jianping , Tian Xiaoqiang , Pan Jian , Chen Zhenling , Zou Xiang
Published: January 2022

The innovative concept of digital tower provides a new solution for reducing the construction and operation costs of airports with adverse natural environments, poor intervisibility conditions, or sparse traffic. However, it leads to changes in the situational awareness of air traffic controllers and to challenges in safety performance. To research the safety performance of apron controllers at a large-scale airport applying a digital tower, a field study was conducted at Baiyun International Airport in Guangzhou, China. In this study, we established a comprehensive index system from the perspective of situational awareness, which provided measurements on the areas of interests, gaze and physiological features, and vigilance of controllers. Three modules were compared: a physical tower module, a digital tower module with a large panoramic screen, and a digital tower module with a small panoramic screen. The differences in the safety performances of apron controllers are discussed in two aspects: adaptability and reliability. The results indicated that the apron controllers at the three modules performed different cognition patterns, but similar cognition effort was paid toward maintaining performance. Furthermore, the significant vigilance decrement of controllers exists between after-duty and before-duty, but with no significant difference among the three modules. In conclusion, apron controllers at a large-scale airport could obtain effective safety performances based on a digital tower that were no less than those from a physical tower.
Street Tree Planning to Improve Public Health and Ecosystem Resilience in Urban Areas: A Scenario Analysis Using a System Dynamics Model
Authors: Seok Youngsun , Yim Hyosook , Moon Taehyeon , Chon Jinhyung
Published: January 2022

Increases in particulate matter in cities threaten both public health and ecosystems. Street trees, which are a corridor-type green infrastructure capable of absorbing particulate matter, have been promoted as one possible solution to this problem. However, planting selected trees solely with the goal of reducing particulate matter may adversely affect street tree ecosystem resilience by inhibiting species diversity. This study aims to investigate urban street tree planting strategies that reduce particulate matter while maintaining ecosystem resilience. To this end, a study site in Suwon, South Korea was selected, and street tree planting scenarios were developed based on the selected site information. A scenario analysis was conducted using a system dynamics model. The model simulated the long-term trends under each scenario regarding the amount of particulate matter absorbed by the trees and the changes in species diversity. The analysis results clearly show that strategic planting of street trees while focusing on only a specific purpose—reducing particulate matter—can adversely affect ecosystem resilience. The scenario analysis also revealed that increasing the number of street trees while maintaining a balance among various species is the best option for reducing particulate matter without degrading species diversity. Additionally, the results support the need to plant evergreen species to consider the winter season.
Oral Health–Related Quality of Life and Missing Teeth in an Adult Population: A Cross-Sectional Study from Poland
Authors: Rodakowska Ewa , Jamiolkowski Jacek , Baginska Joanna , Kaminska Inga , Gabiec Katarzyna , Stachurska Zofia , Kondraciuk Marcin , Dubatowka Marlena , Kaminski Karol Adam
Published: January 2022

The aim of the study was to determine oral health–related quality of life (OHRQoL) using the measures Geriatric/General Oral Health Assessment (GOHAI) and Oral Health Impact Profile (OHIP-14) in relation to missing teeth in the Polish population aged 20–79. This was a cross-sectional study carried out among 1112 randomly selected participants. The mean age was 48.72 and mean number of teeth was 20.12. Altogether, in the GOHAI, the percentage that gave a positive response to each question ranged from 3.3% to 48.0%; in the OHIP-14, these answers ranged from 2.4% to 25.1%. The GOHAI measure was statistically significant, with more grouping variables than the OHIP-14 measure. Both measures showed significant associations with gender, age, dry mouth, education, professional status, number of teeth, and upper and lower total dentures. We detected a significant relationship between oral health–related quality of life and the factors influencing the presence or absence of dentition. Missing teeth were statistically associated with GOHAI, OHIP-14, advanced age, self-reported dry mouth, lower education, higher Body Mass Index (BMI), lower professional status, diabetes, myocardial infraction, and total dentures in upper or/and lower jaws. However, edentulous individuals had two times higher risk of having an OHIP-14 score above the median. This suggests that oral health practitioners should work to prevent oral diseases that lead to tooth loss in their patients, starting from an early age.
A Systematic Review on Nerve-Related Adverse Effects following Mandibular Nerve Block Anesthesia
Authors: Aquilanti Luca , Mascitti Marco , Togni Lucrezia , Contaldo Maria , Rappelli Giorgio , Santarelli Andrea
Published: January 2022

Inferior alveolar nerve (IAN) block injections are commonly used in clinical practice, but they are not free from complications. The aim of the present systematic review is to assess the nerve-related adverse effects of IAN block anesthesia. A structured and systematic search was performed on the major electronic databases (PubMed, Cochrane Library, Web of Science, Scopus and CINAHL) for studies published in English until 30 September 2021. A total of 131 articles were identified through database searching using combinations of keywords. Fifteen papers were included and assessed for eligibility. Overall, nerve damage following an IAN block anesthesia injection is a rare occurrence, probably due to the direct nerve trauma of the needle, a neurotoxic effect of the used anesthetic solution and/or a combination of them. From a medico-legal point of view, a balanced discussion prior to nerve block anesthesia should be pursued in order to avoid patients’ reluctance to undergo necessary dental treatment due to the remote eventuality of nerve injury.
Creativity: The Effectiveness of Teacher–Student Conflict
Authors: Xie Ruoying , Jiang Jinzhang
Published: January 2022

This study examines the effectiveness of different types of teacher–student conflict in promoting students’ creativity in universities. Previous studies mainly focused on the negative effects of conflict; few examined its positive effects. Teacher–student conflict in university classes can take many forms; however, there are no clear definitions of the various types of such conflict. This study classified teacher–student conflict as understanding conflict, process conflict, and relationship conflict, and we used this classification to extend prior research by revealing the beneficial impacts of teacher–student conflict on students’ creativity. We empirically examined the relationship between teacher–student conflict and students’ creativity. The hypotheses were supported by using data from questionnaires completed by 2009 students at 17 American universities. We then conducted a hierarchical regression analysis of the data using structural equation modeling. The findings indicate that understanding conflict and process conflict had significant positive effects on students’ creativity, whereas relationship conflict had a significant negative effect on students’ creativity. This study thus revealed the positive effect of teacher–student conflict in university classes and suggests encouraging conflict (understanding conflict and process conflict) as a unique teaching method to stimulate students’ creativity.
A Literature Review of Wheelchair Transportation Safety Relevant to Automated Vehicles
Authors: Klinich Kathleen D. , Manary Miriam A. , Orton Nichole R. , Boyle Kyle J. , Hu Jingwen
Published: January 2022

This literature review summarizes wheelchair transportation safety, focusing on areas pertinent to designing automated vehicles (AVs) so they can accommodate people who remain seated in their wheelchairs for travel. In these situations, it is necessary to secure the wheelchair to the vehicle and provide occupant protection with a Wheelchair Tiedown and Occupant Restraint System (WTORS). For this population to use AVs, a WTORS must be crashworthy for use in smaller vehicles, able to be used independently, and adaptable for a wide range of wheelchair types. Currently available WTORS do not have these characteristics, but a universal docking interface geometry and prototype automatic seatbelt donning systems have been developed. In the absence of government regulations that address this situation, RESNA and ISO have developed voluntary industry standards to define design and performance criteria to achieve occupant protection levels for wheelchair-seated passengers that are similar to those provided by conventional vehicle seats.
Smoking Cessation Support for Pregnant Women Provided by English Stop Smoking Services and National Health Service Trusts: A Survey
Authors: Thomson Ross , Cooper Sue , Waldron John , Mamuzo Efe , McDaid Lisa , Emery Joanne , Phillips Lucy , Naughton Felix , Coleman Tim
Published: January 2022

Reducing smoking rates in pregnancy continues to be a public health priority. Given a recent UK government policy change resulting in The National Health Service (NHS) making a significant new contribution to cessation support in pregnancy in England, in addition to that of Local Authorities (LA), an up-to-date assessment of national support offered to pregnant women is needed. LA Stop Smoking Service (SSS) managers and representatives from maternity services in NHS Trusts were invited to participate in an online survey in autumn 2020. Topics included service configuration, staffing, practitioner consultations and treatments offered. The survey response rate was 66% (99/151) of LAs and 68% (95/140) of Trusts. Most LAs provided smoking support for pregnant smokers (78%), whereas under half (43%) of NHS Trusts did. Combination nicotine replacement therapy, i.e., a combination of a patch and short-acting product, was offered by LAs (92%) and Trusts (95%) and most commonly for 12 weeks duration, at 53% and 50%, respectively. Similar national online training was undertaken by those supporting women, with the majority undertaking the specialist pregnancy-specific module: LAs 60% and Trusts 79%. However, clinicians were reported to deliver specialist stop smoking support in over 50% of Trusts, whereas this was reported in only 16% of LAs. In England, both LA and NHS Trusts are currently delivering similar stop smoking support to pregnant women. Having nationally recognised treatment programmes and training allows for the delivery of consistent, evidence-based smoking cessation to pregnant women in different healthcare settings.
Strategies for Proteome-Wide Quantification of Glycosylation Macro- and Micro-Heterogeneity
Authors: Fang Pan , Ji Yanlong , Oellerich Thomas , Urlaub Henning , Pan Kuan-Ting
Published: January 2022

Protein glycosylation governs key physiological and pathological processes in human cells. Aberrant glycosylation is thus closely associated with disease progression. Mass spectrometry (MS)-based glycoproteomics has emerged as an indispensable tool for investigating glycosylation changes in biological samples with high sensitivity. Following rapid improvements in methodologies for reliable intact glycopeptide identification, site-specific quantification of glycopeptide macro- and micro-heterogeneity at the proteome scale has become an urgent need for exploring glycosylation regulations. Here, we summarize recent advances in N- and O-linked glycoproteomic quantification strategies and discuss their limitations. We further describe a strategy to propagate MS data for multilayered glycopeptide quantification, enabling a more comprehensive examination of global and site-specific glycosylation changes. Altogether, we show how quantitative glycoproteomics methods explore glycosylation regulation in human diseases and promote the discovery of biomarkers and therapeutic targets.
Genetic Interaction between Mfrp and Adipor1 Mutations Affect Retinal Disease Phenotypes
Authors: Gogna Navdeep , Weatherly Sonia , Zhao Fuxin , Collin Gayle B. , Pinkney Jai , Stone Lisa , Naggert Jürgen K. , Carter Gregory W. , Nishina Patsy M.
Published: January 2022

Adipor1tm1Dgen and Mfrprd6 mutant mice share similar eye disease characteristics. Previously, studies established a functional relationship of ADIPOR1 and MFRP proteins in maintaining retinal lipidome homeostasis and visual function. However, the independent and/or interactive contribution of both genes to similar disease phenotypes, including fundus spots, decreased axial length, and photoreceptor degeneration has yet to be examined. We performed a gene-interaction study where homozygous Adipor1tm1Dgen and Mfrprd6 mice were bred together and the resulting doubly heterozygous F1 offspring were intercrossed to produce 210 F2 progeny. Four-month-old mice from all nine genotypic combinations obtained in the F2 generation were assessed for white spots by fundus photo documentation, for axial length by caliper measurements, and for photoreceptor degeneration by histology. Two-way factorial ANOVA was performed to study individual as well as gene interaction effects on each phenotype. Here, we report the first observation of reduced axial length in Adipor1tmlDgen homozygotes. We show that while Adipor1 and Mfrp interact to affect spotting and degeneration, they act independently to control axial length, highlighting the complex functional association between these two genes. Further examination of the molecular basis of this interaction may help in uncovering mechanisms by which these genes perturb ocular homeostasis.
Phytomedicines to Target Hepatitis B Virus DNA Replication: Current Limitations and Future Approaches
Authors: Sadiea Rahila Zannat , Sultana Shahnaj , Chaki Bijan Mohon , Islam Tasnim , Dash Sharmy , Akter Sharmin , Islam Md Sayeedul , Kazi Taheruzzaman , Nagata Abir , Spagnuolo Rocco , Mancina Rosellina Margherita , Hossain Md Golzar
Published: January 2022

Hepatitis B virus infection (HBV) is one of the most common causes of hepatitis, and may lead to cirrhosis or hepatocellular carcinoma. According to the World Health Organization (WHO), approximately 296 million people worldwide are carriers of the hepatitis B virus. Various nucleos(t)ide analogs, which specifically suppress viral replication, are the main treatment agents for HBV infection. However, the development of drug-resistant HBV strains due to viral genomic mutations in genes encoding the polymerase protein is a major obstacle to HBV treatment. In addition, adverse effects can occur in patients treated with nucleos(t)ide analogs. Thus, alternative anti-HBV drugs of plant origin are being investigated as they exhibit excellent safety profiles and have few or no side effects. In this study, phytomedicines/phytochemicals exerting significant inhibitory effects on HBV by interfering with its replication were reviewed based on different compound groups. In addition, the chemical structures of these compounds were developed. This will facilitate their commercial synthesis and further investigation of the molecular mechanisms underlying their effects. The limitations of compounds previously screened for their anti-HBV effect, as well as future approaches to anti-HBV research, have also been discussed.
PD-L1 Expression in Monocytes Correlates with Bacterial Burden and Treatment Outcomes in Active Pulmonary Tuberculosis
Authors: Pan Sheng-Wei , Shu Chin-Chung , Huang Jhong-Ru , Lee Chang-Ching , Tseng Yen-Han , Hung Jung-Jyh , Hsu Po-Kuei , Chen Nien-Jung , Su Wei-Juin , Feng Jia-Yih , Chen Yuh-Min
Published: January 2022

The PD-1/PD-L1 pathway is critical in T cell biology; however, the role of the PD-1/PD-L1 pathway in clinical characteristics and treatment outcomes in pulmonary tuberculosis (PTB) patients is unclear. We prospectively enrolled PTB, latent TB infection (LTBI), and non-TB, non-LTBI subjects. The expression of PD-1/PD-L1 on peripheral blood mononuclear cells (PBMCs) was measured and correlated with clinical characteristics and treatment outcomes in PTB patients. Immunohistochemistry and immunofluorescence were used to visualize PD-1/PD-L1-expressing cells in lung tissues from PTB patients and from murine with heat-killed MTB (HK-MTB) treatment. A total of 76 PTB, 40 LTBI, and 28 non-TB, non-LTBI subjects were enrolled. The expression of PD-1 on CD4+ T cells and PD-L1 on CD14+ monocytes was significantly higher in PTB cases than non-TB subjects. PTB patients with sputum smear/culture unconversion displayed higher PD-L1 expression on monocytes. PD-L1-expressing macrophages were identified in lung tissue from PTB patients, and co-localized with macrophages in murine lung tissues. Mycobacterium tuberculosis (MTB) whole cell lysate/EsxA stimulation of human and mouse macrophages demonstrated increased PD-L1 expression. In conclusion, increased expression of PD-L1 on monocytes in PTB patients correlated with higher bacterial burden and worse treatment outcomes. The findings suggest the involvement of the PD-1/PD-L1 pathway in MTB-related immune responses.
Virtual Combinatorial Chemistry and Pharmacological Screening: A Short Guide to Drug Design
Authors: Suay-García Beatriz , Bueso-Bordils Jose I. , Falcó Antonio , Antón-Fos Gerardo M. , Alemán-López Pedro A.
Published: January 2022

Traditionally, drug development involved the individual synthesis and biological evaluation of hundreds to thousands of compounds with the intention of highlighting their biological activity, selectivity, and bioavailability, as well as their low toxicity. On average, this process of new drug development involved, in addition to high economic costs, a period of several years before hopefully finding a drug with suitable characteristics to drive its commercialization. Therefore, the chemical synthesis of new compounds became the limiting step in the process of searching for or optimizing leads for new drug development. This need for large chemical libraries led to the birth of high-throughput synthesis methods and combinatorial chemistry. Virtual combinatorial chemistry is based on the same principle as real chemistry—many different compounds can be generated from a few building blocks at once. The difference lies in its speed, as millions of compounds can be produced in a few seconds. On the other hand, many virtual screening methods, such as QSAR (Quantitative Sturcture-Activity Relationship), pharmacophore models, and molecular docking, have been developed to study these libraries. These models allow for the selection of molecules to be synthesized and tested with a high probability of success. The virtual combinatorial chemistry–virtual screening tandem has become a fundamental tool in the process of searching for and developing a drug, as it allows the process to be accelerated with extraordinary economic savings.
Interactions of Nanoparticles with Macrophages and Feasibility of Drug Delivery for Asthma
Authors: Kang Sung Hun , Shin Yoo Seob , Lee Dong-Hyun , Park Il Seok , Kim Sung Kyun , Ryu DongHun , Park YongKeun , Byun Soo-Hwan , Choi Jeong-Hee , Hong Seok Jin
Published: January 2022

Understanding the interaction between nanoparticles and immune cells is essential for the evaluation of nanotoxicity and development of nanomedicines. However, to date, there is little data on the membrane microstructure and biochemical changes in nanoparticle-loaded immune cells. In this study, we observed the microstructure of nanoparticle-loaded macrophages and changes in lipid droplets using holotomography analysis. Quantitatively analyzing the refractive index distribution of nanoparticle-loaded macrophages, we identified the interactions between nanoparticles and macrophages. The results showed that, when nanoparticles were phagocytized by macrophages, the number of lipid droplets and cell volume increased. The volume and mass of the lipid droplets slightly increased, owing to the absorption of nanoparticles. Meanwhile, the number of lipid droplets increased more conspicuously than the other factors. Furthermore, alveolar macrophages are involved in the development and progression of asthma. Studies have shown that macrophages play an essential role in the maintenance of asthma-related inflammation and tissue damage, suggesting that macrophage cells may be applied to asthma target delivery strategies. Therefore, we investigated the target delivery efficiency of gold nanoparticle-loaded macrophages at the biodistribution level, using an ovalbumin-induced asthma mouse model. Normal and severe asthma models were selected to determine the difference in the level of inflammation in the lung. Consequently, macrophages had increased mobility in models of severe asthma, compared to those of normal asthma disease. In this regard, the detection of observable differences in nanoparticle-loaded macrophages may be of primary interest, as an essential endpoint analysis for investigating nanomedical applications and immunotheragnostic strategies.
High Iron and Iron Household Protein Contents in Perineuronal Net-Ensheathed Neurons Ensure Energy Metabolism with Safe Iron Handling
Authors: Reinert Anja , Reinert Tilo , Arendt Thomas , Morawski Markus
Published: January 2022

A subpopulation of neurons is less vulnerable against iron-induced oxidative stress and neurodegeneration. A key feature of these neurons is a special extracellular matrix composition that forms a perineuronal net (PN). The PN has a high affinity to iron, which suggests an adapted iron sequestration and metabolism of the ensheathed neurons. Highly active, fast-firing neurons—which are often ensheathed by a PN—have a particular high metabolic demand, and therefore may have a higher need in iron. We hypothesize that PN-ensheathed neurons have a higher intracellular iron concentration and increased levels of iron proteins. Thus, analyses of cellular and regional iron and the iron proteins transferrin (Tf), Tf receptor 1 (TfR), ferritin H/L (FtH/FtL), metal transport protein 1 (MTP1 aka ferroportin), and divalent metal transporter 1 (DMT1) were performed on Wistar rats in the parietal cortex (PC), subiculum (SUB), red nucleus (RN), and substantia nigra (SNpr/SNpc). Neurons with a PN (PN+) have higher iron concentrations than neurons without a PN: PC 0.69 mM vs. 0.51 mM, SUB 0.84 mM vs. 0.69 mM, SN 0.71 mM vs. 0.63 mM (SNpr)/0.45 mM (SNpc). Intracellular Tf, TfR and MTP1 contents of PN+ neurons were consistently increased. The iron concentration of the PN itself is not increased. We also determined the percentage of PN+ neurons: PC 4%, SUB 5%, SNpr 45%, RN 86%. We conclude that PN+ neurons constitute a subpopulation of resilient pacemaker neurons characterized by a bustling iron metabolism and outstanding iron handling capabilities. These properties could contribute to the low vulnerability of PN+ neurons against iron-induced oxidative stress and degeneration.
A Model to Incorporate the bHLH Transcription Factor OsIRO3 within the Rice Iron Homeostasis Regulatory Network
Authors: Carey-Fung Oscar , O’Brien Martin , Beasley Jesse T. , Johnson Alexander A. T.
Published: January 2022

Iron (Fe) homeostasis in plants is governed by a complex network of regulatory elements and transcription factors (TFs), as both Fe toxicity and deficiency negatively impact plant growth and physiology. The Fe homeostasis network is well characterized in Arabidopsis thaliana and remains poorly understood in monocotyledon species such as rice (Oryza sativa L.). Recent investigation of the rice Fe homeostasis network revealed OsIRO3, a basic Helix–Loop–Helix (bHLH) TF as a putative negative regulator of genes involved in Fe uptake, transport, and storage. We employed CRISPR-Cas9 gene editing to target the OsIRO3 coding sequence and generate two independent T-DNA-free, loss-of-function iro3 mutants in rice cv. Nipponbare. The iro3 mutant plants had similar phenotype under nutrient-sufficient conditions and had stunted growth under Fe-deficient conditions, relative to a T-DNA free, wild-type control (WT). Under Fe deficiency, iro3 mutant shoots had reduced expression of Fe chelator biosynthetic genes (OsNAS1, OsNAS2, and OsNAAT1) and upregulated expression of an Fe transporter gene (OsYSL15), relative to WT shoots. We place our results in the context of the existing literature and generate a model describing the role of OsIRO3 in rice Fe homeostasis and reinforce the essential function of OsIRO3 in the rice Fe deficiency response.
MiR-29a Curbs Hepatocellular Carcinoma Incidence via Targeting of HIF-1α and ANGPT2
Authors: Huang Ying-Hsien , Lian Wei-Shiung , Wang Feng-Sheng , Wang Pei-Wen , Lin Hung-Yu , Tsai Ming-Chao , Yang Ya-Ling
Published: January 2022

A high-fat diet is responsible for hepatic fat accumulation that sustains chronic liver damage and increases the risks of steatosis and hepatocellular carcinoma (HCC). MicroRNA-29a (miR-29a), a key regulator of cellular behaviors, is present in anti-fibrosis and modulator tumorigenesis. However, the increased transparency of the correlation between miR-29a and the progression of human HCC is still further investigated. In this study, we predicted HIF-1α and ANGPT2 as regulators of HCC by the OncoMir cancer database and showed a strong positive correlation with HIF-1α and ANGPT2 gene expression in HCC patients. Mice fed the western diet (WD) while administered CCl4 for 25 weeks induced chronic liver damage and higher HCC incidence than without fed WD mice. HCC section staining revealed signaling upregulation in ki67, severe fibrosis, and steatosis in WD and CCl4 mice and detected Col3a1 gene expressions. HCC tissues significantly attenuated miR-29a but increased in HIF-1α, ANGPT2, Lox, Loxl2, and VEGFA expression. Luciferase activity analysis confirms that miR-29a specific binding 3′UTR of HIF-1α and ANGPT2 to repress expression. In summary, miR-29a control HIF-1α and ANGPT2 signaling in HCC formation. This study insight into a novel molecular pathway by which miR-29a targeting HIF-1α and ANGPT2 counteracts the incidence of HCC development.
Modernistic and Emerging Developments of Nanotechnology in Glioblastoma-Targeted Theranostic Applications
Authors: Lakshmi Buddolla Anantha , Kim Young-Joon
Published: January 2022

Brain tumors such as glioblastoma are typically associated with an unstoppable cell proliferation with aggressive infiltration behavior and a shortened life span. Though treatment options such as chemotherapy and radiotherapy are available in combating glioblastoma, satisfactory therapeutics are still not available due to the high impermeability of the blood–brain barrier. To address these concerns, recently, multifarious theranostics based on nanotechnology have been developed, which can deal with diagnosis and therapy together. The multifunctional nanomaterials find a strategic path against glioblastoma by adjoining novel thermal and magnetic therapy approaches. Their convenient combination of specific features such as real-time tracking, in-depth tissue penetration, drug-loading capacity, and contrasting performance is of great demand in the clinical investigation of glioblastoma. The potential benefits of nanomaterials including specificity, surface tunability, biodegradability, non-toxicity, ligand functionalization, and near-infrared (NIR) and photoacoustic (PA) imaging are sufficient in developing effective theranostics. This review discusses the recent developments in nanotechnology toward the diagnosis, drug delivery, and therapy regarding glioblastoma.
Development of Planar Illumination Strategies for Solving Mysteries in the Sub-Cellular Realm
Authors: Teranikar Tanveer , Lim Jessica , Ijaseun Toluwani , Lee Juhyun
Published: January 2022

Optical microscopy has vastly expanded the frontiers of structural and functional biology, due to the non-invasive probing of dynamic volumes in vivo. However, traditional widefield microscopy illuminating the entire field of view (FOV) is adversely affected by out-of-focus light scatter. Consequently, standard upright or inverted microscopes are inept in sampling diffraction-limited volumes smaller than the optical system’s point spread function (PSF). Over the last few decades, several planar and structured (sinusoidal) illumination modalities have offered unprecedented access to sub-cellular organelles and 4D (3D + time) image acquisition. Furthermore, these optical sectioning systems remain unaffected by the size of biological samples, providing high signal-to-noise (SNR) ratios for objective lenses (OLs) with long working distances (WDs). This review aims to guide biologists regarding planar illumination strategies, capable of harnessing sub-micron spatial resolution with a millimeter depth of penetration.
Activity and Function in Human Cells of the Evolutionary Conserved Exonuclease Polynucleotide Phosphorylase
Authors: Falchi Federica A. , Pizzoccheri Roberto , Briani Federica
Published: January 2022

Polynucleotide phosphorylase (PNPase) is a phosphorolytic RNA exonuclease highly conserved throughout evolution. Human PNPase (hPNPase) is located in mitochondria and is essential for mitochondrial function and homeostasis. Not surprisingly, mutations in the PNPT1 gene, encoding hPNPase, cause serious diseases. hPNPase has been implicated in a plethora of processes taking place in different cell compartments and involving other proteins, some of which physically interact with hPNPase. This paper reviews hPNPase RNA binding and catalytic activity in relation with the protein structure and in comparison, with the activity of bacterial PNPases. The functions ascribed to hPNPase in different cell compartments are discussed, highlighting the gaps that still need to be filled to understand the physiological role of this ancient protein in human cells.
Broad Specific Xyloglucan:Xyloglucosyl Transferases Are Formidable Players in the Re-Modelling of Plant Cell Wall Structures
Authors: Hrmova Maria , Stratilová Barbora , Stratilová Eva
Published: January 2022

Plant xyloglucan:xyloglucosyl transferases, known as xyloglucan endo-transglycosylases (XETs) are the key players that underlie plant cell wall dynamics and mechanics. These fundamental roles are central for the assembly and modifications of cell walls during embryogenesis, vegetative and reproductive growth, and adaptations to living environments under biotic and abiotic (environmental) stresses. XET enzymes (EC 2.4.1.207) have the β-sandwich architecture and the β-jelly-roll topology, and are classified in the glycoside hydrolase family 16 based on their evolutionary history. XET enzymes catalyse transglycosylation reactions with xyloglucan (XG)-derived and other than XG-derived donors and acceptors, and this poly-specificity originates from the structural plasticity and evolutionary diversification that has evolved through expansion and duplication. In phyletic groups, XETs form the gene families that are differentially expressed in organs and tissues in time- and space-dependent manners, and in response to environmental conditions. Here, we examine higher plant XET enzymes and dissect how their exclusively carbohydrate-linked transglycosylation catalytic function inter-connects complex plant cell wall components. Further, we discuss progress in technologies that advance the knowledge of plant cell walls and how this knowledge defines the roles of XETs. We construe that the broad specificity of the plant XETs underscores their roles in continuous cell wall restructuring and re-modelling.
Comparison of the Ecological Traits and Boring Densities of Aromia bungii (Faldermann, 1835) (Coleoptera: Cerambycidae) in Two Host Tree Species
Authors: Urano Tadahisa , Taki Hisatomo , Shoda-Kagaya Etsuko
Published: January 2022

We investigated the ecological traits of emerging adults and the boring density in Aromia bungii-infested flowering cherry (Cerasus × yedoensis ‘Somei-yoshino’) and peach (Prunus persica) trees to evaluate their suitability as food resources for A. bungii, and their vulnerability to infestation. The number of adults per m3 that emerged from P. persica was 10-times larger than from C. × yedoensis, and the numbers of emergence holes, entrance holes, and pupal chambers were also larger in P. persica logs. The lifetime fecundity of adults that emerged from P. persica was also higher. Elytral length, sex ratios, and adult lifespans did not differ between the two host trees. Our results indicate that peach trees provide more suitable conditions than do flowering cherry trees for A. bungii larvae. Although flowering cherry trees, primarily C. × yedoensis, which are currently grown as street or ornamental trees in Japan, have been more severely affected by A. bungii to date, the greater risk in the long term is to P. persica, an agricultural species in the main producing areas surrounding the Kanto region.
Fine Structure of the Visual System of Arge similis (Hymenoptera, Argidae)
Authors: Wen Chao , Pan Zijian , Liang Shiping , Shen Liming , Wen Xiujun , Wang Cai
Published: January 2022

External morphology and ultrastructure of the visual system of Arge similis (Vollenhoven, 1860) adults were investigated by light microscopy, scanning electron microscopy, and transmission electron microscopy. Each compound eye contains 2022 ± 89 (mean ± SE) facets in males and 2223 ± 52 facets in females. Arge similis has an apposition kind of compound eye composed of a cornea, a crystalline cone of four cone cells, and a centrally fused rhabdom made up of the rhabdomeres of eight large retinular cells. Each crystalline cone is surrounded by primary and secondary pigment cells with black spherical screening pigment granules measuring 0.60 ± 0.02 and 0.41 ± 0.01 μm in diameter, respectively. Based on our findings, the compound eye of A. similis can be expected to exhibit high adaptability to light intensity changes.
The Native Bees of Lolland (Denmark) Revisited after 100 Years: The Demise of the Specialists
Authors: Rasmussen Claus , Sydenham Markus Arne Kjær , Schmidt Hans Thomsen , Madsen Henning Bang
Published: January 2022

There is a global concern over insect declines, including both species loss and population declines. In particular, declines of species, such as bees that anchor trophic interactions and shoulder many of the essential ecosystem services, have been the focus of broad public concern. However, our understanding of what characterizes those species that are lost because of declines over long periods is limited by a scarcity of comparative studies. We here compare the species composition from a collection of bees sampled over two decades (2000–2019) from the island of Lolland in Denmark, with a collection also sampled over two decades (1900–1919), but a century ago by Jørgensen and his contemporaries. We further test if (1) the probability that bee species that were sampled a century ago are also found today depends on their degree of floral specialization; (2) and use information from pollen samples from bees from the historical records to assess if certain floral resources have been lost. In total, 203 species were recorded in the two periods, but less than half, 92 species, occurred in both sampling periods. A total of 174 species of bees were recorded from 1900–1919, and 121 species were recorded from 2000–2019, including 29 species not reported in 1900–1919. Notably, we report a reduction in the species composition among forage specialist bees from 26.4% to 15.7% of the bee fauna, a consistent and highly significant decline both when correcting for parasitic and social species. Pollen swabs from bees collected in the first period, 1900–1919, did not identify any plants that are not available today but documented a series of plants that were important to bees back then. These plants are still common today, such as Taraxacum and Salix. Our findings highlight the importance of local and historical faunistic studies, such as that of Jørgensen, who was a resident schoolteacher on the island of Lolland in southern Denmark, for documenting how changes over time affect the species composition in bee communities.
Factors Predicting the Success of Combined Orbital Decompression and Strabismus Surgery in Thyroid-Associated Orbitopathy
Authors: Hsieh Meng-Wei , Hsu Chih-Kang , Kuo Pao-Cheng , Chang Hsu-Chieh , Chen Yi-Hao , Chien Ke-Hung
Published: January 2022

To evaluate the safety and efficacy of orbital decompression combined with strabismus surgery in thyroid-associated orbitopathy (TAO) and identify factors leading to surgical success. A retrospective comparative case series was conducted on 52 patients who were treated with combined orbital decompression and strabismus surgery. Outcome measurements included perioperative Hertel exophthalmometry and strabismus measurements. Surgical success was defined as binocular single vision (BSV) in the primary and reading positions within 5 prism diopters (PDs). As a result, the average reduction in proptosis was 3.23 mm, with a mean preoperative Hertel measurement of 22.64 mm. Forty-four patients (84.6%) achieved the success criterion and composed the success group. In addition to sex and underlying hyperthyroidism, symmetry of orbitopathy, interocular exophthalmos difference of more than 2 mm, predominant esotropia type, mixed type strabismus, baseline horizontal deviations, baseline vertical deviations, and combination with one-wall decompression surgery were significantly different between the success and failure groups. All complications were mild and temporary. Orbital decompression combined with strabismus surgery produced satisfactory outcomes in selected patients with efficacy and safety. Symmetry between the two eyes with relatively simple strabismus and proptosis ensured surgical success. With experienced surgeons, advanced techniques, and selected patients, this method can serve as an alternative treatment option to minimize the number of surgeries, medical costs and recovery period.
Composite Hat Structure Design for Vehicle Safety: Potential Application to B-Pillar and Door Intrusion Beam
Authors: Abdulqadir Samer Fakhri , Tarlochan Faris
Published: January 2022

Regarding crashworthiness, many published works have focused on designing thin-walled structures for frontal collisions compared to side-impact collisions. This paper presents an experimental investigation and finite element modelling of a carbon-reinforced thin-walled top-hat section subjected to quasi-static and dynamic transverse bending loads at different impact speeds. The top-hat sections and their closure assembly plates were made of MTM44 prepreg carbon. The specimens were manufactured by vacuum bagging. Dynamic work was performed to validate the results obtained from the finite element analysis (FEA). The predicted results are in good agreement with the experimental results. The study also showed that the peak load and energy absorption owing to dynamic loading were higher than those under static loading. In the four-point bend analysis, the stacking sequence affected the energy absorption capabilities by 15–30%. In addition, the distance between the indenters in the four-point analysis also affected the energy absorption by 10% for the same impact condition, where a larger distance promoted higher energy absorption. The study also demonstrated that a top-hat shaped thin-walled structure is suitable for deep intrusion beams in vehicle doors for side-impact crashworthiness applications.