bluuebunny/crossref_metadata_2025 huggingface dataset:
Exploiting the Generative Adversarial Network Approach to Create a Synthetic Topography Corneal Image
Authors: Jameel Samer Kais , Aydin Sezgin , Ghaeb Nebras H. , Majidpour Jafar , Rashid Tarik A. , Salih Sinan Q. , JosephNg Poh Soon
Published: December 2022

Corneal diseases are the most common eye disorders. Deep learning techniques are used to perform automated diagnoses of cornea. Deep learning networks require large-scale annotated datasets, which is conceded as a weakness of deep learning. In this work, a method for synthesizing medical images using conditional generative adversarial networks (CGANs), is presented. It also illustrates how produced medical images may be utilized to enrich medical data, improve clinical decisions, and boost the performance of the conventional neural network (CNN) for medical image diagnosis. The study includes using corneal topography captured using a Pentacam device from patients with corneal diseases. The dataset contained 3448 different corneal images. Furthermore, it shows how an unbalanced dataset affects the performance of classifiers, where the data are balanced using the resampling approach. Finally, the results obtained from CNN networks trained on the balanced dataset are compared to those obtained from CNN networks trained on the imbalanced dataset. For performance, the system estimated the diagnosis accuracy, precision, and F1-score metrics. Lastly, some generated images were shown to an expert for evaluation and to see how well experts could identify the type of image and its condition. The expert recognized the image as useful for medical diagnosis and for determining the severity class according to the shape and values, by generating images based on real cases that could be used as new different stages of illness between healthy and unhealthy patients.
Unconventional Myosins from Caenorhabditis elegans as a Probe to Study Human Orthologues
Authors: Johnson Chloe A , Behbehani Ranya , Buss Folma
Published: December 2022

Unconventional myosins are a superfamily of actin-based motor proteins that perform a number of roles in fundamental cellular processes, including (but not limited to) intracellular trafficking, cell motility, endocytosis, exocytosis and cytokinesis. 40 myosins genes have been identified in humans, which belong to different 12 classes based on their domain structure and organisation. These genes are widely expressed in different tissues, and mutations leading to loss of function are associated with a wide variety of pathologies while over-expression often results in cancer. Caenorhabditis elegans (C. elegans) is a small, free-living, non-parasitic nematode. ~38% of the genome of C. elegans has predicted orthologues in the human genome, making it a valuable tool to study the function of human counterparts and human diseases. To date, 8 unconventional myosin genes have been identified in the nematode, from 6 different classes with high homology to human paralogues. The hum-1 and hum-5 (heavy chain of an unconventional myosin) genes encode myosin of class I, hum-2 of class V, hum-3 and hum-8 of class VI, hum-6 of class VII and hum-7 of class IX. The hum-4 gene encodes a high molecular mass myosin (307 kDa) that is one of the most highly divergent myosins and is a member of class XII. Mutations in many of the human orthologues are lethal, indicating their essential properties. However, a functional characterisation for many of these genes in C. elegans has not yet been performed. This article reviews the current knowledge of unconventional myosin genes in C. elegans and explores the potential use of the nematode to study the function and regulation of myosin motors to provide valuable insights into their role in diseases.
Modulation of Anionic Lipid Bilayers by Specific Interplay of Protons and Calcium Ions
Authors: Abhinav  , Jurkiewicz Piotr , Hof Martin , Allolio Christoph , Sýkora Jan
Published: December 2022

Biomembranes, important building blocks of living organisms, are often exposed to large local fluctuations of pH and ionic strength. To capture changes in the membrane organization under such harsh conditions, we investigated the mobility and hydration of zwitterionic and anionic lipid bilayers upon elevated H3O+ and Ca2+ content by the time-dependent fluorescence shift (TDFS) technique. While the zwitterionic bilayers remain inert to lower pH and increased calcium concentrations, anionic membranes are responsive. Specifically, both bilayers enriched in phosphatidylserine (PS) and phosphatidylglycerol (PG) become dehydrated and rigidified at pH 4.0 compared to at pH 7.0. However, their reaction to the gradual Ca2+ increase in the acidic environment differs. While the PG bilayers exhibit strong rehydration and mild loosening of the carbonyl region, restoring membrane properties to those observed at pH 7.0, the PS bilayers remain dehydrated with minor bilayer stiffening. Molecular dynamics (MD) simulations support the strong binding of H3O+ to both PS and PG. Compared to PS, PG exhibits a weaker binding of Ca2+ also at a low pH.
Effects of the Myokine Irisin on Stromal Cells from Swine Adipose Tissue
Authors: Basini Giuseppina , Bussolati Simona , Grolli Stefano , Berni Priscilla , Di Lecce Rosanna , Grasselli Francesca
Published: December 2022

Irisin is a hormone able to reproduce some of the positive effects of physical activity and diet. Recently, we demonstrated the presence of Irisin at the ovarian level as a potential physiological regulator of follicular function. Adipose tissue is crucial for reproductive function through its metabolic activity and the production of adipokines. At present, the exact nature of adipocyte precursors is still under debate, but an important role has been assigned to the population of adipose tissue mesenchymal stromal cells (ASCs) of perivascular origin. It should be noted that, when appropriately stimulated, ASCs can differentiate into preadipocytes and, subsequently, adipocytes. Therefore, this present study was undertaken to explore the potential effect of Irisin on ASCs, known for their high differentiative potential. Since Irisin expression in ASCs was confirmed by PCR, we tested its potential effects on the main functional activities of these cells, including proliferation (BrdU uptake); metabolic activity (ATP production); redox status, evaluated as the generation of free molecules such as superoxide anion and nitric oxide; and scavenger activities, assessed as both enzymatic (superoxide dismutase) and non-enzymatic antioxidant power. Moreover, we tested the effect of Irisin on ASCs adipogenic differentiation. BrdU uptake was significantly (p < 0.001) inhibited by Irisin, while ATP production was significantly (p < 0.05) increased. Both superoxide anion and nitric oxide generation were significantly increased (p < 0.001) by Irisin, while scavenger activity was significantly reduced (p < 0.05). Irisin was found to significantly (p < 0.05) inhibit ASCs adipogenic differentiation. Taken together, the present results suggest a potential local role of Irisin in the regulation of adipose tissue function.
Concomitant Administration of Red Ginseng Extract with Lactic Acid Bacteria Increases the Plasma Concentration of Deglycosylated Ginsenosides in Healthy Human Subjects
Authors: Jeon Ji-Hyeon , Park Jin-Hyang , Jeon So Yeon , Pang Minyeong , Choi Min-Koo , Song Im-Sook
Published: December 2022

With the increased frequency of red ginseng extract (RGE) and lactic acid bacteria (LAB) co-administration, we aimed to investigate the interactions between RGE and LAB with regard to in vitro and in vivo deglycosylation metabolism and the pharmacokinetics of ginsenosides. As a proof-of-concept study, five healthy humans were administered RGE (104.1 mg of total ginsenosides/day) with or without co-administration of LAB (2 g, 1 billion CFU/day) for 2 weeks, and the plasma concentrations of ginsenosides in human plasma were monitored. The plasma exposure to compound K (CK), ginsenoside Rh2 (GRh2), protopanaxadiol (PPD), and protopanaxatriol (PPT) in the concomitant administration RGE and LAB groups increased by 2.7-, 2.1-, 1.6-, and 3.5-fold, respectively, compared to those in the RGE administration group, without a significant change in Tmax. The plasma concentrations of GRb1, GRb2, and GRc remained unchanged, whereas the AUC values of GRd and GRg3 significantly decreased in the concomitant administration RGE and LAB groups. To understand the underlying mechanism, the in vitro metabolic activity of ginsenosides was measured during the fermentation of RGE or individual ginsenosides in the presence of LAB for 1 week. Consistent with the in vivo results, co-incubation with RGE and LAB significantly increased the formation rate of GRh2, CK, PPD, and PPT. These results may be attributed to the facilitated deglycosylation of GRd and GRg3 and the increased production of GRh2, CK, PPD, and PPT by the co-administration of LAB and RGE. In conclusion, LAB supplementation increased the plasma concentrations of deglycosylated ginsenosides, such as GRh2, CK, PPD, and PPT, through facilitated deglycosylation metabolism of ginsenosides in the intestine.
Phenolipids, Amphipilic Phenolic Antioxidants with Modified Properties and Their Spectrum of Applications in Development: A Review
Authors: Arzola-Rodríguez Silvia Ivonne , Muñoz-Castellanos Laila-Nayzzel , López-Camarillo César , Salas Erika
Published: December 2022

Polyphenols, as secondary metabolites from plants, possess a natural antioxidant capacity and biological activities attributed to their chemical and structural characteristics. Due to their mostly polar character, polyphenols present a low solubility in less polar environments or hydrophobic matrices. However, in order to make polyphenols able to incorporate in oils and fats, a transformation strategy is necessary. For the above, the functionalization of polyphenols through chemical or enzymatic lipophilization has allowed the synthesis of phenolipids. These are amphipilic molecules that preserve the natural phenolic core to which an aliphatic motif is attached by esterification or transesterification reactions. The length of the aliphatic chain in phenolipids allows them to interact with different systems (such as emulsions, oily molecules, micelles and cellular membranes), which would favor their use in processed foods, as vehicles for drugs, antimicrobial agents, antioxidants in the cosmetic industry and even in the treatment of degenerative diseases related to oxidative stress
Elevated Expression and Activation of GPR15 in Immune Cells in Graves’ Disease
Authors: Zhao Jing , Liu Xuerong , Xu Jianbin , Fang Yudie , Du Peng , Gao Chaoqun , Cai Tiantian , Gu Zhaohua , Qin Qiu , Zhang Jin’an
Published: December 2022

GPR15 plays an important role in lymphocyte homing and is a key immune molecule to maintain organ immune homeostasis. Yet, no study on the association between GPR15 and Graves’ disease (GD) is available. In this study, we systematically investigated the expression of GPR15 in different types of immune cells and different tissues of GD patients. We found that the expressions of GPR15 and GPR15L in peripheral blood of GD patients were increased compared with those in healthy controls. A flow cytometry analysis showed that GPR15 positive cells were mainly CD14+ monocytes and CD56+ natural killer cells (NK cells) of innate immunity, T helper cells and cytotoxic T cells of adaptive immunity. We also found that the expressions of GPR15 and GPR15L in the PBMC of GD patients were positively correlated with the Tfh-specific cytokines IL21 and IL4. In addition, immunohistochemistry showed that the level of GPR15 in thyroid tissue of GD patients was higher than that of the control group. Our results demonstrate for the first time that GPR15 is highly expressed in various immune cells in GD patients, suggesting that GPR15-GPR15L is associated with the activation and infiltration of proinflammatory immune cells in the thyroid tissue of GD patients.
Construction Technologies and Conservation Strategies for the Bell Tower of Former Nanking University (Nanjing, China)—A Case Study of a Typical Architectural Heritage of the American Church School in the Late 19th Century
Authors: Wang Hechi , Hu Zhanfang , Huang Yanyan , Chen Meng , Hu Linhua , Zhou Qi
Published: December 2022

The bell tower of Nanking University, built in 1888, is a milestone of modern educational architecture in Nanjing. It fully introduced Western architectural concepts and is an important historical witness to the cultural exchange between China and the West. At present, there are few studies on the conservation and reuse of the building, so the authors conducted the project. In this paper, we conducted research by combining qualitative and quantitative methods. The qualitative analysis includes analyses of historical maps, photos, text, surveys, mapping of buildings, and a comparative analysis. The quantitative analysis includes statistics on the distribution of the urban school architectural heritage, the number of buildings at Nanking University, and China’s school building design standards. Through the historical research and value analysis of the building, we explore its real information from the aspects of architectural style, space, structure, material, and construction technology, and propose a reasonable conservation method. According to the current urban planning policy and architectural heritage data, the functional upgrading and renovation of the building will be carried out. This paper explores authenticity and integrity-based restoration methods, as well as the adaptive-reuse strategy for China’s architectural heritage, which can provide a paradigm for regional development and historic campus renewal.
Assessing the Impacts of Carbon Tax and Improved Energy Efficiency on the Construction Industry: Based on CGE Model
Authors: Du Qiang , Dong Yanan , Li Jingtao , Zhao Yuelin , Bai Libiao
Published: December 2022

The rapid development of energy consumption and carbon emissions in the construction industry poses an enormous and negative challenge for China’s energy and environment. While maintaining moderate economic growth, it is particularly important to realize energy conservation and carbon reduction. Carbon tax policy, a direct tool to reduce carbon emissions, can effectively alleviate the environmental issues caused by construction activities. However, relying solely on a single method is insufficient to handle the complicated circumstances of China’s construction industry. This study explores the influence of carbon tax on the construction industry through adjustments to tax rates via developing a Computable General Equilibrium (CGE) model. Then, it analyzes how the carbon tax affects the economic and environmental variables by designing scenarios for recycling tax revenue and improved energy efficiency. The results indicate that the carbon tax rate of 40 RMB/t-CO2 is the most appropriate. At this tax level, the negative impacts of energy demand and emissions reduction on macroeconomy and construction industry are minimized. It was also determined that carbon tax revenue recycling to households and sectoral investment will realize the “weak double dividend” effect on the construction industry. Furthermore, improving energy efficiency in the construction industry will demonstrate the additional advantages of carbon tax. This study serves as a theoretical foundation for the Chinese government to develop various energy strategies to achieve low-carbon development in the construction industry.
Effect of Construction Errors in Cable Forces of Single-Story Orthogonal Cable Network Structures Based on GA-BPNN
Authors: Wang Zeqiang , Shi Guoliang , Liu Zhansheng , Mo Yanchi , Si Bo , Hu Yang , Wang Yongliang
Published: December 2022

The construction process of cable net structure is complicated, which leads to the strong randomness of construction errors. The safety state of the cable net structure is very sensitive to construction errors. Obtaining the coupling relationship between construction errors and cable force response efficiently and accurately is critical to developing the construction technique of cable structures. This paper proposed an analysis method based on a genetic algorithm optimized back propagation neural network (GA-BPNN) to judge the influence of construction error on the cable force of single-layer orthogonal cable network structures. Taking the speed skating stadium of the 2022 Winter Olympic Games as the research object, this paper analyzed the structure form of the venue. According to the characteristics of cable network structure and GA-BPNN calculation, the principle of construction error analysis was put forward. The influence of construction errors of load-bearing cables and stable cables on cable force response was analyzed. The influence degree of different component errors on structural cable forces was obtained, and the most unfavorable key components were obtained. For the key components, the influence trend of different construction errors on cable force was analyzed, and the fitting formula was given. Driven by GA-BPNN, it can realize the analysis of structural and mechanical responses under the action of multi-type, multi-component, and multi-combination construction errors. The results show that the research method efficiently and accurately obtains the performance law of structural cable force under the influence of construction error, effectively predicts the influencing factors of the structural safety risk, and effectively avoids structural safety accidents caused by construction error. The construction errors analysis method based on GA-BPNN proposed in this paper can provide a reference for similar structural analysis and application.
Evaluating the Effects of Different Improvement Strategies for the Outdoor Thermal Environment at a University Campus in the Summer: A Case Study in Northern China
Authors: Yang Lina , Liu Jiying , Zhu Shengwei
Published: December 2022

A lack of consideration of outdoor spaces of universities has resulted in lower outdoor thermal comfort in summer. This study investigates the thermal comfort of outdoor spaces of a university in summer and proposes the model’s accuracy and optimization strategies to improve the outdoor thermal environment, including vegetation greening, building morphology, and surface albedo. The ENVI-met program was used for the simulation. The measured data were utilized to verify the accuracy of the simulation model. The typical meteorological year data were applied as the inlet boundary condition of the optimized case. The simulation results show that vegetation greening has the most significant effect on improving the outdoor thermal environment. At a greening rate of 45%, the air temperature (Ta), mean radiant temperature (Tmrt), and physiological equivalent temperature (PET) in the study area were 3.2 °C, 14.4 °C, and 6.9 °C lower, respectively, than that in the base case. In areas shaded by building, the Ta, Tmrt, and PET were 2 °C, 8.7 °C, and 5.5 °C lower, respectively, than that in the base case. Increasing the height of buildings did not significantly improve thermal comfort when the height-to-width ratio (H/W) exceeded 1.0. Increasing the ground albedo from 0.2 (base case) to 0.6 can reduce the Ta by 1.44 °C but increase the Tmrt by 3.7 °C and the PET by 4.3 °C. These findings can be used by urban planners to develop sustainable cities and improve thermal comfort on university campuses.
Wind Tunnel Investigation of Twisted Wind Effect on a Typical Super-Tall Building
Authors: Yan Bowen , Li Yanan , Li Xiao , Zhou Xuhong , Wei Min , Yang Qingshan , Zhou Xu
Published: December 2022

This paper investigates the twisted wind effect on a typical super-tall building (500-m-tall square prism) by conducting pressure model wind tunnel tests. Two twisted wind fields (TWFs) with maximum yaw angles of approximately 30° and 20°, respectively, near the ground level were generated in the wind tunnel using a guide vane system, and the test results of wind pressure and wind load in TWFs were compared with those obtained in conventional wind fields (CWFs) with constant wind direction along the vertical axis. In particular, the distribution of extreme cladding pressure as well as the correlation and coherence of local wind loads are discussed in detail. It was observed that the mechanism of the structural dynamic responses, such as the vortex shedding, is greatly affected by TWFs. Both the distributions of mean and extreme cladding pressures in TWFs significantly differ from those in CWFs, especially on the windward and side facades. However, in terms of the amplitudes, the extreme wind pressure and the maximum wind load in TWFs do not noticeably exceed those in CWFs. This study aims to provide useful information for the wind-resistant design of future tall buildings.
A Special Type of Multifunctional Stadiums: Great Forest Stadium in Debrecen (Hungary)
Authors: Kozma Gábor , Czimre Klára , Bács Bence András , Ilieş Alexandru , Pfau Christa Sára , Bulz Gheorghe Codruț , Bács Zoltán
Published: December 2022

As a result of the stadium construction wave recently observed in North America and Europe, the question of the cost-effective operation of these facilities entered the foreground. Formerly, researchers advocated that these sports facilities had no significant positive economic impact, and no considerable increasing effect can be observed in terms of workplaces, personal incomes, or local tax revenues. In recent years, however, many researchers attribute a serious economic impact to particular facilities, including the so-called multifunctional stadiums, which are used for purposes other than one particular sports activity. The aim of our study, after summarising the factors leading to the establishment of multifunctional stadiums and the most important characteristics of the completed facilities, is to demonstrate the various utilization possibilities through the case study of the Great Forest Stadium in Debrecen. As an outcome of the research, it may be concluded that the facility has all features (conference room, catering unit, own shop, etc.) that are typical for medium-sized stadiums. Besides, the services related to health sciences constitute unique elements that can be explained by the activities of the higher education institution of the city, the University of Debrecen, which considers the Third Mission activities as particularly important.
Mechanical Properties and Absorption of High-Strength Fiber-Reinforced Concrete (HSFRC) with Sustainable Natural Fibers
Authors: Hasan Muttaqin , Saidi Taufiq , Jamil Muhammad , Amalia Zahra , Mubarak Azzaki
Published: December 2022

This study aimed to determine the mechanical properties and absorption of high-strength fiber-reinforced concrete (HSFRC), using sustainable natural fibers. In this analysis, two types of fibers were used, namely, ramie and abaca. Two different HSFRC mixtures were also designed, where one composition emphasized ordinary Portland cement (OPC) as a binder, and the other prioritizing calcined diatomaceous earth (CDE) as a mineral additive to replace 10% weight of OPC. Furthermore, ramie and abaca fibers were separately added to the mixtures at three different volumetric contents. Based on the results, the addition of these fibers in the concrete mixtures improved the mechanical properties of HSFRC. The improvements of compressive strength, splitting tensile strength, and flexural strength, due to the addition of ramie fiber were 18%, 17.3%, and 31.8%, respectively, while those for the addition of abaca fiber were 11.8%, 17.2%, and 38.1%, respectively. This indicated that the fibers were capable of being used as alternative materials for sustainable concrete production. The effects of ramie and abaca fibers on the absorption of HSFRC were also not significant, and their presence for the same amount of superplasticizer reduced the flow speed of fresh reinforced concrete mixtures.
Retrofitting of Shear Compression Failure-Critic Short Columns with a New Technique
Authors: Bedirhanoglu Idris
Published: December 2022

One of the reasons that cause the collapse of buildings is deficient short columns, which need to be retrofitted to prevent the collapse of the building in a potential earthquake. External reinforced concrete (RC), steel plates, and fiber-reinforced polymer (FRP) jacketing are standard retrofitting methods to retrofit columns to increase their shear capacity. However, in compression shear failure, the effectiveness of steel and FRP jacketing is quite limited due to the premature buckling of the FRP and steel material. On the other hand, RC jacketing is not practical because it requires more labor and covers more architectural places. Thus, the main motivation of this study is to present the effectiveness of a new method to retrofit short columns, including those with dominated shear compression failure. For this purpose, HSPRCC (high-performance steel plate-reinforced cementitious composite) was adapted to retrofit such short columns. This method is a combination of high-performance concrete and perforated steel plates. Short-column specimens representing existing RC buildings were retrofitted using the HSPRCC and tested. Perforated steel plates anchored to the specimen by steel bolts and repair mortar are used as a matrix. The retrofitted specimens were found to exhibit much better performance both in terms of shear strength and deformation capacity. It was also observed that the retrofitting method is effective in contributing to increasing the compression shear capacity.
Tensile Behavior of Basalt-Fiber-Grid-Reinforced Mortar before and after Exposure to Elevated Temperature
Authors: Zhang Xiaofei , He Weidong , Zhang Yongwang , Chen Cheng , Wu Xun
Published: December 2022

This paper presents an experimental study on the tensile behavior of basalt-fiber-grid-reinforced mortar (BGRM) before and after exposure to an elevated temperature of 300 °C considering the effect of fiber grid type, short polyvinyl alcohol fiber (PVA), and high-temperature exposure time. The experimental results show that the mortar plates reinforced with woven textile T25 and fiber-reinforced polymer (FRP) grid G50 exhibited more pronounced strain-hardening behavior. The highest peak stress was obtained for the T25-reinforced plate, which was 85% and 32% higher than that of the T5- and G50-reinforced plates, respectively. Meanwhile, the bridging effect of PVA fibers in mortar can improve the tensile properties. As the high-temperature exposure time increased, the cracking and peak stress of BGRM decreased significantly. Especially for the T5-reinforced plate after exposure to elevated temperature for 2 h, the cracking and peak stress decreased by 60.5% and 38%, respectively. The positive effect of short PVA fibers on the tensile properties of the BGRM became obsolete owing to the melting of short fibers at high temperature. Furthermore, an exponential strength degradation model related to high-temperature exposure time was proposed.
Clinical and Cytogenetic Characterization of Early and Late Relapses in Patients Allografted for Myeloid Neoplasms with a Myelodysplastic Component
Authors: Platte Victoria , Bergmann Anika , Hildebrandt Barbara , Wieczorek Dagmar , Schuler Esther , Germing Ulrich , Kaivers Jennifer , Haas Rainer , Kobbe Guido , Schroeder Thomas , Rautenberg Christina
Published: December 2022

An improved understanding of relapse kinetics is required to optimize detection and treatment strategies for the post-transplant relapse of myeloid neoplasms. Therefore, we retrospectively analyzed data from 91 patients allografted for MDS (n = 54), AML-MRC (n = 29) and chronic myelomonocytic leukemia (CMML, n = 8), who relapsed after transplant. Patients with early (<12 months, n = 56) and late relapse (>12 months, n = 35) were compared regarding patient-, disease- and transplant-related factors, including karyotype analyses at diagnosis and relapse. After a median follow-up of 17.4 months after relapse, late relapses showed improved outcomes compared with early relapses (2-yr OS 67% vs. 32%, p = 0.0048). Comparing frequency of distinct patient-, disease- and transplant-related factors among early and late relapses, complex karyotype (p = 0.0004) and unfavorable disease risk at diagnosis (p = 0.0008) as well as clonal evolution at relapse (p = 0.03) were more common in early than in late relapses. Furthermore, patients receiving transplant without prior cytoreduction or in complete remission were more frequently present in the group of late relapses. These data suggest that cytogenetics rather than disease burden at diagnosis and transplant-related factors determine the timepoint of post-transplant relapse and that upfront transplantation may be favored in order to delay relapse.
High-Dose-Rate Brachytherapy as an Organ-Sparing Treatment for Early Penile Cancer
Authors: Pohanková Denisa , Sirák Igor , Vošmik Milan , Kašaová Linda , Grepl Jakub , Paluska Petr , Holub Lukáš , Špaček Jiří , Hodek Miroslav , Kopeček Martin , Petera Jiří
Published: December 2022

Background: Low-dose-rate brachytherapy is an effective organ-sparing treatment for patients with early-stage penile cancer. However, only limited data are available on the role of high-dose-rate brachytherapy (HDR-BT) in this clinical setting. Methods: Between 2002 and 2020, 31 patients with early penile cancer were treated at our center with interstitial HDR BT at a dose of 18 × 3 Gy twice daily. A breast brachytherapy template was used for the fixation of stainless hollow needles. Results: The median follow-up was 117.5 months (range, 5–210). Eight patients (25.8%) developed a recurrence; of these, seven were salvaged by partial amputation. Six patients died of internal comorbidities or a second cancer. The probability of local control at 5 and 10 years was 80.7% (95% CI: 63.7–97.7%) and 68.3% (95% CI: 44.0–92.6%), respectively. Cause-specific survival was 100%. Only one case of radiation-induced necrosis was observed. The probability of penile sparing at 5 and 10 years was 80.6% (95% CI: 63.45–97.7%) and 62.1% (95% CI: 34.8–89.4%), respectively. Conclusions: These results show that HDR-BT for penile cancer can achieve results comparable to LDR-BT with organ sparing. Despite the relatively large patient cohort—the second largest reported to date in this clinical setting—prospective data from larger samples are needed to confirm the role of HDR-BT in penile cancer.
Central Nervous System Metastasis in Neuroblastoma: From Three Decades Clinical Experience to New Considerations in the Immunotherapy Era
Authors: Mastronuzzi Angela , Colafati Giovanna Stefania , Carai Andrea , D’Egidio Maria , Fabozzi Francesco , Del Bufalo Francesca , Villani Maria Felicia , Del Baldo Giada , Vennarini Sabina , Canino Costanza , Di Giannatale Angela , Tomà Paolo , Garganese Maria Carmen , De Ioris Maria Antonietta
Published: December 2022

Central nervous system (CNS) metastatic spread in neuroblastoma (NB) is rare and occurs more often at relapse/progression. We report on CNS involvement in high risk (HR) NB over 25 years. For this retrospective study, we reviewed the CNS imaging of all the patients treated at Bambino Gesù Children Hospital from 1 July 1996 to 30 June 2022. A total of 128 patients with HR NB were diagnosed over 26 years. Out of 128 patients, CNS metastatic spread occurred in 6 patients: 3 patients presented a metastatic spread at diagnosis, while in 3 patients, CNS was involved at relapse. Overall, the rate of occurrence of CNS spread is 4.7% with the same distribution at diagnosis and at relapse, namely 2.3%. Interestingly, CNS spread at diagnosis was observed only before 2012, whereas CNS was observed at relapse only after 2012, in the immunotherapy era. CNS metastases presented similar imaging features at diagnosis and at relapse, with a peculiar hemorrhagic aspect and mainly hemispheric localization in patients with bone skull involvement at the time of diagnosis. The outcome is dismal, and 3 out of 6 patients died for progressive disease.
Population-Based Temporal Trends and Ethnic Disparity in Cervical Cancer Mortality in South Africa (1999–2018): A Join Point and Age–Period–Cohort Regression Analyses
Authors: Olorunfemi Gbenga , Libhaber Elena , Ezechi Oliver , Musenge Eustasius
Published: December 2022

Cervical cancer is one of the leading causes of cancer deaths among women in low- and middle-income countries such as South Africa. The current impact of national cervical cancer control and sexual and reproductive health interventions in South Africa reduce its burden. The aim of this study was to assess the trends in cervical cancer mortality and its relation to breast and gynaecological cancers in South Africa from 1999 to 2018. We conducted joinpoint regression analyses of the trends in crude and age-standardised mortality rates (ASMR) for cervical cancer mortality in South Africa from 1999 to 2018. An age–period–cohort regression analysis was also conducted to determine the impact of age, period, and cohort on cervical cancer mortality trends. Analyses were stratified by ethnicity. Cervical cancer (n = 59,190, 43.92%, 95% CI: 43.65–44.18%) was responsible for about 43.9% of breast and gynecological cancer deaths. The mortality rate of cervical cancer (from 11.7 to 14.08 per 100,000) increased at about 0.9% per annum (Average Annual Percent Change (AAPC): 0.9% (AAPC: 0.9%, p-value < 0.001)), and young women aged 25 to 49 years (AAPC: 1.2–3.5%, p-value < 0.001) had increased rates. The risk of cervical cancer mortality increased among successive birth cohorts. In 2018, cervical cancer mortality rate among Blacks (16.74 per 100,000 women) was about twice the rates among Coloureds (8.53 deaths per 100,000 women) and approximately four-fold among Indians/Asians (4.16 deaths per 100,000 women), and Whites (3.06 deaths per 100,000 women). Cervical cancer control efforts should be enhanced in South Africa and targeted at ethnic difference, age, period, and cohort effects.
Therapeutic Target Identification and Inhibitor Screening against Riboflavin Synthase of Colorectal Cancer Associated Fusobacterium nucleatum
Authors: Alturki Norah A. , Mashraqi Mutaib M. , Jalal Khurshid , Khan Kanwal , Basharat Zarrin , Alzamami Ahmad
Published: December 2022

Colorectal cancer (CRC) ranks third among all cancers in terms of prevalence. There is growing evidence that gut microbiota has a role in the development of colorectal cancer. Fusobacterium nucleatum is overrepresented in the gastrointestinal tract and tumor microenvironment of patients with CRC. This suggests the role of F. nucleatum as a potential risk factor in the development of CRC. Hence, we aimed to explore whole genomes of F. nucleatum strains related to CRC to predict potential therapeutic markers through a pan-genome integrated subtractive genomics approach. In the current study, we identified 538 proteins as essential for F. nucleatum survival, 209 non-homologous to a human host, and 12 as drug targets. Eventually, riboflavin synthase (RiS) was selected as a therapeutic target for further processing. Three different inhibitor libraries of lead-like natural products, i.e., cyanobactins (n = 237), streptomycins (n = 607), and marine bacterial secondary metabolites (n = 1226) were screened against it. After the structure-based study, three compounds, i.e., CMNPD3609 (−7.63) > Malyngamide V (−7.03) > ZINC06804365 (−7.01) were prioritized as potential inhibitors of F. nucleatum. Additionally, the stability and flexibility of these compounds bound to RiS were determined via a molecular dynamics simulation of 50 ns. Results revealed the stability of these compounds within the binding pocket, after 5 ns. ADMET profiling showed compounds as drug-like, non-permeable to the blood brain barrier, non-toxic, and HIA permeable. Pan-genomics mediated drug target identification and the virtual screening of inhibitors is the preliminary step towards inhibition of this pathogenic oncobacterium and we suggest mouse model experiments to validate our findings.
Surgical Aspects of Intrahepatic Cholangiocarcinoma
Authors: Kupietzky Amram , Ariche Arie
Published: December 2022

Intrahepatic cholangiocarcinoma (ICC) is a rare and aggressive malignancy. It originates from the bile ducts and is the second most common primary cancer of the liver. Surgery is considered the only curative treatment of ICC, offering the best chance for long-term survival. The purpose of this article is to review the available literature on ICC, with a focus on the various aspects of the surgical care in this potentially lethal malignancy.
Catalytic Degradation of Toluene over MnO2/LaMnO3: Effect of Phase Type of MnO2 on Activity
Authors: Li Lu , Liu Yuwei , Liu Jingyin , Zhou Bing , Guo Mingming , Liu Lizhong
Published: December 2022

Series of α, β, γ, δ type MnO2 supported on LaMnO3 perovskite was developed by a one-pot synthesis route. Compared with α-MnO2, β-MnO2, γ-MnO2, δ-MnO2 and LaMnO3 oxides, all MnO2/LaMnO3 showed promotional catalytic performance for toluene degradation. Among them, α-MnO2/LaMnO3 holds the best active and mineralization efficiency. By the analysis of N2 adsorption-desorption, XPS and H2-TPR, it can be inferred that the improved activity should be ascribed to the higher proportion of lattice oxygen, better low-temperature reducibility and larger specific surface area. Besides, the byproducts from the low-temperature reaction of toluene oxidation were detected by a TD/GC-MS, confirming the presence of the intermediates. Combined with the in-situ DRIFTS, the catalytic degradation path of toluene oxidation has also been discussed in depth.
Electrochemical Detection of Sulfite by Electroreduction Using a Carbon Paste Electrode Binder with N-octylpyridinium Hexafluorophosphate Ionic Liquid
Authors: Villalobos Maicol Bustos , Ibarra José , Gidi Leyla , Cavieres Valentina , Aguirre María Jesús , Ramírez Galo , Arce Roxana
Published: December 2022

Sulfite is a widely used additive in food and beverages, and its maximum content is limited by food regulations. For this reason, determining the sulfite concentration using fast, low-cost techniques is a current challenge. This work describes the behavior of a sensor based on an electrode formed by carbon nanotubes an ionic liquid as binder, which by electrochemical reduction, allows detecting sulfite with a detection limit of 1.6 ± 0.05 mmol L−1 and presents adequate sensitivity. The advantage of detecting sulfite by reduction and not by oxidation is that the presence of antioxidants such as ascorbic acid does not affect the measurement. The electrode shown here is low-cost and easy to manufacture, robust, and stable.
CBP and p300 Jointly Maintain Neural Progenitor Viability but Play Unique Roles in the Differentiation of Neural Lineages
Authors: González-Martínez Rocío , Márquez-Galera Angel , Del Blanco Beatriz , López-Atalaya Jose P. , Barco Angel , Herrera Eloísa
Published: December 2022

The paralogous lysine acetyltransferases 3 (KAT3), CBP and P300, play critical roles during neurodevelopment, but their specific roles in neural precursors maintenance and differentiation remain obscure. In fact, it is still unclear whether these proteins are individually or jointly essential in processes such as proliferation of neural precursors, differentiation to specific neural cell types, or both. Here, we use subventricular zone-derived neurospheres as a potential ex vivo developmental model to analyze the proliferation and differentiation of neural stem cells (NSCs) lacking CBP, p300, or both proteins. The results showed that CBP and p300 are not individually essential for maintenance and proliferation of NSCs, although their combined ablation seriously compromised cell division. In turn, the absence of either of the two proteins compromised the differentiation of NSC into the neuronal and astrocytic lineages. Single-nucleus RNA sequencing analysis of neural cell cultures derived from CBP or p300 mutant neurospheres revealed divergent trajectories of neural differentiation upon CBP or p300 ablation, confirming unique functions and nonredundant roles in neural development. These findings contribute to a better understanding of the shared and individual roles of KAT3 proteins in neural differentiation and the etiology of neurodevelopmental disorders caused by their deficiency.
CFD-Simulation of Isobutane Dehydrogenation for a Fluidized Bed Reactor
Authors: Solovev Sergei A. , Soloveva Olga V. , Bekmukhamedov Giyjaz E. , Egorova Svetlana R. , Lamberov Alexander A.
Published: December 2022

In the present study, a mathematical model of the isobutane dehydrogenation process for a laboratory reactor with a diameter of 2.8 cm and a height of 70 cm was created using CFD methods. A two-fluid model was selected as a model for the fluidization simulation, when the gas and solid granular phases were considered as continuous. The model of chemical kinetics considers three reactions that make the main contribution to the products mass fraction at the reactor outlet: the reaction of catalytic dehydrogenation of isobutane to isobutylene, the reaction of thermal cracking of isobutylene with the formation of methane and propylene, and the reaction of catalytic hydrogenation of propylene. The model was verified in a series of experimental studies. Experimental studies and numerical simulations were carried out for the process parameters: gas velocity 0.008, 0.012 and 0.016 m/s, gas temperature 550, 575, 600 and 625 °C, and catalyst mass 75, 100 and 125 g. The optimal process temperature was 575 °C, where the yield of isobutylene averaged 47.6% of the mass. As the temperature decreased, the yield of isobutylene decreased to 40.1% by weight on average. With an increase in temperature, the yield of isobutylene increased to 52.8% by weight on average, and the total yield of products of side reactions increased to 20% by weight on average. Changes in the gas velocity and catalyst mass had an insignificant effect on the values of the yield of isobutylene, but significantly affected the values of the yield of the by-products.
Infodemiology of RSV in Italy (2017–2022): An Alternative Option for the Surveillance of Incident Cases in Pediatric Age?
Authors: Riccò Matteo , Baldassarre Antonio , Provenzano Sandro , Corrado Silvia , Cerviere Milena Pia , Parisi Salvatore , Marchesi Federico , Bottazzoli Marco
Published: December 2022

The aim of this study was to evaluate whether or not online queries for Respiratory Syncytial Virus (RSV) retrieved by means of Google Trends™ and the Italian Wikipedia analysis program mirror the occurrence of influenza-like illnesses (ILI), as reported by the Italian Influenza Surveillance network (InfluNet). Estimated rates for ILI in the general population and in the age groups 0–4 years and 5–14 years were obtained for the influenza seasons 2017–2018 to 2020–2021. Similarly, a weekly fraction of online searches was retrieved for a series of terms associated with Respiratory Syncytial Virus. Next, trends for daily visualization of Italian Wikipedia Pages for Human Respiratory Syncytial Virus, Pneumonia, Bronchiolitis, Influenza, and Respiratory Failure were similarly retrieved. The correlation of all search terms with ILI was analyzed by means of Spearman’s rank correlation analysis. Among search terms associated with the clinical diagnosis of Respiratory Syncytial Virus infections, the occurrence of ILI was highly correlated only with Bronchiolitis in the age group 0–4 years (β 0.210, p = 0.028), while more generic search terms, such as Bronchitis, fever, influenza, and Pneumonia, were identified as effective predictors of ILI, in general and by age groups. In a regression analysis modeled with ILIs as the outcome variable, daily visualizations for the Wikipedia pages on Bronchiolitis were identified as negative predictors for ILI in general (β = −0.152, p = 0.032), ILI in age group 0–4 years (β = −0.264, p = 0.001) and 5–14 years (β = −0.202, p = 0.006), while Influenza was characterized as a positive effector for ILIs in the age group 5–14 years (β = 0.245, p = 0.001). Interestingly, not only were the search terms extensively correlated with one another, but all of them were also characterized by autocorrelation through a Durbin-Watson test (all estimates DW < 2.0) In summary, our study identified a complicated pattern of data visualization as no clear association between rates of ILI in pediatric age group 0–4 and 5 to 14 years was actually found. Finally, our data stress that the infodemiology option may be quite problematic for assessing the time trend of RSV infections in Italy until more appropriate reporting will be made available, by sharing estimates of Lower Respiratory Tract Infections, and through a more accurate characterization of younger age groups.
Influence of Cleft Lip and Palate on Oral Health-Related Quality of Life in Northern Italy: Exploring Both the Children’s and Caregivers’ Perspectives
Authors: Defabianis Patrizia , Ninivaggi Rossella , Romano Federica
Published: December 2022

The aim of this cross-sectional study was to examine whether parents/caregivers’ perceptions of oral health-related quality of life (OHRQoL) differ from that of their cleft children, exploring the impact of demographic variables and cleft type on their agreement. Fifty-three primary and secondary schoolchildren, with non-syndromic orofacial cleft, and their parents answered the Child Oral Health Impact Profile (COHIP) questionnaire. Concordance between caregivers’ and children’s reports was low to moderate. Parents/caregivers had worse perceptions of OHRQoL compared to that of their children, peer interaction and functional well-being domains being statistically significantly different (p = 0.033 and p = 0.005, respectively). Cleft type, gender and parents’ country of origin seemed to be potential contributing factors of disagreement. Parents overestimated the impact of unilateral (p = 0.047) and bilateral cleft lip and palate (p = 0.021) on OHRQoL, and they rated more poorly than their male children did. Italian parents were more concerned about the functional well-being of their children (p = 0.014), and foreign parents about peer interaction (p = 0.010) and school environment (p = 0.012) dimensions. These findings suggest that parental assessment of OHRQoL cannot replace that of school-aged children, but they are complementary as they cover different, but equally relevant perspectives.