bluuebunny/crossref_metadata_2025 huggingface dataset:
Coaching Approach as a Sustainable Means of Improving the Skills of Management Students
Authors: Seemann Peter , Štofková Zuzana , Poliaková Adela , Biňasová Vladimíra , Loučanová Erika
Published: May 2024

The impetus for the development of coaching as a professional managerial activity is based on the needs and requirements of a modern sustainable society. This paper aims to explore the awareness of academic students about the coaching approach and to formulate recommendations for its application in the academic environment so that students are prepared for the requirements of practice. This article focuses on the views of experts in the field of the coaching approach as a means of improving communication in the academic environment, characterizes communication skills and the coaching approach, and describes coaching models and their benefits and barriers. This research aims to survey the awareness of the coaching approach among college and university students in Slovakia and the possibilities of its application in the academic environment. The primary research was carried out by the inquiry method with 689 students. An evaluation of the questionnaire and a statistical evaluation of the established hypotheses and assumptions concerning the coaching approach were conducted. According to the survey, 468 students, representing 68% of respondents, had knowledge about coaching. Furthermore, 68% of students had or have doubts about achieving their goals during their studies. According to the results of the survey, only 24% of students had experience with coaching. Cooperation with a coach was regarded as beneficial for 76% of the students who had already experienced coaching lectures who took part in the survey. The proposition of the students was the implementation of the subject “Foundations of coaching”, which could be offered to be taught to all students at the University of Zilina, Slovakia. The students of managerial fields would benefit by enhancing their leadership skills and providing support to their working teams, completing their final thesis, searching for professional job opportunities, and also coping with everyday life and stressful situations. Finally, we suggest the organization of introductory full-day workshops in specific areas of coaching for students of management, who are future managers. This will help to raise the awareness of the coaching approach at the University of Zilina in cooperation with the Counseling and Career Center and its activities.
Genetic Diversity of HMW-GS and the Correlation of Grain Quality Traits in Bread Wheat (Triticum aestivum L.) in Hubei Province, China
Authors: Wang Xiaofang , An Yue , Chen Junpeng , Wang Mengwei , Wang Chengyang , Hua Wei , Wang Qifei , Gao Song , Zhang Daorong , Ling Dong , Ren Xifeng , Zhu Jinghuan
Published: May 2024

High-molecular-weight glutenin subunits (HMW-GS) are an important component of total cereal proteins in wheat. It is closely related to the processing quality of flour. Here, we analyzed allelic variations at the Glu-1 locus in 163 wheat accessions from Hubei Province, China with SDS-PAGE. Among the 15 alleles detected, alleles 1, 7+8, and 2+12 were the major alleles, and 7, 6+8, and 2+10 were rare alleles. The breeding lines had higher genetic diversity than the commercial varieties. Alleles 7 and 6+8 significantly reduced the grain protein content and wet gluten content of wheat. The “1, 7+9, 5+10” and “1, 14+15, and 2+12” allelic combinations significantly increased the grain protein content, hardness index, test weight, and wet gluten content of wheat. Alleles 7+9, 14+15, and 5+10 were identified as alleles related to high wheat quality. The “1, 7, 5+10”, “1, 6+8, 5+10”, “null, 7+9, 2+12”, “1, 14+15, 2+12”, and “1, 7+9, 5+10” allelic combinations had greater effects on wheat grain quality traits. These results demonstrate the effects of HMW-GS on wheat grain quality traits and provide a reference for the genetic improvement of wheat quality.
QTL Mapping for Bacterial Wilt Resistance in Eggplant via Bulked Segregant Analysis Using Genotyping by Sequencing
Authors: Xiao Xi’ou , Lin Wenqiu , Nie Heng , Duan Zhe , Liu Ke
Published: May 2024

The bacterial wilt disease caused by Ralstonia solanacearum is a significant threat to eggplant production. Breeding and promoting resistant varieties is one of the most effective methods to manage bacterial wilt. Conducting QTL (quantitative trait locus) mapping of resistant genes can substantially enhance the breeding of plant resistance to bacterial wilt. In this study, a population of 2200 F2 individuals derived from resistant and susceptible materials was utilized to establish extreme resistance and susceptibility pools. Following resequencing analysis of the parents and extreme pools, the QTL were examined using the DEEP-BSA software and QTLseqr R package (version 0.7.5.2). The results revealed that the detection of 10 QTL sites on chromosomes 5, 8, 9, and 11 by the five algorithms of the DEEP-BSA software. Additionally, the candidate region of 62 Mb–72 Mb on chromosome 5 was identified in all five algorithms of the DEEP-BSA software, as well as by the QTLseqr R package. Subsequent gene annotation uncovered 276 genes in the candidate region of 62 Mb–72 Mb on chromosome 5. Additionally, RNA-seq results indicated that only 13 genes had altered expression levels following inoculation with R. solanacearum in the resistant materials. Based on the expression levels, SMEL4_05g015980.1 and SMEL4_05g016110.1 were identified as candidate genes. Notably, SNP annotation identified a non-synonymous mutation in the exonic region of SMEL4_05g015980.1 and a variant in the promoter region of SMEL4_05g016110.1. The research findings have practical significance for the isolation of bacterial wilt resistance genes in eggplant and the development of resistance to bacterial wilt varieties in eggplant.
Biochar Combined with Garbage Enzyme Enhances Nitrogen Conservation during Sewage Sludge Composting: Evidence from Microbial Community and Enzyme Activities Related to Ammoniation
Authors: Jiang Jishao , Cui Huilin , Bhople Parag , Chater Caspar C. C. , Yu Fuqiang , Liu Dong
Published: May 2024

Nitrogen loss is an unavoidable problem during composting processes, and the ammonia oxidation process significantly affects nitrogen transformation. The objective of this study was to evaluate nitrogen transformation when garbage enzyme (GE), biochar (BC), pelelith (PL) and combinations thereof were added during sewage sludge composting. Meanwhile, the succession of ammonia-oxidizing bacteria (AOB) and archaea (AOA) were also explored via quantitative polymerase chain reaction and high-throughput sequencing. The results showed that GE + BC and GE + PL treatments decreased ammonia (NH3) formation by 23.8% and 8.3%, and that of nitrous oxide (N2O) by 25.7% and 26.3% relative to the control, respectively. Simultaneously, the GE, GE + BC, and GE + PL treatments boosted the succession of AOA and AOB, and increased the activities of ammonia monooxygenase (AMO) and hydroxylamine oxidoreductase (HAO) activities and the gene copies of AOA and AOB. The AMO activities, NH4-N, NO3-N, and C/N, significantly affect AOA and AOB community structures. The network analysis predicted that the AMO and HAO were secreted mainly by the unclassified_Archaea and norank_Crenarchaeota, whereas it also showed that the GE + BC improved microbial associations with AOA, enzymatic activity, and environmental factors. Thus, the addition of garbage enzyme and biochar appears to be a promising mitigation strategy to reduce nitrogen losses during the composting process.
Development of Monoclonal Antibody against PirB and Establishment of a Colloidal Gold Immunochromatographic Assay for the Rapid Detection of AHPND-Causing Vibrio
Authors: Dong Xuan , Xie Jingmei , Wang Liying , Li Xuan , Lou Haoyu , Wang Guohao , Huang Jie
Published: May 2024

Acute hepatopancreatic necrosis disease (AHPND) poses a significant threat to shrimp aquaculture worldwide, necessitating the accurate and rapid detection of the pathogens. However, the increasing number of Vibrio species that cause the disease makes diagnosis and control more difficult. This study focuses on developing a monoclonal antibody against the Photorhabdus insect-related (Pir) toxin B (PirB), a pivotal virulence factor in AHPND-causing Vibrio, and establishing a colloidal gold immunochromatographic assay for the enhanced early diagnosis and monitoring of AHPND. Monoclonal antibodies targeting PirB were developed and utilized in the preparation of colloidal-gold-labeled antibodies for the immunochromatographic assay. The specificity and sensitivity of the assay were evaluated through various tests, including antibody subclass detection, affinity detection, and optimal labeling efficiency assessment. The developed PirB immunochromatographic test strips exhibited a good specificity, as demonstrated by the positive detection of AHPND-causing Vibrio and negative results for non-AHPND-causing Vibrio. The study highlights the potential of the developed monoclonal antibody and immunochromatographic assay for the effective detection of AHPND-causing Vibrio. Further optimization is needed to enhance the sensitivity of the test strips for improved practical applications in disease prevention and control in shrimp aquaculture.
A Case of Severe Abomasal Sand Impaction in a Farmed White-Tailed Deer (Odocoileus virginianus) in Florida
Authors: Rahmani Shahraki Alireza , Bittar João H. J. , Wisely Samantha M. , Campos-Krauer Juan M.
Published: May 2024

The University of Florida’s Cervidae Health Research Initiative (CHeRI) conducted a post-mortem examination of a two-year-old white-tailed doe deceased at a northern Florida white-tailed deer farm. The carcass of the deer had notable emaciation and bloating. Upon opening of the carcass, there was pneumonia and the rumen was tympanic and enlarged. Additionally, the abomasum was distended and contained approximately 5 kg of sand. It is not uncommon for white-tailed deer to engage in geophagia (eating soil or sand), which typically does not result in diseases or fatalities. However, in this animal, we suspect a chronic process that created a physical barrier, hindering nutrient absorption and resulting in physical irritation of the abomasal mucosa with subsequent inflammation. This may have caused a disturbance in immune system function, allowing opportunistic bacteria to colonize and invade other organs, such as the lungs, contributing to the animal’s death.
Effect of High Molybdenum Diet on Copper Status, Growth Performance, Blood Metabolites, Select Liver and Kidney Minerals, and Immune Responses of Boer Crosses
Authors: Solaiman Sandra G. , Beguesse Kyla A. , Min Byeng R.
Published: May 2024

This study examined the effects of elevated molybdenum (Mo) in goat diets on the growth, blood parameters, and immune responses in goats. Eighteen Boer crosses goats (BW = 25.6 ± 1.03 kg) were randomly assigned to three treatment groups: (1) control (no additional Mo), (2) 5 ppm Mo, and (3) 10 ppm Mo as ammonium molybdate was added to the grain mix. Animals were fed a 50:50 hay:grain diet ad libitum twice daily. Daily feed refusals were monitored, and intake was adjusted weekly. Body weights were recorded every 14 days and blood samples were collected on the second week of every month to determine Cu, Mo, Fe, Zn, and other blood metabolites. After 85 days, animals were humanely euthanized and carcass traits were measured. Liver, longissimus muscle area, and kidney samples were collected postmortem. Liver Cu (p < 0.003), blood triacylglycerides (p < 0.03), and serum total protein (p < 0.03) levels were reduced; the liver (p = 0.07) and kidney (p < 0.001) Mo concentrations were increased; and the immune response was decreased linearly (p < 0.01) with additional Mo. Low levels of Cu with increasing Mo levels in the diet did not negatively impact animal performance or blood metabolites, in the duration of this study (85 days); however, it lowered the liver Cu, Fe, and immune responses in goats.
Core Technology Topic Identification and Evolution Analysis Based on Patent Text Mining—A Case Study of Unmanned Ship
Authors: Lin Yan , Wang Xuelei , Yang Jing , Wang Shutian
Published: May 2024

Accurate identification and evolutionary analysis of core technology topics within patent texts play a crucial role in enabling enterprises to discern the development trajectory of core technologies, optimize research and development (R&D) strategies, and foster technological innovation. Based on the perspective of time series dynamic analysis, this study uses the Latent Dirichlet Allocation (LDA) topic modeling and TF-IDF text vectorization methods to comprehensively mine and identify patent technology topics in the field of unmanned ships. This study deeply analyzes the dynamic evolution of unmanned ship technology topics from two aspects: the evolution of technology theme intensity and the evolution of technology theme content. We refine the development characteristics and future development directions of unmanned ship technology. The findings reveal two hot technologies, six growth technologies, and six declining technologies in unmanned ship technology. Furthermore, the analysis of technical topic evolution illustrates a pattern of fragmentation, inheritance, and integration. This study advances the methodologies used for identifying and analyzing patent technology topics and helps to grasp the development rules and evolutionary trends of core technologies. In addition, this paper has reference value for the research and practice of core technology topic identification and evolution analysis methods based on patent text mining.
Hybrid Intelligence: Design for Sustainable Multiverse via Integrative Cognitive Creation Model through Human–Computer Collaboration
Authors: Liu Yuqi , Fu Zhiyong
Published: May 2024

The unprecedented development of artificial intelligence (AI) makes it possible for computers to imitate and surpass human intelligence (HI). Hybrid intelligence is the result of the co-evolution of AI and HI and has huge application potential in promoting the sustainable development of human society. This study starts from the similarities and differences between biological neural networks and artificial neural networks, compares the cognitive foundations of human intelligence and artificial intelligence, highlights the difference and connection between AI and HI, and puts forward the necessity and inevitability of their co-evolution to achieve hybrid intelligence with complementary advantages. Hybrid intelligence stands to become the pivotal force driving purposeful and planned sustainable creative behavior in the artificial intelligence era. This study proposes a design cognitive creation model based on human–computer collaboration that considers computational design thinking as the central concept. Moreover, the paradigm shift of design under hybrid intelligence intervention are explored from five aspects: “tool evolution”, “response mode”, “output result”, “iterative optimization” and “system innovation”. Finally, this article constructs a creative intervention mechanism of design creation driven by hybrid intelligence and discusses its role playing in the design activities of sustainable multiverse construction in the future. The proposal of the multiverse model transcends the confines of the metaverse’s virtual worldview and embraces sustainable development for value guidance. It advocates a future trajectory for humanity that hinges on technological progress, fostering a prosperous, balanced, and harmonious coexistence between the natureverse, socialverse, and digitalverse. This approach is not only rational and scientific, but also inherently sustainable.
Perturbed Dirac Operators and Boundary Value Problems
Authors: Liu Xiaopeng , Liu Yuanyuan
Published: May 2024

In this paper, the time-independent Klein-Gordon equation in R3 is treated with a decomposition of the operator Δ−γ2I by the Clifford algebra Cl(V3,3). Some properties of integral operators associated the kind of equations and some Riemann-Hilbert boundary value problems for perturbed Dirac operators are investigated.
Analyzing Richtmyer–Meshkov Phenomena Triggered by Forward-Triangular Light Gas Bubbles: A Numerical Perspective
Authors: Singh Satyvir , Msmali Ahmed Hussein
Published: May 2024

In this paper, we present a numerical investigation into elucidating the complex dynamics of Richtmyer–Meshkov (RM) phenomena initiated by the interaction of shock waves with forward-triangular light gas bubbles. The triangular bubble is filled with neon, helium, or hydrogen gas, and is surrounded by nitrogen gas. Three different shock Mach numbers are considered: Ms=1.12,1.21, and 1.41. For the numerical simulations, a two-dimensional system of compressible Euler equations for two-component gas flows is solved by utilizing the high-fidelity explicit modal discontinuous Galerkin technique. For validation, the numerical results are compared with the existing experimental results and are found to be in good agreement. The numerical model explores the impact of the Atwood number on the underlying mechanisms of the shock-induced forward-triangle bubble, encompassing aspects such as flow evolution, wave characteristics, jet formation, generation of vorticity, interface features, and integral diagnostics. Furthermore, the impacts of shock strengths and positive Atwood numbers on the flow evolution are also analyzed. Insights gained from this numerical perspective enhance our understanding of RM phenomena triggered by forward-triangular light gas bubbles, with implications for diverse applications in engineering, astrophysics, and fusion research.
Flexible Resource Allocation-Efficient Water Use Strategies Facilitate Invasion of Invasive Vine Sicyos angulatus L.
Authors: Pan Qingmin , Xue Chenyang , Meng Lin , Gao Ying , Yu Mengyang , Geng Lin , Guan Ping , Qu Bo
Published: May 2024

The invasive vine Sicyos angulatus L. destroys the natural ecosystem of invaded areas. Understanding the differences in growth and development between S. angulatus and other plants is necessary to explore the invasion mechanisms of S. angulatus and implement appropriate prevention and control measures. Thus, this study compared the growth, photosynthesis, and root characteristics of invasive liana S. angulatus and other three vine plants, Ipomoea nil (L.) Roth, Ipomoea purpurea (L.), and Thladiantha dubia Bunge, at different growth stages: seedling, flowering, and fruiting. The results showed that the total biomass of S. angulatus in the fruiting stage was 3–6 times that of the other three plants, and the root biomass ratio and root–shoot ratio decreased throughout the growth stage. Throughout the growth stage, the total leaf area of S. angulatus was significantly higher than that of the other three plant types, and the specific leaf area of S. angulatus at the seedling and flowering stages was 2.5–3 and 1.4–3 times that of the other three plants, respectively. The photosynthetic rate, stomatal conductance, and transpiration rate of S. angulatus at the fruiting stage were significantly higher than those of the other three plants, and its water use efficiency was higher than that of the other three plants at the three growth stages, indicating its strong photosynthetic capacity. The root activity and root pressure of S. angulatus were also significantly higher than those of the other three plants at the seedling and flowering stages. These results show that S. angulatus flexibly allocates resources to its aboveground parts during the growth stage to ensure that the plant obtains the space necessary for its growth and development and that with the help of higher root pressure and root activity, S. angulatus can maintain higher photosynthesis and water use efficiency with fewer resources. Therefore, the prevention and control of S. angulatus requires a combination of aboveground and underground measures. Spraying conventional weedicide/herbicide and manually removing aboveground plants may lead to its resurgence.
Swimming at Increasing Speeds in Steady and Unsteady Flows of Atlantic Salmon Salmo salar: Oxygen Consumption, Locomotory Behaviour and Overall Dynamic Body Acceleration
Authors: Agbeti Wisdom E. K. , Palstra Arjan P. , Black Suzy , Magnoni Leonardo , Lankheet Martin , Komen Hans
Published: May 2024

The swimming performance of cultured finfish species is typically studied under steady flow conditions. However, flow conditions are mostly unsteady, for instance, as experienced in sea pens in exposed sea areas. Using a Loligo swim tunnel, we investigated the effects of swimming in steady and unsteady flows at increasing swimming speeds on post-smolt Atlantic salmon. Oxygen consumption (MO2), locomotory behaviour, and overall dynamic body acceleration (ODBA), as determined with implanted acoustic sensor tags, were compared between both flow conditions. Results were obtained for mean swimming speeds of 0.2 to 0.8 m.s−1 under both flow conditions. Sensor tags that were implanted in the abdominal cavity had no significant effects on MO2 and locomotory parameters. The MO2 of fish swimming in unsteady flows was significantly higher (15–53%) than when swimming in steady flows (p < 0.05). Significant interaction effects of ODBA with flow conditions and swimming speed were found. ODBA was strongly and positively correlated with swimming speed and MO2 in unsteady flow (R2 = 0.94 and R2 = 0.93, respectively) and in steady flow (R2 = 0.91 and R2 = 0.82, respectively). ODBA predicts MO2 well over the investigated range of swimming speeds in both flow conditions. In an unsteady flow condition, ODBA increased twice as fast with MO2 compared with steady flow conditions (p < 0.05). From these results, we can conclude that (1) swimming in unsteady flow is energetically more costly for post-smolt Atlantic salmon than swimming in steady flow, as indicated by higher MO2, and (2) ODBA can be used to estimate the oxygen consumption of post-smolt Atlantic salmon in unsteady flow in swim tunnels.
In-Cell Testing of Zinc-Dependent Histone Deacetylase Inhibitors in the Presence of Class-Selective Fluorogenic Substrates: Potential and Limitations of the Method
Authors: Kleymenova Alla , Zemskaya Anastasia , Kochetkov Sergey , Kozlov Maxim
Published: May 2024

The development of anticancer drugs based on zinc-dependent histone deacetylase inhibitors (HDACi) has acquired great practical significance over the past decade. The most important HDACi characteristics are selectivity and strength of inhibition since they determine the mechanisms of therapeutic action. For in-cell testing of the selectivity of de novo-synthesized HDACi, Western blot analysis of the level of acetylation of bona fide protein substrates of HDACs of each class is usually used. However, the high labor intensity of this method prevents its widespread use in inhibitor screening. We developed an in-cell high-throughput screening method based on the use of three subtype-selective fluorogenic substrates of the general structure Boc-Lys(Acyl)-AMC, which in many cases makes it possible to determine the selectivity of HDACi at the class level. However, we found that the additional inhibitory activity of HDACi against metallo-β-lactamase domain-containing protein 2 (MBLAC2) leads to testing errors.
Effect of Frequency–Amplitude Parameter and Aspect Ratio on Propulsion Performance of Underwater Flapping-Foil
Authors: Ding Hao , Chen Ruoqian , Zhu Yawei , Shen Huipeng , Gao Qiang
Published: May 2024

The propulsion system is the core component of unmanned underwater vehicles. The flapping propulsion method of marine animals’ flippers, which allows for flexibility, low noise, and high energy utilization at low speeds, can provide a new perspective for the development of new propulsion technology. In this study, a new experimental flapping propulsion apparatus that can be installed in both directions has been constructed. The guide rail slider mechanism can achieve the retention of force in the direction of movement, thereby decoupling thrust, lift, and torque. Subsequently, the motion parameters of frequency–amplitude related to the thrust and lift of a bionic flapping-foil are scrutinized. A response surface connecting propulsion efficiency and these motion parameters is formulated. The highest efficiency of the flapping-foil propulsion is achieved at a frequency of 2 Hz and an amplitude of 40°. Furthermore, the impact of the installation mode and the aspect ratio of the flapping-foil is examined. The reverse installation of the swing yields a higher thrust than the forward swing. As the chord length remains constant and the span length increases, the propulsive efficiency gradually improves. When the chord length is extended to a certain degree, the propulsion efficiency exhibits a parabolic pattern, increasing initially and then diminishing. This investigation offers a novel perspective for the bionic design within the domain of underwater propulsion. This research provides valuable theoretical guidance for bionic design in the underwater propulsion field.
The Effect of Iron Oxide Insertion on the In Vitro Bioactivity, and Antibacterial Properties of the 45S5 Bioactive Glass
Authors: Hammami Imen , Jakka Suresh Kumar , Sá-Nogueira Isabel , Borges João Paulo , Graça Manuel Pedro Fernandes
Published: May 2024

The aging population and increasing incidence of trauma among younger age groups have heightened the increasing demand for reliable implant materials. Effective implant materials must demonstrate rapid osseointegration and strong antibacterial properties to ensure optimal patient outcomes and decrease the chance of implant rejection. This study aims to enhance the bone–implant interface by utilizing 45S5 bioglass modified with various concentrations of Fe3O4 as a coating material. The effect of the insertion of Fe3O4 into the bioglass structure was studied using Raman spectroscopy which shows that with the increase in Fe3O4 concentration, new vibration bands associated with Fe-related structural units appeared within the sample. The bioactivity of the prepared glasses was evaluated using immersion tests in simulated body fluid, revealing the formation of a calcium phosphate-rich layer within 24 h on the samples, indicating their potential for enhanced tissue integration. However, the sample modified with 8 mol% of Fe3O4 showed low reactivity, developing a calcium phosphate-rich layer within 96 h. All the bioglasses showed antibacterial activity against the Gram-positive and Gram-negative bacteria. The modified bioglass did not present significant antibacterial properties compared to the bioglass base.
The Absence of Attentional Bias to Low-Calorie Food Stimuli in Restrictive Dieters: Differences in the Allocation of Attentional Resources to High-Calorie Foods
Authors: Jiang Wu , Zhang Huan , Yang Haoping , Zhang Zonghao , Wang Aijun
Published: May 2024

Restrictive dieters are those who expect to achieve body shape and weight control through dieting. However, they often have difficulty suppressing the desire to consume food when confronted with it. It has been shown that when high- and low-calorie foods are presented together, the attention of restrictive eaters is preferentially directed to high-calorie foods. However, whether attentional bias occurs when low-calorie foods are present alone and whether the allocation of attentional resources is consistent with that for high-calorie foods has yet to be explored. The present study focused on the effects of high-/low-calorie foods on attentional bias in restrictive dieters. Seventy-eight participants were recruited to participate in the experiment via the Dutch Eating Behavior Questionnaire (DEBQ) scale, which is administered in a rapid serial visual presentation (RSVP) task. The results revealed that failed restrictive dieters had the lowest percentage of correct answers at the lag2 level, indicating attentional bias. Failed restrictive dieters allocated more attentional resources to high-calorie foods than to low-calorie foods. Restrictive dieters showed no attentional bias when low-calorie foods were presented alone. The results suggest that low-calorie foods do not elicit an attentional bias in restrictive dieters and that the allocation of attentional resources is not consistent when compared to that for high-calorie foods.
Proton Beam Therapy for Treating Patients with Hepatocellular Carcinoma with Major Portal Vein Tumor Invasion: A Single Center Retrospective Study
Authors: Ishida Toshiki , Mizumoto Masashi , Saito Takashi , Okumura Toshiyuki , Miura Kosei , Makishima Hirokazu , Iizumi Takashi , Numajiri Haruko , Baba Keiichiro , Murakami Motohiro , Nakamura Masatoshi , Nakai Kei , Sakurai Hideyuki
Published: May 2024

Hepatocellular carcinoma (HCC) with portal vein tumor thrombosis (PVTT) has a poor prognosis and is generally not indicated for surgery. Proton beam therapy (PBT) may offer an alternative treatment. In this study, long-term outcomes were examined in 116 patients (median age 66 years, 100 males) with HCC with advanced PVTT (Vp3 or Vp4) who received PBT from April 2008 to March 2018. Of these patients, 63 received PBT as definitive treatment and 53 as palliative treatment. The representative dose was 72.6 Gy (RBE) in 22 fractions. Eight patients died in follow-up, including 72 due to tumor progression. The 5-year overall survival (OS) rate was 18.0% (95% CI 9.8–26.2%) and the 5-year local control (LC) rate was 86.1% (74.9–97.3%). In multivariate analyses, performance status and treatment strategy were significantly associated with OS. The median follow-up period for survivors with definitive treatment was 33.5 (2–129) months, and the 5-year OS rate was 25.1% (12.9–37.3%) in these cases. The median survival time after definitive irradiation was >20 months. The 5-year OS rate was 9.1% (0–19.7%) for palliative irradiation. These results compare favorably with those of other therapies and suggest that PBT is a useful option for cases of HCC with advanced PVTT that cannot undergo surgery, with an expected survival benefit and good local control. Determining the optimal indication for this treatment is a future challenge.
Outcomes of Radical Hysterectomy for Early-Stage Cervical Carcinoma, with or without Prior Cervical Excision Procedure
Authors: Nasioudis Dimitrios , Labban Nayla , Gysler Stefan , Ko Emily M. , Giuntoli Robert L. , Kim Sarah H. , Latif Nawar A.
Published: May 2024

Objective: To investigate the impact of a prior cervical excisional procedure on the oncologic outcomes of patients with apparent early-stage cervical carcinoma undergoing radical hysterectomy. Methods: The National Cancer Database (2004–2015) was accessed, and patients with FIGO 2009 stage IB1 cervical cancer who had a radical hysterectomy with at least 10 lymph nodes (LNs) removed and a known surgical approach were identified. Patients who did and did not undergo a prior cervical excisional procedure (within 3 months of hysterectomy) were selected for further analysis. Overall survival (OS) was evaluated following the generation of Kaplan–Meier curves and compared with the log-rank test. A Cox model was constructed to control a priori-selected confounders. Results: A total of 3159 patients were identified; 37.1% (n = 1171) had a prior excisional procedure. These patients had lower rates of lymphovascular invasion (29.2% vs. 34.9%, p = 0.014), positive LNs (6.7% vs. 12.7%, p < 0.001), and a tumor size >2 cm (25.7% vs. 56%, p < 0.001). Following stratification by tumor size, the performance of an excisional procedure prior to radical hysterectomy was associated with better OS even after controlling for confounders (aHR: 0.45, 95% CI: 0.30, 0.66). The rate of minimally invasive surgery was higher among patients who had a prior excisional procedure (61.5% vs. 53.2%, p < 0.001). For these patients, performance of minimally invasive radical hysterectomy was not associated with worse OS (aHR: 1.37, 95% CI: 0.66, 2.82). Conclusions: For patients undergoing radical hysterectomy, preoperative cervical excision may be associated with a survival benefit. For patients who had a prior excisional procedure, minimally invasive radical hysterectomy was not associated with worse overall survival.
Distinct Capabilities in NAD Metabolism Mediate Resistance to NAMPT Inhibition in Glioblastoma
Authors: Perryman Richard , Chau Tsz Wing , De-Felice John , O’Neill Kevin , Syed Nelofer
Published: May 2024

Glioblastoma (GBM) cells require high levels of nicotinamide adenine dinucleotide (NAD) to fuel metabolic reactions, regulate their cell cycle and support DNA repair in response to chemotherapy and radiation. Inhibition of a key enzyme in NAD biosynthesis, NAMPT, has demonstrated significant anti-neoplastic activity. Here, we sought to characterise NAD biosynthetic pathways in GBM to determine resistance mechanisms to NAD inhibitors. GBM cells were treated with the NAMPT inhibitor FK866 with and without NAD precursors, and were analysed by qPCR, Western blot and proliferation assays (monolayer and spheroid). We also measured changes in the cell cycle, apoptosis, NAD/NADH levels and energy production. We performed orthoptic xenograft experiments in athymic nude mice to test the efficacy of FK866 in combination with temozolomide (TMZ). We show that the expression of key genes involved in NAD biosynthesis is highly variable across GBM tumours. FK866 inhibits proliferation, reduces NAD levels and limits oxidative metabolism, leading to G2/M cell cycle arrest; however, this can be reversed by supplementation with specific NAD precursors. Furthermore, FK866 potentiates the effects of radiation and TMZ in vitro and in vivo. NAMPT inhibitors should be considered for the treatment of GBM, with patients stratified based on their expression of key enzymes in other NAD biosynthetic pathways.
Childhood Cancer Incidence and Survival in South Australia and the Northern Territory, 1990–2017, with Emphasis on Indigenous Peoples
Authors: Mashtoub Suzanne , Ullah Shahid , Collinson Anne , Singh Gurmeet R. , Clark (Adnyamathanha) Justine , Leemaqz Shalem , Paltiel Ora , Roder David M. , Saxon Benjamin , McKinnon Ross , Pandol Stephen J. , Roberts Claire T. , Barreto Savio George
Published: May 2024

Background & Aims: Reports of a rise in childhood cancer incidence in Australia and globally prompted the investigation of cancer incidence and survival in South Australia (SA) and the Northern Territory (NT) over a 28-year period, with emphasis on Indigenous peoples. Methods: This cross-sectional analysis of two prospective longitudinal databases, the SA and NT Cancer Registries (1990–2017), included all reported cases of childhood cancers. Poisson regression provided estimates of incidence rate ratios and survival was modelled using Cox proportional hazard models for children aged <5 and ≥5 years. Results: A total of 895 patients across SA (N = 753) and the NT (N = 142) were ascertained. Overall and in the NT, childhood cancer incidence was higher in males compared with females (IRR 1.19 [1.04–1.35] and 1.43 [1.02–2.01], respectively). Lymphocytic leukemia was the most reported cancer type across all locations. With reference to the 1990–1999 era (181.67/100,000), cancer incidence remained unchanged across subsequent eras in the combined cohort (SA and NT) (2000–2009: 190.55/100,000; 1.06 [0.91–1.25]; 2010–2017: 210.00/100,000; 1.15 [0.98–1.35]); similar outcomes were reflected in SA and NT cohorts. Cancer incidence amongst non-Indigenous children significantly decreased from the 1990–1999 era (278.32/100,000) to the 2000–2009 era (162.92/100,000; 0.58 [0.35–0.97]). Amongst 39 Indigenous children in the NT, incidence rates remained unchanged across eras (p > 0.05). With reference to the 1990–1999 era, overall survival improved in subsequent eras in SA (2000–2009: HR 0.53 [0.38–0.73]; 2010–2017: 0.44 [0.28–0.68]); however, remained unchanged in the NT (2000–2009: 0.78 [0.40–1.51]; 2010–2017: 0.50 [0.24–1.05]). In the NT, overall survival of Indigenous patients was significantly lower compared with the non-Indigenous cohort (3.42 [1.92–6.10]). While the survival of Indigenous children with cancer significantly improved in the last two eras (p < 0.05), compared to the 1990–1999 era, no change was noted amongst non-Indigenous children in the NT (p > 0.05). Conclusions: The incidence of childhood cancers has remained unchanged over 28-years in SA and the NT. Encouragingly, improved survival rates over time were observed in SA and amongst Indigenous children of the NT. Nevertheless, survival rates in Indigenous children remain lower than non-Indigenous children.
Novel Catalytic Strategies for the Synthesis of Furans and Their Derivatives
Authors: Fulignati Sara
Published: May 2024

The depletion of fossil resources and their environmental issues make the transition toward alternative feedstock even more urgent [...]
New fac-[Re(CO)3(OO)(L)] and [Re(CO)2(OO)(L)2] Complexes Bearing Two Natural Food Additives, Maltol and Kojic Acid, as OO Ligands
Authors: Panagiotopoulou Angeliki , Kyprianidou Patricia , Tsoukalas Charalampos , Psycharis Vassilis , Raptopoulou Catherine P. , Pirmettis Ioannis , Papadopoulos Minas S. , Pelecanou Maria
Published: May 2024

The synthesis and structural characterization of new “2+1” mixed ligand fac-[Re(CO)3(OO)(L)] and Re(CO)2(OO)(L)2 complexes are reported herein. Maltol and kojic acid were chosen as bidentate OO ligands, while imidazole, isocyanocyclohexane or triphenylphosphine were selected as the monodentate ligands. The synthesis of the rhenium complexes was based on the reaction of [NEt4]2[Re(CO)3Br3] with maltol and kojic acid to generate the intermediate aqua complex fac-[Re(CO)3(OO)(H2O)], followed by the replacement of the labile aqua ligand by the monodentate ligand. Structural characterization of all Re complexes was established by NMR and IR spectroscopies, as well as two of them by single-crystal X-ray crystallography, revealing distorted octahedral geometry around the Re center. In the crystal lattice, the complexes form supramolecular networks due to the development of intermolecular interactions of the N-H⋯O, C-H⋯O and C-H⋯π type.
Prediction of Suitable Habitat of Alien Invasive Plant Ambrosia trifida in Northeast China under Various Climatic Scenarios
Authors: Chen Shengjie , Bai Xuejiao , Ye Ji , Chen Weiwei , Xu Guanghao
Published: May 2024

Ambrosia trifida is an invasive alien plant species, which has very high reproductive and environmental adaptability. Through strong resource acquisition ability and allelopathy, it could inhibit the growth and reproduction of surrounding plants and destroy the stability of an invasive ecosystem. It is very important to predict the change of suitable distribution area of A. trifida with climate change before implementing scientific control measures. Based on 106 A. trifida distribution data and 14 points of environmental data, the optimal parameter combination (RM = 0.1, FC = LQ) was obtained using the MaxEnt (version 3.4.1) model optimized by Kuenm package, and thus the potential suitable areas of A. trifida in Northeast China under three different climate scenarios (RCP2.6, RCP4.5, RCP8.5) with different emission intensities in the future (2050, 2070) were predicted. The changes of A. trifida suitable area in Northeast China under three climate scenarios were compared, and the relationship between the change of suitable area and emission intensity was analyzed. In general, the suitable area of A. trifida in Northeast China will expand gradually in the future, and the area of its highly suitable area will also increase with the increasing emission intensity, which is unfavorable to the control of A. trifida.
Aging of Superficial Musculoaponeurotic System of the Face—Novel Biomarkers and Micro-CT Relevance of Facial Anti-Gravity Support
Authors: Hînganu Marius Valeriu , Cucu Ramona Paula , Costan Victor-Vlad , Lozneanu Ludmila , Tamaș Camelia , Calistru Anca Elena , Hristian Liliana , Hînganu Delia
Published: May 2024

The soft superficial tissues of the face are against gravity through an intricate network of ligaments and ligamentous attachments. The aim of this investigation is to delineate the relationship between the muscular, fibrous, and vascular components of the superficial musculoaponeurotic system of the face (SMAS) at the level of its periosteal fixation areas from advanced radiological and novel biomarkers’ perspectives. These areas represent key points underlying skin aging and the longevity of restorative surgery results. Methods: This study was carried out on 37 surgical specimens, excised from patients admitted for surgery. On the excised specimens, we used special immunohistochemical techniques, such as markers for collagen type III, angiogenesis, vascular endothelium (I-CAM2) and muscle fibers (MYH2). We performed a micro-CT evaluation of these 37 specimens. Results: The results of this study showed different radiologic and IHC characteristics of the means of periosteal fixation of the SMAS. Evidence of morphohistological and radiological peculiarities of the retaining ligaments highlights new data for future functional studies of these structures. Our research must be continued with larger groups of subjects and through detailed methodological studies of vascular microperfusion and could represent an important new step in biotissue engineering and the customization of surgical techniques involving the sub-SMAS layers.
Evaluation of Spectrum-Aided Visual Enhancer (SAVE) in Esophageal Cancer Detection Using YOLO Frameworks
Authors: Chou Chu-Kuang , Karmakar Riya , Tsao Yu-Ming , Jie Lim Wei , Mukundan Arvind , Huang Chien-Wei , Chen Tsung-Hsien , Ko Chau-Yuan , Wang Hsiang-Chen
Published: May 2024

The early detection of esophageal cancer presents a substantial difficulty, which contributes to its status as a primary cause of cancer-related fatalities. This study used You Only Look Once (YOLO) frameworks, specifically YOLOv5 and YOLOv8, to predict and detect early-stage EC by using a dataset sourced from the Division of Gastroenterology and Hepatology, Ditmanson Medical Foundation, Chia-Yi Christian Hospital. The dataset comprised 2741 white-light images (WLI) and 2741 hyperspectral narrowband images (HSI-NBI). They were divided into 60% training, 20% validation, and 20% test sets to facilitate robust detection. The images were produced using a conversion method called the spectrum-aided vision enhancer (SAVE). This algorithm can transform a WLI into an NBI without requiring a spectrometer or spectral head. The main goal was to identify dysplasia and squamous cell carcinoma (SCC). The model’s performance was evaluated using five essential metrics: precision, recall, F1-score, mAP, and the confusion matrix. The experimental results demonstrated that the HSI model exhibited improved learning capabilities for SCC characteristics compared with the original RGB images. Within the YOLO framework, YOLOv5 outperformed YOLOv8, indicating that YOLOv5’s design possessed superior feature-learning skills. The YOLOv5 model, when used in conjunction with HSI-NBI, demonstrated the best performance. It achieved a precision rate of 85.1% (CI95: 83.2–87.0%, p < 0.01) in diagnosing SCC and an F1-score of 52.5% (CI95: 50.1–54.9%, p < 0.01) in detecting dysplasia. The results of these figures were much better than those of YOLOv8. YOLOv8 achieved a precision rate of 81.7% (CI95: 79.6–83.8%, p < 0.01) and an F1-score of 49.4% (CI95: 47.0–51.8%, p < 0.05). The YOLOv5 model with HSI demonstrated greater performance than other models in multiple scenarios. This difference was statistically significant, suggesting that the YOLOv5 model with HSI significantly improved detection capabilities.
A Lightweight and Dynamic Feature Aggregation Method for Cotton Field Weed Detection Based on Enhanced YOLOv8
Authors: Ren Doudou , Yang Wenzhong , Lu Zhifeng , Chen Danny , Su Wenxuan , Li Yihang
Published: May 2024

Weed detection is closely related to agricultural production, but often faces the problems of leaf shading and limited computational resources. Therefore, this study proposes an improved weed detection algorithm based on YOLOv8. Firstly, the Dilated Feature Integration Block is designed to improve the feature extraction in the backbone network by introducing large kernel convolution and multi-scale dilation convolution, which utilizes information from different scales and levels. Secondly, to solve the problem of a large number of parameters in the feature fusion process of the Path Aggregation Feature Pyramid Network, a new feature fusion architecture multi-scale feature interaction network is designed, which achieves the high-level semantic information to guide the low-level semantic information through the attention mechanism. Finally, we propose a Dynamic Feature Aggregation Head to solve the problem that the YOLOv8 detection head cannot dynamically focus on important features. Comprehensive experiments on two publicly accessible datasets show that the proposed model outperforms the benchmark model, with mAP50 and mAP75 improving by 4.7% and 5.0%, and 5.3% and 3.3%, respectively, whereas the number of model parameters is only 6.62 M. This study illustrates the utility potential of the algorithm for weed detection in cotton fields, marking a significant advancement of artificial intelligence in agriculture.
Applying Trust Patterns to Model Complex Trustworthiness in the Internet of Things
Authors: Messina Fabrizio , Rosaci Domenico , Sarnè Giuseppe M. L.
Published: May 2024

Key aspects of communities of the Internet of Things (IoT) smart objects presenting social aspects are represented by trust and reputation relationships between the objects. Several trustworthiness models have been presented in the literature in the context of multi-smart object community that could be adopted in the IoT scenario; however, most of these approaches represent the different dimensions of trust using scalar measures, then integrating these measures in a global trustworthiness value. In this paper, we discuss the limitation of this approach in the IoT context, highlighting the necessity of modeling complex trust relationships that cannot be captured by a vector-based model, and we propose a new trust model in which the trust perceived by an object with respect to another object is modeled by a directed, weighted graph whose vertices are trust dimensions and whose arcs represent relationships between trust dimensions. By using this new model, we provide the IoT community with the possibility of representing also situations in which an object does not know a trust dimension, e.g., reliability, but it is able to derive it from another one, e.g., honesty. The introduced model can represent any trust structure of the type illustrated above, in which several trust dimensions are mutually dependent.
Investigation of Hydrogen Flux Influence on InGaP Layer and Device Uniformity
Authors: Yang Shangyu , Guo Ning , Zhao Siqi , Li Yunkai , Wei Moyu , Zhang Yang , Liu Xingfang
Published: May 2024

In this study, we conduct a comprehensive examination of the influence of hydrogen (H2) carrier gas flux on the uniformity of epitaxial layers, specifically focusing on the InGaP single layer and the full structure of the InGaP/GaAs heterojunction bipolar transistor (HBT). The results show that an elevated flux of H2 carrier gas markedly facilitates the stabilization of layer uniformity. Optimal uniformity in epitaxial wafers is achievable at a suitable carrier gas flux. Furthermore, this study reveals a significant correlation between the uniformity of the InGaP single layer and the overall uniformity of HBT structures, indicating a consequential interdependence.