bluuebunny/crossref_metadata_2025 huggingface dataset:
The Biosynthesis of Liquid Fuels and Other Value-Added Products Based on Waste Glycerol—A Comprehensive Review and Bibliometric Analysis
Authors: Kazimierowicz Joanna , Dębowski Marcin , Zieliński Marcin , Ignaciuk Aneta , Mlonek Sandra , Cruz Sanchez Jordi
Published: June 2024

Waste glycerol can be subjected to various processing operations, including purification and refining, to obtain glycerol of an appropriate purity. Alternative methods for utilising waste glycerol are also being sought, e.g., by converting it into other valuable chemical products or biofuels. Therefore, various technologies are being developed to ensure effective and sustainable utilisation of this type of waste. The production of value-added products from waste glycerol strongly determines the improvement of the economic viability of biofuel production and corresponds to the model of a waste-free and emission-free circular economy. This paper characterises the mechanisms and evaluates the efficiency of existing methods for microbiological utilisation of waste glycerol into liquid biofuels, including biodiesel, bioethanol and biobutanol, and identifies further production avenues of value-added products. In addition, it presents the results of a bibliographical analysis of publications related to the production of liquid fuels and economically valuable products from glycerol, assesses the progress of research and application work and, finally, identifies areas for future research.
Exploring Residential Energy Behaviour of the Younger Generation for Sustainable Living: A Systematic Review
Authors: Peng Yehui , Gaspari Jacopo , Marchi Lia
Published: June 2024

The global energy crisis has spurred increased investments in energy efficiency and clean energy initiatives; however, the results have fallen short of expected effectiveness. Concurrently, population growth and urbanisation drive a persistent surge in energy demands, especially within the residential sector, significant to overall building energy consumption. Current research focuses on residents’ responses to one-shot investments for energy efficiency or clean sources. The renovation wave, involving a massive number of existing buildings, calls for the mobilisation of huge investments that can be hard to afford in the short run. Sustainable behavioural change is complementarily rising as a key asset for maximising the overall estimated energy saving potential. Despite significant efforts to analyse household energy use and promote behavioural transformations, the literature remains gaping about future users, particularly the younger generation, as future leaders of sustainable development who exhibit a more responsible approach towards climate-related issues but also a strong dependency on digital-based solutions, which may influence energy use patterns and living habits, also impacting relations among peers and overall societal sustainability and energy efficiency. This article proposes a systematic literature review to analyse the variables affecting young people’s energy behaviour at home. The aim is to investigate the engines and gaps between strategies or tools for behaviour change and the expected effects, then find potential methods to address that barrier to identify a more promising approach, encouraging the younger generation to translate towards more sustainable energy behaviours.
An Experimental Performance Assessment of a Passively Controlled Wind Turbine Blade Concept: Part A—Isotropic Materials
Authors: Papadakis Nikolaos , Condaxakis Constantinos
Published: June 2024

This paper is the first part of a two-part series, which presents preliminary findings on a novel flexible curved wind turbine blade designed for passive control, comparing its aerodynamic performance and behavior against a conventional straight blade. Characterized by its ability to twist around its longitudinal axis under bending loads, the flexible curved blade is engineered to self-regulate in response to varying wind speeds, optimizing power output and enhancing operational safety. This design utilizes inherent elasticity and specific geometric configurations to develop torsional loads, resulting in continuous adjustment of the blade’s pitch angle via twist–bend deformation. The study focuses on a comparative analysis conducted in a wind tunnel, testing both a small-scale model of the conventional blade and the flexible curved blade of equivalent diameter. Results indicate that the flexible curved blade concept successfully moderates its rotational speed and power output at higher wind speeds and demonstrates the capability to start generating power at lower wind speeds and stabilize power effectively, aligning with sustainability goals by potentially reducing reliance on active control systems. Despite promising outcomes, passive control mechanisms did not activate at the designed wind speeds, revealing a misalignment between expected and actual performance and underscoring the need for further refinements in blade design and control settings. Additionally, the power coefficient (Cp) versus tip speed ratio (TSR) comparison showed that flexible curved blades operate within a lower TSR range and exhibit controlled capping of power under high wind conditions, marked by a distinctive ‘hook-like’ feature in Cp behavior. This study confirms the feasibility of designing and manufacturing passively controlled wind turbine blades tailored to specific performance criteria and underscores the potential of such technology. Future work, to be detailed in a subsequent paper, will explore further optimizations and the use of Glass Fiber-Reinforced Polymer (GFPR) composite materials to enhance blade flexibility and performance.
Improving the Fuel Combustion Quality Control System in Medium Power Boilers
Authors: Janta-Lipińska Sylwia , Shkarovskiy Alexander , Chrobak Łukasz
Published: June 2024

The constant development of electronic devices and components allows for older systems (which have been well received) with electronic control of combustion processes in municipal thermal energy to be improved to new levels, with significant economic and ecological effects. This article presents details of the development of an improved natural gas combustion quality control system in the modernized DKVR 6.5-13 steam boiler. This paper presents the results of comparative tests of this boiler obtained for various variants of the system operation. It has been proven that maximum boiler efficiency indicators and minimum toxicity of exhaust gases discharged into the atmosphere can be achieved by using a proposed control system. The system uses the regulated residual chemical underburning method, based on simultaneous control of the oxygen and carbon dioxide content in the exhaust gases. As a result of the use of the proposed method, an increase in boiler efficiency was achieved by 1.5–2.1% compared with standard automatic regulation and an increase by 0.3–0.8% compared with regulation based only on the oxygen content in the exhaust gases. These results were achieved by reducing the excess air coefficient. In addition, nitrogen oxide emissions were reduced by 25–30%, which, with a justified safe carbon monoxide content in exhaust gases of up to 250 ppm, reduced the exhaust gas toxicity index to 20%.
Discrimination Experiences among Asian American and Pacific Islander Adults during the COVID-19 Pandemic and Their Association with Mental Health Outcomes: Updated Findings from the COMPASS Study
Authors: Dougan Marcelle M. , Tzuang Marian , Nam Bora , Meyer Oanh L. , Tsoh Janice Y. , Park Van M. Ta
Published: June 2024

Background: Reports of escalated discrimination experiences among Asian American and Native Hawaiian Pacific Islanders (AANHPI) continue. Methods: Using the original and follow-up surveys of the COVID-19 Effects on the Mental and Physical Health of AAPI (Asian American and Pacific Islanders) Survey Study (COMPASS I and COMPASS II) (n = 3177), we examined changes over approximately a 1-year period in discrimination experiences attributable to being AAPI and factors associated with worse mental health outcomes. Results: Experiences of discrimination remained high in COMPASS II with 60.6% (of participants (compared to 60.2% among the same people in COMPASS I) reporting one or more discrimination experiences, and 28.6% reporting worse mental health outcomes. Experiences of discrimination were associated with modest but significant increase in the odds of worse mental health: adjusted OR 1.02 (95% CI 1.01–1.04). Being younger, being of Native Hawaiian/Pacific Islander or Hmong descent (relative to Asian Indian), and having spent 50% or less of their lifetime in the US (vs. US born), were significantly associated with worse mental health. Conclusions: The fall-out from the pandemic continues to adversely impact AANHPI communities. These findings may help influence policy initiatives to mitigate its effects and support interventions designed to improve mental health outcomes.
Two-Year Results of a Five-Year Personalized Integrative Obesity Coaching Program (IBO) Based upon a Systems Health Perspective and an Evolutionary Longitudinal Study Approach
Authors: Brink Sander M. , Wortelboer Heleen M. , Hoff Ard F. ten , Emmelot Cornelis H. , Visscher Tommy L. S. , van Wietmarschen Herman A.
Published: June 2024

This study presents the outcomes of a 5-year personalized integrative coaching program for adults with obesity (body mass index BMI ≥ 30 kg/m2), based upon a systems health perspective, during the first 2 years. This longitudinal study, which had an evolutionary design, included all adults who enrolled in the program. Health-related quality of life (HRQoL) was measured with the Short Form-36 (SF-36), and physical outcomes included weight, waist circumference, aerobic capacity, lipid profile, and HbA1c. Subsequently, participants completed questionnaires (e.g., the Symptom Checlist-90 (SCL-90) and the Checklist Individual Strength (CIS)). Seventy-nine adults with a mean BMI of 39.5 kg/m2 (SD 5.3) were included. Forty-four participants completed 2 years in the program. Compared to baseline, there were significant improvements in the SF-36 subscales ‘physical functioning’ (MD 9.9 points, 95% CI: 2.1–17.5, p = 0.013) and ‘general health perceptions’ (MD 9.3 points, 95% CI 2.9–15.7, p = 0.006). Furthermore, significant improvements in physical outcomes and psychosocial questionnaires (e.g., weight loss (MD 3.5 kg, 95% CI: 1.2–5.7, p = 0.003), waist circumference (MD 5.1 cm, 95% CI: 2.4–7.8, p < 0.001), and CIS fatigue (MD 6.8, 95% CI: 3.1–10.5, p = 0.001) were observed. This study highlights the importance of a systems health perspective supporting the development of a personalized integrative coaching program for adults with obesity in a ‘real-world’ setting.
Genome-Wide Identification and Characterization of Lignin Synthesis Genes in Maize
Authors: Wang Shuai , Wang Xiaofang , Yue Liangxu , Li Huangai , Zhu Lei , Dong Zhenying , Long Yan
Published: June 2024

Lignin is a crucial substance in the formation of the secondary cell wall in plants. It is widely distributed in various plant tissues and plays a significant role in various biological processes. However, the number of copies, characteristics, and expression patterns of genes involved in lignin biosynthesis in maize are not fully understood. In this study, bioinformatic analysis and gene expression analysis were used to discover the lignin synthetic genes, and two representative maize inbred lines were used for stem strength phenotypic analysis and gene identification. Finally, 10 gene families harboring 117 related genes involved in the lignin synthesis pathway were retrieved in the maize genome. These genes have a high number of copies and are typically clustered on chromosomes. By examining the lignin content of stems and the expression patterns of stem-specific genes in two representative maize inbred lines, we identified three potential stem lodging resistance genes and their interactions with transcription factors. This study provides a foundation for further research on the regulation of lignin biosynthesis and maize lodging resistance genes.
Residence of the Nucleotide Sugar Transporter Family Members SLC35F1 and SLC35F6 in the Endosomal/Lysosomal Pathway
Authors: Van den Bossche François , Tevel Virginie , Gilis Florentine , Gaussin Jean-François , Boonen Marielle , Jadot Michel
Published: June 2024

The SLC35 (Solute Carrier 35) family members acting as nucleotide sugar transporters are typically localized in the endoplasmic reticulum or Golgi apparatus. It is, therefore, intriguing that some reports document the presence of orphan transporters SLC35F1 and SLC35F6 within the endosomal and lysosomal system. Here, we compared the subcellular distribution of these proteins and found that they are concentrated in separate compartments; i.e., recycling endosomes for SLC35F1 and lysosomes for SLC35F6. Swapping the C-terminal tail of these proteins resulted in a switch of localization, with SLC35F1 being trafficked to lysosomes while SLC35F6 remained in endosomes. This suggested the presence of specific sorting signals in these C-terminal regions. Using site-directed mutagenesis, fluorescence microscopy, and cell surface biotinylation assays, we found that the EQERLL360 signal located in the cytoplasmic tail of human SLC35F6 is involved in its lysosomal sorting (as previously shown for this conserved sequence in mouse SLC35F6), and that SLC35F1 localization in the recycling pathway depends on two YXXΦ-type signals: a Y367KQF sequence facilitates its internalization from the plasma membrane, while a Y392TSL motif prevents its transport to lysosomes, likely by promoting SLC35F1 recycling to the cell surface. Taken together, these results support that some SLC35 members may function at different levels of the endosomal and lysosomal system.
Cost-Efficient Expression of Human Cardiac Myosin Heavy Chain in C2C12 Cells with a Non-Viral Transfection Reagent
Authors: Berg Albin E. , Velayuthan Lok Priya , Månsson Alf , Ušaj Marko
Published: June 2024

Production of functional myosin heavy chain (MHC) of striated muscle myosin II for studies of isolated proteins requires mature muscle (e.g., C2C12) cells for expression. This is important both for fundamental studies of molecular mechanisms and for investigations of deleterious diseases like cardiomyopathies due to mutations in the MHC gene (MYH7). Generally, an adenovirus vector is used for transfection, but recently we demonstrated transfection by a non-viral polymer reagent, JetPrime. Due to the rather high costs of JetPrime and for the sustainability of the virus-free expression method, access to more than one transfection reagent is important. Here, we therefore evaluate such a candidate substance, GenJet. Using the human cardiac β-myosin heavy chain (β-MHC) as a model system, we found effective transfection of C2C12 cells showing a transfection efficiency nearly as good as with the JetPrime reagent. This was achieved following a protocol developed for JetPrime because a manufacturer-recommended application protocol for GenJet to transfect cells in suspension did not perform well. We demonstrate, using in vitro motility assays and single-molecule ATP turnover assays, that the protein expressed and purified from cells transfected with the GenJet reagent is functional. The purification yields reached were slightly lower than in JetPrime-based purifications, but they were achieved at a significantly lower cost. Our results demonstrate the sustainability of the virus-free method by showing that more than one polymer-based transfection reagent can generate useful amounts of active MHC. Particularly, we suggest that GenJet, due to its current ~4-fold lower cost, is useful for applications requiring larger amounts of a given MHC variant.
Chronic Treatment with Oxcarbazepine Attenuates Its Anticonvulsant Effect in the Maximal Electroshock Model in Mice
Authors: Borowicz-Reutt Kinga , Banach Monika
Published: June 2024

The objective of this study was to assess the impact of acute and chronic treatment with oxcarbazepine on its anticonvulsant activity, neurological adverse effects, and protective index in mice. Oxcarbazepine was administered in four protocols: once or twice daily for one week (7 × 1 or 7 × 2) and once or twice daily for two weeks (14 × 1 or 14 × 2). A single dose of the drug was employed as a control. The anticonvulsant effect was evaluated in the maximal electroshock test in mice. Motor and long-term memory impairment were assessed using the chimney test and the passive avoidance task, respectively. The concentrations of oxcarbazepine in the brain and plasma were determined via high-performance liquid chromatography. Two weeks of oxcarbazepine treatment resulted in a significant reduction in the anticonvulsant (in the 14 × 1; 14 × 2 protocols) and neurotoxic (in the 14 × 2 schedule) effects of this drug. In contrast, the protective index for oxcarbazepine in the 14 × 2 protocol was found to be lower than that calculated for the control. No significant deficits in memory or motor coordination were observed following repeated administration of oxcarbazepine. The plasma and brain concentrations of this anticonvulsant were found to be significantly higher in the one-week protocols. Chronic treatment with oxcarbazepine may result in the development of tolerance to its anticonvulsant and neurotoxic effects, which appears to be dependent on pharmacodynamic mechanisms.
Assessment of Molecular Markers in Pediatric Ovarian Tumors: Romanian Single-Center Experience
Authors: Bîcă Ovidiu , Ciongradi Carmen Iulia , Benchia Diana , Sârbu Ioan , Alecsa Mirabela , Cristofor Alexandra Elena , Bîcă Delia Elena , Lozneanu Ludmila
Published: June 2024

Pediatric ovarian tumors exhibit unique diagnostic and therapeutic challenges. This study evaluates the expression of SALL4 and OCT3/4 biomarkers in pediatric ovarian tumors and their associations with tumor subtype, stage, and clinical outcome. A retrospective analysis was conducted on 64 patients under 18 years old, examining demographic data, tumor characteristics, immunohistochemical staining, and clinical outcomes. Our results show that SALL4 was significantly expressed in adenocarcinoma, dysgerminoma (DSG), mixed germ cell tumors (GCTs), and immature teratoma, while OCT3/4 was highly expressed in DSG and mixed GCTs. Both markers are associated with a higher tumor grade and stage, indicating a more aggressive disease. The SALL4 positivity expression was correlated with high alpha fetoprotein (AFP) and lactate dehydrogenase (LDH) levels, while OCT3/4 positivity significantly predicted the risk of subsequent metastasis. The mean progression-free survival (PFS) was notably shorter in patients with positive markers. These findings underscore the diagnostic and prognostic value of SALL4 and OCT3/4 in pediatric ovarian tumors, aligning with previous research and supporting their use in clinical practice for better disease management and patient outcomes.
Latest Insights into the In Vivo Studies in Murine Regarding the Role of TRP Channels in Wound Healing—A Review
Authors: Grigore Alexandra , Coman Oana Andreia , Păunescu Horia , Costescu Mihnea , Fulga Ion
Published: June 2024

Wound healing involves physical, chemical and immunological processes. Transient receptor potential (TRP) and other ion channels are implicated in epidermal re-epithelization. Ion movement across ion channels can induce transmembrane potential that leads to transepithelial potential (TEP) changes. TEP is present in epidermis surrounding the lesion decreases and induces an endogenous direct current generating an epithelial electric field (EF) that could be implicated in wound re-epithelialization. TRP channels are involved in the activation of immune cells during mainly the inflammatory phase of wound healing. The aim of the study was to review the mechanisms of ion channel involvement in wound healing in in vivo experiments in murine (mice, rats) and how can this process be influenced. This review used the latest results published in scientific journals over the last year and this year to date (1 January 2023–31 December 3000) in order to include the in-press articles. Some types of TRP channels, such as TRPV1, TRPV3 and TRPA1, are expressed in immune cells and can be activated by inflammatory mediators. The most beneficial effects in wound healing are produced using agonists of TRPV1, TRPV4 and TRPA1 channels or by inhibiting with antagonists, antisense oligonucleotides or knocking down TRPV3 and TRPM8 channels.
Innovative Bi5O7I/MIL-101(Cr) Compounds: A Leap Forward in Photocatalytic Tetracycline Removal
Authors: Hong Jie , Chu Zhaohan , Li Claudia , Yang Wanliang , Kawi Sibudjing , Ye Qinong
Published: June 2024

In environmental chemistry, photocatalysts for eliminating organic contaminants in water have gained significant interest. Our study introduces a unique heterostructure combining MIL-101(Cr) and bismuth oxyiodide (Bi5O7I). We evaluated this nanostructure’s efficiency in adsorbing and degrading tetracycline (TC) under visible light. The Bi5O7I@MIL-101(Cr) composite, with a surface area of 637 m2/g, prevents self-aggregation seen in its components, enhancing visible light absorption. Its photocatalytic efficiency surpassed Bi5O7I and MIL-101(Cr) by 33.4 and 9.2 times, respectively. Comprehensive analyses, including scanning electron microscopy (SEM) and transmission electron microscopy (TEM), confirmed the successful formation of the heterostructure with defined morphological characteristics. BET analysis demonstrated its high surface area, while X-ray diffraction (XRD) confirmed its crystallinity. Electron spin resonance (ESR) tests showed significant generation of reactive oxygen species (ROS) like h+ and·•O2− under light, crucial for TC degradation. The material maintained exceptional durability over five cycles. Density functional theory (DFT) simulations and empirical investigations revealed a type I heterojunction between Bi5O7I and MIL-101(Cr), facilitating efficient electron–hole pair separation. This study underscores the superior photocatalytic activity and stability of Bi5O7I@MIL-101(Cr), offering insights into designing innovative photocatalysts for water purification.
Subtle Structural Differences Affect the Inhibitory Potency of RGD-Containing Cyclic Peptide Inhibitors Targeting SPSB Proteins
Authors: Li Kefa , Luo Yanhong , Hu Weiwei , Yang Jinjin , Zhang Danting , Wei Huan , You Tingting , Lin Hai-Shu , Kuang Zhihe
Published: June 2024

The SPRY domain-containing SOCS box proteins SPSB1, SPSB2, and SPSB4 utilize their SPRY/B30.2 domain to interact with a short region in the N-terminus of inducible nitric oxide synthase (iNOS), and recruit an E3 ubiquitin ligase complex to polyubiquitinate iNOS, resulting in the proteasomal degradation of iNOS. Inhibitors that can disrupt the endogenous SPSB-iNOS interactions could be used to augment cellular NO production, and may have antimicrobial and anticancer activities. We previously reported the rational design of a cyclic peptide inhibitor, cR8, cyclo(RGDINNNV), which bound to SPSB2 with moderate affinity. We, therefore, sought to develop SPSB inhibitors with higher affinity. Here, we show that cyclic peptides cR7, cyclo(RGDINNN), and cR9, cyclo(RGDINNNVE), have ~6.5-fold and ~2-fold, respectively, higher SPSB2-bindng affinities than cR8. We determined high-resolution crystal structures of the SPSB2-cR7 and SPSB2-cR9 complexes, which enabled a good understanding of the structure–activity relationships for these cyclic peptide inhibitors. Moreover, we show that these cyclic peptides displace full-length iNOS from SPSB2, SPSB1, and SPSB4, and that their inhibitory potencies correlate well with their SPSB2-binding affinities. The strongest inhibition was observed for cR7 against all three iNOS-binding SPSB proteins.
The miR-182-5p/GPX4 Pathway Contributes to Sevoflurane-Induced Ototoxicity via Ferroptosis
Authors: Jin Lin , Yu Xiaopei , Zhou Xuehua , Li Gang , Li Wen , He Yingzi , Li Huawei , Shen Xia
Published: June 2024

Our study aimed to investigate the role of ferroptosis in sevoflurane-induced hearing impairment and explore the mechanism of the microRNA-182-5p (miR-182-5p)/Glutathione Peroxidase 4 (GPX4) pathway in sevoflurane-induced ototoxicity. Immunofluorescence staining was performed using myosin 7a and CtBP2. Cell viability was assessed using the CCK-8 kit. Fe2+ concentration was measured using FerroOrange and Mi-to-FerroGreen fluorescent probes. The lipid peroxide level was assessed using BODIPY 581/591 C11 and MitoSOX fluorescent probes. The auditory brainstem response (ABR) test was conducted to evaluate the hearing status. Bioinformatics tools and dual luciferase gene reporter analysis were used to confirm the direct targeting of miR-182-5p on GPX4 mRNA. GPX4 and miR-182-5p expression in cells was assessed by qRT-PCR and Western blot. Ferrostatin-1 (Fer-1) pretreatment significantly improved hearing impairment and damage to ribbon synapses in mice caused by sevoflurane exposure. Immunofluorescence staining revealed that Fer-1 pretreatment reduced intracellular and mitochondrial iron overload, as well as lipid peroxide accumulation. Our findings indicated that miR-182-5p was upregulated in sevoflurane-exposed HEI-OC1 cells, and miR-182-5p regulated GPX4 expression by binding to the 3′UTR of GPX4 mRNA. The inhibition of miR-182-5p attenuated sevoflurane-induced iron overload and lipid peroxide accumulation. Our study elucidated that the miR-182-5p/GPX4 pathway was implicated in sevoflurane-induced ototoxicity by promoting ferroptosis.
The Development of an Isotope Dilution Mass Spectrometry Method for Interleukin-6 Quantification
Authors: Yu Zetao , Wang Jing , Xia Wenqiang , Wang Yuemin , Zhang Yafen , Tang Jintian , Cui Haifeng , Yang Xiaoying , Bao Chenchen , Ye Zihong
Published: June 2024

Inflammatory responses and tumor developments are closely related, with interleukin-6 (IL-6) playing important roles in both processes. IL-6 has been extensively identified as a potential tumor biomarker. This study developed an isotope dilution mass spectrometry (IDMS) method for quantifying IL-6 based on signature peptides. These peptides were screened by excluding those with missed cleavage or post-translational modification. The method’s accuracy was verified using amino acid-based IDMS, in which purified IL-6 protein samples were quantified after hydrolyzing them into amino acids, and no significant difference was observed (p-value < 0.05). The method demonstrated good linearity and sensitivity upon testing. The specificity and matrix effect of the method were verified, and a precision study showed that the coefficient of variation was less than 5% for both the intra-day and inter-day tests. Compared to immunoassays, this method offers distinct advantages, such as the facilitation of multi-target analysis. Furthermore, the peptides used in this study are much more convenient for storage and operation than the antibodies or purified proteins typically used in immunoassays.
Effect of the Height of a 3D-Printed Model on the Force Transmission and Thickness of Thermoformed Orthodontic Aligners
Authors: Ghoraba Omar , Bourauel Christoph , Aldesoki Mostafa , Singer Lamia , Ismail Ahmed M. , Elattar Hanaa , Alhotan Abdulaziz , Elshazly Tarek M.
Published: June 2024

This research aims to investigate the influence of model height employed in the deep drawing of orthodontic aligner sheets on force transmission and aligner thickness. Forty aligner sheets (Zendura FLX) were thermoformed over four models of varying heights (15, 20, 25, and 30 mm). Normal contact force generated on the facial surface of the upper right central incisor (Tooth 11) was measured using pressure-sensitive films. Aligner thickness around Tooth 11 was measured at five points. A digital caliper and a micro-computed tomography (µ-CT) were employed for thickness measurements. The normal contact force exhibited an uneven distribution across the facial surface of Tooth 11. Model 15 displayed the highest force (88.9 ± 23.2 N), while Model 30 exhibited the lowest (45.7 ± 15.8 N). The force distribution was more favorable for bodily movement with Model 15. Thickness measurements revealed substantial thinning of the aligner after thermoforming. This thinning was most pronounced at the incisal edge (50% of the original thickness) and least at the gingivo-facial part (85%). Additionally, there was a progressive reduction in aligner thickness with increasing model height, which was most significant on the facial tooth surfaces. We conclude that the thermoplastic aligner sheets undergo substantial thinning during the thermoforming process, which becomes more pronounced as the height of the model increases. As a result, there is a decrease in both overall and localized force transmission, which could lead to increased tipping by the aligner and a diminished ability to achieve bodily movement.
Experimental Investigation of Damping Properties of Selected Polymer Materials
Authors: Witek Lucjan , Łabuński Piotr
Published: June 2024

This paper presents the results of an experimental modal analysis of a beam covered by polymer materials used as a passive vibration isolation. The main aim of this study was to determine the damping properties of selected viscoelastic materials. In order to check the damping properties of tested materials, an experimental modal analysis, with the use of an electrodynamic vibration system, was performed. In this study, four kinds of specimens were considered. In the first step of the work, the beam made out of aluminum alloy was investigated. Afterwards, a cantilever beam was covered with a layer of bitumen-based material acting as a damper. This method is commonly known as a free layer damping treatment (FLD). In order to increase the damping capabilities, the previous configuration was improved by fixing a thin aluminum layer directly to the viscoelastic core. Such a treatment is called constrained layer damping (CLD). Subsequently, another polymer (butyl rubber) in the CLD configuration was tested for its damping properties. As a result of the performed experimental modal analysis, the frequencies of resonant vibrations and their corresponding amplitudes were obtained. The experimental results were used to quantitatively evaluate the damping properties of tested materials.
Electrochemical Impedance Spectroscopy Analysis of Organic Epoxy Coatings Reinforced with Nano Clay
Authors: Asperti Davide , Cabrini Marina , Lorenzi Sergio , Rosace Giuseppe , Omrani Abdollah , Pastore Tommaso
Published: June 2024

Electrochemical impedance spectroscopy (EIS) is a modern and efficient method for the evaluation of the protective abilities of coatings. However, the interpretation of the experimental data is a difficult task. This paper aims to investigate the effect of the addition of a nano clay, Cloesite 30B®, on the barrier properties of an epoxy-based system through electrochemical impedance spectroscopy in an aerated sodium chloride solution. The EIS spectra of the samples analysed showed different evolutions over time. The subsequent processing of spectra using equivalent electrical circuits is an excellent analytical tool and allows the protective capacity of coatings to be assessed. By using this analysis, it was possible to define and comprehend the impact of adding nano clay in different concentrations to the epoxy resin coating. The work has shown the effectiveness of increasing the barrier effect of the coating with this type of nano clay. However, the improvement is linked to obtaining a correct dispersion of nanoparticles. Otherwise, there is the formation of macro-clusters of particles inside the coating. Their appearance can cause a deterioration in coating performance.
The Influence of Heat Treatment on the Microstructure and Properties of a Cu-Bearing Ultra-Low Carbon Steel
Authors: Zhang Weina , Gao Zhanjie , Zhang Huimin , Wei Hao , Chen Zejin , Xue Wenying , Liu Zhenyu
Published: June 2024

This study reveals the relationship between the Cu precipitates and mechanical properties of a Cu-baring ultra-low carbon steel after two-phase zone quenching and tempering at 923 K for 0.5–2.5 h. The tensile and microstructural properties were investigated as a function of heat treatment time. The contribution of the precipitation-strengthening mechanism to yield strength was calculated. The size, morphology, and distribution of the precipitated particles were observed using TEM. As the heat treatment time increased, the strength gradually decreased and then remained stable, and the elongation gradually increased and then remained stable. Additionally, the contributions of each strengthening mechanism to the yield strength under different heat treatments were 117, 107, 102, and 89 MPa, respectively. The size and quantity of the precipitates increased with the increase in heat treatment time. After tempering for more than 2 h, the precipitates continued to coarsen, but their quantity decreased. The precipitated Cu had a 3R structure with a length of approximately 17.1 nm and a width of approximately 9.7 nm, with no twinning inside. The stacking order was ABC/ABC. The stable Cu precipitation structure was FCC, maintaining a K-S orientation relationship 11¯1FCC Cu //(0 1 1) α, 1¯10FCC Cu//[11¯1] α.
Oxidation Driven Damage on SiC/BN/SiC Ceramic Matrix Composite Aero-Engine Structures: An Iterative Computational Framework
Authors: Canale Giacomo , Citarella Roberto
Published: June 2024

Ceramic matrix composites (CMCs) could be a game changer in the aero-engine industry. Their density is circa one-third of their metallic counterpart. CMCs, furthermore, offer increased strength and greater capability at very high temperatures. This would allow for a reduction in cooling and an increased engine performance. Some challenges, besides the complexity of the manufacturing process, however, remain for the structural integrity of this technology. CMCs are inherently brittle; furthermore, they tend to oxidise when attacked by water or oxygen, and their constituents become brittle and more prone to failure. There are two main points of novelty proposed by this work. The first one is to model and reproduce recent oxidation experimental data with a simple Fick’s law implemented in Abaqus. The parameters of this modelling are a powerful tool for the design of such material systems. The second aspect consists in the development of a new computational framework for iteratively calculating oxygen diffusion and stiffness degradation of the material. Oxidation and stiffness degradation are in fact coupled phenomena. The crack (or microcracking) opening, the function of applied stress, accelerates oxygen diffusion whilst the oxidation diffusion itself contributes to embrittlement and then damage introduction in the material system.
Thermodynamic Model for Hydrogen Production from Rice Straw Supercritical Water Gasification
Authors: Liu Zhigang , Peng Zhiyong , Yi Lei , Wang Le , Chen Jingwei , Chen Bin , Guo Liejin
Published: June 2024

Supercritical water gasification (SCWG) technology is highly promising for its ability to cleanly and efficiently convert biomass to hydrogen. This paper developed a model for the gasification of rice straw in supercritical water (SCW) to predict the direction and limit of the reaction based on the Gibbs free energy minimization principle. The equilibrium distribution of rice straw gasification products was analyzed under a wide range of parameters including temperatures of 400–1200 °C, pressures of 20–50 MPa, and rice straw concentrations of 5–40 wt%. Coke may not be produced due to the excellent properties of supercritical water under thermodynamic constraints. Higher temperatures, lower pressures, and biomass concentrations facilitated the movement of the chemical equilibrium towards hydrogen production. The hydrogen yield was 47.17 mol/kg at a temperature of 650 °C, a pressure of 25 MPa, and a rice straw concentration of 5 wt%. Meanwhile, there is an absorptive process in the rice straw SCWG process for high-calorific value hydrogen production. Energy self-sufficiency of the SCWG process can be maintained by adding small amounts of oxygen (ER < 0.2). This work would be of great value in guiding rice straw SCWG experiments.
Study on Spline Stress of Separator Plates in a Wet Multi-Plate Clutch
Authors: Ma Biao , Chen Xiaobo , Zheng Changsong , Yu Liang , Zhao Qin , Lu Weichen
Published: June 2024

The spline teeth fracture of separator plates in wet multi-plate clutches compromises driving safety and the vehicle’s lifespan. Tooth fracture is mainly caused by stress concentration at the tooth root and uneven circumferential load distribution. This paper considers parameters such as torque, teeth count, tooth profile, and misalignment errors, establishing the corresponding finite element (FE) model to analyze the impact of the above-mentioned parameters on the strength of the separator plates. Analysis under even and biased load circumstances demonstrated that an optimum tooth count and profile can significantly increase the strength of the separator plates, offering advice for the optimized design of wet multi-plate clutch separator plates.
Recovery of Plastics from WEEE through Green Sink–Float Treatment
Authors: Fiorente Annarita , D’Agostino Germano , Petrella Andrea , Todaro Francesco , Notarnicola Michele
Published: June 2024

Increasing demand for electrical and electronic equipment results in the generation of a rapidly growing waste stream, known by the acronym WEEE (waste electrical and electronic equipment). The purpose of this study was to evaluate the effectiveness of green sink–float treatment in sorting plastic polymers typically found in WEEE (PP, ABS, PA6, PS, and PVC). Molasses, a by-product of sugar bio-refining, was added in various concentrations to water to form solutions at different densities. The methodology was initially tested on virgin polymers; later, it was applied to plastics from a WEEE treatment plant. The polymers were characterised through near infrared spectroscopy (NIRS) and Fourier-transform infrared spectroscopy (FTIRS) analyses; the detection of any additives and flame retardants was conducted using the sliding spark technology (SSS2) and scanning electron microscope (SEM—EDX). The results showed that, for plastics from WEEE, the recovery efficiency was 55.85% for PP in a solution of tap water while the remaining part of PP (44.15%) was recovered in a solution of water to which 90% molasses was added. Furthermore, 100% recovery efficiency was obtained for PS and 93.73% for ABS in a solution of tap water with the addition of 10% w/v molasses. A recovery efficiency of 100% was obtained for PVC and 100% for PA6 in a solution consisting solely of molasses.
Alternative Method for Determination of Vibroacoustic Material Parameters for Building Applications
Authors: Nering Krzysztof , Nering Konrad
Published: June 2024

The development of urbanization and the resulting expansion of residential and transport infrastructures pose new challenges related to ensuring comfort for city dwellers. The emission of transport vibrations and household noise reduces the quality of life in the city. To counteract this unfavorable phenomenon, vibration isolation is widely used to reduce the propagation of vibrations and noise. A proper selection of vibration isolation is necessary to ensure comfort. This selection can be made based on a deep understanding of the material parameters of the vibration isolation used. This mainly includes dynamic stiffness and damping. This article presents a comparison of the method for testing dynamic stiffness and damping using a single degree of freedom (SDOF) system and the method using image processing, which involves tracking the movement of a free-falling steel ball onto a sample of the tested material. Rubber granules, rubber granules with rubber fibers, and rebound polyurethanes were selected for testing. Strong correlations were found between the relative indentation and dynamic stiffness (at 10–60 MN/m3) and the relative rebound and damping (for 6–12%). Additionally, a very strong relationship was determined between the density and fraction of the critical damping factor/dynamic stiffness. The relative indentation and relative rebound measurement methods can be used as an alternative method to measure the dynamic stiffness and critical damping factor, respectively.
A Tripeptide (Ser-Arg-Pro, SRP) from Sipunculus nudus L. Improves Cadmium-Induced Acute Kidney Injury by Targeting the MAPK, Inflammatory, and Apoptosis Pathways in Mice
Authors: Pan Yanmei , Peng Zhilan , Fang Zhijia , Iddrisu Lukman , Sun Lijun , Deng Qi , Gooneratne Ravi
Published: June 2024

Cadmium (Cd) is a toxic heavy metal that causes nephrosis, including acute kidney injury. To prevent and treat acute kidney injury (AKI) following Cd exposure, a tripeptide, Ser-Arg-Pro (SRP), from Sipunculus nudus L. was employed, and its potential efficacy in AKI was assessed. Oral administration of SRP significantly alleviated Cd-induced kidney damage, leading to improved renal function and the attenuation of structural abnormalities. A network pharmacology analysis revealed the potential of SRP in renal protection by targeting various pathways, including mitogen-activated protein kinase (MAPK) signaling, inflammatory response, and apoptosis pathways. Mechanistic studies indicated that SRP achieves renal protection by inhibiting the activation of MAPK pathways (phosphorylation of p38, p56, ERK, and JNK) in the oxidative stress cascade, suppressing inflammatory responses (iNOS, Arg1, Cox2, TNF-α, IL-1β, and IL-6), and restoring altered apoptosis factors (caspase-9, caspase-3, Bax, and Bcl-2). Hence, SRP has the potential to be used as a therapeutic agent for the treatment of Cd-induced nephrotoxicity.
The Effects of Almond Consumption on Cardiovascular Health and Gut Microbiome: A Comprehensive Review
Authors: Singar Saiful , Kadyan Saurabh , Patoine Cole , Park Gwoncheol , Arjmandi Bahram , Nagpal Ravinder
Published: June 2024

The consumption of almonds has been associated with several health benefits, particularly concerning cardiovascular and intestinal health. In this comprehensive review, we compile and deliberate studies investigating the effects of almond consumption on cardiovascular disease (CVD) risk factors and gut health. Almonds are rich in monounsaturated fats, fiber, vitamins, minerals, and polyphenols, which contribute to their health-promoting properties. Regular intake of almonds has been shown to improve lipid profiles by reducing LDL cholesterol and enhancing HDL functionality. Additionally, almonds aid in glycemic control, blood pressure reduction, and chronic inflammation amelioration, which are critical for cardiovascular health. The antioxidant properties of almonds, primarily due to their high vitamin E content, help in reducing oxidative stress markers. Furthermore, almonds positively influence body composition by reducing body fat percentage and central adiposity and enhancing satiety, thus aiding in weight management. Herein, we also contemplate the emerging concept of the gut–heart axis, where almond consumption appears to modulate the gut microbiome, promoting the growth of beneficial bacteria and increasing short-chain fatty acid production, particularly butyrate. These effects collectively contribute to the anti-inflammatory and cardioprotective benefits of almonds. By encompassing these diverse aspects, we eventually provide a systematic and updated perspective on the multifaceted benefits of almond consumption for cardiovascular health and gut microbiome, corroborating their broader consideration in dietary guidelines and public health recommendations for CVD risk reduction.
Effects of Bifidobacterium longum 35624 in Children and Adolescents with Irritable Bowel Syndrome
Authors: Cruchet Muñoz Sylvia , Verbeke Palma Sandra , Lera Marqués Lydia , Espinosa Pizarro María , Malig Mechasqui Jacqueline , Sorensen Katy
Published: June 2024

Irritable bowel syndrome (IBS) and vitamin D deficiency are common among children in Latin America. Previous studies show that Bifidobacterium longum35624TM improves IBS symptoms in adults. This real-world, single-arm, open-label study conducted in Chile investigated the effects of B. longum 35624 (1 × 109 colony-forming units, 12 weeks) on gastrointestinal symptoms (adapted IBS severity scoring system [IBS-SSS]; adapted Questionnaire on Pediatric Gastrointestinal Symptoms [QPGS], and Bristol Stool Form Scale) in 64 children and adolescents (8–18 years) and explored the relationship with baseline vitamin D status. Improvements in all IBS-SSS domains and composite score were observed at week 6 and 12 (p < 0.0007 versus baseline), with 98.3% of participants experiencing numerical improvements in ≥3 domains. Clinically meaningful improvement was seen in 96.6% of participants. The distribution of IBS-SSS severity categories shifted from moderate/severe at baseline to mild/remission (p < 0.0001). Improvements were not maintained during the two-week washout. Low baseline serum vitamin D levels did not correlate to IBS severity or probiotic response. QPGS significantly decreased from baseline to week 6 (p = 0.0005) and 12 (p = 0.02). B. longum 35624 may improve IBS symptoms in children and adolescents, even those with vitamin D deficiency. A confirmatory randomized controlled trial and further exploration of probiotic response and vitamin D status are needed.
The Effect of Intradialytic Exercise Using Virtual Reality on the Body Composition of Patients with Chronic Kidney Disease
Authors: Romeu-Perales Marta , Segura-Ortí Eva , Cana-Poyatos Alicia , Toquero-Correa Marina , Benavent-Caballer Vicent , Pans-Alcaina Delia , García-Maset Rafael , García-Testal Alicia
Published: June 2024

Background: Individuals with chronic kidney disease (CKD) often experience reduced muscle strength and diminished health-related quality of life (HRQoL), and engaging in regular exercise may improve them. The aim of this study was to assess the effect of intradialytic exercise using non-immersive virtual reality (VR) on body composition of patients with CKD on hemodialysis (HD). Methods: This was a substudy in a clinical trial of intradialytic exercise intervention using a non-immersive VR game in which the patient interacted by moving the lower limbs. Body composition was determined by BCM Fresenius multifrequency stereoscopic bioimpedance. Body mass index (BMI), fat tissue index (FTI), lean tissue index (LTI), extracellular/intracellular water (EIW), and phase angle (PA) were recorded in 52 patients, 24 in the control group (CG) and 28 in the exercise group (EG). Results: Statistically significant differences were observed between both groups. The LTI increased in the EG while it decreased in the CG. The FTI and the EIW decreased in the EG compared to the increase observed in the CG. Conclusions: Intradialytic exercise using non-immersive VR was associated with an increase in LTI and a decrease in FTI of CKD patients on HD.
What Lithuanian First-Graders Eat: Results of a 15-Year Semi-Longitudinal, Cross-Sectional Surveillance Study
Authors: Petrauskiene Ausra , Daugelaite Silvija , Salomskiene Aurelija , Speckauskiene Vita
Published: June 2024

This article presents the dietary habits of Lithuanian first-grade (7–8-year-old) students over a 15-year surveillance period to understand the trends and changes in their nutrition patterns. The presented data were collected from three study rounds of the Lithuanian Growth Surveillance Study conducted between 2008 and 2023, with a total sample of 11,594 first-grade students from all 10 counties of Lithuania. The main findings reveal significant shifts in breakfast consumption, with an increase in daily breakfast intake observed over the surveillance period. Conversely, the consumption of cereal porridge showed a notable decrease, particularly in the frequency of consumption. Positive changes were noted in the consumption of vegetables and fresh fruits, indicating an improvement in dietary quality. Also, a concerning trend of declining consumption of certain nutritious food groups like fish and dairy products is identified, whereas the consumption of sugary beverages is low. These findings underscore the importance of ongoing efforts to promote healthier eating habits among school-age children in Lithuania. Addressing these trends requires a multifaceted approach involving education, policy changes, and community-based interventions to ensure the long-term health and well-being of children.