bluuebunny/crossref_metadata_2025 huggingface dataset:
Inside-out, antimicrobial resistance mediated by efflux pumps in clinical strains of Acinetobacter baumannii isolated from burn wound infections
Authors: Hernández-Durán Melissa , Colín-Castro Claudia Adriana , Fernández-Rodríguez Diana , Delgado Gabriela , Morales-Espinosa Rosario , Martínez-Zavaleta María Guadalupe , Shekhar Chandra , Ortíz-Álvarez Jossue , García-Contreras Rodolfo , Franco-Cendejas Rafael , López-Jácome Luis Esaú
Published: July 2024

Acinetobacter baumannii belongs to the ESKAPE group. It is classified as a critical priority group by the World Health Organization and a global concern on account of its capacity to acquire and develop resistance mechanisms to multiple antibiotics. Data from the United States indicates 500 deaths annually. Resistance mechanisms of this bacterium include enzymatic pathways such as ß-lactamases, carbapenemases, and aminoglycoside-modifying enzymes, decreased permeability, and overexpression of efflux pumps. A. baumannii has been demonstrated to possess efflux pumps, which are classified as members of the MATE family, RND and MFS superfamilies, and SMR transporters. The aim of our work was to assess the distribution of efflux pumps and their regulatory gene expression in clinical strains of A. baumannii isolated from burned patients.

Methods

From the Clinical Microbiology Laboratory at the Instituto Nacional de Rehabilitación Luis Guillermo Ibarra Ibarra collection in Mexico, 199 strains were selected. Antibiotics susceptibilities were performed by broth microdilutions to determine minimal inhibitory concentrations. Phenotypic assays with efflux pump inhibitors were conducted using carbonyl cyanide 3-chlorophenylhydrazone (CCCP) and phenylalanine-arginine ß-naphthylamide (PAßN) in conjunction with amikacin, ceftazidime, imipenem, meropenem and levofloxacin. A search was conducted for structural genes that are linked to efflux pumps, and the relative expression of the adeR, adeS, and adeL genes was analyzed.

Results

Among a total of 199 strains, 186 exhibited multidrug resistance (MDR). Fluoroquinolones demonstrated the highest resistance rates, while minocycline and amikacin displayed comparatively reduced resistance rates (1.5 and 28.1, respectively). The efflux activity of fluorquinolones exhibited the highest phenotypic detection (from 85 to 100%), while IMP demonstrated the lowest activity of 27% with PAßN and 43.3% with CCCP. Overexpression was observed in adeS and adeL, with adeR exhibiting overexpression.

Concluding that clinical strains of A. baumannii from our institution exhibited efflux pumps as one of the resistance mechanisms.
Diversity and spatial genetic structure of the common opossum (Didelphis marsupialis) in an urban matrix of Northwestern Colombia
Authors: Henao-Sáenz Carolina , Herrera-Pérez Juliana , Soto-Calderón Iván D.
Published: July 2024

The common opossum (Didelphis marsupialis) is a marsupial widely distributed in the Neotropics, where it lives in urbanized environments. The apparent scarcity and fragmentation of available habitat, and high rates of vehicle collisions, may represent barriers to dispersal. To assess the functional connectivity of this species and its potential use as a model in urban ecology, we evaluated the structure and genetic diversity of opossums in the metropolitan area of the Aburrá Valley (AMVA), Colombia, using sequences of the mitochondrial Cytochrome B (Cytb) gene and nine autosomal microsatellite loci. Although Cytb presented low levels of variation, microsatellite markers revealed high genetic diversity (He = 0.852 and Ho = 0.698). The geographic distribution of mitochondrial lineages and a spatial principal component analysis (sPCA) of microsatellites showed the absence of genetic structure and effective barriers to dispersal of opossums in the study area. This scenario suggests that hostile landscapes such as those in urban environments may be suitable enough to allow the effective connectivity of some species such as the common opossum and others with high dispersal ability in urbanized areas. Nonetheless, it is necessary to evaluate the impact of releases of rescued fauna made by the environmental authority on the population structure of urban opossums.
The Varied Role of Atmospheric Rivers in Arctic Snow Depth Variations
Authors: Li Haili , Ke Chang‐Qing , Shen Xiaoyi , Zhu Qinghui , Cai Yu , Luo Lanhua
Published: July 2024

The state and fate of snow on sea ice are crucial in the mass and energy balance of sea ice. The function of atmospheric rivers (ARs) on snow depth over sea ice has not been measured thus far, limiting the understanding of the mechanism of snow depth changes. Here, the effect of ARs on snow depth changes was explored. We found that increased AR frequency is responsible for winter‐autumn snow accumulation and spring‐summer snow melting. The 2 m air temperature (T2m), rainfall, snowfall, mean net longwave radiation (NLR), mean net shortwave radiation (NSR) and cloud radiative effect (CRE) during ARs explain the changes in snow depth triggered by AR occurrence. This work helps us understand how ARs affect snow depth changes through related physical processes, promotes an understanding of climate systems and provides a theoretical basis for snow treatment in sea ice models.
Reinterpreting ENSO's Role in Modulating Impactful Precipitation Events in California
Authors: Guirguis Kristen , Hatchett Benjamin , Gershunov Alexander , DeFlorio Michael , Clemesha Rachel , Brandt W. Tyler , Haleakala Kayden , Castellano Christopher , Luna Niño Rosa , Tardy Alexander , Anderson Michael , Ralph F. Martin
Published: July 2024

Water years (WY) 2017 and 2023 were anomalously wet for California, each alleviating multiyear drought. In both cases, this was unexpected given La Niña conditions, with most seasonal forecasts favoring drier‐than‐normal winters. We analyze over seven decades of precipitation and snow records along with mid‐tropospheric circulation to identify recurring weather patterns driving California precipitation and Sierra Nevada snowpack. Tropical forcing by ENSO causes subtle but important differences in these wet weather patterns, which largely drives the canonical seasonal ENSO‐precipitation relationship. However, the seasonal frequency of these weather patterns is not strongly modulated by ENSO and remains a primary source of uncertainty for seasonal forecasting. Seasonal frequency of ENSO‐independent weather patterns was a major cause of anomalous precipitation in WY2017, record‐setting snow in WY2023, and differences in precipitation outcome during recent El Niño winters 1983, 1998, and 2016. Improved understanding of recurrent atmospheric weather patterns could help to improve seasonal precipitation forecasts.
Broadband nonreciprocal thermal emissivity and absorptivity
Authors: Shayegan Komron J. , Hwang Jae S. , Zhao Bo , Raman Aaswath P. , Atwater Harry A.
Published: July 2024

A body that violates Kirchhoff’s law of thermal radiation exhibits an inequality in its spectral directional absorptivity and emissivity. Achieving such an inequality is of fundamental interest as well as a prerequisite for achieving thermodynamic limits in photonic energy conversion1 and radiative cooling2. Thus far, inequalities in the spectral directional emissivity and absorptivity have been limited to narrow spectral resonances3, or wavelengths well beyond the infrared regime4. Bridging the gap from basic demonstrations to practical applications requires control over a broad spectral range of the unequal spectral directional absorptivity and emissivity. In this work, we demonstrate broadband nonreciprocal thermal emissivity and absorptivity by measuring the thermal emissivity and absorptivity of gradient epsilon-near-zero InAs layers of subwavelength thicknesses (50 nm and 150 nm) with an external magnetic field. The effect occurs in a spectral range (12.5–16 μm) that overlaps with the infrared transparency window and is observed at moderate (1 T) magnetic fields.
CELF4 (rs1786814) gene polymorphism and speckle-tracking Echocardiography for cardiovascular complications in childhood cancer survivors
Authors: Ragab Seham M. , El-Hawy Mahmoud A. , El-Hefnawy Sally M. , El –Deeb Hend M. A. , Elfalah Amany S. , Mahmoud Asmaa A.
Published: July 2024

Background

Despite a well-known dose-dependent association between the risk of cardiac dysfunction and anthracycline, the risk of cardiac dysfunction for any given anthracycline dose varies between patients. So, we assessed CELF4 (rs1786814) gene polymorphism on anthracycline-related cardiotoxicity in childhood cancer survivors (CCS).

Methods

This comparative cross-sectional study included 53 CCS who had regular follow-up visits at the Pediatric Oncology Unit, Menoufia University Hospital. CELF4 (rs1786814) gene polymorphism and conventional and speckle-tracking Echocardiography were done for all survivors.

Results

Regarding CELF4 (rs1786814) genotypes, significant differences existed between the studied groups with a predominance of GG homozygous mutation. For Echocardiographic findings, the ejection fraction and end-systolic diameter compared to the control group, were significantly lower in the survivors group. Speckle- tracking Echocardiography showed a significant difference regarding (GLPS-A4C) and (GLPS-LAX), with no significant difference regarding (GLPS-A2C), (GLPS-Avg) and left atrium between the studied groups. Multivariate logistic regression analysis illustrated a statistically significant relation between cumulative anthracycline dose >300 mg/m2 and CLEF4 (rs1786814) genotypes (GG and GA) and the risk of cardiotoxicity with more significance in GG mutation.

Conclusion

Early detection of ventricular dysfunction in CCS with subclinical cardiotoxicity with regular follow-up is promising before the development of life-threatening complications.

Impact

Early detection of anthracycline-related cardiotoxicity in childhood cancer survivors (CCS) after finishing chemotherapy.

CLEF4 (rs1786814) GG variant is more significant in CCS exposed to high-dose anthracycline.

GLPS holds promise as an early predictor of late left ventricular dysfunction and subclinical cardiotoxicity in CCS.
Examining immune-inflammatory mechanisms of probiotic supplementation in depression: secondary findings from a randomized clinical trial
Authors: Sempach Lukas , Doll Jessica P. K. , Limbach Verena , Marzetta Flavia , Schaub Anna-Chiara , Schneider Else , Kettelhack Cedric , Mählmann Laura , Schweinfurth-Keck Nina , Ibberson Mark , Lang Undine E. , Schmidt André
Published: July 2024

We recently indicated that four-week probiotic supplementation significantly reduced depression along with microbial and neural changes in people with depression. Here we further elucidated the biological modes of action underlying the beneficial clinical effects of probiotics by focusing on immune-inflammatory processes. The analysis included a total of N = 43 participants with depression, from which N = 19 received the probiotic supplement and N = 24 received a placebo over four weeks, in addition to treatment as usual. Blood and saliva were collected at baseline, at post-intervention (week 4) and follow-up (week 8) to assess immune-inflammatory markers (IL-1β, IL-6, CRP, MIF), gut-related hormones (ghrelin, leptin), and a stress marker (cortisol). Furthermore, transcriptomic analyses were conducted to identify differentially expressed genes. Finally, we analyzed the associations between probiotic-induced clinical and immune-inflammatory changes. We observed a significant group x time interaction for the gut hormone ghrelin, indicative of an increase in the probiotics group. Additionally, the increase in ghrelin was correlated with the decrease in depressive symptoms in the probiotics group. Transcriptomic analyses identified 51 up- and 57 down-regulated genes, which were involved in functional pathways related to enhanced immune activity. We identified a probiotic-dependent upregulation of the genes ELANE, DEFA4 and OLFM4 associated to immune activation and ghrelin concentration. These results underscore the potential of probiotic supplementation to produce biological meaningful changes in immune activation in patients with depression. Further large-scale mechanistic trials are warranted to validate and extend our understanding of immune-inflammatory measures as potential biomarkers for stratification and treatment response in depression. Trial Registration: www.clinicaltrials.gov, identifier: NCT02957591.
Surface-binding molecular multipods strengthen the halide perovskite lattice and boost luminescence
Authors: Kim Dong-Hyeok , Woo Seung-Je , Huelmo Claudia Pereyra , Park Min-Ho , Schankler Aaron M. , Dai Zhenbang , Heo Jung-Min , Kim Sungjin , Reuveni Guy , Kang Sungsu , Kim Joo Sung , Yun Hyung Joong , Park Jinwoo , Park Jungwon , Yaffe Omer , Rappe Andrew M. , Lee Tae-Woo
Published: July 2024

Reducing the size of perovskite crystals to confine excitons and passivating surface defects has fueled a significant advance in the luminescence efficiency of perovskite light-emitting diodes (LEDs). However, the persistent gap between the optical limit of electroluminescence efficiency and the photoluminescence efficiency of colloidal perovskite nanocrystals (PeNCs) suggests that defect passivation alone is not sufficient to achieve highly efficient colloidal PeNC-LEDs. Here, we present a materials approach to controlling the dynamic nature of the perovskite surface. Our experimental and theoretical studies reveal that conjugated molecular multipods (CMMs) adsorb onto the perovskite surface by multipodal hydrogen bonding and van der Waals interactions, strengthening the near-surface perovskite lattice and reducing ionic fluctuations which are related to nonradiative recombination. The CMM treatment strengthens the perovskite lattice and suppresses its dynamic disorder, resulting in a near-unity photoluminescence quantum yield of PeNC films and a high external quantum efficiency (26.1%) of PeNC-LED with pure green emission that matches the Rec.2020 color standard for next-generation vivid displays.
Causal relevance of different blood pressure traits on risk of cardiovascular diseases: GWAS and Mendelian randomisation in 100,000 Chinese adults
Authors: Pozarickij Alfred , Gan Wei , Lin Kuang , Clarke Robert , Fairhurst-Hunter Zammy , Koido Masaru , Kanai Masahiro , Okada Yukinori , Kamatani Yoichiro , Bennett Derrick , Du Huaidong , Chen Yiping , Yang Ling , Avery Daniel , Guo Yu , Yu Min , Yu Canqing , Schmidt Valle Dan , Lv Jun , Chen Junshi , Peto Richard , Collins Rory , Li Liming , Chen Zhengming , Millwood Iona Y. , Walters Robin G. ,   China Kadoorie Biobank Collaborative Group
Published: July 2024

Elevated blood pressure (BP) is major risk factor for cardiovascular diseases (CVD). Genome-wide association studies (GWAS) conducted predominantly in populations of European ancestry have identified >2,000 BP-associated loci, but other ancestries have been less well-studied. We conducted GWAS of systolic, diastolic, pulse, and mean arterial BP in 100,453 Chinese adults. We identified 128 non-overlapping loci associated with one or more BP traits, including 74 newly-reported associations. Despite strong genetic correlations between populations, we identified appreciably higher heritability and larger variant effect sizes in Chinese compared with European or Japanese ancestry populations. Using instruments derived from these GWAS, multivariable Mendelian randomisation demonstrated that BP traits contribute differently to the causal associations of BP with CVD. In particular, only pulse pressure was independently causally associated with carotid plaque. These findings reinforce the need for studies in diverse populations to understand the genetic determinants of BP traits and their roles in disease risk.
Spatially and temporally probing distinctive glycerophospholipid alterations in Alzheimer’s disease mouse brain via high-resolution ion mobility-enabled sn-position resolved lipidomics
Authors: Xu Shuling , Zhu Zhijun , Delafield Daniel G. , Rigby Michael J. , Lu Gaoyuan , Braun Megan , Puglielli Luigi , Li Lingjun
Published: July 2024

Dysregulated glycerophospholipid (GP) metabolism in the brain is associated with the progression of neurodegenerative diseases including Alzheimer’s disease (AD). Routine liquid chromatography-mass spectrometry (LC-MS)-based large-scale lipidomic methods often fail to elucidate subtle yet important structural features such as sn-position, hindering the precise interrogation of GP molecules. Leveraging high-resolution demultiplexing (HRdm) ion mobility spectrometry (IMS), we develop a four-dimensional (4D) lipidomic strategy to resolve GP sn-position isomers. We further construct a comprehensive experimental 4D GP database of 498 GPs identified from the mouse brain and an in-depth extended 4D library of 2500 GPs predicted by machine learning, enabling automated profiling of GPs with detailed acyl chain sn-position assignment. Analyzing three mouse brain regions (hippocampus, cerebellum, and cortex), we successfully identify a total of 592 GPs including 130 pairs of sn-position isomers. Further temporal GPs analysis in the three functional brain regions illustrates their metabolic alterations in AD progression.
Nondestructive flash cathode recycling
Authors: Chen Weiyin , Cheng Yi , Chen Jinhang , Bets Ksenia V. , Salvatierra Rodrigo V. , Ge Chang , Li John Tianci , Luong Duy Xuan , Kittrell Carter , Wang Zicheng , McHugh Emily A. , Gao Guanhui , Deng Bing , Han Yimo , Yakobson Boris I. , Tour James M.
Published: July 2024

Effective recycling of end-of-life Li-ion batteries (LIBs) is essential due to continuous accumulation of battery waste and gradual depletion of battery metal resources. The present closed-loop solutions include destructive conversion to metal compounds, by destroying the entire three-dimensional morphology of the cathode through continuous thermal treatment or harsh wet extraction methods, and direct regeneration by lithium replenishment. Here, we report a solvent- and water-free flash Joule heating (FJH) method combined with magnetic separation to restore fresh cathodes from waste cathodes, followed by solid-state relithiation. The entire process is called flash recycling. This FJH method exhibits the merits of milliseconds of duration and high battery metal recovery yields of ~98%. After FJH, the cathodes reveal intact core structures with hierarchical features, implying the feasibility of their reconstituting into new cathodes. Relithiated cathodes are further used in LIBs, and show good electrochemical performance, comparable to new commercial counterparts. Life-cycle-analysis highlights that flash recycling has higher environmental and economic benefits over traditional destructive recycling processes.
NMNAT2 is a druggable target to drive neuronal NAD production
Authors: Tribble James R. , Jöe Melissa , Varricchio Carmine , Otmani Amin , Canovai Alessio , Habchi Baninia , Daskalakis Evangelia , Chaleckis Romanas , Loreto Andrea , Gilley Jonathan , Wheelock Craig E. , Jóhannesson Gauti , Wong Raymond C. B. , Coleman Michael P. , Brancale Andrea , Williams Pete A.
Published: July 2024

Abstract

Maintenance of NAD pools is critical for neuronal survival. The capacity to maintain NAD pools declines in neurodegenerative disease. We identify that low NMNAT2, the critical neuronal NAD producing enzyme, drives retinal susceptibility to neurodegenerative insults. As proof of concept, gene therapy over-expressing full length human NMNAT2 is neuroprotective. To pharmacologically target NMNAT2, we identify that epigallocatechin gallate (EGCG) can drive NAD production in neurons through an NMNAT2 and NMN dependent mechanism. We confirm this by pharmacological and genetic inhibition of the NAD-salvage pathway. EGCG is neuroprotective in rodent (mixed sex) and human models of retinal neurodegeneration. As EGCG has poor drug-like qualities, we use it as a tool compound to generate novel small molecules which drive neuronal NAD production and provide neuroprotection. This class of NMNAT2 targeted small molecules could have an important therapeutic impact for neurodegenerative disease following further drug development.
Direct synthesis of partially ethoxylated branched polyethylenimine from ethanolamine
Authors: Brodie Claire N. , Goodfellow Alister S. , Andrews Matthew J. , Owen Aniekan E. , Bühl Michael , Kumar Amit
Published: July 2024

We report here a method to make a branched and partially ethoxylated polyethyleneimine derivative directly from ethanolamine. The polymerization reaction is catalysed by a pincer complex of Earth-abundant metal, manganese, and produces water as the only byproduct. Industrial processes to produce polyethyleneimines involve the transformation of ethanolamine to a highly toxic chemical, aziridine, by an energy-intensive/waste-generating process followed by the ring-opening polymerization of aziridine. The reported method bypasses the need to produce a highly toxic intermediate and presents advantages over the current state-of-the-art. We propose that the polymerization process follows a hydrogen borrowing pathway that involves (a) dehydrogenation of ethanolamine to form 2-aminoacetaldehyde, (b) dehydrative coupling of 2-aminoacetaldehyde with ethanolamine to form an imine derivative, and (c) subsequent hydrogenation of imine derivative to form alkylated amines.
Archaean multi-stage magmatic underplating drove formation of continental nuclei in the North China Craton
Authors: Liu Jin , Palin Richard M. , Mitchell Ross N. , Liu Zhenghong , Zhang Jian , Li Zhongshui , Cheng Changquan , Zhang Hongxiang
Published: July 2024

The geodynamic processes that formed Earth’s earliest continents are intensely debated. Particularly, the transformation from ancient crustal nuclei into mature Archaean cratons is unclear, primarily owing to the paucity of well-preserved Eoarchaean–Palaeoarchaean ‘protocrust’. Here, we report a newly identified Palaeoarchaean continental fragment—the Baishanhu nucleus—in northeastern North China Craton. U–Pb geochronology shows that this nucleus preserves five major magmatic events during 3.6–2.5 Ga. Geochemistry and zircon Lu–Hf isotopes reveal ancient 4.2–3.8 Ga mantle extraction ages, as well as later intraplate crustal reworking. Crustal architecture and zircon Hf–O isotopes indicate that proto-North China first formed in a stagnant/squishy lid geodynamic regime characterised by plume-related magmatic underplating. Such cratonic growth and maturation were prerequisites for the emergence of plate tectonics. Finally, these data suggest that North China was part of the Sclavia supercraton and that the Archaean onset of subduction occurred asynchronously worldwide.
Loss-of-function mutations in Keratin 32 gene disrupt skin immune homeostasis in pityriasis rubra pilaris
Authors: Shi Peidian , Chen Wenjie , Lyu Xinxing , Wang Zhenzhen , Li Wenchao , Jia Fengming , Zheng Chunzhi , Liu Tingting , Wang Chuan , Zhang Yuan , Mi Zihao , Sun Yonghu , Chen Xuechao , Chen Shengli , Zhou Guizhi , Liu Yongxia , Lin Yingjie , Bai Fuxiang , Sun Qing , Ogese Monday O. , Yu Qiang , Liu Jianjun , Liu Hong , Zhang Furen
Published: July 2024

Pityriasis rubra pilaris (PRP) is an inflammatory papulosquamous dermatosis, characterized by hyperkeratotic follicular papules and erythematous desquamative plaques. The precise pathogenic mechanism underlying PRP remains incompletely understood. Herein, we conduct a case-control study involving a cohort of 102 patients with sporadic PRP and 800 healthy controls of Han Chinese population and identify significant associations (P = 1.73 × 10−6) between PRP and heterozygous mutations in the Keratin 32 gene (KRT32). KRT32 is found to be predominantly localized in basal keratinocytes and exhibits an inhibitory effect on skin inflammation by antagonizing the NF-κB pathway. Mechanistically, KRT32 binds to NEMO, promoting excessive K48-linked polyubiquitination and NEMO degradation, which hinders IKK complex formation. Conversely, loss-of-function mutations in KRT32 among PRP patients result in NF-κB hyperactivation. Importantly, Krt32 knockout mice exhibit a PRP-like dermatitis phenotype, suggesting compromised anti-inflammatory function of keratinocytes in response to external pro-inflammatory stimuli. This study proposes a role for KRT32 in regulating inflammatory immune responses, with damaging variants in KRT32 being an important driver in PRP development. These findings offer insights into the regulation of skin immune homeostasis by keratin and open up the possibility of using KRT32 as a therapeutic target for PRP.
Reconfigurable metamaterial processing units that solve arbitrary linear calculus equations
Authors: Fu Pengyu , Xu Zimeng , Zhou Tiankuang , Li Hao , Wu Jiamin , Dai Qionghai , Li Yue
Published: July 2024

Calculus equations serve as fundamental frameworks in mathematics, enabling describing an extensive range of natural phenomena and scientific principles, such as thermodynamics and electromagnetics. Analog computing with electromagnetic waves presents an intriguing opportunity to solve calculus equations with unparalleled speed, while facing an inevitable tradeoff in computing density and equation reconfigurability. Here, we propose a reconfigurable metamaterial processing unit (MPU) that solves arbitrary linear calculus equations at a very fast speed. Subwavelength kernels based on inverse-designed pixel metamaterials are used to perform calculus operations on time-domain signals. In addition, feedback mechanisms and reconfigurable components are used to formulate and solve calculus equations with different orders and coefficients. A prototype of this MPU with a compact planar size of 0.93λ0×0.93λ0 (λ0 is the free-space wavelength) is constructed and evaluated in microwave frequencies. Experimental results demonstrate the MPU’s ability to successfully solve arbitrary linear calculus equations. With the merits of compactness, easy integration, reconfigurability, and reusability, the proposed MPU provides a potential route for integrated analog computing with high speed of signal processing.
Adaptative machine vision with microsecond-level accurate perception beyond human retina
Authors: Li Ling , Li Shasha , Wang Wenhai , Zhang Jielian , Sun Yiming , Deng Qunrui , Zheng Tao , Lu Jianting , Gao Wei , Yang Mengmeng , Wang Hanyu , Pan Yuan , Liu Xueting , Yang Yani , Li Jingbo , Huo Nengjie
Published: July 2024

Visual adaptive devices have potential to simplify circuits and algorithms in machine vision systems to adapt and perceive images with varying brightness levels, which is however limited by sluggish adaptation process. Here, the avalanche tuning as feedforward inhibition in bionic two-dimensional (2D) transistor is proposed for fast and high-frequency visual adaptation behavior with microsecond-level accurate perception, the adaptation speed is over 104 times faster than that of human retina and reported bionic sensors. As light intensity changes, the bionic transistor spontaneously switches between avalanche and photoconductive effect, varying responsivity in both magnitude and sign (from 7.6 × 104 to −1 × 103 A/W), thereby achieving ultra-fast scotopic and photopic adaptation process of 108 and 268 μs, respectively. By further combining convolutional neural networks with avalanche-tuned bionic transistor, an adaptative machine vision is achieved with remarkable microsecond-level rapid adaptation capabilities and robust image recognition with over 98% precision in both dim and bright conditions.
An in situ exploration of how Fe/N/C oxygen reduction catalysts evolve during synthesis under pyrolytic conditions
Authors: Yin Shuhu , Yi Hongyuan , Liu Mengli , Yang Jian , Yang Shuangli , Zhang Bin-Wei , Chen Long , Cheng Xiaoyang , Huang Huan , Huang Rui , Jiang Yanxia , Liao Honggang , Sun Shigang
Published: July 2024

In pursuing cheap and effective oxygen reduction catalysts, the Fe/N/C system emerges as a promising candidate. Nevertheless, the structural transformations of starting materials into Fe- and N-doped carbon catalysts remains poorly characterized under pyrolytic conditions. Here, we explore the evolution of Fe species and track the formation of Fe–N4 site development by employing diverse in-situ diagnostic techniques. In-situ heating microscopy reveals the initial formation of FeOx nanoparticles and subsequent internal migration within the carbon matrix, which stops once FeOx is fully reduced. The migration and decomposition of nanoparticles then leads to carbon layer reconstruction. Experimental and theoretical analysis reveals size-dependent behavior of FeOx where nanoparticles below 7 nm readily release Fe atoms to form Fe–N4 while nanoparticles with sizes >10 nm tend to coalesce and impede Fe–N4 site formation. The work visualizes the pyrolysis process of Fe/N/C materials, providing theoretical guidance for the rational design of catalysts.
Macroscopic homochiral helicoids self-assembled via screw dislocations
Authors: Wu Shengfu , Song Xin , Du Cong , Liu Minghua
Published: July 2024

Chirality is a fundamental property in nature and is widely observed at hierarchical scales from subatomic, molecular, supramolecular to macroscopic and even galaxy. However, the transmission of chirality across different length scales and the expression of homochiral nano/microstructures remain challenging. Herein, we report the formation of macroscopic homochiral helicoids with ten micrometers from enantiomeric pyromellitic diimide-based molecular triangle (PMDI-Δ) and achiral pyrene via a screw dislocation-driven co-self-assembly. Chiral transfer and expression from molecular and supramolecular levels, to the macroscopic helicoids, is continuous and follows the molecular chirality of PMDI-Δ. Furthermore, the screw dislocation and chirality transfer lead to a unidirectional curvature of the helicoids, which exhibit excellent circularly polarized luminescence with large |glum| values up to 0.05. Our results demonstrate the formation of a homochiral macroscopic organic helicoid and function emergence from small molecules via screw dislocations, which deepens our understanding of chiral transfer and expression across different length scales.
Measuring the burden of hundreds of BioBricks defines an evolutionary limit on constructability in synthetic biology
Authors: Radde Noor , Mortensen Genevieve A. , Bhat Diya , Shah Shireen , Clements Joseph J. , Leonard Sean P. , McGuffie Matthew J. , Mishler Dennis M. , Barrick Jeffrey E.
Published: July 2024

Engineered DNA will slow the growth of a host cell if it redirects limiting resources or otherwise interferes with homeostasis. Escape mutants that alleviate this burden can rapidly evolve and take over cell populations, making genetic engineering less reliable and predictable. Synthetic biologists often use genetic parts encoded on plasmids, but their burden is rarely characterized. We measured how 301 BioBrick plasmids affected Escherichia coli growth and found that 59 (19.6%) were burdensome, primarily because they depleted the limited gene expression resources of host cells. Overall, no BioBricks reduced the growth rate of E. coli by >45%, which agreed with a population genetic model that predicts such plasmids should be unclonable. We made this model available online for education (https://barricklab.org/burden-model) and added our burden measurements to the iGEM Registry. Our results establish a fundamental limit on what DNA constructs and genetic modifications can be successfully engineered into cells.
Enabling long-distance hydrogen spillover in nonreducible metal-organic frameworks for catalytic reaction
Authors: Bai Xiao-Jue , Yang Caoyu , Tang Zhiyong
Published: July 2024

Hydrogen spillover is an extraordinary effect in heterogeneous catalysis and hydrogen storage, which refers to the surface migration of metal particle-activated hydrogen atoms over the solid supports. Historical studies on this phenomenon have mostly been limited to reducible metal oxides where the long-distance proton-electron coupled migration mechanism has been established, yet the key question remains on how to surmount short-distance and defect-dependent hydrogen migration on nonreducible supports. By demerging hydrogen migration and hydrogenation reaction, here we demonstrate that the hydrogen spillover in nonreducible metal-organic frameworks (MOFs) can be finely modulated by the ligand functional groups or embedded water molecules, enabling significant long-distance (exceed 50 nm) movement of activated hydrogen. Furthermore, using sandwich nanostructured MOFs@Pt@MOFs as catalysts, we achieve highly selective hydrogenation of N-heteroarenes via controllable hydrogen spillover from Pt to MOFs-shell. We anticipate that this work will enhance the understanding of hydrogen spillover and shed light on de novo design of MOFs supported catalysts for many important reactions involving hydrogen.
A solar-driven atmospheric water extractor for off-grid freshwater generation and irrigation
Authors: Yang Kaijie , Pan Tingting , Ferhat Nadia , Felix Alejandra Ibarra , Waller Rebekah E. , Hong Pei-Ying , Vrouwenvelder Johannes S. , Gan Qiaoqiang , Han Yu
Published: July 2024

Solar-driven atmospheric water extraction (SAWE) is a sustainable technology for decentralized freshwater supply. However, most SAWE systems produce water intermittently due to the cyclic nature, with adoption hindered by complex design requirements or periodic manual operations. Herein, a fully passive SAWE system that can continuously produce freshwater under sunlight is presented. By optimizing the three-dimensional architecture to facilitate spontaneous mass transport and efficient energy utilization, this system can consistently produce 0.65 L m−2 h−1 of freshwater under 1-sun illumination at 90% relative humidity (RH) and functions in arid environments with an RH as low as 40%. We test the practical performance of a scaled-up system in Thuwal, Saudi Arabia over 35 days across two seasons. The system produces 2.0–3.0 L m−2 per day of freshwater during the summer and 1.0–2.8 L m−2 per day of freshwater during the fall, without requiring additional maintenance. Intriguingly, we demonstrate the system’s potential for off-grid irrigation by successfully growing cabbage plants using atmospheric water. This passive SAWE system, harnessing solar energy to continuously extract moisture from air for drinking and irrigation, offers a promising solution to address the intertwined challenges of energy, water, and food supply, particularly for remote and water-scarce regions.
GmAMT2.1/2.2-dependent ammonium nitrogen and metabolites shape rhizosphere microbiome assembly to mitigate cadmium toxicity
Authors: Cai Zhandong , Yu Taobing , Tan Weiyi , Zhou Qianghua , Liu Lingrui , Nian Hai , Lian Tengxiang
Published: July 2024

Cadmium (Cd), a heavy metal, is negatively associated with plant growth. AMT (ammonium transporter) genes can confer Cd resistance and enhance nitrogen (N) uptake in soybeans. The potential of AMT genes to alleviate Cd toxicity by modulating rhizosphere microbiota remains unkonwn. Here, the rhizosphere microbial taxonomic and metabolic differences in three genotypes, i.e., double knockout and overexpression lines and wild type, were identified. The results showed that GmAMT2.1/2.2 genes could induce soybean to recruit beneficial microorganisms, such as Tumebacillus, Alicyclobacillus, and Penicillium, by altering metabolites. The bacterial, fungal, and cross-kingdom synthetic microbial communities (SynComs) formed by these microorganisms can help soybean resist Cd toxicity. The mechanisms by which SynComs help soybeans resist Cd stress include reducing Cd content, increasing ammonium (NH4+-N) uptake and regulating specific functional genes in soybeans. Overall, this study provides valuable insights for the developing microbial formulations that enhance Cd resistance in sustainable agriculture.
Asymmetries in the Southern Ocean contribution to global heat and carbon uptake
Authors: Williams Richard G. , Meijers Andrew J. S. , Roussenov Vassil M. , Katavouta Anna , Ceppi Paulo , Rosser Jonathan P. , Salvi Pietro
Published: July 2024

The Southern Ocean provides dominant contributions to global ocean heat and carbon uptake, which is widely interpreted as resulting from its unique upwelling and circulation. Here we show a large asymmetry in these contributions, with the Southern Ocean accounting for 83 ± 33% of global heat uptake versus 43 ± 3% of global ocean carbon uptake over the historical period in state-of-the-art climate models. Using single radiative forcing experiments, we demonstrate that this historical asymmetry is due to suppressed heat uptake by northern oceans from enhanced aerosol forcing. In future projections, such as SSP2-4.5 where greenhouse gases increasingly dominate radiative forcing, the Southern Ocean contributions to global heat and carbon uptake become more comparable, 52 ± 5% and 47 ± 4%, respectively. Hence, the past is not a reliable indicator of the future, with the northern oceans becoming important for heat uptake while the Southern Ocean remains important for both heat and carbon uptake.
Bilateral flows and rates of international migration of scholars for 210 countries for the period 1998-2020
Authors: Akbaritabar Aliakbar , Theile Tom , Zagheni Emilio
Published: July 2024

A lack of comprehensive migration data is a major barrier for understanding the causes and consequences of migration processes, including for specific groups like high-skilled migrants. We leverage large-scale bibliometric data from Scopus and OpenAlex to trace the global movements of scholars. Based on our empirical validations, we develop pre-processing steps and offer best practices for the measurement and identification of migration events. We have prepared a publicly accessible dataset that shows a high level of correlation between the counts of scholars in Scopus and OpenAlex for most countries. Although OpenAlex has more extensive coverage of non-Western countries, the highest correlations with Scopus are observed in Western countries. We share aggregated yearly estimates of international migration rates and of bilateral flows for 210 countries and areas worldwide for the period 1998–2020 and describe the data structure and usage notes. We expect that the publicly shared dataset will enable researchers to further study the causes and the consequences of migration of scholars to forecast the future mobility of academic talent worldwide.
Potential for spatial coexistence of a transboundary migratory species and wind energy development
Authors: Huang Ta-Ken , Feng Xiao , Derbridge Jonathan J. , Libby Kaitlin , Diffendorfer Jay E. , Thogmartin Wayne E. , McCracken Gary , Medellin Rodrigo , López-Hoffman Laura
Published: July 2024

Global expansion in wind energy development is a notable achievement of the international community’s effort to reduce carbon emissions during energy production. However, the increasing number of wind turbines have unintended consequences for migratory birds and bats. Wind turbine curtailment and other mitigation strategies can reduce fatalities, but improved spatial and temporal data are needed to identify the most effective way for wind energy development and volant migratory species to coexist. Mexican free-tailed bats (Tadarida brasiliensis mexicana) account for a large proportion of known bat fatalities at wind facilities in the southwestern US. We examined the geographic concordance between existing wind energy generation facilities, areas of high wind potential amenable for future deployment of wind facilities, and seasonally suitable habitat for these bats. We used ecological niche modeling to determine species distribution during each of 4 seasons. We used a multi-criteria GIS-based approach to produce a wind turbine siting suitability map. We identified seasonal locations with highest and lowest potential for the species’ probability of occurrence, providing a potential explanation for the higher observed fatalities during fall migration. Thirty percent of 33,606 wind turbines within the southwestern US occurred in highly suitable areas for Mexican free-tailed bats, primarily in west Texas. There is also broad spatial overlap between areas of high wind potential and areas of suitable habitat for Mexican free-tailed bats. Because of this high degree of overlap, our results indicate that post-construction strategies, such as curtailing the timing of operations and deterrents, would be more effective for bat conservation than strategic siting of new wind energy installations.
Intergenerational effects of racism on amygdala and hippocampus resting state functional connectivity
Authors: Kral T. R. A. , Williams C. Y. , Wylie A. C. , McLaughlin K. , Stephens R. L. , Mills-Koonce W. R. , Birn R. M. , Propper C. B. , Short S. J.
Published: July 2024

Racism is an insidious problem with far-reaching effects on the lives of Black, Indigenous, and People of Color (BIPOC). The pervasive negative impact of racism on mental health is well documented. However, less is known about the potential downstream impacts of maternal experiences of racism on offspring neurodevelopment. This study sought to examine evidence for a biological pathway of intergenerational transmission of racism-related trauma. This study examined the effects of self-reported maternal experiences of racism on resting state functional connectivity (rsFC) in n = 25 neonates (13 female, 12 male) birthed by BIPOC mothers. Amygdala and hippocampus are brain regions involved in fear, memory, and anxiety, and are central nodes in brain networks associated with trauma-related change. We used average scores on the Experiences of Racism Scale as a continuous, voxel-wise regressor in seed-based, whole-brain connectivity analysis of anatomically defined amygdala and hippocampus seed regions of interest. All analyses controlled for infant sex and gestational age at the 2-week scanning session. More maternal racism-related experiences were associated with (1) stronger right amygdala rsFC with visual cortex and thalamus; and (2) stronger hippocampus rsFC with visual cortex and a temporo-parietal network, in neonates. The results of this research have implications for understanding how maternal experiences of racism may alter neurodevelopment, and for related social policy.
Rapid differentiation of cystic fibrosis-related bacteria via reagentless atmospheric pressure photoionisation mass spectrometry
Authors: Haworth-Duff Adam , Smith Barry L. , Sham Tung-Ting , Boisdon Cedric , Loughnane Paul , Burnley Mark , Hawcutt Daniel B. , Raval Rasmita , Maher Simon
Published: July 2024

Breath analysis is an area of significant interest in medical research as it allows for non-invasive sampling with exceptional potential for disease monitoring and diagnosis. Volatile organic compounds (VOCs) found in breath can offer critical insight into a person’s lifestyle and/or disease/health state. To this end, the development of a rapid, sensitive, cost-effective and potentially portable method for the detection of key compounds in breath would mark a significant advancement. Herein, we have designed, built and tested a novel reagent-less atmospheric pressure photoionisation (APPI) source, coupled with mass spectrometry (MS), utilising a bespoke bias electrode within a custom 3D printed sampling chamber for direct analysis of VOCs. Optimal APPI-MS conditions were identified, including bias voltage, cone voltage and vaporisation temperature. Calibration curves were produced for ethanol, acetone, 2-butanone, ethyl acetate and eucalyptol, yielding R2 > 0.99 and limits of detection < 10 pg. As a pre-clinical proof of concept, this method was applied to bacterial headspace samples ofEscherichia coli(EC),Pseudomonas aeruginosa(PSA) andStaphylococcus aureus(SA) collected in 1 L Tedlar bags. In particular, PSA and SA are commonly associated with lung infection in cystic fibrosis patients. The headspace samples were classified using principal component analysis with 86.9% of the total variance across the first three components and yielding 100% classification in a blind-sample study. All experiments conducted with the novel APPI arrangement were carried out directly in real-time with low-resolution MS, which opens up exciting possibilities in the future for on-site (e.g., in the clinic) analysis with a portable system.
Reliable prediction of implant size and axial alignment in AI-based 3D preoperative planning for total knee arthroplasty
Authors: Lan Qing , Li Shulin , Zhang Jiahao , Guo Huiling , Yan Laipeng , Tang Faqiang
Published: July 2024

The size and axial alignment of prostheses, when planned during total knee replacement (TKA) are critical for recovery of knee function and improvement of knee pain symptoms. This research aims to study the effect of artificial intelligence (AI)-based preoperative three dimensional (3D) planning technology on prosthesis size and axial alignment planning in TKA, and to compare its advantages with two dimensional (2D) X-ray template measurement technology. A total of 60 patients with knee osteoarthritis (KOA) who underwent TKA for the first time were included in the AI (n = 30) and 2D (n = 30) groups. The preoperative and postoperative prosthesis size, femoral valgus correction angle (VCA) and hip-knee-ankle angle (HKA) were recorded and compared between the two groups. The results of the University of Western Ontario and McMaster University Osteoarthritis Index (WOMAC) and the American Knee Association Score (AKS) were evaluated before surgery, 3 months, 6 months, and 12 months after surgery. The accuracy of prosthesis size, VCA and HKA prediction in AI group was significantly higher than that in 2D group (P < 0.05). The WOMAC and AKS scores in AI group at 3 months, 6 months and 12 months after surgery were better than those in 2D group (P < 0.05). Both groups showed significant improvement in WOMAC and AKS scores at 12 months follow-up. AI-based preoperative 3D planning technique has more reliable planning effect for prosthesis size and axial alignment in TKA.
Distribution and soil threshold of selenium in the cropland of southwest mountainous areas in China
Authors: Wang Sheng , Liu Qi , Liu Zhizong , Chen Wen , Zhao Xuanyue , Zhang Jilai , Bao Li , Zhang Naiming
Published: July 2024

To investigate the distribution characteristics of selenium (Se) in mountainous soil-crop systems and examine the threshold value of Se-rich soil, 275 soil samples and 153 associated crop samples (rice, maize, tea, nuts, konjac, mushrooms, buckwheat, and coffee) were collected in Ximeng County, a typical mountainous area in southwest China. The total Se, available Se, organic matter, pH, sampling point elevation, and crop Se content were analyzed to examine the distribution characteristics of soil Se and the ability of primary crops to enrich Se in Ximeng County. Random forest and multiple regression models were established to identify the factors influencing the available soil Se and the crop Se enrichment coefficient. Finally, the Se-rich soil threshold was examined based on the total Se, available Se, and Se content in primary crops (rice, maize, and tea). The results showed soil Se resource abundance in the study region, with high Se soil accounting for 64.72% of the entire area. The soil Se content displayed significant spatial autocorrelation. The average Se enrichment coefficient of the main cultivated crops included mushrooms > nuts > rice > coffee > tea > maize > buckwheat > konjac. The total Se content in the soil had the highest impact on the available Se content in the soil and the Se enrichment coefficient of crops. A Se-rich soil threshold of 0.3 mg·kg−1 was used for rice and maize, while that of tea was 0.4 mg·kg−1. This result provided a theoretical basis for developing and utilizing Se resources in mountainous soil in southwestern China and dividing the Se-rich soil threshold.