bluuebunny/crossref_metadata_2025 huggingface dataset:
βγ G-proteins, but not regulators of G-protein signaling 4, modulate opioid-induced respiratory rate depression
Authors: Danaf Jamil , da Silveira Scarpellini Carolina , Montandon Gaspard
Published: April 2023

Opioid medications are the mainstay of pain management but present substantial side-effects such as respiratory depression which can be lethal with overdose. Most opioid drugs, such as fentanyl, act on opioid receptors such as the G-protein-coupled µ-opioid receptors (MOR). G-protein-coupled receptors activate pertussis toxin-sensitive G-proteins to inhibit neuronal activity. Binding of opioid ligands to MOR and subsequent activation G proteins βγ is modulated by regulator of G-protein signaling (RGS). The roles of G-proteins βγ and RGS in MOR-mediated inhibition of the respiratory network are not known. Using rodent models to pharmacologically modulate G-protein signaling, we aim to determine the roles of βγ G-proteins and RGS4. We showed that inhibition of βγ G-proteins using gallein perfused in the brainstem circuits regulating respiratory depression by opioid drugs results in complete reversal of respiratory depression. Blocking of RGS4 using CCG55014 did not change the respiratory depression induced by MOR activation despite co-expression of RGS4 and MORs in the brainstem. Our results suggest that neuronal inhibition by opioid drugs is mediated by G-proteins, but not by RGS4, which supports the concept that βγ G-proteins could be molecular targets to develop opioid overdose antidotes without the risks of re-narcotization often found with highly potent opioid drugs. On the other hand, RGS4 mediates opioid analgesia, but not respiratory depression, and RGS4 may be molecular targets to develop pain therapies without respiratory liability.
Evolutionary algorithm for the optimization of meal intake and insulin administration in patients with type 2 diabetes
Authors: Gonzalez-Flo Eva , Kheirabadi Elaheh , Rodriguez-Caso Carlos , Macía Javier
Published: April 2023

The optimal management of type 2 diabetes (T2DM) is complex and involves an appropriate combination of diet, exercise, and different pharmacological treatments. Artificial intelligence-based tools have been shown to be very useful for the diagnosis and treatment of diverse pathologies, including diabetes. In the present study, we present a proof of concept of the potential of an evolutionary algorithm to optimize the meal size, timing and insulin dose for the control of glycemia. We found that an appropriate distribution of food intake throughout the day permits a reduction in the insulin dose required to maintain glycemia within the range recommended by the American Diabetes Association for patients with T2DM of a range of severities. Furthermore, the effects of restrictions to both the timing and amount of food ingested were assessed, and we found that an increase in the amount of insulin was required to control glycemia as dietary intake became more restricted. In the near future, the use of these computational tools should permit patients with T2DM to optimize their personal meal schedule and insulin dose, according to the severity of their diabetes.
Kinematic parameters and metabolic power in elite soccer players: A small sided a large sided games comparison
Authors: Zlojutro Nemanja , Eler Serdar , Joksimovic Marko , Eler Nebahat , Marković Saša , Kukrić Aleksandar , Goranovic Kosta
Published: April 2023

Introduction: The goal of this paper is to determine what happens in one minute (on average) in kinematic parameters and metabolic power in small sided games (SSG) (3v3; 5v5) and large sided games (LSG) (10v10) and in which games kinematic parameters and metabolic power are best developed.

Methods: The participants of this study were 22 professional football players, height 182.95±6.52 cm, mass 77.17±8.21 kg, body mass index (BMI) 22.97±1.47 kg/m2, body fat 9.85±2.55 %, aged 27.1±5.4 yrs, who played in the Premier League of Bosnia and Herzegovina. Data total distance (TD), maximum speed (MS), number of accelerations (nAcc), number of decelerations (nDec), number of sprints (nS), high intensity distance (Z4≥19.8 km/h), sprint distance (Z5≥25.2 km/h) and movements requiring a certain metabolic power (Pmet), were collected using a 20 Hz Global positioning system (GPS) system Pro2 (GPEXE, Exelio srl, Udine, Italy), on a total of 307 individual observations.

Results: The results showed that the average total distance was significantly higher in the 5v5 (135.16±18.78 m) and 10v10 (133.43±20.06 m) games (F=64.26, p<0.001) compared to the 3v3 (108.24±11.26 m). Furthermore, the values of the variables Z4 (8.32±3.38 m, F=97.59), Z5 (1.84±1.53 m, F=123.64), nS (0.13±0.10 n, F=96.14) as well as Maxspeed (27.06±1.90 km/h, F=139.33), are statistically significantly higher (p<0.001) in the 10v10 game compared to the other two game formats. The average number of nAcc (0.40±0.32 n, F=9.86, p<0.001) and nDec (0.62±0.36 n, F=6.42, p<0.001) is statistically significantly higher in the 5v5 game. The results showed that the 5v5 game is significantly more metabolically demanding Pmet (2.76±0.67 W•kg−1, F=66.08, p<0.001) compared to the other two game formats.

Discussion: The data presented in this paper can be used as a basis for the construction of specific exercises based on kinematic and physiological requirements, and for planning and programming microcycles in football.
Attenuation of stretch-induced arrhythmias following chemical ablation of Purkinje fibres, in isolated rabbit hearts
Authors: Hurley Miriam , Walton Richard , Vigmond Edward J. , Haïssaguerre Michel , Bernus Olivier , White Ed
Published: April 2023

Purkinje fibres (PFs) play an important role in some ventricular arrhythmias and acute ventricular stretch can evoke mechanically-induced arrhythmias. We tested whether Purkinje fibres, play a role in these arrhythmias. Pseudo-ECGs were recorded in isolated, Langendorff-perfused, rabbit hearts in which the left ventricular endocardial surface was also irrigated with Tyrode, via an indwelling catheter placed in the left ventricular lumen. The number and period of ectopic activations was measured during left ventricular lumen inflation via an indwelling fluid-filled balloon (500 μL added over 2 s and maintained for 15 s in total). Mechanically-induced arrhythmias occurred in 70% of balloon inflations: they were maximal in the first 5 s and ceased within 15 s. Brief, (10 s) irrigation of the left ventricular lumen with Lugol solution (IK/I2), via the indwelling catheter, reduced inflation-induced ectopics by 98% (p < 0.05). Ablation of endocardial PFs by Lugol was confirmed by Triphenyltetrazolium Chloride staining. Optical mapping revealed the left ventricular epicardial activation patterns of ectopics could have PF-mediated and focal sources. In silico modelling predicted ectopic sources originating in the endocardial region propagate to and through the Purkinje fibres network. Acute distention-induced ectopics are multi-focal, their attenuation by Lugol, their activation patterns and in silico modelling indicate a participation of Purkinje fibres in these arrhythmias.
Roles of DNA damage in renal tubular epithelial cells injury
Authors: Wang Peipei , Ouyang Jing , Jia Zhanjun , Zhang Aihua , Yang Yunwen
Published: April 2023

The prevalence of renal diseases including acute kidney injury (AKI) and chronic kidney disease (CKD) is increasing worldwide. However, the pathogenesis of most renal diseases is still unclear and effective treatments are still lacking. DNA damage and the related DNA damage response (DDR) have been confirmed as common pathogenesis of acute kidney injury and chronic kidney disease. Reactive oxygen species (ROS) induced DNA damage is one of the most common types of DNA damage involved in the pathogenesis of acute kidney injury and chronic kidney disease. In recent years, several developments have been made in the field of DNA damage. Herein, we review the roles and developments of DNA damage and DNA damage response in renal tubular epithelial cell injury in acute kidney injury and chronic kidney disease. In this review, we conclude that focusing on DNA damage and DNA damage response may provide valuable diagnostic biomarkers and treatment strategies for renal diseases including acute kidney injury and chronic kidney disease.
Distinct metabolite classes in root exudates are indicative for field- or hydroponically-grown cover crops
Authors: Heuermann Diana , Döll Stefanie , Schweneker Dörte , Feuerstein Ulf , Gentsch Norman , von Wirén Nicolaus
Published: April 2023

Introduction

Plants release a large variety of metabolites via their roots to shape physico-chemical soil properties and biological processes in the rhizosphere. While hydroponic growth conditions facilitate accessibility of the root system and recovery of root exudates, the natural soil environment can alter root metabolism and exudate secretion, raising the question to what extent the quantity and composition of root exudates released in hydroponic growth systems reflect those recovered from soil-grown roots.

Methods

Using a root washing method, we sampled root exudates from four field-grown cover crop species with wide taxonomic distance, namely white mustard, lacy phacelia, bristle oat, and Egyptian clover. A set of primary metabolites and secondary metabolites were analysed in a targeted and untargeted LC-MS-based approach, respectively, for comparison with exudates obtained from hydroponically cultured plants.

Results and discussion

We found that hydroponically cultivated plants released a larger amount of total carbon, but that the recovery of total carbon was not indicative for the diversity of metabolites in root exudates. In the field, root exudates from phacelia and clover contained 2.4 to 3.8 times more secondary metabolites, whereas carbon exudation in hydroponics was 5- to 4-fold higher. The composition of the set of metabolites identified using the untargeted approach was much more distinct among all species and growth conditions than that of quantified primary metabolites. Among secondary metabolite classes, the presence of lipids and lipid-like molecules was highly indicative for field samples, while the release of a large amount of phenylpropanoids, organoheterocyclic compounds or benzenoids was characteristic for clover, mustard or oat, respectively, irrespective of the cultivation condition. However, at the compound level the bulk of released metabolites was specific for cultivation conditions in every species, which implies that hydroponically sampled root exudates poorly reflect the metabolic complexity of root exudates recovered from field-grown plants.
In situ detection and mass spectrometry imaging of protein-related metabolites in Bombyx batryticatus before and after frying with wheat bran
Authors: Liu Pai , Wang Jie-Min , Guo Hao-Chuan , Zhao Meng-Wei , Song Yong-Xing , Guo Hui , Duan Xu-Hong , Yan Yu-Ping , Zheng Yu-Guang
Published: April 2023

Bombyx batryticatus is derived from the dried larva of Bombyx mori Linnaeus infected by Beauveria bassiana (Bals.) Vuillant. Raw Bombyx batryticatus should be stir-fried before oral administration due to its irritation to the gastrointestinal tract. Nevertheless, it is still an arduous task to uncover the intrinsic mechanism of Bombyx batryticatus processing. In this study, we collected two types of Bombyx batryticatus, one being stir-fried and the other serving as a control. Then, an informative approach, which integrated matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) with chemometrics analysis, was established to screen processing-associated markers and reveal in situ spatial distribution patterns of protein-related metabolites. After optimization of experimental conditions, 21 ions were initially detected from Bombyx batryticatus, including amino acids and peptides. In addition, 15 differential markers were screened by orthogonal projection to potential structure discriminant analysis (OPLS-DA), which were localized and visualized in the transverse section of Bombyx batryticatus by MSI. Eventually, it can be demonstrated that the stir-frying process reduces toxicity while potentially boosting specific biological activities of Bombyx batryticatus. In summary, the established strategy could not only clarify the chemical transformation of protein-related metabolites from Bombyx batryticatus before and after frying with wheat bran, but also reveal the significance of Chinese medicine processing technology.
Comparative analysis of seed and seedling irradiation with gamma rays and carbon ions for mutation induction in Arabidopsis
Authors: Hase Yoshihiro , Satoh Katsuya , Kitamura Satoshi
Published: April 2023

The molecular nature of mutations induced by ionizing radiation and chemical mutagens in plants is becoming clearer owing to the availability of high-throughput DNA sequencing technology. However, few studies have compared the induced mutations between different radiation qualities and between different irradiated materials with the same analysis method. To compare mutation induction between dry-seeds and seedlings irradiated with carbon ions and gamma rays in Arabidopsis, in this study we detected the mutations induced by seedling irradiation with gamma rays and analyzed the data together with data previously obtained for the other irradiation treatments. Mutation frequency at the equivalent dose for survival reduction was higher with gamma rays than with carbon ions, and was higher with dry-seed irradiation than with seedling irradiation. Carbon ions induced a higher frequency of deletions (2−99 bp) than gamma rays in the case of dry-seed irradiation, but this difference was less evident in the case of seedling irradiation. This result supported the inference that dry-seed irradiation under a lower water content more clearly reflects the difference in radiation quality. However, the ratio of rearrangements (inversions, translocations, and deletions larger than 100 bp), which are considered to be derived from the rejoining of two distantly located DNA breaks, was significantly higher with carbon ions than gamma rays irrespective of the irradiated material. This finding suggested that high-linear energy transfer radiation induced closely located DNA damage, irrespective of the water content of the material, that could lead to the generation of rearrangements. Taken together, the results provide an overall picture of radiation-induced mutation in Arabidopsis and will be useful for selection of a suitable radiation treatment for mutagenesis.
The influences of mindfulness on high-stakes mathematics test achievement of middle school students
Authors: Zuo Haode , Wang Lidong
Published: April 2023

Research has shown that mindfulness can reduce students' negative emotions associated with high-stakes tests and thereby improve test performance. This study explored the association between mindfulness-based intervention (MBI) and high-risk math test scores of middle school students, which is noticeably slim in the domain of mathematics education, through a mediating process involving math-specific test anxiety and math self-efficacy. Using data from a sample of 45 students, age 12–13, we found empirical support for a significant positive correlation between mindfulness and middle school students' math achievement. Participants listened to a mindfulness audio every other week before a mathematics test. Weekly mathematics test scores, student group discussion data, and in-depth interview data were analyzed to explore how mindfulness affected students' mathematics test performance, which showed a statistically significant improvement after mindfulness compared to mathematics achievement without the intervention. Our results indicate that mindfulness can relieve mathematics anxiety symptoms, including physiological manifestations, test-unrelated thinking and worries, and problem-solving obstacles caused by mathematics anxiety. Also, mindfulness, especially its non-judgmental attitude, positively affects students' mathematical self-efficacy. The current research provides evidence of the mindfulness intervention's efficacy for improving middle school mathematics test performance but also identifies the complexities of implementing it with large numbers of students.
Quality of life in the older adults: The protective role of self-efficacy in adequate coping in patients with chronic diseases
Authors: Cudris-Torres Lorena , Alpi Stefano Vinaccia , Barrios-Núñez Álvaro , Gaviria Arrieta Natali , Mejía Gutiérrez Jéssica , Alvis Barranco Libia , Rios-Carlys Gerson , Cuenca-Calderón Silvia E. , Bermúdez Valmore , Hernández-Lalinde Juan , Riveira Zuleta Carlos Arturo , Bahamón Marly Johana , Álvarez Herrera Juany Sofía
Published: April 2023

The purpose of the present study was to establish the association between self-efficacy, perception of disease, emotional regulation, and fatigue and the health-related quality of life in older adults living in the departments of Cesar and Atlántico in Colombia and who have been diagnosed with a chronic disease. The participants were 325 older adults of both sexes, with literacy and no presence of cognitive impairment in the Mini-Mental State Examination (MMSE); A non-probabilistic sampling was carried out. We used the MOS-SF-36 questionnaire, the Brief Illness Perception Questionnaire scale for measuring the perception of disease, the Stanford Patient Education Research Center’s Chronic Disease Self self-efficacy questionnaire for chronic patients, the Difficulties in Emotional Regulation Scale, and the Fatigue Severity Questionnaire as measurement instruments. The design was non-experimental cross-sectional with a correlational scope. The results indicate that self-efficacy, disease perception, emotional regulation and severity of fatigue are variables that could impact the physical function of quality of life, confirming that self-efficacy would work as a factor that decreases the probability that a participant score low on this dimension of quality of life. On the other hand, both the perception of the disease and the severity of fatigue were identified as factors that probably negatively influence quality of life.
Motivation to teach and preparedness for teaching among preservice teachers in China: The effect of conscientiousness and constructivist teaching beliefs
Authors: Huang JiaLi , Sang Guoyuan , He Wenjie
Published: April 2023

“Preparedness for teaching” refers to the degree of confidence preservice teachers have, and reflects their ability. Developing preparedness for teaching is an important part of preservice teachers’ professionalization. A substantial body of literature has documented the critical influence of the motivation to teach on preparedness; however, how this relation is impacted by mediating and moderating mechanisms remains unclear. To respond to this gap in knowledge, the present study constructed a mediated moderation model through structural equation modeling and multigroup tests using 383 questionnaires completed by preservice teachers in China. The findings indicate that the preservice teachers’ genders, entry path, and levels of certainty about their future teaching career choices all influence their preparedness for teaching. Specifically, preservice teachers who believe that they will choose a teaching career in the future have more intrinsic motivation, stronger constructivist teaching beliefs, and a higher levels of teaching preparedness. Moreover, preservice teachers’ motivations to teach can positively predict their constructivist teaching beliefs and preparedness for teaching, but their constructivist teaching beliefs alone do not have a mediating effect on the relationship between motivation to teach and preparedness for teaching. However, the findings reveal that the constructivist teaching beliefs of highly conscientiousness group can partially mediate the relationship between the motivation to teach and the preparedness for teaching. Additionally, conscientiousness moderates the influence of constructivist teaching beliefs on preparedness for teaching. The study provides meaningful insights into the within-personal traits of how and when motivation to teach affects preparedness for teaching, which may be useful for the motivation best practices for preservice teacher recruitment, training, and support to create high-quality teachers.
Group-based body psychotherapy improves appreciation of body awareness in post-treatment cancer patients: A non-randomized clinical trial
Authors: Grossert Astrid , Meffert Cornelia , Hess Viviane , Rochlitz Christoph , Pless Miklos , Hunziker Sabina , Wössmer Brigitta , Geuter Ulfried , Meinlschmidt Gunther , Schaefert Rainer
Published: April 2023

Introduction

Cancer-related impairments often co-occur with bodily disturbances. Body psychotherapy (BPT) can improve bodily wellbeing, yet evidence in cancer survivors is scarce. Hence, we aimed to evaluate whether blended group BPT alleviates bodily disturbances in post-treatment cancer patients.

Methods

We conducted a bi-center study (registered in ClinicalTrials.gov, under No. NCT03707548), applying a pre-post convergent parallel design of weekly group BPT interspersed with smartphone-based ambulatory interventions using a waiting-period comparator. We included patients with completed curatively intended treatment for malignant neoplasms, suffering from bodily disturbances. The primary outcome was body image disturbances. Secondary outcomes were experiencing and appreciating body awareness, mental wellbeing, and health-related quality of life.

Results

Forty patients (mean age 51.7 years) attended group BPT. Mixed-effect linear regression models contrasting intervention with the waiting period did not show statistically significant differences regarding the primary outcome [Pre-post difference contrasts: 1.44, 95% confidence interval (CI): −1.51 to 4.93, p = 0.339]. However, patients showed greater improvements in appreciating body awareness, measured by the “Body Mindfulness Questionnaire” (BMQ), from pre- to post-intervention as compared to the waiting period (pre-post difference contrasts: 7.31 95% CI: 4.15–10.47, Bonferroni-Holm corrected q = 0.0002).

Discussion

We found no evidence that blended group BPT was effective in improving body image disturbances in post-treatment cancer patients, but found indications for an increase in body awareness appreciation.

Clinical trial registration

ClinicalTrials.gov, identifier NCT03707548.
Late-life depression: Epidemiology, phenotype, pathogenesis and treatment before and during the COVID-19 pandemic
Authors: Zhao Yuanzhi , Wu Xiangping , Tang Min , Shi Lingli , Gong Shuang , Mei Xi , Zhao Zheng , He Jiayue , Huang Ling , Cui Wei
Published: April 2023

Late-life depression (LLD) is one of the most common mental disorders among the older adults. Population aging, social stress, and the COVID-19 pandemic have significantly affected the emotional health of older adults, resulting in a worldwide prevalence of LLD. The clinical phenotypes between LLD and adult depression differ in terms of symptoms, comorbid physical diseases, and coexisting cognitive impairments. Many pathological factors such as the imbalance of neurotransmitters, a decrease in neurotrophic factors, an increase in β-amyloid production, dysregulation of the hypothalamic-pituitary-adrenal axis, and changes in the gut microbiota, are allegedly associated with the onset of LLD. However, the exact pathogenic mechanism underlying LLD remains unclear. Traditional selective serotonin reuptake inhibitor therapy results in poor responsiveness and side effects during LLD treatment. Neuromodulation therapies and complementary and integrative therapies have been proven safe and effective for the treatment of LLD. Importantly, during the COVID-19 pandemic, modern digital health intervention technologies, including socially assistive robots and app-based interventions, have proven to be advantageous in providing personal services to patients with LLD.
Typical disease courses of patients with unipolar depressive disorder after in-patient treatments–results of a cluster analysis of the INDDEP project
Authors: Martinek Theresa , Jarczok Marc , Rottler Edit , Hartmann Armin , Zeeck Almut , Weiß Heinz , von Wietersheim Jörn
Published: April 2023

Introduction

Previously established categories for the classification of disease courses of unipolar depressive disorder (relapse, remission, recovery, recurrence) are helpful, but insufficient in describing the naturalistic disease courses over time. The intention of the present study was to identify frequent disease courses of depression by means of a cluster analysis.

Methods

For the longitudinal cluster analysis, 555 datasets of patients who participated in the INDDEP (INpatient and Day clinic treatment of DEPression) study, were used. The present study uses data of patients with at least moderate depressive symptoms (major depression) over a follow-up period of 1 year after their in-patient or day-care treatments using the LIFE (Longitudinal Interval Follow-Up Evaluation)-interview. Eight German psychosomatic hospitals participated in this naturalistic observational study.

Results

Considering only the Calinski–Harabatz index, a 2-cluster solution gives the best statistical results. In combination with other indices and clinical interpretations, the 5-cluster solution seems to be the most interesting. The cluster sizes are large enough and numerically balanced. The KML-cluster analyses revealed five well interpretable disease course clusters over the follow-up period: “sustained treatment response” (N = 202, 36.4% of the patients), “recurrence” (N = 80, 14.4%), “persisting relapse” (N = 115, 20.7%), “temporary relapse” (N = 95, 17.1%), and remission (N = 63, 11.4%).

Conclusion

The disease courses of many patients diagnosed with a unipolar depression do not match with the historically developed categories such as relapse, remission, and recovery. Given this context, the introduction of disease course trajectories seems helpful. These findings may promote the implementation of new therapy options, adapted to the disease courses.
Therapist perceptions of the implementation of a new screening procedure using the ItFits-toolkit in an iCBT routine care clinic: A mixed-methods study using the consolidated framework for implementation research
Authors: Tarp Kristine , Nielsen Søren Lange , Holmberg Trine Theresa , Dalsgaard Caroline Høier , Borkner Simone , Skaarnes Helene , Jensen Esben Kjems , Piera-Jiménez Jordi , Vis Christiaan , Mathiasen Kim
Published: April 2023

Introduction

This study investigates the implementation of a new, more automated screening procedure using the ItFits-toolkit in the online clinic, Internet Psychiatry (iPsych) (www.internetpsykiatrien.dk), delivering guided iCBT for mild to moderate anxiety and depressive disorders. The study focuses on how the therapists experienced the process.

Methods

Qualitative data were collected from semi-structured individual interviews with seven therapists from iPsych. The interviews were conducted using an interview guide with questions based on the Consolidated Framework for Implementation Research (CFIR). Quantitative data on the perceived level of normalization were collected from iPsych therapists, administrative staff, and off-site professionals in contact with the target demographic at 10-time points throughout the implementation.

Results

The therapists experienced an improvement in the intake procedure. They reported having more relevant information about the patients to be used during the assessment and the treatment; they liked the new design better; there was a better alignment of expectations between patients and therapists; the patient group was generally a better fit for treatment after implementation; and more of the assessed patients were included in the program. The quantitative data support the interview data and describe a process of normalization that increases over time.

Discussion

The ItFits-toolkit appears to have been an effective mediator of the implementation process. The therapists were aided in the process of change, resulting in an enhanced ability to target the patients who can benefit from the treatment program, less expenditure of time on the wrong population, and more satisfied therapists.
Lethal and severe violence: Characterizing Swedish female offenders with and without a severe mental disorder
Authors: Trägårdh Karin , Hildebrand Karlén Malin , Andiné Peter , Nilsson Thomas
Published: April 2023

Aim

In studies on lethal and severe violence, male offenders have historically been in focus while female offenders, in comparison, have often been excluded. In this study, we aimed to characterize female violent offenders and compared those with and without a severe mental disorder (SMD).

Method

All females charged with lethal or attempted lethal violence, who had undergone forensic psychiatric investigations (FPI) in Sweden between 2000 and 2014, constituting the two groups SMD (n = 84) and no SMD (n = 91), were included. Information from their FPI reports and court verdicts was collected regarding background and demographics, mental health, substance use, and crime characteristics.

Results

Overall, both groups were often unemployed, previously victimized within close relations, had psychiatric health issues, and more than half of them had previously attempted suicide. Specifically, the SMD group more often had psychotic disorders, had attempted homicide-suicide (at the time of the crime), and had children or friends/acquaintances as victims. The no-SMD group more often manifested patterns of anxiety, personality disorders, and substance use disorders compared to the SMD group. The no-SMD group also differed from the SMD group by more often having a previous criminal record, being charged with lethal index violence, having male adult intimate partners/ex-intimate partners as victims who had abused the offender, and both offender and victim had more often been under the influence of a substance.

Conclusion

Female offenders of lethal and severe violence had a high prevalence of previous violent victimization which should be considered in forensic assessment and treatment regardless of the offender’s SMD status. However, more focus on substance use disorders and intimate partner relations appears relevant for females without an SMD. Contrary to that, early interventions regarding psychotic processes are probably a helpful preventive measure for females with an SMD. In sum, the heterogeneity of female offenders of lethal and severe violence emphasizes the necessity of developing nuanced interventions to meet their rehabilitative needs as well as the requirements of community protection.
Unraveling astrocyte behavior in the space brain: Radiation response of primary astrocytes
Authors: Roggan Marie Denise , Kronenberg Jessica , Wollert Esther , Hoffmann Sven , Nisar Hasan , Konda Bikash , Diegeler Sebastian , Liemersdorf Christian , Hellweg Christine E.
Published: April 2023

Introduction

Exposure to space conditions during crewed long-term exploration missions can cause several health risks for astronauts. Space radiation, isolation and microgravity are major limiting factors. The role of astrocytes in cognitive disturbances by space radiation is unknown. Astrocytes' response toward low linear energy transfer (LET) X-rays and high-LET carbon (12C) and iron (56Fe) ions was compared to reveal possible effects of space-relevant high-LET radiation. Since astronauts are exposed to ionizing radiation and microgravity during space missions, the effect of simulated microgravity on DNA damage induction and repair was investigated.

Methods

Primary murine cortical astrocytes were irradiated with different doses of X-rays, 12C and 56Fe ions at the heavy ion accelerator GSI. DNA damage and repair (γH2AX, 53BP1), cell proliferation (Ki-67), astrocytes' reactivity (GFAP) and NF-κB pathway activation (p65) were analyzed by immunofluorescence microscopy. Cell cycle progression was investigated by flow cytometry of DNA content. Gene expression changes after exposure to X- rays were investigated by mRNA-sequencing. RT-qPCR for several genes of interest was performed with RNA from X-rays- and heavy-ion-irradiated astrocytes: Cdkn1a, Cdkn2a, Gfap, Tnf, Il1β, Il6, and Tgfβ1. Levels of the pro inflammatory cytokine IL-6 were determined using ELISA. DNA damage response was investigated after exposure to X-rays followed by incubation on a 2D clinostat to simulate the conditions of microgravity.

Results

Astrocytes showed distinct responses toward the three different radiation qualities. Induction of radiation-induced DNA double strand breaks (DSBs) and the respective repair was dose-, LET- and time-dependent. Simulated microgravity had no significant influence on DNA DSB repair. Proliferation and cell cycle progression was not affected by radiation qualities examined in this study. Astrocytes expressed IL-6 and GFAP with constitutive NF-κB activity independent of radiation exposure. mRNA sequencing of X-irradiated astrocytes revealed downregulation of 66 genes involved in DNA damage response and repair, mitosis, proliferation and cell cycle regulation.

Discussion

In conclusion, primary murine astrocytes are DNA repair proficient irrespective of radiation quality. Only minor gene expression changes were observed after X-ray exposure and reactivity was not induced. Co-culture of astrocytes with microglial cells, brain organoids or organotypic brain slice culture experiments might reveal whether astrocytes show a more pronounced radiation response in more complex network architectures in the presence of other neuronal cell types.
The triglyceride glucose-waist-to-height ratio outperforms obesity and other triglyceride-related parameters in detecting prediabetes in normal-weight Qatari adults: A cross-sectional study
Authors: Al Akl Neyla S. , Haoudi Elias N. , Bensmail Halima , Arredouani Abdelilah
Published: April 2023

Introduction

The triglyceride-glucose (TyG)-driven indices, incorporating obesity indices, have been proposed as reliable markers of insulin resistance and related comorbidities such as diabetes. This study evaluated the effectiveness of these indices in detecting prediabetes in normal-weight individuals from a Middle Eastern population.

Methods

Using the data of 5,996 adult Qatari participants from the Qatar Biobank cohort, we employed adjusted logistic regression to assess the ability of various obesity and triglyceride-related indices to detect prediabetes in normal-weight (18.5 ≤ BMI <25 kg/m2) adults (≥18 years).

Results

Of the normal-weight adults, 13.62% had prediabetes. TyG-waist-to-height ratio (TyG-WHTR) was significantly associated with prediabetes among normal-weight men [OR per 1-SD 2.68; 95% CI (1.67–4.32)] and women [OR per 1-SD 2.82; 95% CI (1.61–4.94)]. Compared with other indices, TyG-WHTR had the highest area under the curve (AUC) value for prediabetes in men [AUC: 0.76, 95% CI (0.70–0.81)] and women [AUC: 0.73, 95% CI (0.66–0.80)], and performed significantly higher than other indices (p < 0.05) in detecting prediabetes in men. Tyg-WHTR shared similar diagnostic values as fasting plasma glucose (FPG).

Discussion

Our findings suggest that the TyG-WHTR index could be a better indicator of prediabetes for general clinical usage in normal weight Qatari adult men than other obesity and TyG-related indices. TyG-WHTR can help identify a person’s risk for developing prediabetes in both men and women when combined with FPG results.
Food insecurity indicators of 14 OECD countries in a health economics aspect: A comparative analysis
Authors: Yılmaz Salim , Günal Ahmet Murat
Published: April 2023

Introduction

Food insecurity is a critical issue that refers to a lack of access to adequate food to support a healthy and active lifestyle. This problem has wide-reaching effects and can negatively impact health, education, and overall well-being. Addressing food insecurity requires a multifaceted approach that involves the efforts of governments, organizations, and individuals to ensure access to a balanced and nutritious diet for all.

Methods

The aim of this study is to shed light on macro-level models and evaluate food insecurity risk in international comparisons. We considered six criteria to evaluate food insecurity risk in terms of health expenditure, gross domestic product (GDP) per capita, and GDP growth rate among 14 Organisation for Economic Co-operation and Development (OECD) countries. We developed a modeling approach in three stages to compare food insecurity risk and discussed the reasons for the rankings of the countries based on the model results.

Results

According to our findings, the United States has the lowest food insecurity risk, while Colombia has the highest. The results suggest that economic factors, such as GDP per capita and GDP growth rate, play a significant role in food insecurity risk. The study highlights the importance of addressing economic disparities and promoting economic growth to reduce food insecurity.

Discussion

This study provides insights into the relationship between food insecurity and economic factors, indicating that addressing economic disparities and promoting economic growth can reduce food insecurity. Future research using similar models to link economic outcomes with important health components such as nutrition and physical activity could provide a foundation for policy development.
Weight status change from birth to childhood and the odds of high blood pressure among Chinese children
Authors: Li Cheng , Liu Ziqi , Zhao Min , Zhang Cheng , Bovet Pascal , Xi Bo
Published: April 2023

Background

It is well documented that birth weight and childhood weight are associated with the blood pressure (BP) levels in childhood. However, the impact of weight status change from birth to childhood on BP among children is less well described. We aimed to assess the association between changes in weight status from birth to childhood and high BP in childhood.

Methods and results

Data were obtained from a cross-sectional survey conducted in Jinan, China, and a total of 5,546 children aged 6–17 years were included in this study. Based on the birth weight status [high weight (> 4,000 g) vs. normal weight (2,500–4,000 g)] and childhood weight status during the survey period [high weight (overweight and obesity) vs. normal weight], children were assigned into four groups: persistently normal weight (normal birth weight and normal childhood weight), resolved high weight (high birth weight but normal childhood weight), incident high weight (normal birth weight but high childhood weight), and persistently high weight (high birth weight and high childhood weight). After adjustment for sex and age, BP in childhood was more responsive to current body mass index (BMI) than birth weight. After adjustment for the potential covariates, compared with children who had persistently normal weight from birth to childhood, those with incident high weight (odds ratio [OR] = 3.88, 95% confidence interval [CI] = 3.29–4.57) and persistently high weight (OR = 3.52, 95% CI = 2.71–4.57) were associated with the increased odds of childhood high BP. However, children who had resolved high weight did not have significantly increased odds of high BP in childhood (OR = 0.86, 95% CI = 0.59–1.25).

Conclusion

The association of BP with recent BMI was stronger than with birth weight. Children who had incident or persistently high weight from birth to childhood had increased odds of high BP in childhood, whereas the odds was not significantly increased among those with high birth weight but changed to normal weight in childhood. Our findings highlight the importance of maintaining an appropriate weight in the early lifetime for the prevention of high BP and other related diseases, especially for those with high birth weight.
Satisfaction with pediatric telehealth according to the opinions of children and adolescents during the COVID-19 pandemic: A literature review
Authors: Kodjebacheva Gergana Damianova , Culinski Taylor , Kawser Bushra , Amin Saman
Published: April 2023

Objective

To review satisfaction with telehealth among children and adolescents based on their own opinions during the COVID-19 pandemic.

Methods

In the PubMed, CINAHL, PsycINFO, and Embase databases, we searched for peer-reviewed studies in English on satisfaction with telehealth among children and adolescents (rather than parents). Both observational studies and interventions were eligible. The review was categorized as a mini review because it focused on the limited time frame of the COVID-19 pandemic. We followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. Reviewers extracted information from each study and assessed risk of bias.

Results

A total of 14 studies were eligible. Studies were conducted in Australia, Canada, Italy, Israel, Poland, South Korea, the United Kingdom, and the United States. They focused on a variety of health conditions. Two of the 14 studies were interventions. Participants expressed high satisfaction with video and telephone visits and home telemonitoring while also preferring a combination of in-person visits and telehealth services. Factors associated with higher satisfaction with telehealth included greater distance from the medical center, older age, and lower anxiety when using telehealth. In qualitative studies, preferred telehealth features among participants included: a stable Internet connection and anonymity and privacy during telehealth visits.

Conclusion

Telehealth services received favorable satisfaction ratings by children and adolescents. Randomized-controlled trials on the effectiveness of pediatric telehealth services compared to non-telehealth services may assess improvements in satisfaction and health outcomes.
Trends and future outlooks in circularity of desalination membrane materials
Authors: Liang Yuanzhe , Knauer Katrina M.
Published: April 2023

Reverse osmosis desalination is one of the most important and increasingly popular technologies to augment available water resources. Central to the technology is a thin-film-composite polyamide membrane capable of separating pure water from seawater or brine. Since its conception and initiation, the membrane industry has followed a linear life-cycle scheme. However, increasing production costs of fossil-based materials and more stringent environmental regulations drive the initiatives to adapt to a circular economy of membrane materials. In this perspective, we briefly summarize the pressing issues in the state-of-the-art membrane industry, then discuss the opportunities in future technology innovations with a focus on sustainable membrane manufacturing and recycling, and lastly provided an outlook for future membrane design and fabrication towards a circular economy.
The impact of guidance counselling on gender segregation: Major choice and persistence in higher education. An experimental study
Authors: Erdmann Melinda , Schneider Juliana , Pietrzyk Irena , Jacob Marita , Helbig Marcel
Published: April 2023

Gender segregation in higher education is considered one of the main drivers of persistent economic gender inequality. Yet, though there has been considerable research identifying and describing the underlying mechanisms that cause gendered educational choices in higher education, little is known about how gender segregation in higher education could be changed. Accordingly, this article aims to determine the potential of educational interventions during high school to foster gender desegregation in higher education. We focused on two different processes that contribute to gender segregation in majors among higher education graduates: first, the selection into specific majors and, second, the selection out of specific majors. We investigated whether an intensive counselling programme leads to more gender-atypical choices among high-school graduates and examined whether intensive counselling supports several indicators of students' persistence in gender-atypical majors. Based on data from an experimental study of a counselling programme for German high-school students (N = 625), we estimated the programme's effect with linear probability models and intention-to-treat analysis. Our results show that high-school graduates are more likely to choose a gender-atypical major if they have received intensive counselling. This applies more to men than to women. In addition, the programme improved some persistence indicators for students in gender-atypical majors. Although we found a significant programme effect only for perceived person–major fit and student satisfaction, the coefficients of all aspects of students' persistence show a trend indicating that the programme was beneficial for students in gender-atypical majors. As experimental studies can also be affected by various types of bias, we performed several robustness checks. All analyses indicated stable results. In conclusion, we suggest that intensive counselling programmes have the potential to reduce gender segregation in higher education. More students were motivated to choose a gender-atypical major, and different aspects of student persistence were supported by the programme for students in gender-atypical majors.
Regulation of viral RNA-dependent RNA polymerases by phosphorylation
Authors: Duflos Camille , Michiels Thomas
Published: April 2023

RNA viruses encode an RNA-dependent RNA polymerase (RdRp), which is essential for transcription and replication of their genome since host cells lack equivalent enzymes. RdRp residues were shown to be phosphorylated by host kinases in several human, animal or plant viruses including flaviviruses, picornaviruses, coronaviruses, influenza viruses and tymoviruses. RdRps can be phosphorylated on several residues by distinct host kinases. Phosphomimetic mutations of identified phosphorylated residues either positively or negatively regulate RNA synthesis or association of RdRps with RNA or other proteins. Interestingly, some RdRps evolved to recruit cellular kinases through direct protein-protein interaction, likely to promote or to tightly control their own phosphorylation. Given the essential nature of RdRps for RNA virus replication, a better knowledge of RdRps’ phosphorylation is expected to facilitate the design of future drugs that strongly affect polymerase activity.
An ADS-B Information-Based Collision Avoidance Methodology to UAV
Authors: Tong Liang , Gan Xusheng , Wu Yarong , Yang Nan , Lv Maolong
Published: April 2023

A collision avoidance method that is specifically tailored for UAVs (unmanned aerial vehicles) operating in converging airspace is proposed. The method is based on ADS-B messages and it aims to detect and resolve conflicts between UAVs. The proposed method involves two main steps. First, a UAV conflict-sensing scheme is developed, which utilizes ADS-B information flow path and analyzes the message format information. Second, an unscented Kalman filter is used to predict UAV trajectories based on the acquired ADS-B information. The predicted information is then used to determine potential conflict scenarios, and different deconfliction strategies are selected accordingly. These strategies include speed regulation, direction regulation, and compound deconfliction, and are mathematically validated using the velocity obstacle method. The feasibility and effectiveness of the proposed method are evaluated through simulation, and it is concluded that the method can significantly improve the conflict resolution capability of UAV flights. This research provides a valuable contribution to the field of UAV collision avoidance, and can serve as a theoretical foundation for further advancements in this area.