bluuebunny/crossref_metadata_2025 huggingface dataset:
Changes in Noninvasive Arterial Stiffness and Central Blood Pressure After Endovascular Abdominal Aneurysm Repair
Authors: Holewijn Suzanne , Vermeulen Jenske J. M. , van Helvert Majorie , van de Velde Lennart , Reijnen Michel M. P. J.
Published: April 2021

Purpose:

To evaluate the impact of elective endovascular aneurysm repair (EVAR) on the carotid-femoral pulse wave velocity (cfPWV) and central pressure waveform, through 1-year follow-up.

Materials and Methods:

A tonometric device was used to measure cfPWV and estimate the central pressure waveform in 20 patients with an infrarenal abdominal aortic aneurysm scheduled for elective EVAR. The evaluated central hemodynamic parameters included the central pressures, the augmentation index (AIx), and the subendocardial viability ratio (SEVR). AIx quantifies the contribution of reflected wave to the central systolic pressure, whereas SEVR describes the myocardial perfusion relative to the cardiac workload. Measurements were performed before EVAR, at discharge, and 6 weeks and 1 year after EVAR.

Results:

CfPWV was increased at discharge (12.4±0.4 vs 11.3±0.5 m/s at baseline; p=0.005) and remained elevated over the course of 1-year follow-up (6 weeks: cfPWV = 12.2±0.5 m/s; 1 year: cfPWV = 12.2±0.7 m/s, p<0.05). After an initial drop in systolic central pressure at discharge, all the central pressures increased thereafter up to 1 year, without significant differences compared with baseline. The same was observed for the AIx and SEVR.

Conclusion:

Endovascular aortic aneurysm repair caused an increase in pulse wave velocity compared with baseline, which remained elevated through 1 year follow-up, which may be related to an increased cardiovascular risk. However, no differences in central pressure, augmentation index, and subendocardial viability ration were observed during follow-up.
Technical Aspects of Fenestrated Arch TEVAR With Preloaded Fenestration
Authors: Prendes Carlota F. , Banafsche Ramin , Stana Jan , Binskin Robert , Konstantinou Nikolaos , Kölbel Tilo , Tsilimparis Nikolaos
Published: April 2021

Fenestrated thoracic endovascular aortic repair (f-TEVAR) has expanded the possibilities of endovascular arch repair, allowing treatment of pathologies involving the aortic arch that require sealing in Ishimaru zones 1 and 2. The growing number of implantations has increased physician experience and helped identify critical procedural points, mainly wire entanglement and device malrotation. Herein we describe a step-by-step approach to a f-TEVAR procedure with the Zenith fenestrated preloaded thoracic endograft, identifying potential pitfalls and suggesting problem-solving solutions.
Incidence of Loss of Reduction After Open Reduction Internal Fixation of Metacarpal Shaft Fractures
Authors: Allen Scott T. , Wang Olivia J. , Erickson Lauren O. , Ward Christina M.
Published: April 2021

Background

This study compared the incidence of loss of reduction (LOR) between metacarpal fractures fixed with screws alone and those fixed with plates and screws. Secondary aims included identifying patient or fracture characteristics associated with increased risk of LOR.

Methods

We retrospectively reviewed 138 metacarpal fractures in 106 patients treated with open reduction internal fixation with screws (60 fractures) or plates and screws (78 fractures) with a mean radiographic follow-up of 50 days for evidence of LOR. We compared the incidence of LOR between the screw and plate groups using a χ2 test. We performed logistic regression analysis to determine whether patient age, sex, metacarpal location (index, long, ring, small), the presence of multiple metacarpal fractures, or fracture pattern were associated with increased incidence of LOR.

Results

Loss of reduction occurred in 19 (13.8%) of 138 fractures, with no statistically significant difference between lag screw (7 of 60, 11.6%) and plate fixation (12 of 78, 15.4%). Neither fracture pattern nor the presence of multiple metacarpal fractures was associated with an increased incidence of LOR, but patients experienced a 7% increase in the risk of LOR for each additional year of age. Loss of reduction occurred most frequently in index metacarpal fractures (4 of 12, 33%), although this did not reach statistical significance.

Conclusions

We found no difference in LOR incidence between lag screw fixation and plate fixation. The overall incidence of LOR was higher in this study than previously reported and increased with increasing patient age.
Hygrothermal performance of cool roofs with reflective coating material subjected to hot, humid and dusty climate
Authors: Saber Hamed H
Published: April 2021

The measurements for the short-wave solar reflectivity of a Reflective Coating Material (RCM) with various cleaning operations that were obtained in a previous study were used in this study to conduct numerical simulations in order to assess the moisture and energy performance of cool and black roofs when they were subjected to the weather conditions of Saudi Eastern Province and Kuwait City. The results of the numerical simulations showed that black roofs always work with less moisture than cool roofs. Because the highest relative humidity in the different components of the black and cool roofs was well below 80%, there was no risk of condensation and mold growth in these roofs. For both weather conditions of Saudi Eastern Province and Kuwait City, the results showed that installing cool roofs have resulted in increasing the heating energy loads in relation to black roofs. Conversely, the results showed that the decrease in the cooling energy loads due to installing cool roofs were typically much greater than the increase in the heating energy loads. As such, cool roofs have resulted in net energy savings in relation to black roofs. Replacing black roof by cool roof in Saudi climate, the results showed that the annual energy savings in the total energy load was 25% and 34% as a result of installing cool roof with RCM at no cleaning and weekly homemade cleaning, respectively. Additionally, replacing black roof by cool roof in Kuwaiti climate, the results showed that the annual energy savings in the total energy load was 23% and 31% a result of installing cool roof with RCM at no cleaning and weekly homemade cleaning, respectively.
The S-STARTS Test: Validation of a Composite Test for the Assessment of Readiness to Return to Sport After Shoulder Stabilization Surgery
Authors: Juré Dimitri , Blache Yoann , Degot Matthieu , Vigne Grégory , Nové-Josserand Laurent , Godenèche Arnaud , Collotte Philippe , Franger Gabriel , Borel Florent , Rogowski Isabelle , Neyton Lionel
Published: April 2021

Background:

The time elapsed since surgery is the primary criterion for allowing athletes to return to sport after shoulder stabilization surgery using the Latarjet procedure. The objective assessment of shoulder functional status through the return-to-sport continuum demands a scoring instrument that includes psychological and physical dimensions. This study aimed to statistically validate the Shoulder-SanTy Athletic Return To Sport (S-STARTS) score in patients who have undergone primary shoulder stabilization surgery.

Hypothesis:

The S-STARTS score fulfils the criteria for statistical validation for assessing return-to-sport readiness after shoulder stabilization surgery.

Study Design:

Diagnostic study.

Level of Evidence:

Level 4.

Methods:

Fifty patients and 50 controls completed the Shoulder Instability-Return to Sport after Injury questionnaire and performed 4 physical performance tests, from which 8 outcome measures were extracted to provide a composite score, named S-STARTS, according to a scoring procedure. The statistical validation of the S-STARTS score was based on construct validity, discriminant validity, sensitivity to change, internal consistency, reliability, agreement, and feasibility.

Results:

The 8 components of the S-STARTS score provided additional information (0.01 ≤ | r| ≤ 0.59). The S-STARTS score exhibited good reliability (intraclass coefficient of correlation [3,k] = 0.74), no ceiling or floor effects, and high discrimination and sensitivity to change. The S-STARTS score was significantly lower in patients than in controls (13.5 ± 3.8 points vs 16.1 ± 2.7 points, respectively; P < 0.001). A significant increase was reported between 4.5 and 6.5 months postoperatively (12.8 ± 2.3 points vs 17.2 ± 2.4 points, respectively; P < 0.001).

Conclusions:

The S-STARTS score meets statistical validation criteria for the assessment of shoulder functional status after shoulder stabilization surgery using the Latarjet procedure.

Clinical Relevance:

Using an S-STARTS score–based assessment to monitor an athlete’s progression through the return-to-sport continuum may help clinicians and strength and conditioning coaches in return-to-sport decision-making.
FGD5-AS1 Inhibits Osteoarthritis Development by Modulating miR-302d-3p/TGFBR2 Axis
Authors: Yang Yue , Sun Zhibo , Liu Feng , Bai Yuanzhang , Wu Fei
Published: April 2021

Objective

Osteoarthritis (OA) is a common joint disorder, accompanied by extracellular matrix (ECM) degradation. Reportedly, long noncoding RNAs (lncRNAs) are involved in OA pathogenesis. However, the role of lncRNA FYVE, RhoGEF, and PH domain containing 5 antisense RNA 1 (FGD5-AS1) in OA development is still not fully clarified. This study was aimed to clarify the role of FGD5-AS1 in OA.

Methods

FGD5-AS1 and miR-302d-3p expression levels were determined in cartilage tissues and chondrocytes by quantitative real-time polymerase chain reaction (qRT-PCR). Chondrocytes (C20/A4 cells) were stimulated with interleukin 1β (IL-1β) to mimic the inflammatory environment of OA. Cell viability was detected by cell counting kit-8 and 5-ethynyl-2′-deoxyuridine assays. Cell apoptosis was measured by the caspase-3 activity assay and flow cytometry. Transforming growth factor beta receptors II (TGFBR2), matrix metalloproteinase 13 (MMP-13), and ADAM metallopeptidase with thrombospondin type 1 motif 5 expression levels were examined by qRT-PCR or Western blot. The regulatory relationships among FGD5-AS1, miR-302d-3p, and TGFBR2 were predicted by the StarBase v2.0, miRanda, miRDB, and TargetScan databases, and confirmed by dual-luciferase reporter assay and RNA immunoprecipitation assay.

Results

FGD5-AS1 and TGFBR2 expression levels were downregulated while miR-302d-3p expression was increased in cartilage tissues of OA patients. Knocking down FGD5-AS1 inhibited the viability of C20/A4 cells but induced apoptosis and ECM degradation, while FGD5-AS1 overexpression exerted opposite effects. MiR-302d-3p was identified as a target of FGD5-AS1, and TGFBR2 was identified as a target of miR-302d-3p. FGD5-AS1 positively regulated TGFBR2 expression by repressing miR-302d-3p expression, and miR-302d-3p inhibition or TGFBR2 restoration reversed the changes of cell viability, apoptosis, and ECM degradation induced by FGD5-AS1 knockdown.

Conclusion

FGD5-AS1 can probably inhibit OA progression by regulating miR-302d-3p/TGFBR2 axis.
Vasoactive intestinal peptide producing pheochromocytoma and intracardiac thrombosis
Authors: Hermel Melody , Jones Daniel , Olson Cheryl , Sherman Mark , Srivastava Ajay
Published: April 2021

A case of pheochromocytoma producing vasoactive intestinal peptide (VIP) and left ventricular thrombus in the absence of cardiomyopathy or wall motion abnormalities on echocardiogram is presented along with a review of the relevant literature. A 30-year-old female of Afghani descent with past medical history of panic attacks presented with fever, cough, sore throat, vomiting, and was found to have an 11 cm adrenal mass consistent with primary adrenocortical adenoma versus carcinoma. Her tumor elicited catechols and vasoactive intestinal peptide. Her hospitalization was complicated by left ventricular thrombosis leading to an embolic injury to her right kidney, respiratory failure, need for transient dialysis and urinary tract infections. She developed a profuse secretory diarrhea and decision was made to treat with empiric octreotide infusion and imodium with improvement in symptoms. She underwent coil and particle embolization followed by resection. Followup PET gallium scan showed no evidence of residual disease or metastasis. VIP producing pheochromocytoma associated with intracardiac thrombosis is rare. Outcomes depend on prompt diagnosis of the pheochromocytoma and multidisciplinary approach to management.
A case of metachronous bilateral secretory carcinoma
Authors: Burlile Jessica , Collins Andrea , Garcia Joaquin , Gendron Kristin , Cunningham Danielle , Foote Robert
Published: April 2021

Secretory carcinoma (SC) was first recognized as a distinct salivary malignancy in 2010. In the nine years since its recognition, there have been multiple reports of SC of the major and minor salivary glands, as well one case of tongue base involvement. Here we present the first reported case of bilateral SC. The first tumor, diagnosed before the recognition of SC, was classified as mucoepidermoid carcinoma. After the contralateral parotid tumor was diagnosed as SC in 2016, the two histologies were compared, and the mucoepidermoid carcinoma was reclassified as SC. In this report, we describe our patient’s clinical course and review the SC literature, with a focus on pathologic diagnosis and clinical prognosis.
A case study: How did IoT start-up Distronix change its business model to sustain growth in the pay-per-use economy
Authors: Chakrabarti Dipankar , Kumar Rohit , Sarkar Soumya , Mukherjee Arindam
Published: April 2021

Industrial Internet of Things emerged as one of the major technologies enabling Industry 4.0 for industries. Multiple start-ups started working in the Industrial Internet of Things field to support this new industrial revolution. Distronix, one such Industrial Internet of Things start-up of India, started operations in 2014, when companies were not even aware of Industrial Internet of Things. Distronix started executing fixed-fee projects for implementation of Industrial Internet of Things. They also started manufacturing sensors to support large customers end-to-end in their Industry 4.0 journey. With the advent of public cloud, companies started demanding pay-per-use model for the solution Distronix provided. This posed a major challenge to Distronix as they had developed technology skills focusing fixed-fee customized project delivery for their clients. The situation demanded that they change their business model from individual project delivery to creation of product sand-box with pre-registered sensors and pre-defined visualization layer to support use cases for Industrial Internet of Things implementation in multiple industry sectors. It forced Rohit Sarkar, the 26 years old entrepreneur and owner of Distronix, to upgrade capabilities of his employees and transform the business model to support pay-per-use economy popularized by public cloud providers. The case discusses the challenges Rohit faced to revamp their business model in such an emerging technology field, like, to develop new skills of the technical people to support such novel initiative, reorienting sales people towards pay as use model, developing new concept of plug and play modular product, devising innovative pricing, better alliance strategy and finding out a super early adopter.
Histoplasmosis overlapping with HIV and tuberculosis in sub-Saharan Africa: challenges and research priorities
Authors: Kuate Marius Paulin Ngouanom , Ekeng Bassey Ewa , Kwizera Richard , Mandengue Christine , Bongomin Felix
Published: April 2021

Histoplasmosis, tuberculosis and HIV are all highly prevalent in sub-Saharan Africa (SSA). Co-occurrence of two or more of these infections has been reported in several populations of patients, especially those with advanced HIV infection where these opportunistic infections contribute to a significant morbidity and mortality. With a high burden of pulmonary tuberculosis (PTB) secondary to HIV in SSA, histoplasmosis is commonly misdiagnosed as smear-negative PTB in HIV patients due to similar clinical and radiological presentations. This is also partly the result of the lack of trained clinical and laboratory personnel to make a definite diagnosis of histoplasmosis. There is a low index of clinical suspicion for histoplasmosis, and cases are mostly discovered accidently and documented through case reports and case series. Similarly, the high cost and lack of fungal diagnostics in most SSA countries makes it difficult to make a diagnosis. There is a need to build local capacity for mycology so that patients are managed to improve on the index of clinical suspicion and diagnostic capabilities. Moreover, simple accurate point-of-care diagnostic tests and first-line antifungal treatment for histoplasmosis are not available in many SSA countries. This review describes the existence of co-infections of histoplasmosis, tuberculosis and HIV in SSA, highlighting the challenges and research priorities.
Can the Popular Disembody Populism? Students and the Re-appropriation of the Nationalist Floating Signifier in Contemporary Indian Politics
Authors: Martelli Jean-Thomas
Published: April 2021

This ethnographic account chronicles the journey of one of the largest anti-government protests since India’s independence. It examines the pivotal role of students—initially activists and then first-time participants—in crystallizing challenges to the ruling dispensation, not only by opposing it directly, but through subverting its way of claiming representation. More specifically, it is the strategic reuse of the pervasive anti-institutional and anti-elite discourse at the top—while replacing its majoritarianism with inclusiveness—that enabled protesters to disembody the populist modality of the current Indian Prime Minister. Protesters’ short-lived success was achieved through an enactment of the popular, embodied in a diffused fashion by faceless, peaceful and feminized protesting masses. The popular successfully appropriated the claim to be the people through invoking a ‘derivative’ nationalist repertoire in part shared by the government, emptying its anti-minorities subtext through appropriating floating signifiers of patriotic belonging such as the Indian constitution, the flag and the anthem. By engaging on how relatively small communities of politicized students used the campus ecology and its neighbouring spaces as territorial and ideational nodal points for the mobilization of less politicized cohorts, the article underlines their significance in the political articulation of dissent in contemporary Indian democracy.
The Politics of Knowledge in Development: An Analytical Frame
Authors: Banerjee Madhulika
Published: April 2021

The parameters of modern knowledge systems are clearly showing fault lines—that if there is a continuation of the technological systems at the heart of development, neglecting the twin issues of ecology and equity—there is a serious threat to human existence. This article seeks to answer a specific question: in the context of the twenty-first century search of offering alternatives to the hegemonic development paradigm, what kind of knowledges of production in society could possibly be best developed at this point in history? It argues that the answer lies in ‘already existing knowledge systems ( AEKS)’, accompanied by critical thinking on production, distribution and consumption systems. Locating the production of knowledge in five spaces—historical context, policy formulation, political economic structures, forms of collective action and articulation of contested epistemologies—it argues that when AEKS are understood both in form and transformation in these spaces, that the possibilities they offer for substantial alternatives can be explored.
How Many Patients Achieve an Acceptable Symptom State After Hip Arthroscopy for Femoroacetabular Impingement Syndrome? A Cross-sectional Study Including PASS Cutoff Values for the HAGOS and iHOT-33
Authors: Ishøi Lasse , Thorborg Kristian , Ørum Marie G. , Kemp Joanne L. , Reiman Michael P. , Hölmich Per
Published: April 2021

Background:

Hip arthroscopy is a viable treatment for femoroacetabular impingement syndrome (FAIS). Clinically relevant improvements in hip function and pain after surgery are often reported, but it is less clear how many patients achieve an acceptable symptom state (Patient Acceptable Symptom State [PASS]).

Purpose:

To investigate the proportion of patients who achieved a PASS 12 to 24 months after hip arthroscopy and to determine the cutoff scores of the 2 recommended and valid patient-reported outcome measures (the subscales of the Copenhagen Hip and Groin Outcome Score [HAGOS] and the International Hip Outcome Tool—33 [iHOT-33]) for which patients are most likely to achieve PASS.

Study Design:

Cohort study; Level of evidence, 3.

Methods:

Eligible study patients were identified in the Danish Hip Arthroscopy Registry. An electronic questionnaire was used to collect data on PASS, HAGOS, and iHOT-33 12 to 24 months after surgery. PASS was measured using an anchor question. Receiver operating characteristic curve analyses were applied to identify the PASS cutoff values of HAGOS and iHOT-33 scores.

Results:

A total of 137 individuals (mean age at surgery, 35.4 ± 9.4 years) were included in the study at a mean follow-up of 18.5 ± 3.2 months after surgery. At follow-up, 64 individuals (46.7%; 95% CI, 38.6-55.1) reported PASS. Higher HAGOS and iHOT-33 values were observed for participants who reported PASS compared with those who did not report PASS (Cohen d ≥ 1.06; P < .001). Cutoff scores for HAGOS subscales (42.5-82.5) and iHOT-33 (67.00) showed excellent to outstanding discriminative ability in predicting PASS (area under the curve, 0.82-0.92).

Conclusion:

In total, 46% of individuals having hip arthroscopy for FAIS achieved PASS at 12 to 24 months of follow-up. Patients who achieved PASS had statistically significant and substantially better self-reported hip function compared with those who did not achieve PASS. Cutoff values at HAGOS subscales and iHOT-33 showed excellent to outstanding discriminative ability in predicting patients with PASS.
A Taxonomic Review of Patient Complaints in Adult Hospital Medicine
Authors: Elias Richard M , Fischer Karen M , Siddiqui Mustaqeem A , Coons Trevor , Meyerhofer Cindy A , Pretzman Holly J , Greig Hope E , Stevens Sheila K , Burton M Caroline
Published: April 2021

Previous studies show that patient complaints can identify gaps in quality of care, but it is difficult to identify trends without categorization. We conducted a review of complaints relating to admissions on hospital internal medicine (HIM) services over a 26-month period. Data were collected on person characteristics and key features of the complaint. The complaints were also categorized into a previously published taxonomy. Seventy-six unsolicited complaints were identified, (3.5 per 1000 hospital admissions). Complaints were more likely on resident services. The mean duration between encounter and complaint was 18 days, and it took an average of 12 days to resolve the complaint. Most patients (59%) had a complaint in the Relationship domain. Thirty-nine percent of complaints mentioned a specific clinician. When a clinician was mentioned, complaints regarding communication and humaneness predominated (68%). The results indicate that the efforts to reduce patient complaints in HIM should focus on the Relationships domain.
Deconstructing Merit: Dalit Presence in Higher Education
Authors: Singh Kanika
Published: April 2021

Higher education is exclusionary in principle but it must not perpetuate injustice as it is antithetical to the spirit of academia. This article aims to elaborate on the condition of Indian higher education specifically in response to the question of accessibility. The current systems designed to screen students indirectly favour individuals with inherent privileges. These privileges are converted into socially sanctioned claim on opportunities through the merit discourse. What does this entail for Dalits whose academic performance and potential is questioned based on flawed systems and methods?

The primary objective of this article is to understand the complexities in the current usage of merit in Indian admission policies in higher education. The article presents multiple interpretations of merit as understood by the respondents during the primary data collection from a public university. A detailed discussion on the themes culled out from the data collection will highlight the larger problem of fetishizing merit without understanding its deeply problematic structure.

The article also investigates the invisibility of caste discrimination and hypervisibility of caste otherwise in university space against the meritocratic principle that is considered to be a better alternative to caste-based quotas with respect to admission policies in higher education institutions.
Greening extractivism: Environmental discourses and resource governance in the ‘Lithium Triangle’
Authors: Voskoboynik Daniel Macmillen , Andreucci Diego
Published: April 2021

The lithium extractive industry is expanding, as technological and economic shifts associated with climate change mitigation goals drive global demand for lithium-ion batteries. This article explores the case of the ‘Lithium Triangle’, a region of Latin America (spanning Bolivia, Chile and Argentina) that contains the world’s largest reserves, and where environmental conflicts associated with lithium mining have proliferated. Emphasising the centrality of discourse in resource governance, we analyse the discursive strategies employed by institutional actors seeking to promote and render acceptable lithium extraction in the region. We argue that such strategies reproduce imaginaries of prosperity and modernisation long attached to oil and mineral wealth, while at the same time introducing a novel association of mining with high-tech industries, ‘green jobs’ and ‘climate-friendly’ extraction, seeking to obscure the social and ecological costs of lithium production. This inaugurates an era of ‘green extractivism’, whereby intensive resource exploitation is framed not only as compatible with climate change, but indeed as necessary to its mitigation. Our findings contribute to ongoing conversations regarding post-fossil fuel ‘transitions’, by highlighting the contradictory character of mitigation strategies that rely on mineral-intensive development.
OTULIN is a new target of EA treatment in the alleviation of brain injury and glial cell activation via suppression of the NF-κB signalling pathway in acute ischaemic stroke rats
Authors: Xu Hongbei , Wang You , Luo Yong
Published: April 2021

Objective

Ovarian tumour domain deubiquitinase with linear linkage specificity (OTULIN) is a potent negative regulator of the nuclear factor-κB (NF-κB) signalling pathway, and it plays a strong neuroprotective role following acute ischemic stroke. Electroacupuncture (EA) is an effective adjuvant treatment for reducing brain injury and neuroinflammation via the inhibition of NF-κB p65 nuclear translocation, but the underlying mechanism is not clear. The present study investigated whether OTULIN was necessary for EA to mitigate brain injury and glial cell activation in a transient middle cerebral artery occlusion (tMCAO) model in rats.

Methods

An acute ischaemic stroke model was established via tMCAO surgery in Sprague–Dawley (SD) rats. EA was performed once daily at “Baihui (GV 20)”, “Hegu (LI 4)”, and “Taichong (LR 3)” acupoints. The effect of EA on the spatiotemporal expression of OTULIN in the ischaemic penumbra of the cerebral cortex was detected within 7 days after reperfusion. The effects of OTULIN gene silencing on EA neurological deficits, cerebral infarct volume, neuronal damage, the activation of microglia and astrocytes, the contents of tumour necrosis factor alpha (TNF-α), interleukin-1 beta (IL-1β) and interleukin-6 (IL-6), and the expression of p-IκBa, IκBa and nucleus/cytoplasm NF-κB p65 protein were assessed.

Results

EA treatment increased endogenous OTULIN expression, which peaked at 48 h. Enhanced OTULIN was primarily located in neurons, but a small amount of OTULIN was detected in microglia. OTULIN silencing obviously reversed EA neuroprotection, which was demonstrated by worsened neurobehavioural performance, cerebral infarct volume and neuronal injury. The inhibitory effect of EA on the NF-κB pathway was also attenuated by enhanced IκBα phosphorylation and NF-κB p65 nuclear translocation. EA partially inhibited the transformation of microglia and astrocytes from resting states to activated states and reduced the secretion of TNF-α, IL-1β and IL-6. However, these preventive effects were reversed after the silencing of OTULIN expression.

Conclusions

OTULIN provides a new potential therapeutic target for EA to alleviate acute ischaemic stroke-induced brain injury and the activation of glial cells, which are related to suppression of the NF-κB signalling pathway.
The Band-Gap Studies of Short-Period CdO/MgO Superlattices
Authors: Przeździecka Ewa , Strąk P. , Wierzbicka A. , Adhikari A. , Lysak A. , Sybilski P. , Sajkowski J. M. , Seweryn A. , Kozanecki A.
Published: April 2021

Trends in the behavior of band gaps in short-period superlattices (SLs) composed of CdO and MgO layers were analyzed experimentally and theoretically for several thicknesses of CdO sublayers. The optical properties of the SLs were investigated by means of transmittance measurements at room temperature in the wavelength range 200–700 nm. The direct band gap of {CdO/MgO} SLs were tuned from 2.6 to 6 eV by varying the thickness of CdO from 1 to 12 monolayers while maintaining the same MgO layer thickness of 4 monolayers. Obtained values of direct and indirect band gaps are higher than those theoretically calculated by an ab initio method, but follow the same trend. X-ray measurements confirmed the presence of a rock salt structure in the SLs. Two oriented structures (111 and 100) grown on c- and r-oriented sapphire substrates were obtained. The measured lattice parameters increase with CdO layer thickness, and the experimental data are in agreement with the calculated results. This new kind of SL structure may be suitable for use in visible, UV and deep UV optoelectronics, especially because the energy gap can be precisely controlled over a wide range by modulating the sublayer thickness in the superlattices.
Retrospective non-target analysis to support regulatory water monitoring: from masses of interest to recommendations via in silico workflows
Authors: Lai Adelene , Singh Randolph R. , Kovalova Lubomira , Jaeggi Oliver , Kondić Todor , Schymanski Emma L.
Published: April 2021

Background

Applying non-target analysis (NTA) in regulatory environmental monitoring remains challenging—instead of having exploratory questions, regulators usually already have specific questions related to environmental protection aims. Additionally, data analysis can seem overwhelming because of the large data volumes and many steps required. This work aimed to establish an open in silico workflow to identify environmental chemical unknowns via retrospective NTA within the scope of a pre-existing Swiss environmental monitoring campaign focusing on industrial chemicals. The research question addressed immediate regulatory priorities: identify pollutants with industrial point sources occurring at the highest intensities over two time points. Samples from 22 wastewater treatment plants obtained in 2018 and measured using liquid chromatography–high resolution mass spectrometry were retrospectively analysed by (i) performing peak-picking to identify masses of interest; (ii) prescreening and quality-controlling spectra, and (iii) tentatively identifying priority “known unknown” pollutants by leveraging environmentally relevant chemical information provided by Swiss, Swedish, EU-wide, and American regulators. This regulator-supplied information was incorporated into MetFrag, an in silico identification tool replete with “post-relaunch” features used here. This study’s unique regulatory context posed challenges in data quality and volume that were directly addressed with the prescreening, quality control, and identification workflow developed.

Results

One confirmed and 21 tentative identifications were achieved, suggesting the presence of compounds as diverse as manufacturing reagents, adhesives, pesticides, and pharmaceuticals in the samples. More importantly, an in-depth interpretation of the results in the context of environmental regulation and actionable next steps are discussed. The prescreening and quality control workflow is openly accessible within the R package Shinyscreen, and adaptable to any (retrospective) analysis requiring automated quality control of mass spectra and non-target identification, with potential applications in environmental and metabolomics analyses.

Conclusions

NTA in regulatory monitoring is critical for environmental protection, but bottlenecks in data analysis and results interpretation remain. The prescreening and quality control workflow, and interpretation work performed here are crucial steps towards scaling up NTA for environmental monitoring.
A cell-based ribozyme reporter system employing a chromosomally-integrated 5′ exonuclease gene
Authors: Aroonsri Aiyada , Kongsee Jindaporn , Gunawan Jeremy David , Aubry Daniel Abidin , Shaw Philip James
Published: April 2021

Background

Bioinformatic genome surveys indicate that self-cleaving ribonucleic acids (ribozymes) appear to be widespread among all domains of life, although the functions of only a small number have been validated by biochemical methods. Alternatively, cell-based reporter gene assays can be used to validate ribozyme function. However, reporter activity can be confounded by phenomena unrelated to ribozyme-mediated cleavage of RNA.

Results

We established a ribozyme reporter system in Escherichia coli in which a significant reduction of reporter activity is manifest when an active ribozyme sequence is fused to the reporter gene and the expression of a foreign Bacillus subtilis RNaseJ1 5′ exonuclease is induced from a chromosomally-integrated gene in the same cell.

Conclusions

The reporter system could be useful for validating ribozyme function in candidate sequences identified from bioinformatics.
Correlations among disability, anti-AQP4 antibody status and prognosis in the spinal cord involved patients with NMOSD
Authors: Hsu Jung Lung , Liao Ming-Feng , Chang Kuo-Hsuan , Cheng Mei-Yun , Ro Long-Sun
Published: April 2021

Background

Neuromyelitis optica spectrum disorder (NMOSD) is a rare neuroinflammatory disorder of the central nervous system that typically involves the optic nerve, the spinal cord and other specific brain regions. In relapse of the disease, factors associated with clinical features and lesion severity are important for clinicians to predict disease-related disability.

Methods

We retrospectively analyzed 22 female patients with NMOSD who had spinal cord lesions. Detailed clinical features, onset symptoms, motor disability, relapse episodes, serum aquaporin-4 (AQP4) and myelin oligodendrocyte glycoprotein (MOG) autoantibodies and MRI characteristics were documented to correlate their associations with the nadir and three-month Expanded Disability Status Scale (EDSS) scores. Patients with three-month EDSS scores below four (< 4) were categorized as the good outcome group, while those with scores of four or more (> 4) were categorized as the poor outcome group.

Results

In patients with NMOSD, the mean age was 44.5 ± 12.8 years, and the mean three-month EDSS score was 4.3 ± 1.9. A significantly higher all-limb muscle power score was found in the good EDSS group than in the poor EDSS group (p = 0.01). A tendency toward longer follow-up periods and lower anti-AQP4 antibody levels was found in the good outcome group. Serum anti-AQP4 antibodies were present in 86% of patients with NMOSD, and MOG autoantibodies were found in one anti-AQP4 antibody-negative patient (33.3%). In patients with NMOSD, more than 40% of spinal cord lesions were distributed at the middle cervical and upper thoracic levels.

Conclusions

Our findings suggest that EDSS scores and MRC scores at the nadir had significant associations with three-month EDSS scores. The topographic distributions of the spinal cord lesions might relate to different serum anti-AQP4 antibody status. However, further studies will be needed to corroborate this finding.
Associations between mandibular torus and types of temporomandibular disorders, and the clinical usefulness of temporary splint for checking bruxism
Authors: Lee Hee-Min , Kang Dong-Woo , Yun Pil-Young , Kim Il-hyung , Kim Young-Kyun
Published: April 2021

Background

Occlusal stress from oral parafunctional habits is one of the causes of temporomandibular disorders (TMD) and mandibular torus (MT). Although some studies have investigated the correlation between TMD and MT, understanding of the relationships between types of TMD and MT is insufficient. Therefore, we conducted this study to investigate the associations between presence of MT and TMD types.

Methods

This study included 77 patients diagnosed with TMD who first visited our clinic for TMD between March 2019 and July 2020. Among them, 30 (38.9%) had MT, and 54 (70.1%) had oral parafunction. Parafunctional activity during sleep was confirmed using a temporary splint for checking bruxism (TSCB).

Results

The relationship between prevalence of MT and oral parafunction in TMD patients was not statistically significant (P = 0.131), but the odds ratio was relatively high at 2.267. An analysis of TMD type revealed that Type I, which is classified as myalgia of the masticatory muscles, and MT had a significant association (P = 0.011). We fabricated a TSCB for 27 patients to wear during sleep and confirmed that 23 (85.2%) had nocturnal bruxism. The TSCB results and presence of MT showed a significant relationship (P = 0.047).

Conclusion

Through the results of this study, clinicians may consider the hyperactivity of masticatory muscles in the presence of MT when treating TMD patients. In addition, TSCB has a great diagnostic value as it can be easily manufactured and be useful for discovering pre-existing oral parafunctions that patients are not aware of.
What is the performance in public hospitals? A longitudinal analysis of performance plans through topic modeling
Authors: Noto Guido , Lo Verso Andrea Carlo , Barresi Gustavo
Published: April 2021

Background

Both the concept of performance and the role of hospitals in health systems evolved significantly in the last decades. Today, the performance in health could be defined as the ability to create ‘population value,’ and the hospitals’ role is to support this aim by providing acute care and by integrating and coordinating their activity with other settings of care. This research aims to assess how and with what degree the management of public hospitals have embraced in practice the updated concept of performance and their new role.

Result

The paper analyses 181 performance plans of 48 Italian autonomous public hospitals over a nine-year period through the topic modeling algorithm called Latent Dirichlet Allocation (LDA). This is a method that allows for analysing large textual corpora that generates a representation of the latent topics discussed therein. The concept of performance in public hospitals was framed into 15 topics resulting from the analysis of the hospitals’ performance plans. The prevalence of each topic was analysed through the period considered so as to understand the evolution of performance-related practices over the last decade.

Conclusion

In recent years, the concept of performance in hospitals evolved toward the adoption of an outcome-based and population-based perspective. Additional effort should be devoted toward improved collaboration and integration of care with other settings.
Towards open, reliable, and transparent ecology and evolutionary biology
Authors: O’Dea Rose E. , Parker Timothy H. , Chee Yung En , Culina Antica , Drobniak Szymon M. , Duncan David H. , Fidler Fiona , Gould Elliot , Ihle Malika , Kelly Clint D. , Lagisz Malgorzata , Roche Dominique G. , Sánchez-Tójar Alfredo , Wilkinson David P. , Wintle Bonnie C. , Nakagawa Shinichi
Published: April 2021

Unreliable research programmes waste funds, time, and even the lives of the organisms we seek to help and understand. Reducing this waste and increasing the value of scientific evidence require changing the actions of both individual researchers and the institutions they depend on for employment and promotion. While ecologists and evolutionary biologists have somewhat improved research transparency over the past decade (e.g. more data sharing), major obstacles remain. In this commentary, we lift our gaze to the horizon to imagine how researchers and institutions can clear the path towards more credible and effective research programmes.
Selenium source and level on performance, selenium retention and biochemical responses of young broiler chicks
Authors: Arnaut Pedro Righetti , da Silva Viana Gabriel , da Fonseca Lucimauro , Alves Warley Junior , Muniz Jorge Cunha Lima , Pettigrew James Eugene , e Silva Fabyano Fonseca , Rostagno Horácio Santiago , Hannas Melissa Izabel
Published: April 2021

Background

Selenium (Se) has been recognized as an essential micronutrient for nearly all forms of life. In recent decades, broiler responses to dietary Se supplemental levels and sources have received considerable attention. On environmental grounds, organic trace mineral utilization in practical broiler feeds has been defended due to its higher bioavailability. In such feeds, trace minerals are provided simultaneously in the same supplement as inorganic salts or organic chelates, a fact commonly ignored in assays conducted to validate organic trace mineral sources. The current assay aimed to investigate growth and biochemical responses, as well as Se retention of growing chicks fed diets supplemented with organic and inorganic Se levels and where the trace minerals (zinc, copper, manganese, and iron) were provided as organic chelates or inorganic salts according to Se source assessed. In so doing, a 2 × 5 factorial arrangement was used to investigate the effects of sodium selenite (SS) and selenium-yeast (SY) supplemented in feeds to provide the levels of 0, 0.08, 0.16, 0.24, and 0.32 mg Se/kg.

Results

Chicks fed selenium-yeast diets had body weight (BW), and average daily gain (ADG) maximized at 0.133 and 0.130 mg Se/kg, respectively. Both Se sources linearly increased (P < 0.05) the glutathione peroxidase (GSH-Px) activity in chick blood but higher values were observed in sodium selenite fed chicks (P < 0.05). Both Se sources influenced thyroid hormone serum concentrations (P < 0.05). Chicks fed SY exhibited greater retention of Se in the feathers (P < 0.05). Relative bioavailability of selenium yeast compared with SS for the Se content in carcass, feathers, total and Se retention were, 126, 116, 125 and 125%, respectively. SY supplementation resulted in lower liver Se concentration as Se supplementation increased (P < 0.05).

Conclusions

Based on performance traits, the supplemental level of organic Se as SY in organic trace minerals supplement to support the maximal growth of broiler chicks is 0.133 mg Se/kg.
Genome-wide analysis of long noncoding RNA expression profile in nasal mucosa with allergic rhinitis
Authors: Wei Xian , Xu Man , Wang Chao , Fang Shengjian , Zhang Yu , Wang Weihua
Published: April 2021

Background

Long noncoding RNAs (lncRNAs) are involved in a variety of human immune diseases. However, the expression profile and precise function of lncRNAs in allergic rhinitis (AR) remain unknown. In the present study, genome-wide analysis of lncRNA expression was performed in the nasal mucosa tissue and mRNA regulatory relationship was examined among patients with or without AR.

Methods

Microarray assays were performed and the differential expressions of lncRNAs or mRNA were verified through RT-PCR. The lncRNA functions were annotated using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). The potential regulatory relationships between lncRNAs and the co-expressed mRNAs were analyzed using Cytoscape software. The expressions of specific lncRNAs and mRNAs were examined using an in vitro cell model.

Results

A total of 57 lncRNAs and 127 mRNAs were dysregulated in the nasal mucosa tissue of patients with AR, compared to those of patients without AR (fold change > 2.0 and P < 0.05). GO and pathway analysis indicated that the lncRNA–co-expressed mRNAs were enriched in several biological processes and cellular signaling pathways related to AR, such as positive regulation of the integrin biosynthetic process, cell adhesion, and leukocyte transendothelial migration. Some lncRNAs regulated the co-expressed genes in a cis- and/or trans-regulatory manner. Furthermore, allergen exposure significantly increased the expression of lnc-CXCL12-4, CXCL12, and CXCR4 in BEAS-2B cells compared to untreated cells (P < 0.01).

Conclusion

The results of the present study suggest that lncRNAs participate in the biological pathways related to AR. Leukocyte transepithelial migration may be a potential target for lncRNAs to regulate allergic inflammation and CXCL12/CXCR4 axis plays an important role in the inflammatory process of AR.
Correction to: Determination of serum 25-hydroxyvitamin D status among population in southern China by a high accuracy LC-MS/MS method traced to reference measurement procedure
Authors: Cai Zhiliang , Zhang Qiaoxuan , Xia Ziqiang , Zheng Songbai , Zeng Lilan , Han Liqiao , Yan Jun , Ke Peifeng , Zhuang Junhua , Wu Xinzhong , Huang Xianzhang
Published: April 2021

An amendment to this paper has been published and can be accessed via the original article.
Using organization theory to position middle-level managers as agents of evidence-based practice implementation
Authors: Birken Sarah A. , Currie Graeme
Published: April 2021

Middle-level managers (MLMs; i.e., healthcare professionals who may fill roles including obtaining and diffusing information, adapting information and the intervention, mediating between strategy and day-to-day activities, and selling intervention implementation) have been identified as having significant influence on evidence-based practice (EBP) implementation. We argue that understanding whether and how MLMs influence EBP implementation is aided by drawing upon organization theory. Organization theories propose strategies for increasing MLMs’ opportunities to facilitate implementation by optimizing their appreciation of constructs which we argue have heretofore been treated separately to the detriment of understanding and facilitating implementation: EBPs, context, and implementation strategies. Specifically, organization theory encourages us to delineate different types of MLMs and consider how generalist and hybrid MLMs make different contributions to EBP implementation. Organization theories also suggest that MLMs’ understanding of context allows them to adapt EBPs to promote implementation and effectiveness; MLMs’ potential vertical linking pin role may be supported by increasing MLMs’ interactions with external environment, helping them to understand strategic pressures and opportunities; and how lateral connections among MLMs have the potential to optimize their contribution to EBP implementation as a collective force. We end with recommendations for practice and future research.