Cutaneous and paranasal aspergillosis in an immunocompetent patient
Authors: Khatri Mishri Lal , Stefanato Catherine M. , Benghazeil Mohammed , Shafi Mohammed , Kubba Asha , Bhawan Jag 
Published: March 2003

A 26‐year‐old Libyan woman presented with asymptomatic nodulo‐ulcerative skin lesions present for 1 year. Three years prior to presentation, she had experienced a nasal discharge followed by the development of a nodule in the nasal cavity and a plaque on the hard palate. These lesions had gradually increased in size and ulcerated, resulting in perforation of the nasal septum and palate. Two years later, the patient noticed the appearance of skin lesions: a nodule on the right thumb and numerous nodulo‐ulcerative lesions on the extremities. General physical examination was normal with no significant lymphadenopathy. Examination of the oral cavity revealed perforation of the distal nasal septum, with a perforated nodular plaque involving the entire palate, associated with subluxation of the upper incisors (Fig. 1a). On skin examination, multiple firm nodules and nodulo‐ulcerative lesions with a central eschar and raised margins were observed. The lesions ranged in size from 0.5 to 5 cm and were distributed on the right hand and fingers, left upper arm (Fig. 1b), left calf, and right thigh.

(a) Lesion of the palate (arrow) with subluxation of the upper incisors. (b) Numerous nodulo‐ulcerative lesions on the hands and arms

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Routine laboratory investigations (liver function tests, serum calcium, electrolytes, lipid profile, urine and stool culture studies) were normal. Immunoelectrophoresis disclosed normal levels of immunoglobulins IgG, IgA, and IgM. Serologic studies for human immunodeficiency virus (HIV) and syphilis, and a tuberculin test, were all negative. A Giemsa‐stained tissue smear was negative for Leishmania tropica organisms. Radiological studies disclosed a slight haziness of the maxillary sinuses with perforation of the nasal septum. A chest X‐ray was normal.

Histopathologic examination of biopsies taken from both the palate and from ulcerated and nonulcerated skin lesions was performed, and all showed similar findings. The biopsy of a nonulcerated skin lesion showed pseudoepitheliomatous epidermal hyperplasia with neutrophilic microabscesses (Fig. 2a). A dermal diffuse and nodular granulomatous mixed infiltrate of lymphocytes, histiocytes, giant cells, numerous eosinophils, and neutrophilic microabscesses was seen in all tissues examined. Septate hyphae were present both within giant cells and free in the dermis (Fig. 2b). The hyphae were branching at a 45° angle and were positive on periodic acid–Schiff and Grocott methenamine silver stains (Fig. 2c). Fungal culture studies of material taken from an ulcerated skin lesion grew Aspergillus flavus. Blood cultures were negative for Aspergillus sp. or other microorganisms.

Histopathology of a finger lesion showing marked epidermal hyperplasia, and a mixed neutrophilic and granulomatous inflammatory dermal infiltrate with intraepidermal and dermal neutrophilic microabscesses (hematoxylin and eosin, × 306) (a); septate branching hyphae within giant cells (arrow) (× 666) (b); Grocott silver methenamine stain confirming the 45° angle branching characteristic of Aspergillus sp. (× 1080) (c)

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The patient was treated with intravenous amphotericin B, but the medication was discontinued due to her intolerance to the drug. She was subsequently lost to follow‐up.