bluuebunny/crossref_metadata_2025 huggingface dataset:
A Study on Hydrocyclone with Rotating Blades on Overflow Pipe Wall
Authors: Lai Yung Sheng , Fu Chun Wei , Pan Chuan Yi , Wu Rome Ming
Published: September 2011

This study proposed a new type of hydrocyclone. A rotating ring containing one pair of symmetric blades was attached to the overflow pipe. The momentum of the inlet fluid drove the ring/blades to rotate, and the rotating blades swept small-sized particles to the hydrocyclone wall to be discharged from underflow. This design can destroy circulation flow on hydrocyclone roof and make small-sized particles to be separated.
Characterization of Protein Isolates from Defatted Chinese Hickory (Carya Cathayensis Sarg) Kernels
Authors: He Zhi Ping , Mao Lin Chun , Zhou Cun Shan , Wu Feng Hua
Published: September 2011

Chinese hickory (Carya cathayensis Sarg.) kernel protein isolate (CKPI) was isolated from hinese hickory kernel defatted flour (CKDF) by isoelectric precipitation. CKPI was evaluated for chemical composition and selected functional properties. CKPI contained over 72.0% dry weight (DW) of protein. Nitrogen solubility curves for CKPI were pH-dependent in the pH range of 2.0-12.0 with minimum solubility observed at pH 4.0 (9.8%) and maximum solubility at pH 12.0 (89.9%). Minimum emulsifying capacity (EC) and emulsifying stability (ES) of CKPI were observed at pH 4.0. CKPI had a least gelation concentration of (LGC) of 6% (w/v) at pH 4.0. Results indicated that Chinese hickory kernel may be a new protein source with huge exploitation potential after oil extraction process in China.
A Kinetic Model for Astaxanthin Fermentation by Xanthophyllomyces Dendrorhous Zjut46
Authors: Hu Zhong Ce , Jiang Guo Feng , Zheng Yu Guo
Published: September 2011

Astaxanthin, a main carotenoid pigment, has a strong antioxidant activity. A kinetics of astaxanthin fermentation by Xanthophyllomyces dendrorhous was studied in a batch fermentation system. The unstructured models were proposed using the Logistic equation for microbial growth, the Luedeking-Piret equation for astaxanthin production and Luedeking-Piret-like equation for glucose consumption. The production of astaxanthin was partly growth-associated. Compared with the experimental data and calculated data, most of errors were lower than 10 %, it showed that the proposed unstructured models were adequate to describe the fermentation bioprocess of astaxanthin by X. dendrorhous.
Study on the Integrative Utilization of Cyanide Tailings of Polymetallic Sulfide Ore of Gold and Silver
Authors: Liu Xiao Qi , Fang Yong , Jiang Hao , Xiao Yun , Li Le Gui
Published: September 2011

In this paper, based on the experiment on pure minerals, namely, galena, sphalerite and pyrite, it is concluded that the appropriate pH range for galena flotation is 8.5-10.5, that for sphalerite flotation is 7-10.5 and for pyrite is 2.5-6.5. For galena, sphalerite and pyrite, butyl xanthate has a better collecting performance than ammonium dibutyl dithiophosphate. Both Na2SO3 and ZnSO4 have a relatively weak inhibitory effect on galena. As for sphalerite, the mixing of Na2SO3 and ZnSO4 has a better inhibitory effect than when they are used alone, with the best inhibitory effect obtained when the proportion of Na2SO3 and ZnSO4 is 1:2 and their dosages are respectively 100mg/L and 200mg/L. In zinc flotation, CuSO4 demonstrates an apparent activating effect on the inhibited sphalerite with its appropriate dosage at 2*10 -4 mol/L. In actual separation and selection of ore, when the grades of lead, zinc and silver in cyanide tailings are respectively 2.41%, 3.77% and 272g/t, a fairly good index is obtained where the Pb concentrate is of a grade of 33.41% with its silver grade of 4051.57g/t and the grade of Zn concentrate is 59.67% with its silver grade of 798.08g/t.
Molecular Cloning and Tissue-Specific Expression of the Heart-Type Fatty Acid-Binding Protein (H-FABP)
Authors: Zheng Cheng Li , Xu Gang Yi , Wang Dai Hua
Published: September 2011

In order to understand the structure and function of H-FABP gene, reveal the effect on the heart-type fatty acid-binding protein in goat, the cDNA of H-FABP gene was cloned and sequenced from heart of Tianfu goat （an emerging breed） by T-A clone techniques. The structure and function of H-FABP were analyzed by bioinformatics, and the gene expression profile in different tissues was examined by real-time PCR. The results showed that the full sequence of H-FABP cDNA is 650 bp, containing 402 bp coding region (CDS) encoding 133 amino acids. The isoelectric point (pI) of the protein is 6.11, and the molecular weight is 14.7 kDa. There were eight phosphorylational sites in this protein. The secondary structure of the protein was mainly α-helix, random coil and extended strand structure. The deduced amino acid sequence of H-FABP shared significant identity with the H-FABP from other mammals. The phylogenic tree based on H-FABP protein sequence was detected the closest relationship to cattle. A predicted 3D model was constructed by homology modeling. H-FABP mRNA is a broad-spectrum expression gene, which was detected in heart, liver, spleen, lung, kidney, longissimus muscle and leg muscle. In particular, high expression levels of H-FABP mRNA were detected in heart, longissimus muscle and leg muscle, but low expressions were observed in liver tissue.
Highly Enantioselective Hydrolysis of Racemic Isopropyl Tert-Leucinate by Newly Discovered Baclicus Lincheniformis Jx010 for Synthesis of L-Tert-Butyl Leucine
Authors: Jin Jian Zhong , Zhang Jie
Published: September 2011

Newly discovered strain Baclicus lincheniformis JX010 was identified to enantioselective hydrolysis of racemic ethyl tert-leucinate for the synthesis of chiral L-tert-butyl leucine. In the hydrolysis of isopropyl tert-butyl leucinate, the L-tert-butyl leucine was synthesized in 99% ee and 48% conversion. The cells was immobilized on synthetic resin 0501 without pretreatment to increase the enzyme stability. A series of organic cosolvents were investigated the hydrolysis rate and 2% glycerol was considered as the optimized cosolvent. In the hydrolysis of 50 mM isopropyl tert-butyl leucinate, the immobilized cells remained 85%activity with L-tert-butyl leucine in 99% ee after 8 reaction cycles.
Ionizing Irradiation Property of L(+)-Alpha-Phenylglycine in Acid Aqueous Solution
Authors: Shi Wen Yan , Gu Jian Zhong , Jiao Zheng , Wu Wen Jing , Xu Gang , Wu Ming Hong , Lei Jian Qiu , Hu Gu Yu
Published: September 2011

(+)-alpha-phenylglycine are significant contaminants at pharmaceutical intermediates production. To study processes for the destruction of contaminant L(+)-alpha-phenylglycine in acid aqueous solution we have investigated the transient species using both laser flash photolysis. The OH· reaction with L(+)-alpha-phenylglycine process was investigated and formed polymer. Furthermore, the results of steady-state analysis suggested that L(+)-alpha-phenylglycine removal was found to be more efficient with increasing applied dose. L(+)-alpha-phenylglycine, decreased by 44.50%, using a dose of 14kGy.
Dynamic Investigation of Sensitivity and Action Mechanism of Antitumor Drug
Authors: Liu Xuan , Luan Ying , Guo Ai Rong , Deng Hui Hua , Lu Zu Hong
Published: September 2011

Dynamic responses of A549 cells to epirubicin (EPI), daunorubicin (DNR), cisplatin (DDP) and carboplatin (CBP) were monitored by real-time cell electronic sensing (RT-CES) system. A new defined parameter, the detachment duration, was defined to quantitatively characterize drug-cell action mechanism. For DNR, there were two different linear dependent regions of the detachment duration. The rate constant of DNR in lower concentration region (20.4±2.0 h/μM) is much larger than that in higher concentration region (3.2±0.1 h/μM). Moreover, the rate constant of DNR in lower concentration region is close to that of EPI in higher concentration region (17.5±0.5 h/μM). Similar observation occurred in the case of DDP and CBP treatment, and the rate constant is (2.8±0.4)×10 h/μM and (4.6±1.3)×10 h/μM respectively. Taken together, A549 cells are more sensitive to DNR than EPI and to DDP than CBP. This study provides new insight into cell-drug interaction. RT-CES system may play important role in future drug screening, mechanism exploration and clinical therapy.
An Amperometric Immunosensor for α-Fetoprotein Based on Antibody Coated Nano ZrO2-Au-Poly Lysine Composite Membrane Modified Electrode
Authors: Wu Yuan Zhao , Hu Fu Tao , Gan Ning , Hou Jian Guo , Li Tian Hua , Cao Yu Ting , Zheng Lei
Published: September 2011

One novel amperometric immunosensor for α-fetop- rotein was fabricated which was modified by antibody immobilized on nano ZrO2-Au-polyLysine composite membrane. Firstly, the deoxyribonucleic acid – phenoxyacetic acid isoniazid- Schiff base Co(II) complex (DNA-CoL) mixture film was casted on surface of multi carbon nanotubes(MWCNTs) modified glassy carbon electrode(GCE/CNTs/DNA-CoL). Then the composite nanoparticles (ZrO2-Au-pLL) was employed to immobilize the antibody of α-fetoprotein(AFP) to produce the probes(ZrO2-Au-pLL-anti AFP) for AFP. Finally the probes were modified on GCE/CNTs/DNA-CoL through the specific connection between DNA and ZrO2 to form a novel nanocomposite membrane immunosensor for AFP(GCE/CNTs/ DNA-CoL/ZrO2-Au–pLL-anti AFP). The function of immunosensor was investigated by scanning electron microscopy (SEM), cyclic voltammetry(CV), differential pulse voltammety (DPV) and electrochemical impedance spectroscopy experiment (EIS). The results indicated that combining the advantages of the MWNTs-DNA material and ZrO2-Au-pLL-anti AFP nano probes, the immunosensor with excellent sensitivity and selectivity to AFP was prepared successfully. The modified electrode was sensitive to AFP with a linear relationship between 0.05 and 10 ng · mL−1 and a correl- ation coefficient of 0.9905. The detection limit at a signal to noise ratio of 3 was 0.01 ng·mL−1 under the optimal conditions. The described immunosensor preparation and immunoassay methods offer promise for simple and cost-effective analysis of AFP in serum samples.
Production and Characterization of Egg Yolk Antibodies (Igy) against Two Specific Spoilage Organisms (SSO) in Aquatic Products
Authors: Xu Ya Fu , Lin Hong , Sui Jian Xin , Cao Li Min
Published: September 2011

Using whole cells as antigens, specific egg yolk antibodies (IgY) were generated respectively against Shewanella putrefaciens and Pseudomonas fluorescens, two important spoilage bacteria in aquatic products. The obtained IgY exhibited a high and specific affinity to corresponding antigen bacteria. A concentration-dependent antimicrobial activity of the specific IgY was observed in liquid medium. Compared to control groups, the cell number of S. putrefaciens and P. fluorescens was reduced approximately 84.7% and 88.1%, respectively, in the presence of specific IgY (contain salts) at a concentration of 200 mg/mL after 8 h of incubation. Similar growth inhibitory effects were also observed in solid medium, in which the inhibition ratio was calculated as 52.8% and 62.5% for S. putrefaciens and P. fluorescens, respectively, in the presence of specific IgY at a concentration of 100 mg/mL. These results indicated a great potential of specific IgY as a safe and natural antimicrobial agent for aquatic food preservations.
Gallium Nitride for Nuclear Batteries
Authors: Lu Min , Wang Guo , Yao Chang Sheng
Published: September 2011

Gallium Nitride (GaN) PIN betavoltaic nuclear batteries (GB) are demonstrated in our work for the first time. GaN films are grown on sapphire substrates by metalorganic chemical vapor deposition (MOCVD), and then GaN PIN diodes are fabricated by normal micro-fabrication process. Nickel with mass number of 63 (63Ni), which emits β particles, is loaded on the GaN PIN diodes to achieve GB. Current-Voltage (I-V) characteristics shows that the GaN PIN diodes have leakage current of 18 pA at -10V due to consummate fabrication processes, and the open circuit voltage of the GB is estimated about 0.14 V and the short circuit current density is 89.2nAcm-2 . The relative limited performance of the GB is due to thick dead layer and strong backscattering of β particles, Which lead to less energy deposition in GB. However, the conversion efficiency of 1.6% and charge collection efficiency (CCE) of 100% for the GB have been obtained. Therefore, the output power of the GB are expected to greatly increase with thin dead layer and structural surface weakening the backscattering.
Optimizing the Medium for High Yield Production of Antitumor Exopolysaccharide by Medicinal Mushroom
Authors: Yan Pei Sheng , Zhu Guo Zhen , Cao Li Xin , Dong Jing
Published: September 2011

Ypsizigus marmoreus is a newly cultivated medicinal and edible mushroom with higher antitumor activity. In this paper, a uniform design was used to optimize the medium formula with four factors each at six levels, and a regression model was constructed to predict the antitumor exopolysaccharide production. The optimal concentrations for the enhanced exopolysaccharide production were determined as 30% (w/v) glucose, 8.4% (w/v) soybean peptone, 3% (w/v) KH2PO4and 30% (w/v) sweat potato flour. Subsequent experiment confirmed the validity of the model. Under the optimal condition, exopolysaccharide yield reached 3.39g/L in shake-flask culture, which was 3.53 fold higher than that obtained in the basic medium. The results highlight the future application of optimized medium for high yield production of antitumor exopolysaccharide by fermentation of Hypsizigus marmoreus.
Bioactive Peptides from Low Denatured Peanut Dregs: Production and Antihypertensive Activity
Authors: Zhang Yu Hao , Ma Liang , Wang Qiang
Published: September 2011

Hydrolysates of peanut protein from low denatured peanut dregs, which inhibit the angiotensinⅠconverting enzyme (ACE) were prepared by enzymic hydrolysis with Alcalase and N120p that are two proteases available for industrial use. Combination of Alcalase and N120p hydrolyzed peanut proteins most efficiently and the hydrolysates showed the most high activity (IC50=0.548 mg/ml). Sequential ultrafiltration of the hydrolysates with MW cut-off 10, 5 and 1KD resulted in increased activity of each filtrate up to IC50 of 0.255 mg/ml. Sephadex G-15 gel chromatography of the oligopeptides below MW 1KD eluted a peptide fraction of the most potent activity (IC50=0.091 mg/ml). the oligopeptide below MW 1 KD competitively inhibited ACE and it was evaluated for antihypertensive effect in spontaneously hypertensive rats (SHRs) following oral administration. Systolic blood pressure (SBP) significantly decreased after peptide ingestion and higher dose of peanut oligopeptides could not induce occurrence of low blood pressure.
Research on the Soil Pollution and Control of Pb, Cd and Cr in Taiyuan Suburb
Authors: Yang Guo Yi , Wang Ting
Published: September 2011

The rapid population growth in Taiyuan, the ever-increasing industrial production and the never-ending expansion of the city have led to the serious heavy metal pollution of the soil, the deterioration of the soil fertility, the decrease of the grain output and the decline of grain quality and thus severely influencing the environmental quality and obstructing the sustainable economic development. The paper measures the content of such heavy metals as Pb、Cd、and Cr by Tessier's sequential extraction procedure in different depths of Taiyuan suburban soil, then determines the existing forms of these metals in the soil, discusses the factors influencing the forms of the heavy metals and their migration and transformation, probes into the ecological influence of the heavy metal pollution of soil, and finally the writer puts forward some measures to prevent from such pollution.
Mechanism of Petroleum Hydrocarbon Contaminated Groundwater Remediated by Scoria
Authors: Zhang Wen Jing , Zhang Yu Ling , Jin Huan Chi
Published: September 2011

Scoria which is a kind of natural light bone material was selected as the reaction media of permeable reactive barrier (PRB) in this study. The aim is to investigate the scoria’s adsorption kinetics, adsorption isothermal model and reaction mechanism during the process of its purification to petroleum hydrocarbon contaminated groundwater. Parallel batch tests showed that the removal efficiency of TPH (total petroleum hydrocarbons) was 87.91% when the experiment time to 90 min. The adsorption of scoria to TPH was well fitted by the pseudo-second order kinetic equation and Langmuir isotherm, with the kinetic formula t/Qt=2.3082t+20.692 and the isothermal equation 1/Qe=6.5644/Ce+0.0007, respectively. It was also shown that the apparent maximum adsorption capacity of scoria was 1.7g/g. Analysis results from SEM, IR, XRD and other methods indicated that scoria’s composition and its material structure were closely related to the remediation mechanism of petroleum hydrocarbon contaminated groundwater. However, it was not only physical adsorption but also chemical adsorption happened during scoria’s adsorption process. The results could provide a reliable reaction medium for PRB technology which used in petroleum hydrocarbon contaminated groundwater remediation.
Inhibitory Effects of 30 Kinds of Chinese Herbal Medicine on Fungi in Food
Authors: Ren Shun Cheng , Fan Yong Chao , Li Cui Cui
Published: September 2011

This research aimed to study the effects of 30 kinds of Chinese herbs on fungi in food. 30 kinds of Chinese herbs and 6 common fungi in food were used as experiment material. The effects of water, ethanol absolute and petroleum ether extracts on fungi were studied by filter paper method. The results showed that water extracts of Coptis chinensis. and Magnolia officinalis Rehd.et Wils., the ethanol extracts of Syzygium aromaticum Thunb., Coptis chinensis, Cinnamomum cassia Presl and Cassia Bark, the petroleum ether extracts of Syzygium aromaticum Thunb. and Cassia Bark had strong antimicrobial ability. The study can provide the scientific basis for exploring natural food preservatives.
Studies on Energy Transfer between Phycobiliproteins and Thylakoid Complex
Authors: Li Ye
Published: September 2011

The different concentrations C-phycocyanin (C-PC), Allophycocyanin (APC) or R-phycoerythrin(R-PE) solution can reconstitute with thylakoid to synthesize different donor-acceptor complexes. The energy transfer efficiencies of those complexes are studied, where C-PC, APC and R-PE are donor, the thylakoid are acceptor. The results show excitation energy can be transfered to thylakoid from C-PC or APC. However, the energy transfer process of R-PE to thylakoid membrane does not happen. The energy transfer process of the APC to thylakoid membrane shows the highest efficiency. Those results also indicate that the linkage between phycobiliproteins and thylakoid does not have specificity. It can be concluded that the energy transfer efficiencies not only strongly depend on the relative positions of donor and acceptor but also on spectral matching relation between phycobiliproteins and Chla molecules in thylakoid membrane.
Expression, Purification and Characterization of the Recombinant Hirudin Variant iii in the Bacillus Subtilis
Authors: Chen Hua You , Qi Xiang Hui , Geng Xu , Xu Qing Gang , Wang Jing , Wu Zi Rong
Published: September 2011

Hirudin is the most potent natural inhibitor of thrombin and a powerful anticoagulant. Large-scale production of recombinant hirudin is desirable for therapy. In this study, the gene encoding hirudin variant III was redesigned and synthesized by usingBacillus subtilispreferred codons, and the recombinant hirudin variant III (rHV3) was overexpressed inB. subtilisDB403 with strong anticoagulation activity for the first time. The hirudin activity from the supernatant of culture with optimized expression conditions could reach 210 ATU/ml. The protein in culture supernatant was precipitated by trichloroacetic acid, then desalted by ultrafiltration and purified by anion exchange chromatography. Strong anion Q F.F. performed better than weak anion DEAE F.F. The proper pH and conductivity was determined at pH 8 and 6 ms/cm, respectively. The maximum applied sample was 240 ATU/ml to medium of strong anion Q F.F. This optimized procedure was employed in strong anion exchange HiPrep 16/10Q with the 90% recovery rate and 70.2% purity. After gel filtration, the purity of rHV3 checked by HPLC could reach 95.1%, and the recovery rate was 93% for this step. The purified recombinant rHV3 showed a single band in SDS-PAGE. The rHV3 was stable at 100 °C and acidity condition, but was unstable under the condition of both heating and alkalinity. In conclusion, theses studies suggests thatB.subtilismight be useful for the production of biologically active medicine peptides in secretion facilitating purification procedures, and that this isolation method was suitable for scale-up purification process at a low cost.
Effect of the Combined Probiotics on Inhibiting Pathogenic  Escherichia coli Prolification
Authors: Yin Qing Qiang , Fan Guo Ge , Chang Juan , Zuo Rui Yu , Zheng Qiu Hong
Published: September 2011

The study on the combined probiotics to replace antibiotics becomes more and more important because the by-effects and appearance of “superbug” from abuse of antibiotics in human life and animal production. In order to get the effective combined probiotics for replacing antibiotics and inhibiting pathogenicEscherichia coli(E. coli) prolification, 3 strains ofBacillus subtilis(B. subtilis, named as B1, B2 and B3) were used to determine the pre-combination with experimental design (3 factors×4 levels), and then the pre-combination ofB. subtiliswas combined withLactobacillus casei(L. casei) andHansenula anomala(H. anomala) to achieve the most effective combined probiotics with the same design. The results indicated that the pre-combination ratio of B1, B2 and B3 was 0:1:1, and the most effective combination ratios of the pre-combination,L. caseiandH. anomalawere 0:1:1, 1:3:0, 2:2:3, 2:3:2, respectively (P<0.05). In addition, the incubating groups containingL. caseihad better effect on inhibitingE. coliprolification than those withoutLactobacillus, indicating thatLactobacillushad the best effect on inhibitingE. coliprolification, compared with other bacteria.
Cloning and Characterization of Goose PPARγ Gene Comparison with other Vertebrates
Authors: Wu H.L. , Kong Fan Li , Qiu M.H. , Zhou Bo , Luo J. , Wang H.Y. , Liu Y. , Zhu Qing , He Da Qian
Published: September 2011

The peroxisome proliferator-activated receptor gamma (PPARγ) is a nuclear receptor involved in the transcriptional control of energy, lipid, and glucose homeostasis. In this study, we cloned goose PPARγ gene. The amplified fragment contains coding region sequence with 1731 nucleotides, which putatively codes for 453 amino acids (AA). The homology of nucleotide sequences is from 81.4% to 99.9% among the twelve vertebrates, while the similarity of amino acid sequence ranged from 91.8% to 99.8%. Results showed that the PPARγ gene is conservative among different species. This work constructed the basis for further research on the molecular nature and genetic markers of PPARγ for metabolism traits in goose.
Construction and Vaccine Studies on a Pseudorabies Virus with Deletions in Glycoprotein I and Glycoprotein E Genes
Authors: Zhang Song Lin , Jin Mei Lin , Chen Huan Chun
Published: September 2011

A pseudorabies virus (PRV) mutant with deletions in genes for glycoprotein I (gI) and glycoprotein E (gE) was constructed and evaluated as a candidate vaccine strain for its protective by virulent wildtype (wt) PRV Ea strain challenge in pigs. Transfer plasmid pIESE was constructed and co-transfected with PRV genome into IBRS-2 cells to generate a recombinant mutant PRV Ea gI-/gE- virus. The recombinant virus was confirmed by Southern blotting and indirect immunofluorescence assay (IFA). In animal experiments, the immunogenicity was tested by PRV-enzyme-linked immunosorbent assay (ELISA) and PRV neutralizing assay. PRV Ea gI-/gE- elicited significant humoral immune responses to wt PRV Ea, and PRV gI-/gE- immunization protected pigs against a lethal challenge by virulent PRV Ea strain. These results suggested that the recombinant PRV Ea gI-/gE- might be considered as a potential candidate vaccine against PRV.
Preparation and Characterization of Nano-β-Tricalcium Phosphate/Silk Fibroin Scaffold for Tissue Engineering Applications
Authors: Ou Jun , Jiang Yu Min , Zhang Zhan He
Published: September 2011

Silk fibroin (SF) and β-tricalcium phosphate (β-TCP) had been used in biomedical applications for these years. The potential of silk and β-TCP for application in tissue engineering is currently being explored. The purpose of this study was to prepare and characterize a 3D scaffold consisting of nano-β-TCP/SF composite. XRD and FT-IR analysis showed that predominant crystalline phase of calcium phosphate was β-TCP; a chelate effect between SF and Ca2+ was happened at complexing period of SF and β-TCP. The compressive strength of nano-β-TCP/SF composite was 42 MPa ± 0.12 MPa. In vitro cell cultivation experiment showed that the composite was a good matrix for the growth of osteoblasts. Conclusion: the incorporation of SF into nano-β-TCP can enhance both mechanical strength and bioactivity of the scaffold, which suggests that the β-TCP/SF composite may be a potential biomaterial for tissue engineering.
Investigation and Exploitation on the Experiment Platform for the Medical Ni-Ti Memory Alloy Clutching Internal Fixator
Authors: Chen Guo Ping , Yang Ying , Zou Yuan Wen , Huang Xue Jin
Published: September 2011

How to choose instrument appropriately, especially in the process of fracture internal fixation healing, is always a Gordian knot. Although the resistive action between the bone tissue and SMA (shape memory alloy) internal fixation material is not totally visible, the resistance has a very important influence on the healing of the bone tissue. Based on the phenomena above and the consequences arise from the treatment in the use of the clutching internal fixator, an experiment platform is designed in this article which can make an intensive study of the mechanics properties of the clutching internal fixator, in order to find out the embracing force of clutching internal fixator in the clinical cure. This platform can accurately grasp the distribution of the embracing force when the clutching is under the working condition, and it provides more scientific evidence for the clutching internal fixator which uses in clinical application.
Characteristics of Lysozyme Adsorption on Magnetic Fe3O4/Chitosan Nanoparticles at Fixed pH
Authors: Lin Yan Lan , Wang Ren , Ma Wei , Cheng Zi Hong
Published: September 2011

Magnetic Fe3O4/chitosan nanoparticles were synthesized for lysozyme separation from solution. The adsorption of lysozyme was investigated on magnetic Fe3O4/chitosan nanoparticles at fixed pH 6.0, because the enzymatic activity of lysozyme reaches its maximum in this condition. The influence of initial lysozyme concentration, temperature and contact time on lysozyme adsorption was studied. The results of lysozyme adsorption indicated that the adsorption isotherm fitted Sips model well. The maximum adsorption capacity was 144.11mg/g at 310 K. The thermodynamic parameters, ΔG0, ΔH0 and ΔS0, illustrated that the adsorption of lysozyme was endothermic and spontaneous process.
Surface Characteristic of Pyrrhotite Bio-Oxidized by Acidithiobacillus Ferrooxidans
Authors: Lei Jiang
Published: September 2011

This paper deals with the surface characteristic of pyrrhotite bio-oxidized byAcidithiobacillus ferrooxidans. Large amounts of jarosite and element sulfur were determined in the bio-oxidation processe of pyrrhotite. More complicatedly, biofilm exists on the surface of pyrrhotite. This type of structured community ofA. ferrooxidanswas enclosed in the extracellular polymeric substances (EPS), and covered with the deposition generated in the bio-oxidation processe of pyrrhotite.
NR2B-siRNA Mediated by Hydroxyapatite Nanoparticles Relieves for Malin-Induced Pain of Mice
Authors: Gu Yong Hong , Yan Xue Bin , Huang Dong , Han Rui , Wu Li Xiang
Published: September 2011

To observe the effect of NR2B-siRNA mediated by hydroxyapatite nanoparticles (HA) on formalin-induced inflammatory pain of mice and the expression of NR2B in spinal cord. To preliminarily investigate the feasibility of HA as siRNA carrier to transfer NR2B-siRNA in vivo. The sequence-specific NR2B-siRNA of mice was designed and synthesized initially. Using HA as a siRNA carrier, green fluorescent protein(GFP)-siRNA as the control, 4 ug of NR2B-siRNA was administered into subarachnoid space of mice via conscious injection. On 7th day after intrathecal injection, formalin test was observed for 1 hour in each group, followed by dissection of lumbar segments of spinal cords immediately for use in immunohistochemical staining of NR2B. The results show that NR2B-siRNA not only significantly abolish the nociceptive response of mice in the tonic phase induced by formalin, but also decrease the amount of cells expressing NR2B protein in spinal cord, while GFP-siRNA mediated by HA don’t produce the same effects, which demonstrates that HA is capable of effectively transfering NR2B-siRNA via intrathecal injection, furthermore, HA/NR2B-siRNA complex can significantly reduce formalin-induced pain of mice, and specificly inhibit NR2B expression in spinal cord of mice.
Analysis of the Extraction Effects of Epothilones with Different Organic Solvents
Authors: Zhao Lin , Dai Ji Lin , Wang Jia Yi , Li Ya Wei , Liu Xin Li
Published: September 2011

Epothilones are a kind of poliketide macrolide with antifungal and anticancer bioactivity which are attracting more and more attention. In this paper we compared the extraction effects of epothilones with several organic solvents by comparing the recovery rates and purities. Result shows that chloroform and dichloromethane are more suitable for the extraction of epothilones than methanol which is universally used. Then we analyzed the HPLC and TLC results of the different solvents extract, and optimized the extraction method by using chloroform and ether. The final recovery rate and purity increase 114.2% and 21.53 times than methanol.
Fuel Ethanol Production from Wet Oxidised Corn Stover by S. Cerevisiae
Authors: Qiang Zhang , Thomsen Anne Belinda
Published: September 2011

In order to find out appropriate process for ethanol production from corn stover, wet oxidation（195°C，15 minutes）and simultaneous sacchariﬁcation and fermentation (SSF) was carried out to produce ethanol. The results showed that the cellulose recovery of 92.9% and the hemicellulose recovery of 74.6% were obtained after pretreatment. 86.5% of cellulose was remained in the solid cake . After 24h hydrolysis at 50°C using cellulase（Cellubrix L）,the achieved conversion of cellulose to glucose was 64.8%. Ethanol production was evaluated from dried solid cake and the hydrolysate was employed as liquid fraction . After 142 h of SSF with substrate concentration of 8% (W/V), ethanol yield of 73.1 % of the theoretical based on glucose in the raw material was obtained by S. cerevisiae(ordinary baker’ yeast) . The corresponding ethanol concentration and volumetric productivity were 17.2g/L and 0.121g/L.h respectively. The estimated total ethanol production was 257.7 kg/ton raw material by assuming consumption of both C-6 and C-5. No obvious inhibition effect occurred during SSF. These instructions give you the basic guidelines for preparing papers for WCICA/IEEE conference proceedings.
Characteristics and Stability of Soil Aggregates in Tea Plantation
Authors: Zheng Zi Cheng , Li Ting Xuan , He Shu Qin
Published: September 2011

Soil aggregate stability as a key indicator of soil structure and erodibility to evaluate soil stability, is a product of interactions between soil environment, management practices, and land use patterns. The objective of this study was to analyze the distribution characteristics, characteristics of fractal features and stability of soil aggregates in tea plantations and eucalyptus plantations of Western Sichuan in China. The dry- and water-stable aggregate size distributions were determined by dry sieving and wet sieving methods. The results showed that soil structural properties in tea and eucalyptus plantations were similar. With increase in depth of soil layer, the aggregate stability of tea plantation soil became stronger. Comparative analysis of dry and wet sieving results showed that most of the aggregates in tea plantation soil were unstable. The soil aggregates >5mm and 0.5-0.25mm in size had higher stability, while those 2-1mm in size had lower stability. For tea plantation soil, the correlation coefficients between aggregate stability index and mean weight diameter, geometric mean diameter, fractal dimension were bigger under wet sieving than those under dry sieving. The results showed that aggregate stability index, mean weight diameter, geometric mean diameter, fractal dimension of water-stable aggregates could characterize soil aggregate stability in tea plantation ideally.
Cr Bioaccumulation and its Effects on Nutrient Elements Uptake and Oxidative Response in Corbicula Fluminea Exposed to Hexavalent Chromium
Authors: Wang Pei Fang , Zhang Song He , Wang Chao , Han Ni Ni
Published: September 2011

ThoughC. flumineais always used as a biomonitor to evaluate the aquatic environment, the adaptive mechanism to chromium is far from clear. In this paper, an acute toxicity experiment was conducted to study the alterations in Cr accumulation, nutrient uptake and oxidative response inCorbicula flumineaexposed to Cr6+. Cr content increased inC. flumineawith the increase of exterior Cr6+concentration while the Cr bioaccumulation factor decreased. The Cr6+application disturbed the uptake of Zn, P, Cu, Fe, and Mn content. Increased malondialdehyde (MDA) and decreased catalase activity were detected in whole body, mantle, viscera and pleopod. The MDA accumulation in these organs is in sequence, viscera > mantal > whole body > pleopod, suggesting viscera is more sensitive to Cr than other organs. The results also indicate that Cr6+stress disturbs the balance of nutrient uptake and causes the lipid peroxidation inC. fluminea.
Study on the Application of Bacillus Coagulans to Purify Aquaculture Water
Authors: Wang Jun , Zhao Lin
Published: September 2011

A concentration of 1.2×108 cfu/mL Bacillus coagulans YX-6 was poured into the shrimp culture ponds with the concentration of 1000mL/667m2, and the water quality such pH, DO, NH+4-N content, NO-2-N content, and the death quantity of the shrimp were studied for 7 days. It was demonstrated that the Bacillus coagulans YX-6 could improve water quality in contrast with the control group. The results indicated that the pouring of Bacillus coagulans YX-6 can help to keep the pH 8.0~8.3, increase the DO content of farming pond, and it degraded the NH+4-N from 1.7 mg/L to 0.35 mg/L, simultaneously, it decrease the NO-2-N from 0.64mg/L to 0.05 mg/L, and it decrease the average death rate of the shrimp as 50%（P<0.05）.
Correction of Baseline Drifts due to the Pressure Changes between Attenuated Total Reflection Prism and Human Skin for Noninvasive Blood Glucose Sensing with Fourier Transform Infrared Spectroscopy
Authors: Wang Dong Min , Lu Qi Peng , Yao Li Jun
Published: September 2011

.For noninvasive blood sensing with attenuated total reflection (ATR)-Fourier Transform infrared spectroscopy(FTIR), the baseline of spectra human skin will drift because of the exitence of pressure changes between ATR prism and human skin . To correct the baseline drifts ,In the paper, the fingertips spectra in mid-infrared were recorded under the special conditions. A line equation was calculated using the last 14 spectra coupled with the linear regression analysis between the absorbance spectra at the 1035cm-1and the 950cm-1. And the corrected absorption intensities, after the removal of the baseline drifts, can be applied for the quantification of glucose concentrations in blood easily with the given method in the paper.
Studies on the Absorption Characteristics of Triglyceride in Serum by near Infrared Spectroscopy
Authors: Wang Dong Min , Yao Li Jun
Published: September 2011

To determine the spectral ranges and extract relevant information from near infrared spectroscopy of serum in the regions of 12000-4000cm-1 when predicting triglyceride concentrations, in this paper, the first three principal components, which represent not less than 94 percents spectral information, were calculated using principal components analysis. According to this, certain spectral ranges, which contained useful absorption information, were determined based on the absorption characteristics of triglyceride and the absorption interference of water in serum. At last, three calibration models were developed coupled with the method of partial least squares regression, the results shows that there are significant improvement preferment compared the calibration based on the whole overtones spectral ranges. The analysis described here provides a method of spectral ranges selectivity by which the interference of other constituents can be eliminated easily and is useful for noninvasive measurements of the biophysical constituents in blood.
Effects of Linoleic Acid on the Growth of Lactobacillus acidophilus F0221
Authors: Li Jing Yan , Zhang Lan Wei , Guo Chun Feng , Du Ming , Zhang Ying Chun , Li Bao Lei
Published: September 2011

The aim of this study was to investigate the partly membrane properties mechanism of action of linoleic acid (LA) against Lactobacillus acidophilus F0221. F0221 strain was treated with LA at different concentrations range from 0.1 to 10 g/L. Inhibition of cells growth was measured by counting the viable cell counts, alteration of permeability was assessed by measuring the leakage of 260 nm absorbing materials and the Methylene Blue Dye. Change of membrane fluidity was measured by 1, 6-diphenyl-1, 3, 5-hexatriene fluorescence. The effects of LA concentration on CLA production were quantified by measuring c9, t11 CLA levels in cultures of F0221 strain through gas chromatography (GC) and the effects of LA pre-treatment on cells growth were observed. The treatment of F0221 cells with LA at concentrations lower 1 g/L was not significant altered cell counts, whereas at LA concentrations of between 1 and 10 g/L decreased the cell counts, significantly. Compared with control, cell membrane permeability and membrane fluidity was also increased gradually when F0221 strain was cultured with 1 to 10 g/L LA. Additionally, conversion rate of LA into CLA was decreased in a dose dependent manner in the presence of 1.0 to 10 g/L LA and pre-treatment of LA inhibition cells growth with the increase of LA concentration. Date from this study support the hypothesis that LA exerts antibacterial actions by altering membrane properties and compromising membrane associated function.
Analytic Method on the Quality of Microbial Enhanced Oil Recovery Based on Fuzzy-AHP
Authors: Zhang Nai Bo , Xue Chen Guang
Published: September 2011

Analyzed the evaluation of microbial enhanced oil recovery quality based on the Fuzzy AHP. Obtained the relative importance weight vector which affect the qualities (or factors) through the Fuzzy AHP. The Fuzzy AHP of microbial enhanced oil recovery in order to evaluate the quality as for example, discovered the factors of Evaluation of microbial enhanced oil recovery quality from the relative importance of factors: Under 50°C the high glial oil viscosity reduced 20%, lower the freezing point of high wax 2.2°C. This result is consistent with microbial enhanced oil recovery reservoir screening criteria proposed by the U.S. oil and energy.
A Scale up Submerging Fermentation to Produce Fibrinolytic Enzyme and Mycelia by Cordyceps militaris
Authors: Guo Jian Hua , Chen Hui Xin , Liu Xiao Lan , Gao Hong , Deng Yong Ping , Xiao Jing , Zhang Juan Kun
Published: September 2011

A 10 and 100 L fermentor was used to produce Fibrinolytic enzyme and mycelia by Cordyceps militaris, which was based on the optimized results of the flasks ferment of submerging fermentation and with the principle of equivalent KLa. Two operating parameters of the ferment, stirring speed and ventilation were determined. The results showed that, under the identical KLa with flasks, the stirring speed and ventilation were 50 r/min and 700 L/h, 100 r/min and 600 L/h, or 150 r/min and 500 L/h in 10 L fermentor; And the stirring speed and ventilation were 100r/min and 3 m3/h or 150 r/min and 2 m3/h in 100 L fermentor. In the experiments, the stirring speed and ventilation were 100 r/min and 600 L/h in 10 L fermentor; the stirring speed was 250 r/min and ventilation was 2 m3/h in 100 L fermentor, the results were consistent with the optimized conditions in flasks, and the production of both fibrinolytic enzyme and mycelia reached the optimized results in flasks. Therefore, these results are important for producing fibrinolytic enzyme and mycelia by a scale-up of Cordyceps militaris fermentation.
Study on the Hypolipidemic Effect and Antioxidant Activity of Seabuckthorn Marc Flavonoids
Authors: Jiao Yan , Zheng Xi Qun , Liu Xiao Lan , Chang Ying , Wang Zhen Yu
Published: September 2011

The hypolipidemic effect and anti-oxidative activity of seabuckthorn marc flavonoids (SMF) on hyperlipidemic rats was investigated. The rats were divided into 5 groups, including normal control group, hyperlipidemic model control group and 3 different doses of SMF groups i.e. 3.0 mg/kg·bw, 30.0 mg/kg·bw, 90mg/kg·bw, respectively. The SMF was given orally for 35 days. After the treatment, the body, fat and liver weights of rats were weighed. The content of serum total triglycerides (TG), total cholesterol (TC), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C) and the activity of lipid metabolism and anti-oxidative enzyme were determined. The levels of serum lipids in hyperlipidemic rats fed with SMF were significantly lower than model control group. The activities of hepatic lipase (HL), lipoprotein lipase (LPL), lipidase (LPS), superoxide dismutase (SOD), glutathione (GSH) in liver were increased, while the contents of malondialdehyde (MDA) were decreased in SMF groups. The results indicate that SMF has significantly hypolipidemic effects and antioxidant activity.
CoMFA Study on Novel Substitute Oxazolidine Derivatives
Authors: Fu Ying , Li Hai Tao , Zhao Qing Shan , Ye Fei
Published: September 2011

A series of novel Substitute oxazolidine derivatives with potential herbicide safener activity has been modeled using a 3D quantitative structure-activity relationships approach known comparative molecular field analysis (CoMFA). The cross-validated q2 of CoMFA was 0.612, non-cross-validated R2 was 0.986, and F-test value was 120.1, respectively, with the biological activity of the compounds was expressed by pRC30 (root length). The results are critically discussed using a variety of statistical parameters. The CoMFA model was satisfactory and would be useful for the future design of new N-dichloroacetyl oxazolidine herbicide safener.
Medium Optimization for the Antibacterial Substances Production from Brevibacillus brevis XDH Using Response Surface Methodology
Authors: Song Zhen , Liu Xun Li , Lu Chang Xu , Yu Jian , Ju Rui Cheng , Zhao Yu Hua , Li Jin Yu , Liu Kai Qi
Published: September 2011

Brevibacillus Brevis XDH is an antagonistic bacterium with high antibacterial activity against many pathogens. Fermentation medium for the production of the antibacterial substances was statistically optimized by Plackett-Burman design, steepest ascent experiments and response surface methodology. The optimized medium was composed of (g/L): glucose 15.6, soybean meal 28.4, starch 6, CaCO3 2 and MgSO4 4. After optimization, the production of antibacterial metabolites increased more than 70% than that of control.
A Novel Algorithm for Path Planning of Mobile Robots in Dynamic Environment
Authors: Ning Xiao Mei , Ma Zhan Chun , Guo Li
Published: September 2011

Aimed to accuracy and convergence of Evolutionary Algorithms for path planning of robots in dynamic environment, this paper integrated cloud model into evolutionary algorithm to improved evolutionary operators. One-dimensional cloud operator was used evolution and mutation, at the same time the previous search results were used. Simulation results show that the method is effective in performance.
Effect of the Venom of the Scorpion Heterometrus liangi on the Expression of P21 and Caspase-3 Gene in Human Kyse-510 Cell
Authors: Xiao K.F. , Zhou J. , Wang Zhi , Fu Wen Hui , Lu Xiang Yang
Published: September 2011

This paper focuses on the effect of the venom of the scorpion Heterometrus liangi on the proliferation of human KYSE-510 cell line and the related molecular mechanism. Marked morpho-logical changes in KYSE-510 cells after treatment with scorpion venom were observed using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Cell appopsis were deternined using flow cytometry. moreover, RT-PCR and Western blot analysis was used to research the different level of p21 and caspase-3 expression. These results suggest that scorpin venom at concentrations of 50–100 ug/mL caused dose- dependent inhibition of KYSE-510 cell proliferation. The ratio of apoptosis and necrosis increased. The effect of venom treated in KYSE-510 cells in p21 gene expression was reinforced, but the caspase-3 gene expression has indistinctive effect. The activity of p21 and caspase-3 protein expression were upregulated after scorpion venom treatment. scorpion venom is to activate the expressions of p21 and caspase-3 protein to cause selective cell apoptosis.
Study on the Properties of Poly(Lactic Acid)/Grafted Starch Composites
Authors: Hu Shan , Gao Hu Liang , Zhang Jun
Published: September 2011

The grafted starch was prepared from corn starch and maleic anhydride. Fourier transform infrared spectroscopy (FTIR) was used to characterize the product. The Poly(lactic acid) (PLA)/grafted starch composites were prepared by means of a melt blending method. The effect of grafted starch content on the properties of the composites was investigated. The morphology of the composites was observed by scanning electron microscopy (SEM). Thermal degradation properties were studied by thermogravimetry (TG). The results indicated that the toughness could be improved obviously by adding suitable content of grafted starch. When 35 wt% grafted starch was added into PLA, the tensile strength decreased to 26.6 MPa from 51.7 MPa for pure PLA, elongation increased from 12.0% to 20.3%, flexural strength decreased from 99.0 MPa to 50.2 MPa, impact strength increased from 4.20 kJ/m2 to 6.28 kJ/m2.
Variable Structure Control of the Brushless DC Motor
Authors: Wang Xu Gang , Li Xin Hua
Published: September 2011

In order to increase the degree of the angular velocity tracking precision of the brushless DC motors control system, the controller is designed using the variable structure control with sliding mode. The mathematical model of the brushless DC motors is built. The sliding mode function includes tracking error and the differential coefficient of the tracking error. In order to alleviate the chattering, the saturation function replaces the sign function. The simulation result show the tracking precision is high and the controller output has not obvious chattering. The controller meets the tracking precision of he brushless DC motors control system.