bluuebunny/crossref_metadata_2025 huggingface dataset:
Ion Channels and Transporters in Inflammation: Special Focus on TRP Channels and TRPC6
Authors: Ramirez Giuseppe A. , Coletto Lavinia A. , Sciorati Clara , Bozzolo Enrica P. , Manunta Paolo , Rovere-Querini Patrizia , Manfredi Angelo A.
Published: July 2018

Allergy and autoimmune diseases are characterised by a multifactorial pathogenic background. Several genes involved in the control of innate and adaptive immunity have been associated with diseases and variably combine with each other as well as with environmental factors and epigenetic processes to shape the characteristics of individual manifestations. Systemic or local perturbations in salt/water balance and in ion exchanges between the intra- and extracellular spaces or among tissues play a role. In this field, usually referred to as elementary immunology, novel evidence has been recently acquired on the role of members of the transient potential receptor (TRP) channel family in several cellular mechanisms of potential significance for the pathophysiology of the immune response. TRP canonical channel 6 (TRPC6) is emerging as a functional element for the control of calcium currents in immune-committed cells and target tissues. In fact, TRPC6 influences leukocytes’ tasks such as transendothelial migration, chemotaxis, phagocytosis and cytokine release. TRPC6 also modulates the sensitivity of immune cells to apoptosis and influences tissue susceptibility to ischemia-reperfusion injury and excitotoxicity. Here, we provide a view of the interactions between ion exchanges and inflammation with a focus on the pathogenesis of immune-mediated diseases and potential future therapeutic implications.
A Ladder-Type Organosilicate Copolymer Gate Dielectric Materials for Organic Thin-Film Transistors
Authors: Kim Dongkyu , Kim Choongik
Published: July 2018

A ladder-type organosilicate copolymer based on trimethoxymethylsilane (MTMS) and 1,2-bis(triethoxysilyl)alkane (BTESn: n = 2–4) were synthesized for use as gate dielectrics in organic thin-film transistors (OTFTs). For the BTESn, the number of carbon chains (2–4) was varied to elucidate the relationship between the chemical structure of the monomer and the resulting dielectric properties. The developed copolymer films require a low curing temperature (≈150 °C) and exhibit good insulating properties (leakage current density of ≈10−8–10−7 A·cm−2 at 1 MV·cm−1). Copolymer films were employed as dielectric materials for use in top-contact/bottom-gate organic thin-film transistors and the resulting devices exhibited decent electrical performance for both p- and n-channel organic semiconductors with mobility as high as 0.15 cm2·V−1·s−1 and an Ion/Ioff of >105. Furthermore, dielectric films were used for the fabrication of TFTs on flexible substrates.
Palatal Protective Stents Prevent Oro-Nasal Fistulas after Surgery for Velopharyngeal Insufficiency: A Preliminary Report
Authors: Chaiyasate Kongkrit , Ysunza Pablo Antonio , Spolyar John , Genova Rafaella , Andrade Peter
Published: July 2018

Background: One of the potential complications of surgery for velopharyngeal insufficiency (VPI) is postoperative oral-nasal fistula (ONF). Reported rates vary from 0 to 60%. Several factors are on account of these disproportionate rates. Objective: The purpose of this study was to describe the use of a palatal protective stent (PPS) to preserve the VPI repair surgical site and to study its effectiveness for decreasing the incidence of postoperative ONF. Materials and Methods: A retrospective study was carried out. All patients undergoing surgery for VPI with complete preoperative and postoperative evaluations including at least one year follow up after surgery from 2012 to 2016 were studied. Some of the patients were operated on using a pre-molded palatal protective stent (PPS). Twenty-seven patients were included in the study group. Most of the patients underwent a customized pharyngeal flap according to findings of imaging procedures. The remaining cases underwent a Furlow palatoplasty. Twelve patients were operated on using PPS. Results: There were no surgical complications during the procedures. ONF was detected in four of the patients operated on without PPS. None of the patients undergoing surgery using PPS demonstrated ONF. All fistulas were located at the soft/hard palate junction. VPI was corrected in 92% of the cases. Conclusion: Although only a reduced number of cases were studied, these preliminary results suggest that using PPS during surgical procedures for correcting VPI is a safe and reliable tool for preventing ONF.
A Virtual Micro-Islanding-Based Control Paradigm for Renewable Microgrids
Authors: Hashmi Khurram , Mansoor Khan Muhammad , Jiang Huawei , Umair Shahid Muhammad , Habib Salman , Talib Faiz Muhammad , Tang Houjun
Published: July 2018

Improvements in control of renewable energy-based microgrids are a growing area of interest. A hierarchical control structure is popularly implemented to regulate key parameters such as power sharing between generation sources, system frequency and node voltages. A distributed control infrastructure is realized by means of a communication network that spans the micro-distribution grid. Measured and estimated values, as well as corrective signals are transmitted across this network to effect required system regulation. However, intermittent latencies and failures of component communication links may result in power imbalances between generation sources, deviations in node voltages and system frequency. This paper proposes a hierarchical control structure to regulate the operation of an islanded AC microgrid experiencing communication link failures. The proposed strategy aims to virtually sub-divide the microgrid into controllable “islands”. Thereafter, active power sharing, frequency and voltage restoration is achieved by competing converter systems through multi-agent consensus. The effectiveness of the proposed methodology has been verified through stability analyses using system wide mathematical small signal models and case study simulations in MATLAB, Simpower systems.
Development of Environmental Long Range RFID Sensors Based on the Modulated Scattering Technique
Authors: Donelli Massimo , Manekiya Mohammedhusen
Published: July 2018

This work presents the development of a wireless sensor system for environmental monitoring based on the modulated scattering technique (MST). The MST sensor probes are based on the scattering properties of small passive antennas and radiate part of the impinging electromagnetic field generated by an interrogating antenna, which also acquires the backscattered signal as information. The MST probes are able to deliver data without radio frequency front end. They use a simple circuit that alternatively terminates the antenna probe on suitable loads to generate a low modulation signal on the backscattered electromagnetic wave. In this work, the MST sensor system for environmental monitoring has been proposed to obtain the best performance in communication range, load efficiency and power harvesting. The MST sensor has been fabricated and assessed in practical scenarios. The proposed prototype, able to operate in X band at 10 GHz and able to provide a communication range of about 15 m, serves as a proof-of-concept. The acquired measurements demonstrate that the prototype is able to measure accurately and send data without radio frequency front end or bulky wired connection with the same efficiency of standard wireless sensors such as radio frequency identifier (RFID) or wireless sensor networks (WSN).
A Novel Decomposition-Optimization Model for Short-Term Wind Speed Forecasting
Authors: Zhou Jianzhong , Sun Na , Jia Benjun , Peng Tian
Published: July 2018

Due to inherent randomness and fluctuation of wind speeds, it is very challenging to develop an effective and practical model to achieve accurate wind speed forecasting, especially over large forecasting horizons. This paper presents a new decomposition-optimization model created by integrating Variational Mode Decomposition (VMD), Backtracking Search Algorithm (BSA), and Regularized Extreme Learning Machine (RELM) to enhance forecasting accuracy. The observed wind speed time series is firstly decomposed by VMD into several relative stable subsequences. Then, an emerging optimization algorithm, BSA, is utilized to search the optimal parameters of the RELM. Subsequently, the well-trained RELM is constructed to do multi-step (1-, 2-, 4-, and 6-step) wind speed forecasting. Experiments have been executed with the proposed method as well as several benchmark models using several datasets from a widely-studied wind farm, Sotavento Galicia in Spain. Additionally, the effects of decomposition and optimization methods on the final forecasting results are analyzed quantitatively, whereby the importance of decomposition technique is emphasized. Results reveal that the proposed VMD-BSA-RELM model achieves significantly better performance than its rivals both on single- and multi-step forecasting with at least 50% average improvement, which indicates it is a powerful tool for short-term wind speed forecasting.
Influence of Temperature on the Microstructure Deterioration of Sandstone
Authors: Shen Yan-Jun , Zhang Yu-Liang , Gao Feng , Yang Geng-She , Lai Xing-Ping
Published: July 2018

Macroscopic properties of sandstone are commonly attributed to the degradation of its microstructure during heating treatment processes. However, few previous studies have focused on comprehensive observations on how the microstructure of sandstone changes with temperature. In this study, a kind of sandstone containing quartz, albite, calcite, and laumontite (little), was collected from Linyi (Shandong Province, China) to observe the microstructure degradation changes with temperature by X-ray diffraction (XRD), Scanning electron microscopy (SEM) and thermo-gravimetric analyses (TGA). Firstly, 10 groups of sandstone samples were heated from 25 °C to 900 °C. Then, some core micro-parameters including lattice constant, full width at half maximum (FWHM), micro-strain, dislocation density, TGA curve changes and failure characteristic of the mineral were analyzed comprehensively. Finally, the underlying mechanism causing the microscopic thermal damage at different temperature intervals was also discussed. The results showed that: (1) quartz, the framework component of this sandstone, underwent an α- to β-phase change over the temperature range from 400 °C to 600 °C. This phenomenon caused the lattice constant, micro-strain, dislocation density and TGA curve to decrease sharply during this interval, leading to the microstructure deterioration of sandstone; (2) calcite underwent a decomposition reaction between 600 °C and 800 °C, and resulted in the XRD pattern peak, lattice constant, micro-strain and TGA curve dropping continuously. It destroyed further the internal microstructure of sandstone and produced numerous inter-granular cracks around quartz crystals; (3) further examination found that the decomposition reactions of minerals presented non-synchronized characteristics due to the different sensitivities of minerals to temperature, which led to thermal stress, thermal fracturing of minerals, and thermal reactions happening in different temperature intervals.
Scaling Rules at Constant Frequency for Resonant Inductive Power Transfer Systems for Electric Vehicles
Authors: Cirimele Vincenzo , Freschi Fabio , Guglielmi Paolo
Published: July 2018

The paper proposes the development of a set of rules for the resizing of inductive power transfer systems with particular attention to the ones dedicated to the charge of electric vehicles. These rules aim at the construction of down-scaled prototypes allowing the study and the design with benefits in terms of costs, time consumption and flexibility. The theoretical results are experimentally validated by comparing a 1.1kW system with its down-scaled version.
City Bus Powertrain Comparison: Driving Cycle Variation and Passenger Load Sensitivity Analysis
Authors: Kivekäs Klaus , Lajunen Antti , Vepsäläinen Jari , Tammi Kari
Published: July 2018

Alternative powertrains are rapidly increasing in popularity in city buses. Hence, it is vitally important to understand the factors affecting the performance of the powertrains in order to operate them on appropriate routes and as efficiently as possible. To that end, this paper presents an exhaustive driving cycle and passenger load sensitivity analysis for the most common city bus powertrain topologies. Three-thousand synthetic cycles were generated for a typical suburban bus route based on measured cycles and passenger numbers from the route. The cycles were simulated with six bus models: compressed natural gas, diesel, parallel hybrid, series hybrid, hydrogen fuel cell hybrid, and battery electric bus. Twenty reference cycles featuring various types of routes were simulated for comparison. Correlations between energy consumption and the various driving cycle parameters and passenger loads were examined. Further analysis was conducted with variance decomposition. Aggressiveness and stop frequency had the highest correlation with the consumption. The diesel bus was the most sensitive to aggressiveness. The parallel hybrid had a lower statistical dispersion of consumption than the series hybrid on the suburban route. On the varied routes, the opposite was true. The performance of the parallel hybrid powertrain deteriorated significantly on cycles with high aggressiveness and stop frequency. In general, the high correlation between aggressiveness and energy consumption implies that particular attention must be paid to limiting high-speed accelerations of city buses.
An Experimental Investigation on Flame Pulsation for a Swirl Non-Premixed Combustion
Authors: Xi Zhongya , Fu Zhongguang , Hu Xiaotian , Sabir Syed Waqas , Jiang Yibo
Published: July 2018

Flame pulsation has a significant effect on combustion, and understanding its oscillatory behavior is important to the combustion community. An experiment was performed to analyze the pulsation characteristics of a swirl non-premixed flame under various parameters. The results showed that as fuel mass flow rate increased, the puffing frequency increased due to the decreased flame radiation fraction, and the puffing amplitude became smaller resulting in a more stable flame. With an increase in combustor pressure, the flickering frequency declined because of the increasing soot radiation, while the flickering amplitude uniformly increased, leading to more deteriorative flame stability. With an increment in mass flow rate of primary air, the puffing frequency decreased due to the enhanced mixing between fuel and primary air. Also, the puffing amplitude had an oscillating relationship with the mass flow rate of primary air. When the exit velocity of the injector was increased, the flickering frequency diminished nearly linearly because of the improving swirl intensity, and the flickering amplitude was approximately unaffected by injector exit velocity. Moreover, the measured puffing frequencies summarized over all cases varied within the range of 3–22 Hz, the predicted values from theoretical models based on non-swirl flame also fell within this range. The puffing frequency of swirl combustion was more sensitive to the variation in operating conditions than that of non-swirl combustion. Additionally, the obtained correlations indicated that the Strouhal number St was proportional to Fr−1.4 (the Froude number) and Re−2.9 (the Reynolds number), respectively.
Transient Analyses and Energy Balance of Air Flow in Road Tunnels
Authors: Król Aleksander , Król Małgorzata
Published: July 2018

The issue of airflow dynamic in a road tunnel is considered in this study and its impact on smoke management and people safety is highlighted. It was an attempt to estimate the time needed to reach a final steady state of airflow when the operation mode of jet fans was switched. The numerical model of the tunnel was solved with the use of Ansys Fluent. To reproduce the decrease of atmospheric pressure with height, relative static pressure was applied using UDF (User Defined Function). The ambient weather conditions were taken into account as well. The wind influence was introduced by the additional component of dynamic pressure applied against one of the tunnel portals also using UDF. There are some theoretical foundations of airflow in a tunnel presented in this paper. The obtained results were compared with the measurements carried out in a real road tunnel and the results obtained while applying the above-mentioned physical model. The main contribution of the presented work is the indication of a relatively high relaxation time of airflow in a tunnel, which could be important when designing the emergency pattern of a ventilation system. Additionally, some considerations of kinetic energy exchange between fan jet and air volume would be helpful when choosing fans for ventilation systems being designed.
Decision Tree-Based Preventive Control Applications to Enhance Fault Ride Through Capability of Doubly-Fed Induction Generator in Power Systems
Authors: Vieira Dione , Nunes Marcus , Bezerra Ubiratan
Published: July 2018

The development of a preventive control methodology to increase the capacity of voltage sag recovery (Fault Ride Through Capability (FRTC)) of a doubly-fed induction generator (DFIG) connected in an electrical network is presented. This methodology, which is based on the decision trees (DT) technique, assists with monitoring and support for security and preventive control, ensuring that wind systems remain connected to the power system even after the occurrence of disturbances in the electric system. Based on offline studies, DT discovers inherent attributes of the FRTC scenario related to electrical system behavior and provides a quick prediction model for real-time applications. From the obtained results, it is possible to check that the DFIG is contributing to a system’s operation security from the availability of power dispatch and participation in the voltage control. It is also noted that the use of DT, in addition to classifying the system’s operational state with good accuracy, also significantly facilitates the operator´s task, by directing him to monitor the most critical variables of the monitored operation state for a given system’s topological configuration.
Integrated Energy and Catalyst Thermal Management for Plug-In Hybrid Electric Vehicles
Authors: Zeng Yuping , Cai Yang , Chu Changbao , Kou Guiyue , Gao Wei
Published: July 2018

With plug-in hybrid electric vehicles (PHEVs), the catalyst temperature is below the light-off temperature due to reduced engine load, extended engine off period, and frequent engine on/off shifting. The conversion efficiency of a three-way catalyst (TWC) and tailpipe emissions were proven to depend heavily on the temperature of the catalyst. The existing energy management strategy (EMS) of the PHEVs focuses on the improvement of fuel efficiency and emissions based on hot engine characteristics, but neglects the effect of catalyst temperature on tailpipe emissions. This paper presents a new EMS that incorporates a catalyst thermal management method. First, an additional cost is established to implement additional constraints on catalyst temperature, and then the global cost function is created using this additional cost and the fuel consumption. Second, we find the global optimal solution using Pontryagin’s minimum principle method, which provides an optimal control policy and state trajectories. Then, based on the analysis of the optimal control policy, an engine on/off filter (eng on/off filter) is introduced to command the engine on/off shifting. This filter plays an important role in adjusting both the energy and catalyst thermal management strategy for PHEVs. Finally, a practical approach based on the eng on/off filter is developed, and a genetic algorithm is applied to optimize the time constants of this filter. Simulation results demonstrate that the proposed approach‘s fuel consumption increased slightly, but the tailpipe emissions of HC (hydrocarbons), CO (carbon monoxide) and NOx (nitrogen oxide) significantly decreased compared with the standard approach.
Estimating Individual Tree Height and Diameter at Breast Height (DBH) from Terrestrial Laser Scanning (TLS) Data at Plot Level
Authors: Liu Guangjie , Wang Jinliang , Dong Pinliang , Chen Yun , Liu Zhiyuan
Published: July 2018

Abundant and refined structural information under forest canopy can be obtained by using terrestrial laser scanning (TLS) technology. This study explores the methods of using TLS to obtain point cloud data and estimate individual tree height and diameter at breast height (DBH) at plot level in regions with complex terrain. Octree segmentation, connected component labeling and random Hough transform (RHT) are comprehensively used to identify trunks and extract DBH of trees in sample plots, and tree height is extracted based on the growth direction of the trees. The results show that the topography, undergrowth shrubs, and forest density influence the scanning range of the plots and the accuracy of feature extraction. There are differences in the accuracy of the results for different morphological forest species. The extraction accuracy of Yunnan pine forest is the highest (DBH: Root Mean Square Error (RMSE) = 1.17 cm, Tree Height: RMSE = 0.54 m), and that of Quercus semecarpifolia Sm. forest is the lowest (DBH: RMSE = 1.22 cm, Tree Height: RMSE = 1.23 m). At plot scale, with the increase of the mean DBH or tree height in plots, the estimation errors show slight increases, and both DBH and height tend to be underestimated.
Chl Fluorescence Parameters and Leaf Reflectance Indices Allow Monitoring Changes in the Physiological Status of Quercus ilex L. under Progressive Water Deficit
Authors: Sancho-Knapik Domingo , Mendoza-Herrer Óscar , Gil-Pelegrín Eustaquio , Peguero-Pina José Javier
Published: July 2018

Extreme droughts and heat events, frequently produced in Mediterranean climates, induce anomalies in the ecosystem–atmosphere CO2 fluxes. In order to mitigate the consequences on forests and agriculture, managers must have a better knowledge of the ecosystem by monitoring plant status. Water status is commonly observed measuring water potential but when the extreme event is over, this parameter cannot show managers the recovery of other physiological processes such as photosynthesis. To address this problem, we have evaluated the Quercus ilex L. water status and photosynthetic capacity throughout an intense water scarcity event and a subsequent re-watering. Photosynthetic capacity was evaluated through chlorophyll fluorescence parameters and leaf reflectance indices. We found that all fluorescence parameters changed as water potential decreased and they did not completely recover after re-watering. Among the reflectance indices, the physiological reflectance index (PRI) varied similarly to fluorescence, obtaining a strong correlation with the non-photochemical quenching (NPQ). We proposed using PRI to detect the level of photosynthetic capacity in Q. ilex, as it is much easier-to-handle. We also concluded that intense droughts and heat stress not only might reduce photosynthetic capacity through changes in Chl fluorescence parameters during the stress period, but might also affect photosynthetic capacity once the plant water status is recovered.
Transcriptome Analysis of Adipose Tissue Indicates That the cAMP Signaling Pathway Affects the Feed Efficiency of Pigs
Authors: Xu Yueyuan , Qi Xiaolong , Hu Mingyang , Lin Ruiyi , Hou Ye , Wang Zhangxu , Zhou Huanhuan , Zhao Yunxia , Luan Yu , Zhao Shuhong , Li Xinyun
Published: July 2018

Feed efficiency (FE) is one of the main factors that determine the production costs in the pig industry. In this study, RNA Sequencing (RNA-seq) was applied to identify genes and long intergenic non-coding RNAs (lincRNAs) that are differentially expressed (DE) in the adipose tissues of Yorkshire pigs with extremely high and low FE. In total, 147 annotated genes and 18 lincRNAs were identified as DE between high- and low-FE pigs. Seventeen DE lincRNAs were significantly correlated with 112 DE annotated genes at the transcriptional level. Gene ontology (GO) analysis revealed that DE genes were significantly associated with cyclic adenosine monophosphate (cAMP) metabolic process and Ca2+ binding. cAMP, a second messenger has an important role in lipolysis, and its expression is influenced by Ca2+ levels. In high-FE pigs, nine DE genes with Ca2+ binding function, were down-regulated, whereas S100G, which encodes calbindin D9K that serve as a Ca2+ bumper, was up-regulated. Furthermore, ATP2B2, ATP1A4, and VIPR2, which participate in the cAMP signaling pathway, were down-regulated in the upstream of lipolysis pathways. In high-FE pigs, the key genes involved in the lipid biosynthetic process (ELOVL7 and B4GALT6), fatty acid oxidation (ABCD2 and NR4A3), and lipid homeostasis (C1QTNF3 and ABCB4) were down-regulated. These results suggested that cAMP was involved in the regulation on FE of pigs by affecting lipid metabolism in adipose tissues.
Somatostatin Analogue Treatment Primarily Induce miRNA Expression Changes and Up-Regulates Growth Inhibitory miR-7 and miR-148a in Neuroendocrine Cells
Authors: Døssing Kristina B. V. , Kjær Christina , Vikeså Jonas , Binderup Tina , Knigge Ulrich , Culler Michael D. , Kjær Andreas , Federspiel Birgitte , Friis-Hansen Lennart
Published: July 2018

Somatostatin (SST) analogues are used to control the proliferation and symptoms of neuroendocrine tumors (NETs). MicroRNAs (miRNA) are small non-coding RNAs that modulate posttranscriptional gene expression. We wanted to characterize the miRNAs operating under the control of SST to elucidate to what extent they mediate STT actions. NCI-H727 carcinoid cell line was treated with either a chimeric SST/dopamine analogue; a SST or dopamine analogue for proliferation assays and for identifying differentially expressed miRNAs using miRNA microarray. The miRNAs induced by SST analogue treatment are investigated in carcinoid cell lines NCI-H727 and CNDT2 using in situ hybridization, qPCR and proliferation assays. SST analogues inhibited the growth of carcinoid cells more potently compared to the dopamine analogue. Principal Component Analysis (PCA) of the samples based on miRNA expression clearly separated the samples based on treatment. Two miRNAs which were highly induced by SST analogues, miR-7 and miR-148a, were shown to inhibit the proliferation of NCI-H727 and CNDT2 cells. SST analogues also produced a general up-regulation of the let-7 family members. SST analogues control and induce distinct miRNA expression patterns among which miR-7 and miR-148a both have growth inhibitory properties.
Pain-Associated Transcriptome Changes in Synovium of Knee Osteoarthritis Patients
Authors: Bratus-Neuenschwander Anna , Castro-Giner Francesc , Frank-Bertoncelj Mojca , Aluri Sirisha , Fucentese Sandro , Schlapbach Ralph , Sprott Haiko
Published: July 2018

Joint pain causes significant morbidity in osteoarthritis (OA). The aetiology of joint pain in OA is not well understood. The synovial membrane as an innervated joint structure represents a potential source of peripheral pain in OA. Here we analyse, using a hypothesis-free next generation RNA sequencing, the differences in protein-coding and non-coding transcriptomes in knee synovial tissues from OA patients with high knee pain (n = 5) compared with OA patients with low knee pain (n = 5), as evaluated by visual analogue scale (VAS). We conduct Gene Ontology and pathway analyses on differentially expressed mRNA genes. We identify new protein-coding, long non-coding RNA and microRNA candidates that can be associated with OA joint pain. Top enriched genes in painful OA knees encode neuronal proteins that are known to promote neuronal survival under cellular stress or participate in calcium-dependent synaptic exocytosis and modulation of GABA(γ-aminobutyric acid)ergic activity. Our study uncovers transcriptome changes associated with pain in synovial microenvironment of OA knees. This sets a firm ground for future mechanistic studies and drug discovery to alleviate joint pain in OA.
Functionality in Middle-Aged and Older Overweight and Obese Individuals with Knee Osteoarthritis
Authors: Akhavan Neda S. , Ormsbee Lauren , Johnson Sarah A. , George Kelli S. , Foley Elizabeth M. , Elam Marcus L. , Ezzat-Zadeh Zahra , Panton Lynn B. , Arjmandi Bahram H.
Published: July 2018

Patients with knee osteoarthritis (OA) suffer from immobility and pain. The objective of this cross-sectional study was to investigate the relationship between pain and functionality in middle-aged and older overweight and obese individuals with mild-to-moderate knee OA. Overall pattern, physical activity, and total energy expenditure (TEE) were assessed in 83 participants. The Western Ontario McMaster Universities Arthritis Index (WOMAC) was used to assess lower extremity pain and function. The six-minute walk test (6-MWT) and range of motion (ROM) were also assessed. Results indicated that age was inversely associated with body mass index (BMI) (r = 0.349) and total WOMAC scores (r = 0.247). BMI was positively associated with TEE (r = 0.430) and WOMAC scores (r = 0.268), while ROM was positively associated with the 6-MWT (r = 0.561) and negatively associated with WOMAC (r = 0.338) and pain scores (r = 0.222). Furthermore, women had significantly greater WOMAC scores (p = 0.046) than men. Older participants (≥65 years old) had significantly lower BMI (p = 0.002), and distance traveled during the 6-MWT (p = 0.013). Our findings indicate that older individuals in this population with knee OA had lower BMI, greater ROM, and less pain and stiffness and walked slower than middle-aged individuals. Women reported greater pain, stiffness, and reduced functionality, indicating that the manifestation of OA may vary due to gender.
Self-Assembled Antimicrobial Nanomaterials
Authors: Carmona-Ribeiro Ana Maria
Published: July 2018

Nanotechnology came to stay improving the quality of human life by reducing environmental contamination of earth and water with pathogens. This review discusses how self-assembled antimicrobial nanomaterials can contribute to maintain humans, their water and their environment inside safe boundaries to human life even though some of these nanomaterials display an overt toxicity. At the core of their strategic use, the self-assembled antimicrobial nanomaterials exhibit optimal and biomimetic organization leading to activity at low doses of their toxic components. Antimicrobial bilayer fragments, bilayer-covered or multilayered nanoparticles, functionalized inorganic or organic polymeric materials, coatings and hydrogels disclose their potential for environmental and public health applications in this review.
Historical Collaborative Geocoding
Authors: Cura Rémi , Dumenieu Bertrand , Abadie Nathalie , Costes Benoit , Perret Julien , Gribaudi Maurizio
Published: July 2018

The latest developments in the field of digital humanities have increasingly enabled the construction of large data sets which can be easily accessed and used. These data sets often contain indirect spatial information, such as historical addresses. Historical geocoding is the process of transforming indirect spatial information into direct locations which can be placed on a map, thus allowing for spatial analysis and cross-referencing. There are many geocoders that work efficiently for current addresses. However, these do not tackle temporal information, and usually follow a strict hierarchy (country, city, street, house number, etc.) which is difficult—if not impossible—to use with historical data. Historical data is filled with uncertainty (pertaining to temporal, textual, and positional accuracy, as well as to the reliability of historical sources) which can neither be ignored nor entirely resolved. Our open source, open data, and extensible solution for geocoding is based on extracting a large number of simple gazetteers composed of geohistorical objects, from historical maps. Geocoding a historical address becomes the process of finding one or several geohistorical objects in the gazetteers which best match the historical address searched by the user. The matching criteria are customisable, weighted, and include several dimensions (fuzzy string, fuzzy temporal, level of detail, positional accuracy). Since our goal is to facilitate historical work, we also put forward web-based user interfaces which help geocode (one address or batch mode) and display results over current or historical maps. Geocoded results can then be checked and edited collaboratively (no source is modified). The system was tested on the city of Paris, France, for the 19th and 20th centuries. It showed high response rates and worked quickly enough to be used interactively.
Transmembrane TNF and Partially TNFR1 Regulate TNFR2 Expression and Control Inflammation in Mycobacterial-Induced Pleurisy
Authors: Uysal Husnu , Chavez-Galan Leslie , Vesin Dominique , Blaser Guillaume , Benkhoucha Mahdia , Ryffel Bernhard , Quesniaux Valérie F. J. , Garcia Irene
Published: July 2018

Pleural tuberculosis is one of the most frequent forms of extra-pulmonary tuberculosis observed in patients infected with Mycobacterium tuberculosis. Tumor Necrosis Factor (TNF) is a crucial cytokine needed to control tuberculosis infection that remains a leading cause of morbidity and mortality worldwide. TNF blockade compromises host immunity and may increase the risk of reactivation of latent infection resulting in overt pulmonary, pleural and extra-pulmonary tuberculosis. While TNF signaling is mainly considered pro-inflammatory, its requirement for the anti-inflammation process involved in the resolution of infection and tissue repair is less explored. Our study analyzes the role of TNF and TNF receptors in the control of the inflammatory process associated with Bacillus Calmette-Guérin (BCG)-induced pleurisy. This study shows that the absence of TNF causes exacerbated inflammation in the pleural cavity of BCG-infected mice which is controlled by the transmembrane TNF (tmTNF) expression. The lack of TNF is associated with an impaired cellular expression and shedding of TNFR2 in the pleural cavity. The presence of tmTNF restores the normal expression of TNFR2 on myeloid cells during BCG-induced pleurisy. We also show that absence of TNFR1 affects the expression of TNFR2 on pleural cells and inflammation in the pleural cavity of BCG-infected mice. In conclusion, tmTNF but not soluble TNF prevents pleural cavity inflammation leading to attenuation and the resolution of the inflammatory process caused by mycobacterial pleurisy in association with the expression of TNFR2 on myeloid cells.
Optimized Treatment and Recovery of Irradiated [18O]-Water in the Production of [18F]-Fluoride
Authors: Uhlending Antje , Henneken Harald , Hugenberg Verena , Burchert Wolfgang
Published: July 2018

Enriched [18O]-water is the target material for [18F]-fluoride production. Due to its high price and scarce availability, an increased interest and necessity has arisen to recycle the used water, in order to use it multiple times as a target material for [18F]-fluoride production. This paper presents an efficient treatment and reprocessing procedure giving rise to high chemical quality [18O]-water, thereby maintaining its enrichment grade. The reprocessing is subdivided into two main steps. In the first step, the [18F]-FDG (fluorodeoxyglucose) synthesis preparation was modified to preserve the enrichment grade. Anhydrous acetonitrile is used to dry tubing systems and cartridges in the synthesis module. Applying this procedure, the loss in the enrichment throughout the reprocessing is <1%. The second step involves a fractional distillation in which the major part of the [18O]-water was recycled. Impurities such as solvents, ions, and radioactive nuclides were almost completely separated. Due to the modified synthesis preparation using acetonitrile, the first distillation fraction contains a larger amount of an azeotropic [18O]-water/acetonitrile mixture. This fraction is not further distillable. Contents of the remaining [18O]-water were separated from the azeotropic mixture by using a molecular sieve desiccant. This process represents a fast, easy, and inexpensive method for reprocessing used [18O]-water into new [18O]-water quality for further application.
Identification of Antifungal Targets Based on Computer Modeling
Authors: Bencurova Elena , Gupta Shishir K. , Sarukhanyan Edita , Dandekar Thomas
Published: July 2018

Aspergillus fumigatus is a saprophytic, cosmopolitan fungus that attacks patients with a weak immune system. A rational solution against fungal infection aims to manipulate fungal metabolism or to block enzymes essential for Aspergillus survival. Here we discuss and compare different bioinformatics approaches to analyze possible targeting strategies on fungal-unique pathways. For instance, phylogenetic analysis reveals fungal targets, while domain analysis allows us to spot minor differences in protein composition between the host and fungi. Moreover, protein networks between host and fungi can be systematically compared by looking at orthologs and exploiting information from host–pathogen interaction databases. Further data—such as knowledge of a three-dimensional structure, gene expression data, or information from calculated metabolic fluxes—refine the search and rapidly put a focus on the best targets for antimycotics. We analyzed several of the best targets for application to structure-based drug design. Finally, we discuss general advantages and limitations in identification of unique fungal pathways and protein targets when applying bioinformatics tools.
Applying Built-Up and Bare-Soil Indices from Landsat 8 to Cities in Dry Climates
Authors: Rasul Azad , Balzter Heiko , Ibrahim Gaylan R. Faqe , Hameed Hasan M. , Wheeler James , Adamu Bashir , Ibrahim Sa’ad , Najmaddin Peshawa M.
Published: July 2018

Arid and semi-arid regions have different spectral characteristics from other climatic regions. Therefore, appropriate remotely sensed indicators of land use and land cover types need to be defined for arid and semi-arid lands, as indices developed for other climatic regions may not give plausible results in arid and semi-arid regions. For instance, the normalized difference built-up index (NDBI) and normalized difference bareness index (NDBaI) are unable to distinguish between built-up areas and bare and dry soil that surrounds many cities in dry climates. This paper proposes the application of two newly developed indices, the dry built-up index (DBI) and dry bare-soil index (DBSI) to map built-up and bare areas in a dry climate from Landsat 8. The developed DBI and DBSI were applied to map urban areas and bare soil in the city of Erbil, Iraq. The results show an overall classification accuracy of 93% (κ = 0.86) and 92% (κ = 0.84) for DBI and DBSI, respectively. The results indicate the suitability of the proposed indices to discriminate between urban areas and bare soil in arid and semi-arid climates.
Bioremediation of Landfill Leachate with Fungi: Autochthonous vs. Allochthonous Strains
Authors: Spina Federica , Tigini Valeria , Romagnolo Alice , Varese Giovanna Cristina
Published: July 2018

Autochthonous fungi from contaminated wastewater are potential successful agents bioremediation thanks to their adaptation to pollutant toxicity and to competition with other microorganisms present in wastewater treatment plant. Biological treatment by means of selected fungal strains could be a potential tool to integrate the leachate depuration process, thanks to their fungal extracellular enzymes with non-selective catalytical activity. In the present work, the treatability of two real samples (a crude landfill leachate and the effluent coming from a traditional wastewater treatment plant) was investigated in decolorization experiments with fungal biomasses. Five autochthonous fungi, Penicillium brevicompactum MUT 793, Pseudallescheria boydii MUT 721, P. boydii MUT 1269, Phanerochaete sanguinea MUT 1284, and Flammulina velutipes MUT 1275, were selected in a previous miniaturized decolorization screening. Their effectiveness in terms of decolorization, enzymatic activity (laccases and peroxidases), biomass growth and ecotoxicity removal was compared with that of five allochthonous fungal strains, Pleurotus ostreatus MUT 2976, Porostereum spadiceum MUT 1585, Trametespubescens MUT 2400, Bjerkanderaadusta MUT 3060 and B. adusta MUT 2295, selected for their well known capability to degrade recalcitrant pollutants. Moreover, the effect of biomass immobilization on polyurethane foam (PUF) cube was assessed. The best decolorization (60%) was achieved by P. spadiceum MUT 1585, P. boydii MUT 721 and MUT 1269. In the first case, the DP was achieved gradually, suggesting a biodegradation process with the involvement of peroxidases. On the contrary, the two autochthonous fungi seem to bioremediate the effluent mainly by biosorption, with the abatement of the toxicity (up to 100%). The biomass immobilization enhanced enzymatic activity, but not the DP. Moreover, it limited the biomass growth for the fast growing fungi, MUT 721 and MUT 1269. In conclusion, robust and versatile strains coming from well-characterized collections of microorganisms can obtain excellent results comparing and even exceeding the bioremediation yields of strains already adapted to pollutants.
Electron Microscopy and Spectroscopy in the Analysis of Friction and Wear Mechanisms
Authors: Luo Quanshun
Published: July 2018

Friction and wear take place on two solid surfaces in sliding contact as a result of the mechanical, thermal, and chemical interactions with the participation of environmental species. These interactions lead to the formation of a tribo-layer or tribofilm, which attaches on the worn surfaces, and consequently, contributes to the variation of the friction and wear behaviour. Electron microscopy and the associated spectroscopic analyses are powerful in probing these matters in spatial resolutions from micro to atomic scale. This article provides a review of the author’s work in the wear and friction mechanisms of physical vapour deposition (PVD) hard coatings, in which various scanning electron microscope (SEM)- and transmission electron microscope (TEM)-based microscopic and spectroscopic techniques were employed. Understanding on the failure mechanisms and the origin of self-adaptive friction has been improved to the nano-scale. Other related issues are also discussed, such as sample preparation techniques for cross-sectional electron microscopy, energy dispersive X-ray spectroscopy, and electron energy loss spectroscopy.
A Study on Dissimilar Friction Stir Welded between the Al–Li–Cu and the Al–Zn–Mg–Cu Alloys
Authors: Wu Pengfei , Deng Yunlai , Fan Shitong , Ji Hua , Zhang Xinming
Published: July 2018

In this paper, the microstructure of friction stir welded between the Al–Li–Cu alloy and the Al–Zn–Mg–Cu alloy was studied using optical microscope(OM), electronic backscattered diffraction (EBSD), SEM, and TEM. The hardness profile revealed that the range of heat affect zone for the Al–Zn–Mg–Cu alloy was slightly wider than the Al–Li–Cu alloy when they suffered from the thermal transient. Additionally, the characterization of precipitates inside the different zones was obviously different, which corresponded to the microhardness distribution profile. At the periphery of the kissing line, the Al–Li–Cu and the Al–Zn–Mg–Cu alloys mutually diffused during the weld process. The magnesium element in the Al–Zn–Mg–Cu alloy diffused into the Al–Li–Cu alloy. But the copper and zinc had no change because of the low diffusion coefficient in aluminum. The heat affected zone of the Al–Zn–Mg–Cu alloy showed a higher corrosion susceptibility immersed into the corrosion environment, and the Al–Li–Cu alloy did not show severe corrosion.
Optimization of TiC Content during Fabrication and Mechanical Properties of Ni-Ti-Al/TiC Composites Using Mixture Designs
Authors: Lee Dong-Jin , Park Jae-Ha , Kang Myung-Chang
Published: July 2018

Ni-Ti-Al alloys are highly promising materials for use in high-temperature structural materials. However, minimal research has been conducted to improve the associated mechanical properties through secondary phase addition. In this study, Ni-Ti-Al/TiC composites were fabricated at a pressure of 40 MPa and a sintering temperature of 1050 °C using spark plasma sintering. The microstructure and interfacial structure were analyzed by scanning electron microscopy, energy dispersive X-ray spectroscopy, and X-ray diffraction analysis. Microscopic analysis revealed that TiC particles interacted with Ti and Al, resulting in the formation of Ti2AlC, which promoted chemical metallurgical bonding between the Ni–Ti–Al alloy and TiC. Wear characteristics were measured using the wear test with a ball on disk. It was confirmed that the 40 wt % specimen had the highest hardness due to pores generated inside, but the wear amount was relatively high. The mixture design of a minitap was proceeded using hardness, bending strength, and wear loss. An optimum composition ratio of 32.16 wt % was determined using the composite desirability of the three properties.
The Effect of Particle Shape on Sintering Behavior and Compressive Strength of Porous Alumina
Authors: Miyake Kimiya , Hirata Yoshihiro , Shimonosono Taro , Sameshima Soichiro
Published: July 2018

Alumina particles with different shapes, such as sphere, rod, and disk, were examined for the sintering behavior and compressive strength of partially sintered porous alumina. While both the spherical and disk-like particles were packed well to the relative density of 61.2–62.3%, the packing density of rod-like particles was only 33.5%. The sintering rate of alumina particles increased in the order of disk < rod < sphere. The compressive strength of sintered porous alumina was higher for the spherical particles than for the rod-like and disk-like particles. The uniform distribution of the applied load over many developed grain boundaries contributed to the increase in the compressive strength for the spherical particles. The applied load concentrated on a few grain boundaries of rod-like or disk-like particles, caused fracture at a low compressive stress.
Processing Optimization and Characterization of Angiotensin-Ι-Converting Enzyme Inhibitory Peptides from Lizardfish (Synodus macrops) Scale Gelatin
Authors: Chen Junde , Liu Ying , Wang Guangyu , Sun Shanshan , Liu Rui , Hong Bihong , Gao Ran , Bai Kaikai
Published: July 2018

Hypertension can cause coronary heart disease. Synthetic angiotensin-converting enzyme (ACE) inhibitors are effective antihypertensive drugs but often cause side effects. The aim of this study was to prepare potential ACE inhibitors from scales. Gelatin was extracted from lizardfish scales. Then, scale gelatin was enzymolyzed to prepare ACE inhibitory peptides using response surface methodology. Proteolytic conditions after optimization were as follows: pH 7.0, enzyme substrate ratio 3.2%, temperature 47 °C, and proteolysis lasting 2 h and 50 min. The experimental ACE inhibitory activity under optimal conditions was 86.0 ± 0.4%. Among the 118 peptides identified from gelatin hydrolysates, 87.3% were hydrophilic and 93.22% had a molecular weight <2000 Da. Gelatin peptides had high stability upon exposure to high temperature and pH as well as gastrointestinal tract enzymes. Gelatin peptides showed an antihypertensive effect in spontaneously hypertensive rats at a dosage of 2 g/kg in the long-term experiments. A new ACE inhibitory peptide was isolated from gelatin hydrolysates, and was identified as AGPPGSDGQPGAK with an IC50 value of 420 ± 20 μM. In this way, ACE inhibitory peptides derived from scale gelatin have the potential to be used as healthy ACE-inhibiting drug raw materials.