bluuebunny/crossref_metadata_2025 huggingface dataset:
Sign language interpreting services
Authors: De Meulder Maartje , Haualand Hilde
Published: September 2019

Sign language version of the abstract

This article rethinks the impact of sign language interpreting services (SLIS) as a social institution. It starts
from the observation that “access” for deaf people is tantamount to availability of sign language interpreters, and the often
uncritically proposed and largely accepted solution at the institutional level to lack of access seems to be increasing the number
of interpreters. Using documented examples from education and health care settings, we raise concerns that arise when SLIS become
a prerequisite for public service provision. In doing so, we problematize SLIS as replacing or concealing the need for
language-concordant education and public services. We argue that like any social institution, SLIS should be studied and analyzed
critically. This includes more scrutiny about how different kinds of “accesses” can be implemented without SLIS, and more
awareness of the contextual languaging choices deaf people make beyond the use of interpreters.
Arginine-mediated dissociation of single cells and cell sheets from a polystyrene culture dish
Authors: Ikeda Takeshi , Ichikawa Kohei , Shigeto Hajime , Ishida Takenori , Hirota Ryuichi , Funabashi Hisakage , Kuroda Akio
Published: September 2019

Here, we report a novel non-enzymatic cell dissociation method, based on our finding that adherent cells dissociate rapidly from the polystyrene culture dish when incubated in an l- or d-arginine-containing solution. We also demonstrate the successful detachment of confluent NIH/3T3 cell monolayers from the culture dish as a cell sheet by the addition of an arginine solution.
Investigation into the current loss in InAs/GaAs quantum dot solar cells with Si-doped quantum dots
Authors: Chan Shun , Kim Dongyoung , Tang Mingchu , Li Xiao , Liu Huiyun
Published: September 2019

Our previous studies have shown that introducing Si doping in quantum dots (QDs) can help QD solar cells achieve higher voltage. However, this improvement came at the cost of current loss. In this work, we continue to investigate the cause of the current loss and propose a method to recover it without compromising the voltage. Photoluminescence measurements have confirmed that optimizing the thickness of the GaAs layers in the i-region can lead to strong current gain (~14%) with minimal voltage loss (<3%) and alteration of the QD quality. The capacitance–voltage measurement results support that the current gain mainly originates from the increased depletion width.
Magnetic-impurity-induced modifications to ultrafast carrier dynamics in the ferromagnetic topological insulators Sb2−xVxTe3
Authors: Sumida K , Kakoki M , Reimann J , Nurmamat M , Goto S , Takeda Y , Saitoh Y , Kokh K A , Tereshchenko O E , Güdde J , Höfer U , Kimura A
Published: September 2019

Quantum anomalous Hall effect (QAHE) is a key phenomenon for low power-consumption device applications owing to its dissipationless spin-polarized and quantized current in the absence of an external magnetic field. However, the recorded working temperature of the QAHE is still very low. Here we systematically investigate the magnetic dopants induced modifications from the view points of magnetic, structural and electronic properties and the ultrafast carrier dynamics in a series of V-doped Sb2Te3 samples of composition Sb2−x
V
x
Te3 with x = 0, 0.015 and 0.03. Element specific x-ray magnetic circular dichroism signifies that the ferromagnetism of V-doped Sb2Te3 is governed by the p–d hybridization between the host carrier and the magnetic dopant. Time- and angle-resolved photoemission spectroscopy excited with mid-infrared pulses has revealed that the V impurity induced states underlying the topological surface state (TSS) add scattering channels that significantly shorten the duration of transient surface electrons down to the 100 fs scale. This is in a sharp contrast to the prolonged duration reported for pristine samples though the TSS is located inside the bulk energy gap of the host in either magnetic or non-magnetic cases. It implies the presence of a mobility gap in the bulk energy gap region of the host material that would work toward the robust QAHE. Our findings shed light on the material design for low-energy-consuming device applications.
International Conference “Physics of Dispersed Media for Electronics and IT devices” 17-19 April 2019, Moscow, Russia
Authors: Belyaev V V
Published: September 2019

A brief description of scientific program and papers of International Conference “Physics of Dispersed Media for Electronics and IT devices” is presented. It is an annual conference of Moscow Region State University (MRSU).
Studies and applications of magnetic interactions in the system “iron-copper-water”
Authors: Klassen N V , Burova D N , Cebruk I S
Published: September 2019

The recovery of copper by iron in copper sulfate aqueous solutions was studies as a function of the external magnetic field and morphologies of iron containing steel samples. Essential retardation of the recovery by the magnetic field was found as well as decrease of iron sulfate solubility in water induced by the field. The retardation is explained by the active participation of inter-domain walls in the recovery and decreasing of their length in the magnetic field. When steel filings were used for the recovery instead of the volume samples the magnetic retardation was not notices. Applications of these results are developed for new methods of lithography of microschemes and restoration of electrical contact wires.
Laser forming of nanostructures in aqueous suspensions with particular optical and photoelectrical properties
Authors: Vinokurov S E , Klassen N V
Published: September 2019

The self-organizing forming of ordered nanostructures in the aqueous suspensions with inorganic nanoparticles of nickel, copper, silica and bio-organic particles of grinded green leaves were studied by means of the irradiation with red, green and blue lasers. Various forms of self-consistent non-homogeneous distributions of the nanocomponents and the optical flows were found. The suspensions with high density of the nickel nanoparticles demonstrated abrupt changes of their transparency and periodicity. These abrupt changes were transformed to more slow oscillations of the particles and light distributions when the nickel particles were mixed with the nanoparticles of copper. The suspensions with the nanoparticles of copper created arc-like agglomerations explained by generation of vortexes. The vortex-like distributions were found in the suspensions with silica as well. The anisotropic agglomerations were observed during the irradiations of the suspensions with the grinded green leaves. These results are promising for the developments of new kinds of optical modulators and switchers, optical memory and green solar batteries.
Formation of vortex turbulence by a heat current in superfluid helium in a long capillary
Authors: Orlova A A , Efimov V B
Published: September 2019

The propagation of long pulses of the second sound in superfluid helium was experimentally studied. The results of the experiments showed that at the propagation of heat currents of sufficiently high power the process of formation a vortex tangle begins near the heater. It leads to significantly reducing the counterflow of the normal and the superfluid components in helium and, consequently, thermal conductivity of superfluid helium.
An Interference of P-waves from Weakly Conducting and Transparent Dielectric Films
Authors: Yushkanov A A , Zverev N V
Published: September 2019

An influence of kinetic and quantum properties of non-degenerate electron plasma on the interference of P-waves from films of the similar thickness made of weakly conducting substance and of transparent dielectric one, is investigated numerically. The reflection and transmission coefficients of interfering P-waves as well as their phase differences are studied. It is found that for the frequencies of the order of plasma frequency in case of small film thicknesses, the results for quantum non-degenerate electron plasma differ from the ones for classical non-degenerate electron plasma and for classical electron gas. Here one observes a temperature dependence of the values in the cases of quantum and classical non-degenerate electron plasma.
Thermal conductivity performance of nanoporous plate for high-power LED lighting and power electronics
Authors: Kurilov A D , Belyaev V V , Nessemon K D , Besprozvannyi E D , Osin A O , Tokareva O A , Skvortsov A Y
Published: September 2019

Comparative results of measurements of the thermal resistance of a new nanoporous material based on aluminium oxide are presented. Low-cost high-heat-conducting printed circuit boards for high-power LEDs, LED lighting devices and power electronics on the basis of the presented material have been developed and manufactured. Comparison of a set of performances of nanoporous aliminium oxide and aluminium nitride ceramics demonstrate advantages of the material developed. Its thermal characteristics is inferior to the AlN ceramics by 30%, but the cost of the proposed material is lower by almost four times.
Comparative analysis of application of wavelet analysis for the recognition of element composition nanostructures
Authors: Tkachenko I M , Kozhanova E R , Belyaev V V , Yazbeck H
Published: September 2019

The paper presents a technique for applying a wavelet analysis (continuous and discrete wavelet transforms) to analyze the elemental composition of nanostructures in order to identify the chemical composition. The experimental data were obtained by means of a scanning electron microscope. The acquisition, processing and study of spectra were carried out using the author’s software product “labpraktikum_osnov_wavelet”.
Influence of nano-objects positioning on sensitivity of QCM sensor
Authors: Novichkova P O , Vechkanov A R , Yanovitch S V
Published: September 2019

Dependence of the sensitivity of the QCM sensor on the position of the object relative to the centre of the sensor is considered. Comparison of calculating mass increment and measured values in cases of various fillings and displacement of nanoobjects was carried out.
Effectivity of water hyacinth (Eichhornia crassipes (Mart.) Solms) as bio filter for microbiological water quality in Purwomartani Housing, Kalasan, Sleman, DIY
Authors: Kahol Y V , Ningrum N S , Retnaningrum E , Darmasiwi S
Published: September 2019

Water is one of the most important needs for organisms. However, microbial contamination in water has become serious issue recently. We were interested to reduce the microbial contamination in water by using biological agents such as water hyacinth. In some studies, water hyacinth has antimicrobial activities as well as their function as phytoremediation agent. This research was done to study the effectiveness of water hyacinth (Eichhornia crassipes (Mart.) Solms as bio filter against coliform in water of Mataram water channel in Yogyakarta. The bio filter were arranged as following layer; gravel, zeolite and water hyacinth as the most upper part of bio filter. It was then set with the direction along with the river flow. The water samples were then taken triplicates, each from the upstream (before bio filter treatment) and downstream (after bio filter treatment). It were then continued by Most Probable Number (MPN) test which include the presumptive, confirmed, and completed test. The MPN results showed that coliform in the upstream were found about 57.34 ± 54.35 MPN / 100 ml while in the downstream were about 808 ± 678.96 MPN / 100 ml. The statistical test showed that the difference between upstream and downstream were not significant. Thus it can be concluded that water hyacinth bio filter was not effectively used as bio filter against coliform bacteria.
Screening and molecular identification of Cr(VI)-resistant Trichoderma isolated from ex-tin mining soil in Bangka Belitung Province, Indonesia
Authors: Hastuty A , Hidayat I
Published: September 2019

Several major industries in Indonesia such as the textile industry, electroplating industry, and the pharmaceutical industry, can generate a considerable amount of chromium (Cr) wastes, hexavalent chromium [Cr(VI)]. Unfortunately, the wastewater treatment plant systems of these industries may be partially unqualified, causing heavy pollution in several water bodies such as rivers and lakes. The aim of the present study was to discover and identify Cr(VI)-resistant fungi as bioremediation agents of removing the Cr(VI) from the environment. Seven fungal isolates from tin mining soil of Bangka Belitung province were found tolerant to high Cr(VI) concentration. All fungal isolates grew well at 0.25 mM of Cr(VI), and five fungal isolates still tolerant at a concentration of 2 mM. Molecular identification based on the ITS (internal transcribed spacer) rDNA region showed that these isolates belong to Trichoderma crassum (2 isolates), T. virescentiflavum (3 isolates), and T. aff. tomentosum (2 isolates).
Screening of potential lignin-degrading fungi from the tropical forest for lignocellulose biotreatment
Authors: Idris  , Ramadhani I , Kanti A , Sudiana I M
Published: September 2019

Lignocellulose can be converted into many products replacing products produced from fossil, such as bioenergy, biochemicals and biomaterial products through several treatment. In the bioethanol production, the lignin content in lignocellulose causes the conversion of cellulose to ethanol is less optimal. This research aimed to screen a number of fungal isolated from tropical forests to obtain the lignin-degrading fungi which are potentially used in lignocellulose biotreatment. Screening was conducted by growing fungal isolates on a minimal salt media containing black liquor as a carbon source. Fungal growth ability was observed based on growth index and lignin degradation by fungal isolates was determined by measuring the absorption spectra of the degradation medium at wavelengths of 280 nm and compared to uninoculated control media. The screening results showed that all of 10 isolates had the different ability to grow on minimal media containing black liquor. One fungal isolates identified as Daldania eschscholtzii SA2 146 showed the highest lignin degradation ability that can degrade lignin up to 15% of the total lignin in the media. The lignin degradation by five fungal isolates was range between 5% - 15%. This study proved that the obtained fungal isolates can degrade lignin and potentially be used in lignocellulose biotreatment. Further investigation will be conducted to evaluate the selectivity of potential fungi to degrade lignin.
Enhancement of laccase production in a new isolated Trametes hirsuta LBF-AA017 by lignocellulosic materials and its application for removal of chemical dyes
Authors: Andriani A , Sukorini A , Perwitasari U , Yopi
Published: September 2019

Laccase is one of the extracellular ligninolytic enzymes highly produced by white rot fungi (WRF) and widely used in industrial processes such as decolorizing of synthetic dyes. In this study, the enzyme was produced by a new isolated WRF (Trametes hirsuta LBF-AA017) through two types of fermentation: submerged and solid state fermentation. In the submerged culture, several local lignocellulosic materials (0.5% w/v): bagasse, palm kernel cake (PKC), sugar palm fruit cake (SPFC), corn cobs (CC), tobacco road (TR), were used as laccase inducers. Among 5 materials tested, treatment using SPFC resulted highest laccase production, 645 U L−1 in the 9th day. Higher concentration of SPFC (5% w/v) enhanced laccase poduction by the fungus up to 2034.354 U L−1 in 9 days. Addition of SPFC above 25% (w/v) into culture medium made solid state condition and significantly enhance the production of laccase by the fungus. The crude laccase could effectively decolorize three types of chemical dyes: congo red (CR), brilliant blue G (BBG), coomassie brilliant blue (CBB), in a short period. The laccase could effectively remove three kind of tested dyes with various rates: 45, 91, 48%, respectively for CR, BBG and CBB in one hour reaction. Rapid removal of dyes, especially for BBG, by the laccase T. hirsuta LBF-AA017 has initially proven the potential to be applied for environmental biotechnology.
Decolorization of anthraquinone, azo and triphenylmethane dyes by laccase from newly isolated fungus, Cerrena sp. BMD.TA.1
Authors: Hidayat A , Ningrum A C , Falah S
Published: September 2019

The use of white-rot fungus for dyestuff degradation is alternative of eco-friendly strategy on removal of industrial effluents. This study was conducted to investigate the decolorization of dyes using laccase produced by Cerrena sp. BMD.TA.1 isolated from Gunung Rinjani National Park (GRNP), West Nusa Tenggara – Indonesia. This fungus had capability to decolorize three types of dyes, those: anthraquinone (Remazol Brilliant Blue R, RBBR), azo (Congo Red) and triphenylmethane (Fast Green FCF). The optimum laccase activity in this dyes treatment by fungal culture was achieved at 96 h pre-incubation, but the highest decolorization rate was reached at 144 h pre-incubation. After 72 h of dyes treatment, all dyes were removed at least 82%. The optimum decolorization for 100 mg L−1 was similar for of all dyes types. The decolorization of RRBR was higher compared to Congo Red and Fast Green FCF at 1000 mg L−1. Furthermore in purified laccase, the RBBR was decolorized without any addition of redox mediator. The decreasing of laccase activities and increasing of dye concentration resulted on the lowest decolorization.This study revealed that laccase produced by Cerrena sp. BMD.TA.1 contributed to decolorization process, and had potential industrial application on removal of dyes effluents.
Phytoremediation of chromium (Cr) using Typha angustifolia L., Canna indica L. and Hydrocotyle umbellata L. in surface flow system of constructed wetland
Authors: Taufikurahman T , Pradisa M A S , Amalia S G , Hutahaean G E M
Published: September 2019

Leather tannery industry discharged wastewater containing chromium to the nearby river. The presence of chromium in excess of the limit pollutes the environment and could harm animal and human health. In this study, we examined wetland plants i.e. Typha angustifolia L., Canna indica L. and Hydrocotyle umbellata L. for their effectiveness in remediating chromium in an artificial wetland system within nine days period. Chromium compound, K2Cr2O7, was used with a variation in concentration of 0, 10, 30 and 50 mg/L. All three plants survived until the 9th day. The plant with the highest relative growth was C. indica, whereas plants which showed highest chromium accumulation and bioconcentration factor (BCF) was H. umbellata. Chromium accumulated more in the root than in the shoot (TF <1). Plant with highest efficiency in reducing chromium in medium was T. angustifolia (99.78%) grown in 50 mg/L chromium. The efficiency of C. indica and H. umbellata were 99.67% and 86.36%, respectively. The three plants showed a good potential of phytoremediation for wastewater containing chromium.
Phytoremediation of Maninjau Lake water using Minute Duckweed (Lemna perpusilla Torr.)
Authors: Chrismadha T , Suryono T , Magfiroh M , Mardiati Y , Mulyana E
Published: September 2019

Lake Maninjau is a tecto-volcanic lake in West Sumatra, which is currently facing eutrophication problem and urgently needs measures to be recovered. A preliminary experiment was carried out to assess the possibility of using minute duckweed (Lemna perpusilla Torr.) for healing the lake water, while also producing biomass for alternative feed. This experiment was carried out in The Unit for Technology Transfer of Lake Restoration – Indonesian Institute of Sciences, which located next to Maninjau Lake. It employed 9 plastic containers of 30X20X20 cm3 filled with lake water taken from 3 locations of different level nutrient contamination. As much as 10 g minute duckweed was put into each container and let to grow for 9 days. Observation on the plant growth and biomass productivity along with the water nutrient concentration was carried out every 3 days. At the end of experiment duckweed biomass was sampled for proximate analysis. The results shows that Maninjau Lake water supported only sub-optimal growth of the duckweed, indicated by low growth rate (ranged 8-22 %/day) as well as the biomass protein content (13-15 % DW). The duckweed, however, can eliminate up to 94% phosphate and 54% total in-organic nitrogen from the water, which also indicating that phosphorous was the limiting factor for duckweed growth in the lake.
Bacteriological analysis of Lake Toba
Authors: Melati I , Lukman
Published: September 2019

Lake Toba is designated as a National Tourism destination in North Sumatra. Lake Toba also used as a domestic water source for the surrounding communities, so that studies on water quality especially bacteriological condition need to be carried out. Theaims of this study was to analysis heterotrophic bacteria, E. coli, and total coliform bacteria. In addition, somephysico-chemicalparameters were also studied. Water samples were taken from ten different sampling stations from the eastern, western, northern and southern sides of Lake Toba. Heterotrophic bacteria was analyzed using the spread plate method, the total number count of E.coli and coliform were analyzed using the filtration method.The results showed that physico-chemicalparameters meet the Indonesian water quality criteria of raw materials for drinking water and recreation except the COD. None of samples conformed to Indonesian water standard for sanitation hygiene needs for E. coli densities should not exceed 0 colonies / 100 mL and the total coliform not exceed 50 colonies / 100 mL.The highload of COD, E.coli and total coliform indicated severe contamination of lake with domestic sewage and human excreta. Thus we assumed that water from Lake Toba is not safe for domestic water sources.
Effect of C/N ratio variations on Muara Karang sediment in oxidizing high concentrated ammonia and identification of nitrifying bacteria using VITEK 2
Authors: Ilham S A , Mangunwardoyo W , Ambarsari H
Published: September 2019

Oxidation of ammonia in the environment is known as nitrification. The process itself is a two-step process which oxidizes ammonia into nitrite then nitrate. A research was conducted to study the effect of C/N ratio variations in oxidizing high concentrated ammonia (100 ppm) by the bacteria isolated from the sediments collected from Muara Karang. The variations of C/N ratio implemented in the systems were 5:1, 10:1, 15:1, and 20:1 with glucose as the carbon source and ammonium sulphate as the nitrogen source. Parameters measured during the research were ammonia and nitrate concentrations. Identification of bacteria from the sediments was performed using VITEK 2 identification kits. The results revealed a significant difference (α=0.05) in ammonia concentrations between treated groups, whilst no differences were noted in nitrate (α=0.001) concentrations. Potential nitrification bacteria from the systems were identified as Acinetobacter ursingii (88%), Pseudomonas aeruginosa (99%), and Shewanellaputrefaciens (91%.)
Combined use of Enterobacter cloacae MB20 and the microelements of copper and manganese to control damping-off of tomato
Authors: Yuliar  , Kartadi S F , Salmah A
Published: September 2019

Biocontrol activity of Enterobacter cloacae alone and in combination with copper and mangan on the suppression of tomato damping off was assessed. Rhizoctonia solani damping-off is difficult to control, since its dorman organ sclerotia survive for long periods under various environmental condition. The availability of micronutrients copper and mangan may play important roles in controlling of plant diseases. Results showed that the application of E. cloacae alone decreased damping-off of tomato significantly (P<0.05) by 85%. Combination application of E. clocae with 10μg ml−1 of CuSO4 showed the highest suppression of the disease, followed by the treatment of E. cloacae with 5μg ml−1, as high as 95% and 90% respectively, although the differences was not significant compared to other treatments. Degree of the disease suppression did not differ between application of E. cloacae alone and in combination with both concentration of MnSO4 (μg ml−1 and10μg ml−1), the disease decreased about 85%. Inoculation of R. solani alone into soil resulted significantly highest (P<0.05) of fungal population, it was about 33.7 x 103 CFU g−1 soil, and inoculation of R. solani and E. cloacae reduced significantly fungal population to about 19.0 x103 CFU g−1 soil. E. cloacae produced chitinase and protease, its chitinolytic index was about 0.84, and proteolytic index was 0.61.
Denitrification activity of isolate CD I-III in nitrate broth medium with carbon source variation and identification using VITEK-2
Authors: Auliadita R , Mangunwardoyo W , Ambarsari H
Published: September 2019

Denitrification is a reducing process of nitrate into nitrogen gases. Bacteria are able to do this process in high nitrate and low oxygen concentration. Fresh sediment from Cideng River, Jakarta, was incubated in Nitrate Broth medium then spread on Potato Dextrose Agar. Nine isolates was grown separately and inoculated in Oxidative/Fermentative medium to determine the denitrification ability. One isolate, named Isolate CD I-III, showed a positive result of denitrification activity. This isolate was inoculated in a Nitrate Broth medium with carbon source variations for 19 days to optimize its denitrification process. Parameters measured for indicating the denitrification activity were nitrate concentrations by the Spectrophotometric method and the bacterial viability by the total plate count method. This isolate was also identified by using the VITEK-2. The result revealed that glucose and glycerol as carbon source variations gave a significant difference (α = 0,05) in nitrate concentrations, whilst there was no difference in bacterial viability. The isolate CD I-III was identified by the VITEK-2 as Escherichia hermannii with 97% probability.
The hydrolysis of carbohydrate-based local raw materials as natural sweeteners by extract powder enzyme Aspergillus awamori KT-11 from wheat bran
Authors: Melliawati R , Nuryati  , Mahmudah A F
Published: September 2019

Aspergillus awamori KT-11 is a proven potential mold produces the amylase enzyme complex, particularly amylase and glucosidase. These enzymes hydrolyze the starch being sugar. Food and beverage industry began extensive use of liquid sugar because it has several advantages. Liquid sugar is raw materials of starch in nature Indonesia. This purpose of this research is to extract powder enzyme Aspergillus awamori KT-11 from wheat bran against 4 types of raw material-based local carbohydrate to produce natural sweetener (liquid sugar).The raw material used namely potato, breadfruit, yellow squash, and bananas. Enzyme activity produced from mold Aspergillus awamori KT-11 on wheat bran of 186,9835 U/ml, reducing sugar of 9.775 mg/ml and protein levels is 48.981 mg/ml. The sweetener from natural potato starch, breadfruit, yellow squash, and banana produces different percentages. The highest percentage of banana flour produced at 117.938 mg/ml (concentration of flour 20%, enzyme 15% and incubation time for 3 days ) and lowest on yellow squash (6.563 mg/ml). The result shows that TLC from potato flour, breadfruit and bananas are produced of glucose and lactose, is from flour yellow squash generated fructose.
Expression of gene encoding raw starch digesting α -amylase (Amyl III) in Escherichia coli
Authors: Anindyawati T
Published: September 2019

Amylase generally known as an industrial enzyme that hydrolyzes starch and glycogen. The α-amylase hydrolyzes α-1,4 glycosidic linkage that acts on raw starch. This reaction is very important for direct starch processing for alcohol fermentation and functional oligosaccharide or food additives development. Amyl III gene was isolated from Aspergillus awamori KT-11 and inserted into pDONR 201 as entry clone. The recombinant construct was subcloned into pDEST 17 plasmid as the expression vector and into E. coli BL21AI cells. The objective of this study was to optimize induction temperatures in the expression process and to know the ability of recombinant Amyl III to hydrolyze raw starch. The expression study of Amyl III was performed using LB medium at 27°C and 37°C. Purification was done using affinity chromatography (His Tag) with 100 mM imidazole. Protein analysis was done using 7.5% of SDS-PAGE gel. Digestion on soluble starch and raw waxy maize starch by Amyl III was performed using TLC plate. The results show that the best protein expression was obtained by incubation at 27°C. Digestion on those starch by using recombinant Amyl III resulted in the production of malto-tetraose, -pentaose, -hexaose and -heptaose.
Potential plant as a natural dyes in Toba Lake, North Sumatra
Authors: Djarwaningsih T
Published: September 2019

Ulos is a woven fabric widely used by Batak tribe in North Sumatra., which is a source of warmth both and physically. In ancient times to complete the work of ulos woven fabrics, craftsmen use yarn with natural dye whose source comes from plant, shrubs and other ingredients. Natural dyes are very important in the textile industry, especially the more pro-environment and prevent skin allergies for the consumer. Natural dyes are non-toxic, easy decompose, environmentally friendly, renewable. The rise of ulos fabric manufacturers with chemical dyes in some areas around Lake Toba, North Sumatra, has increasingly marginalized the original ulos craftsmen, who still maintain natural dyes. The research was conducted by inventory at selected locations, interview and detection of natural plant dyes. The purpose of this research is to obtain new information, data related to natural dyes, and the addition of information other ingredients which potential as a natural dye especially of ulos in Toba lake area, North Sumatra. Based on the observation results in several locations in Toba lake area, has been found 18 plants species and four other ingredients that have the potential as natural dyes of ulos.
Screening of antimicrobial and toxicity activity of endophytic bacteria associated with Curcuma Zedoaria
Authors: Sulistiyani T R , Lisdiyanti P , Lestari Y
Published: September 2019

Indonesian has been usedCurcuma zedoariaas a herbal in cancer treatment. The bioactive compounds contained in the medicinal plants are products by the plant itself or by endophytes living inside the plant. Zebrafish has been used for the study in several areas of cancer research, including angiogenesis, metastasis, antitumor drug screening, and toxicity. This study was to investigate the antimicrobial and toxicity activity of secondary metabolite from endophytic bacteria isolated fromC. zedoaria. Endophytic isolates were screened for antimicrobial activities against six microbial pathogensBacillus subtilis, Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, Candida albicans, andSacharomyces cerevisiae. Four of endophytic bacteria showed antimicrobial activity were tested in toxic compound producing through Fish Embryo Toxicity (FET) test using zebrafish. The results indicated that from 73 selected isolates, 16 isolates (21.92%) showed antimicrobial activities against at least one of the test organisms. Four of endophytic bacteria that showed high antimicrobial activities, were identified asCitrobacter freundii, Bacillus subtilis, Pseudomonas otitidis, andBurkholderia cenocepacia. FET test of ethyl acetate extracts from the four those selected isolates revealed that they had LC50values 63.5; 24.7; 13.4; and 12.6 ppm, respectively. These result showed that the highest toxicity was obtained fromB. cenocepaciaextract.
Antihypertension Activity of Cymbopogon nardus (L.) Rendle as an Aromatherapy Candle Material at Community Health Center Kapuk II West Jakarta, Indonesia
Authors: Yelfi A , Napitupulu T P , Meliana D U
Published: September 2019

The utilization of natural materials is likely to increase nowadays, including for aromatherapy purposes. In this research, aromatherapy candle had been made from the volatile oil of Cymbopogon nardus (L.) Rendle (citronella grass). The aims of this research is to observe the influence of aromatherapy candle containing citronella grass oil toward the blood pressure of hypertension patients at Community Health Center Kapuk II Jakarta, Indonesia. This research used pre-experiment design with one group pretest-posttest design. The population was elderly who had high blood pressure. A sample of 43 peoples were obtained by simple random sampling technique. Data analysis was done by t-dependent test (paired t-Test) with significance value α = 0,05. The result of paired t-Test from pretest and posttest of systole blood pressure was obtained with P value 0,000 and paired t-test from pretest and posttest of diastolic blood pressure was obtained with P value 0,012. It can be concluded that there was an influence of aromatherapy candles of Cymbopogon nardus (L) Rendle toward lowering blood pressure in hypertension patient at Puskesmas Kapuk II Jakarta Indonesia.
The role of indigenous Rhizobia on Paraserianthes falcataria (L) Nielsen seedlings in nickel post mining lands
Authors: Sari R , Prayudyaningsih R
Published: September 2019

Application of indigenous Rhizobia on degraded land reclamation, especially post mining land allegedly can promote plant growth and development.. The research was to determine effectiveness of indigenous Rhizobia isolates from nickel post mining land to the growth of P. falcataria (L) Nielsen seedlings. Three types of native Rhizobia isolates (R1, R2, R3) and their combination (R4, R5, R6, R7) were applied as treatments in randomized completely design. All data were analyzed using Analysis of Variance (ANOVA) and Kruskal Walis. Significant differences between treatments were analyzed using Duncan’s Multiple Range Test (DMRT) and Mann Whitney. The result showed most of growth variables of seedlings was not significantly influenced by Rhizobia inoculation, except stem diameter and biomass. This research also indicated correlation between N content in plant tissue with number of nodules, diameter and biomass although not significant. Single inoculation of Rhizobia did not show optimal results on P. falcataria (L) Nielsen seedlings so double inoculation using other potential microbes or input organic fertilizers at certain levels allegedly can increase the potential of Rhizobia on plants, especially in nickel post mining reclamation.
Production of indole acetic acid by Enterobacter cloacea H3 isolated from Mungbean (Vigna radiata) and its potential supporting the growth of soybean seedling
Authors: Widowati T , Nuriyanah  , Sukiman H
Published: September 2019

Many plant-associated bacteria synthesize the phytohormone indole acetic acid (IAA). Three bacterial isolates (H1, H2 and H3) were isolated from root nodules of Mungbean (Vigna radiata) and tested for their productivity of indole acetic acid by spectrophotometric method. Isolate H3 showed the highest IAA production (12.28 μg/ml) in culture medium supplemented with L-Tryptophan. Based on 16S rRNA gene analysis, isolate H3 was identified as Enterobacter cloacae. The cultures condition optimized for maximum IAA production by using different pH and tryptophan concentrations. IAA production of isolate H3 was maximum at pH 6 and 5 mg/ml of tryptophan concentration. The highest IAA producing isolate selected for determining its capability and compatibility to support the germination of soybean. The results showed that germination percentage and rate of soybean were not significantly different between control and isolate H3 treatment.
Study of cross inoculation of Rhizobium tropici with other potential soil microbes on their ability to support the growth of Soybean
Authors: Lekatompessy S , Nurjanah L , Sukiman H
Published: September 2019

Rhizobia bacteria have been well known as nitrogen-fixing bacteria which could promote the growth of soybean plants. The bacteria could establish a symbiosis living with crops and trees legume. The successful symbiosis living is normally identified by the specific compatibilities between the bacteria and host plants. However, there is a chance that the bacteria which was isolated from trees legumes could also infect and be compatible in developing the symbiosis living with crops plant such as soybean. This study aims to confirm that Rhizobium bacteria isolated from tree legume, Acacia mangium, could successfully develop the symbiosis living with soybean. In addition, this research also studies the possibility of Rhizobium bacteria in working with other potential soil microbes such as mycorrhizae and others potential microbes on supporting the growth of soybean. Green house experiment showed that Rhizobium tropici combined with other potential soil microbes could significantly support the growth of soybean. This is shown with the increase of upper plant biomass. A number of pods although the data of visible soybean seed did not make significant different. This research finally opens the possibility of using biodiversity of nitrogen-fixing microbes to be packed as an update quality biofertilizer for soybean.
Potency of nitrogen fixing bacteria isolated from POME disposal pond and their effect on the growth of Caesalpinia pulcherrima (L) Sw
Authors: Widawati S , Suliasih
Published: September 2019

Nitrogen fixing bacteria (NFB) play an important role to promote plant growth through nitrogen fixation and IAA hormone production. The objective of this research was to obtain the functional bacteria (nitrogen fixing bacteria) isolated from POME disposal pond that show the ability in nitrogen fixation and the potential as plant growth promoting rhizobacteria (PGPR), as well as to investigate the potential of these bacteria through in vitro and in vivo growth of Caesalpinia pulcherrima (L) Sw, and its effect on bacterial populations in media contaminated by POME. In vitro plant growth promoting activity characterization included N-fixing activity, Indole Acetic Acid production, and C. pulcherrima germination (inoculant + POME). In vivo plant growth promoting activity used sterile sand inoculated with NFB and watered with POME, whereas the control was not inoculated and watered with aquadest. Five strains were found as potential PGPR, namely Azotobacter sp., Azospirillum sp1-3., and Rhizobium sp. In addition, Azospirillum sp.1 is the best PGPR that produced highest seed vigour index (SVI), seed germination in vitro, and seed germination in vivo respectively. Azospirillum sp. 1 and Rhizobium sp. have the ability to promote growth of C. pulcherrima in vitro and in vivo with bacterial populations as much as 107cfu mL−1 in POME contaminated media. The bacteria and C. pulcherrima are potential for reclaiming infertile soil.
Ecological and physiological characterization of ACC deaminase-producing bacteria isolated from specific sites in Yogyakarta, Indonesia
Authors: Simarmata R , Salamah U , Ngadiman  , Rohman S , Simanjuntak P
Published: September 2019

The decrease of plant growth and productivity may be due to environmental stresses that induce excessive ethylene synthesis. ACC (1-aminocyclopropane-1-carboxylate) deaminase-producing bacteria are known to decrease plant ethylene levels by converting the ethylene precursor, ACC to ammonia and α-ketobutyrate. This study was aimed to understanding ecological and physiological characters of ACC deaminase-producing bacteria. The bacterial strains used were Sphingobacterium multivorum BK1, Bacillus mycoides CB2, Pantoea dispersa CK4, Pantoea agglomerans KD6.2, Enterobacter ludwigii KW3, Bacillus aryabhattai TW7, Pseudomonas monteilii KS12, Pseudomonas putida KS16.2, Pseudomonas putida PIR 3C, Stenotrophomonas maltophilia PIR5, Lysinibacillus pakistanensis PIC5, Bacillus aryabhattai PIC11, and Roultella terrigena PCM8. It was observed that all bacterial strains demonstrated optimal growth at pH 6-8, temperature 25–30°C, and oxygen requirement of aerobic to facultative anaerobic. All of strains have Phosphate Solubilizing Index between 1,1–3,04 and IAA (Indole-3-Acetic Acid) production level between 0,11-10,33 ppm.hour-1. In terms of antagonistic properties, all bacterial strains were found incapable of inhibiting Xanthomonas oryzae pv. oryzae and Ralstonia solanacearum growth. All bacterial strains were capable of using glucose, sucrose, cellulose and mannitol as the source of carbon and energy. In addition, all bacterial strains also demonstrated Cr6+ tolerance ofs 100–300 mg.L-1.
Sengon (Falcataria moluccana) prevention from gall rust through seed irradiation: field test in endemic location
Authors: Yulianti  , Putri K P , Nugraheni Y M M A , Danu
Published: September 2019

The gall rust disease is the diseases of sengon plant (Falcataria moluccana), that it caused by Uromycladium spp. which can cause the decreased productivity. However, at this time not yet retrieved the right techniques to reduce attacks of gall rust disease on sengon plant. Efforts to control gall rust disease at the level of seeds can be through by seed irradiation technique. Irradiation techniques on seeds can be used to increase productivity through increased vigour and seed quality. The aim of the study was to determine the effectiveness of gamma rays irradiation techniques on sengon seeds its shown by the growth and percentage of damage caused gall rust at sengon plantation at Pandantoyo Sub Forest District (RPH), Pare Forest District (BKPH), Kediri Forest Management Unit (KPH), East Java. The research design used a randomized block design consisting of 3 blocks. Each block consists of 10 treatments based on irradiated doses (0, 5, 10, 15, 30, 45, 60, 75, 90 and 105 Gy) and each treatment consist of 20 plants. The parameters observed were percentage of attacked plants that divided into four level of damage: healthy (0%); low (≤ 10%), moderate (≥ 11%-45%) and severe (≥ 46%) and growth of diameter and height. Generally, the health conditions of sengon plantation were affected by gall rust. At 18-months-old, the gamma rays irradiation at 75 Gy was the largest percentage of healthy plants (42.5%) and better than control (24 %). At 25-months-old, the plants derived from seeds that were not irradiated (control) was the largest percentage of plants with severe damage. Gamma rays irradiation does not affect the growth of diameter and height. The highest percentage of healthy plants were at 1st block but the fastest growth of plant diameter was 2nd block.
Development of UAV technology in seed dropping for aerial revegetation practices in Indonesia
Authors: Andrio A
Published: September 2019

The Unmanned Aerial Vehicles (UAVs) applications or known as quadcopter drones have been considerably growing since the 20th century through now. In this project, we introduce the use of drone to support the revegetation practices. The project aims to develop a quadcopter drone that is capable of dispersing Tamarindus indica seeds in revegetation fields with potential for revegetation. T. indica is tree species that commonly used for revegetation in Indonesia. This work includes following steps. First, a drone takes an aerial photo of the demonstration plots to highlight existing vegetation and obstruction. The result is the information that is used to develop the best seed dropping and planting pattern. The map then is uploaded into the drone. Second step is developing mechanized canisters containing a germinated seed. The seeds then released from canisters installed in drone and break open upon impact with the soil to allow the germinated seeds to take root in the predetermined positions. The proposed drone assisted seed dropping system will provide an essential tool for an efficient revegetation. Due to the effectiveness, this system is also suitable for revegetation in ecosystem where germination has not occurred or for planting in areas with difficult access.
Soil characteristics of rattan agroforests in Katingan district, Central Kalimantan
Authors: Tata H L
Published: September 2019

Rattan agroforest is a swidden cultivation systems, which apply slash-burning and fallow rotation in its development. This system is commonly practised by local farmers of Dayak in Central Kalimantan. The objective of the study was to assess soil characteristics of 5 land use types, namely forest, secondary forest (baliang), young rattan agroforest (bahu), complex ruber-rattan agroforest (CRR), and simple rubber agroforest (SR) in two villages in Katingan. The method used in the study was regular methods of soil analysis, such as pH (H2O &KCl), Corganic(Walkley & Black), Pavailable (Bray), Ntotal (Kjeldahl), and soil texture. The result showed that in the top layer (0-10 cm depth), forest has the highest C/N ratio, but the lowest ratio of Corg/Cref compare to others. Forest has the lowest Pcontent (4.02 ppm), while SR has the highest Pcontent (12.06 ppm). CRR and SR tends to have higher clay content than sand content. In general, farming systems determine characteristics of the soils. Forest conserves soil characteristics, while management that applied by farmers in the agroforestry system may improve soil fertility.
Natural revegetation of tailing deposited on agricultural area in Buru, Maluku
Authors: Hindersah R , Kalay A M , Komarya A , Kamaluddin N N
Published: September 2019

Tailing deposited on rice field in Burn District Indonesia poses a severe environment problem since rice does not grow on tailing with low fertility. The aim of this study was to verify the natural vegetation grown naturally on mercury contaminated tailing which covered the top soil of rice field, as well as the development of legume tree, Trembesi, Rain Tree (Albizia saman F. Muell) grown in purpose between the pioneer plants. Plant succession observations were carried out for 2,5 years on 524 m2 of rice fields. As presumed, the succession was similar to the general pattern of primary revegetation. The study area increased diversity of weed as primary vegetation which influenced the better growth of Trembesi. A total of 17 natural plants were recorded as pioneer weed as well as late colonizer. Weed grazing by livestock, mainly cow, promoted the diversity of plants due to their role as seed dispersal agents.
Evaluation of mangrove stand planted for rehabilitation using guludan technique in coastal area of Angke Kapuk, Jakarta
Authors: Ashari R , Kusmana C , Kuncahyo B
Published: September 2019

Guludan technique becomes an alternative for mangrove rehabilitation in the disused fishponds. This technique has been implemented since 2005 on the coast of Jakarta. The wider application of this technique requires several studies in advance to see growth of the mangrove in the already planted guludan. This study aims to measure the growth of mangroves planted by guludan techniques, identify environmental factors that affect their mangrove growth, and analyze their relationship. Measurements were performed on 40 guludan samples which planted by Rhizopora mucronata in 2010 - 2013. R. mucronata can grew in guludans with highest growth percentage in the 2013 group of 56.53%. The 2010 planting group showed highest average height, average diameter and basal average area of 4.59 m; 3.91 cm and; 28.87 m2/ha respectively. S. Caseolaris grew naturally in guludans and become competitor of R. mucronata in obtaining space to grow. The 2012 group was the highest in terms of S. caseolaris density, that is 15 individuals/guludan with average height, average diameter and basal area of 9.38 cm, 5.29 m, and 24.79 m2/ha, respectively. The growth of R. mucronatawas affected by nutrients (N, P, Ca), clay texture, and other soil factors (CEC and pH), whereas S. caseolaris was only affected by nutrients (N, P, and Ca).