bluuebunny/crossref_metadata_2025 huggingface dataset:
High Level of Staufen1 Expression Confers Longer Recurrence Free Survival to Non-Small Cell Lung Cancer Patients by Promoting THBS1 mRNA Degradation
Authors: Bonnet-Magnaval Florence , Diallo Leïla Halidou , Brunchault Valérie , Laugero Nathalie , Morfoisse Florent , David Florian , Roussel Emilie , Nougue Manon , Zamora Audrey , Marchaud Emmanuelle , Tatin Florence , Prats Anne-Catherine , Garmy-Susini Barbara , DesGroseillers Luc , Lacazette Eric
Published: December 2021

Stau1 is a pluripotent RNA-binding protein that is responsible for the post-transcriptional regulation of a multitude of transcripts. Here, we observed that lung cancer patients with a high Stau1 expression have a longer recurrence free survival. Strikingly, Stau1 did not impair cell proliferation in vitro, but rather cell migration and cell adhesion. In vivo, Stau1 depletion favored tumor progression and metastases development. In addition, Stau1 depletion strongly impaired vessel maturation. Among a panel of candidate genes, we specifically identified the mRNA encoding the cell adhesion molecule Thrombospondin 1 (THBS1) as a new target for Staufen-mediated mRNA decay. Altogether, our results suggest that regulation of THBS1 expression by Stau1 may be a key process involved in lung cancer progression.
A Multi-Omics Network of a Seven-Gene Prognostic Signature for Non-Small Cell Lung Cancer
Authors: Ye Qing , Falatovich Brianne , Singh Salvi , Ivanov Alexey V. , Eubank Timothy D. , Guo Nancy Lan
Published: December 2021

There is an unmet clinical need to identify patients with early-stage non-small cell lung cancer (NSCLC) who are likely to develop recurrence and to predict their therapeutic responses. Our previous study developed a qRT-PCR-based seven-gene microfluidic assay to predict the recurrence risk and the clinical benefits of chemotherapy. This study showed it was feasible to apply this seven-gene panel in RNA sequencing profiles of The Cancer Genome Atlas (TCGA) NSCLC patients (n = 923) in randomly partitioned feasibility-training and validation sets (p < 0.05, Kaplan–Meier analysis). Using Boolean implication networks, DNA copy number variation-mediated transcriptional regulatory network of the seven-gene signature was identified in multiple NSCLC cohorts (n = 371). The multi-omics network genes, including PD-L1, were significantly correlated with immune infiltration and drug response to 10 commonly used drugs for treating NSCLC. ZNF71 protein expression was positively correlated with epithelial markers and was negatively correlated with mesenchymal markers in NSCLC cell lines in Western blots. PI3K was identified as a relevant pathway of proliferation networks involving ZNF71 and its isoforms formulated with CRISPR-Cas9 and RNA interference (RNAi) profiles. Based on the gene expression of the multi-omics network, repositioning drugs were identified for NSCLC treatment.
Manganese Is a Strong Specific Activator of the RNA Synthetic Activity of Human Polη
Authors: Balint Eva , Unk Ildiko
Published: December 2021

DNA polymerase η (Polη) is a translesion synthesis polymerase that can bypass different DNA lesions with varying efficiency and fidelity. Its most well-known function is the error-free bypass of ultraviolet light-induced cyclobutane pyrimidine dimers. The lack of this unique ability in humans leads to the development of a cancer-predisposing disease, the variant form of xeroderma pigmentosum. Human Polη can insert rNTPs during DNA synthesis, though with much lower efficiency than dNTPs, and it can even extend an RNA chain with ribonucleotides. We have previously shown that Mn2+ is a specific activator of the RNA synthetic activity of yeast Polη that increases the efficiency of the reaction by several thousand-fold over Mg2+. In this study, our goal was to investigate the metal cofactor dependence of RNA synthesis by human Polη. We found that out of the investigated metal cations, only Mn2+ supported robust RNA synthesis. Steady state kinetic analysis showed that Mn2+ activated the reaction a thousand-fold compared to Mg2+, even during DNA damage bypass opposite 8-oxoG and TT dimer. Our results revealed a two order of magnitude higher affinity of human Polη towards ribonucleotides in the presence of Mn2+ compared to Mg2+. It is noteworthy that activation occurred without lowering the base selectivity of the enzyme on undamaged templates, whereas the fidelity decreased across a TT dimer. In summary, our data strongly suggest that, like with its yeast homolog, Mn2+ is the proper metal cofactor of hPolη during RNA chain extension, and selective metal cofactor utilization contributes to switching between its DNA and RNA synthetic activities.
Green Efficient One-Pot Synthesis and Separation of Nitrones in Water Assisted by a Self-Assembled Nanoreactor
Authors: Patamia Vincenzo , Floresta Giuseppe , Pistarà Venerando , Rescifina Antonio
Published: December 2021

This article reports an alternative method for preparing nitrones using a tetrahedral capsule as a nanoreactor in water. Using the hydrophobic cavity of the capsule allowed us to reduce the reaction times and easily separate the nitrones from the reaction mixture, obtaining reaction yields equal or comparable to those obtained with the methods already reported. Furthermore, at the basis of this methodology, there is an eco-friendly approach carried out that can certainly be extended to other synthesis methods for the preparation of other substrates by exploiting various types of macrocyclic hosts, suitably designed and widely used in supramolecular chemistry.
Which Parameter Influences Local Disease-Free Survival after Radiation Therapy Due to Osteolytic Metastasis? A Retrospective Study with Pre- and Post-Radiation Therapy MRI including Diffusion-Weighted Images
Authors: Lee Jiyeong , Yoon Young Cheol , Lee Ji Hyun , Kim Hyun Su
Published: December 2021

Although radiation therapy (RT) plays an important role in the palliation of localized bone metastases, there is no consensus on a reliable method for evaluating treatment response. Therefore, we retrospectively evaluated the potential of magnetic resonance imaging (MRI) using apparent diffusion coefficient (ADC) maps and conventional images in whole-tumor volumetric analysis of texture features for assessing treatment response after RT. For this purpose, 28 patients who received RT for osteolytic bone metastasis and underwent both pre- and post-RT MRI were enrolled. Volumetric ADC histograms and conventional parameters were compared. Cox regression analyses were used to determine whether the change ratio in these parameters was associated with local disease progression-free survival (LDPFS). The ADCmaximum, ADCmean, ADCmedian, ADCSD, maximum diameter, and volume of the target lesions after RT significantly increased. Change ratios of ADCmean < 1.41, tumor diameter ≥ 1.17, and tumor volume ≥ 1.55 were significant predictors of poor LDPFS. Whole-tumor volumetric ADC analysis might be utilized for monitoring patient response to RT and potentially useful in predicting clinical outcomes.
Novel Reactive Flex Configuration in Kiwi Wing Foil Surfboard
Authors: Fat Cheung Adrien M. , Bari Klaudio
Published: December 2021

The creation of an ideal surfboard is art. The design and construction depend on the individual surfer’s skill level and type of the required performance. In this research, four fuselage concepts were carefully explored to meet the following unique needs: lightweight, strong, and a fast-manufacturing process. The fuselages were manufactured by compression moulding using skin and core materials. The skin material was selected to be unidirectional (UD) carbon fibre, discontinuous carbon fibre (SMC) and Filava quadriaxial fibre impregnated with epoxy, while the core material was selected to be lightweight PVC foam. To assess the mechanical performance, three-point bending has been performed according to BS-ISO 14125 and validated using Finite Element Analysis (FEA) using Ansys software. As expected, the flexural test revealed that the UD carbon fibre fuselage was the strongest and SMC was the weakest, while large deflection was seen in Filava fibre fuselages before failure, showing great reactive flex that promotes projection during surfing. The experimental results show good agreement with FEA simulation, and the locations of the physical failure in the fuselage matches the location of maximum flexural stress obtained from FEA simulation. Although all fuselages were found to carry a surfer weight of 150 kg, including a factor of safety 3, except the SMC fuselage, due to shrinkage. The Filava fibre fuselages were seen to have a large deflection before failure, showing great flexibility to handle high ocean waves. This promotes the potential use of reactive flex in high performance sports equipment, such as surfing boards. A large shrinkage must be taken under consideration during compression moulding that depends on fibre orientation, resin nature, and part geometry.
Identification of Pneumocystis jirovecii with Fluorescence In-Situ Hybridization (FISH) in Patient Samples—A Proof-of-Principle
Authors: de Sousa Débora Raysa Teixeira , da Silva Neto João Ricardo , da Silva Roberto Moreira , Cruz Kátia Santana , Poppert Sven , Frickmann Hagen , Souza João Vicente Braga
Published: December 2021

In resource-limited settings, where pneumocystosis in immunocompromised patients is infrequently observed, cost-efficient, reliable, and sensitive approaches for the diagnostic identification of Pneumocystis jirovecii in human tissue samples are desirable. Here, an in-house fluorescence in situ hybridization assay was comparatively evaluated against Grocott’s staining as a reference standard with 30 paraffin-embedded tissue samples as well as against in-house real-time PCR with 30 respiratory secretions from immunocompromised patients with clinical suspicion of pneumocystosis. All pneumocystosis patients included in the study suffered from HIV/AIDS. Compared with Grocott’s staining as the reference standard, sensitivity of the FISH assay was 100% (13/13), specificity was 41% (7/17), and the overall concordance was 66.7% with tissue samples. With respiratory specimens, sensitivity was 83.3% (10/12), specificity was 100% (18/18), and the overall concordance was 93.3% as compared with real-time PCR. It remained unresolved to which proportions sensitivity limitations of Grocott’s staining or autofluorescence phenomena affecting the FISH assay accounted for the recorded reduced specificity with the tissue samples. The assessment confirmed Pneumocystis FISH in lung tissue as a highly sensitive screening approach; however, dissatisfying specificity in paraffin-embedded biopsies calls for confirmatory testing with other techniques in case of positive FISH screening results. In respiratory secretions, acceptable sensitivity and excellent specificity were demonstrated for the diagnostic application of the P. jirovecii-specific FISH assay.
Nonwearable Sensor-Based In-Home Assessment of Subtle Daily Behavioral Changes as a Candidate Biomarker for Mild Cognitive Impairment
Authors: Yamasaki Takao , Kumagai Shuzo
Published: December 2021

Patients show subtle changes in daily behavioral patterns, revealed by traditional assessments (e.g., performance- or questionnaire-based assessments) even in the early stage of Alzheimer’s disease (AD; i.e., the mild cognitive impairment (MCI) stage). An increase in studies on the assessment of daily behavioral changes in patients with MCI and AD using digital technologies (e.g., wearable and nonwearable sensor-based assessment) has been noted in recent years. In addition, more objective, quantitative, and realistic evidence of altered daily behavioral patterns in patients with MCI and AD has been provided by digital technologies rather than traditional assessments. Therefore, this study hypothesized that the assessment of daily behavioral changes with digital technologies can replace or assist traditional assessment methods for early MCI and AD detection. In this review, we focused on research using nonwearable sensor-based in-home assessment. Previous studies on the assessment of behavioral changes in MCI and AD using traditional performance- or questionnaire-based assessments are first described. Next, an overview of previous studies on the assessment of behavioral changes in MCI and AD using nonwearable sensor-based in-home assessment is provided. Finally, the usefulness and problems of nonwearable sensor-based in-home assessment for early MCI and AD detection are discussed. In conclusion, this review stresses that subtle changes in daily behavioral patterns detected by nonwearable sensor-based in-home assessment can be early MCI and AD biomarkers.
Recent Trends in Computational Biomedical Research
Authors: Altaf-Ul-Amin Md. , Kanaya Shigehiko , Ono Naoaki , Huang Ming
Published: December 2021

Recent advances in information technology have brought forth a paradigm shift in science, especially in the biology and medical fields [...]
Third-Generation Sequencing: The Spearhead towards the Radical Transformation of Modern Genomics
Authors: Athanasopoulou Konstantina , Boti Michaela A. , Adamopoulos Panagiotis G. , Skourou Paraskevi C. , Scorilas Andreas
Published: December 2021

Although next-generation sequencing (NGS) technology revolutionized sequencing, offering a tremendous sequencing capacity with groundbreaking depth and accuracy, it continues to demonstrate serious limitations. In the early 2010s, the introduction of a novel set of sequencing methodologies, presented by two platforms, Pacific Biosciences (PacBio) and Oxford Nanopore Sequencing (ONT), gave birth to third-generation sequencing (TGS). The innovative long-read technologies turn genome sequencing into an ease-of-handle procedure by greatly reducing the average time of library construction workflows and simplifying the process of de novo genome assembly due to the generation of long reads. Long sequencing reads produced by both TGS methodologies have already facilitated the decipherment of transcriptional profiling since they enable the identification of full-length transcripts without the need for assembly or the use of sophisticated bioinformatics tools. Long-read technologies have also provided new insights into the field of epitranscriptomics, by allowing the direct detection of RNA modifications on native RNA molecules. This review highlights the advantageous features of the newly introduced TGS technologies, discusses their limitations and provides an in-depth comparison regarding their scientific background and available protocols as well as their potential utility in research and clinical applications.
Viscoelastic Polyurethane Foams for Use as Auxiliary Materials in Orthopedics
Authors: Grzęda Dominik , Węgrzyk Grzegorz , Leszczyńska Milena , Szczepkowski Leonard , Gloc Michał , Ryszkowska Joanna
Published: December 2021

One of the essential factors in prostheses is their fitting. To assemble a prosthesis with the residual limb, so-called liners are used. Liners used currently are criticized by users for their lack of comfort, causing excessive sweating and skin irritation. The objective of the work was to develop viscoelastic polyurethane foams for use in limb prostheses. As part of the work, foams were produced with different isocyanate indexes (0.6–0.9) and water content (1, 2 and 3 php). The produced foams were characterized by scanning electron microscopy, computer microtomography, infrared spectroscopy, thermogravimetry and differential scanning calorimetry. Measurements also included apparent density, recovery time, rebound elasticity, permanent deformation, compressive stress value and sweat absorption. The results were discussed in the context of modifying the foam recipe. The performance properties of the foams, such as recovery time, hardness, resilience and sweat absorption, indicate that foams that will be suitable for prosthetic applications are foams with a water content of 2 php produced with an isocyanate index of 0.8 and 0.9.
Prediction of Bending, Buckling and Free-Vibration Behaviors of 3D Textile Composite Plates by Using VAM-Based Equivalent Model
Authors: Xi Senbiao , Zhong Yifeng , Shi Zheng , Yi Qingshan
Published: December 2021

To solve the microstructure-related complexity of a three-dimensional textile composite, a novel equivalent model was established based on the variational asymptotic method. The constitutive modeling of 3D unit cell within the plate was performed to obtain the equivalent stiffness, which can be inputted into the 2D equivalent model (2D-EPM) to perform the bending, free-vibration and buckling analysis. The correctness and effectiveness of the 2D-EPM was validated by comparing with the results from 3D FE model (3D-FEM) under various conditions. The influence of yarn width and spacing on the equivalent stiffness was also discussed. Finally, the effective performances of 3D textile composite plate and 2D plain-woven laminate with the same thickness and yarn content were compared. The results revealed that the bending, buckling and free-vibration behaviors predicted by 2D-EPM were in good agreement with 3D-FEM, and the local field distributions within the unit cell of 3D textile composite plate were well captured. Compared with the 2D plain-woven laminate, the displacement of 3D textile composite plate was relatively larger under the uniform load, which may due to the fact that the through-the-thickness constrains of the former are only dependent on the binder yarns, while the warp yarns and weft yarns of the latter are intertwined closely.
Effect of the Processing Parameters on the Porosity and Mechanical Behavior of Titanium Samples with Bimodal Microstructure Produced via Hot Pressing
Authors: Chávez-Vásconez Ricardo , Lascano Sheila , Sauceda Sergio , Reyes-Valenzuela Mauricio , Salvo Christopher , Mangalaraja Ramalinga Viswanathan , Gotor Francisco José , Arévalo Cristina , Torres Yadir
Published: December 2021

Commercially pure (c.p.) titanium grade IV with a bimodal microstructure is a promising material for biomedical implants. The influence of the processing parameters on the physical, microstructural, and mechanical properties was investigated. The bimodal microstructure was achieved from the blends of powder particles with different sizes, while the porous structure was obtained using the space-holder technique (50 vol.% of ammonium bicarbonate). Mechanically milled powders (10 and 20 h) were mixed in 50 wt.% or 75 wt.% with c.p. titanium. Four different mixtures of powders were precompacted via uniaxial cold pressing at 400 MPa. Then, the specimens were sintered at 750 °C via hot pressing in an argon gas atmosphere. The presence of a bimodal microstructure, comprised of small-grain regions separated by coarse-grain ones, was confirmed by optical and scanning electron microscopies. The samples with a bimodal microstructure exhibited an increase in the porosity compared with the commercially available pure Ti. In addition, the hardness was increased while the Young’s modulus was decreased in the specimens with 75 wt.% of the milled powders (20 h).
Reactive Oxygen Species-Sensitive Nanophotosensitizers of Methoxy Poly(ethylene glycol)-Chlorin e6/Phenyl Boronic Acid Pinacol Ester Conjugates Having Diselenide Linkages for Photodynamic Therapy of Cervical Cancer Cells
Authors: Yang Ju-Il , Lee Hye-Lim , Choi Seon-Hee , Kim Jungsoo , Yu Young-Bob , Jeong Young-IL , Kang Dae-Hwan
Published: December 2021

The aim of this study is to fabricate nanophotosensitizers composed of methoxy poly(ethylene glycol) (mPEG), chlorin e6 (Ce6), and phenylboronic acid pinacol ester (PBAP) with diselenide linkages for reactive oxygen species (ROS)-sensitive photodynamic therapy (PDT) of cervical cancer cells. To fabricate nanophotosensitizers, Ce6 was conjugated with mPEG via selenocystamine linkage and then remaining carboxylic acid groups of Ce6 was attached to PBAP (mPEGseseCe6PBAP conjugates). Nanophotosensitizers of mPEGseseCe6PBAP conjugates were prepared by dialysis method. In transmission electron microscope (TEM) observation, nanophotosensitizers of mPEGseseCe6PBAP conjugates have spherical shapes and their diameters were less than 150 nm. The average diameter of mPEGseseCe6PBAP nanophotosensitizers was 92.7 ± 9.6 nm in particle size analysis. When H2O2 was added to the nanophotosensitizer solution, nanophotosensitizers were sensitively disintegrated according to the H2O2 concentration and then changed from monomodal distribution to multimodal distribution in particle size distribution. Furthermore, Ce6 release from nanophotosensitizers also increased according to the H2O2 concentration. When H2O2 was added to cell culture of HeLa human cervical cancer cells, intracellular Ce6 uptake of nanophotosensitizers were gradually increased according to the H2O2 concentration, indicating that nanophotosensitizers showed ROS-sensitive delivery of Ce6 against cancer cells.As well as free Ce6, nanophotosensitizers in the absence of light irradiation have low intrinsic cytotoxicity against RAW264.7 cells and HeLa cells. However, nanophotosensitizers induced cell death dose-dependently under light irradiation. Especially, nanophotosensitizers showed significantly higher ROS generation and phototoxicity against HeLa cells in vitro. When nanophotosensitizers were intravenously administered to animal tumor xenograft model of HeLa cells, tumor tissues revealed stronger fluorescence intensity than other tissues by light irradiation while absence of light irradiation induced relatively lower fluorescence intensity in tumor tissues, indicating that nanophotosensitizers have sensitivity against oxidative stress in tumor tissues. We suggest that nanophotosensitizers of mPEGseseCe6PBAP conjugates are promising vehicle for PDT of cervical cancer cells.
Effect of Ultrasonic-Assisted Casting on Hot Deformation Mechanism and Microstructure of 35CrMo Steel Ingot
Authors: Yang Qiumei , Zhou Yajun , Zhang Wei , Zhang Xun , Xu Mengfei
Published: December 2021

Hot compression tests were performed with strain rates (0.01–10 s−1) and temperatures (850–1150 °C). The power law relationship between the critical stress and critical strain and Zener–Hollomon parameters was determined by θ-σ curves. Microstructure was investigated by electron backscattered diffraction. The results showed that the flow behavior and microstructure of 35CrMo steel was affected by ultrasonic-assisted casting. The activation energy of non-ultrasonic and ultrasonic-assisted 35CrMo steel were 410 ± 9.9 and 386 ± 9.4 kJ/mol, respectively, and the activation energy of ultrasonic-assisted specimens was reduced by 6%. In addition, the ultrasonic-assisted treatment refines the grains to some extent and makes the softening process of ultrasonic-assisted samples progress faster, which promoted the development of dynamic recrystallization and the production of Σ3 boundaries. The discontinuous dynamic recrystallization was the main DRX nucleation mechanism of the 35CrMo steel.
Preparation and Characterization of Thermoelectric PEDOT/Te Nanorod Array Composite Films
Authors: Ahn Hong-Ju , Kim Seil , Kim Kwang Ho , Lee Joo-Yul
Published: December 2021

In this study, we prepared Te nanorod arrays via a galvanic displacement reaction (GDR) on a Si wafer, and their composite with poly(3,4-ethylenedioxythiophene) (PEDOT) were successfully synthesized by electrochemical polymerization with lithium perchlorate (LiClO4) as a counter ion. The thermoelectric performance of the composite film was optimized by adjusting the polymerization time. As a result, a maximum power factor (PF) of 235 µW/mK2 was obtained from a PEDOT/Te composite film electrochemically polymerized for 15 s at room temperature, which was 11.7 times higher than that of the PEDOT film, corresponding to a Seebeck coefficient (S) of 290 µV/K and electrical conductivity (σ) of 28 S/cm. This outstanding PF was due to the enhanced interface interaction and carrier energy filtering effect at the interfacial potential barrier between the PEDOT and Te nanorods. This study demonstrates that the combination of an inorganic Te nanorod array with electrodeposited PEDOT is a promising strategy for developing high-performance thermoelectric materials.
A Comparative Analysis of Selected Methods for Determining Young’s Modulus in Polylactic Acid Samples Manufactured with the FDM Method
Authors: Pszczółkowski Bartosz , Nowak Konrad W. , Rejmer Wojciech , Bramowicz Mirosław , Dzadz Łukasz , Gałęcki Remigiusz
Published: December 2021

The objective of this study was to compare three methods for determining the Young’s modulus of polylactic acid (PLA) and acrylonitrile-butadiene-styrene (ABS) samples. The samples were manufactured viathe fused filament fabrication/fused deposition modeling (FFF/FDM) 3D printing technique. Samples for analysis were obtained at processing temperatures of 180 °C to 230 °C. Measurements were performed with the use of two nondestructive techniques: the impulse excitation technique (IET) and the ultrasonic (US) method. The results were compared with values obtained in static tensile tests (STT), which ranged from 2.06 ± 0.03 to 2.15 ± 0.05 GPa. Similar changes in Young’s modulus were observed in response to the processing temperatures of the compared methods. The values generated by the US method were closer to the results of the STT, but still diverged considerably, and the error exceeded 10% in all cases. Based on the present findings, it might be concluded that the results of destructive and nondestructive tests differ by approximately 1 GPa.
Survey of Grain Boundary Energies in Tungsten and Beta-Titanium at High Temperature
Authors: He Hong , Ma Shangyi , Wang Shaoqing
Published: December 2021

Heat treatment is a necessary means to obtain desired properties for most of the materials. Thus, the grain boundary (GB) phenomena observed in experiments actually reflect the GB behaviors at relatively high temperature to some extent. In this work, 405 different GBs were systematically constructed for body-centered cubic (BCC) metals and the grain boundary energies (GBEs) of these GBs were calculated with molecular dynamics for W at 2400 K and β-Ti at 1300 K and by means of molecular statics for Mo and W at 0 K. It was found that high temperature may result in the GB complexion transitions for some GBs, such as the Σ11{332}{332} of W. Moreover, the relationships between GBEs and sin(θ) can be described by the functions of the same type for different GB sets having the same misorientation axis, where θ is the angle between the misorientation axis and the GB plane. Generally, the GBs tend to have lower GBE when sin(θ) is equal to 0. However, the GB sets with the <110> misorientation axis have the lowest GBE when sin(θ) is close to 1. Another discovery is that the local hexagonal-close packed α phase is more likely to form at the GBs with the lattice misorientations of 38.9°/<110>, 50.5°/<110>, 59.0°/<110> and 60.0°/<111> for β-Ti at 1300 K.
On the Miscibility of Nematic Liquid Crystals with Ionic Liquids and Joint Reaction for High Helical Twisting Power Product(s)
Authors: Czajkowski Maciej , Feder-Kubis Joanna , Potaniec Bartłomiej , Duda Łukasz , Cybińska Joanna
Published: December 2021

Mixtures of nematic liquid crystals (LCs) with chiral ionic liquids (CILs) may find application as active materials for electrically driven broadband mirrors. Five nematic liquid crystal hosts were mixed with twenty three ionic liquids, including chiral ones, and studied in terms of their miscibility within the nematic phase. Phase diagrams of the mixtures with CILs which exhibited twisted nematic phase were determined. Miscibility, at levels between 2 and 5 wt%, was found in six mixtures with cyanobiphenyl-based liquid crystal host—E7. On the other hand, the highest changes in the isotropization temperature was found in the mixtures with isothiocyanate-based liquid crystal host—1825. Occurrence of chemical reactions was found. A novel chiral binaphtyl-based organic salt [N11116][BNDP] was synthesized and, in reaction to the 1825 host, resulted in high helical twisting power product(s). Selectivity of the reaction with the isothiocyanate-based liquid crystal was found.
Microanalysis of Worn Surfaces of Selected Rotating Parts of an Internal Combustion Engine
Authors: Bernát Rastislav , Žarnovský Jozef , Kováč Ivan , Mikuš Rastislav , Fries Jiří , Csintalan Radoslav
Published: December 2021

The present paper analyzes the damage of surfaces at spots of frictional contact, namely, the friction nodes on a camshaft and the connecting rod pins of a crankshaft. The resulting wear of the monitored friction nodes reduces the technical life of the machines, which can lead to the decommissioning of the machine. Wear was assessed by measuring roughness and microhardness and by observing the microstructures of the materials. The results of the experiments show that the rotating parts displayed visible wear on the cams, as well as on the connecting rod pins. The experiments revealed that wear was caused by the heating of the material to a high temperature during the operation of the machine and that there was a gradual cooling and tempering of the material, which led to a reduction in the microhardness of the monitored object. Lower microhardness values can be a cause of greater wear of the monitored objects. When comparing the microhardness of the used and the new camshaft, the hardened layer of the new camshaft from secondary production has a significantly smaller thickness compared to worn cams, which leads to the finding of a different material quality compared to the original parts from primary production. This fact indicates that the wear of a new camshaft as a spare part can contribute to the shortening of the technical life of friction nodes.
A Review of Thermal Monitoring Techniques for Radial Permanent Magnet Machines
Authors: Meng Tianze , Zhang Pinjia
Published: December 2021

Permanent magnet machines are widely applied in motor drive systems. Therefore, condition monitoring of permanent magnet machines has great significance to assist maintenance. High temperatures are accountable for lots of typical malfunctions and faults, such as demagnetization of the permanent magnet (PM) and inter-turn short circuit of stator windings. Therefore, temperature monitoring of the PM and stator windings is essential for reliable operation. In this paper, an overview introducing and evaluating existing thermal monitoring methods is presented. First, the mechanism of thermal-caused failures for the PM and stator windings is introduced. Then, the design procedure and principles of existing temperature monitoring methods are introduced and summarized. Next, the evaluations and recommendations of application feasibility are demonstrated. Finally, the potential future challenges and opportunities for temperature monitoring of the PM and stator windings are discussed.
The Advantages of EPR Spectroscopy in Exploring Diamagnetic Metal Ion Binding and Transfer Mechanisms in Biological Systems
Authors: Meron Shelly , Shenberger Yulia , Ruthstein Sharon
Published: December 2021

Electron paramagnetic resonance (EPR) spectroscopy has emerged as an ideal biophysical tool to study complex biological processes. EPR spectroscopy can follow minor conformational changes in various proteins as a function of ligand or protein binding or interactions with high resolution and sensitivity. Resolving cellular mechanisms, involving small ligand binding or metal ion transfer, is not trivial and cannot be studied using conventional biophysical tools. In recent years, our group has been using EPR spectroscopy to study the mechanism underlying copper ion transfer in eukaryotic and prokaryotic systems. This mini-review focuses on our achievements following copper metal coordination in the diamagnetic oxidation state, Cu(I), between biomolecules. We discuss the conformational changes induced in proteins upon Cu(I) binding, as well as the conformational changes induced in two proteins involved in Cu(I) transfer. We also consider how EPR spectroscopy, together with other biophysical and computational tools, can identify the Cu(I)-binding sites. This work describes the advantages of EPR spectroscopy for studying biological processes that involve small ligand binding and transfer between intracellular proteins.
Finite-Time Boundedness of Linear Uncertain Switched Positive Time-Varying Delay Systems with Finite-Time Unbounded Subsystems and Exogenous Disturbance
Authors: Mouktonglang Thanasak , Yimnet Suriyon
Published: December 2021

The problem of finite-time boundedness for a class of linear switched positive time-varying delay systems with interval uncertainties and exogenous disturbance is addressed. This characteristic research is that the studied systems include the finite-time bounded subsystems and finite-time unbounded subsystems. Both a slow mode-dependent average dwell time and a fast mode-dependent average dwell time switching techniques are utilized reasonably. And by applying a copositive Lyapunov-Krasovskii functional, novel delay-dependent sufficient criteria are derived to guarantee such systems to be finite-time bounded concerning the given parameters and designed switching signal. Furthermore, new finite-time boundedness criteria of the systems without interval uncertainties are also obtained. Finally, the efficiency of the theoretical results is presented in two illustrative examples.
Sargachromenol Isolated from Sargassum horneri Inhibits Particulate Matter-Induced Inflammation in Macrophages through Toll-like Receptor-Mediated Cell Signaling Pathways
Authors: Nagahawatta D. P. , Kim Hyun-Soo , Jee Young-Heun , Jayawardena Thilina U. , Ahn Ginnae , Namgung Jin , Yeo In-Kyu , Sanjeewa K. K. Asanka , Jeon You-Jin
Published: December 2021

Sargassum horneri is an invasive brown seaweed that grows along the shallow coastal areas of the Korean peninsula, which are potentially harmful to fisheries and natural habitats in the areas where it is accumulated. Therefore, the author attempted to evaluate the anti-inflammatory mechanism of Sargachromenol isolated from S. horneri against particulate matter (PM)-stimulated RAW 264.7 macrophages. PM is a potent inducer of respiratory diseases such as lung dysfunctions and cancers. In the present study, the anti-inflammatory properties of Sargachromenol were validated using enzyme-linked immunosorbent assay (ELISA), Western blots, and RT-qPCR experiments. According to the results, Sargachromenol significantly downregulated the PM-induced proinflammatory cytokines, Prostaglandin E2 (PGE2), and Nitric Oxide (NO) secretion via blocking downstream activation of Toll-like receptor (TLR)-mediated nuclear factor kappa B (NF-κB) and MAPKs phosphorylation. Thus, Sargachromenol is a potential candidate for innovation in various fields including pharmaceuticals, cosmeceuticals, and functional food.
Structure-Based Pharmacophore Modeling, Virtual Screening, Molecular Docking, ADMET, and Molecular Dynamics (MD) Simulation of Potential Inhibitors of PD-L1 from the Library of Marine Natural Products
Authors: Luo Lianxiang , Zhong Ai , Wang Qu , Zheng Tongyu
Published: December 2021

Background: In the past decade, several antibodies directed against the PD-1/PD-L1 interaction have been approved. However, therapeutic antibodies also exhibit some shortcomings. Using small molecules to regulate the PD-1/PD-L1 pathway may be another way to mobilize the immune system to fight cancer. Method: 52,765 marine natural products were screened against PD-L1(PDBID: 6R3K). To identify natural compounds, a structure-based pharmacophore model was generated, following by virtual screening and molecular docking. Then, the absorption, distribution, metabolism, and excretion (ADME) test was carried out to select the most suitable compounds. Finally, molecular dynamics simulation was also performed to validate the binding property of the top compound. Results: Initially, 12 small marine molecules were screened based on the pharmacophore model. Then, two compounds were selected for further evaluation based on the molecular docking scores. After ADME and toxicity studies, molecule 51320 was selected for further verification. By molecular dynamics analysis, molecule 51320 maintains a stable conformation with the target protein, so it has the chance to become an inhibitor of PD-L1. Conclusions: Through structure-based pharmacophore modeling, virtual screening, molecular docking, ADMET approaches, and molecular dynamics (MD) simulation, the marine natural compound 51320 can be used as a small molecule inhibitor of PD-L1.
A Rare Case of Epithelioid Trophoblastic Tumor Presenting as Hematoma of a Caesarean Scar in the Lower Uterine Segment
Authors: Aničić Radomir , Rakić Aleksandar , Maglić Rastko , Sretenović Dragutin , Ristić Aleksandar , Đaković Elena , Nejković Lazar
Published: December 2021

Epitheliod trophoblastic tumor (ETT) account for only 1–2% of all the cases of gestational trophoblastic neoplasia (GTN), with a reported mortality rate of 10–24%. ETT is derived from chorionic type intermediate trophoblastic cells, which appears to be the reason for the only slightly elevated βhCG levels in these patients. We present a case of a 42-year-old patient who was admitted to the clinic eight months after Caesarean delivery, for irregular vaginal bleed with normal values of beta-human chorionic gonadotropin (βhCG). A 6 × 5 cm hematoma was evacuated from the isthmic uterine segment during the operation, and the histopathological exam of the tissue surrounding the hematoma revealed ETT. There were no metastatic lesions on the thoracal, abdominal, and pelvic CT. The second ultrasonographic exam revealed tumefaction of 5 cm at the site from the previous surgical procedure. Color Doppler imaging revealed no central nor peripheral blood flow. The patient underwent a total abdominal hysterectomy with bilateral adnexectomy without adjuvant chemotherapy. This appears to be one of the shortest intervals from the anteceded gestational event until the diagnosis of this tumor, along with the absence of the significant ultrasonographic feature of the ETT-peripheral Doppler signal pattern. We underline that, even with normal values of βhCG, irregular vaginal bleeding following the antecedent gestational event should always arouse suspicion of GTN.
Malignant Phyllodes Tumor of the Breast and Pregnancy: A Rare Case Report and Literature Review
Authors: Mustață Laura , Gică Nicolae , Botezatu Radu , Chirculescu Raluca , Gică Corina , Peltecu Gheorghe , Panaitescu Anca Maria
Published: December 2021

Phyllodes Tumor (PT) is a rare fibroepithelial breast tumor that can behave differently depending on its biologic features. Traditionally, PTs are classified by their histologic features into benign, borderline, and malignant. In most cases that were reported, all PTs may recur, but only the borderline and malignant PT can metastasize. PT usually occurs as a breast lump or accidental finding on ultrasound (US) examination. The clinical features include a well-defined breast mass, regular or lobulated. The diagnosis is based on the integration of morphology features, but remains challenging, particularly in the distinction from fibroadenomas. We report a case of a 36-year-old patient who presented for a voluminous breast mass, rapidly growing in the past 3–4 months. At presentation, the patient was 19 weeks pregnant. The breast tumor had the clinical and US aspect of PT. A core needle biopsy was obtained, confirming a benign PT, and local excision was performed with no postoperative complications. The final pathology report showed a borderline PT with close resection margins of 1 mm. Immunohistochemistry (IHC) established the diagnosis of malignant PT with heterologous sarcomatous differentiation. The case was discussed in the multidisciplinary tumor board (MDT) and mastectomy was recommended. The patient fully consented but refused surgery at 25 weeks’ gestation, fearing premature delivery. The right breast was closely monitored by US, and at 9 weeks after the first surgery, signs of local recurrence were detected. At 35 weeks’ gestation, right mastectomy was performed, with no perioperative complications. The pregnancy was closely followed up and no complication were found. The final pathology report describes multiples PT recurrences with heterologous sarcomatous differentiation. The pregnancy outcome was uneventful, and the patient delivered a healthy child vaginally at term with no peripartum complication. Postpartum, a computer tomography (CT) examination of the head, thorax, abdomen and pelvis was performed, with no evidence of metastases. Adjuvant chemotherapy and radiotherapy completed the treatment. The follow-up and CT scan showed no metastases or further recurrence 4 years after diagnosis. In conclusion, diagnosis of PT can be difficult, especially because of the easy confusion with fibroadenoma of the breast. There are rare cases when a pathology exam needs further assessment and IHC is recommended for accurate diagnosis. Although malignant PT is rare and accounts for <1% of all breast cancers, the diagnosis and treatment that are recommended are based on the reported cases. Moreover, when complete surgical excision is achieved, the rates of recurrence and distant metastases are low, and adjuvant therapy might not be necessary.
Modeling Asymmetry of a Current–Voltage Curve of a Novel MF-4SC/PTMSP Bilayer Membrane
Authors: Filippov Anatoly N. , Kononenko Natalia A. , Loza Natalia V. , Petrova Daria A.
Published: December 2021

A novel bilayer cation-exchange membrane—consisting of a thick layer of a pristine perfluorinated membrane MF-4SC (Russian equivalent of Nafion®-117) and a thinner layer (1 μm) of the membrane, on a base of glassy polymer of internal microporosity poly(1-trimethylsilyl-1-propyne) (PTMSP)—was prepared and characterized. Using the physicochemical characteristics of one-layer membranes MF-4SC and PTMSP in 0.05 M HCl and NaCl solutions, the asymmetric current–voltage curves (CVC) of the bilayer composite were described with good accuracy up to the overlimiting regime, based on the “fine-porous membrane” model. The MF-4SC/PTMSP bilayer composite has a significant asymmetry of CVC that is promising for using it in electromembrane devices, such as membrane detectors, sensors, and diodes.
Recent Mitigation Strategies on Membrane Fouling for Oily Wastewater Treatment
Authors: Zulkefli Nur Fatihah , Alias Nur Hashimah , Jamaluddin Nur Shafiqah , Abdullah Norfadhilatuladha , Abdul Manaf Shareena Fairuz , Othman Nur Hidayati , Marpani Fauziah , Mat-Shayuti Muhammad Shafiq , Kusworo Tutuk Djoko
Published: December 2021

The discharge of massive amounts of oily wastewater has become one of the major concerns among the scientific community. Membrane filtration has been one of the most used methods of treating oily wastewater due to its stability, convenience handling, and durability. However, the continuous occurrence of membrane fouling aggravates the membrane’s performance efficiency. Membrane fouling can be defined as the accumulation of various materials in the pores or surface of the membrane that affect the permeate’s quantity and quality. Many aspects of fouling have been reviewed, but recent methods for fouling reduction in oily wastewater have not been explored and discussed sufficiently. This review highlights the mitigation strategies to reduce membrane fouling from oily wastewater. We first review the membrane technology principle for oily wastewater treatment, followed by a discussion on different fouling mechanisms of inorganic fouling, organic fouling, biological fouling, and colloidal fouling for better understanding and prevention of membrane fouling. Recent mitigation strategies to reduce fouling caused by oily wastewater treatment are also discussed.
Crosslinking Multilayer Graphene by Gas Cluster Ion Bombardment
Authors: Almassov Nurlan , Kirkpatrick Sean , Alsar Zhanna , Serik Nurzhan , Spitas Christos , Kostas Konstantinos , Insepov Zinetula
Published: December 2021

In this paper, we demonstrate a new, highly efficient method of crosslinking multilayer graphene, and create nanopores in it by its irradiation with low-energy argon cluster ions. Irradiation was performed by argon cluster ions with an acceleration energy E ≈ 30 keV, and total fluence of argon cluster ions ranging from 1 × 109 to 1 × 1014 ions/cm2. The results of the bombardment were observed by the direct examination of traces of argon-cluster penetration in multilayer graphene, using high-resolution transmission electron microscopy. Further image processing revealed an average pore diameter of approximately 3 nm, with the predominant size corresponding to 2 nm. We anticipate that a controlled cross-linking process in multilayer graphene can be achieved by appropriately varying irradiation energy, dose, and type of clusters. We believe that this method is very promising for modulating the properties of multilayer graphene, and opens new possibilities for creating three-dimensional nanomaterials.
Hydrometallurgical Recycling of Copper Anode Furnace Dust for a Complete Recovery of Metal Values
Authors: Oráč Dušan , Klimko Jakub , Klein Dušan , Pirošková Jana , Liptai Pavol , Vindt Tomáš , Miškufová Andrea
Published: December 2021

Copper anode furnace dust is waste by-product of secondary copper production containing zinc, lead, copper, tin, iron and many other elements. Hydrometallurgical Copper Anode Furnace dust recycling method was studied theoretically by thermodynamic calculations and the proposed method was verified experimentally on a laboratory scale. The optimum condition for leaching of zinc from dust was identified to be an ambient leaching temperature, a liquid/solid ratio of 10 and H2SO4 concentration of 1 mol/L. A maximum of 98.85% of zinc was leached under the optimum experimental conditions. In the leaching step, 99.7% of lead in the form of insoluble PbSO4 was separated from the other leached metals. Solution refining was done by combination of pH adjustment and zinc powder cementation. Tin was precipitated from solution by pH adjustment to 3. Iron was precipitated out of solution after pH adjustment to 4 with efficiency 98.54%. Copper was selectively cemented out of solution (99.96%) by zinc powder. Zinc was precipitated out of solution by addition of Na2CO3 with efficiency of 97.31%. ZnO as final product was obtained by calcination of zinc carbonates.
The Effects of Deteriorated Boundary Conditions on Horizontally Framed Miter Gates
Authors: Riveros Guillermo A. , Acosta Felipe J. , Lozano Christine M. , Glynn Eileen
Published: December 2021

The U.S. navigable infrastructure is a system of waterways dependent upon hydraulic steel structures (HSS) to facilitate the passage of ships and cargo. The system is linear in the sense that if one HSS is impassable, the entire river system is halted at that point. The majority of the HSS in this system were built in the first half of the 20th Century, and over seventy percent of them are past or near past their design life. Miter gates are critical HSS components within the system and many are showing signs of structural distress from continued operation past their design life. Common distress features include shear cracking within the pintle socket, partially missing Quoin blocks, fatigue fracturing, and bolt failure in the pintle region. This article focuses on gaining a fundamental understanding of the consequences of quoin block deterioration on a miter gate. The work was conducted by developing a computational model of a miter gate with different levels of quoin block deterioration. This model was validated using analytical solutions. The deterioration results demonstrated that the miter gate thrust diaphragm and quoin post experienced changes in their limit states due to deterioration. The results also demonstrated that the miter gate could overcome up to 10% of quoin block deterioration.