bluuebunny/crossref_metadata_2025 huggingface dataset:
Soil Enzyme Stoichiometry and Its Influencing Factors in Different Mangrove Communities in Xinying Bay, Hainan
Authors: Liu Chang , Zhong Lishuang , Li Donghai , Zhang Xiang , Yang Xiaobo , Chen Yuepeng , Qi Chunlin
Published: December 2024

To investigate the differences in soil hydrolytic enzyme activity and enzyme stoichiometry among different mangrove communities, as well as the driving factors behind these variations, we will explore their implications for nutrient limitations of soil microbes and the availability of soil nutrients. This study will focus on the Rhizophora stylosa communities (RS), Aegiceras corniculatum communities (AC), and RS-AC mixed communities (MF) within the Hainan Xinying Bay mangrove conservation area, using adjacent bare flats (BF) as a control group. We will analyse soil enzyme activity and physicochemical properties in at soil depths of 0–20 cm and 20–40 cm across different mangrove communities to uncover the synergistic changes between these variables. The results indicate the following. (1) Except for acid phosphatase (ACP), the enzyme activities and their stoichiometric ratios in different mangrove soil communities differ significantly. In the layer of soil 0~20 cm, β-glucosidase (BG) activity is markedly diminished in the MF community relative to the other communities. Conversely, the activities of β-1,4-N-acetylglucosaminidase (NAG) and leucine aminopeptidase (LAP) are the most elevated in MF. In the 20~40 cm soil layer, the activity of cellulase (CBH) was found to be highest in the MF community, while the activities of BG and NAG in MF were significantly lower than those in other communities. Overall, the activity of the five enzymes decreased with increasing soil depth. (2) The ratios of ln (BG + CBH)/ln (NAG + LAP) and ln (BG + CBH)/ln (ACP) in different soil layers indicated that MF was significantly lower than other communities; in the 0~20 cm soil layer, the ln (NAG + LAP)/ln (ACP) ratio was highest in MF and lowest in RS, while no significant differences were observed between BF and AC. In the 20~40 cm soil layer, no significant differences in ln (NAG + LAP)/ln (ACP) ratios were found among the different communities. (3) The vector angles of the soil enzymatic stoichiometry in the three different mangrove communities and light beaches are all less than 45°. (4) The soil enzyme C:N:P ratio, after logarithmic transformation, measures at 1:1.36:1.28, deviating from the global average (1:1:1). This, in combination with the enzymatic stoichiometry, suggests that nitrogen and phosphorus both constrain the soil microorganisms in this study region, with nitrogen constraining them more strongly. Redundancy analysis indicates that the soil TK content is a primary driver regulating soil enzyme activity and its stoichiometric ratios. From the perspective of accelerating nutrient cycling and alleviating nutrient limitation, this study found that increasing exogenous inputs of nitrogen and potassium can alleviate nitrogen and phosphorus limitation in the mangrove ecosystem of Xinying Bay. These findings provide a basic theoretical basis for restoring and maintaining a healthy tropical mangrove ecosystem.
Predicting Forest Evapotranspiration Shifts Under Diverse Climate Change Scenarios by Leveraging the SEBAL Model Across Inner Mongolia
Authors: Ji Penghao , Su Rong , Gao Runhong
Published: December 2024

This study examines climate change impacts on evapotranspiration in Inner Mongolia, analyzing potential (PET) and actual (AET) evapotranspiration shifts across diverse land-use classes using the SEBAL model and SSP2-4.5 and SSP5-8.5 projections (2030–2050) relative to a 1985–2015 baseline. Our findings reveal substantial PET increases across all LULC types, with Non-Vegetated Lands consistently showing the highest absolute PET values across scenarios (931.19 mm under baseline, increasing to 975.65 mm under SSP5-8.5) due to limited vegetation cover and shading effects, while forests, croplands, and savannas exhibit the most pronounced relative increases under SSP5-8.5, driven by heightened atmospheric demand and vegetation-induced transpiration. Monthly analyses show pronounced PET increases, particularly in the warmer months (June–August), with projected SSP5-8.5 PET levels reaching peaks of over 500 mm, indicating significant future water demand. AET increases are largest in densely vegetated classes, such as forests (+242.41 mm for Evergreen Needleleaf Forests under SSP5-8.5), while croplands and grasslands exhibit more moderate gains (+249.59 mm and +167.75 mm, respectively). The widening PET-AET gap highlights a growing vulnerability to moisture deficits, particularly in croplands and grasslands. Forested areas, while resilient, face rising water demands, necessitating conservation measures, whereas croplands and grasslands in low-precipitation areas risk soil moisture deficits and productivity declines due to limited adaptive capacity. Non-Vegetated Lands and built-up areas exhibit minimal AET responses (+16.37 mm for Non-Vegetated Lands under SSP5-8.5), emphasizing their limited water cycling contributions despite high PET. This research enhances the understanding of climate-induced changes in water demands across semi-arid regions, providing critical insights into effective and region-specific water resource management strategies.
Reframing Forest Harvest Scheduling Models for Ecosystem Services Management
Authors: Nobre Silvana Ribeiro , McDill Marc Eric , Rodriguez Luiz Carlos Estraviz , Diaz-Balteiro Luis
Published: December 2024

Linear programming models have been used in forest management planning since the 1960s. These models have been formulated in three basic ways: Models I, II, and III, which are defined by the sequences of management unit states represented by the variables. In Model I, variables represent sequences of states from the beginning of the planning horizon to the end. In Model II, variables represent sequences of states from one intervention to the next. Finally, in Model III, variables represent a single arc in a management unit’s decision tree, i.e., two states. The objectives of this paper are to clarify the definitions of these model variations and evaluate the advantages and disadvantages of each model. This second objective is to test the hypothesis that the relative performance of these models varies with the increasing number of ecosystem services (ES) incorporated into the models. This objective was achieved by formulating a case study problem using each model type. The case study includes three increasingly complex scenarios, each incorporating additional ecosystem services. Results show that despite having more variables and constraints, Model III requires the least time to formulate due to its less dense parameter matrix. Model II has the shortest solution times, followed closely by Model III, while Model I requires the longest times for both formulation and solution. These results are increasingly apparent in more complex scenarios.
The Combined Effects of Salt and Nitrogen Addition on the Chlorophyll Fluorescence, Antioxidant System, and Leaf Stoichiometry of Torreya grandis Sexes
Authors: Liu Yang , Chen Danyang , Zhang Haochen , Jin Songheng
Published: December 2024

Previous studies have shown that there are significant sexual differences in the physiological responses of Torreya grandis to environmental stress. However, little is known about its sex-specific differences in response to salt stress against the background of nitrogen (N) deposition. In this experiment, two-year-old male and female T. grandis seedlings were used as experimental materials and exposed to moderate salt conditions and different N levels to study the effects of nitrogen addition and salt stress on the chlorophyll content, chlorophyll fluorescence parameters, antioxidant system, and leaf stoichiometry of T. grandis seedlings. With the increase in nitrogen content, the contents of proline, malondialdehyde, superoxide anion, and H2O2 in the leaves of T. grandis seedlings under salt conditions gradually increased. The contents of these four metabolites in the leaves of male T. grandis seedlings were almost all higher than those of the female ones. Compared with the control group, the contents of enzymatic and non-enzymatic antioxidants increased under N addition treatments, especially for the low and moderate N addition groups. The results showed that moderate concentrations of N addition can mitigate the damage caused by salt, while high concentrations of nitrogen do not. Under conditions of salt and nitrogen addition, female T. grandis seedlings outperformed male ones, as evidenced by their higher photosynthetic pigment content, enhanced antioxidant enzyme activity, reduced accumulation of intracellular cytotoxic metabolites, and higher carbon and nitrogen content in their leaves compared to those of male seedlings. The findings of this research will contribute to our understanding and offer a theoretical foundation for the cultivation of T. grandis seedlings in environments with nitrogen deposition and salinization.
Thermoelectric Generator-Powered Long-Range Wireless Real-Time Steam Leak Detection in Steam Traps
Authors: Aragonés Raúl , Oliver Joan , Ferrer Carles
Published: December 2024

This paper introduces a novel thermoelectric generator (TEG)-powered Industrial Internet of Things (IIoT) device that addresses key limitations in the detection of steam leaks in industrial steam pipelines, particularly in steam traps. Existing solutions often rely on battery-powered or wired sensors, which are limited by high maintenance costs, short lifespans, or significant infrastructure investments. The proposed device operates without batteries, using waste heat to provide continuous power, and leverages LoRaWAN for long-range wireless communication, minimizing reliance on costly internal infrastructure. Additionally, the device integrates temperature differential (ΔT) and ultrasonic sensors with edge computing capabilities to enhance real-time leak detection and reduce dependency on cloud computing. By enabling precise, low-maintenance monitoring of steam systems in energy-intensive industries (e.g., petrochemical, pharmaceutical), this technology can significantly reduce energy losses, operational costs, and greenhouse gas emissions. Initial testing demonstrates the device’s ability to detect leaks accurately under varying industrial conditions, offering a robust, scalable solution for Industry 4.0 applications.
Bioactive Peptides and Evaluation of Cardiac Cytoprotective Effects of Red Millet Yellow Wine as Functional Food
Authors: He Zehui , Zhou Yu , Li Shuang , Li Wen , Zhang Yingxin , Guo Cancan , Guo Zexin , Wei Bo , Bi Yuefeng
Published: December 2024

Red millet yellow wine, a functional beverage fermented from grain, has physiological functions including relieving cardiovascular diseases. However, the active components and mechanism of red millet yellow wine largely remain to be elucidated. In this study, bioactive peptides in red millet yellow wine and the cardiac cytoprotective effects were first investigated. A single-factor test and response surface method were used to optimize the solvent precipitation method to purify bioactive peptides. The final peptide content was up to 72.23%. Analysis of liquid chromatography–tandem mass spectrometry indicated a high antioxidative potential of the identified peptides. Multiple activity assays in vitro revealed that red millet yellow wine (1 mg/mL), particularly peptides (0.1 mg/mL), could protect H9c2 cells from H2O2-induced oxidative damage, thereby improving cell viability. At the mechanistic level, the antioxidant effect of bioactive peptides was achieved through strengthening antioxidative stress capacity and attributed to the activation of the Sirt1/Nrf2 pathway, indicating that peptides may be the main active components responsible for the cardiac cytoprotective effects of red millet yellow wine. These results are expected to provide a reference for further exploration of the health benefits of red millet yellow wine.
Multiplex Detection of SNPs for Genetic Monitoring in Laboratory Mice by Luminex xTAG Assay
Authors: Zhou Jiaqi , Wei Jie , Wang Hong , Li Huan , Zhao Lan , Fu Rui , Yue Bingfei
Published: December 2024

Background: The genetic quality of laboratory mice may have a direct impact on the results of research. Therefore, it is essential to improve genetic monitoring methods to guarantee research quality. However, few current methods boast high efficiency, high throughput, low cost, and general applicability at the same time. Methods: First, we got 34 SNP loci from previous studies for inbred strains and screened out 15 loci with good polymorphism for outbred groups from these 34 loci. Then, by using the Luminex xTAG assay, we tested inbred strains and outbred groups. Results: We tested commonly used inbred strains and five DNA samples from the International Council for Laboratory Animal Science, obtaining correct genotyping results. Additionally, some loci were potentially confirmed to be useful for distinguishing C57BL/6 and BALB/c mouse substrains. Furthermore, we tested three outbred groups and analyzed the genetic structure, and we compared the results of the SNP markers by xTAG assay to the STR markers by PCR, the trends of the three groups are the same. Conclusions: In our studies, the panels could meet the requirements for method promotion and provide a good choice for the genetic monitoring of inbred and outbred mice.
Side- and Disease-Dependent Changes in Human Aortic Valve Cell Population and Transcriptomic Heterogeneity Determined by Single-Cell RNA Sequencing
Authors: Villa-Roel Nicolas , Park Christian , Andueza Aitor , Baek Kyung In , Su Ally , Blaser Mark C. , Leshnower Bradley G. , Yoganathan Ajit , Aikawa Elena , Jo Hanjoong
Published: December 2024

Background: Calcific aortic valve disease (CAVD) is a highly prevalent disease, especially in the elderly population, but there are no effective drug therapies other than aortic valve repair or replacement. CAVD develops preferentially on the fibrosa side, while the ventricularis side remains relatively spared through unknown mechanisms. We hypothesized that the fibrosa is prone to the disease due to side-dependent differences in transcriptomic patterns and cell phenotypes. Methods: To test this hypothesis, we performed single-cell RNA sequencing using a new method to collect endothelial-enriched samples independently from the fibrosa and ventricularis sides of freshly obtained human aortic valve leaflets from five donors, ranging from non-diseased to fibrocalcific stages. Results: From the 82,356 aortic valve cells analyzed, we found 27 cell clusters, including seven valvular endothelial cell (VEC), nine valvular interstitial cell (VIC), and seven immune, three transitional, and one stromal cell population. We identified several side-dependent VEC subtypes with unique gene expression patterns. Homeostatic VIC clusters were abundant in non-diseased tissues, while VICs enriched with fibrocalcific genes and pathways were more prevalent in diseased leaflets. Furthermore, homeostatic macrophage (MΦ) clusters decreased while inflammatory MΦ and T-cell clusters increased with disease progression. A foamy MΦ cluster was increased in the fibrosa of mildly diseased tissues. Some side-dependent VEC clusters represented non-diseased, protective phenotypes, while others were CAVD-associated and were characterized by genes enriched in pathways of inflammation, endothelial–mesenchymal transition, apoptosis, proliferation, and fibrosis. Interestingly, we found several activator protein-1 (AP-1)-related transcription factors (FOSB, FOS, JUN, JUNB) and EGR1 to be upregulated in the fibrosa and diseased aortic valve leaflets. Conclusions: Our results showed that VECs are highly heterogeneous in a side- and CAVD-dependent manner. Unique VEC clusters and their differentially regulated genes and pathways found in the fibrosa of diseased tissues may represent novel pathogenic mechanisms and potential therapeutic targets.
The Impact of Bevacizumab and miR200c on EMT and EGFR-TKI Resistance in EGFR-Mutant Lung Cancer Organoids
Authors: Kobayashi Nobuaki , Katakura Seigo , Fukuda Nobuhiko , Somekawa Kohei , Kaneko Ayami , Kaneko Takeshi
Published: December 2024

Objectives: This research aims to investigate the mechanisms of resistance to epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKIs) in non-small-cell lung cancer (NSCLC), particularly focusing on the role of the epithelial–mesenchymal transition (EMT) within the tumor microenvironment (TME). Materials and Methods: We employed an in vitro three-dimensional organoid model that mirrors the physiology of human lung cancer. These organoids consist of lung cancer cells harboring specific EGFR mutations, human mesenchymal stem cells, and human umbilical vein endothelial cells. We analyzed EMT and drug resistance markers, and evaluated the effects of the anti-angiogenic agent Bevacizumab and micro-RNA miR200c. Results: The study identified a significant link between EMT and EGFR-TKI resistance. Notable findings included a decrease in E-cadherin and an increase in Zinc Finger E-Box Binding Homeobox 1 (ZEB1), both of which influenced EMT and resistance to treatment. Bevacizumab showed promise in improving drug resistance and mitigating EMT, suggesting an involvement of the Vascular Endothelial Growth Factor (VEGF) cascade. Transfection with miR200c was associated with improved EMT and drug resistance, further highlighting the role of EMT in TKI resistance. Conclusions: Our research provides significant insights into the EMT-driven EGFR-TKI resistance in NSCLC and offers potential strategies to overcome resistance, including the use of Bevacizumab and miR200c. However, due to the limitations in organoid models in replicating precise human cancer TME and the potential influence of specific EGFR mutations, further in vivo studies and clinical trials are necessary for validation.
An Assessment of Multipollutant Exposures Using Silicone Wristbands Among Bangladeshi Youth
Authors: Quaid Margaret , Haque Syed Emdadul , Islam Tariqul , Shahriar Mohammad Hasan , Sarwar Golam , Ahmed Alauddin , O’Connell Steven , Jasmine Farzana , Kibriya Muhammad G. , Ahsan Habibul , Argos Maria
Published: December 2024

Residents of Bangladesh are exposed to numerous chemicals due to local industries, including dyeing mills, cotton mills, and the use of biomass in daily cooking. It is, therefore, important to characterize the exposome and work to identify risk factors of exposure. We used silicone wristband passive samplers to evaluate exposure to volatile and semi-volatile organic compounds in a sample of 40 children in the Araihazar upazila of Bangladesh. We used stepwise linear regression models to determine which demographic, exposure, diet, and socioeconomic factors best predict exposure to single chemicals and classes of chemicals. Male sex at birth was associated with a decrease in the number of chemicals detected above their median concentration (β = −2.42; 95%CI: −5.24, 0.399), as was ownership of a flush toilet (β = −3.26; 95%CI: −6.61, 0.097). Increased body mass index (β = 1.81; 95%CI: 0.587, 3.03), father’s smoking (β = 2.74; 95%CI: −0.0113, 5.49), and father’s employment in the garment industry (β = 3.14; 95%CI: 0.209, 6.07) were each associated with an increase in the average number of chemicals detected above their median concentration. The observed results motivate future evaluation with health outcomes of these exposures.
Advances in Molecular Research of Tropical Fruit
Authors: Qin Yonghua
Published: December 2024

Fruit trees, similar to other edible plants, hold immense commercial value within the agricultural sector [...]
Emerging Signatures of Hematological Malignancies from Gene Expression and Transcription Factor-Gene Regulations
Authors: Dall’Olio Daniele , Magnani Federico , Casadei Francesco , Matteuzzi Tommaso , Curti Nico , Merlotti Alessandra , Simonetti Giorgia , Della Porta Matteo Giovanni , Remondini Daniel , Tarozzi Martina , Castellani Gastone
Published: December 2024

Hematological malignancies are a diverse group of cancers developing in the peripheral blood, the bone marrow or the lymphatic system. Due to their heterogeneity, the identification of novel and advanced molecular signatures is essential for enhancing their characterization and facilitate its translation to new pharmaceutical solutions and eventually to clinical applications. In this study, we collected publicly available microarray data for more than five thousand subjects, across thirteen hematological malignancies. Using PANDA to estimate gene regulatory networks (GRNs), we performed hierarchical clustering and network analysis to explore transcription factor (TF) interactions and their implications on biological pathways. Our findings reveal distinct clustering patterns among leukemias and lymphomas, with notable differences in gene and TF expression profiles. Gene Set Enrichment Analysis (GSEA) identified 57 significantly enriched KEGG pathways, highlighting both common and unique biological processes across HMs. We also identified potential drug targets within these pathways, emphasizing the role of TFs such as CEBPB and NFE2L1 in disease pathophysiology. Our comprehensive analysis enhances the understanding of the molecular landscape of HMs and suggests new avenues for targeted therapeutic strategies. These findings also motivate the adoption of regulatory networks, combined with modern biotechnological possibilities, for insightful pan-cancer exploratory studies.
Functional Involvement of Signal Transducers and Activators of Transcription in the Pathogenesis of Influenza A Virus
Authors: Liu Shasha , Qiu Feng , Gu Rongrong , Xu Erying
Published: December 2024

Signal transducers and activators of transcription (STATs) function both as signal transducers and transcription regulators. STAT proteins are involved in the signaling pathways of cytokines and growth factors; thus, they participate in various life activities and play especially critical roles in antiviral immunity. Convincing evidence suggests that STATs can establish innate immune status through multiple mechanisms, efficiently eliminating pathogens. STAT1 and STAT2 can activate the antiviral status by regulating the interferon (IFN) signal. In turn, suppressor of cytokine signaling-1 (SOCS1) and SOCS3 can modulate the activation of STATs and suppress the excessive antiviral immune response. STAT3 not only regulates the IFN signal, but also transduces Interleukin-6 (IL-6) to stimulate the host antiviral response. The function of STAT4 and STAT5 is related to CD4+ T helper (Th) cells, and the specific mechanism of STAT5 remains to be studied. STAT6 mainly exerts antiviral effects by mediating IL-4 and IL-13 signaling. Here, we reviewed the recent findings regarding the critical roles of STATs in the interactions between the host and viral infection, especially influenza A virus (IAV) infection. We also discuss the molecular mechanisms underlying their functions in antiviral responses.
SNPs in GPCR Genes and Impaired Osteogenic Potency in Osteoporotic Patient Lines-Based Study
Authors: Sopova Julia , Krasnova Olga , Vasilieva Giomar , Zhuk Anna , Lesnyak Olga , Karelkin Vitaliy , Neganova Irina
Published: December 2024

G-protein-coupled receptors (GPCRs) have emerged as critical regulators of bone development and remodeling. In this study, we aimed to identify specific GPCR mutations in osteoporotic patients via next-generation sequencing (NGS). We performed NGS sequencing of six genomic DNA samples taken from osteoporotic patients and two genomic DNA samples from healthy donors. Next, we searched for single-nucleotide polymorphisms (SNPs) in GPCR genes that are associated with osteoporosis. For three osteoporotic patients and one healthy donor, bone biopsies were used to generate patient-specific mesenchymal stem cell (MSC) lines, and their ability to undergo osteodifferentiation was analyzed. We found that MSCs derived from osteoporotic patients have a different response to osteoinductive factors and impaired osteogenic differentiation using qPCR and histochemical staining assays. The NGS analysis revealed specific combinations of SNPs in GPCR genes in these patients, where SNPs in ADRB2 (rs1042713), GIPR (rs1800437), CNR2 (rs2501431, rs3003336), and WLS (rs3762371) were associated with impaired osteogenic differentiation capacity. By integrating NGS data with functional assessments of patient-specific cell lines, we linked GPCR mutations to impaired bone formation, providing a foundation for developing personalized therapeutic strategies. SNP analysis is recognized as a proactive approach to osteoporosis management, enabling earlier interventions and targeted preventive measures for individuals at risk. Furthermore, SNP analysis contributes to the development of robust, holistic risk prediction models that enhance the accuracy of risk assessments across the population. This integration of genetic data into public health strategies facilitates healthcare initiatives. This approach could guide treatment decisions tailored to the patient’s genetic profile and provide a foundation for developing personalized therapeutic strategies.
Current Insights into the Roles of LncRNAs and CircRNAs in Pulpitis: A Narrative Review
Authors: Fuchen-Ramos Dulce Martha , Leija-Montoya Ana Gabriela , González-Ramírez Javier , Isiordia-Espinoza Mario , García-Arévalo Fernando , Pitones-Rubio Viviana , Olvera-Sandoval Carlos , Mateos-Corral Isis , Serafín-Higuera Nicolás
Published: December 2024

Pulpitis, an inflammation of the dental pulp, is generated by bacterial invasion through different ways as caries. In the establishment and development of this disease, different biological processes are involved. Long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) are transcripts with regulatory capacity participating in different biological functions and have been implicated in different diseases. The aim of this narrative review is to critically analyze available evidence on the biological role of lncRNAs and circRNAs in pulpitis and discuss possible new research prospects. LncRNAs and circRNAs involved in pulpitis were explored, addressing their expression, molecular mechanisms, targets and biological effects studied in animal and in vitro models, as well as in studies in human patients. LncRNAs and circRNAs are emerging as key regulators of diverse biological functions in pulpitis including apoptosis, proliferation, differentiation, oxidative stress, autophagy, ferroptosis, inflammation and immune response. The molecular mechanisms performed by these non-coding RNAs (ncRNAs) involved interactions with miRNAs and the formation of regulatory networks in the context of pulpitis. Further studies more deeply analyzing the participation of lncRNAs and circRNAs in pulpitis will reveal the potential applications of these ncRNAs as biomarkers or their use in therapeutic strategies in pulp inflammation.
Characterization of Dendritic Cells and Myeloid-Derived Suppressor Cells Expressing Major Histocompatibility Complex Class II in Secondary Lymphoid Organs in Systemic Lupus Erythematosus-Prone Mice
Authors: Uribe Felipe R. , González-Martínez Fabián , Echeverría-Araya Sebastián A. , Sepúlveda-Pontigo Alison , Chávez-Villacreses Karissa , Díaz-Bozo Andrés , Méndez-Pérez Isabel , González Valentina P. I. , Bohmwald Karen , Kalergis Alexis M. , Soto Jorge A.
Published: December 2024

Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by self-antibody production and widespread inflammation affecting various body tissues. This disease is driven by the breakdown of immune tolerance, which promotes the activation of autoreactive B and T cells. A key feature of SLE is dysregulation in antigen presentation, where antigen-presenting cells (APCs) play a central role in perpetuating immune responses. Dendritic cells (DCs) are highly specialized for antigen presentation among APCs. At the same time, myeloid-derived suppressor cells (MDSCs) can also express MHC-II molecules, although their role in SLE is less understood. Utilizing the SLE model, MRL/MpJ-Faslpr/J, we determined the presence of different phenotypes of DCs and MDSCs expressing MHC-II in secondary lymphoid organs, along with the gene expression of ICOSL, CD80 and CD86 in the spleen. Our study determined that the most abundant population of APCs in secondary lymphoid organs corresponds to cDC CD103−CD11b+ MHC-II+ throughout SLE development. Additionally, ICOSL expression increased over time, becoming more preponderant in week 16 in the SLE model, which could indicate that it is a crucial pathway for the development and progression of the pathology. In week 16, we observed a positive correlation between M-MDSC MHC-II and IFN-γ-producing CD4+ T cells.
The Effect of Intravascular Laser Irradiation of Blood on Serum Biomarkers and Clinical Outcome in Knee Osteoarthritis Patients: A Double-Blind Randomized Control Trial
Authors: Su Yu-Chi , Shen Yu-Ping , Chang Chih-Ya , Pan Ke-Ting , Huang Shih-Ming , Chen Liang-Cheng
Published: December 2024

Knee osteoarthritis (OA) is a prevalent degenerative joint disease globally, causing pain, stiffness, and disability. Intravascular laser irradiation of blood (ILIB) has been used for chronic pain and musculoskeletal disease. However, evidence on the clinical benefits and serum biomarkers post-ILIB therapy in knee OA is insufficient. We designed a double-blind randomized controlled trial to evaluate the clinical and biological outcomes of ILIB therapy for knee OA. Seventeen patients with knee OA were randomly assigned to the ILIB and control groups. The outcomes included the Western Ontario and McMaster Universities Osteoarthritis (WOMAC) Scale, visual analog scale, and biomarker analysis of interleukin (IL)-6, IL-13, IL-1β, epidermal growth factor, macrophage inflammatory protein-1β, and eotaxin. The measurements were performed at baseline and three days, one month, and three months post-intervention. The ILIB group showed a significant improvement in the WOMAC-pain score at one month of follow-up than the control group. IL-1β levels reduced significantly on day three, one month, and three months, and IL-13 levels reduced on day three and three months during follow-up in the ILIB group. ILIB therapy reduced knee OA pain for one month and significantly reduced serum IL-1β and IL-13 levels, suggesting potential for pain management.
Tumorigenesis Caused by Aberrant Expression of GANP, a Central Component in the Mammalian TREX-2 Complex—Lessons from Transcription-Coupled DNA Damages
Authors: Rezano Andri , Gondo Naomi , Sakai Yasuhiro , Nakamura Yuko , Phimsen Suchada , Tani Tokio , Ito Akihiko , Okada Seiji , Kuwahara Kazuhiko
Published: December 2024

DNA is frequently damaged by genotoxic stresses such as ionizing radiation, reactive oxygen species, and nitrogen species. DNA damage is a key contributor to cancer initiation and progression, and thus the precise and timely repair of these harmful lesions is required. Recent studies revealed transcription as a source of genome instability, and transcription-coupled DNA damage has been a focus in cancer research. Impaired mRNA export is closely related to DNA damage through R-loop formation. The molecular machineries of transcription-coupled DNA damage have been extensively analyzed in Saccharomyces cerevisiae. However, the molecular basis of these phenomena in higher eukaryotes remains elusive. In this review, we focus on the relationship between deregulated mRNA export through the transcription-export-2 (TREX-2) complex and cancer development. Particularly, the expression of germinal center-associated nuclear protein (GANP), a molecular scaffold in the TREX-2 complex, is highly associated with tumorigenesis in mice and humans. Although the deregulated expression of other components in the TREX-2 complex might affect cancer development, we have directly demonstrated the significance of GANP in tumorigenesis using genetically modified mice. Additionally, we describe recent evidence for medical applications demonstrating that the downregulation of the other components may be a good candidate for a chemotherapeutic target in terms of reducing the side effects.
Revolutionizing Neuroimmunology: Unraveling Immune Dynamics and Therapeutic Innovations in CNS Disorders
Authors: Toader Corneliu , Tataru Calin Petru , Munteanu Octavian , Covache-Busuioc Razvan-Adrian , Serban Matei , Ciurea Alexandru Vlad , Enyedi Mihaly
Published: December 2024

Neuroimmunology is reshaping the understanding of the central nervous system (CNS), revealing it as an active immune organ rather than an isolated structure. This review delves into the unprecedented discoveries transforming the field, including the emerging roles of microglia, astrocytes, and the blood–brain barrier (BBB) in orchestrating neuroimmune dynamics. Highlighting their dual roles in both repair and disease progression, we uncover how these elements contribute to the intricate pathophysiology of neurodegenerative diseases, cerebrovascular conditions, and CNS tumors. Novel insights into microglial priming, astrocytic cytokine networks, and meningeal lymphatics challenge the conventional paradigms of immune privilege, offering fresh perspectives on disease mechanisms. This work introduces groundbreaking therapeutic innovations, from precision immunotherapies to the controlled modulation of the BBB using nanotechnology and focused ultrasound. Moreover, we explore the fusion of immune modulation with neuromodulatory technologies, underscoring new frontiers for personalized medicine in previously intractable diseases. By synthesizing these advancements, we propose a transformative framework that integrates cutting-edge research with clinical translation, charting a bold path toward redefining CNS disease management in the era of precision neuroimmunology.
The Potential Regulatory Role of Ferroptosis in Orthodontically Induced Inflammatory Root Resorption
Authors: Wang Leilei , Wang Chuan , He Hong
Published: December 2024

People, in increasing numbers, are seeking orthodontic treatment to correct malocclusion, while some of them are suffering from orthodontically induced inflammatory root resorption (OIIRR). Recent evidence suggests that the immune-inflammatory response occurring during bone remodeling may be responsible for OIIRR. Ferroptosis, a new type of programmed cell death (PCD), has been found to have a close interrelation with inflammation during disease progression. While ferroptosis has been extensively studied in bone-related diseases, its role in OIIRR is poorly understood. Considering that the tooth root shares a lot of similar characteristics with bone, it is reasonable to hypothesize that ferroptosis contributes to the development of OIIRR. Nevertheless, direct evidence supporting this theory is currently lacking. In this review, we introduced ferroptosis and elucidated the mechanisms underlying orthodontic tooth movement (OTM) and OIIRR, with a special focus on the pivotal role inflammation plays in these processes. Additionally, we covered recent research exploring the connections between inflammation and ferroptosis. Lastly, we emphasized the important regulatory function of ferroptosis in bone homeostasis. Further investigations are required to clarify the modulation mechanisms of ferroptosis in OIIRR and to develop novel and potential therapeutic strategies for the management of OIIRR.
Genetic Diversity and Fingerprinting of 231 Mango Germplasm Using Genome SSR Markers
Authors: Yan Jinyuan , Zheng Bin , Wang Songbiao , Xu Wentian , Qian Minjie , Ma Xiaowei , Wu Hongxia
Published: December 2024

Mango (Mangifera indica L.) (2n = 40) is an important perennial fruit tree in tropical and subtropical regions. The lack of information on genetic diversity at the molecular level hinders efforts in mango genetic improvement and molecular marker-assisted breeding. In this study, a genome-wide screening was conducted to develop simple sequence repeat (SSR) markers using the Alphonso reference genome. A total of 187 SSR primer pairs were designed based on SSR loci with consisting of tri- to hexa-nucleotide motifs, and 34 highly polymorphic primer pairs were selected to analyze the diversity of 231 germplasm resources. These primers amplified 219 alleles (Na) across 231 accessions, averaging of 6.441 alleles for per marker. The polymorphic information content (PIC) values ranged from 0.509 to 0.757 with a mean of 0.620. Genetic diversity varied among populations, with Southeast Asia showing the highest diversity, and Australia the lowest. Population structure analysis, divided the accessions into two groups, Group I (India) and Group II (Southeast Asia), containing 104 and 127 accessions, respectively, consistent with results from phylogenetic analysis and principal component analysis (PCA). Sixteen SSR primer pairs capable of distinguishing all tested accessions, were selected as core primers for constructing fingerprints of 229 mango accessions. These findings offer valuable resources for enhancing the utilization of mango germplasm in breeding programs.
Availability of the Great Saphenous Veins as Conduits for Arterial Bypass Surgery in Patients with Varicose Veins
Authors: Golovina Veronika , Panfilov Vladislav , Seliverstov Evgenii , Erechkanova Darina , Zolotukhin Igor
Published: December 2024

Background: The great saphenous vein (GSV) has long been recognized as the best conduit for vascular bypass procedures. Concomitant varicose veins disease may be a reason for GSV unavailability either due to dilatation and tortuosity of the vein or due to its destruction during invasive venous treatment. Objectives—to assess the rate of varicose vein patients with concomitant lower extremity arterial disease (LEAD) who have previously lost their GSV due to venous ablation. Material and Methods: A total of 285 patients (76 F, 209 M) with LEAD were consecutively enrolled. A total of 111 patients (222 limbs) underwent a detailed duplex ultrasound of the lower extremity veins for assessing suitability of the GSV as a conduit. We registered presence of varicose veins (VVs), type of previous invasive procedure and availability of saphenous veins as possible grafts. Results: The mean age of screened patients was 70.5 ± 9.1.62 (21.75%) patients had varicose veins or were operated on before due to varicose veins. A total of 42 patients with varicose veins had C2 disease, 10 had C3, 9 had C4 and 1 had C6 according to CEAP classification. A total of 222 lower extremities were examined by duplex ultrasound of which 51 limbs had VVs. Despite the presence of varicose tributaries, the GSV was suitable for bypass in 9 of those lower extremities. The GSV was not available as a conduit in 34 (19.9%) ipsilateral lower extremities in the LEAD with no VVs group and in 42 (82.6%) ipsilateral lower extremities in the LEAD with VVs group (p = 0.0001). Varicose vein disease was associated with a higher frequency of the GSV unavailability (odds ratio 18.8, 95% confidence interval 8.35–42.35). On the 11 ipsilateral limbs (5% of LEAD patients and 21.6% of LEAD with VVs patients), the GSV was unavailable due to previous venous interventions. Conclusions: Almost 20% of patients may have both LEAD and VVs. Among those with VVs, most have the ipsilateral GSV unavailable as a potential conduit. Additionally, one fifth of limbs with VVs had GSVs destroyed previously due to saphenous ablative procedures.
Fetal Isolated Single Umbilical Artery (ISUA) and Its Role as a Marker of Adverse Perinatal Outcomes
Authors: Cubo Ana María , Moreno Alicia , Sánchez-Barba Mercedes , Cabrero María Ángeles , Costas Tatiana , Rodríguez María O , Hernández Hernández María Estrella , Ordás Polán , Villalba Yarza Ana , Goenaga Francisco Javier , Lapresa-Alcalde María Victoria
Published: December 2024

Single umbilical artery (SUA) is considered an ultrasound marker of anomalies. Although it may be present in about 0.5% to 6% of normal pregnancies, it has been linked with an increased risk of fetal growth restriction (FGR), as well as cardiac, genitourinary and gastrointestinal malformations and chromosomal anomalies such as trisomies 21 and 18. Objectives: This study aims to evaluate whether the presence of isolated SUA (ISUA) is associated with adverse perinatal outcomes. Methods: A descriptive, observational and retrospective study was conducted, analyzing 1234 pregnancies (1157 normal gestations with a three-vessel cord and 77 cases of ISUA). Results: ISUA was associated with a lower gestational age (38 vs. 39 weeks) and a lower birth weight (3013 vs. 3183 g) when performing a univariate analysis. However, after performing a multivariate analysis adjusted for maternal age and BMI, the association between single umbilical artery (SUA) and lower birth weight could not be proven. No significant differences were found in the rate of malformations, genetic disorders, Apgar score, pH at birth or admissions in the neonatal ICU. Conclusions: ISUA is associated with a lower birth weight but does not increase the risk of prematurity or low-birth-weight-related neonatal admissions. Additionally, ISUA is not significantly associated with a lower gestational age, genetic disorders, fetal malformations, worse Apgar scores or lower pH values at birth.
A Novel Therapeutic Approach Targeting Spinal Accessory and Dorsal Scapular Nerves for the Relief of Posterior Neck, Trapezius, and Interscapular Pain
Authors: Park Sin-Hye , Kim Sin-Hwe , Kim Minha , Jung Jong Burm , Choi Kwangwoon , Lee Daewook , Lee Je-Hun , Seong Jeong Won , Yang Cheol-Jung
Published: December 2024

Background/Objectives: Posterior neck, trapezius, and interscapular pain, exacerbated by poor posture such as forward head and rounded shoulders, is common. In this study, we aimed to assess the clinical outcomes of isotonic saline injections at nerve entrapment points (NEPs) within the sternocleidomastoid (SCM) and scalenus medius (SM) muscles for alleviating spinal accessory nerve (SAN) and dorsal scapular nerve (DSN) compression in patients suffering from posterior neck, trapezius, and interscapular pain. Methods: In this retrospective study, 68 patients were included, with 34 receiving isotonic saline injections and 34 undergoing Extracorporeal Shock Wave Therapy (ESWT) as a control. The clinical outcomes were evaluated using the Visual Analog Scale (VAS) and Percent Pain Intensity Difference (PPID) before and after therapy. The effectiveness of isotonic saline injections targeting NEPs in the SCM and SM muscles in relieving pain associated with SAN and DSN entrapment was assessed. Results: Both treatments significantly reduced VAS and PPID scores, with injection therapy showing a larger treatment effect size (Cohen’s d: 3.521 for VAS and 3.521 for PPID) compared to ESWT (Cohen’s d: 1.379 for VAS and 1.710 for PPID). The mean clinically important difference observed for VAS was 4.2, exceeding the expected value of 2.6, indicating a substantial improvement in pain and patient quality of life. Conclusions: Isotonic saline injections at the NEPs of SAN and DSN within the SCM and SM muscles might potentially reduce posterior neck, trapezius, and interscapular pain associated with possible nerve entrapment, without causing complications. Further research is needed to validate these findings in larger, controlled trials.
Surgical Synergy in Primary Open-Angle Glaucoma: Assessing Safety and Efficacy of Hydrus, iStent, and Gonioscopy-Assisted Transluminal Trabeculotomy in Glaucoma Management
Authors: Ayoub Mohammad Zeyad Mohammad , Al-Nahrawy Ahmed
Published: December 2024

Background/Objectives: This paper will compare the outcomes—safety and efficacy—of three minimally invasive glaucoma surgeries (MIGSs),the Hydrus Microstent, iStent, and Gonioscopy-Assisted Transluminal Trabeculotomy (GATT), for intraocular pressure (IOP) reduction in patients with primary open-angle glaucoma (POAG). Methods: A literature search of Ovid Medline and Embase identified studies evaluating the Hydrus, iStent, and GATT. Data on IOP reduction, medication use, and complications were analyzed. Results: Studies show the Hydrus, iStent, and GATT reduce IOP and medication burden in POAG patients, with some complications. For the Hydrus, studies showed 37.09% (27.5 ± 4.4 to 17.3 ± 3.7 mmHg) and 25% (16.8 to 12.6 mmHg) IOP reduction. Meanwhile, medication burden decreased from 2.5 ± 0.7 to 1.0 and from 2.1 to 1.15. For the iStent, studies showed a 36.39% (21.1 to 13.4 mmHg) and 8.19% (17.1 to 15.7 mmHg) IOP drop. Medication burden decreased from 2.87 to 1.24 and from 1.7 to 0.26. For GATT, studies showed a 49.33% (27.70 ± 10.30 to 14.04 ± 3.75) and 39.09% (26.40 ± 6.37 to 16.08 ± 2.38) IOP drop. Medication burden reduced from 3.73 ± 0.98 to 1.82 ± 1.47 and from 3.12 ± 0.80 to 0.45 ± 0.96. Conclusions: The Hydrus, iStent, and GATT are effective alternatives to trabeculectomy for mild to moderate POAG. They reduce and control IOP and dependence on medications with manageable safety profiles. In all three options, there were some clinically significant complications based on the p-value. For the Hydrus, it was PAS. For the iStent, they were PAS, FB sensation, IOP spikes, and microhyphema. For GATT, it was IOP spikes. However, further long-term studies, especially randomized controlled trials, are needed to support these results.
Vestibular Hypofunction in Patients with Presbycusis: A Cross-Sectional Study
Authors: Omer Walid E. , Abdulhadi Khalid , Shahbal Saad , Neudert Marcus , Siepmann Timo
Published: December 2024

Background: Vestibular Hypofunction (VH) and hearing loss can affect quality of life and lead to disability, especially in the elderly. Studies investigating presbycusis and vestibular function in the aging population have been conducted separately, but few have examined the combination of both conditions in older patients, with inconsistent results that may be due to small sample sizes or heterogeneity in the methods used to assess vestibular function. We aimed to characterize the occurrence of VH in patients with presbycusis using the video head impulse test (vHIT), which is a specific and reliable assessment tool for VH. Methods: A prospective, cross-sectional study of 200 individuals was conducted at Hamad Medical Corporation-Qatar in Doha, Qatar. Adults aged 50 years and older with bilateral age-related hearing loss were screened for eligibility and those who were eligible completed the vHIT. Patient history, demographics, audiological and clinical data were collected. VH was defined as vestibulo-ocular reflex (VOR) gain of <0.79 and presence of corrective saccades. Results: In our study population, (n = 200 mean age 64.1 ± 8.4 [Range = 37], 31.5% females), VH was identified in 12 out of 200 patients (6.0%, 95% CI: 3.3–10.1) with bilateral age-related hearing loss (ARHL), equivalent to approximately 1 in 17 patients. VH was detected in the left ear in 11 patients (5.5%, 95% CI: 2.9–9.9), the right ear in five patients (2.5%, 95% CI: 0.8–5.8), and bilaterally in four patients (2.0%, 95% CI: 0.6–5.4). Conclusions: In our study population, vestibular hypofunction was observed in 6% of patients with bilateral symmetrical age-related sensorineural hearing loss, suggesting that vestibular screening may be useful in this population at risk.