bluuebunny/crossref_metadata_2025 huggingface dataset:
Emulsion-Based Gel Loaded with Ibuprofen and Its Derivatives
Authors: Agboola Adebukola Abiola , Nowak Anna , Duchnik Wiktoria , Kucharski Łukasz , Story Anna , Story Grzegorz , Struk Łukasz , Antosik Adrian Krzysztof , Ossowicz-Rupniewska Paula
Published: May 2023

The aim of this study was to evaluate the effect of vehicle and chemical modifications of the structure of active compounds on the skin permeation and accumulation of ibuprofen (IBU). As a result, semi-solid formulations in the form of an emulsion-based gel loaded with ibuprofen and its derivatives, such as sodium ibuprofenate (IBUNa) and L-phenylalanine ethyl ester ibuprofenate ([PheOEt][IBU]), were developed. The properties of the obtained formulations were examined, including density, refractive index, viscosity, and particle size distribution. The parameters of release and permeability through the pig skin of the active substances contained in the obtained semi-solid formulations were determined. The results indicate that an emulsion-based gel enhanced the skin penetration of IBU and its derivatives compared to two commercial preparations in the form of a gel and a cream. The average cumulative mass of IBU after a 24 h permeation test from an emulsion-based gel formulation through human skin was 1.6–4.0 times higher than for the commercial products. Ibuprofen derivatives were evaluated as chemical penetration enhancers. After 24 h of penetration, the cumulative mass was 1086.6 ± 245.8 for IBUNa and 948.6 ± 87.5 µg IBU/cm2 for [PheOEt][IBU], respectively. This study demonstrates the perspective of the transdermal emulsion-based gel vehicle in conjunction with the modification of the drug as a potentially faster drug delivery system.
Sucrose Esters as Oleogelators in Mono or Binary Structured Oleogels Using Different Oleogelation Routes
Authors: da Silva Thais Lomonaco Teodoro , Danthine Sabine
Published: May 2023

Sucrose esters (SE) have been investigated as structuring agents in oleogels. Due to the low structuration power of SE as single agent, this component has recently been explored in combination with other oleogelators to form multicomponent systems. This study aimed to evaluate binary blends of SEs with different hydrophilic-lipophilic balances (HLBs) with lecithin (LE), monoglycerides (MGs) and hard-fat (HF), according to their physical properties. The following SEs, SP10-HLB2, SP30-HLB6, SP50-HLB11, and SP70-HLB15, were structured using three different routes: “traditional”, “ethanol” and “foam-template”. All binary blends were made using a 10% oleogelator in 1:1 proportion for binary mixtures; they were then evaluated for their microstructure, melting behavior, mechanical properties, polymorphism and oil-binding capacity. SP10 and SP30 did not form well-structure and self-standing oleogels in any combination. Although SP50 showed some potential blends with HF and MG, their combination with SP70 led to even more well-structured oleogels, with a higher hardness (~0.8 N) and viscoelasticity (160 kPa), and 100% oil-binding capacity. This positive result might be attributed to the reinforcement of the H-bond between the foam and the oil by MG and HF.
Numerical Optimization of Prednisolone–Tacrolimus Loaded Ultraflexible Transethosomes for Transdermal Delivery Enhancement; Box–Behnken Design, Evaluation, Optimization, and Pharmacokinetic Study
Authors: Alfadhel Munerah M. , Zaki Randa Mohammed , Aldosari Basmah Nasser , Sayed Ossama M.
Published: May 2023

The aim of the present study is to formulate highly permeable carriers (i.e., transethosomes) for enhancing the delivery of prednisolone combined with tacrolimus for both topical and systemic pathological conditions. A Box–Behnken experimental design was implemented in this research. Three independent variables: surfactant concentration (X1), ethanol concentration (X2), and tacrolimus concentration (X3) were adopted in the design while three responses: entrapment efficiency (Y1), vesicle size (Y2), and zeta potential (Y3) were investigated. By applying design analysis, one optimum formulation was chosen to be incorporated into topical gel formulation. The optimized transethosomal gel formula was characterized in terms of pH, drug content, and spreadability. The gel formula was challenged in terms of its anti-inflammatory effect and pharmacokinetics against oral prednisolone suspension and topical prednisolone–tacrolimus gel. The optimized transethosomal gel achieved the highest rate of rat hind paw edema reduction (98.34%) and highest pharmacokinetics parameters (Cmax 133.266 ± 6.469 µg/mL; AUC0-∞ 538.922 ± 49.052 µg·h/mL), which indicated better performance of the formulated gel.
Short-Term Conservation of Juglans regia L. via Synthetic Seed Technology
Authors: Sota Valbona , Benelli Carla , Myrselaj Matilda , Kongjika Efigjeni , Gruda Nazim S.
Published: May 2023

Juglans regia L. is a crucial species as a forest tree and for its nutritional and medicinal values. It is also included in the list of endangered species in Albania and thus, there is a need to find methodologies to ensure its rapid regeneration and ex situ conservation. This research, investigated the regeneration of plantlets from synthetic seeds containing shoot tips of four native walnut varieties: ‘Përmet’, ‘Korçë’, ‘Peshkopi’, and ‘Tropojë’. First, in vitro-derived shoot tips from walnut seedlings are encapsulated using sodium alginate. After that, the regeneration potential of the encapsulated shoot tips and the influence of incubation conditions are evaluated. The synthetic seeds were incubated at either 25 °C or 8 °C, with and without dehydration treatment, in 0.5 M sucrose solution for 3 h. The synthetic seeds in both temperature regimes (25 °C and 8 °C) develop plantlets and provid conservation potential without the need for subcultures for 4 and 3.5 months, respectively. Furthermore, all walnut varieties incubated in these conditions achiev a high regeneration rates.
Effects of Cold Plasma and Ozone Water Treatment on Micronutrient Solubility
Authors: Thakulla Dharti , Fisher Paul R.
Published: May 2023

Cold plasma and ozone sanitation of irrigation solutions can oxidize both microbes and non-target micronutrients because their high oxidation-reduction potential (ORP) is a non-selective mode of action. The objective of this study was to evaluate the effects of cold plasma and ozone treatment on oxidation of iron and manganese in nutrient solutions containing one of four iron chelates (iron-ethylenediaminetetraacetic acid (Fe-EDTA), iron-diethylenetriaminepentaacetic acid (Fe-DTPA), iron-ethylenediamine-N,N′-bis(2-hydroxyphenylacetic acid) (Fe-EDDHA), and hydroxybenzyl ethylenediamine (Fe-HBED)). Nutrient solutions were recirculated through the cold plasma or ozone system until the ORP reached 700 mV. The concentrations of total dissolved iron, manganese, and chelated iron were measured before and after passing through the treatment systems. Both cold plasma and ozone oxidized chelates and decreased the solubility of iron and manganese. Cold plasma and ozone had similar effects on micronutrients, pH, electrical conductivity, and dissolved oxygen at a standardized target ORP of 700 mV. Fe-EDTA was the most resistant chelate to oxidation. With Fe-EDTA, ORP increased more quickly, and the concentration of chelated Fe decreased less with the increasing ORP over time compared with Fe-DTPA, Fe-EDDHA, and Fe-HBED. The concentration of chelated Fe decreased by up to 80% for EDDHA at 700 mV compared with a 20% decrease for EDTA. The concentration of Mn decreased by up to 85% at 700 mV. The design of water treatment with cold plasma or ozone therefore requires consideration of secondary effects on micronutrients. The treatment dosage, flow rate, and nutrient solution at a particular grower operation are likely to affect the quantity of micronutrient fertilizer that needs to be supplemented following treatment. Use of Fe-EDTA is one strategy to reduce the loss of iron and increase residual ORP that is available for sanitation.
Partnering with First Nations in Northern British Columbia Canada to Reduce Inequity in Access to Genomic Research
Authors: Caron Nadine R. , Adam Wilf , Anderson Kate , Boswell Brooke T. , Chongo Meck , Deineko Viktor , Dick Alexanne , Hall Shannon E. , Hatcher Jessica T. , Howard Patricia , Hunt Megan , Linn Kevin , O’Neill Ashling
Published: May 2023

Indigenous-led, culturally safe health research and infrastructure are essential to address existing inequities and disparities for Indigenous Peoples globally. Biobanking, genomic research, and self-governance could reduce the existing divide and increase Indigenous participation in health research. While genomic research advances medicine, barriers persist for Indigenous patients to benefit. In northern BC, Canada, the Northern Biobank Initiative (NBI), with guidance from a Northern First Nations Biobank Advisory Committee (NFNBAC), has engaged in consultations with First Nations on biobanking and genomic research. Key informant interviews and focus groups conducted with First Nations leaders, Elders, Knowledge Keepers, and community members established culturally safe ways of biobanking and exploring genomic research. Strong support for a Northern British Columbia First Nations Biobank (NBCFNB) that will promote choice, inclusion, and access to health research opportunities emerged. The acceptance and enthusiasm for the development of this NBCFNB and its governance table highlight the shift towards Indigenous ownership and support of health research and its benefits. With engagement and partnership, community awareness, multigenerational involvement, and support from diverse and experienced healthcare leaders, the NBCFNB will establish this culturally safe, locally driven, and critically important research priority that may serve as an example for diverse Indigenous groups when designing their unique biobanking or genomic research opportunities.
Dribble Accuracy and Arm Coordination Pattern According to Motor Expertise and Tempo
Authors: Park Jinhan , Jeong Jaeuk
Published: May 2023

Skilled movements in motor learning result from efficiently controlling the many degrees of freedom in human movement. To acquire motor skills, harmonious coordination of body segments in time and space is crucial for accurate and consistent performance. The purpose of this study was to compare dribbling accuracy, consistency, and coordination patterns of body segments according to motor expertise and tempo. To achieve this, we had eight basketball experts and eight beginners perform static dribbling at three different speeds for 20 s. Force plates measured radial error while motion capture equipment measured the angular data of the right arm’s fingers, wrist, and elbow. The measurements obtained from the force plate were used to analyze the participants’ dribbling performance, including accuracy, consistency, and coordination patterns. The research results showed that there was no significant difference in dribbling accuracy according to skill level, but skilled players showed higher consistency in the anterior–posterior (AP) direction (p < 0.001). In the comparative analysis of coordination patterns, skilled players showed an in-phase structure, whereas beginners showed an anti-phase structure (elbow–wrist: p < 0.05; wrist–finger: p < 0.001; elbow–finger: p < 0.001). This study suggests that achieving proficiency in basketball dribbling requires a strategy that involves coordination of movements with an in-phase pattern for stability in performance.
Cold-Adapted Proteases: An Efficient and Energy-Saving Biocatalyst
Authors: Yang Zhengfeng , Huang Zhendi , Wu Qian , Tang Xianghua , Huang Zunxi
Published: May 2023

The modern biotechnology industry has a demand for macromolecules that can function in extreme environments. One example is cold-adapted proteases, possessing advantages such as maintaining high catalytic efficiency at low temperature and low energy input during production and inactivation. Meanwhile, cold-adapted proteases are characterised by sustainability, environmental protection, and energy conservation; therefore, they hold significant economic and ecological value regarding resource utilisation and the global biogeochemical cycle. Recently, the development and application of cold-adapted proteases have gained gaining increasing attention; however, their applications potential has not yet been fully developed, which has seriously restricted the promotion and application of cold-adapted proteases in the industry. This article introduces the source, related enzymology characteristics, cold resistance mechanism, and the structure-function relationship of cold-adapted proteases in detail. This is in addition to discussing related biotechnologies to improve stability, emphasise application potential in clinical medical research, and the constraints of the further developing of cold-adapted proteases. This article provides a reference for future research and the development of cold-adapted proteases.
Identifying the Soybean microRNAs Related to Phytophthora sojae Based on RNA Sequencing and Bioinformatics Analysis
Authors: Zhang Zhanguo , Jin Song , Tian Huilin , Wang Zhihao , Jiang Rui , Liu Chunyan , Xin Dawei , Wu Xiaoxia , Chen Qingshan , Zhu Rongsheng
Published: May 2023

Phytophthora root rot in soybeans is caused by a pathogen called Phytophthora sojae (P. sojae), which results in a significant decrease in soybean production within affected regions. MicroRNAs (miRNAs) are a class of small non-coding RNA molecules that play a key post-transcriptional regulatory role in eukaryotes. In this paper, the miRNAs that respond to P. sojae were analyzed from the gene level to complement the study of molecular resistance mechanisms in soybean. The study utilized high-throughput sequencing of soybean data to predict miRNAs that respond to P. sojae, analyze their specific functions, and verify regulatory relationships using qRT-PCR. The results showed that the miRNAs in soybean respond to P. sojae infection. MiRNAs can be transcribed independently, suggesting the presence of transcription factor binding sites in the promoter regions. Additionally, we performed an evolutionary analysis on conserved miRNAs that respond to P. sojae. Finally, we investigated the regulatory relationships among miRNAs, genes, and transcription factors, and identified five regulatory patterns. These findings lay the groundwork for future studies on the evolution of miRNAs responsive to P. sojae.
Evidence for Angiotensin II as a Naturally Existing Suppressor for the Guanylyl Cyclase A Receptor and Cyclic GMP Generation
Authors: Ma Xiao , Iyer Seethalakshmi R. , Ma Xiaoyu , Reginauld Shawn H. , Chen Yang , Pan Shuchong , Zheng Ye , Moroni Dante G. , Yu Yue , Zhang Lianwen , Cannone Valentina , Chen Horng H. , Ferrario Carlos M. , Sangaralingham S. Jeson , Burnett John C.
Published: May 2023

The natriuretic peptide system (NPS) and renin-angiotensin-aldosterone system (RAAS) function oppositely at multiple levels. While it has long been suspected that angiotensin II (ANGII) may directly suppress NPS activity, no clear evidence to date supports this notion. This study was designed to systematically investigate ANGII–NPS interaction in humans, in vivo, and in vitro. Circulating atrial, b-type, and c-type natriuretic peptides (ANP, BNP, CNP), cyclic guanosine monophosphate (cGMP), and ANGII were simultaneously investigated in 128 human subjects. Prompted hypothesis was validated in vivo to determine the influence of ANGII on ANP actions. The underlying mechanisms were further explored via in vitro approaches. In humans, ANGII demonstrated an inverse relationship with ANP, BNP, and cGMP. In regression models predicting cGMP, adding ANGII levels and the interaction term between ANGII and natriuretic peptides increased the predictive accuracy of the base models constructed with either ANP or BNP, but not CNP. Importantly, stratified correlation analysis further revealed a positive association between cGMP and ANP or BNP only in subjects with low, but not high, ANGII levels. In rats, co-infusion of ANGII even at a physiological dose attenuated cGMP generation mediated by ANP infusion. In vitro, we found the suppressive effect of ANGII on ANP-stimulated cGMP requires the presence of ANGII type-1 (AT1) receptor and mechanistically involves protein kinase C (PKC), as this suppression can be substantially rescued by either valsartan (AT1 blocker) or Go6983 (PKC inhibitor). Using surface plasmon resonance (SPR), we showed ANGII has low binding affinity to the guanylyl cyclase A (GC-A) receptor compared to ANP or BNP. Our study reveals ANGII is a natural suppressor for the cGMP-generating action of GC-A via AT1/PKC dependent manner and highlights the importance of dual-targeting RAAS and NPS in maximizing beneficial properties of natriuretic peptides in cardiovascular protection.
Inflammatory Response in COVID-19 Depending on the Severity of the Disease and the Vaccination Status
Authors: Trofin Felicia , Nastase Eduard Vasile , Roșu Manuel Florin , Bădescu Aida Corina , Buzilă Elena Roxana , Miftode Egidia Gabriela , Manciuc Doina Carmen , Dorneanu Olivia Simona
Published: May 2023

The aim of this study was to analyze the serum concentration of interleukin-6 (IL-6), C-reactive protein (CRP), D-dimer, lactate dehydrogenase (LDH), ferritin, and procalcitonin in COVID-19 patients with different forms of the disease. We performed a prospective cohort study on 137 COVID-19 consecutive patients, divided into four groups according to the severity of the disease as follows: 30 patients in the mild form group, 49 in the moderate form group, 28 in the severe form group, and 30 in the critical form group. The tested parameters were correlated with COVID-19 severity. Significant differences were registered between the form of COVID-19 depending on the vaccination status, between LDH concentrations depending on the virus variant, and in IL-6, CRP, and ferritin concentrations and vaccination status depending on the gender. ROC analysis revealed that D-dimer best predicted COVID-19 severe forms and LDH predicted the virus variant. Our findings confirmed the interdependence relationships observed between inflammation markers in relation to the clinical severity of COVID-19, with all the tested biomarkers increasing in severe and critical COVID-19. IL-6, CRP, ferritin, LDH, and D-dimer were increased in all COVID-19 forms. These inflammatory markers were lower in Omicron-infected patients. The unvaccinated patients developed more severe forms compared to the vaccinated ones, and a higher proportion of them needed hospitalization. D-dimer could predict a severe form of COVID-19, while LDH could predict the virus variant.
Secondary Osteoporosis: A Still Neglected Condition
Authors: Favero Vittoria , Eller-Vainicher Cristina , Chiodini Iacopo
Published: May 2023

The condition of “secondary osteoporosis” is defined as a bone loss that results from specific well-defined clinical disorders [...]
Probiotic Yoghurt Enriched with Mango Peel Powder: Biotransformation of Phenolics and Modulation of Metabolomic Outputs after In Vitro Digestion and Colonic Fermentation
Authors: Zahid Hafza Fasiha , Ali Akhtar , Legione Alistair R. , Ranadheera Chaminda Senaka , Fang Zhongxiang , Dunshea Frank R. , Ajlouni Said
Published: May 2023

This study investigated the health-promoting effects and prebiotic functions of mango peel powder (MPP) both as a plain individual ingredient and when incorporated in yoghurt during simulated digestion and fermentation. The treatments included plain MPP, plain yoghurt (YA), yoghurt fortified with MPP (YB), and yoghurt fortified with MPP and lactic acid bacteria (YC), along with a blank (BL). The identification of polyphenols in the extracts of insoluble digesta and phenolic metabolites after the in vitro colonic fermentation were performed employing LC-ESI-QTOF-MS2. These extracts were also subjected to pH, microbial count, production of SCFA, and 16S rRNA analyses. The characterisation of phenolic profiles identified 62 phenolic compounds. Among these compounds, phenolic acids were the major compounds that underwent biotransformation via catabolic pathways such as ring fission, decarboxylation, and dehydroxylation. Changes in pH indicated that YC and MPP reduced the media pH from 6.27 and 6.33 to 4.50 and 4.53, respectively. This decline in pH was associated with significant increases in the LAB counts of these samples. The Bifidobacteria counts were 8.11 ± 0.89 and 8.02 ± 1.01 log CFU/g in YC and MPP, respectively, after 72 h of colonic fermentation. Results also showed that the presence of MPP imparted significant variations in the contents and profiles of individual short chain fatty acids (SCFA) with more predominant production of most SCFA in the MPP and YC treatments. The 16s rRNA sequencing data indicated a highly distinctive microbial population associated with YC in terms of relative abundance. These findings suggested MPP as a promising ingredient for utilisation in functional food formulations aiming to enhance gut health.
Anti-Inflammatory Effects of Adiponectin Receptor Agonist AdipoRon against Intervertebral Disc Degeneration
Authors: Ohnishi Hiroki , Zhang Zhongying , Yurube Takashi , Takeoka Yoshiki , Kanda Yutaro , Tsujimoto Ryu , Miyazaki Kunihiko , Matsuo Tomoya , Ryu Masao , Kumagai Naotoshi , Kuroshima Kohei , Hiranaka Yoshiaki , Kuroda Ryosuke , Kakutani Kenichiro
Published: May 2023

Adiponectin, a hormone secreted by adipocytes, has anti-inflammatory effects and is involved in various physiological and pathological processes such as obesity, inflammatory diseases, and cartilage diseases. However, the function of adiponectin in intervertebral disc (IVD) degeneration is not well understood. This study aimed to elucidate the effects of AdipoRon, an agonist of adiponectin receptor, on human IVD nucleus pulposus (NP) cells, using a three-dimensional in vitro culturing system. This study also aimed to elucidate the effects of AdipoRon on rat tail IVD tissues using an in vivo puncture-induced IVD degeneration model. Analysis using quantitative polymerase chain reaction demonstrated the downregulation of gene expression of proinflammatory and catabolic factors by interleukin (IL)-1β (10 ng/mL) in human IVD NP cells treated with AdipoRon (2 μM). Furthermore, western blotting showed AdipoRon-induced suppression of p65 phosphorylation (p < 0.01) under IL-1β stimulation in the adenosine monophosphate-activated protein kinase (AMPK) pathway. Intradiscal administration of AdipoRon was effective in alleviating the radiologic height loss induced by annular puncture of rat tail IVD, histomorphological degeneration, production of extracellular matrix catabolic factors, and expression of proinflammatory cytokines. Therefore, AdipoRon could be a new therapeutic candidate for alleviating the early stage of IVD degeneration.
Identification of New FG-Repeat Nucleoporins with Amyloid Properties
Authors: Danilov Lavrentii G. , Sukhanova Xenia V. , Rogoza Tatiana M. , Antonova Ekaterina Y. , Trubitsina Nina P. , Zhouravleva Galina A. , Bondarev Stanislav A.
Published: May 2023

Amyloids are fibrillar protein aggregates with a cross-β structure. More than two hundred different proteins with amyloid or amyloid-like properties are already known. Functional amyloids with conservative amyloidogenic regions were found in different organisms. Protein aggregation appears to be beneficial for the organism in these cases. Therefore, this property might be conservative for orthologous proteins. The amyloid aggregates of the CPEB protein were suggested to play an important role in the long-term memory formation in Aplysia californica, Drosophila melanogaster, and Mus musculus. Moreover, the FXR1 protein demonstrates amyloid properties among the Vertebrates. A few nucleoporins (e.g., yeast Nup49, Nup100, Nup116, and human Nup153 and Nup58), are supposed or proved to form amyloid fibrils. In this study, we performed wide-scale bioinformatic analysis of nucleoporins with FG-repeats (phenylalanine–glycine repeats). We demonstrated that most of the barrier nucleoporins possess potential amyloidogenic properties. Furthermore, the aggregation-prone properties of several Nsp1 and Nup100 orthologs in bacteria and yeast cells were analyzed. Only two new nucleoporins, Drosophila melanogaster Nup98 and Schizosaccharomyces pombe Nup98, aggregated in different experiments. At the same time, Taeniopygia guttata Nup58 only formed amyloids in bacterial cells. These results rather contradict the hypothesis about the functional aggregation of nucleoporins.
The Role of Steroidomics in the Diagnosis of Alzheimer’s Disease and Type 2 Diabetes Mellitus
Authors: Vaňková Markéta , Velíková Marta , Vejražková Daniela , Včelák Josef , Lukášová Petra , Rusina Robert , Vaňková Hana , Jarolímová Eva , Kancheva Radmila , Bulant Josef , Horáčková Lenka , Bendlová Běla , Hill Martin
Published: May 2023

Epidemiological studies suggest an association between Alzheimer’s disease (AD) and type 2 diabetes mellitus (T2DM). This study aimed to investigate the pathophysiological markers of AD vs. T2DM for each sex separately and propose models that would distinguish control, AD, T2DM, and AD-T2DM comorbidity groups. AD and T2DM differed in levels of some circulating steroids (measured mostly by GC-MS) and in other observed characteristics, such as markers of obesity, glucose metabolism, and liver function tests. Regarding steroid metabolism, AD patients (both sexes) had significantly higher sex hormone binding globulin (SHBG), cortisol, and 17-hydroxy progesterone, and lower estradiol and 5α-androstane-3α,17β-diol, compared to T2DM patients. However, compared to healthy controls, changes in the steroid spectrum (especially increases in levels of steroids from the C21 group, including their 5α/β-reduced forms, androstenedione, etc.) were similar in patients with AD and patients with T2DM, though more expressed in diabetics. It can be assumed that many of these steroids are involved in counter-regulatory protective mechanisms that mitigate the development and progression of AD and T2DM. In conclusion, our results demonstrated the ability to effectively differentiate AD, T2DM, and controls in both men and women, distinguish the two pathologies from each other, and differentiate patients with AD and T2DM comorbidities.
COVID-19—The Shift of Homeostasis into Oncopathology or Chronic Fibrosis in Terms of Female Reproductive System Involvement
Authors: Petersen Elena , Chudakova Daria , Erdyneeva Daiana , Zorigt Dulamsuren , Shabalina Evgeniya , Gudkov Denis , Karalkin Pavel , Reshetov Igor , Mynbaev Ospan A.
Published: May 2023

The COVID-19 pandemic caused by the SARS-CoV-2 coronavirus remains a global public health concern due to the systemic nature of the infection and its long-term consequences, many of which remain to be elucidated. SARS-CoV-2 targets endothelial cells and blood vessels, altering the tissue microenvironment, its secretion, immune-cell subpopulations, the extracellular matrix, and the molecular composition and mechanical properties. The female reproductive system has high regenerative potential, but can accumulate damage, including due to SARS-CoV-2. COVID-19 is profibrotic and can change the tissue microenvironment toward an oncogenic niche. This makes COVID-19 and its consequences one of the potential regulators of a homeostasis shift toward oncopathology and fibrosis in the tissues of the female reproductive system. We are looking at SARS-CoV-2-induced changes at all levels in the female reproductive system.
A Step Forward: About the Progresses Made in the Second Edition of the Special Issue “The Multiple Mechanisms Underlying Neuropathic Pain”
Authors: Marinelli Sara , Coccurello Roberto
Published: May 2023

The present editorial intends to comment on the contributions published in the second edition of the Special Issue (SI) “The Multiple Mechanisms Underlying Neuropathic Pain” [...]
Pathophysiology and Clinical Impacts of Chronic Kidney Disease on Coronary Artery Calcification
Authors: Dai Zhuoming , Zhang Xiangyu
Published: May 2023

The global prevalence of chronic kidney disease (CKD) has increased in recent years. Adverse cardiovascular events have become the main cause of life-threatening events in patients with CKD, and vascular calcification is a risk factor for cardiovascular disease. Vascular calcification, especially coronary artery calcification, is more prevalent, severe, rapidly progressive, and harmful in patients with CKD. Some features and risk factors are unique to vascular calcification in patients with CKD; the formation of vascular calcification is not only influenced by the phenotypic transformation of vascular smooth muscle cells, but also by electrolyte and endocrine dysfunction, uremic toxin accumulation, and other novel factors. The study on the mechanism of vascular calcification in patients with renal insufficiency can provide a basis and new target for the prevention and treatment of this disease. This review aims to illustrate the impact of CKD on vascular calcification and to discuss the recent research data on the pathogenesis and factors involved in vascular calcification, mainly focusing on coronary artery calcification, in patients with CKD.
Use of CO2-Derived Variables in Cardiac Intensive Care Unit: Pathophysiology and Clinical Implications
Authors: Cousin Vladimir L. , Joye Raphael , Wacker Julie , Beghetti Maurice , Polito Angelo
Published: May 2023

Shock is a life-threatening condition, and its timely recognition is essential for adequate management. Pediatric patients with congenital heart disease admitted to a cardiac intensive care unit (CICU) after surgical corrections are particularly at risk of low cardiac output syndrome (LCOS) and shock. Blood lactate levels and venous oxygen saturation (ScVO2) are usually used as shock biomarkers to monitor the efficacy of resuscitation efforts, but they are plagued by some limitations. Carbon dioxide (CO2)-derived parameters, namely veno-arterial CO2 difference (ΔCCO2) and the VCO2/VO2 ratio, may represent a potentially valuable addition as sensitive biomarkers to assess tissue perfusion and cellular oxygenation and may represent a valuable addition in shock monitoring. These variables have been mostly studied in the adult population, with a strong association between ΔCCO2 or VCO2/VO2 ratio and mortality. In children, particularly in CICU, few studies looked at these parameters, while they reported promising results on the use of CO2-derived indices for patients’ management after cardiac surgeries. This review focuses on the physiological and pathophysiological determinants of ΔCCO2 and VCO2/VO2 ratio while summarizing the actual state of knowledge on the use of CO2-derived indices as hemodynamical markers in CICU.
The Role of Vitamin D in Reducing the Risk of Metabolic Disturbances That Cause Cardiovascular Diseases
Authors: Al-Oanzi Ziad H. , Alenazy Fawaz O. , Alhassan Hassan H. , Alruwaili Yasir , Alessa Abdulaziz I. , Alfarm Nouf B. , Alanazi Maha O. , Alghofaili Sarah I.
Published: May 2023

Among the most common problems facing public health today is a lack of vitamin D, which plays a role in the physiological processes of chronic illness conditions. Vitamin D deficiency in metabolic disorders has primary effects on osteoporosis, obesity, hypertension, diabetes, and cardiovascular disease (CVD). Vitamin D acts as a “co-hormone” in the various tissues of the body, and it has been found that vitamin D receptors (VDR) are present on all cell types, suggesting that vitamin D has a wide range of effects on most cells. Recently, there has been a surge in interest in assessing its roles. Vitamin D insufficiency increases the risk of diabetes because it lowers insulin sensitivity, and also raises the risk of obesity and CVD because of its effect on the body’s lipid profile, particularly in terms of the prevalence of dangerously high levels of low-density lipoproteins (LDL). Furthermore, vitamin D insufficiency is often related to CVD and connected risk factors, highlighting the need to know vitamin D’s functions in relation to metabolic syndrome and related processes. Through looking at previous studies, this paper explains why vitamin D is important, how deficiency is related to risk factors for metabolic syndrome through different mechanisms, and how deficiency affects CVD.
Mammography Datasets for Neural Networks—Survey
Authors: Mračko Adam , Vanovčanová Lucia , Cimrák Ivan
Published: May 2023

Deep neural networks have gained popularity in the field of mammography. Data play an integral role in training these models, as training algorithms requires a large amount of data to capture the general relationship between the model’s input and output. Open-access databases are the most accessible source of mammography data for training neural networks. Our work focuses on conducting a comprehensive survey of mammography databases that contain images with defined abnormal areas of interest. The survey includes databases such as INbreast, the Curated Breast Imaging Subset of Digital Database for Screening Mammography (CBIS-DDSM), the OPTIMAM Medical Image Database (OMI-DB), and The Mammographic Image Analysis Society Digital Mammogram Database (MIAS). Additionally, we surveyed recent studies that have utilized these databases in conjunction with neural networks and the results they have achieved. From these databases, it is possible to obtain at least 3801 unique images with 4125 described findings from approximately 1842 patients. The number of patients with important findings can be increased to approximately 14,474, depending on the type of agreement with the OPTIMAM team. Furthermore, we provide a description of the annotation process for mammography images to enhance the understanding of the information gained from these datasets.
Making the Case for Standardized Outcome Measures in Exercise and Physical Activity Research in Chronic Kidney Disease
Authors: Wilkinson Thomas J. , MacRae Jennifer M. , Thompson Stephanie , Bohm Clara
Published: May 2023

Physical activity and exercise are core components of lifestyle modification strategies for the management of chronic kidney disease (CKD). Yet, physical activity levels have consistently remained poor across all stages of CKD. Exercise interventions, including aerobic and resistance training, and lifestyle interventions promoting physical activity, have been shown to improve a multitude of clinical endpoints and factors important to patients; however, despite the evidence, the provision of physical activity in clinical practice is still inadequate. The usefulness of any study hinges on the adequacy and clinical relevance of the outcomes and outcome measures used. Inconsistent reporting and wide disparities in outcome use across studies limit evidence synthesis to help guide clinical practice. The kidney exercise and physical activity field has been particularly prone to inconsistent outcome reporting. To ensure research is relevant and able to influence clinical practice and future research, we need to ensure the use (and reporting) of standardized, relevant outcome measures. Core outcome sets (COS) have been widely developed across many chronic conditions, yet these COS have not been tailored to physical activity and exercise in CKD. Outcomes in clinical research need to be relevant to the intervention being employed. From this perspective, we summarize the importance that standardizing outcomes and outcome measures may have in relation to physical activity and exercise interventions for people living with kidney disease.
Novel Pectin Binder for Satelliting Carbides to H13 Tool Steel for PBF-LB Processing
Authors: Meyer Fabian , Kolodzy Fabian , Scheck Marie Luise , Kaletsch Anke , Kharandiuk Tetiana , Pich Andrij , Broeckmann Christoph
Published: May 2023

In order to enhance the range of processable alloys of laser-based powder bed fusion, reinforced alloys have gained focus. Satelliting is a recently introduced method for adding fine additives to larger parent powder particles using a bonding agent. Satellited particles prevent a local demixing due to size and density effects of the powder. In this study, the satelliting method is used for the additivation of Cr3C2 to AISI H13 tool steel via a functional polymer binder (pectin). The investigation includes a detailed binder analysis and comparison to the previously used PVA binder as well as processability in PBF-LB and the microstructure of the alloy. The results reveal that pectin is a suitable binder for the satelliting process and the demixing behavior that appears when using a simple powder blend can be significantly reduced. However, the alloy is enriched with carbon, which results in austenite being retained. Thus, in future research, a reduced binder content will be investigated.
Control System for Automated Technological Process of Hot Stamping—A Case Study
Authors: Wróbel Ireneusz , Danielczyk Piotr
Published: May 2023

Hot forming process has been used more and more frequently in the production of body structures of modern ultralight passenger cars for several years. This, unlike the commonly used cold stamping, is a complicated process, combining heat treatment and plastic-forming methods. For this reason, permanent control at each stage is required. This includes, among others, measurement of the blank thickness, monitoring its heating process in the suitable atmosphere in the furnace, control of the forming process itself, measurement of shape-dimensional accuracy as well as mechanical parameters of the finished drawpiece. This paper discusses the method of controlling the values of production parameters during the hot stamping process of a selected drawpiece. For this purpose, digital twins of the production line and the stamping process, made in accordance with the assumptions of Industry 4.0, have been used. Individual components of the production line with sensors for monitoring process parameters have been shown. The system’s response to emerging threats has also been described. The correctness of the adopted values is confirmed via tests of mechanical properties and the assessment of the shape-dimensional accuracy of a drawpiece test series.
Particles–Matrix Bond in ZnCoO:H and ZnCoAlO:H Films: Issues of Magnetism and Spin Injection
Authors: Samoshkina Yu. E. , Rautskii M. V. , Neznakhin D. S. , Stepanova E. A. , Edelman I. S. , Chou Hsiung
Published: May 2023

ZnCoO:H and ZnCoAlO:H films were synthesized by radio frequency magnetron sputtering in a (1 − x)Ar + xH2 mixed atmosphere with x = 0.2–0.5. The films contain different amounts of metallic Co particles (from 7.6% and higher) ~4–7 nm in size. The magnetic and magneto-optical (MO) behavior of the films was analyzed in combination with their structural data. The samples exhibit high values of magnetization (up to 377 emu/cm3) and MO response at room temperature. Two situations are considered: (1) the film magnetism is associated only with isolated metal particles and (2) magnetism is present both in the oxide matrix and in metal inclusions. It has been established that the formation mechanism of the magnetic structure of ZnO:Co2+ is due to the spin-polarized conduction electrons of metal particles and zinc vacancies. It was also found that in the presence of two magnetic components in the films, these components are exchange-coupled. In this case, the exchange coupling generates a high spin polarization of the films. The spin-dependent transport properties of the samples have been studied. A high value of the negative magnetoresistance of the films at room temperature (~4%) was found. This behavior was explained in terms of the giant magnetoresistance model. Thus, the ZnCoO:H and ZnCoAlO:H films with high spin polarization can be considered as sources of spin injection.
Koch Hierarchical Honeycomb: A Fractal-Based Design for Enhanced Mechanical Performance and Energy Absorption
Authors: Zhu Yuwen , Deng Junjie , Xiong Wei , You Tianyu , Zhou Wei
Published: May 2023

A novel energy-absorbing structure, the Koch hierarchical honeycomb, which combines the Koch geometry with a conventional honeycomb structure, is proposed in this work. Adopting a hierarchical design concept using Koch has improved the novel structure more than the honeycomb. The mechanical properties of this novel structure under impact loading are studied by finite element simulation and compared with the conventional honeycomb structure. To effectively verify the reliability of the simulation analysis, quasi-static compression experiments were conducted on 3D-printed specimens. The results of the study showed that the first-order Koch hierarchical honeycomb structure increased the specific energy absorption by 27.52% compared to the conventional honeycomb structure. Furthermore, the highest specific energy absorption can be obtained by increasing the hierarchical order to 2. Moreover, the energy absorption of triangular and square hierarchies can be significantly increased. All achievements in this study provide significant guidelines in the reinforcement design of lightweight structures.
Luminescence Properties of Green Phosphor Ca2Ga2(Ge1-xSix)O7:y%Eu2+ and Application
Authors: Kong Xiangqian , Qiu Zhihua , Wu Lina , Lei Yunfei , Chi Lisheng
Published: May 2023

Rare earth luminescent materials demonstrate significant advantages in lighting and energy saving, and detection etc. In this paper, a series of Ca2Ga2(Ge1-xSix)O7:y%Eu2+ phosphors were synthesized by high-temperature solid-state reaction and characterized by X-ray diffraction and luminescence spectroscopy methods. The powder X-ray diffraction patterns reveal that all the phosphors are isostructural with a space group of P4¯21m. The excitation spectra of Ca2Ga2(Ge1-xSix)O7:1%Eu2+ phosphors exhibit significant overlapping of the host and the Eu2+ absorption bands, which facilitates Eu2+ absorbing the energy to increase its luminescence efficiency when excited by visible photons. The emission spectra show that the Eu2+ doped phosphors have a broad emission band with a peak centered at 510 nm arising from the 4f65d1→4f7 transition. Variable temperature fluorescence reveals that the phosphor has a strong luminescence at low temperature but has a severe thermal quenching effect when temperature rises. The optimal Ca2Ga2(Ge0.5Si0.5)O7:1.0%Eu2+ phosphor shows promise for application in the field of fingerprint identification based on the experimental results.
Epidemiology and Outcome of Acute Appendicitis during and before the COVID-19 Pandemic: A Retrospective Single-Center Analysis
Authors: Won Moonho , Ahn Chiwon
Published: May 2023

Background and Objectives: We investigated epidemiological factors and outcomes, including the development of complications, for patients with appendicitis according to three sequential coronavirus disease 2019 (COVID-19) pandemic periods, divided by specific time points. Materials and Methods: This observational study included patients with acute appendicitis who arrived at a single-center between March 2019 and April 2022. The study divided the pandemic into three periods: period A as the first phase of the pandemic (from 1 March 2020 to 22 August 2021), period B as the time period the medical system stabilized (from 23 August 2021 to 31 December 2021), and period C as the time period of the exploration of patients with COVID-19 in South Korea (from 1 January 2022 to 30 April 2022). Data collection was based on medical records. The primary outcome was presence or absence of complications and the secondary outcomes were the time taken from ED visit to surgical intervention, the presence and time of the first administration of antibiotics, and the hospital stay time. Results: Of 1,101 patients, 1,039 were included, with 326 and 711 patients before and during the pandemic, respectively. Incidence of complications was not affected during the pandemic (before the pandemic 58.0%; period A 62.7%; period B,55.4%; and period C 58.1%; p = 0.358). Time from symptom onset to emergency department (ED) arrival significantly decreased during the pandemic (before the pandemic 47.8 ± 84.3 h; pandemic 35.0 ± 54 h; p = 0.003). Time from ED visit to the operating room was statistically significantly increased during the pandemic (before the pandemic 14.3 ± 21.67 h; period A 18.8 ± 14.02 h; period B 18.8 ± 8.57 h; period C 18.3 ± 12.95 h; p = 0.001). Age and time from symptom onset to ED arrival were variables affecting the incidence of complications; however, they were not affected during the pandemic (age, OR 2.382; 95% CI 1.545–3.670; time from symptom onset to ED arrival, OR 1.010, 95% CI 1.006–1.010; p < 0.001). Conclusions: This study found no differences in postoperative complications or treatment durations between pandemic periods. The incidence of appendicitis complications was significantly influenced by age and the duration between the onset of symptoms and arrival at the emergency department, but not by the pandemic period itself.
Optimisation of Imaging Confocal Microscopy for Topography Measurements of Metal Additive Surfaces
Authors: Newton Lewis , Thanki Aditi , Bermudez Carlos , Artigas Roger , Thompson Adam , Haitjema Han , Leach Richard
Published: May 2023

Additive manufactured surfaces, especially metal powder bed fusion surfaces, present unique challenges for measurement because of their complex topographies. To address these measurement challenges, optimisation of the measurement process is required. Using a statistical approach, sensitivity analyses were performed on measurement settings found on a commercial programmable array scanning confocal microscope. The instrument measurement process parameters were compared by their effects on three quality indicators: the areal surface texture parameter Sa, measurement noise, and number of non-measured points. An analysis was performed using a full factorial design of experiments for both the top and side surfaces of test surfaces made from Inconel 718 and Ti-6Al-4V using powder bed fusion. The results indicated that measurements of metal additive surfaces are robust to changes in the measurement control parameters for Sa, with variations within 5% of the mean parameter value for the same objective, surface, and measured area. The number of non-measured points and the measurement noise were more varied and were affected by the choice of measurement control parameters, but such changes could be predicted by the statistical models. The contribution offered by this work is an increased understanding of imaging confocal microscopy measurement of metal additive surfaces, along with the establishment of good practice guidance for measurements.
Radiation Exposure in Endovascular Surgery According to Complexity: Protocol for a Prospective Observational Study
Authors: Martinez Del Carmen Dorelly Tanayra , Saldaña Gutierrez Pablo , Vila Coll Ramon , Iborra Ortega Elena
Published: May 2023

In the past decades, we have witnessed tremendous developments in endovascular surgery. Nowadays, highly complex procedures are performed by minimally invasive means. A key point is equipment improvement. Modern C-arms provide advanced imaging capabilities, facilitating endovascular navigation with an adequate open surgical environment. Nevertheless, radiation exposure remains an issue of concern. This study aims to analyze radiation used during endovascular procedures according to complexity, comparing a mobile X-ray system with a hybrid room (fixed X-ray system). This is an observational and prospective study based on a cohort of non-randomized patients treated by endovascular procedures in a Vascular Surgery department using two imaging systems. The study is planned for a 3-year duration with a recruitment period of 30 months (beginning 20 July 2021) and a 1-month follow-up period for each patient. This is the first prospective study designed to describe the radiation dose according to the complexity of the procedure. Another strength of this study is that radiologic variables are obtained directly from the C-arm and no additional measurements are required for feasibility benefit. The results from this study will help us determine the level of radiation in different endovascular procedures, in view of their complexity.