bluuebunny/crossref_metadata_2025 huggingface dataset:
CGAOA-AttBiGRU: A Novel Deep Learning Framework for Forecasting CO2 Emissions
Authors: Liu Haijun , Wu Yang , Tan Dongqing , Chen Yi , Wang Haoran
Published: September 2024

Accurately predicting carbon dioxide (CO2) emissions is crucial for environmental protection. Currently, there are two main issues with predicting CO2 emissions: (1) existing CO2 emission prediction models mainly rely on Long Short-Term Memory (LSTM) and Gate Recurrent Unit (GRU) models, which can only model unidirectional temporal features, resulting in insufficient accuracy: (2) existing research on CO2 emissions mainly focuses on designing predictive models, without paying attention to model optimization, resulting in models being unable to achieve their optimal performance. To address these issues, this paper proposes a framework for predicting CO2 emissions, called CGAOA-AttBiGRU. In this framework, Attentional-Bidirectional Gate Recurrent Unit (AttBiGRU) is a prediction model that uses BiGRU units to extract bidirectional temporal features from the data, and adopts an attention mechanism to adaptively weight the bidirectional temporal features, thereby improving prediction accuracy. CGAOA is an improved Arithmetic Optimization Algorithm (AOA) used to optimize the five key hyperparameters of the AttBiGRU. We first validated the optimization performance of the improved CGAOA algorithm on 24 benchmark functions. Then, CGAOA was used to optimize AttBiGRU and compared with 12 optimization algorithms. The results indicate that the AttBiGRU optimized by CGAOA has the best predictive performance.
An Efficient Tour Construction Heuristic for Generating the Candidate Set of the Traveling Salesman Problem with Large Sizes
Authors: Tüű-Szabó Boldizsár , Földesi Péter , Kóczy László T.
Published: September 2024

In this paper, we address the challenge of creating candidate sets for large-scale Traveling Salesman Problem (TSP) instances, where choosing a subset of edges is crucial for efficiency. Traditional methods for improving tours, such as local searches and heuristics, depend greatly on the quality of these candidate sets but often struggle in large-scale situations due to insufficient edge coverage or high time complexity. We present a new heuristic based on fuzzy clustering, designed to produce high-quality candidate sets with nearly linear time complexity. Thoroughly tested on benchmark instances, including VLSI and Euclidean types with up to 316,000 nodes, our method consistently outperforms traditional and current leading techniques for large TSPs. Our heuristic’s tours encompass nearly all edges of optimal or best-known solutions, and its candidate sets are significantly smaller than those produced with the POPMUSIC heuristic. This results in faster execution of subsequent improvement methods, such as Helsgaun’s Lin–Kernighan heuristic and evolutionary algorithms. This substantial enhancement in computation time and solution quality establishes our method as a promising approach for effectively solving large-scale TSP instances.
Synthesis and Characterization of a Novel Chitosan-Based Nanoparticle–Hydrogel Composite System Promising for Skin Wound Drug Delivery
Authors: Huang Yueying , Hao Shuting , Chen Jiayu , Wang Mengyuan , Lin Ziheng , Liu Yanan
Published: September 2024

As a natural preservative, nisin is widely used in the food industry, while its application in biomedicine is limited due to its susceptibility to interference from external conditions. In this study, a nanoparticle–hydrogel composite system was designed to encapsulate and release nisin. Nisin nanoparticles were identified with a smooth, spherical visual morphology, particle size of 122.72 ± 4.88 nm, polydispersity coefficient of 0.473 ± 0.063, and zeta potential of 23.89 ± 0.37 mV. Based on the sample state and critical properties, three temperature-sensitive hydrogels based on chitosan were ultimately chosen with a rapid gelation time of 112 s, outstanding reticular structure, and optimal swelling ratio of 239.05 ± 7.15%. The composite system exhibited the same antibacterial properties as nisin, demonstrated by the composite system’s inhibition zone diameter of 17.06 ± 0.83 mm, compared to 20.20 ± 0.58 mm for nisin, which was attributed to the prolonged release effect of the hydrogel at the appropriate temperature. The composite system also demonstrated good biocompatibility and safety, making it suitable for application as short-term wound dressings in biomedicine due to its low hemolysis rate of less than 2%. In summary, our nanoparticle-based hydrogel composite system offers a novel application form of nisin while ensuring its stability, thereby deepening and broadening the employment of nisin.
Diagnostic Accuracy of Five Molecular Assays for the Detection of Dengue Virus
Authors: Scarpaleggia Marianna , Garzillo Giada , Lucente Miriana , Fraccalvieri Chiara , Randazzo Nadia , Massaro Elvira , Galano Barbara , Ricucci Valentina , Bruzzone Bianca , Domnich Alexander
Published: September 2024

Background and Objectives: The steady spread of dengue virus (DENV) poses a profound public health threat worldwide. Reverse transcription real-time polymerase chain reaction (RT2-PCR) has been increasingly recognized as a reference method for the diagnosis of acute dengue infection. The goal of this study was to assess the diagnostic accuracy of five different RT2-PCR kits for the detection of DENV in a historically processed set of sera samples. Materials and Methods: In this retrospective study, 25 sera samples from routinely processed unique adult patients with a known DENV status (previously tested in both molecular and serological assays) were tested in parallel using four conventional (RealStar Dengue PCR Kit 3.0, Clonit’ngo Zika, Dengue & Chikungunya, BioPerfectus Zika Virus/Dengue Virus/Chikungunya Virus Real Time PCR Kit and Novaplex Tropical fever virus) and one sample-to-result (STANDARD M10 Arbovirus Panel) RT2-PCR assays. Additionally, an end-point dilution analysis was conducted in quintuplicate on six serial dilutions of an RNA preparation obtained from a culture-grown DENV serotype 1 strain for a total of 150 tests. Results: The overall accuracy of the evaluated tests ranged from 84% to 100%. In particular, the sensitivity of three conventional RT2-PCR assays (RealStar, Clonit’ngo and Novaplex) was 100% (95% CI: 79.6–100%), while it was lower (73.3%; 95% CI: 48.1–89.1%) for the BioPerfectus kit. The sample-to-result STANDARD M10 panel performed comparatively well, showing a sensitivity of 92.9% (95% CI: 68.5–98.7%). No false positive results were registered in any assay. The end-point dilution analysis suggested that the RealStar kit had the lowest limit of detection. Conclusions: Available RT2-PCR kits for the detection of DENV are highly specific and generally sensitive and, therefore, their implementation in diagnostic pathways is advisable.
The Role of Monocyte Distribution Width in the Early Prediction of Sepsis in Patients Undergoing Cardiovascular Surgery: A Cross-Sectional Study
Authors: Özer Abdullah , Tak Sercan , Demirtaş Hüseyin , Yıldırım Alperen Kutay , Şimşek Elif , Oktar Gürsel Levent , Kaya Zühre
Published: September 2024

Background and Objectives: This is the first study to examine the role of monocyte distribution width (MDW) in predicting sepsis after cardiovascular surgery. Methods: This study included 43 consecutive patients who had undergone cardiovascular surgery between July 2021 and July 2022. All patients were examined at the following three time points (TPs): preoperative period (TP1), postoperative at 24 h (TP2), and discharge (TP3). SOFA score, leukocyte count, neutrophil-to-lymphocyte ratio (NLR), MDW, C-reactive protein (CRP), and procalcitonin (PCT) levels were tested at each TPs. The Sepsis-3 criteria were used to diagnose patients with sepsis. Results: The mean values of all variables (leukocyte count, NLR, MDW, CRP, and PCT levels) were significantly higher at TP2 and TP3 than at TP1 (p < 0.05). All these values were significantly higher at TP2 than at TP3 (p < 0.05). Patients with sepsis had significantly higher mean values for leukocyte count, NLR, MDW, CRP, and PCT levels than those without sepsis (p < 0.05). There was a significant correlation between MDW and inflammatory markers (CRP, PCT, and NLR) during the three time periods (p < 0.05). According to the ROC analysis, the optimal MDW cutoff value with the highest sensitivity and specificity for predicting sepsis in the postoperative period was 20.5. Conclusions: Our findings indicate that elevated MDW levels may be a valuable predictor of sepsis in patients following cardiovascular surgery.
Epidemiology and Prevalence of Musculoskeletal Disabilities Following Motor Vehicle Accidents in Aljouf Region, Saudi Arabia
Authors: Elsherbini Dalia Mahmoud Abdelmonem , Ali Lashin Saad , Allam Nesma M. , Elshorbagy Radwa T. , Eladl Hadaya Mosaad , Ibrahim Ateya Megahed , Elbastawisy Yasser M. , Eldesoqui Mamdouh , El-Sherbiny Mohamed
Published: September 2024

Background and Objectives: Motor vehicle accidents (MVAs) are the leading cause of disability, particularly among young adults in Saudi Arabia. Persistent disabilities account for around 7% of all injuries attributed to MVAs in Saudi Arabia in the last twenty years. Limited studies on musculoskeletal disabilities following MVAs have been carried out in Saudi Arabia. This study aims to explore the epidemiology and prevalence of musculoskeletal disabilities in motor vehicle accident (MVA) patients in the Aljouf region, Saudi Arabia. Materials and Methods: This retrospective cross-sectional study evaluated all MVA victims treated in the Aljouf region, Saudi Arabia, from January 2020 to December 2022. A total of 3252 medical records were collected, with 731, 1197, and 1324 musculoskeletal injury cases per year, of which 88, 168, and 153 records from 2020, 2021, and 2022 were analysed, respectively. Results: The study found that patients aged 25–34 and 35–44 years were the most likely to experience disability following MVAs. The difference between age groups during a single year was statistically significant (p < 0.001). Most patients were male (89.8%, 82.7%, and 79.7%) during 2020, 2021, and 2022, respectively. The majority of injuries involved the upper extremities (38.6%, 36.9%, and 40.5%), followed by lower extremities (36.4%, 35.7%, and 34.6%), head and neck (21.6%, 26.2%, and 34.6%), thoracic region (20.5%, 24.4%, and 17%), and finally lumbosacral spine (6.8%, 5.4%, and 6.5%) during 2020, 2021, and 2022, respectively, with a significant difference for each year (p < 0.001). The study found a link between the likelihood of developing high disability grades and injury severity scores. The patients with very severe ISS ≥ 25 (OR: ∞ CI 95%: 39.81–∞; p < 0.001), severe ISS = 16–24 (OR: ∞ CI 95%: 20.90–∞; p < 0.001), and moderate ISS = 9–15 (OR: ∞ CI 95%: 1.2–∞; p = 0.02) were at greater risk of developing high grades of disability. Conclusions: This study highlighted the musculoskeletal disabilities in the Aljouf region following MVAs. Severe musculoskeletal disabilities were rare, but fractures were the most common following MVAs. More efforts should be directed towards education on early transportation and transfer to the nearest medical centres, seeking assistance immediately after MVAs for patient safety, and disability prevention.
Gradual Reperfusion in Cardioplegia-Induced Cardiac Arrest
Authors: von Zeppelin Mascha , Hecker Florian , Keller Harald , Hlavicka Jan , Walther Thomas , Moritz Anton , Arsalan Mani , Holubec Tomas
Published: September 2024

Background and Objectives: The majority of cardiac surgical procedures are performed using cardiopulmonary bypass and cardioplegia-induced cardiac arrest. Cardiac arrest and reperfusion may lead to ischemia-reperfusion injury of the myocardium. The aim of this study was to investigate whether gradual reperfusion with a slow increase in oxygen partial pressure leads to a reduction in reperfusion injury. Materials and Methods: Fifty patients undergoing elective cardiac surgery were included in this prospective randomized study. Patients in the hyperoxemic (control) group received conventional reoxygenation (paO2 250–300 mmHg). Patients in the normoxemic (study) group received gradual reoxygenation (1st-minute venous blood with paO2 30–40 mmHg, 2nd-minute arterial blood with paO2 100–150 mmHg). Periprocedural blood samples were taken serially, and markers of myocardial injury were analyzed. In addition, the influence of gradual reoxygenation on hemodynamics, inflammation, and the overall perioperative course was evaluated. Results: There was a trend toward higher CK levels in the hyperoxemia group without statistical significance; however, CK-MB and troponin T levels did not show any statistical difference between the two groups. Potassium concentrations in the coronary sinus were significantly higher in the hyperoxemia group at 3 and 8 min after opening of the aortic cross-clamp (6.88 ± 0.87 mmol/L vs. 6.30 ± 0.91 mmol/L and 5.87 ± 0.73 mmol/L vs. 5.43 ± 0.42 mmol/L, respectively; p = 0.03 and p = 0.02). All other measurements did not show a statistical difference between the two groups. Conclusions: The use of gradual reperfusion in cardiac surgery with cardiopulmonary bypass and cardiac arrest is safe. However, it does not reduce ischemia-reperfusion injury compared to standard hyperoxemic reperfusion.
The Effect of Cardiac Rehabilitation in Paediatric Fontan Circulation Patients: A Prospective Intervention Study
Authors: Ven Luna van de , Félix Ana Clara , Suarez Joana , Rodrigues Bruno , Dias Jorge , Pinto Fátima F. , Laranjo Sérgio
Published: September 2024

Background and Objectives: The Fontan procedure, a palliative surgery for univentricular heart physiology, often reduces exercise capacity and quality of life. This study aimed to evaluate the impact of cardiac rehabilitation (CR) on improving outcomes in Fontan patients to inform evidence-based care. Materials and Methods: Fontan patients aged 8–30 participated in a structured CR program for at least three months. The program included weekly aerobic and resistance training sessions with educational and nutritional guidance. Baseline and post-CR assessments included cardiac function, fitness, daily activity, and health-related quality of life (HRQOL). Results: The cohort included ten Fontan patients, of whom six had a right systemic ventricle. CR significantly improved cardiorespiratory fitness, as seen in VO2 max (from 27.92 ± 5.15 to 34.69 ± 1.14 mL/kg/min, p = 0.0089) and percent predicted VO2 (from 0.67 ± 0.18 to 0.90 ± 0.02, p = 0.005). VCO2 increased by +8.68 ± 8.59 mL/kg/min but did not reach statistical significance (p = 0.05). Most haemodynamic and ventilatory parameters showed no significant improvement. All the SF-36 questionnaire domains showed significant HRQOL gains (p < 0.001). High adherence (85–93%), no adverse events, and reduced NT-proBNP levels supported the program’s safety. Conclusions: This study’s findings have important implications for the care of Fontan patients. CR significantly improved exercise capacity and HRQOL in Fontan patients across various anatomies, particularly in the right systemic ventricle. Integrating physical conditioning into standard care could reduce long-term morbidity and mortality; however, further research is needed to refine the protocols and confirm sustained benefits.
Water Separation and Formation of Cells with Differential Aeration as Factors Controlling Corrosion of Steel Pipelines in a Crude Oil Storage Facility
Authors: Šefl Václav , Shrestha Rojina , Prošek Tomáš
Published: September 2024

The mechanism causing the dramatic intensification of the corrosion deterioration of carbon steel pipes in a crude oil storage facility has been investigated. This study considers a number of factors affecting corrosion in crude oil, such as the water content, the corrosivity of the aqueous phase, the kinetics of water–oil separation, the effect of dissolved oxygen, the effect of the crude oil quality, the degree of stagnancy inside of the pipes, the possible contribution of microbially induced corrosion (MIC) and the presence of deposits. The key root of the corrosion intensification was the separation of the water phase, supported by stagnancy, which eventually led to the formation of stable shallow pits surrounded by cathodic areas.
Comprehensive Secondary Metabolite Profiling and Antioxidant Activity of Aqueous and Ethanol Extracts of Neolamarckia cadamba (Roxb.) Bosser Fruits
Authors: Yang Lin , Wu Liyan , Li Yongxin , Yang Yuhui , Gu Yuting , Yang Jialin , Zhang Luzy , Meng Fanxin
Published: September 2024

Background: Neolamarckia cadamba (Rubiaceae) is a well-recognized medicinal plant with recorded therapeutical attributes. However, a thorough assessment of active compounds in its fruits is lacking, limiting their use and valorization in pharmacological industries. Methods: Thus, this study investigated variations in the fruits’ secondary metabolite (SM) profiles, as well as antioxidant activities in aqueous (WA) and ethanol (ET) extracts. Results: Liquid chromatography–electrospray ionization tandem mass spectrometry identified 541 SMs, of which 14 and 1 (di-O-glucosylquinic acid) were specifically detected in ET and WA, respectively. Phenolic acids (36.97%), flavonoids (28.10%), terpenoids (12.20%), and alkaloids (9.98%) were the dominant SMs. The SM profiles of the fruits in WA and ET were quite different. We revealed 198 differentially extracted (DE) metabolites between WA and ET, including 62 flavonoids, 57 phenolic acids, 45 terpenoids, 14 alkaloids, etc. Most DE flavones (36 out of 40), terpenoids (45 out of 45), and alkaloids (12 out of 14) had higher content in ET. Catechin and its derivatives, procyanidins, and tannins had higher content in WA. ABTS and DPPH assays showed that the antioxidant activity of ET was significantly higher than that of WA. Conclusions: Our findings will facilitate the efficient extraction and evaluation of specific active compounds in N. cadamba.
Changes in Periodontal Parameters and Microbiome Composition of Periodontal Pocket in Patients with Chronic Inflammatory Diseases Receiving Targeted Anti-Cytokine Therapy
Authors: Wagner Juliane , Haker Luisa , Mewes Louisa , Bang Corinna , Rühlemann Malte , Naujokat Hendrik , Spille Johannes Heinrich , Lieb Wolfgang , Franke Andre , Schreiber Stefan , Laudes Matthias , Dörfer Christof , Wiltfang Jörg , Graetz Christian , Schulte Dominik Maria
Published: September 2024

Periodontitis is associated with systemic chronic inflammatory diseases. There is limited evidence on the influence of anti-cytokine therapies on the periodontal condition and microbiome in the tooth pocket of such patients, so the aim of this study was to elucidate this issue. In this observational trial, the periodontal status and the gingival crevicular fluid of 13 patients with different chronic inflammatory diseases were obtained before the initiation of anti-cytokine treatment and 14 weeks after. Gingival crevicular fluid was collected for 16S rRNA gene sequencing from a clinically healthy tooth and the deepest measured pocket. The Shannon Diversity Index significantly increased in the deepest pockets of patients (p = 0.039). The data showed alterations in the diversity of the subgingival microbiome over the course of the study, implying a shift towards a healthier condition after starting anti-cytokine therapy. Additional investigations are needed to analyze whether the administration of selective biologicals can improve periodontal conditions in patients with or without chronic inflammatory diseases.
Extended Reality Educational System with Virtual Teacher Interaction for Enhanced Learning
Authors: Liarokapis Fotis , Milata Vaclav , Skola Filip
Published: September 2024

Advancements in technology that can reshape educational paradigms, with Extended Reality (XR) have a pivotal role. This paper introduces an interactive XR intelligent assistant featuring a virtual teacher that interacts dynamically with PowerPoint presentations using OpenAI’s ChatGPT API. The system incorporates Azure Cognitive Services for multilingual speech-to-text and text-to-speech capabilities, custom lip-syncing solutions, eye gaze, head rotation and gestures. Additionally, panoramic images can be used as a sky box giving the illusion that the AI assistant is located at another location. Findings from three pilots indicate that the proposed technology has a lot of potential to be used as an additional tool for enhancing the learning process. However, special care must be taken into privacy and ethical issues.
Association between Visceral Adiposity Index and Hyperuricemia among Steelworkers: The Moderating Effects of Drinking Tea
Authors: Huang Xun , Zhong Zixin , He Junwei , Them Seydaduong , Chen Mengshi , Liu Aizhong , Tan Hongzhuan , Wen Shiwu , Deng Jing
Published: September 2024

Background/Objectives: Steelworkers are more likely to have a higher prevalence of hyperuricemia due to their exposure to special occupational factors and dietary habits. The interrelationships of visceral adiposity index (VAI), hyperuricemia, and drinking tea remain uncertain. This study aimed to assess the association between VAI and hyperuricemia among steelworkers, and if drinking tea modified this association. Methods: A total of 9928 steelworkers from Hunan Hualing Xiangtan Iron and Steel Company participated in this cross-sectional study. All participants completed a questionnaire, received anthropometric measurements, and provided blood samples for biochemical testing. Three logistic regression models were used to analyze the association between VAI and hyperuricemia. Results: In this study, the prevalence of hyperuricemia was approximately 23.74% (males: 24.41%; females: 20.63%), and a positive correlation between VAI and hyperuricemia risk was observed. In multivariate logistic regression analysis, the risk of hyperuricemia increased 1.76 times (95% CI: 1.64–1.89) and 2.13 times (95% CI: 1.76–2.57) with the increase of ln VAI in males and females, respectively. For males, compared to quartile 1, the risk of hyperuricemia in the second, third, and fourth quartile of VAI were 1.75 (95% CI: 1.11–2.71), 2.56 (95% CI: 1.67–3.93) and 4.89 (95% CI: 3.22–7.43). For females, compared to quartile 1, the risk of hyperuricemia in the second, third, and fourth quartile of VAI were 1.99 (95% CI: 1.40–2.82), 2.92 (95% CI: 1.96–4.34) and 4.51 (95% CI: 2.89–7.02). Additionally, our study found that, compared with not consuming tea, drinking tea could reduce uric acid levels by 0.014 in male steelworkers (t = −2.051, p = 0.040), 0.020 in workers consuming smoked food (t = −2.569, p = 0.010), and 0.022 in workers consuming pickled food (t = −2.764, p = 0.006). Conclusions: In conclusion, VAI is positively correlated with hyperuricemia in steelworkers. Drinking tea may lower uric acid levels in male steelworkers and steelworkers who prefer smoked and pickled foods.
Application of Bioelectrical Impedance Analysis in Weight Management of Children with Spina Bifida
Authors: Bagińska-Chyży Joanna , Korzeniecka-Kozerska Agata
Published: September 2024

Background: Children with spina bifida (SB) face an elevated risk of obesity, which necessitates precise methods for assessing body composition to ensure effective weight management. Conventional measures like BMI are inadequate for this population because of variations in growth patterns and skeletal structure. Bioelectrical impedance analysis (BIA) is a method that offers a clearer picture of body composition, yet its use in children with SB remains underexplored. Methods: Conducted on 57 children with SB and 28 healthy controls, with a median age of 11 years, this study evaluated anthropometrics, including BMI and BIA-derived metrics. The Hoffer’s scale to assess physical activity was applied in SB children. Results: Results showed that while 32% of SB patients were classified as overweight or obese based on BMI, 62% exhibited high body fat percentage via BIA. Fat-free mass, muscle and fat mass, and fat-to-muscle ratio (FMR) differed significantly compared to the reference group. Non-ambulators showed a higher median body fat mass percentage (25.9% vs. 17.8%, p = 0.01) and FMR (0.92 vs. 0.44, p = 0.003) in comparison to the community walkers. Conclusions: In SB children, BIA-measured fat mass is a better obesity indicator than BMI. Non-ambulatory, SB patients with obesity had the highest FMR values, indicating a higher risk for metabolic syndrome.
Determining the Levels of Cortisol, Testosterone, Lactic Acid and Anaerobic Performance in Athletes Using Various Forms of Coffee
Authors: Gür Melda , Çınar Vedat , Akbulut Taner , Bozbay Kenan , Yücedal Polat , Aslan Mehdi , Avcu Gökçe , Padulo Johnny , Russo Luca , Rog Joanna , Migliaccio Gian Mario
Published: September 2024

Background: Coffee is considered one of the most preferred and consumed beverage types in the world, and caffeine is known to increase physical performance due to its ergogenic properties. The aim of this study is to examine the effects of coffee consumption in different forms on cortisol, testosterone, lactic acid and anaerobic performance levels. Methods: A total of 15 licensed male football players participated in the research voluntarily. The research was implemented in a single-blind, counterbalanced, randomized and crossover study design. Participants were given caffeinated coffee (CK), decaffeinated coffee (placebo) (DK), powdered caffeine (in a gelatin capsule) (PC) and powdered placebo (maltodextrin in a capsule) (PM) on different days, and the Wingate test protocol was performed after the warm-up protocol. Blood samples were collected post-test. Cortisol, testosterone and lactic acid levels in the serum samples taken were determined by the ELISA method. Results: As a result, it was revealed that caffeinated coffee given to participants who exercise increased anaerobic power. However, it was observed that lactic acid levels were higher in placebo and decaffeinated coffee. The highest level of cortisol was found in caffeinated coffee and powdered caffeine compared to the placebo. Testosterone values were observed to be highest in caffeinated coffee and decaffeinated coffee compared to a placebo. Conclusions: The study suggests that the type of caffeine is a factor that affects absorption rate, which impacts performance and hormone levels.
Prolonged Copper Supplementation Modified Minerals in the Kidney, Liver and Blood, and Potentiated Oxidative Stress and Vasodilation of Isolated Aortic Rings in Young Wistar Rats
Authors: Kitala-Tańska Klaudia , Hanć Anetta , Juśkiewicz Jerzy , Majewski Michał
Published: September 2024

Background: Previous studies have highlighted that copper supplementation at 200% of the recommended daily dietary allowance modified vascular contraction and relaxation through increased reactive oxygen species (ROS) and prostaglandin formation, which modified the antioxidant status of middle-aged Wistar rats. Methods: In this study, young (1 month old) male Wistar rats (n/group = 10) received a diet supplemented with 6.45 mg copper/kg (100% of daily recommendation—Group A) for 8 weeks. The experimental group received 12.9 mg copper/kg of diet (200% of the daily recommendation—Group B). Results: Experimental supplementation with 200% copper modified the copper concentration in the blood (1.21-fold, p = 0.04), liver (1.15-fold, p = 0.032), and kidneys (1.23-fold, p = 0.045), potentiated the ROS formation in the aortic rings, and enhanced the sensitivity of the aortic rings to the vasodilator acetylcholine. We observed an increased participation of nitric oxide (NO) derived from inducible NO synthase (iNOS) in vascular contraction and a decreased net effect of vasodilator prostanoids derived from cyclooxygenase-2 in vascular relaxation. In rat kidneys, the concentrations of potassium (1.08-fold, p = 0.001) and iron (1.13-fold, p = 0.046) were higher, while, calcium (0.88-fold, p = 0.001) and chromium (0.77-fold, p = 0.005) concentrations were lower. In the rat liver, magnesium (1.06-fold, p = 0.012) was higher. No differences were observed in the concentrations of sodium, zinc, manganese, selenium, cobalt, molybdenum, and vanadium. The antioxidant activity of water- and lipid-soluble compounds; total antioxidant status in the blood; and superoxide dismutase, catalase, and malondialdehyde levels in the heart did not change. Conclusions: In young rats, prolonged supplementation with 200% copper had a lesser effect than anticipated on oxidative stress and vascular reactivity. Detailed data on the status of trace elements and their interactions in patients of different age groups are strongly required for effective nutritional and therapeutic intervention.
Dietary Determinants of Metabolic and Gut Microbial Health in Patients with Inflammatory Bowel Disease
Authors: Wark Gabrielle , Kaakoush Nadeem O. , Samocha-Bonet Dorit , Ghaly Simon , Danta Mark
Published: September 2024

Background: Diet has been linked to gut dysbiosis and the onset, course, and response to treatment of patients with IBD and metabolic disease. Methods: This single-centre prospective case-control study investigated the relationship between dietary intake, metabolic profile, and stool microbial composition in 57 individuals with IBD in clinical remission and 24 healthy individuals (HC). Participants’ baseline anthropometric measurements, serum metabolic parameters, lipid profiles, and oral and stool samples for microbiota testing were collected. Their dietary intake and physical activity were documented. A partially corrected correlation was performed to examine the associations between variables and p-values adjusted for multiple comparisons using the Benjamini–Hochberg equation (adj-p). Results: In participants with IBD, the intake of saturated fat correlated positively, and the intake of dietary fibre correlated negatively with anthropometric indices (saturated fat and BMI: r = 0.37, adj-p = 0.04, fibre and BMI: r = −0.45, adj-p = 0.01). Higher anthropometric indices were associated with poorer glucose control and a less favourable serum lipid profile (BMI and insulin: r = 0.48, p < 0.01, WHR and triglycerides: r = 0.57, p < 0.01). The stool microbiota of participants in the IBD group was less diverse and more similar to their oral microbiota than was observed in the HC group (Mann–Whitney U test p = 0.03). Within the IBD group, a higher intake of added sugar and processed meat and a higher serum insulin level was associated with lower stool microbial alpha diversity (processed meat intake and Shannon’s diversity: r = −0.43, adj-p = 0.02; added sugar and Shannon’s diversity: r = −0.39, adj-p = 0.03; insulin and Shannon’s diversity: r = −0.45, adj-p = 0.02). Neither the dietary intake nor stool microbial composition correlated with the risk of disease flaring. Conclusions: Our findings suggest that dietary intake is associated with the metabolic health and gut microbial composition of IBD patients.
An Overview of Aspergillus Species Associated with Plant Diseases
Authors: Zakaria Latiffah
Published: September 2024

The genus Aspergillus contains several species that are important plant pathogens. Plant pathogenic Aspergillus spp. affect agricultural crops in the field as well as after harvest, often associated with corn ear rot, cotton boll rot, peanut yellow mold, black mold of onion and garlic, fruit rot on grapes, pomegranates, olives, citrus, and apples. Coffee berries and coffee beans as well as tree nuts are also frequently infected by Aspergillus spp. Some of the plant pathogenic Aspergillus spp. are also mycotoxigenic, produced mycotoxin in the plant tissues leading to contamination of agricultural products. Over the years, reports of plant diseases caused by Aspergillus in various crops have increased, suggesting they are commonly encountered plant pathogens. This review focuses on agricultural crops or cultivated plants infected by Aspergillus spp. The compilation of plant pathogenic Aspergillus spp. provides information to mycologists, particularly those involved in plant pathology and crop protection, with updated information on plant diseases caused by various species of Aspergillus. The updated information also includes the locality or location, province, state and the country. The knowledge on the prevalence and geographic distribution of plant pathogenic Aspergillus spp. is beneficial in the application of crop protection.
Patient Experiences and Perceptions with Infections Due to Multidrug-Resistant Organisms: A Systematic Review
Authors: AlRawashdeh Mousa M. , Ishak Angela , Al-Bunnia Ahmed , Agouridis Aris P. , Lytras Theodore , Spernovasilis Nikolaos , Tsioutis Constantinos
Published: September 2024

Infections by multidrug-resistant organisms (MDROs) pose significant public health challenges, including increased mortality rates, healthcare costs, and significant impacts on the quality of life for patients. Utilizing a systematic review methodology adhering to PRISMA guidelines, we performed a comprehensive search across three databases, identifying 20 relevant studies that investigated the psychological effects of infections due to MDROs on hospitalized adults. The primary outcomes examined included depression, anxiety, and other psychosocial impacts, while secondary outcomes included patient and caregiver understanding of the infection. Findings revealed consistent associations between contact isolation due to MDRO infections and heightened levels of depression and anxiety among patients, although evidence regarding the impact on anger was mixed. Other psychological aspects, such as feelings of stigmatization and reduced healthcare provider interactions, were also recorded. The current systematic review highlights the importance of addressing these psychological effects through holistic, patient-centered care approaches, emphasizing the need for better communication and comprehensive education for both patients and healthcare providers. Our findings suggest that mitigating the psychological burden of MDROs can enhance overall patient care and outcomes and call for further research to optimize care strategies for patients hospitalized for infections due to MDROs.
Performance of Fujifilm Dengue NS1 Antigen Rapid Diagnosis Kit Compared to Quantitative Real-Time Polymerase Chain Reaction
Authors: Ngwe Tun Mya Myat , Kapandji Merveille , Wada Atsuhiko , Yamamoto Ko , Dumre Shyam Prakash , Nwe Khine Mya , Lin Htin , Takamatsu Yuki , Thant Kyaw Zin , Thu Hlaing Myat , Urano Takeshi , Pandey Basu Dev , Morita Kouichi
Published: September 2024

Dengue is a viral infection caused by the dengue virus (DENV), transmitted to humans through the bite of infected Aedes mosquitoes. About half of the world’s population is now at risk of dengue, which represents a global public health concern, especially in tropical and subtropical countries. Early detection of the viral infection is crucial to manage the disease; hence, effective rapid diagnostic tests are essential. In this study, we evaluated the performance between the new Fujifilm Dengue non-structural antigen diagnosis kit (FF NS1 kit) and the SD Bioline NS1 antigen test kit (SD NS1 kit) against the quantitative real-time polymerase chain reaction (qRT-PCR) assays. The 140 acute serum samples collected from the Yangon General Hospital and Yangon Children’s Hospital, Myanmar, from 2017 to 2019 were characterised by the three assays. With the qRT-PCR as the standard, the FF NS1 kit and the SD NS1 kit exhibited sensitivity of 94.3% and 88.6%, respectively, and specificity of 100% in both kits. Moreover, the positivity rates of the FF NS1 kit and the SD NS1 kit were 97.5% and 95% in primary infection and 90% and 80% in secondary infection, respectively. Our overall results suggest that the FF NS1 kit is reliable and accurate for detecting DENV infection.
The Ubiquitous Wilt-Inducing Pathogen Fusarium oxysporum—A Review of Genes Studied with Mutant Analysis
Authors: Jackson Edan , Li Josh , Weerasinghe Thilini , Li Xin
Published: September 2024

Fusarium oxysporum is one of the most economically important plant fungal pathogens, causing devastating Fusarium wilt diseases on a diverse range of hosts, including many key crop plants. Consequently, F. oxysporum has been the subject of extensive research to help develop and improve crop protection strategies. The sequencing of the F. oxysporum genome 14 years ago has greatly accelerated the discovery and characterization of key genes contributing to F. oxysporum biology and virulence. In this review, we summarize important findings on the molecular mechanisms of F. oxysporum growth, reproduction, and virulence. In particular, we focus on genes studied through mutant analysis, covering genes involved in diverse processes such as metabolism, stress tolerance, sporulation, and pathogenicity, as well as the signaling pathways that regulate them. In doing so, we hope to present a comprehensive review of the molecular understanding of F. oxysporum that will aid the future study of this and related species.
Advances in the Search for SARS-CoV-2 Mpro and PLpro Inhibitors
Authors: Diogo Marcel Arruda , Cabral Augusto Gomes Teixeira , de Oliveira Renata Barbosa
Published: September 2024

SARS-CoV-2 is a spherical, positive-sense, single-stranded RNA virus with a large genome, responsible for encoding both structural proteins, vital for the viral particle’s architecture, and non-structural proteins, critical for the virus’s replication cycle. Among the non-structural proteins, two cysteine proteases emerge as promising molecular targets for the design of new antiviral compounds. The main protease (Mpro) is a homodimeric enzyme that plays a pivotal role in the formation of the viral replication–transcription complex, associated with the papain-like protease (PLpro), a cysteine protease that modulates host immune signaling by reversing post-translational modifications of ubiquitin and interferon-stimulated gene 15 (ISG15) in host cells. Due to the importance of these molecular targets for the design and development of novel anti-SARS-CoV-2 drugs, the purpose of this review is to address aspects related to the structure, mechanism of action and strategies for the design of inhibitors capable of targeting the Mpro and PLpro. Examples of covalent and non-covalent inhibitors that are currently being evaluated in preclinical and clinical studies or already approved for therapy will be also discussed to show the advances in medicinal chemistry in the search for new molecules to treat COVID-19.
“I Solemnly Swear”: A Comparative Study of Codes of Professional Ethics amongst Pharmacists from Culturally Diverse European Countries
Authors: Raimundo Raquel , Cavaco Afonso
Published: September 2024

Ethical practice is a universal concern for healthcare professionals, independent of their social, cultural, or religious background. This study aimed to assess and categorise statements published in codes of ethics for pharmacists from three diverse societies within the wider European area. The study followed a qualitative exploratory and triangular design, comparing the leading professional and ethical statements between three geographically apart countries (Portugal, Lithuania, and Turkey) and using the International Pharmaceutical Federation Code of Ethics proposal as a gold standard. Common core values such as honesty, integrity, and professional autonomy were identified across the countries’ codes, suggesting that shared recognised core values underpin pharmacists’ practice and policies in culturally diverse settings. None of the codes fully correspond to the framework the International Pharmaceutical Federation proposed. The analysis elicited significant inconsistencies between the codes for analogous practice models within the same continent. Further studies are needed to gain a more profound and comprehensive understanding of the underlying reasons for these discrepancies so that ethical weaknesses can be improved and harmonisation towards best-practice principles can benefit patients and healthcare systems.
Agro-Physiological and DNA Methylation Responses to Salinity Stress in Wheat (Triticum aestivum L.), Aegilops cylindrica Host, and Their Introgressed Lines
Authors: Hoseini Mohsen , Arzani Ahmad , Saeidi Ghodratollah , Araniti Fabrizio
Published: September 2024

Bottlenecks, including limited genetic variation and the ongoing loss of genetic diversity, have hindered the development of modern wheat cultivars., making it crucial to use genetic diversity from wild relatives to improve wheat’s adaptation to abiotic stress, such as salinity. This study assessed the phenotypic and epigenetic variation of introgressed wheat lines (BC4F2) derived from hybridizing two wheat cultivars with Aegilops cylindrica (AC). This study assessed the phenotypic and epigenetic variation of 156 introgressed wheat lines (BC4F2) derived from hybridization between wheat cultivars “Chinese Spring” (CS) and “Roshan” (R) and Aegilops cylindrica (AC). These lines and their recurrent parents (total of 158) were evaluated under normal and saline field conditions for the agronomic traits and stress tolerance indices. The data were used to select the most tolerant and most sensitive lines. Then, the selected BC4F2 lines and their parents (AC, CS, and R) were subjected to physiological, DNA cytosine methylation, and expression analysis of HKT1;5, NHX1, and SOS1 genes under control and salt stress conditions. Agro-physiological, epigenetic, and gene expression analyses showed the significant effects of salt stress and genetic background, as well as the differential response of the BC4F2 lines to salt stress. The variations in leaf and root K, Na, and K/Na ratios, and leaf Chla, Chlb, Car, and MDA levels, unlike DPPH radical scavenging levels, between salt-tolerant and salt-sensitive BC4F2 lines under saline conditions indicated a substantial distinction in salinity tolerance responses. RT-qPCR indicated higher expression levels of NHX1 and SOS1 genes in the leaf and root tissues of tolerant lines than those of sensitive lines. Global leaf and root DNA methylation analysis revealed the significant effects of salinity on the methylation modifications and confirmed the successful introgression of the salt-tolerance epigenome from Ae. cylindrica into wheat. Exploiting the genetic diversity of wild wheat relatives is a crucial goal for increasing genetic and epigenetic variation to enhance plant adaptation to salt stress.
Effects of Sulfur Application on the Quality of Fresh Waxy Maize
Authors: Jiang Chenyang , Liang Yuwen , Wang Yuru , You Genji , Guo Jian , Lu Dalei , Li Guanghao
Published: September 2024

Balanced fertilizer application is crucial for achieving high-yield, high-quality, and efficient maize cultivation. Sulfur (S), considered a secondary nutrient, ranks as the fourth most essential plant nutrient after nitrogen (N), phosphorus (P), and potassium (K). S deficiency could significantly influence maize growth and development. Field experiments were conducted in Jiangsu, Yangzhou, China, from April 1 to July 20 in 2023. Jingkenuo2000 (JKN2000) and Suyunuo5 (SYN5) were used as experiment materials, and four treatments were set: no fertilizer application (F0), S fertilizer application (F1), conventional fertilization method (F2), and conventional fertilization method with additional S application (F3). The objective was to investigate the impact of S application on grain weight and the quality of fresh waxy maize flour and starch. The results indicated that all fertilization treatments significantly increased grain weight and the starch and protein contents in grains compared to no fertilization. Among these, F3 exhibited the most significant increases. Specifically, in JKN2000, the grain weight, starch content (SC), and protein content (PC) increased by 27.7%, 4.8%, and 14.8%, respectively, while in SYN5, these parameters increased by 26.3%, 6.2%, and 7.4%, respectively, followed by F2 and F1. Compared to F0, F3 increased starch and protein contents by 4.8% and 14.8% in JKN2000, and by 6.2% and 7.4% in SYN5. Compared to F0, F2 and F3 significantly increased the iodine binding capacity (IBC) of SYN5, with F3 being more effective than F2, while they had no significant effect on the IBC of JKN2000. The peak viscosity (PV) and breakdown viscosity (BD) of waxy maize flour and starch for both varieties showed a consistent response (increasing trend) to S application, and F3 had the largest increase. Regarding the thermal properties of waxy maize flour, F3 significantly enhanced the retrogradation enthalpy (ΔHgel) of both varieties compared to F0, while achieving the lowest retrogradation percentage (%R). In starch, the highest ΔHgel and the lowest %R were observed under the F2 treatment. In summary, under the conditions of this experiment, adding S fertilizer to conventional fertilization not only increased the grain weight of waxy maize but also effectively optimized the pasting and thermal properties of waxy maize flour and starch.
Decoding the Transcriptomics of Oil Palm Seed Germination
Authors: Suksa-Ard Padungsak , Nuanlaong Sunya , Pooljun Chettupon , Azzeme Azzreena Mohamad , Suraninpong Potjamarn
Published: September 2024

Seed dormancy and germination are critical factors affecting oil palm production efficiency. The typical dormancy-breaking process involves dry heat treatment (38–40 °C for 40–60 days) followed by germination at 30–32 °C. To understand the molecular mechanisms behind this process and improve germination rates and speed, we conducted transcriptome analysis at three stages: pre-incubation, 45-day incubation at 40 °C, and 14-day germination at 32 °C. Our findings, supported by qRT–PCR and DEGs analysis, identified four key stages: ABA degradation, energy mobilization, starch mobilization, and cell elongation and division. ABA pathway genes (SnRK2, PYR/PYL) were active during dormancy release, while GAE and GPI were upregulated after heat treatment, indicating increased energy metabolism and structural changes. During germination, genes involved in starch/sucrose metabolism (SPS, TPP, SS, MGAM) and cell wall biosynthesis (GAUT1, PE, GAE) supported embryo expansion, with BAM, PGM, GlgB fueling early growth. Auxin (TIR1, AUX/IAA, ARF), brassinosteroid (BRI1, BSK, BIN2, CYCD3), ethylene (ETR, CTR1), and jasmonic acid (JAR1, COI1) pathway genes regulated cell growth and stress response, promoting seedling development. Though gibberellins were not crucial for this oil palm variety, gene expression varied between varieties. This study provides information on oil palm seed germination that could be applied to other oil palm species, particularly in terms of incubation times and chemical treatments.