bluuebunny/crossref_metadata_2025 huggingface dataset:
Large-scale high-throughput 3D culture, imaging, and analysis of cell spheroids using microchip-enhanced light-sheet microscopy
Authors: Zhu Tingting , Nie Jun , Yu Tingting , Zhu Dan , Huang Yanyi , Chen Zaozao , Gu Zhongze , Tang Jiang , Li Dongyu , Fei Peng
Published: March 2023

Light sheet microscopy combined with a microchip is an emerging tool in biomedical research that notably improves efficiency. However, microchip-enhanced light-sheet microscopy is limited by noticeable aberrations induced by the complex refractive indices in the chip. Herein, we report a droplet microchip that is specifically engineered to be capable of large-scale culture of 3D spheroids (over 600 samples per chip) and has a polymer index matched to water (difference <1%). When combined with a lab-built open-top light-sheet microscope, this microchip-enhanced microscopy technique allows 3D time-lapse imaging of the cultivated spheroids with ∼2.5-µm single-cell resolution and a high throughput of ∼120 spheroids per minute. This technique was validated by a comparative study on the proliferation and apoptosis rates of hundreds of spheroids with or without treatment with the apoptosis-inducing drug Staurosporine.
Optrode recording of an entorhinal–cortical circuit in freely moving mice
Authors: Yang Xinyu , Chen Qianwei , Jian Tingliang , Du Haoran , Jin Wenjun , Liang Mengru , Wang Rui , Chen Xiaowei , Liao Xiang , Qin Han
Published: March 2023

The deep layers of medial entorhinal cortex (MEC) are considered a crucial station for spatial cognition and memory. The deep sublayer Va of MEC (MECVa) serves as the output stage of the entorhinal-hippocampal system and sends extensive projections to brain cortical areas. However, the functional heterogeneity of these efferent neurons in MECVa is poorly understood, due to the difficulty of performing single-neuron activity recording from the narrow band of cell population while the animals are behaving. In the current study, we combined multi-electrode electrophysiological recording and optical stimulation to record cortical-projecting MECVa neurons at single-neuron resolution in freely moving mice. First, injection of a viral Cre-LoxP system was used to express channelrhodopsin-2 specifically in MECVa neurons that project to the medial part of the secondary visual cortex (V2M-projecting MECVa neurons). Then, a lightweight, self-made optrode was implanted into MECVa to identify the V2M-projecting MECVa neurons and to enable single-neuron activity recordings in mice performing the open field test and 8-arm radial maze. Our results demonstrate that optrode approach is an accessible and reliable method for single-neuron recording of V2M-projecting MECVa neurons in freely moving mice, paving the way for future circuit studies designed to characterize the activity of MECVa neurons during specific tasks.
Deciphering Pancharatnam’s discovery of geometric phase: retrospective
Authors: Garza-Soto Luis , Hagen Nathan , Lopez-Mago Dorilian
Published: March 2023

While Pancharatnam discovered the geometric phase in 1956, his work was not widely recognized until its endorsement by Berry in 1987, after which it received wide appreciation. However, because Pancharatnam’s paper is unusually difficult to follow, his work has often been misinterpreted as referring to an evolution of states of polarization, just as Berry’s work focused on a cycle of states, even though this consideration does not appear in Pancharatnam’s work. We walk the reader through Pancharatnam’s original derivation and show how Pancharatnam’s approach connects to recent work in geometric phase. It is our hope to make this widely cited classic paper more accessible and better understood.
TDI-like multi-slit hyperspectral imaging for enhanced throughput via the Kalman filter
Authors: Hu Yaoyao , Liu Chengwen , Lim Zi Heng , Qi Yi , Sun Haoyang , Chang Jun , Zhou Guangya
Published: March 2023

The time-delay integration (TDI) technique is increasingly used to improve the signal-to-noise ratio (SNR) of remote sensing and imaging by exposing the scene multiple times. Inspired by the principle of TDI, we propose a TDI-like pushbroom multi-slit hyperspectral imaging (MSHSI) approach. In our system, multiple slits are used to significantly improve the throughput of the system, thereby enhancing the sensitivity and SNR through multiple exposures of the same scene during pushbroom scan. Meanwhile, a linear dynamic model for the pushbroom MSHSI is established, where the Kalman filter (KF) is employed to reconstruct the time-varying overlapped spectral images on a single conventional image sensor. Further, we designed and fabricated a customized optical system that can operate in both multi-slit and single slit modes to experimentally verify the feasibility of the proposed method. Experimental results indicate that the developed system improved SNR by a factor of about 7 compared to that of the single slit mode, while demonstrating excellent resolution in both spatial and spectral dimensions.
Superhydrophobic and easy-to-clean full-packing nanopatterned microlens array with high-quality imaging
Authors: Fang Chaolong , Xu Wangdong , Zhu Libo , Zhuang Youyi , Zhang Dawei
Published: March 2023

The high-quality imaging and easy cleaning property of microlens array (MLA) are two very important factors for its outdoor work. Herein, a superhydrophobic and easy-to-clean full-packing nanopatterned MLA with high-quality imaging is prepared by thermal reflow together with sputter deposition. Scanning electronic microscopy (SEM) images demonstrate that the sputter deposition method can improve 84% packing density of MLA prepared by thermal reflow to 100% and add nanopattern on the surface of microlens. The prepared full-packing nanopatterned MLA (npMLA) possess clear imaging with a significant increase of signal-to-noise ratio and higher transparency compared with the MLA prepared by thermal reflow. Besides for excellent optical properties, the full-packing surface displays a superhydrophobic property with a contact angle of 151.3°. Further, the full-packing contaminated by chalk dust become easier to be cleaned by nitrogen blowing and deionized water. As a result, the prepared full-packing is considered to be potential for various applications in the outdoor.
Single-shot refractive index slice imaging using spectrally multiplexed optical transfer function reshaping
Authors: Lee Chungha , Hugonnet Herve , Park Juyeon , Lee Mahn Jae , Park Weisun , Park YongKeun
Published: March 2023

The refractive index (RI) of cells and tissues is crucial in pathophysiology as a noninvasive and quantitative imaging contrast. Although its measurements have been demonstrated using three-dimensional quantitative phase imaging methods, these methods often require bulky interferometric setups or multiple measurements, which limits the measurement sensitivity and speed. Here, we present a single-shot RI imaging method that visualizes the RI of the in-focus region of a sample. By exploiting spectral multiplexing and optical transfer function engineering, three color-coded intensity images of a sample with three optimized illuminations were simultaneously obtained in a single-shot measurement. The measured intensity images were then deconvoluted to obtain the RI image of the in-focus slice of the sample. As a proof of concept, a setup was built using Fresnel lenses and a liquid-crystal display. For validation purposes, we measured microspheres of known RI and cross-validated the results with simulated results. Various static and highly dynamic biological cells were imaged to demonstrate that the proposed method can conduct single-shot RI slice imaging of biological samples with subcellular resolution.
Metasurfaces on silicon photonic waveguides for simultaneous emission phase and amplitude control
Authors: Hsieh Ping-Yen , Fang Shun-Lin , Lin Yu-Siang , Huang Wen-Hsieh , Shieh Jia-Min , Yu Peichen , Chang You-Chia
Published: March 2023

Chip-scale photonic systems that manipulate free-space emission have recently attracted attention for applications such as free-space optical communications and solid-state LiDAR. Silicon photonics, as a leading platform for chip-scale integration, needs to offer more versatile control of free-space emission. Here we integrate metasurfaces on silicon photonic waveguides to generate free-space emission with controlled phase and amplitude profiles. We demonstrate experimentally structured beams, including a focused Gaussian beam and a Hermite-Gaussian TEM10 beam, as well as holographic image projections. Our approach is monolithic and CMOS-compatible. The simultaneous phase and amplitude control enable more faithful generation of structured beams and speckle-reduced projection of holographic images.
Nonlocal generalized quantum measurement of product observables with mixed entanglement
Authors: Jiang Lan , Li Zhenhua , Li Tao
Published: March 2023

Nonlocal observables of spacelike separated quantum systems in combination with their measurements contribute greatly to quantum theory and its applications. We present a nonlocal generalized quantum measurement protocol for measuring product observables, assisted by a meter in a mixed entangled state rather than maximally or partially entangled pure states. By tuning the entanglement of the meter, measurement strength of arbitrary values can be achieved for nonlocal product observables, since measurement strength equals the concurrence of the meter. Furthermore, we present a specific scheme to measure the polarization of two nonlocal photons using linear optics. We refer to the polarization and spatial-mode degrees of freedom of the same photon pair as the system and the meter, respectively, which significantly simplifies the interaction between the system and the meter. This protocol can be useful for applications involving nonlocal product observables and nonlocal weak values, and for tests of quantum foundations in nonlocal scenarios.
An iterative BP-CNN decoder for optical fiber communication systems
Authors: Zhang Jing , Jiang Wenshan , Zhou Jiahao , Zhao Xue , Huang Xiatao , Yu Zhenming , Yi Xingwen , Qiu Kun
Published: March 2023

The conventional belief propagation (BP) of the low-density parity-check (LDPC) is designed based on additive white Gaussian noise (AWGN) close to the Shannon limit; however, the correlated noise due to chromatic dispersion or square-law detection results in a performance penalty in the intensity modulation and direct-detection (IM/DD) system. We propose an iterative BP cascaded convolution neural network (CNN) decoder to mitigate the correlated channel noise. We use a model of correlated Gaussian noise to verify that the noise correlation can be identified by the CNN and the decoding performance is improved by the iterative processing. We successfully demonstrate the proposed method in a 50-Gb/s 4-ary pulse amplitude modulation (PAM-4) IM/DD system. The simulation results show that the proposed decoder can achieve a BER performance improvement which is robust to transmission distance and launch optical power. The experimental results show that the iterative BP-CNN decoder outperforms the standard BP decoder by 1.2 dB in received optical power over 25-km SSMF.
A real data-driven simulation strategy to select an imputation method for mixed-type trait data
Authors: May Jacqueline A. , Feng Zeny , Adamowicz Sarah J.
Published: March 2023

Missing observations in trait datasets pose an obstacle for analyses in myriad biological disciplines. Considering the mixed results of imputation, the wide variety of available methods, and the varied structure of real trait datasets, a framework for selecting a suitable imputation method is advantageous. We invoked a real data-driven simulation strategy to select an imputation method for a given mixed-type (categorical, count, continuous) target dataset. Candidate methods included mean/mode imputation, k-nearest neighbour, random forests, and multivariate imputation by chained equations (MICE). Using a trait dataset of squamates (lizards and amphisbaenians; order: Squamata) as a target dataset, a complete-case dataset consisting of species with nearly complete information was formed for the imputation method selection. Missing data were induced by removing values from this dataset under different missingness mechanisms: missing completely at random (MCAR), missing at random (MAR), and missing not at random (MNAR). For each method, combinations with and without phylogenetic information from single gene (nuclear and mitochondrial) or multigene trees were used to impute the missing values for five numerical and two categorical traits. The performances of the methods were evaluated under each missing mechanism by determining the mean squared error and proportion falsely classified rates for numerical and categorical traits, respectively. A random forest method supplemented with a nuclear-derived phylogeny resulted in the lowest error rates for the majority of traits, and this method was used to impute missing values in the original dataset. Data with imputed values better reflected the characteristics and distributions of the original data compared to complete-case data. However, caution should be taken when imputing trait data as phylogeny did not always improve performance for every trait and in every scenario. Ultimately, these results support the use of a real data-driven simulation strategy for selecting a suitable imputation method for a given mixed-type trait dataset.
Fentanyl-driven acceleration of racial, gender and geographical disparities in drug overdose deaths in the United States
Authors: D’Orsogna Maria R. , Böttcher Lucas , Chou Tom
Published: March 2023

We examine trends in drug overdose deaths by race, gender, and geography in the United States during the period 2013–2020. Race and gender specific crude rates were extracted from the final National Vital Statistics System multiple cause-of-death mortality files for several jurisdictions and used to calculate the male-to-female ratios of crude rates between 2013 and 2020. We established 2013–2019 temporal trends for four major drug types: psychostimulants with addiction potential (T43.6, such as methamphetamines); heroin (T40.1); natural and semi-synthetic opioids (T40.2, such as those contained in prescription pain-killers); synthetic opioids (T40.4, such as fentanyl and its derivatives) through a quadratic regression and determined whether changes in the pandemic year 2020 were statistically significant. We also identified which race, gender and states were most impacted by drug overdose deaths. Nationwide, the year 2020 saw statistically significant increases in overdose deaths from all drug categories except heroin, surpassing predictions based on 2013–2019 trends. Crude rates for Black individuals of both genders surpassed those for White individuals for fentanyl and psychostimulants in 2018, creating a gap that widened through 2020. In some regions, mortality among White persons decreased while overdose deaths for Black persons kept rising. The largest 2020 mortality statistic is for Black males in the District of Columbia, with a record 134 overdose deaths per 100,000 due to fentanyl, 9.4 times more than the fatality rate among White males. Male overdose crude rates in 2020 remain larger than those of females for all drug categories except in Idaho, Utah and Arkansas where crude rates of overdose deaths by natural and semisynthetic opioids for females exceeded those of males. Drug prevention, mitigation and no-harm strategies should include racial, geographical and gender-specific efforts, to better identify and serve at-risk groups.
The social media diet: A scoping review to investigate the association between social media, body image and eating disorders amongst young people
Authors: Dane Alexandra , Bhatia Komal
Published: March 2023

Background

Eating disorders are a group of heterogenous, disabling and deadly psychiatric illnesses with a plethora of associated health consequences. Exploratory research suggests that social media usage may be triggering body image concerns and heightening eating disorder pathology amongst young people, but the topic is under-researched as a global public health issue.

Aim

To systematically map out and critically review the existing global literature on the relationship between social media usage, body image and eating disorders in young people aged 10–24 years.

Methods

A systematic search of MEDLINE, PyscINFO and Web of Science for research on social media use and body image concerns / disordered eating outcomes published between January 2016 and July 2021. Results on exposures (social media usage), outcomes (body image, eating disorders, disordered eating), mediators and moderators were synthesised using an integrated theoretical framework of the influence of internet use on body image concerns and eating pathology.

Results

Evidence from 50 studies in 17 countries indicates that social media usage leads to body image concerns, eating disorders/disordered eating and poor mental health via the mediating pathways of social comparison, thin / fit ideal internalisation, and self-objectification. Specific exposures (social media trends, pro-eating disorder content, appearance focused platforms and investment in photos) and moderators (high BMI, female gender, and pre-existing body image concerns) strengthen the relationship, while other moderators (high social media literacy and body appreciation) are protective, hinting at a ‘self-perpetuating cycle of risk’.

Conclusion

Social media usage is a plausible risk factor for the development of eating disorders. Research from Asia suggests that the association is not unique to traditionally western cultures. Based on scale of social media usage amongst young people, this issue is worthy of attention as an emerging global public health issue.
What, how and who: Cost-effectiveness analyses of COVID-19 vaccination to inform key policies in Nigeria
Authors: Ruiz Francis J. , Torres-Rueda Sergio , Pearson Carl A. B. , Bergren Eleanor , Okeke Chinyere , Procter Simon R. , Madriz-Montero Andres , Jit Mark , Vassall Anna , Uzochukwu Benjamin S. C.
Published: March 2023

While safe and efficacious COVID-19 vaccines have achieved high coverage in high-income settings, roll-out remains slow in sub-Saharan Africa. By April 2022, Nigeria, a country of over 200 million people, had only distributed 34 million doses. To ensure the optimal use of health resources, cost-effectiveness analyses can inform key policy questions in the health technology assessment process. We carried out several cost-effectiveness analyses exploring different COVID-19 vaccination scenarios in Nigeria. In consultation with Nigerian stakeholders, we addressed three key questions: what vaccines to buy, how to deliver them and what age groups to target. We combined an epidemiological model of virus transmission parameterised with Nigeria specific data with a costing model that incorporated local resource use assumptions and prices, both for vaccine delivery as well as costs associated with care and treatment of COVID-19. Scenarios of vaccination were compared with no vaccination. Incremental cost-effectiveness ratios were estimated in terms of costs per disability-adjusted life years averted and compared to commonly used cost-effectiveness ratios. Viral vector vaccines are cost-effective (or cost saving), particularly when targeting older adults. Despite higher efficacy, vaccines employing mRNA technologies are less cost-effective due to high current dose prices. The method of delivery of vaccines makes little difference to the cost-effectiveness of the vaccine. COVID-19 vaccines can be highly effective and cost-effective (as well as cost-saving), although an important determinant of the latter is the price per dose and the age groups prioritised for vaccination. From a health system perspective, viral vector vaccines may represent most cost-effective choices for Nigeria, although this may change with price negotiation.
Community engagement in health services research on soil-transmitted helminthiasis in Asia Pacific region: Systematic review
Authors: Naing Cho , Tung Wong Siew , Htet Norah Htet , Aung Htar Htar , Whittaker Maxine A.
Published: March 2023

The research question was what studies are available that have assessed community engagement in the health services research on soil-transmitted helminths? We aimed to synthesise evidence on how communities were engaged in health services research on soil-transmitted helminths in low-and-middle-income countries of the Asia-Pacific Region. We focused on this region because soil-transmitted helminths are endemic, and their burden is significant in this region. This review was conducted according to the Preferred Reporting Items for Systematic Review and Meta-analysis (PRISMA) checklist. Relevant studies were searched in health-related databases including PubMed, Ovid, and Google Scholar. We selected studies based on the selection criteria set for this review. We collected textual information about the type of health services research, the degree of community engagement, the research phases involved, and the barriers/enablers affecting community engagement in research since they are pertinent to our review question and objective. Ten studies from seven countries in the Asia Pacific region were identified for this review. Albeit with variation in the extent of their involvement, various forms of communities/groups within communities were included such as Aboriginal communities, local communities, school children and their parents, school teachers and headmasters of schools, heads of villages, religious leaders, and so on. Overall, community engagement in health services research focused on of soil-transmitted helminths was limited. Six studies (60%) had collaboration at ‘developing methodology’, mainly through an explanation of the objectives of the study or study process to be conducted. Seven studies (70%) revealed community participation in soil-transmitted helminths at the “data collection” stage. Only one study (10%) documented that a community leader was involved as a co-author, reflecting an involvement in ‘report writing’ and further ‘dissemination’. Findings suggest that there were various forms of community engagement in various aspects of the health services research context. Overall, there was moderate level of participation, but there was insufficient information on the partnership between various stakeholders, which prevented in-depth analysis of the engagement. Future health services research on soil-transmitted helminth interventions needs to be carefully planned, well designed, grounded in principles of community engagement, and designed methodologically to allow in-depth participation by communities in all stages of the research.
New Cretaceous (Aptian/Albian) boxer shrimp (Crustacea, Decapoda, Stenopodidea) from the Araripe Sedimentary Basin, NE, Brazil
Authors: Alencar Damares Ribeiro , Santana William , Pinheiro Allysson Pontes , Lima Daniel , Feitosa Saraiva Antônio Álamo , de Oliveira Gustavo Ribeiro
Published: March 2023

Stenopodidean shrimps are mostly cryptic in their habitats and are typically related with coral rubble or dead coral heads, rocks and crevices, and in association with other marine invertebrate such as sponges, crinoids and corals. Here, we describe a new stenopodidean shrimp, Dubiostenopus parvus n. gen. n. sp., from the Romualdo Formation (Aptian/Albian), Araripe Sedimentary Basin. The specimen studied here was collected in the municipality of Trindade, Pernambuco State, Brazil. The specimen is the imprint of a small shrimp approximately 10 mm in length, with a robust cephalothorax, a well-developed cheliped in the third pereiopod, and a second pleura not overlapping the first. This is the first stenopodidean shrimp described from the Romualdo Formation and the first described from South America. Comparisons with other Brazilian shrimp-like fossils are made, as well as comparisons with all other fossil stenopodideans.
Disrespect and abuse in maternity care in a low-resource setting in Tanzania: Provider’s perspectives of practice
Authors: Mwasha Loveluck K. , Kisaka Lucy M. , Pallangyo Eunice S.
Published: March 2023

Background

Globally, women experience disrespectful and abusive care from maternity healthcare providers at health facilities, committed intentionally or unintentionally, particularly during labor and delivery. Disrespectful care affects women’s childbirth experience and birth outcomes.

Methods

This study used a descriptive qualitative design to obtain thick and rich data on disrespect and abuse in maternity care in a low-resource setting in Tanzania. Three days workshop was conducted at the Aga Khan University comprising maternity healthcare providers from diverse settings. The workshop was designed based on the existing evidence and anecdotal data and inspired by the authors’ experiences of disrespectful and abusive care (stereotyping clients, not listening to client’s/relatives’ concerns, unconsented care) as a client, relative, or observant of colleagues. The targeted audience was maternity healthcare providers from public and private health facilities in the Dar es Salaam region. Data collection encompassed individual responses (reflection of practice) obtained by individuals, anonymously written reflections of practice, and compiled notes from group discussions. Data were analysed thematically guided by six steps described by Braun and Clerk.

Results

A total of 80 maternity healthcare providers participated in the workshop from various health facilities, including dispensaries (n = 25), health centres (n = 2), and hospitals (n = 3) located in semi-urban Dar es Salaam.

Four main themes were identified from the data: Physical and verbal abuse; Lack of professional ethics and integrity; Vulnerable working environment; Abuse and disrespect to care providers. In addition, several sub-themes were identified within these themes: Harsh and abusive language; Beating/slapping/pinching of the mother in labor; notably, Junior midwives also disrespected and abused women; Lack of privacy and confidentiality; Poor communication; No consent for maternity healthcare procedures; Lack of courtesy and poor interpersonal skills; and, negligence of care and woman’s needs.

Conclusion

The actions of disrespect and abuse are alarming in practice and are associated with ignorance of fundamental human rights by both providers and recipients of services. Conducting workshops seems a useful approach to revealing disrespect and abuse deep-rooted in practice and provides an opportunity to rectify the problem with providers. A more extensive interventional study will be crucial to address the widespread actions of disrespect and abuse.
The unintended consequences of tolerance: The experience and repercussions of being tolerated for minority group members
Authors: Adelman Levi , Yogeeswaran Kumar , Verkuyten Maykel
Published: March 2023

Tolerance as forbearance implies the decision to not interfere when others engage in actions or beliefs that you disapprove of. This allows minorities to live the life that they want, despite the disapproval. However, the undercurrent of disapproval which comes with tolerance might carry unintended negative consequences for tolerated minorities. The present research utilizes a novel experimental method to give participants an experience of being tolerated to address two key questions: 1) what are the consequences of being tolerated on personal well-being? 2) how does the experience of being tolerated affect future expectations and willingness to raise one’s voice? Across four studies with American and Dutch participants (N = 1,758), we find that being tolerated leads to less positive outcomes on well-being than being accepted (although more positive implications relative to being rejected). Further, being tolerated reduces the expectation of being valued in future encounters while simultaneously tamping down on people’s willingness to raise their voice against those who begrudgingly include them.
Obstacles to patient inclusion in CPR/DNAR decisions and challenging conversations: A qualitative study with internal medicine physicians in Southern Switzerland
Authors: Bedulli Michele , Falvo Ilaria , Merlani Paolo , Hurst Samia , Fadda Marta
Published: March 2023

Despite cardiopulmonary resuscitation (CPR) and do-not-attempt-resuscitation (DNAR) decisions are increasingly considered an essential component of hospital practice and patient inclusion in these conversations an ethical imperative in most cases, there is evidence that such discussions between physicians and patients/surrogate decision-makers (the person or people providing direction in decision making if a person is unable to make decisions about personal health care, e.g., family members or friends) are often inadequate, excessively delayed, or absent. We conducted a study to qualitatively explore physician-reported CPR/DNAR decision-making approaches and CPR/DNAR conversations with patients hospitalized in the internal medicine wards of the four main hospitals in Ticino, Southern Switzerland. We conducted four focus groups with 19 resident and staff physicians employed in the internal medicine unit of the four public hospitals in Ticino. Questions aimed to elicit participants’ specific experiences in deciding on and discussing CPR/DNAR with patients and their families, the stakeholders (ideally) involved in the discussion, and their responsibilities. We found that participants experienced two main tensions. On the one side, CPR/DNAR decisions were dominated by the belief that patient involvement is often pointless, even though participants favored a shared decision-making approach. On the other, despite aiming at a non-manipulative conversation, participants were aware that most CPR/DNAR conversations are characterized by a nudging communicative approach where the physician gently pushes patients towards his/her recommendation. Participants identified structural cause to the previous two tensions that go beyond the patient-physician relationship. CPR/DNAR decisions are examples of best interests assessments at the end of life. Such assessments represent value judgments that cannot be validly ascertained without patient input. CPR/DNAR conversations should be regarded as complex interventions that need to be thoroughly and regularly taught, in a manner similar to technical interventions.
Multi-faceted metagenomic analysis of spacecraft associated surfaces reveal planetary protection relevant microbial composition
Authors: Highlander Sarah K. , Wood Jason M. , Gillece John D. , Folkerts Megan , Fofanov Viacheslav , Furstenau Tara , Singh Nitin K. , Guan Lisa , Seuylemezian Arman , Benardini James N. , Engelthaler David M. , Venkateswaran Kasthuri , Keim Paul S.
Published: March 2023

The National Aeronautics and Space Administration (NASA) has been monitoring the microbial burden of spacecraft since the 1970’s Viking missions. Originally culture-based and then focused 16S sequencing techniques were used, but we have now applied whole metagenomic sequencing to a variety of cleanroom samples at the Jet Propulsion Lab (JPL), including the Spacecraft Assembly Facility (SAF) with the goals of taxonomic identification and for functional assignment. Our samples included facility pre-filters, cleanroom vacuum debris, and surface wipes. The taxonomic composition was carried out by three different analysis tools to contrast marker, k-mer, and true alignment approaches. Hierarchical clustering analysis of the data separated vacuum particles from other SAF DNA samples. Vacuum particle samples were the most diverse while DNA samples from the ISO (International Standards Organization) compliant facilities and the SAF were the least diverse; all three were dominated by Proteobacteria. Wipe samples had higher diversity and were predominated by Actinobacteria, including human commensals Cutibacterium acnes and Corynebacterium spp. Taxa identified by the three methods were not identical, supporting the use of multiple methods for metagenome characterization. Likewise, functional annotation was performed using multiple methods. Vacuum particles and SAF samples contained strong signals of the tricarboxylic acid cycle and of amino acid biosynthesis, suggesting that many of the identified microorganisms have the ability to grow in nutrient-limited environments. In total, 18 samples generated high quality metagenome assembled genomes (MAG), which were dominated by Moraxella osloensis or Malassezia restricta. One M. osloensis MAG was assembled into a single circular scaffold and gene annotated. This study includes a rigorous quantitative determination of microbial loads and a qualitative dissection of microbial composition. Assembly of multiple specimens led to greater confidence for the identification of particular species and their predicted functional roles.
Reconstructing the geological provenance and long-distance movement of rectangular, fishtail, and croisette copper ingots in Iron Age Zambia and Zimbabwe
Authors: Stephens Jay , Killick David , Chirikure Shadreck
Published: March 2023

The southern third of Africa is unusually rich in copper ore deposits. These were exploited by precolonial populations to manufacture wound-wire bangles, other forms of jewelry, and large copper ingots that were used as stores of copper or as forms of prestige. Rectangular, fishtail, and croisette ingots dating between the 5th and 20th centuries CE have been found in many locations in the Democratic Republic of the Congo (DRC), Zambia, and Zimbabwe, with isolated finds in Malawi and Mozambique. Molds for casting these ingots have been found mostly in the Central African Copperbelt, but also around the Magondi Belt copper deposits in northern Zimbabwe. For years, scholars have debated whether these ingots were exclusively made in the Copperbelt or if the molds found in Zimbabwe indicate that local copies were produced from Magondi Belt copper ore (Garlake 1970; Bisson 1976). Before the recent application of lead isotopic and chemical methods to provenance copper in central and southern Africa, there was no way to discern between these hypotheses. Rademakers et al. (2019) and Stephens et al. (2020) showed that copper artifacts from southern DRC (mostly from Upemba) and from northwestern Botswana (Tsodilo Hills) match the lead isotope ratios of ores from the Copperbelt. Building upon these previous studies, we present here the first results from a copper provenance project across the southern third of Africa, from the Copperbelt to northern South Africa. We apply lead isotopic analysis (LIA) and chemical analyses to establish the provenance of 29 croisette ingots recovered in Zimbabwe, 2 fishtail and 1 rectangular ingot recovered from sites in Zambia, and an “X” shaped ingot smelted in an experiment in Zambia in the 1970’s. Our chemistry and lead isotopic results indicate that 16 of these objects were smelted with copper from the Copperbelt, 16 objects source more specifically to the Kipushi deposit within this geological district, and only one HXR ingot sources to the Magondi Belt in Zimbabwe. Taken together, we clearly illustrate that croisette ingots were traveling significant distances to reach their eventual sites of deposition, and that there was also local production of these objects in Zimbabwe.
Extensible membrane nanotubules mediate attachment of Trypanosoma cruzi epimastigotes under flow
Authors: Perdomo-Gómez Cristhian David , Ruiz-Uribe Nancy E. , González John Mario , Forero-Shelton Manu
Published: March 2023

Trypanosoma cruzi is the etiological agent of Chagas disease, an important cause of infectious chronic myocardiopathy in Latin America. The life cycle of the parasite involves two main hosts: a triatomine (arthropod hematophagous vector) and a mammal. Epimastigotes are flagellated forms inside the triatomine gut; they mature in its intestine into metacyclic trypomastigotes, the infective form for humans. Parasites attach despite the shear stress generated by fluid flow in the intestines of the host, but little is known about the mechanisms that stabilize attachment in these conditions. Here, we describe the effect of varying levels of shear stress on attached T. cruzi epimastigotes using a parallel plate flow chamber. When flow is applied, parasites are partially dragged but maintain a connection to the surface via ~40 nm wide filaments (nanotubules) and the activity of flagella is reduced. When flow stops, parasites return near their original position and flagellar motion resumes. Nanotubule elongation increases with increasing shear stress and is consistent with a model of membrane tether extension under force. Fluorescent probes used to confirm membrane composition also show micron-wide anchoring pads at the distal end of the nanotubules. Multiple tethering accounts for more resistance to large shear stresses and for reduced flagellar movement when flow is stopped. The formation of membrane nanotubules is a possible mechanism to enhance adherence to host cells under shear stress, favoring the continuity of the parasite´s life cycle.
A study on the effect of host plants on Chinese gallnut morphogenesis
Authors: Lu Qin , Chen Hang , Zhang Jinwen , Wang Weiwei , Cui Yongzhong , Liu Juan
Published: March 2023

Galls are products of the hyperplasia of host plant structures induced by gall-inducing organisms and have been considered as an extended phenotype of the inducers. There is little evidence regarding the effect of host plants on gall morphology. We hypothesised that the morphology and developmental pattern of galls are different because of the different location of their stimulation, even though two kinds of inducers are close relatives. We observed that horned galls and their leaflets of their host plant, Rhus chinensis required a longer rapid growth stage than fusiform galls and Rhus potaninii leaflets. The distribution of trichomes showed positional dependence. Molecular analysis showed that in the fusiform gall, the target genes that regulate the plastochron of leaflets and serration development were hardly expressed, and CUP-SHAPED COTYLEDON-2 may be a key gene that regulates the formation of the horns. In summary, horned and fusiform galls showed a developmental pattern similar to those of their host plant leaflets. We suggest that the inducing site is important in the morphology and development of galls.
Gene expression of pro-inflammatory (IL-8, IL-18, TNF-α, and IFN-γ) and anti-inflammatory (IL-10) cytokines in the duodenum of broiler chickens exposed to lipopolysaccharides from Escherichia coli and Bacillus subtilis
Authors: Rodríguez Sandra Paola , Herrera Albeiro López , Parra Jaime Eduardo
Published: March 2023

Background and Aim: Intestinal infections are associated with Gram-negative bacteria like Escherichia coli. When eliminated by treatments during replication, E. coli release lipopolysaccharides (LPS) that can activate the intestinal immune system and increase the expression of cytokines, such as interleukin (IL)-8, IL-18, tumor necrosis factor-alpha (TNF-α), and interferon-gamma (IFN-γ), by the intestinal epithelium under pathological conditions. This study aimed to evaluate the addition of Bacillus subtilis to the duodenal gene expression of pro-inflammatory and anti-inflammatory cytokines in broilers exposed to LPS from E. coli.

Materials and Methods: RNA was extracted using the Zymo Research total RNA commercial kit, according to the manufacturer's specifications, from the intestinal tissue of the duodenum previously resuspended in the lysis buffer of the kit. The expression of the cytokines of interest was measured using the QuantiNova SYBR green real-time polymerase chain reaction kit (Qiagen). Transcript quantification was performed by the ΔΔC(t) method using glyceraldehyde 3-phosphate dehydrogenase as a normalizing constitutive gene.

Results: For the measurement of pro-inflammatory (IL-8, IL-18, TNF-α, and IFN-γ) and anti-inflammatory (IL-10) cytokines, there was no statistically significant difference (p > 0.05) between the basal diet and the diet with antibiotic (avilamycin). There was a statistical difference (p < 0.05) between diets with LPS. The diet with B. subtilis presented the lowest expression; the results differed on each sampling day (days 14, 28, and 42).

Conclusion: A decrease in the expression of pro-inflammatory cytokines (IL-8, IL-18, TNF-α, and IFN-γ) and an increase in IL-10 (anti-inflammatory) was observed; in this way, a balance of the inflammatory response to bacterial infection is achieved, suggesting that the use of B. subtilis as an additive in a broiler diet has a similar effect to that produced with antibiotic growth promoter.

Keywords: antibiotic, enteritis, Escherichia coli, lipopolysaccharides.
Secondary Sclerosing Cholangitis After Emphysematous Cholecystitis
Authors: Takahashi Koji , Ohyama Hiroshi , Takiguchi Yuichi , Sekine Yu , Toyama Shodai , Yamada Nana , Sugihara Chihei , Kan Motoyasu , Ouchi Mayu , Nagashima Hiroki , Iino Yotaro , Kusakabe Yuko , Okitsu Kohichiroh , Ohno Izumi , Kato Naoya
Published: March 2023

A 64-year-old woman was diagnosed with emphysematous cholecystitis. An open cholecystectomy was performed immediately. After the cholecystectomy, jaundice and multiple bile duct strictures that were not present preoperatively appeared. The patient was diagnosed with sclerosing cholangitis secondary to emphysematous cholecystitis. Endoscopic biliary stenting and endoscopic biliary balloon dilatation were performed. However, jaundice did not improve. She developed candidemia 75 days after cholecystectomy. The patient died of multiple organ failures 92 days after cholecystectomy. Although rare, secondary sclerosing cholangitis occurred after emphysematous cholecystitis, and endoscopic treatment was ineffective in this case.
Musiktheater im Legitimationsdiskurs
Authors: Wiese Caroline
Published: March 2023

Im Diskurs um das Musiktheater weitet sich ein Legitimationsproblem aus, das politische, wirtschaftliche und ästhetische Aspekte betrifft und die Kunstform in ihrer Existenz in Frage stellt. Welche Legitimationsstrategien bestimmen aber diesen Diskurs? Caroline Wiese nimmt Musiktheaterkritiken zu exemplarisch gewählten Werken aus den Bereichen Oper, Operette, Neues Musiktheater und Musical in den Fokus. Anhand des Materials fächert sie diskursive Denkmuster und Argumentationsstrukturen auf, die im Umfeld des Dispositivs »Musiktheater« verdichtet auftreten, und zeigt: Unabhängig von Gattung und Werk werden teils widersprüchliche Strategien wirksam.
Arbeit und Gesundheit in der Spätmoderne
Authors: Jelenko Marie
Published: March 2023

Immer schneller, weiter, mehr! Bei diesem leistungsorientierten Diktum sind arbeitsbezogene Gesundheitsgefahren allgegenwärtig. Sozialpolitisch wird deren Vorbeugung in der betrieblichen Prävention fokussiert, die traditionell an der Unfallverhütung und der männlichen Industriearbeit orientiert ist. Im Kontrast dazu steht die Forderung spätmoderner Dienstleistungsgesellschaften nach mehr Flexibilität und Einsatzbereitschaft. Marie Jelenko untersucht, wie die Bewältigung von erhöhten Anforderungen und das damit verbundene Gesundheitsrisiko auf den Schultern des Individuums abgeladen werden.
Gestaltung in virtuellen Welten
Authors: Vogt Benjamin
Published: March 2023

Die Linie fungiert als grundlegendes Kommunikationswerkzeug der menschlichen Raumwahrnehmung, womit ihr auch in virtuellen Welten enorme Bedeutung zukommt. Benjamin Vogt eröffnet durch seine philosophisch-historische Betrachtung dieser Funktionalität nicht nur neue Blickwinkel auf virtuelle Welten, er liefert auch eine passende designtheoretische Grundlegung hierzu. Die virtuelle Realität steht als Darstellungsmedium in der Nachfolge zeichnerischer (geometrischer und geografischer) Darstellungen. Über die Linienwelt erschließt sich die Interaktion in virtuellen Welten als ein immersives Betreten unserer mentalen Kommunikations- und Abstraktionsebene - und lässt die Welt in ihrer Visualisierung aufgehen.
Pinched hypersurfaces are compact
Authors: Bourni Theodora , Langford Mat , Lynch Stephen
Published: March 2023

We make rigorous and old idea of using mean curvature flow to prove a theorem of Richard Hamilton on the compactness of proper hypersurfaces with pinched, bounded curvature.
Trends and forecasted rates of adverse childhood experiences among adults in the United States: an analysis of the Behavioral Risk Factor Surveillance System
Authors: Hartwell Micah , Hendrix-Dicken Amy , Terry Rachel , Schiffmacher Sadie , Conway Lauren , Croff Julie M.
Published: March 2023

Context

Many studies have shown increases in negative social aspects in the United States that may increase the likelihood of a child experiencing adversity. These rising trends include household dysfunction, poor mental health and substance use, crime rates, and incarceration. Additionally, the pathway of adverse childhood experiences (ACEs) may also perpetuate intergenerational trauma.

Objectives

Given these increased trends, our objective was to determine the mean ACEs reported among adults by year of birth to assess trends of ACEs over time.

Methods

To assess ACEs trends in the United States, we utilized data from the 2020 Behavioral Risk Factor Surveillance System (BRFSS), a nationally representative survey. We summed individuals’ reported ACEs and then calculated the mean ACE score within age cohorts (in 1-year increments) by year of birth. We utilized an auto-regressive integrated moving average (ARIMA) model to forecast mean ACEs through 2030.

Results

Respondents to the ACEs module (n=116,378) represented 63,076,717 adults in the United States, with an average age cohort of 1715 individuals. The mean reported ACEs among individuals 80 years or older (born in or before 1940) was 0.79, while the highest mean ACEs (2.74) were reported among the cohort born in 1998—an average increase of 0.022 ACEs per year. The ARIMA model forecasted that individuals born in 2018 will, on average, surpass a cumulative of three ACEs.

Conclusions

Given the connection of ACEs to poor health outcomes and quality of life, this trend is alarming and provides evidence for the necessity of child maltreatment prevention. Multigenerational trauma-informed care and education are warranted for individuals with ACEs and may even prevent the cycle from recurring.
On-chip mode-selective manipulation based on the modal-field redistribution assisted with subwavelength grating structures
Authors: Yi Xiaolin , Li Chenlei , Zhao Weike , Zhang Long , Shi Yaocheng , Dai Daoxin
Published: March 2023

Efficient mode-selective manipulation in multimode photonics has drawn much attention as a key technology for realizing scalable and flexible mode-division multiplexing (MDM) systems. A mode-selective manipulation scheme based on the modal-field redistribution assisted with subwavelength grating (SWG) structures is proposed and demonstrated for the first time. In particular, the proposed scheme focuses on manipulating the coupling coefficient κ as well as the ratio δ/κ for different mode channels. The SWG structures are used to engineer the refractive-index profile and redistribute the modal field distributions in the multimode bus waveguide, so that different modes are localized in different local regions. In this way, the undesired mode coupling can be suppressed significantly while the desired mode coupling can be enhanced. With such mode manipulation scheme, the fundamental and higher-order mode channels in the bus waveguide can be added/dropped independently and freely. As a proof of concept, a three-channel mode-selective add-drop coupler utilizing the proposed scheme is fabricated and demonstrated experimentally on silicon. The fabricated devices show low excess losses ranging from 0.1 to 1.9 dB over a wavelength range of 70 nm. The inter-mode crosstalks are lower than −19.4 dB in the wavelength range of 1525–1600 nm. The crosstalks for the drop and through ports (i.e., the residual power) are suppressed to be as low as −18 ∼ −30 dB in the wavelength range of ∼60 nm with the assistance of an additional coupler in cascade for performance improvement. The present concept of manipulating the evanescent coupling of the mode-channels paves the way for designing multimode silicon photonic devices with flexible mode-selective manipulation for MDM systems.