bluuebunny/crossref_metadata_2025 huggingface dataset:
Possibilidades do uso das Tecnologias da Informação e Comunicação para o Letramento Matemático segundo as diretrizes da Base Nacional Comum Curricular (BNCC)
Authors: Costa Mario Jorge Nunes , Almeida Josiane Marques Duarte , Reis Josiane Silva dos , Santos Maria Jose Costa dos
Published: September 2022

Devido ao baixo desempenho dos estudantes brasileiros em matemática e seu obsoleto ensino, surge a necessidade de propostas pedagógicas que promovam o letramento matemático. Possibilidades para isto estão no uso educacional das Tecnologias da Informação e Comunicação (TIC), com um ensino divertido e significativo. De modo paralelo, a partir de 2018, implanta-se a Base Nacional Comum Curricular (BNCC), a qual orienta o uso de TIC para o ensino de matemática e discute temas de letramento. Visto que a BNCC é assunto de pesquisas, indaga-se o que estes estudos tratam sobre as diretrizes da base para o uso de TIC, para favorecer o letramento matemático? A investigação apresenta suporte teórico nas ideias de letramento matemático da BNCC e nas vias do uso de TIC para auxiliar a aprendizagem de matemática. Metodologicamente, consiste numa pesquisa bibliográfica, segundo Lakatos e Marconi, com dados extraídos de artigos disponíveis no Google acadêmico, publicados entre 2018 e 2021, e mapeados pela Análise Textual Discursiva (ATD), segundo Moraes. Os resultados revelaram que o emprego de tecnologias pode ser favorável para trabalhar competências de letramento do tipo: comunicação, representação, raciocínio e resolução de problemas matemáticos, contudo observou-se a existência de escassos trabalhos teóricos que interrelacionam o uso de TIC e letramento matemático, o que pode representar lacunas entre o uso de tecnologias e o desenvolvimento de práticas pedagógicas ou falta de formação docente adequada para tanto. Para futuros trabalhos, sugere-se investigar formações docentes e experiencias concretas de como empregar tecnologias para trabalhar competências de Letramento Matemático.
Theme Park Imitations: The Case of Happy World (Happy Valley Beijing)
Authors: Carlà-Uhink Filippo , Freitag Florian
Published: September 2022

Theme parks frequently draw not only on historical themes, from antiquity to the roaring twenties, but also on their own history – that is, the history of the medium of the theme park itself. This article uses the example of the Happy World ride at Happy Valley Beijing (China) to discuss theme park imitations, that is, the fact that theme parks frequently borrow individual elements (themes, technologies, visuals, layouts, names) and/or entire units (rides, restaurants, themed areas) from each other. Opened in 2014 in the Greek-themed Aegean Harbour section of Happy Valley Beijing, Happy World may upon first sight look like an almost exact copy of Disney’s ‘it’s a small world’ (opened at Disneyland in California in 1966) but turns out to be, upon closer examination, a complex refunctionalization of central elements of ‘it’s a small world’ that establishes meaningful connections between (ancient) Greece and the city of Beijing via the theme of the Olympic Games: drawing on the origins of ‘it’s a small world’ in the 1964–5 New York World’s Fair and the latter’s motto of ‘Peace through Understanding’, Happy World takes visitors on a journey from the ancient Olympiad to contemporary Beijing (the site of the 2008 Summer and the 2022 Winter Olympic Games) to offer a theme park rendition of the 2008 Olympic torch relay as an homage to ‘the spirit [of peace, respect, and friendship] in the people’s [ sic] of the world’.
A smart nanoplatform for enhanced photo-ferrotherapy of hepatocellular carcinoma
Authors: Tang Longguang , Ling Mingjian , Syeda Madiha Zahra , Sun Rui , He Minghui , Mu Qingchun , Zhu Xiulong , Huang Chunming , Cui Liao
Published: September 2022

Hepatocellular carcinoma (HCC) is the third leading cause of cancer-related deaths worldwide. Emerging therapies, such as ferroptosis mediated cancer therapy and phototherapy, offer new opportunities for HCC treatment. The combination of multiple treatments is often more effective than monotherapy, but many of the current treatments are prone to serious side effects, resulting in a serious decline in patients’ quality of life. Therefore, the combination therapy of tumor in situ controllable activation will improve the efficacy and reduce side effects for precise treatment of tumor. Herein, we synthesized a GSH-activatable nanomedicine to synergize photothermal therapy (PTT) and ferrotherapy. We utilized a near-infrared dye SQ890 as both an iron-chelating and a photothermal converter agent, which was encapsulated with a GSH-sensitive polymer (PLGA-SS-mPEG), to attain the biocompatible SQ890@Fe nanoparticles (NPs). In the tumor microenvironment (TME), SQ890@Fe NPs showed a GSH-activated photothermal effect that could increase the Fenton reaction rate. Meanwhile, the depletion of GSH could further increase ferroptosis effect. In turn, the increasing radical generated by ferrotherapy could impair the formation of heat shock proteins (HSPs) which could amplify PTT effects by limiting the self-protection mechanism. Overall, the intelligent nanomedicine SQ890@Fe NPs combines ferrotherapy and PTT to enhance the efficacy and safety of cancer treatment through the mutual promotion of the two treatment mechanisms, providing a new dimension for tumor combination therapy.
Neuropilin-1 modulates the 3D invasive properties of glioblastoma stem-like cells
Authors: Kerhervé Mathilde , Rosińska Sara , Trillet Kilian , Zeinaty Alya , Feyeux Magalie , Nedellec Steven , Gavard Julie
Published: September 2022

Glioblastoma multiforme (GBM) is a rare, yet devastating, primary brain tumor in adults. Current treatments remain generally ineffective and GBM almost invariably recurs, resulting in median survival of 15 months. This high malignancy sources notably from the resilience and invasive capabilities of tumor cells. Within GBM, exists a population of self-sustaining transformed cells with stem-like properties (GSCs), which are thought to be responsible for tumor initiation, growth, and invasion, as well as recurrence. In the tumor microenvironment, GSCs might be found in the vicinity of brain endothelial cells, which provide a protective habitat. Likewise, these resistant, quiescent GSCs may accumulate in hypoxic zones, away from the perivascular niche, or travel towards the healthy brain parenchyma, by eminently co-opting neuro-vascular tracks. Herein, we established an ex vivo model to explore GSC invasive behavior. We found that patient-derived cells massively invade the collagen matrix. In addition, we described that the glycoprotein Neuropilin-1 (NRP1) contributes to GSC spreading and invasion. Indeed, both RNA interference-mediated silencing and CRISPR-mediated gene editing deletion of NRP1 strongly impaired the 3D invasive properties of patient-derived GSCs and their close localization to the brain blood vessels. Of note, other typical features of GSCs, such as expansion and self-renewal were maintained. From a mechanistic standpoint, this biological effect might rely on the expression of the β3 subunit integrin cell-extracellular matrix adhesive receptor. Our data, therefore, propose a reliable approach to explore invasive properties of patient glioma cells ex vivo and identify NRP1 as a mediator in this malignant process.
Cold-hot nature identification of Chinese herbal medicines based on the similarity of HPLC fingerprints
Authors: Wei Guohui , Jia Ronghao , Kong Zhiyong , Ji Chengjie , Wang Zhenguo
Published: September 2022

The nature theory of Chinese herbal medicines (CHMs) is the core theory of traditional Chinese medicine (TCM). Cold-hot nature is an important part of CHM nature. It is found that the material basis of cold-hot nature is CHM ingredients. To test the scientific hypothesis that “CHMs with similar cold-hot nature should have similar material basis,” we explored an intelligent method for cold-hot nature identification of CHMs based on the feature similarity of CHM ingredients in this work. Sixty one CHMs were selected for cold-hot nature identification. High performance liquid chromatography (HPLC) was used to separate the chemical ingredients of CHMs and extract the feature information of CHM ingredients. A distance metric learning algorithm was then learned to measure the similarity of HPLC fingerprints. With the learned distance metric, cold-hot nature identification scheme (CHNIS) was proposed to build an identification model to evaluate the cold-hot nature of CHMs. A number of experiments were designed to verify the effectiveness and feasibility of the proposed CHNIS model. The total identification accuracy rate of 61 CHMs is 80.3%. The performance of the proposed CHNIS algorithm outperformed that of the compared classical algorithms. The experimental results confirmed our inference that CHMs with similar cold-hot nature had similar composition of substances. The CHNIS model was proved to be effective and feasible.
Comprehensive analysis of a TPX2-related TRHDE-AS1/PKIA ceRNA network involving prognostic signatures in Hepatitis B virus-infected hepatocellular carcinoma
Authors: Li Gaopeng , Wang Zhuangqiang , Chen Dong , Yin Jun , Mo Zhiyuan , Sun Bianyin , Yang Tao , Zhang Xinning , Zhai Zhensheng , Li Yaoxuan , Chen Pinggui , Dai Yunyan , Wang Zhiming , Ma Jun
Published: September 2022

Hepatitis B virus (HBV) infection is a main carcinogenic factor of hepatocellular carcinoma (HCC). TPX2 microtubule nucleation factor is recently recommended as a novel prognostic biomarker in HBV-infected HCC tissues. This study aimed to explore a TPX2-related ceRNA regulatory network in HBV-infected HCC and the potential impact on HCC prognosis. We comprehensively identified 541 differential expressed lncRNAs (DElncRNAs), 37 DEmiRNAs and 439 DEmRNAs from HBV-related TCGA-HCC cohorts in TPX2low and TPX2high groups. Based on their RNA-RNA interaction and expression analysis, four DElncRNAs (TRHDE-AS1, DLX6-AS1, SNHG14, HOXA11-AS), four DEmiRNAs (miR-23b, miR-320a, miR-589, miR-126) and five DEmRNAs (PKIA, PCDHA2, SHCBP1, PRSS16, KIF18A) in HCC tumor vs normal groups were subjected to the hub regulatory networks analysis and further prognostic value analysis. Importantly, the TRHDE-AS1/miR-23b/PKIA ceRNA network was associated with HCC prognosis. Furthermore, cellular location analysis and base-base interaction analysis indicated that the cytoplasmic lncRNA TRHDE-AS1 was regarded as a ceRNA to sponging miR-23b and then regulating PKIA. Interestingly, correlation analysis suggested the expression correlation between TRHDE-AS1 and PKIA in HCC. Finally, we further performed the methylation and immune infiltration analysis to explore the functional process of PKIA in HCC. We proposed a ceRNA regulatory network may help elucidate the mechanism by which TPX2 contributes to the prognosis of HBV-related HCC.
Short-term cryopreservation and thawing have minimal effects on Plasmodium falciparum ex vivo invasion profile
Authors: Thiam Laty G. , Ansah Felix , Niang Makhtar , Awandare Gordon A. , Aniweh Yaw
Published: September 2022

Ex vivo phenotyping of P. falciparum erythrocyte invasion diversity is important in the identification and down selection of potential malaria vaccine targets. However, due to the lack of appropriate laboratory facilities in remote areas of endemic countries, direct processing of P. falciparum clinical isolates is usually not feasible. Here, we investigated the combined effect of short-term cryopreservation and thawing processes on the ex vivo invasion phenotypes of P. falciparum isolates. Ex-vivo or in vitro invasion phenotyping assays were performed with P. falciparum clinical isolates prior to or following culture adaptation, respectively. All isolates were genotyped at Day 0 for parasite clonality. Subsequently, isolates that were successfully culture-adapted were genotyped again at Days 7, 15, 21, and 28-post adaptation. Invasion phenotyping assays were performed in isogenic isolates revived at different time points (3, 6, and 12 months) post-cryopreservation and the resulting data were compared to that from ex-vivo invasion data of matched isogenic parental isolates. We also show that short-term culture adaptation selects for parasite clonality and could be a driving force for variation in invasion phenotypes as compared to ex vivo data where almost all parasite clones of a given isolate are present. Interestingly, our data show little variation in the parasites’ invasion phenotype following short-term cryopreservation. Altogether, our data suggest that short-term cryopreservation of uncultured P. falciparum clinical isolates is a reliable mechanism for storing parasites for future use.
Assessing the individual roles of FII, FV, and FX activity in the thrombin generation process
Authors: Bai Cuicui , Konings Joke , Ninivaggi Marisa , Lancé Marcus , de Laat Bas , de Laat-Kremers Romy
Published: September 2022

Thrombin generation (TG) is known as a physiological approach to assess the hemostatic function. Although it correlates well with thrombosis and bleeding, in the current setup it is not sensitive to the effects of fluctuations in single coagulation factors. We optimized the calibrated automated thrombinography (CAT) method to quantify FII, FV and FX activity within the coagulation system. The CAT assay was fine-tuned for the assessment of FII, FV and FX by diluting the samples in FII-, FV-, or FX-deficient plasma, respectively, and measuring TG. Plasma FII levels correlated linearly with the ETP up to a plasma concentration of 100% FII. FV and FX levels correlated linearly with the peak height up to a plasma level of 2.5% FV and 10% FX, respectively. Sensitized CAT protocols were designed by adding a fixed volume of a pre-diluted patient sample to FII, FV, and FX deficient plasma in TG experiments. This approach makes the TG measurement dependent on the activity of the respective coagulation factor. The ETP or peak height were quantified as readouts for the coagulation factor activity. The intra- and inter-assay variation coefficients varied from 5.0 to 8.6%, and from 3.5 to 5.9%, respectively. Reference values were determined in 120 healthy subjects and the assays were clinically validated in 60 patients undergoing coronary artery bypass grafting (CABG). The sensitized CAT assays revealed that the contribution of FII, FV, and FX to the TG process was reduced after CABG surgery, leading to reduced prothrombin conversion and subsequently, lower TG.
Identification of immune-related hub genes and miRNA-mRNA pairs involved in immune infiltration in human septic cardiomyopathy by bioinformatics analysis
Authors: Li Jingru , Sun Guihu , Ma Haocheng , Wu Xinyu , Li Chaozhong , Ding Peng , Lu Si , Li Yanyan , Yang Ping , Li Chaguo , Yang Jun , Peng Yunzhu , Meng Zhaohui , Wang Luqiao
Published: September 2022

Septic cardiomyopathy (SCM) is a serious complication caused by sepsis that will further exacerbate the patient's prognosis. However, immune-related genes (IRGs) and their molecular mechanism during septic cardiomyopathy are largely unknown. Therefore, our study aims to explore the immune-related hub genes (IRHGs) and immune-related miRNA-mRNA pairs with potential biological regulation in SCM by means of bioinformatics analysis and experimental validation.

Method

Firstly, screen differentially expressed mRNAs (DE-mRNAs) from the dataset GSE79962, and construct a PPI network of DE-mRNAs. Secondly, the hub genes of SCM were identified from the PPI network and the hub genes were overlapped with immune cell marker genes (ICMGs) to further obtain IRHGs in SCM. In addition, receiver operating characteristic (ROC) curve analysis was also performed in this process to determine the disease diagnostic capability of IRHGs. Finally, the crucial miRNA-IRHG regulatory network of IRHGs was predicted and constructed by bioinformatic methods. Real-time quantitative reverse transcription-PCR (qRT-PCR) and dataset GSE72380 were used to validate the expression of the key miRNA-IRHG axis.

Result

The results of immune infiltration showed that neutrophils, Th17 cells, Tfh cells, and central memory cells in SCM had more infiltration than the control group; A total of 2 IRHGs were obtained by crossing the hub gene with the ICMGs, and the IRHGs were validated by dataset and qRT-PCR. Ultimately, we obtained the IRHG in SCM: THBS1. The ROC curve results of THBS1 showed that the area under the curve (AUC) was 0.909. Finally, the miR-222-3p/THBS1 axis regulatory network was constructed.

Conclusion

In summary, we propose that THBS1 may be a key IRHG, and can serve as a biomarker for the diagnosis of SCM; in addition, the immune-related regulatory network miR-222-3p/THBS1 may be involved in the regulation of the pathogenesis of SCM and may serve as a promising candidate for SCM therapy.
Case report: Spontaneous closure of ventricular pseudoaneurysm post-acute myocardial infarction with non-surgical therapy
Authors: Shuai Xinxin , Hu Xiajun , Wei Yumiao
Published: September 2022

Left ventricle (LV) pseudoaneurysm is a rare disorder post-acute myocardial infarction (AMI). Resection or closure of the pseudoaneurysm by surgery is recommended due to the high propensity of pseudoaneurysm rupture while surgery has also high risks. Conservative therapy could be acceptable in small pseudoaneurysms or patients with high surgical risks. Nevertheless, the risk evaluation and grasp of indication are not clear. This case reported an acute cyst-like LV pseudoaneurysm formation post-AMI-induced myocardial free wall rupture (MFWR), and the patient recovered with spontaneous closure of the fissure and shrinkage of the LV pseudoaneurysm through non-surgical therapy. Based on the observations in the echocardiogram, we proposed that intermittent closing of the fissure and interruption of the blood flow between the LV and the pseudoaneurysm due to LV contraction alleviated stress change on the pseudoaneurysm. The narrow fissure, small pseudoaneurysm, and intermittently interrupted blood flow that benefit fissure healing and pseudoaneurysm stabilization could indicate the prognosis of this patient. Drugs like β-blocker that decreased the stress on the pseudoaneurysm also led to the risk reduction of pseudoaneurysm rupture. To our knowledge, this is the first case that reports a spontaneous closure of LV pseudoaneurysm. The size of the fissure and the pseudoaneurysm, as well as the corresponding hemodynamic state, could be valuable to evaluate the risk and prognosis of the pseudoaneurysm. Optimized medical management was also helpful to pseudoaneurysm stabilization.
Deep-learning-based post-processing for probabilistic precipitation forecasting
Authors: Ji Yan , Zhi Xiefei , Ji Luying , Zhang Yingxin , Hao Cui , Peng Ting
Published: September 2022

Ensemble prediction systems (EPSs) serve as a popular technique to provide probabilistic precipitation prediction in short- and medium-range forecasting. However, numerical models still suffer from imperfect configurations associated with data assimilation and physical parameterization, which can lead to systemic bias. Even state-of-the-art models often fail to provide high-quality precipitation forecasting, especially for extreme events. In this study, two deep-learning-based models—a shallow neural network (NN) and a deep NN with convolutional layers (CNN)—were used as alternative post-processing approaches to further improve the probabilistic forecasting of precipitation over China with 1–7 lead days. A popular conventional method—the censored and shifted gamma distribution-based ensemble model output statistics (CSG EMOS)—was used as the baseline. Re-forecasts run using a frozen EPS—Global Ensemble Forecast System version 12—were collected as the raw ensembles spanning from 2000 to 2019. The re-forecast data were generated once per day and consisted of one control run and four perturbed members. We used the calendar year 2018 as the validation period and 2019 as the testing period, and the remaining 18 years of data were used for training. According to the results, in terms of the continuous ranked probability score (CRPS) and the Brier score, the CNN model significantly outperforms the shallow NN model, as well as the CSG EMOS approach and the raw ensemble, especially for heavy or extreme precipitation events (those exceeding 50 mm/day). A remarkable degradation was seen when reducing the size of training samples from 18 years of data to two years. The spatial distribution of the CRPS shows that the stations in central China were better calibrated than those in other regions. With a lead time of 1 day, the CNN model was found to be superior to the other models (in terms of the CRPS) at 74.5% of the study stations. These results indicate that deep NNs can serve as a promising approach to the statistical post-processing of probabilistic precipitation forecasting.
Thyroid-related adverse events induced by immune checkpoint inhibitors
Authors: Chera Alexandra , Stancu Andreea Lucia , Bucur Octavian
Published: September 2022

Immune checkpoint inhibitors, namely anti-CTLA-4, anti-PD-1 and anti-PD-L1 monoclonal antibodies, have emerged in the last decade as a novel form of cancer treatment, promoting increased survival in patients. As they tamper with the immune response in order to destroy malignant cells, a new type of adverse reactions has emerged, known as immune-related adverse events (irAEs), which frequently target the endocrine system, especially the thyroid and hypophysis. Thyroid irAEs include hyperthyroidism, thyrotoxicosis, hypothyroidism and a possibly life-threatening condition known as the “thyroid storm”. Early prediction of occurrence and detection of the thyroid irAEs should be a priority for the clinician, in order to avoid critical situations. Moreover, they are recently considered both a prognostic marker and a means of overseeing treatment response, since they indicate an efficient activation of the immune system. Therefore, a multidisciplinary approach including both oncologists and endocrinologists is recommended when immune checkpoint inhibitors are used in the clinic.
Analysis of detrended fluctuation function derived from continuous glucose monitoring may assist in distinguishing latent autoimmune diabetes in adults from T2DM
Authors: Zhang Liyin , Tian Qi , Guo Keyu , Wu Jieru , Ye Jianan , Ding Zhiyi , Zhou Qin , Huang Gan , Li Xia , Zhou Zhiguang , Yang Lin
Published: September 2022

Background

We aimed to explore the performance of detrended fluctuation function (DFF) in distinguishing patients with latent autoimmune diabetes in adults (LADA) from type 2 diabetes mellitus (T2DM) with glucose data derived from continuous glucose monitoring.

Methods

In total, 71 LADA and 152 T2DM patients were enrolled. Correlations between glucose parameters including time in range (TIR), mean glucose, standard deviation (SD), mean amplitude of glucose excursions (MAGE), coefficient of variation (CV), DFF and fasting and 2-hour postprandial C-peptide (FCP, 2hCP) were analyzed and compared. Receiver operating characteristics curve (ROC) analysis and 10-fold cross-validation were employed to explore and validate the performance of DFF in diabetes classification respectively.

Results

Patients with LADA had a higher mean glucose, lower TIR, greater SD, MAGE and CV than those of T2DM (P<0.001). DFF achieved the strongest correlation with FCP (r = -0.705, P<0.001) as compared with TIR (r = 0.485, P<0.001), mean glucose (r = -0.337, P<0.001), SD (r = -0.645, P<0.001), MAGE (r = -0.663, P<0.001) and CV (r = -0.639, P<0.001). ROC analysis showed that DFF yielded the greatest area under the curve (AUC) of 0.862 (sensitivity: 71.2%, specificity: 84.9%) in differentiating LADA from T2DM as compared with TIR, mean glucose, SD, MAGE and CV (AUC: 0.722, 0.650, 0.800, 0.820 and 0.807, sensitivity: 71.8%, 47.9%, 63.6%, 72.7% and 78.8%, specificity: 67.8%, 83.6%, 80.9%, 80.3% and 72.4%, respectively). The kappa test indicated a good consistency between DFF and the actual diagnosis (kappa = 0.551, P<0.001). Ten-fold cross-validation showed a stable performance of DFF with a mean AUC of 0.863 (sensitivity: 78.8%, specificity: 77.8%) in 10 training sets and a mean AUC of 0.866 (sensitivity: 80.9%, specificity: 84.1%) in 10 test sets.

Conclusions

A more violent glucose fluctuation pattern was marked in patients with LADA than T2DM. We first proposed the possible role of DFF in distinguishing patients with LADA from T2DM in our study population, which may assist in diabetes classification.
Lipid droplet accumulation in β cells in patients with type 2 diabetes is associated with insulin resistance, hyperglycemia and β cell dysfunction involving decreased insulin granules
Authors: Horii Tomomi , Kozawa Junji , Fujita Yukari , Kawata Satoshi , Ozawa Harutoshi , Ishibashi Chisaki , Yoneda Sho , Nammo Takao , Miyagawa Jun-ichiro , Eguchi Hidetoshi , Shimomura Iichiro
Published: September 2022

Background and objective

Pancreatic fat is a form of ectopic fat. Lipid droplets (LDs) are also observed in β cells; however, the pathophysiological significance, especially for β cell function, has not been elucidated. Our aim was to assess LD accumulation in β cells in various stages of glucose intolerance and to clarify its relationship with clinical and histological parameters.

Methods

We examined 42 Japanese patients who underwent pancreatectomy. The BODIPY493/503-positive (BODIPY-positive) area in β cells was measured in pancreatic sections from 32 patients. The insulin granule numbers were counted in an additional 10 patients using electron microscopy.

Results

The BODIPY-positive area in β cells in preexisting type 2 diabetes patients was higher than that in normal glucose tolerance patients (p = 0.031). The BODIPY-positive area in β cells was positively correlated with age (r = 0.45, p = 0.0097), HbA1c (r = 0.38, p = 0.0302), fasting plasma glucose (r = 0.37, p = 0.045), and homeostasis model assessment insulin resistance (r = 0.41, p = 0.049) and negatively correlated with an increase in the C-peptide immunoreactivity level by the glucagon test (r = -0.59, p = 0.018). The ratio of mature insulin granule number to total insulin granule number was reduced in the patients with rich LD accumulation in β cells (p = 0.039).

Conclusions

Type 2 diabetes patients had high LD accumulation in β cells, which was associated with insulin resistance, hyperglycemia, aging and β cell dysfunction involving decreased mature insulin granules.
A harmonic suppression strategy for grid-connected inverters based on quadrature sinewave extractor
Authors: Xia Yan , Feng Zetao , Chen Renzhao , Wu Jie , Huang Qinyuan
Published: September 2022

Grid-connected inverters need to reduce current harmonics as much as possible. After introducing the input signal’s fundamental and main harmonic quadrature components, a discrete state model is created, and the discrete observer design method is used to propose a harmonic extraction algorithm called quadrature sinewave extractor (QSE). The QSE is a stable recursive operator with the advantages of no phase displacement and the ability to eliminate mutual influence between harmonic components. Compared to the widely used proportional multi-resonant controller, QSE can reduce current harmonics and improve system stable performance by using it in the current control of grid-connected inverters. Finally, comparative experiments on a three-phase grid-connected inverter are used to verify the proposed method’s effectiveness.
Occurrence and risk assessment of organotin compounds in the surface water of the upper Yangtze River Estuary
Authors: Dai Qi , Chen Ling , Li Pan , Xia Shengji , Wang Yanyan , Huang Qinghui
Published: September 2022

Organotin compounds (OTCs) are a category of persistent toxic substances with endocrine disrupting effects and may pose a risk to aquatic organisms and human health via water. This study collected 78 surface water samples from the drinking water source in the upper Yangtze River Estuary from July 2018 to November 2019. Out of the nine OTC targets, tributyltin (TBT), dimethyltin (DMT), trimethyltin (TMT), dibutyltin (DBT), and monobutyltin (MBT) were detected from more than 50% of the water samples, with cumulative concentrations of OTCs ranging from 42.5 ± 54.0 to 421 ± 176 ng Sn L−1. The composition and concentrations of OTCs in the stormy months were different from those in other months. The concentrations of OTCs with the ebbing tide exceeded those in the flooding tide and increased when the flooding tide transitioned to the ebbing tide. A pulsed trend in concentrations of OTCs in river water was evident due to the impact of rainfall runoff and tidal currents. Identified sources of OTCs included ship antifouling paints, agricultural pesticides, and plastic waste. TBT in water sources poses a low risk to human health and a risk to the ecosystem, particularly during the stormy months.
Analyzing the barriers to putting corporate financial expropriations to a halt: A structural modeling of the phenomenon
Authors: Fu Huang , Abbass Kashif , Qazi Tehmina Fiaz , Niazi Abdul Aziz Khan , Achim Monica Violeta
Published: September 2022

Dealing with expropriating investors’ money is a demanding issue in the corporate sector. This study aims to unveil the barriers to putting expropriations to halt, extract the structure underlying the relationships among barriers, and classify them. The study design entails a literature survey, primary data collection, modeling of the issue, and analysis. This study uses Interpretive Structural Modeling (ISM) and Matriced Impacts Cruise’s Multiplication Appliquée a UN Classement (MICMAC) to address this issue. The study uses a non-probability-based purposive sampling technique. The data were collected from a panel of experts consisting of 12 chief financial officers recruited from companies listed on the Pakistan Stock Exchange, Pakistan, from July to September 2022. We find that a “complicated legal system” is the most critical barrier to putting expropriations to a halt, followed by barriers such as “flawed hiring” and “lack of media attention.” In addition, the least critical are barriers such as “loopholes in laws,” “ineffective law enforcement,” “politicized accountability,” and “lack of international coordination.” It is an original and valuable study based on real-time data collected from the respondents having hands-on helm of the companies’ financial affairs. This is a seminal practical study for policymakers and other stakeholders to understand the barriers to halting corporate scandals and explain expropriations.
Analyses of community stability and inter-specific associations between a plant species with extremely small populations (Hopea hainanensis) and its associated species
Authors: Zhang Li , Zhang Hai-Li , Chen Yukai , Nizamani Mir Muhammad , Zhou Qin , Su Xiting
Published: September 2022

Hopea hainanensis is a large evergreen tree belonging to the family Dipterocarpaceae. It is currently listed as an endangered species on the International Union for Conservation of Nature’s (IUCN) Red List of Threatened Species. It is found primarily on China’s Hainan Island and northern Vietnam. To protect the plant species with extremely small populations Hopea hainanensis and understand its community stability and the inter-specific associations between Hopea hainanensis and other occurring species, we set up 16 quadrats in the areas where wild Hopea hainanensis populations occurred on Hainan Island to investigate the composition of plant species in the quadrats. We used the M. Godron Community stability index to quantify community stability. Based on the two × two contingency tables, the statistic X2, association coefficient (AC), and Pearson correlation coefficient (PCC), percentage co-occurrence (PC), and OI index (OI) analyze the degree of association between Hopea hainanensis and the dominant species in each layer. The results showed that the relationship between Hopea hainanensis and the dominant species in the community was not significant. In addition, there were both positive and negative associations between Hopea hainanensis and other examined six tree species, positive associations with 13 shrub species, positive associations with two herbs, and negative associations with species of 7 species. The connection between Hopea hainanensis and herbs is more complex than that between trees, shrubs, and the dominant species. M. Godron’s stability analysis showed that the community where Hopea hainanensis occurred is relatively unstable (36.84/63.16). The ecology is somewhat fragile, indicating that the community is still at the early or middle stage of competing with other species for resource utilization. The stability of community structure and function is a suitable environment for Hopea hainanensis and finally achieve the purpose of adequate protection, we should choose species with strong positive associations for protecting and recovering Hopea hainanensis.
Spatiotemporal changes of ecosystem service trade-offs under the influence of forest conservation project in Northeast China
Authors: Jia Guangliang , Dong Yulin , Zhang Siyu , He Xingyuan , Zheng Haifeng , Guo Yujie , Shen Guoqiang , Chen Wei
Published: September 2022

Trade-offs between ecosystem services are measures of the degree to which the changing differences between the individual ecosystem services. Although changes in ecosystem service trade-offs are critical for forest ecosystem management, spatiotemporal changes in trade-offs are currently less studied, especially under the influence of ecological engineering implementation. Therefore this study explored the spatiotemporal changes and drivers of ecosystem service trade-offs in various forest types following the implementation of the natural forest conservation project (NFCP), with the example of the Greater Khingan Mountains. Spatial analyses, and root mean square error (RMSE) were applied to investigate spatiotemporal changes in trade-offs, and geodetector was employed to assess their driving factors. The results indicated that among all ecosystem service pairs from 1990 to 2020, the trade-offs between habitat quality and net primary productivity (NPP), NPP and soil conservation showed a growing trend. However, the trade-offs between habitat quality and water conservation, NPP and water conservation, and soil conservation and water conservation showed a declining trend. The ecosystem service trade-offs of coniferous (0.47) were higher than either broad-leaved (0.37) or shrubs (0.28). Moreover, changes in the ecosystem service trade-offs of all kinds of forests were spatially heterogeneous. The drivers with the greatest explanatory power in coniferous, broad-leaved, and shrubs were land use (22.68%), land use (15.19%), and NDVI (20.63%), respectively. Environmental factors contributed great mean explanatory power (62.27–71.67%) to the trade-offs than anthropogenic activity factors. Therefore, spatiotemporal changes and drivers of trade-offs in different forests should be contemplated when conducting subsequent ecological restoration programs in the future.
Lysosomal acid lipase deficiency: A rare inherited dyslipidemia but potential ubiquitous factor in the development of atherosclerosis and fatty liver disease
Authors: Besler Katrina J. , Blanchard Valentin , Francis Gordon A.
Published: September 2022

Lysosomal acid lipase (LAL), encoded by the gene LIPA, is the sole neutral lipid hydrolase in lysosomes, responsible for cleavage of cholesteryl esters and triglycerides into their component parts. Inherited forms of complete (Wolman Disease, WD) or partial LAL deficiency (cholesteryl ester storage disease, CESD) are fortunately rare. Recently, LAL has been identified as a cardiovascular risk gene in genome-wide association studies, though the directionality of risk conferred remains controversial. It has also been proposed that the low expression and activity of LAL in arterial smooth muscle cells (SMCs) that occurs inherently in nature is a likely determinant of the propensity of SMCs to form the majority of foam cells in atherosclerotic plaque. LAL also likely plays a potential role in fatty liver disease. This review highlights the nature of LAL gene mutations in WD and CESD, the association of LAL with prediction of cardiovascular risk from genome-wide association studies, the importance of relative LAL deficiency in SMC foam cells, and the need to further interrogate the pathophysiological impact and cell type-specific role of enhancing LAL activity as a novel treatment strategy to reduce the development and induce the regression of ischemic cardiovascular disease and fatty liver.
The expression and methylation of PITX genes is associated with the prognosis of head and neck squamous cell carcinoma
Authors: Zhao Yaqiong , Zhao Jie , Zhong Mengmei , Zhang Qian , Yan Fei , Feng Yunzhi , Guo Yue
Published: September 2022

Background: The PITX gene family, comprising PITX1, PITX2, and PITX3, is critical in organogenesis and has been evolutionary conserved in animals. PITX genes are associated with the advanced progression and poor prognosis of multiple cancers. However, the relationship between the PITX genes and head and neck squamous cell carcinoma (HNSC) has not been reported.

Methods: We used data from The Cancer Genome Atlas (TCGA) to analyze the association between PITX mRNA expression and clinicopathological parameters of patients with HNSC. The prognostic value of PITX genes was evaluated using the Kaplan-Meier plotter. Multivariate Cox analysis was used to screen out prognosis-associated genes to identify better prognostic indicators. The potential roles of PITX1 and PITX2 in HNSC prognosis were investigated using the protein-protein interaction (PPI) network, Gene Ontology (GO) analysis, and the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. The correlation between PITX1 and PITX2 expression or methylation and immune cell infiltration was evaluated using the tumor-immune system interaction database (TISIDB). MethSurv was used to identify DNA methylation and its effect on HNSC prognosis.

Results:PITX genes expression was correlated with different cancers. PITX1 and PITX2 expression was lower in the patients with HNSC. In HNSC, PITX1 expression was significantly related to the clinical stage, histologic grade, and N stage, while PITX2 expression was only significantly related to the histologic grade. The high expression of PITX3 was significantly related to the histologic grade, T stage, and N stage. Survival analysis revealed that PITX genes had prognostic value in HNSC, which was supported by multivariate Cox analysis. PPI network and enrichment analysis showed that the genes interacting with PITX1 and PITX2 belonged predominantly to signaling pathways associated with DNA binding and transcription. Of the CpG DNA methylation sites in PITX1 and PITX2, 28 and 22 were related to the prognosis of HNSC, respectively. Additionally, PITX1 and PITX2 expression and methylation was associated with tumor-infiltrating lymphocytes (TILs).

Conclusion: The PITX genes were differentially expressed in patients with HNSC, highlighting their essential role in DNA methylation and tumor-infiltrating immune cell regulation, as well as overall prognostic value in HNSC.
Genome-wide analysis of the FOXA1 transcriptional regulatory network identifies super enhancer associated LncRNAs in tamoxifen resistance
Authors: Zhang Xiulei , Zhang Qian , Liu Guangzhi
Published: September 2022

Breast cancer is the leading cause of death in female cancers, and what’s worse, tamoxifen resistance occurs in almost 30% breast cancer patients and has seriously attenuated the therapeutic effect. It is widely studied that epigenetic regulation has played important role in the development of tamoxifen resistance. FOXA1 is a pioneer transcription factor that can translate epigenetic signature into transcription regulation and also drive genome-wide enhancer reprogramming in breast cancer. However, the chromatin super enhancer landscape orchestrated by FOXA1 and the key downstream targets of the FOXA1 oncogenic network in tamoxifen resistance remain elusive. Through analyzing the FOXA1 ChIP-seq data in tamoxifen sensitive MCF7 and tamoxifen resistant MCF7/TamR cells, we show that the FOXA1 chromatin occupancy is enhanced in both the promoter and enhancer regions, and the recruitment events may be E2 dependent in both MCF7 and MCF7/TamR cells. By integratively analyzing the FOXA1 ChIP-seq data and RNA-seq data of MCF7 and MCF7/TamR cells, we find that the enhanced or reduced FOXA1 chromatin binding densities may synchronize the transcriptional activity in tamoxifen resistance. Besides, we identify 1003 super enhancer associated protein coding genes and five super enhancer associated lncRNAs (ATP1A1−AS1, CASC11, CASC15, KCTD21−AS1, LINC00885) in tamoxifen resistance. By KM survival analysis, we find that high expression level of ATP1A1−AS1 and its sense transcript ATP1A1 indicates favorable clinical outcome among the luminal endocrine treated breast cancer patients. Further coexpression analysis indicates that ATP1A1-AS1 is significantly correlated with ATP1A1, and RT-qPCR results show that they both are downregulated in MCF7/TamR cells. Our study shows that the FOXA1 transcriptional regulatory network may promote the development of tamoxifen resistance, and identifies one super enhancer associated lncRNA ATP1A1-AS1 that may work as promising biomarker or drug target in tamoxifen resistance.
Contraceptive self-injection through routine service delivery: Health worker perspectives from Uganda
Authors: Morozoff Chloe , Cover Jane , Namagembe Allen , Nsangi Damalie , Komunyena Tumusiime Justine , Stout Auroras , Kidwell Drake Jennifer
Published: September 2022

Self-care reproductive health innovations are increasingly valued as practices that enable women to manage their fertility with greater autonomy. While self-care, by definition, takes place beyond the clinic walls, many self-care practices nonetheless require initial or follow up visits to a health worker. Access to self-care hinges on the extent to which health care workers who serve as gatekeepers find the innovation appropriate and practical. Self-injection of subcutaneous depot medroxyprogesterone acetate (DMPA-SC) is being introduced and scaled in many countries. In late 2018, health workers in Uganda began offering self-injection of DMPA-SC in the public sector, and this study examines health workers' views on the acceptability and feasibility of training women to self-inject. We conducted in-person interviews with 120 health workers active in the self-injection program to better understand provider practices, program satisfaction, and their views on feasibility. A subset of 77 health workers participated in in-depth interviews. Quantitative data was analyzed using Stata (v14) software, and chi square and student t tests used to measure between group differences. Qualitative data was analyzed using Atlas.ti, employing an iterative coding process, to identify key themes that resonated. The majority of health workers were very satisfied with the self-injection program and reported it was moderately easy to integrate self-injection training into routine service delivery. They identified lack of time to train clients in the clinic setting, lack of materials among community health workers, and client fear of self-injection as key challenges. Community health workers were less likely to report time challenges and indicated higher levels of satisfaction and greater ease in offering self-injection services. The relatively high acceptability of the self-injection program among health workers is promising; however, strategies to overcome feasibility challenges, such as workload constraints that limit the ability to offer self-injection training, are needed to expand service delivery to more women interested in this new self-care innovation. As self-injection programs are introduced and scaled across settings, there is a need for evidence regarding how self-care innovations can be designed and implemented in ways that are practical for health workers, while optimizing women's successful adoption and use.
miR-150-5p and XIST interaction controls monocyte adherence: Implications for osteoarthritis therapy
Authors: Wang Yu-Han , Tsai Chun-Hao , Liu Shan-Chi , Chen Hsien-Te , Chang Jun-Way , Ko Chih-Yuan , Hsu Chin-Jung , Chang Ting-Kuo , Tang Chih-Hsin
Published: September 2022

Recent literature highlights the importance of microRNAs (miRNAs) functioning as diagnostic biomarkers and therapeutic agents in osteoarthritis (OA) and regulators of gene expression. In OA pathogenesis, cell adhesion molecules (CAMs), especially vascular cell adhesion protein 1 (VCAM-1), recruit monocyte infiltration to inflamed synovial tissues and thus accelerate OA progression. Up until now, little has been known about the regulatory mechanisms between miRNAs, long non-coding RNAs (lncRNAs) and VCAM-1 during OA progression. The evidence in this article emphasizes that the functional feature of miR-150-5p is an interaction with the lncRNA X-inactive specific transcript (XIST), which regulates VCAM-1-dependent monocyte adherence in OA synovial fibroblasts (OASFs). Levels of VCAM-1, CD11b (a monocyte marker) and XIST expression were higher in human synovial tissue samples and OASFs, while levels of miR-150-5p were lower in human OA synovial tissue compared with non-OA specimens. XIST enhanced VCAM-1-dependent monocyte adherence to OASFs. Upregulation of miR-150-5p inhibited the effects of XIST upon monocyte adherence. Administration of miR-150-5p effectively ameliorated OA severity in anterior cruciate ligament transection (ACLT) rats. The interaction of miR-150-5p and XIST regulated VCAM-1-dependent monocyte adherence and attenuated OA progression. Our findings suggest that miR-150-5p is a promising small-molecule therapeutic strategy for OA.
IFN-γ transforms the transcriptomic landscape and triggers myeloid cell hyperresponsiveness to cause lethal lung injury
Authors: Verma Atul K. , McKelvey Michael , Uddin Md Bashir , Palani Sunil , Niu Meng , Bauer Christopher , Shao Shengjun , Sun Keer
Published: September 2022

Acute Respiratory Distress Syndrome (ARDS) is an inflammatory disease that is associated with high mortality but no specific treatment. Our understanding of initial events that trigger ARDS pathogenesis is limited. We have developed a mouse model of inflammatory lung injury by influenza and methicillin-resistant Staphylococcus aureus (MRSA) coinfection plus daily antibiotic therapy. Using this pneumonic ARDS model, here we show that IFN-γ receptor signaling drives inflammatory cytokine storm and lung tissue damage. By single-cell RNA sequencing (scRNA-seq) analysis, we demonstrate that IFN-γ signaling induces a transcriptional shift in airway immune cells, particularly by upregulating macrophage and monocyte expression of genes associated with inflammatory diseases. Further evidence from conditional knockout mouse models reveals that IFN-γ receptor signaling in myeloid cells, particularly CD11c+ mononuclear phagocytes, directly promotes TNF-α hyperproduction and inflammatory lung damage. Collectively, the findings from this study, ranging from cell-intrinsic gene expression to overall disease outcome, demonstrate that influenza-induced IFN-γ triggers myeloid cell hyperresponsiveness to MRSA, thereby leading to excessive inflammatory response and lethal lung damage during coinfection.