bluuebunny/crossref_metadata_2025 huggingface dataset:
PATTERNS OF THE FORMATION OF RURAL PUBLIC BUILDING IN MODERN CONDITIONS OF DEVELOPMENT
Authors: Danchak Ihor , Silnyk Oleksandra
Published: December 2022

The article analyzes the peculiarities of the formation of public buildings in rural settlements and directions of its development. The processes of the formation of the village space, dictated by the factors of culture, society, mentality and traditions over several decades, are highlighted. This architecture is distinguished by a special character filled with natural and climatic features, close to the landscape of the area, accordingly, the optimal consideration of natural factors completely solves most of the operational processes. The public building of the village has a content dictated also by functional features, and its presence gives an opportunity to improve the cultural and household, service side of its residents. The development of rural areas depends to a large extent on the level of provision of social standards at the same time as the availability of housing and energy supply. The relevance of the study is substantiated by the identification of the features of public buildings in rural settlements, their nomenclature and layout, the regularity of arrangement by type of building in terms of the nature of the settlement and the requests of its residents. The research methodology consists of real-time studies of the construction of Ukrainian villages, analysis of the formed planning, geopolitical and social infrastructure, analysis of scientific publications in the field of economics, urbanism and social transformations of rural settlements.
The architecture of the Ukrainian village has its own character and its own aesthetic motives dictated by a number of factors. The history of the village of western Ukraine dates back more than one century, and the layering of experience provides the basis for further development. Public buildings - administrative, cultural and household buildings have an important role in the life of the community, but it is still at the stage of formation. The political and economic situation in the country today dictates new conditions for the full functioning of citizens. Standardization of public buildings in the village is currently imperfect. The insufficient number of studies and the rapid pace of transformation of the urban planning system provide space for further work on the analysis of existing buildings and their renovation.
The study of the existing infrastructure and the needs of the population highlights clear directions for the improvement of the rural environment by minimizing costs in construction and further operation of buildings. Administrative and cultural and household institutions need space compaction due to the multi-functionality of the environment, taking into account current operational needs. The situation that has developed in Ukraine dictates a number of requirements for the conversion of educational and medical institutions in order to increase the level of safety and service of various population groups, which requires the search for effective solutions also in the design of new buildings.
MODELING OF CROSS-SECTIONS OF WOODEN ELEMENTS FOR DIFFERENT OPTIONS AND PERCENTAGES OF REINFORCEMENT
Authors: Gomon Petro
Published: December 2022

A detailed analysis of literary sources on the study of strengthening of wooden elements by various methods was carried out.
The calculation sequence of wooden elements reinforced with glued metal elements is proposed. An algorithm for determining the "moment-curvature" graph using optimized wood deformation diagrams is proposed. Calculations were carried out for various forms of beam cross-sections. Calculations of the cross-section were carried out and "moment-curvature" graphs were determined for different options for the reinforcement of the wooden element (with the reinforcement of the compressed zone, the stretched zone, as well as with the reinforcement of both zones). Schedules were determined with different percentages and methods of reinforcing elements. It was established that the best result of reinforcement occurs when reinforcing compressed and stretched zones, less effective when reinforcing a beam in a compressed zone, the smallest effect of reinforcing a stretched zone.
To simulate the work of reinforced wood, the mechanical characteristics of "pine" wood with a moisture content of 12% and metal, namely the characteristics of A400S reinforcement, were used. Moment-curvature plots are given for cross-sections with different percentages of reinforcement and reinforcement in compression and tension zones. An increase in the load-bearing capacity of a bending wooden element due to various options and percentages of reinforcement reinforcement is presented.
It is necessary to note the change in the shape of the "moment-curvature" graph with symmetrical reinforcement, namely, with an increase in the percentage of reinforcement, its shape approaches a straight line. Based on the analysis, conclusions were drawn. The volume of further experimental and theoretical research on this issue is given.
SELECTION OF FACTORS AFFECTING THE LABOR-INTENSIVENESS OF THE PROCESS OF INSTALLATION OF STRUCTURAL PLATES COVERING SINGLE-STORY BUILDINGS
Authors: Sobko Yurii , Novak Yevheniia
Published: December 2022

Theoretical provisions on the formation of rational structural and technological solutions for the installation and dismantling of structural slabs with simultaneous assembly and disassembly of columns allow to improve the quality of the educational process by introducing it into the educational process.
In accordance with the developed research methodology, the assumption that there should be structural and technological solutions for the installation of structural plates, which will meet the following criteria: accelerated speed of construction of single-story buildings, minimal labor intensity, increased reliability and accuracy, technical and technological possibility as an installation of structural plates, is accepted as a working hypothesis. slabs, as well as the dismantling of buildings without damaging the structures while preserving the possibility of their further use.
The ranking of options for structural and technological solutions for the installation of structural plates was carried out according to efficiency criteria, taking into account the influence of factors according to the expert evaluation method according to the criteria of labor intensity, duration and cost of the process when assessing the reliability and quality of the processes. The technological process was considered in a full cycle, starting from factory production, transport operations, and ending with the erection of all structures in the volume determined by the task.
The structure of the factors was considered in order to select them for further research. In accordance with the given methodology of numerical standardization of the labor intensity and duration of processes, the main factors were those that affect the number of actions, taking into account the movement of installers to certain distances, and those that affect the responsibility for the accuracy and reliability of the positioning of the mounted elements.
A importância da educação financeira no âmbito escolar
Authors: Cardoso Barbara da Silva
Published: December 2022

Em uma sociedade onde o consumo excessivo e indiscriminado é enfatizado, questões relacionadas à alfabetização financeira, consumo consciente, poupança e conceitos de investimento precisam ser discutidas em conjunto nas etapas iniciais da educação básica. Nesse sentido, a escola desempenha um papel fundamental na formação do cidadão, capacitando-o a compreender criticamente o mundo que o cerca e a tomar decisões conscientes, tornando-o ativo na sociedade. Desta forma, este artigo visa refletir sobre a importância da educação financeira no âmbito escolar. O mecanismo utilizado para tal objetivo foi a revisão de literatura. Através do apanhado foi possível observar que os benefícios da educação financeira incluem: melhor qualidade de gastos, menos estresse, maior valor para coisas materiais, uma vida mais equilibrada, autoestima pessoal, futuro melhor, bom plano e melhor experiência.
Defragilization of fragile states: evaluation through a logit regression analysis
Authors: Nascimento Mario Brasil do , Medeiros Sabrina Evangelista
Published: December 2022

The studies about fragile states in the international relations begins around the 1990s, and the issue remains active. According to the World Bank, in 2016, 1.8 billion people lived in fragile states. The projection shows that, in 2050, almost 10% of the global population will live in fragile contexts. Poverty, famine, child mortality, and violence denote some characteristics of state’ fragility. Many fragile states have been sanctuaries for terrorist groups, threatening global security. There are several indications of causes for fragility in the states, however, there is no universal consensus on this. International organizations, such as the Organization for Economic Co-operation and Development (OECD), find a way to tackle the fragility of states. The problem is: what are the significant variables to justify the defragilization of fragile states, once statistics show that, while one group of states in the world remains fragile or even deteriorated, another group is getting better gradually? The goal of this article is to present the results of research and the outcomes of logit regression analysis, of a group of states, from 2007-2016., which got defragilization. The analysis took into consideration 1,473 relevant observations involving 13 supposed explanatory variables. Some independent variables, such as international aid or investments on education, would appear to be significant, however, they were not. Conversely, the results were consistent with the improvement of state governance and reduction of infant mortality. The study opens an avenue for new researches to defragilization processes.
Factors influencing consumer choice: a study of apparel and sustainable cues from Canadian and Indian consumers’ perspectives
Authors: Rahman Osmud , Fung Benjamin C. M. , Kharb Devender
Published: December 2022

The purpose of this study is to investigate the salient effects of product evaluative cues from a cross-national perspective. A web-based survey consisted of eight measuring items of environmental commitment and behaviour, 20 items of product cues, and demographic and behavioural questions were employed. A total of 321 and 309 usable surveys were collected from Canada and India, respectively. The results revealed that Canadian and Indian consumers were more concerned about psychic/aesthetic and physical/functional cues than extrinsic and sustainable cues. In particular, fit and comfort were perceived as the two most important evaluative criteria by both samples. Indian consumers relied more heavily on ‘no child labour’ and ‘no animal skin usage’ cues to evaluate apparel products than their Canadian counterparts. The findings underpin several implications, including aesthetic longevity, versatility, durability, and affordability of clothing, as well as transparency on sustainable production and practices.
The Emergence of Colistin- and Imipenem-Associated Multidrug Resistance in Escherichia coli Isolates from Retail Meat
Authors: Iweriebor Benson Chuks , Egbule Olivia Sochi , Obi Larry C.
Published: December 2022

To determine the prevalence of Escherichia coli and their drug resistance profiles in fresh pork sold at two retail outlets (open-air market and closed retail stores) in Alice, South Africa. Retail meat samples (n = 176) collected from four shops (two from open-air markets and two from closed stores) were analyzed by conventional biochemical and PCR-based molecular confirmatory tests. The confirmed isolates were profiled for antimicrobial susceptibility to a panel of 12 commercial antibiotics: tetracycline, ampicillin, sulphamethoxazole trimethoprim, erythromycin, gentamycin, colistin sulphate, cefotaxime, chloramphenicol, norfloxacin, ciprofloxacin, cefuroxime, and imipenem. Colistin, ampicillin, and erythromycin resistance genes were profiled with the gene-specific primers. Multidrug resistance (MDR) and the association of imipenem and colistin in the MDR profile were determined. A total of 68 (39.08%) E. coli isolates were confirmed by PCR analysis. Resistance was most common to erythromycin (100%), followed by cefotaxime (95.58%), ampicillin (88.23%), cefuroxime (88.23%), trimethoprim-sulphamethoxazole (88.23%), and tetracycline (60.29%). Overall, 27/68 (39.70%) were MDR (≥ 3antibiotics classes). MDR E. coli isolates associated with imipenem resistance (50.00%) and colistin resistance (33.82%) were detected. The resistance genes were detected among the isolates though not in all the phenotypically resistant isolates. The detection of colistin resistance among MDR E. coli isolates from retail meat is troubling as the drug is a last resort antibiotic. Overall, the epidemiological implications of the findings are of public health importance.
Penggunaan Media YouTube dalam Meningkatkan Keterampilan Mendengar Bahasa Arab Kelas X MA Al-Ikhlas Labunti Raha Sulawesi Tenggara
Authors: Jabbar Mujahid Abdul , Kahar Fitrayani , Wahyudin Wahyudin
Published: December 2022

This research demonstrates the use of YouTube media in improving Arabic listening skills at MA AL-Ikhlas Labunti Raha School, Southeast Sulawesi. The purpose of this study was to determine the use of YouTube media in improving Arabic listening skills in MA AL-Ikhlas Labunti Raha, Southeast Sulawesi, dan to facilitate students' learning of Arabic through educational videos on YouTube because it can be accessed anytime anywhere. This Type Of Research is qualitative research that seeks to describe and explore data naturally and comprehensively, in addition to providing an in-depth explanation or evaluation and following developments based on the problems posed. The data collection techniques used are observation dan residence interviews. The data analysis technique used in this study is to collect three types of data, which are qualitative data in the form of observational data, interviews, and documentation. The data will be analyzed qualitatively, which means that all the data will be explained in depth based on the research problem. The results of this study indicate that the YouTube method in Arabic subjects can improve the listening skills of class 10 students at MA AL-Ikhlas Labunti Raha, Southeast Sulawesi. Qualitative evidence of documentation from observations, interviews, and documentation of class 10 MA students at Fidelity Labunti Raha, Southeast Sulawesi.
معالجات مقترحة في الإطار القانوني لاسترداد الأموال الناتجة من الفساد "دراسة في القانون الليبي"
Authors: Amhimmid Abdulbari Almabrouk - , Negasi Mohamed Ibrahim , Al-Toumi Ali Makzoum Mohammed
Published: December 2022

الملخص

       يهدف البحث إلى دراسة النظام القانوني لاسترداد أموال الدولة الناتجة من جرائم الفساد في ليبيا من جانبى القانون المنظم والجهة المختصة، وتأتي الأهمية من الحاجة الملحة لاسترداد الأموال التي نتجت من فساد بحجم ضخم في ليبيا وفق ما أشارت تقارير محلية ودولية، إضافة إلى أهمية مرحلة الاسترداد التي تعكس مدى اهتمام المشرع والجهات المختصة بحماية الأموال العامة باستعادة تلك الأموال، الأمر الذي يشكل رادع لمن يسعى للكسب غير المشروع وتُثنيه عن هذا الفعل المجرّم.

      توصل البحث إلى وجود عدد من القوانين المختصة بحماية الأموال العامة، وعدم وجود قانون موحد ينظم استرداد الأموال من جرائم الفساد، بل مجموعة نصوص قانونية مبعثرة بين عدد من القوانين التي تتضمن حماية المال العام، كذلك توصل البحث إلى أن الجهات المختصة بالاسترداد متعددة بموجب القوانين، منها جهات قضائية وجهات رقابية تابعة للسلطة التشريعية وكذلك بعض الجهات التابعة للسلطة التنفيذية، ومن هذه النتائج تم إبداء بعض الملاحظات كحلول أساسية مقترحة تهدف لإعادة صياغة قانون ينظم حماية المال العام ويفصّل آليات استرداده، ثم يعيد تشكيل جهة عليا تتبع السلطة التنفيذية تتكون من موظفين متنوعي الاختصاصات، ضبطية وقضائية ومحاسبية وقانونية، ولديهم إمكانيات لغة وخلفيات قانونية لأنظمة الدول التي تهرب إليها الأموال، حتى تواكب حاجة نظام الاسترداد متعدد المراحل والإجراءات، وتساعد هذه الجهة على تنسيق إجراء استرداد الأموال في داخل الدولة وخارجها.

الكلمات المفتاحية: استرداد، الأموال، القانون، ليبيا
Implementasi Digital Marketing Berbasis Wordpress pada Ichi Hydroponic Store Madiun
Authors: Cahyo Okky Aldhy Try , Setiawan Dimas , Mei Lenawati
Published: December 2022

Currently, Ichi Hydroponic Store products have been marketing its products using digital marketing through WhatsApp social media. Because product marketing is only through WhatsApp, the promotions carried out by the Ichi Hydroponic Store are only aimed at customers who save WhatsApp numbers. Ichi Hydroponic Store, the researchers developed WordPress as a means of marketing Ichi Hydroponic Store products to reach a wider market. The approach in this study uses the Waterfall model. The waterfall model is a classical model that is systematic and sequential in building software. The name of this model is the Linear Sequential Model. This model is often called the classic life cycle or the waterfall method. The Usability Test results show that the web design is good and can be accepted by the user. The implementation of digital marketing based on WordPress provides an increase in revenue of 30.97% from the comparison ten days before and after the web release. The website is expected to be used properly, and the owner can operate it himself.
Kualitas Pelayanan Badan Penyelenggara Jaminan Sosial Kesehatan Perspektif Undang-Undang Nomor 24 Tahun 2011 (Studi Di Kota Madiun)
Authors: Sarjiyati  , Sukarjono Bambang , Purwati Yuni , Rosada Yuan Salsabilla
Published: December 2022

Abstract--This study aims to determine the quality of health services obtained by users of the Social Security Administering Body according to the perspective of Law Number 24 of 2011 and the efforts that must be made to improve the quality of these services. This study uses a type of empirical juridical research that is based on normative law, but observes how the interaction occurs when the norm works in society. With this research, it is expected to be one of the considerations for the government in making regulations to improve the quality of health insurance in Indonesia as well as, as a reference in improving the quality of services, especially by the Madiun City Health Social Security Organizing Agency.
Rancang Bangun Content Management System Wedding Invitation Berbasis Web Multimedia Menggunakan Metode Object Oriented Hypermedia Design
Authors: Rostilawati Detila , Kurniadi Dede , Setiawan Ridwan
Published: December 2022

Undangan digital menjadi trend yang diminati, salah satunya adalah undangan pernikahan. Undangan digital diminati karena kemudahan penyebarannya menggunakan media sosial tanpa perlu mengantarkannya ke rumah masing-masing yang diundang. Content management wedding invitation bertujuan untuk dapat membantu pengguna dalam pengelolaan undangan pernikahan sehingga menjadi lebih mudah dengan kustomisasi undangan dan penyebaran undangan yang cepat dapat mengurangi penggunaan kertas dan membantu dalam pengelolaan keuangan admin. Penelitian ini menggunakan metode Object-Oriented Hypermedia Design Method (OOHDM) dengan menggunakan tahapan – tahapan yang digunakan dalam membangun content management system wedding invitation berbasis web multimedia yaitu tahapan domain analysis, navigational design, abstract interface design, dan implementation. Hasil yang diperoleh berupa content management system yang dapat membantu pengguna dalam membuat dan mengelola serta mendesain undangan pernikahan sesuai dengan keinginannya, dapat membantu admin dalam mengelola keuangan, mencetak laporan keuangan secara otomatis dan juga membantu pengguna untuk membuat atau mengelola undangan. Dengan menggunakan content management system wedding invitation diperoleh hasil teknologi yang dapat berkontribusi dalam membantu suatu kegiatan penyediaan informasi dalam cakupan undangan pernikahan menjadi lebih modular dan reusable, membantu pengguna dalam proses pengelolaan undangan pernikahan, melakukan kustomisasi tampilan sesuai keinginan, membantu admin atau pengelola web dalam pengelolaan laporan keuangan dengan menghemat waktu dalam melakukan pencarian data keuangan berdasarkan tanggal.
The Form of Solutions and Periodic Nature for Some System of Difference Equations
Authors: ELSAYED Elsayed , AL-JUAİD J.
Published: December 2022

In this paper, we study the form of the solution of the following systems of difference equations of order two w_{n+1}=\frac{w_{n}s_{n-1}}{w_{n}+s_{n-1}},~~~ s_{n+1}=\frac{s_{n}w_{n-1}}{\pm s_{n}\pm w_{n-1}},with nonzero real numbers initial conditions.
Genetics in non-alcoholic fatty liver disease: The role of risk alleles through the lens of immune response
Authors: Sookoian Silvia , Pirola Carlos J.
Published: December 2022

The knowledge on the genetic component of non-alcoholic fatty liver disease (NAFLD) has grown exponentially over the last 10 to 15 years. This review summarizes the current evidence and the latest developments in the genetics of NAFLD and non-alcoholic steatohepatitis (NASH) from the immune system’s perspective. Activation of innate and or adaptive immune response is an essential driver of NAFLD disease severity and progression. Lipid and immune pathways are crucial in the pathophysiology of NAFLD and NASH. Here, we highlight novel applications of genomic techniques, including single-cell sequencing and the genetics of gene expression, to elucidate the potential involvement of NAFLD/NASH-risk alleles in modulating immune system cells. Together, our focus is to provide an overview of the potential involvement of the NAFLD/NASH-related risk variants in mediating the immune-driven liver disease severity and diverse systemic pleiotropic effects.
Identification of high-risk subjects in nonalcoholic fatty liver disease
Authors: Stern Christiane , Castera Laurent
Published: December 2022

Non-alcoholic fatty liver disease (NAFLD) is becoming the most common liver disease worldwide, and its burden is expected to increase due to the growing epidemic of obesity and diabetes. The key challenge among NAFLD patients is to identify those with advanced fibrosis (F3F4), who are at high risk of developing complications and will benefit from specialized management and treatment with new pharmacotherapies when they are approved. Liver biopsy appears unrealistic and unsuitable in practice, given the large number of high-risk patients and its well-known limitations. Non-invasive sequential algorithms using fibrosis-4 index as first-line test, followed by vibration-controlled transient elastography or patented blood test, are the best strategy for case finding of high-risk subjects. In fact, they are now recommended by several international guidelines, and should be used and disseminated to increase awareness among physicians beyond liver clinics where most NAFLD patients are seen.
Pengembangan E-Comic sebagai Media Pembelajaran Matematika Kelas IV SD pada Materi Pecahan
Authors: Soleh Ibnu , Agustin Ririn Dwi
Published: December 2022

This study aims to produce E-Comic Math learning media for the fourth grade of SD Negeri Dadapan 02 Wajak on Fractions. The model used in this E-Comic development research is the 4-D model, namely Define, Design, Develop, and Disseminate. Data collection techniques used in this development research were validation questionnaires from material experts, media experts, and mathematics teachers, as well as student response questionnaires. The average percentage result of validation by material experts reached 89,21% which was categorized as “very feasible”. E-Comic learning media is worth trying out. The average percentage of validation results from media experts reached 86,17% which was categorized as "very feasible". The results of the math teacher validation show that the percentage of the feasibility of E-Comic reaches 87,95% which is categorized as "very feasible". The data on the test results of students' responses to the attractiveness of the E-Comic media, the average result reached 85,83% which was categorized as "very interesting". Thus the results of the development of E-Comic learning media have met the eligibility and attractiveness criteria so that the media is very suitable for use.
Pengembangan E-Modul Berbasis Outdoor Education untuk Meningkatkan Kemampuan Literasi Sains Siswa Kelas X SMA/MA pada Materi Ekosistem
Authors: Maziyah Atik , Pangestuti Ardian Anjar
Published: December 2022

Starting in the 21st century, the 21st century life skills are empowered which has four principles, each of which contains specific skills that need to be empowered in learning activities, such as critical thinking skills, problem solving, metacognition, communication skills, collaboration, innovation and creation, literacy. information, and various other skills (Zubaidah, 2017). As for interactive learning media that can be developed, namely by utilizing interactive multimedia results from Flip PDF Corporate Edition, in the form of E-Modules. This study aims to increase students' motivation to learn the material in a happy and effective atmosphere to encourage students to think critically and improve students' scientific literacy. This type of research is development research, with the ADDIE model (Analysis, Design, Develop, Implement, Evaluate). However, this research stopped at the Develop stage, while the Implement and Evaluate stages could not be carried out due to time constraints. As for the results of the research that has been carried out, it was found that the Outdoor Education-Based E-Module to Improve Science Literacy Skills in the Ecosystem Class X SMA/MA material met the eligibility requirements of 90.6% from the material aspect with very good criteria, 95.7% feasible from the technical aspect. media with very good criteria and also get a response from teachers with very good criteria with a feasibility level of 96.67%. Based on these data, it can be concluded that the E-Module Learning Media Based on Outdoor Education to Improve Science Literacy Skills in the Ecosystem Material for Class X SMA/MA is very suitable for use in learning.
Describing Changes to Abortion Clinical Practices in Response to the Covid-19 Pandemic: A Regional-Based Analysis
Authors:   Department of Obstetrics, Gynecology, and Reproductive Health. Rutgers New Jersey Medical School, Newark, NJ, USA, Lang Madeline , Patel Rushi ,   Department of Obstetrics, Gynecology, and Reproductive Health. Rutgers New Jersey Medical School, Newark, NJ, USA, Malhotra Radhika ,   Department of Obstetrics, Gynecology, and Reproductive Health. Rutgers New Jersey Medical School, Newark, NJ, USA, Larios Karla ,   Department of Obstetrics, Gynecology, and Reproductive Health. Rutgers New Jersey Medical School, Newark, NJ, USA, Lacue Amanda ,   Department of Obstetrics, Gynecology, and Reproductive Health. Rutgers New Jersey Medical School, Newark, NJ, USA, M. Brandi Kristyn ,   Department of Obstetrics, Gynecology, and Reproductive Health. Rutgers New Jersey Medical School, Newark, NJ, USA
Published: December 2022

Introduction: During the pandemic, every state was at liberty to determine if abortion services were an essential or non-essential service. This furthered disparities in resource availability based on location. Our study analyzes changes in abortion care practices as a response to COVID-19 on a regional basis.
Dopamine Detox in Realm of Habits and Behavior
Authors:   Associate professor Medicine, Bahawalpur Medical and Dental College, Bahawalpur, Pakistan., Ahmad Alamgir Muhammad
Published: December 2022

Since the beginning of human evolution, our brain developed its reward and punishment center. The human brain started to release an integral feel–good hormone Dopamine.
Role of Epidemiology in the Development of Public Policies in the 21st Century
Authors:   Venezuelan Public Health Society., Stangl J Mandl
Published: December 2022

Epidemics have become more frequent, more complex and more difficult to prevent and control with the rapid evolution of ecology, urbanisation, climate change, increased travel and fragile public health systems.
Adverse Reactions with Covid-19 Vaccine Booster are of Milder Gravity than with First and Second Dose
Authors:   Specialist in Family and Community Medicine Health Center Santa Maria de Benquerencia. Regional Health Service of Castilla la Mancha (SESCAM), Toledo, Spain., Luis Turabian Jose
Published: December 2022

Background: It is not clearly known whether the third doses (booster) of the covid-19 vaccine present more or fewer adverse reactions than those of the first and second doses.
Acute Kidney Injury in Tropical Countries
Authors: Dhanapriya Jeyachandran , Dineshkumar Thanigachalam , Sakthirajan Ramanathan , Gopalakrishnan Natarajan
Published: December 2022

Acute kidney injury (AKI) in tropical countries is strikingly different from that in countries with a temperate climate. Tropical regions are characterised by year-round high temperatures and the absence of frost, which supports the propagation of infections that can potentially cause AKI. The aetiology and presentation of AKI reflects the ethnicity, socioeconomic factors, and ecological conditions in tropical countries. Apart from infections, other causes of AKI include exposure to animal toxins, ingestion of plant toxins or chemicals, poisoning, and obstetric complications. The low income status, poor access to treatment, and sociocultural practices (use of indigenous medicines) contribute to poor outcomes of patients with AKI. The exact aetiologic diagnosis often cannot be made due to lack of appropriate laboratory services. The epidemiology of AKI in tropical regions is changing over time. Renal replacement therapy is inaccessible to the majority and late presentation with delayed treatment add to the risk for future development of chronic kidney disease. AKI is often the primary cause of chronic kidney disease in the developing world, which increases demand for renal replacement therapy and transplantation. Most causes of AKI in developing countries are preventable and strategies to improve the public health and increased access to effective medical care are the need of the hour. This review offers comprehensive ideas about epidemiology, aetio-pathogenesis, clinical presentation, diagnosis, treatment, and prevention of community-acquired AKI in the tropics, with special reference to the Indian subcontinent. AKI is an under-recognised cause of morbidity and mortality in developing countries and even small, simple interventions could have an impact on its outcome.
Evaluasi Kualitas Sistem Informasi Akademik Dengan Webqual dan IPA
Authors: Jumaryadi Yuwan , Fajri Misbahul , Priambodo Bagus
Published: December 2022

Peran dari teknologi informasi di suatu institusi sangat dibutuhkan untuk menunjang kinerja karyawan di institusi tersebut. Penggunaan teknologi informasi akan meningkatkan tingkat efisiensi dan eketivitas dari suatu proses. Evaluasi terhadap usabilitas merupakan hal yang penting dalam memberikan rekomendasi terhadap sistem informasi untuk meningkatkan kepuasan pengguna. Penelitian ini melakukan evaluasi terhadap kualitas sistem informasi akademik di salah satu perguruan tinggi di Jakarta menggunakan menggunakan metode Website Quality (WebQual) dan Importance Performance Analysis (IPA). Kuadran I merupakan kuadran dengan prioritas penting, dimana indikator pada kuadran I adalah Usability_2. Pada Kuadran II sistem informasi akademik harus mempertahankan indikator yang ada seperti Inf_Qual_1, Inf_Qual_2, Inf_Qual_3, Inf_Qual_4, Usability_1, Usability_3, dan Usability_4. Pada kuadran III merupakan indikator yang dapat diabaikan kepentingannya, dimana indikator yang terdapat didalamnya adalah Serv_Int_4, Serv_Int_3, Inf_Qual_5, Usability_5, dan Usability_6, dan kuadran IV dinilai memiliki tingkat kepentingan yang rendah namun memiliki tingkat kinerja yang sangat baik dimana indikatornya adalah Serv_Int_1, Serv_Int_2, Usability_7. Berdasarkan penelitian yang dilakukan, maka indikator Usability_2 merupakan hail prioritas agar dapat ditingkatkan untuk meningkatkan kepuasan pengguna terhadap penggunaan sistem informasi akademik.
REVİZE EDİLMİŞ EBEVEYNLİK DAVRANIŞI ENVANTERİ’NİN (R-EDE) TÜRK KÜLTÜRÜNE UYARLANMASI: BİR GEÇERLİK VE GÜVENİRLİK ÇALIŞMASI
Authors: UZUN CİCEK Ayla , SARIÇAM Hakan
Published: December 2022

Bu çalışmanın amacı, Revize Edilmiş Ebeveynlik Davranışı Envanteri'nin (R-EDE) Türkçe versiyonunun geçerlik ve güvenirliğini incelemektir. Araştırmaya Kütahya, Sivas ve Uşak illerinden 1049 ebeveyn katılmıştır. Ölçeğin psikometrik özellikleri test tekrar test, Cronbach alfa, açıklayıcı faktör analizi, doğrulayıcı faktör analizi ve eşzamanlı ölçek geçerliği yöntemleri ile incelenmiştir. Ölçeğin ölçüt bağlantılı geçerliliğini saptamak için Aile Uyum Ölçeği-Kısa Formu ve Aile Hayatı ve Çocuk Yetiştirme Tutumu Ölçeği kullanılmıştır. Doğrulayıcı faktör analizi sonuçlarına göre, üç boyutlu modelin (Sıcaklık, Kontrol, Düşmanlık/Aşırı kızgınlık) uyum iyiliği değerleri [CMIN=1244.94, sd=402 CMIN/sd =3.097; (p=.00), RMSEA=.083, CFI=.89, GFI=.88, TLI=.84, SRMR=.076] kabul edilebilir bir uyum göstermiştir. Ayrıca, madde faktör yükleri 33 ila .88 arasında değişmektedir. R-EDE ile Aile Uyum Ölçeği ve Aile Hayatı ve Çocuk Yetiştirme Tutumu Ölçeği arasında istatistiksel olarak anlamlı ilişkiler saptanmıştır. Ölçeğin Cronbach alfa iç tutarlık güvenirlik katsayısı içtenlik/samimiyet alt boyutu için α=.96, Kontrol alt boyutu için α=.85, düşmanlık/aşırı kızgınlık alt boyutu için α=.93 olarak hesaplanmıştır. Düzeltilmiş madde toplam korelasyonları .34 ile .92 arasında değişmektedir. Bu sonuçlara göre R-EDE’ nin Türk kültüründe ebeveynlik davranışlarını değerlendirmede kullanılabilecek geçerli ve güvenilir bir ölçme aracı olduğu söylenebilir.
Primary exploration of host–microorganism interaction and enteritis treatment with an embedded membrane microfluidic chip of the human intestinal–vascular microsystem
Authors: Zhao Wei , Yao Yuhan , Zhang Tong , Lu Huijun , Zhang Xinlian , Zhao Linlin , Chen Xi , Zhu Jinhui , Sui Guodong , Zhao Wang
Published: December 2022

Intestinal flora plays a crucial role in the host’s intestinal health. Imbalances in the intestinal flora, when accompanied by inflammation, affect the host’s intestinal barrier function. Understanding it requires studying how living cells and tissues work in the context of living organs, but it is difficult to form the three-dimensional microstructure intestinal–vascular system by monolayer cell or co-culture cell models, and animal models are costly and slow. The use of microfluidic-based organ chips is a fast, simple, and high-throughput method that not only solves the affinity problem of animal models but the lack of microstructure problem of monolayer cells. In this study, we designed an embedded membrane chip to generate an in vitro gut-on-a-chip model. Human umbilical vein endothelial cells and Caco-2 were cultured in the upper and lower layers of the culture chambers in the microfluidic chip, respectively. The human peripheral blood mononuclear cells were infused into the capillary side at a constant rate using an external pump to simulate the in vitro immune system and the shear stress of blood in vivo. The model exhibited intestine morphology and function after only 5 days of culture, which is significantly less than the 21 days required for static culture in the Transwell® chamber. Furthermore, it was observed that drug-resistant bacteria triggered barrier function impairment and inflammation, resulting in enteritis, whereas probiotics (Lactobacillus rhamnosus GG) improved only partially. The use of Amikacin for enteritis is effective, whereas other antibiotic therapies do not work, which are consistent with clinical test results. This model may be used to explore intestinal ecology, host and intestinal flora interactions, and medication assessment.
Adipose tissue-derived small extracellular vesicles modulate macrophages to improve the homing of adipocyte precursors and endothelial cells in adipose tissue regeneration
Authors: Dong Jia , Wu Bin , Tian Weidong
Published: December 2022

Rapid infiltration of endogenous cells induced by cell-free biomaterials is the first and crucial step in tissue regeneration and macrophage is largely involved. Our previous study reported adipose tissue-derived small extracellular vesicles (sEV-AT) could successfully promote adipose tissue regeneration. However, the role of macrophages in this process was unknown. In this study, we isolated sEV-AT and subcutaneously implanted it into the back of SD rats. The results showed sEV-AT increased macrophage infiltration significantly, which was followed by improving homing of adipocyte precursors (APs) and endothelial cells (ECs). However, when macrophages were depleted by clodronate liposome within 1 week, the homing of APs and ECs, and adipose tissue regeneration were destroyed. In vitro, sEV-AT showed the ability to promote the migration of macrophages directly. Besides, sEV-AT-pretreated macrophages improved the migration of APs and ECs, accompanied by the increase of chemokines (MCP-1, SDF-1, VEGF, and FGF) and the activation of NF-kB signaling pathway. These findings indicated sEV-AT might regulate the secretion of chemokines via activating NF-kB signaling pathway to improve homing of APs and ECs and facilitate adipose tissue regeneration. These findings deepened our understanding of small extracellular vesicle-induced tissue regeneration and laid a theoretical foundation for the clinical application of sEV-AT.
Role of casein kinase 1 in the amoeboid migration of B-cell leukemic and lymphoma cells: A quantitative live imaging in the confined environment
Authors: Čada Štěpán , Vondálová Blanářová Olga , Gömoryová Kristína , Mikulová Antónia , Bačovská Petra , Zezula Nikodém , Kumari Jadaun Alka , Janovská Pavlína , Plešingerová Hana , Bryja Vítězslav
Published: December 2022

The migratory properties of leukemic cells are commonly associated with their pathological potential and can significantly affect the disease progression. While the research in immunopathology mostly employed powerful indirect methods such as flow cytometry, these cells were rarely observed directly using live imaging microscopy. This is especially true for the malignant cells of the B-cell lineage, such as those originating from chronic lymphocytic leukemia (CLL) and mantle cell lymphoma (MCL). In this study, we employed open-source image analysis tools to automatically and quantitatively describe the amoeboid migration of four B-cell leukemic and lymphoma cell lines and primary CLL cells. To avoid the effect of the shear stress of the medium on these usually non-adherent cells, we have confined the cells using a modified under-agarose assay. Surprisingly, the behavior of tested cell lines differed substantially in terms of basal motility or response to chemokines and VCAM1 stimulation. Since casein kinase 1 (CK1) was reported as a regulator of B-cell migration and a promoter of CLL, we looked at the effects of CK1 inhibition in more detail. Migration analysis revealed that CK1 inhibition induced rapid negative effects on the migratory polarity of these cells, which was quantitatively and morphologically distinct from the effect of ROCK inhibition. We have set up an assay that visualizes endocytic vesicles in the uropod and facilitates morphological analysis. This assay hints that the effect of CK1 inhibition might be connected to defects in polarized intracellular transport. In summary, 1) we introduce and validate a pipeline for the imaging and quantitative assessment of the amoeboid migration of CLL/MCL cells, 2) we provide evidence that the assay is sensitive enough to mechanistically study migration defects identified by the transwell assay, and 3) we describe the polarity defects induced by inhibition or deletion of CK1ε.
In-silico molecular interactions among the secondary metabolites of Caulerpa spp. and colorectal cancer targets
Authors: Mert-Ozupek Nazli , Calibasi-Kocal Gizem , Olgun Nur , Basbinar Yasemin , Cavas Levent , Ellidokuz Hulya
Published: December 2022

Caulerpa spp. secrete more than thirty different bioactive chemicals which have already been used in cancer treatment research since they play a pivotal role in cancer metabolism. Colorectal cancer is one of the most common cancer types, thus using novel and effective chemicals for colorectal cancer treatment is crucial. In the cheminformatics pipeline of this study, ADME-Tox and drug-likeness tests were performed for filtering the secondary metabolites of Caulerpa spp. The ligands which were selected from the ADME test were used for in silico molecular docking studies against the enzymes of the oxidative branch of the pentose phosphate pathway (glucose-6-phosphate dehydrogenase and 6-phosphoglutarate dehydrogenase), which is of great importance for colorectal cancer, by using AutoDock Vina. Pharmacophore modeling was carried out to align the molecules. Molecular dynamic simulations were performed for each target to validate the molecular docking studies and binding free energies were calculated. According to the ADME test results, 13 different secondary metabolites were selected as potential ligands. Molecular docking studies revealed that vina scores of caulerpin and monomethyl caulerpinate for G6PDH were found as −10.6 kcal mol-1, −10.5 kcal mol-1, respectively. Also, the vina score of caulersin for 6PGD was found as −10.7 kcal mol-1. The highest and the lowest binding free energies were calculated for monomethyl caulerpinate and caulersin, respectively. This in silico study showed that caulerpin, monomethyl caulerpinate, and caulersin could be evaluated as promising marine phytochemicals against pentose phosphate pathway enzymes and further studies are recommended to investigate the detailed activity of these secondary metabolites on these targets.
From PEF to PBF: What difference does the longer alkyl chain make a computational spectroscopy study of poly(butylene 2,5-furandicarboxylate)
Authors: Nolasco Mariela M. , Rodrigues Leonor C. , Araújo Catarina F. , Coimbra Mariana M. , Ribeiro-Claro Paulo , Vaz Pedro D. , Rudić Svemir , Silvestre Armando J. D. , Bouyahya Chaima , Majdoub Mustapha , Sousa Andreia F.
Published: December 2022

This work explores the conformational preferences and the structure-property correlations of poly(butylene 2,5-furandicarboxylate) (PBF), a longer chain analogue of the most well-known biobased polyester from the furan family, poly(ethylene 2,5-furandicarboxylate) (PEF). A thorough computational spectroscopic study–including infrared, Raman and inelastic neutron scattering spectroscopy, combined with discrete and periodic density functional theory calculations–allowed the identification of dominant structural motifs in the amorphous and crystalline regions. Discrete calculations and vibrational spectroscopy of semi-crystalline and amorphous samples strongly support the predominance of gauche, trans, gauche conformations of the butylene glycol fragment in both the crystalline and amorphous domains. In what concerns the furandicarboxylate fragment, amorphous domains are dominated by syn,syn conformations, while in the crystalline domains the anti,anti forms prevail. A possible crystalline structure–built from these conformational preferences and including a network of C-H···O hydrogen bond contacts—was optimized using periodic density functional theory. This proposed crystal structure avoids the unrealistic structural features of the previously proposed X-ray structure, provides an excellent description of the inelastic neutron scattering spectrum of the semi-crystalline form, and allows the correlation between microscopic structure and macroscopic properties of the polymer.
Efficient catalytic degradation of methylene blue by a novel Fe3+-TiO2@CGS three-dimensional photoelectric system
Authors: Li Jian , Wang Yufei , Guo Fanhui , Chen Juan , Wang Jinxi , Fan Xiaoyong , Li Baoning , Verma Santosh Kumar , Wei Qingbo , Yan Long , Wu Jianjun
Published: December 2022

In this study, a novel three-dimensional photoelectric system was designed and constructed for the degradation of methylene blue (MB) via photocatalysis, electrocatalysis, and photoelectric catalysis. To this end, a Ti/RuO2-IrO2-SnO2-CeO2 electrode was prepared via a thermal oxidation coating method and used as a dimensionally-stable anode (DSA). The cathode was made of a titanium sheet with Fe3+-doped TiO2 loaded on coal gasification slag (CGS) (Fe3+-TiO2@CGS) as a photocatalyst. The factors affecting the degradation efficiency, such as the supporting electrolyte, current density, and initial pH were systematically investigated. The results revealed Fe3+-TiO2@CGS three-dimensional photoelectric system exhibiting efficient synergistic performance of photocatalysis and electrocatalysis with a synergistic factor of 1.11. Photo-generated holes (h+) were generated by light irradiation and direct anodic oxidation. Furthermore, hydroxyl radicals (HO·) radicals were induced via other pathways. Such active species showed highly-oxidizing abilities, beneficial to the degradation of methylene blue (MB). The representative Fe3+-TiO2@CGS three-dimensional photoelectric system showed super high degradation efficiency at pH 11 and current density of 18.76 mA cm−2. Using NaCl as a supporting electrolyte, the degradation yield reached 99.98% after 60 min of photoelectrical treatment. Overall, the novel Fe3+-TiO2@CGS three-dimensional photoelectrical system looks very promising for the highly efficient catalytic degradation of organic contaminants.
A systematic assessment of stress insomnia as the high-risk factor for cervical cancer and interplay of cervicovaginal microbiome
Authors: Rai Ravina , Nahar Manisha , Jat Deepali , Gupta Neelima , Mishra Siddhartha Kumar
Published: December 2022

Cervical cancer is a dreaded form of cancer in women, the fourth most common cancer, with around 0.3 million females suffering from this disease worldwide. Over the past several decades, global researches have focused on the mitigation of cervical lesions and cancers and have explored the impact of physiological and psychological stress and insomnia on cervical pathogenesis. Furthermore, disruption of the cervicovaginal microbiome profiles is identified as an added high-risk factor for the occurrence of cervical cancer. The physiological regulation of stress has an underlying mechanism controlled via hypothalamic pituitary adrenal (HPA) and sympatho-adrenal medullary (SAM) axes. Disruptions in these axes have been identified as the factors responsible for maintaining the homeostasis balance. Recent studies on microbiomes have offered novel ways to combat cervical cancer and cervix infection by exploring the interplay of the cervicovaginal microbiome. Moreover, the integration of various immune cells and microbiome diversity is known to act as an effective strategy to decipher the cervix biological activity. Cytokine profiling and the related immune competence, and physiological stress and insomnia impart to the regulatory networks underlying the mechanism which may be helpful in designing mitigation strategies. This review addressed the current progress in the research on cervical cancer, HPV infection, immune cell interaction, and physiological stress and insomnia with the cervicovaginal microbiome to decipher the disease occurrence and therapeutic management.