bluuebunny/crossref_metadata_2025 huggingface dataset:
Two-years antibody responses following SARS-CoV-2 infection in humans: A study protocol
Authors: Arguni Eggi , Dewi Fatwa Sari Tetra , Fachiroh Jajah , Paramita Dewi Kartikawati , Lestari Septi Kurnia , Wiratama Bayu Satria , Susilaningrum Annisa Ryan , Kharisma Bara , Meisyarah Yogi Hasna , Sari Merlinda Permata , Farahdilla Zakiya Ammalia , Siswanto Siswanto , Sjaugi Muhammad Farhan , Sasongko Teguh Haryo , Lazuardi Lutfan
Published: August 2022

The long-term antibody response to the novel SARS-CoV-2 in infected patients and their residential neighborhood remains unknown in Indonesia. This information will provide insights into the antibody kinetics over a relatively long period as well as transmission risk factors in the community. We aim to prospectively observe and determine the kinetics of the anti-SARS-CoV-2 antibody for 2 years after infection in relation to disease severity and to determine the risk and protective factors of SARS CoV-2 infections in the community. A cohort of RT-PCR confirmed SARS-CoV-2 patients (case) will be prospectively followed for 2 years and will be compared to a control population. The control group comprises SARS-CoV-2 non-infected people who live within a one-kilometer radius from the corresponding case (location matching). This study will recruit at least 165 patients and 495 controls. Demographics, community variables, behavioral characteristics, and relevant clinical data will be collected. Serum samples taken at various time points will be tested for IgM anti-Spike protein of SARS-CoV-2 and IgG anti-Spike RBD of SARS-CoV-2 by using Chemiluminescent Microparticle Immunoassay (CMIA) method. The Kaplan-Meier method will be used to calculate cumulative seroconversion rates, and their association with disease severity will be estimated by logistic regression. The risk and protective factors associated with the SARS-CoV-2 infection will be determined using conditional (matched) logistic regression and presented as an odds ratio and 95% confidence interval.
Hypothalamic transcriptome analysis reveals male-specific differences in molecular pathways related to oxidative phosphorylation between Iberian pig genotypes
Authors: Heras-Molina Ana , Núñez Yolanda , Benítez Rita , Pesántez-Pacheco José Luis , García-Contreras Consolación , Vázquez-Gómez Marta , Astiz Susana , Isabel Beatriz , González-Bulnes Antonio , Óvilo Cristina
Published: August 2022

The hypothalamus is implicated in controlling feeding and adiposity, besides many other physiological functions, and thus can be of great importance in explaining productive differences between lean and fatty pig breeds. The present study aimed to evaluate the hypothalamic transcriptome of pure Iberian (IBxIB) and Large White x Iberian crossbreds (IBxLW) at 60 days-old, produced in a single maternal environment. Results showed the implication of gender and genotype in the hypothalamic transcriptome, with 51 differentially expressed genes (DEGs) between genotypes and 10 DEGs between genders. Fourteen genotype by sex interactions were found, due to a higher genotype effect on transcriptome found in males. In fact, just 31 DEGs were identified when using only females but 158 using only males. A higher expression of genes related to mitochondrial activity in IBxIB male animals (ND3, ND4, ND5, UQCRC2 and ATP6) was found, which was related to a higher oxidative phosphorylation and greater reactive oxygen species and nitric oxide production. IBxLW male animals showed higher expression of SIRT3 regulator, also related to mitochondrial function. When females were analysed, such differences were not found, since only some differences in genes related to the tricarboxylic acid cycle. Thus, the results indicate a significant effect and interaction of the breed and the sex on the hypothalamic transcriptome at this early age.
Association of hypoxia inducible factor 1-Alpha gene polymorphisms with multiple disease risks: A comprehensive meta-analysis
Authors: Harun-Or-Roshid Md. , Ali Md. Borqat , Jesmin  , Mollah Md. Nurul Haque
Published: August 2022

HIF1A gene polymorphisms have been confirmed the association with cancer risk through the statistical meta-analysis based on single genetic association (SGA) studies. A good number SGA studies also investigated the association of HIF1A gene with several other diseases, but no researcher yet performed statistical meta-analysis to confirm this association more accurately. Therefore, in this paper, we performed a statistical meta-analysis to draw a consensus decision about the association of HIF1A gene polymorphisms with several diseases except cancers giving the weight on large sample size. This meta-analysis was performed based on 41 SGA study’s findings, where the polymorphisms rs11549465 (1772 C/T) and rs11549467 (1790 G/A) of HIF1A gene were analyzed based on 11544 and 7426 cases and 11494 and 7063 control samples, respectively. Our results showed that the 1772 C/T polymorphism is not significantly associated with overall disease risks. The 1790 G/A polymorphism was significantly associated with overall diseases under recessive model (AA vs. AG + GG), which indicates that the A allele is responsible for overall diseases though it is recessive. The subgroup analysis based on ethnicity showed the significant association of 1772 C/T polymorphism with overall disease for Caucasian population under the all genetic models, which indicates that the C allele controls overall diseases. The ethnicity subgroup showed the significant association of 1790 G/A polymorphism with overall disease for Asian population under the recessive model (AA vs. AG + GG), which indicates that the A allele is responsible for overall diseases. The subgroup analysis based on disease types showed that 1772 C/T is significantly associated with chronic obstructive pulmonary disease (COPD) under two genetic models (C vs. T and CC vs. CT + TT), skin disease under two genetic models (CC vs. TT and CC + CT vs. TT), and diabetic complications under three genetic models (C vs. T, CT vs. TT and CC + CT vs. TT), where C allele is high risk factor for skin disease and diabetic complications (since, ORs > 1), but low risk factor for COPD (since, ORs < 1). Also the 1790 G/A variant significantly associated with the subgroup of cardiovascular disease (CVD) under homozygote model, diabetic complications under allelic and homozygote models, and other disease under four genetic models, where the A is high risk factor for diabetic complications and low risk factor for CVD. Thus, this study provided more evidence that the HIF1A gene is significantly associated with COPD, CVD, skin disease and diabetic complications. These might be the severe comorbidities and risk factors for multiple cancers due to the effect of HIF1A gene and need further investigations accumulating large number of studies.
Thin-layer drying characteristics, modeling and quality attributes of tomato slices dried with infrared radiation heating
Authors: El-Mesery Hany S. , Reham M. Kamel  , Alshaer W. G.
Published: August 2022

An Infrared dryer was used to examine the drying of tomato slices. In this investigation, the influence of infrared radiation (IR) on the rate of drying, physical quality, energy combustion of tomato was estimated at three different levels of intensity at 0.15, 0.20, and 0.35 W/cm² under different air flows of 0.5, 1, and 1.5 m/s. Tomato slices were dried with an initial moisture content of 19.7 to 0.17 g water/g dry solids by infrared drying. The moisture content and drying rates are found to be dramatically affected by infrared density. An increase in the drying rate and a decrease in the drying period occurred with increasing infrared intensity. A decrease in energy consumption was detected with the increase of radiation intensity. The results clarified that the shrinkage ratio increased with increasing infrared intensity. The rehydration ratio raised with the increase in radiation intensity. The change in the colour difference of dried slices increased with an increase in radiation intensity. The models were in comparison using (R²) coefficient of determination, modelling efficiency (EF), and (χ²) reduced chi-square. Midilli model was fit for simulation of all drying conditions and could be used to estimate tomato moisture content at any time during the infrared drying process.
Propagation of yellow passion fruit seedlings by cutting, grafting and seeds under salt stress
Authors: Dos Santos Moura Regiana , Raj Gheyi Hans , Lima Cruz Amanda , Da Silva Nascimento Bruna , Pinto de Menezes Emanuela , Antônio Coelho Filho Mauricio
Published: August 2022

Salinity is one of the most limiting abiotic stresses in the global agricultural sector. The objective of this study was to evaluate the effect of irrigation water salinity on the initial development of P. edulis Sims. seedlings propagated by seeds, grafting and cuttings. Treatments were distributed in a completely randomized design, in a 5 x 3 factorial arrangement, corresponding to 5 levels of irrigation water electrical conductivity (0.3 - control, 1.8, 3.3, 4.8, and 6.3 dS m-1) and 3 propagation methods (seeds, cuttings and grafting), with four replicates and one plant per plot. Fifty days after the imposition of treatments with saline water, plant height, number of leaves, stem diameter, total chlorophyll, stomatal conductance, relative leaf water content, total water consumption, dry matter of root, shoot and total, root length and root volume were evaluated. The increase in water salinity affected with greater intensity the growth and development variables (height, total dry matter and root volume of the plant) and the physiological characteristics (stomatal conductance) of the species Passiflora edulis, regardless of the method of propagation. Seedlings propagated by grafting showed better development compared to the other propagation methods (seeds and cuttings). The interaction between the propagation methods and water salinity affected seedlings propagated by seeds and cuttings with greater intensity.
Evaluating the responses of some wheat landraces and cultivars cultivated locally in Saudi Arabia to three sources of irrigation water
Authors: Sultan F. Alsharari  , Ibrahim A. A. Almohisen
Published: August 2022

This study was aimed to investigate the response of some wheat (Triticum aestivum L.) landraces to three irrigation sources, freshwater (FW), well water (WW), and treated municipal wastewater (TMW), regarding the impact on growth, yield, and grains elements contents. The results showed that the various wheat landraces irrigated with treated wastewater were significantly taller (117.3 cm) with multi tillers (22 tiller plant-1), had maximum spikes per plant (2.6), and longer weighty spikes (14.5 cm & 12.1 g). Landraces L1(Burr), L2 (Baldy Burr), C7 (Yecora Rojo), had maximum tillers, L4 (Alssamaa Burr), L5 (Bahaal Burr), L7 (Yecora Rojo) had more spikes per plant and the longer weighty spikes were recorded in L5 Baldy Burr, L1(Burr), heavy spikes were reported in L5 (Bahaal Burr) and L1(Burr). Moreover, these landraces had the highest yield per plant and 1000 grains weight (49.8 g, 12.5 g) respectively. The N, P, K, and Mg contents were increased under TMW, and their levels in landraces and cultivars in order are 3>L2>L4>L5>L1>C6>C7. Even Cu, Fe, Mn, Zn levels were higher in various landraces irrigated with TMW, however, L3, L2, and L4 had maximum contents of all microelements. TMW irrigation enhanced growth, yield, and grain quality in terms of essential elements. The irrigation of landraces L1, L4, L5 with TMW may be a feasible alternative for sustainable wheat production and safe water in arid regions such as Saudi Arabia.
Extracts and purified substances of Cabralea canjerana inhibit hatching and extracts of Schinus terebinthifolius kill juveniles of meloidogyne incognita
Authors: Gomes Vanessa Alves , Carolina Alves Gomes  , Fabíola de Jesus Silva  , Márcio Santos  Soares  , Everaldo Antonio Lopes
Published: August 2022

Nematicidal substances have been identified from plants and are potentially useful for the management of plant-parasitic nematodes. Cabralea canjerana, (Meliaceae) and Schinus terebinthifolius (Anacardiaceae) produce bioactive compounds during their secondary metabolism and little is known about the effect of such substances on plant-parasitic nematodes. In the present study, we assessed the effect of aqueous and ethanolic extracts of C. canjerana and S. terebinthifolius at 1% (m:v) and purified substances from C. canjerana (gedunin, ocotillone, cabraleadiol, a mixture of ocotillone + cabraleadiol and a mixture of shoreic acid + eichlerianic acid) on hatching and mortality of Meloidogyne incognita juveniles. Aqueous extracts of C. canjerana fruits and seeds reduced hatching by 70.3 to 95.7%. Aqueous extracts of S. terebinthifolius fruits killed 42.8 to 77.1% of juveniles. The purified substances of C. canjerana inhibited the hatching of M. incognita from 57 to 90% and did not increase the mortality of juveniles. Therefore, C. canjerana extracts and its purified substances reduce M. incognita hatching and aqueous extracts of S. terebinthifolius kill J2 of this nematode.
Functional performance, nutritional status and level of physical activity of elderly
Authors: Ramos da Cunha Muniz Vivianne , Carvalho Fonseca Meneses Carla Cristina , Resende Gonçalves de Carvalho Cecília , Da Silva Santos Oliveira Amanda Suellenn , Rebelo Sampaio da Paz Suzana Maria , Figueiredo de Carvalho Marcos David , Carvalho Martins Maria do Perpétuo Socorro , Martins de Sousa Daniele , De Carvalho Cunha Sales Ana Lina , Guarino de Moura Sá Guilherme , Brito Lira de Carvalho Vanessa , De Morais Lima Geovanni , De Carvalho e Martins Maria do Carmo
Published: August 2022

The elderly is a risk group for changes in health conditions and as well as in functional performance related to the decline in grip strength and reduced walking speed, which can compromise the performance of activities of daily living. Based on this, the objective was to analyze the factors associated with functional performance in the elderly. For that, a cross-sectional study was developed involving a group of 179 elderly people who received medical care at an outpatient clinic of the public health network. Muscle strength and gait speed were measured. Nutritional status was determined by Body Mass Index (BMI), calf circumference (CC), waist circumference (WC) and waist-to-hip ratio (WHR). Level of physical activity was measured by the adapted version of the Minnesota Leisure Activities Questionnaire. Low muscle strength was found in 20.7% of the elderly and slow gait in 20.1%. Increased risk of cardiovascular disease was found in 40.8% considering WC, and 35.2% by WHR. Most of the elderly had low weight and no loss of muscle mass. Loss of muscle strength was associated with sex and CC and gait speed were associated with sex, age, and presence of a partner (p < 0.05). The chance of loss of muscle strength and lower gait speed was higher in elderly women. Loss of muscle mass due to CC was associated with the chance of loss of strength, and the chance of reduced walking speed was greater among the elderly individuals aged 75 years and over.
Indonesian basic olfactory terms: more negative types but more positive tokens
Authors: Siahaan Poppy
Published: August 2022

The present study investigates the semantics of a dozen basic smell terms in Indonesian using data from a large corpus of written register. Examining how these smell terms lexicalize some odors but not others raises questions that are central to our understanding of the language of olfaction. How are smell terms structured? What does the structure of smell terms tell us about human behavior? By applying cluster analysis, the present study reveals that the Indonesian odor lexicon is structured based on one dimension correlating with pleasantness. The large dataset of a written corpus enables the present study to reveal the differences in lexicalization and frequency: Indonesian smell terms have more negative types but more positive tokens in texts. This novel approach to investigating smell terms allows us to take a step closer toward our goal of understanding olfactory vocabulary, as data on token frequency are difficult to obtain in studies of (unwritten) minority languages. This key finding supports the Pollyanna Hypothesis: people tend to use positive words more often than negative words, but the negative words convey more information.
Synthesis of ring-A serjanic acid derivatives and their cytotoxic evaluation through the brine shrimp lethality assay (BSLA)
Authors: Galarraga Elier , Gómez Sherezade , Mavares Andersson , Urdaneta Neudo , Amaro-Luis Juan Manuel
Published: August 2022

Sixteen serjanic acid derivatives were synthesized by modification of the A-ring of the triterpenoid skeleta. Fischer indolization of intermediate 2 with the requisite aromatic hydrazines afforded the indolo-triterpenes 8–13. Also, reaction of 2 with hydroxylamine hydrochloride in pyridine provided the desired C-3 oxime 14, while pyrazine 15 was obtained by condensation of 2 in the presence of ethylenediamine and sulphur in morpholine. Finally, the Claisen-Schmidt condensation of intermediate 2 with corresponding substituted benzaldehydes 16–23, afforded benzylidine ketones 24–31. All compounds were elucidated on the basis of NMR and HR-MS spectroscopic data and evaluated for their in-vitro cytotoxicity to the Brine Shrimp Lethality Assay (BSLA). As a result, the compounds exhibited medium to good cytotoxic potential and this activity was as high as eight times that of serjanic acid (1) on the tested zoophytes.
Impact of continuous electrochemical degradation of eucalyptus alkaline peroxide mechanical pulping wastewater
Authors: Li Ming
Published: August 2022

Levels of pollutants in pulp and papermaking wastewater are an important issue for environmental protection. High concentrations of contaminants can seriously affect the environment if they are not processed. Therefore, treating paper wastewater is a necessary option for governments to select appropriate treatment methods to remove the multi-component pollutants. In this study, the impact of continuous electro-degradation of eucalyptus alkaline peroxide mechanical pulping wastewater was studied. An electrolytic treatment method performed at a low voltage was used. Through the electrolysis method, the wastewater chemical oxygen demand was decreased by 98%, and color-generating groups were nearly all destroyed. During the electrolysis process, the pH value of wastewater continuously decreased and finally reached 2.5. Lowering the pH of the waste solution will help increase the electrolysis efficiency of the wastewater. A total of 5.8 g of alkali was obtained. The wastewater precipitation before and after electrolysis was analyzed by X-ray photoelectron, Fourier transform infrared, and energy-dispersive X-ray spectroscopies.
On Sets of Points in General Position That Lie on a Cubic Curve in the Plane
Authors: Makhul Mehdi , Pinchasi Rom
Published: August 2022

Let P be a set of n points in general position in the plane. Let R be a set of points disjoint from P such that for every x, y € P the line through x and y contains a point in R. We show that if is contained in a cubic curve c in the plane, then P has a special property with respect to the natural group structure on c. That is, P is contained in a coset of a subgroup H of c of cardinality at most |R|.

We use the same approach to show a similar result in the case where each of B and G is a set of n points in general position in the plane and every line through a point in B and a point in G passes through a point in R. This provides a partial answer to a problem of Karasev.

The bound is best possible at least for part of our results. Our extremal constructions provide a counterexample to an old conjecture attributed to Jamison about point sets that determine few directions.
An UPLC-MS/MS method for quantification of spiraeoside in mouse blood and its application to a pharmacokinetic and bioavailability study
Authors: Cai Xiaojun , Dai Xiangyi , Li Zhou , Chen Junying , Wang Xianqin , Zhang Meiling
Published: August 2022

A simple, rapid, and sensitive method based on UPLC-MS/MS was developed to determine spiraeoside in mouse blood, and was applied to the pharmacokinetics and bioavailability of spiraeoside after mice after intravenous (a dose of 5 mg kg−1) and oral (a dose of 20 mg kg−1) administration. On HSS T3 column set at 40 °C, chromatographic separation was obtained with the mobile phase of acetonitrile and 0.1% formic acid using the gradient elution. Spiraeoside and internal standard (IS) were quantitatively analyzed using multiple reaction monitoring (MRM) mode in electrospray (ESI) positive interface. The MRM mode was monitoring the fragmentation of m/z 465.4→303.1 and m/z 451.3→ 289.2 for spironoside and IS, respectively. The results showed a good linear relationship was in the concentration range of 1–200 ng mL−1 (r > 0.998) and the lower limit of quantification (LLOQ) was 1.0 ng mL−1. The intra- and the inter-day precision (RSD%) of the method was within 14.0%, and the accuracy ranged from 90.0% to 115.0%. The extraction recovery of spriaeoside was better than 63.0%, and the matrix effects were in the range of 86%–98%. It also showed the half-life was short, and the absolute bioavailability was 4.0% in mice. Therefore, the established UPLC-MS/MS method was suitable for the pharmacokinetic and bioavailability study of spiraeoside in mice.
Isolation and molecular identification of obligate thermophiles from hot springs in Ba Ria – Vung Tau and Khanh Hoa provinces, Vietnam
Authors: Kha Mong Tran , Thanh Van Le Thi , Vu Phong Nguyen , Huu Tri Nguyen
Published: August 2022

Environments with temperatures from 50°C to 80°C are rare in nature and are almost exclusively associated with geothermal regions including hot springs, solar-heated soils and volcanic areas. Thermophilic bacteria already exist and prefer in such habitats. Since innate tolerance to thermal environment and potential chassis for extracellular enzymes such as lipase, protease and amylase, which are utilized widely in the industrial fermentation, thermophilic bacteria have been becoming one of the objects for microbiologists worldwide, recently. This study aimed to isolate and identify thermophilic bacteria from hot springs in several provinces in Vietnam such as Ba Ria - Vung Tau and Khanh Hoa. In the results, six moderate thermophilic bacterial strains (namely BM7, BS5, NS1, NS3, NS4, and NW6) that could grow at 55oC were purified from the hot spring ecosystems. All micro morphology of isolates were recorded as rod-shaped, Gram positive, and endospore forming. The results of 16S rDNA sequencing and phylogenetic analysis showed that these isolate belonged to group I of Bacillus genus (the thermophilic group). The isolated strains NS1, NS3, NS4, BS5, NW6 and BM7 were identified to belong to the Bacillus genus, species as Bacillus sp. Resulting strains are potential candidates for industrial applications due to its stable fitness in a hash environment, particularly at high temperature. In addition, this study provides a useful insight into the diverse community of thermophilic bacteria (Bacillus spp.) in several hot springs of Vietnam, that can be applied as bacterial cell factories to produce biomaterials, biofuels, or valuable compounds in the future.
Purification and characterization of recombinant nattokinase from Bacillus subtilis R0H1
Authors: Thuy Ngan Nguyen Thi , Tuan Le , Huong Nguyen Lan
Published: August 2022

Nattokinase (NK) is a fibrinolytic enzyme with the potential for fighting cardiovascular diseases (CVD) thanks to its antithrombotic, antihypertensive, anticoagulant, anti-atherosclerotic, and neuroprotective effects. Nattokinase was first discovered and purified from soybean fermented food by Bacillus subtilis natto. To enhance NK’s activity and simplify downstream processes, production of recombinant NK using several microbial expression systems such as Escherichia coli, B. subtilis, and Lactococcus lactic has been studied. Among all of them, B. subtilis is a prominent host for overproduction of functional proteins which can be secreted directly into the culture medium. In this study, recombinant NK from B. subtilis R0H1 was purified using two-step membrane filtration. Results showed 3.2-fold increase in activity and a recovery rate of more than 80%. Molecular weight of NK was approximately 28 kDa and its fibrinolytic degradation capacity was proved according to SDS-PAGE. The optimal pH and temperature of this NK were 8.5 and 55°C, respectively. The enzyme activity was boosted by Mg2+, Ca2+ and obviously inhibited by Co2+, Zn+2, Fe2+, and SDS. The apparent Km and Vmax with fibrin as the substrate were 3.08 mM and 6.7 nmol/min, respectively. The results suggested that membrane filtration is a useful method for purification of recombinant NK from B. subtilis R0H1. Therefore, application of membrane system is proposed to purify NK at the pilot scale. In addition, our findings indicated that recombinant NK produced in B. subtilis R0H1 showed high and stable proteolytic activity in slightly alkaline pH and at high temperature. It also exhibited strong fibrinolytic activity again both substrates: fibrinogen and fibrin.
Identification, cultivation condition of Parasevetibacter sp C101 and antimicrobial activity of the bacterial culture extract
Authors: Tuan Nguyen Manh , Quang Vien Le , Phuc Hung Truong , Minh Quan Tran , Hien Do Thi , Bich Due Do , Khuyen Duong Thi
Published: August 2022

During the recent decade, uncultured bacteria have been special interest as potential candidates for discovering novel antibacterial compounds. Two strains C101 and C102 were negative Gram bacteria, only growing on low nutrient media as R2A/3, NB/3, LB/10 and R4/10 compared to the usual. On R2A/3 medium, colonies of the isolates were round, convex, lemon yellow color with the size of 1-1.5 mm after six days of incubation at 28oC. Cells were 0.2-0.3 × 0.8-1.3 µm. The strains C101 and C102 were able to grow at temperature ranging 15-37oC (optimum at 25-28oC), pH 5-8 (optimum in pH 6-7). The sequences of 16S rRNA genes from strain C101 (MT756087) and C102 (MT756088) shared 100% identity. Analysis of full-length 16S rRNA gene sequence of strain C101 via using NCBI Blast, EzTaxon Database revealed the highest similarity of 99.18-100% to uncultured clones, and 97.86% to type species as Parasegetibacter terrae SGM2-10T. Genetic sequence analysis data showed that strain C101 should be considered a novel candidate species of the genus Parasegetibacter. Antibacterial compound was extracted from culture of strain C101 in R4/10 medium for ten days of shaking incubator at 28oC and exhibited susceptible activity to inhibit Bacillus anthracis KEMB 211-146 at a concentration of 2 µg/L and Staphylococcus aureus ATCC 6538 at 4 µg/L; intermediate inhibiting Bacillus subtilis KEMB 51201-001 at 8 µg/L, Staphylococcus epidermidis ATCC 14990 at 8 µg/L, and S. aureus CCARM 3155 at 16 µg/L; inhibition of S. aureus CCARM 3095 at 64 µg/L, S. aureus CCARM 3192 at 32 µg/L, and S. epidermidis CCARM 3710 at 64 µg/L.
Expression study of stress-related genes in salinity-treated transgenic Arabidopsis harboring soybean Response Regulator 34
Authors: Thai Huyen Pham Ngoc , Lan Xuan Hoang Thi , Nguyen Chuong Nguyen , Phuong Thao Nguyen
Published: August 2022

Owing to their sessile nature, plants are easily affected by various extenal factors. Among those, drought and salinity are considered as the most common stresses, which often pose a threat to plant growth and development. Major effects of the drought and salinity are interconnected and drive similar series of molecular changes in plants. These alterations in response to the stress are under the regulation of various signaling pathways, including the engangement of evolutionarily conserved two-component systems (TCSs). Three components with distinct functions can be found in a functional TCS, which are histidine kinases (HKs), histidine-containing phosphotransfer proteins (HPts), and response regulator proteins (RRs). Previous research revealed that the soybean (Glycine max) GmRR34 acts as an important regulatory protein in plants under drought stress conditions. In this project, the investigation on the role of GmRR34 in osmotic stress responses was extended to salinity by examining the expression of a subset of salinity-responsive genes using RT-qPCR method. Our analyses showed that the transgenic Arabidopsis plants ectopically expressing GmRR34 displayed enhanced expression of several important stress-related genes, including Catalase 1 (CAT1), Stromal ascorbate peroxidase 1 (sAPX1), Copper/zinc superoxide dismutase 1 (CSD1), Sodium/hydrogen exchanger 1 (NHX1) and Salt overly sensitive 2 (SOS2). These results indicate that GmRR34-transgenic plants might be more salt-tolerant thanks to stronger activities of antioxidant enzymes and better capacity in maintaining cytosolic ion homeostasis. Therefore, it is highlighted the necessity to perform further studies to fully characterize the GmRR34 biological functions as well as explore its application potential in enhancing the salt tolerance of crop plants.
In silico study of cytochrome P450 alleles and phenotypic distribution in Vietnamese population
Authors: Ngoc Ha Pham , Phan Tuan Nguyen , Thi Xuan Trinh , Nam Hai Truong , Dang Hung Tran , Cuong Nguyen
Published: August 2022

Cytochrome P450 enzymes play an important role in phase I drug metabolism, accounting for approximately 75% of the enzymatic processes. We investigated the allele and phenotypic distributions of five important CYP genes (CYP2B6, CYP3A5, CYP2C9, CYP2C19, CYP2D6) in the Vietnamese population by using Stargazer and the PharmCAT tool to call star alleles and translating them into phenotypes based on the available dataset of PharmGKB. We compared our computational analysis of the Vietnamese distributions with those of East Asia, Europe, America and other super populations, as well as with previous experimental research. The allele frequencies and phenotypic distributions of the five important CYP genes in the Vietnamese population are similar to those in East Asia while significantly different from other populations. In silico analysis also provided consistent results with previous experimental studies. In addition, the resultant data from our research contributes to the database of genetic variations in pharmacogenetics and constructs the fundamentals for future basic and applied research.
ARRANJO FAMILIAR, APOIO SOCIAL E FRAGILIDADE EM IDOSOS DA COMUNIDADE: ESTUDO LONGITUDINAL COM MÉTODOS MISTOS
Authors: Mota Gabriela Marques Pereira , Cesário Larissa Cayla , Jesus Isabela Thaís Machado de , Lorenzini Elisiane , Orlandi Fabiana de Souza , Zazzetta Marisa Silvana
Published: August 2022

RESUMO Objetivo analisar, ao longo do tempo, a constituição dos arranjos familiares de idosos e a relação destes com o apoio social, a fragilidade, qualidade de vida e cognição. Também, verificar, na perspectiva destes sujeitos, tensões existentes no contexto familiar. Método estudo longitudinal, com métodos mistos e triangulação concomitante. Aplicaram-se entrevista sociodemográfica, Escala de Fragilidade de Edmonton, WHOQOL-BREF e OLD, The Montreal Cognitive Assessment, Genograma e Ecomapa. Os dados quantitativos utilizaram teste de comparação de Wilcoxon e Mann Whitney; e os de abordagem qualitativa, a análise de conteúdo de Bardin, com o materialismo dialético como referencial teórico. Resultados dos 84 idosos no período do estudo (2012/2016-2019), a maioria tinha mais de 70 anos e era do sexo feminino (83,3%). A fragilidade e cognição não apresentaram relação estatística significativa com o tipo de arranjo familiar. Idosos que moravam sozinhos apresentaram pior qualidade de vida nos domínios físico (p=0,044) e psicológico (p=0,031). Idosos que moravam com netos apresentaram piora no domínio relação social (p=0,047) e melhora no domínio morte e morrer (p<0,001). Encontraram-se três categorias e nove subcategorias, as quais evidenciaram a importância das relações de interdependência e apoio na família. A integração dos dados demonstrou que o tamanho do arranjo familiar e os tipos de membros não determinam a existência de apoio, mas os vínculos formados com a família e comunidade. Conclusão a fragilidade e cognição não apresentaram diferença estatística com o tipo de arranjo familiar, porém essa relação foi encontrada em alguns domínios da qualidade de vida.
O que os personagens dizem os colocam na história: a crise argentina dos anos 2000 pelas falas de “Nueve Reinas” e “Familia Rodante”
Authors: Taño Debora Regina , Reck Miranda Suzana , Cesarino Costa Flávia
Published: August 2022

Características, ações e falas de personagens são elementos que exteriorizam o contexto de produção dos filmes. Obras como “Nueve Reinas” e “Familia Rodante” evidenciam por meio destes recursos comportamentos decorrentes de situações críticas, como a crise econômico-social argentina que teve seu ápice em 2001. A análise destas falas permite encontrar indícios que vão além do ambiente ficcional, alcançando seu contexto real.
A lembrança como corpo do tempo: diálogos sobre comunicação e temporalidades a partir de João Guimarães Rosa
Authors: Coração Cláudio , Vieira William David
Published: August 2022

Aqui, abordamos a demanda comunicacional de culto à velocidade/aceleração do tempo e seu confronto com a ideia de demora/contemplação. Exemplo desse embate entre pressa e vagar, o conto A Terceira Margem do Rio, de João Guimarães Rosa, mostra-se uma metodologia de reflexão. Por ele, acionamos a lembrança (um cerne do conto) como a esteira de uma temporalidade única (ou um rio que corre infinitamente), associamos outras figuras midiáticas e travamos diálogos sobre comunicação e temporalidades.
MEU TEMPO, MEU ESPAÇO? reflexões sobre temporalidades jornalísticas e no jornalismo
Authors: Prado Denise Figueiredo Barros do , Tavares Frederico de Mello Brandão , Tavares Michele da Silva
Published: August 2022

O espaço, mais do que um plano onde se inscrevem sistemas e objetos, é uma articulação material e imaterial, resultado da ação temporal dos sujeitos no mundo. Neste trabalho, propõe-se refletir sobre o binômio tempo/espaço, revelando, por meio de um estudo de caso, traços da relação jornalismo e sociedade. Tem-se como objeto de análise a edição #251, de fevereiro de 2016, da revista brasileira TRIP, um volume especial sobre o espaço e as formas de se habitá-lo. A partir da interpretação dos sentidos movimentados pela empiria, busca-se indicar formas de apreender as afetações existentes entre produção e representação, no âmbito de uma publicação, considerando como, reciprocamente, espacialidades e temporalidades indicam caminhos para a compreensão de historicidades editoriais.
Juventude, jornalismo e credibilidade em tempos de pandemia
Authors: Mendes Larissa de Morais Ribeiro , Felix Carla Baiense , Procópio Carla Ramalho , De Souza Luiza Gould , Fontes Helen Helen Pinto de Britto
Published: August 2022

Este artigo apresenta e analisa os resultados de pesquisa sobre o consumo jornalístico de jovens brasileiros entre 15 e 29 anos durante a pandemia de Covid-19. Os dados foram obtidos entre nov. e dez. de 2020, a partir de survey online, distribuído nas cinco regiões do Brasil. Entre os achados está o aumento do consumo de notícias e da confiança em veículos tradicionais, como a tevê. A informação pelo WhatsApp é muito consumida, mas tida como pouco crível.
Limits to the Jurisdiction of States in Private Law Matters under International Law
Authors: Noll Max
Published: August 2022

For decades, public and private international law scholars have debated whether the customary international law on the jurisdiction of States applies to private law. In this article, I argue that it does. Subsequently, I analyse the content of the customary law on jurisdiction of States in different fields of private law with an emphasis on tort law.
Investigation of Eccentrically-Supported Simple Beam under Point Load
Authors: TÜRKER Hakan T.
Published: August 2022

Klasik kiriş teorilerinde kirişler bir boyutlu kabul edilir. Bu teoriye göre mesnetler tarafsız eksendedir. Ancak pratik uygulamalarda kirişler tarafsız eksenlerinden farklı noktalardan mesnetlenmektedir. Literatürde üniform yük etkisinde eksantrik mesnetlenme durumuna sahip kirişler için düzenlenmiş kiriş teorisi geliştirilmiştir. Bu çalışmada tekil yük altındaki eksantrik mesnetli kirişler MacLaurin serileri kullanılarak analitik olarak çözülmüştür. Eksantrik mesnetlerin kirişin eğilme rijitliği üzerindeki etkileri araştırılmıştır. Elde edilen analitik denklemler, mesnetlerin kiriş derinliğinde farklı konumlarında (eksantrisite) kirişlerin eğilme analizi üzerindeki etkisini araştırmak için kullanılmıştır. Bulgular, kirişlerin eğilme rijitliğinin eksantrik mesnet durumundan önemli ölçüde etkilendiğini göstermektedir. Elde edilen sonuçlar Sonlu Eleman çözümleri ile karşılaştırılmıştır.
STUDI DESKRIPTIF PENCAPAIAN INDEK PENDIDIKAN DALAM KOMPOSIT IPM JAWA BARAT TAHUN 1993-2006
Authors: Nurulpaik Iik
Published: August 2022

Penelitian ini dimaksudkan untuk mengevaluasi kinerja pencapaian komposit IPM di JawaBarat pada periode 1993-2006. Tujuan penelitian ini: 1) Mendeskripsikan dan menganalisispencapaian IP sebagai salah satu komposit pembentuk IPM (AMH, RLS, ILS, IP,) di Jawa Baratpada periode tahun 1993-2006.Penelitian ini bersifat analisis ex-post facto evaluation. Metode yang dipergunakan adalahanalisis forcasting dengan menggunakan teknik proyeksi. Data yang dikaji merupakan datasekunder yang dipublikasikan secara resmi oleh BPS.Hasil penelitian bahwa selama periode 1993-2006: 1) RLS bertambah 1,25 tahun;pencapaiannya 7,80 tahun/tahun; pertumbuhannya 2,37% (0,18486/tahun). 2) AMHbertambah 6,96%; pencapaiannya sebesar 95,07%; pertumbuhannya 0,50% (0,475385%/tahun); buta hurup tersisa 4,27%. 3) AHH bertambah 7,23 tahun (68,28 tahun);pencapaiannya 67,70/tahun; pertumbuhannya 0,54% (0,36558 tahun). 4) IP bertambah7,47 poin; pencapaiannya 80,9 poin/tahun; pertumbuhannya 0,91%/tahun (0,73 poin).Selama periode 1993-2006 IP di Jawa Barat mencapai lompatan sebesar 7,47 poin. Rataratapencapaiannya 80,9 poin/tahun sedangkan pertumbuhannya mencapai 0,91%/tahun atau(0,73 poin). Pada tahun 2006 IP sudah mencapai 81,69 poin. Dengan kinerja tersebut padatahun 2010 IP diperkirakan akan mencapai 84,61 poin. Data menunjukan ternyata pencapaianIP tersebut lebih banyak disumbang oleh faktor AMH sebesar 79.05% sementara RLSmenyumbang 20,94%. Pertumbuhan selama kurun waktu lebih kurang 13 tahun terakhir initidak menggambarkan akselerasi yang progresif, artinya pula pembangunan pendidikan berjalanrelatif stagnan tanpa ada perubahan yang berarti. RLS hanya bertambah 1,25 tahun denganpertumbuhan 2,37% (0,18486 tahun) dan masing ada gap RLS 6,96 tahun. Dilihat dariakselerasi secara umum perkembangan angka RLS di semua daerah menunjukkan pertumbuhanpositif walaupun amat lamban dan cenderung stagnan, fluktuasinya antara 6-7 tahun.Secara keseluruhan akselerasi pencapaian RLS di daerah Kota lebih tinggi dibandingkandaerah Kabupaten. Fenomena ini sekaligus merefleksikan masih terjadinya ketimpangan antaradaerah perkotaan dan nonperkotaan sekaligus menunjukan pula bahwa perlakuan pembangunanmasih belum merata.
SURVEI PENELUSURAN PENGGUNA LULUSAN PROGRAM STUDI PENDIDIKAN MANAJEMEN PERKANTORAN
Authors: Sarino Alit , M.Pd Adman , TS Uep
Published: August 2022

Tujuan penelitian ini adalah untuk mengkaji Program studi Program Studi PendidikanManajemen Perkantoran sudah melakukan studi pelacakan kepada pihak pengguna lulusan dengan caramenyebarkan angket atau questioner dan penjaringan informasi melalui jejaring sosial (facebook) untukmengevaluasi kinerja lulusan oleh pihak pengguna lulusan. Penyebaran angket ini diberikan melaluilulusan yang sudah bekerja maupun melalui Ikatan Alumni Program Studi Pendidikan ManajemenPerkantoran.Tracer dilakukan oleh tim yang ditunjuk di bawah koordinasi ketua Program Studi PendidikanManajemen Perkantoran yang hasilnya didistribusikan ke kelompok bidang ilmu sebagai referensi dalammelakukan evaluasi dan penyempurnaan terhadap kurikulum. Untuk melakukan verifikasi terhadaphasil angket dilakukan kegiatan seminar dengan mengundang pengguna untuk dapat memberikanmasukan kepada program studi.Hasil diskusi ini merupakan salah satu dasar dalam melakukan review dan revise kurikulum.Metode penelitian yang digunakan adalah survey eksploratif. Sumber data adalah para pengguna lulusanprogram studi Pendidikan Manajemen Perkantoran. Pengumpulan data dilakukan melalui teknikwawancara, observasi, studi dokumentasi, dan penyebaran angket melalui media internet.Analisis data dilakukan dengan menggunakan presentasi relatif. Yaitu memperhitungkandan membandingkan dari satuan analisis dengan jumlah maksimal skore yang diperoleh. Selanjutnyavalidasi dilakukan melalui diskusi terbatas (focused group discussion), untuk memperoleh pemahamanyang bermakna dari konteks yang diteliti. Dengan demikian hasil penelitian dapat ditafsirkan secarakreatif, terbuka, dan bermakna bagi perbaikan mutu pendidikan.
Kualitas Investasi dalam Merintis dan Mengelola Usaha Kecil
Authors: Waspada Ika Putera
Published: August 2022

Kualitas Investasi dalam Merintis dan Mengelola Usaha Kecil
Front Matter Vol 3 No 1 Tahun 2004
Authors: Setiawan Yana
Published: August 2022

Front Matter Vol 3 No 1 Tahun 2004
Pemilihan Media, Visual, dan Pesan di Media Cetak, Elektronik dan Outdor Promotion
Authors: Kennedy John F
Published: August 2022

Pemilihan Media, Visual, dan Pesan di Media Cetak, Elektronik dan Outdor Promotion
Meningkatkan Kinerja Pelayanan Melalui Pemanfaatan Teknik Antrian
Authors: Sutarni Nani
Published: August 2022

Meningkatkan Kinerja Pelayanan Melalui Pemanfaatan Teknik Antrian
Pengaruh Budaya Organisasi Terhadap Kinerja Dosen di FPIPS Universitas Pendidikan Indonesia
Authors: Suwatno Suwatno
Published: August 2022

Pengaruh Budaya Organisasi Terhadap Kinerja Dosen di FPIPS Universitas Pendidikan Indonesia
Analaisis Implementasi Kebijakan Otonomi Pendidikan (Studi Terhadap Kebijakan Manajemen Berbasis Sekolah Sebagai Salahsatu Implementasi Kebijakan Otonomi Pendidikan di Jawa Barat)
Authors: Bestari Prayoga
Published: August 2022

Analaisis Implementasi Kebijakan Otonomi Pendidikan (Studi Terhadap Kebijakan Manajemen Berbasis Sekolah Sebagai Salahsatu Implementasi Kebijakan Otonomi Pendidikan di Jawa Barat)
Audit Komunikasi Sebagai Alat Mengukur efektivitas dan Efisiensi Komunikasi Organisasi
Authors: Suwatno Suwatno
Published: August 2022

Audit Komunikasi Sebagai Alat Mengukur efektivitas dan Efisiensi Komunikasi Organisasi
PENGARUH KOMPETENSI GURU TERHADAP KINERJA MENGAJAR GURU SMKN BIDANG KEAHLIAN BISNIS DAN MANAJEMEN DI KOTA BANDUNG
Authors: Sobandi Ade
Published: August 2022

Inti kajian penelitian ini adalah kinerja mengajar guru Sekolah Menengah KejuruanNegeri (SMKN) bidang keahlian Bisnis dan Manajemen di Kota Bandung. Banyak faktor yangmempengaruhi kinerja mengajar guru. Faktor yang mempengaruhi kinerja mengajar guru yangdikaji dalam penelitian ini adalah kompetensi guru. Berdasarkan hal tersebut, pokok masalahyang diungkap dalam penelitian ini adalah bagaimana pengaruh kompetensi guru terhadapkinerja mengajar guru. Penelitian ini merupakan tipe penelitian verifikatif dengan metodeyang digunakan adalah Explanatory Survey Method. Hasil penelitian menunjukkan bahwaKinerja mengajar guru SMKN bidang keahlian Bisnis dan Manajemen di kota Bandung beradapada kategori sangat baik. Kompetensi guru SMKN bidang keahlian Bisnis dan Manajemen dikota Bandung berada pada kategori baik. Hal ini menunjukkan kompetensi guru berpengaruhterhadap kinerja mengajar guru.
Unconditionally G ?odel Degeneracy for Quasi-Meager, Smooth Moduli
Authors: Singh Satpal
Published: August 2022

Let ? ? 0. A central problem in discrete Galois theory is the derivation of abelian, Hadamard, quasi-analytically Euler monodromies. We show that every number is naturally co-one-to-one, co-simply anti- Noetherian, pseudo-one-to-one and combinatorially unique. Recently, there has been much interest in the classification of pseudo-free isomorphisms. It is not yet known whether ? ? LD , although [13, 19, 28] does address the issue of connectedness.
Problems in Euclidean Probability
Authors: Alaria Satish Kumar
Published: August 2022

Let ? be a prime. We wish to extend the results of [31] to algebraic triangles. We show that there exists an algebraically Grassmann, count- able and globally ultra-bounded system. The groundbreaking work of D. Thomas on local, regular, hyper-globally arithmetic rings was a major ad- vance. Next, it has long been known that every element is super-minimal, stochastic, almost super-Selberg and Jacobi [25].
Mortality analysis in cases of COVID-19 during the second wave at a tertiary care center in western India
Authors: Pandey Rajesh , Mesharm Ashok
Published: August 2022

India witnessed a devastating second surge of COVID-19 cases from March 2021. Evidence strongly advocates its association with the patients’ age, gender, pre-existing comorbidity, vaccination status, and Remdesivir administration during the treatment of the disease. The objective of this study is to evaluate the significant relation of these five factors to in-hospital COVID-19 mortalities. We conducted a retrospective, cross-sectional, and observational cohort study between Jan 01 and May 30, 2021 in a tertiary care center in India. The outcome of interest is to identify the effect of vaccination, co-morbidities, and Remdesivir administration on COVID-19 mortality using SPSS software version 25.0 (IBM Corp., Armonk, NY, USA). The mortality rate was found to be 6.8 % (N=117) during hospitalization. The mean age of patients who died due to COVID-19 was 70.41 ± 15.04 years and the median was 68.34 (IQR: 59.61-83.38) years. About 89% of the population was over 55 years of age. The mortality rate was found to be higher in males (N=77; 65.8%). Out of 117 deaths, 95 patients (81.2%) were unvaccinated, whereas only 15.4% (N=18) and 3.4% (N=4) have taken a single and double dose respectively. Approximately, 3 quarter of patients had 1 or more comorbidity. Remdesivir administration is associated with the survival of 84.0% in moderate to severe COVID-19 infected patients. Results of our study coincide with the outcomes of studies done in the past concluding that age, gender, pre-existing comorbidities, vaccination status, and Remdesivir administration, these five factors are associated with COVID-19 mortalities.