bluuebunny/crossref_metadata_2025 huggingface dataset:
A Systematic Review of Spironolactone Nano-Formulations for Topical Treatment of Skin Hyperandrogenic Disorders and Chronic Wounds
Authors: Dereiah Saedah , Ghori Muhammad Usman , Conway Barbara R.
Published: December 2024

Background/Objectives: Spironolactone (SP), an aldosterone inhibitor widely used to treat androgen-dependent disorders such as acne, hirsutism, and alopecia, has demonstrated therapeutic potential in both oral and topical formulations. However, SP’s low solubility and poor bioavailability in conventional formulations have driven the development of novel nanocarriers to enhance its efficacy. This review systematically examines recent advancements in SP-loaded nanocarriers, including lipid nanoparticles (LNPs), vesicular nanoparticles (VNPs), polymeric nanoparticles (PNPs), and nanofibers (NFs). Methods: A search strategy was developed, and the relevant literature was systematically searched using databases such as Scopus, PubMed, and Google Scholar. The review process, including screening, inclusion, and exclusion criteria, adhered to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Results: A comprehensive analysis of 13 eligible research articles, corresponding to 15 studies, highlights key aspects such as encapsulation efficiency, stability, particle size, and in vitro and in vivo efficacy. Six studies focused on lipid nanoparticles (LNPs), including solid lipid nanoparticles (SLNs) and nanostructured lipid carriers (NLCs), which were found to improve SP’s bioavailability and skin permeation. Another six studies investigated vesicular nanoparticles (VNPs), such as ethosomes and niosomes, demonstrating superior skin targeting and penetration capabilities. Two studies on polymeric nanoparticles (PNPs) showed effectiveness in delivering SP to hair follicles for the treatment of alopecia and acne. Additionally, one study on SP-loaded nanofibers indicated significant potential for topical rosacea therapy. Conclusions: SP-loaded nanocarrier systems represent promising advancements in targeted topical therapy. However, further clinical studies are required to optimize their safety, efficacy, and delivery mechanisms.
Identification of Maize Kernel Varieties Using LF-NMR Combined with Image Data: An Explainable Approach Based on Machine Learning
Authors: Bi Chunguang , Bi Xinhua , Liu Jinjing , Chen He , Wang Mohan , Yu Helong , Song Shaozhong
Published: December 2024

The precise identification of maize kernel varieties is essential for germplasm resource management, genetic diversity conservation, and the optimization of agricultural production. To address the need for rapid and non-destructive variety identification, this study developed a novel interpretable machine learning approach that integrates low-field nuclear magnetic resonance (LF-NMR) with morphological image features through an optimized support vector machine (SVM) framework. First, LF-NMR signals were obtained from eleven maize kernel varieties, and ten key features were extracted from the transverse relaxation decay curves. Meanwhile, five image morphological features were selected using the recursive feature elimination (RFE) algorithm. Before modeling, principal component analysis (PCA) was used to determine the distribution features of the internal components for each maize variety. Subsequently, LF-NMR features and image morphological data were integrated to construct a classification model and the SVM hyperparameters were optimized using an improved differential evolution algorithm, achieving a final classification accuracy of 96.36%, which demonstrated strong robustness and precision. The model’s interpretability was further enhanced using Shapley values, which revealed the contributions of key features such as Max Signal and Signal at Max Curvature to classification decisions. This study provides an innovative technical solution for the efficient identification of maize varieties, supports the refined management of germplasm resources, and lays a foundation for genetic improvement and agricultural applications.
Mechanism of Action of Fusarium oxysporum CCS043 Utilizing Allelochemicals for Rhizosphere Colonization and Enhanced Infection Activity in Rehmannia glutinosa
Authors: Yuan Feiyue , Qiu Fuxiang , Xie Jiawei , Fan Yongxi , Zhang Bao , Zhang Tingting , Zhang Zhongyi , Gu Li , Li Mingjie
Published: December 2024

Rehmannia glutinosa is an important medicinal herb; but its long-term cultivation often leads to continuous cropping problems. The underlying cause can be attributed to the accumulation of and alterations in root exudates; which interact with soil-borne pathogens; particularly Fusarium oxysporum; triggering disease outbreaks that severely affect its yield and quality. It is therefore crucial to elucidate the mechanisms by which root exudates induce F. oxysporum CCS043 outbreaks. In this study; the genome of F. oxysporum CCS043 from R. glutinosa’s rhizosphere microbiota was sequenced and assembled de novo; resulting in a 47.67 Mb genome comprising 16,423 protein-coding genes. Evolutionary analysis suggests that different F. oxysporum strains may adapt to the host rhizosphere microecosystem by acquiring varying numbers of specific genes while maintaining a constant number of core genes.The allelopathic effects of ferulic acid; verbascoside; and catalpol on F. oxysporum CCS043 were examined at the physiological and transcriptomic levels. The application of ferulic acid was observed to primarily facilitate the proliferation and growth of F. oxysporum CCS043; whereas verbascoside notably enhanced the biosynthesis of infection-related enzymes such as pectinase and cellulase. Catalpol demonstrated a moderate level of allelopathic effects in comparison to the other two. Furthermore; 10 effectors were identified by combining the genomic data. Meanwhile; it was found that among the effector-protein-coding genes; ChiC; VRDA; csn; and chitinase exhibited upregulated expression across all treatments. The expression patterns of these key genes were validated using qRT-PCR. Transient overexpression of the two effector-encoding genes in detached R. glutinosa leaves provided further confirmation that ChiC (GME8876_g) and csn (GME9251_g) are key effector proteins responsible for the induction of hypersensitive reactions in R. glutinosa leaf cells. This study provides a preliminary indication that the use of allelochemicals by F. oxysporum CCS043 can promote its own growth and proliferation and enhance infection activity. This finding offers a solid theoretical basis and data support for elucidating the fundamental causes of fungal disease outbreaks in continuous cropping of R. glutinosa and for formulating effective mitigation strategies.
Phylogenetic Relationships in the Miracle Berry Genus, Synsepalum, Sensu Lato, and Relatives (Sapotaceae)
Authors: Potter Daniel , Uleh Mark
Published: December 2024

Synsepalum and Englerophytum are two closely related genera of the sub-family Chrysophylloideae in the family Sapotaceae. It has been reported that the two genera are a monophyletic group, and their generic limitations are uncertain. Synsepalum is an economically important genus that includes the medicinally and culinarily important plant, -miracle berry, S. dulcificum. The phylogenetic relationships among the species are poorly understood. This study has been conducted to refine the phylogenetic relationships between and within the two genera utilizing nuclear and chloroplast DNA data. Bayesian analyses and Maximum likelihood of nuclear internal transcribed spacer (ITS) and plastid (trnH-psbA) sequences were used to reconstruct the phylogeny of the two genera. Phylogenetic trees generated for both regions (nuclear and plastid) resulted in the resolution of six clades. Four of the clades correspond to species in the genus Synsepalum and two clades include species of Englerophytum. The two clades of Englerophytum are nested within Synsepalum suggesting that the two genera are closely related and may not merit their current circumscription as distinct genera. Also, Synsepalum is confirmed to constitute more than one lineage suggesting it is not monophyletic in its current definition. Overall, the study suggests the need for a comprehensive evaluation of all species currently recognized in the two genera.
Electrophysiological and Behavioral Responses of Orchestes steppensis (Coleoptera: Curculionidae) to Ulmus Plant Volatiles
Authors: Yang Meng , Li Qin , Zhao Guoshuai , Liu Yalin , Lou Yonggen
Published: December 2024

The flea-weevil Orchestes steppensis Korotyaev (Coleoptera: Curculionidae) is an Eastern Palaearctic Steppe species, and a serious pest of elm trees (Ulmus spp., Ulmaceae) by feeding on the leaves (adults) or mining them heavily (larvae) in Xinjiang, China. In order to search for chemical and ecological pest management practices, the olfactory preferences of O. steppensis for leaves of three elm species were investigated. The results revealed that O. steppensis has different host preferences for the three elm species: U. pumila L. first, followed by Ulmus laevis Pall. and Ulmus densa Litw. last. Volatile organic compounds from the leaves of the three Ulmus species were collected using dynamic headspace adsorption and analyzed through gas chromatography–mass spectrometry (GC-MS). A total of 94 volatile components in the healthy leaves and the infested leaves by O. steppensis of the three elm species were identified and analyzed, and 13 active compounds were identified using coupled gas chromatography–electroantennographic (GC-EAD) recording and GC-MS analysis. The response of O. steppensis to different concentrations of active compounds was determined using EAG, and the behavioral response to the highest EAG concentration of each active compound was determined. The results demonstrated that 3-hexen-1-ol, 3,7-dimethyl-1,3,6-Octatriene, methyl salicylate, 1-hexanol, and 3-hexen-1-ol, acetate were attractive to O. steppensis, while nonanal and 2-hexenal were repellent.
Mapping and Validation of Quantitative Trait Loci on Yield-Related Traits Using Bi-Parental Recombinant Inbred Lines and Reciprocal Single-Segment Substitution Lines in Rice (Oryza sativa L.)
Authors: Manzoor Ghulam Ali , Yin Changbin , Zhang Luyan , Wang Jiankang
Published: December 2024

Yield-related traits have higher heritability and lower genotype-by-environment interaction, making them more suitable for genetic studies in comparison with the yield per se. Different populations have been developed and employed in QTL mapping; however, the use of reciprocal SSSLs is limited. In this study, three kinds of bi-parental populations were used to investigate the stable and novel QTLs on six yield-related traits, i.e., plant height (PH), heading date (HD), thousand-grain weight (TGW), effective tiller number (ETN), number of spikelets per panicle (NSP), and seed set percentage (SS). Two parental lines, i.e., japonica Asominori and indica IR24, their recombinant inbred lines (RILs), and reciprocal single-segment substitution lines (SSSLs), i.e., AIS and IAS, were genotyped by SSR markers and phenotyped in four environments with two replications. Broad-sense heritability of the six traits ranged from 0.67 to 0.94, indicating their suitability for QTL mapping. In the RIL population, 18 stable QTLs were identified for the six traits, 4 for PH, 6 for HD, 5 for TGW, and 1 each for ETN, NSP, and SS. Eight of them were validated by the AIS and IAS populations. The results indicated that the allele from IR24 increased PH, and the alternative allele from Asominori reduced PH at qPH3-1. AIS18, AIS19, and AIS20 were identified to be the donor parents which can be used to increase PH in japonica rice; on the other hand, IAS14 and IAS15 can be used to reduce PH in indica rice. The allele from IR24 delayed HD, and the alternative allele reduced HD at qHD3-1. AIS14 and AIS15 were identified to be the donor parents which can be used to delay HD in japonica rice; IAS13 and IAS14 can be used to reduce HD in indica rice. Reciprocal SSSLs not only are the ideal genetic materials for QTL validation, but also provide the opportunity for fine mapping and gene cloning of the validated QTLs.
Elevated CO2 Shifts Photosynthetic Constraint from Stomatal to Biochemical Limitations During Induction in Populus tomentosa and Eucalyptus robusta
Authors: Tang Xianhui , Zhao Jie , Zhou Jiayu , Zhu Qingchen , Sheng Xiyang , Yue Chao
Published: December 2024

The relative impacts of biochemical and stomatal limitations on photosynthesis during photosynthetic induction have been well studied for diverse plants under ambient CO2 concentration (Ca). However, a knowledge gap remains regarding how the various photosynthetic components limit duction efficiency under elevated CO2. In this study, we experimentally investigated the influence of elevated CO2 (from 400 to 800 μmol mol–1) on photosynthetic induction dynamics and its associated limitation components in two broadleaved tree species, Populus tomentosa and Eucalyptus robusta. The results show that elevated CO2 increased the steady-state photosynthesis rate (A) and decreased stomatal conductance (gs) and the maximum carboxylation rate (Vcmax) in both species. While E. robusta exhibited a decrease in the linear electron transport rate (J) and the fraction of open reaction centers in photosynthesis II (qL), P. tomentosa showed a significant increase in non-photochemical quenching (NPQ). With respect to non-steady-state photosynthesis, elevated CO2 significantly reduced the induction time of A following a shift from low to high light intensity in both species. Time-integrated limitation analysis during induction revealed that elevated CO2 reduces the relative impacts of stomatal limitations in both species, consequently shifting the predominant limitation on induction efficiency from stomatal to biochemical components. Additionally, species-specific changes in qL and NPQ suggest that elevated CO2 may increase biochemical limitation by affecting energy allocation between carbon fixation and photoprotection. These findings suggest that, in a future CO2-rich atmosphere, plants productivity under fluctuating light may be primarily constrained by photochemical and non-photochemical quenching.
Sequential Defense Strategies: From Ant Recruitment to Leaf Toughness
Authors: dos Santos Danilo F. B. , Calixto Eduardo S. , Torezan-Silingardi Helena M. , Del-Claro Kleber
Published: December 2024

Plants express many types of defenses in response to herbivory damage. These defenses can be displayed as a sequence or they can overlap, increasing efficiency in protection. However, leaf defense shifts during leaf development, including extrafloral nectaries (EFNs), are neglected in natural tropical systems. To address this gap, our study evaluates the shifts in defense strategies of Eriotheca gracilipes, focusing on extrafloral nectaries and leaf toughness across leaf development stages. We also simulate herbivory by cutting the leaves to address the role of visiting ants against herbivores. We observed that E. gracilipes exhibits a defense turnover, shifting from indirect defenses (e.g., EFNs) in young leaves to physical defenses in adult leaves. Simulated herbivory led to heightened ant visitation, which correlated with decreased herbivory rates, indicating that ant recruitment acts as an effective deterrent. We observed a peak of EFN activity in young leaves, increased foliar toughness in adult leaves, and reduced herbivory on ant-patrolled young leaves. Additionally, E. gracilipes demonstrated tolerance to up to 10% foliar loss with no significant impact on leaf asymmetry, although 50% foliar loss increased asymmetry in newly flushed leaves. These results highlight E. gracilipes’ adaptive flexibility by attracting protective ants when vulnerable and enhancing structural defenses as leaves develops, E. gracilipes minimizes herbivory impact. This study provides valuable insight into the adaptive roles of EFNs and tolerance in E. gracilipes, contributing to a broader understanding of plant defense strategies.
Unveiling the Genetic Diversity and Demographic History of Coffea stenophylla in Sierra Leone Using Genotyping-By-Sequencing
Authors: Lahai Paul M. , Aikpokpodion Peter O. , Bah Alieu Mohamed , Lahai Mohamed T. , Meinhardt Lyndel W. , Lim Seunghyun , Ahn Ezekiel , Zhang Dapeng , Park Sunchung
Published: December 2024

Coffea stenophylla is a rare Coffea species boasting a flavor profile comparable to Arabica coffee (Coffea arabica) and has a good adaptability to lowland tropical climates. This species faces increasing threats from climate change, deforestation, and habitat fragmentation in its West African homeland. Using 1037 novel SNP markers derived from Genotyping-by-Sequencing (GBS), we revealed the presence of three distinct natural populations (mean Fst = 0.176) in Sierra Leone. Evidence of recent bottlenecks and small effective population size (118–140) was found across all three populations, reflecting the impact of recent anthropogenic disturbances on this species. Using a model-flexible inference approach, we unveiled a strong ancient bottleneck approximately 23,000 years ago, coinciding with the last glacial maximum (LGM), followed by post-glacial expansion and divergence into distinct genetic clusters. A comparative analysis between ex situ genebanks and natural populations detected a significant gap in genetic diversity, with two out of three natural populations missing from the ex situ genebank collection. These findings highlight the urgent need to improve conservation practices for C. stenophylla in Sierra Leone. The novel SNP markers developed in this study provided valuable tools to support future efforts in conservation and utilization of C. stenophylla genetic resources in West Africa.
A Proposed Saffron Soilless Cultivation System for a Quality Spice as Certified by Genetic Traceability
Authors: Mariani Alessandro , Marconi Gianpiero , Ferradini Nicoletta , Bocchini Marika , Lorenzetti Silvia , Chiorri Massimo , Russi Luigi , Albertini Emidio
Published: December 2024

Saffron (Crocus sativus L.) is one of the most expensive spices in the world due to its strong market demand combined with its labor-intensive production process, which needs a lot of labor and has significant costs. New cultivation methods and traceability systems are required to improve and valorize local Italian saffron production. In this study, we conducted a three-year trial in Umbria (Central Italy), looking for a soilless cultivation method based on wooden bins posted at a suitable height from the ground to ease the sowing of corms and harvesting of flowers. Moreover, the spice traceability could be based on investigating the genetic variability of Italian saffron populations using SNP markers. The proposed novel cultivation method showed significantly higher stigma and corm production than the traditional one. At the same time, the genetic analysis revealed a total of 55 thousand SNPs, 53 of which were specific to the Italian saffron populations suitable to start a food traceability and spice certification.
Comprehensive Analysis of β-1,3-Glucanase Genes in Wolfberry and Their Implications in Pollen Development
Authors: Zhang Xin , Zheng Pinjie , Wen Xurui , Bei Zhanlin
Published: December 2024

β-1,3-Glucanases (Glu) are key enzymes involved in plant defense and physiological processes through the hydrolysis of β-1,3-glucans. This study provides a comprehensive analysis of the β-1,3-glucanase gene family in wolfberry (Lycium barbarum), including their chromosomal distribution, evolutionary relationships, and expression profiles. A total of 58 Glu genes were identified, distributed across all 12 chromosomes. Evolutionary analysis revealed six distinct branches within wolfberry and nine distinct branches when compared with Arabidopsis thaliana. Expression analysis showed that 45 Glu genes were expressed in berries, with specific genes also being expressed in flowers and leaves. Notably, LbaGlu28 exhibited significant expression during the tetrad stage of pollen development and was localized in the cell wall. These findings provide valuable insights into the functional significance of Glu genes in wolfberry, highlighting their roles in development and their potential involvement in reproductive processes, particularly in pollen development.
Exogenous 2,4-Epibrassinolide Alleviates Alkaline Stress in Cucumber by Modulating Photosynthetic Performance
Authors: Nie Wenjing , He Qinghai , Ma Jinzhao , Guo Hongen , Shi Qinghua
Published: December 2024

Brassinosteroids (BRs) are recognized for their ability to enhance plant salt tolerance. While considerable research has focused on their effects under neutral salt conditions, the mechanisms through which BRs regulate photosynthesis under alkaline salt stress are less well understood. This study investigates these mechanisms, examining plant growth, photosynthetic electron transport, gas exchange parameters, Calvin cycle dynamics, and the expression of key antioxidant and Calvin cycle genes under alkaline stress conditions induced by NaHCO3. The findings indicate that NaHCO3 stress substantially impairs cucumber growth and photosynthesis, significantly reducing chlorophyll content, net photosynthetic rate (Pn), stomatal conductance (Gs), transpiration rate (E), maximum photochemical efficiency (Fv/Fm), actual photochemical efficiency (ΦPSII), antenna conversion efficiency (Fv′/Fm′), and photochemical quenching coefficient (qP). This disruption suggests a severe dysregulation of the photosynthetic electron transport system, impairing electron transfer from photosystem II (PSII) to photosystem I (PSI) and subsequently the Calvin cycle. Application of exogenous 24-epibrassinolide (EBR) alleviated these effects, reducing leaf chlorosis and growth inhibition and significantly enhancing the expression of key genes within the antioxidant system (AsA-GSH cycle) and the Calvin cycle. This intervention also led to a reduction in reactive oxygen species (ROS) accumulation and improved photosynthetic performance, as evidenced by enhancements in Pn, Gs, E, Fv/Fm, ΦPSII, Fv′/Fm′, and qP. Moreover, NaHCO3 stress hindered chlorophyll synthesis, primarily by blocking the conversion from porphobilinogen (PBG) to uroporphyrinogen III (UroIII) and by increasing chlorophyllase (Chlase) and decreasing porphobilinogen deaminase (PBGD) activity. Exogenous EBR countered these effects by enhancing PBGD activity and reducing Chlase activity, thereby increasing chlorophyll content under stress conditions. In summary, EBR markedly mitigated the adverse effects of alkaline stress on cucumber leaf photosynthesis by stabilizing the photosynthetic electron transport system, accelerating photosynthetic electron transport, and promoting the Calvin cycle. This study provides valuable insights into the regulatory roles of BRs in enhancing plant resilience to alkaline stress.
Magnetic Molecularly Imprinted Polymers with Hydrophilic Shells for the Selective Enrichment and Detection of Rosmarinic Acid in Aqueous Extraction
Authors: Wang Yanhui , Yan Linlin , Zheng Guangyao
Published: December 2024

Rosmarinic acid (RA) is a natural active compound widely found in many plants belonging to the family of Lamiaceae, Boraginaceae, and so on, which has various important bioactivities, including being anti-oxidative, anti-inflammatory, antiviral, etc. Herein, novel hydrophilic magnetic molecularly imprinted polymers (HMMIPs) with a regular core-shell structure were successfully developed using RA as a template molecule, acrylamide (AM) as a functional monomer, N-N ’methylenebisacrylamide (MBA) as a cross-linking agent, and water as the porogen. After a series of characterization and adsorption performance analyses, it was found that HMMIPs are hydrophilic with an adsorption capacity of 8.012 ± 0.54 mg/g, an imprinting factor of 3.64, and a selectivity coefficient of 2.63~2.91. Furthermore, the HMMIPs can be rapidly separated from other components under the influence of external magnetic fields. The HMMIPs were employed for the determination of RA present in the Perilla frutescens and Rosmarinus officinalis aqueous extract with recoveries of 88.2~107.3%. These results indicated that HMMIPs of RA have the benefits of straightforward operation, rapid adsorption, and high selectivity, rendering it an appropriate way for the expedient and selective isolation of RA in an intricate matrix.
Effects of Marquandomyces marquandii SGSF043 on the Germination Activity of Chinese Cabbage Seeds: Evidence from Phenotypic Indicators, Stress Resistance Indicators, Hormones and Functional Genes
Authors: Zheng Xu , Huang Yuxia , Lin Xinpeng , Chen Yuanlong , Fu Haiyan , Liu Chunguang , Chu Dong , Yang Fengshan
Published: December 2024

In this study, the effect of Metarhizium spp. M. marquandii on the seed germination of cabbage, a cruciferous crop, was investigated. The effects of this strain on the seed germination vigor, bud growth and physiological characteristics of Chinese cabbage were analyzed by a seed coating method. The results showed the following: (1) The coating agent M. marquandii SGSF043 could significantly improve the germination activity of Chinese cabbage seeds. (2) The strain concentration in the seed coating agent had different degrees of regulation on the antioxidase system of the buds, indicating that it could activate the antioxidant system and improve the antioxidant ability of the buds. (3) When the concentration of M. marquandii SGSF043 was 5.6 × 106 CFU/mL (average per grain), the effect of M. marquandii SGSF043 on the leaf hormones Indole Acetic Acid (IAA), Gibberellic Acid (GA) and Abscisic Acid (ABA) of Chinese cabbage seedlings was significantly higher than that of other treatment groups, indicating that the strain could optimize the level of plant hormones. (4) M. marquandii SGSF043 could induce the expression of stress-resistance-related genes in different tissue parts of Chinese cabbage and improve the growth-promoting stress resistance of buds. This study showed that M. marquandii SGSF043 could not only improve the germination vitality of Chinese cabbage seeds but also enhance the immunity of young buds. The results provide a theoretical basis for the application potential of Metarhizium marquandii in agricultural production.
Polymer Solutions in Microflows: Tracking and Control over Size Distribution
Authors: Bezrukov Artem , Galyametdinov Yuriy
Published: December 2024

Microfluidics provides cutting-edge technological advancements for the in-channel manipulation and analysis of dissolved macromolecular species. The intrinsic potential of microfluidic devices to control key characteristics of polymer macromolecules such as their size distribution requires unleashing its full capacity. This work proposes a combined approach to analyzing the microscale behavior of polymer solutions and modifying their properties. We utilized the idea of modeling cross-channel diffusion in polydisperse polymer microflows using dynamic light scattering size distribution curves as the source data. The model was implemented into a Matlab script which predicts changes in polymer size distribution at microfluidic chip outputs. We verified the modeling predictions in experiments with a series of microchips by detecting the optical responses of injected nematic liquid crystals in the presence of microfluidic polymer species and analyzing the polymer size distribution after microfluidic processing. The results offer new approaches to tuning the size and dispersity of macromolecules in solution, developing auxiliary tools for such techniques as dynamic light scattering, and labs-on-chips for the combined diagnostics and processing of polymers.
Application of Antimicrobial Rubber-Coated Cotton Gloves for Mangosteen-Peel-Extract-Mediated Biosynthesis of Ag–ZnO Nanocomposites
Authors: Luengchavanon Montri , Anancharoenwong Ekasit , Marthosa Sutida , Pengsakul Theerakamol , Szekely Jidapa
Published: December 2024

Nanocomposites based on metal nanoparticles (MNP) prepared with mangosteen (mgt) peel extract-mediated biosynthesis of Agmgt/Znmgt have attracted considerable interest due to their potential for various practical applications. In this study, their role in developing antibacterial protection for rubber cotton gloves is investigated. The process of mangosteen-peel-extract-mediated biosynthesis produced Agmgt/Znmgt nanocomposites with respective diameters of 23.84 ± 4.08 nm and 30.99 ± 5.73 nm, which were assessed in the context of antimicrobial rubber-coated gloves. The rubber glover surface exhibited a very dense deposition of the Ag+Znmgt nanocomposite, which subsequently demonstrated level 4 resistance to punctures under the ANSI-ISEA 105-2016 standard. This could be attributed to the Zn-cellulose double formation on the rubber surface. Notably, on testing the inhibition of bacterial growth, the extract with the Agmgt nanoparticles presented the least concentration capable of growth inhibition in comparison to the extracts with Znmgt and Ag+Znmgt nanoparticles. Each of the mangosteen extracts was shown to inhibit bacterial growth when tested against both Gram-positive cocci and Gram-negative bacilli, with MIC in the range 40–320 µg/mL. The growth of drug-resistant bacteria (MRSA) could also be inhibited with an MIC value of 160 µg/mL, and with 30 min of contact, gloves with respective coatings of Znmgt and Ag+Znmgt extract nanocomposites were shown to inhibit K. pneumoniae and MRSA. However, while effective bacterial inhibition occurred with the suspensions, the coatings on glove surfaces required a lengthy incubation period (contact time) of at least 30 min for efficacy.
Waste-Derived High-Density Polyethylene-Glass Composites: A Pathway to Sustainable Structural Materials
Authors: Wimalasuriya Lasan , Gunasekara Chamila , Robert Dilan , Setunge Sujeeva , O’Donnell Brian
Published: December 2024

Millions of tonnes of plastic and glass waste are generated worldwide, with only a marginal amount fed back into recycling with the majority ending at landfills and stockpiles. Excessive waste production calls for additional recycling pathways. The technology being investigated in this study is based on recycled glass fines encapsulated in a high-density polyethylene (HDPE) matrix. Laboratory tests are performed on specimens at different manufacturing conditions using compression moulding, determining an optimised manufacturing method. The performance of composites prepared under different formulations is tested to identify an optimised mix design by means of statistical analysis. At this optimum ratio, flexural, tensile, and compressive strengths of 33.3 MPa, 19.6 MPa, and 12.8 MPa, are, respectively, recorded. Upon identifying the optimum dosage levels, the potential for employing HDPE from diverse origins are investigated. The microstructure, pore structure, and chemistry of optimised composite specimens are analysed to interpret the composite performance. The effective stress transfer in the composite is attributed to strong hydrogen bonds created by maleic anhydride leading to 37.6% and 8.5% improvements in compressive and flexural strengths, respectively. These research findings can facilitate the pathway for utilising plastic and glass waste in landfills/stockpiles for sustainable polymeric composites towards structural applications.
Control of Dielectric, Mechanical, and Thermal Properties of a Polymer Composite Based on ABS Using CoB Nanoparticles
Authors: Khannanov Artur , Burmatova Anastasia , Balkaev Dinar , Rossova Anastasia , Zimin Konstantin , Kiiamov Airat , Cherosov Mikhail , Lounev Ivan , Kutyreva Marianna
Published: December 2024

This article is devoted to the development of a new method for the synthesis of magnetic cobalt boride nanoparticles using a low-energy approach. The obtained nanoparticles were used to create composite materials based on industrial thermoplastic ABS. The effect of different concentrations of nanoparticles on the physical, mechanical, magnetic, and dielectric properties of composite materials was studied. It was proven that by varying the concentration of nanoparticles in the ABS composite, it is possible to control the glass transition temperature from 107.5 to 112 °C. The resulting composites demonstrated superparamagnetic behavior, which changed linearly. The permittivity of the composite remained close to that of pure ABS, but a shift in the maximum permittivity to the low-frequency region was observed with an increase in the content of nanoparticles. Thus, a method for controlling the mechanical, magnetic, and dielectric properties of a composite material has been developed, which makes it possible to use routine ABS in a wider range of applications, including electrical devices.
Quantitative Analysis and Modeling of Transient Cuttings Transport Impact on Drill String Mechanics in Extended Reach Drilling
Authors: Xiang Jianbo , Wang Xi , Lou Wenqiang , Wang Xueying , Zhao Chi , Zhang Feifei
Published: December 2024

Cuttings beds in horizontal wells significantly affect the frictional torque and drag along the drill string; however, their quantification and modeling have been relatively underexplored. To gain deeper insights into the impact mechanisms of the cuttings bed distribution on drilling mechanics, this study establishes a model linking the cuttings bed height with variations in axial and tangential forces on the drill string through experimental investigations. By integrating this model with previously developed transient cuttings transport and torque–drag models, a coupled transient hole cleaning and drill string mechanics model is constructed. This comprehensive model simulates the dynamic distribution of cuttings along the entire well trajectory and its influence on the drill string torque and drag. The results reveal that accumulated cuttings significantly reduce the weight on bit (WOB), increase the drill string torque, and cause problems related to a high equivalent circulation density (ECD). For long horizontal sections, the key to achieving effective hole cleaning lies in optimizing the design of the tripping circulation time to ensure that all cuttings are removed from the wellbore. Using the proposed coupled model, a methodology is developed to minimize the tripping circulation time by solving optimization problems within a constrained 2D domain, providing scientific guidance for drilling operations. The findings demonstrate that dynamically managing the cuttings distribution in the wellbore can significantly mitigate issues arising from insufficient hole cleaning, thereby ensuring drilling safety and efficiency. This study provides a scientific foundation for the optimized design of long horizontal well drilling operations and highlights the critical role of cuttings management in enhancing hole cleaning performance and mitigating drilling risks.
Bubble Dynamics in Sustainable Technologies: A Review of Growth, Collapse, and Heat Transfer
Authors: Rashid Farhan Lafta , Al-Obaidi Mudhar A. , Hussein Ahmed Kadhim , Ahmad Shabbir , Albdeiri Mahmood Shaker , Mujtaba Iqbal M.
Published: December 2024

The study of bubble growth and collapse is of great significance in the context of sustainability due to its influence on numerous energy-related processes and technologies. Understanding the dynamics of bubble behavior is vital for optimising heat transfer efficiency, which has an energetic role in improving the performance of sustainable systems such as nuclear reactors, thermal inkjet printing, and nucleate boiling. Indeed, researchers can progress strategies to enhance the efficiency of these technologies by analysing the parameters influencing bubble growth and collapse, which can lead to reduced energy consumption and environmental impact. Although several theoretical models and experimental investigations have been achieved in the past to inspect bubble growth and collapse, a thorough review and critical assessment of the studies conducted have not yet been achieved. This review aims to provide a comprehensive understanding of the relationship between bubble dynamics and sustainability, highlighting the potential for further research and development in this area. Specifically, the scope and limitations of past research on bubble growth and collapse is conducted to fill this gap in the open literature. The review covers both numerical and experimental studies of bubble growth and collapse in a wide set of innovative industrial applications including nuclear reactors, thermal inkjet printing, nucleate boiling, hydrodynamic erosion, and ultrasonic and medicinal therapy. The current review also attempts to illustrate and evaluate the numerical methods used and underlines the most relevant results from the studies that were looked at in order to provide researchers with a clear picture of the growth and collapse of bubbles in different applications. The results give a precise understanding of the dynamics of bubble growth and collapse and the related temperature change and cumulative heat transmission from the thermal boundary layer. Additionally, it has been demonstrated that simulation-based models can effectively predict transport coefficients. However, the review observes a number of limitations of the past research on bubble growth and collapse. Due to numerical instability, very little work with respect to dynamic modelling has been carried out on the mechanisms of bubble collapse. Accordingly, a number of recommendations are made for the improvement of heat transmission during bubble growth and collapse. Specifically, future criteria for the highest heat transmission will demand more precise experimental and numerical approaches.
Wellhead Stability During Development Process of Hydrate Reservoir in the Northern South China Sea: Evolution and Mechanism
Authors: Li Qingchao , Li Qiang , Wu Jingjuan , Li Xianzhong , Li Hongbin , Cheng Yuanfang
Published: December 2024

Natural gas hydrates represent a promising clean energy source with vast reserves. Their efficient development is crucial for ensuring the sustainable advancement of human society. However, wellhead instability occurred in the long-term development, which poses a significant challenge that impacts its commercial development. In the present work, the properties of hydrate-bearing sediments were experimentally investigated. It was found that the elastic modulus, cohesion, and internal friction angle of hydrate-bearing sediments exhibit an increase with the effective stress. As an example, when the effective stress increases from 0 MPa to 25 MPa, the normalized elastic modulus exhibits a rise from 1.00 to 1.36. Conversely, the Poisson’s ratio, permeability, and porosity demonstrate a decline in accordance with this trend. As an example, both normalized porosity and permeability decrease to values below 0.40 as the effective stress increases to 25 MPa. Based on the experimental results and previous work, a comprehensive model for describing the effect of both hydrate saturation and effective stress on physical parameters was obtained. Subsequently, a multi-field coupled investigation methodology was developed to evaluate wellhead stability during the long-term development of hydrate-bearing sediments, and the evolution characteristics and mechanisms of wellhead instability were numerically explored. It reveals that development operation using the vertical wellbore decomposes hydrates in the surrounding sediments only within a radius of 19.52 m, which significantly undermines the wellhead stability. Moreover, the wellhead system not only sinks with sediment subsidence but also experiences additional sinking due to the failure of bonding between the wellhead system and sediments. Furthermore, the latter accounts for a significant portion, amounting to approximately 68.15% of the total sinking under the research conditions. This study can provide methodological prerequisites for exploring the impact of various factors on wellhead stability during the long-term hydrate development process.
Intraoral Immature Malignant Teratoma with No Evidence of Other Sites of Involvement in a 6-Year-Old Patient: A Case Report
Authors: Zisis Vasileios , Charisi Christina , Poulopoulos Konstantinos , Papadopoulos Petros , Poulopoulos Athanasios
Published: December 2024

Background and Clinical Significance: Head and neck teratomas are embryonal tumors that develop when totipotent germ cells escape the developmental control of primary organizers and form a more-or-less organoid mass in which tissues from all three germ layers (ectoderm, endoderm, and mesoderm) can be identified. Mature teratomas may either transit into germ cell or non-germ cell malignancies or remain histologically mature with the possibility of growing, thus inducing certain complications when reaching a large size. This article aims to investigate a very rare case of a 6-year-old child who exhibited a recurrent intraoral mass with multiple conflicting biopsies. Case Presentation: A 6-year-old male patient was referred to the postgraduate clinic of the Department of Oral Medicine/Pathology, Dental School, Faculty of Health Sciences, Aristotle University of Thessaloniki, Greece, because his pediatric dentist noticed an exophytic, intraoral mass, distal to tooth #75 during a routine checkup. The first histopathological examination showed a gingival tumor, classified as a small round blue cell tumor, with greater similarity to adamantinoma-like Ewing sarcoma (ALES) and less to synovial sarcoma. The second pathologist examined the same tissue specimen and suggested the extremely rare presence of an immature malignant teratoma. Following chemotherapy, the rest of the teratoma with the adjacent tooth #75 was removed, and the histopathological examination showed a mature teratoma. Conclusions: This case illustrates the crucial role of the dentist, and in this case of the pediatric dentist, to promptly diagnose the underlying disease. Genetic screening may assist in detecting high-risk populations. In such complex histopathological cases, the importance of cooperating with experienced oral and maxillofacial pathologists is highlighted. We describe a rare case of intraoral malignant teratoma, and an extended literature review revealed that our case is the first ever reported.
Impact of Hop Residue Reuse on the Chemical and Sensory Properties of Craft Beer
Authors: Mejia-Llontop Cesar I. , Tirado-Rodríguez Carlos E. , Acosta-Baca Alanis , Aguayo-Flores Maylee , Ascate-Pasos Manuel , Ayala-Jara Carmen , Rodriguez Gilbert , Villanueva Eudes , Aguirre Elza
Published: December 2024

Hops are an important component of beer brewing, providing aromatic and bittering properties that are essential to consumer appeal. A significant amount of hop residue is generated in the dry-hop brewing process that cannot be reused due to bittering residues that disqualify them as animal feed or other products. The purpose of this research was to reuse four varieties of hop waste (Citra, Mosaic, Hallertau Blanc, and Mandarina Bavaria) through a repalletization process with the objective of integrating them into a new craft beer brewing process. Chemical properties such as the phenolic content, antioxidant capacity, and α- and β-acids were significantly reduced (p < 0.05) due to the reuse of the repelletized hops, leading to a decrease in the bitterness levels in all of the craft beers brewed with dry-hop residues. Finally, the sensory study conducted with non-habitual craft beer consumers revealed significant general acceptability for beers brewed with repelletized dry-hop residues (Mandarina Bavaria, Citra, and Mosaic). The reuse of hop residues for brewing presents a promising opportunity for further development in the food industry.
Inverse and Forward Kinematics and CAD-Based Simulation of a 5-DOF Delta-Type Parallel Robot with Actuation Redundancy
Authors: Laryushkin Pavel , Antonov Anton , Fomin Alexey , Fomina Oxana
Published: December 2024

This article introduces a novel modification of a Delta-type parallel robot. The robot has five degrees of freedom and provides its end-effector with a 3T2R motion pattern (three translational and two rotational degrees of freedom). The fifth degree of freedom (rotation) is kinematically decoupled from the other four motions, and it is controlled by two drives. Thus, the proposed robot has a redundant actuation. In this article, we present an algorithm to solve the inverse kinematics of this robot and apply it to a path modeling example of a spiral-like trajectory. Numerical simulations illustrate the algorithm and show how the actuated coordinates change along the considered trajectory. Forward kinematics follows next, and an approach is introduced to determine all end-effector configurations for the specified displacements in the actuated joints. A numerical example presents four assembly modes of the robot corresponding to four real solutions of the forward kinematic problem. Finally, this article demonstrates a computer-aided design and analysis of the proposed robot: we describe a procedure for analyzing inverse kinematics and calculating actuation torques. This study forms the basis for the future manufacturing and experimental analysis of a robot prototype.
Gridless DOA Estimation with Extended Array Aperture in Automotive Radar Applications
Authors: Jiang Pengyu , Gao Silin , Zhao Jie , Zhang Zhe , Zhang Bingchen
Published: December 2024

Millimeter-wave automotive radar has become an essential tool for autonomous driving, providing reliable sensing capabilities under various environmental conditions. To reduce hardware size and cost, sparse arrays are widely employed in automotive radar systems. Additionally, because the targets detected by automotive radar typically exhibit sparsity, compressed sensing-based algorithms have been utilized for sparse array reconstruction, achieving superior performance. However, traditional compressed sensing algorithms generally assume that targets are located on a finite set of grid points and perform sparse reconstruction based on predefined grids. When targets are off-grid, significant off-grid errors can occur. To address this issue, we propose an automotive radar sparse reconstruction algorithm based on accelerated Atomic Norm Minimization (ANM). By using the Iterative Vandermonde Decomposition and Shrinkage Threshold (IVDST) algorithm, we can achieve fast ANM, which effectively mitigates off-grid errors while reducing reconstruction complexity. Furthermore, we adopt a Generalized Likelihood Ratio Test (GLRT) detector to eliminate noise and clutter in the automotive radar operating environment. Simulation results show that our proposed algorithm significantly improves reconstruction accuracy compared to the iterative soft threshold (IST) algorithm while maintaining the same computational complexity. The effectiveness of the proposed algorithm in practical applications is further validated through real-world data experiments, demonstrating its superior capability in clutter elimination.