bluuebunny/crossref_metadata_2025 huggingface dataset:
A study of the level of circulating cytokines in patients with atopic dermatitis
Authors: Volkova YE. N. , Morozov S. G. , Tarasova M. V. , Grigoriyeva A. A. , Yelistratova I. V.
Published: February 2022

Goal of the study. To study the role of pro-inflammatory and anti-inflammatory cytokines for the course of atopic dermatitis (AD). Materials and methods. The study involved 72 patients aged 25-55 suffering from atopic dermatitis for 2-30 years and with at least two relapses a year. The AD patients were assigned to the following groups depending on the SCORAD (iSc) index: Group 1: light form (iSc < 30 points), Group 2: moderate to severe form (iSc from 30 to 50 points) and Group 3: severe form (iSc > 50 points). The control group comprised 23 practically healthy subjects aged 25-35. The level of cytokines (IL-1 β, -4, -6, -8, -10, -12, -13, TNF-α and IFN-γ) in blood serum was determined based on the immune-enzyme assay method. Results. A trend to the increased production of anti-inflammatory cytokines (IL-4, -6, -10, -13) at the exacerbation stage and increased synthesis of pro-inflammatory ones (IFN-γ, TNF-α and IL-12) at the remission stage was observed in patients with atopic dermatitis, which confirms the dysregulation of reciprocal relationships between Th1 and Th2 lymphocyte subpopulations during the disease.
Rationale for selecting antihistamine drugs for the therapy of chronic urticaria in terms of efficacy and safety
Authors: Skorokhodkina O. V. , Klyucharyova S. V.
Published: February 2022

Goal of the study. To compare the efficacy of antihistamine drugs for the therapy of chronic urticaria taking into consideration their effect on the patients’ cognitive functions. Materials and methods. The study involved 178 patients with chronic urticaria who were divided into six groups taking second generation antihistamine drugs: Cetirizine (n = 38), Levocetirizine (n = 27), Fexofenadine (n=26), Ebastine (n = 33), Loratadine (n = 26) and Desloratadine (n = 28). The patients recorded dynamic changes in clinical symptoms of the disease (number of urticarial components, skin itching intensity, availability or absence of urticarial derniographism, angioedema signs and signs of the shortness of breath and reduced blood pressure) in their individual diaries. Baseline signs of the patients’ cognitive condition and those recorded during the treatment were studied using the Kraepelin’s arithmetic test (modified by Schulte), I.M. Lushchikhina’s verbal and visual thinking assessment method and method for memorizing ten words. The control group comprised 31 subjects without chronic urticaria. Results of the study. Ebastine and Fexofenadine are the most efficient antihistamine drugs for the treatment of chronic urticaria. At the same time, they do not have any negative effect on the patients’ cognitive functions so they can be recommended for long-term treatment of chronic urticaria. In spite of its evident positive therapeutic effect, Cetirizine reduces mental alertness and deteriorates thinking in patients with chronic urticaria. Because of this, the drug must be prescribed with care for long-term administration to those patients whose professional activities demand increased attention concentration. Loratadine has a positive effect on the patients’ attention and thinking. However, taking into consideration its low efficacy, the drug can be prescribed as the basis therapy for the treatment of light forms of chronic urticaria.
Application of Safocid in clinical practice (review of the results of studies carried out in Russia)
Authors: Khryanin A. A. , Reshetnikov O. V.
Published: February 2022

This systematic review presents the data on using the Safocid complex drug for the treatment of a combination or unknown pathology of the urogenital tract as well as for the prevention of pre- and post-surgical complications in case of urologic and gynecologic interventions. According to the review of 39 articles, the authors of most of the publications assess the drug efficacy as high while the amount of adverse effects is minimum.
Centennial anniversary of the Saratov research association of dermatologists and venereologists
Authors: Zavyalov A. I. , Uts S. R. , Orkin V. F. , Morrison A. V.
Published: February 2022

The article describes major historic stages of the Saratov Research Association of Dermatologists and venereologists. The authors describe the Society’s role in the establishment and development of the dermatovenerological service, health education, prevention and improvement of measures aimed at fighting sexually transmitted and contagious skin diseases as well as training highly skilled personnel and promoting health care services in the region.
Actinic reticuloid: сase study
Authors: Tlish M. M. , Katkhanova O. A. , Naatij J. Y. , Popovskay E. B. , Sicheva N. L.
Published: February 2022

A rare case study of actinic reticuloid is presented. Analysis of literature, clinical and pathomorphological criteria of I diagnostics are described.
Clinical and morphological features of elastolytic granulomas
Authors: Patrushev A. V. , Khairutdinov V. R. , Belousova I. E. , Samtsov A. V.
Published: February 2022

The authors discuss current data on the classification, etiology and pathogenesis, clinical picture, pathologic features and treatment options of elastolytic granulomas (EGs). The authors disclose main aspects of elastolysis and elastophagocytosis development. Three cases of diseases from the EG group - actinic granuloma and annular elastolytic giant cell granuloma -are described for the first time in the Russian literature.
Topical methylprednisolone aceponate and dexpanthenol in the treatment of patients with atopic dermatitis
Authors: Chikin V. V.
Published: February 2022

The goals of the topical treatment of patients suffering from atopic dermatitis are suppression of the inflammatory reaction in the skin, elimination of itching, and recovery of the damaged protective lipid barrier of the epidermis. In this connection, topical glucocorticosteroids and skin moisturizers are used in the therapy of patients suffering from atopic dermatitis. Methylprednisolone aceponate (MPA, Advantan) being a topical glucocorticosteroid is characterized by high efficacy and safety. MPA is marked by the fast development of the therapeutic effect. This drug can be used for children older than 4 months and can be applied once a day. The availability of four dosage forms of MPA (emulsion, cream, ointment and fatty ointment) enables physicians to select a therapy with the use of this drug depending on the stage and localization of the skin lesion. Dexpanthenol (Bepanthen) being a tissue repair stimulator moisturizes the skin, protects the skin against an irritant effect, and restores the protective lipid barrier of the epidermis. The use of a combination of MPA being a topical glucocorticosteroid and dexpanthenol being a tissue repair stimulator can enhance the efficacy of the treatment of patients suffering from atopic dermatitis.
Foxp3+ T-lymphocytes in the pathogenesis of mycosis fungoides
Authors: Zhukov A. S. , Belousova I. E. , Samtsov A. V.
Published: February 2022

The pathogenesis of mycosis fungoides (MF) is not fully understood, nevertheless it is believed that the microenvironment of the tumor cells could influence the development of malignant lymphocytes. T-regulatory cells are a specialized subpopulation of T-lymphocytes, which can have an immunosuppressive effect and thus affect the immune response. Goal. The study population of regulatory T-cells in the skin of patients with mycosis fungoides and llaque parapsoriasis. Materials and methods. The content of Foxp3+ and CD3+ cells by immunohistochemical studies of skin biopsy specimens from patients with patch stage mycosis fungoides (10 patients) and plaque parapsoriasis (10 patients). The control group consisted of 16 healthy individuals. Results. Relative expression area Foxp3+ cells in patients with mycosis fungoides patch stage (0.44%) 2.6 times greater than in patients with plaque parapsoriasis (0,14%; p < 0,05). Part the Foxp3+ cells of the total number of T lymphocytes in patients with patch stage mycosis fungoides (5.9%) and plaque parapsoriasis (4.7%) were not significantly different (p > 0,05). Conclusion. In the initial stage of patients with mycosis fungoides determined a statistically significant predominance of the absolute number of T-regulatory cells in the epidermis and in the dermis. Part T-regulatory cells from all of T-lymphocytes in patients with skin patch stage mycosis fungoides and plaque parapsoriasis not statistically significantly different.
Non-invasive diagnostics of scarring alopecias by the method of trichoscopy
Authors: Kondrachine I. N. , Mareeva A. N.
Published: February 2022

Literary and own data on trichoskopy opportunities are presented with differential-diagnostic tools of non-cicatricial alopecia. Clinical data are discussed, the importance of a trichoskopy is established at diagnosis of patients with alopecia of different etiology.
Pathogenic aspects of treatment of psoriatic patients
Authors: Chikin V. V. , Znamenskaya L. F. , Mineyeva A. A.
Published: February 2022

The article describes high incidence and morbidity rate of psoriasis, substantial reduction in the life quality and psychosocial disadaptation of patients, and presents certain particular features of psoriasis pathogenesis taking into consideration the role of immune mechanisms and relation between the disease and other chronic processes in the organism, as a result of which psoriasis is considered to be a multimorbid condition. The multimorbidity of psoriasis is an important factor for selecting a therapy, especially for patients with severe forms of the disease.
Clinical manifestations of the skin photodamage as a result of a multi-course phototherapy of psoriasis patients
Authors: Zhilova M. B. , Volnukhin V. A. , Dvornikov A. S.
Published: February 2022

Goal. To study clinical manifestations and frequency of skin photodamage symptoms in psoriasis patients receiving a longterm multi-course phototherapy. Materials and methods. The study involved 106 patients suffering from psoriasis vulgaris and receiving a multi-course treatment by methods of the PUVA therapy, broadband medium-wave UV therapy and narrowband phototherapy with the wavelength of 311 nm. The average number of courses was 7 while the average number of treatment sessions was 141. Depending on the number of treatment sessions, the patients were divided into three groups: Group 1 (n = 33) - patients that received 50-100 phototherapy sessions, Group 2 (n = 58) - patients that received 101-200 treatment sessions, and Group 3 (n = 15) - patients that received over 200 treatment sessions. The control group comprised 20 psoriasis patients that did not receive any phototherapy before. Major results. The comparative analysis of the entire group of patients receiving a phototherapy revealed a statistically significant incidence of lentigo/sunspots, diffuse skin hyperpigmentation and actinic elastosis vs. the control group. The frequency of clinical manifestations characteristic of the skin photodamage grew as the number of treatment sessions increased. All of the symptoms except for guttate hypomelanosis and venous lakes demonstrated statistically significant dynamics. Conclusion. There is a dose-dependant increase in the frequency of skin photodamage symptoms in patients suffering from psoriasis vulgaris and receiving a long-term multi-course phototherapy.
Application of exifine in etiotropic treatment of zooanthroponotic trichophytia
Authors: Hismatullina Z. R. , Danilenko R. U. , Sultanbaeva A. U. , Mukhamadeeva O. R.
Published: February 2022

We have studied effect of Exifine on the dynamics of infectious process and some parts of the immune response at different stages of zooanthroponotic trichophytosis development. Functional activity of neutrophils, CD3, CD4, CD8, CD16, CD72, HLA-DR lymphocyte markers, TNFa, IL-1 β, IL-4 and IFN-γ cytokines were determined using immunological studies. Histomorphological changes of affected hair were studied during treatment. Effectiveness of Exifine at zooanthroponotic trichophytosis of scalp and smooth skin in children has been proven: mycological cure and persistent therapeutic effect. Exifine has no immunosuppressive activity as observed in Griseofulvin treatment.
Endothelial dysfunction and its treatment in psoriasis patients
Authors: Shtoda Yu. M. , Slesarenko N. A. , Bakulev A. L. , Utz S. R. , Platonova A. N.
Published: February 2022

Goal. To assess the effect of a combined cytostatic therapy and sulodexide on the functional condition of the vascular endothelium in psoriatic patients. Materials and methods. The authors examined 74 patients suffering from psoriasis vulgaris and exudative form of psoriasis. Depending on the treatment option, the patients were subdivided into two groups: Group I (n = 41) receiving a cytostatic therapy and Group II where patients received sulodexide as well. The severity of the endothelium dysfunction was assessed based on laboratory assessments such as nitrogen oxide and endothelin 1 in the blood serum. Results. Prior to the treatment, the level of nitrogen oxide was reduced and endothelin 1 was increased in the blood serum of most patients. Substantial improvement of the pathological process was observed in patients from Group I after the cytostatic therapy (total dose of methotrexate: 100 mg); no normalization of laboratory assessments was observed. In patients from Group II receiving methotrexate (total dose: 40 mg) along with sulodexide, improvement or recovery was observed after the completion of the treatment; endothelium dysfunction was normalized. Conclusion. The inclusion of sulodexide in a complex therapy normalizes endothelial dysfunction in case of psoriasis and reduces the dose and duration of methotrexate treatment.
Optical coherence tomography and polarimetry of superficial skin biopsies
Authors: Utz S. R. , Zimnyakov D. A. , Galkina E. M. , Reshetnikova E. M.
Published: February 2022

The goal. Of this study was to develop and assess the efficacy of polarization probing of biotissues in vitro. The method is based on the determination of polarization parameters of scattered radiation. Materials and methods. The well-known superficial epidermis stripping method was applied using the Sulfacrylate self-sterile medical adhesive. Small portions of thin layers of the adhesive were applied to slide plates and then to different skin sites. The corneous layer in the normal condition and in case of skin diseases (psoriasis, lichen acuminatus, discoid lupus erythematosus, alopecia, itching and demodectic mange) was examined based on the optical coherence tomography (OCT) method using the 0CS1300SS device (Thorlabs Inc, USA). Results. The authors obtained pictures visualizing the structural organization of different layers of the epidermis using the superficial epidermis biopsy method in case of lichen acuminatus, hyperkeratosis, itching and other skin diseases. Conclusion. This method ensures non-invasive high-precision measurement of the structure of different layers of the epidermis, which may be useful both for research purposes and practical dermatology.
Current concept of the diagnostics and treatment of vulvovaginal candidosis
Authors: Malbakhova YE. T. , Arzumanyan V. G.
Published: February 2022

In spite of high prevalence of vulvovaginal candidosis (VVC), its diagnostics and treatment pose a serious problem. Factors such as accessibility of antimycotic drugs, their uncontrolled application by patients and absence of any appropriate laboratory diagnostics result in the growth of the incidence rate, in particular, of recurrent forms related to a steady growth in the number of fungi of clinical importance as well as strains resistant to antifungal drugs. Discussion and conclusion. Today the problem of the need to develop VVC prevention drugs is increasingly urgent because reduced immunity results in relapses of the disease. Clinical trials of drugs for immune system correction are ongoing, and some of them have reached Phase 2. Based on the present-day research data, it is possible to conclude that the key standards for VVC diagnostics and treatment are as follows: mandatory diagnostics with the use of microbiological tests to determine the sensitivity to antifungal medicines, in particular, in case of recurrent VVC, and collection of detailed medical history data and physical examination to determine the form and severity of the disease (acute, recurrent, complicated or uncomplicated) and treatment duration: 1-7 days for acute uncomplicated forms and 7-14 days for recurrent complicated forms. The specific drug and route of administration are to be selected based on the physician’s preferences, patient’s convenience and cost of the drug provided the drug sensitivity is determined by laboratory tests. The administration of probiotics for VVC prevention and treatment is disputable today.
Vaginal candidal infection: clinical features of diagnostics methods
Authors: Rakhmatuuna M. R. , Gushchin A. YE. , Tsoi YE. G.
Published: February 2022

Goal. To study clinical features of the vaginal candidal infection and carry out a comparative analysis of the microscopy, cultural and real-time PCR methods for identifying Candida fungi. Materials and methods. The study involved 107 female subjects: Group 1: 56 women with Candida fungi found in their vaginal secrets by laboratory tests; Group 2: 51 women with absent clinical and laboratory signs of the urogenital infection. The laboratory test methods were as follows: microscopy, cultural and biomolecular (real-time PCR (RT PCR) - Florocenosis-Candida). Key findings. Pathologic vaginal secretion (76.8%) and hyperemia of vaginal and vulvar mucous tunic (41.1%) belong to the key clinical manifestations of the vaginal candidal infection. Caseous vaginal discharge was revealed in 33.9% of patients from Group 1, homogenous creamy discharge - in 42.9%, and homogenous mucous non-transparent vaginal discharge - in 23.2% of patients from Group 1. The patients had the following etiologic agents: C. albicans (94.6%), C. glabrata, (3.6%) and C. krusei (1.8%). Candida spp. identification results using the cultural and microscopy methods coincided in 78.6% of all cases, RT PCR and microscopy - in 66.1%, and RT PCR and cultural methods - in 87.5% of all cases. There were no reliable differences in the quantitative results for Candida fungi by the cultural and RT PCR methods: below 102 CFU/mL and below 102 GE/mL - 12.2 and 8.9%, respectively, 103-104 CFU/mL and 103-104 GE/mL - 44.9% and 33.9%, over 104 CFU/mL and over 104 GE/mL - 42.9 and 57.2%, respectively(p > 0.05). Conclusion. Candidal colonization of the vaginal mucous tunic was accompanied by specific clinical manifestations of urogenital candidosis in 76.8% of all cases; C. albicans was revealed in most of the subjects (94.6%). The real-time PCR method used for Candida spp. identification and quantitative determination demonstrated higher sensitivity and specificity vs. microscopy as well as sensitivity and specificity being comparable to the cultural diagnostics method.
Current features of the clinical course of urogenital candidosis and analysis of antifungal sensitivity of Candida fungi
Authors: Malova I. O. , Kuznetsova YU. A.
Published: February 2022

Based on the results of a study and treatment of 100 female patients suffering from chronic recurrent vulvovaginal candidosis (CRVC) and 50 patients suffering from concomitant CRVC and candidal intestinal dysbiosis (CID), the authors analyzed particular features of clinical manifestations of the disease, species composition and sensitivity of Candida fungi cultures sampled from vagina and bowels, and discuss the results of the treatment suggested based on the aforesaid study.
The cutaneous manifistation of Lyme Borreliosis: primary borrelial lymphocytoma and surrounding secondary erythema chronicum migrans
Authors: Sokolovskiy E. V. , Gorislavskaia T. A. , Mikheev G. N.
Published: February 2022

The authors describe a rare clinical observation of the cutaneous manifistation of Lyme Borreliosis: primary borrelial lymphocytoma and surrounding secondary erythema chronicum migrans. The review of clinical features, histology, is also present.
Case studies of skin melanoma
Authors: Kozlova A. V. , Shumbasov M. A. , Rybnikov YU. A. , Goldberg S. M. , Mikitin O. S. , Kazakov V. M. , Semenov E. V. , Ruksha T. G.
Published: February 2022

Skin melanoma is a malignant tumor originating in the cells of the melanocytic system, which is characterized by an aggressive clinical course, significant metastatic potential and unfavorable prognosis. These features of the tumor stipulate the need to improve measures to optimize early diagnosis of tumors. The article presents cases of pigmented skin melanoma to demonstrate the variability of clinical manifestations of this tumor requiring dermatologist skills in the differential diagnostics of neoplasms.
Medical cell technologies for treatment of patients suffering from recessive dystrophic epidermolysis bullosa. Method of intracutaneous administration of fibroblasts
Authors: Albanova V. I. , Karamova A. E. , Chikin V. V. , Mineyeva A. A.
Published: February 2022

Recessive dystrophic epidermolysis bullosa (RDEB) is a severe inherited disease developing due to genetic abnormalities in the synthesis of Type VII collagen by fibroblasts. A low production rate of Type VII collagen and abnormalities related to the formation of anchoring fibrils weaken the epidermis and derma adhesion strength, which results in the formation of blisters or erosions in case of any mechanical injury. Fibroblasts and keratinocytes belong to the key sources of Type VII collagen in the skin. Application of allogeneic fibroblasts is a promising cell technique for treating RDEB patients. The therapeutic effect of fibroblasts intradermal administration is stipulated by high stability of newly synthesized Type VII collagen and its ability to form anchoring fibrils in the area of the dermoepidermal junction. According to experimental and clinical studies, it is possible to boost the content of Type VII collagen in the dermoepidermal junction area and heal long-term skin defects in RDEB patients by means of intradermal administration of allogeneic fibroblasts.
Complex non-invasive methods of investigation of the pathophysiological processes in the skin of patients with rosacea
Authors: Kubanova A. A. , Makhakova YU. B. , Krupatkin A. I.
Published: February 2022

Goal. To develop a complex method for diagnosing rosacea by using non-invasive examination methods and study their application for assessing the treatment efficacy. Materials and methods. The authors conducted a study of 46 rosacea patients (15 male and 31 female) including 29 patients with erythematous and 17 ones with papulous subtypes of the disease. The patients underwent treatment using IPL (5-8 treatment sessions). The condition of skin vessels was assessed prior to and after treatment using dermatoscopy, laser Doppler flowmetry (LDF) and confocal laser scanning microscopy in vivo (CLSM). Results. The authors developed a complex method for diagnosing rosacea using dermatoscopy, CLSM and LDF techniques prior to and after treatment. As many as 46 patients suffering from erythematous and papulous subtypes of rosacea underwent treatment using broadband pulse light emission. The clinical efficacy of such treatment was assessed by using a set of non-invasive examination methods, and terms for new treatment courses were forecasted. Conclusion. A combination of different enables real-time assessment of the dynamics of the pathological process in the skin of patients suffering from rosacea and makes it possible to get unbiased information about the treatment efficacy and forecast terms for new treatment courses.
Пимекролимус в лечении атопического дерматита у детей. Вопросы безопасности и эффективности. Опыт пятилетнего применения
Authors: Zaslavsky D. V.
Published: February 2022

A growth in the incidence rate for atopic dermatitis (AD) in infants has been observed for this decade. Early formation of AD (at the age of 3-6 months) is observed in 45% of infants, and in 60% of infants - during the first year of life. As many as 65% of children (by the age of 7) and 74% of children (by the age of 16) suffering from AD have a spontaneous remission of the disease. As many as 20-43% of children suffering from AD further develop bronchial asthma, and the incidence rate of allergic rhinitis is twice as high. In spite of the fact that topical corticosteroids (TCS) are prescribed frequently enough, there is a need in the non-hormonal therapy due to poor compliance with the TCS treatment regimen in connection with side effects. Treatment of AD is often complicated by the colonization with Staphylococcus aureus and inefficacy of the external therapy with topical corticosteroids because many patients with AD have a high level of IgE against the superantigen of Staphylococcus. 1% pimecrolimus cream (PIM) and TCS were compared in a long-term large-scale study involving younger children suffering from mild to moderate AD. Materials and methods. The five-year open-label study involved 2,418 children, who were randomized into groups receiving PIM (n = 1205; in case of an aggravation - short-term administration of TCS) or TCS (n = 1213). The main goal of the study was to compare the safety of these two methods of treatment; an auxiliary goal was to confirm the long-term efficacy of PIM. Treatment was considered to be successful if the score based on the IGA scale was 0 (clean skin) or 1 (almost clean). Results. The effect in both groups of the drugs - PIM and TCS - was fast, and the success of treatment was recorded for >50% of patients by Week 3. In both groups, treatment was determined to be successful after 5 years in >85% of patients, and treatment of manifestations of AD on the face was efficient in 95% of all subjects. In the PIM group, the need in steroids was considerably lower than in the TCS group (7 days of administration vs. 178 days in the TCS group). In both groups, there were adverse events similar by their nature and frequency, and no disorders of humoral or cellular immunity were revealed. Conclusions. The long-term administration of PIM in case of mild to moderate AD in children was revealed to be safe and had no effect on the immune system. When PIM was used, the need in corticosteroids was considerably reduced (steroid-preserving effect). These data confirm that PIM is as efficient as TCS and can be used as the first-line therapy for mild to moderate AD in infants and younger children.
Treatment of rosacea patients with broadband pulse light emission using smooth pulse and photon recirculation technologies
Authors: Kubanova A. A. , Makhakova Y. B.
Published: February 2022

Goal of the study. To assess the efficacy and safety of using broadband pulse light emission with smooth pulse and photon recirculation technologies in the treatment of patients suffering from erythematous and papulous subtypes of rosacea. Materials and methods. Sixty rosacea patients (21 male and 39 female) at the age of 25-65 including 38 patients with erythematous and 22 patients with papulous subtypes of rosacea underwent treatment using broadband pulse light emission (5-8 treatment sessions). Results. After one month of treatment with broadband pulse light emission using smooth pulse and photon recirculation technologies: 68.4% of patients with the erythematous subtype had clinical recovery, and 31.6% - improvement; 27.2% of patients with the papulous subtype - clinical recovery, 54.5% - improvement, and 18.3% had no effect. After 5 months of treatment most of the patients still had a positive effect of the therapy, yet 21.1% of patients with the erythematous subtype and 11% of patients with the papulous subtype had single telangiectasias, increased erythema and single papules. After 10 months of treatment 44.7% of patients with the erythematous subtype of rosacea had an increase in the number of enlarged vessels, and 18.3% of patients with the papulous subtype had signs of a recurrence of the disease. Conclusion. The use of broadband pulse light emission with smooth pulse and photon recirculation technologies has a multiple, painless and significant effect on large lesions in the treatment of patients suffering from erythematous and papulous subtypes of rosacea.
Development of an experimental model of pemphigus vulgaris in laboratory animals
Authors: Kubanov A. A. , Karamova A. E. , Rog K. V. , Abramova T. V. , Smolyannikova V. A. , Murashev A. N. , Bondarenko D. A.
Published: February 2022

Pemphigus vulgaris is a chronic autoimmune bullous disease characterized by the formation of blisters on the skin and/ or mucous tunics as a result of acantholysis. To search for new molecular and biological targets, study pathogenetic mechanisms of the disease development and develop new methods of treatment, it is urgent to create an experimental model of pemphigus in laboratory animals reproducing clinical, histological and immunological signs of pemphigus. Goal of the study. To develop an experimental model of pemphigus by injecting IgG produced from the blood serum taken from patients with pemphigus to neonatal mice of the BALB/c inbred line. Results. Accumulated IgG products taken from patients with pemphigus (main groups) and healthy volunteers (control group) were injected intraperitoneally to neonatal mice of the BALB/с in the doses of 10-30 mg per mouse. Clinical, histological and immunomorphological signs of pemphigus were revealed in the mice from the main group, which received intraperitoneal injections of IgG taken from patients with pemphigus in the dose of 30 mg per mouse. No signs of pemphigus were observed in the mice from the control group, which received injections of IgG taken from healthy people. This study confirms the role of pemphigus autoantibodies in the pathogenesis of pemphigus vulgaris and shows that passive transmission of antibodies to laboratory animals is possible.
Using IgM methods in the diagnostics of syphilis
Authors: Rotanov S. V. , Ermatova F. A.
Published: February 2022

The authors present the results of a questionnaire survey conducted in serological laboratories in 60 dermatovenerology enterprises in the subjects of the Russian Federation on the use of up-to-date methods for the determination of Class M antibodies for the diagnostics of early syphilis. It was revealed that treponemal specific Class M antibodies were determined in 86.67% of laboratories: the EIAIgM method was used in 86.67% of laboratories, IBIgM - in 18.33%, and PHTIgM - in 6.67% of laboratories only. The total number of IgM tests did not exceed 1.3% of the entire volume of immunochemical reactions conducted for the diagnostics of syphilis in laboratories in 2012; as for IgM methods, the use of EIAIgM prevailed - 97.27% while the frequency of using PHTIgM and IBIgM was 2.0 and 0.73%, respectively. In the authors’ opinion, such ratios were stipulated by the absence of production of kits made in Russia during the study period (in 2012) and allowed for use for methods such as PHTIgM and IBIgM while there was a wide choice of kits required for EIAIgM and laboratories had a long-term experience of using this method.
Topical therapy of infected corticosteroid-sensitive dermatoses
Authors: Torshina I. E.
Published: February 2022

The article presents the current literature data on the rationale for the inclusion in complex therapy (combined corticosteroid drugs) for patients with steroid-sensitive dermatoses complicated by secondary infection. This article contains information definitive studies of efficacy and safety of combination therapy of infected chronic dermatosis drug Akriderm GC.
Phycoemotional state of the patients with severe acne associated with isotretinoin therapy
Authors: Aripova M. L. , Khardikova S. A.
Published: February 2022

Progression of phycoemotional state of 22 patients with severe acne was assessed based on changes in rates of CADI, APSEA, DSQL. Monotherapy with isotretinoin (Acnecutan) is effective in treating severe acne resistant to therapy providing stable remission which is shown by decrease in rates of CADI, APSEA, DSQL.
Nevoid hypopigmentation as a manifestation of skin mosaicism
Authors: Proshutinskaya D. V. , Tekucheva L. V.
Published: February 2022

A case report of rare genetically specified dyschromia - nevoid hypopimentation - was described. This disease is considered as a manifestation of skin mosaicism with characteristics of clinical presentation. Literature data is given, differential diagnostics issues of this pathology are discussed.
ISSEMINATED SUPERFICIAL ACTINIC POROKERATOSIS IN PATIENT SUFFERING FROM PLAQUE PSORIASIS: A CASE REPORT
Authors: Znamenskaya L. F. , Chikin V. V. , Kappusheva I. A. , Kondrasheva V. V.
Published: February 2022

Monitoring of disseminated superficial actinic porokeratosis (DSAP) in patient suffering from plaque psoriasis treated by PUVA therapy was presented. DSAP is a rare skin disease developing in the result of keratosis disorder caused by solar radiation and appearing as clearly localized ringed or line plates. As patients with DSAP are sensitive to solar radiation, phototherapy is contraindicated for them. In this regard, subcutaneous injections of Methotrexate 10 mg once a week were prescribed to a patient suffering from psoriasis. Psoriatic rashes were regressed after the second injection, instead of them DSAP rashes remained.
Experimental models of leprosy
Authors: Kubanov A. A. , Karamova A. E. , Vorontsova A. A. , Kalinina P. A.
Published: February 2022

Leprosy (Hansen’s disease) is a chronic granulomatous bacterial disease which mainly affects skin and peripheral nervous system. Leprosy is caused by the obligate intercellular pathogen known as Mycobacterium leprae. Creating experimental models of leprosy is associated with serious problems due to biological characteristics of the pathogen. Numerous attempts to develop experimental models on different types of animals resulted in a few reproducible models on mice and nine-banded armadillos. Strains of knockout mice with genetic defects caused by site-directed mutagenesis are used as a basis for different leprosy models. Experimental models of leprosy are used for screening of anti-leprosy drugs, detection of drug resistance, studies on the pathogenesis of leprosy, production and evaluation of viability of M. leprae, developing of anti-leprosy vaccines.
Pathomorphological changes in patients with Jessner>s lymphocytic infiltrate of skin, reticular erythematous mucinosis and lupus tumidus
Authors: Trophymov P. N. , Antonova O. V. , Khairutdinov V. R. , Belousova I. E. , Samtsov A. V.
Published: February 2022

Jessner’s lymphocytic infiltrate of skin, reticular erythematous mucinosis and lupus tumidus are the group of rare, insufficiently explored dermatosis with common clinical and histological features, whose nosology is controversial today. Plasmacytoid dendritic cells play the key role in autoimmune disease induction The target of our study was the analysis of pathomorphological features of lesional skin and the research of plasmacytoid dendritic cells in the skin of patients with Jessner’s lymphocytic infiltrate of skin, reticular erythematous mucinosis and lupus tumidus. Material and methods. Histological and indirect immunohistochemistry examination (anti-CD123 antibodies) of lesional skin of 36 patients was held (JLIS - 12, REM - 10, LT - 14) and 10 healthy people. Result. The main pathomorphological changes of skin in all groups were found in dermis: dense perivascular and perifollicular infiltration and deposit of mucin between collagen fibers. Deposits of mucin in papillary dermis were found between patients with JLIS, REM and LT in 10/12 (83%), 7/10 (70%) and 12/14 (86%) of cases, there were no mucin found in skin of healthy people 0/10 (0%) In all dermatosis the distribution of CD123 cells in lesional skin, in immunohistochemistry study, was corresponded clusters model of infiltrate - plasmacytoid DC concentrate in groups around vessels of superficial and profundus networks and perivascular. The comparative analysis of density and localization of perivascular infiltration, number of CD123-cells and their correspondence in dermis perivascular infiltrates didn’t reveal statistically significant differences between JLIS, REM and LT. Results. Our results allow us to consider JLIS and LT as identical decease, and REM as similar in clinical-pathomorphological features dermatosis.
Psycho-emotional conditions of patients with non-cicatrazing alopecia (circumsribed, androgenetic)
Authors: Mareeva A. N. , Kondrakhina I. N.
Published: February 2022

Most common psycho-emotional disorders are described with patients with circumsribed, androgenetic alopecia. Main identification methods presented. It was shown based on analysis of literary data and in order to upgrade the quality of medical help for this group of patients how important it is to identify the preoccupying, depressive and other pathological psychogenic conditions of patients chronic alopecia.
Application of a preparation of imiquimod in а dosage form of ointment 5% in therapy of basal cell carcinoma
Authors: Zaslavsky D. V. , Chuprov I. N. , Sidikov A. A. , Wolkensteyn PIER , Sadykov A. I. , Sibgatullin R. R. , Koval Y. G. , Skrek S. V.
Published: February 2022

Objective. To estimate efficiency and safety of application of a preparation of Aldara (imiquimod, 5% external application cream) it is independent or in combination with cryotherapy in therapy of the basal cell carcinoma of the skin (BCC). Material and methods. Research included 78 patients (22 women and 56 men) with various forms of a basal cell carcinoma of the skin. All patients were divided into 3 groups: the main group (n = 30) the patients receiving therapy by Aldara (imiquimod, 5% external application cream) made, second (n = 21) - the patients receiving therapy by Aldara’s, and also 1 course of cryotherapy, and the group of comparison (n = 27) receiving therapy of placebo in the form of external application cream. Therapy was carried out 3 times a week, within 16 weeks. Research was conducted in some stages with histologic confirmation of the diagnosis, and also an assessment of efficiency of therapy and undesirable effects. Results. At 27 of 30 patients of the first group (90%) and at 21 of 21 patients of the second group (100%) receiving treatment by Aldara’s absolute clinical recovery is reached. In group of comparison of clinical recovery at all 27 patients it wasn’t observed. It is expedient to note that at all patients of the second group (100%) already to 75 ± to the 2nd day of therapy (the 4th visit) clinical recovery while at 27 of 30 patients of the first group (90%) the total disappearance (clinical recovery) came for 105 and more days (the 5th and 6th visits) was noted. Recurrence of a disease after the end of therapy it wasn’t observed. Conclusion. Aldara (imiquimod, 5% external application cream) possesses high efficiency at therapy of various forms and sizes of BCC. Undesirable effects of therapy are easily resolved at cancellation of a preparation and not observed at its renewal. Unlike other immunomodulatory preparations (corticosteroids, retinoid, cyclosporin) Aldar’s application doesn’t promote suppression of cellular immunity.
Erythemato-telangiectatic rosacea: analysis of effectivity of treatment by flavonoid-rich topical agents
Authors: Adaskevich V. P. , Saprykova V. V. , Adaskevich M. V.
Published: February 2022

Rosacea is a common non-infectious disease with intricate aetiology involving endogenous, systemic or local, and environmental factors. Vascular changes may play a major role in rosacea pathogenesis. Chrysanthellum indicum extract contains a unique combination of component which has a beneficial effect on vascular wall permeability and increases the mechanical resistance of capillaries. The aim of the study is to investigate clinical and epidemiologic features of rosacea as well as to evaluate the efficacy and tolerability of external medication - flavonoid-rich plant extract-based cream Ruboril - in patients with erythemato-telangiectatic subtype of rosacea. Material and methods. We observed 52 patients (16 male and 36 female) aged from 23 to 69 with erythemato-telangiectatic subtype of rosacea. The diagnosis was established on the base of clinical presentation with regard to major and minor diagnostic criteria. In order to evaluate the efficacy of topical therapy with Ruboril cream a scale from 0 to 3 was used assessing the severity grade of erythema and teleangiectasias before treatment and after the 4th, 8th and 12th week of therapy. Results. Women were more often affected than men (69%). Patients of the age group from 40 years prevailed (75%). Fitzpatrick skin phototype II was revealed in 30 (58%) of patients. The most common provoking factors were meteorological (77%), emotional stress (67%), sauna/hot baths or alcohol (66%), hot drinks (48%). The prevailing areas of affection were cheeks (98%), forehead (54%), chin (48%), nose (52%). Moderate erythema was observed in 29 (56%) of patients, severe erythema in 15 (29%). Moderate grade of telangiectasias was registered in 30 (58%) of patients, numerous telangiectasias in 16 (31%). Topical application of cream Ruboril allowed to achieve good immediate results: the severity of erythema was reduced by 63%, the manifestation of telangiectasias decreased by 63%. Conclusion. Clinico-epidemiologic features of patients with erythemato-telangiectatic subtype of rosacea are as follows: erythematous and telangiectatic eruption on the cheeks, nose, and chin, weather and emotional influences, predominance of the female gender, skin phototype II, age > 40 years. The external medication Ruboril showed its high efficacy and safety in the treatment of patients with erythemato-telangiectatic subtype of rosacea which allowed to achieve good immediate results with erythema severity reduction of 63% and the decrease in the manifestation of telangiectasias of 63%.
Pleomorphic rhabdomyosarcoma: a case study
Authors: Kozlova A. V. , Kazakov V. M. , Kokh L. N. , Ruksha T. G.
Published: February 2022

Pleomorphic rhabdomyosarcoma of the skin is a fast progressing tumor with high risk of development of lymphogenous and hematogenous metastasis, low survival rates and complex diagnostics. this clinical case describes the application of typing tumor cells on the basis of immunohistochemistry to establish the nature of the tumor clone neoplasms.
Reasonable selection of a topical therapy for different forms of acne
Authors: Kokhan M. M. , Keniksfest YU. V. , Polishchuk A. I.
Published: February 2022

Modern data of the acne pathogenesis, including the role of Propionibacterium acnes in the early stages of the acne formation and their provocative role in the development of the inflammatory process are presented. Priority importance of topical retinoids and combination products containing fixed combinations adapalene with benzoyl peroxide or antibiotic clindamycin in the acne treatment are shown. There are presented the facts of the effectiveness of Clenzit gel (adapalene 0.1%) and Clenzit-C (adapalene 0.1% and clindamycin phosphate 1.0%) and an algorithm for the choice of drug depending on clinical and morphological features of the skin process that allows to optimize the treatment of patients with various forms of acne.
Particular features of the immune status in microbial eczema and erysipelas patients: a therapeutic method
Authors: Zinatulina G. M. , Fayzullina E. V. , Fazyilov V. H. , Vasilkin D. A.
Published: February 2022

Goal of the study. To study particular features of the immune status in microbial eczema and erysipelas patients in the process of a complex immune modulating therapy with Xymedone®. Materials and methods. the study involved 45 microbial eczema patients and 31 patients with a combined form of microbial eczema and erysipelas (the study group) and healthy subjects (60 and 50 subjects). All of the subjects underwent immunology examinations of their cell and humoral immunity prior to and after the pathogenetic treatment with Xymedone®. The traditional therapy of a combined course of microbial eczema and erysipelas comprised the following basic treatment: third-generation cephalosporins (ceftriaxone): 1.0 g a day for 10 days; second-generation antihistamine drugs (peroral loratadine 10 mg once a day for 10 days) and ascorbic acid 0.5 g a day for 14 days. topical treatment comprised antiseptic agents (furacilin solution 1:1000) at the acute stage, emulsions and creams (depending on the disease stage): topical mometasone furoate 0.1% twice a day for 14 days. The patients receiving Xymedone were treated in the dose of 0.25 g, one pill tree times a day for 14 days. The patients underwent an immunology study using the Sysmex Corporation XT2000 automated analyzer at the CityLab laboratory centers in the city of Kazan. Key findings. Prior to the pathogenic therapy, a high level of CD4 (T helpers) and reduced level of the immunoregulatory index (T helpers to T suppressors ratio) (IRI) was revealed in the microbial eczema patients. Ig А and Ig G concentrations were reliably increased. According to a study of immunology indices, the pathogenic therapy using Xymedone® normalized the number of peripheral blood leucocytes and reliably increased the IRI (p < 0.001) in 45 microbial eczema patients. Prior to the pathogenic therapy, a reduced level of CD3 and CD4 cells was revealed in patients with a combined form of microbial eczema and erysipelas; the levels of CD8 and NK cells (CD16) were also reduced. Ig А, Ig M and IgG concentrations were reliably reduced. According to a study of immunology indices, the pathogenic therapy using Xymedone® increased the concentration of CD3 and CD4 cells. Conclusion. Xymedone®, an immune modulating drug, administered as a part of a combination therapy of microbial eczema patients and patients with a combined form of microbial eczema and erysipelas is a form of supplementary pathogenic I therapy; the treatment improved the cellular and humoral immunity indices.
Organization of primary prevention of infections sexually transmitted among orphans and children without parental care in the conditions of children's social institutions
Authors: Nikolaeva K. I. , Ufimceva M. A. , Syrneva T. A.
Published: February 2022

The article presents the results of a survey held among orphaned children and children without parental care with regard to the prevention of sexually transmitted infections as well as results showing the efficacy of awareness-raising workshops regarding reproductive health.
Resources and organization of specialized medical care on the profile “Dermatovenereology” the branch of OJSC “Russian Railways” West Siberian Railway (ZSZHD)
Authors: Karpova O. A.
Published: February 2022

The paper presents the results of the comparative analysis of dermatovenerologists service density within West-Siberian railway and territorial entities of Siberian Federal District of Russian Federation. To give an overall characteristic of dermatovenerological service at WSR, a complex assessment of occupied positions, number of individual persons, manning level and the portion of certificated doctors is used.