bluuebunny/crossref_metadata_2025 huggingface dataset:
Information utilization pattern among male and female dairy farmers of Punjab
Authors: . Vaishali , Gupta Ritu Mittal
Published: January 2024

The current research was carried out in rural Punjab to study gender desegregated information seeking and utilization behaviour as well as their role in the dairy venture. By using pretested interview schedule, the data were collected from 160dairy farmers from three socio-cultural zones of Punjab. Gender desegregated role performance showed that male dairy farmers were more responsible for animal health care, feeding and breeding while female dairy farmers were majorly responsible for dairy finance and shed cleaning. Dairy farmers relied more upon the personal cosmopolite sources for the information followed by localite sources and impersonal cosmopolite channels. Male dairy farmer significantly sought more information from DDB, Pash uPalan Mela, State Extension Officer, PAU/KVK experts and progressive farmers as compared to females who significantly sought information more from family/relatives. In the utilizationof information, female dairy farmers were ahead of their male counter parts as they significantly utilize more information sought from progressive farmers, family/relatives, localite organisations and co-operative societies. It is eye opening to note that though women sought less information, whatever they sought they utilize. Results offer food for thought to effectively transfer technologies, ideas, practices to grass root level. It is suggested to ensure equal participation of female in various extension programs so that new ideas are readily accepted and utilised by farm families. So, it can be concluded that if new technologies or innovations are transferred effectively to women then the technologies will be more readily acceptable among farm families.
Participatory evaluation of ethno-veterinary livestock health practices byfarmers from surrounding villages in Ranthambore Tiger Reserve, India
Authors: Meena Deepak Chand , Meena BS , Garai Sanchita , Sankhala Gopal
Published: January 2024

The present study was conducted to document the ethno-veterinary practices, 360 livestock farmers from the 30villages surrounding the tiger reserve were interviewed using anopen-ended interview schedule to enlist all the practices against the ailments of livestock from the purposefully chosen zone namely Critical Tiger Habitat and buffer zone of the tiger reserve. For participatory assessment of the identified ethno-veterinary practices, Quantification of Indigenous Knowledge and to identify the best practices among overall practices one way variance followed by DMRT method was applied.Use of Drimiaindica (Roxb.) Jessop was found most effective practice against mastitis and use of seeds of Sorghum halepense L. against thediarrhoea, stem bark of Salodora persica L. for fever, and fruits of Areca catechu for treatment of prolapse in the livestock were found most effective practices. The pharmacodynamics of the see thno-veterinary techniques could therefore be studied before further replication and implementation.
Trends in herbal pharmaceutical patent protection for Dairy industry: Perspectivefrom Grassroots innovations
Authors: Sharma Parul , Rajeshwari R , Agarwal Praanjal , Ravikumar R K
Published: January 2024

The national patent regime had amended Patent Acton innovation as per requirement for protecting pharmaceutical product patents among member states of World Trade Organisation. In this respect, the study was conducted to understand patentability nature and prosecution timelines forpatent protected indigenous dairy technologies. Investigators had examined 28 herbal pharmaceutical patent grant(s) from grassroots innovators/outstanding knowledge holders. Patent applications were filed between 2007-2014, prosecution were held and accorded patent grant during 2019-2022. The study noted that Section 3 (d), (e) and (p) were principal factors involved while defending veterinary pharmaceutical patent applications. About 46 percent did not have Section 3 (d) objections inferring these medicinal practices followed by dairy farmers were unique. It was observed that majority of patent grants were in productcategory illustrating transformation of national patent regime from process patent to product-patent grant system. Overall time taken for availing grant was about ten years with an average prosecution period of 2.6 years. Therapeutic efficacy, synergisticaction and suitable claim amendments are pertinent features in responding to prosecution stages. These pharmaceutical product patents from social innovation are of interest to dairy industry. These experimental wisdom provide cost effective sustainable technologies for dairy health system and repurpose existing knowledge of local communities. It is paramount to nurture this bottom-up approach for improvising sustainable dairy health service delivery system.
Narrativas de professores: saberes profissionais na construção do saber fazer docência
Authors: Teno Neide Araujo Castilho , Bueno Elza Sabino da Silva , Camargo Junior Ivo Di
Published: January 2024

Este texto está fundamentado em estudos acerca da narrativa e da reflexividade das experiências vivenciadas em diferentes contextos, vinculado a um projeto de pesquisa sob o título “Narrativas de Saberes Profissionais: diálogos sobre o agente de letramento e o ensino de línguas”. A finalidade deste artigo é analisar os saberes profissionais na construção do fazer docente por meio de narrativas de professores em tempos de pandemia. Os sujeitos foram três professores regularmente matriculados no Programa de Mestrado Acadêmico em Letras da Universidade Estadual de Mato Grosso do Sul – Campo Grande, com entrevistas semiestruturadas, que serviram de instrumentos de recolha das narrativas. A fundamentação teórica está ancorada nos estudos de Street (2003), Nóvoa (2010) e Passeggi (2021). Trata-se de estudo de natureza descritivo-interpretativa, narrativo, apontando como resultados um panorama de ressignificação de práticas de ensino e a necessidade de investimentos em infraestrutura tecnológica na formação continuada de professores.
Memória e testemunho nas biografias jornalísticas de João Gilberto e Caetano Veloso
Authors: Maia Marta Regina , Santos Júnior Carlos Augusto Pereira dos , Fernandes Elias Costa
Published: January 2024

Os livros biográficos, no Brasil, representam um lugar privilegiado para as histórias de vida de pessoas conhecidas ou anônimas. Temos como objetivo, portanto, neste artigo, refletir sobre o processo de produção das biografias de João Gilberto e Caetano Veloso, ambas escritas por jornalistas, a partir de dois operadores metodológicos, que são a memória e o testemunho. A análise das obras, tendo como referência esses operadores, nos possibilitam indicar que o jornalismo testemunhal amplia as temporalidades do campo, antes visto especialmente pela lógica do tempo presente, e passa a ser também um poderoso agente de memória cultural.
Gut colonization and subsequent infection of neonates caused by extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae
Authors: Jiménez-Rojas Verónica , Villanueva-García Dina , Miranda-Vega Ana Luisa , Aldana-Vergara Rubén , Aguilar-Rodea Pamela , López-Marceliano Beatriz , Reyes-López Alfonso , Alcántar-Curiel María Dolores
Published: January 2024

The gut microbiota harbors diverse bacteria considered reservoirs for antimicrobial resistance genes. The global emergence of extended-spectrum beta-lactamase (ESBL)-producing Enterobacterales (ESBL-PE) significantly contributes to healthcare-associated infections (HAIs). We investigated the presence of ESBL-producing Escherichia coli (ESBL-PEco) and ESBL-producing Klebsiella pneumoniae (ESBL-PKpn) in neonatal patients’ guts. Furthermore, we identified the factors contributing to the transition towards ESBL-PEco and ESBL-PKpn-associated healthcare-associated infections (HAIs). The study was conducted from August 2019 to February 2020, in a Neonatal Intensive Care Unit of the Hospital Infantil de México Federico Gómez. Rectal samples were obtained upon admission, on a weekly basis for a month, and then biweekly until discharge from the neonatology ward. Clinical data, culture results, and infection information were gathered. We conducted antimicrobial tests, multiplex PCR assay, and pulsed-field gel electrophoresis (PFGE) to determine the antimicrobial resistance profile and genetic relationships. A comparison between the group’s controls and cases was performed using the Wilcoxon and Student t-tests. Of the 61 patients enrolled, 47 were included, and 203 rectal samples were collected, identifying 242 isolates. In 41/47 (87%) patients, colonization was due to ESBL-PEco or ESBL-PKpn. And nine of them developed HAIs (22%, 9/41). ESBL-PEco resistance to cephalosporins ranged from 25.4% to 100%, while ESBL-PKpn resistance varied from 3% to 99%, and both bacteria were susceptible to carbapenems, tigecillin, and colistin. The prevalent blaCTX-M-group-1 gene accounted for 77.2% in ESBL-PEco and 82.2% in ESBL-PKpn, followed by blaTEM 50% and blaOXA-1 43.8% in ESBL-PEco and blaTEM 80.2% and blaSHV 76.2% in ESBL-PKpn. Analysis of clonality revealed identical colonizing and infection isolates in only seven patients. Significant risk factors included hospital stay duration, duration of antibiotic treatment, and invasive device usage. Our findings suggest high ESBL-PEco and ESBL-PKpn rates of colonization often lead to infection in neonates. Attention should be paid to patients with ESBL-PE.
Readily available drugs and other interventions to potentially improve the efficacy of immune checkpoint blockade in cancer
Authors: Coleman Merissa , Mascialino Sophia J. , Panjwani Anusha , Edwards Emily , Sukhatme Vidula V. , Gavegnano Christina , Sukhatme Vikas P.
Published: January 2024

To improve the efficacy of immune checkpoint inhibitors (ICIs) for cancer treatment, various strategies, including combination therapies with repurposed drugs, are being explored. Several readily available interventions with potential to enhance programmed death 1 (PD-1) blockade have been identified. However, these interventions often remain overlooked due to the lack of financial incentives for their development, making them financial orphans. This review summarizes current knowledge regarding off-label drugs, supplements, and other readily available interventions that could improve the efficacy of PD-1 blockade. The summary of each intervention includes the proposed mechanism of action for combination with checkpoint inhibitors and data from animal and human studies. Additionally, we include summaries of common interventions to be avoided by patients on PD-1 blockade. Finally, we present approaches for conducting further studies in patients, with the aim of expediting the clinical development of these interventions. We strive to increase awareness of readily available combination therapies that may advance cancer immunotherapy and help patients today.
Durvalumab with or without tremelimumab for patients with recurrent or metastatic squamous cell carcinoma of the head and neck: a systematic review and meta-analysis
Authors: Han Xiao , Zhang Haidong , Sun Kai , Li Jing , Wu Wanjuan , Liu Kai , Yu Zhenkun
Published: January 2024

Objective

Head and neck squamous cell carcinoma (HNSCC) ranks as the sixth most prevalent cancer worldwide, significantly impacting patients’ quality of life. Immune checkpoint inhibitors (ICI) have been employed in the treatment of recurrent/metastatic (R/M)-HNSCC patients. This meta-analysis aims to assess the efficacy and safety of durvalumab monotherapy compared to the combination of durvalumab and tremelimumab in R/M-HNSCC patients.

Methods

Relevant studies were systematically searched in PubMed, Embase, and Cochrane Library databases. All articles comparing durvalumab monotherapy with the combination with durvalumab and tremelimumab in R/M-HNSCC treatment were included. Additionally, the references of identified studies were screened if necessary.

Result

A total of 1298 patients from three studies comparing durvalumab with durvalumab and tremelimumab in treating R/M-HNSCC were include in this meta-analysis. Our findings revealed no significant difference in objective response rate (ORR) [odds ratio (OR): 1.15, 95% confidence interval (CI): 0.85 to 1.56, P = 0.36] and disease control rate (DCR) (OR=1.08, 95%CI: 0.86 to 1.37, P = 0.51). Similar outcomes were observed in overall survival (OS), progression-free survival (PFS), and duration of response (DoR). Regarding safety, there was no significant difference in the incidence of treatment-related adverse events (trAEs) between the two groups (OR=1.26, 95%CI: 0.81 to 1.94, P = 0.30). However, patients treated with the combination therapy exhibited a higher incidence of grade 3-4 trAEs (OR=1.93, 95%CI: 1.36 to 2.73, P = 0.0002) and a greater likelihood of discontinuing treatment due to trAEs (OR=2.07, 95%CI: 1.12 to 3.85, P = 0.02). There was no significant difference in the occurrence of severe trAEs leading to death (OR=1.36, 95%CI: 0.47 to 3.96, P = 0.57).

Conclusion

This meta-analysis suggests that R/M-HNSCC patients receiving the combination of durvalumab and tremelimumab may achieve comparable outcomes in terms of ORR, DCR, OS, PFS, and DoR, without significant differences. However, the combination therapy is associated with a higher incidence of grade 3-4 trAEs and an increased likelihood of treatment discontinuation due to trAEs. These findings highlight the need for cautious consideration of the combination of durvalumab and tremelimumab in R/M-HNSCC patients, which should be further evaluated in high-quality studies.
Causal effects from inflammatory bowel disease on liver function and disease: a two-sample Mendelian randomization study
Authors: Shu Yufeng , Yang Bocheng , Liu Xuanyou , Xu Meihua , Deng Chao , Wu Hao
Published: January 2024

Background

Accumulating evidence has shown that patients with inflammatory bowel disease (IBD) have liver function abnormalities and are susceptible to liver diseases. However, the existence of a causal relationship between IBD and liver function or disease remains unclear.

Methods

A two-sample Mendelian randomization (MR) analysis was performed using genetic associations from publicly available genome-wide association studies (GWAS). These associations encompass ulcerative colitis (UC), Crohn’s disease (CD), liver function traits, and liver disease phenotypes. The liver function traits comprised hepatic biochemistries, percent liver fat, and liver iron content from the UK Biobank. Furthermore, the liver disease phenotypes included cholelithiasis, non-alcoholic fatty liver disease (NAFLD), primary sclerosing cholangitis (PSC), and primary biliary cholangitis (PBC) in cohorts of European ancestry. The primary estimation used the inverse-variance weighted method, with GWAS of C-reactive protein (CRP) in the UK Biobank serving as a positive control outcome.

Results

Genetically predicted UC is causally associated with decreased levels of albumin (ALB) and liver iron content, while genetically predicted CD is causally associated with increased levels of alkaline phosphatase (ALP). Moreover, genetically predicted UC or CD increases the risk of PSC, and CD increases the risk of PBC. Neither UC nor CD causally increases the risk of cholelithiasis and NAFLD.

Conclusion

UC affects the levels of ALB and liver iron content, while CD affects the levels of ALP. Both UC and CD increase the risk of PSC, and CD increases the risk of PBC.
Case report: Rare benign obstructive disease of the biliary tract—a rare case of eosinophilic cholangitis
Authors: Huang Xiao-Ning , Fang Qi-Min , Zhong Yu-Feng , Liu Jing
Published: January 2024

Objective

Eosinophilic cholangitis (EC) is an uncommon form of benign biliary obstruction. It frequently eludes accurate clinical diagnosis, leading to inappropriate treatment methods. It is our aspiration that this clinical report will impart comprehensive insights into EC and, specifically, the critical role of tomographic examination.

Case summary

A 34-year-old man was urgently admitted to the hospital due to excruciating abdominal distress persisting for several hours. Following a six-day course of anti-inflammatory therapy, his symptoms displayed marginal improvement, prompting his discharge. He returned to the hospital a month later for re-examination on doctor’s orders. Based on the results of the re-examination, the patient refused steroid hormone shock therapy and subsequently underwent laparoscopic left-lateral hepatic lobectomy in order to confirm the diagnosis. The preoperative absolute counts of eosinophils in the peripheral blood were documented as 2.3 × 109/L, 3.06 × 109/L, and 1.50 × 109/L consecutively; concurrently, the corresponding percentages of eosinophils were quantified at levels of 21.90%, 30.70%, and 19.20%. The subsequent postoperative pathological assessment unveiled EC as the definitive diagnosis. The patient has since remained free from disease recurrence and is presently alive.

Conclusion

When encountering a patient presenting with persistent elevation in absolute eosinophil count in peripheral blood, coupled with imaging manifestations suggestive of intrahepatic periductal inflammation, diagnosis of EC should be highly suspected. The most optimal diagnostic and therapeutic workflow for EC could entail CT-guided liver lesion biopsy, ensued by glucocorticoid pulse therapy, and finally, short-term monitoring utilizing CT or MRI (including T1WI, T2WI, DWI, CEMRI) techniques.
Effective microorganisms input efficiently improves the vegetation and microbial community of degraded alpine grassland
Authors: Li Jinsheng , Wei Juping , Shao Xinqing , Yan Xinhui , Liu Kesi
Published: January 2024

Soil beneficial microorganism deficiency in the degraded grasslands have emerged as the major factors negatively impacting soil quality and vegetation productivity. EM (effective microorganisms) has been regarded as a good ameliorant in improving microbial communities and restoring degraded soil of agricultural systems. However, knowledge was inadequate regarding the effects of adding EM on the degraded alpine grassland. Four levels of EM addition (0, 150, 200, 250 mL m–2) were conducted to investigate the effects of EM addition on soil properties and microorganisms of degraded alpine grassland. The addition of EM increased aboveground biomass, soil organic carbon, total nitrogen, available phosphorus, and microbial biomass, but decreased soil electric conductivity. Meanwhile, the relative biomasses of gram-negative bacteria decreased, while the ectomycorrhizal fungi and arbuscular mycorrhizal fungi increased after EM addition. The relationship between microbial communities and environmental factors has been changed. The restore effect of EM increased with the increase of addition time. These results indicated that EM addition could be a good practice to restore the health of the degraded alpine grassland ecosystem.
A sequence-based machine learning model for predicting antigenic distance for H3N2 influenza virus
Authors: Li Xingyi , Li Yanyan , Shang Xuequn , Kong Huihui
Published: January 2024

Introduction

Seasonal influenza A H3N2 viruses are constantly changing, reducing the effectiveness of existing vaccines. As a result, the World Health Organization (WHO) needs to frequently update the vaccine strains to match the antigenicity of emerged H3N2 variants. Traditional assessments of antigenicity rely on serological methods, which are both labor-intensive and time-consuming. Although numerous computational models aim to simplify antigenicity determination, they either lack a robust quantitative linkage between antigenicity and viral sequences or focus restrictively on selected features.

Methods

Here, we propose a novel computational method to predict antigenic distances using multiple features, including not only viral sequence attributes but also integrating four distinct categories of features that significantly affect viral antigenicity in sequences.

Results

This method exhibits low error in virus antigenicity prediction and achieves superior accuracy in discerning antigenic drift. Utilizing this method, we investigated the evolution process of the H3N2 influenza viruses and identified a total of 21 major antigenic clusters from 1968 to 2022.

Discussion

Interestingly, our predicted antigenic map aligns closely with the antigenic map generated with serological data. Thus, our method is a promising tool for detecting antigenic variants and guiding the selection of vaccine candidates.
Transcriptional response to mild therapeutic hypothermia in noise-induced cochlear injury
Authors: Rincon Sabatino Samantha , Sangaletti Rachele , Griswold Anthony , Dietrich W. Dalton , King Curtis S. , Rajguru Suhrud M.
Published: January 2024

Introduction

Prevention or treatment for acoustic injury has been met with many translational challenges, resulting in the absence of FDA-approved interventions. Localized hypothermia following noise exposure mitigates acute cochlear injury and may serve as a potential avenue for therapeutic approaches. However, the mechanisms by which hypothermia results in therapeutic improvements are poorly understood.

Methods

This study performs the transcriptomic analysis of cochleae from juvenile rats that experienced noise-induced hearing loss (NIHL) followed by hypothermia or control normothermia treatment.

Results

Differential gene expression results from RNA sequencing at 24 h post-exposure to noise suggest that NIHL alone results in increased inflammatory and immune defense responses, involving complement activation and cytokine-mediated signaling. Hypothermia treatment post-noise, in turn, may mitigate the acute inflammatory response.

Discussion

This study provides a framework for future research to optimize hypothermic intervention for ameliorating hearing loss and suggests additional pathways that could be targeted for NIHL therapeutic intervention.
Dyschromatopsia: a comprehensive analysis of mechanisms and cutting-edge treatments for color vision deficiency
Authors: Yang Zihao , Yan Lin , Zhang Wenliang , Qi Jia , An Wenjing , Yao Kai
Published: January 2024

Color blindness is a retinal disease that mainly manifests as a color vision disorder, characterized by achromatopsia, red-green color blindness, and blue-yellow color blindness. With the development of technology and progress in theory, extensive research has been conducted on the genetic basis of color blindness, and various approaches have been explored for its treatment. This article aims to provide a comprehensive review of recent advances in understanding the pathological mechanism, clinical symptoms, and treatment options for color blindness. Additionally, we discuss the various treatment approaches that have been developed to address color blindness, including gene therapy, pharmacological interventions, and visual aids. Furthermore, we highlight the promising results from clinical trials of these treatments, as well as the ongoing challenges that must be addressed to achieve effective and long-lasting therapeutic outcomes. Overall, this review provides valuable insights into the current state of research on color blindness, with the intention of informing further investigation and development of effective treatments for this disease.
Association of dietary fat intake with skeletal muscle mass and muscle strength in adults aged 20–59: NHANES 2011–2014
Authors: Wang Shijia , Zhang Yu , Zhang Dandan , Wang Fang , Wei Wei , Wang Qiong , Bao Yuanyuan , Yu Kang
Published: January 2024

Background

Sarcopenia, a progressive loss of skeletal muscle mass and strength, needs to initially prevent in the twenties. Meanwhile, there is a lack of research on the effects of fat consumption on skeletal muscle mass and strength in adults aged 20–59. We aimed to assess associations between dietary fat intake and skeletal muscle mass, as measured by appendicular lean mass adjusted for body mass index (ALMBMI), and muscle strength, as represented by handgrip strength adjusted for body mass index (GSMAXBMI), among adults aged 20–59.

Methods

Dietary fat intake per kilogram of actual body weight was assessed using two 24h recalls, while ALM and GSMAX were measured using DXA and a handgrip dynamometer, respectively. A weighted multiple linear regression model was employed to analyze the association between dietary fat intake and skeletal muscle mass, utilizing data from the National Health and Nutrition Examination Survey spanning from 2011 to 2014. To assess the non-linear relationship and saturation value between dietary fat intake and skeletal muscle mass, a smooth curve fitting approach and a saturation effect analysis model were utilized.

Results

The study comprised a total of 5356 subjects. After adjusting for confounding factors, there was a positive association observed between dietary fat intake and ALMBMI as well as GSMAXBMI. The relationship between dietary fat intake and ALMBMI showed an inverted U-shaped curve, as did the association with GSMAXBMI. Turning points were observed at 1.88 g/kg/d for total fat intake and ALMBMI, as well as at 1.64 g/kg/d for total fat intake and GSMAXBMI. Furthermore, turning points were still evident when stratifying by gender, age, protein intake, and physical activity. The turning points were lower in individuals with low protein intake(<0.8 g/kg/d) and high levels of physical activity.

Conclusion

The moderate dietary fat intake can be beneficial for muscle mass and strength in adults aged 20–59 under specific conditions. Special attention should be directed toward the consumption of fats in individuals with low protein intake and those engaged in high levels of physical activity.
Histopathology image classification: highlighting the gap between manual analysis and AI automation
Authors: Doğan Refika Sultan , Yılmaz Bülent
Published: January 2024

The field of histopathological image analysis has evolved significantly with the advent of digital pathology, leading to the development of automated models capable of classifying tissues and structures within diverse pathological images. Artificial intelligence algorithms, such as convolutional neural networks, have shown remarkable capabilities in pathology image analysis tasks, including tumor identification, metastasis detection, and patient prognosis assessment. However, traditional manual analysis methods have generally shown low accuracy in diagnosing colorectal cancer using histopathological images. This study investigates the use of AI in image classification and image analytics using histopathological images using the histogram of oriented gradients method. The study develops an AI-based architecture for image classification using histopathological images, aiming to achieve high performance with less complexity through specific parameters and layers. In this study, we investigate the complicated state of histopathological image classification, explicitly focusing on categorizing nine distinct tissue types. Our research used open-source multi-centered image datasets that included records of 100.000 non-overlapping images from 86 patients for training and 7180 non-overlapping images from 50 patients for testing. The study compares two distinct approaches, training artificial intelligence-based algorithms and manual machine learning models, to automate tissue classification. This research comprises two primary classification tasks: binary classification, distinguishing between normal and tumor tissues, and multi-classification, encompassing nine tissue types, including adipose, background, debris, stroma, lymphocytes, mucus, smooth muscle, normal colon mucosa, and tumor. Our findings show that artificial intelligence-based systems can achieve 0.91 and 0.97 accuracy in binary and multi-class classifications. In comparison, the histogram of directed gradient features and the Random Forest classifier achieved accuracy rates of 0.75 and 0.44 in binary and multi-class classifications, respectively. Our artificial intelligence-based methods are generalizable, allowing them to be integrated into histopathology diagnostics procedures and improve diagnostic accuracy and efficiency. The CNN model outperforms existing machine learning techniques, demonstrating its potential to improve the precision and effectiveness of histopathology image analysis. This research emphasizes the importance of maintaining data consistency and applying normalization methods during the data preparation stage for analysis. It particularly highlights the potential of artificial intelligence to assess histopathological images.
Efficacy and safety analysis of TACE + PEI + lenvatinib compared with TACE + lenvatinib for the treatment of hepatocellular carcinoma with PVTT: a retrospective study
Authors: Lu Haohao , Zheng Chuansheng , Liang Bin , Xia Xiangwen , Fan Hongjie
Published: January 2024

Objective

The aim of this study was to investigate the efficacy and safety of transcatheter arterial chemoembolization (TACE) combined with percutaneous ethanol injection (PEI) and lenvatinib in HCC patients with PVTT (Vp2-3), thus providing a safe and effective treatment strategy for advanced HCC patients.

Materials and methods

Clinical data of 227 patients with unresectable HCC and PVTT treated at the Union Hospital from January 2018 to December 2021 were retrospectively analyzed. The patients were divided into two groups according to their treatment methods: TACE+PEI+lenvatinib group (N=103) and TACE+lenvatinib group (N=124).

Results

The proportion of patients with disappearance, shrinkage, or no change of PVTT after treatment was significantly higher in the TACE+PEI+lenvatinib group compared to the TACE+lenvatinib group, with statistical significance (P<0.001). The TACE+PEI+lenvatinib group had higher objective response rate (ORR) (50.5% vs. 25.8%, P<0.001) and disease control rate (DCR) (87.4% vs. 74.2%, P=0.013) than the TACE+lenvatinib group. The median progression-free survival (mPFS) of the TACE+PEI+lenvatinib group was longer than that of the TACE+lenvatinib group (8.1 months vs. 6.5 months, P<0.001). Consistently, the median overall survival (mOS) of the TACE+PEI+lenvatinib group was longer than that of the TACE+lenvatinib group (17.1 months vs. 13.9 months, P<0.001).

Conclusion

Among HCC patients with PVTT (Vp2-3), TACE+PEI+lenvatinib is more effective comparing to TACE+lenvatinib in prolonging PFS and OS. The control of PVTT in the TACE+PEI+lenvatinib group was significantly more satisfactory than that in the TACE+lenvatinib group. TACE+PEI+lenvatinib is a safe and effective treatment strategy for HCC patients with PVTT (Vp2-3).
Establishment of feijoa (Acca sellowiana) callus and cell suspension cultures and identification of arctigenin - a high value bioactive compound
Authors: Raikar Sanjeev V. , Isak Ilena , Patel Sunita , Newson Harriet L. , Hill Stefan J.
Published: January 2024

Feijoa (Acca sellowiana (O. Berg.) Burret), also known as pineapple guava, is a member of the Myrtaceae family and is well known for its fruit. Chemical profiling of the different tissues of the feijoa plant has shown that they generate an array of useful bioactive compounds which have health benefits such as significant antioxidant activities. In this study, an in vitro culture system has been developed, which could be explored to extract high-value bioactive compounds from feijoa. Feijoa tissue culture was initiated by the induction of callus from floral buds. Sections of floral buds were plated on MS medium supplemented with 2,4-D and BAP at 2.0mg/L and 0.2mg/L concentrations, respectively. Cell suspension cultures of feijoa were established using a liquid MS medium with different concentrations of 2,4-D and BAP and cultured on a rotary shaker. The growth of the cell suspension was evaluated with different parameters such as different carbohydrate sources, concentration of MS media, and inoculum density. When the cell suspensions were treated with different concentrations of MeJA at different time points, phytochemicals UPLC - QTOF MS analysis identified extractables of interest. The main compounds identified were secondary metabolites (flavonoids and flavonoid-glucosides) and plant hormones. These compounds are of interest for their potential use in therapeutics or skin and personal care products. This report investigates essential methodology parameters for establishing cell suspension cultures from feijoa floral buds, which could be used to generate in vitro biomass to produce high-value bioactive compounds. This is the first study reporting the identification of arctigenin from feijoa, a high-value compound whose pharmaceutical properties, including anti-tumour, anti-inflammatory and anti-colitis effects, have been widely reported. The ability of feijoa cell cultures to produce such high-value bioactive compounds is extremely promising for its use in pharmaceuticals, cosmeceuticals and nutraceuticals applications.
Trends in waking salivary alpha-amylase levels following healing lucid dreams
Authors: Yount Garret , Taddeo Sitara , Stumbrys Tadas , Kriegsman Michael , Wahbeh Helané
Published: January 2024

Introduction

Salivary alpha-amylase (sAA) is considered a marker of autonomic nervous system activity in stress research, and atypical waking sAA responses have been reported for traumatized individuals. Lucid dreams, characterized by a dreamer’s awareness of their dream state while remaining asleep, have shown promising preliminary evidence of their potential to enhance mental health. This study’s objective was to evaluate sAA in relation to healing lucid dreams.

Methods

Participants experiencing PTSD symptoms attended a six-day workshop delivered via live video designed to teach techniques for transforming trauma through dreamwork and dream lucidity. Participants (n = 20) collected saliva samples each morning, immediately upon awakening (Time 1) and 30 min afterward (Time 2). sAA levels were determined by enzymatic assay, and the waking sAA slope was calculated as the difference of Time 2 minus Time 1. Participants completed dream reports each morning, with a dream classified as a ‘healing lucid dream’ when they reported attaining lucidity and remembered their intention to manifest a healing experience within the dreamscape.

Results

Of eight participants experiencing healing lucid dreams, four were able to provide usable saliva samples. Statistical tests on these four participants were not significant because of low power. However, nonsignificant positive associations were observed between experiencing more healing lucid dreams and increased waking sAA slope.

Conclusion

The results did not reveal a consistent effect of healing lucid dreams on waking sAA slope. Identifying meaningful patterns in this relationship will require larger samples and more stringent control over saliva collection procedures in future studies.
The prevalence of off-label use and supratherapeutic blood levels of outpatient psychotropic medication in suicidal adolescents
Authors: Hach Isabel , Bertsch Thomas , Nonell Patrick
Published: January 2024

Introduction

Adolescents with mental disorders show an increased risk of suicidal phenomena. Vice versa, suicidality is a serious adverse event of psychotropic drug therapy in adolescents. There are only a few new psychotropic agents approved for this young age group. We evaluated the (pre-pandemic) prevalence of off-label use as well as detailed blood concentrations of outpatient psychotropic medication and sex differences in a clinical population of suicidal adolescents.

Methods

The urine presence and serum levels of psychotropic substances of adolescents hospitalized due to their acute suicidality but without a known actual suicide attempt (i.e., no acute intoxication or serious self-injuries) were investigated routinely between 01.03.2017 and 31.01.2018. Urine (N = 205) and blood samples (N = 193) were taken at the beginning of closed inpatient admission, i.e., the results of the laboratory analysis reflect outpatient drug intake. The serum levels of psychopharmacological medication and OTC medication were measured.

Results

Our sample consists of 231 cases (boys: N = 54; girls: N = 177, ratio: 1:3.3), aged 12–17 years (average age: 15,4 years). The most prevalent psychiatric diagnoses were depressive episodes (54%) and adjustment disorders (25%), and girls were more often diagnosed with depressive disorders than boys (boy/girl ratio: 1:9.5, p < 0.0001). More than half of adolescents (56%) used at least one prescribed psychotropic drug at admission (24.8% ≥ two psychotropic drugs). Off-label use of second-generation antipsychotics was significantly more frequent than off-label use of antidepressants (85% vs. 31%, p < 0.01). Adolescents suffering from depressive disorders were significantly more often on-label treated than adolescents with neurotic or stress-related disorders (56% vs. 10%). Female cases with prescribed psychotropic drug use showed significantly more frequent supratherapeutic drug levels than male cases (5% vs. 27%, p < 0.05).

Conclusion

Female adolescents may have an increased risk of supratherapeutic blood levels, especially when outpatient prescribed psychotropic drugs are off-label used. Measurement of blood levels of outpatient-prescribed psychotropic drugs could be used to enhance the safety and efficacy of the individual psychopharmacological treatment of adolescent suicidal patients. There is an urgent need for more real-world evidence on the effective treatment of adolescents with psychotropic drugs.
Brain oxygen responses induced by opioids: focus on heroin, fentanyl, and their adulterants
Authors: Kiyatkin Eugene A. , Choi Shinbe
Published: January 2024

Opioids are important tools for pain management, but abuse can result in serious health complications. Of these complications, respiratory depression that leads to brain hypoxia is the most dangerous, resulting in coma and death. Although all opioids at large doses induce brain hypoxia, danger is magnified with synthetic opioids such as fentanyl and structurally similar analogs. These drugs are highly potent, act rapidly, and are often not effectively treated by naloxone, the standard of care for opioid-induced respiratory depression. The goal of this review paper is to present and discuss brain oxygen responses induced by opioids, focusing on heroin and fentanyl. In contrast to studying drug-induced changes in respiratory activity, we used chronically implanted oxygen sensors coupled with high-speed amperometry to directly evaluate physiological and drug-induced fluctuations in brain oxygen levels in awake, freely moving rats. First, we provide an overview of brain oxygen responses to physiological stimuli and discuss the mechanisms regulating oxygen entry into brain tissue. Next, we present data on brain oxygen responses induced by heroin and fentanyl and review underlying mechanisms. These data allowed us to compare the effects of these drugs on brain oxygen in terms of their potency, time-dependent response pattern, and potentially lethal effect at high doses. Then, we present the interactive effects of opioids during polysubstance use (alcohol, ketamine, xylazine) on brain oxygenation. Finally, we consider factors that affect the therapeutic potential of naloxone, focusing on dosage, timing of drug delivery, and contamination of opioids by other neuroactive drugs. The latter issue is considered chiefly with respect to xylazine, which strongly potentiates the hypoxic effects of heroin and fentanyl. Although this work was done in rats, the data are human relevant and will aid in addressing the alarming rise in lethality associated with opioid misuse.
Weekend admissions and outcomes in patients with pneumonia: a systematic review and meta-analysis
Authors: Lu Jiayao , Yang Jing , Cai Xiaofei
Published: January 2024

Background

To document pooled evidence on the association between weekend hospital admissions and the potential risks of mortality, intensive care requirements, and readmission among patients with pneumonia.

Methods

We performed a systematic search across the PubMed, EMBASE, and Scopus databases. We collected observational studies exploring the association between weekend admissions and outcomes of interest in patients with pneumonia. To analyze the data, we used a random effects model and expressed the effect sizes as pooled odds ratios (ORs) accompanied by their respective 95% confidence intervals (CIs).

Results

The analysis comprised data from 13 retrospective studies. Compared to patients admitted on weekdays, those admitted during the weekend had a non-statistically significant marginally higher risk of in-hospital mortality (OR, 1.02; 95% CI, 1.00, 1.04) but similar 30-day mortality after admission (OR, 1.03; 95% CI, 0.97, 1.10), and similar risks of admission to intensive care unit (OR, 1.04; 95% CI, 0.98, 1.11) and re-admission (OR, 0.85; 95% CI, 0.65–1.12).

Conclusion

Our findings do not support the presence of a “weekend effect” in patients with pneumonia.

Systematic review registration

PROSPERO, identifier CRD42023425802, https://www.crd.york.ac.uk/prospero/.
Health poverty alleviation in China from the perspective of historical institutionalism: policy changes and driving factors
Authors: Xu Li , You Xiaojian , Cui Yinan , You Jiali
Published: January 2024

Health poverty alleviation is an effective tool for improving the living quality and developmental conditions of impoverished populations. Since 1978, China has been actively implementing health poverty alleviation projects, resulting in a more robust rural healthcare service network and increased convenience for the local population to access medical treatment. However, it is crucial to acknowledge that China still faces a complex situation with the simultaneous existence of multiple disease threats and the interweaving of various health influencing factors. Ongoing risks of emerging infectious diseases persist, and some previously controlled or eliminated infectious diseases are at risk of resurgence. The incidence of chronic diseases is on the rise and exhibits a trend toward affecting younger populations. Therefore, examining the successful experiences of China's health poverty alleviation over the past 40 years becomes a critically important issue. The study focuses on China's health poverty alleviation policies, employing historical institutionalism as a theoretical perspective to analyze the historical changes and evolutionary logic of health poverty alleviation policies. A historical institutionalist analytical framework for health poverty alleviation policies is constructed. The research findings reveal that China's health poverty alleviation policy has undergone three distinct periods since 1978: the initial phase (1978–2000), the exploratory phase (2000–2012), and the stable development phase (2013–present). At the macro level, the political, economic, and social contexts of different periods have influenced the evolution of health poverty alleviation policies. On the meso level, coordination effects and adaptive expectations have had an impact on China's health poverty alleviation policy. At the micro level, various actors, including the central government, local governments at different levels, social forces, and impoverished communities, interact during the evolution of health poverty alleviation policies. This paper summarizes the theoretical aspects of China's health poverty alleviation policy experience. The research conclusions, viewed through the lens of historical institutionalism, offer practical insights into the evolution of government policies. This provides directional guidance for enhancing health poverty alleviation projects.
Moving beyond western methods: a methodological toolbox for family entrepreneurship research in tourism by including children's voices
Authors: Ju Xiaoxi , Wengel Yana , Schänzel Heike , Liu Claire
Published: January 2024

Recently, tourism scholars turned their attention to families, specifically children's experiences. Yet, research illustrating children's voices in tourism family entrepreneurship is missing. Social researchers are encouraged to include children's voices to reveal their lived experiences rather than considering them too vulnerable to be interviewed. This qualitative study, underpinned by constructivist epistemology, explored how families are embedded within lifestyle migration and the tourism entrepreneurial process on Hainan Island in China. A combination of methods was adopted to create a toolbox suitable for family research, including children's voices through whole-family interviews and LEGO® Serious Play® workshops. Playing LEGO® seriously ensures that the researcher does not drive participants' thoughts, and children can freely express their opinions in playful, metaphorical, and meaningful ways. Moving beyond Western-centered methods, data was collected at Old Dad Teahouses (or Lao Ba Cha 老爸茶 in Chinese) to create a friendly environment. Old Dad Teahouses are a Hainanese cultural ritual where locals gather to enjoy tea along with local savory snacks. Historically, the name Old Dad Tea refers to predominantly male customers over 50 years of age who regularly attended tea houses in the afternoon as part of their leisure. Nowadays, people who go to Old Dad Tea are more diverse in age and gender, and spending time there represents a popular leisure activity among families living in Hainan. We emphasize that our methodological toolbox allows us to explore how individuals construct their understanding through their own belief systems and culture. The methodological toolbox allowed us to understand the scholarship on family tourism entrepreneurship from a Chinese cultural perspective by providing insight into the experiences of 15 children from eight entrepreneurial families, providing agency to the children. This study aims to enrich the definition of family entrepreneurship by identifying how children as family members can influence migration and entrepreneurial behaviors and exploring the experiences gained by children through the entrepreneurial process. Children's voices are usually filled by adults within the family business unit. However, children are also rights holders and social agents. This study supports the right of children to participate and have their voices heard.
The impact of food safety regulatory information intervention on enterprises’ production violations in China: a randomized intervention experiment
Authors: Zhao Tong , Li Taiping , Liu Dan , Luo Yun
Published: January 2024

The prevalence of unsafe food poses a widespread challenge across numerous nations. Despite the continuous investments by the Chinese government in food safety regulation, the condition of food safety in China is still not ideal and requires substantial enhancements. Cost-effective, information-based strategies are essential for the effective management of food safety hazards. In this research, we established an extensive database of food enterprises with documented violations and carried out a randomized intervention trial to assess the effects of regulatory information interventions on the decrease of production violations in these enterprises. The findings reveal that interventions based on food safety regulatory information were instrumental in diminishing production violations among food enterprises and had spillover effects within a given geographic area. It is important to note that the impact of the intervention was delayed, with noticeable results on production violations becoming apparent 6 months post-intervention. Additionally, the degree of information communication and the degree of information concern can positively moderate the reduction of food enterprises’ production violation behavior by food safety regulatory information intervention.