bluuebunny/crossref_metadata_2025 huggingface dataset:
Adjunctive Application of Hyaluronic Acid in Combination with a Sodium Hypochlorite Gel for Non-Surgical Treatment of Residual Pockets Reduces the Need for Periodontal Surgery—Retrospective Analysis of a Clinical Case Series
Authors: Diehl Daniel , Friedmann Anton , Liedloff Pheline , Jung Rico Marvin , Sculean Anton , Bilhan Hakan
Published: September 2022

The comprehensive treatment of periodontitis stage 2 to 4 aims at the resolution of periodontal inflammation and “pocket closure”, which implies a residual probing depth of ≤4 mm and a negative BoP. However, supportive periodontal therapy (SPT) regularly leaves behind persistent periodontal pockets with 5 or more mm in residual PPD and sites that often re-colonize and re-infect. Various adjunctive options for subgingival instrumentation have been proposed to enhance the antimicrobial effects to better control the re-infection of these residual sites. The locally applied adjuncts, based on their anti-inflammatory effect, are sodium hypochlorite antiseptic cleaning gel and cross-linked hyaluronic acid (xHyA). Both recently moved into the focus of clinical research on non-surgical and surgical therapy for periodontitis. The surgical use of xHyA indicates regenerative potential, supporting periodontal regeneration. This case series retrospectively analyzes the clinical benefits of the consecutive flapless application of sodium-hypochlorite-based cleaning gel and xHyA at the SPT to achieve pocket closure, thereby reducing the need for periodontal surgery. In 29 patients, 111 sites received the treatment sequence. At 6-month re-evaluation, an overall PPD reduction exceeding 2 mm was achieved, associated with a similar CAL gain (2.02 mm); the bleeding tendency (BoP) was reduced by >60%. Pocket closure occurred in almost 25% of all the sites. Within their limits, the present data suggest that the proposed combined adjunctive treatment of residual active periodontal sites yielded significant improvement in the clinical parameters. Further studies in RCT format are required to confirm these observations.
High-Performance pH Sensor Electrodes Based on a Hexagonal Pt Nanoparticle Array-Coated Nanoporous Alumina Membrane
Authors: Altowyan Abeer S. , Shaban Mohamed , Gamel Asmaa , Gamal Ahmed , Ali Mona , Rabia Mohamed
Published: September 2022

Porous anodic alumina membranes coated with Pt nanoparticles (PAAM/Pt) have been employed as pH sensor electrodes for H+ ion detection. The PAAM was designed using a two-step anodization process. Pt nanoparticles were then sputtered onto the membrane at different deposition times. The membrane’s morphological, chemical, and optical characteristics were carefully assessed following the fabrication stage using a variety of analytical techniques. The potential of the PAAM/Pt sensor electrode was investigated by measuring the potential using a simple potentiometric method. The effects of depositing Pt nanoparticles for 3–7 min on sensor electrode sensitivity were examined. The optimal potentiometric Nernstian response slope for the PAAM/Pt sensor electrode with 5 min Pt sputter coating is 56.31 mV/decade in the pH range of 3.0 to 10 at 293 K. Additionally, the PAAM/Pt sensor electrode’s stability and selectivity in various ions solutions were examined. The sensor electrode had a lifetime of more than six weeks and was kept in a normal air environment.
Comparison of Structural Analysis of Thin-Walled Structures Accomplished by Isogeometric Analysis and the Finite Element Method
Authors: Bocko Jozef , Pleško Patrik , Delyová Ingrid , Sivák Peter
Published: September 2022

Isogeometric analysis (IGA) represents a relatively new method of problem-solving in engineering practice. A huge advantage of this method over the finite element method (FEM), is the reduction of the simulation execution time. Non-uniform rational B-splines (NURBS) allow the use of higher-order basis functions, thus increasing the accuracy of the solution. This paper deals with the comparison of structural analysis of thin-walled structural elements using isogeometric analysis and the finite element method. The investigated objects are modelled using a single patch in MATLAB. The basic functions are created from NURBS, which were previously used in the creation of an accurate geometric model. The paper contains a comparison of the results obtained by the above-mentioned methods. All computations are performed in the elastic domain.
Antioxidative and Photo-Induced Effects of Different Types of N-Doped Graphene Quantum Dots
Authors: Jovanovic Svetlana , Bonasera Aurelio , Dorontic Sladjana , Zmejkoski Danica , Milivojevic Dusan , Janakiev Tamara , Todorovic Markovic Biljana
Published: September 2022

Due to the increasing number of bacterial infections and the development of resistivity toward antibiotics, new materials and approaches for treatments must be urgently developed. The production of new materials should be ecologically friendly considering overall pollution with chemicals and economically acceptable and accessible to the wide population. Thus, the possibility of using biocompatible graphene quantum dots (GQDs) as an agent in photodynamic therapy was studied. First, dots were obtained using electrochemical cutting of graphite. In only one synthetic step using gamma irradiation, GQDs were doped with N atoms without any reagent. Obtained dots showed blue photoluminescence, with a diameter of 19–89 nm and optical band gap of 3.23–4.73 eV, featuring oxygen-containing, amino, and amide functional groups. Dots showed antioxidative activity; they quenched •OH at a concentration of 10 μg·mL−1, scavenged DPPH• radicals even at 5 μg·mL−1, and caused discoloration of KMnO4 at 30 μg·mL−1. Under light irradiation, dots were able to produce singlet oxygen, which remained stable for 10 min. Photoinduced effects by GQDs were studied on several bacterial strains (Listeria monocytogenes, Bacillus cereus, clinical strains of Streptococcus mutans, S. pyogenes, and S. sangunis, Pseudomonas aeruginosa, and one yeast strain Candida albicans) but antibacterial effects were not noticed.
Impact of Cross-Linked Hyaluronic Acid on Osteogenic Differentiation of SAOS-2 Cells in an Air-Lift Model
Authors: Nobis Bianca , Ostermann Thomas , Weiler Julian , Dittmar Thomas , Friedmann Anton
Published: September 2022

The aim of this study was to investigate the impact of cross-linked hyaluronic acid on osteoblast-like cells seeded on top of two collagen substrates, native porcine pericardium membrane (substrate A) and ribose cross-linked collagen membranes (substrate B), in an air-lift model. Substrates A or B, saturated with three hyaluronic acid concentrations, served as membranes for SAOS-2 cells seeded on top. Cultivation followed for 7 and 14 days in the air-lift model. Controls used the same substrates without hyaluronic pre-treatment. Cells were harvested, and four (Runx2, BGLAP, IBSP, Cx43) different osteogenic differentiation markers were assessed by qPCR. Triplicated experiment outcomes were statistically analyzed (ANOVA, t-test; SPSS). Supplementary histologic analysis confirmed the cells’ vitality. After seven days, only few markers were overexpressed on both substrates. After 14 days, targeted genes were highly expressed on substrate A. The same substrate treated with 1:100 diluted xHyA disclosed statistically significant different expression level vs. substrate B (p = 0.032). Time (p = 0.0001), experimental condition as a function of time (p = 0.022), and substrate (p = 0.028) were statistically significant factors. Histological imaging demonstrated vitality and visualized nuclei. We conclude that the impact of hyaluronic acid resulted in a higher expression profile of SAOS-2 cells on substrate A compared to substrate B in an air-lift culture after two weeks.
A Two-DOF Active-Passive Hybrid Vibration Isolator Based on Multi-Line Spectrum Adaptive Control
Authors: Yang Qingchao , Ma Zhaozhao , Zhou Ruiping
Published: September 2022

In order to effectively control the low-frequency vibration of ship machinery, based on the improved multi-line spectrum adaptive control algorithm, a two-degree-of-freedom (two-DOF) active-passive hybrid vibration isolator composed of an electromagnetic actuator, rubber spring, and the hydraulic device is proposed. The dynamic model of the two-DOF vibration isolation system is established and the main control force demand of the vibration isolation system at different damping forces is analyzed. By introducing the improved wavelet packet decomposition algorithm with the Hartley block least mean square algorithm to the filter-x least mean square (FxLMS) algorithm, an improved wavelet packet Hartley block filter-x least mean square (IWPHB-FxLMS) algorithm is established. The experimental results show that the IWPHB-FxLMS algorithm has better control performance. Compared with the traditional FxLMS algorithm, the IWPHB-FxLMS control algorithm improves the convergence speed by 91.7% and the line spectrum power spectrum attenuation by 58.1%. The active-passive hybrid vibration isolator is based on multi-line spectrum adaptive control and can achieve good control effects under the excitation of multi-frequency line spectrum and constant frequency line spectrum.
Mathematics and Its Applications in Science and Engineering
Authors: Queiruga-Dios Araceli , Santos Sánchez María Jesus , Yilmaz Fatih , Dias Rasteiro Deolinda M. L. , Martín-Vaquero Jesús , Gayoso Martínez Víctor
Published: September 2022

This book contains the successful submissions [...]
Sizing and Sitting of Battery Energy Storage Systems in Distribution Networks with Transient Stability Consideration
Authors: Yang Nien-Che , Zhang Yong-Chang , Adinda Eunike Widya
Published: September 2022

In this study, the capacity and location of battery energy storage systems (BESSs) in a distribution network were evaluated to increase the stability and reliability of power systems by applying the proposed transient stability indicators. The search capability of particle swarm optimization (PSO) combined with Pareto optimality in MATLAB R2019b was used to solve multi-objective problems in steady-state conditions, including active power loss, voltage deviation, and total operating cost. The BESS capacity and location were then derived using the Manhattan distance method. Subsequently, the BESS control model was constructed using the commercial software DIgSILENT PowerFactory 2021 to evaluate generator power smoothing and short-term voltage stability index during the transient response and determine the final capacity and location of BESS in distribution networks. Finally, the accuracy of the proposed method was verified by considering the daily load and solar power generation curves using the IEEE 33-bus radial power distribution network.
Membrane BioReactor (MBR) Activated Sludge Surrogate Alternatives Carboxymethyl Cellulose and Xanthan Gum: A Statistical Analysis and Review
Authors: Ratkovich Nicolás , Amaya-Gómez Rafael
Published: September 2022

Membrane Bioreactors (MBR) combine traditional biological treatments such as Activated Sludge (AS) with a membrane-based filtration process to extract suspended and organic solids. MBR operation involves high shear rates near the membrane surface due to the high crossflow velocity, which complicates any simulation process from a hydrodynamic point of view. In this regard, the viscosity as a function of total suspended solids (TSS) plays an essential role in characterizing and modeling the behavior of activated sludge (AS). However, AS has an intransparency property that prevents experimental measurements (i.e., velocity profiles) commonly associated with optical techniques from being peformed. In light of this limitation, two polymeric compounds, carboxymethyl cellulose (CMC) and xanthan gum (XG), are considered here in order to explore the possibility of mimicking the rheological behavior of AS. These compounds are commonly used in the food industry as food thickeners, and their rheological behavior is supposedly well defined in the literature. In this work, we reviewed the viscosity behavior of these compounds through their reported flow behavior and consistency indexes. It was found that the rheological properties of these two polymers differ depending on the chemical manufacturer, rheometers, and measurement protocols involved. Different curves (shear rate vs. viscosity/shear stress) are obtained, as each device and procedure seem to modify the polymer structure. Therefore, a statistical analysis was performed based on the flow and consistency indexes using different concentrations and temperatures reported in experimental data. Several insights regarding CMC, XG, and AS performance were obtained, including a better relationship with concentration than with temperature or certain exponential-based performances, which can support further MBR design and operational decision-making.
Clarification of Limed Sugarcane Juice by Stainless Steel Membranes and Membrane Fouling Analysis
Authors: Du Nan , Pan Lili , Liu Jidong , Wang Lijun , Li Hong , Li Kai , Xie Caifeng , Hang Fangxue , Lu Haiqin , Li Wen
Published: September 2022

The performance of stainless steel membranes with pore sizes of 100 and 20 nm in clarifying limed sugarcane juice was investigated under different operating conditions. An increase in transmembrane pressure (TMP) for the 20 nm membrane from 2 to 5 bar led to an increase in the average flux from 146.6 Lm−2 h−1 to 187.8 Lm−2 h−1 (approximately 9 h). The increase in crossflow velocity from 2 to 5 m/s led to an increase in the average flux from 111.9 Lm−2 h−1 to 158.1 Lm−2 h−1. The increase in temperature from 70 °C to 90 °C caused an increase in the average flux from 132.8 Lm−2 h−1 to 148.6 Lm−2 h−1. Simultaneously, the test produced a high-quality filtered juice with an average of 1.26 units of purity rise. The purity increased with time, and a 99.99% reduction in turbidity and an average 29.3% reduction in colour were observed. In addition, four classic filtration mathematical models and scanning electron microscopy (SEM) analyses suggested that cake formation is the main mechanism for flux decline. Fourier transform infrared (FTIR) spectrometry and energy-dispersive X-ray (EDX) spectrometry indicated that organic fouling is the main foulant. This study demonstrates the potential of stainless steel membranes as filters for the clarification of raw sugarcane juice.
Plastic Behavior and Microstructure Heterogeneity of an AA6063-T6 Aluminum Alloy Processed by Symmetric and Asymmetric Rolling
Authors: Muñoz Jairo Alberto , Khelfa Tarek , Duarte Gonzalo Ariel , Avalos Martina , Bolmaro Raúl , Cabrera José María
Published: September 2022

Rolling is one of the most employed industrial processes which can be used at multiple manufacturing stages, allowing different geometries such as plates, rods, profiles, billets, slabs, tubes, and seamless tubes to be obtained. However, rolled products develop anisotropy due to the preferential orientation of crystals in the rolling direction. Thus, some process configurations and different processing parameters (e.g., thickness reduction per rolling pass, deformation routes, roll diameters, and strain rate) have been proposed to deal with the desired anisotropy. In this context, this investigation evaluates and compares the effect of symmetrical and asymmetrical rolling on an aluminum alloy sheet deformed until a 38% thickness reduction using multiple rolling passes. The asymmetrical process displayed larger texture and microstructure gradients across the sheet thickness than the symmetrical one, manifested as more grain refinement and more intense shear texture components close to sheet surfaces. In terms of plastic anisotropy, the visco-plastic self-consistent model predicted higher average anisotropy for the symmetric rolling than the asymmetric process due to a strong combination of recrystallization and deformation texture components. Conversely, the asymmetric process showed lower planar anisotropy values due to the increase in the fraction of shear and deformation texture components near the sheet surfaces, producing a less intense overall texture than the symmetric rolling. The additional shear strain component was mainly responsible for the material strengthening and texture weakening after the asymmetrical rolling process. In addition, the shear strain produced grain refinement, decreased misorientation, and higher dislocation densities than the as-received and symmetrically rolled materials. After asymmetrical rolling, the microstructure and texture showed heterogeneous profiles across the sheet thickness. This gave rise to a heterogeneous grain size refinement, decreased misorientation close to sheet edges, and plastic gradients.
Microstructure Comparison for AlSn20Cu Antifriction Alloys Prepared by Semi-Continuous Casting, Semi-Solid Die Casting, and Spray Forming
Authors: Huang Shuhui , Zhu Baohong , Zhang Yongan , Liu Hongwei , Wu Shuaishuai , Xie Haofeng
Published: September 2022

Antifriction alloys such as AlSn20Cu are key material options for sliding bearings used in machinery. Uniform distribution and a near-equiaxed granularity tin phase are generally considered to be ideal characteristics of an AlSn20Cu antifriction alloy, although these properties vary by fabrication method. In this study, to analyze the variation of the microstructure with the fabrication method, AlSn20Cu alloys are prepared by three methods: semi-continuous casting, semi-solid die casting, and spray forming. Bearing blanks are subsequently prepared from the fabricated alloys using different processes. Morphological information, such as the total area ratio and average particle diameter of the tin phase, are quantitatively characterized. For the tin phase of the AlSn20Cu alloy, the deformation and annealing involved in semi-continuous casting leads to a prolate particle shape. The average particle diameter of the tin phase is 12.6 µm, and the overall distribution state is related to the deformation direction. The tin phase of AlSn20Cu alloys prepared by semi-solid die casting presents both nearly spherical and strip shapes, with an average particle diameter of 9.6 µm. The tin phase of AlSn20Cu alloys prepared by spray forming and blocking hot extrusion presents a nearly equilateral shape, with an average particle diameter of 6.2 µm. These results indicate that, of the three preparation methods analyzed in this study, semi-solid die casting provides the shortest process flow time, whereas a finer and more uniform tin-phase structure may be obtained using the spray-forming process. The semi-solid die casting method presents the greatest potential for industrial application, and this method therefore presents a promising possibility for further optimization.
Vitamin D Alleviates Anxiety and Depression in Elderly People with Prediabetes: A Randomized Controlled Study
Authors: Zaromytidou Evangelia , Koufakis Theocharis , Dimakopoulos Georgios , Drivakou Despina , Konstantinidou Stavroula , Rakitzi Pantelitsa , Grammatiki Maria , Manthou Eleni , Notopoulos Athanasios , Iakovou Ioannis , Gotzamani-Psarrakou Anna , Kotsa Kalliopi
Published: September 2022

Older people are prone to frailness, present poor adherence to pharmacotherapy, and often have adverse drug effects. Therefore, it is important to develop effective and safe interventions to mitigate the burden of anxiety and depression disorders in this population. The aim of this study was to investigate the effect of vitamin D supplementation on the anxiety and depression status of elderly people with prediabetes. Participants were randomly assigned a weekly dose of vitamin D3 of 25,000 IU (n = 45, mean age 73.10 ± 7.16 years) or nothing (n = 45, mean age 74.03 ± 7.64 years), in addition to suggested lifestyle measures. The State-Trait Anxiety Inventory subscales (STAI-T and STAI-S) and the Patient Health Questionnaire-9 (PHQ-9) were used to evaluate anxiety and depression levels, respectively, at baseline, 6, and 12 months. A total of 92.68% of the participants in the vitamin D group and 97.14% of the controls exhibited vitamin D deficiency (<20 ng/mL) at baseline. Mean STAI-T scores were lower in supplemented individuals than in the control group at 6 (38.02 ± 9.03 vs. 43.91 ± 7.18, p = 0.003) and 12 months (32.35 ± 7.77 vs. 44.97 ± 7.78, p < 0.001). The same pattern was evident for STAI-S scores at 6 (37.11 ± 7.88 vs. 43.20 ± 9.33, p = 0.003) and 12 months (32.59 ± 6.45 vs. 44.60 ± 9.53, p < 0.001). Supplemented participants demonstrated lower mean PHQ-9 scores compared to controls at 6 (15.69 ± 6.15 vs. 19.77 ± 8.96, p = 0.021) and 12 months (13.52 ± 5.01 vs. 20.20 ± 8.67, p < 0.001). Participants with deficiency and insufficiency at baseline experienced equal benefits of supplementation in terms of anxiety and depression scores. In conclusion, in a high-risk population, a weekly vitamin D supplementation scheme was effective in alleviating anxiety and depression symptoms. More studies are needed to elucidate the relevant mechanisms.
The Reflectance Characteristics of an Inverse Moth-Eye Structure in a Silicon Substrate Depending on SF6/O2 Plasma Etching Conditions
Authors: Woo Jong-Chang , Um Doo-Seung
Published: September 2022

The global RE100 campaign is attracting attention worldwide due to climate change caused by global warming, increasingly highlighting the efficiency of renewable energy. Texturing of photovoltaic devices increases the devices’ efficiency by reducing light reflectance at their surfaces. This study introduces the change in light reflectance following the process conditions of plasma etching as a texturing process to increase the efficiency of photovoltaic cells. Isotropic etching was induced through plasma using SF6 gas, and the etch profile was modulated by adding O2 gas to reduce light reflectance. A high etch rate produces high surface roughness, which results in low surface reflectance properties. The inverse moth-eye structure was implemented using a square PR pattern arranged diagonally and showed the minimum reflectance in visible light at a tip spacing of 1 μm. This study can be applied to the development of higher-efficiency optical devices.
Micromagnetic Simulation of L10-FePt-Based Transition Jitter of Heat-Assisted Magnetic Recording at Ultrahigh Areal Density
Authors: Jongjaihan Chavakon , Kaewrawang Arkom
Published: September 2022

The areal density of hard disk drives increases every year. Increasing the areal density has limitations. Therefore, heat-assisted magnetic recording (HAMR) technology has been the candidate for increasing the areal density. At ultrahigh areal density, the main problem of the magnetic recording process is noise. Transition jitter is noise that affects the read-back signal. Hence, the performance of the magnetic recording process depends on the transition jitter. In this paper, the transition jitter of L10-FePt-based HAMR technology was simulated at the ultrahigh areal density. The micromagnetic simulation was used in the magnetic recording process. The average grain size was 5.1 nm, and the standard deviation was 0.08 nm. The recording simulation format was five tracks in a medium. It was found that a bit length of 9 nm with a track width of 16.5 nm at the areal density of 4.1 Tb/in2 had the lowest transition jitter average of 1.547 nm. In addition, the transition jitter average decreased when increasing the areal density from 4.1 to 8.9 Tb/in2. It was found that the lowest transition jitter average was 1.270 nm at an 8 nm track width and a 9 nm bit length, which achieved an ultrahigh areal density of 8.9 Tb/in2.
Nanolipogel Loaded with Tea Tree Oil for the Management of Burn: GC-MS Analysis, In Vitro and In Vivo Evaluation
Authors: Kamel Rabab , Afifi Sherif M. , Abdou Amr M. , Esatbeyoglu Tuba , AbouSamra Mona M.
Published: September 2022

The GC-MS analysis of tea tree oil (TTO) revealed 38 volatile components with sesquiterpene hydrocarbons (43.56%) and alcohols (41.03%) as major detected classes. TTO efficacy is masked by its hydrophobicity; nanoencapsulation can address this drawback. The results showed that TTO-loaded solid lipid nanoparticles (SLN1), composed of glyceryl monostearate (2% w/w) and Poloxamer188 (5% w/w), was spherical in shape with a core-shell microstructure. TTO-SLN1 showed a high entrapment efficiency (96.26 ± 2.3%), small particle size (235.0 ± 20.4 nm), low polydispersity index (0.31 ± 0.01), and high negative Zeta potential (−32 mV). Moreover, it exhibited a faster active agent release (almost complete within 4 h) compared to other formulated TTO-SLNs as well as the plain oil. TTO-SLN1 was then incorporated into cellulose nanofibers gel, isolated from sugarcane bagasse, to form the ‘TTO-loaded nanolipogel’ which had a shear-thinning behavior. Second-degree thermal injuries were induced in Wistar rats, then the burned skin areas were treated daily for 7 days with the TTO-loaded nanolipogel compared to the unmedicated nanolipogel, the TTO-loaded conventional gel, and the normal saline (control). The measurement of burn contraction proved that TTO-loaded nanolipogel exhibited a significantly accelerated skin healing, this was confirmed by histopathological examination as well as quantitative assessment of inflammatory infiltrate. This study highlighted the success of the proposed nanotechnology approach in improving the efficacy of TTO used for the repair of skin damage induced by burns.
Polypyrrole/Schiff Base Composite as Electromagnetic Absorbing Material with High and Tunable Absorption Performance
Authors: Huang Ji , Gu Huiling , Li Na , Yang Hua , Chen Gang , Zhang Lizhu , Dong Chengjun , Guan Hongtao
Published: September 2022

In recent years, Schiff base-related conjugated systems have received extensive attention, but little research has been done in the field of electromagnetic materials. In this work, an organic conjugated system based on polypyrrole/hydrazone Schiff base (PPy/HSB) composites was constructed via a Schiff base synthetic route and their electromagnetic behavior was investigated. The electromagnetic response of PPy/HSB complexes demonstrates fine electromagnetic absorption performance. When the filler loading is 30 wt% in a paraffin matrix, an absorption peak of −43.1 dB was achieved and its effective absorption bandwidth (EAB) was located in the range of 10.88−18.0 GHz. The electromagnetic response behavior of PPy/HSB complexes is explained by models involving electronic structure, multi-polarization and conductive network. The mechanisms of PPy/HSB complexes formation and HSB crystallization are also discussed through the compatibility of PPy/HSB and the structure of HSB. Moreover, the morphology transformation of HSB in the PPy/HSB systems has been studied. This study opens the exploration of organic–dielectric conjugated systems in the field of electromagnetic materials, and significantly broadens the application range of organic–dielectric–dielectric composites.
Nanohybrid of Thymol and 2D Simonkolleite Enhances Inhibition of Bacterial Growth, Biofilm Formation, and Free Radicals
Authors: Velázquez-Carriles Carlos , Macías-Rodríguez María Esther , Ramírez-Alvarado Omar , Corona-González Rosa Isela , Macías-Lamas Adriana , García-Vera Ismael , Cavazos-Garduño Adriana , Villagrán Zuamí , Silva-Jara Jorge Manuel
Published: September 2022

Due to the current concerns against opportunistic pathogens and the challenge of antimicrobial resistance worldwide, alternatives to control pathogen growth are required. In this sense, this work offers a new nanohybrid composed of zinc-layered hydroxide salt (Simonkolleite) and thymol for preventing bacterial growth. Materials were characterized with XRD diffraction, FTIR and UV–Vis spectra, SEM microscopy, and dynamic light scattering. It was confirmed that the Simonkolleite structure was obtained, and thymol was adsorbed on the hydroxide in a web-like manner, with a concentration of 0.863 mg thymol/mg of ZnLHS. Absorption kinetics was described with non-linear models, and a pseudo-second-order equation was the best fit. The antibacterial test was conducted against Escherichia coli O157:H7 and Staphylococcus aureus strains, producing inhibition halos of 21 and 24 mm, respectively, with a 10 mg/mL solution of thymol–ZnLHS. Moreover, biofilm formation of Pseudomonas aeruginosa inhibition was tested, with over 90% inhibition. Nanohybrids exhibited antioxidant activity with ABTS and DPPH evaluations, confirming the presence of the biomolecule in the inorganic matrix. These results can be used to develop a thymol protection vehicle for applications in food, pharmaceutics, odontology, or biomedical industries.
Evaluation of Different Extraction Methods on the Phenolic Profile and the Antioxidant Potential of Ceratonia siliqua L. Pods Extracts
Authors: Mansouri Fouad El , Silva Joaquim C. G. Esteves , Cacciola Francesco , Asraoui Fadoua , Tayeq Hatim , Ben Amar Yasmine Mttougui , Lovillo Miguel Palma , Chouaibi Noureddine , Brigui Jamal
Published: September 2022

The present work was designed to investigate the effects of different extraction processes, namely ultrasonic-assisted, supercritical fluid, microwave-assisted and Soxhlet applied to carob pods. The total phenolic quantification and the antioxidant activity were assessed by the means of rapid in vitro spectrophotometric assays; the phenolic profile was identified using ultra-high performance liquid chromatography coupled to mass spectrometry. The results revealed that the phenolic compounds and the antioxidant capacity varied significantly with the nature of the extraction process. The content of total phenolic compounds ranged from 11.55 to 34.38 mg GAE/g DW; the content of total flavonoids varied from 3.50 to 10.53 mg QE/g DW, and the content of condensed tannins fluctuated from 3.30 to 6.55 mg CE/ g DW. All extracts performed differently on antioxidant activity when determined by the DPPH assay producing a dose-dependent response, with IC50 extended from 11.33 to 6.07 µg/mL. HPLC analysis enabled the identification of nine compounds. As a function of the studied extraction methods, the phenolic compound contents were positively correlated with antioxidant activity.
HPLC–MS2 Analysis of Chlorophylls in Green Teas Establishes Differences among Varieties
Authors: Herrera Marta , Viera Isabel , Roca María
Published: September 2022

Green teas are nonfermented teas, the quality of which is measured by the green color. However, this category encompasses a high number of tea varieties that differ in cultivation and processing. For example, leaf or stem/bubble tea, plants cultivated under a light or shadow regime, powdered or unpowdered tea, etc. These variables determine the different qualities among green teas (Matcha, Sencha, Gyokuro, etc.) and consequently their different values on the market. Our purpose is to determine if these variables can exert an influence on the chlorophyll profile and to establish a characteristic profile for specific green teas. With such an aim, we analyzed the chlorophyll profiles of 6 different green tea varieties via HPLC-hr ESI/APCI–MS2 and identified up to 17 different chlorophyll compounds. For the first time, 132-hydroxy-chlorophylls, 132-hydroxy-pheophytins, and 151-hydroxy-lactone-pheophytins have been identified in green teas. Shadow teas (Matcha and Sencha) and light-regimen green teas can be statistically differentiated by the total chlorophyll content and the a/b ratio. However, only Matcha tea contains a higher proportion of chlorophylls a and b among the green tea varieties analyzed, justifying the higher quality and price of this variety. Other chlorophyll metabolites (pheophytins, pyropheophytins, and oxidized chlorophylls) are indicative of the various processing and storage conditions.
Direct In Vitro Comparison of the Anti-Leishmanial Activity of Different Olive Oil Total Polyphenolic Fractions and Assessment of Their Combined Effects with Miltefosine
Authors: Gogou Georgia , Koutsoni Olga S. , Stathopoulos Panagiotis , Skaltsounis Leandros A. , Halabalaki Maria , Dotsika Eleni
Published: September 2022

The bioactive compounds present in the edible products of the olive tree have been extensively studied and their favorable effects on various disease risk factors have been demonstrated. The aim of this study was to perform a comparative analysis of the anti-leishmanial effects of total phenolic fractions (TPFs) derived from extra virgin olive oil with different phenolic contents and diverse quantitative patterns. Moreover, the present study investigated their association with miltefosine, a standard anti-leishmanial drug, against both extracellular promastigotes and intracellular amastigotes of a viscerotropic and a dermotropic Leishmania strain. The chemical compositions of TPFs were determined by high performance liquid chromatography with diode array detection (HPLC-DAD). Analysis of parasite growth kinetics, reactive oxygen species production and apoptotic events were determined by microscopy and flow cytometry. Our results revealed that the presence of oleacein (OLEA) and oleocanthal (OLEO) secoiridoids enhances the anti-leishmanial effect of TPF. The association between TPFs and miltefosine was suggested as being additive in Leishmania infantum and Leishmania major promastigotes, and as antagonistic in intracellular amastigotes, as was evaluated with the modified isobologram method. The obtained data verified that TPFs are bioactive dietary extracts with a strong anti-leishmanial activity and highlighted that fractions that are richer in OLEA and OLEO phenolic compounds possess stronger inhibitory effects against parasites. This study may contribute to improving the therapeutic approaches against leishmaniasis.
Comparison of Different Volatile Extraction Methods for the Identification of Fishy Off-Odor in Fish By-Products
Authors: Zhang Yuanyuan , Tang Long , Zhang Yu , Song Huanlu , Raza Ali , Pan Wenqing , Gong Lin , Jiang Can
Published: September 2022

This study was conducted to analyze volatile odor compounds and key odor-active compounds in the fish soup using fish scarp and bone. Five extraction methods, including solid-phase microextraction (SPME), dynamic headspace sampling (DHS), solvent-assisted flavor evaporation (SAFE), stir bar sorptive extraction (SBSE), liquid-liquid extraction (LLE), were compared and SPME was finally selected as the best extraction method for further study. The volatile odor compounds were analyzed by gas chromatography-olfactometry-mass spectrometry (GC-O-MS) and comprehensive two-dimensional gas chromatography-olfactometry-mass spectrometry (GC × GC-O-MS) techniques, and the key odor-active compounds were identified via aroma extract dilution analysis (AEDA) and relative odor activity value (r-OAV) calculation. A total of 38 volatile compounds were identified by GC-O-MS, among which 10 were declared as odor-active compounds. Whereas 39 volatile compounds were identified by GC × GC-O-MS, among which 12 were declared as odor-active compounds. The study results revealed that 1-octen-3-one, 2-pentylfuran, (E)-2-octenal, 1-octen-3-one, hexanal, 1-octen-3-ol, 6-methylhept-5-en-2-one, (E,Z)-2,6-nondienal and 2-ethyl-3,5-dimethylpyrazine were the key odor-active compounds in the fish soup.
Towards the Growth of Hexagonal Boron Nitride on Ge(001)/Si Substrates by Chemical Vapor Deposition
Authors: Franck Max , Dabrowski Jaroslaw , Schubert Markus Andreas , Wenger Christian , Lukosius Mindaugas
Published: September 2022

The growth of hexagonal boron nitride (hBN) on epitaxial Ge(001)/Si substrates via high-vacuum chemical vapor deposition from borazine is investigated for the first time in a systematic manner. The influences of the process pressure and growth temperature in the range of 10−7–10−3 mbar and 900–980 °C, respectively, are evaluated with respect to morphology, growth rate, and crystalline quality of the hBN films. At 900 °C, nanocrystalline hBN films with a lateral crystallite size of ~2–3 nm are obtained and confirmed by high-resolution transmission electron microscopy images. X-ray photoelectron spectroscopy confirms an atomic N:B ratio of 1 ± 0.1. A three-dimensional growth mode is observed by atomic force microscopy. Increasing the process pressure in the reactor mainly affects the growth rate, with only slight effects on crystalline quality and none on the principle growth mode. Growth of hBN at 980 °C increases the average crystallite size and leads to the formation of 3–10 well-oriented, vertically stacked layers of hBN on the Ge surface. Exploratory ab initio density functional theory simulations indicate that hBN edges are saturated by hydrogen, and it is proposed that partial de-saturation by H radicals produced on hot parts of the set-up is responsible for the growth.
Conductometric ppb-Level CO Sensors Based on In2O3 Nanofibers Co-Modified with Au and Pd Species
Authors: Han Wenjiang , Yang Jiaqi , Jiang Bin , Wang Xi , Wang Chong , Guo Lanlan , Sun Yanfeng , Liu Fangmeng , Sun Peng , Lu Geyu
Published: September 2022

Carbon monoxide (CO) is one of the most toxic gases to human life. Therefore, the effective monitoring of it down to ppb level is of great significance. Herein, a series of In2O3 nanofibers modified with Au or Pd species or simultaneous Au and Pd species have been prepared by electrospinning combined with a calcination process. The as-obtained samples are applied for the detection of CO. Gas-sensing investigations indicate that 2 at% Au and 2 at% Pd-co-modified In2O3 nanofibers exhibit the highest response (21.7) to 100 ppm CO at 180 °C, and the response value is ~8.5 times higher than that of pure In2O3 nanofibers. More importantly, the detection limit to CO is about 200 ppb with a response value of 1.23, and is obviously lower than that (6 ppm) of pure In2O3 nanofibers. In addition, the sensor also shows good stability within 19 days. These demonstrate that co-modifying In2O3 nanofibers with suitable amounts of Pd and Au species might be a meaningful strategy for the development of high-performance carbon monoxide gas sensors.
Tapping-Actuated Triboelectric Nanogenerator with Surface Charge Density Optimization for Human Motion Energy Harvesting
Authors: Duque Marcos , Murillo Gonzalo
Published: September 2022

In this article, triboelectric effect has been used to harvest mechanical energy from human motion and convert it into electrical energy. To do so, different ways of optimizing the energy generated have been studied through the correct selection of materials, the design of new spacers to improve the contact surface area, and charge injection by high-voltage corona charging to increase the charge density of dielectric materials. Finally, a triboelectric nanogenerator (TENG) has been manufactured, which is capable of collecting the mechanical energy of the force applied by hand tapping and using it to power miniaturized electronic sensors in a self-sufficient and sustainable way. This work shows the theoretical concept and simulations of the proposed TENG device, as well as the experimental work carried out.
Distortions in Spatial Mental Representation Affect Sketch Maps in Young Adults
Authors: Lopez Antonella , Bosco Andrea
Published: September 2022

Humans tend to misrepresent spatial information which leads to systematic errors due to distorted organizational processes regarding metric and positional judgments. This study is aimed at analyzing metric and positional distortions in cognitive maps by using external representations, namely sketch maps, in two experiments with young participants. In the first experiment, we use the sketching area of Northern Europe. In the second experiment, the University campus area was used. The first aim was to test the hypothesis that the presence of the sea between the triplet of landmarks generates an overestimation of the distances between them in the case of Northern Europe; and to test the hypothesis that the number of turns in a route influences the overestimation of the distance between landmarks in the case of the campus area. The second aim was to investigate alignment and rotation errors using the same maps. Concerning metric errors, the results showed the overestimation of distances with a geographical gap between the cities (the sea in the Northern European Area), and those with more turns between landmarks (the campus area). The results concerning rotations and alignments were in line with the previous research about positional errors. The present study extended findings on distortions in spatial mental representation as emerging from verbal judgments, to sketch maps: direct visuospatial external representations eluding the conversion into verbal coding of spatial information. The presence of distortions in cognitive maps could be considered the consequence of the encoding of spatial information.
Situating the Father: Strengthening Interdisciplinary Collaborations between Sociology, History and the Emerging POHaD Paradigm
Authors: Mayes Christopher , Lawson-Boyd Elsher , Meloni Maurizio
Published: September 2022

(1) Background: Albeit the main focus remains largely on mothers, in recent years Developmental Origins of Health and Disease (DOHaD) scientists, including epigeneticists, have started to examine how a father’s environment affects disease risk in children and argued that more attention needs to be given to father’s health-related behaviors for their influence on offspring at preconception (i.e., sperm health) as well as paternal lifestyle influences over the first 1000 days. This research ushers in a new paternal origins of health and disease (POHaD) paradigm and is considered a welcome equalization to the overemphasis on maternal influences. Epigeneticists are excited by the possibilities of the POHaD paradigm but are also cautious about how to interpret data and avoid biased impression of socio-biological reality. (2) Methods: We review sociological and historical literatures on the intersection of gender, food and diet across different social and historical contexts to enrich our understanding of the father; (3) Results: Sociological and historical research on family food practices and diet show that there are no “fathers” in the abstract or vacuum, but they are differently classed, racialized and exist in socially stratified situations where choices may be constrained or unavailable. This confirms that epigeneticists researching POHaD need to be cautious in interpreting paternal and maternal dietary influences on offspring health; (4) Conclusions: We suggest that interdisciplinary approach to this new paradigm, which draws on sociology, history and public health, can help provide the social and historical context for interpreting and critically understanding paternal lifestyles and influences on offspring health.
Thyroidal and Extrathyroidal Requirements for Iodine and Selenium: A Combined Evolutionary and (Patho)Physiological Approach
Authors: Dijck-Brouwer D. A. Janneke , Muskiet Frits A. J. , Verheesen Richard H. , Schaafsma Gertjan , Schaafsma Anne , Geurts Jan M. W.
Published: September 2022

Iodide is an antioxidant, oxidant and thyroid hormone constituent. Selenoproteins are needed for triiodothyronine synthesis, its deactivation and iodine release. They also protect thyroidal and extrathyroidal tissues from hydrogen peroxide used in the ‘peroxidase partner system’. This system produces thyroid hormone and reactive iodine in exocrine glands to kill microbes. Exocrine glands recycle iodine and with high urinary clearance require constant dietary supply, unlike the thyroid. Disbalanced iodine-selenium explains relations between thyroid autoimmune disease (TAD) and cancer of thyroid and exocrine organs, notably stomach, breast, and prostate. Seafood is iodine unconstrained, but selenium constrained. Terrestrial food contains little iodine while selenium ranges from highly deficient to highly toxic. Iodine vs. TAD is U-shaped, but only low selenium relates to TAD. Oxidative stress from low selenium, and infection from disbalanced iodine-selenium, may generate cancer of thyroid and exocrine glands. Traditional Japanese diet resembles our ancient seashore-based diet and relates to aforementioned diseases. Adequate iodine might be in the milligram range but is toxic at low selenium. Optimal selenoprotein-P at 105 µg selenium/day agrees with Japanese intakes. Selenium upper limit may remain at 300–400 µg/day. Seafood combines iodine, selenium and other critical nutrients. It brings us back to the seashore diet that made us what we currently still are.
Amino Acid Profiles and Nutritional Evaluation of Fresh Sweet–Waxy Corn from Three Different Regions of China
Authors: Li Ziqi , Hong Tiannuo , Shen Guohui , Gu Yanting , Guo Yanzhi , Han Juan
Published: September 2022

This study conducted a comparative analysis of the amino acid compositions of Chinese Huangnuo 9 fresh sweet–waxy corn from three different provinces in China—Inner Mongolia, Jilin, and Heilongjiang Province. Moreover, we established a nutritive evaluation system based on amino acid profiles to evaluate, compare, and rank the fresh sweet–waxy corn planted in different regions. A total of 17 amino acids were quantified, and the amino acid composition of fresh sweet–waxy corn was analyzed and evaluated. The amino acid quality was determined by the amino acid pattern spectrum, chemical evaluations (including CS, AAS, EAAI, BV, U(a,u), NI, F, predict PER, and PDCAAS), flavor evaluation, amino acid matching degree evaluation, and the results of the factor analysis. The results showed that the protein content of fresh corn 1–1 from Inner Mongolia was the highest (40.26 ± 0.35 mg/g), but the factor analysis results, digestion, and absorption efficiency of fresh corn 1–2 were the best. The amino acid profile of fresh corn 1–1 was closest to each evaluation’s model spectrum. The results of the diversity evaluations in fresh corn 3–2 were the best, and fresh corn 3–3 had the most essential amino acid content. A total of 17 amino acids in fresh corn were divided into three principal component factor analyses: functional principal components (Leu, Pro, Glu, His, Ile, Ser, Met, Val, Tyr, Thr), regulatory principal components (Lys, Gly, Ala, Asp, Arg, Trp), and protection principal components (Phe). The scores of the three principal components and the comprehensive score in fresh corn 1–2 were all the highest, followed by 3–3 and 1–1. The amino acid nutritional values of fresh corn 1–2 were the highest in 12 samples.
Emerging Role of Nicotinamide Riboside in Health and Diseases
Authors: Sharma Chiranjeev , Donu Dickson , Cen Yana
Published: September 2022

Among all the NAD+ precursors, nicotinamide riboside (NR) has gained the most attention as a potent NAD+-enhancement agent. This recently discovered vitamin, B3, has demonstrated excellent safety and efficacy profiles and is orally bioavailable in humans. Boosting intracellular NAD+ concentrations using NR has been shown to provide protective effects against a broad spectrum of pathological conditions, such as neurodegenerative diseases, diabetes, and hearing loss. In this review, an integrated overview of NR research will be presented. The role NR plays in the NAD+ biosynthetic pathway will be introduced, followed by a discussion on the synthesis of NR using chemical and enzymatic approaches. NR’s effects on regulating normal physiology and pathophysiology will also be presented, focusing on the studies published in the last five years.