bluuebunny/crossref_metadata_2025 huggingface dataset:
Artemisia annua L. plants lacking Bornyl diPhosphate Synthase reallocate carbon from monoterpenes to sesquiterpenes except artemisinin
Authors: Czechowski Tomasz , Branigan Caroline , Rae Anne , Rathbone Deborah , Larson Tony R. , Harvey David , Catania Theresa M. , Zhang Dong , Li Yi , Salmon Melissa , Bowles Dianna J. , O´Maille Paul , Graham Ian A.
Published: October 2022

The monoterpene camphor is produced in glandular secretory trichomes of the medicinal plant Artemisia annua, which also produces the antimalarial drug artemisinin. We have found that, depending on growth conditions, camphor can accumulate at levels ranging from 1- 10% leaf dry weight (LDW) in the Artemis F1 hybrid, which has been developed for commercial production of artemisinin at up to 1% LDW. We discovered that a camphor null (camphor-0) phenotype segregates in the progeny of self-pollinated Artemis material. Camphor-0 plants also show reduced levels of other less abundant monoterpenes and increased levels of the sesquiterpene precursor farnesyl pyrophosphate plus sesquiterpenes, including enzymatically derived artemisinin pathway intermediates but not artemisinin. One possible explanation for this is that high camphor concentrations in the glandular secretory trichomes play an important role in generating the hydrophobic conditions required for the non-enzymatic conversion of dihydroartemisinic acid tertiary hydroperoxide to artemisinin. We established that the camphor-0 phenotype associates with a genomic deletion that results in loss of a Bornyl diPhosphate Synthase (AaBPS) gene candidate. Functional characterization of the corresponding enzyme in vitro confirmed it can catalyze the first committed step in not only camphor biosynthesis but also in a number of other monoterpenes, accounting for over 60% of total volatiles in A. annua leaves. This in vitro analysis is consistent with loss of monoterpenes in camphor-0 plants. The AaBPS promoter drives high reporter gene expression in A. annua glandular secretory trichomes of juvenile leaves with expression shifting to non-glandular trichomes in mature leaves, which is consistent with AaBPS transcript abundance.
Efficient attention-based CNN network (EANet) for multi-class maize crop disease classification
Authors: Albahli Saleh , Masood Momina
Published: October 2022

Maize leaf disease significantly reduces the quality and overall crop yield. Therefore, it is crucial to monitor and diagnose illnesses during the growth season to take necessary actions. However, accurate identification is challenging to achieve as the existing automated methods are computationally complex or perform well on images with a simple background. Whereas, the realistic field conditions include a lot of background noise that makes this task difficult. In this study, we presented an end-to-end learning CNN architecture, Efficient Attention Network (EANet) based on the EfficientNetv2 model to identify multi-class maize crop diseases. To further enhance the capacity of the feature representation, we introduced a spatial-channel attention mechanism to focus on affected locations and help the detection network accurately recognize multiple diseases. We trained the EANet model using focal loss to overcome class-imbalanced data issues and transfer learning to enhance network generalization. We evaluated the presented approach on the publically available datasets having samples captured under various challenging environmental conditions such as varying background, non-uniform light, and chrominance variances. Our approach showed an overall accuracy of 99.89% for the categorization of various maize crop diseases. The experimental and visual findings reveal that our model shows improved performance compared to conventional CNNs, and the attention mechanism properly accentuates the disease-relevant information by ignoring the background noise.
Drought effects on tree growth, water use efficiency, vulnerability and canopy health of Quercus variabilis-Robinia pseudoacacia mixed plantation
Authors: Jia HanSen , Guan ChongFan , Zhang JinSong , He ChunXia , Yin ChangJun , Meng Ping
Published: October 2022

Drought-induced forest canopy die-back and tree mortality have been commonly recorded in the lithoid mountainous regions of northern China. However, the capacity of trees to regulate their carbon and water balance in response to drought remains inadequately understood. We measured tree growth, intrinsic water use efficiency (iWUE), vulnerability, and canopy health during drought events using dendrochronology, C isotope measurements, and a tree canopy health survey in a mixed plantation of Quercus variabilis and Robinia pseudoacacia. Resistance (Rt), recovery (Rc), resilience (Rs), and increased amplitude in iWUE compared to the indices 3 years before drought (iWUEr) were calculated for each species across the dominant tree (D), co-dominant tree (CD), and suppressed tree (S). Our results revealed that D and CD showed lower Rt, higher Rc, and higher iWUEr than S. After exposure to multiple sequential drought events, Q. variabilis showed an increasing trend in Rt, and R. pseudoacacia showed a decreasing trend in Rc. R. pseudoacacia exhibited a more conservative strategy towards drought, resulting in a negative SRt-iWUEr (slope of the linear model fitted to capture the trend between Rt and iWUEr) during drought events than Q. variabilis. For individual trees, lower Rc or positive SRt-iWUErQ. variabilis and negative SRt-iWUErR. pseudoacacia were more susceptible to canopy die-back. In conclusion, our study offers a new perspective for improved management practices in the design of silvicultural actions for forestry plantations in lithoid mountainous areas with increasing drought risk.
Evaluation of Rosa germplasm resources and analysis of floral fragrance components in R. rugosa
Authors: Cheng Xi , Feng Yan , Chen Dongliang , Luo Chang , Yu Xiaofang , Huang Conglin
Published: October 2022

Rosa rugosa (Rosaceae) is an important functional plant used in food products, tea, and aromatherapy. Characteristics of R. rugosa varieties based on the biological traits and floral fragrant components were studied by applying an analytic hierarchy process, headspace solid-phase microextraction gas chromatography–mass spectrometry, and metabolomic analysis. The 77 Rosa accessions (comprising 27 R. rugosa varieties, 43 scented R. hybrida cultivars, and seven fragrant R. species) were grouped into nine classes based on 17 morphological characters and 16 targeted fragrant substances by cluster analysis. Three R. rugosa cultivars differing in fragrance type were selected for volatile metabolomics analysis at four stages of flower development. In total, 156 differential volatile organic compounds (VOC) were detected and the VOC content patterns were further investigated in two important metabolic pathways (the monoterpenoid biosynthetic pathway, and the phenylalanine, tyrosine, and tryptophan biosynthesis pathway). The results provide a foundation for efficient use of Rosa germplasm and insights into the utilization of R. rugosa as a functional flower.
The roles of HD-ZIP proteins in plant abiotic stress tolerance
Authors: Li Yuxia , Yang Zongran , Zhang Yuanyuan , Guo Jinjiao , Liu Lili , Wang Chengfeng , Wang Baoshan , Han Guoliang
Published: October 2022

Homeodomain leucine zipper (HD-ZIP) proteins are plant-specific transcription factors that contain a homeodomain (HD) and a leucine zipper (LZ) domain. The highly conserved HD binds specifically to DNA and the LZ mediates homodimer or heterodimer formation. HD-ZIP transcription factors control plant growth, development, and responses to abiotic stress by regulating downstream target genes and hormone regulatory pathways. HD-ZIP proteins are divided into four subclasses (I–IV) according to their sequence conservation and function. The genome-wide identification and expression profile analysis of HD-ZIP proteins in model plants such as Arabidopsis (Arabidopsis thaliana) and rice (Oryza sativa) have improved our understanding of the functions of the different subclasses. In this review, we mainly summarize and discuss the roles of HD-ZIP proteins in plant response to abiotic stresses such as drought, salinity, low temperature, and harmful metals. HD-ZIP proteins mainly mediate plant stress tolerance by regulating the expression of downstream stress-related genes through abscisic acid (ABA) mediated signaling pathways, and also by regulating plant growth and development. This review provides a basis for understanding the roles of HD-ZIP proteins and potential targets for breeding abiotic stress tolerance in plants.
Genomic footprints of selection in early-and late-flowering pearl millet landraces
Authors: Faye Adama , Barnaud Adeline , Kane Ndjido Ardo , Cubry Philippe , Mariac Cédric , Burgarella Concetta , Rhoné Bénédicte , Faye Aliou , Olodo Katina Floride , Cisse Aby , Couderc Marie , Dequincey Anaïs , Zekraouï Leïla , Moussa Djibo , Tidjani Moussa , Vigouroux Yves , Berthouly-Salazar Cécile
Published: October 2022

Pearl millet is among the top three-cereal production in one of the most climate vulnerable regions, sub-Saharan Africa. Its Sahelian origin makes it adapted to grow in poor sandy soils under low soil water regimes. Pearl millet is thus considered today as one of the most interesting crops to face the global warming. Flowering time, a trait highly correlated with latitude, is one of the key traits that could be modulated to face future global changes. West African pearl millet landraces, can be grouped into early- (EF) and late-flowering (LF) varieties, each flowering group playing a specific role in the functioning and resilience of Sahelian smallholders. The aim of this study was thus to detect genes linked to flowering but also linked to relevant traits within each flowering group. We thus investigated genomic and phenotypic diversity in 109 pearl millet landrace accessions, i.e., 66 early-flowering and 43 late-flowering, grown in the groundnut basin, the first area of rainfed agriculture in Senegal dominated by dry cereals (millet, maize, and sorghum) and legumes (groundnuts, cowpeas). We were able to confirm the role of PhyC gene in pearl millet flowering and identify several other genes that appear to be as much as important, such as FSR12 and HAC1. HAC1 and two other genes appear to be part of QTLs previously identified and deserve further investigation. At the same time, we were able to highlight a several genes and variants that could contribute to the improvement of pearl millet yield, especially since their impact was demonstrated across flowering cycles.
Precision phenotyping across the life cycle to validate and decipher drought-adaptive QTLs of wild emmer wheat (Triticum turgidum ssp. dicoccoides) introduced into elite wheat varieties
Authors: Lauterberg Madita , Saranga Yehoshua , Deblieck Mathieu , Klukas Christian , Krugman Tamar , Perovic Dragan , Ordon Frank , Graner Andreas , Neumann Kerstin
Published: October 2022

Drought events or the combination of drought and heat conditions are expected to become more frequent due to global warming, and wheat yields may fall below their long-term average. One way to increase climate-resilience of modern high-yielding varieties is by their genetic improvement with beneficial alleles from crop wild relatives. In the present study, the effect of two beneficial QTLs introgressed from wild emmer wheat and incorporated in the three wheat varieties BarNir, Zahir and Uzan was studied under well-watered conditions and under drought stress using non-destructive High-throughput Phenotyping (HTP) throughout the life cycle in a single pot-experiment. Plants were daily imaged with RGB top and side view cameras and watered automatically. Further, at two time points, the quantum yield of photosystem II was measured with a top view FluorCam. The QTL carrying near isogenic lines (NILs) were compared with their corresponding parents by t-test for all non-invasively obtained traits and for the manually determined agronomic and yield parameters. Data quality of phenotypic traits (repeatability) in the controlled HTP experiment was above 85% throughout the life cycle and at maturity. Drought stress had a strong effect on growth in all wheat genotypes causing biomass reduction from 2% up to 70% at early and late points in the drought period, respectively. At maturity, the drought caused 47–55% decreases in yield-related traits grain weight, straw weight and total biomass and reduced TKW by 10%, while water use efficiency (WUE) increased under drought by 29%. The yield-enhancing effect of the introgressed QTLs under drought conditions that were previously demonstrated under field/screenhouse conditions in Israel, could be mostly confirmed in a greenhouse pot experiment using HTP. Daily precision phenotyping enabled to decipher the mode of action of the QTLs in the different genetic backgrounds throughout the entire wheat life cycle. Daily phenotyping allowed a precise determination of the timing and size of the QTLs effect (s) and further yielded information about which image-derived traits are informative at which developmental stage of wheat during the entire life cycle. Maximum height and estimated biovolume were reached about a week after heading, so experiments that only aim at exploring these traits would not need a longer observation period. To obtain information on different onset and progress of senescence, the CVa curves represented best the ongoing senescence of plants. The QTL on 7A in the BarNir background was found to improve yield under drought by increased biomass growth, a higher photosynthetic performance, a higher WUE and a “stay green effect.”
Non-coding RNAs fine-tune the balance between plant growth and abiotic stress tolerance
Authors: Zhang Yingying , Zhou Ye , Zhu Weimin , Liu Junzhong , Cheng Fang
Published: October 2022

To survive in adverse environmental conditions, plants have evolved sophisticated genetic and epigenetic regulatory mechanisms to balance their growth and abiotic stress tolerance. An increasing number of non-coding RNAs (ncRNAs), including small RNAs (sRNAs) and long non-coding RNAs (lncRNAs) have been identified as essential regulators which enable plants to coordinate multiple aspects of growth and responses to environmental stresses through modulating the expression of target genes at both the transcriptional and posttranscriptional levels. In this review, we summarize recent advances in understanding ncRNAs-mediated prioritization towards plant growth or tolerance to abiotic stresses, especially to cold, heat, drought and salt stresses. We highlight the diverse roles of evolutionally conserved microRNAs (miRNAs) and small interfering RNAs (siRNAs), and the underlying phytohormone-based signaling crosstalk in regulating the balance between plant growth and abiotic stress tolerance. We also review current discoveries regarding the potential roles of ncRNAs in stress memory in plants, which offer their descendants the potential for better fitness. Future ncRNAs-based breeding strategies are proposed to optimize the balance between growth and stress tolerance to maximize crop yield under the changing climate.
Screening of protonstatin-1 (PS-1) analogs for improved inhibitors of plant plasma membrane H+-ATPase activity
Authors: Yang Yongqing , Liu Xiaohui , Wang Xin , Lv Wanjia , Liu Xiao , Ma Liang , Fu Haiqi , Song Shu , Lei Xiaoguang
Published: October 2022

We previously identified protonstatin-1 (PS-1) as a selective inhibitor of plasma membrane H+-ATPase (PM H+-ATPase) activity and used it as a tool to validate the chemiosmotic model for polar auxin transport. Here, to obtain compounds with higher affinity than PS-1 for PM H+-ATPase, we synthesized 34 PS-1 analogs and examined their ability to inhibit PM H+-ATPase activity. The 34 analogs showed varying inhibitory effects on the activity of this enzyme. The strongest effect was observed for the small molecule PS-2, which was approximately five times stronger than PS-1. Compared to PS-1, PS-2 was also a stronger inhibitor of auxin uptake as well as acropetal and basipetal polar auxin transport in Arabidopsis thaliana seedlings. Because PS-2 is a more potent inhibitor of PM H+-ATPase than PS-1, we believe that this compound could be used as a tool to study the functions of this key plant enzyme.
Integrated transcriptomics and miRNAomics provide insights into the complex multi-tiered regulatory networks associated with coleoptile senescence in rice
Authors: Sasi Jyothish Madambikattil , VijayaKumar Cheeni , Kukreja Bharti , Budhwar Roli , Shukla Rohit Nandan , Agarwal Manu , Katiyar-Agarwal Surekha
Published: October 2022

Coleoptile is the small conical, short-lived, sheath-like organ that safeguards the first leaf and shoot apex in cereals. It is also the first leaf-like organ to senesce that provides nutrition to the developing shoot and is, therefore, believed to play a crucial role in seedling establishment in rice and other grasses. Though histochemical studies have helped in understanding the pattern of cell death in senescing rice coleoptiles, genome-wide expression changes during coleoptile senescence have not yet been explored. With an aim to investigate the gene regulation underlying the coleoptile senescence (CS), we performed a combinatorial whole genome expression analysis by sequencing transcriptome and miRNAome of senescing coleoptiles. Transcriptome analysis revealed extensive reprogramming of 3439 genes belonging to several categories, the most prominent of which encoded for transporters, transcription factors (TFs), signaling components, cell wall organization enzymes, redox homeostasis, stress response and hormone metabolism. Small RNA sequencing identified 41 known and 21 novel miRNAs that were differentially expressed during CS. Comparison of gene expression and miRNA profiles generated for CS with publicly available leaf senescence (LS) datasets revealed that the two aging programs are remarkably distinct at molecular level in rice. Integration of expression data of transcriptome and miRNAome identified high confidence 140 miRNA-mRNA pairs forming 42 modules, thereby demonstrating multi-tiered regulation of CS. The present study has generated a comprehensive resource of the molecular networks that enrich our understanding of the fundamental pathways regulating coleoptile senescence in rice.
PEG-induced physiological drought for screening winter wheat genotypes sensitivity – integrated biochemical and chlorophyll a fluorescence analysis
Authors: Peršić Vesna , Ament Anita , Antunović Dunić Jasenka , Drezner Georg , Cesar Vera
Published: October 2022

This study aimed to screen different winter wheat genotypes at the onset of metabolic changes induced by water deficit to comprehend possible adaptive features of photosynthetic apparatus function and structure to physiological drought. The drought treatment was the most influential variable affecting plant growth and relative water content, and genotype variability determined with what intensity varieties of winter wheat seedlings responded to water deficit. PEG-induced drought, as expected, changed phenomenological energy fluxes and the efficiency with which an electron is transferred to final PSI acceptors. Based on the effect size, fluorescence parameters were grouped to represent photochemical parameters, that is, the donor and acceptor side of PSII (PC1); the thermal phase of the photosynthetic process, or the electron flow around PSI, and the chain of electrons between PSII and PSI (PC2); and phenomenological energy fluxes per cross-section (PC3). Furthermore, four distinct clusters of genotypes were discerned based on their response to imposed physiological drought, and integrated analysis enabled an explanation of their reactions’ specificity. The most reliable JIP-test parameters for detecting and comparing the drought impact among tested genotypes were the variable fluorescence at K, L, I step, and PITOT. To conclude, developing and improving screening methods for identifying and evaluating functional relationships of relevant characteristics that are useful for acclimation, acclimatization, and adaptation to different types of drought stress can contribute to the progress in breeding research of winter wheat drought-tolerant lines.
High-quality development of virtual reality industry from the perspective of environmental regulation
Authors: Zhang Liping , Yang Yi , Xiong Kaiqi
Published: October 2022

From the perspective of environmental regulation, this paper constructed a high-quality development evaluation system for the virtual reality industry (hereinafter referred to as VRI), calculated the high-quality development level of the VRI, and empirically studied the path of high-quality development of VRI based on the research methods of entropy weight method and structural equation model (hereinafter referred to as SEM), as well as the data of listed companies of VRI in China from 2011 to 2020. The results showed that (1) VRI adapted to the high-quality development level, but there was a certain gap, its high-quality development degree shows a rising trend; (2) the degree of corporate public welfare development had a positive effect on corporate profitability, while the degree of green development and coordinated development had a weak impact on corporate profitability; (3) open and innovative dimensions had a reverse effect on the profitability of enterprises. Enterprises should seek ways to reduce environmental costs and enhance competitiveness. The industrial chain, technology chain and value chain should be constructed to promote the healthy development of VRI and provide support for the formulation of high-quality development policies for VRI.
The effectiveness of internal cohesion psychotherapy in treating young clients with depression and anxiety disorders: The role of developmental assets in Kosovo context
Authors: Uka Fitim , Gashi Shkumbin , Gashi Arlinda , Gllogu Diellza , Musliu Arian , Krasniqi Albina , Statovci Albina , Sopjani Vanesa , Perçuku Veronë , Sadikovic Irma , Wiium Nora
Published: October 2022

The positive youth development approach (PYD) is widely used as a meaningful framework to guide research, policy, and intervention, to support young people to develop their full potential. Psychotherapy, on the other hand, is a verbal and psychological procedure that can be a suitable solution to mental health concerns, which are prevalent among youth. This study aimed to explore the potential role of developmental assets in treating clients with depression and anxiety disorders using Internal Cohesion Psychotherapy (ICP). In total, 10 young people who took at least five sessions of ICP were part of this study. In-depth semi-structured interviews were conducted to gather information about clients’ experiences with ICP and their perception/opinions on the presence of developmental assets in their lives. The results confirm the effectiveness of ICP in treating depression and anxiety, while clients acknowledge the role of developmental assets in their psychotherapeutic process. The current study has important theoretical, practical, and research implications. It provides evidence on how clients use their developmental assets in maximizing the effectiveness of the ICP process. The usage of developmental assets to enhance the effectiveness of psychotherapy opens a new path for further research and can serve as a foundation ground for intervention on mental health among youth.
Questionnaire development on measuring parents’ anxiety about their children’s education: Empirical evidence of parental perceived anxiety data for primary and secondary school students in China
Authors: Sun Jiangjie , Liu Tong , Gao Yufei , Li Hui , Chen Yao , Diao Haibao , Zhang Genfa , Shen Hui , Chang Rong , Yu Zhenliang , Lu Jingru , Liang Liang , Zhang Liping
Published: October 2022

Background

With the implementation of the “double reduction” policy in China, parents of primary and secondary school students are experiencing a growing trend of educational anxiety that needs to be alleviated.

Objective

To manage the education anxiety risk of parents of primary and secondary school students, a measurement questionnaire of parents’ anxiety about their children’s education (MQPAE) was developed and its reliability and validity were evaluated.

Methods

A self-administered MQPAE was developed. An online crowdsourcing questionnaire platform was used to collect data on parents’ anxiety about their children’s education (PAE), and parents of primary and secondary school students in Hefei, China, were selected as the study population. The randomly extracted 5,747 questionnaires were gradually screened by discrete trend method, t-test, and Pearson’s correlation coefficient method for the initial screening of PAE items, based on which exploratory factor analysis (EFA) was conducted for the final screening of questionnaire items and the reliability of the questionnaire. The reliability of the questionnaire was assessed by internal consistency and Pearson’s correlation coefficient analysis. Confirmatory factor analysis (CFA) was conducted using 639 pre-selected data to investigate the validity of the questionnaire. Structural equation modeling was used to investigate the structural validity of the questionnaire, and average variance extracted (AVE), combined reliability (CR), and maximum of shared squared variance (MSV) were used to test for convergent and discriminant validity.

Results

Exploratory factor analysis extracted five factors with a cumulative variance contribution of 65.66%. The CFA showed that χ2/df = 4.306, CFI = 0.920, NFI = 0.898, RMSEA = 0.072<0.08, AGFI = 0.839>0.80, PNFI = 0.793 and PGFI = 0.708. The overall Cronbach’s α coefficient of the questionnaire was 0.956, and the factors’ Cronbach’s α coefficients were 0.926, 0.857, 0.913, 0.901, and 0.768, respectively. Repeated measurements of Pearson’s correlation coefficients were 0.908, 0.911, 0.873, 0.891, 0.907 and 0.885 (all p < 0.001). The AVE was greater than 0.5 and the CR was greater than 0.7, and the value of the MSV was less than the corresponding AVE.

Conclusion

The MQPAE has good reliability and validity and can be used in studies related to PAE of primary and secondary school students.
Generation Y medical doctors’ experiences of a positive psychology 2.0 intervention for burnout in a South African public hospital
Authors: Oosthuizen Rudolf M. , Disemelo Keitumetse , Mayer Claude-Hélène
Published: October 2022

The aim of the research was to explore experiences of a Positive Psychology 2.0 (PP2.0) intervention for burnout among Generation Y medical doctors working in a South African public hospital. The emphasis was on the potential benefits and recommended intervention amendments in a specific cultural context of South Africa. A phenomenological approach was followed in the collective case study. The Maslach Burnout Inventory was administered in phases I and III to quantify the level of burnout. This study offers findings that could be incorporated into a bigger burnout intervention strategy in the South African public hospital, involving all stakeholders to ensure that burnout is combatted on a long-term basis. Furthermore, the findings emphasized certain culture-specific structural issues and the impact that the neglection of burnout has on newly qualified medical doctors working in a South African public hospital and patients in their care. Certain recommendations were made for the South African public hospital for future research in PP2.0 interventions and for facilitators working with burnout among newly qualified medical doctors.
Moderating Role of Initiative on the Relationship Between Intrinsic Motivation, and Self-Efficacy on Entrepreneurial Intention
Authors: Ogba Francisca N. , Ogba Kalu T. U. , Ugwu Lawrence E. , Emma-Echiegu Nkechi , Eze Adaobi , Agu Solomon Amaechi , Aneke Bernard Akonam
Published: October 2022

The growing population of new graduates and the increasing scarcity of employment opportunities have made entrepreneurship an unavoidable option for employment and self-sustenance. This study investigates the effect of the initiative in moderating the relationship between intrinsic motivation, self-efficacy, and entrepreneurship intention through the integrated framework of theory of planned behaviour, self-determination, and humanism. This study contributes insights to how these factors moderated by initiative influence entrepreneurial intention among graduating students of tertiary institutions in Nigeria. This study adopted a cross-sectional design to examine the moderating role of initiative on the relationship between intrinsic motivation, and self-efficacy on entrepreneurial intention among Nigerian graduates. A total number of 688 graduate students, including 266 (38.6%) males and 422 (61.4%) females with a mean age of 24.30 years (SD = 3.69), participated in the study. Participants responded to a self-report questionnaire containing Initiative, Intrinsic motivation, self-efficacy, and entrepreneurial intention scales. Results showed that all the variables correlated positively with entrepreneurial intention. Furthermore, initiative moderated the relationship between self-efficacy and entrepreneurial intention [value and start-ups/operations (OPS)], such that high self-efficacy with high initiative showed higher entrepreneurial intention (value). While to those with low self-efficacy and low initiative, high self-efficacy with high initiative showed higher entrepreneurial intention (OPS) compared to low self-efficacy and low initiative. The study highlighted the role of initiative in transforming young graduates’ entrepreneurial intention into full-fledged entrepreneurs.
The P300 event related potential predicts phonological working memory skills in school-aged children
Authors: Harwood Vanessa , Kleinman Daniel , Puggioni Gavino , Baron Alisa
Published: October 2022

The P300 event related potential (ERP) has been cited as a marker of phonological working memory (PWM); however, little is known regarding its relationship to behavioral PWM skills in early school-aged children. The current study investigates the P300 ERP recorded in response to native and non-native (English and Spanish) phoneme contrasts as a predictor of PWM skills in monolingual English-speaking first and second grade children. Thirty-three typically developing children, ages 6–9, completed a battery of phonological processing, language, and cognitive assessments. ERPs were recorded within an auditory oddball paradigm in response to both English phoneme contrasts (/ta/, /pa/) and Spanish contrasts (/t̪a/, /d̪a/). The P300 ERP recorded in response to English phoneme contrasts significantly predicted standard scores on the Nonword Repetition subtest of the Comprehensive Test of Phonological Processing, Second Edition. Spanish contrasts did not elicit a P300 response, nor were amplitude or latency values within the P300 timeframe (250–500 ms) recorded in response to Spanish contrasts related to English nonword repetition performance. This study provides further evidence that the P300 ERP in response to native phonemic contrasts indexes PWM skills, specifically nonword repetition performance, in monolingual children. Further work is necessary to determine the extent to which the P300 response to changing phonological stimuli reflects PWM skills in other populations.
Joint developmental trajectories and temporal precedence of physical function decline and cognitive deterioration: A longitudinal population-based study
Authors: Wei Xiao , Liu Heng , Yang Li , Gao Zihan , Kuang Jinke , Zhou Kexin , Xu Mengfan
Published: October 2022

Objectives

Previous studies primarily explored the unidirectional impact of cognition on physical function. However, the interplay between physical function and cognition and the temporal precedence in their predictive relationships have not been elucidated. We explored the bidirectional mechanism between physical function and cognition in a longitudinal dataset.

Materials and methods

A total of 1,365 participants in the Chinese Longitudinal Healthy Longevity Survey assessed physical function and cognition in 2011 (T1), 2014 (T2), and 2018 (T3) by the Katz scale and the Chinese version of the Mini-Mental State Examination scale, respectively. Changes in the trajectories of physical function and cognition were examined using the latent growth model. The correlational and reciprocal relationships between physical function and cognition were examined using the parallel process latent growth model and autoregressive cross-lagged (ARCL) models.

Results

Cognition and physical function decreased by an average of 0.096 and 0.017 points per year, respectively. Higher physical function was associated with better cognition at baseline (r = 0.237, p < 0.05), and longitudinal changes in physical function and cognition were positively correlated (r = 0.756, p < 0.05). ARCL analysis indicated that physical function at T1 positively predicted T2 cognitive function. However, this predictive relationship reversed between T2 and T3, whereby cognitive function at T2 predicted physical function at T3.

Conclusion

Both physical function and cognition declined over time. Early identification and intervention in physical dysfunction among older adults could be critical to prevent further cognitive impairment and maintain functional independence. Hence, regular functional assessment and individualized care plans are required to achieve healthy aging.
Behavioral intention of mental health practitioners toward the adoption of virtual humans in affect recognition training
Authors: García Arturo S. , Fernández-Sotos Patricia , González Pascual , Navarro Elena , Rodriguez-Jimenez Roberto , Fernández-Caballero Antonio
Published: October 2022

This paper explores the key factors influencing mental health professionals' behavioral intention to adopt virtual humans as a means of affect recognition training. Therapies targeting social cognition deficits are in high demand given that these deficits are related to a loss of functioning and quality of life in several neuropsychiatric conditions such as schizophrenia, autism spectrum disorders, affective disorders, and acquired brain injury. Therefore, developing new therapies would greatly improve the quality of life of this large cohort of patients. A questionnaire based on the second revision of the Unified Theory of Acceptance and Use of Technology (UTAUT2) questionnaire was used for this study. One hundred and twenty-four mental health professionals responded to the questionnaire after viewing a video presentation of the system. The results confirmed that mental health professionals showed a positive intention to use virtual reality tools to train affect recognition, as they allow manipulation of social interaction with patients. Further studies should be conducted with therapists from other countries to reach more conclusions.
Effects of markedness in gender processing in Italian as a heritage language: A speed accuracy tradeoff
Authors: Di Pisa Grazia , Kubota Maki , Rothman Jason , Marinis Theodoros
Published: October 2022

This study examined potential sources of grammatical gender variability in heritage speakers (HSs) of Italian with a focus on morphological markedness. Fifty-four adult Italian HSs living in Germany and 40 homeland Italian speakers completed an online Self-Paced Reading Task and an offline Grammaticality Judgment Task. Both tasks involved sentences with grammatical and ungrammatical noun-adjective agreement, manipulating markedness. In grammatical sentences, both groups showed a markedness effect: shorter reading times (RTs) and higher accuracy for sentences containing masculine nouns as compared to sentences with feminine nouns. In ungrammatical sentences, although both groups were sensitive to ungrammaticality, only HSs showed a markedness effect, that is, they had significantly longer RTs and higher accuracy when violations were realized on feminine adjectives. Proficiency in the HL was a significant predictor of accuracy and RTs at the individual level. Taken together, results indicate that HSs acquire and process gender in a qualitatively similar way to homeland native speakers. However, RT evidence seems to suggest that at least under particular experimental methods, markedness considerations are more prevalent for HSs resulting in a speed-accuracy tradeoff.
Episodic future thinking predicts differences in delay discounting: The mediating role of hippocampal structure
Authors: Guo Yiqun , Wu Huimin , Li Zhangyong , Zhao Le , Feng Tingyong
Published: October 2022

A growing body of evidence suggests that engagement in episodic future thinking (EFT) could reduce delay discounting rates. However, little is known about whether individual differences in the ability of EFT are associated with differences in delay discounting in young adults. In the present study, this association was tested in healthy college students (n = 106, 19.98 ± 1.56 years), and the neural basis underlying this association was also examined using voxel-based morphometry (VBM) method. Behavioral analysis indicated that individual differences in EFT ability can significantly negatively predict discounting rates. VBM analysis first revealed that the EFT score positively correlated with gray matter volume (GMV) of a cluster in hippocampus, while negatively correlated with GMV of a cluster in rostral anterior cingulate cortex. We also found the GMV of a cluster in the mPFC was positively correlated with delay discounting. ROI analysis further revealed that individual differences in delay discounting could be reliably predicted by the GMV in the hippocampus and mPFC. The final mediation analysis showed that the GMV of the hippocampus plays a significant mediating role in the association between EFT and delay discounting, and the indirect effect of the hippocampal GMV accounts for 33.2% of the total effect. Our results suggest that individuals’ EFT ability may be an important determinant of differences in delay discounting, and highlight the hippocampal structure as a neural biomarker for explaining the association between EFT ability and delay discounting.
Adolescent cocaine differentially impacts psychomotor sensitization and epigenetic profiles in adult male rats with divergent affective phenotypes
Authors: Parsegian Aram , García-Fuster M. Julia , Hebda-Bauer Elaine , Watson Stanley J. , Flagel Shelly B. , Akil Huda
Published: October 2022

Adolescent drug use reliably predicts increased addiction liability in adulthood, but not all individuals are equally impacted. To explore the biological bases of this differential reactivity to early life drug experience, we used a genetic rat model of temperament and evaluated the impact of adolescent cocaine exposure on adult psychomotor sensitization. Relative to adult bred low-responder (bLR) rats, bred high-responders (bHR) are more sensitive to the psychomotor-activating effects of cocaine and reinstate drug-seeking behavior more readily following prolonged cocaine exposure and/or abstinence. We found that a 7-day sensitizing cocaine regimen (15 mg/kg/day) during either adolescence or adulthood produced psychomotor sensitization in bHRs only, while a dual cocaine exposure prevented further sensitization, suggesting limits on neuroplasticity. By contrast, adolescent cocaine in bLRs shifted their resilient phenotype, rendering them more responsive to cocaine in adulthood following adolescent cocaine. To begin to explore the neural correlates of these behavioral phenotypes, we assessed two functionally opposite epigenetic chromatin modifications implicated in addiction liability, permissive acetylation (ac) and repressive tri-methylation (me3) on Histone 3 Lysine 9 (H3K9), in four striatal sub-regions. In bHRs, decreased H3K9me3 and increased acH3K9 in the nucleus accumbens (NAc) core associated with cocaine sensitization. In bLRs, the combination of cocaine exposure in adolescence and adulthood, which lead to an increased response to a cocaine challenge, also increased acH3K9 in the core. Thus, adolescent cocaine experience interacts with genetic background to elicit different behavioral profiles relevant to addiction in adulthood, with concurrent modifications in the epigenetic histone profiles in the NAc that associate with cocaine sensitization and with metaplasticity.
The concise measurement of clinical communication skills: Validation of a short scale
Authors: Maaß Ulrike , Kühne Franziska , Heinze Peter Eric , Ay-Bryson Destina Sevde , Weck Florian
Published: October 2022

Objective

There is a lack of brief rating scales for the reliable assessment of psychotherapeutic skills, which do not require intensive rater training and/or a high level of expertise. Thus, the objective is to validate a 14-item version of the Clinical Communication Skills Scale (CCSS-S).

Methods

Using a sample ofN= 690 video-based ratings of role-plays with simulated patients, we calculated a confirmatory factor analysis and an exploratory structural equation modeling (ESEM), assessed convergent validities, determined inter-rater reliabilities and compared these with those who were either psychology students, advanced psychotherapy trainees, or experts.

Results

Correlations with other competence rating scales were high (rs > 0.86–0.89). The intraclass correlations ranged between moderate and good [ICC(2,2)= 0.65–0.80], with student raters yielding the lowest scores. The one-factor model only marginally replicated the data, but the internal consistencies were excellent (α = 0.91–95). The ESEM yielded a two-factor solution (CollaborationandStructuring and Exploration Skills).

Conclusion

The CCSS-S is a brief and valid rating scale that reliably assesses basic communication skills, which is particularly useful for psychotherapy training using standardized role-plays. To ensure good inter-rater reliabilities, it is still advisable to employ raters with at least some clinical experience. Future studies should further investigate the one- or two-factor structure of the instrument.
Lead exposure as a causative factor for metabolic associated fatty liver disease (MAFLD) and a lead exposure related nomogram for MAFLD prevalence
Authors: Yang Chenyu , Li Yuanyuan , Ding Ran , Xing Huiwu , Wang Ruijue , Zhang Mingman
Published: October 2022

The relationship between lead exposure and neurological disorders has been extensively studied, but the effects of lead exposure on hepatotoxicity are unknown. Metabolically related fatty liver disease (MAFLD) is an update of previous non-alcoholic fatty liver disease (NAFLD). It redefines the diagnostic conditions and emphasizes metabolic factors while considering non-alcoholic factors. Lead can affect the endocrine system and metabolism, so we believe that lead exposure may contribute to MAFLD. 41,723 individuals who had undergone blood lead testing from 2005 to 2018 in the National Health and Nutrition Examination Survey (NHANES) database were selected for this study. The characteristics of population lead exposure in the last decade or so, the effect of lead exposure on liver function and whether lead exposure can cause MAFLD were analyzed. Co-variates were adjusted according to age, ethnicity, body mass index (BMI), waist circumference, visceral adiposity index (VAI), poverty indices (PIR), diabetes, hypertension, and hyperlipidemia. The results showed that blood lead concentrations stabilized at a low level after a decreasing trend from year to year. The differences in blood lead concentrations were associated with differences in age, sex, race, education level, and PIR. Lead exposure was an independent risk factor for MAFLD, and lead and nine other factors were used as independent risk factors for MAFLD, so a nomogram was established to predict the prevalence probability of MAFLD.
Situation awareness in intensive care unit nurses: A qualitative directed content analysis
Authors: Ghaderi Chiman , Esmaeili Roghayeh , Ebadi Abbas
Published: October 2022

Background

Situation awareness (SA) is an essential cognitive construct to create positive patient safety outcomes. SA of the nurses in the intensive care unit (ICU), where conditions may change rapidly, is particularly important. The present study aimed to explain the perception and experience of SA in ICU nurses based on Endsley's SA model.

Materials and methods

This qualitative directed content analysis was conducted on nurses in six hospitals in Tehran, Iran, from December 2020 to July 2021. Twenty-seven ICU nurses were selected using purposive sampling. Data were collected by semi-structured interviews and field observations. The data were analyzed based on the Elo and Kyngas method modified by Assarroudi et al. COREQ checklist was used to report the research.

Results

The concept of SA in ICU nurses, based on Endsley's model, includes perception of patients' clinical cues, perception of the human environment, perception of the physical environment, and perception of the organizational environment as generic categories of the perception of the elements in the environment. SA in ICU nurses also includes the main categories of comprehension the current situation through a sense of salience and interpretation of cues and projection the future situation through the prediction of patient status into the near future and environmental foresight.

Conclusion

Findings have further developed the concept of SA in ICU nurses based on Endsley's SA model. The insights and knowledge gained from this study can be useful for future practice, education, and research on SA among ICU nurses.
Sensor-based outcomes to monitor everyday life motor activities of children and adolescents with neuromotor impairments: A survey with health professionals
Authors: Rast Fabian Marcel , Labruyère Rob
Published: October 2022

In combination with appropriate data processing algorithms, wearable inertial sensors enable the measurement of motor activities in children's and adolescents' habitual environments after rehabilitation. However, existing algorithms were predominantly designed for adult patients, and their outcomes might not be relevant for a pediatric population. In this study, we identified the needs of pediatric rehabilitation to create the basis for developing new algorithms that derive clinically relevant outcomes for children and adolescents with neuromotor impairments. We conducted an international survey with health professionals of pediatric neurorehabilitation centers, provided them a list of 34 outcome measures currently used in the literature, and asked them to rate the clinical relevance of these measures for a pediatric population. The survey was completed by 62 therapists, 16 doctors, and 9 nurses of 16 different pediatric neurorehabilitation centers from Switzerland, Germany, and Austria. They had an average work experience of 13 ± 10 years. The most relevant outcome measures were the duration of lying, sitting, and standing positions; the amount of active self-propulsion during wheeling periods; the hand use laterality; and the duration, distance, and speed of walking periods. The health profession, work experience, and workplace had a minimal impact on the priorities of health professionals. Eventually, we complemented the survey findings with the family priorities of a previous study to provide developers with the clinically most relevant outcomes to monitor everyday life motor activities of children and adolescents with neuromotor impairments.