bluuebunny/crossref_metadata_2025 huggingface dataset:
Transcription Elongation Factor A-like 7 is a Stemness-Related Prognostic Factor in Gastric Cancer
Authors: Qian Haisheng , Gao Xin , Ma Rui , Li Wenjie , Yang Zhen , Zhang Guoxin , Li Xuan
Published: April 2021

Background: Stemness is described as the potential for self-renewal and differentiation from the cell-of-origin. A previous study calculated the mRNA expression-based stemness index (mRNAsi) based on a one-class logistic regression machine learning algorithm for describing stemness features of cancer. We aim to identify stemness-related prognostic genes in gastric cancer (GC) based on mRNAsi using bioinformatics analysis.Methods: The WGCNA analysis was performed to find the relevant gene modules to mRNAsi. Gene ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) signaling pathways annotation analysis were performed on genes in blue module. The overall survival analysis, univariate Cox regression analysis and the least absolute shrinkage and selection operator (LASSO) regression model were used to identify prognostic genes highly associated with survival. The multivariate Cox regression analysis was performed to analysis prognostic factors. The nomogram was constructed according to the result of multivariate analysis. qPCR, Western Blot and IHC staining were appliedResults: The mRNAsi of tumors is higher than normal tissues, and there was a significant difference in overall survival (OS) between the high and low mRNAsi GC groups. TCEAL7 was selected to be the key gene associated with mRNAsi and prognosis according to the result of least absolute shrinkage and selection operator (LASSO) Cox regression. The expression level of TCEAL7 was lower in tumors than in normal tissues, but high TCEAL7 level group showed a worse OS than low TCEAL7 level group in GC. Based on the result of multivariable Cox regression analysis which including TCEAL7 and clinical characteristics, a nomogram for predicting GC 1-, 3-, and 5-year survival was established. The C-index and the AUC (Area Under Curve) of the model indicated that the model has a good discrimination ability. Additionally, the calibration curves of 3- and 5-year OS rates showed the model fits well. The experimental validation of the expression of TCEAL7 in GC and normal tissues were consistent with the above.Conclusions: In summary, we verified mRNAsi was associated with the prognosis of GC patients. And TCEAL7 was finally identified as the key gene correlated with stemness features and prognosis in GC.
Pressure Infiltration of Molten Aluminum for Densification of Environmental Barrier Coatings
Authors: Dong Lin , Liu Mei-Jun , Zhang Xiaofeng , Zhuo Xueshi , Fan Jiafeng , Yang Guan-Jun , Zhou Kesong
Published: April 2021

Environmental barrier coatings (EBCs) effectively protect ceramic matrix composites (CMCs) from harsh engine environment, especially steam and molten salts. However, open pores inevitably formed during deposition process provide transport channels for oxidants and corrosives and lead to premature failure of EBCs. This work proposed a pressure infiltration densification method that blocked these open pores in the coatings. Results showed that it was difficult for aluminum to infiltrate spontaneously, but with the increase of external gas pressure and internal vacuum simultaneously, the molten aluminum obviously moved forward, but finally stopped infiltrating at a depth of a specific geometry. Based on the wrinkled zigzag pore model, a mathematical relationship between the critical pressure and the infiltration depth and the pore intrinsic geometry was established. Infiltration results confirmed this relationship, indicating that for a given coating, a dense thick film can be obtained by adjusting the internal and external gas pressures to drive the melt infiltration.
Studies of the Seasonal Prediction of Heavy Late Spring Rainfall Over Southeastern China
Authors: Zhang Shouwen , Wang Hui , Jiang Hua , Ma Wentao
Published: April 2021

The late spring rainfall may account for 15% of the annual total rainfall, which is crucial to early planting in southeastern China. A better understanding of the precipitation variations in the late spring and its predictability not only greatly increase our knowledge of related mechanisms, but it also benefits society and the economy. Four of the current models participating in the North American Multi-Model Ensemble (NMME) were selected to study their abilities to forecast the late spring rainfall over China and the major sources of this heavy rainfall from the perspective of the sea surface temperature (SST) field. We found that the models have better abilities to forecast the heavy rainfall over the middle and lower reaches of the Yangtze River region (MLYZR) with only a 1-month lead time, but they failed for a 3-month lead time since the occurrence of the heavy rainfall was inconsistent with the observations. The observations indicate that the warm SST anomalies in the tropical eastern Indian Ocean are vital to the simultaneously heavy rainfall in the MLYZR in May, but an El Niño event is not a necessary condition for determining the heavy rainfall over the MLYZR. The heavy rainfall over the MLYZR in May is always accompanied by warming of the northeastern Indian Ocean and of the northeastern South China Sea (NSCS) from April to May in the models and observations, respectively. In the models, El Niño events may promote the warming processes over the northeastern Indian Ocean, which leads to heavy rainfall in the MLYZR. However, in the real world, El Niño events are not the main reason for the warming of the NSCS, and further research on the causes of this warming is still needed.
Stress Hormone Response to Instrumented Lumbar Spine Fusion Surgery
Authors: Repo Jussi P. , Neva Marko , Häkkinen Keijo , Pekkanen Liisa , Metso Saara , Häkkinen Arja
Published: April 2021

The purpose of the present study was to investigate stress, anabolic and catabolic hormonal levels and their association with interleukin 6 (IL-6) cytokine in patients undergoing lumbar spine fusion (LSF) surgery. Blood samples were collected preoperatively, and at 1, 3, 42 and 90 days postoperatively (POD) from 49 LSF patients with a mean (SD) age of 62 (11) years. Serum concentrations of adrenocorticotropic hormone (ACTH), cortisol, growth hormone (GH), insulin-like growth factor 1 (IGF-1), testosterone, sex hormone-binding globulin (SHBG) and IL-6 were analyzed. In women, cortisol concentration rose above baseline values despite a fall in ACTH levels. GH showed a decrease on PODs 1 and 3 whereas IGF-1 levels remained stable. In males, SHBG increased, and both testosterone and free testosterone showed a decrease during PODs 1-3. The other hormone concentrations had returned to normal by PODs 42 or 90, except for IGF-1, which remained above the baseline value on PODs 42 and 90. IL-6 correlated significantly with cortisol (p <0.001) level on POD 1. The results suggest that hypercortisolism after operative stress is caused by cytokine-induced non-ACTH-driven cortisol production or reduced cortisol breakdown suppressing the production of ACTH via feedback inhibition. Furthermore, GH levels decrease rapidly.
Plasma Membrane Effects of Sphingolipid-Synthesis Inhibition by Myriocin in CHO Cells: A Biophysical and Lipidomic Study
Authors: Monasterio Bingen G. , Jiménez-Rojo Noemi , García-Arribas Aritz B. , Riezman Howard , Goñi Félix M. , Alonso Alicia
Published: April 2021

Two main strategies for establishing the cellular effects of a given enzyme activity suppression are (a) the use of a stably mutated cell line that lacks a functional gene, or (b) treating the wild type with an inhibitory compound that affects the same gene-product protein. In this work, myriocin was used to block the serine palmitoyltransferase (SPT) enzyme of CHO cells and the subsequent biophysical changes in membranes were measured and compared with results obtained with a genetically modified CHO cell line containing a defective SPT (the LY-B cell line). Similar effects were observed with both approaches: sphingomyelin values were markedly decreased in myriocin-treated CHO cells and, in consequence, their membrane molecular order (measured as laurdan general polarization) and mechanical resistance (AFM-measured breakthrough force values) happened to be lower than in the native, non-treated cells. Cells treated with myriocin reacted homeostatically to maintain membrane order, synthesizing more fully saturated and less polyunsaturated glycerophospholipids than the non-treated ones, although they achieved it only partially, their plasma membranes remaining more fluid and less penetrable than those from the control cells.
Neuro-Oncology Clinicians’ Attitudes and Perspectives on Medical Assistance in Dying
Authors: Climans Seth Andrew , Mason Warren P , Edelstein Kim , Variath Caroline , Bell Jennifer A H
Published: April 2021

IntroductionMedical assistance in dying (MAiD), also known as physician-assisted death, is currently legal in several locations across the globe. Even more jurisdictions are considering legalizing MAiD. Brain cancer or its treatments can lead to cognitive impairment, which can impact decision-making capacity. In most jurisdictions patients are assessed for mental capacity to inform MAiD eligibility. We sought to explore worldwide neuro-oncology clinicians’ attitudes and perspectives on MAiD, including interpretation of decision-making capacity for patient MAiD eligibility.MethodsAn online survey was distributed to members of national and international neuro-oncology societies. We asked questions about decision-making capacity and MAiD, in part using hypothetical patient scenarios. Multiple choice and free-text responses were captured.ResultsThere were 125 survey respondents. Impaired cognition was identified as the most important factor that would signal a decline in patient capacity. At least 26% of survey respondents had moral objections to MAiD. Fewer clinicians were willing to support a MAiD decision for patients with lower-grade tumors and better performance status.Conclusionshile there are differing opinions on the moral permissibility of MAiD in general and for neuro-oncology patients, most clinicians agree that capacity must be assessed carefully before a decision is made. Further, some patients with brain tumors are not generally thought to be MAiD-eligible due to the nature of their specific diseases. These results can inform assessments of patient capacity in neuro-oncological practice in jurisdictions where MAiD is legal.
Effect and optimization of NaOH combined with Fenton pretreatment conditions on enzymatic hydrolysis of poplar sawdust
Authors: Li Fei , Lu Xiaohong , Li Yiming , Zhou Xia , Zhao Zhezhen , Liu Ping
Published: April 2021

In this study, the effects and mechanism of pretreatments of three types of chemical reagents combined with Fenton on poplar sawdust were studied and the optimization of enzymatic hydrolysis conditions was conducted using response surface methodology. The results showed that cellulase and hemicellulase had the best hydrolysis effect after NaOH-Fenton pretreatment, which were 63.73% and 29.29%, respectively. The optimal process of poplar substrate was to react in 1% NaOH at 100 ℃ for 1 h, then placed in the Fenton reaction system of 0.2 mmol Fe2+ and 25 mmol H2O2 for 7 h, and finally subjected to enzymatic hydrolysis for 72 h at 52 ℃, with a liquid-solid ratio of 33 and 15 μL/g of β-glycosidase. Under this condition, the enzymatic hydrolysis rates of cellulase and hemicellulase reached 86.65% and 43.9%, respectively. In conclusion, the combination of NaOH and Fenton pretreatment can effectively promote the enzymatic hydrolysis of poplar sawdust, which has great potential in the production of cellulosic ethanol.
Effect of a Structured Checklist on Quality of Intraoperative Change-of-shift Handoffs: A pre-post control study
Authors: Nasiri Ebrahim , Lotfi Mojgan , Mahdavinoor Seyyed Muhammad Mahdi , Rafiee Mohammad hossein
Published: April 2021

Objective: Handoff without a structured format is prone to the omission of information and could be a potential risk to patient safety. We sought to determine the effect of a structured checklist on the quality of intraoperative change of shift handoff between scrubs and circulating.Methods: We conducted a pre-post control intervention study on operating room wards of two teaching hospitals from February to November 2020. This research was conducted in three stages as follows: assessing the current situation (as a group before the intervention), performing the intervention and evaluating the effect of using a checklist on handoff quality after the intervention in two groups: with and without checklist. We examined the quality of handoff between scrub and circular personnel in terms of handoff duration and quality, omission of information as well as changes in personnel satisfaction. Data were analyzed using SPSS software version 26.Results: A total of 120 handoffs were observed and evaluated. After intervention in the group using the checklist, the percentage of information omission in surgical report was decreased from 19.5 to 12.1% between scrubs (P<0.00) and from 16.8 to 14.1% between circulating (P <0.03). Also, in the role of scrub, the mean overall score of handoff process quality was significantly higher after the intervention (x̄=7±1.5) than before it (x̄=6.5±0.9) (p<0.02). In the role of circulating, despite the positive effect of overall score checklist, no significant difference was observed (p<0.08). The use of checklist significantly increased the handoff duration between scrubs (p<0.03) and circulating (p<0.00).Conclusion: Structured checklist has a positive impact on improving the quality of communication between the surgical team, reducing the information omission rate and increasing the satisfaction.
Climate Smart Agriculture from the intensive vegetable farmers perspectival
Authors: Adusei Frank Yeboah
Published: April 2021

Climate Smart Agriculture (CSA) has been identified as the best way forward to contribute to mitigating climate change for enhanced agriculture. The study was conducted in Asokwa Municipal in the Ashanti region of Ghana as a case study with the following objectives; to identify existing CSA practices adopted by vegetable farmers; to evaluate existing institutions and their role in facilitating the adoption of CSA practices and to establish the likely factors that may promote or inhibit adoption of CSA practices. Purposive sampling was used to select twenty-seven participants due to restrictions on COVID-19 and limited resources. The significance of this method is that participants are selected by virtue of their capacity to provide rich-textured information relevant to the phenomenon under study. Results from the field showed that the commonly adopted CSA practices were improved crop varieties, irrigation and manure management scoring 100% each followed by crop rotation (66.7%). The least adopted practices, from the highest to the lowest were agroforestry (12.5%), mulching and rain harvesting (8.3%) each and compost application with 4.2%. The key factors inhibiting the adoption of CSA consist of insufficient information, water scarcities and financial constraints. The conclusion drawn was that the Agricultural sector must become climate-smart to successfully tackle current food security and climate change challenges. Beyond doubt, it will require management and governance practices based on ecosystem approaches that involve multi-stakeholder and multi-sectoral coordination and cooperation.
Recovery of autologous sickle cells by hypotonic wash
Authors: Wilson E. , Kezeor K. , Crosby M.
Published: April 2021

It is important to isolate autologous red blood cells (RBCs) from transfused RBCs in samples from recently transfused patients to ensure that accurate serologic results are obtained. Typically, this isolation can be performed using methods that separate patient reticulocytes from transfused, older donor RBCs. Patients with sickle cell disease (SCD), however, characteristically have RBCs with altered membrane and morphological features, causing their RBCs to take on a sickle-shape appearance different from the biconcave disc-shape appearance of “normal” RBCs. These characteristics enable the use of hypotonic saline solution to lyse normal RBCs while allowing “sickle cells” to remain intact. Because many patients with SCD undergo frequent transfusions to treat their condition, the use of hypotonic saline solution provides a rapid method to obtain autologous RBCs for serologic testing from this patient population using standard laboratory equipment and supplies.
Immunohematology

2018;34: 16–18.
Mixed-field agglutination observed in column agglutination testing is not always associated with the A3 subgroup
Authors: Anukul N. , Leetrakool N. , Tanan P. , Palacajornsuk P. , Klangsinsirikul P.
Published: April 2021

Mixed-field agglutination (MFA) can be observed in forward typing of samples from A3 individuals with serologic ABO typing methods. The results of column agglutination testing (CAT) and tube agglutination testing using different antibody clones can be discordant. In this report, we reveal our experience using polymerase chain reaction–sequence-based typing (PCR-SBT) of ABO exon 7 to clarify serologic method discordance of A subgroup blood typing in Northern Thai donors. A total of 21 group A blood donors with either MFA or weak agglutination on routine ABO CAT were recalled. CAT was repeated with human and monoclonal anti-A, and tube agglutination testing with monoclonal anti-A and PCR-SBT of ABO exon 7 was performed. A total of 13 of the 21 donors returned, and ABO CAT with human anti-A was repeated. Eleven samples showed MFA suspected to be the A3 subgroup, and two samples showed 2+ strength suspected to be the Aweak subgroup. When tube agglutination testing using monoclonal antibody was performed, MFA was not observed in 9 of 11 samples with previously observed MFA from routine CAT, which were then interpreted as A2. From PCR-SBT performed in only exon 7 of the ABO gene, 7 of 13 sample results were consistent with ABO*A2 or ABO*AW alleles. Two samples suspected to be A2 or A3 had an ABO*AW allele. In two samples suspected to be Aweak, no mutation was detected in ABO exon 7, suggesting genetic variation elsewhere in the gene. Although other coding exons were not examined, in the alleles that could be assigned, ABO*A3 alleles were found less frequently than would be predicted from the serologic findings. These findings suggest that when MFA in routine CAT is observed, an A3 subgroup cannot be presumed. Caution should be exercised when MFA is noted in routine CAT.
Immunohematology

2018;34:49–56.
Clinical significance of antibodies to antigens in the Scianna, Dombrock, Colton, Landsteiner-Weiner, Chido/Rodgers, H, Kx, Cromer, Gerbich, Knops, Indian, and Ok blood group systems
Authors: Lejon Crottet S.
Published: April 2021

This article reviews information regarding the clinical significance of antibodies to antigens in the Scianna, Dombrock, Colton, Landsteiner-Wiener, Chido/Rodgers, H, Kx, Cromer, Gerbich, Knops, Indian, and Ok blood group systems. Like most blood group systems, antibodies to many of the antigens in these groups are rarely encountered because of the high prevalence of the associated antigens in most populations. For many, the clinical significance—that is, the potential to cause reduced survival of transfused antigen-positive red blood cells or a transfusion reaction (e.g., anti-Ge2, anti-H) and/or hemolytic disease of the fetus and newborn (e.g., anti-Coa, anti-Ge3)— has been documented. Some of these antibodies are not always clinically significant, and because of the high prevalence of the antigen, antigen-negative blood may be extremely difficult to find (e.g., anti-LW, anti-Inb). The use of a monocyte monolayer assay may be helpful when making transfusion decisions for patients with these antibodies. For others, their prevalence is so rare that information on the clinical significance of their antibodies is not available (e.g., anti-Co4, anti-Ok).
Immunohematology

2018;34:103–108.
A delayed and acute hemolytic transfusion reaction mediated by anti-c in a patient with variant RH alleles
Authors: Walters T.K. , Lightfoot T.
Published: April 2021

The Rh system is the most complex of the human blood groups. Of the 55 antigens that have been characterized, the system’s principal antigens D, C, E, c, and e are responsible for the majority of clinically significant Rh antibodies. In the last few years, advancements in molecular testing have provided a wealth of information on the genetic diversity of the Rh locus. This case report describes a patient with variant RHD*DAR alleles inherited in conjunction with two compound heterozygote RHCE*ceEK/RHCE*ceAR alleles. The patient was previously alloimmunized to D, C, and E and possibly hrS. Further transfusion of D–C–E–K– RBCs resulted in a suspected acute hemolytic transfusion reaction and the subsequent identification of anti-c. Monocyte monolayer assay testing suggests clinical significance with a range of 29.5–38.5 percent reactive monocytes.
Immunohematology

2018;34:109-112.
An update on the GLOB blood group system (and former GLOB collection)
Authors: Ricci Hagman J. , Westman J.S. , Hellberg Å. , Olsson M.L.
Published: April 2021

The main change that has occurred in the GLOB blood group system since the GLOB review published in this journal in 2013 is the addition of an antigen. The high-prevalence PX2 antigen, originally recognized as the x2 glycosphingolipid, is expressed on red blood cells of most individuals and is elevated in the rare PP1Pk-negative p blood group phenotype. P synthase, encoded by B3GALNT1, was found to elongate paragloboside to PX2 by adding the terminal β3GalNAc moiety. Hence, PX2 was moved from the GLOB collection to the GLOB system. The presence of naturally-occurring anti-PX2 was noted in P1
k and P2
k individuals exhibiting nonfunctional P synthase. Although the clinical significance of this specificity remains unclear, a recommendation to avoid transfusing Pk patients with p phenotype blood has been made. Currently, 13 mutations at the highly conserved B3GALNT1 locus have been found to abolish P synthase function and are recognized as null alleles by the International Society of Blood Transfusion. A new allele with a missense mutation but resulting in normal expression of P has been assigned GLOB*02. Finally, the GLOB collection was made obsolete after the move of LKE antigen to the 901 series.
Immunohematology

2018;34:161–163.
ANALISIS KOEFISIEN ABSORPSI DARI MATERIAL BERBAHAN DASAR LIMBAH KAIN PERCA DAN PELEPAH PISANG
Authors: Noviandri Patricia P. , Harjani Centaury , Astuti Adindra M. K.
Published: April 2021

Title: Coefficient Absorpsi Analysis from Patchwork Material and Banana Midrib
Preliminary research related composite waste material and nature has done. This research continues the previous research with a more accurate testing method using impedance tubes. Material samples made in this study are samples that have a greater NR (Noise Reduction) value in previous studies. Material samples were made as many as 4 samples with different substance variables, namely fine composite samples, rough composites, banana midrib, and combinations (fine composites and banana midrib). Three samples were mold using a tube with a diameter of 30 mm and a height of 70 mm while one sample combination was a material that was combined to reach a height of 50 mm. From the test results, the addition of cavity affects the absorption coefficient value, therefore the use of this material in buildings must use an air cavity so that the absorption coefficient value increases. Samples that have character as sound absorbing material are fine composite materials, banana fronds, and combination materials because they have an absorption coefficient value (?) of more than 0.30. The combination material has the highest NRC value among the other samples that is equal to 0.57.
DAYA DUKUNG LINGKUNGAN TERKAIT PENGOLAHAN LIMBAH BATIK DI KAMPUNG BATIK GIRILOYO, KABUPATEN BANTUL, YOGYAKARTA
Authors: Muliasari I Gusti Ayu Dwi , Widiastuti
Published: April 2021

Title: Carrying Capacity Related to Batik Waste Processing in Giriloyo Batik Village, Bantul District, Yogyakarta
Yogyakarta is one of the areas in Indonesia that still maintains its cultural heritage, one of the cultural heritages that is still a source of pride is Batik. This cultural heritage is increasingly in demand by many people because of its very diverse motives and usually these batik motifs describe the characteristics of an area so that they can be used as souvenirs. This higher market demand has made batik production increase, causing new problems, namely environmental pollution as a result of the waste of batik making itself. The purpose of this study was to determine how to dispose of batik waste in Kampung Batik Giriloyo, Yogyakarta, and to analyze its impact on environmental quality. This research uses qualitative research methods with a case study approach. The results showed that some of the batik home producers still dispose of their waste directly into the river, some of them dispose of it in wells without filtration, and some have disposal sites that meet standards. Discharge in the river results in dirty river water and causes the fish in it to die. Direct dumping into the soil causes the surrounding plants to die. Meanwhile, disposal in soil wells without filtration causes soil contamination and there is a concern that it will contaminate nearby well water.
Continuous combined low-dose hormone replacement therapy in perimenopause: an algorithm of choice
Authors: Yakushevskaya O. V.
Published: April 2021

In the age of broad medical options, women’s health has received sufficient attention. The different periods of a woman’s life are characterised by specific physiological changes, based on the age-related characteristics of the reproductive system. The onset of menopause can have a negative impact on health in varying degrees. Clinicians have a clear understanding of the effects of estrogen deficiency and the therapeutic options for managing it with menopausal hormone therapy (MHT) and alternative methods of treatment. However, to date, methods for optimising and individualising the correction of menopausal disorders continue to improve. The individualization of MHT is aimed at increasing the efficacy of menopausal management and minimizing possible adverse events. Individualization is based on the selection of a hormone drug taking into account age, menopausal status, somatic health of the woman and her main complaints against the background of estrogen deficiency. The next stage of transformation of MHT concerned the composition of the drugs and the doses of their components. The evolution of the estrogenic component began with the use of conjugated estrogens, whose metabolism is not fully clarified, and stopped at the production of bioidentical estrogens (17p-estradiol and estradiol valerate), which in their structure are as close as possible to ovarian estradiol. The type, dose and combination of estrogens and progestogens determine the severity and specificity of the effect of the hormone. This article will present a clinical case study of the low- and ultra-low-dose combination of 17p-estradiol and dydrogesterone (E/DG).
Modern aspects of complex therapy for recurrent genital herpes
Authors: Tyutyunnik V. L. , Kan N. E. , Tyutyunnik N. V. , Soldatova E. Е.
Published: April 2021

Incidence of reported cases of genital herpes in different countries has increased dramatically during the last years, which is essentially caused by spread of asymptomatic or atypical types of herpes. Frequent relapses of herpes infection are characterized by changes in the immunological status. The authors of presented article analysed the findings of foreign and domestic studies on the treatment of the disease, systematized information about the features of antiviral chemotherapy drugs used in the treat-

ment of genital herpes, identified criteria for the efficacy and duration of suppressive therapy, and the feasibility of its combination with courses of immunomodulatory drugs. The article summarizes the authors’ own experience in the treatment of genital herpes in 50 patients, who were randomly divided into 2 groups: (active treatment) group 1 consisted of 25 women who received antiviral (acyclovir and its analogues) and immunomodulatory therapy (aminodihydrophthalasindione sodium, suppositories), (comparison) group 2 consisted of 25 patients who received antiviral therapy only. The following were criteria for the inclusion of patients: genital herpes history, clinical presentation of genital herpes during the prodromal period or exacerbation, and no more than 48 hours after the rash occurred.

The results of evaluation of the treatment effectiveness showed that the vesicles disappeared and the crusts formed during aminodihydrophthalasindione sodium therapy in the active treatment group of patients as early as Day 2-5 after starting treatment, while the acute phase of local inflammation in the comparison group was reversed only by Day 4-5. Complete reepitheli-alization occurred in the active treatment and comparison groups by Day 6-7 of the treatment. At the same time, the PCR test did not detect HSV-2 in smears in 24 (96.0%) women in the active treatment group and in 25 (88.0%) women in the comparison group. No one had any fresh rashes in the course of treatment with the drug. In addition, the authors reported prolongation of clinical remission in the active treatment group up to 5 months (up to 55 days on average).

As can be seen from the above, the inclusion of immunomodulatory drugs in the combination specific antiviral therapy leads to faster achievement of the clinical effect, allows to reduce frequency of relapses and improves the patients’ quality of life.
A challenge of fertility preservation in the setting of cervical cancer - a clinical case
Authors: Dmitrieva I. E. , Martirosyan Y. O. , Dzhanashvili L. G. , Dementyeva V. O. , Adamyan L. V. , Nazarenko T. A.
Published: April 2021

The issue of fertility preservation in patients with cervical cancer is getting more and more common considering the improved effectiveness of early diagnosis and treatment of cancer. There is a number of evidence-based tactics available to the patients with diagnosed cervical cancer. These tactics have been proved effective and include methods such as ovarian transposition; oocyte, embryo and ovarian tissue cryopreservation. Nonetheless, there are no existing medical algorithms to define the priority of actions that should be taken in such cases of restricted time. The objective of this clinical case report is to highlight an existing concern towards the decision-making process regarding fertility preservation in patients with cervical cancer.

In this paper we report a clinical case of fertility preservation tactics in a patient undergone ovarian transposition. We pay attention to particular features of the controlled ovarian stimulation (COS) and oocyte retrieval process typical for such patients. The article discusses the subject of COS and oocyte retrieval effectiveness compared to patients who did not undergo ovarian transposition. The 35-year old patient presented 9 month after ovarian transposition to perform oocyte cryopreservation. As a result of COS 3 oocytes were aspirated, compared to 20 oocytes in another patient of comparable age and medical history, but with no ovarian transposition performed.

Regarding particular conditions constraining ovarian stimulation and oocyte retrieval after ovarian transposition, we recommend to consider the possibility of performing oocyte/embryo cryopreservation before ovarian transposition in patients with cervical cancer. Prioritizing oocyte and embryo cryopreservation in case of having sufficient time before treatment could significantly improve possibilities of achieving genetically related offspring in a long-term perspective.
Hormonal profile of patients with deep infiltrative endometriosis
Authors: Safronova A. S. , Buralkina N. A. , Chuprynin V. D. , Ivanets T. Yu.
Published: April 2021

Introduction. About 30 to 50% of women with endometriosis complain of infertility. The incidence of infertility in all localizations of endometriosis is about 3-4 times higher than that in the general population.

Objective of the study: estimate the hormonal profile of patients with deep infiltrative endometriosis (DIE) based on the study of the level of AMH, FSH, LH, progesterone, estradiol, prolactin in the serum of peripheral blood, conduct a comparative analysis of the level of these hormones between groups of patients with DIE and tuboperitoneal infertility.

Materials and methods: the main study group consisted of 99 patients of reproductive age (from 18 to 40 years) with DIE, the comparison group consisted of 18 patients with tuboperitoneal infertility. The patients of the main group were divided into 2 subgroups: subgroup IA - DIE with colorectal endometriosis (n = 63), subgroup IB - DIE without colorectal endometriosis (n = 36). AH patients underwent determination of the level of AMH, LH, FSH, progesterone, estradiol, prolactin in the serum of peripheral blood before surgery.

Results and discussion: a significant decrease in AMH level was revealed in patients with DIE. The level of AMH in the main and comparison groups was 1.80 and 3.28 ng/ml, respectively (p = 0.01). At the same time, subgroup IA has a more decreased AMH level (1.37 ng/ml) than in the comparison group (p = 0.003). In subgroup IB (the median AMH level - 2.50 ng/ml) were no significant differences with the comparison group (p = 0.14). It was also noted that in the group of patients with colorectal endometriosis (subgroup IA) the percentage of patients with reduced ovarian reserve (AMH 0.01 - 1.0 ng / ml) was significantly higher than in the comparison group (30.2% versus 5, 6%, p = 0.03), there were no significant differences between the subgroups. There were no significant differences in the level of LH, FSH, E2, prolactin in serum between the groups (p> 0.05).

Assessment of the level of anti-mullerian hormone, as the most accurate marker of ovarian reserve, revealed a significant decrease in AMH levels in patients with DIE, more decreased in the group of patients with colorectal endometriosis. Every 3rd patient with DIE had an AMH level of less than 1, which is a risk factor for a “poor” response to ovarian stimulation according to the Bologna criteria.

Conclusion.A significant decrease in the level of serum AMH, along with a high percentage of previous surgeries for endometriosis, including ovarian resections, may be one of the factors in a decrease in the reproductive potential of patients with DIE.
Ovarian effects of vitamin D: a systematic review
Authors: Chernukha G. E. , Yakushevskaya O. V.
Published: April 2021

The history of the discovery of vitamin D (VD) has crossed 100 years. Initially, VD deficiency was limitedly associated with the development of rickets in children. Later, on the basis of a number of studies, it was found that VD deficiency is a risk factor for osteoporosis in men and women. However, the traditional concept of VD as the main regulator of calcium-phosphorus metabolism has undergone dramatic changes over the past two decades. A prerequisite for revising the role of VD and the spectrum of its biological properties was the identification of the distribution of its specific receptors in the body. VD receptors are expressed in the intestine, thyroid and parathyroid glands, kidneys, ovaries, uterus, placenta, hypothalamus, pituitary gland and play a vital function not only in calcium homeostasis. Accordingly, the list of target organs for VD action has been modified. VD deficiency, calculated on a pandemic scale, is a factor that increases the risk of developing not only osteoporosis, but also a number of other diseases. Observational and epidemiological studies have shown that reproductive system disorders are very often combined with VD insufficiency / deficiency. Subsequent scientific research aimed at establishing the role of VD in the formation of a particular pathology of the reproductive system has shown very ambiguous results. VD is recognized as an essential component of maintaining a woman’s reproductive health. Of course, the presence of an established association is not evidence of a causal relationship. There is a need for additional testing. This review focuses on the effect of VD on ovarian function and the formation of various dysfunctional conditions.
Clinical and pathogenetic variants of fetal growth restriction with different periods of manifestation
Authors: Lipatov I. S. , Tezikov Yu. V. , Amosov M. S. , Zumorina E. M.
Published: April 2021

The article presents data on the clinical and pathogenetic variants of fetal growth restriction (FGR). FGR is one of the typical clinical manifestations of large obstetric syndromes, is associated with a high perinatal morbidity and has a significant impact on the quality and duration of human life. The emphasis is made on the differences in pathogenesis, the features of prediction, diagnosis, obstetric management and the assessment of perinatal outcomes in the early and late phenotype of the FGR. The review includes materials from domestic and foreign scientific literature that found in eLibrary and PubMed on this topic and published for the last 10 years. This article discusses the role of the formation of the embryo(feto)placental system under the influence of existing periconceptional risk factors in the implementation of various phenotypes of FGR. An analysis of the literature shows that the fetal growth potential, which genetically and epigenetically determined, changes during pregnancy

depending on maternal, placental and fetal factors, which ultimately determine the final weight-growth parameters of the newborn. The issues of informativeness of clinical, laboratory and instrumental predictors, diagnostic criteria, the choice of rational obstetric management in case of FGR of various periods of manifestation are discussed in this article. Convincing data on the perinatal and long-term consequences of intrauterine growth restriction are presented. The pathogenetic variant of FGR determines the features of the functioning of the immune system, has a significant impact on the programming of metabolic and endocrine processes, the formation of fetal brain structures. Identification of pregnant women at risk for the development of FGR of various periods of manifestation, timely diagnosis, selection of the timing and method of delivery should correspond to the main directions of the "4P-model" of modern medicine and represent an integral predictive, preventive and personalized system of examination and observation based on evidence-based medicine data and the requirements of practical obstetrics and perinatology.
Uma análise dos resultados dos diferentes tipos de escolas públicas no Sistema Permanente de Avaliação da Educação Básica do Ceará (Spaece)
Authors: Silva João Batista da , Duarte Patrícia Félix , Rebouças Aline De Oliveira , Vidal Eloísa Maia
Published: April 2021

O objetivo dessa pesquisa foi investigar a relação entre os diferentes tipos de escolas públicas cearenses de Ensino Médio e o desempenho dos alunos no Sistema Permanente de Avaliação da Educação Básica do Ceará (Spaece). Nesse sentido, foi realizada uma análise estatística descritiva com as notas de desempenho de 155 escolas do Ensino Médio da cidade de Fortaleza. Os resultados mostraram que as escolas de Educação Profissional tiveram melhor desempenho do que as Escolas Regulares e de Escolas de Tempo Integral, todavia, esses estão associados a complexidade de gestão da escola (ICG) e a quantidades de horas-aulas diária.
REVERBERAÇÕES DO PNAIC NA FORMAÇÃO DOCENTE DA ALFABETIZADORA
Authors: Lapa de Aguiar Maria Aparecida
Published: April 2021

O trabalho apresenta reflexões sobre a formação do Pacto Nacional pela Alfabetização na Idade Certa (PNAIC) e ancora-se em três questões:  quais são os impactos de uma formação que abrange o território nacional, com suas peculiaridades? Como essa formação se materializa na realidade de cada escola? Qual é a concepção de alfabetização defendida nesse processo complexo e difuso? Não se tenciona ter respostas fechadas para tais questões, entretanto elas nos auxiliam a pensar sobre a defesa do direito à formação continuada, que se constitui como tempo/espaço de tomada de consciência sobre o fazer pedagógico e sobre a compreensão do papel da escola como potencializadora da formação humana integral.
Mudanças ocorridas nos resultados da avaliação externa de escola com a transição de escolas não agrupadas para agrupamentos de escolas: o caso de duas organizações escolares
Authors: Albuquerque Pascoal , Gomes Ferreira António , Barreira Carlos
Published: April 2021

Visou-se investigar a influência da transição para agrupamentos de escolas nos resultados da Avaliação Externa de Escola (AEE) de duas organizações escolares situadas bem próximo geograficamente. Considerando os dois ciclos de AEE já finalizados em Portugal (2006-2011 e 2011-2017), efetuou-se uma análise documental (4 relatórios de AEE e 1 contraditório). Os resultados obtidos revelam, entre outros, uma melhoria da classificação no domínio “Resultados”. A passagem para agrupamento de escolas não influenciou negativamente as classificações obtidas por cada organização escolar.
GESTÃO DEMOCRÁTICA ESCOLAR À LUZ DAS IDÉIAS DE MICHEL DE CERTEAU
Authors: Queiroz Benedito José de , Medeiros Arilene Maria Soares de
Published: April 2021

Este artigo analisa como se materializa a gestão democrática no cotidiano de duas escolas públicas estaduais na cidade de Frutuoso Gomes-RN, à luz do aporte teórico de Michel de Certeau (2014). Pauta-se na abordagem qualitativa, com a utilização de observações diretas e registros no diário de pesquisa, conforme orienta Barbosa (2010). As práticas cotidianas revelam a verticalidade das relações e pouca participação nas deliberações tomadas nas escolas. Os embates nos processos de participação e decisão coletiva parecem assustar os sujeitos que praticam e articulam a gestão escolar.
A FORMAÇÃO DE COMPETÊNCIAS SOCIOEMOCIONAIS NA POLÍTICA NACIONAL DE ALFABETIZAÇÃO: UMA ANÁLISE CRÍTICA
Authors: Santos Jane Rigoldi dos , Vieira Juliana Piovesan , Futata Marli Delmonico de Araujo
Published: April 2021

O artigo discute a inclusão da formação socioemocional na Política Nacional de Alfabetização (PNA), expressa no decreto nº 9.765/2019, e sua vinculação com as propostas do Instituto Ayrton Senna (IAS), apresentadas no documento “Alfabetização 360º”. O objetivo é apresentar a concepção de alfabetização difundida pelo IAS e como esta representa a manutenção da precarização do processo de alfabetização no Brasil, ao trazer elementos secundários do processo de ensino e aprendizagem, como primários, neutralizando e dissolvendo a função da escola pública. Amparados pelo referencial teórico do materialismo histórico-dialético, entendemos que nenhuma prática de alfabetização é neutra, mas envolta por lutas, disputas de interesses, que tanto podem propor perspectivas de alfabetização que contribuam para a superação da sociedade capitalista, como também, de modo contraditório, como a “Alfabetização 360º”, podem propor a adaptação, ao reafirmar a formação de comportamentos amáveis, resilientes, condizentes com as necessidades do capital em um contexto de crise social e produtiva. Para desenvolver o artigo, realizamos uma pesquisa documental e bibliográfica de análise de documentos legislativos brasileiros (PNA), relatórios de organizações internacionais (UNESCO), folheto do IAS, artigos científicos e textos de autores da psicologia histórico-cultural. A conclusão a que chegamos é: está em curso um processo de reconfiguração do que entendemos ser alfabetização, direcionado pelo IAS, para o qual alfabetizar é mais do que ler e escrever textos, mas deter capacidades de leitura e regulação de emoções, pontuada como competência socioemocional. Há um processo de reconfiguração do conceito de alfabetização, que em nosso entendimento deve ser refutado.
ELEMENTOS SIMBÓLICOS DO CENTRO HISTÓRICO E DO NOVO CENTRO DE VITÓRIA - ES
Authors: Espindula Lidiane
Published: April 2021

A partir da segunda metade do século XIX grandes cidades no Brasil e o no mundo sofreram fortes intervenções em seu espaço urbano, modelado pelo capitalismo. Esse processo também pôde ser percebido na cidade de Vitória (ES), principalmente a partir da segunda metade do século XX, quando da transição do centro da cidade para outras regiões. Este artigo explana sobre as características dos antigos centros urbanos das grandes cidades, como também de Vitória, discutindo sobre os elementos arquitetônicos simbólicos, signos e linguagens característicos e os compara com os novos centros urbanos. A partir de pesquisas bibliográficas e visitas in loco para levantamento de dados das regiões estudadas, essa alegoria tem como objetivo compreender quais as simbologias do centro histórico da cidade e quais os novos elementos que vêm surgindo na transição desse espaço e que agora o configuram, ou seja, quais as novas imagens produzidas, seus signos e suas mensagens. Conclui-se que o centro histórico de Vitória possui valor afetivo e simbólico, porém há uma crescente valorização da imagem de uma paisagem homogeneizada característica do mundo moderno, encontrada no novo centro da cidade, com inexistência de um simbolismo particular, individual, diante de paisagens globalizadas.
A IMPORTÂNCIA DA BIOMASSA NA MATRIZ ENERGÉTICA BRASILEIRA
Authors: Silva Simão Pereira da , Costa Alexandre Sylvio Vieira da , Santos Sandro Luiz Barbosa dos , Laia Marcelo Luiz De
Published: April 2021

A biomassa compreende as matérias-primas renováveis da matéria orgânica de origem florestal, agrícola, ou de rejeitos urbanos e industriais, que pode se converter em energia sustentável pela combustão direta, por processos termoquímicos ou por processos biológicos. Na oferta interna de energia na matriz energética brasileira a participação de fontes renováveis é de 46,1%, nas quais se destacam a biomassa da cana (18%), a hidráulica (12,4%), a lenha e o carvão vegetal (8,7%) e outras renováveis (7%). Na exploração da biomassa brasileira para a geração de energia, destaca-se a cana de açucar na produção do etanol, o próprio bagaço da cana com outras biomassas na geração da bioeleletricidade, e as oleaginosas (principalmente a soja) na produção do biodiesel. Em 2019, foram produzidos 36,0 bilhões de litros de etanol, 25,3 bilhões de hidratado (aumento de 10%) e 10,7 bilhões de anidro (elevação de 16%) em relação a 2018. O bagaço de cana foi o combustível mais utilizado, com 82%, enquanto a participação de outras biomassas (licor negro, resíduos florestais, biogás, capim elefante, carvão vegetal, casca de arroz e gás de autoforno e lenha) foi de 18% na exportação de energia elétrica para o Sistema Interligado Nacional (SIN). Em 2019, foram consumidos 5,9 bilhões de litros de biodiesel no Brasil, o que representa um aumento de 11,3 % em relação a 2018. As pesquisas realizadas nas universidades brasileiras demonstram forte alinhamento com as perspectivas de aumento da exploração sustentável da biomassa brasileira, e com a busca por novas substâncias da biomassa através das atuais e de rotas tecnológicas inovadoras.