bluuebunny/crossref_metadata_2025 huggingface dataset:
Assessing the readiness for sustainable building material adoption: a study from construction organizations of different sizes
Authors: Nguyen Minh Van
Published: August 2024

Purpose

Sustainable building materials (SBMs) have recently been promoted to foster sustainable construction. While previous studies successfully presented the broad picture of SBM adoption, the question ‘What is the current state of SBM adoption in construction firms?’ was unanswered. Moreover, there is a lack of studies that investigate the impact of firm size on the adoption of SBMs. Therefore, this study aims to examine the level of readiness for SBM adoption in construction firms of different sizes.

Design/methodology/approach

Drawing on theoretical lenses and a review of previous studies, the study identified 20 factors affecting the readiness for SBM adoption. These factors were then grouped into market, organization, and employee readiness. Using the questionnaire survey, 229 valid responses were collected. The multiple pairwise comparison test showed significant differences in readiness for SBM adoption in firms of different sizes. The Fuzzy Synthetic Evaluation (FSE) analysis was then used to assess the level of readiness for SBM adoption in construction firms of different sizes.

Findings

The analysis of FSE showed that large-sized companies demonstrated the highest level of readiness for SBM adoption, followed by medium-sized and small-sized firms, respectively. These findings imply that larger construction firms may have more resources and organizational capacity to adopt SBMs. Conversely, smaller firms may require additional support or incentives to enhance their readiness for SBM adoption. It also highlights the need for targeted interventions and policies to promote SBM adoption across medium-sized and small-sized firms. Moreover, while market readiness emerged as the least critical category, the findings highlight the pivotal role of employee readiness across all firm sizes.

Originality/value

These findings underscore the importance of prioritizing employee readiness initiatives to facilitate the successful adoption of SBMs. Policymakers and industry stakeholders may need to focus on developing training programs and awareness campaigns tailored to employees, ensuring they are equipped with the necessary knowledge and skills. Moreover, strategies to enhance market readiness should also be explored through regulatory measures and incentives to encourage the adoption of SBMs. Additionally, fostering collaboration and networking among stakeholders can further promote market readiness and accelerate the uptake of SBMs in the construction industry.
The role of nostalgic emotion in shaping destination image and behavioral intentions – An empirical study
Authors: Nguyen Thi Thanh Thao , Duong Thi Dung Hanh
Published: August 2024

Purpose

Previous studies have proven the significant influence of nostalgia on tourists' perceptions, intentions, and behaviors. While much of the research has focused on nostalgia as a motivator for destination choice in the pre-trip stage, fewer studies have explored the effects of nostalgia experienced during visits on other behavioral intentions beyond revisit intention. This paper aims to fill this gap by examining the impact of nostalgic emotion experienced at tourist destinations on behavioral intentions, specifically pro-sustainable behavioral and word-of-mouth (WOM) intentions through destination image.

Design/methodology/approach

This study developed a conceptual model grounded in existing literature and empirical research on nostalgia and consumer behavior. Data were gathered from international tourists via surveys conducted in Hue City, a UNESCO World Heritage Site. The hypotheses were tested using structural equation modeling (SEM) with Amos 23.0.

Findings

The research results reveal that nostalgic emotion positively impacts destination image and WOM intention. Destination image correlates positively with both pro-sustainable behavioral intention and WOM intention. Destination image serves as a mediator in the relationship between nostalgic emotion and WOM intention. Theoretical and practical implications are also discussed in the current work.

Practical implications

The findings of this research provide guidance for nostalgic elements to be integrated into tourism experiences and marketing campaigns to enhance tourists' emotional connections, thereby improving the destination’s overall appeal. Additionally, while nostalgia does not directly foster pro-sustainable behavioral intentions, a positive destination image can influence these behaviors, suggesting that sustainable tourism strategies should also focus on strengthening the destination’s image.

Originality/value

This paper contributes to both theoretical advancements and practical applications in the field of tourism psychology and marketing by shifting the focus from nostalgia as a motivator for destination choice in the pre-trip stage to exploring the effects of nostalgic emotions experienced during visits on behavioral intentions beyond revisit intentions.
Contemporary management of ischemic priapism: A 12‐year population‐based analysis from a large US database
Authors: Licari Leslie Claire , Bologna Eugenio , Ditonno Francesco , Franco Antonio , Lasorsa Francesco , Bignante Gabriele , Proietti Flavia , Leonardo Costantino , Anele Uzoma A. , Cherullo Edward E. , Levine Laurence A. , Autorino Riccardo , Manfredi Celeste
Published: August 2024

Background

Priapism is a urological condition characterized by a persistent erection. The management varies based on its subclassifications. Despite established clinical guidelines for ischemic priapism, there is a lack of large‐scale research focused on patient characteristics and management strategies.

Objectives

To analyze the contemporary management of ischemic priapism in the US, exploring patient demographics and clinical characteristics, as well as predictors of erectile dysfunction (ED) and penile prosthesis implantation (PPI).

Materials and methods

We performed a retrospective analysis of the PearlDiver Mariner database, reviewing records from 2010–2021. Adult males diagnosed with ischemic priapism were included. Data analysis covered demographic, clinical variables, and management strategies. Predictors of de novo ED and PPI were evaluated using multivariable logistic regression analysis.

Results

Of 36,120 patients, most (93%) received only medical management, and a minority underwent surgical interventions (penile shunt surgery [PSS], PPI or both). Medical management was typically effective, as 67.08% of the patients in this group experienced only one episode of priapism. However, de novo ED occurred in 16.57% of these patients. The majority of patients undergoing PPI had an inflatable prosthesis (81%). Older age (odds ratio, OR 1.02), the presence of metabolic diseases (OR 1.39), neurogenic disorders (OR 1.72), solid pelvic malignancies (OR 1.09), and multiple episodes of priapism were identified as significant predictors of de novo ED (all p < 0.05). Similarly, age (OR 1.03), the presence of metabolic diseases (OR 1.23), solid pelvic malignancies (OR 1.99), and multiple episodes of priapism were associated with higher likelihood of PPI (all p < 0.05).

Conclusion

Most cases of ischemic priapism are managed with the medical therapy. Less than 3% of patients with ischemic priapism receive PPI, and when this occurs an inflatable prosthesis is favored. Age, specific comorbidities, and multiple episodes of priapism appear to be significant predictors of ED and PPI.
Sports Cages as Social Infrastructure: Sociality, Context, and Contest in Hackney's Cages
Authors: Billingham Luke , Curry Fraser , Crossley Stephen
Published: August 2024

The concept of social infrastructure has experienced a rapid rise to prominence in recent years, both in academia and in policy. In this article, we explore a case study of cages (also known as Multi‐Use Games Areas) in Hackney, North‐East London. We argue that cages are forms of urban infrastructure which can facilitate multiple forms of sociality—especially for young people—and can thus be deemed valuable social infrastructure. However, this value can only be understood in context—in relation to the joys and harms of growing up in Hackney—and as in contest—the status and meaning of the cage is different for different groups, and there are considerable tensions over their use, ownership, and management. In our examination of the cage, we aim to explore and build upon existing conceptions of social infrastructure.
NL13, a novel curcumin analogue and polo like kinase 4 inhibitor, induces cell cycle arrest and apoptosis in prostate cancer models
Authors: Qiao Xinyi , Zheng Ke , Ye Lei , Yang Jin , Cui Rong , Shan Yuanyuan , Li Xiaoheng , Li Huitao , Zhu Qiqi , Zhao Zhiguang , Ge Ren‐shan , Wang Yiyan
Published: August 2024

Background and Purpose

Prostate cancer remains a major public health burden worldwide. Polo like kinase 4 (PLK4) has emerged as a promising therapeutic target in prostate cancer due to its key roles in cell cycle regulation and tumour progression. This study aims to develop and characterize the novel curcumin analogue NL13 as a potential therapeutic agent and PLK4 inhibitor against prostate cancer.

Experimental Approach

NL13 was synthesized and its effects were evaluated in prostate cancer cells and mouse xenograft models. Kinome screening and molecular modelling identified PLK4 as the primary target. Antiproliferative and proapoptotic mechanisms were explored via cell cycle, apoptosis, gene and protein analyses.

Key Results

Compared with curcumin, NL13 exhibited much greater potency in inhibiting PC3 (IC50, 3.51 μM vs. 35.45 μM) and DU145 (IC50, 2.53 μM vs. 29.35 μM) prostate cancer cells viability and PLK4 kinase activity (2.32 μM vs. 246.88 μM). NL13 induced G2/M cell cycle arrest through CCNB1/CDK1 down‐regulation and triggered apoptosis via caspase‐9/caspase‐3 cleavage. These effects were mediated by PLK4 inhibition, which led to the inactivation of the AKT signalling pathway. In mice, NL13 significantly inhibited tumour growth and modulated molecular markers consistent with in vitro findings, including decreased p‐AKT and increased cleaved caspase‐9/3.

Conclusion and Implications

NL13, a novel PLK4‐targeted curcumin analogue, exerts promising anticancer properties against prostate cancer by disrupting the PLK4‐AKT‐CCNB1/CDK1 and apoptosis pathways. NL13 represents a promising new agent for prostate cancer therapy.
The Executive Bully Pulpit: Drivers of CEO Sociopolitical Activism in the Wake of Social Movements
Authors: Acharya Keshab , Abebe Michael A. , Kroll Mark , Solano Guadalupe
Published: August 2024

Research Question/Issue

CEO sociopolitical activism seems to be on the rise in response to prominent social movements around social justice (e.g., Black Lives Matter), gender equality (e.g., #MeToo movement), and climate change (e.g., People's Climate Movement), to name a few. Engaging in such activism could be risky for executives, given its potential to appease some stakeholders while alienating others. This begs the question: Why do some CEOs become sociopolitical activists while others remain on the “sidelines”? This study addresses this question by exploring the notion of the executive “bully pulpit” and how CEOs leverage their status and reputation to publicly engage in sociopolitical activism.

Research Findings/Insights

Drawing from the status and reputation literature, we explore the effect of CEO power, CEO celebrity status, and firm reputation as predictors of CEO sociopolitical activism. In doing so, we focus on the “bully pulpit” explanation by arguing that powerful, high‐status CEOs and those who lead firms with good reputations are more likely to use their professional position and visibility to advocate for or against controversial sociopolitical issues. Our analysis of CEO sociopolitical activism data from 246 matched‐pair S&P 500 firms from 2007 to 2020 largely supports our arguments, though we find there is an important interaction between firm reputation and both CEO power and celebrity.

Theoretical/Academic Implications

This study extends current research insights by highlighting how power, status, and reputation at the CEO and firm levels create a formidable platform (a “bully pulpit”) from which executive sociopolitical activism is exercised. Given its nascent nature, scholars are just beginning to empirically explore the consequences of CEO sociopolitical activism. This study contributes to ongoing work in this area by providing empirical evidence on the nature and drivers of CEO sociopolitical activism.

Practitioner/Policy Implications

Firms seeking to play a more proactive role in contemporary sociopolitical issues need to consider hiring high‐profile CEOs. Additionally, highly reputed firms are well‐positioned to support their CEOs' efforts in influencing societal debates on controversial issues.
Exploring transformer models for sentiment classification: A comparison of BERT, RoBERTa, ALBERT, DistilBERT, and XLNet
Authors: Areshey Ali , Mathkour Hassan
Published: August 2024

Transfer learning models have proven superior to classical machine learning approaches in various text classification tasks, such as sentiment analysis, question answering, news categorization, and natural language inference. Recently, these models have shown exceptional results in natural language understanding (NLU). Advanced attention‐based language models like BERT and XLNet excel at handling complex tasks across diverse contexts. However, they encounter difficulties when applied to specific domains. Platforms like Facebook, characterized by continually evolving casual and sophisticated language, demand meticulous context analysis even from human users. The literature has proposed numerous solutions using statistical and machine learning techniques to predict the sentiment (positive or negative) of online customer reviews, but most of them rely on various business, review, and reviewer features, which leads to generalizability issues. Furthermore, there have been very few studies investigating the effectiveness of state‐of‐the‐art pre‐trained language models for sentiment classification in reviews. Therefore, this study aims to assess the effectiveness of BERT, RoBERTa, ALBERT, DistilBERT, and XLNet in sentiment classification using the Yelp reviews dataset. The models were fine‐tuned, and the results obtained with the same hyperparameters are as follows: 98.30 for RoBERTa, 98.20 for XLNet, 97.40 for BERT, 97.20 for ALBERT, and 96.00 for DistilBERT.
Fatigue analysis of spherulitic semi‐crystalline polymers: Unveiling the effects of microstructure and defect
Authors: Jiang Chenxu , Zhou Jia , Cui Jiaxin , Shi Jingfu , Miao Changqing
Published: August 2024

A micromechanical model considering the spherulite structure of semi‐crystalline polymers was established in this study. The micro stress–strain histories were captured by combining the constitutive equations and multi‐axial fatigue criterion. The continuous damage theory was employed to describe the degradation of material properties during cycle loading. Based on the proposed model, the effects of microstructure features, such as grain anisotropy, defects, and crystallinity, on the fatigue performance was examined under multi‐axial loading condition. The local material degradation and damage accumulation were then focused on to understand the underlying fatigue mechanisms with various microstructures. Meanwhile, the crack initiation site was precisely predicted and discussed. This research provides theoretical support for understanding the failure mechanisms of spherulitic semi‐crystalline polymers, deepening the understanding of associated microstructural characteristics and strengthening the anti‐fatigue design of semi‐crystalline polymers.
Impact of the new government‐involved noninvasive prenatal testing certification system on the awareness of pregnant women about noninvasive prenatal testing in Japan
Authors: Shirato Nahoko , Sekizawa Akihiko , Miyagami Keiko , Sakamoto Miwa , Yamada Takahiro , Hirose Tatsuko , Ikebukuro Shin , Nakamura Takeshi , Mizutani Akane , Ikemoto Mai , Izum Mikiko , Seino Hitomi , Yamada Shigehito , Suzumori Nobuhiro , Yoshihashi Hiroshi , Samura Osamu , Sawai Hideaki , Sago Haruhiko , Okuyama Torayuki
Published: August 2024

Aim

In Japan, noninvasive prenatal testing (NIPT) has been performed by facilities accredited by the Japanese Society of Obstetrics and Gynecology since 2013. However, since 2016, with the implementation of NIPT, which can only be performed by blood sampling, non‐obstetricians have been involved in prenatal testing. Therefore, in July 2022, a new government‐involved NIPT certification system based on Health Sciences Council guidelines was introduced to ensure access to prenatal testing information for pregnant women.

Methods

This survey was conducted in February 2023 and was the first survey after the certification system implementation. We conducted a web‐based survey of 1227 pregnant women and nursing mothers who underwent NIPT after July 2022 to evaluate their experiences.

Results

Respondents were categorized by certification status as certified (C: 56%), non‐certified (non‐C: 23%), or uncertain (Q: 20%). The C group with a higher mean age at examination (35.0 ± 4.5 years) paid lower examination fees, received longer pre‐ and post‐examination explanations, and underwent more weekday examinations (80%) than the other groups. Most respondents, 67%, 48%, and 53% in the C, non‐C, and Q groups, respectively (p < 0.0001), stated that “NIPT needs to be regulated by the government or academic societies.” The non‐C group was more likely to say, “Insufficient post‐test explanations at the laboratory made me more anxious,” than the other groups when the testing results were non‐negative (p = 0.015).

Conclusions

Despite government regulation, some pregnant women choose convenience over certified facilities, risking inadequate care. The government should ensure that NIPT is a safe option for all pregnant women.
Looking into the black mirror of the overdose crisis: Assessing the harms of collaborative surveillance technologies in the United States response
Authors: Syvertsen Jennifer
Published: August 2024

Drug overdose is a leading cause of death among adults in the United States, prompting calls for more surveillance data and data sharing across public health and law enforcement to address the crisis. This paper integrates Black feminist science and technology studies (STS) into an anthropological analysis of the collision of public health, policing, and technology as embedded in the US National Overdose Response Strategy and its technological innovation, the Overdose Detection Mapping Application Program (ODMAP). The dystopian Netflix series “Black Mirror,” which explores the seemingly useful but quietly destructive potential of technology, offers a lens through which to speculate upon and anticipate the harms of collaborative surveillance projects. Ultimately, I ask: are such technological interventions a benevolent approach to a public health crisis or are we looking into a black mirror of racialized surveillance and criminalization of overdose in the United States?
“Use what you have: ” Health promotion and economic vitality in a COVID‐19 worksite vaccination initiative
Authors: McClure Stephanie M. , Oths Kathryn , Foster Pamela Payne , Radcliffe Olivia R. , Lichtenstein Bronwen
Published: August 2024

In mid‐2021, the CommuniVax Alabama team broadened their community engagement by partnering with the Chamber of Commerce, the Alabama Department of Public Health, and others to bring vaccines to the workplace. Through this collaboration, the team hoped to fortify the state's persistently anemic rate of vaccination. The goals of increasing access to, and convenience of COVID‐19 vaccination were only partially achieved due to human resource and policy challenges and incongruent priorities that resulted from those challenges. The accomplishments, challenges, and suggestions for improvement are outlined to aid future efforts at health promotion and community outreach, especially in jurisdictions with limited extant public health infrastructure.
Gradual Transverse Transport of Naturally Tibialized Fibula Using Hexapod Frames to Treat Neglected Type V Tibial Segmental Defects
Authors: Du Hui , Li Heng , He Xiaotian , Wu Yong
Published: August 2024

Objective

Although several reconstructive methods have been developed to manage large segmental tibial bone defects including bone transport (distraction osteogenesis), contralateral fibular graft, allograft, tibiofibular synostosis, Masquelet technique, and 3D printed scaffold, neglected large tibial defects in adults remain challenging problems. This study describes gradual transverse transport of naturally tibialized fibula using hexapod frames in management of adult patients with neglected large tibial defects.

Methods

We retrospectively reviewed four cases of transverse transport of naturally tibialized fibula from November 2018 to February 2022. We measured the length of the tibial defect and the transported fibular segment, the mid‐diaphyseal diameter and cortical thickness of the affected fibula, contralateral fibula, and tibia. The parameters measured both preoperatively and postoperatively were leg length discrepancy, hip‐knee‐ankle angle, medial proximal tibial angle, posterior proximal tibial angle, lateral distal tibial angle, range of motion of the knee and ankle joints, and Lower Extremity Functional Scores (LEFS). Patients' satisfaction rates using Likert scale were also recorded.

Results

Among four female patients, three suffered from tibial osteomyelitis, and one was due to congenital pseudarthrosis of the tibia. The average follow‐up time was 2.7 ± 1.4 years. The average length of tibial defect was 14.0 ± 0.8 cm. The average preoperative shortening of the affected leg was 9.0 ± 2.5 cm, which changed to 0.6 ± 0.8 cm postoperatively. The median length of the transported fibular segment was 15.2 cm. Two patients had varus deformity, two had recurvatum, and one had procurvatum preoperatively. Postoperative radiological measurement showed all deformities corrected and no ankle valgus deformity developed during follow‐up. All patients achieved union and can fully weight bear on the affected extremity. The average fixator time was 12.9 ± 2.9 months. The average preoperative and postoperative LEFS, respectively, were 53.5 ± 5.0, 70.5 ± 1.3, with a significant difference (p = 0.003). Three patients reported very satisfied with the outcome, and one patient reported satisfied. Three patients had pin tract infections, and one patient had skin necrosis which healed after additional surgery. One patient had surgical release of the hamstring tendons due to flexion contracture of the knee. Two patients had 15° of reduction in ankle range of motion. One patient had transient common peroneal nerve palsy which spontaneously recovered within 6 weeks.

Conclusion

The transverse transport of naturally tibialized fibula was both a safe and effective method to treat the long‐standing type V tibial segmental defect.
Loss‐of‐function of LIGULELESS1 activates the jasmonate pathway and promotes maize resistance to corn leaf aphids
Authors: Huang Shijie , Wang Chuanhong , Wang Ling , Li Shuai , Wang Tengyue , Tao Zhen , Zhao Yibing , Ma Jing , Zhao Mengjie , Zhang Xinqiao , Wang Lei , Xie Chuanxiao , Li Peijin
Published: August 2024

Corn leaf aphids (Rhopalosiphum maidis) are highly destructive pests of maize (Zea mays) that threaten growth and seed yield, but resources for aphid resistance are scarce. Here, we identified an aphid‐resistant maize mutant, resistance to aphids 1 (rta1), which is allelic to LIGULELESS1 (LG1). We confirmed LG1's role in aphid resistance using the independent allele lg1‐2, allelism tests and LG1 overexpression lines. LG1 interacts with, and increases the stability of ZINC‐FINGER PROTEIN EXPRESSED IN INFLORESCENCE MERISTEM (ZIM1), a central component of the jasmonic acid (JA) signalling pathway, by disturbing its interaction with the F‐box protein CORONATINE INSENSITIVE 1a (COI1a). Natural variation in the LG1 promoter was associated with aphid resistance among inbred lines. Moreover, a loss‐of‐function mutant in the LG1‐related gene SPL8 in the dicot Arabidopsis thaliana conferred aphid resistance. This study revealed the aphid resistance mechanism of lg1, providing a theoretical basis and germplasm for breeding aphid‐resistant crops.
The contribution of intimate partner violence to socioeconomic inequality among Black, Latina, and White women
Authors: Kaufman Joanne M.
Published: August 2024

Women on average make less money than men, and this is particularly acute for Black and Latina women. While there are several contributors to socioeconomic inequality, violent victimization coming at critical times (adolescence, early adulthood) is one such contributor. Intimate partner violence (IPV) particularly impacts women, with Black and Latina women facing higher levels. Thus, it is important to consider for understanding inequality. This research examined the long‐term impact of IPV in adolescence and early adulthood on educational attainment and income among Black, Latina, and White women using the Add Health data. I found strong evidence for the impact of early adult IPV on reducing educational attainment among all women 15 years later and evidence for related reductions in income for Latina and White women. In addition, I found evidence that mental and physical health along with school attendance mediated the impact of IPV on later socioeconomic outcomes for Latina and White women. However, there was no evidence for the mediating mechanisms for Black women. Overall, this research provides evidence for the long‐term effects of IPV on socioeconomic inequality among women and highlights how the effects may differ by race and ethnicity.
Acute exposure to carbon monoxide inhalation and/or hot water immersion transiently increases erythropoietin in females but not in males
Authors: DiMarco Kaitlyn G. , Chapman Christopher L. , Weiser Natasha E. , Matsell Emma R. , Lucernoni Kathryn M. , Chacon Samantha , Grivette Margaret M. B. , Halliwill John R. , Lovering Andrew T. , Minson Christopher T.
Published: August 2024

The use of acute carbon monoxide inhalation (COi) and hot water immersion (HWI) are of growing interest as interventions to stimulate erythropoietin (EPO) production. However, whether EPO production is further augmented when combining these stressors and whether there are sex differences in this response are poorly understood. Therefore, we measured circulating EPO concentration in response to acute COi and HWI independently and in combination and determined whether the responses were altered by sex. Participants completed three study visits—COi, HWI, and combined COi and HWI—separated by 1 week in a randomized, balanced, crossover design. Renal blood velocity was measured during all interventions, and carboxyhaemoglobin was measured during and after COi. Serum samples were analysed every hour for 6 h post‐intervention for EPO concentration. HWI decreased renal blood velocity (46.2 cm/s to 36.2 cm/s) (P < 0.0001), and COi increased carboxyhaemoglobin (1.5%–12.8%) (P < 0.0001) without changing renal blood velocity (46.4–45.2 cm/s) (P = 0.4456). All three interventions increased peak EPO concentration from baseline (COi: 6.02–9.74 mIU/mL; HWI: 6.80–11.10 mIU/mL; COi + HWI: 6.71–10.91 mIU/mL) (P = 0.0048) and to the same extent (P = 0.3505). On average, females increased EPO while males did not in response to COi (females: 6.17 mIU/mL; males: 1.27 mIU/mL) (P = 0.0010), HWI (females: 6.47 mIU/mL; males: 2.14 mIU/mL) (P = 0.0104), and COi and HWI (females: 6.65 mIU/mL; males: 1.76 mIU/mL) (P = 0.0256). These data emphasize that combining these interventions does not augment EPO secretion and that these interventions may work better in females.
Hebbian instruction of axonal connectivity by endogenous correlated spontaneous activity
Authors: Matsumoto Naoyuki , Barson Daniel , Liang Liang , Crair Michael C.
Published: August 2024

Spontaneous activity refines neural connectivity prior to the onset of sensory experience, but it remains unclear how such activity instructs axonal connectivity with subcellular precision. We simultaneously measured spontaneous retinal waves and the activity of individual retinocollicular axons and tracked morphological changes in axonal arbors across hours in vivo in neonatal mice. We demonstrate that the correlation of an axon branch’s activity with neighboring axons or postsynaptic neurons predicts whether the branch will be added, stabilized, or eliminated. Desynchronizing individual axons from their local networks, changing the pattern of correlated activity, or blocking
N
-methyl-
d
-aspartate receptors all significantly altered single-axon morphology. These observations provide the first direct evidence in vivo that endogenous patterns of correlated neuronal activity instruct fine-scale refinement of axonal processes.
Divergent recruitment of developmentally defined neuronal ensembles supports memory dynamics
Authors: Kveim Vilde A. , Salm Laurenz , Ulmer Talia , Lahr Maria , Kandler Steffen , Imhof Fabia , Donato Flavio
Published: August 2024

Memories are dynamic constructs whose properties change with time and experience. The biological mechanisms underpinning these dynamics remain elusive, particularly concerning how shifts in the composition of memory-encoding neuronal ensembles influence the evolution of a memory over time. By targeting developmentally distinct subpopulations of principal neurons, we discovered that memory encoding resulted in the concurrent establishment of multiple memory traces in the mouse hippocampus. Two of these traces were instantiated in subpopulations of early- and late-born neurons and followed distinct reactivation trajectories after encoding. The divergent recruitment of these subpopulations underpinned gradual reorganization of memory ensembles and modulated memory persistence and plasticity across multiple learning episodes. Thus, our findings reveal profound and intricate relationships between ensemble dynamics and the progression of memories over time.
A hippocampal circuit mechanism to balance memory reactivation during sleep
Authors: Karaba Lindsay A. , Robinson Heath L. , Harvey Ryan E. , Chen Weiwei , Fernandez-Ruiz Antonio , Oliva Azahara
Published: August 2024

Memory consolidation involves the synchronous reactivation of hippocampal cells active during recent experience in sleep sharp-wave ripples (SWRs). How this increase in firing rates and synchrony after learning is counterbalanced to preserve network stability is not understood. We discovered a network event generated by an intrahippocampal circuit formed by a subset of CA2 pyramidal cells to cholecystokinin-expressing (CCK+) basket cells, which fire a barrage of action potentials (“BARR”) during non–rapid eye movement sleep. CA1 neurons and assemblies that increased their activity during learning were reactivated during SWRs but inhibited during BARRs. The initial increase in reactivation during SWRs returned to baseline through sleep. This trend was abolished by silencing CCK+ basket cells during BARRs, resulting in higher synchrony of CA1 assemblies and impaired memory consolidation.
A BARRage of firing while asleep
Authors: Mou Xiang , Ji Daoyun
Published: August 2024

Memory reactivation requires counterbalancing to consolidate memories
In Science Journals
Authors: Fogg Christiana N. , Simonti Corinne N. , Lavine Marc S. , Smith Keith T. , Stern Peter , Suleymanov Yury , Stajic Jelena , Ogle Molly , Maroso Mattia , Jiang Di , Ash Caroline , Funk Michael A. , Grocholski Brent , Yeston Jake S. , Smith Jesse , VanHook Annalisa M.
Published: August 2024

Highlights from the
Science
family of journals
New results intensify debate over cosmic expansion rate
Authors: Clery Daniel
Published: August 2024

Triple measurement of the Hubble constant using JWST suggests universe doesn’t need “new physics”
What’s next for psychedelics after MDMA rejection?
Authors: Kupferschmidt Kai
Published: August 2024

Lykos’s failure to win FDA approval for PTSD therapy may accelerate a strategic shift among its competitors
Supplemental Material: Don’t mind the “charcoal gap”: A reassessment of Devonian wildfire
Authors: Glasspool Ian J. , Gastaldo Robert A.
Published: August 2024

Charcoal and inertinite locality and quantification data.  
Reaction of 2,3,4,5,6-Pentafluorobenzamide with Potassium Hydride: Unexpected Activation of the C–F Bond and Dimerization of Organofluorine Ligand
Authors: Yambulatov D. S. , Astaf’eva T. V. , Voronina J. K. , Nikolaevskii S. A. , Kiskin M. A. , Eremenko I. L.
Published: August 2024

The reaction of potassium hydride with 2,3,4,5,6-pentafluorobenzamide (FBAm) in dimethoxyethane results in activation of the C–F bond in the para-position to the C(O)NH2 group followed by dimerization of FBAm to form a potassium salt with one free amide group. The structure of the binuclear reaction product {(DME)2K+[C6F5–C(O)N–C6F4–C(O)NH2]–}2 (I) was determined by X-ray diffraction (CCDC 2311402), the purity of the product was confirmed by NMR spectroscopy.
Classifying intrusive and strongly metamorphosed rock units: CLASS—a cooperative lithodemic and stratigraphic system
Authors: Maxeiner Ralf O. , Bosman Sean A. , Card Colin D. , Marsh Arden , Morelli Ryan M. , Couëslan Chris , Martins Tania , Reid Kyle , Easton R. Michael , Knox Bernadette , Mihalynuk Mitchell G. , Ootes Luke , Cui Yao , Grobe Matthias , Guemache Mehdi A. , Lawley Christopher J.M. , Böhm Chris , Ashton Ken E.
Published: August 2024

We review currently available Canadian and international lithostratigraphic and lithodemic schemes and find most of them inadequate for classifying intrusive and strongly metamorphosed rocks of Canada and beyond. A new system is proposed, one that unifies, complements, and extends components of the revised North American Stratigraphic Code, the British Geological Survey Rock Unit Classification System of 2021, and the International Stratigraphic Guide of 1999. This new Cooperative Lithodemic and Stratigraphic System (CLASS) is intended to serve as a practical guide to geoscientists who need to classify and report on lithodemic units in North America and has broad applicability to other jurisdictions. It is built with database management practices in mind and employs the concept of inheritance of root characteristics between seven formal rock unit ranks, which will allow incorporation of the scheme into compact object-oriented and relational databases designed for purpose. Broad application of CLASS, especially to historically problematic lithodemic units, should help to foster jurisdictional interoperability, data sharing, global compilations, and thematic studies. At the heart of this proposed system is a subdivision and accompanying nomenclature that allows classification of rocks into seven formal ranks. Following the well-established lithostratigraphic supergroup, group, subgroup, formation, member, submember, and bed approach, CLASS proposes three classes of lithodemic subdivisions for formally naming lithodemic rock units.
The Molecular Substrates of Insect Eusociality
Authors: Kocher Sarah , Kingwell Callum
Published: August 2024

The evolution of eusociality in Hymenoptera—encompassing bees, ants, and wasps—is characterized by multiple gains and losses of social living, making this group a prime model to understand the mechanisms that underlie social behavior and social complexity. Our review synthesizes insights into the evolutionary history and molecular basis of eusociality. We examine new evidence for key evolutionary hypotheses and molecular pathways that regulate social behaviors, highlighting convergent evolution on a shared molecular toolkit that includes the insulin/insulin-like growth factor signaling (IIS) and target of rapamycin (TOR) pathways, juvenile hormone and ecdysteroid signaling, and epigenetic regulation. We emphasize how the crosstalk among these nutrient-sensing and endocrine signaling pathways enables social insects to integrate external environmental stimuli, including social cues, with internal physiology and behavior. We argue that examining these pathways as an integrated regulatory circuit and exploring how the regulatory architecture of this circuit evolves alongside eusociality can open the door to understanding the origin of the complex life histories and behaviors of this group.
Supplementary Table 5 from Transient Differentiation-State Plasticity Occurs during Acute Lymphoblastic Leukemia Initiation
Authors: Poort Vera M. , Hagelaar Rico , van Roosmalen Markus J. , Trabut Laurianne , Buijs-Gladdines Jessica G. C. A. M. , van Wijk Bram , Meijerink Jules , van Boxtel Ruben
Published: August 2024

Table 5: TCR alignments
Supplementary Figures from Transient Differentiation-State Plasticity Occurs during Acute Lymphoblastic Leukemia Initiation
Authors: Poort Vera M. , Hagelaar Rico , van Roosmalen Markus J. , Trabut Laurianne , Buijs-Gladdines Jessica G. C. A. M. , van Wijk Bram , Meijerink Jules , van Boxtel Ruben
Published: August 2024

Supplementary Figures
Figure S5 from Serine Depletion Promotes Antitumor Immunity by Activating Mitochondrial DNA-Mediated cGAS-STING Signaling
Authors: Saha Suchandrima , Ghosh Monisankar , Li Jinyu , Wen Asher , Galluzzi Lorenzo , Martinez Luis A. , Montrose David C.
Published: August 2024

Figure S5
Supplementary Table S1 from Targeting Super-Enhancer–Driven Transcriptional Dependencies Suppresses Aberrant Hedgehog Pathway Activation and Overcomes Smoothened Inhibitor Resistance
Authors: Sui Yi , Wang Teng , Mei Yanqing , Zhu Ying , Jiang Wenyan , Shen Jiayi , Yan Siyuan , Lu Wenjie , Zhao Kewen , Mo Jialin , Wang Chaochen , Tang Yujie
Published: August 2024

Supplementary Table S1. PCR primers for NGS. The primer sequences for amplifying sgRNA sequences from genome DNA for NGS assays.
Figure S12 from Serine Depletion Promotes Antitumor Immunity by Activating Mitochondrial DNA-Mediated cGAS-STING Signaling
Authors: Saha Suchandrima , Ghosh Monisankar , Li Jinyu , Wen Asher , Galluzzi Lorenzo , Martinez Luis A. , Montrose David C.
Published: August 2024

Figure S12
Supplementary Figures S1-S15 and Figure Legends and Table Legends from Targeting Super-Enhancer–Driven Transcriptional Dependencies Suppresses Aberrant Hedgehog Pathway Activation and Overcomes Smoothened Inhibitor Resistance
Authors: Sui Yi , Wang Teng , Mei Yanqing , Zhu Ying , Jiang Wenyan , Shen Jiayi , Yan Siyuan , Lu Wenjie , Zhao Kewen , Mo Jialin , Wang Chaochen , Tang Yujie
Published: August 2024

The PDF file includes Supplementary Figures S1-S15, Figure Legends, and Table Legends
Figure S11 from Serine Depletion Promotes Antitumor Immunity by Activating Mitochondrial DNA-Mediated cGAS-STING Signaling
Authors: Saha Suchandrima , Ghosh Monisankar , Li Jinyu , Wen Asher , Galluzzi Lorenzo , Martinez Luis A. , Montrose David C.
Published: August 2024

Figure S11
Supplementary Data from Increased Protein Kinase A Activity Induces Fibrolamellar Hepatocellular Carcinoma Features Independent of DNAJB1
Authors: Shirani Mahsa , Levin Solomon , Shebl Bassem , Requena David , Finkelstein Tova M. , Johnson Daniel S. , Ng Denise , Lalazar Gadi , Heissel Søren , Hojrup Peter , Molina Henrik , de Jong Ype P. , Rice Charles M. , Singhi Aatur D. , Torbenson Michael S. , Coffino Philip , Lyons Barbara , Simon Sanford M.
Published: August 2024

Supplemental Table 1 Levels of PKA subunits between FLC and adjacent normal tissue as assessed by mass spectrometry (peptides) or transcriptome. With trypsin digestion a unique peptide was not detected at the fusion junction between DNAJB1 and PRKACA
Supplementary Data 2 from Increased Protein Kinase A Activity Induces Fibrolamellar Hepatocellular Carcinoma Features Independent of DNAJB1
Authors: Shirani Mahsa , Levin Solomon , Shebl Bassem , Requena David , Finkelstein Tova M. , Johnson Daniel S. , Ng Denise , Lalazar Gadi , Heissel Søren , Hojrup Peter , Molina Henrik , de Jong Ype P. , Rice Charles M. , Singhi Aatur D. , Torbenson Michael S. , Coffino Philip , Lyons Barbara , Simon Sanford M.
Published: August 2024

Supplemental Table 2: EC50 (µm) of the drugs tested on cells dissociated from patients tumors expressing (middle column) DNAJB1::PRKACA or (right column) ATP1B1::PRKACA.
Data from Targeting Super-Enhancer–Driven Transcriptional Dependencies Suppresses Aberrant Hedgehog Pathway Activation and Overcomes Smoothened Inhibitor Resistance
Authors: Sui Yi , Wang Teng , Mei Yanqing , Zhu Ying , Jiang Wenyan , Shen Jiayi , Yan Siyuan , Lu Wenjie , Zhao Kewen , Mo Jialin , Wang Chaochen , Tang Yujie
Published: August 2024

Abstract
Aberrant activation of the Hedgehog (Hh) signaling pathway plays important roles in oncogenesis and therapeutic resistance in several types of cancer. The clinical application of FDA-approved Hh-targeted smoothened inhibitors (SMOi) is hindered by the emergence of primary or acquired drug resistance. Epigenetic and transcriptional-targeted therapies represent a promising direction for developing improved anti-Hh therapies. In this study, we integrated epigenetic/transcriptional-targeted small-molecule library screening with CRISPR/Cas9 knockout library screening and identified CDK9 and CDK12, two transcription elongation regulators, as therapeutic targets for antagonizing aberrant Hh activation and overcoming SMOi resistance. Inhibition of CDK9 or CDK12 potently suppressed Hh signaling and tumor growth in various SMOi responsive or resistant Hh-driven tumor models. Systemic epigenomic profiling elucidated the Hh-driven super-enhancer (SE) landscape and identified IRS1, encoding a critical component and cytoplasmic adaptor protein of the insulin-like growth factor (IGF) pathway, as an oncogenic Hh-driven SE target gene and effective therapeutic target in Hh-driven tumor models. Collectively, this study identifies SE-driven transcriptional dependencies that represent promising therapeutic vulnerabilities for suppressing the Hh pathway and overcoming SMOi resistance. As CDK9 and IRS inhibitors have already entered human clinical trials for cancer treatment, these findings provide comprehensive preclinical support for developing trials for Hh-driven cancers.

Significance: Dissecting transcriptional dependencies driven by super-enhancers uncovers therapeutic targets in Hedgehog-driven cancers and identifies strategies for overcoming resistance to smoothened inhibitors.
Supplementary Figure S2 from Urinary Metabolite Diagnostic and Prognostic Liquid Biopsy Biomarkers of Lung Cancer in Nonsmokers and Tobacco Smokers
Authors: Dalal Bhavik , Tada Takeshi , Patel Daxesh P. , Pine Sharon R. , Khan Mohammed , Oike Takahiro , Kanke Yasuyuki , Parker Amelia L. , Haznadar Majda , Toulabi Leila , Krausz Kristopher W. , Robles Ana I. , Bowman Elise D. , Gonzalez Frank J. , Harris Curtis C.
Published: August 2024

Distribution of CR and NANA metabolite levels in (A) Exploratory cohort and (B) Validation cohort adenocarcinoma cases. ****p<0.0001; LUAD, lung adenocarcinoma; x͂ = median
Supplementary Table S2 from Intraperitoneal Nivolumab after Debulking Surgery and Hyperthermic Intraperitoneal Chemotherapy in Advanced Ovarian Cancer: A Phase I Study with Expansion Cohort
Authors: Corbaux Pauline , Freyer Gilles , Glehen Olivier , You Benoît , Bakrin Naoual , Gelot Audrey , Dayde David , Sajous Christophe , Piffoux Max , Péron Julien , Lescuyer Gaelle , Payen Lea , Kepenekian Vahan
Published: August 2024

Summary of adverse events according to their attributed relationship
Supplementary Material S1 from Tissue-Free Liquid Biopsies Combining Genomic and Methylation Signals for Minimal Residual Disease Detection in Patients with Early Colorectal Cancer from the UK TRACC Part B Study
Authors: Slater Susanna , Bryant Annette , Aresu Maria , Begum Ruwaida , Chen Hsiang-Chi , Peckitt Clare , Lazaro-Alcausi Retchel , Carter Paul , Anandappa Gayathri , Khakoo Shelize , Melcher Lucinda , Potter Vanessa , Marti Francisca M. , Huang Joesph , Branagan Graham , George Nicol , Abulafi Muti , Duff Sarah , Raja Ashraf , Gupta Ashish , West Nicholas , Bucheit Leslie , Rich Thereasa , Chau Ian , Cunningham David , Starling Naureen
Published: August 2024

Genotypic and epigenetic considerations
Supplementary Appendix S1 from Tissue-Free Liquid Biopsies Combining Genomic and Methylation Signals for Minimal Residual Disease Detection in Patients with Early Colorectal Cancer from the UK TRACC Part B Study
Authors: Slater Susanna , Bryant Annette , Aresu Maria , Begum Ruwaida , Chen Hsiang-Chi , Peckitt Clare , Lazaro-Alcausi Retchel , Carter Paul , Anandappa Gayathri , Khakoo Shelize , Melcher Lucinda , Potter Vanessa , Marti Francisca M. , Huang Joesph , Branagan Graham , George Nicol , Abulafi Muti , Duff Sarah , Raja Ashraf , Gupta Ashish , West Nicholas , Bucheit Leslie , Rich Thereasa , Chau Ian , Cunningham David , Starling Naureen
Published: August 2024

TRACC B INVESTIGATORS
Supplementary Figure 4 from Targeting GPC2 on Intraocular and CNS Metastatic Retinoblastomas with Local and Systemic Delivery of CAR T Cells
Authors: Pascual-Pasto Guillem , McIntyre Brendan , Giudice Anna M. , Alikarami Fatemeh , Morrissey Amanda , Matlaga Stephanie , Hofmann Ted J. , Burgueño Victor , Harvey Kyra , Martinez Daniel , Shah Amish C. , Foster Jessica B. , Pogoriler Jennifer , Eagle Ralph C. , Carcaboso Angel M. , Shields Carol L. , Leahey Ann-Marie , Bosse Kristopher R.
Published: August 2024

Supplementary Figure 4. Mouse body weights and GPC2 expression in the brains of study endpoint mice included in the CNS efficacy study (see related main Fig. 5).
Supplementary Figure 2 from Targeting GPC2 on Intraocular and CNS Metastatic Retinoblastomas with Local and Systemic Delivery of CAR T Cells
Authors: Pascual-Pasto Guillem , McIntyre Brendan , Giudice Anna M. , Alikarami Fatemeh , Morrissey Amanda , Matlaga Stephanie , Hofmann Ted J. , Burgueño Victor , Harvey Kyra , Martinez Daniel , Shah Amish C. , Foster Jessica B. , Pogoriler Jennifer , Eagle Ralph C. , Carcaboso Angel M. , Shields Carol L. , Leahey Ann-Marie , Bosse Kristopher R.
Published: August 2024

Supplementary Figure 2. GPC2 CAR expression, activation, and polyfunctionality (see related main Fig. 3).
Supplementary Figure 1 from Targeting GPC2 on Intraocular and CNS Metastatic Retinoblastomas with Local and Systemic Delivery of CAR T Cells
Authors: Pascual-Pasto Guillem , McIntyre Brendan , Giudice Anna M. , Alikarami Fatemeh , Morrissey Amanda , Matlaga Stephanie , Hofmann Ted J. , Burgueño Victor , Harvey Kyra , Martinez Daniel , Shah Amish C. , Foster Jessica B. , Pogoriler Jennifer , Eagle Ralph C. , Carcaboso Angel M. , Shields Carol L. , Leahey Ann-Marie , Bosse Kristopher R.
Published: August 2024

Supplementary Figure 1. Expression of GPC2 and GD2 in retinoblastoma patient samples and cell lines (see related main Fig. 1).