bluuebunny/crossref_metadata_2025 huggingface dataset:
Nutritional Quality and Antioxidant Properties of Brown and Black Lentil Sprouts
Authors: Chatzimitakos Theodoros , Athanasiadis Vassilis , Makrygiannis Ioannis , Kotsou Konstantina , Palaiogiannis Dimitrios , Bozinou Eleni , Lalas Stavros I.
Published: June 2023

Lentils are known to be an integral part of a nutritionally balanced diet. Their sprouts are considered even more nutritional since they contain phytonutrients that confer health benefits. As such, incorporating them into a human diet can be advantageous. In this study, seeds from brown and black lentils were germinated aiming to study the changes in their nutritional value after they were grown for different amount of days to different lengths. Since the sprouts can be consumed at various stages of their growth, four growth stages were studied. For each stage, three batches were grown, and one sample of each batch was randomly picked and analyzed in triplicates. The sprouts were examined for their nutritional value. To this end, their content of proteins and carbohydrates was determined, as well as their content in carotenoids and vitamin C. Moreover, emphasis was placed on the phytochemical compounds contained in the sprouts. As results indicate, lentil sprouts not only exhibit high nutritional value but they are also rich in antioxidant compounds. More specifically, an increase of up to 18.8% in the protein content was recorded for 15 cm length sprouts (compared to lentil seeds), accompanied by a decrease in the carbohydrate content of up to 68.9%. Carotenoids and vitamin C content increased up to 224% and 389%, respectively. Additionally, a 34% increase in the polyphenol content was recorded. Moreover, a direct correlation between sprout length and nutritional value was observed, using principal component analysis (PCA) and multivariate correlation analysis (MCA).
Health System’s Role in Facilitating Health Service Access among Persons with Spinal Cord Injury across 22 Countries
Authors: Bychkovska Olena , Strøm Vegard , Tederko Piotr , Engkasan Julia Patrick , Juocevičius Alvydas , Battistella Linamara Rizzo , Arora Mohit , Egen Christoph , Gemperli Armin
Published: June 2023

(1) Background: Despite efforts to improve access to health services, between- and within-country access inequalities remain, especially for individuals with complex disabling conditions like spinal cord injury (SCI). Persons with SCI require regular multidisciplinary follow-up care yet experience more access barriers than the general population. This study examines health system characteristics associated with access among persons with SCI across 22 countries. (2) Methods: Study data are from the International Spinal Cord Injury Survey with 12,588 participants with SCI across 22 countries. Cluster analysis was used to identify service access clusters based on reported access restrictions. The association between service access and health system characteristics (health workforce, infrastructure density, health expenditure) was determined by means of classification and regression trees. (3) Results: Unmet needs were reported by 17% of participants: lowest (10%) in Japan, Spain, and Switzerland (cluster 1) and highest (62%) in Morocco (cluster 8). The country of residence was the most important factor in facilitating access. Those reporting access restrictions were more likely to live in Morocco, to be in the lowest income decile, with multiple comorbidities (Secondary Conditions Scale (SCI-SCS) score > 29) and low functioning status (Spinal Cord Independence Measure score < 53). Those less likely to report access restriction tended to reside in all other countries except Brazil, China, Malaysia, Morocco, Poland, South Africa, and South Korea and have fewer comorbidities (SCI-SCS < 23). (4) Conclusions: The country of residence was the most important factor in facilitating health service access. Following the country of residence, higher income and better health were the most important facilitators of service access. Health service availability and affordability were reported as the most frequent health access barriers.
Effect of Achillea fragrantissima Extract on Excision Wound Biofilms of MRSA and Pseudomonas aeruginosa in Diabetic Mice
Authors: Almuhanna Yasir , Alqasmi Mohammed Hussein , AlSudais Hamood , Alrouji Mohammed , Kuriri Fahd A. , Alissa Mohammed , Alsuwat Meshari A. , Asad Mohammed , Joseph Babu
Published: June 2023

Achillea fragrantissima, a desert plant commonly known as yarrow, is traditionally used as an antimicrobial agent in folklore medicine in Saudi Arabia. The current study was undertaken to determine its antibiofilm activity against methicillin-resistant Staphylococcus aureus (MRSA) and multi-drug-resistant Pseudomonas aeruginosa (MDR-P. aeruginosa) using in vitro and in vivo studies. A biofilm model induced through an excision wound in diabetic mice was used to evaluate its effect in vivo. The skin irritation and cytotoxic effects of the extract were determined using mice and HaCaT cell lines, respectively. The Achillea fragrantissima methanolic extract was analyzed with LC-MS to detect different phytoconstituents, which revealed the presence of 47 different phytoconstituents. The extract inhibited the growth of both tested pathogens in vitro. It also increased the healing of biofilm-formed excision wounds, demonstrating its antibiofilm, antimicrobial, and wound-healing action in vivo. The effect of the extract was concentration-dependent, and its activity was stronger against MRSA than MDR-P. aeruginosa. The extract formulation was devoid of a skin irritation effect in vivo and cytotoxic effect on HaCaT cell lines in vitro.
Evaluation of Pathogenicity and Causativity of Variants in the MPZ and SH3TC2 Genes in a Family Case of Hereditary Peripheral Neuropathy
Authors: Shchagina Olga , Orlova Mariya , Murtazina Aisylu , Filatova Alexandra , Skoblov Mikhail , Dadali Elena
Published: June 2023

The implementation of NGS methods into clinical practice allowed researchers effectively to establish the molecular cause of a disorder in cases of a genetically heterogeneous pathology. In cases of several potentially causative variants, we need additional analysis that can help in choosing a proper causative variant. In the current study, we described a family case of hereditary motor and sensory neuropathy (HMSN) type 1 (Charcot–Marie–Tooth disease). DNA analysis revealed two variants in the SH3TC2 gene (c.279G>A and c.1177+5G>A), as well as a previously described variant c.449−9C>T in the MPZ gene, in a heterozygous state. This family segregation study was incomplete because of the proband’s father's unavailability. To evaluate the variants’ pathogenicity, minigene splicing assay was carried out. This study showed no effect of the MPZ variant on splicing, but the c.1177+5G>A variant in the SH3TC2 gene leads to the retention of 122 nucleotides from intron 10 in the RNA sequence, causing a frameshift and an occurrence of a premature stop codon (NP_078853.2:p.Ala393GlyfsTer2).
Synthesis of Novel Antimicrobial CHX-CaCl2 Coatings on Maxillofacial Fixatures for Infection Prevention
Authors: Alostath Hawraa F. , Chatzopoulou Domniki , Holmes Simon , Gould David , Sukhorukov Gleb , Cattell Michael J.
Published: June 2023

Maxillofacial surgery placement of fixatures (Leonard Buttons, LB) at close proximity to surgical incisions provides a potential reservoir as a secondary local factor to advanced periodontal disease, with bacterial formation around failed fixatures implicating plaque. To address infection rates, we aimed to surface coat LB and Titanium (Ti) discs using a novel form of chlorhexidine (CHX), CHX-CaCl2 and 0.2% CHX digluconate mouthwash as a comparison. CHX-CaCl2 coated, double-coated and mouthwash coated LB and Ti discs were transferred to 1 mL artificial saliva (AS) at specified time points, and UV-Visible spectroscopy (254 nm) was used to measure CHX release. The zone of inhibition (ZOI) was measured using collected aliquots against bacterial strains. Specimens were characterized using Energy Dispersive X-ray Spectroscopy (EDS), X-ray Diffraction (XRD) and Scanning Electron Microscopy (SEM). SEM displayed copious dendritic crystals on LB/ Ti disc surfaces. Drug release from double-coated CHX-CaCl2 was 14 days (Ti discs) and 6 days (LB) above MIC, compared to the comparison group (20 min). The ZOI for the CHX-CaCl2 coated groups was significantly different within groups (p < 0.05). CHX-CaCl2 surface crystallization is a new drug technology for controlled and sustained CHX release; its antibacterial effectiveness makes this drug an ideal adjunct following clinical and surgical procedures to maintain oral hygiene and prevent surgical site infections.
New Insights into the Catalytic Activity of Second Generation Hoveyda–Grubbs Complexes Having Phenyl Substituents on the Backbone
Authors: D’Amato Assunta , Mariconda Annaluisa , Longo Pasquale
Published: June 2023

One of the most effective synthetic pathways to produce unsaturated compounds and polymers, meant for both industrial and pharmaceutical applications, is olefin metathesis. These useful reactions are commonly promoted by ruthenium-based precatalysts, namely the second-generation Grubbs’ catalyst (GII) and complexes bearing a styrenyl ether ligand, referred to as the second-generation Hoveyda–Grubbs’ catalyst (HGII). By altering the steric and electronic characteristics of substituents on the backbone and/or on the nitrogen atoms of the NHC ligand, it is possible to increase the reactivity and stability of second-generation ruthenium catalysts. The synthesis of an HG type II complex bearing two anti-phenyl backbone substituents (anti-HGIIPh-Mes) with mesityl N-substituents is reported. The catalytic performances of the new complex were investigated in standard ring-closing metathesis (RCM) and ring-opening metathesis polymerization (ROMP) and compared to those of the analogue complex syn-HGIIPh-Mes and to the classic HGII complex. A thorough analysis of the temperature dependence of the performances, along with a detailed comparison with the commercially available HGII, is conducted. The HGIIPh-Mes complexes are more thermally stable than their parent HGII, as shown by the fact that their activity in the ROMP of 5-ethylidene-2-norbornene does not alter when the polymerization is carried out at room temperature after the complexes have been held at 180 °C for two hours, making them particularly interesting for materials applications.
Collembola of the Cavalum and Landeiro Caves (Madeira, Portugal)
Authors: Baquero Enrique , Arbea Javier I. , Nunes Élvio , Aguin-Pombo Dora , Mateos Eduardo , Jordana Rafael
Published: June 2023

The cave fauna of the Macaronesian archipelagos is rich in endemic species. Unlike the Azores and the Canary Islands, the cave fauna of the Madeira archipelago is little known. The only two cave complexes studied, Machico and São Vicente, lack protection measures. São Vicente is seriously threatened because it is being exploited for touristic purposes, while the Machico complex, the only one in its natural state, is open to the public but without any control. The importance of the conservation of this cave fauna is indisputable. So far, of the 13 cavernicolous species recorded, two of them—genus Centromerus—are critically endangered. Apart from occasional sampling, no monitoring study has ever been carried out. The aim of this work has been to make a species checklist of the cave fauna of the Machico complex, the least studied so far. For this purpose, during 2001–2002, a monitoring study was carried out using traps and manual collections in the lava tubes of Landeiros and Cavalum (I, II, III). Fourteen species of springtails were recorded. Of these, four are new species, Neelus serratus Jordana & Baquero sp. nov., Coecobrya decemsetosa Jordana & Baquero sp. nov., Coecobrya octoseta Jordana & Baquero sp. nov., and Sinella duodecimoculata Jordana & Baquero sp. nov., and one is a new record for the archipelago, Lepidocyrtus curvicollis Bourlet, 1839.
Open-Source Designs for Distributed Manufacturing of Low-Cost Customized Walkers
Authors: So Anita , Reeves Jacob M. , Pearce Joshua M.
Published: June 2023

To improve accessibility, this article describes a static, four-legged walker that can be constructed from materials and fasteners commonly available from hardware stores coupled by open-source 3D-printed joints. The designs are described in detail, shared under an open-source license, and fabricated with a low-cost open-source desktop 3D printer and hand tools. The resulting device is loaded to failure to determine the maximum load that the design can safely support in both vertical and horizontal failure modes. The experimental results showed that the average vertical failure load capacity was 3680 ± 694.3 N, equivalent to 375.3 ± 70.8 kg of applied weight with the fractured location at the wood dowel handlebars. The average horizontal load capacity was 315.6 ± 49.4 N, equivalent to 32.2 ± 5.1 kg. The maximum weight capacity of a user of 187.1 ± 29.3 kg was obtained, which indicates that the open-source walker design can withstand the weight requirements of all genders with a 95% confidence interval that includes a safety factor of 1.8 when considering the lowest deviation weight capacity. The design has a cost at the bottom of the range of commercial walkers and reduces the mass compared to a commercial walker by 0.5 kg (19% reduction). It can be concluded that this open-source walker design can aid accessibility in low-resource settings.
Severe Asthma in the Era of Biologics: Continuous Challenges
Authors: Schoini Pinelopi , Bakakos Petros , Loukides Stelios
Published: June 2023

Asthma is a heterogenous disease characterized by different phenotypes and endotypes [...]
Consideration of Psychosocial Factors in Acute Low Back Pain by Physical Therapists
Authors: Otero-Ketterer Emilia , Peñacoba-Puente Cecilia , Ortega-Santiago Ricardo , Galán-del-Río Fernando , Valera-Calero Juan Antonio
Published: June 2023

Clinical guidelines consistently recommend screening psychosocial (PS) factors in patients with low back pain (LBP), regardless of its mechanical nature, as recognized contributors to pain chronicity. However, the ability of physiotherapists (PTs) in identifying these factors remains controversial. This study aimed to assess the current identification of psychosocial risk factors by physical therapists (PTs) and which characteristics of PTs are associated with the identification of the main risk for chronicity (physical or psychosocial). A cross-sectional descriptive study surveying Spanish PTs in public and private health services was conducted, including questions on PT characteristics and three low back pain (LBP) patient vignettes with different biopsychosocial (BPS) clinical presentations. From 484 respondents, the majority of PTs agreed regarding the main risk for chronicity for each vignette (PS 95.7% for vignette A, PS and physical 83.5% for vignette B and PS 66% for vignette C). Female PTs were more likely to rate psychosocial compared with males (p < 0.05). PTs with higher levels of social and emotional intelligence (both, p < 0.05) were more likely to identify the main risk for chronicity. However, only gender and social information processing for vignette A (p = 0.024) and emotional clarity for vignette B (p = 0.006) were able to predict the identification of psychosocial and physical risk, respectively. The main risk for chronicity was correctly identified by a large majority of PTs through patient vignettes. Gender, social and emotional intelligence played a relevant role in the recognition of psychosocial risk and biopsychosocial factors.
Efficient GEMM Implementation for Vision-Based Object Detection in Autonomous Driving Applications
Authors: Guerrouj Fatima Zahra , Rodríguez Flórez Sergio , Abouzahir Mohamed , El Ouardi Abdelhafid , Ramzi Mustapha
Published: June 2023

Convolutional Neural Networks (CNNs) have been incredibly effective for object detection tasks. YOLOv4 is a state-of-the-art object detection algorithm designed for embedded systems. It is based on YOLOv3 and has improved accuracy, speed, and robustness. However, deploying CNNs on embedded systems such as Field Programmable Gate Arrays (FPGAs) is difficult due to their limited resources. To address this issue, FPGA-based CNN architectures have been developed to improve the resource utilization of CNNs, resulting in improved accuracy and speed. This paper examines the use of General Matrix Multiplication Operations (GEMM) to accelerate the execution of YOLOv4 on embedded systems. It reviews the most recent GEMM implementations and evaluates their accuracy and robustness. It also discusses the challenges of deploying YOLOv4 on autonomous vehicle datasets. Finally, the paper presents a case study demonstrating the successful implementation of YOLOv4 on an Intel Arria 10 embedded system using GEMM.
An Updated Systematic Review and Meta-Analysis of the Association between the De Ritis Ratio and Disease Severity and Mortality in Patients with COVID-19
Authors: Mangoni Arduino A. , Zinellu Angelo
Published: June 2023

Patients with Coronavirus disease 2019 (COVID-19) often have elevations in markers of liver injury, particularly serum aspartate transaminase (AST) and alanine transaminase (ALT). Such alterations may affect the AST/ALT ratio (De Ritis ratio) and, potentially, clinical outcomes. We conducted an updated systematic review and meta-analysis of the association between the De Ritis ratio and COVID-19 severity and mortality in hospitalized patients. PubMed, Web of Science, and Scopus were searched between 1 December 2019 and 15 February 2023. The Joanna Briggs Institute Critical Appraisal Checklist and the Grading of Recommendations, Assessment, Development, and Evaluation were used to assess the risk of bias and the certainty of the evidence, respectively. Twenty-four studies were identified. The De Ritis ratio on admission was significantly higher in patients with severe disease and non-survivors vs. patients with non-severe disease and survivors (15 studies, weighted mean difference = 0.36, 95% CI 0.24 to 0.49, p < 0.001). The De Ritis ratio was also associated with severe disease and/or mortality using odds ratios (1.83, 95% CI 1.40 to 2.39, p ˂ 0.001; nine studies). Similar results were observed using hazard ratios (2.36, 95% CI 1.17 to 4.79, p = 0.017; five studies). In six studies, the pooled area under the receiver operating characteristic curve was 0.677 (95% CI 0.612 to 0.743). In our systematic review and meta-analysis, higher De Ritis ratios were significantly associated with severe disease and mortality in COVID-19 patients. Therefore, the De Ritis ratio can be useful for early risk stratification and management in this patient group (PROSPERO registration number: CRD42023406916).
Effectiveness of One-Year Pemafibrate Therapy on Non-Alcoholic Fatty Liver Disease Refractory to Long-Term Sodium Glucose Cotransporter-2 Inhibitor Therapy: A Pilot Study
Authors: Shinozaki Satoshi , Tahara Toshiyuki , Miura Kouichi , Lefor Alan Kawarai , Yamamoto Hironori
Published: June 2023

Background: Both pemafibrate and sodium glucose cotransporter-2 (SGLT2) inhibitor can decrease serum transaminase levels in patients with non-alcoholic fatty liver disease (NAFLD) complicated with dyslipidemia and type 2 diabetes mellitus (T2DM), respectively. However, the effectiveness of combined therapy has been rarely reported. Methods: This is a two-center retrospective observational study. NAFLD patients complicated with T2DM treated with pemafibrate for >1 year were included, in whom prior treatment with SGLT2 inhibitor > 1 year failed to normalize serum alanine aminotransferase (ALT) levels. Hepatic inflammation, function, and fibrosis were assessed by ALT, albumin-bilirubin (ALBI) score, and Mac-2 binding protein glycosylation isomer (M2BPGi) levels, respectively. Results: Seven patients were included. The median duration of prior treatment with SGLT2 inhibitors was 2.3 years. During the one year before starting pemafibrate therapy, the therapy did not significantly change hepatic enzymes. All patients received pemafibrate 0.1 mg twice daily without dose escalations. During one year of pemafibrate therapy, triglyceride, aspartate aminotransferase, ALT, γ-glutamyl transpeptidase, ALBI score, and M2BPGi levels significantly improved (p < 0.05), although weight or hemoglobin A1c did not significantly change. Conclusions: One year of pemafibrate therapy improves markers of hepatic inflammation, function, and fibrosis in NAFLD patients in whom long-term SGLT2 inhibitor therapy failed to normalize serum ALT.
The Importance of the Nephrologist in the Treatment of the Diuretic-Resistant Heart Failure
Authors: Pethő Ákos Géza , Tapolyai Mihály , Browne Maria , Fülöp Tibor , Orosz Petronella , Szabó Réka P.
Published: June 2023

Heart failure is not only a global problem but also significantly limits the life prospects of these patients. The epidemiology and presentation of heart failure are intensively researched topics in cardiology. The risk factors leading to heart failure are well known; however, the real challenge is to provide effective treatments. A vicious cycle develops in heart failure of all etiologies, sooner or later compromising both cardiac and kidney functions simultaneously. This can explain the repeated hospital admissions due to decompensation and the significantly reduced quality of life. Moreover, diuretic-refractory heart failure represents a distinct challenge due to repeated hospital admissions and increased mortality. In our narrative review, we wanted to draw attention to nephrology treatment options for severe diuretic-resistant heart failure. The incremental value of peritoneal dialysis in severe heart failure and the feasibility of percutaneous peritoneal dialysis catheter insertion have been well known for many years. In contrast, the science and narrative of acute peritoneal dialysis in diuretic-resistant heart failure remains underrepresented. We believe that nephrologists are uniquely positioned to help these patients by providing acute peritoneal dialysis to reduce hospitalization dependency and increase their quality of life.
Numerical Calculation Method of Multi-Lip Seal Wear under Mixed Thermal Elastohydrodynamic Lubrication
Authors: Cheng Donghong , Gu Lichen , Sun Yu , Shi Yuan
Published: June 2023

The multi-lip combined seal has the advantages of multi-lip coupling, large supporting force, and can withstand high-pressure shocks. It is an irreplaceable structure for single-lip seals. However, most of the seal wear analysis focuses on the simulation method of the single-lip seal under the influence of macro factors, and very little involves the wear characteristics of multi-lip seals. In this paper, a micro numerical method is established, which combines the elastohydrodynamic lubrication theory with the modified Archard equation. The performance of a multi-lip combined seal under different working conditions is analyzed through simulation, including macro and micro factors. It is found that some of the characteristics of single-lip seals are also reflected in multi-lip seals, and there is a critical speed that makes the sealing behavior of each seal lip different.
A Markov Decision Process with Awareness and Present Bias in Decision-Making
Authors: Bizzarri Federico , Mocenni Chiara , Tiezzi Silvia
Published: June 2023

We propose a Markov Decision Process Model that blends ideas from Psychological research and Economics to study decision-making in individuals with self-control problems. We have borrowed a dual-process of decision-making with self-awareness from Psychological research, and we introduce present bias in inter-temporal preferences, a phenomenon widely explored in Economics. We allow for both an exogenous and endogenous, state-dependent, present bias in inter-temporal decision-making and explore, by means of numerical simulations, the consequences on well-being emerging from the solution of the model. We show that, over time, self-awareness may mitigate present bias and suboptimal choice behaviour.
Ranking the Importance of Variables in a Nonparametric Frontier Analysis Using Unsupervised Machine Learning Techniques
Authors: Moragues Raul , Aparicio Juan , Esteve Miriam
Published: June 2023

In this paper, we propose and compare new methodologies for ranking the importance of variables in productive processes via an adaptation of OneClass Support Vector Machines. In particular, we adapt two methodologies inspired by the machine learning literature: one involving the random shuffling of values of a variable and another one using the objective value of the dual formulation of the model. Additionally, we motivate the use of these type of algorithms in the production context and compare their performance via a computational experiment. We observe that the methodology based on shuffling the values of a variable outperforms the methodology based on the dual formulation. We observe that the shuffling-based methodology correctly ranks the variables in 94% of the scenarios with one relevant input and one irrelevant input. Moreover, it correctly ranks each variable in at least 65% of replications of a scenario with three relevant inputs and one irrelevant input.
A Novel Adaptive Manta-Ray Foraging Optimization for Stochastic ORPD Considering Uncertainties of Wind Power and Load Demand
Authors: Almutairi Sulaiman Z. , Mohamed Emad A. , El-Sousy Fayez F. M.
Published: June 2023

The optimal control of reactive powers in electrical systems can improve a system’s performance and security; this can be provided by the optimal reactive power dispatch (ORPD). Under the high penetration of renewable energy resources (RERs) such as wind turbines (WTs), the ORPD problem solution has become a challenging and complex task due to the fluctuations and uncertainties of generated power from WTs. In this regard, this paper solved the conventional ORPD and the stochastic ORPD (SORPD) at uncertainties of the generated power from WTs and the load demand. An Adaptive Manta-Ray Foraging Optimization (AMRFO) was presented based on three modifications, including the fitness distance balance selection (FDB), Quasi Oppositional based learning (QOBL), and an adaptive Levy Flight (ALF). The ORPD and SORPD were solved to reduce the power loss (PLoss) and the total expected PLoss (TEPL), the voltage deviations (VD) and the total expected VD (TEVD). The normal and Weibull probability density functions (PDFs), along with the scenario reduction method and the Monte Carlo simulation (MCS), were utilized for uncertainty representations. The performance and validity of the suggested AMRFO were compared to other optimizers, including SCSO, WOA, DO, AHA, and the conventional MRFO on the IEEE 30-bus system and standard benchmark functions. These simulation results confirm the supremacy of the suggested AMRFO for the ORPD and SORPD solution compared to the other reported techniques.
Automated Model Selection of the Two-Layer Mixtures of Gaussian Process Functional Regressions for Curve Clustering and Prediction
Authors: Gong Chengxin , Ma Jinwen
Published: June 2023

As a reasonable statistical learning model for curve clustering analysis, the two-layer mixtures of Gaussian process functional regressions (TMGPFR) model has been developed to fit the data of sample curves from a number of independent information sources or stochastic processes. Since the sample curves from a certain stochastic process naturally form a curve cluster, the model selection of TMGPFRs, i.e., the selection of the number of mixtures of Gaussian process functional regressions (MGPFRs) in the upper layer, corresponds to the discovery of the cluster number and structure of the curve data. In fact, this is rather challenging because the conventional model selection criteria, such as BIC and cross-validation, cannot lead to a stable result in practice even with a heavy burden of repetitive computation. In this paper, we improve the original TMGPFR model and propose a Bayesian Ying-Yang (BYY) annealing learning algorithm for the parameter learning of the improved model with automated model selection. The experimental results of both synthetic and realistic datasets demonstrate that our proposed algorithm can make correct model selection automatically during parameter learning of the model.
Physical Conditions Prevailing in the Nasal and Maxillary Sinus Cavities Based on Numerical Simulation
Authors: Morawska-Kochman Monika , Malecha Ziemowit Miłosz , Zub Krzysztof , Kielar Jakub , Dudek Krzysztof , Nelke Kamil , Zatonski Tomasz
Published: June 2023

Background and Objectives: This paper presents a unique study that links the physical conditions in the nasal passage with conditions that favour the development of bacterial strains and the colonization of the mucous membranes of the nose and paranasal sinuses. The physical parameters considered were air flow, pressure, humidity, and temperature. Materials and Methods: Numerical models of the human nose and maxillary sinus were retrospectively reconstructed from CT images of generally healthy young subjects. The state-of-the-art numerical methods and tools were then used to determine the temperature, humidity, airflow velocity, and pressure at specific anatomical locations. Results: The results were compared with optimal conditions for bacterial growth in the nose and sinuses. Conclusions: Temperature, humidity, air velocity, and pressure were shown to play critical roles in the selection and distribution of microorganisms. Furthermore, certain combinations of physical parameters can favour mucosal colonisation by various strains of bacteria.
An Electrochemistry and Computational Study at an Electrified Liquid–Liquid Interface for Studying Beta-Amyloid Aggregation
Authors: Silwane Bongiwe , Wilson Mark , Kataky Ritu
Published: June 2023

Amphiphilic peptides, such as Aß amyloids, can adsorb at an interface between two immiscible electrolyte solutions (ITIES). Based on previous work (vide infra), a hydrophilic/hydrophobic interface is used as a simple biomimetic system for studying drug interactions. The ITIES provides a 2D interface to study ion-transfer processes associated with aggregation, as a function of Galvani potential difference. Here, the aggregation/complexation behaviour of Aβ(1-42) is studied in the presence of Cu (II) ions, together with the effect of a multifunctional peptidomimetic inhibitor (P6). Cyclic and differential pulse voltammetry proved to be particularly sensitive to the detection of the complexation and aggregation of Aβ(1-42), enabling estimations of changes in lipophilicity upon binding to Cu (II) and P6. At a 1:1 ratio of Cu (II):Aβ(1-42), fresh samples showed a single DPV (Differential Pulse Voltammetry) peak half wave transfer potential (E1/2) at 0.40 V. Upon increasing the ratio of Cu (II) two-fold, fluctuations were observed in the DPVs, indicating aggregation. The approximate stoichiometry and binding properties of Aβ(1-42) during complexation with Cu (II) were determined by performing a differential pulse voltammetry (DPV) standard addition method, which showed two binding regimes. A pKa of 8.1 was estimated, with a Cu:Aβ1-42 ratio~1:1.7. Studies using molecular dynamics simulations of peptides at the ITIES show that Aβ(1-42) strands interact through the formation of β-sheet stabilised structures. In the absence of copper, binding/unbinding is dynamic, and interactions are relatively weak, leading to the observation of parallel and anti-parallel arrangements of β-sheet stabilised aggregates. In the presence of copper ions, strong binding occurs between a copper ion and histidine residues on two peptides. This provides a convenient geometry for inducing favourable interactions between folded β-sheet structures. Circular Dichroism spectroscopy (CD spectroscopy) was used to support the aggregation behaviour of the Aβ(1-42) peptides following the addition of Cu (II) and P6 to the aqueous phase.
Design of 2 μm Low-Loss Hollow-Core Anti-Resonant Fibers
Authors: Sun Tianran , Su Xinyang , Meng Fanchao , Wang Zaining , Song Jiale , Zhang Chenglong , Xu Tianjia , Zhang Yunhong , Zhang Huaiwei , Cui Mengdi , Zheng Yi
Published: June 2023

We systematically studied several of the most traditional hollow-core anti-resonant fiber (HC-ARF) structures, with the aim of achieving low confinement loss, single-mode performance, and high insensitivity to bending in the 2 µm band. Moreover, the propagation loss of fundamental mode (FM), higher-order mode (HOMs), and the higher-order mode extinction ratio (HOMER) under different geometric parameters were studied. Analysis showed that the confinement loss of the six-tube nodeless hollow-core anti-resonant fiber at 2 µm was 0.042 dB/km, and its higher-order mode extinction ratio was higher than 9000. At the same time, a confinement loss of 0.040 dB/km at 2 µm was achieved in the five-tube nodeless hollow-core anti-resonant fiber, and its higher-order mode extinction ratio was higher than 2700.
Synchrotron Microanalytical Characterization and K/Ar Dating of the GL-O-1 Glauconite Reference Material at the Single Pellet Scale and Reassessment of the Age of Visually Mature Pellets
Authors: Hemming Sidney R. , Liu Tanzhuo , Northrup Paul , Nicholas Sarah , Rasbury E. Troy , Chen Heng , Warden Alice , Chen Amanda , Li Ruipeng , Tappero Ryan , Cox Stephen E. , Everard Jenna , Wang Silu , Deluca Michael , Bostick Benjamin , Halliday Alexander N.
Published: June 2023

The K/Ar chronology of glauconite pellets is a long-used method for directly dating marine sedimentary deposits. Many papers have explored the processes that form glauconite and the factors that lead to greater reliability in the ages. Although K/Ar ages of glauconite are generally in agreement with other measures of stratigraphic age, there are examples of occurrences with ages too old and examples with ages too young. This paper seeks to build on the accumulated knowledge of glauconite, using synchrotron radiation to non-destructively characterize individual pellets and then consecutively measure the argon and potassium to obtain a K/Ar age. This strategy provides the advantage of measurements on a single aliquot while avoiding recoil loss of 40Ar in the nuclear reactor during irradiation for 40Ar/39Ar dating. We have used the glauconite reference material GL-O-1 to showcase several non-destructive methods for evaluating the maturity of individual pellets. In our argon measurements, we have found that the radiogenic argon concentration of large bulk samples underestimates the values for individual visually mature pellets, and we determined a K/Ar age of 101.0 ± 0.3 Ma (1σ SEM), M.S.W.D. 0.54 from 15 of 16 visually mature individual pellets. This age is 6% older than the reference value of 95.03 ± 1.11 Ma (1σ), and it is in good agreement with constraints from the U-Pb dating of volcanic minerals near the Albian–Cenomanian boundary.
Correction: Grancharov et al. Flexible Polymer–Organic Solar Cells Based on P3HT:PCBM Bulk Heterojunction Active Layer Constructed under Environmental Conditions. Molecules 2021, 26, 6890
Authors: Grancharov Georgy , Atanasova Mariya-Desislava , Kalinova Radostina , Gergova Rositsa , Popkirov Georgi , Dikov Christosko , Sendova-Vassileva Marushka
Published: June 2023

The authors would like to correct a mistake in Figure 3 as published in the original publication [...]
Multi-Detection Size Exclusion Chromatography as an Advanced Tool for Monitoring Enzyme–Antibody Conjugation Reaction and Quality Control of a Final Product
Authors: Štimac Adela , Kurtović Tihana , Halassy Beata
Published: June 2023

The multi-detection size exclusion chromatography (SEC) has been recognized as an advanced analytical technique for the characterization of macromolecules and process control, as well as the manufacturing and formulation of biotechnology products. It reveals reproducible molecular characterization data, such as molecular weight and its distribution, and the size, shape, and composition of the sample peaks. The aim of this work was to investigate the potential and suitability of the multi-detection SEC as a tool for surveillance over the molecular processes during the conjugation reaction between the antibody (IgG) and horseradish peroxidase (HRP), and demonstrate the plausibility of its application in the quality control of the final product, the IgG-HRP conjugate. Guinea pig anti-Vero IgG-HRP conjugate was prepared using a modified periodate oxidation method, based on periodate oxidation of the carbohydrate side chains of HRP, followed by the formation of Schiff bases between the activated HRP and amino groups of the IgG. The quantitative molecular characterization data of the starting samples, intermediates, and final product were obtained by multi-detection SEC. Titration of the prepared conjugate was performed by the ELISA and its optimal working dilution was determined. This methodology proved to be a promising and powerful technology for the IgG-HRP conjugate process control and development, as well as for the quality control of the final product, as verified by the analysis of several commercially available reagents.
Preparation of Gelatin-Quaternary Ammonium Salt Coating on Titanium Surface for Antibacterial/Osteogenic Properties
Authors: Song Hongyang , Xing Lei , Wei Jinjian , Wang Xue , Yang Yaozhen , Zhe Pengbo , Luan Mingming , Xu Jing
Published: June 2023

Titanium (Ti) and its alloys are widely used in medical treatment, engineering, and other fields because of their excellent properties including biological activity, an elastic modulus similar to that of human bones, and corrosion resistance. However, there are still many defects in the surface properties of Ti in practical applications. For example, the biocompatibility of Ti with bone tissue can be greatly reduced in implants due to a lack of osseointegration as well as antibacterial properties, which may lead to osseointegration failure. To address these problems and to take advantage of the amphoteric polyelectrolyte properties of gelatin, a thin layer of gelatin was prepared by electrostatic self-assembly technology. Diepoxide quaternary ammonium salt (DEQAS) and maleopimaric acid quaternary ammonium salt (MPA−N+) were then synthesized and grafted onto the thin layer. The cell adhesion and migration experiments demonstrated that the coating has excellent biocompatibility, and those grafted with MPA−N+ promoted cell migration. The bacteriostatic experiment showed that the mixed grafting with two ammonium salts had excellent bacteriostatic performance against Escherichia coli and Staphylococcus aureus, with bacteriostasis rates of 98.1 ± 1.0% and 99.2 ± 0.5%, respectively.
Comparison of Consumption of Pulses in Two Seasons of the Year in Chile
Authors: Barrientos-De la Rosa Claudia , Duran-Aguero Samuel , Mardones María José , Morejón Yadira , García-Milla Paula , Albornoz Pablo , Torres Ximena , Landaeta-Díaz Leslie
Published: June 2023

Background: In Chile, the consumption of legumes at least two times per week is promoted. However, there is a low consumption of legumes. Therefore, our objective is to describe legume consumption in two different seasonal periods. Methods: Serial cross-sectional study: surveys were distributed during summer and winter using different digital platforms. Frequency of consumption, purchase access, and preparation type were investigated. Results: In total, 3280 adults were surveyed in summer and 3339 in winter. The mean age was 33 years. Totals of 97.7% and 97.5% of the population reported consuming legumes in both periods; consumption increased to 3 times per week during winter. In both periods, the main reason for their preference is that they are delicious and nutritious, followed by their use as a meat substitute; the main barriers to their consumption in both periods are that they are expensive (29% in summer and 27.8% in winter) and difficult to prepare. Conclusion: A good consumption of legumes was observed, but with a higher frequency of consumption during winter, with an intake of ≥1 per day; additionally, differences were found in purchases according to season, although no differences were found in the method of preparation.
Comparison of Vitamin D and Resveratrol Performances in COVID-19
Authors: Russo Cristina , Valle Maria Stella , Malaguarnera Luisa , Romano Ivana Roberta , Malaguarnera Lucia
Published: June 2023

Over the last few years, we have experienced the infection generated by severe respiratory syndrome coronavirus 2 (SARS-CoV-2) often resulting in an exaggerated immune reaction and systemic inflammation. The preferred treatments against SARS-CoV-2 were those that mitigated immunological/inflammatory dysfunction. A variety of observational epidemiological studies have reported that vitamin D deficiency is often a crucial factor in many inflammatory diseases and autoimmune diseases, as well as the susceptibility to contract infectious diseases, including acute respiratory infections. Similarly, resveratrol regulates immunity, modifying the gene expression and the release of proinflammatory cytokines in the immune cells. Therefore, it plays an immunomodulatory role that can be beneficial in the prevention and development of non-communicable diseases associated with inflammation. Since both vitamin D and resveratrol also act as immunomodulators in inflammatory pathologies, many studies have paid particular attention to an integrated treatment of either vitamin D or resveratrol in the immune reaction against SARS-CoV-2 infections. This article offers a critical evaluation of published clinical trials that have examined the use of vitamin D or resveratrol as adjuncts in COVID-19 management. Furthermore, we aimed to compare the anti-inflammatory and antioxidant properties linked to the modulation of the immune system, along with antiviral properties of both vitamin D and resveratrol.
Antibacterial and Biofilm Production Inhibition Activity of Thymus vulgaris L. Essential Oil against Salmonella spp. Isolates from Reptiles
Authors: Galgano Michela , Mrenoshki Daniela , Pellegrini Francesco , Capozzi Loredana , Cordisco Marco , Del Sambro Laura , Trotta Adriana , Camero Michele , Tempesta Maria , Buonavoglia Domenico , Laricchiuta Piero , Catella Cristiana , Pratelli Annamaria , Buonavoglia Alessio , Corrente Marialaura
Published: June 2023

Salmonellosis is an infectious disease affecting both animals and humans. Antimicrobial resistant (AMR) and biofilm-producing Salmonella spp., frequently detected in reptiles (who can then act as asymptomatic carriers for warm-blooded animals), have developed resistance to biocides; this represents a warning for the emergence of biocide/antimicrobial cross-resistance. The aim of this study was to evaluate the efficacy of Thymus vulgaris L. essential oil (TEO) in inhibiting bacterial growth and biofilm production of Salmonella spp., which had been isolated from wild reptiles housed in a Zoo in Italy. The resistance profile against different classes of antibiotics showed that all the isolates were susceptible to the tested antibiotics, despite the presence of several AMR genes. All the isolates were also tested with aqueous solutions of TEO at different dilutions (5% to 0.039%). Interestingly, TEO proved effective both in inhibiting bacterial growth at low dilutions, with MIC and MBC values ranging between 0.078% and 0.312%, and in inhibiting biofilm production, with values ranging from 0.039% to 0.156%. TEO demonstrated effective bioactivity against the biofilm producer Salmonella spp., proving to be a valid disinfectant for the prevention of salmonellosis from reptiles, a possible source of infection for humans exposed to the reptiles’ environment.
Negative Outcomes Associated with Medication in Neonates on Parenteral Nutrition Therapy
Authors: Vega Díaz Evelin Nataly , Miranda Barros Aida Adriana , Castelo Reyna Monica Alexandra , Tenelanda López Dennys , Tubon Irvin
Published: June 2023

Objective: In Ecuador, studies on clinical daily practice problems focused on parenteral nutrition in neonates are scarce. Therefore, this research aimed to identify negative results associated with medications (NRAM) in neonates with parenteral nutrition (PN) in a third-level hospital in Ecuador. Material and methods: An observational, prospective, descriptive study was designed in the neonatology area of a tertiary-level public hospital, where, for over four months, the medical records, PN prescriptions, and pharmacy-managed databases of 78 patients were analyzed. Drug-related problems (DRPs) as possible causes of NRAM were classified through administrative, physicochemical, and clinical validation. Results: DRPs classified as follows were found: 78.81% by physicochemical, 17.62% by clinical, and 3.57% by administrative validation. The NRAM were 72% quantitatively uncertain, 16% needed, and 11% quantitatively ineffective. Conclusion: The NRAM associated with DRPs were statistically related to prematurity condition, APGAR score, PN time, and the number of medications administered, which suggests the need to create a nutritional therapy committee at the health facility.
Wettability of Amino Acid-Functionalized PSMA Electrospun Fibers for the Modulated Release of Active Agents and Its Effect on Their Bioactivity
Authors: Santander Sebastián , Padilla-Manzano Nicolás , Díaz Bastián , Bacchiega Renato , Jara Elizabeth , Álvarez Luis Felipe , Pinto Cristóbal , Forero Juan C. , Santana Paula , Hamm Eugenio , Urzúa Marcela , Tamayo Laura
Published: June 2023

The ideal treatment for chronic wounds is based on the use of bioactive dressings capable of releasing active agents. However, the control of the rate at which these active agents are released is still a challenge. Bioactive polymeric fiber mats of poly(styrene-co-maleic anhydride) [PSMA] functionalized with amino acids of different hydropathic indices and L-glutamine, L-phenylalanine and L-tyrosine levels allowed obtaining derivatives of the copolymers named PSMA@Gln, PSMA@Phe and PSMA@Tyr, respectively, with the aim of modulating the wettability of the mats. The bioactive characteristics of mats were obtained by the incorporation of the active agents Calendula officinalis (Cal) and silver nanoparticles (AgNPs). A higher wettability for PSMA@Gln was observed, which is in accordance with the hydropathic index value of the amino acid. However, the release of AgNPs was higher for PSMA and more controlled for functionalized PSMA (PSMAf), while the release curves of Cal did not show behavior related to the wettability of the mats due to the apolar character of the active agent. Finally, the differences in the wettability of the mats also affected their bioactivity, which was evaluated in bacterial cultures of Staphylococcus aureus ATCC 25923 and methicillin-resistant Staphylococcus aureus ATCC 33592, an NIH/3T3 fibroblast cell line and red blood cells.
Magnetic Nanoparticles for Therapy and Diagnosis in Nanomedicine
Authors: Mamani Javier Bustamante , Borges João Paulo , Rossi Alexandre Malta , Gamarra Lionel Fernel
Published: June 2023

Magnetic nanoparticles (MNPs) have been widely used for their potential applications, mainly for the diagnosis and/or therapy (theranostic) of several diseases in the field of nanomedicine, as passive contrast agents, through the opsonization process, or active contrast agents, after their functionalization and the subsequent capture of the signal using various techniques such as magnetic resonance imaging (MRI), optical imaging, nuclear imaging, and ultrasound [...]