bluuebunny/crossref_metadata_2025 huggingface dataset:
Nature-Inspired Optimization Algorithms for Text Document Clustering—A Comprehensive Analysis
Authors: Abualigah Laith , Gandomi Amir H. , Elaziz Mohamed Abd , Hussien Abdelazim G. , Khasawneh Ahmad M. , Alshinwan Mohammad , Houssein Essam H.
Published: December 2020

Text clustering is one of the efficient unsupervised learning techniques used to partition a huge number of text documents into a subset of clusters. In which, each cluster contains similar documents and the clusters contain dissimilar text documents. Nature-inspired optimization algorithms have been successfully used to solve various optimization problems, including text document clustering problems. In this paper, a comprehensive review is presented to show the most related nature-inspired algorithms that have been used in solving the text clustering problem. Moreover, comprehensive experiments are conducted and analyzed to show the performance of the common well-know nature-inspired optimization algorithms in solving the text document clustering problems including Harmony Search (HS) Algorithm, Genetic Algorithm (GA), Particle Swarm Optimization (PSO) Algorithm, Ant Colony Optimization (ACO), Krill Herd Algorithm (KHA), Cuckoo Search (CS) Algorithm, Gray Wolf Optimizer (GWO), and Bat-inspired Algorithm (BA). Seven text benchmark datasets are used to validate the performance of the tested algorithms. The results showed that the performance of the well-known nurture-inspired optimization algorithms almost the same with slight differences. For improvement purposes, new modified versions of the tested algorithms can be proposed and tested to tackle the text clustering problems.
Experimental and CFD Investigation into Using Inverted U-Tube for Gas Entrainment
Authors: Yousef Khaled , Hegazy Ahmed , Engeda Abraham
Published: December 2020

An experimental and numerical study is presented in the current work for gas entrainment using an inverted vertical U-tube. Water flows vertically up in an inverted U-tube which creates a low-pressure region in the tube upper portion. This low-pressure region can be used to extract gases by connecting it to a branch pipe. The extracted gases considered in this work are a mixture of air and water vapor. The water vapor from the side branch pipe is mixed with the flowing water under the siphon effect. This results in a progressive water vapor condensation as the mixture proceeds towards the exit due to an increase in vapor partial pressure. The air is drawn by inertia to be released out at the tube lower exit of the inverted U-pipe. The current study deals with these complicated flow behaviors due to the mixing undergoing condensation. A test rig is designed for experimentally studying the behavior of water flow in an inverted U-tube where the air is mixed with the flowing water at the top region of this tube. The CFD computations are accomplished for a side gas mixture with volume fractions up to 0.7 with water vapor mass fractions in this mixture to be 0.1–0.5. The tested water mass flow rates in the main tube are 2, 4, 6, 8 kg/s to account for all possible flow mass ratios. The CFD computations are validated with water and air two phase flow with the measurements of both the experiments of the current research and the literature. The present results reveal that slightly raising the water mass flow rate at a constant side mixture mass ratio produces a reduced generated pressure in the upper tube part. This is attributed to extra water vapor condensation taking place rapidly by increasing the water flow rate in the tube upper part. Furthermore, the turbulence quantities begin to break down at a side mixture volume fraction of 0.55 with water and air mass flow rates of 2 kg/s and 0.002 kg/s, respectively. On the other side, raising the air mass flow rate at the higher values of water vapor and water mass flow rates breaks the generated vacuum pressure and turbulence due to entrainment. Moreover, this proposed framework can produce a lower static pressure, reaching 55.1 kPa, which makes it attractive for gas extraction. This new technique presents innovative usage with less consumable energy for extracting gases in engineering equipment.
United States Economic Sanctions on Iran and Their Impacts on the Middle Eastern Art Market
Authors: Rafiei Vardanjani Ahmad
Published: December 2020

The United States’ sanctions on Iran have limited the Iranian art market’s connections with the international art network. Galleries try to compensate for such limitations through online marketing and exhibition. Thus, the sanctions not only impact the form of marketing exerted by dealers but also directly influence the type of artistic production. Such changes also reshape the art market in the Arab states. The transition from tangible to intangible has become a strategy for the regional market to bypass the sanctions and develop business with the global collectors and institutions. A quantitative analysis was used to demonstrate the impact of the sanctions on the art market in Iran and the United Arab Emirates. This analysis examined all exhibitions in 12 commercial galleries in Tehran and Dubai from 2009 to 2019, statistically assessing the index of changes over this period and calculating the variations, particularly during the years of intensified sanctions. The study indicates how the propensity of galleries for a digitally networked economy is becoming a solution to reduce the impacts of the sanctions in order for the galleries to maintain their clientele of international collectors and dealers.
Vertical Profiles of Atmospheric Species Concentrations and Nighttime Boundary Layer Structure in the Dry Season over an Urban Environment in Central Amazon Collected by an Unmanned Aerial Vehicle
Authors: Guimarães Patrícia , Ye Jianhuai , Batista Carla , Barbosa Rafael , Ribeiro Igor , Medeiros Adan , Zhao Tianning , Hwang Wei-Chun , Hung Hui-Ming , Souza Rodrigo , T. Martin Scot
Published: December 2020

Nighttime vertical profiles of ozone, PM2.5 and PM10 particulate matter, carbon monoxide, temperature, and humidity were collected by a copter-type unmanned aerial vehicle (UAV) over the city of Manaus, Brazil, in central Amazon during the dry season of 2018. The vertical profiles were analyzed to understand the structure of the urban nighttime boundary layer (NBL) and pollution within it. The ozone concentration, temperature, and humidity had an inflection between 225 and 350 m on most nights, representing the top of the urban NBL. The profile of carbon monoxide concentration correlated well with the local evening vehicular congestion of a modern transportation fleet, providing insight into the surface-atmosphere dynamics. In contrast, events of elevated PM2.5 and PM10 concentrations were not explained well by local urban emissions, but rather by back trajectories that intersected regional biomass burning. These results highlight the potential of the emerging technologies of sensor payloads on UAVs to provide new constraints and insights for understanding the pollution dynamics in nighttime boundary layers in urban regions.
Comparative Analysis of the Transcriptome, Proteome, and miRNA Profile of Kupffer Cells and Monocytes
Authors: Elchaninov Andrey , Lokhonina Anastasia , Nikitina Maria , Vishnyakova Polina , Makarov Andrey , Arutyunyan Irina , Poltavets Anastasiya , Kananykhina Evgenia , Kovalchuk Sergey , Karpulevich Evgeny , Bolshakova Galina , Sukhikh Gennady , Fatkhudinov Timur
Published: December 2020

Macrophage populations in most mammalian organs consist of cells of different origin. Resident macrophages originate from erythromyeloid precursors of the yolk sac wall; maintenance of the numbers of such macrophages in postnatal ontogenesis is practically independent of bone marrow haematopoiesis. The largest populations of the resident macrophages of embryonic origin are found in the central nervous system (microglia) and liver (Kupffer cells). In contrast, skin dermis and mucous membranes become predominantly colonized by bone marrow-derived monocytes that show pronounced functional and phenotypic plasticity. In the present study, we compared Kupffer cells and monocytes using the immunophenotype, gene expression profile, proteome, and pool of microRNA. The observed differences did not consider the resident liver macrophages as purely M2 macrophages or state that monocytes have purely M1 features. Monocytes show signs of high plasticity and sensitivity to pathogen-associated molecular patterns (e.g., high levels of transcription for Tlr 2, 4, 7, and 8). In contrast, the resident liver macrophages were clearly involved in the regulation of specific organ functions (nitrogen metabolism, complement system protein synthesis).
3D-Hepatocyte Culture Applied to Parasitology: Immune Activation of Canine Hepatic Spheroids Exposed to Leishmania infantum
Authors: Rodrigues Armanda V. , Alexandre-Pires Graça , Valério-Bolas Ana , Santos-Mateus David , Rafael-Fernandes Mariana , Pereira Maria A. , Ligeiro Dário , Nunes Telmo , Alves-Azevedo Raquel , Santos Marcos , Pereira da Fonseca Isabel , Santos-Gomes Gabriela
Published: December 2020

The application of innovative three-dimensional (3D) spheroids cell culture strategy to Parasitology offers the opportunity to closely explore host–parasite interactions. Here we present a first report on the application of 3D hepatic spheroids to unravel the immune response of canine hepatocytes exposed to Leishmania infantum. The liver, usually considered a major metabolic organ, also performs several important immunological functions and constitutes a target organ for L. infantum infection, the etiological agent of canine leishmaniasis (CanL), and a parasitic disease of major veterinary and public health concern. 3D hepatic spheroids were able to sense and immunologically react to L. infantum parasites, generating an innate immune response by increasing nitric oxide (NO) production and enhancing toll-like receptor (TLR) 2 and interleukin-10 gene expression. The immune response orchestrated by canine hepatocytes also lead to the impairment of several cytochrome P450 (CYP450) with possible implications for liver natural xenobiotic metabolization capacity. The application of meglumine antimoniate (MgA) increased the inflammatory response of 3D hepatic spheroids by inducing the expression of Nucleotide oligomerization domain (NOD) -like receptors 1 and NOD2 and TLR2, TLR4, and TLR9 and enhancing gene expression of tumour necrosis factor α. It is therefore suggested that hepatocytes are key effector cells and can activate and orchestrate the immune response to L. infantum parasites.
The Prognostic Role of Lactate Concentrations after Aneurysmal Subarachnoid Hemorrhage
Authors: Ndieugnou Djangang Narcisse , Ramunno Pamela , Izzi Antonio , Garufi Alessandra , Menozzi Marco , Diaferia Daniela , Peluso Lorenzo , Prezioso Chiara , Talamonti Marta , Njimi Hassane , Schuind Sophie , Vincent Jean-Louis , Creteur Jacques , Taccone Fabio , Gouvea Bogossian Elisa
Published: December 2020

Blood lactate concentrations are often used to assess global tissue perfusion in critically ill patients; however, there are scarce data on lactate concentrations after subarachnoid hemorrhage (SAH). We aimed to assess the prognostic role of serial blood lactate measurements on hospital mortality and neurological outcomes at 3 months after SAH. We reviewed all SAH patients admitted to the intensive care unit from 2007 to 2019 and recorded the highest daily arterial lactate concentration for the first 6 days. Patients with no lactate concentration were excluded. Hyperlactatemia was defined as a blood lactate concentration >2.0 mmol/L. A total of 456 patients were included: 158 (35%) patients died in hospital and 209 (46%) had an unfavorable outcome (UO) at 3 months. The median highest lactate concentration was 2.7 (1.8–3.9) mmol/L. Non-survivors and patients with UO had significantly higher lactate concentrations compared to other patients. Hyperlactatemia increased the chance of dying (OR 4.19 (95% CI 2.38–7.39)) and of having UO in 3 months (OR 4.16 (95% CI 2.52–6.88)) after adjusting for confounding factors. Therefore, initial blood lactate concentrations have prognostic implications in patients with SAH; their role in conjunction with other prognostic indicators should be evaluated in prospective studies.
Next Generation Sequencing-Based Profiling of Cell Free DNA in Patients with Advanced Non-Small Cell Lung Cancer: Advantages and Pitfalls
Authors: Esposito Abate Riziero , Frezzetti Daniela , Maiello Monica Rosaria , Gallo Marianna , Camerlingo Rosa , De Luca Antonella , De Cecio Rossella , Morabito Alessandro , Normanno Nicola
Published: December 2020

Lung cancer (LC) is the main cause of death for cancer worldwide and non-small cell lung cancer (NSCLC) represents the most common histology. The discovery of genomic alterations in driver genes that offer the possibility of therapeutic intervention has completely changed the approach to the diagnosis and therapy of advanced NSCLC patients, and tumor molecular profiling has become mandatory for the choice of the most appropriate therapeutic strategy. However, in approximately 30% of NSCLC patients tumor tissue is inadequate for biomarker analysis. The development of highly sensitive next generation sequencing (NGS) technologies for the analysis of circulating cell-free DNA (cfDNA) is emerging as a valuable alternative to assess tumor molecular landscape in case of tissue unavailability. Additionally, cfDNA NGS testing can better recapitulate NSCLC heterogeneity as compared with tissue testing. In this review we describe the main advantages and limits of using NGS-based cfDNA analysis to guide the therapeutic decision-making process in advanced NSCLC patients, to monitor the response to therapy and to identify mechanisms of resistance early. Therefore, we provide evidence that the implementation of cfDNA NGS testing in clinical research and in the clinical practice can significantly improve precision medicine approaches in patients with advanced NSCLC.
Pediatric Rhabdomyosarcomas: Three-Dimensional Radiological Assessments after Induction Chemotherapy Predict Survival Better than One-Dimensional and Two-Dimensional Measurements
Authors: Orsatti Giovanna , Morosi Carlo , Giraudo Chiara , Varotto Alessia , Crimì Filippo , Bonzini Miriam , Minotti Marta , Frigo Anna Chiara , Zanetti Ilaria , Chiaravalli Stefano , Casanova Michela , Ferrari Andrea , Bisogno Gianni , Stramare Roberto
Published: December 2020

Radiological response to neoadjuvant chemotherapy is currently used to assess the efficacy of treatment in pediatric patients with rhabdomyosarcoma (RMS), but the association between early tumor response on imaging and survival is still controversial. The aim of this study was to investigate the prognostic value of assessing radiological response after induction therapy in pediatric RMS, comparing four different methods. This retrospective, two-center study was conducted on 66 non-metastatic RMS patients. Two radiologists measured tumor size on pre- and post-treatment magnetic resonance (MR) or computed tomography (CT) images using four methods: considering maximal diameter with the 1D-RECIST (Response Evaluation Criteria in Solid Tumors); multiplying the two maximal diameters with the 2D-WHO (World Health Organization); multiplying the three maximal diameters with the 3D-EpSSG (European pediatric Soft tissue sarcoma Study Group); obtaining a software-assisted volume assessment with the 3D-Osirix. Each patient was classified as a responder or non-responder based on the proposed thresholds for each method. Tumor response was compared with survival using Kaplan–Meier plots, the log-rank test, and Cox’s regression. Agreement between methods and observers (weighted-κ) was also calculated. The 5-year event-free survival (5yr-EFS) calculated with the Kaplan–Meier plots was significantly longer for responders than for non-responders with all the methods, but the 3D assessments differentiated between the two groups better than the 1D-RECIST or 2D-WHO (p1D-RECIST = 0.018, p2D-WHO = 0.007, p3D-EpSSG and p3D-Osirix < 0.0001). Comparing the 5yr-EFS of responders and non-responders also produced adjusted hazard ratios of 3.57 (p = 0.0158) for the 1D-RECIST, 5.05 for the 2D-WHO (p = 0.0042), 14.40 for the 3D-EpSSG (p < 0.0001) and 11.60 for the 3D-Osirix (p < 0.0001), indicating that the volumetric measurements were significantly more strongly associated with EFS. Inter-method agreement was excellent between the 3D-EpSSG and the 3D-Osirix (κ = 0.98), and moderate for the other comparisons (0.5 < κ < 0.8). The 1D-RECIST and the 2D-WHO tended to underestimate response to treatment. Inter-observer agreement was excellent with all methods (κ > 0.8) except for the 2D-WHO (κ = 0.7). In conclusion, early tumor response was confirmed as a significant prognostic factor in RMS, and the 3D-EpSSG and 3D-Osirix methods predicted response to treatment better than the 1D-RECIST or 2D-WHO measurements.
Effect of Body Weight and Other Metabolic Factors on Risk of Non-Small Cell Lung Cancer among Veterans with HIV and a History of Smoking
Authors: Garcia Jose M. , Kramer Jennifer R. , Richardson Peter A. , Ahmed Sarah , Royse Kathryn E. , White Donna L. , Raychaudhury Suchismita , Chang Elaine , Hartman Christine M. , Silverberg Michael J. , Chiao Elizabeth Y.
Published: December 2020

Among people living with HIV (PWH), there has been an increasing incidence of non-small cell lung cancer (NSCLC) and metabolic abnormalities, including dyslipidemia, which can modulate NSCLC risk. In this article, we evaluate which metabolic risk factors are associated with incident risk among PWH who smoke. This is done through a retrospective cohort study, using data of HIV+ veterans who smoke from the nationwide Veterans Affairs (VA) healthcare system. Data on diagnostic codes, medication, and laboratory values of 33,351 veterans were obtained using the VA’s Corporate Data Warehouse and Central Cancer Registry. We calculated NSCLC incidence and utilized Cox regression to determine metabolic factors associated with NSCLC risk. HIV+ cohort was 97.4% male; median age = 47 years and 20,050 (60.1%) well-controlled (≥80% follow-up time undetectable viral load). Crude incidence rates were lower in well-controlled PWH (1.46 vs. 2.06/1000 PY). Metabolic factors associated with incident NSCLC risk included lower BMI at HIV diagnosis and cachexia history in both groups, while HDL and triglycerides were significant in non-well-controlled smokers only. Our findings that lower BMI at HIV diagnosis, history of cachexia among individuals with well-controlled HIV, and cachexia presence at diagnosis are associated with increased risk of developing NSCLC in PWH with a history of smoking have important implications.
The Paradoxical Effect of PARP Inhibitor BGP-15 on Irinotecan-Induced Cachexia and Skeletal Muscle Dysfunction
Authors: Campelj Dean G. , Timpani Cara A. , Petersen Aaron C. , Hayes Alan , Goodman Craig A. , Rybalka Emma
Published: December 2020

Chemotherapy-induced muscle wasting and dysfunction is a contributing factor to cachexia alongside cancer and increases the risk of morbidity and mortality. Here, we investigate the effects of the chemotherapeutic agent irinotecan (IRI) on skeletal muscle mass and function and whether BGP-15 (a poly-(ADP-ribose) polymerase-1 (PARP-1) inhibitor and heat shock protein co-inducer) adjuvant therapy could protect against IRI-induced skeletal myopathy. Healthy 6-week-old male Balb/C mice (n = 24; 8/group) were treated with six intraperitoneal injections of either vehicle, IRI (30 mg/kg) or BGP-15 adjuvant therapy (IRI+BGP; 15 mg/kg) over two weeks. IRI reduced lean and tibialis anterior mass, which were attenuated by IRI+BGP treatment. Remarkably, IRI reduced muscle protein synthesis, while IRI+BGP reduced protein synthesis further. These changes occurred in the absence of a change in crude markers of mammalian/mechanistic target of rapamycin (mTOR) Complex 1 (mTORC1) signaling and protein degradation. Interestingly, the cytoskeletal protein dystrophin was reduced in both IRI- and IRI+BGP-treated mice, while IRI+BGP treatment also decreased β-dystroglycan, suggesting significant remodeling of the cytoskeleton. IRI reduced absolute force production of the soleus and extensor digitorum longus (EDL) muscles, while IRI+BGP rescued absolute force production of the soleus and strongly trended to rescue force output of the EDL (p = 0.06), which was associated with improvements in mass. During the fatiguing stimulation, IRI+BGP-treated EDL muscles were somewhat susceptible to rupture at the musculotendinous junction, likely due to BGP-15’s capacity to maintain the rate of force development within a weakened environment characterized by significant structural remodeling. Our paradoxical data highlight that BGP-15 has some therapeutic advantage by attenuating IRI-induced skeletal myopathy; however, its effects on the remodeling of the cytoskeleton and extracellular matrix, which appear to make fast-twitch muscles more prone to tearing during contraction, could suggest the induction of muscular dystrophy and, thus, require further characterization.
Tumors Widely Express Hundreds of Embryonic Germline Genes
Authors: Bruggeman Jan Willem , Irie Naoko , Lodder Paul , van Pelt Ans M. M. , Koster Jan , Hamer Geert
Published: December 2020

We have recently described a class of 756 genes that are widely expressed in cancers, but are normally restricted to adult germ cells, referred to as germ cell cancer genes (GC genes). We hypothesized that carcinogenesis involves the reactivation of biomolecular processes and regulatory mechanisms that, under normal circumstances, are restricted to germline development. This would imply that cancer cells share gene expression profiles with primordial germ cells (PGCs). We therefore compared the transcriptomes of human PGCs (hPGCs) and PGC-like cells (PGCLCs) with 17,382 samples from 54 healthy somatic tissues (GTEx) and 11,003 samples from 33 tumor types (TCGA), and identified 672 GC genes, expanding the known GC gene pool by 387 genes (51%). We found that GC genes are expressed in clusters that are often expressed in multiple tumor types. Moreover, the amount of GC gene expression correlates with poor survival in patients with lung adenocarcinoma. As GC genes specific to the embryonic germline are not expressed in any adult tissue, targeting these in cancer treatment may result in fewer side effects than targeting conventional cancer/testis (CT) or GC genes and may preserve fertility. We anticipate that our extended GC dataset enables improved understanding of tumor development and may provide multiple novel targets for cancer treatment development.
MiR-181 Family Modulates Osteopontin in Glioblastoma Multiforme
Authors: Marisetty Anantha , Wei Jun , Kong Ling-Yuan , Ott Martina , Fang Dexing , Sabbagh Aria , Heimberger Amy B.
Published: December 2020

MiRNAs can silence a wide range of genes, which may be an advantage for targeting heterogenous tumors like glioblastoma. Osteopontin (OPN) plays both an oncogenic role in a variety of cancers and can immune modulate macrophages. We conducted a genome wide profiling and bioinformatic analysis to identify miR-181a/b/c/d as potential miRNAs that target OPN. Luciferase assays confirmed the binding potential of miRNAs to OPN. Expression levels of miR-181a/b/c/d and OPN were evaluated by using quantitative real-time PCR and enzyme-linked immunosorbent assay in mouse and human glioblastomas and macrophages that showed these miRNAs were downregulated in Glioblastoma associated CD11b+ cells compared to their matched blood CD14b+ cells. miRNA mimicking and overexpression using lentiviruses showed that MiR-181a overexpression in glioblastoma cells led to decreased OPN production and proliferation and increased apoptosis in vitro. MiR-181a treatment of immune competent mice bearing intracranial glioblastoma demonstrated a 22% increase in median survival duration relative to that of control mice.
Effect of Radiotherapy in Addition to Surgery in Early Stage Endometrial Cancer: A Population-Based Study
Authors: Medenwald Daniel , Langer Susan , Gottschick Cornelia , Vordermark Dirk
Published: December 2020

Background: The role of radiotherapy in the management of early (FIGO I) endometrial cancer is controversial with limited availability of prospective data from randomized trials. Methods: German Epidemiologic Cancer Registries provided by the Robert Koch Institute. We considered FIGO I cases with recorded operative treatment (n = 12,718, 2000–2017). We computed hazard ratios (HR) from relative survival models in relation to the mortality of the general population with 95% confidence intervals (CI). Multivariate models were adjusted for age, stage (IA vs. IB), grading, and chemotherapy. Radiotherapy included external radiotherapy and brachytherapy. Results: Cases with a favorable risk profile (FIGO IA, G1/G2) had a slightly lower survival rate, relative to the general population (FIGO IA: 0.9, G1: 0.91). The proportion of FIGO IA cases was lower in the radiotherapy group (52.6%) vs. cases without radiotherapy (78.6%). Additional treatment with radiotherapy was beneficial in FIGO IB (HR = 0.74) and all histopathological grades, but not FIGO IA cases (HR = 0.93) cases. Compared to IA tumors, IB cases had a HR of 1.51 (95% CI: 1.34–1.7). Conclusions: Radiotherapy in addition to surgery is beneficial for patients in a FIGO IB stage. Further studies need to address the impact of new techniques and risk assessment.
B7-H3 Chimeric Antigen Receptor Redirected T Cells Target Anaplastic Lymphoma Kinase-Positive Anaplastic Large Cell Lymphoma
Authors: Zi Zhenguo , Zhao Haihong , Wang Huanyu , Ma Xiaojing , Wei Fang
Published: December 2020

Potent CAR-T therapies that target appropriate antigens can benefit the treatment of anaplastic lymphoma kinase-positive (ALK+) anaplastic large cell lymphoma (ALCL), which is the most common subtype of T cell lymphoma. In this study, we observed overexpression of B7-H3 in ALCL cell lines derived from clinical samples and differential expression of B7-H3 in an ALK-induced T cell transformation model. A B7-H3-redirected CAR based on scFv from mAb 376.96 was developed. B7-H3 CAR-T cells showed strong cytotoxicity and cytokine secretion against target ALCL cells (SUP-M2, SU-DHL-1, and Karpas 299) in vitro. Furthermore, the B7-H3 CAR-T cells exhibited proliferative capacity and a memory phenotype upon repeated antigen stimulation. We demonstrated that B7-H3 CAR-T cells could promptly eradicate ALCL in murine xenografts. Taken together, B7-H3 is a novel and promising target in ALCLs and B7-H3 CAR-T may be a viable treatment option for ALCL.
Machine Learning Algorithms to Predict Recurrence within 10 Years after Breast Cancer Surgery: A Prospective Cohort Study
Authors: Lou Shi-Jer , Hou Ming-Feng , Chang Hong-Tai , Chiu Chong-Chi , Lee Hao-Hsien , Yeh Shu-Chuan Jennifer , Shi Hon-Yi
Published: December 2020

No studies have discussed machine learning algorithms to predict recurrence within 10 years after breast cancer surgery. This study purposed to compare the accuracy of forecasting models to predict recurrence within 10 years after breast cancer surgery and to identify significant predictors of recurrence. Registry data for breast cancer surgery patients were allocated to a training dataset (n = 798) for model development, a testing dataset (n = 171) for internal validation, and a validating dataset (n = 171) for external validation. Global sensitivity analysis was then performed to evaluate the significance of the selected predictors. Demographic characteristics, clinical characteristics, quality of care, and preoperative quality of life were significantly associated with recurrence within 10 years after breast cancer surgery (p < 0.05). Artificial neural networks had the highest prediction performance indices. Additionally, the surgeon volume was the best predictor of recurrence within 10 years after breast cancer surgery, followed by hospital volume and tumor stage. Accurate recurrence within 10 years prediction by machine learning algorithms may improve precision in managing patients after breast cancer surgery and improve understanding of risk factors for recurrence within 10 years after breast cancer surgery.
Establishment of a Mouse Ovarian Cancer and Peritoneal Metastasis Model to Study Intraperitoneal Chemotherapy
Authors: Rezniczek Günther A. , Buggisch Jonathan , Sobilo Julien , Launay Alexandre , Lerondel Stéphanie , Le Pape Alain , Ouaissi Mehdi , Göhler Daniel , Senkal Metin , Giger-Pabst Urs , Tempfer Clemens B.
Published: December 2020

Intraperitoneal chemotherapy (IPC) is a locoregional treatment option in patients with peritoneal metastases (PM). Here, we present an ovarian cancer (OC)-derived PM mouse model for the study of different forms of IPC. Xenograft cell proliferation (luciferase-transfected OVCAR3 and SKOV3 clones) and growth kinetics were assessed using PET scan, bioluminescence imaging (BLI), and histological tumor analysis. Liquid IPC was achieved by intraperitoneal injection with/without capnoperitoneum (6–7 mmHg). Pressurized intraperitoneal aerosol chemotherapy (PIPAC) was mimicked using an intratracheal drug aerosol administration system (micro-nozzle), which, as demonstrated by ex vivo granulometric analysis using laser diffraction spectrometry, produced a polydisperse, bimodal aerosol with a volume-weighted median diameter of (26.49 ± 2.76) µm. Distribution of Tc-99m-labeled doxorubicin in mice was characterized using SPECT and was dependent on the delivery mode and most homogeneous when the micro-nozzle was used. A total of 2 mg doxorubicin per kg body weight was determined to be the optimally effective and tolerable dose to achieve at least 50% tumor reduction. Repeated PIPAC (four times at seven-day-intervals) with doxorubicin in SKOV3-luc tumor-bearing mice resulted in halted tumor proliferation and tumor load reduced after the second round of PIPAC versus controls and the number of tumor nodules was significantly reduced (27.7 ± 9.5 vs. 57.3 ± 9.5; p = 0.0003). Thus, we established the first mouse model of OC PM for the study of IPC using a human xenograft with SKOV3 cells and an experimental IPC setup with a miniaturized nozzle. Repeated IPC was feasible and demonstrated time-dependent anti-tumor activity.
Fluorescent Silica Hybrid Film-Forming Materials Based on Salicylaldazine
Authors: Raditoiu Alina , Raditoiu Valentin , Raduly Florentina Monica , Ispas Georgiana Cornelia , Purcar Violeta , Frone Adriana Nicoleta , Manea Raluca , Wagner Luminita Eugenia , Anastasescu Mihai
Published: December 2020

Fluorescent film-forming materials were obtained by embedding salicylaldazine (SAA) in silica hybrids generated by sol–gel processes from different silane precursors in acid catalysis. Tuned local environments for the fluorophore were generated in the hosting network by modifying silica sols with organic groups through the co-condensation of tetraethylortosilicate (TEOS) and different alkoxysilanes hydrolysis products. The photophysical properties of the luminescent hybrid films were studied in direct relationship with structural, textural, and surface properties and based on interactions between SAA species and the silica hosting network. Film-forming materials were studied in order to determine differences in absorption and fluorescence emission due to the environments around the fluorophore. The variations recorded in the fluorescence emission spectra of the hybrid films were related to interactions established between the fluorophore species and their sterically hindered surroundings of the host hybrid silica, where free molecular motions are restricted. The influence of the type and amount of network modifier and of the fluorophore loading on the transparency of the films and fluorescence intensity was also investigated. The study carried out led to the elucidation of the necessary conditions for obtaining luminescent film-forming materials with high luminescence intensity and transparency useful for the design of new light concentrators.
Study of Texture Analysis on Asymmetric Cryorolled and Annealed CoCrNi Medium Entropy Alloy
Authors: Wu Yuze , Liu Juan , Bhatta Laxman , Kong Charlie , Yu Hailiang
Published: December 2020

CoCrNi equiatomic medium entropy alloy sheets were prepared by asymmetric rolling, cryorolling, and asymmetric cryorolling. The asymmetric cryorolled samples exhibited a noteworthy ultra-fine-grain heterogeneous lamella structure. The microstructure and corresponding hardness obtained by different rolling processes and subsequent annealing are compared. It can be seen from the results that the cryogenic deformation temperature had a stronger effect on the mechanical properties of the medium entropy alloys (MEA), compared with the shear strain caused by the asymmetric cryorolling. The effect of annealing temperature on texture components and volume fractions of the specially rolled samples was also analyzed. The result revealed that the recrystallized MEA exhibited similar texture components and the corresponding volume fraction, which indicated that the rolling process had limited influence on the formation of annealing texture. The recrystallized texture after annealing retained the deformation texture and twin related orientations appeared. Asymmetric rolled MEA showed strong random composition than symmetric rolled MEA regardless of rolling temperature. The recrystallized textures of the species obtained by the three rolling processes did not exhibit a significant dependence on the annealing temperature.
Development of Novel SNP Assays for Genetic Analysis of Rare Minnow (Gobiocypris rarus) in a Successive Generation Closed Colony
Authors: Cai Lei , Hou Miaomiao , Xu Chunsen , Xia Zhijun , Wang Jianwei
Published: December 2020

The complex genetic architecture of closed colonies during successive passages poses a significant challenge in the understanding of the genetic background. Research on the dynamic changes in genetic structure for the establishment of a new closed colony is limited. In this study, we developed 51 single nucleotide polymorphism (SNP) markers for the rare minnow (Gobiocypris rarus) and conducted genetic diversity and structure analyses in five successive generations of a closed colony using 20 SNPs. The range of mean Ho and He in five generations was 0.4547–0.4983 and 0.4445–0.4644, respectively. No significant differences were observed in the Ne, Ho, and He (p > 0.05) between the five closed colony generations, indicating well-maintained heterozygosity. The F-statistics analysis revealed a relatively stable genetic structure of the closed colony. Furthermore, the genetic distance between the newer and older generations increased with the breeding generations in closed colonies. Our results confirmed previous findings in the same samples using microsatellite markers. The results will be beneficial for establishing genetic variability monitoring criteria and restoration of the wild population of the rare minnow and other laboratory fish.
Testing Bathymetric and Regional Patterns in the Southwest Atlantic Deep Sea Using Infaunal Diversity, Structure, and Function
Authors: dos Santos Giovanni A. P. , Silva Alexsandra C. , Esteves André M. , Ribeiro-Ferreira Venina P. , Neres Patricia. F. , Valdes Yirina , Ingels Jeroen
Published: December 2020

A better understanding of deep-sea biology requires knowledge of the structure and function of their communities, the spatial, temporal, and environmental patterns, and the changes and dynamics that govern them. Some of the most studied patterns in deep-sea biology are those related to bathymetrical gradients. For meiofauna and nematodes, such studies have highlighted the importance of recognizing regional differences in using ecological mechanisms to explain those patterns. Despite holding significant fisheries and oil and gas resources, the eastern Brazilian Continental Margin is poorly understood with respect to its seafloor biology and ecology. To answer ecological questions of deep-sea infaunal structural and functional diversity in relation to bathymetrical patterns, we used nematode data from five bathymetric transects (400, 1000, 1900, 2500, and 3000 m water depth) sampled in 2011 and 2013 on the Espírito Santo slope off the coast of southeast (SE) Brazil. Deep nematode community analysis based on 6763 nematode identifications showed very high levels of diversity (201 genera; 43 families) compared to other ocean basins and deep-sea regions. Our analyses showed that there is a distinct bathymetric break in standing stocks and community structure between 1000 and 1900 m. Nematode standing stocks were much higher at 400 and 1000 m compared to those for similar depths worldwide, likely linked to the intense and frequent upwelling and specific hydrographic and topographic identity of the region. The bathymetric break was not present for structural and functional nematode diversity. Instead, bathymetric regressions showed that they increased gradually toward 3000 m water depth. The deep Espírito Santo basin is characterized by rich and equitable nematode communities that are both mature and trophically diverse. General deep-sea ecological theories apply to our findings, but there are also substantial regional effects related to the local margin topography, upwelling, and oceanographic and hydrodynamic processes that make the Espírito Santo Basin a unique and diverse deep-sea ecosystem.
Genetic Diversity and Population Structure of the Spring Orchid Cymbidium goeringii in Korean Distant Islands
Authors: Noh Seung Woo , Park Jun-Kyu , Yu Jin Seok , Nam Da Eun , Do Yuno , Chung Ki Wha
Published: December 2020

The spring orchid (Cymbidium goeringii), found in northeast Asia, is one of the most popular and horticulturally important species of the orchid family. This study analyzed the genetic diversity and population structure of the spring orchid populations in the small islands and mainland South Korea using 11 microsatellite markers. The genetic diversities of spring orchid populations in the distant islands (Heuksan Island and Ulleung Island) were slightly lower than that of the mainland population (Yeonggwang-gun). The population structure in the mainland was genetically separated from the populations in the islands. The population of Ulleung Island, located in the eastern part of the Korean peninsula, was genetically closer to the populations from China and Japan than to the populations from Yeonggwang-gun and Heuksan Island, which are geographically close to China. These results imply that the populations of spring orchids distributed in Yeonggwang-gun and Heuksan Island appear not to be influenced by the yellow dust winds. As the first population genetic study of spring orchids distributed in small distant islands, our study will be useful for understanding the genetic diversity and population structure of isolated C. goeringii populations.
COPD Imaging on a 3rd Generation Dual-Source CT: Acquisition of Paired Inspiratory-Expiratory Chest Scans at an Overall Reduced Radiation Risk
Authors: Gawlitza Joshua , Henzler Thomas , Trinkmann Frederik , Nekolla Elke , Haubenreisser Holger , Brix Gunnar
Published: December 2020

As stated by the Fleischner Society, an additional computed tomography (CT) scan in expiration is beneficial in patients with chronic obstructive pulmonary disease (COPD). It was thus the aim of this study to evaluate the radiation risk of a state-of-the-art paired inspiratory-expiratory chest scan compared to inspiration-only examinations. Radiation doses to 28 organs were determined for 824 COPD patients undergoing routine chest examinations at three different CT systems–a conventional multi-slice CT (MSCT), a 2nd generation (2nd-DSCT), and 3rd generation dual-source CT (3rd-DSCT). Patients examined at the 3rd-DSCT received a paired inspiratory-expiratory scan. Organ doses, effective doses, and lifetime attributable cancer risks (LAR) were calculated. All organ and effective doses were significantly lower for the paired inspiratory-expiratory protocol (effective doses: 4.3 ± 1.5 mSv (MSCT), 3.0 ± 1.2 mSv (2nd-DSCT), and 2.0 ± 0.8 mSv (3rd-DSCT)). Accordingly, LAR was lowest for the paired protocol with an estimate of 0.025 % and 0.013% for female and male patients (50 years) respectively. Image quality was not compromised. Paired inspiratory-expiratory scans can be acquired on 3rd-DSCT systems at substantially lower dose and risk levels when compared to inspiration-only scans at conventional CT systems, offering promising prospects for improved COPD diagnosis.
Volitional Swimming Kinematics of Blacktip Sharks, Carcharhinus limbatus, in the Wild
Authors: Porter Marianne E. , Ruddy Braden T. , Kajiura Stephen M.
Published: December 2020

Recent work showed that two species of hammerhead sharks operated as a double oscillating system, where frequency and amplitude differed in the anterior and posterior parts of the body. We hypothesized that a double oscillating system would be present in a large, volitionally swimming, conventionally shaped carcharhinid shark. Swimming kinematics analyses provide quantification to mechanistically examine swimming within and among species. Here, we quantify blacktip shark (Carcharhinus limbatus) volitional swimming kinematics under natural conditions to assess variation between anterior and posterior body regions and demonstrate the presence of a double oscillating system. We captured footage of 80 individual blacktips swimming in the wild using a DJI Phantom 4 Pro aerial drone. The widespread accessibility of aerial drone technology has allowed for greater observation of wild marine megafauna. We used Loggerpro motion tracking software to track five anatomical landmarks frame by frame to calculate tailbeat frequency, tailbeat amplitude, speed, and anterior/posterior variables: amplitude and frequency of the head and tail, and the body curvature measured as anterior and posterior flexion. We found significant increases in tailbeat frequency and amplitude with increasing swimming speed. Tailbeat frequency decreased and tailbeat amplitude increased as posterior flexion amplitude increased. We found significant differences between anterior and posterior amplitudes and frequencies, suggesting a double oscillating modality of wave propagation. These data support previous work that hypothesized the importance of a double oscillating system for increased sensory perception. These methods demonstrate the utility of quantifying swimming kinematics of wild animals through direct observation, with the potential to apply a biomechanical perspective to movement ecology paradigms.
Optimization of the Ablative Laser Cutting of Shadow Mask for Organic FET Electrode Fabrication
Authors: Tomczyk Mariusz , Kubik Paweł , Waliszewski Witold
Published: December 2020

This article presents an ablative method of cutting masks from ultra-thin metal foils using nanosecond laser pulses. As a source of laser radiation, a pulsed fiber laser with a wavelength of 1062 nm with the duration of pulses from 15 to 220 nanoseconds (ns), was used in the research. The masks were made of stainless-steel foil with thicknesses of 30 µm, 35 µm, and 120 µm. Channels of different lengths from 50 to 300 µm were tested. The possibilities and limitations of the presented method are described. The optimization of the cutting process parameters was performed using the experiment planning techniques. A static, determined complete two-level plan (SP/DC 24) was used. On the basis of the analysis of the test structures, we designed and produced precise shading masks used in the process of organic field effect transistor (OFET) electrode evaporation. The ablative method proved suitable to produce masks with canals of minimum lengths of 70 µm. It offers facile, fast, and economically viable shadow mask fabrication for organic electronics applications, which moreover might enable fast prototyping and circuit design.
Humanity Can Still Stop Climate Change by Implementing a New International Climate Agreement and Applying Radical New Technology
Authors: Glasnovic Zvonimir , Margeta Karmen , Logar Nataša Zabukovec
Published: December 2020

There is a broad consensus worldwide that anthropogenic climate change is a scientific fact. Likewise, the fact is that the UN’s efforts to address climate change over the last 28 years have not been successful enough. It is evident that the global average temperature is on the rise (1.1 °C above pre-industrial levels in 2019). A particular concern comes from the fact that the Paris Agreement on keeping increases in the global average temperature to below +2 °C is an unenforceable ambition, since the focus is more on consequences than causes. In addition, economic policies regarding global taxes, as well as adaptation and mitigation measures, are questionable, as there is no evidence that changes in the climate system will proceed at the same rate in the coming years. This paper proposes an engineering approach that considers all relevant aspects of the climate change problem and proposes a new policy, named the “Climate New Deal”. It deals with: (i) Reorientation from a high-carbon economy to a green economy; (ii) The intensive use of radically new technology, e.g., “Seawater Steam Engine” technology for the simultaneous production of thermal and electric energy and drinking water; and (iii) The intensive use of energy efficient technologies and RES technologies, especially in transport.
Experimental Investigation of the Seamless Gearshift Mechanism Using an Electric Motor and a Planetary Gear-Set
Authors: Kim Sun Je , Kim Kyung-Soo
Published: December 2020

Vehicle transmission which has discrete gear-stages inevitably produces torque drop during shifting gears. This torque drop should be minimized because it may lead to uncomfortable driving feeling and degradation of acceleration performance. In accordance with the spread of electric-powered vehicle technology, this study proposes novel transmission architecture to eliminate torque drop during shifting gears by using one electric motor and verifies its operating concept through experiments with a test-bench. The proposed transmission, called CGST (clutchless geared smart transmission) can synchronize the gear-shaft to be engaged for the next gear-stage with the output shaft by using a planetary gear-set and an electric motor. The CGST has a dual input gear-box with even and odd gear-stages on different input shafts, and the planetary gear-set and the electric motor control the speeds of each input shafts to smoothly engage the next gear-stage. This idea was verified by the simplified test-rig in this study. Three distinct scenarios for gear-shift including starting from engine idling, odd to even gear-shift, and even to odd gear-shift were conducted in the experiment. The shifting performance of the CGST was evaluated by comparing it with the results of the manual transmission (MT). As a result, the CGST shows only 24% of torque drop of the MT, and torque oscillation followed after gear-shifting is reduced by 26%. Although the developed test bench was of limited size, the possibility and expected performance of the CGST have been confirmed as the solution for seamless transmission.
Correction: Branca, G., et al. Forest Protection Unifies, Silviculture Divides: A Sociological Analysis of Local Stakeholders’ Voices after Coppicing in the Marganai Forest (Sardinia, Italy). Forests 2020, 11, 708
Authors: Branca Giampiero , Piredda Irene , Scotti Roberto , Chessa Laura , Murgia Ilenia , Ganga Antonio , Campus Sergio Francesco , Lovreglio Raffaella , Guastini Enrico , Schwarz Massimiliano , Giadrossich Filippo
Published: December 2020

We have recently been made aware by the Forests Editorial Offices of some errors and omissions in the Introduction Section 1 [...]