bluuebunny/crossref_metadata_2025 huggingface dataset:
A Guide to Making Highly Reproducible Li-Ion Single-Layer Pouch Cells for Academic Researchers
Authors: Garayt Matthew D. L. , Johnson Michel B. , Laidlaw Lauren , McArthur Mark A. , Trussler Simon , Harlow Jessie E. , Dahn J. R. , Yang Chongyin
Published: August 2023

With the growing number of academic researchers focused on lithium-ion batteries, the cell format of choice is often overlooked. Coin cells that utilize either a lithium metal or greatly oversized graphite negative electrode are common but can provide unrealistic testing results when compared to commercial pouch-type cells. Instead, single-layer pouch cells provide a more similar format to those used in industry while not requiring large amounts of active material. Moreover, their assembly process allows for better positive/negative electrode alignment, allowing for assembly of single-layer pouch cells without negative electrode overhang. This work presents a comparison between coin, single-layer pouch, and stacked pouch cells, and shows that single-layer pouch cells without overhang perform best. As well, an Ultra-High Precision Coulometry experiment is performed to show that excess negative or positive electrode coating is detrimental to cell performance. Finally, a guide to assembling reproducible single-layer pouch cells without overhang is presented.
Inducible nitric oxide synthase activity mediates TNF-α-induced endothelial cell dysfunction
Authors: Liu Chen , Lei Sujuan , Cai Tianying , Cheng Yonglang , Bai Junjie , Fu Wenguang , Huang Meizhou
Published: August 2023

Inducible nitric oxide synthase (iNOS) deficiency during vascular endothelial dysfunction may affect the expression of tumor necrosis factor receptor-2 and vascular adhesion factors via cellular metabolic changes, thereby attenuating vascular endothelial dysfunction.
Exploring shared genetics between maximal oxygen uptake and disease: the HUNT study
Authors: Nordeidet Ada N. , Klevjer Marie , Wisløff Ulrik , Langaas Mette , Bye Anja
Published: August 2023

To our knowledge, this is the first genetic association study exploring how genes linked to cardiorespiratory fitness (CRF) relate to disease risk. By investigating shared genetics, we found indications that genetic variants linked to directly measured CRF also affect the level of blood creatinine, risk of diabetes, and endocarditis. Less certain findings showed that genetic variants of high CRF might cause lower body mass index, healthier HDL cholesterol, and lower resting heart rate.
Establishment of a prognostic model toward lung squamous cell carcinoma based on m7G-related genes in the cancer genome atlas
Authors: Wang Yongheng , Liu Yimin , Wang Rui , Cao Fuyuan , Guan Yi , Chen Yulu , An Binbin , Qin Sisi , Yao Sanqiao
Published: August 2023

Based on the differential expression of 7-methylguanosine (m7G) modification-associated genes between normal and lung squamous cell carcinoma (LUSC) tissues, and considering the performance of our m7G-related gene risk profiles as independent risk factors in predicting overall survival, we conclude that m7G modification is closely linked to the development of LUSC. In addition, this study offers a new genetic marker for predicting the prognosis of patients with LUSC and presents a crucial theoretical foundation for future investigations on the relationship between m7G modification-related genes, immunity, and drug sensitivity in LUSC.
Adoption of Biosecurity Practices in Smallholder Dairy Farms in Ethiopia
Authors: Nyokabi Ndungu S. , Berg Stefan , Mihret Adane , Almaw Gizat , Worku Gizachew Gemechu , Lindahl Johanna F. , Wood James L. N. , Moore Henrietta L.
Published: August 2023

Dairy production is an important livelihood source for smallholder dairy farmers who produce the majority of milk consumed and traded in Ethiopia. Dairy production is, however, constrained by livestock diseases that impact farm productivity, food safety, and animal welfare. Biosecurity measures (BSM) include all risk reduction strategies designed to avoid the introduction of pathogenic infections from outside and minimise the spread of diseases within dairy herds. This study used a cross-sectional survey to investigate the adoption of BSM in dairy farms in Addis Ababa and Oromia regions of Ethiopia. Using a questionnaire, scores for adopted external and internal BSM were calculated based on the Ghent’s University Biocheck tool to compare the performance of different farms in Ethiopia. The weighted external biosecurity score was 49.1%, which was below average (below 50% adoption), while the weighted internal biosecurity score was 55.5%. Low adoption of crucial BSM increases the risk of disease introduction into dairy farms and transmission within herds. Adoption of BSM at the farm level was driven by individual, demographic, and socio-economic drivers, including education, farming system, milk value chain, and farming experience among others. Results of this research reveal low adoption of BSM and the imperative to encourage farmers to implement BSM can lead to a reduction in disease pressures and, thus, a reduction in antibiotic use and increased dairy farms productivity, and improved animal health and welfare. Farmers can be encouraged through proactive engagement with veterinarians and extension professionals. Moreover, creating a favourable policy environment can support farmers to adopt and implement BSM, given the known fact that “prevention is better and cheaper than curing diseases.”
Predictors of Left Ventricular Mass Index One Year after Bioprosthetic Aortic Valve Replacement for Aortic Stenosis
Authors: Yamazaki Sachiko , Okawa Kazunari , Shunto Keisuke , Oka Katsuhiko , Ikemoto Koki , Takahashi Akiyuki
Published: August 2023

Background and Aim of Study.To evaluate predictors of residual left ventricular hypertrophy (LVH) one year after surgical aortic valve replacement (SAVR) in patients with aortic stenosis and clarify the relationship between long-term outcomes and predictors. Methods. We retrospectively reviewed 141 patients who underwent SAVR with a bioprosthetic valve. Left ventricular dimensions and mass index were assessed using serial transthoracic echocardiography. The difference in time course and the pattern of left ventricular mass index (LVMI) regression between patients with and without residual LVH one year after surgery were evaluated. The factors associated with LVMI one year after SAVR and the prognostic impact of these predictors on long-term outcomes were analyzed. Results. Although LVMI one year after surgery showed a significant decrease in patients with and without LVH, greater preoperative LVMI and lesser extent of LVMI decrease resulted in high residual LVMI at one year after SAVR in patients with LVH. The preoperative left ventricular end-diastolic dimension index (

p
=
0.027

) and preoperative left atrial dimension (

p
=
0.001

) were significant determinants of LVMI at one year after SAVR. A cut-off value of 30 mm/m2 or greater for the left ventricular end-diastolic dimension index was optimal for predicting high LVMI one year after SAVR. Overall survival was significantly lower with a left ventricular end-diastolic dimension index ≥30 mm/m2 (

p
=
0.017

, Log rank). Conclusions. High preoperative left ventricular end-diastolic dimension index and large left atrial dimension were associated with high LVMI one year after surgical aortic valve replacement. Preoperative left ventricular end-diastolic dimension index of >30 mm/m2 could predict adverse outcomes after surgical aortic valve replacement.
Extraction, Characterization, and Evaluation of Lepidium sativum Linn. Mucilage as a Mucoadhesive Polymer
Authors: Embafrash Berhe Hailemichael , Tesfay Mezgebo Desta , Abrha Solomon , Gebremeskel Haile Tsadkan , Molla Fantahun
Published: August 2023

Being biocompatible, less toxic, cheap, easily available, and environmentally friendly, there is an increased trust in natural polymers in the drug delivery system. Mucilages, among the natural polymers, are the primary metabolites of plants that have been widely utilized in pharmaceutical manufacturing for different purposes, and mucoadhesive is one among them. The present study was designed to investigate the use of LSM as a mucoadhesive polymer using ibuprofen as a model drug. The mucilage was extracted following an aqueous extraction method and its percentage yield was found to be 13.2% w/w. Besides, three microsphere formulations of ibuprofen were prepared using synthetic polymer hydroxyl propyl methyl cellulose (HPMC) K100M and the LSM in polymer to drug ratios of 1 : 1, 1 : 5, and 3 : 5 by applying ionotropic gelation followed by solvent evaporation methods. The microspheres were evaluated for various micromeritic properties and all the formulations exhibited free-flowing properties. Optical microscopic pictures of almost all the microspheres except F3 and F6 (which had more or less spherical shapes) were found to have irregular and discrete shapes. Besides, the surfaces of all the formulations were rough in texture. The drug entrapment efficiency of the microspheres was found to be between 52.08% ± 0.80 and 87.97% ± 0.72. The in-vitrowash-off test evidenced that almost 50 percent (especially F3) of the microspheres were able to adhere up to 18 h and showed remarkable bioadhesion properties. The in-vitro drug release profile indicated that all the formulations were able to prolong their drug release up to 12 h with a non-fickian release mechanism, except for F4, which followed a fickian release. Therefore, based on the findings of this study, LSM can be used as a potential alternative mucoadhesive excipient for sustained release formulations.
US Veterans Show Improvements in Subjective but Not Objective Sleep following Treatment for Posttraumatic Stress Disorder: Secondary Analyses from a Randomised Controlled Trial
Authors: Mathersul Danielle C. , Schulz-Heik R. Jay , Avery Timothy J. , Allende Santiago , Zeitzer Jamie M. , Bayley Peter J.
Published: August 2023

Background. Sleep disturbances are a prominent feature of posttraumatic stress disorder (PTSD), and poorer sleep quality is associated with higher PTSD severity. This highlights the importance of monitoring sleep outcomes alongside PTSD symptoms in treatments targeting PTSD. Yet few studies monitor both sleep and PTSD outcomes, unless sleep is the primary treatment target. Furthermore, inconsistencies remain about the effects of first-line, evidence-based PTSD treatments on sleep. Methods. Here, we explored changes in sleep in secondary analyses from a randomised controlled trial that originally assessed the noninferiority of a breathing-based yoga practice (Sudarshan kriya yoga; SKY) to a first-line PTSD treatment (cognitive processing therapy (CPT)) for clinically significant PTSD symptoms among US veterans (intent-to-treat

N
=
85

; per protocol

N
=
59

). Sleep was assessed via subjective (self-reported sleep diary), PTSD symptom severity items (self-reported and clinician-administered insomnia/nightmare sleep items), and objective (wrist actigraphy) measures. Results. Following treatment, subjective sleep diary measures of quality, latency, and wake duration showed small effect size (

d
=
.24
−
.39

) improvements, with no significant differences between treatment groups. Significant improvements were also observed in PTSD sleep symptoms, though CPT (

d
=
.34

) more reliably reduced nightmares while SKY (d = .44-.45) more reliably reduced insomnia. In contrast, there were no significant treatment-related effects for any of the actigraphy-measured sleep indices. Conclusions. To our knowledge, this is the first study to investigate sleep as an outcome of CPT or SKY for PTSD, across a combination of subjective diary, PTSD symptom severity, and objective actigraphic measures. Findings lend support to a growing body of evidence that trauma-focused psychotherapy for PTSD improves sleep and suggest that yoga-based interventions may also be beneficial for sleep among individuals with emotional or mental health disorders like PTSD. This trial is registered with NCT02366403.
Carbon-Neutralized Direct Methanol Fuel Cell Using Bifunctional (Methanol Oxidation/CO2 Reduction) Electrodes
Authors: Kim Yong Seok , Lee Jae Wook , Kim Byeongkyu , Bae Jong Wook , Chung Chan-Hwa
Published: August 2023

In this study, a carbon-neutralized direct methanol fuel cell (DMFC) using two bifunctional electrodes, Pd-Ag and Pt-Zn, has been designed. This system has two modes, which are fuel-cell mode and spontaneous CO2 reduction mode. In the operation of fuel-cell mode, the methanol has been oxidized into CO2 on the Pd-Ag electrode and generates electricity. In the next step of the operation, CO2, which is the product of fuel-cell mode, has been spontaneously reduced to CO on Pd-Ag, and electricity has been obtained. In contrast, the Pt in the Pt-Zn electrode catalyzes the oxygen reduction reaction in the fuel-cell mode, and the Zn in Pt-Zn is oxidized sacrificially in the CO2 reduction mode. During operation in the fuel-cell mode, a power density of 12.11 mW/cm2 has been obtained with the production of CO2. On the other hand, the power density out of the CO2 reduction mode has been 11.76 mW/cm2. In each mode, the faradaic efficiencies of CO2 and CO have been 98.81% and 89.11%, respectively.
Energy and Exergy Assessments of a Diesel-, Biodiesel-, and Ammonia-Fueled Compression Ignition Engine
Authors: Proniewicz Mateusz , Petela Karolina , Szlęk Andrzej , Przybyła Grzegorz , Nadimi Ebrahim , Ziółkowski Łukasz , Løvås Terese , Adamczyk Wojciech
Published: August 2023

The research is aimed at investigating ammonia in a compression ignition internal combustion engine as a promising alternative fuel towards decarbonization. This study presents energy and exergy assessments of a low-power engine for three cases of fuel supply, diesel oil, biodiesel oil, and ammonia with pilot biodiesel oil, across the entire engine’s range. While diesel or biodiesel was administered directly into the engine cylinder, the ammonia was delivered through port injection. The results show that the maximum thermal efficiency of 33.56% and exergy efficiency of 31.88% were found at 1800 rpm and 71% load for the diesel fuel system. For the biodiesel fuel system, the efficiencies were 32.72% and 30.93%, respectively, at 1800 rpm and 100% load, and for the ammonia with pilot biodiesel system, they were at 31.98% and 30.04%, respectively, for the same rpm and load. The exergy assessment indicates that exergy destruction, which accounts for the irreversibility of processes such as combustion and friction, is responsible for the greatest loss of useful energy. Optimizing these processes could significantly improve the engine’s performance for all three fuel cases. This research found that ammonia could successfully substitute diesel or biodiesel fuel, as the engine’s efficiency was comparable in all three tested scenarios; however, further research and optimization in terms of the ammonia-fueled engine emissions are required.
Parkin Deficiency Suppresses Antigen Presentation to Promote Tumor Immune Evasion and Immunotherapy Resistance
Authors: Perales-Linares Renzo , Leli Nektaria Maria , Mohei Hesham , Beghi Silvia , Rivera Osvaldo D. , Kostopoulos Nektarios , Giglio Andrea , George Subin S. , Uribe-Herranz Mireia , Costabile Francesca , Pierini Stefano , Pustylnikov Sergei , Skoufos Giorgos , Barash Yoseph , Hatzigeorgiou Artemis G. , Koumenis Constantinos , Maity Amit , Lotze Michael T. , Facciabene Andrea
Published: August 2023

Parkin is an E3 ubiquitin ligase, which plays a key role in the development of Parkinson disease. Parkin defects also occur in numerous cancers, and a growing body of evidence indicates that Parkin functions as a tumor suppressor that impedes a number of cellular processes involved in tumorigenesis. Here, we generated murine and human models that closely mimic the advanced-stage tumors where Parkin deficiencies are found to provide deeper insights into the tumor suppressive functions of Parkin. Loss of Parkin expression led to aggressive tumor growth, which was associated with poor tumor antigen presentation and limited antitumor CD8+ T-cell infiltration and activation. The effect of Parkin deficiency on tumor growth was lost following depletion of CD8+ T cells. In line with previous findings, Parkin deficiency was linked with mitochondria-associated metabolic stress, PTEN degradation, and enhanced Akt activation. Increased Akt signaling led to dysregulation of antigen presentation, and treatment with the Akt inhibitor MK2206-2HCl restored antigen presentation in Parkin-deficient tumors. Analysis of data from patients with clear cell renal cell carcinoma indicated that Parkin expression was downregulated in tumors and that low expression correlated with reduced overall survival. Furthermore, low Parkin expression correlated with reduced patient response to immunotherapy. Overall, these results identify a role for Parkin deficiency in promoting tumor immune evasion that may explain the poor prognosis associated with loss of Parkin across multiple types of cancer.

Significance:

Parkin prevents immune evasion by regulating tumor antigen processing and presentation through the PTEN/Akt network, which has important implications for immunotherapy treatments in patients with Parkin-deficient tumors.
Supplementary Table S3 from Integrated Multimodal Analyses of DNA Damage Response and Immune Markers as Predictors of Response in Metastatic Triple-Negative Breast Cancer in the TNT Trial (NCT00532727)
Authors: Tovey Holly , Sipos Orsolya , Parker Joel S. , Hoadley Katherine A. , Quist Jelmar , Kernaghan Sarah , Kilburn Lucy , Salgado Roberto , Loi Sherene , Kennedy Richard D. , Roxanis Ioannis , Gazinska Patrycja , Pinder Sarah E. , Bliss Judith , Perou Charles M. , Haider Syed , Grigoriadis Anita , Tutt Andrew , Cheang Maggie Chon U
Published: August 2023

Supplemetary table 3 - Change in signature scores from primary to recurrence
Supplementary Table S2 from Integrated Multimodal Analyses of DNA Damage Response and Immune Markers as Predictors of Response in Metastatic Triple-Negative Breast Cancer in the TNT Trial (NCT00532727)
Authors: Tovey Holly , Sipos Orsolya , Parker Joel S. , Hoadley Katherine A. , Quist Jelmar , Kernaghan Sarah , Kilburn Lucy , Salgado Roberto , Loi Sherene , Kennedy Richard D. , Roxanis Ioannis , Gazinska Patrycja , Pinder Sarah E. , Bliss Judith , Perou Charles M. , Haider Syed , Grigoriadis Anita , Tutt Andrew , Cheang Maggie Chon U
Published: August 2023

Supplementary table 2 - Model estimates for logistic regression models of objective response rate and linear regression models of restricted PFS by treatment group
Supplementary Figure S5 from Integrated Multimodal Analyses of DNA Damage Response and Immune Markers as Predictors of Response in Metastatic Triple-Negative Breast Cancer in the TNT Trial (NCT00532727)
Authors: Tovey Holly , Sipos Orsolya , Parker Joel S. , Hoadley Katherine A. , Quist Jelmar , Kernaghan Sarah , Kilburn Lucy , Salgado Roberto , Loi Sherene , Kennedy Richard D. , Roxanis Ioannis , Gazinska Patrycja , Pinder Sarah E. , Bliss Judith , Perou Charles M. , Haider Syed , Grigoriadis Anita , Tutt Andrew , Cheang Maggie Chon U
Published: August 2023

Heatmap showing clustering of all module scores filtered for a significant interaction with treatment. Our original clusters are shown against the new clusters at the top of the heatmap.
Supplementary Figure S1 from Integrated Multimodal Analyses of DNA Damage Response and Immune Markers as Predictors of Response in Metastatic Triple-Negative Breast Cancer in the TNT Trial (NCT00532727)
Authors: Tovey Holly , Sipos Orsolya , Parker Joel S. , Hoadley Katherine A. , Quist Jelmar , Kernaghan Sarah , Kilburn Lucy , Salgado Roberto , Loi Sherene , Kennedy Richard D. , Roxanis Ioannis , Gazinska Patrycja , Pinder Sarah E. , Bliss Judith , Perou Charles M. , Haider Syed , Grigoriadis Anita , Tutt Andrew , Cheang Maggie Chon U
Published: August 2023

Additional associations between signatures.
A. Distribution of CIN70 by HRD score and genomic scars (NtAI tertiles, AiCna tertiles and HLAMP). B Distribution of RPS by HRD score and genomic scars (NtAI tertiles, AiCna tertiles and HLAMP). C. Distribution of PARPi7 by HRD score and genomic scars (NtAI tertiles, AiCna tertiles and HLAMP). D. Distribution of TILs by HRD score, E. Distribution of ConcensusTME average score by HRD score F. ConsensusTME cell type estimates are highly correlated excluding fibroblasts.Correlation assessed using Spearman correlation. HRD<42 = HRD low; HRD≥42 = HRD high.
Data from Resistance to selective FGFR inhibitors in FGFR-driven urothelial cancer
Authors: Facchinetti Francesco , Hollebecque Antoine , Braye Floriane , Vasseur Damien , Pradat Yoann , Bahleda Rastislav , Pobel Cedric , Bigot Ludovic , Deas Olivier , Florez Arango Juan David , Guaitoli Giorgia , Mizuta Hayato , Combarel David , Tselikas Lambros , Michiels Stefan , Nikolaev Sergey I. , Scoazec Jean-Yves , Ponce-Aix Santiago , Besse Benjamin , Olaussen Ken A. , Loriot Yohann , Friboulet Luc
Published: August 2023

Abstract
Several FGFR inhibitors are approved or in clinical development for the treatment of FGFR-driven urothelial cancer, and molecular mechanisms of resistance leading to patient relapses have not been fully explored. We identified 21 FGFR-driven urothelial cancer patients treated with selective FGFR inhibitors and analyzed post-progression tissue and/or circulating tumor DNA (ctDNA). We detected single mutations in the FGFR tyrosine kinase domain in seven (33%) patients (FGFR3 N540K, V553L/M, V555L/M, E587Q; FGFR2 L551F) and multiple mutations in one (5%) case (FGFR3 N540K, V555L, L608V). Using Ba/F3 cells, we defined their spectrum of resistance/sensitivity to multiple selective FGFR inhibitors. Eleven (52%) patients harbored alterations in the PI3K-mTOR pathway (n = 5 TSC1/2, n = 5 PIK3CA, n = 1 NF2, n = 1 PTEN). In patient-derived models, erdafitinib was synergic with pictilisib in the presence of PIK3CA E545K, while erdafitinib-gefitinib combination was able to overcome bypass resistance mediated by EGFR activation.
Data from Resistance to Selective FGFR Inhibitors in FGFR-Driven Urothelial Cancer
Authors: Facchinetti Francesco , Hollebecque Antoine , Braye Floriane , Vasseur Damien , Pradat Yoann , Bahleda Rastislav , Pobel Cédric , Bigot Ludovic , Déas Olivier , Florez Arango Juan David , Guaitoli Giorgia , Mizuta Hayato , Combarel David , Tselikas Lambros , Michiels Stefan , Nikolaev Sergey I. , Scoazec Jean-Yves , Ponce-Aix Santiago , Besse Benjamin , Olaussen Ken A. , Loriot Yohann , Friboulet Luc
Published: August 2023

Abstract
Several fibroblast growth factor receptor (FGFR) inhibitors are approved or in clinical development for the treatment of FGFR-driven urothelial cancer, and molecular mechanisms of resistance leading to patient relapses have not been fully explored. We identified 21 patients with FGFR-driven urothelial cancer treated with selective FGFR inhibitors and analyzed postprogression tissue and/or circulating tumor DNA (ctDNA). We detected single mutations in the FGFR tyrosine kinase domain in seven (33%) patients (FGFR3 N540K, V553L/M, V555L/M, E587Q; FGFR2 L551F) and multiple mutations in one (5%) case (FGFR3 N540K, V555L, and L608V). Using Ba/F3 cells, we defined their spectrum of resistance/sensitivity to multiple selective FGFR inhibitors. Eleven (52%) patients harbored alterations in the PI3K–mTOR pathway (n = 4 TSC1/2, n = 4 PIK3CA, n = 1 TSC1 and PIK3CA, n = 1 NF2, n = 1 PTEN). In patient-derived models, erdafitinib was synergistic with pictilisib in the presence of PIK3CA E545K, whereas erdafitinib–gefitinib combination was able to overcome bypass resistance mediated by EGFR activation.
Significance:
In the largest study on the topic thus far, we detected a high frequency of FGFR kinase domain mutations responsible for resistance to FGFR inhibitors in urothelial cancer. Off-target resistance mechanisms involved primarily the PI3K–mTOR pathway. Our findings provide preclinical evidence sustaining combinatorial treatment strategies to overcome bypass resistance.
Table S8 from Single-cell profiling uncovers a Muc4-expressing metaplastic gastric cell type sustained by Helicobacter pylori-driven inflammation
Authors: O'Brien Valerie Phoebe , Kang Yuqi , Shenoy Meera K , Finak Greg , Young William C , Dubrulle Julien , Koch Lisa , Rodriguez Martinez Armando E , Williams Jeffery , Donato Elizabeth , Batra Surinder K. , Yeung Cecilia C.S. , Grady William M , Koch Meghan A , Gottardo Raphael , Salama Nina R.
Published: August 2023

List of antibodies, lectins and probes used in this study.
Table S5 from Single-cell profiling uncovers a Muc4-expressing metaplastic gastric cell type sustained by Helicobacter pylori-driven inflammation
Authors: O'Brien Valerie Phoebe , Kang Yuqi , Shenoy Meera K , Finak Greg , Young William C , Dubrulle Julien , Koch Lisa , Rodriguez Martinez Armando E , Williams Jeffery , Donato Elizabeth , Batra Surinder K. , Yeung Cecilia C.S. , Grady William M , Koch Meghan A , Gottardo Raphael , Salama Nina R.
Published: August 2023

Pathway analysis of metaplastic pit cells (pit_2, pit_6 and pit_8) versus classical pit cells (pit_1, pit_3, pit_4, pit_5 and pit_7) from UMAP #2.
Table S4 from Single-cell profiling uncovers a Muc4-expressing metaplastic gastric cell type sustained by Helicobacter pylori-driven inflammation
Authors: O'Brien Valerie Phoebe , Kang Yuqi , Shenoy Meera K , Finak Greg , Young William C , Dubrulle Julien , Koch Lisa , Rodriguez Martinez Armando E , Williams Jeffery , Donato Elizabeth , Batra Surinder K. , Yeung Cecilia C.S. , Grady William M , Koch Meghan A , Gottardo Raphael , Salama Nina R.
Published: August 2023

The 10 most differentially expressed genes within each cluster of UMAP #2.
Supplementary Methods from Single-cell profiling uncovers a Muc4-expressing metaplastic gastric cell type sustained by Helicobacter pylori-driven inflammation
Authors: O'Brien Valerie Phoebe , Kang Yuqi , Shenoy Meera K , Finak Greg , Young William C , Dubrulle Julien , Koch Lisa , Rodriguez Martinez Armando E , Williams Jeffery , Donato Elizabeth , Batra Surinder K. , Yeung Cecilia C.S. , Grady William M , Koch Meghan A , Gottardo Raphael , Salama Nina R.
Published: August 2023

Expanded Materials and Methods.
Figure S4 from Single-cell profiling uncovers a Muc4-expressing metaplastic gastric cell type sustained by Helicobacter pylori-driven inflammation
Authors: O'Brien Valerie Phoebe , Kang Yuqi , Shenoy Meera K , Finak Greg , Young William C , Dubrulle Julien , Koch Lisa , Rodriguez Martinez Armando E , Williams Jeffery , Donato Elizabeth , Batra Surinder K. , Yeung Cecilia C.S. , Grady William M , Koch Meghan A , Gottardo Raphael , Salama Nina R.
Published: August 2023

The epithelial subclusters pit_2, pit_6, pit_8 and neck_2 are expanded in Hp+KRAS+ mice.
Figure S11 from Single-cell profiling uncovers a Muc4-expressing metaplastic gastric cell type sustained by Helicobacter pylori-driven inflammation
Authors: O'Brien Valerie Phoebe , Kang Yuqi , Shenoy Meera K , Finak Greg , Young William C , Dubrulle Julien , Koch Lisa , Rodriguez Martinez Armando E , Williams Jeffery , Donato Elizabeth , Batra Surinder K. , Yeung Cecilia C.S. , Grady William M , Koch Meghan A , Gottardo Raphael , Salama Nina R.
Published: August 2023

Gastric inflammation is greatest in Hp+KRAS+ mice.
TABLE 2 from Cancer Epidemiology in the Northeastern United States (2013–2017)
Authors: Rees Judy R. , Weiss Julie E. , Gunn Christine M. , Carlos Heather A. , Dragnev Nathalie C. , Supattapone Emma Y. , Tosteson Anna N.A. , Kraft Sally A. , Vahdat Linda T. , Peacock Janet L.
Published: August 2023

Five-year age-standardized relative survival ratios for cancers diagnosed 2012–2018, follow-up through 2019 by registry, all races, for ME, NH, VT with the United States
Supplementary Table S3 from Cancer Epidemiology in the Northeastern United States (2013–2017)
Authors: Rees Judy R. , Weiss Julie E. , Gunn Christine M. , Carlos Heather A. , Dragnev Nathalie C. , Supattapone Emma Y. , Tosteson Anna N.A. , Kraft Sally A. , Vahdat Linda T. , Peacock Janet L.
Published: August 2023

Patterns of incidence and mortality in Northern New England compared to the United States, among non-Hispanic White adults
Supplementary Table S2 from Cancer Epidemiology in the Northeastern United States (2013–2017)
Authors: Rees Judy R. , Weiss Julie E. , Gunn Christine M. , Carlos Heather A. , Dragnev Nathalie C. , Supattapone Emma Y. , Tosteson Anna N.A. , Kraft Sally A. , Vahdat Linda T. , Peacock Janet L.
Published: August 2023

Comparison of Age-standardized Incidence of Leading Cancer Sites, All Races, for Maine, New Hampshire, Vermont with United States 2013–2017
FIGURE 5 from Cancer Epidemiology in the Northeastern United States (2013–2017)
Authors: Rees Judy R. , Weiss Julie E. , Gunn Christine M. , Carlos Heather A. , Dragnev Nathalie C. , Supattapone Emma Y. , Tosteson Anna N.A. , Kraft Sally A. , Vahdat Linda T. , Peacock Janet L.
Published: August 2023

Leading cancer sites ratio of age-standardized mortality rates (RR) and 99% CIs† for combined ME, NH, VT versus the United States, overall and among non-Hispanic White 2013–2017.
BRCA2 Frameshift Mutation in de novo Small-Cell Neuroendocrine Carcinoma of the Prostate: A Case Report
Authors: Okubo Keisuke , Narita Shintaro , Koizumi Atsushi , Takahashi Yoshiko , Sagehashi Ryuichiro , Mori Kanami , Sobu Ryuta , Sato Hiromi , Kashima Soki , Kobayashi Mizuki , Yamamoto Ryohei , Nara Taketoshi , Numakura Kazuyuki , Saito Mitsuru , Nanjyo Hiroshi , Habuchi Tomonori
Published: August 2023

A 66-year-old male was diagnosed with cT4N0M1b small-cell neuroendocrine carcinoma of the prostate. Four months after the administration of combined androgen blockade, multiple novel metastatic regions in the lung and liver and progression of bone metastasis were observed. The patient was referred to our hospital because of biochemical and radiographic progression after four cycles of docetaxel as a first-line therapy for castration-resistant prostate cancer. Transurethral resection of the prostate and hepatic biopsy revealed small-cell carcinoma with positive expression of neuroendocrine markers. The FoundationOne CDx next-generation sequencing test revealed several pathogenic variants, including BRCA2 (W1692fs*3), KEAP1 (R320W), and TP53 (C2385) mutation. After four cycles of chemotherapy with carboplatin plus etoposide (CE), the metastatic regions regressed markedly. The prostate-specific antigen (PSA) and neuron-specific enolase (NSE) level decreased by 96.9% and 91.6%, respectively. However, 2 months after the completion of four cycles of CE, elevation of tumor marker levels, and re-growth of the metastatic regions were observed. Although olaparib, a poly (ADP-ribose) polymerase inhibitor (PARPi), achieved a 45.2% decrease in NSE, the patient rejected to continue therapy because of G2 adverse events. After receiving an additional two cycles of CE and one cycle of cabazitaxel, the patient died because of cancer progression 24 months after the initial treatment for prostate cancer. Here, we present a case of BRCA2-altered small-cell neuroendocrine prostate cancer treated with both platinum-containing chemotherapy and PARPi. Both therapies achieved an initial response; however, durable responses were not obtained. Additional discussion regarding the optimal treatment strategy for BRCA-altered small-cell/neuroendocrine prostate cancer is required.
Impact of Hemodialysis on Left Ventricular-Arterial Coupling in End-Stage Renal Disease Patients
Authors: Zuo Ming-Liang , Chen Qiu-Yi , Pu Lei , Shi Lan , Wu Dan , Li Hua , Luo Xian , Yin Li-Xue , Siu Chung-Wah , Hong Da-Qing , Gan Hua
Published: August 2023

Introduction: As a key determinant of cardiovascular performance, vascular-arterial coupling (VAC) has been reported to be a predictor of clinical outcomes in various clinical scenarios. However, few studies have explored how acute fluid removal during hemodialysis (HD) impacts the interaction between cardiac function and the arterial system. Methods: We recruited 317 HD patients from an established renal dialysis unit for this cross-sectional study and a total of 285 were included in the final analyses. We measured left ventricle end-systolic elastance (Ees), the effective arterial elastance (Ea), and VAC before and after HD using noninvasive echocardiographic measurements. We also compared echocardiographic and hemodynamic parameters in ventriculo-arterial coupling and ventriculo-arterial uncoupling patients. Results: HD significantly altered partial ventricular and vascular function parameters such as blood pressure, left ventricular end-diastolic volume, stroke volume, left ventricular ejection fraction, and systemic vascular resistance index. Ea increased following HD from 3.5 ± 1.4 to 4.2 ± 1.8 mm Hg/mL (p < 0.0001), Ees increased following HD from 7.9 ± 5.5 to 9.2 ± 6.9 mm Hg/mL (p = 0.04), whereas VAC did not markedly alter as a result of HD. Ventriculo-arterial uncoupling was found to be related to abnormal cardiac structure and worse systolic function. Conclusions: VAC obtained from echocardiography is likely to be load-independent and useful as a reliable index for stratifying the risk of cardiovascular diseases in HD patients. Further investigations on larger patient cohorts are needed to further validate our findings.
Prodromal Dementia with Lewy Bodies: A Case Series of the 3 Prodromal Types from Clinical Practice
Authors: Tholanikunnel Tracy , Chapin Benjamin , Armstrong Melissa
Published: August 2023

Prodromal dementia with Lewy bodies (DLB) refers to a state prior to the onset of dementia with clinical signs or symptoms that may indicate the future development of DLB. Prodromal symptoms can include not only cognitive deficits but also a mix of clinical features including sleep disorders, autonomic dysfunction, and neuro-psychiatric disturbances. While diagnostic criteria for the subtypes of prodromal DLB were recently published, they are largely used in research settings. However, these criteria have important implications for clinical practice. Recognition of prodromal DLB stages can lead to identifying deficits sooner, improved patient and family counseling, and advance care planning. This case series presents examples of the 3 subtypes of prodromal DLB – mild cognitive impairment onset, delirium onset, and psychiatric onset – to help clinicians identify individuals who may be on a trajectory to develop DLB.
O oceano
Authors:   Universidade de São Paulo. Instituto Oceanográfico, Todam Agatha , Llonch Brenda Gulfier Sanchez ,   Universidade de São Paulo. Instituto Oceanográfico, Silva Giovanna Reis ,   Universidade de São Paulo. Instituto Oceanográfico, Panho João Pedro Alonso ,   Universidade de São Paulo. Instituto Oceanográfico, Brito Maithê Kapor de ,   Universidade de São Paulo. Instituto Oceanográfico, Moreira Priscila Saviolo ,   Universidade de São Paulo. Instituto Oceanográfico, Signori Camila Negrão ,   Universidade de São Paulo. Instituto Oceanográfico
Published: August 2023

Este livro é um produto do Projeto EAMar, produzido no ano de 2021 por alunos de graduação do curso de Bacharelado em Oceanografia, do Instituto Oceanográfico da Universidade de São Paulo e tem por finalidade oferecer material didático como suporte para professores e alunos participantes do projeto. É o primeiro livro da série "Educação Ambiental Marinha" e o conteúdo, aqui presente, traz uma introdução ao estudo do oceano e à ciência oceanográfica, tendo como principal objetivo, disseminar os princípios da cultura oceânica, contribuindo, dessa forma, para um olhar multidimensional para a Década do Oceano. O ano de 2021 marca o início da Década das Nações Unidas da Ciência Oceânica para o Desenvolvimento Sustentável (doravante Década do Oceano), liderada pela Comissão Oceanográfica Intergovernamental da UNESCO (COI-UNESCO) e segue até 2030. Sem dúvida, é um momento singular de difusão da Oceanografia, de desenvolvimento da Cultura Oceânica entre jovens estudantes e de articulação entre todos os setores da sociedade em prol de uma relação mais sustentável com o oceano. Também faz parte deste trabalho o projeto "Ciência Cidadã" que, através do engajamento de professores e alunos, tem por objetivo, utilizando método científico, estudar a erosão de praias, através do monitoramento fotográfico da linha de costa.
IO-USPeople na fronteira da vida no oceano: A Ciência que fazemos para o oceano que queremos
Authors:   Universidade de São Paulo. Instituto Oceanográfico, Signori Camila Negrão , Silva Anna Thereza S. A. ,   Universidade de São Paulo. Instituto Oceanográfico
Published: August 2023

O livro eletrônico IO-USPeople na fronteira da vida no oceano: A Ciência que fazemos para o oceano que queremos é uma iniciativa do Instituto Oceanográfico da Universidade de São Paulo (IO-USP) para atender ao objetivo 14 da Agenda 2030 da ONU sobre a “Vida na Água: Conservação e uso sustentável dos oceanos, dos mares e dos recursos marinhos para o desenvolvimento sustentável”. Dentre as ações a serem alcançadas, constam a necessidade de aumentar o conhecimento científico, a redução da poluição, a gestão sustentável e a ampliação de áreas de proteção marinha, o enfrentamento e a minimização dos impactos da acidificação do oceano, a regulação da pesca e a promoção das ciências marinhas. O objetivo 14 se destacou dentre os objetivos constituintes de um pacto para um mundo melhor, por sua forte interconexão e indivisibilidade dos demais objetivos, o que resultou na escolha da ONU pela Década da Ciência Oceânica para o Desenvolvimento Sustentável (2021-2030). Há inúmeras motivações para a busca pelo conhecimento sobre o oceano, como por exemplo, por ser essencial à vida e cobrir três quartos do Planeta Terra; conectar as populações e mercados; representar nossas heranças natural e cultural; fornecer mais da metade do oxigênio que respiramos; absorver mais de um quarto do dióxido de carbono; exercer papel vital no ciclo da água e no sistema climático; ser fonte importante de biodiversidade e serviços ecossistêmicos; contribuir para o desenvolvimento sustentável, bem como para a erradicação da pobreza, segurança alimentar e nutrição, comércio e transporte marítimo, trabalho digno e fonte de renda. No IO-USP, atividades científicas, educacionais e de extensão já vem sendo desenvolvidas para atingir as sete metas propostas pela Década do Oceano (oceano limpo, saudável e resiliente, previsível, seguro, produtivo e explorado sustentavelmente, transparente, conhecido e valorizado por todos) e que contribuem indubitavelmente para o avanço do conhecimento sobre o oceano no contexto regional, nacional e internacional. Este livro tem como principal objetivo disseminar o conhecimento produzido dentro do IO-USP para informar, conscientizar, sensibilizar e engajar o público leigo sobre a Vida na Água, baseado nos avanços da Ciência Oceanográfica, auxiliando inclusive para tomadas de decisão.
Dynamics of High-Density Unipolar Epicardial Electrograms During PFA
Authors: Amorós-Figueras Gerard , Casabella-Ramon Sergi , Moreno-Weidmann Zoraida , Ivorra Antoni , Guerra Jose M. , García-Sánchez Tomás
Published: August 2023

BACKGROUND:

Pulsed field ablation (PFA) is a novel nonthermal cardiac ablation technology based on irreversible electroporation (IRE). While areas of IRE lead to durable lesions, the surrounding regions, where reversible electroporation occurs, recover. The behavior of local electrograms in areas of different electroporation levels remains unknown. The goal of this study is to characterize electrogram dynamics after PFA in IRE and reversible electroporation areas.

METHODS:

A total of 6 domestic swine were used. PFA was applied in the epicardium of the right and left ventricles using a focal monopolar catheter. Additional radiofrequency ablations were performed. Epicardial unipolar electrograms were acquired at baseline and for 60 minutes post PFA/radiofrequency ablation using a high-density electrode matrix attached to the epicardium. Electrogram dynamics were analyzed in areas corresponding to different levels of electroporation. Acute lesion formation was assessed after 3 to 5 hours by triphenyl tetrazolium chloride staining.

RESULTS:

Electrogram analysis demonstrated a clear association between electrogram changes and the level of electroporation. Immediately after PFA, electrograms displayed the following: a significant decrease in R/S-wave amplitude; a large elevation of the ST-segment; and a large decrease in their |(dV/dt)|
max
. Marked changes in electrograms were observed beyond the lesion area. Thereafter, a gradual recovery was observed. The evolution of all the electrogram parameters throughout the 60 minutes after PFA was significantly different (
P
<0.05) between the IRE and reversible electroporation areas. Acute lesion staining showed significantly larger depth for PFA lesions compared with radiofrequency ablation.

CONCLUSIONS:

This study shows that unipolar electrograms can differentiate between reversible electroporation and IRE areas during the first 30 minutes post ablation. Differences after the first 30 minutes are less evident. Our findings could result useful for immediate lesion assessment after PFA and warrant further investigation.
Changes in Doppler‐Derived Kidney Venous Flow and Adverse Cardiorenal Outcomes in Patients With Heart Failure
Authors: Husain‐Syed Faeq , Singam Narayana Sarma V. , Viehman Jason K. , Vaughan Lisa , Bauer Pascal , Gall Henning , Tello Khodr , Richter Manuel J. , Yogeswaran Athiththan , Romero‐González Gregorio , Rosner Mitchell H. , Ronco Claudio , Assmus Birgit , Ghofrani Hossein Ardeschir , Seeger Werner , Birk Horst‐Walter , Kashani Kianoush B.
Published: August 2023

Background

The impact of changes in Doppler‐derived kidney venous flow in heart failure (HF) is not well studied. We aimed to investigate the association of Doppler‐derived kidney venous stasis index (KVSI) and intrakidney venous‐flow (IKVF) patterns with adverse cardiorenal outcomes in patients with HF.

Methods and Results

In this observational cohort study, consecutive inpatients with HF referred to a nephrologist because of a history of diuretic resistance and abnormal kidney function (n=216) underwent spectral kidney assessments after admission (Doppler 1) and 25 to 35 days later (Doppler 2) to identify IKVF patterns (continuous/pulsatile/biphasic/monophasic) and KVSI levels. Cox proportional hazard regression models were used to evaluate the associations between KVSI/IKVF patterns at Doppler 1 as well as changes from Doppler 1 to Doppler 2 and risk of cardiorenal events up to 18 months after admission. Worsening HF or death occurred in 126 patients. Both baseline KVSI (hazard ratio [HR], 1.49 [95% CI, 1.37–1.61] per 0.1‐unit increase) and baseline IKVF pattern (HR, 2.47 [95% CI, 2.01–3.04] per 1 pattern severity increase) were significantly associated with worsening HF/death. Increases in both KVSI and IKVF pattern severity from Doppler 1 to 2 were also associated with an increased risk of worsening HF/death (HR, 3.00 [95% CI, 2.08–4.32] per 0.1‐unit increase change; and HR, 6.73 [95% CI, 3.27–13.86] per 1 pattern increase in severity change, respectively). Similar results were observed for kidney outcomes.

Conclusions

Baseline kidney venous flow predicted adverse cardiorenal events, and inclusion of serial kidney venous flow in cardiorenal risk stratification could facilitate clinical decision‐making for patients with HF.

Registration

URL:
https://www.clinicaltrials.gov
; Unique identifier: NCT03039959.
Subclinical Left Ventricular Dysfunction and Ventricular Arrhythmias in Older Adults With Normal Ejection Fraction
Authors: Yoshida Yuriko , Jin Zhezhen , Nakanishi Koki , Matsumoto Kenji , Homma Shunichi , Mannina Carlo , Shames Sofia , Elkind Mitchell S. V. , Rundek Tatjana , Di Tullio Marco R.
Published: August 2023

Background

Premature ventricular contractions (PVCs) and nonsustained ventricular tachycardia (NSVT) are known to be associated with reduced left ventricular (LV) ejection fraction and adverse outcomes in patients with structural heart disease. The relationship between subclinical LV dysfunction and ventricular arrhythmias in the general population is not established.

Methods and Results

Participants in the SAFARIS (Subclinical Atrial Fibrillation and Risk of Ischemic Stroke) study with normal left ventricular ejection fraction (n=503; mean age 77 years, 63% women) underwent 14‐day electrocardiographic monitoring and 2‐dimensional echocardiography. Frequent PVCs were defined as PVCs >500 per 24 hours and NSVT as ≥4 consecutive ventricular ectopic beats. Reduced LV global longitudinal strain (GLS) was used as an indicator of subclinical LV dysfunction. Seventy‐six participants (15.1%) had PVCs >500/d, 117 (23.3%) had NSVT episodes. LV GLS was significantly reduced in both frequent PVCs and NSVT groups (
P
<0.01). In multivariable analyses, lower LV GLS was associated with frequent PVCs (adjusted odds ratio [aOR], 1.19 [95% CI, 1.09–1.30 per unit reduction];
P
<0.001) and NSVT (aOR, 1.09 [95% CI, 1.01–1.17];
P
=0.036) independently of established risk factors and other echocardiographic parameters. Abnormal LV GLS (>−15.8%) carried a 2‐fold increase in risk of ventricular arrhythmias (aOR, 2.18,
P
=0.029 for PVCs; aOR, 2.09,
P
=0.026 for NSVT).

Conclusions

PVCs and NSVT episodes were frequent in this community‐based elderly cohort with normal left ventricular ejection fraction and were independently associated with lower LV GLS. The association between LV dysfunction and ventricular arrhythmias is present at an early, subclinical stage, an observation that carries possible preventative implications.
Continuous Versus Bolus Thermodilution‐Derived Coronary Flow Reserve and Microvascular Resistance Reserve and Their Association With Angina and Quality of Life in Patients With Angina and Nonobstructive Coronaries: A Head‐to‐Head Comparison
Authors: Jansen Tijn P. J. , de Vos Annemiek , Paradies Valeria , Dimitriu‐Leen Aukelien , Crooijmans Caïa , Elias‐Smale Suzette , Rodwell Laura , Maas Angela H. E. M. , Smits Pieter C. , Pijls Nico , van Royen Niels , Damman Peter
Published: August 2023

Background

Coronary flow reserve (CFR) and microvascular resistance reserve (MRR) are physiological parameters to assess coronary microvascular dysfunction. CFR and MRR can be assessed using bolus or continuous thermodilution, and the correlation between these methods has not been clarified. Furthermore, their association with angina and quality of life is unknown.

Methods and Results

In total, 246 consecutive patients with angina and nonobstructive coronary arteries from the multicenter Netherlands Registry of Invasive Coronary Vasomotor Function Testing (NL‐CFT) were investigated. The 36‐item Short Form Health Survey Quality of Life and Seattle Angina questionnaires were completed by 153 patients before the invasive measurements. CFR and MRR were measured consecutively with bolus and continuous thermodilution. Mean continuous thermodilution‐derived coronary flow reserve (CFR
abs
) was significantly lower than mean bolus thermodilution‐derived coronary flow reserve (CFR
bolus
) (2.6±1.0 versus 3.5±1.8;
P
<0.001), with a modest correlation (
ρ
=0.305;
P
<0.001). Mean continuous thermodilution‐derived microvascular resistance reserve (MRR
abs
) was also significantly lower than mean bolus thermodilution‐derived MRR (MRR
bolus
) (3.1±1.1 versus 4.2±2.5;
P
<0.001), with a weak correlation (
ρ
=0.280;
P
<0.001). CFR
bolus
and MRR
bolus
showed no correlation with any of the angina and quality of life domains, whereas CFR
abs
and MRR
abs
showed a significant correlation with physical limitation (
P
=0.005,
P
=0.009, respectively) and health (
P
=0.026,
P
=0.012). In a subanalysis in patients in whom spasm was excluded, the correlation further improved (MRR
abs
versus physical limitation:
ρ
=0.363;
P
=0.041, MRR
abs
versus physical health:
ρ
=0.482;
P
=0.004). No association with angina frequency and stability was found.

Conclusions

Absolute flow measurements using continuous thermodilution to calculate CFR
abs
and MRR
abs
weakly correlate with, and are lower than, the surrogates CFR
bolus
and MRR
bolus
. Absolute flow parameters showed a relationship with physical complaints. No relationship with angina frequency and stability was found.