bluuebunny/crossref_metadata_2025 huggingface dataset:
DETERMINAN PERATAAN LABA PADA PERUSAHAAN INDEKS LQ 45 DI BURSA EFEK INDONESIA PADA TAHUN 2016 – 2018
Authors: Fauziyanti Wachidah , Eryliana Esa Firda
Published: July 2023

Purpose of study this is analyze effect of return on assets, financial leverage, size company , net profit margin against alignment profit .

The analytical method used are descriptive statistics and logistic regression with previously already model feasibility test and assumption test were carried out classic . Data collection was carried out with documentation sourced secondary data _ from report LQ45 index company finance listed on the Indonesia Stock Exchange for the 2016-2018 period .

ROA research results have no negative effect significant , financial leverage has an effect positive , size company take effect positive and influential NPM positive significant to income smoothing . Advice for study for add sample and expand type company as well as enter a new variable that has effect to alignment profit
TINDAKAN PENGHINDARAN PAJAK PADA PERUSAHAAN MANUFAKTUR DI BURSA EFEK INDONESIA
Authors: HENDRAYANTI SILVIA
Published: July 2023

Pajak merupakan pungutan negara terhadap orang pribadi maupun badan yang sifatnya wajib dan memaksa yang akan dipergunakan oleh negara untuk kemakmuran rakyat tetapi tidak mendapat timbal balik secara langsung. tax avoidance merupakan pengaturan untuk meminimalkan atau menghilangkan beban pajak dengan mempertimbangkan akibat pajak yang ditimbulkannya, dan bukan sebagai pelanggaran pajak karena usaha wajib pajak untuk menguragi, menghindari, meminimumkan atau meringankan beban pajak dilakukan dengan cara yang di mungkinkan oleh undang-undang pajak. Penelitian ini bertujuan untuk mengetahui pengaruh komite audit, kualitas audit, dan pertumbuhan penjualan terhadap penghindatan pajak. Penelitian ini dilakukan pada perusahaan manufaktur yang terdaftar di Bursa Efek Indonesia periode 2016-2020 Teknik sampling menggunakan pusposive sampling sehingga diperoleh 135 sampel perusahaan. Metode pengumpulan data menggunakan dokumentasi. Teknik analisis data menggunakan analisis regresi linier berganda. z Berdasarkan hasil uji hipotesis menunjukkan bahwa signifikansi komite auditor 0,000<0,05, sehingga komite auditor berpengaruh signifikan terhadap penghindaran pajak. Signifikansi kualitas auditor 0,288>0,05, sehingga kualitas auditor tidak berpengaruh signifikan terhadap penghindaran pajak. Signifikansi pertumbuhan penjualan 0,000<0,05, sehingga pertumbuhan penjualan berpengaruh signifikan tehrhadap penghindaran pajak. Koefisien determinasi menunjukkan hasil 0,281 atau 28,1%. Bagi peneliti selanjutnya diharapkan dapat menambah sampel penelitian dengan sektor yang lain agar dapat menggambarkan kondisi perusahaan secara keseluruhan terkait penghindaran pajak.
Management of state budget financing in the aspect of Ukraine’s cooperation with the IMF
Authors:   SESE “The Academy of Financial Management”, KUDRJASHOV Vasyl
Published: July 2023

Introduction. During the war, the tasks of implementing new approaches to managing operations involving the attraction and use of resources within the framework of state budget financing arose. At the same time, the risks of maintaining financial stability and debt sustainability intensified. The resolution of these issues became the subject of cooperation between the state administration bodies of Ukraine and the IMF. Problem Statement. The gaps between the growing expenses of the state budget and their revenue coverage have increased. The result was an increase in the burden on the financing of the state budget, which required making significant changes to the sources of attracting funds and the mechanisms for their implementation. Purpose. Conducting an analysis of changes in the management of the state budget financing of Ukraine during the war in the aspect of Ukraine's cooperation with the IMF. Methods. An analysis of state budget financing during the war and overcoming its consequences was carried out. The main tasks, sources and mechanisms of state budget financing are revealed. The problems of resource attraction from the domestic financial market, as well as financing from external sources, are highlighted. Results. At the initial stage of the war, budget expenditures were significantly increased and changes were made to their structure. At the same time, deficit indicators increased, and its coverage was provided by budget financing operations. Due to the high risks of increasing the non-debt resource (on the basis of privatization and active operations), there arose a need of increasing state borrowing. During the use of internal resources, monetary financing was allowed, which had a negative impact on the dynamics of macroeconomic indicators. External debt obligations are provided on preferential terms and have a long-term nature. The use of such financing made it possible to ensure macroeconomic and financial stability in Ukraine, but was accompanied by an increase in public debt indicators. It is noted that the allocation of domestic state borrowing needs to be reviewed, and external financial assistance is subject to changes in the direction of rationalization and replacement by internal financing. Conclusions. Measures developed within the framework of cooperation between Ukraine and the IMF allow to ensure budgetary capacity and debt sustainability. In order to strengthen them, it is proposed to increase the volume of non-debt resources for financing the budget, to replace monetary financing with non-state sources of its provision, to adopt programs for the gradual restoration of fiscal rules, as well as access to international capital markets, to make changes to the attraction of financial assistance (gradual replacement of it by other sources), adjusting the strategic principles of public debt management, taking into account the effects of the war and the needs of post-war recovery.
Don't worry, tweet happy. Emoji for affective communication on Twitter
Authors: Alloing Camille , Pierre Julien
Published: July 2023

Among the signs circulating on social media platforms, emoji play a special role. Typographic in nature, they are employed by users and community managers alike. Using a mixed-methods approach, combining a mass collection of 134,678 tweets alongside 22 semi-structured interviews with community managers, this article explores how emoji contribute to the production of a relational grammar between organizations and their publics. In following the use of emoji, we highlight the emotional and affective labor of community managers as well as the type of relationships they can build, using “word-images” or “word-emotions”.
Análisis de la comunidad vágil y sésil del intermareal rocoso de cazones, Veracruz, México
Authors: Rodríguez-Muñoz Salvador , Granados-Vargas Natalia , De la Cruz-Francisco Vicencio
Published: July 2023

El intermareal rocoso de Cazones, Veracruz ya cuenta con información de la diversidad biológica, sin embargo, es necesario conocer la estructura comunitaria bajo un enfoque cuantitativo. El objetivo del presente trabajo fue caracterizar la comunidad biológica del intermareal alto, medio y bajo en tres sitios de muestreo. En cada sitio se tendieron dos transectos de 20 m de largo en el nivel alto, medio y bajo del intermareal, y en cada transecto se colocaron a cada 5 m, dos cuadrantes de 50 x 50 cm para estimar el porcentaje de cobertura de especies sésiles y la abundancia de las especies vágiles, así mismo, se estimaron índices de diversidad alfa (riqueza de especies, dominancia, y diversidad verdadera) y se determinaron diferencias significativas entre las franjas intermareales mediante análisis multivariados. Se registraron un total de 110 especies, las cuales están agrupadas en tres reinos: Chromista, Plantae y Animalia, este último reino presentó siete filos, Mollusca fue el más diverso con 22 especies registradas. La estructura comunitaria en el nivel alto fue homogénea y poco diversa en especies y prevaleció la dominancia; bivalvos, balanos y gasterópodos fueron los más conspicuos. Hacia el intermareal medio y bajo, la riqueza de especies fue mayor y la estructura comunitaria fue heterogénea; los pastos marinos y macroalgas presentaron mayor cobertura en el sitio 1 y 2 y disminuyó hacia el nivel bajo, en este lugar la comunidad vágil fue dominante caracterizada por la abundancia de erizos y anémonas. Mientras en el nivel medio y bajo del sitio 3 la comunidad fue equitativa y se caracterizó por presentar la mayor diversidad de especies, las macroalgas fueron el principal componente de la comunidad sésil. La estructura de las comunidades intermareales y las diferencias significativas encontradas en este estudio coinciden a lo reportado para otras costas rocosas.
New records of assassin bugs (Hemiptera: Heteroptera: Reduviidae) for the Dominican Republic
Authors: Perez-Gelabert Daniel E.
Published: July 2023

The Hispaniolan fauna of Reduviidae is only partially known, as it has never been the subject of a comprehensive taxonomic or faunistic inventory. These species belong to Neotropical genera that have Caribbean, Central and South American distributions. In this contribution new records are given for 14 species of assassin bugs belonging to six subfamilies. Among these, seven species are first records from the Dominican Republic and the island of Hispaniola: Bactrodes spinulosus Stål, 1862 (Bactrodinae), Lophoscutus rideri Kormilev, 1988, Lophoscutus rugosipes (Guérin-Méneville, 1857) both belonging to Phymatinae, Leogorrus pallipes Stål, 1872 (Reduviinae), and the Saicinae species Oncerotrachelus nasutus (Bergroth, 1913), Tagalis drakkar Varela & Melo, 2017 and Tagalis grossi Gil-Santana, 2011. With these new records the reported extant fauna of Hispaniolan Reduviidae reaches a total of 46 documented species, which are classified in 25 genera and 10 subfamilies. Additionally, 13 fossil species have been reported from the Dominican amber.
Waltzifiability hypothesis
Authors: Tateishi Angel , Carpenedo Amanda , Lopes Jean
Published: July 2023

We present a composition for solo classical guitar. This work comprises four pieces in 3/4 that share the same harmony, but the composition restrictions differ for each. The results are four distinct "waltz" movements. The main contribution of this work is showing composition not as rooted in inspiration, emotions, or innate talent but as a process of learning, critics, and performance. Also is an example of the paradox of choice, i.e., one may be more creative with less freedom of choice. Of course, in this learning environment, the artistic work becomes the musical discourse based on the interaction between the student/composer, the teacher, and the performer.
BioKnit: development of mycelium paste for use with permanent textile formwork
Authors: Kaiser Romy , Bridgens Ben , Elsacker Elise , Scott Jane
Published: July 2023

This paper presents significant advances in mycelium biofabrication using permanent knitted textile formwork and a new substrate formulation to dramatically improve the mechanical properties of mycelium-textile biocomposites suitable for large-scale components for use in construction. The paper outlines the biofabrication process, detailing the composition ofmycocrete, a viscous mycelium substrate developed for use with permanent knitted formwork, and the injection process required to regulate the filling of slender tubes of fabric with mycocrete. The use of a permanent integrated knitted formwork shows promise as a composite system for use with mycelium to improve mechanical performance and enable complex shapes to be fabricated for lightweight construction. Results of mechanical testing show dramatic improvements in tensile, compressive and flexural strength and stiffness compared to conventional mycelium composites. The testing demonstrates the importance of both the mycocrete paste recipe and the knitted textile formwork. In addition, the paper highlights the advantages of the proposed biofabrication system with reference to theBioKnitprototype: a 1.8 m high freestanding arched dome composed of very slender biohybrid knit-mycelium tubes. This prototype demonstrates the opportunity to utilize the potential for lightweight construction and complex form offered by a textile formwork with low environmental impact mycelium biomaterials. The combination of textiles and mycelium present a compelling new class of textile biohybrid composite materials for new applications within the construction sector.
Technology optimization for patient safety: a blockchain-based anesthesia record system architecture
Authors: Orofino Giambastiani Roberto , Sáenz Rodrigo , Lahitte Guido , Umaran Juan
Published: July 2023

Patient safety is acknowledged as a primary aim of anesthesiology. Anesthesia records constitute the main document of the intraoperative course of anesthesia administration. In this paper, we postulate that anesthesia record systems should be based on an integral tamper-proof design and provide specific technology characteristics to ensure data immutability, accessibility and transparency. Issues and limitations regarding current anesthesia record technologies are reviewed. We introduce a novel anesthesia record system designed for patient safety optimization which integrates dedicated hardware, blockchain technology and decentralized storage solutions. We propose an oracle network in which anesthesiologists run independent Sybil-resistant nodes which broadcast biosensor time series to decentralized storage systems and generate proofs of existence on public blockchains. Records are biometrically signed and incorporate information on the temporo-spatial relation between the anesthetized patient and the professional in charge through a unique personal-transponder wearable device. Compatibility for data science and machine learning implementation are discussed. Finally, we evaluate future impact and technological potential.
Differentiation of peripheral sensory neurons from iPSCs derived from stem cells from human exfoliated deciduous teeth (SHED)
Authors: Oliveira Nathalia C. , Russo Fabiele B. , Beltrão-Braga Patricia C. B.
Published: July 2023

Peripheral nervous system (PNS) sensory alterations are present in several pathologies and syndromes. The use of induced pluripotent stem cell (iPSC) technology is an important strategy to produce sensory neurons in patients who are accomplished in terms of sensory symptoms. The iPSC technology relies on manipulating signaling pathways to resemble what occurs in vivo, and the iPSCs are known to carry a transcriptional memory after reprogramming, which can affect the produced cell. To this date, protocols described for sensory neuron production start using iPSCs derived from skin fibroblasts, which have the same ontogenetic origin as the central nervous system (CNS). Since it is already known that the cells somehow resemble their origin even after cell reprogramming, PNS cells should be produced from cells derived from the neural crest. This work aimed to establish a protocol to differentiate sensory neurons derived from stem cells from human exfoliated deciduous teeth (SHED) with the same embryonic origin as the PNS. SHED-derived iPSCs were produced and submitted to peripheral sensory neuron (PSN) differentiation. Our protocol used the dual-SMAD inhibition method, followed by neuronal differentiation, using artificial neurotrophic factors and molecules produced by human keratinocytes. We successfully established the first protocol for differentiating neural crest and PNS cells from SHED-derived iPSCs, enabling future studies of PNS pathologies.
Kinetics and product identification of water-dissolved nitroguaiacol photolysis under artificial sunlight
Authors: Delić Ajda , Skube Urša , Šala Martin , Kroflič Ana
Published: July 2023

Nitroguaiacols are typical constituents of biomass-burning emissions, including absorbing aerosols which contribute to climate change. Although they are also harmful to humans and plants, their atmospheric fate and lifetimes are still very speculative. Therefore, in this work, the photolysis kinetics of aqueous-phase 4-nitroguaiacol (4NG) and 5-nitroguaiacol (5NG), and the resulting photo-formed products were investigated under artificial sunlight, observing also the effect of sunlight on the absorption properties of the solutions. We found the photolysis of 5NG slower than that of 4NG, whereas the absorbance in the visible range prevailed in the 5NG solutions at the end of experiments. Although we identified dinitroguaiacol as one of the 4NG photolysis products, which increased light absorption of 4NG-containing solutions, considerably more chromophores formed in the 5NG photolyzed solutions, implying its stronger potential for secondary BrC formation in the atmosphere. In general, denitration, carbon loss, hydroxylation, nitration, and carbon gain were characteristic of 4NG phototransformation, while carbon loss, hydroxylation, and carbon gain were observed in the case of 5NG. The photolysis kinetics was found of the first order at low precursor concentrations (<0.45 mM), resulting in their lifetimes in the order of days (125 and 167 h illumination for 4NG and 5NG, respectively), which suggests long-range transport of the investigated compounds in the atmosphere and proposes their use as biomass-burning aerosol tracer compounds.
Progress in drug delivery and diagnostic applications of carbon dots: a systematic review
Authors: Kaurav Hemlata , Verma Dhriti , Bansal Amit , Kapoor Deepak N. , Sheth Sandeep
Published: July 2023

Carbon dots (CDs), which have particle size of less than 10 nm, are carbon-based nanomaterials that are used in a wide range of applications in the area of novel drug delivery in cancer, ocular diseases, infectious diseases, and brain disorders. CDs are biocompatible, eco-friendly, easy to synthesize, and less toxic with excellent chemical inertness, which makes them very good nanocarrier system to deliver multi-functional drugs effectively. A huge number of researchers worldwide are working on CDs-based drug delivery systems to evaluate their versatility and efficacy in the field of pharmaceuticals. As a result, there is a tremendous increase in our understanding of the physicochemical properties, diagnostic and drug delivery aspects of CDs, which consequently has led us to design and develop CDs-based theranostic system for the treatment of multiple disorders. In this review, we aim to summarize the advances in application of CDs as nanocarrier including gene delivery, vaccine delivery and antiviral delivery, that has been carried out in the last 5 years.
Natural Climate Solutions must embrace multiple perspectives to ensure synergy with sustainable development
Authors: Waring Bonnie G. , Gurgel Angelo , Köberle Alexandre C. , Paltsev Sergey , Rogelj Joeri
Published: July 2023

To limit global warming to well below 2°C, immediate emissions reductions must be coupled with active removal of greenhouse gases from the atmosphere. “Natural Climate Solutions” (NCS) achieve atmospheric CO2 reduction through the conservation, restoration, or altered management of natural ecosystems, with enormous potential to deliver “win-win-win” outcomes for climate, nature and society. Yet the supply of high-quality NCS projects does not meet market demand, and projects already underway often fail to deliver their promised benefits, due to a complex set of interacting ecological, social, and financial constraints. How can these cross-sectoral challenges be surmounted? Here we draw from expert elicitation surveys and workshops with professionals across the ecological, sociological, and economic sciences, evaluating differing perspectives on NCS, and suggesting how these might be integrated to address urgent environmental challenges. We demonstrate that funders” perceptions of operational, political, and regulatory risk strongly shape the kinds of NCS projects that are implemented, and the locations where they occur. Because of this, greenhouse gas removal through NCS may fall far short of technical potential. Moreover, socioecological co-benefits of NCS are unlikely to be realized unless the local communities engaged with these projects are granted ownership over implementation and outcomes.
Automated T1 and T2 mapping segmentation on cardiovascular magnetic resonance imaging using deep learning
Authors: Kalapos András , Szabó Liliána , Dohy Zsófia , Kiss Máté , Merkely Béla , Gyires-Tóth Bálint , Vágó Hajnalka
Published: July 2023

Introduction

Structural and functional heart abnormalities can be examined non-invasively with cardiac magnetic resonance imaging (CMR). Thanks to the development of MR devices, diagnostic scans can capture more and more relevant information about possible heart diseases. T1 and T2 mapping are such novel technology, providing tissue specific information even without the administration of contrast material. Artificial intelligence solutions based on deep learning have demonstrated state-of-the-art results in many application areas, including medical imaging. More specifically, automated tools applied at cine sequences have revolutionized volumetric CMR reporting in the past five years. Applying deep learning models to T1 and T2 mapping images can similarly improve the efficiency of post-processing pipelines and consequently facilitate diagnostic processes.

Methods

In this paper, we introduce a deep learning model for myocardium segmentation trained on over 7,000 raw CMR images from 262 subjects of heterogeneous disease etiology. The data were labeled by three experts. As part of the evaluation, Dice score and Hausdorff distance among experts is calculated, and the expert consensus is compared with the model’s predictions.

Results

Our deep learning method achieves 86% mean Dice score, while contours provided by three experts on the same data show 90% mean Dice score. The method’s accuracy is consistent across epicardial and endocardial contours, and on basal, midventricular slices, with only 5% lower results on apical slices, which are often challenging even for experts.

Conclusions

We trained and evaluated a deep learning based segmentation model on 262 heterogeneous CMR cases. Applying deep neural networks to T1 and T2 mapping could similarly improve diagnostic practices. Using the fine details of T1 and T2 mapping images and high-quality labels, the objective of this research is to approach human segmentation accuracy with deep learning.
Sepsis-Induced myocardial dysfunction: heterogeneity of functional effects and clinical significance
Authors: Shvilkina Tatyana , Shapiro Nathan
Published: July 2023

Sepsis is a life-threatening disease state characterized by organ dysfunction and a dysregulated response to infection. The heart is one of the many organs affected by sepsis, in an entity termed sepsis-induced cardiomyopathy. This was initially used to describe a reversible depression in ejection fraction with ventricular dilation but advances in echocardiography and introduction of new techniques such as speckle tracking have led to descriptions of other common abnormalities in cardiac function associated with sepsis. This includes not only depression of systolic function, but also supranormal ejection fraction, diastolic dysfunction, and right ventricular dysfunction. These reports have led to inconsistent definitions of sepsis-induced cardiomyopathy. Just as there is heterogeneity among patients with sepsis, there is heterogeneity in the cardiac response; thus resuscitating these patients with a single approach is likely suboptimal. Many factors affect the heart in sepsis including inflammatory mediators, catecholamine responsiveness, and pathogen related toxins. This review will discuss different functional effects characterized by echocardiographic changes in sepsis and their prognostic and management implications.
Mitochondrial epigenetics in aging and cardiovascular diseases
Authors: Mongelli Alessia , Mengozzi Alessandro , Geiger Martin , Gorica Era , Mohammed Shafeeq Ahmed , Paneni Francesco , Ruschitzka Frank , Costantino Sarah
Published: July 2023

Mitochondria are cellular organelles which generate adenosine triphosphate (ATP) molecules for the maintenance of cellular energy through the oxidative phosphorylation. They also regulate a variety of cellular processes including apoptosis and metabolism. Of interest, the inner part of mitochondria—the mitochondrial matrix—contains a circular molecule of DNA (mtDNA) characterised by its own transcriptional machinery. As with genomic DNA, mtDNA may also undergo nucleotide mutations that have been shown to be responsible for mitochondrial dysfunction. During physiological aging, the mitochondrial membrane potential declines and associates with enhanced mitophagy to avoid the accumulation of damaged organelles. Moreover, if the dysfunctional mitochondria are not properly cleared, this could lead to cellular dysfunction and subsequent development of several comorbidities such as cardiovascular diseases (CVDs), diabetes, respiratory and cardiovascular diseases as well as inflammatory disorders and psychiatric diseases. As reported for genomic DNA, mtDNA is also amenable to chemical modifications, namely DNA methylation. Changes in mtDNA methylation have shown to be associated with altered transcriptional programs and mitochondrial dysfunction during aging. In addition, other epigenetic signals have been observed in mitochondria, in particular the interaction between mtDNA methylation and non-coding RNAs. Mitoepigenetic modifications are also involved in the pathogenesis of CVDs where oxygen chain disruption, mitochondrial fission, and ROS formation alter cardiac energy metabolism leading to hypertrophy, hypertension, heart failure and ischemia/reperfusion injury. In the present review, we summarize current evidence on the growing importance of epigenetic changes as modulator of mitochondrial function in aging. A better understanding of the mitochondrial epigenetic landscape may pave the way for personalized therapies to prevent age-related diseases.
Can water resource tax reform improve the environmental performance of enterprises? evidence from China’s high water-consuming enterprises
Authors: Liu Chenyu
Published: July 2023

Water scarcity and pollution have become a global problem, especially in China. Whether China can solve the water resources dilemma is closely related to its ability to achieve high-quality development. At present, studies on China’s water resources policy are relatively few and all of them are theoretical interpretations or regional studies. There is little literature examining the impact of China’s water resources policy on enterprises and its mechanisms. Therefore, this paper takes China’s water resource tax reform in 2017 as a quasi-natural event and constructs a difference-in-differences model to investigate the micro-governance effects of water resource tax reform based on panel data of high water-consuming enterprises listed in Shanghai and Shenzhen A-shares between 2012 and 2020. The findings of the study are as follows. Firstly, the water resource tax reform significantly improves the environmental performance of water-intensive enterprises. Secondly, through the mechanism test the author finds that water resource tax reform can promote the research and development of green invention patents in companies, which in turn improves their environmental performance. However, water resource tax showed no significant effect on green utility model patents. Finally, taking into account heterogeneity, this paper points out that the impact of the reform is more prominent in water-scarce regions and among large-scale enterprises. This paper provides experience and evidence for the promotion of water resource tax reform and inspires the author to give some policy recommendations. In the future, China should continue to implement water resource tax policy and increase technical and financial support to enterprises for green innovation.
An immuno-lipidomic signature revealed by metabolomic and machine-learning approaches in labial salivary gland to diagnose primary Sjögren’s syndrome
Authors: Urbanski Geoffrey , Chabrun Floris , Delattre Estelle , Lacout Carole , Davidson Brittany , Blanchet Odile , Chao de la Barca Juan Manuel , Simard Gilles , Lavigne Christian , Reynier Pascal
Published: July 2023

Introduction

Assessing labial salivary gland exocrinopathy is a cornerstone in primary Sjögren’s syndrome. Currently this relies on the histopathologic diagnosis of focal lymphocytic sialadenitis and computing a focus score by counting lym=phocyte foci. However, those lesions represent advanced stages of primary Sjögren’s syndrome, although earlier recognition of primary Sjögren’s syndrome and its effective treatment could prevent irreversible damage to labial salivary gland. This study aimed at finding early biomarkers of primary Sjögren’s syndrome in labial salivary gland combining metabolomics and machine-learning approaches.

Methods

We used a standardized targeted metabolomic approach involving high performance liquid chromatography coupled with mass spectrometry among newly diagnosed primary Sjögren’s syndrome (n=40) and non- primary Sjögren’s syndrome sicca (n=40) participants in a prospective cohort. A metabolic signature predictive of primary Sjögren’s syndrome status was explored using linear (logistic regression with elastic-net regularization) and non-linear (random forests) machine learning architectures, after splitting the data set into training, validation, and test sets.

Results

Among 126 metabolites accurately measured, we identified a discriminant signature composed of six metabolites with robust performances (ROC-AUC = 0.86) for predicting primary Sjögren’s syndrome status. This signature included the well-known immune-metabolite kynurenine and five phospholipids (LysoPC C28:0; PCaa C26:0; PCaaC30:2; PCae C30:1, and PCaeC30:2). It was split into two main components: the first including the phospholipids was related to the intensity of lymphocytic infiltrates in salivary glands, while the second represented by kynurenine was independently associated with the presence of anti-SSA antibodies in participant serum.

Conclusion

Our results reveal an immuno-lipidomic signature in labial salivary gland that accurately distinguishes early primary Sjögren’s syndrome from other causes of sicca symptoms.
Tracing the invertebrate herpesviruses in the global sequence datasets
Authors: Rosani Umberto , Gaia Morgan , Delmont Tom O. , Krupovic Mart
Published: July 2023

The family of Malacoherpesviridae is currently represented by only two viruses infecting molluscs, Ostreid herpesvirus 1 (OsHV-1) and Haliotid herpesvirus 1 (HaHV-1), both causing detrimental infections in aquaculture species. Malacoherpesvirus-like sequences were also detected through genome sequencing projects in amphioxus (Branchiostoma species) and annelid worm (Capitella teleta), suggesting the existence of a hidden diversity of malacoherpesviruses in aquatic animals. Here, to extend the knowledge on malacoherpesvirus diversity, we searched for the presence of malacoherpesvirus relatives in genomic, transcriptomic and metagenomic datasets, including from the Tara Oceans expedition, and report 4 novel malacoherpesvirus-like genomes (MalacoHV1-4). Genomic analysis suggested gastropods and bivalves as the most probable hosts for these new malacoherpesviruses. Phylogenetic analysis based on the family B DNA polymerase placed the novel MalacoHV1 and MalacoHV3 as sister lineages of OsHV-1 and HaHV-1, respectively, whereas MalacoHV2 and MalacoHV4 showed higher divergence. The viral genome found associated with amphioxus together with MalacoHV4 formed a sister clade to the mollusc and annelid malacoherpesviruses, suggesting an early divergence of the two virus assemblages. In conclusion, although relatively rare in the available sequence databases, the previously undescribed malacoherpesviruses, MalacoHV1-4, circulate in aquatic ecosystems and should be considered as possible emerging viruses under changing environmental conditions.
Impact of Microsetella norvegica on carbon flux attenuation and as a secondary producer during the polar night in the subarctic Porsangerfjord
Authors: Mooney Benjamin Paul , Iversen Morten Hvitfeldt , Norrbin Maria Fredrika
Published: July 2023

It is known that Microsetella norvegica feed on phytoplankton and provide an important link to higher trophic levels in Arctic fjords, such as fish sprat (Sprattus sprattus) and three-spined stickleback (Gasterosteus aculeatus). It has recently been suggested that M. norvegica may also contribute substantially to carbon flux attenuation during periods of high abundance. However, we still know very little about how seasonal variations in abundance and vertical distribution of M. norvegica impact the efficiency of the biological carbon pump in Arctic fjords. We investigated the role of Microsetella norvegica, a small harpacticoid copepod, for particulate organic carbon flux attenuation via aggregate feeding in a subarctic fjord. We quantified the vertical distribution and abundance of M. norvegica, phytoplankton, and marine snow simultaneously with a Digital Autonomous Video Plankton Recorder in Porsangerfjord, northern Norway, between August 2013 and November 2014. We estimated the highest abundance of M. norvegica as 4.86x106 individuals m-2 in October. Our results suggest that M. norvegica preferred diatoms over both marine snow and the prymnesiophyte Phaeocystis pouchetii during euphotic bloom conditions. However, during oligotrophic conditions when phytoplankton were scarce, M. norvegica switched to marine snow as a food source. M. norvegica has the potential to explain 1.4% and 0.29% of the total carbon flux attenuation in October and November, respectively. These results suggest that small copepods feed on settling detritus when no alternative food is available. Detritus feeding by M. norvegica may have an ecological impact during the polar night, both via direct carbon flux attenuation, but also as secondary producers in periods with low primary production. Currently small copepods such as M. norvegica are not included in carbon budgets or large-scale modelling, but considering their potentially high abundance they may represent an important but overlooked pathway in both the carbon cycle and trophic level interactions.
A simulation study of in-beam visualization system for proton therapy by monitoring scattered protons
Authors: Sato Shogo , Yokokawa Hiromu , Hosobuchi Mana , Kataoka Jun
Published: July 2023

Recently, in-beam positron emission tomography (PET) has been actively researched for reducing biological washout effects and dose monitoring during irradiation. However, the positron distribution does not precisely reflect the dose distribution since positron production and ionization are completely different physical processes. Thus, a novel in-beam system was proposed to determine proton dose range by measuring scattered protons with dozens of scintillation detectors surrounding the body surface. While previous studies conducted a preliminary experiment with a simple phantom, we simulated more complex situations in this paper. Especially, we conducted three stepwise simulation studies to demonstrate the feasibility of the proposed method. First, a simple rectangular phantom was reproduced on simulation and irradiated with protons for obtaining current values and Monte Carlo (MC) dose. Next, we trained a deep learning model to estimate 2-dimensional-dose range (2D-DL dose) from measured current values for simulation (A). We simulated plastic scintillators as detectors to measure the scattered protons. Second, a rectangular phantom with an air layer was used, and 3D-DL dose was estimated in simulation (B). Finally, a cylindrical phantom that mimics the human body was used for confirming the estimation quality of the simulation (C). Consequently, the position of the Bragg peak was estimated with an error of 1.0 mm in simulation (A). In addition, the position of the air layer, as well as the verifying peak position with an error of 2.1 mm, was successfully estimated in simulation (B). Although the estimation error of the peak position was 12.6 mm in simulation (C), the quality was successfully further improved to 9.3 mm by incorporating the mass density distribution obtained from the computed tomography (CT). These simulation results demonstrated the potential of the as-proposed verification system. Additionally, the effectiveness of CT utilization for estimating the DL dose was also indicated.
Short-term application of diquafosol ophthalmic solution benefits children with dry eye wearing orthokeratology lens
Authors: Yang Yuanfang , Wu Qinghui , Tang Yao , Wu Haoran , Luo Zhiwei , Gao Wenyu , Hu Ziqi , Hou Lijun , Wang Min , Yang Zhikuan , Li Xiaoning
Published: July 2023

Purpose

This aim of this study was to evaluate the effect of 3% Diquafosol Ophthalmic Solution (DQS) on children with dry eye from wearing overnight orthokeratology (OrthoK) lenses.

Methods

Myopic children aged 8–18 years with dry eye syndrome were enrolled in this prospective observational study, and they were grouped according to their OrthoK treatment history for at least 1 year. All participants received DQS 4 times per day for 1 month. The following indicators were measured at baseline 1 month after treatment: the Dry Eye Questionnaire-5 (DEQ-5), non-invasive tear meniscus height (TMH), non-invasive tear film break-up time (first and average, NIBUT-F and NIBUT-A), meibomian gland score (MG score), conjunctival hyperemia redness score (R-scan), and blink pattern analysis.

Results

A total of 104 participants (189 eyes) including 40 OrthoK wearers (72 eyes) and 64 Orthok candidates (117 eyes) completed the study. Of all, after DQS treatment for 1 month, DEQ-5 scores reduced from 5.54 ± 3.25 to 3.85 ± 2.98 (t = −3.36, p = 0.00). TMH increased from 0.20 ± 0.05 mm to 0.21 ± 0.05 mm (t = 2.59, p = 0.01), NIBUT-F and NIBUT-A were prolonged from 6.67 ± 4.71 s to 10.32 ± 6.19 s and from 8.86 ± 5.25 s to 13.30 ± 6.03 s (all p = 0.00), respectively. R-scan decreased from 0.69 ± 0.28 to 0.50 ± 0.25 (t = −9.01, p = 0.00). Upper MG scores decreased from 1.04 ± 0.32 to 0.97 ± 0.36 (t = −2.14, p = 0.03). Lower MG scores, partial blink rate, partial blinks, and total blinks did not change significantly. Both break-up time (BUT) and R-scan improved significantly after DQS treatment for 1 month (all p = 0.00) in OrthoK candidates and OrthoK wearers. Among the OrthoK wearers, TMH and dry eye symptoms increased significantly (all p = 0.00) but did not increase in OrthoK candidates (p > 0.05). There were no adverse events related to DQS.

Conclusion

Diquafosol Ophthalmic Solution was effective for children wearing overnight orthokeratology in relieving dry eye symptoms and improving ocular surface parameters, which may help improve children's OrthoK wearing tolerance and compliance.
Effect of long-term rotation on astigmatism following EVO-toric intraocular collamer lens implantation
Authors: Chen Xun , Miao Huamao , Cheng Mingrui , Lin I-Chun , Li Boliang , Jiang Yinjie , Lei Yadi , Wang Xiaoying , Zhou Xingtao
Published: July 2023

Objective

To evaluate the effect of long-term rotation on astigmatism following Evolution-toric intraocular collamer lens (EVO-TICL) implantation.

Methods

Forty eyes of 22 patients were enrolled in this prospective study. Visual acuity, refractive parameters, and axial position of the EVO-TICL by OPD-Scan III aberrometer were measured preoperatively, 1 month and 3 years postoperatively.

Results

Last visit, the safety index was 1.32 ± 0.15 and the efficacy index was 1.01 ± 0.23. The best-fitting curve of the attempted versus achieved correction was y = 0.9751x + 0.001. The mean spherical equivalent (SE) decreased from −8.94 ± 2.72D preoperatively to 0.06 ± 0.24D and − 0.36 ± 0.46D 1 month and 3 years postoperatively, respectively. The mean target and surgical induced astigmatism were 1.55 ± 0.61D and 1.67 ± 0.94D 3 years postoperatively. The average expected axis of the TICL was-1.15 ± 9.07 (−21–19°). One month and 3 years postoperatively, the average actual axis was −0.70 ± 9.86 (−20–20°) and − 0.35 ± 11.72 (−25–30°), respectively. The absolute rotation of the TICL was 3.70 ± 4.42 (0–22°) and 6.00 ± 6.70 (0–32°) 1 month and 3 years postoperatively, respectively (p < 0.001). The expected astigmatism was −0.10 ± 0.12D, and the mean actual astigmatism was −0.21 ± 0.30D and − 0.44 ± 0.45D 1 month and 3 years postoperatively, respectively. The mean absolute rotation without postoperative astigmatism was 3.73 ± 2.69 (0–9°) and 1.67 ± 1.66 (0–5°) for low (<2D) and high (≥2D) astigmatic TICL, respectively (p < 0.05).

Conclusion

EVO-TICL implantation is safe and effective, with good predictability and stability. OPD-Scan is a fast device to detect the axial position of the TICL without mydriasis, and the axial position is relatively stable in the long term postoperatively. A slight rotation of low-astigmatic TICL may not cause postoperative astigmatism, whereas rotation of the high-astigmatic TICL may cause it.
HMGB1, a potential regulator of tumor microenvironment in KSHV-infected endothelial cells
Authors: Lee Myung-Ju , Park Joohee , Choi Seokjoo , Yoo Seung-Min , Park Changhoon , Kim Hong Seok , Lee Myung-Shin
Published: July 2023

High-mobility group box 1 (HMGB1) is a protein that binds to DNA and participates in various cellular processes, including DNA repair, transcription, and inflammation. It is also associated with cancer progression and therapeutic resistance. Despite its known role in promoting tumor growth and immune evasion in the tumor microenvironment, the contribution of HMGB1 to the development of Kaposi’s sarcoma (KS) is not well understood. We investigated the effect of HMGB1 on KS pathogenesis using immortalized human endothelial cells infected with Kaposi’s sarcoma-associated human herpes virus (KSHV). Our results showed that a higher amount of HMGB1 was detected in the supernatant of KSHV-infected cells compared to that of mock-infected cells, indicating that KSHV infection induced the secretion of HMGB1 in human endothelial cells. By generating HMGB1 knockout clones from immortalized human endothelial cells using CRISPR/Cas9, we elucidated the role of HMGB1 in KSHV-infected endothelial cells. Our findings indicate that the absence of HMGB1 did not induce lytic replication in KSHV-infected cells, but the cell viability of KSHV-infected cells was decreased in both 2D and 3D cultures. Through the antibody array for cytokines and growth factors, CXCL5, PDGF-AA, G-CSF, Emmprin, IL-17A, and VEGF were found to be suppressed in HMGB1 KO KSHV-infected cells compared to the KSHV-infected wild-type control. Mechanistically, phosphorylation of p38 would be associated with transcriptional regulation of CXCL5, PDGF-A and VEGF. These observations suggest that HMGB1 may play a critical role in KS pathogenesis by regulating cytokine and growth factor secretion and emphasize its potential as a therapeutic target for KS by modulating the tumor microenvironment.
Chitosan, polyethylene oxide/polycaprolactone electrospun core/shell nanofibrous mat containing rosuvastatin as a novel drug delivery system for enhancing human mesenchymal stem cell osteogenesis
Authors: Ghasemvand Fariba , Kabiri Mahboubeh , Hassan-Zadeh Vahideh , Simchi Abdolreza
Published: July 2023

Introduction: Due to the potential positive effects of rosuvastatin (RSV) on human mesenchymal stem cells (MSCs) osteogenesis and new bone regeneration, it is crucial to develop a suitable carrier that can effectively control the release profile of RSV. The primary objective of this study was to introduce a novel drug delivery system based on core/shell nanofibrous structures, enabling a sustained release of RSV.

Methods: To achieve this, coaxial electrospinning was employed to fabricate chitosan (CS)+polyethylene oxide (PEO)/polycaprolactone (PCL) nanofibrous mats, wherein RSV was incorporated within the core of nanofibers. By optimizing the relevant parameters of the electrospinning process, the mats’ surface was further modified using plasma treatment. The fibers’ shape, structure, and thermal stability were characterized. The wettability, and degradation properties of the fabricated mats were also examined. In vitro studies were conducted to examine the release behavior of RSV. Additionally, the capability of MSCs to survive and differentiate into osteocytes when cultured on nanofibers containing RSV was evaluated.

Results: Results demonstrated the successful fabrication of CS + PEO + RSV/PCL core/shell mats with a core diameter of approximately 370 nm and a shell thickness of around 70 nm under optimized conditions. Plasma treatment was found to enhance the wettability and drug-release behavior of the mats. The nanofibrous structure, serving as a carrier for RSV, exhibited increased proliferation of MSCs and enhanced osteogenic differentiation.

Conclusion: Therefore, it can be concluded that CS + PEO + RSV/PCL core/shell nanofibrous structure can be utilized as a sustained-release platform for RSV over an extended period, making it a promising candidate for guided bone regeneration.
What can challenging reproductive contexts tell us about the rat’s maternal behavior?
Authors: Agrati Daniella , Uriarte Natalia
Published: July 2023

Maternal behavior in mammals encompasses a complex repertoire of activities that ensure the survival of the offspring and shape their neural and behavioral development. The laboratory rat has been employed as a classic model for investigating maternal behavior, and recently with the use of advanced techniques, the knowledge of its neural basis has been expanded significantly. However, the standard laboratory testing conditions in which rats take care of a single litter impose constraints on the study of maternal flexibility. Interestingly, the reproductive characteristics of this species, including the existence of a fertile postpartum estrus, allow us to study maternal behavior in more complex and ethologically relevant contexts, even in laboratory settings. Here we review how maternal and sexual motivations interact during the postpartum estrus, shaping the behavioral response of females according to the presence of the pups and males. Next, we describe how impregnation during the postpartum estrus creates a new reproductive context in which mothers simultaneously care for two successive litters, adapting their responses to different behavioral and physiological demands of pups. These findings illustrate the behavioral adaptability of maternal rats to pups’ needs and the presence of other reinforcers, as well as its dependence on the context. In our view, future perspectives in the field, by incorporating the use of cutting-edge techniques, should analyze maternal flexibility and its neural substrates in models that incorporate complex and challenging contexts. This approach would allow a more comprehensive understanding of brain circuits involved in the adaptive and flexible nature of parenting.