bluuebunny/crossref_metadata_2025 huggingface dataset:
Big Data Technology in Construction Safety Management: Application Status, Trend and Challenge
Authors: Meng Qingfeng , Peng Qiyuan , Li Zhen , Hu Xin
Published: April 2022

The construction industry is a high-risk industry with many safety accidents. The popularity of Internet information technology has led to an explosion in the amount of data obtained in various engineering fields, and it is of necessary significance to explore the current situation of the application of big data technology in construction safety management. This paper systematically reviews 66 articles closely related to the research topic and objectives, describes the current status of big data application to various construction safety issues from the perspectives of both big data collection and big data analysis for engineering and construction projects, and categorically lists the breakthrough results of big data analysis technology in improving construction safety. Finally, the trends and challenges of big data in the field of construction safety are discussed in three directions: the application of big data to worker behavior, the prospect of integrating big data technologies, and the integration of big data technologies with construction management. The aim of this paper is to demonstrate the current state of research on big data technology fueling construction safety management, providing valuable insight into improving safety at engineering construction sites and providing guidance for future research in this field.
An Explorative Study of the Incidental High Renal Excretion of [18F]PSMA-1007 for Prostate Cancer PET/CT Imaging
Authors: Allach Youssra , Banda Amina , van Gemert Willemijn , de Groot Michel , Derks Yvonne , Schilham Melline , Hoepping Alexander , Perk Lars , Gotthardt Martin , Janssen Marcel , Nagarajah James , Privé Bastiaan M.
Published: April 2022

Positron emission tomography (PET) of prostate-specific membrane antigen (PSMA) allows for accurate diagnosis and staging of prostate cancer (PCa). Compared to other PSMA PET tracers available, [18F]PSMA-1007 is predominantly excreted via the hepatobiliary tract resulting in low renal excretion which improves evaluation of the pelvic area. However, some patients do show high urinary uptake of [18F]PSMA-1007. The present study aimed to investigate this sudden high urinary uptake of [18F]PSMA-1007 by evaluating [18F]PSMA-1007 PET scans from PCa patients. In this single-center retrospective study, patients that underwent [18F]PSMA-1007 PET imaging between July 2018 and January 2021 were included. Data regarding the individual patient characteristics, scan acquisition and batch production were analyzed. To determine the urinary excretion of [18F]PSMA-1007, a region of interest was drawn in the bladder, and standardized uptake values (SUVs) were calculated and compared to SUVs in the prostate. An SUVmax of >10 was considered high urinary excretion, an SUVmax 7.5–10 intermediate and an SUVmax < 7.5 low urinary excretion. A total of 344 patients underwent [18F]PSMA-1007 PET/CT imaging, with 37 patients receiving three or more [18F]PSMA-1007 PET/CT scans. The mean SUVmean and SUVmax of the bladder were 3.9 (SD 2.9) and 5.9 (SD 4.2), respectively. Fourteen percent of patients showed high urinary uptake of [18F]PSMA-1007. Twelve of the thirty-seven patients (32.4%) that had multiple scans showed a varying urinary uptake of [18F]PSMA-1007 per PSMA PET/CT scan. In terms of patient characteristics, risk factors, medication and blood laboratory results, no significant influencing variables were found. Nor was there a difference observed in the batch size and the mean radiochemical purity of PSMA-1007 for high- and low-excreting patients. However, the bladder volume affected the mean SUVmax in the bladder significantly, with higher SUVs in lower bladder volumes. In this study, we observed that a higher SUV in the urinary tract seemed to occur in patients with low bladder volume. A prospective study is needed to corroborate this hypothesis.
Genotyping of Circulating Free DNA Enables Monitoring of Tumor Dynamics in Synovial Sarcomas
Authors: Eisenhardt Anja E. , Brugger Zacharias , Lausch Ute , Kiefer Jurij , Zeller Johannes , Runkel Alexander , Schmid Adrian , Bronsert Peter , Wehrle Julius , Leithner Andreas , Liegl-Atzwanger Bernadette , Giunta Riccardo E. , Eisenhardt Steffen U. , Braig David
Published: April 2022

Background: Synovial sarcoma (SS) is a malignant soft tissue tumor of mesenchymal origin that frequently occurs in young adults. Translocation of the SYT gene on chromosome 18 to the SSX genes on chromosome X leads to the formation of oncogenic fusion genes, which lead to initiation and proliferation of tumor cells. The detection and quantification of circulating tumor DNA (ctDNA) can serve as a non-invasive method for diagnostics of local or distant tumor recurrence, which could improve survival rates due to early detection. Methods: We developed a subtype-specific targeted next-generation sequencing (NGS) approach specifically targeting SS t(X;18)(p11;q11), which fuses SS18 (SYT) in chromosome 18 to SSX1 or SSX2 in chromosome x, and recurrent point mutations. In addition, patient-specific panels were designed from tumor exome sequencing. Both approaches were used to quantify ctDNA in patients’ plasma. Results: The subtype-specific assay allowed detection of somatic mutations from 25/25 tumors with a mean of 1.68 targetable mutations. The minimal limit of detection was determined at a variant allele frequency of 0.05%. Analysis of 29 plasma samples from 15 tumor patients identified breakpoint ctDNA in 6 patients (sensitivity: 40%, specificity 100%). The addition of more mutations further increased assay sensitivity. Quantification of ctDNA in plasma samples (n = 11) from one patient collected over 3 years, with a patient-specific panel based on tumor exome sequencing, correlated with the clinical course, response to treatment and tumor volume. Conclusions: Targeted NGS allows for highly sensitive tumor profiling and non-invasive detection of ctDNA in SS patients, enabling non-invasive monitoring of tumor dynamics.
Comparison of Intraductal RFA Plus Stent versus Stent-Only Treatment for Unresectable Perihilar Cholangiocarcinoma—A Systematic Review and Meta-Analysis
Authors: de Jong David M. , Fritzsche Jeska A. , Audhoe Amber S. , Yi Suzanne S. L. , Bruno Marco J. , Voermans Rogier P. , van Driel Lydi M. J. W.
Published: April 2022

Background: One of the cornerstones of palliative treatment for unresectable perihilar cholangiocarcinoma is biliary stent placement in order to restore biliary drainage. In this review, the potential added value of RFA with stent placement in comparison to stent placement alone in patients with unresectable perihilar cholangiocarcinoma is analyzed. Methods: We performed a comprehensive online search for relevant articles in November 2021 (PROSPERO ID: CRD42021288180). The primary endpoint was difference in overall survival. Secondary endpoints included overall survival, stent patency and complications. Only studies comparing survival after RFA + stent placement with stent placement alone were included in the meta-analysis. Non-comparative studies or comparative studies describing stent patency only were included in the systematic review. Results: A total of nine studies, including 217 patients with pCCA who underwent RFA + stent placement and 294 patients who underwent stent-only treatment, met the inclusion criteria for the primary endpoint analysis. Direct comparison between the two treatment groups showed a significantly longer overall survival for RFA + stent treatment, with a pooled HR of 0.65 [95% CI, 0.50–0.84, I2 = 38%]. When all eligible studies were included, RFA + stent treatment revealed an overall survival of 9.5 months [95% CI, 6.3–12.6], whereas survival for stent-only treatment was 7.0 months [95% CI, 5.7–8.2]. Due to the heterogeneity of the data, no pooled data analysis could be performed on stent patency or complications. Conclusions: RFA + stent placement displays promising potential to prolong survival. However, further research incorporating confounding factors like use of palliative chemotherapy is necessary in order to validate these findings.
Circulating Cell-Free DNA Assessment in Biofluids from Children with Neuroblastoma Demonstrates Feasibility and Potential for Minimally Invasive Molecular Diagnostics
Authors: Lodrini Marco , Wünschel Jasmin , Thole-Kliesch Theresa M. , Grimaldi Maddalena , Sprüssel Annika , Linke Rasmus B. , Hollander Jan F. , Tiburtius Daniela , Künkele Annette , Schulte Johannes H. , Lankes Erwin , Elgeti Thomas , Hundsdörfer Patrick , Astrahantseff Kathy , Simon Thorsten , Eggert Angelika , Deubzer Hedwig E.
Published: April 2022

Liquid biopsy strategies in pediatric patients are challenging due to low body weight. This study investigated cfDNA size distribution and concentration in blood, bone marrow, cerebrospinal fluid, and urine from 84 patients with neuroblastoma classified as low (n = 28), intermediate (n = 6), or high risk (n = 50) to provide key data for liquid biopsy biobanking strategies. The average volume of blood and bone marrow plasma provided ranged between 1 and 2 mL. Analysis of 637 DNA electropherograms obtained by Agilent TapeStation measurement revealed five different major profiles and characteristic DNA size distribution patterns for each of the biofluids. The proportion of samples containing primarily cfDNA was, at 85.5%, the highest for blood plasma. The median cfDNA concentration amounted to 6.28 ng/mL (blood plasma), 58.2 ng/mL (bone marrow plasma), 0.08 ng/mL (cerebrospinal fluid), and 0.49 ng/mL (urine) in samples. Meta-analysis of the dataset demonstrated that multiple cfDNA-based assays employing the same biofluid sample optimally require sampling volumes of 1 mL for blood and bone marrow plasma, 2 mL for cerebrospinal fluid, and as large as possible for urine samples. A favorable response to treatment was associated with a rapid decrease in blood-based cfDNA concentration in patients with high-risk neuroblastoma. Blood-based cfDNA concentration was not sufficient as a single parameter to indicate high-risk disease recurrence. We provide proof of concept that monitoring neuroblastoma-specific markers in very small blood volumes from infants is feasible.
The Significance of Systemic Inflammation Markers in Intrahepatic Recurrence of Early-Stage Hepatocellular Carcinoma after Curative Treatment
Authors: Bae Bong Kyung , Park Hee Chul , Yoo Gyu Sang , Choi Moon Seok , Oh Joo Hyun , Yu Jeong Il
Published: April 2022

Systemic inflammatory markers (SIMs) are known to be associated with carcinogenesis and prognosis of hepatocellular carcinoma (HCC). We evaluated the significance of SIMs in intrahepatic recurrence (IHR) of early-stage HCC after curative treatment. This study was performed using prospectively collected registry data of newly diagnosed, previously untreated HCC between 2005 and 2017 at a single institution. Inclusion criteria were patients with Barcelona Clinic Liver Cancer stage 0 or A, who underwent curative treatment. Pre-treatment and post-treatment values of platelet, neutrophil, lymphocyte, monocyte, neutrophil/lymphocyte ratio (NLR), platelet/lymphocyte ratio (PLR), and lymphocyte/monocyte ratio (LMR) were analyzed with previously well-known risk factors of HCC to identify factors associated with IHR-free survival (IHRFS), early IHR, and late IHR. Of 4076 patients, 2142 patients (52.6%) experienced IHR, with early IHR in 1018 patients (25.0%) and late IHR in 1124 patients (27.6%). Pre-treatment platelet count and PLR and post-treatment worsening of NLR, PLR, and LMR were independently associated with IHRFS. Pre-treatment platelet count and post-treatment worsening of NLR, PLR, and LMR were significantly related to both early and late IHR. Pre-treatment values and post-treatment changes in SIMs were significant factors of IHR in early-stage HCC, independent of previously well-known risk factors of HCC.
The Role of Treatment Sequencing with Immune-Checkpoint Inhibitors and BRAF/MEK Inhibitors for Response and Survival of Patients with BRAFV600-Mutant Metastatic Melanoma—A Retrospective, Real-World Cohort Study
Authors: Haist Maximilian , Stege Henner , Ebner Ronja , Fleischer Maria Isabel , Loquai Carmen , Grabbe Stephan
Published: April 2022

The advent of immune-checkpoint inhibitors (CPI) and BRAF/MEK-directed targeted therapy (TT) has improved the treatment landscape of patients with BRAFV600-mutant metastatic melanoma. While TT allows for rapid disease control, the development of secondary TT resistance limits the duration of responses. Responses to CPI have a slower onset but can be durable in a subset of patients. To date, little prospective data is available for the optimal sequencing of these agents in melanoma patients. In this retrospective, single-center, real-world analysis, we identified 135 patients with BRAF-mutated, metastatic melanoma who received consecutive treatment with TT followed by CPI, or vice versa, as first and second-line therapy, respectively. We collected data on clinical-pathological factors, treatment duration, best overall response, progression-free survival and overall survival (OS). Our data revealed that front-line treatment with CPI, followed by TT, showed a non-significant trend towards better OS compared to front-line TT (median OS: 35.0 vs. 18.0 months, p = 0.070). This association was confirmed in a subgroup of patients without systemic pre-treatments (median OS: 41.0 vs. 14.0 months, p = 0.02). Further, we observed significantly better objective response rates to second-line treatments for patients receiving front-line CPI (18.4 vs. 37.8%, p = 0.024). Last, our results indicated that rapid disease progression was less common in patients treated with front-line CPI (27.6% vs. 16.2%) and that subsequent treatment with TT resulted in favorable survival outcomes. Our real-world data indicate that sequential treatment with front-line CPI is associated with favorable tumor control and overall survival in a subgroup of previously untreated BRAF-mutant metastatic melanoma patients.
High-Throughput Profiling of Colorectal Cancer Liver Metastases Reveals Intra- and Inter-Patient Heterogeneity in the EGFR and WNT Pathways Associated with Clinical Outcome
Authors: Menck Kerstin , Wlochowitz Darius , Wachter Astrid , Conradi Lena-Christin , Wolff Alexander , Scheel Andreas , Korf Ulrike , Wiemann Stefan , Schildhaus Hans-Ulrich , Bohnenberger Hanibal , Wingender Edgar , Pukrop Tobias , Homayounfar Kia , Beißbarth Tim , Bleckmann Annalen
Published: April 2022

Seventy percent of patients with colorectal cancer develop liver metastases (CRLM), which are a decisive factor in cancer progression. Therapy outcome is largely influenced by tumor heterogeneity, but the intra- and inter-patient heterogeneity of CRLM has been poorly studied. In particular, the contribution of the WNT and EGFR pathways, which are both frequently deregulated in colorectal cancer, has not yet been addressed in this context. To this end, we comprehensively characterized normal liver tissue and eight CRLM from two patients by standardized histopathological, molecular, and proteomic subtyping. Suitable fresh-frozen tissue samples were profiled by transcriptome sequencing (RNA-Seq) and proteomic profiling with reverse phase protein arrays (RPPA) combined with bioinformatic analyses to assess tumor heterogeneity and identify WNT- and EGFR-related master regulators and metastatic effectors. A standardized data analysis pipeline for integrating RNA-Seq with clinical, proteomic, and genetic data was established. Dimensionality reduction of the transcriptome data revealed a distinct signature for CRLM differing from normal liver tissue and indicated a high degree of tumor heterogeneity. WNT and EGFR signaling were highly active in CRLM and the genes of both pathways were heterogeneously expressed between the two patients as well as between the synchronous metastases of a single patient. An analysis of the master regulators and metastatic effectors implicated in the regulation of these genes revealed a set of four genes (SFN, IGF2BP1, STAT1, PIK3CG) that were differentially expressed in CRLM and were associated with clinical outcome in a large cohort of colorectal cancer patients as well as CRLM samples. In conclusion, high-throughput profiling enabled us to define a CRLM-specific signature and revealed the genes of the WNT and EGFR pathways associated with inter- and intra-patient heterogeneity, which were validated as prognostic biomarkers in CRC primary tumors as well as liver metastases.
Cancers and COVID-19 Risk: A Mendelian Randomization Study
Authors: Li Zengbin , Wei Yudong , Zhu Guixian , Wang Mengjie , Zhang Lei
Published: April 2022

Observational studies have shown increased COVID-19 risk among cancer patients, but the causality has not been proven yet. Mendelian randomization analysis can use the genetic variants, independently of confounders, to obtain causal estimates which are considerably less confounded. We aimed to investigate the causal associations of cancers with COVID-19 outcomes using the MR analysis. The inverse-variance weighted (IVW) method was employed as the primary analysis. Sensitivity analyses and multivariable MR analyses were conducted. Notably, IVW analysis of univariable MR revealed that overall cancer and twelve site-specific cancers had no causal association with COVID-19 severity, hospitalization or susceptibility. The corresponding p-values for the casual associations were all statistically insignificant: overall cancer (p = 0.34; p = 0.42; p = 0.69), lung cancer (p = 0.60; p = 0.37; p = 0.96), breast cancer (p = 0.43; p = 0.74; p = 0.43), endometrial cancer (p = 0.79; p = 0.24; p = 0.83), prostate cancer (p = 0.54; p = 0.17; p = 0.58), thyroid cancer (p = 0.70; p = 0.80; p = 0.28), ovarian cancer (p = 0.62; p = 0.96; p = 0.93), melanoma (p = 0.79; p = 0.45; p = 0.82), small bowel cancer (p = 0.09; p = 0.08; p = 0.19), colorectal cancer (p = 0.85; p = 0.79; p = 0.30), oropharyngeal cancer (p = 0.31; not applicable, NA; p = 0.80), lymphoma (p = 0.51; NA; p = 0.37) and cervical cancer (p = 0.25; p = 0.32; p = 0.68). Sensitivity analyses and multivariable MR analyses yielded similar results. In conclusion, cancers might have no causal effect on increasing COVID-19 risk. Further large-scale population studies are needed to validate our findings.
High Dose Local Photon Irradiation Is Crucial in Anti-CTLA-4 Antibody Therapy to Enhance the Abscopal Response in a Murine Pancreatic Carcinoma Model
Authors: Yamamoto Junya , Takahashi Yutaka , Minami Kazumasa , Tamari Keisuke , Katsuki Shohei , Takenaka Wataru , Tatekawa Shotaro , Hayashi Kazuhiko , Seo Yuji , Isohashi Fumiaki , Ogawa Kazuhiko , Koizumi Masahiko
Published: April 2022

Pancreatic cancer is an extremely treatment-resistant neoplasm to chemotherapy and immunotherapy. The combination of photon beam irradiation and anti-CTLA-4 antibody (C4) for the anti-tumor effect enhancement at local and distant tumors (abscopal tumors) was investigated using the pancreatic ductal adenocarcinoma (PDAC) mouse model. Pan02 cells were bilaterally inoculated to both legs of C57BL/6 mice. High dose photon beams in a hypofractionation or a single fraction were delivered to the tumors on one leg. Monotherapy with C4 via i.p. was not effective for PDAC. The high dose irradiation to the local tumors produced significant shrinkage of irradiated tumors but did not induce the abscopal responses. In contrast, the combination therapy of high dose photon beam irradiation in both hypofractionation and a single fraction with C4 enhanced the anti-tumor effect for abscopal tumors with significantly prolonged overall survival. The flow cytometric analysis revealed that the combination therapy dramatically decreased the regulatory T cell (Treg) proportion while increasing the cytotoxic T lymphocytes in both local and abscopal tumors. These results suggest that high dose photon beam irradiation plays an important role in C4 therapy to enhance the abscopal response with immune microenvironment changes in PDAC, regardless of the fractionation in radiation therapy.
Aspergillus flavus-Mediated Green Synthesis of Silver Nanoparticles and Evaluation of Their Antibacterial, Anti-Candida, Acaricides, and Photocatalytic Activities
Authors: Fouda Amr , Awad Mohamed A. , AL-Faifi Zarraq E. , Gad Mohammed E. , Al-Khalaf Areej A. , Yahya Reham , Hamza Mohammed F.
Published: April 2022

Aspergillus flavus F5 was used to reduce AgNO3 to form silver nanoparticles (Ag-NPs) that were monitored by a color change from colorless to yellowish-brown. The characterizations were achieved by UV-Vis spectroscopy, FT-IR, TEM, SEM-EDX, and XRD. Data showed that there was a successful formation of crystalline, spherical shape Ag-NPs with a particle average size of 12.5 ± 5.1 nm. The FT-IR clarified the role of various functional groups in the reducing/capping process. EDX-SEM revealed that the main component of the as-formed sample was set to be mainly Ag with a weight percentage of 46.1%. The synthesized Ag-NPs exhibit antibacterial and anti-Candida activity against Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, Escherichia coli, Candida albicans, C. glabrata, C. tropicalis, and C. parapsilosis, with inhibition zones ranging between 9.3 ± 0.5 to 20.8 ± 0.3 nm based on concentrations used and MIC values between 6.25 to 25 ppm. The mortality percentages of Tyrophagus putrescentiae mite species due to the mixing of their diet with different Ag-NPs concentrations of 0.5, 1.0, and 1.5 mg were 55.7 ± 2.1, 73.3 ± 1.5, and 87.4 ± 1.6% respectively after 20 days post-treatment. The catalytic activity of Ag-NPs to degrade methylene blue (MB) was investigated in the presence and absence of light irradiation. Data showed that a high photocatalytic degradation of MB compared with dark conditions at various times and concentrations. At a concentration of 70 mg/30 mL after 200 min., the dye removal percentages were 86.4 ± 0.4% in the presence of light irradiation versus 66.5 ± 1.1% in dark conditions.
Immunogenic Cell Death, DAMPs and Prothymosin α as a Putative Anticancer Immune Response Biomarker
Authors: Birmpilis Anastasios I. , Paschalis Antonios , Mourkakis Apostolis , Christodoulou Panayiota , Kostopoulos Ioannis V. , Antimissari Elina , Terzoudi Georgia , Georgakilas Alexandros G. , Armpilia Christina , Papageorgis Panagiotis , Kastritis Efstathios , Terpos Evangelos , Dimopoulos Meletios A. , Kalbacher Hubert , Livaniou Evangelia , Christodoulou Maria-Ioanna , Tsitsilonis Ourania E.
Published: April 2022

The new and increasingly studied concept of immunogenic cell death (ICD) revealed a previously unknown perspective of the various regulated cell death (RCD) modalities, elucidating their immunogenic properties and rendering obsolete the notion that immune stimulation is solely the outcome of necrosis. A distinct characteristic of ICD is the release of danger-associated molecular patterns (DAMPs) by dying and/or dead cells. Thus, several members of the DAMP family, such as the well-characterized heat shock proteins (HSPs) HSP70 and HSP90, the high-mobility group box 1 protein and calreticulin, and the thymic polypeptide prothymosin α (proTα) and its immunoreactive fragment proTα(100–109), are being studied as potential diagnostic tools and/or possible therapeutic agents. Here, we present the basic aspects and mechanisms of both ICD and other immunogenic RCD forms; denote the role of DAMPs in ICD; and further exploit the relevance of human proTα and proTα(100–109) in ICD, highlighting their possible clinical applications. Furthermore, we present the preliminary results of our in vitro studies, which show a direct correlation between the concentration of proTα/proTα(100–109) and the levels of cancer cell apoptosis, induced by anticancer agents and γ-radiation.
Association between Information Dissemination and Compliance with Preventive Measures during the Coronavirus Disease Pandemic in Hong Kong Working Population: Cross-Sectional Survey
Authors: Ng Clement Cheuk-Wai , Wong Eliza Lai-Yi , Ho Kin-Fai , Cheung Annie Wai-Ling , Wong Samuel Yeung-Shan , Yeoh Eng-Kiong
Published: April 2022

Background: To fight the Coronavirus disease (COVID-19) pandemic, it is important for the population to keep abreast of COVID-19 updates and comply with the suggested preventive measures. Understanding the influence of popular dissemination channels under the surge of an ‘infodemic’ is crucial, as the population may receive conflicting information from various sources. Objective: This study aimed to examine the association between information source usage and COVID-19-preventive measures compliance. Methods: An online cross-sectional study was conducted in February 2020. Four COVID-19-preventive strategies, including ‘hand hygiene’, ‘mask wearing’, ‘household hygiene’, and ‘social distancing’, were studied with respect to their usage from three common health information sources and three dissemination channels. Logistic regressions were modelled to study the odds ratio of the preventive behavior compliance in terms of information source usage. Results: A total of 1048 respondents completed the survey and the sample demonstrated high compliance in hand hygiene (81.4%) and mask wearing (93.5%), but lower compliance in household hygiene (22.4%) and social distancing (65.7%). Females and chronic diseases patients were found more likely to adopt COVID-19-preventive measures. Participants recorded highest usage in social media (80.1%) among information sources and respondents with frequent social media use had improved compliances in the preventive behaviors studied. Conclusions: The study presented evidence to demonstrate the effectiveness of social media in disseminating information related to complying COVID-19-preventive behaviors. The impact of social media in spreading COVID-19 information should be recognized, despite the concerns regarding misinformation. With disciplined use, social media may help to halt the spread of COVID-19 and other communicable diseases by encouraging community participation.
Effect of Plasma Electrolytic Oxidation on the Short-Term Corrosion Behaviour of AZ91 Magnesium Alloy in Aggressive Chloride Environment
Authors: Štrbák Milan , Kajánek Daniel , Knap Vidžaja , Florková Zuzana , Pastorková Jana , Hadzima Branislav , Goraus Matej
Published: April 2022

In order to increase the corrosion resistance of magnesium alloy AZ91 in corrosion environments containing chlorides, the alloy surface has been modified by plasma electrolytic oxidation (PEO). The chemical composition of electrolyte in the PEO process consisted of 12 g/L Na3PO4·12 H2O and 1 g/L KOH, and a direct current was applied to the sample. The corrosion resistance of PEO coating and as-cast AZ91 (sample without PEO coating) was assessed using two different electrochemical methods: electrochemical impedance spectroscopy (EIS) and potentiodynamic polarisation (PDP) in 0.1 M NaCl at laboratory temperature. In addition to the electrochemical methods, the morphology of the oxidic coating was observed in the cross-sectional and top surface view by using the SEM technique. For better determination of the microstructure and PEO coating, chemical composition EDX analysis was used. The results of the experiments show that the formation of the PEO coating on AZ91 alloy has a more positive effect on the corrosion resistance in 0.1 M NaCl based on electrochemical methods than in the case of the formed coating on AZ31 alloy from the previous study. Based on electrochemical measurements in the selected environment, the formation of PEO coating on AZ91 was accompanied by a significant increase in polarisation resistance after short-term exposure compared to the as-cast surface. The EIS results showed a 73 times higher Rp value for PEO coated AZ91 when compared to the as-cast AZ91. Correspondingly, a 27 times lower icorr value was observed for PEO coated AZ91 than in the case of substrate AZ91 in 0.1 M NaCl. At the same time, the typically porous and inhomogeneous structure of the formed PEO coating on the magnesium alloy AZ91 was demonstrated.
Application Prospects of Blockchain Technology to Support the Development of Interport Communities
Authors: Serra Patrizia , Fancello Gianfranco , Tonelli Roberto , Marchesi Lodovica
Published: April 2022

A key aspect for the efficiency and security of maritime transport is linked to the associated information flows. The optimal management of maritime transport requires the sharing of data in real-time between the various participating organizations. Moreover, as supply chains become increasingly integrated, the connectivity of stakeholders must be ensured not only within the single port but also between ports. Blockchain could offer interesting opportunities in this regard and is believed to have a huge impact on the future of the digitization of the port and maritime industry. This document analyzes the state of art and practice of blockchain applications in the maritime industry and explores the application prospects and practical implications of blockchain for building an interport community. The paper uses SWOT analysis to address several research questions concerning the practical impacts and barriers related to the implementation of blockchain technology in port communities and develops a Proof of Concept (PoC) to concretely show how blockchain technology can be applied to roll-on roll-off transport and interport communities in real environments. In this regard, this study intends to contribute to the sector literature by providing a detailed framework that describes how to proceed to choose the correct blockchain scheme and implement the various management and operational aspects of an interport community by benefiting from the blockchain.
Enrichment-Free Single-Cell Detection and Morphogenomic Profiling of Myeloma Patient Samples to Delineate Circulating Rare Plasma Cell Clones
Authors: Ndacayisaba Libere J. , Rappard Kate E. , Shishido Stephanie N. , Ruiz Velasco Carmen , Matsumoto Nicholas , Navarez Rafael , Tang Guilin , Lin Pei , Setayesh Sonia M. , Naghdloo Amin , Hsu Ching-Ju , Maney Carlisle , Symer David , Bethel Kelly , Kelly Kevin , Merchant Akil , Orlowski Robert , Hicks James , Mason Jeremy , Manasanch Elisabeth E. , Kuhn Peter
Published: April 2022

Multiple myeloma is an incurable malignancy that initiates from a bone marrow resident clonal plasma cell and acquires successive mutational changes and genomic alterations, eventually resulting in tumor burden accumulation and end-organ damage. It has been recently recognized that myeloma secondary genomic events result in extensive sub-clonal heterogeneity both in localized bone marrow areas and circulating peripheral blood plasma cells. Rare genomic subclones, including myeloma initiating cells, could be the drivers of disease progression and recurrence. Additionally, evaluation of rare myeloma cells in blood for disease monitoring has numerous advantages over invasive bone marrow biopsies. To this end, an unbiased method for detecting rare cells and delineating their genomic makeup enables disease detection and monitoring in conditions with low abundant cancer cells. In this study, we applied an enrichment-free four-plex (CD138, CD56, CD45, DAPI) immunofluorescence assay and single-cell DNA sequencing for morphogenomic characterization of plasma cells to detect and delineate common and rare plasma cells and discriminate between normal and malignant plasma cells in paired blood and bone marrow aspirates from five patients with newly diagnosed myeloma (N = 4) and monoclonal gammopathy of undetermined significance (n = 1). Morphological analysis confirms CD138+CD56+ cells in the peripheral blood carry genomic alterations that are clonally identical to those in the bone marrow. A subset of altered CD138+CD56- cells are also found in the peripheral blood consistent with the known variability in CD56 expression as a marker of plasma cell malignancy. Bone marrow tumor clinical cytogenetics is highly correlated with the single-cell copy number alterations of the liquid biopsy rare cells. A subset of rare cells harbors genetic alterations not detected by standard clinical diagnostic methods of random localized bone marrow biopsies. This enrichment-free morphogenomic approach detects and characterizes rare cell populations derived from the liquid biopsies that are consistent with clinical diagnosis and have the potential to extend our understanding of subclonality at the single-cell level in this disease. Assay validation in larger patient cohorts has the potential to offer liquid biopsy for disease monitoring with similar or improved disease detection as traditional blind bone marrow biopsies.
Accessing the Accuracy of Citizen Science Data Based on iNaturalist Data
Authors: Koo Kyo-Soung , Oh Jeong-Min , Park Soo-Jeong , Im Jong-Yoon
Published: April 2022

The number of science projects with citizen (volunteer) participants is increasing nowadays. Despite some advantages citizen science have which directly influence science fields, the largest weakness of citizen science is the issue of the reliability of data collected by non-specialists. In this study, we assess the reliability of data collected by citizens and identify the factors that impede the credibility. We selected two species, Pelophylax chosenicus and Dryophytes suweonensis, which have specific habitat, breeding and brumation positions. The location data for both species were collected by the global citizen engagement project “iNaturalist”. As a result, 89.3% for P. chosenicus and 37.1% for D. suweonensis of the location data were estimated to be erroneous. No difference was observed in the data accuracy between experts and citizens for D. suweonensis because the mating call is the main classification key for the species. On the other hand, a significant difference was confirmed in the case of P. chosenicus, whose external characteristics are its classification key. Our study shows that efforts to improve reliability, including appropriate species selection and survey methods, are necessary to use citizen participation data in science research.
Advancing Mitochondrial Metagenomics: A New Assembly Strategy and Validating the Power of Seed-Based Approach
Authors: Du Shiyu , Dong Jie , Godeiro Nerivânia N. , Wu Jun , Zhang Feng
Published: April 2022

Mitochondrial metagenomics (MMG) using Illumina sequencers for mixed-species samples provides a promising tool for evolutionary and ecological studies using mitogenomes. However, the traditional assembly procedure is still computationally intensive and expensive. Here, a novel MMG pipeline was applied to different DNA extractions, one per species, and their sequence as a mixed sample for rapid mitogenome assembly is presented. Our method integrated a faster and more accurate read mapper for filtering non-mitochondrial reads. A seed-and-extend assembler for species-specific mitogenomes that detects ‘noisy species/sequences’ was also assessed. The MMG pipeline for each dataset was completed in a few hours on desktop PCs, maintaining high accuracy and completeness (COI divergence >10%), except for some very closely related taxa. Particularly for closely related species, the exclusion of ‘noisy reads’ (including chimera of non-targeted species) improved the target assembly. In addition, we observed that short barcodes used as references had almost identical detection power compared with mitogenomes but required greater sequencing depth. We tested our MMG pipeline on two real and one simulated dataset to validate its high efficiency in mixed-species sample mitogenome assembly.
Dynamic Shifts in the Root Microbiota of Cultivated Paphiopedilum armeniacum during Different Stages of Growth
Authors: Cao Xiaolu , Wang Xiaojing , Wang Tao , Chen Yan , Yao Na
Published: April 2022

Paphiopedilum armeniacum S. C. Chen et F. Y. Liu is an endangered lady’s slipper orchid species with high horticultural value. As observed for other orchids, mycorrhizal fungi and endophytic bacteria play important roles in the growth and development of P. armeniacum. In the present study, the community structure dynamics across three growth and development stages of cultivated P. armeniacum were investigated. The potential interactions between Tulasnellaceae fungi and core bacterial genera on one hand and the stability of the presumed mycorrhizal fungi communities on the other were analyzed in three growth stages of P. armeniacum to enhance our understanding of endophytic microbial community structure dynamics in the roots at different development stages. Based on sequencing, 3 and 16 phyla and 59 and 269 genera were identified in the fungal and bacterial communities, respectively. The predominant fungi and bacteria were Basidiomycota (62.90%) and Proteobacteria (43.98%), which exhibited changes in abundance and diversity depending on the growth stage of P. armeniacum. Assessment of the entire microbial communities from different growth stages showed that the seedling stage had the highest richness and diversity. The microbial communities recruited by P. armeniacum at the seedling stage were different from those recruited at the vegetative and reproductive growth stages, and the microbial communities recruited in the latter two stages overlapped. Tulasnellaceae were the only dominant fungal symbionts during P. armeniacum growth. Brevibacillus, Mycobacterium, and Sphingomonas, the three core genera, showed significant interactions with the main OTUs of Tulasnellaceae. Putative mycorrhizal fungi in P. armeniacum were relatively stable across different growth environments, and the core mycorrhizal fungi were uncultured Tulasnellaceae (OTU1). This could facilitate the ex situ conservation and commercial development of the endangered orchid.
Genetic and Population Structure of Croatian Local Donkey Breeds
Authors: Ivanković Ante , Bittante Giovanni , Šubara Gordan , Šuran Edmondo , Ivkić Zdenko , Pećina Mateja , Konjačić Miljenko , Kos Ivica , Kelava Ugarković Nikolina , Ramljak Jelena
Published: April 2022

The two native Croatian donkey breeds (Littoral-Dinaric donkey and Istrian donkey) were marginalized in the second half of the 20th century and were on the verge of biological extinction. The aim of this study was to analyze the demographic and genetic status of two donkey breeds, two decades after the start of protection by analyzing their pedigrees and genetic structure. The average generation interval was higher for the Istrian donkey (7.73) than for the Littoral-Dinaric donkey (7.27). The rate of the effective number of founders compared with the effective number of ancestors in the Littoral-Dinaric donkey (1.03; 325/316) and in the Istrian donkey (1.08; 70/65) revealed no evidence of a genetic bottleneck. The inbreeding coefficient (F) and the average relatedness coefficient (AR) was lower in the Littoral-Dinaric donkey population (0.99%; 0.13%) than in the Istrian donkey population (1.77%; 1.10%). Genetic microsatellite analysis showed relatively high genetic diversity in Littoral-Dinaric donkey and Istrian donkey breeds, expressed by mean allele number (5.92; 5.85) and expected heterozygosity (0.650; 0.653). Genetic differentiation between the Littoral-Dinaric donkey and the Istrian donkey has not significantly increased in the last two decades (FST = 0.028). Genetic analysis also showed no evidence of high inbreeding or genetic bottleneck in both breeds. A total of 11 haplotypes including 28 polymorphic sites were found in 30 samples. Analysis of mtDNA has shown that the Littoral-Dinaric donkey and Istrian donkey breeds belong to the Equus asinus africanus group. The study confirms the need to use different analytical approaches to get a regular and complete insight into the situation and trends within and between breeds, so that the existing diversity can be fully preserved.
Evaluating the Cisplatin Dose Dependence of Testicular Dysfunction Using Creatine Chemical Exchange Saturation Transfer Imaging
Authors: Sawaya Reika , Kuribayashi Sohei , Ueda Junpei , Saito Shigeyoshi
Published: April 2022

Chemical exchange saturation transfer (CEST) imaging is a non-invasive molecular imaging technique for indirectly measuring low-concentration endogenous metabolites. Conventional CEST has low specificity, owing to the effects of spillover, magnetization transfer (MT), and T1 relaxation, thus necessitating an inverse Z-spectrum analysis. We aimed to investigate the usefulness of inverse Z-spectrum analysis in creatine (Cr)-CEST in mice, by conducting preclinical 7T-magnetic resonance imaging (MRI) and comparing the conventional analysis metric magnetization transfer ratio (MTRconv) with the novel metric apparent exchange-dependent relaxation (AREX). We performed Cr-CEST imaging using 7T-MRI on mouse testes, using C57BL/6 mice as the control and a cisplatin-treated model. We prepared different doses of cisplatin to observe its dose dependence effect on testicular function. CEST imaging was obtained using an MT pulse with varying saturation frequencies, ranging from −4.8 ppm to +4.8 ppm. The application of control mouse testes improved the specificity of the CEST effect and image contrast between the testes and testicular epithelium. The cisplatin-treated model revealed impaired testicular function, and the Cr-CEST imaging displayed decreased Cr levels in the testes. There was a significant difference between the low- and high-dose models. The MTR values of Cr-CEST reflected the cisplatin dose dependence of testicular dysfunction.
Basic Considerations and Conceptual Design of a VSTOL Vehicle for Urban Transportation
Authors: Piancastelli Luca , Sali Merve , Leon-Cardenas Christian
Published: April 2022

On-demand air transport is an air-taxi service concept that should ideally use small, autonomous, Vertical Short Takeoff and Landing (VSTOL), “green”, battery-powered electric aircraft (eVSTOL). In addition, these aircraft should be competitive with modern helicopters, which are exceptionally reliable machines capable of the same task. For certification and economic purposes, mobile tilting parts should be avoided. The concept introduced in this paper simplifies the aircraft and makes it economical to build, certify and maintain. Four contrarotating propellers with eight electric motors are installed. During cruise, only two of the eight rotors available are not feathered and active. In the first step, a commercial, certified, jet-fueled APU and an available back-up battery are used. A second solution uses a CNG APU and the same back-up battery. Finally, the third solution has a high-density dual battery that is currently not available. A conceptual design is shown in this paper.
Review of the Dynamics of Atomic and Molecular Systems of Higher than Geometric Symmetry—Part I: One-Electron Rydberg Quasimolecules
Authors: Kryukov Nikolay , Oks Eugene
Published: April 2022

The review covers the dynamics of different kinds of one electron Rydberg quasimolecules in various environments, such as being subjected to electric and/or magnetic fields or to a plasma environment. The higher than geometrical symmetry of these systems is due to the existence of an additional conserved quantity: the projection of the supergeneralized Runge–Lenz vector on the internuclear axis. The review emphasizes the fundamental and practical importance of the results concerning the dynamics of these systems.
Return Probability of Quantum and Correlated Random Walks
Authors: Kiumi Chusei , Konno Norio , Tamura Shunya
Published: April 2022

The analysis of the return probability is one of the most essential and fundamental topics in the study of classical random walks. In this paper, we study the return probability of quantum and correlated random walks in the one-dimensional integer lattice by the path counting method. We show that the return probability of both quantum and correlated random walks can be expressed in terms of the Legendre polynomial. Moreover, the generating function of the return probability can be written in terms of elliptic integrals of the first and second kinds for the quantum walk.
The Impact of Partial Balance of Imbalanced Dataset on Classification Performance
Authors: Li Qing , Zhao Chang , He Xintai , Chen Kun , Wang Runze
Published: April 2022

The imbalance of network data seriously affects the classification performance of algorithms. Most studies have only used a rough description of data imbalance with less exploration of the specific factors affecting classification performance, which has resulted in difficulty putting forward targeted solutions. In this paper, we find that the impact of medium categories on classification performance cannot be ignored, and therefore propose the concept of partial balance, consisting of Class Number of Partial Balance (β) and Balance Degree of Partial Samples (μ). Combined with Global Slope (α), a parameterized model is established to describe the difference of imbalanced datasets. Experiments are performed on the Moore Dataset and CICIDS 2017 Dataset. The experiment’s results on Random Forest, Decision Tree and Deep Neural Network show increasing α is a conducive step in the performance improvement of minority classes and overall classes. When β of dominant categories increases, that of inferior classes decreases, which results in a decrease in the average performance of minority classes. The lower μ is, the closer the sample size of medium classes is to the minority classes, and the better the average performance is. Based on the conclusions, we propose and verify some basic strategies by various classical algorithms.
Global Formulation of the Synthetic Storm Technique Oriented to Satellite Link–Budget Design
Authors: Matricciani Emilio , Riva Carlo
Published: April 2022

We have updated the global Synthetic Storm Technique (referred to as the global SST) by reformulating it according to a larger database of rain rate time series collected in several sites in different climatic regions. For each site, the average annual probability distribution of rain attenuation obtained with the global SST, PSST,gloA, in a slant path, was compared with that given by the full SST, PSSTA, which we have considered as experimental data. The test was performed for frequency ranging from 10 to 100 GHz, for elevation angle θ ranging from 20° to 60° and for annual probabilities 10% to 0.01%. The global SST tends to underestimate the attenuation by approximately 10% for elevation angle θ≤30° and about 20% for 30°<θ<60° in the probability range 10% to 0.1%, and approximately 15% in the probability range 0.1% to 0.01%. For any probability, the error is zero for θ=90° because at the zenith, the global SST coincides with the full SST.
Influence of As-N Interstitial Complexes on Strain Generated in GaAsN Epilayers Grown by AP-MOVPE
Authors: Ściana Beata , Dawidowski Wojciech , Radziewicz Damian , Jadczak Joanna , López-Escalante Mari Cruz , González de la Cruz Victor , Gabás Mercedes
Published: April 2022

This work presents an investigation of the fully strained GaAsN/GaAs heterostructures obtained by atmospheric pressure metalorganic vapor phase epitaxy, focusing on the analysis of the strain generated in the GaAsN epilayers and its correlation with the formation of split interstitial complexes (N-As)As. We analyzed strained GaAsN epilayers with nitrogen contents and thicknesses varying from 0.93 to 1.81% and 65 to 130 nm, respectively. The composition and thickness were determined by high resolution X-ray diffraction, and the strain was determined by Raman spectroscopy, while the N-bonding configurations were determined by X-ray photoelectron spectroscopy. We found that the strain generated in the GaAsN epilayers is mainly caused by a lattice mismatch with the GaAs substrate. This macroscopic strain is independent of the amount of (N-As)As interstitial defects, while the local strain, induced by an alloying effect, tends to decrease with an increasing ratio of (N-As)As interstitial defects to substitutional nitrogen atoms incorporated into an arsenic sublattice—NAs. Here, we show experimentally, for the first time, a correlation between the strain in the GaAsN epilayers, caused by an alloying effect determined by Raman spectroscopy, and the (N-As)As/NAs ratio estimated by the XPS method. We found out that the (N-As)As interstitials compensate the local strain resulting from the presence of N in the GaAs matrix, if their amount does not exceed ~65% of the substitutional introduced nitrogen NAs.