bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of Modified Glucosamine on the Chondrogenic Potential of Circulating Stem Cells under Experimental Inflammation
Authors: Gasparella Marco , Cenzi Carola , Piccione Monica , Madia Valentina Noemi , Di Santo Roberto , Tudino Valeria , Artico Marco , Taurone Samanta , De Ponte Chiara , Costi Roberta , Di Liddo Rosa
Published: June 2023

Glucosamine (GlcN) is a glycosaminoglycan (GAGs) constituent in connective tissues. It is naturally produced by our body or consumed from diets. In the last decade, in vitro and in vivo trials have demonstrated that the administration of GlcN or its derivates has a protective effect on cartilage when the balance between catabolic and anabolic processes is disrupted and cells are no longer able to fully compensate for the loss of collagen and proteoglycans. To date, these benefits are still controversial because the mechanism of action of GlcN is not yet well clarified. In this study, we have characterized the biological activities of an amino acid (AA) derivate of GlcN, called DCF001, in the growth and chondrogenic induction of circulating multipotent stem cells (CMCs) after priming with tumor necrosis factor-alpha (TNFα), a pleiotropic cytokine commonly expressed in chronic inflammatory joint diseases. In the present work, stem cells were isolated from the human peripheral blood of healthy donors. After priming with TNFα (10 ng/mL) for 3 h, cultures were treated for 24 h with DCF001 (1 μg/mL) dissolved in a proliferative (PM) or chondrogenic (CM) medium. Cell proliferation was analyzed using a Corning® Cell Counter and trypan blue exclusion technique. To evaluate the potentialities of DCF001 in counteracting the inflammatory response to TNFα, we measured the amount of extracellular ATP (eATP) and the expression of adenosine-generating enzymes CD39/CD73, TNFα receptors, and NF-κB inhibitor IκBα using flow cytometry. Finally, total RNA was extracted to perform a gene expression study of some chondrogenic differentiation markers (COL2A1, RUNX2, and MMP13). Our analysis has shed light on the ability of DCF001 to (a) regulate the expression of CD39, CD73, and TNF receptors; (b) modulate eATP under differentiative induction; (c) enhance the inhibitory activity of IκBα, reducing its phosphorylation after TNFα stimulation; and (d) preserve the chondrogenic potentialities of stem cells. Although preliminary, these results suggest that DCF001 could be a valuable supplement for ameliorating the outcome of cartilage repair interventions, enhancing the efficacy of endogenous stem cells under inflammatory stimuli.
COX5A Alleviates Doxorubicin-Induced Cardiotoxicity by Suppressing Oxidative Stress, Mitochondrial Dysfunction and Cardiomyocyte Apoptosis
Authors: Zhang Peipei , Lu Hao , Wu Yuan , Lu Danbo , Li Chenguang , Yang Xiangdong , Chen Zhangwei , Qian Juying , Ge Junbo
Published: June 2023

Doxorubicin (DOX) as a chemotherapeutic agent can cause mitochondrial dysfunction and heart failure. COX5A has been described as an important regulator of mitochondrial energy metabolism. We investigate the roles of COX5A in DOX-induced cardiomyopathy and explore the underlying mechanisms. C57BL/6J mice and H9c2 cardiomyoblasts were treated with DOX, and the COX5A expression was assessed. An adeno-associated virus serum type 9 (AAV9) and lenti-virus system were used to upregulate COX5A expression. Echocardiographic parameters, morphological and histological analyses, transmission electron microscope and immunofluorescence assays were used to assess cardiac and mitochondrial function. In a human study, we found that cardiac COX5A expression was dramatically decreased in patients with end-stage dilated cardiomyopathy (DCM) compared to the control group. COX5A was significantly downregulated following DOX stimulation in the heart of mice and H9c2 cells. Reduced cardiac function, decreased myocardium glucose uptake, mitochondrial morphology disturbance, reduced activity of mitochondrial cytochrome c oxidase (COX) and lowered ATP content were detected after DOX stimulation in mice, which could be significantly improved by overexpression of COX5A. Overexpression of COX5A effectively protected against DOX-induced oxidative stress, mitochondrial dysfunction and cardiomyocyte apoptosis in vivo and in vitro. Mechanistically, the phosphorylation of Akt (Thr308) and Akt (Ser473) were also decreased following DOX treatment, which could be reserved by the upregulation of COX5A. Furthermore, PI3K inhibitors abrogated the protection effects of COX5A against DOX-induced cardiotoxicity in H9c2 cells. Thus, we identified that PI3K/Akt signaling was responsible for the COX5A-mediated protective role in DOX-induced cardiomyopathy. These results demonstrated the protective effect of COX5A in mitochondrial dysfunction, oxidative stress, and cardiomyocyte apoptosis, providing a potential therapeutic target in DOX-induced cardiomyopathy.
Genetic and Epigenetic Mechanisms of Longevity in Forest Trees
Authors: Batalova Anastasia Y. , Krutovsky Konstantin V.
Published: June 2023

Trees are unique in terms of development, sustainability and longevity. Some species have a record lifespan in the living world, reaching several millennia. The aim of this review is to summarize the available data on the genetic and epigenetic mechanisms of longevity in forest trees. In this review, we have focused on the genetic aspects of longevity of a few well-studied forest tree species, such as Quercus robur, Ginkgo biloba, Ficus benghalensis and F. religiosa, Populus, Welwitschia and Dracaena, as well as on interspecific genetic traits associated with plant longevity. A key trait associated with plant longevity is the enhanced immune defense, with the increase in gene families such as RLK, RLP and NLR in Quercus robur, the expansion of the CC-NBS-LRR disease resistance families in Ficus species and the steady expression of R-genes in Ginkgo biloba. A high copy number ratio of the PARP1 family genes involved in DNA repair and defense response was found in Pseudotsuga menziesii, Pinus sylvestris and Malus domestica. An increase in the number of copies of the epigenetic regulators BRU1/TSK/MGO3 (maintenance of meristems and genome integrity) and SDE3 (antiviral protection) was also found in long-lived trees. CHG methylation gradually declines in the DAL 1 gene in Pinus tabuliformis, a conservative age biomarker in conifers, as the age increases. It was shown in Larix kaempferi that grafting, cutting and pruning change the expression of age-related genes and rejuvenate plants. Thus, the main genetic and epigenetic mechanisms of longevity in forest trees were considered, among which there are both general and individual processes.
Microbiota and Glucidic Metabolism: A Link with Multiple Aspects and Perspectives
Authors: Ciarambino Tiziana , Crispino Pietro , Leto Gaetano , Minervini Giovanni , Para Ombretta , Giordano Mauro
Published: June 2023

The global prevalence of overweight and obesity has dramatically increased in the last few decades, with a significant socioeconomic burden. In this narrative review, we include clinical studies aiming to provide the necessary knowledge on the role of the gut microbiota in the development of diabetic pathology and glucose-metabolism-related disorders. In particular, the role of a certain microbial composition of the fermentative type seems to emerge without a specific link to the development in certain subjects of obesity and the chronic inflammation of the adipose tissues, which underlies the pathological development of all the diseases related to glucose metabolism and metabolic syndrome. The gut microbiota plays an important role in glucose tolerance. Conclusion. New knowledge and new information is presented on the development of individualized therapies for patients affected by all the conditions related to reduced glucose tolerance and insulin resistance.
Integrated Transcriptome and Metabolome Analysis Revealed the Causal Agent of Primary Bud Necrosis in ‘Summer Black’ Grape
Authors: Fan Shaogang , Xu Yanshuai , Bai Miao , Luo Feixiong , Yu Jun , Yang Guoshun
Published: June 2023

Primary bud necrosis of grape buds is a physiological disorder that leads to decreased berry yield and has a catastrophic impact on the double cropping system in sub-tropical areas. The pathogenic mechanisms and potential solutions remain unknown. In this study, the progression and irreversibility patterns of primary bud necrosis in ‘Summer Black’ were examined via staining and transmission electron microscopy observation. Primary bud necrosis was initiated at 60 days after bud break and was characterized by plasmolysis, mitochondrial swelling, and severe damage to other organelles. To reveal the underlying regulatory networks, winter buds were collected during primary bud necrosis progression for integrated transcriptome and metabolome analysis. The accumulation of reactive oxygen species and subsequent signaling cascades disrupted the regulation systems for cellular protein quality. ROS cascade reactions were related to mitochondrial stress that can lead to mitochondrial dysfunction, lipid peroxidation causing damage to membrane structure, and endoplasmic reticulum stress leading to misfolded protein aggregates. All these factors ultimately resulted in primary bud necrosis. Visible tissue browning was associated with the oxidation and decreased levels of flavonoids during primary bud necrosis, while the products of polyunsaturated fatty acids and stilbenes exhibited an increasing trend, leading to a shift in carbon flow from flavonoids to stilbene. Increased ethylene may be closely related to primary bud necrosis, while auxin accelerated cell growth and alleviated necrosis by co-chaperone VvP23-regulated redistribution of auxin in meristem cells. Altogether, this study provides important clues for further study on primary bud necrosis.
Identification and Functional Characterization of ZmSCYL2 Involved in Phytosterol Accumulation in Plants
Authors: Zhang Chenchen , Ma Wanlu , Xu Minyan , Li Tao , Han Guomin , Gu Longjiang , Chen Meng , Zhang Mengting , Cheng Beijiu , Zhang Xin
Published: June 2023

Phytosterols are natural active substances widely found in plants and play an important role in hypolipidemia, antioxidants, antitumor, immunomodulation, plant growth, and development. In this study, phytosterols were extracted and identified from the seed embryos of 244 maize inbred lines. Based on this, a genome-wide association study (GWAS) was used to predict the possible candidate genes responsible for phytosterol content; 9 SNPs and 32 candidate genes were detected, and ZmSCYL2 was identified to be associated with phytosterol accumulation. We initially confirmed its functions in transgenic Arabidopsis and found that mutation of ZmSCYL2 resulted in slow plant growth and a significant reduction in sterol content, while overexpression of ZmSCYL2 accelerated plant growth and significantly increased sterol content. These results were further confirmed in transgenic tobacco and suggest that ZmSCYL2 was closely related to plant growth; overexpression of ZmSCYL2 not only facilitated plant growth and development but also promoted the accumulation of phytosterols.
Phoenix dactylifera (Ajwa Dates) Alleviate LPS-Induced Sickness Behaviour in Rats by Attenuating Proinflammatory Cytokines and Oxidative Stress in the Brain
Authors: Shivanandappa Thippeswamy Boreddy , Alotaibi Ghallab , Chinnadhurai Maheswari , Dachani Sudharshan Reddy , Ahmad Mahmad Dabeer , Aldaajanii Khalid Abdullah
Published: June 2023

Traditional medicine claims that various components of the Phoenix dactylifera (date plant) can be used to treat memory loss, fever, inflammation, loss of consciousness, and nerve disorders. The present study aims to evaluate the effectiveness of Phoenix dactylifera fruit extracts (PDF) against rat sickness behaviour caused by lipopolysaccharide (LPS) by assessing behavioural and biochemical parameters. PDF was prepared by extracting dry fruits of P. dactylifera with a methanol:water (4:1, v/v) mixture. The PDF was evaluated for phenolic and flavonoid content and HPLC analysis of quercetin estimation. Adult Wistar rats were treated with LPS, PDF + LPS and dexamethasone + LPS. Water and food intake, behavioural tests such as locomotor activity, tail suspension and forced swim tests were conducted. Furthermore, alanine transaminase (ALT) and aspartate transaminase (AST) were estimated in plasma and malondialdehyde (MDA), reduced glutathione (GSH), nitrite, tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), were estimated in the brain. PDF ameliorated LPS-induced sickness behaviour by reducing MDA, nitrite, IL-6, and TNF-α levels and improving GSH, behavioural alteration, water and food intake in the treated rats. In the plasma of the treated rats, PDF also decreased the levels of ALT and AST. The outcomes demonstrated the efficacy of PDF in reducing the sickness behaviour caused by LPS in rats. The authors believe that this study will provide the groundwork for future research to better understand the underlying mechanisms of action and therapeutic efficacy.
Repurposing Simvastatin in Parkinson’s Disease Model: Protection Is throughout Modulation of the Neuro-Inflammatory Response in the Substantia nigra
Authors: Rubio-Osornio Moisés , León Carmen T. Goméz-De , Montes Sergio , Rubio Carmen , Ríos Camilo , Monroy Antonio , Morales-Montor Jorge
Published: June 2023

Parkinson’s disease is a neurodegenerative disorder characterized by oxidative stress and immune activation in the nigro-striatal pathway. Simvastatin regulates cholesterol metabolism and protects from atherosclerosis disease. Simvastatin-tween 80 was administered 7 days before sterotaxic intrastriatal administration of MPP+ (1-methyl-4-phenylpyridine) in rats. Fluorescent lipidic product formation, dopamine levels, and circling behavior were considered damage markers. Twenty-four hours and six days after, the animal group lesioned with MPP+ showed significant damage in relation to the control group. Animals pretreated with simvastatin significantly reduced the MPP+-induced damage compared to the MPP+ treated group. As apoptosis promotes neuroinflammation and neuronal degeneration in Parkinson’s disease, and since there is not currently a proteomic map of the nigro-striatum of rats and assuming a high homology among the identified proteins in other rat tissues, we based the search for rat protein homologs related to the establishment of inflammation response. We demonstrate that most proteins related to inflammation decreased in the simvastatin-treated rats. Furthermore, differential expression of antioxidant enzymes in striated tissue of rat brains was found in response to simvastatin. These results suggest that simvastatin could prevent striatal MPP+-induced damage and, for the first time, suggest that the molecular mechanisms involved in this have a protective effect.
IL-22 Is Deleterious along with IL-17 in Allergic Asthma but Is Not Detrimental in the Comorbidity Asthma and Acute Pneumonia
Authors: Goulart Amanda , Boko Mèdéton Mahoussi Michaël , Martins Nubia Sabrina , Gembre Ana Flávia , de Oliveira Rômulo Silva , Palma-Albornoz Sandra Patrícia , Bertolini Thais , Ribolla Paulo Eduardo Martins , Ramalho Leandra Naira Zambelli , Fraga-Silva Thais Fernanda de Campos , Bonato Vânia Luiza Deperon
Published: June 2023

There is evidence that IL-22 and IL-17 participate in the pathogenesis of allergic asthma. To investigate the role of IL-22, we used IL-22 deficient mice (IL-22 KO) sensitized and challenged with ovalbumin (OVA) and compared with wild type (WT) animals exposed to OVA. IL-22 KO animals exposed to OVA showed a decreased number and frequency of eosinophils, IL-5 and IL-13 in the airways, reduced mucus production and pulmonary inflammation. In addition, IL-22 KO animals exhibited a decreased percentage and number of lung CD11c+CD11b+ cells and increased apoptosis of eosinophils. Th17 cell transfer generated from IL-22 KO to animals previously sensitized and challenged with OVA caused a reduction in eosinophil frequency and number in the airways compared to animals transferred with Th17 cells generated from WT mice. Therefore, IL-22 is deleterious with concomitant secretion of IL-17. Our findings show a pro-inflammatory role for IL-22, confirmed in a model of allergen-free and allergen-specific immunotherapy. Moreover, during the comorbidity asthma and pneumonia that induces neutrophil inflammation, IL-22 was not detrimental. Our results show that targeting IL-22 would negatively affect the survival of eosinophils, reduce the expansion or migration of CD11c+CD11b+ cells, and negatively regulate allergic asthma.
Overexpression of 9-cis-Epoxycarotenoid Dioxygenase Gene, IbNCED1, Negatively Regulates Plant Height in Transgenic Sweet Potato
Authors: Zhou Yuanyuan , Zhao Chunling , Du Taifeng , Li Aixian , Qin Zhen , Zhang Liming , Dong Shunxu , Wang Qingmei , Hou Fuyun
Published: June 2023

Plant height is one of the key agronomic traits for improving the yield of sweet potato. Phytohormones, especially gibberellins (GAs), are crucial to regulate plant height. The enzyme 9-cis-epoxycarotenoid dioxygenase (NCED) is the key enzyme for abscisic acid (ABA) biosynthesis signalling in higher plants. However, its role in regulating plant height has not been reported to date. Here, we cloned a new NCED gene, IbNCED1, from the sweet potato cultivar Jishu26. This gene encoded the 587-amino acid polypeptide containing an NCED superfamily domain. The expression level of IbNCED1 was highest in the stem and the old tissues in the in vitro-grown and field-grown Jishu26, respectively. The expression of IbNCED1 was induced by ABA and GA3. Overexpression of IbNCED1 promoted the accumulation of ABA and inhibited the content of active GA3 and plant height and affected the expression levels of genes involved in the GA metabolic pathway. Exogenous application of GA3 could rescue the dwarf phenotype. In conclusion, we suggest that IbNCED1 regulates plant height and development by controlling the ABA and GA signalling pathways in transgenic sweet potato.
The Diagnostic Potential of the Human Blood Microbiome: Are We Dreaming or Awake?
Authors: Sciarra Francesca , Franceschini Edoardo , Campolo Federica , Venneri Mary Anna
Published: June 2023

Human blood has historically been considered a sterile environment. Recently, a thriving microbiome dominated by Firmicutes, Actinobacteria, Proteobacteria, and Bacteroidetes phyla was detected in healthy blood. The localization of these microbes is restricted to some blood cell populations, particularly the peripheral blood mononuclear cells and erythrocytes. It was hypothesized that the blood microbiome originates from the skin–oral–gut axis. In addition, many studies have evaluated the potential of blood microbiome dysbiosis as a prognostic marker in cardiovascular diseases, cirrhosis, severe liver fibrosis, severe acute pancreatitis, type 2 diabetes, and chronic kidney diseases. The present review aims to summarize current findings and most recent evidence in the field.
Adduction Manipulation of the Glenohumeral Joint versus Physiotherapy for Atraumatic Rotator Cuff Tears: A Randomized Controlled Trial
Authors: Karasuno Hiroshi , Hamada Junichiro , Yano Yuichiro , Tsutsui Hiroaki , Hagiwara Yoshihiro , Endo Kazuhiro , Saito Takashi
Published: June 2023

Background: Atraumatic rotator cuff tears (ARCTs) are frequently concomitant with adduction restriction of the glenohumeral joint (GHJ). Adduction manipulation (AM) removes the restriction and relieves pain. The present study aimed to investigate the clinical efficacy of AM versus physiotherapy (PT) in ARCTs. Methods: Eighty-eight patients with adduction restriction were allocated to the AM and PT groups (n = 44 per group). The glenohumeral adduction angle (GAA) was calculated using X-rays at the first and last follow-up appointments. We recorded pain severity (visual analog scale, VAS), flexion, abduction, external rotation (ER), internal rotation (IR), and American Shoulder and Elbow Society (ASES) and Constant scores at baseline and at 1-, 3-, 6-, and 12- month follow-ups. Results: Forty-three patients (23 males, average age 71.3 years) in the AM group and 41 (16 males, average age 70.7 years) in the PT group were consequently analyzed. At the 1-month follow-up, VAS, shoulder motion except ER, ASES and Constant scores were much better in the AM group than in the PT group, whereas those in the PT group improved gradually up to 12 months. At the final follow-up, flexion, abduction, and Constant score were significantly better in the AM group than in the PT group. The GAA at the initial and final examinations was −21.6° and −3.2°, respectively, in the AM group, and −21.1° and −14.4°, respectively, in the PT group. Conclusions: The AM procedure, which had better clinical efficacy than PT, is recommended as the first conservative treatment option for ARCTs.
Bony Sacral Volume after Sacro-Iliac Screw Fixation of Pelvic Fractures Is Dependent on Reduction of the Anterior Pelvic Ring
Authors: Baumann Florian , Pagano Stefano , Alt Volker , Freigang Viola
Published: June 2023

Pelvic ring injuries are uncommon but serious injuries. Percutaneous sacro-iliac screw fixation (SSF) is the standard treatment for posterior stabilization of pelvic fractures. Compression forces of the SSF might cause deformity of the sacrum and the pelvic ring. The aim of this radio-volumetric study is to evaluate the morphometry of the sacrum and pelvic ring in SSF for posterior pelvic fractures. (1) Methods: We conducted a radio-volumetric study measuring the bony sacral volume before and after SSF for a pelvic fracture based on a three-dimensional reconstruction of the pre- and postoperative computed tomography scan of 19 patients with a C-type pelvic fracture. In addition to the bony sacral volume, we assessed the pelvic deformity and the load bearing axis. We compared the results of patients without anterior stabilization (Group A) to patients who had additional ORIF of the anterior pelvic ring. (2) Results: Median age of the patients was 41.2 years (±17.8). All patients received percutaneous SSF with partially threaded 7.3 mm screws. The sacral volume decreased from 202.9 to 194.3 cm3 in group A (non-operative treatment anterior, n = 10) and an increase of sacral volume from 229.8 to 250.4 cm3 in group B (anterior ORIF; n = 9). Evaluation of the pelvic deformity also reflected this trend by a decrease of the ipsilateral load-bearing angle in group A (37.0° to 36.4°) and an increase of this angle in group B (36.3 to 39.9°). (3) Conclusions: Bony sacral volume and pelvic deformity after sacro-iliac screw fixation in pelvic fractures depend on treatment of the anterior pelvic ring. Reduction and fixation of the anterior fracture shows an increase of the bony sacral volume and the load bearing angle leading to a closer to normal reconstruction of the pelvic anatomy.
Personalized Respiratory Support in ARDS: A Physiology-to-Bedside Review
Authors: Cutuli Salvatore Lucio , Grieco Domenico Luca , Michi Teresa , Cesarano Melania , Rosà Tommaso , Pintaudi Gabriele , Menga Luca Salvatore , Ruggiero Ersilia , Giammatteo Valentina , Bello Giuseppe , De Pascale Gennaro , Antonelli Massimo
Published: June 2023

Acute respiratory distress syndrome (ARDS) is a leading cause of disability and mortality worldwide, and while no specific etiologic interventions have been shown to improve outcomes, noninvasive and invasive respiratory support strategies are life-saving interventions that allow time for lung recovery. However, the inappropriate management of these strategies, which neglects the unique features of respiratory, lung, and chest wall mechanics may result in disease progression, such as patient self-inflicted lung injury during spontaneous breathing or by ventilator-induced lung injury during invasive mechanical ventilation. ARDS characteristics are highly heterogeneous; therefore, a physiology-based approach is strongly advocated to titrate the delivery and management of respiratory support strategies to match patient characteristics and needs to limit ARDS progression. Several tools have been implemented in clinical practice to aid the clinician in identifying the ARDS sub-phenotypes based on physiological peculiarities (inspiratory effort, respiratory mechanics, and recruitability), thus allowing for the appropriate application of personalized supportive care. In this narrative review, we provide an overview of noninvasive and invasive respiratory support strategies, as well as discuss how identifying ARDS sub-phenotypes in daily practice can help clinicians to deliver personalized respiratory support and potentially improve patient outcomes.
Spatial Visualization Supports Students’ Math: Mechanisms for Spatial Transfer
Authors: Lowrie Tom , Logan Tracy
Published: June 2023

The present study conducted a randomized control trial to assess the efficacy of two spatial intervention programs aimed to improve Grade 4 (N = 287) students’ spatial visualization skills and math performance. The first treatment (N = 98) focused on isolated spatial training that included 40 min of digital spatial training across fourteen weeks. The second treatment (N = 92) embedded spatial visualization skill development into math lessons, along with the digital spatial training that provided practice of the newly acquired skills. A business-as-usual group acted as a control (N = 97). Engagement with the embedded intervention program (i.e., both lessons and digital training) showed large additive effects, highlighting the role of spatial reasoning tools to support the transfer of spatial reasoning to math. The isolated intervention program with the digital spatial training had a transfer effect on math, compared to a business-as-usual control, while spatial reasoning improvements for this group were mixed. The spatial skills targeted in the digital training had a mediation effect on math performance, despite not increasing in the pre–post-test design. The effects of the digital training cohort were moderated by initial spatial skill, with students with lower spatial reasoning making the least gains in math.
Succession of Fungal Community during Outdoor Deterioration of Round Bamboo
Authors: An Xiaojiao , Han Shuaibo , Ren Xin , Sichone John , Fan Zhiwei , Wu Xinxing , Zhang Yan , Wang Hui , Cai Wei , Sun Fangli
Published: June 2023

Bamboo’s mechanical and aesthetic properties are significantly influenced by fungi. However, few studies have been conducted to investigate the structure and dynamics of fungal communities in bamboo during its natural deterioration. In this study, fungal community succession and characteristic variations of round bamboo in roofed and unroofed environments over a period of 13 weeks of deterioration were deciphered using high-throughput sequencing and multiple characterization methods. A total of 459 fungal Operational Taxonomic Units (OTUs) from eight phyla were identified. The fungal community’s richness of roofed bamboo samples showed an increasing trend, whereas that of unroofed bamboo samples presented a declining trend during deterioration. Ascomycota and Basidiomycota were the dominant phyla throughout the deterioration process in two different environments: Basidiomycota was found to be an early colonizer of unroofed bamboo samples. Principal Coordinates Analysis (PCoA) analysis suggested that the deterioration time had a greater impact on fungal community variation compared to the exposure conditions. Redundancy analysis (RDA) further revealed that temperature was a major environmental factor that contributed to the variation in fungal communities. Additionally, the bamboo epidermis presented a descending total amount of cell wall components in both roofed and unroofed conditions. The correlation analysis between the fungal community and relative abundance of three major cell wall components elucidated that Cladosporium was negatively correlated with hemicellulose in roofed samples, whereas they presented a positive correlation with hemicellulose and a negative correlation with lignin in unroofed samples. Furthermore, the contact angle decreased during the deterioration process in the roofed as well as unroofed samples, which could arise from the degradation of lignin. Our findings provide novel insights into the fungal community succession on round bamboo during its natural deterioration and give useful information for round bamboo protection.
Early Complication Analysis of Dynamic Intraligamentary Stabilization versus Anterior Cruciate Ligament Reconstruction
Authors: Endreß Frank , Hörner Reinhard , Hauth Wolfgang , Anders Jens , Biber Roland
Published: June 2023

Purpose: Both dynamic intraligamentary stabilization (DIS) and reconstruction (RECO) are common treatment methods for anterior cruciate ligament (ACL) rupture. We report short term outcomes after DIS (Ligamys, Mathys, Bettlach, Switzerland) and RECO using semitendinosus tendon. We compared postoperative complications, deficits of range-of-motion (ROM), and revision rates between the two treatment options. Methods: A total of 690 patients (437 male, 253 female), after either DIS or RECO, were included. Of these, 147 patients (21%) received DIS and 543 (79%) underwent RECO. Follow-up examination focused on clinical examination, complications and revision rates. Anteroposterior instability and ROM deficits were analyzed in order to evaluate our policy of early intervention for all cases of ROM restrictions. Results: Relevant ROM restrictions occurred at a significantly higher rate after DIS than after RECO (4.8% vs. 1.3%; p = 0.008). Flexion was more restricted after DIS than RECO (110° vs. 124°, p < 0.001). Extension deficits also occurred more frequently after DIS compared to RECO (49.7% vs. 24.5%; p < 0.001). Total revision surgery rate was 9.1%, with patients after DIS being significantly more frequently affected (20.4% vs. 6.1%; p < 0.001). Conclusions: Our findings indicate a significantly higher risk for ROM restriction after DIS compared to RECO, resulting in a significantly higher revision rate.
NDVI-Based Vegetation Dynamics and Their Responses to Climate Change and Human Activities from 2000 to 2020 in Miaoling Karst Mountain Area, SW China
Authors: Wu Yangyang , Yang Jinli , Li Siliang , Guo Chunzi , Yang Xiaodong , Xu Yue , Yue Fujun , Peng Haijun , Chen Yinchuan , Gu Lei , Shi Zhenghua , Luo Guangjie
Published: June 2023

Understanding spatiotemporal shifts in vegetation and their climatic and anthropogenic regulatory factors can offer a crucial theoretical basis for environmental conservation and restoration. In this article, the normalized difference vegetation index (NDVI) of the Miaoling area from 2000 to 2020 is studied using a trend analysis and the Mann–Kendall mutation test (MK test) to review the vegetation’s dynamic changes. Our study uses the Hurst index, a partial correlation analysis, and a geographic detector to investigate the contributions of climate change and human activities to regional vegetation changes and their drivers. We found that Miaoling’s annual average NDVI was between 0.66 and 0.83 in 2000–2020, with a mean of 0.766. The overall trend was slow upward (0.0009/year), and 53.82% of the region continued to grow and gradually increased from west to east in the spatial domain, among which the karst regional NDVI distribution area and its growth rate were higher than those of non-karst sites. Based on correlations between climatic factors and NDVI, precipitation seasonality (coefficient of variation, CV) had the strongest correlation (positive correlation) with NDVI, while vapor pressure deficit (VPD) had a negative correlation with NDVI. In the interaction, human activities played a dominant role in the influence of NDVI on the vegetation of Miaoling. The night light index had the most explanatory power on the NDVI (q = 0.422), and the interaction between anthropogenic factors and other factors dominated its explanatory power. This study has academic and practical importance for the management, protection, and sustainable development of karst basins.
Creep Behavior of CLT Beams with Finite Thickness Layers of Flexible Adhesives
Authors: Śliwa-Wieczorek Klaudia , Szeptyński Paweł , Kozik Tomasz , Gubert Martino
Published: June 2023

Creep behavior of Cross-Laminated-Timber (CLT) beams with a finite-thickness layer of flexible adhesives is investigated. Creep tests were carried out for all component materials as well as for the composite structure itself. Three-point bending creep tests were performed for spruce planks and for CLT beams, and uniaxial compression creep tests were performed for two flexible polyurethane adhesives: Sika® PS and Sika® PMM. All materials are characterized with the use of the three-element Generalized Maxwell Model. The results of creep tests for component materials were used in elaboration of the Finite Element (FE) model. The problem of linear theory of viscoelasticity was solved numerically with the use of the Abaqus software. Obtained results of Finite Element Analysis (FEA) are compared with experimental results.
A Novel Inorganic Aluminum Phosphate-Based Flame Retardant and Thermal Insulation Coating and Performance Analysis
Authors: Cai Guoshuai , Wu Jiaxuan , Guo Jiayi , Wan Yange , Zhou Qingjun , Zhang Pengyu , Yu Xiaolei , Wang Mingchao
Published: June 2023

Currently, most thin-layer expandable coatings are polymer-based, with very few inorganic expandable coatings. Due to the high environmental friendliness of inorganic coatings, studying new types of inorganic coatings is of great significance. A novel amorphous aluminum phosphate-based flame-retardant coating was prepared by modifying it with nano-silica, hollow silica beads, hollow glass microspheres, and boron carbide. A comprehensive study was conducted on the flame retardancy and thermal insulation performance, composition and structural evolution under flame and physical and chemical properties, and the mechanisms of flame retardancy and thermal insulation were elucidated. Large-plate combustion testing, bonding strength testing, XRD, IR, TG-DSC, and SEM testing were all applied in this work. The synergistic effect of the four fillers was very obvious, and a series of AP22XY (nano-silica/silica beads/hollow glass microspheres/boron carbide = 2:2:0:4, 2:2:1:3, 2:2:2:2, 2:2:3:1, 2:2:4:0) coatings were prepared. The change in the ratio of glass microspheres to boron carbide had a significant impact on the composition and structural evolution of the coating, thus reflecting its effectiveness as a flame retardant and thermal insulation. Although decreasing the ratio would promote the formation of borosilicate glass and Al18B4O33 and improve the thermal stability of coatings, the structure inside of the coating, especially the skeleton, would be dense, which is not conducive to thermal insulation. When the ratio of glass microspheres to boron carbide is 3:1, AP2231 shows the best fire resistance. Under the combustion of butane flame at about 1200–1300 °C, the backside temperature reaches a maximum of 226 °C at 10 min, and then the temperature gradually decreases to 175 °C at 60 min. This excellent performance is mainly attributed to three aspects: (1) the foaming and expandability of coatings when exposed to fire, (2) the multiple endothermic reactions the coating undergoes, and (3) the improvement effect of boron carbide. Additionally, AP2231 shows the best bonding performance with a strength of close to 4.5 MPa after combustion, because of the appropriate content matching between borosilicate glass, Al18B4O33, and hollow glass microspheres. The coating has potential application prospects in the construction and transportation fields, such as the protection of structural steel, fire prevention in subways and tunnels, and the prevention of lithium battery fires.
Nonlinear Electromagnetic Properties of Thinfilm Nanocomposites (CoFeZr)x(MgF2)100−x
Authors: Domashevskaya Evelina P. , Ivkov Sergey A. , Seredin Pavel V. , Goloshchapov Dmitry L. , Barkov Konstantin A. , Ryabtsev Stanislav V. , Segal Yrii G. , Sitnikov Alexander V. , Ganshina Elena A.
Published: June 2023

The aim of this work is a comprehensive study of the effect of variable atomic composition and structural-phase state of (CoFeZr)x(MgF2)100−x nanocomposites (NCs) on their nonlinear electronic and magnetic/magneto-optical properties. Micrometer-thick nanocomposite layers on the glass substrates were obtained by ion-beam sputtering of a composite target in the argon atmosphere in a wide range of compositions x = 9–51 at·%. The value of the resistive percolation threshold, xper = 34 at·%, determined from the concentration dependencies of the electrical resistance of NCs, coincides with the beginning of nucleation of metallic nanocrystals CoFeZr in MgF2 dielectric matrix. The absolute value of maximum magnetoresistance of NCs is 2.4% in a magnetic field of 5.5 kG at x = 25 at·%, up to the percolation threshold. Two maxima appear in the concentration dependencies of magneto-optical transversal Kerr effect, one of which, at x = 34 at·%, corresponds to the formation of CoFeZr alloy nanocrystals of a hexagonal structure, and the second one at x = 45 at·% corresponds to the phase transition of nanocrystals from a hexagonal to a cubic body-centered structure. The magnetic percolation threshold in (CoFeZr)x(MgF2)100−x system at xfm = 34 at·%, with the appearance of a hysteresis loop and a coercive force of Hc ≈ 8 Oe, coincides with the resistive percolation threshold xper = 34 at·%. Concentration dependence of the coercive force showed that at low contents of metallic alloy x < 30 at·%, NCs are superparamagnetic (Hc = 0). With an increase of the alloy content, in the region of magnetic and resistive percolation thresholds, NCs exhibit a magnetically soft ferromagnetic character and do not change it far beyond the percolation threshold, with the maximum value of the coercive force Hc < 30 Oe.
Expanding the Utility of Bioinformatic Data for the Full Stereostructural Assignments of Marinolides A and B, 24- and 26-Membered Macrolactones Produced by a Chemically Exceptional Marine-Derived Bacterium
Authors: Kim Min Cheol , Winter Jaclyn M. , Cullum Reiko , Smith Alexander J. , Fenical William
Published: June 2023

Marinolides A and B, two new 24- and 26-membered bacterial macrolactones, were isolated from the marine-derived actinobacterium AJS-327 and their stereostructures initially assigned by bioinformatic data analysis. Macrolactones typically possess complex stereochemistry, the assignments of which have been one of the most difficult undertakings in natural products chemistry, and in most cases, the use of X-ray diffraction methods and total synthesis have been the major methods of assigning their absolute configurations. More recently, however, it has become apparent that the integration of bioinformatic data is growing in utility to assign absolute configurations. Genome mining and bioinformatic analysis identified the 97 kb mld biosynthetic cluster harboring seven type I polyketide synthases. A detailed bioinformatic investigation of the ketoreductase and enoylreductase domains within the multimodular polyketide synthases, coupled with NMR and X-ray diffraction data, allowed for the absolute configurations of marinolides A and B to be determined. While using bioinformatics to assign the relative and absolute configurations of natural products has high potential, this method must be coupled with full NMR-based analysis to both confirm bioinformatic assignments as well as any additional modifications that occur during biosynthesis.
Optical Properties and Interference Effects of the Lens Mitochondrion
Authors: Margadant Felix , Almsherqi Zakaria , Xu Xiaochun , Deng Yuru
Published: June 2023

The lens mitochondrion of the tree shrew, located along the optical pathway between the lens and photoreceptors, has been investigated. The results suggest that the lens mitochondrion acts as a quasi-bandgap or imperfect photonic crystal. Interference effects cause a shift in the focus and introduce wavelength-dependent behavior similar to dispersion. Optical channels within the mitochondrion form a mild waveguide, preferentially propagating light within certain compartments. The lens mitochondrion also functions as an imperfect UV-shielding interference filter. Overall, this study provides insights into the dual role of the lens mitochondrion and the complex behavior of light within biological systems.
Preparation of PVDF-PVP Composite Membranes for Oily Wastewater Treatment
Authors: Kartohardjono Sutrasno , Salsabila Ghofira Muna Khansa , Ramadhani Azzahra , Purnawan Irfan , Lau Woei Jye
Published: June 2023

The oil and gas industry and related applications generate large quantities of oily wastewater, which can adversely affect the environment and human health if not properly handled. This study aims to prepare polyvinylidene fluoride (PVDF) membranes incorporated with polyvinylpyrrolidone (PVP) additives and utilize them to treat oily wastewater through the ultrafiltration (UF) process. Flat sheet membranes were prepared using PVDF dissolved in N,N-dimethylacetamide, followed by the addition of PVP ranging from 0.5 to 35 g. Characterization by scanning electron microscopy (SEM), water contact angle, Fourier transform infrared spectroscopy (FTIR), and mechanical strength tests were performed on the flat PVDF/PVP membranes to understand and compare the changes in the physical and chemical properties of the membranes. Prior to the UF process, oily wastewater was treated by a coagulation–flocculation process through a jar tester using polyaluminum chloride (PAC) as a coagulant. Based on the characterization of the membrane, the addition of PVP improves the physical and chemical properties of the membrane. The membrane’s pore size becomes larger, which can increase its permeability and flux. In general, the addition of PVP to the PVDF membrane can increase the porosity and decrease the water contact angle, thereby increasing the membrane’s hydrophilicity. With respect to filtration performance, the wastewater flux of the resultant membrane increases with increasing PVP content, but the rejections for TSS, turbidity, TDS, and COD are reduced.
A New Approach to the Development of Hollow Fiber Membrane Modules for Water Treatment: Mixed Polymer Matrices
Authors: da Silva Biron Dionísio , Espíndola Jonathan Cawettiere , Subtil Eduardo Lucas , Mierzwa José Carlos
Published: June 2023

In this study, mixed matrix hollow fiber polymeric membranes were prepared using polyethersulfone (PES) and polyvinylidene fluoride (PVDF) as polymers in their composition. N-methyl-2-pyrrolidone (NMP) was used as a solvent and demineralized water with an electrical conductivity below 3 μS·cm−1 was used as a non-solvent. A new approach to producing enhanced polymeric hollow fiber membranes based on the preparation of a simple blend PVDF/PES solution, and on the conformation of the composite membranes through the extrusion technique followed by the phase inversion process in a non-solvent bath, was applied. The investigation focused on the preparation of polymeric membranes with different polymer ratios and further assessment of the effects of these proportions on the membrane performance and in specific physical properties. The amount of PVDF ranged from 10 to 90% with 10% steps. The presence of PVDF, although it increased the membranes’ plasticity, had a negative effect on the overall mechanical properties of the composite membranes. Scanning electron microscopy (SEM) results showed good dispersion of both polymers in the polymeric matrix. Furthermore, the membrane permeability showed a slight negative correlation with contact angle, suggesting that membrane hydrophilicity played an important role in membrane permeability. Finally, it was found that membranes with low ratios of PVDF/PES may have potential for water treatment applications, due to the combined advantageous properties of PES and PVDF.
GOMEA: A Conceptual Design of a Membrane Electrode Assembly for a Proton Exchange Membrane Electrolyzer
Authors: Berning Torsten , Bessarabov Dmitri
Published: June 2023

We are proposing a conceptual membrane electrode assembly (MEA) of a proton exchange membrane water electrolyzer that includes a layer of graphene oxide (GO) at the cathode side. This GO layer primarily reinforces the MEA to allow operation at a higher pressure difference between the cathode and anode side. Additional benefits would be that a perfect GO layer would prevent both water and hydrogen crossover and thus would allow for pure, dry hydrogen escaping directly from the electrolyzer without losses due to hydrogen crossover, thus eliminating the need for hydrogen clean-up steps. The mechanical strength of graphene will also allow for a thinner polymer electrolyte membrane and could thus save cost. Finally, the effect of electro–osmotic drag on the water content in such an MEA is discussed, and it is argued that it could lead to an oversaturated membrane, which is highly desirable.
The Influence of Foaming Agents on Aluminium Foam Cell Morphology
Authors: Rodinger Tomislav , Ćorić Danko , Alar Željko
Published: June 2023

The choice of foaming agent and its mass fraction significantly affect the size and number of metal foam cells. The powder metallurgy process was used to produce aluminium foams with the addition of various foaming agents: titanium hydride (TiH2) and calcium carbonate (CaCO3). TiH2 was added in an amount of 0.4 wt.%, while the quantity of CaCO3 varied between 3 and 5 wt.%. The produced foams, with approximately the same degree of porosity, were scanned using a non-destructive computed tomography method. The number, size, equivalent diameter, sphericity, and compactness of cells were analysed on the obtained three-dimensional models. The results showed that foams foamed with TiH2 have much larger cells compared to CaCO3 agent. By considering the influence of CaCO3 fraction on the morphology of aluminium foam, it follows that a smaller quantity of CaCO3 (3 wt.%) provides a macrostructure with smaller cells. Samples with five wt.% CaCO3 contain slightly larger cells but are still much smaller than foams with TiH2 foaming agent at the same degree of porosity. The sphericity and compactness indicate that TiH2 foaming agent forms cells of a more regular shape compared to CaCO3 agent.
Rapid Fatigue Limit Estimation of Metallic Materials Using Thermography-Based Approach
Authors: Liu Zhanqi , Wang Haijiang , Chen Xueting , Wei Wei
Published: June 2023

This work attempts to develop a theoretical model in combination with the representative volume element (RVE) theory for realizing rapid fatigue limit prediction. Within the thermodynamic framework, it is believed that two components, namely anelastic and microplastic behaviors, which correspond to recoverable and non-recoverable microstructural motions, contribute to temperature variation during high-cycle fatigue. Based on this, the constitutive equation of the response relationship between the temperature rise evolution and the stress amplitude of metallic materials can be deduced in combination with the heat balance equation. Meanwhile, a determination approach for the thermographic experimental data for accurate fatigue limit estimation is developed by combining it with a statistical method. Finally, the experimental data of metallic specimens and welded joints were utilized to validate the proposed model, and the results demonstrated great agreement between experimental and predicted data.