bluuebunny/crossref_metadata_2025 huggingface dataset:
Solar nanophotocatalytic pretreatment of seawater: process optimization and performance evaluation using response surface methodology and genetic algorithm
Authors: Joy Varghese Manappallil , Feroz Shaik , Dutta Susmita
Published: January 2021

In reverse osmosis seawater treatment process, membrane fouling can be mitigated by degrading organic pollutants present in the feed seawater. The present study evaluates the effectiveness of employing solar photocatalysis using TiO2/ZnO/H2O2 to pretreat reverse osmosis (RO) feed seawater under solar irradiation. Process optimisation and performance evaluation were undertaken using response surface methodology-desirability function and RSM integrated with genetic algorithm (RSM-GA). Statistical analysis was performed to determine the interactive relationships and main effects of input factors such as TiO2 dosage, H2O2 dosage, pH, reaction time and ZnO dosage. The performance evaluation was determined in terms of percentage removal of total organic carbon (TOC) and chemical oxygen demand (COD). The obtained optimum values using RSM-GA evaluation for TOC and COD removal were found to be 76.5% and 63.9%, respectively. The predicted RSM-GA results correspond well with the experimental results (TOC removal = 73.3%, COD removal = 61.2%). Utilization of renewable solar energy coupled with optimum utilisation of nanophotocatalysts enables this technique to be a unique treatment process for RO pretreatment of seawater and membrane fouling mitigation.
Implementation of Quality by Design (QbD) Principles in Regulatory Dossiers of Medicinal Products in the European Union (EU) Between 2014 and 2019
Authors: ter Horst Judith P. , Turimella Sada L. , Metsers Frans , Zwiers Alex
Published: January 2021

Background

Quality by Design (QbD) is a systematic risk-based approach to development, with predefined characteristics and quality risk management throughout the life cycle of a product. International Conference on Harmonization (ICH) guidelines Q8–Q11 give guidance on QbD applications with ICH Q8 (R2)—approved in 2009—describing the principles of QbD in detail. Since its adoption over 10 years ago, more information about QbD usage for the development of medicinal products is expected to be written in regulatory dossiers by companies.

Methods

The present study set out to evaluate the implementation of QbD principles and elements in all EU approved marketing applications (MA) (n = 494), based on information available in the European Public Assessment Reports (EPARs), for a period of six years (2014–2019), starting 5 years after QbD adoption.

Results

Of the 494 MAs, 271 were submitted with a full dossier (article 8(3)). According to EMA (38%), out of the 271 full dossier submissions, only 104 were developed using full QbD. This figure did not increase during this period. Interestingly, between 2014 and 2019, several MAs were not developed via full QbD implementation but used one or more QbD elements during development, including design space. In addition, a higher percentage of small molecule products were developed with QbD as opposed to biotechnology-derived products (78% vs. 22%, respectively).

Conclusion

Overall, QbD during development of medicinal products is still not commonly described in dossiers. However, more companies started mentioning QbD elements, thus making it a promising step toward QbD as the standard for development in the future.
Borehole inventory, groundwater potential and water quality studies in Ayede Ekiti, Southwestern Nigeria
Authors: Ige Olusegun O. , Ameh Hycent O. , Olaleye Ifeoluwa M.
Published: January 2021

This study aims at determining the state of government provided boreholes, evaluating groundwater potential and quality assessment within the Ayede Ekiti community. 12 Vertical Electrical Soundings (VES) were conducted using Schlumberger array in order to determine geoelectric layers and fracture attributes. Also, 12 water samples were collected from the study area to evaluate physicochemical characteristics of the groundwater. The study revealed average values of total depth of boreholes, static water levels and water column in the boreholes to be 18.77 m, 6.77 m and 11.99 m respectively. 70% of the boreholes are either abandoned, damaged or with evidence of corrosion and encrustation. Geophysical investigation revealed weathered layer thickness ranging from 1.3 to 34.7 m with two regimes of fracture at 40–50 and 75–80 m. The frequency of curve types obtained shows 16.67%, 33.33%, 25%, 8.33%, 8.33% and 8.33% for AK, HA, KH, AA, QH and HK respectively while weathered and fractured basement are identified as the two types of aquifer unit. Results of water analysis unveiled that dominance cations are in order Ca2+ > Na+ > K+ > Mg2+while anions are in the order of HCO3− > Cl− > SO42−. Three types of hydrochemical facies present are CaHCO3 > NaHCO3 > CaCl in 66.67%, 25% and 8.33% respectively. The Wilcox plot suggests the suitability of the groundwater samples for irrigation purposes when compared with the World Health Organization standards. Despite potential for groundwater and good quality of analyzed samples, the problem of water in this community is traceable to inadequacy in exploration, shallow boreholes with consequent seasonal water availability.
Cavernous Branched Nerve Regeneration Using Non-Tubular Artificial Nerve Sheets Using Freeze-Dried Alginate Gel Combined With Polyglycolic Acid Mesh in a Rat Model
Authors: Narita Shintaro , Obara Takashi , Ishikawa Namiko , Suzuki Yoshihisa , Habuchi Tomonori
Published: January 2021

Introduction

Neuroprotection and neuroregeneration of cavernous nerve plexus by biological/bioengineering solutions may have the potential to maintain erectile function.

Aims

We evaluated the efficacy of a newly developed artificial nerve sheet using freeze-dried alginate (ALG) with polyglycolic acid (PGA) mesh in a rat model. Methods: Bilateral cavernous nerves of male rats were excised to make an approximately 2 mm gap. A piece of the sponge-like freeze-dried sheet created by covalent cross-linking of ALG gel combined with PGA mesh was placed over the gap to cover each stump without any neural anastomosis. We compared erectile functions in the ALG groups with those in the sham group and the bilateral nerve excision group (n = 12, each).

Main Outcome Measures

Main outcome measure was a rat model with cavernous nerve excision.

Results

All rats in the sham group had erection at 63 or 64 days, and mating behavior was confirmed in 10 rats (83.3%) of the sham group at 56 to 62 days. No erection and mating behavior was observed in the excision group. Ten of the 12 (83.3%) rats in the ALG group had a mating behavior and an erection, and the rates of erection and mating behavior were significantly higher in the ALG group than those in the excision group (P < .01, P < .01, respectively). Using a retrograde FluoroGold, the rate of FluoroGold positive pelvic ganglia proximal to the gap at 61 or 62 days was significantly higher in the ALG group than that in the excision group (P = .014).

Conclusion

The results of our animal study have demonstrated that simply filling the cavernous nerve gap using the non-tubular artificial nerve sheets made of ALG with PGA mesh restored erectile function after cavernous nerve excision.
Bayesian Econometric Methods
Authors: Chan Joshua , Koop Gary , Poirier Dale J. , Tobias Justin L.
Published: January 2021

Bayesian Econometric Methods examines principles of Bayesian inference by posing a series of theoretical and applied questions and providing detailed solutions to those questions. This second edition adds extensive coverage of models popular in finance and macroeconomics, including state space and unobserved components models, stochastic volatility models, ARCH, GARCH, and vector autoregressive models. The authors have also added many new exercises related to Gibbs sampling and Markov Chain Monte Carlo (MCMC) methods. The text includes regression-based and hierarchical specifications, models based upon latent variable representations, and mixture and time series specifications. MCMC methods are discussed and illustrated in detail - from introductory applications to those at the current research frontier - and MATLAB® computer programs are provided on the website accompanying the text. Suitable for graduate study in economics, the text should also be of interest to students studying statistics, finance, marketing, and agricultural economics.
Committed to Rights
Authors: Comstock Audrey L.
Published: January 2021

International treaties are the primary means for codifying global human rights standards. However, nation-states are able to make their own choices in how to legally commit to human rights treaties. A state commits to a treaty through four commitment acts: signature, ratification, accession, and succession. These acts signify diverging legal paths with distinct contexts and mechanisms for rights change reflecting legalization, negotiation, sovereignty, and domestic constraints. How a state moves through these actions determines how, when, and to what extent it will comply with the human rights treaties it commits to. Using legal, archival, and quantitative analysis this important book shows that disentangling legal paths to commitment reveals distinct and significant compliance outcomes. Legal context matters for human rights and has important implications for the conceptualization of treaty commitment, the consideration of non-binding commitment, and an optimistic outlook for the impact of human rights treaties.
Molecular Astrophysics
Authors: Tielens A. G. G. M.
Published: January 2021

Focusing on the organic inventory of regions of star and planet formation in the interstellar medium of galaxies, this comprehensive overview of the molecular universe is an invaluable reference source for advanced undergraduates through to entry-level researchers. It includes an extensive discussion of microscopic physical and chemical processes in the universe; these play a role in the excitation, spectral characteristics, formation, and evolution of molecules in the gas phase and on grain surfaces. In addition, the latest developments in this area of molecular astrophysics provide a firm foundation for an in-depth understanding of the molecular phases of the interstellar medium. The physical and chemical properties of gaseous molecules, mixed molecular ices, and large polycyclic aromatic hydrocarbon molecules and fullerenes and their role in the interstellar medium are highlighted. For those with an interest in the molecular universe, this advanced textbook bridges the gap between molecular physics, astronomy, and physical chemistry.
The Scholastic Project
Authors: Monagle Clare
Published: January 2021

This is a somewhat polemical, and very passionate, plea for more work not only about the house that scholasticism built, but those who were excluded from it. This book is the story of how scholastic theology defined this universal subject in terms of the reasonable white man and a catalogue of the exclusions which ensued. The categories of woman, Jew and heretic were core others against which ideal Christian subjectivity was implicitly defined, and this book shows just how constitutive these 'others' were for the production of orthodoxy in the Middle Ages.
The Peace of God
Authors: Koziol Geoffrey
Published: January 2021

The Peace of God was one of the most important movements of the Middle Ages, yet is highly contested. It has been seen as both radically innovative and fundamentally traditional; as millenarian and not millenarian; as a popular movement and as a way of consolidating power for the elites. Geoffrey Koziol argues for the validity of all these differing viewpoints, because specific instantiations of the Peace of God varied greatly, and there were inherent contradictions in early ideas of peace and peace-making.
From War to the Rule of Law
Authors: Voorhoeve Joris
Published: January 2021

As recent events in Iraq demonstrate, countries that have suffered through civil war or rule by military regime can face a long, difficult transition to peaceful democracy.

Drawing on the experiences of peacekeepers in Bosnia, Haiti, Rwanda, and Afghanistan, From War to Rule of Law demonstrates that newly emerging democracies may need much more than emergency economic support. Restoring the rule of law, Joris Voorhoeve shows, can involve the training of a new police force, for example, or the creation of an international war crimes tribunal. Any disregard for human rights or delay in civilian reconciliation can lead to serious resurgences in violence.

Voorhoeve concludes by offering specific recommendations for members of the United Nations and the European Union, as well as individual donors. Given the nature of today's armed conflicts, From War to Rule of Law provides new hope for all those concerned about the lasting success of international peacekeeping missions.
The Crisis Imperative
Authors: Kuipers Sanneke
Published: January 2021

Belgium and the Netherlands were perfect examples of the 'welfare without work' policy that characterized European welfare states-until a political crisis in both countries during the early 1990s produced a surprising divergence in administration. While Belgium's government announced major reforms, its social security policy remained relatively resilient. In the Netherlands, however, policymakers implemented unprecedented cutbacks as well as a major overhaul of the disability benefits program. The Crisis Imperative explains this difference as the result of crisis rhetoric-that is, the deliberate construction of a crisis as the imperative for change. It will be a valuable resource for policymakers, researchers, and anyone interested in welfare reform in the United States and abroad.
Media Policy for the Digital Age
Authors: Netherlands Scientific Council for Government Policy The
Published: January 2021

Traditionally, the Netherlands has enjoyed status as a test market for new media. But in the past decade, such innovations have been severely hampered by questions about the future of public broadcasting. This issue has led to abundant political grandstanding, but little in the way of definitive policymaking. In February 2005, the Scientific Council for Government Policy published a report with practical policy suggestions. Media Policy for the Digital Age summarizes the Council's recommendations, giving readers outside the Netherlands insight into the issues at stake and possible solutions, as well as a concise analysis that tackles the challenges of making robust media policy for the twenty-first century.
From Indifference to Entrapment
Authors: Both Norbert
Published: January 2021

A detailed analysis of the response to the Yugoslav crisis by one of America's key allies in NATO. The author focuses on the question of how a Western bureaucracy faced up to the most complex foreign policy challenge of the 1990s. The Netherlands, as a 'pocket-sized medium power', is an interesting case study. While the margins for Dutch foreign policy are limited, fate had it that the Netherlands occupied the European presidency during the second half of 1991, when the recognition issue divided the West and the parameters for the subsequent international intervention in the Balkans were set. By July 1995, the involvement of the Netherlands had deepened to the extent that Dutch troops who found themselves trapped in the UN safe area of Srebrenica together with the local Muslim population were unable to prevent the worst massacre in Europe since the Second World War.

This study is based on interviews with all the major players, including two former Defence Ministers and two former Ministers of Foreign Affairs, and on documents from the Netherlands Ministry of Foreign Affairs, made available under the country's own 'freedom of information act'.
Art in Progress
Authors: Doorman Maarten
Published: January 2021

In this challenging essay, Maarten Doorman argues that in art, belief in progress is still relevant, if not essential. The radical freedoms of postmodernism, he claims, have had a crippling effect on art, leaving it in danger of becoming meaningless. Art can only acquire meaning through context; the concept of progress, then, is ideal as the primary criterion for establishing that context. The history of art, in fact, can be seen as a process of constant accumulation, works of art commenting on one another and enriching one another's meanings. It is these complex interrelationships and the progress they create in both art and its observers that Doorman, in a display of great philosophical erudition, defends.
Herr Lubitsch Goes to Hollywood
Authors: Thompson Kristin
Published: January 2021

The first study by an acclaimed American scholar of the artistic interdependencies between the German and the Hollywood cinema in the 1920s
A class of continuous non-associative algebras arising from algebraic groups including
Authors: Chayet Maurice , Garibaldi Skip
Published: January 2021

We give a construction that takes a simple linear algebraic group G over a field and produces a commutative, unital, and simple non-associative algebra A over that field. Two attractions of this construction are that (1) when G has type

$E_8$

, the algebra A is obtained by adjoining a unit to the 3875-dimensional representation; and (2) it is effective, in that the product operation on A can be implemented on a computer. A description of the algebra in the

$E_8$

case has been requested for some time, and interest has been increased by the recent proof that

$E_8$

is the full automorphism group of that algebra. The algebras obtained by our construction have an unusual Peirce spectrum.
COVID-19 pandemic: influence of relationship status on stress, anxiety, and depression in Canada
Authors: Nkire Nnamdi , Nwachukwu Izu , Shalaby Reham , Hrabok Marianne , Vuong Wesley , Gusnowski April , Surood Shireen , Greenshaw Andrew J. , Agyapong Vincent I. O.
Published: January 2021

Objective:

To examine the impact of relationship status on levels of stress, anxiety, and depression during the coronavirus (COVID-19) pandemic to identify relationship status groups who are at greater risk of mental health difficulties.

Methods:

The sample was drawn from individuals who subscribed to the Text4Hope program, a cognitive behavioral therapy inspired text messaging service developed to support Albertans during the COVID-19 pandemic. A survey link was sent to the subscribers to ascertain their relationship status and assess psychopathology using the Perceived Stress Scale-10 (PSS-10), Generalized Anxiety Disorder 7-item (GAD-7) scale, and Patient Health Questionnaire-9 (PHQ-9). Data analysis was carried out using SPSS-26 for descriptive statistics.

Results:

Within the first 6 weeks of the pandemic, 8267 of 44·992 subscribers responded to the online survey giving a response rate of 19.4%. Mean scores on the PSS, GAD-7, and PHQ-9 were highest among those who were single and lowest among those who were widowed. Overall, mean scores on the PHQ-9 were higher in groups who self-identified as separated or divorced when compared with groups who identified as having partners, including the categories of married or cohabiting.

Conclusions:

Relationship status during the COVID-19 pandemic has an influence on the mental health of individuals. Our findings highlight relationship groups at risk of mental health problems during the pandemic and for whom treatments and mitigation should be targeted.
Changes in diet quality and body weight over 10 years: the Multiethnic Cohort Study
Authors: Kang Minji , Boushey Carol J. , Shvetsov Yurii B. , Setiawan Veronica W. , Paik Hee-Young , Wilkens Lynne R. , Le Marchand Loic , Park Song-Yi
Published: January 2021

High-quality diets have been found to be beneficial in preventing long-term weight gain. However, concurrent changes in diet quality and body weight over time have rarely been reported. We examined the association between 10-year changes in diet quality and body weight in the Multiethnic Cohort Study. Analyses included 53 977 African Americans, Native Hawaiians, Japanese Americans, Latinos and Whites, who completed both baseline (1993–1996, 45–69 years) and 10-year follow-up (2003–2008) surveys including a FFQ and had no history of heart disease or cancer. Using multivariable regression, weight changes were regressed on changes in four diet quality indexes, Healthy Eating Index-2015, Alternative Healthy Eating Index-2010, alternate Mediterranean Diet and Dietary Approaches to Stop Hypertension scores. Mean weight change over 10 years was 1·2 (sd 6·8) kg in men and 1·5 (sd 7·2) kg in women. Compared with stable diet quality (< 0·5 sd change), the greatest increase (≥ 1 sd increase) in the diet scores was associated with less weight gain (by 0·55–1·17 kg in men and 0·62–1·31 kg in women). Smaller weight gain with improvement in diet quality was found in most subgroups by race/ethnicity, baseline age and baseline BMI. The inverse association was stronger in younger age and higher BMI groups. Ten-year improvement in diet quality was associated with a smaller weight gain, which varied by race/ethnicity and baseline age and BMI. Our findings suggest that maintaining a high-quality diet and improving diet quality over time may prevent excessive weight gain.
Modelling effects of edge density fluctuations on electron-cyclotron current drive used for neoclassical tearing mode stabilization
Authors: Tsironis C. , Papagiannis P. , Bairaktaris F. , Valvis S. I. , Hizanidis K.
Published: January 2021

In this work we study the undesired effects of electron density fluctuations (in the form of blob structures which may exist in the edge region of tokamak plasmas) to the electron-cyclotron wave propagation and current drive in connection to the efficiency of neoclassical tearing mode stabilization. Our model involves the evaluation of the driven current in the presence of density perturbations, by using a combination of a wave solver based on the transfer matrix and electromagnetic homogenization methods for the propagation part prior to and inside the region of these structures (where standard asymptotic propagation methods may not be valid due to the short-wavelength limit breakdown), with a ray tracing code including island geometry effects and current drive computation for the propagation past the perturbed region. The computed driven current is input into the modified Rutherford equation in order to estimate the consequences of the wave deformation (driven by the density fluctuations) to the mode stabilization.
The community structure of hyperiid amphipods associated with two seamount regions in the South-east Pacific
Authors: Espinosa-Leal Liliana , Medellín-Mora Johanna , Corredor-Acosta Andrea , Escribano Rubén
Published: January 2021

Oceanic islands and seamounts are considered biodiversity hotspots. Here, we present a taxonomy and community analyses of hyperiid amphipods collected near oceanic islands and over seamounts of the Juan Fernández Archipelago and Desventuradas Archipelago in the South-east Pacific. Both archipelagos are separated by about 800 km over the meridional gradient, suggesting the existence of different hyperiid communities because of apparent geographic isolation and distinctive oceanographic characteristics between regions. To test this hypothesis, zooplankton samples were collected from 19 stations during the CIMAR 22 ‘Oceanic Island’ cruise in October–November 2016. In total, 56 species of hyperiids were found, of whichPhrosina semilunata,Lestrigonus schizogeneios,Hyperietta stephenseni,Hyperioides longipes,Phronimella elongataandPrimno latreilleiwere the most abundant and recurrent species. The species richness (S), Shannon–Wiener diversity (H’) and dominance (D) of both the archipelagos were not significantly different. Additionally, except for a small group of rare species, the species composition was similar in both areas. Most species showed greater abundances than those observed in the coastal upwelling zone off Chile, whereas shared species between regions suggested the presence of a single biogeographic unit comprising the coastal transition zone and oceanic area off Chile within which both archipelagos are included. Correlation analysis indicated that salinity was the best predictor for the community structure, which provides evidence that the contributions of previously described water masses of the South-east Pacific may influence the spatial distribution and composition of the hyperiid community.
The impact of smoking status on cognition and brain morphology in schizophrenia spectrum disorders
Authors: Ringin Elysha , Cropley Vanessa , Zalesky Andrew , Bruggemann Jason , Sundram Suresh , Weickert Cynthia Shannon , Weickert Thomas W. , Bousman Chad A. , Pantelis Christos , Van Rheenen Tamsyn E.
Published: January 2021

Background

Cigarette smoking is associated with worse cognition and decreased cortical volume and thickness in healthy cohorts. Chronic cigarette smoking is prevalent in schizophrenia spectrum disorders (SSD), but the effects of smoking status on the brain and cognition in SSD are not clear. This study aimed to understand whether cognitive performance and brain morphology differed between smoking and non-smoking individuals with SSD compared to healthy controls.

Methods

Data were obtained from the Australian Schizophrenia Research Bank. Cognitive functioning was measured in 299 controls and 455 SSD patients. Cortical volume, thickness and surface area data were analysed from T1-weighted structural scans obtained in a subset of the sample (n= 82 controls,n= 201 SSD). Associations between smoking status (cigarette smoker/non-smoker), cognition and brain morphology were tested using analyses of covariance, including diagnosis as a moderator.

Results

No smoking by diagnosis interactions were evident, and no significant differences were revealed between smokers and non-smokers across any of the variables measured, with the exception of a significantly thinner left posterior cingulate in smokers compared to non-smokers. Several main effects of smoking in the cognitive, volume and thickness analyses were initially significant but did not survive false discovery rate (FDR) correction.

Conclusions

Despite the general absence of significant FDR-corrected findings, trend-level effects suggest the possibility that subtle smoking-related effects exist but were not uncovered due to low statistical power. An investigation of this topic is encouraged to confirm and expand on our findings.
ON EXTROPY PROPERTIES AND DISCRIMINATION INFORMATION OF DIFFERENT STRATIFIED SAMPLING SCHEMES
Authors: Eftekharian Abbas , Qiu Guoxin
Published: January 2021

Ranked set sampling (RSS) and some of its variants are sampling designs that are applied widely in different areas. When the underlying population contains different subpopulations, we can use stratified ranked set sampling (SRSS) which combines the advantages of stratification with RSS. In the present paper, we consider the information content of SRSS in terms of extropy measure. Some results using stochastic orders properties are obtained. The effect of imperfect ranking on discrimination information is analytically investigated. It is proved that discrimination information between the perfect SRSS and simple random sampling (SRS) data sets performs better than that of between the imperfect SRSS and SRS data sets.
Cognitive Fatigue and Processing Speed in Children Treated for Brain Tumours
Authors: Irestorm Elin , Ora Ingrid , Linge Helena , Tonning Olsson Ingrid
Published: January 2021

Objective:

The relationship between fatigue and cognition has not been fully elucidated in children and adolescent survivors of brain tumours. The aim of the present study was to investigate the potential relationship between fatigue and cognitive impairments in these survivors, as this group is at risk for both types of deficits.

Methods:

Survivors of paediatric brain tumours (n = 45) underwent a neuropsychological testing on average 4 years after diagnosis. Mean age at follow-up was 13.41 years. Cognition was assessed with neuropsychological tests, and fatigue with the Pediatric Quality of Life (PedsQL™) Multidimensional Fatigue Scale. Regression analysis, adjusted for cranial radiotherapy and age at diagnosis, was used to investigate the associations between cognitive variables and fatigue subscales. Cognitive variables associated with fatigue were subsequently exploratively assessed.

Results:

Significant associations were found for cognitive fatigue and measures of cognitive processing speed; Coding: p = .003, r = .583, 95% CI [9.61; 22.83] and Symbol Search: p = .001, r = .585, 95% CI [10.54; 24.87]. Slower processing speed was associated with poorer results for cognitive fatigue. Survivors with the largest decrease in processing speed from baseline to follow-up also experienced the most cognitive fatigue. Survivors expressed more cognitive fatigue compared to other types of fatigue.

Conclusions:

The association between cognitive fatigue and cognitive processing speed in children and adolescents treated for brain tumours is in concordance with the results previously reported in adults. Some survivors experience fatigue without impairment in processing speed, indicating the need for comprehensive assessments. Moreover, the study supports that fatigue is a multidimensional concept which should be measured accordingly.
Students choosing fat-free chocolate milk during school lunch consume more calories, total sugar, protein, minerals and vitamins at lunch
Authors: Peckham Janet G , Kropp Jaclyn D , Mroz Thomas A , Haley-Zitlin Vivian , Granberg Ellen
Published: January 2021

Objective:

To examine how milk consumption varies by milk choice and measure the association of milk choice on the nutritional and energetic content of National School Lunch Program (NSLP) lunches.

Design:

An observational plate waste study using the Digital Photography of Foods Method.

Setting:

Data were collected from two suburban South Carolina schools in one district during February and March 2013.

Participants:

Totally, 968 NSLP lunches selected by 619 kindergarten to fifth grade students.

Results:

Most students chose chocolate milk (75 %). A multinomial logit model indicated milk choice varied significantly by sociodemographic characteristics. An ordinary least square regression indicated that consumption rates of low-fat white milk were 8·5 % lower than fat-free chocolate milk (P = 0·039) and milk consumption rates varied statistically by sociodemographic characteristics. Ordinary least square regressions found that the consumption of energies and nutrients from NSLP lunches varied with sociodemographic characteristics and milk choice; students selecting chocolate milk consumed 58 more energies (P < 0·001) and 10 more grams of total sugar (P < 0·001) than students selecting low-fat white milk from their NSLP lunches. Students consumed statistically similar energies and nutrients from the non-milk components of their meals.

Conclusions:

Students selecting chocolate milk consumed more energies and nutrients from their NSLP lunches with the increases in consumption attributed to the milk component of the meal. The findings have implications for recent changes to NSLP guidelines that allow schools to offer both low-fat and fat-free flavoured milk, reversing the previous ban on low-fat flavoured milk under the Healthy, Hunger-Free Kids Act.
Information and legitimacy: results from an experimental survey on attitudes to the 2017 pension reform in Finland
Authors: Kangas Olli , Airio Ilpo , Koskenvuo Karoliina , Kuivalainen Susan , Tenhunen Sanna
Published: January 2021

The legitimacy of a pension system or any social security program depends on its credibility and perceived fairness. In order to gauge this legitimacy, we need to understand the relation between people's knowledge and attitudes. This experimental survey into the role of knowledge and perceptions divided respondents into two groups: the ‘treatment’ group received an information letter about a forthcoming pension reform before they were interviewed, while the control group was interviewed without receiving this ‘treatment’. Comparisons of the responses from the two groups allow us to assess how the level of knowledge and the provision of information affect people's opinions on policy reform. We also consider the patterns of covariation between background factors, people's concerns, and attitudes toward pension reform. The results show that the information letter had a significant impact on subjective but not on the objective level of knowledge. Receiving the information letter improved acceptance and perceptions of the fairness of the reform.
High-fructose diet during puberty alters the sperm parameters, testosterone concentration, and histopathology of testes and epididymis in adult Wistar rats
Authors: Medaglia Daniele Sapede Alvarenga , Vieira Henrique Rodrigues , Silveira Sandra da Silva , Siervo Gláucia Eloisa Munhoz de L. , Marcon Monique Suellen da Silva , Mathias Paulo Cezar de Freitas , Fernandes Glaura S. A.
Published: January 2021

The consumption of fructose has increased in children and adolescents and is partially responsible for the high incidence of metabolic diseases. The lifestyle during postnatal development can result in altered metabolic programming, thereby impairing the reproductive system and fertility during adulthood. Therefore, the aim of this study was to evaluate the effect of a high-fructose diet in the male reproductive system of pubertal and adult rats. Male Wistar rats (30 d old) were assigned to four different groups: Fr30, which received fructose (20%) in water for 30 d and were euthanized at postnatal day (PND) 60; Re-Fr30, which received fructose (20%) for 30 d and were euthanized at PND 120; and two control groups C30 and Re-C30, which received water ad libitum and were euthanized at PND 60 and 120, respectively. Fructose induced an increase in abnormal seminiferous tubules with epithelial vacuoles, degeneration, and immature cells in the lumen. Moreover, Fr30 rats showed altered spermatogenesis and daily sperm production (DSP), as well as increased serum testosterone concentrations. After discontinuing high-fructose consumption, DSP and sperm number decreased significantly. We observed tissue remodeling in the epididymis, with a reduction in stromal and epithelial compartments that might have influenced sperm motility. Therefore, we concluded that fructose intake in peripubertal rats led to changes in the reproductive system observed both during puberty and adulthood.
Potential roles of stem cell marker genes in axon regeneration
Authors: Lee Jinyoung , Cho Yongcheol
Published: January 2021

Axon regeneration is orchestrated by many genes that are differentially expressed in response to injury. Through a comparative analysis of gene expression profiling, injury-responsive genes that are potential targets for understanding the mechanisms underlying regeneration have been revealed. As the efficiency of axon regeneration in both the peripheral and central nervous systems can be manipulated, we suggest that identifying regeneration-associated genes is a promising approach for developing therapeutic applications in vivo. Here, we review the possible roles of stem cell marker- or stemness-related genes in axon regeneration to gain a better understanding of the regeneration mechanism and to identify targets that can enhance regenerative capacity.
PU.1 drives specification of pluripotent stem cell-derived endothelial cells to LSEC-like cells
Authors: De Smedt Jonathan , van Os Elise Anne , Talon Irene , Ghosh Sreya , Toprakhisar Burak , Furtado Madeiro Da Costa Rodrigo , Zaunz Samantha , Vazquez Marta Aguirre , Boon Ruben , Baatsen Pieter , Smout Ayla , Verhulst Stefaan , van Grunsven Leo A. , Verfaillie Catherine M.
Published: January 2021

To date, there is no representative in vitro model for liver sinusoidal endothelial cells (LSECs), as primary LSECs dedifferentiate very fast in culture and no combination of cytokines or growth factors can induce an LSEC fate in (pluripotent stem cell (PSC)-derived) endothelial cells (ECs). Furthermore, the transcriptional programmes driving an LSEC fate have not yet been described. Here, we first present a computational workflow (CenTFinder) that can identify transcription factors (TFs) that are crucial for modulating pathways involved in cell lineage specification. Using CenTFinder, we identified several novel LSEC-specific protein markers, such as FCN2 and FCN3, which were validated by analysis of previously published single-cell RNAseq data. We also identified PU.1 (encoded by the SPI1 gene) as a major regulator of LSEC-specific immune functions. We show that SPI1 overexpression (combined with the general EC TF ETV2) in human PSCs induces ECs with an LSEC-like phenotype. The ETV2-SPI1-ECs display increased expression of LSEC markers, such as CD32B and MRC1, as well as several of the proposed novel markers. More importantly, ETV2-SPI1-ECs acquire LSEC functions, including uptake of FSA-FITC, as well as labelled IgG. In conclusion, we present the CenTFinder computational tool to identify key regulatory TFs within specific pathways, in this work pathways of lineage specification, and we demonstrate its use by the identification and validation of PU.1 as a master regulator for LSEC fating.
Adaptive resistance to PI3Kα-selective inhibitor CYH33 is mediated by genomic and transcriptomic alterations in ESCC cells
Authors: Wang Yu-xiang , Zhang Xu , Ma Qing-yang , Hu Lan-dian , Zhang Xi , Wang Yi , Xu Lan , Yang Chun-hao , Tan Cun , Kong Xiang-yin , Ding Jian , Meng Ling-hua
Published: January 2021

Phosphoinositide-3 kinase alpha-specific inhibitors (PI3Kαi) displayed promising potential for the treatment of esophageal squamous cell carcinoma (ESCC) with frequent activation in PI3K signaling. However, acquired resistance is likely to develop and limit the efficacy of PI3Kαi like other targeted therapies. To identify genomic adaptation to PI3Kαi, we applied whole-genome sequencing and detected gene mutation and amplification in four lines of ESCC cells established with adapted resistance to a novel PI3Kαi CYH33. Particularly, HRASG12S mutation was found in KYSE180C cells. Overexpression of HRASG12S in ESCC parental cells rendered resistance to CYH33. By contrast, down-regulation of HRASG12S restored the sensitivity of KYSE180C1 cells to CYH33, and combination of CYH33 and MEK162 displayed synergistic effect against KYSE180C1 cells and xenografts. Furthermore, elevated mTORC1, mitogen-activated protein kinase (MAPK), and c-Myc signaling pathways were found in resistant cells by RNA sequencing and combination of CYH33 and RAD001, MEK162, or OTX015 overcame the resistance to CYH33, which was accompanied with enhanced inhibition on S6, extracellular signal-regulated kinase 1 (ERK), or c-Myc, respectively. Overall, we characterized the adaptations to PI3Kαi in ESCC cells and identified combinatorial regimens that may circumvent resistance.
Novel TNFAIP3 microdeletion in a girl with infantile-onset inflammatory bowel disease complicated by a severe perianal lesion
Authors: Taniguchi Kosuke , Inoue Mikihiro , Arai Katsuhiro , Uchida Keiichi , Migita Osuke , Akemoto Yui , Hirayama Junya , Takeuchi Ichiro , Shimizu Hirotaka , Hata Kenichiro
Published: January 2021

A20 haploinsufficiency (HA20), a disease caused by loss-of-function TNFAIP3 mutations, manifests various autoinflammatory and/or autoimmune symptoms. Some cases of HA20 were initially diagnosed as very early onset inflammatory bowel disease (VEO-IBD). We performed whole-exome sequencing (WES) for a Japanese girl with infantile-onset IBD and a severe perianal lesion and detected a novel de novo 119 kb microdeletion containing only TNFAIP3 (arr[GRCh37] 6q23.3(138125829_138244816) × 1).
Identification of fidelity-governing factors in human recombinases DMC1 and RAD51 from cryo-EM structures
Authors: Luo Shih-Chi , Yeh Hsin-Yi , Lan Wei-Hsuan , Wu Yi-Min , Yang Cheng-Han , Chang Hao-Yen , Su Guan-Chin , Lee Chia-Yi , Wu Wen-Jin , Li Hung-Wen , Ho Meng-Chiao , Chi Peter , Tsai Ming-Daw
Published: January 2021

Both high-fidelity and mismatch-tolerant recombination, catalyzed by RAD51 and DMC1 recombinases, respectively, are indispensable for genomic integrity. Here, we use cryo-EM, MD simulation and functional analysis to elucidate the structural basis for the mismatch tolerance of DMC1. Structural analysis of DMC1 presynaptic and postsynaptic complexes suggested that the lineage-specific Loop 1 Gln244 (Met243 in RAD51) may help stabilize DNA backbone, whereas Loop 2 Pro274 and Gly275 (Val273/Asp274 in RAD51) may provide an open “triplet gate” for mismatch tolerance. In support, DMC1-Q244M displayed marked increase in DNA dynamics, leading to unobservable DNA map. MD simulation showed highly dispersive mismatched DNA ensemble in RAD51 but well-converged DNA in DMC1 and RAD51-V273P/D274G. Replacing Loop 1 or Loop 2 residues in DMC1 with RAD51 counterparts enhanced DMC1 fidelity, while reciprocal mutations in RAD51 attenuated its fidelity. Our results show that three Loop 1/Loop 2 residues jointly enact contrasting fidelities of DNA recombinases.
Emergency deployment of direct air capture as a response to the climate crisis
Authors: Hanna Ryan , Abdulla Ahmed , Xu Yangyang , Victor David G.
Published: January 2021

Though highly motivated to slow the climate crisis, governments may struggle to impose costly polices on entrenched interest groups, resulting in a greater need for negative emissions. Here, we model wartime-like crash deployment of direct air capture (DAC) as a policy response to the climate crisis, calculating funding, net CO2removal, and climate impacts. An emergency DAC program, with investment of 1.2–1.9% of global GDP annually, removes 2.2–2.3 GtCO2yr–1in 2050, 13–20 GtCO2yr–1in 2075, and 570–840 GtCO2cumulatively over 2025–2100. Compared to a future in which policy efforts to control emissions follow current trends (SSP2-4.5), DAC substantially hastens the onset of net-zero CO2emissions (to 2085–2095) and peak warming (to 2090–2095); yet warming still reaches 2.4–2.5 °C in 2100. Such massive CO2removals hinge on near-term investment to boost the future capacity for upscaling. DAC is most cost-effective when using electricity sources already available today: hydropower and natural gas with renewables; fully renewable systems are more expensive because their low load factors do not allow efficient amortization of capital-intensive DAC plants.
Two distinct catalytic pathways for GH43 xylanolytic enzymes unveiled by X-ray and QM/MM simulations
Authors: Morais Mariana A. B. , Coines Joan , Domingues Mariane N. , Pirolla Renan A. S. , Tonoli Celisa C. C. , Santos Camila R. , Correa Jessica B. L. , Gozzo Fabio C. , Rovira Carme , Murakami Mario T.
Published: January 2021

Xylanolytic enzymes from glycoside hydrolase family 43 (GH43) are involved in the breakdown of hemicellulose, the second most abundant carbohydrate in plants. Here, we kinetically and mechanistically describe the non-reducing-end xylose-releasing exo-oligoxylanase activity and report the crystal structure of a native GH43 Michaelis complex with its substrate prior to hydrolysis. Two distinct calcium-stabilized conformations of the active site xylosyl unit are found, suggesting two alternative catalytic routes. These results are confirmed by QM/MM simulations that unveil the complete hydrolysis mechanism and identify two possible reaction pathways, involving different transition state conformations for the cleavage of xylooligosaccharides. Such catalytic conformational promiscuity in glycosidases is related to the open architecture of the active site and thus might be extended to other exo-acting enzymes. These findings expand the current general model of catalytic mechanism of glycosidases, a main reaction in nature, and impact on our understanding about their interaction with substrates and inhibitors.
A structural polymer for highly efficient all-day passive radiative cooling
Authors: Wang Tong , Wu Yi , Shi Lan , Hu Xinhua , Chen Min , Wu Limin
Published: January 2021

All-day passive radiative cooling has recently attracted tremendous interest by reflecting sunlight and radiating heat to the ultracold outer space. While some progress has been made, it still remains big challenge in fabricating highly efficient and low-cost radiative coolers for all-day and all-climates. Herein, we report a hierarchically structured polymethyl methacrylate (PMMA) film with a micropore array combined with random nanopores for highly efficient day- and nighttime passive radiative cooling. This hierarchically porous array PMMA film exhibits sufficiently high solar reflectance (0.95) and superior longwave infrared thermal emittance (0.98) and realizes subambient cooling of ~8.2 °C during the night and ~6.0 °C to ~8.9 °C during midday with an average cooling power of ~85 W/m2 under solar intensity of ~900 W/m2, and promisingly ~5.5 °C even under solar intensity of ~930 W/m2 and relative humidity of ~64% in hot and moist climate. The micropores and nanopores in the polymer film play crucial roles in enhancing the solar reflectance and thermal emittance.
Effects of plasma turbulence on the nonlinear evolution of magnetic island in tokamak
Authors: Choi Minjun J. , Bardōczi Lāszlo , Kwon Jae-Min , Hahm T. S. , Park Hyeon K. , Kim Jayhyun , Woo Minho , Park Byoung-Ho , Yun Gunsu S. , Yoon Eisung , McKee George
Published: January 2021

Magnetic islands (MIs), resulting from a magnetic field reconnection, are ubiquitous structures in magnetized plasmas. In tokamak plasmas, recent researches suggested that the interaction between an MI and ambient turbulence can be important for the nonlinear MI evolution, but a lack of detailed experimental observations and analyses has prevented further understanding. Here, we provide comprehensive observations such as turbulence spreading into an MI and turbulence enhancement at the reconnection site, elucidating intricate effects of plasma turbulence on the nonlinear MI evolution.
SARS-CoV-2 spike glycoprotein vaccine candidate NVX-CoV2373 immunogenicity in baboons and protection in mice
Authors: Tian Jing-Hui , Patel Nita , Haupt Robert , Zhou Haixia , Weston Stuart , Hammond Holly , Logue James , Portnoff Alyse D. , Norton James , Guebre-Xabier Mimi , Zhou Bin , Jacobson Kelsey , Maciejewski Sonia , Khatoon Rafia , Wisniewska Malgorzata , Moffitt Will , Kluepfel-Stahl Stefanie , Ekechukwu Betty , Papin James , Boddapati Sarathi , Jason Wong C. , Piedra Pedro A. , Frieman Matthew B. , Massare Michael J. , Fries Louis , Bengtsson Karin Lövgren , Stertman Linda , Ellingsworth Larry , Glenn Gregory , Smith Gale
Published: January 2021

The COVID-19 pandemic continues to spread throughout the world with an urgent need for a safe and protective vaccine to effectuate herd protection and control the spread of SARS-CoV-2. Here, we report the development of a SARS-CoV-2 subunit vaccine (NVX-CoV2373) from the full-length spike (S) protein that is stable in the prefusion conformation. NVX-CoV2373 S form 27.2-nm nanoparticles that are thermostable and bind with high affinity to the human angiotensin-converting enzyme 2 (hACE2) receptor. In mice, low-dose NVX-CoV2373 with saponin-based Matrix-M adjuvant elicit high titer anti-S IgG that blocks hACE2 receptor binding, neutralize virus, and protects against SARS-CoV-2 challenge with no evidence of vaccine-associated enhanced respiratory disease. NVX-CoV2373 also elicits multifunctional CD4+ and CD8+ T cells, CD4+ follicular helper T cells (Tfh), and antigen-specific germinal center (GC) B cells in the spleen. In baboons, low-dose levels of NVX-CoV2373 with Matrix-M was also highly immunogenic and elicited high titer anti-S antibodies and functional antibodies that block S-protein binding to hACE2 and neutralize virus infection and antigen-specific T cells. These results support the ongoing phase 1/2 clinical evaluation of the safety and immunogenicity of NVX-CoV2373 with Matrix-M (NCT04368988).