bluuebunny/crossref_metadata_2025 huggingface dataset:
Radiological assessment of hip disease in children with cerebral palsy: development of a core measurement set
Authors: Joseph Prince J. S. , Khattak Mohammed , Masudi Sundas T. , Minta Louise , Perry Daniel C.
Published: November 2023

Aims

Hip disease is common in children with cerebral palsy (CP) and can decrease quality of life and function. Surveillance programmes exist to improve outcomes by treating hip disease at an early stage using radiological surveillance. However, studies and surveillance programmes report different radiological outcomes, making it difficult to compare. We aimed to identify the most important radiological measurements and develop a core measurement set (CMS) for clinical practice, research, and surveillance programmes.

Methods

A systematic review identified a list of measurements previously used in studies reporting radiological hip outcomes in children with CP. These measurements informed a two-round Delphi study, conducted among orthopaedic surgeons and specialist physiotherapists. Participants rated each measurement on a nine-point Likert scale (‘not important’ to ‘critically important’). A consensus meeting was held to finalize the CMS.

Results

Overall, 14 distinct measurements were identified in the systematic review, with Reimer’s migration percentage being the most frequently reported. These measurements were presented over the two rounds of the Delphi process, along with two additional measurements that were suggested by participants. Ultimately, two measurements, Reimer’s migration percentage and femoral head-shaft angle, were included in the CMS.

Conclusion

This use of a minimum standardized set of measurements has the potential to encourage uniformity across hip surveillance programmes, and may streamline the development of tools, such as artificial intelligence systems to automate the analysis in surveillance programmes. This core set should be the minimum requirement in clinical studies, allowing clinicians to add to this as needed, which will facilitate comparisons to be drawn between studies and future meta-analyses.

Cite this article: Bone Jt Open 2023;4(11):825–831.
Acerca de la relación entre el gasto público por alumno y los retornos a la educación en el Perú: un análisis por cohortes
Authors: García Núñez Luis , Sánchez Romero Sara
Published: November 2023

Between 1960 and 2015, real public spending on education per student in Peru witnessed a significant decline at all levels until 1990, followed by a moderate recovery. Economic theory relates this expenditure to the quality of education, which can directly affect private returns to education in the labor market. The objective of this research is to find this relationship using econometric methods and household surveys. Using a cohort approach based on the methodology introduced by Deaton and Paxson (1993) and Attanasio (1993), we uncover a declining trend in returns for older generations with a modest recovery for more recent generations, especially for higher education and for female workers. Furthermore, we also discuss the importance of bolstering public spending and enhancing education quality, emphasizing the need for this dual approach.
How is climate change adaptation aid allocated? A study of climate justice in Ecuador
Authors: Cisneros Paul , Ilbay-Yupa Mercy
Published: November 2023

This paper analyses the allocation of climate adaptation aid in subnational and local jurisdictions in Ecuador. It examines the influence of physical and socioeconomic vulnerability and local capacity as independent variables of aid allocation. The study introduces projections of physical vulnerability to determine whether inter-generational aspects of climate justice are taken into account and uses geographically weighted regression models to study the spatial distribution of aid allocation. The findings show that physical and socioeconomic vulnerabilities influence adaptation aid allocation. However, projected changes in patterns of precipitation and temperatures are not associated with this variable. Furthermore, the spatial distribution of current climate aid is uneven, leaving clusters of vulnerable jurisdictions unattended. Finally, the study finds no association between local capacity and adaptation aid allocation. Findings suggest that the allocation of adaptation aid may not contribute to achieving adaptation scenarios where justice for current and future generations is central to decision-making.
Use of Social Representation to Validate the Structural Equations Modelling
Authors: Marchisotti Gustavo Guimarães , Rodrigues de Farias Filhos José , Luiz Braga França Sérgio , Cristiano Gomes Alves de Castro Hélio , Bayma de Oliveira Fátima
Published: November 2023

This article proposes the use of social representation theory to validate the structural model of structural equation modeling and better understand the research object. For this purpose, action research was used to construct, implement, and confirm the practical feasibility of adopting the methodological procedures described in this article, based on their practical application in an analysis case. We concluded that it was possible to validate the structural model used in structural equation modeling, using the proposed methodological procedures, applied to a case involving the governance system construct. This opens up the possibility of its future use in other research, which use structural equation modeling to validate a theoretical model and the causal relationships between its constructs. As such, we reduce the risk of using this statistical method to validate a theoretical model whose causal relationships do not represent a reality supported by practice, going beyond theoretical validation.
Atypical orbital primary optic nerve sheath meningioma with severe disfiguring proptosis: an alternative surgical approach
Authors: Patrick Sylves , Tat Yeap Boon , Amin Muhamad Zarif Mohd , Addenan Maftuhim , Ab.Ghani Shuaibah , Hanafi Hanida
Published: November 2023

Primary optic nerve sheath meningioma is generally a benign tumor. In rare instances, however, the growth rate and intraocular and intracranial extensions can be highly aggressive, especially in children, leading to poor prognosis. Here, we reported a case of a 24-year-old woman who presented with left eye swelling for 3 years. This was associated with blurred vision, retrobulbar pain, and redness. On examination, the left eye was severely proptosed with complete ophthalmoplegia. Magnetic resonance imaging showed an extensive tumor occupying the whole left orbital cavity with a disfigured eyeball. However, no intracranial extension was observed. Interestingly, complete surgical excision was feasible via transconjunctival anterior orbitotomy without bone removal. The histopathological examination confirmed the diagnosis of optic nerve sheath meningioma. Adjunct radiotherapy was given. On a follow-up after 2 years, left enophthalmos with esotropia was observed.
CTDNet: cartoon-texture decomposition-based gray image super-resolution network with multiple degradations
Authors: Shi Baoshun , Xu Wenyuan , Yang Xiuwei
Published: November 2023

In the case of multiple degradations, current deep-learning-based gray image super-resolution (SR) methods equally process all components in an image, resulting in missing subtle details. To address this issue, we elaborate a cartoon-texture decomposition-based (CTD) module that can automatically decompose an image into a smooth cartoon component and an oscillatory texture component. The CTD module is a plug-and-play prior module that can be applied in solving imaging inverse problems. Specifically, for the SR task under multiple degradations, we apply CTD as a prior module to build an unfolding SR network termed CTDNet. For the SR task of real terahertz images, the boundary (i.e., the boundary between the object of interest and the carrier table) recovered by CTDNet has artifacts, which limits its realistic applications. To reduce these boundary artifacts, we post-process the SR terahertz images by using a boundary artifact reduction method. Experimental results on the synthetic dataset and real terahertz images demonstrate that the proposed algorithms can maintain subtle details and achieve comparable visual results. The code can be found at https://github.com/shibaoshun/CTDNet.
Efficient continuous-wave Nd:YVO4/KGW intracavity Raman laser
Authors: Geng Jingni , Sheng Quan , Fu Shijie , Shi Wei , Yao Jianquan
Published: November 2023

We demonstrate an efficient Nd:YVO4/KGW intracavity Raman laser in continuous-wave (CW) scheme. With a V-shaped fundamental laser cavity and a short Stokes cavity in it, the oscillating beam sizes are designed to alleviate the thermal effect and to enhance the Raman gain for efficient CW operation. The output power of CW Stokes wave at 1177 nm reached 9.33 W under an incident laser diode pump power of 36.65 W, with corresponding optical efficiency being 25.5%. To the best of our knowledge, these are the highest Stokes output power and conversion efficiency of CW intracavity Raman lasers.
Broadband optical properties of Ti3C2 MXene revisited
Authors: Panova Daria A. , Tselikov Gleb I. , Ermolaev Georgy A. , Syuy Alexander V. , Zimbovskii Dmitrii S. , Kapitanova Olesya O. , Yakubovsky Dmitry I. , Mazitov Arslan B. , Kruglov Ivan A. , Vyshnevyy Andrey A. , Arsenin Aleksey V. , Volkov Valentyn S.
Published: November 2023

The exceptional optical, electrical, and mechanical capabilities of layered transition metal carbides, nitrides, and carbonitrides, called MXenes, revolutionized materials science. Among them, Ti3C2 received the most attention owing to the developed synthesis and processing methods, high conductivity, and pronounced plasmonic response. The latter, however, remains controversial with the open question of whether the peak around 800 nm has plasmonic or interband transition origin. To address this issue, we combine spectroscopic ellipsometry and transmittance results with first-principle computations. Their combination reveals that although Ti3C2 is a metal, its optical response becomes plasmonic (Re ε < 0) above 1415 nm, in contrast to the previous understanding. In addition to fundamental significance, this dual dielectric/plasmonic optical response opens a path for theranostic applications, as we demonstrated on the example of Ti3C2 nanospheres. Thus, our study revisits broadband (300–3300 nm) optical constants of Ti3C2 and broadens its application scope in photonics.
High-power narrow spectral width 975 nm semiconductor laser with high process compatibility achieved by high-order gratings
Authors: Liu Zhenwu , Zhong Li , Li Wei , Liu Suping , Ma Xiaoyu
Published: November 2023

We present broad-area semiconductor lasers with a 100th-order high-order distributed Bragg reflection (HO-DBR) grating structure, fabricated with the conventional UV lithography techniques. Based on the finite-difference time-domain (FDTD) algorithm, a maximum output power of 10.5 W, emitting around 975 nm with a spectral width of less than 0.5 nm FWHM has been achieved. This method provides insights for reducing the manufacturing costs of the high-power narrow spectral width DBR lasers.
Observation of higher-order topological corner states in photonic two-dimensional trimer lattices
Authors: Cheng Weizhao , Liu Weijie , Yan Wenchao , Zhang Bin , Chen Feng
Published: November 2023

We demonstrate the first, to the best of our knowledge, experimental observation of higher-order topological corner states in the photonic two-dimensional (2D) trimer lattices. Using a femtosecond laser direct writing technology, we experimentally fabricate a series of 2D trimer lattices with different open boundary conditions and thereby observe two kinds of 0D topological corner states, i.e., topological corner states and topological defect corner states. Interestingly, these corner states and defect corner states can not only exist in the bandgap but also coexist with the bulk states and show obvious localization properties. This work provides fresh perspectives on higher-order topology in artificial microstructures.
Sexual and reproductive health among adolescents in vulnerable contexts in Mexico: Needs, knowledge, and rights
Authors: Villalobos Aremis , Estrada Fátima , Hubert Celia , Torres-Ibarra Leticia , Rodríguez Alejandro , Romero Irma , Schiavon Raffaela , Campero Lourdes
Published: November 2023

Ensuring sexual and reproductive health, and rights for adolescents entails the prevention of early pregnancies, which are widely recognized as a public health problem. Based on the ecological model for early pregnancy, this article identifies the healthcare requirements for preventing unintended adolescent pregnancies in predominantly indigenous communities in Chiapas, Mexico. Using a convergent parallel mixed-methods study design, we surveyed adolescents (12–15 years old) and health personnel, organized focus groups with adolescents and their parents, and conducted in-depth interviews at the individual, family, school and community levels. Results showed that adolescents recognized their right to receive sexuality education (64.5%) as well as information on contraceptive methods (53.0%), with indigenous language speakers and individuals living in overcrowded households less likely to know about these rights. Parents of adolescents knew little about contraception and pregnancy. School teachers lacked necessary tools for offering comprehensive sexuality education. A traditional, patriarchal perspective predominated among participants, fostering gender inequalities. In conclusion, it is essential to implement multifocal strategies under a human-rights, intercultural, and health-equity approach. Special attention should be directed to the spheres in which adolescents interact, and efforts should focus on improving knowledge, empowering adolescents, and enhancing their access to sexual and reproductive health resources.
Developments in Leishmaniasis diagnosis: A patent landscape from 2010 to 2022
Authors: de Avelar Daniel Moreira , Santos Camila Chaves , Fusaro Faioli Alice
Published: November 2023

The current study aims to contribute to the understanding of leishmaniasis diagnosis by providing an overview of patent filings in this field and analyzing whether the methods revealed are consistent with the needs described by the scientific community, in special the main gaps detected by the World Health Organization’s 2021–2030 Roadmap for Neglected Tropical Diseases. To this aim, a patent search was carried out focusing on documents disclosing leishmaniasis diagnostic methods supported by experimental evidence and with earliest priority date from 2010 onwards. Our results show that patenting activity is low and patent families are often formed by individual filings. Most R&D activity occurs in Brazil, which is also the main market of protection. Brazilian academic institutions are the main patent drivers, and collaboration between different institutions is rare. Most patent families describe immunological methods based on ELISA assays, using antibodies directed to K39 and homologues. kDNA is the primary gene for molecular testing. Experimental evidence of test performance in fulfilling critical diagnostic gaps is usually absent. The patent scenario suggests that leishmaniasis diagnostic gaps need to be more closely addressed to drive innovation directed to the control and/or elimination of leishmaniasis. From the public policy point of view, the following strategies are suggested: (i) strengthening collaborative networks, (ii) enhancing the participation of the private sector, and (iii) increasing funding, with special focus on the remaining diagnostic gaps.
Tumor necrosis factor-α (TNF-α) -308G >a promoter polymorphism (rs1800629) promotes Asians in susceptibility to Plasmodium falciparum severe malaria: A meta-analysis
Authors: Kongjam Panida , Pabalan Noel , Tharabenjasin Phuntila , Jarjanazi Hamdi , Chaijaroenkul Wanna , Na-Bangchang Kesara
Published: November 2023

The multifactorial pathogenesis of severe malaria is partly attributed to host genes, such as those encoding cytokines involved in complex inflammatory reactions, namely tumor necrosis factor-alpha (TNF-α). However, the relationship between TNF-α -308G >A gene polymorphism (rs1800629) and the severity of Plasmodium falciparum (P. falciparum) malaria remains unclear, which prompts a meta-analysis to obtain more precise estimates. The present meta-analysis aimed to better understand this correlation and provide insight into its association in populations with different ethnicities. Literature search outcomes included eight eligible articles in which TNF-α -308G >A polymorphism was determined in uncomplicated malaria (UM) and severe malaria (SM) of P. falciparum as represented in the case and control groups. Pooled odds ratios (ORs) and 95% confidence intervals (95% CIs) were estimated in standard homozygous, recessive, dominant, and codominant genetic models. Subgroup analysis was based on ethnicity, i.e., Africans and Asians. The analyses included overall and the modified outcomes; the latter was obtained without the studies that deviated from the Hardy-Weinberg Equilibrium. The significant data also underwent sensitivity treatment but not publication bias tests because the number of studies was less than ten. Interaction tests were applied to differential outcomes between the subgroups. Overall and HWE-compliant analyses showed no significant association between the TNF-α -308G >A polymorphism and susceptibility to P. falciparum SM (ORs = 1.10–1.52, 95%CIs = 0.68–2.79; Pa = 0.24–0.68). Stratification by ethnicity revealed that two significant associations were found only in the Asians favoring SM for dominant (OR = 1.95, 95% CI = 1.06–3.61, Pa = 0.03) and codominant (OR = 1.83, 95% CI = 1.15–2.92, Pa = 0.01) under the random-effects model, but not among the African populations. The two significant Asian associations were improved with a test of interaction with P-value of0.02–0.03. The significant core outcomes were robust. Results of the meta-analysis suggest that TNF-α -308G >A polymorphism might affect the risk of P. falciparum SM, particularly in individuals of Asian descent. This supports ethnicity as one of the dependent factors of the TNF-α -308G >A association with the clinical severity of malaria. Further large and well-designed genetic studies are needed to confirm this conclusion.
Screening fructosamine-3-kinase (FN3K) inhibitors, a deglycating enzyme of oncogenic Nrf2: Human FN3K homology modelling, docking and molecular dynamics simulations
Authors: Beeraka Narasimha M. , Zhang Jin , Mandal Subhankar , Vikram P. R. Hemanth , Liu Junqi , B. M. Namitha , Zhao Di , Vishwanath Prashanth , B. M. Gurupadayya , Fan Ruitai
Published: November 2023

Fructosamine-3-kinase (FN3K) is involved in the deglycation of Nrf2, a significant regulator of oxidative stress in cancer cells. However, the intricate functional aspects of FN3K and Nrf2 in breast cancers have not been explored vividly. The objectives of this study are to design the human FN3K protein using homology modeling followed by the screening of several anticancer molecules and examining their efficacy to modulate FN3K activity, Nrf2-mediated antioxidant signalling. Methods pertinent to homology modeling, virtual screening, molecular docking, molecular dynamics simulations, assessment of ADME properties, cytotoxicity assays for anticancer molecules of natural/synthetic origin in breast cancer cells (BT-474, T-47D), and Western blotting were used in this study. The screened anticancer molecules including kinase inhibitors of natural and synthetic origin interacted with the 3-dimensional structure of the catalytic domain in human FN3K protein designed through homology modeling by significant CDOCKER interaction energies. Subsequently, gefitinib, sorafenib, neratinib, tamoxifen citrate, and cyclosporine A enhanced the expression of FN3K in BT-474 cell lines with simultaneous alteration in Nrf2-driven antioxidant signalling. Oxaliplatin significantly downregulated FN3K expression and modulated Nrf2-driven antioxidant signalling when compared to cisplatin and other anticancer drugs. Hence, the study concluded the potential implications of existing anticancer drugs to modulate FN3K activity in breast cancers.
Emergency department visits and boarding for pediatric patients with suicidality before and during the COVID-19 pandemic
Authors: Zipursky Amy R. , Olson Karen L. , Bode Louisa , Geva Alon , Jones James , Mandl Kenneth D. , McMurry Andrew
Published: November 2023

Objective

To quantify the increase in pediatric patients presenting to the emergency department with suicidality before and during the COVID-19 pandemic, and the subsequent impact on emergency department length of stay and boarding.

Methods

This retrospective cohort study from June 1, 2016, to October 31, 2022, identified patients ages 6 to 21 presenting to the emergency department at a pediatric academic medical center with suicidality using ICD-10 codes. Number of emergency department encounters for suicidality, demographic characteristics of patients with suicidality, and emergency department length of stay were compared before and during the COVID-19 pandemic. Unobserved components models were used to describe monthly counts of emergency department encounters for suicidality.

Results

There were 179,736 patient encounters to the emergency department during the study period, 6,215 (3.5%) for suicidality. There were, on average, more encounters for suicidality each month during the COVID-19 pandemic than before the COVID-19 pandemic. A time series unobserved components model demonstrated a temporary drop of 32.7 encounters for suicidality in April and May of 2020 (p<0.001), followed by a sustained increase of 31.2 encounters starting in July 2020 (p = 0.003). The average length of stay for patients that boarded in the emergency department with a diagnosis of suicidality was 37.4 hours longer during the COVID-19 pandemic compared to before the COVID-19 pandemic (p<0.001).

Conclusions

The number of encounters for suicidality among pediatric patients and the emergency department length of stay for psychiatry boarders has increased during the COVID-19 pandemic. There is a need for acute care mental health services and solutions to emergency department capacity issues.
Dehydration alters behavioral thermoregulation and the geography of climatic vulnerability in two Amazonian lizards
Authors: Camacho Agustín , Brunes Tuliana O. , Rodrigues Miguel Trefaut
Published: November 2023

High temperatures and low water availability often strike organisms concomitantly. Observing how organisms behaviorally thermohydroregulate may help us to better understand their climatic vulnerability. This is especially important for tropical forest lizards, species that are purportedly under greater climatic risk. Here, we observed the influence of hydration level on the Voluntary Thermal Maximum (VTmax) in two small Amazonian lizard species: Loxopholis ferreirai (semiaquatic and scansorial) and Loxopholis percarinatum (leaf litter parthenogenetic dweller), accounting for several potential confounding factors (handling, body mass, starting temperature and heating rate). Next, we used two modeling approaches (simple mapping of thermal margins and NicheMapR) to compare the effects of dehydration, decrease in precipitation, ability to burrow, and tree cover availability, on geographic models of climatic vulnerability. We found that VTmax decreased with dehydration, starting temperature, and heating rates in both species. The two modeling approaches showed that dehydration may alter the expected intensity, extent, and duration of perceived thermal risk across the Amazon basin for these forest lizards. Based on our results and previous studies, we identify new evidence needed to better understand thermohydroregulation and to model the geography of climatic risk using the VTmax.
Interleukin-27-induced HIV-resistant dendritic cells suppress reveres transcription following virus entry in an SPTBN1, autophagy, and YB-1 independent manner
Authors: Imamichi Tomozumi , Chen Qian , Sowrirajan Bharatwaj , Yang Jun , Laverdure Sylvain , Marquez Mayra , Mele Anthony R. , Watkins Catherine , Adelsberger Joseph W. , Higgins Jeanette , Sui Hongyan
Published: November 2023

Interleukin (IL)-27, a member of the IL-12 family of cytokines, induces human immunodeficiency virus (HIV)-resistant monocyte-derived macrophages and T cells. This resistance is mediated via the downregulation of spectrin beta, non-erythrocytic 1 (SPTBN1), induction of autophagy, or suppression of the acetylation of Y-box binding protein-1 (YB-1); however, the role of IL-27 administration during the induction of immature monocyte-derived dendritic cells (iDC) is poorly investigated. In the current study, we investigated the function of IL-27-induced iDC (27DC) on HIV infection. 27DC inhibited HIV infection by 95 ± 3% without significant changes in the expression of CD4, CCR5, and SPTBN1 expression, autophagy induction and acetylation of YB-1 compared to iDC. An HIV proviral DNA copy number assay displayed that 27DC suppressed reverse transcriptase (RT) reaction without influencing the virus entry. A DNA microarray analysis was performed to identify the differentially expressed genes between 27DC and iDC. Compared to iDC, 51 genes were differentially expressed in 27DC, with more than 3-fold changes in four independent donors. Cross-reference analysis with the reported 2,214 HIV regulatory host genes identified nine genes as potential interests: Ankyrin repeat domain 22, Guanylate binding protein (GBP)-1, -2, -4, -5, Stabilin 1, Serpin family G member 1 (SERPING1), Interferon alpha inducible protein 6, and Interferon-induced protein with tetratricopeptide repeats 3. A knock-down study using si-RNA failed to determine a key factor associated with the anti-HIV activity due to the induction of robust amounts of off-target effects. Overexpression of each protein in cells had no impact on HIV infection. Thus, we could not define the mechanism of the anti-HIV effect in 27DC. However, our findings indicated that IL-27 differentiates monocytes into HIV-resistant DC, and the inhibitory mechanism differs from IL-27-induced HIV-resistant macrophages and T cells.
A comparison of presentations and outcomes of salvage versus non-salvage abdominal free flap breast reconstructions—Results of a 15-year tertiary referral centre review
Authors: Bojanic Christine , Di Pace Bruno , Ghorra Dina T. , Fopp Laura J. , Rabey Nicholas G. , Malata Charles M.
Published: November 2023

Introduction

Salvage breast reconstruction with autologous tissue is becoming more prevalent due to a resurgence in implant-based procedures. The latter has caused a commensurate rise in failed or treatment-resistant prosthetic cases requiring conversion to free tissue transfers. Salvage reconstruction is often considered more challenging, owing to patient presentation, prior treatments and intraoperative difficulties. The aim of the study was to test this hypothesis by comparing outcomes of salvage versus non-salvage autologous microsurgical breast reconstructions in a retrospective matched cohort study.

Methods

The demographics, risk factors, operative details and outcomes of patients who underwent free flap salvage of implant-based reconstructions by a single operator (2005–2019) were retrospectively evaluated. For each salvage reconstruction, the consecutive non-salvage abdominal free flap reconstruction was selected for comparison. The clinical outcomes including intraoperative blood loss, operative time, flap survival and complication rates were compared.

Results

Of 442 microsurgical patients, 35 (8.0%) had salvage reconstruction comprising 41 flap transfers (29 unilateral, 6 bilateral) and 42 flaps (28 unilateral, 7 bilateral) in nonsalvage reconstruction. Deep inferior epigastric perforator (DIEP) flaps comprised the commonest autologous tissue used in both groups at 74% and 71% respectively. Most patients (83%) underwent salvage reconstruction for severe capsular contractures. There was a significant difference in radiation exposure between groups (salvage reconstruction 89%, non-salvage reconstruction 26%; p<0.00001). All 83 flaps were successful with similar reoperation rates and intraoperative blood losses. Unilateral salvage reconstruction took on average two hours longer than non-salvage reconstruction (p = 0.008). Overall complication rates were similar (p>0.05).

Conclusion

This 15-year study shows that despite salvage autologous free flap breast reconstruction requiring longer operation times, its intra and postoperative outcomes are generally comparable to non-salvage cases. Therefore, salvage breast reconstruction with free flaps provides a reliable option for failed or suboptimal implant-based reconstructions.
Biophilic classroom environments on stress and cognitive performance: A randomized crossover study in virtual reality (VR)
Authors: You Jicheng , Wen Xinyi , Liu Linxin , Yin Jie , Ji John S.
Published: November 2023

The emerging Metaverse will likely increase time expenditure in indoor virtual environments, which could impact human health and well-being. The biophilia hypothesis suggests that humans have an innate tendency to seek connections with the natural world and there is increasing evidence that biophilic design such as the incorporation of green plants can yield health benefits. Recently, virtual reality (VR) has been used to regulate stress and improve overall wellness, particularly by incorporating natural settings. In this randomized crossover study, we designed five virtual classroom scenes with different biophilic elements and turbidity in VR and investigated whether the visual stimulations can affect the stress levels and cognitive functions of 30 young adults from a university in China. We measured their physiological indicators of stress reaction by wearable biomonitoring sensors (blood pressure (BP), heart rate (HR), heart rate variability (HRV), and skin conductance level (SCL)), conducted verbal cognitive tests on attention and creativity, and evaluated subjective/perceived (self-reported) stress levels and connection with nature. Albeit our results suggested no significant change in physiological stress reactions or cognitive functions induced by the biophilic and turbid interventions in VR, the addition of biophilic elements in the Metaverse could benefit students’ health due to significantly decreased perceived stress levels and increased connections with nature.
A simple and efficient protocol for generating transgenic hairy roots using Agrobacterium rhizogenes
Authors: Ferguson Shaun , Abel Nikolaj B. , Reid Dugald , Madsen Lene H. , Luu Thi-Bich , Andersen Kasper R. , Stougaard Jens , Radutoiu Simona
Published: November 2023

For decades, Agrobacterium rhizogenes (now Rhizobium rhizogenes), the causative agent of hairy root disease, has been harnessed as an interkingdom DNA delivery tool for generating transgenic hairy roots on a wide variety of plants. One of the strategies involves the construction of transconjugant R. rhizogenes by transferring gene(s) of interest into previously constructed R. rhizogenes pBR322 acceptor strains; little has been done, however, to improve upon this system since its implementation. We developed a simplified method utilising bi-parental mating in conjunction with effective counterselection for generating R. rhizogenes transconjugants. Central to this was the construction of a new Modular Cloning (MoClo) compatible pBR322-derived integration vector (pIV101). Although this protocol remains limited to pBR322 acceptor strains, pIV101 facilitated an efficient construction of recombinant vectors, effective screening of transconjugants, and RP4-based mobilisation compatibility that enabled simplified conjugal transfer. Transconjugants from this system were tested on Lotus japonicus and found to be efficient for the transformation of transgenic hairy roots and supported infection of nodules by a rhizobia symbiont. The expedited protocol detailed herein substantially decreased both the time and labour for creating transconjugant R. rhizogenes for the subsequent transgenic hairy root transformation of Lotus, and it could readily be applied for the transformation of other plants.
Mediolateral foot placement control can be trained: Older adults learn to walk more stable, when ankle moments are constrained
Authors: Mahaki Mohammadreza , van Leeuwen Anina Moira , Bruijn Sjoerd M. , van der Velde Nathalie , van Dieën Jaap H.
Published: November 2023

Falls are a problem, especially for older adults. Placing our feet accurately relative to the center-of-mass helps us to prevent falling during gait. The degree of foot placement control with respect to the center-of mass kinematic state is decreased in older as compared to young adults. Here, we attempted to train mediolateral foot placement control in healthy older adults. Ten older adults trained by walking on shoes with a narrow ridge underneath (LesSchuh), restricting mediolateral center-of-pressure shifts. As a training effect, we expected improved foot placement control during normal walking. A training session consisted of a normal walking condition, followed by a training condition on LesSchuh and finally an after-effect condition. Participants performed six of such training sessions, spread across three weeks. As a control, before the first training session, we included two similar sessions, but on normal shoes only. We evaluated whether a training effect was observed across sessions and weeks in a repeated-measures design. Whilst walking with LesSchuh, the magnitude of foot placement error reduced half-a-millimeter between sessions within a week (cohen’s d = 0.394). As a training effect in normal walking, the magnitude of foot placement errors was significantly lower compared to the control week, by one millimeter in weeks 2 (cohen’s d = 0.686) and 3 (cohen’s d = 0.780) and by two millimeters in week 4 (cohen’s d = 0.875). Local dynamic stability of normal walking also improved significantly. More precise foot placement may thus have led to improved stability. It remains to be determined whether the training effects were the result of walking on LesSchuh or from repeated treadmill walking itself. Moreover, enhancement of mechanisms beyond the scope of our outcome measures may have improved stability. At the retention test, gait stability returned to similar levels as in the control week. Yet, a reduction in foot placement error persisted.
Word differences in news media of lower and higher peace countries revealed by natural language processing and machine learning
Authors: Liebovitch Larry S. , Powers William , Shi Lin , Chen-Carrel Allegra , Loustaunau Philippe , Coleman Peter T.
Published: November 2023

Language is both a cause and a consequence of the social processes that lead to conflict or peace. “Hate speech” can mobilize violence and destruction. What are the characteristics of “peace speech” that reflect and support the social processes that maintain peace? This study used existing peace indices, machine learning, and on-line, news media sources to identify the words most associated with lower-peace versus higher-peace countries. As each peace index measures different social properties, they can have different values for the same country. There is however greater consensus with these indices for the countries that are at the extremes of lower-peace and higher-peace. Therefore, a data driven approach was used to find the words most important in distinguishing lower-peace and higher-peace countries. Rather than assuming a theoretical framework that predicts which words are more likely in lower-peace and higher-peace countries, and then searching for those words in news media, in this study, natural language processing and machine learning were used to identify the words that most accurately classified a country as lower-peace or higher-peace. Once the machine learning model was trained on the word frequencies from the extreme lower-peace and higher-peace countries, that model was also used to compute a quantitative peace index for these and other intermediate-peace countries. The model successfully yielded a quantitative peace index for intermediate-peace countries that was in between that of the lower-peace and higher-peace, even though they were not in the training set. This study demonstrates how natural language processing and machine learning can help to generate new quantitative measures of social systems, which in this study, were linguistic differences resulting in a quantitative index of peace for countries at different levels of peacefulness.
The impact of pet ownership on healthcare access and utilization among people with HIV
Authors: Applebaum Jennifer W. , McDonald Shelby E. , Widmeyer Maya , Fabelo Humberto E. , Cook Robert L.
Published: November 2023

Though bonds with pets can be health-promoting for people with HIV (PWH), recent studies indicate that owning pets may complicate healthcare access, especially for those with fewer economic resources, poorer social support, and a strong human-animal bond. In this study, we make a case for considering pets to be an important element of the social environment that can influence healthcare access and utilization among PWH. Pet-owning PWH (n = 204) were recruited at healthcare and community sites throughout Florida as part of a larger survey study (the “Florida Cohort”). We developed a 12-item index of pet-related barriers to healthcare, which was designed to assess whether the participants experienced or anticipated any barriers to accessing and/or utilizing timely healthcare or health-related services due to pet caregiving or concerns about pet welfare. We estimated a series of regression models (negative binomial, logistic regression) to assess the effects of comfort from companion animals, human social support, healthcare needs, and sociodemographic characteristics on 1) the total number of pet-related healthcare barriers endorsed, 2) previously experienced pet-related healthcare barriers, and 3) anticipated pet-related healthcare barriers. Thirty-six percent of the sample reported at least one experienced or anticipated pet-related barrier to their healthcare; 17% reported previous healthcare barriers and 31% anticipated future healthcare barriers. Greater comfort from companion animals, greater healthcare needs, and poorer social support were associated with a greater probability of experiencing or anticipating any pet-related healthcare barriers. Those who identified racially as Black were less likely to anticipate future healthcare barriers than those who were White. Income was associated with pet-related healthcare barriers in all models. Given the importance of health maintenance for PWH and previous research suggesting pets may be an important emotional support for this population, social safety net programs and community partnerships that support multispecies families are strongly recommended.
Numerical study of the tire hydroplaning behavior of aircraft on grooved concrete pavement
Authors: Cai Jing , Du Nizhi , Zhou Ning , Li Yue , Dai Xuan , Zhang Heng
Published: November 2023

Safe operation is crucial for civil aviation, and reducing the risk of aircraft tire hydroplaning is essential for civil aviation safety. Here, a new 3D aircraft tire-grooved (smooth) wet pavement model based on the coupled Eulerian-Lagrangian (CEL) algorithm for the A320 aircraft was developed, and the effect of the ground contact area of an aircraft tire on the hydrodynamic pressure and support force of the tire under smooth and grooved wet pavement conditions was investigated. The results indicate that at the same taxiing speed, the ground contact area of the aircraft tire under the grooved wet-pavement condition is reduced by 19.8% compared to the smoothed wet-pavement condition, which is reduced by 6.2%. Similar patterns are observed for the hydrodynamic pressure and the critical hydrodynamic speed during landing and taking-off procedures, with upper and lower limited values obtained through the simulation results. Additionally, the predicted correction factor of the hydroplaning speed at different water film thicknesses is compared with those values obtained via the NASA formula. A comparison shows that the NASA formula underestimates the critical hydroplaning speed during the landing procedure. The corresponding correction factor will be less than 1.0 when the water film thickness reaches a critical value of 7.66 mm.
Evaluation of lay health workers on quality of care in the inpatient setting
Authors: Basnight Ramona , Berry Peter , Capes Kellie , Pearce Sherri , Thompson Julie , Allen Deborah H. , Granger Bradi B. , Reynolds Staci S.
Published: November 2023

Aims

To evaluate the impact of a lay health worker support role in the inpatient setting.

Background

Healthcare systems are facing critical nursing and nurse assistant staffing shortages. These disciplines can be challenging to recruit and retain, leading healthcare leaders to identify innovative staffing models. Whereas lay health workers have been used in the community and low-income setting, there is scant evidence of their use in the inpatient setting. We implemented a lay health worker role, called Patient Attendant Service Aides (PASAs), on two medical/surgical units at a community hospital.

Methods

A pre/post-implementation design was used for this study. An online survey was provided to nurses, nursing assistants, and PASAs on the two medical/surgical units to assess their satisfaction and perceptions of the role. Nursing quality metrics, patient satisfaction, and nursing and nursing assistant turnover were evaluated before and after implementing the role.

Results

The online survey showed that nurses and nursing assistants felt that PASAs helped offload their workload, allowing them to focus on nursing-related tasks. PASAs felt supported by the team and believed they were making a meaningful contribution to the unit. There were slight improvements in patient satisfaction, although not significant. There was a significant improvement in nursing turnover on Unit A, from 71.1% to 21.6% (p = 0.009).

Conclusions

This is one of the first studies to evaluate the use of lay health workers in the inpatient setting; we found this role to be a feasible way to offload tasks from clinical staff. This role may serve as a pathway for workforce development, as several PASAs are now enrolled in nursing assistant training. Nurse managers may consider using lay health workers in the inpatient setting as they face severe clinical staff shortages.
Pangenomic antiviral effect of REP 2139 in CRISPR/Cas9 engineered cell lines expressing hepatitis B virus surface antigen
Authors: Angelo Léna , Vaillant Andrew , Blanchet Matthieu , Labonté Patrick
Published: November 2023

Chronic hepatitis B remains a global health problem with 296 million people living with chronic HBV infection and being at risk of developing cirrhosis and hepatocellular carcinoma. Non-infectious subviral particles (SVP) are produced in large excess over infectious Dane particles in patients and are the major source of Hepatitis B surface antigen (HBsAg). They are thought to exhaust the immune system, and it is generally considered that functional cure requires the clearance of HBsAg from blood of patient. Nucleic acid polymers (NAPs) antiviral activity lead to the inhibition of HBsAg release, resulting in rapid clearance of HBsAg from circulation in vivo. However, their efficacy has only been demonstrated in limited genotypes in small scale clinical trials. HBV exists as nine main genotypes (A to I). In this study, the HBsAg ORFs from the most prevalent genotypes (A, B, C, D, E, G), which account for over 96% of human cases, were inserted into the AAVS1 safe-harbor of HepG2 cells using CRISPR/Cas9 knock-in. A cell line producing the D144A vaccine escape mutant was also engineered. The secretion of HBsAg was confirmed into these new genotype cell lines (GCLs) and the antiviral activity of the NAP REP 2139 was then assessed. The results demonstrate that REP 2139 exerts an antiviral effect in all genotypes and serotypes tested in this study, including the vaccine escape mutant, suggesting a pangenomic effect of the NAPs.
Sexual health literacy and its related factors among couples: A population-based study in Iran
Authors: Shahrahmani Hadis , Kariman Nourossadat , Keshavarz Zohreh , Ahmadi Atefeh , Nasiri Malihe
Published: November 2023

Sexual health literacy is one of the factors that affect sexual health. Several factors can influence sexual health literacy. As a result, the current study was carried out to determine sexual health literacy and its associated factors among Iranian couples. In 2022, 410 couples of reproductive age were referred to comprehensive health service centers and private clinics in Kerman city for the descriptive-analytical study. The study questionnaires included sexual health literacy for adults, sexual knowledge and attitude scale, marital intimacy scale, sexual intimacy scale, depression anxiety stress scale, sexual self-efficacy, the multidimensional scale of perceived social support, and socioeconomic status. Finally, multiple linear stepwise regression models were used to determine the factors related to sexual health literacy using the SPSS software version 22. According to the findings of this study, the mean(SD) of sexual health literacy in couples was 68.76(12.96), which was a desirable level. Furthermore, the findings revealed that sexual self-efficacy (B = 0.649، P<0.001), sexual knowledge and attitude (B = 0.217، P<0.001), the ability to identify reliable and non-reliable sources (B = -3.116، P<0.001), feelings of shame and embarrassment for obtaining sexual information (B = 1.860، P = 0.011), social support (B = 0.127، P<0.001) and the obscenity of sexual issues in family (B = 1.764، P = 0.015) were the final predictors of sexual health literacy in couples. It is suggested that researchers and health managers consider these factors when designing interventions to promote sexual health literacy.
CD11c is not required by microglia to convey neuroprotection after prion infection
Authors: Carroll James A. , Striebel James F. , Baune Chase , Chesebro Bruce , Race Brent
Published: November 2023

Prion diseases are caused by the misfolding of a normal host protein that leads to gliosis, neuroinflammation, neurodegeneration, and death. Microglia have been shown to be critical for neuroprotection during prion infection of the central nervous system (CNS), and their presence extends survival in mice. How microglia impart these benefits to the infected host are unknown. Previous transcriptomics and bioinformatics studies suggested that signaling through the heterodimeric integrin receptor CD11c/CD18, expressed by microglia in the brain, might be important to microglial function during prion disease. Herein, we intracerebrally challenged CD11c-/- mice with prion strain RML and compared them to similarly infected C57BL/6 mice as controls. We initially assessed changes in the brain that are associated with disease such as astrogliosis, microgliosis, prion accumulation, and survival. Targeted qRT-PCR arrays were used to determine alterations in transcription in mice in response to prion infection. We demonstrate that expression of Itgax (CD11c) and Itgb2 (CD18) increases in the CNS in correlation with advancing prion infection. Gliosis, neuropathology, prion deposition, and disease progression in prion infected CD11c deficient mice were comparable to infected C57BL/6 mice. Additionally, both CD11c deficient and C57BL/6 prion-infected mouse cohorts had a similar consortium of inflammatory- and phagocytosis-associated genes that increased as disease progressed to clinical stages. Ingenuity Pathway Analysis of upregulated genes in infected C57BL/6 mice suggested numerous cell-surface transmembrane receptors signal through Spleen Tyrosine Kinase, a potential key regulator of phagocytosis and innate immune activation in the prion infected brain. Ultimately, the deletion of CD11c did not influence prion pathogenesis in mice and CD11c signaling is not involved in the neuroprotection provided by microglia, but our analysis identified a conspicuous phagocytosis pathway in the CNS of infected mice that appeared to be activated during prion pathogenesis.