bluuebunny/crossref_metadata_2025 huggingface dataset:
Transmission and Drive Involving Parasitic B Chromosomes
Authors: Jones R.N.
Published: August 2018

B chromosomes (Bs) are enigmatic additional elements in the genomes of thousands of species of plants, animals, and fungi. How do these non-essential, harmful, and parasitic chromosomes maintain their presence in their hosts, making demands on all the essential functions of their host genomes? The answer seems to be that they have mechanisms of drive which enable them to enhance their transmission rates by various processes of non-mendelian inheritance. It is also becoming increasingly clear that the host genomes are developing their own mechanisms to resist the impact of the harmful effects of the Bs.
Analysis of Candidate Idarubicin Drug Resistance Genes in MOLT-3 Cells Using Exome Nuclear DNA
Authors: Komiyama Tomoyoshi , Ogura Atsushi , Kajiwara Takehito , Okada Yoshinori , Kobayashi Hiroyuki
Published: August 2018

Various gene alterations related to acute leukemia are reported to be involved in drug resistance. We investigated idarubicin (IDR) resistance using exome nuclear DNA analyses of the human acute leukemia cell line MOLT-3 and the derived IDR-resistant cell line MOLT-3/IDR. We detected mutations in MOLT-3/IDR and MOLT-3 using both Genome Analysis Toolkit (GATK) and SnpEff program. We found 8839 genes with specific mutations in MOLT-3/IDR and 1162 genes with accompanying amino acid mutations. The 1162 genes were identified by exome analysis of polymerase-related genes using Kyoto Encyclopedia of Genes and Genomes (KEGG) and, among these, we identified genes with amino acid changes. In resistant strains, LIG and helicase plurality genes showed amino-acid-related changes. An amino acid mutation was also confirmed in polymerase-associated genes. Gene ontology (GO) enrichment testing was performed, and lipid-related genes were selected from the results. Fluorescent activated cell sorting (FACS) was used to determine whether IDR permeability was significantly different in MOLT-3/IDR and MOLT-3. The results showed that an IDR concentration of 0.5 μg/mL resulted in slow permeability in MOLT-3/IDR. This slow IDR permeability may be due to the effects of amino acid changes in polymerase- and lipid-associated genes.
A Model Stacking Framework for Identifying DNA Binding Proteins by Orchestrating Multi-View Features and Classifiers
Authors: Liu Xiu-Juan , Gong Xiu-Jun , Yu Hua , Xu Jia-Hui
Published: August 2018

Nowadays, various machine learning-based approaches using sequence information alone have been proposed for identifying DNA-binding proteins, which are crucial to many cellular processes, such as DNA replication, DNA repair and DNA modification. Among these methods, building a meaningful feature representation of the sequences and choosing an appropriate classifier are the most trivial tasks. Disclosing the significances and contributions of different feature spaces and classifiers to the final prediction is of the utmost importance, not only for the prediction performances, but also the practical clues of biological experiment designs. In this study, we propose a model stacking framework by orchestrating multi-view features and classifiers (MSFBinder) to investigate how to integrate and evaluate loosely-coupled models for predicting DNA-binding proteins. The framework integrates multi-view features including Local_DPP, 188D, Position-Specific Scoring Matrix (PSSM)_DWT and autocross-covariance of secondary structures(AC_Struc), which were extracted based on evolutionary information, sequence composition, physiochemical properties and predicted structural information, respectively. These features are fed into various loosely-coupled classifiers such as SVM and random forest. Then, a logistic regression model was applied to evaluate the contributions of these individual classifiers and to make the final prediction. When performing on the training dataset PDB1075, the proposed method achieves an accuracy of 83.53%. On the independent dataset PDB186, the method achieves an accuracy of 81.72%, which outperforms many existing methods. These results suggest that the framework is able to orchestrate various predicted models flexibly with good performances.
Postpartum Care Service Experience Model: A Case of Postpartum Home Visiting Services
Authors: Chang Pei-Ping
Published: August 2018

The choice of postpartum care methods has expanded with changes in population structure and social forms. In addition to the traditional provision of postpartum assistance by senior members of the parents’ family, recent years have also seen a rapid growth in care centers and postpartum home visiting services. Postpartum home visiting services combine the features and services of senior family members’ assistance and postpartum care centers. Therefore, special attention has been drawn to the postpartum care service experience. This study conducted a survey among mothers participating in the Postpartum Home Visiting Service Project of the Social Affairs Bureau of Kaohsiung City Government, Taiwan. The effective sample included 43 participants. Partial least squares (PLS) analysis was performed. The results indicated that the service experience model could comprehensively reflect the relation between consumer experience, experiential value, and experience satisfaction. With regard to practical implications, the results provide postpartum home visiting service providers with a better understanding of consumers’ feelings and, thus, put them in a better position to improve experience through appropriate service methods.
Changing Neighborhoods and Residents’ Health Perceptions: The Heart Healthy Hoods Qualitative Study
Authors: Conde Paloma , Gutiérrez Marta , Sandín María , Díez Julia , Borrell Luisa N. , Rivera-Navarro Jesús , Franco Manuel
Published: August 2018

Cities, and therefore neighborhoods, are under constant change. Neighborhood changes may affect residents’ health in multiple ways. The Heart Healthy Hoods (HHH) project studies the association between neighborhood and residents’ health. Focusing on a middle–low-socioeconomic neighborhood in Madrid (Spain), our aim was to describe qualitatively its residents’ perceptions on the urban changes and their impacts on health. We designed a qualitative study using 16 semi-structured interviews including adult residents and professionals living or working in the area. Firstly, we described the perceived main social and neighborhood changes. Secondly, we studied how these neighborhood changes connected to residents’ health perceptions. Perceived major social changes were new demographic composition, new socio–cultural values and economic changes. Residents’ negative health perceptions were the reduction of social relationships, increase of stress and labor precariousness. Positive health perceptions were the creation of supportive links, assimilation of self-care activities and the change in traditional roles. Neighborhood changes yielded both negative and positive effects on residents’ health. These effects would be the result of the interrelation of different elements such as the existence or absence of social ties, family responsibilities, time availability, economic resources and access and awareness to health-promoting programs. These qualitative research results provide important insight into crafting urban health policies that may ultimately improve health outcomes in communities undergoing change.
Mumps in the Vaccination Age: Global Epidemiology and the Situation in Germany
Authors: Beleni Andrea-Ioana , Borgmann Stefan
Published: August 2018

Vaccination against mumps virus (MuV) (mostly measles-mumps-rubella) is routinely performed in more than 120 countries and has resulted in a distinct decrease of mumps incidence. However, alteration of mumps epidemiology has been observed in several countries after implementation of the vaccine but is sparsely documented. Moreover, outbreaks have occurred after starting vaccination, even in highly vaccinated populations. In the former German Democratic Republic (DDR) mumps was a notifiable disease but vaccination against mumps was not implemented. In the five eastern German states forming the DDR until 1990, mumps was not notifiable until 2001. Except for the lack of reporting between 1990–2000, data from Eastern Germany allow analysis of mumps epidemiology after initiating the vaccination campaign. For the period from 2001 to 2016 the data show that the incidence of mumps dropped notably after initiating vaccines, and was accompanied by an increase of the median age of patients with mumps. In Eastern Germany, no outbreaks were noted, while several outbreaks occurred in Western Germany, possibly due to a lower vaccination rate. Further literature analysis revealed that outbreaks were facilitated by waning immunity and crowding. Nevertheless, although vaccination prevented infection, the course of illness, once infected, was sometimes more complicated. In comparison to non-vaccinated populations, high rates of complicated courses occurred and were marked by orchitis, due to higher age of mumps patients. Therefore, refusing vaccination against mumps increases the risk of severe courses when living in a vaccinated population.
A Closer Look at the Bivariate Association between Ambient Air Pollution and Allergic Diseases: The Role of Spatial Analysis
Authors: Kim Dohyeong , Seo SungChul , Min Soojin , Simoni Zachary , Kim Seunghyun , Kim Myoungkon
Published: August 2018

Although previous ecological studies investigating the association between air pollution and allergic diseases accounted for temporal or seasonal relationships, few studies address spatial non-stationarity or autocorrelation explicitly. Our objective was to examine bivariate correlation between outdoor air pollutants and the prevalence of allergic diseases, highlighting the limitation of a non-spatial correlation measure, and suggesting an alternative to address spatial autocorrelation. The 5-year prevalence data (2011–2015) of allergic rhinitis, atopic dermatitis, and asthma were integrated with the measures of four major air pollutants (SO2, NO2, CO, and PM10) for each of the 423 sub-districts of Seoul. Lee’s L statistics, which captures how much bivariate associations are spatially clustered, was calculated and compared with Pearson’s correlation coefficient for each pair of the air pollutants and allergic diseases. A series of maps showing spatiotemporal patterns of allergic diseases at the sub-district level reveals a substantial degree of spatial heterogeneity. A high spatial autocorrelation was observed for all pollutants and diseases, leading to significant dissimilarities between the two bivariate association measures. The local L statistics identifies the areas where a specific air pollutant is considered to be contributing to a type of allergic disease. This study suggests that a bivariate correlation measure between air pollutants and allergic diseases should capture spatially-clustered phenomenon of the association, and detect the local instability in their relationships. It highlights the role of spatial analysis in investigating the contribution of the local-level spatiotemporal dynamics of air pollution to trends and the distribution of allergic diseases.
Credit Evaluation System Based on Blockchain for Multiple Stakeholders in the Food Supply Chain
Authors: Mao Dianhui , Wang Fan , Hao Zhihao , Li Haisheng
Published: August 2018

The food supply chain is a complex system that involves a multitude of “stakeholders” such as farmers, production factories, distributors, retailers and consumers. “Information asymmetry” between stakeholders is one of the major factors that lead to food fraud. Some current researches have shown that applying blockchain can help ensure food safety. However, they tend to study the traceability of food but not its supervision. This paper provides a blockchain-based credit evaluation system to strengthen the effectiveness of supervision and management in the food supply chain. The system gathers credit evaluation text from traders by smart contracts on the blockchain. Then the gathered text is analyzed directly by a deep learning network named Long Short Term Memory (LSTM). Finally traders’ credit results are used as a reference for the supervision and management of regulators. By applying blockchain, traders can be held accountable for their actions in the process of transaction and credit evaluation. Regulators can gather more reliable, authentic and sufficient information about traders. The results of experiments show that adopting LSTM results in better performance than traditional machine learning methods such as Support Vector Machine (SVM) and Navie Bayes (NB) to analyze the credit evaluation text. The system provides a friendly interface for the convenience of users.
Modeling and Predictability of Exchange Rate Changes by the Extended Relative Nelson–Siegel Class of Models
Authors: Ishii Hokuto
Published: August 2018

This paper investigates the predictability of exchange rate changes by extracting the factors from the three-, four-, and five-factor model of the relative Nelson–Siegel class. Our empirical analysis shows that the relative spread factors are important for predicting future exchange rate changes, and our extended model improves the model fitting statistically. The regression model based on the three-factor relative Nelson–Siegel model is the superior model of the extended models for three-month-ahead out-of-sample predictions, and the prediction accuracy is statistically significant from the perspective of the Clark and West statistic. For 6- and 12-month-ahead predictions, although the five-factor model is superior to the other models, the prediction accuracy is not statistically significant.
Satellite-Derived Bathymetry for Improving Canadian Hydrographic Service Charts
Authors: Chénier René , Faucher Marc-André , Ahola Ryan
Published: August 2018

Approximately 1000 Canadian Hydrographic Service (CHS) charts cover Canada’s oceans and navigable waters. Many charts use information collected with techniques that predate the more advanced technologies available to Hydrographic Offices (HOs) today. Furthermore, gaps in survey data, particularly in the Canadian Arctic where only 6% of waters are surveyed to modern standards, are also problematic. Through a Canadian Space Agency (CSA) Government Related Initiatives Program (GRIP) project, CHS is exploring remote sensing techniques to assist with the improvement of Canadian navigational charts. Projects exploring optical/Synthetic Aperture Radar (SAR) shoreline extraction and change detection, as well as optical Satellite-Derived Bathymetry (SDB), are currently underway. This paper focuses on SDB extracted from high-resolution optical imagery, highlighting current results as well as the challenges and opportunities CHS will encounter when implementing SDB within its operational chart production process. SDB is of particular interest to CHS due to its ability to supplement depths derived from traditional hydrographic surveys. This is of great importance in shallow and/or remote Canadian waters where achieving wide-area depth coverage through traditional surveys is costly, time-consuming and a safety risk to survey operators. With an accuracy of around 1 m, SDB could be used by CHS to fill gaps in survey data and to provide valuable information in dynamic areas.
Automation of Building Permission by Integration of BIM and Geospatial Data
Authors: Olsson Per-Ola , Axelsson Josefine , Hooper Martin , Harrie Lars
Published: August 2018

The building permission process is to a large extent an analogue process where much information is handled in paper format or as pdf files. With the ongoing digitalisation in society, there is a potential to automate this process by integrating Building Information Models (BIM) of planned buildings and geospatial data to check if a building conforms to the building permission regulations. In this study, an inventory of which regulations in the (Swedish) detailed development plans that can be automatically checked or supported by 3D visualisation was conducted. Then, two of these regulations, the building height and the building footprint area, were studied in detail to find to which extent they can be automatically checked by integration of BIM and geospatial data. In addition, a feasibility study of one visual criterion was conducted. One concern when automating the building permission process is the variability of content within the Industry Foundation Classes (IFC) data model. Variations in modelling methods and model content leads to differences in IFC models’ content and structure; these differences complicate automated processes. To facilitate automated processes, requirements on the production of IFC models for building permission applications could be defined in the form of model view definitions or delivery specifications.
PEI-Coated Fe3O4 Nanoparticles Enable Efficient Delivery of Therapeutic siRNA Targeting REST into Glioblastoma Cells
Authors: Wang Rui , Degirmenci Volkan , Xin Hongchuan , Li Ying , Wang Liping , Chen Jiayu , Hu Xiaoyu , Zhang Dianbao
Published: August 2018

Glioblastomas (GBM) are the most frequent brain tumors lacking efficient treatment. The increasingly elucidated gene targets make siRNA-based gene therapy a promising anticancer approach, while an efficient delivery system is urgently needed. Here, polyethyleneimine (PEI)-coated Fe3O4 nanoparticles (NPs) have been developed and applied for siRNA delivery into GBM cells to silence repressor element 1-silencing transcription factor (REST). The prepared PEI-coated Fe3O4 NPs were characterized as magnetic nanoparticles with a positive charge, by transmission electronic microscopy, dynamic light-scattering analysis and a magnetometer. By gel retardation assay, the nanoparticles were found to form complexes with siRNA and the interaction proportion of NP to siRNA was 2.8:1. The cellular uptake of NP/siRNA complexes was verified by prussian blue staining, fluorescence labeling and flow cytometry in U-87 and U-251 GBM cells. Furthermore, the REST silencing examined by realtime polymerase chain reaction (PCR) and Western blotting presented significant reduction of REST in transcription and translation levels. Upon the treatment of NP/siRNA targeting REST, the GBM cell viabilities were inhibited and the migration capacities were repressed remarkably, analyzed by cell counting kit-8 and transwell assay separately. In this study, we demonstrated the PEI-coated Fe3O4 nanoparticle as a vehicle for therapeutic siRNA delivery, at an appropriate NP/siRNA weight ratio for REST silencing in GBM cells, inhibiting cell proliferation and migration efficiently. These might represent a novel potential treatment strategy for GBM.
Comparative Proteomic Analysis of Coregulation of CIPK14 and WHIRLY1/3 Mediated Pale Yellowing of Leaves in Arabidopsis
Authors: Guan Zhe , Wang Wanzhen , Yu Xingle , Lin Wenfang , Miao Ying
Published: August 2018

Pale yellowing of leaf variegation is observed in the mutant Arabidopsis lines Calcineurin B-Like-Interacting Protein Kinase14 (CIPK14) overexpression (oeCIPK14) and double-knockout WHIRLY1/WHIRLY3 (why1/3). Further, the relative distribution of WHIRLY1 (WHY1) protein between plastids and the nucleus is affected by the phosphorylation of WHY1 by CIPK14. To elucidate the coregulation of CIPK14 and WHIRLY1/WHIRLY3-mediated pale yellowing of leaves, a differential proteomic analysis was conducted between the oeCIPK14 variegated (oeCIPK14-var) line, why1/3 variegated (why1/3-var) line, and wild type (WT). More than 800 protein spots were resolved on each gel, and 67 differentially abundant proteins (DAPs) were identified by matrix-assisted laser desorption ionization-time of flight/time of flight mass spectrometry (MALDI-TOF/TOF-MS). Of these 67 proteins, 34 DAPs were in the oeCIPK14-var line and 33 DAPs were in the why1/3-var line compared to the WT. Five overlapping proteins were differentially expressed in both the oeCIPK14-var and why1/3-var lines: ATP-dependent Clp protease proteolytic subunit-related protein 3 (ClpR3), Ribulose bisphosphate carboxylase large chain (RBCL), Beta-amylase 3 (BAM3), Ribosome-recycling factor (RRF), and Ribulose bisphosphate carboxylase small chain (RBCS). Bioinformatics analysis showed that most of the DAPs are involved in photosynthesis, defense and antioxidation pathways, protein metabolism, amino acid metabolism, energy metabolism, malate biosynthesis, lipid metabolism, and transcription. Thus, in the why1/3-var and oeCIPK14-var lines, there was a decrease in the photosystem parameters, including the content of chlorophyll, the photochemical efficiency of photosystem (PS II) (Fv/Fm), and electron transport rates (ETRs), but there was an increase in non-photochemical quenching (NPQ). Both mutants showed high sensitivity to intense light. Based on the annotation of the DAPs from both why1/3-var and oeCIPK14-var lines, we conclude that the CIPK14 phosphorylation-mediated WHY1 deficiency in plastids is related to the impairment of protein metabolism, leading to chloroplast dysfunction.
Restraining Akt1 Phosphorylation Attenuates the Repair of Radiation-Induced DNA Double-Strand Breaks and Reduces the Survival of Irradiated Cancer Cells
Authors: Szymonowicz Klaudia , Oeck Sebastian , Krysztofiak Adam , Van der Linden Jansje , Iliakis George , Jendrossek Verena
Published: August 2018

The survival kinase protein kinase B (Akt) participates in the regulation of essential subcellular processes, e.g., proliferation, growth, survival, and apoptosis, and has a documented role in promoting resistance against genotoxic stress including radiotherapy, presumably by influencing the DNA damage response and DNA double-strand break (DSB) repair. However, its exact role in DSB repair requires further elucidation. We used a genetic approach to explore the consequences of impaired phosphorylation of Akt1 at one or both of its key phosphorylation sites, Threonine 308 (T308) or Serine 473 (S473), on DSB repair and radiosensitivity to killing. Therefore, we overexpressed either the respective single or the double phosphorylation-deficient mutants (Akt1-T308A, Akt1-S473A, or Akt1-T308A/S473A) in TRAMPC1 murine prostate cancer cells (TrC1) and measured the DSB repair kinetics and clonogenic cell survival upon irradiation. Only the expression of the Akt1-T308A/S473A induced a significant delay in the kinetics of DSB repair in irradiated TrC1 as determined by the γH2A.X (H2A histone family, member X) assay and the neutral comet assay, respectively. Moreover, Akt1-T308A/S473A-expressing cells were characterized by increased radiosensitivity compared to Akt1-WT (wild type)-expressing cells in long-term colony formation assays. Our data reveal that Akt1’s activation state is important for the cellular radiation response, presumably by modulating the phosphorylation of effector proteins involved in the regulation of DSB repair.
Cardiomyopathies and Related Changes in Contractility of Human Heart Muscle
Authors: Vikhorev Petr G. , Vikhoreva Natalia N.
Published: August 2018

About half of hypertrophic and dilated cardiomyopathies cases have been recognized as genetic diseases with mutations in sarcomeric proteins. The sarcomeric proteins are involved in cardiomyocyte contractility and its regulation, and play a structural role. Mutations in non-sarcomeric proteins may induce changes in cell signaling pathways that modify contractile response of heart muscle. These facts strongly suggest that contractile dysfunction plays a central role in initiation and progression of cardiomyopathies. In fact, abnormalities in contractile mechanics of myofibrils have been discovered. However, it has not been revealed how these mutations increase risk for cardiomyopathy and cause the disease. Much research has been done and still much is being done to understand how the mechanism works. Here, we review the facts of cardiac myofilament contractility in patients with cardiomyopathy and heart failure.
Understanding Ovarian Cancer: iTRAQ-Based Proteomics for Biomarker Discovery
Authors: Swiatly Agata , Horala Agnieszka , Matysiak Jan , Hajduk Joanna , Nowak-Markwitz Ewa , Kokot Zenon J.
Published: August 2018

Despite many years of studies, ovarian cancer remains one of the top ten cancers worldwide. Its high mortality rate is mainly due to lack of sufficient diagnostic methods. For this reason, our research focused on the identification of blood markers whose appearance would precede the clinical manifestation of the disease. ITRAQ-tagging (isobaric Tags for Relative and Absolute Quantification) coupled with mass spectrometry technology was applied. Three groups of samples derived from patients with: ovarian cancer, benign ovarian tumor, and healthy controls, were examined. Mass spectrometry analysis allowed for highlighting the dysregulation of several proteins associated with ovarian cancer. Further validation of the obtained results indicated that five proteins (Serotransferrin, Amyloid A1, Hemopexin, C-reactive protein, Albumin) were differentially expressed in ovarian cancer group. Interestingly, the addition of Albumin, Serotransferrin, and Amyloid A1 to CA125 (cancer antigen 125) and HE4 (human epididymis protein4) improved the diagnostic performance of the model discriminating between benign and malignant tumors. Identified proteins shed light on the molecular signaling pathways that are associated with ovarian cancer development and should be further investigated in future studies. Our findings indicate five proteins with a strong potential to use in a multimarker test for screening and detection of ovarian cancer.
β-Hydroxybutyrate Elicits Favorable Mitochondrial Changes in Skeletal Muscle
Authors: Parker Brian A. , Walton Chase M. , Carr Sheryl T. , Andrus Jacob L. , Cheung Eric C. K. , Duplisea Michael J. , Wilson Esther K. , Draney Carrie , Lathen Daniel R. , Kenner Kyle B. , Thomson David M. , Tessem Jeffery S. , Bikman Benjamin T.
Published: August 2018

The clinical benefit of ketosis has historically and almost exclusively centered on neurological conditions, lending insight into how ketones alter mitochondrial function in neurons. However, there is a gap in our understanding of how ketones influence mitochondria within skeletal muscle cells. The purpose of this study was to elucidate the specific effects of β-hydroxybutyrate (β-HB) on muscle cell mitochondrial physiology. In addition to increased cell viability, murine myotubes displayed beneficial mitochondrial changes evident in reduced H2O2 emission and less mitochondrial fission, which may be a result of a β-HB-induced reduction in ceramides. Furthermore, muscle from rats in sustained ketosis similarly produced less H2O2 despite an increase in mitochondrial respiration and no apparent change in mitochondrial quantity. In sum, these results indicate a general improvement in muscle cell mitochondrial function when β-HB is provided as a fuel.
Ipomoea pes-caprae IpASR Improves Salinity and Drought Tolerance in Transgenic Escherichia coli and Arabidopsis
Authors: Zheng Jie-Xuan , Zhang Hui , Su Hua-Xiang , Xia Kuai-Fei , Jian Shu-Guang , Zhang Mei
Published: August 2018

Ipomoea pes-caprae L. is an extremophile halophyte with strong adaptability to seawater and drought. It is widely used in the ecological restoration of coastal areas or degraded islands in tropical and subtropical regions. In this study, a new abscisic acid, stressandripening (ASR) gene, IpASR, was reported, and is mainly associated with biological functions involved in salt and drought tolerance. Sequence analysis of IpASR showed that this protein contains an ABA/WDS (abscisic acid/water deficit stress) domain, which is a common feature of all plant ASR members. Overexpression of IpASR improved Escherichia coli growth performance compared with the control under abiotic stress treatment. The transgenic overexpressing IpASR Arabidopsis showed higher tolerance to salt and drought stress than the wild type and lower accumulation of hydrogen peroxide (H2O2) and superoxide (O2−) accompanied by increased antioxidant enzyme activity in vivo. IpASR exhibits transcription factor’s activity. Therefore, the overexpression of IpASR in Arabidopsis is supposed to influence the expression of some genes involved in anti-oxidative and abiotic stresses. The results indicate that IpASR is involved in the plant response to salt and drought and probably acts as a reactive oxygen species scavenger or transcription factor, and therefore influences physiological processes associated with various abiotic stresses in plants.
Validation and Evaluation of Reference Genes for Quantitative Real-Time PCR in Macrobrachium Nipponense
Authors: Hu Yuning , Fu Hongtuo , Qiao Hui , Sun Shengming , Zhang Wenyi , Jin Shubo , Jiang Sufei , Gong Yongsheng , Xiong Yiwei , Wu Yan
Published: August 2018

Quantitative real-time PCR (qPCR) is widely used in molecular biology, although the accuracy of the quantitative results is determined by the stability of the reference genes used. Recent studies have investigated suitable reference genes for some crustaceans under various conditions, but studies in Macrobrachium nipponense are currently lacking. In this study, we selected the following seven genes from among 35 commonly used housekeeping genes as candidate qPCR reference genes for temporal and spatial expression: EIF (eukaryotic translation initiation factor 5A), 18S (18S ribosomal RNA), EF-1α (elongation factor-1α), GAPDH (glyceraldehyde-3-phosphate dehydrogenase), TUB (α-tubulin), β-act (β-actin), and RPL18 (Ribosomal protein L18). The stability of each reference gene was evaluated by GeNorm, NormFinder, BestKeeper, and comparative ∆C t methods, and was comprehensively ranked using RefFinder. RPL18 was shown to be the most suitable reference gene for adult M. nipponense tissues, while EIF was the most stable in different ovarian and embryo stages and in white spot syndrome virus infection, and β-act was the most stable reference gene under hypoxia stress. The reliability of the rankings was confirmed by RNA interference experiments. To the best of our knowledge, this represents the first systematic analysis of reference genes for qPCR experiments in M. nipponense, and the results will provide invaluable information for future research in closely related crustaceans.
Heme Oxygenase 1 in the Nervous System: Does It Favor Neuronal Cell Survival or Induce Neurodegeneration?
Authors: Nitti Mariapaola , Piras Sabrina , Brondolo Lorenzo , Marinari Umberto Maria , Pronzato Maria Adelaide , Furfaro Anna Lisa
Published: August 2018

Heme oxygenase 1 (HO-1) up-regulation is recognized as a pivotal mechanism of cell adaptation to stress. Under control of different transcription factors but with a prominent role played by Nrf2, HO-1 induction is crucial also in nervous system response to damage. However, several lines of evidence have highlighted that HO-1 expression is associated to neuronal damage and neurodegeneration especially in Alzheimer’s and Parkinson’s diseases. In this review, we summarize the current literature regarding the role of HO-1 in nervous system pointing out different molecular mechanisms possibly responsible for HO-1 up-regulation in nervous system homeostasis and neurodegeneration.
Operations and Aggregation Methods of Single-Valued Linguistic Neutrosophic Interval Linguistic Numbers and Their Decision Making Method
Authors: Ye Jun , Cui Wenhua
Published: August 2018

To comprehensively describe uncertain/interval linguistic arguments and confident linguistic arguments in the decision making process by a linguistic form, this study first presents the concept of a single-valued linguistic neutrosophic interval linguistic number (SVLN-ILN), which is comprehensively composed of its uncertain/interval linguistic number (determinate linguistic argument part) and its single-valued linguistic neutrosophic number (confident linguistic argument part), and its basic operations. Then, the score function of SVLN-ILN based on the attitude index and confident degree/level is presented for ranking SVLN-ILNs. After that, SVLN-ILN weighted arithmetic averaging (SVLN-ILNWAA) and SVLN-ILN weighted geometric averaging (SVLN-ILNWGA) operators are proposed to aggregate SVLN-ILN information and their properties are investigated. Further, a multi-attribute decision-making (MADM) method based on the proposed SVLN-ILNWAA or SVLN-ILNWGA operator and the score function is established under consideration of decision makers’ preference attitudes (pessimist, moderate, and optimist). Lastly, an actual example is given to show the applicability of the established MADM approach with decision makers’ attitudes.
Mechanical Milling-Assisted Spark Plasma Sintering of Fine-Grained W-Ni-Mn Alloy
Authors: Pan Yanlin , Xiang Daoping , Wang Ning , Li Hui , Fan Zhishuai
Published: August 2018

Fine-grained W-6Ni-4Mn alloys were fabricated by spark plasma sintering (SPS) using mechanical milling W, Ni and Mn composite powders. The relative density of W-6Ni-4Mn alloy increases from 71.56% to 99.60% when it is sintered at a low temperature range of 1000–1200 °C for 3 min. The spark plasma sintering process of the alloy can be divided into three stages, which clarify the densification process of powder compacts. As the sintering temperature increases, the average W grain size increases but remains at less than 7 µm and the distribution of the binding phase is uniform. Transmission electron microscopy (TEM) observation reveals that the W-6Ni-4Mn alloy consists of the tungsten phase and the γ-(Ni, Mn, W) binding phase. As the sintering temperature increases, the Rockwell hardness and bending strength of alloys initially increases and then decreases. The optimum comprehensive hardness and bending strength of the alloy are obtained at 1150 °C. The main fracture mode of the alloys is W/W interface fracture.
Investigation of Ageing in Bitumen Using Fluorescence Spectrum
Authors: Tang Ning , Yang Yu-Li , Yu Mei-Ling , Wang Wen-Li , Cao Shi-Yue , Wang Qing , Pan Wen-Hao
Published: August 2018

Bitumen ageing is a very complex process and poses a threat to the performance of pavements. In the present work, a fluorescence spectrophotometer was employed to research the change rule of components and the structure of bitumen after the ageing process. The Thin Film Oven Test (TFOT) and Ultraviolet (UV) light treatment were carried out as ageing methods. The properties and components of bitumen were tested before and after aging. The 2D and 3D fluorescence spectra of bitumen were analyzed. The vector of fluorescence peak was calculated for evaluating the ageing process. The results indicated that the ideal concentration of bitumen- tetrachloromethane solution was 0.1 g/L or smaller for avoiding the fluorescence quenching. The coordinates of fluorescent peak appeared “blue-shift” after ageing due to the change of aromatics. In addition, bitumen has already occurred serious ageing when the magnitude of a vector is more than 36.
Influence of the Preparation Method and Photo-Oxidation Treatment on the Thermal and Gas Transport Properties of Dense Films Based on a Poly(ether-block-amide) Copolymer
Authors: Clarizia Gabriele , Bernardo Paola , Gorrasi Giuliana , Zampino Daniela , Carroccio Sabrina C.
Published: August 2018

Dense films based on the hydrophobic Pebax®2533 were prepared by using solution casting in different solvents as well as compression molding and subjected to photo–aging under ultraviolet (UV) irradiation. The influence of the preparation method, including the casting solvents, as well as the UV irradiation time selected to treat the samples, were evaluated in terms of permeation rates of pure gases (CO2, N2, O2, CH4, He, and H2). The transport data were correlated with the microstructure and surface properties by using differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS), as well as water contact angle measurements. The obtained results showed that a controlled photo-oxidation process reduces the hydrophobicity of the Pebax®2533 films, increasing their permeability without compromising their integrity.
Effects of Processing Conditions on Texture and Microstructure Evolution in Extra-Low Carbon Steel during Multi-Pass Asymmetric Rolling
Authors: Dhinwal Satyaveer Singh , Toth Laszlo S. , Hodgson Peter Damian , Haldar Arunansu
Published: August 2018

Multi-pass rolling was carried out on extra-low carbon steel at room temperature by imposing different ratios of asymmetry in the roll-diameters as well as by conventional mode. The aim of this study is to understand the effect of shear deformation due to the asymmetric conditions on the development of the rolling texture and the possibilities of propagating the shear deformation into the mid-thickness area of the sheet. The trends of the measured texture developments in both symmetric and asymmetric rolling indicate their dependence primarily on the stability and fraction of the Goss {110}<001> and the rotated cube {001}<111> orientations. The effects of asymmetry conditions were further examined on the microstructure evolution and were correlated to the increased orientation inhomogeneity and grain fragmentation. Both texture and microstructure development showed their dependence on the applied thickness reduction per pass, on the total thickness reduction of the sheet as well as on the degree of the imposed asymmetry. It was found that shear textures can be obtained by asymmetric rolling at conditions where all three parameters—asymmetry ratio, strain in one pass, and the total accumulated strain—are as large as possible.
Accelerating High-Throughput Screening for Structural Materials with Production Management Methods
Authors: Bader Alexander , Meiners Finn , Tracht Kirsten
Published: August 2018

High-throughput screenings are widely accepted for pharmaceutical developments for new substances and the development of new drugs with required characteristics by evolutionary studies. Current research projects transfer this principle of high-throughput testing to the development of metallic materials. In addition to new generating and testing methods, these types of high-throughput systems need a logistical control and handling method to reduce throughput time to get test results faster. Instead of the direct material flow found in classical high-throughput screenings, these systems have a very complex structure of material flow. The result is a highly dynamic system that includes short-term changes such as rerun stations, partial tests, and temporarily paced sequences between working systems. This paper presents a framework that divides the actions for system acceleration into three main sections. First, methods for special applications in high-throughput systems are designed or adapted to speed up the generation, treatment, and testing processes. Second, methods are needed to process trial plans and to control test orders, which can efficiently reduce waiting times. The third part of the framework describes procedures for handling samples. This reduces non-productive times and reduces order processing in individual lots.
Approximate Dynamic Programming Based Control of Proppant Concentration in Hydraulic Fracturing
Authors: Singh Sidhu Harwinder , Siddhamshetty Prashanth , Kwon Joseph S.
Published: August 2018

Hydraulic fracturing has played a crucial role in enhancing the extraction of oil and gas from deep underground sources. The two main objectives of hydraulic fracturing are to produce fractures with a desired fracture geometry and to achieve the target proppant concentration inside the fracture. Recently, some efforts have been made to accomplish these objectives by the model predictive control (MPC) theory based on the assumption that the rock mechanical properties such as the Young’s modulus are known and spatially homogenous. However, this approach may not be optimal if there is an uncertainty in the rock mechanical properties. Furthermore, the computational requirements associated with the MPC approach to calculate the control moves at each sampling time can be significantly high when the underlying process dynamics is described by a nonlinear large-scale system. To address these issues, the current work proposes an approximate dynamic programming (ADP) based approach for the closed-loop control of hydraulic fracturing to achieve the target proppant concentration at the end of pumping. ADP is a model-based control technique which combines a high-fidelity simulation and function approximator to alleviate the “curse-of-dimensionality” associated with the traditional dynamic programming (DP) approach. A series of simulations results is provided to demonstrate the performance of the ADP-based controller in achieving the target proppant concentration at the end of pumping at a fraction of the computational cost required by MPC while handling the uncertainty in the Young’s modulus of the rock formation.
Biochemical Characterization and Degradation Pattern of a Novel Endo-Type Bifunctional Alginate Lyase AlyA from Marine Bacterium Isoptericola halotolerans
Authors: Zhu Benwei , Ning Limin , Jiang Yucui , Ge Lin
Published: August 2018

Alginate lyases are important tools to prepare oligosaccharides with various physiological activities by degrading alginate. Particularly, the bifunctional alginate lyase can efficiently hydrolyze the polysaccharide into oligosaccharides. Herein, we cloned and identified a novel bifunctional alginate lyase, AlyA, with a high activity and broad substrate specificity from bacterium Isoptericola halotolerans NJ-05 for oligosaccharides preparation. For further applications in industry, the enzyme has been characterized and its action mode has been also elucidated. It exhibited the highest activity (7984.82 U/mg) at pH 7.5 and 55 °C. Additionally, it possessed a broad substrate specificity, showing high activities towards not only polyM (polyβ-d-mannuronate) (7658.63 U/mg), but also polyG (poly α-l-guluronate) (8643.29 U/mg). Furthermore, the Km value of AlyA towards polyG (3.2 mM) was lower than that towards sodium alginate (5.6 mM) and polyM (6.7 mM). TLC (Thin Layer Chromatography) and ESI-MS (Electrospray Ionization Mass Spectrometry) were used to study the action mode of the enzyme, showing that it can hydrolyze the substrates in an endolytic manner to release a series of oligosaccharides such as disaccharide, trisaccharide, and tetrasaccharide. This study provided extended insights into the substrate recognition and degrading pattern of the alginate lyases, with a broad substrate specificity.
Marine Compounds and Autophagy: Beginning of a New Era
Authors: Dyshlovoy Sergey A. , Honecker Friedemann
Published: August 2018

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Antitumor Activity of Quinazolinone Alkaloids Inspired by Marine Natural Products
Authors: Long Solida , Resende Diana I. S. P. , Kijjoa Anake , Silva Artur M. S. , Pina André , Fernández-Marcelo Tamara , Vasconcelos M. Helena , Sousa Emília , Pinto Madalena M. M.
Published: August 2018

Many fungal quinazolinone metabolites, which contain the methyl-indole pyrazino [1,2-b]quinazoline-3,6-dione core, have been found to possess promising antitumor activity. The purpose of this work was to synthesize the enantiomeric pairs of two members of this quinazolinone family, to explore their potential as antitumor and their ability to revert multidrug resistance. The marine natural product fiscalin B (4c), and antienantiomers (4b, 5b, and 5c) were synthesized via a one-pot approach, while the syn enantiomers (4a, 4d, 5a, and 5d) were synthetized by a multi-step procedure. These strategies used anthranilic acid (i), chiral N-protected α-amino acids (ii), and tryptophan methyl esters (iii) to form the core ring of pyrazino[2,1-b]quinazoline-3,6-dione scaffold. Four enantiomeric pairs, with different enantiomeric purities, were obtained with overall yields ranging from 7 to 40%. Compounds 4a–d and 5a–d were evaluated for their growth inhibitory effect against two tumor cell lines. Differences between enantiomeric pairs were noted and 5a–d displayed GI50 values ranging from 31 to 52 μM, which are lower than those of 4a–d. Nevertheless, no effect on P-glycoprotein (P-gp) modulation was observed for all compounds. This study disclosed new data for fiscalin B (4c), as well as for its analogues for a future development of novel anticancer drug leads.
A Novel Peptide, Nicotinyl–Isoleucine–Valine–Histidine (NA–IVH), Promotes Antioxidant Gene Expression and Wound Healing in HaCaT Cells
Authors: Son Dong Hwee , Yang Dong Joo , Sun Ji Su , Kim Seul Ki , Kang Namju , Kang Jung Yun , Choi Yun-Hee , Lee Jeong Hun , Moh Sang Hyun , Shin Dong Min , Kim Ki Woo
Published: August 2018

Nicotinamide (NA), a water-soluble vitamin B3, has been shown to exert cellular-protective effects against reactive oxygen species (ROS). In order to improve the cellular-protective effects of NA, we synthesized a novel compound, nicotinyl–isoleucine–valine–histidine (NA–IVH), by combining NA with jellyfish peptides’ IVH. In the present study, we examined the cellular-protective effects of the novel synthetic nicotinyl-peptide, NA–IVH. We found that NA–IVH enhances the radical scavenging activity with a robust increase of the nuclear factor (erythroid-derived 2)-like factor (Nrf2) expression in human HaCaT keratinocytes. In addition, NA–IVH protected the cells from hydrogen peroxide (H2O2)-induced cell death. Interestingly, NA–IVH exhibited an improved wound-healing effect in a high glucose condition, possibly through the regulation of reactive oxygen species (ROS). Collectively, our results imply that a novel nicotinyl-peptide, NA–IVH, has a wound-healing effect in a hyperglycemic condition, possibly by modulating excessive ROS.
The Protective Effects of Clams on Hypercholesterolemia in Late-Stage Triple-Transgenic Alzheimer’s Diseased Mice Hearts
Authors: Hsieh You-Liang , Teng Hsu-Ju , Yeh Yen-Hung , Hsieh Cheng-Hong , Huang Chih-Yang
Published: August 2018

To investigate a high cholesterol diet in Alzheimer’s disease (AD) mice, they were fed with (2% cholesterol) in five groups with a control group, AD mice group, AD mice plus Meretrix lusoria group, AD mice plus Geloina eros group, and, AD mice plus Corbicula fluminea group for three months, and treated with the fatty acid profiles of clams by gas chromatography (GC). The results showed that treatment with clams for three months reduced Fas/L and Caspase-3 in the Meretrix lusoria and Geloina eros groups, but Fas-associated death domain (FADD) and Caspase-8 were strongly reduced in the Geloina eros group. For the mitochondria-dependent apoptotic pathway, the reduction of apoptosis proteins were observed in the hearts of clams-treated AD mice. BAK and Caspase-9 was reduced in the Meretrix lusoria group, but Caspase-3 and Cytochrome-c were reduced in Geloina eros group. Enhancement of survival proteins p-AKT, p-IGF1R, p-PI3K, Bcl-XL, Bcl2, and the longevity SIRT1 signaling proteins, p-AMPK-α, SIRT1, PGC1-α, p-FOXO3 were observed in clams-treated mice and even more strongly enhanced in the Meretrix lusoria, Geloina eros and Corbicula fluminea groups. This study observed that the ingestion of clams caused a reduction of apoptosis proteins and enhancement of survival and SIRT1 signaling proteins in the hearts.