bluuebunny/crossref_metadata_2025 huggingface dataset:
Modeling of Tractor Fuel Consumption
Authors: Kolator Bronisław Andrzej
Published: April 2021

In this paper, the energy diagnostic of tractor performance consists in evaluating the energy (fuel consumption per hectare—dm3 ha−1) for a given agricultural operation and in combining it with working capacity, also called productivity (area productivity—ha h−1). One of the methods of solving this problem is the identification of the functioning process of the machine unit. A model of the process of the machine unit performance was developed, considering the operation of the rear linkage system of the implement with the force control adjustment system. In order to analyze the system, a mathematical model of the system function was built: tractor-implement-soil, defining the physical connections and interdependencies between the individual subsystems of the system. Based on this model, a simulation model was developed and implemented in the Matlab/Simulink environment. The Simulink package was used to test the performance of the machine set. The efficiency indicators according to the adopted criteria were calculated in the evaluation block. To evaluate the process, the technical and operational parameters of the tractor, the type and parameters of the tool, and soil properties were taken into account. The results of simulation studies obtained on a validated model are consistent with experimental data from appropriate soil conditions.
Environmental Life-Cycle Assessment of an Innovative Multifunctional Toilet
Authors: Rodrigues Carla , Almeida João , Santos Maria Inês , Costa Andreia , Além Sandra , Rufo Emanuel , Tadeu António , Freire Fausto
Published: April 2021

Innovative toilets can save resources, but have higher embodied impacts associated with materials and electronic components. This article presents an environmental life-cycle assessment (LCA) of an innovative multifunctional toilet (WashOne) for two alternative configurations (with or without washlet), comparing its performance with those of conventional systems (toilet and bidet). Additionally, two scenario analyses were conducted: (i) user behavior (alternative washlet use patterns) and (ii) user location (Portugal, Germany, the Netherlands, Sweden and Saudi Arabia). The results show that the WashOne with washlet has a better global environmental performance than the conventional system, even for low use. It also reveals that the use phase has the highest contribution to impacts due to electricity consumption. User location analysis further shows that Sweden has the lowest environmental impact, while Germany and the Netherlands have the highest potential for impact reduction when changing from a conventional system to the WashOne. Based on the overall results, some recommendations are provided to enhance the environmental performance of innovative toilet systems, namely the optimization of the washlet use patterns. This article highlights the importance of performing a LCA at an early stage of the development of innovative toilets by identifying the critical issues and hotspots to improve their design and performance.
Influence of Slope Direction on the Soil Seed Bank and Seedling Regeneration of Castanopsis hystrix Seed Rain
Authors: Zhao Zong , Liu Yong , Jia Hongyan , Sun Wensheng , Ming Angang , Pang Shengjiang , An Ning , Zhang Jihui , Tang Chuang , Dong Shitao
Published: April 2021

Objective: To investigate the impact of different slope directions on the quantity and quality of the soil seed bank and seedling germination process of Castanopsis hystrix plantations. Method: Fixed sample plots in forest stands of Castanopsis hystrix were established on different slope directions (sunny slope, semi-sunny slope, semi-shady slope, and shady slope). The characteristics of the forest stand were investigated, and per-wood scaling was carried out. The temporal dynamics of the seed rain and seed bank were quantified using seed rain collectors and by collecting soil samples from different depths. The quantity and quality of the seeds were determined, and the vigor of mature seeds was measured throughout the study. Results: (1) The diffusion of Castanopsis hystrix seed rain started in mid-September, reached its peak from late October to early November, and ended in mid-December. (2) The dissemination process, occurrence time, and composition of the seed rain varied between the different slope directions. The seed rain intensity on the semi-sunny slope was the highest (572.75 ± 9.50 grains∙m−2), followed by the sunny slope (515.60 ± 10.28 grains∙m−2), the semi-shady slope (382.13 ± 12.11 grains∙m−2), and finally the shady slope (208.00 ± 11.35 grains∙m−2). The seed rain on the sunny slope diffused earliest and lasted the longest, while the seed rain on the shady slope diffused latest and lasted the shortest time. Seed vigor and the proportion of mature seeds within the seed rain were greatest on the semi-sunny slope, followed by the sunny slope, semi-shady slope, and the shady slope. (3) From the end of the seed rain to August of the following year, the amount of total reserves of the soil seed banks was highest on the semi-sunny slope, followed by the sunny slope then the semi-shady slope, and it was the lowest on the shady slope. The amount of mature, immature, gnawed seeds and seed vigor of the soil seed bank in various slope directions showed a decreasing trend with time. The seeds of the seed bank in all slope directions were mainly distributed in the litter layer, followed by the 0–2 cm humus layer, and only a few seeds were present in the 2–5 cm soil layer. (4) The seedling density of Castanopsis hystrix differed significantly on the different slope directions. The semi-sunny slope had the most seedlings, followed by the sunny slope, semi-shady slope, and the shady slope. Conclusions: The environmental conditions of the semi-sunny slope were found to be most suitable for the seed germination and seedling growth of Castanopsis hystrix, and more conducive to the regeneration and restoration of its population.
Optimization Model of Phenolics Encapsulation Conditions for Biofortification in Fatty Acids of Animal Food Products
Authors: Tolve Roberta , Galgano Fernanda , Condelli Nicola , Cela Nazarena , Lucini Luigi , Caruso Marisa Carmela
Published: April 2021

The nutritional quality of animal products is strongly related to their fatty acid content and composition. Nowadays, attention is paid to the possibility of producing healthier foods of animal origin by intervening in animal feed. In this field, the use of condensed tannins as dietary supplements in animal nutrition is becoming popular due to their wide range of biological effects related, among others, to their ability to modulate the rumen biohydrogenation and biofortify, through the improvement of the fatty acids profile, the derivate food products. Unfortunately, tannins are characterized by strong astringency and low bioavailability. These disadvantages could be overcome through the microencapsulation in protective matrices. With this in mind, the optimal conditions for microencapsulation of a polyphenolic extract rich in condensed tannins by spray drying using a blend of maltodextrin (MD) and gum Arabic (GA) as shell material were investigated. For this purpose, after the extract characterization, through spectrophotometer assays and ultra-high-performance liquid chromatography-quadrupole time-of-flight (UHPLC-QTOF) mass spectrometry, a central composite design (CCD) was employed to investigate the combined effects of core:shell and MD:GA ratio on the microencapsulation process. The results obtained were used to develop second-order polynomial regression models on different responses, namely encapsulation yield, encapsulation efficiency, loading capacity, and tannin content. The formulation characterized by a core:shell ratio of 1.5:5 and MD:GA ratio of 4:6 was selected as the optimized one with a loading capacity of 17.67%, encapsulation efficiency of 76.58%, encapsulation yield of 35.69%, and tannin concentration of 14.46 g/100 g. Moreover, in vitro release under varying pH of the optimized formulation was carried out with results that could improve the use of microencapsulated condensed tannins in animal nutrition for the biofortification of derivates.
Study on the Sleep-Improvement Effects of Hemerocallis citrina Baroni in Drosophila melanogaster and Targeted Screening to Identify Its Active Components and Mechanism
Authors: Liang Yuxuan , Huang Riming , Chen Yongchun , Zhong Jing , Deng Jie , Wang Ziyi , Wu Zhuojun , Li Meiying , Wang Hong , Sun Yuanming
Published: April 2021

Hemerocallis citrina Baroni (HC) is an edible plant in Asia, and it has been traditionally used for sleep-improvement. However, the bioactive components and mechanism of HC in sleep-improvement are still unclear. In this study, the sleep-improvement effect of HC hydroalcoholic extract was investigated based on a caffeine-induced insomnia model in Drosophila melanogaster (D. melanogaster), and the ultrahigh-performance liquid chromatography coupled with electrospray ionization quadrupole Orbitrap high-resolution mass spectrometry (UHPLC-ESI-Orbitrap-MS) and network pharmacology strategy were further combined to screen systematically the active constituents and mechanism of HC in sleep-improvement. The results suggested HC effectively regulated the number of nighttime activities and total sleep time of D. melanogaster in a dose-dependent manner and positively regulated the sleep bouts and sleep duration of D. melanogaster. The target screening suggested that quercetin, luteolin, kaempferol, caffeic acid, and nicotinic acid were the main bioactive components of HC in sleep-improvements. Moreover, the core targets (Akt1, Cat, Ple, and Sod) affected by HC were verified by the expression of the mRNA of D. melanogaster. In summary, this study showed that HC could effectively regulate the sleep of D. melanogaster and further clarifies the multi-component and multi-target features of HC in sleep-improvement, which provides a new insight for the research and utilization of HC.
Pseudomonas spp.: Are Food Grade Organic Acids Efficient against These Spoilage Microorganisms in Fresh Cheeses?
Authors: Tirloni Erica , Bernardi Cristian , Stella Simone
Published: April 2021

Psychrotolerant Pseudomonas spp. are among the most common spoilage agents in fresh, soft and semi-soft cheeses; therefore, hurdles inhibiting their growth are in strong demand by producers. This study aimed to establish Minimal Inhibiting Concentrations (MICs) of lactic and acetic acid towards P. fluorescens and to evaluate the efficacy of a cheese surface treatment with these two organic acids. MICs were determined in Brain Heart Infusion broth at 30 °C: the inhibition was achieved at a concentration of 49.96 mM and 44.40 mM of acetic and lactic acid, respectively. Two series of inhibition tests were performed on fresh “Primo sale” cheese, inoculated with P. brenneri MGM3, then dipped into different acid solutions (acetic acid: 49.96, 99.92 and 149.88 mM; lactic acid: 44.40, 88.80 and 133.20 mM) and stored at 6 °C. P. brenneri MGM3 were enumerated, including a control series. A significantly lower growth was revealed at the highest concentrations tested, both for acetic (p < 0.01) and lactic acid (p < 0.05) if compared to control samples. A conditioning of “Primo sale” surface with organic acid solutions could be a useful hurdle for Pseudomonas inhibition and shelf-life extension; it should be applied in combination with other mild interventions to fight spoilage and maintain the original product characteristics.
FODMAP Fingerprinting of Bakery Products and Sourdoughs: Quantitative Assessment and Content Reduction through Fermentation
Authors: Pitsch Johannes , Sandner Georg , Huemer Jakob , Huemer Maximilian , Huemer Stefan , Weghuber Julian
Published: April 2021

Fermentable oligo-, di-, and monosaccharides and polyols (FODMAPs) are associated with digestive disorders and with diseases such as irritable bowel syndrome. In this study, we determined the FODMAP contents of bread, bakery products, and flour and assessed the effectiveness of sourdough fermentation for FODMAP reduction. The fermentation products were analyzed to determine the DP 2–7 and DP >7 fructooligosaccharide (FOS) content of rye and wheat sourdoughs. FOSs were reduced by Acetobacter cerevisiae, Acetobacter okinawensis, Fructilactobacillus sanfranciscensis, and Leuconostoc citreum to levels below those in rye (−81%; −97%) and wheat (−90%; −76%) flours. The fermentation temperature influenced the sourdough acetic acid to lactic acid ratios (4:1 at 4 °C; 1:1 at 10 °C). The rye sourdough contained high levels of beneficial arabinose (28.92 g/kg) and mannitol (20.82 g/kg). Our study contributes in-depth knowledge of low-temperature sourdough fermentation in terms of effective FODMAP reduction and concurrent production of desirable fermentation byproducts.
Identification of Novel Loci and Candidate Genes for Resistance to Powdery Mildew in a Resequenced Cucumber Germplasm
Authors: Liu Xiaoping , Gu Xingfang , Lu Hongwei , Liu Panna , Miao Han , Bai Yuling , Zhang Shengping
Published: April 2021

Powdery mildew (PM) is one of the most serious diseases in cucumber and causes huge yield loss. Multiple quantitative trait loci (QTLs) for PM resistance have been reported in previous studies using a limited number of cucumber accessions. In this study, a cucumber core germplasm (CG) consisting of 94 resequenced lines was evaluated for PM resistance in four trials across three years (2013, 2014, and 2016). These trials were performed on adult plants in the field with natural infection. Using genome-wide association study (GWAS), 13 loci (pmG1.1, pmG1.2, pmG2.1, pmG2.2, pmG3.1, pmG4.1, pmG4.2, pmG5.1, pmG5.2, pmG5.3, pmG5.4, pmG6.1, and pmG6.2) associated with PM resistance were detected on all chromosomes except for Chr.7. Among these loci, ten were mapped to chromosomal intervals where QTLs had been reported in previous studies, while, three (pmG2.1, pmG3.1, and pmG4.1) were novel. The loci of pmG2.1, pmG5.2, pmG5.3 showed stronger signal in four trials. Based on the annotation of homologous genes in Arabidopsis and pairwise LD correlation analysis, candidate genes located in the QTL intervals were predicted. SNPs in these candidate genes were analyzed between haplotypes of highly resistant (HR) and susceptible (HS) CG lines, which were defined based on combing disease index data of all trials. Furthermore, candidate genes (Csa5G622830 and CsGy5G015660) reported in previous studies for PM resistance and cucumber orthologues of several PM susceptibility (S) genes (PMR5, PMR-6, and MLO) that are colocalized with certain QTLs, were analyzed for their potential contribution to the QTL effect on both PM and DM in the CG population. This study shows that the CG germplasm is a very valuable resource carrying known and novel QTLs for both PM and DM resistance, which can be exploited in cucumber breeding.
LncMyoD Promotes Skeletal Myogenesis and Regulates Skeletal Muscle Fiber-Type Composition by Sponging miR-370-3p
Authors: Zhang Peiwen , Du Jingjing , Guo Xinyu , Wu Shuang , He Jin , Li Xinrong , Shen Linyuan , Chen Lei , Li Bohong , Zhang Jingjun , Xie Yuhao , Niu Lili , Jiang Dongmei , Li Xuewei , Zhang Shunhua , Zhu Li
Published: April 2021

The development of skeletal muscle is a highly ordered and complex biological process. Increasing evidence has shown that noncoding RNAs, especially long-noncoding RNAs (lncRNAs) and microRNAs, play a vital role in the development of myogenic processes. In this study, we observed that lncMyoD regulates myogenesis and changes myofiber-type composition. miR-370-3p, which is directly targeted by lncMyoD, promoted myoblast proliferation and inhibited myoblast differentiation in the C2C12 cell line, which serves as a valuable model for studying muscle development. In addition, the inhibition of miR-370-3p promoted fast-twitch fiber transition. Further analysis indicated that acyl-Coenzyme A dehydrogenase, short/branched chain (ACADSB) is a target gene of miR-370-3p, which is also involved in myoblast differentiation and fiber-type transition. Furthermore, our data suggested that miR-370-3p was sponged by lncMyoD. In contrast with miR-370-3p, lncMyoD promoted fast-twitch fiber transition. Taken together, our results suggest that miR-370-3p regulates myoblast differentiation and muscle fiber transition and is sponged by lncMyoD.
Neuromesodermal Lineage Contribution to CNS Development in Invertebrate and Vertebrate Chordates
Authors: Hudson Clare , Yasuo Hitoyoshi
Published: April 2021

Ascidians are invertebrate chordates and the closest living relative to vertebrates. In ascidian embryos a large part of the central nervous system arises from cells associated with mesoderm rather than ectoderm lineages. This seems at odds with the traditional view of vertebrate nervous system development which was thought to be induced from ectoderm cells, initially with anterior character and later transformed by posteriorizing signals, to generate the entire anterior-posterior axis of the central nervous system. Recent advances in vertebrate developmental biology, however, show that much of the posterior central nervous system, or spinal cord, in fact arises from cells that share a common origin with mesoderm. This indicates a conserved role for bi-potential neuromesoderm precursors in chordate CNS formation. However, the boundary between neural tissue arising from these distinct neural lineages does not appear to be fixed, which leads to the notion that anterior-posterior patterning and neural fate formation can evolve independently.
Human Mitochondrial Control Region and mtGenome: Design and Forensic Validation of NGS Multiplexes, Sequencing and Analytical Software
Authors: Holt Cydne L. , Stephens Kathryn M. , Walichiewicz Paulina , Fleming Keenan D. , Forouzmand Elmira , Wu Shan-Fu
Published: April 2021

Forensic mitochondrial DNA (mtDNA) analysis conducted using next-generation sequencing (NGS), also known as massively parallel sequencing (MPS), as compared to Sanger-type sequencing brings modern advantages, such as deep coverage per base (herein referred to as read depth per base pair (bp)), simultaneous sequencing of multiple samples (libraries) and increased operational efficiencies. This report describes the design and developmental validation, according to forensic quality assurance standards, of end-to-end workflows for two multiplexes, comprised of ForenSeq mtDNA control region and mtDNA whole-genome kits the MiSeq FGxTM instrument and ForenSeq universal analysis software (UAS) 2.0/2.1. Polymerase chain reaction (PCR) enrichment and a tiled amplicon approach target small, overlapping amplicons (60–150 bp and 60–209 bp for the control region and mtGenome, respectively). The system provides convenient access to data files that can be used outside of the UAS if desired. Studies assessed a range of environmental and situational variables, including but not limited to buccal samples, rootless hairs, dental and skeletal remains, concordance of control region typing between the two multiplexes and as compared to orthogonal data, assorted sensitivity studies, two-person DNA mixtures and PCR-based performance testing. Limitations of the system and implementation considerations are discussed. Data indicated that the two mtDNA multiplexes, MiSeq FGx and ForenSeq software, meet or exceed forensic DNA quality assurance (QA) guidelines with robust, reproducible performance on samples of various quantities and qualities.
An Analytical Solution for Pressure-Induced Deformation of Anisotropic Multilayered Subsurface
Authors: Park Joonsang , Bjørnarå Tore Ingvald , Bohloli Bahman
Published: April 2021

We present a generalized Geertsma solution that can consider any number of finite-thickness layers in the subsurface whose mechanical properties are different from layer to layer. In addition, each layer can be assumed either isotropic or anisotropic. The accuracy of the generalized solution is validated against a numerical reference solution. The generalized Geertsma solution is further extended by a linear superposition framework that enables a response simulation due to an arbitrarily-distributed non-uniform pressure anomaly. The linear superposition approach is tested and validated by solving a realistic synthetic model based on the In Salah CO2 storage model and compared with a full 3D finite element solution. Finally, by means of a simple inversion exercise (based on the linear superposition approach), we learn that the stiffnesses of cap rock and reservoir are the most influencing parameter on the inversion result for a given layering geometry, suggesting that it is very important to estimate high-confidence mechanical properties of both cap rock and reservoir.
Psychopathology and Somatic Complaints: A Cross-Sectional Study with Portuguese Adults
Authors: Becker Joana Proença , Paixão Rui , Quartilho Manuel João
Published: April 2021

(1) Background: Functional somatic symptoms (FSS) are physical symptoms that cannot be fully explained by medical diagnosis, injuries, and medication intake. More than the presence of unexplained symptoms, this condition is associated with functional disabilities, psychological distress, increased use of health services, and it has been linked to depressive and anxiety disorders. Recognizing the difficulty of diagnosing individuals with FSS and the impact on public health systems, this study aimed to verify the concomitant incidence of psychopathological symptoms and FSS in Portugal. (2) Methods: For this purpose, 93 psychosomatic outpatients (91.4% women with a mean age of 53.9 years old) and 101 subjects from the general population (74.3% women with 37.8 years old) were evaluated. The survey questionnaire included the 15-item Patient Health Questionnaire, the 20-Item Short Form Survey, the Brief Symptom Inventory, the Depression, Anxiety and Stress Scale, and questions on sociodemographic and clinical characteristics. (3) Results: Increases in FSS severity were correlated with higher rates of depression, anxiety, and stress symptoms. The findings also suggest that increased rates of FSS are associated with lower educational level and female gender. (4) Conclusion: Being aware of the relationship between FSS and psychopathological symptoms and the need to explore psychosocial issues during clinical interviews may favor early detection of these cases. The early detection of mental disorders is essential for individuals’ adherence to treatments, reflecting on healthcare costs.
Peach, Apple, and Pear Fruit Quality: To Peel or Not to Peel?
Authors: Michailidis Michail , Karagiannis Evangelos , Nasiopoulou Elpida , Skodra Christina , Molassiotis Athanassios , Tanou Georgia
Published: April 2021

The nutritional value of the peeled and unpeeled fruit (peel plus flesh tissues) was studied using four peach (Prunus persica L.; Red Heaven, Maria Blanca, Big Top, and Queen Giant), two pear (Pyrus communis L.; Santa Maria, Pyrus pyrifolia N.; Nashi), and three apple (Malus domestica Borkh.; Gala, Granny Smith, and Red Chief) cultivars. Based on principal components analysis (PCA) models, there was a clear differentiation among the cultivars’ and the peeled fruits’ nutritional characteristics in comparison to the unpeeled ones. Increased antioxidant capacity and content of total phenols and flavonoids of peaches (Red Heaven and Maria Blanca) versus nectarines (Big Top and Queen Giant) were recorded. In contrast, nectarines were characterized by higher hydroxycinnamates and dry matter. The apples’ cultivar Granny Smith exhibited a high level of titratable acidity (TA), while the Gala displayed a high level of soluble solids concentration (SSC), carotenoids, dry matter, hydroxycinnamic acids, and flavonols at the unpeeled fruit, whereas the Red Chief by increased anthocyanins, antioxidant capacity, total phenols, and flavonoids. Nashi pears with peel were more beneficial due to the strong skin contribution in the fruits’ beneficial compounds content. The peel of the Granny Smith cultivar was associated with an increased level of P, K, Ca, and Mg, whereas that of Red Chief with increased anthocyanins and Mg content.
Condoms, Trust and Stealthing: The Meanings Attributed to Unprotected Hetero-Sex
Authors: Alam Najiha , Alldred Pam
Published: April 2021

University students tend to have greater sexual health knowledge than the general public, yet condom use among this group continues to be a public health concern because effective condom use could reduce sexually transmitted infections and, for heterosexual women, unwanted pregnancies. We report findings from a small, qualitative study of condom use among sexually active heterosexual university students in the UK. In interviews, students shared their views about condom use and sometimes their personal experiences too. This paper identifies some of the meanings attributed to condom use in the accounts of nine heterosexually active 20–25 year-olds. Participants explained that when they felt comfortable communicating with their partners, they were more likely to use condoms, and those with negative sexual experiences or under social or psychological pressure were less likely to use them. The findings highlight issues of trust and power between men and women in heterosexual relationships, and describe contexts for dishonest sexual practice, including the traditional notions of femininity that were linked to condom use by this group. The issue of stealthing arose in one woman’s account of her experience and in several others’ reports of what occurs commonly. Stealthing, the secretive removal of a condom by a (usually male) partner during sexual intercourse without a partner’s knowledge or permission, produces non-consensual unprotected sex. We present stealthing as a product of the sexual double-standards described and as a form of interpersonal violence (IPV) and, among these heterosexual partners, as a form of gender-based violence. This study provides a glimpse into university students’ decision-making regarding condom use and highlights how gendered inequalities shape heterosex, in particular, communication about safer sex, that in some cases, compromise women’s decisions about (safer) sex.
Fungal and Bacterial Endophytes as Microbial Control Agents for Plant-Parasitic Nematodes
Authors: Kumar K. Kiran , Dara Surendra K.
Published: April 2021

Endophytes are symbiotic microorganisms that colonize plant tissues and benefit plants in multiple ways including induced systemic resistance to biotic and abiotic stresses. Endophytes can be sustainable alternatives to chemical nematicides and enhance plant health in a variety of cropping and natural environments. Several in vitro and in vivo studies demonstrated the potential of multiple species of Fusarium and Bacillus against plant-parasitic nematodes in horticultural, agricultural, and fodder crops and in forestry. While there were efforts to commercialize some of the endophytes as bionematicides, a lack of good formulations with consistent field efficacy has been a major hurdle in commercializing endophytes for nematode control. Identification of efficacious and environmentally resilient strains, a thorough understanding of their modes of action, interactions with various biotic and abiotic factors, and developing strategies that improve their effectiveness are critical areas to advance the commercialization of bionematicides based on fungal and bacterial endophytes.
Housing Spaces in Nine European Countries: A Comparison of Dimensional Requirements
Authors: Appolloni Letizia , D’Alessandro Daniela
Published: April 2021

Modern housing units must meet new needs and requirements; housing dimensions and functional characteristics are relevant issues, mainly considering population ageing and disability. The housing standards of nine European countries were compared to analyze their ability to satisfy new population need, in terms of size. The regulations were downloaded from the websites of the official channels of each country. A wide variability in room size was observed (e.g., single room: from 9 m2 in Italy to 7 m2 in France, to the absence of any limit in England and Wales, Germany-Hesse, and Denmark). Italian and French legislations define housing dimension considering the room destination and the number of people. The Swedish regulation provides performance requirements and functional indications but does not specify the minimum dimensions of habitable rooms. The rooms’ minimum heights vary between 2.70 m in Italy and Portugal and 2.60 m in the Netherlands, but no limits are established in England and Wales. A diverse approach among European countries regulations is observed: from a market-oriented logic one (e.g., England and Wales) in which room minimum dimensions are not defined to a prescriptive one (Italy) and one that is functionality-oriented (the Netherlands). However, considering the health, social, environmental, and economic trends, many of these standards should be revised.
Depressive Symptom Trajectories and Early Adult Education and Employment: Comparing Longitudinal Cohorts in Canada and the United States
Authors: Minh Anita , Bültmann Ute , Reijneveld Sijmen A. , van Zon Sander K. R. , McLeod Christopher B.
Published: April 2021

Adolescent depressive symptoms are risk factors for lower education and unemployment in early adulthood. This study examines how the course of symptoms from ages 16–25 influences early adult education and employment in Canada and the USA. Using data from the National Longitudinal Survey of Children and Youth (n = 2348) and the National Longitudinal Survey of Youth 79 Child/Young Adult (n = 3961), four trajectories (low-stable; increasing; decreasing; and increasing then decreasing, i.e., mid-peak) were linked to five outcomes (working with a post-secondary degree; a high school degree; no degree; in school; and NEET, i.e., not in employment, education, or training). In both countries, increasing, decreasing, and mid-peak trajectories were associated with higher odds of working with low educational credentials, and/or NEET relative to low-stable trajectories. In Canada, however, all trajectories had a higher predicted probability of either being in school or working with a post-secondary degree than the other outcomes; in the USA, all trajectory groups were most likely to be working with a high school degree. Higher depressive symptom levels at various points between adolescent and adulthood are associated with working with low education and NEET in Canada and the USA, but Canadians are more likely to have better education and employment outcomes.
Application of Artificial Intelligence-Based Regression Methods in the Problem of COVID-19 Spread Prediction: A Systematic Review
Authors: Musulin Jelena , Baressi Šegota Sandi , Štifanić Daniel , Lorencin Ivan , Anđelić Nikola , Šušteršič Tijana , Blagojević Anđela , Filipović Nenad , Ćabov Tomislav , Markova-Car Elitza
Published: April 2021

COVID-19 is one of the greatest challenges humanity has faced recently, forcing a change in the daily lives of billions of people worldwide. Therefore, many efforts have been made by researchers across the globe in the attempt of determining the models of COVID-19 spread. The objectives of this review are to analyze some of the open-access datasets mostly used in research in the field of COVID-19 regression modeling as well as present current literature based on Artificial Intelligence (AI) methods for regression tasks, like disease spread. Moreover, we discuss the applicability of Machine Learning (ML) and Evolutionary Computing (EC) methods that have focused on regressing epidemiology curves of COVID-19, and provide an overview of the usefulness of existing models in specific areas. An electronic literature search of the various databases was conducted to develop a comprehensive review of the latest AI-based approaches for modeling the spread of COVID-19. Finally, a conclusion is drawn from the observation of reviewed papers that AI-based algorithms have a clear application in COVID-19 epidemiological spread modeling and may be a crucial tool in the combat against coming pandemics.
Cost, Severity and Prevalence of Agricultural-Related Injury Workers’ Compensation Claims in Farming Operations from 14 U.S. States
Authors: Baidwan Navneet Kaur , Ramirez Marizen R. , Gerr Fred , Boonstra Daniel , Cavanaugh Joseph E. , Casteel Carri
Published: April 2021

(1) Background: There is no national surveillance of agricultural injuries, despite agricultural occupations being among the most hazardous in the U.S. This effort uses workers’ compensation (WC) data to estimate the burden of agricultural injuries and the likelihood of experiencing an injury by body part involved, cause, and nature in farming operations. (2) Methods: WC data from 2010 to 2016 provided by a large insurance company covering small to medium-sized farm operations from 14 U.S. states was used. We investigated the associations between injury characteristics and WC costs and the risk of having a more severe versus a less severe claim. The proportion of costs attributable to specific claim types was calculated. (3) Results: Of a total 1000 claims, 67% were medical only. The total cost incurred by WC payable claims (n = 866) was USD 21.5 million. Of this, 96% was attributable to more severe claims resulting in disabilities or death. The most common body part injured was the distal upper extremity. Falling or flying objects and collisions were the most expensive and common causes of injury. (4) Conclusions: Characterizing the cost and severity of agricultural injury by key injury characteristics may be useful when prioritizing prevention efforts in partnership with insurance companies and agricultural operations.
Concerns Related to the COVID-19 in Adult Norwegians during the First Outbreak in 2020: A Qualitative Approach
Authors: Skogstad Laila , Schou-Bredal Inger , Bonsaksen Tore , Heir Trond , Ekeberg Øivind , Grimholt Tine
Published: April 2021

Concerns related to the first outbreak of the COVID-19 pandemic in the Norwegian population are studied in a cross-sectional web-survey conducted between 8 April and 20 May 2020. The qualitative thematic analysis of the open-ended question “Do you have other concerns related to the pandemic?”, followed a six-step process. Concerns from 1491 informants were analyzed, 34% of women and 30% of men (p = 0.05) provided concerns. Respondents with higher educational level reported concerns more often (86% vs. 83%, p = 0.022). The qualitative analysis revealed five themes—society, health, social activities, personal economy and duration—and 13 sub-themes, mostly related to the themes “society” and “health” (724 and 704, respectively). Empathy for others was prominent, for society (nationally and globally), but also concerns related to infecting others and family members at risk for developing serious illness if infected. The responses to the open-ended question yielded additional information, beyond the information obtained from questions with pre-categorized response options, especially related to concerns about society and health. Themes arising from the qualitative analysis shed light on what are important concerns for people during the pandemic and this may serve as targeted measures for the authorities.
Association between Ambient Air Pollution and MRI-Defined Brain Infarcts in Health Examinations in China
Authors: Wu Jing , Ning Yi , Gao Yongxiang , Shan Ruiqi , Wang Bo , Lv Jun , Li Liming
Published: April 2021

The study aimed to evaluate the relationships between air pollutants and risk of magnetic resonance imaging (MRI)-defined brain infarcts (BI). We used data from routine health examinations of 1,400,503 participants aged ≥18 years who underwent brain MRI scans in 174 cities in 30 provinces in China in 2018. We assessed exposures to particulate matter (PM)2.5, PM10, nitrogen dioxide (NO2), and carbon monoxide (CO) from 2015 to 2017. MRI-defined BI was defined as lesions ≥3 mm in diameter. Air pollutants were associated with a higher risk of MRI-defined BI. The odds ratio (OR) (95% CI) for MRI-defined BI comparing the highest with the lowest tertiles of air pollutant concentrations was 2.00 (1.96–2.03) for PM2.5, 1.68 (1.65–1.71) for PM10, 1.58 (1.55–1.61) for NO2, and 1.57 (1.54–1.60) for CO. Each SD increase in air pollutants was associated with 16–42% increases in the risk of MRI-defined BI. The associations were stronger in the elderly subgroup. This is the largest survey to evaluate the association between air pollution and MRI-defined BI. Our findings indicate that ambient air pollution was significantly associated with a higher risk of MRI-defined BI.
Lymphopenia, Lymphopenia-Induced Proliferation, and Autoimmunity
Authors: Sheu Ting-Ting , Chiang Bor-Luen
Published: April 2021

Immune homeostasis is a tightly regulated system that is critical for defense against invasion by foreign pathogens and protection from self-reactivity for the survival of an individual. How the defects in this system might result in autoimmunity is discussed in this review. Reduced lymphocyte number, termed lymphopenia, can mediate lymphopenia-induced proliferation (LIP) to maintain peripheral lymphocyte numbers. LIP not only occurs in normal physiological conditions but also correlates with autoimmunity. Of note, lymphopenia is also a typical marker of immune aging, consistent with the fact that not only the autoimmunity increases in the elderly, but also autoimmune diseases (ADs) show characteristics of immune aging. Here, we discuss the types and rates of LIP in normal and autoimmune conditions, as well as the coronavirus disease 2019 in the context of LIP. Importantly, although the causative role of LIP has been demonstrated in the development of type 1 diabetes and rheumatoid arthritis, a two-hit model has suggested that the factors other than lymphopenia are required to mediate the loss of control over homeostasis to result in ADs. Interestingly, these factors may be, if not totally, related to the function/number of regulatory T cells which are key modulators to protect from self-reactivity. In this review, we summarize the important roles of lymphopenia/LIP and the Treg cells in various autoimmune conditions, thereby highlighting them as key therapeutic targets for autoimmunity treatments.
A Versatile Toolkit for Semi-Automated Production of Fluorescent Chemokines to Study CCR7 Expression and Functions
Authors: Artinger Marc , Matti Christoph , Gerken Oliver J. , Veldkamp Christopher T. , Legler Daniel F.
Published: April 2021

Chemokines guide leukocyte migration in different contexts, including homeostasis, immune surveillance and immunity. The chemokines CCL19 and CCL21 control lymphocyte and dendritic cell migration and homing to lymphoid organs. Thereby they orchestrate adaptive immunity in a chemokine receptor CCR7-dependent manner. Likewise, cancer cells that upregulate CCR7 expression are attracted by these chemokines and metastasize to lymphoid organs. In-depth investigation of CCR7 expression and chemokine-mediated signaling is pivotal to understand their role in health and disease. Appropriate fluorescent probes to track these events are increasingly in demand. Here, we present an approach to cost-effectively produce and fluorescently label CCL19 and CCL21 in a semi-automated process. We established a versatile protocol for the production of recombinant chemokines harboring a small C-terminal S6-tag for efficient and site-specific enzymatic labelling with an inorganic fluorescent dye of choice. We demonstrate that the fluorescently labeled chemokines CCL19-S6Dy649P1 and CCL21-S6Dy649P1 retain their full biological function as assessed by their abilities to mobilize intracellular calcium, to recruit β-arrestin to engaged receptors and to attract CCR7-expressing leukocytes. Moreover, we show that CCL19-S6Dy649P1 serves as powerful reagent to monitor CCR7 internalization by time-lapse confocal video microscopy and to stain CCR7-positive primary human and mouse T cell sub-populations.
Nucleocytoplasmic Transport: Regulatory Mechanisms and the Implications in Neurodegeneration
Authors: Ding Baojin , Sepehrimanesh Masood
Published: April 2021

Nucleocytoplasmic transport (NCT) across the nuclear envelope is precisely regulated in eukaryotic cells, and it plays critical roles in maintenance of cellular homeostasis. Accumulating evidence has demonstrated that dysregulations of NCT are implicated in aging and age-related neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS), frontotemporal dementia (FTD), Alzheimer’s disease (AD), and Huntington disease (HD). This is an emerging research field. The molecular mechanisms underlying impaired NCT and the pathogenesis leading to neurodegeneration are not clear. In this review, we comprehensively described the components of NCT machinery, including nuclear envelope (NE), nuclear pore complex (NPC), importins and exportins, RanGTPase and its regulators, and the regulatory mechanisms of nuclear transport of both protein and transcript cargos. Additionally, we discussed the possible molecular mechanisms of impaired NCT underlying aging and neurodegenerative diseases, such as ALS/FTD, HD, and AD.
Adenovirus-Mediated Inducible Expression of a PD-L1 Blocking Antibody in Combination with Macrophage Depletion Improves Survival in a Mouse Model of Peritoneal Carcinomatosis
Authors: Buñuales Maria , Ballesteros-Briones Maria Cristina , Gonzalez-Aparicio Manuela , Hervas-Stubbs Sandra , Martisova Eva , Mancheño Uxua , Ricobaraza Ana , Lumbreras Sara , Smerdou Cristian , Hernandez-Alcoceba Ruben
Published: April 2021

Immune checkpoint inhibitors (ICIs) have demonstrated remarkable efficacy in a growing number of malignancies. However, overcoming primary or secondary resistances is difficult due to pharmacokinetics issues and side effects associated with high systemic exposure. Local or regional expression of monoclonal antibodies (mAbs) using gene therapy vectors can alleviate this problem. In this work, we describe a high-capacity adenoviral vector (HCA-EFZP-aPDL1) equipped with a mifepristone-inducible system for the controlled expression of an anti-programmed death ligand 1 (PD-L1) blocking antibody. The vector was tested in an immune-competent mouse model of colorectal cancer based on implantation of MC38 cells. A single local administration of HCA-EFZP-aPDL1 in subcutaneous lesions led to a significant reduction in tumor growth with minimal release of the antibody in the circulation. When the vector was tested in a more stringent setting (rapidly progressing peritoneal carcinomatosis), the antitumor effect was marginal even in combination with other immune-stimulatory agents such as polyinosinic-polycytidylic acid (pI:C), blocking mAbs for T cell immunoglobulin, mucin-domain containing-3 (TIM-3) or agonistic mAbs for 4-1BB (CD137). In contrast, macrophage depletion by clodronate liposomes enhanced the efficacy of HCA-EFZP-aPDL1. These results highlight the importance of addressing macrophage-associated immunoregulatory mechanisms to overcome resistance to ICIs in the context of colorectal cancer.
Genetics of Cardiovascular Disease: How Far Are We from Personalized CVD Risk Prediction and Management?
Authors: Vrablik Michal , Dlouha Dana , Todorovova Veronika , Stefler Denes , Hubacek Jaroslav A.
Published: April 2021

Despite the rapid progress in diagnosis and treatment of cardiovascular disease (CVD), this disease remains a major cause of mortality and morbidity. Recent progress over the last two decades in the field of molecular genetics, especially with new tools such as genome-wide association studies, has helped to identify new genes and their variants, which can be used for calculations of risk, prediction of treatment efficacy, or detection of subjects prone to drug side effects. Although the use of genetic risk scores further improves CVD prediction, the significance is not unambiguous, and some subjects at risk remain undetected. Further research directions should focus on the “second level” of genetic information, namely, regulatory molecules (miRNAs) and epigenetic changes, predominantly DNA methylation and gene-environment interactions.
Genetic Variants in Transcription Factor Binding Sites in Humans: Triggered by Natural Selection and Triggers of Diseases
Authors: Tseng Chia-Chun , Wong Man-Chun , Liao Wei-Ting , Chen Chung-Jen , Lee Su-Chen , Yen Jeng-Hsien , Chang Shun-Jen
Published: April 2021

Variants of transcription factor binding sites (TFBSs) constitute an important part of the human genome. Current evidence demonstrates close links between nucleotides within TFBSs and gene expression. There are multiple pathways through which genomic sequences located in TFBSs regulate gene expression, and recent genome-wide association studies have shown the biological significance of TFBS variation in human phenotypes. However, numerous challenges remain in the study of TFBS polymorphisms. This article aims to cover the current state of understanding as regards the genomic features of TFBSs and TFBS variants; the mechanisms through which TFBS variants regulate gene expression; the approaches to studying the effects of nucleotide changes that create or disrupt TFBSs; the challenges faced in studies of TFBS sequence variations; the effects of natural selection on collections of TFBSs; in addition to the insights gained from the study of TFBS alleles related to gout, its associated comorbidities (increased body mass index, chronic kidney disease, diabetes, dyslipidemia, coronary artery disease, ischemic heart disease, hypertension, hyperuricemia, osteoporosis, and prostate cancer), and the treatment responses of patients.
Genetic Neonatal-Onset Epilepsies and Developmental/Epileptic Encephalopathies with Movement Disorders: A Systematic Review
Authors: Spagnoli Carlotta , Fusco Carlo , Percesepe Antonio , Leuzzi Vincenzo , Pisani Francesco
Published: April 2021

Despite expanding next generation sequencing technologies and increasing clinical interest into complex neurologic phenotypes associating epilepsies and developmental/epileptic encephalopathies (DE/EE) with movement disorders (MD), these monogenic conditions have been less extensively investigated in the neonatal period compared to infancy. We reviewed the medical literature in the study period 2000–2020 to report on monogenic conditions characterized by neonatal onset epilepsy and/or DE/EE and development of an MD, and described their electroclinical, genetic and neuroimaging spectra. In accordance with a PRISMA statement, we created a data collection sheet and a protocol specifying inclusion and exclusion criteria. A total of 28 different genes (from 49 papers) leading to neonatal-onset DE/EE with multiple seizure types, mainly featuring tonic and myoclonic, but also focal motor seizures and a hyperkinetic MD in 89% of conditions, with neonatal onset in 22%, were identified. Neonatal seizure semiology, or MD age of onset, were not always available. The rate of hypokinetic MD was low, and was described from the neonatal period only, with WW domain containing oxidoreductase (WWOX) pathogenic variants. The outcome is characterized by high rates of associated neurodevelopmental disorders and microcephaly. Brain MRI findings are either normal or nonspecific in most conditions, but serial imaging can be necessary in order to detect progressive abnormalities. We found high genetic heterogeneity and low numbers of described patients. Neurological phenotypes are complex, reflecting the involvement of genes necessary for early brain development. Future studies should focus on accurate neonatal epileptic phenotyping, and detailed description of semiology and time-course, of the associated MD, especially for the rarest conditions.