bluuebunny/crossref_metadata_2025 huggingface dataset:
Leveraging Delta Smelt Data for Juvenile Chinook Salmon Monitoring in the San Francisco Estuary
Authors: Mahardja Brian , Mitchell Lara , Beakes Michael , Johnston Catherine , Graham Cory , Goertler Pascale , Barnard Denise , Castillo Gonzalo , Matthias Bryan
Published: June 2020

Monitoring is an essential component in ecosystem management and leveraging existing data sources for multiple species of interest can be one effective way to enhance information when making management decisions. Here we analyzed juvenile Chinook Salmon (Oncorhynchus tshawytscha) bycatch data that has been collected by the recently established Enhanced Delta Smelt Monitoring program (EDSM), a survey designed to estimate the abundance and distribution of the San Francisco Estuary (estuary) endemic and endangered Delta Smelt (Hypomesus transpacificus). Two key aspects of the EDSM program distinguish it from other fish surveys in the estuary: a stratified random sampling design and the spatial scale of its sampling effort. We integrated the EDSM dataset with other existing surveys in the estuary and used an occupancy model to assess detection probability differences across gear types. We saw no large-scale differences in size selectivity, and while detection probability varied among gear types, cumulative detection probability for EDSM was comparable to other surveys due to the program’s use of replicate tows. Based on our occupancy model and sampling effort in the estuary during spring of 2017 and 2018, we highlighted under-sampled regions that saw improvements in monitoring coverage due to EDSM. Our analysis also revealed that each sampling method has its own benefits and constraints. Although the use of random sites with replicates as conducted by EDSM can provide more statistically robust abundance estimates relative to traditional methods, the use of fixed stations and simple methods such as beach seine may provide a more cost-effective way of monitoring salmon occurrence at certain regions of the estuary. Stronger inference on salmon abundance and distribution can be made by leveraging the strengths of each survey’s method. Careful consideration of these trade-offs and key monitoring objectives is crucial as the management agencies of the estuary continue to adapt and improve their monitoring programs.
Reform in Upper Secondary School Governance Towards Autonomy Model: A Case Study of Reality Autonomy Model in Vietnam
Authors: Huong Nguyen Thi , Phuong Pham Thu
Published: June 2020

In Vietnam, general education includes primary education, lower secondary education (the period of basic education) and upper secondary education (the period of vocational orientation education). In particular, primary education is compulsory for all children from 6 to 14 years old, is implemented in 5 school years, from first grade to fifth grade. The age of students entering first grade is six years old. Primary education aims to help students form the initial foundations for proper and long-term development of morality, intelligence, physicality, aesthetics and basic skills for students to continue high school. Secondary education is conducted in four school years, from grade six to grade nine. Students entering sixth grade must have an elementary school diploma. Secondary education aims to help students consolidate and develop the results of primary education; have basic secondary education and initial knowledge about technology and career to continue high school, vocational high school, vocational training or enter a working life. High school education is conducted in 3 school years, from grade ten to grade twelve. Students entering tenth grade must have a junior high school diploma. High school education is aimed at helping students consolidate and develop the outcomes of lower secondary education, complete high school education and common knowledge about technology and career guidance for further college education, college, professional secondary school, apprenticeship or enter the working life. Thus, the term general education is a term with broad connotation including primary education, lower secondary education (basic education period) and upper secondary education (education period) career orientation. Within the scope of this research, we use the term school governance used to mean a mode of action, which is directed toward the goal of being accomplished effectively, by and through others. Governance activities are indispensable activities that arise when people work together to accomplish goals.
Моделирование упругих свойств композитных материалов методом асимптотического осреднения с учетом неидеального интерфейса компонент
Authors: Соколов Александр Павлович , Sokolov Alexander Pavlovich , Щетинин Виталий Николаевич , Schetinin Vitalii Nikolaevich , Козлов Михаил Юрьевич , Kozlov Mikhail Yur'evich
Published: June 2020

Представлена модификация метода асимптотического осреднения для решения задачи гомогенизации упругих свойств композитных материалов с учетом упругости
интерфейса между фазами. Рассматриваются условия мягкого неидеального интерфейса, допускающие скачок поля перемещений при переходе через границу
фаз. Предложен переход от модели композита с тонким межфазным слоем к модели с неидеальным интерфейсом. Представлен литературный обзор методов моделирования интерфейса между матрицей и наполнителем. Для численной реализации метода осреднения используется метод конечных элементов. Предложена
модель поверхностного интерфейсного конечного элемента, реализующего скачок
поля перемещений при переходе через границу фаз. Численный метод осреднения
упругих свойств адаптируется к наличию разрыва поля перемещений. Оцениваются пороговые значения относительной жесткости и толщины межфазного слоя, для
которых применимо моделирование с помощью условий скачка перемещений.
Ставится задача идентификации параметров интерфейса по экспериментально полученным техническим константам композита. Вычислительные эксперименты
проводятся для дисперсно-армированного и однонаправленного композита с изотропным включением.
The Anatomy and Origin of a Synconvergent Grenvillian-Age Metamorphic Core Complex, Chottanagpur Gneiss Complex, Eastern India
Authors: Sequeira Nicole , Mahato Souradeep , Rahl Jeffrey M. , Sarkar Soumendu , Bhattacharya Abhijit
Published: June 2020

Amphibolite facies supracrustal rocks interleaved with granite mylonites constitute a shallowly dipping carapace overlying granulite facies anatectic basement gneisses in the Giridih-Dumka-Deoghar-Chakai area that spans ~11,000 km2 in the Chottanagpur Gneiss Complex (CGC). Steep N-trending tectonic fabrics in the gneisses include recumbent folds adjacent to the overlying carapace. The basement and carapace are dissected by steep-dipping sinistral shear zones with shallow/moderately plunging stretching lineations. The shear zones trend NNE in the north (north-down kinematics) and ESE in the south (south-down kinematics). Chemical ages in metamorphic monazites in the lithodemic units are overwhelmingly Grenvillian in age (1.0–0.9 Ga), with rafts of older domains in the basement gneisses (1.7–1.45 Ga), granitoids (1.4–1.3 Ga), and the supracrustal rock (1.2–1.1 Ga). P-T pseudosection analysis indicates the supracrustal rocks within the carapace experienced postthrusting midcrustal heating (640–690°C); the Grenvillian-age P-T path is distinct from the existing Early Mesoproterozoic P-T path reconstructed for the basement gneisses. Quartz opening angle thermometry indicates that high temperature (~600°C) persisted during deformation in the southern shear zone. Kinematic vorticity values in carapace-hosted granitoid mylonites and in steep-dipping shear zones suggest transpressional deformation involved a considerable pure shear component. Crystallographic vorticity axis analysis also indicates heterogeneous deformation, with some samples recording a triclinic strain. The basement-carapace composite was extruded along an inclined channel bound by the steep left-lateral transpressional shear zones. Differential viscous extrusion during crustal shortening coupled with the collapse of the thickened crust caused midcrustal flow along flat-lying detachments in the carapace.
Pulsed Mesozoic Deformation in the Cordilleran Hinterland and Evolution of the Nevadaplano: Insights from the Pequop Mountains, NE Nevada
Authors: Zuza Andrew V. , Thorman Charles H. , Henry Christopher D. , Levy Drew A. , Dee Seth , Long Sean P. , Sandberg Charles A. , Soignard Emmanuel
Published: June 2020

Mesozoic crustal shortening in the North American Cordillera’s hinterland was related to the construction of the Nevadaplano orogenic plateau. Petrologic and geochemical proxies in Cordilleran core complexes suggest substantial Late Cretaceous crustal thickening during plateau construction. In eastern Nevada, geobarometry from the Snake Range and Ruby Mountains-East Humboldt Range-Wood Hills-Pequop Mountains (REWP) core complexes suggests that the ~10–12 km thick Neoproterozoic-Triassic passive-margin sequence was buried to great depths (>30 km) during Mesozoic shortening and was later exhumed to the surface via high-magnitude Cenozoic extension. Deep regional burial is commonly reconciled with structural models involving cryptic thrust sheets, such as the hypothesized Windermere thrust in the REWP. We test the viability of deep thrust burial by examining the least-deformed part of the REWP in the Pequop Mountains. Observations include a compilation of new and published peak temperature estimates (n=60) spanning the Neoproterozoic-Triassic strata, documentation of critical field relationships that constrain deformation style and timing, and new 40Ar/39Ar ages. This evidence refutes models of deep regional thrust burial, including (1) recognition that most contractional structures in the Pequop Mountains formed in the Jurassic, not Cretaceous, and (2) peak temperature constraints and field relationships are inconsistent with deep burial. Jurassic deformation recorded here correlates with coeval structures spanning western Nevada to central Utah, which highlights that Middle-Late Jurassic shortening was significant in the Cordilleran hinterland. These observations challenge commonly held views for the Mesozoic-early Cenozoic evolution of the REWP and Cordilleran hinterland, including the timing of contractional strain, temporal evolution of plateau growth, and initial conditions for high-magnitude Cenozoic extension. The long-standing differences between peak-pressure estimates and field relationships in Nevadan core complexes may reflect tectonic overpressure.
Healthy adult vegetarians have better renal function than matched omnivores: a cross sectional study in China.
Authors: Xu Kaijie , Cui Xueying , Wang Bian , Tang Qingya , Cai Jianfang , Shen Xiuhua
Published: June 2020

Background: An appropriate diet is an important determinant of kidney health. However, the association between vegetarian diets and renal function is unclear. We aimed to study the association between vegetarian diets and renal function in healthy adults. Methods: A total of 269 vegetarians and 269 sex- and age-matched nonvegetarian omnivores were enrolled in this cross-sectional study. Basic characteristics and daily dietary intakes were assessed by face-to-face interviews. Blood samples were collected, and renal function was assessed by measuring blood urea nitrogen (BUN), serum creatinine (SCr), uric acid (UA) and the estimated glomerular filtration rate (eGFR). Blood pressure, fasting blood glucose and blood lipid profiles were also assessed.Results: The average age of the vegetarians was 35.4±8.6 years, 82.2% of whom were female. We evaluated the association between vegetarian diets and renal function using multivariate analysis. Compared with omnivores, vegetarians had lower BUN [β= -0.63, 95% confidence interval (CI): (-0.88 , -0.38)], SCr [β= -2.04, 95% CI:(-4.10, 0.02)], and UA levels [β= -15.15, 95% CI: (-27.81, -2.50)] and higher eGFRs [β= 4.04, 95% CI: (0.30, 7.78)] after adjusting for sex, age, body mass index (BMI), physical activity, alcohol consumption, smoking status, low-density lipoprotein cholesterol (LDL), high-density lipoprotein cholesterol (HDL), systolic pressure and fasting blood glucose. Further analysis of food composition and renal function showed that dietary fiber intake was significantly negatively associated with BUN [β= -0.02, 95% CI: (-0.03, 0.00)], SCr [β= -0.14, 95% CI: (-0.25, 0.04)], and UA levels [β= -0.72, 95% CI: (-1.36, 0.07)] and positively associated with the eGFR [β= 0.20, 95% CI: (0.00, 0.40)]. Conclusions: Healthy adult vegetarians have better renal function than omnivores, and the higher dietary fiber intake associated with vegetarian diets may contribute to the protective effect on renal function.
A comprehensive study on the oncogenic mutation and molecular pathology in Chinese lung adenocarcinoma patients
Authors: Zhang Xilin , Jiang Yan , Yu Huanming , Xia Hui , Wang Xiang
Published: June 2020

Background: Several genetic driver alterations have been identified in micropapillary lung adenocarcinoma (MPA). However, the frequency of co-alteration of ROS1, EGFR and EML4-ALK is yet unclear. Herein, we investigated the relationship between clinicopathologic characteristics and well-identified driver mutations of MPA compared with non-micropapillary lung adenocarcinoma (LA). Methods: Formalin fixed paraffin-embedded (FFPE) sections derived from lung adenocarcinoma patients who never received adjuvant chemotherapy or radiation therapy prior to surgical resection were collected from October 2016 to June 2019. EGFR mutations, ROS1 rearrangements and EML4-ALK fusion were identified in a set of 131 MPA and LA cases by using the Amplification Refractory Mutation System (ARMS). The response rate and duration of response were assessed using Response Evaluation Criteria in Solid Tumors version 1.1 (RECIST 1.1). Results: EGFR mutations had occurred in 42 (76.4%) MPA patients and 42 (55.3%) LA patients. Interestingly, ROS1 rearrangements were highly enriched only in the MPA cases (6/55, 10.9%) but rarely in the LA cases (1/76, 1.3%). Furthermore, 7.3% (4/55) MPA samples had double gene mutations, while only 1.3% (1/76) LA cases had double gene alterations. Of 5 patients with harboring two driver oncogenes mutations, four patients (80%) obtained partial response and one patient (20%) suffered recurrence. Conclusions: A higher prevalence of ROS1 rearrangement or combined mutations of ROS1, EGFR and EML4-ALK may play a critical role in the tumorigenesis of MPA. These findings provide a novel therapeutic strategy for patients with malignant MPA through combining TKIs than one TKI.
Prevalence and Predictors of Anxiety and Depressive Symptoms among Patients Diagnosed with Oral Cancer in China: A cross-sectional study
Authors: Yuan Lulu , Pan Bochen , Wang Weiren , Wang Lie , Zhang Xujie , Gao Yuqin
Published: June 2020

Background
Anxiety and depression are common mental health problems among patients with cancer. While many psychological variables have been proven to influence anxiety and depressive symptoms, the variables are not mutually exclusive and their integrated effects on patients with oral cancer are yet unknown. The present study aims to explore the prevalence of anxiety and depressive symptoms among patients with oral cancer, to find out key potentially predictive factors associated with anxiety and depressive symptoms.
Method
A cross-sectional study was carried out for Chinese patients with oral cancer between May 2016 and October 2017 in two Grade-A Tertiary Hospitals in Shenyang, China. 230 patients with oral cancer were interviewed with questionnaires on demographic variables, Zung Self-Rating Anxiety Scale (SAS), Center for Epidemiologic Studies Depression Scale (CES-D), Herth Hope Index (HHI), Social Impact Scale, Multidimensional Scale of Perceived Social Support (MSPSS), Revised Life Orientation Test (LOT-R), Perceived Stress Scale-10 (PSS-10), and General Perceived Self-efficacy Scale(GSE). Chi-square test, nonparametric test, t-test and logistic regression analyses were conducted where appropriate to explore predictive factors of anxiety symptoms and depressive symptoms.
Result
The prevalence of anxiety symptoms and depressive symptoms in the sample population was 36.96% (85/230) and 65.21% (150/230), respectively. Social isolation dimension of stigma (β=0.436, OR=1.547, CI:1.211~1.975), optimism (β=-0.276, OR=0.759, CI:0.624~0.922), and perceived stress (β=0.217, OR=1.243, CI:1.092~1.414) were predictors of anxiety symptoms. Marriage (β=1.648, OR=5.198, CI:1.427~18.924), positive readiness and expectancy dimension of hope (β=-0.505, OR=0.604, CI:0.395~0.923), social isolation dimension of stigma (β=0.314, OR=1.368, CI:1.054~1.776) and perceived stress (β=0.273, OR=1.314, CI:1.134~1.524) were predictors of depressive symptoms among oral cancer patients.
Conclusion
The prevalence of anxiety symptoms and depressive symptoms was high among oral cancer patients in China. The communal predictors of anxiety and depressive symptoms in patients with oral cancer were levels of perceived stress and social isolation of stigma. In addition, optimism was a predictor of anxiety symptoms and hope was a predictor of depressive symptoms.
Association between nonalcoholic fatty liver disease and extrahepatic cancers: A systematic review and meta-analysis
Authors: Liu Shou-Sheng , Ma Xue-Feng , Zhao Jie , Du Shui-Xian , Zhang Jie , Dong Meng-Zhen , Xin Yong-Ning
Published: June 2020

Background NAFLD is tightly associated with various diseases such as diabetes, cardiovascular disease, kidney disease, and cancer. Previous studies had investigated the association between NAFLD and various extrahepatic cancers, but the available data to date is not conclusive. The aim of this study was to investigate the association between NAFLD and various extrahepatic cancers comprehensively. Methods Searches were conducted of various electronic databases (PubMed, EMBASE, Medline, and the Cochrane Library) to identify observational studies published between 1996 and January 2020 which investigated the association between NAFLD and extrahepatic cancers. The pooled OR/HR/IRR of the association between NAFLD and various extrahepatic cancers were analyzed. Results A total of 26 studies were included to investigate the association between NAFLD and various extrahepatic cancers. As the results shown, the pooled OR values of the risk of colorectal cancer and adenomas in patients with NAFLD were 1.72 (95%CI: 1.40-2.11) and 1.37 (95%CI: 1.29-1.46), respectively. The pooled OR values of the risk of intrahepatic cholangiocarcinoma and extrahepatic cholangiocarcinoma in patients with NAFLD were 2.46 (95%CI: 1.77-3.44) and 2.24 (95%CI: 1.58-3.17), respectively. The pooled OR value of the risk of breast cancer in patients with NAFLD was 1.69 (95%CI: 1.44-1.99). In addition, NAFLD was also tightly associated with the risk of gastric cancer, pancreatic cancer, prostate cancer, and esophageal cancer. Conclusions NAFLD could significantly increase the development risk of colorectal adenomas and cancer, intrahepatic and extrahepatic cholangiocarcinoma, breast, gastric, pancreatic, prostate, and esophageal cancer. NAFLD could be considered as one of the influencing factors during the clinical diagnosis and treatment for the extrahepatic cancers.
Early essential newborn care is associated with increased breastfeeding: a quasi-experimental study from Sichuan Province of western China
Authors: Wang Chenran , Li Xiayun , Zhang Lin , Wu Linman , Tan Ling , Yuan Fei , Guo Yao , Williams Sarah , Xu Tao
Published: June 2020

Background: Breastfeeding is critical to promote maternal and child health in a short and long term. China has set national targets to further improve the exclusive breastfeeding rate. We aimed to examine associations between the provision of early essential newborn care (EENC) and breastfeeding outcomes among full-term vaginally delivered neonates in the first six months of life. Methods: We conducted a quasi-experimental study in eight maternal and children’s hospitals in Mianyang City and Deyang City in Sichuan Province of western China. Four hospitals were randomly selected as the intervention group with the implementation of EENC while others as the control group receiving routine care. We assessed effects of EENC on breastfeeding initiation time, duration of first-time breastfeeding, and exclusive breastfeeding rates up to six months of age. Data in both groups were collected after delivery, at hospital discharge, 1 month, 3 months, and 6 months post birth in the baseline phase from May to June 2017 and post-EENC phase from October to December 2017. Written consent was obtained from eligible mothers enrolled in this study. We performed univariate analyses to ascertain differences between the two groups, and difference in difference (DID) models to explore the net effects. Results: Of the 1349 enrolled mother and newborn pairs in our study, 1131 (83.9%) were followed up at 1 month of age, 1075 (79.7%) at 3 months, and 981 (72.7%) at 6 months. EENC was associated with earlier median time to initiate breastfeeding (25min vs. 33min, P<0.01), an increased chance of successful first-time breastfeeding (OR=5.53; 95% CI: 2.69, 11.40), longer duration of skin to skin contact (SSC) (21.53 min; 95% CI: 18.17, 24.89) and longer duration of the first breastfeed (4.16 min; 95% CI: 2.10, 6.22), and an increased likelihood of being exclusively breastfed at discharge (74.5% vs. 55.0%, P<0.001), 3 months (OR=3.20; 95% CI: 1.01, 10.15), and 6 months (OR=4.91; 95% CI: 1.71, 14.13) of age. Conclusions: EENC enhances early and successful breastfeeding initiation, prolongs duration of the first breastfeed, and increases the rate of exclusive breastfeeding at six months of age. Our evidence suggests that nation wide scale up of EENC would increase the exclusive breastfeeding rate in the first six months of life.
Efficacy and safety of intraarticular corticosteroid injections in adolescents with juvenile idiopathic arthritis in the temporomandibular joint: A Norwegian 2-year prospective multicenter pilot study.
Authors: Frid Paula , Augdal Thomas A. , Larheim Tore A. , Halbig Josefine , Rypdal Veronika , Songstad Nils Thomas , Rosen Annika , Tylleskär Karin B. , Berstad Johanna Rykke , Flatø Berit , Stoustrup Peter , Rosendahl Karen , Kirkhus Eva , Nordal Ellen
Published: June 2020

Background. Intraarticular corticosteroids (IACs) have been used to treat temporomandibular joint (TMJ) arthritis. However, prospective clinical studies with magnetic resonance imaging (MRI) scoring are lacking. The aim of this study was to examine efficacy and safety of a single IAC in the TMJ in adolescents with juvenile idiopathic arthritis (JIA) in a clinical setting. Methods. In this Norwegian prospective multicenter pilot study 15 patients with JIA (mostly persistent oligo-arthritis or RF negative polyarthritis categories) and a clinically and MRI-verified diagnosis of TMJ arthritis were treated with IACs and followed for two years. Demographics, systemic medication, general disease activity and outcome measures were recorded including a pain-index score and maximal incisal opening (MIO). Inflammation and bone damage scores were assessed, using two recently published MRI scoring systems with masked radiological evaluation.Results. Among the 15 patients, 13 received a single IAC (5 bilateral), and 2 repeated IACs once unilaterally. Thus, the total number of IACs was 22. Median age was 15 years and the majority had an age not thought of as critical regarding mandibular growth retardation due to steroid injection. During the 2-year observation period systemic medication with disease modifying antirheumatic drugs (DMARDs) including biologics was initiated or adjusted in 10/15 (67%) patients. At the 2-months study visit after injection we observed a minimal improvement in MIO from median 44 (1st, 3rd quartiles; 36, 48) mm to 45 (43, 47) mm, p= 0.045 and decreased MRI mean additive inflammatory score from 4.4±1.8 standard deviations (SD) to 3.4±2.0, p= 0.040. From baseline to the 2-months follow-up pain improved in 6/11 patients but pain scores were not significantly improved. MRI-assessed damage increased in two patients with repeated IACs, and decreased in 3 patients but most of the patients were stable over the 2-year follow-up. Intra-rater repeatability of the MRI scoring system domains varied from poor to excellent.Conclusions. In this pilot study of predominately single IACs to the TMJ in combination with systemic treatment we observed improvement in MRI-assessed inflammation, mostly stable condylar bone conditions and minimal clinical improvement in adolescents with JIA and TMJ arthritis. No severe side effects were seen.
Intravenous Calcium as a Pressor in a Swine Model of Hypoxic Pseudo-Pulseless Electrical Mechanical Activity - A Preliminary Report
Authors: Lindqwister Alexander , Lampe Joshua W. , Gould Jeffrey R. , Kaufman Christopher L. , Moodie Karen , Paradis Norman
Published: June 2020

Background: Pseudo-pulseless electrical activity (pseudo-PEA) is a lifeless form of profound cardiac shock characterized by measurable cardiac mechanical activity without clinically detectable pulses. Pseudo-PEA may constitute up to 40% of reported cases of cardiac arrest. Resuscitation from pseudo-PEA is often associated with hypotension refractory to catecholamine pressors. We hypothesized that this post-resuscitation state may be associated with hypocalcemic hypotension responsive to intravenous calcium. Methods: Using pre-existing data from our hypoxic swine pseudo-PEA model, we measured blood pressure, hemodynamics, and electrolytes. Physiological data were analyzed on a heartbeat by heartbeat basis. The midpoint of the calcium response was defined using change of curvature feature detection. Hemodynamic parameters were shifted such that the value at the midpoint was equal to zero. Results: In 9 animals with refractory hypotension we administered 37 boluses of intravenous calcium in the dosage range of 5 -20 mg. Comparisons were made between the average values in the time period 40-37 seconds before the midpoint and 35-40 seconds after the midpoint. Of the 37 administered boluses, 34 manifested a change in the blood pressure, with mean aortic pressure, systolic and diastolic pressures all increasing post bolus administration. Conclusions: Administration of intravenous calcium may be associated with a pressor-like response in refractory hypotension after resuscitation from pseudo-PEA. Relative ionized hypocalcemia may cause hypotension after resuscitation from pseudo-PEA. Therapy with intravenous calcium should be further investigated in this setting.
Effects of oral contraceptives on the quality of life of women with polycystic ovary syndrome: a crossover randomized controlled trial
Authors: Amiri Mina , Nahidi Fatemeh , Bidhendi-Yarandi Razieh , Khalili Davood , Tohidi Maryam , Tehrani Fahimeh Ramezani
Published: June 2020

Background and objective A limited number of studies have evaluated the effects of oral contraceptives (OCs) on the quality of life (QOL) of polycystic ovary syndrome (PCOS) patients. This study aimed to compare the effects of using OCs containing levonorgestrel (LNG) and those containing desogestrel (DSG), cyproterone acetate (CPA) or drospirenone (DRSP) for 6 months on the QOL with PCOS. Methods In this crossover randomized controlled 6-arm trial, 200 eligible patients with PCOS scheduled for OC therapy were randomly assigned to one of the 6 study arms. All 6 arms include two 6-month treatment periods, one period with OCs containing LNG, and the other with each of the 3 OCs containing DSG, CPA, or DRSP. Outcomes of interest were the total score of QOL and its domains, which were assessed using a specific and valid health‐related quality of life questionnaire for PCOS, which is consisted of six domains, including psychosocial–emotional, self-image, fertility, sexual function, hirsutism, and obesity– menstrual disorders.Results Finally, a total of 88 patients were analyzed for this study. The results showed that use of OCs containing DSG, CPA, and DRSP for 3 months was not associated with significant differences in the total scores of QOL compared to those OCs containing LNG, whereas, after 6 months of treatment, patients treated with OCs containing CPA had more improvements in their total scores of QOL, in comparison to OCs containing LNG (P < 0.042). We found no significant differences in QoL domains, including psychosocial–emotional, self-image, fertility, sexual function, hirsutism, and obesity-menstrual disorders after 3- 6 months of treatment with DSG, CPA, or DRSP, compared to LNG. The sequence and period effects were not significant in any of the analyses at 3 and 6 months of treatment. The carry-over effect was not significant for most outcomes assessed. Conclusions This crossover study demonstrated non-inferiority of OCs with newer generation progestins on different domains of QOL, in comparison with older compounds, although usage of products containing CPA was significantly associated with more improvement in total QOL of PCOS patients, compared to those containing LNG after 6-month of treatment. Clinical Trial Registration Number: IRCT201702071281N2.
Metabolic engineering of phosphite metabolism in Synechococcus elongatus PCC 7942 as an effective measure to control biological contaminants in outdoor raceway ponds
Authors: González-Morales Sandra Isabel , Pacheco-Gutiérrez Navid , Ramírez-Rodríguez Carlos A. , Brito-Bello Alethia A. , Estrella-Hernández Priscila , Herrera-Estrella Luis , ARREDONDO DAMAR LOPEZ
Published: June 2020

Background: The use of cyanobacteria and microalgae as cell factories to produce biofuels and added-value bioproducts has received great attention during the last two decades. Important investments have been made by public and private sectors to develop this field. However, it has been a challenge to develop a viable and cost-effective platform for cultivation of cyanobacteria and microalgae under outdoor conditions. Dealing with contamination caused by bacteria, weedy algae/cyanobacteria and other organisms is a major constraint to establish effective cultivation processes.Results: Here we describe the implementation in the cyanobacterium Synechococcus elongatus PCC 7942 of a phosphorus selective nutrition system to control biological contamination during cultivation. The system is based on metabolic engineering of S. elongatus to metabolize phosphite, a phosphorus source not normally metabolized by most organisms, by expressing a bacterial phosphite oxidoreductase (PtxD). Engineered S. elongatus strains expressing PtxD grow at a similar rate on media supplemented with phosphite as the non-transformed control supplemented with phosphate. We show that when grown in media containing phosphite as the sole phosphorus source in glass flasks, the engineered strain was able to grow and outcompete biological contaminants even when the system was intentionally inoculated with natural competitors isolated from an irrigation canal. The PtxD/phosphite system was successfully used for outdoor cultivation of engineered S. elongatus in 100 L cylindrical reactors and 1,000 L raceway ponds, under non-axenic conditions and without the need of sterilizing containers and media. Finally, we also show that the PtxD/phosphite system can be used as selectable marker for S. elongatus PCC 7942 transgenic strains selection, eliminating the need of antibiotic resistance genes. Conclusions: Our results suggest that the PtxD/phosphite system is a stable and sufficiently robust strategy to control biological contaminants without the need of sterilization or other complex aseptic procedures. Our data shows that the PtxD/phosphite system can be used as selectable marker and allows production of the cyanobacterium S. elongatus PCC 7942 in non-axenic outdoor reactors at lower cost, which in principle should be applicable to other cyanobacteria and microalgae engineered to metabolize phosphite.
Tsetse fly distribution and occurrence of Trypanosoma species among cattle and goats around Queen Elizabeth National park, Uganda
Authors: Kangume Mallion , Muhangi Denis , Byaruhanga Joseph , Agaba Aggrey , Sserunkuma Joachim , Kisembo Stallon Justus , Bogere Paul , Vudriko Patrick , Rwego Innocent Bidason
Published: June 2020

Background

African Animal Trypanosomiasis (AAT) is an infectious disease of economic and public health importance hindering agricultural productivity in Sub-Saharan Africa. The current study aimed at providing baseline information on tsetse fly distribution and occurrence of Trypanosoma species in cattle and goats within and around Queen Elizabeth National Park (QENP), in western Uganda. A minimal entomological survey was conducted in April 2017 while blood samples collected from cattle (n = 576) and goats (n = 319) in June 2015 and May 2017 were subjected to microscopy and Polymerase Chain Reaction (PCR) to determine the occurrence of trypanosome species.
Results

Glossina pallidipes and G. fuscipes were the only tsetse fly species trapped in the study area with apparent density of 20.6. The overall prevalence of Trypanosoma spp. in cattle and goats was 38.9% and 37% respectively for samples collected in 2015 while the prevalence of Trypanosome spp in cattle samples collected in 2017 was 38%. In 2015, T. brucei was the highest prevalent trypanosome in both cattle (23%) and goats (18.8%). In both cattle and goats, a mixed infection of T. brucei + T. congolense was most encountered with prevalence of 4.8% and 4.1% in cattle and goats, respectively. In goats a mixed infection of T. brucei + T. congolense + T. vivax was higher (2.8%) than in cattle (2.4%). In 2017, in cattle (n = 250), the prevalence for T. congolense was 32.4%, T. vivax was 6.8% and T. brucei was 6.4%. A co-infection of T. brucei and T. congolense was most prevalent (7.4%). Only 3.2% of the cattle were co-infected with all the three Trypanosome species.
Conclusions

Current findings show that there are two types of Tsetse fly specie, s important in transmission of AAT. Presence of these parasites in goats shows that they also play a key role in epidemiology of the disease and control efforts should aim also involve goat farmers.
Smartphone application for celiac patients: assessing its effect on gastrointestinal symptoms in a randomized controlled clinical trial
Authors: Nikniaz Zeinab , Shirmohammadi Masood , Masserat Elham , Namvar Zahra Akbari
Published: June 2020

Background: We aimed to study the effectiveness of a “celiac” smartphone application on gastrointestinal symptoms in celiac patients. Methods: In the present randomized controlled clinical trial, 60 celiac patients were randomly assigned to receive education through a smartphone application (n=30) or conventional clinical education (n=30). We assessed the gastrointestinal symptoms using the gastrointestinal symptom rating scale (GSRS) questionnaire at baseline and three months after interventions. The GSRS total score, celiac disease GSRS (CD-GSRS) score, abdominal pain, reflux, diarrhea, constipation, and indigestion scores were calculated. Results: Out of 60 randomized patients, 58 patients completed the study. In comparison to baseline, the mean score of GSRS total score (p=0.04), CD-GSRS score (p=0.001), and indigestion subscore (p<0.001) were significantly decreased in the intervention group. In the control group, compared to baseline, the mean scores of CD-GSRS score (p=0.03) and abdominal pain subscore (p=0.04) were significantly reduced; and the mean score of reflux (p=0.01) subscore was significantly increased. The results of the between-group comparisons showed that although the mean GSRS and CD-GSRS total scores were lower in the intervention group compared with the control group, the differences were not statistically significant. In terms of GSRS subscores, there was a significant difference between the two groups only in the mean score of indigestion (P=0.01).Conclusion: According to the results, smartphone application had a significantly positive effect on indigestion symptoms compared with routine clinic education. However, more large studies with longer duration of the follow-up period are needed to assure this approach Trial registration: The protocol of the study was registered in the Iranian registry of clinical trials (IRCT code: IRCT20170117032004N2). trial registry webpage: : https://www.irct.ir/user/trial/40187/view. Trial registry date: 2019.6.26
Intrinsic laws of k-mer spectra of genome sequences and evolution mechanism of genomes
Authors: Zhenhua Yang , Li Hong , Yun Jia , Yan Zheng , Hu Meng , Tonglaga Bao , Xiaolong Li , Liaofu Luo
Published: June 2020

Background K-mer spectra of DNA sequences contain important information about sequence composition and sequence evolution. We want to reveal the evolution rules of genome sequences by studying the k-mer spectra of genome sequences. Results The intrinsic laws of k-mer spectra of 920 genome sequences from primate to prokaryote were analyzed. We found that there are two types of evolution selection modes in genome sequences, named as CG Independent Selection and TA Independent Selection. There is a mutual inhibition relationship between CG and TA independent selections. We found that the intensity of CG and TA independent selections correlates closely with genome evolution and G+C content of genome sequences. The living habits of species are related closely to the independent selection modes adopted by species genomes. Consequently, we proposed an evolution mechanism of genomes in which the genome evolution is determined by the intensities of the CG and TA independent selections and the mutual inhibition relationship. Besides, by the evolution mechanism of genomes, we speculated the evolution modes of prokaryotes in mild and extreme environments in the anaerobic age and the evolving process of prokaryotes from anaerobic to aerobic environment on earth as well as the originations of different eukaryotes. Conclusion We found that there are two independent selection modes in genome sequences. The evolution of genome sequence is determined by the two independent selection modes and the mutual inhibition relationship between them.
Risk factors and electrocardiogram characteristics for mortality of critical inpatients with COVID-19
Authors: Li Lingzhi , Zhang Shudi , He Bing , Chen Xiaobei , Wang Shihong , Qingyan Zhao
Published: June 2020

Background
The novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has subsequently spread worldwide. The number of death has increased rapidly. However, the possible risk factors that lead to death in critical inpatients with coronavirus disease 2019 (COVID-19) are not yet fully known. This study was to explore the risk factors for mortality of critical inpatients with COVID-19.
Methods
In this single-centered, retrospective study, we enrolled 113 critical patients with COVID-19 in Renmin Hospital of Wuhan University between Feb 1, 2020 and Mar 15, 2020. Data were collected using a standard method including clinical records and laboratory findings. Outcomes of survivors and death were compared.
Results
A total of 113 critical patients (from 29 to 95 years) with COVID-19 were recruited, 50 (44.25%) died and 63 recovered (55.75%). The proportion of patients with ventricular arrhythmia was higher in the death group than the recovery group (24.0% vs 4.4%; p = 0.021), and was higher among myocardial damage cases than non-myocardial damage cases (26.1% vs 4.3%; p = 0.013). Multivariate analysis confirmed four independent predictors related to mortality of COVID-19: age > 70 yrs (HR 1.84, 95% CI 1.03–3.28), initial neutrophil count more than 6.5 × 109/L (HR 3.43, 95% CI 1.84–6.40), C-reactive protein greater more than 100 mg/L (HR 1.93, 95% CI 1.04–3.59), and lactate dehydrogenase more than 300 U/L (HR 2.90, 95% CI 1.26–6.67). Immunoglobulin treatment (HR 0.39, 95% CI 0.21–0.73) can reduce the risk of death. There was no significant difference in the QT interval between patients with and without hydroxychloroquine treatment.
Conclusions
Old age (> 70 years), neutrophilia, C-reactive protein greater more than 100 mg/L and lactate dehydrogenase more than 300 U/L are high-risk factors for mortality of critical patients with COVID-19. The incidence of ventricular arrhythmia was higher in deceased patients than survivors.
Fertility Preserving Surgery in A Woman with Synchronous Endometrial and Ovarian Cancer Followed by Successful Pregnancy Outcome: Case Report and Review of Literature
Authors: Suri Vanita , Bansal Ramandeep , Aggarwal Neelam , Sikka Pooja , Chopra Seema , Saha Subhash Chandra , Gupta Nalini , Rai Bhavana
Published: June 2020

Background: Successful pregnancy outcome in women with synchronous ovarian and endometrial cancers is very rare. We report successful pregnancy outcome in a young woman managed conservatively for synchronous endometrial and ovarian cancer. Case presentation: 30 years old nulliparous lady presented following exploratory laparotomy, left salpingo-oophorectomy and hysteroscopic polypectomy for left adnexal mass. Histology revealed endometrioid carcinoma of left ovary and moderately differentiated adenocarcinoma in the resected polyp. She underwent staging laparotomy along with hysteroscopy which confirmed above findings without any evidence of further tumor spread. She was treated conservatively with high dose oral progestin (megestrol acetate, 160mg) and leuprolide acetate 3.75mg monthly injections for three months along with four cycles of carboplatin and paclitaxel based chemotherapy followed by monthly injection of leuprolide for further three months. After failure of spontaneous conception, she underwent ovulation induction for six cycles along with intrauterine insemination which failed. She underwent in vitro fertilization with donor egg followed by elective cesarean section at 37weeks of gestation. She delivered a healthy baby of weight 2.7 Kg. Intraoperatively 5×6cm right ovarian cyst was found which drained chocolate coloured fluid on puncture and cystectomy was carried out. Histological examination revealed endometrioid cyst of right ovary. Uterus was spared as she wanted to preserve her fertility. She is being followed periodically and is normal nine months following delivery. She is on injection Depot medroxy progesterone acetate once every three months.
Correlation between facet tropism and lumbar disc herniation
Authors: Ye Han , Xiaodong Wang , Zepei Zhang , Shrestha Deepak , Kepeng Li , Yang Song , Jun Miao
Published: June 2020

Purpose The relationship between facet tropism (FT) and lumbar disc herniation (LDH) is not clear. In the present study, MRI was used to assess the relationship between lumbar facet joint tropism and disc herniation. This paper is the first to assess the relationship between facet joint tropism and LDH from the sagittal view. Methods 122 patients (154 segments) with lumbar disc herniation and 102 normal subjects (306 segments) who visited our hospital from 01-Jan-2020 to 01-Apr-2020 were included in this study. The axial and sagittal angles of facet joints were measured using an MRI scan. The difference of 10° in facet angles between left and right was defined as lumbar facet tropism. The relationship between the two was analyzed. Results In the axial, 95/154 segments with lumbar disc herniation had FT whereas, 48/258 segments had FT in the normal segments(p < 0.01). In the sagittal, 61/154 segments with lumbar disc herniation had FT and 48/306 had FT in the control(p < 0.01). Conclusion The proportion of FT is better in patients with lumbar disc herniation, and there is a significant correlation between FT and lumbar disc herniation.
Adsorptive removal of malachite green dye from aqueous solution onto activated carbon of Catha edulis stem as a low cost bio-adsorbent
Authors: Abate Gietu yirga , Alene Adugna Nigatu , Habte Adere Tarekegne , Getahun Desiew Mekuanint
Published: June 2020

Background: The release of hazardous synthetic dyes into industrial effluents has emerged as an environmental problem requiring remediation. The present study focused on the preparation of a new and environmentally-friendly material (adsorbent) for the remediation of aqueous media containing dye. The low cost adsorbent was prepared from locally available khat (Catha edulis) stem which considered as waste and accumulated on waste disposal areas of woldia town, Ethiopia. Comprehensive characterization studies were carried out on the bio-adsorbent such as proximate analyses, specific surface area, and Fourier-Transform Infrared (FTIR) spectroscopy (which shows functional groups on adsorbents surface). Results: The proximate analysis shows the prepared adsorbent has very high fixed carbon content (83.65%), which refers to high quality of the adsorbent. The adsorption performance of the prepared activated carbon was optimize by varying operating parameters such as initial dye concentration (10 mg/L), pH (10), dosage (0.5 g), and contact time (60 min). The maximum removal efficiency of the prepared adsorbent at those optimum conditions was 98.8%. The experimental data was tested by most common kinetics and isotherm models. It was observed that the pseudo-second-order kinetic model fits better with good correlation coefficient and the equilibrium data fitted well with the Freundlich isotherm model. Conclusion: In summery this study demonstrated that the waste bio sorbent could be employed as an effective and eco-friendly alternative for the cleanup of dye-polluted aqueous system.
MtNIP5;1, A Novel Medicago Truncatula Boron Diffusion Facilitator Induced Under Deficiency
Authors: Granado-Rodríguez Sara , Bolaños Luis , Reguera Maria
Published: June 2020

Background: Boron (B) uptake in plants implies a passive diffusion through membranes under optimal conditions. Under deficiency or toxicity, the participation of two distinct protein families (the aquaporin family Major intrinsic proteins (MIPs) and the Boron transporter family BOR) is required to minimize detrimental effects caused by B stress that ends up inhibiting growth and altering development. Legumes comprise important crops that offer major agronomic benefits including the capacity of establishing symbiosis with rhizobia fixing atmospheric N2 and for being important food protein sources. It is well proven their susceptibility to B stress leading to important yield penalties. However, little is known about the transport mechanisms responsible for B uptake and distribution in legumes, especially under deficiency. Results: A novel legume B transporter involved in B uptake under deficiency, the Medicago truncatula homologous protein MtNIP5;1 (Medtr1g097840) to the Arabidopsis thaliana AtNIP5;1 (sharing 73,7% amino acid identity) was identified. Further analyses revealed that this M. truncatula aquaporin expression was boron-regulated in roots, being induced under deficiency and repressed under toxicity. It localizes at the plasma membrane of root epidermal cells and in nodules, where B plays pivotal roles in symbiosis. Furthermore, a partial complementation of the nip5;1 Arabidopsis mutant phenotype under B deficiency supports a functional role of MtNIP5;1 as a B transporter of in this legume model plant. Conclusions: The results here presented support a functional role of MtNIP5;1 uptaking B under deficiency providing new insights into this micronutrient transport mechanisms in legume species.