bluuebunny/crossref_metadata_2025 huggingface dataset:
Myeloid Cell Modulation by Tumor-Derived Extracellular Vesicles
Authors: Arkhypov Ihor , Lasser Samantha , Petrova Vera , Weber Rebekka , Groth Christopher , Utikal Jochen , Altevogt Peter , Umansky Viktor
Published: August 2020

Extracellular vesicles (EV) can carry proteins, RNA and DNA, thus serving as communication tools between cells. Tumor cells secrete EV, which can be taken up by surrounding cells in the tumor microenvironment as well as by cells in distant organs. Tumor-derived EV (TEV) contain factors induced by tumor-associated hypoxia such as heat shock proteins or a variety of microRNA (miRNA). The interaction of TEV with tumor and host cells can promote cancer angiogenesis, invasion and metastasis. Myeloid cells are widely presented in tissues, comprise the majority of immune cells and play an essential role in immune reactions and tissue remodeling. However, in cancer, the differentiation of myeloid cells and their functions are impaired, resulting in tumor promotion. Such alterations are due to chronic inflammatory conditions associated with cancer and are mediated by the tumor secretome, including TEV. A high capacity of myeloid cells to clear EV from circulation put them in the central position in EV-mediated formation of pre-metastatic niches. The exposure of myeloid cells to TEV could trigger numerous signaling pathways. Progenitors of myeloid cells alter their differentiation upon the contact with TEV, resulting in the generation of myeloid-derived suppressor cells (MDSC), inhibiting anti-tumor function of T and natural killer (NK) cells and promoting thereby tumor progression. Furthermore, TEV can augment MDSC immunosuppressive capacity. Different subsets of mature myeloid cells such as monocytes, macrophages, dendritic cells (DC) and granulocytes take up TEV and acquire a protumorigenic phenotype. However, the delivery of tumor antigens to DC by TEV was shown to enhance their immunostimulatory capacity. The present review will discuss a diverse and complex EV-mediated crosstalk between tumor and myeloid cells in the context of the tumor type, TEV-associated cargo molecules and type of recipient cells.
Emerging Roles of the Selective Autophagy in Plant Immunity and Stress Tolerance
Authors: Ran Jie , Hashimi Sayed M. , Liu Jian-Zhong
Published: August 2020

Autophagy is a conserved recycling system required for cellular homeostasis. Identifications of diverse selective receptors/adaptors that recruit appropriate autophagic cargoes have revealed critical roles of selective autophagy in different biological processes in plants. In this review, we summarize the emerging roles of selective autophagy in both biotic and abiotic stress tolerance and highlight the new features of selective receptors/adaptors and their interactions with both the cargoes and Autophagy-related gene 8s (ATG8s). In addition, we review how the two major degradation systems, namely the ubiquitin–proteasome system (UPS) and selective autophagy, are coordinated to cope with stress in plants. We especially emphasize how plants develop the selective autophagy as a weapon to fight against pathogens and how adapted pathogens have evolved the strategies to counter and/or subvert the immunity mediated by selective autophagy.
The Lysine Specific Demethylase-1 Negatively Regulates the COL9A1 Gene in Human Articular Chondrocytes
Authors: Durand Anne-Laure , Dufour Alexandre , Aubert-Foucher Elisabeth , Oger-Desfeux Christine , Pasdeloup Marielle , Lustig Sebastien , Servien Elvire , Vaz Gualter , Perrier-Groult Emeline , Mallein-Gerin Frederic , Lafont Jerome E.
Published: August 2020

Osteoarthritis (OA) is a degenerative disease of the joints which is associated with an impaired production of the cartilage matrix by the chondrocytes. Here, we investigated the role of Lysine-Specific Demethylase-1 (LSD1), a chromatin remodeling enzyme whose role in articular chondrocytes was previously associated with a catabolic activity and which is potentially involved during OA. Following a loss of function strategy and RNA sequencing analysis, we detail the genes which are targeted by LSD1 in human articular chondrocytes and identify COL9A1, a gene encoding the α1 chain of the cartilage-specific type IX collagen, as negatively regulated by LSD1. We show that LSD1 interacts with the transcription factor SOX9 and is recruited to the promoter of COL9A1. Interestingly, we observe that OA cartilage displays stronger LSD1 immunostaining compared with normal, and we demonstrate that the depletion of LSD1 in OA chondrocytes prevents the decrease in COL9A1 following Il-1β treatment. These results suggest LSD1 is a new regulator of the anabolic activity of articular chondrocytes potentially destabilizing the cartilage matrix, since it negatively regulates COL9A1, a gene encoding a crucial anchoring collagen molecule. This newly identified role played by LSD1 may thus participate in the alteration of the cartilage matrix during OA.
Analysis of Membrane Transport Equations for Reverse Electrodialysis (RED) Using Irreversible Thermodynamics
Authors: Kujawski Wojciech , Yaroshchuk Andriy , Zholkovskiy Emiliy , Koter Izabela , Koter Stanislaw
Published: August 2020

Reverse electrodialysis (RED) is an electro-membrane process for the conversion of mixing energy into electricity. One important problem researchers’ face when modeling the RED process is the choice of the proper membrane transport equations. In this study, using experimental data that describe the membrane Nafion 120 in contact with NaCl aqueous solutions, the linear transport equation of irreversible thermodynamics was applied to calculate the power density of the RED system. Various simplifying assumptions about transport equation (i.e., four-, three-, and two-coefficients approaches) are proposed and discussed. We found that the two-coefficients approach, using the membrane conductivity and the apparent transport number of ions, describes the power density with good accuracy. In addition, the influence of the membrane thickness and the concentration polarization on the power density is also demonstrated.
Antibiotic-Resistant and Non-Resistant Bacteria Display Similar Susceptibility to Dielectric Barrier Discharge Plasma
Authors: Sakudo Akikazu , Misawa Tatsuya
Published: August 2020

Here, we examined whether antibiotic-resistant and non-resistant bacteria show a differential susceptibility to plasma treatment. Escherichia coli DH5α were transformed with pPRO-EX-HT-CAT, which encodes an ampicillin resistance gene and chloramphenicol acetyltransferase (CAT) gene, and then treated with a dielectric barrier discharge (DBD) plasma torch. Plasma treatment reduced the viable cell count of E. coli after transformation/selection and further cultured in ampicillin-containing and ampicillin-free medium. However, there was no significant difference in viable cell count between the transformed and untransformed E. coli after 1 min- and 2 min-plasma treatment. Furthermore, the enzyme-linked immunosorbent assay (ELISA) and acetyltransferase activity assay showed that the CAT activity was reduced after plasma treatment in both transformed and selected E. coli grown in ampicillin-containing or ampicillin-free medium. Loss of lipopolysaccharide and DNA damage caused by plasma treatment were confirmed by a Limulus test and polymerase chain reaction, respectively. Taken together, these findings suggest the plasma acts to degrade components of the bacteria and is therefore unlikely to display a differential affect against antibiotic-resistant and non-resistant bacteria. Therefore, the plasma method may be useful in eliminating bacteria that are recalcitrant to conventional antibiotic therapy.
Identification of CD34+/PGDFRα+ Valve Interstitial Cells (VICs) in Human Aortic Valves: Association of Their Abundance, Morphology and Spatial Organization with Early Calcific Remodeling
Authors: Lis Grzegorz J. , Dubrowski Andrzej , Lis Maciej , Solewski Bernard , Witkowska Karolina , Aleksandrovych Veronika , Jasek-Gajda Ewa , Hołda Mateusz K. , Gil Krzysztof , Litwin Jan A.
Published: August 2020

Aortic valve interstitial cells (VICs) constitute a heterogeneous population involved in the maintenance of unique valvular architecture, ensuring proper hemodynamic function but also engaged in valve degeneration. Recently, cells similar to telocytes/interstitial Cajal-like cells described in various organs were found in heart valves. The aim of this study was to examine the density, distribution, and spatial organization of a VIC subset co-expressing CD34 and PDGFRα in normal aortic valves and to investigate if these cells are associated with the occurrence of early signs of valve calcific remodeling. We examined 28 human aortic valves obtained upon autopsy. General valve morphology and the early signs of degeneration were assessed histochemically. The studied VICs were identified by immunofluorescence (CD34, PDGFRα, vimentin), and their number in standardized parts and layers of the valves was evaluated. In order to show the complex three-dimensional structure of CD34+/PDGFRα+ VICs, whole-mount specimens were imaged by confocal microscopy, and subsequently rendered using the Imaris (Bitplane AG, Zürich, Switzerland) software. CD34+/PDGFRα+ VICs were found in all examined valves, showing significant differences in the number, distribution within valve tissue, spatial organization, and morphology (spherical/oval without projections; numerous short projections; long, branching, occasionally moniliform projections). Such a complex morphology was associated with the younger age of the subjects, and these VICs were more frequent in the spongiosa layer of the valve. Both the number and percentage of CD34+/PDGFRα+ VICs were inversely correlated with the age of the subjects. Valves with histochemical signs of early calcification contained a lower number of CD34+/PDGFRα+ cells. They were less numerous in proximal parts of the cusps, i.e., areas prone to calcification. The results suggest that normal aortic valves contain a subpopulation of CD34+/PDGFRα+ VICs, which might be involved in the maintenance of local microenvironment resisting to pathologic remodeling. Their reduced number in older age could limit the self-regenerative properties of the valve stroma.
Effects of Early Life Stress on Epigenetic Changes of the Glucocorticoid Receptor 17 Promoter during Adulthood
Authors: Seo Mi Kyoung , Kim Seon-gu , Seog Dae-Hyun , Bahk Won-Myong , Kim Seong-Ho , Park Sung Woo , Lee Jung Goo
Published: August 2020

Growing evidence suggests that early life stress (ELS) has long-lasting effects on glucocorticoid receptor (GR) expression and behavior via epigenetic changes of the GR exon 17 promoter. However, it remains unclear whether ELS regulates histone modifications of the GR exon 17 promoter across the life span. We investigated the effects of maternal separation (MS) on histone acetylation and methylation of GR exon 17 promoter in the hippocampus, according to the age of adults. Depression-like behavior and epigenetic regulation of GR expression were examined at young and middle adulthood in mice subjected to MS from postnatal day 1 to 21. In the forced swimming test, young adult MS mice showed no effect on immobility time, but middle-aged MS mice significantly increased immobility time. Young adult and middle-aged MS mice showed decreased GR expression. Their two ages showed decreased histone acetylation with increased histone deacetylases (HDAC5) levels, decreased permissive methylation, and increased repressive methylation at the GR exon 17 promoter. The extent of changes in gene expression and histone modification in middle adulthood was greater than in young adulthood. These results indicate that MS in early life causes long-term negative effects on behavior via histone modification of the GR gene across the life span.
Information Application of the Regional Development: Strategic Couplings in Global Production Networks in Jiangsu, China
Authors: Xu Haiying , Hsu Wei-Ling , Lee Yee-Chaur , Chern Tian-Yow , Luo Shr-Wei
Published: August 2020

The recent literature concerning globalizing regional development has placed significant emphasis on the Global Production Network (GPN 2.0). GPN 2.0 in economic geography emphasizes that regional growth is caused by a shift in the strategic coupling mode from a low to high level. In addition, GPN 2.0 regards firm-level value capture trajectories as key analytical object, rather than the interactive relationships among scalar and divergent actors in GPN 1.0. To provide a better understanding of causal linkages between the GPNs and uneven regional development in the background of globalization and to test the applicability of GPN 2.0 analysis framework, the paper analyzed 62 Korean-invested automotive firms in Jiangsu Province, China. In order to explore the value capture trajectories of lead firms in the GPNs, the authors applied K-means clustering method to quantitatively analyze the local supply networks of lead firms from organizational and spatial dimensions. Then, comparisons were made between strategic coupling modes of GPNs and regional development in North and South Jiangsu. This study found obvious similarities within these two regions but obvious differences between them in terms of value capture trajectories. We observed that North Jiangsu is currently in the stage of “structural coupling”, whereas South Jiangsu is in the stage of “functional coupling.” Thus, this article argues that spatial settings such as regional assets and autonomy are key factors influencing uneven economic development. This research may provide a crucial reference for the regional development of Jiangsu, China.
Impact of Azithromycin and/or Hydroxychloroquine on Hospital Mortality in COVID-19
Authors: Albani Filippo , Fusina Federica , Giovannini Alessia , Ferretti Pierluigi , Granato Anna , Prezioso Chiara , Divizia Danilo , Sabaini Alessandra , Marri Marco , Malpetti Elena , Natalini Giuseppe
Published: August 2020

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has led to widespread use of hydroxychloroquine and azithromycin despite the lack of conclusive evidence for their safety and efficacy. We evaluated the association between treatment with hydroxychloroquine and/or azithromycin and hospital mortality as the primary outcome. We compared the hospital mortality of patients treated with hydroxychloroquine alone, azithromycin alone, or their combination to the mortality of patients who received neither drug. A logistic multivariate model with overlap weight propensity score was used for estimation of odds ratios (ORs) with 95% confidence intervals (95% CIs). One thousand four hundred and three patients with SARS-CoV-2 infection were admitted to the hospital. At the time of the analysis, the outcome was available for 1376 (98%) of them. Five hundred and eighty-seven patients (42%) received azithromycin and 377 patients (27%) received hydroxychloroquine, alone or in combination. In-hospital mortality was 26%. After the adjusted analysis, azithromycin alone was associated with lower mortality (OR 0.60, 95% CI 0.42–0.85) compared to no treatment. Hydroxychloroquine alone (OR 0.76, 95% CI 0.53–1.08) and the combination of azithromycin and hydroxychloroquine (OR 1.13, 95% CI 0.77–1.69) were not associated with hospital mortality. In this cohort of patients, azithromycin alone was associated with lower hospital mortality but hydroxychloroquine was not associated with increased or reduced mortality. While we await randomized clinical trials, these data support the use of azithromycin in novel coronavirus disease 2019 (COVID-19) and can contribute to better understanding of its role in further meta-analyses.
Prevention of Type 1 Diabetes: Past Experiences and Future Opportunities
Authors: Beik Przemysław , Ciesielska Martyna , Kucza Maria , Kurczewska Alicja , Kuźmińska Joanna , Maćkowiak Bartosz , Niechciał Elżbieta
Published: August 2020

Type 1 diabetes (T1D) results from autoimmune destruction of insulin-producing beta-cells in the pancreas, caused by the interplay of genetic and environmental factors. Despite the introduction of advanced technologies for diabetes management, most patients fail to achieve target glycemic control, and T1D still has a high burden of long-term end-organ complications. Over several decades, multiple clinical trials have attempted to find prevention for T1D in at-risk individuals or to stabilize, ultimately reverse, the disease in those with T1D. To date, T1D remains yet incurable condition; however, recently improved understanding of the natural history of the disease may lead to new strategies to preserve or improve beta-cell function in those at increased risk and T1D patients. This publication aims to provide an overview of past experiences and recent findings in the prevention of T1D.
Craniocervical and Cervical Spine Features of Patients with Temporomandibular Disorders: A Systematic Review and Meta-Analysis of Observational Studies
Authors: Cuenca-Martínez Ferran , Herranz-Gómez Aida , Madroñero-Miguel Beatriz , Reina-Varona Álvaro , La Touche Roy , Angulo-Díaz-Parreño Santiago , Pardo-Montero Joaquín , del Corral Tamara , López-de-Uralde-Villanueva Ibai
Published: August 2020

To assess neck disability with respect to jaw disability, craniocervical position, cervical alignment, and sensorimotor impairments in patients with temporomandibular disorders (TMD), a systematic review and meta-analysis of observational studies trials were conducted. The meta-analysis showed statistically significant differences in the association between neck disability and jaw disability (standardized mean difference (SMD), 0.72 (0.56–0.82)). However, results showed no significant differences for cervical alignment (SMD, 0.02 (−0.31–0.36)) or for the craniocervical position (SMD, −0.09 (−0.27–0.09)). There was moderate evidence for lower pressure pain thresholds (PPT) and for limited cervical range of motion (ROM). There was limited evidence for equal values for maximal strength between the patients with TMD and controls. There was also limited evidence for reduced cervical endurance and conflicting evidence for abnormal electromyographic (EMG) activity and motor control in TMD patients. Results showed a clinically relevant association between cervical and mandibular disability in patients with TMD. Regarding sensory-motor alterations, the most conclusive findings were observed in the reduction of PPT and cervical ROM, with moderate evidence of their presence in the patients with TMD. Lastly, the evidence on impaired motor control and cervical EMG activity in patients with TMD was conflicting.
Early Biomarkers of Neurodegenerative and Neurovascular Disorders in Diabetes
Authors: Gasecka Aleksandra , Siwik Dominika , Gajewska Magdalena , Jaguszewski Miłosz J. , Mazurek Tomasz , Filipiak Krzysztof J. , Postuła Marek , Eyileten Ceren
Published: August 2020

Diabetes mellitus (DM) is a common disease worldwide. There is a strong association between DM and neurovascular and neurodegenerative disorders. The first group mainly consists of diabetic retinopathy, diabetic neuropathy and stroke, whereas, the second group includes Alzheimer’s disease, Parkinson’s disease, mild cognitive impairment and dementia. The aforementioned diseases have a common pathophysiological background including insulin resistance, oxidative stress, atherosclerosis and vascular injury. The increasing prevalence of neurovascular and neurodegenerative disorders among diabetic patients has resulted in an urgent need to develop biomarkers for their prediction and/or early detection. The aim of this review is to present the potential application of the most promising biomarkers of diabetes-related neurodegenerative and neurovascular disorders, including amylin, β-amyloid, C-reactive protein (CRP), dopamine, gamma-glutamyl transferase (GGT), glycogen synthase kinase 3β, homocysteine, microRNAs (mi-RNAs), paraoxonase 1, phosphoinositide 3-kinases, tau protein and various growth factors. The most clinically promising biomarkers of neurovascular and neurodegenerative complications in DM are hsCRP, GGT, homocysteine and miRNAs. However, all biomarkers discussed in this review could become a part of the potential multi-biomarker screening panel for diabetic patients at risk of neurovascular and neurodegenerative complications.
Evaluation of the Accero Stent for Stent-Assisted Coiling of Unruptured Wide-Necked Intracranial Aneurysm Treatment with Short-Term Follow-Up
Authors: Poncyljusz Wojciech , Kubiak Kinga , Sagan Leszek , Limanówka Bartosz , Kołaczyk Katarzyna
Published: August 2020

Background: Stent-assisted coiling is an effective method of treating intracranial aneurysms. The aim of the study was to assess the safety and efficacy of the new Accero stent for the treatment of intracranial aneurysms. Materials and Methods: It was a retrospective, single-center study. Eighteen unruptured intracranial aneurysms were treated using the stent-assisted coiling method with the Accero stent. Patient demographics, aneurysm characteristics, procedural parameters, grade of occlusion, complications, and clinical results were analyzed. Follow-up magnetic resonance (MR) was performed 6 months after intervention. Results: Seventeen patients with 18 incidental unruptured aneurysms were electively treated with coiling and the Accero stent. The aneurysms were located on internal carotid artery (ICA), middle cerebral artery (MCA) and basilar artery (BA). All stents were deployed successfully. Immediate complete occlusion rate Raymond-Roy occlusion classification (RROC) class I was achieved in 13 cases and class II in 4 cases. Complications occurred in 2/17 treatments and included guidewire stent perforation with subarachnoid hemorrhage (SAH) and stent deformation. Vascular spasm in the subarachnoid hemorrhage (SAH) patient subsided before discharge. Ninety days after intervention, the modified Rankin Scale (mRS) value was 0. RROC class I was observed in 88.23% of cases in follow-up. Conclusion: The Accero stent provides excellent support for coil mass. It constitutes an efficacious device with good initial occlusion rate for treating wide-necked unruptured intracranial aneurysms.
Individuals with Peripheral Artery Disease (PAD) and Type 1 Diabetes Are More Likely to Undergo Limb Amputation than Those with PAD and Type 2 Diabetes
Authors: Jain Nidhi , Agarwal Manyoo A. , Jalal Diana , Dokun Ayotunde O.
Published: August 2020

Background: Limited data exist comparing how type 1 diabetes mellitus (DM) and type 2 DM may have differential effects on peripheral artery disease (PAD) severity. We aimed to study the association of type of DM with the procedure utilized in hospitalizations with a diagnosis of PAD. Methods: We used the national inpatient sample databases from 2003 to 2014 to identify hospitalizations with a diagnosis of PAD and type 1 or type 2 DM. Logistic regression was utilized to evaluate the association between type of DM and procedure utilized (amputation-overall, major, endovascular revascularization, surgical revascularization). Results: We identified 14,012,860 hospitalizations with PAD diagnosis and DM, 5.6% (n = 784,720) had type 1 DM. The patients with type 1 DM were more likely to present with chronic limb-threatening ischemia (CLTI) (45.2% vs. 32.0%), ulcer (25.9% vs. 17.7%), or complicated ulcer (16.6% vs. 10.5%) (all p < 0.001) when compared to those with type 2 DM. Type 1 DM was independently and significantly associated with more amputation procedures (adjusted odds ratio = 1.12, 95% confidence interval [CI] I 1.08 to 1.16, p < 0.001). Overall, in-hospital mortality did not differ between the individuals with type 1 and type 2 DM. The overall mean (95% CI) length of stay (in days) was 6.6 (6.5 to 6.6) and was significantly higher for type 1 DM (7.8 [7.7 to 8.0]) when compared to those with type 2 DM (6.5 [6.4 to 6.6]). Conclusion: We observed that individuals with PAD and type 1 DM were more likely to present with CLTI and ulcer and undergo amputation when compared to those with PAD and type 2 diabetes. Further studies are needed to better understand the underlying mechanisms behind these findings and to identify novel interventions to reduce the risk of amputation in patients with type 1 DM.
Photo and Plasma Activation of Dental Implant Titanium Surfaces. A Systematic Review with Meta-Analysis of Pre-Clinical Studies
Authors: Pesce Paolo , Menini Maria , Santori Gregorio , Giovanni Emanuele De , Bagnasco Francesco , Canullo Luigi
Published: August 2020

Background: Ultraviolet (UV) and non-thermal plasma functionalization are surface treatment modalities that seem able to improve osseointegration. The aim of this systematic review and meta-analysis is to assess the effect of the two methods and possible differences. Materials and Methods: The systematic research of pre-clinical animal studies was conducted up to May 2020 in the databases PubMed/Medline, Scopus and the Cochrane Lybrary. A meta-analysis was performed by using the DerSimonian–Laird estimator in random-effects models. Results: Through the digital search, 518 articles were identified; after duplicate removal and screening process 10 papers were included. Four studies evaluating UV treatment in rabbits were included in the meta-analysis. The qualitative evaluation of the included studies showed that both UV photofunctionalization and non-thermal plasma argon functionalization of titanium implant surfaces might be effective in vivo to improve the osseointegration. The meta-analysis on four studies evaluating UV treatment in rabbits showed that bone to implant contact values (expressed as standardized mean differences and raw mean differences) were significantly increased in the bio-activated groups when follow-up times were relatively homogeneous, although a high heterogeneity (I2 > 75%) was found in all models. Conclusions: The present systematic review and meta-analysis on pre-clinical studies demonstrated that chair-side treatment of implants with UV or non-thermal plasma appear to be effective for improving osseointegration. This systematic review supports further clinical trials on this topic.
Quality Characteristics and Clinical Relevance of In-House 3D-Printed Customized Polyetheretherketone (PEEK) Implants for Craniofacial Reconstruction
Authors: Sharma Neha , Aghlmandi Soheila , Cao Shuaishuai , Kunz Christoph , Honigmann Philipp , Thieringer Florian M.
Published: August 2020

Additive manufacturing (AM) of patient-specific implants (PSIs) is gradually moving towards in-house or point-of-care (POC) manufacturing. Polyetheretherketone (PEEK) has been used in cranioplasty cases as a reliable alternative to other alloplastic materials. As only a few fused filament fabrication (FFF) printers are suitable for in-house manufacturing, the quality characteristics of the implants fabricated by FFF technology are still under investigated. This paper aimed to investigate PEEK PSIs fabricated in-house for craniofacial reconstruction, discussing the key challenges during the FFF printing process. Two exemplary cases of class III (Group 1) and class IV (Group 2) craniofacial defects were selected for the fabrication of PEEK PSIs. Taguchi’s L9 orthogonal array was selected for the following nonthermal printing process parameters, i.e., layer thickness, infill rate, number of shells, and infill pattern, and an assessment of the dimensional accuracy of the fabricated implants was made. The root mean square (RMS) values revealed higher deviations in Group 1 PSIs (0.790 mm) compared to Group 2 PSIs (0.241 mm). Horizontal lines, or the characteristic FFF stair-stepping effect, were more perceptible across the surface of Group 1 PSIs. Although Group 2 PSIs revealed no discoloration, Group 1 PSIs displayed different zones of crystallinity. These results suggest that the dimensional accuracy of PSIs were within the clinically acceptable range; however, attention must be paid towards a requirement of optimum thermal management during the printing process to fabricate implants of uniform crystallinity.
Understanding the Complexity of Sjögren’s Syndrome: Remarkable Progress in Elucidating NF-κB Mechanisms
Authors: Sisto Margherita , Ribatti Domenico , Lisi Sabrina
Published: August 2020

Sjögren’s syndrome (SS) is a systemic autoimmune inflammatory disease with a poorly defined aetiology, which targets exocrine glands (particularly salivary and lachrymal glands), affecting the secretory function. Patients suffering from SS exhibit persistent xerostomia and keratoconjunctivitis sicca. It is now widely acknowledged that a chronic grade of inflammation plays a central role in the initiation, progression, and development of SS. Consistent with its key role in organizing inflammatory responses, numerous recent studies have shown involvement of the transcription factor nuclear factor κ (kappa)-light-chain-enhancer of activated B cells (NF-κB) in the development of this disease. Therefore, chronic inflammation is considered as a critical factor in the disease aetiology, offering hope for the development of new drugs for treatment. The purpose of this review is to describe the current knowledge about the NF-κB-mediated molecular events implicated in the pathogenesis of SS.
Prevalence of Hangover Resistance According to Two Methods for Calculating Estimated Blood Alcohol Concentration (eBAC)
Authors: Terpstra Chantal , Scholey Andrew , Verster Joris C. , Benson Sarah
Published: August 2020

Hangover resistance may be linked to an increased risk of continuing harmful drinking behaviours as well as involvement in potentially dangerous daily activities such as driving while hungover, mainly due to the absence of negative consequences (i.e., hangover symptoms) the day after alcohol consumption. The aim of this study was to examine the occurrence of claimed alcohol hangover resistance relative to estimated blood alcohol concentration (eBAC). A total of 1198 participants completed an online survey by answering questions regarding their demographics, alcohol consumption and occurrence of hangover. Two methods were used to calculate eBAC, one based on the modified Widmark Equation (N = 955) and the other from an equation averaging the total body water (TBW) estimates of Forrest, Watson, Seidl, Widmark and Ulrich (males only) (N = 942). The percentage of participants who claimed to be hangover resistant decreased rapidly with increasing eBAC and only a small number of hangover resistant drinkers remained at higher eBACs. Comparisons of the eBACs calculated by the two methods revealed significantly higher BACs when using the modified Widmark equation. These findings suggest that additional research for eBAC calculations is needed to improve accuracy and comprehensiveness of these equations for future alcohol hangover research.
Voice Characteristics in Smith–Magenis Syndrome: An Acoustic Study of Laryngeal Biomechanics
Authors: Hidalgo-De la Guía Irene , Garayzábal-Heinze Elena , Gómez-Vilda Pedro
Published: August 2020

Smith–Magenis syndrome (SMS) is a rare genetic disease characterized by intellectual disability, serious behavior disorders, neurodevelopment delay, and speech and language disorders. An acoustic and biomechanical analysis of the voice of SMS young adults was carried out due to (a) the close relationship between the laryngeal biomechanics and the clinical and emotional state of a person; (b) the fact that no research on the voice in this syndrome has been conducted previously. The vocal timbre of most people diagnosed with SMS does not seem to be according to the complexion of diagnosed individuals, nor to their gender and age, so it could be interesting to attend the analysis of phonation of people with a rare genetic syndrome such as SMS. We used BioMetPhon, a specific piece of software to analyze the glottal source and biomechanics of vocals folds. Nineteen features related to dysphonia, physiology, and biomechanics of the vocal folds were considered. The adult phonation of 9 individuals with SMS was analyzed and compared to 100 normative male and female adult voices. Results showed that the phonation of the SMS group significantly deviates from the adult normophonic profile in more than one of the 19 features examined, such as stiffness of the thyroarytenoid muscle and dynamic mass of the vocal fold cover, among others.
Mitonuclear Interactions in the Maintenance of Mitochondrial Integrity
Authors: Karakaidos Panagiotis , Rampias Theodoros
Published: August 2020

In eukaryotic cells, mitochondria originated in an α-proteobacterial endosymbiont. Although these organelles harbor their own genome, the large majority of genes, originally encoded in the endosymbiont, were either lost or transferred to the nucleus. As a consequence, mitochondria have become semi-autonomous and most of their processes require the import of nuclear-encoded components to be functional. Therefore, the mitochondrial-specific translation has evolved to be coordinated by mitonuclear interactions to respond to the energetic demands of the cell, acquiring unique and mosaic features. However, mitochondrial-DNA-encoded genes are essential for the assembly of the respiratory chain complexes. Impaired mitochondrial function due to oxidative damage and mutations has been associated with numerous human pathologies, the aging process, and cancer. In this review, we highlight the unique features of mitochondrial protein synthesis and provide a comprehensive insight into the mitonuclear crosstalk and its co-evolution, as well as the vulnerabilities of the animal mitochondrial genome.
The Identification of an Adequate Stressing Level to Find the Proper Running-In Conditions of a Lubricated DLC-Metal-System
Authors: Faller Joachim , Scherge Matthias
Published: August 2020

Using a tribometer equipped with a high-resolution wear measurement unit (RNT), the running-in of a diamondlike carbon (DLC) iron spray coating contact was analyzed and optimized. The optimization comprised an initial parameter field with different load and speed levels to find key operation points. These points were used to compose a dedicated running-in parameter field. The analysis underlined the importance of identifying the adequate stressing conditions. With respect to our concept of the running-in corridor, a high-power running-in has to be preferred to obtain a tribological system with low friction, small total wear and wear rate, high system stability, and low sensitivity to external changes.
Effect of GelMA Hydrogel Coatings on Corrosion Resistance and Biocompatibility of MAO-Coated Mg Alloys
Authors: Weng Wenxian , Wu Weiwei , Yu Xiaoming , Sun Mingyue , Lin Zhensheng , Ibrahim Muhammad , Yang Huazhe
Published: August 2020

Micro-arc oxidation (MAO) treatment is a simple and effective technique to improve the corrosion resistance for magnesium alloys. However, the presence of micro-pores and cracks on the coatings provides paths for corrosive ions to penetrate into and react with the substrate, limiting the long-term corrosion resistance. In this paper, we designed a composite coating with which GelMA hydrogel coatings with varying thicknesses were prepared on the surface of MAO-coated magnesium alloys via a dip-coating method, aiming to improve the biocorrosion resistance and biocompatibility. The surface morphology, the chemical composition of GelMA hydrogels, and the crystallographic structure of magnesium alloys were characterized by scanning electron microscope (SEM), Fourier-transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD), respectively. The corrosion resistance and biocompatibility of all samples were evaluated through electrochemical and biological experiments. The results demonstrated that the addition of GelMA hydrogel could effectively seal the pores and improve the corrosion resistance and biocompatibility of MAO-coated magnesium alloys, especially for the sample with one layer of GelMA hydrogel, showing high cell proliferation rate, and its current density (Icorr) was two orders of magnitude lower than that of the MAO coating. Besides, the balance mechanism between corrosion and protection was proposed. As a result, the GelMA hydrogel coatings are beneficial to the application of MAO-coated magnesium alloys in bone tissue engineering and other fields.
Small Caliber Bulletproof Test of Warships’ Hulls
Authors: Kiciński Radosław , Kubit Andrzej
Published: August 2020

The article presents the characteristics of 1.3964 steel and the results of firing a 7.62 mm projectile with a steel core. A simplified Johnson–Cook material model for steel and projectile was used. Then, a FEM (finite element method) simulation was prepared to calibrate the material constants and boundary conditions necessary to be used in simulations of the entire hull model. It was checked how projectile modeling affects the FEM calculation results. After obtaining the simulation results consistent with the experimental results, using the model of a modern minehunter, the resistance of the ship’s hull to penetration by a small-caliber projectile was tested.
Improvement of Fatigue Life of GH3039 Superalloy by Laser Shock Peening
Authors: Tang Yang , Ge MaoZhong , Zhang Yongkang , Wang Taiming , Zhou Wen
Published: August 2020

In order to improve fatigue life of GH3039 superalloy, GH3039 superalloy sheets were treated by laser shock peening (LSP). The microstructure of GH3039 superalloy before and after LSP was characterized using an optical microscope, transmission electron microscope (TEM), and X-ray diffractometer. The fatigue life of the samples with and without LSP was investigated by fatigue experiments. Moreover, surface profile and residual stress were also examined. Experimental results indicated that the grains in the surface layer of the LSP sample were remarkably refined and reached the nanometer scale. The average surface roughness increased from 0.024 μm to 0.19 μm after LSP. The average fatigue life of the laser treated samples was 2.01 times larger than that of the untreated specimens. Additionally, mathematical statistical analysis confirms that LSP has a significant influence on the fatigue life of GH3039 superalloy. The improvement of fatigue life for the laser processed GH3039 superalloy was mainly attributed to compressive residual stress and grain refinement generated by LSP.
Weight-Vibration Pareto Optimization of a Triple Mass Flywheel for Heavy-Duty Truck Powertrains
Authors: Berbyuk Viktor
Published: August 2020

Enhanced efficiency of heavy-duty truck powertrains with constraints imposed on noise, vibration, and harshness requires novel solutions for torsion vibrations attenuation. In the paper, the weight-vibration Pareto optimization problem for a novel vibration absorber, a triple mass flywheel, for application in heavy-duty truck powertrains is considered. Global sensitivity analysis and Pareto optimization method are used to design a novel vibration absorber. The optimization method attempts to minimize oscillations of the torque at the transmission input shaft as well as to minimize total mass inertia of the absorber. It is shown that there exists a Pareto front between the measure of the attenuation of oscillations of the torque and the total mass inertia of a triple mass flywheel. The optimized design parameters for the absorber are obtained that provide the best attenuation of oscillations of the torque at the transmission input shaft for different mean values of the engine driving torque. The analysis shows real evidence of the feasibility of the application of this concept of vibration absorbers in heavy-duty truck powertrains. It is also shown that optimized design parameters of a triple mass flywheel put this concept in a superior position in comparison with a dual mass flywheel.
Zero-Hopf Bifurcations of 3D Quadratic Jerk System
Authors: Sang Bo , Huang Bo
Published: August 2020

This paper is devoted to local bifurcations of three-dimensional (3D) quadratic jerk system. First, we start by analysing the saddle-node bifurcation. Then we introduce the concept of canonical system. Next, we study the transcritial bifurcation of canonical system. Finally we study the zero-Hopf bifurcations of canonical system, which constitutes the core contributions of this paper. By averaging theory of first order, we prove that, at most, one limit cycle bifurcates from the zero-Hopf equilibrium. By averaging theory of second order, third order, and fourth order, we show that, at most, two limit cycles bifurcate from the equilibrium. Overall, this paper can help to increase our understanding of local behaviour in the jerk dynamical system with quadratic non-linearity.
Boundedness of a Class of Oscillatory Singular Integral Operators and Their Commutators with Rough Kernel on Weighted Central Morrey Spaces
Authors: Zhou Yongliang , Yan Dunyan , Wei Mingquan
Published: August 2020

In this paper, we establish the boundedness of a class of oscillatory singular integral operators with rough kernel on central Morrey spaces. Moreover, the boundedness for each of their commutators on weighted central Morrey spaces was also obtained. We generalized some existing results.
Semantic Segmentation for Aerial Mapping
Authors: Martinez-Soltero Gabriel , Alanis Alma Y. , Arana-Daniel Nancy , Lopez-Franco Carlos
Published: August 2020

Mobile robots commonly have to traverse rough terrains. One way to find the easiest traversable path is by determining the types of terrains in the environment. The result of this process can be used by the path planning algorithms to find the best traversable path. In this work, we present an approach for terrain classification from aerial images while using a Convolutional Neural Networks at the pixel level. The segmented images can be used in robot mapping and navigation tasks. The performance of two different Convolutional Neural Networks is analyzed in order to choose the best architecture.
An Improved Grey Wolf Optimizer for a Supplier Selection and Order Quantity Allocation Problem
Authors: Alejo-Reyes Avelina , Cuevas Erik , Rodríguez Alma , Mendoza Abraham , Olivares-Benitez Elias
Published: August 2020

Supplier selection and order quantity allocation have a strong influence on a company’s profitability and the total cost of finished products. From an optimization perspective, the processes of selecting the right suppliers and allocating orders are modeled through a cost function that considers different elements, such as the price of raw materials, ordering costs, and holding costs. Obtaining the optimal solution for these models represents a complex problem due to their discontinuity, non-linearity, and high multi-modality. Under such conditions, it is not possible to use classical optimization methods. On the other hand, metaheuristic schemes have been extensively employed as alternative optimization techniques to solve difficult problems. Among the metaheuristic computation algorithms, the Grey Wolf Optimization (GWO) algorithm corresponds to a relatively new technique based on the hunting behavior of wolves. Even though GWO allows obtaining satisfying results, its limited exploration reduces its performance significantly when it faces high multi-modal and discontinuous cost functions. In this paper, a modified version of the GWO scheme is introduced to solve the complex optimization problems of supplier selection and order quantity allocation. The improved GWO method called iGWO includes weighted factors and a displacement vector to promote the exploration of the search strategy, avoiding the use of unfeasible solutions. In order to evaluate its performance, the proposed algorithm has been tested on a number of instances of a difficult problem found in the literature. The results show that the proposed algorithm not only obtains the optimal cost solutions, but also maintains a better search strategy, finding feasible solutions in all instances.
Local and Global Dynamics of a Constraint Profit Maximization for Bischi–Naimzada Competition Duopoly Game
Authors: Askar Sameh S , Al-Khedhairi Abdulrahman
Published: August 2020

The Bischi–Naimzada game is a market competition between two firms with the objective of maximizing profits under limited information. In this article, we study a more generalized and realistic situation that takes into account the sales constraints. we generalize the economic model suggested by Bischi–Naimzada by introducing and studying the maximization of profits based on sales constraints. Our motivation in this paper is the studying of profit and sales constraints maximization and their influences on the game’s dynamics. The local stability of the equilibrium points of the proposed model is discussed. It examines how the dynamics of the proposed two-dimensional competition game model focusing on changes in both the speed of the adjustment and the sales constraint parameters. The map describing the game is proven to be noninvertible and yields many multi-stable, complex dynamics and the coexistence chaotic attractors may arise. The global behavior of the map is achieved by studying the critical curves. The numerical simulations demonstrate the coexistence of two attractors and complex structures of the attraction basins. Several examples are discussed in order to confirm all the analytical results obtained.
Incidence of Bladder Cancer in Type 2 Diabetes Mellitus Patients: A Population-Based Cohort Study
Authors: Pan Yueh , Lee Chia-Yi , Lee Liang-Ming , Wen Yu-Ching , Huang Jing-Yang , Yang Shun-Fa , Hsiao Chi-Hao
Published: August 2020

Background and objectives: Type 2 diabetes mellitus (T2DM) is becoming increasingly prevalent worldwide and is associated with increased incidence of kidney cancer and bladder cancer (BC). However, studies have produced conflicting results. Therefore, we retrospectively evaluated the incidence of BC in T2DM patients using the Taiwan National Health Insurance Research Database (NHIRD). Materials and Methods: We included 31,932 patients with a diagnosis of T2DM in the study group and 63,864 age- and sex-matched patients without T2DM at a ratio of 1:2 in the control group. The primary outcome was the diagnosis of BC. Cox proportional hazards regression was used to evaluate the incidence and adjusted hazard ratio (aHR) of BC in the multivariate model. Results: After a 16-year follow-up, we found that 67 BC cases occurred in the study group and 152 BC events in the non-T2DM group without a significantly higher risk (aHR: 0.842, 95% confidence interval: 0.627–1.13). Conclusions: T2DM patients do not have a higher risk of BC.
Epidemiologic Characteristics of Suicide in Panama, 2007–2016
Authors: Núñez-Samudio Virginia , Jiménez-Domínguez Aris , López Castillo Humberto , Landires Iván
Published: August 2020

Background and objectives: We aim to describe the demographic characteristics associated with suicide in Panama, to estimate the suicide mortality rate and years of potential life lost (YPLL) to suicide, and to explore the correlation of suicide rates with the Multidimensional Poverty Index (MPI). We present a descriptive retrospective epidemiological report of suicide-related mortality (Panama, 2007–2016). Materials and Methods: Data were matched-merged to calculate unadjusted suicide mortality rates (overall, and by sex, age groups, and administrative region), YPLL, and coefficients (r) for the correlation of MPI and suicide rates. Results: There were 1475 deaths by suicide (86% among men, 47% between 20 and 39 years). The average mortality rate was estimated at 3.91 per 100,000 population with an average YPLL rate of 3.79 per 1000 population. There was a statistically significant trend to reduce YPLL over time (r = −0.93; p< 0.001). Exploratory analyses did not show a significant correlation between the MPI and suicide rates. Our study showed a 6:1 male-to-female ratio of suicide, mostly affecting the age groups of 20–29 and over 80 years. Conclusions: Exploratory analyses on the correlation of the MPI and the suicide rates did not achieve statistical significance, and alternative explanations, such as access to pesticides and alcohol, were further explored to inform potential interventions.