bluuebunny/crossref_metadata_2025 huggingface dataset:
GWAS to Identify Novel QTNs for WSCs Accumulation in Wheat Peduncle Under Different Water Regimes
Authors: Gaur Arpit , Jindal Yogesh , Singh Vikram , Tiwari Ratan , Kumar Dinesh , Kaushik Deepak , Singh Jogendra , Narwal Sneh , Jaiswal Sarika , Iquebal Mir Asif , Angadi Ulavapp B. , Singh Gyanendra , Rai Anil , Singh Gyanendra Pratap , Sheoran Sonia
Published: March 2022

Water-soluble carbohydrates (WSCs) play a vital role in water stress avoidance and buffering wheat grain yield. However, the genetic architecture of stem WSCs’ accumulation is partially understood, and few candidate genes are known. This study utilizes the compressed mixed linear model-based genome wide association study (GWAS) and heuristic post GWAS analyses to identify causative quantitative trait nucleotides (QTNs) and candidate genes for stem WSCs’ content at 15 days after anthesis under different water regimes (irrigated, rainfed, and drought). Glucose, fructose, sucrose, fructans, total non-structural carbohydrates (the sum of individual sugars), total WSCs (anthrone based) quantified in the peduncle of 301 bread wheat genotypes under multiple environments (E01-E08) pertaining different water regimes, and 14,571 SNPs from “35K Axiom Wheat Breeders” Array were used for analysis. As a result, 570 significant nucleotide trait associations were identified on all chromosomes except for 4D, of which 163 were considered stable. A total of 112 quantitative trait nucleotide regions (QNRs) were identified of which 47 were presumable novel. QNRs qWSC-3B.2 and qWSC-7A.2 were identified as the hotspots. Post GWAS integration of multiple data resources prioritized 208 putative candidate genes delimited into 64 QNRs, which can be critical in understanding the genetic architecture of stem WSCs accumulation in wheat under optimum and water-stressed environments. At least 19 stable QTNs were found associated with 24 prioritized candidate genes. Clusters of fructans metabolic genes reported in the QNRs qWSC-4A.2 and qWSC-7A.2. These genes can be utilized to bring an optimum combination of various fructans metabolic genes to improve the accumulation and remobilization of stem WSCs and water stress tolerance. These results will further strengthen wheat breeding programs targeting sustainable wheat production under limited water conditions.
Integrated Metabolome and Transcriptome Analyses Reveal Dissimilarities in the Anthocyanin Synthesis Pathway Between Different Developmental Leaf Color Transitions in Hopea hainanensis (Dipterocarpaceae)
Authors: Huang Guihua , Liao Xuezhu , Han Qiang , Zhou Zaizhi , Liang Kunnan , Li Guangyou , Yang Guang , Tembrock Luke R. , Wang Xianbang , Wu Zhiqiang
Published: March 2022

Changes in plant leaf color during development are directly related to the accumulation or degradation of certain phytochemicals such as anthocyanins. Since some anthocyanins can be beneficial to human health and provide insights into the biology of leaves, the underlying processes and timing by which plants produce these molecules has been the focus of numerous studies. The tree species Hopea hainanensis generally produces green leaves at all growth stages; however, a few explored individuals have been identified possessing red leaves on the top of the seedlings at a young stage. While the phenomenon of leaf color varying with age has been studied in several species, the underlying mechanisms are largely unknown in H. hainanensis. Using a metabolomics approach, the young red leaves in H. hainanensis were found to contain higher levels of anthocyanins and flavonoids than the young green-leaved individuals. Among anthocyanins, pelargonidin and cyanidin were the most likely candidates contributing to the red color of the young leaves. Transcriptome results indicated the genes related to the production of these anthocyanins were significantly upregulated, leading to greater accumulation of red pigments. Specifically, the expression of several MYB and bHLH genes in young red leaf lines was significantly higher than that in the young green leaf lines, especially HhMYB66, HhMYB91, HhMYB6, and HhbHLH70. As such these four transcription factors are probably the main regulatory genes resulting in young red leaves in H. hainanensis. From these results, comparative analyses with other species can be made to better understand the evolution of pigment biosynthesis and how anthocyanins function in plant metabolism and evolution/adaptation.
Interspecific Neighbor Stimulates Peanut Growth Through Modulating Root Endophytic Microbial Community Construction
Authors: Chen Pin , He Wei , Shen Yi , Zhu Lingyue , Yao Xiangzhi , Sun Ruibo , Dai Chuanchao , Sun Bo , Chen Yan
Published: March 2022

Plants have evolved the capability to respond to interspecific neighbors by changing morphological performance and reshaping belowground microbiota. However, whether neighboring plants influence the microbial colonization of the host’s root and further affect host performance is less understood. In this study, using 16S rRNA high-throughput sequencing of peanut (Arachis hypogaea L.) roots from over 5 years of mono- and intercropping field systems, we found that neighbor maize can alter the peanut root microbial composition and re-shape microbial community assembly. Interspecific maize coexistence increased the colonization of genera Bradyrhizobium and Streptomyces in intercropped peanut roots. Through endophytic bacterial isolation and isolate back inoculation experiments, we demonstrated that the functional potentials of available nutrient accumulation and phytohormones production from Bradyrhizobium and Streptomyces endowed them with the ability to act as keystones in the microbial network to benefit peanut growth and production with neighbor competition. Our results support the idea that plants establish a plant-endophytic microbial holobiont through root selective filtration to enhance host competitive dominance, and provide a promising direction to develop modern diversified planting for harnessing crop microbiomes for the promotion of crop growth and productivity in sustainable agriculture.
Genome-Wide Association Mapping Indicates Quantitative Genetic Control of Spot Blotch Resistance in Bread Wheat and the Favorable Effects of Some Spot Blotch Loci on Grain Yield
Authors: Juliana Philomin , He Xinyao , Poland Jesse , Shrestha Sandesh , Joshi Arun K. , Huerta-Espino Julio , Govindan Velu , Crespo-Herrera Leonardo Abdiel , Mondal Suchismita , Kumar Uttam , Bhati Pradeep K. , Vishwakarma Manish , Singh Ravi P. , Singh Pawan K.
Published: March 2022

Spot blotch caused by the fungus Bipolaris sorokiniana poses a serious threat to bread wheat production in warm and humid wheat-growing regions of the world. Hence, the major objective of this study was to identify consistent genotyping-by-sequencing (GBS) markers associated with spot blotch resistance using genome-wide association mapping on a large set of 6,736 advanced bread wheat breeding lines from the International Maize and Wheat Improvement Center. These lines were phenotyped as seven panels at Agua Fria, Mexico between the 2013–2014 and 2019–2020 crop cycles. We identified 214 significant spot blotch associated GBS markers in all the panels, among which only 96 were significant in more than one panel, indicating a strong environmental effect on the trait and highlights the need for multiple phenotypic evaluations to identify lines with stable spot blotch resistance. The 96 consistent GBS markers were on chromosomes 1A, 1B, 1D, 2A, 3B, 4A, 5B, 5D, 6B, 7A, 7B, and 7D, including markers possibly linked to the Lr46, Sb1, Sb2 and Sb3 genes. We also report the association of the 2NS translocation from Aegilops ventricosa with spot blotch resistance in some environments. Moreover, the spot blotch favorable alleles at the 2NS translocation and two markers on chromosome 3BS (3B_2280114 and 3B_5601689) were associated with increased grain yield evaluated at several environments in Mexico and India, implying that selection for favorable alleles at these loci could enable simultaneous improvement for high grain yield and spot blotch resistance. Furthermore, a significant relationship between the percentage of favorable alleles in the lines and their spot blotch response was observed, which taken together with the multiple minor effect loci identified to be associated with spot blotch in this study, indicate quantitative genetic control of resistance. Overall, the results presented here have extended our knowledge on the genetic basis of spot blotch resistance in bread wheat and further efforts to improve genetic resistance to the disease are needed for reducing current and future losses under climate change.
Corylus avellana L. Aroma Blueprint: Potent Odorants Signatures in the Volatilome of High Quality Hazelnuts
Authors: Squara Simone , Stilo Federico , Cialiè Rosso Marta , Liberto Erica , Spigolon Nicola , Genova Giuseppe , Castello Giuseppe , Bicchi Carlo , Cordero Chiara
Published: March 2022

The volatilome of hazelnuts (Corylus avellana L.) encrypts information about phenotype expression as a function of cultivar/origin, post-harvest practices, and their impact on primary metabolome, storage conditions and shelf-life, spoilage, and quality deterioration. Moreover, within the bulk of detectable volatiles, just a few of them play a key role in defining distinctive aroma (i.e., aroma blueprint) and conferring characteristic hedonic profile. In particular, in raw hazelnuts, key-odorants as defined by sensomics are: 2,3-diethyl-5-methylpyrazine (musty and nutty); 2-acetyl-1,4,5,6-tetrahydropyridine (caramel); 2-acetyl-1-pyrroline (popcorn-like); 2-acetyl-3,4,5,6-tetrahydropyridine (roasted, caramel); 3-(methylthio)-propanal (cooked potato); 3-(methylthio)propionaldehyde (musty, earthy); 3,7-dimethylocta-1,6-dien-3-ol/linalool (citrus, floral); 3-methyl-4-heptanone (fruity, nutty); and 5-methyl-(E)-2-hepten-4-one (nutty, fruity). Dry-roasting on hazelnut kernels triggers the formation of additional potent odorants, likely contributing to the pleasant aroma of roasted nuts. Whiting the newly formed aromas, 2,3-pentanedione (buttery); 2-propionyl-1-pyrroline (popcorn-like); 3-methylbutanal; (malty); 4-hydroxy-2,5-dimethyl-3(2H)-furanone (caramel); dimethyl trisulfide (sulfurous, cabbage) are worthy to be mentioned. The review focuses on high-quality hazelnuts adopted as premium primary material by the confectionery industry. Information on primary and secondary/specialized metabolites distribution introduces more specialized sections focused on volatilome chemical dimensions and their correlation to cultivar/origin, post-harvest practices and storage, and spoilage phenomena. Sensory-driven studies, based on sensomic principles, provide insights on the aroma blueprint of raw and roasted hazelnuts while robust correlations between non-volatile precursors and key-aroma compounds pose solid foundations to the conceptualization of aroma potential.
Chromosome-Level Genome Assembly for Acer pseudosieboldianum and Highlights to Mechanisms for Leaf Color and Shape Change
Authors: Li Xiang , Cai Kewei , Han Zhiming , Zhang Shikai , Sun Anran , Xie Ying , Han Rui , Guo Ruixue , Tigabu Mulualem , Sederoff Ronald , Pei Xiaona , Zhao Chunli , Zhao Xiyang
Published: March 2022

Acer pseudosieboldianum (Pax) Komarov is an ornamental plant with prominent potential and is naturally distributed in Northeast China. Here, we obtained a chromosome-scale genome assembly of A. pseudosieboldianum combining HiFi and Hi-C data, and the final assembled genome size was 690.24 Mb and consisted of 287 contigs, with a contig N50 value of 5.7 Mb and a BUSCO complete gene percentage of 98.4%. Genome evolution analysis showed that an ancient duplication occurred in A. pseudosieboldianum. Phylogenetic analyses revealed that Aceraceae family could be incorporated into Sapindaceae, consistent with the present Angiosperm Phylogeny Group system. We further construct a gene-to-metabolite correlation network and identified key genes and metabolites that might be involved in anthocyanin biosynthesis pathways during leaf color change. Additionally, we identified crucial teosinte branched1, cycloidea, and proliferating cell factors (TCP) transcription factors that might be involved in leaf morphology regulation of A. pseudosieboldianum, Acer yangbiense and Acer truncatum. Overall, this reference genome is a valuable resource for evolutionary history studies of A. pseudosieboldianum and lays a fundamental foundation for its molecular breeding.
Effects of Pulsed-Wave Chromotherapy and Guided Relaxation on the Theta-Alpha Oscillation During Arrest Reaction
Authors: Cheron Guy , Ristori Dominique , Petieau Mathieu , Simar Cédric , Zarka David , Cebolla Ana-Maria
Published: March 2022

The search for the best wellness practice has promoted the development of devices integrating different technologies and guided meditation. However, the final effects on the electrical activity of the brain remain relatively sparse. Here, we have analyzed of the alpha and theta electroencephalographic oscillations during the realization of the arrest reaction (AR; eyes close/eyes open transition) when a chromotherapy session performed in a dedicated room [Rebalance (RB) device], with an ergonomic bed integrating pulsed-wave light (PWL) stimulation, guided breathing, and body scan exercises. We demonstrated that the PWL induced an evoked-related potential characterized by the N2-P3 components maximally recorded on the fronto-central areas and accompanied by an event-related synchronization (ERS) of the delta–theta–alpha oscillations. The power of the alpha and theta oscillations was analyzed during repeated ARs testing realized along with the whole RB session. We showed that the power of the alpha and theta oscillations was significantly increased during the session in comparison to their values recorded before. Of the 14 participants, 11 and 6 showed a significant power increase of the alpha and theta oscillations, respectively. These increased powers were not observed in two different control groups (n = 28) who stayed passively outside or inside the RB room but without any type of stimulation. These preliminary results suggest that PWL chromotherapy and guided relaxation induce measurable electrical brain changes that could be beneficial under neuropsychiatric perspectives.
Effects of Priming Discriminated Experiences on Emotion Recognition Among Asian Americans
Authors: Chang Sophia , Kang Sun-Mee
Published: March 2022

This study explored the priming effects of discriminated experiences on emotion recognition accuracy of Asian Americans. We hypothesized that when Asian Americans were reminded of discriminated experiences due to their race, they would detect subtle negative emotional expressions on White faces more accurately than would Asian Americans who were primed with a neutral topic. This priming effect was not expected to emerge in detecting negative facial expressions on Asian faces. To test this hypothesis, 108 participants were randomly assigned to one of two conditions: write about their experiences with racial discrimination (experimental) or write about a neutral topic (control). Then, they were given an emotion recognition test consisting of White and Asian faces. The current study found a significant interaction effect of priming condition by target race. When Asian Americans were reminded of discriminated experiences, they displayed heightened sensitivity to negative emotional expressions on White faces, but not to the negative expressions on Asian faces. The implications of these findings were discussed.
The Interaction Between Morphological Awareness and Word Detection Skills in Predicting Speeded Passage Reading in Primary and Secondary School Chinese Readers
Authors: Liu Duo , Xu Zhengye , Wang Li-Chih
Published: March 2022

Previous studies suggest that morphological awareness (MA) and word detection skills have facilitating roles in reading fluency; however, it is unknown whether they can interplay with each other in such roles. The present study explored the relationships of MA, word detection, and passage reading fluency across ages. In total, 180 Chinese primary and secondary school students, aged from 8.52 to 15.67 years, completed tasks for these aforementioned capacities. After controlling gender, non-verbal intelligence, and reading ability at the word level, the results showed that the participants with higher scores for MA or word detection performed better in passage reading fluency. However, the predictive effect of word detection on reading fluency became weaker as the children became older. The interaction between MA and word detection was positive in younger children, whereas this interaction tended to be negative for older children. The results demonstrated a dynamic interplay between MA and word detection in contributing to passage reading fluency in Chinese children. While it has a positive interaction with word detection on reading fluency in younger children, MA may become a compensator in older children (e.g., over 14 years old) whose word detection skills are less effective in facilitating fluent reading.
Multiple Roles of Grit in the Relationship Between Interpersonal Stress and Psychological Security of College Freshmen
Authors: Yang Qingsong , Shi Mengxi , Tang Dandan , Zhu Hai , Xiong Ke
Published: March 2022

Grit, as an important positive psychological quality, has rarely been studied for its role involved in the mechanism between stress and psychological security. This article explores the moderating and mediating role of grit in the relationship between interpersonal stress and psychological security of freshmen through two studies. In study 1, freshmen from several Chinese universities (N = 1,224) were recruited to complete a battery of questionnaire, including assessments about interpersonal stress, grit, and psychological security. The moderating effect analysis showed that grit moderated the relationship between interpersonal stress and psychological security. Specifically, grit buffered the negative effects of interpersonal stress on freshmen’s psychological security, but this effect was obvious only when the level of interpersonal stress was relatively low, and decreased when the level of interpersonal stress was high. In study 2, college freshmen from another university apart from above ones (N = 604) were recruited, and we verified the results of study 1 and further explored the mediating role of grit in the relationship between interpersonal stress and security. The moderating effect analysis of study 2 also verified that of study 1. The mediating effect analysis showed that interpersonal stress not only negatively predicted psychological security, but also affected psychological security through the mediation of grit. In general, grit played a mediating and moderating role in the relationship between interpersonal stress and psychological security. This study provides first-hand evidence to explain the multiple roles of grit in the relationship between interpersonal stress and psychological security.
Does It Matter If Students (Dis)like School? Associations Between School Liking, Teacher and School Connectedness, and Exclusionary Discipline
Authors: Graham Linda J. , Gillett-Swan Jenna , Killingly Callula , Van Bergen Penny
Published: March 2022

School liking is an important factor in student engagement, well-being, and academic achievement, but it is also potentially influenced by factors external to the individual, such as school culture, teacher support, and approaches to discipline. The present study employed a survey methodology to investigate the associations between school liking and disliking, teacher and school connectedness, and experiences of exclusionary discipline from the perspective of students themselves. Participants included 1,002 students (Grades 7–10) from three secondary schools serving disadvantaged communities. Results indicated clear differences between students who like and dislike school in terms of their preferred school activities and school disciplinary history, with students who disliked school experiencing overall lower school connectedness. Moreover, students who disliked school experienced less positive relationships with their teachers, and this was even more pronounced for students who had been previously suspended. The findings reveal key differences between students who do and do not like school, differences that may be masked by typical research approaches. This research indicates the need for more nuanced, student-informed approaches to inclusive school reform.
Motor Imagery and Action Observation as Appropriate Strategies for Home-Based Rehabilitation: A Mini-Review Focusing on Improving Physical Function in Orthopedic Patients
Authors: Paravlic Armin H.
Published: March 2022

Dynamic stability of the knee and weakness of the extensor muscles are considered to be the most important functional limitations after anterior cruciate ligament (ACL) injury, probably due to changes at the central (cortical and corticospinal) level of motor control rather than at the peripheral level. Despite general technological advances, fewer contraindicative surgical procedures, and extensive postoperative rehabilitation, up to 65% of patients fail to return to their preinjury level of sports, and only half were able to return to competitive sport. Later, it becomes clear that current rehabilitation after knee surgery is not sufficient to address the functional limitations after ACL reconstruction even years after surgery. Therefore, new therapeutic tools targeting the central neural system, i.e., the higher centers of motor control, should be investigated and integrated into current rehabilitation practice. To improve motor performance when overt movement cannot be fully performed (e.g., due to pain, impaired motor control, and/or joint immobilization), several techniques have been developed to increase physical and mental activation without the need to perform overt movements. Among the most popular cognitive techniques used to increase physical performance are motor imagery and action observation practices. This review, which examines the available evidence, presents the underlying mechanisms of the efficacy of cognitive interventions and provides guidelines for their use at home.
Predicting College Students’ Bike-Sharing Intentions Based on the Theory of Planned Behavior
Authors: Chen Xiaofang
Published: March 2022

Shared bicycles are sustainable and effective transportation tools in college campuses. Accordingly, this study aimed to assess the behavioral intention of college students toward bike-sharing as an environmentally friendly and social mode of travel. It applied the Theory of Planned Behavior framework to a bike-sharing context and explored the impact of perceived benefits and government policy on college students’ bike-sharing usage. A survey of 934 college students was conducted in Zhejiang province to test the proposed model, and 782 were valid. The findings pointed out that attitude, subjective norms, and perceived behavioral control have a significant and positive impact on college students’ intentions toward bike-sharing. Meanwhile, the empirical results revealed that perceived benefits and government policy were the important factors driving college students’ intention and behavior for bike-sharing usage. Moreover, the results ascertained that the intention was aligned with actual actions; eventually, some targeted managerial implications are presented. This study enhances the current understanding of the usage behavior of college students in bike-sharing and provides timely insights for government policymakers and enterprise operators to promote sustainable bike-sharing practices in China and other countries.
The Mediating Role of Chinese College Students’ Control Strategies: Belief in a Just World and Life History Strategy
Authors: Lin Xuanxuan , Wang Rongzhao , Huang Tao , Gao Hua
Published: March 2022

The harshness and unpredictability of early life circumstances shape life history strategies for trade-offs between the resources devoted to somatic and reproductive efforts of individuals in the developmental process. This paper uses belief in a just world as a reflection of early environmental cues to predict an individual’s life history strategies. Research has found that belief in a just world influences life history strategies through a sense of control. However, the relationship between a sense of control and a life history strategy is flawed because influencing life history strategies should be intrinsic to control strategies rather than a sense of control. A total of 408 Chinese undergraduate students completed the Personal Belief in a Just World Scale, Mini-K Scale, and Primary and Secondary Control Scale. Structural equation modeling suggested that belief in a just world can directly or indirectly influence life history strategies through primary and secondary control strategies, respectively; there was no statistical difference in the degree of influence between the two paths. These results deepen our understanding of the underlying mechanisms in the relationship between belief in a just world and life history strategies, which can be utilized to ensure a slow life history strategy among Chinese university students in the future.
A Web-Based Prediction Model for Cancer-Specific Survival of Elderly Patients With Clear Cell Renal Cell Carcinoma: A Population-Based Study
Authors: Zhanghuang Chenghao , Wang Jinkui , Zhang Zhaoxia , Jin Liming , Tan Xiaojun , Mi Tao , Liu Jiayan , Li Mujie , He Dawei
Published: March 2022

Background

Clear cell renal cell carcinoma (ccRCC) is expected in the elderly and poor prognosis. We aim to explore prognostic factors of ccRCC in the elderly and construct a nomogram to predict cancer-specific survival (CSS) in elderly patients with ccRCC.

Methods

Clinicopathological information for all elderly patients with ccRCC from 2004 to 2018 was downloaded from the Surveillance, Epidemiology, and End Results (SEER) program. All patients were randomly assigned to a training cohort (70%) or a validation cohort (30%). Univariate and multivariate Cox regression models were used to identify the independent risk factors for CSS. A new nomogram was constructed to predict CSS at 1-, 3-, and 5 years in elderly patients with ccRCC based on independent risk factors. Subsequently, we used the consistency index (C-index), calibration curves, and the area under the receiver operating curve (AUC) and decision curve analysis (DCA) to test the prediction accuracy of the model.

Results

A total of 33,509 elderly patients with ccRCC were enrolled. Univariate and multivariate Cox regression analyses results showed that age, sex, race, marriage, tumor size, histological grade, tumor, nodes, and metastases (TNM) stage, and surgery were independent risk factors for CSS in elderly patients with ccRCC. We constructed a nomogram to predict CSS in elderly patients with ccRCC. The C-index of the training cohort and validation cohort was 0.81 (95% CI: 0.802–0.818) and 0.818 (95% CI: 0.806–0.830), respectively. The AUC of the training cohort and validation cohort also suggested that the prediction model had good accuracy. The calibration curve showed that the observed value of the prediction model was highly consistent with the predicted value. DCA showed good clinical application value of the nomogram.

Conclusion

In this study, we explored prognostic factors in elderly patients with ccRCC. We found that age, sex, marriage, TNM stage, surgery, and tumor size were independent risk factors for CSS. We constructed a new nomogram to predict CSS in elderly patients with ccRCC with good accuracy and reliability, providing clinical guidance for patients and physicians.
Genomic Snapshot of SARS-CoV-2 in Migrants Entering Through Mediterranean Sea Routes
Authors: Grandi Nicole , Paglietti Bianca , Cusano Roberto , Ibba Gabriele , Lai Vincenzo , Piu Claudia , Angioj Flavia , Serra Caterina , Kelvin David J. , Tramontano Enzo , Rubino Salvatore
Published: March 2022

In December 2019, a novel coronavirus emerged in Wuhan, China, rapidly spreading into a global pandemic. Italy was the first European country to experience SARS-CoV-2 epidemic, and one of the most severely affected during the first wave of diffusion. In contrast to the general restriction of people movements in Europe, the number of migrants arriving at Italian borders via the Mediterranean Sea route in the summer of 2020 had increased dramatically, representing a possible, uncontrolled source for the introduction of novel SARS-CoV-2 variants. Importantly, most of the migrants came from African countries showing limited SARS-CoV-2 epidemiological surveillance. In this study, we characterized the SARS-CoV-2 genome isolated from an asymptomatic migrant arrived in Sardinia via the Mediterranean route in September 2020, in comparison with SARS-CoV-2 isolates arrived in Sicily through the Libyan migration route; with SARS-CoV-2 isolates circulating in Sardinia during 2020; and with viral genomes reported in African countries during the same summer. Results showed that our sequence is not phylogenetically related to isolates from migrants arriving in Sicily, nor to isolates circulating in Sardinia territory, having greater similarity to SARS-CoV-2 genomes reported in countries known for being sites of migrant embarkation to Italy. This is in line with the hypothesis that most SARS-CoV-2 infections among migrants have been acquired prior to embarking to Italy, possibly during the travel to or the stay in crowded Libyan immigrant camps. Overall, these observations underline the importance of dedicated SARS-CoV-2 surveillance of migrants arriving in Italy and in Europe through the Mediterranean routes.
Implications of Sphingolipids on Aging and Age-Related Diseases
Authors: Li Shengxin , Kim Hyun-Eui
Published: March 2022

Aging is a process leading to a progressive loss of physiological integrity and homeostasis, and a primary risk factor for many late-onset chronic diseases. The mechanisms underlying aging have long piqued the curiosity of scientists. However, the idea that aging is a biological process susceptible to genetic manipulation was not well established until the discovery that the inhibition of insulin/IGF-1 signaling extended the lifespan of C. elegans. Although aging is a complex multisystem process, López-Otín et al. described aging in reference to nine hallmarks of aging. These nine hallmarks include: genomic instability, telomere attrition, epigenetic alterations, loss of proteostasis, deregulated nutrient sensing, mitochondrial dysfunction, cellular senescence, stem cell exhaustion, and altered intercellular communication. Due to recent advances in lipidomic, investigation into the role of lipids in biological aging has intensified, particularly the role of sphingolipids (SL). SLs are a diverse group of lipids originating from the Endoplasmic Reticulum (ER) and can be modified to create a vastly diverse group of bioactive metabolites that regulate almost every major cellular process, including cell cycle regulation, senescence, proliferation, and apoptosis. Although SL biology reaches all nine hallmarks of aging, its contribution to each hallmark is disproportionate. In this review, we will discuss in detail the major contributions of SLs to the hallmarks of aging and age-related diseases while also summarizing the importance of their other minor but integral contributions.
Role of BAG5 in Protein Quality Control: Double-Edged Sword?
Authors: Gupta Manish K. , Randhawa Puneet Kaur , Masternak Michal M.
Published: March 2022

Cardiovascular disorder is the major health burden and cause of death among individuals worldwide. As the cardiomyocytes lack the ability for self-renewal, it is utmost necessary to surveil the protein quality in the cells. The Bcl-2 associated anthanogene protein (BAG) family and molecular chaperones (HSP70, HSP90) actively participate in maintaining cellular protein quality control (PQC) to limit cellular dysfunction in the cells. The BAG family contains a unique BAG domain which facilitates their interaction with the ATPase domain of the heat shock protein 70 (HSP70) to assist in protein folding. Among the BAG family members (BAG1-6), BAG5 protein is unique since it has five domains in tandem, and the binding of BD5 induces certain conformational changes in the nucleotide-binding domain (NBD) of HSP70 such that it loses its affinity for binding to ADP and results in enhanced protein refolding activity of HSP70. In this review, we shall describe the role of BAG5 in modulating mitophagy, endoplasmic stress, and cellular viability. Also, we have highlighted the interaction of BAG5 with other proteins, including PINK, DJ-1, CHIP, and their role in cellular PQC. Apart from this, we have described the role of BAG5 in cellular metabolism and aging.
Systems Analysis Reveals Contraceptive-Induced Alteration of Cervicovaginal Gene Expression in a Randomized Trial
Authors: Balle Christina , Gupta Prachi M. , Tharp Gregory K. , Nelson Sydney A. , Konstantinus Iyaloo N. , Lennard Katie , Jaumdally Shameem Z. , Happel Anna-Ursula , Barnabas Shaun L. , Gill Katherine , Bekker Linda-Gail , Passmore Jo-Ann S. , Jaspan Heather B. , Bosinger Steven E.
Published: March 2022

Hormonal contraceptives (HCs) are vital in managing the reproductive health of women. However, HC usage has been linked to perturbations in cervicovaginal immunity and increased risk of sexually transmitted infections. Here, we evaluated the impact of three HCs on the cervicovaginal environment using high-throughput transcriptomics. From 2015 to 2017, 130 adolescent females aged 15–19 years were enrolled into a substudy of UChoose, a single-site, open-label randomized, crossover trial (NCT02404038) and randomized to injectable norethisterone–enanthate (Net-En), combined oral contraceptives (COC), or etonorgesterol/ethinyl–estradiol–combined contraceptive vaginal ring (CCVR). Cervicovaginal samples were collected after 16 weeks of randomized HC use and analyzed by RNA-Seq, 16S rRNA gene sequencing, and Luminex analysis. Participants in the CCVR arm had a significant elevation of transcriptional networks driven by IL-6, IL-1, and NFKB, and lower expression of genes supporting epithelial barrier integrity. An integrated multivariate analysis demonstrated that networks of microbial dysbiosis and inflammation best discriminated the CCVR arm from the other contraceptive groups, while genes involved in epithelial cell differentiation were predictive of the Net-En and COC arms. Collectively, these data from a randomized trial represent the most comprehensive “omics” analyses of the cervicovaginal response to HCs and provide important mechanistic guidelines for the provision of HCs in sub-Saharan Africa.
The Farm-to-Fork Journey: Keeping Produce Fresh and Safe to Eat
Authors: Pierce Alice , Student Joseph , Melotto Maeli
Published: March 2022

Fruits and vegetables are nutritious and help you stay healthy. Yet, they might not be safe to eat right after you purchase them. The farm-to-fork journey includes all the stages your produce goes through, from growing the plants to being eaten in your home. It is important to maintain freshness of the fruits and vegetables and prevent microbial contamination of produce throughout the farm-to-fork journey. At home, you must follow the guidelines provided on the food’s packaging, or simply wash your fruits and vegetables well. In this article, we will walk you through five stages in the farm-to-fork journey: production, processing, distribution, retail, and consumer. We will also discuss where food can become contaminated. At the end of the article, we will give you some ideas of what you can do at home to prevent getting sick.
The Relationship Between Dietary Intake and Sleep Quality in Endurance Athletes
Authors: Moss Kamiah , Zhang Yan , Kreutzer Andreas , Graybeal Austin J. , Porter Ryan R. , Braun-Trocchio Robyn , Shah Meena
Published: March 2022

Many endurance athletes have poor sleep quality which may affect performance and health. It is unclear how dietary intake affects sleep quality among athletes. We examined if sleep quality in endurance athletes is associated with consumption of fruit, vegetables, whole grains, dairy milk, and caffeinated beverages. Two hundred thirty-four endurance athletes (39.5 ± 14.1 year) participated in a survey. Participants provided information on demographics, anthropometry, sleep behavior and quality, and dietary intake via questionnaires. Sleep quality was assessed using the Athlete Sleep Screening Questionnaire (ASSQ) with a global score (ASSQ-global) and subscales including sleep difficulty (ASSQ-SD), chronotype (ASSQ-C), and disordered breathing while sleeping (ASSQ-SDB). A general linear model (GLM), adjusted for age, body mass index, sleep discomfort, sleep behavior, gender, race, and ethnicity, showed that higher caffeinated beverage intake was related to poorer global sleep quality (p = 0.01) and increased risk for disordered breathing while sleeping (p = 0.03). Higher whole grain intake was associated with a morning chronotype and lower risk for sleep issues (p = 0.01). The GLM did not reveal a relationship between sleep quality and dairy milk, fruit, and vegetable intake. In conclusion, caffeinated beverages and whole grain intake may influence sleep quality. This relationship needs to be confirmed by further research.
Leveling the Playing Field: A New Proposed Method to Address Relative Age- and Maturity-Related Bias in UK Male Academy Soccer Players
Authors: Bolckmans Sofie , Starkes Janet L. , Towlson Chris , Barnes Chris , Parkin Guy , Helsen Werner F.
Published: March 2022

Relative age selection bias persists within all major soccer leagues and youth soccer academies across the globe, with the relative age effect (RAE) being typically characterized as the over selection of relatively older players (who have sometimes also been shown to be early maturing). The aim of this study was to examine if a new allocation method (i) eliminates the RAE, and (ii) reduces the presence of any additional maturity-related differences in anthropometric and physical fitness characteristics which may exist between players within the same selection category. In the first phase, 1,003 academy soccer players [under (U) 9–16] from 23 UK professional soccer clubs were sampled and a clear RAE per birth quarter (Q) was observed for the overall sample (Q1 = 45.0% vs. Q4 = 9.8%) as well as for the different age categories. Using the newly suggested reallocation method, youth players were divided by allocation date which was defined as the midway point between the chronological age and the estimated developmental (ED) birthdate. Stature was used as an anthropometric reference point on the P50 of the growth curve to determine the developmental birthdate for this new method. After the reallocation of the players using ED, the distribution of players was more equally spread (Q1 = 25.3%, Q2 = 25.6%, Q3 = 22.4%, Q4 = 26.7%). After reallocation, the mean delta stature was reduced by 16.6 cm (from 40.3 ± 7.08 to 23.7 ± 4.09 cm, d = 2.87). The mean delta body mass difference after allocation was reduced by 6.7kg (from 33.2 ± 6.39 to 26.5 ± 4.85 kg, d = 1.18). The mean age difference increased from 1.8 to 3.9 years. A total of 42.7% of the sample would have been reallocated to a different age group compared to the current one. After reallocation, 45% of the anthropometric and physical fitness comparisons showed reductions in the within-group variation expressed as a percentage of coefficient of variation (CV%). The U10 players demonstrated the largest reduction in CV% (−7.6%) of the anthropometric characteristics. The U10 squad also showed the largest reduction in CV% for various physical fitness characteristics (−7.5%). By both eliminating the RAE and reducing temporary maturity-related anthropometric and physical fitness differences, soccer academies across the world may diversify and increase the size of the talent pool both for clubs and national youth teams. In conclusion, this study provides further evidence that the newly proposed allocation method shows the potential to remove the RAE and to create a more “leveled playing field” by reducing the within-group variation of anthropometric and physical fitness characteristics affording relatively younger, and eventually, late-maturing players the opportunity to develop their talent fairly.
The Modified THP-1 Activation Assay for the In Vitro Identification of Drug-Inducing Systemic Hypersensitivity
Authors: Iulini Martina , Maddalon Ambra , Galbiati Valentina , Corsini Emanuela
Published: March 2022

The development of new low molecular weight drugs has many chances of failure and is an expensive process. Currently, there are no screening methods and/or models to assess the hazard of hypersensitivity reactions to drugs (DHRs) in the preclinical phase. DHRs represent 6–15% of adverse drug reactions. Although rare, DHRs represent a serious health problem for predisposed individuals, resulting, in some cases, in life-threatening pathologies. To date, there are no in vitro or in vivo sensitive models able to predict the sensitizing potential of drugs in the preclinical tests, and these reactions are highlighted only after the drug has been placed on the market, affecting both population and public health. This article describes a novel approach methodology for the study of the sensitizing potential of drugs based on the use of the human promyelocytic cell line THP-1 as a surrogate for dendritic cells. The method is based on the upregulation of specific surface markers (CD86 and CD54) and on the production of IL-8. In our experience, the THP-1 activation assay allowed the correct identification of drugs known to induce systemic hypersensitivity in humans, including the one associated with specific HLAs. This method may help to discover possible systemic hypersensitivity reactions early in the preclinical phase of drug development.
Empowering Veterinarians to Be Planetary Health Stewards Through Policy and Practice
Authors: Kiran Dilara , Sander William E. , Duncan Colleen
Published: March 2022

Veterinarians are established public health professionals, committing to promote public health when they take their veterinary oath. The issue of climate change and its impact on planetary health is vital to public health, and therefore, it is critical that climate change is regarded as within the veterinary scope of practice. However, climate change is a multi-faceted issue which requires interdisciplinary collaboration and integrated stakeholder involvement in order to establish effective solutions and impactful policies. As a result, in this perspective, we discuss how policy is critical to support veterinarians in the climate change space and argue that more explicit support is needed for veterinarians to take an active role in climate change adaption, resilience, and mitigation. We address the discrepancies between the human health and veterinary professions with respect to providing policy support and capacity for practitioners to be stewards to promote planetary health and shed light on the lack of veterinary capacity in this area. We stress that veterinary professional societies are well equipped to bolster their policies, expand education for veterinary professionals and students in policy and advocacy, and establish calls to action to address climate change and planetary health issues. Ultimately, as public health professionals, veterinarians are uniquely poised to be contributors to climate change solutions and they should be actively involved in policy decision-making and empowered to take active roles in interdisciplinary conversations surrounding this important issue.
Economic Vulnerability among Girls at Risk for Adolescent Pregnancy: Qualitative Findings among a Clinic Sample of Girls Residing in the U.S.–Mexico Border Region
Authors: Reed Elizabeth , Salazar Marissa , Behar Alma I. , Servin Argentina E. , Ayala Guadalupe X. , Silverman Jay G. , Rusch Melanie L. A. , Zuniga Mari L. , Raj Anita
Published: March 2022

Background: In the U.S., research to identify the risk factors explaining high rates of adolescent pregnancy disproportionately affecting racial/ethnic minorities, including Latinas, have largely focused on social and cultural factors that influence girls’ pregnancy intentions and decisions regarding the use of contraception, as well as girls’ sexual and reproductive decision-making control in relationships. However, economic factors may play a role in increasing girls’ risk for adolescent pregnancy as well. Disproportionately high rates of adolescent pregnancy occur in areas of concentrated poverty, with higher rates among ethnic minorities. This qualitative study used a descriptive, exploratory design with a content analysis approach (1) to describe economic vulnerability in girls’ lives and (2) to identify potential ways in which economic vulnerability may influence risk factors for adolescent pregnancy among adolescent females at high risk for pregnancy recruited from a health clinic by the U.S.–Mexico border in California. Methods: Qualitative in-depth interviews were conducted among 21 girls reporting risk factors for pregnancy (including a previous pregnancy, experiences of dating violence, or having ever been diagnosed with an STI) and who were seeking sexual/reproductive health services at a clinic near the U.S.–Mexico border in California. Participants were asked about their family life, economic stressors, characteristics of intimate partner relationships, and future education/career aspirations. Interviews were analyzed using content analysis to identify common themes related to economic vulnerability and risk for pregnancy. Results: Female participants were 17 years of age on average, most (72%) were Latina, and over half (60%) were in a relationship. Participants reported a high level of economic vulnerability at home, which they felt compromised their welfare, negatively affected expectations for future educational goals, and promoted financial dependence for basic needs (e.g., food) on male partners. The latter often compromised girls’ decision-making control with relationship partners over contraceptive use and pregnancy timing. Conclusions: Study findings suggest that economic vulnerability: (a) supports economic reliance on male partners, with implications for male partner control over pregnancy decisions and (b) hinders girls’ expectations for future educational opportunities, which may reduce girls’ prioritization to delay pregnancy.
The Influence of Country Risks on the International Agricultural Trade Patterns Based on Network Analysis and Panel Data Method
Authors: Sun Qingru , Hou Meiyi , Shi Shuaiwei , Cui Liwei , Xi Zenglei
Published: March 2022

The pattern of international agricultural trade is undergoing profound changes. The influence of country risks on the international agricultural trade pattern is prominent. In this paper, we comprehensively analyze the international agricultural trade patterns and explore the influence of country risks on them. Specifically, we first construct an international agricultural trade network (IATN) based on complex network theory. Second, we analyze each country’s diversity of import sources and the position of countries in the IATN using the Herfindahl–Hirschman Index (HHI) and network indicators, such as in-degree, out-degree, weighted in-degree, weighted out-degree, and betweenness centrality. Third, this paper explores the influence of different types of country risks, including economic risk and political risk, on international agricultural trade patterns using the panel regression method. The results show that countries played different roles and occupied different positions in the international agricultural trade pattern; notably, the United States occupied a core position, while Japan and Mexico had insufficient diversity in import sources. Moreover, based on the panel regression method, we find that political risks have a positive impact on the agricultural trade pattern, while an unstable economic environment could inhibit the agricultural trade pattern in various countries. This study could provide references for countries to implement agricultural trade policies regarding country risks to ensure stable agricultural trade relations and national food security.