bluuebunny/crossref_metadata_2025 huggingface dataset:
Antimicrobial Susceptibility of Escherichia coli and ESBL-Producing Escherichia coli Diffusion in Conventional, Organic and Antibiotic-Free Meat Chickens at Slaughter
Authors: Musa Laura , Casagrande Proietti Patrizia , Branciari Raffaella , Menchetti Laura , Bellucci Sara , Ranucci David , Marenzoni Maria Luisa , Franciosini Maria Pia
Published: July 2020

As a result of public health concerns regarding antimicrobial resistance in animal-based food products, conventional poultry companies have turned to ‘raised without antibiotics’ (ABF) and organic farming systems. In this work, we evaluated the influence of rearing systems on antimicrobial susceptibility in E. coli and extended-spectrum β-lactamase (ESLB) E. coli diffusion in conventional (C), organic (O) and antibiotic free (ABF) chicken samples collected from cloacal swabs and skin samples in slaughterhouse. The E. coli isolates from conventional (135), antibiotic-free (131) and organic (140) samples were submitted to the Kirby–Bauer method and ESBL E. coli were analyzed by the microdilution test. Conventional samples showed the highest number of strains resistant to ampicillin (89.6%; p < 0.01), cefotaxime (43.7%; p < 0.01), nalidixic acid (57.8%; p < 0.01), ciprofloxacin (44.4%; p < 0.001), and trimethoprim/sulfamethoxazole (62.2%; p < 0.01), with patterns of multi-resistance to three (35.1%) and to four antimicrobials (31.3%), whereas most of the E. coli isolated from antibiotic-free and organic chicken samples revealed a co-resistance pattern (29.2% and 39%, respectively). The highest number of ESBL E. coli was observed in conventional, in both cloacal and skin samples and the lowest in organic (p < 0.001). Our results are consistent with the effect of conventional farming practices on E. coli antimicrobial resistance and ESBL E. coli number, due to the use of antimicrobials and close contact with litter for most of the production cycle.
Dynamics of the Inbreeding Coefficient and Homozygosity in Thoroughbred Horses in Russia
Authors: Kalashnikov Valery , Khrabrova Lyudmila , Blohina Nina , Zaitcev Alexander , Kalashnikova Tatyana
Published: July 2020

The Thoroughbred (TB) horse has hugely impacted the development of horse breeding around the world. This breed has unique genetic qualities due to having had a closed studbook for approximately 300 years. In Russia, TBs have been bred since the second half of the 18th century. Here, we analyzed the genetic diversity and the inbreeding level in TB horses (n = 9680) for the period from 1990 to 2018 using polymorphisms of 17 microsatellite loci. We found that the genetic structure of the TB breed in Russia is represented by 100 alleles of panel STR (short tandem repeat) loci and has been stable for the past three decades. The conducted monitoring revealed a slight increase in the Wright’s inbreeding coefficient in all age and sex groups of TB horses (stallions, broodmares, and foals) from 0.68% to 0.90%, which was followed by a decrease in the degree of heterozygosity, Ho, from 68.5% to 67.6%. The Spearman’s rank correlation coefficient between the level of inbreeding and the degree of homozygosity was estimated (r = 0.022; p > 0.05). The obtained data on the DNA genotypes of horses of different breeds provide a unique base for the evaluation of genetic variability and the control of genetic variability of horses in selection programs.
Attitudes and Beliefs of Eastern European Consumers Towards Animal Welfare
Authors: Tomasevic Igor , Bahelka Ivan , Čítek Jaroslav , Čandek-Potokar Marjeta , Djekić Ilija , Getya Andriy , Guerrero Luis , Ivanova Sonya , Kušec Goran , Nakov Dimitar , Sołowiej Bartosz , Stoica Maricica , Szabó Csaba , Tudoreanu Liliana , Weiler Ulrike , Font-i-Furnols Maria
Published: July 2020

The aim of this exploratory work, because of the existing bias on the size of the sample and some of the sociodemographic characteristics of the participants, was to investigate the Eastern European consumers’ beliefs and attitudes toward animal welfare, to perform a cross-country segmentation analysis and to observe possible differences with their Western European counterparts. For this purpose, a survey was conducted with 5508 consumers from 13 Eastern European countries (Bosnia and Herzegovina, Bulgaria, Czech Republic, Croatia, North Macedonia, Hungary, Moldova, Poland, Romania, Serbia, Slovakia, Slovenia, and Ukraine) using a questionnaire with nine statements about consumers beliefs regarding animal welfare (aspects of management, ethical issues about animals, and consequences of animal welfare on meat quality and price), one statement about the willingness to pay more for meat produced under better welfare conditions, and four statements regarding attitudes toward animal welfare. Differences between countries were detected for all the statements. Moreover, three clusters of consumers were identified: one with consumers indifferent towards animal welfare; one with consumers concerned about animal welfare, but they believe it is difficult to achieve; and one with consumers concerned about animal welfare, and they believe it is possible to achieve it.
A Preliminary Study toward a Rapid Assessment of Age-Related Behavioral Differences in Family Dogs
Authors: Kubinyi Eniko , Iotchev Ivaylo B.
Published: July 2020

Over the last few years, several efforts have been undertaken to characterize the aging process in dogs. In the present study, we evaluate a short protocol measuring dogs’ cognitive, social, and physical capacities. Our aim was to develop a feasible test battery, with minimal pre-training requirements, no complex devices, and which is set outdoors (i.e., a specific testing room is not needed). As ageing in dogs is usually associated with a decrease in activity, we also assessed the personality trait activity/excitability with a dog personality questionnaire. Four subtests proved sensitive to the dogs’ age. In particular, old dogs displayed less approaching and following behaviors toward an unknown but friendly human, showed both less avoidance and interest toward a novel object, looked less at the owner when faced with an unsolvable problem, and performed worse on the short-term memory task. Previous test procedures for investigating age-related changes involve expensive and/or complicated devices and extensive pre-training. The main advantage of the proposed battery is to reduce costs and efforts in veterinary assessments. Further tests in same-breed, large samples and between dogs with mild and severe cognitive impairments will be needed in order to further validate the battery.
Do Behaviour Assessments in a Shelter Predict the Behaviour of Dogs Post-Adoption?
Authors: Clay Liam , Paterson Mandy B. A. , Bennett Pauleen , Perry Gaille , Phillips Clive C. J.
Published: July 2020

In shelters it is usual to conduct standardised behaviour assessments on admitted dogs. The information gathered from the assessment is used to identify dogs that are suitable for adoption and assist in matching the dog with suitable adopters. These assessments are also used to guide behaviour modification programs for dogs that display some unwanted behaviours. For some dogs, the results may indicate that they are unsuitable either for re-training or for adoption. In these circumstances the dogs may be euthanised. We investigated the predictive value of a standardised behaviour assessment protocol currently used in an Australian shelter for dog behaviour post-adoption. A total of 123 dogs, aged 1–10 years and housed in an animal care shelter, were assessed before they were adopted. The new owners of the dogs took part in a post-adoption survey conducted 1 month after adoption, which explored the behaviour of their dog after adoption. Ordinal regression analyses identified that friendly/social, fear and anxiousness identified in the shelter assessment significantly predicted corresponding behaviours post-adoption. However, behaviour problems, such as aggression, food guarding and separation-related behaviours, were not reliably predicted by the standardised behaviour assessment. The results suggest that further research is required to improve the predictability of behaviour assessment protocols for more specific behaviour problems, including different categories of aggression and separation-related problems. We recommend that dog behaviour assessments in shelters are used only in conjunction with other monitoring tools to assess behaviour over the whole shelter stay, thus facilitating increased safety/welfare standards for dogs, shelters and the wider community.
The Antioxidant Role of Soy and Soy Foods in Human Health
Authors: Rizzo Gianluca
Published: July 2020

Oxidative stress seems to play a role in many chronic diseases, such as cardiovascular diseases, diabetes, and some cancers. Research is always looking for effective approaches in the prevention and treatment of these pathologies with safe strategies. Given the central role of nutrition, the identification of beneficial healthy foods can be the best key to having a safe and at the same time effective approach. Soy has always aroused great scientific interest but often this attention is galvanized by the interaction with estrogen receptors and related consequences on health. However, soy, soy foods, and soy bioactive substances seem to have antioxidant properties, suggesting their role in quenching reactive oxygen species, although it was frequently mentioned but not studied in depth. The purpose of this review is to summarize the scientific evidence of the antioxidant properties of soy by identifying the human clinical trials available in the literature. A total of 58 manuscripts were individuated through the literature search for the final synthesis. Soy bioactive substances involved in redox processes appear to be multiple and their use seems promising. Other larger clinical trials with adequate standardization and adequate choice of biomarkers will fill the gap currently existing on the suggestive role of soy in antioxidant mechanisms.
Review on Mechanisms and Kinetics for Supercritical Water Oxidation Processes
Authors: Jiang Zhuohang , Li Yanhui , Wang Shuzhong , Cui Chengchao , Yang Chuang , Li Jianna
Published: July 2020

Supercritical water oxidation (SCWO) is a promising wastewater treatment technology owing to its various advantages such as rapid reactions and non-polluting products. However, problems like corrosion and salt decomposition set obstacles to its commercialization. To address these problems, researchers have been developing the optimal reactor design and strengthening measures based on sufficient understandings of the degradation kinetics. The essence of the SCWO process and the roles of oxygen and hydrogen peroxide are summarized in this work. Then, the research status and progress of empirical models, semi-empirical models, and detailed chemical kinetic models (DCKMs) are systematically reviewed. Additionally, this paper is the first to summarize the research progress of quantum chemistry and molecular dynamics simulation. The challenge and further development of kinetics models for the optimization of reactors and the directional transformation of pollutants are pointed out.
Image-Based Feature Representation for Insider Threat Classification
Authors: Gayathri R. G. , Sajjanhar Atul , Xiang Yong
Published: July 2020

Cybersecurity attacks can arise from internal and external sources. The attacks perpetrated by internal sources are also referred to as insider threats. These are a cause of serious concern to organizations because of the significant damage that can be inflicted by malicious insiders. In this paper, we propose an approach for insider threat classification which is motivated by the effectiveness of pre-trained deep convolutional neural networks (DCNNs) for image classification. In the proposed approach, we extract features from usage patterns of insiders and represent these features as images. Hence, images are used to represent the resource access patterns of the employees within an organization. After construction of images, we use pre-trained DCNNs for anomaly detection, with the aim to identify malicious insiders. Random under sampling is used for reducing the class imbalance issue. The proposed approach is evaluated using the MobileNetV2, VGG19, and ResNet50 pre-trained models, and a benchmark dataset. Experimental results show that the proposed method is effective and outperforms other state-of-the-art methods.
A Study of an Algorithm for the Surface Temperature Forecast: From Road Ice Risk to Farmland Application
Authors: Del Vecchio Maria Chiara , Ceppi Alessandro , Corbari Chiara , Ravazzani Giovanni , Mancini Marco , Spada Francesco , Maggioni Enrico , Perotto Alessandro , Salerno Raffaele
Published: July 2020

The presence of road ice has always been a key issue during winter months. A reliable forecast system capable of predicting the Land Surface Temperature (LST) and, consequently, its formation is one of the best strategies to operate towards reducing both vehicles accidents and waste of chemical solvents used for prevention which have a significant economic and environmental impact. Hence, the Meteo Expert Centre (MEC) has developed an algorithm for LST forecasts able to issue ice risk warnings as well. This algorithm operationally works every day in real-time and it is here tested, first, on a paved area of the Pedemontana Lombarda motorway and the Milano Linate airport airstrip, and, afterwards, since the LST plays a crucial role in understanding phenomena of energy exchange between soil, vegetation, and atmosphere, its knowledge and prediction becomes relevant also for other purposes such as agricultural management and irrigation system control, further experiments are carried out over two agricultural fields, one in the North and the other in the South of Italy during the SIM (Smart Irrigation Management) project. All LST analyses showed encouraging results with reasonable high values of statistical scores, in both applications on asphalted and different vegetated terrains, demonstrating that the developed algorithm has a high versatility even on completely different types of surfaces, and it can be applied as a valid tool for road ice risk warnings too.
Hydrogels Obtained via γ-Irradiation Based on Poly(Acrylic Acid) and Its Copolymers with 2-Hydroxyethyl Methacrylate
Authors: Micutz Marin , Lungu Rodica Mihaela , Circu Viorel , Ilis Monica , Staicu Teodora
Published: July 2020

Hydrogels containing both carboxyl and hydroxyl functional groups have been prepared by γ-irradiation of either aqueous solutions of acrylic acid (AA) and mixtures of AA and 2-hydroxyethyl methacrylate (HEMA) in different ratios, or aqueous solutions of poly(AA), PAA, and poly(AA-co-HEMA) obtained via solution polymerization. A higher absorbed dose is required in order to prepare hydrogels from monomer solutions, compared with those from polymer solutions. The range for the absorbed doses was chosen so that the probability of crosslinking reactions is higher than that of degradation ones. As the radiation energy deposited in a sample increases, the equilibrium swelling degree and the average molar mass between crosslinks diminishes. Chemical transformations induced by radiation were investigated by means of FTIR spectroscopy and thermal analysis of polymers before and after irradiation. For all these systems, the formation of a three-dimensional network enhances the glass transition temperature and thermal stability, but a further increase in the crosslinking degree may have the reverse effect on the glass transition temperature. Depending on the preparation protocol and/or hydrogel composition, superabsorbent materials that can bind different compounds throughout side functional groups may be obtained.
Data-Driven Knowledge-Based System for Self-Measuring Activities of Daily Living in IoT-Based Test
Authors: Shin Youngsul , Park Yu Jin , Kang Soon Ju
Published: July 2020

This paper proposes a data-driven knowledge-based system with which aged people can measure the degree of activities of daily living (ADL) by themselves. The proposed system, called E-coach for ADL Test (EAT), provides participants with self-measurement procedures, using e-coaching, which is a guidance mechanism to lead the participants from an initial stage to a target goal. The EAT traces the behavior of the participants to gather ADL data that tell how well they perform the given e-coaching. Driven by the Internet of Things data, the knowledge-based inference of the EAT carries out the e-coaching mechanism that figures out what state the self-measurement procedures stay on and what guidance is necessary for the next state. The EAT ensures that all the procedures for ADL measurement are executed automatically without any help from medical professionals. The experiment described in this paper demonstrates that the EAT distinguishes between dementia patients and normal people. The measurement report assists medical doctors in the diagnosis of certain medical conditions that these people may have.
Development of Direct-Vibration Actuator for Bezel-Less Display Panels on Mobile Phones
Authors: Park Ki-Hong , Jiang Zhi-Xiong , Jiang Yuan-Wu , Hwang Sang-Moon
Published: July 2020

With the development of technology, multimedia devices such as smartphones, tablets, and wearable devices have become necessities in our lives, and many new products are introduced every year. Companies are expanding smartphone displays and are developing bezel-less display panel designs. The enlarged display limits the space available for a speaker, and a new actuator must therefore be developed. Indirect-vibration actuators were developed for full-wide display designs. Using the same sound-generation principle as that of the indirect-vibration actuator, the mechanism and design of the direct-vibrating actuator is proposed in this paper. Using 3D finite element method (FEM), the force factor is obtained and used for design optimization. A sample is produced, and an experiment is conducted for sound pressure level (SPL) comparison. The experiment results show that the newly designed direct-vibration actuator can replace the dynamic receiver in mobile devices and enable the application of the bezel-less display design.
An Inspection Robot for Belt Conveyor Maintenance in Underground Mine—Infrared Thermography for Overheated Idlers Detection
Authors: Szrek Jarosław , Wodecki Jacek , Błażej Ryszard , Zimroz Radoslaw
Published: July 2020

It is well known that mechanical systems require supervision and maintenance procedures. There are a lot of condition monitoring techniques that are commonly used, and in the era of IoT and predictive maintenance one may find plenty of solutions for various applications. Unfortunately in the case of belt conveyors used in underground mining a list of possible solutions shrinks quickly. The reason is that they are specific mechanical systems—the typical conveyor is located in the mining tunnel and its length may vary between 100 and 1000 m. According to mining regulations, visual inspection of the conveyor route should be done before it will start the operation. On the other hand, since environmental conditions in mining tunnels are extremely harsh and the risk of accidents is high, there is a tendency to minimize human presence in the tunnels. In this paper, we propose a prototype of an inspection robot based on a UGV platform that could support maintenance staff during the inspection. At present, the robot is controlled by an operator using radio however, we plan to make it autonomous. Moreover, its support could be significant—the robot can “see” elements of the conveyor route (RGB camera) and can identify hot spots using infrared thermography. Moreover, the detected hot spots could be localized and its position can be stored together with both types of images. In parallel, it is possible to preview images in a real-time and stored data allow analysing state of conveyor system after the inspection mission. It is also important that due to radio control systems, an operator can stay in a safe place. Such a robot can be classified as a mobile monitoring system for spatially distributed underground infrastructure.
Thermophilic Methane Production from Hydrothermally Pretreated Norway Spruce (Picea abies)
Authors: Ghimire Nirmal , Bakke Rune , Bergland Wenche Hennie
Published: July 2020

Norway spruce (Picea abies) is an industrially important softwood species available in northern Europe and can be used to produce bio-methane after proper pretreatment to overcome its recalcitrant complex structure. Hot water extraction (HWE) pretreatment at two different conditions (170 °C for 90 min (severity 4.02) and 140 °C for 300 min (severity 3.65)) was applied to extract hemicellulosic sugars from Norway spruce for thermophilic anaerobic digestion (AD) of the hydrolysate. The methane yield of hydrolysate prepared at the lower pretreatment severity was found to be 189 NmL/gCOD compared to 162 NmL/gCOD after the higher pretreatment severity suggesting higher pretreatment severity hampers the methane yield due to the presence of inhibitors formed due to sugars and lignin degradation and soluble lignin, extracted partially along with hemicellulosic sugars. Synthetic hydrolysates simulating real hydrolysates (H170syn and H140syn) had improved methane yield of 285 NmL/gCOD and 295 NmL/gCOD, respectively in the absence of both the inhibitors and soluble lignin. An effect of organic loadings (OLs) on the methane yield was observed with a negative correlation between OL and methane yield. The maximum methane yield was 290 NmL/gCOD for hydrolysate pretreated at 140 °C compared to 195 NmL/gCOD for hydrolyate pretreated at 170 °C, both at the lowest OL of 6 gCOD/L. Therefore, both pretreatment conditions and OL need to be considered for efficient methane production from extracted hydrolysate. Such substrates can be utilized in continuous flow industrial AD with well-adapted cultures with stable organic loading rates.
Road to Fame: Social Trajectory of Takahata Isao
Authors: Yoshioka Shiro
Published: July 2020

This paper examines how Takahata Isao’s reputation as a filmmaker was established, focusing on the period between Horus: The Prince of the Sun (1968) and Only Yesterday (1991), using Pierre Bourdieu’s concepts of “field” and “consecration”. Through detailed analysis of promotion strategies, popular and critical reception of his films, and his appearance in different types of media in the form of essays and interviews, I will discuss how Takahata and his films were “consecrated”, or came to be recognized as something respectable and deserve critical attention. Throughout the analysis the focus will be on the relationship between different “fields” rather than his films. I will contend that the process of his consecration is deeply related to that of the establishment of the field of anime and its fandom in the late 1970s, and its relationship with other fields with greater cultural capital, such as literature and live-action films as well as non-Japanese animations. The association of Takahata and his films with these fields was used by media, stakeholders in film productions including Studio Ghibli and publishing houses Tokuma shoten and Shinchōsha, as well as Takahata himself, to distinguish him and his films from other anime.
Impacts of Vegetation and Topography on Land Surface Temperature Variability over the Semi-Arid Mountain Cities of Saudi Arabia
Authors: Bindajam Ahmed Ali , Mallick Javed , AlQadhi Saeed , Singh Chander Kumar , Hang Hoang Thi
Published: July 2020

Land surface temperature (LST) can fully reflect the water–heat exchange cycle of the earth surface that is important for the study of environmental change. There is little research on LST in the semi-arid region of Abha-Khamis-Mushyet, which has a complex topography. The study used LST data, retrieved from ASTER data in semi-arid mountain areas and discussed its relationship with land use/land cover (LULC), topography and the normalized difference vegetation index (NDVI). The results showed that the LST was significantly influenced by altitude and corresponding LULC type. In the study area, during the summer season, extreme high-temperature zones were observed, possibly due to dense concrete surfaces. LST among different types of land use differed significantly, being the highest in exposed rocky areas and built-up land, and the lowest in dense vegetation. NDVI and LST spatial distributions showed opposite trends. The LST–NDVI feature space showed a unique ABC obtuse-angled triangle shape and showed an overall negative linear correlation. In brief, the LST could be retrieved well by the emissivity derived NDVI TES method, which relied on upwelling, downwelling, and transmittance. In addition, the LST of the semi-arid mountain areas was influenced by elevation, slope zenith angle, aspect and LULC, among which vegetation and elevation played a key role in the overall LST. This research provides a roadmap for land-use planning and environmental conservation in mountainous urban areas.
Analysis of Pollution Characteristics and Influencing Factors of Main Pollutants in the Atmosphere of Shenyang City
Authors: Tian Jiaqi , Fang Chunsheng , Qiu Jiaxin , Wang Ju
Published: July 2020

Air pollution is one of the most concerning environmental problems in cities. Hourly data on pollutant concentrations from 11 automatic atmospheric monitoring stations and meteorological data in Shenyang from 2017 to 2019 were used to analyze the spatio-temporal variation rules of CO (carbon monoxide), SO2 (sulfur dioxide), NO2 (nitrogen dioxide), O3 (ozone), PM2.5 and PM10 (PM particles with an aerodynamic diameters of not more than 2.5 µm and 10 µm) and their relationships with meteorological parameters. Meanwhile, the regional transmission route of pollutants was analyzed by the hybrid single particle Lagrangian integrated trajectory (HYSPLIT) model. The results showed that the concentration of O3 in the northern area of the city was higher than that in the south; CO, SO2 and NO2 were relatively high in the urban center; and PM2.5 and PM10 were relatively high in the southwest. The average concentration of pollutants was lowest in 2019. The concentration of O3 was the highest in spring, while CO showed no significant variations between different seasons. The remaining pollutant concentrations appeared to be high in winter and low in summer. The cumulative concentrations of the six pollutants were the highest in March, and relatively low in July–September. The diurnal concentration variations of O3, CO and SO2 exhibited a “single peak,” while others showed a “double peak and double valley.” Temperature was positively correlated with O3 concentration and negatively correlated with others. Wind speed was negatively correlated with the concentration of PM2.5, NO2, and O3. The air quality of the main urban area in spring and summer was mainly affected by the coastal air flow, while it was mostly affected by the northwest air flow in autumn and winter.
Human Embryonic-Derived Mesenchymal Progenitor Cells (hES-MP Cells) are Fully Supported in Culture with Human Platelet Lysates
Authors: Jonsdottir-Buch Sandra M. , Gunnarsdottir Kristbjorg , Sigurjonsson Olafur E.
Published: July 2020

Human embryonic stem cell-derived mesenchymal progenitor (hES-MP) cells are mesenchymal-like cells, derived from human embryonic stem cells without the aid of feeder cells. They have been suggested as a potential alternative to mesenchymal stromal cells (MSCs) in regenerative medicine due to their mesenchymal-like proliferation and differentiation characteristics. Cells and cell products intended for regenerative medicine in humans should be derived, expanded and differentiated using conditions free of animal-derived products to minimize risk of animal-transmitted disease and immune reactions to foreign proteins. Human platelets are rich in growth factors needed for cell culture and have been used successfully as an animal serum replacement for MSC expansion and differentiation. In this study, we compared the proliferation of hES-MP cells and MSCs; the hES-MP cell growth was sustained for longer than that of MSCs. Growth factors, gene expression, and surface marker expression in hES-MP cells cultured with either human platelet lysate (hPL) or fetal bovine serum (FBS) supplementation were compared, along with differentiation to osteogenic and chondrogenic lineages. Despite some differences between hES-MP cells grown in hPL- and FBS-supplemented media, hPL was found to be a suitable replacement for FBS. In this paper, we demonstrate for the first time that hES-MP cells can be grown using platelet lysates from expired platelet concentrates (hPL).
Sperm Quality Assessment in Honey Bee Drones
Authors: Yániz Jesús L. , Silvestre Miguel A. , Santolaria Pilar
Published: July 2020

The quality of honey bee drone semen is relevant in different contexts, ranging from colony productivity to pathology, toxicology and biodiversity preservation. Despite its importance, considerably less knowledge is available on this subject for the honey bee when compared to other domestic animal species. A proper assessment of sperm quality requires a multiple testing approach which discriminates between the different aspects of sperm integrity and functionality. Most studies on drone semen quality have only assessed a few parameters, such as sperm volume, sperm concentration and/or sperm plasma membrane integrity. Although more recent studies have focused on a broader variety of aspects of semen quality, some techniques currently used in vertebrates, such as computer-assisted sperm analysis (CASA) or multiparametric sperm quality testing, still remain to be developed in the honey bee. This may be attributed to the particular sperm morphology and physiology in this species, requiring the development of technologies specifically adapted to it. This article reviews the present knowledge of sperm quality in honey bee drones, highlighting its peculiarities and proposing future lines of research.
ProAKAP4 as Novel Molecular Marker of Sperm Quality in Ram: An Integrative Study in Fresh, Cooled and Cryopreserved Sperm
Authors: F. Riesco Marta , Anel-Lopez Luis , Neila-Montero Marta , Palacin-Martinez Cristina , Montes-Garrido Rafael , Alvarez Mercedes , de Paz Paulino , Anel Luis
Published: July 2020

To improve artificial insemination protocols in ovine species it is crucial to optimize sperm quality evaluation after preservation technologies. Emerging technologies based on novel biomolecules and related to redox balance and proteins involved in sperm motility such as ProAKAP4 could be successfully applied in ram sperm evaluation. In this work, a multiparametric analysis of fresh, cooled, and cryopreserved ram sperm was performed at different complexity levels. Samples were evaluated in terms of motility (total motility, progressive motility, and curvilinear velocity), viability, apoptosis, content of reactive oxygen species, oxidation‒reduction potential, and ProAKAP4 expression and concentration. As expected, cryopreserved samples showed a significant decrease of sperm quality (p < 0.05), evidencing different freezability classes among samples that were detected by ProAKAP4 analyses. However, in cooled sperm no differences were found concerning motility, viability, apoptosis, ROS content, and redox balance compared to fresh sperm that could explain the reported decrease in fertility rates. However, although the proportion of sperm ProAKAP4 positive-cells remained unaltered in cooled sperm compared to fresh control, the concentration of this protein significantly decreased (p < 0.05) in cooled samples. This altered protein level could contribute to the decrease in fertility rates of cooled samples detected by some authors. More importantly, ProAKAP4 can be established as a promising diagnostic parameter of sperm quality allowing us to optimize sperm conservation protocols and finally improve artificial insemination in ovine species.
Deamidated Human Triosephosphate Isomerase is a Promising Druggable Target
Authors: Enríquez-Flores Sergio , Flores-López Luis Antonio , García-Torres Itzhel , de la Mora-de la Mora Ignacio , Cabrera Nallely , Gutiérrez-Castrellón Pedro , Martínez-Pérez Yoalli , López-Velázquez Gabriel
Published: July 2020

Therapeutic strategies for the treatment of any severe disease are based on the discovery and validation of druggable targets. The human genome encodes only 600–1500 targets for small-molecule drugs, but posttranslational modifications lead to a considerably larger druggable proteome. The spontaneous conversion of asparagine (Asn) residues to aspartic acid or isoaspartic acid is a frequent modification in proteins as part of the process called deamidation. Triosephosphate isomerase (TIM) is a glycolytic enzyme whose deamidation has been thoroughly studied, but the prospects of exploiting this phenomenon for drug design remain poorly understood. The purpose of this study is to demonstrate the properties of deamidated human TIM (HsTIM) as a selective molecular target. Using in silico prediction, in vitro analyses, and a bacterial model lacking the tim gene, this study analyzed the structural and functional differences between deamidated and nondeamidated HsTIM, which account for the efficacy of this protein as a druggable target. The highly increased permeability and loss of noncovalent interactions of deamidated TIM were found to play a central role in the process of selective enzyme inactivation and methylglyoxal production. This study elucidates the properties of deamidated HsTIM regarding its selective inhibition by thiol-reactive drugs and how these drugs can contribute to the development of cell-specific therapeutic strategies for a variety of diseases, such as COVID-19 and cancer.
Vitamin D Prevents Pancreatic Cancer-Induced Apoptosis Signaling of Inflammatory Cells
Authors: Moz Stefania , Contran Nicole , Facco Monica , Trimarco Valentina , Plebani Mario , Basso Daniela
Published: July 2020

Combined approaches based on immunotherapy and drugs supporting immune effector cell function might increase treatment options for pancreatic ductal adenocarcinoma (PDAC), vitamin D being a suitable drug candidate. In this study, we evaluated whether treatment with the vitamin D analogue, calcipotriol, counterbalances PDAC induced and SMAD4-associated intracellular calcium [Ca2+]i alterations, cytokines release, immune effector function, and the intracellular signaling of peripheral blood mononuclear cells (PBMCs). Calcipotriol counteracted the [Ca2+]i depletion of PBMCs induced by SMAD4-expressing PDAC cells, which conditioned media augmented the number of calcium flows while reducing whole [Ca2+]i. While calcipotriol inhibited spontaneous and PDAC-induced tumor necrosis factor alpha (TNF-α) release by PBMC and reduced intracellular transforming growth factor beta (TGF-β), it did not counteract the lymphocytes proliferation induced in allogenic co-culture by PDAC-conditioned PBMCs. Calcipotriol mainly antagonized PDAC-induced apoptosis and partially restored PDAC-inhibited NF-κB signaling pathway. In conclusion, alterations induced by PDAC cells in the [Ca2+]i of immune cells can be partially reverted by calcipotriol treatment, which promotes inflammation and antagonizes PBMCs apoptosis. These effects, together with the dampening of intracellular TGF-β, might result in an overall anti-tumor effect, thus supporting the administration of vitamin D in PDAC patients.
Epigenetic Mechanisms of Resistance to Immune Checkpoint Inhibitors
Authors: Perrier Alexandre , Didelot Audrey , Laurent-Puig Pierre , Blons Hélène , Garinet Simon
Published: July 2020

Immune checkpoint inhibitors (ICIs) have demonstrated to be highly efficient in treating solid tumors; however, many patients have limited benefits in terms of response and survival. This rapidly led to the investigation of combination therapies to enhance response rates. Moreover, predictive biomarkers were assessed to better select patients. Although PD-L1 expression remains the only validated marker in clinics, molecular profiling has brought valuable information, showing that the tumor mutation load and microsatellite instability (MSI) status were associated to higher response rates in nearly all cancer types. Moreover, in lung cancer, EGFR and MET mutations, oncogene fusions or STK11 inactivating mutations were associated with low response rates. Cancer progression towards invasive phenotypes that impede immune surveillance relies on complex regulatory networks and cell interactions within the tumor microenvironment. Epigenetic modifications, such as the alteration of histone patterns, chromatin structure, DNA methylation status at specific promoters and changes in microRNA levels, may alter the cell phenotype and reshape the tumor microenvironment, allowing cells to grow and escape from immune surveillance. The objective of this review is to make an update on the identified epigenetic changes that target immune surveillance and, ultimately, ICI responses, such as histone marks, DNA methylation and miR signatures. Translational studies or clinical trials, when available, and potential epigenetic biomarkers will be discussed as perspectives in the context of combination treatment strategies to enhance ICI responses in patients with solid tumors.
Understanding the Biosynthetic Changes that Give Origin to the Distinctive Flavor of Sotol: Microbial Identification and Analysis of the Volatile Metabolites Profiles During Sotol (Dasylirion sp.) Must Fermentation
Authors: Zavala-Díaz de la Serna Francisco Javier , Contreras-López Ricardo , Lerma-Torres L. Paola , Ruiz-Terán Francisco , Rocha-Gutiérrez Beatriz A. , Pérez-Vega Samuel B. , Elías-Ogaz Leslie R. , Salmerón Ivan
Published: July 2020

In northern Mexico, the distilled spirit sotol with a denomination of origin is made from species of Dasylirion. The configuration of the volatile metabolites produced during the spontaneous fermentation of Dasylirion sp. must is insufficiently understood. In this study, the aim was to investigate the composition of the microbial consortia, describe the variation of volatile metabolites, and relate such profiles with their particular flavor attributes during the fermentation of sotol (Dasylirion sp.) must. Ascomycota was the phylum of most strains identified with 75% of total abundance. The genus of fermenting yeasts constituted of 101 Pichia strains and 13 Saccharomyces strains. A total of 57 volatile metabolites were identified and grouped into ten classes. The first stage of fermentation was composed of diesel, green, fruity, and cheesy attributes due to butyl 2-methylpropanoate, octan-1-ol, ethyl octanoate, and butanal, respectively, followed by a variation to pungent and sweet descriptors due to 3-methylbutan-1-ol and butyl 2-methylpropanoate. The final stage was described by floral, ethereal-winey, and vinegar attributes related to ethyl ethanimidate, 2-methylpropan-1-ol, and 2-hydroxyacetic acid. Our results improve the knowledge of the variations of volatile metabolites during the fermentation of sotol must and their contribution to its distinctive flavor.
The Long-Acting D3 Partial Agonist MC-25-41 Attenuates Motivation for Cocaine in Sprague-Dawley Rats
Authors: Powell Gregory L. , Namba Mark D. , Vannan Annika , Bonadonna John Paul , Carlson Andrew , Mendoza Rachel , Chen Peng-Jen , Luetdke Robert R. , Blass Benjamin E. , Neisewander Janet L.
Published: July 2020

The dopamine D3 receptor is a prime target for developing treatments for cocaine use disorders (CUDs). In this study, we conducted a pre-clinical investigation of the therapeutic potential of a long-acting, D3 receptor partial agonist, MC-25-41. Male rats were pre-treated with MC-25-41 (vehicle, 1.0, 3.0, 5.6, or 10 mg/kg, intraperitoneal (IP)) five minutes prior to tests of cocaine or sucrose intake on either a progressive ratio schedule of reinforcement or a variable interval 60 s multiple schedule consisting of 4, 15-min components with sucrose or cocaine available in alternating components. A separate cohort of rats was tested on a within-session, dose-reduction procedure to determine the effects of MC-25-41 on demand for cocaine using a behavioral economics analysis. Finally, rats were tested for effects of MC-25-41 on spontaneous and cocaine-induced locomotion. MC-25-41 failed to alter locomotion, but reduced reinforcement rates for both cocaine and sucrose on the low-effort, multiple schedule. However, on the higher-effort, progressive ratio schedule of cocaine reinforcement, MC-25-41 reduced infusions, and active lever presses at doses that did not alter sucrose intake. The behavioral economics analysis showed that MC-25-41 also increased cocaine demand elasticity compared to vehicle, indicating a reduction in consumption as price increases. Together, these results suggest that similar to other D3-selective antagonists and partial agonists, MC-25-41 reduces motivation for cocaine under conditions of high cost but has the added advantage of a long half-life (>10 h). These findings suggest that MC-25-41 may be a suitable pre-clinical lead compound for development of medications to treat CUDs.
Coupling Ion Specificity of the Flagellar Stator Proteins MotA1/MotB1 of Paenibacillus sp. TCA20
Authors: Onoe Sakura , Yoshida Myu , Terahara Naoya , Sowa Yoshiyuki
Published: July 2020

The bacterial flagellar motor is a reversible rotary molecular nanomachine, which couples ion flux across the cytoplasmic membrane to torque generation. It comprises a rotor and multiple stator complexes, and each stator complex functions as an ion channel and determines the ion specificity of the motor. Although coupling ions for the motor rotation were presumed to be only monovalent cations, such as H+ and Na+, the stator complex MotA1/MotB1 of Paenibacillus sp. TCA20 (MotA1TCA/MotB1TCA) was reported to use divalent cations as coupling ions, such as Ca2+ and Mg2+. In this study, we initially aimed to measure the motor torque generated by MotA1TCA/MotB1TCA under the control of divalent cation motive force; however, we identified that the coupling ion of MotA1TCAMotB1TCA is very likely to be a monovalent ion. We engineered a series of functional chimeric stator proteins between MotB1TCA and Escherichia coli MotB. E. coli ΔmotAB cells expressing MotA1TCA and the chimeric MotB presented significant motility in the absence of divalent cations. Moreover, we confirmed that MotA1TCA/MotB1TCA in Bacillus subtilis ΔmotABΔmotPS cells generates torque without divalent cations. Based on two independent experimental results, we conclude that the MotA1TCA/MotB1TCA complex directly converts the energy released from monovalent cation flux to motor rotation.
Novel MRI Techniques Identifying Vascular Leak and Paravascular Flow Reduction in Early Alzheimer Disease
Authors: Joseph Charles R
Published: July 2020

With beta amyloid and tau antibody treatment trial failures, avenues directed to other facets of the disease pathophysiology are being explored to treat in the preclinical or early clinical state. Clear evidence of blood–brain barrier (BBB) breakdown occurring early in the AD process has recently been established. Likewise, the glymphatic system regulating water and solute inflow and outflow in parallel with the vascular system is affected causing delayed clearance of fluid waste. Its dysfunction as a component of AD along with BBB leak are reasonable candidates to explore for future treatments. Ideally, human medication trials require a minimally invasive method of quantifying both improvements in BBB integrity and glymphatic fluid clearance correlated with clinical outcomes. We will review the known physiology and anatomy of the BBB system, and its relationship to the glymphatic system and the microglial surveillance system. Dysfunction of this tripart system occurring in preclinical Alzheimer disease (AD) will be reviewed along with existing MRI tools for identifying altered flow dynamics useful for monitoring improved functionality with future treatments. High-resolution dynamic contrast enhanced MRI imaging demonstrating BBB leak and the recently reported non-invasive 3D PASL MRI pilot study demonstrating significant delay in glymphatic clearance in AD subjects appear to be the best candidates.
The Disengagement of Visual Attention: An Eye-Tracking Study of Cognitive Impairment, Ethnicity and Age
Authors: Polden Megan , Wilcockson Thomas D. W. , Crawford Trevor J.
Published: July 2020

Various studies have shown that Alzheimer’s disease (AD) is associated with an impairment of inhibitory control, although we do not have a comprehensive understanding of the associated cognitive processes. The ability to engage and disengage attention is a crucial cognitive operation of inhibitory control and can be readily investigated using the “gap effect” in a saccadic eye movement paradigm. In previous work, various demographic factors were confounded; therefore, here, we examine separately the effects of cognitive impairment in Alzheimer’s disease, ethnicity/culture and age. This study included young (N = 44) and old (N = 96) European participants, AD (N = 32), mildly cognitively impaired participants (MCI: N = 47) and South Asian older adults (N = 94). A clear reduction in the mean reaction times was detected in all the participant groups in the gap condition compared to the overlap condition, confirming the effect. Importantly, this effect was also preserved in participants with MCI and AD. A strong effect of age was also evident, revealing a slowing in the disengagement of attention during the natural process of ageing.
COVID-19: Pain Management in Patients with SARS-CoV-2 Infection—Molecular Mechanisms, Challenges, and Perspectives
Authors: Drożdżal Sylwester , Rosik Jakub , Lechowicz Kacper , Machaj Filip , Szostak Bartosz , Majewski Paweł , Rotter Iwona , Kotfis Katarzyna
Published: July 2020

Since the end of 2019, the whole world has been struggling with the pandemic of the new Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV-2). Available evidence suggests that pain is a common symptom during Coronavirus Disease 2019 (COVID-19). According to the World Health Organization, many patients suffer from muscle pain (myalgia) and/or joint pain (arthralgia), sore throat and headache. The exact mechanisms of headache and myalgia during viral infection are still unknown. Moreover, many patients with respiratory failure get admitted to the intensive care unit (ICU) for ventilatory support. Pain in ICU patients can be associated with viral disease itself (myalgia, arthralgia, peripheral neuropathies), may be caused by continuous pain and discomfort associated with ICU treatment, intermittent procedural pain and chronic pain present before admission to the ICU. Undertreatment of pain, especially when sedation and neuromuscular blocking agents are used, prone positioning during mechanical ventilation or extracorporeal membrane oxygenation (ECMO) may trigger delirium and cause peripheral neuropathies. This narrative review summarizes current knowledge regarding challenges associated with pain assessment and management in COVID-19 patients. A structured prospective evaluation should be undertaken to analyze the probability, severity, sources and adequate treatment of pain in patients with COVID-19 infection.