bluuebunny/crossref_metadata_2025 huggingface dataset:
The Potential Benefits of Quercetin for Brain Health: A Review of Anti-Inflammatory and Neuroprotective Mechanisms
Authors: Chiang Ming-Chang , Tsai Tsung-Yu , Wang Chieh-Ju
Published: March 2023

Neuroinflammation is a critical factor in developing and progressing numerous brain diseases, including neurodegenerative diseases. Chronic or excessive neuroinflammation can lead to neurotoxicity, causing brain damage and contributing to the onset and progression of various brain diseases. Therefore, understanding neuroinflammation mechanisms and developing strategies to control them is crucial for treating brain diseases. Studies have shown that neuroinflammation plays a vital role in the progression of neurodegenerative diseases, such as Alzheimer’s (AD) and Parkinson’s (PD), and stroke. Additionally, the effects of PM2.5 pollution on the brain, including neuroinflammation and neurotoxicity, are well-documented. Quercetin is a flavonoid, a plant pigment in many fruits, vegetables, and grains. Quercetin has been studied for its potential health benefits, including its anti-inflammatory, antioxidant, and anti-cancer properties. Quercetin may also have a positive impact on immune function and allergy symptoms. In addition, quercetin has been shown to have anti-inflammatory and neuroprotective properties and can activate AMP-activated protein kinase (AMPK), a cellular energy sensor that modulates inflammation and oxidative stress. By reducing inflammation and protecting against neuroinflammatory toxicity, quercetin holds promise as a safe and effective adjunctive therapy for treating neurodegenerative diseases and other brain disorders. Understanding and controlling the mechanisms of NF-κB and NLRP3 inflammasome pathways are crucial for preventing and treating conditions, and quercetin may be a promising tool in this effort. This review article aims to discuss the role of neuroinflammation in the development and progression of various brain disorders, including neurodegenerative diseases and stroke, and the impact of PM2.5 pollution on the brain. The paper also highlights quercetin’s potential health benefits and anti-inflammatory and neuroprotective properties.
Role of Macrophages in Sickle Cell Disease Erythrophagocytosis and Erythropoiesis
Authors: Sesti-Costa Renata , Costa Fernando F. , Conran Nicola
Published: March 2023

Sickle cell disease (SCD) is an inherited blood disorder caused by a β-globin gene point mutation that results in the production of sickle hemoglobin that polymerizes upon deoxygenation, causing the sickling of red blood cells (RBCs). RBC deformation initiates a sequence of events leading to multiple complications, such as hemolytic anemia, vaso-occlusion, chronic inflammation, and tissue damage. Macrophages participate in extravascular hemolysis by removing damaged RBCs, hence preventing the release of free hemoglobin and heme, and triggering inflammation. Upon erythrophagocytosis, macrophages metabolize RBC-derived hemoglobin, activating mechanisms responsible for recycling iron, which is then used for the generation of new RBCs to try to compensate for anemia. In the bone marrow, macrophages can create specialized niches, known as erythroblastic islands (EBIs), which regulate erythropoiesis. Anemia and inflammation present in SCD may trigger mechanisms of stress erythropoiesis, intensifying RBC generation by expanding the number of EBIs in the bone marrow and creating new ones in extramedullary sites. In the current review, we discuss the distinct mechanisms that could induce stress erythropoiesis in SCD, potentially shifting the macrophage phenotype to an inflammatory profile, and changing their supporting role necessary for the proliferation and differentiation of erythroid cells in the disease. The knowledge of the soluble factors, cell surface and intracellular molecules expressed by EBI macrophages that contribute to begin and end the RBC’s lifespan, as well as the understanding of their signaling pathways in SCD, may reveal potential targets to control the pathophysiology of the disease.
Specialized Ribosomes in Health and Disease
Authors: Miller Sarah C. , MacDonald Clinton C. , Kellogg Morgana K. , Karamysheva Zemfira N. , Karamyshev Andrey L.
Published: March 2023

Ribosomal heterogeneity exists within cells and between different cell types, at specific developmental stages, and occurs in response to environmental stimuli. Mounting evidence supports the existence of specialized ribosomes, or specific changes to the ribosome that regulate the translation of a specific group of transcripts. These alterations have been shown to affect the affinity of ribosomes for certain mRNAs or change the cotranslational folding of nascent polypeptides at the exit tunnel. The identification of specialized ribosomes requires evidence of the incorporation of different ribosomal proteins or of modifications to rRNA and/or protein that lead(s) to physiologically relevant changes in translation. In this review, we summarize ribosomal heterogeneity and specialization in mammals and discuss their relevance to several human diseases.
Neutrophils in Health and Disease: From Receptor Sensing to Inflammasome Activation
Authors: Iwaniuk Agnieszka , Jablonska Ewa
Published: March 2023

Neutrophils—polymorphonuclear cells (PMNs) are the cells of the initial immune response and make up the majority of leukocytes in the peripheral blood. After activation, these cells modify their functional status to meet the needs at the site of action or according to the agent causing injury. They receive signals from their surroundings and “plan” the course of the response in both temporal and spatial contexts. PMNs dispose of intracellular signaling pathways that allow them to perform a wide range of functions associated with the development of inflammatory processes. In addition to these cells, some protein complexes, known as inflammasomes, also have a special role in the development and maintenance of inflammation. These complexes participate in the proteolytic activation of key pro-inflammatory cytokines, such as IL-1β and IL-18. In recent years, there has been significant progress in the understanding of the structure and molecular mechanisms behind the activation of inflammasomes and their participation in the pathogenesis of numerous diseases. The available reports focus primarily on macrophages and dendritic cells. According to the literature, the activation of inflammasomes in neutrophils and the associated death type—pyroptosis—is regulated in a different manner than in other cells. The present work is a review of the latest reports concerning the course of inflammasome activation and inflammatory cytokine secretion in response to pathogens in neutrophils, as well as the role of these mechanisms in the pathogenesis of selected diseases.
Cytokine Signatures in Psoriatic Arthritis Patients Indicate Different Phenotypic Traits Comparing Responders and Non-Responders of IL-17A and TNFα Inhibitors
Authors: Skougaard Marie , Ditlev Sisse Bolm , Søndergaard Magnus Friis , Kristensen Lars Erik
Published: March 2023

This study aimed to explore the dynamic interactions between 32 cytokines and biomarkers in Psoriatic Arthritis (PsA) patients to compare cytokine signatures of treatment responders and non-responders. Biomarkers were measured before and after four months of treatment in 39 PsA patients initiating either Tumor Necrosis Factor alpha inhibitor (TNFi) or Interleukin-17A inhibitor (IL-17Ai). Response to treatment was defined by the composite measure, Disease Activity in Psoriatic Arthritis (DAPSA). A two-component principal component analysis (PCA) was implemented to describe cytokine signatures comparing DAPSA50 responders and non-responders. The cytokine signature of TNFi responders was driven by the correlated cytokines interferon γ (IFNγ) and IL-6, additionally associated with IL-12/IL-23p40, TNFα, and CRP, while the cytokine signature of TNFi non-responders was driven by the correlated cytokines IL-15, IL-8, and IFNγ. IL-17Ai responders were characterized by contributions of strongly correlated Th17 inflammatory cytokines, IL-17A, IL-12/IL-23p40, IL-22 to the cytokine signature, whereas IL-17A and IL-12/IL-23p40 did not demonstrate significant contribution in IL-17Ai non-responders. Based on PCA results it was possible to differentiate DAPSA50 responders and non-responders to treatment, endorsing additional examination of cytokine interaction models in PsA patients and supporting further PsA patient immune stratification to improve individualized treatment of PsA patients.
Water-Soluble Polymer Polyethylene Glycol: Effect on the Bioluminescent Reaction of the Marine Coelenterate Obelia and Coelenteramide-Containing Fluorescent Protein
Authors: Siniakova Tatiana S. , Raikov Alexander V. , Kudryasheva Nadezhda S.
Published: March 2023

The current paper considers the effects of a water-soluble polymer (polyethylene glycol (PEG)) on the bioluminescent reaction of the photoprotein obelin from the marine coelenterate Obelia longissima and the product of this bioluminescent reaction: a coelenteramide-containing fluorescent protein (CCFP). We varied PEG concentrations (0–1.44 mg/mL) and molecular weights (1000, 8000, and 35,000 a.u.). The presence of PEG significantly increased the bioluminescent intensity of obelin but decreased the photoluminescence intensity of CCFP; the effects did not depend on the PEG concentration or the molecular weight. The photoluminescence spectra of CCFP did not change, while the bioluminescence spectra changed in the course of the bioluminescent reaction. The changes can be explained by different rigidity of the media in the polymer solutions affecting the stability of the photoprotein complex and the efficiency of the proton transfer in the bioluminescent reaction. The results predict and explain the change in the luminescence intensity and color of the marine coelenterates in the presence of water-soluble polymers. The CCFP appeared to be a proper tool for the toxicity monitoring of water-soluble polymers (e.g., PEGs).
Aging-Related Mechanisms Contribute to Corticosteroid Insensitivity in Elderly Asthma
Authors: Ford Maria L. , Ruwanpathirana Anushka , Lewis Brandon W. , Britt Rodney D.
Published: March 2023

Asthma in elderly populations is an increasing health problem that is accompanied by diminished lung function and frequent exacerbations. As potent anti-inflammatory drugs, corticosteroids are commonly used to reduce lung inflammation, improve lung function, and manage disease symptoms in asthma. Although effective for most individuals, older patients are more insensitive to corticosteroids, making it difficult to manage asthma in this population. With the number of individuals older than 65 continuing to increase, it is important to understand the distinct mechanisms that promote corticosteroid insensitivity in the aging lung. In this review, we discuss corticosteroid insensitivity in asthma with an emphasis on mechanisms that contribute to persistent inflammation and diminished lung function in older individuals.
Clinical and Functional Characteristics of the E92K CFTR Gene Variant in the Russian and Turkish Population of People with Cystic Fibrosis
Authors: Kondratyeva Elena , Melyanovskaya Yuliya , Bulatenko Nataliya , Davydenko Ksenia , Filatova Alexandra , Efremova Anna , Skoblov Mikhail , Bukharova Tatiana , Sherman Viktoriya , Voronkova Anna , Zhekaite Elena , Krasovskiy Stanislav , Amelina Elena , Petrova Nika , Polyakov Alexander , Adyan Tagui , Starinova Marina , Krasnova Maria , Vasilyev Andrey , Makhnach Oleg , Zinchenko Rena , Kutsev Sergey , Gokdemir Yasemin , Karadag Bülent , Goldshtein Dmitry
Published: March 2023

The pathogenic variant E92K (c.274G > A) of the CFTR gene is rare in America and Europe, but it is common for people with cystic fibrosis from Russia and Turkey. We studied the effect of the E92K genetic variant on the CFTR function. The function of the CFTR channel was studied using the intestinal current measurements (ICM) method. The effects of CFTR modulators on the restoration of the CFTR function were studied in the model of intestinal organoids. To assess the effect of E92K on pre-mRNA splicing, the RT-PCR products obtained from patients’ intestinal organoid cultures were analyzed. Patients with the genetic variant E92K are characterized by an older age of diagnosis compared to homozygotes F508del and a high frequency of pancreatic sufficiency. The results of the sweat test and the ICM method showed partial preservation of the function of the CFTR channel. Functional analysis of CFTR gene expression revealed a weak effect of the E92K variant on mRNA-CFTR splicing. Lumacaftor (VX-809) has been shown to restore CFTR function in an intestinal organoid model, which allows us to consider the E92K variant as a promising target for therapy with CFTR correctors.
Dental Follicle Stem Cells Promote Periodontal Regeneration through Periostin-Mediated Macrophage Infiltration and Reprogramming in an Inflammatory Microenvironment
Authors: Wei Xiuqun , Guo Shujuan , Liu Qian , Liu Li , Huo Fangjun , Wu Yafei , Tian Weidong
Published: March 2023

Dental follicle stem cells (DFSCs) have been verified to promote periodontal regeneration in an inflammatory microenvironment. When coping with inflammatory stimulation, DFSCs highly express periostin, a bioactive molecule closely related to periodontal homeostasis. It is worth exploring whether and how periostin plays a role in the promotion of periodontal regeneration by DFSCs. By tracking the fate of DFSCs, it was found that DFSCs significantly contributed to periodontal regeneration in rat periodontal defects while they had a low survival rate. They highly expressed periostin and improved the immune microenvironment in the defect area, especially via the recruitment and reprogramming of macrophages. Silencing periostin attenuated the effects of DFSCs in promoting periodontal regeneration and regulating macrophages. Recombinant human periostin (rhPeriostin) could not only directly promote macrophage reprogramming through the integrin αM/phosphorylated extracellular signal-regulated kinase (p-Erk)/Erk signaling pathway, but it also exhibited the potential to promote periodontal regeneration in rats when loaded in a collagen matrix. These results indicated that periostin is actively involved in the process by which DFSCs promote periodontal regeneration through the regulation of macrophages and is a promising molecular agent to promote periodontal regeneration. This study provides new insight into the mechanism by which DFSCs promote periodontal regeneration and suggests a new approach for periodontal regeneration therapy.
3D Bioprinting for Next-Generation Personalized Medicine
Authors: Lam Ethan Hau Yin , Yu Fengqing , Zhu Sabrina , Wang Zongjie
Published: March 2023

In the past decade, immense progress has been made in advancing personalized medicine to effectively address patient-specific disease complexities in order to develop individualized treatment strategies. In particular, the emergence of 3D bioprinting for in vitro models of tissue and organ engineering presents novel opportunities to improve personalized medicine. However, the existing bioprinted constructs are not yet able to fulfill the ultimate goal: an anatomically realistic organ with mature biological functions. Current bioprinting approaches have technical challenges in terms of precise cell deposition, effective differentiation, proper vascularization, and innervation. This review introduces the principles and realizations of bioprinting with a strong focus on the predominant techniques, including extrusion printing and digital light processing (DLP). We further discussed the applications of bioprinted constructs, including the engraftment of stem cells as personalized implants for regenerative medicine and in vitro high-throughput drug development models for drug discovery. While no one-size-fits-all approach to bioprinting has emerged, the rapid progress and promising results of preliminary studies have demonstrated that bioprinting could serve as an empowering technology to resolve critical challenges in personalized medicine.
Determination of Selected Isoquinoline Alkaloids from Chelidonium majus, Mahonia aquifolium and Sanguinaria canadensis Extracts by Liquid Chromatography and Their In Vitro and In Vivo Cytotoxic Activity against Human Cancer Cells
Authors: Tuzimski Tomasz , Petruczynik Anna , Plech Tomasz , Kaproń Barbara , Makuch-Kocka Anna , Szultka-Młyńska Małgorzata , Misiurek Justyna , Buszewski Bogusław , Waksmundzka-Hajnos Monika
Published: March 2023

The search for new substances with cytotoxic activity against various cancer cells, especially cells that are very resistant to currently used chemotherapeutic agents, such as melanoma cells, is a very important scientific aspect. We investigated the cytotoxic effect of Chelidonium majus, Mahonia aquifolium and Sanguinaria canadensis extracts obtained from different parts of these plants collected at various vegetation stages on FaDu, SCC-25, MCF-7, and MDA-MB-231 cancer cells. Almost all the tested extracts showed higher cytotoxicity against these cancer cells than the anticancer drug etoposide. The highest cytotoxicity against the FaDu, SCC-25, MCF-7 and MDA-MB-231 cancer cell lines was obtained for the Sanguinaria candensis extract collected before flowering. The cytotoxicity of extracts obtained from different parts of Chelidonium majus collected at various vegetation stages was also evaluated on melanoma cells (A375, G361 and SK-MEL-3). The highest cytotoxic activity against melanoma A375 cells was observed for the Chelidonium majus root extract, with an IC50 of 12.65 μg/mL. The same extract was the most cytotoxic against SK-MEL-3 cells (IC50 = 1.93 μg/mL), while the highest cytotoxic activity against G361 cells was observed after exposure to the extract obtained from the herb of the plant. The cytotoxic activity of Chelidonium majus extracts against melanoma cells was compared with the cytotoxicity of the following anticancer drugs: etoposide, cisplatin and hydroxyurea. In most cases, the IC50 values obtained for the anticancer drugs were higher than those obtained for the Chelidonium majus extracts. The most cytotoxic extract obtained from the root of Chelidonium majus was selected for in vivo cytotoxic activity investigations using a Danio rerio larvae xenograft model. The model was applied for the first time in the in vivo investigations of the extract’s anticancer potential. The application of Danio rerio larvae xenografts in cancer research is advantageous because of the transparency and ease of compound administration, the small size and the short duration and low cost of the experiments. The results obtained in the xenograft model confirmed the great effect of the investigated extract on the number of cancer cells in a living organism. Our investigations show that the investigated plant extracts exhibit very high cytotoxic activity and can be recommended for further experiments in order to additionally confirm their potential use in the treatment of various human cancers.
Role of Berberine Thermosensitive Hydrogel in Periodontitis via PI3K/AKT Pathway In Vitro
Authors: Wang Chang , Liu Chang , Liang Chen , Qu Xingyuan , Zou Xinying , Du Siyu , Zhang Qian , Wang Lei
Published: March 2023

Periodontitis is a long-term inflammatory illness and a leading contributor to tooth loss in humans. Due to the influence of the anatomic parameters of teeth, such as root bifurcation lesions and the depth of the periodontal pocket, basic periodontal treatment on its own often does not completely obliterate flora microorganisms. As a consequence, topical medication has become a significant supplement in the treatment of chronic periodontitis. Berberine (BBR) has various pharmacological effects, such as hypoglycemic, antitumor, antiarrhythmic, anti-inflammatory, etc. The target of our project is to develop a safe and non-toxic carrier that can effectively release berberine, which can significantly reduce periodontal tissue inflammation, and to investigate whether berberine thermosensitive hydrogel can exert anti-inflammatory and osteogenic effects by modulating phosphatifylinositol-3-kinase/Protein Kinase B (PI3K/AKT) signaling pathway. Consequently, firstly berberine temperature-sensitive hydrogel was prepared, and its characterizations showed that the mixed solution gelated within 3 min under 37 °C with a hole diameter of 10–130 µm, and the accumulation of berberine release amounted to 89.99% at 21 days. CCK-8 and live-dead cell staining results indicated that this hydrogel was not biotoxic, and it is also presumed that the optimum concentration of berberine is 5 µM, which was selected for subsequent experiments. Real-time polymerase chain reaction (qRT-PCR) and Western blotting (WB)results demonstrated that inflammatory factors, as well as protein levels, were significantly reduced in the berberine-loaded hydrogel group, and LY294002 (PI3K inhibitor) could enhance this effect (p < 0.05). In the berberine-loaded hydrogel group, osteogenesis-related factor levels and protein profiles were visibly increased, along with an increase in alkaline phosphatase expression, which was inhibited by LY294002 (p < 0.05). Therefore, berberine thermosensitive hydrogel may be an effective treatment for periodontitis, and it may exert anti-inflammatory and osteogenic effects through the PI3K/AKT signaling pathway.
PgMYB1 Positively Regulates Anthocyanin Accumulation by Activating PgGSTF6 in Pomegranate
Authors: Wang Zenghui , Yang Xuemei , Wang Chuanzeng , Feng Lijuan , Yin Yanlei , Li Jialin
Published: March 2023

The peel color of pomegranates is an important exterior quality that determines market value. Anthocyanins are biosynthesized in the cytosol and then transported to the vacuole for storage. However, the molecular mechanism that determines the color variation between red and white pomegranates remains unclear. In this study, we identified an R2R3-MYB protein (PgMYB1) that interacts with the PgGSTF6 promoter and regulates its transcriptional expression, thus promoting the accumulation of anthocyanins in pomegranate. The expression of PgMYB1 and PgGSTF6 was positively correlated with the anthocyanin content in red and white pomegranates. Further investigation showed that the knockdown of PgMYB1 in red pomegranate ‘Taishanhong’ (TSH), by the virus-induced gene-silencing system, inhibited anthocyanin accumulation. Together, our results indicate that PgMYB1 controls the transport of anthocyanin via PgGSTF6 and thus promotes anthocyanin accumulation in red pomegranates. Our results have a certain reference value for further clarifying the regulation of anthocyanin synthesis and transport in pomegranate fruits.
Investigation of the Entry Pathway and Molecular Nature of σ1 Receptor Ligands
Authors: Pascarella Gianmarco , Antonelli Lorenzo , Narzi Daniele , Battista Theo , Fiorillo Annarita , Colotti Gianni , Guidoni Leonardo , Morea Veronica , Ilari Andrea
Published: March 2023

The σ1 receptor (σ1-R) is an enigmatic endoplasmic reticulum resident transmembrane protein implicated in a variety of central nervous system disorders and whose agonists have neuroprotective activity. In spite of σ1-R’s physio-pathological and pharmacological importance, two of the most important features required to fully understand σ1-R function, namely the receptor endogenous ligand(s) and the molecular mechanism of ligand access to the binding site, have not yet been unequivocally determined. In this work, we performed molecular dynamics (MD) simulations to help clarify the potential route of access of ligand(s) to the σ1-R binding site, on which discordant results had been reported in the literature. Further, we combined computational and experimental procedures (i.e., virtual screening (VS), electron density map fitting and fluorescence titration experiments) to provide indications about the nature of σ1-R endogenous ligand(s). Our MD simulations on human σ1-R suggested that ligands access the binding site through a cavity that opens on the protein surface in contact with the membrane, in agreement with previous experimental studies on σ1-R from Xenopus laevis. Additionally, steroids were found to be among the preferred σ1-R ligands predicted by VS, and 16,17-didehydroprogesterone was shown by fluorescence titration to bind human σ1-R, with significantly higher affinity than the prototypic σ1-R ligand pridopidine in the same essay. These results support the hypothesis that steroids are among the most important physiological σ1-R ligands.
Lipoprotein (a), Inflammation, and Atherosclerosis
Authors: Di Fusco Stefania Angela , Maggioni Aldo Pietro , Scicchitano Pietro , Zuin Marco , D’Elia Emilia , Colivicchi Furio
Published: March 2023

Growing evidence has shown that high levels of lipoprotein (a) (Lp(a)) and chronic inflammation may be responsible for the residual risk of cardiovascular events in patients managed with an optimal evidence-based approach. Clinical studies have demonstrated a correlation between higher Lp(a) levels and several atherosclerotic diseases including ischemic heart disease, stroke, and degenerative calcific aortic stenosis. The threshold value of Lp(a) serum concentrations associated with a significantly increased cardiovascular risk is >125 nmol/L (50 mg/dL). Current available lipid-lowering drugs have modest-to-no impact on Lp(a) levels. Chronic inflammation is a further condition potentially implicated in residual cardiovascular risk. Consistent evidence has shown an increased risk of cardiovascular events in patients with high sensitivity C reactive protein (>2 mg/dL), an inflammation biomarker. A number of anti-inflammatory drugs have been investigated in patients with or at risk of cardiovascular disease. Of these, canakinumab and colchicine have been found to be associated with cardiovascular risk reduction. Ongoing research aimed at improving risk stratification on the basis of Lp(a) and vessel inflammation assessment may help refine patient management. Furthermore, the identification of these conditions as cardiovascular risk factors has led to increased investigation into diagnostic and therapeutic strategies targeting them in order to reduce atherosclerotic cardiovascular disease burden.
Outcomes in Patients with Pulmonary Arterial Hypertension Underwent Transcatheter Closure of an Atrial Septal Defect
Authors: Seol Jae-Hee , Jung Se-Yong , Lee Han-Byul , Kim Ah-Young , Kim Eun-Hwa , Min In-Kyung , Kim Nam-Kyun , Choi Jae-Young
Published: March 2023

Pulmonary arterial hypertension (PAH) related to an atrial septal defect (ASD) poses a challenge to transcatheter closure of an ASD (tcASD). We aimed to determine the predictors for remaining PAH (rPAH) post-tcASD. This retrospective study was conducted at a single tertiary university hospital. Adult patients with an ASD and PAH were divided into three groups according to pulmonary vascular resistance (PVR). Normalization of pulmonary atrial systolic pressure (PASP) was defined as an estimated right ventricular systolic pressure < 40 mmHg and was determined using transthoracic echocardiography. Among 119 patients, 80% showed PAH normalization post-tcASD. Normalization of PAH post-tcASD was observed in 100%, 56.2%, and 28.6% of patients in mild, moderate, and severe PVR groups, respectively. The patients’ New York Heart Association functional class improved. Multivariate logistic regression analysis showed that age and high PVR were significant risk factors for rPAH. A receiving operator curve analysis showed a PASP cutoff value > 67.5 mmHg to be predictive of rPAH post-tcASD, with an area under the curve value of 0.944 (sensitivity, 0.922; specificity 0.933). Most patients, including moderate-to-severe PAH patients, improved hemodynamically and clinically with tcASD. Since patients with severe PAH are at a risk of rPAH, tcASD should be performed by selecting the patient carefully based on pre-procedure medication, a vasoreactivity test, and a balloon occlusion test.
A Pilot Cross-Sectional Study on the Level of Depression and Physical Activity among Students in Poland and Portugal in the Second Year of the COVID-19 Pandemic
Authors: Zalewska Anna , Gałczyk Monika , Sobolewski Marek , Fernandes Hélder
Published: March 2023

Objectives: The aim of the study was to make a preliminary assessment of depression rates and levels of physical activity and the association between physical activity and depression among Polish and Portuguese students in the second year of the COVID-19 pandemic. Methods: A web-based online survey was conducted among students in Poland and Portugal (398 respondents—229 from Poland and 169 from Portugal) aged between 17 and 26 in October 2022. The level of depression was assessed by the Beck Depression Inventory (BDI) in Polish and in Portuguese. The level of physical activity was measured by the International Physical Activity Questionnaire (IPAQ) in Polish and in Portuguese. Results: Overall, 20–35% of respondents suffered from moderate depression, with a lower proportion among men. There were no differences in the percentage distribution of depression classification between Polish and Portuguese students. A decrease in the severity of depression was observed with increasing physical activity. In both countries, walking more often had the strongest effect on reducing depression scores. Conclusions: The continued and alarming prevalence of depressive disorders among university students in the year after the pandemic began had a significant impact on this population—further research on the impact of physical activity on mental health in different populations should be conducted to adjust the optimal level of physical activity for alleviating negative psychiatric symptoms.
Predictors for Unsuccessful Reductions in Hemodialysis Frequency during the Pandemic
Authors: Anumas Suthiya , Kunawathanakul Sithichai , Tantiyavarong Pichaya , Krisanapan Pajaree , Pattharanitima Pattharawin
Published: March 2023

Background and Objectives: Patients receiving in-center hemodialysis are at a high risk of coronavirus disease 2019 (COVID-19) infection. A reduction in hemodialysis frequency is one of the proposed measures for preventing COVID-19 infection. However, the predictors for determining an unsuccessful reduction in hemodialysis frequency are still lacking. Materials and Methods: This retrospective observational study enrolled patients who were receiving long-term thrice-weekly hemodialysis at the Thammasat University Hospital in 2021 and who decreased their dialysis frequency to twice weekly during the COVID-19 outbreak. The outcomes were to determine the predictors and a prediction model of unsuccessful reduction in dialysis frequency at 4 weeks. Bootstrapping was performed for the purposes of internal validation. Results: Of the 161 patients, 83 patients achieved a dialysis frequency reduction. Further, 33% and 82% of the patients failed to reduce their dialysis frequency at 4 and 8 weeks, respectively. The predictors for unsuccessful reduction were diabetes, congestive heart failure (CHF), pre-dialysis overhydration, set dry weight (DW), DW from bioelectrical impedance analysis, and the mean pre- and post-dialysis body weight. The final model including these predictors demonstrated an AUROC of 0.763 (95% CI 0.654–0.866) for the prediction of an unsuccessful reduction. Conclusions: The prediction score involving diabetes, CHF, pre-dialysis overhydration, DW difference, and net ultrafiltration demonstrated a good performance in predicting an unsuccessful reduction in hemodialysis frequency at 4 weeks.
A 0.6 V Bulk-Driven Class-AB Two-Stage OTA with Non-Tailed Differential Pair
Authors: Ballo Andrea , Grasso Alfio Dario , Pennisi Salvatore
Published: March 2023

This work presents a two-stage operational transconductance amplifier suitable for sub-1 V operation. This characteristic is achieved thanks to the adoption of a bulk-driven non-tailed differential pair. Local positive feedback is exploited to boost the equivalent transconductance of the first stage and the quasi-floating gate approach enables the class AB operation of the second stage. Implemented in a standard 180 nm CMOS technology and supplied at 0.6 V, the amplifier exhibits a 350 kHz gain bandwidth product and a phase margin of 69° while driving a 150 pF load. Compared to other solutions in the literature, the proposed one exhibits a considerable performance improvement, especially for large signal operation.
An Optimized Quantification Method for Marine Radioactivity Measurements: Application in the Southern Caspian Sea Using the KATERINA Underwater γ-Spectrometer
Authors: Tsabaris Christos , Androulakaki Effrossyni G. , Alexakis Stylianos
Published: March 2023

The underwater gamma-ray spectrometer KATERINA was calibrated in a special tank and then demonstrated in the Southern Caspian Sea to determine the radioactivity levels in seawater as well as in the sediment. The simulated marine efficiency of the in situ detection system was validated analyzing the high energetic (1764 keV of 214Bi and 2614 keV of 208Tl) gamma-ray peaks using their known activity concentration as determined from the low energy emissions. The analysis of the in situ gamma-ray spectra in a short acquisition time period provided quantitative data for 226Ra and 232Th progenies as well as for 40K. A satisfactory analysis was performed for quantifying the observed energy peaks in the seawater and sediment measurements acquired at the south coast of the Caspian Sea. The spectrum in the water tank was also analyzed using full spectrum analysis methods, and the reproduced spectrum was in a very good agreement with the experimental spectrum giving as an output the activity concentrations of the observed radionuclides.
Sedimentary Facies, Architectural Elements, and Depositional Environments of the Maastrichtian Pab Formation in the Rakhi Gorge, Eastern Sulaiman Ranges, Pakistan
Authors: Mehmood Mubashir , Naseem Abbas Ali , Saleem Maryam , Rehman Junaid ur , Kontakiotis George , Janjuhah Hammad Tariq , Khan Emad Ullah , Antonarakou Assimina , Khan Ihtisham , Rehman Anees ur , Siyar Syed Mamoon
Published: March 2023

An integrated study of sediments was conducted to examine the facies architecture and depositional environment of the Cretaceous Pab Formation, Rakhi Gorge, and Suleiman Ranges, Pakistan. This research focused on analyzing architectural elements and facies, which are not commonly studied in sedimentary basins in Pakistan. To identify lithofacies, outcrop analysis and section measurement were performed. The identified lithofacies were then categorized based on their depositional characteristics and facies associations, with a total of nine types identified within a stratigraphic thickness of approximately 480 m. These facies were mainly indicative of high-energy environments, although the specifics varied by location. Sedimentary structures such as planar and trough crossbedding, lamination, nodularity, load-casts, and fossil traces were found within these facies, indicating high-energy environments with a few exceptions in calm environments. The identified facies were grouped into seven architectural elements according to their depositional environments: delta-dominated elements, including laminated shale sheet elements (LS), fine sandstone elements (SF), planar cross-bedded sandstone elements (SCp), trace sandstone elements (ST), and paleosol elements (Pa); and river-dominated elements, including trough cross-bedded sandstone elements (SCt), channel deposit elements (CH), and paleosol elements (Pa). These architectural elements, along with their vertical and lateral relationships, indicate a transitional fluvio-deltaic environment within the Pab Formation. In conclusion, by interpreting facies and architectural elements, it is possible to gain a better understanding of the depositional history of the formation and the distribution of reservoir units.
The Effect of the Fillets on Submarine Wake Field and Propeller Unsteady Bearing Force
Authors: Li Xiang , Cai Wenyu , Ren Nianxin , Sun Shuai
Published: March 2023

The uniformity of the wake on the submarine propeller disk is the key factor affecting the vibration and noise performance of the propeller. To study the influence of the fillets on the uniformity of submarine wake field and propeller unsteady bearing force, the submarine resistance calculation and self-propulsion calculation were carried out using the Detached-Eddy Simulation (DES) method. The characteristics of the wake field and hydrodynamic performance of the original and improved plan were compared and analyzed in detail. The fillets improve the wake distribution at the propeller disk by reducing the horseshoe vortex, and the fillets mainly affect the flow field characteristics of the inner radius area. The fillets can effectively reduce the axial velocity pulsation and improve the quality of the wake field. The frequency–domain curve shows that the bearing force and torque pulsation value of the improved plan are smaller than that in the original one, indicating that the fillets are conducive to controlling the vibration and noise performance of the submarine propeller. The results show that the fillets can effectively improve the flow field quality in the wake fields and reduce the propeller unsteady bearing force.
Design, Analysis and Simulation of Microstrip Antenna Arrays with Flexible Substrate in Different Frequency, for Use in UAV-Assisted Marine Communications
Authors: Gómez Leopoldo , Ibrahim Ahmed S.
Published: March 2023

The maritime land–sea communication channel experiences multipath shadowing and fading due to ships, onshore and offshore structures, and reflections from the sea surface. When using low altitude antennas, the sea surface itself can block the propagation of radio waves when the first Fresnel zone is obstructed. The latter can occur within a few kilometres of the transmitter at microwave frequencies. Sea reflections are stronger than ground reflections due to the higher conductivity of the sea, leading to more interference problems. In this paper, a microwave frequency patch antenna array is analysed, designed, and simulated for a novel system to improve marine communications to be applied by unmanned aerial vehicles (UAVs). The patch antenna array with flexible substrate will be studied with different frequencies. In this way, the test will check and obtain the best characteristics for an antenna that is built into the UAV with CTS studio.
Robust Lane Detection Algorithm for Autonomous Trucks in Container Terminals
Authors: Vinh Ngo Quang , Kim Hwan-Seong , Long Le Ngoc Bao , You Sam-Sang
Published: March 2023

Container terminal automation offers many potential benefits, such as increased productivity, reduced cost, and improved safety. Autonomous trucks can lead to more efficient container transport. A novel lane detection method is proposed using score-based generative modeling through stochastic differential equations for image-to-image translation. Image processing techniques are combined with Density-Based Spatial Clustering of Applications with Noise (DBSCAN) and Genetic Algorithm (GA) to ensure fast and accurate lane positioning. A robust lane detection method can deal with complicated detection problems in realistic road scenarios. The proposed method is validated by a dataset collected from the port terminals under different environmental conditions; in addition, the robustness of the lane detection method with stochastic noise is tested.
Resilience Regulation Strategy for Container Port Supply Chain under Disruptive Events
Authors: Xu Bowei , Liu Weiting , Li Junjun
Published: March 2023

There are many inevitable disruptive events, such as the COVID-19 pandemic, natural disasters and geopolitical conflicts, during the operation of the container port supply chain (CPSC). These events bring ship delays, port congestion and turnover inefficiency. In order to enhance the resilience of the CPSC, a modified two-stage CPSC system containing a container pretreatment system (CPS) and a container handling system (CHS) is built. A two-dimensional resilience index is designed to measure its affordability and recovery. An adaptive fuzzy double-feedback adjustment (AFDA) strategy is proposed to mitigate the disruptive effects and regulate its dynamicity. The AFDA strategy consists of the first-level fuzzy logic control system and the second-level adaptive fuzzy adjustment system. Simulations show the AFDA strategy outperforms the original system, PID, and two pipelines for improved dynamic response and augmented resilience. This study effectively supports the operations manager in determining the proper control policies and resilience management with respect to indeterminate container waiting delay and allocation delay due to disruptive effects.
Pneumocystis jirovecii Pneumonia Diagnostic Approach: Real-Life Experience in a Tertiary Centre
Authors: Veintimilla Cristina , Álvarez-Uría Ana , Martín-Rabadán Pablo , Valerio Maricela , Machado Marina , Padilla Belén , Alonso Roberto , Diez Cristina , Muñoz Patricia , Marín Mercedes
Published: March 2023

Pneumocystis jirovecii pneumonia (PJP) in immunocompromised patients entails high mortality and requires adequate laboratory diagnosis. We compared the performance of a real time-PCR assay against the immunofluorescence assay (IFA) in the routine of a large microbiology laboratory. Different respiratory samples from HIV and non-HIV-infected patients were included. The retrospective analysis used data from September 2015 to April 2018, which included all samples for which a P. jirovecii test was requested. A total of 299 respiratory samples were tested (bronchoalveolar lavage fluid (n = 181), tracheal aspirate (n = 53) and sputum (n = 65)). Forty-eight (16.1%) patients fulfilled the criteria for PJP. Five positive samples (10%) had only colonization. The PCR test was found to have a sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of 96%, 98%, 90% and 99%, compared to 27%, 100%, 100% and 87%, for the IFA, respectively. PJ-PCR sensitivity and specificity were >80% and >90% for all tested respiratory samples. Median cycle threshold values in definite PJP cases were 30 versus 37 in colonized cases (p < 0.05). Thus, the PCR assay is a robust and reliable test for the diagnosis PJP in all respiratory sample types. Ct values of ≥36 could help to exclude PJP diagnosis.
There He Goes: The Influencer–Sports Journalism of Fabrizio Romano on Twitter and Its Implications for Professionalism
Authors: McEnnis Simon
Published: March 2023

Fabrizio Romano’s sizeable social media followings and role as influencer–sports journalist warrant attention. Romano, known for his catchphrase ‘Here We Go’, specialises in football transfers and produces multi-platform content. This study investigates how Romano’s Twitter practice informs professional understandings of sports journalism. A content and textual analysis (n = 494) was conducted of one month of Romano’s tweets and replies. Tweets were categorised according to markers of sports journalism practice. Results show Romano is professionalising sports journalism on social media and subverting understandings of personal branding through favouring objective news over subjective opinion and focusing on the professional rather than the personal. Romano’s transfer news prioritises major European football clubs, which is consistent with the trajectory of sports journalism on digital platforms. Play-by-play commentary is a minor aspect of Romano’s Twitter output, which contributes to the debate on the significance of game coverage to contemporary sports journalism. Tweets did not mention the human rights issues surrounding the World Cup starting in Qatar, even though this was a key talking point. This finding suggests that the normative assumption that sports journalists should scrutinise power and/or highlight social injustice does not always apply to all practitioners in all contexts, particularly where ultra-specialist settings are concerned.
Are Drought and Wind Force Driving Factors of Wind Erosion Climatic Erosivity in a Changing Climate? A Case Study in a Landlocked Country in Central Europe
Authors: Lackóová Lenka , Kaletová Tatiana , Halászová Klaudia
Published: March 2023

The intensity and frequency of occurrence of wind erosion have had an increasing tendency in recent years, exacerbating environmental and agricultural problems around the world. The question of whether climate change will have an accelerating impact on wind erosion might be answered by analyzing three driving parameters: wind erosion climatic erosivity (CE), standard precipitation index (SPI), and wind factor (Wf). A time series analysis of historical climatic data over a period of 58 years was performed using ArcGIS software and descriptive statistics, to detect spatiotemporal variations regarding climate change. The results of the analysis indicate that the number and intensity of drought periods are already increasing in Central Europe. Through the CE equation using the key indicators wind speed (U), temperature (T), humidity (r), and precipitation (P), we calculated decadal spatiotemporal variation and potential scenarios of climate change in terms of wind erosion intensity. The results of the study show that there has been a 1.75 °C increase in temperature since 1961 and fluctuating wind erosion intensity in recent decades. The frequency of drought periods has increased only slightly, but there has been an increase in the amount of precipitation in the last two decades of the study period, up to +6.63 and +6.53%. The wind analysis showed that mean maximum wind speed (Umaxmean) had a decreasing trend (R2 = 0.32), and the occurrence of erosive wind (Uer) (>5 m/s) exhibited seasonal changes toward spring. Wf exhibited a rise of 11.86 and 3.66% in the first two decades of the study period, followed by a decline of 8.49% in the last decade. CE analysis indicated oscillation in both directions, with decadal changes ranging between −16.95 and +15.21%. Wind erosion is becoming a more significant issue in Central Europe because of climate change, and the situation could worsen in the future. This study provides valuable insights into the impact of climate change on wind erosion in Europe and highlights the need for effective measures to mitigate its effects.
Effects of Urban Form on Ambient Air Filter Noise Exposure in Open Areas
Authors: Lou Huading , Zhao Yuchen , Rong Ningning , Min Hequn
Published: March 2023

Although ambient air filters are commonly used to improve air quality in urban areas, their operation often produces significant noise levels. In this study, we investigated and addressed the issue of ambient air filter noise exposure in open areas of Chinese cities, with a focus on various typical urban forms. Firstly, fifteen common urban blocks with different forms and of 250 × 250 m each were chosen as sample sites, for which urban form indices and noise distribution indices were defined. Then noise mapping was conducted to investigate air filter noise exposure in open areas and the effects of urban form indices on noise distribution indices. Results show that urban form has considerable effects on filter noise exposure in open areas. Among the six urban form indices examined, the distance between the first-row building and the air filter was found to be the most critical factor affecting noise levels with the highest correlation coefficient (R = 0.754). The orientation of the first-row building shows the significant resisting effect on both average and background noise levels. Furthermore, the resisting effect of first-row buildings can create a maximum reduction of 12.0 dB (A) for peak noise. These indices could be profiled and used as an “a priori” tool for urban sound environment planning.