bluuebunny/crossref_metadata_2025 huggingface dataset:
Glial Chloride Homeostasis Under Transient Ischemic Stress
Authors: Engels Miriam , Kalia Manu , Rahmati Sarah , Petersilie Laura , Kovermann Peter , van Putten Michel J. A. M. , Rose Christine R. , Meijer Hil G. E. , Gensch Thomas , Fahlke Christoph
Published: September 2021

High water permeabilities permit rapid adjustments of glial volume upon changes in external and internal osmolarity, and pathologically altered intracellular chloride concentrations ([Cl–]int) and glial cell swelling are often assumed to represent early events in ischemia, infections, or traumatic brain injury. Experimental data for glial [Cl–]int are lacking for most brain regions, under normal as well as under pathological conditions. We measured [Cl–]int in hippocampal and neocortical astrocytes and in hippocampal radial glia-like (RGL) cells in acute murine brain slices using fluorescence lifetime imaging microscopy with the chloride-sensitive dye MQAE at room temperature. We observed substantial heterogeneity in baseline [Cl–]int, ranging from 14.0 ± 2.0 mM in neocortical astrocytes to 28.4 ± 3.0 mM in dentate gyrus astrocytes. Chloride accumulation by the Na+-K+-2Cl– cotransporter (NKCC1) and chloride outward transport (efflux) through K+-Cl– cotransporters (KCC1 and KCC3) or excitatory amino acid transporter (EAAT) anion channels control [Cl–]int to variable extent in distinct brain regions. In hippocampal astrocytes, blocking NKCC1 decreased [Cl–]int, whereas KCC or EAAT anion channel inhibition had little effect. In contrast, neocortical astrocytic or RGL [Cl–]int was very sensitive to block of chloride outward transport, but not to NKCC1 inhibition. Mathematical modeling demonstrated that higher numbers of NKCC1 and KCC transporters can account for lower [Cl–]int in neocortical than in hippocampal astrocytes. Energy depletion mimicking ischemia for up to 10 min did not result in pronounced changes in [Cl–]int in any of the tested glial cell types. However, [Cl–]int changes occurred under ischemic conditions after blocking selected anion transporters. We conclude that stimulated chloride accumulation and chloride efflux compensate for each other and prevent glial swelling under transient energy deprivation.
Circuits and Synapses: Hypothesis, Observation, Controversy and Serendipity – An Opinion Piece
Authors: Thomson Alex M.
Published: September 2021

More than a century of dedicated research has resulted in what we now know, and what we think we know, about synapses and neural circuits. This piece asks to what extent some of the major advances – both theoretical and practical – have resulted from carefully considered theory, or experimental design: endeavors that aim to address a question, or to refute an existing hypothesis. It also, however, addresses the important part that serendipity and chance have played. There are cases where hypothesis driven research has resulted in important progress. There are also examples where a hypothesis, a model, or even an experimental approach – particularly one that seems to provide welcome simplification – has become so popular that it becomes dogma and stifles advance in other directions. The nervous system rejoices in complexity, which should neither be ignored, nor run from. The emergence of testable “rules” that can simplify our understanding of neuronal circuits has required the collection of large amounts of data that were difficult to obtain. And although those collecting these data have been criticized for not advancing hypotheses while they were “collecting butterflies,” the beauty of the butterflies always enticed us toward further exploration.
Oromandibular Dystonia: A Clinical Examination of 2,020 Cases
Authors: Scorr Laura M. , Factor Stewart A. , Parra Sahyli Perez , Kaye Rachel , Paniello Randal C. , Norris Scott A. , Perlmutter Joel S. , Bäumer Tobias , Usnich Tatiana , Berman Brian D. , Mailly Marie , Roze Emmanuel , Vidailhet Marie , Jankovic Joseph , LeDoux Mark S. , Barbano Richard , Chang Florence C. F. , Fung Victor S. C. , Pirio Richardson Sarah , Blitzer Andrew , Jinnah H. A.
Published: September 2021

Objective: The goal of this study is to better characterize the phenotypic heterogeneity of oromandibular dystonia (OMD) for the purpose of facilitating early diagnosis.

Methods: First, we provide a comprehensive summary of the literature encompassing 1,121 cases. Next, we describe the clinical features of 727 OMD subjects enrolled by the Dystonia Coalition (DC), an international multicenter cohort. Finally, we summarize clinical features and treatment outcomes from cross-sectional analysis of 172 OMD subjects from two expert centers.

Results: In all cohorts, typical age at onset was in the 50s and 70% of cases were female. The Dystonia Coalition cohort revealed perioral musculature was involved most commonly (85%), followed by jaw (61%) and tongue (17%). OMD more commonly appeared as part of a segmental dystonia (43%), and less commonly focal (39%) or generalized (10%). OMD was found to be associated with impaired quality of life, independent of disease severity. On average, social anxiety (LSA score: 33 ± 28) was more common than depression (BDI II score: 9.7 ± 7.8). In the expert center cohorts, botulinum toxin injections improved symptom severity by more than 50% in ~80% of subjects, regardless of etiology.

Conclusions: This comprehensive description of OMD cases has revealed novel insights into the most common OMD phenotypes, pattern of dystonia distribution, associated psychiatric disturbances, and effect on QoL. We hope these findings will improve clinical recognition to aid in timely diagnosis and inform treatment strategies.
Diagnostic Evaluation of Visual Snow
Authors: Vaphiades Michael S. , Grondines Brendan , Cooper Kasey , Gratton Sean , Doyle Jennifer
Published: September 2021

Introduction: To determine which patients with visual snow (VS) and VS syndrome (VSS) require standard ophthalmologic testing including automated visual field and which patients require further testing such as macular spectral domain optical coherence tomography (SD-OCT), electrophysiology, and neuroimaging.

Materials and Methods: We retrospectively reviewed 52 consecutive patients at three institutions with VS and VSS including the University of Alabama, Callahan Eye Hospital, the University of Missouri-Kansas City School of Medicine, and the Little Rock Eye Clinic from the years 2015 to 2021. We collected historical information, examination findings, ophthalmic testing, electrophysiology, and neuroimaging.

Results: Of the 52 patients with VS and VSS, eight of the 52 cases met the clinical criteria for VSS. The ages ranged from 7 to 79 years, with a mean age of 25 years (SD = 14.0). There were 22 males and 30 females. Color vision was tested in 51 cases and was normal in 47 cases (92%). A funduscopic exam was performed in all 52 cases and was normal in 46 cases (88%). The macular SD-OCT was normal in all of the 19 cases that it was performed (100%). A Humphrey visual field was performed in 50 cases and was normal in 43 (86%). A visually evoked potential (VEP) was normal in 18 of the 19 cases where it was obtained (95%). The full-field electroretinography (ffERG) was obtained in 28 cases and was normal in 25 (89%). The multifocal electroretinography (mfERG) was normal in 11 of 12 cases (92%). Only four patients accounted for all of the abnormal electrophysiological tests. In the 37 cases that had an MRI, 29 were normal (78%). Only one patient revealed a lesion in the visual pathway (right optic nerve enhancement in an optic neuritis patient).

Conclusions: Patients with VS and VSS, if typical in presentation and with normal testing, do not require a workup beyond a thorough history, neuro-ophthalmologic examination, and automated perimetry. If this testing is abnormal, then ancillary testing is required.
Lactic Dehydrogenase to Albumin Ratio Is Associated With the Risk of Stroke-Associated Pneumonia in Patients With Acute Ischemic Stroke
Authors: Yan Dan , Huang Qiqi , Dai Caijun , Ren Wenwei , Chen Siyan
Published: September 2021

Background:Stroke-associated pneumonia (SAP) is one of the common complications of stroke patients. Higher lactic dehydrogenase (LDH) and lower albumin levels were associated with SAP, but the contribution of the LDH to albumin ratio (LAR) to the risk of SAP in acute ischemic stroke (AIS) patients remained unclear.

Methods:A total of 3173 AIS patients were included in this study, divided into SAP (n= 417) and non-SAP groups (n= 2756). Characteristics were compared between these two groups. The receiver operating characteristic curves (ROC) were used to evaluate the discrimination ability of the LAR, LDH, and albumin levels in predicting SAP. Logistic regression analysis was furtherly adopted to estimate the association between LAR and SAP. We also used the restricted cubic spline (RCS) to clarify the relationship between LAR and the risk of SAP.

Results:LAR in the SAP group was significantly higher than that of the non-SAP group (8.75 ± 4.58 vs. 6.10 ± 2.55,P< 0.001). According to the results of ROC, LAR had the highest prognostic accuracy compared to LDH and albumin (P< 0.05). Besides, the logistic regression model showed that higher LAR (LAR > 6.75) were more vulnerable to SAP (OR, 2.80; 95% CI, 2.18–3.59,P< 0.001), controlling the confounders. The RCS model showed that there was a non-linear relationship between LAR and the risk of SAP.

Conclusion:High LAR was associated with an increased risk of SAP in patients with AIS. LAR may be a potential predictor for the incidence of SAP. Appropriate prevention measures were needed in patients with high LAR (LAR > 6.75).
Accurately Locating Metastatic Foci in Lymph Nodes With Lugol’s Iodine-Enhanced Micro-CT Imaging
Authors: Xia Cheng-Wan , Hu Shi-Qi , Zhou Qun-Zhi , Gan Rong-Lin , Pan Jiong-Ru , Zhang Qian , Pu Yu-Mei , Chen Shen , Hu Qin-Gang , Wang Yu-Xin
Published: September 2021

Background

Accurate evaluation of lymph node (LN) status is the key factor to determine the treatment and evaluate prognosis for patients with cancer. However, traditional pathological examination resulted in a 30% false-negative rate of detection of metastases in LNs. This study aimed to utilize Lugol’s iodine (I2-IK)-enhanced micro-CT imaging to reveal the 3-dimensional structure of regional LNs and decrease the false-negative rate in pathological examination.

Methods

To explore the feasibility of I2-IK-enhanced micro-CT imaging in locating metastatic lesion in LNs, nonmetastatic and metastatic LNs from mice were used to mimic the imaging process. Then, the LNs from oral squamous cell carcinoma (OSCC) patients were applied to verify the value of I2-IK-enhanced micro-CT imaging in revealing LN structure and locating metastatic lesions in LNs. The glycogen content in nonmetastatic and metastatic LNs was further detected by the use of a glycogen assay kit and periodic acid–Schiff (PAS) staining to explain the imaging differences between them.

Results

In nude mice, 0.5% I2-IK staining for 4 h was the best parameter for normal LN. The metastatic foci in metastatic LNs were also clearly outlined in this condition. For nonmetastatic LNs from patients with OSCC, 1% I2-IK staining for 12 h was the best parameter. However, due to the increased volume of metastatic LNs, the image effect of 3% I2-IK staining for 12 h was superior to 1% I2-IK staining [tumor background ratio (TBR), 3% vs. 1%, 1.89 ± 0.10 vs. 1.27 ± 0.07, p < 0.001]. Compared with subsequent pathological sections, we found the CT intensity of metastatic foci in LNs and muscle tissues was significantly higher than in nonmetastatic regions. Meanwhile, the glycogen content of metastatic foci in LNs detected was also significantly higher than in nonmetastatic region.

Conclusions

I2-IK-enhanced micro-CT imaging could identify the spatial location of metastatic foci in LNs. This will be an effective method to assist in decreasing the LN false-negative rate for cancer pathology.
Individualized Prediction of Survival Benefits of Pancreatectomy Plus Chemotherapy in Patients With Simultaneous Metastatic Pancreatic Cancer
Authors: Nie Duorui , Lai Guihua , An Guilin , Wu Zhuojun , Lei Shujun , Li Jing , Cao Jianxiong
Published: September 2021

Background

Metastatic pancreatic cancer (mPC) is a highly lethal malignancy with poorer survival. However, chemotherapy alone was unable to maintain long‐term survival. This study aimed to evaluate the individualized survival benefits of pancreatectomy plus chemotherapy (PCT) for mPC.

Methods

A total of 4546 patients with mPC from 2004 to 2015 were retrieved from the Surveillance, Epidemiology, and End Results database. The survival curve was calculated using the Kaplan-Meier method and differences in survival curves were tested using log-rank tests. Cox proportional hazards regression analyses were performed to evaluate the prognostic value of involved variables. A new nomogram was constructed to predict overall survival based on independent prognosis factors. The performance of the nomogram was measured by concordance index, calibration plot, and area under the receiver operating characteristic curve.

Results

Compared to pancreatectomy or chemotherapy alone, PCT can significantly improve the prognosis of patients with mPC. In addition, patients with well/moderately differentiated tumors, age ≤66 years, tumor size ≤42 mm, or female patients were more likely to benefit from PCT. Multivariate analysis showed that age at diagnosis, sex, marital status, grade, tumor size, and treatment were independent prognostic factors. The established nomogram has a good ability to distinguish and calibrating.

Conclusion

PCT can prolong survival in some patients with mPC. Our nomogram can individualize predict OS of pancreatectomy combined with chemotherapy in patients with concurrent mPC.
Axitinib and HDAC Inhibitors Interact to Kill Sarcoma Cells
Authors: Roberts Jane L. , Booth Laurence , Poklepovic Andrew , Dent Paul
Published: September 2021

We have extended our analyses of HDAC inhibitor biology in sarcoma. The multi-kinase inhibitor axitinib interacted with multiple HDAC inhibitors to kill sarcoma cells. Axitinib and HDAC inhibitors interacted in a greater than additive fashion to inactivate AKT, mTORC1 and mTORC2, and to increase Raptor S722/S792 phosphorylation. Individually, all drugs increased phosphorylation of ATM S1981, AMPKα T172, ULK1 S317 and ATG13 S318 and reduced ULK1 S757 phosphorylation; this correlated with enhanced autophagic flux. Increased phosphorylation of ULK1 S317 and of Raptor S722/S792 required ATM-AMPK signaling. ULK1 S757 is a recognized site for mTORC1 and knock down of either ATM or AMPKα reduced the drug-induced dephosphorylation of this site. Combined exposure of cells to axitinib and an HDAC inhibitor significantly reduced the expression of HDAC1, HDAC2, HDAC3, HDAC4, HDAC6 and HDAC7. No response was observed for HDACs 10 and 11. Knock down of ULK1, Beclin1 or ATG5 prevented the decline in HDAC expression, as did expression of a constitutively active mTOR protein. Axitinib combined with HDAC inhibitors enhanced expression of Class I MHCA and reduced expression of PD-L1 which was recapitulated via knock down studies, particularly of HDACs 1 and 3. In vivo, axitinib and the HDAC inhibitor entinostat interacted to significantly reduce tumor growth. Collectively our findings support the exploration of axitinib and HDAC inhibitors being developed as a novel sarcoma therapy.
Birt-Hogg-Dubé Syndrome and Hereditary Leiomyomatosis and Renal Cell Carcinoma Syndrome: An Effective Multidisciplinary Approach to Hereditary Renal Cancer Predisposing Syndromes
Authors: Al-Shinnag Mohammad , Marfan Helen , Susman Rachel , Wakeling Jan , Gustafson Sonja , Wood Simon , Mallett Andrew John
Published: September 2021

Aim

We aimed to describe and analyse clinical features, characteristics, and adherence to surveillance guidelines in an Australian Birt-Hogg-Dubé syndrome (BHD) and hereditary leiomyomatosis and renal cell cancer (HLRCC) cohort.

Methods

All identified patients with a diagnosis of BHD or HLRCC at RBWH 01/01/2014-01/09/2019 were included (HREC/17/QRBW/276). All patients were initially assessed and counselled by a clinical geneticist and then referred to an adult nephrologist. Baseline and incidental clinical variables were extracted and analysed.

Results

Fifty-seven patients were identified (28 BHD, 29 HLRCC) with a median age of 47 years. The median and cumulative follow-up were 1 and 99 years, respectively. Baseline renal MRI occurred in 40/57 patients, and 33/57 had regular MRI as per the national guidelines (eviQ). Of 18/57 without baseline imaging, nine were yet to have imaging, seven were lost follow-up, and two patients had logistic difficulties. RCC was diagnosed in 11/57 patients: two of 28 with BHD were diagnosed with RCC aged 73 and 77, both prior to commencement of surveillance. Nine of 29 patients with HLRCC were diagnosed with RCC (one of 29 during surveillance at 47 years of age) and eight of 29 prior to commencement of surveillance (11–55 years). Amongst BHD patients, cutaneous fibrofolliculomas were noted in 15 patients, lung cysts were detected in seven patients, spontaneous pneumothoraces in five patients, and parotid oncocytoma in two of 28. Amongst those with HLRCC, cutaneous leiomyomas were noted in 19/29, cutaneous leiomyosarcoma diagnosed in one of 29, and uterine fibroids in 13 female patients.

Conclusion

Evidence-based RCC screening in BHD and HLRCC cohort is feasible and able to identify incidental renal lesions. Multidisciplinary patient management enables expedited genetic counselling, diagnosis, longitudinal screening, and RCC management. The success of this clinical model warrants consideration of undertaking longitudinal screening of BHD and HLRCC patients by nephrologists.
The Efficacy and Safety of Infliximab in Refractory Noninfectious Uveitis: A Meta-Analysis of Observational Studies
Authors: Xiong Anji , Liu Deng , Chen Huini , Yang Guancui , Xiong Chen , Shuai Yu , He Linqian , Guo Zepeng , Zhang Liangwen , Yang Yuan , Cui Beibei , Shuai Shiquan
Published: September 2021

Background: Although infliximab has been recommended for the second-line treatment of seronegative spondyloarthropathy- or juvenile idiopathic arthritis-related uveitis, the issue of its systemic efficacy and safety in a broader diversity of refractory noninfectious uveitis is debatable. To assess the short-term and relatively long-term efficacy of infliximab in refractory noninfectious uveitis, we performed a systematic review and meta-analysis of observational studies.

Methods: PubMed, Cochrane Library, EMBASE, and Wanfang Med Online were systematically searched from January 2005 to March 2020. Two investigators independently assessed eligibility. Data were independently collected by two investigators. The pooled proportions were estimated with patients for intraocular inflammation control and improvement of visual acuity. Pooled proportions with 95% credible intervals were computed. Study homogeneity was investigated using I2 statistics to quantify the percentage of variation across studies. To pool the results, the Mantel–Haenszel fixed-effects or random-effects models were used.

Results: Of 2316 studies identified, 16 unique studies with 509 unique participants were included in the meta-analysis. The pooled proportions of intraocular inflammation control reached 92% (95% CI: 87%–98%; I2: 1%; p=0.42) and 95% (95% CI: 93%–97%; I2: 0%; p=0.91) in groups of ≤6- and ≥12-month follow-up durations. During the relatively long follow-up period, the pooled proportions of maintaining visual acuity stable or increasing at least one line reached 99% (95% CI: 96%–100%; I2: 0%; p=0.54) in the involved eyes. The corticosteroid-sparing effect of infliximab was also well demonstrated, with the proportion of corticosteroid-sparing success reaching 85.5% (112/131). Besides, about serious adverse events, 2.6% (13/500) of patients experienced hypersensitivity reactions, 2.4% (12/500) of patients experienced serious infections, 1.8% (9/500) of patients experienced autoimmune diseases, and 0.6% (3/500) of patients experienced neoplasia.

Conclusions: This meta-analysis provided evidence that infliximab might be a promising choice in controlling inflammatory activity, gaining visual acuity, and sparing corticosteroid use with relatively few side effects when applied in treating refractory noninfectious uveitis.

Systematic Review Registration: [website], identifier [registration number]
Molecular Mechanisms Underpinning the Circulation and Cellular Uptake of Mycobacterium ulcerans Toxin Mycolactone
Authors: Tello Rubio Bruno , Bugault Florence , Baudon Blandine , Raynal Bertrand , Brûlé Sébastien , Morel Jean-David , Saint-Auret Sarah , Blanchard Nicolas , Demangel Caroline , Guenin-Macé Laure
Published: September 2021

Mycolactone is a diffusible lipid toxin produced by Mycobacterium ulcerans, the causative agent of Buruli ulcer disease. Altough bacterially derived mycolactone has been shown to traffic from cutaneous foci of infection to the bloodstream, the mechanisms underpinning its access to systemic circulation and import by host cells remain largely unknown. Using biophysical and cell-based approaches, we demonstrate that mycolactone specific association to serum albumin and lipoproteins is necessary for its solubilization and is a major mechanism to regulate its bioavailability. We also demonstrate that Scavenger Receptor (SR)-B1 contributes to the cellular uptake of mycolactone. Overall, we suggest a new mechanism of transport and cell entry, challenging the dogma that the toxin enters host cells via passive diffusion.
Alcohol Abstinence Rescues Hepatic Steatosis and Liver Injury via Improving Metabolic Reprogramming in Chronic Alcohol-Fed Mice
Authors: Pi Aiwen , Jiang Kai , Ding Qinchao , Lai Shanglei , Yang Wenwen , Zhu Jinyan , Guo Rui , Fan Yibin , Chi Linfeng , Li Songtao
Published: September 2021

Background: Alcoholic liver disease (ALD) caused by chronic ethanol overconsumption is a common type of liver disease with a severe mortality burden throughout the world. The pathogenesis of ALD is complex, and no effective clinical treatment for the disease has advanced so far. Prolonged alcohol abstinence is the most effective therapy to attenuate the clinical course of ALD and even reverse liver damage. However, the molecular mechanisms involved in alcohol abstinence-improved recovery from alcoholic fatty liver remain unclear. This study aims to systematically evaluate the beneficial effect of alcohol abstinence on pathological changes in ALD.

Methods: Using the Lieber-DeCarli mouse model of ALD, we analysed whether 1-week alcohol withdrawal reversed alcohol-induced detrimental alterations, including oxidative stress, liver injury, lipids metabolism, and hepatic inflammation, by detecting biomarkers and potential targets.

Results: Alcohol withdrawal ameliorated alcohol-induced hepatic steatosis by improving liver lipid metabolism reprogramming via upregulating phosphorylated 5′-AMP -activated protein kinase (p-AMPK), peroxisome proliferator-activated receptor-α (PPAR-α), and carnitine palmitoyltransferase-1 (CPT-1), and downregulating fatty acid synthase (FAS) and diacylglycerol acyltransferase-2 (DGAT-2). The activities of antioxidant enzymes, including superoxide dismutase (SOD) and glutathione peroxidase (GSH-px), were significantly enhanced by alcohol withdrawal. Importantly, the abstinence recovered alcohol-fed induced liver injury, as evidenced by the improvements in haematoxylin and eosin (H&E) staining, plasma alanine aminotransferase (ALT) levels, and liver weight/body weight ratio. Alcohol-stimulated toll-like receptor 4/mitogen-activated protein kinases (TLR4/MAPKs) were significantly reversed by alcohol withdrawal, which might mechanistically contribute to the amelioration of liver injury. Accordingly, the hepatic inflammatory factor represented by tumour necrosis factor-alpha (TNF-α) was improved by alcohol abstinence.

Conclusion: In summary, we reported that alcohol withdrawal effectively restored hepatic lipid metabolism and reversed liver injury and inflammation by improving metabolism reprogramming. These findings enhanced our understanding of the biological mechanisms involved in the beneficial role of alcohol abstinence as an effective treatment for ALD.
A Systematic Narration of Some Key Concepts and Procedures in Plant Breeding
Authors: Yan Weikai
Published: September 2021

The goal of a plant breeding program is to develop new cultivars of a crop kind with improved yield and quality for a target region and end-use. Improved yield across locations and years means better adaptation to the climatic, soil, and management conditions in the target region. Improved or maintained quality renders and adds value to the improved yield. Both yield and quality must be considered simultaneously, which constitutes the greatest challenge to successful cultivar development. Cultivar development consists of two stages: the development of a promising breeding population and the selection of the best genotypes out of it. A complete breeder's equation was presented to cover both stages, which consists of three key parameters for a trait of interest: the population mean (μ), the population variability (σG), and the achieved heritability (h2 or H), under the multi-location, multi-year framework. Population development is to maximize μσG and progeny selection is to improve H. Approaches to improve H include identifying and utilizing repeatable genotype by environment interaction (GE) through mega-environment analysis, accommodating unrepeatable GE through adequate testing, and reducing experimental error via replication and spatial analysis. Related concepts and procedures were critically reviewed, including GGE (genotypic main effect plus genotype by environment interaction) biplot analysis, GGE + GGL (genotypic main effect plus genotype by location interaction) biplot analysis, LG (location-grouping) biplot analysis, stability analysis, spatial analysis, adequate testing, and optimum replication. Selection on multiple traits includes independent culling and index selection, for the latter GYT (genotype by yield*trait) biplot analysis was recommended. Genomic selection may provide an alternative and potentially more effective approach in all these aspects. Efforts were made to organize and comment on these concepts and procedures in a systematic manner.
Phosphoproteomic Analysis of Thermomorphogenic Responses in Arabidopsis
Authors: Shao Yu-Jian , Zhu Qiao-Yun , Yao Zi-Wei , Liu Jian-Xiang
Published: September 2021

Plants rapidly adapt to elevated ambient temperature by adjusting their growth and developmental programs. To date, a number of experiments have been carried out to understand how plants sense and respond to warm temperatures. However, how warm temperature signals are relayed from thermosensors to transcriptional regulators is largely unknown. To identify new early regulators of plant thermo-responsiveness, we performed phosphoproteomic analysis using TMT (Tandem Mass Tags) labeling and phosphopeptide enrichment with Arabidopsis etiolated seedlings treated with or without 3h of warm temperatures (29°C). In total, we identified 13,160 phosphopeptides in 5,125 proteins with 10,700 quantifiable phosphorylation sites. Among them, 200 sites (180 proteins) were upregulated, while 120 sites (87 proteins) were downregulated by elevated temperature. GO (Gene Ontology) analysis indicated that phosphorelay-related molecular function was enriched among the differentially phosphorylated proteins. We selected ATL6 (ARABIDOPSIS TOXICOS EN LEVADURA 6) from them and expressed its native and phosphorylation-site mutated (S343A S357A) forms in Arabidopsis and found that the mutated form of ATL6 was less stable than that of the native form both in vivo and in cell-free degradation assays. Taken together, our data revealed extensive protein phosphorylation during thermo-responsiveness, providing new candidate proteins/genes for studying plant thermomorphogenesis in the future.
Spatial Separation and Working Memory Capacity Affect Selective Visual Attention in the Periphery
Authors: Klatt Stefanie , Smeeton Nicholas J.
Published: September 2021

The current study aimed to examine the effects of spatial separation and working memory capacity on selective visual attention. We investigated differences in the ability to identify the two covertly attended stimuli that appeared either along one of the meridians (e.g., both along the horizontal) or along two of the meridians (e.g., one along the horizontal and one along the vertical) in the attention-window task. Two visual stimuli in the periphery could be perceived along wider extents of the attentional focus’ meridians (horizontal, vertical, and diagonal) when they were located along the same meridian (e.g., horizontal) compared to two different ones (e.g., horizontal and vertical). Subjects with high working memory capacity outperformed subjects with lower working memory capacity in both conditions and stimuli presented on two meridians were less accurately perceived. The findings support the proposal that individual differences in working memory capacity are important for selective spatial visual attention.
Piano Education of Children Using Musical Instrument Recognition and Deep Learning Technologies Under the Educational Psychology
Authors: Li Huizi
Published: September 2021

The objective of the study was to enhance quality education in the traditional pre-school piano education. Deep Learning (DL) technology is applied to piano education of children to improve their interest in learning music. Firstly, the problems of the traditional piano education of children were analyzed with the teaching patterns discussed under educational psychology, and a targeted music education plan was established. Secondly, musical instrument recognition technology was introduced, and the musical instrument recognition model was implemented based on DL. Thirdly, the proposed model was applied to the piano education of children to guide the music learning of students and improve their interest in piano learning. The feature recognition and acquisition of the proposed model were improved. Finally, the different teaching patterns were comparatively analyzed through the Questionnaire Survey (QS). The experimental results showed that the instrument recognition accuracy of Hybrid Neural Network (HNN) is 97.2%, and with the increase of iterations, the recognition error rate of the model decreases and stabilizes. Therefore, the proposed HNN based on DL for musical instrument recognition can accurately identify musical features. The QS results showed that the introduction of musical instrument recognition technology in the piano education of children can improve their interest in piano learning. Therefore, the establishment of the piano education patterns based on the piano education model can improve the effectiveness of teaching piano to students. This research provides a reference for the intelligentization of children's piano education.
Validation of the Chinese Version of the Procrastination at Work Scale
Authors: Wang Jiayan , Li Chaoping , Meng Xue , Liu Doudou
Published: September 2021

The purpose of this study was to validate the Chinese version of the Procrastination at Work Scale (PAWS), a recently developed scale aimed at assessing procrastination in the work context. We translated the PAWS into Chinese and conducted exploratory factor analysis on participants in sample A (N = 236), resulting in a two-factor solution consistent with the original PAWS. In sample B (N = 227), confirmatory factor analysis showed that a two-factor, bifactor model fit the data best. Configural, metric, and scalar invariance models were tested, which demonstrated that the Chinese version of the PAWS did not differ across groups by gender, age, education, or job position. Validity testing demonstrated that the scale relates to work engagement, counterproductive work behavior, task performance, workplace well-being, and organizational commitment. This study indicated that the Chinese version of the PAWS could be used in future research to measure procrastination at work in China.
Evaluation of User Satisfaction and Trust of Review Platforms: Analysis of the Impact of Privacy and E-WOM in the Case of TripAdvisor
Authors: Martínez-Navalón Juan-Gabriel , Gelashvili Vera , Gómez-Ortega Alba
Published: September 2021

Technological advances have had many advantages like an E-WOM (Electronic Word of Mouth) that has become a very important and powerful tool for users who wish to share their knowledge, experiences and emotions about a product or service. But the use of virtual platforms may affect the privacy of users data. This present study has a twofold objective: first check if the privacy of users when using TripAdvisor, the world’s largest travel platform, has an impact on their satisfaction and trust on the platform. Secondly, the relationship between E-WOM and the variables users trust and satisfaction when using TripAdvisor is examined. In order to achieve the objectives set out, the sample of 390 persons was analyzed. The PLS-SEM method has been used to process the data and test the hypotheses. The results of the analysis have shown that there is a positive and direct relationship between TripAdvisor users’ privacy and satisfaction. The direct and positive relationship between users’ E-WOM and their degree of satisfaction and trust toward the platform has also been confirmed. This study makes a significant contribution to the academic literature on the variables studied, as previous studies presented different results.
The Predictive Role of Chinese EFL Teachers’ Individual Self-Efficacy and Collective Efficacy in Their Work Engagement
Authors: Yang Jianbo
Published: September 2021

Both teacher individual self-efficacy (TSE) and collective efficacy (TCE) are indispensable since they impact the amount to which teachers are engaged in their work. Although several studies have been carried out considering the referred points, it seems to be a must to conduct such studies among Chinese teachers to measure the link between these three variables. In this study, the author has made endeavors to scrutinize the interplay among TSE, TCE, and work engagement (WE), and also the significant role of self-efficacy and collective efficacy in WE. Participants were 346 university professors and middle school teachers, from 25 provinces of China. Results substantiate that TSE and TCE predict teachers’ WE. In other words, the more efficacious a teacher is, the more he is engaged in his work. In the discussion part, the consistency between the current study and some other studies with the same topic is unpacked. Then, some limitations are discussed and further implications are suggested.
Shared Decision Making With Young People at Ultra High Risk of Psychotic Disorder
Authors: Simmons Magenta Bender , Brushe Mary , Elmes Aurora , Polari Andrea , Nelson Barnaby , Montague Alice
Published: September 2021

Introduction: While the majority of young people who meet the criteria for being considered at increased risk of psychosis do not go on to develop a psychotic disorder, young people are currently being identified and treated in early intervention services. Ethical concerns have been raised concerning the decision about whether or not to provide treatment, and if so, what type of treatment. This study sought to support young people themselves to make these decisions with support from their clinician through a shared decision-making approach, facilitated by an online decision aid.

Methods: This project used the International Patient Decision Aid Standards (IPDAS) to guide the development and piloting of an online decision aid across two phases: (1) qualitative, semi-structured focus groups with young people who were past clients and clinicians from an early psychosis service; and (2) pilot testing of the decision aid with clinicians and young people who were current clients to finalize the development.

Results: Issues discussed by clinicians in the focus group were grouped into three main areas: (1) engagement phase; (2) assessment and priorities for treatment; and (3) initial and ongoing decision making. Clients focused on the context in which the decisions were made, including as they experienced initial feelings of resistance, and then acceptance of efforts made to describe and treat their mental health challenges. Clients highlighted the need for collaboration between themselves and their clinician, and the need to be equipped with the knowledge and tools to take care of themselves. These focus group data were used to refine the online decision aid. Pilot testing revealed that while it was overall useful and relevant, important limitations were noted by both clients and clinicians.

Discussion: The use of a decision aid to facilitate shared decision making (SDM) in this area is feasible and has utility for both clients and clinicians. Use of such a tool can help to address the need to uphold the rights of young people as decision makers about their own care. Future efforts should embed decision aids within complex SDM interventions, and research to understand issues relating to implementation of these interventions.
Practical Opportunities for Biopsychosocial Education Through Strategic Interprofessional Experiences in Integrated Primary Care
Authors: Funderburk Jennifer S. , Gass Julie , Shepardson Robyn L. , Mitzel Luke D. , Buckheit Katherine A.
Published: September 2021

Even with the expansion of primary care teams to include behavioral health and other providers from a range of disciplines, providers are regularly challenged to deliver care that adequately addresses the complex array of biopsychosocial factors underlying the patient's presenting concern. The limits of expertise, the ever-changing shifts in evidence-based practices, and the difficulties of interprofessional teamwork contribute to the challenge. In this article, we discuss the opportunity to leverage the interprofessional team-based care activities within integrated primary care settings as interactive educational opportunities to build competencies in biopsychosocial care among primary care team members. We argue that this approach to learning while providing direct patient care not only facilitates new provider knowledge and skills, but also provides a venue to enhance team processes that are key to delivering integrated biopsychosocial care to patients. We provide three case examples of how to utilize strategic planning within specific team-based care activities common in integrated primary care settings—shared medical appointments, conjoint appointments, and team huddles—to facilitate educational objectives.
Teaching Multiple Inverse Reinforcement Learners
Authors: Melo Francisco S. , Lopes Manuel
Published: September 2021

In this paper, we propose the first machine teaching algorithm for multiple inverse reinforcement learners. As our initial contribution, we formalize the problem of optimally teaching a sequential task to a heterogeneous class of learners. We then contribute a theoretical analysis of such problem, identifying conditions under which it is possible to conduct such teaching using the same demonstration for all learners. Our analysis shows that, contrary to other teaching problems, teaching a sequential task to a heterogeneous class of learners with a single demonstration may not be possible, as the differences between individual agents increase. We then contribute two algorithms that address the main difficulties identified by our theoretical analysis. The first algorithm, which we dub SplitTeach, starts by teaching the class as a whole until all students have learned all that they can learn as a group; it then teaches each student individually, ensuring that all students are able to perfectly acquire the target task. The second approach, which we dub JointTeach, selects a single demonstration to be provided to the whole class so that all students learn the target task as well as a single demonstration allows. While SplitTeachensures optimal teaching at the cost of a bigger teaching effort, JointTeachensures minimal effort, although the learners are not guaranteed to perfectly recover the target task. We conclude by illustrating our methods in several simulation domains. The simulation results agree with our theoretical findings, showcasing that indeed class teaching is not possible in the presence of heterogeneous students. At the same time, they also illustrate the main properties of our proposed algorithms: in all domains, SplitTeachguarantees perfect teaching and, in terms of teaching effort, is always at least as good as individualized teaching (often better); on the other hand, JointTeachattains minimal teaching effort in all domains, even if sometimes it compromises the teaching performance.
Cod’s Journey Back Home—From Birth to Nursery
Authors: Endo Clarissa Akemi Kajiya , Vikebø Frode B. , Yaragina Natalia A. , Hjøllo Solfrid Sætre , Stige Leif Christian
Published: September 2021

Adult cod swim hundreds of kilometers away from home to release their eggs into the ocean water. After some days, tiny larvae hatch from the eggs. At first, the larvae have a small food reserve to sustain them during their first days of life. Soon after emptying the yolk-sac, the larvae must find food on their own. Both eggs and larvae are carried by the ocean currents and they experience large changes in conditions as they drift back to the nursery areas where their parents came from, where the larvae grow up to become adult fish. Our research on cod indicates that the number and location of cod larvae are associated with the size of the spawning adults; and that the number of larvae influences how many cod will grow to be recruits. So, it is important to also know the living conditions of the parents before they spawn, which will be important for the survival of their offspring.
Physical Activity and Health Promotion in Esports and Gaming–Discussing Unique Opportunities for an Unprecedented Cultural Phenomenon
Authors: Ketelhut Sascha , Martin-Niedecken Anna Lisa , Zimmermann Patrick , Nigg Claudio R.
Published: September 2021

Due to long periods of sedentary behavior, and unhealthy diets gamers and esports players are at risk for numerous chronic diseases and all-cause mortality. Health research has started addressing the public health implications of the esports phenomenon, drawing a bleak picture of this megatrend. However, instead of just highlighting adverse public health implications of this trend, it is recommended to become involved in this phenomenon and positively influence it. Esports has an enormous potential for physical activity and health-promoting efforts, provides a context for broadly disseminating interventions, and offers new ways of gaining access to an often-neglected population. This paper presents: the potential of the esports phenomenon to promote physical activity, health, and well-being in gamers and esports players; the strategic and preventive solutions to ameliorate esports possible adverse health impacts; and the utilization of esports technology (streams, media platforms, exergames, etc.) as an innovative health promotion tool, especially reaching gamers and esports players with attractive and interactive interventions. This is to encourage systematic scientific research so that evidence-based guidelines and intervention strategies involving regular physical activity, healthy diet, and sleep hygiene for esports will be developed. The goal is to promote public health approaches that move toward a better integration of esports and gaming.
Attempt of Real-Time Near-Infrared Fluorescence Imaging Using Indocyanine Green (ICG) in Radical Resection of Gallbladder Cancer: A Case Report
Authors: Yu Yang , Xiang Lin , Bai Yuping , Maswikiti Ewetse Paul , Gu Baohong , Li Xuemei , Li Haiyuan , Zheng Peng , Zhang Ying , Chen Hao
Published: September 2021

Surgery is the mainstay of treatment for resectable gallbladder cancer. Near-infrared fluorescence (NIRF) imaging using ICG is an innovation in laparoscopic surgery, which can provide real-time navigation during the whole operation. In this article, we present a 56-year older woman with gallbladder cancer, in which we evaluated the applicability of NIRF imaging using ICG for tumor and biliary tree visualization during the operative procedure of gallbladder cancer. The tumor and biliary tree were clearly visualized by utilizing a green fluorescence dye. The patient was successfully operated radical resection of gallbladder cancer under fluorescence laparoscope, without any complications. According to this case, the utilization of ICG based NIRF imaging is feasible and beneficial in identifying tumors and the biliary tree during radical resection. It can assist in the achievement of a negative margin and lymphatic clearance around the biliary tree. However, further studies are needed to corroborate the results of this case.
Prevalence and Risk Factors of Hernia in Patients With Rectus Abdominis Diastasis: A 10-Year Multicenter Retrospective Study
Authors: Yuan Sue , Wang Honghong , Zhou Jie
Published: September 2021

Objectives: Hernias are very common in patients with rectus abdominis diastasis (RAD). This study aimed to identify and compare the risk factors and patterns of hernia between men and women with RAD.

Method: We included patients with RAD from six hospitals within the Partners Healthcare System in Massachusetts, USA between 2009 and 2018. Univariate and multivariable binary logistic regression analyses were used to identify risk factors associated with hernia.

Results: Of the 1,294 RAD cases, 866 (67%) were women. The risk of RAD in women was 1.9 times greater than that of men. There were 240 men (56.1%) and 310 women (35.8%) having one or more hernia (P < 0.001). Of the 550 hernia cases, 278 men and 175 women had umbilical hernia (28.1 vs. 38.3%, P = 0.085). The distribution of hernia type differed between the two groups (P < 0.0001). Multivariate analysis identified that alcohol use [odd ratio (OR) 1.74 (1.17–2.59); P = 0.006] and depressive disorder [OR 1.90 (1.209–2.998); P = 0.005] were risk factors of coexisting hernia for men with RAD; age [OR 1.51 (1.33–1.72); P = 0.000] and smoking/tobacco use [OR 1.66 (1.13–2.44); P = 0.010] were risk factors of hernia for women.

Conclusion: The prevalence and risk factors of hernia in women with RAD significantly differed from that in men with RAD. Umbilical hernia is an important type of hernia. Alcohol use and depressive disorder in men, and age and smoking in women were risk factors of hernias in patients with RAD.