bluuebunny/crossref_metadata_2025 huggingface dataset:
Identification of two heterogeneous subtypes of hepatocellular carcinoma with distinct pathway activities and clinical outcomes based on gene set variation analysis
Authors: Jin Zhipeng , Wang Xin , Zhang Xue , Cheng Siqi , Liu Yefu
Published: September 2024

Background

High heterogeneity is an essential feature of malignant tumors. This study aims to reveal the drivers of hepatocellular carcinoma heterogeneity for prognostic stratification and to guide individualized treatment.

Methods

Omics data and clinical data for two HCC cohorts were derived from the Cancer Genome Atlas (TCGA) and the International Cancer Genome Atlas (ICGC), respectively. CNV data and methylation data were downloaded from the GSCA database. GSVA was used to estimate the transcriptional activity of KEGG pathways, and consensus clustering was used to categorize the HCC samples. The pRRophetic package was used to predict the sensitivity of samples to anticancer drugs. TIMER, MCPcounter, quanTIseq, and TIDE algorithms were used to assess the components of TME. LASSO and COX analyses were used to establish a prognostic gene signature. The biological role played by genes in HCC cells was confirmed by in vitro experiments.

Results

We classified HCC tissues into two categories based on the activity of prognostic pathways. Among them, the transcriptional profile of cluster A HCC is similar to that of normal tissue, dominated by cancer-suppressive metabolic pathways, and has a better prognosis. In contrast, cluster B HCC is dominated by high proliferative activity and has significant genetic heterogeneity. Meanwhile, cluster B HCC is often poorly differentiated, has a high rate of serum AFP positivity, is prone to microvascular invasion, and has shorter overall survival. In addition, we found that mutations, copy number variations, and aberrant methylation were also crucial drivers of the differences in heterogeneity between the two HCC subtypes. Meanwhile, the TME of the two HCC subtypes is also significantly different, which offers the possibility of precision immunotherapy for HCC patients. Finally, based on the prognostic value of molecular subtypes, we developed a gene signature that could accurately predict patients’ OS. The riskscore quantified by the signature could evaluate the heterogeneity of HCC and guide clinical treatment. Finally, we confirmed through in vitro experiments that RFPL4B could promote the progression of Huh7 cells.

Conclusion

The molecular subtypes we identified effectively exposed the heterogeneity of HCC, which is important for discovering new effective therapeutic targets.
Sustainable ocean development policies in Indonesia: paving the pathways towards a maritime destiny
Authors: Wuwung Lucky , McIlgorm Alistair , Voyer Michelle
Published: September 2024

Maritime countries, including Indonesia, have indicated their interest in developing a national ocean policy and blue economy plan to boost economic growth while promoting sustainability in and from oceanic activities. In 2017, the Government of Indonesia published the Indonesian Ocean Policy (IOP), the first of its kind since independence, and subsequently developed a series of blue economy documents as an integral part of its national development agenda whilst also indicating its support for the global sustainability objectives stipulated in the 2015 Sustainable Development Goals (SDGs). However, development obstacles such as: declining ocean health; climate crises; fragmented ocean management; inadequate infrastructure and technology; limited data support; low sustainable investment; and the blurred definition of the blue economy, pose risks to successful outcomes of sustainable ocean development in Indonesia. When unaddressed, these challenges can inhibit the policy’s effectiveness in delivering its intended outcomes. This qualitative study explores Indonesia’s interests in sustainable ocean development and how it pursues them while advancing economic objectives and commitments towards SDGs and net zero emission targets. The study finds that maintaining ocean health and ensuring sustainable use of ocean resources are Indonesia’s most vital interests for sustainable ocean development. The national ocean policy and the blue economy have been employed as two successive and simultaneous avenues to pursue Indonesia’s maritime interests. Unfortunately, to date, both approaches have yet to obtain maturity and ocean affairs are overshadowed by other national development priorities. By focusing on ocean governance and its development in the contemporary context, this article sheds light on the important role of policymaking in modern-day ocean governance. A cautious approach to policy design is crucial to avoid common pitfalls in sustainable ocean development, such as weak implementation strategies and the consequent failure to meet core sustainability objectives.
Factors influencing mangrove carbon storage and its response to environmental stress
Authors: Guo Chuanyi , Loh Pei Sun , Hu Jianxiong , Chen Zengxuan , Pradit Siriporn , Oeurng Chantha , Sok Ty , Mohamed Che Abd Rahim , Lee Choon Weng , Bong Chui Wei , Lu Xixi , Anshari Gusti Z. , Kandasamy Selvaraj , Wang Jianjun
Published: September 2024

Mangrove forests serve as significant carbon sinks and play a crucial role in mitigating climate change. Currently, the response of mangroves to intensified climate change and human activities, and the factors that influence the magnitude of carbon storage in their sediments remain uncertain. To address these questions, two sediment cores were collected from the mangrove reserve in Pearl Bay, Guangxi, China. The activity of 210Pb in the sediment, grain size, bulk elemental composition, stable carbon isotopes, lignin, and different sediment organic matter (OM) fractions were investigated to determine the local mangrove’s response to climate change and human activities, as well as the factors influencing its carbon storage. The results showed mangrove forests with lower tidal ranges, slower sedimentation rates, and where OM predominantly originated locally tend to have larger carbon stocks. The mangrove OM (MOM) decreased progressively from the bottom to the top of the cores, indicating that the mangroves in Pearl Bay have possibly undergone degradation, which was further substantiated by the decrease in lignin content. Based on these results, the entire cores were divided into two stages: stable stage 1 (1963–2001) and degradation stage 2 (2001–2020). The cause of the mangrove degradation is likely due to the impact of human activities; however, these impacts are anticipated to gradually lessen in the future due to mangrove protection policies. Our results indicate that lignin can track and predict mangrove growth trends and provide guidance for the sustainable management of mangrove ecosystems.
Characteristics and real-world medication persistence of people living with HIV treated with DTG/3TC or BIC/FTC/TAF: a hospital claims database study in Japan
Authors: Kanamori Rie , Aoki Nozomi , Kanazawa Akio , Yuda Mayumi , Makino Nao , Ohata Emi , Fukui Nobuyuki , Mori Hirotake , Yokokawa Hirohide , Naito Toshio
Published: September 2024

Background

As the life expectancy of people living with human immunodeficiency virus (HIV) (PLWH) has improved, chronic disease burden and polypharmacy have increased in PLWH. Simplification of the antiretroviral therapy (ART) regimen for PLWH has become crucial. The real-world treatment patterns and medication persistence of the 2-drug single-tablet regimen (STR), dolutegravir/lamivudine (DTG/3TC), compared to bictegravir/emtricitabine/tenofovir alafenamide (BIC/FTC/TAF) prescribed were investigated.

Methods

This retrospective, database study extracted data from a hospital-based medical claims database in Japan. The changes in ART distributions by year during the identification period between January 1, 2018 and December 31, 2021 were observed. Patients with disease record of HIV-1 infection and prescribed DTG/3TC or BIC/FTC/TAF as the first prescription of STR during the identification period were divided into two cohorts; DTG/3TC cohort and BIC/FTC/TAF cohort, respectively. Patient without medication records more than 3 months and no future data more than 6 months were excluded. Patients’ characteristics were compared between the DTG/3TC cohort and the BIC/FTC/TAF cohort by Mantel–Haenszel test to adjust for age. Medication persistence was compared between the two cohorts by evaluating the continuation rates using Kaplan–Meier methods, using the log-rank test to assess the difference between the Kaplan–Meier curves. The median time-to-first prescription was compared between the two cohorts by Kaplan–Meier methods.

Results

Prescriptions of DTG/3TC and BIC/FTC/TAF increased steadily from 2019 to 2021 after the release year of each STR. There was no significant difference in the time-to-first prescription (p = 0.3). A total of 959 patients were included, with 120 patients and 839 patients on DTG/3TC and BIC/FTC/TAF, respectively. The proportion of dyslipidemia at baseline was significantly higher in the DTG/3TC cohort than in the BIC/FTC/TAF cohort after adjusting for mean age (p = 0.002). There was no significant difference in medication persistence between the two cohorts (p = 0.91).

Conclusion

This study showed that DTG/3TC was likely to be selected for elderly patients and those with chronic disease in real-world clinical practice, which seems in accordance with the treatment strategy recommended by guidelines. Comparable medication persistence was observed with both regimens, aligning with findings from other countries. The 2-drug single-tablet regimen DTG/3TC may be an important ART regimen for PLWH with multiple morbidities and polypharmacy in an aging society. Due to the limitations of the database, further research to assess viral loads, emergence of resistance and adverse events will be encouraged.
Endoplasmic reticulum stress response pathway-mediated cell death in ovarian cancer
Authors: Chen Qiaochu , Li Chan , Wei Wei , Li Jia , Liu Fangyuan , Fu Yuqian , Tang Liping , Han Fengjuan
Published: September 2024

The endoplasmic reticulum (ER) is one of the largest organelles, and Endoplasmic Reticulum Stress Response Pathway is a series of responses triggered by the homeostatic imbalance of the ER and the state in which unfolded or misfolded proteins accumulate in the ER, which can trigger cell death. Cell death plays a crucial role in the development of diseases such as gynecological oncology. Herein, we review the current research on the response and ovarian cancer, discussing the key sensors (IRE1, PERK, ATF6), and the conditions under which it occurs (Ca2+ homeostasis disruption, hypoxia, others). Using the response as a starting point, provide a comprehensive overview of the relationship with the four types of cell death (apoptosis, autophagy, immunogenic cell death, paraptosis) in an attempt to provide new targeted therapeutic strategies for the organelle-Endoplasmic Reticulum Stress Response Pathway-cell death in ovarian cancer therapy.
Investigating the molecular mechanisms of Fuzheng Yiliu Shenji prescription in SH-SY5Y neuroblastoma cells
Authors: Zhu Xueying , Si Yinchu , Gai Cong , Li Zhong
Published: September 2024

Background

Neuroblastoma is the most common extracranial solid tumor in childhood. Fuzheng Yiliu Shenji Prescription (FYSP) has shown potential in treating malignant pediatric tumors in clinical settings. This study aims to explore the molecular mechanisms behind its effects, specifically in the context of neuroblastoma cell lines.

Objective

To elucidate the active compounds in FYSP and their mechanisms of action in inhibiting neuroblastoma cell viability, inducing apoptosis, and affecting the cell cycle in SH-SY5Y cells through network pharmacology and empirical validation.

Materials and methods

We identified the major compounds in FYSP and their predicted targets, constructing a protein-protein interaction (PPI) network and performing GO and KEGG pathway analyses. The effects of FYSP were empirically validated through assays on cell viability, cell cycle, apoptosis, and protein expression in SH-SY5Y cells.

Results

The study identified 172 active chemical components in FYSP, with 188 common targets related to neuroblastoma. Network analysis highlighted the PI3K-Akt pathway as a significant target. Experimental validation in SH-SY5Y cells confirmed that FYSP could inhibit cell viability, induce G2/M cell cycle arrest, and promote apoptosis through modulation of the PI3K-Akt pathway, specifically upregulating caspase-3 and downregulating Bcl-2/Bax expression.

Conclusion

The study elucidates the molecular basis of FYSP’s effects on neuroblastoma cells in vitro, demonstrating its ability to modulate key pathways involved in cell cycle and apoptosis. While these findings suggest a potential therapeutic role for FYSP, they are limited to in vitro observations, and further research, including in vivo studies, is necessary to explore its clinical applicability.
Discovery of key biomarkers in tourette syndrome by network pharmacology
Authors: Zhao Jiali , Bai Xiaohong
Published: September 2024

Background

Yangxue Xifeng Decoction (YXD) has been utilized in clinical settings for the treatment of Tourette Syndrome (TS). However, the action mechanism of YXD needs further research.

Methods

The ingredients and targets of YXD were identified via database searches and then constructed an active ingredient-target network using Cytoscape. Pathway enrichment analysis was performed via Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). The core genes were determined by LASSO regression and SVM algorithm. Additionally, we analyzed the immune infiltration. The signaling pathways associated with core genes were investigated through KEGG and GO. We predicted the transcription factors using “RcisTarge”.

Results

127 active ingredients of YXD and 255 targets were obtained. TNF and the IL-17 signaling pathway were the main pathways. OPRM1 and VIM were screened out as core genes, which were associated with the immune infiltration. The signaling pathways involved in OPRM1 and VIM were enriched. Furthermore, remarkable correlation was found between OPRM1 and VIM levels and other TS-related genes such as MAPT and MAPT.

Conclusion

OPRM1 and MAPT, and the signaling pathways are associated with TS. YXD exerts its therapeutic TS through multi-component and multi-targets including immune infiltration.
Role of ferroptosis in the pathogenesis of heart disease
Authors: Fatima Sulail , Zhou Haiyan , Chen Yi , Liu Qinghang
Published: September 2024

Ferroptosis is a new form of regulated necrosis characterized by iron-dependent lipid peroxidation, leading to irreparable lipid damage, membrane permeabilization, and necrotic cell death. Ferroptosis has recently been implicated in the pathogenesis of multiple forms of heart disease such as myocardial infarction, cardiac hypertrophy, heart failure, and various cardiomyopathies. Important progress has also been made regarding how ferroptosis is regulated in vitro and in vivo as well as its role in cardiac homeostasis and disease pathogenesis. In this review, we discuss molecular mechanisms that regulates ferroptosis in the heart, including pathways leading to iron overload and lipid peroxidation as well as the roles of key organelles in this process. We also discuss recent findings pertaining to the new pathogenic role of ferroptosis in various forms of heart disease as well as genetic and pharmacologic strategies targeting ferroptosis in the heart.
Revealing genetic causality between blood-based biomarkers and major depression in east Asian ancestry
Authors: Mo Xiaoxiao , Wang Chao , Pu Qiuyi , Zhang Zhengdong , Wu Dongmei
Published: September 2024

Introduction

Major Depression (MD) is a common mental disorder. In East Asian ancestry, the association, causality, and shared genetic basis between blood-based biomarkers and MD remain unclear.

Methods

We investigated the relationships between blood-based biomarkers and MD through a cross-sectional study and Mendelian randomization (MR) analysis. Cross-trait analysis and enrichment analyses were used to highlight the shared genetic determinants and biological pathways. We conducted summary data–based MR to identify shared genes, which were then validated using a transcriptome dataset from drug-naïve patients with MD.

Results

In the cross-sectional study, C-Reactive Protein showed the significantly positive correlation with depressive symptoms, while hematocrit, hemoglobin, and uric acid exhibited significantly negative correlations. In MR analysis, basophil count (BASO) and low-density lipoprotein cholesterol (LDLc) had a significant causal effect on MD. The enrichment analysis indicated a significant role of inflammatory cytokines and oxidative stress. The shared genes MFN2, FAM55C, GCC2, and SCAPER were validated, with MFN2 identified as a pleiotropic gene involved in MD, BASO, and LDLc.

Discussion

This study highlighted that BASO and LDLc have a causal effect on MD in East Asian ancestry. The pathological mechanisms of MD are related not only to inflammatory cytokines and oxidative stress but also to down regulation of MFN2 expression and mitochondrial dysfunction.
Evaluation of a pre-post quasi-experimental educational intervention on breast cancer awareness among pharmacy professionals in Karachi, Pakistan
Authors: Mansoor Noor us Saba , Naveed Safila , Ali Huma , Manzoor Ammara , Zahoor Saima , Sheikh Javeria
Published: September 2024

Introduction

Cancer, particularly breast cancer, is a major contributor to mortality and a significant impediment to life expectancy. In 2020, breast cancer accounted for 11.7% of all cancer cases and caused approximately 685,000 deaths worldwide, surpassing lung cancer in prevalence. The study aims to evaluate the impact of an educational intervention on breast cancer awareness among pharmacy students by comparing their understanding before and after the program.

Method

A pre-post quasi-experimental study was designed to assess knowledge and awareness of breast cancer, breast self-examination (BSE) practices, and attitudes toward breast cancer among female university students in Karachi, Pakistan. Participants completed a pre-session questionnaire, attended an awareness workshop and video tutorial, and then completed a post-session questionnaire 2 weeks later.

Results

Of 1,200 participants, 1,015 of them completed both the pre- and post-intervention questionnaires. Key demographic features included 83.9% of the participants being in the 18–24 age group, 26.8% being married, and only 14.2% having a family history of breast cancer. Before the intervention, 60.7% of the participants were not involved in regular breast self-exams due to a lack of awareness. Post-intervention results showed a significant increase in awareness, with 35.9% rising to 94.9%. The use of screening methods increased from 46.7 to 94.8%. Knowledge of breast cancer improved from 51.2 to 96.7%, and the general perception rose from 48.2 to 93.4%. Attitudes toward self-examination also shifted positively, indicating a significant change in perception.

Interpretation and conclusion

The study concludes the baseline knowledge of breast cancer among female students was inadequate but improved significantly from over 40% to over 90% following the educational intervention. The intervention positively influenced the general perception and attitudes toward breast cancer. These findings highlight the need for regular educational sessions to enhance awareness, improve healthcare outcomes, and reduce mortality rates, particularly in developing countries.
The tumor microenvironment in therapy resistance
Authors: Garcia Guadalupe G. , Schmidt Christopher J. , Hajal Cynthia
Published: September 2024

Chemotherapy alone or in conjunction with surgery and radiation is often used to treat various cancer types. While effective at treating some tumors, the response varies across patients with different malignancies. For some cancers, such as glioblastoma, ovarian cancer, and soft tissue sarcoma, 85%–100% of patients experience cancer recurrence and develop chemotherapy resistance, which often leads to worse prognoses. These alarming statistics highlight an urgent need to better understand the landscape of therapy resistance in cancer, in order to develop improved treatment strategies and prevent recurrence. A central focus has been the investigation of resistant tumor subclones and whether the use of different alkylating agents and/or immune checkpoint inhibitors can ablate different clones. However, very little effort has been directed towards studies of the tumor microenvironment, a complex ecosystem of blood vessels, fibroblasts, immune cells, signaling molecules, and extracellular matrix, in the context of therapy resistance. In this perspective, we provide an overview of different platforms, tools, and techniques that have been developed and used to identify tumor microenvironment alterations due to therapy resistance. We also address potential therapeutic strategies that involve components of the tumor milieu and have been identified and tested to overcome treatment-induced resistance. Identifying microenvironmental changes post-resistance presents opportunities for new targeted treatment strategies. The current state of the literature suggests a dire need for more engineered models that probe specific microenvironment contributors to therapy resistance or ways in which the tumor tissue can be harnessed to mitigate resistance.
Omnidirectional soft pneumatic actuators: a design and optimization framework
Authors: Moutousi Maria , Polygerinos Panagiotis
Published: September 2024

Introduction

Soft pneumatic actuators (SPAs) play a pivotal role in soft robotics due to their unique characteristics of compliance, flexibility, and adaptability. There are plenty of approaches that examine the modeling parameters of SPAs, aiming to optimize their design and, thus, achieve the most advantageous responses. Current optimization methods applied to SPAs are usually performed individually for each design parameter without considering the simultaneous effect all parameters can have on the output performance. This modeling shortcoming is essential to be addressed since customized SPAs are used in a variety of applications, each with different output requirements.

Methods

This study provides a generalized design optimization framework for modeling the SPA performance for their motion profiles, the produced strain energy while being deformed, and their stiffness characteristics. Utilizing experimentally validated finite element methods, all geometrical and material parameters of the models are investigated in response surface methodology optimization using the central composite design approach.

Results

The results showcase the entire design space of omnidirectional SPAs, along with their output performance, providing guidelines to the end user for design optimization.

Discussion

The offering of this modeling process for SPAs can be adapted to the demands of any potential application and ensure the best performance with respect to the required output responses.
A study on the grey relation analysis between the overall performance and individual event scores of international elite trampolinists
Authors: Zhu Xinyu , Wang Lejun
Published: September 2024

This study applied grey relational analysis to assess the relationship between individual trampoline event scores and overall performance of top male and female athletes in the 2020 Tokyo Olympics. Analyzing execution, horizontal, difficulty, timing of flight, and total scores, results showed males excelled significantly in difficulty, timing, and overall performance, while execution and horizontal scores were comparable. For males, timing of flight (excluding outliers) had the greatest influence on total score, followed by difficulty and execution. In females, difficulty dominated, followed by timing and horizontal, with execution least impactful. The study highlighted the primary roles of timing and difficulty scores in overall trampoline performance, with gender variations in score contributions. The findings illuminated the interplay of score components, offering a theoretical framework for targeted trampoline training. For international athletes, key considerations included boosting height index for a robust trampoline foundation; tailoring difficulty levels to athletes' abilities while adhering to scoring rules, without sacrificing technical prowess; and sustaining training to refine quality and stability of routines.
Efficiency of Backwashing in Removing Solids from Sand Media Filters for Drip Irrigation Systems
Authors: Duran-Ros Miquel , Pujol Joan , Pujol Toni , Cufí Sílvia , Graciano-Uribe Jonathan , Arbat Gerard , Ramírez de Cartagena Francisco , Puig-Bargués Jaume
Published: September 2024

Sand media filters are especially recommended to prevent emitter clogging with loaded irrigation waters, but their performances rely on backwashing. Despite backwashing being a basic procedure needed to restore the initial filtration capacity, there is a lack of information about the solid removal efficiency along the media bed depth. An experimental filter with a 200 mm silica sand bed height was used to assess the effect of two operation velocities (30/45 and 60/75 (filtration/backwashing) m h−1) and two clogging particles (inorganic sand dust and organic from a reclaimed effluent) on the efficiency of backwashing for removing the total suspended solids retained in different media bed slices. The average solid removal backwashing efficiency was greater with organic particles (78%) than with inorganic ones (64%), reaching its maximum at a 5–15 mm bed depth. A higher operation velocity increased the solid removal efficiency by 16%, using organic particles, but no significant differences were observed with inorganic particles. The removal efficiencies across the media bed were more uniform with organic particles (63–89%) than with inorganic (40–85%), which makes it not advisable to reduce the media height when reclaimed effluents are used. This study may contribute to future improvements in sand media filter design and management.
Impact of Dolomite Liming on Ammonia-Oxidizing Microbial Populations and Soil Biochemistry in Acidic Rice Paddy Soils
Authors: Shaaban Muhammad , Wang Xiaoling , Song Peng , Hu Ronggui , Wu Yupeng
Published: September 2024

Background and Aims: Over the last few decades, rampant nitrogen fertilization has exacerbated soil acidification in agricultural ecosystems. To counteract this, liming has become an essential technique for rehabilitating fertility in acid-degraded agricultural soils. Our research aimed to shed light on the response of ammonia oxidizers to liming in acidic soils within a controlled rice paddy experiment. We conducted a pot experiment with rice, featuring three different treatments: a control with only soil, a low dolomite dose (LD), and a high dolomite dose (HD). Various soil properties were investigated throughout the study. Under flooding, soil pH values rose across the treatments, from 5.4 in the control to 6.8 in HD. Ammonium and nitrate levels peaked in the HD treatment, reaching 30 and 22 mg kg−1, respectively. Similarly, dissolved organic carbon and microbial biomass carbon surged at mid-season aeration, hitting highs of 101 and 30 mg kg−1, respectively, in the HD treatment. Ammonia-oxidizing bacteria (AOB) and archaea (AOA) were responsive to dolomite-lime application, with distinct reactions; AOB abundance and potential nitrification rates were positively affected by higher lime doses, whereas AOA numbers decreased over time and with dolomite application. Additionally, soil enzymes such as urease, catalase, invertase, phenol oxidase, and phosphatase also increased progressively, mirroring the rise in soil pH. This study identified increased soil pH as the critical factor influencing various soil parameters, especially the balance between AOA and AOB populations. Both AOB and AOA were sensitive to liming; AOA decreased while liming stimulated AOB abundance.
Efficacy and Safety of Antibiotics in the Treatment of Methicillin-Resistant Staphylococcus aureus (MRSA) Infections: A Systematic Review and Network Meta-Analysis
Authors: Liu Qi , He Dongxia , Wang Lei , Wu Yuewei , Liu Xian , Yang Yahan , Chen Zhizhi , Dong Zhan , Luo Ying , Song Yuzhu
Published: September 2024

Background: Vancomycin is a first-line drug for the treatment of MRSA infection. However, overuse of vancomycin can cause bacteria to become resistant, forming resistant strains and making infections more difficult to treat. This study aimed to evaluate the efficacy and safety of different antibiotics in the treatment of MRSA infections and to compare them, mainly with vancomycin, to find better vancomycin alternatives. Methods: All studies were obtained from the PubMed and Embase databases from inception to 13 April 2023. The three comprehensive indicators of clinical cure success rate, clinical microbiological success rate, and adverse reactions were evaluated, and the clinical cure success rates of three disease types, complex skin and skin structure infections (cSSSIs), complex skin and soft tissue infections (cSSTIs), and pneumonia, were analyzed in subgroups. All statistical analyses were performed using R and STATA 14.0 software for network meta-analysis. Results: A total of 38 trials with 6281 patients were included, and 13 drug treatments were evaluated. For MRSA infections, the results of network meta-analysis showed that the clinical success rates of linezolid, the combination of vancomycin and rifampin, and the combination of minocycline and rifampin were better than that of vancomycin (RR 1.71; 95%-CI 1.45–2.02), (RR 2.46; 95%-CI 1.10–5.49) (RR, 2.77; 95%-CI 1.06–7.21). The success rate of clinical microbiological treatment with vancomycin was inferior to that with telavancin (RR 0.74; 95%-CI 0.55–0.99). Linezolid had a higher rate of adverse reactions than teicoplanin (RR 5.35; 95%-CI 1.10–25.98). Subgroup analysis showed that vancomycin had a lower clinical success rate than linezolid in the treatment of MRSA-induced cSSSIs, cSSTIs, and pneumonia (RR 0.59; 95%-CI 0.44–0.80) (RR 0.55; 95%-CI 0.35–0.89) (RR 0.55; 95%-CI 0.32–0.93). Conclusions: This systematic review and NMA provide a new comparison framework for the clinical treatment of MRSA infection. The NMA suggests that linezolid may be the antibiotic of choice for the treatment of MRSA infections, with the ability to improve clinical and microbiological success rates despite its disadvantage in terms of adverse effects. At the same time, the combination of minocycline and rifampicin may be the most effective drug to treat MRSA-induced cSSSIs, tedizolid may be the best drug to treat MRSA-induced cSSTIs, and the combination of vancomycin and rifampicin may be the most effective treatment for MRSA-induced pneumonia. More high-quality studies are still needed in the future to further identify alternatives to vancomycin. Trial registration: PROSPERO registration number CRD42023416788.
Effect of Combination of Point-of-Care C-Reactive Protein Testing and General Practitioner Education and Long-Term Effect of Education on Reducing Antibiotic Prescribing for Children Presenting with Acute Infections in General Practice in Latvia: A Randomized Controlled Intervention Study
Authors: Likopa Zane , Kivite-Urtane Anda , Strele Ieva , Pavare Jana
Published: September 2024

Background: Antibiotics are often overprescribed in children in general practice. We investigated whether the availability of C-reactive protein point-of-care testing (CRP POCT) in daily practice and general practitioner (GP) education reduces antibiotic prescribing for children with acute infections and whether GP education has a long-term effect on antibiotic prescribing. Methods: This was a randomized controlled intervention study with randomization at the GP practice level. Eligible patients were children aged 1 month to 17 years presenting to general practice with an acute infection. Interventions: In the first study period, one GP group received combined interventions (CRP POCT was provided for daily use in combination with a live educational session), while the second GP group continued usual care. During the second study period, the GP groups were switched. During this period, the long-term education effect was evaluated in the GP group, which had previously received both interventions: the CRP POCT was no longer available in their practices in accordance with the study protocol, but education could have a lasting effect. Primary outcome: Antibiotic prescribing at index consultation. Results: GP with combined intervention enrolled 1784 patients, GP with usual care enrolled 886 patients, and GP with long-term education effect enrolled 647 patients. Most of the patients had upper (76.8%) and lower (18.8%) respiratory infections. In total, 29.3% of the study patients received antibiotic prescriptions. Adjusted binary logistic regression analysis showed no differences for the primary outcome between GPs with usual care and GPs with combined intervention (aOR 0.89 (0.74–1.07), p = 0.20), but significantly lower antibiotic prescribing was observed for GPs with long-term education in comparison with GPs with usual care (aOR 0.75 (0.59–0.96), p = 0.02); however, after multilevel analyses, any differences in the antibiotic prescription between intervention groups became non-significant. GPs widely used CRP POCT when it was available in practice (for 69.1% of patients in the combined intervention group), but rarely measured CRP in the laboratory in the usual care group (8.8% (n = 78)) or long-term education group (14.8% (n = 98)). The majority of the tested patients had low CRP levels (below 20 mg/L); despite this, up to 35.4% of them received antibiotic prescriptions. Conclusions: Our results show that the availability of CRP POCT and educational training for GPs together did not reduce antibiotic prescribing, and one-time education did not have a long-term effect on antibiotic prescribing.
Improving the Extraction of Polyphenols from Cocoa Bean Shells by Ultrasound and Microwaves: A Comparative Study
Authors: Disca Vincenzo , Travaglia Fabiano , Carini Chiara , Coïsson Jean Daniel , Cravotto Giancarlo , Arlorio Marco , Locatelli Monica
Published: September 2024

The extraction of bioactive compounds from food by-products is one of the most important research areas for the nutraceutical, pharmaceutical, and food industries. This research aimed to evaluate the efficiency of Ultrasound-Assisted Extraction (UAE) and Microwave-Assisted Extraction (MAE), either alone or in combination, of phenolic compounds from cocoa bean shells (CBSs). These extraction techniques were compared with conventional methods, such as under simple magnetic stirring and the Soxhlet apparatus. After the preliminary characterization of the gross composition of CBSs, the total polyphenol content and radical scavenging of extracts obtained from both raw and defatted cocoa bean shells were investigated. Quantification of the main polyphenolic compounds was then performed by RP-HPLC-DAD, identifying flavonoids and phenolic acids, as well as clovamide. The application of MAE and UAE resulted in a similar or superior extraction of polyphenols when compared with traditional methods; the concentration of individual polyphenols was variously influenced by the extraction methods employed. Combining MAE and UAE at 90 °C yielded the highest antiradical activity of the extract. Spectrophotometric analysis confirmed the presence of high-molecular-weight melanoidins, which were present in higher concentrations in the extracts obtained using MAE and UAE, especially starting from raw material. In conclusion, these results emphasize the efficiency of MAE and UAE techniques in obtaining polyphenol-rich extracts from CBS and confirm this cocoa by-product as a valuable biomass for the recovery of antioxidant compounds, with a view to possible industrial scale-up.
Multi-Sensor Platform in Precision Livestock Farming for Air Quality Measurement Based on Open-Source Tools
Authors: Danev Victor , Atanasova Tatiana , Dineva Kristina
Published: September 2024

Monitoring air quality in livestock farming facilities is crucial for ensuring the health and well-being of both animals and workers. As livestock farming can contribute to the emission of various gaseous and particulate pollutants, there is a pressing need for advanced air quality monitoring systems to manage and mitigate these emissions effectively. This study introduces a multi-sensor air quality monitoring system designed specifically for livestock farming environments. Utilizing open-source tools and low-cost sensors, the system can measure multiple air quality parameters simultaneously. The system architecture is based on SOLID principles to ensure robustness, scalability, and ease of maintenance. Understanding a trend of evolution of air quality monitoring from single-parameter measurements to a more holistic approach through the integration of multiple sensors, a multi-sensor platform is proposed in this work. This shift towards multi-sensor systems is driven by the recognition that a comprehensive understanding of air quality requires consideration of diverse pollutants and environmental factors. The aim of this study is to construct a multi-sensor air quality monitoring system with the use of open-source tools and low-cost sensors as a tool for Precision Livestock Farming (PLF). Analysis of the data collected by the multi-sensor device reveals some insights into the environmental conditions in the monitored barn. Time-series and correlation analyses revealed significant interactions between key environmental parameters, such as strong positive correlations between ammonia and hydrogen sulfide, and between total volatile organic compounds and carbon dioxide. These relationships highlight the critical impact of these odorants on air quality, emphasizing the need for effective barn environmental controls to manage these factors.
The Ideal Strategy of Carbon-Neutral for Park Landscape Design: A Proposal for a Rapid Detection Method
Authors: Ou Shengjung , Chien Yuchen , Hsu Cheyu , Ning Fuer , Pan Haozhang
Published: September 2024

The primary objective of this study is to investigate the carbon footprint, resilience levels, and optimal landscape area ratios of various parks. Additionally, it explores the relationships between landscape element proportions (LEP), the normalized difference vegetation index (NDVI), resilience indicators (RI), and the carbon reduction benefits associated with carbon neutrality (CN). Six parks were assessed for resilience, NDVI, LEP, and CN values, with Pearson correlation analysis conducted. The results revealed that parks with or without waterbodies exhibited ideal LEP area ratios of 6.5:2:1.5 (Softscape:Waterbody:Hardscape) and 8.3:1.7 (Softscape:Hardscape), respectively. Enhanced Softscape and reduced Hardscape proportions in parks correlated with increased NDVI and CN. NDVI exhibited a positive correlation with Softscape percentage and a negative correlation with Hardscape percentage. Conversely, CN demonstrated a negative correlation with Hardscape percentage and a positive correlation with Softscape percentage. Suggesting Softscape should constitute over 65%, and Hardscape should be under 15% in parks with water bodies. Waterless parks are advised to maintain a Softscape ratio exceeding 83% and a Hardscape ratio below 17%. Finally, the study extended to assess the LEP of 22 additional parks, validating the suitability of the ideal LEP area ratio.
Geospatial and Temporal Patterns of Natural and Man-Made (Technological) Disasters (1900–2024): Insights from Different Socio-Economic and Demographic Perspectives
Authors: Cvetković Vladimir M. , Renner Renate , Aleksova Bojana , Lukić Tin
Published: September 2024

This pioneering study explores the geospatial and temporal patterns of natural and human-induced disasters from 1900 to 2024, providing essential insights into their global distribution and impacts. Significant trends and disparities in disaster occurrences and their widespread consequences are revealed through the utilization of the comprehensive international EM-DAT database. The results showed a dramatic escalation in both natural and man-made (technological) disasters over the decades, with notable surges in the 1991–2000 and 2001–2010 periods. A total of 25,836 disasters were recorded worldwide, of which 69.41% were natural disasters (16,567) and 30.59% were man-made (technological) disasters (9269). The most significant increase in natural disasters occurred from 1961–1970, while man-made (technological) disasters surged substantially from 1981–1990. Seasonal trends reveal that floods peak in January and July, while storms are most frequent in June and October. Droughts and floods are the most devastating in terms of human lives, while storms and earthquakes cause the highest economic losses. The most substantial economic losses were reported during the 2001–2010 period, driven by catastrophic natural disasters in Asia and North America. Also, Asia was highlighted by our research as the most disaster-prone continent, accounting for 41.75% of global events, with 61.89% of these events being natural disasters. Oceania, despite experiencing fewer total disasters, shows a remarkable 91.51% of these as natural disasters. Africa is notable for its high incidence of man-made (technological) disasters, which constitute 43.79% of the continent’s disaster events. Europe, representing 11.96% of total disasters, exhibits a balanced distribution but tends towards natural disasters at 64.54%. Examining specific countries, China, India, and the United States emerged as the countries most frequently affected by both types of disasters. The impact of these disasters has been immense, with economic losses reaching their highest during the decade of 2010–2020, largely due to natural disasters. The human toll has been equally significant, with Asia recording the most fatalities and Africa the most injuries. Pearson’s correlation analysis identified statistically significant links between socioeconomic factors and the effects of disasters. It shows that nations with higher GDP per capita and better governance quality tend to experience fewer disasters and less severe negative consequences. These insights highlight the urgent need for tailored disaster risk management strategies that address the distinct challenges and impacts in various regions. By understanding historical disaster patterns, policymakers and stakeholders can better anticipate and manage future risks, ultimately safeguarding lives and economies.
The Ballpark Effect: Spatial-Data-Driven Insights into Baseball’s Local Economic Impact
Authors: Giri Aviskar , Sagan Vasit , Podgursky Michael
Published: September 2024

The impact of sporting events on local economies and their spatial distribution is a topic of active policy debate. This study adds to the discussion by examining granular cellphone location data to assess the spillover effects of Major League Baseball (MLB) games in a major US city. Focusing on the 2019 season, we explore granular geospatial patterns in mobility and consumer spending on game days versus non-game days in the Saint Louis region. Through density-based clustering and hotspot analysis, we uncover distinct spatiotemporal signatures and variations in visitor affluence across different teams. This study uses features like game day characteristics, location data (latitude and longitude), business types, and spending data. A significant finding is that specific spatial clusters of economic activity are formed around the stadium, particularly on game days, with multiple clusters identified. These clusters reveal a marked increase in spending at businesses such as restaurants, bars, and liquor stores, with revenue surges of up to 38% in certain areas. We identified a significant change in spending patterns in the local economy during games, with results varying greatly across teams. Notably, the XGBoost model performs best, achieving a test R2 of 0.80. The framework presented enhances the literature at the intersection of urban economics, sports analytics, and spatial modeling while providing data-driven actionable insights for businesses and policymakers.
Advanced Nanotechnological Approaches for Biofilm Prevention and Control
Authors: Ferraz Maria Pia
Published: September 2024

Biofilm-associated infections present a significant challenge in modern medicine, primarily due to their resilience and resistance to conventional treatments. These infections occur when bacteria form biofilms, protective layers formed by bacterial communities, which are notoriously resistant to traditional antibiotics on surfaces such as medical implants and biological surfaces, making eradication with standard antibiotics difficult. This resilience leads to persistent infections, imposing a substantial economic burden on healthcare systems. The urgency to find alternative treatments is critical as current methods are insufficient and costly. Innovative approaches, such as nanotechnology-based therapies, offer promising alternatives by targeting biofilms more effectively and reducing the need for invasive procedures. Nanocarriers hold significant promise in the fight against biofilm-associated infections. Nanocarriers can penetrate biofilms more effectively than conventional treatments, delivering higher concentrations of antibiotics or other antimicrobial agents precisely where they are needed. This targeted approach not only enhances the efficacy of treatments but also minimizes potential side effects. The development of nanocarrier-based therapies is crucial for overcoming the limitations of current treatments and ultimately improving patient outcomes and reducing the economic burden of biofilm-associated infections on healthcare systems. In this review, nanotechnology-based systems, their characteristics, limitations, and potential benefits are explored to address biofilms-related infections. Additionally, biofilm evaluation models and the tests necessary for the preclinical validation of these nanosystems to facilitate their clinical application are addressed.