bluuebunny/crossref_metadata_2025 huggingface dataset:
Dietary Supplementation with Epicatechin Improves Intestinal Barrier Integrity in Mice
Authors: Wan Jin , Zhang Li , Ruan Zheng
Published: October 2022

Epicatechin (EPI) is a dietary flavonoid that is present in many foods and possesses various bioactivities. We assessed the effects of EPI supplementation on intestinal barrier integrity in mice. Thirty-six mice were assigned to three groups and fed a standard diet or a standard diet supplemented with 50 or 100 mg EPI/kg (n = 12 per group). After 21 days of rearing, blood and intestinal samples were collected from eight randomly selected mice. Supplementation with 50 and 100 mg/kg EPI decreased (p < 0.05) the serum diamine oxidase activity and D-lactic acid concentration and increased (p < 0.05) the duodenal, jejunal, and ileal abundance of tight junction proteins, such as occludin. Moreover, it lowered (p < 0.05) the duodenal, jejunal, and ileal tumor necrosis factor-α contents and enhanced (p < 0.05) the duodenal and jejunal catalase activities and ileal superoxide dismutase activity. Supplementation with a lower dose (50 mg/kg) decreased (p < 0.05) the ileal interleukin-1β content, whereas supplementation with a higher dose (100 mg/kg) increased (p < 0.05) the duodenal and jejunal glutathione peroxidase activities. Furthermore, supplementation with 50 and 100 mg/kg EPI decreased (p < 0.05) cell apoptosis, cleaved cysteinyl aspartate-specific proteinase-3 (caspase-3), and cleaved caspase-9 contents in the duodenum, jejunum, and ileum. In conclusion, EPI could improve intestinal barrier integrity in mice, thereby suppressing intestinal inflammation and oxidative stress and reducing cell apoptosis.
The Effect of Indole-3-Lactic Acid from Lactiplantibacillus plantarum ZJ316 on Human Intestinal Microbiota In Vitro
Authors: Zhou Qingqing , Xie Zuorui , Wu Danli , Liu Lingli , Shi Yongqing , Li Ping , Gu Qing
Published: October 2022

Microbiota-derived tryptophan metabolites are essential signals for maintaining gut homeostasis, yet the potential contribution to modulating gut microbiota has been rarely investigated. In this study, Lactiplantibacillus plantarum ZJ316 (CCTCC No. M 208077) with a high production (43.14 μg/mL) of indole-3-lactic acid (ILA) was screened. ILA with 99.00% purity was prepared by macroporous resin, Sephadex G–25 and reversed-phase high-performance liquid chromatography. Purified ILA can effectively inhibit foodborne pathogens such as Salmonella spp., Staphylococcus spp., Escherichia coli and Listeria monocytogenes. In an in vitro model of the human gut microbiota, a medium-dose ILA (172 mg/L) intervention increased the average relative abundance of phyla Firmicutes and Bacteroidota by 9.27% and 15.38%, respectively, while Proteobacteria decreased by 14.36% after 24 h fermentation. At the genus level, the relative abundance of Bifidobacterium and Faecalibacterium significantly increased to 5.36 ± 2.31% and 2.19 ± 0.77% (p < 0.01), respectively. Escherichia and Phascolarctobacterium decreased to 16.41 ± 4.81% (p < 0.05) and 2.84 ± 1.02% (p < 0.05), respectively. Intestinal short-chain fatty acids, especially butyric acid, were significantly increased (2.98 ± 0.72 µmol/mL, p < 0.05) and positively correlated with Oscillospira and Collinsella. Overall, ILA has the potential to regulate the gut microbiota, and an in-depth understanding of the relationship between tryptophan metabolites and gut microbiota is needed in the future.
Characteristics and Microbiome Profiling of Korean Gochang Bokbunja Vinegar by the Fermentation Process
Authors: Seo Hoonhee , Lee Saebim , Park Hyuna , Jo Sujin , Kim Sukyung , Rahim Md Abdur , Ul-Haq Asad , Barman Indrajeet , Lee Youngkyoung , Seo Ayoung , Kim Mijung , Jung Il-yun , Song Ho-Yeon
Published: October 2022

As NGS (next-generation sequencing) technology develops, metagenomics-based microbial ecology, that is, microbiome research, has recently led to the science of fermented food. Based on the above technology, a study was conducted to understand the characteristics of vinegar made from bokbunja, a local crop in Gochang-gun, Korea. Physicochemical characteristics of vinegar, organic acid analysis, microbial community analysis, and electronic tongue analysis were explored while fermenting the vinegar for 70 days under eight fermentation conditions according to the concentration of bokbunja liquid (100% or 50%), type of fermenter (porcelain jar or stainless container), and fermentation environment (natural outdoor conditions or temperature/oxygen controlled). As a result, distinct microbial community patterns were found in the stage of acetic acid fermentation and, accordingly, this fermentation of Gochang vinegar is classified into three categories. Vinegar prepared by the traditional method of outdoor fermentation using jars showed characteristics of “Acetobacter (42.1%)/Lactobacillus (56.9%) fusion fermentation”. Under conditions where oxygen and temperature were controlled indoors using jars, characteristics of “Komagataeibacter (90.2%) fermentation” were found. “Lactobacillus (92.2%) fermentation” characteristics were discovered under natural outdoor conditions using stainless steel containers. These fermentation pattern differences were related to taxonomic phylogenetic diversity, which was also considered involved in determining organic acid production and taste. These results will be helpful as a scientific basis for understanding the fermentation characteristics of Gochang vinegar and developing high-value-added traditional vinegar products.
In Vitro Bioaccessibility of Selenium from Commonly Consumed Fish in Thailand
Authors: Singhato Alongkote , Judprasong Kunchit , Sridonpai Piyanut , Laitip Nunnapus , Ornthai Nattikarn , Yafa Charun , Chimkerd Chanika
Published: October 2022

Selenium (Se), abundantly obtained in fish, is a crucial trace element for human health. Since there are no data on Se bioaccessibility from commonly consumed fish in Thailand, this study assessed the in vitro bioaccessibility of Se using the equilibrium dialyzability method. The five fish species most commonly consumed in Thailand were selected to determine total Se content using several preparation methods (fresh, boiling, and frying). Equilibrium dialyzability was used to perform in vitro bioaccessibility using enzymatic treatment to simulate gastrointestinal digestion for all boiled and fried fish as well as measuring Se using inductively coupled plasma triple quadrupole mass spectrometry (ICP-QQQ-MS). Two-way ANOVA with interaction followed by Tukey’s honestly significant difference (HSD) post hoc test revealed that boiled Indo-Pacific Spanish mackerel, longtail tuna, and short-bodied mackerel were significantly higher in Se content than striped snakehead and giant sea perch (p < 0.05). For fried fish, longtail tuna showed the highest Se content (262.4 µg/100 g of product) and was significantly different compared to the other fish (p < 0.05, estimated marginal means was 43.8–115.6 µg/100 g of product). Se bioaccessibilities from striped snakehead (70.0%) and Indo-Pacific Spanish mackerel (64.6%) were significantly higher than for longtail tuna (p < 0.05). No significant difference in bioaccessibility was found in terms of preparation method (i.e., boiling and frying). In conclusion, the fish included in this study, either boiled or fried, have high Se content and are good sources of Se due to high bioaccessibility.
Impact of Pulsed Electric Fields and pH on Enzyme Inactivation and Bioactivities of Peptic Hydrolysates Produced from Bovine and Porcine Hemoglobin
Authors: Sanchez-Reinoso Zain , Todeschini Sarah , Thibodeau Jacinthe , Ben Said Laila , Fliss Ismail , Bazinet Laurent , Mikhaylin Sergey
Published: October 2022

The production of bioactive peptides from hemoglobin via peptic hydrolysis is a promising alternative to valorizing slaughterhouse blood proteins. Nevertheless, it has some limitations such as low yield, high cost of enzymes, and the use of chemical reagents. The latter is aggravated by the pH increase to inactivate the enzyme, which can affect the bioactivity of the peptides. Thus, this study aimed to evaluate the effect of pulsed electric fields (PEF) on the pepsin inactivation and biological activities (antimicrobial and antioxidant) of hemoglobin hydrolysates. Bovine (Hb-B) and porcine (Hb-P) hemoglobin were hydrolyzed with pepsin for 3 h and treated with PEFs to inactivate the enzyme. The degree of hydrolysis (DH) did not show significant changes after PEF inactivation, whereas peptide population analysis showed some changes in PEF-treated hydrolysates over time, suggesting residual pepsin activity. PEF treatments showed no significant positive or negative impact on antimicrobial and antioxidant activities. Additionally, the impact of pH (3, 7, and 10) on bioactivity was studied. Higher pH fostered stronger anti-yeast activity and DPPH-scavenging capacity, whereas pH 7 fostered antifungal activity. Thus, the use of hemoglobin from the meat industry combined with PEF treatments could fit the circular economy concept since bioactive peptides can be produced more eco-efficiently and recycled to reduce the spoilage of meat products. Nevertheless, further studies on PEF conditions must be carried out to achieve complete inactivation of pepsin and the potential enhancement of peptides’ bioactivity.
Effects of Reheating Methods on Rheological and Textural Characteristics of Rice Starch with Different Gelatinization Degrees
Authors: Jiang Jiani , Li Jiangtao , Han Wenfang , Yang Qiqi , Liu Qiongxiang , Xiao Huaxi , Lin Qinlu , Fang Yong
Published: October 2022

Pregelatinized starch (PGS) is often used to improve the processing quality of foodstuffs, but little attention has been paid to the effects of different reheating methods and degree of starch gelatinization (DSG) on their rheological and textural properties. In this study, pregelatinized rice starches (RS) with gelatinization degrees ranging from 58% to 100% were prepared via different Rapid Visco Analyser (RVA) heating procedures and reheated in various methods, including high-power microwave (HM), low-power microwave (LM), and water bath. The rheological behavior and textural properties were explored, and the results demonstrated that the consistency, gel strength, hardness, and springiness of PGS in all tested samples decreased significantly after reheating. The storage modulus (G’) of PGS increased dramatically while the thermal stability decreased. Interestingly, the reheating methods possessed various effects on the starch of different DSG.
A Rapid Procedure for the Simultaneous Determination of Eugenol, Linalool and Fatty Acid Composition in Basil Leaves
Authors: Lenti Lucia , Rigano Daniela , Woo Sheridan L. , Nartea Ancuta , Pacetti Deborah , Maggi Filippo , Fiorini Dennis
Published: October 2022

Eugenol and linalool are often the most abundant volatile compounds found in basil (Ocimum basilicum L., Lamiaceae) leaves, and they are interesting for the aroma they provide and for their numerous beneficial bioactivities. Their determination is thus needed for several purposes. In the present study, to avoid the previous isolation of essential oil, the direct solvent extraction is proposed coupled with a transmethylation to convert acyl lipids into fatty acids methyl esters (FAMEs), thus assessing the possible simultaneous analysis of eugenol and linalool with FAMEs by gas chromatography coupled to flame ionization detector (GC-FID). The method has been validated and applied to ten basil leaves samples in which eugenol and linalool were found in mean concentrations of 2.80 ± 0.15 and 1.01 ± 0.04 g kg−1 (dry weight), respectively. FAMEs composition was dominated by linolenic acid (52.1–56.1%) followed by palmitic acid (19.3–22.4%) and linoleic acid (9.6–11.3%). The ratio of n6-polyunsaturated fatty acids (PUFAs)/n3-PUFAs was in the range of 0.17–0.20 in the investigated samples. The proposed method exploits a rapid procedure requiring 40 min, making use of a small amount of solvent and allowing the simultaneous determination of molecules contributing to assess the quality of this worldwide appreciated herb.
Copper-Modified Double-Emission Carbon Dots for Rapid Detection of Thiophanate Methyl in Food
Authors: Yue Xiaona , Zhu Chunna , Gu Rongrong , Hu Juan , Xu Yang , Ye Sheng , Zhu Jing
Published: October 2022

The detection of food safety and quality is very significant throughout the food supply. Stable dual-emission copper-modified fluorescent carbon dots (Cu-CDs) were successfully synthesized by a simple and environment-friendly hydrothermal, which was used for the real-time detection of pesticide residues in agricultural products. By optimizing the reaction conditions, Cu-CDs showed two emission peaks, with the highest fluorescence intensities at 375 and 450 nm. The structure, chemical composition and optical properties of Cu-CDs were investigated by XRD, TEM and IR. The results showed that thiophanate methyl (TM) could induce fluorescence quenching of Cu-CDs with no other ligands by the electron transfer through π-π stacking. The synchronous response of the dual-emission sensor enhanced the specificity of TM, which showed remarkable anti-interference capability. The fluorescence quenching degree of Cu-CDs had a good linear relationship with the TM concentration; the low detection limit for a pear was 0.75 μM, and for an apple, 0.78 μM. The recoveries in the fruit samples were 79.70–91.15% and 81.20–93.55%, respectively, and the relative standard deviations (RSDs) were less than 4.23% for the pear and less than 3.78% for the apple. Thus, our results indicate the feasibility and reliability of our methods in detecting pesticide residues in agricultural products.
Effects of Glucono-δ-Lactone and Transglutaminase on the Physicochemical and Textural Properties of Plant-Based Meat Patty
Authors: Kim Haesanna , Lee Mi-Yeon , Lee Jiseon , Jo Yeon-Ji , Choi Mi-Jung
Published: October 2022

Due to growing interest in health and sustainability, the demand for replacing animal-based ingredients with more sustainable alternatives has increased. Many studies have been conducted on plant-based meat, but only a few have investigated the effect of adding a suitable binder to plant-based meat to enhance meat texture. Thus, this study investigated the effects of the addition of transglutaminase (TG) and glucono-δ-lactone (GdL) on the physicochemical, textural, and sensory characteristics of plant-based ground meat products. The addition of a high quantity of GdL(G10T0) had an effect on the decrease in lightness (L* 58.98) and the increase in redness (a* 3.62). TG and GdL also decreased in terms of cooking loss (CL) and water holding capacity (WHC) of PBMPs. G5T5 showed the lowest CL (3.8%), while G3T7 showed the lowest WHC (86.02%). The mechanical properties also confirmed that G3T7-added patties have significantly high hardness (25.49 N), springiness (3.7 mm), gumminess (15.99 N), and chewiness (57.76 mJ). The improved textural properties can compensate for the chewability of PBMPs. Although the overall preference for improved hardness was not high compared to the control in the sensory test, these results provide a new direction for improving the textural properties of plant-based meat by using binders and forming fibrous structures.
Codon Pattern and Compositional Constraints Determination of Genes Associated with Chronic Periodontitis
Authors: Khandia Rekha , Pandey Megha , Rzhepakovsky Igor Vladimirovich , Khan Azmat Ali , Legaz Isabel
Published: October 2022

Genome-wide association studies showed the relationship of NIN, ABHD12B, WHAMM, AP3B2, and SIGLEC5 with chronic periodontitis. The study’s objective was to investigate different molecular patterns and evolutionary forces acting on the mentioned genes. The investigation of molecular patterns encompasses the study of compositional parameters, expression profile, physical properties of genes, codon preferences, degree of codon bias, determination of the most influential codons, and assessment of actions of evolutionary forces, such as mutations and natural selection. The overall compositional analysis revealed the dominance of A and G nucleotides compared to T and C. A relatively low codon usage bias is observed. The CTG codon is the most overused codon, followed by TCC. The genes, AP3B2 and SIGLEC5, preferred GC-ending codons, while NIN, ABHD12B, and WHAMM preferred AT-ending codons. The presence of directional mutational force and natural selection was found to operate codon usage in genes envisaged, and selective forces were dominant over mutational forces. Apart from mutation and selection forces, compositional constraints also played imperative roles. The study enriched our knowledge of specific molecular patterns associated with the set of genes significantly associated with chronic periodontitis. Further studies are warranted to identify more genetic signatures associated with the disease.
A Theory of Abrupt Climate Changes: Their Genesis and Anatomy
Authors: Ou Hsien-Wang
Published: October 2022

We combine our ice-sheet and climate models to formulate a deductive theory of abrupt climate changes pertaining to Heinrich/Dansgaard–Oeschger (H/DO) cycles and the last deglaciation punctuated by the Younger Dryas (YD). Since they are all accompanied by ice-rafted debris, we posit their common origin in the calving of the ice sheet due to a thermal switch at its bed, which naturally endows abruptness to these climate signals of the millennial timescale characteristics of the ice-mass balance. To distinguish the H/DO cycles, we differentiate the thermal triggers by geothermal-heat/surface-melt in the calving of inland/coastal ice, which provide their respective freshwater sources. Since surface-melt requires post-H warmth during the glacial, but is already operative in the Holocene, the DO cycles are encased within the H cycle during the glacial, but self-sustaining in the Holocene. They otherwise share the same time signature, thus resolving this seeming puzzle of commonality without invoking unknown climate forcing. The DO cycles transcend deglaciation to produce the observed sequence, but the calving-induced freshwater flux needs to be boosted by the rerouting of continental meltwater to cause YD. We discern a key process of an eddying ocean in its millennial adjustment toward maximum entropy production (MEP), which would melt the H-induced sea ice to cause an abrupt post-H warming followed by a gradual cooling that anchors the DO cycles to form the hierarchical Bond cycle. Since the modelled anatomies resemble the observed ones, our theory may provide a robust and unified account of abrupt climate changes.
Stress–Strain–Time Description and Analysis of Frozen–Thawed Silty Clay under Low Stress Level
Authors: Qu Haigang , Mu Dianrui , Ren Zhenlu , Huang Ziyuan , Huang Yang , Tang Aiping
Published: October 2022

The construction of high-speed railways in cold regions needs to consider the effects of freeze–thaw cycles (FTHs) on the long-term deformation of subgrades. However, at present, research on the creep characteristics of frozen–thawed rocks and soils is not extensive. In the limited studies on frozen–thawed soil creep properties, current research focuses more on high stress–strain–time responses, but for the subgrades, the inner stress is usually low. This paper presents the results of triaxial compression creep tests on remolded, frozen–thawed silty clay sampled in the Yichun-Tieli area and describes its stress–strain–time relationship in an arctan function-based mathematical model. Each creep test condition is studied using three specimens. Frozen–thawed silty clay exhibits attenuation creep under low-level stress. In general, from 4 FTHs to 11 FTHs, the mean elasticity modulus decreases first, and then, increases. The exerted stress is higher than the yield stress; the more FTHs the specimens experience, the more time they need to be deformed stably under the same axial deviatoric stress (ADS). Under the same mean ADS, the mean stable strain of 7 FTHs exceeds the other two FTH conditions and, in general, the mean stable strain of 4 FTHs exceeds 11 FTHs. By dissecting the phenomena, it can be concluded that with FTHs increasing, moisture and voids reconstitute in the process; the elastic strain accounts for most of the total strain and significantly decides the extent of creep deformation; the arctan function-based model is basically able to describe, but not perfectly predict, the stress–strain–time relationship of frozen–thawed silty clay.
Blood Pressure Measurement: From Cuff-Based to Contactless Monitoring
Authors: Man Ping-Kwan , Cheung Kit-Leong , Sangsiri Nawapon , Shek Wilfred , Wong Kwan-Long , Chin Jing-Wei , Chan Tsz-Tai , So Richard
Published: October 2022

Blood pressure (BP) determines whether a person has hypertension and offers implications as to whether he or she could be affected by cardiovascular disease. Cuff-based sphygmomanometers have traditionally provided both accuracy and reliability, but they require bulky equipment and relevant skills to obtain precise measurements. BP measurement from photoplethysmography (PPG) signals has become a promising alternative for convenient and unobtrusive BP monitoring. Moreover, the recent developments in remote photoplethysmography (rPPG) algorithms have enabled new innovations for contactless BP measurement. This paper illustrates the evolution of BP measurement techniques from the biophysical theory, through the development of contact-based BP measurement from PPG signals, and to the modern innovations of contactless BP measurement from rPPG signals. We consolidate knowledge from a diverse background of academic research to highlight the importance of multi-feature analysis for improving measurement accuracy. We conclude with the ongoing challenges, opportunities, and possible future directions in this emerging field of research.
Consideration of Maillard Reaction-Based Time–Temperature Indicator (TTI) to Visualize Shelf Life of Cold-Stored Strawberries
Authors: Cho Byeong-Hyo , Lee Jung-Hyun
Published: October 2022

In this paper, the potential of a Maillard reaction-based time–temperature indicator (TTI) as a device for the visualization of shelf life was evaluated by comparing the quality variations of cold-stored strawberries and the color changes of Maillard reaction solutions. The color variations of the Maillard reaction solutions stored in the same storage environment as the cold-stored strawberries showed suitable characteristics for the visualization of shelf life, such as pronounced color changes, a wide-ranging color variation rate, and activation energy. In particular, the concentrations of the reaction solutions with the combinations of 3.0 M D-xylose, 1.5 M glycine, and 0.3 M Na2HPO4; 2.0 M D-xylose, 1.5 M glycine, and 0.3 M Na2HPO4; and 4.0 M D-xylose, 1.0 M glycine, and 0.3 M Na2HPO4 were suitable for the visualization of the weight loss variation, color difference variation, and appearance damage. These concentrations showed similar color variations and Arrhenius activation energies to the variation of the quality characteristics, such as weight loss and color difference. Additionally, a Maillard reaction-based time–temperature indicator (TTI) has a wide applicability to other agricultural products using the wide range of the color change rate and the activation energy.
Assessing the Reliability of Treated Grey Water Irrigation on Soil and Tomatoes (Solanum lycopersicum L.)
Authors: Hajlaoui Hichem , Akrimi Rawaa , Guesmi Anis , Hachicha Mohamed
Published: October 2022

Under a water scarcity situation, it is expected to manage water more efficiently. This study aims to evaluate the effect of treated grey water (from laundry and tableware), pre-treated through a wetland mini-reactor with a horizontal underground flow, on soil and tomatoes. The experiment included two tomato cultivars (Dart and Firenze), planted in completely randomized bloc design, and irrigated with treated grey water (TGW) vs. ground water (C) as control. Soil, tomato leaves and fruits were assessed for microbial contamination. Tomato yield, physico-chemical characteristics and antioxidant contents were studied. Results showed that TGW met the standards for irrigation water for most water quality variables. Irrigation with TGW increased the concentrations of phosphorus (P), iron (Fe) and copper (Cu) in the soil. Although, the soil ionic composition was still in the suitable range for agriculture. Opportunely, there was no contamination by fecal coliforms, streptococcus and E. coli in soil and tomatoes. Dart cultivar seems to be more responsive to TGW and had higher fruit number and weight. This response was accompanied by an effective antioxidant response, higher water and juice content. The findings of this study emphasize that TGW may provide a way to preserve water resources and to avoid soil contamination.
Activities of H2O2-Converting Enzymes in Apple Leaf Buds during Dormancy Release in Consideration of the Ratio Change between Bud Scales and Physiologically Active Tissues
Authors: Hubmann Anna M. , Jammer Alexandra , Monschein Stephan
Published: October 2022

Hydrogen peroxide-converting enzyme activities in leaf buds of the apple cultivar Idared during the transition from dormancy release to the ontogenetic development were investigated. For this purpose, leaf buds were collected from 26 March 2021 (DOY = day of the year 85) to 23 April 2021 (DOY 113) and the air temperature was continuously monitored. Enzyme assay protocols for catalase (CAT), intracellular peroxidase (POX), and cell wall-bound peroxidase (cwPOX) in apple leaf buds were successfully established based on published protocols. All enzymes showed considerable changes in activity during the observation period. Fluctuation in daily mean air temperatures seemed not to affect the activities of POX and CAT, whereas severe drops in daily mean air temperature may have interrupted the assumed trajectory of cwPOX activity during the stage of ontogenetic development. In addition, the importance of considering changes in the ratio between physiologically active tissues and bud scales when investigating physiological changes in buds during the phase of dormancy release and ontogenetic development is discussed. A new reference system, namely the “adjusted dry weight” [aDW], is proposed to circumvent this shift in ratios when working with scaled buds.
Phenological Study of 53 Spanish Minority Grape Varieties to Search for Adaptation of Vitiviniculture to Climate Change Conditions
Authors: Muñoz-Organero Gregorio , Espinosa Francisco E. , Cabello Félix , Zamorano José Pablo , Urbanos Miguel A. , Puertas Belén , Lara Miguel , Domingo Carme , Puig-Pujol Anna , Valdés M. Esperanza , Moreno Daniel , Diaz-Losada Emilia , Martínez M. Carmen , Santiago José L. , Cibriain José F. , Raboso Eva , Fernández-Pastor Marta
Published: October 2022

The main phenological stages (budburst, flowering, veraison, and ripeness) of 53 Spanish minority varieties were studied to determine their potential to help winegrowers adapt to climate change conditions. In total, 43 varieties were studied in the same location in Spain (Alcalá de Henares, in the Madrid region) and 10 varieties in 5 other regions (Galicia, Navarre, Catalonia, Extremadura, and Andalusia). Other traits of agronomic and oenological interest, such as yield and acidity, were also monitored. The results allow for the grouping of the varieties into several clusters according to the time of ripeness (very early—only for red varieties—and early, intermediate, and late, for both red and white varieties) and yield (high, medium, and low). The total acidity in the grape juice ranged from 3 to 11 g of tartaric acid/L. The average temperatures were higher (up to 3–4 °C during summer) compared to historical averages during the 1957–2021 time period. Advanced phenology phases and reduced acidity are regarded as negative effects of climate change for winegrowing practices. Since some minority varieties showed late or intermediate ripening, high acidity, and high (1 Kg/shoot) or medium (0.5 Kg/shoot) yield, our findings suggest that they may be cultivated in the coming years by winegrowers as an approach to mitigate climate change effects.
Implementation of the Patient Counselling Service at the Cancer Hospital in Radom, Poland
Authors: Grzyb Katarzyna , Meresińska Martyna , Religioni Urszula , Juszczyk Grzegorz , Płaczek Jakub , Neumann-Podczaska Agnieszka , Szymański Filip M. , Chełstowska Beata , Wieczorowska-Tobis Katarzyna , Cofta Szczepan , Tobis Sławomir , Staszewski Rafał , Vaillancourt Regis , Majewski Rafał , Hernik Justyna , Fehir Sola Katarina , Blicharska Eliza , Kaźmierczak Justyna , Rutkowska Ewa , Prygiel Elżbieta , Skierska Monika , Nawara Monika , Korbiewska Izabela , Krysiński Jerzy , Merks Piotr
Published: October 2022

Background: Non-adherence occurs in various groups of patients, including those with chronic diseases. One strategy to increase adherence among oncological patients is to individualise treatment and expand pharmaceutical care. Pharmaceutical labels that remind patients how they should take their medications are of great importance in this respect. Objective: The main objective of this study was to evaluate medication adherence in oncological patients, and to gather their opinions on the individual medication labelling system as an element of effective treatment. Methods: The study was conducted in 2021 among 82 patients of the oncological department of the Centre of Oncology in Radom. The research tool was a questionnaire consisting of personal data and two parts relating to the patient’s disease and the medication labelling system. Results: Nearly half of the respondents reported that they forget to take medications and how they should take them. These problems increased with the age of the patient and the number of administered medications. Of the respondents, 89% stated that the labels with dosing information are helpful. Over 67% agreed that these labels should be affixed to all medications. Nearly 90% of the respondents believed the labels should be available in all pharmacies. Conclusions: Non-adherence is a common phenomenon among oncological patients. Pharmacists providing a labelling service for medicinal products can play a significant role in reducing this phenomenon.
Testifying after an Investigation: Shaping the Mental Health of Public Safety Personnel
Authors: Ricciardelli Rosemary , Carleton R. Nicholas , Anschuetz Barbara , Gravel Sylvio , McKay Brad
Published: October 2022

In this editorial, we draw on two Canadian cases to interrogate how mass causality events and investigations consume many responders before (e.g., public safety communicators, detachment service assistants), during (e.g., police, fire, paramedics), and after the incident (e.g., coroners, correctional workers, media coverage). Their well-being may suffer from the associated processes and outcomes. In the current article, we focus on the mass causality incident of 2020 in Nova Scotia, Canada, and the investigation following a prisoner death in 2019 in Newfoundland, Canada, to explore how testifying post-incident can be made more palatable for participating public safety personnel (PSP). Specifically, we study how testifying after an adverse event can affect PSP (e.g., recalling, vicarious trauma, triggers) and how best to mitigate the impact of testimony on PSP well-being, with a lens to psychological “recovery” or wellness. We focus here on how to support those who may have to testify in a judicial proceeding or official inquiry, given being investigated for best-intended actions can result in moral injury or a posttraumatic stress injury, both exacerbated by judicial review, charge, accusation, or inquiry.
Chronotype and Social Jetlag Influence Performance and Injury during Reserve Officers’ Training Corps Physical Training
Authors: McGinnis Graham R. , Thompson Shani T. , Aguilar Charli D. , Dial Michael B. , Tandy Richard D. , Radzak Kara N.
Published: October 2022

Sleep and circadian rhythms are critically important for optimal physical performance and maintaining health during training. Chronotype and altered sleep may modulate the response to exercise training, especially when performed at specific times/days, which may contribute to musculoskeletal injury. The purpose of this study was to determine if cadet characteristics (chronotype, sleep duration, and social jetlag) were associated with injury incidence and inflammation during physical training. Reserve Officers’ Training Corps (ROTC) cadets (n = 42) completed the Morningness/Eveningness Questionnaire to determine chronotype, and 1-week sleep logs to determine sleep duration and social jetlag. Salivary IL-6 was measured before and after the first and fourth exercise sessions during training. Prospective injury incidence was monitored over 14 weeks of training, and Army Physical Fitness Test scores were recorded at the conclusion. Chronotype, sleep duration, and social jetlag were assessed as independent factors impacting IL-6, injury incidence, and APFT scores using ANOVAs, chi-squared tests, and the t-test where appropriate, with significance accepted at p < 0.05. Evening chronotypes performed worse on the APFT (evening = 103.8 ± 59.8 vs. intermediate = 221.9 ± 40.3 vs. morning = 216.6 ± 43.6; p < 0.05), with no difference in injury incidence. Sleep duration did not significantly impact APFT score or injury incidence. Social jetlag was significantly higher in injured vs. uninjured cadets (2:40 ± 1:03 vs. 1:32 ± 55, p < 0.05). Exercise increased salivary IL-6, with no significant effects of chronotype, sleep duration, or social jetlag. Evening chronotypes and cadets with social jetlag display hampered performance during morning APFT. Social jetlag may be a behavioral biomarker for musculoskeletal injury risk, which requires further investigation.
Experimental Comparison of Biofidel Measuring Devices Used for the Validation of Collaborative Robotics Applications
Authors: Zimmermann Jan , Huelke Michael , Clermont Matthias
Published: October 2022

Biofidel measuring devices are used to validate safety in collaborative workplaces. In these workplaces, humans work together with robots that are equipped with a Power and Force Limiting function (PFL). In this experimental comparison, differences between devices and possible causes are examined more closely. Safety-related parameters are identified in a literature review. Focusing on mechanical aspects, three biofidel measuring devices are analysed and compared in an experimental test series. To this end, a linear motor and a pendulum are used and the steps for comparing concepts are proposed and applied. Depending on the stiffness settings and the materials used, geometry effects on the force-deformation behaviour are shown. An oscillation occurred in one case. The comparison of the three devices shows average differences of 5% in measured peak force between them. This study helps to achieve uniform and comparable results in practice.
Emotion Regulation and Psychological Capital of Chinese University Students during the COVID-19 Pandemic: The Serial Mediation Effect of Learning Satisfaction and Learning Engagement
Authors: Tang Yuxi , He Weiguang
Published: October 2022

The mediating mechanism between the emotion regulation and psychological capital of university students is currently unclear. This study analyzed the serial mediation of learning satisfaction and learning engagement on the relationship between the emotion regulation and psychological capital of university students during the coronavirus disease 2019 (COVID-19) pandemic. A total of 328 undergraduates and postgraduates from universities in different regions of China were surveyed through an online questionnaire. The tools used in the study were the emotion regulation questionnaire, university student learning satisfaction questionnaire, learning engagement questionnaire, and psychological capital questionnaire. The analysis revealed both direct and indirect mediation effects. It was found that emotion regulation can positively predict psychological capital. Further, learning satisfaction and learning engagement can act as mediating variables between emotion regulation and psychological capital, respectively. Learning satisfaction and learning engagement can also have a serial mediation effect between emotion regulation and psychological capital. The results show that learning support should be strengthened to improve the learning satisfaction and learning engagement of students and, consequently, enhance their psychological capital.
Study on Urban Resilience from the Perspective of the Complex Adaptive System Theory: A Case Study of the Lanzhou-Xining Urban Agglomeration
Authors: Yang Liangjie , Yang Hainan , Zhao Xueyan , Yang Yongchun
Published: October 2022

In the context of global environmental change and continuous urbanization, enhancing urban resilience is an important way to improve urban emergency management capacity and achieve sustainable development of urban systems. It is of great significance to clarify the mechanisms and effects of urban resilience and carry out resilience measurement to improve the level of urban system resilience and alleviate the pressure of environmental disturbances on the stable operation of urban systems. As an important part of the “Belt and Road” Initiative and one of the few leading economic regions in western China, promoting the high-quality development of the Lanzhou-Xining urban agglomeration is of profound significance for strengthening ethnic unity and stabilizing the northwest and southwest regions. Based on the complex adaptive system (CAS) theory and the adaptive cycle model, this study understands urban resilience as the comprehensive result of urban system stability, self-organization, learning adaptability and transformability, constructs a multi-level open index evaluation system, and analyzes the spatio-temporal evolution characteristics of urban resilience of the Lanzhou-Xining urban agglomeration from the proposed design to the formal planning in 2010–2017. The findings are as follows: (1) Research on the urban resilience of the Lanzhou-Xining urban agglomeration verifies the applicability of the evolutionary urban resilience analysis framework and makes preliminary findings on urban resilience based on CAS theory, which provide a certain theoretical reference for the research on the spatio-temporal evolution of urban resilience. (2) From 2010 to 2017, significant differences are observed between various urban attributes. Resilience exhibits an overall upward trend, and spatial evolution changes from a double core (Lanzhou and Xining) to three cores (Lanzhou, Xining and Haidong) and polycentric modes. (3) Based on urban resilience characteristics and an urban system adaptability cycle model, this paper divides the Lanzhou-Xining urban agglomeration cities into four types (exploitation–reorganization, conservation–release, conservation–exploitation and exploitation), and proposes corresponding adaptive management countermeasures. These could be adopted as a reference to promote the high-quality development of the Lanzhou-Xining urban agglomeration.
Impact of the New Coronavirus Infection on the Immune System of Children and Adolescents in the Region of the Russian Federation
Authors: Kostarev Sergey , Komyagina Oksana , Fayzrakhmanov Rustam , Kurushin Daniel , Tatarnikova Natalya , Novikova (Kochetova) Oksana , Sereda Tatyana
Published: October 2022

The emergence of COVID-19 (SARS-CoV-2) has presented public health professionals with new challenges in the diagnosis of the disease and treatment of patients. Nowadays, the epidemiology, clinical features, prevention and treatment of the disease are studied poorly due to continuous mutation of the pathogen. One of the consequences of the new coronavirus infection could be changes in the immune system of the human population. A detailed analysis of the immunological status of different racial groups under the influence of the new coronavirus infection is currently studied insufficiently, making this work of particular relevance. There is also a reluctance among some Russian residents to be vaccinated, including the population of Perm Krai, due to a lack of research on possible deviations in cellular immunity due to SARS-CoV-2 vaccination. At the start of the third wave caused by the new coronavirus infection, only 40% of the Russian population had been vaccinated, which was insufficient to acquire collective immunity. In the autumn of 2021, a QR code measure was introduced for vaccinated residents, which resulted in exceeding the necessary barrier for acquiring collective immunity. Due to the high growth and severity of the disease, we analysed the immunograms of children and adolescents, aged from 5 months to 17 years, in Perm Krai during the pandemic years 2020–2021. The patients’ immunological status results were divided into three categories. Laboratory diagnosis of the human immune system was carried out using serological and flow cytophotometric analyses. A total of 247 samples were analysed. The aim of this work was to investigate changes in the immune system of children and adolescents during the pandemic caused by the new coronavirus infection. The methodology was based on the analysis of immunograms, including biochemical studies, immune status and flow cytophotometric analysis. The immunograms were pre-sorted by IgA, IgM, IgG immunoglobulin status into four categories: absence of disease—k1 in which IgA, IgM, IgG immunoglobulin values were within the reference interval, active disease stage—k2 in which IgA, IgM immunoglobulins had gone beyond the reference interval, passive disease stage—k3 characterised by IgG and IgM immunoglobulin status, and patient recovery process—k4. In the immunograms, three immune status indicators were selected for further investigation: phagocytosis absolute value, phagocytic number and phagocytic index and five flow cytometry indices: leukocytes, lymphocytes, NK cells (CD16+CD56+), T helpers (CD3+CD4+) and CD4+/CD8+ immunoregulation index. A quantitative analysis of the deviations of these indicators from the reference intervals was performed in the three studied age groups of children and adolescents living in Perm Krai of the Russian Federation during the pandemic of 2020–2021.
Motor Vehicle Crash and Hospital Charges in Front- and Rear-Seated Restrained and Unrestrained Adult Motor Vehicle Occupants
Authors: Pressley Joyce C. , Pawlowski Emilia , Hines Leah M. , Bhatta Sabana , Bauer Michael J.
Published: October 2022

There are reports that historically higher mortality observed for front- compared to rear-seated adult motor vehicle (MV) occupants has narrowed. Vast improvements have been made in strengthening laws and restraint use in front-, but not rear-seated occupants suggesting there may be value in expanding the science on rear-seat safety. Methods. A linked 2016–2017 hospital and MV crash data set, the Crash Outcomes Data Evaluation System (CODES), was used to compare characteristics of front-seated (n = 115,939) and rear-seated (n = 5729) adults aged 18 years and older involved in a MV crash in New York State (NYS). A primary enforced seat belt law existed for front-seated, but not rear-seated occupants. Statistical analysis employed SAS 9.4. Results. Compared to front-seated occupants, those rear-seated were more likely to be unrestrained (21.2% vs. 4.3%, p < 0.0001) and to have more moderate-to-severe injury/death (11.9% vs. 11.3%, p < 0.0001). Compared to restrained rear-seated occupants, unrestrained rear-seated occupants had higher moderate-to-severe injury/death (21.5% vs. 7.5%, p < 0.0001) and 4-fold higher hospitalization. More than 95% of ejections were unrestrained and had 7-fold higher medical charges. Unrestrained occupants’ hospital stays were longer, charges and societal financial costs higher. Conclusions. These findings extend the science of rear-seat safety in seriously injured rear-seated occupants, document increased medical charges and support the need to educate consumers and policy makers on the health and financial risks of adults riding unrestrained in the rear seat. The lack of restraint use in adult rear-seated motor vehicle occupants consumes scarce health care dollars for treatment of this serious, but largely preventable injury.
Retrospective Self-Reports of How Adolescent Substance Use Changed with the COVID-19 Pandemic
Authors: Leung Janni , Quinn Catherine , Carlyle Molly , Ellem Rhiannon , Tisdale Calvert , Davidson Lily , White Melanie J. , Kavanagh David J. , Hides Leanne
Published: October 2022

The final year of high school is a challenging phase of adolescents’ lives and substance use can play an important role. We examined changes in the frequency and quantity of alcohol and cannabis use, and demographic correlates among Grade 12 students of 2020. Students (N = 844) from nine schools retrospectively self-reported changes in substance use after the easing of COVID-19 lockdowns (back to school), compared to before the pandemic. Changes in use were examined with age, gender, Aboriginal or Torres Islander, parental and family characteristics, and truancy. Thirty-one percent of students reported that they used alcohol less frequently, and 24% reported that they used it more frequently compared to pre-COVID-19. Most students (46%) reported that they used cannabis less, while a subset reported using more frequently (22%). A history of truancy was associated with an increased frequency (OR = 2.13 [1.18–3.83]) of cannabis use. A substantial minority of adolescents used more alcohol and cannabis after the initial COVID-19 lockdown period. Students in their final year who reported increased use may benefit from increased support to manage their substance use.
Research on the Efficiency of Green Agricultural Science and Technology Innovation Resource Allocation Based on a Three-Stage DEA Model—A Case Study of Anhui Province, China
Authors: Yao Sheng , Wu Guosong
Published: October 2022

In order to achieve sustainable development of agriculture, people have gradually begun to attach importance to the development of low-carbon agriculture and to regard green agricultural technology innovation and promotion as increasingly more important. Taking the Anhui Province of China as an example, this study analyzed the impact of green agricultural science and technology innovation resource allocation on rural revitalization by constructing an econometric model. We found that the overall efficiency of the overall allocation of agricultural science and technology innovation resources in Anhui Province increased in the sample period, but the scale efficiency level was relatively low. The key path to improving the overall efficiency of allocation was to improve the scale efficiency level. The allocation of agricultural science and technology innovation resources in 16 cities and prefectures performed well in terms of pure technical efficiency, but there were significant differences in scale efficiency, which further affected the overall allocation efficiency of different regions. Among them, the allocation efficiencies of agricultural science and technology innovation resources in Hefei and Fuyang were at the leading level in Anhui Province. Similar to the overall situation of the province, the improvement path of areas with low comprehensive efficiency lay in the improvement of scale efficiency. In view of this, from the policy level, we need to optimize the relationship between the government and the market, speed up the construction of platforms and carriers, attach importance to the construction of the agricultural science and technology talent training system, and improve the open sharing mechanism.