bluuebunny/crossref_metadata_2025 huggingface dataset:
The contribution of granger causality analysis to our understanding of cardiovascular homeostasis: from cardiovascular and respiratory interactions to central autonomic network control
Authors: Pichot Vincent , Corbier Christophe , Chouchou Florian
Published: August 2024

Homeostatic regulation plays a fundamental role in maintenance of multicellular life. At different scales and in different biological systems, this principle allows a better understanding of biological organization. Consequently, a growing interest in studying cause-effect relations between physiological systems has emerged, such as in the fields of cardiovascular and cardiorespiratory regulations. For this, mathematical approaches such as Granger causality (GC) were applied to the field of cardiovascular physiology in the last 20 years, overcoming the limitations of previous approaches and offering new perspectives in understanding cardiac, vascular and respiratory homeostatic interactions. In clinical practice, continuous recording of clinical data of hospitalized patients or by telemetry has opened new applicability for these approaches with potential early diagnostic and prognostic information. In this review, we describe a theoretical background of approaches based on linear GC in time and frequency domains applied to detect couplings between time series of RR intervals, blood pressure and respiration. Interestingly, these tools help in understanding the contribution of homeostatic negative feedback and the anticipatory feedforward mechanisms in homeostatic cardiovascular and cardiorespiratory controls. We also describe experimental and clinical results based on these mathematical tools, consolidating previous experimental and clinical evidence on the coupling in cardiovascular and cardiorespiratory studies. Finally, we propose perspectives allowing to complete the understanding of these interactions between cardiovascular and cardiorespiratory systems, as well as the interplay between brain and cardiac, and vascular and respiratory systems, offering a high integrative view of cardiovascular and cardiorespiratory homeostatic regulation.
NORSE/FIRES: how can we advance our understanding of this devastating condition?
Authors: Champsas Dimitrios , Zhang Xushuo , Rosch Richard , Ioannidou Evangelia , Gilmour Kimberly , Cooray Gerald , Woodhall Gavin , Pujar Suresh , Kaliakatsos Marios , Wright Sukhvir K.
Published: August 2024

Introduction

New onset refractory status epilepticus (NORSE) is a rare and devastating condition characterised by the sudden onset of refractory status epilepticus (RSE) without an identifiable acute or active structural, toxic, or metabolic cause in an individual without a pre-existing diagnosis of epilepsy. Febrile infection-related epilepsy syndrome (FIRES) is considered a subcategory of NORSE and presents following a febrile illness prior to seizure onset. NORSE/FIRES is associated with high morbidity and mortality in children and adults.

Methods and results

In this review we first briefly summarise the reported clinical, paraclinical, treatment and outcome data in the literature. We then report on existing knowledge of the underlying pathophysiology in relation toin vitroandin vivopre-clinical seizure and epilepsy models of potential relevance to NORSE/FIRES.

Discussion

We highlight how pre-clinical models can enhance our understanding of FIRES/NORSE and propose future directions for research.
Auditory guidance of eye movements toward threat-related images in the absence of visual awareness
Authors: Hu Junchao , Badde Stephanie , Vetter Petra
Published: August 2024

The human brain is sensitive to threat-related information even when we are not aware of this information. For example, fearful faces attract gaze in the absence of visual awareness. Moreover, information in different sensory modalities interacts in the absence of awareness, for example, the detection of suppressed visual stimuli is facilitated by simultaneously presented congruent sounds or tactile stimuli. Here, we combined these two lines of research and investigated whether threat-related sounds could facilitate visual processing of threat-related images suppressed from awareness such that they attract eye gaze. We suppressed threat-related images of cars and neutral images of human hands from visual awareness using continuous flash suppression and tracked observers’ eye movements while presenting congruent or incongruent sounds (finger snapping and car engine sounds). Indeed, threat-related car sounds guided the eyes toward suppressed car images, participants looked longer at the hidden car images than at any other part of the display. In contrast, neither congruent nor incongruent sounds had a significant effect on eye responses to suppressed finger images. Overall, our results suggest that only in a danger-related context semantically congruent sounds modulate eye movements to images suppressed from awareness, highlighting the prioritisation of eye responses to threat-related stimuli in the absence of visual awareness.
Effect of estradiol with or without micronized progesterone on cholinergic-related cognitive performance in postmenopausal women
Authors: Conley Alexander C. , Vega Jennifer N. , Johnson Julia V. , Dumas Julie A. , Newhouse Paul A.
Published: August 2024

Introduction

Women are at a higher risk of developing Alzheimer’s disease (AD), and the decline in estrogens post-menopause is thought of as a factor increasing this risk. Estradiol (E2) is important in supporting cholinergic neuronal integrity, and cholinergic functioning may be negatively impacted following the loss of E2 post-menopause. The use of exogenous E2 has been observed to enhance cholinergically mediated cognitive performance in healthy post-menopausal women, which indicates a potentially protective mechanism. However, E2 is often co-administered with progestin or progesterone to prevent endometrial proliferation. Progesterone/progestins have previously been shown to have a detrimental effect on E2-mediated biological and cognitive effects mediated by cholinergic systems in preclinical models, therefore the present study aimed to assess whether progesterone would modify the effect of E2 to influence cognition during cholinergic blockade.

Methods

Twenty participants completed 3-months of oral E2 treatment with micronized progesterone (mPRO) or with placebo (PLC) in a repeated-measures within-subjects crossover design, in which they also completed five anticholinergic challenge days per hormone treatment condition. During the challenge participants were administered low or high doses of the nicotinic cholinergic antagonist mecamylamine, the muscarinic cholinergic antagonist scopolamine, or placebo. Following drug administration participants performed cognitive tests sensitive to cholinergic tone, assessing attention, episodic memory, and working memory.

Results

Significant decrements were found on some tasks when participants were taking E2+mPRO compared to E2 alone. Specifically, under more challenging task conditions and larger anticholinergic doses, participants showed poorer performance on the Critical Flicker Fusion task and the Stroop test and responded more conservatively on the N-back working memory task. Other tasks showed no differences between treatments under cholinergic blockade.

Discussion

The findings show that mPRO when taken in concert with E2, was detrimental to effortful cognitive performance, in the presence of cholinergic blockade. These results are important for assessing the impact of combined postmenopausal hormone treatment on cognitive performance that is dependent on cholinergic functioning after menopause.
Dysregulation of the dopaminergic system secondary to traumatic brain injury: implications for mood and anxiety disorders
Authors: Mata-Bermudez Alfonso , Trejo-Chávez Ricardo , Martínez-Vargas Marina , Pérez-Arredondo Adán , Martínez-Cardenas Maria de Los Ángeles , Diaz-Ruiz Araceli , Rios Camilo , Navarro Luz
Published: August 2024

Traumatic brain injury (TBI) represents a public health issue with a high mortality rate and severe neurological and psychiatric consequences. Mood and anxiety disorders are some of the most frequently reported. Primary and secondary damage can cause a loss of neurons and glial cells, leading to dysfunction of neuronal circuits, which can induce imbalances in many neurotransmitter systems. Monoaminergic systems, especially the dopaminergic system, are some of the most involved in the pathogenesis of neuropsychiatric and cognitive symptoms after TBI. In this work, we summarize the studies carried out in patients who have suffered TBI and describe alterations in the dopaminergic system, highlighting (1) dysfunction of the dopaminergic neuronal circuits caused by TBI, where modifications are shown in the dopamine transporter (DAT) and alterations in the expression of dopamine receptor 2 (D2R) in brain areas with dopaminergic innervation, thus establishing a hypodopaminergic state and (2) variations in the concentration of dopamine and its metabolites in biological fluids of post-TBI patients, such as elevated dopamine (DA) and alterations in homovanillic acid (HVA). On the other hand, we show a large number of reports of alterations in the dopaminergic system after a TBI in animal models, in which modifications in the levels of DA, DAT, and HVA have been reported, as well as alterations in the expression of tyrosine hydroxylase (TH). We also describe the biological pathways, neuronal circuits, and molecular mechanisms potentially involved in mood and anxiety disorders that occur after TBI and are associated with alterations of the dopaminergic system in clinical studies and animal models. We describe the changes that occur in the clinical picture of post-TBI patients, such as alterations in mood and anxiety associated with DAT activity in the striatum, the relationship between post-TBI major depressive disorders (MDD) with lower availability of the DA receptors D2R and D3R in the caudate and thalamus, as well as a decrease in the volume of the substantia nigra (SN) associated with anxiety symptoms. With these findings, we discuss the possible relationship between the disorders caused by alterations in the dopaminergic system in patients with TBI.
Negative association between Body Roundness Index and bone mineral density: insights from NHANES
Authors: Ding Ziyao , Zhuang Zhe , Tang Rongze , Qu Xinzhe , Huang Zicheng , Sun Maji , Yuan Feng
Published: August 2024

Background

Osteoporosis (OP), affecting millions around the globe, is a prevalent degenerative condition of the bones characterized by a decrease in bone mineral density (BMD) and an increase in bone fragility. A novel anthropometric measure, the Body Roundness Index (BRI), provides a more accurate assessment of body fat distribution compared to traditional metrics. Using data from the National Health and Nutrition Examination Survey (NHANES), this study aims to explore the relationship between BRI and total BMD in U.S. adults aged 20 and above.

Methods

Data from NHANES (2011–2018) were examined, encompassing 9,295 participants following exclusions. Dual-energy X-ray absorptiometry (DXA) was employed to measure BMD. BRI was calculated using waist circumference (WC) and height. The study accounted for variables such as demographic traits, physical exam results, lab test findings, and survey responses. Weighted multivariable linear regression models and smooth curve fitting methods were utilized to assess the relationship between BRI and total BMD.

Results

The research found a notable inverse relationship between BRI and total BMD. In the model with full adjustments, an increase of one unit in BRI was linked to a 0.0313 g/cm2 reduction in total BMD (P < 0.0001). Moreover, an inflection point was identified at BRI = 9.5229, where each one-unit rise in BRI beyond this threshold corresponded to a more substantial decrease in total BMD (0.0363 g/cm2). Analysis by subgroups revealed that this negative association was consistent across most demographic and health-related categories.

Conclusions

The results demonstrate a notable inverse relationship between BRI and total BMD, indicating that a higher BRI could be associated with lower BMD and a potentially greater risk of developing OP. This underscores the significance of accounting for body fat distribution in preventing OP and advocates for the use of BRI as a valuable marker for early intervention approaches.
Imaging features and clinical evaluation of pulmonary nodules in children
Authors: Dilimulati Muheremu , Yuan Shuhua , Jiang Hejun , Wang Yahua , Ma Hui , Shen Shiyu , Lin Jilei , Chen Jiande , Yin Yong
Published: August 2024

Background

With the widespread use of computed tomography (CT), the detection rate of pulmonary nodules in children has gradually increased. Due to the lack of epidemiological evidence and clinical guideline on pulmonary nodule treatment in children, we aimed to provide a reference for the clinical diagnosis and management of pediatirc pulmonary nodules.

Methods

This retrospective study collected consecutive cases from April 2012 to July 2021 in the Shanghai Children’s Medical Center. The sample included children with pulmonary nodules on chest CT scans and met the inclusion criteria. All patients were categorized into tumor and non-tumor groups by pre-CT clinical diagnosis. Nodule characteristics between groups were analyzed. To establish a clinical assessment model for the benign versus malignant pulmonary nodules, patients who have been followed-up for three months were detected and a decision tree model for nodule malignancy prediction was constructed and validated.

Results

The sample comprised 1341 patients with an average age of 7.2 ± 4.6 years. More than half of them (51.7%) were diagnosed with malignancies before CT scan. 48.3% were diagnosed with non-tumor diseases or healthy. Compared to non-tumor group, children with tumor were more likely to have multiple nodules in both lungs, with larger size and often be accompanied by osteolytic or mass lesions. Based on the decision tree model, patients’ history of malignancies, nodules diameter size≥5mm, and specific nodule distribution (multiple in both lungs, multiple in the right lung or solitary in the upper or middle right lobe) were important potential predictors for malignity. In the validation set, sensitivity, specificity and AUC were 0.855, 0.833 and 0.828 (95%CI: 0.712-0.909), respectively.

Conclusion

This study conducted a clinical assessment model to differentiate benignity and malignancy of pediatric pulmonary nodules. We suggested that a nodule’s diameter, distribution and patient’s history of malignancies are predictable factors in benign or malignant determination.
Bibliometric analysis of quality of life in implant-based breast reconstruction
Authors: Daneshi Kian , Ruccia Francesca , Merh Radhika , Barlattani Tommaso , Alderhalli Raed , Clemens Mark Warren , Khajuria Ankur
Published: August 2024

Background

Implant-based breast reconstruction (IBR), following mastectomy, significantly impacts patients’ quality of life (QoL), necessitating accurate measurement through psychometrically robust patient-reported outcome measure (PROM) tools. This bibliometric analysis aims to discern trends, identify gaps, and evaluate the use of such tools in the IBR literature.

Methods

The 100 most cited publications regarding QoL in implant-based reconstruction were identified on Web of Science, across all available journal years (from 1977 to 2024) on 10 March 2024. Study details, including the citation count, main content focus, outcome measures, and usage of psychological questionnaires, were extracted and tabulated from each publication. The Oxford Centre for Evidence-Based Medicine (OCEBM) levels of evidence (LOE) of each study were assessed.

Results

The 100 most cited publications on QoL in implant-based reconstruction were identified, encompassing 64,192 patients and 28,114 reconstructed breasts. Citations per publication ranged from 62 to 457 (mean, 124.95 ± 73.05), with the highest-cited study being authored by Al-Ghazal (n = 457). The vast majority of publications were LOE II (n = 52), representative of prospective cohort studies, systematic reviews of non-randomised studies, and systematic review and meta-analysis. The number of publications for LOE V, IV, III, and I was 0, 7, 41, and 0, respectively. The main content focus was “quality of life” in 83 publications, with significant utilisation of the BREAST-Q questionnaire. A total of 80 publications used validated questionnaires with psychometric development.

Conclusions

This analysis demonstrates that the research methodologies within IBR mostly consist of moderate-quality publications; however, notably, there was a lack of LOE I studies, underscoring a gap in high-quality research within the field. Moreover, only 62/100 used validated PROM tools. Future IBR research studies should be focussed on most robust methodologies, incorporating validated PROM tools, to optimise shared-decision making and informed consent.
The potential therapeutic role of curcumin in osteoporosis treatment: based on multiple signaling pathways
Authors: Wang Keyu
Published: August 2024

Osteoporosis is a common chronic metabolic bone disease caused by disturbances in normal bone metabolism and an imbalance between osteoblasts and osteoclasts. Osteoporosis is characterized by a decrease in bone mass and bone density, leading to increased bone fragility. Osteoporosis is usually treated with medications and surgical methods, but these methods often produce certain side effects. Therefore, the use of traditional herbal ingredients for the treatment of osteoporosis has become a focus of attention and a hot topic in recent years. Curcumin, widely distributed among herbs such as turmeric, tulip, and curcuma longa, contains phenolic, terpenoid, and flavonoid components. Modern pharmacological studies have confirmed that curcumin has a variety of functions including antioxidant and anti-inflammatory properties. In addition, curcumin positively regulates the differentiation and promotes the proliferation of osteoblasts, which play a crucial role in bone formation. Multiple studies have shown that curcumin is effective in the treatment of osteoporosis as it interacts with a variety of signaling pathway targets, thereby interfering with the formation of osteoblasts and osteoclasts and regulating the development of osteoporosis. This review summarized the key signaling pathways and their mechanisms of action of curcumin in the prevention and treatment of osteoporosis and analyzed their characteristics and their relationship with osteoporosis and curcumin. This not only proves the medicinal value of curcumin as a traditional herbal ingredient but also further elucidates the molecular mechanism of curcumin’s anti-osteoporosis effect, providing new perspectives for the prevention and treatment of osteoporosis through multiple pathways.
Identification of miRNAs related to osteoporosis by high-throughput sequencing
Authors: Huang Jiachun , Yang Haolin , Chai Shuang , Lin Yanping , Zhang Zhihai , Huang Hongxing , Wan Lei
Published: August 2024

Background

Osteoporosis is a major health issue. MicroRNAs (miRNAs) play multiple roles in regulating cell growth and development. High-throughput sequencing technology is widely used nowadays.

Objective

To screen for and validate miRNAs associated with osteoporosis.

Method

Bone specimens from patients with (n = 3) and without (n = 3) osteoporosis were collected. High-throughput sequencing was used to screen for miRNAs that were then analyzed using volcano maps, Wayne maps, gene ontology (GO) analysis, and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Confirmation of the miRNAs was done using qRT-PCR.

Results

The analysis of sequencing showed that there were 12 miRNAs that were down-regulated and five miRNAs that were upregulated in osteoporosis. GO and KEGG identified these miRNAs as being associated with bone metabolism. The qRT-PCR results showed that miR-140-5p, miR-127-3p, miR-199b-5p, miR-181a-5p, miR-181d-5p, and miR-542-3p exhibited a decrease of 2.27-, 3.00-, 3.48-, 2.67-, 2.41-, and 1.98-fold (all P < 0.05) in osteoporosis compared to controls. Conversely, miR-486-3p and miR-486-5p demonstrated an increase of 2.17- and 3.89-fold (P < 0.05) (all P < 0.05).

Conclusion

This study utilized high-throughput sequencing to detect miRNAs that were expressed differently in individuals with osteoporosis. In osteoporosis, six miRNAs (miR-140-5p, miR-127-3p, miR-199b-5p, miR-181a-5p, miR-181d-5p, and miR-542) were found to have decreased expression, whereas two miRNAs (miR-486-3p and miR-486-5p) were found to have increased expression. The initial manifestation of various miRNAs might serve as predictive indicators and potentially anticipate the progression of osteoporosis.
“It's not the what but (also) the how”: characterizing left-wing populism in political texts
Authors: Raveau María Paz , Fuentes-Bravo Claudio , Fernández Miguel Ángel , Couyoumdjian Juan Pablo , del Solar María José
Published: August 2024

Despite all the elasticity and even ambiguity surrounding the concept of populism, the existing paradigms converge in the recognition of a populist rhetoric. By using Natural Language Processing (NLP) tools we propose a set of linguistic and discursive markers to identify populist markers in Presidential speeches. The performance of these markers is subsequently tested against the Global Populism Database (GPD). We set-up a multinomial regression model to study the predictive power of these markers on the GPD populist score, focusing on left-wing populist leaders in Spanish-speaking countries in Latin America. We are thus able to characterize (left-wing) populism as a style of communication, as well as to understand what is behind this rhetoric. Our results show that ingroup and emotional content are more present in populist speeches. We also find a positive relation between populism and the use future tense and conditional connectors, which suggest an intention to manipulate the audience. These results have implications both for the current understanding of (left-wing) populist rhetoric and for the conceptualization of populism itself.
What is it like to be a bot? The world according to GPT-4
Authors: Lloyd Dan
Published: August 2024

The recent explosion of Large Language Models (LLMs) has provoked lively debate about “emergent” properties of the models, including intelligence, insight, creativity, and meaning. These debates are rocky for two main reasons: The emergent properties sought are not well-defined; and the grounds for their dismissal often rest on a fallacious appeal to extraneous factors, like the LLM training regime, or fallacious assumptions about processes within the model. The latter issue is a particular roadblock for LLMs because their internal processes are largely unknown – they are colossal black boxes. In this paper, I try to cut through these problems by, first, identifying one salient feature shared by systems we regard as intelligent/conscious/sentient/etc., namely, their responsiveness to environmental conditions that may not be near in space and time. They engage with subjective worlds (“s-worlds”) which may or may not conform to the actual environment. Observers can infer s-worlds from behavior alone, enabling hypotheses about perception and cognition that do not require evidence from the internal operations of the systems in question. The reconstruction of s-worlds offers a framework for comparing cognition across species, affording new leverage on the possible sentience of LLMs. Here, we examine one prominent LLM, OpenAI’s GPT-4. Inquiry into the emergence of a complex subjective world is facilitated with philosophical phenomenology and cognitive ethology, examining the pattern of errors made by GPT-4 and proposing their origin in the absence of an analogue of the human subjective awareness of time. This deficit suggests that GPT-4 ultimately lacks a capacity to construct a stable perceptual world; the temporal vacuum undermines any capacity for GPT-4 to construct a consistent, continuously updated, model of its environment. Accordingly, none of GPT-4’s statements are epistemically secure. Because the anthropomorphic illusion is so strong, I conclude by suggesting that GPT-4 works with its users to construct improvised works of fiction.
Smoking as a mediator in the association between major depressive disorder and schizophrenia on lung cancer risk: a bidirectional/multivariable and mediation Mendelian randomization study
Authors: Zhu Xirong , Ye Ruizhi , Jiang Xianming , Zhang Jing
Published: August 2024

Background & Aims

Major depressive disorder and schizophrenia have been hypothesized to be closely associated with cancer. However, the associations between these psychiatric conditions and the development of lung cancer remain uncertain. This study aimed to explore the causal relationship among major depressive disorder, schizophrenia, and the risk of lung cancer.

Methods

Two-sample bidirectional/multivariable and mediation Mendelian randomization (MR) analyses were conducted. Genome-wide summary data on major depressive disorder (N=500,199) and schizophrenia (N=127,906) were utilized. Data on the risk of lung cancer (overall, adenocarcinoma, and squamous cell) were collected from a cohort of individuals of European ancestry (N=27,209). Three smoking-related behaviors (smoking initiation, pack years of smoking, and cigarettes smoked per day) were included in the multivariable and mediation MR analyses.

Results

Patients with schizophrenia had a significantly greater risk of developing lung cancer (odds ratio (OR) = 1.144, 95% confidence interval (95% CI): 1.048-1.248, P = 0.003). The number of cigarettes smoked per day partially mediated the relationship between schizophrenia and the overall risk of lung cancer (OR = 1.185, 95% CI: 1.112-1.264, P = 0.021, proportion of mediation effect: 61.033%). However, there is no reliable evidence indicating an association between major depressive disorder and the risk of lung cancer (overall, adenocarcinoma, and squamous cell cancer).

Conclusions

The findings indicated an association between schizophrenia and an increased risk of lung cancer, with smoking served as a partial mediator. When smoking was included in the regression analysis, the explanatory power of schizophrenia diagnosis was reduced, suggesting that smoking may be an important causal contributor to lung cancer in this population. Given the high prevalence of smoking among individuals with schizophrenia, these results underscore the need for further research to explore the underlying mechanisms of smoking’s impact. Consequently, greater emphasis should be placed on monitoring the respiratory health of individuals with schizophrenia and implementing early interventions to address smoking-related behaviors.
Understanding premenstrual exacerbation: navigating the intersection of the menstrual cycle and psychiatric illnesses
Authors: Lin Jenny , Nunez Christine , Susser Leah , Gershengoren Liliya
Published: August 2024

Premenstrual exacerbation of an existing psychiatric disorder refers to the worsening of symptoms inherent to the condition during the premenstrual phase. Research consistently indicates that hormonal fluctuations during the menstrual cycle present a unique period of vulnerability for the onset or exacerbation of psychiatric symptoms, impacting diagnosis, risk assessment, and treatment. This review sought to elucidate the phenomenon of premenstrual exacerbation and its impact across a spectrum of psychiatric illnesses, including mood, anxiety, psychotic, obsessive-compulsive, personality, and trauma-related disorders. Despite the expanded research in recent years on premenstrual dysphoric disorder and premenstrual syndrome, premenstrual exacerbation remains underexplored and poorly defined. This review offers significant contributions to the diagnosis and management of psychiatric conditions, advocating for heightened awareness and novel treatment approaches in the context of premenstrual exacerbation.
From definition to protection: dilemmas and reflections on the right to refuse treatment for patients with mental disorders in Chinese mainland
Authors: Li Xiaofu , Li Xiaofan
Published: August 2024

Background

The case of “a multimillionaire who was sent to a psychiatric hospital after an argument with his son” has sparked heated debate in the Chinese mainland. This incident is particularly significant as 2023 marks the 10th anniversary of the implementation of the Mental Health Law of the People's Republic of China. The focus of the ongoing debate, as brought to light by the aforementioned case, is centered on the right to refuse treatment for patients with mental disorders.

Methods

This paper is a post-hoc study with a systematic analysis of literature and cases. To ascertain the relationship between the right to refuse treatment for patients with mental disorders and the Mental Health Law, the authors identified key information and data from both official government websites and reliable non-governmental information.

Result

Both literature and practice have proven that the compulsory hospitalization rule under the Mental Health Law is a denial of the right to refuse treatment for patients who are compulsorily hospitalized. In the absence of changes to the law, compulsory hospitalization will inevitably lead to compulsory treatment in the Chinese mainland.

Conclusion

According to the human dignity and self-determination right established in the Constitution of the People's Republic of China, patients who are compulsorily hospitalized have the right to refuse treatment. In the absence of a change in the law, given that no neutral review mechanism has been established for such patients and their treatment in the mainland, setting up an internal review mechanism is a more feasible way of protecting the right to refuse treatment for patients with mental disorders.
Spatiotemporal analysis of tuberculosis in the Hunan Province, China, 2014–2022
Authors: Huang Guojun , Xu Zuhui , Bai Liqiong , Liu Jianjun , Yu Shicheng , Yao Hongyan
Published: August 2024

Background

Pulmonary tuberculosis (PTB) is a major infectious disease that threatens human health. China is a high tuberculosis-burden country and the Hunan Province has a high tuberculosis notification rate. However, no comprehensive analysis has been conducted on the spatiotemporal distribution of PTB in the Hunan Province. Therefore, this study investigated the spatiotemporal distribution of PTB in the Hunan Province to enable targeted control policies for tuberculosis.

Methods

We obtained data about cases of PTB in the Hunan Province notified from January 2014 to December 2022 from the China Information System for Disease Control and Prevention. Time-series analysis was conducted to analyze the trends in PTB case notifications. Spatial autocorrelation analysis was conducted to detect the spatial distribution characteristics of PTB at a county level in Hunan Province. Space-time scan analysis was conducted to confirm specific times and locations of PTB clustering.

Results

A total of 472,826 new cases of PTB were notified in the Hunan Province during the 9-year study period. The mean PTB notification rate showed a gradual, fluctuating downward trend over time. The number of PTB notifications per month showed significant seasonal variation, with an annual peak in notifications in January or March, followed by a fluctuating decline after March, reaching a trough in November or December. Moran’s I index of spatial autocorrelation revealed that the notification rate of PTB by county ranged from 0.117 to 0.317 during the study period, indicating spatial clustering. The hotspot areas of PTB were mainly concentrated in the Xiangxi Autonomous Prefecture, Zhangjiajie City, and Hengyang City. The most likely clustering region was identified in the central-southern part of the province, and a secondary clustering region was identified in the northwest part of the province.

Conclusion

This study identified the temporal trend and spatial distribution pattern of tuberculosis in the Hunan Province. PTB clustered mainly in the central-southern and northwestern regions of the province. Disease control programs should focus on strengthening tuberculosis control in these regions.
In vitro antimicrobial susceptibility of clinical isolates from adult and paediatric patients in Jordan: Antimicrobial Testing Leadership and Surveillance (ATLAS) 2010–2021
Authors: Al Jammal Dima , Bachir Julia , Moussa Jihane A. , Al Ramahi Jamal Wadi
Published: August 2024

Objectives

To evaluate the in vitro antimicrobial susceptibilities of Gram-positive and Gram-negative isolates from patients in Jordan between 2010 and 2021, through the Antimicrobial Testing Leadership and Surveillance (ATLAS) programme.

Methods

Medical centres in Jordan collected bacterial isolates from hospitalised patients with defined infection sources between 2010 and 2021 (no isolates collected in 2014). Antimicrobial susceptibility was interpreted using CLSI standards. FDA-approved breakpoints were applied for tigecycline. The identification of β-lactamase genes was performed for a proportion of isolates using multiplex PCR assays.

Results

More than 92% of Acinetobacter baumannii collected were multidrug-resistant (MDR) and/or carbapenem-resistant (CR), and > 50% susceptibility was reported only to minocycline (62.2% among both MDR and CR isolates). Rates of MDR and CR Pseudomonas aeruginosa were 14.3% and 20.5%, respectively, and among all P. aeruginosa collected from adults, susceptibility to ceftazidime/avibactam was 95.3% and to ceftolozane/tazobactam was 88.4%. For Escherichia coli from adults and MDR E. coli, susceptibility to ceftazidime/avibactam, ceftolozane/tazobactam, imipenem, meropenem and meropenem/vaborbactam was 92.1%–98.7%. Susceptibility to tigecycline was > 94% among Klebsiella pneumoniae from adult, paediatric, and ICU patients (all ages). CTX-M-15 was the most frequently identified β-lactamase gene among E. coli and K. pneumoniae. Susceptibility to most antimicrobial agents was < 50% among K. pneumoniae carrying CTX-M-15, CTX-M-9-type, NDM-5, and/or OXA-48 β-lactamase genes. All S. aureus collected were susceptible to teicoplanin, vancomycin, daptomycin, linezolid and tigecycline, with 96.1% of S. aureus from adults were susceptible to ceftaroline. Overall, 58.8% of Staphylococcus aureus were MRSA.

Conclusion

This study provides valuable information regarding antimicrobial susceptibility in Jordan between 2010 and 2021. Continued monitoring of in vitro antimicrobial susceptibility is critical in the fight against antimicrobial resistance.
Characterization of biopolymers based antibacterial films enriched with thyme essential oil and their application for milk cake preservation
Authors: Mulla Mehraj Fatema Z. , Ahmed Jasim , Vahora Aateka , Pathania Shivani , Rashed Mahmoud Said
Published: August 2024

Polysaccharide-based packaging and its ever-increasing applications in response to the need for sustainable and safe packaging have prompted researchers to investigate the development of antimicrobial biopolymer-based packaging for shelf-life enhancement of food samples. A film made by blending chitosan and pectin had good mechanical properties, that is, a tensile strength of 7.43 MPa and an elongation at break of 23.033%. To produce active packaging, thyme essential oil (TEO) was added to the blend at concentrations of 10%, 15%, and 20%. The developed film was characterized for its techno-functional and antimicrobial properties. Further the developed films were employed in an active food packaging system for milk cake. Films with added TEO showed significant enhancements in elongation at break (28.22%) and water vapor barrier properties (30.08 g/h.m2). Additionally, the microstructure of the film was also improved, as demonstrated by scanning electron microscopy (SEM) and atomic force microscopy (AFM) measurements. Fourier transform infrared spectroscopy (FTIR) indicated the interaction of blended matrix (chitosan and pectin) and TEO as observed from the formation of hydrogen bonds with the addition of TEO owing to the OH functional groups. The use of TEO in pectin and chitosan-based film and its application for milk cake packaging showed delayed microbiological contamination and hardness of milk cake samples. The shelf life of milk cake packaged in pectin and chitosan blend film with 20% TEO was enhanced by > 10 days.
Effective enhancement of the immunomodulatory capacity of canine adipose-derived mesenchymal stromal cells on colitis by priming with colon tissue from mice with colitis
Authors: Yasumura Yuyo , Teshima Takahiro , Nagashima Tomokazu , Michishita Masaki , Taira Yoshiaki , Suzuki Ryohei , Matsumoto Hirotaka
Published: August 2024

Introduction

The therapeutic efficacy of mesenchymal stromal cells (MSCs) in inflammatory bowel disease is not completely known and is not consistent. Priming with inflammatory cytokines has been proposed to adapt MSCs to an inflammatory environment to have them ready to counteract it, but may have undesirable effects on MSCs, such as increased immunogenicity. In this study, we hypothesized that priming MSCs with inflamed intestinal tissue would more effectively enhance their therapeutic effect on intestinal inflammation.

Methods

The capacity of canine adipose-derived MSCs (cADSCs) primed with colon tissue homogenates from mice with experimentally induced colitis or a combination of tumor necrosis factor-α and interferon-γ to inhibit T-cell proliferation was analyzed, along with their own apoptosis, proliferation, cell surface marker expression, and transcriptome. In addition, colitis mice were treated with the primed cADSCs to assess colitis severity and immune cell profile.

Results

Priming with cytokines induced apoptosis, decreased cell proliferation, and major histocompatibility complex-II gene expression in cADSCs, but these adverse effects were mild or absent with colitis-tissue priming. cADSCs primed with colitis tissue reduced the severity of colitis via the induction of M2 macrophages and T-regulatory cells and suppression of T-helper (Th)1/Th17-cell responses, and their effects were comparable to those of cytokine-primed cells.

Discussion

Our results emphasize the importance of the activation of MSCs by the appropriate microenvironment to maximize their therapeutic effect.
The Perception of Brazilian Livestock Regarding the Use of Precision Livestock Farming for Animal Welfare
Authors: Moreira Michele da Rocha , Trabachini Aldie , Amorim Magno do Nascimento , Harada Érik dos Santos , da Silva Marcelo Andrade , Silva-Miranda Késia Oliveira da
Published: August 2024

This study explores pig producers’ perceptions regarding the use of technologies for animal welfare in pig farming, highlighting the gap between the productive sector and academic institutions. The research was conducted through a questionnaire administered to producers from different cities in Brazil, addressing topics such as property infrastructure, technology adoption, knowledge about animal welfare, and interaction with academic institutions. The results revealed that although the majority of producers have access to information about technologies and animal welfare, there is a perceived resistance to adopting these technologies, reflected in the lack of interest in responding to academic questionnaires. The analysis also points to the influence of producers’ profiles, highlighting the importance of academic education and experience in the sector in the perception and adoption of technologies. Additionally, the research highlights the growing presence of commercial companies, filling the gap between academic research and practical application and suggesting the need for more effective strategies to engage producers in the debate on animal welfare and related technologies. These results have important implications for the development of policies and practices aimed at the sustainable advancement of livestock, encouraging greater integration and collaboration among the various actors in the sector.
Transcriptomic Analysis of Alfalfa Flowering and the Dual Roles of MsAP1 in Floral Organ Identity and Flowering Time
Authors: Jiang Xu , Cui Huiting , Wang Zhen , Long Ruicai , Yang Qingchuan , Kang Junmei
Published: August 2024

Flowering, the transition from the vegetative to the reproductive stage, is vital for reproductive success, affecting forage quality, the yield of aboveground biomass, and seed production in alfalfa. To explore the transcriptomic profile of alfalfa flowering transition, we compared gene expression between shoot apices (SAs) at the vegetative stage and flower buds (FBs) at the reproductive stage by mRNA sequencing. A total of 3,409 DEGs were identified, and based on gene ontology (GO), 42.53% of the most enriched 15 processes were associated with plant reproduction, including growth phase transition and floral organ development. For the former category, 79.1% of DEGs showed higher expression levels in SA than FB, suggesting they were sequentially turned on and off at the two test stages. For the DEGs encoding the components of circadian rhythm, sugar metabolism, phytohormone signaling, and floral organ identity genes, 60.71% showed higher abundance in FB than SA. Among them, MsAP1, an APETALA1 (AP1) homolog of Arabidopsis thaliana, showed high expression in flower buds and co-expressed with genes related to flower organ development. Moreover, ectopic expression of MsAP1 in Arabidopsis resulted in dwarfism and early flowering under long-day conditions. The MsAP1-overexpression plant displayed morphological abnormalities including fused whorls, enlarged pistils, determinate inflorescence, and small pods. In addition, MsAP1 is localized in the nucleus and exhibits significant transcriptional activity. These findings revealed a transcriptional regulation network of alfalfa transition from juvenile phase to flowering and provided genetic evidence of the dual role of MsAP1 in flowering and floral organ development.
ShinyAnonymizer Enhanced Version and Beyond: A Further Exploration of Privacy-Preserving Solutions in Health Data Management
Authors: Vardalachakis Marios , Papadakis Nikos , Tampouratzis Manolis
Published: August 2024

Healthcare institutions generate massive amounts of valuable patient data in the digital age. Finding the right balance between patient privacy and the demand for data-driven medical enhancements is essential. Since data privacy has become increasingly important, robust technologies must be developed to safeguard private data and allow meaningful exploration. This issue was addressed by ShinyAnonymizer, which was first created to anonymize health data. It achieves this by rendering anonymization methods easily available to users. The enhanced version of ShinyAnonymizer, with an essential improvement in performance, is presented in this study. We explain the merging of data analysis, visualization, and privacy-focused statistics paradigms with data anonymization, hashing, and encryption, offering researchers and data analysts an extensive collection of tools for trustworthy data management.
Analysis of Solid Backfilling Effects on Strata and Ground Subsidence in a Longwall Coal Mine Beneath a City
Authors: Deon Makavelo , Zhang Qiang , Li Meng , Huang Peng , Wu Zhongya , Francisco Chano
Published: August 2024

The solid backfilling mining method is one of the methods used to solve problems arising from strata and ground subsidence in underground mines. Through 2D physical analog modeling, 3D numerical simulation, and field measurement, the effects of the solid backfilling method were analyzed, providing a better insight into optimizing the configurations of a working face beneath a city for safety, environmental problems, and its use in production. In the physical modeling, MatchID software was employed to capture the movement characteristics of overlying strata and ground subsidence during mining and backfilling. Key parameters such as vertical displacement, subsidence characteristics, and rock mass stress variations were monitored and analyzed. In the numerical simulation, FLAC3D was used to simulate and analyze the effect of the backfill body on strata and ground subsidence above the backfill working face. For the field measurements, the Continuously Operating Reference Station (CORS) system was used to confirm the effective control of ground subsidence. With a filling ratio of 80%, the three methods are consistent and show a maximum subsidence value of 0.46 mm (physical simulation), 50.4 mm (numerical simulation), and 47 mm (experienced), significantly lower than the predicted subsidence, which is 281 mm. Therefore, this study demonstrates the reliability and scientific validity of both the physical analog modeling method and the field measurement method in measuring the efficiency of solid backfilling, providing valuable insights into strata and ground subsidence control in longwall coal mining.
Thermal Performance Analysis of Aluminum Alloy Phase Change Panels for Regions with Hot Summers and Warm Winters
Authors: Jiang Baoshi , Yang Chen
Published: August 2024

Utilizing phase change materials (PCMs) in passive energy-saving wall panels to regulate indoor temperatures during hot seasons can improve people’s thermal comfort and reduce the energy consumption of air conditioning systems. This study is based on the hot summer and warm winter climatic characteristics of Hainan. According to local meteorological data and residents’ living habits, a suitable phase change temperature of approximately 28 °C was determined. A composite PCM of paraffin and stearic acid n-butyl ester was prepared and tested for thermal performance. Encased in an aluminum box with non-penetrating aluminum rods to enhance heat transfer, the phase change panel was applied to the inner side of exterior walls. Thermal tests demonstrated that increasing the mass ratio of stearic acid n-butyl ester to paraffin lowers the melting point and latent heat. At a 3:7 mass ratio, the melting point of the composite PCM was 28.30 °C, and the latent heat was 128.26 J/g. The 20 mm thick panel maintained a stable phase change process, with unheated surface temperatures between 28 °C and 29 °C for up to 180 min. Compared to panels without aluminum rods, those with rods exhibited a 20% longer phase change time, extended heat transfer paths, and reduced liquid-phase convective heat transfer rates, demonstrating improved PCM utilization. Therefore, the phase change panel with non-penetrating aluminum rods exhibits excellent insulation and temperature control properties.
Proposal of a Design Procedure for Steel Frames with Viscoelastic Dampers
Authors: Bosco Melina , Floridia Andrea , Rossi Pier Paolo
Published: August 2024

The effectiveness of viscoelastic dampers as passive control devices has been demonstrated in the past through both experimental and numerical investigations. Based on the Modal Strain Energy Method, some authors have also proposed design procedures to size the viscoelastic dampers assuming a fist-mode behavior of the structure. However, even if the damped structure is governed by the first mode of vibration, viscoelastic dampers are sensitive to the frequencies of the upper modes and transmit unexpected internal forces to braces. This paper aims to develop a simple design procedure for steel moment-resisting frames equipped with viscoelastic dampers considering the effects of the higher modes of vibrations on the internal forces transmitted from the dampers to the braces. In the perspective of a designer-oriented study, the seismic demand is evaluated through simple analytical tools, such as the lateral force method or the response spectrum analysis. The design procedure is applied to a set of steel moment-resisting frames considering two levels of seismic hazard and two types of soil. Finally, the effectiveness of the proposed procedure is verified through nonlinear dynamic analysis. Based on the results, it is found that the proposed design procedure ensures the control of the story drift below prefixed limits and to predict accurately the internal forces that arise in the braces.