bluuebunny/crossref_metadata_2025 huggingface dataset:
Alterations of lipid-related genes during anti-tuberculosis treatment: insights into host immune responses and potential transcriptional biomarkers
Authors: Phat Nguyen Ky , Tien Nguyen Tran Nam , Anh Nguyen Ky , Yen Nguyen Thi Hai , Lee Yoon Ah , Trinh Hoang Kim Tu , Le Kieu-Minh , Ahn Sangzin , Cho Yong-Soon , Park Seongoh , Kim Dong Hyun , Long Nguyen Phuoc , Shin Jae-Gook
Published: October 2023

Background

The optimal diagnosis and treatment of tuberculosis (TB) are challenging due to underdiagnosis and inadequate treatment monitoring. Lipid-related genes are crucial components of the host immune response in TB. However, their dynamic expression and potential usefulness for monitoring response to anti-TB treatment are unclear.

Methodology

In the present study, we used a targeted, knowledge-based approach to investigate the expression of lipid-related genes during anti-TB treatment and their potential use as biomarkers of treatment response.

Results and discussion

The expression levels of 10 genes (ARPC5, ACSL4, PLD4, LIPA, CHMP2B, RAB5A, GABARAPL2, PLA2G4A, MBOAT2, and MBOAT1) were significantly altered during standard anti-TB treatment. We evaluated the potential usefulness of this 10-lipid-gene signature for TB diagnosis and treatment monitoring in various clinical scenarios across multiple populations. We also compared this signature with other transcriptomic signatures. The 10-lipid-gene signature could distinguish patients with TB from those with latent tuberculosis infection and non-TB controls (area under the receiver operating characteristic curve > 0.7 for most cases); it could also be useful for monitoring response to anti-TB treatment. Although the performance of the new signature was not better than that of previous signatures (i.e., RISK6, Sambarey10, Long10), our results suggest the usefulness of metabolism-centric biomarkers

Conclusions

Lipid-related genes play significant roles in TB pathophysiology and host immune responses. Furthermore, transcriptomic signatures related to the immune response and lipid-related gene may be useful for TB diagnosis and treatment monitoring.
Determination of the target of monoclonal immunoglobulins: a novel diagnostic tool for individualized MGUS therapy, and prevention and therapy of smoldering and multiple myeloma
Authors: Hermouet Sylvie , Bigot-Corbel Edith , Harb Jean
Published: October 2023

Subsets of patients diagnosed with a monoclonal gammopathy of undetermined significance (MGUS), smoldering multiple myeloma (SMM) or multiple myeloma (MM), present with a monoclonal immunoglobulin (Ig) specific for an infectious pathogen, including hepatitis C and B viruses (HCV, HBV), Helicobacter pylori and several Herpesviruses. Such cases are likely initiated by infection, since in the context of HCV- or HBV-infected patients, antiviral therapy can lead to the disappearance of antigenic stimulation, control of clonal plasma cells, and reduced or suppressed monoclonal Ig production. Complete remission has been obtained with anti-HCV therapy in refractory MM with a HCV-specific monoclonal Ig, and antiviral treatments significantly improved the probability of survival of MM patients infected with HCV or HBV prior to the diagnosis of MM. Monoclonal Igs may also target glucolipids, particularly glucosylsphingosine (GlcSph), and GlcSph-reducing therapy can lead to complete remission in SMM and MM patients presenting with a GlcSph-specific monoclonal Ig. The present review describes the importance of determining the target of the monoclonal Ig of MGUS, SMM and MM patients, and discusses the efficacy of target-reducing treatments in the management of MGUS, SMM and MM cases who present with a monoclonal Ig reactive against a treatable infectious pathogen or GlcSph.
Adenosine, bridging chronic inflammation and tumor growth
Authors: Chen Luxia , Alabdullah Mohamad , Mahnke Karsten
Published: October 2023

Adenosine (Ado) is a well-known immunosuppressive agent that may be released or generated extracellularly by cells, via degrading ATP by the sequential actions of the ectonucleotides CD39 and CD73. During inflammation Ado is produced by leukocytes and tissue cells by different means to initiate the healing phase. Ado downregulates the activation and the effector functions of different leukocyte (sub-) populations and stimulates proliferation of fibroblasts for re-establishment of intact tissues. Therefore, the anti-inflammatory actions of Ado are already intrinsically triggered during each episode of inflammation. These tissue-regenerating and inflammation-tempering purposes of Ado can become counterproductive. In chronic inflammation, it is possible that Ado-driven anti-inflammatory actions sustain the inflammation and prevent the final clearance of the tissues from possible pathogens. These chronic infections are characterized by increased tissue damage, remodeling and accumulating DNA damage, and are thus prone for tumor formation. Developing tumors may further enhance immunosuppressive actions by producing Ado by themselves, or by “hijacking” CD39+/CD73+ cells that had already developed during chronic inflammation. This review describes different and mostly convergent mechanisms of how Ado-induced immune suppression, initially induced in inflammation, can lead to tumor formation and outgrowth.
Multi-omics analyses unravel DNA damage repair-related clusters in breast cancer with experimental validation
Authors: Liu Peng , Deng Xinpei , Zhou Huamao , Xie Jindong , Kong Yanan , Zou Yutian , Yang Anli , Li Xing
Published: October 2023

Background

As one of the most common malignancies worldwide, breast cancer (BC) exhibits high heterogeneity of molecular phenotypes. The evolving view regarding DNA damage repair (DDR) is that it is context-specific and heterogeneous, but its role in BC remains unclear.

Methods

Multi-dimensional data of transcriptomics, genomics, and single-cell transcriptome profiling were obtained to characterize the DDR-related features of BC. We collected 276 DDR-related genes based on the Molecular Signature Database (MSigDB) database and previous studies. We acquired public datasets included the SCAN-B dataset (GEO: GSE96058), METABRIC database, and TCGA-BRCA database. Corresponding repositories such as transcriptomics, genomics, and clinical information were also downloaded. We selected scRNA-seq data from GEO: GSE176078, GSE114727, GSE161529, and GSE158724. Bulk RNA-seq data from GEO: GSE176078, GSE18728, GSE5462, GSE20181, and GSE130788 were extracted for independent analyses.

Results

The DDR classification was constructed in the SCAN-B dataset (GEO: GSE96058) and METABRIC database, Among BC patients, there were two clusters with distinct clinical and molecular characteristics: the DDR-suppressed cluster and the DDR-active cluster. A superior survival rate is found for tumors in the DDR-suppressed cluster, while those with the DDR-activated cluster tend to have inferior prognoses and clinically aggressive behavior. The DDR classification was validated in the TCGA-BRCA cohort and shown similar results. We also found that two clusters have different pathway activities at the genomic level. Based on the intersection of the different expressed genes among these cohorts, we found that PRAME might play a vital role in DDR. The DDR classification was then enabled by establishing a DDR score, which was verified through multilayer cohort analysis. Furthermore, our results revealed that malignant cells contributed more to the DDR score at the single-cell level than nonmalignant cells. Particularly, immune cells with immunosuppressive properties (such as FOXP3+ CD4+ T cells) displayed higher DDR scores among those with distinguishable characteristics.

Conclusion

Collectively, this study performs general analyses of DDR heterogeneity in BC and provides insight into the understanding of individualized molecular and clinicopathological mechanisms underlying unique DDR profiles.
Inactivated vaccine with glycyrrhizic acid adjuvant elicits potent innate and adaptive immune responses against foot-and-mouth disease
Authors: Shin Seokwon , Kim Hyeong Won , Ko Mi-Kyeong , Park So Hui , Kim Su-Mi , Park Jong-Hyeon , Lee Min Ja
Published: October 2023

Background

Foot-and-mouth disease (FMD) is an extremely contagious viral disease that is fatal to young animals and is a major threat to the agricultural economy by reducing production and limiting the movement of livestock. The currently commercially-available FMD vaccine is prepared using an inactivated viral antigen in an oil emulsion, with aluminum hydroxide [Al(OH)3] as an adjuvant. However, oil emulsion-based options possess limitations including slow increases in antibody titers (up to levels adequate for defense against viral infection) and risks of local reactions at the vaccination site. Further, Al(OH)3 only induces a T helper 2 (Th2) cell response. Therefore, novel adjuvants that can address these limitations are urgently needed. Glycyrrhizic acid (extracted from licorice roots) is a triterpenoid saponin and has great advantages in terms of price and availability.

Methods

To address the limitations of the currently used commercial FMD vaccine, we added glycyrrhizic acid as an adjuvant (immunostimulant) to the FMD bivalent (O PA2 + A YC) vaccine. We then evaluated its efficacy in promoting both innate and adaptive (cellular and humoral) immune reactions in vitro [using murine peritoneal exudate cells (PECs) and porcine peripheral blood mononuclear cells (PBMCs)] and in vivo (using mice and pigs).

Results

Glycyrrhizic acid has been revealed to induce an innate immune response and enhance early, mid-, and long-term immunity. The studied bivalent vaccine with glycyrrhizic acid increased the expression of immunoregulatory genes such as pattern-recognition receptors (PRRs), cytokines, transcription factors, and co-stimulatory molecules.

Conclusion

Collectively, glycyrrhizic acid could have utility as a novel vaccine adjuvant that can address the limitations of commercialized FMD vaccines by inducing potent innate and adaptive immune responses.
Advancements in dendritic cell vaccination: enhancing efficacy and optimizing combinatorial strategies for the treatment of glioblastoma
Authors: Subtirelu Robert C. , Teichner Eric M. , Ashok Arjun , Parikh Chitra , Talasila Sahithi , Matache Irina-Mihaela , Alnemri Ahab G. , Anderson Victoria , Shahid Osmaan , Mannam Sricharvi , Lee Andrew , Werner Thomas , Revheim Mona-Elisabeth , Alavi Abass
Published: October 2023

Glioblastomas (GBM) are highly invasive, malignant primary brain tumors. The overall prognosis is poor, and management of GBMs remains a formidable challenge, necessitating novel therapeutic strategies such as dendritic cell vaccinations (DCVs). While many early clinical trials demonstrate an induction of an antitumoral immune response, outcomes are mixed and dependent on numerous factors that vary between trials. Optimization of DCVs is essential; the selection of GBM-specific antigens and the utilization of 18F-fludeoxyglucose Positron Emission Tomography (FDG-PET) may add significant value and ultimately improve outcomes for patients undergoing treatment for glioblastoma. This review provides an overview of the mechanism of DCV, assesses previous clinical trials, and discusses future strategies for the integration of DCV into glioblastoma treatment protocols. To conclude, the review discusses challenges associated with the use of DCVs and highlights the potential of integrating DCV with standard therapies.
Luteolin inhibits GPVI-mediated platelet activation, oxidative stress, and thrombosis
Authors: Ye Yujia , Yang Lihong , Leng Min , Wang Qian , Wu Jiankui , Wan Wen , Wang Huawei , Li Longjun , Peng Yunzhu , Chai Shengjie , Meng Zhaohui
Published: October 2023

Introduction: Luteolin inhibits platelet activation and thrombus formation, but the mechanisms are unclear. This study investigated the effects of luteolin on GPVI-mediated platelet activation in vitro and explored the effect of luteolin on thrombosis, coagulation, and platelet production in vivo.

Methods: Washed human platelets were used for aggregation, membrane protein expression, ATP, Ca2+, and LDH release, platelet adhesion/spreading, and clot retraction experiments. Washed human platelets were used to detect collagen and convulxin-induced reactive oxygen species production and endogenous antioxidant effects. C57BL/6 male mice were used for ferric chloride-induced mesenteric thrombosis, collagen-epinephrine induced acute pulmonary embolism, tail bleeding, coagulation function, and luteolin toxicity experiments. The interaction between luteolin and GPVI was analyzed using solid phase binding assay and surface plasmon resonance (SPR).

Results: Luteolin inhibited collagen- and convulxin-mediated platelet aggregation, adhesion, and release. Luteolin inhibited collagen- and convulxin-induced platelet ROS production and increased platelet endogenous antioxidant capacity. Luteolin reduced convulxin-induced activation of ITAM and MAPK signaling molecules. Molecular docking simulation showed that luteolin forms hydrogen bonds with GPVI. The solid phase binding assay showed that luteolin inhibited the interaction between collagen and GPVI. Surface plasmon resonance showed that luteolin bonded GPVI. Luteolin inhibited integrin αIIbβ3-mediated platelet activation. Luteolin inhibited mesenteric artery thrombosis and collagen- adrenergic-induced pulmonary thrombosis in mice. Luteolin decreased oxidative stress in vivo. Luteolin did not affect coagulation, hemostasis, or platelet production in mice.

Discussion: Luteolin may be an effective and safe antiplatelet agent target for GPVI. A new mechanism (decreased oxidative stress) for the anti-platelet activity of luteolin has been identified.
Comparing modeling methods of genomic prediction for growth traits of a tropical timber species, Shorea macrophylla
Authors: Akutsu Haruto , Na’iem Mohammad , Widiyatno  , Indrioko Sapto , Sawitri  , Purnomo Susilo , Uchiyama Kentaro , Tsumura Yoshihiko , Tani Naoki
Published: October 2023

Introduction

Shorea macrophylla is a commercially important tropical tree species grown for timber and oil. It is amenable to plantation forestry due to its fast initial growth. Genomic selection (GS) has been used in tree breeding studies to shorten long breeding cycles but has not previously been applied to S. macrophylla.

Methods

To build genomic prediction models for GS, leaves and growth trait data were collected from a half-sib progeny population of S. macrophylla in Sari Bumi Kusuma forest concession, central Kalimantan, Indonesia. 18037 SNP markers were identified in two ddRAD-seq libraries. Genomic prediction models based on these SNPs were then generated for diameter at breast height and total height in the 7th year from planting (D7 and H7).

Results and discussion

These traits were chosen because of their relatively high narrow-sense genomic heritability and because seven years was considered long enough to assess initial growth. Genomic prediction models were built using 6 methods and their derivatives with the full set of identified SNPs and subsets of 48, 96, and 192 SNPs selected based on the results of a genome-wide association study (GWAS). The GBLUP and RKHS methods gave the highest predictive ability for D7 and H7 with the sets of selected SNPs and showed that D7 has an additive genetic architecture while H7 has an epistatic genetic architecture. LightGBM and CNN1D also achieved high predictive abilities for D7 with 48 and 96 selected SNPs, and for H7 with 96 and 192 selected SNPs, showing that gradient boosting decision trees and deep learning can be useful in genomic prediction. Predictive abilities were higher in H7 when smaller number of SNP subsets selected by GWAS p-value was used, However, D7 showed the contrary tendency, which might have originated from the difference in genetic architecture between primary and secondary growth of the species. This study suggests that GS with GWAS-based SNP selection can be used in breeding for non-cultivated tree species to improve initial growth and reduce genotyping costs for next-generation seedlings.
Psychometric properties of the Chinese version of Sport Anxiety Scale-2
Authors: Zhang Jinrui , Zhang Zhiwen , Peng Shuo , Veloo Arsaythamby , Bailey Richard Peter , Tan Wee Hoe
Published: October 2023

Introduction

The Sport Anxiety Scale-2 (SAS-2) is a validated measure of sports trait anxiety, with promising psychometric properties. However, its cross-cultural applicability in Chinese samples remains unexplored. Thus, the primary objectives of this study were twofold: to translate the SAS-2 into Chinese and assess the psychometric properties of the Chinese version.

Methods

In Study 1, we initiated the translation of the SAS-2 into Chinese. This assessment involved bilingual Chinese students proficient in both English and Chinese. Additionally, we conducted a cross-linguistic measurement invariance analysis. In Study 2, we delved into the psychometric properties of the Chinese SAS-2 using a sample of Chinese student athletes. This examination encompassed an evaluation of its factor structure, convergent and discriminant validity, and measurement invariance across genders.

Results

Our findings in Study 1 indicated no significant differences in item scores between the Chinese SAS-2 and the English version, and measurement invariance across languages. In Study 2, we uncovered that the Chinese SAS-2 and its factors exhibited excellent reliability, with Cronbach’s alpha values exceeding 0.80. Confirmatory factor analyses upheld the original three-factor model, demonstrating acceptable model fit indices (CFI = 0.96, TLI = 0.93, RMSEA = 0.08). Furthermore, all three factors of the Chinese SAS-2 displayed significant and positive correlations with athlete burnout and State-Trait anxiety. Additionally, this study elucidated the mediating role of Concentration Disruption (Somatic anxiety and Concentration Disruption) in the relationship between the Trait (State) anxiety, and athlete burnout. Moreover, we identified measurement invariance of the Chinese version of the SAS-2 across genders. Finally, female college athletes exhibited significantly higher scores in somatic anxiety and worry compared to their male counterparts.

Discussion

In sum, our findings affirm that the Chinese version of the SAS-2 demonstrates robust reliability and correlates effectively with related criteria, thus validating its suitability for use in a Chinese context.
A comparison of dimensional and discrete models for the representation of perceived and induced affect in response to short musical sounds
Authors: Korsmit Iza Ray , Montrey Marcel , Wong-Min Alix Yok Tin , McAdams Stephen
Published: October 2023

Introduction

In musical affect research, there is considerable discussion on the best method to represent affective response. This discussion mainly revolves around the dimensional (valence, tension arousal, energy arousal) and discrete (anger, fear, sadness, happiness, tenderness) models of affect. Here, we compared these models' ability to capture self-reported affect in response to short, affectively ambiguous sounds.

Methods

In two online experiments (n1 = 263, n2 = 152), participants rated perceived and induced affect in response to single notes (Exp 1) and chromatic scales (Exp 2), which varied across instrument family and pitch register. Additionally, participants completed questionnaires measuring pre-existing mood, trait empathy, Big-Five personality, musical sophistication, and musical preferences.

Results

Rater consistency and agreement were high across all affect scales. Correlation and principal component analyses showed that two dimensions or two affect categories captured most of the variation in affective response. Canonical correlation and regression analyses also showed that energy arousal varied in a manner that was not captured by discrete affect ratings. Furthermore, all sources of individual differences were moderately correlated with all affect scales, particularly pre-existing mood and dimensional affect.

Discussion

We conclude that when it comes to single notes and chromatic scales, the dimensions of valence and energy arousal best capture the perceived and induced affective response to affectively ambiguous sounds, although the role of individual differences should also be considered.
The role of teachers' socio-emotional competence in reducing burnout through increased work engagement
Authors: Ornaghi Veronica , Conte Elisabetta , Cavioni Valeria , Farina Eleonora , Pepe Alessandro
Published: October 2023

Introduction

Research has highlighted the relevance of socio-emotional competence in buffering the harmful impacts of perceived stress on the psychological facets of the teaching profession. The purpose of this paper is to innovatively investigate the relationships between perceived stress, work engagement, and burnout in a single comprehensive model, when considering the potential role that socio-emotional competence plays in mitigating the adverse impact of perceived stress on burnout.

Methods

A total of 276 Italian in-service teachers (mean age = 46.6 ± 9.9 years) completed quantitative self-report measures of perceived stress, socio-emotional competence, work engagement, and burnout. Data were analyzed by using a structural equation modeling (SEM) approach.

Results

All fit indexes supported the model's full acceptance and suggested that teachers' socio-emotional competence reduced the effect of perceived stress on the risk of burnout by increasing their level of work engagement.

Discussion

The implications of the findings are discussed in terms of promoting interventions that target not only stress reduction but also foster teachers' socio-emotional competence in order to maintain a good level of work engagement and reduce the effect of stress on burnout.
Motivational characteristics of recreational drug use among emerging adults in social settings: an integrative literature review
Authors: Lojszczyk Alicja , Wilson Rhonda , Wood Jessica , Hutton Alison
Published: October 2023

Introduction

Recreational drug use by emerging adults has been identified as an increasingly normalized trend in social contexts. It has been documented that the consumption of these substances regularly occurs at music festivals, raves, nightlife and party settings. While it is known that emerging adults participate in these risk-taking behaviors, what is not known is their motivational characteristics for use. The aim of this review to identify and review literature describing the motivations for recreational drug use and drug choice (excluding alcohol, cannabis and tobacco) by emerging adults in social settings to inform selection of appropriately aligned harm reduction education and health messaging interventions.

Methods

Whittemore and Knafl’s (2005) integrative approach was used to conduct the review. This integrative review was based on a three-step search strategy identifying 2,772 articles published between 2000 and 2022. Eleven studies were included in the review. This review explores the following areas: drug use settings, concurrent drug use, consumer drug knowledge, motives of use including likes and dislikes and peer influence.

Results

A range of factors influence motivations of emerging adults to participate in recreational drug use. Similar to the consumption of alcohol, the use of recreational drugs by emerging adults is motivated by their perceived benefits and personal motivations to achieve euphoria, emotional intimacy, social benefits, peer influence, increased confidence and to decrease inhibitions. The review findings suggest that motivational factors that reinforce recreational drug use correlate with the desire to break away from the mundane by seeking pleasure and for the opportunity to create novel experiences. Beliefs about the positive and negative impacts of drug use, together with the desire to achieve emotional satisfaction influence drug taking activity.

Conclusion

Recreational drug use has become an increased societal norm amongst drug using peer groups and cannot be entirely prevented. It is to be noted that emerging adults have a basic understanding concerning recreational drugs, however, consumer drug knowledge and interventions that target illicit substances is lacking and should be addressed in future research. Festivals, raves and nightlife settings provide opportunity to implement health promotion as it reaches large number of vulnerable individuals in a short period of time.
NorthBEAT: exploring the service needs of youth experiencing early psychosis in Northern Ontario
Authors: Cheng Chiachen , Nadin Shevaun , Bohonis Hafsa , Katt Mae , Dewa Carolyn S.
Published: October 2023

Introduction

Early Psychosis Intervention (EPI) is critical for best outcomes. Among 369 diseases, psychosis is among those causing the greatest disability. Evidence-based interventions for youth in early stages of psychosis (EPI programs) have prevented chronic disability. Yet, EPI is frequently inaccessible for youth living in rural communities. Moreover, Indigenous youth often face more precipitous situations given inadequate staffing, and culturally unsafe care. The NorthBEAT (Barriers to Early Assessment and Treatment) project sought to understand the service needs of youth with psychosis in Northern Ontario. The goals were: (1) to describe the mental health of a subset of adolescents receiving EPI care; (2) examine Indigenous youth as a significant and vulnerable population; (3) to understand the barriers and facilitators for Indigenous and non-Indigenous youth receiving EPI.

Methods

Mixed methods (structured and narrative interviews) included: psychometric scales interviews with youth, and narrative interviews with youth, their family, and service providers Data validation workshops were held with participants.

Results

Structured interviews with 26 youth (M = 17 years) found the participants functioning moderately well with duration of untreated psychosis ranging from 1 to 96 months (M = 26 months). No significant differences were found in functioning or duration of psychosis between Indigenous and non-Indigenous youth. Narrative interviews were conducted with 18 youth, 11 family members, and 14 service providers. Identified barriers were a lack of knowledge about psychosis among service providers, a disconnected system leading to delays in treatment, help not wanted by youth, expansive geographical context. Service needs were: finding the right point of access, support for families, pre-crisis intervention, reduced stigma for youth and their families, and an EPI approach to care.

Discussion

Rural and northern youth face similar barriers to accessing EPI as urban youth. However, northern youth face additional unique challenges due to expansive geographical context, limited resources and lack of knowledge about services.
Developmental activities of elite junior hockey players: an analysis of early sport specialization
Authors: Garland W. J. , Smith K. L. , Dixon J. C. , Horton S.
Published: October 2023

Early sport specialization is a popular and contentious topic in the scientific literature and popular media. The lure of extrinsic rewards has led to increasing rates of specialization among young athletes, while expert recommendations promote multisport participation. The purpose of this study was to describe and analyze developmental activities of a group of elite junior hockey players in Canada. Within this context, elements of specialization were investigated in accordance with existing theoretical frameworks and long-term athlete development models to enhance the literature. Fifteen participants from the Ontario Hockey League completed quantitative retrospective interviews, detailing past sport and recreational activities. Thirty-one developmental milestones were assessed. Accumulated hours of activity were categorized in accordance with Côté's (1999) Developmental Model of Sports Participation, along with the number and types of sports in which they participated during childhood. Jayanthi et al.'s (2015) continuum was utilized to determine the age at which the athletes became moderately and highly specialized. Accrued hours of deliberate practice reported by participants increased from ages 6 to 16 years, as did competition in organized hockey games. Reported hours of deliberate play peaked at 9 years of age and decreased thereafter. Participants played a combined 16 sports other than hockey, ranging from an average of 2.0 at age 6, to a maximum average of 5.6 at 12 years old, and decreasing each year to 2.3 by age 15. The greatest number of hours in other sports was accumulated at 12 years of age. Using a three-point scale, participants considered themselves “highly specialized” at 14 years old; however, other quantitative indicators suggested this may have occurred at 12 years of age. Relative to previous research on early sport specialization, participants in this study spent more time practicing hockey, while ceasing hockey-specific play and other sports at younger ages. Despite a diverse sport history, hockey competition was initiated earlier than recommended, showing high levels of sport commitment as young as 9 years old. The early specialization path remains a popular trajectory among coaches, parents, and athletes in Canadian ice hockey.
Chemotherapy for the treatment of intracranial glioma in dogs
Authors: José-López Roberto
Published: October 2023

Gliomas are the second most common primary brain tumor in dogs and although they are associated with a poor prognosis, limited data are available relating to the efficacy of standard therapeutic options such as surgery, radiation and chemotherapy. Additionally, canine glioma is gaining relevance as a naturally occurring animal model that recapitulates human disease with fidelity. There is an intense comparative research drive to test new therapeutic approaches in dogs and assess if results translate efficiently into human clinical trials to improve the poor outcomes associated with the current standard-of-care. However, the paucity of data and controversy around most appropriate treatment for intracranial gliomas in dogs make comparisons among modalities troublesome. To further inform therapeutic decision-making, client discussion, and future studies evaluating treatment responses, the outcomes of 127 dogs with intracranial glioma, either presumed (n = 49) or histologically confirmed (n = 78), that received chemotherapy as leading or adjuvant treatment are reviewed here. This review highlights the status of current chemotherapeutic approaches to intracranial gliomas in dogs, most notably temozolomide and lomustine; areas of novel treatment currently in development, and difficulties to consensuate and compare different study observations. Finally, suggestions are made to facilitate evidence-based research in the field of canine glioma therapeutics.
Inequalities in Diabetes Mortality Between Microregions in Hungary
Authors: Kollányi Zsófia , Bálint Lajos , Susovits Kitti , Csépe Péter , Kovács Katalin
Published: October 2023

Objectives: Regional differences in diabetes mortality are high in Hungary. In our cross-sectional study, we aim to reveal the drivers of the inequalities in diabetes mortality across the 197 microregions of Hungary. To account for the influence of changes in healthcare and social conditions, we compared two periods (2009–12 and 2013–16).

Methods: Traditional and re-conceptualized deprivation- and healthcare provison measures were used in OLS regression models.

Results: Microregions with a high proportion of population living in “service deserts,” especially in regard to the lack of grocery stores, suffer the highest rates of diabetes mortality. Alcohol-related mortality has been proven to be a similarly and surprisingly strong predictor of diabetes mortality.

Conclusion: Food provision should be supported in areas characterized by low service density, and alcohol policy should be strengthened and targeted.
Importance and Potential of European Cross-Border Deceased Donor Organ Allocation Through FOEDUS-EOEO Platform
Authors: Elmer Andreas , Lütolf Vera Valérie , Carella Claudia , Beyeler Franziska , Krügel Nathalie , Straka Libor , Cardillo Massimo , Immer Franz
Published: October 2023

The FOEDUS-EOEO platform was relaunched in 2015 to allocate deceased donor organs across European borders when there are no suitable recipients in the donor’s country. We analyzed organ offers from 01.06.2015–31.12.2021 and present the number of offers and transplants, and utilization as percentage of transplanted organs. 1,483 organs were offered, 287 were transplanted (19.4% utilization). Yearly number of offers and transplants increased from 2017 to 2021, while utilization stabilized after 2018. Utilization was highest for organs offered by Slovakia (47.2%), followed for organs offered by Lithuania, France, Greece, and Czechia (19.3%–22.9%). The most frequently offered organ was the heart (n = 405; 27.3%), followed by the lungs (n = 369; 24.9%) and the liver (n = 345; 23.3%). Utilization differed significantly by organ type (highest for liver, 35.7%; followed by heart, 18.8%; and kidney, 18.3%) and by donor age (highest for 1 to 5 year-old donors (25.0%)). FOEDUS-EOEO allowed for many European patients receiving a long-awaited transplant, especially for very young pediatric patients waiting for a liver, a heart, or a kidney. The increasing number of participating countries has increased both the number of offered organs and, to a lesser extent, the number of transplanted organs.
The Agritourism Value Chain: An Application to the Dehesa Areas of Extremadura
Authors: Ruiz-Labrador Enrique-Eugenio , Sánchez-Martín José-Manuel , Gurría-Gascón José-Luis
Published: October 2023

The serious socioeconomic problems faced by rural environments in general, and the agricultural sector in particular, in peripheral European territories since the end of the last century have led to depopulation phenomena, the abandonment of unique traditional uses, and the loss of cultural identity. The promotion of a well-understood agrotourism sector in these territories, as an income-diversifying activity that promotes a more sustainable tourism, is a measure widely applauded by the scientific community, which seeks to curb such problems and offer an opportunity to the inhabitants who are less and less dedicated to the much maltreated primary sector. However, it is crucial to know in a personalized way the value chain associated with agrotourism and in particular that linked to the Extremadura dehesa, especially when this has not traditionally been considered a tourist resource. This paper presents a recent bibliographical review regarding the potential of the Extremadura dehesa as a viable agrotourism resource. The lack of knowledge that the tourist offer has about agrotourism and the dehesa as a business niche and diversification of income, or the gap between some activities and the appropriate channels of the value chain, are some remarkable conclusions, coinciding with those of other authors and similar territories.
Response of Soil Respiration to Altered Snow Cover in a Typical Temperate Grassland in China
Authors: Liu Yanqi , Peng Qin
Published: October 2023

The snow cover in temperate areas is undergoing significant changes, which may affect soil respiration (Rs), the second largest carbon flux in global carbon cycling. However, currently, there are relatively few in situ field studies on the effects of altered snow cover on Rs in temperate areas during the non-growing season compared to the research on Rs during the growing season. Therefore, it limited the accurate prediction of the characteristics and magnitude of changes in soil carbon emissions in temperate areas under global change scenarios. Here, an in situ field experiment was conducted in a typical grassland in Inner Mongolia in China to explore the characteristics of Rs under three different snow cover treatments, i.e., increasing snow (IS), decreasing snow (DS), and ambient snow that was regarded as the control check treatment (CK). The results showed that the range of Rs flux and cumulative emission flux in all treatments in the non-growing season in the study area ranged from 5.87 ± 0.20 to 55.11 ± 6.42 mg CO2 m−2 h−1 and from 22.81 ± 0.68 to 26.36 ± 0.41 g C m−2, respectively. During the observation period, the depth of the largest snow cover for each treatment did not exceed 18 cm, and none of the snow treatments caused significant variations in Rs flux (p > 0.05). However, the cumulative flux of Rs in the whole non-growing season was only stimulated significantly by 15.6% by the IS treatment compared with that of CK. The relatively high Rs flux in the non-growing season was observed to mainly occur in the soil deeply frozen period (DFP) and the soil melting period (SMP). Further analysis revealed that Rs flux under different snow treatments were mainly positively correlated with soil temperature during SMP. The main factors controlling Rs varied with different sampling periods. Our findings suggest that the non-growing season is also an important period of non-negligible carbon emissions from typical grassland soils in temperate zones.
Non-12α-Hydroxylated Bile Acids Improve Piglet Growth Performance by Improving Intestinal Flora, Promoting Intestinal Development and Bile Acid Synthesis
Authors: Qin Jianwei , Wei Xinke , Cao Mingming , Shi Baoming
Published: October 2023

As an emulsifier and bioactive substance, bile acids (BAs) participate in the absorption of nutrients and in various physiological processes. The objective of this experiment was to investigate the effects of non-12α-hydroxylated BAs (including hyocholic acid, hyodeoxycholic acid and chenodeoxycholic acid, from now on referred to as NBAs) on growth performance, BAs metabolism and the intestinal flora of piglets. The experiment included four groups, with eight piglets per group. The four groups of pigs were fed 0, 60, 120 and 180 mg/kg of NBAs, respectively. The results show that adding NBAs significantly increased the final weight (FW), average daily feed intake (ADFI), average daily gain (ADG), and digestibility of crude fat (EE) and organic matter (OM) in piglets (p < 0.05). Adding NBAs significantly increased the villus height (VH) of the jejunum and ileum (p < 0.05). In addition, NBAs supplementation increased the content of urea nitrogen (BUN) and creatinine (CREA) as well as the ratio of urea nitrogen to creatinine (BUN/CREA) in serum (p < 0.05). Adding NBAs can affect the genes related to BAs enterohepatic circulation. Specifically, adding NBAs significantly decreased the relative mRNA abundance of FXR in the liver (p < 0.05), significantly increased the relative mRNA abundance of CYP27A1 (p < 0.05), and significantly increased the relative mRNA abundance of NTCP (p < 0.05). Adding NBAs also significantly decreased the relative mRNA abundance of FXR in the ileum (p < 0.05). In the full-length 16S rDNA sequencing analysis, ten biomarkers were found from the gate to the species level. NBAs mainly enriched Lactobacillus_Johnsonii and decreased the abundance of Streptococcus_alactolyticus. Short-chain fatty acids (SCFAs) content in the colon was significantly increased (p < 0.05). These results indicate that NBAs supplementation can improve the growth performance of piglets, promote the development of the bile acid replacement pathway and improve intestinal flora.
Evaluating the Potency of Selected Antibiotic Medications Dispensed in Community Pharmacies in Gwale, Kano, Nigeria
Authors: Mukhtar Muhammad Dauda , Rufa’i Fatihu Ahmad , Yola Abdurrahaman Umar , Babba Nafisa Ibrahim , Baecker Daniel
Published: October 2023

The worsening of antibiotic resistance is a multifactorial process. One aspect of this is the counterfeiting of antibiotic medications. This is supposed to be particularly high in developing countries, including Nigeria. Therefore, the potency of some antibiotic drugs dispensed in community pharmacies in Gwale, Kano, Nigeria, was investigated in this case study. Three products, each from different manufacturers, with the active ingredients of ceftriaxone, gentamicin, ciprofloxacin, and metronidazole, respectively, were included in this study. By means of a disc-diffusion assay, the effect against the typed strains Staphylococcus aureus (ATCC 25923) and Escherichia coli (ATCC 25922) as well as Clostridium tetani isolated from soil was tested. Clinical isolates of S. aureus and E. coli were also used. While antibiotics, with the exception of ciprofloxacin-containing preparations against C. tetani, showed acceptable efficacy against the typed strains by comparison with the clinical science laboratory references, a predominant failure was observed with the clinical isolates. Thus, the investigated drug preparations can be considered of acceptable quality for the treatment of susceptible bacterial infections. This excludes counterfeits in the sampled preparations. However, the insufficient efficacy against clinical isolates further documents the severity of nosocomial bacteria.
Average Glandular Dose (AGD) and Radiation Dose Optimization in Screen-Film and Digital X-ray Mammography
Authors: Suliman Ibrahim I. , Mohamed Sawsan , Mahadi Alaa , Bashier Einas , Farah Atifa , Hassan Nada , Ibrahem Moez A. , Mohammed Ali Mohammed Khalil , Ahmed Nada A.
Published: October 2023

We determined the average glandular dose (AGD) from the craniocaudally (CC) and mediolateral oblique (MLO) views of 496 breasts (247 women) at eight clinics in Sudan. The incident air kerma from the X-ray tube output values and the typical patient-specific breast exposure factors were measured. The AGD values were inferred from the measured incident air kerma and breast-specific dose-conversion coefficients. The AGD per CC and MLO projection and per woman ranged from 0.56 to 2.89 mGy (average: 1.36), 0.48 to 2.08 mGy (average: 1.19), and 2.08 to 9.94 mGy (average: 5.10). The proposed national diagnostic reference levels (mGy) for digital mammography are 1.8 and 1.6 mGy for CC and MLO projection, respectively. Establishing the proposed diagnostic reference levels is an essential step in ensuring patient protection from radiation and will help promote dose optimization for X-ray mammography at the national level and beyond. These results provide important baseline data that can be used to formulate national diagnostic reference levels.
Nectarine Disease Identification Based on Color Features and Label Sparse Dictionary Learning with Hyperspectral Images
Authors: Miao Ronghui , Wu Jinlong , Yang Hua , Huang Fenghua
Published: October 2023

Fruit cracking and rust spots are common diseases of nectarines that seriously affect their yield and quality. Therefore, it is essential to construct fast and accurate disease-identification models for agricultural products. In this paper, a sparse dictionary learning method was proposed to realize the rapid and nondestructive identification of nectarine disease based on multiple color features combined with improved LK-SVD (Label K-Singular Value Decomposition). According to the color characteristics of the nectarine itself and the significant color differences existing in the three categories of nectarine (diseased, normal, and background parts), multiple color spaces of RGB, HSV, Lab, and YCbCr were studied. It was concluded that the G channel in RGB space, Y channel in YCbCr space, and L channel in Lab space can better distinguish the diseased part from the other parts. At the model-training stage, pixels of the diseased, normal, and background parts in the nectarine image were randomly selected as the initial training sets, and then, the neighboring image blocks of the pixels were selected to construct the feature vectors based on the above color space channels. An improved LK-SVD dictionary learning algorithm was proposed that integrated the category label into the process of dictionary learning, and thus, an over-complete feature dictionary with significant discrimination was obtained. At the model-testing stage, the orthogonal matching pursuit (OMP) algorithm was used for sparse reconstruction of the original data, which can obtain the classification categories based on the optimized feature dictionary. The experimental results show that the sparse dictionary learning method based on multi-color features combined with improved LK-SVD can identify fruit cracking and rust spot diseases of nectarines quickly and accurately, and the average overall classification accuracies were 92.06% and 88.98%, respectively, which were better than those of k-nearest neighbor (KNN), support vector machine (SVM), DeepLabV3+, and Unet++; the identification results of DeepLabV3+ and Unet++ were also relatively high, but their average time costs were much higher, requiring 126.46~265.65 s. It is demonstrated that this study can provide technical support for disease identification in agricultural products.
Physicochemical, Nutritional, Antioxidant, and Sensory Properties of Crackers Supplemented with Edible Insects
Authors: Ivanišová Eva , Rajnoha Marek , Harangozo Ľuboš , Kunecová Daniela , Čech Matej , Gabríny Lucia , Gálik Branislav , Banach Joanna Katarzyna , Kowalczewski Przemysław Łukasz , Pietrzak-Fiećko Renata
Published: October 2023

This study aimed to determine the physicochemical, nutritional, antioxidant, and sensory properties of crackers with the addition of edible insects. The analyses covered the contents of total dry matter, crude protein, fat and ash; amino acid composition and antioxidant activity (DPPH method); total polyphenol content; mineral compound composition (AAS); caloric value; and sensory profile (aroma, taste, general appearance, overall acceptability) of the durable pastry—crackers supplemented with 5% of powder of edible insects (cricket, mealworm, and grasshopper). Appropriate research methods were used for analyses. Studies have shown that the contents of dry matter, crude protein, fat and all amino acids detected were higher in the samples with the addition of insects compared to the control sample. The highest antioxidant activity was assayed in the sample with the addition of grasshopper powder. The highest content of total polyphenols was determined in the sample with the addition of mealworm. The addition of insects positively affected the content of mineral substances—especially zinc—in the sample with cricket powder addition. The results of this study show that edible insects are an attractive raw material, which can in future expand the assortment of food products available on the market and increase the nutritional benefits of enriched products.
Enabling Extremely Energy-Efficient End-to-End Secure Communications for Smart Metering Internet of Things Applications Using Static Context Header Compression
Authors: Dumay Marion , Al Haj Hassan Hussein , Surbayrole Philippe , Artis Thibaut , Barthel Dominique , Pelov Alexander
Published: October 2023

Smart metering IoT applications are among the most energy-critical in the current panorama. Metering sensors are battery-powered and are expected to have a lifetime exceeding ten years. In order to achieve such long operation duration, a generic header compression mechanism named Static Context Header Compression (SCHC) has been introduced and accepted as a standard by the Internet Engineering Task Force (IETF). This paper aims to demonstrate the energy savings enabled by the use of SCHC on a cellular IoT network by the means of real-life implementation and measurements. Experiments are conducted in a controlled environment for different scenarios and considering multiple parameters such as message size and radio conditions. Measurements show the high impact of this header compression mechanism, particularly when the radio conditions are bad and repetitions are used to improve the reliability of the transmission: a reduction of up to 40% in energy consumption is observed. Using SCHC over the non-IP transport mode (NIDD) of NB-IoT compared to the legacy IP mode also enables significant energy savings and allows the latency to be reduced while maintaining the interoperability provided by the IP layer.