bluuebunny/crossref_metadata_2025 huggingface dataset:
Native Yeasts and Lactic Acid Bacteria Isolated from Spontaneous Fermentation of Seven Grape Cultivars from the Maule Region (Chile)
Authors: Franco Wendy , Benavides Sergio , Valencia Pedro , Ramírez Cristian , Urtubia Alejandra
Published: July 2021

Grapes are a source of native yeasts and lactic acid bacteria (LAB); however, the microbial make up is dependent on the grape cultivar and the regional growth conditions. Therefore, the aim of this study was to characterize the yeast and LAB in seven grape cultivars cultivated in Chile. Grape juices were fermented at 25 °C for 7 days. Samples were collected to analyze sugar, organic acids, and ethanol. Microbial evolution was measured with culture-dependent and molecular approaches. Then, a native isolated Candida oleophila was selected for further sequential fermentations with Saccharomyces cerevisiae. The grape cultivars in the Maule showed a diversity of non-Saccharomyces yeasts, with a greater diversity observed at the beginning of the fermentation. However, species from the Hansenasporia, Metschnikowia, Torulaspora, Lachancea, and Candida genera were detected after 7 days, suggesting tolerance to environments rich in ethanol, capability may be associated to the terroir studied, which is characterized by torrid weather and antique and traditional vineyards. The alcoholic fermentation negatively impacted the LAB population, and after 7 days only Leuconostoc mesenteroides was isolated. In the sequential fermentations, C. oleophila was able to produce fermented grape juices with <1.5 g/L glucose, 12.5% (v/v) alcohol, and low concentrations of malic (<1.00 g/L) and succinic (2.05 g/L) acids, while acetic acid reached values >0.3 (g/L). To our knowledge this is the first time C. oleophila has been reported as a potential starter culture for wine production. However, more studies are necessary to fully characterize the potential of C. oleophila on wine attributes.
Stress Condition on a Restricted Sodium Diet Using Umami Substance (L-Glutamate) in a Pilot Randomized Cross-Over Study
Authors: Iwamoto Tamami , Wakita Andrea , Shikanai Saiko , Matsumoto Hideki , Hirota Mariko , Uneyama Hisayuki , Hien Vu Thi Thu , Yamamoto Shigeru
Published: July 2021

Hypertensive patients who adopt a sodium-restricted diet have difficulty maintaining this change, and this could increase stress. On the other hand, soup rich in umami substances (dashi) was reported to reduce indexes of anxiety and stress. The objective of this study was to measure mood and physiological stress indexes during administration of a sodium-restricted diet with and without an umami substance (free L-glutamate) by a cross-over randomized, single-blind, placebo-controlled trial in Japanese female university students. The baseline was measured for 5 days followed by a sodium-restricted diet intervention phase that lasted for 10 days. The Profile of Mood States questionnaire was administered, a stress marker in saliva (chromogranin-A) was measured, and the amount of sodium intake was confirmed from 24 h urine collection samples. Results showed that the sodium reduction was verified by 24 h urine excretion. The percentage of change in the stress marker from the baseline showed that the stress level in group without the umami substance was significantly higher than that in the group with the umami substance (p = 0.013) after receiving a sodium-reduced diet for 6 or more days, indicating that stress was alleviated. This study suggested that umami substances might help to ameliorate stress during a sodium-reduced diet, especially in the initial phase.
Medical Faculty’s and Students’ Perceptions toward Pediatric Electronic OSCE during the COVID-19 Pandemic in Saudi Arabia
Authors: Shaiba Lana A. , Alnamnakani Mahdi A. , Temsah Mohamad-Hani , Alamro Nurah , Alsohime Fahad , Alrabiaah Abdulkarim , Alanazi Shahad N. , Alhasan Khalid , Alherbish Adi , Mobaireek Khalid F. , Bashiri Fahad A. , AlRuthia Yazed
Published: July 2021

Background: The educational process in different medical schools has been negatively affected by the COVID-19 pandemic worldwide. As a part of the Saudi government’s attempts to contain the spread of the virus, schools’ and universities’ educational activities and face-to-face lectures have been modified to virtual classrooms. The purpose of this study was to explore the perceptions of the faculty and the students of an electronic objective structured clinical examination (E-OSCE) activity that took place during the COVID-19 pandemic in the oldest medical school in Saudi Arabia. Methods: An e-OSCE style examination was designed for the final-year medical students by the pediatrics department, College of Medicine at King Saud University in Riyadh, Saudi Arabia. The examination was administered by Zoom™ video conferencing where both students and faculty participated through their laptop or desktop computers. In order to explore the students’ and the faculty’s perceptions about this experience, a newly designed 13-item online questionnaire was administered at the end of the e-OSCE. Results: Out of 136 participants (23 faculty and 112 students), 73 respondents (e.g., 54% response rate) filled out the questionnaire. Most of the respondents (69.8%) were very comfortable with this new virtual experience. Most participants (53.4%) preferred the e-OSCE compared to the classic face-to-face clinical OSCE during the pandemic. Regarding the e-OSCE assessment student tool, 46.6% reported that it is similar to the classic face-to-face OSCE; however, 38.4% felt it was worse. Conclusions: The e-OSCE can be a very effective alternative to the classic face-to-face OSCE due to the current circumstances that still pose a significant risk of infection transmission. Future studies should examine different virtual strategies to ensure effective OSCE delivery from the perspective of both faculty and students.
Lapnurse—A Blended Learning Course for Nursing Education in Minimally Invasive Surgery: Design and Experts’ Preliminary Validation of Its Online Theoretical Module
Authors: Ortega-Morán Juan Francisco , Pagador Blas , Maestre-Antequera Juan , Sánchez-Fernández Javier , Arco Antonio , Monteiro Francisco , Sánchez-Margallo Francisco M.
Published: July 2021

Background: The implantation of Minimally Invasive Surgery (MIS) leads to the specialization of nurses in this surgical field. However, there is no standard curriculum of MIS Nursing in Europe. Spanish and Portuguese nurses are inexperienced and have poor training in MIS. For that, a blended learning course for nursing education in MIS (Lapnurse) has been developed. This work aims to detail the course design and to preliminary validate by experts its online theoretical module. Methods: Lapnurse consists of an online module with nine theoretical lessons and a face-to-face module with three practical lessons. The e-learning environment created to provide the online module, with didactic contents based on surgical videos and innovative 3D designs, has been validated by two technicians (functionality) and four nurses with teaching experience in MIS (usability and content). Results: The E-learning platform meets all technical requirements, provides whole and updated multimedia contents correctly applied for educational purposes, incorporates interactivity with 3D designs, and has an attractive, easy-to-use and intuitive design. Conclusions: The lack of knowledge in MIS of Spanish and Portuguese nurses could be addressed by the blended learning course created, Lapnurse, where the e-learning environment that provides theoretical training has obtained a positive validation.
Shooting Your Accuracy in the Foot? Examining the Short-Term Effect of Playing an Action or Strategy Video Game on Cognitive Control
Authors: Rice Michaela , Lease Alexis , Walker MaLia , Bailey Kira
Published: July 2021

The current study examined the effects of brief video game exposure on cognitive control using event-related potentials (ERPs). Cognitive control was assessed by ERP components associated with the ability to detect (N2) and resolve (SP) conflict when the conflict was either expected or unexpected. After playing either an action or strategy video game, participants completed a counting Stroop task while ERPs were recorded. The proportion of congruent to incongruent trials was manipulated across blocks to create conditions where conflict was expected or unexpected. While visual inspection of the behavioral and neural data revealed interesting patterns by video game, none of those effects were statistically significant. This is consistent with some previous work and inconsistent with other published data, suggesting that there is still much to learn about the relationship between cognitive control and video game experience.
Improvement in 6-Minute Walking Distance after Supervised Exercise Training Is Related to Changes in Quality of Life in Patients with Lower Extremity Peripheral Artery Disease
Authors: Lanzi Stefano , Calanca Luca , Berchtold André , Mazzolai Lucia
Published: July 2021

This study aimed to investigate the relationship between supervised exercise training (SET)-induced changes in treadmill performance and 6 min walking distance, and changes in general (physical and mental) self-perceived health-related quality of life (HRQoL) in symptomatic patients with lower extremity peripheral artery disease (PAD). This is an observational study investigating Fontaine stage II PAD patients participating in 3-month SET. Before and following SET, treadmill performance (pain-free (PFWD) and maximal (MWD)), and 6 min walking distance (6MWD) were assessed. Self-perceived HRQoL was assessed with the Medical Outcomes Study Short-Form 36 (SF-36). Ankle- and toe-brachial indexes were also measured. One-hundred forty-seven patients with PAD were included (64.9 ± 9.6 y, 70% men). After SET, PFWD (+102%, p ≤ 0.001), MWD (+87%, p ≤ 0.001), and 6MWD (+14%, p ≤ 0.001) significantly increased. All eight SF-36 subscale scores significantly improved following SET (p ≤ 0.04). SET significantly improved physical and mental component summaries of the SF-36 (p ≤ 0.001). Larger increases in 6MWD were associated with greater improvements in physical (β = 0.19; p = 0.02) and mental (β = 0.24; p = 0.005) component summaries of the SF-36. No significant relationship was observed between changes in treadmill performance and changes in physical and mental component summaries of the SF-36. These results show that improvements in 6MWD following SET are related to improvements in general self-perceived HRQoL in patients with symptomatic lower extremity PAD. On the contrary, changes in treadmill performance were not related to improvements in HRQoL. These results suggest that the 6 min walking test is an essential outcome measure to assess overall patient functional status following interventions in patients with PAD.
Outcomes and Timing of Bedside Percutaneous Tracheostomy of COVID-19 Patients over a Year in the Intensive Care Unit
Authors: Tetaj Nardi , Maritti Micaela , Stazi Giulia , Marini Maria , Centanni Daniele , Garotto Gabriele , Caravella Ilaria , Dantimi Cristina , Fusetti Matteo , Santagata Carmen , Macchione Manuela , De Angelis Giada , Giansante Filippo , Busso Donatella , Di Lorenzo Rachele , Scarcia Silvana , Carucci Alessandro , Cabas Ricardo , Gaviano Ilaria , Petrosillo Nicola , Antinori Andrea , Palmieri Fabrizio , D’Offizi Gianpiero , Ianniello Stefania , Campioni Paolo , Pugliese Francesco , Vaia Francesco , Nicastri Emanuele , Ippolito Giuseppe , Marchioni Luisa ,   ICU COVID-19 Study Group
Published: July 2021

Background: The benefits and timing of percutaneous dilatational tracheostomy (PDT) in Intensive Care Unit (ICU) COVID-19 patients are still controversial. PDT is considered a high-risk procedure for the transmission of SARS-CoV-2 to healthcare workers (HCWs). The present study analyzed the optimal timing of PDT, the clinical outcomes of patients undergoing PDT, and the safety of HCWs performing PDT. Methods: Of the 133 COVID-19 patients who underwent PDT in our ICU from 1 April 2020 to 31 March 2021, 13 patients were excluded, and 120 patients were enrolled. A trained medical team was dedicated to the PDT procedure. Demographic, clinical history, and outcome data were collected. Patients who underwent PDT were stratified into two groups: an early group (PDT ≤ 12 days after orotracheal intubation (OTI) and a late group (>12 days after OTI). An HCW surveillance program was also performed. Results: The early group included 61 patients and the late group included 59 patients. The early group patients had a shorter ICU length of stay and fewer days of mechanical ventilation than the late group (p < 0.001). On day 7 after tracheostomy, early group patients required fewer intravenous anesthetic drugs and experienced an improvement of the ventilation parameters PaO2/FiO2 ratio, PEEP, and FiO2 (p < 0.001). No difference in the case fatality ratio between the two groups was observed. No SARS-CoV-2 infections were reported in the HCWs performing the PDTs. Conclusions: PDT was safe and effective for COVID-19 patients since it improved respiratory support parameters, reduced ICU length of stay and duration of mechanical ventilation, and optimized the weaning process. The procedure was safe for all HCWs involved in the dedicated medical team. The development of standardized early PDT protocols should be implemented, and PDT could be considered a first-line approach in ICU COVID-19 patients requiring prolonged mechanical ventilation.
Effect of Significant Coronary Artery Stenosis on Prognosis in Patients with Vasospastic Angina: A Propensity Score-Matched Analysis
Authors: Kim Hyun-Jin , Lee Min-Ho , Jo Sang-Ho , Seo Won-Woo , Kim Hack-Lyoung , Lee Kwan-Yong , Yang Tae-Hyun , Her Sung-Ho , Han Seung-Hwan , Lee Byoung-Kwon , Park Keun-Ho , Rha Seung-Woon , Gwon Hyeon-Cheol , Choi Dong-Ju , Baek Sang-Hong
Published: July 2021

Vasospastic angina (VA) is a functional disease of the coronary artery and occurs in an angiographically normal coronary artery. However, it may also occur with coronary artery stenosis. We investigated the effect of coronary artery stenosis on clinical outcomes in VA patients. Study data were obtained from a prospective multicenter registry that included patients who had symptoms of VA. Patients were classified into two groups according to presence of significant coronary artery stenosis. Among 1920 patients with VA, 189 patients were classified in the “significant stenosis” group. The one-year composite clinical events rate was significantly higher in the significant stenosis group than in the “no significant stenosis” group (5.8% vs. 1.4%, respectively; p < 0.001). Additionally, the prevalence of ACS was significantly greater in the “significant stenosis” group (4.8% vs. 0.9%, respectively; p < 0.001). After propensity score matching, the adverse effects of significant stenosis remained. In addition, significant stenosis was independently associated with a 6.67-fold increased risk of ACS in VA patients. In conclusion, significant coronary artery stenosis can increase the adverse clinical outcomes in VA patients at long-term follow-up. Clinicians should manage traditional risk factors associated with atherosclerosis and control vasospasm as well as reduce the burden of atherosclerosis.
Nonstationary Extreme Value Analysis of Nearshore Sea-State Parameters under the Effects of Climate Change: Application to the Greek Coastal Zone and Port Structures
Authors: Galiatsatou Panagiota , Makris Christos , Krestenitis Yannis , Prinos Panagiotis
Published: July 2021

In the present work, a methodological framework, based on nonstationary extreme value analysis of nearshore sea-state parameters, is proposed for the identification of climate change impacts on coastal zone and port defense structures. The applications refer to the estimation of coastal hazards on characteristic Mediterranean microtidal littoral zones and the calculation of failure probabilities of typical rubble mound breakwaters in Greek ports. The proposed methodology hinges on the extraction of extreme wave characteristics and sea levels due to storm events affecting the coast, a nonstationary extreme value analysis of sea-state parameters and coastal responses using moving time windows, a fitting of parametric trends to nonstationary parameter estimates of the extreme value models, and an assessment of nonstationary failure probabilities on engineered port protection. The analysis includes estimation of extreme total water level (TWL) on several Greek coasts to approximate the projected coastal flooding hazard under climate change conditions in the 21st century. The TWL calculation considers the wave characteristics, sea level height due to storm surges, mean sea level (MSL) rise, and astronomical tidal ranges of the study areas. Moreover, the failure probabilities of a typical coastal defense structure are assessed for several failure mechanisms, considering variations in MSL, extreme wave climates, and storm surges in the vicinity of ports, within the framework of reliability analysis based on the nonstationary generalized extreme value (GEV) distribution. The methodology supports the investigation of future safety levels and possible periods of increased vulnerability of the studied structure to different ultimate limit states under extreme marine weather conditions associated with climate change, aiming at the development of appropriate upgrading solutions. The analysis suggests that the assumption of stationarity might underestimate the total failure probability of coastal structures under future extreme marine conditions.
Ether Oxidation by an Evolved Fungal Heme-Peroxygenase: Insights into Substrate Recognition and Reactivity
Authors: Mireles Raul , Ramirez-Ramirez Joaquin , Alcalde Miguel , Ayala Marcela
Published: July 2021

Ethers can be found in the environment as structural, active or even pollutant molecules, although their degradation is not efficient under environmental conditions. Fungal unspecific heme-peroxygenases (UPO were reported to degrade low-molecular-weight ethers through an H2O2-dependent oxidative cleavage mechanism. Here, we report the oxidation of a series of structurally related aromatic ethers, catalyzed by a laboratory-evolved UPO (PaDa-I) aimed at elucidating the factors influencing this unusual biochemical reaction. Although some of the studied ethers were substrates of the enzyme, they were not efficiently transformed and, as a consequence, secondary reactions (such as the dismutation of H2O2 through catalase-like activity and suicide enzyme inactivation) became significant, affecting the oxidation efficiency. The set of reactions that compete during UPO-catalyzed ether oxidation were identified and quantified, in order to find favorable conditions that promote ether oxidation over the secondary reactions.
The Effect of Amanita rubescens Pers Developmental Stages on Aroma Profile
Authors: Štefániková Jana , Martišová Patrícia , Šnirc Marek , Kunca Vladimír , Árvay Július
Published: July 2021

The dichloromethane extraction was applied to extracted volatile compounds of the six developmental stages of caps and stipes of an Amanita rubescens mushroom and the relative contents were measured with the gas chromatography-mass spectrometry. The number of identified compounds ranged between 53 and 52, respectively, with a high ratio of alkane volatiles. The significant differences between the aroma compounds were determined in caps to identify their stages of development. The fully mature stage caps were characterized by 4,6-dimethyl-dodecane (7.69 ± 1.15%), 2-hexyl-1-decanol (11.8 ± 1.61%), 1,3-di-tert-butylbenzene (11.4 ± 1.25%), heptadecyl pentadecafluorooctanoate (2.16 ± 0.31%), and 2-hexyl-1-dodecanol (13.5 ± 1.33%). Niacinamide (3.90 ± 0.07%) and glycerol (3.62 ± 1.27%) was present in the caps in the early-stage of the rotting mushroom, which represented the 10th–12th day of fructification. The caps and stipes from the 12th–15th day of fructification were characterized by 2,3-butanediol (11.7 ± 0.13% and 8.00 ± 0.10%, respectively). Moreover, the caps from this developmental stage were characterized by 2-methyl- and 3-methyl butanoic acids (0.18 ± 0.03% and 0.33 ± 0.02%, respectively) which are typical for the rotting stage. In this study, we confirmed the effect of A. rubescens developmental stages on the aroma profile.
How to Evaluate the Green Utilization Efficiency of Cultivated Land in a Farming Household? A Case Study of Shandong Province, China
Authors: Qu Yi , Lyu Xiao , Peng Wenlong , Xin Zongfei
Published: July 2021

The behavior of farming households is the most direct factor involved in the transition of cultivated land utilization from high-input/high-output to green and sustainable utilization mode. Improving farming households’ green utilization efficiency of cultivated land (GUECL) is of great significance in facilitating agricultural green development in China. However, there are few studies on GUECL based on the micro-perspective of farming households that cover the comprehensive benefits to the economy, ecology, and society. This paper builds a theoretical analysis framework of farming households’ green utilization of cultivated land and uses the super-efficiency EBM model and a questionnaire to conduct an empirical analysis of 952 farming households in Shandong Province to evaluate the green utilization efficiency of cultivated land. The results show that the GUECL of the farming households is generally not high, with an average value of 0.67, and can be further improved. The higher the GUECL, the lower the input and undesired output per unit yield and per unit output value. Tobit regression results show that a farming household’s per capita income is significantly positively correlated with the GUECL, while agricultural insurance, agricultural subsidies, cultivated land scale, cultivated land fragmentation, and regional economic level are significantly negatively correlated with the GUECL. In addition, recommendations can be made on promoting and innovating agricultural green development technology, popularizing and publicizing farming households’ thoughts on the green utilization of cultivated land, and ensuring and improving rural green life so as to provide a reference for promoting green transition of cultivated land utilization with diversified coordination and multiple measures.
Promoting Literacy in Adult Second Language Learners: A Systematic Review of Effective Practices
Authors: Piccinin Sabrina , Dal Maso Serena
Published: July 2021

Literacy is an essential tool for functioning in a modern society and, as such, it is often taken for granted when developing second language learning curricula for people who need to learn another language. However, almost 750 million people around the world cannot read and write, because of limited or absent formal education. Among them, migrants face the additional challenge of having to learn a second language as they settle in a new country. Second language research has only recently started focusing on this population, whose needs have long been neglected. This contribution presents a systematic review of the classroom-based research conducted with such learners and aims at identifying the teaching practices that have proven to be successful and the principles that should inform curriculum design when working with this population. A first observation emerging from the review concerns the scarcity of experimentally validated studies within this domain. Nonetheless, based on the results of the available literature, this work highlights the importance of contextualized phonics teaching and of oral skills development, which turn out to be most effective when emphasis is put on learners’ cultural identities and native languages.
Identification of Putative Biosynthetic Gene Clusters for Tolyporphins in Multiple Filamentous Cyanobacteria
Authors: Jin Xiaohe , Zhang Yunlong , Zhang Ran , Nguyen Kathy-Uyen , Lindsey Jonathan S. , Miller Eric S.
Published: July 2021

Tolyporphins A–R are unusual tetrapyrrole macrocycles produced by the non-axenic filamentous cyanobacterium HT-58-2. A putative biosynthetic gene cluster for biosynthesis of tolyporphins (here termed BGC-1) was previously identified in the genome of HT-58-2. Here, homology searching of BGC-1 in HT-58-2 led to identification of similar BGCs in seven other filamentous cyanobacteria, including strains Nostoc sp. 106C, Nostoc sp. RF31YmG, Nostoc sp. FACHB-892, Brasilonema octagenarum UFV-OR1, Brasilonema octagenarum UFV-E1, Brasilonema sennae CENA114 and Oculatella sp. LEGE 06141, suggesting their potential for tolyporphins production. A similar gene cluster (BGC-2) also was identified unexpectedly in HT-58-2. Tolyporphins BGCs were not identified in unicellular cyanobacteria. Phylogenetic analysis based on 16S rRNA and a common component of the BGCs, TolD, points to a close evolutionary history between each strain and their respective tolyporphins BGC. Though identified with putative tolyporphins BGCs, examination of pigments extracted from three cyanobacteria has not revealed the presence of tolyporphins. Overall, the identification of BGCs and potential producers of tolyporphins presents a collection of candidate cyanobacteria for genetic and biochemical analysis pertaining to these unusual tetrapyrrole macrocycles.
Recombinase-Aided Amplification Coupled with Lateral Flow Dipstick for Efficient and Accurate Detection of Porcine Parvovirus
Authors: He Yihong , Chen Wenxian , Fan Jindai , Fan Shuangqi , Ding Hongxing , Chen Jinding , Yi Lin
Published: July 2021

Porcine parvovirus (PPV) infection is the primary cause of SMEDI (stillbirth; mummification; embryonic death; infertility) syndrome, which is a global burden for the swine industry. Thus, it is crucial to establish a rapid and efficient detection method against PPV infection. In the present work, we developed a recombinase-aided amplification (RAA) assay, coupled with a lateral flow dipstick (LFD), to achieve an amplification of PPV DNA at 37 °C within 15 min. The detection limits of PPV RAA-LFD assay were 102 copies/μL recombinant plasmid pMD19-T-VP1, 6.38 × 10−7 ng/μL PPV DNA, and 10−1 TCID50/mL virus, respectively. This method was highly specific for PPV detection with no cross-reactivity for other swine pathogens. In contrast to polymerase chain reaction (PCR), the PPV RAA-LFD assay is more sensitive and cost-saving. Hence, the established PPV RAA-LFD assay provided an alternative for PPV detection, especially in resource-limited regions.
Emergency Quantity Discount Contract with Suppliers Risk Aversion under Stochastic Price
Authors: Wu Shuangsheng , Li Qi
Published: July 2021

This paper constructs an emergency quantity discount contract to explore the inherent law of the contract coordinating the supply chain with stochastic market demand and price and the risk-averse supplier. Meanwhile, the conditional value-at-risk (CVaR) risk measure criterion is revised to study the influence of supplier’s risk aversion attitude on supply chain coordination. The results show that supplier risk aversion will cause the bifurcation of the relevant factors in the supply chain under the stochastic price. Within the bifurcation region, the supply chain cannot be coordinated; out of the bifurcation region, the supply chain can achieve coordination. The supply chain related factors’ variation range in the bifurcation region is related to the step size of the risk aversion factor and the normal distribution function’s variance of the market demand, and it increases with the latter.
Family SES Is Associated with the Gut Microbiome in Infants and Children
Authors: Lewis Candace R. , Bonham Kevin S. , McCann Shelley Hoeft , Volpe Alexandra R. , D’Sa Viren , Naymik Marcus , De Both Matt D. , Huentelman Matthew J. , Lemery-Chalfant Kathryn , Highlander Sarah K. , Deoni Sean C. L. , Klepac-Ceraj Vanja
Published: July 2021

Background: While early life exposures such as mode of birth, breastfeeding, and antibiotic use are established regulators of microbiome composition in early childhood, recent research suggests that the social environment may also exert influence. Two recent studies in adults demonstrated associations between socioeconomic factors and microbiome composition. This study expands on this prior work by examining the association between family socioeconomic status (SES) and host genetics with microbiome composition in infants and children. Methods: Family SES was used to predict a latent variable representing six genera abundances generated from whole-genome shotgun sequencing. A polygenic score derived from a microbiome genome-wide association study was included to control for potential genetic associations. Associations between family SES and microbiome diversity were assessed. Results: Anaerostipes, Bacteroides, Eubacterium, Faecalibacterium, and Lachnospiraceae spp. significantly loaded onto a latent factor, which was significantly predicted by SES (p < 0.05) but not the polygenic score (p > 0.05). Our results indicate that SES did not predict alpha diversity but did predict beta diversity (p < 0.001). Conclusions: Our results demonstrate that modifiable environmental factors influence gut microbiome composition at an early age. These results are important as our understanding of gut microbiome influences on health continue to expand.
Geographic Area of Collection Determines the Chemical Composition and Antimicrobial Potential of Three Extracts of Chilean Propolis
Authors: Alvear Marysol , Santos Estela , Cabezas Felipe , Pérez-SanMartín Andrés , Lespinasse Mónica , Veloz Jorge
Published: July 2021

The biological properties of chilean propolis have been described and include antibacterial, antifungal and antibiofilm activities. Propolis has a strong antimicrobial potential. Clinical experiences with synthetic antibiotics indicated the need to discover new sources of bioactive compounds associated with ethnopharmacological knowledge or natural sources such as propolis. The microscopic analysis of pollen grains from plants allows us to determine the botanical origin of the propolis samples. In Angol, sample pollen grains were obtained from fodder plants (Sorghum bicolor; Lotus sp.) and trees, such as Acacia sp., Pinus radiata, Eucalyptus sp. and Salix babylonica. Propolis from the Maule region contains pollen grains from endemic plants such as Quillaja saponaria. Finally, the sample obtained from Melipilla presented a wider variety of pollen extracted from vegetable species.Colorimetric assays performed to quantify the total polyphenols present in Chilean propolis samples established that PCP2 (Angol sample) showed high amounts of phenolics compounds, with significant statistical differences in comparison with the other samples. The main compounds identified were pinocembrin, quercetin and caffeic acid phenethyl ester (CAPE). The Angol sample showed a high content of polyphenols.Studies that determine the influence of geographical and floral variables on the chemical composition of propolis are a valuable source of information for the study of its biological properties.
Anti-Inflammatory Activity of a Medicinal Herb Extract Mixture, HM-V, on an Animal Model of DNCB-Induced Chronic Skin Inflammation
Authors: Park Sungbae , Lee Sangmin , Weon Youngho , Kim Taewook , Kim Hakwon , Lee Taehoon
Published: July 2021

Chronic inflammatory skin diseases, such as atopic dermatitis, are caused by the accumulation of immune cells and the overproduction of chemokines, including CCL17 and CCL22, due to the activation of pro-inflammatory cytokines secreted from keratinocytes. In the present study, the inhibitory activity of HM-V on tumor necrosis factor alpha (TNF-α)/interferon gamma (IFN-γ)-induced pro-inflammatory cytokines was examined in human keratinocytes (HaCaTs) and 2,4-dinitrofluorobenzene (DNCB)-induced chronic skin contact dermatitis animal models. Traditional Asian medicinal herb extracts mixture (HM-V), which have been extensively used in Asian medicine, were utilized. In TNF-α/IFN-γ-induced HaCaTs, HM-V strongly inhibited mRNA and protein expression of CCL17 and CCL22 in a concentration-dependent manner. The expression of pro-inflammatory cytokines such as TNF-α, IL-1β, and IL-6 was also inhibited. Therefore, localized administration of HM-V in the DNCB-induced animal model alleviated immune cell deposition and skin inflammation. The results indicate that HM-V exerts inhibitory effects on keratinocyte production of CCL17 and CCL22. Furthermore, HM-V may be a useful anti-inflammatory agent for the prevention and treatment of inflammatory skin diseases.
Horseradish Essential Oil as a Promising Anti-Algal Product for Prevention of Phytoplankton Proliferation and Biofouling
Authors: Bácsi István , Gonda Sándor , Nemes-Kókai Zsuzsanna , B-Béres Viktória , Vasas Gábor
Published: July 2021

Increased proliferation of algae is a current problem in natural and artificial water bodies. Controlling nutrients is the most sustainable treatment of increased algal proliferation, however in certain cases, it is not sufficiently available, or it does not provide results fast enough. Chemicals derived from natural sources, which could be effective in low concentrations and are biodegradable, may have an advantage over conventional chemical treatments. The main aim of the present study was to investigate the anti-cyanobacterial and anti-algal properties of allyl-isothiocyanate-containing essential oil produced from horseradish roots with a complex approach of the topic: on laboratory strains of cyanobacteria and eukaryotic algae, on microcosms containing natural phytoplankton assemblages, and on semi-natural biofilms. The results show that acute treatment can significantly reduce the viability of all the tested cyanobacteria and eukaryotic algae. Results of microcosm experiments with natural phytoplankton assemblages show that horseradish essential oil from 7.1 × 10−6% (v/v) is applicable to push back phytoplankton proliferation even in natural assemblages. The individual number in the biofilm was dropped down to one-fifth of the original individual number, so 7.1 × 10−6% (v/v) and higher concentration of the essential oil can be considered as a successful treatment against biofouling.
Determination of 1-Deoxynojirimycin (1-DNJ) in Leaves of Italian or Italy-Adapted Cultivars of Mulberry (Morus sp.pl.) by HPLC-MS
Authors: Marchetti Lucia , Saviane Alessio , Montà Antonella dalla , Paglia Graziella , Pellati Federica , Benvenuti Stefania , Bertelli Davide , Cappellozza Silvia
Published: July 2021

Recently, 1-DNJ has been widely studied by scientists for its capacity to inhibit α-glucosidase and reduce postprandial blood glucose and fat accumulation. To the best of our knowledge, this is the first analytical determination of 1-DNJ in Morus sp.pl. leaves carried out on Italian crops, and it could be used as a reference to assess the quality of the plant material in comparison to Far Eastern Asia cultivations. The effects of two thermal treatments were compared to test the incidence of the drying process on the 1-DNJ extractability. In addition, two harvesting seasons in the same year (2017) and two subsequent harvesting years (2017–2018) were considered. The amount of 1-DNJ herein found was comparable to that reported in the scientific literature for Asian cultivations. The increase in 1-DNJ along the summer and the higher level of this compound in the apical leaves also complies with previous findings. However, a strong implication for the climatic conditions in the different years and a significant interaction between climate and genotypes suggest exploring very carefully the agronomic practices and selecting cultivars according to different environmental conditions with a view to standardize the 1-DNJ amount in leaves.
The WL_PCR: A Planning for Ground-to-Pole Transition of Wheeled-Legged Pole-Climbing Robots
Authors: Wang Yankai , Du Qiaoling , Zhang Tianhe , Xue Chengze
Published: July 2021

Hybrid mobile robots with two motion modes of a wheeled vehicle and truss structure with the ability to climb poles have significant flexibility. The motion planning of this kind of robot on a pole has been widely studied, but few studies have focused on the transition of the robot from the ground to the pole. In this study, a locomotion strategy of wheeled-legged pole-climbing robots (the WL_PCR) is proposed to solve the problem of ground-to-pole transition. By analyzing the force of static and dynamic process in the ground-to-pole transition, the condition of torque provided by the gripper and moving joint is proposed. The mathematical expression of Centre of Mass (CoM) of the wheeled-legged pole-climbing robots is utilized, and the conditions for the robot to smoothly transition from the ground to the vertical pole are proposed. Finally, the feasibility of this method is proved by the simulation and experimentation of a locomotion strategy on wheeled-legged pole-climbing robots.
Muscular Strength, Power, and Endurance Adaptations after Two Different University Fitness Classes
Authors: Hollerbach Brittany S. , Cosgrove Sarah J. , DeBlauw Justin A. , Jitnarin Nattinee , Poston Walker S. C. , Heinrich Katie M.
Published: July 2021

Physical activity (PA) classes help college students add weekly PA, which can help improve health and maintain body weight. Traditional weight training (TWT) can improve strength and aerobic capacity. High-intensity functional training such as CrossFit® (CF) provides time-efficient workouts with both muscle strengthening and aerobic exercises. Limited research has compared these classes for college students. We examined changes in muscular strength, power, and endurance as well as body composition. Participants were 85 healthy college students enrolled in TWT (n = 36, age 22.6 ± 4.1 years, 72.2% male) or CF (n = 49, age 21.8 ± 3.2 years, 55.1% male) classes meeting twice/wk for 8 weeks between October 2017 and May 2018. Baseline and posttest measurements included a vertical jump, grip strength, a 2 min push-up test, a 1 min squat test, height, weight, and a bioelectrical impedance analysis. Although no significant group × time interactions were found, there was a significant main effect of time for push-ups and squats (both p < 0.001). Participants enjoyed the classes and most planned to continue. Both classes improved muscular endurance although no significant differences were found between them. Activity classes provide college students with an option for increasing their weekly PA and help maintain body composition. Future research should examine the benefits from longer or more frequent classes.
Theoretical Three-Dimensional Zinc Complexes with Glutathione, Amino Acids and Flavonoids
Authors: Pérez de la Lastra José Manuel , Andrés-Juan Celia , Plou Francisco J. , Pérez-Lebeña Eduardo
Published: July 2021

Zinc plays an important role in the regulation of many cellular functions; it is a signaling molecule involved in the transduction of several cascades in response to intra and extracellular stimuli. Labile zinc is a small fraction of total intracellular zinc, that is loosely bound to proteins and is easily interchangeable. At the cellular level, several molecules can bind labile zinc and promote its passage across lipophilic membranes. Such molecules are known as ionophores. Several of these compounds are known in the scientific literature, but most of them can be harmful to human health and are therefore not allowed for medical use. We here performed a theoretical three-dimensional study of known zinc ionophores, together with a computational energetic study and propose that some dietary flavonoids, glutathione and amino acids could form zinc complexes and facilitate the transport of zinc, with the possible biological implications and potential health benefits of these natural compounds. The study is based on obtaining a molecular conformational structure of the zinc complexes with the lowest possible energy content. The discovery of novel substances that act as zinc ionophores is an attractive research topic that offers exciting opportunities in medicinal chemistry. We propose that these novel complexes could be promising candidates for drug design to provide new solutions for conditions and diseases related to zinc deficiency or impairment derived from the dysregulation of this important metal.
The Model of Systemic Relational Violence: Conceptualizing IPV as a Method of Continual and Enforced Domination
Authors: McLeod David Axlyn , Pharris Angela , Boyles Elizabeth , Winkles Rachael , Stafford Wendy
Published: July 2021

This paper provides a theoretical and historical background of explanatory and descriptive models of domestic, family, and interpersonal violence and introduces a new model that seeks to correct aspects of those models that have been heavily critiqued. The Model of Systemic Relational Violence reconceptualizes violent relationships with coercive control and emotional and psychological violence at the core and more traditional event-based markers of relationship violence as peripheral enforcement tactics in a more extensive system of interpersonal domination. This new model is built on the insights and perspectives of survivors of relational violence and the service providers who support them. It has been developed to be applied in a variety of diverse relationships and contexts.
Tractor Beams, Pressor Beams and Stressor Beams in General Relativity
Authors: Santiago Jessica , Schuster Sebastian , Visser Matt
Published: July 2021

The metrics of general relativity generally fall into two categories: those which are solutions of the Einstein equations for a given source energy-momentum tensor and the “reverse engineered” metrics—metrics bespoke for a certain purpose. Their energy-momentum tensors are then calculated by inserting these into the Einstein equations. This latter approach has found frequent use when confronted with creative input from fiction, wormholes and warp drives being the most famous examples. In this paper, we again take inspiration from fiction and see what general relativity can tell us about the possibility of a gravitationally induced tractor beam. We base our construction on warp drives and show how versatile this ansatz alone proves to be. Not only can we easily find tractor beams (attracting objects), but repulsor/pressor beams are just as attainable, and a generalization to “stressor” beams is seen to present itself quite naturally. We show that all of these metrics would violate various energy conditions. This provides an opportunity to ruminate on the meaning of energy conditions as such and what we can learn about whether an arbitrarily advanced civilization might have access to such beams.
Spherically Symmetric Exact Vacuum Solutions in Einstein-Aether Theory
Authors: Oost Jacob , Mukohyama Shinji , Wang Anzhong
Published: July 2021

We study spherically symmetric spacetimes in Einstein-aether theory in three different coordinate systems, the isotropic, Painlevè-Gullstrand, and Schwarzschild coordinates, in which the aether is always comoving, and present both time-dependent and time-independent exact vacuum solutions. In particular, in the isotropic coordinates we find a class of exact static solutions characterized by a single parameter c14 in closed forms, which satisfies all the current observational constraints of the theory, and reduces to the Schwarzschild vacuum black hole solution in the decoupling limit (c14=0). However, as long as c14≠0, a marginally trapped throat with a finite non-zero radius always exists, and on one side of it the spacetime is asymptotically flat, while on the other side the spacetime becomes singular within a finite proper distance from the throat, although the geometric area is infinitely large at the singularity. Moreover, the singularity is a strong and spacetime curvature singularity, at which both of the Ricci and Kretschmann scalars become infinitely large.
COVID-19 Associated Rhino-Orbital Mucormycosis Complicated by Gangrenous and Bone Necrosis—A Case Report from Honduras
Authors: Palou Elsa Yolanda , Ramos María Auxiliadora , Cherenfant Emec , Duarte Adoni , Fuentes-Barahona Itzel Carolina , Zambrano Lysien I. , Muñoz-Lara Fausto , Montoya-Ramirez Sandra Aracely , Cardona-Ortiz Alex Francisco , Valle-Reconco Jorge Alberto , Montenegro-Idrogo Juan J. , Bonilla-Aldana D. Katterine , Paniz-Mondolfi Alberto E. , Rodriguez-Morales Alfonso J.
Published: July 2021

Background: Mucormycosis is a life-threatening invasive fungal infection most commonly observed in immunocompromised patients. Throughout the COVID-19 pandemic, a growing number of Mucorales associated infections, now termed COVID-19 associated mucormycosis (CAM), have been reported. Despite an increase in fatality reports, no cases of rhino-orbital CAM complicated with gangrenous bone necrosis have been described in the literature to date. Case: A 56-year-old male with a recent COVID-19 diagnosis developed rhino-orbital mucormycosis after 22 days of treatment with dexamethasone. Cultures and histopathological assessment of tissue biopsy confirmed the diagnosis. The patient survived after treatment with amphotericin B. Conclusions: Mucormycosis is an invasive fungal infection affecting mostly immunocompromised patients. Along with the COVID-19 pandemic, the inappropriate use of steroids, in addition to concurrent risk factors, such as diabetes, has led to an increase in the occurrence of these devastating mycoses, leading to the development of severe presentations and complications, as observed in many cases. Early diagnosis and prompt treatment are crucial in order to avoid dissemination and fatal outcomes.
Organizational and legal methods of increasing the efficiency of land use
Authors: Babazhanov A.R. , Inamov B.N.
Published: July 2021

The article analyzes the negative impact on agricultural lands, especially irrigated arable lands and perennial plantations, and gives recommendations for prevention of negative results. Realization of these recommendations can significantly increase the efficiency of land use.
The in-farm land management project for rational land use of the Regional State Unitary Agricultural Enterprise «Staromainskaya», Staromainsky district of Ulyanovsk region
Authors: Khvostov N.V. , Provalova E.V. , Erofeev S.E. , Bannikova E.V. , Mokshina Yu.A.
Published: July 2021

The legal instruments of influence are necessary to change the situation in the field of rational land use and land protection. The authors propose in-farm land management as one of the tools, which can ensure optimal land use and increase the efficiency of agricultural production on the example of RSUAE "Staromainskaya" in Staromainsky district, Ulyanovsk region.
Normative regulation of objects freely placed on municipal lands in Ulan-Ude city
Authors: Kuklina E.E. , Putintseva A.A. , Nimaev B.C.
Published: July 2021

The article considers the problems of normative regulation of objects freely placed on municipal lands in Ulan-Ude city.