bluuebunny/crossref_metadata_2025 huggingface dataset:
CO2—A Crisis or Novel Functionalization Opportunity?
Authors: Lach Daniel , Polanski Jaroslaw , Kapkowski Maciej
Published: February 2022

The growing emission of carbon dioxide (CO2), combined with its ecotoxicity, is the reason for the intensification of research on the new technology of CO2 management. Currently, it is believed that it is not possible to eliminate whole CO2 emissions. However, a sustainable balance sheet is possible. The solution is technologies that use carbon dioxide as a raw material. Many of these methods are based on CO2 methanation, for example, projects such as Power-to-Gas, production of fuels, or polymers. This article presents the concept of using CO2 as a raw material, the catalytic conversion of carbon dioxide to methane, and consideration on CO2 methanation catalysts and their design.
Depositional Structures and Their Reservoir Characteristics in the Wufeng–Longmaxi Shale in Southern Sichuan Basin, China
Authors: Shi Zhensheng , Zhou Tianqi , Wang Hongyan , Sun Shasha
Published: February 2022

This paper documents depositional structures and their reservoir characteristics in the Wufeng–Longmaxi shale from outcrops and cores using thin sections, X-ray powder diffraction (XRD) analysis, carbon–sulfur analyzer, helium porosimeter, decay permeameter, and focused ion beam scanning electron microscope (FIB-SEM). In the study area, clayey and silty laminae abound in the shale. Clayey laminae are rich in bedding parallel fractures, microfractures, and organic pore networks. Silty laminae are rich in isolated inorganic pores and limited amounts of bedding non-parallel fractures. Various inter-lamination of clayey and silty laminae form five depositional structure types which are closely related to the ancient hydrodynamics, paleoredox condition, and sedimentation rate and have significant impacts on shale fractures, microfractures, pore types, pore-size distribution, and porosity. For the paper lamination (PL) and grading lamination composed of siltstone and claystone (GL-SC), organic pores account for 71.6% and 61.4% of the total, and dense bedding parallel and non-parallel fractures intersect to form connective networks. In the interlaminated lamination composed of siltstone and claystone (IL), grading lamination composed of claystone (GL-C) and structureless beds (SB), organic pores merely account for 20% to 51.8% of the total and minor isolated bedding parallel fractures occur. Among five depositional structure types, the PL and GL-SC have the highest porosity, permeability, TOC content, siliceous content, organic pore proportions, and ratios of horizontal to vertical permeability, which help them become shale gas exploration and development targets.
Densification of Yak Manure Biofuel Pellets and Evaluation of Parameters: Effects on Properties
Authors: Liu Jianbiao , Jiang Xuya , Yuan Yanhao , Chen Huanhuan , Zhang Wenbin , Cai Hongzhen , Gao Feng
Published: February 2022

This study was first conducted to comprehensively investigate the potential of yak manure as a raw material to prepare fuel pellets. The effect of different parameters such as binder, pressure, and moisture content on pellet density and diametric compressing strength was investigated using a laboratory single pelleting press unit. Results showed that increasing the pressure can help to obtain high-quality fuel pellets. The pellet properties (density and diametric compressing strength) initially increased and then decreased with moisture content increase. By contrast, binder was not identified to promote density and diametric compressing strength. The parameters were optimized using the response surface method with central composite design to obtain high-quality pellets. In conclusion, the use of yak manure as a raw material to make fuels can be considered a sustainable approach and can effectively be used to fulfill the energy and heating requirements of rural areas.
Single-Stage Buck–Boost Inverters: A State-of-the-Art Survey
Authors: Azizi Mohammadreza , Husev Oleksandr , Vinnikov Dmitri
Published: February 2022

Single-stage buck–boost inverters have attracted the attention of many researchers, due to their ability to increase/decrease the output voltage in one power conversion stage. One of the most important uses of these inverters is in photovoltaic applications, where the voltage of the solar panels varies in a wide range. In recent years, many new inverters have been proposed to improve the performance of existing structures. In this paper, the state of the art of these single-stage buck–boost inverters is discussed. The advantages and disadvantages of each structure are examined from different perspectives, such as the number of components, losses, and performance. Finally, in a general comparison, the properties of all structures are discussed and summarized in a table.
Improvement of PMSG-Based Wind Energy Conversion System Using Developed Sliding Mode Control
Authors: Majout Btissam , Bossoufi Badre , Bouderbala Manale , Masud Mehedi , Al-Amri Jehad F. , Taoussi Mohammed , El Mahfoud Mohammed , Motahhir Saad , Karim Mohammed
Published: February 2022

In recent years, regulating a wind energy conversion system (WECS) under fluctuating wind speed and enhancing the quality of the electricity provided to the grid has become a hard challenge for many academics. The current research provides a better control strategy to decrease the occurrence of chattering phenomena. Combined with the Maximum Power Point Tracking (MPPT) strategy and a pitch angle control, the control is possible to increase the performance and the efficiency of the Permanent Magnet Synchronous Generator (PMSG) based Wind Energy Conversion System. This study attempts initially to regulate the generator and the grid side converter to track the wind speed reference established by the MPPT algorithm. And secondly, to relieve the chattering problem associated with the conventional sliding mode control (CSMC), the proposed sliding mode control (PSMC) is based on a novel smooth continuous switching control. Besides, the suggested sliding mode control stability is confirmed using Lyapunov’s stability function. The complete system was evaluated in the MATLAB/Simulink (MathWorks, Natick, MA, USA) environment using a 2 MW PMSG’s power, under random fluctuations in the wind speed to show the suggested approach’s efficiency and robustness, which was then compared to the CSMC and other common approaches available in the literature. The simulation results reveal that the recommended sliding mode control approach delivers good speed, accuracy, stability, and output current’s ripple.
Automatic Verification Flow Shop Scheduling of Electric Energy Meters Based on an Improved Q-Learning Algorithm
Authors: Peng Long , Li Jiajie , Zhao Jingming , Dang Sanlei , Kong Zhengmin , Ding Li
Published: February 2022

Considering the engineering problem of electric energy meter automatic verification and scheduling, this paper proposes a novel scheduling scheme based on an improved Q-learning algorithm. First, by introducing the state variables and behavior variables, the ranking problem of combinatorial optimization is transformed into a sequential decision problem. Then, a novel reward function is proposed to evaluate the pros and cons of the different strategies. In particular, this paper considers adopting the reinforcement learning algorithm to efficiently solve the problem. In addition, this paper also considers the ratio of exploration and utilization in the reinforcement learning process, and then provides reasonable exploration and utilization through an iterative updating scheme. Meanwhile, a decoupling strategy is introduced to address the restriction of over estimation. Finally, real time data from a provincial electric energy meter automatic verification center are used to verify the effectiveness of the proposed algorithm.
Compensation of Voltage Drops in Trolleybus Supply System Using Battery-Based Buffer Station
Authors: Bartłomiejczyk Mikołaj , Jarzebowicz Leszek , Kohout Jiří
Published: February 2022

This paper analyzes the results of a trial operation of a battery-based buffer station supporting a selected section of trolleybus power supply systems in Pilsen, Czech Republic. The buffer station aims to prevent the catenary from excessive voltage drops in a part of the route that is most remote from the traction substation. Compensation of voltage drops is carried out by continuously measuring the catenary voltage and injecting the current into the catenary if the voltage decreases below a preset value. The effectiveness of such a solution was evaluated by the analysis of numerous experimental recordings, both from the buffer station itself and from trolleybuses in operation. Further on, based on the recordings, a utilized battery capacity was estimated and a control method for decreasing the required capacity was proposed. The optimal capacity analysis, supplemented with an evaluation of required output current rating, was used to define the best storage technology for the considered application.
Powering the WSN Node for Monitoring Rail Car Parameters, Using a Piezoelectric Energy Harvester
Authors: Dziadak Bogdan , Kucharek Mariusz , Starzyński Jacek
Published: February 2022

Monitoring of railroad wagons is important for logistical processes, but above all for safety. One of the key parameters to be monitored is the temperature of the axle box and the bearings in the bogie. The problem with monitoring these parameters is the harsh environment and lack of power supply. In our research, we present a power supply system for a WSN node monitoring the bogie parameters. Knowing the operating conditions, we built a power supply system using a piezoelectric energy harvester. The harvester consists of three piezoelectric elements placed on a double arm pendulum beam. The circuit was modeled in the Comsol Multiphysics environment and then built and tested in laboratory conditions. After confirming energy efficiency, the system was tested on a freight car bogie during an 8 h trip. At typical car vibration frequencies (4–10 Hz), the system is able to generate 73 uW. Combined with an energy buffer of 1000 mAh (3.7 V), it can power a WSN node (based on the nRF5340 chip) for 13 years of operation.
Electromagnetic Interference in a Buried Multiconductor Cable Due to a Dynamic Wireless Power Transfer System
Authors: Cruciani Silvano , Campi Tommaso , Maradei Francesca , Feliziani Mauro
Published: February 2022

The aim of this study is to predict the electromagnetic interference (EMI) effect produced by a dynamic wireless power transfer (DWPT) system on a buried multiconductor signal cable. The short-track DWPT system architecture is here considered with an operating frequency of 85 kHz and maximum power transferred to an EV equal to 10 kW. The EMI source is the DWPT transmitting coil which is activated when a vehicle passes over it. The electric and magnetic fields in the earth produced by the DWPT coil currents are calculated numerically using the finite elements method (FEM). These fields are then used to derive the voltage and current sources that appear in the field-excited multiconductor transmission line (MTL) model, used for the buried shielded cable. The MTL is analyzed considering the first ten harmonics of the current. The currents and voltages at the terminal ends are calculated considering the wireless charging of a single electric vehicle (EV) first, and then the simultaneous charging of 10 EVs which absorb a total power of 100 kW. The preliminary results reveal possible EMI problems in underground cables.
Translating Global Integrated Assessment Model Output into Lifestyle Change Pathways at the Country and Household Level
Authors: Hanmer Clare , Wilson Charlie , Edelenbosch Oreane Y. , van Vuuren Detlef P.
Published: February 2022

Countries’ emission reduction commitments under the Paris Agreement have significant implications for lifestyles. National planning to meet emission targets is based on modelling and analysis specific to individual countries, whereas global integrated assessment models provide scenario projections in a consistent framework but with less granular output. We contribute a novel methodology for translating global scenarios into lifestyle implications at the national and household levels, which is generalisable to any service or country and versatile to work with any model or scenario. Our 5Ds method post-processes Integrated Assessment Model projections of sectoral energy demand for the global region to derive energy-service-specific lifestyle change at the household level. We illustrate the methodology for two energy services (mobility, heating) in two countries (UK, Sweden), showing how effort to reach zero carbon targets varies between countries and households. Our method creates an analytical bridge between global model output and information that can be used at national and local levels, making clear the lifestyle implications of climate targets.
Wind and Solar Power Plant End-of-Life Equipment: Prospects for Management in Ukraine
Authors: Trypolska Galyna , Kurbatova Tetiana , Prokopenko Olha , Howaniec Honorata , Klapkiv Yuriy
Published: February 2022

The Ukrainian renewable energy sector has demonstrated a significant increase in its renewable power capacity, especially for solar and wind power plants. Decommissioning the end-of-life equipment in Ukraine has not yet taken place, but it is only a matter of time. With this in mind, this paper assesses the quantities and the market values of the materials that could potentially be recovered from used solar and wind power plants, and it estimates the impact of their decommissioning on employment in Ukraine. It has been estimated that approximately 8.9 GW of solar power plants, and 3.6 GW of wind power plants, will be decommissioned from 2044 to 2059, and that the cost of the raw materials recovered could reach EUR 421.4 million and EUR 124.6 million in 2021 prices, respectively, compared to the decommissioning costs of EUR 240.1 million and EUR 49.1 million, respectively. Decommissioning renewable power plants will require the creation of new jobs, including 11.6 thousand in the solar industry, and 2.8 thousand in the wind sector. It is essential to amend the legislation in Ukraine, particularly Directive 2012/19/EU, to introduce the principle of the extended producer responsibility to ensure the efficient recycling of renewable power plant end-of-life equipment in Ukraine in the future.
Noises in Double-Differenced GNSS Observations
Authors: Prochniewicz Dominik , Kudrys Jacek , Maciuk Kamil
Published: February 2022

Precise data processing from the Global Navigation Satellite Systems (GNSS) reference station network is mainly based on a combination of double-differenced carrier phase and code observations. This approach allows most of the measurement errors to be removed or reduced and is characterized as the most accurate method. However, creating observation differences between two receivers and two satellites increases the measurement noise of the observations by a factor of 2. As a result, it increases the impact of the incorrect definition of the noise characteristic on the results of the estimation of the unknowns in the positioning model. This is especially important in Multi-GNSS solutions, which integrate measurements from different systems, for which the stochastic parameters of observation may differ significantly. In this paper, the authors prepared a complex analysis of the noise type in double-differenced GNSS (GPS, GLONASS and Galileo) observations, both carrier phase and code ones, with a 1 s sampling interval. The Autocorrelation Function (ACF) method, the Lomb–Scargle (L-S) periodogram method, and the Allan variance (AVAR) method were used. The results that were obtained for the weekly set of measurement data showed that, depending on the system and type of observation, the noise level and its type are significantly different. Among the code measurements, the lowest noise levels were obtained for the GPS C5Q and Galileo C7Q/C8Q observations, with the standard deviations not exceeding ±10 cm, while the noisiest observations were for the GLONASS C1C and C2C signals, which had standard deviations of about ±90 cm and ±45 cm, respectively. For the carrier phase observations, each signal type was characterized by very similar noise levels of ±1.5–3.5 mm. The ACF analysis showed that 1 Hz double-differenced GNSS data can only be treated as being not correlated to time for carrier phase observations; for code observations, an irrelevant autocorrelation may be considered for measurement intervals greater than 20 s. Depending on the GNSS signals, the spectral index k varies in a range from −1.3 to −0.2 for code data and k = 0.0 in the case of phase data. Using the modified Allan deviation (MDEV) allows for specific noise types for each signal and GNSS system to be determined. All of the code observations were characterized by either flicker PM or white PM. In the case of the phase observations, they were all uniquely characterized by white PM (GPS and Galileo or by white PM and flicker PM (GLONASS).
Framework Proposal to Quantify the Contribution of Non-Wood Forest Products to the European Union Forest-Based Bioeconomy
Authors: Di Cori Viola , Robert Nicolas , Franceschinis Cristiano , Pettenella Davide Matteo , Thiene Mara
Published: February 2022

Forest ecosystems provide numerous services that contribute to the wellbeing of societies. However, the lack of knowledge about the benefits of non-wood forest ecosystem services, such as non-wood forest products (NWFPs), hinders efficient forest policy decision-making processes. This study proposes a new extension of the Social Accounting Matrices (SAMs) to capture both the private and the social component of NWFPs. By means of a literature review, we show how extended SAMs can be a suitable approach to explain the causal relationship between the supply of forest ecosystem services, forest-based value chains, and the European bioeconomy. The literature analysis shows that the number of research studies calibrating or using SAMs has been increasing over the last decades. Recent extensions of the SAMs made it possible to evaluate the relationship between human wellbeing and the environment. Moreover, we found applications to the forest sector (limited to timber) and to the supply of some non-wood ecosystem services. We believe the extended SAMs will support decision-makers to design forest strategies that properly account for the benefits NWFPs provide to both the economy and the society.
Combining the Effects of Global Warming, Land Use Change and Dispersal Limitations to Predict the Future Distributions of East Asian Cerris Oaks (Quercus Section Cerris, Fagaceae) in China
Authors: Chen Yuheng , Li Yao , Mao Lingfeng
Published: February 2022

Species shift their ranges in response to climate change (CC). However, they may not be able to track optimal conditions as soon as possible, due to limited dispersal ability or habitat fragmentation, caused by land use and land cover change (LULC). This study aimed to explore the combined impacts of CC, LULC and dispersal limitations on the future range dynamics of Quercus acutissima Carruth., Q. variabilis Blume and Q. chenii Nakai, three dominant Cerris oak tree species in warm-temperate and subtropical deciduous forests of China. We used the Maximum Entropy (Maxent) algorithm to predict the suitable habitats for the years 2050 and 2070, under three representative concentration pathways (RCPs). Habitat fragmentation patterns were examined to assess the influence of LULC. Two migration scenarios (full- and partial-migration) were compared to evaluate the effect of dispersal limitations. We found that annual precipitation (AP), minimum temperature in the coldest month (MTCM) and temperature seasonality (TS) play a key role in determining the present distributions of Q. chenii, while AP, MTCM and annual mean temperature (AMT) contribute the most to the distribution models of Q. variabilis and Q. acutissima. For all the three species, LULC will increase the level of habitat fragmentation and lead to the loss of core areas, while limited dispersal ability will restrict the accessibility of future potentially suitable habitats. Under the scenarios of CC and LULC, the suitable areas of Q. chenii will decrease sharply, while those of Q. variabilis in South China will become unsuitable. Our findings highlight the importance of considering dispersal ability, as well as land use and land cover change, for modeling species’ range shifts in the face of global warming. Our study also provides vital information for guiding the management of East Asian Cerris oaks in China; Q. chenii should be listed as a species requiring priority protection, and the threatened habitats of Q. variabilis should be protected to buffer the impacts of CC and LULC.
Assessment of NO2 Purification by Urban Forests Based on the i-Tree Eco Model: Case Study in Beijing, China
Authors: Gong Cheng , Xian Chaofan , Ouyang Zhiyun
Published: February 2022

Air quality issues caused by nitrogen dioxide (NO2) have become increasingly serious in Chinese cities in recent years. As important urban green infrastructure, urban forests can mitigate gaseous nitrogen pollution by absorbing NO2 through leaf gas exchange. This study investigated spatiotemporal variations in the NO2 removal capacity of urban forests in Beijing city from 2014–2019, based on the i-Tree Eco deposition model. The results show that the annual removal capacity of administrative districts within Beijing city ranged from 14,910 to 17,747 tons, and the largest capacity (2684 tons) was found in the Fangshan district. The annual removal rate of NO2 by urban forests in administrative districts within Beijing was estimated at between 0.50–1.60 g/m2, reaching the highest (1.47 g/m2) in the Mengtougou district. The annual average absorption of NO2 by urban forests can account for 0.14–2.60% of annual total atmospheric NO2 and potentially reduce the NO2 concentration by 0.10–0.34 µg/m3 on average. The results of a principal component analysis suggest that the distribution of urban forests in Beijing is not optimized to maximize their NO2 removal capacity, being higher in suburban areas and lower in urban areas. This study provides insights into botanical NO2 removal capacity in Beijing city to mitigate atmospheric N pollution, addressing the key role of urban forests in improving human wellbeing.
C:N:P Stoichiometry of Plant, Litter and Soil along an Elevational Gradient in Subtropical Forests of China
Authors: Chen Bo , Chen Lyuyi , Jiang Lan , Zhu Jing , Chen Jiajia , Huang Qingrong , Liu Jinfu , Xu Daowei , He Zhongsheng
Published: February 2022

The internal correlation of plant, litter and soil stoichiometric characteristics and their responses to the environment are helpful for revealing nutrient cycling mechanisms. However, few studies have assessed the nutrient relationship between plant, litter and soil and nutrient stock along elevational gradients, which limit the understanding of nutrient relationships in the ecosystem. To gain insight into the forces of nutrient stock and its stoichiometric ecological characteristics along the elevational gradients in forest ecosystem, we investigated the carbon (C), nitrogen (N) phosphorus (P) contents and stoichiometric ratios of dominant plants, litter and soil layers at different elevations (900–1600 m) in Daiyun Mountain. The results showed the following: (1) C, N and P contents showed an increasing order as plant > litter > soil in each elevation of Daiyun Mountain. Dominant plants were limited by N each elevation. C, N and P contents of plants at high elevation were higher than those at low elevation and significant correlations were found between plant and litter TN, TP and air and soil temperature (negative), which conforms to the Temperature-Plant Physiological Hypothesis (TPPH). (2) Significant correlations were found between plant C:N and litter C:N (positive); between litter C:P and soil N:P (positive); and between litter C:P and soil C:N (negative). (3) Elevation and slope were essential environmental factors to the stoichiometric ratio of plant and litter, and pH was the main factor that correlated negatively to soil stoichiometry ratio. Litter provided a link between plant and soil, and there was a coupling among plant, litter and soil nutrients. The results could provide a theoretical basis for understanding the nutrient cycling for the subtropical forest ecosystem of China.
Interspecific Association and Community Stability of Tree Species in Natural Secondary Forests at Different Altitude Gradients in the Southern Taihang Mountains
Authors: Jin Shan-Shan , Zhang Yan-Yan , Zhou Meng-Li , Dong Xiao-Ming , Chang Chen-Hao , Wang Ting , Yan Dong-Feng
Published: February 2022

An interspecific association represents an inter-relatedness of different species in spatial distribution and combined with the altitude factor, is key for revealing the formation and evolution of an ecological community. Therefore, we analyzed the changes in interspecific association and community stability at different altitudes in the southern Taihang Mountains using the variance ratio (VR), χ2 test, association coefficient (AC), percentage of co-occurrence (PC) and Godron stability method. In total, 27 sample plots measuring 20 × 20 m were set up and were divided into lower altitude (700~1100 m), medium altitude (1100~1500 m) and higher altitude areas (1500~1900 m) into. The results showed that the overall interspecies association of communities exhibited an insignificant negative association in both the lower (VR = 0.79, W = 7.15) and higher (VR = 0.81, W = 7.36) altitude areas, while an insignificant positive association was observed in the medium (VR = 1.48, W = 13.34) altitude area. Besides, the χ2 test showed the ratio of positively and negatively correlated species pairs decreased as altitude increased with values of 1.39, 1.22 and 0.95 in the lower, medium and higher altitude areas, respectively. Moreover, the AC and PC indices stated that most species pairs had a weaker association in the three altitude areas, but the AC indices also suggested the number of positive association species pairs was more than that of negative association only in medium altitude area. Meanwhile, the Godron stability method showed the distances from the intersection point to the stable point (20 and 80) were still far away, with values of 22.53, 11.92 and 21.34 in the lower, medium and higher altitude areas, respectively, which indicated an unstable succession stage, though the community appeared steadier in the medium altitude area. This study can provide some guidance for effective afforestation and vegetation restoration.
Adjacency-Information-Entropy-Based Cooperative Name Resolution Approach in ICN
Authors: Li Jiaqi , You Jiali , Deng Haojiang
Published: February 2022

Information-centric networking (ICN) is an emerging network architecture that has the potential to address low-transmission latency and high-reliability requirements in the fifth generation and beyond communication networks (5G/B5G). In the ICN architectures that use the identifier–locator separation mode, a name resolution system (NRS) is an important infrastructure for managing and maintaining the mappings between identifiers and locators. To meet the demands of time-sensitive applications, researchers have developed a distributed local NRS that can provide name resolution service within deterministic latency, which means it can respond to a name resolution request within a latency upper bound. However, processing name resolution requests only locally cannot take full advantage of the potential of the distributed local NRS. In this paper, we propose a name resolution approach, called adjacency-information-entropy-based cooperative name resolution (ACNR). In ACNR, when a name resolution node receives a name resolution request from a user, it can use neighboring name resolution nodes to respond to this request in a parallel processing manner. For this purpose, ACNR uses the information entropy that takes into account the adjacency and latency between name resolution nodes to describe the local structure of nodes efficiently. The proposed approach is extensively validated on simulated networks. Compared with several other approaches, the experiment results show that ACNR can discover more cooperative neighbors in a reasonable communication overhead, and achieve a higher name resolution success rate.
Nutritional Benefits from Fatty Acids in Organic and Grass-Fed Beef
Authors: Davis Hannah , Magistrali Amelia , Butler Gillian , Stergiadis Sokratis
Published: February 2022

Livestock production is under increasing scrutiny as a component of the food supply chain with a large impact on greenhouse gas emissions. Amidst growing calls to reduce industrial ruminant production, there is room to consider differences in meat quality and nutritional benefits of organic and/or pasture-based management systems. Access to forage, whether fresh or conserved, is a key influencing factor for meat fatty acid profile, and there is increasing evidence that pasture access is particularly beneficial for meat’s nutritional quality. These composition differences ultimately impact nutrient supply to consumers of conventional, organic and grass-fed meat. For this review, predicted fatty acid supply from three consumption scenarios were modelled: i. average UK population National Diet and Nutrition Survey (NDNS) (<128 g/week) red meat consumption, ii. red meat consumption suggested by the UK National Health Service (NHS) (<490 g/week) and iii. red meat consumption suggested by the Eat Lancet Report (<98 g/week). The results indicate average consumers would receive more of the beneficial fatty acids for human health (especially the essential omega-3, alpha-linolenic acid) from pasture-fed beef, produced either organically or conventionally.
Flavour Compensation Role of Yeast Strains in Reduced-Salt Dry Sausages: Taste and Odour Profiles
Authors: Li Xiang-Ao , Kong Baohua , Wen Rongxin , Wang Huiping , Li Mengtong , Chen Qian
Published: February 2022

The effects of different yeast strains including Pichia kudriavzevii, Torulaspora delbrueckii, and Debaryomyces hansenii on the taste and odour profiles of reduced-salt dry sausages were explored. Inoculation of P. kudriavzevii and D. hansenii compensated for the lack of saltiness and umami tastes of reduced-salt sausages. Furthermore, inoculation of P. kudriavzevii and T. delbrueckii resulted in an odour profile in the reduced-salt dry sausages that was similar to traditional dry sausages. According to the volatile analysis, the contents of certain alcohols, acids, esters and terpenes were higher in the inoculated sausages. Finally, the sensory evaluation indicated that the inoculation of P. kudriavzevii and D. hansenii contributed positively to the aroma and saltiness of reduced-salt dry sausages. In conclusion, P. kudriavzevii and D. hansenii can be employed as effective starter cultures to compensate for the flavour deficiencies of reduced-salt dry sausages.
Some Generalizations of Different Types of Quantum Integral Inequalities for Differentiable Convex Functions with Applications
Authors: Zhao Dafang , Ali Muhammad Aamir , Luangboon Waewta , Budak Hüseyin , Nonlaopon Kamsing
Published: February 2022

In this paper, we prove a new quantum integral equality involving a parameter, left and right quantum derivatives. Then, we use the newly established equality and prove some new estimates of quantum Ostrowski, quantum midpoint, quantum trapezoidal and quantum Simpson’s type inequalities for q-differentiable convex functions. It is also shown that the newly established inequalities are the refinements of the existing inequalities inside the literature. Finally, some examples and applications are given to illustrate the investigated results.
Gamma-Ray Cosmology and Tests of Fundamental Physics
Authors: Biteau Jonathan , Meyer Manuel
Published: February 2022

The propagation of gamma-rays over cosmological distances is the subject of extensive theoretical and observational research at GeV and TeV energies. The mean free path of gamma-rays in the cosmic web is limited above 100 GeV due to the production of electrons and positrons on the cosmic optical and infrared backgrounds. Electrons and positrons cool in the intergalactic medium while gyrating in its magnetic fields, which could cause either its global heating or the production of lower-energy secondary gamma-rays. The energy distribution of gamma-rays surviving the cosmological journey carries observed absorption features that gauge the emissivity of baryonic matter over cosmic time, constrain the distance scale of ΛCDM cosmology, and limit the alterations of the interaction cross section. Competitive constraints are, in particular, placed on the cosmic star-formation history as well as on phenomena expected from quantum gravity and string theory, such as the coupling to hypothetical axion-like particles or the violation of Lorentz invariance. Recent theoretical and observational advances offer a glimpse of the multi-wavelength and multi-messenger path that the new generation of gamma-ray observatories is about to open.
Cellulosic-Based Conductive Hydrogels for Electro-Active Tissues: A Review Summary
Authors: Gebeyehu Esubalew Kasaw , Sui Xiaofeng , Adamu Biruk Fentahun , Beyene Kura Alemayehu , Tadesse Melkie Getnet
Published: February 2022

The use of hydrogel in tissue engineering is not entirely new. In the last six decades, researchers have used hydrogel to develop artificial organs and tissue for the diagnosis of real-life problems and research purposes. Trial and error dominated the first forty years of tissue generation. Nowadays, biomaterials research is constantly progressing in the direction of new materials with expanded capabilities to better meet the current needs. Knowing the biological phenomenon at the interaction among materials and the human body has promoted the development of smart bio-inert and bio-active polymeric materials or devices as a result of vigorous and consistent research. Hydrogels can be tailored to contain properties such as softness, porosity, adequate strength, biodegradability, and a suitable surface for adhesion; they are ideal for use as a scaffold to provide support for cellular attachment and control tissue shapes. Perhaps electrical conductivity in hydrogel polymers promotes the interaction of electrical signals among artificial neurons and simulates the physiological microenvironment of electro-active tissues. This paper presents a review of the current state-of-the-art related to the complete process of conductive hydrogel manufacturing for tissue engineering from cellulosic materials. The essential properties required by hydrogel for electro-active-tissue regeneration are explored after a short overview of hydrogel classification and manufacturing methods. To prepare hydrogel from cellulose, the base material, cellulose, is first synthesized from plant fibers or generated from bacteria, fungi, or animals. The natural chemistry of cellulose and its derivatives in the fabrication of hydrogels is briefly discussed. Thereafter, the current scenario and latest developments of cellulose-based conductive hydrogels for tissue engineering are reviewed with an illustration from the literature. Finally, the pro and cons of conductive hydrogels for tissue engineering are indicated.
G-Quadruplex Matters in Tissue-Specific Tumorigenesis by BRCA1 Deficiency
Authors: Kim Sanghyun , Hwang Sohyun
Published: February 2022

How and why distinct genetic alterations, such as BRCA1 mutation, promote tumorigenesis in certain tissues, but not others, remain an important issue in cancer research. The underlying mechanisms may reveal tissue-specific therapeutic vulnerabilities. Although the roles of BRCA1, such as DNA damage repair and stalled fork stabilization, obviously contribute to tumor suppression, these ubiquitously important functions cannot explain tissue-specific tumorigenesis by BRCA1 mutations. Recent advances in our understanding of the cancer genome and fundamental cellular processes on DNA, such as transcription and DNA replication, have provided new insights regarding BRCA1-associated tumorigenesis, suggesting that G-quadruplex (G4) plays a critical role. In this review, we summarize the importance of G4 structures in mutagenesis of the cancer genome and cell type-specific gene regulation, and discuss a recently revealed molecular mechanism of G4/base excision repair (BER)-mediated transcriptional activation. The latter adequately explains the correlation between the accumulation of unresolved transcriptional regulatory G4s and multi-level genomic alterations observed in BRCA1-associated tumors. In summary, tissue-specific tumorigenesis by BRCA1 deficiency can be explained by cell type-specific levels of transcriptional regulatory G4s and the role of BRCA1 in resolving it. This mechanism would provide an integrated understanding of the initiation and development of BRCA1-associated tumors.
Rapidly Progressing to ESRD in an Individual with Coexisting ADPKD and Masked Klinefelter and Gitelman Syndromes
Authors: Peces Ramón , Peces Carlos , Mena Rocío , Cuesta Emilio , García-Santiago Fe Amalia , Ossorio Marta , Afonso Sara , Lapunzina Pablo , Nevado Julián
Published: February 2022

Autosomal dominant polycystic kidney disease (ADPKD) is the most common monogenetic hereditary renal disease, promoting end-stage renal disease (ESRD). Klinefelter syndrome (KS) is a consequence of an extra copy of the X chromosome in males. Main symptoms in KS include hypogonadism, tall stature, azoospermia, and a risk of cardiovascular diseases, among others. Gitelman syndrome (GS) is an autosomal recessive disorder caused by SLC12A3 variants, and is associated with hypokalemia, hypomagnesemia, hypocalciuria, normal or low blood pressure, and salt loss. The three disorders have distinct and well-delineated clinical, biochemical, and genetic findings. We here report a male patient with ADPKD who developed early chronic renal failure leading to ESRD, presenting with an intracranial aneurysm and infertility. NGS identified two de novo PKD1 variants, one known (likely pathogenic), and a previously unreported variant of uncertain significance, together with two SLC12A3 pathogenic variants. In addition, cytogenetic analysis showed a 47, XXY karyotype. We investigated the putative impact of this rare association by analyzing possible clinical, biochemical, and/or genetic interactions and by comparing the evolution of renal size and function in the proband with three age-matched ADPKD (by variants in PKD1) cohorts. We hypothesize that the coexistence of these three genetic disorders may act as modifiers with possible synergistic actions that could lead, in our patient, to a rapid ADPKD progression.
Successful Silencing of the Mycotoxin Synthesis Gene TRI5 in Fusarium culmorum and Observation of Reduced Virulence in VIGS and SIGS Experiments
Authors: Tretiakova Polina , Voegele Ralf Thomas , Soloviev Alexander , Link Tobias Immanuel
Published: February 2022

Crops constantly experience various biotic stresses during their life cycle, and Fusarium spp. remain one of the most serious groups of pathogens affecting plants. The ability to manipulate the expression of certain microorganism genes via RNAi creates the opportunity for new-generation dsRNA-based preparations to control a large number of diseases. In this study, we applied virus-induced gene silencing (VIGS), and spray-induced gene silencing (SIGS) to silence the trichothecene-producing gene TRI5 in F. culmorum as a means to reduce its aggressiveness on spring wheat. Treatment of the fungus with dsTRI5RNA in vitro reduced deoxynivalenol (DON) and 3-acetyldeoxynivalenol (3-A-DON) accumulations by 53–85% and 61–87%, respectively, and reduced TRI5 expression by 84–97%. VIGS decreased the proportion of infected wheat spikelets by 73%, but upregulation was observed for TRI5. SIGS on wheat leaves and ears using certain dsTRI5RNA amounts negatively impacted F. culmorum growth. However, when performing in vivo analyses of TRI5 mRNA levels, the upregulation of the gene was determined in the variants where fungal colonization was restricted, suggesting a compensatory reaction of the pathogen to RNAi.
Loss of Heterozygosity in the Tumor DNA of De Novo Diagnosed Patients Is Associated with Poor Outcome for B-ALL but Not for T-ALL
Authors: Risinskaya Natalya , Kozhevnikova Yana , Gavrilina Olga , Chabaeva Julia , Kotova Ekaterina , Yushkova Anna , Isinova Galina , Zarubina Ksenija , Obukhova Tatiana , Kulikov Sergey , Julhakyan Hunan , Sudarikov Andrey , Parovichnikova Elena
Published: February 2022

Despite the introduction of new technologies in molecular diagnostics, one should not underestimate the traditional routine methods for studying tumor DNA. Here we present the evidence that short tandem repeat (STR) profiling of tumor DNA relative to DNA from healthy cells might identify chromosomal aberrations affecting therapy outcome. Tumor STR profiles of 87 adult patients with de novo Ph-negative ALL (40 B-ALL, 43 T-ALL, 4 mixed phenotype acute leukemia (MPAL)) treated according to the “RALL-2016” regimen were analyzed. DNA of tumor cells was isolated from patient bone marrow samples taken at diagnosis. Control DNA samples were taken from the buccal swab or the blood of patients in complete remission. Overall survival (OS) analysis was used to assess the independent impact of the LOH as a risk factor. Of the 87 patients, 21 were found with LOH in various STR loci (24%). For B-ALL patients, LOH (except 12p LOH) was an independent risk factor (OS hazard ratio 3.89, log-rank p-value 0.0395). In contrast, for T-ALL patients, the OS hazard ratio was 0.59 (log-rank p-value 0.62). LOH in particular STR loci measured at the onset of the disease could be used as a prognostic factor for poor outcome in B-ALL, but not in T-ALL.
SINE-Based Phylogenomics Reveal Extensive Introgression and Incomplete Lineage Sorting in Myotis
Authors: Korstian Jennifer M. , Paulat Nicole S. , Platt Roy N. , Stevens Richard D. , Ray David A.
Published: February 2022

Using presence/absence data from over 10,000 Ves SINE insertions, we reconstructed a phylogeny for 11 Myotis species. With nearly one-third of individual Ves gene trees discordant with the overall species tree, phylogenetic conflict appears to be rampant in this genus. From the observed conflict, we infer that ILS is likely a major contributor to the discordance. Much of the discordance can be attributed to the hypothesized split between the Old World and New World Myotis clades and with the first radiation of Myotis within the New World. Quartet asymmetry tests reveal signs of introgression between Old and New World taxa that may have persisted until approximately 8 MYA. Our introgression tests also revealed evidence of both historic and more recent, perhaps even contemporary, gene flow among Myotis species of the New World. Our findings suggest that hybridization likely played an important role in the evolutionary history of Myotis and may still be happening in areas of sympatry. Despite limitations arising from extreme discordance, our SINE-based phylogeny better resolved deeper relationships (particularly the positioning of M. brandtii) and was able to identify potential introgression pathways among the Myotis species sampled.
CDH1 Germline Variants in a Tunisian Cohort with Hereditary Diffuse Gastric Carcinoma
Authors: Ben Aissa-Haj Jihenne , Kabbage Maria , Othmen Houcemeddine , Saulnier Patrick , Kettiti Haifa Tounsi , Jaballah-Gabteni Amira , Ferah Azer , Medhioub Mouna , Khsiba Amal , Mahmoudi Moufida , Maaloul Afifa , Ben Nasr Sonia , Chelbi Emna , Abdelhak Sonia , Boubaker M. Samir , Azzouz Mohamed Mousaddak , Rouleau Etienne
Published: February 2022

Mutational screening of the CDH1 gene is a standard treatment for patients who fulfill Hereditary Diffuse Gastric Cancer (HDGC) testing criteria. In this framework, the classification of variants found in this gene is a crucial step for the clinical management of patients at high risk for HDGC. The aim of our study was to identify CDH1 as well as CTNNA1 mutational profiles predisposing to HDGC in Tunisia. Thirty-four cases were included for this purpose. We performed Sanger sequencing for the entire coding region of both genes and MLPA (Multiplex Ligation Probe Amplification) assays to investigate large rearrangements of the CDH1 gene. As a result, three cases, all with the HDGC inclusion criteria (8.82% of the entire cohort), carried pathogenic and likely pathogenic variants of the CDH1 gene. These variants involve a novel splicing alteration, a missense c.2281G > A detected by Sanger sequencing, and a large rearrangement detected by MLPA. No pathogenic CTNNA1 variants were found. The large rearrangement is clearly pathogenic, implicating a large deletion of two exons. The novel splicing variant creates a cryptic site. The missense variant is a VUS (Variant with Uncertain Significance). With ACMG (American College of Medical Genetics and Genomics) classification and the evidence available, we thus suggest a revision of its status to likely pathogenic. Further functional studies or cosegregation analysis should be performed to confirm its pathogenicity. In addition, molecular exploration will be needed to understand the etiology of the other CDH1- and CTNNA1-negative cases fulfilling the HDGC inclusion criteria.
Multi-Objective Optimization Using Evolutionary Cuckoo Search Algorithm for Evacuation Planning
Authors: Sicuaio Tomé , Niyomubyeyi Olive , Shyndyapin Andrey , Pilesjö Petter , Mansourian Ali
Published: February 2022

Proper emergency evacuation planning is a key to ensuring the safety and efficiency of resources allocation in disaster events. An efficient evacuation plan can save human lives and avoid other effects of disasters. To develop effective evacuation plans, this study proposed a multi-objective optimization model that assigns individuals to emergency shelters through safe evacuation routes during the available periods. The main objective of the proposed model is to minimize the total travel distance of individuals leaving evacuation zones to shelters, minimize the risk on evacuation routes and minimize the overload of shelters. The experimental results show that the Discrete Multi-Objective Cuckoo Search (DMOCS) has better and consistent performance as compared to the standard Multi-Objective Cuckoo Search (MOCS) in most cases in terms of execution time; however, the performance of MOCS is still within acceptable ranges. Metrics and measures such as hypervolume indicator, convergence evaluation and parameter tuning have been applied to evaluate the quality of Pareto front and the performance of the proposed algorithm. The results showed that the DMOCS has better performance than the standard MOCS.