bluuebunny/crossref_metadata_2025 huggingface dataset:
Orthostatic Headache in Children Including Postural Tachycardia Syndrome and Orthostatic Hypotension: A Near-Infrared Spectroscopy Study
Authors: Go Soken , Yamanaka Gaku , Kasuga Akiko , Kanou Kanako , Takamatsu Tomoko , Takeshita Mika , Morishita Natsumi , Morichi Shinichiro , Ishida Yu , Oana Shingo , Kashiwagi Yasuyo , Mitsufuji Takashi , Araki Nobuo , Kawashima Hisashi
Published: December 2020

Background and aim: Although head and/or neck pain attributed to orthostatic hypotension is included in international guidelines, its mechanisms and relevance remain unknown. This study examined the term’s relevance and aimed to elucidate the associated clinical features. Methods: An active stand test was performed to evaluate fluctuations in systemic and cerebral circulation in children and adolescents reporting complaints in the absence of a confirmed organic disorder. The subjects were categorized based on orthostatic headache presence/absence, and their characteristics and test results were compared. Results: Postural tachycardia syndrome was observed in 50.0% of children with, and 55.1% without, orthostatic headache. For orthostatic hypotension, the respective values were 31.3% and 30.6%. A history of migraine was more prevalent in children with orthostatic headaches (64.1% vs. 28.6%; p < 0.01). The observed decrease in the cerebral oxygenated hemoglobin level was larger in children with orthostatic headaches (Left: 6.3 (3.2–9.4) vs. 4.1 (0.8–6.1); p < 0.01, Right: 5.3 (3.1–8.6) vs. 4.0 (0.8–5.9); p < 0.01). Conclusion: Fluctuations in cerebral blood flow were associated with orthostatic headaches in children, suggesting that the headaches are due to impaired intracranial homeostasis. As orthostatic headache can have multiple causes, the term “head and/or neck pain attributed to orthostatic (postural) hypotension” should be replaced with a more inclusive term.
High-Profile VRU Detection on Resource-Constrained Hardware Using YOLOv3/v4 on BDD100K
Authors: Ortiz Castelló Vicent Ortiz , Salvador Igual Ismael Salvador , del Tejo Catalá Omar , Perez-Cortes Juan-Carlos
Published: December 2020

Vulnerable Road User (VRU) detection is a major application of object detection with the aim of helping reduce accidents in advanced driver-assistance systems and enabling the development of autonomous vehicles. Due to intrinsic complexity present in computer vision and to limitations in processing capacity and bandwidth, this task has not been completely solved nowadays. For these reasons, the well established YOLOv3 net and the new YOLOv4 one are assessed by training them on a huge, recent on-road image dataset (BDD100K), both for VRU and full on-road classes, with a great improvement in terms of detection quality when compared to their MS-COCO-trained generic correspondent models from the authors but with negligible costs in forward pass time. Additionally, some models were retrained when replacing the original Leaky ReLU convolutional activation functions from original YOLO implementation with two cutting-edge activation functions: the self-regularized non-monotonic function (MISH) and its self-gated counterpart (SWISH), with significant improvements with respect to the original activation function detection performance. Additionally, some trials were carried out including recent data augmentation techniques (mosaic and cutmix) and some grid size configurations, with cumulative improvements over the previous results, comprising different performance-throughput trade-offs.
Lack of Uniformity in the Israeli Property Tax System 1997–2017
Authors: Perez Avi
Published: December 2020

There are two different forms of property tax systems: value-based tax, which is used in most countries of the world, and area-based tax, which is used mainly in Central and Eastern Europe and developing countries in Africa. Area-based property tax provides more stable and predictable budget revenues. It is simpler to administer and scores worse on equity grounds from the perspective of the ability-to-pay principle of taxation. Against this background, Israel’s property tax system, known as Arnona, is complex, spatially diversified, and causes a lack of uniformity that leads to tax distortion. This paper’s primary purpose is to identify the weaknesses of Israeli property tax from 1997 to 2017 and indicate how to improve the property tax system. This paper is based on case studies from four of the most important cities in Israel: Tel Aviv, Jerusalem, Haifa, and Beersheba, which have four different measurement methods for calculating property tax. Unique data were collected from the Israel Central Bureau of Statistics. According to this analysis, it was found that there are substantial differences in property tax between the four cities over the two decades analyzed. The main weakness is the lack of uniformity of the taxation system; the solution is to unify the measurement of real estate area for tax purposes using drone technology.
Nationwide Susceptibility Mapping of Landslides in Kenya Using the Fuzzy Analytic Hierarchy Process Model
Authors: Zhou Suhua , Zhou Shuaikang , Tan Xin
Published: December 2020

Landslide susceptibility mapping (LSM) is a cost-effective tool for landslide hazard mitigation. To date, no nationwide landslide susceptibility maps have been produced for the entire Kenyan territory. Hence, this work aimed to develop a landslide susceptibility map at the national level in Kenya using the fuzzy analytic hierarchy process method. First, a hierarchical evaluation index system containing 10 landslide contributing factors and their subclasses was established to produce a susceptibility map. Then, the weights of these indexes were determined through pairwise comparisons, in which triangular fuzzy numbers (TFNs) were employed to scale the relative importance based on the opinions of experts. Ultimately, these weights were merged in a hierarchical order to obtain the final landslide susceptibility map. The entire Kenyan territory was divided into five susceptibility levels. Areas with very low susceptibility covered 5.53% of the Kenyan territory, areas with low susceptibility covered 20.58%, areas with the moderate susceptibility covered 29.29%, areas with high susceptibility covered 29.16%, and areas with extremely high susceptibility covered 15.44% of Kenya. The resulting map was validated using an inventory of 425 historical landslides in Kenya. The results indicated that the TFN-AHP model showed a significantly improved performance (AUC = 0.86) compared with the conventional AHP (AUC = 0.72) in LSM for the study area. In total, 31.53% and 29.88% of known landslides occurred within the “extremely high” and “high” susceptibility zones, respectively. Only 8.24% and 1.65% of known landslides fell within the “low” and “very low” susceptibility zones, respectively. The map obtained as a result of this study is beneficial to inform planning and land resource management in Kenya.
Shed Light in the DaRk LineagES of the Fungal Tree of Life—STRES
Authors: Selbmann Laura , Benkő Zsigmond , Coleine Claudia , de Hoog Sybren , Donati Claudio , Druzhinina Irina , Emri Tamás , Ettinger Cassie L. , Gladfelter Amy S. , Gorbushina Anna A. , Grigoriev Igor V. , Grube Martin , Gunde-Cimerman Nina , Karányi Zsolt Ákos , Kocsis Beatrix , Kubressoian Tania , Miklós Ida , Miskei Márton , Muggia Lucia , Northen Trent , Novak-Babič Monika , Pennacchio Christa , Pfliegler Walter P. , Pòcsi Istvàn , Prigione Valeria , Riquelme Meritxell , Segata Nicola , Schumacher Julia , Shelest Ekaterina , Sterflinger Katja , Tesei Donatella , U’Ren Jana M. , Varese Giovanna C. , Vázquez-Campos Xabier , Vicente Vania A. , Souza Emanuel M. , Zalar Polona , Walker Allison K. , Stajich Jason E.
Published: December 2020

The polyphyletic group of black fungi within the Ascomycota (Arthoniomycetes, Dothideomycetes, and Eurotiomycetes) is ubiquitous in natural and anthropogenic habitats. Partly because of their dark, melanin-based pigmentation, black fungi are resistant to stresses including UV- and ionizing-radiation, heat and desiccation, toxic metals, and organic pollutants. Consequently, they are amongst the most stunning extremophiles and poly-extreme-tolerant organisms on Earth. Even though ca. 60 black fungal genomes have been sequenced to date, [mostly in the family Herpotrichiellaceae (Eurotiomycetes)], the class Dothideomycetes that hosts the largest majority of extremophiles has only been sparsely sampled. By sequencing up to 92 species that will become reference genomes, the “Shed light in The daRk lineagES of the fungal tree of life” (STRES) project will cover a broad collection of black fungal diversity spread throughout the Fungal Tree of Life. Interestingly, the STRES project will focus on mostly unsampled genera that display different ecologies and life-styles (e.g., ant- and lichen-associated fungi, rock-inhabiting fungi, etc.). With a resequencing strategy of 10- to 15-fold depth coverage of up to ~550 strains, numerous new reference genomes will be established. To identify metabolites and functional processes, these new genomic resources will be enriched with metabolomics analyses coupled with transcriptomics experiments on selected species under various stress conditions (salinity, dryness, UV radiation, oligotrophy). The data acquired will serve as a reference and foundation for establishing an encyclopedic database for fungal metagenomics as well as the biology, evolution, and ecology of the fungi in extreme environments.
The Circadian Clock—A Molecular Tool for Survival in Cyanobacteria
Authors: Kim Pyonghwa , Kaur Manpreet , Jang Hye-In , Kim Yong-Ick
Published: December 2020

Cyanobacteria are photosynthetic organisms that are known to be responsible for oxygenating Earth’s early atmosphere. Having evolved to ensure optimal survival in the periodic light/dark cycle on this planet, their genetic codes are packed with various tools, including a sophisticated biological timekeeping system. Among the cyanobacteria is Synechococcus elongatus PCC 7942, the simplest clock-harboring organism with a powerful genetic tool that enabled the identification of its intricate timekeeping mechanism. The three central oscillator proteins—KaiA, KaiB, and KaiC—drive the 24 h cyclic gene expression rhythm of cyanobacteria, and the “ticking” of the oscillator can be reconstituted inside a test tube just by mixing the three recombinant proteins with ATP and Mg2+. Along with its biochemical resilience, the post-translational rhythm of the oscillation can be reset through sensing oxidized quinone, a metabolite that becomes abundant at the onset of darkness. In addition, the output components pick up the information from the central oscillator, tuning the physiological and behavioral patterns and enabling the organism to better cope with the cyclic environmental conditions. In this review, we highlight our understanding of the cyanobacterial circadian clock and discuss how it functions as a molecular chronometer that readies the host for predictable changes in its surroundings.
Virus-Like Particle Mediated CRISPR/Cas9 Delivery for Efficient and Safe Genome Editing
Authors: Lyu Pin , Wang Luxi , Lu Baisong
Published: December 2020

The discovery of designer nucleases has made genome editing much more efficient than before. The designer nucleases have been widely used for mechanistic studies, animal model generation and gene therapy development. However, potential off-targets and host immune responses are issues still need to be addressed for in vivo uses, especially clinical applications. Short term expression of the designer nucleases is necessary to reduce both risks. Currently, various delivery methods are being developed for transient expression of designer nucleases including Zinc Finger Nuclease (ZNF), Transcription Activator-Like Effector Nuclease (TALEN) and Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated (CRISPR/Cas). Recently, virus-like particles are being used for gene editing. In this review, we will talk through commonly used genome editing nucleases, discuss gene editing delivery tools and review the latest literature using virus-like particles to deliver gene editing effectors.
Complex Ginzburg–Landau Equation with Generalized Finite Differences
Authors: Salete Eduardo , Vargas Antonio M. , García Ángel , Negreanu Mihaela , Benito Juan J. , Ureña Francisco
Published: December 2020

In this paper we obtain a novel implementation for irregular clouds of nodes of the meshless method called Generalized Finite Difference Method for solving the complex Ginzburg–Landau equation. We derive the explicit formulae for the spatial derivative and an explicit scheme by splitting the equation into a system of two parabolic PDEs. We prove the conditional convergence of the numerical scheme towards the continuous solution under certain assumptions. We obtain a second order approximation as it is clear from the numerical results. Finally, we provide several examples of its application over irregular domains in order to test the accuracy of the explicit scheme, as well as comparison with other numerical methods.
A Navier–Stokes-Type Problem with High-Order Elliptic Operator and Applications
Authors: Ragusa Maria Alessandra , Shakhmurov Veli B.
Published: December 2020

The existence, uniqueness and uniformly Lp estimates for solutions of a high-order abstract Navier–Stokes problem on half space are derived. The equation involves an abstract operator in a Banach space E and small parameters. Since the Banach space E is arbitrary and A is a possible linear operator, by choosing spaces E and operators A, the existence, uniqueness and Lp estimates of solutions for numerous classes of Navier–Stokes type problems are obtained. In application, the existence, uniqueness and uniformly Lp estimates for the solution of the Wentzell–Robin-type mixed problem for the Navier–Stokes equation and mixed problem for degenerate Navier–Stokes equations are established.
Pharmacogenetics Update on Biologic Therapy in Psoriasis
Authors: Muñoz-Aceituno Ester , Martos-Cabrera Luisa , Ovejero-Benito María Carmen , Reolid Alejandra , Abad-Santos Francisco , Daudén Esteban
Published: December 2020

Background and objectives: Psoriasis is a chronic immune-mediated skin disease caused by several complex factors, both environmental and genetic, many of which are still not fully understood. Nowadays, several groups of biological drugs are being used for psoriasis treatment. Although these therapies are very effective, they show significant variability in efficacy among individuals. Therefore, there is a need for biomarkers to predict treatment outcomes in order to guide personalized therapeutic decisions. Pharmacogenetics is the study of variations in DNA sequences related to drug response. Materials and Methods: In this article, we review pharmacogenetics studies on the treatment of moderate-to-severe psoriasis focusing on anti-interleukin (IL) 12/23 (ustekinumab) and anti-IL17 drugs (secukinumab and ixekizumab), as well as recent studies concerning anti-TNF drugs. Results: Several polymorphisms have been studied over the years in reference to anti-TNF drugs; some of the most recent studies included the performance of a genome-wide association study (GWAS) and pharmacogenetics studies focused on the optimization of a treatment regimen. Various polymorphisms in different genes have been related to ustekinumab response; among them, the most commonly studied is the HLA-C*06:02 allele. Conclusions: Although not confirmed in some studies, most studies have shown that patients carrying this allele present a significantly higher response rate to ustekinumab. Some polymorphisms have been studied in patients treated with anti-IL17 drugs, mostly related to secukinumab; however, up to now, no association has been found between any of these polymorphisms and response. Nevertheless, further studies involving larger cohorts are needed in order to confirm these results before the implementation of this biomarker in clinical practice.
Effect of Cooling Path on Microstructures and Hardness of Hot-Stamped Steel
Authors: Xu Yaowen , Ji Qiumei , Yang Gengwei , Bao Siqian , Zhao Gang , Miao Xiaodong , Mao Xinping
Published: December 2020

The final mechanical properties of hot-stamped steel are determined by the microstructures which are greatly influenced by the cooling process after hot stamping. This research studied the effect of the cooling path on the microstructures and hardness of 22MnB5 hot-stamped steel. The cooling path was divided into continuous and discontinuous (primary and secondary) processes. After cooling, the Vickers hardness along the thickness of the specimens was measured. The results indicate that, for a continuous cooling process, there was a critical cooling rate of 25 °C/s to obtain fully martensitic microstructure. For the discontinuous cooling process, the slower was the cooling rate, the higher was the degree of auto-tempering that occurred, and the greater was the amount of carbides that formed, regardless of the primary or secondary cooling rate. When the cooling rate was lower than the critical value, a higher primary cooling rate suppressed the auto-tempering of lath martensite and increased the quenched hardness. By contrast, the hardness was not sensitive to the cooling rate when it exceeded the critical value.
Fe-6.5 wt%Si Powder Cores with Low Core Loss by Optimizing Particle Size Distribution
Authors: Huang JianJun , Jiao Lixin , Yang Yu , Dong Yaqiang , Zhang Yiqun , Chang Liang , Gong Mengji , Li Jiawei , He Aina , Wang Xinmin
Published: December 2020

The effect of different particle size distribution of Fe-6.5 wt%Si powder on the microstructure and soft magnetic properties of the corresponding soft magnetic powder cores (SMPCs) was investigated. By optimizing particle size distribution, the density of SMPCs increased and the total core loss significantly decreased. According to the result of loss separation, density of SMPCs is inversely proportional to hysteresis loss, while with increasing the content of the fine particles, the eddy current loss significantly decreased. It was found that with magnetic powder of particle size-grading as 10%, 10%, 60%, and 20% for particles with size between −75 to +38, −38 to +23, −23 to +13, and −13 μm, respectively, the Fe-6.5 wt%Si SMPCs exhibit optimal comprehensive magnetic performances with the effective permeability of about 60, the percent permeability at 100 Oe is up to 70%, and the lowest core loss of 553 mW/cm3.
Digital Approach for the Rehabilitation of the Edentulous Maxilla with Pterygoid and Standard Implants: The Static and Dynamic Computer-Aided Protocols
Authors: Franchina Alessio , Stefanelli Luigi Vito , Gorini Simone , Fedi Simone , Lizio Giuseppe , Pellegrino Gerardo
Published: December 2020

A full-arch rehabilitation of the edentulous upper jaw without grafting procedures exploits the residual alveolar or the basal bone, with the necessity of long implants placed with a particular orientation. The precision in planning and placing the fixtures is fundamental to avoid clinical problems and to allow an acceptable connection with the prosthesis. The computer-aided implantology resulted in more accuracy than the traditional one, with a high standard of correspondence between the virtual project and the real outcome. This paper reports about the two different digital protocols, static and dynamic, as support to implant-borne prosthetic rehabilitation of edentulous maxillae. Two pterygoid and two/four anterior standard implants were seated in both cases by two different operators, without flap raising, and immediately loaded. This approach avoided the posterior cantilever by-passing the maxillary sinus and was adequately planned and realized without any surgical or prosthetic error. The two digital flow-charts were described step by step, underlining each other’s advantages and drawbacks compared to a free-hand approach.
The Control of Zoonotic Soil-Transmitted Helminthoses Using Saprophytic Fungi
Authors: Viña Cándido , Silva María Isabel , Palomero Antonio Miguel , Voinot Mathilde , Vilá María , Hernández José Ángel , Paz-Silva Adolfo , Sánchez-Andrade Rita , Cazapal-Monteiro Cristiana Filipa , Arias María Sol
Published: December 2020

Soil-transmitted helminths (STHs) are parasites transmitted through contact with soil contaminated with their infective eggs/larvae. People are infected by exposure to human-specific species or animal species (zoonotic agents). Fecal samples containing eggs of Ascaris suum or Lemurostrongylus sp. were sprayed with spores of the soil saprophytic filamentous fungi Clonostachys rosea (CR) and Trichoderma atrobrunneum (TA). The antagonistic effect was assessed by estimating the viability of eggs and their developmental rate. Compared to the controls (unexposed to fungi), the viability of the eggs of A. suum was halved in CR and decreased by two thirds in TA, while the viability of the eggs of Lemurostrongylus sp. was reduced by one quarter and one third in CR and TA treatments, respectively. The Soil Contamination Index (SCI), defined as the viable eggs that attained the infective stage, reached the highest percentages for A. suum in the controls after four weeks (66%), with 21% in CL and 11% in TA. For Lemurostrongylus sp., the values were 80%, 49%, and 41% for control, CR and TA treatments, respectively. We concluded that spreading spores of C. rosea or T. atrobrunneum directly onto the feces of animal species represents a sustainable approach under a One Health context to potentially reduce the risk of zoonotic STHs in humans.
Molecular Phylogeny and Phylogeography of Potentilla multifida L. agg. (Rosaceae) in Northern Eurasia with Special Focus on Two Rare and Critically Endangered Endemic Species, P. volgarica and P. eversmanniana
Authors: Schanzer Ivan A. , Fedorova Alina V. , Shelepova Olga V. , Suleymanova Guzyaliya F.
Published: December 2020

The results of a molecular genetic study of Potentilla multifida agg. using two plastid markers (ndhC-trnV and psbA-trnH) and a nuclear ITS marker suggested that this group comprises a number of relatively young and incompletely differentiated species widely distributed in Northern Eurasia. The sequences were analyzed using tree-based (maximum likelihood) and network-based (statistical parsimony network) approaches. The plastid data suggested incomplete lineage sorting, characteristic of the group as a whole. The nuclear ITS results demonstrated quite a different pattern, with mostly conspecific accessions shaping monophyletic clades. The majority of the Potentilla sect. Multifidae species studied possess few, usually closely related plastid haplotypes, or are even monomorphic. In contrast, P. volgarica, a narrow endemic from the Volga River valley, presents plastid haplotypes belonging to two distantly related groups. Such a pattern of genetic diversity in P. volgarica may be explained by a long persistence of the species within an extremely small distribution range, on the right bank of the Volga River, most likely representing a contemporary refugium. The genealogy of plastid markers in P. volgarica suggests that this species is ancestral to P.eversmanniana, another narrow endemic from the S Urals.
The Role of Symbiotic Microorganisms, Nutrient Uptake and Rhizosphere Bacterial Community in Response of Pea (Pisum sativum L.) Genotypes to Elevated Al Concentrations in Soil
Authors: Belimov Andrey A. , Shaposhnikov Alexander I. , Syrova Darya S. , Kichko Arina A. , Guro Polina V. , Yuzikhin Oleg S. , Azarova Tatiana S. , Sazanova Anna L. , Sekste Edgar A. , Litvinskiy Vladimir A. , Nosikov Vladimir V. , Zavalin Aleksey A. , Andronov Evgeny E. , Safronova Vera I.
Published: December 2020

Aluminium being one of the most abundant elements is very toxic for plants causing inhibition of nutrient uptake and productivity. The aim of this study was to evaluate the potential of microbial consortium consisting of arbuscular mycorrhizal fungus (AMF), rhizobia and PGPR for counteracting negative effects of Al toxicity on four pea genotypes differing in Al tolerance. Pea plants were grown in acid soil supplemented with AlCl3 (pHKCl = 4.5) or neutralized with CaCO3 (pHKCl = 6.2). Inoculation increased shoot and/or seed biomass of plants grown in Al-supplemented soil. Nodule number and biomass were about twice on roots of Al-treated genotypes after inoculation. Inoculation decreased concentrations of water-soluble Al in the rhizosphere of all genotypes grown in Al-supplemented soil by about 30%, improved N2 fixation and uptake of fertilizer 15N and nutrients from soil, and increased concentrations of water-soluble nutrients in the rhizosphere. The structure of rhizospheric microbial communities varied to a greater extent depending on the plant genotype, as compared to soil conditions and inoculation. Thus, this study highlights the important role of symbiotic microorganisms and the plant genotype in complex interactions between the components of the soil-microorganism-plant continuum subjected to Al toxicity.
Removal of Nitrogen and Phosphorus from Thickening Effluent of an Urban Wastewater Treatment Plant by an Isolated Green Microalga
Authors: Baldisserotto Costanza , Demaria Sara , Accoto Ornella , Marchesini Roberta , Zanella Marcello , Benetti Linda , Avolio Francesco , Maglie Michele , Ferroni Lorenzo , Pancaldi Simonetta
Published: December 2020

Microalgae are photosynthetic microorganisms and are considered excellent candidates for a wide range of biotechnological applications, including the removal of nutrients from urban wastewaters, which they can recover and convert into biomass. Microalgae-based systems can be integrated into conventional urban wastewater treatment plants (WW-TP) to improve the water depuration process. However, microalgal strain selection represents a crucial step for effective phytoremediation. In this work, a microalga isolated from the effluent derived from the thickening stage of waste sludge of an urban WW-TP was selected and tested to highlight its potential for nutrient removal. Ammonium and phosphate abatements by microalgae were evaluated using both the effluent and a synthetic medium in a comparative approach. Parallelly, the isolate was characterized in terms of growth capability, morphology, photosynthetic pigment content and photosystem II maximum quantum yield. The isolated microalga showed surprisingly high biomass yield and removal efficiency of both ammonium and phosphate ions from the effluent but not from the synthetic medium. This suggests its clear preference to grow in the effluent, linked to the overall characteristics of this matrix. Moreover, biomass from microalgae cultivated in wastewater was enriched in photosynthetic pigments, polyphosphates, proteins and starch, but not lipids, suggesting its possible use as a biofertilizer.
The Role of Vegetation on the Dynamics of Water and Fire in the Cerrado Ecosystems: Implications for Management and Conservation
Authors: Klink Carlos A. , Sato Margarete N. , Cordeiro Giovanna G. , Ramos Maria Inês M.
Published: December 2020

The Cerrado is the richest savanna and is undergoing one of the planet’s most rapid land transformations for pasture and agriculture; around 45% of the biome has been deforested. Agriculture is of strategic importance to Brazil, but it also modifies ecosystems and jeopardizes habitats and biodiversity. Well-managed agricultural lands can have a favorable impact on environmental conservation. In this paper, we reviewed our current knowledge about water ecology and fire management to show that an ecosystem services perspective can bring about a conciliation of agriculture production with conservation by supporting effective land use decision-making and the optimization of public policy. The landscape/watershed scale seems to be the most relevant for decision-making on how to achieve production and conservation results. This scale appears to be an appropriate level for engaging with stakeholders. Fire frequency and timing (season) combination are determinant of individuals’ survivorship. The combination determines vegetation recovery, and it is important to maintain high biodiversity, especially for the herbaceous layer, but it is a limitation to woody vegetation recovery. A pragmatic and conciliatory land use agenda must be based on scientific knowledge and support innovative decision-making solutions for policy-makers and stakeholders, particularly farmers and donors.
Peptone-Induced Physio-Biochemical Modulations Reduce Cadmium Toxicity and Accumulation in Spinach (Spinacia oleracea L.)
Authors: Emanuil Naila , Akram Muhammad Sohail , Ali Shafaqat , El-Esawi Mohamed A. , Iqbal Muhammad , Alyemeni Mohammed Nasser
Published: December 2020

The accumulation of cadmium (Cd) in edible plant parts and fertile lands is a worldwide problem. It negatively influences the growth and productivity of leafy vegetables (e.g., spinach, Spinacia oleracea L.), which have a high tendency to radially accumulate Cd. The present study investigated the influences of peptone application on the growth, biomass, chlorophyll content, gas exchange parameters, antioxidant enzymes activity, and Cd content of spinach plants grown under Cd stress. Cd toxicity negatively affected spinach growth, biomass, chlorophyll content, and gas exchange attributes. However, it increased malondialdehyde (MDA), hydrogen peroxide (H2O2), electrolyte leakage (EL), proline accumulation, ascorbic acid content, Cd content, and activity of antioxidant enzymes such as superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) in spinach plants. The exogenous foliar application of peptone increased the growth, biomass, chlorophyll content, proline accumulation, and gas exchange attributes of spinach plants. Furthermore, the application of peptone decreased Cd uptake and levels of MDA, H2O2, and EL in spinach by increasing the activity of antioxidant enzymes. This enhancement in plant growth and photosynthesis might be due to the lower level of Cd accumulation, which in turn decreased the negative impacts of oxidative stress in plant tissues. Taken together, the findings of the study revealed that peptone is a promising plant growth regulator that represents an efficient approach for the phytoremediation of Cd-polluted soils and enhancement of spinach growth, yield, and tolerance under a Cd-dominant environment.
New Eudesmane-Type Sesquiterpene Glycosides from the Leaves of Aster koraiensis
Authors: Kim Ji-Young , Seo Young Hye , Lee Im-Ho , Choi He Yun , Kwon Hak Cheol , Choi Jung-Hye , Lee Jun , Jang Dae Sik
Published: December 2020

Four new eudesmane-type sesquiterpenoids, (1R,5S,6R,7S,9S,10S)-1,6,9-trihydroxy-eudesm-3-ene-1,6-di-O-β-d-glucopyranoside (1), (1R,5S,6S,7R,9S,10S)-1,6,9,11-tetrahydroxy-eudesm-3-ene-1,6-di-O-β-d-glucopyranoside (3), (1R,5S,6R,7S,9S,10R)-9-O-(Z-p-coumaroyl)-1,6,9-trihydroxy-eudesm-3-ene-6-O-β-d-glucopyranoside (6), and (1R,5S,6R,7S,9S,10R)-9-O-(E-feruloyl)-1,6,9-trihydroxy-eudesm-3-ene-6-O-β-d-glucopyranoside (7), were isolated from a 95% EtOH extract of the leaves of Aster koraiensis by repeated chromatography. Moreover, three sesquiterpenoids (2, 4, and 5) and two caffeoylquinic acids (8 and 9) having previously known chemical structures were isolated during the isolation procedure. The four new compounds (1, 3, 6, and 7) were elucidated by spectroscopic data (1D- and 2D-NMR, MS, and ECD) interpretation and hydrolysis. Moreover, the absolute configurations of 2, 4, and 5 were determined for the first time in this study. The compounds isolated were tested for their viability on nitric oxide (NO) and prostaglandin E2 (PGE2) production on LPS-stimulated RAW 264.7 cells. Among them, only 7 presented weak inhibitory effects on both NO and PGE2 production.
Antifungal Effect of Volatile Organic Compounds from Bacillus velezensis CT32 against Verticillium dahliae and Fusarium oxysporum
Authors: Li Xinxin , Wang Xiuhong , Shi Xiangyuan , Wang Baoping , Li Meiping , Wang Qi , Zhang Shengwan
Published: December 2020

The present study focuses on the inhibitory effect of volatile metabolites released by Bacillus velezensis CT32 on Verticillium dahliae and Fusarium oxysporum, the causal agents of strawberry vascular wilt. The CT32 strain was isolated from maize straw compost tea and identified as B. velezensis based on 16S rRNA gene sequence analysis. Bioassays conducted in sealed plates revealed that the volatile organic compounds (VOCs) produced by the strain CT32 possessed broad-spectrum antifungal activity against eight phytopathogenic fungi. The volatile profile of strain CT32 was obtained by headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS). A total of 30 volatile compounds were identified, six of which have not previously been detected in bacteria or fungi: (Z)-5-undecene, decyl formate, 2,4-dimethyl-6-tert-butylphenol, dodecanenitrile, 2-methylpentadecane and 2,2’,5,5’-tetramethyl-1,1’-biphenyl. Pure compounds were tested in vitro for their inhibitory effect on the mycelial growth of V. dahliae and F. oxysporum. Decanal, benzothiazole, 3-undecanone, 2-undecanone, 2-undecanol, undecanal and 2,4-dimethyl-6-tert-butylphenol showed high antifungal activity, with benzothiazole and 2,4-dimethyl-6-tert-butylphenol being the most potent compounds. These results indicate that the VOCs produced by B. velezensis CT32 have the potential to be used as a biofumigant for management of vascular wilt pathogens.
Pharmacoeconomic Analysis of Hemophilia Care in Romania
Authors: Serban Petre , Vlaicu Brigitha , Serban Margit , Ursu Cristina Emilia , Traila Adina , Jinca Cristian , Patrascu Jenel Marian , Andrei Daniel , Kozma Andrei , Arghirescu Teodora Smaranda
Published: December 2020

Hemophilia, a congenital X linked disease, has the serious burden of bleeding, requiring life-long replacement with coagulation factors (CF). In the present day, there is a continuously improving treatment for this condition. Objective: Our observational, cross-sectional study aims at finding out whether a prophylactic replacement with CF is affordable from the point of view of its cost-effectiveness in our country. Material and methods: A cohort of 122 persons with hemophilia were included in this patient-reported outcome survey, and they answered a questionnaire consisting of 56 items, focused on 4 domains—socio-demographic, medical, quality of health/life and cost/cost-effectiveness. Results and discussion: The markers for quality of health/life were correlated with the direct and indirect costs of care, comparing subgroup 1 of patients with prophylactic vs. subgroup 2 with on-demand replacement. Based on the incremental quality adjusted life years and the incremental costs, we calculated the incremental cost-effectiveness ratio (ICER) proving that prophylaxis is more cost-effective than on-demand replacement on a long time basis. Conclusions: The ICER is a threshold recommending the reimbursement of costs for a life-long prophylactic replacement in our country.
Iron-Based Catalytically Active Complexes in Preparation of Functional Materials
Authors: Rydel-Ciszek Katarzyna , Pacześniak Tomasz , Zaborniak Izabela , Błoniarz Paweł , Surmacz Karolina , Sobkowiak Andrzej , Chmielarz Paweł
Published: December 2020

Iron complexes are particularly interesting as catalyst systems over the other transition metals (including noble metals) due to iron’s high natural abundance and mediation in important biological processes, therefore making them non-toxic, cost-effective, and biocompatible. Both homogeneous and heterogeneous catalysis mediated by iron as a transition metal have found applications in many industries, including oxidation, C-C bond formation, hydrocarboxylation and dehydration, hydrogenation and reduction reactions of low molecular weight molecules. These processes provided substrates for industrial-scale use, e.g., switchable materials, sustainable and scalable energy storage technologies, drugs for the treatment of cancer, and high molecular weight polymer materials with a predetermined structure through controlled radical polymerization techniques. This review provides a detailed statement of the utilization of homogeneous and heterogeneous iron-based catalysts for the synthesis of both low and high molecular weight molecules with versatile use, focusing on receiving functional materials with high potential for industrial application.
Flow and Diffusion Characteristics of Typical Halon Extinguishing Agent Substitute under Different Release Pressures
Authors: Jin Jiaming , Pan Renming , Chen Ruiyu , Xu Xiaokang , Li Quanwei
Published: December 2020

To provide guidance towards reducing the weight of the HFC-125 storage vessel by reducing the release pressure and to reveal the effects of release pressure on the extinguishing efficiency of HFC-125, we investigated the flow and diffusion characteristics of HFC-125 under six release pressures in the present study. The influence of release pressure on the degree of superheat, injection duration, pressure loss, jet angle, and concentration distribution were analyzed. Results show that the degree of superheat and the injection duration both decreased with the release pressure. The bubble expansion in the HFC-125 could slow down the pressure decrease in the storage vessel. The flow process in the pipeline can be divided into three phases: pipeline filling, stable flow, and mixed gases release. Both of the maximum and mean values of the pipeline pressure loss increased with the release pressure. The maximum concentration value decreased with the increase of the distance from the nozzle. The maximum concentration value in the near field from the nozzle increased with the release pressure. The concentration and holding time (duration above 17.6% volume concentration) of HFC-125 in the near field from the nozzle met the requirements of minimum performance standards (MPS) for HFC-125.
Qawwali Routes: Notes on a Sufi Music’s Transformation in Diaspora
Authors: Gaind-Krishnan Sonia
Published: December 2020

In recent years, alongside the concurrent rise of political Islam and reactionary state policies in India, Sufism has been championed as an “acceptable” form of Islam from neoliberal perspectives within India and the Western world. Sufism is noted as an arena of spiritual/religious practice that highlights musical routes to the Divine. Among Chishti Sufis of South Asia, that musical pathway is qawwali, a song form that been in circulation for over seven centuries, and which continues to maintain a vibrant sonic presence on the subcontinent, both in its ritual usage among Sufis and more broadly in related folk and popular iterations. This paper asks, what happens to qawwali as a song form when it circulates in diaspora? While prominent musicians such as the Sabri Brothers and Nusrat Fateh Ali Khan exposed audiences in the West to the sounds of qawwali, in recent years, non-hereditary groups of musicians based in the US and UK have begun to perform songs from the qawwali repertoire. In the traditional setting, textual meaning is paramount; this paper asks, how can performers transmute the affective capacity of qawwali in settings where semantic forms of communication may be lost? How do sonic and metaphorical voices lend themselves to the circulation of sound-centered meaning? Through a discussion of the Sufi sublime, this paper considers ways sonic materials stitch together the diverse cloth of the South Asian community in diaspora.
“His Soul Is Weeping inside That He Cannot Bury the Dead as before.” Plague and Rebellion in Debrecen (Hungary), 1739–1742
Authors: Sz. Kristóf Ildikó
Published: December 2020

This is a historical anthropological study of a period of social and religious tensions in a Calvinist city in the Kingdom of Hungary in the first half of the 18th century. The last and greatest plague epidemic to devastate Hungary and Transylvania between cca. 1738 and 1743 led to a clash of different opinions and beliefs on the origin of the plague and ways of fighting it. Situated on the Great Hungarian Plain, the city of Debrecen saw not only frequent violations of the imposed lockdown measures among its inhabitants but also a major uprising in 1739. The author examines the historical sources (handwritten city records, written and printed regulations, criminal proceedings, and other documents) to be found in the Debrecen city archives, as well as the writings of the local Calvinist pastors published in the same town. The purpose of the study is to outline the main directions of interpretation concerning the plague and manifest in the urban uprising. According to the findings of the author, there was a stricter and chronologically earlier direction, more in keeping with local Puritanism in the second half of the 17th century, and there was also a more moderate and later one, more in line with the assumptions and expectations of late 18th-century medical science. While the former set of interpretations seems to have been founded especially on a so-called “internal” cure (i.e., religious piety and repentance), the latter proposed mostly “external” means (i.e., quarantine measures and herbal medicine) to avoid the plague and be rid of it. There seems to have existed, however, a third set of interpretations: that of folk beliefs and practices, i.e., sorcery and magic. According to the files, a number of so-called “wise women” also attempted to cure the plague-stricken by magical means. The third set of interpretations and their implied practices were not tolerated by either of the other two. The author provides a detailed micro-historical analysis of local events and the social and religious discourses into which they were embedded.
Enhanced Quarter Spherical Acoustic Energy Harvester Based on Dual Helmholtz Resonators
Authors: Ji Xincun , Yang Lei , Xue Zhicheng , Deng Licheng , Wang Debo
Published: December 2020

An enhanced quarter-spherical acoustic energy harvester (AEH) with dual Helmholtz resonators was designed in this work. Compared with the previous research, this AEH can harvest multi-directional acoustic energy, has a widened resonance frequency band, and has an improved energy conversion efficiency. When the length of resonator’s neck is changed, the acoustic resonant frequency of the two resonators is different. The theoretical models of output voltage and output power were studied, and the relationship of output performance with frequency was obtained. The results showed that this AEH can operate efficiently in a frequency band of about 470 Hz. Its output voltage was found to be about 28 mV, and its output power was found to be about 0.05 μW. The power density of this AEH was found to be about 12.7 µW/cm2. Therefore, this AEH could be widely used in implantable medical devices such as implantable cardiac pacemakers, cochlear implants, and retinal prosthesis.
Analysis of Contact Stresses and Rolling Resistance of Truck-Bus Tyres under Different Working Conditions
Authors: Guo Minrui , Li Xiangwen , Ran Maoping , Zhou Xinglin , Yan Yuan
Published: December 2020

In this work, to analyse the changing characteristics of contact stresses in the tyre–pavement interface and the functional relationship between rolling resistance and the working conditions of truck-bus tyres, a three-dimensional tyre–pavement model is established and used to predict the distribution of contact stresses and rolling resistance under different working conditions of the tyre, comprising various tyre loads, inflation pressures, and velocities. Results show that the magnitude relationship between transverse and longitudinal contact stresses is related to rolling conditions, and overload and low tyre pressure are important contributors to the wear of the tyre shoulder. In addition, the proposed exponential equation presents a method that can be used to forecast rolling resistance related to the working conditions of the truck-bus tyre, and a similar method can be used to predict the rolling resistances of other types of tyres.
The Impact of Forest Usage and Accessibility on the Perceptions of its Users and Surrounding Residents
Authors: Blaise Gloria C. , Brown Jeffrey A. , Jordan Rebecca C. , Sorensen Amanda E.
Published: December 2020

Forests provide valuable ecosystem services to individuals that live near them and visit them. However, many forests, especially in highly developed areas, are specifically managed for resource conservation purposes. A common practice for conservation is restricting access to people to ensure minimal human-driven harm. While the restriction of human access to a forest increases its biological ecosystem services, it limits its cultural services and may reduce the public’s perceived value of the forest. To investigate how access influences the perceived value of a forest, two forests in the state of New Jersey, USA, were compared. The forests, the Rutgers Ecological Preserve (RUEP) and Hutcheson Memorial Forest (HMF), represent accessible and inaccessible forests respectively. The study reported here evaluated the effects of visitation and accessibility on public perception of both sites. Residents near the RUEP and HMF, and visitors of both sites, were surveyed (n = 48). The results of the surveys demonstrated that the frequency of visitation and community location can have noticeable influences on a number of variables, including one’s belief that forests provide important services and the thought that protected areas take away from an individual’s freedom to use the area. A more positive view of the forests was present in those who visited the public forest most often.
Activation of Soluble Polysaccharides with 1-Cyano-4-Dimethylaminopyridine Tetrafluoroborate (CDAP) for Use in Protein–Polysaccharide Conjugate Vaccines and Immunological Reagents. III Optimization of CDAP Activation
Authors: Lees Andrew , Barr Jackson F. , Gebretnsae Samson
Published: December 2020

CDAP (1-cyano-4-dimethylaminopyridine tetrafluoroborate) is employed in the synthesis of conjugate vaccines as a cyanylating reagent. In the published method, which used pH 9 activation at 20 °C (Vaccine, 14:190, 1996), the rapid reaction made the process difficult to control. Here, we describe optimizing CDAP activation using dextran as a model polysaccharide. CDAP stability and reactivity were determined as a function of time, pH and temperature. While the rate of dextran activation was slower at lower pH and temperature, it was balanced by the increased stability of CDAP, which left more reagent available for reaction. Whereas maximal activation took less than 2.5 min at pH 9 and 20 °C, it took 10–15 min at 0 °C. At pH 7 and 0 °C, the optimal time increased to >3 h to achieve a high level of activation. Many buffers interfered with CDAP activation, but DMAP could be used to preadjust the pH of polysaccharide solutions so that the pH only needed to be maintained. We found that the stability of the activated dextran was relatively independent of pH over the range of pH 1–9, with the level of activation decreased by 40–60% over 2 h. The use of low temperature and a less basic pH, with an optimum reaction time, requires less CDAP, improving activation levels while making the process more reliable and easier to scale up.