bluuebunny/crossref_metadata_2025 huggingface dataset:
Cultivating Support for the Sustainable Development Goals, Green Strategy and Human Resource Management Practices in Future Business Leaders: The Role of Individual Differences and Academic Training
Authors: Westerman James W. , Nafees Lubna , Westerman Jennifer
Published: June 2021

How we effectively train our future business leaders is critical to the success of the implementation of the SDGs. Higher education will play a central role in this effort. This research examines business student support for environmentally oriented organizational strategy (“green strategy”) and human resource management policies and practices (“green hrm”) in comparison with sustainable development (SD) students to explore the barriers facing the education of our future business leaders on the SDGs. We explore whether student political orientation, gender, or authoritarianism are associated with different levels of support within each discipline. We also examine whether business students prioritize the same UN Sustainable Development Goals (SDGs) as SD students. Results (n = 281) indicate that business students (particularly those who are male, conservative, or authoritarian) are less supportive of green strategy and green HR than SD students. However, business student support of prosperity/people-oriented SDGs offers a potential avenue for progress.
Government Spending and Economic Growth: A Cointegration Analysis on Romania
Authors: Popescu Cristian C. , Diaconu (Maxim) Laura
Published: June 2021

The purpose of our study is to identify the nature of the link between government spending and economic growth, in order to test the two theories of Wagner and Keynes, in the case of Romania. On the one hand, Keynes argues that public spending is an important tool to stimulate growth. On the other hand, Wagner says that increased public spending is a result of economic growth. We analyzed the long-term dynamics of the two time series through Johansen’s cointegration approach and, in the short term, with the help of Granger’s causality test. The obtained results do not indicate the existence of long-term cointegration vectors, but they support the double causality relation in the short term. Therefore, not only does GDP represent a Granger cause for government spending but also vice versa. Our results validate the liberal criticism of the state’s involvement in supporting economies. As the critics of the monetarist school said, the effect of multiplying government spending on national income is short-term. The long-term effect appears under the action of inflationary macroeconomic bottlenecks.
Tourist Experience and Innovative Hospitality Management in Different Cities
Authors: Gelbman Alon
Published: June 2021

The complexity of modern tourism and hospitality management because of competition in the destination market, and especially in urban tourism destinations, has created a demand for creativity and innovation. To satisfy heightened tourist expectations for a specialized experience, hospitality organizations emphasize local culture characteristics and the urban community. The purpose of this paper is to examine how an urban hospitality organization emphasizes community and social values in its hostels, and how the tourist experience is adapted to each city’s culture and atmosphere (Nazareth, Jerusalem, and Tel Aviv). The theoretical framework is based on the link connecting urban hospitality with the tourist experience, and how tourism innovation and creativity is managed, during this age of competition and specialization. The qualitative methodology includes participant observation, document review, and in-depth interviews. The findings of this study add a new dimension to the existing knowledge, namely the role of creativity and innovation in helping the management of an urban hospitality organization to shape the tourist experience. The study developed a new unique model for “implementing innovation in urban hospitality management” which describes the framework of connections and interactions between the various sustainable community based and social aspects. The novelty of this research model lies in the emphasis on how management uses innovation and creativity to brand the whole chain so as to realize the vision and values it wishes to promote. This also entails a system of sub-positioning that aligns the vision and values with the distinctive culture of each city and with each local community’s nature and traditions.
Complex Thinking and Sustainable Social Development: Validity and Reliability of the COMPLEX-21 Scale
Authors: Tobón Sergio , Luna-Nemecio Josemanuel
Published: June 2021

Thinking skills are essential to achieve sustainable social development. Nonetheless, there is no specific instrument that assesses all of these skills as a whole. The present study aimed to design and validate a scale to assess complex thinking skills in adult people. A scale of 22 items assessing the following aspects: analysis and problem solving, critical analysis, metacognition, systemic analysis, and creativity, in five levels, was created. This scale was validated in 626 university students from Peru. In total, 16 experts in the field helped to determine the content validity of the scale (Aiken’s V value higher than 0.8). The confirmatory factor analysis allowed the evaluation of the structure of the five factors theoretically proposed and the goodness of fit indexes was satisfactory. An item was eliminated during the process and the scale resulted in 21 items. The composite reliability for the different factors was ranged between 0.794 and 0.867. The invariance between genders was also checked and the concurrent validity was proved. The study concludes that the content validity, construct validity, concurrent validity, and composite reliability levels of the COMPLEX-21 scale are appropriate.
Harmonization of Green Motives and Green Business Strategies towards Sustainable Development of Hospitality and Tourism Industry: Green Environmental Policies
Authors: Yousaf Zahid , Radulescu Magdalena , Sinisi Crenguta Ileana , Serbanescu Luminita , Paunescu Loredana Maria
Published: June 2021

This study aims to investigate the direct impact of green motives (GM) and green business strategies (GBS) on sustainable development (SD) in the hospitality sector. It explores the direct links between GM and SD. Moreover, the mediating role of GBS between GM and SD was tested. The research relies on the stakeholders’ theory, which states that the organization’s success and future development depends on the satisfaction of stakeholders. Data were collected from 451 top managers and owners from 54 hotels (5, 4 and 3-star hotels) operating in Pakistan. Quantitative analysis including correlation, regression, confirmatory factor analysis and structural equation model techniques were used. The mediating role of GBS was assessed using the bootstrapping method. Results proved that GM and GBS enable hotel industry to achieve the targets of SD. Finding also proved that GBS act as a mediator between the GM and SD link. The hotel industry needs attention to achieve the targets of SD and customers’ inclination towards more hygienic and environmental issues after the worldwide COVID-19 pandemic situation has forced the hotel industry to adapt GBS initiated through GM. The current research articulated this upcoming issue and offered a SD model for the hotel industry.
Main Design Considerations and Prospects of Contemporary Tall Timber Apartment Buildings: Views of Key Professionals from Finland
Authors: Karjalainen Markku , Ilgın Hüseyin Emre , Tulonen Lassi
Published: June 2021

As a result of increasing urbanization, the need for sustainable housing, e.g., tall (over eight-story) timber apartments, is increasing in Finland. Leveraging the experience of key Finnish professionals plays a significant role in the transition and expansion of sustainable timber housing as an essential part of the forest-based bioeconomy. This interview-based study will serve to fill a gap by examining the views of key professionals with experience in tall timber residential construction, using Finland as a case study. The 21 interviews primarily highlighted that: (1) the construction cost was the most important parameter affecting the architectural and structural design; (2) the most critical consideration influencing the overall design was reported to be the structural system selection and structural design, followed by city planning and client control; (3) key professionals assessed the building’s form and the main dimensions of the building’s mass as the most significant parameters affected by timber construction; (4) the main structural considerations that needed to be developed for tall timber housing were the bracing solutions and fittings, the structures of the intermediate floors, and load-bearing vertical/partition structures; (5) construction preconceptions, the lack of cost-competitiveness, and the lack of construction expertise/actors were considered to be major obstacles; (6) the most important parameters for the future of tall timber apartment buildings were reported to be education, timber construction marketing and public awareness, land delivery conditions, the unification of public administration, and financial support; (7) tall timber building design was reported to be a complex subject that requires close collaboration, especially between the architect, the structural designer and the wood supplier. This paper will help us to understand the boundary conditions affecting the design, the development needs in solutions, the importance of design parameters, the design parameters affected by timber construction, and the prospects, measures and obstacles to tall timber apartments from the perspectives of key Finnish professionals, thereby aiding the sound planning and development of tall timber housing projects.
Sustainable Streetscape and Built Environment Designs around BRT Stations: A Stated Choice Experiment Using 3D Visualizations
Authors: Adeel Ahmad , Notteboom Bruno , Yasar Ansar , Scheerlinck Kris , Stevens Jeroen
Published: June 2021

The incompatibility between the microscale-built environment designs around mass transit stations and stakeholders’ preferences causes dissatisfaction and inconvenience. The lack of a pedestrian-friendly environment, uncontrolled development patterns, traffic and parking issues make the street life vulnerable and unattractive for users, and affect the mass transit usage. How to design the streetscapes around mass transit stations to provide a user-friendly street environment is a crucial question to achieve sustainable transit-oriented development goals. To recognize the specific attributes of streetscape environment relevant in local context of BRT Lahore, this paper presents the results of a visual preference experiment in which nine attributes of built environment were systematically varied across choice sets. Multinomial logit models were set up to identify the preferences of three target groups: BRT users, commercial building users and residents at different locations. The research indicates that not only the road-related factors (bike lane and sidewalk widths, crossings facilities, street greenery) have a significant influence on people’s preference but also that building heights, and the typology of buildings and housing projects around BRT corridor have shaped these preferences. When planning and designing urban design projects around mass transit projects, these significant attributes should be considered.
Improving Irrigation Water Use Efficiency of Robusta Coffee (Coffea canephora) Production in Lam Dong Province, Vietnam
Authors: Tran Duyen Nhat Lam , Nguyen Tien Dinh , Pham Thuy Thu , Rañola Roberto F. , Nguyen Thinh An
Published: June 2021

Recent prolonged dry periods and lack of irrigation water have severely affected the productivity of coffee farms in the Central Highlands of Vietnam. This paper analyzes the efficiency of irrigation water use for Robusta coffee (Coffea canephora) in the Lam Dong province. A Cobb–Douglas production function was used to determine coffee productivity’s response to the application of irrigation water and other production factors using data collected from 194 farmers, while the technical efficiency (TE) and irrigation water use efficiency (IWUE) were analyzed using a data envelopment analysis (DEA) model. The correlation of different factors to IWUE was determined using the Tobit model. The production function analysis using Cobb–Douglas shows that the volume of irrigation water, amount of working capital, labor, and farm size significantly influence coffee productivity. Indigenous farmers are more efficient in utilizing irrigation water than migrant farmers. The Tobit result indicates that farmers’ experience, education level, the distance of farm to water sources, security of access to water sources, extension contact, and credit access significantly affect IWUE. The study findings further suggest that mitigating water shortages in coffee farms require subregional and national policy support such as better access to credit and extension services, training, land management, and household-level efforts to improve farming practices through the application of appropriate technologies and traditional knowledge.
Estimating the Economic Level of Water Losses (ELWL) in the Water Distribution System of the City of Malang, Indonesia
Authors: Heryanto Tri , Sharma Saroj K. , Daniel D. , Kennedy Maria
Published: June 2021

All of the possible strategies to reduce water losses in piped distribution systems follow the law of diminishing returns: the higher the expenditure on water loss reduction, the lower the progressive return in terms of water saved. Therefore, water utilities need to estimate the economic level of water losses (ELWL) so that they can reduce their water loss to the level where the cost to reduce the water losses is equal to the value of the water saved. This paper aims to estimate the ELWL using four different methods: the total cost method, the marginal cost method, the cumulative cost–benefit method, and the component-based methods. This analysis is based on data (2011–2016) on the water utilities of the city of Malang (PDAM Kota Malang), Indonesia. It was found that the total cost and marginal cost methods gave almost similar results for ELWL. However, the total cost method is preferred to calculate ELWL because it is the most accurate, easier to apply, and does not need a long data series. In addition, the estimated ELWL for PDAM Kota Malang was 21.76%, which is 3.71% higher than the water loss level estimated in 2016, which means that their strategies to reduce water loss are not cost-efficient. Moreover, the lack of data is a major challenge in the estimation of ELWL in Indonesia. This study emphasizes the importance of estimating the ELWL so that water utilities, especially in Indonesia, can evaluate their strategies in reducing water loss and improving their cost-effectiveness.
Geomorphological Processes at the Industrial Sludge Landfill in Sered, Slovakia
Authors: Michaeli Eva , Maxin Matúš , Solár Vladimír , Vilček Jozef , Boltižiar Martin
Published: June 2021

The principal aim of the study is to identify the nature and causes of changes to the surface of a landfill body of waste from nickel production located in the industrial zone of the town of Sereď (Slovak Republic). This change is related to natural and anthropogenic geomorphological processes characteristic of the temperate zone of the northern hemisphere. The landfill is an accumulative anthropogenic form of relief, and its body is composed from an artefact-metallurgical sludge, which has specific properties. The landfill constitutes a strange shape of relief at the Danubian Plane, which attracts attention with its physiognomy and also with the black color of the material significantly. It formed during the 30-year-long existence of the nickel smelter plant (1963–1993) and remained in this location, until the present day, for another 28 years after the end of production. Since 1994, the landfill has been the property of a private company that mines sludge in order to obtain residual metals.
Parts and Composites of Quantum Systems
Authors: Gudder Stanley P.
Published: June 2021

We consider three types of entities for quantum measurements. In order of generality, these types are observables, instruments and measurement models. If α and β are entities, we define what it means for α to be a part of β. This relationship is essentially equivalent to α being a function of β and in this case β can be employed to measure α. We then use the concept to define the coexistence of entities and study its properties. A crucial role is played by a map α^ which takes an entity of a certain type to one of a lower type. For example, if I is an instrument, then I^ is the unique observable measured by I. Composite systems are discussed next. These are constructed by taking the tensor product of the Hilbert spaces of the systems being combined. Composites of the three types of measurements and their parts are studied. Reductions in types to their local components are discussed. We also consider sequential products of measurements. Specific examples of Lüders, Kraus and trivial instruments are used to illustrate various concepts. We only consider finite-dimensional systems in this article. Finally, we mention the role of symmetry representations for groups using quantum channels.
The Association among Autistic Traits, Interactional Synchrony and Typical Pattern of Motor Planning and Execution in Neurotypical Individuals
Authors: Granner-Shuman Michal , Dahan Anat , Yozevitch Roi , Gvirts Problovski Hila Zahava
Published: June 2021

Autism spectrum disorder (ASD) is characterized by deficits in interactional synchrony and motor performance, but little is known about the association between them. The current study investigated the association among aberrant interactional synchrony (as measured by interactors’ symmetry in the form of the hand at each time-point along movement’s execution), motor functioning and the level of Autistic traits. In this study, autistic traits were evaluated by the Autistic Spectrum Quotient (AQ). Two tasks were used: (1) an interactional synchrony task where participants and the research assistant were instructed to move their hands together; and (2) a motor planning task which allows for continuous monitoring of natural hand movements. Pearson correlation analysis indicated a significant association between lower communication skills (i.e., higher AQ communication scores) and lower intentional synchrony rates. In addition, lower communication skills were found associated with typical patterns of motor planning and execution characterized by shorter time to start the movement and higher value of max speed. Mediator analyses supported the notion that aberrant intentional synchrony in individuals with low communication skills is partially mediated through typical patterns of motor planning and execution. These results suggest typical patterns of motor functions may account for intentional synchrony difficulties.
Graded Medial n-Ary Algebras and Polyadic Tensor Categories
Authors: Duplij Steven
Published: June 2021

Algebraic structures in which the property of commutativity is substituted by the mediality property are introduced. We consider (associative) graded algebras and instead of almost commutativity (generalized commutativity or ε-commutativity), we introduce almost mediality (“commutativity-to-mediality” ansatz). Higher graded twisted products and “deforming” brackets (being the medial analog of Lie brackets) are defined. Toyoda’s theorem which connects (universal) medial algebras with abelian algebras is proven for the almost medial graded algebras introduced here. In a similar way we generalize tensor categories and braided tensor categories. A polyadic (non-strict) tensor category has an n-ary tensor product as an additional multiplication with n−1 associators of the arity 2n−1 satisfying a n2+1-gon relation, which is a polyadic analog of the pentagon axiom. Polyadic monoidal categories may contain several unit objects, and it is also possible that all objects are units. A new kind of polyadic categories (called groupal) is defined: they are close to monoidal categories but may not contain units: instead the querfunctor and (natural) functorial isomorphisms, the quertors, are considered (by analogy with the querelements in n-ary groups). The arity-nonreducible n-ary braiding is introduced and the equation for it is derived, which for n=2 coincides with the Yang–Baxter equation. Then, analogously to the first part of the paper, we introduce “medialing” instead of braiding and construct “medialed” polyadic tensor categories.
Asymmetry Evolvement and Controllability of a Symmetric Hyperchaotic Map
Authors: Kong Sixiao , Li Chunbiao , Jiang Haibo , Zhao Yibo , Wang Yanling
Published: June 2021

Trigonometric functions were used to construct a 2-D symmetrical hyperchaotic map with infinitely many attractors. The regime of multistability depends on the periodicity of the trigonometric function, which is closely related to the initial condition. For this trigonometric nonlinearity and the introduction of an offset controller, the initial condition triggers a specific multistability evolvement, in which infinitely countless symmetric and asymmetric attractors are produced. Initial condition-triggered offset boosting is explored, combined with constant controlled offset regulation. Furthermore, this symmetric map gives the sequences in various types of asymmetric attractors, in which the polarity balance is maintained by the initial condition and a negative coefficient due to the trigonometric function. Finally, as determined through the hardware implementation of STM32, the corresponding results agree with the numerical simulation.
Numerical Analysis of the Flow Effect of the Menger-Type Artificial Reefs with Different Void Space Complexity Indices
Authors: Wang Xinxin , Liu Xianyi , Tang Yanli , Zhao Fenfang , Luo Yan
Published: June 2021

Based on fractal theory, a regular fractal is used to construct symmetrical reef models (e.g., cube and triangle reef models) with different fractal levels (n = 1, 2, 3). Using the concept of fractal dimension, we can better understand the spatial effectiveness of artificial reefs. The void space complexity index is defined to quantify the complexity of the internal spatial distribution of artificial reefs models under different levels. The computational fluid dynamics (CFD) flow simulation approach was used to investigate the effects of void space complexity on the flow field performances of the symmetrical artificial reef models. The upwelling convection index (Hupwelling/HAR, Vupwelling/VAR), wake recirculating index (Lwake/LAR, Vwake/VAR) and non-dimensionalized velocity ratio range were used to evaluate the efficiency of the flow field effect inside or around artificial reefs. The surface area and spatial complexity index of artificial reefs increase with increasing fractal level. The numerical simulation data shows that the Menger-type artificial reef models with a higher spatial complexity index have better flow field performances in the upwelling and wake regions. Compared to the traditional artificial reef models, the upwelling convection index (Vupwelling/VAR) and recirculating index (Vwake/VAR) of n = 3 fractal cube artificial reef increase by 37.5% and 46.8%, respectively. The efficiency indices of the upwelling region and wake region around the fractal triangle artificial reef model are 2–3 times those of the fractal cube artificial reef model when the fractal level is 3.
Symmetric and Asymmetric Data in Solution Models
Authors: Zavadskas Edmundas Kazimieras , Antucheviciene Jurgita , Turskis Zenonas
Published: June 2021

This Special Issue covers symmetric and asymmetric data that occur in real-life problems. We invited authors to submit their theoretical or experimental research to present engineering and economic problem solution models that deal with symmetry or asymmetry of different data types. The Special Issue gained interest in the research community and received many submissions. After rigorous scientific evaluation by editors and reviewers, seventeen papers were accepted and published. The authors proposed different solution models, mainly covering uncertain data in multi-criteria decision-making problems as complex tools to balance the symmetry between goals, risks, and constraints to cope with the complicated problems in engineering or management. Therefore, we invite researchers interested in the topics to read the papers provided in the Special Issue.
The Presence of Mycotoxins in Human Amniotic Fluid
Authors: Gromadzka Karolina , Pankiewicz Jakub , Beszterda Monika , Paczkowska Magdalena , Nowakowska Beata , Kocyłowski Rafał
Published: June 2021

Mycotoxin exposure assessments through biomonitoring studies, based on the analysis of amniotic fluid, provides useful information about potential exposure of mothers and fetuses to ubiquitous toxic metabolites that are routinely found in food and the environment. In this study, amniotic fluid samples (n = 86) were collected via abdominal amniocentesis at 15–22 weeks of gestation from pregnant women with a high risk of chromosomal anomalies or genetic fetal defects detected during 1st trimester prenatal screening. These samples were analyzed for the presence of the most typical Aspergillus, Penicillium and Fusarium mycotoxins, with a focus on aflatoxins, ochratoxins and trichothecenes, using the LC-FLD/DAD method. The results showed that the toxin was present in over 75% of all the tested samples and in 73% of amniotic fluid samples from fetuses with genetic defects. The most frequently identified toxins were nivalenol (33.7%) ranging from <LOQ to 4037.6 ng/mL, and aflatoxins (31.4%), including aflatoxin G1, ranging from <LOQ to 0.4 ng/mL. Ochratoxin A and deoxynivalenol were identified in 26.7% and 27.9% of samples, respectively. Bearing in mind the above, the detection of mycotoxin levels in amniotic fluid is useful for the estimation of overall risk characterization with an attempt to link the occurrence of fetal abnormalities with exposure to mycotoxins in utero.
Applications of pT-xR Variables in Describing Inclusive Cross Sections at the LHC
Authors: Taylor Frank E.
Published: June 2021

Invariant inclusive single-particle/jet cross sections in p–p collisions can be factorized in terms of two separable pT dependences, a [pT−s] sector and an [xR−pT−s] sector. Here, we extend our earlier work by analyzing more extensive data to explore various s-dependent attributes and other systematics of inclusive jet, photon and single particle reactions. Approximate power laws in s, pT and xR are found. Physical arguments are given which relate observations to the underlying physics of parton–parton hard scattering and the parton distribution functions in the proton. We show that the A(s,pT) function, introduced in our earlier publication to describe the pT dependence of the inclusive cross section, is directly related to the underlying hard parton–parton scattering for jet production, with little influence from soft physics. In addition to the a function, we introduce another function, the F(s,xR) function that obeys radial scaling for inclusive jets and offers another test of the underlying parton physics. An application to heavy ion physics is given, where we use our variables to determine the transparency of cold nuclear matter to penetrating heavy mesons through the lead nucleus.
Specificity of the HIV-1 Protease on Substrates Representing the Cleavage Site in the Proximal Zinc-Finger of HIV-1 Nucleocapsid Protein
Authors: Mótyán János András , Miczi Márió , Oroszlan Stephen , Tőzsér József
Published: June 2021

To explore the sequence context-dependent nature of the human immunodeficiency virus type 1 (HIV-1) protease’s specificity and to provide a rationale for viral mutagenesis to study the potential role of the nucleocapsid (NC) processing in HIV-1 replication, synthetic oligopeptide substrates representing the wild-type and modified versions of the proximal cleavage site of HIV-1 NC were assayed as substrates of the HIV-1 protease (PR). The S1′ substrate binding site of HIV-1 PR was studied by an in vitro assay using KIVKCF↓NCGK decapeptides having amino acid substitutions of N17 residue of the cleavage site of the first zinc-finger domain, and in silico calculations were also performed to investigate amino acid preferences of S1′ site. Second site substitutions have also been designed to produce “revertant” substrates and convert a non-hydrolysable sequence (having glycine in place of N17) to a substrate. The specificity constants obtained for peptides containing non-charged P1′ substitutions correlated well with the residue volume, while the correlation with the calculated interaction energies showed the importance of hydrophobicity: interaction energies with polar residues were related to substantially lower specificity constants. Cleavable “revertants” showed one residue shift of cleavage position due to an alternative productive binding mode, and surprisingly, a double cleavage of a substrate was also observed. The results revealed the importance of alternative binding possibilities of substrates into the HIV-1 PR. The introduction of the “revertant” mutations into infectious virus clones may provide further insights into the potential role of NC processing in the early phase of the viral life-cycle.
Use of a Specific Phage Cocktail for Soft Rot Control on Ware Potatoes: A Case Study
Authors: Bugaeva Eugenia N. , Voronina Maya V. , Vasiliev Dmitry M. , Lukianova Anna A. , Landyshev Nikolay N. , Ignatov Alexander N. , Miroshnikov Konstantin A.
Published: June 2021

Using bacteriophages (bacterial viruses) to control pathogenic bacteria is a promising approach in horticulture. However, the application of this strategy in real conditions requires compliance with particular technological and environmental restraints. The presented paper concerns the process of phage selection to create a cocktail that is efficient against the circulating causal agents of potato soft rot. The resulting phage cocktail causes a complete lysis of a mixture of circulating pectobacterial strains in vitro. In the context of being used to treat ware potatoes during off-season storage, the protocol of phage application via the humidity maintenance system was designed. The phage cocktail was shown to reduce the population of Pectobacterium spp. 10–12-fold, achieving a population that was below a symptomatic threshold.
A Comprehensive Molecular Epidemiological Analysis of SARS-CoV-2 Infection in Cyprus from April 2020 to January 2021: Evidence of a Highly Polyphyletic and Evolving Epidemic
Authors: Chrysostomou Andreas C. , Vrancken Bram , Koumbaris George , Themistokleous George , Aristokleous Antonia , Masia Christina , Eleftheriou Christina , Iοannou Costakis , Stylianou Dora C. , Ioannides Marios , Petrou Panagiotis , Georgiou Vasilis , Hatziyianni Amalia , Lemey Philippe , Vandamme Anne-Mieke , Patsalis Philippos P. , Kostrikis Leondios G.
Published: June 2021

The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) resulted in an extraordinary global public health crisis. In early 2020, Cyprus, among other European countries, was affected by the SARS-CoV-2 epidemic and adopted lockdown measures in March 2020 to limit the initial outbreak on the island. In this study, we performed a comprehensive retrospective molecular epidemiological analysis (genetic, phylogenetic, phylodynamic and phylogeographic analyses) of SARS-CoV-2 isolates in Cyprus from April 2020 to January 2021, covering the first ten months of the SARS-CoV-2 infection epidemic on the island. The primary aim of this study was to assess the transmissibility of SARS-CoV-2 lineages in Cyprus. Whole SARS-CoV-2 genomic sequences were generated from 596 clinical samples (nasopharyngeal swabs) obtained from community-based diagnostic testing centers and hospitalized patients. The phylogenetic analyses revealed a total of 34 different lineages in Cyprus, with B.1.258, B.1.1.29, B.1.177, B.1.2, B.1 and B.1.1.7 (designated a Variant of Concern 202012/01, VOC) being the most prevalent lineages on the island during the study period. Phylodynamic analysis showed a highly dynamic epidemic of SARS-CoV-2 infection, with three consecutive surges characterized by specific lineages (B.1.1.29 from April to June 2020; B.1.258 from September 2020 to January 2021; and B.1.1.7 from December 2020 to January 2021). Genetic analysis of whole SARS-CoV-2 genomic sequences of the aforementioned lineages revealed the presence of mutations within the S protein (L18F, ΔH69/V70, S898F, ΔY144, S162G, A222V, N439K, N501Y, A570D, D614G, P681H, S982A and D1118H) that confer higher transmissibility and/or antibody escape (immune evasion) upon the virus. Phylogeographic analysis indicated that the majority of imports and exports were to and from the United Kingdom (UK), although many other regions/countries were identified (southeastern Asia, southern Europe, eastern Europe, Germany, Italy, Brazil, Chile, the USA, Denmark, the Czech Republic, Slovenia, Finland, Switzerland and Pakistan). Taken together, these findings demonstrate that the SARS-CoV-2 infection epidemic in Cyprus is being maintained by a continuous influx of lineages from many countries, resulting in the establishment of an ever-evolving and polyphyletic virus on the island.
HERC5 and the ISGylation Pathway: Critical Modulators of the Antiviral Immune Response
Authors: Mathieu Nicholas A. , Paparisto Ermela , Barr Stephen D. , Spratt Donald E.
Published: June 2021

Mammalian cells have developed an elaborate network of immunoproteins that serve to identify and combat viral pathogens. Interferon-stimulated gene 15 (ISG15) is a 15.2 kDa tandem ubiquitin-like protein (UBL) that is used by specific E1–E2–E3 ubiquitin cascade enzymes to interfere with the activity of viral proteins. Recent biochemical studies have demonstrated how the E3 ligase HECT and RCC1-containing protein 5 (HERC5) regulates ISG15 signaling in response to hepatitis C (HCV), influenza-A (IAV), human immunodeficiency virus (HIV), SARS-CoV-2 and other viral infections. Taken together, the potent antiviral activity displayed by HERC5 and ISG15 make them promising drug targets for the development of novel antiviral therapeutics that can augment the host antiviral response. In this review, we examine the emerging role of ISG15 in antiviral immunity with a particular focus on how HERC5 orchestrates the specific and timely ISGylation of viral proteins in response to infection.
Impact of Prior Influenza and Pneumoccocal Vaccines on Humoral and Cellular Response to SARS-CoV-2 BNT162b2 Vaccination
Authors: Puro Vincenzo , Castilletti Concetta , Agrati Chiara , Goletti Delia , Leone Sara , Agresta Alessandro , Cimini Eleonora , Tartaglia Eleonora , Casetti Rita , Colavita Francesca , Meschi Silvia , Matusali Giulia , Lapa Daniele , Najafi Fard Saeid , Aiello Alessandra , Farroni Chiara , Gallì Paola , Capobianchi Maria , Ippolito Giuseppe ,   on behalf of the INMI COVID-19 Vaccine Study Group
Published: June 2021

Vaccination against SARS-CoV-2 is considered the most effective method of prevention to contain the pandemic. While highly effective SARS-CoV-2 vaccines are being applied on a large-scale, whether and to what extent the strength of the vaccine-induced immune response could be further potentiated is still an object of debate. Several reports studied the effect of different vaccines on the susceptibility and mortality of COVID-19, with conflicting results. We aimed to evaluate whether previous influenza and/or pneumococcal vaccination had an impact on the specific immune response to the SARS-CoV-2 BNT162b2 mRNA vaccine. The study population consists of 710 workers from our Institute who completed the BNT162b2 schedule and have been tested at least once after the second dose, from 27 December 2020 up to 15 April 2021. Of these, 152 (21.4%) had received an influenza and 215 (30.3%) a concomitant influenza and pneumococcal vaccination, a median of 102 days before the second dose of BNT162b2. Overall, 100% of workers were tested for anti-Spike receptor-binding domain (anti-S/RBD) antibodies, 224 workers for neutralization titer (Micro-neutralization assay, MNA), and 155 workers for a spike-specific T cell interferon-γ response (IFN-γ). The levels of anti-S/RBD, MNA and IFN-γ were evaluated and compared according to sex, age, involvement in direct care of COVID-19 patients, and previous influenza/pneumococcal vaccination. At the univariate analysis, no statistically significant association was observed with regard to a previous influenza and pneumococcal vaccination. A significant lower anti-S/RBD response was observed according to an older age and male sex, while MNA titers were significantly associated to sex but not to age. At the multivariable analysis, workers receiving a concomitant influenza and pneumococcal vaccination or only influenza showed a 58% (p 0.01) and 42% (p 0.07) increase in MNA titers, respectively, compared to those who did not receive an influenza/pneumococcal vaccination. Female workers showed an 81% MNA and a 44% anti-S/RBD increase compared to male workers (p < 0.001). Compared to workers aged 21 to 49 years, those aged 50 or older were associated with a reduction in the anti-S/RBD (16%; p 0.005), MNA (31%; p 0.019), and IFN.g (32%) immune response. Maintaining the influenza and pneumococcal immunization program for the coming season, in which COVID-19 could still be spreading, remains strongly recommended to protect those who are more vulnerable and to limit the potential burden of these infections on the healthcare system.
Global Vaccine Hesitancy Segmentation: A Cross-European Approach
Authors: Recio-Román Almudena , Recio-Menéndez Manuel , Román-González María Victoria
Published: June 2021

Vaccine-preventable diseases are global mainly in a globalized world that is characterized by a continuous movement of people and goods across countries. Vaccine hesitancy, the reluctance or refusal to vaccinate despite the availability of vaccines, is rising worldwide. What if the problem of vaccine hesitancy could be most effectively managed when treated globally rather than on a national or regional basis? What if a global vaccine-hesitant segment exists and the differences among countries are not so significant? Based on the Global Marketing Strategy paradigm, this paper shows that seven different cross-European segments exist based on the beliefs, attitudes, and behaviors collected in 28 European countries. These pan-European segments are differentiable (people in those segments have similar characteristics that are visibly dissimilar from the ones in other segments) and actionable (organizations would be able to propose interventions to the hesitant segments based on their profiles). With segmentation being the starting point of many public health intervention strategies for avoiding vaccine-hesitancy, the results recommend moderating the full-adaptation strategy that follows the “context matters” principle suggested by several political and public health international organizations. Embracing a more standardized strategy will allow the development of better services and strategies that support and enable desirable vaccination behaviors.
Novel Capsid-Specific Single-Domain Antibodies with Broad Foot-and-Mouth Disease Strain Recognition Reveal Differences in Antigenicity of Virions, Empty Capsids, and Virus-Like Particles
Authors: Li Haozhou , Dekker Aldo , Sun Shiqi , Burman Alison , Kortekaas Jeroen , Harmsen Michiel M.
Published: June 2021

Foot-and-mouth disease (FMD) vaccine efficacy is mainly determined by the content of intact virions (146S) and empty capsids (75S). Both particles may dissociate into 12S subunits upon vaccine manufacturing, formulation, and storage, reducing vaccine potency. We report the isolation of capsid-specific llama single-domain antibodies (VHHs) with broad strain recognition that can be used to quantify intact capsids in FMD vaccines by double antibody sandwich (DAS) ELISA. One capsid-specific VHH displayed remarkably broad strain reactivity, recognizing 14 strains representing the 13 most important lineages of serotype A, and two VHHs cross-reacted with other serotypes. We additionally show that the newly isolated VHHs, as well as previously characterized VHHs, can be used to identify antigenic differences between authentic 146S and 75S capsids, as well as corresponding genetically engineered virus-like particles (VLPs). Our work underscores that VHHs are excellent tools for monitoring the quantity and stability of intact capsids during vaccine manufacturing, formulation, and storage, and additionally shows that VHHs can be used to predict the native-like structure of VLPs.
Vaccination in PADs
Authors: Milito Cinzia , Soccodato Valentina , Collalti Giulia , Lanciarotta Alison , Bertozzi Ilaria , Rattazzi Marcello , Scarpa Riccardo , Cinetto Francesco
Published: June 2021

Primary antibody deficiencies (PADs) are the most common primary immunodeficiencies (PIDs). They can be divided into the following groups, depending on their immunological features: agammaglobulinemia; common variable immunodeficiency (CVID) isotype; hyper IgM isotype; light chain or functional deficiencies with normal B cell count; specific antibody deficiency with normal Ig concentrations and normal numbers of B cells and transient hypogammaglobulinemia of infancy. The role of vaccination in PADs is recognized as therapeutic, diagnostic and prognostic and may be used in patients with residual B-cell function to provide humoral immunity to specific infective agents. According to their content and mechanisms, vaccines are grouped as live attenuated, inactivated (conjugated, polysaccharide), mRNA or replication-deficient vector vaccines. Vaccination may be unsafe or less effective when using certain vaccines and in specific types of immunodeficiency. Inactivated vaccines can be administered in PAD patients even if they could not generate a protective response; live attenuated vaccines are not recommended in major antibody deficiencies. From December 2020, European Medicines Agency (EMA) approved vaccines against COVID-19 infection: according to ESID advises, those vaccinations are recommended in patients with PADs. No specific data are available on safety and efficacy in PAD patients.
Seroprevalence of SARS-CoV-2 Antibodies in Symptomatic Individuals Is Higher than in Persons Who Are at Increased Risk Exposure: The Results of the Single-Center, Prospective, Cross-Sectional Study
Authors: Zurochka Alexandr , Dobrinina Maria , Zurochka Vladimir , Hu Desheng , Solovyev Alexandr , Ryabova Liana , Kritsky Igor , Ibragimov Roman , Sarapultsev Alexey
Published: June 2021

The present study aimed to assess antibody seropositivity prevalence among symptomatic individuals and individuals with a high risk of occupational exposure to SARS-CoV-2. Participants from Chelyabinsk (Russian Federation) who were at an increased risk of exposure to SARS-CoV-2 (high-risk group, n = 1091) and participants who either had symptoms consistent with COVID-19 or were suspected to have experienced COVID-19 in the past (symptomatic group, n = 692) were enrolled between 28 September and 30 December 2020. Blood samples were tested by enzyme-linked immunosorbent assay D-5501 SARS-Cov-2-IgG-EIA-BEST and D-5502 SARS-Cov-2-IgM-EIA-BEST (AO Vector-Best, Novosibirsk, Russia). The overall seropositivity rate was 28.33–28.53%. SARS-CoV-2 antibodies were detected in 17.23% (adjusted prevalence of 17.17–17.29%) of participants in the high-risk and 45.95% (adjusted prevalence of 45.91–46.24%) in the symptomatic group. Higher IgG and IgM titers were observed in women compared to men, as well as in participants in the symptomatic group compared to those in the high-risk group. The results indicate that the seroprevalence among residents in several Russian regions is low (28.38%) and inadequate to provide herd immunity. The lower seroprevalence among participants in the high-risk group may be attributed to the enforcement of healthcare protocols and the use of adequate personal protective equipment.
CO2 and CH4 Emissions from an Arid Fluvial Network on the Chinese Loess Plateau
Authors: Chan Chun-Ngai , Shi Hongyan , Liu Boyi , Ran Lishan
Published: June 2021

The emissions of greenhouse gases (GHGs) from inland waters are an important component of the global carbon (C) cycle. However, the current understanding of GHGs emissions from arid river systems remains largely unknown. To shed light on GHGs emissions from inland waters in arid regions, high-resolution carbon dioxide (CO2) and methane (CH4) emission measurements were carried out in the arid Kuye River Basin (KRB) on the Chinese Loess Plateau to examine their spatio-temporal variability. Our results show that all streams and rivers were net C sources, but some of the reservoirs in the KRB became carbon sinks at certain times. The CO2 flux (FCO2) recorded in the rivers (91.0 mmol m−2 d−1) was higher than that of the reservoirs (10.0 mmol m−2 d−1), while CH4 flux (FCH4) in rivers (0.35 mmol m−2 d−1) was lower than that of the reservoirs (0.78 mmol m−2 d−1). The best model developed from a number of environmental parameters was able to explain almost 40% of the variability in partial pressure of CO2 (pCO2) for rivers and reservoirs, respectively. For CH4 emissions, at least 70% of the flux occurred in the form of ebullition. The emissions of CH4 in summer were more than threefold higher than in spring and autumn, with water temperature being the key environmental variable affecting emission rates. Since the construction of reservoirs can alter the morphology of existing fluvial systems and consequently the characteristics of CO2 and CH4 emissions, we conclude that future sampling efforts conducted at the basin scale need to cover both rivers and reservoirs concurrently.
Nearshore Fish Species Richness and Species–Habitat Associations in the St. Clair–Detroit River System
Authors: Hilling Corbin D. , Fischer Jason L. , Ross Jason , Tucker Taaja R. , DeBruyne Robin L. , Mayer Christine M. , Roseman Edward F.
Published: June 2021

Shallow water riparian zones of large rivers provide important habitat for fishes, but anthropogenic influences have reduced the availability and quality of these habitats. In the St. Clair–Detroit River System, a Laurentian Great Lakes connecting channel, losses of riparian habitat contributed to impairment of fish populations and their habitats. We conducted a seine survey annually from 2013 to 2019 at ten sites in the St. Clair and Detroit rivers to assess riparian fish communities, and to identify habitat attributes associated with fish species richness and catches of common species. We captured a total of 38,451 fish representing 60 species, with emerald shiner Notropis atherinoides composing the largest portion of the catch. We used an information-theoretic approach to assess the associations between species richness and catches of 33 species with habitat variables (substrate, shoreline vegetation types, and aquatic macrophyte richness). Sand, cobble, and algal substrates and shoreline vegetation were important predictors of species richness based on a multimodel inference approach. However, habitat associations of individual species varied. This work identified manageable habitat variables associated with species richness, while identifying potential tradeoffs for individual species. Further, this work provides baselines for development and evaluation of fish community and shoreline habitat restoration goals.