bluuebunny/crossref_metadata_2025 huggingface dataset:
Learning from Projection to Reconstruction: A Deep Learning Reconstruction Framework for Sparse-View Phase Contrast Computed Tomography via Dual-Domain Enhancement
Authors: Zhang Changsheng , Fu Jian , Zhao Gang
Published: May 2023

Phase contrast computed tomography (PCCT) provides an effective non-destructive testing tool for weak absorption objects. Limited by the phase stepping principle and radiation dose requirement, sparse-view sampling is usually performed in PCCT, introducing severe artifacts in reconstruction. In this paper, we report a dual-domain (i.e., the projection sinogram domain and image domain) enhancement framework based on deep learning (DL) for PCCT with sparse-view projections. It consists of two convolutional neural networks (CNN) in dual domains and the phase contrast Radon inversion layer (PCRIL) to connect them. PCRIL can achieve PCCT reconstruction, and it allows the gradients to backpropagate from the image domain to the projection sinogram domain while training. Therefore, parameters of CNNs in dual domains are updated simultaneously. It could overcome the limitations that the enhancement in the image domain causes blurred images and the enhancement in the projection sinogram domain introduces unpredictable artifacts. Considering the grating-based PCCT as an example, the proposed framework is validated and demonstrated with experiments of the simulated datasets and experimental datasets. This work can generate high-quality PCCT images with given incomplete projections and has the potential to push the applications of PCCT techniques in the field of composite imaging and biomedical imaging.
Special Issue on Role of Microbes in Agriculture and Food
Authors: Kordowska-Wiater Monika , Pytka Monika
Published: May 2023

Microorganisms play an important role in human life by influencing agricultural crops, which are raw materials used in the production of food [...]
Evaluation of Accuracy Degradation Resulting from Concept Drift in a Fake News Detection System Using Emotional Expression
Authors: Murayama Hirokazu , Suzuki Kaiyu , Matsuzawa Tomofumi
Published: May 2023

Fake news on social media has become a social problem. Fake news refers to false information that is deliberately intended to deceive people. Several studies have been conducted on automatic detection systems that reduce the damage caused by fake news. However, most studies address the improvements made in detection accuracy, and real-world operations are rarely discussed. As the contents and expressions of fake news change over time, a model with a high detection accuracy loses accuracy after a few years. This phenomenon is called concept drift. As most conventional methods employ word representations, these methods exhibit accuracy degradation resulting from changes in word fads and usage. However, methods using the sentiment information of words can identify inflammatory sentences, which is a characteristic of fake news, and may suppress performance degradation caused by concept drift. In this study, a model using vector representations obtained from an emotion dictionary was compared with a model using conventional word embedding. Subsequently, we verified the resistance of the model to performance degradation. The results revealed the method using sentiment representation is less susceptible to concept drift. Models and learning methods that can achieve both detection accuracy and resistance to accuracy degradation can enable further development of fake news detection systems.
Morphology and Ultrastructure of the Female Reproductive Apparatus of an Asexual Strain of the Endoparasitoid Meteorus pulchricornis (Wesmael) (Hymenoptera, Braconidae)
Authors: Chen Yusi , Wang Pengzhan , Shu Xiaohan , Wang Zhizhi , Chen Xuexin
Published: May 2023

Meteorus pulchricornis (Wesmael) is a solitary endoparasitoid of lepidopteran pests and a good candidate for the control of Spodoptera frugiperda. To elucidate the structure of the female reproductive apparatus, which may play a role in facilitating successful parasitism, we presented the description of the morphology and ultrastructure of the whole female reproductive system in a thelytokous strain of M. pulchricornis. Its reproductive system includes a pair of ovaries without specialized ovarian tissues, a branched venom gland, a venom reservoir, and a single Dufour gland. Each ovariole contains follicles and oocytes at different stages of maturation. A fibrous layer, possibly an egg surface protector, coats the surface of mature eggs. The venom gland consists of secretory units (including secretory cells and ducts) with abundant mitochondria, vesicles and end apparatuses in the cytoplasm, and a lumen. The venom reservoir is comprised of a muscular sheath, epidermal cells with few end apparatuses and mitochondria, and a large lumen. Furthermore, venosomes are produced by secretory cells and delivered into the lumen via the ducts. As a result, myriad venosomes are observed in the venom gland filaments and the venom reservoir, suggesting that they may function as a parasitic factor and have important roles in effective parasitism.
Advanced Proteomic and Bioinformatic Tools for Predictive Analysis of Allergens in Novel Foods
Authors: López-Pedrouso María , Lorenzo José M. , Alché Juan de Dios , Moreira Ramón , Franco Daniel
Published: May 2023

In recent years, novel food is becoming an emerging trend increasingly more demanding in developed countries. Food proteins from vegetables (pulses, legumes, cereals), fungi, bacteria and insects are being researched to introduce them in meat alternatives, beverages, baked products and others. One of the most complex challenges for introducing novel foods on the market is to ensure food safety. New alimentary scenarios drive the detection of novel allergens that need to be identified and quantified with the aim of appropriate labelling. Allergenic reactions are mostly caused by proteins of great abundance in foods, most frequently of small molecular mass, glycosylated, water-soluble and with high stability to proteolysis. The most relevant plant and animal food allergens, such as lipid transfer proteins, profilins, seed storage proteins, lactoglobulins, caseins, tropomyosins and parvalbumins from fruits, vegetables, nuts, milk, eggs, shellfish and fish, have been investigated. New methods for massive screening in search of potential allergens must be developed, particularly concerning protein databases and other online tools. Moreover, several bioinformatic tools based on sequence alignment, motif identification or 3-D structure predictions should be implemented as well. Finally, targeted proteomics will become a powerful technology for the quantification of these hazardous proteins. The ultimate objective is to build an effective and resilient surveillance network with this cutting-edge technology.
Activation, Amplification, and Ablation as Dynamic Mechanisms of Dendritic Cell Maturation
Authors: Bourque Jessica , Hawiger Daniel
Published: May 2023

T cell responses to cognate antigens crucially depend on the specific functionality of dendritic cells (DCs) activated in a process referred to as maturation. Maturation was initially described as alterations of the functional status of DCs in direct response to multiple extrinsic innate signals derived from foreign organisms. More recent studies, conducted mainly in mice, revealed an intricate network of intrinsic signals dependent on cytokines and various immunomodulatory pathways facilitating communication between individual DCs and other cells for the orchestration of specific maturation outcomes. These signals selectively amplify the initial activation of DCs mediated by innate factors and dynamically shape DC functionalities by ablating DCs with specific functions. Here, we discuss the effects of the initial activation of DCs that crucially includes the production of cytokine intermediaries to collectively achieve amplification of the maturation process and further precise sculpting of the functional landscapes among DCs. By emphasizing the interconnectedness of the intracellular and intercellular mechanisms, we reveal activation, amplification, and ablation as the mechanistically integrated components of the DC maturation process.
Lipoxin A4 (LXA4) Reduces Alkali-Induced Corneal Inflammation and Neovascularization and Upregulates a Repair Transcriptome
Authors: He Jiucheng , Pham Thang L. , Kakazu Azucena H. , Ponnath Abhilash , Do Khanh V. , Bazan Haydee E. P.
Published: May 2023

Purpose: To investigate the anti-inflammatory and anti-angiogenic effects of the bioactive lipid mediator LXA4 on a rat model of severe corneal alkali injury. Methods: To induce a corneal alkali injury in the right eyes of anesthetized Sprague Dawley rats. They were injured with a Φ 4 mm filter paper disc soaked in 1 N NaOH placed on the center of the cornea. After injury, the rats were treated topically with LXA4 (65 ng/20 μL) or vehicle three times a day for 14 days. Corneal opacity, neovascularization (NV), and hyphema were recorded and evaluated in a blind manner. Pro-inflammatory cytokine expression and genes involved in cornel repair were assayed by RNA sequencing and capillary Western blot. Cornea cell infiltration and monocytes isolated from the blood were analyzed by immunofluorescence and by flow cytometry. Results: Topical treatment with LXA4 for two weeks significantly reduced corneal opacity, NV, and hyphema compared to the vehicle treatment. RNA-seq and Western blot results showed that LXA4 decreased the gene and protein expression of pro-inflammatory cytokines interleukin (IL)-1β and IL-6 and pro-angiogenic mediators matrix metalloproteinase (MMP)-9 and vascular endothelial growth factor (VEGFA). It also induces genes involved in keratinization and ErbB signaling and downregulates immune pathways to stimulate wound healing. Flow cytometry and immunohistochemistry showed significantly less infiltration of neutrophils in the corneas treated with LXA4 compared to vehicle treatment. It also revealed that LXA4 treatment increases the proportion of type 2 macrophages (M2) compared to M1 in blood-isolated monocytes. Conclusions: LXA4 decreases corneal inflammation and NV induced by a strong alkali burn. Its mechanism of action includes inhibition of inflammatory leukocyte infiltration, reduction in cytokine release, suppression of angiogenic factors, and promotion of corneal repair gene expression and macrophage polarization in blood from alkali burn corneas. LXA4 has potential as a therapeutic candidate for severe corneal chemical injuries.
Motif2Mol: Prediction of New Active Compounds Based on Sequence Motifs of Ligand Binding Sites in Proteins Using a Biochemical Language Model
Authors: Yoshimori Atsushi , Bajorath Jürgen
Published: May 2023

In drug design, the prediction of new active compounds from protein sequence data has only been attempted in a few studies thus far. This prediction task is principally challenging because global protein sequence similarity has strong evolutional and structural implications, but is often only vaguely related to ligand binding. Deep language models adapted from natural language processing offer new opportunities to attempt such predictions via machine translation by directly relating amino acid sequences and chemical structures to each based on textual molecular representations. Herein, we introduce a biochemical language model with transformer architecture for the prediction of new active compounds from sequence motifs of ligand binding sites. In a proof-of-concept application on inhibitors of more than 200 human kinases, the Motif2Mol model revealed promising learning characteristics and an unprecedented ability to consistently reproduce known inhibitors of different kinases.
Inhibition of NADPH Oxidase (NOX) 2 Mitigates Colitis in Mice with Impaired Macrophage AMPK Function
Authors: Banskota Suhrid , Wang Huaqing , Kwon Yun Han , Gautam Jaya , Haq Sabah , Grondin Jensine , Steinberg Gregory R. , Khan Waliul I.
Published: May 2023

Macrophage adenosine monophosphate-activated protein kinase (AMPK) limits the development of experimental colitis. AMPK activation inhibits NADPH oxidase (NOX) 2 expression, reactive oxygen species (ROS) generation, and pro-inflammatory cytokine secretion in macrophages during inflammation, while increased NOX2 expression is reported in experimental models of colitis and inflammatory bowel disease (IBD) patients. Although there are reductions in AMPK activity in IBD, it remains unclear whether targeted inhibition of NOX2 in the presence of defective AMPK can reduce the severity of colitis. Here, we investigate whether the inhibition of NOX2 ameliorates colitis in mice independent of AMPK activation. Our study identified that VAS2870 (a pan-Nox inhibitor) alleviated dextran sodium sulfate (DSS)-induced colitis in macrophage-specific AMPKβ1-deficient (AMPKβ1LysM) mice. Additionally, VAS2870 blocked LPS-induced TLR-4 and NOX2 expression, ROS production, nuclear translocation of NF-κB, and pro-inflammatory cytokine secretion in bone marrow-derived macrophages (BMDMs) from AMPKβ1LysM mice, whereas sodium salicylate (SS; AMPK β1 activator) did not. Both VAS2870 and SS inhibited LPS-induced NOX2 expression, ROS production, and pro-inflammatory cytokine secretions in bone marrow-derived macrophages (BMDMs) from wildtype (AMPKβ1fl/fl) mice but only VAS2870 inhibited these effects of LPSs in AMPKβ1LysM BMDMs. Furthermore, in macrophage cells (RAW 264.7), both SS and VAS2870 inhibited ROS production and the secretion of pro-inflammatory cytokines and reversed the impaired autophagy induced by LPSs. These data suggest that inhibiting NOX2 can reduce inflammation independent of AMPK in colitis.
Research Progress on New Environmentally Friendly Antifouling Coatings in Marine Settings: A Review
Authors: Liu De , Shu Haobo , Zhou Jiangwei , Bai Xiuqin , Cao Pan
Published: May 2023

Any equipment submerged in the ocean will have its surface attacked by fouling organisms, which can cause serious damage. Traditional antifouling coatings contain heavy metal ions, which also have a detrimental effect on the marine ecological environment and cannot fulfill the needs of practical applications. As the awareness of environmental protection is increasing, new environmentally friendly and broad-spectrum antifouling coatings have become the current research hotspot in the field of marine antifouling. This review briefly outlines the formation process of biofouling and the fouling mechanism. Then, it describes the research progress of new environmentally friendly antifouling coatings in recent years, including fouling release antifouling coatings, photocatalytic antifouling coatings and natural antifouling agents derived from biomimetic strategies, micro/nanostructured antifouling materials and hydrogel antifouling coatings. Highlights include the mechanism of action of antimicrobial peptides and the means of preparation of modified surfaces. This category of antifouling materials has broad-spectrum antimicrobial activity and environmental friendliness and is expected to be a new type of marine antifouling coating with desirable antifouling functions. Finally, the future research directions of antifouling coatings are prospected, which are intended to provide a reference for the development of efficient, broad-spectrum and green marine antifouling coatings.
Sleep and Lifestyle Habits of Medical and Non-Medical Students during the COVID-19 Lockdown
Authors: Pavlinac Dodig Ivana , Lusic Kalcina Linda , Demirovic Sijana , Pecotic Renata , Valic Maja , Dogas Zoran
Published: May 2023

It has been shown that the measures of social distancing and lockdown might have had negative effects on the physical and mental health of the population. We aim to investigate the sleep and lifestyle habits as well as the mood of Croatian medical (MS) and non-medical students (NMS) during the COVID-19 lockdown. The cross-sectional study included 1163 students (21.6% male), whose lifestyle and sleep habits and mood before and during the lockdown were assessed with an online questionnaire. The shift towards later bedtimes was more pronounced among NMS (~65 min) compared to MS (~38 min), while the shift toward later wake-up times was similar in both MS (~111 min) and NMS (~112 min). All students reported more frequent difficulty in falling asleep, night-time awakenings and insomnia (p < 0.001) during lockdown. A higher proportion of MS reported being less tired and less anxious during lockdown compared to pre-lockdown (p < 0.001). Both student groups experienced unpleasant moods and were less content during lockdown compared to the pre-lockdown period (p < 0.001). Our results emphasize the need for the promotion of healthy habits in the youth population. However, the co-appearance of prolonged and delayed sleep times along with decreased tiredness and anxiety among MS during lockdown reveals their significant workload during pre-lockdown and that even subtle changes in their day schedule might contribute to the well-being of MS.
Parents’ Perceptions of the Effectiveness of Music Therapy on Their Chronically Ill Children
Authors: Kobus Susann , Buehne Alexandra M. , Kathemann Simone , Buescher Anja K. , Lainka Elke
Published: May 2023

Chronic disease in a child, with the associated hospital stays, places considerable demands on the child and their family. The aim of this study was to investigate the parents’ perceptions of the music therapy used with their child during a hospital stay and to determine whether they felt that it reduced the child’s anxiety and stress generated by hospital admission. We hypothesized that the use of live music therapy from a music therapist would positively support these patients in everyday clinical practice, promote their wellbeing, and have positive impacts on their vital signs and blood pressure. Children with chronic gastroenterological and nephrological diseases included in this prospective study received live music therapy with a median duration of 41 min (range from 12 to 70 min) two to four times per week until discharged from the hospital. At the time of discharge, the parents were asked to complete a Likert-style questionnaire to evaluate the music therapy. Seven items were related to general questions about the patients and sessions, and eleven items evaluated the personal perceptions of the parents. Music therapy was conducted in 83 children, with a median age of 3 years (range from 1 month to 18 years). All parents (100%) completed the questionnaire at the time of discharge. Seventy-nine percent of the parents stated that their children were able to enjoy the music therapy sessions without being stressed. In addition, 98% of the respondents said that they were grateful for the music therapy their children received (97% fully agreed and 1% rather agreed). All parents considered music therapy to be beneficial for their child. The parents’ responses reflected the view that music therapy is beneficial to patients. According to the parents, music therapy can be integrated effectively in the inpatient clinical setting and can support children with chronic illnesses during their hospital stay.
Behaviors in Educational Settings during and after the COVID-19 Pandemic
Authors: Dong Xin , Ding Yi
Published: May 2023

This editorial is an introduction to the Special Issue “Behaviors in Educational Setting” [...]
The Effect of Social Networking Site Use on Depression in Graduate Students: The Mediating Role of Negative Social Comparison and Moderating Role of Implicit Personality Theories
Authors: Chen Zhenzhen , Wu Yang , Ma Hongyu , Niu Gengfeng , Wang Weixin
Published: May 2023

Objective: The current study aimed to investigate the effect of SNS use on graduate students’ depression and further explored the effect of negative social comparison and an individual’s implicit personality theory. Methods: Scales for Social Networking Site Use Intensity, the Negative Social Comparison Measure, the Implicit Personality Theory Inventory, and CES-D were used to investigate 1792 graduate students from a full-time university in Wuhan. Result: (1) Social networking site use was positively correlated with negative social comparison and depression; (2) the mediating effect of negative social comparison was significant in social networking site use’s influence on depression; (3) after controlling for negative social comparison, graduate students’ use of SNS could negatively predict depression; and (4) the mediation effect of negative social comparison was moderated by an individual’s implicit personality theory. Specifically, the mediation effect was more pronounced among the entity theorists, while the graduate students’ incremental implicit personality theory may buffer the depressive effect of negative social comparison. Conclusions: Negative social comparison mediates the relationship between SNS use and depression; in addition, individual differences in implicit personality theory (the entity theorist vs. incremental theorist) moderate the link between negative social comparison and depression.
Rehabilitation of Porous Building Components and Masonry by MICP Injection Method
Authors: Nagy Brigitte , Kustermann Andrea
Published: May 2023

Microbial-induced calcium carbonate precipitation (MICP) is a novel approach that is already being applied in various areas of construction. The precipitated calcium carbonate can be used to reduce porosity and thus increase the durability of deteriorated building components. This study investigates whether MICP injections are suitable for building rehabilitation. Porous mortar test samples of recycled aggregate and parts of deteriorated masonry were prepared. The MICP injections were performed without pressure and with an injection pump. The treatment effect was investigated after MICP injection by testing the porosity, strength and microscopic evaluation. It can be observed that multiple MICP injections under pressure result in a reduction of the pore volume of porous mortar samples. The produced calcium carbonate precipitates in the pore space of the samples and increases the density by 1.59% and the weight by 7.56%, which also results in a 48.3% reduction of the capillary water absorption. The results of strength tests show an increase of 45.16% in flexural strength and 35.64% in compressive strength compared with the untreated mortar samples. In addition, the MICP process was investigated and the precipitation was characterised. The X-ray diffraction (XRD) of the precipitated calcium carbonate confirms that mainly calcite was formed, which was also found in the pore structure of the MICP-injected masonry after the microscopic analysis. Precipitated calcium carbonate could be detected especially near the injection spots.
A BIM-Based Tool for Assessing Sustainability in Buildings Using the Green Pyramid Rating System
Authors: Marzouk Mohamed , Thabet Reham
Published: May 2023

Green construction management is an approach that aims to promote sustainable solutions in building design and construction. However, the complexity and variability in design scenarios often pose challenges to implementing these practices effectively. This study proposes a collaborative framework for green construction management that leverages automated and semi-automated simulations to facilitate performance-based decision making. The framework utilizes the green pyramid rating system (GPRS) as a third-party certification assessment provider. It employs various BIM analysis software tools, including Dynamo codes in Autodesk Revit and add-in plug-ins using Revit API, to extract information and parameters from the BIM model. The data are used to perform quantitative calculations and comprehensive analysis that aid decision makers in investigating, analyzing, improving, and assessing sustainability aspects throughout the project. The proposed framework enables a structured approach to green construction management that incorporates the building variable consecutive level of details/development (LOD) and life cycle phases. A case study of an office building in the new administrative capital in Egypt demonstrates the effectiveness of the framework in promoting sustainable solutions. The collaborative framework for interoperability design decisions proposed in this study provides a valuable tool for implementing green construction management practices. The use of automated and semi-automated simulations, following the GPRS, helps to ensure that sustainable solutions are advocated, interrogated, and refined throughout the project. The framework’s structured approach enables decision makers to investigate, analyze, improve, and assess sustainability aspects effectively.
On the Influence of Unexpected Earthquake Severity and Dampers Placement on Isolated Structures Subjected to Pounding Using the Modified Endurance Time Method
Authors: Majdi Ali , Sadeghi-Movahhed Ataallah , Mashayekhi Mohammadreza , Zardari Saeid , Benjeddou Omrane , De Domenico Dario
Published: May 2023

The aim of this study is to investigate the performance of isolated structures by considering the possibility of impact under severe earthquakes. In the design of isolated structures, the required displacement capacity is determined based on the considered earthquake hazard level. However, there is a possibility of an impact caused by moat walls or adjacent structures under severe earthquakes. Dampers are used in this study to improve the performance of structural and nonstructural components. In this regard, three isolated structures (6, 9, and 12 stories) equipped with Triple Friction Pendulum Isolator (TFPI) are designed under earthquake hazard levels of BSE-1 with return periods of 475 years. Based on the different positions of these three structures relative to each other, four scenarios are defined to investigate the effect of impact. Modified endurance time (MET) method, as a cost-efficient nonlinear time history analysis method, is employed for structural evaluation under variable earthquake hazard levels. The placement of dampers is also taken into account in evaluating the effect of dampers. Therefore, the structures have been retrofitted once by adding damping and stiffness devices (ADAS) on the stories and once by adding fluid viscous dampers (FVD) at the isolated level. Results indicate that structures might collapse under earthquake hazard levels of BSE-2 with return periods of 2475 years. This matter is influenced by the adjacency of two isolated structures next to each other, and the severity of this fact depends on the height of the structures and the displacement capacity of the isolators so that the tall, isolated structures have decreased the performance of the adjacent shorter isolated structure. Moreover, the placement of dampers has a significant influence on the performance of structural and nonstructural components, depending on the reason for the impact.
Facile Construction of Three-Dimensional Heterostructured CuCo2S4 Bifunctional Catalyst for Alkaline Water Electrolysis
Authors: Li Shengnan , Ma Pengli , Yang Jishuang , Krishnan Srinivasan , Kesavan Kannan S. , Xing Ruimin , Liu Shanhu
Published: May 2023

Developing an efficient multi-functional electrocatalyst with high efficiency and low cost to replace noble metals is significantly crucial for the industrial water electrolysis process and for producing sustainable green hydrogen (H2) fuel. Herein, ultrathin CuCo2S4 nanosheets assembled into highly open three-dimensional (3D) nanospheres of CuCo2S4 (Cu/Co = 33:67) were prepared by a facile one-pot solvothermal approach and utilized as a bifunctional electrocatalyst for efficient overall water splitting. The as-prepared CuCo2S4 is characterized structurally and morphologically; the BET surface area of the CuCo2S4 (Cu/Co = 33:67) catalyst was found to have a larger specific surface area (21.783 m2g−1) than that of other catalysts with a Cu/Co ratio of 67:33, 50:50, and 20:80. Benefiting from a highly open structure and ultrathin nanosheets with excellent exposure to catalytically active sites, CuCo2S4 (Cu/Co = 33:67) is identified as an efficient catalyst for the proton reduction and oxygen evolution reactions in 1 M KOH with an overpotential of 182 and 274 mV at 10 mA cm−2, respectively. As expected, a low cell voltage of 1.68 V delivers a current density of 10 mA cm−2. Stability and durability are also greatly enhanced under harsh alkaline conditions. Therefore, this work provides a simple strategy for the rational design of spinel-based transition metal sulfide catalysts for electrocatalysis.
Reaction Mechanism Development for Methane Steam Reforming on a Ni/Al2O3 Catalyst
Authors: Richter Jana , Rachow Fabian , Israel Johannes , Roth Norbert , Charlafti Evgenia , Günther Vivien , Flege Jan Ingo , Mauss Fabian
Published: May 2023

In this work, a reliable kinetic reaction mechanism was revised to accurately reproduce the detailed reaction paths of steam reforming of methane over a Ni/Al2O3 catalyst. A steady-state fixed-bed reactor experiment and a 1D reactor catalyst model were utilized for this task. The distinctive feature of this experiment is the possibility to measure the axially resolved temperature profile of the catalyst bed, which makes the reaction kinetics inside the reactor visible. This allows for understanding the actual influence of the reaction kinetics on the system; while pure gas concentration measurements at the catalytic reactor outlet show near-equilibrium conditions, the inhere presented temperature profile shows that it is insufficient to base a reaction mechanism development on close equilibrium data. The new experimental data allow for achieving much higher quality in the modeling efforts. Additionally, by carefully controlling the available active surface via dilution in the experiment, it was possible to slow down the catalyst conversion rate, which helped during the adjustment of the reaction kinetics. To assess the accuracy of the revised mechanism, a monolith experiment from the literature was simulated. The results show that the fitted reaction mechanism was able to accurately predict the experimental outcomes for various inlet mass flows, temperatures, and steam-to-carbon ratios.
Highly Active Carbon Material Derived from Carica papaya Fruit Juice: Access to Efficient Photocatalytic Degradation of Methylene Blue in Aqueous Solution under the Illumination of Ultraviolet Light
Authors: Bhatti Muhammad Ali , Dawi Elmuez , Tahira Aneela , Almani Khalida Faryal , Medany Shymaa S. , Nafady Ayman , Solangi Zulifqar Ali , Aftab Umair , Ibhupoto Zaffar Hussain
Published: May 2023

Herein, we describe a cost-effective, efficient, sustainable, and environmentally friendly pyrolytic method for the synthesis of highly active carbon materials from Carica papaya fruit juice for the photodegradation of various pollutants, such as methylene blue (MB), in aqueous solutions using ultraviolet (UV) light. Various analytical techniques were used to examine the morphology, crystal quality, functional group chemistry, particle size distribution, and optical properties of the materials. For evaluating the performance of the newly prepared carbon material, various photocatalyst parameters were investigated, including initial dye concentration, catalyst dose, pH of dye solution, cyclic stability, and scavenger studies. The obtained findings attest that the optimal degradation efficiency of carbon material for high MB concentrations (2.3 × 10−5 M) is around 98.08%, whereas at low concentrations of MB (1.5 × 10−5 M) it reaches 99.67%. Degradation kinetics indicate that MB degrades in a first-order manner. Importantly, as the pH of the dye solution was adjusted to ~11, the degradation rate increased significantly. The scavenger study indicated that hydroxyl radicals were the predominant species involved in the degradation of MB. In addition, active surface site exposure and charge transfer were strongly associated with efficient MB degradation. On the basis of its performance, this newly developed carbon material may prove to be an excellent alternative and promising photocatalyst for wastewater treatment. Furthermore, the synthetic approach used to produce carbon material from Carica papaya fruit juice may prove useful for the development of a new generation of photoactive materials for environmentally friendly applications, as well as for the production of hydrogen from solar energy.
The Studies of Sepharose-Immobilized Lipases: Combining Techniques for the Enhancement of Activity and Thermal Stability
Authors: Siódmiak Tomasz , Dulęba Jacek , Haraldsson Gudmundur G. , Siódmiak Joanna , Marszałł Michał Piotr
Published: May 2023

Thermal stability is one of the essential parameters characterizing biocatalysts with potential applications in the chemical and pharmaceutical industries. Therefore, it is extremely important to develop standardized procedures for enzyme stability studies. The paper attempts to assess the thermal stability of immobilized lipases in aqueous buffers: lipase B from Candida antarctica (CALB) and lipase from Candida rugosa (CRL-OF) immobilized on the Octyl-Sepharose CL-4B carrier. As part of the optimization conditions of the immobilization, the influence of time on the catalytic activity and lipase loading, as well as the effect of temperature on lipase activity (optimal incubation—14 h at 4 °C), was determined. The thermal stability test procedure was carried out for 7 days using a climatic chamber (65 °C) and a refrigerator (4 °C). The studies of immobilized lipases included the assessment of the impact of various solvents (water, citrate buffer, 1,2-dichloropropane—DCP), temperature, light in the visible spectral range (400–800 nm), and additions of calcium ions. The highest value of residual activity (564.5 ± 21.6%) was received by storing the immobilized CALB in citrate buffer (pH 4.0, 500 mM) with the addition of calcium ions (Ca2+). On the other hand, residual activity values for immobilized CRL-OF after storage in the climatic chamber were lower than 5%. A combining of techniques: immobilization onto the support in high ionic strength and low pH, with a technique of extremally high-temperature applied in a climatic chamber, with the addition of Ca2+ allowed to achieve of excellent thermal stability of the immobilized CALB, with increasing of catalytic activity more than five-fold. Additionally, performing studies on the thermal stability of the tested lipases using a climatic chamber seems to be particularly promising in the context of unifying and standardizing storage guidelines, enabling the comparison of results between different laboratories, as well as enhancing catalytic activity.
Conversion of Unmodified Stem Cells to Pacemaker Cells by Overexpression of Key Developmental Genes
Authors: Karimi Tahereh , Pan Zhizhong , Potaman Vladimir N. , Alt Eckhard U.
Published: May 2023

Arrhythmias of the heart are currently treated by implanting electronic pacemakers and defibrillators. Unmodified adipose tissue-derived stem cells (ASCs) have the potential to differentiate into all three germ layers but have not yet been tested for the generation of pacemaker and Purkinje cells. We investigated if—based on overexpression of dominant conduction cell-specific genes in ASCs—biological pacemaker cells could be induced. Here we show that by overexpression of certain genes that are active during the natural development of the conduction system, the differentiation of ASCs to pacemaker and Purkinje-like cells is feasible. Our study revealed that the most effective procedure consisted of short-term upregulation of gene combinations SHOX2-TBX5-HCN2, and to a lesser extent SHOX2-TBX3-HCN2. Single-gene expression protocols were ineffective. Future clinical implantation of such pacemaker and Purkinje cells, derived from unmodified ASCs of the same patient, could open up new horizons for the treatment of arrythmias.
Flexible Humidity Sensor Based on Au Nanoparticles/Organosilica-Containing Polyelectrolyte Composite
Authors: Su Pi-Guey , Hsu Chih-Chang
Published: May 2023

A novel flexible humidity sensor incorporating gold nanoparticles (Au NPs) and a trifunctional organosilica compound has been developed through the integration of sol–gel processing, free radical polymerization, and self-assembly techniques. The trifunctional organosilica was initially synthesized by modifying (3-mercaptopropyl)trimethoxysilane (thiol-MPTMS) with 3-(trimethoxysilyl)propyl methacrylate (vinyl-TMSPMA). Subsequently, a hydrophilic polyelectrolyte, [3(methacryloylamino)propyl]trimethyl ammonium chloride (MAPTAC), was grafted onto the MPTMS-TMSPMA gel. The Au NPs were assembled onto the thiol groups present in the MPTMS-TMSPMA-MAPTAC gel network. The compositional and microstructural properties of the Au NPs/MPTMS-TMSPMA-MAPTAC composite film were investigated utilizing Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). The presence of thiol groups and mesoporous silica skeletons ensured the stability of the humidity-sensing film on the substrate under highly humid conditions, while the hydrophilic groups functioned as humidity-sensitive sites. This innovative humidity sensor demonstrated high sensitivity, acceptable linearity, minimal hysteresis, and rapid response time across a broad range of working humidity levels. Based on the complex impedance spectra analysis, hydronium ions (H3O+) were determined to govern the conductance process of the flexible humidity sensor.
CNS Border-Associated Macrophages: Ontogeny and Potential Implication in Disease
Authors: Dermitzakis Iasonas , Theotokis Paschalis , Evangelidis Paschalis , Delilampou Efthymia , Evangelidis Nikolaos , Chatzisavvidou Anastasia , Avramidou Eleni , Manthou Maria Eleni
Published: May 2023

Being immune privileged, the central nervous system (CNS) is constituted by unique parenchymal and non-parenchymal tissue-resident macrophages, namely, microglia and border-associated macrophages (BAMs), respectively. BAMs are found in the choroid plexus, meningeal and perivascular spaces, playing critical roles in maintaining CNS homeostasis while being phenotypically and functionally distinct from microglial cells. Although the ontogeny of microglia has been largely determined, BAMs need comparable scrutiny as they have been recently discovered and have not been thoroughly explored. Newly developed techniques have transformed our understanding of BAMs, revealing their cellular heterogeneity and diversity. Recent data showed that BAMs also originate from yolk sac progenitors instead of bone marrow-derived monocytes, highlighting the absolute need to further investigate their repopulation pattern in adult CNS. Shedding light on the molecular cues and drivers orchestrating BAM generation is essential for delineating their cellular identity. BAMs are receiving more attention since they are gradually incorporated into neurodegenerative and neuroinflammatory disease evaluations. The present review provides insights towards the current understanding regarding the ontogeny of BAMs and their involvement in CNS diseases, paving their way into targeted therapeutic strategies and precision medicine.
Interplay between Fe(II) and Fe(III) and Its Impact on Thermoelectric Properties of Iron-Substituted Colusites Cu26−xFexV2Sn6S32
Authors: Polevik Alexey O. , Sobolev Alexey V. , Glazkova Iana S. , Presniakov Igor A. , Verchenko Valeriy Yu. , Link Joosep , Stern Raivo , Shevelkov Andrei V.
Published: May 2023

Following the trend of finding better thermoelectric materials among synthetic analogs of copper–chalcogenide minerals, we have synthesized iron-bearing colusites of a general formula Cu26−xFexV2Sn6S32. They crystallize in the cubic space group P-43n with the unit cell parameter increasing linearly with the iron content. At a low iron concentration, the crystal structure features disorder manifested by an anti-site effect and a shift of a part of the tin atoms from their ideal positions, which is absent for higher iron contents. The magnetization and 57Fe/119Sn Mössbauer studies showed that, for x = 1, iron is present as Fe3+, whereas for x > 1, Fe2+ and Fe3+ coexist. Additionally, weak antiferromagnetic interactions between iron atoms and fast on the 57Fe Mössbauer time scale (107–109 s−1) electron transfer between adjacent Fe2+ and Fe3+ centers were revealed. Thermoelectric studies showed that iron-bearing colusites are p-type semiconductors with low thermal conductivity stemming from their complex crystal structure and structural disorder. The highest ZT of 0.78 at 700 K was found for the x = 1 iron content, where iron is present as Fe3+ only.
DNA Barcoding of Black Flies (Diptera: Simuliidae) in Slovakia and Its Utility for Species Identification
Authors: Kúdelová Tatiana , Krčmárik Samuel , Lužáková Ivona , Bujačková Bibiana , Matická Karin , Kúdela Matúš
Published: May 2023

DNA barcoding based on the cytochrome oxidase I gene is increasingly used in black flies (Diptera: Simuliidae), but extensive data for larger areas are still rare. Slovakia, with well-explored black fly fauna, was chosen to verify the reliability of DNA barcoding for species identification. The DNA barcoding region of the COI gene of 235 individuals of 25 black fly species from Slovakia was sequenced. Among them, 30 sequence clusters with assigned Barcode Index Numbers (BINs) were identified, and 5 of them were recorded for the first time. The average intraspecific genetic divergence was 0–3.24%, whereas the average interspecific divergence was 12.3–17.8%. Based on the barcode sequence, 14 species could be identified unambiguously, and 3 of them (Prosimulium latimucro, Simulium costatum, S. degrangei) are split into two or more barcode clusters. In eleven species, some degree of barcode sharing occurred, often combined with barcode splitting. The results showed hidden diversity as well as cases of shared barcode sequences among the studied species. Further investigation using other molecular markers is necessary due to the overlap of intraspecific and interspecific variability.
An Efficient Deep Learning for Thai Sentiment Analysis
Authors: Khamphakdee Nattawat , Seresangtakul Pusadee
Published: May 2023

The number of reviews from customers on travel websites and platforms is quickly increasing. They provide people with the ability to write reviews about their experience with respect to service quality, location, room, and cleanliness, thereby helping others before booking hotels. Many people fail to consider hotel bookings because the numerous reviews take a long time to read, and many are in a non-native language. Thus, hotel businesses need an efficient process to analyze and categorize the polarity of reviews as positive, negative, or neutral. In particular, low-resource languages such as Thai have greater limitations in terms of resources to classify sentiment polarity. In this paper, a sentiment analysis method is proposed for Thai sentiment classification in the hotel domain. Firstly, the Word2Vec technique (the continuous bag-of-words (CBOW) and skip-gram approaches) was applied to create word embeddings of different vector dimensions. Secondly, each word embedding model was combined with deep learning (DL) models to observe the impact of each word vector dimension result. We compared the performance of nine DL models (CNN, LSTM, Bi-LSTM, GRU, Bi-GRU, CNN-LSTM, CNN-BiLSTM, CNN-GRU, and CNN-BiGRU) with different numbers of layers to evaluate their performance in polarity classification. The dataset was classified using the FastText and BERT pre-trained models to carry out the sentiment polarity classification. Finally, our experimental results show that the WangchanBERTa model slightly improved the accuracy, producing a value of 0.9225, and the skip-gram and CNN model combination outperformed other DL models, reaching an accuracy of 0.9170. From the experiments, we found that the word vector dimensions, hyperparameter values, and the number of layers of the DL models affected the performance of sentiment classification. Our research provides guidance for setting suitable hyperparameter values to improve the accuracy of sentiment classification for the Thai language in the hotel domain.
Colon Disease Diagnosis with Convolutional Neural Network and Grasshopper Optimization Algorithm
Authors: Mohamed Amna Ali A. , Hançerlioğullari Aybaba , Rahebi Javad , Ray Mayukh K. , Roy Sudipta
Published: May 2023

This paper presents a robust colon cancer diagnosis method based on the feature selection method. The proposed method for colon disease diagnosis can be divided into three steps. In the first step, the images’ features were extracted based on the convolutional neural network. Squeezenet, Resnet-50, AlexNet, and GoogleNet were used for the convolutional neural network. The extracted features are huge, and the number of features cannot be appropriate for training the system. For this reason, the metaheuristic method is used in the second step to reduce the number of features. This research uses the grasshopper optimization algorithm to select the best features from the feature data. Finally, using machine learning methods, colon disease diagnosis was found to be accurate and successful. Two classification methods are applied for the evaluation of the proposed method. These methods include the decision tree and the support vector machine. The sensitivity, specificity, accuracy, and F1Score have been used to evaluate the proposed method. For Squeezenet based on the support vector machine, we obtained results of 99.34%, 99.41%, 99.12%, 98.91% and 98.94% for sensitivity, specificity, accuracy, precision, and F1Score, respectively. In the end, we compared the suggested recognition method’s performance to the performances of other methods, including 9-layer CNN, random forest, 7-layer CNN, and DropBlock. We demonstrated that our solution outperformed the others.
Plasma Proteomic Analysis Reveals the Potential Role of Lectin and Alternative Complement Pathways in IgA Vasculitis Pathogenesis
Authors: Demir Selcan , Yet Idil , Sardan Ekiz Melis , Sag Erdal , Bilginer Yelda , Celikbicak Omur , Lay Incilay , Ozen Seza
Published: May 2023

Background: IgA vasculitis (IgAV) is the most common form of childhood vasculitis. A better understanding of its pathophysiology is required to identify new potential biomarkers and treatment targets. Objective: to assess the underlying molecular mechanisms in the pathogenesis of IgAV using an untargeted proteomics approach. Methods: Thirty-seven IgAV patients and five healthy controls were enrolled. Plasma samples were collected on the day of diagnosis before any treatment was initiated. We used nano-liquid chromatography–tandem mass spectrometry (nLC–MS/MS) to investigate the alterations in plasma proteomic profiles. For the bioinformatics analyses, databases including Uniprot, PANTHER, KEGG, Reactome, Cytoscape, and IntAct were used. Results: Among the 418 proteins identified in the nLC–MS/MS analysis, 20 had significantly different expressions in IgAV patients. Among them, 15 were upregulated and 5 were downregulated. According to the KEGG pathway and function classification analysis, complement and coagulation cascades were the most enriched pathways. GO analyses showed that the differentially expressed proteins were mainly involved in defense/immunity proteins and the metabolite interconversion enzyme family. We also investigated molecular interactions in the identified 20 proteins of IgAV patients. We extracted 493 interactions from the IntAct database for the 20 proteins and used Cytoscape for the network analyses. Conclusion: Our results clearly suggest the role of the lectin and alternate complement pathways in IgAV. The proteins defined in the pathways of cell adhesion may serve as biomarkers. Further functional studies may lead the way to better understanding of the disease and new therapeutic options for IgAV treatment.