bluuebunny/crossref_metadata_2025 huggingface dataset:
CBP/p300 Bromodomain Inhibitor–I–CBP112 Declines Transcription of the Key ABC Transporters and Sensitizes Cancer Cells to Chemotherapy Drugs
Authors: Strachowska Magdalena , Gronkowska Karolina , Michlewska Sylwia , Robaszkiewicz Agnieszka
Published: September 2021

The high expression of some ATP-binding cassette (ABC) transporters is linked to multidrug resistance in cancer cells. We aimed to determine if I-CBP112, which is a CBP/p300 bromodomain inhibitor, altered the vulnerability of the MDA-MB-231 cell line to chemotherapy drugs, which are used in neoadjuvant therapy in patients with triple negative breast cancer (TNBC). MDA-MB-231 cells represent TNBC, which is negative for the expression of estrogen and progesterone receptors and HER2 protein. An I-CBP112-induced decrease in the expression of all the studied ABCs in the breast, but also in the lung (A549), and hepatic (HepG2) cancer cell lines was associated with increased accumulation of doxorubicin, daunorubicin, and methotrexate inside the cells as well as with considerable cell sensitization to a wide range of chemotherapeutics. Gene promoters repressed by I-CBP112 in MDA-MB-231 cells, such as ABCC1 and ABCC10, were characterized by enhanced nucleosome acetylation and, simultaneously, by considerably lower trimethylation in the transcription-promoting form of H3K4me3. The CBP/p300 bromodomain inhibitor induced the recruitment of LSD1 to the gene promoters. The inhibition of this demethylase in the presence of I-CBP112 prevented the repression of ABCC1 and ABCC10 and, to a considerable extent, cancer cells’ sensitization to drugs. In conclusion, the CBP/p300 bromodomain inhibitor I-CBP112 can be considered as a potent anti-multidrug-resistance agent, capable of repressing key ABC transporters responsible for drug efflux in various cancer types.
The Emerging Functions of Circular RNAs in Bladder Cancer
Authors: Sun Kai , Wang Di , Yang Burton B. , Ma Jian
Published: September 2021

Bladder cancer (BC) is among the top ten most common cancer types worldwide and is a serious threat to human health. Circular RNAs (circRNAs) are a new class of non-coding RNAs generated by covalently closed loops through back-splicing. As an emerging research hotspot, circRNAs have attracted considerable attention due to their high conservation, stability, abundance, and specificity of tissue development. Accumulating evidence has revealed different form of circRNAs are closely related to the malignant phenotype, prognosis and chemotherapy resistance of BC, suggesting that different circRNAs may be promising biomarkers and have therapeutic significance in BC. The intention of this review is to summarize the mechanisms of circRNA-mediated BC progression and their diagnostic and prognostic value as biomarkers, as well as to further explore their roles in chemotherapy resistance.
Technical Challenges for CTC Implementation in Breast Cancer
Authors: Ramos-Medina Rocío , López-Tarruella Sara , del Monte-Millán María , Massarrah Tatiana , Martín Miguel
Published: September 2021

Breast cancer is the most common neoplasm in women worldwide. Tissue biopsy, currently the gold standard to obtain tumor molecular information, is invasive and might be affected by tumor heterogeneity rendering it incapable to portray the complete dynamic picture by the absence of specific genetic changes during the evolution of the disease. In contrast, liquid biopsy can provide unique opportunities for real-time monitoring of disease progression, treatment response and for studying tumor heterogeneity combining the information of DNA that tumors spread in the blood (circulating tumor DNA) with CTCs analysis. In this review, we analyze the technical and biological challenges for isolation and characterization of circulating tumor cells from breast cancer patients. Circulating tumor cell (CTC) enumeration value is included in numerous clinical studies due to the prognostic’s role of these cells. Despite this, there are so many questions pending to answer. How to manage lymphocytes background, how to distinguish the CTCs subtypes or how to work with frozen samples, are some of the issues that will discuss in this review. Based on our experience, we try to address these issues and other technical limitations that should be solved to optimize the standardization of protocols, sample extraction procedures, circulating-tumor material isolation (CTCs vs. ctDNA) and the very diverse methodologies employed, aiming to consolidate the use of CTCs in the clinic. Furthermore, we think that new approaches focusing on isolation CTCs in other body fluids such as cerebrospinal or ascitic fluid are necessary to increase the opportunities of circulating tumor cells in the practice clinic as well as to study the promising role of CTC clusters and their prognostic value in metastatic breast cancer.
Responses to SARS-CoV-2 Vaccination in Patients with Cancer (ReCOVer Study): A Prospective Cohort Study of the Hellenic Cooperative Oncology Group
Authors: Linardou Helena , Spanakis Nikolaos , Koliou Georgia-Angeliki , Christopoulou Athina , Karageorgopoulou Sofia , Alevra Nephely , Vagionas Anastasios , Tsoukalas Nikolaos , Sgourou Stavroula , Fountzilas Elena , Sgouros Joseph , Razis Evangelia , Chatzokou Dimitra , Lampaki Sofia , Res Eleni , Saridaki Zacharenia , Mountzios Giannis , Saroglou George , Fountzilas George
Published: September 2021

Data on the effectiveness and safety of approved SARS-CoV-2 vaccines in cancer patients are limited. This observational, prospective cohort study investigated the humoral immune response to SARS-CoV-2 vaccination in 232 cancer patients from 12 HeCOG-affiliated oncology departments compared to 100 healthcare volunteers without known active cancer. The seropositivity rate was measured 2–4 weeks after two vaccine doses, by evaluating neutralising antibodies against the SARS-CoV-2 spike protein using a commercially available immunoassay. Seropositivity was defined as ≥33.8 Binding-Antibody-Units (BAU)/mL. A total of 189 patients and 99 controls were eligible for this analysis. Among patients, 171 (90.5%) were seropositive after two vaccine doses, compared to 98% of controls (p = 0.015). Most seronegative patients were males (66.7%), >70-years-old (55.5%), with comorbidities (61.1%), and on active treatment (88.9%). The median antibody titers among patients were significantly lower than those of the controls (523 vs. 2050 BAU/mL; p < 0.001). The rate of protective titers was 54.5% in patients vs. 97% in controls (p < 0.001). Seropositivity rates and IgG titers in controls did not differ for any studied factor. In cancer patients, higher antibody titers were observed in never-smokers (p = 0.006), women (p = 0.022), <50-year-olds (p = 0.004), PS 0 (p = 0.029), and in breast or ovarian vs. other cancers. Adverse events were comparable to registration trials. In this cohort study, although the seropositivity rate after two vaccine doses in cancer patients seemed satisfactory, their antibody titers were significantly lower than in controls. Monitoring of responses and further elucidation of the clinical factors that affect immunity could guide adaptations of vaccine strategies for vulnerable subgroups.
A Perspective on the Role of Microbiome for Colorectal Cancer Treatment
Authors: Kalasabail Sanjna , Engelman Jared , Zhang Linda Yun , El-Omar Emad , Yim Howard Chi Ho
Published: September 2021

In healthy hosts, trillions of microbes colonise the gut and oral cavity in a well-balanced state, maintaining a mutually beneficial relationship. Loss of this balance, termed dysbiosis, is strongly implicated in the pathogenesis of colorectal cancer (CRC). However, the roles of microbiota and dysbiosis in CRC treatment remain poorly understood. Recent studies suggest that the gut microbiota has the ability to affect the host response to chemotherapeutic agents by enhancing drug efficacy, promoting chemoresistance and mediating chemotherapy-induced toxicity and side effects via a variety of mechanisms. Several other studies have also proposed manipulation of the microbiota to optimise CRC treatment. In this review, we summarise the current advancement of knowledge on how microbiota and CRC treatments interact with each other and how this interaction may shed some light on the development of personalised microbiota manipulations that improve CRC treatment outcomes.
Risk Factors for Metachronous Isolated Peritoneal Metastasis after Preoperative Chemotherapy and Potentially Curative Gastric Cancer Resection: Results from the CRITICS Trial
Authors: Caspers Irene A. , Sikorska Karolina , Slagter Astrid E. , van Amelsfoort Romy M. , Meershoek-Klein Kranenbarg Elma , van de Velde Cornelis J. H. , Lind Pehr , Nordsmark Marianne , Jansen Edwin P. M. , Verheij Marcel , van Sandick Johanna W. , Cats Annemieke , van Grieken Nicole C. T.
Published: September 2021

Gastric cancer (GC) patients at high risk of developing peritoneal metastasis (PM) as a single site of metastasis after curative treatment may be candidates for adjuvant prophylactic strategies. Here we investigated risk factors for metachronous isolated PM in patients who were treated in the CRITICS trial (NCT00407186). Univariable and multivariable analyses on both metachronous isolated PM and ‘other events’, i.e., (concurrent) distant metastasis, locoregional recurrence or death, were performed using a competing risk model and summarized by cumulative incidences. Isolated PM occurred in 64 of the 606 (11%) included patients. Diffuse or mixed histological subtype, ypT4 tumor stage and LNhigh (ypN3 lymph node stage or a lymph node ratio >20%) were independent risk factors for isolated PM in both univariable and multivariable analyses. Likewise, LNhigh was an independent risk factor for ‘other events’. Patients with tumors who were positive for all three independent risk factors had the highest two-year cumulative incidence of 43% for isolated PM development. In conclusion, diffuse or mixed histological subtype, ypT4 and LNhigh were identified as independent risk factors for isolated PM in patients treated with preoperative chemotherapy followed by surgical resection. The combination of these factors may identify a subgroup that may benefit from PM-preventing treatment strategies.
Diagnostic Strategies for Thyroid Nodules Based on Ultrasonographic Findings in Japan
Authors: Shimura Hiroki , Matsumoto Yoshiko , Murakami Tsukasa , Fukunari Nobuhiro , Kitaoka Masafumi , Suzuki Shinichi
Published: September 2021

In recent years, the incidence of thyroid cancer has been increasing worldwide, which is believed to be mainly due to the widespread use of imaging examinations, such as ultrasonography. In this context, ultrasonography has become increasingly important because it can evaluate not only the presence or absence of nodules, but also the detailed characteristics of the nodule, making it possible to diagnose benign or malignant nodules before cytology is performed. In Japan, the third edition of the sonographic diagnostic criteria for thyroid nodules is currently widely used, and its content is similar to that of recent meta-analyses and guidelines from medical societies in other countries. In addition, since overdiagnosis of very-low-risk thyroid cancer has recently become an issue, criteria for the implementation of fine needle aspiration cytology (FNAC) have been published by various countries. The Japan Society of Breast and Thyroid Sonology provides guidelines for FNAC implementation for solid and cystic nodules. In the United States, the ATA, NCCA, and ACR have published guidelines, whereas in Europe, the ESMO and ETA have done the same. All of these guidelines used to classify risk are based on nodule size and sonographic findings. This article outlines the diagnostic criteria and management guidelines in Japan in comparison with those published in other countries. Case studies using actual images were also performed to examine the differences in the FNAC guidelines.
Kinetic Study on Microwave-Assisted Oligomerization of 1-Decene over a HY Catalyst
Authors: Echaroj Snunkhaem , Asavatesanupap Channarong , Chavadej Sumaeth , Santikunaporn Malee
Published: September 2021

A promising production route for a high-quality base stock for lubricants is the oligomerization of high molecular-weight olefins in a high energy efficiency system. Oligomerization of 1-decene (C10) was conducted in a microwave-assisted system over a HY zeolite catalyst at different reaction temperatures and times. Higher reaction temperature resulted in increasing formation of dimers and trimers. The oligomerization reaction yielded 80% conversion, 54.2% dimer product, 22.3% trimer product and 3.4% heavier product at 483 K for a reaction time of 3 h. The best fit kinetic model for the dimerization reaction was formulated from an assumption of no vacant reaction sites. For the trimerization reaction, a molecule of dimer (C20) formed on the active site, interacted with a molecule of 1-decene in the bulk solution to form a molecule of trimer (C30). Apparent activation energies for the dimerization and trimerization reactions were 70.8 ± 0.8 and 83.6 ± 0.9 kJ/mol, respectively. The C13-NMR spectrum indicated that the oligomer product contained a significant portion of highly branched hydrocarbons, causing a substantial reduction in the viscosity index compared to conventional poly-alpha olefin lubricant (PAO).
The Saga of Endocrine FGFs
Authors: Phan Phuc , Saikia Bibhuti Ballav , Sonnaila Shivakumar , Agrawal Shilpi , Alraawi Zeina , Kumar Thallapuranam Krishnaswamy Suresh , Iyer Shilpa
Published: September 2021

Fibroblast growth factors (FGFs) are cell-signaling proteins with diverse functions in cell development, repair, and metabolism. The human FGF family consists of 22 structurally related members, which can be classified into three separate groups based on their action of mechanisms, namely: intracrine, paracrine/autocrine, and endocrine FGF subfamilies. FGF19, FGF21, and FGF23 belong to the hormone-like/endocrine FGF subfamily. These endocrine FGFs are mainly associated with the regulation of cell metabolic activities such as homeostasis of lipids, glucose, energy, bile acids, and minerals (phosphate/active vitamin D). Endocrine FGFs function through a unique protein family called klotho. Two members of this family, α-klotho, or β-klotho, act as main cofactors which can scaffold to tether FGF19/21/23 to their receptor(s) (FGFRs) to form an active complex. There are ongoing studies pertaining to the structure and mechanism of these individual ternary complexes. These studies aim to provide potential insights into the physiological and pathophysiological roles and therapeutic strategies for metabolic diseases. Herein, we provide a comprehensive review of the history, structure–function relationship(s), downstream signaling, physiological roles, and future perspectives on endocrine FGFs.
Anticryptosporidium Efficacy of Olea europaea and Ficus carica Leaves Extract in Immunocompromised Mice Associated with Biochemical Characters and Antioxidative System
Authors: Abd El-Hamed Wafaa Fayez , Yousef Nahed Samy , Mazrou Yasser S. A. , Elkholy Walaa A. E. S. , El-Refaiy Amal I. , Elfeky Faten A. , Albadrani Muayad , El-Tokhy Ahmed I. , Abdelaal Khaled
Published: September 2021

Cryptosporidiosis is caused by an opportunistic protozoan parasite (Cryptosporidium parvum and C. hominis) known as a parasite of humans, especially children and immunocompromised patients. The current study was designed to evaluate the therapeutic efficacy of a mixture of fig and olive leaf extracts as an alternative medicinal plant. Parasitological examination for oocysts in the stool and histopathological alterations in the small intestines were examined. Additionally, biochemical analyses of liver and kidney functions in addition to antioxidant parameters such as superoxide dismutase (SOD), glutathione peroxidase (GSH) and catalase (CAT) in the plasma were evaluated. Our results showed that marked reduction in oocysts shedding and amelioration in intestinal histopathological changes and hepatic or renal functions were detected in all treated groups compared to the control infected group. Additionally, the treated groups with tested extracts at ratios 1:3 and 1:5 showed a significant decrease in the number of oocysts compared to the other treated groups. Results exhibited a significant increase in the plasma SOD, CAT and GSH levels in treated groups compared to the infected control one. This study suggested that a mixture of fig and olive leaf extracts is a convenient promising therapeutic agent for Cryptosporidiosis.
Role of Complement in Regulating Inflammation Processes in Renal and Prostate Cancers
Authors: Netti Giuseppe Stefano , Franzin Rossana , Stasi Alessandra , Spadaccino Federica , Dello Strologo Andrea , Infante Barbara , Gesualdo Loreto , Castellano Giuseppe , Ranieri Elena , Stallone Giovanni
Published: September 2021

For decades, the complement system, the central pillar of innate immune response, was recognized as a protective mechanism against cancer cells and the manipulation of complement effector functions in cancer setting offered a great opportunity to improve monoclonal antibody-based cancer immunotherapies. Similarly, cellular senescence, the process of cell cycle arrest that allow DNA and tissue repair has been traditionally thought to be able to suppress tumor progression. However, in recent years, extensive research has identified the complement system and cellular senescence as two main inducers of tumour growth in the context of chronic, persistent inflammation named inflammaging. Here, we discuss the data describing the ambivalent role of senescence in cancer with a particular focus on tumors that are strongly dependent on complement activation and can be understood by a new, senescence-related point of view: prostate cancer and renal cell carcinoma.
Brain Immune Interactions—Novel Emerging Options to Treat Acute Ischemic Brain Injury
Authors: Muhammad Sajjad , Chaudhry Shafqat Rasul , Kahlert Ulf Dietrich , Niemelä Mika , Hänggi Daniel
Published: September 2021

Ischemic stroke is still among the leading causes of mortality and morbidity worldwide. Despite intensive advancements in medical sciences, the clinical options to treat ischemic stroke are limited to thrombectomy and thrombolysis using tissue plasminogen activator within a narrow time window after stroke. Current state of the art knowledge reveals the critical role of local and systemic inflammation after stroke that can be triggered by interactions taking place at the brain and immune system interface. Here, we discuss different cellular and molecular mechanisms through which brain–immune interactions can take place. Moreover, we discuss the evidence how the brain influence immune system through the release of brain derived antigens, damage-associated molecular patterns (DAMPs), cytokines, chemokines, upregulated adhesion molecules, through infiltration, activation and polarization of immune cells in the CNS. Furthermore, the emerging concept of stemness-induced cellular immunity in the context of neurodevelopment and brain disease, focusing on ischemic implications, is discussed. Finally, we discuss current evidence on brain–immune system interaction through the autonomic nervous system after ischemic stroke. All of these mechanisms represent potential pharmacological targets and promising future research directions for clinically relevant discoveries.
L-Plastin Phosphorylation: Possible Regulation by a TNFR1 Signaling Cascade in Osteoclasts
Authors: Chellaiah Meenakshi A.
Published: September 2021

Tumor necrosis factor-alpha (TNF-α) signaling regulates phosphorylation of L-plastin, which is involved in forming the nascent sealing zone, a precursor zone for the matured sealing ring. This study aimed to illustrate the molecular mechanisms of L-plastin phosphorylation and the subsequent formation of the nascent sealing zone in osteoclasts treated with TNF-α. Here, we report that anti-TNF-receptor 1, inhibitors of signaling proteins (Src, PI3-K, Rho, and Rho-kinase), and siRNA of TRAF-6 attenuated the phosphorylation of LPL and filamentous actin content significantly in the presence of TNF-α. An inhibitor of integrin αvβ3, PKC, or PKA did not inhibit TNF-α-induced L-plastin phosphorylation. Inhibitors of Src and PI3-K and not Rho or Rho-kinase reduced tyrosine phosphorylation of TRAF-6, suggesting that Src and PI3-K regulate TRAF-6 phosphorylation, and Rho and Rho-kinase are downstream of TRAF-6 regulation. Osteoclasts expressing constitutively active or kinase-defective Src proteins were used to determine the role of Src on L-plastin phosphorylation; similarly, the effect of Rho was confirmed by transducing TAT-fused constitutively active (V14) or dominant-negative (N19) Rho proteins into osteoclasts. Pull-down analysis with glutathione S-transferase-fused SH2 and SH3 domains of Src and PI3-K demonstrated coprecipitation of L-plastin and TRAF-6 with the SH3 and SH2 domains of the PI3-K and Src proteins. However, the actual order of the interaction of proteins requires further elucidation; a comprehensive screening should corroborate the initial findings of protein interactions via the SH2/SH3 domains. Ultimately, inhibition of the interaction of proteins with SH2/SH3 could reduce L-plastin phosphorylation and affect NSZ formation and bone resorption in conditions that display osteoclast activation and bone loss.
Inflammasomes in the Pathophysiology of Aortic Disease
Authors: Wortmann Markus , Peters Andreas S. , Erhart Philipp , Körfer Daniel , Böckler Dittmar , Dihlmann Susanne
Published: September 2021

Aortic diseases comprise aneurysms, dissections, and several other pathologies. In general, aging is associated with a slow but progressive dilation of the aorta, along with increased stiffness and pulse pressure. The progression of aortic disease is characterized by subclinical development or acute presentation. Recent evidence suggests that inflammation participates causally in different clinical manifestations of aortic diseases. As of yet, diagnostic imaging and surveillance is mainly based on ultrasonography, computed tomography (CT), and magnetic resonance imaging (MRI). Little medical therapy is available so far to prevent or treat the majority of aortic diseases. Endovascular therapy by the introduction of covered stentgrafts provides the main treatment option, although open surgery and implantation of synthetic grafts remain necessary in many situations. Because of the risks associated with surgery, there is a need for identification of pharmaceutical targets interfering with the pathophysiology of aortic remodeling. The participation of innate immunity and inflammasome activation in different cell types is common in aortic diseases. This review will thus focus on inflammasome activities in vascular cells of different chronic and acute aortic diseases and discuss their role in development and progression. We will also identify research gaps and suggest promising therapeutic targets, which may be used for future medical interventions.
Production of Green Biorefinery Protein Concentrate Derived from Perennial Ryegrass as an Alternative Feed for Pigs
Authors: Ravindran Rajeev , Koopmans Sybrandus , Sanders Johan P. M. , McMahon Helena , Gaffey James
Published: September 2021

Perennial rye grass is a widely used forage species in Ireland, on which the ruminant sector of agriculture is heavily dependent. While this species of grass is the primary source of fodder for cows, it is also abundant in plant protein, which could form a potential alternative ingredient in monogastric animal feed using a green biorefinery approach. In this study, perennial rye grass was processed using a novel biorefining process to extract value added products including protein as a potential replacement for soybean meal in monogastric feeds. Feed trials were conducted on a commercial farm with 55 weaner pigs for 31 days until slaughter. The diets comprised a control and a trial diet which integrated the green biorefinery protein concentrate. The effects of the new diet were determined by measuring the daily feed intake (DFI), average weight gain (AWG) and feed conversion ratio (FCR). Amino acid profiles of grass protein concentrate and soybean meal were comparable, with the latter having a slightly higher amount of total protein content, lysine and cysteine. The DFI and ADW indicated that the treatment diet was superior to the control. DFI for the treatment diet (1.512 kg/d) was 8% higher than the control diet (1.400 kg/d) by the end of the trial. Additionally, the ADW for the treatment diet was 6.44% higher than that achieved in the control sample. Meanwhile, FCR calculations indicated that the treatment diet is just as efficient as the conventional diet. Overall, the results of the study indicate positive potential for perennial ryegrass-derived green biorefinery protein concentrate as an alternative protein source for pig feed formulations in Ireland.
Influence of Reaction pH towards the Physicochemical Characteristics of Phosphorylated Polyvinyl Alcohol-Aluminum Phosphate Nanocomposite
Authors: Mohamed Saat Asmalina , Kamil Md Salim , Hamizi Nor Aliya , Badruddin Irfan Anjum , Ghazali Nadiah , Sagadevan Suresh , Kamangar Sarfaraz , Khan T. M. Yunus , Johan Mohd Rafie
Published: September 2021

The present study deals with the formation of a phosphorylated polyvinyl alcohol (PPVA)-Aluminum Phosphate (AlPO4) nanocomposite, changing the pH solution under the two-step process involving the phosphorylation of polyvinyl alcohol (PVA) followed by the conjugation with AlPO4. The composite was formed by varying the pH of the solution in the range of 7–12 and the reflected changes in the product’s morphology, crystallinity, surface nature, thermal stability, etc. were recorded using FESEM, XRD, FTIR, UV-Vis spectroscopy, TGA, etc. From the analysis, it was found that the particles formed with two different sizes of the probed pH, and at pH 10 they were homogeneously distributed. In addition, the morphology of the PPVA-AlPO4 composite also seems to be altered with respect to the pH and this is due to the differences in the amount of H+ and OH− anions. Thus, from the overall analysis, it can be indicated that pH 10 needs to be maintained for the formation of a spherical shape and uniformly distributed PPVA-AlPO4 nanocomposite.
Assessment of Stone Protective Coatings with a Novel Eco-Friendly Encapsulated Biocide
Authors: Zuena Martina , Ruggiero Ludovica , Caneva Giulia , Bartoli Flavia , Ventura Giancarlo Della , Ricci Maria Antonietta , Sodo Armida
Published: September 2021

The conservation of stone monuments is a constant concern due to their continuous weathering, in which biofouling plays a relevant role. To enhance the effectiveness of biocidal treatments and to avoid environmental issues related to their possible toxicity, this research aims at formulating and characterizing a coating charged with an eco-friendly biocide and showing hydrophobic properties. For this purpose, zosteric sodium salt—a natural biocide product—has been encapsulated into two silica nanocontainers and dispersed into a tetraethoxysilane-based (TEOS) coating also containing TiO2 nanoparticles. The coatings were applied on four different types of stone: brick, mortar, travertine, and Carrara marble. The effectiveness of the coating formulations and their compatibility concerning the properties of coated stones were assessed. The results showed that all coatings conferred a hydrophobic character to the substrate, as demonstrated by the increase of the static contact angle and the reduction in the capillary water absorption coefficient. The transmission of water vapor of the natural stones was preserved as well as their natural aspect. Furthermore, the coatings were homogeneously distributed on the surface and crack-free. Therefore, the protective capability of the coatings was successfully demonstrated.
Expression of Concern: Hamza et al. Efficacy of Mesenchymal Stem Cell and Vitamin D in the Treatment of Diabetes Mellitus Induced in a Rat Model: Pancreatic Tissues. Coatings 2021, 11, 317
Authors:   Coatings Editorial Office
Published: September 2021

Following acceptance of the Special Issue article by the Guest Editor, El-Sayed Abd El-Aziz, concerns were raised regarding the integrity of the peer review process [...]
Expression of Concern: Refat et al. Antioxidant, Antigenotoxic, and Hepatic Ameliorative Effects of Quercetin/Zinc Complex on Cadmium-Induced Hepatotoxicity and Alterations in Hepatic Tissue Structure. Coatings 2021, 11, 501
Authors:   Coatings Editorial Office
Published: September 2021

Following acceptance of the Special Issue article by the Guest Editor, El-Sayed Abd El-Aziz, concerns were raised regarding the integrity of the peer review process [...]
Expression of Concern: El-Megharbel et al. Utilizing of (Zinc Oxide Nano-Spray) for Disinfection against “SARS-CoV-2” and Testing Its Biological Effectiveness on Some Biochemical Parameters during (COVID-19 Pandemic)—“ZnO Nanoparticles Have Antiviral Activity against (SARS-CoV-2)”. Coatings 2021, 11, 388
Authors:   Coatings Editorial Office
Published: September 2021

Following acceptance of the Special Issue article by the Guest Editor, El-Sayed Abd El-Aziz, concerns were raised regarding the integrity of the peer review process [...]
Expression of Concern: Al-Thubaiti et al. Safety of Commercially Available Beef Burger in Saudi Arabia. Coatings 2021, 11, 686
Authors:   Coatings Editorial Office
Published: September 2021

Following acceptance of the Special Issue article by the Guest Editor, El-Sayed Abd El-Aziz, concerns were raised regarding the integrity of the peer review process [...]
Expression of Concern: Hamza et al. Therapeutic and Ameliorative Effects of Active Compounds of Combretum molle in the Treatment and Relief from Wounds in a Diabetes Mellitus Experimental Model. Coatings 2021, 11, 324
Authors:   Coatings Editorial Office
Published: September 2021

Following acceptance of the Special Issue article by the Guest Editor, El-Sayed Abd El-Aziz, concerns were raised regarding the integrity of the peer review process [...]
Improved Adsorption of the Antimicrobial Agent Poly (Hexamethylene) Biguanide on Ti-Al-V Alloys by NaOH Treatment and Impact of Mass Coverage and Contamination on Cytocompatibility
Authors: Zwicker Paula , Geist Norman , Göbler Elisabeth , Kulke Martin , Schmidt Thomas , Hornschuh Melanie , Lembke Ulrich , Prinz Cornelia , Delcea Mihaela , Kramer Axel , Müller Gerald
Published: September 2021

Unlike the native surface of the implant material (Ti6Al4V), oxidation with H2O2 leads to increased binding of the effective antimicrobial agent poly(hexamethylene) biguanide [PHMB]. However, treating with NaOH instead results in an even higher PHMB mass coverage. After oxidation with H2O2, strong differences in the PHMB adsorption capability between polished and corundum-blasted surfaces appear, indicating a roughness dependence. After NaOH treatment, no such effect was observed. The wetting properties of specimens treated with either H2O2 or NaOH prior to PHMB exposure clearly varied. To unravel the nature of this interaction, widespread in silico and in vitro experiments were performed. Methods: By X-ray photoelectron spectroscopy, scanning electron microscopy, water contact angle measurements and MD simulations, we characterized the interplay between the polycationic antimicrobial agent and the implant surface. A theoretical model for PHMB micelles is tested for its wetting properties and compared to carbon contaminated TiO2. In addition, quantitation of anionic functional group equivalents, the binding properties of PHMB with blocked amino end-group, and the ability to bind chlorhexidine digluconate (CHG) were investigated. Ultimately, the capability of osteoblasts to build calcium apatite, and the activity of alkaline phosphatase on PHMB coated specimens, were determined. Results: Simulated water contact angles on carbon contaminated TiO2 surfaces and PHMB micelle models reveal little influence of PHMB on the wetting properties and point out the major influence of remaining and recovering contamination from ambient air. Testing PHMB adsorption beyond the critical micelle concentration and subsequent staining reveals an island-like pattern with H2O2 as compared to an evenly modified surface with NaOH. Both CHG and PHMB, with blocked amino end groups, were adsorbed on the treated surfaces, thus negating the significant influence of PHMB’s terminal groups. The ability of osteoblasts to produce calcium apatite and alkaline phosphatase is not negatively impaired for PHMB mass coverages up to 8 μg/specimen. Conclusion: Differences in PHMB adsorption are triggered by the number of anionic groups and carbon contaminants, both of which depend on the specimen pre-treatment. With more PHMB covering, the implant surface is protected against the capture of new contamination from the ambient air, thus building a robust antimicrobial and biocompatible surface coating.
Effects of Surface Pretreatment of Titanium Substrates on Properties of Electrophoretically Deposited Biopolymer Chitosan/Eudragit E 100 Coatings
Authors: Pawłowski Łukasz , Bartmański Michał , Mielewczyk-Gryń Aleksandra , Zieliński Andrzej
Published: September 2021

The preparation of the metal surface before coating application is fundamental in determining the properties of the coatings, particularly the roughness, adhesion, and corrosion resistance. In this work, chitosan/Eudragit E 100 (chit/EE100) were fabricated by electrophoretic deposition (EPD) and both their microstructure and properties were investigated. The present research is aimed at characterizing the effects of the surface pretreatment of titanium substrate, applied deposition voltage, and time on physical, mechanical, and electrochemical properties of coatings. The coating’s microstructure, topography, thickness, wettability, adhesion, and corrosion behavior were examined. The applied process parameters influenced the morphology of the coatings, which affected their properties. Coatings with the best properties, i.e., uniformity, proper thickness and roughness, hydrophilicity, highest adhesion to the substrate, and corrosion resistance, were obtained after deposition of chit/EE100 coating on nanotubular oxide layers produced by previous electrochemical oxidation.
Synthesis and Spectroscopy of Buckminsterfullerene Cation C60+ in a Cryogenic Ion Trapping Instrument
Authors: Campbell Ewen K. , Rademacher Johanna , Bana Saida M. M.
Published: September 2021

The assignment of several diffuse interstellar bands in the near-infrared to C60+ ions present at high abundance in space has renewed interest in the astrochemical importance of fullerenes and analogues. Many of the latter have not been produced in macroscopic quantities, and their spectroscopic properties are not available for comparison with astronomical observations. An apparatus has been constructed that combines laser vaporisation synthesis with spectroscopic characterisation at low temperature in a cryogenic trap. This instrument is used here to record the electronic absorptions of C60+ produced by laser vaporisation of graphite. These are detected by (helium tagged) messenger spectroscopy in a cryogenic trap. By comparison with spectra obtained using a sublimed sample of Buckminsterfullerene, the observed data show that this isomer is the dominant C60+ structure tagged with helium at m/z=724, indicating that the adopted approach can be used to access the spectra of other fullerenes and derivatives of astrochemical interest.
Theoretical and Experimental Study of a Thermo-Mechanical Model of a Shape Memory Alloy Actuator Considering Minor Hystereses
Authors: Mitrev Rosen , Todorov Todor , Fursov Andrei , Ganev Borislav
Published: September 2021

The paper presents a theoretical and experimental investigation of a thermo-mechanical model of an actuator composed of a shape memory alloy wire arranged in series with a bias spring. The developed mathematical model considers the dynamics of the actuator in the thermal and mechanical domains. The modelling accuracy is increased through the developed algorithm for modelling the minor and sub minor hystereses, thus removing the disadvantages of the classical model. The algorithm improves the accuracy, especially when using pulse-width modulation control, for which minor and sub minor hystereses are likely to occur. Experimental studies show that the system is very sensitive, and there are physical factors whose presence cannot be considered in the mathematical model. The experimental research has shown that setting constant values of the duty cycle is impossible to obtain a stable value of displacement and force. The comparison between the developed mathematical model results and the experimental results shows that the differences are acceptable. The improved modelling serves as a basis for designing such actuators and creating an improved automatic feedback control system to maintain a given displacement (force) or trajectory tracking.
Structural, Optical, and Photocatalytic Properties of ZnSe Nanoparticles Influenced by the Milling Time
Authors: Hien Bui Thi Thu , Mai Vu Thanh , Thuy Pham Thi , Hoa Vu Xuan , Chi Tran Thi Kim
Published: September 2021

ZnSe nanoparticles (NPs) were prepared by combining both hydrothermal and mechanical milling methods. Transmission electron microscopy images show that fabricated ZnSe NPs with a sphere-like shape have an average size (d) in the range of 20–100 nm, affected by changing the milling time from 10 to 60 min. All the samples crystalize in zincblende-type structure without impurities, as confirmed by analyzing X-ray diffraction patterns, Raman spectra, and energy-dispersive X-ray spectroscopy. Carefully checking Raman spectra, we have observed the broadening and redshift of vibration modes as decreasing NP size, which are ascribed to extra appearance of disorder and defects. The photoluminescence study has found a blue emission at 462 nm attributed to the excitonic near-band edge and a broad defect-related emission around 520–555 nm. Increasing milling time leads to the decrease in the exciton-emission intensity, while the defect-related emissions increase gradually. Interestingly, as decreasing d, we have observed an improved photodegradation of Rhodamine B under UV irradiation, proving application potentials of ZnSe NPs in photocatalytic activity.
Comprehensive Proteomic Analysis of Colon Cancer Tissue Revealed the Reason for the Worse Prognosis of Right-Sided Colon Cancer and Mucinous Colon Cancer at the Protein Level
Authors: Chen Yanyu , Hou Wenyun , Zhong Miner , Wu Bin
Published: September 2021

To clarify the molecular mechanisms underlying the poor prognosis of right-sided and mucinous colon cancer at the proteomic level. A tandem mass tag-proteomics approach was used to identify differentially expressed proteins (DEPs) in colon carcinoma tissues from different locations and with different histological types to reveal the underlying mechanisms of these differences at the protein level. In additional, the DEPs were analyzed using bioinformatics methods. The proteomics profiles among colon cancers with different tumor locations and histological types were dramatically distinguished. In terms of tumor locations, the right-sided carcinoma specific DEPs may promote the tumor progression via activating inflammation, metastasis associated pathways. When referring to histological types, the mucinous colon cancers perhaps increased the invasion and metastasis through distinct mechanisms in different tumor locations. For mucinous cancer located in right-sided colon, the mucinous specific DEPs were mainly associated with ECM-related remodeling and the IL-17 signal pathway. For mucinous cancer located in left-sided colon, the mucinous specific DEPs showed a strong relationship with ACE2/Ang-(1–7)/MasR axis. The proteomics profiles of colon cancers showed distinct differences related to locations and histological types. These results suggested a distinct mechanism underlying the diverse subtypes of colon cancers.
Comparative Evaluation of Bruker Biotyper and ASTA MicroIDSys for Species Identification in a Clinical Microbiology Laboratory
Authors: Chung Yousun , Han Minje , Kim Jae-Seok
Published: September 2021

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI–TOF MS) has been widely used for microbial identification, because of its speed and accuracy, since its introduction to clinical microbiology laboratories. In this study, we evaluated the performance of ASTA MicroIDSys, a newly developed MALDI–TOF, and compared it with the widely used Bruker Biotyper. Microbial identification with the Bruker Biotyper system was performed by using a direct smear method and the Bruker Biotyper database (reference library version 6.0.0.0). The isolates were also tested in parallel, using the ASTA MicroIDSys system with a direct smear method and the MicroIDSys database, CoreDB v1.26. A total of 914 clinical isolates were recovered from the clinical specimens. Identical results with confidence scores (≥2.0, for the Bruker Biotyper) and acceptable scores (≥140 for the ASTA MicroIDSys) were obtained for 840 (91.9%) isolates. The minor errors were defined as misidentification at the species level, and the rate was 1.1% (9/792) for Bruker Biotyper and 1.6% (13/792) for ASTA MicroIDSys. Major errors were defined as misidentification at the genus level, and the rate was 0.3% (2/792) for both Bruker Biotyper and ASTA MicroIDSys. ASTA MicroIDSys showed reliable performance for microbial identification, which was comparable to that of the Bruker Biotyper. Therefore, ASTA MicroIDSys can be applied for the identification of microorganisms in clinical microbiology laboratories.
Geometric Characteristics of the Wasserstein Metric on SPD(n) and Its Applications on Data Processing
Authors: Luo Yihao , Zhang Shiqiang , Cao Yueqi , Sun Huafei
Published: September 2021

The Wasserstein distance, especially among symmetric positive-definite matrices, has broad and deep influences on the development of artificial intelligence (AI) and other branches of computer science. In this paper, by involving the Wasserstein metric on SPD(n), we obtain computationally feasible expressions for some geometric quantities, including geodesics, exponential maps, the Riemannian connection, Jacobi fields and curvatures, particularly the scalar curvature. Furthermore, we discuss the behavior of geodesics and prove that the manifold is globally geodesic convex. Finally, we design algorithms for point cloud denoising and edge detecting of a polluted image based on the Wasserstein curvature on SPD(n). The experimental results show the efficiency and robustness of our curvature-based methods.
The Information Conveyed in a SPAC′s Offering
Authors: Cohen Gil , Qadan Mahmoud
Published: September 2021

The popularity of SPACs (Special Purpose Acquisition Companies) has grown dramatically in recent years as a substitute for the traditional IPO (Initial Public Offer). We modeled the average annual return for SPAC investors and found that this financial tool produced an annual return of 17.3%. We then constructed an information model that examined a SPAC′s excess returns during the 60 days after a potential merger or acquisition had been announced. We found that the announcement had a major impact on the SPAC’s share price over the 60 days, delivering on average 0.69% daily excess returns over the IPO portfolio and 31.6% cumulative excess returns for the entire period. Relative to IPOs, the cumulative excess returns of SPACs rose dramatically in the next few days after the potential merger or acquisition announcement until the 26th day. They then declined but rose again until the 48th day after the announcement. Finally, the SPAC’s structure reduced the investors’ risk. Thus, if investors buy a SPAC stock immediately after a potential merger or acquisition has been announced and hold it for 48 days, they can reap substantial short-term returns.
Teaching Congruences in Connection with Diophantine Equations
Authors: Viliam Ďuriš , Dalibor Gonda , Anna Tirpáková , Pavlovičová Gabriela
Published: September 2021

The presented paper is devoted to the new teaching model of congruences of computer science students within the subject of discrete mathematics at universities. The main goal was to create a new model of teaching congruences on the basis of their connection with Diophantine equations and subsequently to verify the effectiveness and efficiency of the proposed model experimentally. The teaching of congruences was realized in two phases: acquisition of procedural knowledge and use of Diophantine equations to obtain conceptual knowledge of congruences. Experiments confirmed that conceptual understanding of congruences is positively related to increasing the procedural fluidity of congruence resolution. Research also demonstrated the suitability of using Diophantine equations to link congruences and equations. Among other things, the presented research has confirmed the justification of teaching mathematics in computer-oriented study programs.
The PRU: The Solution for Whom?
Authors: Malmqvist Johan
Published: September 2021

In Sweden, pupil referral units (PRUs) have been recommended by the government, suggesting that “inclusion has gone too far”. This governmental recommendation is not based on research focusing on PRUs, as such research is sparse. Furthermore, there has been a lack of evaluations of the efficacy of PRUs, and no national evaluations of such provision have been undertaken. Furthermore, more attention must be paid to PRU students’ own perspectives and experiences as we lack knowledge of their needs and situation. This study aimed to investigate how educational needs have been and should be addressed in one PRU according to nine stakeholder groups, for example, current students, former students, parents, school staff, and various groups of people who, in their work, were responsible for deciding about the PRU (e.g., chief education officers or politicians) or supporting the PRU (e.g., school healthcare unit staff). Comparative analysis of all groups’ perceptions considered similarities and differences of views of this topic. Preliminary results indicate substantial between- and within-group variation concerning the purpose of the PRU and uncertainty about educational quality, partly due to insufficient documentation. Some students described a “Catch-22”: having been told to catch up educationally with peers and that PRU placement would help in this, they were disappointed, as the emphasis on non-educational practices impeded catching up.
Combined Full-Reference Image Quality Metrics for Objective Assessment of Multiply Distorted Images
Authors: Okarma Krzysztof , Lech Piotr , Lukin Vladimir V.
Published: September 2021

In the recent years, many objective image quality assessment methods have been proposed by different researchers, leading to a significant increase in their correlation with subjective quality evaluations. Although many recently proposed image quality assessment methods, particularly full-reference metrics, are in some cases highly correlated with the perception of individual distortions, there is still a need for their verification and adjustment for the case when images are affected by multiple distortions. Since one of the possible approaches is the application of combined metrics, their analysis and optimization are discussed in this paper. Two approaches to metrics’ combination have been analyzed that are based on the weighted product and the proposed weighted sum with additional exponential weights. The validation of the proposed approach, carried out using four currently available image datasets, containing multiply distorted images together with the gathered subjective quality scores, indicates a meaningful increase of correlations of the optimized combined metrics with subjective opinions for all datasets.