bluuebunny/crossref_metadata_2025 huggingface dataset:
Application of indirect observation within mixed methods to contrast the results obtained through direct observation: a discussion with observed Pikler educators
Authors: Belza Haizea , Sagastui Jone , Herrán Elena
Published: June 2024

Introduction

This work arises from a previous research, “Pikler educators early in the morning” carried out in the Emmi Pikler Nursery School in Budapest through Systematic Observation. In it, Piklerian choreographies were found in observed educators’ behavior during the studied three daily activities: feeding breakfast, dressing to go to the garden and free play accompanying. All of them share certain Piklerian principles, which are synthesized in three central keys: the stability of the educator’s behavior, her strategic and intentional positioning, and an active emotional control.

Objectives

This study aims to contrast this synthesis of results by means of an in-depth interview with the two observed educators, and to apply the methodological approach of indirect observation within mixed methods for its analysis. The objective is to confirm whether the three central keys are recognized as their own and to look for new theoretical-practical elements within the studied educational approach.

Materials and methods

We applied an in-depth interview and analyzed it following the guidelines of indirect observation. The participants were the two educators previously observed, a translator from the Pikler team, and the three observers, authors of this work. An ad hoc observation instrument was elaborated, and the three macro-stages QUAL-QUAL-QUAL proposed within mixed methods were rigorously followed.

Results

Lag sequential analysis was used to conduct data analyses. We deepened in prospective lags and obtained the response pattern underlying the interview. Then, we performed a concurrence analysis to investigate the relationship between the central keys obtained in our original research and Piklerian ideas.

Conclusion

In-depth interview within mixed methods has been a novel and generous tool leading us to substantial and methodological contributions, despite the simplicity of performed analyses. Interviewed educators’ response pattern is a faithful reflection of the Piklerian modus operandi. The study of concurrences shows that Piklerian education is something natural, integrated in its professionals, with the exception of emotional control, which still requires permanent reflection.
The central role of mindful parenting in child’s emotional regulation and human flourishing: a blueprint perspective
Authors: Sansone Antonella
Published: June 2024

This article provides an innovative perspective of emotional-regulation and human flourishing which acknowledges the fundamental role of early parent–child experiences in shaping brain structure and functioning involved in emotional regulation and the central role of mindful parenting in facilitating emotional regulation in both parent and child (co-regulation). In this perspective paper the author underlines not only the central role of emotions and emotional regulation in human development and flourishing, but also the importance of maternal mental health, mindfulness, and a connected supportive community during pregnancy and postnatally in facilitating emotional regulation in both the caregiver and the infant and thus promoting secure attachment. The role of alloparenting and how we evolved to share childrearing is introduced, and emotional regulation is described not as an individual phenomenon but a relational embodied process. The associations between right brain functioning, mindfulness and secure attachment, all leading to emotional regulation, wellbeing, and resilience are described. Sharing findings and perspectives offer an opportunity for insights and reflection upon what strategies could be created to promote relational emotional regulation and wellbeing in early life, thus human flourishing leading to a peaceful society.
Oral discomfort and health behavior of patients with typical vs. atypical antipsychotic drugs
Authors: Meurman Jukka H. , Murtomaa Heikki
Published: June 2024

Introduction

Psychiatric patients suffer from oral diseases and side effects of antipsychotic medication. In particular, the typical antipsychotic drugs may cause severe hyposalivation with subsequent oral symptoms. We therefore aimed to compare oral health behavior and oral side effects of in-hospital patients taking typical vs. atypical antipsychotic drugs with the hypothesis that the former drugs cause more oral pain than the newer drugs.

Methods

This cross-sectional questionnaire and interview study investigated subjective oral symptoms and their health behavior in 170 hospitalized psychiatric patients, comparing those taking typical vs. atypical antipsychotic drugs. Cross-tabulations and chi-square tests were used for analyses.

Results

Persistent oral pain lasting throughout the day was reported by 46% in the typical, and 5% in the atypical antipsychotic group patients, respectively. In both groups, the pain was mainly in the tongue and buccal mucosa and was described as a burning sensation. A significantly higher prevalence of xerostomia was reported in the typical antipsychotic medication group (66%) compared with the atypical antipsychotic medication group (53%, p<0.01). Self-assessed dental health was assessed as poor by two-thirds of the patients of whom 69% reported toothbrushing once daily. Approximately half of them reported having had a visit to a dentist within the previous year. Of the women 28%, and of the men 17%, respectively, had received professional consultations for oral symptoms.

Conclusion

The current results on psychiatrically hospitalized patients emphasize the need for awareness of oral discomfort and its subsequent effects on the quality of life in this challenging patient group. Focus should also be placed on a wide range of support encouraging the patients to maintain good daily oral hygiene and seek professional dental help when needed.
The relationship between attachment, primary emotions and positive/negative spirituality: a path analysis
Authors: Freund Anton , Fuchshuber Jürgen , Silani Giorgia , Unterrainer Human-Friedrich
Published: June 2024

Objective

The present study investigates what may influence individuals to experience their religiosity/spirituality as either subjectively positive [religious or spiritual (r/s) wellbeing] or as negative (r/s struggles). Drawing on existing literature attachment insecurity and the seven primary emotions as outlined by Jaak Panksepp in Affective Neuroscience are identified as likely influences.

Methods

The final sample consisted of 340 participants (age: M = 36, SD = 14.2; 68.5% = female), among which 65% self-identified as religious/spiritual. A path analysis was conducted to test a proposed mediation model in which the expected effects of primary emotions (B-ANPS) on r/s wellbeing (MI-RSWB) and r/s struggles (RSSS) were mediated through attachment insecurity (ECR-RD8).

Results

The data indicated that attachment insecurity fully mediated the relationships between the primary emotions SADNESS and LUST with r/s struggles. Furthermore, the primary emotions FEAR and ANGER displayed small direct effects on both r/s struggles and r/s wellbeing. Overall, the model, which demonstrated excellent model fit, was able to explain 30% of the variance of r/s struggles, 24% of attachment insecurity and 5% of r/s wellbeing.

Conclusions

The findings suggest that primary emotions such as SADNESS and LUST substantially explain r/s struggles and that these relationships seem to be mediated through attachment. Moreover, r/s struggles seem to be qualitatively distinct from r/s wellbeing. Finally, a moderate link between LUST and attachment suggests that sexuality plays a significant role in (adult) attachment processes.
Job satisfaction and social identification among paramedics in southern Poland
Authors: Kukla Paweł , Kózka Maria , Siemiginowska Patrycja , Ilczak Tomasz , Augustyn Magdalena , Malinowska-Lipień Iwona
Published: June 2024

Introduction

Job satisfaction, based on professional and non-professional factors and individual characteristics of employees, is an important element influencing both the quality of care provided and employee turnover.

Material and method

The study included 137 paramedics employed in field teams and hospital emergency departments. The Job Satisfaction Scale (SSP), the Minnesota Job Satisfaction Questionnaire (MSQ), the Utrecht Work Engagement Scale (UWES), and the Three Dimensional Strength of Group Identification Scale (TSIG) were used to collect the data.

Results

The average job satisfaction score measured with SSP in the studied group of paramedics was 24.50 and the average job satisfaction score measured with MSQ was 74.16. The average value of the group identification in the study sample was 61.15. Of the three subscales, the highest scores were obtained in the affect toward the group subscale −22.44, and the lowest in the cognitive centrality subscale −18.78. The analysis showed that job satisfaction positively correlated with social identification (r = 0.43) and the ingroup ties (r = 0.43), cognitive centrality (r = 0.34) and ingroup affect (r = 0.37).

Conclusions

The studied group of paramedics showed moderate job satisfaction (measured with SSP) and work engagement, with a simultaneous high level of job satisfaction (measured with MSQ) and social identification with the professional group. Social identification of studied paramedics varied depending on gender. Women showed higher levels of cognitive centrality, which might mean that they might have had greater need to categorize themselves as paramedics.
Exploring twitter discussions on healthy food: inspiring, access to healthy food for young, and nutritional economics in focus
Authors: Kvasnička Roman , Kvasničková Stanislavská Lucie , Pilař Ladislav , Kuralová Kateřina , Pilařová Lucie , Čejka Martin
Published: June 2024

Considering that food is a critical factor for a healthy population, social media analysis of discussions about this area is very important to understanding people’s behavior. This research aimed to identify the main areas of communication about healthy food, including sentiment identification, on the social network Twitter (now X). We achieved this aim by analyzing 828,963 Tweets posted from 1 April 2018 to 17 April 2023 based on a dataset that included all Tweets containing the hashtag “#healthyfood” or the phrase “healthy food.” This study extends previous research that focused only on hashtags and did not include all messages about healthy food. The results identified five main communication areas, namely (1) Inspiring, (2) Access to healthy food for children and youth, (3) Expenditure on healthy food, (4) Homemade food, and (5) Vegan/vegetarian diet, of which the second and third areas were not identified in previous research due to selective analysis focusing only on hashtags. These two communities had the highest negative sentiment of the top 10 communities, suggesting that these issues require attention.
Willingness to vaccinate and willingness to pay for vaccination against peste des petits ruminants in northern Senegal
Authors: Ilboudo Guy Sidwatta , Wanyoike Francis , Bahta Sirak , Sy Samba , Djigo Cheikh Ahmed Tidiane , Sall Papa Anoune , Lô Mamadou Moustapha , Dione Michel
Published: June 2024

Background

This study was carried out in Linguere department, Louga region of Senegal. Its objective was to explore the socio-economic factors that influence small ruminant producers’ decisions on vaccination against peste des petits ruminants.

Methods

We carried out a willingness to vaccinate and willingness to pay for vaccination using a choice experiment approach with 200 small ruminant producers.

Results

Results showed that the key factors that influence willingness to vaccinate, include perceived benefits of vaccination (98, 95%CI: 96–100%), the type of vaccinator (91, 95%CI: 87–95%), the access to information (86, 95%CI: 81–91%), the vaccine availability (80, 95%CI: 74–86%), and the possession of a vaccination certificate by the producer (76, 95%CI: 70–82%). Preferences of producers leaned toward home vaccination (preference weight = 0.74, p = 1%), individual herd vaccination (preference weight = 0.45, p = 1%), elective participation to vaccination (preference weight = 0.33, p = 0.01), and low-cost services (preference weight = −0.004, p = 0.1). Producers expressed a willingness to pay for vaccination per animal of XOF 184 (USD 0.3), XOF 113 (USD 0.18), and XOF 82 (USD 0.13) for home, individual herd, and elective vaccination, respectively.

Conclusion

The findings underscore the importance of targeted awareness campaigns and bringing vaccination services closer to the producers.
An injectable subunit vaccine containing Elongation Factor Tu and Heat Shock Protein 70 partially protects American bison from Mycoplasma bovis infection
Authors: Kaplan Bryan S. , Dassanayake Rohana P. , Briggs Robert E. , Kanipe Carly R. , Boggiatto Paola M. , Crawford Lauren S. , Olsen Steven C. , Menghwar Harish , Casas Eduardo , Tatum Fred M.
Published: June 2024

Mycoplasma bovis (M. bovis) is the etiologic agent of high mortality epizootics of chronic respiratory disease in American bison (Bison bison). Despite the severity of the disease, no efficacious commercial vaccines have been licensed for the prevention of M. bovis infection in bison. Elongation factor thermal unstable (EFTu) and Heat Shock Protein 70 (Hsp70, DnaK) are highly conserved, constitutively expressed proteins that have previously been shown to provide protection against M. bovis infection in cattle. To assess the suitability of EFTu and Hsp70 as vaccine antigens in bison, the immune response to and protection conferred by an injectable, adjuvanted subunit vaccine comprised of recombinantly expressed EFTu and Hsp70 was evaluated. Vaccinates developed robust antibody and cellular immune responses against both EFTu and Hsp70 antigens. To assess vaccine efficacy, unvaccinated control and vaccinated bison were experimentally challenged with bovine herpes virus-1 (BHV-1) 4 days prior to intranasal infection with M. bovis. Vaccinated bison displayed reductions in joint infection, lung bacterial loads, and lung lesions compared to unvaccinated controls. Together, these results showed that this subunit vaccine reduced clinical disease and bacterial dissemination from the lungs in M. bovis challenged bison and support the further development of protein subunit vaccines against M. bovis for use in bison.
Optimization Design of Core Ultra-Stable Structure for Space Gravitational Wave Detection Satellite Based on Response Surface Methodology
Authors: Liu Changru , Xu Zhenbang , Han Kang , Han Chengshan , He Tao
Published: June 2024

In order to meet the urgent demand for novel zero-expansion materials and ultra-stable structures in space gravitational wave detection, it is necessary to develop an ultra-stable structural spacecraft system. This paper focuses on the research of the optimization of the core ultra-stable structure design of spacecraft, proposing a cross-scale parameterized model of structural deformation response and a multi-objective optimization method. By satisfying the prerequisites of mass and fundamental frequency, this paper breaks through the limitations of current linear analysis methods, and the overall thermal deformation of nonlinear material composite structures is optimized by modifying structural parameters.
Comparative Analysis of Drought Effects on the Soil Moisture Level and Penetration Resistance in Conventional and Non-Conventional Tillage Systems in Maize Production
Authors: Sojnóczki István , Nagy János , Illés Árpád , Kecskés István , Bojtor Csaba
Published: June 2024

The period of extreme weather anomalies in recent years has challenged farmers, and this has encouraged greater adaptability in farming practices. In the last decade, conventional tillage systems have been complemented by more biologically based cropping systems. The research evaluated the impact of drought on soil physical parameters in maize production by testing different conventional and non-conventional tillage systems to ensure optimal soil physical parameters. In the analysis of the prevailing weather conditions, rainfall values were divided into two parts, the pre-growing season and the growing season. We studied different climatic seasons. In 2021, the soil moisture content in the upper shallow 15 cm soil layer during the sowing period in April in the case of conventional tillage was significantly lower than in reduced tillage, conservation tillage and strip tillage. The most significant difference was measured between conventional and conservation tillage, with a difference of 11.25 v/v%. The 2022 crop year was extremely dry. In June, the highest moisture value in the soil was measured in the case of strip tillage with a value of 21.64 v/v%, which was more than 60% higher than in the case of conventional and conservation tillage. In conventional tillage, a very pronounced compacted layer was observed in the lower part of the ploughed layer. This zone reached a compaction of 6.9 MPa between 28 and 34 cm, which is agronomically harmful. No compacted soil layer was found in the experiment site under conservation tillage. In the severe drought year of 2022, only the strip-till system provided the proper water management conditions for the maize stand.
Identification and Fine Mapping of Quantitative Trait Loci for Tiller Angle Using Chromosome Segment Substitution Lines in Rice (Oryza Sativa L.)
Authors: Leng Yujia , Tao Tao , Lu Shuai , Liu Ran , Yang Qingqing , Zhang Mingqiu , Hong Lianmin , Guo Qianqian , Ren Xinzhe , Yang Zhidi , Cai Xiuling , Jin Sukui , Gao Jiping
Published: June 2024

The tiller angle, which is an important agronomic trait, determines plant architecture and greatly influences the grain yield of rice. In this study, a population of chromosome segment substitution lines derived from a cross between a japonica variety with a compact plant architecture—Koshihikari—and an indica variety with a spread-out plant architecture—Nona Bokra—was used to investigate the genetic basis of the tiller angle. Five quantitative trait loci (qTA1, qTA5, qTA9-1, qTA9-2, and qTA11) for the tiller angle were detected on chromosomes 1, 5, 9, 9, and 11 in two different environments. The phenotypic variation in these QTLs ranged from 3.78% to 8.22%. Two pairs of digenic epistatic QTLs were detected in Lingshui. The epistatic interaction explained 15.19% and 13.60% of the phenotypic variance, respectively. Among the five QTLs, qTA9-2 was detected in both environments. An F2 mapping population containing the qTA9-2 QTL was established. The location of qTA9-2 was narrowed down to a 187 kb region between InDel markers M9 and M10 on chromosome 9. Thirty open reading frames (ORFs), including TAC1, a gene known to regulate the tiller angle, were identified in this region. The gene sequencing results suggested that a base substitution from G to A at position 1557 in the 3′-untranslated region led to a difference in the expression of qTA9-2 in Koshihikari and Nona Bokra. These findings provide a potential gene resource for the improvement of rice plant architecture.
Technical Efficiency and Allocative Efficiency of Beef Cattle Fattening in the Content of Digital Economy: An Empirical Study Based on Survey in China
Authors: Xue Yongjie , Qi Zhenhua , Yan Jinling , Li Dahai , Zhao Huifeng , Zheng Haijing
Published: June 2024

This study applies data envelopment analysis (DEA) to gauge technical efficiency and allocative efficiency in China’s beef cattle-fattening industry using survey data. The Tobit model considers the salient determinants that drive these efficiencies. The results indicate that (1) large-scale farms exhibit robust TE and pure technical efficiencies (PTE), whereas scale efficiencies (SE) diverge significantly between large and medium-sized operations. The cost efficiency (CE) of smaller farms lags behind their larger counterparts, with the latter displaying greater revenue efficiencies (RE) and profit efficiency (PE). (2) The influence of identical factors on the efficiency of beef cattle fattening production can vary, sometimes antithetically, across different scales. Local policy interventions must be differentiated according to farm type and size. (3) The unique context of China’s national conditions and the status quo of livestock farming render the dual implementation of environmental regulations and technological subsidies less viable for Chinese beef cattle farms. These entities should prioritize production over technological innovation and advancement. Policymakers should adopt strategies such as targeted skill/technological training for farm managers at particular scales of operation. This could represent a critical trajectory to augment the efficiency of beef cattle production and increase beef yield in China.
A Multidisciplinary Approach for the Development of a Supply Chain in Biomass Conversion of Agrifood Waste Mediated by Larvae of Hermetia illucens L.: From Rearing to By-Product Exploitation
Authors: De Santis Eleonora , de Iudicibus Alberto , Lecce Francesca , De Mei Massimiliano , Petrazzuolo Francesco , Del Giudice Angelo , Carnevale Monica , Gallucci Francesco , Beni Claudio , Assirelli Alberto , Santangelo Enrico , Arnone Silvia
Published: June 2024

Black soldier fly larvae (BSFL) can convert various organic substrates into high added-value biomass. In addition, the residue can be used as a soil conditioner. Several studies have been conducted on a laboratory scale that may not represent what happens on a prototype scale. Using fruit and vegetable waste as a basic substrate, mixing them with agro-industry by-products (called co-substrates), the Hermes project set up a process on medium (2 kg) and large (10 kg) scales with two different feeding regimes (1.25 g/BSFL and 2 g/BSFL). At the mature stage, larval biomass was separated from frass (the by-product of the larval rearing). The production of larval proteins and fats and the use of frass as soil conditioning were evaluated. The lowest feeding regime (1.25 g/BSFL) provided the best waste valorization. The shift towards higher production scales is not completely linear. The addition of co-substrates to fruit and vegetable waste, as they are provided by the large-scale retail trade, can help to standardize a process as part of an insect farm. The frass recovered from the residue of rearing (on the diet or on the agrifood leftovers) was composted and used in field to grow a processing tomato variety. The addition of composted frass assured a slightly lower yield than synthetic fertilizer but there was no statistically significant difference (p > 0.10). This suggests that partial replacement of synthetic fertilizer with composted frass has potential. Overall, the work demonstrated that, using a multidisciplinary approach, the interest and the value in building a supply chain based on bioconversion mediated by Hermetia illucens can be emphasized.
YOLOv8n-DDA-SAM: Accurate Cutting-Point Estimation for Robotic Cherry-Tomato Harvesting
Authors: Zhang Gengming , Cao Hao , Jin Yangwen , Zhong Yi , Zhao Anbang , Zou Xiangjun , Wang Hongjun
Published: June 2024

Accurately identifying cherry-tomato picking points and obtaining their coordinate locations is critical to the success of cherry-tomato picking robots. However, previous methods for semantic segmentation alone or combining object detection with traditional image processing have struggled to accurately determine the cherry-tomato picking point due to challenges such as leaves as well as targets that are too small. In this study, we propose a YOLOv8n-DDA-SAM model that adds a semantic segmentation branch to target detection to achieve the desired detection and compute the picking point. To be specific, YOLOv8n is used as the initial model, and a dynamic snake convolutional layer (DySnakeConv) that is more suitable for the detection of the stems of cherry-tomato is used in neck of the model. In addition, the dynamic large convolutional kernel attention mechanism adopted in backbone and the use of ADown convolution resulted in a better fusion of the stem features with the neck features and a certain decrease in the number of model parameters without loss of accuracy. Combined with semantic branch SAM, the mask of picking points is effectively obtained and then the accurate picking point is obtained by simple shape-centering calculation. As suggested by the experimental results, the proposed YOLOv8n-DDA-SAM model is significantly improved from previous models not only in detecting stems but also in obtaining stem’s masks. In the mAP@0.5 and F1-score, the YOLOv8n-DDA-SAM achieved 85.90% and 86.13% respectively. Compared with the original YOLOv8n, YOLOv7, RT-DETR-l and YOLOv9c, the mAP@0.5 has improved by 24.7%, 21.85%, 19.76%, 15.99% respectively. F1-score has increased by 16.34%, 12.11%, 10.09%, 8.07% respectively, and the number of parameters is only 6.37M. In the semantic segmentation branch, not only does it not need to produce relevant datasets, but also improved its mIOU by 11.43%, 6.94%, 5.53%, 4.22% and mAP@0.5 by 12.33%, 7.49%, 6.4%, 5.99% compared to Deeplabv3+, Mask2former, DDRNet and SAN respectively. In summary, the model can well satisfy the requirements of high-precision detection and provides a strategy for the detection system of the cherry-tomato.
New Frontiers in the Cultivation of Edible Fungi: The Application of Biostimulants Enhances the Nutritional Characteristics of Pleurotus eryngii (DC.) Quél
Authors: Fedeli Riccardo , Mazza Irene , Perini Claudia , Salerni Elena , Loppi Stefano
Published: June 2024

Fungi, particularly Pleurotus eryngii, emerges as a promising solution for sustainable non-animal protein production, requiring less land and growing on waste materials. In connection with population growth, sustainable solutions must be found to increase yield and product quality without resorting to the use of synthetic chemical fertilizers. Several biobased products are currently on the market; one of the most interesting is wood distillate (WD), derived from the pyrolysis process of the woody material. WD is rich in biologically active substances such as polyphenols, alcohols, acids, and esters, and its use is authorized in organic agriculture. The study investigates the use of WD in cultivating P. eryngii. We tested different concentrations of WD: 0%, 0.1%, 0.2%, 0.5%, and 1% WD on the growth of P. eryngii. Although WD did not significantly affect the yield (fresh weight), it led to a substantial increase in total soluble protein content and antioxidant compounds, such as phenols and vitamin C, and a reduction in glycogen content, especially at 0.2% WD. The results highlight the potential of biostimulants in mushroom cultivation, providing the ground for further research to improve the nutritional properties of cultivated mushrooms through wood distillate.
Reinoculation in Topdressing of Rhizobium tropici, Azospirillum brasilense, and the Micronutrients Mo/Co in Common Bean
Authors: Ribeiro Brenda B.A. , Teixeira Itamar R. , Silva Gisele C. , Bravo Tamires Ester P. , Cunha Nathan Mickael B. , Benício Neto Maurílio R. , Alves Gessiele P.C. , Sbroggio Filho Alexandre M. , Reis Elton F.
Published: June 2024

Biological nitrogen fixation (BNF) can provide the necessary nitrogen for bean crops; however, for this to occur, important limitations involving the inoculant application technology need to be overcome.The use of co-inoculation is a management technique used to obtain benefits and increase the potential of N2 fixation from the association between bacteria from the rhizobia group, such as R. tropici, and bacteria that promote plant growth, such as A. brasilense, in association with the addition of nutrients that allow greater efficiency of bacteria fixing atmospheric N2. This study aimed to evaluate the bean response to the reinoculation of R. tropici in co-inoculation with A. brasilense in a mixture with the micronutrients Co/Mo, in the winter season of 2021, in Anápolis-GO, Brazil. A randomized block design was used, with four replications, and the following treatments (TRs) were studied: TR1—reinoculation with R. tropici; TR2—reinoculation with co-inoculation of R. tropici + A. brasilense; TR3—reinoculation of R. tropici + Mo/Co micronutrients; TR4—reinoculation with co-inoculation R. tropici + A. brasilense + Mo/Co micronutrients; TR5—inoculation via seed, without reinoculation; TR6—mineral N fertilization in the sowing furrow and topdressing; TR7—control, without any N source. At stage R6, nodulation characteristics (number and dry mass of nodules) and the morphophysiological parameters of the plants (main root length, root dry mass, plant height, shoot dry mass, leaf area, and leaf N content in the shoot) were evaluated. At harvest, the final plant stand and components (number of pods per plant, number of grains per pod, and average weight of one hundred grains) were determined, in addition to grain yield. It was concluded that inoculation followed by reinoculation in topdressing with R. tropici in co-inoculation with A. brasilense plus Mo/Co, compared to mineral nitrogen fertilization, improves the efficiency of the nodulation process and the morphophysiological characteristics of the common bean crop. Seed inoculation and topdressing application with R. tropici, associated with co-inoculation with A. brasilense + Mo and Co, have the potential to completely replace mineral nitrogen fertilization in common bean crops.
The Sugarcane ScPetC Gene Improves Water-Deficit and Oxidative Stress Tolerance in Transgenic Tobacco Plants
Authors: Silva Carolina Ribeiro Liberato , de Souza César Bueno , dos Santos Claudiana Moura , Floreste Bruno Spinassé , Zani Nicholas Camargo , Hoshino-Bezerra Andrea Akemi , Bueno Giane Carolina , Chagas Eder Bedani Ruiz , Menossi Marcelo
Published: June 2024

Water deficit is the main limiting factor constraining sugarcane productivity, and its impact is expected to increase due to climate changes. During prolonged drought periods, most plants become extremely vulnerable to ROS accumulation, which can severely damage their photosynthetic apparatus. The PetC gene, encoding a Rieske FeS protein (ISP), has been shown to regulate the electron transport chain and protect photosystems (PSs) under drought conditions in some plant species. In sugarcane, transcriptome analysis revealed that ScPetC is repressed during drought stress in the field. In this study, we have overexpressed ScPetC in tobacco plants and evaluated its role in water-deficit tolerance. Our results indicate that the ScPetC protein structure is conserved when compared to other species. ScPetC overexpression reduced the negative impact of water deficit on plant development. This effect was associated with a positive impact on ScPetC quantum efficiency and the electron transport rate of PSII, the photosynthetic rate, and water use efficiency. The total chlorophyll content under water deficit was higher in plants overexpressing ScPetC, and this was correlated with less chlorophyll degradation from oxidative damage. Together, these results demonstrate that ScPetC confers tolerance to water deficit and oxidative stresses, making it a candidate gene for crop improvement.
A Complex Approach to Control Black Dot Disease in Potato
Authors: Kuznetsova Maria A. , Statsyuk Natalia V. , Demidova Valentina N. , Semeniuk Irina N. , Smetanina Tatiana I. , Ukolova Anastasiya Y. , Vyatchinov Alexey A.
Published: June 2024

In recent years, skin blemish diseases of potato (including black dot (BD) caused by Colletotricum coccodes) have begun to be important for global potato marketing, since consumers often reject tubers with an imperfect appearance, which results in financial losses caused by the disposal of unwanted potatoes. Like for many non-fatal plant diseases, BD severity may depend on the immune status of plants influenced by other infectious agents. Using a set of 98 potato cultivars differing in their late blight (LB) resistance, we examined the correlation between the intensity of their infection with LB determined by their LB resistance and the occurrence of the BD disease under field conditions with a high background level of both diseases. Using LB-susceptible (Arizona) and moderately susceptible (Sante) cultivars, we also evaluated the effect of a crop protection against LB on BD development under the same field conditions. A strong negative correlation (r = −0.81, p < 0.05) between the LB resistance and the BD occurrence has been revealed. An experiment using the two cultivars, chemically protected against LB, showed a significant reduction in BD occurrence of 30% (cv. Arizona) and 20% (cv. Sante) compared to the untreated controls; the total yield and marketability of potatoes increased by 103.6 and 62.5% for cv. Arizona and by 65.9 and 43.8% for cv. Sante. The reduction in the LB affection of potato is one of the key factors improving the immune status of potato cultivars in relation to BD infection, so methods of LB protection should be included in a complex approach to BD control.
Assessment of Soil Nutrients in Plant Root Layer of a Saline-Sodic Soil Cropped with Lycium barbarum L. When Drip Irrigated with Saline Groundwater
Authors: Dou Chaoyin , Lv Yuping , Sun Yidi , Chen Xiaoping , Li Yan
Published: June 2024

Drip irrigation is an effective method to utilize waste saline-sodic land with a high water table. For reasonable and sustainable utilization of saline-sodic soil under such conditions, spatiotemporal changes in total nitrogen (TN), total phosphorus (TP), and soil organic matter (SOM) were investigated during the utilization process. The soil was sampled from newly built raised beds before planting (0 y) and beds in three adjacent plots had been planted with Lycium barbarum L. for one (1 y), two (2 y), and three years (3 y), respectively, at the end of the growing season. Soil samples were obtained at four horizontal distances from the drip line (0, 10, 20, and 30 cm) and four vertical soil depths (0–10, 10–20, 20–30, and 30–40 cm). The results showed that the average TN and TP of the soil profile increased with the planting year and were approximately 0.68 and 1.81 g·kg−1 in the soils of 3 y, approximately 84.9 and 42.4% higher than that of 0 y, respectively. SOM decreased in the first growing season and then continuously increased in the following planting years, reaching 8.26 g·kg−1 in the soils of 3 y, which was approximately 38.2% higher than that of 0 y. TN, TP, and SOM contents were high in soils around the drip line and decreased with distance from the drip line. In both horizontal and vertical directions, TN, TP, and SOM varied slightly in soils of 0, 1, and 2 y, while in soils of 3 y, TN and SOM decreased with increased distance in both horizontal and vertical directions and TP decreased obviously only within 10 cm in both directions. This indicated that the contents and distributions of soil nutrients in such saline-sodic soils could be improved with planting year under drip irrigation with local saline groundwater and especially around the drip line as the soil around the dripper was quickly ameliorated.
Effect of Nitrogen and Phosphorus on Soil Enzyme Activities and Organic Carbon Stability in Qinghai–Tibet Plateau
Authors: Zhai Jiaying , Zhou Huakun , Wu Yang , Wang Guoliang , Xue Sha
Published: June 2024

Alpine grassland ecosystems are the most important ecosystem type, exhibiting a high sensitivity to anthropogenic nitrogen (N) and phosphorus (P) inputs into terrestrial ecosystems and significantly affecting the carbon (C) cycle within these ecosystems. However, the effects of N and P additions on soil C stability and the processes of organic C transformation remain unclear. This study measured the soil enzyme activities and oxidizable C fractions over a 9-year period following N and P additions to an alpine meadow in the Qinghai–Tibet Plateau. It included four treatments: control (CK), N addition, P addition, and combined N and P addition (NP), utilizing statistical methods such as analysis of variance (ANOVA), correlation analysis, and redundancy analysis. The findings indicated that NP addition significantly increased the non-labile soil oxidizable C fraction in the topsoil layer. Redundancy and correlation analyses revealed strong associations between the vegetation characteristics, C-cycling enzyme activities, soil-oxidized C fractions, and SOC stability index. These results underscore the role of NP addition in enhancing SOC accumulation and stability in grassland ecosystems, with the soil vegetation properties and C source enzyme activities serving as key regulators of the SOC stability. This study offers valuable insights into predicting the SOC dynamics amid rising N and P availability, thereby elucidating the effect of nutrient addition on soil C-cycling mechanisms.
Histological Variations in Cucumber Grafted Plants and Their Effect on Yield
Authors: Robledo-Torres Valentín , González-Cortés Areli , Luna-García Laura Raquel , Mendoza-Villarreal Rosalinda , Pérez-Rodríguez Miguel Ángel , Camposeco-Montejo Neymar
Published: June 2024

Cucumber is a vegetable crop of economic importance in Mexico. In 2023, fresh or frozen cucumber and pickle international sales reached USD 783M, placing Mexico in second rank among countries with the highest currency inflow in the world. This crop is mostly grown under protected environments with new production technologies, including rootstocks that are tolerant to biotic and abiotic factors that limit the yield and quality of the harvest. Therefore, the goal of this work was to assess three cucurbits as rootstocks (Cucurbita maxima, Cucurbita moschata and Citrullus lanatus), as well as studying the response of cucumber crop to the tested rootstocks. The following treatments were assessed: T1:PSP = cucumber Cv. Paraizo F1 without rootstock; T2:P/MA = cucumber grafted over Cucurbita maxima; T3:P/MO = cucumber grafted over Cucurbita moschata; T4:P/CL = cucumber grafted over Citrullus lanatus Citroides Var. The assessed variables included the number of fruits per plant (NFPP), fruit weight per plant (PFPP), average fruit weight (PPF), plant dry weight (PSP), xylem vessel area (AVX), number of xylem vessels (NVX), vascular bundle area (AHV) and number of vascular bundles (NHV) in roots (R), basal stalk (TB) and apical stalk (AT). Significant differences among treatments were found in the results for variables: NFPP, highlighting that T2:P/MA was superior by 46.15% versus T1:PSP and by 67.69% versus T3:P/MO. Regarding PFPP, T2:P/MA achieved the highest weight (5445 g), as it proved superior by 99.45% than the cucumber without rootstock (T1:PSP). Regarding PSP, T2:P/MA achieved the highest weight (196.65 g), significantly exceeding T1 by 137.5%. Regarding AVXR, T4:P/CL had the highest value (30,063 µm2). Regarding NHVR, watermelon had (T4:P/CL) 39.33% more vascular bundles than the root system of cucumber (T1:PSP). Treatments T2:P/MA and T4:P/CL had the highest values at AVXTB, while for variable NVXTB, treatments T3:P/MO and T2:P/MA were almost equal, surpassing non-grafted cucumber by 175.47%. However, T2:P/MA showed the greatest AHVTB, and T1:PSP was significantly superior to T3:P/MO, by 34.93%, for NHVTB. Furthermore, positive and significant correlations were found among PFPP, AHVR, and AVXTB, as well as among PSP, AVXTB, and AHVTB. Therefore, we may say that having a larger area of vascular vessels in the roots and a larger area of xylem vessels in the basal stalk leads to higher fruit yields per plant. In the same vein, the use of rootstocks is a sustainable alternative to increase fruit yield.
Soil Biopores and Non-Biopores Responses to Different Tillage Treatments in Sugarcane Fields in Guangxi, China
Authors: Wang Song , Gan Lei , Zhang Shuo , Li Jian , Chang Cheng , Zhang Yu , Zhang Hongxia , Wei Benhui
Published: June 2024

Different types of soil macropores respond differently to various tillage practices, owing to disparities in origin, scale, morphology, and function, consequently exerting distinct effects on soil structure. This study aimed to investigate the response mechanisms of three different soil pore types (total macropores, non-biopores, and biopores) to two distinct tillage practices: smash-ridging tillage (T) and no-tillage (NT) in sugarcane fields. The parameters characterizing soil pore treatments in two and three dimensions were obtained using X-ray computed tomography scanning technology. ImageJ and MATLAB software were employed to analyze the data and separate soil macropores into biopores and non-biopores categories. The results showed that non-biopores predominated in two-dimensional cross-sectional areas in NT treatment, whereas biopores were more dominant in T treatment. Biopores in T treatment had a higher proportion of two-dimensional pores compared to NT treatment. A three-dimensional analysis indicated that total macropores had larger mean diameters (MD) and macroporosity, with more continuous tubular pores in T treatment than that in NT treatment. However, NT treatment had more numerous non-biopores with broader spatial distribution and complex morphology. Additionally, biopores in T treatment had larger MD and branching length density (LD). These vertically developed biopores, along with high macropore connectivity and under smash-ridging tillage, could improve soil water and pore conductivity. Therefore, smash-ridging tillage was more beneficial for sugarcane growth compared to no-tillage in Guangxi of China.
Effects of Low-Salinity Stress on Histology and Metabolomics in the Intestine of Fenneropenaeus chinensis
Authors: Tian Caijuan , Wang Qiong , Gao Tian , Sun Huarui , Li Jitao , He Yuying
Published: June 2024

Metabolomics has been used extensively to identify crucial molecules and biochemical effects induced by environmental factors. To understand the effects of acute low-salinity stress on Fenneropenaeus chinensis, intestinal histological examination and untargeted metabonomic analysis of F. chinensis were performed after exposure to a salinity of 15 ppt for 3, 7, and 14 d. The histological examination revealed that acute stress resulted in most epithelial cells rupturing, leading to the dispersion of nuclei in the intestinal lumen after 14 days. Metabolomics analysis identified numerous differentially expressed metabolites (DEMs) at different time points after exposure to low-salinity stress, in which some DEMs were steadily downregulated at the early stage of stress and then gradually upregulated. We further screened 14 overlapping DEMs, in which other DEMs decreased significantly during low-salinity stress, apart from L-palmitoylcarnitine and vitamin A, with enrichments in phenylalanine, tyrosine and tryptophan biosynthesis, fatty acid and retinol metabolism, and ABC transporters. ABC transporters exhibit significant abnormalities and play a vital role in low-salinity stress. This study provides valuable insights into the molecular mechanisms underlying the responses of F. chinensis to acute salinity stress.
Early Embryonic Development in Agriculturally Important Species
Authors: Bazer Fuller W. , Johnson Gregory A.
Published: June 2024

The fertilization of oocytes ovulated by pigs, sheep, cows, and horses is not considered a limiting factor in successful establishment of pregnancy. Pig, sheep, and cow embryos undergo cleavage to the blastocyst stage, hatch from the zona pellucida, and undergo central-type implantation. Hatched blastocysts of pigs, sheep, and cows transition from tubular to long filamentous forms to establish surface area for exchange of nutrients and gases with the uterus. The equine blastocyst, surrounded by external membranes, does not elongate but migrates throughout the uterine lumen before attaching to the uterine luminal epithelium (LE) to begin implantation. Pregnancy recognition signaling in pigs requires the trophectoderm to express interleukin 1 beta, estrogens, prostaglandin E2, and interferon gamma. Sheep and cow conceptus trophectoderm expresses interferon tau that induces interferon regulatory factor 2 that inhibits transcription of estrogen and oxytocin receptors by uterine epithelia. This prevents oxytocin-induced luteolytic pulses of prostaglandin F2-alpha from regressing the corpora lutea, as well as ensuring the secretion of progesterone required for maintenance of pregnancy. The pregnancy recognition signal produced by equine blastocysts is not known. Implantation in these species requires interactions between extracellular matrix (ECM) proteins and integrins as the conceptus undergoes apposition and firm attachment to the uterine LE. This review provides details with respect to early embryonic development and the transition from spherical to filamentous conceptuses in pigs, sheep, and cows, as well as pre-implantation development of equine blastocysts and implantation of the conceptuses.
Echocardiographic Documentation of Dilated Cardiomyopathy Development in Dogs Naturally Infected with Trypanosoma cruzi
Authors: Avalos-Borges Eduardo E. , Acevedo-Arcique Carlos M. , Segura-Correa Jose C. , Jiménez-Coello Matilde , Garg Nisha J. , Ortega-Pacheco Antonio
Published: June 2024

We aimed to characterize the echocardiographic alterations in dogs from an endemic region that were naturally infected with T. cruzi. Dogs (n = 130) seropositive for antibodies against T. cruzi and/or with acute parasitemia were enrolled in the study. Indicators of changes in the structure and systolic and diastolic functions of the left ventricle (LV) and blood flow patterns were evaluated by echocardiography. The frequency and extent of alterations in these indicators were associated with the severity of the disease. Briefly, 15 (11.54%) dogs were diagnosed with dilated cardiomyopathy (DCM), and 115 (88.46%) dogs were diagnosed as being without DCM. Infected dogs with DCM exhibited structural features of LV dysfunction, e.g., a significant (p < 0.05) increase in the LV internal diameter at systole and diastole (LVID-s, LVID-d) and a decline in the LV posterior wall (LVPW-d) thickness at diastole. Despite an increase in stroke volume and cardiac output indicating contraction force, DCM resulted in a decreased ejection fraction, affecting systolic function. Dogs that were diagnosed as DCM-negative but were positive for T. cruzi by PCR exhibited a high frequency of an increase in the thickness of the interventricular septum in systole (IVS-s) and the LV posterior wall in diastole (LVPW-d), a decline in the LV inner diameter (LVID-d, LVID-s), and fractional shortening (FS). The thinning of the LVPW at systole was the most defining feature observed in chronically infected dogs. In summary, this is the first study reporting the echocardiographic changes occurring in dogs naturally infected with T. cruzi and developing DCM. Our data suggest that changes in LVID are a major indicator of risk of cardiac involvement, and the observation of changes in the IVS, LVPW, and FS have predictive value in determining the risk of DCM development in infected dogs.