bluuebunny/crossref_metadata_2025 huggingface dataset:
Maintenance Therapy of Psychosis Spectrum Disorders in a Real-World Setting: Antipsychotics Prescription Patterns and Long-Term Benzodiazepine Use
Authors: Maric Nadja P. , Andric Petrovic Sanja , Russo Manuela , Jerotic Stefan , Ristic Ivan , Savić Bojana , Pemovska Tamara , Milutinovic Milos , Ribic Emina , Markovska-Simoska Silvana , Dzubur Kulenovic Alma , Jovanovic Nikolina
Published: April 2022

Background

Maintenance therapy of patients with primary psychosis spectrum disorders (PSD) in the Western Balkans has received limited interest so far. The present study aimed to investigate long-term prescription patterns among outpatients with PSD.

Methods

Information about prescription of antipsychotics (AP), benzodiazepines (BZD) and other psychotropic medication over a 6-month period was collected from outpatients (n = 134; ICD-10 diagnosis F20-29) recruited by a larger multi-site study, to find mean daily number of psychotropic drugs, AP prescription patterns (including AP daily dose, route of administration, monotherapy vs. polypharmacy) and BZD utilization (long-term add-on BZD therapy). Additionally, sex-differences in the variables were explored.

Results

Clinically stable outpatients (age 41.7 ± 11.0; male 62.7%; duration of untreated illness 12.7 ± 8.7 years; mean number of lifetime hospitalizations 2.6 ± 0.7) were prescribed 2.8 ± 1.1 psychotropic medications daily. The mean 6-month AP dose was 14.2 ± 7.8 mg olanzapine equivalents. Long-acting injectable AP was prescribed to 25.2% of the patients. Long-term AP monotherapy was found in 52.7% patients and most of them were prescribed second generation AP (65.2%). Long-term AP polypharmacy (42.7%) was more common in males (p = 0.015). The most frequent co-prescription patterns were first generation AP plus clozapine. The highest rate of long-term AP co-prescription was found for BZD (in 42.7% cases, average 6-months daily dose of 2.8 ± 2.7 mg lorazepam equivalents) and anticholinergics (33.6%).

Conclusion

Existing appropriately designed interventions aiming to safely switch the inappropriate therapeutic regimens, i.e. very high prevalence of long-term AP polypharmacy and non-rational BZD co-prescription, should be implemented in the region of Western Balkans.
Altered Levels of Plasma Inflammatory Cytokines and White Matter Integrity in Bipolar Disorder Patients With Suicide Attempts
Authors: Jiang Xiaowei , Guo Yingrui , Jia Linna , Zhu Yue , Sun Qikun , Kong Lingtao , Wu Feng , Tang Yanqing
Published: April 2022

Objective

Bipolar disorder (BD) has a higher lifetime rate of suicide attempts (SA) than other psychiatric disorders. Furthermore, BD patients with SA (BD + S) are prone to a worse quality of life. However, the pathophysiology of BD + S is poorly understood. To further reveal the potential mechanisms of BD + S, abnormalities in peripheral plasma inflammatory cytokines and brain white matter (WM) in BD + S, as well as the correlation between them are investigated.

Methods

We tested the levels of TNF-α, IL-1β, and IL-6 in peripheral plasma and collected the diffusion tensor imaging (DTI) data from 14 BD + S, 24 BD patients without SA (BD-S), and 26 healthy controls (HCs). The three groups were matched by age and gender. The levels of TNF-α, IL-1β, and IL-6 were detected by Luminex multifactor detection technology, and the fractional anisotropy (FA) values were employed to depict the alterations of WM. Partial correlation analyses were conducted to detect correlations between levels of TNF-α, IL-1β, and IL-6 and changes of WM, and the relationships between severity of clinical symptoms, including scores of HAMD-17 and YMRS, and cytokine levels or FA values in all groups.

Results

For plasma inflammatory cytokines, there was no significant difference in their levels except for IL-6 among the three groups. Post-hoc analyses revealed that increased IL-6 level was only detected in BD + S (p < 0.05, Bonferroni correction). For DTI, BD + S showed specifically decreased FA in the bilateral middle cerebellar peduncle and the left superior corona radiata compared to BD-S and HCs (p < 0.05, Bonferroni correction). Additionally, both BD + S and BD-S groups revealed decreased FA in the bilateral body and genu of corpus callosum (CC) compared to HCs (p < 0.05, Bonferroni correction). No significant correlation between plasma inflammatory cytokines and WM integrity was found. In the BD + S group, we found negative correlation between the scores of YMRS and FA values of the left middle cerebellar peduncle (r = −0.74, p = 0.035).

Conclusion

The inflammation and impaired WM integrity may provide a scientific basis to understand the potential mechanisms of BD + S.
Profiles of Teacher Professional Identity Among Student Teachers and Its Association With Mental Health
Authors: Zhao Shunying , Dong Yang , Luo Jian
Published: April 2022

In recent years, studies exploring the link between teacher professional identity and mental health are increasing. However, such research using latent profile analysis is still scarce. The aim of this study was to examine the heterogeneity of a sample of 923 student teachers' professional identity and its association with mental health (i.e., psychological wellbeing and anxiety). By using latent profile analysis, four different profiles were identified: (1) low professional identity (LPI, 5.1%), (2) moderate professional identity (MPI, 42.7%), (3) high occupational values (HOV, 12%), and (4) high professional identity (HPI, 40.2%). The results demonstrated that psychological wellbeing and anxiety were significantly different across the four profiles. In addition, the anxiety of HOV group was not significantly with LPI and MPI groups. This study indicated that student teachers in different groups may have different mental health status.
Reviewing the Benefits of Aging Populations: Care Activities Provided by the Older People as a Commons
Authors: Calvo-Sotomayor Iñigo , Atutxa Ekhi
Published: April 2022

This research reviews the commons paradigm as a framework for understanding the positive contribution of older cohorts to society. The commons, thought as instituting praxis that provide social goods based on autonomy and reciprocity, can help in understanding the role of this age group in providing care to the rest of society. This approach overturns the way economic literature approaches the aging phenomenon. What is understood as a “common evil”—or a problem that needs to be solved—can be analyzed as a “common good.” From this perspective, older people (population aged 65 and over) are essential to European society and the economy to carry out informal care activities that enable and facilitate the “productive” activities of working people.
The Black Panther, Masculinity Barriers to Medical Care, and Colorectal Cancer Screening Intention Among Unscreened American Indian/Alaska Native, Black, and White Men
Authors: Brooks Ellen , Islam Jessica Y. , Perdue David G. , Petersen Ethan , Camacho-Rivera Marlene , Kennedy Carson , Rogers Charles R.
Published: April 2022

Objective

To determine if masculinity barriers to medical care and the death from colorectal cancer (CRC) of actor Chadwick Boseman (The Black Panther) influenced CRC early-detection screening intent among unscreened American Indian/Alaska Native (AIAN) and Non-Hispanic-Black (Black) men compared with Non-Hispanic-White (White) men.

Methods

Using a consumer-panel, we surveyed U.S. men aged 18–75 years (N = 895) using the 24-item Masculinity Barriers to Medical Care (MBMC) scale. We calculated the median score to create binary exposures to evaluate associations with CRC screening intent and conducted multivariable logistic regression to evaluate independent associations stratified by race/ethnicity.

Results

Overall, Black respondents were most likely to have a high MBMC score (55%) compared to White (44%) and AIAN (51%) men (p = 0.043). AIAN men were least likely to report CRC screening intent (51.1%) compared with Black (68%) and White men (64%) (p < 0.001). Black men who reported the recent death of Chadwick Boseman increased their awareness of CRC were more likely (78%) to report intention to screen for CRC compared to those who did not (56%) (p < 0.001). Black men who exhibited more masculinity-related barriers to care were more likely to intend to screen for CRC (OR: 1.76, 95% CI: 0.98–3.16) than their counterparts, as were Black men who reported no impact of Boseman's death on their CRC awareness (aOR: 2.96, 95% CI: 1.13–7.67). Conversely, among AIAN men, those who exhibited more masculinity-related barriers to care were less likely to have CRC screening intent (aOR: 0.47, 95% CI: 0.27–0.82) compared with their counterparts.

Conclusions

Masculinity barriers to medical care play a significant role in intention to screen for CRC. While Black men were most likely to state that The Black Panther's death increased their awareness of CRC, it did not appear to modify the role of masculine barriers in CRC screening intention as expected. Further research is warranted to better understand how masculine barriers combined with celebrity-driven health-promotion interventions influence the uptake of early-detection screening for CRC.

Impact

Our study provides formative data to develop behavioral interventions focused on improving CRC screening completion among diverse men.
Sexual Activities and Changes in Condom Use in Group Sex Events Among Female Sex Workers in Melbourne, Australia
Authors: Lew Chen , Fairley Christopher K. , Tran Julien , Phillips Tiffany R. , Aung Ei T. , Maddaford Kate , Chen Marcus Y. , Bradshaw Catriona S. , Chow Eric P. F.
Published: April 2022

Background

There are few studies investigating group sex among female sex workers (FSWs). This study aimed to examine the typical number of group sex participants, sexual activities and condom use in group sex among FSWs attending a sexual health clinic in Melbourne, Australia.

Methods

We conducted a cross-sectional survey between May 1, 2019 and March 13, 2020 among FSWs in Melbourne. Participants were asked whether they had participated in group sex (sex involving more than two participants) within the past 3 months, the size of the most recent group sex event, sexual activities they engaged in and condom use. It is unknown whether this was a paid or unpaid event in our study.

Results

Of the 247 FSWs surveyed, the mean age was 28.9 years and 51.8% were born in Australia. More than a quarter (27.5%, n = 68) had had group sex in the past 3 months with the number of events ranging from 1 to 10 times. The median size of the group was 3 participants with one man and two women (including the FSW) being the most common combination. Kissing was the most common activity in group sex. Of 58 FSWs who had vaginal sex during group sex, 77.3% (51/58) reported their male partners always used condoms but 13.7% (7/51) of these did not change condoms between women.

Conclusion

This study finds that group sex is common among FSWs. Although condoms are always used during group sex, one in six men did not change their condoms between partners, which may increase STI transmission between partners.
Antimicrobial Stewardship in Surgery: A Literature Bibliometric Analysis
Authors: Liu Yang-Xi , Yang Ya , Le Ke-Jia , Zhang Zai-Li , Cui Min , Zhong Han , Gu Zhi-Chun
Published: April 2022

Background

Antimicrobial resistance and the dwindling antibiotic development pipeline have resulted in a looming post-antibiotic era. Research related to antimicrobial stewardship (AMS) has grown rapidly in the past decade, especially in the field of surgery. We conducted a bibliometric analysis of these publications. In addition, we aimed to identify research hotspots and infer future research trends.

Methods

We screened global publications on AMS in the surgical field over ten years (between 2011 and 2020) from the Web of Science core collection database. The keywords “antimicrobial or antibiotic”, “stewardship”, “management”, “management strategies”, “programme”, “surgery” and “surgical” were used to search for related papers. VOS viewer, R software, and other machine learning and visualization tools were used to conduct the bibliometric analysis of the publications.

Results

We identified 674 publications on AMS in surgical fields; “antimicrobial stewardship” (with total link strength of 1,096) was the most frequent keyword, and had strong links to “antimicrobial resistance” and “guidelines”. The top 100 most cited papers had a mean citation count of 47.21 (range: 17–1155) citations, which were cited by survey research studies, clinical trials, and observational studies. The highest-ranking and most cited journal was Clinical Infectious Diseases with eight publications. Jason G. Newland from Washington University wrote seven papers and was cited 1,282 times. The University of Washington published 17 papers and was cited 1,258 times, with the largest number of publications by author and organization. The USA published 198 papers and cooperated with 21 countries, mainly partnering with Italy, the UK, and Canada. Published articles mainly focused on the current clinical situation regarding surgical AMS management, antibiotic prescription, and antibiotic resistance.

Conclusions

Publications on surgical AMS management have increased in recent decades, with the USA being the most prolific. Epidemiological investigations of surgical-related infections, antibiotic prescriptions, and antibiotic resistance are fast-developing research trends. However, further improvements are still needed according to the recommendations gained from the bibliometric analysis.
The UK Antimicrobial Resistance Strategy 2013–18: A Qualitative Study of International and Domestic Policy and Action Related to Livestock and the Food Chain
Authors: Blake Lauren J. , Häsler Barbara , Bennani Houda , Mateus Ana L. P. , Eastmure Elizabeth , Mays Nicholas , Stärk Katharina D. C.
Published: April 2022

Antimicrobial resistance (AMR) is an increasingly important challenge within global health, and growing action is being taken by countries across the world. The UK Government established a national AMR Strategy in 2013, encompassing human and animal health. An evaluation of the implementation of the Strategy was commissioned, to feed into a refresh of the Strategy in 2018. This article addresses the implementation of the parts of the Strategy related to food sourced from animals. It is based on 15 semi-structured interviews with policy officials and professionals from UK and international agencies. Four themes comprise the findings from thematic analysis. (1) The UK's advocacy and leadership at the global level to encourage international action, which was praised. However, the UK's actions were limited in permeating complex international food supply chains. (2) Integrating a One Health approach, adopted in the Strategy, which had successfully facilitated bringing together human and animal health sectors, but still lacked input from environmental agencies and integration of the role of environmental factors in understanding and tackling AMR. (3) Changes in antimicrobial use and attempts to reduce AMR brought about by the Strategy, including the disparity in variations of actions and progress between livestock sectors, the pros and cons of antimicrobial usage reduction targets—felt to be best when adapted for each livestock species sector, the preference for voluntary sector-led approaches to reduction in antimicrobial use, and the need for changes in production systems and animal husbandry. (4) The challenges relating to costs, drivers and incentives identified to reduce antimicrobial use, and how research, data and surveillance systems support continued action. In particular, the need for adequate investment and financial incentives to enable changes in production and husbandry to take a more preventative approach, the importance of working with different actors throughout the food chain in each livestock sector, and the need for adequate and consistent surveillance data to measure antimicrobial use and continued research to understand AMR. The findings have implications for how to implement further changes at both farm and policy level, how to implement harmonized surveillance whilst maintaining sectoral autonomy, the need for a whole food chain approach, and greater integration of research, actions and actors working on environmental factors of AMR.
Assessing the Adaptability of Quinoa and Millet in Two Agroecological Zones of Rwanda
Authors: Habiyaremye Cedric , Ndayiramije Olivier , D'Alpoim Guedes Jade , Murphy Kevin M.
Published: April 2022

Quinoa (Chenopodium quinoa Willd.) and millet species (including Eleusine coracana, Panicum miliaceum, and Setaria italica) are nutritionally valuable seed crops with versatile applications in food production and consumption. Both quinoa and millet have the potential to provide drought-tolerant, nutritious complementary crops to maize that is predominantly cultivated in Rwanda. This study evaluated quinoa and millet genotypes and assessed their agronomic performance in two agroecological zones of Rwanda. Twenty quinoa and fourteen millet cultivars were evaluated for grain yield, emergence, days to heading, flowering, and maturity, and plant height in 2016 and 2017 in Musanze, a highland region (2,254 m above sea level), and Kirehe, in the Eastern lowlands of Rwanda (1,478 m above sea level). Quinoa yield ranged from 189 to 1,855 kg/ha in Musanze and from 140 to 1,259 kg/ha in Kirehe. Millet yield ranged from 16 to 1,536 kg/ha in Musanze and from 21 to 159 kg/ha in Kirehe. Mean cultivar plant height was shorter in Kirehe (μ = 73 and 58 cm for quinoa and millets, respectively), than Musanze (μ = 93 and 76 cm for quinoa and millets, respectively). There was a genotype × environment interaction for maturity in quinoa and millet in both years. Across locations, “Titicaca” and “Earlybird” (Panicum miliaceum) were the earliest maturing quinoa and millet varieties, respectively, both with an average of 91 days to maturity. The results suggest that quinoa and millet have potential as regional crops for inclusion in the traditional dryland cropping rotations in Rwanda, thereby contributing to increased cropping system diversity and food security.
Consumers' Expectations on Transparency of Sustainable Food Chains
Authors: Pozelli Sabio Renata , Spers Eduardo Eugênio
Published: April 2022

The search for food products from sustainable chains has increased in the past years, motivated by consumers' interest in reducing the negative environmental, economic, and health impacts of their food choices. However, it is not yet clear whether transparency expectations of sustainable food chains influence in consumers' perception of this food products. The literature shows that there are gaps in the growth of sustainable product consumption is the transparency of production and the provision of more information to consumers. In this paper, we aimed to better understand what is the role of transparency expectations and how they influence consumers' decision to consume sustainable food products. Based on scales already validated in the literature, a theoretical model with nine hypotheses was proposed. A questionnaire was structured and empirically tested through a survey with 136 consumers of food from alternative networks. Six hypotheses were validated. Three segments of consumers target were identified from an exploratory factor analysis and cluster. Based on the results some marketing actions were suggested for the participants of alternative food networks. Other studies may validate the model proposed here.
Oncological and Functional Outcomes of Transoral Robotic Surgery and Endoscopic Laryngopharyngeal Surgery for Hypopharyngeal Cancer: A Systematic Review
Authors: Lai Katherine W. K. , Lai Ronald , Lorincz Balazs B. , Wang Chen-Chi , Chan Jason Y. K. , Yeung David C. M.
Published: April 2022

Objectives

Hypopharyngeal carcinoma (HPC) is a head and neck carcinoma with poor prognosis. Traditional laryngopharyngectomy offered promising oncological outcomes at the cost of functional outcomes. The recent advent in transoral robotic surgery (TORS), an organ-preserving surgery, has opened up new perspectives in the treatment for HPC. Here, we evaluate minimally invasive organ preservation surgery [TORS and endoscopic laryngopharyngeal surgery (ELPS)] for HPC in terms of feasibility and oncological and functional outcomes.

Methods

This is a systematic review. Six databases [CUHK Full-Text Journals, Embase 1910 to 2021, Ovid Emcare, Ovid MEDLINE (R), CINAHL, PubMed] were searched for articles and primary studies for TORS and ELPS for HPC. Screening was completed using predefined inclusion or exclusion criteria.

Results

A total of 8 studies on TORS and 3 studies on ELPS were eventually chosen after full-text review. For studies on TORS, 61.3% of patients (84 out of 137) still survived at the last follow-up with a mean follow-up time of 23.20 months (range: 12.8–37.21 months). Severe intraoperative and postoperative complications have not been reported. No cases of TORS required a conversion to open surgery. Swallowing function was optimal postoperatively with only 6 patients eventually required a percutaneous endoscopic gastrostomy (PEG) for feeding. Disease-specific survival was taken as the parameter for the measurement of oncological outcomes. A total of 2 studies reported a disease-specific survival of 100% within their follow-up period of 1 and 1.5 years, respectively. Another 2 studies reported a 2-year DSS of 89 and 98%, respectively. A 5-year DSS of 100% in early stage and 74% in late stage were achieved in one study. Another study also reported a 5-year DSS of 91.7%. For studies of ELPS, a 5- and 3-year disease-specific survival of 100% were achieved in 2 studies. Patients who underwent ELPS had good postoperative swallowing function with no PEG placement. There were also no other fatal complications.

Conclusions

Both TORS and ELPS for HPC provide satisfactory long-term oncological and functional outcomes improving postoperative quality of life of patients.
Phenotypic Characterization of a Virulent PRRSV-1 Isolate in a Reproductive Model With and Without Prior Heterologous Modified Live PRRSV-1 Vaccination
Authors: Kreutzmann Heinrich , Stadler Julia , Knecht Christian , Sassu Elena L. , Ruczizka Ursula , Zablotski Yury , Vatzia Eleni , Balka Gyula , Zaruba Marianne , Chen Hann-Wei , Riedel Christiane , Rümenapf Till , Ladinig Andrea
Published: April 2022

Reproductive disorders induced by porcine reproductive and respiratory syndrome virus (PRRSV) cause high economic losses in the pig industry worldwide. In this study, we aimed to phenotypically characterize a virulent PRRSV-1 subtype 1 isolate (AUT15-33) in a reproductive model. Furthermore, the protective effect of a heterologous modified live virus vaccine (ReproCyc® PRRS EU) was evaluated. In addition, PRRSV AUT15-33 was genotypically compared to other well-characterized isolates. Sixteen gilts were equally divided into four groups: a vaccinated and infected group (V–I), a vaccinated and non-infected group (V–NI), a non-vaccinated and infected group (NV–I), and a non-vaccinated and non-infected (NV–NI) group. After PRRSV infection on gestation day 84, all gilts were clinically examined on a daily basis, and blood samples were taken at five timepoints. Necropsy was performed 3 weeks after infection. The fetal preservation status was assessed, and PRRSV RNA concentrations were measured in the blood and tissue samples from all gilts and fetuses. After infection, all four gilts in the NV–I group were viremic throughout 17 days post-infection (dpi), whereas two gilts in the V–I group were viremic at only one timepoint at 6 dpi. The viral load was significantly higher in gilt serum, tracheobronchial lymph nodes, uterine lymph nodes, maternal endometrium, and fetal placenta of NV–I gilts compared to the V–I ones (p< 0.05). Moreover, the preservation status of the fetuses derived from NV–I gilts was significantly impaired (55.9% of viable fetuses) compared to the other groups (p< 0.001). Upon comparison with other known isolates, the phylogenetic analyses revealed the closest relation to a well-characterized PRRSV-1 subtype 1 field isolate from Belgium. In conclusion, the high virulence of AUT15-33 was phenotypically confirmed in an experimental reproductive model. The vaccination of the gilts showed promising results in reducing viremia, fetal damage, and transplacental transmission of the PRRSV-1 strain characterized in this study.
Pathogen Screening for Possible Causes of Meningitis/Encephalitis in Wild Carnivores From Saxony-Anhalt
Authors: Höche Jennifer , House Robert Valerio , Heinrich Anja , Schliephake Annette , Albrecht Kerstin , Pfeffer Martin , Ellenberger Christin
Published: April 2022

Inflammation in meninges and/or brain is regularly noticed in red foxes and other wild carnivores during rabies control programs. Despite negative rabies virus (RABV) results, the etiologies of these cases remain unknown. Thus, the aim of this study was to provide an overview of the occurrence of pathogens that may cause diseases in the brains of wild carnivores and pose a risk to humans and other animals. In addition to RABV and canine distemper virus (CDV), a variety of pathogens, including members of Flaviviridae, Bornaviridae, Herpesviridae, Circoviridae, as well as bacteria and parasites can also cause brain lesions. In 2016 and 2017, brain samples of 1,124 wild carnivores were examined by direct fluorescent antibody test for RABV as well as (reverse-transcriptase) quantitative polymerase chain reaction (PCR) for the presence of CDV as part of a monitoring program in Saxony-Anhalt, Germany. Here, we applied similar methods to specifically detect suid herpesvirus 1 (SuHV-1), West Nile virus (WNV), Borna disease virus 1 (BoDV-1), canid alphaherpesvirus 1 (CaHV-1), canine parvovirus type 2 (CPV-2), fox circovirus (FoxCV), and Neospora caninum (N. caninum). Further, bacteriogical examination for the existence of Listeria monocytogenes (L. monocytogenes) and immunohistochemistry of selected cases to detect Toxoplasma gondii (T. gondii) antigen were performed. Of all pathogens studied, CDV was found most frequently (31.05%), followed by FoxCV (6.80%), CPV-2 (6.41%), T. gondii (4/15; 26.67%), nematode larvae (1.51%), L. monocytogenes (0.3%), and various other bacterial pathogens (1.42%). In 68 of these cases (6.05%), multiple pathogen combinations were present simultaneously. However, RABV, WNV, BoDV-1, SuHV-1, CaHV-1, and N. caninum were not detected. The majority of the histopathological changes in 440 animals were inflammation (320/440; 72.73%), predominantly non-suppurative in character (280/320; 87.50%), and in many cases in combination with gliosis, satellitosis, neuronophagia, neuronal necrosis, and/or vacuolization/demyelination, or in single cases with malacia. Thus, it could be shown that wild carnivores in Saxony-Anhalt are carriers mainly for CDV and sometimes also for other, partly zoonotic pathogens. Therefore, the existing monitoring program should be expanded to assess the spill-over risk from wild carnivores to humans and other animals and to demonstrate the role of wild carnivores in the epidemiology of these zoonotic pathogens.
Dietary Supplementation With Yucca Alleviates Heat Stress in Growing Broilers Exposed to High Ambient Temperature
Authors: Luo Jing Jing , Chen Wei , Qu Hao , Liu Yuan Qing , Luo Cheng Long , Ji Jian , Shu Ding Ming , Wang Jie
Published: April 2022

Yucca contains high a content of saponin that has a glucocorticord-like effect in animals, e.g., anti-inflammation and anti-microbiota. The objective of the present study was to test the hypothesis that dietary supplementation of yucca powder may alleviate heat stress and improve growth performance of growing broilers subjected to cycling high ambient temperature. A total of 240 male broiler chicks (yellow feathered chicken) aged 28 days, with body weight (BW) of 792 ± 43.7 g, were randomly allocated to one of four treatments (6 replicates per treatment): control (normal temperature, 24 ± 2°C, 24 h), fed diets supplemented with 100 mg/kg yucca under normal temperature (Y), high ambient temperature exposure (HT, 34 ± 2°C, 11 h), fed diets supplemented with 100 mg/kg yucca (HT+Y) under high ambient temperature. After 7 days of adaption, the experiment was conducted for 4 weeks (aged 28–56 days). HT significantly reduced feed intake, BW, and average daily gain (ADG) of broiler, but yucca improved the feed intake under HT condition. Yucca supplementation reduced (P < 0.05) the HT-induced increase in temperature of rectum and leg skin. Supplementation of yucca increased the hypothalamic mRNA expression of TRPV2, TRPV4, and TRPM8 (P < 0.05). Yucca reduced (P < 0.05) the plasma lipid oxidation product malondialdehyde (MDA), but did not affect the activities of antioxidant enzyme superoxide oxidase (SOD) and glutathione peroxidase (Gpx). Yucca did not affect the plasma neuro peptide Y (NPY), which was reduced by HT, yucca reduced circulation cholecystokinin (CCK) and hypothalamic mRNA expression of CCK. Supplementation of yucca increased the mRNA expression of both heat and cool sensing receptors. The results of the present study indicate that yucca could improve antioxidant status and attenuate the heat stress response by regulating hypothalamic temperature-sensing genes in growing chickens. Besides, yucca supplementation improved feed intake probably through modulating CCK in growing broilers under high ambient temperature.
Hematological and Histopathological Effects of Subacute Aconitine Poisoning in Mouse
Authors: Lu Hao , Mei Li , Guo Ziyu , Wu Kexin , Zhang Yunhao , Tang Shiyu , Zhu Yiru , Zhao Baoyu
Published: April 2022

Aconitine is the principal toxic ingredient of Aconitum, which can cause systemic poisoning involving multiple organs and systems after animal ingestion. The purpose of this study was to investigate the effects of aconitine on hematological indices and histological changes in mice. One hundred twenty mice were divided into a control group (normal saline), low-dose group (0.14 μmol/L), middle-dose group (0.28 μmol/L) and high-dose group (0.56 μmol/L), which were continuously lavaged for 30 days. The blood of 10 mice were collected randomly and analyzed by group at the 10th, 20th, and 30th days, and some tissues were collected and stained with hematoxylin-eosin to observe histological changes at the 30th day. Compared with the control group, the organ coefficient (%) of liver, spleen, lungs, and brain of the high-dose group were significantly increased (p < 0.05 or p < 0.01). WBC and Gran initially decreased and then increased in each poisoning group, with significant differences in the high-dose group (p < 0.05 or p < 0.01). RBC, HGB, HCT, and PLT decreased continuously in all groups except the low-dose group at the 20th and 30th days (p < 0.05 or p < 0.01). Moreover, BUN, ALT and AST increased in each poisoning group, in comparison with the control group, with significant differences except for the low-dose group (p < 0.05 or p < 0.01). CRE initially increased and then decreased, the TP and ALB decreased, with significant differences observed in the high-dose and middle-dose groups (p < 0.05). All the mice in the poison-treated groups showed varying degrees of histopathological changes such as degeneration and necrosis of tissues, especially heart and cerebellum. Our data suggest that different doses of aconitine have remarkable effects on hematological and histopathological changes in mice, in a significant time and dose-effect relationship.
Situating Health Within the 2030 Agenda—A Practical Application of the Sustainable Development Goals Synergies Approach
Authors: Helldén Daniel , Weitz Nina , Nilsson Måns , Alfvén Tobias
Published: April 2022

Background: The Sustainable Development Goals (SDGs) are integrated, indivisible and interdependent and interact and affect each other directly and indirectly. However, the 2030 Agenda does not attempt to identify or characterise these interactions.

Evidence: The SDG Synergies approach was developed to enable the investigation of the strength and nature of interactions between SDGs based on the perception of a multistakeholder group. Two examples are given to concretely demonstrate how this approach overcomes present challenges and can be applied to situate health within the 2030 Agenda.

Policy Options and Recommendations: There are clear benefits to situate desired health policy outcomes within the 2030 Agenda, and the SDG Synergies approach can be used as lever for including health aspects in traditional non-health sectors. Although focusing on specific health policies cannot be substituted with multisectoral policies alone, utilizing tools and methods such as the SDG Synergies approach can help policy makers put health at the centre of the SDGs.

Conclusion: SDG Synergies is an impactful approach for policy makers to gain a systemic understanding of how broader sustainable development shape the health and well-being of people and vice versa.
A Soft Self-Stable Actuator and Its Energy-Efficient Grasping
Authors: Luo Ji , Jiang Pei , Li Xiaobin , Bai Long , Liu Fuqiang , Chen Rui
Published: April 2022

Due to high compliance and adaptiveness, soft robots show more advantages than traditional rigid robots in grasping irregularly shaped or fragile objects. Moreover, soft robots attract increasing attention as more and more robots are adopted in unstructured or human–robot interaction environments. However, during the grasping process, most soft robots need constant input stimuli to maintain the grasping configurations, which directly induces low energy efficiency. This article demonstrates the self-stable property of the dual-chamber structure, which can maintain bending deformation without input stimuli based on the differential pressure of the two chambers. Based on the self-stable property, a soft self-stable actuator (SSSA) driven by the tendon is proposed, and it can maintain the bending deformation without energy consumption, which can improve the energy efficiency of the actuator significantly. The self-stability of the actuator during the restoring period is analyzed, and the energy consumption model of the actuator is developed. Finally, the experiments are conducted to verify the validity of the models. The results show the actuator can achieve self-stability without any energy input, and the energy consumption can be reduced by about 45%. Hence, the proposed actuator can be adopted in applications where energy efficiency is sensitive.
Ethnic Identity Development, Post-Traumatic Stress Symptoms, and Relationships with Primary Caregivers: A Two-Wave Longitudinal Study among Yi Ethnic Minority Youths in Rural China School Settings
Authors: Lai Angel Hor-Yan , Yao Hong , Chen Mengtong , Lau Wendy Suet-Yee
Published: April 2022

Background. This study examined the effects of post-traumatic stress symptoms and child–primary caregiver relationships on the ethnic identity of Yi ethnic minority adolescents (n = 550; aged 13–18) in rural China over one school year. The moderating effect of caregivers’ relationships over time and ethnic identity, and post-traumatic stress and ethnic identity, were investigated. Methods. Participants completed two waves of the survey. Longitudinal multilevel modeling was used. Results. (1) Ethnic identity exploration increased over time, while ethnic identity commitment remained stable. (2) Post-traumatic stress symptoms led to high levels of ethnic identity exploration and commitment. (3) Participants with distinct caregivers’ relationship patterns, i.e., secured, optimal, deprived, and disengaged, differed in their ethnic identity exploration scores, but not in ethnic identity commitment. (4) While the secured group scored highest in their ethnic identity development over time, youths who felt disengaged with their caregivers showed the fastest rate of positive change in their ethnic identity exploration. (5) Having a secured caregivers’ relationship weakened the effect of post-traumatic stress on ethnic identity exploration. Conclusion. Practical implications for the meaning of ethnic identity exploration, the promoting effect of caregivers’ relationships, and the negative impact of post-traumatic stress among ethnic minority youths were discussed. Future research directions were also proposed.
Irrigation Water Management Tools and Alternative Irrigation Sources Trends and Perceptions by Farmers from the Delta Regions of the Lower Mississippi River Basin in South Central USA
Authors: Quintana-Ashwell Nicolas , Gholson Drew , Kaur Gurpreet , Singh Gurbir , Massey Joseph , Krutz L. Jason , Henry Christopher G. , Cooke Trey , Reba Michele , Locke Martin A.
Published: April 2022

This article describes the opinions and perceptions of farmers on water management tools that conserve groundwater and on alternative sources of water for irrigation. The analysis is based on a survey of producers (N=466) across the Lower Mississippi River Basin (LMRB) areas of Arkansas, Louisiana, Mississippi, and Missouri. Summary statistics of practice usage across the region and for each state are presented. A Poisson count model is applied to the data to identify factors that influence the number of groundwater-conserving practices employed. The number of irrigated acres, years of farming, annual income level, perception of groundwater problems, and participation in conservation programs have statistically significant association with the number of practices employed. Years of farming experience is the only factor negatively associated with the number of practices employed, while participation in conservation programs has the largest magnitude effect on that number. These results provide evidence that sponsored conservation programs increase the number of conservation practices adopted by farmers. This insight is useful for producer collectives, policy makers, and program managers to design and target of conservation programs across the LMRB.
Combined Application of Organic and Inorganic Amendments Improved the Yield and Nutritional Quality of Forage Sorghum
Authors: Sher Ahmad , Adnan Muhammad , Sattar Abdul , Ul-Allah Sami , Ijaz Muhammad , Hassan Muhammad Umair , Manaf Abdul , Qayyum Abdul , Elesawy Basem H. , Ismail Khadiga Ahmed , Gharib Amal F. , El Askary Ahmad
Published: April 2022

The use of organic amendments is seen to be a promising method for enhancing crop productivity and soil health. Therefore, this study was performed for two consecutive years (2019 and 2020) to determine the effects of organic biochar (BC), sugar industry press mud (MUD), and poultry manure (PM) combined with inorganic amendments on the yield and nutritional quality of forage sorghum at the College of Agriculture, Bahauddin Zakariya University, Bahadur sub-campus, Layyah, Pakistan. The treatments were comprised of the following: control (no inorganic or organic amendments added); recommended dose of NPK (59:72:30 kg ha−1); half dose of NPK (29.5:36:15 kg ha−1); recommended dose of poultry manure (PM) at 5 t ha−1; recommended dose of press mud (MUD) at 40 t ha−1; recommended dose of biochar (BC) at 11 t ha−1; BC + half NPK; MUD + half NPK; PM + half NPK; PM + BC + half NPK; PM + MUD + half NPK; BC + MUD + half NPK; PM + BC + MUD + half NPK. The treatments were carried out in a triplicate randomized complete block design. Results revealed that combined application of PM + BC + MUD + 1/2 NPK significantly enhanced the plant height (201 cm), number of leaves (17), stem diameter (18 mm), stem dry weight (201.7 g), leaf dry weight (30.4 g), leaf area (184.3 cm2), green forage yield (31.8 Mg ha−1), and dry biomass yield (12.7 Mg ha−1) compared with the control treatment. Forage quality traits, including crude protein (CP), brix percentage, acid detergent fiber (ADF), and acid detergent lignin (ADL), showed maximum value with the combined application of PM + BC + MUD + 1/2 NPK. ADF and ADL are linked with lower digestibility; therefore, it was concluded that the combined application of PM + BC + MUD + 1/2 NPK can improve the productivity, dry biomass yield, and CP of sorghum, but reduces the digestibility under semi-arid conditions, such as those in Central Pakistan.
Composition and Fatty Acid Profile of Bone Marrow in Farmed Fallow Deer (Dama dama) Depending on Diet
Authors: Steiner-Bogdaszewska Żaneta , Tajchman Katarzyna , Domaradzki Piotr , Florek Mariusz
Published: April 2022

There are few studies on the composition of fatty acids and how they change the bone marrow fat of young animals depending on nutrition. Therefore, the proximate and fatty acid composition of metatarsal bone marrow from fawns of farm fallow deer after a summer of grazing and the winter feeding was compared. Due to the size and nature of the data, parametric or nonparametric tests were used. Fatty acid composition was determined by gas chromatographic analysis. After the winter feeding, bone marrow contained more fat (83.11% vs. 75.09%, p < 0.05) and less fat free dry matter (5.61% vs. 13.76%, p < 0.05) compared to the pasture period. Moreover, there was a significantly higher amount of saturated fatty acids (23.34% vs. 21.60%, p < 0.001), more trans fatty acids (2.99% vs. 2.34%, p < 0.005), and conjugated linoleic acid isomers (1.04% vs. 0.83%, p < 0.01), compared to post winter feeding, which in turn contained significantly more total cis-monounsaturated fatty acids (54.65% vs. 58.90%, p < 0.001). The percentage of polyunsaturated fatty acids (including n-3 and n-6) was not affected by feeding season. In conclusion, it was shown that young male farm fallow deer were better nourished after the winter period, during which they were kept in properly prepared rooms and fed fodder prepared by people.
Latitudinal Variation in the Pattern of Temperature-Dependent Sex Determination in the Japanese Gecko, Gekko japonicus
Authors: Li Shuran , Xu Zhiwang , Luo Laigao , Ping Jun , Zhou Huabin , Xie Lei , Zhang Yongpu
Published: April 2022

Identifying latitudinal variation in the pattern of temperature-dependent sex determination (TSD) may provide insight into the evolution of sex determining system in vertebrates, but such studies remain limited. Here, we quantified TSD patterns of three geographically separated populations of the Japanese gecko (Gekko japonicus) along the latitudinal cline of China. We incubated gecko eggs from the three populations at constant temperatures of 24, 26, 28, 30, and 32 °C to quantify the TSD pattern. Our study demonstrated that G. japonicus exhibited a FMF pattern of TSD, with the low and high incubation temperatures yielding significantly female-biased hatchlings, and the medium temperatures producing male-biased hatchlings. More interestingly, we found latitudinal variations in the TSD pattern in terms of pivotal temperatures (Tpivs), transitional range of temperatures (TRT), and the sex ratios at the medium temperatures. The Tpivs for the low-latitude population were lower than those for the two high-latitude populations. The low-latitude population has a narrower FM TRT, but a wider MF TRT. The sex ratio is almost 50:50 for the low-latitude population when eggs were incubated from 26 to 30 °C. Conversely, the sex ratio is male-biased for the two high-latitude populations at 28 or 30 °C. Therefore, G. japonicus may provide an interesting system to explore the evolution of TSD in reptiles given the diversity of TSD patterns among populations.
Ocular Microbiome in a Group of Clinically Healthy Horses
Authors: Santibáñez Rodrigo , Lara Felipe , Barros Teresa M. , Mardones Elizabeth , Cuadra Françoise , Thomson Pamela
Published: April 2022

The ocular microbiome in horses is poorly described compared to other species, and most of the information available in the literature is based on traditional techniques, which has limited the depth of the knowledge on the subject. The objective of this study was to characterize and predict the metabolic pathways of the ocular microbiome of a group of healthy horses. Conjunctival swabs were obtained from both eyes of 14 horses, and DNA extraction was performed from the swabs, followed by next generation sequencing and bioinformatics analyses employing DADA2 and PICRUSt2. A total of 17 phyla were identified, of which Pseudomonadota (Proteobacteria) was the most abundant (59.88%), followed by Actinomycetota (Actinobacteria) (22.44%) and Bacteroidota (Bacteroidetes) (16.39%), totaling an average of 98.72% of the communities. Similarly, of the 278 genera identified, Massilia, Pedobacter, Pseudomonas, Sphingomonas, Suttonella and Verticia were present in more than 5% of the samples analyzed. Both Actinobacteria and Bacteroides showed great heterogeneity within the samples. The most abundant inferred metabolic functions were related to vital functions for bacteria such as aerobic respiration, amino acid, and lipid biosynthesis.
Citrullus colocynthis Seed Ameliorates Layer Performance and Immune Response under Acute Oxidative Stress Induced by Paraquat Injection
Authors: Abbas Ahmed O. , Alaqil Abdulaziz A. , Kamel Nancy N. , Moustafa Eman S.
Published: April 2022

Oxidative stress is a detrimental physiological state that threatens birds’ productivity and general health. Colocynth is an herbal plant known for its bioactive properties, and it is mainly known for its antioxidant effects. This study’s purpose was to investigate how effective colocynth seed is at lowering the detrimental impact of acute oxidative stress caused by paraquat (PQ) injection in laying hens. A total of 360 Hy-Line Brown chickens, aged 39 weeks, were gathered and divided into four equal groups (10 hens × 9 replicates) in a 2 × 2 factorial design. The experimental groups were given either a basal diet or the basal diet supplemented with colocynth seed (1% of diet). Starting from week 40 of age and for 7 successive days, the experimental groups were either injected daily with paraquat (5 mg/kg body weight) or with saline (0.5 mL, 0.9% NaCl). Egg production performance with selected stress biomarkers and immunological response parameters were investigated at the end of week 40 of age. Our data revealed a significant reduction in egg production with an increase in blood stress biomarkers (i.e., HSP-70, corticosterone, and H/L ratio) in PQ-injected groups compared with non-stressed groups. Furthermore, an unbalanced redox state was detected in acute oxidative stress groups, with a significant rise in lipid peroxidation level, a reduction in total antioxidant capacity (TAC), and a drop in superoxide dismutase (SOD) and catalase enzyme activity. Supplementing PQ-injected hens with colocynth seed reduced the deleterious effects of acute oxidative stress. There was a significant drop in stress biomarkers with a significant rise in antioxidant enzyme activity and TAC observed in the PQ-injected group provided with colocynth seed supplementation. Remarkably, supplementation of colocynth in the non-stressed group resulted in a significant 27% increase in TAC concentration and 17% higher SOD activity when compared with the non-stressed control group. Colocynth supplementation in the PQ-injected group elevated the total white blood cell count by 25% and improved the B-lymphocyte proliferation index (a 1.3-fold increase) compared with the PQ-injected group that did not receive supplementation. Moreover, the non-stressed colocynth-supplemented group had significantly higher cell-mediated and humoral immune responses than the non-stressed control group. This study demonstrated that colocynth seed supplementation in birds exposed to acute oxidative stress may effectively alleviate its negative impacts on production performance, immunological responses, and redox status. We also inferred that, under normal conditions, colocynth seed can be added to laying hens’ diets to stimulate production and ameliorate immune responses.