bluuebunny/crossref_metadata_2025 huggingface dataset:
Sulforaphane Exposure Prevents Cadmium-Induced Toxicity and Mitochondrial Dysfunction in the Nematode Caenorhabditis elegans by Regulating the Insulin/Insulin-like Growth Factor Signaling (IIS) Pathway
Authors: Hernández-Cruz Estefani Yaquelin , Aparicio-Trejo Omar Emiliano , Eugenio-Pérez Dianelena , Juárez-Peredo Elí , Zurita-León Mariana , Valdés Víctor Julián , Pedraza-Chaverri José
Published: May 2024

Cadmium (Cd) is a heavy metal that is highly toxic to humans and animals. Its adverse effects have been widely associated with mitochondrial alterations. However, there are not many treatments that target mitochondria. This study aimed to evaluate the impact of sulforaphane (SFN) pre-exposure against cadmium chloride (CdCl2)-induced toxicity and mitochondrial alterations in the nematode Caenorhabditis elegans (C. elegans), by exploring the role of the insulin/insulin-like growth factor signaling pathway (IIS). The results revealed that prior exposure to SFN protected against CdCl2-induced mortality and increased lifespan, body length, and mobility while reducing lipofuscin levels. Furthermore, SFN prevented mitochondrial alterations by increasing mitochondrial membrane potential (Δψm) and restoring mitochondrial oxygen consumption rate, thereby decreasing mitochondrial reactive oxygen species (ROS) production. The improvement in mitochondrial function was associated with increased mitochondrial mass and the involvement of the daf-16 and skn-1c genes of the IIS signaling pathway. In conclusion, exposure to SFN before exposure to CdCl2 mitigates toxic effects and mitochondrial alterations, possibly by increasing mitochondrial mass, which may be related to the regulation of the IIS pathway. These discoveries open new possibilities for developing therapies to reduce the damage caused by Cd toxicity and oxidative stress in biological systems, highlighting antioxidants with mitochondrial action as promising tools.
Superoxide Dismutase Mimetic Avasopasem Manganese Enhances Radiation Therapy Effectiveness in Soft Tissue Sarcomas and Accelerates Wound Healing
Authors: Zaher Amira , Mapuskar Kranti A. , Petronek Michael S. , Tanas Munir R. , Isaacson Alexandra L. , Dodd Rebecca D. , Milhem Mohammed , Furqan Muhammad , Spitz Douglas R. , Miller Benjamin J. , Beardsley Robert A. , Allen Bryan G.
Published: May 2024

Soft tissue sarcomas (STSs) are mesenchymal malignant lesions that develop in soft tissues. Despite current treatments, including radiation therapy (RT) and surgery, STSs can be associated with poor patient outcomes and metastatic recurrences. Neoadjuvant radiation therapy (nRT), while effective, is often accompanied by severe postoperative wound healing complications due to damage to the surrounding normal tissues. Thus, there is a need to develop therapeutic approaches to reduce nRT toxicities. Avasopasem manganese (AVA) is a selective superoxide dismutase mimetic that protects against IR-induced oral mucositis and lung fibrosis. We tested the efficacy of AVA in enhancing RT in STSs and in promoting wound healing. Using colony formation assays and alkaline comet assays, we report that AVA selectively enhanced the STS (liposarcoma, fibrosarcoma, leiomyosarcoma, and MPNST) cellular response to radiation compared to normal dermal fibroblasts (NDFs). AVA is believed to selectively enhance radiation therapy by targeting differential hydrogen peroxide clearance in tumor cells compared to non-malignant cells. STS cells demonstrated increased catalase protein levels and activity compared to normal fibroblasts. Additionally, NDFs showed significantly higher levels of GPx1 activity compared to STSs. The depletion of glutathione using buthionine sulfoximine (BSO) sensitized the NDF cells to AVA, suggesting that GPx1 may, in part, facilitate the selective toxicity of AVA. Finally, AVA significantly accelerated wound closure in a murine model of wound healing post RT. Our data suggest that AVA may be a promising combination strategy for nRT therapy in STSs.
Empirical Study of Fully Homomorphic Encryption Using Microsoft SEAL
Authors: Valera-Rodriguez Francisco-Jose , Manzanares-Lopez Pilar , Cano Maria-Dolores
Published: May 2024

In the context of the increasing integration of Internet of Things technologies and the growing importance of data lakes, the need for robust cybersecurity measures to protect privacy without compromising data utility becomes key. Aiming to address the privacy–security challenge in such digital ecosystems, this study explores the application of Fully Homomorphic Encryption (FHE) using the Microsoft SEAL library. FHE allows for operations on encrypted data, offering a promising opportunity for maintaining data confidentiality during processing. Our research employs systematic experimental tests on datasets to evaluate the performance of homomorphic encryption in terms of CPU usage and execution time, executed across traditional PC configurations and a NVIDIA Jetson Nano device to assess the scalability and practicality of FHE in edge computing. The results reveal a performance disparity between computing environments, with the PC showing stable performance and the Jetson Nano revealing the limitations of edge devices in handling encryption tasks due to computational and memory constraints.
GreenTray®, a TIS Bioreactor for Plant Micropropagation and Abiotic or Biotic Stress Bioassays
Authors: Dolcet-Sanjuan Ramon , Casanovas Maria , Franquesa Sandra , Alsina Eva , Carrasco-Cuello Francisca , Torres Estanis , Rufat Josep , Solsona Cristina , Teixido Neus
Published: May 2024

The temporary immersion system bioreactor named GreenTray® presents advantages over the existing ones. Firstly, there is no need to use forceps to move the shoots or plantlets in or out of the recipient, nor is the use of a scalpel necessary to divide them. Secondly, the basis of the shoot can remain in the GreenTray® and can sprout again over several cycles of growth. These two characteristics reduce the costs in the process of micropropagation. In addition, shoot or plantlet development is enhanced by the aeration of the recipient, thus enriching the recipient’s atmospheric CO2. This article describes and provides images and values of interest in (1) the micropropagation process of some commercial Prunus or Pyrus rootstocks; (2) the plantlet growth under autotrophic conditions; and (3) the inoculation of plants with pathogens that reproduce in vitro the symptoms observed in vivo, using the GreenTray® bioreactor.
Enhancing the Extraction of Phenolic Antioxidants from Amazonian Assai (Euterpe oleracea Martius) Fruit Waste through Response Surface Methodology Optimization
Authors: Cunha-Silva Yasmin , Dias David , Pereira Luiz Felipe Ravazi , Pereira Carlos Victor Lamarão , Lima Emerson Silva , Yamaguchi Klenicy Kazumy de Lima , Veiga-Junior Valdir Florêncio da
Published: May 2024

Assai (Euterpe oleracea Martius) is a superfruit widely consumed in several countries, mainly produced in the Amazon region. The significant growth of the market for this fruit has raised environmental concerns regarding the disposal of production waste, especially the seeds that are not utilized and represent approximately 80% of the fruit. In the present study, strategies were developed for transforming these seed wastes into new products, using green solvents for extraction under conditions conducive to technology transfer and with feasible quality control through simple bench techniques, which represents an ideal approach for establishing a truly sustainable process. A significant interaction between solvent and extraction method was observed, impacting both yields and total phenols. Phenolic compounds are substances known for their health benefits, functioning as antioxidants and consequently aiding in disease prevention. The phenolic content observed in the extractions increased from 22.68% to 44.74% under optimal conditions during extraction via hot maceration (50 °C) in 100% ethanol for 2 h, which also enhanced yield and increased antioxidant capacity. The extracts displayed remarkable free radical scavenging activities (IC50 = 6.54 μg/mL in ABTS and IC50 = 14.71 μg/mL in DPPH), approaching the Trolox and quercetin standards, respectively. The optimized method paved the way for the industrial-scale utilization of the residues of this valuable Amazonian fruit.
Production of Anthocyanin-Rich Red Rose Petal Extract by Enzymatic Maceration
Authors: Ribeiro Bernardo Dias , Ferreira Rachel de Moraes , Coelho Liliana Areia Bastos , Barreto Daniel Weingart
Published: May 2024

The use of enzymes to hydrolyze the plant cell matrix is a method known for extracting bioactive substances. The current work used this strategy to produce a rose petal extract rich in anthocyanins that is stable in the presence of marine polysaccharides and has a high antioxidant activity. The process evaluation was carried out sequentially, initially comparing water, ethanol, and their mixtures to anthocyanins extracted in the presence or absence of enzymes. Then, a multi-objective desirability function optimized experimental conditions such as solvent and enzyme concentrations. This study is the first report describing the use of a statistical tool, the central composite rotatable design (CCRD), to optimize anthocyanin extraction from rose petals. This method obtained a maximum extraction of 9.99 mg/g of phenols. The stability of the rose petal extract when using marine polysaccharides retained 60% of the anthocyanins over 28 days without deterioration when protected from sunlight but was practically degraded upon exposure to sunlight. The rose petal extract demonstrated a very high antioxidant capacity of 3.19 μg/mL, close to the literature data for citrus compounds, known to be high in antioxidant compounds for cosmetic food purposes.
Therapy-Related Myeloid Neoplasm: Biology and Mechanistic Aspects of Malignant Progression
Authors: Travaglini Serena , Marinoni Massimiliano , Visconte Valeria , Guarnera Luca
Published: May 2024

Therapy-related myeloid neoplasms (t-MN) arise after a documented history of chemo/radiotherapy as treatment for an unrelated condition and account for 10–20% of myelodysplastic syndromes and acute myeloid leukemia. T-MN are characterized by a specific genetic signature, aggressive features and dismal prognosis. The nomenclature and the subsets of these conditions have changed frequently over time, and despite the fact that, in the last classification, they lost their autonomous entity status and became disease qualifiers, the recognition of this feature remains of major importance. Furthermore, in recent years, extensive studies focusing on clonal hematopoiesis and germline variants shed light on the mechanisms of positive pressure underpinning the rise of driver gene mutations in t-MN. In this manuscript, we aim to review the evolution of defining criteria and characteristics of t-MN from a clinical and biological perspective, the advances in mechanistic aspects of malignant progression and the challenges in prevention and management.
Vascular Endothelial Growth Factor (VEGF) and Its Role in the Cardiovascular System
Authors: Florek Kamila , Mendyka Dominik , Gomułka Krzysztof
Published: May 2024

Cardiovascular diseases remain the leading cause of death worldwide, with ischemic heart disease (IHD) as the most common. Ischemia-induced angiogenesis is a process in which vascular endothelial growth factor (VEGF) plays a crucial role. To conduct research in the field of VEGF’s association in cardiovascular diseases, it is vital to understand its role in the physiological and pathological processes in the heart. VEGF-based therapies have demonstrated a promising role in preclinical studies. However, their potential in human therapies is currently under discussion. Furthermore, VEGF is considered a potential biomarker for collateral circulation assessment and heart failure (HF) mortality. Additionally, as VEGF is involved in angiogenesis, there is a need to elucidate the impact of VEGF-targeted therapies in terms of cardiovascular side effects.
Influence of Biofilm Maturity on the Antibacterial Efficacy of Cold Atmospheric Plasma in Oral Microcosm Biofilms
Authors: Kim Hee-Eun
Published: May 2024

As biofilms mature, biomass and extracellular polysaccharide (EPS) content increases, enhancing pathogenicity. Therefore, this study aimed to evaluate the antibacterial efficacy of cold atmospheric plasma (CAP) against oral microcosm biofilms and the influence of biofilm maturity on treatment. Oral microcosm biofilms were cultured on hydroxyapatite disks for 2 and 6 days. Based on the treatment and biofilm maturity, these were subsequently allocated into six groups (N = 19 each): Groups 1 and 2 were incubated with distilled water for 1 min; Groups 3 and 4 were treated with CAP for 2 min, and Groups 5 and 6 were treated with 0.12% chlorhexidine gluconate for 1 min. Groups 1, 3, and 5 represent 2-day biofilms, and Groups 2, 4, and 6 represent 6-day biofilms. Treatments were repeated daily for 5 days. Antibacterial efficacy was analyzed by measuring oral biofilms’ red fluorescence intensity (RatioR/G) and quantifying EPS content and bacterial viability. The RatioR/G was 1.089-fold and 1.104-fold higher in Groups 4 and 6 than in Groups 3 and 5 following antibacterial treatment, respectively (p < 0.001). EPS content increased by 1.71-fold in Group 6 than in Group 5 (p < 0.001). Bacterial survival rate was the lowest in Group 3 (p = 0.005). These findings underscore the relevance of CAP treatment in maintaining antibacterial efficacy regardless of the biofilm development stage, highlighting its potential utility in oral care.
Current Applications of Artificial Intelligence in the Neonatal Intensive Care Unit
Authors: Rallis Dimitrios , Baltogianni Maria , Kapetaniou Konstantina , Giapros Vasileios
Published: May 2024

Artificial intelligence (AI) refers to computer algorithms that replicate the cognitive function of humans. Machine learning is widely applicable using structured and unstructured data, while deep learning is derived from the neural networks of the human brain that process and interpret information. During the last decades, AI has been introduced in several aspects of healthcare. In this review, we aim to present the current application of AI in the neonatal intensive care unit. AI-based models have been applied to neurocritical care, including automated seizure detection algorithms and electroencephalogram-based hypoxic-ischemic encephalopathy severity grading systems. Moreover, AI models evaluating magnetic resonance imaging contributed to the progress of the evaluation of the neonatal developing brain and the understanding of how prenatal events affect both structural and functional network topologies. Furthermore, AI algorithms have been applied to predict the development of bronchopulmonary dysplasia and assess the extubation readiness of preterm neonates. Automated models have been also used for the detection of retinopathy of prematurity and the need for treatment. Among others, AI algorithms have been utilized for the detection of sepsis, the need for patent ductus arteriosus treatment, the evaluation of jaundice, and the detection of gastrointestinal morbidities. Finally, AI prediction models have been constructed for the evaluation of the neurodevelopmental outcome and the overall mortality of neonates. Although the application of AI in neonatology is encouraging, further research in AI models is warranted in the future including retraining clinical trials, validating the outcomes, and addressing serious ethics issues.
A Smartphone-Based Algorithm for L Test Subtask Segmentation
Authors: McCreath Frangakis Alexis L. , Lemaire Edward D. , Baddour Natalie
Published: May 2024

Background: Subtask segmentation can provide useful information from clinical tests, allowing clinicians to better assess a patient’s mobility status. A new smartphone-based algorithm was developed to segment the L Test of functional mobility into stand-up, sit-down, and turn subtasks. Methods: Twenty-one able-bodied participants each completed five L Test trials, with a smartphone attached to their posterior pelvis. The smartphone used a custom-designed application that collected linear acceleration, gyroscope, and magnetometer data, which were then put into a threshold-based algorithm for subtask segmentation. Results: The algorithm produced good results (>97% accuracy, >98% specificity, >74% sensitivity) for all subtasks. Conclusions: These results were a substantial improvement compared with previously published results for the L Test, as well as similar functional mobility tests. This smartphone-based approach is an accessible method for providing useful metrics from the L Test that can lead to better clinical decision-making.
A Novel Approach to Combinatorial Problems: Binary Growth Optimizer Algorithm
Authors: Leiva Dante , Ramos-Tapia Benjamín , Crawford Broderick , Soto Ricardo , Cisternas-Caneo Felipe
Published: May 2024

The set-covering problem aims to find the smallest possible set of subsets that cover all the elements of a larger set. The difficulty of solving the set-covering problem increases as the number of elements and sets grows, making it a complex problem for which traditional integer programming solutions may become inefficient in real-life instances. Given this complexity, various metaheuristics have been successfully applied to solve the set-covering problem and related issues. This study introduces, implements, and analyzes a novel metaheuristic inspired by the well-established Growth Optimizer algorithm. Drawing insights from human behavioral patterns, this approach has shown promise in optimizing complex problems in continuous domains, where experimental results demonstrate the effectiveness and competitiveness of the metaheuristic compared to other strategies. The Growth Optimizer algorithm is modified and adapted to the realm of binary optimization for solving the set-covering problem, resulting in the creation of the Binary Growth Optimizer algorithm. Upon the implementation and analysis of its outcomes, the findings illustrate its capability to achieve competitive and efficient solutions in terms of resolution time and result quality.
Induction of Hepatoma Cell Pyroptosis by Endogenous Lipid Geranylgeranoic Acid—A Comparison with Palmitic Acid and Retinoic Acid
Authors: Shidoji Yoshihiro
Published: May 2024

Research on retinoid-based cancer prevention, spurred by the effects of vitamin A deficiency on gastric cancer and subsequent clinical studies on digestive tract cancer, unveils novel avenues for chemoprevention. Acyclic retinoids like 4,5-didehydrogeranylgeranoic acid (4,5-didehydroGGA) have emerged as potent agents against hepatocellular carcinoma (HCC), distinct from natural retinoids such as all-trans retinoic acid (ATRA). Mechanistic studies reveal GGA’s unique induction of pyroptosis, a rapid cell death pathway, in HCC cells. GGA triggers mitochondrial superoxide hyperproduction and ER stress responses through Toll-like receptor 4 (TLR4) signaling and modulates autophagy, ultimately activating pyroptotic cell death in HCC cells. Unlike ATRA-induced apoptosis, GGA and palmitic acid (PA) induce pyroptosis, underscoring their distinct mechanisms. While all three fatty acids evoke mitochondrial dysfunction and ER stress responses, GGA and PA inhibit autophagy, leading to incomplete autophagic responses and pyroptosis, whereas ATRA promotes autophagic flux. In vivo experiments demonstrate GGA’s potential as an anti-oncometabolite, inducing cell death selectively in tumor cells and thus suppressing liver cancer development. This review provides a comprehensive overview of the molecular mechanisms underlying GGA’s anti-HCC effects and underscores its promising role in cancer prevention, highlighting its importance in HCC prevention.
Senotherapeutic Peptide 14 Suppresses Th1 and M1 Human T Cell and Monocyte Subsets In Vitro
Authors: Alencar-Silva Thuany , Barcelos Stefhani Martins de , Silva-Carvalho Amandda , Sousa Mauricio Gonçalves da Costa , Rezende Taia Maria Berto , Pogue Robert , Saldanha-Araújo Felipe , Franco Octávio Luiz , Boroni Mariana , Zonari Alessandra , Carvalho Juliana Lott
Published: May 2024

Inflammation contributes to the onset and exacerbation of numerous age-related diseases, often manifesting as a chronic condition during aging. Given that cellular senescence fosters local and systemic inflammation, senotherapeutic interventions could potentially aid in managing or even reducing inflammation. Here, we investigated the immunomodulatory effects of the senotherapeutic Peptide 14 (Pep 14) in human peripheral blood mononuclear cells (PBMCs), monocytes, and macrophages. We found that, despite failing to significantly influence T cell activation and proliferation, the peptide promoted a Th2/Treg gene expression and cytokine signature in PBMCs, characterized by increased expression of the transcription factors GATA3 and FOXP3, as well as the cytokines IL-4 and IL-10. These observations were partially confirmed through ELISA, in which we observed increased IL-10 release by resting and PHA-stimulated PBMCs. In monocytes from the U-937 cell line, Pep 14 induced apoptosis in lipopolysaccharide (LPS)-stimulated cells and upregulated IL-10 expression. Furthermore, Pep 14 prevented LPS-induced activation and promoted an M2-like polarization in U-937-derived macrophages, evidenced by decreased expression of M1 markers and increased expression of M2 markers. We also showed that the conditioned media from Pep 14-treated macrophages enhanced fibroblast migration, indicative of a functional M2 phenotype. Taken together, our findings suggest that Pep 14 modulates immune cell function towards an anti-inflammatory and regenerative phenotype, highlighting its potential as a therapeutic intervention to alleviate immunosenescence-associated dysregulation.
SARS-CoV-2-Induced Type I Interferon Signaling Dysregulation in Olfactory Networks Implications for Alzheimer’s Disease
Authors: Vavougios George D. , Mavridis Theodoros , Doskas Triantafyllos , Papaggeli Olga , Foka Pelagia , Hadjigeorgiou Georgios
Published: May 2024

Type I interferon signaling (IFN-I) perturbations are major drivers of COVID-19. Dysregulated IFN-I in the brain, however, has been linked to both reduced cognitive resilience and neurodegenerative diseases such as Alzheimer’s. Previous works from our group have proposed a model where peripheral induction of IFN-I may be relayed to the CNS, even in the absence of fulminant infection. The aim of our study was to identify significantly enriched IFN-I signatures and genes along the transolfactory route, utilizing published datasets of the nasal mucosa and olfactory bulb amygdala transcriptomes of COVID-19 patients. We furthermore sought to identify these IFN-I signature gene networks associated with Alzheimer’s disease pathology and risk. Gene expression data involving the nasal epithelium, olfactory bulb, and amygdala of COVID-19 patients and transcriptomic data from Alzheimer’s disease patients were scrutinized for enriched Type I interferon pathways. Gene set enrichment analyses and gene–Venn approaches were used to determine genes in IFN-I enriched signatures. The Agora web resource was used to identify genes in IFN-I signatures associated with Alzheimer’s disease risk based on its aggregated multi-omic data. For all analyses, false discovery rates (FDR) <0.05 were considered statistically significant. Pathways associated with type I interferon signaling were found in all samples tested. Each type I interferon signature was enriched by IFITM and OAS family genes. A 14-gene signature was associated with COVID-19 CNS and the response to Alzheimer’s disease pathology, whereas nine genes were associated with increased risk for Alzheimer’s disease based on Agora. Our study provides further support to a type I interferon signaling dysregulation along the extended olfactory network as reconstructed herein, ranging from the nasal epithelium and extending to the amygdala. We furthermore identify the 14 genes implicated in this dysregulated pathway with Alzheimer’s disease pathology, among which HLA-C, HLA-B, HLA-A, PSMB8, IFITM3, HLA-E, IFITM1, OAS2, and MX1 as genes with associated conferring increased risk for the latter. Further research into its druggability by IFNb therapeutics may be warranted.
Contact Dermatitis Due to Hair Care Products: A Comprehensive Review
Authors: Cebolla-Verdugo Marta , Velasco-Amador Juan Pablo , Navarro-Triviño Francisco José
Published: May 2024

Hair cosmetics are in fashion. Numerous products are commonly used in combination. Frequent allergens are present in hair cosmetics, which, with almost daily use, increase the risk of sensitization. Familiarity with the clinical distribution of the eczematous rash is necessary to seriously consider patch testing. Not all the products on the market are haptens. It is necessary to know how to patch your own products. When avoidance of the allergen is not possible, new treatments are evaluated to combat allergic contact dermatitis, mainly of the occupational type. In this manuscript, a complete and practical review of the main allergens and contact sources of hair cosmetic origin has been carried out.
A Provably Secure Anonymous Authentication Protocol for Consumer and Service Provider Information Transmissions in Smart Grids
Authors: Ali Zahraa Abdullah , Abduljabbar Zaid Ameen , AL-Asadi Hamid Ali Abed , Nyangaresi Vincent Omollo , Abduljaleel Iman Qays , Aldarwish Abdulla J. Y.
Published: May 2024

Smart grids integrate information technology, decision support systems, communication networks, and sensing technologies. All these components cooperate to facilitate dynamic power adjustments based on received client consumption reports. Although this brings forth energy efficiency, the transmission of sensitive data over the public internet exposes these networks to numerous attacks. To this end, numerous security solutions have been presented recently. Most of these techniques deploy conventional cryptographic systems such as public key infrastructure, blockchains, and physically unclonable functions that have either performance or security issues. In this paper, a fairly efficient authentication scheme is developed and analyzed. Its formal security analysis is carried out using the Burrows–Abadi–Needham (BAN) logic, which shows that the session key negotiated is provably secure. We also execute a semantic security analysis of this protocol to demonstrate that it can resist typical smart grid attacks such as privileged insider, guessing, eavesdropping, and ephemeral secret leakages. Moreover, it has the lowest amount of computation costs and relatively lower communication overheads as well as storage costs.
Exceptional In Situ Preservation of Chondrocranial Elements in a Coniacian Mosasaurid from Colombia
Authors: Páramo-Fonseca María Eurídice , Narváez-Rincón José Alejandro , Benavides-Cabra Cristian David , Yanez-Leaño Christian Felipe
Published: May 2024

The first record of well-preserved chondrocranial elements in mosasaurids is here described. These elements are preserved in situ in a Coniacian skull found in north-central Colombia, inside a calcareous concretion. Based on a 3D model generated from computed tomography scans, we identified elements of the nasal and orbitotemporal regions. Our descriptions show that in this specimen, the chondrocranium was reduced, more so than in most lacertilians (including their closest recent relatives, the varanids), but not as severely as in snakes or amphisbaenians (which have an extremely reduced chondrocranium and limbs). The new evidence suggests that the reduction in the chondrocranium in mosasaurids could be related to modification of their limbs when adapting to aquatic environments, but also that in mosasaurids, the olfactory tract was reduced, and the optic muscle insertions occurred mainly in the interorbital septum. The exceptional preservation of the chondrocranial elements in the specimen is facilitated by a gray mineralization covering them. XRD analysis and thin section observations indicated that this mineralization is composed of microcrystalline quartz and calcite. We infer that this material was produced by a partial silicification process promoted by lower pH microenvironments associated with bacterial breakdown of non-biomineralized tissues during early diagenesis.
Fuzzy PID Control Design of Mining Electric Locomotive Based on Permanent Magnet Synchronous Motor
Authors: Ma Chi , Huang Baosheng , Basher Md Khairul , Rob Md Abdur , Jiang Yuqiang
Published: May 2024

Achieving precise stopping of electric locomotives is crucial for the realization of intelligent and unmanned auxiliary transportation systems. Presently, human drivers play a central role in ensuring accurate stopping, presenting obstacles to automation and cargo location precision, especially within the coal mining sector. This article centers on achieving the precise stopping of electric locomotives under various conditions through the utilization of permanent magnet synchronous motor-driven locomotives. This approach introduces a novel stopping control method that integrates a fuzzy proportional–integral–derivative (F-PID) controller with a vector control model for permanent magnet synchronous motors (PMSM). Subsequently, we develop the F-PID controller using the PMSM technique, incorporating new fuzzy rules for each subsystem to enhance control accuracy and efficiency. Finally, extensive simulations and real-world experiments are conducted on an electric locomotive stopping test bed to validate the effectiveness of the proposed control method. The results show that the method consistently achieves precise stopping under diverse working conditions, with an error of less than 0.3 m, confirming its robustness and reliability.
Outdoor-to-Indoor mmWave Relaying with Massive MIMO: Impact of Imperfect Channel Estimation
Authors: Bounouader Nawal , Chafnaji Houda , Benjillali Mustapha
Published: May 2024

Assuming incomplete knowledge of the channel state information (CSI), we investigate two scenarios involving millimeter wave (mmWave) relaying to support outdoor-to-indoor communications. We proceed to derive the average signal-to-noise ratio (SNR) expressions for two relaying scenarios and quantify the asymptotic SNR. The performance of the two relaying scenarios is evaluated using the outage probability—for which we have derived closed-form equations—the end-to-end channel capacity, and the energy efficiency. The obtained results are compared with those derived assuming complete knowledge of the CSI. The effect of the imperfect CSI is therefore assessed in relation to the reference of perfect CSI. In these scenarios, an outside base station (BS) in an urban cellular network serves several indoor users. In the context of a two-hop full-duplex (FD) relaying scheme, we initially suggest a method in which the base station (BS) utilizes zero-forcing (ZF) precoding, and we take into account the overall channel response. Furthermore, we make the assumption that the base station (BS) engages in precoding only depending on the response of the channel in the first hop; in this second design, the relay precodes (using the response of the second-hop channel), amplifies, and sends the signals. Both techniques utilize massive multiple-input–multiple-output (mMIMO) arrays to permit transmission. We also present Monte Carlo simulation results to assess the accuracy of our analytical results. Finally, the two systems are compared in terms of channel estimation and precoding complexity, the number of antennas, as well as the number of users. Practical deployment recommendations are formulated at the end of this work.
Hidden Variable Models in Text Classification and Sentiment Analysis
Authors: Koochemeshkian Pantea , Ihou Koffi Eddy , Bouguila Nizar
Published: May 2024

In this paper, we are proposing extensions to the multinomial principal component analysis (MPCA) framework, which is a Dirichlet (Dir)-based model widely used in text document analysis. The MPCA is a discrete analogue to the standard PCA (it operates on continuous data using Gaussian distributions). With the extensive use of count data in modeling nowadays, the current limitations of the Dir prior (independent assumption within its components and very restricted covariance structure) tend to prevent efficient processing. As a result, we are proposing some alternatives with flexible priors such as generalized Dirichlet (GD) and Beta-Liouville (BL), leading to GDMPCA and BLMPCA models, respectively. Besides using these priors as they generalize the Dir, importantly, we also implement a deterministic method that uses variational Bayesian inference for the fast convergence of the proposed algorithms. Additionally, we use collapsed Gibbs sampling to estimate the model parameters, providing a computationally efficient method for inference. These two variational models offer higher flexibility while assigning each observation to a distinct cluster. We create several multitopic models and evaluate their strengths and weaknesses using real-world applications such as text classification and sentiment analysis.
An Overview of Phase Change Materials and Their Applications in Pavement
Authors: Korniejenko Kinga , Nykiel Marek , Choinska Marta , Jexembayeva Assel , Konkanov Marat , Aruova Lyazat
Published: May 2024

The composite of a phase change material (PCM) and bitumen or asphalt as a matrix is expected as a new, advanced material for road construction. The main motivation for this article was to show the new possibilities and perspectives of developing the pavement with the usage of PCMs. Incorporating PCMs into paving materials can improve their properties, including allowing the regulation of the pavement temperature, enhancement of the pavement durability, and avoiding the phenomenon of a heat-island on the road. The main purpose of this article was to evaluate contemporary investigations in the area of the application of PCMs in pavement materials, especially asphalt and bitumen; to summarize the advantages and disadvantages of the implementation of PCM for road construction; and to discuss further trends in this area. This manuscript explored the state of the art in this area based on research in the literature. It shows the possible material solutions, presenting their composition and discussing their key properties and the manufacturing technologies used. The possibilities for further implementations are considered, especially economic issues.
Practical Experiments with a Ready-Made Strategy for Energizing a Suitable Pre-Magnetized Three-Column Three-Phase Dy Transformer in Unloaded State for Inrush Current Computations
Authors: Łukaniszyn Marian , Majka Łukasz , Baron Bernard , Sowa Marcin , Tomczewski Krzysztof , Wróbel Krzysztof
Published: May 2024

This article presents the results of an experimental verification of three-phase Dy transformer dynamics under no-load conditions. This study is motivated by previous ferroresonance analyses where the occurrence of inrush currents has been observed. The measurements covered all available electrical quantities in a transient state (12 measured and 3 additionally computed waveforms) during the device’s start-up under no-load conditions, as well as in a long-term steady state. A detailed analytical analysis is carried out for the obtained comprehensive set of measurement results. As a result of the conducted research, the mathematical model of the pre-magnetized three-phase Dy transformer is modified. Particular attention is paid to the issue of residual magnetism of the transformer core and its consideration in further research. The original strategy for energizing a three-column three-phase Dy transformer with a suitable pre-magnetization of its columns and original control switching system with a given/set value of the initial phase in the supply voltage is put to the test. The evolution of the induced inrush phenomenon up to the quasi-steady state under given (forced) conditions is documented (currents, voltages and the dynamics of changes taking place in the core (hysteresis loops)). This article represents a continuation of ongoing work on the study of transient states (dynamics of transformer inrush currents). At present, the Dy three-phase transformer is analyzed because of the requirements of industrial operators.
Biohydrogen Production under Aerial Conditions by a Nitrogen-Fixing Bacterium Isolated from a Steel Signboard
Authors: Aburai Nobuhiro , Tanaka Honami , Kohira Hana , Sekine Tinami
Published: May 2024

Hydrogen gas is attractive as a clean fuel source if it can be produced efficiently without relying on fossil fuels. Biohydrogen production using photosynthetic bacteria may enable environmentally friendly hydrogen production but is currently limited by factors such as low oxygen tolerance. In this study, we isolate a new strain of bacteria that can produce hydrogen under aerial-phase conditions compared with those under liquid-phase conditions in a nitrogen gas or an argon gas atmosphere. Bacterial strains were cultured from scrapings taken from a steel signboard. Investigation of the hydrogen production of the strains under aerial- and liquid-phase conditions and subsequent DNA sequencing led to identification of the bacterium Cereibacter sp. KGU-NF001. Aerial-phase conditions were achieved by filter membranes with the bacterial strains and placing the membranes on medium-soaked cotton wool. The gas atmosphere affected the behavior of the isolated bacterial strains under both aerial- and liquid-phase conditions. Cereibacter sp. KGU-NF001 showed promising oxygen tolerance and was able to maintain hydrogen production of 1.33 mL/mg/d even when the atmosphere contained 12% oxygen. Our findings illustrate that biohydrogen production may be achieved by photosynthetic bacteria under oxygen-containing aerial-phase conditions, indicating a possible pathway to help lower our reliance on fossil fuels.
Transcriptome Analysis of Sake Yeast in Co-Culture with kuratsuki Kocuria
Authors: Kobayashi Karin , Nishida Hiromi
Published: May 2024

Kuratsuki bacteria enter the sake production process and affect the flavor and taste of sake. This study compared gene expression in the sake yeast Saccharomyces cerevisiae in co-culture with kuratsuki Kocuria to that in monoculture. Among the 5922 genes of S. cerevisiae, 71 genes were upregulated more than 2-fold, and 61 genes were downregulated less than 0.5-fold in co-culture with kuratsuki Kocuria. Among the stress-induced genes, fourteen were upregulated, and six were downregulated. Among the fourteen upregulated genes, six were induced in response to replication stress. Although the G1 cyclin gene CLN3 was upregulated by more than 2-fold, eight genes that were induced in response to meiosis and/or sporulation were also upregulated. Fourteen metabolism-related genes, for example, the glyceraldehyde-3-phosphate dehydrogenase genes TDH1, TDH2, and TDH3, were downregulated by less than 0.5-fold in co-culture with kuratsuki Kocuria. The gene expression patterns of S. cerevisiae co-cultured with kuratsuki Kocuria differed from those co-cultured with lactic acid bacteria. Therefore, S. cerevisiae responded differently to different bacterial species. This strongly suggests that kuratsuki bacteria affect gene expression in sake yeast, thereby affecting the flavor and taste of sake.
Valorization of Cashew Apple Waste into a Low-Alcohol, Healthy Drink Using a Co-Culture of Cyberlindnera rhodanensis DK and Lactobacillus pentosus A14-6
Authors: Kham Nang Nwet Noon , Phovisay Somsay , Unban Kridsada , Kanpiengjai Apinun , Saenjum Chalermpong , Lumyong Saisamorn , Shetty Kalidas , Khanongnuch Chartchai
Published: May 2024

This study investigated the potential of microbial fermentative transforming processes in valorizing the cashew apple by-product into a low-alcohol, health-benefiting beverage. We particularly investigated the use of a non-Saccharomyces yeast, Cyberlindnera rhodanensis DK, as the main targeted microbe. At 30 °C without agitation, C. rhodanensis DK caused changes in key parameters during the fermentation of cashew apple juice (CAJ) in terms of varied pH values and initial sugar concentrations. This result indicated that pure CAJ, with pH adjusted to 6 and with the original 6.85% (w/v) total sugar content, was the most feasible condition, as glucose and fructose were mostly consumed at 12 days of fermentation. A co-culture approach with either Saccharomyces cerevisiae TISTR 5088 or Lactobacillus pentosus A14-6 was investigated to improve both physicochemical and fermentation characteristics. Co-fermentation with S. cerevisiae TISTR 5088 resulted in significantly increased ethanol accumulation to 33.61 ± 0.11 g/L, but diminished bioactive compounds, antioxidant activity, and antidiabetic potential. In contrast, co-fermentation with L. pentosus A14-6 demonstrated excellent outcomes, as it significantly increased sugar consumption and finally remained at only 4.95 g/L compared to C. rhodanensis DK alone, produced lower levels of ethanol at only 19.47 ± 0.06 g/L, and higher total titratable acid (TTA), resulting in a final pH of 3.6. In addition, co-fermentation with this lactic acid bacterium significantly enhanced bioactive compounds and antioxidant activity and also retained potential antidiabetic properties. These findings highlight the feasibility of using tailored microbial fermentation strategies to produce low-alcohol beverages with enhanced health-promoting properties from CAJ; however, product-development processes following health food regulations and sensory evaluation are necessary.
Inhibition of LPS-Induced Skin Inflammatory Response and Barrier Damage via MAPK/NF-κB Signaling Pathway by Houttuynia cordata Thunb Fermentation Broth
Authors: Song Zixin , Fang Jiaxuan , Wang Dongdong , Tian Yuncai , Xu Yuhua , Wang Ziwen , Geng Jiman , Wang Changtao , Li Meng
Published: May 2024

Houttuynia cordata Thunb is rich in active substances and has excellent antioxidant and anti-inflammatory activity. Scanning electron microscopy and gel permeation chromatography were used to analyze the molecular characteristics of the fermentation broth of Houttuynia cordata Thunb obtained through fermentation with Clavispora lusitaniae (HCT-f). The molecular weight of HCT-f was 2.64265 × 105 Da, and the polydispersity coefficient was 183.10, which were higher than that of unfermented broth of Houttuynia cordata Thunb (HCT). By investigating the active substance content and in vitro antioxidant activity of HCT-f and HCT, the results indicated that HCT-f had a higher active substance content and exhibited a superior scavenging effect on 2,2-diphenyl-1-picrylhydrazyl radicals and hydroxyl radicals, with IC50 values of 11.85% and 9.01%, respectively. Our results showed that HCT-f could effectively alleviate the increase in the secretion of inflammatory factors and apoptotic factors caused by lipopolysaccharide (LPS) stimulation, and had a certain effect on repairing skin barrier damage. HCT-f could exert an anti-inflammatory effect by down-regulating signaling in the MAPK/NF-κB pathway. The results of erythrocyte hemolysis and chicken embryo experiments showed that HCT-f had a high safety profile. Therefore, this study provides a theoretical basis for the application of HCT-f as an effective ingredient in food and cosmetics.
Doenjang Ameliorates Diet-Induced Hyperlipidemia and Hepatic Oxidative Damage by Improving Lipid Metabolism, Oxidative Stress, and Inflammation in ICR Mice
Authors: Edward Olivet Chiamaka , Jeong Do-Youn , Yang Hee-Jong , Han Anna , Cha Youn-Soo
Published: May 2024

Hyperlipidemia, characterized by elevated cholesterol, lipids, and triglycerides in the bloodstream, is linked to hepatic oxidative damage. Doenjang, a traditional Korean condiment made from fermented soybeans, is known for its health benefits, yet its anti-hyperlipidemic effects remain understudied. Our study aimed to assess the hypolipidemic and hepatic protective effects of Doenjang on male ICR mice fed a high-fat cholesterol diet for 8 weeks. Mice were divided into three groups: the normal diet (ND), the high-fat cholesterol diet (HD), and the Doenjang-supplemented HD diet (DS) group. Doenjang supplementation significantly regulated total cholesterol, triglycerides, LDL cholesterol, and HDL cholesterol levels compared to the HD group. It also downregulated lipogenic genes, including PPARγ, FAS, and ACC, and positively influenced the cholesterol metabolism-related genes HMGCR and LXR. Moreover, Doenjang intake increased serum glutathione levels, activated oxidative stress defense genes (NRF2, SOD, GPx1, and CAT), positively modulated inflammation genes (NF-kB and IL6) in hepatic tissue, and reduced malondialdehyde levels. Our findings highlight the effectiveness of traditional Doenjang in preventing diet-induced hyperlipidemia and protecting against hepatic oxidative damage.
Quality Changes and Fungal Microbiota Dynamics in Stored Jujube Fruits: Insights from High-Throughput Sequencing for Food Preservation
Authors: Zhao Lili , Li Hongbo , Liu Zhenbin , Hu Liangbin , Xu Dan , Zhu Xiaolin , Mo Haizhen
Published: May 2024

Postharvest rot is an urgent problem affecting the storage of winter jujube. Therefore, the development of new technologies for efficient and safe preservation is very important. This study aimed to elucidate the fungal microbiota found on the epidermis of jujube during the storage period using high-throughput sequencing, as well as to monitor the changes in quality indexes throughout this period. Through internal transcribed spacer (ITS) sequencing, we identified two phyla (Basidiomycota and Ascomycota) and six genera (Cryptococcus, Bulleromyces, Sporidiobolus, Alternaria, Pseudozyma, and Sporobolomyces), which potentially contribute to the spoilage and deterioration of jujube, referred to as “core fungal taxa”. A high correlation was further found between preservation indices (including decay rate, firmness, and total soluble solids) and the growth of multiple core fungi over time. These findings will provide insights and a theoretical basis for further research on preservation techniques related to biological control during date fruit storage.
An Autoethnography on Intergenerational Relationships and Transnational Care for Older Parents
Authors: Zhang Weiguo
Published: May 2024

I employ autoethnography to undertake a broader scholarly inquiry on intergenerational relationships and transnational care shaped by global migration and aging. Specifically, I reflect on the dynamics of my relationship with my mother, beginning with my departure from my home and spanning a period of 40 years, 8 in China and 34 outside China. In doing so, I contemplate theoretical models of intergenerational solidarity, ambivalence, and role ambiguity. I also challenge cultural assumptions of filial piety. The geographical distance, passage of time, and acculturation process have profoundly influenced my perception of filial piety, which differs markedly from my mother’s. However, this divergence in consensual solidarity—marked by variations in attitudes, beliefs, and values—does not translate into weakened affectual solidarity, characterized by positive sentiments and emotions. Furthermore, aided by advancements in transportation and social media technology, I have been able to extend crucial emotional and some “instrumental” care to my mother, along with financial support if needed, despite limited hands-on care. Nevertheless, I must negotiate my care for my mother and navigate a delicate balance in coordinating my care efforts with those of my non-migrant siblings.