bluuebunny/crossref_metadata_2025 huggingface dataset:
Numerical modeling and experimental validation of fluid flow in micro- and meso-fluidic siphons
Authors: Akhras Nour , Singh Gurjas , Gill Kirandeep K. , Bola Shaan , Al-Hakeem Kareem , Reis Nuno M.
Published: August 2024

Siphons have been used for thousands of years to transfer fluids without the use of pumps or power and are present in our daily lives. Paradoxically, it is only in recent decades that the operation of siphons has been fully clarified, which is now understood to be exclusively linked to gravity and molecular cohesion. Siphons are uniquely able to offer automatic, intermittent flow, yet present the main drawback of requiring a source of energy to induce initial flow. Our research team has recently disclosed a microfluidic siphon able to self-prime and deliver a sequence of bioanalytical reagents, previously demonstrated for high-performance, multi-reagents diagnostic testing. Here we show for the first time 2D and 3D computational fluid dynamics (CFD) modeling and the experimental characterization of fluid flow in a range of miniaturized hydrophilic siphons of varying hydraulic liquid height-to-length ratios, ΔH/LT = 0–0.9, using fluids of varying viscosities. CFD simulations using velocity- and pressure-driven inlet boundary conditions were generally in good agreement with experimental fluid flow rates and pressure-balance predictions for plastic ∼0.2 mm and glass ∼0.6 mm internal diameter microfluidic siphons. CFD predictions of fluid flow in “meso-scale” siphons with 1 and 2 mm internal diameters also fully matched normalized experimental data, suggesting that miniaturized siphons are scalable. Their discharge rate and pressure drop are readily predicted and fine-tunable through the physical properties of the fluid and some design parameters of the siphon. The wide range of experimental and numerical parameters studied here provide an important framework for the design and application of gravity-driven micro- and meso-fluidic siphons in many applications, including but not limited to life sciences, clinical diagnostics, and process intensification.
Guanidinium and spermidinium decavanadates: as small biomimetic models to understand non-covalent interactions between decavanadate and arginine and lysine side chains in proteins
Authors: Paredes-Pérez Luis F. , Mendoza Angel , García-García Amalia , Serrano-De la Rosa Laura E. , Méndez-Rojas Miguel A. , Melendez Francisco J. , Castro María Eugenia , González-Vergara Enrique
Published: August 2024

During the last three decades, numerous investigations have been conducted on polyoxidovanadates to treat several illnesses and inhibit enzymes. Numerous decavanadate compounds have been proposed as potential therapies for Diabetes mellitus, Cancer, and Alzheimer’s disease. Only six relevant functional proteins interacting with decavanadate, V10, have been deposited in the PDB. These are acid phosphatase, tyrosine kinase, two ecto-nucleoside triphosphate diphosphohydrolases (NTPDases), the human transient receptor potential cation channel (TRPM4), and the human cell cycle protein CksHs1. The interaction sites in these proteins mainly consist of Arginine and Lysine, side chains binding to the decavanadate anion. To get further knowledge regarding non-covalent interactions of decavanadate in protein environments, guanidinium and spermidinium decavanadates were synthesized, crystallized, and subjected to analysis utilizing various techniques, including FTIR, Raman, 51V-NMR, TGA, and X-ray diffraction. The DFT calculations were employed to calculate the interaction energy between the decavanadate anion and the organic counterions. Furthermore, the Quantum Theory of Atoms in Molecules (QTAIM) and Non-covalent Interaction-Reduced Density Gradient (NCI-RDG) analyses were conducted to understand the non-covalent interactions present in these adducts. Decavanadate can engage in electrostatic forces, van der Waals, and hydrogen bond interactions with guanidinium and spermidinium, as shown by their respective interaction energies. Both compounds were highly stabilized by strong hydrogen bond interactions N−H···O and weak non-covalent interactions C−H···O. In addition, the interactions between guanidinium and spermidinium cations and decavanadate anion form several stable rings. This study provides new information on non-covalent intermolecular interactions between decavanadate and small biomimetic models of arginine and lysine lateral chains in protein environments.
Preparation and application of single-atom nanozymes in oncology: a review
Authors: Liang Huiyuan , Xian Yijie , Wang Xujing
Published: August 2024

Single-atom nanozymes (SAzymes) represent a cutting-edge advancement in nanomaterials, merging the high catalytic efficiency of natural enzymes with the benefits of atomic economy. Traditionally, natural enzymes exhibit high specificity and efficiency, but their stability are limited by environmental conditions and production costs. Here we show that SAzymes, with their large specific surface area and high atomic utilization, achieve superior catalytic activity. However, their high dispersibility poses stability challenges. Our review focuses on recent structural and preparative advancements aimed at enhancing the catalytic specificity and stability of SAzymes. Compared to previous nanozymes, SAzymes demonstrate significantly improved performance in biomedical applications, particularly in tumor medicine. This progress positions SAzymes as a promising tool for future cancer treatment strategies, integrating the robustness of inorganic materials with the specificity of biological systems. The development and application of SAzymes could revolutionize the field of biocatalysis, offering a stable, cost-effective alternative to natural enzymes.
Mo3P/Mo heterojunction for efficient conversion of lithium polysulfides in high-performance lithium-sulfur batteries
Authors: Sun Zhongpeng , Wang Yuanhao , Xu Jie , Wang Xia
Published: August 2024

Realizing efficient immobilization of lithium polysulfides (LiPSs) as well as reversible catalytic conversion between LiPSs and the insoluble Li2S is vital to restrain the shuttle effect, which requires highly reactive catalysts for high-performance Li-S batteries. Here, three-dimensional ordered porous Mo-based metal phosphides (3DOP Mo3P/Mo) with heterogeneous structures were fabricated and utilized as separator-modified coatings for Li-S batteries to catalyze the conversion of LiPSs. The adsorption, catalytic and electrochemical performance of the corresponding cells were compared among 3DOP Mo3P/Mo and 3DOP Mo, by kinetic and electrochemical performance measurements. It was found that the cell with 3DOP Mo3P/Mo modified separator deliver better electrochemical performance, with a high specific capacity of 469.66 mAh g−1 after 500 cycles at a high current density of 1°C. This work provides an idea and a guideline for the design of the separator modification for high-performance Li-S batteries.
The safety and efficacy of third- and fourth-generation cryoballoons for atrial fibrillation: a systematic review and meta-analysis
Authors: Zhang Man-Li , Zhang Chao , Peng Jian-Yong , Xing Shu-Qiao , Guo Jian , Wei Chen-Long , Zhang Neng-Fang , Ma En , Chen Wen-Sheng
Published: August 2024

Objectives

An increasing number of studies have shown that third (CB3)- and fourth-generation cryoballoons (CB4) have been used to treat various types of atrial fibrillation (AF), but previous research regarding the safety and efficacy of CB3 or CB4 ablation remains controversial. Therefore, a meta-analysis was performed to further evaluate the safety and efficacy of pulmonary vein isolation (PVI) using the CB3 and CB4 in the treatment of AF.

Methods

We searched PubMed, the Cochrane Library, Web of Science, China National Knowledge Infrastructure, Wanfang, China Science and Technology Journal Database, and Clinicaltrials.gov up to December 2023 for qualified trials and data extraction according to inclusion and exclusion criteria. All analyses were carried out using Review Manager 5.3 software.

Results

The meta-analysis included 13 observational studies consisting of 3,281 subjects and did not include a randomized controlled trial. Overall analyses indicated that the CB3 significantly reduced total procedure time [weighted mean difference (WMD) = −8.69 min, 95% confidence interval (CI) = −15.45 to −1.94 min, I2 = 93%], increased the PVI recording [relative risk (RR) = 1.24, 95% CI = 1.03–1.49, I2 = 90%], and increased the mean nadir temperature of overall PVs (WMD = 2.80°C, 95% CI = 1.08–4.51°C, I2 = 89%) compared with the CB2. Moreover, the CB4 significantly reduced the total procedure time (WMD = −14.50 min, 95% CI = −20.89 to −8.11 min, I2 = 95%), reduced the fluoroscopy time (WMD = −2.37 min, 95% CI = −4.28 to −0.46 min, I2 = 95%), increased the PVI recording (RR = 1.40, 95% CI = 1.15–1.71, I2 = 90%) compared with the CB2. Time-to-isolation, the success rate of PVI, AF recurrence, and complications in the CB3 and CB4 were not significantly different compared with the CB2.

Conclusion

These findings demonstrated that the CB3 and CB4 tended to be more effective than the CB2 in the treatment of AF, with shorter procedure times, more PVI recording, and similar safety endpoints.
Application of telemedicine technology for cardiovascular diseases management during the COVID-19 pandemic: a scoping review
Authors: Asadi Hassan , Toni Esmaeel , Ayatollahi Haleh
Published: August 2024

Background

Recently, the use of telemedicine technology has increased due to the Covid-19 pandemic. Cardiovascular diseases management is one of the areas that has benefited from using this technology. This study aimed to identify the applications of telemedicine for cardiovascular diseases management during the Covid-19 pandemic.

Methods

This scoping study was conducted in 2023. Various databases, including PubMed, Web of Science, Scopus, the Cochrane Library, Ovid, CINAHL, ProQuest, and IEEE Xplore along with Google Scholar were searched and all related quantitative, qualitative, and mixed-method studies published in English between 2020 and 2022 were included. Finally, the required data were extracted, and the findings were reported narratively.

Results

A total of 17 articles were included in this study. The results showed that teleconsultation via telephone and videoconferencing were the most common used technologies. Telemedicine helped to improve clinical impacts such as patient health status and quality of care, and reduced hospitalizations and re-admission rates compared to in-person visits. The non-clinical impact included reducing waiting time, in-person visits, and healthcare costs during the Covid-19 pandemic.

Conclusion

The Covid-19 pandemic has led to an increased use of telemedicine technology, especially for patients with cardiovascular diseases. As teleconsultation and telemonitoring are useful for cardiovascular diseases management and regular examinations, future research should investigate how these technologies can be improved and used for a wider population.
Potential biomarkers and immune characteristics for polycythemia vera-related atherosclerosis using bulk RNA and single-cell RNA datasets: a combined comprehensive bioinformatics and machine learning analysis
Authors: Wang Ziqing , Zou Jixuan
Published: August 2024

Background

Polycythemia vera (PV) is a myeloproliferative disease characterized by significantly higher hemoglobin levels and positivity for JAK2 mutation. Thrombosis is the main risk event of this disease. Atherosclerosis (AS) can markedly increase the risk of arterial thrombosis in patients with PV. The objectives of our study were to identify potential biomarkers for PV-related AS and to explore the molecular biological association between PV and AS.

Methods

We extracted microarray datasets from the Gene Expression Omnibus (GEO) dataset for PV and AS. Common differentially expressed genes (CGs) were identified by differential expression analysis. Functional enrichment and protein-protein interaction (PPI) networks were constructed from the CG by random forest models using LASSO regression to identify pathogenic genes and their underlying processes in PV-related AS. The expression of potential biomarkers was validated using an external dataset. A diagnostic nomogram was constructed based on potential biomarkers to predict PV-related AS, and its diagnostic performance was assessed using ROC, calibration, and decision curve analyses. Subsequently, we used single-cell gene set enrichment analysis (GSEA) to analyze the immune signaling pathways associated with potential biomarkers. We also performed immune infiltration analysis of AS with “CIBERSORT” and calculated Pearson's correlation coefficients for potential biomarkers and infiltrating immune cells. Finally, we observed the expression of potential biomarkers in immune cells based on the single-cell RNA dataset.

Results

Fifty-two CGs were identified based on the intersection between up-regulated and down-regulated genes in PV and AS. Most biological processes associated with CGs were cytokines and factors associated with chemotaxis of immune cells. The PPI analysis identified ten hub genes, and of these, CCR1 and MMP9 were selected as potential biomarkers with which to construct a diagnostic model using machine learning methods and external dataset validation. These biomarkers could regulate Toll-like signaling, NOD-like signaling, and chemokine signaling pathways associated with AS. Finally, we determined that these potential biomarkers had a strong correlation with macrophage M0 infiltration. Further, the potential biomarkers were highly expressed in macrophages from patients with AS.

Conclusion

We identified two CGs (CCR1 and MMP9) as potential biomarkers for PV-related AS and established a diagnostic model based on them. These results may provide insight for future experimental studies for the diagnosis and treatment of PV-related AS.
Learning for children in an educational enrichment: a perspective derived from Taiwan's Early Childhood Education & Care Curriculum Framework
Authors: Shih Yi-Huang
Published: August 2024

Anchored in the educational philosophy of “benevolence,” the Taiwan's Early Childhood Education & Care Curriculum Framework establishes nine learning goals and emphasizes the cultivation of six core competencies in young children. Recognizing that young children have potential for development, imagination, and creativity, the framework encourages young learners to engage with their surroundings, exhibit curiosity, and explore through play. The curriculum framework highlights the multifaceted roles of teachers, including their roles in shaping class culture and learning environments, partnering in the lives and learning journeys of children, guiding in their educational development, and collaborating with families. This curriculum framework emphasizes a child-centered approach to course development, integrating diverse activities that are reflective of and relevant to children's life experiences. It prioritizes maintaining the physical and mental health and safety of young children and introduces them to the meaning of life and the phenomenon of multiculturalism. The type of this study is a conceptual analysis, and it is a study from a reflective model on the implementation of the curriculum in Taiwan. Through this curriculum framework, children are encouraged to appreciate life's beauty and interact with others effectively and empathetically. Therefore, the curriculum framework emphasizes life education, multicultural education, aesthetic education, and moral education as essential components in the holistic development of young children. The curriculum framework is an educational enrichment for children's learning. This study explores the concept of early childhood learning as conceptualized by Taiwan's Early Childhood Education & Care Curriculum Framework. It is hoped that such an exploration can broaden young children's learning horizons, and enhance the quality of early childhood education.
Diabetic peripheral neuropathy based on Schwann cell injury: mechanisms of cell death regulation and therapeutic perspectives
Authors: Wu Lijiao , Wang Xiang Jin , Luo Xi , Zhang Jingqi , Zhao Xinyi , Chen Qiu
Published: August 2024

Diabetic peripheral neuropathy (DPN) is a complication of diabetes mellitus that lacks specific treatment, its high prevalence and disabling neuropathic pain greatly affects patients’ physical and mental health. Schwann cells (SCs) are the major glial cells of the peripheral nervous system, which play an important role in various inflammatory and metabolic neuropathies by providing nutritional support, wrapping axons and promoting repair and regeneration. Increasingly, high glucose (HG) has been found to promote the progression of DPN pathogenesis by targeting SCs death regulation, thus revealing the specific molecular process of programmed cell death (PCD) in which SCs are disrupted is an important link to gain insight into the pathogenesis of DPN. This paper is the first to review the recent progress of HG studies on apoptosis, autophagy, pyroptosis, ferroptosis and necroptosis pathways in SCs, and points out the crosstalk between various PCDs and the related therapeutic perspectives, with the aim of providing new perspectives for a deeper understanding of the mechanisms of DPN and the exploration of effective therapeutic targets.
The role of Clec11a in bone construction and remodeling
Authors: Xu Ke , Huang Rui-qi , Wen Ruiming , Yang Yao , Cheng Yang , Chang Bo
Published: August 2024

Bone is a dynamically active tissue whose health status is closely related to its construction and remodeling, and imbalances in bone homeostasis lead to a wide range of bone diseases. The sulfated glycoprotein C-type lectin structural domain family 11 member A (Clec11a) is a key factor in bone mass regulation that significantly promotes the osteogenic differentiation of bone marrow mesenchymal stem cells and osteoblasts and stimulates chondrocyte proliferation, thereby promoting longitudinal bone growth. More importantly, Clec11a has high therapeutic potential for treating various bone diseases and can enhance the therapeutic effects of the parathyroid hormone against osteoporosis. Clec11a is also involved in the stress/adaptive response of bone to exercise via mechanical stimulation of the cation channel Pieoz1. Clec11a plays an important role in promoting bone health and preventing bone disease and may represent a new target and novel drug for bone disease treatment. Therefore, this review aims to explore the role and possible mechanisms of Clec11a in the skeletal system, evaluate its value as a potential therapeutic target against bone diseases, and provide new ideas and strategies for basic research on Clec11a and preventing and treating bone disease.
Causal validation of the relationship between 35 blood and urine biomarkers and hyperthyroidism: a bidirectional Mendelian randomization study and meta-analysis
Authors: Xu Wanxian , Wu Jiao , Chen Daolei , Zhang Rui , Yang Yue
Published: August 2024

Background

Hyperthyroidism is an endocrine disorder with a relatively low global prevalence but significantly higher incidence among females compared to males. The onset age primarily ranges from 30 to 50, although it is not limited to this age group. Challenges in the treatment of hyperthyroidism include individualized treatment plan formulation, management of side effects, and prediction of disease progression, necessitating comprehensive consideration to achieve more effective therapy and management. Mendelian randomization studies can reveal more precise therapeutic targets between blood and urine biomarkers and hyperthyroidism, providing more decadent treatment options for the condition.

Methods

The study will build upon the omics Mendelian randomization (MR) framework by conducting MR analysis using 35 blood and urine biomarkers separately for two distinct databases of hyperthyroidism. Subsequently, the results will undergo meta-analysis and multiple corrections to ensure accuracy and reliability. Finally, positive findings will undergo reverse MR validation to verify causal relationships with hyperthyroidism.

Results

In the British database, the MR analysis of Total bilirubin levels about hyperthyroidism yielded an odds ratio (OR) of 1.097 (95% CI: 0.951-1.265, P = 0.205). Conversely, in the Thyroid Omics Association database, the MR analysis revealed an OR of 1.283 (95% CI: 1.122-1.467, P = 0.0002) for the same relationship. Meta-analysis of the MR analysis results from both databases, following multiple corrections, resulted in an OR of 1.192 (95% CI: 1.081-1.314, P = 0.015). Additionally, the direction of beta values in the MR analysis results from both databases was consistent.

Conclusion

The urine biomarker total bilirubin levels may contribute to an increased risk of hyperthyroidism and accelerate its progression, thus representing a risk factor for the condition.
The importance of natural land carbon sinks in modelling future emissions pathways and assessing individual country progress towards net-zero emissions targets
Authors: van der Ploeg Robin , Haigh Martin
Published: August 2024

Nature-based solutions (NBS), in the form of active ecosystem conservation, restoration and improved land management, represent a pathway to accelerate net-zero emissions (NZE) strategies and support biodiversity. Meaningful implementation and successful accounting depend on the ability to differentiate between anthropogenic and natural carbon fluxes on land. The United Nations Framework Convention on Climate Change (UNFCCC) land carbon accounting methods currently incorporate all CO2fluxes on managed land in country inventories without distinguishing between anthropogenic and natural components. Meanwhile, natural land carbon sinks are modelled by earth system models but are mostly reported at global level. Here we present a simple yet novel methodology to estimate the present and future progression of natural land sinks at the country and regional level. Forests dominate the uptake of CO2on land and as such, our analysis is based on allocating global projections of the natural land carbon flux to individual countries using a compilation of forest land areas for a historic and scenario range spanning 1960–2100. Specifically, we use MIT’s carbon cycle model simulations that are set in the context of emissions pathways from the Shell Energy Security Scenarios (2023). Our natural land carbon flux estimates for individual countries and regions such as the European Union (EU) show generally good agreement with independent estimates from recent land-use harmonisation studies for 2000–2020. Hence, our approach may also provide a simple, first-order exploration of future natural land fluxes at country level—a potential that other studies do not yet offer. In turn, this enables better understanding of the anthropogenic and natural components contributing to country NZE targets under different scenarios. Nevertheless, our findings also suggest that models such as the Shell World Energy Model (WEM) would benefit from further improvements in the apportionment of land carbon sources and sinks to evaluate detailed actions to meet country targets. More importantly, uncertainties remain regarding the resilience of land ecosystems and their capacity to store increasing amounts of carbon under progressive global warming. Therefore, we recommend that the carbon cycle modelling and energy modelling research communities continue to collaborate to develop a next generation of relevant data products to distinguish anthropogenic from natural impacts at local, regional and national levels.
Distribution characteristics of soil crusts under two kinds of aerial seeding plants in the northeastern margin of Tengger Desert
Authors: Zhang Ran , Liang Yumei , Gao Yong , Wang Yanyi
Published: August 2024

In order to explore the effects of two plants on the spatial distribution and nutrient distribution of crusts in different directions and distances after 30 years of aerial seeding, as well as the relationship between crust thickness and nutrient content, this paper takes the aerial seeding afforestation area in the northeastern margin of Tengger Desert as the research area, and the crusts under the plants of Hedysarum scoparium and Calligonum mongolicum as the research objects. The distribution of crust thickness and nutrients was analyzed, and the relationship between them was constructed. The thickness of the crust of H. scoparium and C. mongolicum was the largest in the southeast direction and the smallest in the northwest direction. With increasing distance from the plant, it shows a significant decreasing trend. The crust thickness of H. scoparium was higher than that of C. mongolicum. The nutrient indexes in the southeast direction of the crust of H. scoparium and C. mongolicum were significantly higher than those in the northwest direction; With the increase of the distance from the root of the plant, the nutrient content showed a significant decreasing trend. The contents of organic matter and available nitrogen in the crust of C. mongolicum were higher than those of H. scoparium, and the contents of available potassium and available phosphorus were lower than those of H. scoparium. The trend of nutrient enrichment rate of the crusts of the two plants was consistent with the trend of nutrient distribution. There was a significant positive correlation between the crust thickness and nutrients in the four directions under C. mongolicum. There was a significant positive correlation between the crust thickness and nutrients in the southwest direction under H. scoparium. The changes of available nitrogen, available phosphorus, available potassium and organic matter content in the crust under H. scoparium can explain 52.5% of the change of its thickness. The changes of available nitrogen, available phosphorus, available potassium and organic matter content in the crust under C. mongolicum can explain 78.5% of the change of its thickness. After 30 years of aerial seeding in Tengger Desert, the thickness and nutrients of the crust in the southeast direction of H. scoparium and C. mongolicum were significantly higher than those in the northwest direction and showed a decreasing trend with the increase of distance from the root of the plant. There was a positive correlation between crust nutrient and thickness.
Advancing sustainable agriculture: the role of integrated soil-crop management in maize production
Authors: Wang Zhan , Zhao Xiaoxiao , Sun Yongfeng , Liu Wei , Zhao Guoqiang , Dang Zhaohui
Published: August 2024

Objective

This study aims to evaluate the effectiveness of Integrated Soil-Crop System Management (ISSM) and provide technical support for sustainable high yield and efficiency in regional agriculture.

Methods

The study compared the effects of no fertilization (Control), conventional farmer practices (FP), high-yield management (HY), and ISSM on maize yield and plant nutrient uptake. Measurements included grain yield, plant biomass, plant nutrient absorption, and soil nutrient content across different management strategies.

Results

Over the 12-year experimental period, a significant decline in grain yield was observed under the Control treatment, with a slight decrease in the FP treatment. In contrast, consistent yield increases were noted for the HY and ISSM treatments. The ISSM approach significantly enhanced the average yield and plant uptake of P and K by 26%, 24%, and 32%, respectively, approaching 98%, 91%, and 85% of the levels achieved in the HY treatment. Furthermore, the average use efficiency of P and K fertilizers in the ISSM treatment exceeded those in the FP treatment by 18.7% and 1.2%, respectively, and those in the HY treatment by 17.4% and 24.8%, respectively. The adoption of ISSM led to a significant increase in total and available P and K content within the 0–20 cm and 20–40 cm soil layers and enhanced the available P and K content across all aggregate size fractions within the 0–20 cm soil layer.

Conclusion

ISSM is capable of achieving long-term high and stable yields for spring maize, enhancing the uptake and utilization of P and K in plants, and bolstering the soil’s capacity to supply these nutrients, thereby fostering the sustainable development of the entire soil-crop system.
Acute intermittent porphyria: a disease with low penetrance and high heterogeneity
Authors: Lei Jia-Jia , Li Shuang , Dong Bai-Xue , Yang Jing , Ren Yi
Published: August 2024

Acute intermittent porphyria (AIP) is caused by mutations in the gene encoding hydroxymethylbilane synthase (HMBS), a key enzyme in the heme biosynthesis pathway. AIP is an autosomal dominant disorder characterized by low penetrance and a highly heterogenous clinical presentation. The estimated prevalence of AIP is 5–10 cases per 100,000 persons, with acute attacks manifesting in less than 1% of the at-risk population. This low frequency of attacks suggests significant roles for oligogenic inheritance and environmental factors in the pathogenesis of the disease. In recent years, identification of several modifier genes has advanced our understanding of the factors influencing AIP penetrance and disease severity. This review summarizes these factors including the impact of specific HMBS mutations, oligogenic inheritance, mitochondrial DNA copy number, age, sex, the influence of sex hormones, and the role of environmental factors. Further studies into the etiology of AIP disease penetrance should inform pathogenesis, potentially allowing for the development of more precise diagnostic and therapeutic approaches.
Fahr’s syndrome as the initial imaging characteristics of MELAS syndrome with a possible seizure activity and cardiac arrest: a case report
Authors: Zheng Yan , Wu Haohao , Zhang Meng , Huang Baogang , Yang Junsu , Liu Chuan , Wang Hanmin , Du Kang
Published: August 2024

This study reported a case of MELAS syndrome presenting as the initial imaging characteristics of Fahr’s syndrome with “near” sudden unexpected death in epilepsy (SUDEP) and lateralized periodic discharges (LPD). The patient, a young boy, experienced loss of consciousness 2 days prior, which was followed by two limb and facial convulsions. He was later found in cardiac arrest during hospitalization, but regained consciousness gradually after receiving cardiopulmonary resuscitation and tracheal intubation. The patient exhibited short stature, intellectual disability, poor sports abilities, and academic performance since childhood, but had no family history. Emergency head computed tomography (CT) revealed high density calcification in bilateral caudate nucleus, lentiform nucleus, thalamus, and dentate nucleus with evidence of an acute process. The patient was transferred to the neurology department where he continued to recover consciousness, though he experienced dysarthria, left limb hemiplegia, and hemiparesthesia. Changes in head magnetic resonance imaging (MRI) findings were noted at admission, 1 month later, and 6 months later. LPD were observed in his video electroencephalogram. The CT urography indicated a narrow left ureteropelvic junction with left hydronephrosis, which was suggestive of ureteropelvic junction obstruction. Ultimately, a diagnosis of near-SUDEP was suspected in this patient, indicating a rare case of MELAS syndrome with near-SUDEP and LPD. The gene tests results revealed the presence of the mitochondrial DNA A3243G mutation, leading to the final diagnosis of MELAS syndrome. This case expands the clinical disease spectrum of the MELAS syndrome.
Spatial variation of skilled birth attendance and associated factors among reproductive age women in Ethiopia, 2019; a spatial and multilevel analysis
Authors: Aragaw Fantu Mamo , Atlie Gela , Tesfaye Amensisa Hailu , Belay Daniel Gashaneh
Published: August 2024

Background

The majority of maternal deaths were associated with a lack of access to skilled birth attendance. Because childbirth accounts for most maternal deaths, skilled birth attendance is crucial for reducing maternal mortality. The use of skilled birth attendance in Ethiopia is low, and it is crucial to identify factors that determine the use of skilled birth attendance. Hence, this study aimed to assess the spatial distribution, wealth-related inequality, and determinants for skilled birth attendance in Ethiopia.

Methods

Secondary data analysis was done with a total weighted sample of 5,251 reproductive-aged women using the 2019 mini EDHS. The concentration index and graph were used to assess wealth-related inequalities. Spatial analysis was done to identify the spatial distribution and multilevel logistic regression analysis was used to identify predictors of skilled birth attendance in Ethiopia. Analysis was done using STATA version 14, ArcGIS, and SaTscan software.

Results

The prevalence of skilled birth attendance was 50.04% (95% CI: 48.69%, 51.40%) in Ethiopia. Old age, being married, being educated, having television and radio, having ANC visits, being multiparous, having large household sizes, having a rich wealth index, living in rural residence, and living in a high level of community poverty and women's education were significant predictors of skilled birth attendance. Skilled birth attendance was disproportionately concentrated in rich households [C = 0.482; 95% CI: 0.436, 0.528]. High prevalence of unskilled birth attendance was found in Somalia, SNNP, Afar, and southern parts of the Amhara regions. Primary clusters of unskilled birth attendance Somalia and some parts of Oromia region of Ethiopia.

Conclusion

Half of the women in Ethiopia did not utilize skilled birth attendants with significant spatial clustering. Age, marital status, educational status, ANC Visit, having television and radio, parity, household size, wealth index, residence, community level poverty, and community level of women's education were significant predictors of skilled birth attendance. Skilled birth attendance was unevenly concentrated in rich households. The regions of Somalia, SNNP, Afar, and southern Amhara were identified as having a high prevalence of using unskilled birth attendance. Public health interventions should target those women at high risk of using unskilled birth attendants.
Rollercoasters and research: applying data-enabled design to a semi-public context
Authors: Raviselvam Sujithra , Lovei Peter , Noortman Renee , Olsson Thomas , Väänänen Kaisa , Funk Mathias
Published: August 2024

Introduction

Data-enabled design (DED) is a design approach where research data is integrated with design praxis in an iterative and hands-on fashion, and it has been successfully applied to design for people in the healthcare domain. However, how to utilise DED as a more broadly applicable approach that facilitates design for larger populations in semi-public spaces remains an open question. Understanding the advantages and challenges of applying DED in various contexts is crucial to guide design researchers further and prevent methodological mismatch.

Methods

In this article, we report on applying DED in an open, semi-public context with a large population of visitors. Here, DED was applied in projects with student design researchers who had limited control over the data gathered from people in the context.

Results

We share the results of a two-week design workshop that involved sixteen student design researchers and was organised in collaboration with Särkänniemi, one of Finland’s largest theme parks. Using design proposals and reflections from five teams, we investigate the use of DED in this context, reporting on perceived challenges, barriers, successes and future directions for taking the DED approach into similar (semi-)public contexts.

Discussions

We conclude with reflections from the workshop organisers’ point of view and share the feedback from our industry collaborator, highlighting challenges and opportunities in translating the DED approach to a new domain.
Exploring the ketogenic diet’s potential in reducing neuroinflammation and modulating immune responses
Authors: Monda Antonietta , La Torre Maria Ester , Messina Antonietta , Di Maio Girolamo , Monda Vincenzo , Moscatelli Fiorenzo , De Stefano Marida , La Marra Marco , Padova Marilena Di , Dipace Anna , Limone Pierpaolo , Casillo Maria , Monda Marcellino , Messina Giovanni , Polito Rita
Published: August 2024

The ketogenic diet (KD) is marked by a substantial decrease in carbohydrate intake and an elevated consumption of fats and proteins, leading to a metabolic state referred to as “ketosis,” where fats become the primary source of energy. Recent research has underscored the potential advantages of the KD in mitigating the risk of various illnesses, including type 2 diabetes, hyperlipidemia, heart disease, and cancer. The macronutrient distribution in the KD typically entails high lipid intake, moderate protein consumption, and low carbohydrate intake. Restricting carbohydrates to below 50 g/day induces a catabolic state, prompting metabolic alterations such as gluconeogenesis and ketogenesis. Ketogenesis diminishes fat and glucose accumulation as energy reserves, stimulating the production of fatty acids. Neurodegenerative diseases, encompassing Alzheimer’s disease, Parkinson’s disease are hallmarked by persistent neuroinflammation. Evolving evidence indicates that immune activation and neuroinflammation play a significant role in the pathogenesis of these diseases. The protective effects of the KD are linked to the generation of ketone bodies (KB), which play a pivotal role in this dietary protocol. Considering these findings, this narrative review seeks to delve into the potential effects of the KD in neuroinflammation by modulating the immune response. Grasping the immunomodulatory effects of the KD on the central nervous system could offer valuable insights into innovative therapeutic approaches for these incapacitating conditions.
Sphingosine-1-Phosphate Receptor 4 links neutrophils and early local inflammation to lymphocyte recruitment into the draining lymph node to facilitate robust germinal center formation
Authors: Luker Andrea J. , Wukitch Abigail , Kulinski Joseph M. , Ganesan Sundar , Kabat Juraj , Lack Justin , Frischmeyer-Guerrerio Pamela , Metcalfe Dean D. , Olivera Ana
Published: August 2024

The successful development of germinal centers (GC) relies heavily on innate mechanisms to amplify the initial inflammatory cascade. In addition to their role in antigen presentation, innate cells are essential for the redirection of circulating lymphocytes toward the draining lymph node (dLN) to maximize antigen surveillance. Sphingosine-1-Phosphate (S1P) and its receptors (S1PR1-5) affect various aspects of immunity; however, the role of S1PR4 in regulating an immune response is not well understood. Here we use a footpad model of localized TH1 inflammation to carefully monitor changes in leukocyte populations within the blood, the immunized tissue, and the dLN. Within hours of immunization, neutrophils failed to adequately mobilize and infiltrate into the footpad tissue of S1PR4-/- mice, thereby diminishing the local vascular changes thought to be necessary for redirecting circulating cells toward the inflamed region. Neutrophil depletion with anti-Ly6G antibodies significantly reduced early tissue edema as well as the redirection and initial accumulation of naïve lymphocytes in dLN of WT mice, while the effects were less prominent or absent in S1PR4-/- dLN. Adoptive transfer experiments further demonstrated that the lymphocyte homing deficiencies in vivo were not intrinsic to the donor S1PR4-/- lymphocytes, but were instead attributed to differences within the S1PR4-deficient host. Reduced cell recruitment in S1PR4-/- mice would seed the dLN with fewer antigen-respondent lymphocytes and indeed, dLN hypertrophy at the peak of the immune response was severely diminished, with attenuated GC and activation pathways in these mice. Histological examination of the S1PR4-/- dLN also revealed an underdeveloped vascular network with reduced expression of the leukocyte tethering ligand, PNAd, within high endothelial venule regions, suggesting inadequate growth of the dLN meant to support a robust GC response. Thus, our study reveals that S1PR4 may link early immune modulation by neutrophils to the initial recruitment of circulating lymphocytes and downstream expansion and maturation of the dLN, thereby contributing to optimal GC development during an adaptive response.
Minimal residual disease detection in lymphoma: methods, procedures and clinical significance
Authors: Zhang Sijun , Wang Xiangyu , Yang Zhenzhen , Ding Mengjie , Zhang Mingzhi , Young Ken H. , Zhang Xudong
Published: August 2024

Lymphoma is a highly heterogeneous lymphohematopoietic tumor. As our understanding of the biological and pathological characteristics of lymphoma improves, we are identifying an increasing number of lymphoma subtypes. Genotyping has enhanced our ability to diagnose, treat, and monitor the prognosis of lymphoma. Despite significant improvements in treatment effectiveness, traditional methods for assessing disease response and monitoring prognosis are imperfect, and there is no significant improvement in overall remission rates for lymphoma patients. Minimal Residual Disease (MRD) is often indicative of refractory disease or early relapse. For lymphoma patients, personalized MRD monitoring techniques offer an efficient means to estimate disease remission levels, predict early relapse risk, and assess the effectiveness of new drug regimens. In this review, we delve into the MRD procedures in lymphoma, including sample selection and requirements, detection methods and their limitations and advantages, result interpretation. Besides, we also introduce the clinical applications of MRD detection in lymphoma.
The microbial damage and host response framework: lesson learned from pathogenic survival trajectories and immunoinflammatory responses of Talaromyces marneffei infection
Authors: Pruksaphon Kritsada , Amsri Artid , Jeenkeawpieam Juthatip , Thammasit Patcharin , Nosanchuk Joshua D. , Youngchim Sirida
Published: August 2024

The adverse outcomes of fungal infection in mammalian hosts depend on the complex interactions between the host immune system and pathogen virulence-associated traits. The main clinical problems arise when the host response is either too weak to effectively eliminate the pathogen or overly aggressive, resulting in host tissue damage rather than protection. This article will highlight current knowledge regarding the virulence attributions and mechanisms involved in the dual-sided role of the host immune system in the immunopathogenesis of the thermally dimorphic fungus Talaromyces marneffei through the lens of the damage response framework (DRF) of microbial pathogenesis model.
Predicting antimicrobial properties of lignin derivatives through combined data driven and experimental approach
Authors: Kalinoski Ryan M. , Shao Qing , Shi Jian
Published: August 2024

Meta-analysis, experimental and data-driven quantitative structure–activity relationship (QSAR) models were developed to predict the antimicrobial properties of lignin derivatives. Five machine learning algorithms were applied to develop QSAR models based on the ChEMBL, a public non-lignin specific database. QSAR models were refined using ordinary-least-square regressions with a meta-analysis dataset extracted from literature and an experimental dataset. The minimum inhibition concentration (MIC) values of compounds in the meta-analysis dataset correlate to classification-based descriptors and the number of aliphatic carboxylic acid groups (R2 = 0.759). Comparatively, QSARs derived from the experimental datasets suggest that the number of aromatic hydroxyl groups were better predictors of Bacterial Load Difference (BLD, R2 = 0.831) for Bacillus subtilis, while the number of alkyl aryl groups were the strongest correlation in predicting the BLD (R2 = 0.682) of Escherichia coli. This study provides insights into the type of descriptors that correlate to antimicrobial activity and guides the valorization of lignin into sustainable antimicrobials for potential applications in food preservation, fermentation, and other industrial sectors.
Optical coherence tomography angiography findings in patients affected by giant cell arteritis, with and without ocular involvement: a pilot study
Authors: Vannozzi Lorenzo , Nicolosi Cristina , Vicini Giulio , Bacherini Daniela , Giattini Dario , Urban Maria Letizia , Palermo Adalgisa , Malandrino Danilo , Bello Federica , Virgili Gianni , Giansanti Fabrizio
Published: August 2024

Purpose

We evaluated the clinical features and retinal and disk perfusion characteristics by using optical coherence tomography (OCT) and optical coherence tomography angiography (OCTA) in a subset of giant cell arteritis (GCA) patients who manifested anterior ischemic optic neuropathy (AION), in a subset of GCA patients without ocular involvement, and in a control group composed of healthy controls.

Methods

We performed an observational study on the eyes of GCA patients affected by arteritic AION both in acute and chronic phases, unaffected eyes of AION, eyes of GCA patients without ocular involvement, and in a control group of healthy eyes of healthy individuals. All patients underwent a complete ophthalmic examination and an OCT and OCTA of the macula and the disk.

Results

The study evaluated 10 eyes of GCA patients with AION (AION group), 8 unaffected eyes of GCA patients with AION in another eye (unaffected eyes of AION group), 16 eyes of GCA patients without ocular involvement (non-ocular group), and 22 eyes of healthy patients (healthy group). The ganglion cell complex (GCC) superior and inferior thicknesses were significantly lower in the AION group compared to the unaffected eyes of the AION group (p = 0.045 and p = 0.034, respectively). All OCTA vascular density parameters of the optic disk analyzed in this study (optic nerve head (ONH) whole, superior, inferior, radial peripapillary capillary plexus (RPCP) whole, superior, inferior, lamina cribrosa (LC) whole, superior, inferior) resulted significantly lower in the AION group compared to the unaffected eyes group (p < 0.05 for all the comparisons). The ONH whole and inferior were statistically higher in the healthy group in comparison to the group of GCA patients without ocular involvement (p = 0.008 and p = 0.006, respectively). The ONH inferior was also statistically higher in the unaffected eyes of the AION group in comparison to the non-ocular group (p = 0.045). Regarding the OCTA macular vessel density parameters, the superficial capillary plexus (SCP), whole and inner, were statistically lower in the AION group compared with the unaffected eyes of the AION group.

Conclusion

We found a profound vascular impairment in eyes affected by AION and areas of hypoperfusion in the eyes of patients with GCA without ocular involvement, good BCVA, and no clinically significant features. We hypothesized that these areas of lower vessel density might represent areas of subclinical hypoperfusion that cannot be detected ophthalmoscopically.