bluuebunny/crossref_metadata_2025 huggingface dataset:
Institutional Responses to Drought in a High HIV Prevalence Setting in Rural South Africa
Authors: Orievulu Kingsley S. , Iwuji Collins C.
Published: January 2022

In 2015, South Africa experienced one of the worst (El Niño-induced) droughts in 35 years. This affected economic activities, individual and community livelihoods and wellbeing especially in rural communities in northern KwaZulu-Natal. Drought’s direct and indirect impacts on public health require urgent institutional responses, especially in South Africa’s stride to eliminate HIV as a public health threat by 2030 in line with the UNAIDS goals. This paper draws on qualitative data from interviews and policy documents to discuss how the devastating effect of the 2015 drought experience in the rural Hlabisa sub-district of uMkhanyakude, a high HIV prevalence area, imposes an imperative for more proactive institutional responses to drought and other climate-related events capable of derailing progress made in South Africa’s HIV/AIDS response. We found that drought had a negative impact on individual and community livelihoods and made it more difficult for people living with HIV to consistently engage with care due to economic losses from deaths of livestock, crop failure, food insecurity, time spent in search of appropriate water sources and forced relocations. It also affected government institutions and their interventions. Interviewed participants’ reflections on drought-related challenges, especially those related to institutional and coordination challenges, showed that although current policy frameworks are robust, their implementation has been stalled due to complex reporting systems, and inadequate interdepartmental collaboration and information sharing. We thus argue that to address the gaps in the institutional responses, there is a need for more inclusive systems of drought-relief implementation, in which government departments, especially at the provincial and district levels, work with national institutions to better share data/information about drought-risks in order to improve preparedness and implementation of effective mitigation measures.
Neurovascular Impairment and Therapeutic Strategies in Diabetic Retinopathy
Authors: Oshitari Toshiyuki
Published: January 2022

Diabetic retinopathy has recently been defined as a highly specific neurovascular complication of diabetes. The chronic progression of the impairment of the interdependence of neurovascular units (NVUs) is associated with the pathogenesis of diabetic retinopathy. The NVUs consist of neurons, glial cells, and vascular cells, and the interdependent relationships between these cells are disturbed under diabetic conditions. Clinicians should understand and update the current knowledge of the neurovascular impairments in diabetic retinopathy. Above all, neuronal cell death is an irreversible change, and it is directly related to vision loss in patients with diabetic retinopathy. Thus, neuroprotective and vasoprotective therapies for diabetic retinopathy must be established. Understanding the physiological and pathological interdependence of the NVUs is helpful in establishing neuroprotective and vasoprotective therapies for diabetic retinopathy. This review focuses on the pathogenesis of the neurovascular impairments and introduces possible neurovascular protective therapies for diabetic retinopathy.
Factors Associated with Knowledge, Attitudes, and Prevention towards HIV/AIDS among Adults 15–49 Years in Mizoram, North East India: A Cross-Sectional Study
Authors: Pachuau Lucy Ngaihbanglovi , Tannous Caterina , Agho Kingsley Emwinyore
Published: January 2022

Despite a campaign of effective educational interventions targeting knowledge, attitudes, and prevention, Human Immunodeficiency-Virus/Acquired Immune Deficiency Syndrome (HIV/AIDS) continues to be a significant public health issue in India, with Mizoram reporting the highest HIV/AIDS cases in 2018–2019. In this study, we extracted Mizoram state from the National Family Health Survey Fourth Series (NFHS-4) 2015–2016 datasets and investigated factors associated with respondents’ knowledge, attitudes, and prevention towards HIV/AIDS. The sample included 3555 adults aged 15–49 years residing in Mizoram, North-east India. Respondents who reported having ever heard of HIV/AIDS was 98%. Multivariate analysis indicated that the probability of having inadequate knowledge of HIV/AIDS was higher among those with no schooling, who were illiterate, of non-Christian faiths, belonging to backward tribes or caste, from poor households, and those who lived in rural areas, not exposed to media. The odds of mother-to-child transmission (PMTCT) of HIV/AIDS transmission was high among females (AOR = 3.12, 95% CI 2.34–4.16), respondents aged 35–39 years (AOR = 1.74, 95% CI 1.05–2.87) and those belonging to other backward class. The HIV/AIDS knowledge of respondents was found to be encouraging as the majority (98%) were considered to have a good level of understanding of the condition. An educational intervention to reduce the number of adults 15–49 years infected with HIV/AIDS in Mizoram should target those from low socioeconomic groups, those from non-Christian religions, and those from other backward classes.
Influence of Physical Activity and Sport on the Inclusion of People with Visual Impairment: A Systematic Review
Authors: Alcaraz-Rodríguez Virginia , Medina-Rebollo Daniel , Muñoz-Llerena Antonio , Fernández-Gavira Jesús
Published: January 2022

People with visual impairment have greater difficulty in accessing physical activity and sport, and a lack of social interaction is also associated with a risk of exclusion. Work is currently being done to include people with visual impairment through physical activity and sport. However, there is a lack of studies examining the status and overall effectiveness of interventions in the pre- and post-COVID stage. This study aimed to provide solid evidence on the characteristics and effectiveness of interventions for the inclusion of people with visual impairment through physical activity and sport in order to address the need for dissemination on this topic. The bibliographic search was carried out with the words “Physical activity”, “Physical exercise”, “Sport”, “Physical training”, “visual disability”, “visual impairment” and “inclusion” in the databases PubMed, Scopus, Web of Science and Google Scholar from 2018 to 2021.The article selection process was according to the PRISMA protocol with a final selection of nine articles. The main results highlighted that the programmes improve the perception of people with disabilities, increase social skills and health and increase the social importance of people with disabilities in the social environment. Among the most generalised conclusions were the need for specialised training, the need for social inclusion and participation of people with visual impairment in their environments and increased physical activity.
The Inter-Rater and Intra-Rater Reliability of the Actual Aquatic Skills Test (AAST) for Assessing Young Children’s Motor Competence in the Water
Authors: Mertens Lisa , De Martelaer Kristine , Sääkslahti Arja , D’Hondt Eva
Published: January 2022

As children’s actual aquatic skills are important for the prevention of drowning as well as their engagement in lifelong aquatic physical activity, researchers and practitioners should be able to assess this vital concept accurately and reliably. Therefore, this study aimed to investigate the inter-rater and intra-rater reliability of the Actual Aquatic Skills Test (AAST), consisting of 17 different test items for the assessment of young children’s motor competence in the water. Six raters received a training and evaluation session on scoring the AAST, after which five of them assessed four test videos (of various children (n = 38) performing the test items) twice, with one to two weeks in between (i.e., test and re-test). Inter-rater and intra-rater reliability were determined per test video and for the different AAST test items across videos using Gwet’s Agreement Coefficient 2 (Gwet’s AC2). The Gwet’s AC2 for inter-rater reliability at the test varied from 0.414 to 1.000, indicating a moderate to perfect agreement between raters. For intra-rater reliability, it ranged from 0.628 to 1.000, demonstrating a good to perfect agreement between test and re-test scoring. In conclusion, the AAST is a promising tool to reliably assess young children’s actual aquatic skills in an indoor swimming pool.
Diabetic Mastopathy. Review of Diagnostic Methods and Therapeutic Options
Authors: Guzik Paweł , Gęca Tomasz , Topolewski Paweł , Harpula Magdalena , Pirowski Wojciech , Koziełek Krzysztof , Żmuda Marcin , Śniadecki Marcin , Góra Tomasz , Basta Paweł , Czekierdowski Artur
Published: January 2022

Diabetic mastopathy is a rare breast condition that may occur in insulin-treated men and women of any age. The etiology is still unclear; however, the autoimmunological background of the disease is highly suspected. The changes in diabetic mastopathy may mimic breast cancer; therefore, its diagnostic process is demanding, and treatment options are not clear and limited. Lesions in DM are usually multiple; therefore, surgical removal is not fully effective. A well-done anamnesis with core-needle biopsy is essential and definitive in most cases. In this review, we summarize up-to-date knowledge of diagnostic methods and therapeutic options for diabetic mastopathy treatment and present three cases of diabetic mastopathy-type lesions in ultrasound and radiological examinations.
Planning for Implementation Success of an Electronic Cross-Facility Health Record for Pediatric Palliative Care Using the Consolidated Framework for Implementation Research (CFIR)
Authors: Busse Theresa Sophie , Kernebeck Sven , Dreier Larissa Alice , Meyer Dorothee , Zenz Daniel , Haas Peter , Zernikow Boris , Ehlers Jan Peter
Published: January 2022

Pediatric palliative care (PPC) patients require years of care across professions and sectors. Sharing treatment-related information and communicating among different PPC professionals is critical to ensure good quality of care. In Germany, this communication is mostly paper-based and prone to errors. Therefore, an electronic cross-facility health record (ECHR) was participatorily designed with users, wherein information can be shared and PPC professionals can communicate with each other. As this form of electronic health record differs from existing models in Germany, there is a need for successful implementation to ensure a positive impact. Therefore, the facilitators and barriers to the implementation of ECHR in PPC were examined. Using the consolidated framework for implementation research (CFIR), transcripts of 32 interviews, 3 focus groups, and 20 think-aloud studies with PPC professionals were analyzed. CFIR indicated that the ECHR-design was viewed positively by users and can be a facilitator for implementation. Barriers exist, mainly due to the fact that the implementation is not planned, the use of the ECHR involves effort, costs are not covered, and all users must be motivated to use the ECHR for functionality. CFIR helps uncover the crux of the issues that need to be considered when planning ECHR implementation to improve care in PPC.
Postactivation Performance Enhancement (PAPE) Increases Vertical Jump in Elite Female Volleyball Players
Authors: Villalon-Gasch Lamberto , Penichet-Tomas Alfonso , Sebastia-Amat Sergio , Pueo Basilio , Jimenez-Olmedo Jose M.
Published: January 2022

The purpose of this study was to verify if a conditioning activity was effective to elicit postactivation performance enhancement (PAPE) and to increase the performance in vertical jump (VJ) in elite female volleyball players. Eleven national Superliga-2 volleyball players (22.6 ± 3.5 years) were randomly assigned to an experimental and control group. Countermovement jumps (CMJ) were performed on eight occasions: before (Pre-PAPE) and after activation (Post-PAPE), after the match (Pre-Match), and after each of the five-match sets (Set 1 to 5). ANOVA showed significantly increased jump performance for the experiment between baseline (Pre-PAPE) and all the following tests: +1.3 cm (Post-PAPE), +3.0 cm (Pre-Match), +4.8 cm (Set 1), +7.3 cm (Set 2), +5.1 cm (Set 3), +3.6 cm (Set 4), and +4.0 cm (Set 5), all showing medium to large effect size (0.7 < ES < 2.4). The performance of the control group did not show significant increases until Set 3 (+3.2 cm) and Set 5 (+2.9 cm), although jump heights were always lower for the control group than the experimental. The use of conditioning activity generates increased VJ performance in Post-PAPE tests and elicited larger PAPE effects that remain until the second set of a volleyball match.
Additive and Interactive Associations of Environmental and Sociodemographic Factors with the Genotypes of Three Glutathione S-Transferase Genes in Relation to the Blood Arsenic Concentrations of Children in Jamaica
Authors: Rahbar Mohammad H. , Samms-Vaughan Maureen , Zhao Yuansong , Saroukhani Sepideh , Zaman Sheikh F. , Bressler Jan , Hessabi Manouchehr , Grove Megan L. , Shakspeare-Pellington Sydonnie , Loveland Katherine A.
Published: January 2022

Arsenic (As) is a metalloid that has been classified as a xenobiotic with toxic effects on human beings, especially on children. Since the soil in Jamaica contains As, dietary intake is considered the main source of As exposure in Jamaicans. In addition, glutathione S-transferase (GST) genes, including GSTT1, GSTP1, and GSTM1, play an important role in the metabolism of xenobiotics including As in humans. Using data from 375 typically developing children (2–8 years) in Jamaica, we investigated the environmental and sociodemographic factors, as well as their possible interactions with the children’s genotype for GST genes in relation to having a detectable level of blood As concentration (i.e., >1.3 μg/L). Using multivariable logistic regression, we have identified environmental factors significantly associated with blood As concentrations that include a child’s age, parental education levels, and the consumption of saltwater fish, cabbage, broad beans, and avocado (all p < 0.01). Based on the multivariable analysis including gene x environment interactions, we found that among children with the Ile/Ile genotype for GSTP1 Ile105Val, children who consumed avocado had higher odds of having a detectable blood As concentration compared to children who did not eat avocado.
Training-Associated Superior Visuomotor Integration Performance in Elite Badminton Players after Adjusting for Cardiovascular Fitness
Authors: Chen Yi-Liang , Hsu Jen-Hao , Tai Dana Hsia-Ling , Yao Zai-Fu
Published: January 2022

Badminton is recognized as the fastest racket sport in the world based on the speed of the birdie which can travel up to 426 km per hour. On the badminton court, players are not only required to track the moving badminton birdie (visual tracking and information integration) but also must anticipate the exact timing to hit it back (temporal estimation). However, the association of training experience related to visuomotor integration or temporal prediction ability remains unclear. In this study, we tested this hypothesis by examining the association between training experience and visuomotor performances after adjusting for age, education, and cardiovascular fitness levels. Twenty-eight professional badminton players were asked to perform a compensatory tracking task and a time/movement estimation task for measuring visuomotor integration and temporal prediction, respectively. Correlation analysis revealed a strong association between training experience and performance on visuomotor integration, indicating badminton training may be promoted to develop visuomotor integration ability. Furthermore, the regression model suggests training experience explains 32% of visuomotor integration performances. These behavioral findings suggest badminton training may facilitate the perceptual–cognitive performance related to visuomotor integration. Our findings highlight the potential training in visuomotor integration may apply to eye–hand coordination performance in badminton sport.
Can Tourism Development Make Cities More Livable? Investigating 40 Cities in China
Authors: Kang Lei , Yang Zhaoping , Dang Yunxiao , Zhang Wenzhong , Liu Caicai
Published: January 2022

The field of rapid urbanization has recently paid more attention to the relationship between tourism development and liveable city construction. Previous studies have mainly focused on the experiences of tourists in tourist cities and seldom paid attention to the perceptions of local residents. Based on survey data of nearly 10,000 permanent residents in 40 key tourist cities in China, this study uses a multilevel model to quantitatively analyse the natural environment characteristics, sociocultural environment characteristics and comprehensive attraction of tourism in different tourist cities to explore their impact on urban liveability satisfaction. Results show that the developed tourist cities do not exactly correspond to the cities with a high liveability evaluation. The objective evaluation of both the natural environment and the sociocultural environment has an important influence on the liveability of cities, but the influence of the natural environment is stronger than that of the sociocultural environment. An intermediary effect exists in the subjective evaluation of the natural environment and environments for liveability perception. Simultaneously, residents’ liveability satisfaction varies according to their age, education level, annual household income and other social and economic conditions. These findings provide insights for developing countries to further improve residents’ living quality and urban construction under the condition of the rapid development of tourism.
Lower-Limb Muscle Contractile Properties, Explosive Power and the Subjective Response of Elite Soccer Players to the COVID-19 Lockdown
Authors: Paravlic Armin H. , Simunic Bostjan , Pisot Sasa , Kleva Matej , Teraz Kaja , Vogrin Matjaz , Marusic Uros , Pisot Rado
Published: January 2022

The present study examined the effects of the lockdown period on basic anthropometric measures, countermovement jumping performance, skeletal muscle contractile properties derived from tensiomyography (TMG), injury incidence, and self-assessed general well-being in elite soccer players. A total of 266 players were assessed before (PRE) and 32 players were reassessed 11 days after (POST) the COVID-19 period. Significant changes in the TMG parameters were observed POST compared to PRE: contraction time (Tc) increased from 6% to 50% in vastus lateralis [VL] (p = 0.009) and biceps femoris [BF] (p < 0.001), respectively; whereas radial displacement (Dm) increased for 19% in BF (p = 0.036) and 17% in VL (p < 0.001), respectively. Jumping performance remained unchanged from PRE to POST In addition, athletes rated the lockdown period as a positive event and felt psychologically better during the lockdown, primarily because they spent more time with family members and friends. Although there were no differences in any of the variables describing lower limb muscle power following the two-month lockdown, the altered contractile properties of the assessed muscles suggest suboptimal conditioning of the football players.
The Economic Burden and Determinant Factors of Parents/Caregivers of Children with Cerebral Palsy in Malaysia: A Mixed Methods Study
Authors: Ismail Aniza , Sk Abd Razak Ruhana , Suddin Leny Suzana , Mahmud Aidalina , Kamaralzaman Sazlina , Yusri Ghazali
Published: January 2022

The economic burden is a major concern for parents/caregivers of children with cerebral palsy (CP). This study used the sequential explanatory mixed-method approach to explorethe economic burden on parents/caregivers with a CP child in Malaysia and the factors associated with the economic burden. The study period spanned April 2020 and December 2020. A total of 106 questionnaire respondents were selected for the quantitative part, and 15 were interviewed to obtain qualitative input. A retrospective costing analysis was conducted based on the cost data obtained from the questionnaire. The majority of the children were GrossMotor Function Classification System (GMFCS) Level 5 (71%), quadriplegic (63%), and aged >4 years (90%). The estimated annual median total economic burden on the parents/caregivers per child in 2020 was RM52,540.00 (~USD12,515.03), with indirect cost being the greatest cost (RM28,800.00, ~USD6860.16), followed by developmental cost (RM16,200.00, ~USD3858.84), direct healthcare cost (RM4540.00, ~USD1081.43) and direct non-healthcare cost (RM3000.00, ~USD714.60). The annual household income was identified as a significant determinant factor (p=0.019, 95% CI: 0.04, 0.40) of the economic burden. The participants’ responses during the in-depth interview in the qualitative part of the study supported the premise that socioeconomic factors play a substantial role in determining the total economic burden. Our findings may aid local policymakers when planning the greater provision of support to the affected families in the future, especially for the parents/caregivers of children with CP, who are facing socioeconomic challenges.
A Retrospective Cohort Study of the Association between Serum Osteopontin Levels and Aortic Stiffness in Hypertensive Patients
Authors: Chang Yuan-Chieh , Tsai Jen-Pi , Wang Ji-Hung , Hsu Bang-Gee
Published: January 2022

By suppressing mineralization and preventing ectopic calcium deposits, osteopontin (OPN) has an inhibitory effect on vascular calcification. Also, there is an association between OPN and aortic stiffness (AS). We aimed to investigate the association between serum OPN levels and AS measured by carotid–femoral pulse wave velocity (cfPWV) in hypertensive patients. Baseline characteristics and fasting blood sampling of 120 participants with hypertension and 120 participants without hypertension were acquired. Serum OPN concentrations were determined by enzyme-linked immunosorbent assay. In total, 43 (35.9%) participants were assigned to the AS group with cfPWV of >10 m/s in hypertensive patients. There were more patients with diabetes mellitus, old age, high systolic blood pressure, high serum intact parathyroid hormone (iPTH), elevated C-reactive protein, and high OPN levels in the AS group compared with the control group in hypertensive participants. A multivariate logistic regression analysis discloses that age, SBP, serum OPN, and iPTH levels were independently associated with AS in hypertensive patients. Moreover, according to a multivariate forward stepwise linear regression analysis, OPN level is positively associated with cfPWV. In conclusion, serum OPN level is assumed to be a potential biomarker to predict AS and is positively associated with cfPWV in patients with hypertension.
Source Apportionment and Geographic Distribution of Heavy Metals and as in Soils and Vegetables Using Kriging Interpolation and Positive Matrix Factorization Analysis
Authors: Su Huiyue , Hu Yueming , Wang Lu , Yu Huan , Li Bo , Liu Jiangchuan
Published: January 2022

Food security and cultivated land utilization can be seriously affected by heavy metal (HM) pollution of the soil. Therefore, identifying the pollution sources of farmland is the way to control soil pollution and enhance soil quality effectively. In this research, 95 surface soil samples, 34 vegetable samples, 27 irrigation water samples, and 20 fertilizer samples were collected from the Wuqing District of Tianjin City, China and was used to determine their HMs accumulation and potential ecological risks. Then, kriging interpolation and positive matrix factorization (PMF) were utilized to identify the sources of soil HMs. The results indicated that soil HMs in the study area were contaminated at a medium level, but that the pollution of Cd was more severe, and the Cd content in vegetables was slightly higher than the permissible threshold (0.02 mg·kg−1). Furthermore, a non-homogeneous distribution was observed, with higher concentrations of HM contaminants concentrated in the southwest of the study area, where many metal manufacturing industries are located. Our results suggest that the Cd originated from industrial activity; As and Pb from agricultural practices; Ni, Cu, Cr, and As mainly from natural sources; Zn and Cu from organic fertilizer; Pb and Cd mainly from traffic discharge; and Cr, Ni, and Pb from sewage irrigation. Obviously, the accumulation of soil HMs in the study area could be mainly attributed to industrial activities, implying the need for implementation of government strategies to reduce industrial point-source pollution.
Recovery of Regular Daily Physical Activities Prevents Residual Dizziness after Canalith Repositioning Procedures
Authors: Martellucci Salvatore , Stolfa Andrea , Castellucci Andrea , Pagliuca Giulio , Clemenzi Veronica , Terenzi Valentina , Malara Pasquale , Attanasio Giuseppe , Gazia Francesco , Gallo Andrea
Published: January 2022

Objective: Residual dizziness is a disorder of unknown pathophysiology, which may occur after repositioning procedures for benign paroxysmal positional vertigo. This study evaluates the relationship between regular daily physical activity and the development of residual dizziness after treatment for benign paroxysmal positional vertigo. Study Design: Prospective observational cohort study. Setting: Academic university hospital. Methods: Seventy-one patients admitted with benign paroxysmal positional vertigo involving the posterior semicircular canal were managed with Epley’s procedure. Three days after successful treatment, the patients underwent a telephone interview to investigate vertigo relapse. If the patients no longer complained of vertigo, they were asked about symptoms consistent with residual dizziness. Subsequently, they were asked about the recovery of physical activities they regularly performed prior to the onset of vertigo. Results: Sixty-nine patients (age: 57.79 ± 15.05) were enrolled: five (7.24%) reported vertigo relapse whereas twenty-one of sixty-four non-relapsed patients (32.81%) reported residual dizziness. A significant difference in the incidence of residual dizziness was observed considering the patients’ age (p = 0.0003). Of the non-relapsed patients, 46 (71.88%) recovered their regular dynamic daily activities after treatment and 9 (19.57%) reported residual dizziness, while 12 of the 18 patients (66.67%) who did not resume daily activity reported residual symptoms (p = 0.0003). A logistic regression analysis showed a significant association between daily activity resumption and lack of residual dizziness (OR: 14.01, 95% CI limits 3.14–62.47; p = 0.001). Conclusions: Regardless of age, the resumption of regular daily physical activities is associated with a lack of residual dizziness.
Spatial Differentiation and Influencing Factors of Water Pollution-Intensive Industries in the Yellow River Basin, China
Authors: Du Haibo , Ji Xuepeng , Chuai Xiaowei
Published: January 2022

The structure adjustment and layout optimization of water pollution-intensive industries (WPIIs) are crucial to the health and sustainable development of the watershed life community. Based on micro-detailed data of Chinese industrial enterprises from 2003 to 2013, we analyzed and revealed the spatial differentiation characteristics and influencing factors of WPIIs in the Yellow River Basin (YRB) from 2003 to 2013 by constructing a water pollution-intensive index and integrating kernel density estimation and geographically weighted regression models from a watershed perspective. The results show that: (1) the scale of WPIIs in the YRB showed a growth trend from 2003 to 2013, and the output value increased from 442.5 billion yuan in 2003 to 6192.4 billion yuan in 2013, an increase of 13 times. (2) WPIIs are generally distributed in an east-west direction, and their spatial distribution is river-side, with intensive distribution in the downstream areas and important tributaries such as Fen River and Wei River. (3) WPIIs are generally clustered in high density downstream, but the spatial clustering characteristics of different industries varied significantly. The chemical industries, paper industries, etc. were mainly concentrated in downstream areas. Processing of food from agricultural products was distributed in the upper, middle and downstream areas. Resource-intensive industries such as coal and oil were concentrated in energy-rich midstream areas. (4) Natural resource endowment was the main factor affecting the distribution of WPIIs in the midstream and upstream areas of the basin, and technological innovation played a significant role in the distribution of downstream industries. The level of economic development and industrial historical foundation promoted the geographical concentration of industries. The scale of wastewater discharge and the proximity of rivers influenced the concentration of industries in the midstream and downstream.
Official Statistics, Building Censuses, and OpenStreetMap Completeness in Italy
Authors: Salvucci Gianluigi , Salvati Luca
Published: January 2022

The present study provides a simplified framework verifying the degree of coverage and completeness of settlement maps derived from the OpenStreetMap (OSM) database at the national scale, with a possible use in official statistics. Measuring the completeness of the objects (i.e., buildings) derived from OpenStreetMap database supports its potential use in building/population censuses and other diachronic surveys, as well as administrative sources such as the register of building permits and land-use cadasters. A series of measurements at different scales are proposed and tested for Italy, in line with earlier studies. While recognizing the potential of the OpenStreetMap database for official statistics, the present work underlines the urgent need of an additional (spatially explicit) analysis overcoming the data heterogeneity and sub-optimal coverage of the OSM information source.
YB-1 Phosphorylation at Serine 209 Inhibits Its Nuclear Translocation
Authors: Sogorina Ekaterina M. , Kim Ekaterina R. , Sorokin Alexey V. , Lyabin Dmitry N. , Ovchinnikov Lev P. , Mordovkina Daria A. , Eliseeva Irina A.
Published: January 2022

YB-1 is a multifunctional DNA- and RNA-binding protein involved in cell proliferation, differentiation, and migration. YB-1 is a predominantly cytoplasmic protein that is transported to the nucleus in certain conditions, including DNA-damaging stress, transcription inhibition, and viral infection. In tumors, YB-1 nuclear localization correlates with high aggressiveness, multidrug resistance, and a poor prognosis. It is known that posttranslational modifications can regulate the nuclear translocation of YB-1. In particular, well-studied phosphorylation at serine 102 (S102) activates YB-1 nuclear import. Here, we report that Akt kinase phosphorylates YB-1 in vitro at serine 209 (S209), which is located in the vicinity of the YB-1 nuclear localization signal. Using phosphomimetic substitutions, we showed that S209 phosphorylation inhibits YB-1 nuclear translocation and prevents p-S102-mediated YB-1 nuclear import.
Butein and Frondoside-A Combination Exhibits Additive Anti-Cancer Effects on Tumor Cell Viability, Colony Growth, and Invasion and Synergism on Endothelial Cell Migration
Authors: Sulaiman Shahrazad , Arafat Kholoud , Al-Azawi Aya Mudhafar , AlMarzooqi Noura Abdulraouf , Lootah Shamsa Nasser Ali Hussain , Attoub Samir
Published: January 2022

Despite the significant advances in targeted- and immuno-therapies, lung and breast cancer are at the top list of cancer incidence and mortality worldwide as of 2020. Combination therapy consisting of a mixture of different drugs taken at once is currently the main approach in cancer management. Natural compounds are extensively investigated for their promising anti-cancer potential. This study explored the anti-cancer potential of butein, a biologically active flavonoid, on two major solid tumors, namely, A549 lung and MDA-MB-231 breast cancer cells alone and in combination with another natural anti-cancer compound, frondoside-A. We demonstrated that butein decreases A549 and MDA-MB-231 cancer cell viability and colony growth in vitro in addition to tumor growth on chick embryo chorioallantoic membrane (CAM) in vivo without inducing any noticeable toxicity. Additionally, non-toxic concentrations of butein significantly reduced the migration and invasion of both cell lines, suggesting its potential anti-metastatic effect. We showed that butein anti-cancer effects are due, at least in part, to a potent inhibition of STAT3 phosphorylation, leading to PARP cleavage and consequently cell death. Moreover, we demonstrated that combining butein with frondoside-A leads to additive effects on inhibiting A549 and MDA-MB-231 cellular viability, induction of caspase 3/7 activity, inhibition of colony growth, and inhibition of cellular migration and invasion. This combination reached a synergistic effect on the inhibition of HUVECs migration in vitro. Collectively, this study provides sufficient rationale to further carry out animal studies to confirm the relevance of these compounds’ combination in cancer therapy.
Biocompatibility and Electrical Stimulation of Skeletal and Smooth Muscle Cells Cultured on Piezoelectric Nanogenerators
Authors: Blanquer Andreu , Careta Oriol , Anido-Varela Laura , Aranda Aida , Ibáñez Elena , Esteve Jaume , Nogués Carme , Murillo Gonzalo
Published: January 2022

Nanogenerators are interesting for biomedical applications, with a great potential for electrical stimulation of excitable cells. Piezoelectric ZnO nanosheets present unique properties for tissue engineering. In this study, nanogenerator arrays based on ZnO nanosheets are fabricated on transparent coverslips to analyse the biocompatibility and the electromechanical interaction with two types of muscle cells, smooth and skeletal. Both cell types adhere, proliferate and differentiate on the ZnO nanogenerators. Interestingly, the amount of Zn ions released over time from the nanogenerators does not interfere with cell viability and does not trigger the associated inflammatory response, which is not triggered by the nanogenerators themselves either. The local electric field generated by the electromechanical nanogenerator–cell interaction stimulates smooth muscle cells by increasing cytosolic calcium ions, whereas no stimulation effect is observed on skeletal muscle cells. The random orientation of the ZnO nanogenerators, avoiding an overall action potential aligned along the muscle fibre, is hypothesised to be the cause of the cell-type dependent response. This demonstrates the need of optimizing the nanogenerator morphology, orientation and distribution according to the potential biomedical use. Thus, this study demonstrates the cell-scale stimulation triggered by biocompatible piezoelectric nanogenerators without using an external source on smooth muscle cells, although it remarks the cell type-dependent response.
Anti-Inflammatory, Antioxidant, Moisturizing, and Antimelanogenesis Effects of Quercetin 3-O-β-D-Glucuronide in Human Keratinocytes and Melanoma Cells via Activation of NF-κB and AP-1 Pathways
Authors: Ha Anh Thu , Rahmawati Laily , You Long , Hossain Mohammad Amjad , Kim Jong-Hoon , Cho Jae Youl
Published: January 2022

Quercetin 3-O-β-D-glucuronide (Q-3-G), the glucuronide conjugate of quercetin, has been reported as having anti-inflammatory properties in the lipopolysaccharide-stimulated macrophages, as well as anticancer and antioxidant properties. Unlike quercetin, which has been extensively described to possess a wide range of pharmacological activities including skin protective effects, the pharmacological benefits and mechanisms Q-3-G in the skin remained to be elucidated. This study focused on characterizing the skin protective properties, including anti-inflammatory and antioxidant properties, of Q-3-G against UVB-induced or H2O2-induced oxidative stress, the hydration effects, and antimelanogenesis activities using human keratinocytes (HaCaT) and melanoma (B16F10) cells. Q-3-G down-regulated the expression of the pro-inflammatory gene and cytokine such as cyclooxygenase-2 (COX-2) and tumor necrosis factor (TNF)-α in H2O2 or UVB-irradiated HaCaT cells. We also showed that Q-3-G exhibits an antioxidant effect using free radical scavenging assays, flow cytometry, and an increased expression of nuclear factor erythroid 2- related factor 2 (Nrf2). Q-3-G reduced melanin production in α-melanocyte-stimulating hormone (α-MSH)-induced B16F10 cells. The hydration effects and mechanisms of Q-3-G were examined by evaluating the moisturizing factor-related genes, such as transglutaminase-1 (TGM-1), filaggrin (FLG), and hyaluronic acid synthase (HAS)-1. In addition, Q-3-G increased the phosphorylation of c-Jun, Jun N-terminal kinase (JNK), Mitogen-activated protein kinase (MAPK) kinase 4 (MKK4), and TAK1, involved in the MAPKs/AP-1 pathway, and the phosphorylation of IκBα, IκB kinase (IKK)-α, Akt, and Src, involved in the NF-κB pathway. Taken together, we have demonstrated that Q-3-G exerts anti-inflammatory, antioxidant, moisturizing, and antimelanogenesis properties in human keratinocytes and melanoma cells through NF-κB and AP-1 pathways.
Multi-Omics Profiling in Marfan Syndrome: Further Insights into the Molecular Mechanisms Involved in Aortic Disease
Authors: Verhagen Judith M. A. , Burger Joyce , Bekkers Jos A. , den Dekker Alexander T. , von der Thüsen Jan H. , Zajec Marina , Brüggenwirth Hennie T. , van der Sterre Marianne L. T. , van den Born Myrthe , Luider Theo M. , van IJcken Wilfred F. J. , Wessels Marja W. , Essers Jeroen , Roos-Hesselink Jolien W. , van der Pluijm Ingrid , van de Laar Ingrid M. B. H. , Brosens Erwin
Published: January 2022

Thoracic aortic aneurysm is a potentially life-threatening disease with a strong genetic contribution. Despite identification of multiple genes involved in aneurysm formation, little is known about the specific underlying mechanisms that drive the pathological changes in the aortic wall. The aim of our study was to unravel the molecular mechanisms underlying aneurysm formation in Marfan syndrome (MFS). We collected aortic wall samples from FBN1 variant-positive MFS patients (n = 6) and healthy donor hearts (n = 5). Messenger RNA (mRNA) expression levels were measured by RNA sequencing and compared between MFS patients and controls, and between haploinsufficient (HI) and dominant negative (DN) FBN1 variants. Immunohistochemical staining, proteomics and cellular respiration experiments were used to confirm our findings. FBN1 mRNA expression levels were highly variable in MFS patients and did not significantly differ from controls. Moreover, we did not identify a distinctive TGF-β gene expression signature in MFS patients. On the contrary, differential gene and protein expression analysis, as well as vascular smooth muscle cell respiration measurements, pointed toward inflammation and mitochondrial dysfunction. Our findings confirm that inflammatory and mitochondrial pathways play important roles in the pathophysiological processes underlying MFS-related aortic disease, providing new therapeutic options.
Novel Purine Derivative ITH15004 Facilitates Exocytosis through a Mitochondrial Calcium-Mediated Mechanism
Authors: de Pascual Ricardo , Calzaferri Francesco , Gonzalo Paula C. , Serrano-Nieto Rubén , de los Ríos Cristóbal , García Antonio G. , Gandía Luis
Published: January 2022

Upon depolarization of chromaffin cells (CCs), a prompt release of catecholamines occurs. This event is triggered by a subplasmalemmal high-Ca2+ microdomain (HCMD) generated by Ca2+ entry through nearby voltage-activated calcium channels. HCMD is efficiently cleared by local mitochondria that avidly take up Ca2+ through their uniporter (MICU), then released back to the cytosol through mitochondrial Na+/Ca2+ exchanger (MNCX). We found that newly synthesized derivative ITH15004 facilitated the release of catecholamines triggered from high K+-depolarized bovine CCs. Such effect seemed to be due to regulation of mitochondrial Ca2+ circulation because: (i) FCCP-potentiated secretory responses decay was prevented by ITH15004; (ii) combination of FCCP and ITH15004 exerted additive secretion potentiation; (iii) such additive potentiation was dissipated by the MICU blocker ruthenium red (RR) or the MNCX blocker CGP37157 (CGP); (iv) combination of FCCP and ITH15004 produced both additive augmentation of cytosolic Ca2+ concentrations ([Ca2+]c) K+-challenged BCCs, and (v) non-inactivated [Ca2+]c transient when exposed to RR or CGP. On pharmacological grounds, data suggest that ITH15004 facilitates exocytosis by acting on mitochondria-controlled Ca2+ handling during K+ depolarization. These observations clearly show that ITH15004 is a novel pharmacological tool to study the role of mitochondria in the regulation of the bioenergetics and exocytosis in excitable cells.
Development of Tools for Synergy of Social Functions of the State and Housing Mortgage Loans in Russia: Regional Analysis of the Central, Southern and Volga Federal Districts
Authors: Semenyuta Olga , Dubinina Irina , Degtyarev Anton
Published: January 2022

The article researches the features of the synergy of the social functions of the state and the housing mortgage loan (HML) in order to develop a tool that allows determining guidelines and directions for strengthening the effectiveness of collaboration between the state and the private sector represented by commercial banks in solving the most important social problem—providing housing to the population. The authors show that the use of the proposed assessment tool by state structures and commercial banks increases the effectiveness of solutions to the housing problem in the country and enhances the synergetic effect of a comprehensive increase in the standard of living of the population when synchronizing actions. The main purpose of the research was to develop an algorithm that determines the key factors influencing the number of issued HML. The object of the study is the Russian HML market on the example of three federal districts. The developed algorithm is based on the use of statistical analysis methods ANOVA, mutual regression and recursive feature elimination. The approbation of the results obtained on three subjects of the Russian Federation allowed us to obtain a set of significant factors of influence, taking into account regional peculiarities.
The Time-Varying Relation between Stock Returns and Monetary Variables
Authors: McMillan David G.
Published: January 2022

The nature of the relation between stock returns and the three monetary variables of interest rates (bond yields), inflation and money supply growth, while oft studied, is one that remains unclear. We argue that the nature of the relation changes over time, and this variation is largely driven by shocks, with a change in risk associated with each variable shifting the pattern of behaviour. We show a change in the correlation between each of the three variables with stock returns. Notably, a predominantly negative correlation with bond yields and inflation becomes positive, while the opposite is true for money supply growth. The shift begins with the bursting of the dotcom bubble but is exacerbated by the financial crisis. Results of predictive regressions for stock returns also indicate a switch in behaviour. Predominantly negative predictive power switches temporarily to positive around economic shocks. This suggests that higher yields, inflation and money growth typically depress returns but support the market during periods of stress. However, after the financial crisis, higher inflation and money growth exhibit persistent positive predictive power and suggest a change in the risk perception of higher values.
Vocal Learning and Behaviors in Birds and Human Bilinguals: Parallels, Divergences and Directions for Research
Authors: Sakata Jon T. , Birdsong David
Published: January 2022

Comparisons between the communication systems of humans and animals are instrumental in contextualizing speech and language into an evolutionary and biological framework and for illuminating mechanisms of human communication. As a complement to previous work that compares developmental vocal learning and use among humans and songbirds, in this article we highlight phenomena associated with vocal learning subsequent to the development of primary vocalizations (i.e., the primary language (L1) in humans and the primary song (S1) in songbirds). By framing avian “second-song” (S2) learning and use within the human second-language (L2) context, we lay the groundwork for a scientifically-rich dialogue between disciplines. We begin by summarizing basic birdsong research, focusing on how songs are learned and on constraints on learning. We then consider commonalities in vocal learning across humans and birds, in particular the timing and neural mechanisms of learning, variability of input, and variability of outcomes. For S2 and L2 learning outcomes, we address the respective roles of age, entrenchment, and social interactions. We proceed to orient current and future birdsong inquiry around foundational features of human bilingualism: L1 effects on the L2, L1 attrition, and L1<–>L2 switching. Throughout, we highlight characteristics that are shared across species as well as the need for caution in interpreting birdsong research. Thus, from multiple instructive perspectives, our interdisciplinary dialogue sheds light on biological and experiential principles of L2 acquisition that are informed by birdsong research, and leverages well-studied characteristics of bilingualism in order to clarify, contextualize, and further explore S2 learning and use in songbirds.
Acrylate and Methacrylate Polymers’ Applications: Second Life with Inexpensive and Sustainable Recycling Approaches
Authors: Corsaro Carmelo , Neri Giulia , Santoro Antonio , Fazio Enza
Published: January 2022

Polymers are widely employed in several fields thanks to their wide versatility and the easy derivatization routes. However, a wide range of commercial polymers suffer from limited use on a large scale due to their inert nature. Nowadays, acrylate and methacrylate polymers, which are respectively derivatives of acrylic or methacrylic acid, are among the most proposed materials for their useful characteristics like good biocompatibility, capping ability toward metal clusters, low price, potentially recyclability and reusability. Here, we discuss the advantages and challenges of this class of smart polymers focusing our attention on their current technological applications in medical, electronic, food packaging and environmental remediation fields. Furthermore, we deal with the main issue of their recyclability, considering that the current commercial bioplastics are not yet able to meet the global needs as much as to totally replace fossil-fuel-based products. Finally, the most accredited strategies to reach recyclable composites based on acrylic polymers are described.