bluuebunny/crossref_metadata_2025 huggingface dataset:
Differences in Temporal Relapse Characteristics Between Affective and Non-affective Psychotic Disorders: Longitudinal Analysis
Authors: Immanuel Sarah A. , Schrader Geoff , Bidargaddi Niranjan
Published: February 2021

Objective: Multiple relapses over time are common in both affective and non-affective psychotic disorders. Characterizing the temporal nature of these relapses may be crucial to understanding the underlying neurobiology of relapse.

Materials and Methods: Anonymized records of patients with affective and non-affective psychotic disorders were collected from SA Mental Health Data Universe and retrospectively analyzed. To characterize the temporal characteristic of their relapses, a relapse trend score was computed using a symbolic series-based approach. A higher score suggests that relapse follows a trend and a lower score suggests relapses are random. Regression models were built to investigate if this score was significantly different between affective and non-affective psychotic disorders.

Results: Logistic regression models showed a significant group difference in relapse trend score between the patient groups. For example, in patients who were hospitalized six or more times, relapse score in affective disorders were 2.6 times higher than non-affective psychotic disorders [OR 2.6, 95% CI (1.8–3.7), p < 0.001].

Discussion: The results imply that the odds of a patient with affective disorder exhibiting a predictable trend in time to relapse were much higher than a patient with recurrent non-affective psychotic disorder. In other words, within recurrent non-affective psychosis group, time to relapse is random.

Conclusion: This study is an initial attempt to develop a longitudinal trajectory-based approach to investigate relapse trend differences in mental health patients. Further investigations using this approach may reflect differences in underlying biological processes between illnesses.
Inventory and Analysis of Controlled Trials of Mobile Phone Applications Targeting Substance Use Disorders: A Systematic Review
Authors: Bahadoor Rubaab , Alexandre Jean-Marc , Fournet Lucie , Gellé Thibaut , Serre Fuschia , Auriacombe Marc
Published: February 2021

Background: Less than 20% of people with addictions have access to adequate treatment. Mobile health could improve access to care. No systematic review evaluates effectiveness of mobile health applications for addiction.

Objectives: First aim was to describe controlled trials evaluating the effectiveness of smartphone applications targeting substance use disorders and addictive behaviors. Secondly, we aimed to understand how the application produced changes in behavior and craving management.

Method: A systematic review based on PRISMA recommendations was conducted on MEDLINE, CENTRAL, and PsycINFO. Studies had to be controlled trials concerning addictive disorders (substance/behavior), mobile application-based interventions, assessing effectiveness or impact of those applications upon use, published after 2008. Relevant information was systematically screened for synthesis. Quality and risk of bias were evaluated with JADAD score.

Results: Search strategy retrieved 22 articles (2014-2019) corresponding to 22 applications targeting tobacco, alcohol, other substances and binge eating disorder. Control groups had access to usual treatments or a placebo-application or no treatment. Eight applications showed reduced use. Most of the applications informed about risks of use and suggestions for monitoring use. Twelve applications managed craving.

Discussion: Heterogeneity limited study comparisons. Duration of studies was too short to predict sustainable results. A reduction of craving seemed related to a reduction in use.

Conclusion: There is a lack of robust and comparable studies on mHealth applications for addiction treatment. Such applications could become significant contributors in clinical practice in the future so longer-termed double-blind studies are needed. Targeting craving to prevent relapse should be systematic.
Parents' Experiences of Receiving Professional Support Through Extended Home Visits During Pregnancy and Early Childhood—A Phenomenographic Study
Authors: Bäckström Caroline , Thorstensson Stina , Pihlblad Jessica , Forsman Anna-Carin , Larsson Margaretha
Published: February 2021

Background: While becoming a parent can be challenging for all, it can particularly be challenging for those parents and children who are in a vulnerable situation—e.g., in families whose members have problems related to health, relationships, or socioeconomic status. It is essential for health care professionals to identify the more vulnerable families at an early stage. Home visits are one cost-effective way of identifying and supporting such families. This study describes the parental experiences of an intervention that involves professional support in the form of extended home visits. The aim of the study is to describe the parents' understanding of their experiences of receiving professional support through extended home visits both during pregnancy and the first 15 months of their child's life.

Methods/Design: A phenomenographic approach was used. Semi-structured interviews were conducted with 12 parents who had received the intervention. The interviews were analyzed using the seven-step phenomenography model described by Sjöström and Dahlgren.

Results: The following three descriptive categories emerged from the analysis: (1) conceptions concerning the meaning of the physical environment, (2) conceptions concerning extended home visits promoting feelings of self-confidence in the parental role, and (3) conceptions concerning extended home visits promoting parental participation and relations.

Conclusion and Clinical Implications: Extended home visits as a form of professional support appear to promote parental self-confidence in parenting ability, giving parents a feeling of security that facilitates conversation with professionals. Children and their entire families had natural roles during home visits, which allowed the children to behave more characteristically. Furthermore, the home visits were understood to facilitate social support through social activities at the child health center as well as integration into Swedish society for migrant parents. Professional support should be adjusted to the unique individual needs of parents, which demands a variety of supportive interventions—for example, reorganizing one or two of the regular clinical visits currently being scheduled as home visits instead.
Intravesical Recurrence After Radical Nephroureterectomy of Upper Urinary Tract Urothelial Carcinoma: A Large Population-Based Investigation of Clinicopathologic Characteristics and Survival Outcomes
Authors: Wu Jie , Xu Pei-Hang , Luo Wen-Jie , Dai Bo , Shen Yi-Jun , Ye Ding-Wei , Wang Yu-Chen , Zhu Yi-Ping
Published: February 2021

Background: Of patients with upper urinary tract urothelial carcinoma (UTUC), 22–47% developed bladder recurrence after radical nephroureterectomy. Furthermore, the effect of surgery for UTUC-bladder cancer (BC) has not been well validated. The aim of this study was to assess the impact of standard primary BC surgical strategy on survival of patients diagnosed with UTUC-BC.

Patients and Methods: A total of 676 UTUC-BC patients and 197,753 primary BC patients diagnosed from 2004 to 2016, were identified based on the SEER database. The Kaplan-Meier method and the Fine and Gray competing risks analysis were performed to assess overall survival (OS) and cancer-specific mortality (CSM). Multivariate Cox regression model and competing risks regression model were used to identify independent risk factors. Propensity score matching (PSM) was also performed to adjust potential confounding factors.

Results: The baseline characteristics and survival outcomes of the two BC patient cohorts are quite different. For UTUC-BC patients, no significant difference in OS (NMIBC: p = 0.88; MIBC: p = 0.98) or cumulative incidence of CSM (NMIBC: p = 0.12; MIBC: p = 0.96) were noted for various surgical procedures. Local tumor treatment and partial cystectomy for UTUC-NMIBC patients produced lower 1-year (6.1%) and 3-year CSM (16.2%). Radical cystectomy for UTUC-MIBC patients produced lower 1-year (11.8%) but higher 3-year CSM (62.7%). After PSM for covariates, UTUC-BC patients still had a worse prognosis after surgery compared with primary BC patients. Based on regression models, older age, advanced T stage, N positive disease, M positive disease, and shorter interval between UTUC and BC were identified as independent risk factors for UTUC-BC patients.

Conclusion: Standard primary BC surgical strategy did not provide significant survival benefit for UTUC-BC patients. Compared with primary BC patients, UTUC-BC patients had a worse prognosis after surgery, suggesting that current primary BC surgical guidelines are not entirely appropriate for UTUC-BC patients. Our findings underscore the continued importance and need for better prognosis and improved guidelines for management of UTUC-BC patients.
Extended Robotic Pulmonary Resections
Authors: Scheinerman Joshua A. , Jiang Jeffrey , Chang Stephanie H. , Geraci Travis C. , Cerfolio Robert J.
Published: February 2021

While lung cancer remains the most common cause of cancer-related mortality in the United States, surgery for curative intent continues to be a mainstay of therapy. The robotic platform for pulmonary resection for non-small cell lung cancer (NSCLC) has been utilized for more than a decade now. With respect to more localized resections, such as wedge resection or lobectomy, considerable data exist demonstrating shorter length of stay, decreased postoperative pain, improved lymph node dissection, and overall lower complication rate. There are a multitude of technical advantages the robotic approach offers, such as improved optics, natural movement of the operator's hands to control the instruments, and precise identification of tissue planes leading to a more ergonomic and safe dissection. Due to the advantages, the scope of robotic resections is expanding. In this review, we will look at the existing data on extended robotic pulmonary resections, specifically post-induction therapy resection, sleeve lobectomy, and pneumonectomy. Additionally, this review will examine the indications for these more complex resections, as well as review the data and outcomes from other institutions' experience with performing them. Lastly, we will share the strategy and outlook of our own institution with respect to these three types of extended pulmonary resections. Though some controversy remains regarding the use and safety of robotic surgery in these complex pulmonary resections, we hope to shed some light on the existing evidence and evaluate the efficacy and safety for patients with NSCLC.
Detection of Representative Variables in Complex Systems with Interpretable Rules Using Core-Clusters
Authors: Champion Camille , Brunet Anne-Claire , Burcelin Rémy , Loubes Jean-Michel , Risser Laurent
Published: February 2021

In this paper, we present a new framework dedicated to the robust detection of representative variables in high dimensional spaces with a potentially limited number of observations. Representative variables are selected by using an original regularization strategy: they are the center of specific variable clusters, denoted CORE-clusters, which respect fully interpretable constraints. Each CORE-cluster indeed contains more than a predefined amount of variables and each pair of its variables has a coherent behavior in the observed data. The key advantage of our regularization strategy is therefore that it only requires to tune two intuitive parameters: the minimal dimension of the CORE-clusters and the minimum level of similarity which gathers their variables. Interpreting the role played by a selected representative variable is additionally obvious as it has a similar observed behaviour as a controlled number of other variables. After introducing and justifying this variable selection formalism, we propose two algorithmic strategies to detect the CORE-clusters, one of them scaling particularly well to high-dimensional data. Results obtained on synthetic as well as real data are finally presented.
Study and Improvement of Acoustic Conditions in Public Spaces of Shopping Malls
Authors: Kanev Nikolay
Published: February 2021

Acoustic comfort in shopping malls is one of the aspects that influence the shopping environment. Making enjoyable and comfortable retail and public spaces is demanded when designing and managing properties. This paper focuses on the study of the public spaces with a large volume in the operating malls, which are perceived as acoustically uncomfortable. The main reason for poor acoustic conditions is a too long reverberation, as the measured reverberation time reached 4–5 s at middle frequencies. The usual way to improve the acoustics is to apply sound-absorbing materials on space surfaces. Two examples of improving acoustic conditions in food courts are given. In both cases, the reverberation time was reduced by about half, and changes in acoustics were considered significant and positive. It is shown the Sabine’s equation is valid for considered spaces. Average absorption coefficients are found by means of the Sabine’s equation and can be used for quick estimation of the reverberation time in similar spaces without the absorbing treatment. Based on the analysis of this and other studies, a method for qualitative assessment of the public space acoustics is proposed.
Samsung vs. Apple: How Different Communication Strategies Affect Consumers in Portugal
Authors: Almeida Margarida , Sousa Eva , Rodrigues Carolina , Candeias Maria Beatriz , Au-Yong-Oliveira Manuel
Published: February 2021

It is indisputable that technology is present in everyday life. In this digital era, brands need to adapt to the changing pace of the needs and desires of society to distinguish themselves from their adversaries, especially in the electronic environment. Hence, they must have well-defined and successful marketing and advertising strategies to achieve a place on the podium of preference of consumers. This work intends to understand how the communication strategies of Apple and Samsung affect the decisions of consumers in Portugal to buy electronic devices. To this end, a survey was conducted, and the responses of 700 individuals who reside in Portugal were analysed through descriptive and inferential (chi-square test of independence) statistics. The survey results show that cost-benefit, price, technical specifications, and performance are the characteristics that weigh the most when purchasing electronic devices, as well as the perceived prestige of the brand. Additionally, an association was found between having only one device and having more products of the brand, with Apple users having more frequently more than one of the brand’s products than Samsung users. We thus concluded that Apple consumers are more loyal. It was also found that the store where the devices are brought is not independent, in Portugal, of the brand of the devices. Apple users buy more brand products from the brand store, both physical and online, than Samsung users. Finally, advertisements and word-of-mouth were found to be fundamental for consumers to acknowledge brand devices, and the degree in which this happens is also not independent of the brand, in Portugal, as a chi-square independence test showed.
An Ultrasonic-Based Detection of Air-Leakage for the Unclosed Components of Aircraft
Authors: Lyu Yanlin , Jamil Muhammad , Ma Pengfei , He Ning , Gupta Munish Kumar , Khan Aqib Mashood , Pimenov Danil Yurievich
Published: February 2021

Air-leakage detection is among the most important processes at the assembly stage of unclosed components, especially for large aircraft. A series of air-leakage detecting methods are generally applied during the final assembly, nevertheless, many of them are less effective to detect the leakage at the assembly stage. The present study aims to discuss the principles of ultrasonic generation in negative pressure conditions to detect the air-leakage. An ultrasonic-based detection method is proposed and designed to detect the air-leakage of unclosed components for aircraft. A relationship between the acoustic power, sound pressure, and the leak aperture detection distance was identified and discussed. A leakage rate model related to leakage rate, leak aperture, and system pressure was implemented and confirmed through experiments. Findings have indicated that the air-leakage can be detected effectively within a detection distance of 0.8 m and a leak aperture greater or equal to 0.4 mm with this method. Besides, the leak location, leak aperture, and leakage rate was acquired in an accurate and fast way. It is an effective method of detecting the air-leakage of unclosed components at the aircraft assembly stage reducing the testing time, energy consumption, and cost for the air-leakage detection in the final assembly stage of large aircraft.
Spectral Correlation for Signal Presence Detection and Frequency Acquisition of Small Satellites
Authors: Hofmann Jonas , Knopp Andreas , Spooner Chad M. , Minelli Giovanni , Newman James
Published: February 2021

Challenges in interference-limited satellite detection arising from the low-earth orbit (LEO) and the Industrial, Scientific and Medical (ISM) frequency bands are addressed. In particular, a novel signal presence detector based on cyclostationary signal properties is proposed and analyzed for a low signal-to-noise-plus-interference ratio (SINR) regime. The performance of the proposed detector, which is applicable to various small-satellite scenarios, is evaluated on both simulated and real-world measurement data. This measurement data has been collected from the scientific satellite mission “Picosats Realizing Orbital Propagation Calibrations using Beacon Emitters” (PROPCUBE).
Methane Emission and Milk Production from Jersey Cows Grazing Perennial Ryegrass–White Clover and Multispecies Forage Mixtures
Authors: Loza Cecilia , Reinsch Thorsten , Loges Ralf , Taube Friedhelm , Gere José Ignacio , Kluß Christof , Hasler Mario , Malisch Carsten S.
Published: February 2021

Methane is a major constituent of greenhouse gas (GHG) emissions from ruminants, and mitigation strategies are needed to alleviate this negative environmental impact while maintaining the environmental and other benefits of grazing systems. Forages containing plant-specialized metabolites (PSM), particularly condensed tannins, may help reduce enteric methane (CH4) emissions. However, information on in vivo CH4 emissions from cows grazing mixtures that contain bioactive herbs is scarce. Accordingly, this study compared a binary mixture of perennial ryegrass (Lolium perenne) and white clover (Trifolium repens) against a diverse mixture of six additional species, including tannin-rich species like birdsfoot trefoil (Lotus corniculatus) and salad burnet (Sanguisorba minor), in a full-grazing dairy system. Enteric CH4 emissions were measured using the SF6 tracer technique. Cows grazing diverse mixtures increased their energy-corrected milk (ECM) yield by 4% (p < 0.001) compared with binary mixtures. However, CH4 emissions per kg ECM were also 11% greater for the diverse mixtures (p < 0.05). The very high feed quality and milk yield from both mixtures explained the low CH4 emissions recorded relative to the milk output. The addition of forbs did not provide additional benefits at these intensities, as they were maintained in low yield shares throughout.
Remote-Controlled Monitoring of Flying Pests with an Electrostatic Insect Capturing Apparatus Carried by an Unmanned Aerial Vehicle
Authors: Kakutani Koji , Matsuda Yoshinori , Nonomura Teruo , Takikawa Yoshihiro , Osamura Kazumi , Toyoda Hideyoshi
Published: February 2021

The purpose of the study was to construct an electrostatic insect-capturing apparatus that could be applied to a drone (quadcopter). For this purpose, a double-charged dipolar electric field screen (DD-screen) was constructed using oppositely charged insulator tubes that was then attached to a drone. For charging, the inner surface of the tubes was coated with a conductive paste and then linked to a negative or positive voltage generator. The opposite charges of the tubes formed an electric field between them and created an attractive force to capture insects that entered the field. The DD-screen constructed here was sufficiently light to enable its attachment to a drone. The screen was hung from the drone perpendicular to the direction of drone movement, so as to receive the longitudinal airflow produced by the movement of the drone. It was positioned 1.8 m below the drone body to avoid the influence of the downward slipstream generated by the rotating propellers. Eventually, the drone was able to conduct a stable flight, with sufficient endurance, and captured airborne insects carried by an airflow of 8 m/s during the flight. This study, therefore, provides an experimental basis for establishing a new method for conducting trap-based monitoring of airborne insects during remote-controlled flight through operation of a DD-screen attached to a drone.
Micronutrients and Soil Microorganisms in the Suppression of Potato Common Scab
Authors: Kopecky Jan , Rapoport Daria , Sarikhani Ensyeh , Stovicek Adam , Patrmanova Tereza , Sagova-Mareckova Marketa
Published: February 2021

Nature-friendly approaches for crop protection are sought after in the effort to reduce the use of agrochemicals. However, the transfer of scientific findings to agriculture practice is relatively slow because research results are sometimes contradictory or do not clearly lead to applicable approaches. Common scab of potatoes is a disease affecting potatoes worldwide, for which no definite treatment is available. That is due to many complex interactions affecting its incidence and severity. The review aims to determine options for the control of the disease using additions of micronutrients and modification of microbial communities. We propose three approaches for the improvement by (1) supplying soils with limiting nutrients, (2) supporting microbial communities with high mineral solubilization capabilities or (3) applying communities antagonistic to the pathogen. The procedures for the disease control may include fertilization with micronutrients and appropriate organic matter or inoculation with beneficial strains selected according to local environmental conditions. Further research is proposed to use metagenomics/metabolomics to identify key soil–plant–microbe interactions in comparisons of disease-suppressive and -conducive soils.
Crop Establishment and Weed Control Options for Sustaining Dry Direct Seeded Rice Production in Eastern India
Authors: Saha Sanjoy , Munda Sushmita , Singh Sudhanshu , Kumar Virender , Jangde Hemant Kumar , Mahapatra Ashirbachan , Chauhan Bhagirath S.
Published: February 2021

Dry direct seeded rice (DSR) has emerged as an economically viable alternative to puddled transplanted rice to address emerging constraints of labor and water scarcity and the rising cost of cultivation. However, wide adoption of DSR is seriously constrained by weed management trade-off. Therefore, the availability of effective weed control options is critical for the success and wide-scale adoption of DSR. A field study was conducted at ICAR-National Rice Research Institute, Cuttack, India, in the dry seasons of 2015 and 2016 to evaluate the performance of three crop establishment methods and five weed control practices on weed management, productivity, profitability and energetics of dry DSR. The results demonstrated that weed density and weed dry weight was lower in drill seeding than broadcast seeding by 26–36% and manual line-seeding by 16–24%, respectively, at 30 and 60 days after crop emergence (DAE). Among herbicides, post-emergence application (17 DAE) of azimsulfuron was most effective in controlling weeds compared to early post application of bispyribac-sodium and bensulfuron-methyl+pretilachlor. Weed competition in the weedy check treatment resulted 58% reduction in rice yield. Among establishment methods, drill-seeding was most profitable with US $ 685 ha−1 higher net income than broadcast seeding primarily due to higher yield. Among weed control treatments, azimsulfuron was most profitable resulting in US $ 160 and 736 ha−1 higher net income than weed free and weedy check, respectively. The specific energy was lowest for drill seeding among establishment method and azimsulfuron among weed control practices, suggesting lowest energy consumed in producing per unit of grain yield.
Zinc Uptake by Plants as Affected by Fertilization with Zn Sulfate, Phosphorus Availability, and Soil Properties
Authors: Recena Ramiro , García-López Ana M. , Delgado Antonio
Published: February 2021

Zinc (Zn) deficiency constrains crop yield and quality, but soil factors influencing Zn availability to plants and reactions of applied Zn fertilizer are not fully understood. This work is aimed at studying Zn availability in soil and the use efficiency of Zn fertilizers by plants as affected by soil properties and particularly by soil available P. We performed a pot experiment involving four consecutive crops fertilized with Zn sulfate using 36 soils. The cumulative Zn uptake and dry matter yield in the four crops increased with increased initial diethylenetriamine pentaacetic acid extraction of Zn (DTPA-Zn) (R2 = 0.75 and R2 = 0.61; p < 0.001). The initial DTPA-Zn increased with increased Olsen P (R2 = 0.41; p < 0.001) and with increased ratio of Fe in poorly crystalline to Fe in crystalline oxides (R2 = 0.58; p < 0.001). DTPA-Zn decreased with increased cumulative Zn uptake, but not in soils with DTPA-Zn < 0.5 mg kg−1. Overall, the available Zn is more relevant in explaining Zn uptake by plants than applied Zn sulfate. However, in Zn-deficient soils, Zn fertilizer explained most of the Zn uptake by crops. Poorly crystalline Fe oxides and P availability exerted a positive role on Zn availability to plants in soil.
Effects of Premating Calcium and Phosphorus Supplementation on Reproduction Efficiency of Grazing Yak Heifers
Authors: Zhou Jia , Zhang Jianxun , Xue Benchu , Yue Shuangming , Yang Chao , Xue Bai
Published: February 2021

Reproductive efficiency is the main factor limiting yak production on the Tibet Plateau. The purpose of this study was to investigate the effect of supplementation with calcium chloride (CaCl) and monocalcium phosphate (MCP) for 30 days before breeding on body weight (BW) change, serum bone metabolism biomarkers, conception rate, and calving rate of grazing yaks. Ninety 3 year old yak heifers (153.05 ± 6.56 kg BW) were assigned to three treatments (n = 30 per treatment): grazing without supplementation (CONT), grazing plus calcium chloride supplementation (CaCl), and grazing plus monocalcium phosphate supplementation (MCP). Compared with the CONT group, supplementation with CaCl increased the serum concentrations of osteocalcin and decreased the alkaline phosphatase (ALP) levels (p < 0.05); supplementation with MCP increased the average daily gain (ADG), serum concentrations of phosphorus (P) and osteocalcin, conception rate, and calving rate (p < 0.05), whereas it decreased the serum concentrations of hydroxyproline, ALP, and calcitonin (p < 0.05). Both CaCl and MCP supplementation had no effect on serum calcium (Ca) concentration. The ADG, conception rate, and calving rate were higher in the MCP group than in the CaCl group (p < 0.05), while the serum concentrations of hydroxyproline and calcitonin were lower (p < 0.05). It could be concluded that premating supplementation with MCP increased the body weight gain and subsequent conception and calving rate of grazing yaks. Supplementation with MCP had a positive effect on body condition and bone metabolism, thus providing a better estrous condition for grazing yak heifers, which could contribute to enhancing reproduction efficiency.
Effect of a Phytogenic Water Additive on Growth Performance, Blood Metabolites and Gene Expression of Amino Acid Transporters in Nursery Pigs Fed with Low-Protein/High-Carbohydrate Diets
Authors: Shili Cedrick N. , Habibi Mohammad , Sutton Julia , Barnes Jessie , Burch-Konda Jacob , Pezeshki Adel
Published: February 2021

The objective of this study was to investigate the effect of a phytogenic water additive (PWA) on growth performance and underlying factors involved in pigs fed with low-protein (LP)/high-carbohydrate diets. Forty-eight weaned barrows were allotted to six treatments for 4 weeks: CON-NS, control (CON) diet-no PWA; CON-LS, CON diet-low dose PWA (4 mL/L); CON-HS, CON diet-high dose PWA (8 mL/L); LP-NS, LP diet-no PWA; LP-LS, LP diet-low dose PWA; LP-HS, LP diet-high dose PWA. Relative to CON-NS, pigs fed with CON-HS had increased average daily gain, body weight and serum calcium (Ca) and phosphorous (P) and had decreased mRNA abundance of solute carrier family 7 member 11 and solute carrier family 6 member 19 in jejunum. Compared to LP-NS, pigs fed with LP-HS had increased muscle lean%, decreased muscle fat%, decreased serum Ca and increased serum P. Compared to their NS counterparts, CON-LS, CON-HS, and LP-LS increased the concentration of plasma essential AA and those fed with CON-HS and LP-HS tended to reduce the abundance of the solute carrier family 7 member 1 transcript in skeletal muscle. Thus, PWA improved the performance of weaned pigs fed with protein-adequate diets likely through increased blood essential AA and affected the muscle composition when dietary protein was deficient.
European Consumers’ Willingness to Pay for Red Meat Labelling Attributes
Authors: Cubero Dudinskaya Emilia , Naspetti Simona , Arsenos Georgios , Caramelle-Holtz Emmanuelle , Latvala Terhi , Martin-Collado Daniel , Orsini Stefano , Ozturk Emel , Zanoli Raffaele
Published: February 2021

Food consumption in Europe is changing. Red meat consumption has been steadily decreasing in the past decades. The rising interest of consumers for healthier and more sustainable meat products provides red meat producers with the opportunity to differentiate their offers by ecolabels, origin and health claims. This international study analyses the European consumer preferences for red meat (beef, lamb and goat) in seven countries: Finland, France, Greece, Italy, Spain, Turkey and the United Kingdom. Through a choice experiment, 2900 responses were collected. Mixed multinomial logit models were estimated to identify heterogeneous preferences among consumers at the country level. The results indicate substantial differences between the most relevant attributes for the average consumer, as well as their willingness to pay for them in each country. Nevertheless, national origin and organic labels were highly valued in most countries.
Effects of the Monomeric Components of Poly-hydroxybutyrate-co-hydroxyhexanoate on the Growth of Vibrio penaeicida In Vitro and on the Survival of Infected Kuruma Shrimp (Marsupenaeus japonicus)
Authors: Fukami Kimio , Takagi Fumika , Sonoda Kohei , Okamoto Hiroshi , Kaneno Daisuke , Horikawa Takao , Takita Masaki
Published: February 2021

Here, we investigated the inhibitory effects of the biodegradable, water-insoluble polymer poly-hydroxybutyrate-co-hydroxyhexanoate (PHBH) and its two constituent monomers, the hydroxyalkanoic acids 3-hydroxybutyrate (3HB) and 3-hydroxyhexanoate (3HH), on the growth of the shrimp-pathogenic bacterium Vibrio penaeicida. In vitro experiments revealed that 3HH showed greater growth inhibitory activity than 3HB against V. penaeicida. In addition, the activities of hydroxyalkanoic acids were pH dependent, being greater at pH 6.0 than at pH 7.0. Investigation of the pH of the shrimp gut revealed a pH range of 5.9–6.7 (mean 6.29 ± SD 0.20), indicating that the physiological environment was suitable for 3HB and 3HH to exert their inhibitory activities against V. penaeicida. In vivo bacterial challenge experiments revealed that survival rates in kuruma shrimp (Marsupenaeus japonicus) infected by V. penaeicida were significantly increased in shrimp reared on feed containing PHBH (0.1%–5% w/w PHBH) compared with that in shrimp reared on standard diet alone. Supplementation with PHBH had no significant effects on three shrimp growth parameters: increase in body weight, daily feeding rate, and feed conversion ratio. These results suggest that supplementation of standard diet with PHBH will increase shrimp resistance to infection by V. penaeicida, thereby increasing shrimp aquaculture productivity.
Using Decision Science for Monitoring Threatened Western Snowy Plovers to Inform Recovery
Authors: Marcot Bruce G. , Lyons James E. , Elbert Daniel C. , Todd Laura
Published: February 2021

Western Snowy Plovers (Charadrius nivosus nivosus) are federally listed under the US Endangered Species Act as Threatened. They occur along the US Pacific coastline and are threatened by habitat loss and destruction and excessive levels of predation and human disturbance. Populations have been monitored since the 1970s for distribution, reproduction, and survival. Since the species was federally listed in 1993 and a recovery plan was approved under the US Fish and Wildlife Service in 2007, recovery actions have resulted in growing populations with increased presence at breeding and wintering sites throughout their Pacific Coast range. This success has created logistical challenges related to monitoring a recovering species and a need for identifying and instituting the best monitoring approach given recovery goals, budgets, and the likelihood of monitoring success. We devised and implemented a structured decision analysis to evaluate nine alternative monitoring strategies. The analysis included inviting plover biologists involved in monitoring to score each strategy according to a suite of performance measures. Using multi-attribute utility theory, we combined scores across the performance measures for each monitoring strategy, and applied weighted utility values to show the implications of tradeoffs and find optimal decisions. We evaluated four scenarios for weighting the monitoring objectives and how risk attitude affects optimal decisions. This resulted in identifying six strategies that best meet recovery needs and were Pareto optimal for cost-effective monitoring. Results were presented to the US Fish and Wildlife Service, responsible for monitoring as well as for consideration to ensure consistent monitoring methods across the species’ range. Our use of structured decision-making can be applied to cases of other species once imperiled but now on the road to recovery.
Role of Antimicrobial Susceptibility Testing before First-Line Treatment Containing Clarithromycin for Helicobacter pylori Eradication in the Clinical Setting
Authors: Kang Seokin , Kim Yuri , Ahn Ji Yong , Jung Hwoon-Yong , Kim Nayoung , Na Hee Kyong , Lee Jeong Hoon , Jung Kee Wook , Kim Do Hoon , Choi Kee Don , Song Ho June , Lee Gin Hyug
Published: February 2021

Background: Checking Helicobacter pylori susceptibility tests in the clinical setting before first-line treatment is considered difficult. We compared susceptibility-guided therapy (SGT) with empirical therapy (ET) as a first-line treatment containing clarithromycin and investigated the eradication rate using antimicrobial susceptibility testing (AST). Methods: 257 patients with H. pylori infection, with AST, performed before the eradication of clarithromycin-containing regimens were enrolled and divided into two groups: the SGT and ET groups. Results: Eradication rates in the SGT and ET groups were 85.4% and 58.4% (P < 0.01), respectively. In triple therapy (TT), eradication rates of the SGT and ET groups were 85.1% and 56.6% (P < 0.01), respectively. In sequential therapy (SET), eradication rates of the SGT and ET groups were 86.2% and 65.6% (P = 0.06), respectively. According to AST, TT had an eradication rate of 84.6% with strains susceptible to clarithromycin and amoxicillin and 11.1% with strains resistant to both. SET had an eradication rate of 89.5% with strains susceptible to clarithromycin, amoxicillin, and metronidazole, whereas it was 0% with strains resistant to clarithromycin and metronidazole. Conclusions: SGT as first-line treatment improved eradication rates of TT and SET by 28.5 (P < 0.01) and 20.6 (P = 0.06) percent points, respectively, compared with ET.
Knowledge, Attitude, and Practices (KAP) Survey among Veterinarians, and Risk Factors Relating to Antimicrobial Use and Treatment Failure in Dairy Herds of India
Authors: Vijay Deepthi , Bedi Jasbir Singh , Dhaka Pankaj , Singh Randhir , Singh Jaswinder , Arora Anil Kumar , Gill Jatinder Paul Singh
Published: February 2021

The indiscriminate usage of antimicrobials in the animal health sector contributes immensely to antimicrobial resistance (AMR). The present study aims to assess the antimicrobial usage pattern and risk factors for AMR in animal husbandry sector of India. A cross-sectional survey about Knowledge, Attitude, and Practices (KAP) among veterinarians was carried out using a questionnaire comprising of 52 parameters associated with antibiotic use and the emergence of AMR in dairy herds. Respondents’ KAP scores were estimated to rank their level of knowledge, attitude, and practice. Furthermore, risk factors associated with treatment failure were analyzed by univariable and multivariable analyses. Out of a total of 466 respondents, the majority had average knowledge (69.5%), neutral attitude (93.2%), and moderate practice (51.3%) scores toward judicious antibiotic usage. Veterinarians reported mastitis (88.0%), reproductive disorders (76.6%), and hemoprotozoan infections (49.6%) as the top three disease conditions that require antibiotic usage. Most of the veterinarians (90.6%) resorted to their “own experience” as the main criteria for antibiotic choice. The use of the highest priority critically important antimicrobials (HPCIA) listed by the World Health Organization (WHO) in animals, particularly quinolones (76.8%) and third-generation cephalosporins (47.8%), has been reported. On multivariable regression analysis of the risk factors, the lack of cooperation of the dairy farmers in the completion of a prescribed antibiotic course by the veterinarian and the demand for antibiotic use even in conditions not requiring antibiotic use were found to be significantly associated with the outcome variable “treatment failure” having respective odds of 1.8 (95%CI: 1.1–3.0) and 3.6 (95%CI: 2.3–5.8) (p < 0.05). The average KAP score of veterinarians, poor farm management practices, lack of awareness among farmers on prudent antibiotic use, and lack of antibiotic stewardship are the significant factors that need attention to combat the rising AMR in veterinary sector in India.
Spread of Linezolid-Resistant Enterococcus spp. in Human Clinical Isolates in the Czech Republic
Authors: Mališová Lucia , Jakubů Vladislav , Pomorská Katarína , Musílek Martin , Žemličková Helena
Published: February 2021

The aim of this study was to map and investigate linezolid resistance mechanisms in linezolid-resistant enterococci in the Czech Republic from 2009 to 2019. Altogether, 1442 isolates of Enterococcus faecium and Enterococcus faecalis were examined in the National Reference Laboratory for Antibiotics. Among them, 8% of isolates (n = 115) were resistant to linezolid (E. faecium/n = 106, E. faecalis/n = 9). Only three strains of E. faecium were resistant to tigecycline, 72.6% of isolates were resistant to vancomycin. One isolate of E. faecium harbored the cfr gene. The majority (87%, n = 11) of E. faecium strains were resistant to linezolid because of the mutation G2576T in the domain V of the 23S rRNA. This mutation was detected also in two strains of E. faecalis. The presence of the optrA gene was the dominant mechanism of linezolid resistance in E. faecalis isolates. None of enterococci contained cfrB, poxtA genes, or any amino acid mutation in genes encoding ribosomal proteins. No mechanism of resistance was identified in 4 out of 106 E. faecium linezolid resistant isolates in this study. Seventeen sequence types (STs) including four novel STs were identified in this work. Clonal complex CC17 was found in all E. faecium isolates.
Mitochondrial Oxidative and Nitrosative Stress as a Therapeutic Target in Diseases
Authors: Marí Montserrat , Colell Anna
Published: February 2021

Mitochondria are fundamental to life [...]
Health Benefits of Antioxidative Peptides Derived from Legume Proteins with a High Amino Acid Score
Authors: Matemu Athanasia , Nakamura Soichiro , Katayama Shigeru
Published: February 2021

Legumes such as soybean, chickpea, lentil, cowpea, and mung bean, are valuable sources of protein with a high amino acid score and can provide bioactive peptides. This manuscript presents a review on legume-derived peptides, focusing on in vitro and in vivo studies on the potential antioxidative activities of protein hydrolysates and their characterization, amino acid sequences, or purified/novel peptides. The health implications of legume-derived antioxidative peptides in reducing the risks of cancer and cardiovascular diseases are linked with their potent action against oxidation and inflammation. The molecular weight profiles and amino acid sequences of purified and characterized legume-derived antioxidant peptides are not well established. Therefore, further exploration of legume protein hydrolysates is necessary for assessing the potential applications of antioxidant-derived peptides in the functional food industry.
Health Potential of Aloe vera against Oxidative Stress Induced Corneal Damage: An “In Vitro” Study
Authors: Ceravolo Ida , Mannino Federica , Irrera Natasha , Squadrito Francesco , Altavilla Domenica , Ceravolo Giorgia , Pallio Giovanni , Minutoli Letteria
Published: February 2021

Fuchs endothelial corneal dystrophy (FECD) is characterized by the gradual deterioration of corneal endothelial cells (CECs) and is the most common cause of corneal transplantation worldwide. CECs apoptosis caused by oxidative stress plays a pivotal role in the pathogenesis of FECD. Antioxidant compounds have been of considerable significance as a candidate treatment in the management of corneal diseases. Based on these findings, the objective of this study was to evaluate the effects of an aloe extract with antioxidant properties, in an “in vitro” model of FECD. Human corneal epithelial (HCE) cells were preincubated with aloe extract 100 μg/mL, two hours before hydrogen peroxide (H2O2) stimulus. H2O2 challenge significantly reduced the cell viability, increased the generation of Reactive Oxygen Species (ROS) and malondialdehyde levels. Moreover, m-RNA expression and activity of Nrf-2, Catalase and Superoxide dismutase (SOD) were reduced together with an enhanced expression of IL-1β, tumor necrosis factor-α (TNF-α), IL-6, and cyclooxygenase 2 (COX-2). Furthermore, Bcl-2, Caspase-3 and Caspase-8 expression were down-regulated while Bax was up-regulated by H2O2 stimulus. Aloe extract blunted the oxidative stress-induced inflammatory cascade triggered by H2O2 and modulated apoptosis. Aloe extract defends HCE cells from H2O2-induced injury possibly due its antioxidant and anti-inflammatory activity, indicating that eye drops containing aloe extract may be used as an adjunctive treatment for FECD.
Roles of mTOR in Diabetic Kidney Disease
Authors: Yasuda-Yamahara Mako , Kume Shinji , Maegawa Hiroshi
Published: February 2021

Diabetic kidney disease (DKD) is the leading cause of end-stage renal disease and the number of patients affected is increasing worldwide. Thus, there is a need to establish a new treatment for DKD to improve the renal prognosis of diabetic patients. Recently, it has shown that intracellular metabolic abnormalities are involved in the pathogenesis of DKD. In particular, the activity of mechanistic target of rapamycin complex 1 (mTORC1), a nutrient-sensing signaling molecule, is hyperactivated in various organs of diabetic patients, which suggests the involvement of excessive mTORC1 activation in the pathogenesis of diabetes. In DKD, hyperactivated mTORC1 may be involved in the pathogenesis of podocyte damage, which causes proteinuria, and tubular cell injury that decreases renal function. Therefore, elucidating the role of mTORC1 in DKD and developing new therapeutic agents that suppress mTORC1 hyperactivity may shed new light on DKD treatments in the future.
Actin Cytoskeleton Regulation by the Yeast NADPH Oxidase Yno1p Impacts Processes Controlled by MAPK Pathways
Authors: Weber Manuela , Basu Sukanya , González Beatriz , Greslehner Gregor P. , Singer Stefanie , Haskova Danusa , Hasek Jiri , Breitenbach Michael , W.Gourlay Campbell , Cullen Paul J. , Rinnerthaler Mark
Published: February 2021

Reactive oxygen species (ROS) that exceed the antioxidative capacity of the cell can be harmful and are termed oxidative stress. Increasing evidence suggests that ROS are not exclusively detrimental, but can fulfill important signaling functions. Recently, we have been able to demonstrate that a NADPH oxidase-like enzyme (termed Yno1p) exists in the single-celled organism Saccharomyces cerevisiae. This enzyme resides in the peripheral and perinuclear endoplasmic reticulum and functions in close proximity to the plasma membrane. Its product, hydrogen peroxide, which is also produced by the action of the superoxide dismutase, Sod1p, influences signaling of key regulatory proteins Ras2p and Yck1p/2p. In the present work, we demonstrate that Yno1p-derived H2O2 regulates outputs controlled by three MAP kinase pathways that can share components: the filamentous growth (filamentous growth MAPK (fMAPK)), pheromone response, and osmotic stress response (hyperosmolarity glycerol response, HOG) pathways. A key structural component and regulator in this process is the actin cytoskeleton. The nucleation and stabilization of actin are regulated by Yno1p. Cells lacking YNO1 showed reduced invasive growth, which could be reversed by stimulation of actin nucleation. Additionally, under osmotic stress, the vacuoles of a ∆yno1 strain show an enhanced fragmentation. During pheromone response induced by the addition of alpha-factor, Yno1p is responsible for a burst of ROS. Collectively, these results broaden the roles of ROS to encompass microbial differentiation responses and stress responses controlled by MAPK pathways.
Antioxidant Effect of a Probiotic Product on a Model of Oxidative Stress Induced by High-Intensity and Duration Physical Exercise
Authors: Sánchez Macarro Maravillas , Ávila-Gandía Vicente , Pérez-Piñero Silvia , Cánovas Fernando , García-Muñoz Ana María , Abellán-Ruiz María Salud , Victoria-Montesinos Desirée , Luque-Rubia Antonio J. , Climent Eric , Genovés Salvador , Ramon Daniel , Chenoll Empar , López-Román Francisco Javier
Published: February 2021

This randomized double-blind and controlled single-center clinical trial was designed to evaluate the effect of a 6-week intake of a probiotic product (1 capsule/day) vs. a placebo on an oxidative stress model of physical exercise (high intensity and duration) in male cyclists (probiotic group, n = 22; placebo, n = 21). This probiotic included three lyophilized strains (Bifidobacterium longum CECT 7347, Lactobacillus casei CECT 9104, and Lactobacillus rhamnosus CECT 8361). Study variables were urinary isoprostane, serum malondialdehyde (MDA), serum oxidized low-density lipoprotein (Ox-LDL), urinary 8-hydroxy-2′-deoxiguanosine (8-OHdG), serum protein carbonyl, serum glutathione peroxidase (GPx), and serum superoxide dismutase (SOD). At 6 weeks, as compared with baseline, significant differences in 8-OHdG (Δ mean difference −10.9 (95% CI −14.5 to −7.3); p < 0.001), MDA (Δ mean difference −207.6 (95% CI −349.1 to −66.1; p < 0.05), and Ox-LDL (Δ mean difference −122.5 (95% CI −240 to −4.5); p < 0.05) were found in the probiotic group only. Serum GPx did not increase in the probiotic group, whereas the mean difference was significant in the placebo group (477.8 (95% CI 112.5 to 843.2); p < 0.05). These findings suggest an antioxidant effect of this probiotic on underlying interacting oxidative stress mechanisms and their modulation in healthy subjects. The study was registered in ClinicalTrials.gov (NCT03798821).