bluuebunny/crossref_metadata_2025 huggingface dataset:
Twisted Nanographenes with Robust Conformational Stability
Authors: Song Penghui , Hashikawa Yoshifumi , Chaolumen
Published: October 2024

Owing to a lack of methodology for rationally and selectively synthesizing twisted nanographenes, it is usually inevitable that we obtain nanographenes as a mixture with various geometries, such as unidirectionally twisted, alternatively twisted, randomly twisted, and even wavy structures, reflecting the high activation barriers among them. Otherwise, they are interconvertible if the barriers are low enough such that only averaged properties can be observed under a thermal equilibrium. Recently, we reported on a double-twisted nanographene containing four [6]helicene units within the skeleton. In this paper, we discuss the robust conformational stability of the nanographene, both experimentally and computationally. The results indicate that the nanographene could only be racemized at temperatures exceeding 200 °C, and the first flip of one of the four [6]helicene units is the rate-degerming step.
Molecular Dynamic Simulation of Primary Damage with Electronic Stopping in Indium Phosphide
Authors: Bai Yurong , Liao Wenlong , Chen Zhongcun , Li Wei , Liu Wenbo , He Huan , He Chaohui
Published: October 2024

Indium phosphide (InP) is an excellent material used in space electronic devices due to its direct band gap, high electron mobility, and high radiation resistance. Displacement damage in InP, such as vacancies, interstitials, and clusters, induced by cosmic particles can lead to the serious degradation of InP devices. In this work, the analytical bond order potential of InP is modified with the short-range repulsive potential, and the hybrid potential is verified for its reliability to simulate the atomic cascade collisions. By using molecular dynamics simulations with the modified potential, the primary damage defects evolution of InP caused by 1–10 keV primary knock-on atoms (PKAs) are studied. The effects of electronic energy loss are also considered in our research. The results show that the addition of electronic stopping loss reduces the number of point defects and weakens the damage regions. The reduction rates of point defects caused by electronic energy loss at the stable state are 32.2% and 27.4% for 10 keV In-PKA and P-PKA, respectively. In addition, the effects of electronic energy loss can lead to an extreme decline in the number of medium clusters, cause large clusters to vanish, and make the small clusters dominant damage products in InP. These findings are helpful to explain the radiation-induced damage mechanism of InP and expand the application of InP devices.
The Multiple Challenges of Nutritional Microbiome Research During COVID-19—A Perspective and Results of a Single-Case Study
Authors: Donkers Anna , Seel Waldemar , Klümpen Linda , Simon Marie-Christine
Published: October 2024

The global coronavirus disease 2019 (COVID-19) pandemic has affected multiple aspects of people’s lives, which may also influence the results of studies conducted during this period across diverse research domains. This particularly includes the field of nutritional science, investigating the gut microbiota as a potential mediator in the association between dietary intake and health-related outcomes. This article identifies the challenges currently facing this area of research, points out potential solutions, and highlights the necessity to consider a range of issues when interpreting trials conducted during this period. Some of these issues have arisen specifically because of the measures implemented to interrupt the spread of small acute respiratory syndrome coronavirus 2 (SARS-CoV-2), while others remain relevant beyond the pandemic.
Malnutrition and Fall Risk in Older Adults: A Comprehensive Assessment Across Different Living Situations
Authors: Mziray Marzanna , Nowosad Karolina , Śliwińska Aleksandra , Chwesiuk Mateusz , Małgorzewicz Sylwia
Published: October 2024

Background: Malnutrition among older adults is associated with numerous adverse effects, including increased morbidity, mortality, prolonged hospital stays, and a heightened risk of falls. This study aims to investigate the prevalence of malnutrition in different groups of older adults using the F-MNA, anthropometry, and s-albumin and the association between nutritional status and fall risk. Methods: A total of 228 participants aged 60 years and older were divided into three groups: (1) patients in an internal medicine ward, (2) individuals living in family homes, and (3) residents of care homes. Disease profiles, nutritional status (assessed using the F-MNA and SNAQ), body composition, fall risk, and biochemical markers were evaluated. Results: The results indicated the highest prevalence of malnutrition among hospitalized individuals. Fall risk was associated with age, calf circumference, the F-MNA, the SNAQ, serum albumin levels, residence in a care home, comorbidities, and the number of medications taken daily. Regression analysis revealed that age, calf circumference, and residence in a care home were independent predictors of fall risk in older adults. Conclusion: Older adults are at significant risk of malnutrition, with the risk notably increasing during hospitalization and long-term stays in care homes. Hospitalized individuals had the poorest nutritional status and were at significant risk of further weight loss, underscoring the importance of post-discharge care and rehabilitation.
Association Between Maternal Weight Perception Before and During Pregnancy and Postpartum Depression Status in Southern China
Authors: Zhang Qin , Qiu Menglu , Guo Laidi , Li Yuanyuan , Xie Zhencheng , Yang Wanyi , Ouyang Lishan , Yin Jia , Zhou Yun , Fu Minghan , Ding Ye , Wang Zhixu
Published: October 2024

Objective: Postpartum depression (PPD) is a common complication after childbirth. Weight misperception can lead to self-esteem issues and mental health problems, especially in women and adolescents. The aim of this study was to investigate the association between weight perception before and during pregnancy and the status of PPD in Southern China. Methods: From October 2021 to November 2023, a multi-stage sampling method was used to recruit 2169 eligible mothers aged 18–49 who had delivered live-born singleton infants within 3 to 180 days postpartum. Anthropometric measurements and face-to-face questionnaire surveys were conducted to collect data. The Kappa test was used to assess the agreement between actual and perceived weight. The generalized linear model incorporating multiplicative interaction analysis was applied to explore the associations among variables. Results: The prevalence of PPD status was 18.0%. Among women, 35.2% perceived their pre-pregnancy weight (PPW) as abnormal, while 33.1% perceived their gestational weight gain (GWG) as inappropriate. There was poor agreement between maternal actual and perceived PPW/GWG (Kappa = 0.366, p < 0.001; Kappa = 0.188, p < 0.001), with 27.8% of women misperceiving their PPW and 52.1% misperceiving their GWG. The results of the general linear model indicated that women who perceived their PPW as underweight (β = 0.70, p = 0.016) or overweight/obese (β = 0.86, p < 0.001), as well as those who perceived their GWG as excessive (β = 0.47, p = 0.028) were more likely to exhibit PPD status. The interaction analysis results showed that those who perceived their PPW as underweight and their GWG as insufficient (β = 1.75, p = 0.020), as well as those who perceived their PPW as overweight/obese and their GWG as excessive (β = 0.90, p = 0.001) had a positive interactive effect on the occurrence of PPD status, while underestimating PPW and GWG may be a protective factor against PPD status (β = −1.03, p = 0.037). Conclusion: These findings support that maternal weight perception plays a role in the development of PPD status. Further improvement is needed in personalized health education for weight management, both prior to and throughout the pregnancy period. This can help women reduce weight anxiety, better understand their body image, and potentially lower the risk of developing PPD.
The Microbial Perspective: A Systematic Literature Review on Hypertension and Gut Microbiota
Authors: Tsiavos Alexandros , Antza Christina , Trakatelli Christina , Kotsis Vasilios
Published: October 2024

Background: Understanding the causes of hypertension is important in order to prevent the disease. Gut microbiota (GM) seems to play an important role, but the detailed physiology remains elusive, with alpha diversity being the most studied indicator. Objectives: This review aimed to systematically synthesize data on gut microbiota (alpha diversity) and hypertension. Methods: Databases, including MEDLINE/PubMed, Scopus, and EMBASE, and citations were systematically queried. We retrieved articles reporting the association between gut microbiota and hypertension. A valid critical appraisal tool was also used to investigate the quality of the included studies. Results: Eighteen eligible studies met our inclusion criteria. In this report, we focused on the following indices of alpha diversity: Shannon, Chao1, Simpson, and Abundance-based Coverage Estimator (ACE) indices. Several studies observed a significantly lower Shannon index in hypertensive patients compared to the healthy control group. Nevertheless, no statistically significant difference was found for the Chao1, Simpson, and ACE indices between hypertensive patients and controls. A higher Firmicutes-to-Bacteroidetes ratio (F/B ratio) was consistently observed in hypertensive patients compared to healthy controls, indicating potential dysbiosis in the gut microbiota. Conclusions: Our systematic review indicates that hypertensive patients may exhibit an imbalance in gut microbiota, evidenced by decreased alpha diversity and an elevated F/B ratio. However, the absence of statistically significant differences in secondary diversity indices (Chao1, Simpson, and ACE) highlights the need for further research. Well-designed, large-scale studies are necessary to clarify these associations and explore the role of gut microbiota in hypertension development.
Integrated Cardiorespiratory Rehabilitation and Its Impact on Cardio–Renal–Metabolic Profile After Cardiac Surgery
Authors: Marek-Iannucci Stefanie , Palazzuoli Alberto , Babarto Matteo , Lazarevic Zlatan , Beltrami Matteo , Fedele Francesco
Published: October 2024

Background: Cardiovascular diseases (CVDs) and chronic kidney disease (CKD) are common causes of morbidity and mortality. However, the impact of changes in lifestyle and rehabilitation programs on the progression of cardiovascular, renal, and metabolic (CRM) conditions, remains unclear. Methods: In a retrospective manner, we analyzed charts of 200 patients admitted for cardiorespiratory rehabilitation at our facility in 2023. A 6 min walk test, echocardiographic features, and laboratory values were investigated to evaluate the impact of cardiorespiratory rehabilitation in patients post cardiac surgery. This study examined the impact of combined lifestyle and exercise scores (diet, alcohol consumption, smoking, aerobic physical activity, sedentary behavior, sleep duration, and social connection) on cardio–renal–metabolic profiles and on a quality-of-life score measured by the Borg Scale. Results: During the rehabilitation program, left ventricular ejection fraction (LVEF) significantly increased (51.2 vs. 54.3%, SEM 0.51 p = 0.001). The six-minute walk test (6 MWT) significantly improved in terms of meters (133 vs. 373 m, SEM 6.41, p < 0.001) and Borg scale (6.6 vs. 2.5, SEM 0.06, p < 0.001). Glycemia levels reduced significantly (114.5± vs. 107.4± mg/dL, SEM 2.45, p = 0.001). While total cholesterol levels (119.4 vs. 129.6 mg/dL, SEM 2.4, p < 0.001) as well as HDL levels (29.9 vs. 40 mg/dL, SEM 0.62, p < 0.001) significantly increased, triglyceride levels significantly decreased (128.5 vs. 122.1 mg/dL, SEM 3.8, p = 0.048). There was no change in LDL levels. Creatinine levels remained stable throughout the period of rehabilitation. Conclusions: Cardiorespiratory rehabilitation has a significant impact on myocardial function, quality of life in terms of exercise capacity and symptoms (6 MWT) as well as laboratory levels relevant for cardiovascular prevention such as glycemia and lipid profile.
The Effect of Time-Restricted Eating on Cardiometabolic Risk Factors: A Systematic Review and Meta-Analysis
Authors: Panagiotou Krystalia , Stefanou Garyfallia , Kourlaba Georgia , Athanasopoulos Dimitrios , Kassari Penio , Charmandari Evangelia
Published: October 2024

Background/Objectives: Endogenous metabolic pathways periodically adjust with fluctuations in day and night, a biological process known as circadian rhythm. Time-restricted eating (TRE) aligns the time of food intake with the circadian rhythm. This study aims to investigate the effects of TRE on body weight, body composition and cardiometabolic risk factors. Methods: We reviewed articles from PubMed and Cochrane libraries for clinical trials that compare TRE with regular diet without calorie restriction. We conducted a meta-analysis of 26 studies. Results: Participants who followed TRE demonstrated reduction in body weight [mean-MD: −1.622 kg, (95% confidence interval (CI −2.302 to −0.941)], body mass index (BMI) [MD: −0.919 kg/m2 (95% CI: −1.189 to −0.650)], waist circumference [MD: −2.015 cm (95% CI: −3.212 to −0.819] and whole-body fat mass (WBFM) [MD: −0.662 kg (95% CI: −0.795 to −0.530)]. Improvements in cardiometabolic risk factors such as a decrease in insulin concentrations [MD: −0.458 mIU/L, (95% CI: −0.843 to −0.073)], total cholesterol [MD: −2.889 mg/dL (95% CI: −5.447 to −0.330) and LDL concentrations [MD: −2.717 mg/dL (95% CI: −4.412 to −1.021)] were observed. Conclusions: TRE is beneficial for weight loss and improvements in cardiometabolic risk factors. Further large-scale clinical trials are needed to confirm these findings.
Exploring the Relationship Between Dietary Habits and Perceptions of Mental and Physical Disorders, or a Sense of Accomplishment in Japan
Authors: Iwasa Tsuyoshi , Satoh Kouji , Hazama Makoto , Kagami-Katsuyama Hiroyo , Ito Naohito , Maeda-Yamamoto Mari , Nishihira Jun
Published: October 2024

Background. Japanese dietary patterns have traditionally focused on vegetables, legumes, and fish; however, in the last few quarters of the century, the consumption of meat, processed food, and ultra-processed food has become popular. It is anticipated that these changes in the Japanese dietary environment will increase the risk of developing psychosomatic disorders. Methods. In this study, we examined the relationship between dietary habits, psychosomatic disorders, and a sense of achievement. For men and women aged 20–80 years (n = 851) living in and around Ebetsu City, Hokkaido, Japan, a questionnaire on dietary habits over the past year, including mental and physical stress and a sense of achievement, was administered. The associations between dietary habits and psychosomatic disorders or a sense of achievement were analyzed using logistic regression (n = 654). Results. The associations between dietary habits and psychosomatic disorders differ depending on sex and age. We found that subjects who consumed more processed meat and ultra-processed food reported more psychosomatic disorders. On the other hand, those who perceived a sense of achievement consumed higher amounts of vegetables, albeit with a lower fish intake. Conclusions. In this study, we demonstrated that dietary habits and food ingredients are associated with mental and physical stress and a sense of achievement. Fish, which is considered to be good for the body, was mostly consumed by subjects who experienced mental and physical stress. These data indicate that highly stressful conditions may encourage fish consumption, as the body needs to mitigate psychosomatic disorders.
Neuronutrition and Its Impact on Post-Stroke Neurorehabilitation: Modulating Plasticity Through Diet
Authors: Ciancarelli Irene , Morone Giovanni , Iosa Marco , Cerasa Antonio , Calabrò Rocco Salvatore , Tozzi Ciancarelli Maria Giuliana
Published: October 2024

The recovery of neurological deficits after ischemic stroke largely depends on the brain’s ability to reorganize its undamaged neuronal circuits and neuronal plasticity phenomena. The consolidated evidence highlights the involvement of the patient’s impaired nutritional conditions in post-stroke recovery and unsatisfying rehabilitative outcomes. Standardized nutritional protocols usually applied in hospitalized patients in a rehabilitation setting aim mainly to improve the general health conditions of patients, do not consider the high inter-individual variability in neurorehabilitation outcomes, and are not sufficiently modifiable to provide neuroprotective and restorative dietary patterns that could promote neuronal plasticity and functional recovery during neurorehabilitation. Neuronutrition, an emergent scientific field of neuroscience, represents a valid model of a personalized nutritional approach, assuring, for each patient, nutrients having antioxidant and anti-inflammatory properties, ensuring a balanced microbiota composition, and providing adequate neurotrophic support, essential for improving neuronal plasticity, brain functional recovery, and rehabilitative outcomes. In the present narrative review, we provide an overview of the current knowledge on neuronutrition as an adjuvant strategy of a personalized nutritional approach potentially effective in improving post-stroke neuroplasticity and neurorehabilitation by counteracting or at least limiting post-stroke oxidative/nitrosative stress, neuroinflammation, and gut–brain axis disturbance.
Elevation of NT-proBNP Levels in Pediatric and Young Adult Hematopoietic Stem Cell Transplant Patients with Endotheliopathy
Authors: Uchida Kimberly , Yuan Xiaomeng , McArthur Jennifer , Lassiter Rebekah , Pan Haitao , Keerthi Dinesh , Tsai Katherine , Avent Yvonne , Hines Melissa , Martinez Hugo R. , Qudeimat Amr , Ghafoor Saad
Published: October 2024

Background/Objectives: Hematopoietic stem cell transplantation (HSCT) in pediatric and young adult (YA) patients can lead to endotheliopathy, such as thrombotic microangiopathy (TMA), sinusoidal obstruction syndrome (SOS), and diffuse alveolar hemorrhage (DAH). Natriuretic peptides have been studied as markers of endotheliopathy and critical illness. We hypothesized that an elevation in NT-proBNP was associated with the development of endotheliopathy (DAH, SOS, or TMA) in the first 100 days following HSCT in pediatric and YA patients. Methods: IRB-exempt status was obtained. This retrospective case–control study reviewed HSCT at our institution from 2016 to 2020. Cases were selected based on an endotheliopathy diagnosis in the first 100 days after HSCT. Cases were matched with controls. Baseline and near-event NT-proBNP levels were compared between cases and matched controls. The effect of NT-proBNP levels on developing endotheliopathy was estimated using conditional logistic regression. Results: Sixty-two patients were included (31 cases, 31 controls). Near-event NT-proBNP was significantly higher in cases compared to controls (median: 473 vs. 187 pg/mL, p = 0.03, Wilcoxon rank–sum test), in contrast to comparison in baseline NT-proBNP (median: 86 vs. 86 pg/mL, p = 0.51). After adjusting for covariates, an association between near-event NT-proBNP and odds of developing endotheliopathy did not achieve statistical significance. However, trends from most common transplant indications suggested an association between an elevated near-event NT-proBNP level and endotheliopathy, particularly in acute lymphoblastic leukemia (ALL) patients. Conclusions: NT-proBNP should be studied further as a biomarker for endotheliopathy in pediatric and YA patients undergoing HSCT. This may be particularly relevant for patients undergoing HSCT for ALL.
Challenges and Opportunities in Managing Geriatric Depression: The Role of Personalized Medicine and Age-Appropriate Therapeutic Approaches
Authors: Jaros Agnieszka , Rybakowski Filip , Cielecka-Piontek Judyta , Paczkowska-Walendowska Magdalena , Czerny Bogusław , Kamińki Adam , Wafaie Mahmoud Elsorady Rasha , Bienert Agnieszka
Published: October 2024

The global aging population has experienced rapid growth in recent decades, leading to an increased prevalence of psychiatric disorders, particularly depression, among older adults. Depression in the geriatric population is often compounded by chronic physical conditions and various psychosocial factors, significantly impacting their quality of life. The main question raised in this review is as follows: how can personalized medicine and age-appropriate therapeutic approaches improve the management of geriatric depression? This paper explores the epidemiology of geriatric depression, highlighting the influence of gender, race, and socioeconomic status on its prevalence. The classification and diagnosis of geriatric depressive disorders, based on ICD-11 and DSM-5 criteria, reveal the complexity of managing these conditions in older adults. Personalized medicine (PM) emerges as a promising approach, focusing on tailoring treatments to the individual’s genetic, clinical, and environmental characteristics. However, the application of PM in this demographic faces challenges, particularly in the context of pharmaceutical forms. The need for age-appropriate drug delivery systems is critical, given the prevalence of polypharmacy and issues such as dysphagia among the older patients. This study emphasizes the importance of developing patient-centric formulations to enhance the effectiveness of personalized therapy in geriatric patients.
Revolutionizing Eye Care: Exploring the Potential of Microneedle Drug Delivery
Authors: Rojekar Satish , Parit Swapnali , Gholap Amol D. , Manchare Ajit , Nangare Sopan N. , Hatvate Navnath , Sugandhi Vrashabh V. , Paudel Keshav Raj , Ingle Rahul G.
Published: October 2024

Microneedle technology revolutionizes ocular drug delivery by addressing challenges in treating ocular diseases. This review explores its potential impact, recent advancements, and clinical uses. This minimally invasive technique offers precise control of drug delivery to the eye, with various microneedle types showing the potential to penetrate barriers in the cornea and sclera, ensuring effective drug delivery. Recent advancements have improved safety and efficacy, offering sustained and controlled drug delivery for conditions like age-related macular degeneration and glaucoma. While promising, challenges such as regulatory barriers and long-term biocompatibility persist. Overcoming these through interdisciplinary research is crucial. Ultimately, microneedle drug delivery presents a revolutionary method with the potential to significantly enhance ocular disease treatment, marking a new era in eye care.
Authentication and Quality Control of the Brazilian Traditional Herb ‘Carquejas’ (Baccharis Species) Using Morpho-Anatomy and Microscopy
Authors: Antunes Kevin Alves , Raman Vijayasankar , Perera Wilmer Hervet , Heiden Gustavo , Pontarolo Roberto , Farago Paulo Vitor , Khan Ikhlas Ahmed , Manfron Jane
Published: October 2024

This research investigates the morpho-anatomical characteristics of seven Baccharis species, namely B. articulata, B. milleflora, B. myriocephala, B. pentaptera, B. riograndensis, B. sagittalis and B. trimera. Commonly called carquejas, these species have aerial photosynthetic winged stems known as cladodes and are widely used traditionally to treat digestive and diuretic disorders. This study aimed to characterize these commonly misidentified species using morphological and microscopic techniques, including light and scanning electron microscopy coupled with energy-dispersive X-ray spectroscopy. Trichomes, the presence or absence of oil bodies, and a subepidermal collenchyma layer at the wing edge were identified as primary anatomical markers that can help differentiate the studied species.
Assessing Genetic Variability and Population Structure of Alnus glutinosa (Black Alder) in Kazakhstan Using SSR Markers
Authors: Nurtaza Aidana , Dyussembekova Damira , Shevtsov Alexandr , Islamova Symbat , Samatova Indira , Koblanova Saule , Borodulina Olga , Kakimzhanova Almagul
Published: October 2024

Alnus glutinosa plays a crucial role in flood control, riverbank stabilization, and water purification. Recognized for its ecological significance, it is listed in the Red Book of Kazakhstan. This study investigated the genetic variability of A. glutinosa populations in Kazakhstan, analyzing 78 trees from seven populations in the Bayanaul mountain forest massif and the northern Turgay regions using 12 SSR markers. The study identified an average of 6.3 alleles and 2.783 effective alleles, as well as observed and expected heterozygosities of 0.570 and 0.562, respectively, reflecting genetic diversity. Among the populations, KS1 (northern Turgay) and PVL3 (Bayanaul) displayed the highest diversity, while PVL5 (Bayanaul) showed slightly lower diversity. The analysis of molecular variance results indicated that 86% of the genetic diversity occurred within populations, with 14% attributed to differences between populations. A UPGMA tree based on Nei’s genetic distance revealed three distinct clusters, suggesting geographically structured genetic variability in A. glutinosa populations.
The WRKY Family Transcription Factor GmWRKY72 Represses Glyceollin Phytoalexin Biosynthesis in Soybean
Authors: Lin Jie , Monsalvo Ivan , Kwon Hyejung , Pullano Sarah , Kovinich Nik
Published: October 2024

Phytoalexins are plant defense metabolites that are biosynthesized transiently in response to pathogens. Despite that their biosynthesis is highly restricted in plant tissues, the transcription factors that negatively regulate phytoalexin biosynthesis remain largely unknown. Glyceollins are isoflavonoid-derived phytoalexins that have critical roles in protecting soybean crops from the oomycete pathogen Phytophthora sojae. To identify regulators of glyceollin biosynthesis, we used a transcriptomics approach to search for transcription factors that are co-expressed with glyceollin biosynthesis in soybean and stilbene synthase phytoalexin genes in grapevine. We identified and functionally characterized the WRKY family protein GmWRKY72, which is one of four WRKY72-type transcription factors of soybean. Overexpressing and RNA interference silencing of GmWRKY72 in the soybean hairy root system decreased and increased expression of glyceollin biosynthetic genes and metabolites, respectively, in response to wall glucan elicitor from P. sojae. A translational fusion with green fluorescent protein demonstrated that GFP-GmWRKY72 localizes mainly to the nucleus of soybean cells. The GmWRKY72 protein directly interacts with several glyceollin biosynthetic gene promoters and the glyceollin transcription factor proteins GmNAC42-1 and GmMYB29A1 in yeast hybrid systems. The results show that GmWRKY72 is a negative regulator of glyceollin biosynthesis that may repress biosynthetic gene expression by interacting with transcription factor proteins and the DNA of glyceollin biosynthetic genes.
New Data on the Distribution and Systematic Position of Marsupella minutissima, a Poorly Known Species from Gymnomitriaceae (Marchantiophyta)
Authors: Bakalin Vadim A. , Choi Seung Se , Fedosov Vladimir E. , Moon Myung-Ok , Fedorova Alina V.
Published: October 2024

Marsupella minutissima was recorded for the first time outside of Japan in Jeju-do in the Republic of Korea. The new data significantly improve the knowledge of the species distribution. An analysis of the phylogenetic affinities of the species based on nuclear ITS and plastid trnL-trnF regions showed that it is resolved within the clade corresponding to Marsupella sect. Ustulatae, which is where the most morphologically similar M. funckii and M. sprucei are placed. These tree taxa are well delimited geographically. When found within the same region, such as a mountain chain, they occupy different altitudinal belts. Our results showed clear molecular delimitation; however, we failed to estimate the affinities of M. minutissima within the section based on the available molecular data. A description, line-art illustrations and photographs of the collected materials are provided; data on the oil bodies of the species were obtained for the first time.
Biomass Productivity and Photosynthetic Activity in Ulva compressa (Chlorophyta) in Raceway Photobioreactors Under Stress Conditions
Authors: Robles-Carnero Victor , Sesmero Rafael , Figueroa Felix L.
Published: October 2024

Research in seaweed cultivation technologies aims to increase production and reduce costs, leading to more efficient and sustainable processes. In this study, we analyzed the outdoor production of Ulva compressa cultured in summertime at different stocking densities of 0.6, 0.8 and 1.0 kg Fresh weight (FW) m−2 in a raceway photobioreactor with 30 m2 surface (3000 L), and its relation to photosynthetic activity. Under the experimental conditions of high temperature (>28–30 °C) and pH > 9 in culture water, higher seaweed density resulted in lower specific growth rate. The biomass production has been related to photosynthetic activity by using in vivo chlorophyll a fluorescence. Dynamic photoinhibition was observed at noon, which was less severe in cultures with higher algal densities. However, photosynthesis recovered in the afternoon. Seaweeds that were acclimatized for a week to the conditions of 1.0 kg FW m−2 stocking density showed an increase in biomass growth and absence of photoinhibition compared to non-acclimatized thalli. In conclusion, the cultivation of U. compressa in a mid-scale raceway photobiorreactor under conditions of high irradiance and temperature and low nutrient input, exhibited the best photosynthetic performance and hence the highest growth rates for the highest culture density assayed (1.0 kg FW m−2).
HSCCC Straightforward Fast Preparative Method for Isolation of Two Major Cytotoxic Withanolides from Athenaea fasciculata (Vell.) I.M.C. Rodrigues & Stehmann
Authors: Marques André Mesquita , Brito Lavínia de Carvalho , Figueiredo Maria Raquel
Published: October 2024

Athenaea fasciculata belongs to the Solanaceae family and is a promising source of cytotoxic withanolides known as aurelianolides A and B. In the last years, the pharmacological studies of these aurelianolides on different leukemia cell lines have stimulated new studies on their potential as alternative candidates for new lead anticancer drugs. However, the obtention of these two pure compounds by traditional preparative is a costly and long time-consuming process, which is performed in several steps. This study aimed to propose a straightforward approach for isolating aurelianolides A and B using high-speed countercurrent chromatography (HSCCC). In this study, among 10 different solvent systems, the system composed of n-hexane/ethyl acetate/methanol/water 3:6:2:1 (v/v/v/v) was chosen for optimization. This HEMWat system was optimized to 4:8:2:4 (v/v/v/v) and chosen for HSCCC separation in a tail-to-head elution mode. After the HSCCC scale-up procedure, a withanolides mixture (200.0 mg) was separated within 160 min in a single-step purification process. In total, 78.9 mg of aurelianolide A (up to 95.0% purity) and 54.3 mg of aurelianolide B (up to 88.5% purity) was obtained by this fast sequential liquid–liquid partition process. The isolated withanolides were identified by 1H and 13C NMR spectroscopy (this method has proven to be faster and more efficient than classical procedures (CC and Prep-TLC)).
Phytochemicals and Functional Properties of Pitaya Juice Powders
Authors: Herrera Mayra Denise , Zegbe Jorge A. , Reveles-Torres Luis Roberto
Published: October 2024

Background: Crassulacean acid metabolism plants, such as Stenocereus spp., are climate warming-resilient crops used as food and for by-products elaboration in arid and semi-arid agroecosystems. A few studies on secondary metabolites have been conducted in pitayo fruit (PF), but there are no reports of these compounds in juice powders (JP) with (JPS) or without seeds (JPWS). This study was devoted to characterizing the juice powders (JPS and JPWS) of five pitayas with different flesh colors with regard to some phytochemical and functional attributes. Methods: The study was conducted with a completely random design with factorial arrangement in treatments (PF × JP). Results: Differences among pitayas were related to peel and flesh color attributes. Except for soluble dietary fiber, the remainder of the non-digestible carbohydrates were greater in JPS than in JPWS of all pitayas. Phenols and flavonoids were found to be the highest in the JPWS of all pitayas, whereas total saponins were the highest in JPS of the ‘Pink’ pitaya. The JPWS of the ‘Yellow’ and ‘Reddish-Purple’ pitayas had the highest content of betaxanthins and indicaxanthins, respectively. Antioxidant capacity was the highest in JPS of ‘Reddish-Purple’ and ‘Pink’ pitayas. Conclusions: Except for some phenolic compounds, the study suggests that pitayas’ JPS would benefit human health when freshly consumed or as elaborated by-products.
Floral Developmental Morphology and Biochemical Characteristics of Male Sterile Mutants of Lagerstroemia indica
Authors: Deng Fuyuan , Lu Liushu , Li Lu , Yang Jing , Chen Yi , Zeng Huijie , Li Yongxin , Qiao Zhongquan
Published: October 2024

Male sterility is a common phenomenon in higher plants and often plays an important role in the selection of superior offspring. ‘Xiang Yun’ is a mutant of Lagerstroemia indica that does not bear fruit after flowering, and its flowering period is significantly longer than that of normal L. indica. To explore the timing and molecular mechanisms of sterility in ‘Xiang Yun’, this study determined the period of sterility through anatomical observation and compared the content of nutrients and the activity of antioxidative enzymes at different stages of flower development. Finally, sequence alignment and qPCR were used to analyze the differences in pollen development genes between ‘Xiang Yun’ and ‘Hong Ye’. The results showed that the anthers of ‘Xiang Yun’ dispersed pollen normally, but the pollen grains could not germinate normally. Observations with scanning electron microscopy revealed that the pollen grains were uneven in size and shriveled in shape. Further observation of anther sections found that abnormal development of the microspores began at the S2 stage, with the callose wall between microspores of ‘Xiang Yun’ being thicker than that of ‘Hong Ye’. In addition, during the flower development of ‘Xiang Yun’, the contents of soluble sugar, soluble protein, free proline, and triglycerides were deficient to varying degrees, and the activities of POD, SOD, and MDA were lower. Sequence alignment and qPCR showed that there were several mutations in EFD1, TPD1, and DEX1 of ‘Xiang Yun’ compared with ‘Hong Ye’, and the expression levels of these genes were abnormally elevated in the later stages of development. Our results clarified the timing and phenotype of male sterility in ‘Xiang Yun’. This provides solid and valuable information for further research on the molecular mechanism of sterility in ‘Xiang Yun’ and the genetic breeding of crape myrtle.
Novel Aryl Phosphate for Improving Fire Safety and Mechanical Properties of Epoxy Resins
Authors: Xu Yue , Zhang Wenjia , Yin Ru , Sun Jun , Li Bin , Liu Lubin
Published: October 2024

Epoxy resins (EPs) are highly flammable, and traditional flame retardant modifications often lead to a significant reduction in their mechanical performance, limiting their applications in aerospace and electrical and electronic fields. In this study, a novel flame retardant, bis(4-(((diphenylphosphoryl)oxy)methyl)phenyl)phenyl phosphate (DMP), was successfully prepared and introduced into the EP matrix. When the addition of DMP was 9 wt%, the EP/9 wt% DMP thermosets passed the UL-94 V-0 rating, and their LOI was increased from 24.5% of EP to 35.0%. With the introduction of DMP, the phosphoric acid compounds from the decomposition of DMP promoted the dehydration and charring of the EP matrix, and the compact, dense char layer effectively exerted the shielding effect in the condensed phase. Meanwhile, the produced phosphorus-containing radicals played a quenching effect in the gas phase. As a result, the peak heat release rate (PHRR) and total heat release (THR) of EP/9 wt% DMP were reduced by 68.9% and 18.1% compared to pure EP. In addition, the polyaromatic structure of DMP had good compatibility with the EP matrix, and the tensile strength, flexural strength and impact strength of EP/9 wt% DMP were enhanced by 116.38%, 17.84% and 59.11% in comparison with that of pure EP. This study is valuable for expanding the application of flame-retardant EP/DMP thermosets in emerging fields.
Comparative Proteome-Wide Abundance Profiling of Yeast Strains Deleted for Cdc48 Adaptors
Authors: Rossio Valentina , Paulo Joao A.
Published: October 2024

The yeast ATPase Cdc48 (known as p97/VCP in human cells) plays an important role in the Ubiquitin Proteasome System. VCP is essential for cancer cell proliferation, and its dysregulation has been implicated in several neurodegenerative diseases. Cdc48 functions by extracting ubiquitylated proteins from membranes, protein complexes and chromatin by often facilitating their proteasomal degradation. Specific adaptors or cofactors, primarily belonging to the UBX domain-containing protein family (which has seven members in Saccharomyces cerevisiae) recruit Cdc48 to ubiquitylated proteins. Here, we employed sample multiplexing-based quantitative mass spectrometry to profile global protein abundance in p97 adaptor deletion strains, specifically comparing seven single deletion strains of UBX domain-containing proteins and the Cuz1 deletion strain, which belongs to the zinc finger AN1-type domain protein family. We observed that each strain showed unique sets of differentially abundant proteins compared to the wild type. Our analysis also revealed a role for Ubx3 in maintaining wild type levels of mitochondrial proteins. Overall, we identified ~1400 differentially abundant proteins in the absence of a specific Cdc48 adaptor. This unique dataset offers a valuable resource for studying the functions of these adaptors, aiming to achieve a better understanding of the cellular processes regulated by Cdc48 itself and to deepen our understanding of the Ubiquitin Proteasome System.
Undisciplining the Museum: Indigenous Relationality as Religion
Authors: Mendoza Rebecca J.
Published: October 2024

What does it mean to decolonize or undiscipline the anthropology museum? What happens when the museum is confronted by Indigenous and descendant communities who demand an ethic of care rooted in relational ontologies and epistemologies? This article features Indigenous creativity as it has disrupted ‘business as usual’ in anthropology museums. This is primarily evidenced by Fork Peck Tribes who confronted the University of Montana to enact a long-overdue repatriation. Additional examples from The Metropolitan Museum of Art and Peabody Museum of Archaeology and Ethnology at Harvard demonstrate diverse expressions of relationality among Indigenous and descendant communities. These interventions are analyzed through Critical Indigenous Theory to specify the ways in which Indigenous religious traditions refuse the narratives and norms of settler colonial knowledge production and undermine the imperial museological practices of preservation. Instead, relationality is prioritized in the caretaking of and connection with more-than-human entities and materials in the museum. This article emphasizes relationality and repatriation as religious acts that challenge assumptions embedded in imperial and settler colonial approaches to history and science. From various social locations and through multiple strategies, we see the active undisciplining of the museum by Indigenous and descendant communities.
Beyond the Harvard Definition: A Critique of Josef Seifert’s Realistic Phenomenological Approach to Brain Death
Authors: Kovács Gusztáv
Published: October 2024

This article explores the significance of context in defining death, particularly through the lens of the Harvard definition and the critiques offered by Josef Seifert. The Harvard definition, established in 1968, is examined within its medical context, highlighting how it reflects pragmatic interests and raises critical questions about the boundaries between life and death. Seifert’s critiques focus on the reductionist nature of equating personhood with consciousness, emphasizing the need for a broader understanding of life indicators beyond mere physiological functions. The article also discusses the social implications of medicalization in the dying process. Furthermore, it addresses the challenges posed by the cessation of brain function as a criterion for death, contrasting it with more observable signs of life such as breathing and heartbeat. Ultimately, the article argues for a more nuanced understanding of death that incorporates social, cultural, and ethical dimensions, advocating for ongoing dialogue and potential revisions of death definitions to reflect the complexities of human experience. This comprehensive approach aims to bridge the gap between medical definitions and the lived realities of individuals facing death.
Transforming Post-Apartheid South Africa Through Shared Religious Education
Authors: Davids Nuraan
Published: October 2024

Ideas about shared religious education are in need of further exploration in post-apartheid South Africa. This is necessary, considering the contributions from faith communities in their shared resistance to apartheid. While some sectors of the Christian community, and particularly the Dutch Reformed Church provided a religious justification for apartheid, other denominations, together with Muslim, Jewish, and Hindu communities struggled against this white supremacist ideology. In other ways, the neglect of the potential of shared religious education provides an apt commentary on how some within-faith communities responded to a democracy by retreating into faith identities, as made explicit in the proliferation of faith-based schools. It follows, however, that if religious communities can mobilise together to resist the apartheid state, then it should be possible for these same communities to unite to work towards the kind of transformed society envisioned in their struggle against apartheid. Hence, the interest of this article: if faith-based schools are an inevitable manifestation of democratic and pluralistic societies, then what can these schools share in terms of content and ethos towards advancing democratic values? How might a shared religious education facilitate and sustain the reform measures, necessary for social transformation in South Africa?
Cartesian Trinitarian Persons
Authors: Farris Joshua R. , Hollingsworth Andrew
Published: October 2024

This paper explores the nature of Cartesian persons in relation to contemporary Social Trinitarian doctrines. We critically engage with three prominent models of the Social Trinity—those of William Lane Craig, Keith Yandell, and William Hasker—and examine how each defines the concept of personhood. Our analysis identifies the presence of a Cartesian notion of personhood (broadly defined) across all three models, though we argue that each presents unique challenges. Building on these critiques, we advance an alternative understanding of the Social Trinity that incorporates a distinct interpretation of Cartesian persons. Our proposal seeks to resolve tensions within current models and offer a more coherent account of Trinitarian personhood.
Exploring Acceptance of Agro-Biomass as Innovative Solution for Heating in Rural Areas in Romania
Authors: Török Ibolya , Mátyus Enikő , Sebestyén Tihamér-Tibor , Păunescu Carmen , Havadi-Nagy Kinga Xénia
Published: October 2024

This paper aims to examine what can predict the adoption of agro-biomass as an innovative heating solution in rural areas. It explores the social acceptance of agro-biomass such as agriculture byproducts for heating in a rural context. The objectives are threefold: (1) to examine if there is a causal relationship between awareness and knowledge of agro-biomass applications for heating and intention to adopt this solution; (2) to investigate the extent to which perceived local drivers as well as barriers associated with agro-biomass utilization influence the intention to adopt this heating solution; and (3) to determine if knowledge about agro-biomass energy use, as well as political barriers to agro-biomass adoption for heating, play a mediating and/or moderating role in the established causal relationships. This paper uses primary data collected from rural residents located in different geographic areas in Romania. The 673 valid responses were analysed through multiple regression and mediation and moderation tests. The results confirm that awareness, knowledge, and perception of local drivers and of political barriers are positive predictors of intention to adopt agro-biomass as an innovative solution for heating. They also confirm the mediating role of knowledge and the moderating role of political barriers in the established relationships.
Variations of Lake Ice Phenology Derived from MODIS LST Products and the Influencing Factors in Northeast China
Authors: Shi Xiaoguang , Cheng Jian , Yang Qian , Li Hongxing , Hao Xiaohua , Wang Chunxu
Published: October 2024

Lake ice phenology serves as a sensitive indicator of climate change in the lake-rich Northeast China. In this study, the freeze-up date (FUD), break-up date (BUD), and ice cover duration (ICD) of 31 lakes were extracted from a time series of the land water surface temperature (LWST) derived from the combined MOD11A1 and MYD11A1 products for the hydrological years 2001 to 2021. Our analysis showed a high correlation between the ice phenology measures derived by our study and those provided by hydrological records (R2 of 0.89) and public datasets (R2 > 0.7). There was a notable coherence in lake ice phenology in Northeast China, with a trend in later freeze-up (0.21 days/year) and earlier break-up (0.19 days/year) dates, resulting in shorter ice cover duration (0.50 days/year). The lake ice phenology of freshwater lakes exhibited a faster rate of change compared to saltwater lakes during the period from HY2001 to HY2020. We used redundancy analysis and correlation analysis to study the relationships between the LWST and lake ice phenology with various influencing factors, including lake properties, local climate factors, and atmospheric circulation. Solar radiation, latitude, and air temperature were found to be the primary factors. The FUD was more closely related to lake characteristics, while the BUD was linked to local climate factors. The large-scale oscillations were found to influence the changes in lake ice phenology via the coupled influence of air temperature and precipitation. The Antarctic Oscillation and North Atlantic Oscillation correlate more with LWST in winter, and the Arctic Oscillation correlates more with the ICD.