bluuebunny/crossref_metadata_2025 huggingface dataset:
Optimal Location and Sizing of DGs in DC Networks Using a Hybrid Methodology Based on the PPBIL Algorithm and the VSA
Authors: Grisales-Noreña Luis Fernando , Montoya Oscar Danilo , Hincapié-Isaza Ricardo Alberto , Granada Echeverri Mauricio , Perea-Moreno Alberto-Jesus
Published: August 2021

In this paper, we propose a master–slave methodology to address the problem of optimal integration (location and sizing) of Distributed Generators (DGs) in Direct Current (DC) networks. This proposed methodology employs a parallel version of the Population-Based Incremental Learning (PPBIL) optimization method in the master stage to solve the location problem and the Vortex Search Algorithm (VSA) in the slave stage to solve the sizing problem. In addition, it uses the reduction of power losses as the objective function, considering all the constraints associated with the technical conditions specific to DGs and DC networks. To validate its effectiveness and robustness, we use as comparison methods, different solution methodologies that have been reported in the specialized literature, as well as two test systems (the 21 and 69-bus test systems). All simulations were performed in MATLAB. According to the results, the proposed hybrid (PPBIL–VSA) methodology provides the best trade-off between quality of the solution and processing times and exhibits an adequate repeatability every time it is executed.
Spatial Discretization for Stochastic Semi-Linear Subdiffusion Equations Driven by Fractionally Integrated Multiplicative Space-Time White Noise
Authors: Wang Junmei , Hoult James , Yan Yubin
Published: August 2021

Spatial discretization of the stochastic semi-linear subdiffusion equations driven by fractionally integrated multiplicative space-time white noise is considered. The nonlinear terms f and σ satisfy the global Lipschitz conditions and the linear growth conditions. The space derivative and the fractionally integrated multiplicative space-time white noise are discretized by using the finite difference methods. Based on the approximations of the Green functions expressed by the Mittag–Leffler functions, the optimal spatial convergence rates of the proposed numerical method are proved uniformly in space under some suitable smoothness assumptions of the initial value.
Generative Design for Dimensioning of Retaining Walls
Authors: Díaz Gabriel , Herrera Rodrigo F. , Muñoz-La Rivera Felipe , Atencio Edison
Published: August 2021

The design of retaining walls follows a classic structure. The engineer proposes certain dimensions that will be modified until they comply with the regulatory and site restrictions presented by the project. This is an iterative process that can be optimized through a new method called generative design. The designer codes the characteristics and restrictions of the project so that the system creates the most appropriate solutions to the problem presented. In this research, a computer program was created to build the dimensions of retaining walls using generative design. For this purpose, Design Science Research (DSR) was used, complemented with the incremental software development method. A program that delivers multiple retaining wall design alternatives in a short time was constructed. The evaluation of this program was performed through usability tests, giving as main perceptions the program’s ease of use and the time savings concerning the traditional design.
A Novel Exact Plate Theory for Bending Vibrations Based on the Partial Differential Operator Theory
Authors: Zhou Chuanping , Wang Maofa , Han Xiao , Xue Huanhuan , Ni Jing , Zhou Weihua
Published: August 2021

Thick wall structures are usually applied at a highly reduced frequency. It is crucial to study the refined dynamic modeling of a thick plate, as it is directly related to the dynamic mechanical characteristics of an engineering structure or device, elastic wave scattering and dynamic stress concentration, and motion stability and dynamic control of a distributed parameter system. In this paper, based on the partial differential operator theory, an exact elasto-dynamics theory without assumptions for bending vibrations is presented by using the formal solution proposed by Boussinesq–Galerkin, and its dynamic equations are obtained under appropriate gauge conditions. The exact plate theory is then compared with other theories of plates. Since the derivation of the dynamic equation is conducted without any prior assumption, the proposed dynamic equation of plates is more exact and can be applied to a wider frequency range and greater thickness.
Some New Results for Jaggi-W-Contraction-Type Mappings on b-Metric-like Spaces
Authors: Mitrović Slobodanka , Parvaneh Vahid , De La Sen Manuel , Vujaković Jelena , Radenović Stojan
Published: August 2021

In this article, we generalize, improve, unify and enrich some results for Jaggi-W-contraction-type mappings in the framework of b-metric-like spaces. Our results supplement numerous methods in the existing literature, and we created new approach to prove that a Picard sequence is Cauchy in a b-metric-like space. Among other things, we prove Wardowski’s theorem, but now by using only the property (W1). Our proofs in this article are much shorter than ones in recently published papers.
Using DANP-mV Model to Improve the Paid Training Measures for Travel Agents amid the COVID-19 Pandemic
Authors: Liu Chui-Hua , Liu Bochner
Published: August 2021

Due to the COVID-19 pandemic bringing travel to a standstill, an initiative of paid training for travel agencies was launched by the government. The purpose of this research is to improve the effectiveness of this measure, which is fundamental to the next tourism crisis management. Based on the related theories of tourism crisis management, organizational learning, and behavior adaption, the DANP-mV model developed an evaluation system for examining the training measure. The result of an influential network relationship map (INRM) shows that using influential effects, the “policy object” dimension and its criterion “subsidiary” should be the first improvement priority. To effectively achieve the aspiration level, the gap values point out “training courses” dimension and its criterion “excellent services” are first to improve. An action plan is produced containing all the findings and made available for easy indexing. It may contribute to the current measure improvements. Therefore, this innovative approach could help decision makers with tourism crisis policy making and help the sector with evolving learning readiness to remain sustainable.
Updates in the Diagnosis and Management of Linear IgA Disease: A Systematic Review
Authors: Shin Leah , Gardner Jeffrey T. , Dao Harry
Published: August 2021

Background and Objectives: Linear IgA disease (LAD) is a rare autoimmune blistering disease with linear IgA deposits along the basement membrane zone. Direct immunofluorescence remains the gold standard for diagnosis, but other diagnostic measures reported in recent literature have proven useful in the setting of inconclusive preliminary results. Dapsone is a commonly used treatment, but many therapeutic agents have emerged in recent years. The objective of this study is to provide a comprehensive overview of updates on the diagnosis and management of LAD. Materials and Methods: A literature search was conducted from May to June of 2021 for articles published in the last 5 years that were related to the diagnosis and management of LAD. Results: False-negative results in cases of drug-induced LAD and the presence of IgG and IgM antibodies on immunofluorescence studies were reported. Serration pattern analysis has been reported to be useful in distinguishing LAD from sublamina densa-type LAD. Rituximab, omalizumab, etanercept, IVIg, sulfonamides, topical corticosteroids, and others have been used successfully in adult and pediatric patients with varying disease severity. Topical corticosteroids were preferred for pediatric patients while rituximab and IVIg were used in adults with recalcitrant LAD. Sulfonamides were utilized in places without access to dapsone. Conclusion: In cases where preliminary biopsy results are negative and clinical suspicion is high, repeat biopsy and additional diagnostic studies should be used. Patient factors such as age, medical comorbidities, and disease severity play a role in therapeutic selection.
Flexible 6-in-1 Microsensor for Real-Time Microscopic Monitoring of Proton Battery
Authors: Lee Chi-Yuan , Chen Chia-Hung , Yang Chin-Yuan , Cheong John-Shong , Chien Yun-Hsiu , Lin Yi-Chuan
Published: August 2021

According to the comparison between a proton battery and a proton exchange membrane fuel cell (PEMFC), the PEMFC requires oxygen and hydrogen for generating electricity, so a hydrogen tank is required, leading to larger volume of PEMFC. The proton battery can store hydrogen in the carbon layer, combined with the oxygen in the air to form water to generate electricity; thus, the battery cost and the space for a hydrogen tank can be reduced a lot, and it is used more extensively. As the proton battery is a new research area, multiple important physical quantities inside the proton battery should be further understood and monitored so as to enhance the performance of battery. The proton battery has the potential for practical applications, as well as water electrolysis, proton storage and discharge functions, and it can be produced without expensive metals. Therefore, in this study, we use micro-electro-mechanical systems (MEMS) technology to develop a diagnostic tool for the proton battery based on the developed microhydrogen sensor, integrated with the voltage, current, temperature, humidity and flow microsensors developed by this laboratory to complete a flexible integrated 6-in-1 microsensor, which is embedded in the proton battery to measure internal important physical parameters simultaneously so that the reaction condition in the proton battery can be mastered more accurately. In addition, the interaction of physical quantities of the proton battery are discussed so as to enhance the proton battery’s performance.
The Phase Structural Evolution and Gas Separation Performances of Cellulose Acetate/Polyimide Composite Membrane from Polymer to Carbon Stage
Authors: Li Haojie , Xu Shan , Zhao Bingyu , Yu Yuxiu , Liu Yaodong
Published: August 2021

Blending and heat-treatment play significant roles in adjusting gas separation performances of membranes, especially for incorporating thermally labile polymers into carbon molecular sieve membranes (CMSMs). In this work, cellulose acetate (CA) is introduced into polyimide (PI) as a sacrificial phase to adjust the structure and gas separation performance from polymer to carbon. A novel result is observed that the gas permeability is reduced, even when the immiscible CA phase decomposes and forms pores after heat treatment at 350 °C. After carbonization at 600 °C, the miscible CA has changed without contribution, while the role of the immiscible CA phase has changed from original hindrance to facilitation, the composite-based CMSM at a CA content of 10 wt.% shows highest performances, a H2 permeability of ~5300 Barrer (56% enhancement) with a similar H2/N2 permselectivity of 42. The structural analyses reveal that the chain interactions and phase separation behaviors between CA and PI play critical roles on membrane structures and gas diffusion, and the corresponding phase structural evolutions during heat treatment and carbonization determine gas separation properties.
Trefoil Factor Family Member 2: From a High-Fat-Induced Gene to a Potential Obesity Therapy Target
Authors: Ghanemi Abdelaziz , Yoshioka Mayumi , St-Amand Jonny
Published: August 2021

Obesity has its epidemiological patterns continuously increasing. With controlling both diet and exercise being the main approaches to manage the energy metabolism balance, a high-fat (HF) diet is of particular importance. Indeed, lipids have a low satiety potential but a high caloric density. Thus, focusing on pharmacologically targetable pathways remains an approach with promising therapeutic potential. Within this context, trefoil factor family member 2 (Tff2) has been characterized as specifically induced by HF diet rather than low-fat diet. TFF2 has also been linked to diverse neurological mechanisms and metabolic patterns suggesting its role in energy balance. The hypothesis is that TFF2 would be a HF diet-induced signal that regulates metabolism with a focus on lipids. Within this review, we put the spotlight on key findings highlighting this line of thought. Importantly, the hypothetical mechanisms pointed highlight TFF2 as an important contributor to obesity development via increasing lipids intestinal absorption and anabolism. Therefore, an outlook for future experimental activities and evaluation of the therapeutic potential of TFF2 inhibition is given. Indeed, its knockdown or downregulation would contribute to an antiobesity phenotype. We believe this work represents an addition to our understanding of the lipidic molecular implications in obesity, which will contribute to develop therapies aiming to manage the lipidic metabolic pathways including the absorption, storage and metabolism via targeting TFF2-related pathways. We briefly discuss important relevant concepts for both basic and clinical researchers.
Detection of Lung Cancer via Blood Plasma and 1H-NMR Metabolomics: Validation by a Semi-Targeted and Quantitative Approach Using a Protein-Binding Competitor
Authors: Derveaux Elien , Thomeer Michiel , Mesotten Liesbet , Reekmans Gunter , Adriaensens Peter
Published: August 2021

Metabolite profiling of blood plasma, by proton nuclear magnetic resonance (1H-NMR) spectroscopy, offers great potential for early cancer diagnosis and unraveling disruptions in cancer metabolism. Despite the essential attempts to standardize pre-analytical and external conditions, such as pH or temperature, the donor-intrinsic plasma protein concentration is highly overlooked. However, this is of utmost importance, since several metabolites bind to these proteins, resulting in an underestimation of signal intensities. This paper describes a novel 1H-NMR approach to avoid metabolite binding by adding 4 mM trimethylsilyl-2,2,3,3-tetradeuteropropionic acid (TSP) as a strong binding competitor. In addition, it is demonstrated, for the first time, that maleic acid is a reliable internal standard to quantify the human plasma metabolites without the need for protein precipitation. Metabolite spiking is further used to identify the peaks of 62 plasma metabolites and to divide the 1H-NMR spectrum into 237 well-defined integration regions, representing these 62 metabolites. A supervised multivariate classification model, trained using the intensities of these integration regions (areas under the peaks), was able to differentiate between lung cancer patients and healthy controls in a large patient cohort (n = 160), with a specificity, sensitivity, and area under the curve of 93%, 85%, and 0.95, respectively. The robustness of the classification model is shown by validation in an independent patient cohort (n = 72).
Significant Involvement of Double Diffusion Theories on Viscoelastic Fluid Comprising Variable Thermophysical Properties
Authors: Sohail Muhammad , Nazir Umar , Bazighifan Omar , El-Nabulsi Rami Ahmad , Selim Mahmoud M. , Alrabaiah Hussam , Thounthong Phatiphat
Published: August 2021

This report examines the heat and mass transfer in three-dimensional second grade non-Newtonian fluid in the presence of a variable magnetic field. Heat transfer is presented with the involvement of thermal relaxation time and variable thermal conductivity. The generalized theory for mass flux with variable mass diffusion coefficient is considered in the transport of species. The conservation laws are modeled in simplified form via boundary layer theory which results as a system of coupled non-linear partial differential equations. Group similarity analysis is engaged for the conversion of derived conservation laws in the form of highly non-linear ordinary differential equations. The solution is obtained vial optimal homotopy procedure (OHP). The convergence of the scheme is shown through error analysis. The obtained solution is displayed through graphs and tables for different influential parameters.
Potential Zoonotic Pathovars of Diarrheagenic Escherichia coli Detected in Lambs for Human Consumption from Tierra del Fuego, Argentina
Authors: Blanco Crivelli Ximena , Bonino María Paz , Sanin Mariana Soledad , Petrina Juan Facundo , Disalvo Vilma Noelia , Massa Rosana , Miliwebsky Elizabeth , Navarro Armando , Chinen Isabel , Bentancor Adriana
Published: August 2021

Diarrheagenic Escherichia coli (DEC) pathovars impact childhood health. The southern region of Argentina shows the highest incidence of hemolytic uremic syndrome (HUS) in children of the country. The big island of Tierra del Fuego (TDF) in Argentina registered an incidence of five cases/100,000 inhabitants of HUS in 2019. This work aimed to establish the prevalence of STEC, EPEC, and EAEC in lambs slaughtered in abattoirs from TDF as well as to characterize the phenotypes and the genotypes of the isolated pathogens. The prevalence was 26.6% for stx+, 5.7% for eae+, and 0.27% for aagR+/aaiC+. Twelve STEC isolates were obtained and belonged to the following serotypes: O70:HNT, O81:H21, O81:HNT, O102:H6, O128ab:H2, O174:H8, and O174:HNT. Their genotypic profiles were stx1c (2), stx1c/ehxA (3), stx2b/ehxA (1), stx1c/stx2b (2), and stx1c/stx2/ehxA (4). Six EPEC isolates were obtained and corresponded to five serotypes: O2:H40, O32:H8, O56:H6, O108:H21, and O177:H25. All the EPEC isolates were bfpA- and two were ehxA+. By XbaI-PFGE of 17 isolates, two clusters were identified. By antimicrobial susceptibility tests, 8/12 STEC and 5/6 EPEC were resistant to at least one antibiotic. This work provides new data to understand the ecology of DEC in TDF and confirms that ovine are an important carrier of these pathogens in the region.
Bacterially Delivered miRNA-Mediated Toll-like Receptor 8 Gene Silencing for Combined Therapy in a Murine Model of Atopic Dermatitis: Therapeutic Effect of miRTLR8 in AD
Authors: Yoon Wonsuck , Kim Eun-Jae , Park Yongsung , Kim Seunghyun , Park Yong-Keun , Yoo Young
Published: August 2021

In atopic dermatitis (AD), skin inflammation is caused by complex interactions between genetic disposition and aberrant innate/adaptive immune responses. Toll-like receptors (TLRs) are key molecules in the innate/adaptive immune response as they recognize various molecular motifs associated with pathogens. Among them, TLR8 is implicated in eczematous skin reactions. We investigated the combined therapeutic effects of TLR8 gene silencing by the bacterial delivery of miRNA. We used Salmonella as a vector to deliver TLR8 miRNA. The recombinant strain of Salmonella enterica subsp. enterica serovar Typhimurium (ST) expressing TLR8 miRNA (ST-miRTLR8) was prepared for knockdown of TLR8. After oral administration of ST-miRTLR8 into mice, we observed the cytokine levels, skin pathology and scratching behaviors in an AD-like mouse model. TLR8 down-regulation decreased macrophage-derived chemokine concentrations in activated human mast cells. Serum IgE and interleukin-4 production were suppressed whereas IFN-γ was induced after oral administration of ST-miRTLR8. Scratching behaviors and skin inflammation were also improved. In addition, attenuated S. typhimurium safely accumulated in mouse macrophages and showed adjuvant effects. This study shows that the recombinant miRNA that expresses the TLR8 miRNA has therapeutic effects by suppressing Th2 inflammation. TLR gene modulation using miRNA via Salmonella vectors will thus have a double-protective effect in the treatment of AD.
Effect of Methionine Supplementation on Rumen Microbiota, Fermentation, and Amino Acid Metabolism in In Vitro Cultures Containing Nitrate
Authors: Hassan Faiz-ul , Guo Yanxia , Li Mengwei , Tang Zhenhua , Peng Lijuan , Liang Xin , Yang Chengjian
Published: August 2021

This study evaluated the effect of methionine on in vitro methane (CH4) production, rumen fermentation, amino acid (AA) metabolism, and rumen microbiota in a low protein diet. We evaluated three levels of methionine (M0, 0%; M1, 0.28%; and M2, 1.12%) of in the presence of sodium nitrate (1%) in a diet containing elephant grass (90%) and concentrate (10%). We used an in vitro batch culture technique by using rumen fluid from cannulated buffaloes. Total gas and CH4 production were measured in each fermentation bottle at 3, 6, 9, 12, 24, 48, 72 h of incubation. Results revealed that M0 decreased (p < 0.001) the total gas and CH4 production, but methionine exhibited no effect on these parameters. M0 decreased (p < 0.05) the individual and total volatile fatty acids (VFAs), while increasing (p < 0.05) the ruminal pH, acetate to propionate ratio, and microbial protein content. Methionine did not affect ruminal AA contents except asparagine, which substantially increased (p = 0.003). M2 increased the protozoa counts, but both M0 and M1 decreased (p < 0.05) the relative abundance of Firmicutes while increasing (p < 0.05) the Campilobacterota and Proteobacteria. However, Prevotella and γ-Proteobacteria were identified as biomarkers in the nitrate group. Our findings indicate that methionine can increase ruminal asparagine content and the population of Compylobactor.
Cross-Shore and Depth Zonations in Bacterial Diversity Are Linked to Age and Source of Dissolved Organic Matter across the Intertidal Area of a Sandy Beach
Authors: Degenhardt Julius , Merder Julian , Heyerhoff Benedikt , Simon Heike , Engelen Bert , Waska Hannelore
Published: August 2021

Microbial communities and dissolved organic matter (DOM) are intrinsically linked within the global carbon cycle. Demonstrating this link on a molecular level is hampered by the complexity of both counterparts. We have now investigated this connection within intertidal beach sediments, characterized by a runnel-ridge system and subterranean groundwater discharge. Using datasets generated by Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) and Ilumina-sequencing of 16S rRNA genes, we predicted metabolic functions and determined links between bacterial communities and DOM composition. Four bacterial clusters were defined, reflecting differences within the community compositions. Those were attributed to distinct areas, depths, or metabolic niches. Cluster I was found throughout all surface sediments, probably involved in algal-polymer degradation. In ridge and low water line samples, cluster III became prominent. Associated porewaters indicated an influence of terrestrial DOM and the release of aromatic compounds from reactive iron oxides. Cluster IV showed the highest seasonality and was associated with species previously reported from a subsurface bloom. Interestingly, Cluster II harbored several members of the candidate phyla radiation (CPR) and was related to highly degraded DOM. This may be one of the first geochemical proofs for the role of candidate phyla in the degradation of highly refractory DOM.
Microbial Diversity Profiling of Gut Microbiota of Macropus giganteus Using Three Hypervariable Regions of the Bacterial 16S rRNA
Authors: O’Dea Christian , Huerlimann Roger , Masters Nicole , Kuballa Anna , Veal Cameron , Fisher Paul , Stratton Helen , Katouli Mohammad
Published: August 2021

Animal faecal contamination of surface waters poses a human health risk, as they may contain pathogenic bacteria or viruses. Of the numerous animal species residing along surface waterways in Australia, macropod species are a top contributor to wild animals’ faecal pollution load. We characterised the gut microbiota of 30 native Australian Eastern Grey Kangaroos from six geographical regions (five kangaroos from each region) within South East Queensland in order to establish their bacterial diversity and identify potential novel species-specific bacteria for the rapid detection of faecal contamination of surface waters by these animals. Using three hypervariable regions (HVRs) of the 16S rRNA gene (i.e., V1–V3, V3–V4, and V5–V6), for their effectiveness in delineating the gut microbial diversity, faecal samples from each region were pooled and microbial genomic DNA was extracted, sequenced, and analysed. Results indicated that V1-V3 yielded a higher taxa richness due to its larger target region (~480 bp); however, higher levels of unassigned taxa were observed using the V1-V3 region. In contrast, the V3–V4 HVR (~569 bp) attained a higher likelihood of a taxonomic hit identity to the bacterial species level, with a 5-fold decrease in unassigned taxa. There were distinct dissimilarities in beta diversity between the regions, with the V1-V3 region displaying the highest number of unique taxa (n = 42), followed by V3–V4 (n = 11) and V5–V6 (n = 8). Variations in the gut microbial diversity profiles of kangaroos from different regions were also observed, which indicates that environmental factors may impact the microbial development and, thus, the composition of the gut microbiome of these animals.
Mineralogical Prediction on the Flotation Behavior of Copper and Molybdenum Minerals from Blended Cu–Mo Ores in Seawater
Authors: Tanaka Yoshiyuki , Miki Hajime , Suyantara Gde , Aoki Yuji , Hirajima Tsuyoshi
Published: August 2021

The copper ore in Chilean copper porphyry deposits is often associated with molybdenum minerals. This copper–molybdenum (Cu–Mo) sulfide ore is generally mined from various locations in the mining site; thus, the mineral composition, oxidation degree, mineral particle size, and grade vary. Therefore, in the mining operation, it is common to blend the ores mined from various spots and then process them using flotation. In this study, the floatability of five types of Cu–Mo ores and the blending of these ores in seawater was investigated. The oxidation degree of these Cu–Mo ores was evaluated, and the correlation between flotation recovery and oxidation degree is presented. Furthermore, the flotation kinetics of each Cu–Mo ore were calculated based on a mineralogical analysis using mineral liberation analysis (MLA). A mineralogical prediction model was proposed to estimate the flotation behavior of blended Cu–Mo ore as a function of the flotation behavior of each Cu–Mo ore. The flotation results show that the recovery of copper and molybdenum decreased with the increasing copper oxidization degree. In addition, the recovery of blended ore can be predicted via the flotation rate equation, using the maximum recovery (Rmax) and flotation rate coefficient (k) determined from the flotation rate analysis of each ore before blending. It was found that Rmax and k of the respective minerals slightly decreased with increasing the degree of copper oxidation. Moreover, Rmax varied greatly depending on the mineral species. The total copper and molybdenum recovery were strongly affected by the degree of copper oxidation as the mineral fraction in the ore varied greatly depending upon the degree of oxidation.
Antimicrobial and Antibiofilm Activity of Curcumin-Loaded Electrospun Nanofibers for the Prevention of the Biofilm-Associated Infections
Authors: Di Salle Anna , Viscusi Gianluca , Di Cristo Francesca , Valentino Anna , Gorrasi Giuliana , Lamberti Elena , Vittoria Vittoria , Calarco Anna , Peluso Gianfranco
Published: August 2021

Curcumin extracted from the rhizome of Curcuma Longa has been used in therapeutic preparations for centuries in different parts of the world. However, its bioactivity is limited by chemical instability, water insolubility, low bioavailability, and extensive metabolism. In this study, the coaxial electrospinning technique was used to produce both poly (ε-caprolactone) (PCL)–curcumin and core–shell nanofibers composed of PCL and curcumin in the core and poly (lactic acid) (PLA) in the shell. Morphology and physical properties, as well as the release of curcumin were studied and compared with neat PCL, showing the formation of randomly oriented, defect-free cylindrical fibers with a narrow distribution of the dimensions. The antibacterial and antibiofilm potential, including the capacity to interfere with the quorum-sensing mechanism, was evaluated on Pseudomonas aeruginosa PAO1, and Streptococcus mutans, two opportunistic pathogenic bacteria frequently associated with infections. The reported results demonstrated the ability of the Curcumin-loading membranes to inhibit both PAO1 and S. mutans biofilm growth and activity, thus representing a promising solution for the prevention of biofilm-associated infections. Moreover, the high biocompatibility and the ability to control the oxidative stress of damaged tissue, make the synthesized membranes useful as scaffolds in tissue engineering regeneration, helping to accelerate the healing process.
Polyphenol Rich Ajuga bracteosa Transgenic Regenerants Display Better Pharmacological Potential
Authors: Rubnawaz Samina , Kayani Waqas , Akhtar Nosheen , Mahmood Rashid , Khan Asif , Okla Mohammad , Alamri Saud , Alaraidh Ibrahim , Alwasel Yasmeen , Mirza Bushra
Published: August 2021

Ajuga bracteosa Wall. ex Benth. is an endangered medicinal herb traditionally used against different ailments. The present study aimed to create new insight into the fundamental mechanisms of genetic transformation and the biological activities of this plant. We transformed the A. bracteosa plant with rol genes of Agrobacterium rhizogenes and raised the regenerants from the hairy roots. These transgenic regenerants were screened for in vitro antioxidant activities, a range of in vivo assays, elemental analysis, polyphenol content, and different phytochemicals found through HPLC. Among 18 polyphenolic standards, kaempferol was most abundant in all transgenic lines. Furthermore, transgenic line 3 (ABRL3) showed maximum phenolics and flavonoids content among all tested plant extracts. ABRL3 also demonstrated the highest total antioxidant capacity (8.16 ± 1 μg AAE/mg), total reducing power, (6.60 ± 1.17 μg AAE/mg), DPPH activity (IC50 = 59.5 ± 0.8 μg/mL), hydroxyl ion scavenging (IC50 = 122.5 ± 0.90 μg/mL), and iron-chelating power (IC50 = 154.8 ± 2 μg/mL). Moreover, transformed plant extracts produced significant analgesic, anti-inflammatory, anticoagulant, and antidepressant activities in BALB/c mice models. In conclusion, transgenic regenerants of A. bracteosa pose better antioxidant and pharmacological properties under the effect of rol genes as compared to wild-type plants.
Formation of Microcages from a Collagen Mimetic Peptide via Metal-Ligand Interactions
Authors: Gleaton Jeremy , Curtis Ryan W. , Chmielewski Jean
Published: August 2021

Here, the hierarchical assembly of a collagen mimetic peptide (CMP) displaying four bipyridine moieties is described. The CMP was capable of forming triple helices followed by self-assembly into disks and domes. Treatment of these disks and domes with metal ions such as Fe(II), Cu(II), Zn(II), Co(II), and Ru(III) triggered the formation of microcages, and micron-sized cup-like structures. Mechanistic studies suggest that the formation of the microcages proceeds from the disks and domes in a metal-dependent fashion. Fluorescently-labeled dextrans were encapsulated within the cages and displayed a time-dependent release using thermal conditions.
UV-C Peroxymonosulfate Activation for Wastewater Regeneration: Simultaneous Inactivation of Pathogens and Degradation of Contaminants of Emerging Concern
Authors: Berruti Ilaria , Nahim-Granados Samira , Abeledo-Lameiro María Jesús , Oller Isabel , Polo-López María Inmaculada
Published: August 2021

This study explores the capability of Sulfate Radical-based Advanced Oxidation Processes (SR-AOPs) for the simultaneous disinfection and decontamination of urban wastewater. Sulfate and hydroxyl radicals in solution were generated activating peroxymonosulfate (PMS) under UV-C irradiation at pilot plant scale. The efficiency of the process was assessed toward the removal of three CECs (Trimethoprim (TMP), Sulfamethoxazole (SMX), and Diclofenac (DCF)) and three bacteria (Escherichia coli, Enterococcus spp., and Pseudomonas spp.) in actual urban wastewater (UWW), obtaining the optimal value of PMS at 0.5 mmol/L. Under such experimental conditions, bacterial concentration ≤ 10 CFU/100 mL was reached after 15 min of UV-C treatment (0.03 kJ/L of accumulative UV-C radiation) for natural occurring bacteria, no bacterial regrowth was observed after 24 and 48 h, and 80% removal of total CECs was achieved after 12 min (0.03 kJ/L), with a release of sulfate ions far from the limit established in wastewater discharge. Moreover, the inactivation of Ampicillin (AMP), Ciprofloxacin (CPX), and Trimethoprim (TMP) antibiotic-resistant bacteria (ARB) and reduction of target genes (ARGs) were successfully achieved. Finally, a harmful effect toward the receiving aquatic environment was not observed according to Aliivibrio fischeri toxicity tests, while a slightly toxic effect toward plant growth (phytotoxicity tests) was detected. As a conclusion, a cost analysis demonstrated that the process could be feasible and a promising alternative to successfully address wastewater reuse challenges.
Green Tea Suppresses Brain Aging
Authors: Unno Keiko , Nakamura Yoriyuki
Published: August 2021

Epidemiological studies have demonstrated that the intake of green tea is effective in reducing the risk of dementia. The most important component of green tea is epigallocatechin gallate (EGCG). Both EGCG and epigallocatechin (EGC) have been suggested to cross the blood–brain barrier to reach the brain parenchyma, but EGCG has been found to be more effective than EGC in promoting neuronal differentiation. It has also been suggested that the products of EGCG decomposition by the intestinal microbiota promote the differentiation of nerve cells and that both EGCG and its degradation products act on nerve cells with a time lag. On the other hand, the free amino acids theanine and arginine contained in green tea have stress-reducing effects. While long-term stress accelerates the aging of the brain, theanine and arginine suppress the aging of the brain due to their anti-stress effect. Since this effect is counteracted by EGCG and caffeine, the ratios between these green tea components are important for the anti-stress action. In this review, we describe how green tea suppresses brain aging, through the activation of nerve cells by both EGCG and its degradation products, and the reductions in stress achieved by theanine and arginine.
Growth Temperature Influence on Atomic-Layer-Deposited In2O3 Thin Films and Their Application in Inorganic Perovskite Solar Cells
Authors: Farva Umme , Lee Hyeong Woo , Kim Ri-Na , Lee Dong-Gun , Kang Dong-Won , Kim Jeha
Published: August 2021

Recently, indium oxide (In2O3) thin films have emerged as a promising electron transport layer (ETL) for perovskite solar cells; however, solution-processed In2O3 ETL suffered from poor morphology, pinholes, and required annealing at high temperatures. This research aims to carry out and prepare pinhole-free, transparent, and highly conductive In2O3 thin films via atomic layer deposition (ALD) seizing efficiently as an ETL. In order to explore the growth-temperature-dependent properties of In2O3 thin film, it was fabricated by ALD using the triethyl indium (Et3In) precursor. The detail of the ALD process at 115–250 °C was studied through the film growth rate, crystal structure, morphology, composition, and optical and electrical properties. The film growth rate increased from 0.009 nm/cycle to 0.088 nm/cycle as the growth temperature rose from 115 °C to 250 °C. The film thickness was highly uniform, and the surface roughness was below 1.6 nm. Our results confirmed that film’s structural, optical and electrical properties directly depend on film growth temperature. Film grown at ≥200 °C exhibited a polycrystalline cubic structure with almost negligible carbon impurities. Finally, the device ALD-In2O3 film deposited at 250 °C exhibited a power conversion efficiency of 10.97% superior to other conditions and general SnO2 ETL.
Editorial: Special Issue “Laser-Generated Periodic Nanostructures”
Authors: Simon Peter , Ihlemann Jürgen , Bonse Jörn
Published: August 2021

The study of laser-fabricated periodic nanostructures is one of the leading topics of today’s photonics research [...]
Approaching Disordered Quantum Dot Systems by Complex Networks with Spatial and Physical-Based Constraints
Authors: Cuadra Lucas , Nieto-Borge José Carlos
Published: August 2021

This paper focuses on modeling a disordered system of quantum dots (QDs) by using complex networks with spatial and physical-based constraints. The first constraint is that, although QDs (=nodes) are randomly distributed in a metric space, they have to fulfill the condition that there is a minimum inter-dot distance that cannot be violated (to minimize electron localization). The second constraint arises from our process of weighted link formation, which is consistent with the laws of quantum physics and statistics: it not only takes into account the overlap integrals but also Boltzmann factors to include the fact that an electron can hop from one QD to another with a different energy level. Boltzmann factors and coherence naturally arise from the Lindblad master equation. The weighted adjacency matrix leads to a Laplacian matrix and a time evolution operator that allows the computation of the electron probability distribution and quantum transport efficiency. The results suggest that there is an optimal inter-dot distance that helps reduce electron localization in QD clusters and make the wave function better extended. As a potential application, we provide recommendations for improving QD intermediate-band solar cells.
Keep S’Myelin: World Brain Day 2021 Editorial for Residents and Junior Doctors Page—Neurology International MDPI
Authors: Gunasekera Lakshini , Wijeratne Tissa
Published: August 2021

Every five minutes someone in the world is diagnosed with multiple sclerosis [...]
Malnutrition in Older Adults—Recent Advances and Remaining Challenges
Authors: Norman Kristina , Haß Ulrike , Pirlich Matthias
Published: August 2021

Malnutrition in older adults has been recognised as a challenging health concern associated with not only increased mortality and morbidity, but also with physical decline, which has wide ranging acute implications for activities of daily living and quality of life in general. Malnutrition is common and may also contribute to the development of the geriatric syndromes in older adults. Malnutrition in the old is reflected by either involuntary weight loss or low body mass index, but hidden deficiencies such as micronutrient deficiencies are more difficult to assess and therefore frequently overlooked in the community-dwelling old. In developed countries, the most cited cause of malnutrition is disease, as both acute and chronic disorders have the potential to result in or aggravate malnutrition. Therefore, as higher age is one risk factor for developing disease, older adults have the highest risk of being at nutritional risk or becoming malnourished. However, the aetiology of malnutrition is complex and multifactorial, and the development of malnutrition in the old is most likely also facilitated by ageing processes. This comprehensive narrative review summarizes current evidence on the prevalence and determinants of malnutrition in old adults spanning from age-related changes to disease-associated risk factors, and outlines remaining challenges in the understanding, identification as well as treatment of malnutrition, which in some cases may include targeted supplementation of macro- and/or micronutrients, when diet alone is not sufficient to meet age-specific requirements.
Nutritional Support Indications in Gastroesophageal Cancer Patients: From Perioperative to Palliative Systemic Therapy. A Comprehensive Review of the Last Decade
Authors: Mulazzani Giulia , Corti Francesca , Della Valle Serena , Di Bartolomeo Maria
Published: August 2021

Gastric cancer treatments are rapidly evolving, leading to significant survival benefit. Recent evidence provided by clinical trials strongly encouraged the use of perioperative chemotherapy as standard treatment for the localized disease, whereas in the advanced disease setting, molecular characterization has improved patients’ selection for tailored therapeutic approaches, including molecular targeted therapy and immunotherapy. The role of nutritional therapy is widely recognized, with oncologic treatment’s tolerance and response being better in well-nourished patients. In this review, literature data on strategies or nutritional interventions will be critically examined, with particular regard to different treatment phases (perioperative, metastatic, and palliative settings), with the aim to draw practical indications for an adequate nutritional support of gastric cancer patients and provide an insight on future directions in nutritional strategies. We extensively analyzed the last 10 years of literature, in order to provide evidence that may fit current clinical practice both in terms of nutritional interventions and oncological treatment. Overall, 137 works were selected: 34 Randomized Clinical Trials (RCTs), 12 meta-analysis, 9 reviews, and the most relevant prospective, retrospective and cross-sectional studies in this setting. Eleven ongoing trials have been selected from clinicaltrial.gov as representative of current research. One limitation of our work lies in the heterogeneity of the described studies, in terms of sample size, study procedures, and both nutritional and clinical outcomes. Indeed, to date, there are no specific evidence-based guidelines in this fields, therefore we proposed a clinical algorithm with the aim to indicate an appropriate nutritional strategy for gastric cancer patients.
Neither Beetroot Juice Supplementation nor Increased Carbohydrate Oxidation Enhance Economy of Prolonged Exercise in Elite Race Walkers
Authors: Burke Louise M. , Hall Rebecca , Heikura Ida A. , Ross Megan L. , Tee Nicolin , Kent Georgina L. , Whitfield Jamie , Forbes Sara F. , Sharma Avish P. , Jones Andrew M. , Peeling Peter , Blackwell Jamie R. , Mujika Iñigo , Mackay Karen , Kozior Marta , Vallance Brent , McKay Alannah K. A.
Published: August 2021

Given the importance of exercise economy to endurance performance, we implemented two strategies purported to reduce the oxygen cost of exercise within a 4 week training camp in 21 elite male race walkers. Fourteen athletes undertook a crossover investigation with beetroot juice (BRJ) or placebo (PLA) [2 d preload, 2 h pre-exercise + 35 min during exercise] during a 26 km race walking at speeds simulating competitive events. Separately, 19 athletes undertook a parallel group investigation of a multi-pronged strategy (MAX; n = 9) involving chronic (2 w high carbohydrate [CHO] diet + gut training) and acute (CHO loading + 90 g/h CHO during exercise) strategies to promote endogenous and exogenous CHO availability, compared with strategies reflecting lower ranges of current guidelines (CON; n = 10). There were no differences between BRJ and PLA trials for rates of CHO (p = 0.203) or fat (p = 0.818) oxidation or oxygen consumption (p = 0.090). Compared with CON, MAX was associated with higher rates of CHO oxidation during exercise, with increased exogenous CHO use (CON; peak = ~0.45 g/min; MAX: peak = ~1.45 g/min, p < 0.001). High rates of exogenous CHO use were achieved prior to gut training, without further improvement, suggesting that elite athletes already optimise intestinal CHO absorption via habitual practices. No differences in exercise economy were detected despite small differences in substrate use. Future studies should investigate the impact of these strategies on sub-elite athletes’ economy as well as the performance effects in elite groups.
Decreased Fatty Acid Transporter FABP1 and Increased Isoprostanes and Neuroprostanes in the Human Term Placenta: Implications for Inflammation and Birth Weight in Maternal Pre-Gestational Obesity
Authors: Belcastro Livia , Ferreira Carolina S. , Saraiva Marcelle A. , Mucci Daniela B. , Murgia Antonio , Lai Carla , Vigor Claire , Oger Camille , Galano Jean-Marie , Pinto Gabriela D. A. , Griffin Julian L. , Torres Alexandre G. , Durand Thierry , Burton Graham J. , Sardinha Fátima L. C. , El-Bacha Tatiana
Published: August 2021

The rise in prevalence of obesity in women of reproductive age in developed and developing countries might propagate intergenerational cycles of detrimental effects on metabolic health. Placental lipid metabolism is disrupted by maternal obesity, which possibly affects the life-long health of the offspring. Here, we investigated placental lipid metabolism in women with pre-gestational obesity as a sole pregnancy complication and compared it to placental responses of lean women. Open profile and targeted lipidomics were used to assess placental lipids and oxidised products of docosahexaenoic (DHA) and arachidonic acid (AA), respectively, neuroprostanes and isoprostanes. Despite no overall signs of lipid accumulation, DHA and AA levels in placentas from obese women were, respectively, 2.2 and 2.5 times higher than those from lean women. Additionally, a 2-fold increase in DHA-derived neuroprostanes and a 1.7-fold increase in AA-derived isoprostanes were seen in the obese group. These changes correlated with a 70% decrease in placental FABP1 protein. Multivariate analyses suggested that neuroprostanes and isoprostanes are associated with maternal and placental inflammation and with birth weight. These results might shed light on the molecular mechanisms associated with altered placental fatty acid metabolism in maternal pre-gestational obesity, placing these oxidised fatty acids as novel mediators of placental function.
Chirality-Dependent Anti-Inflammatory Effect of Glutathione after Spinal Cord Injury in an Animal Model
Authors: Kim Seong-Jun , Ko Wan-Kyu , Han Gong-Ho , Lee Daye , Lee Yuhan , Sheen Seung-Hun , Hong Je-Beom , Sohn Seil
Published: August 2021

Neuroinflammation forms a glial scar following a spinal cord injury (SCI). The injured axon cannot regenerate across the scar, suggesting permanent paraplegia. Molecular chirality can show an entirely different bio-function by means of chiral-specific interaction. In this study, we report that d-chiral glutathione (D-GSH) suppresses the inflammatory response after SCI and leads to axon regeneration of the injured spinal cord to a greater extent than l-chiral glutathione (L-GSH). After SCI, axon regrowth in D-GSH-treated rats was significantly increased compared with that in L-GSH-treated rats (*** p < 0.001). Secondary damage and motor function were significantly improved in D-GSH-treated rats compared with those outcomes in L-GSH-treated rats (** p < 0.01). Moreover, D-GSH significantly decreased pro-inflammatory cytokines and glial fibrillary acidic protein (GFAP) via inhibition of the mitogen-activated protein kinase (MAPK) signaling pathway compared with L-GSH (*** p < 0.001). In primary cultured macrophages, we found that D-GSH undergoes more intracellular interaction with activated macrophages than L-GSH (*** p < 0.001). These findings reveal a potential new regenerative function of chiral GSH in SCI and suggest that chiral GSH has therapeutic potential as a treatment of other diseases.