bluuebunny/crossref_metadata_2025 huggingface dataset:
Exploring the use of the Psychological Characteristics of Developing Excellence (PCDEs) in younger age groups: First steps in the validation process of the PCDE Questionnaire for Children (PCDEQ-C)
Authors: Laureys Felien , Collins Dave , Deconinck Frederik J. A. , Lenoir Matthieu
Published: November 2021

Athletes who want to benefit most optimally and efficient from the Talent Development (TD) pathway, should start developing their psychological characteristics at a young age. The Psychological Characteristics Developing Excellence Questionnaire–Version 2 (PCDEQ2) can provide a full assessment of the mental characteristics athletes need. However, the PCDEQ2 has only been validated in adolescent athletes and as a consequence its does not contribute to the understanding of these psychological skills in a younger age group. The main purpose of this study was to take a first step in examining the factor structure and reliability of the Psychological Characteristics of Developing Excellence Questionnaire for Children (PCDEQ-C), a questionnaire based on the PCDEQ– 2. Firstly, the original questionnaire was translated to Dutch, age-adapted and redesigned for implementation in young athletes. Secondly, 774 participants (400 girls) from Flanders, Belgium, between 7 and 13 years (mean age of 10.61 ± 1.58) old filled out the questionnaire. After exploratory factor analysis, a new factor structure for the PCDEQ-C deemed an acceptable fit with 51 items in 5 factors. In the third stage, the reliability showed a good overall and internal consistency, with adequate relations between factors. The first steps in the validation process of the PCDEQ-C, suggest that this questionnaire could be a useful and reliable tool to assess the developmental psychological characteristics of 7-to-13-year-old athletes. The questionnaire is one of the first formative assessment tools to monitor and develop the psychological characteristics needed during the earliest talent development stages of a young athlete.
Line strain representation and shear strain representation of 3D strain states
Authors: Li Shunqun , Cheng Xuelei , Fu Jianbao , Pan Lin , Hai Ran
Published: November 2021

The strain state in 3D space is usually expressed by the conventional method of combining three linear and shear strains. Due to the obvious differences between the first two strains, it is necessary to uncover their properties when describing deformation, studying yield and failure, and developing test apparatus or equipment. The difficulties encountered in the above work would be greatly simplified if strain states could be expressed in a single strain form, namely including only linear or shear strains. As a start, this paper explores the meaning and nature of strain states. Then, based on the hypothesis of small deformations, two strain state expressions, the linear strain expression method (LSEM) and shear strain expression method (SSEM), were established for incompressible materials with only linear strain and shear strain as parameters respectively. Furthermore, conditions, implementation steps and specific forms for the application of SSEM in 1D, 2D and 3D strain states are obtained. As an example, two representations based on tetragonal pyramid and rotating tetrahedron are especially given. Therefore, conventional strain representation methods can be expressed as a combination of line strains in a certain direction or a combination of characteristic shear strains. The results of this paper provide a new way for understanding deformation characteristics, revealing yielding process, establishing constitutive models, and developing testing apparatus or equipment.
Personal relative deprivation and pro-environmental intentions
Authors: Skylark William J. , Callan Mitchell J.
Published: November 2021

Personal relative deprivation (PRD; the belief that one is worse off than other people who are similar to oneself) is associated with a reduced willingness to delay gratification, lower prosociality, and increased materialism. These results suggest that PRD may play a role in shaping people’s willingness to act to protect the natural environment. We report 3 studies that investigate a possible link between PRD and pro-environmental intentions (ENV). Study 1 was an exploratory study using a US sample; Studies 2 and 3 were pre-registered replications using UK and US samples, respectively. In each study, participants self-reported PRD and ENV; they also indicated their subjective social status (where they come on a national “ladder” of social class) and reported their income, education, age, and gender/sex. All three studies found a negative correlation between PRD and ENV. However, multiple regression analyses in which ENV was regressed on PRD and all other variables simultaneously indicated that the unique effect of PRD was small and, for Studies 2 and 3, the 95% confidence intervals included zero. No other variable emerged as a clear unique predictor across all three studies. The data suggest that PRD may be associated with reduced intention to act pro-environmentally, but the causal status of this association, and its relationship to other demographic and social-status variables, remains a topic for further research.
Spatial and temporal characteristics of drought and its correlation with climate indices in Northeast China
Authors: Yue Yuan , Liu HaiFeng , Mu XiuXiang , Qin MengSheng , Wang TingTing , Wang Qi , Yan YaQiong
Published: November 2021

The spatial and temporal characteristics of drought in Northeast China are investigated, using monthly meteorological data from 140 stations over the period 1970–2014. The study area was divided into three regions using hierarchical cluster analysis based on the precipitation and potential evapotranspiration data. The standardized precipitation evapotranspiration index (SPEI) was calculated for each station on 3-month and 12-month time scales. The Mann-Kendall (MK) trend test and Sen’s slope method were applied to determine the trends for annual and seasonal SPEI time series. Periodic features of drought conditions in each sub-region and possible relationship with large-scale climate patterns were respectively identified using the continuous wavelet transform (CWT) and cross wavelet transform. The results show mitigations in spring and winter droughts and a significant increasing trend in autumn drought. On the annual scale, droughts became more severe and more intense in the western regions but were mitigated in the eastern region. CWT analysis showed that droughts in Northeast China occur predominantly in 14- to 42-month or 15- to 60-month cycles. Annual and seasonal droughts have 2- to 6-year cycles over the three defined regions. Cross wavelet analysis also shows that the statistically significant influence of large-scale climate patterns (the Southern Oscillation, the Atlantic Multidecadal Oscillation, the Arctic Oscillation, and the Polar–Eurasian Pattern) on drought in Northeast China is concentrated in a 16- to 50-month period, possibly causing drought variability in the different regions. The Southern Oscillation, Polar–Eurasia pattern, and Arctic Oscillation are significantly correlated with drought on decadal scales (around 120-month period). The findings of this study will provide valuable reference for regional drought mitigation and drought prediction.
Boosting BCG with recombinant influenza A virus tuberculosis vaccines increases pulmonary T cell responses but not protection against Mycobacterium tuberculosis infection
Authors: Muflihah Heni , Flórido Manuela , Lin Leon C. W. , Xia Yingju , Triccas James A. , Stambas John , Britton Warwick J.
Published: November 2021

The current Mycobacterium bovis BCG vaccine provides inconsistent protection against pulmonary infection with Mycobacterium tuberculosis. Immunity induced by subcutaneous immunization with BCG wanes and does not promote early recruitment of T cell to the lungs after M. tuberculosis infection. Delivery of Tuberculosis (TB) vaccines to the lungs may increase and prolong immunity at the primary site of M. tuberculosis infection. Pulmonary immunization with recombinant influenza A viruses (rIAVs) expressing an immune-dominant M. tuberculosis CD4+ T cell epitope (PR8-p25 and X31-p25) stimulates protective immunity against lung TB infection. Here, we investigated the potential use of rIAVs to improve the efficacy of BCG using simultaneous immunization (SIM) and prime-boost strategies. SIM with parenteral BCG and intranasal PR8-p25 resulted in equivalent protection to BCG alone against early, acute and chronic M. tuberculosis infection. Boosting BCG with rIAVs increased the frequency of IFN-γ-secreting specific T cells (p<0.001) and polyfunctional CD4+ T cells (p<0.05) in the lungs compared to the BCG alone, however, this did not result in a significant increase in protection against M. tuberculosis compared to BCG alone. Therefore, sequential pulmonary immunization with these rIAVs after BCG increased M. tuberculosis-specific memory T cell responses in the lung, but not protection against M. tuberculosis infection.
Resource competition and technological diversity
Authors: Mustafin Almaz , Kantarbayeva Aliya
Published: November 2021

The work develops and investigates a mathematical model for evolution of the technological structure of an economic system where different technologies compete for the common essential resources. The model is represented by a system of consumer–resource rate equations. Consumers are technologies formalized as populations of weakly differentiated firms producing a similar commodity with like average output. Firms are characterized by the Leontief–Liebig production function in stock-flow representation. Firms self-replicate with a rate proportional to production output of the respective technology and dissolve with a constant rate of decay. The resources are supplied to the system from outside and consumed by concerned technologies; the unutilized resource amounts are removed elsewhere. The inverse of a per firm break-even resource availability is proposed to serve as a measure for competitiveness towards a given resource. The necessary conditions for coexistence of different technologies are derived, according to which each contender must be a superior competitor for one specific resource and an inferior competitor for the others. The model yields a version of the principle of competitive exclusion: in a steady state, the number of competing technologies cannot exceed the number of limiting resources. Competitive outcomes (either dominance or coexistence) in the general system of multiple technologies feeding on multiple essential resources are shown to be predictable from knowledge of the resource-dependent consumption and growth rates of each technological population taken alone. The proposed model of exploitative competition with explicit resource dynamics enables more profound insight into the patterns of technological change as opposed to conventional mainstream models of innovation diffusion.
Analysing microbiome intervention design studies: Comparison of alternative multivariate statistical methods
Authors: Khomich Maryia , Måge Ingrid , Rud Ida , Berget Ingunn
Published: November 2021

The diet plays a major role in shaping gut microbiome composition and function in both humans and animals, and dietary intervention trials are often used to investigate and understand these effects. A plethora of statistical methods for analysing the differential abundance of microbial taxa exists, and new methods are constantly being developed, but there is a lack of benchmarking studies and clear consensus on the best multivariate statistical practices. This makes it hard for a biologist to decide which method to use. We compared the outcomes of generic multivariate ANOVA (ASCA and FFMANOVA) against statistical methods commonly used for community analyses (PERMANOVA and SIMPER) and methods designed for analysis of count data from high-throughput sequencing experiments (ALDEx2, ANCOM and DESeq2). The comparison is based on both simulated data and five published dietary intervention trials representing different subjects and study designs. We found that the methods testing differences at the community level were in agreement regarding both effect size and statistical significance. However, the methods that provided ranking and identification of differentially abundant operational taxonomic units (OTUs) gave incongruent results, implying that the choice of method is likely to influence the biological interpretations. The generic multivariate ANOVA tools have the flexibility needed for analysing multifactorial experiments and provide outputs at both the community and OTU levels; good performance in the simulation studies suggests that these statistical tools are also suitable for microbiome data sets.
Relationship among state reopening policies, health outcomes and economic recovery through first wave of the COVID-19 pandemic in the U.S.
Authors: Ligo Alexandre K. , Mahoney Emerson , Cegan Jeffrey , Trump Benjamin D. , Jin Andrew S. , Kitsak Maksim , Keenan Jesse , Linkov Igor
Published: November 2021

State governments in the U.S. have been facing difficult decisions involving tradeoffs between economic and health-related outcomes during the COVID-19 pandemic. Despite evidence of the effectiveness of government-mandated restrictions mitigating the spread of contagion, these orders are stigmatized due to undesirable economic consequences. This tradeoff resulted in state governments employing mandates at widely different ways. We compare the different policies states implemented during periods of restriction (“lockdown”) and reopening with indicators of COVID-19 spread and consumer card spending at each state during the first “wave” of the pandemic in the U.S. between March and August 2020. We find that while some states enacted reopening decisions when the incidence rate of COVID-19 was minimal or sustained in its relative decline, other states relaxed socioeconomic restrictions near their highest incidence and prevalence rates experienced so far. Nevertheless, all states experienced similar trends in consumer card spending recovery, which was strongly correlated with reopening policies following the lockdowns and relatively independent from COVID-19 incidence rates at the time. Our findings suggest that consumer card spending patterns can be attributed to government mandates rather than COVID-19 incidence in the states. We estimate the recovery in states that reopened in late April was more than the recovery in states that did not reopen in the same period– 15% for consumer card spending and 18% for spending by high income households. This result highlights the important role of state policies in minimizing health impacts while promoting economic recovery and helps planning effective interventions in subsequent waves and immunization efforts.
Acceptability and efficacy of vaginal self-sampling for genital infection and bacterial vaginosis: A cross-sectional study
Authors: Camus Claire , Penaranda Guillaume , Khiri Hacène , Camiade Sabine , Molet Lucie , Lebsir Melissa , Plauzolles Anne , Chiche Laurent , Blanc Bernard , Quarello Edwin , Halfon Philippe
Published: November 2021

Background & aim

Screening for genital infection (GI) such as bacterial vaginosis (BV) and yeast infection, for sexually transmitted infection (STI), and for asymptomatic carriage of group B streptococcus (GBS) in pregnant women are common reason for medical appointments. The diagnosis and control of GIs, STIs, and GBS are major issues, for fertility and overall well-being of affected women. Conventional testing is performed using vaginal/cervical classical sampling (VCS); this procedure requires pelvic examination performed by health care professionals which raises concerns among women. Vaginal-self-sampling (VSS), as an alternative to VCS, might capture more women.

The aim was first to show non-inferiority of VSS compared with VCS to screen for GIs, STIs, and GBS; second to determine the feasibility of VSS.

Methods

VSS and VCS from 1027 women were collected by health care professionals and simultaneously carried out on each patient. GIs, STIs, and GBS were systematically screened in both paired VSS and VCS samples. Non-inferiority of VSS compared with VCS was assessed using z statistic for binomial proportions.

Results

Prevalence of GIs were 39.7% using VSS and 38.1% using VCS (p = 0.0016). Prevalence of STIs was 8.5% (VSS) vs 8.1% (VCS) (p = 0.0087). Prevalence of GBS was 13.4% (VSS) and 11.5% (VCS) (p = 0.0001). Most participants (84%) recommended the use of VSS.

Conclusions

This study shows that VSS was not inferior to VCS for the detection of GIs, STIs, and GBS. This study provides evidence that VSS can be used as a universal specimen for detection of lower genital tract infections in women.

Study identification number

ID-RCB 2014-A01250-4.
The impact of pandemic-related worry on cognitive functioning and risk-taking
Authors: da Silva Castanheira Kevin , Sharp Madeleine , Otto A. Ross
Published: November 2021

Here, we sought to quantify the effects of experienced fear and worry, engendered by the COVID-19 pandemic, on both cognitive abilities—speed of information processing, task-set shifting, and proactive control—as well as economic risk-taking. Leveraging a repeated-measures cross-sectional design, we examined the performance of 1517 participants, collected during the early phase of the pandemic in the US (April–June 2020), finding that self-reported pandemic-related worry predicted deficits in information processing speed and maintenance of goal-related contextual information. In a classic economic risk-taking task, we observed that worried individuals’ choices were more sensitive to the described outcome probabilities of risky actions. Overall, these results elucidate the cognitive consequences of a large-scale, unpredictable, and uncontrollable stressor, which may in turn play an important role in individuals’ understanding of, and adherence to safety directives both in the current crisis and future public health emergencies.
Diagnosis of bone metastases in breast cancer: Lesion-based sensitivity of dual-time-point FDG-PET/CT compared to low-dose CT and bone scintigraphy
Authors: Hansen Jeanette Ansholm , Naghavi-Behzad Mohammad , Gerke Oke , Baun Christina , Falch Kirsten , Duvnjak Sandra , Alavi Abass , Høilund-Carlsen Poul Flemming , Hildebrandt Malene Grubbe
Published: November 2021

We compared lesion-based sensitivity of dual-time-point FDG-PET/CT, bone scintigraphy (BS), and low-dose CT (LDCT) for detection of various types of bone metastases in patients with metastatic breast cancer. Prospectively, we included 18 patients with recurrent breast cancer who underwent dual-time-point FDG-PET/CT with LDCT and BS within a median time interval of three days. A total of 488 bone lesions were detected on any of the modalities and were categorized by the LDCT into osteolytic, osteosclerotic, mixed morphologic, and CT-negative lesions. Lesion-based sensitivity was 98.2% (95.4–99.3) and 98.8% (96.8–99.5) for early and delayed FDG-PET/CT, respectively, compared with 79.9% (51.1–93.8) for LDCT, 76.0% (36.3–94.6) for BS, and 98.6% (95.4–99.6) for the combined BS+LDCT. BS detected only 51.2% of osteolytic lesions which was significantly lower than other metastatic types. SUVs were significantly higher for all lesion types on delayed scans than on early scans (P<0.0001). Osteolytic and mixed-type lesions had higher SUVs than osteosclerotic and CT-negative metastases at both time-points. FDG-PET/CT had significantly higher lesion-based sensitivity than LDCT and BS, while a combination of the two yielded sensitivity comparable to that of FDG-PET/CT. Therefore, FDG-PET/CT could be considered as a sensitive one-stop-shop in case of clinical suspicion of bone metastases in breast cancer patients.
Effects of salt stress on the photosynthetic physiology and mineral ion absorption and distribution in white willow (Salix alba L.)
Authors: Ran Xin , Wang Xiao , Gao Xiaokuan , Liang Haiyong , Liu Bingxiang , Huang Xiaoxi
Published: November 2021

Objective

The purpose of this study was to explore the adaptive mechanism underlying the photosynthetic characteristics and the ion absorption and distribution of white willow (Salix alba L.) in a salt stress environment in cutting seedlings. The results lay a foundation for further understanding the distribution of sodium chloride and its effect on the photosynthetic system.

Method

A salt stress environment was simulated in a hydroponics system with different NaCl concentrations in one-year-old Salix alba L.branches as the test materials. Their growth, ion absorption, transport and distribution in the roots and leaves, and the changes in the photosynthetic fluorescence parameters were studied after 20 days under hydroponics.

Results

The results show that The germination and elongation of roots are promoted in the presence of 171mM NaCl, but root growth is comprehensively inhibited under increasing salt stress. Under salt stress, Na+ accumulates significantly in the roots and leaves, and the Na+ content and the Na+/K+ and Na+/Ca2+ root ratios are significantly greater than those in the leaves. When the NaCl concentration is ≤ 342mM, Salix alba can maintain relatively stable K+ and Ca2+ contents in its leaves by improving the selective absorption and accumulation of K+ and Ca2+ and adjusting the transport capacity of mineral ions to aboveground parts, while K+ and Ca2+ levels are clearly decreased under high salt stress. With increasing salt concentrations, the net photosynthetic rate (Pn), transpiration rate (E) and stomatal conductance (gs) of leaves decrease gradually overall, and the intercellular CO2 concentration (Ci) first decreases and then increases. When the NaCl concentration is < 342mM, the decrease in leaf Pn is primarily restricted by the stomata. When the NaCl concentration is > 342mM, the decrease in the Pn is largely inhibited by non-stomatal factors. Due to the salt stress environment, the OJIP curve (Rapid chlorophyll fluorescence) of Salix alba turns into an OKJIP curve. When the NaCl concentration is > 171mM, the fluorescence values of points I and P decrease significantly, which is accompanied by a clear inflection point (K). The quantum yield and energy distribution ratio of the PSⅡ reaction center change significantly (φPo, Ψo and φEo show an overall downward trend while φDo is promoted). The performance index and driving force (PIABS, PICSm and DFCSm) decrease significantly when the NaCl concentration is > 171mM, indicating that salt stress causes a partial inactivation of the PSII reaction center, and the functions of the donor side and the recipient side are damaged.

Conclusion

The above results indicate that Salix alba can respond to salt stress by intercepting Na+ in the roots, improving the selective absorption of K+ and Ca2+ and the transport capacity to the above ground parts of the plant, and increasing φDo, thus shows an ability to self-regulate and adapt.
Impact of pre-operative breast magnetic resonance imaging on contralateral synchronous and metachronous breast cancer detection—A case control comparison study with 1468 primary operable breast cancer patients with mean follow-up of 102 months
Authors: Wu Wen-Pei , Chen Chih-Yu , Lee Chih-Wei , Wu Hwa-Koon , Chen Shou-Tung , Wu Yu-Ting , Lin Ying-Jen , Chen Dar-Ren , Kuo Shou-Jen , Lai Hung-Wen
Published: November 2021

Background

Women with unilateral breast cancer are at an increased risk for the development of contralateral breast cancers. We hypothesis that combined breast MRI would detect more contralateral synchronous breast cancer than conventional imaging alone, and resulted in less contralateral metachronous breast cancer during follow-up.

Methods

We retrospectively collected two groups of breast cancer patients diagnosed from 2009 to 2013 for evaluating the effectiveness and value of adding pre-operative breast MRI to conventional breast images (mammography and sonography) for detection of contralateral synchronous breast cancer. The new metachronous contralateral breast cancer diagnosed during follow-up was prospectively evaluated and compared.

Results

Group A (n = 733) comprised patients who underwent conventional preoperative imaging and group B (n = 735) combined with MRI were enrolled and compared. Seventy (9.5%) of the group B patients were found to have contralateral lesions detected by breast MRI, and 65.7% of these lesions only visible with MRI. The positive predictive value of breast MRI detected contralateral lesions was 48.8%. With the addition of breast MRI to conventional imaging studies, more surgical excisions were performed in contralateral breasts (6% (44/735) versus 1.4% (10/733), P< 0.01), more synchronous contralateral breast cancer detected (2.9% (21/735) versus 1.1% (8/733), P = 0.02), and resulted in numerical less (2.2% (16/714) versus 3% (22/725), p = 0.3) metachronous contralateral breast cancer during a mean follow-up of 102 months.

Conclusions

Our study provides useful estimates of the pre-operative breast MRI for the increased detection of contralateral synchronous breast cancer and less subsequent contralateral metachronous breast cancer.
Risk of hospitalized and non-hospitalized gastrointestinal bleeding in ALLHAT trial participants receiving diuretic, ACE-inhibitor, or calcium-channel blocker
Authors: Du Xianglin L. , Simpson Lara M. , Tandy Brian C. , Bettencourt Judith L. , Davis Barry R.
Published: November 2021

Objectives

This post-trial data linkage analysis was to utilize the data of Antihypertensive and Lipid-Lowering Treatment to Prevent Heart Attack Trial (ALLHAT) participants linked with their Medicare data to examine the risk of hospitalized and non-hospitalized gastrointestinal (GI) bleeding associated with antihypertensives.

Settings

ALLHAT was a multicenter, randomized, double-blind, active-controlled trial conducted in a total of 42,418 participants aged ≥55 years with hypertension in 623 North American centers. Data for ALLHAT participants who were aged at ≥65 have been linked with their Medicare claims data.

Participants

A total of 16,676 patients (4,480 for lisinopril, 4,537 for amlodipine, and 7,659 for chlorthalidone) with complete Medicare claims data were available for the final analysis.

Results

The cumulative incidences through March 31, 2002 of hospitalized GI bleeding were 5.4%, 5.8% and 5.4% for amlodipine, lisinopril, and chlorthalidone arms, respectively, but were not statistically significant among the 3 arms after adjusting for confounders in Cox regression models. The cumulative incidences of non-hospitalized GI bleeding were also similar across the 3 arms (12.0%, 12.2% and 12.0% for amlodipine, lisinopril, and chlorthalidone, respectively). The increased risk of GI bleeding by age was statistically significant after adjusting for confounders (HR = 1.04 per year, 95% CI: 1.03–1.05). Smokers also had a significantly higher risk of having hospitalized GI bleeding (1.45, 1.19–1.76). Hispanics, those who used aspirin or atenolol in-trial, had diabetes, more education, and a history of stroke had a significantly lower risk of having GI bleeding than their counterparts. Other factors such as gender, history of CHD, prior antihypertensive use, use of estrogen in women, and obesity did not have significant effects on the risk of GI bleeding.

Conclusion

There were no statistically significant differences on the risk of hospitalized or non-hospitalized GI bleeding among the 3 ALLHAT trial arms (amlodipine, lisinopril, and chlorthalidone) during the entire in-trial follow-up.
The effect of soil sample size, for practical DNA extraction, on soil microbial diversity in different taxonomic ranks
Authors: Morita Hiroki , Akao Satoshi
Published: November 2021

To determine the optimal soil sample size for microbial community structure analysis, DNA extraction, microbial composition analysis, and diversity assessments were performed using soil sample sizes of 0.2, 1, and 5 g. This study focused on the relationship between soil amount and DNA extraction container volume and the alteration in microbial composition at different taxonomic ranks (order, class, and phylum). Horizontal (0.2 and 1 g) and vertical (5 g) shaking were applied during DNA extraction for practical use in a small laboratory. In the case of the 5 g soil sample, DNA extraction efficiency and the value of α-diversity index fluctuated severely, possibly because of vertical shaking. Regarding the 0.2 and 1 g soil samples, the number of taxa, Shannon–Wiener index, and Bray–Curtis dissimilarity were stable and had approximately the same values at each taxonomic rank. However, non-metric multidimensional scaling showed that the microbial compositions of these two sample sizes were different. The higher relative abundance of taxa in the case of the 0.2 g soil sample might indicate that cell wall compositions differentiated the microbial community structures in these two sample sizes due to high shear stress tolerance. The soil sample size and tube volume affected the estimated microbial community structure. A soil sample size of 0.2 g would be preferable to the other sample sizes because of the possible higher shearing force for DNA extraction and lower experimental costs due to smaller amounts of consumables. When the taxonomic rank was changed from order to phylum, some minor taxa identified at the order rank were integrated into major taxa at the phylum rank. The integration affected the value of the β-diversity index; therefore, the microbial community structure analysis, reproducibility of structures, diversity assessment, and detection of minor taxa would be influenced by the taxonomic rank applied.
Determining the timing of pubertal onset via a multicohort analysis of growth
Authors: Syrjälä Essi , Niinikoski Harri , Virtanen Helena E. , Ilonen Jorma , Knip Mikael , Hutri-Kähönen Nina , Pahkala Katja , Raitakari Olli T. , Rodprasert Wiwat , Toppari Jorma , Virtanen Suvi M. , Veijola Riitta , Peltonen Jaakko , Nevalainen Jaakko
Published: November 2021

Objective

Growth-based determination of pubertal onset timing would be cheap and practical. We aimed to determine this timing based on pubertal growth markers. Secondary aims were to estimate the differences in growth between cohorts and identify the role of overweight in onset timing.

Design

This multicohort study includes data from three Finnish cohorts—the Type 1 Diabetes Prediction and Prevention (DIPP, N = 2,825) Study, the Special Turku Coronary Risk Factor Intervention Project (STRIP, N = 711), and the Boy cohort (N = 66). Children were monitored for growth and Tanner staging (except in DIPP).

Methods

The growth data were analyzed using a Super-Imposition by Translation And Rotation growth curve model, and pubertal onset analyses were run using a time-to-pubertal onset model.

Results

The time-to-pubertal onset model used age at peak height velocity (aPHV), peak height velocity (PHV), and overweight status as covariates, with interaction between aPHV and overweight status for girls, and succeeded in determining the onset timing. Cross-validation showed a good agreement (71.0% for girls, 77.0% for boys) between the observed and predicted onset timings. Children in STRIP were taller overall (girls: 1.7 [95% CI: 0.9, 2.5] cm, boys: 1.0 [0.3, 2.2] cm) and had higher PHV values (girls: 0.13 [0.02, 0.25] cm/year, boys: 0.35 [0.21, 0.49] cm/year) than those in DIPP. Boys in the Boy cohort were taller (2.3 [0.3, 4.2] cm) compared with DIPP. Overweight girls showed pubertal onset at 1.0 [0.7, 1.4] year earlier compared with other girls. In boys, there was no such difference.

Conclusions

The novel modeling approach provides an opportunity to evaluate the Tanner breast/genital stage–based pubertal onset timing in cohort studies including longitudinal data on growth but lacking pubertal follow-up.
A dataset for the study of identity at scale: Annual Prevalence of American Twitter Users with specified Token in their Profile Bio 2015–2020
Authors: Jones Jason Jeffrey
Published: November 2021

Personally expressed identity is who or what an individual themselves says they are, and it should be studied at scale. At scale means with data on millions of individuals, which is newly available and comes timestamped and geocoded. This work introduces a dataset for the study of identity at scale and describes the method for collecting and aggregating such data. Further, tools and theory for working with the data are presented. A demonstration analysis provides evidence that personal, individual development and changing cultural norms can be observed with these data and methods.
S100A8 expression in oviduct mucosal epithelial cells is regulated by estrogen and affects mucosal immune homeostasis
Authors: Li Xiaodan , Cao Guifang , Yang Hongxin , Zhi Dafu , Li Lei , Wang Daqing , Liu Moning , Su Hong
Published: November 2021

Chronic inflammation can cause oviduct mucosal damage and immune dysfunction, leading to infertility, early pregnancy loss, ectopic pregnancy, tumors, and a decrease in reproductive capacities in female animals. Estrogen can suppress immune responses in different tissues and oviducts, and regulate the oviduct immune balance; however, the underlying mechanisms remain unclear. The objective of this study was to explore the mechanism of estrogen-regulated oviduct mucosal immunity and discover new estrogen targets for regulating oviduct mucosal immune homeostasis. Sheep oviduct epithelial cells (SOECs) were treated with 17-β estradiol (E2). Transcriptome sequencing and analysis showed differentially expressed S100 calcium-binding protein A (S100A) genes that may participate in the oviduct mucosa immunoregulation of estrogen. Quantitative polymerase chain reaction and immunocytochemistry analysis showed thatS100A8expression changed dynamically in E2-treated SOECs and peaked after 7 h of treatment. Estrogen nuclear receptors and G protein-coupled membrane receptors promoted E2-dependentS100A8upregulation. TheS100A8gene was disrupted using the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 method. Levels of inflammatory factors interleukin (IL)-1β and IL-4 were significantly upregulated inS100A8-knockdown SOECs, whereas those of the anti-inflammatory factor IL-10 was downregulated. Following S100A8 knockdown in SOECs treated with E2 for 7 h, IL-10 levels increased significantly. Estrogen affected oviduct mucosa immune function and dynamically regulated S100A8 in SOECs. S100A8 knockdown caused an excessive immune response, indicating that S100A8 is beneficial for maintaining immune homeostasis in the oviduct mucosa. Moreover, estrogen can compensate for the effect of S100A8 knockdown by upregulating IL-10.
Association between inflammatory cytokines and immune–checkpoint molecule in rheumatoid arthritis
Authors: Matsumoto Haruki , Fujita Yuya , Asano Tomoyuki , Matsuoka Naoki , Temmoku Jumpei , Sato Shuzo , Yashiro–Furuya Makiko , Yokose Kohei , Yoshida Shuhei , Suzuki Eiji , Yago Toru , Watanabe Hiroshi , Kawakami Atsushi , Migita Kiyoshi
Published: November 2021

Background

Anti-citrullinated peptide antibodies (ACPA) and inflammatory cytokines play important roles in the development of rheumatoid arthritis (RA). T cell immunoglobulin and mucin–domain containing–3 (TIM–3) is an immune-checkpoint molecule involved in inhibitory signaling. Galectin–9 (Gal–9) mediated ligation of TIM–3 induces the amelioration of autoimmune diseases. TIM–3 is expressed in synovial osteoclasts and involved in the rheumatoid bone destruction. The aim of this study was to investigate the relationships between inflammatory cytokines and immune–checkpoint molecules in RA patients.

Methods

Serum levels of interleukin–6 (IL–6), tumor necrosis factor–α (TNF–α), soluble TIM–3 (sTIM–3) and Gal–9 were determined by ELISA. Patients were stratified into two groups based on ACPA titers: low-medium ACPA (ACPA <200 U/mL) and high ACPA (ACPA ≥200 U/mL). Serum levels of cytokines or immune-checkpoint molecules were evaluated between RA patients with low-medium ACPA titers and high ACPA titers.

Results

Elevated serum levels of inflammatory cytokines were correlated with DAS28–ESR in RA patients. Although serum levels of sTIM–3 were elevated in RA patients, significant correlations between sTIM–3 and cytokines (IL–6 or TNF–α) were observed exclusively in RA patients with low-medium ACPA titers (<200 U/mL). Serum levels of IL–6 and TNF–α levels were significantly correlated with elevated Gal–9 levels regardless of ACPA status. A significant correlation between IL–6 and Gal–9 was observed in RA patients without advanced joint damage. Conversely, a significant correlation between TNF–α and Gal–9 was observed in RA patients with advanced joint damage.

Conclusions

Our data indicated that there are positive correlations between circulating inflammatory cytokines and checkpoint molecules in RA patients and these interactions can be modulated by ACPA status or joint damage stage.
Peptides derived from the SARS-CoV-2 receptor binding motif bind to ACE2 but do not block ACE2-mediated host cell entry or pro-inflammatory cytokine induction
Authors: Mahindra Amit , Tejeda Gonzalo , Rossi Mario , Janha Omar , Herbert Imogen , Morris Caroline , Morgan Danielle C. , Beattie Wendy , Montezano Augusto C. , Hudson Brian , Tobin Andrew B. , Bhella David , Touyz Rhian M. , Jamieson Andrew G. , Baillie George S. , Blair Connor M.
Published: November 2021

SARS-CoV-2 viral attachment and entry into host cells is mediated by a direct interaction between viral spike glycoproteins and membrane bound angiotensin-converting enzyme 2 (ACE2). The receptor binding motif (RBM), located within the S1 subunit of the spike protein, incorporates the majority of known ACE2 contact residues responsible for high affinity binding and associated virulence. Observation of existing crystal structures of the SARS-CoV-2 receptor binding domain (SRBD)–ACE2 interface, combined with peptide array screening, allowed us to define a series of linear native RBM-derived peptides that were selected as potential antiviral decoy sequences with the aim of directly binding ACE2 and attenuating viral cell entry. RBM1 (16mer): S443KVGGNYNYLYRLFRK458, RBM2A (25mer): E484GFNCYFPLQSYGFQPTNGVGYQPY508, RBM2B (20mer): F456NCYFPLQSYGFQPTNGVGY505 and RBM2A-Sc (25mer): NYGLQGSPFGYQETPYPFCNFVQYG. Data from fluorescence polarisation experiments suggested direct binding between RBM peptides and ACE2, with binding affinities ranging from the high nM to low μM range (Kd = 0.207–1.206 μM). However, the RBM peptides demonstrated only modest effects in preventing SRBD internalisation and showed no antiviral activity in a spike protein trimer neutralisation assay. The RBM peptides also failed to suppress S1-protein mediated inflammation in an endogenously expressing ACE2 human cell line. We conclude that linear native RBM-derived peptides are unable to outcompete viral spike protein for binding to ACE2 and therefore represent a suboptimal approach to inhibiting SARS-CoV-2 viral cell entry. These findings reinforce the notion that larger biologics (such as soluble ACE2, ‘miniproteins’, nanobodies and antibodies) are likely better suited as SARS-CoV-2 cell-entry inhibitors than short-sequence linear peptides.
Lessons learned from the rapid development of a statewide simulation model for predicting COVID-19’s impact on healthcare resources and capacity
Authors: Endres-Dighe Stacy , Jones Kasey , Hadley Emily , Preiss Alexander , Kery Caroline , Stoner Marie , Eversole Susan , Rhea Sarah
Published: November 2021

The first case of COVID-19 was detected in North Carolina (NC) on March 3, 2020. By the end of April, the number of confirmed cases had soared to over 10,000. NC health systems faced intense strain to support surging intensive care unit admissions and avert hospital capacity and resource saturation. Forecasting techniques can be used to provide public health decision makers with reliable data needed to better prepare for and respond to public health crises. Hospitalization forecasts in particular play an important role in informing pandemic planning and resource allocation. These forecasts are only relevant, however, when they are accurate, made available quickly, and updated frequently. To support the pressing need for reliable COVID-19 data, RTI adapted a previously developed geospatially explicit healthcare facility network model to predict COVID-19’s impact on healthcare resources and capacity in NC. The model adaptation was an iterative process requiring constant evolution to meet stakeholder needs and inform epidemic progression in NC. Here we describe key steps taken, challenges faced, and lessons learned from adapting and implementing our COVID-19 model and coordinating with university, state, and federal partners to combat the COVID-19 epidemic in NC.
Differential contribution of two organelles of endosymbiotic origin to iron-sulfur cluster synthesis and overall fitness in Toxoplasma
Authors: Pamukcu Sarah , Cerutti Aude , Bordat Yann , Hem Sonia , Rofidal Valérie , Besteiro Sébastien
Published: November 2021

Iron-sulfur (Fe-S) clusters are one of the most ancient and ubiquitous prosthetic groups, and they are required by a variety of proteins involved in important metabolic processes. Apicomplexan parasites have inherited different plastidic and mitochondrial Fe-S clusters biosynthesis pathways through endosymbiosis. We have investigated the relative contributions of these pathways to the fitness ofToxoplasma gondii, an apicomplexan parasite causing disease in humans, by generating specific mutants. Phenotypic analysis and quantitative proteomics allowed us to highlight notable differences in these mutants. Both Fe-S cluster synthesis pathways are necessary for optimal parasite growth in vitro, but their disruption leads to markedly different fates: impairment of the plastidic pathway leads to a loss of the organelle and to parasite death, while disruption of the mitochondrial pathway trigger differentiation into a stress resistance stage. This highlights that otherwise similar biochemical pathways hosted by different sub-cellular compartments can have very different contributions to the biology of the parasites, which is something to consider when exploring novel strategies for therapeutic intervention.
‘When I say “modern”, you say “band”’: A critical narrative of modern band and Little Kids Rock as music education curriculum
Authors: Rathgeber Jesse , Bernard Cara Faith
Published: November 2021

This article is based on the lived experiences of the authors engaging with modern band and the organization Little Kids Rock (LKR). We approach this research as critical storytelling to highlight the importance of critique of music curriculum and pedagogy. We identify moments of cognitive dissonance we experienced with LKR and modern band and unpack them through theory. Data included review of LKR materials, journals, text messages, reflective writing and discussion around participation in LKR-sponsored events. We share our critical story through text messages and narration, through which we note issues such as neo-liberalism and indoctrination; language (mis)use through educational buzzwords; identity reformation and the manner in which teachers feel a need to cling to methodolatry or act as change agents. We illustrate the central role critique plays in music teaching discourses and practices to guide music teachers to accept vigilance of curricular resources and pedagogical approaches presented to them.
LEGAL REGULATION CYBERSPORT OF IN RUSSIA AND SOME ASIAN COUNTRIES
Authors: Kovalenko E.Y. , Tydykova N.V. , A.K. Kusainova
Published: November 2021

The article is devoted to the study of cybersport as a legal phenomenon. The analysis of the Russianlegislation showed that the term “computer sports’ is the most frequently used in the texts of regulations.Equivalent to this term are such as “cybersport” and “e-sports”. Russia became the first country in the worldto recognize cybersport as an official sport. Currently, the legal regulation of cybersport in Russia is carriedout mainly at the subordinate level. The main regulations in this area are listed. The authors came to theconclusion that cybersport relations are an understudied area, so it is still difficult to make a decision onthe need for a special law. It is proved that such a decision will be justified if such a specificity of this sportis established, the level of which will not allow the existing legislation to be applied to these relations andwill require other approaches. The main discussions on the studied issue that take place in the scientificliterature are named. Special legislation on international cybersport has not yet emerged either, althoughthe development of cybersport in the world is quite dynamic, and the International Esports Federation unites 100 countries, and the Asian Electronic Sports Federation — 45 countries. Some features of the developmentand legal regulation of the area under consideration in some Asian countries are identified. The authorspredict further development of legislation in this area in the near future.
ORHON’S MONUMENTS (8-TH CENTURY) AND “THE FAMILY TREE THE TURKMENS” ABU-L-GAZI (17-TH CENTURY)
Authors: M. Soyegov
Published: November 2021

On the basis of the comparative analysis of the corresponding sources concerning to stories theTurkmens and their direct ancestors — Oghuzs, along with an establishment of conceptual approaches toa problem the concrete episodes not revealed by researchers which serve also as a substantiation of theseapproaches are described earlier.
ON PRESSING ISSUES OF CRIMINAL LEGAL PROTECTION OF CREDITORS AT THE STAGE OF EXECUTION OF JUDICIAL ACTS ON THE COLLECTION OF ACCOUNTS PAYABLE
Authors: Sasykin K.Y.
Published: November 2021

In the article, the author examines the problem of criminal legal protection of creditors’ rights fromcrimes associated with malicious evasion by debtors from paying off accounts payable, for the commissionof which liability is provided under Article 177 of the Criminal Code of the Russian Federation. The author emphasizes that the norm of the article was introduced in 1996 together with the adoptionof the code and the disposition did not undergo changes over the next twenty-five years, remaininginsufficiently regulated. It is indicated with reference to judicial practice on the problem of the objective sideof the corpus delicti of this crime, which contains ambiguous evaluative signs.The author, based on the analysis of the article of the considered norm and a number of doctrinal pointsof view, concluded that the norm is subject to change with the “removal” of the controversial terminologyfrom the disposition. The author proposes a revision of the norm and, as an example, provides a similarprovision on liability from the criminal legislation of the Republic of Belarus.Also, based on the analysis of articles 144–145 of the Criminal Procedure Code of the Russian Federationand taking into account the practice and doctrinal points of view, attention is focused on the need, in additionto substantive changes, changes and procedural norms of domestic legislation, namely, on the need totransform the stage existing in the Criminal Procedure Code of the Russian Federation initiation of a criminalcase as hindering the timely detection and investigation of this type of crime.
ON THE METHODS OF CONCLUDING CIVIL LAW TRANSACTIONS WITH THE USE OF ELECTRONIC TECHNICAL MEANS
Authors: Suleimenova A.R.
Published: November 2021

The article discusses issues of concluding contracts using electronic technical means, highlights moderntrends and changes in legislation in this area. The author assumes that transactions using technical meanshave their own distinctive features, which are reflected in their conclusion. The article presents ways toconclude transactions in the electronic space, as well as possible options for overcoming them, which willhelp protect the participants in such a transaction.
STATE OF THE KYRGYZ IN THE EARLY MIDDLE AGES
Authors: Borubashov B.I.
Published: November 2021

The article deals with the evolution of the political and legal system of the Kyrgyz society during theEarly Middle Ages. In the 6th century, the Khagyas (Kyrgyz) State was formed on the Yenisei River on thebasis of the unification of the Altai and Yenisei Kyrgyz tribes. The IX-beginning of the X century — the periodof the greatest growth of the power of the Kyrgyz power was called the “Kyrgyz Great Power”.