bluuebunny/crossref_metadata_2025 huggingface dataset:
Global Solar Irradiation Modelling and Prediction Using Machine Learning Models for Their Potential Use in Renewable Energy Applications
Authors: Puga-Gil David , Astray Gonzalo , Barreiro Enrique , Gálvez Juan F. , Mejuto Juan Carlos
Published: December 2022

Global solar irradiation is an important variable that can be used to determine the suitability of an area to install solar systems; nevertheless, due to the limitations of requiring measurement stations around the entire world, it can be correlated with different meteorological parameters. To confront this issue, different locations in Rias Baixas (Autonomous Community of Galicia, Spain) and combinations of parameters (month and average temperature, among others) were used to develop various machine learning models (random forest -RF-, support vector machine -SVM- and artificial neural network -ANN-). These three approaches were used to model and predict (one month ahead) monthly global solar irradiation using the data from six measurement stations. Afterwards, these models were applied to seven different measurement stations to check if the knowledge acquired could be extrapolated to other locations. In general, the ANN models offered the best results for the development and testing phases of the model, as well as for the phase of knowledge extrapolation to other locations. In this sense, the selected ANNs obtained a mean absolute percentage error (MAPE) value between 3.9 and 13.8% for the model development and an overall MAPE between 4.1 and 12.5% for the other seven locations. ANNs can be a capable tool for modelling and predicting monthly global solar irradiation in areas where data are available and for extrapolating this knowledge to nearby areas.
Go-MoS2/Water Flow over a Shrinking Cylinder with Stefan Blowing, Joule Heating, and Thermal Radiation
Authors: Narayanaswamy Manoj Kumar , Kandasamy Jagan , Sivanandam Sivasankaran
Published: December 2022

The impacts of Stefan blowing along with slip and Joule heating on hybrid nanofluid (HNF) flow past a shrinking cylinder are investigated in the presence of thermal radiation. Using the suitable transformations, the governing equations are converted into ODEs, and the MATLAB tool bvp4c is used to solve the resulting equations. As Stefan blowing increases, temperature and concentration profiles are accelerated but the velocity profile diminishes and also the heat transfer rate improves up to 25% as thermal radiation upsurges. The mass transfer rate diminishes as increasing Stefan blowing. The Sherwood number, the Nusselt number, and the skin friction coefficient are numerically tabulated and graphs are also plotted. The outcomes are conscientiously and thoroughly discussed.
Papillary Squamotransitional Cell Carcinoma of the Uterine Cervix with Atypical Presentation: A Case Report with a Literature Review
Authors: Yordanov Angel , Karaivanov Milen , Kostov Stoyan , Kornovski Yavor , Ivanova Yonka , Slavchev Stanislav , Todorova Venelina , Vasileva-Slaveva Mariela
Published: December 2022

Introduction: Cervical cancer is the fourth most prevalent malignancy and the fourth leading cause of cancer-related death in women around the world. Histologically, squamous cell carcinoma (SCC) is the most common form of cervical cancer. SCC has several subtypes, and one of the rarest is papillary squamotransitional cell carcinoma (PSCC). In general, PSCC is believed to have a similar course and prognosis to typical SCC, with a high risk of late metastasis and recurrence. Case report: We discuss the case of a 45-year-old patient diagnosed with PSCC who was admitted to our department in December 2021. The clinical manifestations were pelvic discomfort and lymphadenopathy throughout the body. On admission, all laboratory values, with the exception of C-Reactive Protein (CRP) at 22.35 mg/L and hemoglobin (HGB) at 87.0 g/L, were normal. The clinical and ultrasound examination revealed a painful formation with indistinct borders in the right portion of the small pelvis. Following dilation and curettage, a Tru-Cut biopsy of the inguinal lymph nodes was performed. The investigation histologically indicated PSCC. MRI of the small pelvis showed an endophytic tumor in the cervix with dimensions of 35/26 mm and provided data for bilateral parametrial infiltration; a hetero-intensive tumor originating from the right ovary and involving small intestinal loops measuring 90/58 mm; and generalized lymphadenopathy and peritoneal metastases in the pouch of Douglass. The FIGO classification for the tumor was IVB. The patient was subsequently referred for chemotherapy by the tumor board’s decision. Discussion: Despite the generally good prognosis of SCC, PSCC is a rare and aggressive subtype. It is usually diagnosed at an advanced stage and has a poor prognosis. Conclusions: PSCC is a rare subtype of SCC, and its diagnosis and treatment are challenging.
Novel Magnetic Mixed Cellulose Acetate Matrix Membranes with Oxygen-Enrichment Potential
Authors: Nady Norhan , Salem Noha , Elmarghany Mohamed R. , Salem Mohamed S. , Kandil Sherif H.
Published: December 2022

This work presents novel magnetic mixed cellulose-based matrix membranes that combine the advantages of a low-cost common polymer matrix, such as cellulose acetate (CA), and a low-cost magnetic filler. Moreover, the presented magnetic mixed CA matrix membranes were fabricated and used without applying an external magnetic field during either the membrane casting or the separating process. Poly(methylmethacrylate) and lithium chloride were used in order to improve the mechanical properties and porosity of the fabricated membranes. The iron–nickel magnetic alloys used were prepared through a simple chemical reduction method with unique morphologies (Fe10Ni90—starfish-like and Fe20Ni80—necklace-like). The novel magnetic mixed CA matrix membranes fabricated were characterized using different analysis techniques, including SEM, EDX, XRD, TGA, and FTIR-ATR analyses. Furthermore, the static water contact angle, membrane thickness, surface roughness, tensile strength, and membrane porosity (using ethanol and water) were determined. In addition, vibrating sample magnetometer (VSM) analysis was conducted and the oxygen transition rate (OTR) was studied. The magnetic mixed CA matrix membrane containing starfish-like Fe10Ni90 alloy was characterized by high coercivity (109 Oe) and an efficient 1.271 × 10−5 cm3/(m2·s) OTR compared to the blank CA membrane with 19.8 Oe coercivity and no OTR. The effects of the polymeric matrix composition, viscosity, and compatibility with the alloys/fillers used on the structure and performance of the fabricated mixed CA matrix membranes compared to the previously used poly(ethersufone) polymeric matrix are discussed and highlighted. The novel magnetic mixed CA matrix membranes presented have good potential for use in the oxygen-enrichment process.
Performance Comparison of Proton Exchange Membrane Water Electrolysis Cell Using Channel and PTL Flow Fields through Three-Dimensional Two-Phase Flow Simulation
Authors: Park Seongsoon , Lee Woojung , Na Youngseung
Published: December 2022

Water electrolysis technology is required to overcome the intermittency of renewable energy sources. Among various water electrolysis methods, the proton exchange membrane water electrolysis (PEMWE) cell has the advantages of a fast response and high current density. However, high capital costs have hindered the commercialization of PEMWE; therefore, it is important to lower the price of bipolar plates, which make PEMWE expensive. In addition, since the flow field inscribed in the bipolar plate significantly influences the performance, it is necessary to design the enhanced pattern. A three-dimensional two-phase flow model was used to analyze the two-phase flow and electrochemical reactions of the PEMWE anode. In order to compare the experimental results with the simulation, experiments were conducted according to the flow rate, and the results were in good agreement. First, as a result of comparing the performance of the channel and PTL (porous transport layer) flow fields, the channel flow field showed better performance than the PTL flow field. For the channel flow field, the higher the ratio of the channel width-to-rib width and the permeability of PTL, the performance got better. In the case of the PTL flow field, with the increased capillary pressure, the performance improved even if the PTL permeability decreased. Next, the direction of gravity affected the performance only when the channel flow field was used, and the X+ and Z+ directions were optimal for the performance. Finally, increasing the inlet flow rate could reduce the difference in performance between the channel and PTL flow fields, but the pressure drop gradually increased.
Magnetocaloric Effect in CoFe-Electroplated Ni50Mn33In16Cr1 Alloy
Authors: Lekkla Peerapat , Jantaratana Pongsakorn , Chotibhawaris Thanakrit
Published: December 2022

A high-saturation magnetization CoFe layer was electroplated onto Ni50Mn33In16Cr1 alloy using a magnetic field assistance electroplating bath. This CoFe-coated alloy can function as an active magnetic regenerator owing to its magnetocaloric effect. The CoFe coating layer did not affect the entropy change calculated from the isothermal magnetization of the alloy, but it significantly affected the temperature variation of the alloy by changing the externally applied magnetic field. The temperature change of the CoFe-coated alloy increases with increasing CoFe coating times. By comparing with the as-sintered alloy, a maximum increase of 150% in temperature change can be observed in the alloy coated with CoFe for 2 h.
First Principles Investigation of the Effects of Chemical Short-Range Ordering Clusters on the Ideal Tensile Strength and Ductility of Aluminum Alloys
Authors: Chen Xiangkai , Chen Xiaohua , Wang Zidong , Wang Yanlin , Chen Kaixuan , Zhu Yuzhi
Published: December 2022

As important structural features of the metal materials, chemical short-range ordering clusters play a critical role in the mechanical properties of the materials. They have been discovered in dilute Al-alloy systems and are usually generated by annealing processes at high temperatures or by severe plastic deformation at room temperature. In the present work, systematic first-principle calculations were conducted to evaluate the influences of the chemical short-range ordering clusters L12-Al3Zr on the mechanical properties of the pure Al supercell. Results showed that the mechanical properties including both ideal tensile strength and ductility were improved simultaneously when the chemical short-range ordering clusters L12-Al3Zr were introduced to the pure Al. The larger the volume fraction of chemical short-range ordering clusters L12-Al3Zr, the larger the ideal tensile strength. The deformation charge density, the electron localization function and the density of state were computed to reveal the nature of the strengthening of the chemical short-range ordering clusters L12-Al3Zr on the pure Al supercell. It was found that excellent ideal tensile strength for the Al supercell with the chemical short-range ordering clusters L12-Al3Zr was due to strong charge accumulations and strong electronic interactions between the solute atoms Zr and the host Al atoms. In addition, the Pugh ratio (B/G) and ratio (Wsep/Gdisl) of the work of the separation Wsep to the work of dislocation emission Gdisl were computed to reveal the effect of the chemical short-range ordering clusters L12-Al3Zr on the ductility of the Al supercell. Results showed that the addition of L12-Al3Zr chemical short-range ordering clusters addition to pure Al supercell brought about an increase in ductility as compared to pure Al supercell, which is ascribed to large the Pugh ratio B/G and ratio (Wsep/Gdisl) of the work of the separation Wsep to the work of dislocation emission Gdisl. This work is important for simultaneously improving the tensile strength and ductility of Al alloys.
Baseline Correction of the Human 1H MRS(I) Spectrum Using T2* Selective Differential Operators in the Frequency Domain
Authors: Choi Sang-Han , Ryu Yeun-Chul , Chung Jun-Young
Published: December 2022

The baseline distortion caused by water and fat signals is a crucial issue in the 1H MRS(I) study of the human brain. This paper suggests an effective and reliable preprocessing technique to calibrate the baseline distortion caused by the water and fat signals exhibited in the MRS spectral signal. For the preprocessing, we designed a T2* (or linewidth within the spectral signal) selective filter for the MRS(I) data based on differential filtering within the frequency domain. The number and types for the differential filtering were determined by comparing the T2* selectivity profile of each differential operator with the T2* profile of the metabolites to be suppressed within the MRS(I) data. In the performance evaluation of the proposed differential filtering, the simulation data for MRS spectral signals were used. Furthermore, the spectral signal of the human 1H MRSI data obtained by 2D free induction decay chemical shift imaging with a typical water suppression technique was also used in the performance evaluation. The absolute values of the average of the filtered dataset were quantitatively analyzed using the LCModel software. With the suggested T2* selective (not frequency selective) filtering technique, in the simulated MRS data, we removed the metabolites from the simulated MRS(I) spectral signal baseline distorted by the water and fat signal observed in the most frequency band. Moreover, in the obtained MRSI data, the quantitative analysis results for the metabolites of interest showed notable improvement in the uncertainty estimation accuracy, the CRLB (Cramer-Rao Lower Bound) levels.
Visualization of Glutamate Decarboxylase Activity in Barley Seeds under Salinity Stress Using Mass Microscope
Authors: Ikuta Soichiro , Fukusaki Eiichiro , Shimma Shuichi
Published: December 2022

γ-Aminobutyric acid (GABA) accumulates in plants in response to environmental stresses. The activity levels of glutamate decarboxylase (GAD), an enzyme involved in GABA biosynthesis, are reported to increase during germination under salinity stress. However, it is not clear which tissues of the plant seeds are affected by GAD activity in response to salinity stress. In this study, the effects of salinity stress on the distribution of barley seeds GAD activity during germination were investigated. The mass spectrometry imaging (MSI) method was optimized, and the distribution of GAD activity in germinated seeds exposed to salinity stress at different germination stages from 12 to 48 h after imbibition was investigated. In this study, MSI was successfully applied to enzyme histochemistry to visualize the relative GAD activity in germinating barley seeds for the first time. The salinity stress increased the GAD activity, mostly due to the increase in relative GAD activity in the embryo. Higher GAD activity was detected in seeds exposed to salinity stress in the scutellum or aleurone layer, which are difficult to separate for extraction. This method can be used to clarify the role of GABA shunts, including GAD enzyme responses, in barley seeds under stress.
Impact of Plant-Beneficial Bacterial Inocula on the Resident Bacteriome: Current Knowledge and Future Perspectives
Authors: Vuolo Francesco , Novello Giorgia , Bona Elisa , Gorrasi Susanna , Gamalero Elisa
Published: December 2022

The inoculation of plant growth-promoting bacteria (PGPB) as biofertilizers is one of the most efficient and sustainable strategies of rhizosphere manipulation leading to increased plant biomass and yield and improved plant health, as well as the ameliorated nutritional value of fruits and edible seeds. During the last decades, exciting, but heterogeneous, results have been obtained growing PGPB inoculated plants under controlled, stressful, and open field conditions. On the other hand, the possible impact of the PGPB deliberate release on the resident microbiota has been less explored and the little available information is contradictory. This review aims at filling this gap: after a brief description of the main mechanisms used by PGPB, we focus our attention on the process of PGPB selection and formulation and we provide some information on the EU regulation for microbial inocula. Then, the concept of PGPB inocula as a tool for rhizosphere engineering is introduced and the possible impact of bacterial inoculant on native bacterial communities is discussed, focusing on those bacterial species that are included in the EU regulation and on other promising bacterial species that are not yet included in the EU regulation.
Transmission of Escherichia coli Causing Pyometra between Two Female Dogs
Authors: Xavier Rafael Gariglio Clark , Santana Clarissa Helena , da Silva Paloma Helena Sanches , Aburjaile Flávia Figueira , Pereira Felipe Luiz , Figueiredo Henrique César Pereira , Freitas Patrícia Maria Coletto , Santos Renato Lima , Silva Rodrigo Otávio Silveira
Published: December 2022

Despite its clinical relevance, the pathogenesis of canine pyometra remains poorly understood. To date, it is recognized as a non-transmissible infectious disease. In this study, the simultaneous occurrence of pyometra and Escherichia coli in two cohabitant female dogs underwent in-depth investigation due to the hypothesis of transmission between these animals. Two 5-year-old Chow Chow dogs (namely, dogs 23 and 24—D23 and D24) were referred to a veterinary hospital with suspected pyometra. Both animals showed prostration, anorexia, and purulent vulvar discharge over a 1-week period. After ovariohysterectomy, uterine tissue, uterine contents, and rectal swabs were collected for histopathological and microbiological analysis. Uterine histology demonstrated purulent material and multifocal necrosis with endometrial ulceration, and a morphological diagnosis of pyometra was confirmed. Furthermore, E. coli from the same phylogroup (B2) and positive for the same virulence factors with the same antimicrobial susceptibility profile was isolated from the uterine contents of both dogs and the rectum of D23. Conversely, the E. coli strains recovered from D24 differed in phylogroup (one isolate), virulence factors (all three isolates), and antimicrobial susceptibility (all three isolates). Enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) suggested that all isolates from the uterine content of both dogs and the rectal swab of D23 were 100% the same, but different from all isolates in the rectal swab of D24. One isolate from the uterine content of each animal as well as rectal swabs were subjected to whole-genome sequencing (WGS). Both whole-genome multilocus sequence typing(wgMLST) and single-nucleotide polymorphism (SNP) analysis supported the hypothesis that the isolates from the uterine content of both animals and the rectal swab of D23 were clonal. Taken together, these clinical features, pathology, microbiology, and molecular findings suggest, to the best of our knowledge, the first transmission of E. coli associated with pyometra between two animals. These results could impact the management of sites where several females cohabit in the same local area such as kennels.
Nodulation and Growth Promotion of Chickpea by Mesorhizobium Isolates from Diverse Sources
Authors: Wanjofu Edwin I. , Venter Stephanus N. , Beukes Chrizelle W. , Steenkamp Emma T. , Gwata Eastonce T. , Muema Esther K.
Published: December 2022

The cultivation of chickpea (Cicer arietinum L.) in South Africa is dependent on the application of suitable Mesorhizobium inoculants. Therefore, we evaluated the symbiotic effectiveness of several Mesorhizobium strains with different chickpea genotypes under controlled conditions. The tested parameters included shoot dry weight (SDW), nodule fresh weight (NFW), plant height, relative symbiotic effectiveness (RSE) on the plant as well as indole acetic acid (IAA) production and phosphate solubilization on the rhizobia. Twenty-one Mesorhizobium strains and six desi chickpea genotypes were laid out in a completely randomized design (CRD) with three replicates in a glasshouse pot experiment. The factors, chickpea genotype and Mesorhizobium strain, had significant effects on the measured parameters (p < 0.001) but lacked significant interactions based on the analysis of variance (ANOVA). The light variety desi genotype outperformed the other chickpea genotypes on all tested parameters. In general, inoculation with strains LMG15046, CC1192, XAP4, XAP10, and LMG14989 performed best for all the tested parameters. All the strains were able to produce IAA and solubilize phosphate except the South African field isolates, which could not solubilize phosphate. Taken together, inoculation with compatible Mesorhizobium promoted chickpea growth. This is the first study to report on chickpea-compatible Mesorhizobium strains isolated from uninoculated South African soils with no history of chickpea production; although, their plant growth promotion ability was poorer compared to some of the globally sourced strains. Since this study was conducted under controlled conditions, we recommend field studies to assess the performance of the five highlighted strains under environmental conditions in South Africa.
A Review for In Vitro and In Vivo Detection and Imaging of Gaseous Signal Molecule Carbon Monoxide by Fluorescent Probes
Authors: Xie Can , Luo Kun , Tan Libin , Yang Qiaomei , Zhao Xiongjie , Zhou Liyi
Published: December 2022

Carbon monoxide (CO) is a vital endogenous gaseous transmitter molecule involved in the regulation of various physiological and pathological processes in living biosystems. In order to investigate the biological function of CO, many technologies have been developed to monitor the level of endogenous CO in biosystems. Among them, the fluorescence detection technology based on the fluorescent probe has the advantages of high sensitivity, excellent selectivity, simple operation, especially non-invasive damage to biological samples, and the possibility of real-time in situ detection, etc., which is considered to be one of the most effective and applicable detection techniques. Therefore, in the last few years, a lot of work has been carried out on the design, synthesis and in vivo fluorescence imaging studies of CO fluorescent probes. Furthermore, using fluorescent probes to detect the changes in CO concentrations in living cells and tissues as well as in organisms has been one of the hot research topics in recent years. However, it is still a challenge to rationally design CO fluorescent probe with excellent optical performance, structural stability, low background interference, good biocompatibility, and excellent water solubility. Therefore, this review focuses on the research progress of CO fluorescent probes in the detection mechanism and biological applications in recent years. However, this popular and leading topic has rarely been summarized comprehensively to date. Thus, the research progress of CO fluorescent probes in recent years is reviewed in terms of their design concept, detection mechanism, and their biological applications. In addition, the relationship between the structure and performance of the probes was also discussed. More significantly, we hope that more excellent optical properties fluorescent probes for gaseous transmitter molecule CO detection and imaging will overcome the current problems of high biotoxicity and limited water solubility in future.
Antibacterial and Physical Properties of PVM/MA Copolymer- Incorporated Polymethyl Methacrylate as a Novel Antimicrobial Acrylic Resin Material
Authors: Lai Christopher , Nguyen Ashten , Ye Lynna , Hao Jessica , Koo Hyun , Mante Francis , Ozer Fusun
Published: December 2022

Polymethyl methacrylate (PMMA), an acrylic resin used in orthodontic appliances and removable dentures for its biocompatibility and esthetics, may harbor bacteria on its surface. The present study investigated a new PMMA formula with Gantrez: an antibacterial copolymer of methyl vinyl ether and maleic acid (PVM/MA). Samples were tested for mechanical properties (surface hardness, flexural strength, water sorption, and water solubility) and effects against Streptococcus mutans. Six groups (0%-control, 5%, 10%, 15%, 20%, and 25% Gantrez) of n = 12 were fabricated for physical property tests and analyzed with one-way ANOVA and Prism 6. From these results, three groups (0%, 5%, and 10% Gantrez) were selected for antibacterial tests, and data were analyzed with one-way ANOVA and Tukey’s multiple comparison test. Adding 5% and 10% Gantrez into PMMA significantly decreased S. mutans adhesion. There was no significant difference between the control vs. 5%, 10%, 15%, and 20% Gantrez (p > 0.05) for surface hardness, the control vs. 5% Gantrez (p > 0.05) for flexural strength, and the control vs. 5 and 10% Gantrez for water sorption and water solubility. Overall, incorporating 5% Gantrez into PMMA may be a promising solution to reduce bacterial adhesion without changing the acrylic resin’s physical properties.
PINK1/Parkin-Mediated Mitophagy Partially Protects against Inorganic Arsenic-Induced Hepatic Macrophage Polarization in Acute Arsenic-Exposed Mice
Authors: Qu Gaoyang , Liu Zi , Zhang Jiaxin , Guo Yaning , Li Hui , Qu Ruijie , Su Wei , Zhang Huan , Zhang Lin , Xu Hong , Shen Fuhai , Jiang Shoufang , Liu Heliang , Li Jinlong
Published: December 2022

Inorganic arsenic is a well-known environmental toxicant and carcinogen, and there is overwhelming evidence for an association between this metalloid poisoning and hepatic diseases. However, the biological mechanism involved is not well characterized. In the present study, we probed how inorganic arsenic modulates the hepatic polarization of macrophages, as well as roles of PTEN-induced kinase 1 (PINK1)/Parkin-mediated mitophagy participates in regulating the metalloid-mediated macrophage polarization. Our results indicate that acute arsenic exposure induced macrophage polarization with up-regulated gene expression of inducible nitric oxide synthase (Inos) and arginase-1 (Arg1), monocyte chemotactic protein-1 (Mcp-1) and macrophage inflammatory protein-2 (Mip-2), tumor necrosis factor (Tnf)-α, interleukin (Il)-1β and Il-6, as well as anti-inflammatory factors Il-4 and Il-10. In parallel, we demonstrated the disrupted hepatic redox balance typically characterized by the up-regulation of hydrogen peroxide (H2O2) and glutathione (GSH), and activation of PINK1/Parkin-mediated mitophagy in the livers of acute arsenic-exposed mice. In addition, our results demonstrate that it might be the PINK1/Parkin-mediated mitophagy that renders hepatic macrophage refractory to arsenic-induced up-regulation of the genes Inos, Mcp-1, Mip-2, Tnf-α, Il-1β, Il-6 and Il-4. In this regard, this is the first time the protective effects of PINK1/Parkin-mediated mitophagy in inorganic arsenic-induced hepatic macrophage polarization in vivo have been reported. These findings add novel insights into the arsenical immunotoxicity and provide a basis for the preve.ntive and therapeutic potential of PINK1/Parkin-mediated mitophagy in arsenic poisoning.
Phenotypic and Target-Directed Screening Yields New Acaricidal Alternatives for the Control of Ticks
Authors: Saporiti Tatiana , Cabrera Mauricio , Bentancur Josefina , Ferrari María Elisa , Cabrera Nallely , Pérez-Montfort Ruy , Aguirre-Crespo Francisco J. , Gil Jorge , Cuore Ulises , Matiadis Dimitris , Sagnou Marina , Alvarez Guzmán
Published: December 2022

Rhipicephalus microplus, the “common cattle tick”, is the most important ectoparasite in livestock worldwide due to the economic and health losses it produces. This tick is a vector for pathogens of several tick-borne diseases. In Latin American countries, damages reach approximately USD 500 million annually due to tick infections, as well as tick-borne diseases. Currently, resistant populations for every chemical group of acaricides have been reported, posing a serious problem for tick control. This study aims to find new alternatives for controlling resistant ticks with compounds derived from small synthetic organic molecules and natural origins. Using BME26 embryonic cells, we performed phenotypic screening of 44 natural extracts from 10 Mexican plants used in traditional medicine, and 33 compounds selected from our chemical collection. We found 10 extracts and 13 compounds that inhibited cell growth by 50% at 50 µg/mL and 100 µM, respectively; the dose-response profile of two of them was characterized, and these compounds were assayed in vitro against different life stages of Rhipicephalus microplus. We also performed a target-directed screening of the activity of triosephosphate isomerase, using 86 compounds selected from our chemical collection. In this collection, we found the most potent and selective inhibitor of tick triosephosphate isomerase reported until now. Two other compounds had a potent acaricidal effect in vitro using adults and larvae when compared with other acaricides such as ivermectin and Amitraz. Those compounds were also selective to the ticks compared with the cytotoxicity in mammalian cells like macrophages or bovine spermatozoids. They also had a good toxicological profile, resulting in promising acaricidal compounds for tick control in cattle raising.
Thermoresponsive Ionic Liquid with Different Cation–Anion Pairs as Draw Solutes in Forward Osmosis
Authors: Yang DaEun , Kang Hyo
Published: December 2022

We synthesized various phosphonium- and ammonium-based ionic liquids (ILs), using benzenesulfonate (BS) and 4-methylbenzenesulfonate (MBS) to establish the criteria for designing an ideal draw solute in a forward osmosis (FO) system. Additionally, the effects of monocationic, dicationic, and anionic species on FO performance were studied. Monocationic compounds ([P4444][BS], [P4444][MBS], [N4444][BS], and [N4444][MBS]) were obtained in one step via anion exchange. Dicationic compounds ([(P4444)2][BS], [(P4444)2][MBS], [(N4444)2][BS], and [(N4444)2][MBS]) were prepared in two steps via a Menshutkin SN2 reaction and anion exchange. We also investigated the suitability of ILs as draw solutes for FO systems. The aqueous [P4444][BS], [N4444][BS], [N4444][MBS], and [(N4444)2][BS] solutions did not exhibit thermoresponsive behavior. However, 20 wt% [P4444][MBS], [(P4444)2][BS], [(P4444)2][MBS], and [(N4444)2][MBS] had critical temperatures of approximately 43, 33, 22, and 60 °C, respectively, enabling their recovery using temperature. An increase in IL hydrophobicity and bulkiness reduces its miscibility with water, demonstrating that it can be used to tune its thermoresponsive properties. Moreover, the FO performance of 20 wt% aqueous [(P4444)2][MBS] solution was tested for water flux and found to be approximately 10.58 LMH with the active layer facing the draw solution mode and 9.40 LMH with the active layer facing the feed solution.
Hydroethanolic Extract of Urtica dioica L. (Stinging Nettle) Leaves as Disaccharidase Inhibitor and Glucose Transport in Caco-2 Hinderer
Authors: Altamimi Mohammad A. , Abu-Reidah Ibrahim M. , Altamimi Almothana , Jaradat Nidal
Published: December 2022

Herbal treatment for diabetes mellitus is widely used. The pharmacological activity is thought to be due to the phenolic compounds found in the plant leaves. The present study aims to investigate the phytochemical composition of Urtica dioica (UD) hydroethanolic extract and to screen its antidiabetic activity by disaccharidase hindering and glucose transport in Caco-2 cells. The results have shown that a total of 13 phenolic compounds in this work, viz. caffeic and coumaric acid esters (1, 2, 4–7, 10), ferulic derivative (3), and flavonoid glycosides (8, 9, 11–13), were identified using HPLC-DAD-ESI/MS2. The most abundant phenolic compounds were 8 (rutin) followed by 6 (caffeoylquinic acid III). Less predominant compounds were 4 (caffeoylquinic acid II) and 11 (kaempferol-O-rutinoside). The UD hydroethanolic extract showed 56%, 45%, and 28% (1.0 mg/mL) inhibition level for maltase, sucrase, and lactase, respectively. On the other hand, glucose transport was 1.48 times less at 1.0 mg/mL UD extract compared with the control containing no UD extract. The results confirmed that U. dioica is a potential antidiabetic herb having both anti-disaccharidase and glucose transport inhibitory properties, which explained the use of UD in traditional medicine.
Aluminosilicate-Supported Catalysts for the Synthesis of Cyclic Carbonates by Reaction of CO2 with the Corresponding Epoxides
Authors: Atzori Luciano , Comès Adrien , Fusaro Luca , Aprile Carmela , Cutrufello Maria Giorgia
Published: December 2022

Functionalized aluminosilicate materials were studied as catalysts for the conversion of different cyclic carbonates to the corresponding epoxides by the addition of CO2. Aluminum was incorporated in the mesostructured SBA-15 silica network. Thereafter, functionalization with imidazolium chloride or magnesium oxide was performed on the Al_SBA-15 supports. The isomorphic substitution of Si with Al and the resulting acidity of the supports were investigated via 27Al magic angle spinning (MAS) nuclear magnetic resonance (NMR) spectroscopy and NH3 adsorption microcalorimetry. The Al content and the amount of MgO were quantified via inductively coupled plasma optical emission spectroscopy (ICP-OES) analysis. The anchoring of the imidazolium salt was assessed by 29Si and 13C MAS NMR spectroscopy and quantified by combustion chemical analysis. Textural and structural properties of supports and catalysts were studied by N2 physisorption and X-ray diffraction (XRD). The functionalized systems were then tested as catalysts for the conversion of CO2 and epoxides to cyclic carbonates in a batch reactor at 100 or 125 °C, with an initial CO2 pressure (at room temperature) of 25 bar. Whereas the activity of the MgO/xAl_SBA-15 systems was moderate for the conversion of glycidol to the corresponding cyclic carbonate, the Al_SBA-15-supported imidazolium chloride catalysts gave excellent results over different epoxides (conversion of glycidol, epichlorohydrin, and styrene oxide up to 89%, 78%, and 18%, respectively). Reusability tests were also performed. Even when some deactivation from one run to the other was observed, a comparison with the literature showed the Al-containing imidazolium systems to be promising catalysts. The fully heterogeneous nature of the present catalysts, where the inorganic support on which the imidazolium species are immobilized also contains the Lewis acid sites, gives them a further advantage with respect to most of the catalytic systems reported in the literature so far.
Non-Additive Optical Response in Transition Metal Dichalcogenides Heterostructures
Authors: El-Sayed Marwa A. , Tselin Andrey P. , Ermolaev Georgy A. , Tatmyshevskiy Mikhail K. , Slavich Aleksandr S. , Yakubovsky Dmitry I. , Novikov Sergey M. , Vyshnevyy Andrey A. , Arsenin Aleksey V. , Volkov Valentyn S.
Published: December 2022

Van der Waals (vdW) heterostructures pave the way to achieve the desired material properties for a variety of applications. In this way, new scientific and industrial challenges and fundamental questions arise. One of them is whether vdW materials preserve their original optical response when assembled in a heterostructure. Here, we resolve this issue for four exemplary monolayer heterostructures: MoS2/Gr, MoS2/hBN, WS2/Gr, and WS2/hBN. Through joint Raman, ellipsometry, and reflectance spectroscopies, we discovered that heterostructures alter MoS2 and WS2 optical constants. Furthermore, despite the similarity of MoS2 and WS2 monolayers, their behavior in heterostructures is markedly different. While MoS2 has large changes, particularly above 3 eV, WS2 experiences modest changes in optical constants. We also detected a transformation from dark into bright exciton for MoS2/Gr heterostructure. In summary, our findings provide clear evidence that the optical response of heterostructures is not the sum of optical properties of its constituents.
Rational-Based Discovery of Novel β-Carboline Derivatives as Potential Antimalarials: From In Silico Identification of Novel Targets to Inhibition of Experimental Cerebral Malaria
Authors: Alves Fernanda de Moura , Bellei Jessica Correa Bezerra , Barbosa Camila de Souza , Duarte Caíque Lopes , Fonseca Amanda Luisa da , Pinto Ana Claudia de Souza , Raimundo Felipe Oliveira , Carpinter Bárbara Albuquerque , Lemos Ari Sérgio de Oliveira , Coimbra Elaine Soares , Taranto Alex Gutterres , Rocha Vinícius Novaes , de Pilla Varotti Fernando , Ribeiro Viana Gustavo Henrique , Scopel Kézia K. G.
Published: December 2022

Malaria is an infectious disease widespread in underdeveloped tropical regions. The most severe form of infection is caused by Plasmodium falciparum, which can lead to development of cerebral malaria (CM) and is responsible for deaths and significant neurocognitive sequelae throughout life. In this context and considering the emergence and spread of drug-resistant P. falciparum isolates, the search for new antimalarial candidates becomes urgent. β-carbolines alkaloids are good candidates since a wide range of biological activity for these compounds has been reported. Herein, we designed 20 chemical entities and performed an in silico virtual screening against a pool of P. falciparum molecular targets, the Brazilian Malaria Molecular Targets (BRAMMT). Seven structures showed potential to interact with PfFNR, PfPK7, PfGrx1, and PfATP6, being synthesized and evaluated for in vitro antiplasmodial activity. Among them, compounds 3–6 and 10 inhibited the growth of the W2 strain at µM concentrations, with low cytotoxicity against the human cell line. In silico physicochemical and pharmacokinetic properties were found to be favorable for oral administration. The compound 10 provided the best results against CM, with important values of parasite growth inhibition on the 5th day post-infection for both curative (67.9%) and suppressive (82%) assays. Furthermore, this compound was able to elongate mice survival and protect them against the development of the experimental model of CM (>65%). Compound 10 also induced reduction of the NO level, possibly by interaction with iNOS. Therefore, this alkaloid showed promising activity for the treatment of malaria and was able to prevent the development of experimental cerebral malaria (ECM), probably by reducing NO synthesis.
Surveillance, Diversity and Vegetative Compatibility Groups of Fusarium oxysporum f. sp. vasinfectum Collected in Cotton Fields in Australia (2017 to 2022)
Authors: Le Duy P. , Nguyen Chi P. T. , Kafle Dinesh , Scheikowski Linda , Montgomery Janelle , Lambeth Emma , Thomas Amanda , O’Keeffe Kieran , Shakeshaft Beth , Young Alison , Mckay Andrew , Twine Annabel , Hudson Elsie , Jackson Rodney , Smith Linda J.
Published: December 2022

Cotton (Gossypium hirsutum) is a billion-dollar crop in regional New South Wales (NSW) and Queensland, Australia. Fusarium wilt (FW) caused by Fusarium oxysporum f. sp. vasinfectum (Fov) is an economically important disease. Initial disease losses of up to 90% when the disease was first detected resulted in fields being taken out of cotton production. The disease is now well-managed due to the adoption of highly resistant varieties. However, annual disease surveys recently revealed that the disease dynamic has changed in the past few seasons. With relatively mild and wet weather conditions during the 2021/22 growing season, FW was detected in eight surveyed valleys in NSW and Queensland, with the disease incidence as high as 44.5% and 98.5% in individual fields in early and late seasons, respectively. Fov is genetically distinct and evolved from local Fusarium oxysporum strains. Additionally, the pathogen was reported to evolve rapidly under continuous cotton cropping pressure. However, our knowledge of the genetic composition of the prevailing population is limited. Sequences of the translation elongation factor alpha 1 (TEF1) revealed that 94% of Fusarium isolates recovered from FW-infected cotton were clustered together with known Australian Fov and relatively distant related to overseas Fov races. All these isolates, except for nine, were further confirmed positive with a specific marker based on the Secreted in Xylem 6 (SIX6) effector gene. Vegetative compatibility group (VCG) analyses of 166 arbitrarily selected isolates revealed a predominance of VCG01111. There was only one detection of VCG01112 in the Border Rivers valley where it was first described. In this study, the exotic Californian Fov race 4 strain was not detected using a specific marker based on the unique Tfo1 insertion in the phosphate (PHO) gene. This study indicated that the prevalence and abundance of Fov across NSW and Queensland in the past five seasons was probably independent of its genetic diversity.
The Science of Emotion: Mind, Body, and Culture
Authors: Mun Cecilea
Published: December 2022

In this paper, I give readers an idea of what some scholars are interested in, what I found interesting, and what may be of future interest in the philosophy of emotion. I begin with a brief overview of the general topics of interests in the philosophy of emotion. I then discuss what I believe to be some of the most interesting topics in the contemporary discourse, including questions about how philosophy can inform the science of emotion, responses to aspects of the mind–body problem, and concerns about perception, cognition, and emotion, along with questions about the place of 4E approaches and meta-semantic pluraliste approaches in the embodied cognitive tradition. I also discuss the natural kind–social construction debate in the philosophy of emotion, the emerging field of cultural evolution, the import of a dual-inheritance theory in this emerging field, and I propose a possible way to integrate the frameworks of dual-inheritance theory and meta-semantic pluralisme to demonstrate at least one way in which the philosophy of emotion can contribute to the emerging field of cultural evolution. I conclude with a brief summary of this paper and note at least one significant implication of my proposal for the natural kind–social construction debate in the philosophy of emotion.
Searching for an Enhanced Signal of the Onset of Color Transparency in Baryons with D(e,e′p)n Scattering
Authors: Li Shujie , Yero Carlos , West Jennifer Rittenhouse , Bennett Clare , Cosyn Wim , Higinbotham Douglas , Sargsian Misak , Szumila-Vance Holly
Published: December 2022

Observation of the onset of color transparency in baryons would provide a new means of studying the nuclear strong force and would be the first clear evidence of baryons transforming into a color-neutral point-like size in the nucleus as predicted by quantum chromodynamics. Recent C(e,e′p) results from electron-scattering did not observe the onset of color transparency (CT) in protons up to spacelike four-momentum transfers squared, Q2=14.2 GeV2. The traditional methods of searching for CT in (e,e′p) scattering use heavy targets favoring kinematics with already initially reduced final state interactions (FSIs) such that any CT effect that further reduces FSIs will be small. The reasoning behind this choice is the difficulty in accounting for all FSIs. D(e,e′p)n, on the other hand, has well-understood FSI contributions from double scattering with a known dependence on the kinematics and can show an increased sensitivity to hadrons in point-like configurations. Double scattering is the square of the re-scattering amplitude in which the knocked-out nucleon interacts with the spectator nucleon, a process that is suppressed in the presence of point-like configurations and is particularly well-studied for the deuteron. This suppression yields a quadratic sensitivity to CT effects and is strongly dependent on the choice of kinematics. Here, we describe a possible Jefferson National Accelerator Facility (JLab) electron-scattering experiment that utilizes these kinematics and explores the potential signal for the onset of CT with enhanced sensitivity as compared to recent experiments.
Exogenous Application of a Plant Elicitor Induces Volatile Emission in Wheat and Enhances the Attraction of an Aphid Parasitoid Aphidius gifuensis
Authors: Xiao Dianzhao , Liu Jiahui , Liu Yulong , Wang Yiwei , Zhan Yidi , Liu Yong
Published: December 2022

It is well known that plant elicitors can induce plant defense against pests. The herbivore-induced plant volatile (HIPV) methyl salicylate (MeSA), as a signaling hormone involved in plant pathogen defense, is used to recruit natural enemies to protect wheat and other crops. However, the defense mechanism remains largely unknown. Here, the headspace volatiles of wheat plants were collected and analyzed by gas chromatography-mass spectrometry (GC−MS), gas chromatography with electroantennographic detection (GC−EAD) and principal component analysis (PCA). The results showed that exogenous application of MeSA induced qualitative and quantitative changes in the volatiles emitted from wheat plants, and these changes were mainly related to Carveol, Linalool, m-Diethyl-benzene, p-Cymene, Nonanal, D-limonene and 6-methyl-5-Hepten-2-one. Then, the electroantennogram (EAG) and Y-tube bioassay were performed to test the physiological and behavioral responses of Aphidius gifuensis Ashmesd to the active volatile compounds (p-Cymene, m-Diethyl-benzene, Carveol) that identified by using GC-EAD. The female A. gifuensis showed strong physiological responses to 1 μg/μL p-Cymene and 1 μg/μL m-Diethyl-benzene. Moreover, a mixture blend was more attractive to female A. gifuensis than a single compound. These findings suggested that MeSA could induce wheat plant indirect defense against wheat aphids through attracting parasitoid in the wheat agro-ecosystem.
Conscience, Law, and Politics
Authors: Hannon Patrick
Published: December 2022

Of the many questions on which our title invites reflection, one in particular has again achieved prominence in the United States: how is a politician who is Roman Catholic expected to vote when a measure purports to legalise what church teaching says is morally wrong? The debate has become increasingly acrimonious, to the point where senior prelates are among those who contend that President Biden and Speaker of the House Pelosi are unfit to receive Holy Communion since they subscribe to the ‘pro-choice’ position of the Democratic Party. The core question has become politicised, its public discussion poisoned by association with toxic elements of the so-called ‘culture wars’, at an impasse that is impairing effective leadership in the US Catholic Church, and an effective Catholic contribution to the common good of American society. I shall argue that this is as unnecessary as it is regrettable, and that a way through the impasse is to be found in mainline Catholic thinking about morality, law, and politics.
Birds of Prey, Birds of Wisdom: Relating to Non-Humans in Contemporary Western-Based Shamanism
Authors: Ivanescu Carolina , Groskamp Nienke
Published: December 2022

Birds of prey appear frequently in contemporary forms of shamanism. For example, Michael Harner’s Core Shamanism references the ‘power animal,’ or the authentic self, which sometimes takes the form of a strong and benevolent eagle. However, precisely how meaning and belief concerning these birds may have been lost, challenged or (re)invented remains to be explored. In this contribution, we have used the methods and vision of netnography to explore the relationships between contemporary western-based, self-defined shamans and birds of prey: real, imagined or represented.