bluuebunny/crossref_metadata_2025 huggingface dataset:
Study on Failure Performance of the Thin-Walled Steel-Reinforced Concrete Pier under Low Cyclic Loading
Authors: Chen Huihui , Xu Bing , Liu Qin , Gu Jianfeng
Published: September 2022

Recently, the light-type pier can be easily observed in urban elevated roads and bridge structures. This type of pier is broadly used in the field of structural engineering. The Thin-Walled Steel-Reinforced Concrete Pier (TSRCP), as the typical pier of the light-type piers, shows a better mechanical performance than the conventional reinforced concrete pier due to the enhancement of the H-shape steel. In terms of the TSRCP, the designed parameters, including the depth–width ratio (DWR) and axial compression ratio (ACR), have been determined in this study as the concerned variables. Then, a low cyclic loading experiment is conducted based on the three test specimens to investigate the effects of the concerned variables on the failure of the specimens. Meanwhile, some comparative studies are carried out based on the failure processes and modes, critical loading values (cracking, yielding and ultimate state), strain and ductility. The obtained experimental results demonstrate that all specimens illustrate a bending failure mode under the vertical and horizontal low cyclic loadings. Furthermore, for the concerned variables, the increasing DWR will reduce the ultimate bearing capacity of the TSRCP but enhance the plastic deformation. For the ACR, the decrease positively affects the cracking load, further improving the ultimate bearing capacity; however, the deformation capacity will be restrained. Finally, Abaqus is adopted to model the failure processes of three specimens; the comparative study has been conducted based on the simulated and experimental results. After that, the effects of the concerned variables on the failure performances are discussed. Meanwhile, the horizontal ultimate bearing capacity calculated equation for the TSRCP was deduced and validated based on the experimental and simulated data; the verification proves that the proposed equation can obtain a good result. In this study, the complex calculated process of the original equation can be simplified, which not only provides a convenient and useful way to design and manufacture this type of component but can serve as a guideline for validating the practical engineering applications.
Targeting the Metabolic Rewiring in Pancreatic Cancer and Its Tumor Microenvironment
Authors: Yamamoto Keisuke , Iwadate Dosuke , Kato Hiroyuki , Nakai Yousuke , Tateishi Keisuke , Fujishiro Mitsuhiro
Published: September 2022

Pancreatic ductal adenocarcinoma (PDAC) is an aggressive malignancy with only a few effective therapeutic options. A characteristic feature of PDAC is its unique tumor microenvironment (TME), termed desmoplasia, which shows extensive fibrosis and extracellular matrix deposition, generating highly hypoxic and nutrient-deprived conditions within the tumor. To thrive in this harsh TME, PDAC undergoes extensive metabolic rewiring that includes the altered use of glucose and glutamine, constitutive activation of autophagy-lysosomal pathways, and nutrient acquisition from host cells in the TME. Notably, these properties support PDAC metabolism and mediate therapeutic resistance, including immune suppression. A deeper understanding of the unique metabolic properties of PDAC and its TME may aid in the development of novel therapeutic strategies against this deadly disease.
Early Dynamics of Quantitative SEPT9 and SHOX2 Methylation in Circulating Cell-Free Plasma DNA during Prostate Biopsy for Prostate Cancer Diagnosis
Authors: Krausewitz Philipp , Kluemper Niklas , Richter Ayk-Peter , Büttner Thomas , Kristiansen Glen , Ritter Manuel , Ellinger Jörg
Published: September 2022

Background: The methylation status of Septin 9 (SEPT9) and short stature homeobox 2 (SHOX2) in circulating cell-free DNA (ccfDNA) are validated pan-cancer biomarkers. The present proof-of-concept study aimed to investigate the potential and dynamics of quantitative SEPT9 and SHOX2 methylation in prostate cancer (PCa) patient tissue and ccfDNA during prostate biopsy as a diagnostic tool. Methods: The methylation patterns of SEPT9 and SHOX2 in prostate tissue were analyzed using The Cancer Genome Atlas data set (n = 498 PCa and n = 50 normal adjacent prostate tissue (NAT)). Next, dynamic changes of ccfDNA methylation were quantified in prospectively enrolled patients undergoing prostate biopsy (n = 72), local treatment for PCa (n = 7; radical prostatectomy and radiotherapy) as well as systemic treatment for PCa (n = 6; chemotherapy and 177-Lu-PSMA-therapy). Biomarker levels were correlated with clinicopathological parameters. Results: SEPT9 and SHOX2 were hypermethylated in PCa tissue (p < 0.001) and allowed discrimination of PCa and non-tumor prostate tissue (mSEPT9: AUC 0.87, 95%CI [0.82–0.92]; mSHOX2: AUC 0.89, 95%CI 0.84–0.94). SHOX2 methylation and mRNA levels were significantly higher in PCa tissue and increased with tumor stage and grade, as well as in patients suffering from biochemical recurrence following radical prostatectomy. SEPT9 and SHOX2 ccfDNA methylation allowed distinguishing patients with localized and metastatic disease (p < 0.001 for both). In addition, methylation levels increased shortly after prostate biopsy only in patients with PCa (ΔmSEPT9: p < 0.001 and ΔmSHOX2: p = 0.001). Conclusions: The early dynamics of methylated SEPT9 and SHOX2 in ccfDNA allow differentiation between PCa patients and patients without PCa and is a promising marker for tumor monitoring in the metastatic stage to determine tumor burden under systemic therapy.
No Impact of PolySia-NCAM Expression on Treatment Response in Neuroendocrine Neoplasms of the Lung
Authors: Gagiannis Daniel , Scheil Anna , Gagiannis Sarah , Hackenbroch Carsten , Horstkorte Ruediger , Steinestel Konrad
Published: September 2022

Background: Polysialic acids (abbr. polySia) are found on numerous tumors, including neuroendocrine lung tumors. They have previously been shown to impact metastatic potential, as they can influence the signaling and adhesion properties of neuronal cell adhesion molecules (abbr. NCAM) and other cell adhesion molecules. Therefore, the aim of this small pilot study was to analyze whether there was a correlation between polySia-NCAM expression and specific clinical or histopathologic characteristics, and if polySia-NCAM expression had an impact on treatment response, disease progression and prognosis of lung neuroendocrine neoplasms. Methods: This work was based on an analysis of 28 digitized patient records and corresponding patient samples. The response to therapy was radiologically determined at the time of diagnosis and at certain intervals during therapy following the current RECIST1.1 and volumetric sphere calculation. To analyze whether polySia-NCAM expression had prognostic relevance, polySia-NCAM-positive and -negative cases were compared in a Kaplan-Meier survival analysis. Findings: A majority of 78.6% lung neuroendocrine neoplasms showed a strong staining signal for polySia-NCAM. There was a significant correlation between expression and histopathological grade (p = 0.0140), since carcinoids were less likely polySia-NCAM-positive compared to small cell lung carcinoma (abbr. SCLC) and large cell neuroendocrine carcinomas of the lung (abbr. LCNEC). There was no significant association between polySia-NCAM expression and clinical characteristics (age: p = 0.3405; gender: p = 0.6730; smoking history: p = 0.1145; ECOG: p = 0.1756, UICC8 stage: p = 0.1182) or radiologically determined disease progression, regardless of the criteria used to categorize response (RECIST 1.1: p = 0.0759; sphere: p = 0.0580). Furthermore, polySia-NCAM expression did not affect progression-free survival (p = 0.4198) or overall survival (p = 0.6918). Interpretation: PolySia-NCAM expression was more common in high-grade compared to low-grade neuroendocrine neoplasms of the lung; however, this small pilot study failed to show an association between polySia-NCAM expression and response to therapy.
[F18]FDG PET/CT-Derived Metabolic and Volumetric Biomarkers Can Predict Response to Treatment in Locally Advanced Cervical Cancer Patients
Authors: Gill Ronit , Abdah-Bortnyak Roxolyana , Amit Amnon , Bar-Peled Uval , Keidar Zohar
Published: September 2022

(1) Purpose: Current study aimed at evaluating the relationship between quantitative metabolic and volumetric FDG PET/CT parameters and the response to definitive chemoradiation therapy in locally advanced cervical cancer patients; (2) Methods: Ninety newly diagnosed locally advanced cervical cancer patients (FIGO IB2-IVA) were investigated. All patients underwent PET/CT at staging and after treatment. Metabolic and volumetric parameters, including SUVmax, SUVmean, Total Lesion Glycolysis (TLG), and Metabolic Tumor Volume (MTV) of the primary tumor and metastatic lymph nodes were measured and compared between patients with and without complete metabolic response (CMR). A similar analysis was performed in a subgroup of FIGO IB2-IIB patients; (3) Results: SUVmax and SUVmean of the primary tumor as well as those of metastatic lymph nodes, MTV, and TLG were found to be significantly different between CMR and non-CMR patients. In a subgroup of patients with FIGO IB2-IIB disease, MTV and TLG identified women who will achieve CMR with a threshold of 31.1 cm3 for MTV and 217.8 for TLG; (4) Conclusions: PET/CT-derived quantitative metabolic and volumetric parameters are higher in locally advanced cervical cancer patients who will not respond to definitive chemoradiation therapy. Specifically, in patients who are not metastatic at staging, MTV and TLG values can serve as a predictor for treatment response and thus may alter treatment strategy.
Interfacial Electron Redistribution of FeCo2S4/N-S-rGO Boosting Bifunctional Oxygen Electrocatalysis Performance
Authors: Zhang Wen-Lin , Liu Shi-Meng , Zhang Lu-Hua , He Ting-Ting , Yu Feng-Shou
Published: September 2022

Developing bifunctional catalysts for oxygen reduction reaction (ORR) and oxygen evolution reaction (OER) is essential for the development of zinc–air batteries (ZABs), but several challenges remain in terms of bifunctional activity. FeCo2S4/N-S-rGO was prepared by in situ homogeneous growth of bimetallic sulfide FeCo2S4 on N, S-doped reduced graphene oxide. FeCo2S4/N-S-rGO exhibits a half-wave potential of 0.89 V for ORR and an overpotential of 0.26 V at 10 mA cm−2 for OER, showing significantly bifunctional activity superior to Pt/C (0.85 V) and RuO2 (0.41 V). Moreover, the FeCo2S4/N-S-rGO assembled ZAB shows a superior specific capacity and a power density of 259.13 mW cm−2. It is demonstrated that the interfacial electron redistribution between FeCo2S4 nanoparticles and heteroatom-doped rGO matrix can efficiently improve the electrochemical performance of the catalyst. The results provide new insights into the preparation of high-capability composite catalysts combining transition metal sulfides with carbon materials for applications in ZABs.
Efficient Degradation of Printing and Dyeing Wastewater by Lotus Leaf-Based Nitrogen Self-Doped Mesoporous Biochar Activated Persulfate: Synergistic Mechanism of Adsorption and Catalysis
Authors: Huo Jiaxu , Pang Xiaohui , Wei Xueyu , Sun Xiang , Liu Hongwei , Sheng Peifa , Zhu Meiqing , Yang Xiaofan
Published: September 2022

The discharge of printing and dyeing wastewater has been increasing, causing serious environmental pollution with the rapid development of the industry. Based on this, an N self-doped mesoporous lotus leaf biochar (LLC800) was prepared from lotus leaves as raw material for the activation of Persulfate (PS) to degrade wastewater from printing and dyeing. The removal rate of AO7 by PS, LLC800 and LLC800/PS systems were 0.84%, 31.11% and 99.46%, respectively. Electron paramagnetic resonance spectroscopy (EPR) and quench tests showed the presence of free radicals (•OH, SO4●− and O2●−) and nonradical (1O2) in the LLC800/PS system, where nonradicals (1O2) play an important role in the degradation of AO7. The “N self-doped” effect formed by the high N content of lotus leaves is the main factor leading to the high adsorption and catalytic performance of lotus leaf biochar. The effect of pyrolysis temperature on the performance of biochar can be attributed to the change of N content and conformation and specific surface area in biochar. Moreover, the LLC800/PS system has a strong resistance to interference. This work can provide technical support for the preparation of high-performance adsorption-catalytic biochar and the development of high-performance activation materials for persulfate.
Silver(I)-Catalyzed C4-H Amination of 1-Naphthylamine Derivatives with Azodicarboxylates at Room Temperature
Authors: Zhang Yuxue , Pei Mengxue , Yang Fan , Wu Yangjie
Published: September 2022

A highly facile and efficient protocol for silver(I)-catalyzed C4–H amination of 1-naphthylamine derivatives with readily available azodicarboxylates utilizing picolinamide as a bidentate directing group is reported, providing an alternative strategy for the synthesis of 4-aminated 1-naphthylamine derivatives. The reaction proceeded smoothly in acetone at room temperature undergoing a self-redox process under the base and external oxidant-free conditions, affording the desired products with good to excellent yields.
In-Depth Characterization of Debranching Type I Pullulanase from Priestia koreensis HL12 as Potential Biocatalyst for Starch Saccharification and Modification
Authors: Prongjit Daran , Lekakarn Hataikarn , Bunterngsook Benjarat , Aiewviriyasakul Katesuda , Sritusnee Wipawee , Arunrattanamook Nattapol , Champreda Verawat
Published: September 2022

Pullulanase is an effective starch debranching enzyme widely used in starch saccharification and modification. In this work, the biochemical characteristics and potential application of a new type I pullulanase from Priestia koreensis HL12 (HL12Pul) were evaluated and reported for the first time. Through in-depth evolutionary analysis, HL12Pul was classified as type I pullulanase belonging to glycoside hydrolase family 13, subfamily 14 (GH13_14). HL12Pul comprises multi-domains architecture, including two carbohydrate-binding domains, CBM68 and CBM48, at the N-terminus, the TIM barrel structure of glycoside hydrolase family 13 (GH13) and C-domain. Based on sequence analysis and experimental cleavage profile, HL12Pul specifically hydrolyzes only α-1,6 glycosidic linkage-rich substrates. The enzyme optimally works at 40 °C, pH 6.0, with the maximum specific activity of 181.14 ± 3.55 U/mg protein and catalytic efficiency (kcat/Km) of 49.39 mL/mg·s toward pullulan. In addition, HL12Pul worked in synergy with raw starch-degrading α-amylase, promoting raw cassava starch hydrolysis and increasing the sugar yield by 2.9-fold in comparison to the α-amylase alone in a short reaction time. Furthermore, HL12Pul effectively produces type III-resistant starch (RSIII) from cassava starch with a production yield of 70%. These indicate that HL12Pul has the potential as a biocatalyst for starch saccharification and modification.
Human Adipose Mesenchymal Stromal/Stem Cells Improve Fat Transplantation Performance
Authors: Piccinno Maria Serena , Petrachi Tiziana , Pignatti Marco , Murgia Alba , Grisendi Giulia , Candini Olivia , Resca Elisa , Bergamini Valentina , Ganzerli Francesco , Portone Alberto , Mastrolia Ilenia , Chiavelli Chiara , Castelli Ilaria , Bernabei Daniela , Tagliazucchi Mara , Bonetti Elisa , Lolli Francesca , De Santis Giorgio , Dominici Massimo , Veronesi Elena
Published: September 2022

The resorption rate of autologous fat transfer (AFT) is 40–60% of the implanted tissue, requiring new surgical strategies for tissue reconstruction. We previously demonstrated in a rabbit model that AFT may be empowered by adipose-derived mesenchymal stromal/stem cells (AD-MSCs), which improve graft persistence by exerting proangiogenic/anti-inflammatory effects. However, their fate after implantation requires more investigation. We report a xenograft model of adipose tissue engineering in which NOD/SCID mice underwent AFT with/without human autologous AD-MSCs and were monitored for 180 days (d). The effect of AD-MSCs on AFT grafting was also monitored by evaluating the expression of CD31 and F4/80 markers. Green fluorescent protein-positive AD-MSCs (AD-MSC-GFP) were detected in fibroblastoid cells 7 days after transplantation and in mature adipocytes at 60 days, indicating both persistence and differentiation of the implanted cells. This evidence also correlated with the persistence of a higher graft weight in AFT-AD-MSC compared to AFT alone treated mice. An observation up to 180 d revealed a lower resorption rate and reduced lipidic cyst formation in the AFT-AD-MSC group, suggesting a long-term action of AD-MSCs in support of AFT performance and an anti-inflammatory/proangiogenic activity. Together, these data indicate the protective role of adipose progenitors in autologous AFT tissue resorption.
Reprogramming—Evolving Path to Functional Surrogate β-Cells
Authors: Kalo Eric , Read Scott , Ahlenstiel Golo
Published: September 2022

Numerous cell sources are being explored to replenish functional β-cell mass since the proof-of -concept for cell therapy of diabetes was laid down by transplantation of islets. Many of these cell sources have been shown to possess a degree of plasticity permitting differentiation along new lineages into insulin-secreting β-cells. In this review, we explore emerging reprograming pathways that aim to generate bone fide insulin producing cells. We focus on small molecules and key transcriptional regulators that orchestrate phenotypic conversion and maintenance of engineered cells.
A Dialogue in the Medical Perspective—Body Mass and Nutritional Status Disorders during the Development Period
Authors: Kozioł Karolina , Kazek Beata , Sikora Dorota , Brzóska Anna , Paprocka Justyna , Emich-Widera Ewa
Published: September 2022

Eating disorders among children and youth are a serious social problem. The time of development is the starting point in shaping eating patterns. Proper nutrition provides the basis for psychophysical development. A knowledgeable pediatrician can improve society’s health by engaging parents and, later, the child or youth. We offer knowledge on the nutrition basics and the commonly available tools to assess the nutritional status. We will discuss the characteristics of eating and body mass disorders in developing children. We will provide information on the warning signals of eating and body mass disorders and recommend prophylaxis. The reader will be familiarized with the motivational dialogue as an effective control tool for the discussed health issues.
Bis(1,2,3-trimethylpyridinium) Octa(μ2-bromido)Tetrabromidopentacuprate(II): Linear, Quasi-Planar Pentacopper(II) Oligomers Stacked to Form Egg-Tray Layers
Authors: Akkina Subhash , Bond Marcus R.
Published: September 2022

The crystal structure of (1,2,3-trimethylpyridnium)2Cu5Br12 provides the second reported example of a fully halogenated, linear, quasi-planar, bibridged pentacopper(II) oligomer. The oligomers are aggregated into crosshatched layers that defy traditional notions and notations for quasi-planar oligomer stacking. The regularly arranged voids in the layers are occupied by inversion-related organic cation pairs similar to eggs in an egg-tray. The cross-hatched layer structure arises from a particular stacking of mixed organic cation/pentacopper oligomer sheets. The sheets consist of oligomers placed in a herringbone arrangement separated by zipper-like ribbons of organic cations in a structural motif similar to that found in other 1,2,3- or 1,2,6-trimethylpyridinium halidocuprate(II) structures. Alternative stacking of the sheets leads, on the other hand, to a conventional stacking pattern that conforms to traditional stacking descriptions. Interpretation of these structures in terms of the stacking of mixed cation/anion sheets, as is often performed for ABX3 systems, provides a complementary method for understanding these structures as well as providing a means to describe systems that are not easily described by traditional stacking notation.
Local Materials as a Means of Improving Motivation to EFL Learning in Kazakhstan Universities
Authors: Tazhitova Gulzhakhan , Kurmanayeva Dina , Kalkeeva Kamaryash , Sagimbayeva Jannat , Kassymbekova Nurilya
Published: September 2022

This study explores whether incorporating local materials into EFL instruction has a positive effect on students’ motivation to learning a foreign language. The main purpose of this article is to show that if the content of foreign language teaching is systematized and constructed on local materials, then the content of foreign language teaching will contribute to an increase in motivation to learning a foreign language. This experimental method was used in order to identify the level of development of educational motivation in the course of using local materials. The results of the experiment showed that participants in the experimental group were more successful than those in the control group at the conclusion of the study, providing convincing evidence that the integration of local materials has a significant impact on increasing the motivation of EFL students to learning a foreign language.
Predictors of the Effectiveness of Different Approaches to Pandemic Distance Learning
Authors: Kohout Jiří , Buršíková Dana , Frank Jan , Lukavský Jindřich , Masopust Pavel , Motlíková Iva , Rohlikova Lucie , Slavík Jan , Stacke Václav , Vejvodová Jana , Voltrová Michaela
Published: September 2022

Significant attention has been devoted to the forced switch to distance learning as a result of the COVID-19 pandemic. However, some aspects of this issue that are very important for practice are still understudied. The aim of this study is to describe the development of an online-available screening tool which could help the teachers to identify the students at risk of lowered effectiveness during the distance learning and also to select an appropriate teaching approach for the given class. A complex survey involving 35 teachers of Czech language, German language, Mathematics, Physics and Geography, and more than 1400 of their students from 70 classes, was carried out. In the first step, we identified which out of the more than 100 potentially relevant variables have predictive value for the effectiveness of distance learning. Subsequently, a series of multilinear regression models enabling to quantify the impact of the individual variables on effectiveness and perceived usefulness of distance learning were developed. Moderation analysis was also used to model how suitable synchronous and asynchronous activities based on active learning are for classes with different characteristics. Based on the results of the models, a simple screening tool helping teachers to tailor their approach and strategy is being developed.
Local and Network Dynamics of a Non-Integer Order Resistor–Capacitor Shunted Josephson Junction Oscillators
Authors: Kanagaraj Sathiyadevi , Durairaj Premraj , Prince A. Amalin , Rajagopal Karthikeyan
Published: September 2022

Spiral waves are an intriguing phenomenon that can be found in a variety of chemical and biological systems. We consider the fractional-order resistor–capacitor shunted Josephson junction chaotic oscillator to investigate the spiral wave pattern. For a preliminary understanding, we look at the dynamics of isolated FJJs and show that infinitely coexisting periodic and chaotic attractors depend on the fractional order. In addition, we perform bifurcation analysis to show the dynamical transition of the attractors as a function of fractional order and basin stability analysis to show the infinitely coexisting attractors. This is followed by the existence of spiral waves which is observed under various intrinsic and extrinsic system parameters. Finally, the impact of noise on SW is also analyzed by dispersing it to the entire stimulation period or defined time-period.
CORB2I-SLAM: An Adaptive Collaborative Visual-Inertial SLAM for Multiple Robots
Authors: Saha Arindam , Dhara Bibhas Chandra , Umer Saiyed , AlZubi Ahmad Ali , Alanazi Jazem Mutared , Yurii Kulakov
Published: September 2022

The generation of robust global maps of an unknown cluttered environment through a collaborative robotic framework is challenging. We present a collaborative SLAM framework, CORB2I-SLAM, in which each participating robot carries a camera (monocular/stereo/RGB-D) and an inertial sensor to run odometry. A centralized server stores all the maps and executes processor-intensive tasks, e.g., loop closing, map merging, and global optimization. The proposed framework uses well-established Visual-Inertial Odometry (VIO), and can be adapted to use Visual Odometry (VO) when the measurements from inertial sensors are noisy. The proposed system solves certain disadvantages of odometry-based systems such as erroneous pose estimation due to incorrect feature selection or losing track due to abrupt camera motion and provides a more accurate result. We perform feasibility tests on real robot autonomy and extensively validate the accuracy of CORB2I-SLAM on benchmark data sequences. We also evaluate its scalability and applicability in terms of the number of participating robots and network requirements, respectively.
Design of a Power Splitter Based on a 3D MMI Coupler at the Fibre-Tip
Authors: Ziman Martin , Feiler Martin , Mizera Tomas , Kuzma Anton , Pudis Dusan , Uherek Frantisek
Published: September 2022

Planar MMI couplers based on inorganic material platforms have played an essential role in photonic integrated circuits development. Advances in organic polymer fabrication techniques enable the design of components beyond a single plane, thus facilitating vertical integration for a wide range of components, including the MMI coupler. This paper presents the design of two 3D IP-dip polymer-based MMI power splitters operating in the near-infrared part of the spectrum at a wavelength of 1550 nm. The resulting output power ratio, modal field distributions, spectral characteristics, and the effects of input fibre misalignment are investigated using the beam propagation method. The fabrication method used to realise the designed splitters was direct laser writing. The function of the splitters was then verified by a highly resolved near-field scanning optical microscope.
Multi-Sensory Experience and Preferences for Children in an Urban Forest Park: A Case Study of Maofeng Mountain Forest Park in Guangzhou, China
Authors: Xu Jian , Chen Lingyi , Liu Tingru , Wang Tao , Li Muchun , Wu Zhicai
Published: September 2022

This study developed an analysis based on children’s multi-sensory experiences and preferences in urban forest park to make practical suggestions for the design of children’s activity areas. Taking Maofeng Mountain Forest Park in Guangzhou, Guangdong province as a case study, based on a face-to-face survey and online questionnaire survey, this study analyzed children’s multi-sensory landscape preferences in the park and explored the influence of multi-sensory experiences on children’s behavioral experience by establishing a structural equation model. The results reveal that visual, auditory, tactile and olfactory sensations were significantly correlated with children’s behavioral experiences. In terms of landscape preference, children preferred landscapes in blue-green tones, original building materials and challenging entertainment programs. Based on these analysis results, the design recommendations for children’s activity areas in urban forest parks are discussed.
Comparison of Superchilling and Supercooling on Extending the Fresh Quality of Beef Loin
Authors: Kim Honggyun , Hong Geun-Pyo
Published: September 2022

This study compared the effects of superchilling and supercooling preservations for 15 days on the freshness and quality characteristics of beef loin. Beef freshness was evaluated by total aerobic count (TAC), total volatile basic nitrogen (TVB-N), and thiobarbituric acid reactive substances (TBARS), and instrumental color, drip loss, cooking loss, and texture profile analysis (TPA) were determined as quality parameters. All assays were compared with fresh control and normal chilling conditions (4 °C). The mean preservation temperatures of superchilling and supercooling were −3.9 °C and −2.1 °C, respectively. The freshness parameters indicated that both superchilling and supercooling extended the freshness of beef loin for 15 days, while chilled beef could not maintain the standard of freshness conditions. For quality parameters, there was no difference between the control and supercooling treatments, whereas superchilling exhibited higher drip loss and toughness compared to the control (p < 0.05). Therefore, this study demonstrated that supercooling was the best preservation technique to extend the freshness and quality of beef loin, but superchilling was not suitable to guarantee the quality of beef.
Cocoa Spread with Grape Seed Oil and Encapsulated Grape Seed Extract: Impact on Physical Properties, Sensory Characteristics and Polyphenol Content
Authors: Lončarević Ivana , Petrović Jovana , Teslić Nemanja , Nikolić Ivana , Maravić Nikola , Pajin Biljana , Pavlić Branimir
Published: September 2022

The aim of this study was to utilize grape pomace, as a polyphenol-rich by-product of wine production, in the manufacture of enriched cocoa spread. The formulation of the cocoa spread has been modified by substitution of refined sunflower oil with cold-pressed grape seed oil. The spread with grape seed oil (Cg) was further enriched with grape seed extract encapsulated on maltodextrins (E), where 10% and 15% of E was added to Cg obtaining the samples Cg10 and Cg15. The results showed an increase in volume-weighted mean in spread samples, from 19.17 μm in Cg to 19.71 μm in Cg10 and 21.04 μm in Cg15. Casson yield stress and Casson viscosity significantly (p ˂ 0.05) increased from 16.41 Pa and 1.58 Pa·s in Cg to 29.45 Pa and 5.70 Pa·s in Cg15 due to the reduction of the fat-phase content in enriched spreads. The addition of E had no significant effect on the melting temperature (Tpeak) of the enriched spreads, while increasing the amount of E significantly (p ˂ 0.05) increased their hardness. The incorporation of grape seed oil in the cocoa spread formulation contributed to an increase in total polyphenols and flavonoids. Moreover, the addition of 10% and 15% of E to Cg resulted in approximately 1.5× and 2× higher content of phenolic compounds in Cg10 and Cg15 compared to control spread with sunflower oil (Cs). Flavonoids increased from 0.43 mg CE/g in Cs to 0.74 mg CE/g in Cg 10 and 1.24 mg CE/g in Cg15. Encapsulates positively affected sensory characteristics of enriched spread samples by reducing their grape seed oil aroma and sweetness.
Actinidin in Green and SunGold Kiwifruit Improves Digestion of Alternative Proteins—An In Vitro Investigation
Authors: Kaur Lovedeep , Mao Boning , Bailly Justine , Oladeji Olawunmi , Blatchford Paul , McNabb Warren C.
Published: September 2022

Both Hayward (green) and SunGold (gold) kiwifruit varieties contain a proteolytic enzyme, actinidin, that has been reported to enhance the upper tract digestion of animal proteins. Unlike the other gold varieties, which do not contain any actinidin, the SunGold variety contains significantly higher actinidin activity, but its activity is still much lower than that present in the green (Hayward) fruit. The objective of this study was to determine the effectiveness of actinidin in Hayward and SunGold kiwifruit in digesting alternative proteins, including pea protein, almonds, tofu, and quinoa. The protein sources were digested using a three-stage in vitro oral-gastro-small intestinal digestion model. The findings showed that both kiwifruit extracts enhanced the breakdown (observed through SDS-PAGE) for all the studied protein sources, particularly during gastric digestion, possibly due to higher actinidin activity at gastric pH. The increase in the rate of protein breakdown was probably due to the broader specificity of actinidin compared to pepsin. For many protein sources, most of the intact proteins disappeared within the first few minutes of gastric digestion with added kiwifruit extract. Green kiwifruit extract, due to its higher actinidin activity, had a higher effect on protein breakdown than the SunGold extract. However, for some proteins and under certain digestion conditions, SunGold extract resulted in higher protein breakdown. The latter, in the absence of any digestive enzymes, also led to some protein breakdown during the small intestinal digestion phase, which was not the case for the green kiwifruit extract. The green kiwifruit extract led to the greater breakdown of polypeptide chains of Pru-du 6, a major allergen in almonds. The results, for the first time, suggest that both Hayward and SunGold kiwifruit can lead to improved breakdown and digestion of alternative proteins when consumed as part of a meal; and therefore, have the potential to be used as a digestive aid in population groups looking to achieve faster and greater protein digestion such as athletes, elderly and people with the impaired digestive system.
Formation-Damage Mechanism and Gel-Breaker-Free Drill-In Fluid for Carbonate Reservoir
Authors: Fang Qingchao , Zhao Xin , Sun Hao , Wang Zhiwei , Qiu Zhengsong , Shan Kai , Ren Xiaoxia
Published: September 2022

Abundant oil and gas reserves have been proved in carbonates, but formation damage affects their production. In this study, the characteristics and formation-damage mechanism of the carbonate reservoir formation of the MS Oilfield in the Middle East were analyzed—utilizing X-ray diffraction, a scanning electron microscope, slice identification, and mercury intrusion—and technical measures for preventing formation damage were proposed. An ‘improved ideal filling for temporary plugging’ theory was introduced, to design the particle size distribution of acid-soluble temporary plugging agents; a water-based drill-in fluid, which did not require gel-breaker treatment, was formed, and the properties of the drill-in fluid were tested. The results showed that the overall porosity and permeability of the carbonate reservoir formation were low, and that there was a potential for water-blocking damage. There were micro-fractures with a width of 80–120 μm in the formation, which provided channels for drill-in fluid invasion. The average content of dolomite is 90.25%, and precipitation may occur under alkaline conditions. The polymeric drill-in fluid had good rheological and filtration properties, and the removal rate of the filter cake reached 78.1% in the chelating acid completion fluid without using gel breakers. In the permeability plugging test, the drill-in fluid formed a tight plugging zone on the surface of the ceramic disc with a pore size up to 120 μm, and mitigated the fluid loss. In core flow tests, the drill-in fluid also effectively plugged the formation core samples by forming a thin plugging layer, which could be removed by the chelating acid completion fluid, indicated by return permeability higher than 80%. The results indicated that the drill-in fluid could mitigate formation damage without the treatment of gel breakers, thus improving the operating efficiency and safety.
Identification and Quantitation of Novel ABI3 Isoforms Relative to Alzheimer’s Disease Genetics and Neuropathology
Authors: Turner Andrew K. , Shaw Benjamin C. , Simpson James F. , Estus Steven
Published: September 2022

Elucidating the actions of genetic polymorphisms associated with the risk of Alzheimer’s disease (AD) may provide novel insights into underlying mechanisms. Two polymorphisms have implicated ABI3 as a modulator of AD risk. Here, we sought to identify ABI3 isoforms expressed in human AD and non-AD brain, quantify the more abundant isoforms as a function of AD genetics and neuropathology, and provide an initial in vitro characterization of the proteins produced by these novel isoforms. We report that ABI3 expression is increased with AD neuropathology but not associated with AD genetics. Single-cell RNAseq of APP/PS1 mice showed that Abi3 is primarily expressed by microglia, including disease-associated microglia. In human brain, several novel ABI3 isoforms were identified, including isoforms with partial or complete loss of exon 6. Expression of these isoforms correlated tightly with total ABI3 expression but were not influenced by AD genetics. Lastly, we performed an initial characterization of these isoforms in transfected cells and found that, while full-length ABI3 was expressed in a dispersed punctate fashion within the cytosol, isoforms lacking most or all of exon six tended to form extensive protein aggregates. In summary, ABI3 expression is restricted to microglia, is increased with Alzheimer’s neuropathology, and includes several isoforms that display a variable tendency to aggregate when expressed in vitro.
Primary Bone Marrow HIV-Associated Hodgkin Lymphoma Complicated by Hemophagocytic Lymphohistiocytosis
Authors: Lin Ji , George Binsah
Published: September 2022

The presentation of human immunodeficiency virus (HIV)-associated Hodgkin lymphoma can differ from that of the general population. More specifically, primary bone marrow Hodgkin lymphoma is an uncommon presentation that is more often reported in patients with HIV. Given the many overlapping symptoms of Hodgkin lymphoma and HIV as well as HIV-associated infections, diagnosis can be difficult and delayed. We describe a case of primary bone marrow HIV-associated Hodgkin lymphoma complicated by hemophagocytic lymphohistiocytosis (HLH) where the initial work-up was inconclusive. Our case demonstrates the importance of early consideration of HLH as well as the need for an early bone marrow biopsy in a cytopenic patient with a fever of unknown origin.
The Gain and Loss of Cryptochrome/Photolyase Family Members during Evolution
Authors: Deppisch Peter , Helfrich-Förster Charlotte , Senthilan Pingkalai R.
Published: September 2022

The cryptochrome/photolyase (CRY/PL) family represents an ancient group of proteins fulfilling two fundamental functions. While photolyases repair UV-induced DNA damages, cryptochromes mainly influence the circadian clock. In this study, we took advantage of the large number of already sequenced and annotated genes available in databases and systematically searched for the protein sequences of CRY/PL family members in all taxonomic groups primarily focusing on metazoans and limiting the number of species per taxonomic order to five. Using BLASTP searches and subsequent phylogenetic tree and motif analyses, we identified five distinct photolyases (CPDI, CPDII, CPDIII, 6-4 photolyase, and the plant photolyase PPL) and six cryptochrome subfamilies (DASH-CRY, mammalian-type MCRY, Drosophila-type DCRY, cnidarian-specific ACRY, plant-specific PCRY, and the putative magnetoreceptor CRY4. Manually assigning the CRY/PL subfamilies to the species studied, we have noted that over evolutionary history, an initial increase of various CRY/PL subfamilies was followed by a decrease and specialization. Thus, in more primitive organisms (e.g., bacteria, archaea, simple eukaryotes, and in basal metazoans), we find relatively few CRY/PL members. As species become more evolved (e.g., cnidarians, mollusks, echinoderms, etc.), the CRY/PL repertoire also increases, whereas it appears to decrease again in more recent organisms (humans, fruit flies, etc.). Moreover, our study indicates that all cryptochromes, although largely active in the circadian clock, arose independently from different photolyases, explaining their different modes of action.
Chemical Composition and Cytoprotective Activities of Methanolic Extract of Asplenium adiantum-nigrum L. (Aspleniaceae)
Authors: Farràs Adrià , López Víctor , Maggi Filippo , Caprioli Giovanni , Vinardell María Pilar , Mitjans Montserrat
Published: September 2022

Ferns can be a source of polyphenolic compounds, with the fronds being the main parts described in ethnopharmacological studies. The present study screened polyphenolic phytochemicals and evaluated in vitro activities of a methanolic extract of Asplenium adiantum-nigrum L. fronds (AAM), an Aspleniaceae fern collected from the Prades mountains (Tarragona, Spain). Phytocharacterization by HPLC-MS/MS confirmed that the major flavonoids isolated in AAM are flavanols while the major phytochemicals are phenol acids, with chlorogenic acid being the most representative one. Cytotoxicity, cytoprotection, cellular repair activity, and phototoxicity were determined in vitro in the presence of 0.01, 0.1, and 1 mg/mL of the extract. No cytotoxicity was obtained in any of the cell lines tested: non-tumoral (3T3 and HaCaT) and tumoral (HeLa, HepG2, and A549) cells. Additionally, the polyphenolic extract showed greater protective effect against H2O2 in 3T3 than HaCaT cells. Despite the low total phenolic content of AAM (1405.68 mg phenolic phytochemicals/kg dry extract), the cytoprotective activity of this extract could be associated with the synergistic antioxidant action of their polyphenolic profile. In addition, the extract did not present phototoxicity against the non-cytotoxic 1.8 J/cm2 dose of UVA light in both non-tumoral cell lines.
Low Tree Vigor, Free Palmette Training Form, and High Planting Density Increase Olive and Oil Yield Efficiency in Dry, Sloping Areas of Mediterranean Regions
Authors: Massenti Roberto , Ioppolo Antonino , Veneziani Gianluca , Selvaggini Roberto , Servili Maurizio , Lo Bianco Riccardo , Caruso Tiziano
Published: September 2022

Exploiting biodiversity must be considered today an effective strategy to improve the sustainability of olive production systems. The evaluation of local cultivars, based on their vegetative and fruiting traits, along with an analysis of product quality, may contribute significantly to the development and diffusion of new olive-growing systems. The aim of this study was to evaluate growth, productivity, and olive oil quality of three Sicilian cultivars with different vigor/growth habit grown in four different combinations of training form and planting density. ‘Abunara’, ‘Calatina’, and ‘Nocellara del Belice’ olive trees were planted in four different intensive planting systems: 2 × 5 m trained to central leader (CLx2), 3 × 5 m trained to free palmette (FPx3), 4 × 5 m trained to small globe vase (GVx4), and 5 × 5 m trained to poly-conic vase (PVx5) and evaluated for seven years. Planting systems with low-density showed faster growth (trunk cross section area and canopy volume) than high-density systems. High-density systems had higher yield per hectare but lower yield per tree than low-density systems. ‘Calatina’ was the least vigorous but most productive cultivar. ‘Abunara’ and ‘Nocellara’ were relatively vigorous and suffered the tight spacings of high-density systems. Yield efficiency was generally high in ‘Calatina’, and it showed an increase with time in ‘Abunara’ and ‘Nocellara’ grown under the GVx4 and PVx5 systems. Fruit yield per hectare was highest in ‘Calatina’ grown under high-density systems. Oil yield was lower in ‘Nocellara’ than in ‘Abunara’ and ‘Calatina’. In terms of oil quality, ‘Calatina’ and ‘Abunara’ produced oils with higher oleic acid content than ‘Nocellara’. Generally, ‘Calatina’ has production characteristics of considerable interest, which, combined with low canopy volume and vigor, make it suitable for intensive pedestrian olive orchards with high levels of harvest and pruning mechanization and using different strategies and machines. Overall, for their management flexibility, these planting systems can contribute to improve sustainability of the olive industry even in sloping, dry areas of the Mediterranean.
Mosquito (Diptera: Culicidae) Diversity and Community Structure in Doi Inthanon National Park, Northern Thailand
Authors: Srisuka Wichai , Sulin Chayanit , Sommitr Wirat , Rattanarithikul Rampa , Aupalee Kittipat , Saeung Atiporn , Harbach Ralph E.
Published: September 2022

Urbanization and human activities create new suitable aquatic habitats for the immature stages of mosquitoes in many countries. This also applies to Doi Inthanon National Park in northern Thailand, which is named for the highest mountain in the country. Despite its popularity, there is no information regarding mosquito diversity and community structure in the different ecosystems of the park. Monthly collections of immature stages from various habitats were conducted from August 2004 to December 2005 using dipping and sucking methods. The specimens collected from each habitat were reared to adults and identified based on their morphology. Diversity parameters and community structure were statistically analyzed. A total of 140 species (3795 specimens) belonging to 15 genera were identified. Among these, four genera (Culex, Aedes, Anopheles, and Uranotaenia) had high species richness, each represented by 48, 27, 19, and 15 species, respectively. Aedes albopictus was the most relatively abundant species, representing 6.7% of the total number of captured specimens, followed by Tripteroides aranoides (5.6%) and Cx. mimulus (5%). Species richness in natural habitats was significantly higher than in artificial containers. Species richness and abundance were highest in the rainy season. In comparison to agricultural areas and villages, mosquito diversity was found to be higher in forest areas. Ground pools, stream pools, rock pools, bamboo stumps, bamboo internode, and rice fields were the most preferred natural habitats. The results indicate that Doi Inthanon National Park has a high mosquito diversity. Each species exhibits differences in abundance and distribution in different habitats, which is useful information for planning conservation measures and vector control in the park.
Staged Hybrid Repair of a Complex Type B Aortic Dissection
Authors: Șulea Cristina-Maria , Csobay-Novák Csaba , Oláh Zoltán , Banga Péter , Szeberin Zoltán , Soltész Ádám , Jokkel Zsófia , Benke Kálmán , Csonka Máté , Merkel Eperke Dóra , Merkely Béla , Szabolcs Zoltán , Pólos Miklós
Published: September 2022

Due to its heterogeneous clinical picture and lengthy evolution, the management of type B aortic dissection represents a clinical challenge, often calling for complex strategies combining medical, endovascular, and open surgical strategies. We present the case of a 45-year-old female who had previously suffered a complicated type B aortic dissection requiring a femoro-femoral crossover bypass and further conservative treatment. Seven years later, due to an aneurysmal development, a staged descending aortic management was strategized, beginning with the implantation of a frozen elephant trunk device due to an insufficient proximal landing zone for endovascular repair. However, the development of a distal stent graft-induced new entry complicated the dissection and led to the formation of a second false lumen, thus prompting an expedited hybrid reconstruction. We describe a hybrid repair strategy tailored to the patient’s particular aortic anatomic conformation, combining ilio-visceral debranching and thoracic endovascular aortic repair. Due to a lack of consensus on the ideal management strategy for type B aortic dissection, an individualized approach conducted by an experienced aortic team may generate the best outcome. The appropriate timing and planning of the intervention are the keys to successful results in complex type B aortic dissection cases with an elaborate anatomic conformation.