bluuebunny/crossref_metadata_2025 huggingface dataset:
Church and Bioethics in Greece
Authors: Nikolaos Metropolitan
Published: November 2011

The Bioethics Committee of the Church of Greece, headed by Metropolitan Nikolaos of Mesogaia and Lavreotiki, was founded a few years ago, bringing together clergy, medical and legal experts. In his address, Metropolitan Nikolaos explains the concerns and the conditions that led to its foundation and he outlines some of the activities and the principles of the Committee. This address, a somewhat broad-brush presentation of the state of bioethics in Greece, is offered as a proposal as to how the Church can work within a secular state, and how it can attempt to raise awareness about the spiritual aspect of bioethical concerns. Overall, the Bioethics Committee tries to promote public dialogue about sensitive bioethical issues, and to make sure that the spiritual perspective is duly informed by medical science. In addition, the Committee tries to make sure that the spiritual perspective is considered by the state in the face of existing or emerging law that touches on bioethical concerns.
The Ethics of Assisted Dying: A Case for a Recovery of Prudence Among the Virtues
Authors: Deane-Drummond Celia
Published: November 2011

The starting point for discussion in this paper is a case study, namely that of the controversy surrounding the case of withdrawing feeding from Eluana Englaro who had been in a permanent vegetative state (PVS) for seventeen years. I press the case for a recovery of classical prudence or practical wisdom, as understood by Thomas Aquinas, rather than beginning with deontological or utilitarian arguments. I suggest that prudence has relevance not just for specific issues concerned with cases involving PVS, but also addresses wider problems raised by secular end-of-life ethical discourse, with its emphasis on individual choice on the one hand and concerns about the slippery slope towards more liberal legislation on the other hand. Greater attention to the hospice movement is, I suggest, ambiguous in ethical terms. Finally, I argue that one of the strengths of a Thomistic understanding of prudence is its deep connection with the life of grace as that informed by consideration of the work of the Holy Spirit. This incorporates a theological element without falling into the trap of a deontology that can be dismissed by those outside the household of faith, while allowing a Christian witness to persist in such a contested public arena.
Medical Ethics and Medical Law: The Russian Experience
Authors: Siluyanova Irina
Published: November 2011

The correlation between medical ethics and medical law, while seemingly far removed from the context of Eastern Orthodoxy, is in fact of deep theological significance and eschatological prominence and has become increasingly a matter of concern in contemporary Russia. The following study examines different modes of this correlation and their moral implications for the wider society.
Mesh generation and adjustment methods for creating a halftone image on a deep-drawn cap
Authors: Huang Yih-Ping , Lin Jeng-Wen
Published: November 2011

It is desirable for deep-drawn bottle caps to have counterfeit-proof characteristics in addition to a delicate figurative design. This article presents mesh generation and adjustment methods to produce a delicate halftone image on a deep-drawn cap, so as to create a counterfeit-proof seal that solves the problem of the occurrence fake products in the cap industry. Because cap images are very sensitive, any defects in the image processing are easily noticeable to the naked eye. An image generation process used for drawn and redrawn two-piece food cans is introduced that creates a distorted image for printing onto a plane blank metal sheet. Along with the can formation and drawing process, the printed distorted image is deformed in order to result in the desired finished form. Using a similar but relatively difficult technique, the process for creating an image on a deep-drawn cap is discussed, whereby the mesh generation methods and the associated adjustment techniques are utilized. A benchmark is provided to verify the applicability of this study and to demonstrate that the proposed methods can achieve the required aesthetic and counterfeit-proof purposes.
Public viewpoints on new non-invasive prenatal genetic tests
Authors: Farrimond Hannah R. , Kelly Susan E.
Published: November 2011

Prenatal screening programmes have been critiqued for their routine implementation according to clinical rationale without public debate. A new approach, non-invasive prenatal diagnosis (NIPD), promises diagnosis of fetal genetic disorders from a sample of maternal blood without the miscarriage risk of current invasive prenatal tests (e.g. amniocentesis). Little research has investigated the attitudes of wider publics to NIPD. This study used Q-methodology, which combines factor analysis with qualitative comments, to identify four distinct “viewpoints” amongst 71 UK men and women: 1. NIPD as a new tool in the ongoing societal discrimination against the disabled; 2. NIPD as a positive clinical application offering peace of mind in pregnancy; 3. NIPD as a medical option justified for severe disorders only; and 4. NIPD as a valid expansion of personal choice. Concerns included the “trivialisation of testing” and the implications of commercial/direct-to-consumer tests. Q-methodology has considerable potential to identify viewpoints and frame public debate about new technologies.
Abnormal Weight Status and Associated Characteristics of Low-Income Korean Children
Authors: Yoo Seunghyun , Kim Hyekyeong , Lee Jungwha
Published: November 2011

Background. This study reports the prevalence of abnormal weight and associated characteristics of low-income Korean children. Methods Health examination and survey were conducted nationally with 2033 low-income elementary students (mean age ± SD = 9.42 ± 1.65 years). Prevalence of abnormal weight and odds ratios (ORs) of health behaviors for abnormal weight groups were calculated. Results. Prevalence was 8% for underweight and 16% for overweight/obese. Compared with normal weight children, overweight/obese children were more likely to eat a variety of foods (OR = 1.82, P = .04) and have 3 meals a day (OR = 2.07, P = .047), but they were more likely to be stressed by their appearance (OR = 3.88, P < .001). Underweight children were less likely to have night snacks (OR = 0.28, P = .038) and more stressed by academic performance (OR = 3.76, P = .005) but were less educated about physical education (OR = 3.19, P = .044) and substance abuse (OR = 2.78, P = .018). Conclusion Higher prevalence of abnormal weight among low-income Korean children calls for attention to address both childhood obesity and underweight in public health research and practice.
Racial Profiling Litigation
Authors: Withrow Brian L. , Dailey Jeffrey Doug
Published: November 2011

The racial profiling controversy is now about a decade and a half old and has developed into a bona fide research agenda. Hundreds of police departments have conducted studies and scores of researchers have written articles and books on the topic. The controversy came to prominence during a high-profile litigation involving the New Jersey State Police. Since then, with the exception of a few local cases, the courts have been relatively silent at the national level. Even so, a litigation pattern is beginning to emerge. This article documents this pattern and provides evidence that racial profiling litigation is likely to increase in the very near future.
Sarcomatoid Thymic Carcinoma Arising in Metaplastic Thymoma
Authors: Lu Hong-sheng , Gan Mei-fu , Zhou Tao , Wang Shuang-zhu
Published: November 2011

Metaplastic thymoma is an extremely rare tumor. To date, only 17 cases of metaplastic thymoma have been reported. To the best of the authors’ knowledge, this is the second reported case of a sarcomatoid carcinoma arising in metaplastic thymoma; the carcinoma in this case is larger than that in the previous case. A 63-year-old woman with cough and asthenia for 2 weeks was admitted to the hospital. Computed tomography (CT) revealed a giant mass on the right side of the front mediastinum medium. The mediastinal tumor was excised, and additional pathological examinations, immunohistochemical tests, and electron-microscopic tests were performed. The tumor was diagnosed as a sarcomatoid carcinoma arising in metaplastic thymoma. Here, the authors discuss the clinical pathology of the sarcomatoid carcinoma arising in metaplastic thymoma and describe the biological behaviors with respect to the pathological features.
Shared understandings of dementia? An application of the Common Sense Self Regulation Model to a case study
Authors: Glidewell Liz , Johnston Marie , Thomas Ruth
Published: November 2011

This in-depth case study explored whether one health care triad (a person with a diagnosis of dementia (PWD), their caregiver and primary care doctor) spoke about diagnosis in terms of the Common Sense-Self Regulation Model (CS-SRM) (H. Leventhal, et al., 1984 ) Illness Representations (IRs: identity, cause, timeline, consequences and the ability to cure or control the condition), and consider whether PWD IRs are understood by their caregiver and doctor. Each participant was asked a series of open questions, followed by questions prompting for each IR. The caregiver and doctor were interviewed about how PWD thought. All talked about IRs without prompting, with the exception of cause. Prompting for IRs elicited additional data. There were areas where participants shared IRs, but also areas of discrepancy which could have implications for health outcomes. IRs can provide a theoretical framework to elicit how people think about dementia, which could improve shared understandings in clinical practice.
Engagement enacted: Essentials of initiating an action research project
Authors: Snoeren Miranda MWC , Niessen Theo JH , Abma Tineke A
Published: November 2011

Engagement is seen as an important characteristic of action research. The term is often used to refer to the participation and involvement of the research participants. Within this article we take another angle and explore the concept of engagement in relation to the main action researcher. Using an auto-ethnographic approach, we illustrate that the involvement and ‘closeness’ of the researcher, although necessary within action research, can also have a darker side as people have the tendency to get trapped in their own beliefs and prejudices. If not mindful enough of their own involvement and way of being within the context, the researcher can lose him- or herself in the situation and no longer be able to encourage or facilitate the participation of others. We give suggestions for realizing productive engagement as a (participatory) researcher using concepts such as mindfulness and mindsight.
Science, ethics and education
Authors: Elgin Catherine
Published: November 2011

An overarching epistemological goal of science is to develop a comprehensive, systematic, empirically grounded understanding of nature. Two obstacles stand in the way: (1) Nature is enormously complicated. (2) Findings are fallible: no matter how well established a conclusion is, it still might be wrong. To pursue this goal in light of the obstacles, science incorporates ethical values. These values are not mere means; their realization is integral to the sort of understanding that science embodies. The recognition of these values should be incorporated into science education.
Primary Strategy Learning Networks
Authors: Moore Tessa A. , Rutherford Desmond
Published: November 2011

The use of networks as a means of communicating knowledge and ideas and in promoting innovation among schools has emerged globally over the past decade. Currently, inter-school collaboration is not only at the fore nationally in England, but also has become integral to the school improvement agenda. However, networking theory is a disparate field and its application in education is very variable. Nevertheless, there seems to be consistent policy support at government level for school collaborative working arrangements as a key means for promoting the standards agenda. This article explores the effectiveness of one such initiative—Primary Strategy Learning Networks (PSLNs). This is a qualitative study of two PSLNs in England over the course of the first year of the initiative. The research not only seeks to understand the term ‘network’ and what it means methodologically, but also what the implications are of a centrally directed model of networking. The findings of this research offer (1) an alternative model for productive networking and (2) a different perspective to planning for success. These findings will add to the national and international debate on networks as power bases for school improvement.
A genome-wide meta-analysis of nodular sclerosing Hodgkin lymphoma identifies risk loci at 6p21.32
Authors: Cozen Wendy , Li Dalin , Best Timothy , Van Den Berg David J. , Gourraud Pierre-Antoine , Cortessis Victoria K. , Skol Andrew D. , Mack Thomas M. , Glaser Sally L. , Weiss Lawrence M. , Nathwani Bharat N. , Bhatia Smita , Schumacher Fredrick R. , Edlund Christopher K. , Hwang Amie E. , Slager Susan L. , Fredericksen Zachary S. , Strong Louise C. , Habermann Thomas M. , Link Brian K. , Cerhan James R. , Robison Leslie L. , Conti David V. , Onel Kenan
Published: November 2011

Nodular sclerosing Hodgkin lymphoma (NSHL) is a distinct, highly heritable Hodgkin lymphoma subtype. We undertook a genome-wide meta-analysis of 393 European-origin adolescent/young adult NSHL patients and 3315 controls using the Illumina Human610-Quad Beadchip and Affymetrix Genome-Wide Human SNP Array 6.0. We identified 3 single nucleotide polymorphisms (SNPs) on chromosome 6p21.32 that were significantly associated with NSHL risk: rs9268542 (P = 5.35 × 10−10), rs204999 (P = 1.44 × 10−9), and rs2858870 (P = 1.69 × 10−8). We also confirmed a previously reported association in the same region, rs6903608 (P = 3.52 × 10−10). rs204999 and rs2858870 were weakly correlated (r2 = 0.257), and the remaining pairs of SNPs were not correlated (r2 < 0.1). In an independent set of 113 NSHL cases and 214 controls, 2 SNPs were significantly associated with NSHL and a third showed a comparable odds ratio (OR). These SNPs are found on 2 haplotypes associated with NSHL risk (rs204999-rs9268528-rs9268542-rs6903608-rs2858870; AGGCT, OR = 1.7, P = 1.71 × 10−6; GAATC, OR = 0.4, P = 1.16 × 10−4). All individuals with the GAATC haplotype also carried the HLA class II DRB1*0701 allele. In a separate analysis, the DRB1*0701 allele was associated with a decreased risk of NSHL (OR = 0.5, 95% confidence interval = 0.4, 0.7). These data support the importance of the HLA class II region in NSHL etiology.
Human MAIT and CD8αα cells develop from a pool of type-17 precommitted CD8+ T cells
Authors: Walker Lucy J. , Kang Yu-Hoi , Smith Matthew O. , Tharmalingham Hannah , Ramamurthy Narayan , Fleming Vicki M. , Sahgal Natasha , Leslie Alistair , Oo Ye , Geremia Alessandra , Scriba Thomas J. , Hanekom Willem A. , Lauer Georg M. , Lantz Olivier , Adams David H. , Powrie Fiona , Barnes Eleanor , Klenerman Paul
Published: November 2011

Human mucosal associated invariant T (MAIT) CD8+ and Tc17 cells are important tissue-homing cell populations, characterized by high expression of CD161 (++) and type-17 differentiation, but their origins and relationships remain poorly defined. By transcriptional and functional analyses, we demonstrate that a pool of polyclonal, precommitted type-17 CD161++CD8αβ+ T cells exist in cord blood, from which a prominent MAIT cell (TCR Vα7.2+) population emerges post-natally. During this expansion, CD8αα T cells appear exclusively within a CD161++CD8+/MAIT subset, sharing cytokine production, chemokine-receptor expression, TCR-usage, and transcriptional profiles with their CD161++CD8αβ+ counterparts. Our data demonstrate the origin and differentiation pathway of MAIT-cells from a naive type-17 precommitted CD161++CD8+ T-cell pool and the distinct phenotype and function of CD8αα cells in man.
Contrasting dynamic responses in vivo of the Bcl-xL and Bim erythropoietic survival pathways
Authors: Koulnis Miroslav , Porpiglia Ermelinda , Porpiglia P. Alberto , Liu Ying , Hallstrom Kelly , Hidalgo Daniel , Socolovsky Merav
Published: November 2011

Survival signaling by the erythropoietin (Epo) receptor (EpoR) is essential for erythropoiesis and for its acceleration in hypoxic stress. Several apparently redundant EpoR survival pathways were identified in vitro, raising the possibility of their functional specialization in vivo. Here we used mouse models of acute and chronic stress, including a hypoxic environment and β-thalassemia, to identify two markedly different response dynamics for two erythroblast survival pathways in vivo. Induction of the antiapoptotic protein Bcl-xL is rapid but transient, while suppression of the proapoptotic protein Bim is slower but persistent. Similar to sensory adaptation, however, the Bcl-xL pathway “resets,” allowing it to respond afresh to acute stress superimposed on a chronic stress stimulus. Using “knock-in” mouse models expressing mutant EpoRs, we found that adaptation in the Bcl-xL response occurs because of adaptation of its upstream regulator Stat5, both requiring the EpoR distal cytoplasmic domain. We conclude that survival pathways show previously unsuspected functional specialization for the acute and chronic phases of the stress response. Bcl-xL induction provides a “stop-gap” in acute stress, until slower but permanent pathways are activated. Furthermore, pathologic elevation of Bcl-xL may be the result of impaired adaptation, with implications for myeloproliferative disease mechanisms.
Prediction using step-wise L1, L2 regularization and feature selection for small data sets with large number of features
Authors: Demir-Kavuk Ozgur , Kamada Mayumi , Akutsu Tatsuya , Knapp Ernst-Walter
Published: November 2011

Background

Machine learning methods are nowadays used for many biological prediction problems involving drugs, ligands or polypeptide segments of a protein. In order to build a prediction model a so called training data set of molecules with measured target properties is needed. For many such problems the size of the training data set is limited as measurements have to be performed in a wet lab. Furthermore, the considered problems are often complex, such that it is not clear which molecular descriptors (features) may be suitable to establish a strong correlation with the target property. In many applications all available descriptors are used. This can lead to difficult machine learning problems, when thousands of descriptors are considered and only few (e.g. below hundred) molecules are available for training.

Results

The CoEPrA contest provides four data sets, which are typical for biological regression problems (few molecules in the training data set and thousands of descriptors). We applied the same two-step training procedure for all four regression tasks. In the first stage, we used optimized L1 regularization to select the most relevant features. Thus, the initial set of more than 6,000 features was reduced to about 50. In the second stage, we used only the selected features from the preceding stage applying a milder L2 regularization, which generally yielded further improvement of prediction performance. Our linear model employed a soft loss function which minimizes the influence of outliers.

Conclusions

The proposed two-step method showed good results on all four CoEPrA regression tasks. Thus, it may be useful for many other biological prediction problems where for training only a small number of molecules are available, which are described by thousands of descriptors.
A pair of orthologs of a leucine-rich repeat receptor kinase-like disease resistance gene family regulates rice response to raised temperature
Authors: Zhang Haitao , Cao Yinglong , Zhao Jing , Li Xianghua , Xiao Jinghua , Wang Shiping
Published: November 2011

Background

Rice Xa3/Xa26 disease-resistance gene encodes a leucine-rich repeat (LRR) receptor kinase-type protein against Xanthomonas oryzae pv. oryzae (Xoo) and belongs to a multigene family. However, the functions of most genes in this family are unknown.

Results

Here we report that two orthologs of this family, the NRKe from rice variety Nipponbare and 9RKe from variety 93-11 at the RKe locus, have similar functions although they encode different proteins. This pair of orthologs could not mediate resistance to Xoo, but they were transcriptionally induced by raised temperature. Transcriptional activation of NRKe or 9RKe resulted in the formation of temperature-sensitive lesion mimics, which were spots of dead cells associated with accumulation of superoxides, in different organs of the transgenic plants. These plants were more sensitive to high temperature shock than wild-type controls. Transgenic plants carrying a chimeric protein consisting of the LRR domain of NRKe and the kinase domain of Xa3/Xa26 developed the same lesion mimics as the NRKe-transgenic plants, whereas transgenic plants carrying another chimeric protein consisting of the LRR domain of Xa3/Xa26 and the kinase domain of NRKe were free of lesion mimic. All the transgenic plants carrying a chimeric protein were susceptible to Xoo.

Conclusion

These results suggest that the RKe locus is involved in rice response to raised temperature. The LRR domain of RKe protein appears to be important to sense increased temperature. The RKe-involved temperature-related pathway and Xa3/Xa26-mediated disease-resistance pathway may partially overlap.
Inputs to quality: supervision, management, and community involvement in health facilities in Egypt in 2004
Authors: Cherlin Emily J , Allam Adel A , Linnander Erika L , Wong Rex , El-Toukhy Essam , Sipsma Heather , Krumholz Harlan M , Curry Leslie A , Bradley Elizabeth H
Published: November 2011

Background

As low- and middle-income countries experience economic development, ensuring quality of health care delivery is a central component of health reform. Nevertheless, health reforms in low- and middle-income countries have focused more on access to services rather than the quality of these services, and reporting on quality has been limited. In the present study, we sought to examine the prevalence and regional variation in key management practices in Egyptian health facilities within three domains: supervision of the facility from the Ministry of Health and Population (MOHP), managerial processes, and patient and community involvement in care.

Methods

We conducted a cross-sectional analysis of data from 559 facilities surveyed with the Egyptian Service Provision Assessment (ESPA) survey in 2004, the most recent such survey in Egypt. We registered on the Measure Demographic and Health Survey (DHS) website http://legacy.measuredhs.com/login.cfm to gain access to the survey data. From the ESPA sampled 559 MOHP facilities, we excluded a total of 79 facilities because they did not offer facility-based 24-hour care or have at least one physician working in the facility, resulting in a final sample of 480 facilities. The final sample included 76 general service hospitals, 307 rural health units, and 97 maternal and child health and urban health units (MCH/urban units). We used standard frequency analyses to describe facility characteristics and tested the statistical significance of regional differences using chi-square statistics.

Results

Nearly all facilities reported having external supervision within the 6 months preceding the interview. In contrast, key facility-level managerial processes, such as having routine and documented management meetings and applying quality assurance approaches, were uncommon. Involvement of communities and patients was also reported in a minority of facilities. Hospitals and health units located in Urban Egypt compared with more rural parts of Egypt were significantly more likely to have management committees that met at least monthly, to keep official records of the meetings, and to have an approach for reviewing quality assurance activities.

Conclusions

Although the data precede the recent reform efforts of the MOHP, they provide a baseline against which future progress can be measured. Targeted efforts to improve facility-level management are critical to supporting quality improvement initiatives directed at improving the quality of health care throughout the country.
Effects of granulocyte-colony stimulating factor (G-CSF) on diabetic cardiomyopathy in Otsuka Long-Evans Tokushima Fatty rats
Authors: Lim Young-Hyo , Joe Jun-Ho , Jang Ki-Seok , Song Yi-Sun , So Byung-Im , Fang Cheng-Hu , Shin Jinho , Kim Jung-Hyun , Lim Heon-Kil , Kim Kyung-Soo
Published: November 2011

Background

Diabetic cardiomyopathy (CMP) is a common and disabling disease in diabetic patients, however no effective treatments have been developed. Although granulocyte-colony stimulating factor (G-CSF) improves heart function in myocardial infarction, its effect on non-ischemic CMP such as diabetic CMP is unknown. In the present study, we investigated the effects of G-CSF on diabetic CMP in a rat model of type II diabetes.

Methods

Twenty 7-week-old male Otsuka Long-Evans Tokushima Fatty (OLETF: a rat model of diabetes) rats and 10 male Long-Evans Tokushima Otsuka (LETO: normal controls) rats were used. All of the LETO and 8 OLETF rats were fed on tap water while the rest were fed on sucrose-containing water. After 10 weeks, saline or recombinant human G-CSF (100 μg/kg/day) was injected intraperitoneally for 5 days. Blood levels of glucose, total cholesterol and triglyceride, and Doppler echocardiograms for diastolic dysfunction were obtained just before and 4 weeks after the saline or G-CSF treatment. Light microscopy, electron microscopy (EM) and immunohistochemistry for transforming growth factor-β were employed to examine myocardial histology 4 weeks after the saline or G-CSF treatment.

Results

Diastolic dysfunction developed at 17 weeks (before the saline or G-CSF treatment) in the OLETF rats whether or not they were fed sucrose water, but were more severe in those fed sucrose water. Four weeks after saline or G-CSF treatment, diastolic function had recovered in the G-CSF-treated group regardless of sucrose water feeding, and perivascular and/or interstitial fibrosis in the G-CSF-treated group had decreased significantly. TGF-β immunoreactivity in the interstitial and perivascular tissue was also reduced in the G-CSF-treated group, and EM studies revealed less severe disruption of myofilaments and mitochondrial cristae, and decreased collagen deposition.

Conclusions

G-CSF can ameliorate cardiac diastolic dysfunction and morphological damage, especially fibrosis of the myocardium, in OLETF rats with diabetic CMP.
Heterologous protein display on the cell surface of lactic acid bacteria mediated by the s-layer protein
Authors: Hu Shumin , Kong Jian , Sun Zhilan , Han Lanlan , Kong Wentao , Yang Pu
Published: November 2011

Background

Previous studies have revealed that the C-terminal region of the S-layer protein from Lactobacillus is responsible for the cell wall anchoring, which provide an approach for targeting heterologous proteins to the cell wall of lactic acid bacteria (LAB). In this study, we developed a new surface display system in lactic acid bacteria with the C-terminal region of S-layer protein SlpB of Lactobacillus crispatus K2-4-3 isolated from chicken intestine.

Results

Multiple sequence alignment revealed that the C-terminal region (LcsB) of Lb. crispatus K2-4-3 SlpB had a high similarity with the cell wall binding domains SA and CbsA of Lactobacillus acidophilus and Lb. crispatus. To evaluate the potential application as an anchoring protein, the green fluorescent protein (GFP) or beta-galactosidase (Gal) was fused to the N-terminus of the LcsB region, and the fused proteins were successfully produced in Escherichia coli, respectively. After mixing them with the non-genetically modified lactic acid bacteria cells, the fused GFP-LcsB and Gal-LcsB were functionally associated with the cell surface of various lactic acid bacteria tested. In addition, the binding capacity could be improved by SDS pretreatment. Moreover, both of the fused proteins could simultaneously bind to the surface of a single cell. Furthermore, when the fused DNA fragment of gfp:lcsB was inserted into the Lactococcus lactis expression vector pSec:Leiss:Nuc, the GFP could not be secreted into the medium under the control of the nisA promoter. Western blot, in-gel fluorescence assay, immunofluorescence microscopy and SDS sensitivity analysis confirmed that the GFP was successfully expressed onto the cell surface of L. lactis with the aid of the LcsB anchor.

Conclusion

The LcsB region can be used as a functional scaffold to target the heterologous proteins to the cell surfaces of lactic acid bacteria in vitro and in vivo, and has also the potential for biotechnological application.
Automatic discrimination between safe and unsafe swallowing using a reputation-based classifier
Authors: Nikjoo Mohammad S , Steele Catriona M , Sejdić Ervin , Chau Tom
Published: November 2011

Background

Swallowing accelerometry has been suggested as a potential non-invasive tool for bedside dysphagia screening. Various vibratory signal features and complementary measurement modalities have been put forth in the literature for the potential discrimination between safe and unsafe swallowing. To date, automatic classification of swallowing accelerometry has exclusively involved a single-axis of vibration although a second axis is known to contain additional information about the nature of the swallow. Furthermore, the only published attempt at automatic classification in adult patients has been based on a small sample of swallowing vibrations.

Methods

In this paper, a large corpus of dual-axis accelerometric signals were collected from 30 older adults (aged 65.47 ± 13.4 years, 15 male) referred to videofluoroscopic examination on the suspicion of dysphagia. We invoked a reputation-based classifier combination to automatically categorize the dual-axis accelerometric signals into safe and unsafe swallows, as labeled via videofluoroscopic review. From these participants, a total of 224 swallowing samples were obtained, 164 of which were labeled as unsafe swallows (swallows where the bolus entered the airway) and 60 as safe swallows. Three separate support vector machine (SVM) classifiers and eight different features were selected for classification.

Results

With selected time, frequency and information theoretic features, the reputation-based algorithm distinguished between safe and unsafe swallowing with promising accuracy (80.48 ± 5.0%), high sensitivity (97.1 ± 2%) and modest specificity (64 ± 8.8%). Interpretation of the most discriminatory features revealed that in general, unsafe swallows had lower mean vibration amplitude and faster autocorrelation decay, suggestive of decreased hyoid excursion and compromised coordination, respectively. Further, owing to its performance-based weighting of component classifiers, the static reputation-based algorithm outperformed the democratic majority voting algorithm on this clinical data set.

Conclusion

Given its computational efficiency and high sensitivity, reputation-based classification of dual-axis accelerometry ought to be considered in future developments of a point-of-care swallow assessment where clinical informatics are desired.
The effect of cocoa supplementation on hepatic steatosis, reactive oxygen species and LFABP in a rat model of NASH
Authors: Janevski Mile , Antonas Kiriakos N , Sullivan-Gunn Melanie J , McGlynn Maree A , Lewandowski Paul A
Published: November 2011

Background

Non alcoholic steatohepatitis is hypothesised to develop via a mechanism involving fat accumulation and oxidative stress. The current study aimed to investigate if an increase in oxidative stress was associated with changes in the expression of liver fatty acid binding protein in a rat model of non alcoholic steatohepatitis and whether cocoa supplementation attenuated those changes.

Methods

Female Sprague Dawley rats were fed a high fat control diet, a high fat methionine choline deficient diet, or one of four 12.5% cocoa supplementation regimes in combination with the high fat methionine choline deficient diet.

Results

Liver fatty acid binding protein mRNA and protein levels were reduced in the liver of animals with fatty liver disease when compared to controls. Increased hepatic fat content was accompanied by higher levels of oxidative stress in animals with fatty liver disease when compared to controls. An inverse association was found between the levels of hepatic liver fatty acid binding protein and the level of hepatic oxidative stress in fatty liver disease. Elevated NADPH oxidase protein levels were detected in the liver of animals with increased severity in inflammation and fibrosis. Cocoa supplementation was associated with partial attenuation of these pathological changes, although the severity of liver disease induced by the methionine choline deficient diet prevented complete reversal of any disease associated changes. Red blood cell glutathione was increased by cocoa supplementation, whereas liver glutathione was reduced by cocoa compared to methionine choline deficient diet fed animals.

Conclusion

These findings suggest a potential role for liver fatty acid binding protein and NADPH oxidase in the development of non alcoholic steatohepatitis. Furthermore, cocoa supplementation may have be of therapeutic benefit in less sever forms of NASH.
A proteomic approach to analyzing responses of Arabidopsis thaliana root cells to different gravitational conditions using an agravitropic mutant, pin2 and its wild type
Authors: Tan Chao , Wang Hui , Zhang Yue , Qi Bin , Xu Guoxin , Zheng Huiqiong
Published: November 2011

Background

Root gravitropsim has been proposed to require the coordinated, redistribution of the plant signaling molecule auxin within the root meristem, but the underlying molecular mechanisms are still unknown. PIN proteins are membrane transporters that mediate the efflux of auxin from cells. The PIN2 is important for the basipetal transport of auxin in roots and plays a critical role in the transmission of gravity signals perceived in the root cap to the root elongation zone. The loss of function pin2 mutant exhibits a gravity-insensitive root growth phenotype. By comparing the proteomes of wild type and the pin2 mutant root tips under different gravitational conditions, we hope to identify proteins involved in the gravity-related signal transduction.

Results

To identify novel proteins involved in the gravity signal transduction pathway we have carried out a comparative proteomic analysis of Arabidopsis pin2 mutant and wild type (WT) roots subjected to different gravitational conditions. These conditions included horizontal (H) and vertical (V) clinorotation, hypergravity (G) and the stationary control (S). Analysis of silver-stained two-dimensional SDS-PAGE gels revealed 28 protein spots that showed significant expression changes in altered gravity (H or G) compared to control roots (V and S). Whereas the majority of these proteins exhibited similar expression patterns in WT and pin2 roots, a significant number displayed different patterns of response between WT and pin2 roots. The latter group included 11 protein spots in the H samples and two protein spots in the G samples that exhibited an altered expression exclusively in WT but not in pin2 roots. One of these proteins was identified as annexin2, which was induced in the root cap columella cells under altered gravitational conditions.

Conclusions

The most interesting observation in this study is that distinctly different patterns of protein expression were found in WT and pin 2 mutant roots subjected to altered gravity conditions. The data also demonstrate that PIN2 mutation not only affects the basipetal transport of auxin to the elongation zone, but also results in an altered expression of proteins in the root columella.
Validity of the international physical activity questionnaire short form (IPAQ-SF): A systematic review
Authors: Lee Paul H , Macfarlane Duncan J , Lam TH , Stewart Sunita M
Published: November 2011

Background

The International Physical Activity Questionnaire - Short Form (IPAQ-SF) has been recommended as a cost-effective method to assess physical activity. Several studies validating the IPAQ-SF have been conducted with differing results, but no systematic review of these studies has been reported.

Methods

The keywords "IPAQ", "validation", and "validity" were searched in PubMed and Scopus. Studies published in English that validated the IPAQ-SF against an objective physical activity measuring device, doubly labeled water, or an objective fitness measure were included.

Results

Twenty-three validation studies were included in this review. There was a great deal of variability in the methods used across studies, but the results were largely similar. Correlations between the total physical activity level measured by the IPAQ-SF and objective standards ranged from 0.09 to 0.39; none reached the minimal acceptable standard in the literature (0.50 for objective activity measuring devices, 0.40 for fitness measures). Correlations between sections of the IPAQ-SF for vigorous activity or moderate activity level/walking and an objective standard showed even greater variability (-0.18 to 0.76), yet several reached the minimal acceptable standard. Only six studies provided comparisons between physical activity levels derived from the IPAQ-SF and those obtained from objective criterion. In most studies the IPAQ-SF overestimated physical activity level by 36 to 173 percent; one study underestimated by 28 percent.

Conclusions

The correlation between the IPAQ-SF and objective measures of activity or fitness in the large majority of studies was lower than the acceptable standard. Furthermore, the IPAQ-SF typically overestimated physical activity as measured by objective criterion by an average of 84 percent. Hence, the evidence to support the use of the IPAQ-SF as an indicator of relative or absolute physical activity is weak.
Fabrication of superhydrophobic and antibacterial surface on cotton fabric by doped silica- based sols with nanoparticles of copper
Authors: Berendjchi Amirhosein , Khajavi Ramin , Yazdanshenas Mohammad Esmaeil
Published: November 2011

The study discussed the synthesis of silica sol using the sol-gel method, doped with two different amounts of Cu nanoparticles. Cotton fabric samples were impregnated by the prepared sols and then dried and cured. To block hydroxyl groups, some samples were also treated with hexadecyltrimethoxysilane. The average particle size of colloidal silica nanoparticles were measured by the particle size analyzer. The morphology, roughness, and hydrophobic properties of the surface fabricated on cotton samples were analyzed and compared via the scanning electron microscopy, the transmission electron microscopy, the scanning probe microscopy, with static water contact angle (SWC), and water shedding angle measurements. Furthermore, the antibacterial efficiency of samples was quantitatively evaluated using AATCC 100 method. The addition of 0.5% (wt/wt) Cu into silica sol caused the silica nanoparticles to agglomerate in more grape-like clusters on cotton fabrics. Such fabricated surface revealed the highest value of SWC (155° for a 10-μl droplet) due to air trapping capability of its inclined structure. However, the presence of higher amounts of Cu nanoparticles (2% wt/wt) in silica sol resulted in the most slippery smooth surface on cotton fabrics. All fabricated surfaces containing Cu nanoparticles showed the perfect antibacterial activity against both of gram-negative and gram-positive bacteria.
Oral administration of the KATP channel opener diazoxide ameliorates disease progression in a murine model of multiple sclerosis
Authors: Virgili Noemí , Espinosa-Parrilla Juan F , Mancera Pilar , Pastén-Zamorano Andrea , Gimeno-Bayon Javier , Rodríguez Manuel J , Mahy Nicole , Pugliese Marco
Published: November 2011

Background

Multiple Sclerosis (MS) is an acquired inflammatory demyelinating disorder of the central nervous system (CNS) and is the leading cause of nontraumatic disability among young adults. Activated microglial cells are important effectors of demyelination and neurodegeneration, by secreting cytokines and others neurotoxic agents. Previous studies have demonstrated that microglia expresses ATP-sensitive potassium (KATP) channels and its pharmacological activation can provide neuroprotective and anti-inflammatory effects. In this study, we have examined the effect of oral administration of KATP channel opener diazoxide on induced experimental autoimmune encephalomyelitis (EAE), a mouse model of MS.

Methods

Anti-inflammatory effects of diazoxide were studied on lipopolysaccharide (LPS) and interferon gamma (IFNγ)-activated microglial cells. EAE was induced in C57BL/6J mice by immunization with myelin oligodendrocyte glycoprotein peptide (MOG35-55). Mice were orally treated daily with diazoxide or vehicle for 15 days from the day of EAE symptom onset. Treatment starting at the same time as immunization was also assayed. Clinical signs of EAE were monitored and histological studies were performed to analyze tissue damage, demyelination, glial reactivity, axonal loss, neuronal preservation and lymphocyte infiltration.

Results

Diazoxide inhibited in vitro nitric oxide (NO), tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6) production and inducible nitric oxide synthase (iNOS) expression by activated microglia without affecting cyclooxygenase-2 (COX-2) expression and phagocytosis. Oral treatment of mice with diazoxide ameliorated EAE clinical signs but did not prevent disease. Histological analysis demonstrated that diazoxide elicited a significant reduction in myelin and axonal loss accompanied by a decrease in glial activation and neuronal damage. Diazoxide did not affect the number of infiltrating lymphocytes positive for CD3 and CD20 in the spinal cord.

Conclusion

Taken together, these results demonstrate novel actions of diazoxide as an anti-inflammatory agent, which might contribute to its beneficial effects on EAE through neuroprotection. Treatment with this widely used and well-tolerated drug may be a useful therapeutic intervention in ameliorating MS disease.
A whole genome screen for HIV restriction factors
Authors: Liu Li , Oliveira Nidia MM , Cheney Kelly M , Pade Corinna , Dreja Hanna , Bergin Ann-Marie H , Borgdorff Viola , Beach David H , Bishop Cleo L , Dittmar Matthias T , McKnight Áine
Published: November 2011

Background

Upon cellular entry retroviruses must avoid innate restriction factors produced by the host cell. For human immunodeficiency virus (HIV) human restriction factors, APOBEC3 (apolipoprotein-B-mRNA-editing-enzyme), p21 and tetherin are well characterised.

Results

To identify intrinsic resistance factors to HIV-1 replication we screened 19,121 human genes and identified 114 factors with significant inhibition of infection. Those with a known function are involved in a broad spectrum of cellular processes including receptor signalling, vesicle trafficking, transcription, apoptosis, cross-nuclear membrane transport, meiosis, DNA damage repair, ubiquitination and RNA processing. We focused on the PAF1 complex which has been previously implicated in gene transcription, cell cycle control and mRNA surveillance. Knockdown of all members of the PAF1 family of proteins enhanced HIV-1 reverse transcription and integration of provirus. Over-expression of PAF1 in host cells renders them refractory to HIV-1. Simian Immunodeficiency Viruses and HIV-2 are also restricted in PAF1 expressing cells. PAF1 is expressed in primary monocytes, macrophages and T-lymphocytes and we demonstrate strong activity in MonoMac1, a monocyte cell line.

Conclusions

We propose that the PAF1c establishes an anti-viral state to prevent infection by incoming retroviruses. This previously unrecognised mechanism of restriction could have implications for invasion of cells by any pathogen.
Expression of mutant TDP-43 induces neuronal dysfunction in transgenic mice
Authors: Xu Ya-Fei , Zhang Yong-Jie , Lin Wen-Lang , Cao Xiangkun , Stetler Caroline , Dickson Dennis W , Lewis Jada , Petrucelli Leonard
Published: November 2011

Background

Abnormal distribution, modification and aggregation of transactivation response DNA-binding protein 43 (TDP-43) are the hallmarks of multiple neurodegenerative diseases, especially frontotemporal lobar degeneration with ubiquitin-positive inclusions (FTLD-U) and amyotrophic lateral sclerosis (ALS). Researchers have identified 44 mutations in the TARDBP gene that encode TDP-43 as causative for cases of sporadic and familial ALS http://www.molgen.ua.ac.be/FTDMutations/. Certain mutant forms of TDP-43, such as M337V, are associated with increased low molecular weight (LMW) fragments compared to wild-type (WT) TDP-43 and cause neuronal apoptosis and developmental delay in chick embryos. Such findings support a direct link between altered TDP-43 function and neurodegeneration.

Results

To explore the pathogenic properties of the M337V mutation, we generated and characterized two mouse lines expressing human TDP-43 (hTDP-43M337V) carrying this mutation. hTDP-43M337V was expressed primarily in the nuclei of neurons in the brain and spinal cord, and intranuclear and cytoplasmic phosphorylated TDP-43 aggregates were frequently detected. The levels of TDP-43 LMW products of ~25 kDa and ~35 kDa species were also increased in the transgenic mice. Moreover, overexpression of hTDP-43M337V dramatically down regulated the levels of mouse TDP-43 (mTDP-43) protein and RNA, indicating TDP-43 levels are tightly controlled in mammalian systems. TDP-43M337V mice displayed reactive gliosis, widespread ubiquitination, chromatolysis, gait abnormalities, and early lethality. Abnormal cytoplasmic mitochondrial aggregates and abnormal phosphorylated tau were also detected in the mice.

Conclusion

Our novel TDP-43M337V mouse model indicates that overexpression of hTDP-43M337V alone is toxic in vivo. Because overexpression of hTDP-43 in wild-type TDP-43 and TDP-43M337V mouse models produces similar phenotypes, the mechanisms causing pathogenesis in the mutant model remain unknown. However, our results suggest that overexpression of the hTDP-43M337V can cause neuronal dysfunction due to its effect on a number of cell organelles and proteins, such as mitochondria and TDP-43, that are critical for neuronal activity. The mutant model will serve as a valuable tool in the development of future studies designed to uncover pathways associated with TDP-43 neurotoxicity and the precise roles TDP-43 RNA targets play in neurodegeneration.