bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparison of Primers for the Detection of Phytophthora (and Other Oomycetes) from Environmental Samples
Authors: Burgess Treena I. , White Diane , Sapsford Sarah J.
Published: September 2022

Many oomycetes are important plant pathogens that cause devastating diseases in agricultural fields, orchards, urban areas, and natural ecosystems. Limitations and difficulties associated with isolating these pathogens have led to a strong uptake of DNA metabarcoding and mass parallel sequencing. At least 21 primer combinations have been designed to amplify oomycetes, or more specifically, Phytophthora species, from environmental samples. We used the Illumina sequencing platform to compare 13 primer combinations on mock communities and environmental samples. The primer combinations tested varied significantly in their ability to amplify Phytophthora species in a mock community and from environmental samples; this was due to either low sensitivity (unable to detect species present in low concentrations) or a lack of specificity (an inability to amplify some species even if they were present in high concentrations). Primers designed for oomycetes underestimated the Phytophthora community compared to Phytophthora-specific primers. We recommend using technical replicates, primer combinations, internal controls, and a phylogenetic approach for assigning a species identity to OTUs or ASVs. Particular care must be taken if sampling substrates where hybrid species could be expected. Overall, the choice of primers should depend upon the hypothesis being tested.
50 Years of Cumulative Open-Source Data Confirm Stable and Robust Biodiversity Distribution Patterns for Macrofungi
Authors: Yu Haili , Wang Tiejun , Skidmore Andrew , Heurich Marco , Bässler Claus
Published: September 2022

Fungi are a hyper-diverse kingdom that contributes significantly to the regulation of the global carbon and nutrient cycle. However, our understanding of the distribution of fungal diversity is often hindered by a lack of data, especially on a large spatial scale. Open biodiversity data may provide a solution, but concerns about the potential spatial and temporal bias in species occurrence data arising from different observers and sampling protocols challenge their utility. The theory of species accumulation curves predicts that the cumulative number of species reaches an asymptote when the sampling effort is sufficiently large. Thus, we hypothesize that open biodiversity data could be used to reveal large-scale macrofungal diversity patterns if these datasets are accumulated long enough. Here, we tested our hypothesis with 50 years of macrofungal occurrence records in Norway and Sweden that were downloaded from the Global Biodiversity Information Facility (GBIF). We first grouped the data into five temporal subsamples with different cumulative sampling efforts (i.e., accumulation of data for 10, 20, 30, 40 and 50 years). We then predicted the macrofungal diversity and distribution at each subsample using the maximum entropy (MaxEnt) species distribution model. The results revealed that the cumulative number of macrofungal species stabilized into distinct distribution patterns with localized hotspots of predicted macrofungal diversity with sampling efforts greater than approximately 30 years. Our research demonstrates the utility and importance of the long-term accumulated open biodiversity data in studying macrofungal diversity and distribution at the national level.
Prepectoral vs. Submuscular Immediate Breast Reconstruction in Patients Undergoing Mastectomy after Neoadjuvant Chemotherapy: Our Early Experience
Authors: Scardina Lorenzo , Di Leone Alba , Biondi Ersilia , Carnassale Beatrice , Sanchez Alejandro Martin , D’Archi Sabatino , Franco Antonio , Moschella Francesca , Magno Stefano , Terribile Daniela , Gentile Damiano , Fabi Alessandra , D’Angelo Anna , Barone Adesi Liliana , Visconti Giuseppe , Salgarello Marzia , Masetti Riccardo , Franceschini Gianluca
Published: September 2022

Background: Conservative mastectomy with immediate prosthetic breast reconstruction (IPBR) is an oncologically accepted technique that offers improved esthetic results and patient quality of life. Traditionally, implants have been placed in a submuscular (SM) plane beneath the pectoralis major muscle (PMM). Recently, prepectoral (PP) placement of the prosthesis has been increasingly used in order to avoid morbidities related to manipulation of the PMM. The aim of this study was to compare outcomes of SM vs. PP IPBR after conservative mastectomy in patients with histologically proven breast cancer treated with neoadjuvant chemotherapy (NAC). Methods: In this retrospective observational study, we analyzed two cohorts of patients that underwent mastectomy with IPBR after NAC in our institution from January 2018 to December 2021. Conservative mastectomy was performed in 146 of the 400 patients that underwent NAC during the study period. Patients were divided into two groups based on the positioning of implants: 56 SM versus 90 PP. Results: The two cohorts were similar for age (mean age 42 and 44 years in the SM and PP group respectively) and follow-up (33 and 20 months, respectively). Mean operative time was 56 min shorter in the PP group (300 and 244 min in the SM and PP group). No significant differences were observed in overall major complication rates. Implant loss was observed in 1.78% of patients (1/56) in the SM group and 1.11% of patients (1/90) in PP group. No differences were observed between the two groups in local or regional recurrence. Conclusions: Our preliminary experience, which represents one of the largest series of patients undergoing PP-IPBR after NAC at a single institution documented in the literature, seems to confirm that PP-IPBR after NAC is a safe, reliable and effective alternative to traditional SM-IPBR with excellent esthetic and oncological outcomes; it is easy to perform, reduces operative time and minimizes complications related to manipulation of PPM. However, this promising results need to be confirmed in prospective trials with longer follow-up.
Adequacy of Disease Control by Supraomohyoid Neck Dissection in cT1/T2 Tongue Cancer
Authors: Iandelli Andrea , Marchi Filippo , Chen An-Chieh , Young Chi-Kuan , Liao Chun-Ta , Tsao Chung-Kan , Kang Chung-Jan , Wang Hung-Ming , Chang Tung-Chieh Joseph , Huang Shiang-Fu
Published: September 2022

Background: Patients affected by oral tongue squamous cell carcinoma (OTSCC) underwent a supraomohyoid neck dissection (SOHND) or modified radical neck dissection (mRND) according to the clinical nodal status (cN0 vs. cN+). We investigate whether the type of neck dissection affects survival with the presence of extranodal extension (ENE) and multiple nodal metastases (MNM). Methods: We conducted a retrospective study enrolling surgically treated patients affected by cT1/T2 OTSCC and MNM or ENE. The outcomes assessed were: overall survival (OS), disease-free survival (DFS), and neck-control- and metastases-free survival (NC-MFS). Survival curves were plotted by the Kaplan–Meier method and the log-rank test. Furthermore, we conducted a multivariable analysis with the Cox regression model. Results: We included a total of 565 patients (36% cT1, 64% cT2). Of these, 501 patients underwent a SOHND, and 64 underwent an mRND. A total of 184 patients presented rpN+, with 28.7% of these in the SOHND group and 62.5% of these in the mRND group. We identified no significant differences in OS, DFS, and NC-MFS in the whole pN+ cohort, in the MNM, and the ENE subgroups. In the multivariable analysis, the type of ND did not affect OS and DFS. Conclusions: Treating cT1-2 N0/+ tongue cancer with SOHND is oncologically safe. ENE and MNM patients do not benefit from an mRND.
Effects of Oral Supplementation with Myo-Inositol and D-Chiro-Inositol on Ovarian Functions in Female Long-Term Survivors of Lymphoma: Results from a Prospective Case–Control Analysis
Authors: Dellino Miriam , Cascardi Eliano , Leoni Claudia , Fortunato Francesca , Fusco Annarita , Tinelli Raffaele , Cazzato Gerardo , Scacco Salvatore , Gnoni Antonio , Scilimati Antonio , Loizzi Vera , Malvasi Antonio , Sapino Anna , Pinto Vincenzo , Cicinelli Ettore , Di Vagno Giovanni , Cormio Gennaro , Chiantera Vito , Laganà Antonio Simone
Published: September 2022

The progressive improvement of lymphoma treatment has led to an important prolongation of patient survival and life expectancy. The principal international scientific societies of oncology now therefore recommend that long-term survivors of lymphoma join fertility programs. Specifically, fertile-age patients should be assisted by a multidisciplinary team, including specialists dedicated to fertility preservation in oncology, in order to support the completion of their reproductive project. In the general population, the use of Myo-Inositol and D-Chiro-Inositol (MI/DCI) has been demonstrated to be an effective choice to treat ovarian dysfunctions, with a consequent improvement in reproductive outcomes, so it may represent an adjuvant strategy for this purpose. We therefore conducted a pilot prospective case–control study to evaluate the potentialities of this nutritional supplement, with the aim of optimizing reproductive function in female long-term survivors of lymphoma. One group underwent oral supplementation with MI 1200 mg and DCI 135 mg per day for 12 months, compared with controls who underwent no treatment in the same period. After 12 months, FSH, LH, and progesterone levels, as well as oligomenorrhea and antral follicle count (AFC), were significantly improved in the MI/DCI group. In addition, a significantly higher mean value in FSH and LH and a significantly lower mean AFC value in the right ovary were observed in controls compared to the MI/DCI group. Despite the need for further investigation, MI/DCI could be considered a potential adjuvant strategy to restore ovarian function in female long-term survivors of lymphoma.
A Meta-Analysis on the Effectiveness of Sertaconazole 2% Cream Compared with Other Topical Therapies for Seborrheic Dermatitis
Authors: Georgescu Simona , Mitran Cristina , Mitran Madalina , Amuzescu Andreea , Matei Clara , Tampa Mircea
Published: September 2022

Seborrheic dermatitis (SD) is a relapsing inflammatory skin disorder that affects the seborrheic areas of the body. Its etiology is not completely elucidated; however, the link between disease exacerbations and the proliferation of Malassezia spp., along with the good response to antifungal agents, indicate the role of fungi in its pathophysiology. Sertaconazole nitrate is a relatively new imidazole antifungal agent with a particular structure, consisting in a benzothiophene ring similar to the indole ring of tryptophan, and it acts mainly through the inhibition of ergosterol synthesis and the formation of pores in the fungal cell membrane. The aim of our study was to evaluate the efficiency of sertaconazole 2% cream compared with other topical treatments in patients with SD. We performed an extensive literature search by browsing the PubMed database with the keyword combination “sertaconazole AND seborrheic dermatitis AND clinical trial”, which retrieved eight controlled clinical trials evaluating the effects of sertaconazole in SD. All of the clinical trials included a standard scoring index (SI). At 28 days since the beginning of the treatment, the sertaconazole regimen was associated with a significantly higher percentage of patients with mild SI and a lower percentage of patients with moderate or severe SI (odds ratio 0.51) than the other investigated treatments—hydrocortisone, ketoconazole, clotrimazole, metronidazole, pimecrolimus, and tacrolimus (odds ratio 1.95). In conclusion, treatment with sertaconazole 2% cream may represent an efficient alternative therapy for patients with SD.
Ethnicity and Tone Production on Singlish Particles
Authors: Soh Ying Qi , Lee Junwen , Tan Ying-Ying
Published: September 2022

Recent research on Singlish, also known as Colloquial Singapore English, suggests that it is subject to ethnic variation across the three major ethnic groups in Singapore, namely Chinese, Malay, and Indian. Discourse particles, said to be one of the most distinctive features of the language, are nevertheless commonly used by bilinguals across all three ethnic groups. This study seeks to determine whether there are ethnic differences in the pitch contours of Singlish discourse particles produced by Singlish speakers. Four hundred and forty-four tokens of three Singlish particles, sia24, meh55, and what21, produced by the three groups of English-speaking bilingual speakers were drawn from the National Speech Corpus, and the pitch contours extracted and normalized. Statistical analysis of the overall pitch contours, the three acoustic parameters of mean pitch, pitch range, and pitch movement, and the variability of these parameters showed that the effect of ethnicity on the three acoustic parameters was not statistically significant and that the pitch contours of each particle were generally similar across ethnic groups. The results of this study suggest that Singlish may be acquired as a first language by Singaporean speakers, pre-empting any ethnic differences in the production of the particles that might otherwise have resulted from the speakers’ differing language repertoires.
Exogenous Nitric Oxide and Silicon Applications Alleviate Water Stress in Apricots
Authors: Bakır Asuman , Bolat Ibrahim , Korkmaz Kubra , Hasan Md. , Kaya Ozkan
Published: September 2022

Many plants confront several environmental stresses including heat, water stress, drought, salinity and high-metal concentrations that are crucial in defining plant productivity at different stages of their life cycle. Nitric oxide (NO) and Silicon (Si) are very effective molecules related in most of them and in varied biochemical events that have proven to be protective during cellular injury caused by some stress conditions like water stress. In the current work, we studied the effect of Si and NO alone and NO + Si interactive application on the response of plants exposed to water deficiency and well-watered plants for the Milord apricot variety. Water stress caused a reduce in chlorophyll content, dry and fresh weight, leaf area, stomatal conductivity, leaf relative water content and nutrient elements, while it caused an increase in leaf temperature, leaf proline, leaf malondialdehyde (MDA) content and membrane permeability. Si, NO and Si + NO combination treatments under water stress conditions significantly decreased the adverse effects of water stress on leaf temperature, leaf area, dry and fresh weight, stomata conductivity, relative water content, membrane permeability, L-proline and MDA content. The shoot dry weight, chlorophyll content, stomata conductivity and leaf relative water content in Si + NO treated apricot saplings increased by 59%, 55%, 12% and 8%, respectively. Combined treatment (Si + NO) was detected to be more effective than single applications (Si or NO) on some physiological, biochemical, morphological and nutritional properties of apricot under water stress conditions.
Separation and Extraction of Mixed Grinding Chips of Artificial Joints with Different Densities by Multiple Centrifugal Separations
Authors: Feng Cunao , Zhao Yujie , Zhang Tao , Chen Kai , Li Xiaowei , Zhang Dekun
Published: September 2022

Aseptic loosening caused by the wear and tear of the artificial joint prosthesis after implantation is one of the main causes of artificial joint failure. Therefore, it is important to investigate the wear debris generated due to wear when developing new artificial joint materials. Aseptic loosening is related to the size, number, and morphology of wear debris, and this study proposed the separation and extraction of mixed wear debris with different density ratios of artificial joints by centrifugation to study the characteristics of different artificial joint wear and wear debris extraction rates. The results showed that multiple centrifugations to separate the mixed wear debris were able to reintroduce the wear debris on the wall of the centrifuge tube into the solution and that the wear debris extraction rate was increased. Suspensions with different density ratios of artificially jointed mixed wear debris were effectively separated by this method. The total extraction rate of the three repeated extractions compared to the first extraction rate, the extraction rate of CoCrMo wear debris increased by 6.7%, ultra-high molecular weight polyethylene (UHMWPE) wear debris increased by 15.1–23.44%, ZrO2 wear debris increased by 10.91%, and that of polyether ether ketone (PEEK) wear debris increased by 9.95%. This method for separating and extracting wear debris from artificial joints can realize the separation of mixed wear debris from artificial joints and obtain a high extraction rate and high-quality wear debris images, investigate the wear mechanism of artificial joint implants, and provide valuable information on the wear performance of new artificial joint implants under investigation.
Effect of Flocculants Residue on Rheological Properties of Ultra-Fine Argillaceous Backfilling Slurry
Authors: Li Shuai , Yu Zheng , Hu Boyi , Yu Haoxuan , Wang Xinmin
Published: September 2022

Tailings concentration is indispensable for backfilling. Additionally, the residual flocculants in the concentration process affect the rheological properties of ultra-fine argillaceous backfilling slurry (e.g., viscosity and yield stress), resulting in a great effect on the fluidity and resistance of pipeline transportation. In this study, to explore the effect of flocculants residue on the rheological properties of the slurry, a series of rheological tests (constant shear rate test and variable shear rate test) were performed by changing the type, dosage, stirring time, temperature of flocculants addition and the amount of binder added. The results showed that the addition of flocculants increased the viscosity and yield stress of slurry. At a certain amount of flocculants additive, the flocculant network structure reached the best development state, which had a positive effect on increasing slurry viscosity and yield stress. As the stirring time increased, the scale of damage to the flocculant network structure became larger, which had a negative effect on increasing slurry viscosity and yield stress. Low temperature weakened the adsorption and bridging effect of polymeric chains, resulting in a poorly developed flocculant network structure, which had a negative effect on increasing slurry viscosity and yield stress. Caused by hydration products, the viscosity and yield stress of slurry with binder further increased. This study is significant for an in-depth study of the rheological and pipeline transport characteristics of ultra-fine argillaceous backfilling slurry, optimising the selection of flocculants for ultrafine particles, guiding backfill parameters and improving the reliability of pipeline transport.
Enhanced Design of Gold Catalysts for Bioorthogonal Polyzymes
Authors: Hirschbiegel Cristina-Maria , Fedeli Stefano , Zhang Xianzhi , Huang Rui , Park Jungmi , Xu Yisheng , Rotello Vincent M.
Published: September 2022

Bioorthogonal chemistry introduces nonbiogenic reactions that can be performed in biological systems, allowing for the localized release of therapeutic agents. Bioorthogonal catalysts can amplify uncaging reactions for the in situ generation of therapeutics. Embedding these catalysts into a polymeric nanoscaffold can protect and modulate the catalytic activity, improving the performance of the resulting bioorthogonal “polyzymes”. Catalysts based on nontoxic metals such as gold(I) are particularly attractive for therapeutic applications. Herein, we optimized the structural components of a metal catalyst to develop an efficient gold(I)-based polyzyme. Tailoring the ligand structure of gold phosphine-based complexes, we improved the affinity between the metal complex and polymer scaffold, resulting in enhanced encapsulation efficiency and catalytic rate of the polyzyme. Our findings show the dependence of the overall polyzyme properties on the structural properties of the encapsulated metal complex.
Liquid Moisture Transport in Cotton Woven Fabrics with Different Weft Yarns
Authors: Matusiak Małgorzata , Kamińska Dominika
Published: September 2022

Moisture transport in fabrics influences the thermal comfort of clothing due to drainage of sweat secreted by the human body. The moisture transport through textile materials takes place in two ways: water-vapor transport and liquid moisture transport. Both ways are equally important. In the present work, liquid moisture transport in cotton woven fabrics with different weft yarns was investigated. Measurement was done using the Moisture Management Tester MMT M290. The obtained results confirmed that the linear density of weft yarn significantly influenced the values of all parameters characterizing liquid moisture transport in the investigated fabrics. The best performance in liquid moisture transport was achieved by weft yarn of linear density 30 tex. For this fabric variant, the maximum wetted radius for both surfaces was the biggest: 25 mm for the inner and 26.6 mm for the outer surface of the fabric. This means that the fabric spread the liquid on the biggest area compared to the other variants being investigated to facilitate an evaporation of liquid sweat. The fabric variant with 30 tex weft yarn showed the highest spreading speed: 5.83 mm/s for both sides, and the shortest wetting time: 2.83 s for the inner and 3.00 s for the outer side of the fabric. The higher the linear density of weft yarn, the worse the ability of cotton woven fabrics to ensure liquid moisture transport.
A Mesoscale Study on the Dilation of Actively Confined Concrete under Axial Compression
Authors: Chen Peng , Cui Xiaomeng , Zheng Huijun , Si Shengpu
Published: September 2022

The confinement of concrete enhances its strength and ductility by restraining lateral dilation. The accuracy of a confinement model depends on how well it captures the dilation of concrete. In the current paper, a mesoscale model is established to study the dilation properties of concrete in active confinement, where the heterogeneity of concrete is considered. The stress–strain and lateral–axial strain curves of concrete in active confinement were used to demonstrate the validity of the mesoscale model. Subsequently, the distribution of lateral strain and the influences of the strength grade and confinement ratio on the dilation of concrete were investigated in a simulation. The results show that the distribution of the lateral strain along the radial or longitudinal directions is not uniform on the specimen when compressive failure occurs. The confinement ratio has a more significant influence on the concrete’s transverse dilation than the strength grade. Finally, an expression of the lateral–axial strain relationship of concrete in active confinement is proposed. The proposed formula can reflect the simulation results of the mesoscale model and is in good agreement with the prediction of existing formulas.
Multiscale Simulation of Composite Structures: Damage Assessment, Mechanical Analysis and Prediction
Authors: Georgantzinos Stelios K.
Published: September 2022

Composites can be engineered to exhibit high strength, high stiffness, and high toughness. Composite structures have been used increasingly in various engineering applications. In recent decades, most fundamentals of science have expanded their reach by many orders of magnitude. Currently, one of the primary goals of science and technology seems to be the quest to develop reliable methods for linking the physical phenomena that occur over multiple length scales, particularly from a nano-/micro-scale to a macroscale. The aim of this Special Issue is to assemble high quality papers that advance the field of multiscale simulation of composite structures, through the application of any modern computational and/or analytical methods alone or in conjunction with experimental techniques, for damage assessment or mechanical analysis and prediction.
Dynamic Response and Service Life of Tunnel Bottom Structure Considering Hydro-Mechanical Coupling Effect under the Condition of Bedrock Softening
Authors: Wang Dengke , Luo Jianjun , Li Feilong , Wang Guanqing , Wang Lei , Su Jie , Gao Zhen , Yin Kunyao
Published: September 2022

Due to the long-term coupling effect of a train load and groundwater, the surrounding rock at the tunnel bottom will soften in a certain range and the mechanical parameters of the surrounding rock will decrease, causing the uneven distribution of the confining pressure at the tunnel bottom and affecting the base concrete structure service life. In this research, the method of combining field tests and numerical simulation is adopted, and the vertical displacement, vertical acceleration, and maximum and minimum principal stresses are used as evaluation indicators. The dynamic response law of the base structure with the softened surrounding rock of the heavy-duty train is analyzed, and the Miner linear cumulative damage theory is introduced to obtain the service life of the tunnel bottom structure under different softening conditions. The results show that with the decrease in the softening coefficient and the increase in the softening thickness of the bedrock, the displacement, acceleration, and principal stress response indexes of the structure increase by varying degrees, and the service life of the base structure decreases almost linearly. The maximum vertical displacement, acceleration, and tensile stress are located directly below the track, and the maximum compressive stress is located at the connection between the inverted arch and the side wall. According to the predicted value of the service life, the reliability of the base structure is divided into four levels: safety, warning, danger, and serious danger.
Analysis of the Influence of GMAW Process Parameters on the Properties and Microstructure of S32001 Steel
Authors: Chacón-Fernández Sandra , Portolés García Antonio , Romaní Labanda Gerardo
Published: September 2022

The properties of duplex steels can be seriously affected when welding processes are performed on them. Duplex S32001 is a recent development, so there is very little published information on its behavior after a welding process. For this reason, the present article determines how the main welding parameters of the GMAW process influence its mechanical and microstructural properties. From this study, it was determined that the parameter W/m3 best defines the phase balance in the bead due to the values involved. In the HAZ, this parameter is the t12/8. The welding parameters that are used determine the bead dimensions and geometry. These dimensions induce differences in the distribution of heat in the material. For this reason, the thermal cycles undergone by the material vary and, consequently, in the phase balance obtained. A theoretical study of the chemical composition in the bead, through dilution, and in the HAZ, through diffusion, was carried out. A mathematical model was developed that allows the variation of the composition in the HAZ that induces diffusion to be determined.
Research on the Corrosion Behavior of Q235 Pipeline Steel in an Atmospheric Environment through Experiment
Authors: Cai Shuo , Ji Hongchao , Zhu Fengyun , Pei Weichi , Xiao Wenchao , Tang Xuefeng
Published: September 2022

Low-carbon steel pipelines are frequently used as transport pipelines for various media. As the pipeline transport industry continues to develop in extreme directions, such as high efficiency, long life, and large pipe diameters, the issue of pipeline reliability is becoming increasingly prominent. This study selected Q235 steel, a typical material for low-carbon steel pipelines, as the research object. In accordance with the pipeline service environment and the accelerated corrosion environment test spectrum, cyclic salt spray accelerated corrosion tests that simulated the effects of the marine atmosphere were designed and implemented. Corrosion properties, such as corrosion weight loss, morphology, and product composition of samples with different cycles, were characterized through appearance inspection, scanning electron microscopy analysis, and energy spectrum analysis. The corrosion behavior and mechanism of Q235 low-carbon steel in the enhanced corrosion environment were studied, and the corrosion weight loss kinetics of Q235 steel was verified to conform to the power function law. During the corrosion process, the passivation film on the surface of the low-carbon steel and the dense and stable α-FeOOH layer formed after the passivation film was peeled off played a role in corrosion resistance. The passivation effect, service life, and service limit of Q235 steel were studied and determined, and an evaluation model for quick evaluation of the corrosion life of Q235 low-carbon steel was established. This work provides technical support to improve the life and reliability of low-carbon steel pipelines. It also offers a theoretical basis for further research on the similitude and relevance of cyclic salt spray accelerated corrosion testing.
Properties of Al2O3/Ti/Ni Composite Obtained by Slip Casting with Different Metal Phase Content
Authors: Wachowski Marcin , Zygmuntowicz Justyna , Kosturek Robert , Śnieżek Lucjan , Piotrkiewicz Paulina
Published: September 2022

This work analyzed ceramic-metal composites from the Al2O3/Ti/Ni system produced by the slip casting method. As starting powders, nanometric Al2O3, Ni and Ti of submicron size were used. Three series of composites were obtained and tested with the same solid phase content (50% vol.) and different metallic phase content: 5, 10 and 15% vol. The influence of the metallic phase content on the basic properties of the proposed composites was examined by determining the phase composition of the produced samples using the XRD method, rheological measurements, and microstructural analysis (SEM/EDS). Additionally, before the sintering process, the slip stability was analyzed. A study of the fractography of samples after the sintering process was also carried out. It was shown that the appropriate manufacturing process allowed to obtain NiTi intermetallic phases in the structure of composites.
Study of Heat Transfer and Leakage Characteristics of Brush Seals Based on Local Temperature Non-Equilibrium Model
Authors: Zhang Jiahao , Liu Meihong , Peng Neng
Published: September 2022

In this study, to improve the accuracy of the brush seal heat transfer model, based on the finite volume method (FVM) coupled with the three-dimensional Reynold-averaged Naviers-Stokes equations (RANS) equations of the Local temperature non-equilibrium (LTNE) model, and a mathematical model of the heat transfer and leakage characteristics of the brush seal was established. The distribution of the pressure, flow and temperature fields of the brush seal are analyzed. User-defined function (UDF) programming was performed for the LTNE model. And the LTNE model is then compared with the local temperature equilibrium (LTE) model in terms of the factors influencing the heat transfer and leakage characteristics. The results show that the maximum brush filament temperature increases with an increases in the pressure ratio, interference, and speed for both models; the fluid flow rate increases with an increases in the pressure ratio, interference, and speed; and the leakage rate increases with an increases in the pressure ratio and decreases with an increases in interference and speed. The maximum temperature of the brush filament under the LTNE model was found to be higher than that under the LTE model, but the maximum temperature difference does not exceed 3.1%. Additionally, the fluid flow rate under the LTNE model was higher than that under the LTE model, and the flow rate difference does not exceed 3.4%. And the leakage rate under the LTNE model was lower than that under the LTE model, and the leakage rates differ by no more than 9.0%. Ultimately, numerical analysis of the brush seal under the LTNE model was found to be more effective and consistent with actual working conditions than alternative models.
The Design of Reflected Laser Intensity Testing System and Application of Quality Inspection for Laser Cladding Process
Authors: Zhang Yingtao , Lv Guangming , Li Yaguan , Tang Zirong , Nie Zhenguo
Published: September 2022

Laser cladding is one of the critical technologies for additive manufacturing and rapid repair. Improving cladding performance by materials and process parameters is the leading research direction, but defects and instability of quality in the cladding process are inevitable. Therefore, it is necessary to study which factors are related to quality. In this paper, a new detection method is proposed to measure the radiation intensity of the reflected laser, laser scanning displacement, and temperature of the substrate while cladding. The characteristic values corresponding to the position of the cladding spots are extracted, the cladding quality is preliminarily evaluated and graded, and the correlation between them is verified with the method of machine learning nu-SVM. The results show that the accuracy of the model trained by 300 groups of data to predict the quality grades is 78.74%, which indicates that there is a strong correlation between these process variables and the cladding quality, and this method is feasible for the quality evaluation and control of the cladding process.
Closed-Form Solutions in a Magneto-Electro-Elastic Circular Rod via Generalized Exp-Function Method
Authors: Shakeel Muhammad , Attaullah  , Kbiri Alaoui Mohammed , Zidan Ahmed M. , Shah Nehad Ali , Weera Wajaree
Published: September 2022

In this study, the dispersal caused by the transverse Poisson’s effect in a magneto-electro-elastic (MEE) circular rod is taken into consideration using the nonlinear longitudinal wave equation (LWE), a mathematical physics problem. Using the generalized exp-function method, we investigate the families of solitary wave solutions of one-dimensional nonlinear LWE. Using the computer program Wolfram Mathematica 10, these new exact and solitary wave solutions of the LWE are derived as trigonometric function, periodic solitary wave, rational function, hyperbolic function, bright and dark solitons solutions, sinh, cosh, and sech2 function solutions of the LWE. These solutions represent the electrostatic potential and pressure for LWE as well as the graphical representation of electrostatic potential and pressure.
Application of Simplified Homogeneous Balance Method to Multiple Solutions for (2 + l)-Dimensional Burgers’ Equations
Authors: Li Lingxiao , Zhang Jinliang , Wang Mingliang
Published: September 2022

In this paper, three forms of (2 + l)-dimensional Burgers’ equations are investigated. More general multiple solutions of these Burgers’ equations are obtained by dependent variable transformation derived using the simplified homogeneous balance method.
Entropy Analysis for Hydromagnetic Darcy–Forchheimer Flow Subject to Soret and Dufour Effects
Authors: Khan Sohail A. , Hayat Tasawar
Published: September 2022

Here, our main aim is to examine the impacts of Dufour and Soret in a radiative Darcy–Forchheimer flow. Ohmic heating and the dissipative features are outlined. The characteristics of the thermo-diffusion and diffusion-thermo effects are addressed. A binary chemical reaction is deliberated. To examine the thermodynamical system performance, we discuss entropy generation. A non-linear differential system is computed by the finite difference technique. Variations in the velocity, concentration, thermal field and entropy rate for the emerging parameters are scrutinized. A decay in velocity is observed for the Forchheimer number. Higher estimation of the magnetic number has the opposite influence for the velocity and temperature. The velocity, concentration and thermal field have a similar effect on the suction variable. The temperature against the Dufour number is augmented. A decay in the concentration is found against the Soret number. A similar trend holds for the entropy rate through the radiation and diffusion variables. An augmentation in the entropy rate is observed for the diffusion variable.
3D Printed and Bioprinted Membranes and Scaffolds for the Periodontal Tissue Regeneration: A Narrative Review
Authors: Sufaru Irina-Georgeta , Macovei Georgiana , Stoleriu Simona , Martu Maria-Alexandra , Luchian Ionut , Kappenberg-Nitescu Diana-Cristala , Solomon Sorina Mihaela
Published: September 2022

Numerous technologies and materials were developed with the aim of repairing and reconstructing the tissue loss in patients with periodontitis. Periodontal guided bone regeneration (GBR) and guided tissue regeneration (GTR) involves the use of a membrane which prevents epithelial cell migration, and helps to maintain the space, creating a protected area in which tissue regeneration is favored. Over the time, manufacturing procedures of such barrier membranes followed important improvements. Three-dimensional (3D) printing technology has led to major innovations in periodontal regeneration methods, using technologies such as inkjet printing, light-assisted 3D printing or micro-extrusion. Besides the 3D printing of monophasic and multi-phasic scaffolds, bioprinting and tissue engineering have emerged as innovative technologies which can change the way we see GTR and GBR.
Critical Assessment of Membrane Technology Integration in a Coal-Fired Power Plant
Authors: Alabid Maytham , Cormos Calin-Cristian , Dinca Cristian
Published: September 2022

Despite the many technologies for CO2 capture (e.g., chemical or physical absorption or adsorption), researchers are looking to develop other technologies that can reduce CAPEX and OPEX costs as well as the energy requirements associated with their integration into thermal power plants. The aim of this paper was to analyze the technical and economic integration of spiral wound membranes in a coal-fired power plant with an installed capacity of 330 MW (the case of the Rovinari power plant—in Romania). The study modeled energy processes using CHEMCAD version 8.1 software and polymer membranes developed in the CO2 Hybrid research project. Thus, different configurations such as a single membrane step with and without the use of a vacuum pump and two membrane steps placed in series were analyzed. In all cases, a compressor placed before the membrane system was considered. The use of two serialized stages allows for both high efficiency (minimum 90%) and CO2 purity of a minimum of 95%. However, the overall plant efficiency decreased from 45.78 to 23.96% and the LCOE increased from 75.6 to 170 €/kWh. The energy consumption required to capture 1 kg of CO2 is 2.46 MJel and 4.52 MJth.
Martensitic Transition and Superelasticity of Ordered Heat Treatment Ni-Mn-Ga-Fe Microwires
Authors: Liu Yanfen , Lang Zirui , Shen Hongxian , Liu Jingshun , Sun Jianfei
Published: September 2022

The preparation of Ni-Mn-Ga and Ni-Mn-Ga-Fe master alloy ingots and microwires was completed by high vacuum electric furnace melt melting furnace and melt drawing liquid forming equipment, and the lattice dislocations and defects formed inside the microwires during the preparation process were corrected by stepwise ordered heat treatment. The micro-structure and phase structure were characterized using a SEM field emission scanning electron microscopy and an XRD diffractometer combined with an EDS energy spectrum analyzer; the martensitic phase transformation process of the microwires was analyzed using a DSC differential scanning calorimeter; and the superelasticity of the microwires was tested by a Q800 dynamic mechanical analyzer. The results indicate that Fe doping can refine the grain, transform the phase structure from parent phase to single 7M martensite, reduce the number of martensitic variants, and increase the mobility of the twin grain boundary interface. The MT phase transition temperature (MS) is substantially increased in the martensite transition (MT) process by the increase of the number of free electrons in its lattice. During the superelasticity (SE) test, both microwires displayed superior recover-ability of SE curves, and the Fe doping curves showed similar characteristics of “linear superelasticity”, showing higher critical stress values and complete SE in the experiment. The critical stress satisfies the Clausius-Clapeyron equation and exhibits higher temperature sensitivity than Ni-Mn-Ga microwires.
An Overview on Atomistic Mechanisms of Heterogeneous Nucleation
Authors: Fan Zhongyun , Men Hua
Published: September 2022

Our current understanding of heterogeneous nucleation has been dominated by the classical nucleation theory (CNT) with little progress of significance being made in past 100 years. In recent years under the financial support from EPSRC for the LiME Research Hub, we have made substantial progress on understanding heterogeneous nucleation at atomic level using a combination of molecular dynamics simulations and advanced high-resolution electron microscopy. We found that heterogeneous nucleation proceeds through a three-layer nucleation mechanism to produce a 2D nucleus. The atomistic mechanisms responsible for accommodating lattice misfit are dependent on misfit (f): (1) for systems with small negative misfit (−12.5% < f < 0), misfit is accommodated by dislocation mechanism; (2) for systems with small positive misfit (0 < f < 12.5%), misfit is accommodated by vacancy mechanism; and (3) for systems with large misfit (|f| > 12.5%), misfit is accommodated in two steps: formation of coincidence site lattice during prenucleation to accommodate the major misfit (fcsL) and the residual misfit (fr) is accommodated during heterogeneous nucleation by the dislocation mechanism if the residual misfit is less than 0 or by the vacancy mechanism if the residual misfit is larger than 0. Further analysis suggests that heterogeneous nucleation is spontaneous thus barrierless and deterministic rather than stochastic.
Non-Invasive Differential Diagnosis of Cervical Neoplastic Lesions by the Lipid Profile Analysis of Cervical Scrapings
Authors: Tokareva Alisa , Chagovets Vitaliy , Attoeva Djamilja , Starodubtseva Natalia , Nazarova Niso , Gusakov Kirill , Kukaev Eugenii , Frankevich Vladimir , Sukhikh Gennady
Published: September 2022

Cervical cancer is one of the most common cancers in women with pronounced stages of precancerous lesions. Accurate differential diagnosis of such lesions is one of the primary challenges of medical specialists, which is vital to improving patient survival. The aim of this study was to develop and test an algorithm for the differential diagnosis of cervical lesions based on lipid levels in scrapings from the cervical epithelium and cervicovaginal canal. The lipid composition of the samples was analyzed by high-performance chromato-mass spectrometry. Lipid markers were selected using the Mann–Whitney test with a cutoff value of 0.05 and by projections to latent structures discriminant analysis, where a projection threshold of one was chosen. The final selection of variables for binomial logistic regressions was carried out using the Akaike information criterion. As a result, a final neoplasia classification method, based on 20 logistic regression sub-models, has an accuracy of 79% for discrimination NILM/cervicitis/LSIL/HSIL/cancer. The model has a sensitivity of 83% and a specificity of 88% for discrimination of several lesions (HSIL and cancer). This allows us to discuss the prospective viability of further validation of the developed non-invasive method of differential diagnosis.
Fabrication of Concave Microwells and Their Applications in Micro-Tissue Engineering: A Review
Authors: Guo Weijin , Chen Zejingqiu , Feng Zitao , Li Haonan , Zhang Muyang , Zhang Huiru , Cui Xin
Published: September 2022

At present, there is an increasing need to mimic the in vivo micro-environment in the culture of cells and tissues in micro-tissue engineering. Concave microwells are becoming increasingly popular since they can provide a micro-environment that is closer to the in vivo environment compared to traditional microwells, which can facilitate the culture of cells and tissues. Here, we will summarize the fabrication methods of concave microwells, as well as their applications in micro-tissue engineering. The fabrication methods of concave microwells include traditional methods, such as lithography and etching, thermal reflow of photoresist, laser ablation, precision-computerized numerical control (CNC) milling, and emerging technologies, such as surface tension methods, the deformation of soft membranes, 3D printing, the molding of microbeads, air bubbles, and frozen droplets. The fabrication of concave microwells is transferring from professional microfabrication labs to common biochemical labs to facilitate their applications and provide convenience for users. Concave microwells have mostly been used in organ-on-a-chip models, including the formation and culture of 3D cell aggregates (spheroids, organoids, and embryoids). Researchers have also used microwells to study the influence of substrate topology on cellular behaviors. We will briefly review their applications in different aspects of micro-tissue engineering and discuss the further applications of concave microwells. We believe that building multiorgan-on-a-chip by 3D cell aggregates of different cell lines will be a popular application of concave microwells, while integrating physiologically relevant molecular analyses with the 3D culture platform will be another popular application in the near future. Furthermore, 3D cell aggregates from these biosystems will find more applications in drug screening and xenogeneic implantation.
Chlorellestadite (Synth): Formation, Structure, and Carbonate Substitution during Synthesis of Belite Clinker from Wastes in the Presence of CaCl2 and CO2
Authors: Garbev Krassimir , Ullrich Angela , Beuchle Günter , Bergfeldt Britta , Stemmermann Peter
Published: September 2022

The synthesis of low-temperature belite (C2S) clinker from wastes of autoclaved aerated concrete and limestone was studied in the presence of CaCl2 as a mineralizing agent. Synthetic chlorellestadite (SCE; Ca10(SiO4)3(SO4)3Cl2) forms in experiments at temperatures between 700 and 1200 °C. Samples were investigated by X-ray diffraction and Raman spectroscopy. In general, the amount of SCE depends mainly on the sulfate content and to a lesser extent on the synthesis temperature. At lower temperatures of formation, a non-stoichiometric SCE seems to crystallize in a monoclinic symmetry similar to hydroxylellestadite. Rietveld refinements revealed the presence of chlorine and calcium vacancies. Raman spectroscopy proved the partial substitution of sulfate by CO32− groups in ellestadites formed at 800 °C and 900 °C in air. Incorporation of CO3 results in a shorter unit cell parameters and smaller cell volume similar to CO3−apatite. At low temperatures, SCE coexists with spurrite intermixed on a very fine nm scale. At temperatures above 900 °C in air, ellestadite is carbonate-free and above 1000 °C chlorine loss starts in all samples.