bluuebunny/crossref_metadata_2025 huggingface dataset:
Biting midges (Diptera: Ceratopogonidae) as putative vectors of zoonotic Onchocerca lupi (Nematoda: Onchocercidae) in northern Arizona and New Mexico, southwestern United States
Authors: Roe Chandler C. , Holiday Olivia , Upshaw-Bia Kelly , Benally Gaven , Williamson Charles H. D. , Urbanz Jennifer , Verocai Guilherme G. , Ridenour Chase L. , Nottingham Roxanne , Ford Morgan A. , Lake Derek P. , Kennedy Theodore A. , Hepp Crystal M. , Sahl Jason W.
Published: May 2023

Onchocerca lupi (Rodonaja, 1967) is an understudied, vector-borne, filarioid nematode that causes ocular onchocercosis in dogs, cats, coyotes, wolves, and is also capable of infecting humans. Onchocercosis in dogs has been reported with increasing incidence worldwide. However, despite the growing number of reports describing canine O. lupi cases as well as zoonotic infections globally, the disease prevalence in endemic areas and vector species of this parasite remains largely unknown. Here, our study aimed to identify the occurrence of O. lupi infected dogs in northern Arizona, New Mexico, and Utah, United States and identify the vector of this nematode. A total of 532 skin samples from randomly selected companion animals with known geographic locations within the Navajo Reservation were collected and molecularly surveyed by PCR for the presence of O. lupi DNA (September 2019–June 2022) using previously published nematode primers (COI) and DNA sequencing. O. lupi DNA was detected in 50 (9.4%) sampled animals throughout the reservation. Using positive animal samples to target geographic locations, pointed hematophagous insect trapping was performed to identify potential O. lupi vectors. Out of 1,922 insects screened, 38 individual insects and 19 insect pools tested positive for the presence of O. lupi, all of which belong to the Diptera family. This increased surveillance of definitive host and biological vector/intermediate host is the first large scale prevalence study of O. lupi in companion animals in an endemic area of the United States, and identified an overall prevalence of 9.4% in companion animals as well as multiple likely biological vector and putative vector species in the southwestern United States. Furthermore, the identification of these putative vectors in close proximity to human populations coupled with multiple, local zoonotic cases highlight the One Health importance of O. lupi.
Additive Manufacturing of MnAl(C)-Magnets
Authors: Pacheco Victor , Skårman Björn , Olsson Fredrik , Karlsson Dennis , Vidarsson Hilmar , Sahlberg Martin
Published: May 2023

Permanent magnets are becoming more and more relevant for modern society. As the most widely used permanent magnets contain rare-earth elements, the increased dependence on these strategic elements is worrisome, and the pursuit for rare-earth free alternatives has become a strategic goal in many countries. The metastable and ferromagnetic τ-phase that forms in the MnAl(C) system is one of the most promising alternatives, and since its discovery, major efforts have been made to improve its performance and realize its full potential. One major factor that has prevented a widespread commercialization of MnAl(C) permanent magnets is their relatively low coercivity. Here, we demonstrate that additive manufacturing, using laser powder bed fusion, can be used to produce MnAl in its high-temperature polymorph (ε, hcp), which can be subsequently transformed, through post-heat treatments to the ferromagnetic τ-phase. Although we successfully obtained a preferential orientation of the ε-phase with <001> parallel to the build direction, this did not translate into a strong preferential orientation in the τ-phase, thus indicating that the phase transformation occurs by the migration of incoherent interfaces. The MnAl(C) samples are characterized by a density of ≈4.4 g/cm3, a saturation magnetization of 39.3 Am2/kg, a coercivity of 168 kA/m, and a remanence of 17.5 Am2/kg.
Comparative Analysis of AI-Based Facial Identification and Expression Recognition Using Upper and Lower Facial Regions
Authors: Kim Seunghyun , An Byeong Seon , Lee Eui Chul
Published: May 2023

The COVID-19 pandemic has significantly impacted society, having led to a lack of social skills in children who became used to interacting with others while wearing masks. To analyze this issue, we investigated the effects of masks on face identification and facial expression recognition, using deep learning models for these operations. The results showed that when using the upper or lower facial regions for face identification, the upper facial region allowed for an accuracy of 81.36%, and the lower facial region allowed for an accuracy of 55.52%. Regarding facial expression recognition, the upper facial region allowed for an accuracy of 39% compared to 49% for the lower facial region. Furthermore, our analysis was conducted for a number of facial expressions, and specific emotions such as happiness and contempt were difficult to distinguish using only the upper facial region. Because this study used a model trained on data generated from human labeling, it is assumed that the effects on humans would be similar. Therefore, this study is significant because it provides engineering evidence of a decline in facial expression recognition; however, wearing masks does not cause difficulties in identification.
Effect of Desert Sand on the Section Bonding Properties of Polyethylene Fiber−Engineered Cementitious Composites
Authors: Niu Yanfeng , Han Fengxia , Liu Qing , Yang Xu
Published: May 2023

Xinjiang is in northwest China and has abundant desert sand. Replacing natural sand with sand from deserts is an urgent need and could be used in making polyethylene fiber−engineered cementitious composite (PE−ECC). The interfacial bonding properties of desert sand PE−ECC (DSPE−ECC) were made using the optimal mix proportion (30% desert sand content, 2% fiber volume) and the laboratory’s previous research results. Normal sand PE−ECC (NSPE−ECC) and DSPE-ECC at different test ages (3, 7, 14, and 28 days) were subjected to uniaxial tensile tests, and a method for determining bonding properties is proposed. Scanning electron microscopy and X-ray diffraction were used to analyze the development of PE-ECC fiber and matrix and the formation of hydration products. The results indicated that the cracking loads of the DSPE-ECC at 3, 7, 14, and 28 days increased by 16.72%, 28%, 23.23%, and 10.05%, respectively. Desert sand had low water content and high water absorption, which slowed down the rate of C2S, C3S combining with water molecules to form C−S−H, and had a great influence on the bonding properties of ECC at 3 days. However, the bonding properties of DSPE−ECC were only slightly less than those of NSPE−ECC at 28 days, and the bonding properties had gradually stabilized. Therefore, the addition of desert sand enhanced the fiber/matrix’s bonding properties, and the bonding properties stablized with the increase in curing ages.
Psychobiotics as an Intervention in the Treatment of Irritable Bowel Syndrome: A Systematic Review
Authors: Gwioździk Weronika , Helisz Paulina , Grajek Mateusz , Krupa-Kotara Karolina
Published: May 2023

Today’s perceived excess of work versus rest and excessive stressful situations are one of the potential factors for an increasing number of gastrointestinal disorders. Although the prevalence of irritable bowel syndrome (IBS) varies widely, it is assumed that up to 15% of the general population may be affected. Symptoms resulting from IBS take the form of pain, including difficulties with bowel movements, bloating, and a feeling of overflowing in the intestines. These symptoms have a negative impact on patients’ quality of life, so any possible intervention supported by Evidence-Based Medicine should effectively contribute to their improvement. In the following review, the authors compile the current knowledge based on the findings of researchers from around the world. The topic of probiotic therapy is increasingly discussed in the field of lower gastrointestinal disorders, and thus the authors discover links between probiotic strains and the improvement of health quality in patients with IBS, taking into consideration the ways it affects patients’ mental health. As a result, a systematic review was prepared based on 60 items of recent literature, of which more than 80% were published in the last 5 years.
Spatiotemporal Precipitation Trends and Associated Large-Scale Teleconnections in Northern Pakistan
Authors: Rebi Ansa , Hussain Azfar , Hussain Ishtiaq , Cao Jianhua , Ullah Waheed , Abbas Haider , Ullah Safi , Zhou Jinxing
Published: May 2023

The effects of climate change are unparalleled in magnitude, ranging from changing weather patterns that endanger food production to increasing sea levels that increase the likelihood of catastrophic flooding. Therefore, determining the extent of such variations on regional and local scales is imperative. We used monthly precipitation data from 25 meteorological stations in northern Pakistan (NP) to document the observed changes in seasonal and annual precipitation. The station density in the NP is small and unevenly distributed; therefore, ERA-5 reanalysis data were used to supplement the observed dataset to assess the spatial trends in NP. The non-parametric Mann–Kendall (MK), Sen’s Slope estimator (SSE), and Sequential Mann–Kendall (SQMK) tests were performed to assess the trends. In addition, the wavelet analysis technique was used to determine the association of precipitation with various oceanic indices from 1960 to 2016. Results indicate that maximum precipitation was shown in the annual and summer seasons. In NP, annual, winter, spring, and summer precipitation declined, while an increase in autumn was observed at a rate of 0.43 mm/decade between 1989 and 2016. The spatial trends for observed and ERA-5 reanalysis datasets were almost similar in winter, spring, and autumn; however, some disagreement was observed in both datasets in the summer and annual precipitation trends in NP during 1960–2016. Between 1989 and 2016, summer and annual precipitation increased significantly in Region III. However, seasonal and annual precipitation decreased in NP between 1960 and 2016. Moreover, there were no prominent trends in annual precipitation until the mid-1980s, but an apparent increase from 1985 onwards. Annual precipitation increased in all elevations except at the 500–1000 m zone. The ENSO (El Niño–Southern Oscillation) shared notable interannual coherences among all indices above 16–64 months. Inter-decadal coherence with the ENSO, AO (Arctic Oscillation), and PDO (Pacific Decadal Oscillation) in NP for 128 months and above. Generally, AO, AMO (Atlantic Multidecadal Oscillation), and NAO (North Atlantic Oscillation) exhibited less coherence with precipitation in NP. The regression of seasonal and annual precipitation revealed that winter and spring precipitation levels had higher linear regression with the AO and ENSO, respectively, while both the AO and ENSO also dominated at the annual scale. Similarly, the IOD and PDO indices had a higher influence in summer precipitation. The findings may help water resource managers and climate researchers develop a contingency plan for better water resource management policies in the face of changing climate change in Pakistan, particularly in NP.
Recent Developments in Protein Lactylation in PTSD and CVD: Novel Strategies and Targets
Authors: Kozlakidis Zisis , Shi Patricia , Abarbanel Ganna , Klein Carolina , Sfera Adonis
Published: May 2023

In 1938, Corneille Heymans received the Nobel Prize in physiology for discovering that oxygen sensing in the aortic arch and carotid sinus was mediated by the nervous system. The genetics of this process remained unclear until 1991 when Gregg Semenza while studying erythropoietin, came upon hypoxia-inducible factor 1, for which he obtained the Nobel Prize in 2019. The same year, Yingming Zhao found protein lactylation, a posttranslational modification that can alter the function of hypoxia-inducible factor 1, the master regulator of cellular senescence, a pathology implicated in both post-traumatic stress disorder (PTSD) and cardiovascular disease (CVD). The genetic correlation between PTSD and CVD has been demonstrated by many studies, of which the most recent one utilizes large-scale genetics to estimate the risk factors for these conditions. This study focuses on the role of hypertension and dysfunctional interleukin 7 in PTSD and CVD, the former caused by stress-induced sympathetic arousal and elevated angiotensin II, while the latter links stress to premature endothelial cell senescence and early vascular aging. This review summarizes the recent developments and highlights several novel PTSD and CVD pharmacological targets. They include lactylation of histone and non-histone proteins, along with the related biomolecular actors such as hypoxia-inducible factor 1α, erythropoietin, acid-sensing ion channels, basigin, and Interleukin 7, as well as strategies to delay premature cellular senescence by telomere lengthening and resetting the epigenetic clock.
DNA Methyltransferase 1 Targeting Using Guadecitabine Inhibits Prostate Cancer Growth by an Apoptosis-Independent Pathway
Authors: Karan Dev , Singh Manohar , Dubey Seema , Van Veldhuizen Peter J. , Saunthararajah Yogen
Published: May 2023

Epigenetic alterations such as DNA methylation and histone modifications are implicated in repressing several tumor suppressor genes in prostate cancer progression. In this study, we determined the anti-prostate cancer effect of a small molecule drug guadecitabine (gDEC) that inhibits/depletes the DNA methylation writer DNA methyltransferase 1 (DNMT1). gDEC inhibited prostate cancer cell growth and proliferation in vitro without activating the apoptotic cascade. Molecular studies confirmed DNMT1 depletion and modulated epithelial-mesenchymal transition markers E-cadherin and β-catenin in several prostate cancer cell lines (LNCaP, 22Rv1, and MDA PCa 2b). gDEC treatment also significantly inhibited prostate tumor growth in vivo in mice (22Rv1, MDA PCa 2b, and PC-3 xenografts) without any observed toxicities. gDEC did not impact the expression of androgen receptor (AR) or AR-variant 7 (AR-V7) nor sensitize the prostate cancer cells to the anti-androgen enzalutamide in vitro. In further investigating the mechanism of cytoreduction by gDEC, a PCR array analyses of 84 chromatin modifying enzymes demonstrated upregulation of several lysine-specific methyltransferases (KMTs: KMT2A, KMT2C, KMT2E, KMT2H, KMT5A), confirmed by additional expression analyses in vitro and of harvested xenografts. Moreover, gDEC treatment increased global histone 3 lysine 4 mono-and di-methylation (H3K4me1 and H3K4me2). In sum, gDEC, in addition to directly depleting the corepressor DNMT1, upregulated KMT activating epigenetic enzymes, activating terminal epithelial program activation, and prostate cancer cell cycling exits independent of apoptosis.
Reproducibility in PD-L1 Immunohistochemistry Quantification through the Tumor Proportion Score and the Combined Positive Score: Could Dual Immunostaining Help Pathologists?
Authors: Mercier Anaïs , Conan-Charlet Virginie , Quintin-Roué Isabelle , Doucet Laurent , Marcorelles Pascale , Uguen Arnaud
Published: May 2023

We studied the pathologists’ agreements in quantifying PD-L1 expression through the tumor proportion score (TPS) and the combined positive score (CPS) using single PD-L1 immunohistochemistry (S-IHC) and double immunohistochemistry (D-IHC) combining PD-L1 staining and tumor cell markers. S-IHC and D-IHC were applied to 15 cancer samples to generate 60 digital IHC slides (30 whole slides images and 30 regions of interest of 1 mm2) for PD-L1 expression quantification using both TPS and CPS, twice by four pathologists. Agreements were estimated calculating intraclass correlation coefficients (ICC). Both S-IHC and D-IHC slides analyses resulted in excellent (for TPS, ICC > 0.9) to good (for CPS, ICC > 0.75) inter- and intra-pathologist agreements with slightly higher ICC with D-IHC than with S-IHC. S-IHC resulted in higher TPS and CPS than D-IHC (+5.6 and +6.1 mean differences, respectively). High reproducibility in the quantification of PD-L1 expression is attainable using S-IHC and D-IHC.
The Devastating Effects of Sleep Deprivation on Memory: Lessons from Rodent Models
Authors: Chen Pinqiu , Ban Weikang , Wang Wenyan , You Yuyang , Yang Zhihong
Published: May 2023

In this narrative review article, we discuss the role of sleep deprivation (SD) in memory processing in rodent models. Numerous studies have examined the effects of SD on memory, with the majority showing that sleep disorders negatively affect memory. Currently, a consensus has not been established on which damage mechanism is the most appropriate. This critical issue in the neuroscience of sleep remains largely unknown. This review article aims to elucidate the mechanisms that underlie the damaging effects of SD on memory. It also proposes a scientific solution that might explain some findings. We have chosen to summarize literature that is both representative and comprehensive, as well as innovative in its approach. We examined the effects of SD on memory, including synaptic plasticity, neuritis, oxidative stress, and neurotransmitters. Results provide valuable insights into the mechanisms by which SD impairs memory function.
Plasma-Deposited Organosilicon Hydrophobic Coatings on Cellulosic Materials for Wet Packaging Applications
Authors: Profili Jacopo , Babaei Sara , Al Rashidi Mariam , Dorris Annie , Asadollahi Siavash , Sarkissian Andranik , Stafford Luc
Published: May 2023

Non-toxic organosilicon coatings with hydrophobic properties were deposited on Kraft paper films using atmospheric-pressure dielectric barrier discharge. In order to assess the applicability of the plasma-deposited coating in the food packaging industry, its stability in different liquids (i.e., food simulants) was studied. Chemical analyses reveal that the food simulants, namely, de-ionized water, acetic acid, ethanol, and heptane, do not significantly alter the chemical composition or the hydrophobicity of the coatings. Based on inductively coupled plasma-optical emission spectroscopy analyses, the amount of Si released from the plasma-deposited coating is less than that typically detected in tap water. Overall, the results obtained suggest that hydrophobic plasma-deposited organosilicon coatings have great potential for use in food and wet packaging applications, especially considering their environmentally friendly character.
Defining Evolutionary Conservation Units in the Macedonian Crested Newt, Triturus macedonicus (Amphibia; Salamandridae), in a Biodiversity Hotspot
Authors: Danelis Taxiarchis , Theodoropoulos Anagnostis , Toli Elisavet-Aspasia , Bounas Anastasios , Korakis Athanasios , Sotiropoulos Konstantinos
Published: May 2023

In this study, we used genetic approaches to assess the conservation status of a protected amphibian species, the Macedonian crested newt, Triturus macedonicus, in Northern Pindos National Park (Epirus, Greece). Mitochondrial DNA sequences and multilocus genotypes of individuals from 38 breeding sites were used to infer their phylogenetic position and to detect and measure genetic variation patterns, population genetic structure, and levels of gene flow. The examined individuals fell within two major clades of the Macedonian crested newt phylogeny, being geographically separated by the Aoos River valley and Vikos Gorge. Both groups constitute separate gene pools, bearing private haplotypes and alleles, and the groups were found to be highly differentiated in both their mitochondrial and microsatellite markers. Thus, they meet all of the criteria needed to be characterized as evolutionary significant units (ESUs) that deserve a separate conservation status. Within each ESU, the particularly high inter-population differentiation and low migration rates imply a lack of connectivity between breeding sites and local isolation in favorable habitats. Our results can inform future programs and actions towards the conservation and management of the Macedonian crested newt in the area.
Reviewing Evolution of Learning Functions and Semantic Information Measures for Understanding Deep Learning
Authors: Lu Chenguang
Published: May 2023

A new trend in deep learning, represented by Mutual Information Neural Estimation (MINE) and Information Noise Contrast Estimation (InfoNCE), is emerging. In this trend, similarity functions and Estimated Mutual Information (EMI) are used as learning and objective functions. Coincidentally, EMI is essentially the same as Semantic Mutual Information (SeMI) proposed by the author 30 years ago. This paper first reviews the evolutionary histories of semantic information measures and learning functions. Then, it briefly introduces the author’s semantic information G theory with the rate-fidelity function R(G) (G denotes SeMI, and R(G) extends R(D)) and its applications to multi-label learning, the maximum Mutual Information (MI) classification, and mixture models. Then it discusses how we should understand the relationship between SeMI and Shannon’s MI, two generalized entropies (fuzzy entropy and coverage entropy), Autoencoders, Gibbs distributions, and partition functions from the perspective of the R(G) function or the G theory. An important conclusion is that mixture models and Restricted Boltzmann Machines converge because SeMI is maximized, and Shannon’s MI is minimized, making information efficiency G/R close to 1. A potential opportunity is to simplify deep learning by using Gaussian channel mixture models for pre-training deep neural networks’ latent layers without considering gradients. It also discusses how the SeMI measure is used as the reward function (reflecting purposiveness) for reinforcement learning. The G theory helps interpret deep learning but is far from enough. Combining semantic information theory and deep learning will accelerate their development.
The Complete Mitochondrial Genome and Phylogenetic Analyses of To Chicken in Vietnam
Authors: Pham Lan Doan , Giang Thi Thanh Nhan , Nguyen Van Ba , Pham Thi Phuong Mai , Tran Thi Thu Thuy , Nguyen Thi Quynh Chau , Van Nguyen Khanh , Do Duy Ngoc
Published: May 2023

Indigenous chicken breeds have both cultural significance and economic value since they possess unique genetic characteristics that enable them to adapt to the local environment and contribute to biodiversity, food security, and sustainable agriculture in Vietnam. To (Tò in Vietnamese) chicken, a Vietnamese indigenous chicken breed, is popularly raised in Thai Binh province; however, little known is about the genetic diversity of this breed. In this study, we sequenced the complete mitochondrial genome of To chicken for a better understanding of the diversity and origin of the breed. The results of sequencing showed that the mitochondrial genome of To chicken spans a total length of 16,784 base pairs and comprises one non-coding control region (known as the displacement-loop (D-loop) region), two ribosomal RNA genes, 13 protein-coding genes, and 22 transfer RNA genes. The phylogenetic tree analyses and estimated genetic distances based on 31 complete mitochondrial genome sequences indicated that To chicken has a close genetic distance with the Laotian native chicken breed, Lv’erwu breed in China, and Nicobari black and Kadaknath breeds in India. The result of the current study might be important for conservation, breeding, and further genetic studies of To chicken.
Genome-Wide Association Mapping for Yield and Yield-Related Traits in Rice (Oryza Sativa L.) Using SNPs Markers
Authors: Ashfaq Muhammad , Rasheed Abdul , Zhu Renshan , Ali Muhammad , Javed Muhammad Arshad , Anwar Alia , Tabassum Javaria , Shaheen Shabnum , Wu Xianting
Published: May 2023

Rice (Oryza sativa L.) is a staple food for more than 50% of the world’s population. Rice cultivar improvement is critical in order to feed the world’s growing population. Improving yield is one of the main aims of rice breeders. However, yield is a complex quantitative trait controlled by many genes. The presence of genetic diversity is the key factor to improve the yield hence, the presence of diversity in any germplasm is important for yield improvement. In the current study, the rice germplasm was collected from Pakistan and the United States of America and a panel of 100 diverse genotypes was utilized to identify important yield and yield-related traits. For this, a genome-wide association study (GWAS) was performed to identify the genetic loci related to yield. The GWAS on the diverse germplasm will lead to the identification of new genes which can be utilized in the breeding program for improvement of yield. For this reason, firstly, the germplasm was phenotypically evaluated in two growing seasons for yield and yield-related traits. The analysis of variance results showed significant differences among traits which showed the presence of diversity in the current germplasm. Secondly, the germplasm was also genotypically evaluated using 10K SNP. Genetic structure analysis showed the presence of four groups which showed that enough genetic diversity was present in the rice germplasm to be used for association mapping analysis. The results of GWAS identified 201 significant marker trait associations (MTAs. 16 MTAs were identified for plant height, 49 for days to flowering, three for days to maturity, four for tillers per plant, four for panicle length, eight for grains per panicle, 20 unfilled grains per panicle, 81 for seed setting %, four for thousand-grain weight, five for yield per plot and seven for yield per hectare. Apart from this, some pleiotropic loci were also identified. The results showed that panicle length (PL) and thousand-grain weight (TGW) were controlled by a pleiotropic locus OsGRb23906 on chromosome 1 at 10,116,371 cM. The loci OsGRb25803 and OsGRb15974 on chromosomes 4 and 8 at the position of 14,321,111 cM and 6,205,816 cM respectively, showed pleiotropic effects for seed setting % (SS) and unfilled grain per panicle (UG/P). A locus OsGRb09180 on chromosome 4 at 19,850,601 cM was significantly linked with SS and yield/ha. Furthermore, gene annotation was performed, and results indicated that the 190 candidate genes or QTLs that closely linked with studied traits. These candidate genes and novel significant markers could be useful in marker-assisted gene selection and QTL pyramiding to improve rice yield and the selection of potential parents, recombinants and MTAs which could be used in rice breeding programs to develop high-yielding rice varieties for sustainable food security.
Optimising Kelp Cultivation to Scale up Habitat Restoration Efforts: Effect of Light Intensity on “Green Gravel” Production
Authors: Chemello Silvia , Pinto Isabel Sousa , Pereira Tania R.
Published: May 2023

Kelp forests are disappearing worldwide due to climate change and human stressors, highlighting the need for active interventions. A new restoration approach, “green gravel”, has been shown to be a potentially effective tool to restore endangered kelp forests. However, green gravel is still a novel technique that needs better experimentation and optimisation at all process stages. Contamination by other algal species is one of the critical factors in early-stage green gravel production because their overgrowth can lead to the loss of the seeded material. In this study, we assessed the effect of light intensity on kelp growth and on the coverage of contaminating algae on green gravel. Our results show that under high lights, kelps displayed faster growth (recruits on average more than three times the size and covering a six-times-larger area in high light intensity than in low light), but there was also a higher percentage of contaminating algae. In contrast, the green gravel cultivated under low lights showed almost no signs of algal contamination, but the area occupied by kelps and the length of the lamina were dramatically lower. Due to the cultivation conditions, opportunistic species can grow fast. This advantage is expected to disappear once the green gravel is deployed. To obtain cleaner cultures and to avoid the risk of losing the cultivated material, we would advise starting rearing under lower light intensity to reduce the risk of contamination but ensure kelp growth and then increasing the light intensity to boost it. Clear and appropriate protocols are absolutely necessary to minimise production costs and times and for the scaling-up of future attempts at marine forest restoration.
Has the COVID-19 Pandemic Affected Cyberbullying and Cybervictimization Prevalence among Children and Adolescents? A Systematic Review
Authors: Sorrentino Anna , Sulla Francesco , Santamato Margherita , di Furia Marco , Toto Giusi , Monacis Lucia
Published: May 2023

In light of the alarming results emerging from some studies and reports on the significant increase in aggressive online behaviors among children and adolescents during the COVID-19 pandemic, the current research aimed at providing a more detailed evaluation of the investigations focusing on the cyberbullying prevalence rates published between 2020 and 2023. To this purpose, systematic searches were conducted on four databases (Web of Science, APA PsycInfo, Scopus and Google Scholar), and following PRISMA guidelines, 16 studies were included and qualitatively reviewed. Although studies were characterized by a large variety in cyberbullying operationalization and measurement, and by different methodologies used for data collection, the prevalence rates of the involvement in cyberbullying and/or cybervictimization generally revealed opposite trends: an increase in many Asian countries and Australia and a decrease in Western countries. The findings were also discussed by considering the effects of the COVID-19 pandemic. Finally, some suggestions were provided to policy makers for promoting prevention and intervention anti-cyberbullying programs in school contexts.
Professionals’ Perspectives on the Effects of the COVID-19 Pandemic among Child and Adolescent Victims of Domestic Violence Living in the Portuguese Residential Foster Care System
Authors: Salgado Micaela , Neves Sofia , Silva Estefânia
Published: May 2023

The COVID-19 pandemic had several negative impacts on child and adolescent victims of domestic violence, especially on those who lived in the residential foster care system. The main goal of the present study was to understand these negative impacts through the perspectives of professionals in Portuguese residential foster care structures using both individual interviews and an online survey. One hundred and three professionals aged between 22 and 64 years (M = 38.39; SD = 8.34) participated in the online survey (86 females and 17 males). Of those, seven professionals, four females and three males aged between 29 and 49 years (M = 38.43, SD = 7.50), were also interviewed. According to the participants, the conditions imposed by the COVID-19 pandemic contributed adversely not only to the increase in domestic violence against children and adolescents but also to the aggravation of the conditions children and adolescents living in the Portuguese residential foster care system were exposed to, namely concerning family relationships, access to resources and services, and institutional dynamics. The results suggest the necessity to develop standard procedures to cope with pandemic situations in the residential foster care system.
How Do Physiotherapists Explain Influencing Factors to Chronic Low Back Pain? A Qualitative Study Using a Fictive Case of Chronic Non-Specific Low Back Pain
Authors: Vanderstraeten Rob , Fourré Antoine , Demeure Isaline , Demoulin Christophe , Michielsen Jozef , Anthierens Sibyl , Bastiaens Hilde , Roussel Nathalie
Published: May 2023

Background: While pain is influenced by multiple factors including psychosocial factors, previous research has shown that physiotherapists still favour a biomedical approach. Purpose: To evaluate: (1) how physiotherapists explain the patient’s chronic non-specific low back pain (LBP); (2) whether physiotherapists use one or multiple influencing factors, and (3) whether these factors are framed in a biopsychosocial or biomedical approach. Materials and methods: This exploratory qualitative study uses a vignette depicting chronic non-specific LBP and employs a flexible framework analysis. Physiotherapists were asked to mention contributing factors to the pain based on this vignette. Five themes were predefined (“Beliefs”, “Previous experiences”, “Emotions”, “Patients behaviour”, “Contextual factors”) and explored. Results: Physiotherapists use very brief explanations when reporting contributing factors to chronic pain (median 13 words). Out of 670 physiotherapists, only 40% mentioned more than two different themes and 2/3rds did not see any link between the patients’ misbeliefs and pain. Only a quarter of the participants mentioned the patient’s worries about pain and movement, which is considered to be an important influencing factor. Conclusion: The lack of a multifactorial approach and the persistent biomedical beliefs suggest that it remains a challenge for physiotherapists to fully integrate the biopsychosocial framework into their management of chronic LBP.
In Situ Construction of Nitrogen-Doped and Zinc-Confined Microporous Carbon Enabling Efficient Na+-Storage Abilities
Authors: Liao Wan-Ling , Abdelaal Mohamed M. , Amirtha Rene-Mary , Fang Chia-Chen , Yang Chun-Chen , Hung Tai-Feng
Published: May 2023

Benefiting from the additional active sites for sodium-ion (Na+) adsorption and porous architecture for electrolyte accessibility, nitrogen-doped porous carbon has been considered the alternative anode material for Na+-storage applications. In this study, nitrogen-doped and zinc-confined microporous carbon (N,Z-MPC) powders are successfully prepared by thermally pyrolyzing the polyhedral ZIF-8 nanoparticles under an argon atmosphere. Following the electrochemical measurements, the N,Z-MPC not only delivers good reversible capacity (423 mAh/g at 0.02 A/g) and comparable rate capability (104 mAh/g at 1.0 A/g) but also achieves a remarkable cyclability (capacity retention: 96.6% after 3000 cycles at 1.0 A/g). Those can be attributed to its intrinsic characteristics: (a) 67% of the disordered structure, (b) 0.38 nm of interplanar distance, (c) a great proportion of sp2-type carbon, (d) abundant microporosity, (e) 16.1% of nitrogen doping, and (f) existence of sodiophilic Zn species, synergistically enhancing the electrochemical performances. Accordingly, the findings observed here support the N,Z-MPC to be a potential anode material enabling exceptional Na+-storage abilities.
Evolution and Expression of the Meprin and TRAF Homology Domain-Containing Gene Family in Solanaceae
Authors: Dai Yangshuo , Ma Sirui , Guo Yixian , Zhang Xue , Liu Di , Gao Yan , Zhai Chendong , Chen Qinfang , Xiao Shi , Zhang Zhenfei , Yu Lujun
Published: May 2023

Meprin and TRAF homology (MATH)-domain-containing proteins are pivotal in modulating plant development and environmental stress responses. To date, members of the MATH gene family have been identified only in a few plant species, including Arabidopsis thaliana, Brassica rapa, maize, and rice, and the functions of this gene family in other economically important crops, especially the Solanaceae family, remain unclear. The present study identified and analyzed 58 MATH genes from three Solanaceae species, including tomato (Solanum lycopersicum), potato (Solanum tuberosum), and pepper (Capsicum annuum). Phylogenetic analysis and domain organization classified these MATH genes into four groups, consistent with those based on motif organization and gene structure. Synteny analysis found that segmental and tandem duplication might have contributed to MATH gene expansion in the tomato and the potato, respectively. Collinearity analysis revealed high conservation among Solanaceae MATH genes. Further cis-regulatory element prediction and gene expression analysis showed that Solanaceae MATH genes play essential roles during development and stress response. These findings provide a theoretical basis for other functional studies on Solanaceae MATH genes.
A Potential ABA Analog to Increase Drought Tolerance in Arabidopsis thaliana
Authors: Liu Ruiqi , Liang Guoyan , Gong Jiaxin , Wang Jiali , Zhang Yanjie , Hao Zhiqiang , Li Guanglin
Published: May 2023

Abscisic acid (ABA) plays an important role in the response of plants to drought stress. However, the chemical structure of ABA is unstable, which severely limits its application in agricultural production. Here, we report the identification of a small molecule compound of tetrazolium as an ABA analog (named SLG1) through virtual screening. SLG1 inhibits the seedling growth and promotes drought resistance of Arabidopsis thaliana with higher stability. Yeast two-hybrid and PP2C inhibition assays show that SLG1 acts as a potent activator of multiple ABA receptors in A. thaliana. Results of molecular docking and molecular dynamics show that SLG1 mainly binds to PYL2 and PYL3 through its tetrazolium group and the combination is stable. Together, these results demonstrate that SLG1, as an ABA analogue, protects A. thaliana from drought stress. Moreover, the newly identified tetrazolium group of SLG1 that binds to ABA receptors can be used as a new option for structural modification of ABA analogs.
Molecular Mechanisms of Cardiac Development and Disease
Authors: Wagner Nicole , Wagner Kay-Dietrich
Published: May 2023

During development, the heart is the first organ to form and function [...]
Ozone Therapy versus Hyaluronic Acid Injections for Pain Relief in Patients with Knee Osteoarthritis: Preliminary Findings on Molecular and Clinical Outcomes from a Randomized Controlled Trial
Authors: Sconza Cristiano , Di Matteo Berardo , Queirazza Paolo , Dina Arianna , Amenta Roberta , Respizzi Stefano , Massazza Giuseppe , Ammendolia Antonio , Kon Elizaveta , de Sire Alessandro
Published: May 2023

Ozone therapy (OT) is used for the treatment of multiple musculoskeletal disorders. In recent years, there has been a growing interest in its use for the treatment of osteoarthritis (OA). The aim of this double-blind randomized controlled trial was to evaluate the efficacy of OT compared with hyaluronic acid (HA) injections for pain relief in patients with knee OA. Patients with knee OA for at least three months were included and randomly assigned to receive three intra-articular injections of ozone or HA (once a week). Patients were assessed at baseline and at 1, 3, and 6 months after the injections for pain, stiffness, and function using the WOMAC LK 3.1, the NRS, and the KOOS questionnaire. Out of 55 patients assessed for eligibility, 52 participants were admitted to the study and randomly assigned into the 2 groups of treatment. During the study, eight patients dropped out. Thus, a total of 44 patients, reached the endpoint of the study at 6 months. Both Group A and B consisted of 22 patients. At 1-month follow-up after injections, both treatment groups improved statistically significantly from baseline in all outcomes measured. At 3 months, improvements remained similarly consistent for Group A and Group B. At 6-month follow-up, the outcomes were comparable between the 2 groups, showing only a worsening trend in pain. No significant differences were found between the two groups in pain scores. Both therapies have proven to be safe, with the few recorded adverse events being mild and self-limiting. OT has demonstrated similar results to HA injections, proving to be a safe approach with significant effects on pain control in patients affected by knee OA. Due to its anti-inflammatory and analgesic effects, ozone might be considered as a potential treatment for OA.
Acacia senegal Budmunchiamines as a Potential Adjuvant for Rejuvenating Phenicol Activities towards Escherichia coli-Resistant Strains
Authors: Dofini Magnini René , Pedinielli François , Vergalli Julia , Ouedraogo Noufou , Remy Simon , Hilou Adama , Brunel Jean-Michel , Pagès Jean-Marie , Davin-Regli Anne
Published: May 2023

The continuous emergence of bacterial resistance alters the activities of different antibiotic families and requires appropriate strategies to solve therapeutic impasses. Medicinal plants are an attractive source for researching alternative and original therapeutic molecules. In this study, the fractionation of natural extracts from A. senegal and the determination of antibacterial activities are associated with molecular networking and tandem mass spectrometry (MS/MS) data used to characterize active molecule(s). The activities of the combinations, which included various fractions plus an antibiotic, were investigated using the “chessboard” test. Bio-guided fractionation allowed the authors to obtain individually active or synergistic fractions with chloramphenicol activity. An LC-MS/MS analysis of the fraction of interest and molecular array reorganization showed that most identified compounds are Budmunchiamines (macrocyclic alkaloids). This study describes an interesting source of bioactive secondary metabolites structurally related to Budmunchiamines that are able to rejuvenate a significant chloramphenicol activity in strains that produce an AcrB efflux pump. They will pave the way for researching new active molecules for restoring the activity of antibiotics that are substrates of efflux pumps in enterobacterial-resistant strains.
Are Genetic Modifiers the Answer to Different Responses to Hydroxyurea Treatment?—A Pharmacogenetic Study in Sickle Cell Anemia Angolan Children
Authors: Ginete Catarina , Delgadinho Mariana , Santos Brígida , Pinto Vera , Silva Carina , Miranda Armandina , Brito Miguel
Published: May 2023

Sickle cell anemia (SCA) is an inherited disease affecting the hemoglobin that is particularly common in sub-Saharan Africa. Although monogenic, phenotypes are markedly heterogeneous in terms of severity and life span. Hydroxyurea is still the most common treatment for these patients, and the response to treatment is highly variable and seems to be an inherited trait. Therefore, identifying the variants that might predict hydroxyurea response is important for identifying patients who will have a poorer or non-response to treatment, and the ones that are more prone to suffer from severe side effects. In the present pharmacogenetic study, we analyzed the exons of 77 genes described in the literature as potentially associated with hydroxyurea metabolism in Angolan children treated with hydroxyurea and evaluated the drug response considering fetal hemoglobin levels, other hematological and biochemical parameters, hemolysis, number of vaso-occlusive crises and hospitalizations. Thirty variants were identified in 18 of those genes as possibly associated with drug response, five of them in gene DCHS2. Other polymorphisms in this gene were also associated with hematological, biochemical and clinical parameters. Further research examining the maximum tolerated dose and fixed dose with a larger sample size is necessary to corroborate these findings.
Isoquercitrin Attenuates Steatohepatitis by Inhibition of the Activated NLRP3 Inflammasome through HSP90
Authors: Ma Ji , Li Maoru , Yang Tingting , Deng Yang , Ding Yadong , Guo Tiantian , Shang Jing
Published: May 2023

Non-alcoholic fatty liver disease (NAFLD) is a chronic liver disease with a global prevalence of 25%. However, the medicines approved by the FDA or EMA are still not commercially available for the treatment of NAFLD. The NOD-like receptor thermal protein domain-associated protein 3 (NLRP3) inflammasome plays a crucial role in inflammatory responses, and the mechanisms related to steatohepatitis have been sufficiently clarified. NLRP3 has been widely evaluated as a potential target for multiple active agents in treating NAFLD. As a quercetin glycoside, isoquercitrin (IQ) has a broad inhibitory effect on oxidative stress, cancers, cardiovascular diseases, diabetes, and allergic reactions in vitro and in vivo. This study aimed to investigate the undercover mechanism of IQ in the treatment of NAFLD, particularly in anti-steatohepatitis, by suppressing the NLRP3 inflammasome. In this study, a methionine-choline-deficient induced steatohepatitis mice model was used to explore the effect of IQ on NAFLD treatment. Further mechanism exploration based on transcriptomics and molecular biology revealed that IQ inhibited the activated NLRP3 inflammasome by down-regulating the expression of heat shock protein 90 (HSP90) and suppressor of G-two allele of Skp1 (SGT1). In conclusion, IQ could alleviate NAFLD by inhibiting the activated NLRP3 inflammasome by suppressing the expression of HSP90.
Isolation of Vaginal Epithelial Cells: In Preparation of Autologous Vaginal Tissue Lining for Congenital Absence of Vagina Patients
Authors: Lih Yuan Too , Sulaiman Nadiah , Nur Azurah Abdul Ghani , Maarof Manira , Razali Rabiatul Adawiyah , Koh Benson , Ibrahim Roszita , Zainuddin Ani Amelia , Yazid Muhammad Dain
Published: May 2023

Infertility is a condition affecting women who are born with an underdeveloped or absent vagina, a birth defect known as congenital absence of the vagina. It is a rare disorder where the development of the Mullerian duct is obstructed by unidentified causes. The case is seldom reported due to the low prevalence and sparse epidemiology studies worldwide. A potential solution for the disorder is neovaginal creation with in vitro cultured vaginal mucosa. Limited studies have reported its application, but none are reproducible or specific regarding the established processes for acquiring vaginal epithelial cells from vaginal biopsies. These research gaps were adequately answered with an epidemiology study of inpatient details in Hospital Canselor Tuanku Muhriz, Malaysia, established methods and outcomes of vaginal tissue processing and isolation, and characterization of vaginal epithelial cells using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) and immunofluorescence assays. The reported evidence and speculation that the disorder arises because of a cellular transition event between epithelial and mesenchymal cells during the development of the Mullerian duct could be key in the creation of neovaginas using established culture procedures to improve surgical results and restore fertility.
Hypermucoviscous Carbapenem-Resistant Klebsiella pneumoniae ST25 Infect Human Intestinal Epithelial Cells and Induce Moderate Inflammation
Authors: Dentice Maidana Stefania , Elean Mariano , Fukuyama Kohtaro , Imamura Yoshiya , Albarracín Leonardo , Saha Sudeb , Suda Yoshihito , Kurata Shoichiro , Jure María Ángela , Kitazawa Haruki , Villena Julio
Published: May 2023

Klebsiella pneumoniae is an opportunistic pathogen that can produce moderate and severe infections in immunosuppressed hosts. In recent years, an increase in the isolation of hypermucoviscous carbapenem-resistant K. pneumoniae with sequence type 25 (ST25) in hospitals in Norwest Argentina was observed. This work aimed to study the virulence and inflammatory potential of two K. pneumoniae ST25 strains (LABACER01 and LABACER27) in the intestinal mucosa. The human intestinal Caco-2 cells were infected with the K. pneumoniae ST25 strains, and their adhesion and invasion rates and changes in the expression of tight junction and inflammatory factors genes were evaluated. ST25 strains were able to adhere and invade Caco-2 cells, reducing their viability. Furthermore, both strains reduced the expression of tight junction proteins (occludin, ZO-1, and claudin-5), altered permeability, and increased the expression of TGF-β and TLL1 and the inflammatory factors (COX-2, iNOS, MCP-1, IL-6, IL-8, and TNF-α) in Caco-2 cells. The inflammatory response induced by LABACER01 and LABACER27 was significantly lower than the one produced by LPS or other intestinal pathogens, including K. pneumoniae NTUH-K2044. No differences in virulence and inflammatory potential were found between LABACER01 and LABACER27. In line with these findings, no major differences between the strains were found when the comparative genomic analysis of virulence factors associated with intestinal infection/colonization was performed. This work is the first to demonstrate that hypermucoviscous carbapenem-resistant K. pneumoniae ST25 infects human intestinal epithelial cells and induces moderate inflammation.