bluuebunny/crossref_metadata_2025 huggingface dataset:
Transcription Factor ERF194 Modulates the Stress-Related Physiology to Enhance Drought Tolerance of Poplar
Authors: Huan Xuhui , Wang Xingqi , Zou Shengqiang , Zhao Kai , Han Youzhi , Wang Shengji
Published: January 2023

Drought is one of the main environmental factors limiting plant growth and development. The AP2/ERF transcription factor (TF) ERF194 play key roles in poplar growth and drought-stress tolerance. However, the physiological mechanism remains to be explored. In this study, the ERF194-overexpression (OX), suppressed-expression (RNA interference, RNAi), and non-transgenic (WT) poplar clone 717 were used to study the physiology role of ERF194 transcription factor in poplar growth and drought tolerance. Morphological and physiological methods were used to systematically analyze the growth status, antioxidant enzyme activity, malondialdehyde (MDA), soluble sugars, starch, and non-structural carbohydrate (NSC) contents of poplar. Results showed that, compared with WT, OX plants had decrease in plant height, internode length, and leaf area and increased number of fine roots under drought stress. In addition, OX had higher water potential, activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD), contents of chlorophyll, soluble sugar, starch, and NSC, implying that ERF194 positively regulates drought tolerance in poplar. The growth status of RNAi was similar to those of WT, but the relative water content and CAT activity of RNAi were lower than those of WT under drought treatment. Based on the transcriptome data, functional annotation and expression pattern analysis of differentially expressed genes were performed and further confirmed by RT-qPCR analysis. Gene ontology (GO) enrichment and gene expression pattern analysis indicated that overexpression of ERF194 upregulated the expression of oxidoreductases and metabolism-related genes such as POD and SOD. Detection of cis-acting elements in the promoters suggested that ERF194 may bind to these genes through MeJA-responsive elements, ABA-responsive elements, or elements involved in defense and stress responses. The above results show that ERF194 improved tolerance to drought stress in poplar by regulating its growth and physiological factors. This study provides a new idea for the role of ERF194 transcription factor in plant growth and drought-stress response.
Thermodynamic Signatures of Blood Plasma Proteome in Neurodegenerative Pathologies
Authors: Danailova Avgustina , Todinova Svetla , Gartcheva Lidia , Bogdanova Desislava , Zlatareva Elena , Kalaydzhiev Nikolay , Milanov Ivan , Krumova Sashka , Taneva Stefka G.
Published: January 2023

Discovery of diagnostic biomarkers for age-related neurodegenerative pathologies (NDDs) is essential for accurate diagnosis, following disease progression and drug development. Blood plasma and blood cells are important peripheral sources for NDDs’ biomarkers that, although present in lower concentrations than in cerebrospinal fluid, would allow noninvasive diagnostics. To identify new biomarkers for Parkinson’s disease (PD) and amyotrophic lateral sclerosis (ALS), in this work we have evaluated the modifications in the thermodynamic behavior of blood plasma proteome exploring differential scanning calorimetry. The plasma thermodynamics reflected the complexity and heterogeneity of the two pathologies. The unfolding temperature of the most abundant plasma protein albumin and the weighted average center of the calorimetric profile appeared as the two thermodynamic signatures that reflected modifications of the plasma proteome, i.e., strong thermal stabilization of albumin and plasma proteins’ interaction network, related to both pathologies. Based on those two signatures, both PD and ALS patients were stratified in two sets, except several cases with thermodynamic parameters that strongly differed from those of the calorimetric sets. Along with modifications of the plasma thermodynamic behavior, we found altered globulin levels in all PD and ALS patients’ plasma (higher level of α- and β-globulin fractions and lower level of γ-globulin fraction than the respective reference values) employing capillary electrophoresis. The presented results reveal the potential of calorimetry to indirectly identify NDDs’ biomarkers in blood plasma.
On the Photo-Eradication of Methicillin-Resistant Staphylococcus aureus Biofilm Using Methylene Blue
Authors: Maliszewska Irena , Zdubek Anna
Published: January 2023

This work compared the effectiveness of several Methylene Blue (MB)-based protocols for photo-eradication of biofilms formed on the surface of the glass and stainless steel discs by S. aureus MRSA isolates using a diode laser (λ = 665 nm; output power 40 mW; energy fluence was 189 J cm−2). The results obtained showed that MB alone, up to a concentration of 62.5 mgL−1, had limited photo-bactericidal activity. It was possible to enhance the activity of MB using two types of spherical gold nanoparticles of similar sizes, 15 ± 3 nm/20 ± 3 nm, but differing in the method of their synthesis and stabilization. The enhancement of the photodestruction effect was related to the increased production of hydroxyl radicals by the MB+gold nanoparticles mixture, and this mixture showed dark cytotoxicity against the cocci studied. Effective destruction (mortality above 99.9%) of the biofilms formed by MRSA isolates was also possible without the use of gold nanoparticles, but the concentration of MB had to be at least 125 mgL−1. A highly efficient protocol of photodestruction of biofilms, consisting of triple exposure of biofilms to laser light in the presence of MB alone, combined with the removal of dead bacteria protecting deep layers of pathogens against photosensitization, was also described.
Chromosomal Rearrangements and Chromothripsis: The Alternative End Generation Model
Authors: de Groot Daniel , Spanjaard Aldo , Hogenbirk Marc A. , Jacobs Heinz
Published: January 2023

Chromothripsis defines a genetic phenomenon where up to hundreds of clustered chromosomal rearrangements can arise in a single catastrophic event. The phenomenon is associated with cancer and congenital diseases. Most current models on the origin of chromothripsis suggest that prior to chromatin reshuffling numerous DNA double-strand breaks (DSBs) have to exist, i.e., chromosomal shattering precedes rearrangements. However, the preference of a DNA end to rearrange in a proximal accessible region led us to propose chromothripsis as the reaction product of successive chromatin rearrangements. We previously coined this process Alternative End Generation (AEG), where a single DSB with a repair-blocking end initiates a domino effect of rearrangements. Accordingly, chromothripsis is the end product of this domino reaction taking place in a single catastrophic event.
Strategies to Overcome Resistance to Immune-Based Therapies in Osteosarcoma
Authors: Hattinger Claudia Maria , Salaroglio Iris Chiara , Fantoni Leonardo , Godel Martina , Casotti Chiara , Kopecka Joanna , Scotlandi Katia , Ibrahim Toni , Riganti Chiara , Serra Massimo
Published: January 2023

Improving the prognosis and cure rate of HGOSs (high-grade osteosarcomas) is an absolute need. Immune-based treatment approaches have been increasingly taken into consideration, in particular for metastatic, relapsed and refractory HGOS patients, to ameliorate the clinical results currently achieved. This review is intended to give an overview on the immunotherapeutic treatments targeting, counteracting or exploiting the different immune cell compartments that are present in the HGOS tumor microenvironment. The principle at the basis of these strategies and the possible mechanisms that HGOS cells may use to escape these treatments are presented and discussed. Finally, a list of the currently ongoing immune-based trials in HGOS is provided, together with the results that have been obtained in recently completed clinical studies. The different strategies that are presently under investigation, which are generally aimed at abrogating the immune evasion of HGOS cells, will hopefully help to indicate new treatment protocols, leading to an improvement in the prognosis of patients with this tumor.
Sensory Receptor, Inflammatory, and Apoptotic Protein Expression in the Bladder Urothelium of Patients with Different Subtypes of Interstitial Cystitis/Bladder Pain Syndrome
Authors: Jiang Yuan-Hong , Jhang Jia-Fong , Birder Lori A. , Kuo Hann-Chorng
Published: January 2023

The aim of this study was to investigate the expression levels of sensory receptors, inflammatory proteins, and pro-apoptotic proteins in the urothelium of non-Hunner’s interstitial cystitis (NHIC) bladders of patients with different clinical and cystoscopic phenotypes. The urothelia from the bladders of 52 NHIC patients were harvested. The expression of sensory receptors, including TRPV1, TRPV4, TRPA1, H1-receptors, and sigma-1 receptors; the inflammatory proteins p38 and tryptase; and the pro-apoptotic proteins, such as caspase-3, BAD, and BAX in the urothelium, were investigated using immunohistochemistry and Western blotting. We compared the expression levels of these proteins in NHIC subtypes according to IC symptom scores, visual analog scores of bladder pain, maximal bladder capacity, glomerulation grades, and combined maximal bladder capacity and glomerulations after cystoscopic hydrodistention. The expression levels of TRPV1, TRPV4, sigma-1, P38, tryptase, caspase-3, and BAD were significantly increased in the urothelium of IC/BPS patients compared with the expression levels in the controls. TRPV1 was significantly associated with IC symptom severity. However, no significant differences in sensory receptor expression in the IC/BPS bladders with different bladder conditions were detected. Inflammatory and pro-apoptotic protein expression levels in the urothelium were similar among the IC/BPS subgroups. This study concluded that IC/BPS patients with frequency and bladder pain complaints have higher levels of urothelial sensory receptors, and inflammatory and pro-apoptotic proteins. The expression levels of these sensory receptors, inflammatory proteins, and pro-apoptotic proteins are not significantly different among IC/BPS bladders with different conditions.
Triggering of Polymer-Degrading Enzymes from Layered Double Hydroxides for Recycling Strategies
Authors: Romano Angela , Rosato Antonella , Bianchi Stefano , Zanaroli Giulio , Celli Annamaria , Totaro Grazia , Sisti Laura
Published: January 2023

The use of degrading enzymes in polymer formulation is a very attractive strategy to manage the end-of-life of plastics. However, high temperatures cause the denaturation of enzymes and the loss of their catalytic activity; therefore, protection strategies are necessary. Once protected, the enzyme needs to be released in appropriate media to exert its catalytic activity. A successful protection strategy involves the use of layered double hydroxides: cutinase, selected as a highly degrading polyester hydrolytic enzyme, is thermally protected by immobilization in Mg/Al layered double hydroxide structures. Different triggering media are here evaluated in order to find the best releasing conditions of cutinase from LDH. In detail, phosphate and citrate–phosphate buffers, potassium carbonate, sodium chloride, and sodium sulfate solutions are studied. After the comparison of all media in terms of protein release and activity retained, phosphate buffer is selected as the best candidate for the release of cutinase from LDH, and the effect of pH and concentration is also evaluated. The amount of the enzyme released is determined with the Lowry method. Activity tests are performed via spectrophotometry.
Clinical Use of Gastric Antisecretory Drugs in Hospitalized Pediatric Patients
Authors: Locci Cristian , Cuzzolin Laura , Cheri Gianluca , Saderi Laura , Sotgiu Giovanni , Antonucci Roberto
Published: January 2023

Antisecretory drugs are frequently used in the treatment of pediatric gastrointestinal disorders. This study was aimed to assess the prescribing patterns and the safety of ranitidine and proton pump inhibitors (PPIs) in a cohort of Italian pediatric patients. Children aged >1 month to <16 years that were admitted to our Pediatric Clinic between 2016 and 2018 were enrolled in this retrospective observational study. All data were obtained from medical records and a parent telephone questionnaire. The exclusion criteria included the use of antisecretory therapy at hospital admission, failure to collect the relevant clinical data, and failure to administer the questionnaire. This study included 461 subjects, who were divided into four age groups: <2 years, 2–5 years, 6–11 years, and ≥12 years. Ranitidine was prescribed in 396 (85.9%) patients, mainly for the acute treatment of gastrointestinal symptoms, and a PPI was given to 65 (14.1%) children to treat gastroesophageal reflux disease, gastritis/ulcer, or for gastroprotection. During the study period, the percentage of patients treated with ranitidine progressively increased, except in the 2–5-year age group. We observed eighty-seven adverse drug reactions (ADRs), 61 of which occurred in the ranitidine group and 26 in the PPI group. The most common ADR was constipation (n = 35), which occurred more frequently in children treated with PPIs and in the 6–11-year age group. Ranitidine was the most used antisecretory drug in all the age groups, especially for acute treatment. Conversely, PPIs were the drugs of choice for prolonged treatments. Further research should be focused on developing an effective and safer alternative to ranitidine.
Cutting-Edge Approaches in Respiratory and Critical Care Medicine
Authors: Pantazopoulos Ioannis , Kotsiou Ourania S.
Published: January 2023

The COVID-19 pandemic has affected health care across the world, with respiratory and critical care medicine being affected the most [...]
Exercise-Induced Pulmonary Hypertension: A Valid Entity or Another Factor of Confusion?
Authors: Khattab Elina , Velidakis Nikolaos , Gkougkoudi Evaggelia , Kadoglou Nikolaos P.E.
Published: January 2023

Exercise-induced pulmonary hypertension EIPH has been defined as an increase in mean pulmonary arterial pressure (mPAP) during exercise in otherwise normal values at rest. EIPH reflects heart and/or lung dysfunction and may precede the development of manifest pulmonary hypertension (PH) in a proportion of patients. It is also associated with decreased life expectancy in patients with heart failure with reduced ejection fraction (HFrEF) or left ventricle (LV) valvular diseases. Diastolic dysfunction exacerbated during exercise relates to increased LV filling pressure and left atrial pressure (LAP). In this context backward, transmitted pressure alone or accompanied with backward blood flow promotes EIPH. The gold standard of EIPH assessment remains the right heart catheterization during exercise, which is an accurate but invasive method. Alternatively, non-invasive diagnostic modalities include exercise stress echocardiography (ESE) and cardiopulmonary exercise testing (CPET). Both diagnostic tests are performed under gradually increasing physical stress using treadmill and ergo-cycling protocols. Escalating workload during the exercise is analogous to the physiological response to real exercise. The results of the latter techniques show good correlation with invasive measurements, but they suffer from lack of validation and cut-off value determination. Although it is not officially recommended, there are accumulated data supporting the importance of EIPH diagnosis in the assessment of other mild/subclinical or probably fatal diseases in patients with latent PH or heart failure or LV valvular disease, respectively. Nevertheless, larger, prospective studies are required to ensure its role in clinical practice.
Work Hardening Behavior and Microstructure Evolution of a Cu-Ti-Cr-Mg Alloy during Room Temperature and Cryogenic Rolling
Authors: Li Rong , Xiao Zhu , Li Zhou , Meng Xiangpeng , Wang Xu
Published: January 2023

A Cu-1.79Ti-0.39Cr-0.1Mg (wt.%) alloy was prepared by a vacuum induction melting furnace in a high-purity argon atmosphere. The effects of room temperature rolling and cryogenic rolling on the microstructure, textures, and mechanical properties of the alloy were investigated by means of electron backscatter diffraction, transmission electron microscopy, and X-ray diffraction. The results show that the hardness of the cryogenically rolled alloy is 18–30 HV higher than that of the room temperature rolled alloy at any tested rolling reduction. The yield strength and tensile strength of the alloy cryogenically rolled by 90% reduction are 723 MPa and 796 MPa, respectively. With the increase of rolling reduction, the orientation density of the Cube texture decreases, while the Brass texture increases. The Brass texture is preferred especially during the cryogenic rolling, suggesting that the cross-slip is inhibited at the cryogenic temperature. The dislocation densities of Cu-Ti-Cr-Mg alloy increase significantly during the deformation, finally reaching 23.03 × 10−14 m−2 and 29.98 × 10−14 m−2 after a 90% reduction for the room temperature rolled and cryogenically rolled alloys, respectively. This difference could be attributed to the impediment effect of cryogenic temperature on dynamic recovery and dynamic recrystallization. The cryogenic temperature promotes the formation of the dislocation and the nano-twins, leading to the improvement of the mechanical properties of the alloy.
Surface Morphology and Subsurface Microstructure Evolution When Form Grinding 20Cr2Ni4A Alloys
Authors: Zhang Xiaodong , Jiang Xiaoyang , Li Maojun , Gong Pan
Published: January 2023

20Cr2Ni4A alloy is widely used in the manufacturing of heavy-duty gears, although limited information about its machinability during the form-grinding process has been reported. In this work, form-grinding trials on transmission gears of 20Cr2Ni4A alloy under various parameters were conducted. Surface morphology of the gear tooth, surface roughness distribution and microstructure evolution of the machined surface layer were comprehensively studied, and the influence of grinding parameters on grinding performance was investigated. The formation mechanisms of surface/subsurface defects during the form-grinding process, including plastic flow, deep grooves, successive crushing zone, adhesive chips and cavities, were analyzed. Results showed that the change in contact conditions between the grinding wheel and tooth surface led to the decrease in the surface roughness from tooth tip to root. Mechanical force and grinding heat promoted the deformation and refinement of the microstructure within the machined surface layer. With the increase in cutting depth and feed speed, the deformation ratio of the microstructure increased, which was also consistent with the variation trend in the form-grinding temperature.
Microstructural Characterization of the As-Cast and Homogenized Al-Cu-Mg-Ag Alloy
Authors: Lin Haitao , Zhu Kai , Liu Qilong , Chen Lifang , Wang Zhengan , Li Xiwu
Published: January 2023

In this study, the as-cast microstructure and the evolution of the homogenized microstructure of large-scale industrialized Al-Cu-Mg-Ag heat-resistant aluminum alloy ingots were investigated by means of optical microscopy (OM), scanning electron microscopy (SEM), energy dispersive analysis (EDS), and differential scanning calorimetry (DSC). The results indicate that the dendritic segregation is evident in the ingot along the radial direction, and the grain boundaries are decorated with lots of net-shaped continuous eutectic structures. With the homogenization time extension and the homogenization temperature increase, the eutectic phases (i.e., the primary Al2Cu phase, the Al2CuMg phase, and the AlCuMgAg quaternary phase) at the grain boundaries gradually dissolve back into the matrix. Meanwhile, most of the dendritic grain boundaries gradually become sparse and thinner. Finally, it is found that the optimal homogenization regime of the Al-Cu-Mg-Ag alloy is 420 °C/5 h+480 °C/8 h+515 °C/24 h.
Design of Real-Time Extremum-Seeking Controller-Based Modelling for Optimizing MRR in Low Power EDM
Authors: Ismail Mohamed Rabik Mohamed , Thangaraj Muthuramalingam , Karmiris-Obratański Panagiotis , Papazoglou Emmanouil , Karkalos Nikolaos
Published: January 2023

Electric discharge machining (EDM) is one of the non-conventional machining processes that supports machining for high-strength and wear-resistant materials. It is a challenging task to select the process parameters in real-time to maximize the material removal rate since real-time process trials are expensive and the EDM process is stochastic. For the ease of finding process parameters, a modelling of the EDM process is proposed. Due to the non-linear relationship between the material removal rate (MRR) and discharge time, a model-free adaptive extremum-seeking controller (ESC) is proposed in the feedback path of the EDM process for finding an optimal value of the discharge time at which the maximum material removal rate can be achieved. The results of the model show a performance that is closer to the actual process by choosing steel workpieces and copper electrodes. The proposed model offers a lower error rate when compared with actual experimental process data. When compared to manual searching for an optimal point, extreme seeking online searching performed better as per the experimental results. It was observed that the experimental validation also proved that the ESC can produce a large MRR by tracking the extremum control. The present study has been limited to only the MRR, but it is also possible to implement such algorithms for more than one response parameter optimization in future studies. In such cases the performance measures of the process could be further enhanced, which could be used for a real-time complex die- and mold-making process using EDM.
Dyeable Hydrophilic Surface Modification for PTFE Substrates by Surface Fluorination
Authors: Kobayashi Mizuki , Nishimura Fumihiro , Kim Jae-Ho , Yonezawa Susumu
Published: January 2023

Polytetrafluoroethylene (PTFE) is the most widely used fluoropolymer that has various functionalities such as heat resistance, chemical resistance, abrasion resistance, and non-adhesiveness. However, PTFE is difficult to dye because of its high water repellency. In this study, the PTFE surface was modified by a combination of gold sputtering and surface fluorination to improve dyeability. X-ray photoelectron spectroscopy indicated that, compared with the untreated sample, the gold-sputtered and acid-washed surface of PTFE had a negligible number of C–F terminals. Furthermore, the intensity of the C–C peak increased drastically. The polar groups (C=O and C–Fx) increased after surface fluorination, which enhanced the electronegativity of the surface according to the zeta potential results. Dyeing tests with methylene blue basic dye showed that the dye staining intensity on the surface of fluorinated PTFE samples was superior to other samples. It is due to the increased surface roughness and the negatively charged surface of fluorinated PTFE samples. The modified PTFE substrates may find broad applicability for dyeing, hydrophilic membrane filters, and other adsorption needs.
Integration of Ultra- and Nanofiltration for Potato Processing Water (PPW) Treatment in a Circular Water Recovery System
Authors: Rajewska Paulina , Janiszewska Jolanta , Rajewski Jakub
Published: January 2023

The article analyzes integrated ultrafiltration (UF) and nanofiltration (NF) processes for potato processing wastewater treatment for the purpose of which a laboratory filtration system for flat sheet membranes with the effective surface area of 1.4 × 10−2 m2 (UF: polysulfone, cut-off: 10,000 Da; NF: polypiperazine amide, cut-off: 150–300 Da) was used. As part of the study, the effect of the transmembrane pressure of UF (0.2 MPa and 0.4 MPa) and NF (1.0 MPa and 1.8 MPa) on the permeate flux and rejection coefficient was investigated and the impact of sewage preparation methods on the degree of pollution reduction was determined. Moreover, a method for a fouling layer removal from the UF membranes is also proposed. The results of the analyses conducted by the authors show that the pretreatment stage offers additional advantages to TSS and turbidity removal. In both cases (0.2 and 0.4 MPa), UF used after the pretreatment process resulted in a 97–99% reduction in these impurities. The analysis of the determined rejection coefficients shows that the use of NaOH and H2O2 for the regeneration of the UF membrane has a positive effect on filtration efficiency. Regarding NF, the rejection coefficients for most tested parameters were higher for the 1.8 MPa process compared to 1.0 MPa, and approximately 80% of water was recovered.
Some Lessons Learned on the Impact of the Storage Conditions, Syringe Wash Solvent, and the Way of GC-MS Injection on the Reproducibility of Metabolomic Studies
Authors: Kurbatov Ilya , Kiseleva Olga , Arzumanian Viktoriia , Dolgalev Georgii , Poverennaya Ekaterina
Published: January 2023

Metabolomics based on two-dimensional gas chromatography coupled with mass spectrometry is making high demands on accuracy at all stages of sample preparation, up to the storage and injection into the analytical system. In high sample flow conditions, good repeatability in peak areas and a list of detectable metabolites is sometimes challenging to obtain. In this research, we successfully obtained good repeatability for the peak areas of MSFTA-derivatives of 29 core blood plasma metabolites. Six different strategies of storage and injection were investigated and evaluated for the reproducibility of the obtained data. As the essential factors, we considered popular GC-MS syringe washing solvents (methanol and pyridine); storage conditions (freshly prepared samples and stored for 24 h in ambient temperature or in the refrigerator); scheme of injection (one injection per intact vial or three sequential injections per vial). Our GC×GC-MS results demonstrated that the usage of pyridine as a syringe wash solvent and triple injecting the sample from the same vial was the most appropriate for minimizing the coefficient of variation (CV) of the results obtained (in general, <10%). The prolonged storage of samples does not have a noticeable effect on the change in the areas of chromatographic peaks of metabolites, although it reduces CV in some cases. These storage and injection recommendations can be used in future study protocols for the GC×GC-MS analysis of blood plasma.
Two-Dimensional Omnidirectional Wind Energy Harvester with a Cylindrical Piezoelectric Composite Cantilever
Authors: Xin Mingyong , Jiang Xueling , Xu Changbao , Yang Jing , Lu Caijiang
Published: January 2023

To improve the response-ability of the energy harvester to multidirectional wind, this paper proposes a wind energy harvester to scavenge wind-induced vibration energy. The harvester comprises a cylindrical beam instead of conventional thin rectangular cantilevers, a bluff body (square prism or circle cylinder), and a piezoelectric tube bonded to the bottom side of the beam for energy conversion. Benefiting from the symmetry of the cylindrical structure, this harvester can respond to airflow from every direction of the two-dimensional plane. The performance of the harvester under a wind speed range of 1.5–8 m/s has been tested. The results demonstrate that the proposed harvester can respond to the wind from all directions of the two-dimensional plane. It provides a direction for the future in-depth study of multidirectional wind energy harvesting.
Acoustic Wake-Up Technology for Microsystems: A Review
Authors: Yang Deng , Zhao Jiahao
Published: January 2023

Microsystems with capabilities of acoustic signal perception and recognition are widely used in unattended monitoring applications. In order to realize long-term and large-scale monitoring, microsystems with ultra-low power consumption are always required. Acoustic wake-up is one of the solutions to effectively reduce the power consumption of microsystems, especially for monitoring sparse events. This paper presents a review of acoustic wake-up technologies for microsystems. Acoustic sensing, acoustic recognition, and system working mode switching are the basis for constructing acoustic wake-up microsystems. First, state-of-the-art MEMS acoustic transducers suitable for acoustic wake-up microsystems are investigated, including MEMS microphones, MEMS hydrophones, and MEMS acoustic switches. Acoustic transducers with low power consumption, high sensitivity, low noise, and small size are attributes needed by the acoustic wake-up microsystem. Next, acoustic features and acoustic classification algorithms for target and event recognition are studied and summarized. More acoustic features and more computation are generally required to achieve better recognition performance while consuming more power. After that, four different system wake-up architectures are summarized. Acoustic wake-up microsystems with absolutely zero power consumption in sleep mode can be realized in the architecture of zero-power recognition and zero-power sleep. Applications of acoustic wake-up microsystems are then elaborated, which are closely related to scientific research and our daily life. Finally, challenges and future research directions of acoustic wake-up microsystems are elaborated. With breakthroughs in software and hardware technologies, acoustic wake-up microsystems can be deployed for ultra-long-term and ultra-large-scale use in various fields, and play important roles in the Internet of Things.
Geochronology, Petrogenesis and Geodynamic Setting of the Kaimuqi Mafic–Ultramafic and Dioritic Intrusions in the Eastern Kunlun Orogen, NW China
Authors: Fan Dongxu , Tan Shucheng , Wang Xia , Qin Zeli , Zhao Junfang , Yang Le , Zhang Wanhui , Li Xiaoliang , Yan Zhengping , Yang Guizhong , Li Liang
Published: January 2023

The Kaimuqi area in the Eastern Kunlun Orogen (EKO) contains many lherzolite, olivine websterite, gabbro and diorite intrusions, and new zircon U–Pb dating, Lu–Hf isotope and whole-rock geochemical data are presented herein to further confirm the Late Triassic mafic–ultramafic magmatism with Cu–Ni mineralization and to discuss the petrogenesis and geodynamic setting. Zircon U–Pb dating shows that the Late Triassic ages, corresponding to 220 Ma and 222 Ma, reveal the mafic–ultramafic and dioritic magmatism in Kaimuqi, respectively. Zircon from gabbro has εHf(t) values of −3.4 to −0.2, with corresponding TDM1 ages of 994–863 Ma. The mafic–ultramafic rocks generally have low SiO2, (Na2O+K2O) and TiO2 contents and high MgO contents and Mg# values. They are relatively enriched in light rare earth elements (LREEs) and large ion lithophile elements (LILEs) and depleted in heavy REEs (HREEs) and high-field-strength elements (HFSEs), indicating that the primary magma was derived from the metasomatized lithospheric mantle. The diorites show sanukitic high-Mg andesite properties (e.g., MgO = 2.78%–3.54%, Mg# = 50–55, Cr = 49.6–60.0 ppm, Sr = 488–512 ppm, Y = 19.6–21.8 ppm, Ba = 583–722 ppm, Sr/Y = 23.5–25.4, K/Rb = 190–202 and Eu/Eu* = 0.73–0.79), with LREEs and LILEs enrichments and HREEs and HFSEs depletions. We suggest that the primary Kaimuqi diorite magma originated from enriched lithospheric mantle that was metasomatized by subduction-derived fluids and sediments. The Kaimuqi mafic–ultramafic and dioritic intrusions, with many other mafic–ultramafic and K-rich granitic/rhyolitic rocks in the EKO, formed in a dynamic extensional setting after the Palaeo-Tethys Ocean closure.
Catalpol Attenuates Oxidative Stress and Inflammation via Mechanisms Involving Sirtuin-1 Activation and NF-κB Inhibition in Experimentally-Induced Chronic Kidney Disease
Authors: Zaaba Nur Elena , Al-Salam Suhail , Beegam Sumaya , Elzaki Ozaz , Yasin Javed , Nemmar Abderrahim
Published: January 2023

Chronic kidney disease (CKD) is a stealthy disease, and its development is linked to mechanisms including inflammation and oxidative stress. Catalpol (CAT), an iridoid glucoside from the root of Rehmannia glutinosa, is reported to manifest anti-inflammatory, antioxidant, antiapoptotic and antifibrotic properties. Hence, we studied the possible nephroprotective effects of CAT and its mechanisms in an adenine-induced (0.2% w/w in feed for 4 weeks) murine model of CKD by administering 5 mg/kg CAT to BALB/c mice for the duration of 4 weeks except during weekends. Upon sacrifice, the kidney, plasma and urine were collected and various physiological, biochemical and histological endpoints were assessed. CAT significantly ameliorated the adenine-induced altered body and kidney weight, water intake, urine volume, and concentrations of urea and creatinine in plasma, as well as the creatinine clearance and the albumin and creatinine ratio. Moreover, CAT significantly ameliorated the effect of adenine-induced kidney injury by reducing the kidney injury molecule-1, neutrophil gelatinase-associated lipocalin, cystatin C and adiponectin. Similarly, the augmented concentrations of markers of inflammation and oxidative stress in the adenine-treated group were markedly reduced with CAT pretreatment. Furthermore, CAT prevented adenine-induced deoxyribonucleic acid damage and apoptotic activity in the kidneys. Histologically, CAT significantly reduced the formation of tubular necrosis and dilation, as well as interstitial fibrosis in the kidney. In addition to that, CAT significantly decreased the adenine-induced increase in the phosphorylated NF-κB and reversed the reduced expression of sirtuin-1 in the kidney. In conclusion, CAT exhibits salutary effects against adenine-induced CKD in mice by mitigating inflammation, oxidative stress and fibrosis via mechanisms involving sirtuin-1 activation and NF-κB inhibition. Confirmatory studies are warranted in order to consider CAT as a potent nephroprotective agent against CKD.
Solubility Enhanced Formulation Approaches to Overcome Oral Delivery Obstacles of PROTACs
Authors: Pöstges Florian , Kayser Kevin , Appelhaus Jan , Monschke Marius , Gütschow Michael , Steinebach Christian , Wagner Karl G.
Published: January 2023

PROteolysis TArgeting Chimaeras (PROTACs) offer new opportunities in modern medicine by targeting proteins that are undruggable to classic inhibitors. However, due to their hydrophobic structure, PROTACs typically suffer from low solubility, and oral bioavailability remains challenging. At the same time, due to their investigative state, the drug supply is meager, leading to limited possibilities in terms of formulation development. Therefore, we investigated the solubility enhancement employing mini-scale formulations of amorphous solid dispersions (ASDs) and liquisolid formulations of the prototypic PROTAC ARCC-4. Based on preliminary supersaturation testing, HPMCAS (L Grade) and Eudragit® L 100-55 (EL 100-55) were demonstrated to be suitable polymers for supersaturation stabilization of ARCC-4. These two polymers were selected for preparing ASDs via vacuum compression molding (VCM), using drug loads of 10 and 20%, respectively. The ASDs were subsequently characterized with respect to their solid state via differential scanning calorimetry (DSC). Non-sink dissolution testing revealed that the physical mixtures (PMs) did not improve dissolution. At the same time, all ASDs enabled pronounced supersaturation of ARCC-4 without precipitation for the entire dissolution period. In contrast, liquisolid formulations failed in increasing ARCC-4 solubility. Hence, we demonstrated that ASD formation is a promising principle to overcome the low solubility of PROTACs.
Creating a Microenvironment to Give Wings to Dental Pulp Regeneration—Bioactive Scaffolds
Authors: Hu Nan , Li Weiping , Jiang Wentao , Wen Jin , Gu Shensheng
Published: January 2023

Dental pulp and periapical diseases make patients suffer from acute pain and economic loss. Although root canal therapies, as demonstrated through evidence-based medicine, can relieve symptoms and are commonly employed by dentists, it is still difficult to fully restore a dental pulp’s nutrition, sensory, and immune-regulation functions. In recent years, researchers have made significant progress in tissue engineering to regenerate dental pulp in a desired microenvironment. With breakthroughs in regenerative medicine and material science, bioactive scaffolds play a pivotal role in creating a suitable microenvironment for cell survival, proliferation, and differentiation, following dental restoration and regeneration. This article focuses on current challenges and novel perspectives about bioactive scaffolds in creating a microenvironment to promote dental pulp regeneration. We hope our readers will gain a deeper understanding and new inspiration of dental pulp regeneration through our summary.
Enhancing the Production of Hydrolytic Enzymes in Elicited Tamarillo (Solanum betaceum Cav.) Cell Suspension Cultures
Authors: Casimiro Bruno , Mota Inês , Veríssimo Paula , Canhoto Jorge , Correia Sandra
Published: January 2023

Plant cell suspension cultures are widely used as a tool for analyzing cellular and molecular processes, metabolite synthesis, and differentiation, bypassing the structural complexity of plants. Within the range of approaches used to increase the production of metabolites by plant cells, one of the most recurrent is applying elicitors capable of stimulating metabolic pathways related to defense mechanisms. Previous proteomics analysis of tamarillo cell lines and cell suspension cultures have been used to further characterize and optimize the growth and stress-related metabolite production under in vitro controlled conditions. The main objective of this work was to develop a novel plant-based bioreactor system to produce hydrolytic enzymes using an elicitation approach. Based on effective protocols for tamarillo micropropagation and plant cell suspension culture establishment from induced callus lines, cell growth has been optimized, and enzymatic activity profiles under in vitro controlled conditions characterized. By testing different sucrose concentrations and the effects of two types of biotic elicitors, it was found that 3% (w/v) sucrose concentration in the liquid medium enhanced the production of hydrolytic enzymes. Moreover, casein hydrolysate at 0.5 and 1.5 g/L promoted protein production, whereas yeast extract (0.5 g/L) enhanced glycosidase activity. Meanwhile, chitosan (0.05 and 0.1 g/L) enhanced glycosidases, alkaline phosphates, and protease activities.
The Influence on Fracture Resistance of Different Composite Resins and Prefabricated Posts to Restore Endodontically Treated Teeth
Authors: Pamato Saulo , Ricci Weber Adad , Kuga Milton Carlos , de Oliveira Eliane Cristina Gulin , Moraes João Carlos Silos , Só Marcus Vinicius Reis , Trevisan Tamara Carolina , Júnior Newton Fahl , Pereira Jefferson Ricardo
Published: January 2023

Recent formulations of resin-based composites have incorporated different combinations of materials. However, the mechanical and bonding behavior of these materials with intraradicular posts are unclear. This study aimed to evaluate the effect of light-cure and dual-cure resin composite posts on the fracture resistance of endodontically-treated teeth. Materials and Methods: Ninety extracted human upper canines were selected and randomly divided into nine groups (n=10): (G1) endodontically treated teeth without endodontic posts; (G2) glass-fiber post cemented with glass-ionomer cement; (G3) endodontic post by dual-cure composite resin (Rebilda DC); (G4) endodontic post by dual-cure composite resin (Cosmecore); (G5) endodontic post by dual-cure composite resin (Bis-Core); (G6) endodontic post by light-cure composite resin; (G7) glass-fiber post customized with flowable composite resin; (G8) glass-fiber post cemented with light-cure composite resin; (G9) glass-fiber post cemented with self-adhesive resin cement. After the post insertion, all specimens were subjected to mechanical (250,000 cycles) and thermocycling (6000 cycles, 5 °C/55 °C) and immediate loading at 45 degrees in a universal testing machine until fracture. The data were analyzed by one-way ANOVA and multiple comparisons using the Fisher LSD Method (p < 0 05). Results: The mean failure loads (±SD) for the groups ranged from 100.7 ± 22.6 N to 221.9 ± 48.9 N. The G1 group (without endodontic posts) had a higher fracture strength than all experimental groups (p < 0.001). Conclusions: Within the limitations, the light- and dual-cure post technique did not present lower fracture resistance values as compared to the conventional glass-fiber post.
Behavior Contracts in Psychiatric Practice and Everyday Situations: A Psychological and Psychiatric Viewpoint
Authors: Ino Hiroyasu , Nakazawa Eisuke , Akabayashi Akira
Published: January 2023

In recent years, “difficult” patients have gained attention, and behavior contracts have been introduced into clinical practice. This is because some patients behave inappropriately toward healthcare providers, and behavior contracts require patients to refrain from such behavior. However, it has been highlighted that behavior contracts have ethical problems. We present an ethical analysis of behavior contracts from the viewpoint of psychiatric practice and patient psychology. We analyze why patients become “difficult” for medical practitioners and explain why consideration must be given to the psychological aspects of the patient and the burden of mental illness. Behavior contracts are inappropriate because they do not consider individual patients’ psychological or psychiatric conditions and are applied uniformly. Moreover, the behavioral model that behavior contracts assume is not justified by today’s psychiatry. Furthermore, in this article, we show how behavior contracts promote the stigmatization of mental illness. For these reasons, we argue that the use of behavior contracts in clinical practice is not ethically justified. However, we add that physical violence against healthcare providers should not be tolerated under any circumstances.
An Improved TDOA Method for Land-Based Long-Range HF Skywave Source Geolocation and Experimental Results
Authors: Xu Chen , Cai Hongtao , Gao Shunzu , Zhai Qingwei
Published: January 2023

The real-time information of the unknown ionospheric environments is difficult to obtain, plaguing the timely and accurate geolocation of high frequency (HF) sources. In this paper, we propose an improved HF skywave source geolocation method based on the time-difference-of-arrival (TDOA) with the semidefinite programming (SDP), and model HF signal propagation paths as paths with significant non-line-of-sight (NLOS) biases. With this method, no priori information about the ionosphere, especially the priori ionospheric virtual heights of reflection, is necessary while timely and accurately geolocating the HF sources. Furthermore, we use the ray tracing technique and build a 3D ionospheric electron density gridded matrix model to simulate realistic HF signal propagation paths. In the simulations, the proposed method is compared with existing methods, and detailed geolocation error distribution maps are given. In the experiments, HF I/Q data captured from different types of HF transmitters are located by six receivers with time synchronization. Simulated and experimental results show that the proposed method improves the positioning accuracy by about 50% compared with existing methods under the same conditions, and the average relative positioning error is less than 2.7%.
Ensemble of Heterogeneous Base Classifiers for Human Gait Recognition
Authors: Derlatka Marcin , Borowska Marta
Published: January 2023

Human gait recognition is one of the most interesting issues within the subject of behavioral biometrics. The most significant problems connected with the practical application of biometric systems include their accuracy as well as the speed at which they operate, understood both as the time needed to recognize a particular person as well as the time necessary to create and train a biometric system. The present study made use of an ensemble of heterogeneous base classifiers to address these issues. A Heterogeneous ensemble is a group of classification models trained using various algorithms and combined to output an effective recognition A group of parameters identified on the basis of ground reaction forces was accepted as input signals. The proposed solution was tested on a sample of 322 people (5980 gait cycles). Results concerning the accuracy of recognition (meaning the Correct Classification Rate quality at 99.65%), as well as operation time (meaning the time of model construction at <12.5 min and the time needed to recognize a person at <0.1 s), should be considered as very good and exceed in quality other methods so far described in the literature.
Mapping Understory Vegetation Density in Mediterranean Forests: Insights from Airborne and Terrestrial Laser Scanning Integration
Authors: Ferrara Carlotta , Puletti Nicola , Guasti Matteo , Scotti Roberto
Published: January 2023

The understory is an essential ecological and structural component of forest ecosystems. The lack of efficient, accurate, and objective methods for evaluating and quantifying the spatial spread of understory characteristics over large areas is a challenge for forest planning and management, with specific regard to biodiversity and habitat governance. In this study, we used terrestrial and airborne laser scanning (TLS and ALS) data to characterize understory in a European beech and black pine forest in Italy. First, we linked understory structural features derived from traditional field measurements with TLS metrics, then, we related such metrics to the ones derived from ALS. Results indicate that (i) the upper understory density (5–10 m above ground) is significantly associated with two ALS metrics, specifically the mean height of points belonging to the lower third of the ALS point cloud within the voxel (HM1/3) and the corresponding standard deviation (SD1/3), while (ii) for the lower understory layer (2–5 m above ground), the most related metric is HM1/3 alone. As an example application, we have produced a map of forest understory for each layer, extending over the entire study region covered by ALS data, based on the developed spatial prediction models. With this study, we also demonstrated the power of hand-held mobile-TLS as a fast and high-resolution tool for measuring forest structural attributes and obtaining relevant ecological data.
Framework and Capability of Industrial IoT Infrastructure for Smart Manufacturing
Authors: Li Keng , Zhang Yu , Huang Yong , Tian Zhiwei , Sang Ziqin
Published: January 2023

The Internet of Things (IoT) and smart manufacturing (SM) are mutually reinforcing. The establishment of IoT-based common facilities for SM is the premise of building SM system. Industrial IoT (IIoT) infrastructure for SM refers to common facilities based on IoT that support SM in industries or sectors, and plays a dominant role and faces severe challenges in the intelligence of SM. The infrastructure is independent of the products and production process in a specific factory. This paper develops conceptual and capability frameworks of IIoT infrastructure from a unified perspective of IIoT-related SM industries. These frameworks reflect relationships between IIoT and SM with in-depth relationships among basic facilities of IIoT infrastructure and lay the foundation of SM. In this paper the common characteristics and high-level requirements with respect to the different IoT layers of IIoT infrastructure are analyzed, and the capability framework and relevant capabilities of IIoT infrastructure are summarized according to the characteristics and requirements. In order to help service providers implement their systems to meet the needs of SM, the existing and newly developed IIoT infrastructure are integrated partially or in whole according to the intelligence level, so as to provide technical guidance for stakeholders to apply emerging ICTs to SM.