bluuebunny/crossref_metadata_2025 huggingface dataset:
Household and Environmental Risk Factors for Kala-azar: A Case-Control Study in Tertiary Care Hospital of Bangladesh
Authors: Ahammed Afsar , Yusuf Md. Abdullah , Feroz Salahuddin , Selim Shahjada , Bhattacharyya Biswajit , Ahammed Imran , Rahman Md Ridwanur
Published: August 2017

Background: Visceral Leishmaniasis (VL), commonly known as Kala-azar is a chronic febrile disease occurs widely throughout the world. There are many risk factors which can influence the causation of kala azar in Bangladeshi people.Objective: The aim of the present study is to describe the Sociodemographic, household and environmental risk factors of kala-azar among a case series of Bangladeshi patients. Method: This case control study was carried out at inpatient department of Community Based Medical College Hospital, Mymensingh from July 2010 to June 2011, for a period of 1(one) year.  The study samples were clinically and parasitologically confirmed kala-azar cases. The controls were rK 39 strip test negative hospitalized cases admitted for other reason.Results: Univariate analysis showed that nature of wall in the main structure, floor of the house, presence of cracks and crevices in walls, presence of a granary inside houses and presence of bamboo trees near houses, were risk factors for kala-azar. Multivariate analysis showed that Presence of cracks and crevices in walls (OR=3.429, 95%CI=1.037-11.338, P =0.043) and presence of bamboo tree around houses (OR=5.652, 95%CI =1.368-23.347, P =0.017) in rural areas of study region, were significant risk factors for kala-azar.Conclusion: These findings have important practical implications because they suggest that on improving housing and environmental conditions  in rural areas, may be particularly effective in reducing the incidence of kala-azar and its transmission of infection by sand fly vectors.Journal of Science Foundation, 2016;14(2):56-61
Length of Operational Life and Its Impact on Life-Cycle Costs of a Tractor in Switzerland
Authors: Lips Markus
Published: August 2017

Assuming full exhaustion of their estimated service life—also referred to as estimated technical working life—the low annual utilisation of agricultural machines in Switzerland leads to a long operational life. To determine the costs of an additional year’s service, we calculate the life-cycle costs (LCCs), which encompass all costs for an 82 kW four-wheel-drive tractor over its entire operational life. Assuming full utilisation of the estimated service life of 10,000 h, five operating versions are compared, each with an operational life of between 10 and 30 years and matched annual utilisations between 1000 h and 333 h. A key finding is that an additional year in service increases LCCs by 4.5% of the tractor’s purchase price. In addition, we carry out a sensitivity analysis by applying discount rates of between 0% and 4%, finding that a comparatively high discount rate of 3% leads to almost identical LCCs for all operating versions. We conclude that the annual utilisation and the resulting duration of operational life have a strong impact on LCCs. A short operational life associated with high annual utilisation is a promising strategy for substantially cutting machinery costs.
Adoption of Web-Based Spatial Tools by Agricultural Producers: Conversations with Seven Northeastern Ontario Farmers Using the GeoVisage Decision Support System
Authors: Jarvis Daniel , Wachowiak Mark , Walters Dan , Kovacs John
Published: August 2017

This paper reports on the findings of a multi-site qualitative case study research project designed to document the utility and perceived usefulness of weather station and imagery data associated with the online resource GeoVisage among northeastern Ontario farmers. Interviews were conducted onsite at five participating farms (three dairy, one cash crop, and one public access fruit/vegetable) in 2014–2016, and these conversations were transcribed and returned to participants for member checking. Interview data was then entered into Atlas.ti software for the purpose of qualitative thematic analysis. Fifteen codes emerged from the data and findings center around three overarching themes: common uses of weather station data (e.g., air/soil temperature, rainfall); the use of GeoVisage Imagery data/tools (e.g., acreage calculations, remotely sensed imagery); and future recommendations for the online resource (e.g., communication, secure crop imagery, mobile access). Overall, weather station data and tools freely accessible through the GeoVisage site were viewed as representing a timely, positive, and important addition to contemporary agricultural decision-making in northeastern Ontario farming.
Chlorine Tolerance and Inactivation of Escherichia coli recovered from Wastewater Treatment Plants in the Eastern Cape, South Africa
Authors: Owoseni Mojisola , Olaniran Ademola , Okoh Anthony
Published: August 2017

This study investigated the survival of Escherichia coli (E. coli) recovered from secondary effluents of two wastewater treatment plants in the Eastern Cape Province, South Africa, in the presence of different chlorine concentrations. The bacterial survival, chlorine lethal dose and inactivation kinetics at lethal doses were examined. The bacterial isolates were identified by 16S rRNA gene sequencing. Comparison of the nucleotide sequences of 16S rRNA gene of bacteria with known taxa in the GenBank revealed the bacterial isolates to belong to Escherichia coli. At the recommended free chlorine of 0.5 mg/L, reduction of E. coli isolates (n = 20) initial bacterial concentration of 8.35–8.75 log was within a range of 3.88–6.0 log at chlorine residuals of 0.14–0.44 mg/L after 30 min. At higher doses, a marked reduction (p < 0.05) in the viability of E. coli isolates was achieved with a greater than 7.3 log inactivation of the bacterial population. Inactivation kinetics revealed a high rate of bacterial kill over time (R2 > 0.9) at chlorine dose of 1.5 mg/L. This study indicates poor removal of bacteria at free chlorine at 0.5 mg/L and a greater efficacy of 1.5 mg/L in checking E. coli tolerance.
Experimental Evaluation of Thermal Performance and Durability of Thermally-Enhanced Concretes
Authors: Jeong Yong-Wook , Koh Tae-Hoon , Youm Kwang-Soo , Moon Jiho
Published: August 2017

The thermal performance and durability of the thermally-enhanced concrete with various insulating materials were evaluated through a series of tests. Three types of insulating materials—diatomite powder, hollow micro-spheres, and a micro-foam agent—were used for both normalweight aggregate concrete (NWAC) and lightweight aggregate concrete (LWAC). The thermal conductivity was measured by two different test methods: quick thermal conductivity meter (QTM) and guarded hot wire (GHW) methods. Then, the results were compared with each other. All insulating materials used in this study proved their ability to reduce the thermal conductivity. Additionally, it can be found that the trend of a decrease in air-dry density is similar to that of thermal conductivity of thermally-enhanced concrete. Additional thermal transmission tests with seven large-scale specimens were conducted by using the calibrated hot box (CHB). However, from this tests, it was seen that thermal transmission reduction for tested specimens were not large compared to the thermal conductivity reduction measured by QTM and GHW, due to multiple heat transfer. To examine the durability of thermally-enhanced concretes, accelerated carbonation and freeze-thaw cycle tests were conducted. From the results, it can be found that the thermally-enhanced concrete shows good freeze-thaw resistance. However, the carbonation rates of the concretes increased rapidly and additional methods to improve the carbonation resistance should be considered.
Biosynthesis of Polyhydroxyalkanoate from Steamed Soybean Wastewater by a Recombinant Strain of Pseudomonas sp. 61-3
Authors: Hokamura Ayaka , Yunoue Yuko , Goto Saki , Matsusaki Hiromi
Published: August 2017

Pseudomonas sp. 61-3 accumulates a blend of poly(3-hydroxybutyrate) [P(3HB)] homopolymer and a random copolymer, poly(3-hydroxybutyrate-co-3-hydroxyalkanoate) [P(3HB-co-3HA)], consisting of 3HA units of 4–12 carbon atoms. Pseudomonas sp. 61-3 possesses two types of PHA synthases, PHB synthase (PhbC) and PHA synthases (PhaC1 and PhaC2), encoded by the phb and pha loci, respectively. The P(94 mol% 3HB-co-6 mol% 3HA) copolymer synthesized by the recombinant strain of Pseudomonas sp. 61-3 (phbC::tet) harboring additional copies of phaC1 gene is known to have desirable physical properties and to be a flexible material with moderate toughness, similar to low-density polyethylene. In this study, we focused on the production of the P(3HB-co-3HA) copolymer using steamed soybean wastewater, a by-product in brewing miso, which is a traditional Japanese seasoning. The steamed soybean wastewater was spray-dried to produce a powder (SWP) and used as the sole nitrogen source for the synthesis of P(3HB-co-3HA) by the Pseudomonas sp. 61-3 recombinant strain. Hydrolyzed SWP (HSWP) was also used as a carbon and nitrogen source. P(3HB-co-3HA)s with relatively high 3HB fractions could be synthesized by a recombinant strain of Pseudomonas sp. 61-3 (phbC::tet) harboring additional copies of the phaC1 gene in the presence of 2% glucose and 10–20 g/L SWP as the sole nitrogen source, producing a PHA concentration of 1.0–1.4 g/L. When HSWP was added to a nitrogen- and carbon-free medium, the recombinant strain could synthesize PHA without glucose as a carbon source. The recombinant strain accumulated 32 wt% P(3HB-co-3HA) containing 80 mol% 3HB and 20 mol% medium-chain-length 3HA with a PHA concentration of 1.0 g/L when 50 g/L of HSWP was used. The PHA production yield was estimated as 20 mg-PHA/g-HSWP, which equates to approximately 1.0 g-PHA per liter of soybean wastewater.
Protein-Mediated Biotemplating on the Nanoscale
Authors: Freeman Amihay
Published: August 2017

Purified proteins offer a homogeneous population of biological nanoparticles, equipped in many cases with specific binding sites enabling the directed self-assembly of envisaged one-, two- or three-dimensional arrays. These arrays may serve as nanoscale biotemplates for the preparation of novel functional composite materials, which exhibit potential applications, especially in the fields of nanoelectronics and optical devices. This review provides an overview of the field of protein-mediated biotemplating, focussing on achievements made throughout the past decade. It is comprised of seven sections designed according to the size and configuration of the protein-made biotemplate. Each section describes the design and size of the biotemplate, the resulting hybrid structures, the fabrication methodology, the analytical tools employed for the structural analysis of the hybrids obtained, and, finally, their claimed/intended applications and a feasibility demonstration (whenever available). In conclusion, a short assessment of the overall status of the achievements already made vs. the future challenges of this field is provided.
Mental Illness in Children: Childhood Illness and Supporting the Family
Authors: Sheehan Rosemary
Published: August 2017

Childhood is a stage of life that is filled with potential for development, and the early years of childhood see immense physical changes in growth; mastery over body functions like movement; the acquisition of language and cognitive development to understand their own and others’ thinking and reasoning; and the psychosocial development of trust in the world, comfort in the care they receive from parents and caregivers, and the sense of being secure in themselves that this engenders. [...]
Assessment of Sleep in Children with Autism Spectrum Disorder
Authors: Moore Makeda , Evans Victoria , Hanvey Grace , Johnson Cynthia
Published: August 2017

Sleep disturbances in children with autism spectrum disorder (ASD) are significantly more prevalent than found in typically developing (TD) children. Given the detrimental impact of poor sleep on cognitive, emotional, and behavioral functioning, it is imperative to screen and assess for sleep disturbances in this population. In this paper, we describe the screening and assessment process, as well as specific measures commonly used for assessing sleep in children with ASD. Advantages and limitations for use in children with ASD are discussed. While subjective measures, such as parent-report questionnaires and sleep diaries, are the most widely used, more objective measures such as actigraphy, polysomnography, and videosomnography provide additional valuable information for both diagnostic purposes and treatment planning. These objective measures, nonetheless, are limited by cost, availability, and feasibility of use with children with ASD. The current review provides an argument for the complementary uses of both subjective and objective measures of sleep specifically for use in children with ASD.
Multiscale Information Decomposition: Exact Computation for Multivariate Gaussian Processes
Authors: Faes Luca , Marinazzo Daniele , Stramaglia Sebastiano
Published: August 2017

Exploiting the theory of state space models, we derive the exact expressions of the information transfer, as well as redundant and synergistic transfer, for coupled Gaussian processes observed at multiple temporal scales. All of the terms, constituting the frameworks known as interaction information decomposition and partial information decomposition, can thus be analytically obtained for different time scales from the parameters of the VAR model that fits the processes. We report the application of the proposed methodology firstly to benchmark Gaussian systems, showing that this class of systems may generate patterns of information decomposition characterized by prevalently redundant or synergistic information transfer persisting across multiple time scales or even by the alternating prevalence of redundant and synergistic source interaction depending on the time scale. Then, we apply our method to an important topic in neuroscience, i.e., the detection of causal interactions in human epilepsy networks, for which we show the relevance of partial information decomposition to the detection of multiscale information transfer spreading from the seizure onset zone.
A Back-EMF Estimation Error Compensation Method for Accurate Rotor Position Estimation of Surface Mounted Permanent Magnet Synchronous Motors
Authors: Jung Tae-Uk , Jang Jung-Hoon , Park Chang-Seok
Published: August 2017

This paper proposes a back electromotive force estimation error compensation method for accurate rotor position estimation of surface mounted permanent magnet synchronous motors. When estimating the rotor position of surface mounted permanent magnet synchronous motor sensorless drives, a direct current offset error component occurs in the voltage sensor. As a result, the rotor position is distorted and the sensorless control in surface mounted permanent magnet synchronous motor is degraded. In addition, the dq-axis voltages in the synchronous reference frame have the direct current offset error component, ripples compared with the motor frequency under the distorted rotor position. In this paper, the effects of the direct current offset errors are analyzed based on the synchronous reference frame phase locked loop. To remove this direct current offset error component, a d-axis voltage is converted into a synchronous reference frame again to compensate. In other words, it is a dual synchronous coordinate conversion compensation method. The compensator utilizes a proportional-integral controller that compensates by estimating the direct current offset error component. The proposed method is useful for the improvement of surface mounted permanent magnet synchronous motor sensorless control and operating performance. The effectiveness of the proposed algorithm is verified through PSIM simulation and experimental results.
An Economical Evaluation of Anaerobic Digestion Plants Fed with Organic Agro-Industrial Waste
Authors: Carlini Maurizio , Mosconi Enrico , Castellucci Sonia , Villarini Mauro , Colantoni Andrea
Published: August 2017

Anaerobic co-digestion technology is increasingly used to simultaneously treat solid and liquid organic waste to balance nutrient content, to reduce the negative effects of toxic compounds in the process, and to increase biogas yield. The aim of this study was to analyze the economic performance of co-digestion plants fed with agro-industrial wastes as a function of installed power, with a method of discounted cash flow. The study focused on Italian framework conditions concerning payment for electricity produced by anaerobic digestion (AD) plants as well as the production costs. The economic analysis was carried out using three different plant sizes: 100 kW, 500 kW, and 1000 kW, which are representative of biogas plants in Italy. The study showed that the improvement of the break-point is closely linked to the increase in corresponding plant size. Given the assumptions of the simulation, the payback for the 100 kW plant was nine years for both the all-inclusive tariff and the basic feed-in tariff (BFT). Regarding the 500 kW and 1000 kW plants, the payback periods were five and four years, respectively.
Estimation of Forest Biomass Patterns across Northeast China Based on Allometric Scale Relationship
Authors: Ni Xiliang , Cao Chunxiang , Zhou Yuke , Ding Lin , Choi Sungho , Shi Yuli , Park Taejin , Fu Xiao , Hu Hong , Wang Xuejun
Published: August 2017

This study develops a modeling framework for utilizing the large footprint LiDAR waveform data from the Geoscience Laser Altimeter System (GLAS) onboard NASA’s Ice, Cloud, and Land Elevation Satellite (ICESat), Moderate Resolution Imaging Spectro-Radiometer (MODIS) imagery, meteorological data, and forest measurements for monitoring stocks of total biomass (including aboveground biomass and root biomass). The forest tree height models were separately used according to the artificial neural network (ANN) and the allometric scaling and resource limitation (ASRL) tree height models which can both combine the climate data and satellite data to predict forest tree heights. Based on the allometric approach, the forest aboveground biomass model was developed from the field measured aboveground biomass data and the tree heights derived from two tree height models. Then, the root biomass should scale with the aboveground biomass. To investigate whether this approach is efficient for estimating forest total biomass, we used Northeast China as the object of study. Our results generally proved that the method proposed in this study could be meaningful for forest total biomass estimation (R2 = 0.699, RMSE = 55.86).
A Note on Disbelief in Others regarding Backward Induction
Authors: Tutić Andreas , Grehl Sascha
Published: August 2017

We present experimental results on the role of beliefs in the cognitive ability of others in a problem involving backward induction. Using a modified version of the so-called race game, our design allows the effects of a player’s own inability to perform backward induction to be separated from the effects of her disbelief in the ability of others to do so. We find that behavior is responsive to the dependence on others who might fail in backward induction as well as information regarding their backward induction skills.
Hydrogel Microparticles as Sensors for Specific Adhesion: Case Studies on Antibody Detection and Soil Release Polymers
Authors: Strzelczyk Alexander Klaus , Wang Hanqing , Lindhorst Andreas , Waschke Johannes , Pompe Tilo , Kropf Christian , Luneau Benoit , Schmidt Stephan
Published: August 2017

Adhesive processes in aqueous media play a crucial role in nature and are important for many technological processes. However, direct quantification of adhesion still requires expensive instrumentation while their sample throughput is rather small. Here we present a fast, and easily applicable method on quantifying adhesion energy in water based on interferometric measurement of polymer microgel contact areas with functionalized glass slides and evaluation via the Johnson–Kendall–Roberts (JKR) model. The advantage of the method is that the microgel matrix can be easily adapted to reconstruct various biological or technological adhesion processes. Here we study the suitability of the new adhesion method with two relevant examples: (1) antibody detection and (2) soil release polymers. The measurement of adhesion energy provides direct insights on the presence of antibodies showing that the method can be generally used for biomolecule detection. As a relevant example of adhesion in technology, the antiadhesive properties of soil release polymers used in today’s laundry products are investigated. Here the measurement of adhesion energy provides direct insights into the relation between polymer composition and soil release activity. Overall, the work shows that polymer hydrogel particles can be used as versatile adhesion sensors to investigate a broad range of adhesion processes in aqueous media.
Ultraviolet Imaging of Volcanic Plumes: A New Paradigm in Volcanology
Authors: McGonigle Andrew J. S. , Pering Tom D. , Wilkes Thomas C. , Tamburello Giancarlo , D’Aleo Roberto , Bitetto Marcello , Aiuppa Alessandro , Willmott Jon R.
Published: August 2017

Ultraviolet imaging has been applied in volcanology over the last ten years or so. This provides considerably higher temporal and spatial resolution volcanic gas emission rate data than available previously, enabling the volcanology community to investigate a range of far faster plume degassing processes than achievable hitherto. To date, this has covered rapid oscillations in passive degassing through conduits and lava lakes, as well as puffing and explosions, facilitating exciting connections to be made for the first time between previously rather separate sub-disciplines of volcanology. Firstly, there has been corroboration between geophysical and degassing datasets at ≈1 Hz, expediting more holistic investigations of volcanic source-process behaviour. Secondly, there has been the combination of surface observations of gas release with fluid dynamic models (numerical, mathematical, and laboratory) for gas flow in conduits, in attempts to link subterranean driving flow processes to surface activity types. There has also been considerable research and development concerning the technique itself, covering error analysis and most recently the adaptation of smartphone sensors for this application, to deliver gas fluxes at a significantly lower instrumental price point than possible previously. At this decadal juncture in the application of UV imaging in volcanology, this article provides an overview of what has been achieved to date as well as a forward look to possible future research directions.
Microbial Effects on the Production of Aquaponically Grown Lettuce
Authors: Wielgosz Zachary , Anderson Tyler , Timmons Michael
Published: August 2017

In the study of aquaponics, questions have risen concerning the role of microbiota upon plant growth. This experiment compared unaltered aquaponic nutrient solution at pH 7.0 (treatment A7) with sterilized aquaponic nutrient solution at pH 7.0 (treatment SA7) to quantify effects that live microorganisms had on the growth of lettuce (Lactuca sativa). Baseline comparisons were made to conventional hydroponic conditions using inorganic nutrients at pH 5.8 (treatment H5) or pH 7.0 (treatment H7), which showed a 51% reduction in lettuce fresh weight (FW) growth, indicating a strong negative effect of the higher pH nutrient solution; a similar large difference (38%) occurred for dry weight (DW) comparisons between these two treatments. No difference occurred between the sterilized aquaponic treatment (SA7) and H5 in FW or DW. H5 produced more FW and DW than the non-sterile aquaponic treatment (A7). Responses were not different in FW and DW between the two aquaponic conditions. Reduced FW and DW of A7 compared to H5 may be related to the 50 times higher microbial community living in the A7 treatment water than in H5 at the beginning of each trial. Given that the sterile aquaponic solution SA7 and the H5 hydroponic condition were not different in performance, we concluded that the beneficial effects of the microbial community in the fish recirculating aquaculture system (RAS) occurred before the water was sterilized. An interesting implication of our findings is that an RAS can be decoupled from the hydroponic growing system, since its benefits are not dependent upon continuous recirculation between the plant and fish system and its living biotic community.
Adversity in University: Cyberbullying and Its Impacts on Students, Faculty and Administrators
Authors: Cassidy Wanda , Faucher Chantal , Jackson Margaret
Published: August 2017

This paper offers a qualitative thematic analysis of the impacts of cyberbullying on post-secondary students, faculty, and administrators from four participating Canadian universities. These findings were drawn from data obtained from online surveys of students and faculty, student focus groups, and semi-structured interviews with faculty members and university administrators. The key themes discussed include: negative affect, impacts on mental and physical health, perceptions of self, impacts regarding one’s personal and professional lives, concern for one’s safety, and the impact of authorities’ (non) response. Students reported primarily being cyberbullied by other students, while faculty were cyberbullied by both students and colleagues. Although students and faculty represent different age levels and statuses at the university, both groups reported similar impacts and similar frustrations at finding solutions, especially when their situations were reported to authorities. It is important that universities pay greater attention to developing effective research-based cyberbullying policies and to work towards fostering a more respectful online campus culture.
Estimated Maternal Pesticide Exposure from Drinking Water and Heart Defects in Offspring
Authors: Kim Jihye , Swartz Michael , Langlois Peter , Romitti Paul , Weyer Peter , Mitchell Laura , Luben Thomas , Ramakrishnan Anushuya , Malik Sadia , Lupo Philip , Feldkamp Marcia , Meyer Robert , Winston Jennifer , Reefhuis Jennita , Blossom Sarah , Bell Erin , Agopian A. ,   The National Birth Defects Prevention Study
Published: August 2017

Our objective was to examine the relationship between estimated maternal exposure to pesticides in public drinking water and the risk of congenital heart defects (CHD). We used mixed-effects logistic regression to analyze data from 18,291 nonsyndromic cases with heart defects from the Texas Birth Defects Registry and 4414 randomly-selected controls delivered in Texas from 1999 through 2005. Water district-level pesticide exposure was estimated by linking each maternal residential address to the corresponding public water supply district’s measured atrazine levels. We repeated analyses among independent subjects from the National Birth Defects Prevention Study (NBDPS) (1620 nonsyndromic cases with heart defects and 1335 controls delivered from 1999 through 2005). No positive associations were observed between high versus low atrazine level and eight CHD subtypes or all included heart defects combined. These findings should be interpreted with caution, in light of potential misclassification and relatively large proportions of subjects with missing atrazine data. Thus, more consistent and complete monitoring and reporting of drinking water contaminants will aid in better understanding the relationships between pesticide water contaminants and birth defects.
Diurnal Temperature Range in Relation to Daily Mortality and Years of Life Lost in Wuhan, China
Authors: Zhang Yunquan , Yu Chuanhua , Yang Jin , Zhang Lan , Cui Fangfang
Published: August 2017

Diurnal temperature range (DTR) is an important meteorological indicator associated with global climate change, and has been linked with mortality and morbidity in previous studies. To date, however, little evidence has been available regarding the association of DTR with years of life lost (YLL). This study aimed to evaluate the DTR-related burden on both YLL and mortality. We collected individual records of all registered deaths and daily meteorological data in Wuhan, central China, between 2009 and 2012. For the whole population, every 1 °C increase in DTR at a lag of 0–1 days was associated with an increase of 0.65% (95% CI: 0.08–1.23) and 1.42 years (−0.88–3.72) for mortality and YLL due to non-accidental deaths, respectively. Relatively stronger DTR-mortality/YLL associations were found for cardiovascular deaths. Subgroup analyses (stratified by gender, age, and education level) showed that females, the elderly (75+ years old), and those with higher education attainment (7+ years) suffered more significantly from both increased YLL and mortality due to large DTR. Our study added additional evidence that short-term exposure to large DTR was associated with increased burden of premature death using both mortality incidence and YLL.
Nationwide Point Cloud—The Future Topographic Core Data
Authors: Virtanen Juho-Pekka , Kukko Antero , Kaartinen Harri , Jaakkola Anttoni , Turppa Tuomas , Hyyppä Hannu , Hyyppä Juha
Published: August 2017

Topographic databases maintained by national mapping agencies are currently the most common nationwide data sets in geo-information. The application of laser scanning as source data for surveying is increasing. Along with this development, several analysis methods that utilize dense point clouds have been introduced. We present the concept of producing a dense nationwide point cloud, produced from multiple sensors and containing multispectral information, as the national core data for geo-information. Geo-information products, such as digital terrain and elevation models and 3D building models, are produced automatically from these data. We outline the data acquisition, processing, and application of the point cloud. As a national data set, a dense multispectral point cloud could produce significant cost savings via improved automation in mapping and a reduction of overlapping surveying efforts.
Wicked Water Points: The Quest for an Error Free National Water Point Database
Authors: Verplanke Jeroen , Georgiadou Yola
Published: August 2017

The Water Sector Development Programme (WSDP) of Tanzania aims to improve the performance of the water sector in general and rural water supply (RWS) in particular. During the first phase of the WSDP (2007 to 2014), implementing agencies developed information systems for attaining management efficiencies. One of these systems, the Water Point Mapping System (WPMS), has now been completed, and the database is openly available to the public, as part of the country’s commitment to the Open Government Partnership (OGP) initiative. The Tanzanian WPMS project was the first attempt to map “wall-to-wall” all rural public water points in an African nation. The complexity of the endeavor led to suboptimal results in the quality of the WPMS database, the baseline of the WPMS. The WPMS database was a means for the future monitoring of all rural water points, but its construction has become an end in itself. We trace the challenges of water point mapping in Tanzania and describe how the WPMS database was initially populated and to what effect. The paper conceptualizes errors found in the WPMS database as material, observational, conceptual and discursive, and characterizes them in terms of type, suspected origin and mitigation options. The discussion focuses on the consequences of open data scrutiny for the integrity of the WPMS database and the implications for monitoring wicked water point data.
The Effect of Reduced Graphene Oxide-Coated Biphasic Calcium Phosphate Bone Graft Material on Osteogenesis
Authors: Kim Jeong-Woo , Shin Yong , Lee Jin-Ju , Bae Eun-Bin , Jeon Young-Chan , Jeong Chang-Mo , Yun Mi-Jung , Lee So-Hyoun , Han Dong-Wook , Huh Jung-Bo
Published: August 2017

This study was conducted to evaluate the effect of biphasic calcium phosphate (BCP) coated with reduced graphene oxide (rGO) as bone graft materials on bone regeneration. The rGO-coated BCP bone graft material was fabricatied by mixing rGO and BCP at various concentrations. The surface charge of rGO-coated BCP was measured to be −14.43 mV, which formed a static electrostatic interaction. Cell viabilities were significantly diminished at higher concentrations of ≥100 μg/mL. The calvarial defects of 48 rats were implanted rGO-coated BCPs at a weight ratio of 2:1000 (rGO2), 4:1000 (rGO4), and 10:1000 (rGO10), repectively. BCP was used as a control group. The micro-CT and histological analysis were performed to evaluate new bone formation at 2 and 8 weeks after surgery. The results showed that the new bone volume (mm3) was significantly higher in the experimental groups than in the control group. Histological analysis showed that new bone areas (%) were significantly higher in the rGO2 and rGO10 than in the control, and significantly higher in rGO4 than in the rGO2 and rGO10. Conclusively, the rGO-coated BCP was found to be effective on osteogenesis and the concentration of the composite was an important factor.
Super-Resolution Localization Microscopy of γ-H2AX and Heterochromatin after Folate Deficiency
Authors: Bach Margund , Savini Claudia , Krufczik Matthias , Cremer Christoph , Rösl Frank , Hausmann Michael
Published: August 2017

Folate is an essential water-soluble vitamin in food and nutrition supplements. As a one-carbon source, it is involved in many central regulatory processes, such as DNA, RNA, and protein methylation as well as DNA synthesis and repair. Deficiency in folate is considered to be associated with an increased incidence of several malignancies, including cervical cancer that is etiologically linked to an infection with “high-risk” human papilloma viruses (HPV). However, it is still not known how a recommended increase in dietary folate after its deprivation affects the physiological status of cells. To study the impact of folate depletion and its subsequent reconstitution in single cells, we used quantitative chromatin conformation measurements obtained by super-resolution fluorescence microscopy, i.e., single molecule localization microscopy (SMLM). As a read-out, we examined the levels and the (re)positioning of γ-H2AX tags and histone H3K9me3 heterochromatin tags after immunostaining in three-dimensional (3D)-conserved cell nuclei. As model, we used HPV16 positive immortalized human keratinocytes that were cultivated under normal, folate deficient, and reconstituted conditions for different periods of time. The results were compared to cells continuously cultivated in standard folate medium. After 13 weeks in low folate, an increase in the phosphorylation of the histone H2AX was noted, indicative of an accumulation of DNA double strand breaks. DNA repair activity represented by the formation of those γ-H2AX clusters was maintained during the following 15 weeks of examination. However, the clustered arrangements of tags appeared to relax in a time-dependent manner. Parallel to the repair activity, the chromatin methylation activity increased as detected by H3K9me3 tags. The progress of DNA double strand repair was accompanied by a reduction of the detected nucleosome density around the γ-H2AX clusters, suggesting a shift from hetero- to euchromatin to allow access to the repair machinery. In conclusion, these data demonstrated a folate-dependent repair activity and chromatin re-organization on the SMLM nanoscale level. This offers new opportunities to further investigate folate-induced chromatin re-organization and the associated mechanisms.
Spatial Distribution Patterns of Root-Associated Bacterial Communities Mediated by Root Exudates in Different Aged Ratooning Tea Monoculture Systems
Authors: Arafat Yasir , Wei Xiaoya , Jiang Yuhang , Chen Ting , Saqib Hafiz , Lin Sheng , Lin Wenxiong
Published: August 2017

Positive plant–soil feedback depends on beneficial interactions between roots and microbes for nutrient acquisition; growth promotion; and disease suppression. Recent pyrosequencing approaches have provided insight into the rhizosphere bacterial communities in various cropping systems. However; there is a scarcity of information about the influence of root exudates on the composition of root-associated bacterial communities in ratooning tea monocropping systems of different ages. In Southeastern China; tea cropping systems provide the unique natural experimental environment to compare the distribution of bacterial communities in different rhizo-compartments. High performance liquid chromatography–electrospray ionization–mass spectrometry (HPLC–ESI–MS) was performed to identify and quantify the allelochemicals in root exudates. A high-throughput sequence was used to determine the structural dynamics of the root-associated bacterial communities. Although soil physiochemical properties showed no significant differences in nutrients; long-term tea cultivation resulted in the accumulation of catechin-containing compounds in the rhizosphere and a lowering of pH. Moreover; distinct distribution patterns of bacterial taxa were observed in all three rhizo-compartments of two-year and 30-year monoculture tea; mediated strongly by soil pH and catechin-containing compounds. These results will help to explore the reasons why soil quality and fertility are disturbed in continuous ratooning tea monocropping systems; and to clarify the associated problems.
Manipulation of the Growth Hormone-Insulin-Like Growth Factor (GH-IGF) Axis: A Treatment Strategy to Reverse the Effects of Early Life Developmental Programming
Authors: Reynolds Clare M. , Perry Jo K. , Vickers Mark H.
Published: August 2017

Evidence from human clinical, epidemiological, and experimental animal models has clearly highlighted a link between the early life environment and an increased risk for a range of cardiometabolic disorders in later life. In particular, altered maternal nutrition, including both undernutrition and overnutrition, spanning exposure windows that cover the period from preconception through to early infancy, clearly highlight an increased risk for a range of disorders in offspring in later life. This process, preferentially termed “developmental programming” as part of the developmental origins of health and disease (DOHaD) framework, leads to phenotypic outcomes in offspring that closely resemble those of individuals with untreated growth hormone (GH) deficiency, including increased adiposity and cardiovascular disorders. As such, the use of GH as a potential intervention strategy to mitigate the effects of developmental malprogramming has received some attention in the DOHaD field. In particular, experimental animal models have shown that early GH treatment in the setting of poor maternal nutrition can partially rescue the programmed phenotype, albeit in a sex-specific manner. Although the mechanisms remain poorly defined, they include changes to endothelial function, an altered inflammasome, changes in adipogenesis and cardiovascular function, neuroendocrine effects, and changes in the epigenetic regulation of gene expression. Similarly, GH treatment to adult offspring, where an adverse metabolic phenotype is already manifest, has shown efficacy in reversing some of the metabolic disorders arising from a poor early life environment. Components of the GH-insulin-like growth factor (IGF)-IGF binding protein (GH-IGF-IGFBP) system, including insulin-like growth factor 1 (IGF-1), have also shown promise in ameliorating programmed metabolic disorders, potentially acting via epigenetic processes including changes in miRNA profiles and altered DNA methylation. However, as with the use of GH in the clinical setting of short stature and GH-deficiency, the benefits of treatment are also, in some cases, associated with potential unwanted side effects that need to be taken into account before effective translation as an intervention modality in the DOHaD context can be undertaken.
Methylmercury Uptake into BeWo Cells Depends on LAT2-4F2hc, a System L Amino Acid Transporter
Authors: Balthasar Christina , Stangl Herbert , Widhalm Raimund , Granitzer Sebastian , Hengstschläger Markus , Gundacker Claudia
Published: August 2017

The organic mercury compound methylmercury (MeHg) is able to target the fetal brain. However, the uptake of the toxicant into placental cells is incompletely understood. MeHg strongly binds to thiol-S containing molecules such as cysteine. This MeHg-l-cysteine exhibits some structural similarity to methionine. System L plays a crucial role in placental transport of essential amino acids such as leucine and methionine and thus has been assumed to also transport MeHg-l-cysteine across the placenta. The uptake of methylmercury and tritiated leucine and methionine into the choriocarcinoma cell line BeWo was examined using transwell assay and small interfering (si)RNA mediated gene knockdown. Upon the downregulation of large neutral amino acids transporter (LAT)2 and 4F2 cell-surface antigen heavy chain (4F2hc), respectively, the levels of [3H]leucine in BeWo cells are significantly reduced compared to controls treated with non-targeting siRNA (p < 0.05). The uptake of [3H]methionine was reduced upon LAT2 down-regulation as well as methylmercury uptake after 4F2hc silencing (p < 0.05, respectively). These findings suggest an important role of system L in the placental uptake of the metal. Comparing the cellular accumulation of mercury, leucine, and methionine, it can be assumed that (1) MeHg is transported through system L amino acid transporters and (2) system L is responsible for the uptake of amino acids and MeHg primarily at the apical membrane of the trophoblast. The findings together can explain why mercury in contrast to other heavy metals such as lead or cadmium is efficiently transported to fetal blood.
Ionic Liquids as Grease Base Liquids
Authors: Mozes Robert , Cooper Peter , Atkin Rob , Li Hua
Published: August 2017

The rheological characteristics of one mineral oil and two ionic liquid (IL) based lubricating greases were explored as a function of thickener concentration. The ILs used are 1-butyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide ([BMIM][TFSI]) and trihexyltetradecylphosphonium bis(trifluoromethylsulfonyl)imide ([P6,6,6,14][TFSI]), with polytetra-fluoroethylene (PTFE) particles used as thickeners. Greases with different base liquid concentrations (60–80 wt %) were investigated using small-amplitude oscillatory shear and viscous flow measurements, and contact angle measurements probed adhesion at base liquid–PTFE interfaces. Rheological properties are influenced by base liquid–PTFE adhesion and the chemical structure of the grease base liquids. With the addition of thickener, the greases generally have higher elasticity, strain resistance, and frequency independent properties. Viscometric rheological tests illustrate non-Newtonian shear-thinning behaviour for all greases. [BMIM][TFSI] based greases show the most elastic properties and strain resistance, as well as the highest initial and lowest final viscosities of the greases tested.
Hydrophobic Coatings by Thiol-Ene Click Functionalization of Silsesquioxanes with Tunable Architecture
Authors: Dirè Sandra , Bottone Davide , Callone Emanuela , Maniglio Devid , Génois Isabelle , Ribot François
Published: August 2017

The hydrolysis-condensation of trialkoxysilanes under strictly controlled conditions allows the production of silsesquioxanes (SSQs) with tunable size and architecture ranging from ladder to cage-like structures. These nano-objects can serve as building blocks for the preparation of hybrid organic/inorganic materials with selected properties. The SSQs growth can be tuned by simply controlling the reaction duration in the in situ water production route (ISWP), where the kinetics of the esterification reaction between carboxylic acids and alcohols rules out the extent of organosilane hydrolysis-condensation. Tunable SSQs with thiol functionalities (SH-NBBs) are suitable for further modification by exploiting the simple thiol-ene click reaction, thus allowing for modifying the wettability properties of derived coatings. In this paper, coatings were prepared from SH-NBBs with different architecture onto cotton fabrics and paper, and further functionalized with long alkyl chains by means of initiator-free UV-induced thiol-ene coupling with 1-decene (C10) and 1-tetradecene (C14). The coatings appeared to homogeneously cover the natural fibers and imparted a multi-scale roughness that was not affected by the click functionalization step. The two-step functionalization of cotton and paper warrants a stable highly hydrophobic character to the surface of natural materials that, in perspective, suggests a possible application in filtration devices for oil-water separation. Furthermore, the purification of SH-NBBs from ISWP by-products was possible during the coating process, and this step allowed for the fast, initiator-free, click-coupling of purified NBBs with C10 and C14 in solution with a nearly quantitative yield. Therefore, this approach is an alternative route to get sol-gel-derived, ladder-like, and cage-like SSQs functionalized with long alkyl chains.
Modeling Adhesive Anchors in a Discrete Element Framework
Authors: Marcon Marco , Vorel Jan , Ninčević Krešimir , Wan-Wendner Roman
Published: August 2017

In recent years, post-installed anchors are widely used to connect structural members and to fix appliances to load-bearing elements. A bonded anchor typically denotes a threaded bar placed into a borehole filled with adhesive mortar. The high complexity of the problem, owing to the multiple materials and failure mechanisms involved, requires a numerical support for the experimental investigation. A reliable model able to reproduce a system’s short-term behavior is needed before the development of a more complex framework for the subsequent investigation of the lifetime of fasteners subjected to various deterioration processes can commence. The focus of this contribution is the development and validation of such a model for bonded anchors under pure tension load. Compression, modulus, fracture and splitting tests are performed on standard concrete specimens. These serve for the calibration and validation of the concrete constitutive model. The behavior of the adhesive mortar layer is modeled with a stress-slip law, calibrated on a set of confined pull-out tests. The model validation is performed on tests with different configurations comparing load-displacement curves, crack patterns and concrete cone shapes. A model sensitivity analysis and the evaluation of the bond stress and slippage along the anchor complete the study.
Neutron Reflectometry for Studying Corrosion and Corrosion Inhibition
Authors: Wood Mary , Clarke Stuart
Published: August 2017

Neutron reflectometry is an extremely powerful technique to monitor chemical and morphological changes at interfaces at the angstrom-level. Its ability to characterise metal, oxide and organic layers simultaneously or separately and in situ makes it an excellent tool for fundamental studies of corrosion and particularly adsorbed corrosion inhibitors. However, apart from a small body of key studies, it has yet to be fully exploited in this area. We present here an outline of the experimental method with particular focus on its application to the study of corrosive systems. This is illustrated with recent examples from the literature addressing corrosion, inhibition and related phenomena.