bluuebunny/crossref_metadata_2025 huggingface dataset:
Combined Transcriptome and Lipidomic Analyses of Lipid Biosynthesis in Macadamia ternifolia Nuts
Authors: Shi Rui , Bai Haidong , Li Biao , Liu Can , Ying Zhiping , Xiong Zhi , Wang Wenlin
Published: December 2021

Macadamia nuts are considered a high-quality oil crop worldwide. To date, the lipid diversity and the genetic factors that mediate storage lipid biosynthesis in Macadamia ternifolia are poorly known. Here, we performed a comprehensive transcriptomic and lipidomic data analysis to understand the mechanism of lipid biosynthesis by using young, medium-aged, and mature fruit kernels. Our lipidomic analysis showed that the M. ternifolia kernel was a rich source of unsaturated fatty acids. Moreover, different species of triacylglycerols, diacylglycerol, ceramides, phosphatidylethanolamine, and phosphatidic acid had altered accumulations during the developmental stages. The transcriptome analysis revealed a large percentage of differently expressed genes during the different stages of macadamia growth. Most of the genes with significant differential expression performed functional activity of oxidoreductase and were enriched in the secondary metabolite pathway. The integration of lipidomic and transcriptomic data allowed for the identification of glycerol-3-phosphate acyltransferase, diacylglycerol kinase, phosphatidylinositols, nonspecific phospholipase C, pyruvate kinase 2, 3-ketoacyl-acyl carrier protein reductase, and linoleate 9S-lipoxygenase as putative candidate genes involved in lipid biosynthesis, storage, and oil quality. Our study found comprehensive datasets of lipidomic and transcriptomic changes in the developing kernel of M. ternifolia. In addition, the identification of candidate genes provides essential prerequisites to understand the molecular mechanism of lipid biosynthesis in the kernel of M. ternifolia.
Considering Predictive Factors in the Diagnosis of Clinically Significant Prostate Cancer in Patients with PI-RADS 3 Lesions
Authors: Natale Caleb , Koller Christopher R. , Greenberg Jacob W. , Pincus Joshua , Krane Louis S.
Published: December 2021

The use of multi-parametric magnetic resonance imaging (mpMRI) in conjunction with the Prostate Imaging Reporting and Data System (PI-RADS) is standard practice in the diagnosis, surveillance, and staging of prostate cancer. The risk associated with lesions graded at a PI-RADS score of 3 is ambiguous. Further characterization of the risk associated with PI-RADS 3 lesions would be useful in guiding further work-up and intervention. This study aims to better characterize the utility of PI-RADS 3 and associated risk factors in detecting clinically significant prostate cancer. From a prospectively maintained IRB-approved dataset of all veterans undergoing mpMRI fusion biopsy at the Southeastern Louisiana Veterans Healthcare System, we identified a cohort of 230 PI-RADS 3 lesions from a dataset of 283 consecutive UroNav-guided biopsies in 263 patients from October 2017 to July 2020. Clinically significant prostate cancer (Gleason Grade ≥ 2) was detected in 18 of the biopsied PI-RADS 3 lesions, representing 7.8% of the overall sample. Based on binomial analysis, PSA densities of 0.15 or greater were predictive of clinically significant disease, as was PSA. The location of the lesion within the prostate was not shown to be a statistically significant predictor of prostate cancer overall (p = 0.87), or of clinically significant disease (p = 0.16). The majority of PI-RADS 3 lesions do not represent clinically significant disease; therefore, it is possible to reduce morbidity through biopsy. PSA density is a potential adjunctive factor in deciding which patients with PI-RADS 3 lesions require biopsy. Furthermore, while the risk of prostate cancer for African-American men has been debated in the literature, our findings indicate that race is not predictive of identifying prostate cancer, with comparable Gleason grade distributions on histology between races.
Bilateral Optic Disc Swelling as a Plausible Common Ocular Sign of Autoinflammatory Diseases: Report of Three Patients with Blau Syndrome or Cryopyrin-Associated Periodic Syndrome
Authors: Matsuo Toshihiko , Yashiro Masato , Yamasaki Osamu , Tanaka Takehiro , Manki Akira
Published: December 2021

The aim of this study is to describe bilateral optic disc swelling in three consecutive patients with Blau syndrome or cryopyrin-associated periodic syndrome at a single institution. Case 1 was a 30-year-old woman receiving 25 mg etanercept twice weekly who had been diagnosed as early-onset sarcoidosis by biopsy of skin rashes at 5 months old and genetically diagnosed with Blau syndrome with CARD15/NOD2 mutation (N670K) at 13 years old. At 10 years old, she began to have uveitis with optic disc swelling in both eyes, resulting in macular degeneration and optic disc atrophy at 17 years old only when etanercept was introduced. Case 2 was a 21-year-old man receiving adalimumab every 2 weeks who had been diagnosed as early-onset sarcoidosis by biopsy of skin rashes at 1.5 years old and genetically diagnosed as Blau syndrome with CARD15/NOD2 mutation (C495Y) at 5 years old. At 8 years old, around the time of adalimumab introduction, he began to show bilateral optic disc swelling which continued until the age of 16 years when the dose of adalimumab was increased. Case 3 was a 20-year-old woman receiving canakinumab every 8 weeks for systemic symptoms such as fever, headache, vomiting, and abdominal pain and later for sensorineural hearing disturbance on both sides. She had been diagnosed genetically with cryopyrin-associated periodic syndrome with NLRP3 mutation (Y859C) at 7 years old. At 5 years old, she was found to have bilateral optic disc swelling, which continued until the age of 10 years when she began receiving canakinumab (IL-1β inhibitor). Bilateral optic disc swelling might be tentatively designated as a plausible common ocular feature, if it occurred, in autoinflammatory diseases to pay more attention to ophthalmic complications in rare diseases.
Dynamics of Spring Regrowth and Comparative Production Performance of 50 Autumn-Sown Alfalfa Cultivars in the Coastal Saline Soil of North China
Authors: Wang Shichao , Fang Dong , Ameen Asif , Li Xiaolin , Guo Kai , Liu Xiaojing , Han Lipu
Published: December 2021

Alfalfa (Medicago sativa L.) production is affected by many factors, including management practices, soil conditions, and the environmental elements of the target area. Varietal differences, in terms of agronomic performance and forage yield, among 50 alfalfa cultivars under six harvest systems following regrowth were evaluated during the growing season of 2019–2020 under non-irrigated rainfed conditions in a coastal saline-alkali soil region of North China. Days to harvesting, plant height, canopy area, growth rate, and forage yield were assessed to rank the cultivars. Furthermore, the key factor influencing the regrowth of the second year after over-wintering was identified based on the growth status before over-wintering by using the Boston Matrix method. Results showed significant (p < 0.05) differences among cultivars and harvests regarding plant height, canopy area, and forage yield. Alfalfa forage yield ranged between 24.2 t ha−1 yr−1 and 32.7 t ha−1 yr−1. The highest forage yield was obtained in cultivar Guochan No.1, and was lowest in cultivar Magnum 601. Forage yield reached the greatest values for the first harvest, and then decreased gradually and changed stably. The forage yield of the third, fourth, fifth, and sixth harvest ranged from 3.4 t ha−1 to 4.3 t ha−1 (averaged across 50 cultivars), which represented 10.8% to 15.2% of the annual total forage production. We also observed that forage yield correlated strongly, but negatively, with the growth rate. According to subordinate function value analysis, Womu No.1, WL440HQ, Weston, Surprise, and WL354HQ proved optimum cultivars for general cultivation in this coastal area. In future, development of alfalfa cultivars with improved regrowth and tolerance to heavy saline-alkali soil and early spring drought would be necessary to increase forage yield under rainfed conditions in coastal saline-alkali areas of North China.
Psychosocial Risk and Health Behaviors as Predictors of Clinical Events in Patients Wait-Listed for a New Heart: Results from 7 Years of Follow-Up
Authors: Gali Kathleen , Weidner Gerdi , Smits Jacqueline M. A. , Beyersmann Jan , Spaderna Heike
Published: December 2021

We examined the long-term relationship of psychosocial risk and health behaviors on clinical events in patients awaiting heart transplantation (HTx). Psychosocial characteristics (e.g., depression), health behaviors (e.g., dietary habits, smoking), medical factors (e.g., creatinine), and demographics (e.g., age, sex) were collected at the time of listing in 318 patients (82% male, mean age = 53 years) enrolled in the Waiting for a New Heart Study. Clinical events were death/delisting due to deterioration, high-urgency status transplantation (HU-HTx), elective transplantation, and delisting due to clinical improvement. Within 7 years of follow-up, 92 patients died or were delisted due to deterioration, 121 received HU-HTx, 43 received elective transplantation, and 39 were delisted due to improvement. Adjusting for demographic and medical characteristics, the results indicated that frequent consumption of healthy foods (i.e., foods high in unsaturated fats) and being physically active increased the likelihood of delisting due improvement, while smoking and depressive symptoms were related to death/delisting due to clinical deterioration while awaiting HTx. In conclusion, psychosocial and behavioral characteristics are clearly associated with clinical outcomes in this population. Interventions that target psychosocial risk, smoking, dietary habits, and physical activity may be beneficial for patients with advanced heart failure waiting for a cardiac transplant.
Russian First World War Propaganda Literature through Its Anthologies. Some Observations on Russian Soldier-Literature and Journalistic Reporting
Authors: Cortesi Luca
Published: December 2021

In the Soviet era, Russian involvement in WWI long represented an ostracised and even forgotten event. This very attitude is reflected by Soviet literary criticism of WWI war literature. Taking into account both the studies which re-examined this part of Russian literature in a less ideologically biased manner and the stances that major writers of that period took towards the war, the aim of this paper is to investigate Russian Soldier-literature as presented in anthologies published in the wake of the First World War. The publishing of short stories, journalistic reporting and poems actually (or allegedly) composed by soldiers themselves can be interpreted as a symptomatic expression of a broader cultural discourse that was common at that time, and of which state propaganda publications often availed themselves.
Effect of Coating and Low Viscosity Oils on Piston Ring Friction under Mixed Regime of Lubrication through Analytical Modelling
Authors: Zavos Anastasios
Published: December 2021

This paper presents the impact of coating topography in piston ring-liner conjunction under mixed regime of lubrication using low viscosity oils. The study provides a time efficient analytical model including mixed-hydrodynamics regime of lubrication under different contact conditions. The method modified the expressions of the contact load and area of Greenwood-Tripp model in order to capture the real asperities interaction into contact. The model represents the tribological behavior of a thin top ring at Top Dead Centre, where boundary and mixed conditions are predominant. Electroplated CrN and PVD TiN coated rings were studied to predict the ring friction. The results are compared with an uncoated steel ring. The CrN coating shows slighter coefficient of friction, due to the coating morphology and roughness parameters. The TiN coating presents thicker lubricant films and higher coefficient of friction because the surface topography is quite rough with high peaks. This can be explained because of the major contribution of the roughness parameter and asperity slope in the boundary friction prediction.
Compliance with Prescription Guidelines for Glucose-Lowering Therapies According to Renal Function: Real-Life Study in Inpatients of Internal Medicine, Endocrinology and Cardiology Units
Authors: Lohan Laura , Galtier Florence , Manson Thibault , Mura Thibault , Castet-Nicolas Audrey , Faure Delinger , Chapet Nicolas , Leclercq Florence , Pasquié Jean Luc , Roubille François , Roubille Camille , Blain Hubert , Guilpain Philippe , Villiet Maxime , Avignon Antoine , Sultan Ariane , Breuker Cyril
Published: December 2021

Background and objectives: Renal failure is a contraindication for some glucose-lowering drugs and requires dosage adjustment for others, particularly biguanides, sulfonylureas, and inhibitors of dipeptidyl peptidase 4. In this study, we assessed adherence to prescription recommendations for glucose-lowering drugs according to renal function in hospitalized diabetic subjects. Materials and Methods: This prospective cohort study was carried out over a 2-year period in a university hospital. Glomerular filtration rate (GFR) was determined by averaging all measurements performed during hospitalization. Glucose-lowering drug dosages were analyzed according to the recommendations of the relevant medical societies. Results: In total, 2071 diabetic patients (53% hospitalized in cardiology units) were examined. GFR was <30 mL/min/1.73 m2 in 13.4% of these patients, 30–44 in 15.1%, 45–60 in 18.3%, and >60 in 53.3%. Inappropriate oral glucose-lowering treatments were administered to 273 (13.2%) patients, including 53 (2.6%) with a contraindication. In cardiology units, 53.1% and 14.3% of patients had GFRs of <60 and <30 mL/min/1.73 m2, respectively, and 179 (15.4%) patients had a contraindication or were prescribed an excessive dose of glucose-lowering drugs. Conclusions: We showed that the burden of inappropriate prescriptions is high in diabetic patients. Given the high number of patients receiving these medications, particularly in cardiology units, a search for potential adverse effects related to these drugs should be performed.
The Dynamics of Inflammatory Markers in Patients with Suspected Acute Appendicitis
Authors: Kaminskas Ąžuolas Algimantas , Lukšaitė-Lukštė Raminta , Jasiūnas Eugenijus , Samuilis Artūras , Augustinavičius Vytautas , Kryžauskas Marius , Strupas Kęstutis , Poškus Tomas
Published: December 2021

Background: Laboratory tests of inflammatory mediators are routinely used in the diagnosis of acute appendicitis (AA). The aim of this study was to evaluate the differences of dynamics of inflammatory markers of the blood in patients with suspected acute appendicitis between complicated AA (CAA), non-complicated AA (NAA), and when AA was excluded (No-AA). Methods: This was a retrospective analysis of prospectively collected data of patients presented to the Emergency Department (ER) of a tertiary hospital center during a three-year period. All patients suspected of acute appendicitis were prospectively registered from 1 January 2016 to 31 December 2018. The dynamics of inflammatory markers of the blood between different types of AA (No-AA, NAA or CAA) during different periods of time are presented. Results: A total of 453 patients were included in the study, with 297 patients in the No-AA group, 99 in the NAA group, and 57 in the CAA group. White blood cell (WBC) count in the No-AA decreased with time, with a statistically significant difference between the <8 h and 25–72 h group. The neutrophils (NEU) percentage decreased in the No-AA group and was statistically significantly different between the <8 h and 25–72 h and <8 h and >72 h groups. C-reactive protein (CRP) increased significantly in the No-AA group throughout all time intervals, and from the first 24 h to the 25–72 h in the NAA and CAA groups. There was a statistically significant difference between the WBC count between No-AA, NAA, and No-AA and CAA groups during the first 24 and 24–48 h. There was a statistically significant difference between NEU percentage and LYMP percentage and in the NEU/LYMP ratio between No-AA and CAA groups through all time periods. CRP was significantly higher in the first 24 h in the CAA than in the No-AA group, and in the 24–48 h in the CAA group than in the No-AA and NAA groups. The linear logistic regression model, involving inflammatory mediators and clinical characteristics, showed mediocre diagnostic accuracy for diagnosing AA with an AUC of 0.737 (0.671–0.802). Conclusions: Increasing concentrations of inflammatory markers are more characteristic in CAA patients than in No-AA during the first 48 h after onset of the disease. A combination of laboratory tests with clinical signs and symptoms has a mediocre diagnostic accuracy in suspecting AA.
Surface Properties of Synaptosomes in the Presence of L-Glutamic and Kainic Acids: In Vitro Alteration of the ATPase and Acetylcholinesterase Activities
Authors: Doltchinkova Virjinia , Mouleshkova Nevena , Vitkova Victoria
Published: December 2021

Morphologically and functionally identical to brain synapses, the nerve ending particles synaptosomes are biochemically derived membrane structures responsible for the transmission of neural information. Their surface and mechanical properties, measured in vitro, provide useful information about the functional activity of synapses in the brain in vivo. Glutamate and kainic acid are of particular interest because of their role in brain pathology (including causing seizure, migraine, ischemic stroke, aneurysmal subarachnoid hemorrhage, intracerebral hematoma, traumatic brain injury and stroke). The effects of the excitatory neurotransmitter L-glutamic acid and its agonist kainic acid are tested on Na+, K+-ATPase and Mg2+-ATPase activities in synaptic membranes prepared from the cerebral cortex of rat brain tissue. The surface parameters of synaptosome preparations from the cerebral cortex in the presence of L-glutamic and kainic acids are studied by microelectrophoresis for the first time. The studied neurotransmitters promote a significant increase in the electrophoretic mobility and surface electrical charge of synaptosomes at 1–4 h after isolation. The measured decrease in the bending modulus of model bimolecular membranes composed of monounsaturated lipid 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine provides evidence for softer membranes in the presence of L-glutamate. Kainic acid does not affect membrane mechanical stability even at ten-fold higher concentrations. Both the L-glutamic and kainic acids reduce acetylcholinesterase activity and deviation from the normal functions of neurotransmission in synapses is presumed. The presented results regarding the modulation of the enzyme activity of synaptic membranes and surface properties of synaptosomes are expected by biochemical and biophysical studies to contribute to the elucidation of the molecular mechanisms of neurotransmitters/agonists’ action on membranes.
Effects of NH3 Plasma and Mg Doping on InGaZnO pH Sensing Membrane
Authors: Kao Chyuan-Haur , Liu Chia-Shao , Chan Shih-Ming , Kuo Chih-Chen , Tsai Shang-Che , Lee Ming-Ling , Chen Hsiang
Published: December 2021

In this study, the effects of magnesium (Mg) doping and Ammonia (NH3) plasma on the pH sensing capabilities of InGaZnO membranes were investigated. Undoped InGaZnO and Mg-doped pH sensing membranes with NH3 plasma were examined with multiple material analyses including X-ray diffraction, X-ray photoelectron spectroscopy, secondary ion mass spectroscopy and transmission electron microscope, and pH sensing behaviors of the membrane in electrolyte-insulator-semiconductors. Results indicate that Mg doping and NH3 plasma treatment could superpositionally enhance crystallization in fine nanostructures, and strengthen chemical bindings. Results indicate these material improvements increased pH sensing capability significantly. Plasma-treated Mg-doped InGaZnO pH sensing membranes show promise for future pH sensing biosensors.
Fabrication of Suspended PMMA-Graphene Membrane for High Sensitivity LC-MEMS Pressure Sensor
Authors: Yusof Norliana , Bais Badariah , Yunas Jumril , Soin Norhayati , Majlis Burhanuddin Yeop
Published: December 2021

The LC-MEMS pressure sensor is an attractive option for an implantable sensor. It senses pressure wirelessly through an LC resonator, eliminating the requirement for electrical wiring or a battery system. However, the sensitivity of LC-MEMS pressure sensors is still comparatively low, especially in biomedical applications, which require a highly-sensitive sensor to measure low-pressure variations. This study presents the microfabrication of an LC wireless MEMS pressure sensor that utilizes a PMMA-Graphene (PMMA/Gr) membrane supported on a silicon trench as the deformable structure. The (PMMA/Gr) membrane was employed to increase the sensor’s sensitivity due to its very low elastic modulus making it easy to deform under extremely low pressure. The overall size of the fabricated sensor was limited to 8 mm × 8 mm. The experimental results showed that the capacitance value changed from 1.64 pF to 12.32 pF when the applied pressure varied from 0 to 5 psi. This capacitance variation caused the frequency response to change from 28.74 MHz to 78.76 MHz. The sensor sensitivity was recorded with a value of 193.45 kHz/mmHg and a quality factor of 21. This study concludes that the (PMMA/Gr) membrane-based LC-MEMS pressure sensor has been successfully designed and fabricated and shows good potential in biomedical sensor applications.
Effect of Sigma Phase in Wire Arc Additive Manufacturing of Superduplex Stainless Steel
Authors: Akselsen Odd M. , Bjørge Ruben , Ånes Håkon Wiik , Ren Xiaobo , Nyhus Bård
Published: December 2021

In the present study, the thermal program in wire and arc additive manufacturing has been varied in terms of heat input and interpass temperature. Three walls were completed with subsequent Charpy V impact toughness and crack-tip opening displacement fracture toughness, together with a detailed microstructure characterization using light microscopy and scanning and transmission electron microscopy. The results clearly demonstrate that the formation of sigma phase may deteriorate the toughness of superduplex components. Such formation may take place under prolonged cooling time, which may occur when subsequent passes are deposited with too high interpass temperatures. This transformation behavior may limit the productivity in additive manufacturing of such steels and care must be taken in selection of proper combination of arc energy and interpass temperature.
Fracture Toughness Characteristics of High-Manganese Austenitic Steel Plate for Application in a Liquefied Natural Gas Carrier
Authors: An Gyubaek , Park Jeongung , Park Hongkyu , Han Ilwook
Published: December 2021

High-manganese austenitic steel was developed to improve the fracture toughness and safety of steel under cryogenic temperatures, and its austenite structure was formed by increasing the Mn content. The developed high-manganese austenitic steel was alloyed with austenite-stabilizing elements (e.g., C, Mn, and Ni) to increase cryogenic toughness. It was demonstrated that 30 mm thickness high-manganese austenitic steel, as well as joints welded with this steel, had a sufficiently higher fracture toughness than the required toughness values evaluated under the postulated stress conditions. High-manganese austenitic steel can be applied to large offshore and onshore LNG storage and fuel tanks located in areas experiencing cryogenic conditions. Generally, fracture toughness decreases at lower temperatures; therefore, cryogenic steel requires high fracture toughness to prevent unstable fractures. Brittle fracture initiation and arrest tests were performed using 30 mm thickness high-manganese austenitic steel and SAW joints. The ductile fracture resistance of the weld joints (weld metal, fusion line, fusion line + 2 mm) was investigated using the R-curve because a crack in the weld joint tends to deviate into the weld metal in the case of undermatched joints. The developed high-manganese austenitic steel showed little possibility of brittle fracture and a remarkably unstable ductile fracture toughness.
Inconel 713C Coating by Cold Spray for Surface Enhancement of Inconel 718
Authors: Wu Kaiqiang , Chee Sin Wei , Sun Wen , Tan Adrian Wei-Yee , Tan Sung Chyn , Liu Erjia , Zhou Wei
Published: December 2021

Inconel 713C is a nickel-based superalloy usually considered as a material of poor weldability due to its susceptibility to hot cracking in the heat-affected zones. Cold spray, a solid-state deposition technology that does not involve melting, can be proposed as a methodology to deposit Inconel 713C for surface enhancement of other target components. In this study, Inconel 713C coating was deposited on Inconel 718 substrate with a high-pressure cold spray system. The coating was characterized in terms of microstructure, hardness, and wear properties. The cold-sprayed Inconel 713C coating has a low porosity level and refined grain structures. Microhardness of the Inconel 713C coating was much higher than the Inconel 718 substrate. The sliding wear tests showed that the wear resistance of the cold-sprayed Inconel 713C coating is three times higher than the Inconel 718 substrate, making the coating a suitable protective layer. The main wear mechanisms of the coating include oxidation, tribo-film formation, and adhesive wear.
Effect of Ta and W Additions on Microstructure and Mechanical Properties of Tilt-Cast Ti-45Al-5Nb-2C Alloy
Authors: Lapin Juraj , Kamyshnykova Kateryna
Published: December 2021

The effect of Ta and W additions on microstructure and mechanical properties of tilt-cast Ti-45Al-5Nb-2C (at.%) alloy was investigated. Three alloys with nominal composition Ti-45Al-5Nb-2C-2X (in at.%), where X is Ta or W, were prepared by vacuum induction melting in graphite crucibles followed by tilt casting into graphite moulds. The microstructure of the tilt-cast alloys consists of the α2(Ti3Al) + γ(TiAl) lamellar grains, single γ phase, (Ti,Nb,X)2AlC particles with a small amount of (Ti,Nb,X)C, and β/B2 phase identified only in W containing alloy. The EDS analysis shows that Ta segregates into the carbide particles and reduces dissolution of Nb in both (Ti,Nb,Ta)C and (Ti,Nb,Ta)2AlC phases. The alloying with W reduces Nb content in both carbide phases and leads to stabilisation of β/B2 phase in the lamellar α2 + γ regions. The alloying with Ta and W does not affect the volume fraction of the carbide particles but influences their size and morphology. While the alloying with Ta and W has no significant effect on Vickers hardness and the indentation elastic modulus of the studied alloys, the addition of Ta affects the nanohardness and elastic modulus of the (Ti,Nb,Ta)2AlC phase. The addition of W significantly increases the Vickers microhardness of the lamellar α2 + γ regions.
Effect of Multi-Step Austempering Treatment on the Microstructure and Mechanical Properties of a High Silicon Carbide-Free Bainitic Steel with Bimodal Bainite Distribution
Authors: Franceschi Mattia , Miotti Bettanini Alvise , Pezzato Luca , Dabalà Manuele , Jacques Pascal J.
Published: December 2021

The effect of multi-step austempering treatments on the microstructure and mechanical properties of a novel medium carbon high silicon carbide-free bainitic steel was studied. Five different isothermal treatment processes were selected, including single-step isothermal treatments above martensite start temperature (at 350 °C and 370 °C, respectively), and three kinds of two-step routes (370 °C + 300 °C, 370 °C + 250 °C, and 350 °C + 250 °C). In comparison with single-step austempering treatment adopting a two-step process, a microstructure with a bimodal-size distribution of bainitic ferrite and without martensite was obtained. Bainitic transformation was studied using dilatometry both for single-step and two-step routes and the specimens were completely characterised by electron microscopy (SEM and TEM), X-ray diffraction (XRD) and standard tensile tests. The mechanical response of the samples subjected to two-step routes was superior to those treated at a single temperature.
MQL-Assisted Hard Turning of AISI D2 Steel with Corn Oil: Analysis of Surface Roughness, Tool Wear, and Manufacturing Costs
Authors: Arsene Bogdan , Gheorghe Catalin , Sarbu Flavius Aurelian , Barbu Magdalena , Cioca Lucian-Ionel , Calefariu Gavrila
Published: December 2021

Precision hard turning (HT) gained more and more attention in the cutting industry in the last years due to continuous pressure of the global market for reducing costs, minimizing the environmental and health issues, and achieving a cleaner production. Therefore, dry cutting and minimal quantity lubrication (MQL) became widely used in manufacturing to meet the environmental issues with respect to harmful cutting fluids (CFs). Vegetable oils, in MQL machining, are a promising solutions to petroleum-based CFs; however, the effects and performance on surface roughness and tool wear in HT with ceramic inserts remain unclear. To address this limitation, hardened AIDI D2 steel and pure corn oil, rich in saturated and monounsaturated fatty acids, cheap and widely available, have been used to conduct dry and MQL experiments at different cutting speed and feeds. Results show that corn oil is suitable as cutting lubricant in HT, creating a strong anti-wear and anti-friction lubricating film which improves the roughness with 10–15% and tool life with 15–20%, therefore reducing costs. Best surface roughness values (Ra = 0.151 μm, Rz = 0.887 μm, Rpk = 0.261 μm) were obtained at 180 m/min and 0.1 mm/rev. The analysis of variance shows that corn oil has statistical significance on roughness, validating the results.
Effect of the Length of Oat Hay on Growth Performance, Health Status, Behavior Parameters and Rumen Fermentation of Holstein Female Calves
Authors: Xiao Jianxin , Chen Tianyu , Alugongo Gibson Maswayi , Khan Muhammad Zahoor , Li Tingting , Ma Jing , Liu Shuai , Wang Wei , Wang Yajing , Li Shengli , Cao Zhijun
Published: December 2021

The aim of this study was to evaluate the effect of the length of oat hay on the performance, health, behavior, and rumen fermentation of dairy calves. For this purpose, two hundred and ten healthy two-day-old Holstein dairy calves were randomly allocated into three groups: basic diet (calf starter) without hay (CON), or a basic diet with oat hay at either long (OL: 10–12 cm) or short (OS: 3–5 cm) length cut. The basic diet was fed from day 4, while the hay was offered from day 14. All calves were weaned at day 56 and remained in their individual hutches till the end of the trial (day 70). Calf starter intake and fecal scores were recorded daily. Bodyweight, body size, and rumen fluid samples were collected biweekly before weaning and weekly after weaning. Overall, providing oat hay (OS and OL) in the diet increased the body weight, starter intake, and average daily gain compared to the CON group. Similarly, feeding oat hay improved rumen fermentation. More specifically, hay enhanced the rumen pH and changed the rumen fermentation type. Hay fed calves spent more time on rumination but less time performing abnormal behaviors compared to control. As it can be concluded, feeding oat hay to calves enhances the growth performance, rumen fermentation, and normal calf behaviors, implying improved animal welfare irrespective of the hay length.
1H-Nuclear Magnetic Resonance Analysis of Urine as Diagnostic Tool for Organic Acidemias and Aminoacidopathies
Authors: Pulido Ninna , Guevara-Morales Johana M. , Rodriguez-López Alexander , Pulido Álvaro , Díaz Jhon , Edrada-Ebel Ru Angelie , Echeverri-Peña Olga Y.
Published: December 2021

The utility of low-resolution 1H-NMR analysis for the identification of biomarkers provided evidence for rapid biochemical diagnoses of organic acidemia and aminoacidopathy. 1H-NMR, with a sensitivity expected for a field strength of 400 MHz at 64 scans was used to establish the metabolomic urine sample profiles of an infant population diagnosed with small molecule Inborn Errors of Metabolism (smIEM) compared to unaffected individuals. A qualitative differentiation of the 1H-NMR spectral profiles of urine samples obtained from individuals affected by different organic acidemias and aminoacidopathies was achieved in combination with GC–MS. The smIEM disorders investigated in this study included phenylalanine metabolism; isovaleric, propionic, 3-methylglutaconicm and glutaric type I acidemia; and deficiencies in medium chain acyl-coenzyme and holocarboxylase synthase. The observed metabolites were comparable and similar to those reported in the literature, as well as to those detected with higher-resolution NMR. In this study, diagnostic marker metabolites were identified for the smIEM disorders. In some cases, changes in metabolite profiles differentiated post-treatments and follow-ups while allowing for the establishment of different clinical states of a biochemical disorder. In addition, for the first time, a 1H-NMR-based biomarker profile was established for holocarboxylase synthase deficiency spectrum.
Development of Cell-Carrying Magnetic Microrobots with Bioactive Nanostructured Titanate Surface for Enhanced Cell Adhesion
Authors: Li Junyang , Fan Lei , Li Yanfang , Wei Tanyong , Wang Cheng , Li Feng , Tian Hua , Sun Dong
Published: December 2021

Cell-carrying magnet-driven microrobots are easily affected by blood flow or body fluids during transportation in the body, and thus cells often fall off from the microrobots. To reduce the loss of loaded cells, we developed a microrobot with a bioactive nanostructured titanate surface (NTS), which enhances cell adhesion. The microrobot was fabricated using 3D laser lithography and coated with nickel for magnetic actuation. Then, the microrobot was coated with titanium for the external generation of an NTS through reactions in NaOH solution. Enhanced cell adhesion may be attributed to the changes in the surface wettability of the microrobot and in the morphology of the loaded cells. An experiment was performed on a microfluidic chip for the simulation of blood flow environment, and result revealed that the cells adhered closely to the microrobot with NTS and were not obviously affected by flow. The cell viability and protein absorption test and alkaline phosphatase activity assay indicated that NTS can provide a regulatory means for improving cell proliferation and early osteogenic differentiation. This research provided a novel microrobotic platform that can positively influence the behaviour of cells loaded on microrobots through surface nanotopography, thereby opening up a new route for microrobot cell delivery.
Nanoribbon Biosensor in the Detection of miRNAs Associated with Colorectal Cancer
Authors: Ivanov Yuri D. , Goldaeva Kristina V. , Malsagova Kristina A. , Pleshakova Tatyana O. , Galiullin Rafael A. , Popov Vladimir P. , Kushlinskii Nikolay E. , Alferov Alexander A. , Enikeev Dmitry V. , Potoldykova Natalia V. , Archakov Alexander I.
Published: December 2021

A nanoribbon biosensor (NRBS) was developed to register synthetic DNAs that simulate and are analogous to miRNA-17-3p associated with colorectal cancer. Using this nanoribbon biosensor, the ability to detect miRNA-17-3p in the blood plasma of a patient diagnosed with colorectal cancer has been demonstrated. The sensing element of the NRBS was a nanochip based on a silicon-on-insulator (SOI) nanostructure. The nanochip included an array of 10 nanoribbons and was designed with the implementation of top-down technology. For biospecific recognition of miRNA-17-3p, the nanochip was modified with DNA probes specific for miRNA-17-3p. The performance of the nanochip was preliminarily tested on model DNA oligonucleotides, which are synthetic analogues of miRNA-17-3p, and a detection limit of ~10−17 M was achieved. The results of this work can be used in the development of serological diagnostic systems for early detection of colorectal cancer.
Static and Dynamic Optical Analysis of Micro Wrinkle Formation on a Liquid Surface
Authors: Saxena Antariksh , Tsakonas Costas , Chappell David , Cheung Chi , Edwards Andrew , Liang Haida , Sage Ian , Brown Carl
Published: December 2021

A spatially periodic voltage was used to create a dielectrophoresis induced periodic micro wrinkle deformation on the surface of a liquid film. Optical Coherence Tomography provided the equilibrium wrinkle profile at submicron accuracy. The dynamic wrinkle amplitude was derived from optical diffraction analysis during sub-millisecond wrinkle formation and decay, after abruptly increasing or reducing the voltage, respectively. The decay time constant closely followed the film thickness dependence expected for surface tension driven viscous levelling. Modelling of the system using numerical solution of the Stokes flow equations with electrostatic forcing predicted that wrinkle formation was faster than decay, in accord with observations.
Genomic Epidemiology of SARS-CoV-2 Divulge B.1, B.1.36, and B.1.1.7 as the Most Dominant Lineages in First, Second, and Third Wave of SARS-CoV-2 Infections in Pakistan
Authors: Basheer Atia , Zahoor Imran
Published: December 2021

The present study aims to investigate the genomic variability and epidemiology of SARS-CoV-2 in Pakistan along with its role in the spread and severity of infection during the three waves of COVID-19. A total of 453 genomic sequences of Pakistani SARS-CoV-2 were retrieved from GISAID and subjected to MAFFT-based alignment and QC check which resulted in removal of 53 samples. The remaining 400 samples were subjected to Pangolin-based genomic lineage identification. And to infer our SARS-CoV-2 time-scaled and divergence phylogenetic trees, 3804 selected global reference sequences plus 400 Pakistani samples were used for the Nextstrain analysis with Wuhan/Hu-1/2019, as reference genome. Finally, maximum likelihood based phylogenetic tree was built by using the Nextstrain and coverage map was created by employing Nextclade. By using the amino acid substitutions, the maximum likelihood phylogenetic trees were developed for each wave, separately. Our results reveal the circulation of 29 lineages, belonging to following seven clades G, GH, GR, GRY, L, O, and S in the three waves. From first wave, 16 genomic lineages of SARS-CoV-2 were identified with B.1(24.7%), B.1.36(18.8%), and B.1.471(18.8%) as the most prevalent lineages respectively. The second wave data showed 18 lineages, 10 of which were overlapping with the first wave suggesting that those variants could not be contained during the first wave. In this wave, a new lineage, AE.4, was reported from Pakistan for the very first time in the world. However, B.1.36 (17.8%), B.1.36.31 (11.9%), B.1.1.7 (8.5%), and B.1.1.1 (5.9%) were the major lineages in second wave. Third wave data showed the presence of nine lineages with Alpha/B.1.1.7 (72.7%), Beta/B.1.351 (12.99%), and Delta/B.1.617.2 (10.39%) as the most predominant variants. It is suggested that these VOCs should be contained at the earliest in order to prevent any devastating outbreak of SARS-CoV-2 in the country.
The Role of Aragonite in Producing the Microstructural Diversity of Serpulid Skeletons
Authors: Vinn Olev
Published: December 2021

Aragonite plays an important role in the biomineralization of serpulid polychaetes. Aragonitic structures are present in a wide range of serpulid species, but they mostly belong to one clade. Aragonitic structures are present in a wide range of marine environments, including the deep ocean. Aragonitic tube microstructures were studied using a scanning electron microscope. X-ray powder diffraction was used to identify the aragonite. Aragonite is used to build five different types of microstructures in serpulid tubes. The most common aragonitic irregularly oriented prismatic structure (AIOP) is also, evolutionarily, the most primitive. Some aragonitic microstructures, such as the spherulitic prismatic (SPHP) structure, have likely evolved from the AIOP structure. Aragonitic microstructures in serpulids are far less numerous than calcitic microstructures, and they lack the complexity of advanced calcitic microstructures. The reason why aragonitic microstructures have remained less evolvable than calcitic microstructures is currently unknown, considering their fit with the current aragonite sea conditions (Paleogene–recent).
Real-Time Electrical Conductivity Monitoring and Correlation with Sulfate Release and Acid Mine Drainage Potential from a Gold/Silver Paste Tailing Storage
Authors: Leiva Eduardo , Cayazzo María , Torres Mario
Published: December 2021

Safe disposal of tailings as high-density thickened tailings or paste tailings can reduce the environmental risks of conventional tailings deposits, reduce water use, minimize tailings storage facility footprints, reduce the potential for acid mine drainage (AMD), and minimize risks of failure, among other advantages. In the dewatering process, the addition of flocculants is key to improving the sedimentation of the tailings and the formation of a compact paste. Despite the environmental and operational advantages of using paste tailings, it is not clear how the chemical nature of coagulants and flocculants could influence the discharge of toxic elements (salts and metals) from tailings after storage. In this study, we show the results of the real-time evaluation of the release of polluting runoffs from a paste tailings deposit. To do this, we analyzed paste tailing samples for AMD potential through static and kinetic tests and monitored the electrical conductivity and real-time pH, evaluating the correlation with the sulfate in the thickener and downstream from the tailings deposit. Tailing samples have low sulfur content (<2%) and low acid-generating potential. Moreover, there is no evidence of a significant positive correlation (Pearson’s coefficient r < 0.8) between the sulfate concentrations with the pH or EC. Thus, the chemical nature of the paste tailings prior to discharge has no direct impact on the release of sulfate-rich runoffs from the tailings after its storage. This indicates that the tailings paste at the evaluated site is chemically stable in the short term.
Solid Dosage Forms of Biopharmaceuticals in Drug Delivery Systems Using Sustainable Strategies
Authors: Costa Clarinda , Casimiro Teresa , Corvo Maria Luísa , Aguiar-Ricardo Ana
Published: December 2021

Drug delivery systems (DDS) often comprise biopharmaceuticals in aqueous form, making them susceptible to physical and chemical degradation, and therefore requiring low temperature storage in cold supply and distribution chains. Freeze-drying, spray-drying, and spray-freeze-drying are some of the techniques used to convert biopharmaceuticals-loaded DDS from aqueous to solid dosage forms. However, the risk exists that shear and heat stress during processing may provoke DDS damage and efficacy loss. Supercritical fluids (SCF), specifically, supercritical carbon dioxide (scCO2), is a sustainable alternative to common techniques. Due to its moderately critical and tunable properties and thermodynamic behavior, scCO2 has aroused scientific and industrial interest. Therefore, this article reviews scCO2-based techniques used over the year in the production of solid biopharmaceutical dosage forms. Looking particularly at the use of scCO2 in each of its potential roles—as a solvent, co-solvent, anti-solvent, or co-solute. It ends with a comparison between the compound’s stability using supercritical CO2-assisted atomization/spray-drying and conventional drying.
Current Photoactive Molecules for Targeted Therapy of Triple-Negative Breast Cancer
Authors: Dias Cristina J. , Helguero Luisa , Faustino Maria Amparo F.
Published: December 2021

Cancer is the second leading cause of death worldwide; therefore, there is an urgent need to find safe and effective therapies. Triple-negative breast cancer (TNBC) is diagnosed in ca. 15–20% of BC and is extremely aggressive resulting in reduced survival rate, which is mainly due to the low therapeutic efficacy of available treatments. Photodynamic therapy (PDT) is an interesting therapeutic approach in the treatment of cancer; the photosensitizers with good absorption in the therapeutic window, combined with their specific targeting of cancer cells, have received particular interest. This review aims to revisit the latest developments on chlorin-based photoactive molecules for targeted therapy in TNBC. Photodynamic therapy, alone or combined with other therapies (such as chemotherapy or photothermal therapy), has potential to be a safe and a promising approach against TNBC.
Pineapple Leaf Phenols Attenuate DSS-Induced Colitis in Mice and Inhibit Inflammatory Damage by Targeting the NF-κB Pathway
Authors: Chen Yang , Niu Yaoyun , Hao Wenhui , Zhang Wanqiu , Lu Jinghua , Zhou Jin , Du Lijun , Xie Weidong
Published: December 2021

Colitis is not fully curable, although currently, some treatment options are being adopted. In this study, we investigated the effects of pineapple leaf phenols (PLPs), natural phenol products from pineapple leaves, on DSS-induced colitis in mice. The results showed that PLPs dramatically decreased the inflammatory response by inhibiting NF-κB activation and the secretion of pro-inflammatory factors. Moreover, PLPs provided protection against DSS-induced acute colitis by maintaining epithelial integrity. Caffeic and P-coumaric acids had similar effects and could be the active components responsible for PLPs’ effect on colitis. These results indicate that the oral administration of PLPs might be considered as a therapeutic strategy in the treatment of patients with colitis. However, further research on clinical applications and the exact effect of PLPs on colitis is required.