bluuebunny/crossref_metadata_2025 huggingface dataset:
Using a Hand-Held Icterometer to Screen for Neonatal Jaundice: Validation, Feasibility, and Acceptability of the Bili-RulerTM in Kumasi, Ghana
Authors: Bakari Ashura , Wolski Ann V. , Otoo Benjamin , Amoah Rexford , Nakua Emmanuel K. , Jacovetty Jacob , Kaselitz Elizabeth , Compton Sarah D. , Moyer Cheryl A.
Published: January 2025

Background: Neonatal jaundice (NNJ) remains a leading cause of newborn mortality in much of sub-Saharan Africa. We sought to examine the validity of using a hand-held icterometer as a screening tool to determine which newborns need further assessment. Additionally, we sought to assess the feasibility of its use among mothers. Methods: We recruited and trained healthcare workers at one large district hospital in Ghana to use a hand-held icterometer known as the Bili-RulerTM. We recruited mothers of 341 newborns aged 0 to 2 weeks at the same hospital. Mothers watched a standardized training video, after which they blanched the skin of the newborn’s nose and compared it with the yellow shades numbered one to six on the icterometer. Each newborn was also assessed with a transcutaneous bilirubin meter (TCB). Research assistants and health care workers screened the same newborns, recorded their scores separately, and were blinded to each other’s readings. In the second phase of this study, we recruited 100 new mothers to take the Bili-Ruler home with them, instructing them to check their newborns twice daily. We interviewed them 1–2 weeks later to determine the acceptability and feasibility of its use. Results: Out of 341 newborns screened, 20 had elevated TCB indicative of hyperbilirubinemia. Healthcare workers’ Bili-Ruler ratings had a strong and significant correlation with TCB scores, as did the ratings of researchers and mothers. When comparing Bili-Ruler scores against TCB, sensitivity across all three raters was 80% (95% CI 75.6–84.3), specificity ranged from 61.1% (healthcare providers) to 66.7% (researchers), positive predictive value ranged from 11.4% (healthcare providers) to 13.0% (researchers), and negative predictive value was 98.0% or higher across all raters. Area under the ROC curve ranged from 0.71 for healthcare providers to 0.73 for researchers. Mothers AUC was 0.72. In terms of acceptability and feasibility, the Bili-Ruler was widely accepted by the mothers and family. In total, 98% of mothers reported using it, and 90.8% used it 3 or more days in the first week after birth. Moreover, 89.8% used it more than twice per day. Conclusions: A hand-held, low-tech icterometer is an important potential mechanism for improving early jaundice identification in low-resource settings. Further studies using larger sample sizes with a higher prevalence of hyperbilirubinemia are warranted.
Phenotypic Antibiotic Resistance Patterns of Escherichia coli Isolates from Clinical UTI Samples and Municipal Wastewater in a Grenadian Community
Authors: Matthew-Bernard Makeda , Farmer-Diaz Karla , Dolphin-Bond Grace , Matthew-Belmar Vanessa , Cheetham Sonia , Mitchell Kerry , Macpherson Calum N. L. , Ramos-Nino Maria E.
Published: January 2025

Antimicrobial resistance (AMR) is a growing global health threat. This study investigated antibiotic resistance in E. coli isolates from municipal wastewater (86 isolates) and clinical urinary tract infection (UTI) cases (34 isolates) in a Grenadian community, using data from January 2022 to October 2023. Antibiogram data, assessed per WHO guidelines for Critically Important antimicrobials (CIA), showed the highest resistance levels in both clinical and wastewater samples for ampicillin, followed by amoxicillin/clavulanic acid and nalidixic acid, all classified as Critically Important. Similar resistance was observed for sulfamethoxazole-trimethoprim (highly important) in both groups, with nitrofurantoin showing resistance in the important category. According to the WHO AWaRe classification, ampicillin (ACCESS group) had the highest resistance, while nitrofurantoin had the lowest across all samples. The WATCH group antibiotics, cefuroxime and cefoxitin, showed comparable resistance levels, whereas aztreonam from the RESERVE group (tested only in wastewater) was 100% sensitive. Multiple Antibiotic Resistance (MAR) index analysis revealed that 7% of wastewater and 38.2% of clinical samples had MAR values over 0.2, indicating prior antibiotic exposure in clinical isolates. These parallel patterns in wastewater and clinical samples highlight wastewater monitoring as a valuable tool for AMR surveillance, supporting antibiotic stewardship through ongoing environmental and clinical assessment.
Explaining the Correlates of the Multi-Theory Model (MTM) of Health Behavior Change in Visual (Structural) Colorectal Cancer Screening Examinations
Authors: Awan Asma T. , Grigsby Timothy J. , Johansen Christopher , Dai Chia-Liang , Sharma Manoj
Published: January 2025

Colorectal cancer (CRC) ranks third in terms of global cancer prevalence and is the second most common cause of cancer-related mortality. Although CRC rates are decreasing in the United States, inequalities still exist despite the effectiveness of invasive screening methods, such as colonoscopy, flexible sigmoidoscopy, and computed tomography (CT) colonography in detecting colorectal cancer. Many current interventions promoting CRC screening do not utilize a modern theory-based approach, which has led to the low utilization of these screening methods. This cross-sectional study aims to address the lack of theory-based treatments for promoting visual CRC screening examinations by applying the multi-theory model (MTM) of health behavior change to explicate the health-related factors for individuals to seek visual colorectal cancer screening examinations for CRC screening. A 57-item validated questionnaire assessing MTM constructs and CRC screening was administered online. The survey questionnaire was administered to a sample of 640 adults from the United States. The participants were between the ages of 45 and 75 years. Hierarchical multiple regression was used to assess the relationship between MTM constructs with the initiation and sustenance of CRC screening behaviors. Out of the total participants in this nationwide sample, 71.4% (n = 457) reported that they had undergone a visual CRC screening examination. MTM subscales, specifically participatory dialogue, changes in the physical environment along with age, recommendation for CRC screening from a healthcare provider, and previous experience with colonoscopy, were found to be significant factors in predicting the initiation of visual CRC screening behavior. These factors accounted for 22% of the variation in initiation among this group (R2 = 0.222, F = 3.521, p < 0.001). The MTM can be a valuable framework for designing educational media, information media, social media platforms, and clinical interventions to promote visual colorectal cancer screening examinations.
A New Construction Method for Rectangular Cartograms
Authors: Wang Lina , Yuan Haoxun , Li Xiang , Lu Pengfei , Li Yaru
Published: January 2025

The rectangular cartogram is a geospatial visualization method that blends the characteristics of maps and charts. By simplifying geographic regions into rectangles and using the area of each rectangle to represent statistical data, it enables efficient geovisualization. This paper summarizes and analyzes the advantages and limitations of two main approaches used in current rectangular cartogram construction algorithms. To address the issues of high computational cost and inadequate preservation of adjacency and relative positional relationships in existing algorithms, we propose and implement a new rectangular cartogram construction algorithm. This algorithm simplifies the layout computation process while ensuring that the adjacency and relative positional relationships between regions during the layout generation process have only minor errors. In adjusting rectangle areas to match attribute values, the algorithm adopts a “region-by-region placement” strategy, ensuring that errors in area accuracy remain within a small range, while also keeping errors in adjacency and relative positional relationships minimal. Finally, by comparing the results of our algorithm with those of existing algorithms using real-world data with varying distribution characteristics, we demonstrate its effectiveness. The results show that the proposed algorithm not only improves computational efficiency but also effectively displays the adjacency and relative positional relationships between regions.
Non-Invasive Determination of the Paternal Inheritance in Pregnancies at Risk for β-Thalassaemia by Analyzing Cell-Free Fetal DNA Using Targeted Next-Generation Sequencing
Authors: Byrou Stefania , Brouwer Rutger W. W. , Tomazou Marios , Tamana Stella , Kountouris Petros , Lederer Carsten W. , Petrou Miranda , Ozgur Zeliha , den Dekker Xander , Azmani Zakia , Christou Soteroula , Makariou Christiana , Kleanthous Marina , IJcken Wilfred F. J. van , Papasavva Thessalia
Published: January 2025

Non-invasive prenatal testing (NIPT) has been widely adopted for the screening of chromosomal abnormalities; however, its adoption for monogenic disorders, such as β-thalassaemia, has proven challenging. Haemoglobinopathies are the most common monogenic disorders globally, with β-thalassaemia being particularly prevalent in Cyprus. This study introduces a non-invasive prenatal haplotyping (NIPH) assay for β-thalassaemia, utilizing cell-free DNA (cfDNA) from maternal plasma. The assay determines paternal inheritance by analyzing highly heterozygous single-nucleotide variants (SNVs) in the β-globin gene cluster. To identify highly heterozygous SNVs in the population, 96 randomly selected samples were processed using Illumina DNA-prep NGS chemistry. A custom, high-density NGS genotyping panel, named HAPLONID, was designed with 169 SNVs, including 15 common pathogenic ones. The AmpliSeq for Illumina assay was then applied to cfDNA to evaluate the panel’s efficiency in performing NIPT for β-thalassaemia. Analysis revealed 219 highly polymorphic SNVs, and the sequencing of 17 families confirmed successful paternal allele determination. The NIPH assay demonstrated 100% success in diagnostic interpretation. This study achieved the advancement of an integrated NGS-NIPT assay for β-thalassaemia, bringing it one step closer to being a diagnostic assay and thereby enabling a reduction in the number of risky invasive prenatal sampling procedures in Cyprus and elsewhere.
Protocadherin-7 Regulates Monocyte Migration Through Regulation of Small GTPase RhoA and Rac1
Authors: Kim Hyunsoo , Takegahara Noriko , Choi Yongwon
Published: January 2025

Protocadherin-7 (Pcdh7) is a member of the non-clustered protocadherin δ1 subgroup within the cadherin superfamily. Pcdh7 has been shown to control osteoclast differentiation via the protein phosphatase 2A (PP2A)–glycogen synthase kinase-3β (GSK3β)–small GTPase signaling axis. As protocadherins serve multiple biological functions, a deeper understanding of Pcdh7’s biological features is valuable. Using an in vitro mouse monocyte cell culture system, we demonstrate that Pcdh7 plays a role in regulating monocyte migration by modulating the small GTPases RhoA and Rac1. Pcdh7-deficient (Pcdh7−/−) bone marrow-derived monocytes exhibited impaired migration along with the reduced activation of RhoA and Rac1. This impaired migration was rescued by transduction with constitutively active forms of RhoA and Rac1. Treatment with the PP2A-specific activator DT-061 enhanced cell migration, whereas treatment with the GSK3β-specific inhibitor AR-A014418 inhibited migration in wild-type monocytes. In contrast, treatment with DT-061 failed to restore the impaired migration in Pcdh7−/− monocytes. These findings suggest the involvement of PP2A and GSK3β in monocyte migration, although the forced activation of PP2A alone is insufficient to restore impaired migration in Pcdh7−/− monocytes. Taken together, these results indicate that Pcdh7 regulates monocyte migration through the activation of RhoA and Rac1. Given the pivotal role of cell migration in both physiological and pathological processes, our findings provide a foundation for future research into therapeutic strategies targeting Pcdh7-regulated migration.
Conditional Split Inteins: Adaptable Tools for Programming Protein Functions
Authors: Shepherd Callum , Lawson-Williams Makeba , Holland Alexandria , Bello Adebayo J. , Sexton Darren W. , Olorunniji Femi J.
Published: January 2025

Split inteins are biological mechanisms for the operation of the spatiotemporal control of protein activities. They function through protein trans-splicing, in which their N- and C-terminal fragments are expressed contiguously with two protein halves. The subsequent self-excision upon recognition of the complimentary fragment yields a mature, complete, and functional protein. The conditional regulation of protein splicing through environmental factors or the attachment of regulatory modules can be used to determine when and where a protein will operate, providing potential novel approaches for engineering biology applications. This review will discuss current split intein applications and the mechanistic basis for novel species classification. These considerations can provide guidance in intein and extein engineering through activation strategies, in the design of spatial arrangements, and in taking advantage of unique reaction environments. This can pave the way for the future implementation of novel split intein discoveries and the selection of appropriate intein species and aid in designing novel protein engineering strategies.
Localization and Molecular Cloning of the ASMT Gene for Melatonin Synthesis in Pigs
Authors: Yan Laiqing , Li Guangdong , Deng Shoulong , Wang Likai , Wang Yiwei , Shen Zixia , Yin Depeng , Ji Pengyun , Wang Bingyuan , Liu Guoshi
Published: January 2025

Melatonin is synthesized in multiple tissues and organs of pigs, and existing studies have shown the presence of the melatonin-synthesizing enzyme ASMT protein. However, the genomic information for the ASMT gene has been lacking. The aim of this study was to locate the genomic information of the ASMT gene in pigs using comparative genomics analysis and then obtain the coding region information through molecular cloning. First, using the NCBI Genome Data Viewer, we found that in most animals, the AKAP17A gene is often located next to the ASMT gene, with both genes arranged in the same direction. Similarly, the P2RY8 gene is commonly adjacent to the ASMTL gene, also in the same orientation. We also discovered that the ASMTL gene is frequently adjacent to the ASMT gene and arranged in the opposite direction. Using the “three-point localization” principle, we inferred the position of the ASMT gene based on the coordinates of AKAP17A and ASMTL in pigs. Our results revealed that on the pig X chromosome, a gene called LOC110258194 is located next to the AKAP17A and ASMTL genes, and its arrangement aligns with the ASMT gene in other species. Additionally, Ensembl contains a gene, ENSSSCG00000032659, at the same position, with completely overlapping exons, though it is not annotated as ASMT. Further analysis using the TreeFam tool from EMBL-EBI and the CDD tool from NCBI revealed that LOC110258194 and ENSSSCG00000032659 do not contain the typical Maf domain of ASMTL and, thus, should not be annotated as ASMTL, but rather as the ASMT gene. Using a slow-down PCR method for high-GC content genes, we successfully cloned the full CDS region of the pig ASMT gene and identified a new transcript missing Exon 6 and Exon 7. This transcript was submitted to NCBI and assigned the GenBank accession number MW847601. Our results represent the first successful localization of the ASMT gene in pigs, the first cloning of the ASMT gene’s coding region, and the first discovery of a new transcript of the pig ASMT gene.
Potential Use of Selected Natural Compounds with Anti-Biofilm Activity
Authors: Fydrych Dagmara , Jeziurska Jagoda , Wełna Jana , Kwiecińska-Piróg Joanna
Published: January 2025

Antibiotic resistance in microorganisms is an escalating global concern, exacerbated by their formation of biofilms, which provide protection through an extracellular matrix and communication via quorum sensing, enhancing their resistance to treatment. This situation has driven the search for alternative approaches, particularly those using natural compounds. This study explores the potential of phytochemicals, such as quercetin, apigenin, arbutin, gallic acid, proanthocyanidins, and rutin, known for their antibacterial properties and ability to inhibit biofilm formation and disrupt mature biofilms. The methods used in this study included a comprehensive review of current literature assessing the bioavailability, distribution, and effective concentrations of these compounds in treating biofilm-associated infections. The results indicate that these phytochemicals exhibit significant antibacterial effects, reduce biofilm’s structural integrity, and inhibit bacterial communication pathways. Moreover, their potential use in combination with existing antibiotics may enhance therapeutic outcomes. The findings support the conclusion that phytochemicals offer promising additions to anti-biofilm strategies and are capable of complementing or replacing conventional treatments, with appropriate therapeutic levels and delivery mechanisms being key to their effectiveness. This insight underscores the need for further research into their clinical applications for treating infections complicated by biofilms.
Evidence for the Worldwide Distribution of a Bile Salt Hydrolase Gene in Enterococcus faecium Through Horizontal Gene Transfer
Authors: Kusada Hiroyuki , Tamaki Hideyuki
Published: January 2025

Bile salt hydrolase (BSH), a probiotic-related enzyme with cholesterol-assimilating and anti-hypercholesterolemic abilities, has been isolated from intestinal bacteria; however, BSH activity of bacteria in bile-salt-free (non-intestinal) environments is largely unknown. Here, we aimed to identify BSH from non-intestinal Enterococcus faecium and characterize its enzymatic function. We successfully isolated a plasmid-encoded bsh (efpBSH) from E. faecium, and the recombinant EfpBSH showed BSH activity that preferentially hydrolyzed taurine-conjugated bile salts, unlike the activity of known BSHs. EfpBSH functioned optimally at pH 4.0 and 50 °C. EfpBSH exhibited very low amino acid sequence similarity (48.46%) to EfBSH from E. faecalis T2 isolated from human urine, although 241 sequences with 100% identity to EfpBSH were found in both plasmids and chromosomes of E. faecium strains inhabiting intestinal and non-intestinal environments. Phylogenetically, EfpBSH was not affiliated with any known BSH phylogroup and was clearly distinguished from previously identified BSHs from intestinal lactic acid bacteria. Our genome database analysis demonstrated that horizontal gene transfer causes global efpBSH distribution among E. faecium strains in various environments (soil, water, and intestinal samples) and geographical regions (Asia, Africa, Europe, North America, South America, and Australia/Oceania). Overall, our findings are the first to indicate that BSH is not an intestine-specific enzyme and that hitherto-overlooked probiotic candidates with BSH activity can exist in diverse environments.
Mechanistic Insights into Melatonin’s Antiarrhythmic Effects in Acute Ischemia-Reperfusion-Injured Rabbit Hearts Undergoing Therapeutic Hypothermia
Authors: Lee Hui-Ling , Chang Po-Cheng , Wo Hung-Ta , Chou Shih-Chun , Chou Chung-Chuan
Published: January 2025

The electrophysiological mechanisms underlying melatonin’s actions and the electrophysiological consequences of superimposed therapeutic hypothermia (TH) in preventing cardiac ischemia-reperfusion (IR) injury-induced arrhythmias remain largely unknown. This study aimed to unveil these issues using acute IR-injured hearts. Rabbits were divided into heart failure (HF), HF+melatonin, control, and control+melatonin groups. HF was induced by rapid right ventricular pacing. Melatonin was administered orally (10 mg/kg/day) for four weeks, and IR was created by 60-min coronary artery ligation and 30-min reperfusion. The hearts were then excised and Langendorff-perfused for optical mapping studies at normothermia, followed by TH. Melatonin significantly reduced ventricular fibrillation (VF) maintenance. In failing hearts, melatonin reduced the spatially discordant alternans (SDA) inducibility mainly by modulating intracellular Ca2+ dynamics via upregulation of sarcoplasmic reticulum Ca2+-ATPase (SERCA2a) and calsequestrin 2 and attenuating the downregulation of phosphorylated phospholamban protein expression. In control hearts, melatonin improved conduction slowing and reduced dispersion of action potential duration (APDdispersion) by upregulating phosphorylated connexin 43, attenuating the downregulation of SERCA2a and phosphorylated phospholamban and attenuating the upregulation of phosphorylated Ca2+/calmodulin-dependent protein kinase II. TH significantly retarded intracellular Ca2+ decay slowed conduction, and increased APDdispersion, thereby facilitating SDA induction, which counteracted the beneficial effects of melatonin in reducing VF maintenance.
Two- and Three-Dimensional In Vitro Models of Parkinson’s and Alzheimer’s Diseases: State-of-the-Art and Applications
Authors: Solana-Manrique Cristina , Sánchez-Pérez Ana María , Paricio Nuria , Muñoz-Descalzo Silvia
Published: January 2025

In vitro models play a pivotal role in advancing our understanding of neurodegenerative diseases (NDs) such as Parkinson’s and Alzheimer’s disease (PD and AD). Traditionally, 2D cell cultures have been instrumental in elucidating the cellular mechanisms underlying these diseases. Cultured cells derived from patients or animal models provide valuable insights into the pathological processes at the cellular level. However, they often lack the native tissue environment complexity, limiting their ability to fully recapitulate their features. In contrast, 3D models offer a more physiologically relevant platform by mimicking the 3D brain tissue architecture. These models can incorporate multiple cell types, including neurons, astrocytes, and microglia, creating a microenvironment that closely resembles the brain’s complexity. Bioengineering approaches allow researchers to better replicate cell–cell interactions, neuronal connectivity, and disease-related phenotypes. Both 2D and 3D models have their advantages and limitations. While 2D cultures provide simplicity and scalability for high-throughput screening and basic processes, 3D models offer enhanced physiological relevance and better replicate disease phenotypes. Integrating findings from both model systems can provide a better understanding of NDs, ultimately aiding in the development of novel therapeutic strategies. Here, we review existing 2D and 3D in vitro models for the study of PD and AD.
A Narrative Review of the Interplay Between Carbohydrate Intake and Diabetes Medications: Unexplored Connections and Clinical Implications
Authors: Mphasha Mabitsela Hezekiel , Vagiri Rajesh
Published: January 2025

This narrative review examines the dynamic interplay between carbohydrate intake and diabetes medications, highlighting their combined molecular and clinical effects on glycemic control. Carbohydrates, a primary energy source, significantly influence postprandial glucose regulation and necessitate careful coordination with pharmacological therapies, including insulin, metformin, glucagon-like peptide (GLP-1) receptor agonists, and sodium-glucose cotransporter-2 (SGLT2) inhibitors. Low-glycemic-index (GI) foods enhance insulin sensitivity, stabilize glycemic variability, and optimize medication efficacy, while high-GI foods exacerbate glycemic excursions and insulin resistance. Continuous glucose monitoring (CGM) offers real-time insights to tailor dietary and pharmacological interventions, improving glycemic outcomes and reducing complications. Despite advancements, gaps persist in understanding nutrient–drug interactions, particularly with emerging antidiabetic agents. This review underscores the need for integrating carbohydrate-focused dietary strategies with pharmacotherapy to enhance diabetes management. Future research should prioritize clinical trials leveraging CGM technology to explore how glycemic index, glycemic load, and carbohydrate quality interact with newer diabetes medications. Such studies can refine evidence-based recommendations, support individualized care plans, and improve long-term outcomes. Addressing systemic barriers, such as limited access to dietitians and CGM technology in underserved regions, is critical for equitable care. Expanding the roles of community health workers and training healthcare providers in basic nutrition counseling can bridge gaps, promoting sustainable and inclusive diabetes management strategies. These efforts are essential for advancing personalized, effective, and equitable care for individuals with diabetes.
Mitochondrial microRNAs: Key Drivers in Unraveling Neurodegenerative Diseases
Authors: Yashooa Raya Kh. , Duranti Elisa , Conconi Donatella , Lavitrano Marialuisa , Mustafa Suhad A. , Villa Chiara
Published: January 2025

MicroRNAs (miRNAs) are a class of small non-coding RNAs (ncRNAs) crucial for regulating gene expression at the post-transcriptional level. Recent evidence has shown that miRNAs are also found in mitochondria, organelles that produce energy in the cell. These mitochondrial miRNAs, also known as mitomiRs, are essential for regulating mitochondrial function and metabolism. MitomiRs can originate from the nucleus, following traditional miRNA biogenesis pathways, or potentially from mitochondrial DNA, allowing them to directly affect gene expression and cellular energy dynamics within the mitochondrion. While miRNAs have been extensively investigated, the function and involvement of mitomiRs in the development of neurodegenerative disorders like Alzheimer’s disease, Parkinson’s disease, and amyotrophic lateral sclerosis remain to be elucidated. This review aims to discuss findings on the role of mitomiRs in such diseases and their potential as therapeutic targets, as well as to highlight future research directions.
TempoGRAPHer: Aggregation-Based Temporal Graph Exploration
Authors: Tsoukanara Evangelia , Koloniari Georgia , Pitoura Evaggelia
Published: January 2025

Graphs offer a generic abstraction for modeling entities and the interactions and relationships between them. Most real-world graphs, such as social and cooperation networks, evolve over time, and exploring their evolution may reveal important information. In this paper, we present TempoGRAPHer, a system for analyzing and visualizing the evolution of temporal attributed graphs. TempoGRAPHer supports both temporal and attribute aggregation. It also allows graph exploration by identifying periods of significant growth, shrinkage, or stability. Temporal exploration is supported by two complementary strategies, namely skyline- and interaction-based exploration. Skyline-based exploration provides insights into the overall trends in the evolution, while interaction-based exploration offers a closer look at specific parts of the graph evolution history where significant changes occurred. We present experimental results demonstrating the efficiency of TempoGRAPHer. Additionally, we showcase the usefulness of our system in understanding graph evolution by presenting detailed scenarios, including exploring the evolution of a real contact network between primary school students and analyzing the collaborations in a co-authorship network between authors of the same gender over time.
Age-Stage, Two-Sex Life Table of Leptinotarsa decemlineata (Coleoptera: Chrysomelidae) Experiencing Cadmium Stress
Authors: He Bingyu , Zhang Jiebo , Hu Yang , Zhang Yi , Wang Jianan , Li Chao
Published: January 2025

Cadmium in agricultural soils has emerged as a substantial threat to crop health and yields through its bioaccumulation along the food chain, with further repercussions for the growth, development, and population dynamics of herbivorous insects. In this study, potted potato plants were treated with Cd2+ solutions at concentrations of 0 mg/kg, 30 mg/kg, 60 mg/kg, 90 mg/kg, and 120 mg/kg. Colorado potato beetles (Leptinotarsa decemlineata) were fed on potato leaves exposed to these varying concentrations of cadmium, and the effects on their growth and development were assessed. The results revealed that: 1. The developmental period, pupal stage, and pre-oviposition period of the first-instar larvae of L. decemlineata feeding on cadmium-contaminated leaves was significantly prolonged, while both the lifespan and fecundity were reduced. 2. Key population parameters, including the innate rate of increase (r), finite rate of increase (λ), net reproductive rate (R0), and gross reproduction rate (GRR), were evidently lower in cadmium-exposed L. decemlineata, while the average generation time did not show a significant difference. 3. Cadmium exposure also resulted in a remarkable reduction in survival and reproductive rates at specific life stages, along with an increase in the incidence of deformations in newly emerged adults. These findings underscore the detrimental effects of cadmium on both crop health and pest populations. This study holds valuable implications for more effective implementation of pest control strategies in the future, offering robust scientific evidence to support the safeguarding of food security.
Differences in Arrhythmia Detection Between Harvard Step Test and Maximal Exercise Testing in a Paediatric Sports Population
Authors: Bianco Massimiliano , Sollazzo Fabrizio , Pella Riccardo , Vicentini Saverio , Ciaffoni Samuele , Modica Gloria , Monti Riccardo , Cammarano Michela , Zeppilli Paolo , Palmieri Vincenzo
Published: January 2025

BACKGROUND: Sport practice may elevate the risk of cardiovascular events, including sudden cardiac death, in athletes with undiagnosed heart conditions. In Italy, pre-participation screening includes a resting ECG and either the Harvard Step Test (HST) or maximal exercise testing (MET), but the relative efficacy of the latter two tests for detecting arrhythmias and heart conditions remains unclear. METHODS: This study examined 511 paediatric athletes (8–18 years, 76.3% male) without known cardiovascular, renal, or endocrine diseases. All athletes underwent both HST and MET within 30 days. Absolute data and data relative to theoretical peak heart rates, arrhythmias (supraventricular and ventricular) and cardiovascular diagnoses were collected. RESULTS: HST resulted in a lower peak heart rate than MET (181.1 ± 9.8 vs. 187.5 ± 8.1 bpm, p < 0.001), but led to the detection of more supraventricular (18.6% vs. 13.1%, p < 0.001) and ventricular (30.5% vs. 22.7%, p < 0.001) arrhythmias, clustering during recovery (p = 0.014). This pattern was significant in males but not females. Among athletes diagnosed with cardiovascular diseases (22.3%), HST identified more ventricular arrhythmias (26.3% vs. 18.4%, p = 0.05), recovery-phase arrhythmias (20.2% vs. 14.0%, p = 0.035), and polymorphic arrhythmias (6.1% vs. 1.8%, p = 0.025). CONCLUSIONS: HST detects arrhythmias more effectively than MET in young male athletes, especially during recovery. More ventricular arrhythmias were highlighted even in athletes with cardiovascular conditions.
Comorbidities Associated with Vitiligo: Results from the EpiChron Cohort
Authors: Clemente Hernández Beatriz , Muelas Rives Itziar , Gracia Cazaña Tamara , Álvarez Salafranca Marcial , Poblador-Plou Beatriz , Laguna-Berna Clara , Moreno Juste Aida , Gimeno-Miguel Antonio , Gilaberte Yolanda
Published: January 2025

Background: Vitiligo is a pigmentation disorder that impacts approximately 0.5% to 2% of the global population. Growing interest surrounds the comorbidities associated with vitiligo. This study aimed to describe the socio-demographic characteristics of the patients with vitiligo in Aragón (Spain) and to investigate their associated comorbidities. Methods: A retrospective observational study was conducted using clinical data from individuals in the EpiChron Cohort (reference population of 1.3 million) who were diagnosed with vitiligo between 1 January and 31 December 2019. The prevalence of chronic comorbidities was calculated using logistic regression models, obtaining the odds ratio (OR) of each comorbidity (dependent variable) according to the presence or absence of vitiligo (independent variable). We used a cut-off point for a statistical significance of p-value < 0.05. Results: In total, 218 patients diagnosed with vitiligo were analyzed. The mean age was 44.0 years, and 56.42% were female. The largest proportion of patients (34.86%) were aged between 18 and 44 years. Among all vitiligo patients included, 71.5% presented multimorbidity, with an average of 3.21 diagnosed comorbidities. The conditions most frequently associated with vitiligo included thyroid disorders (OR: 3.01, p < 0.001), ocular and hearing abnormalities (OR: 1.54, p < 0.020), inflammatory skin disorders (OR: 2.21, p < 0.001), connective tissue diseases (OR: 1.84, p < 0.007), lower respiratory tract diseases (OR: 1.78, p < 0.014), urinary tract infections (OR: 1.69, p < 0.032), and cardiac arrhythmias (OR 1.84, p < 0.034). Conclusions: This research highlights the importance of understanding the broader health implications of vitiligo and provides a foundation for further exploration into the complex interplay between this dermatologic condition and a diverse range of comorbidities.
Hydrophilic Polymer Embolization—A Scoping Review of the Available Literature with Focus on Organ Involvement and Outcomes
Authors: Abdulrasak Mohammed , Kadim Haydar , Someili Ali , Mohrag Mostafa
Published: January 2025

Background: Hydrophilic polymer embolization (HPE) is a scarcely reported complication associated with endovascular procedures where the hydrophilic coating dislodges and disseminates to more distal vascular beds, leading to ischemic complications. The aim of this study is to assess the clinical outcomes associated with HPE in the literature and try to quantify it in a scoping manner. Methods: All reports with regard to HPE in the PubMed database where clinical data were available were included. Reports were excluded if no clinical data were available and only histopathological descriptions are available, if the language of the report was not in English, and if access could not be obtained to that specific report. Results: A total of 60 publications containing 111 patients were identified. The majority (N = 45, 75%) of the publications were “single-patient” case reports. An overwhelming minority of the reports reported underlying hypertension (N = 27, 45.0%) and ischemic heart disease (N = 28, 46.7%). The most common implicated procedures for HPE occurence were cardiac procedures (N = 28, 46.7%), intracranial procedures (N = 13, 21.7%) and aortic procedures (N = 10, 16.7%). Steroids were trialled in nine (15%) of the reports, mainly for HPE to the CNS (7/9), with no mortality in that specific group. However, HPE-related mortality, identified in 48/111 patients, was largely due to HPE with pulmonary and cardiac involvement (combined 36/48 of all deaths). Conclusions: HPE seems to be a rare occurrence, although low-quality evidence (mainly case reports) comprises most of the research on the subject. Fatal outcomes seem relatively common, and steroid therapy may be trialled in select cases. Further research, potentially through prospective registry studies may aid in providing more knowledge on HPE.
The Effect of Visual Feedback on Plaque Control: An RCT Comparing Toothbrushing Methods in Periodontitis Patients
Authors: Tytgat Manon , Glibert Maarten , Callens Jeroen , Lamoral Sabine , Van Gyseghem Paulien , Decabooter Fien , Christiaens Véronique
Published: January 2025

Background/Objectives: As gingivitis prevalence is closely related to plaque accumulation, effective oral hygiene is mandatory for maintaining healthy gingival tissues. The aim of this study was to evaluate the impact of different toothbrushing methods (a manual toothbrush (group 1 = MTB, the control); an electric toothbrush (group 2 = ETB); and an electric toothbrush with daily visual feedback (group 3 = ETBV)) on the plaque levels and periodontal health in patients after receiving initial periodontal treatment. Methods: A total of 67 patients were initially screened in this study, and 60 patients were randomly allocated into 1 of the 3 groups, with 53 patients completing this study. At baseline, the average probing depth across all groups was 3.60 mm, with an average bleeding on probing (BoP) score of 69,15%. Clinical parameters were assessed at multiple time points over 12 weeks. Results: The plaque levels reduced significantly in all groups: MTB decreased from 65,57% to 33.26%, ETB from 64,17% to 31.49%, and ETBV from 63,04% to 26.25% (p < 0.001 for all groups). Furthermore, a significant improvement for BoP was recorded across all groups: BoP decreased to 25.68% (MTB), 22.32% (ETB), and 23.14% (ETBV) (p < 0.001 for all groups). However, no significant difference in the plaque removal efficacy was observed between manual and electric toothbrushes, irrespective of visual feedback (p > 0.190). A linear mixed model analysis showed a significant overall group effect on the periodontal health parameters (p = 0.041) but no interaction with time (p = 0.965). Conclusions: These findings emphasize the importance of consistent oral hygiene in reducing periodontal disease. Further research is necessary to explore effective strategies for enhancing patient adherence and optimizing health outcomes.
Repeatability of Pentacam HR in Keratoconus According to Two Different Scan Protocols: 25-3D Scan and 50-Cornea Fine
Authors: Romano Davide , Coco Giulia , Borgia Alfredo , Calza Stefano , Kaye Stephen , Gadhvi Kunal , Semeraro Francesco , Romano Vito
Published: January 2025

Background: This study aims to evaluate the repeatability of the Pentacam HR, comparing two different measurement modes (50-cornea fine and 25-3D scan) in patients affected by keratoconus. Methods: Multicenter retrospective study, conducted at Eye Clinic of the ASST-Spedali Civili-University of Brescia, Italy, and St. Paul’s Eye Unit, Royal Liverpool University Hospital, United Kingdom. A total of 72 eyes from 72 patients with keratoconus underwent six consecutive measurements, three using the 25-3D scan mode and three with the 50-Cornea fine mode. Measurements were made by one single observer, using the Scheimpflug corneal tomographer camera (Pentacam HR, Oculus, Wetzlar, Germany). Repeatability was assessed using the within-subject SD (Sw) statistic from a two-way analysis of variance. Results: Both measurement modes had excellent repeatability. The interclass coefficient correlation (ICC) was excellent (>0.9) in all the parameters evaluated, apart from anterior and posterior astigmatic axes and posterior astigmatism (ICC > 0.8) and index of height asymmetry (IHA) (ICC < 0.6). However, in 18 of 29 parameters, the ICC was higher in case of 25-3D scan. Repeatability limit for Kmax was 1.00D in 25-3D scan mode and 1.02D in case of 50-cornea fine. Conclusions: 25-3D scan may be preferable to 50-Cornea fine, in view of having slightly higher ICC in case of patients with keratoconus. Repeatability limits reported may be helpful in clinical practice for assessing the progression of keratoconus.
Three-Dimensional Planning for Vascularized Bone Grafts: Implementation and Surgical Application for Complex Bone Reconstruction in the Hand and Forearm
Authors: Zaussinger Maximilian , Schwaiger Karl , Schwarzbauer Jürgen , Bachleitner Kathrin , Holzbauer Matthias , Ehebruster Gudrun , Schmidt Manfred
Published: January 2025

Background/Objectives: Vascularized bone grafts have been successfully established for complex bone defects. The integration of three-dimensional (3D) simulation and printing technology may aid in more precise surgical planning and intraoperative bone shaping. The purpose of the present study was to describe the implementation and surgical application of this innovative technology for bone reconstruction. Methods: This prospective pilot study was conducted between June 2019 and June 2024. For this evaluation, patients who received vascularized bone reconstruction assisted with 3D technology were included. For reconstruction, the free medial femoral condyle (MFC) flap was used as the vascularized bone graft. Patient-specific 3D-printed templates, based on individual 3D simulations according to defect characteristics, were used for surgical planning, including flap elevation, shaping and inset. Results: A total of six patients (five male) with an average age of 39 years (range 19–62 years) and a mean follow-up time of 15 months (range 5–24 months) were analysed. The indications were as follows: avascular necrosis of the carpal bones, a metacarpal defect after tumor resection and pseudoarthrosis after a fractured ulna. Three patients received an osteochondral and three patients received a cortico-cancellous MFC flap. Conclusions: Our evaluation of clinical application revealed enhanced preoperative planning as well as intraoperative performance. Although the implementation for this technology is challenging, the new insights gained in planning and surgical guidance have led us to incorporate this technology into our standard routine.
An Investigation into the Pathomechanism and Prognostic Markers for Predicting the Outcome of Patients with Moderate and Severe Aortic Stenosis with or Without Intervention
Authors: Kardos Attila , Mittal Tarun
Published: January 2025

Our understanding of the impact of aortic valve stenosis (AS) on patient outcomes irrespective of its severity has changed since the advent of populational databases on AS following the landmark publication by Ross and Braunwald in 1968 [...]
Associations Between Lifestyle Factors, Oral Health Behaviors, and Glycemic Control in Type 2 Diabetic Patients
Authors: Bolchis Vanessa , Alexa Iulia , Toderas Nicoleta A. , Dumitrescu Ramona , Sava-Rosianu Ruxandra , Balean Octavia , Alexa Vlad Tiberiu , Popescu Simona , Jumanca Daniela , Galuscan Atena , Ilia Iosif , Chioran Doina
Published: January 2025

Introduction: T2DM mellitus (T2DM) is a major global health issue associated with significant morbidity, mortality, and economic burden. While the role of lifestyle factors in glycemic control is well-established, the influence of oral health behaviors remains underexplored. Objective: This study aimed to investigate the interplay between lifestyle habits, oral health behaviors, and glycemic control in patients with T2DM. Methodology: A cross-sectional study was conducted on 132 patients (66 men and 66 women) with T2DM at the Pius Brînzeu Emergency Hospital in Timișoara, Romania. Data on smoking, physical activity, alcohol consumption, tooth brushing frequency, and dental visits were collected using structured questionnaires, and glycemic control was assessed through HbA1c measurements. Statistical analyses, including Pearson correlations and linear regression, were performed. Results: Among men, HbA1c levels were negatively associated with exercise frequency (ß = −0.26, p < 0.05) and education level (correlation coefficient −0.27, p < 0.05), and positively associated with dental visits and tooth brushing frequency (correlation coefficient 0.26, p < 0.05). In the combined analysis, education level positively correlated with both dental visits (correlation coefficient 0.24, p < 0.01) and alcohol consumption (correlation coefficient 0.22, p < 0.05). Conclusions: These findings underscore the importance of integrating oral health and lifestyle interventions into diabetes management to optimize patient outcomes.
Maternal and Fetal Complications in Pregnant Women with Neurofibromatosis Type 1: Literature Review and Two Case Reports
Authors: Nastac Ancuta , Panaitescu Anca Maria , Huluță Iulia , Gică Nicolae , Gorecki Gabriel-Petre , Botezatu Radu , Tutunaru Cristina Violeta , Voiculescu Vlad Mihai , Nedelea Florina Mihaela
Published: January 2025

Neurofibromatosis is a genetic disorder arising de novo or with an autosomal dominant transmission that typically presents either at birth or in early childhood, manifesting through distinctive clinical features such as multiple café-au-lait spots, benign tumors in the skin, bone enlargement, and deformities. This literature review aims to resume the spectrum of maternal and fetal complications encountered in pregnant women with neurofibromatosis type 1 (NF1). Thorough research was conducted on databases such as Web of Science, PubMed, Science Direct, Google Scholar, and Wiley Online Library. This review includes 48 case reports, original studies, and reviews on NF1 in pregnancy. The research on the interlink between NF1 and fertility and its influence on human-assisted reproduction techniques is limited. Preimplantation testing (by in vitro fertilization) and prenatal diagnosis (by chorionic villus sampling or amniocentesis) are available to detect affected fetuses. However, genotype–phenotype correlation is difficult to predict. Preconceptional planning and targeted investigations are crucial in understanding the extent of maternal disease. Although in some cases lesions can evolve rapidly during pregnancy, most pregnancies and births in NF1 go well with careful planning. There is a higher incidence of pheochromocytomas and pre-eclampsia, vascular rupture, and cardio-respiratory issues. Anesthesia at birth is a challenge in most cases, and before offering spinal anesthesia, imaging tests should be performed to characterize spinal lesions. General anesthesia may also be challenging when the disease affects the face, neck, upper spine, or airways. Birth-related difficulties may arise because of large neurofibromas located at the level of skin incision or birth canal; uterine atony may be expected if there are uterine lesions. Some complications can develop in postpartum, and affected women should be carefully followed even after pregnancy. Fetal risks include preterm birth (spontaneous or iatrogenic), growth restriction and developmental issues, birth complications, cardiovascular risk, and fetal/neonatal demise. Pregnancies in women with NF1 should be regarded as high-risk and followed in a multidisciplinary fashion. Careful assessment of lesions is of utmost importance before and during pregnancy for anticipating potential maternal risks and before birth to plan anesthesia and delivery.
Development and Treatment of Radicular Cyst in Pediatric Patient—Case Report
Authors: Kuźniarski Amadeusz , Kiryk Jan , Kiryk Sylwia , Kijak Edward , Dubowik Magdalena Aleksandra , Matys Jacek , Dobrzyński Maciej
Published: January 2025

Background: Inflammation in the periapical area of primary teeth can affect the development and eruption of permanent teeth. In an asymptomatic course, they are detected accidentally during routine examinations. In such cases, they often reach significant dimensions and cause irreversible changes. Methods: This report presents the case of a 9-year-old boy with a radicular cyst in the mandible, resulting in the displacement of both premolar tooth buds. To address the inflammation, facilitate the proper eruption of the impacted teeth, and prevent adjacent teeth from shifting, the primary molars were extracted. Marsupialization was performed under general anesthesia, followed by the fabrication and placement of an obturator. Results: Two months after treatment, the displaced tooth buds began aligning along the natural eruption pathway. By the 8-month follow-up, the premolars had successfully erupted into their proper positions in the oral cavity. Conclusions: Marsupialization combined with the use of an obturator is an effective first-line treatment for radicular cysts in pediatric patients, offering a conservative approach that promotes natural tooth eruption while preserving the surrounding dentition.