bluuebunny/crossref_metadata_2025 huggingface dataset:
Ferrets (Mustela furo) Are Aware of Their Dimensions
Authors: Khvatov Ivan A. , Sokolov Alexey Yu. , Kharitonov Alexander N.
Published: January 2023

Self-awareness is a complex phenomenon expressed as the ability of an individual to separate “self-entity” from “other entity”. One of its earliest evolutionary components is body size awareness, namely, the ability to consider the boundaries of one’s own body as factors influencing interaction with surrounding objects. For ferrets, Mustela furo, the task requiring the penetration of various holes is ecologically relevant. We designed an experimental study in which the ferrets were supposed to select one opening out of three to get the bait. The first experiment was aimed at studying whether ferrets would prefer the holes basing on the hole size. In the second experiment, we tested the ferrets’ ability to select a single passable hole on the first try while the impassable ones were larger in area. Results from the first experiment show that when choosing from the three passable openings, the animals preferred the shortest path to the bait and ignored the size of the holes. In the second experiment, all tested ferrets preferred to penetrate the passable opening on the first attempt, even though the areas of the two impenetrable ones were larger. We argue that these data indicate that ferrets are aware of their own body size.
Transcriptome Analysis Reveals Effect of Dietary Probiotics on Immune Response Mechanism in Southern Catfish (Silurus meridionalis) in Response to Plesiomonas shigelloides
Authors: Wang Rongrong , Qian Jiaming , Ji Da , Liu Xingyu , Dong Ranran
Published: January 2023

To explore whether a probiotic complex composed of Lactobacillus rhamnosus, Lactobacillus plantarum, and Lactobacillus casei can prevent or inhibit the inflammatory response caused by the invasion of Plesiomonas shigelloides in the southern catfish, we screened differentially expressed genes and enriched inflammation-related pathways among a control and three experimental groups and conducted analysis by transcriptome sequencing after a 21-day breeding experiment. Compared with those in the PS (Plesiomonas shigelloides) group, southern catfish in the L-PS (Lactobacillus-Plesiomonas shigelloides) group had no obvious haemorrhages or ulcerations. The results also showed that inflammation-related genes, such as mmp9, cxcr4, nfkbia, socs3, il-8, pigr, tlr5, and tnfr1, were significantly upregulated in the PS group compared with those in the L-PS groups. In addition, we verified six DEGs (mmp9, cxcr4, nfkbia, socs3, rbp2, and calr) and three proteins (CXCR4, NFKBIA, and CALR) by qRT-PCR and ELISA, respectively. Our results were consistent with the transcriptome data. Moreover, significantly downregulated genes (p < 0.05) were enriched in inflammation-related GO terms (lymphocyte chemotaxis and positive regulation of inflammatory response) and immune-related pathways (intestinal immune network for IgA production and IL-17 signalling pathway) in the L-PS vs. the PS group. Our results indicate that the infection of P. shigelloides can produce an inflammatory response, and probiotics could inhibit the inflammatory response caused by P. shigelloides to some extent.
Farmers Views on the Implementation of On-Farm Emergency Slaughter for the Management of Acutely Injured Cattle in Ireland
Authors: McDermott Paul , McKevitt Aideen , Santos Flavia H. , Hanlon Alison J.
Published: January 2023

Four management options for acutely injured cattle in Ireland exist: treatment, unless cattle are severely injured; on-farm emergency slaughter (OFES); casualty slaughter (CS) if the animal is certified fit for transport; or euthanasia. OFES is designed to prevent transport of welfare-compromised cattle. An online survey of farmers in Ireland was carried out between April and July 2021 and focused on events during 2020. A theoretical framework of capacity, willingness, and opportunity was used to explore farmers’ perceptions. Responses from 94 farmers (49 dairy and 45 beef) were analysed; not all respondents answered all questions. Respondents indicated that the incidence of acutely injured cattle in Ireland is low. A majority reported not having an acutely injured animal for greater than 36 months. Most respondents had a positive attitude towards OFES for animal welfare reasons and were aware of relevant regulations and guidelines. Barriers to OFES included a lack of availability of OFES, and dairy farmers indicated that it had a similar financial impact as euthanasia. A parallel study with veterinarians indicated a higher incidence of acutely injured cattle in Ireland; the current results may be due to the demographic or the sensitivity of the topic. Nationwide electronic data capture on the cause of mortality could support improvements in the management of acutely injured cattle and enable surveillance of the proportion of these cattle undergoing OFES, euthanasia, or CS.
Effect of Detergents Based on Sodium Dodecyl Sulfate on Functional Metrics of Frozen–Thawed Collared Peccary (Pecari tajacu) Semen
Authors: Moreira Samara S. J. , Silva Andréia M. , Pereira Ana G. , Santos Romário P. , Dantas Maiko R. T. , Souza-Júnior João B. F. , Snoeck Paola P. N. , Silva Alexandre R.
Published: January 2023

We evaluated the effects of detergents based on sodium dodecyl sulfoxide (SDS) on the functional parameters of collared peccary frozen–thawed sperm. Semen aliquots from ten individuals were diluted in a Tris–egg yolk–glycerol extender alone or with 0.5% Equex STM® paste or SDS (at 0.1%, 0.3% or 0.5% (v/v) concentration). Samples were fast frozen in liquid nitrogen with a post-thaw evaluation of motility, membrane functionality and integrity, mitochondrial activity, sperm binding ability and thermal resistance. The treatments without SDS (41.8 ± 3.5%) and those containing Equex (41.8 ± 4.4%) or 0.1% SDS (41.2 ± 5.5%) provided greater sperm motility (p < 0.05) than those containing SDS 0.3% (30.5 ± 4.7%) and 0.5% (31.2 ± 6.3%). Immediately after thawing, only treatments containing 0.1% SDS effectively preserved sperm straightness (STR) when compared to the negative control. All treatments preserved the amplitude of lateral head (ALH) and straightness (STR) during a thermal resistance test (p > 0.05), but SDS 0.5% impaired the membrane functionality and mitochondrial activity after thawing (p < 0.05). All treatments provided a similar recovery of sperm binding ability after thawing (p < 0.05). Our results showed that the addition of 0.1% SDS to the Tris–yolk–glycerol extender optimized the freeze–thaw recovery of peccary semen.
Genetic Parameters of Growth Traits and Quantitative Genetic Metrics for Selection and Conservation of Mecheri Sheep of Tamil Nadu
Authors: Balasundaram Balakrishnan , Thiruvenkadan Aranganoor Kannan , Murali Nagarajan , Muralidharan Jaganadhan , Cauveri Doraiswamy , Peters Sunday Olusola
Published: January 2023

Determining the genetic and non-genetic sources of variation in a breed is vital for the formulation of strategies for its conservation and improvement. The present study was aimed at estimating the (co)variance components and genetic parameters of Mecheri sheep by fitting six different animal models in the restricted maximum likelihood method, with a preliminary investigation on the performance of animals for non-genetic sources of variation. A total of 2616 lambs were studied, and varying levels of significance were found for the effects of period, season, parity of dam, and birth type on different body-weight traits. Direct heritability estimates derived from the best animal model for body weight at birth, three months, six months, nine months, and twelve months were 0.21, 0.24, 0.10, 0.15, and 0.09, respectively, and the maternal heritability of the corresponding traits was 0.12, 0.05, 0.04, 0.04, and 0.04, respectively. The genetic correlations between the body-weight traits were all positive and moderate-to-strong, except for the correlation between birth weight and the other body-weight traits. The significance of non-genetic factors studied in this work demanded a correction to improve the accuracy of the direct selection of lambs for body-weight traits. The estimated genetic parameters identified the weaning weight as a selection criterion for the improvement in body weight of Mecheri lambs at different ages. Inbred individuals accounted for approximately 13% of the total population in the Mecheri sheep population studied. There were 877 founders in the population, and the actual effective population size was 128.48. The population’s mean generation interval was 3.26. The mean inbreeding values ranged from 0.005 to 0.010 across generations. The population’s average relatedness ranged from 0.001 to 0.014 across generations. Individual inbreeding was found to be 0.45 per cent for the entire population and 3.4 per cent for the inbred population.
Diet of Free Ranging American Mink (Neovison vison) in Denmark
Authors: Rørbæk René Worup , Andersen Tobias Astell , Pertoldi Cino , Jørgensen Alex , Pagh Sussie
Published: January 2023

Non-native American mink (Neovison vison) is a widely spread predator in Denmark. The feral population in mainland Denmark consists of captive-born mink that escaped from fur farms and wild mink born in nature, whereas the population on Bornholm is almost all wild-born mink. In this study, the diets of feral mink in mainland Denmark and on the island of Bornholm are analyzed. The aim of this study was to examine (1) whether the diet of the larger captive-born mink differs from that of the smaller wild-born mink, (2) assess the regional variations between the diets of mink in mainland Denmark and on Bornholm, and (3) investigate the seasonal variation in the diet composition of mink. The stomach contents of 364 mink (243 wild-born and 114 captive-born) culled in the years 2019–2022 were analyzed. Of these, 203 mink were from mainland Denmark, and 154 were from Bornholm. No significant differences were found between the diets of captive-born mink and wild-born mink or the mink found in mainland Denmark and on the island of Bornholm. Significantly more empty stomachs were found during spring than during the summer and autumn, suggesting a bottleneck in the diet during spring.
Effect of Air Exposure and Re-Submersion on the Histological Structure, Antioxidant Response, and Gene Expression of Procambarus Clarkii
Authors: Lei Xiangyu , Yang Lishi , Tan Liqi , Yang Qibin , Zhou Falin , Jiang Shigui , Huang Jianhua
Published: January 2023

Air exposure is an important environmental stressor during the transportation and cultivation of Procambarus clarkii. We evaluated the effect of re-submersion for 24 h after dry transportation for 24 h on the histological structure, antioxidant activity, and gene expression of crayfish. The antioxidant parameters of catalase (CAT), superoxide dismutase (SOD), malondialdehyde (MDA), and lactate dehydrogenase (LDH), and the relative expression of CAT, SOD, HSP70, and ferritin genes were subsequently measured in the hepatopancreas and gills at both stages. Histopathology found that air exposure led to vacuolation of the hepatopancreas and disorderly arrangement of respiratory epithelial cells (REC) in the gills. The activities of catalase (CAT), superoxide dismutase (SOD), malondialdehyde (MDA), and lactic dehydrogenase (LDH) in the hepatopancreas and gills increased with short-term air exposure. The relative expression of genes (CAT, SOD, HSP70, and Ferritin) were induced after short-term air exposure. During re-submersion, MDA content and CAT and SOD activities in the hepatopancreas and gills were restored after 24 h, however, LDH activity and hepatopancreatic tissue damage were not repaired. Our results indicate that air exposure can cause oxidative damage to P. clarkii, and CAT and SOD can be used to determine the response of crayfish exposed to air, in addition to some damage that can be eliminated after re-submersion to a limited degree. This study provides foundational data that re-submersion can improve crayfish performance under hypoxic stress to a certain extent and will lead to the development of more effective transportation strategies and decrease economic losses in the future.
Analytical and Clinical Evaluation of Two Methods for Measuring Erythrocyte Sedimentation Rate in Eastern Indigo Snakes (Drymarchon couperi)
Authors: Bogan James E.
Published: January 2023

Erythrocyte sedimentation rate (ESR) is a hematological test that can detect inflammatory activity within the body. Although not specific for any particular disease, ESR is often used as a screening “sickness indicator” due to its reliability and low cost. The Westergren method is a manual ESR technique commonly used but requires special graduated pipettes and over 1mL of whole blood, precluding its use in smaller patients where limited sample volumes can be obtained. A modified micro-ESR technique has been described using hematocrit capillary tubes but is used less commonly. ESR has been reported to be a useful inflammatory indicator in gopher tortoises (Gopherus polyphemus) and box turtles (Terrapene spp.) but not in Florida cottonmouth snakes (Agkistrodon conanti). Having an inexpensive screening test for inflammation can help guide medical decisions within conservation efforts of imperiled species. This study evaluated the correlation between these two ESR methodologies in threatened eastern indigo snakes (Drymarchon couperi, EIS) and found a very strong correlation (rs = 0.897), without constant or proportional biases and a reference interval of 0 (90% CI -1-1)–9 mm/h (90% CI 8-11) was defined. Additionally, a significant difference was found between healthy EIS and EIS in mid-ecdysis (p = 0.006) and EIS with gastric cryptosporidiosis (p = 0.006), indicating ESR as a useful inflammatory indicator in EIS.
Molecular Identification of Sarcocystis rileyi and Sarcocystis sp. (Closely Related to Sarcocystis wenzeli) in Intestines of Mustelids from Lithuania
Authors: Prakas Petras , Moskaliova Darija , Šneideris Donatas , Juozaitytė-Ngugu Evelina , Maziliauskaitė Evelina , Švažas Saulius , Butkauskas Dalius
Published: January 2023

The genus Sarcocystis is a group of numerous protozoan parasites having a two-host life cycle. Based on laboratory experiments and/or phylogenetic analysis results it was shown that seven Sarcocystis spp. producing sarcocsyts in bird tissues are transmitted via predatory placental mammals. To date the role of small mammals of the family Mustelidae in the distribution of avian Sarcocystis spp. have not been studied. During the current investigation, intestinal mucosa scrapings of 115 mustelids belonging to five species were tested for S. albifronsi, S. anasi, S. rileyi, and S. wenzeli infecting anseriforms and chickens. Microscopically, free sporocysts, sporulating oocysts, and loose oocysts were found in 61 samples (53.0%). Using nested PCR targeting the ITS1 region and sequencing, S. rileyi was confirmed in eight American minks, two European polecats and single European badger. Sarcocystis sp. was identified in one American mink and one European pine marten. Based on the partial ITS1 region this parasite showed that 100% identity to pathogenic Sarcocystis sp. caused a fatal infection in backyard chickens from Brazil. Phylogenetically, the Sarcocystis sp. identified in our study was most closely related to S. wenzeli parasitising domestic fowl (Gallus domesticus).
Supplementary Feeding of Grazing Inner Mongolian Cashmere Goats during Pregnancy—Based on “Nutrient Requirements of Cashmere Goats”
Authors: Wang Xin-Hui , Li Qing , Zheng Zi-Bin , Diao Xiao-Gao , He Li-Wen , Zhang Wei
Published: January 2023

This study aimed to conduct precise supplementation for pregnant cashmere goats under grazing based on the feeding standard. Eight Inner Mongolian pregnant cashmere goats of near-average body weight were selected at early gestation (44.41 ± 4.03 kg) and late gestation (46.54 ± 4.02 kg) to measure their nutrient intake. Then, two pregnant cashmere goat flocks, No. 10 (control group, on-farm supplement) and No. 11 (supplemented group, supplement based on standard), with the same goat herd structure and grassland type, were chosen to conduct the supplemental feeding experiment. The results showed that pregnant cashmere goats lacked daily the intake of dry matter, digestive energy, crude protein and most essential mineral elements under grazing. After supplemental feeding, the supplementation based on the feeding standard increased the cashmere length and cashmere length growth volume and decreased the cashmere fineness, with no statistical significance. The goat cashmere yield, goat weight after shearing, single and twin-birth kid weight and kids’ mature secondary hair follicle density were significantly higher in the supplemented group (p < 0.05). In conclusion, supplementation in accordance with “Nutrient Requirements of Cashmere Goats” can enhance pregnant cashmere goats’ fiber production, growth performance, fertility and kids’ secondary hair follicles development, which is of great importance for the healthy and precise nutrition and management of cashmere goats.
Fermented Chinese Herbal Medicine Promoted Growth Performance, Intestinal Health, and Regulated Bacterial Microbiota of Weaned Piglets
Authors: Chen Guang , Li Zhiqing , Liu Shuangli , Tang Tuo , Chen Qinghua , Yan Zhaoming , Peng Jie , Yang Zhikang , Zhang Guanfeng , Liu Yating , Zheng Mengli
Published: January 2023

To investigate the effects of fermented Chinese herbal medicine on growth performance, diarrhea rate, nutrient digestibility, and intestinal health of weaned piglets, and to provide the theoretical basis for applying fermented Chinese herbal medicines to weaned piglet production, a total of 162 weaned and castrated piglets at 25 days of age (Duroc × Landrace × Yorkshire, half male and half female) with an initial body weight of 7.77 ± 0.03 kg were randomly divided into the following three groups according to the principle of similar body weight: basal diet (CON) group, basal diet + 3 kg/t fermented Chinese herbal medicine (LFHM) group, and basal diet + 5 g/kg fermented Chinese herbal medicine (HFHM) group. Each group underwent six replicates and there were nine piglets in each replicate. The experiment lasted 24 days, i.e., 3 days for preliminary feeding, and 21 days for the experiment. From Day 1 of the experiment, the piglets were observed and recorded for diarrhea each day. As compared with the CON group, the results indicated: Following the addition of fermented Chinese herbal medicine, the piglets in the LFHM and HFHM groups increased final weight (FW); average daily feed intake (ADFI); average daily gain (ADG) (p < 0.01); apparent digestibility of crude protein (CP) (p < 0.05); as well as chymotrypsin, α-amylase, and lipase activities (p < 0.01). In addition, α-amylase activity in the LFHM group was higher than that in the HFHM group (p < 0.05); chymotrypsin activity in the LFHM group was lower than that in the HFHM group (p < 0.05); as compared with the CON group, the LFHM and the HFHM increased villus height (VH) and crypt depth (CD) in piglet jejunum; isovaleric acid concentration with the HFHM was higher than those with the CON and the LFHM (p < 0.05), but butyrate concentration with the HFFM was lower than those with the CON and the LFHM (p < 0.05). The high-throughput 16S rRNA sequencing of intestinal microbiota results showed that the LFHM and the HFHM affected the microbial α diversity index in weaned piglet colon (p < 0.01). In conclusion, fermented Chinese herbs can improve the growth performance of weaned piglets by promoting the secretion of intestinal digestive enzymes, changing intestinal microbial diversity, regulating the contents of intestinal short chain fatty acids (SCFAs), promoting intestinal health, and improving nutrients digestibility.
A Predator on the Doorstep: Kill Site Selection by a Lone Wolf in a Peri-Urban Park in a Mediterranean Area
Authors: Del Frate Marco , Bongi Paolo , Tanzillo Luigi , Russo Claudia , Benini Omar , Sieni Sara , Scandura Massimo , Apollonio Marco
Published: January 2023

The aim of the study was to assess which kill site characteristics were selected by a lone wolf living in a protected Mediterranean coastal area near the city of Pisa, Italy, where both wild and domestic ungulates were available as potential prey. Between 2017 and 2019, we monitored the wolf’s predatory behaviour through a combination of camera trapping and active search for kill sites and prey carcasses. The main prey found was the fallow deer (n = 82); only two wild boars and no domestic ungulates were found preyed upon. The features and habitat of kill sites were modelled to test for selection by the wolf. The habitat type of kill site was composed of meadows and pastures (89.3%), woods (7.3%), degraded coastal areas (1.9%), roads and rivers (1.1%), and marshes (0.5%). We calculated their distance from landscape features and ran a binomial generalised linear model to test the influence of such landscape variables. The distance of kill sites from landscape elements was significantly different from random control sites, and a positive selection for fences was found. In fact, the wolf pushed fallow deer towards a fence to constrain them and prevent them from escaping. We also analysed the body condition of predated fallow deer as a percentage of fat content in the bone marrow of the hind legs. Our results revealed the selection of the lone wolf for deer in good body condition. This is a possible outcome of the habitat selection shown by fallow deer in the study area, where fenced open pastures are the richest in trophic resources; therefore, our findings suggest a high efficacy for the lone wolf hunting strategy, but also the adoption of a high risk feeding strategy by deer. This study suggests that a lone predator can take advantage of human infrastructures to maximise its predatory effectiveness.
Social Support in a Novel Situation Aimed for Stunning and Euthanasia of Pigs May Be Increased by Familiar Pigs—A Behavioural Study with Weaners
Authors: Söderquist Astrid , Wallenbeck Anna , Lindahl Cecilia
Published: January 2023

The common method of stunning pigs using high concentration carbon dioxide prior to slaughter poses an animal welfare issue, as the gas is aversive. Proof of concept for using nitrogen gas encapsulated in high-expansion foam as an alternative non-aversive method for stunning pigs has recently been presented. However, the individually tested pigs showed distress-related responses to foam exposure, regardless of whether it was nitrogen- or air-filled. This study examined the effect of companionship from a familiar or unfamiliar pig on behaviours in 72 nine-weeks old pigs during exposure to air-filled foam. Escape attempts were observed by 75% of solitary pigs, 42% of pigs with unfamiliar conspecifics, and 33% of pigs with familiar conspecifics. Familiar pig pairs clearly preferred social contact during foam exposure, whereas this was not as clear in unfamiliar pig pairs, and their motivation for social contact could have multiple explanations. The results from this study highlight the importance of contact with conspecifics when studying animal welfare and suggest that familiarity between pigs is important for social support, thus emphasizing the importance of maintaining social groups to reduce distress in pigs at slaughter.
Identifying the Effects of Social Disruption through Translocation on African Elephants (Loxodonta africana), with Specifics on the Social and Ecological Impacts of Orphaning
Authors: Garaï Marion E. , Boult Victoria L. , Zitzer Heike R.
Published: January 2023

African elephants (Loxodonta africana) exhibit a long developmental period during which they acquire complex social and ecological knowledge through social networks. Central to this is that matriarchs and older individuals play an important role as repositories of information gained through experience. Anthropogenic interventions—including poaching, culling, translocation, and hunting—can disrupt elephants’ social networks, with implications for individual fitness and potential long-term population viability. Here, we draw on a unique long-running, individual-based dataset to examine the impacts of translocation on a population of elephants in South Africa, taking into consideration demographic rates, social dynamics, and ecological decision-making. Specifically, we compared two translocated groups: a group of unrelated culling Orphans and a family herd. We found that the Orphan group experienced accelerated reproductive rates when compared with the family herd. The Orphan group also fissioned more frequently and for longer periods of time, suggesting lower cohesiveness, and were less decisive in their large-scale movement decisions. These results add to the growing body of literature on the downstream impacts of social disruption for elephants. Whilst the translocation of culling Orphans is no longer practised in South Africa, we encourage careful consideration of any elephant translocation and the resulting social disruption.
Antimicrobial Chemotherapy for Recalcitrant Severe Human Periodontitis
Authors: Rams Thomas E. , Slots Jørgen
Published: January 2023

This study evaluated a combined systemic and topical anti-infective periodontal treatment of 35 adults who had experienced ongoing periodontal breakdown following conventional surgical periodontics. The prescribed anti-infective therapy, based on microbiological testing, consisted of a single course of metronidazole plus ciprofloxacin (23 patients), metronidazole plus amoxicillin/clavulanic acid (10 patients), and metronidazole plus ciprofloxacin followed by metronidazole plus amoxicillin/clavulanic acid (2 patients). In addition, the study patients received 0.1% povidone-iodine subgingival disinfection during non-surgical root debridement and daily patient administered oral irrigation with 0.1% sodium hypochlorite. At 1 and 5 years post-treatment, all study patients showed gains in clinical periodontal attachment with no further attachment loss, and significant decreases in pocket probing depth, bleeding on probing, and subgingival temperature. The greatest disease resolution occurred in patients who at baseline harbored predominantly major periodontal pathogens which post-antibiotics became non-detectable and substituted by non-periodontopathic viridans streptococci. The personalized and minimally invasive anti-infective treatment regimen described here controlled periodontitis disease activity and markedly improved the clinical and microbiological status of the refractory periodontitis patients.
How to Use Nebulized Antibiotics in Severe Respiratory Infections
Authors: Gorham Julie , Taccone Fabio S. , Hites Maya
Published: January 2023

Difficult-to-treat pulmonary infections caused by multidrug-resistant (MDR) pathogens are of great concern because their incidence continues to increase worldwide and they are associated with high morbidity and mortality. Nebulized antibiotics are increasingly being used in this context. The advantages of the administration of a nebulized antibiotic in respiratory tract infections due to MDR include the potential to deliver higher drug concentrations to the site of infection, thus minimizing the systemic adverse effects observed with the use of parenteral or oral antibiotic agents. However, there is an inconsistency between the large amount of experimental evidence supporting the administration of nebulized antibiotics and the paucity of clinical studies confirming the efficacy and safety of these drugs. In this narrative review, we describe the current evidence on the use of nebulized antibiotics for the treatment of severe respiratory infections.
Current Understanding of the Molecular Basis of Spices for the Development of Potential Antimicrobial Medicine
Authors: Khatri Purnima , Rani Asha , Hameed Saif , Chandra Subhash , Chang Chung-Ming , Pandey Ramendra Pati
Published: January 2023

Antimicrobial resistance increases day by day around the world. To overcome this situation new antimicrobial agents are needed. Spices such as clove, ginger, coriander, garlic, and turmeric have the potential to fight resistant microbes. Due to their therapeutic properties, medicinal herbs and spices have been utilized as herbal medicines since antiquity. They are important sources of organic antibacterial substances that are employed in treating infectious disorders caused by pathogens such as bacteria. The main focus of the study is the bioactivity of the active ingredients present in different kinds of naturally available spices. We conducted a thorough search of PubMed, Google Scholar, and Research Gate for this review. We have read many kinds of available literature, and in this paper, we conclude that many different kinds of naturally available spices perform some form of bioactivity. After reading several papers, we found that some spices have good antimicrobial and antifungal properties, which may help in controlling the emerging antimicrobial resistance and improving human health. Spices have many phytochemicals, which show good antimicrobial and antifungal effects. This review of the literature concludes that the natural bioactivate compounds present in spices can be used as a drug to overcome antimicrobial resistance in human beings.
Antimicrobial Effects of Tetraspanin CD9 Peptide against Microbiota Causing Armpit Malodour
Authors: Al-Talib Hassanain , Abdulwahab Marwa Hasan , Murad Khairiyah , Amiruddin Nur Deanna , Mohamed Normi Ngah
Published: January 2023

Synthetic peptides, including tetraspanin CD9 peptides, are increasingly coming into focus as new treatment strategies against various organisms, including bacteria, that cause underarm odour. The use of deodorants and antiperspirants is associated with side effects. Therefore, it is critical to find an alternative therapeutic approach to combat underarm odour. The aim of this study is to investigate the antibacterial effect of tetraspanin CD9 peptides against the skin microbiota that cause malodour in the underarms. The antimicrobial activity of CD9 peptides against Micrococcus luteus (M. luteus), Bacillus subtilis (B. subtilis), Staphylococcus epidermidis (S. epidermidis), and Corynebacterium xerosis (C. xerosis) was investigated by the disc diffusion method. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by broth microdilution assays using CD9 peptide concentrations ranging from 1 mg/mL to 0.0078 mg/mL. In addition, the anti-biofilm activity of the CD9 peptides was determined. The CD9 peptides showed different antibacterial activity with an inhibition zone of 7.67, 9.67, 7.00, and 6.00 mm for S. epidermidis, M. luteus, C. xerosis, and B. subtilis, respectively. All bacteria had the same MBC value of 1 mg/mL. A high MIC of CD9 peptides was observed for S. epidermidis and M. luteus at 0.5 mg/mL. The MIC values of B. subtilis and C. xerosis were 0.125 mg/mL and 0.25 mg/mL, respectively. CD9 peptides significantly inhibited biofilm development of S. epidermidis, B. subtilis, and C. xerosis isolates. The CD9 tetraspanin peptide has excellent antibacterial activity against bacteria that cause underarm odour. Therefore, the CD9 tetraspanin peptide is a promising alternative to deodorants and antiperspirants to combat commensal bacteria of the skin that cause underarm odour.
Adequacy of the 10 mg/kg Daily Dose of Antituberculosis Drug Isoniazid in Infants under 6 Months of Age
Authors: López-Ramos Maria Goretti , Vinent Joan , Aarnoutse Rob , Colbers Angela , Velasco-Arnaiz Eneritz , Martorell Loreto , Falcón-Neyra Lola , Neth Olaf , Prieto Luis , Guillén Sara , Baquero-Artigao Fernando , Méndez-Echevarría Ana , Gómez-Pastrana David , Jiménez Ana Belén , Lahoz Rebeca , Ramos-Amador José Tomás , Soriano-Arandes Antoni , Santiago Begoña , Farré Rosa , Fortuny Clàudia , Soy Dolors , Noguera-Julian Antoni
Published: January 2023

In 2010, the WHO recommended an increase in the daily doses of first-line anti-tuberculosis medicines in children. We aim to characterize the pharmacokinetics of the once-daily isoniazid (INH) dose at 10 mg/kg of body weight in infants <6 months of age. We performed a multicenter pharmacokinetic study in Spain. The N-acetyltransferase 2 gene was analyzed to determine the acetylation status. Samples were analyzed using a validated UPLC-UV assay. A non-compartmental pharmacokinetic analysis was performed. Twenty-three pharmacokinetic profiles were performed in 20 infants (8 females) at a median (IQR) age of 19.0 (12.6–23.3) weeks. The acetylator statuses were homozygous fast (n = 1), heterozygous intermediate (n = 12), and homozygous slow (n = 7). INH median (IQR) Cmax and AUC0–24h values were 4.8 (3.7–6.7) mg/L and 23.5 (13.4–36.7) h*mg/L and the adult targets (>3 mg/L and 11.6–26.3 h*mg/L) were not reached in three and five cases, respectively. The age at assessment or acetylator status had no impact on Cmax values, but a larger INH AUC0–24h (p = 0.025) and trends towards a longer half-life (p = 0.055) and slower clearance (p = 0.070) were observed in homozygous slow acetylators. Treatment was well tolerated; mildly elevated alanine aminotransferase levels were observed in three cases. In our series of young infants receiving isoniazid, no major safety concerns were raised, and the target adult levels were reached in most patients.
Antimicrobial Resistance and Recent Alternatives to Antibiotics for the Control of Bacterial Pathogens with an Emphasis on Foodborne Pathogens
Authors: Helmy Yosra A. , Taha-Abdelaziz Khaled , Hawwas Hanan Abd El-Halim , Ghosh Soumya , AlKafaas Samar Sami , Moawad Mohamed M. M. , Saied Essa M. , Kassem Issmat I. , Mawad Asmaa M. M.
Published: January 2023

Antimicrobial resistance (AMR) is one of the most important global public health problems. The imprudent use of antibiotics in humans and animals has resulted in the emergence of antibiotic-resistant bacteria. The dissemination of these strains and their resistant determinants could endanger antibiotic efficacy. Therefore, there is an urgent need to identify and develop novel strategies to combat antibiotic resistance. This review provides insights into the evolution and the mechanisms of AMR. Additionally, it discusses alternative approaches that might be used to control AMR, including probiotics, prebiotics, antimicrobial peptides, small molecules, organic acids, essential oils, bacteriophage, fecal transplants, and nanoparticles.
Inversion Theory Leveling as a New Methodological Approach to Antioxidant Thermodynamics: A Case Study on Phenol
Authors: Stamenković Nikola , Cerkovnik Janez , Poklar Ulrih Nataša
Published: January 2023

Antioxidants are various types of compounds that represent a link between biology and chemistry. With the development of theoretical and computational methods, antioxidants are now being studied theoretically. Here, a novel method is presented that aims to reduce the estimated wall times for DFT calculations that result in the same or higher degree of accuracy in the second derivatives over energy than is the case with the regular computational route (i.e., optimizing the reaction system at a lower model and then recalculating the energies at a higher level of theory) by applying the inversion of theory level to the universal chemical scavenger model, i.e., phenol. The resulting accuracy and wall time obtained with such a methodological setup strongly suggest that this methodology could be generally applied to antioxidant thermodynamics for some costly DFT methods with relative absolute deviation.
Overexpression of an Antioxidant Enzyme APX1 in cpr5 Mutant Restores its Pleiotropic Growth Phenotype
Authors: Qi Fan , Li Jianwei , Hong Xiufang , Jia Zhiyi , Wu Binyan , Lin Fucheng , Liang Yan
Published: January 2023

Breeding crops with enhanced immunity is an effective strategy to reduce yield loss caused by pathogens. The constitutive expresser of pathogenesis-related genes (cpr5) mutant shows enhanced pathogen resistance but retarded growth; thus, it restricts the application of cpr5 in breeding crops with disease resistance. Reactive oxygen species (ROS) play important roles in plant growth and defense. In this study, we determined that the cpr5 mutant exhibited excessive ROS accumulation. However, the mutation of respiratory burst oxidase homolog D (RBOHD), a reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase responsible for the production of ROS signaling in plant immunity, did not suppress excessive ROS levels in cpr5. Furthermore, the cpr5 mutant showed low levels of ascorbate peroxidase 1 (APX1), an important cytosolic ROS-scavenging enzyme. APX1 overexpression in the cpr5 background removed excessive ROS and restored the pleiotropic growth phenotype. Notably, APX1 overexpression did not reduce the resistance of cpr5 mutant to virulent strain Pseudomonas syringae pv. tomato (Pst) DC3000 and avirulent strain Pst DC3000 (avrRpt2). These results suggest that the removal of excessive ROS by APX1 overexpression restored the cpr5 growth phenotype while conserving pathogen resistance. Hence, our study provides a theoretical and empirical basis for utilizing CPR5 in the breeding of crops with disease resistance by effective oxidative stress management via APX1 expression.
Effects of UV Stress in Promoting Antioxidant Activities in Fungal Species Тrametes versicolor (L.) Lloyd and Flammulina velutipes (Curtis) Singer
Authors: Krsmanović Nenad , Rašeta Milena , Mišković Jovana , Bekvalac Kristina , Bogavac Mirjana , Karaman Maja , Isikhuemhen Omoanghe S.
Published: January 2023

Trametes versicolor and Flammulina velutipes, after submerged cultivation, with intermittent exposure to ultraviolet light (UV), were studied for Total Phenolic content (TP) and Total Proteins (TPR) contents and antioxidant properties against free radicals. The TP and TPR were determined by a spectrophotometric method and Lowry’s assay, respectively. Liquid chromatography with mass spectrometry (LC–MS/MS) was used to quantify polyphenols. Different in vitro assays determined the antioxidant activities of the extracts. Mycelia extract from F. velutipes after 14 days and filtrate from T. versicolor after 21 days of incubation gave the highest TP 59.60 ± 0.14 and 50.03 ± 0.66 mg GAE/g d.w., respectively. Mycelia extract from T. versicolor after 28 days of incubation had the highest TPR (183.53 ± 2.84 mg BSAE/g d.w.). The LC-MS/MS analysis indicated that p-hydroxybenzoic and protocatechuic acids are the most abundant. Trametes versicolor filtrate after 14 days and F. velutipes filtrate after 21 days (71.29 ± 0.54% and 73.5 ± 1.81, respectively) had the highest scavenging activity in SOA. Correlation analysis indicated that all extracts’ antioxidant (AO) potential strongly correlated with TP (R2 = 0.83–1.0). The data confirmed that stress factors such as UV exposure could stimulate the production of secondary metabolites and natural AOs, especially phenolic acids in test fungi.
Common Reactivity and Properties of Heme Peroxidases: A DFT Study of Their Origin
Authors: Ramos Daniel R. , Furtmüller Paul G. , Obinger Christian , Peña-Gallego Ángeles , Pérez-Juste Ignacio , Santaballa J. Arturo
Published: January 2023

Electronic structure calculations using the density-functional theory (DFT) have been performed to analyse the effect of water molecules and protonation on the heme group of peroxidases in different redox (ferric, ferrous, compounds I and II) and spin states. Shared geometries, spectroscopic properties at the Soret region, and the thermodynamics of peroxidases are discussed. B3LYP and M06-2X density functionals with different basis sets were employed on a common molecular model of the active site (Fe-centred porphine and proximal imidazole). Computed Gibbs free energies indicate that the corresponding aquo complexes are not thermodynamically stable, supporting the five-coordinate Fe(III) centre in native ferric peroxidases, with a water molecule located at a non-bonding distance. Protonation of the ferryl oxygen of compound II is discussed in terms of thermodynamics, Fe–O bond distances, and redox properties. It is demonstrated that this protonation is necessary to account for the experimental data, and computed Gibbs free energies reveal pKa values of compound II about 8.5–9.0. Computation indicates that the general oxidative properties of peroxidase intermediates, as well as their reactivity towards water and protons and Soret bands, are mainly controlled by the iron porphyrin and its proximal histidine ligand.
Berberis microphylla G. Forst Intake Reduces the Cardiovascular Disease Plasmatic Markers Associated with a High-Fat Diet in a Mice Model
Authors: Olivares-Caro Lia , Nova-Baza Daniela , Radojkovic Claudia , Bustamante Luis , Duran Daniel , Mennickent Daniela , Melin Victoria , Contreras David , Perez Andy J. , Mardones Claudia
Published: January 2023

Polyphenols are bioactive substances that participate in the prevention of chronic illnesses. High content has been described in Berberis microphylla G. Forst (calafate), a wild berry extensively distributed in Chilean–Argentine Patagonia. We evaluated its beneficial effect through the study of mouse plasma metabolome changes after chronic consumption of this fruit. Characterized calafate extract was administered in water, for four months, to a group of mice fed with a high-fat diet and compared with a control diet. Metabolome changes were studied using UHPLC-DAD-QTOF-based untargeted metabolomics. The study was complemented by the analysis of protein biomarkers determined using Luminex technology, and quantification of OH radicals by electron paramagnetic resonance spectroscopy. Thirteen features were identified with a maximum annotation level-A, revealing an increase in succinic acid, activation of tricarboxylic acid and reduction of carnitine accumulation. Changes in plasma biomarkers were related to inflammation and cardiovascular disease, with changes in thrombomodulin (−24%), adiponectin (+68%), sE-selectin (−34%), sICAM-1 (−24%) and proMMP-9 (−31%) levels. The production of OH radicals in plasma was reduced after calafate intake (−17%), especially for the group fed with a high-fat diet. These changes could be associated with protection against atherosclerosis due to calafate consumption, which is discussed from a holistic and integrative point of view.
Human Naa50 Shows Serotonin N-Acetyltransferase Activity, and Its Overexpression Enhances Melatonin Biosynthesis, Resulting in Osmotic Stress Tolerance in Rice
Authors: Lee Kyungjin , Back Kyoungwhan
Published: January 2023

A new clade of serotonin N-acetyltransferase (SNAT), the penultimate enzyme in the melatonin biosynthetic pathway, has been reported in the archaeon Thermoplasma volcanium. The closest homolog of archaea SNAT in human was an N-alpha-acetyltransferase50 (Naa50). To determine whether human Naa50 (hNaa50) shows SNAT enzyme activity, we chemically synthesized and expressed the hNaa50 gene in Escherichia coli, followed by Ni2+ affinity purification. Purified recombinant hNaa50 showed SNAT activity (Km and Vmax values of 986 μM and 1800 pmol/min/mg protein, respectively). To assess its in vivo function, hNaa50 was overexpressed in rice (hNaa50-OE). The transgenic rice plants produced more melatonin than nontransgenic wild-type rice, indicating that hNaa50 is functionally coupled with melatonin biosynthesis. Due to its overproduction of melatonin, hNaa50-OE had a higher tolerance against osmotic stress than the wild type. Enhanced expression of the chaperone genes BIP1 and CNX in hNaa50-OE plants was responsible for the increased tolerance. It is concluded that hNaa50 harbors serotonin N-acetyltransferase enzyme activity in addition to its initial N-alpha-acetyltransferase, suggesting the bifunctionality of the hNaa50 enzyme toward serotonin and protein substrates. Consequently, ectopic overexpression of hNaa50 in rice enhanced melatonin synthesis, indicating that hNaa50 is in fact involved in melatonin biosynthesis.
Development of a Collaborative Robotic Platform for Autonomous Auscultation
Authors: Lopes Daniel , Coelho Luís , Silva Manuel F.
Published: January 2023

Listening to internal body sounds, or auscultation, is one of the most popular diagnostic techniques in medicine. In addition to being simple, non-invasive, and low-cost, the information it offers, in real time, is essential for clinical decision-making. This process, usually done by a doctor in the presence of the patient, currently presents three challenges: procedure duration, participants’ safety, and the patient’s privacy. In this article we tackle these by proposing a new autonomous robotic auscultation system. With the patient prepared for the examination, a 3D computer vision sub-system is able to identify the auscultation points and translate them into spatial coordinates. The robotic arm is then responsible for taking the stethoscope surface into contact with the patient’s skin surface at the various auscultation points. The proposed solution was evaluated to perform a simulated pulmonary auscultation in six patients (with distinct height, weight, and skin color). The obtained results showed that the vision subsystem was able to correctly identify 100% of the auscultation points, with uncontrolled lighting conditions, and the positioning subsystem was able to accurately position the gripper on the corresponding positions on the human body. Patients reported no discomfort during auscultation using the described automated procedure.
Stable Artificial Autopolyploids of the Zn/Cd Accumulator Arabidopsis arenosa—A Promising Genetic Resource for Phytoremediation
Authors: Kurdziel Agnieszka , Sychta Klaudia , Sliwinska Elwira , Miszczak Szymon , Szarek-Łukaszewska Grażyna , Rostański Adam , Słomka Aneta
Published: January 2023

Arabidopsis arenosa is a good candidate for phytoremediation due to its high tolerance to Zn and Cd as well as its accumulation ability. However, its small size and low biomass are the largest obstacles to applying it on a broad scale. The aim was to obtain polyploid specimens, which tend to have higher biomass to increase the accumulation and translocation capacity of heavy metals in this metal-tolerant plant. Doubled polyploids (octaploids) were obtained via indirect organogenesis on a ½ MS medium supplemented with 1 mg L−1 TDZ, followed by rooting on the same medium without growth regulators. Callus tissue of a high endopolyploidy level (the (Ʃ>2C)/2C ratio over 2.5) obtained on seedling fragments on ½ MS supplemented with 2 mg L−1 2,4-D + 2 mg L−1 BAP served as a source material. Among the regenerants successfully obtained (without using antimitotic agents), over half, regardless of the stage of regenerant development, were octaploid (54–78%; 2C DNA = 1.642 pg). Octaploids were not affected by ploidy or in vitro culture conditions; they were fully fertile, produced normal pollen (~97% of viability), and set seeds capable of germinating (78%). Their cell and organ size was affected by genome doubling resulting in longer stomata, bigger pollen grains, and flowers with a larger area and width in comparison with tetraploid regenerants and initial plants. The promising results of measurements of morpho-anatomical, physiological, and reproductive parameters indicate that, in the future, after passing tolerance tests, the obtained polyploids could be used in phytoremediation of metal-contaminated areas.