bluuebunny/crossref_metadata_2025 huggingface dataset:
Nanoscale Topographical Effects on the Adsorption Behavior of Bone Morphogenetic Protein-2 on Graphite
Authors: Marquetti Izabele , Desai Salil
Published: February 2022

The interaction between bone morphogenetic protein-2 (BMP-2) and the surface of biomaterials is essential for the restoration of bone and cartilage tissue, inducing cellular differentiation and proliferation. The properties of the surface, including topology features, regulate the conformation and bioactivity of the protein. In this research, we investigated the influence of nanopatterned surfaces on the interaction of a homodimer BMP-2 with graphite material by combining molecular dynamics (MD) and steered molecular dynamics (SMD) simulations. The graphite substrates were patterned as flat, linear grating, square, and circular profiles in combination with BMP-2 conformation in the side-on configuration. Ramachandran plots for the wrist and knuckle epitopes indicated no steric hindrances and provided binding sites to type I and type II receptors. Results showed two optimal patterns that increased protein adsorption of the lower monomer while preserving the secondary structure and leaving the upper monomer free to interact with the cells. Charged residues arginine and lysine and polar residues histidine and tyrosine were the main residues responsible for the strong interaction with the graphite surface. This research provides new molecular-level insights to further understand the mechanisms underlying protein adsorption on nanoscale patterned substrates.
Role of Distinct Macrophage Populations in the Development of Heart Failure in Macrophage Activation Syndrome
Authors: Kuna Jakub , Żuber Zbigniew , Chmielewski Grzegorz , Gromadziński Leszek , Krajewska-Włodarczyk Magdalena
Published: February 2022

Macrophage activation syndrome (MAS) is one of the few entities in rheumatology with the potential to quickly cause multiple organ failure and loss of life, and as such, requires urgent clinical intervention. It has a broad symptomatology, depending on the organs it affects. One especially dangerous aspect of MAS’s course of illness is myocarditis leading to acute heart failure and possibly death. Research in recent years has proved that macrophages settled in different organs are not a homogenous group, with particular populations differing in both structure and function. Within the heart, we can determine two major groups, based on the presence of the C-C 2 chemokine receptor (CCR2): CCR2+ and CCR2−. There are a number of studies describing their function and the changes in the population makeup between normal conditions and different illnesses; however, to our knowledge, there has not been one touching on the matter of changes occurring in the populations of heart macrophages during MAS and their possible consequences. This review summarizes the most recent knowledge on heart macrophages, the influence of select cytokines (those particularly significant in the development of MAS) on their activity, and both the immediate and long-term consequences of changes in the makeup of specific macrophage populations—especially the loss of CCR2− cells that are responsible for regenerative processes, as well as the substitution of tissue macrophages by the highly proinflammatory CCR2+ macrophages originating from circulating monocytes. Understanding the significance of these processes may lead to new discoveries that could improve the therapeutic methods in the treatment of MAS.
Crosstalk between Melanin Concentrating Hormone and Endocrine Factors: Implications for Obesity
Authors: Prida Eva , Fernández-González Sara , Pena-León Verónica , Pérez-Lois Raquel , Fernø Johan , Seoane Luisa María , Quiñones Mar , Al Massadi Omar Al
Published: February 2022

Melanin-concentrating hormone (MCH) is a 19aa cyclic peptide exclusively expressed in the lateral hypothalamic area, which is an area of the brain involved in a large number of physiological functions and vital processes such as nutrient sensing, food intake, sleep-wake arousal, memory formation, and reproduction. However, the role of the lateral hypothalamic area in metabolic regulation stands out as the most relevant function. MCH regulates energy balance and glucose homeostasis by controlling food intake and peripheral lipid metabolism, energy expenditure, locomotor activity and brown adipose tissue thermogenesis. However, the MCH control of energy balance is a complex mechanism that involves the interaction of several neuroendocrine systems. The aim of the present work is to describe the current knowledge of the crosstalk of MCH with different endocrine factors. We also provide our view about the possible use of melanin-concentrating hormone receptor antagonists for the treatment of metabolic complications. In light of the data provided here and based on its actions and function, we believe that the MCH system emerges as an important target for the treatment of obesity and its comorbidities.
Proximity Profiling of the CFTR Interaction Landscape in Response to Orkambi
Authors: Iazzi Melissa , Astori Audrey , St-Germain Jonathan , Raught Brian , Gupta Gagan D.
Published: February 2022

Deletion of phenylalanine 508 (∆F508) of the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) anion channel protein is the leading cause of Cystic Fibrosis (CF). Here, we report the analysis of CFTR and ∆F508-CFTR interactomes using BioID (proximity-dependent biotin identification), a technique that can also detect transient associations. We identified 474 high-confidence CFTR proximity-interactors, 57 of which have been previously validated, with the remainder representing novel interaction space. The ∆F508 interactome, comprising 626 proximity-interactors was markedly different from its wild type counterpart, with numerous alterations in protein associations categorized in membrane trafficking and cellular stress functions. Furthermore, analysis of the ∆F508 interactome in cells treated with Orkambi identified several interactions that were altered as a result of this drug therapy. We examined two candidate CFTR proximity interactors, VAPB and NOS1AP, in functional assays designed to assess surface delivery and overall chloride efflux. VAPB depletion impacted both CFTR surface delivery and chloride efflux, whereas NOS1AP depletion only affected the latter. The wild type and ∆F508-CFTR interactomes represent rich datasets that could be further mined to reveal additional candidates for the functional rescue of ∆F508-CFTR.
The Daily Expression of ABCC4 at the BCSFB Affects the Transport of Its Substrate Methotrexate
Authors: Furtado André , Mineiro Rafael , Duarte Ana Catarina , Gonçalves Isabel , Santos Cecília R. , Quintela Telma
Published: February 2022

The choroid plexuses (CPs), located in the brain ventricles, form an interface between the blood and the cerebrospinal fluid named the blood-cerebrospinal barrier, which, by the presence of tight junctions, detoxification enzymes, and membrane transporters, limits the traffic of molecules into the central nervous system. It has already been shown that sex hormones regulate several CP functions, including the oscillations of its clock genes. However, it is less explored how the circadian rhythm regulates CP functions. This study aimed to evaluate the impact of sex hormones and circadian rhythms on the function of CP membrane transporters. The 24 h transcription profiles of the membrane transporters rAbca1, rAbcb1, rAbcc1, rAbcc4, rAbcg2, rAbcg4, and rOat3 were characterized in the CPs of intact male, intact female, sham-operated female, and gonadectomized rats. We found that rAbcc1 is expressed in a circadian way in the CPs of intact male rats, rAbcg2 in the CPs of intact female rats, and both rAbcc4 and rOat3 mRNA levels were expressed in a circadian way in the CPs of intact male and female rats. Next, using an in vitro model of the human blood–cerebrospinal fluid barrier, we also found that methotrexate (MTX) is transported in a circadian way across this barrier. The circadian pattern of Abcc4 found in the human CP epithelial papilloma cells might be partially responsible for MTX circadian transport across the basal membrane of CP epithelial cells.
Reynolds Sensitivity of the Wake Passing Effect on a LPT Cascade Using Spectral/hp Element Methods
Authors: Cassinelli Andrea , Mateo Gabín Andrés , Montomoli Francesco , Adami Paolo , Vázquez Díaz Raul , Sherwin Spencer J.
Published: February 2022

Reynolds-Averaged Navier–Stokes (RANS) methods continue to be the backbone of CFD-based design; however, the recent development of high-order unstructured solvers and meshing algorithms, combined with the lowering cost of HPC infrastructures, has the potential to allow for the introduction of high-fidelity simulations in the design loop, taking the role of a virtual wind tunnel. Extensive validation and verification is required over a broad design space. This is challenging for a number of reasons, including the range of operating conditions, the complexity of industrial geometries and their relative motion. A representative industrial low pressure turbine (LPT) cascade subject to wake passing interactions is analysed, adopting the incompressible Navier–Stokes solver implemented in the spectral/hp element framework Nektar++. The bar passing effect is modelled by leveraging a spectral-element/Fourier Smoothed Profile Method. The Reynolds sensitivity is analysed, focusing in detail on the dynamics of the separation bubble on the suction surface as well as the mean flow properties, wake profiles and loss estimations. The main findings are compared with experimental data, showing agreement in the prediction of wake traverses and losses across the entire range of flow regimes, the latter within 5% of the experimental measurements.
Design and Thermal Characterization of Two Construction Solutions with and without Incorporation of Macroencapsulated PCM
Authors: Figueiredo António , Rebelo Filipe , Samagaio António , Vicente Romeu , Lira Jorge
Published: February 2022

Improving the energy efficiency of new and existing building stock while fostering the use of renewable energy is one of the major goals of the Renovation Wave initiative promoted by the European Union. In this framework, the present research focuses on the design of an innovative and efficient construction solution for an external envelope and internal partitions that can improve energy efficiency and thermal comfort in lightweight construction technology for buildings. The use of phase change materials (PCMs), particularly in the macroencapsulated form, in building construction solutions or components enhances the buildings’ thermal mass without significantly increasing the solutions’ weight. Therefore, the solution herein developed is essentially targeted at lightweight building technology since the incorporation of a macroencapsulated PCM core will allow to store and release large amounts of energy per volume unit, in order to attenuate high indoor temperature fluctuations. In the scope of this study, the use of a thermally active core in a lightweight construction solution was designed and thermally characterized. Thus, an experimental campaign on the thermal properties of the solution containing macroencapsulated PCMs was performed, intended for applications in two twin full-scale cold-formed steel lightweight tiny houses. Regarding the hot box heat flux meter approach, the results revealed the following: good correlation between thermal conductivity and mean specimen temperatures for both construction assemblies tested, and significant thermal amplitude reduction with the use solution containing the macroencapsulated PCM core.
Further Evidence of Autosomal Recessive Inheritance of RPL3L Pathogenic Variants with Rapidly Progressive Neonatal Dilated Cardiomyopathy
Authors: Nannapaneni Hemanth , Ghaleb Stephanie , Arya Sandeep , Gajula Viswanath , Taylor Mary B. , Das Bibhuti B.
Published: February 2022

Neonatal dilated cardiomyopathy (DCM) is rare with high etiologic heterogeneity. Recently, biallelic, autosomal recessive, pathogenic variants in RPL3L (ribosomal protein L3-like) have been reported in the literature with severe early-onset DCM. In the present brief report, we identified two pathogenic RPL3L variants, each harbored in unaffected heterozygous parents: mother (RPL3L c.1076_1080delCCGTG (p.Ala359Glyfs*4)) and father (RPL3L c.80G > A (p.Gly27Asp)). Pathogenic variants were segregated as autosomal recessive to two offspring born with compound heterozygous RPL3L variants and affected by neonatal DCM. This is the second report in the literature to the best of our knowledge and our findings support the pathogenicity of biallelic RPL3L pathologic variants associated with rapidly progressive neonatal DCM and heart failure with a poor prognosis.
Considerations on Baseline Generation for Imaging AI Studies Illustrated on the CT-Based Prediction of Empyema and Outcome Assessment
Authors: Sexauer Raphael , Stieltjes Bram , Bremerich Jens , D’Antonoli Tugba Akinci , Schmidt Noemi
Published: February 2022

For AI-based classification tasks in computed tomography (CT), a reference standard for evaluating the clinical diagnostic accuracy of individual classes is essential. To enable the implementation of an AI tool in clinical practice, the raw data should be drawn from clinical routine data using state-of-the-art scanners, evaluated in a blinded manner and verified with a reference test. Three hundred and thirty-five consecutive CTs, performed between 1 January 2016 and 1 January 2021 with reported pleural effusion and pathology reports from thoracocentesis or biopsy within 7 days of the CT were retrospectively included. Two radiologists (4 and 10 PGY) blindly assessed the chest CTs for pleural CT features. If needed, consensus was achieved using an experienced radiologist’s opinion (29 PGY). In addition, diagnoses were extracted from written radiological reports. We analyzed these findings for a possible correlation with the following patient outcomes: mortality and median hospital stay. For AI prediction, we used an approach consisting of nnU-Net segmentation, PyRadiomics features and a random forest model. Specificity and sensitivity for CT-based detection of empyema (n = 81 of n = 335 patients) were 90.94 (95%-CI: 86.55–94.05) and 72.84 (95%-CI: 61.63–81.85%) in all effusions, with moderate to almost perfect interrater agreement for all pleural findings associated with empyema (Cohen’s kappa = 0.41–0.82). Highest accuracies were found for pleural enhancement or thickening with 87.02% and 81.49%, respectively. For empyema prediction, AI achieved a specificity and sensitivity of 74.41% (95% CI: 68.50–79.57) and 77.78% (95% CI: 66.91–85.96), respectively. Empyema was associated with a longer hospital stay (median = 20 versus 14 days), and findings consistent with pleural carcinomatosis impacted mortality.
Modelling the Effect of ‘Roller Dynamics’ on Storm Erosion: Sylt, North Sea
Authors: Dissanayake Pushpa , Brown Jennifer
Published: February 2022

Coastal storm erosion can lead to episodic morphological changes and hinterland flooding that requires sustainable management. An accurate estimation of storm erosion can determine the success of hazard mitigation strategies. Two morphological models, Delft3D and XBeach, were applied separately to a stormy period with “Roller” and “No Roller” wave dynamics activated, to estimate erosion of the beach and dune system on the Sylt island. This is the first numerical impact assessment of roller dynamics on coastal erosion using the two models. The choice of model had more impact on the hydrodynamic and morphological predictions than the option to include or omit roller dynamics. Agreement between measured and simulated waves was higher in Delft3D (R2 > 0.90 and RMSE < 0.15 m) than XBeach. Storm erosion in both models had the highest sensitivity to the roller parameter Beta. Both models predicted a similar storm erosion pattern along the coast, albeit different magnitudes. It is found that Delft3D cannot produce comparable storm erosion to XBeach, when the roller dynamics and avalanching are considered. Delft3D is less sensitive to the roller dynamics than XBeach. Including roller dynamics in Delft3D increased storm erosion up to 31% and in XBeach decreased the erosion down to 58% in the nearshore area, while the erosion in the dune area increased up to 13% in Deflt3D and up to 97% in XBeach. Both models are skilled in simulating storm impact. For the simulation of a storm period with intermittent calm periods, it is suggested that applying a time-varying parameter setting for wave dynamics and sediment transport to capture storm erosion and post-storm beach recovery processes could improve results. Such a modelling approach may ultimately increase the accuracy of estimating storm erosion to support coastal management activities (e.g., sand nourishment volume).
Diversity of Ophiostomatoid Fungi Associated with Dendroctonus armandi Infesting Pinus armandii in Western China
Authors: Wang Huimin , Wang Tiantian , Liu Ya , Zeng Fanyong , Zhang Haifeng , Decock Cony , Zhang Xingyao , Lu Quan
Published: February 2022

Pinus armandii (P. armandii) is extensively abundant in western China and, as a pioneer tree, and prominently influences local ecology. However, pine forests in this region have been significantly damaged by Dendroctonus armandi (D. armandi) infestations, in close association with ophiostomatoid fungi. This study aimed to identify the diversity of ophiostomatoid fungi associated with D. armandi infesting P. armandii in western China. A total of 695 ophiostomatoid fungal strains were isolated from 1040 tissue pieces from D. armandi galleries and 89 adult beetles at four sites. In this study, based on multiloci DNA sequence data, as well as morphological and physiological characteristics, seven species belonging to five genera were identified including three known species, Esteyea vermicola, Graphium pseudormiticum and L. wushanense, two novel taxa, Graphilbum parakesiyea and Ophiostoma shennongense, and an unidentified Ophiostoma sp. 1. A neotype of Leptographium qinlingense. Ophiostoma shennongense was the dominant taxon (78.99%) in the ophiostomatoid community. This study provides a valuable scientific theoretical basis for the occurrence and management of D. armandi in the future.
Preposition Stranding in Spanish–English Code-Switching
Authors: Koronkiewicz Bryan
Published: February 2022

This study tests the acceptability of preposition stranding in the intrasentential code-switching of US heritage speakers of Spanish. Because languages vary when extracting determiner phrases from prepositional phrases, known as preposition stranding or p-stranding, a contrast arises for Spanish–English bilinguals. English allows p-stranding, but in Spanish the preposition is traditionally pied-piped with the DP. Heritage speakers of Spanish, though, have shown variability, with child sequential bilinguals requiring said pied-piping, but simultaneous bilinguals allowing p-stranding in Spanish. Participants (n = 24) completed a written acceptability judgment task with a 7-point Likert scale. The task included code-switched sentences (n = 16) with p-stranding, switching from either English to Spanish or vice versa, with comparison monolingual equivalents for Spanish (n = 8) and English (n = 8) included as well. The results found that the simultaneous bilinguals accepted p-stranding in both languages, while also showing no restriction in either code-switching condition. Child sequential bilinguals, however, showed the expected monolingual distinction between Spanish and English, and p-stranding was only accepted with Spanish determiner phrases extracted from an English prepositional phrase (i.e., Spanish-to-English). These findings support the previously reported differentiation between simultaneous and child sequential bilinguals regarding p-stranding, while expanding it to code-switching.
Towards Replacing Titanium with Copper in the Bipolar Plates for Proton Exchange Membrane Water Electrolysis
Authors: Kellenberger Andrea , Vaszilcsin Nicolae , Duca Delia , Dan Mircea Laurentiu , Duteanu Narcis , Stiber Svenja , Morawietz Tobias , Biswas Indro , Ansar Syed Asif , Gazdzicki Pawel , Wirkert Florian J. , Roth Jeffrey , Rost Ulrich , Brodmann Michael , Gago Aldo Saul , Friedrich K. Andreas
Published: February 2022

For proton exchange membrane water electrolysis (PEMWE) to become competitive, the cost of stack components, such as bipolar plates (BPP), needs to be reduced. This can be achieved by using coated low-cost materials, such as copper as alternative to titanium. Herein we report on highly corrosion-resistant copper BPP coated with niobium. All investigated samples showed excellent corrosion resistance properties, with corrosion currents lower than 0.1 µA cm−2 in a simulated PEM electrolyzer environment at two different pH values. The physico-chemical properties of the Nb coatings are thoroughly characterized by scanning electron microscopy (SEM), electrochemical impedance spectroscopy (EIS), X-ray photoelectron spectroscopy (XPS), and atomic force microscopy (AFM). A 30 µm thick Nb coating fully protects the Cu against corrosion due to the formation of a passive oxide layer on its surface, predominantly composed of Nb2O5. The thickness of the passive oxide layer determined by both EIS and XPS is in the range of 10 nm. The results reported here demonstrate the effectiveness of Nb for protecting Cu against corrosion, opening the possibility to use it for the manufacturing of BPP for PEMWE. The latter was confirmed by its successful implementation in a single cell PEMWE based on hydraulic compression technology.
Design, Synthesis, and Biological Evaluation of New Azulene-Containing Chalcones
Authors: Bala Daniela , Jinga Luiza-Izabela , Popa Marcela , Hanganu Anamaria , Voicescu Mariana , Bleotu Coralia , Tarko Laszlo , Nica Simona
Published: February 2022

Azulene-containing chalcones have been synthesized via Claisen–Schmidt condensation reaction. Their chemical structure has been established by spectroscopic methods where the 1H-NMR spectra suggested that the title chalcones were geometrically pure and configured trans (J = 15 Hz). The influence of functional groups from azulene-containing chalcones on the biological activity of the 2-propen-1-one unit was investigated for the first time. This study presents optical and fluorescent investigations, QSAR studies, and biological activity of 10 novel compounds. These chalcones were evaluated for their antimicrobial activity against Gram-positive and Gram-negative bacteria. The results revealed that most of the synthesized compounds showed inhibition against Gram-negative microorganisms, independent of the substitution of azulene scaffold. Instead, all azulene-containing chalcones exhibited good antifungal activity against Candida parapsilosis, with MIC values ranging between 0.156 and 0.312 mg/mL. The most active compound was chalcone containing azulene moieties on both sides of the 2-propene-1-one bond, exhibiting good activity against both bacteria-type strains and good antifungal activity. This antifungal activity combined with low toxicity makes azulene-containing chalcones a new class of bioorganic compounds.
Shell-Isolated Nanoparticle-Enhanced Raman Spectroscopy for Probing Riboflavin on Graphene
Authors: Zdaniauskienė Agnė , Ignatjev Ilja , Charkova Tatjana , Talaikis Martynas , Lukša Algimantas , Šetkus Arūnas , Niaura Gediminas
Published: February 2022

Graphene research and technology development requires to reveal adsorption processes and understand how the defects change the physicochemical properties of the graphene-based systems. In this study, shell-isolated nanoparticle-enhanced Raman spectroscopy (SHINERS) and graphene-enhanced Raman spectroscopy (GERS) coupled with density functional theory (DFT) modeling were applied for probing the structure of riboflavin adsorbed on single-layer graphene substrate grown on copper. Intense and detailed vibrational signatures of the adsorbed riboflavin were revealed by SHINERS method. Based on DFT modeling and detected downshift of prominent riboflavin band at 1349 cm−1 comparing with the solution Raman spectrum, π-stacking interaction between the adsorbate and graphene was confirmed. Different spectral patterns from graphene-riboflavin surface were revealed by SHINERS and GERS techniques. Contrary to GERS method, SHINERS spectra revealed not only ring stretching bands but also vibrational features associated with ribityl group of riboflavin and D-band of graphene. Based on DFT modeling it was suggested that activation of D-band took place due to riboflavin induced tilt and distortion of graphene plane. The ability to explore local perturbations by the SHINERS method was highlighted. We demonstrated that SHINERS spectroscopy has a great potential to probe adsorbed molecules at graphene.
An Integrated Approach to Using Sheep Wool as a Fibrous Material for Enhancing Strength and Transport Properties of Concrete Composites
Authors: Alyousef Rayed , Mohammadhosseini Hossein , Ebid Ahmed Abdel Khalek , Alabduljabbar Hisham
Published: February 2022

An important goal to achieve sustainable development is to use raw materials that are easily recyclable and renewable, locally available, and eco-friendly. Sheep wool, composed of 60% animal protein fibers, 10% fat, 15% moisture, 10% sheep sweat, and 5% contaminants on average, is an easily recyclable, easily renewable, and environmentally friendly source of raw material. In this study, slump testing, compressive and flexural strengths, ultrasonic pulse velocity, sorptivity, and chloride penetration tests were investigated to assess the influence of wool fibers on the strength and transport properties of concrete composites. Ordinary Portland cement was used to make five concrete mixes incorporating conventional wool fibers (WFs) ranging from 0.5 to 2.5% and a length of 70 mm. The wool fibers were modified (MWFs) via a pre-treatment technique, resulting in five different concrete compositions with the same fiber content. The addition of WF and MWF to fresh concrete mixes resulted in a decrease in slump values. The compressive strength of concrete was reduced when wool fibers were added to the mix. The MWF mixes, however, achieved compressive strength values of more than 30 MPa after a 90-day curing period. Furthermore, by including both WF and MWF, the flexural strength was higher than that of plain concrete. In addition, adding fibers with volume fractions of up to 2% reduced the concrete composite’s sorptivity rate and chloride penetration depths for both WF and MWF content mixes. Consequently, biomass waste like sheep wool could be recycled and returned to the field following the circular economy and waste valorization principles.
Some Aspects of Oxidation and Reduction Processes in Ti–Al and Ti–Al–Nb Systems
Authors: Mitoraj-Królikowska Marzena , Drożdż Ewa
Published: February 2022

The oxidation of titanium and titanium aluminides has attracted the attention of scientists for many years because of their high-temperature application. The most popular method to investigate oxidation behavior is the measurement of alloy mass changes during exposure to elevated-temperature under isothermal or thermal cycling conditions. However, the thermogravimetric method is not enough to establish an oxidation mechanism. In this paper, the temperature-programmed oxidation (TPOx) and reduction (TPR) were applied for the Ti–Al and Ti–Al–Nb systems, which was a new experimental concept which revealed interesting phenomena. Although oxidation of titanium alloys is well-described in the literature, not many papers present at the same time reduction of oxidized alloys. The results presented in the paper concentrated on the first stages of oxidation, which are scarcely described in the literature, but are important to understand the oxidation mechanism. Comparison between powder and bulk samples with similar compositions revealed essential differences in the oxidation mechanism.
Nanowall Textured Hydrophobic Surfaces and Liquid Droplet Impact
Authors: Yilbas Bekir Sami , Abubakar Abba , Yakubu Mubarak , Al-Qahtani Hussain , Al-Sharafi Abdullah
Published: February 2022

Water droplet impact on nanowires/nanowalls’ textured hydrophobic silicon surfaces was examined by assessing the influence of texture on the droplet impact dynamics. Silicon wafer surfaces were treated, resulting in closely packed nanowire/nanowall textures with an average spacing and height of 130 nm and 10.45 μm, respectively. The top surfaces of the nanowires/nanowalls were hydrophobized through the deposition of functionalized silica nanoparticles, resulting in a droplet contact angle of 158° ± 2° with a hysteresis of 4° ± 1°. A high-speed camera was utilized to monitor the impacting droplets on hydrophobized nanowires/nanowalls’ textured surfaces. The nanowires/nanowalls texturing of the surface enhances the pinning of the droplet on the impacted surface and lowers the droplet spreading. The maximum spreading diameter of the impacting droplet on the hydrophobized nanowires/nanowalls surfaces becomes smaller than that of the hydrophobized as-received silicon, hydrophobized graphite, micro-grooved, and nano-springs surfaces. Penetration of the impacted droplet fluid into the nanowall-cell structures increases trapped air pressure in the cells, acting as an air cushion at the interface of the droplet fluid and nanowalls’ top surface. This lowers the droplet pinning and reduces the work of droplet volume deformation while enhancing the droplet rebound height.
Enhancing Stability and Mucoadhesive Properties of Chitosan Nanoparticles by Surface Modification with Sodium Alginate and Polyethylene Glycol for Potential Oral Mucosa Vaccine Delivery
Authors: Amin Muhammad Khairul , Boateng Joshua Siaw
Published: February 2022

Background: The present study aimed to fabricate surface-modified chitosan nanoparticles with two mucoadhesive polymers (sodium alginate and polyethylene glycol) to optimize their protein encapsulation efficiency, improve their mucoadhesion properties, and increase their stability in biological fluids. Method: Ionotropic gelation was employed to formulate chitosan nanoparticles and surface modification was performed at five different concentrations (0.05, 0.1, 0.2, 0.3, 0.4% w/v) of sodium alginate (ALG) and polyethylene glycol (PEG), with ovalbumin (OVA) used as a model protein antigen. The functional characteristics were examined by dynamic light scattering (DLS), X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), and scanning electron microscopy (SEM)/scanning transmission electron microscopy (STEM). Stability was examined in the presence of simulated gastric and intestinal fluids, while mucoadhesive properties were evaluated by in vitro mucin binding and ex vivo adhesion on pig oral mucosa tissue. The impact of the formulation and dissolution process on the OVA structure was investigated by sodium dodecyl-polyacrylamide gel electrophoresis (SDS-PAGE) and circular dichroism (CD). Results: The nanoparticles showed a uniform spherical morphology with a maximum protein encapsulation efficiency of 81%, size after OVA loading of between 200 and 400 nm and zeta potential from 10 to 29 mV. An in vitro drug release study suggested successful nanoparticle surface modification by ALG and PEG, showing gastric fluid stability (4 h) and a 96 h sustained OVA release in intestinal fluid, with the nanoparticles maintaining their conformational stability (SDS-PAGE and CD analyses) after release in the intestinal fluid. An in vitro mucin binding study indicated a significant increase in mucin binding from 41 to 63% in ALG-modified nanoparticles and a 27–49% increase in PEG-modified nanoparticles. The ex vivo mucoadhesion showed that the powdered particles adhered to the pig oral mucosa. Conclusion: The ALG and PEG surface modification of chitosan nanoparticles improved the particle stability in both simulated gastric and intestinal fluids and improved the mucoadhesive properties, therefore constituting a potential nanocarrier platform for mucosal protein vaccine delivery.
Extremely Rare Case of Fetal Anemia Due to Mitochondrial Disease Managed with Intrauterine Transfusion
Authors: Chung Jinha , Lee Mi-Young , Chung Jin-Hoon , Won Hye-Sung
Published: February 2022

This report describes a rare case of fetal anemia, confirmed as a mitochondrial disease after birth, treated with intrauterine transfusion (IUT). Although mitochondrial diseases have been described in newborns, research on their prenatal features is lacking. A patient was referred to our institution at 32 gestational weeks owing to fetal hydrops. Fetal anemia was confirmed by cordocentesis. After IUT had been performed three times, the anemia and associated fetal hydrops showed improvement. However, after birth, the neonate had recurrent pancytopenia and lactic acidosis. He was eventually diagnosed with Pearson syndrome and died 2 months after birth. This is the first case report of fetal anemia associated with mitochondrial disease managed with IUT.
A Descriptive Analysis of Urinary ESBL-Producing-Escherichia coli in Cerdanya Hospital
Authors: Gaviria Lorena Patrícia , Montsant Lourdes , Azuaje Carlos , González-Díaz Aida , Horcajada Juan P. , Limón Enric , Viñas Miguel , Espinal Paula , Fusté Ester
Published: February 2022

Urinary tract infections caused by extended-spectrum β-lactamase Escherichia coli (ESBL-EC) are increasing worldwide and are a current concern because treatment options are often limited. This study investigated antimicrobial susceptibility, antimicrobial resistance genes (ARGs), and the biological diversity of urinary ESBL-EC isolates at Cerdanya Hospital, a European cross-border hospital that combines French and Spanish healthcare models. Bacterial identification and susceptibility were determined using the Microscan WalkAway® system and ESBL production was examined by the double-disk synergy method. Isolates were sequenced using the Ion S5™ next-generation sequencing system, with the whole-genome sequences then assembled using SPADEs software and analyzed using PubMLST, ResFinder, FimTyper, PlasmidFinder, and VirulenceFinder. A phylogenetic analysis was performed by constructing an assembly-based core-SNV alignment, followed by a phylogenetic tree constructed using Parsnp from the Harvest suite. All isolates studied were multidrug-resistant and could be classified into 19 different sequence types characterized by a high genetic diversity. The most prevalent ESBL-enzymes were CTX-M-14 and CTX-M-15. High-risk international clones (ST131, ST10, and ST405) were also identified. The results demonstrated the absence of a single predominant clone of ESBL-MDR-EC at Cerdanya Hospital.
Metabolically Healthy and Unhealthy Obese Phenotypes among Arabs and South Asians: Prevalence and Relationship with Cardiometabolic Indicators
Authors: Oguoma Victor M. , Abu-Farha Mohamed , Coffee Neil T. , Alsharrah Saad , Al-Refaei Faisal H. , Abubaker Jehad , Daniel Mark , Al-Mulla Fahd
Published: February 2022

Obesity is a public health crisis in Kuwait. However, not all obese individuals are metabolically unhealthy (MuHO) given the link between obesity and future cardiovascular events. We assessed the prevalence of the metabolically healthy obese (MHO) phenotype and its relationship with high sensitivity C-reactive protein (hs-CRP), serum alanine aminotransferase (ALT), and insulin resistance (HOMA-IR) in Arab and South Asian ethnic groups in Kuwait. The national cross-sectional survey of diabetes and obesity in Kuwait adults aged 18–60 years were analysed. The harmonised definition of metabolic syndrome was used to classify metabolic health. Multinomial logistic regression analysis was used to model the relationship between the MHO and MuHO phenotypes and hs-CRP, ALT and HOMA-IR levels. Overall, the prevalence of MHO for body mass index (BMI)- and waist circumference (WC)-defined obesity was 30.8% and 56.0%, respectively; it was greater in women (60.4% and 61.8%, respectively) than men (39.6% and 38.2%, respectively). Prevalence rates were also lower for South Asians than for Arabs. The MHO phenotype had hs-CRP values above 3 µg/mL for each age group category. Men compared to women, and South Asians compared to Arabs had a lower relative risk for the MHO group relative to the MuHO group. This study shows there is high prevalence of MHO in Kuwait.
Chronic Intermittent Sucrose Consumption Facilitates the Ability to Discriminate Opioid Receptor Blockade with Naltrexone in Rats
Authors: Jewett David C. , Liyanagamage Donisha S. N. K. , Avond Mark A. Vanden , Anderson Molly A. B. , Twaroski Kyleigh A. , Marek Morgan A. , James Kimberly F. , Pal Tapasya , Klockars Anica , Olszewski Pawel K. , Levine Allen S.
Published: February 2022

The opioid antagonist naltrexone (NTX) decreases intake of preferred diets in rats at very low doses relative to doses needed to decrease intake of “bland” laboratory chow. In the absence of an opioid agonist, NTX is not discriminable using operant techniques. In the current study, we found that rats given intermittent access to a 25% sucrose solution learned to discriminate between various naltrexone doses and saline. None of the rats given only water learned to discriminate between naltrexone and saline. When access to the sucrose solution was discontinued for 14 days, the rats lost the ability to discriminate between NTX and saline. We also studied the changes of c-Fos IR in selected brain regions in rats treated with saline versus NTX that were drinking water or 25% sucrose. An injection of NTX or saline resulted in a significant drug, diet, and interaction effect in various brain regions associated with feeding behavior, particularly the amygdala, accumbens, and hypothalamic sites. Thus, we found that ingestion of a sucrose solution results in the ability of rats to reliably discriminate naltrexone administration. In addition, sucrose and naltrexone altered c-Fos IR in an interactive fashion in brain regions known to be involved in ingestion behavior.
Anthropometric Measures and Risk of Rheumatoid Arthritis in the French E3N Cohort Study
Authors: Salliot Carine , Nguyen Yann , Mariette Xavier , Boutron-Ruault Marie-Christine , Seror Raphaèle
Published: February 2022

We aimed to assess the relationships between anthropometric measures and risk of rheumatoid arthritis (RA). The E3N cohort included 98,995 women (aged 40–65 years at the recruitment) who completed mailed questionnaires on reproductive factors, lifestyle, and health-related information, including anthropometric measures, every 2–3 years. Cox proportional hazards regression models with age as the time scale and adjusted on known RA risk factors were used to estimate hazard ratios (HRs) and 95% confidence intervals for the risk of incident RA in the overall population (n = 78,452) and after stratification on smoking exposure. Incident RA diagnosis was validated in 698 women. Abdominal obesity (waist circumference >88 cm) was associated with RA (HR = 1.2 (1.0–1.5)), independent of BMI; whereas obesity, defined as BMI ≥ 30 kg/m2, was marginally associated with RA (HR = 1.26 (0.9–1.5), ptrend = 0.0559). Taking lean body shape (BS) as reference, medium BS at puberty (HR = 1.3 (1.0–1.7)) and medium-large BS at perimenopausal period (HR = 1.5 (1.1–1.9)) were associated with the risk of RA among never-smoker women, independent of BMI. Regarding BS trajectory, taking constantly lean BS as reference, constantly large BS from puberty to perimenopause was associated with RA among non-smokers (HR = 2.10 (1.2–3.6)), independent of BMI.
Feasibility and Effectiveness of a Preventive Care Program during the Compound Humanitarian Crisis and COVID-19 Pandemic in Venezuela
Authors: González-Rivas Juan P. , Infante-García María M. , Nieto-Martinez Ramfis , Mechanick Jeffrey I. , Danaei Goodarz
Published: February 2022

Effective preventive care programs are urgently needed during humanitarian crises, as has been especially obvious during the COVID-19 pandemic. A pragmatic trial was designed: hybridized intervention (Diabetes Prevention Program [DPP] + medical nutrition therapy + liquid diet [LD]; LD group) vs. DPP only (DPP group). The participants were adults who were overweight/obese and at high risk of type 2 diabetes mellitus (T2DM). The LD consisted of a “homemade” milk- and fruit-juice-based beverage. Pandemic restrictions delayed the program by nine months, tripled the amount of time required for screening, and reduced the total sample to 60%. Eventually, 127 participants were randomized, and 94/127 participants (74.0%) completed the first phase. Participant dropout was influenced by migration, COVID-19 symptoms, education level, and socioeconomic status. In two months, the LD group lost 2.9 kg (p < 0.001) and the DPP group, 2.2 kg (p < 0.001) (between-group p = 0.170), with improvements in their cardiometabolic risk factors. At this stage, the DPP was shown to be feasible and effective, demonstrating weight loss with the improvement of cardiometabolic risk factors in a primary setting in Venezuela, a middle-income country with a chronic humanitarian crisis, during the COVID-19 pandemic.
Therapeutic Outcomes of Isatin and Its Derivatives against Multiple Diseases: Recent Developments in Drug Discovery
Authors: Cheke Rameshwar S. , Patil Vaishali M. , Firke Sandip D. , Ambhore Jaya P. , Ansari Iqrar A. , Patel Harun M. , Shinde Sachin D. , Pasupuleti Visweswara Rao , Hassan Md Imtaiyaz , Adnan Mohd , Kadri Adel , Snoussi Mejdi
Published: February 2022

Isatin (1H indole 2, 3-dione) is a heterocyclic, endogenous lead molecule recognized in humans and different plants. The isatin nucleus and its derivatives are owed the attention of researchers due to their diverse pharmacological activities such as anticancer, anti-TB, antifungal, antimicrobial, antioxidant, anti-inflammatory, anticonvulsant, anti-HIV, and so on. Many research chemists take advantage of the gentle structure of isatins, such as NH at position 1 and carbonyl functions at positions 2 and 3, for designing biologically active analogues via different approaches. Literature surveys based on reported preclinical, clinical, and patented details confirm the multitarget profile of isatin analogues and thus their importance in the field of medicinal chemistry as a potent chemotherapeutic agent. This review represents the recent development of isatin analogues possessing potential pharmacological action in the years 2016–2020. The structure–activity relationship is also discussed to provide a pharmacophoric pattern that may contribute in the future to the design and synthesis of potent and less toxic therapeutics.
Effect of Aging Time on the Composition of the Volatile Components of An–tea
Authors: Yao Ting , Wang Siqiang , She Xinsong , Zhou Di , Gan Zhuoting
Published: February 2022

To investigate the effects of different aging times, three An–tea samples were selected for comparison with a non-aged sample as the control (CK, one-year-old) and labeled as high-aged (HAS, 12 years old), medium-aged (MAS, 7 years old), and low-aged samples (LAS, 2 years old). Changes in the volatile components of the different An–tea samples were investigated using headspace–solid-phase microextraction (HS-SPME) combined with gas chromatography–mass spectrometry (GC-MS). The volatile components of An–tea consisted of eight types of compounds. Aldehydes and hydrocarbons were dominant in LAS, while esters, ketones, alcohols, nitrogen-containing compounds, and ethers were the most abundant compound in MAS. Esters were dominant in HAS, while phenols were only present in LAS and CK. As aging time increased, the number of identical compounds gradually decreased, while the relative contents of the alcohols also decreased. Except for CK, the contents of ketones and aldehydes gradually decreased as the aging time increased. The content of aldehydes greatly increased during the initial aging period. Ketones and esters had markedly increased in HAS, while their abundance in LAS was close to that in CK. The comprehensive quality score of the volatile components obtained by principal component analysis was highest for MAS, followed by HAS and LAS, with CK having the lowest score.
Beyond the Veil of Form: Developing a Transformative Approach toward Islamic Sacred Architecture through Designing a Contemporary Sufi Centre
Authors: Nasser Nevine
Published: February 2022

By examining the relationship between sacred space and spiritual experience through practice-as-research, a methodology for reclaiming the wisdom embodied by transformative examples of classic Islamic sacred architecture in the design of a contemporary Sufi Centre in London, UK, is developed. The metaphysical and ontological roots of universal design principles and practices are explored in order to transcend mimetic processes and notions of typology, location, time, style and scale in the creation of context-sensitive meanings and manifestations. An ontological hermeneutic approach was followed that utilises mixed methods underpinned by direct engagement, collaboration and a willingness to examine personal transcendent experiences and spiritual practices. By conducting practice, the effects of prioritising unseen dimensions (bātin), which enfold visible dimensions (zāhir), on understanding and designing Islamic sacred space are examined. The role of the imaginal realm, the imagination (khayāl), the spiritual heart (qalb) and spiritual inter-pretation (ta’wīl) are explored. Through a contemplative process, forms are perceived as conduits between the physical and spiritual realms and space as a symbol of presence (wujūd). Seen and unseen (zāhir wa bātin) converge into one continuum, potentiating an experience of Oneness (Tawhīd). A transformative approach to practice emerges that integrates a designers’ creative and spiritual practices, cultivates the capacity for transformation and helps to mitigate some of the challenges faced when designing sacred spaces in conventional settings today.
A Motion Compensation Method for Shipborne HFSWR by Using Dual Reference RF Signals Generated Onshore
Authors: Chen Maorong , Niu Jiong , Li Ming , Zhang Ling , Ji Yonggang , Wan Wenxiang , Wu Q. M. Jonathan
Published: February 2022

The echo of shipborne high-frequency surface wave radar (HFSWR) is modulated by six-degrees-of-freedom (6-DOF) motion, affecting the detection of the target and the remote sensing of ocean surface dynamics parameters. Commonly, motion compensation methods of shipborne HFSWR describe each aspect of the 6-DOF motion as the superposition of sinusoidal motion, which results in the effect of motion compensation affected by the precision of 6-DOF motion parameters. A motion compensation method based on dual reference radio frequency (RF) signals is proposed in this paper, without depending on a sinusoidal motion model to describe the 6-DOF motion. By using the motion compensation parameters, which are relevant to the motion attitude and calculated from the information of dual reference RF signals located onshore, the method realizes the compensation of shipborne HFSWR echo modulated by platform 6-DOF motion. This paper proposes the extraction of reference RF signals from radar echo and analyzes the influence of the location of the reference RF signals’ emission source on the motion compensation method. The result shows that a good motion compensation effect is achieved in eliminating the influence of 6-DOF motion modulation. In addition, a traversal of different reference RF signals’ emission source locations is conducted, and the simulation results show that the method proposed in this paper has universality.