bluuebunny/crossref_metadata_2025 huggingface dataset:
Dual-Channel Stopped-Flow Apparatus for Simultaneous Fluorescence, Anisotropy, and FRET Kinetic Data Acquisition for Binary and Ternary Biological Complexes
Authors: Delgadillo Roberto F. , Carnes Katie A. , Valles-Villarreal Nestor , Olmos Omar , Zaleta-Rivera Kathia , Parkhurst Lawrence J.
Published: November 2020

The Stopped-Flow apparatus (SF) tracks molecular events by mixing the reactants in sub-millisecond regimes. The reaction of intrinsically or extrinsically labeled biomolecules can be monitored by recording the fluorescence, F(t), anisotropy, r(t), polarization, p(t), or FRET, F(t)FRET, traces at nanomolar concentrations. These kinetic measurements are critical to elucidate reaction mechanisms, structural information, and even thermodynamics. In a single detector SF, or L-configuration, the r(t), p(t), and F(t) traces are acquired by switching the orientation of the emission polarizer to collect the IVV and IVH signals however it requires two-shot experiments. In a two-detector SF, or T-configuration, these traces are collected in a single-shot experiment, but it increases the apparatus’ complexity and price. Herein, we present a single-detector dual-channel SF to obtain the F(t) and r(t) traces simultaneously, in which a photo-elastic modulator oscillates by 90° the excitation light plane at a 50 kHz frequency, and the emission signal is processed by a set of electronic filters that split it into the r(t) and F(t) analog signals that are digitized and stored into separated spreadsheets by a custom-tailored instrument control software. We evaluated the association kinetics of binary and ternary biological complexes acquired with our dual-channel SF and the traditional methods; such as a single polarizer at the magic angle to acquire F(t), a set of polarizers to track F(t), and r(t), and by energy transfer quenching, F(t)FRET. Our dual-channel SF economized labeled material and yielded rate constants in excellent agreement with the traditional methods.
The Evaluation of AREG, MMP-2, CHI3L1, GFAP, and OPN Serum Combined Value in Astrocytic Glioma Patients’ Diagnosis and Prognosis
Authors: Urbanavičiūtė Rūta , Skauminas Kęstutis , Skiriutė Daina
Published: November 2020

Gliomas account for approximately 70% of primary brain tumors in adults. Of all gliomas, grade IV astrocytoma, also called glioblastoma, has the poorest overall survival, with <5% of patients surviving five years after diagnosis. Due to the aggressiveness, lethal nature, and impaired surgical accessibility of the tumor, early diagnosis of the tumor and, in addition, prediction of the patient’s survival time are important. We hypothesize that combining the protein level values of highly recognizable glioblastoma serum biomarkers could help to achieve higher specificity and sensitivity in predicting glioma patient outcome as compared to single markers. The aim of this study was to select the most promising astrocytoma patient overall survival prediction variables from five secretory proteins—glial fibrillary acidic protein (GFAP), matrix metalloproteinase-2 (MMP-2), chitinase 3-like 1 (CHI3L1), osteopontin (OPN), and amphiregulin (AREG)—combining them with routinely used tumor markers to create a Patient Survival Score calculation tool. The study group consisted of 70 astrocytoma patients and 31 healthy controls. We demonstrated that integrating serum CHI3L1 and OPN protein level values and tumor isocitrate dehydrogenase 1 IDH1 mutational status into one parameter could predict low-grade astrocytoma patients’ two-year survival with 93.8% accuracy.
The Role of Façades in Solar Energy Utilization
Authors: Horn Valéria , Dudás Annamária , Terjék Anita
Published: November 2020

During the summer, the environmental impact of building operations can be reduced by optimizing ventilation and preventing heat from entering the building. Shades are used to create a comfortable temperature state in interiors without operating mechanical equipment or using low-power systems. The most significant factor in reducing energy consumption would be if the spatial delimitation of buildings utilizes solar energy in a passive or active way. At the same time, limiting the energy entering through windows reduces the temperature of interiors and the amount of energy used for cooling; thus, the phenomenon of the urban heat island would be less exacerbated. In the case of existing historical buildings, there are limited possibilities to reduce the energy consumption of the building and to protect the buildings against excessive summer heating, especially in connection with the structural appearance of the doors and windows on the façade. This paper presents the preparation of a large-scale reconstruction project based on a newly developed combined window that can significantly reduce indoor summer overheating. Designing steps of the complex retrofitting of a traditional box-type window are highlighted based on the results of closely connected literature pertaining to thermal, ventilation, shading, and acoustical phenomena. The result is a detailed structural and technological design of the reconstruction for the historical window in a combined way, as the frame, glazing, ventilation, and shading are simultaneously developed and calculated approximately; moreover, active shading is integrated. One aim of this desktop study was to demonstrate that in the case of historical buildings, it is possible and necessary to reduce heat losses during the heating season and to minimize the risk of summer overheating, and to show that the surface of the façade could be converted into an energy producer.
Bis(diphenylphosphino)methane Dioxide Complexes of Lanthanide Trichlorides: Synthesis, Structures and Spectroscopy
Authors: Bannister Robert D. , Levason William , Reid Gillian
Published: November 2020

Bis(diphenylphosphino)methane dioxide (dppmO2) forms eight-coordinate cations [M(dppmO2)4]Cl3 (M = La, Ce, Pr, Nd, Sm, Eu, Gd) on reaction in a 4:1 molar ratio with the appropriate LnCl3 in ethanol. Similar reaction in a 3:1 ratio produced seven-coordinate [M(dppmO2)3Cl]Cl2 (M = Sm, Eu, Gd, Tb, Dy, Ho, Er, Tm, Yb), whilst LuCl3 alone produced six-coordinate [Lu(dppmO2)2Cl2]Cl. The complexes have been characterised by IR, 1H and 31P{1H}-NMR spectroscopy. X-ray structures show that [M(dppmO2)4]Cl3 (M = Ce, Sm, Gd) contain square antiprismatic cations, whilst [M(dppmO2)3Cl]Cl2 (M = Yb, Dy, Lu) have distorted pentagonal bipyramidal structures with apical Cl. The [Lu(dppmO2)2Cl2]Cl has a cis octahedral cation. The structure of [Yb(dppmO2)3(H2O)]Cl3·dppmO2 is also reported. The change in coordination numbers and geometry along the series is driven by the decreasing lanthanide cation radii, but the chloride counter anions also play a role.
Association of Vitamin D Status and Physical Activity with Lipid Profile in Korean Children and Adolescents: A Population-Based Study
Authors: Song Kyungchul , Park Gihong , Choi Youngha , Oh Jun Suk , Choi Han Saem , Suh Junghwan , Kwon Ahreum , Kim Ho-Seong , Chae Hyun Wook
Published: November 2020

Dyslipidemia is one of the important influencing factors of cardiovascular health in the youth, and thus, assessment of its etiology is important. We aimed to investigate the association of dyslipidemia with vitamin D and physical activity in Korean children and adolescents. Data of 3183 subjects aged 12–18 years in the Korea National Health and Nutrition Examination Survey were analyzed. Participants were divided into subgroups according to sex, body mass index, 25-hydroxyvitamin D levels, and lipid profile. The mean 25-hydroxyvitamin D level was 16.15 ng/mL, which was below normal. In total, 79.3% of the subjects had vitamin D deficiency. Females had lower vitamin D levels and a higher incidence of dyslipidemia compared to males. Vitamin D deficiency was significantly associated with high density lipoprotein cholesterol (HDL-C) levels. The low HDL-C group consisted of a higher proportion of subjects with vitamin D deficiency and low physical activity. This study suggests that vitamin D deficiency is prevalent in Korean children and adolescents. Vitamin D deficiency and low physical activity are related with low HDL-C levels. Maintaining sufficient vitamin D levels and physical activity may help prevent dyslipidemia.
A Taxonomy of Blockchain Consensus Methods
Authors: Nijsse Jeff , Litchfield Alan
Published: November 2020

For a blockchain, consensus is the foundation protocol that enables cryptocurrencies such as Bitcoin to maintain state. Additionally, to ensure safety and liveness for a publicly accessible and verifiable ledger, fault tolerance must be robust. However, there appears to be a degree of misunderstanding about how consensus is applied across blockchains. To assist researchers considering variations between them, this study presents a rational classification of consensus methods applied to current blockchains. The study provides a survey of 19 methods classified by the scarce resource they employ: clock-cycles, bits, tokens, votes, time, and biometrics. Blockchain implementations are split between consensus algorithms requiring proof of resource and those that use majority voting to update the ledger.
Layered Double Hydroxides
Authors: Prestopino Giuseppe , Arrabito Giuseppe
Published: November 2020

The impact of layered double hydroxides (LDHs) within the multidisciplinary fields of materials sciences, physics, chemistry, and biology is rapidly growing, given their easiness of synthesis, flexibility in composition, tunable biocompatibility and morphology [...]
A Coloring Study of the Ga Richest Alkali Gallides: New In- and Hg-Containing Gallides with the RbGa7- and the K3Ga13-Type Structure
Authors: Falk Martha , Wendorff Marco , Röhr Caroline
Published: November 2020

The Ga-rich gallides of the alkali metals present an interesting, yet still scarcely investigated case of polyanionic cluster compounds with subtle variations in the character of their chemical bonding. In the present work, the Ga richest phases K3Ga13, RbGa7, and CsGa7, which are formally electron-precise Zintl/Wade cluster compounds, are systematically studied with respect to a partial substitution of Ga by In and Hg. The pure hepta-gallides AGa7 (A = Rb/Cs; R3¯m), which were formerly obtained from Ga-rich melts in powder form only, were crystallized from Hg-rich melts. Herein, up to 9.9/13.6% (Rb/Cs) of Ga could be substituted by In, which partly takes the four-bonded [M2] dumbbells connecting layers of Ga-icosahedra. Even though the structures are electron precise, the pseudo band gap does not coincide with the Fermi level. In the most Ga-rich potassium compound K3Ga13 (Cmcm) only 1.2% of In and 2.7% of Hg could be incorporated. Although Rb3Ga13 remains unknown, ternary variants containing 5.2 to 8.2% In could be obtained; this structure is also stabilized by a small Hg-proportion. The likewise closed-shell 3D polyanion consists of all-exo-bonded Ga-icosahedra and closo [Ga11] clusters, which are connected by two tetrahedrally four-bonded Ga− and a trigonal-planar three-bonded Ga0. The aspects of the electronic structures and the site-specific Ga↦Hg/In substitution in the polyanion (“coloring”) are discussed for the title compounds and other mixed Ga/In trielides.
Chemerin Is a Valuable Biomarker in Patients with HCV Infection and Correlates with Liver Injury
Authors: Peschel Georg , Grimm Jonathan , Gülow Karsten , Müller Martina , Buechler Christa , Weigand Kilian
Published: November 2020

Hepatitis C virus (HCV)-induced inflammation contributes to progressive liver disease. The chemoattractant protein chemerin is associated with systemic inflammation. We hypothesized that chemerin is a biomarker that predicts the severity of liver disease in HCV patients. Furthermore, we investigated whether serum chemerin levels change during the course of HCV treatment using direct-acting antivirals (DAAs). Therefore, we measured serum concentration of chemerin in a cohort of 82 HCV-infected patients undergoing DAA treatment. Serum chemerin was positively associated with leukocyte count and negatively with markers of hepatic function and the model of end-stage liver disease (MELD) score. Low circulating chemerin levels significantly correlated with advanced liver fibrosis and cirrhosis as measured by the fibrosis-4 (FIB-4) score, the aminotransferase/platelet (AST/PLT) ratio index (APRI) score and the non-alcoholic fatty liver disease (NAFLD) score. Chemerin did not correlate with viral load or viral genotype. Treatment with DAAs did not improve MELD score and leukocyte count within the observation period, up to three months after the end of DAA treatment. Accordingly, chemerin levels remained unchanged during the treatment period. We conclude that low circulating chemerin is a noninvasive biomarker for hepatic dysfunction and advanced liver fibrosis and cirrhosis in HCV infection.
Heat Transfer Analysis of Timber Windows with Different Wood Species and Anatomical Direction
Authors: Ahn Namhyuck , Park Sanghoon
Published: November 2020

When assessing the hygrothermal performance of timber windows, it is important to apply the unique thermal conductivity of wood by each wood species as well as an anatomical direction within the same material as they affect the performance and long-term durability of products. A series of heat transfer analyses of window frames using THERM and WINDOW along with measurements on the thermal conductivity of five hardwoods using laser flash apparatus (LFA) was performed to compare and evaluate heat transmittance (U-value) and condensation resistance (CR) of three types of timber and hybrid timber windows. For each window type, 6.1 to 10.3% of the maximum difference in the heat transmittance among cases was calculated. Besides, a linear correlation was found between the U-value and the CR for most cases; thus, the selection of wood species and anatomical direction would improve the hygrothermal performance of timber windows overall. The results also indicated that there were some cases where the overall CR of windows did not improve because the U-value of the glazing system was not sufficiently low.
Mitigation of Deer Herbivory in Temperate Hardwood Forest Regeneration: A Meta-Analysis of Research Literature
Authors: Redick Caleb H. , Jacobs Douglass F.
Published: November 2020

Ungulate herbivory poses global challenges to forest regeneration. Deer, in combination with other biotic and abiotic factors, threaten to shift forest composition away from palatable hardwoods, such as oaks (Quercus spp.), and cause regeneration failure in some cases. Many studies have assessed methods to reduce or manage browse, but comprehensive analyses of the relative effectiveness of these techniques among published experiments are lacking. We synthesized the literature describing the results of methods to reduce deer browsing impacts, and assessed the effectiveness of deer browse management methods in controlling damage to hardwood forest regeneration. Specifically, we systematically analyzed results from 99 studies that used repellents, physical barriers, lethal population control, timber harvests, facilitation by neighboring plants, or fertilizer to affect browse, survival, or height growth of hardwood seedlings. Across studies, browse was reduced (mean effect size and confidence intervals) with the following: Fencing −3.17 (CI: −4.00–−1.31), shelters −1.28 (CI: −2.02–−0.67), cages −1.48 (CI: −3.14–−0.62), facilitation from neighboring plants −0.58 (CI: −1.11–−0.13), repellents −0.45 (CI: −0.56–−0.21), hunting −0.99 (CI: −1.51–−0.26). These methods each had positive effects on seedling height growth (except for repellents), and cages, timber harvests, fences, and mesh sleeves had positive effects on survival. Logging slash had no effect on browse incidence (−0.05, CI: −0.97–0.19). Fertilizer applied during seedling establishment increased browse (0.13, CI: 0.11–0.21), and did not affect height growth. We conclude that fences or other physical barriers best control for the effects of deer, but facilitation by surrounding vegetation, logging slash, hunting, habitat management through timber harvest, and certain repellents may also be moderately effective. Discrepancies between browse effectiveness and relative costs suggest that economic analyses should be developed to help to guide prescriptions for management.
Towards the Understanding of Humpback Whale Tubercles: Linear Stability Analysis of a Wavy Flat Plate
Authors: Owen Miles , Frendi Abdelkader
Published: November 2020

The results from a temporal linear stability analysis of a subsonic boundary layer over a flat plate with a straight and wavy leading edge are presented in this paper for a swept and un-swept plate. For the wavy leading-edge case, an extensive study on the effects of the amplitude and wavelength of the waviness was performed. Our results show that the wavy leading edge increases the critical Reynolds number for both swept and un-swept plates. For the un-swept plate, increasing the leading-edge amplitude increased the critical Reynolds number, while changing the leading-edge wavelength had no effect on the mean flow and hence the flow stability. For the swept plate, a local analysis at the leading-edge peak showed that increasing the leading-edge amplitude increased the critical Reynolds number asymptotically, while the leading-edge wavelength required optimization. A global analysis was subsequently performed across the span of the swept plate, where smaller leading-edge wavelengths produced relatively constant critical Reynolds number profiles that were larger than those of the straight leading edge, while larger leading-edge wavelengths produced oscillating critical Reynolds number profiles. It was also found that the most amplified wavenumber was not affected by the wavy leading-edge geometry and hence independent of the waviness.
Modelling Microlayer Formation in Boiling Sodium
Authors: Giustini Giovanni , Kim Hyungdae , I. Issa Raad , J. Bluck Michael
Published: November 2020

During boiling at a solid surface, it is often the case that a liquid layer of a few microns of thickness (’microlayer’) is formed beneath a bubble growing on the heated surface. Microlayers have been observed forming beneath bubbles in various transparent fluids, such as water and refrigerants, subsequently depleting due to evaporation, thus contributing significantly to bubble growth and possibly generating the majority of vapor in a bubble. On the other hand, boiling of opaque fluids, such as liquid metals, is not amenable to optical observations, and microlayers have not yet been observed in liquid metals. Among that class of fluids is sodium, suitable as a coolant for nuclear reactors and as the working fluid in phase-change solar power receivers. In order to support these applications, it is necessary to understand the boiling behavior of sodium and identify the parameters that might influence microlayer formation during boiling of this important fluid. This paper presents simulations of the hydrodynamics of sodium vapor bubble growth at a surface. An interface capturing flow solver has been implemented in the OpenFOAM code and used to predict the behavior of a sodium vapor bubble near a solid surface in typical boiling conditions. The methodology has been validated using recently reported direct experimental observations of microlayer formation in water and then applied to sodium boiling cases. Simulations indicate that microlayers are formed in sodium in a similar fashion to water. Comparison of simulation results with an extant algebraic model of microlayer formation showed good agreement, which increases confidence in the current predictions of microlayer formation. Typical values of microlayer thickness thus computed indicate that the microlayer is likely to play an important role during bubble growth in sodium.
“Burrata di Andria” PGI Cheese: Physicochemical and Microbiological Features
Authors: Di Cerbo Alessandro , Miraglia Dino , Marino Leonardo , Stocchi Roberta , Loschi Anna , Fisichella Stefano , Cammertoni Natalina , Menchetti Laura , Farneti Silvana , Ranucci David , Branciari Raffaella , Rea Stefano
Published: November 2020

In the last century, the exponential increase of industrial food production led to the disappearance of “Italian traditional niche products”. However, national regulations allowed the preservation of several of these products, including the burrata cheese. Twenty-one samples from three different batches of “Burrata di Andria” Protected Geographical Indication (PGI) were purchased from dairy factories of the PGI consortium. Moisture value of PGI Burrata cheese was significantly higher than that before the PGI release. Moreover, a significantly lower NaCl value was detected in PGI raw milk Burrata cheeses with respect to non-PGI ones, while an opposite situation was detected in pasteurized milk Burrata cheeses. As for pH, in all PGI products lower values were observed with respect to non-PGI products, which resulted significant only in pasteurized ones. No Salmonella spp., Listeria monocytogenes, and Bacillus cereus were detected, while nine samples were positive for a nonpathogenic strain of Yersinia enterocolitica. Total viable count (TVC) and Escherichia coli resulted significantly lower in pasteurized than in raw milk PGI Burrata cheese samples. Although samples analyzed can be considered microbiologically safe, these were borderline and/or unsatisfactory for E. Coli and coagulase-positive staphylococci (CPS) according to process hygiene criteria established by European regulation. Therefore, different strategies should be adopted to improve products hygiene in the considered dairy factories.
A New Methodology for Measuring Tsunami Resilience Using Theory of Springs
Authors: Pushpalal Dinil , Suzuki Atsushi
Published: November 2020

Resilience is a deeply rooted word in theory of elasticity, which is firstly introduced to English by Thomas Young in 1807 in his treatise “A course of lectures on natural philosophy and the mechanical arts”. However, recently it is frequently used in ecology, economics, social sciences, and as everyone knows in the disaster literature. The purpose of this article is to investigate the mechanical background of word resilience, discuss lessons we could learn from the theory of elasticity for evaluating tsunami resilience, and finally, to propose a new mathematical model based on theory of springs. The mathematical model is in compliance with a pragmatic conceptual framework for evaluating resilience. The effective resilience of a given area can be calculated by aggregation of three components namely, onsite capacity, instantaneous survivability, and recovery potential of the area. The authors suggest that the magnitude of each component depends on socioeconomic, infrastructural and geographical factors of the area considered. Here, we show that aggregation of the individual components can be done in compliance with the theory of springs by analogizing effective tsunami resilience to effective spring constant. The mathematical model will be useful for evaluating the resilience of townships to hydrological disasters and also planning resilient townships, specifically to tsunami.
Effect of High CO2 Treatment and MA Packaging on Sensory Quality and Physiological-Biochemical Characteristics of Green Asparagus (Asparagus officinalis L.) during Postharvest Storage
Authors: Wang Li-Xia , Choi In-Lee , Kang Ho-Min
Published: November 2020

Green asparagus is vulnerable to thrips that carry microorganisms and cause deterioration in quality. The effects of 60% CO2 treatment, which is used to kill thrips, combined with perforated (P) or modified atmosphere (MA) packages during cold storage, on the sensory quality and physiological–biochemical characteristics of asparagus were investigated. MA packaging yielded an asparagus shelf-life five days longer than P packaging. The 60% CO2 treatment for 48 h at 4 °C packaged with MA film (CO2-48 h-4 °C-MA) showed a lower number of aerobic bacteria, yeast, and mold. Yellowing of asparagus was retarded, as shown by higher hue angle and chlorophyll content and lower chlorophyllase activity. Also, CO2-48 h-4 °C-MA treatment inhibited the reduction of soluble solids content in asparagus. Likewise, all high CO2 treatments showed lower electrolyte leakage (EL), with CO2-48 h-4 °C-MA demonstrating the minimum EL. The effectiveness of high CO2 on maintaining sensory qualities was observed, with a score higher than 3.0. In conclusion, CO2-48 h-4 °C-MA treatment during cold storage was effective for maintaining post-harvest sensory qualities and physiological–biological traits of asparagus, and provided strong inhibition of microflora growth during the storage period.
Document Summarization Based on Coverage with Noise Injection and Word Association
Authors: Kim Heechan , Lee Soowon
Published: November 2020

Automatic document summarization is a field of natural language processing that is rapidly improving with the development of end-to-end deep learning models. In this paper, we propose a novel summarization model that consists of three methods. The first is a coverage method based on noise injection that makes the attention mechanism select only important words by defining previous context information as noise. This alleviates the problem that the summarization model generates the same word sequence repeatedly. The second is a word association method to update the information of each word by comparing the information of the current step with the information of all previous decoding steps. According to following words, this catches a change in the meaning of the word that has been already decoded. The third is a method using a suppression loss function that explicitly minimizes the probabilities of non-answer words. The proposed summarization model showed good performance on some recall-oriented understudy for gisting evaluation (ROUGE) metrics compared to the state-of-the-art models in the CNN/Daily Mail summarization task, and the results were achieved with very few learning steps compared to the state-of-the-art models.
Erratum: Hadjixenophontos, E.; et al. A Review of the MSCA ITN ECOSTORE—Novel Complex Metal Hydrides for Efficient and Compact Storage of Renewable Energy as Hydrogen and Electricity. Inorganics 2020, 8, 17
Authors: Hadjixenophontos Efi , Dematteis Erika Michela , Berti Nicola , Wołczyk Anna Roza , Huen Priscilla , Brighi Matteo , Le Thi Thu , Santoru Antonio , Payandeh SeyedHosein , Peru Filippo , Dao Anh Ha , Liu Yinzhe , Heere Michael
Published: November 2020

The authors wish to make the following corrections to this paper [...]
Genetic and Ecological Relationships of Anastrepha ludens (Diptera: Tephritidae) Populations in Southern Mexico
Authors: Ruiz-Montoya Lorena , Vallejo Rodrigo Verónica , Haymer David , Liedo Pablo
Published: November 2020

Knowledge of the influence of evolutionary factors that promote either the differentiation or cohesion of pest insect populations is critical for the improvement of control strategies. Here, we explore the extent to which genetic differentiation occurs between populations of the Mexican fruit fly, Anastrepha ludens, in association with four plant hosts (Citrus sinensis, C. paradisi, Mangifera indica and Casimiroa edulis) in the Soconusco region of Chiapas (Mexico). Using variants from six enzymatic loci, we obtained measures of genetic diversity for three sample arrangements: (1) by sex per locality, (2) by locality and (3) by host. The extent of genetic differentiation in populations was assessed using the Analyses of Molecular Variance (AMOVA) method for each array of samples, and moderate to high levels of genetic variation were observed between the sexes, as well as among localities and host plants. A Bayesian approach was then used to assess any population structure underlying the genetic data we obtained, but this analysis showed no significant structuring due to locality or host plant. We also considered whether the observed genotypic frequencies in male and females matched those expected under a hypothesis of random mating. Here we found significant deviations from expected genotypic frequencies, suggesting that sexual selection is acting on these populations. Overall, our results indicate that sexual selection, along with the presence of some heterogeneity in environments provided by both geographical factors and availability of host plants, has influenced the evolution of pest populations in this region of Mexico. Implications for area-wide pest management strategies are discussed.
Longitudinal Imaging Using PET/CT with Collagen-I PET-Tracer and MRI for Assessment of Fibrotic and Inflammatory Lesions in a Rat Lung Injury Model
Authors: Mahmutovic Persson Irma , Fransén Pettersson Nina , Liu Jian , Falk Håkansson Hanna , Örbom Anders , In ’t Zandt René , Gidlöf Ritha , Sydoff Marie , von Wachenfeldt Karin , Olsson Lars E.
Published: November 2020

Non-invasive imaging biomarkers (IBs) are warranted to enable improved diagnostics and follow-up monitoring of interstitial lung disease (ILD) including drug-induced ILD (DIILD). Of special interest are IB, which can characterize and differentiate acute inflammation from fibrosis. The aim of the present study was to evaluate a PET-tracer specific for Collagen-I, combined with multi-echo MRI, in a rat model of DIILD. Rats were challenged intratracheally with bleomycin, and subsequently followed by MRI and PET/CT for four weeks. PET imaging demonstrated a significantly increased uptake of the collagen tracer in the lungs of challenged rats compared to controls. This was confirmed by MRI characterization of the lesions as edema or fibrotic tissue. The uptake of tracer did not show complete spatial overlap with the lesions identified by MRI. Instead, the tracer signal appeared at the borderline between lesion and healthy tissue. Histological tissue staining, fibrosis scoring, lysyl oxidase activity measurements, and gene expression markers all confirmed establishing fibrosis over time. In conclusion, the novel PET tracer for Collagen-I combined with multi-echo MRI, were successfully able to monitor fibrotic changes in bleomycin-induced lung injury. The translational approach of using non-invasive imaging techniques show potential also from a clinical perspective.
Incidence and Risk Factors of Alcohol Relapse after Liver Transplantation: Analysis of Pre-Transplant Abstinence and Psychosocial Features
Authors: Yu Tien-Wei , Chen Yu-Ming , Wang Chih-Chi , Lin Chih-Che , Huang Kuang-Tzu , Liu Yueh-Wei , Hsu Li-Wen , Li Wei-Feng , Chan Yi-Chai , Chen Chao-Long , Chen Chien-Chih
Published: November 2020

Alcohol-associated liver disease (ALD) is a common indication for liver transplantation (LT). Alcohol relapse after LT is associated with graft loss and worse prognosis. Over the past 20 years, the number and prevalence of living donor liver transplantations (LDLTs) have increased in Taiwan. The aims of this retrospective study are to analyze the incidence and risk factors of alcohol relapse after LT at a single center in Taiwan. A total of 98 patients with ALD who underwent LT from January 2012 to December 2018 were retrospectively evaluated by chart review. Pre-transplant characteristics as well as psychosocial and alcoholic history were used to test the possible associations among the risk factors studied and post-LT alcohol relapse. The incidence of post-LT alcohol relapse was 16.3%. The median duration of alcohol relapse after liver transplantation was 28.1 months (range: 1–89.4 months). The cumulative incidence was 12% and 19% at 1 year and 3 years after LT, respectively. The most powerful risk factors were a pre-LT abstinence period less than 6 months and younger age of starting alcohol. For predicting alcohol relapse, the accuracy rate of abstinence less than 6 months was up to 83.7%. In summary, pre-abstinence period plays a role in predicting post-LT alcohol relapse. Post-LT interventions should be considered specifically for the patients with short abstinence period. Long-term follow-up, patient-centered counseling, and enhancement of healthy lifestyle are suggested to prevent alcohol relapse.
Risk of Developing Pancreatic Cancer in Patients with Chronic Pancreatitis
Authors: Vujasinovic Miroslav , Dugic Ana , Maisonneuve Patrick , Aljic Amer , Berggren Robin , Panic Nikola , Valente Roberto , Pozzi Mucelli Raffaella , Waldthaler Alexander , Ghorbani Poya , Kordes Maximilian , Hagström Hannes , Löhr Johannes-Matthias
Published: November 2020

Background: Patients with chronic pancreatitis (CP) have an increased risk of developing pancreatic ductal adenocarcinoma (PDAC). We present data on PDAC in one of the most extensive European single-centre cohort studies of patients with CP. Methods: Retrospective analysis of prospectively collected data of patients with CP was performed. Aetiology of CP was determined according to the M-ANNHEIM classification system and only patients with definite CP > 18 years at data analysis were included. The final dataset included 581 patients with definite CP diagnosed between 2003 and 2018. Results: At CP diagnosis, there were 371 (63.9%) males and 210 (36.1%) females (median age 57 years, range 2–86). During 3423 person-years of observation, six pancreatic cancers were diagnosed (0.2% year). The mean time between diagnosis of CP and the occurrence of PDAC was 5.0 years (range 2.7–8.6). None of the cancer patients had a family history of PDAC. Diabetes mellitus (DM) was present in five of six (83.3%) patients with PDAC: in three patients before and in two after CP diagnosis. Clinical/laboratory signs of pancreatic exocrine insufficiency (PEI) were present in five of six (83.3%) patients with PDAC: in two at diagnosis of CP and in three after diagnosis. The mean survival time was 4 months after the diagnosis of PDAC (range 0.5–13). PDAC occurred significantly more often (p < 0.001) in two groups of patients without previous acute pancreatitis (AP): 2 of 20 patients (10%) with low body mass index (BMI) and PEI and in 3 of 10 (30%) patients with high BMI and DM at diagnosis of CP. Conclusions: Patients with CP have a high risk of developing PDAC, although risk is low in absolute terms. Our data suggest the possibility of defining subgroups of patients with a particularly elevated risk of PDAC. Such a possibility would open a path to personalised decision making on initiation of PDAC surveillance of patients with no previous episode of AP, (i) with low BMI and PEI, or (ii) elevated BMI and DM.
Ligninolytic Enzyme Production and Decolorization Capacity of Synthetic Dyes by Saprotrophic White Rot, Brown Rot, and Litter Decomposing Basidiomycetes
Authors: Eichlerová Ivana , Baldrian Petr
Published: November 2020

An extensive screening of saprotrophic Basidiomycetes causing white rot (WR), brown rot (BR), or litter decomposition (LD) for the production of laccase and Mn-peroxidase (MnP) and decolorization of the synthetic dyes Orange G and Remazol Brilliant Blue R (RBBR) was performed. The study considered in total 150 strains belonging to 77 species. The aim of this work was to compare the decolorization and ligninolytic capacity among different ecophysiological and taxonomic groups of Basidiomycetes. WR strains decolorized both dyes most efficiently; high decolorization capacity was also found in some LD fungi. The enzyme production was recorded in all three ecophysiology groups, but to a different extent. All WR and LD fungi produced laccase, and the majority of them also produced MnP. The strains belonging to BR lacked decolorization capabilities. None of them produced MnP and the production of laccase was either very low or absent. The most efficient decolorization of both dyes and the highest laccase production was found among the members of the orders Polyporales and Agaricales. The strains with high MnP activity occurred across almost all fungal orders (Polyporales, Agaricales, Hymenochaetales, and Russulales). Synthetic dye decolorization by fungal strains was clearly related to their production of ligninolytic enzymes and both properties were determined by the interaction of their ecophysiology and taxonomy, with a more relevant role of ecophysiology. Our screening revealed 12 strains with high decolorization capacity (9 WR and 3 LD), which could be promising for further biotechnological utilization.
Saving Lives and Changing Minds with Twitter in Disasters and Pandemics: A Literature Review
Authors: Seddighi Hamed , Salmani Ibrahim , Seddighi Saeideh
Published: November 2020

Twitter is a major tool for communication during emergencies and disasters. This study aimed to investigate Twitter use during natural hazards and pandemics. The included studies reported the role of Twitter in disasters triggered by natural hazards. Electronic databases were used for a comprehensive literature search to identify the records that match the mentioned inclusion criteria published through May 2020. Forty-five articles met the selection criteria and were included in the review. These indicated ten functions of Twitter in disasters, including early warning, dissemination of information, advocacy, assessment, risk communication, public sentiment, geographical analysis, charity, collaboration with influencers and building trust. Preventing the spread of misinformation is one of the most important issues in times of disaster, especially pandemics. Sharing accurate, transparent and prompt information from emergency organizations and governments can help. Moreover, analyzing Twitter data can be a good way to understand the mental state of the community, estimate the number of injured people, estimate the points affected by disasters and model the prevalence of epidemics. Therefore, various groups such as politicians, government, nongovernmental organizations, aid workers and the health system can use this information to plan and implement interventions.
Investigation of Finishing Aluminum Alloy A5052 Using the Magnetic Abrasive Finishing Combined with Electrolytic Process
Authors: Xing Baijun , Zou Yanhua
Published: November 2020

The magnetic abrasive finishing combined with electrolytic (EMAF) process was proposed to improve the finishing efficiency of the traditional magnetic abrasive finishing (MAF) process. Since the EMAF process contains electrolysis reactions, the machining mechanism of processing different metal is different. In this paper, a series of experiments were conducted to explore the feasibility of using the compound processing tool to finish aluminum alloy A5052, and to preliminary explore the machining mechanism. Surface roughness and material removal are used to evaluate the finishing effect and the finishing efficiency, respectively. The EMAF processing current curve is used to evaluate and analyze the EMAF process. The feasibility of the EMAF processing is proved by the analysis of simulations and the experimental results. Finally, through a series of exploration experiments and parameter optimization experiments, the main conclusions are as follows: (1) Compared with the traditional MAF process, when finishing the surface of aluminum alloy A5052 by the same compound processing tool and at the same experimental conditions (except the electrolysis conditions), the EMAF process, which includes electrolysis reactions, can achieve higher finishing efficiency. (2) In this study, when the working gap is 1 mm and the concentration of NaNO3 solution is 15%, the recommended processing voltage is about 3.4 V.
On the Affine Image of a Rational Surface of Revolution
Authors: Alcázar Juan G.
Published: November 2020

We study the properties of the image of a rational surface of revolution under a nonsingular affine mapping. We prove that this image has a notable property, namely that all the affine normal lines, a concept that appears in the context of affine differential geometry, created by Blaschke in the first decades of the 20th century, intersect a fixed line. Given a rational surface with this property, which can be algorithmically checked, we provide an algorithmic method to find a surface of revolution, if it exists, whose image under an affine mapping is the given surface; the algorithm also finds the affine transformation mapping one surface onto the other. Finally, we also prove that the only rational affine surfaces of rotation, a generalization of surfaces of revolution that arises in the context of affine differential geometry, and which includes surfaces of revolution as a subtype, affinely transforming into a surface of revolution are the surfaces of revolution, and that in that case the affine mapping must be a similarity.
Topology Optimization of Elastoplastic Behavior Conditions by Selectively Suppressing Plastic Work
Authors: Lee Eun-Ho , Kim Tae-Hyun
Published: November 2020

This work conducted topology optimization with an implicit analysis of elastoplastic constitutive equation in order to design supporting structures for unexpected heavy loading conditions. In this topology optimization model, plastic work was extracted from strain energy and selectively employed in the objective function according to deformation mode. While strain energy was minimized in elastic deformation areas, in elastoplastic deformation areas, the plastic work was minimized for the purpose of suppressing plastic deformation. This method can focus on suppressing plastic strain in the plastic deformation zone with maintaining elastic stiffness in the elastic deformation zone. These formulations were implemented into MATLAB and applied to three optimization problems. The elastoplastic optimization results were compared to pure elastic design results. The comparison showed that structures designed with accounting for plastic deformation had a reinforced area where plastic deformation occurs. Finally, a finite element analysis was conducted to compare the mechanical performances of structures with respect to the design method.
Local Limit Theorem for the Multiple Power Series Distributions
Authors: L. Yakymiv Arsen
Published: November 2020

We study the behavior of multiple power series distributions at the boundary points of their existence. In previous papers, the necessary and sufficient conditions for the integral limit theorem were obtained. Here, the necessary and sufficient conditions for the corresponding local limit theorem are established. This article is dedicated to the memory of my teacher, professor V.M. Zolotarev.
Relationship between Maximum Tongue Pressure Value and Age, Occlusal Status, or Body Mass Index among the Community-Dwelling Elderly
Authors: Suzuki Hiroki , Ayukawa Yasunori , Ueno Yoko , Atsuta Ikiru , Jinnouchi Akio , Koyano Kiyoshi
Published: November 2020

Background and objectives: In an aging society, the maintenance of the oral function of the elderly is of importance for the delay or prevention of frailty and long-term care. In the present study, we focused on the maximum tongue pressure (MTP) value and analyzed the relationship between MTP and age, occlusal status, or body mass index (BMI). Materials and Methods: This one-center observatory study was conducted using a cohort consisting of 205 community-dwelling outpatients over 65 years old. The MTP values of all subjects were measured using a commercially available tongue pressure measurement device and statistically analyzed. In addition, the correlation between MTP value and BMI was analyzed. Results: The MTP value decreased with age, especially in subjects classified as Eichner B and C. The difference in occlusal status did not show any statistically significant influence on MTP value. The correlation between BMI and MTP value was indicated in the tested groups other than an age of 65–74 and Eichner A groups. Conclusions: Although MTP value decreased with age, the difference in occlusal status did not have an impact on MTP value. The correlation between BMI and MTP value was not shown in the youngest group or a group with sufficient occlusal units. The results presented in the present study may imply that, even if MTP is low, younger age and/or better occlusal status compensate for the inferior MTP value in the cohort studied.
Differentiation of Tumorigenic C6 Glioma Cells Induced by Enhanced IL-6 Signaling
Authors: Chu Inn-Ray , Pan Rong-Long , Yang Chung-Shi
Published: November 2020

Background and objectives: Cancer stem cells (CSCs) are obstacles to cancer therapy due to their therapeutic resistance, ability to initiate neoplasia, and roles in tumor relapse and metastasis. Efforts have been made to cure CSCs, such as the use of differentiation therapy, which induces cancer stem-like cells to undergo differentiation and decrease their tumorigenicity. Interleukin 6 (IL-6) upregulates the expression of glial fibrillary acidic protein (GFAP) in C6 glioma cells, indicating that it is able to induce the differentiation of these cells. The C6 glioma cell line forms a high percentage of cancer stem-like cells, leading us to speculate whether IL-6 signaling could modulate the differentiation of tumorigenic C6 glioma cells. However, we observed that IL-6 alone could not efficiently induce the differentiation of these cells. Therefore, different IL-6 signaling elicitors, including IL-6 alone, a combination of IL-6 and soluble IL-6 receptor (IL-6/sIL-6R), and tumor necrosis factor-α (TNF-α) plus IL-6/sIL-6R (TNF-α/IL-6/sIL-6R), were evaluated for their potential use in differentiation therapy. Materials and Methods: The potential of IL-6 signaling elicitors in differentiation therapy were examined by assessing changes in biomarker levels, the rate of cell proliferation, and tumorigenicity, respectively. Results: Enhanced IL-6 signaling could effectively induce C6 glioma cell differentiation, as determined by observed variations in the expression of differentiation, cell cycle, and stem cell biomarkers. Additionally, the total cell population and the tumorigenicity of glioma cells were all considerably reduced after TNF-α/IL-6/sIL-6R treatment. Conclusions: Our findings provide evidence that enhanced IL-6 signaling can efficiently promote tumorigenic C6 glioma cells to undergo differentiation.
Thermomechanical Treatment of Martensitic Stainless Steels Sheets and Its Effects on Their Deep Drawability and Resulting Hardness in Press Hardening
Authors: Meza-García Enrique , Birnbaum Peter , Landgraf Pierre , Grund Thomas , Lampke Thomas , Kräusel Verena
Published: November 2020

The deep drawability of three Martensitic Stainless Steels (MSS) alloys under conventional press hardening thermomechanical process conditions was investigated. The three alloys differ in the content of the main elements C and Cr. Firstly, the metallurgical properties of the alloys were determined, i.e., the phase mass fraction diagrams and the concentration of alloying elements in solid solution at the austenitic temperatures with help of the JMatPro® software version 7.0. Derived from this analysis, specific thermomechanical process parameters were defined to evaluate experimentally and numerically the hot sheet formability of the alloys during the deep drawing process. The hot deep drawability of the MSS alloys was experimentally assessed. The hot deep drawability was evaluated with the resulting maximum drawing depth values. In general, all three alloys developed very good formability at forming temperatures between 700 and 900 °C. However, they are susceptible to chemical composition, austenization temperature, dwell time, and flange gap. The hot formability behavior of the alloys as well as the resulting hardness showed very good concordance with the calculated metallurgical values. Finally, a numerical analysis was conducted using Simufact Forming® 15.0 software. The interval time during hot blank transfer to the tool determines the initial and final forming temperature. The effect of the time interval on the forming temperature was analyzed numerically and validated experimentally. It was also possible to determine the maximum level of plastic strain in the deep drawn cup.