bluuebunny/crossref_metadata_2025 huggingface dataset:
Case report of optimizer implantation to modulate cardiac contractility in patients with chronic heart failure and atrial fibrillation
Authors: Pushkareva AE , Blagodarov SI , Abdrakhmanov RE , Plotnikova MR , Dolganov AA , Mustafina IA , Zagidullin NSh
Published: March 2023

A cardiac contractility modulation device is a new treatment used in patients with heart failure. CCM therapy is associated with an increase in physical activity tolerance, improved quality of life, reduced hospitalizations due to heart failure, and reverse remodeling of the left ventricle in patients with systolic heart failure. In this case, the clinical benefit of cardiac contractility modulation in a patient with chronic heart failure, atrial fibrillation and postinfarction left ventricle aneurysm was reported. Development of postinfarction left ventricle aneurysm in patients with reduced ejection fraction is associated with a high risk of surgical complications. However, an adequate assessment of the functional reserve of the left ventricle myocardium and the choice of surgical correction method allows for receiving favorable outcomes of surgery. We present a case of a successful combination of interventional and surgical treatment of a patient with heart failure and post-infarction left ventricular aneurysm.
Robotic assisted laparoscopic radical prostatectomy with the new surgical robot HugoTM RAS in a man with proctocolectomy and restorative ileal pouch-anal anastomosis. The first European urological step-by-step procedure
Authors: Totaro A , Scarciglia E , Ragonese M , Cretì A , Bizzarri FP , Campetella M , Gandi C , Palermo G , Racioppi M , Sacco E
Published: March 2023

Robotic-Assisted Laparoscopic Radical Prostatectomy (RARP) is the gold standard surgical treatment for localized clinically significant prostate cancer. In patients undergone previous abdominal or pelvic surgery, it can be difficult to perform and it is also contraindicated execution of radiotherapy. We have described step-by-step the first case of RARP performed with the robotic system HugoTM RAS in a patient previously subjected to proctocolectomy and ileal pouch-anal anastomosis packaging for ulcerative colitis. The procedure was found to be safe and feasible, with intra- and post-operative data overlapping those of the same procedure in the general population. Execution by an experienced surgeon is in any case recommended.
EdU Immunohistochemistry using Click-it reaction v1
Authors: Gola Anita
Published: March 2023

This protocol allows users to image histology tissue using basic immunohistochemistry techniques in combination with EdU capture (using the Click-it reaction system). EdU (5-ethynyl-2′-deoxyuridine) will capture S-phase cells with the thymidine analogue, allowing to take a snap-shot of actively diving cells. In this assay the modified thymidine analogue EdU is efficiently incorporated into newly synthesized DNA and fluorescently labeled with a bright, photostable Alexa Fluor™ dye in a fast, highly-specific click reaction. This fluorescent labeling of proliferating cells is accurate and compatible with antibody methods due to the mild click protocol.
FPCount protocol - in-lysate (purification free) protocol v2
Authors: Csibra Eszter
Published: March 2023

FPCount is a complete protocol for fluorescent protein calibration, consisting of: FP expression and production of cell lysates. FP concentration determination in a microplate reader. FP fluorescence quantification in a microplate reader. Results can be analysed with the corresponding R package, FPCountR. This in-lysate version of the protocol uses the ECmax protein quantification protocol of FPs in lysates and does not require His-tag purification of the FPs. Note that it is only suitable for FPs with entries in FPbase. This is Version 2 of this protocol on protocols.io. Version 1 was published in Dec 2021. Version 2 (Mar 2023) brings the protocols.io protocol in line with the final protocol included in our FPCountR publication (Nov 2022). As of March 2023, this is the latest version of our FPCount wet lab protocol, and is the ONLY protocol recommended for routine calibration. All previous versions of the 'in-lysate protocol' and all other variations (ie. the 'full protocol' and 'short protocol') are not recommended for routine calibration.
Influence of Parenting Stress on Depression among Single Parents with Preschool Children during the COVID-19 Pandemic: Focusing on the Moderating Effect of Self-rated Health Status
Authors: Lee RaeHyuck
Published: March 2023

Background: This study aimed to verify the influence of parental stress on depression among single parents with preschool children during the COVID-19 pandemic and the moderating effect of self-rated health status.Methods: The study conducted regression analyses with a sample of 335 single parents raising children under seven years old from the raw data of the Study on the Status of Sole-parent Families conducted by the Korean Ministry of Gender Equality and Family in 2021.Results: Single parents’ parenting stress had a positive and significant influence on their depression, moderated by their self-rated health status. That is, the influence of parenting stress on depression was more pronounced for parents who rated their health status as not good compared with those who rated their health status as good.Conclusions: Based on the findings, the discussion suggested interventions for dealing with depression among single parents with preschool children.
The Double-Mediating Effect of Interpersonal Competence and Social Support on the Relationship between ADHD Traits and Depression in Early Adulthood
Authors: An Jae Sun
Published: March 2023

Background: This study aims to analyze the relationship between interpersonal competence, social support, ADHD traits, and depression in early adulthood, and by using the double-mediation model, to examine the effect of the first two variables on the last two.Methods: The participants comprised 146 male and 155 female adults aged between 19 and 34, from whom data was collected through the Korean version of the WHO Adult ADHD Self-Report Scale, interpersonal competence and depression subscales of the mental health test developed by Suh et al., and social support scale developed by Iverson et al.Results: The results showed that adult ADHD traits were negatively correlated with interpersonal competence and social support, and positively correlated with depression. In addition, interpersonal competence was positively correlated with social support and negatively correlated with depression, whereas social support was negatively correlated with depression. Finally, interpersonal competence and social support sequentially mediated adult ADHD traits and depression.Conclusions: This sequential double-mediating effect is of great academic significance and suggests that improving interpersonal competence and social support may be effective in mitigating the negative effect of ADHD traits on depression in early adulthood.
Celebrating a Storied History: Moscow Preobrazhenskaya Mental Hospital Marks its 245th Anniversary
Authors: Burygina Larisa A. , Golubev Sergey A. , Filipchenko Oksana V.
Published: March 2023

In 2022, Mental-health Clinic No. 4 named after P. B. Gannushkin, one of the oldest mental health institutions in Russia known as Preobrazhenskaya Hospital before the October Revolution of 1917, celebrated its 245th anniversary. The history of the hospital reflects all stages of the evolution of the basic principles and aspects of mental health care in Russia. On many occasions, the institution served as a platform for eminent researchers and clinicians to achieve scientific breakthroughs and their application in practice. This article is a review of the major milestones in the history of the hospital. It highlights the great achievements of its psychiatrists and presents some previously unpublished archival documents that offer a new perspective on the history of Preobrazhenskaya Hospital.
Modern Approaches to the Diagnosis of Cognitive Impairment and Alzheimer’s Disease: A Narrative Literature Review
Authors: Ochneva Aleksandra G. , Soloveva Kristina P. , Savenkova Valeria I. , Ikonnikova Anna Yuryevna , Gryadunov Dmitriy A. , Andryuschenko Alisa V.
Published: March 2023

BACKGROUND: The aging of the worlds population leads to an increase in the prevalence of age-related diseases, including cognitive impairment. At the stage of dementia, therapeutic interventions become usually ineffective. Therefore, researchers and clinical practitioners today are looking for methods that allow for early diagnosis of cognitive impairment, including techniques that are based on the use of biological markers.
AIM: The aim of this literature review is to delve into scientific papers that are centered on modern laboratory tests for Alzheimers disease, including tests for biological markers at the early stages of cognitive impairment.
METHODS: The authors have carried out a descriptive review of scientific papers published from 2015 to 2023. Studies that are included in the PubMed and Web of Science electronic databases were analyzed. A descriptive analysis was used to summarized the gleaned information.
RESULTS: Blood and cerebrospinal fluid (CSF) biomarkers, as well as the advantages and disadvantages of their use, are reviewed. The most promising neurotrophic, neuroinflammatory, and genetic markers, including polygenic risk models, are also discussed.
CONCLUSION: The use of biomarkers in clinical practice will contribute to the early diagnosis of cognitive impairment associated with Alzheimers disease. Genetic screening tests can improve the detection threshold of preclinical abnormalities in the absence of obvious symptoms of cognitive decline. The active use of biomarkers in clinical practice, in combination with genetic screening for the early diagnosis of cognitive impairment in Alzheimers disease, can improve the timeliness and effectiveness of medical interventions.
Monetizing of ethics in the digital economy
Authors: Gurov Oleg
Published: March 2023

This article examines the role of ethics and morality for businesses and consumers in the digital economy. The authors argue that companies that adhere to ethical principles build a positive reputation and gain customer trust. The paper describes various approaches to monetizing ethics and morality in the digital economy, such as creating ethical products and services, embedding ethical practices in business operations, and promoting social responsibility. The authors note, however, that incorporating ethical categories into business planning and practice is challenging for several reasons, such as the rapidly changing technological landscape, unresolved legal issues, inertia in decision makers' thinking, and others. The authors predict that in the future the integration of ethical and moral principles into business will be driven by increased regulation and changing consumer behavior. The authors draw attention to the need for further research and observation in this area to understand the processes taking place and to form a set of tools for the effective promotion of ethical practices and social responsibility in the digital economy.
Resenha sobre o e-Book APS Forte no SUS - no combate à pandemia de Covid-19
Authors: Dias Monique Nunes Fiuza
Published: March 2023

Tendo como ponto de partida a emergência sanitária representada pela Covid-19, bem como o seu caráter pandêmico, os profissionais de saúde atuantes na Atenção Primária à Saúde (APS), onde o vínculo e o contato próximo ao usuário caracterizam fortemente esse nível de atenção, remodelaram o processo de trabalho através de inovações implantadas de forma emergencial. Assim, a iniciativa “APS Forte no SUS – no combate à pandemia de Covid-19” (2020), é uma obra publicada pela Organização Pan-Americana da Saúde (OPAS) e Ministério da Saúde, com o objetivo de reunir e difundir as experiências exitosas de todo o País. Entre elas merecem destaque: a inserção digital, a adaptação da atenção primária à saúde às diferentes vulnerabilidades regionais, o fortalecimento das ações intersetoriais, de vigilância, monitoramento, educação em saúde e comunicação, bem como a contribuição de cada experiência para o fortalecimento do Sistema Único de Saúde (SUS) neste cenário.
Relato de experiência do Coletivo de Residentes do Programa de Gestão em Saúde: a importância de espaços coletivos na formação em serviço
Authors: Lirio Camilo de Oliveira , Carvalho Thayná de Oliveira , Gualdi Carolina Brandt , Strohschoen Maria Tereza Blanco , Blankenheim Ana Paula
Published: March 2023

O Programa de Gestão em Saúde da Residência Integrada Multiprofissional da Escola de Saúde Pública do Rio Grande do Sul foi criado em 2020. Em virtude da necessidade de concretizar o projeto transversal previsto no Projeto Pedagógico e de promover a integração entre os residentes, surge o Coletivo de Residentes do Programa de Gestão em Saúde. O presente trabalho objetiva relatar o processo de criação do Coletivo, enquanto componente curricular proposto pelos residentes, bem como a sua atuação e importância. Para tanto, foi realizado um relato de experiência de residentes do primeiro e do último ano (que ingressaram na primeira turma), aliado a uma pesquisa documental dos materiais produzidos pelo Coletivo. Os resultados indicam que o Coletivo proporcionou o desenvolvimento de atividades que qualificam a formação dos futuros gestores e o próprio Programa, em consonância com os princípios do Sistema Único de Saúde.
Consultório de enfermagem no Centro POP: uma experiência inovadora em parceria com o Consultório na Rua
Authors: Campos Fagner Alfredo Ardisson Cirino , Ventura Carla Aparecida Arena
Published: March 2023

A População em Situação de Rua (PSR) é um grupo social marginalizado e discriminado, que tem pouco acesso aos direitos sociais, principalmente aos de saúde. No cuidado à PSR, o enfermeiro tem papel fundamental ao favorecer o acesso aos dispositivos que podem possibilitar a resolução de seus problemas e satisfação das suas necessidades individuais e coletivas. O objetivo deste trabalho é relatar como aconteceu a instalação de um Consultório de Enfermagem (CE) em um serviço de assistência social (Centro POP) para atendimento especializado em saúde à PSR que frequentava esse ambiente, entre os anos de 2020 e 2021. Para tanto, foi instalado um CE no Centro POP, com apoio da Secretaria Municipal de Saúde, visto que neste local se concentra maior quantitativo de PSR. Essa ação teve como intuito proporcionar o máximo acesso aos cuidados de saúde à PSR, sendo oferecidos cuidados de consultas de enfermagem, testagem rápida para doenças sexuais, curativos, orientações e demais procedimentos de enfermagem necessários a fim de promover a dignidade humana à PSR.
Desafios e potencialidades para a estruturação e o funcionamento dos Conselhos Locais de Saúde de Porto Alegre
Authors: Machado Frederico Viana , Dowbor Monika Weronika , Pinto Rodrigo Silveira , Pedroso Vitória D’Avila , Senna Franciele Santiago Ferreira , Oliveira Alexandra Freiberger Kowalczyk de , Tarouco Mariana Lenz
Published: March 2023

Resumo: Introdução: O objetivo deste trabalho foi identificar a presença, bem como formas de atuação e funcionamento dos Conselhos Locais de Saúde (CLS) da cidade de Porto Alegre, visto o seu fomento como meta de gestão neste município, incluída por iniciativa do Conselho Municipal de Saúde. Metodologia: Trata-se de uma pesquisa exploratória e descritiva. Primeiramente, determinou-se a quantidade de CLS ativos. Em seguida, procurou-se agendar uma entrevista semiestruturada em grupo com os CLS encontrados, utilizando um instrumento desenvolvido e validado. Foi feita a estatística descritiva com a determinação de frequências. Resultados e Discussão: Foram encontrados 56 CLS ativos e 32 entrevistas foram realizadas. Os CLS têm uma Coordenação Local instituída e presente, com reuniões ocorrendo com pautas definidas, onde são discutidos assuntos de saúde e da vida comunitária, ainda que com baixa participação da comunidade. Os CLS usam a estrutura descentralizada de conselhos bem como outros canais como sistema de justiça e representantes políticos para encaminhar as demandas. Considerações finais: A inclusão de CLS como meta de gestão mostrou um resultado positivo no aumento dos CLS. Porém se deve pensar mais em cada território e suas particularidades, com ações que diversifiquem as formas de mobilização.
Floristic zoning of high-mountain landscapes of the Central and North-Eastern Caucasus
Authors: Astamirova M A. , Taysumov M. A. , Abdurzakova A. S. , Magomadova R. S. , Omarkhadzhieva F. S.
Published: March 2023

Aim. To carry out floristic zoning of high-mountain landscapes of the Central and North-Eastern Caucasus.

Material and Methods. A list of obligate petrophytes was compiled based on the results of processing the authors' own field collections and taking into account information from floristic reports and determinants. The assessment of the similarity of species composition of the flora of the high-mountain landscapes of the Central and North-Eastern Caucasus was carried out according to the Jaccard and S0rensen-Chekanovskiy coefficients.

Results. Based on the analysis of similarity of floristic lists, the geographical boundaries were corrected and three floristic regions of petrophilic high-mountain flora were identified.

Conclusion. This analysis of the similarity of the petrophilic floras of the high-mountain landscapes of the Central and North-Eastern Caucasus has shown that it is necessary to correct the boundaries of the existing scheme of floristic zoning, since the similarity of coefficients of floristic lists indicates the possibility of combining a number of areas based on a small degree of difference between their floras and the presence of a significant number of species whose ranges are unifying.
Distribution of orchids in the upper basin of the Ural River and questions of nature conservation
Authors: Suyundukov I. V. , Kildiyarova G. N.
Published: March 2023

Aim. Conduct a botanical and geographical analysis of the species of the fam. Orchidaceae in the upper part of the Ural River basin and provide recommendations on the organization of specially protected natural areas.

Material and Methods. The study of the distribution of orchids was carried out both on the basis of the results of our own field expeditions and on literature and herbarium collections stored in local and central herbaria (UFA, SWER, LE, MW). The coenopopulation studies were carried out in accordance with generally accepted methods.

Results. The species composition of orchids was revealed and their arealogical, ecological-coenotic, coenopopulation analysis was undertaken in the upper part of the Ural River basin. Despite the wide range, most species of the fam. Orchidaceae are characterised by a narrow ecological-coenotic confinement. The low occurrence of orchids in the conditions of the steppe and forest-steppe zones of the territories under consideration is associated with both weather-climatic and anthropogenic impacts. The coenopopulations of most species are small and the ontogenetic spectra are incomplete.

Conclusions. Species of the family Orchidaceae do not grow in economically developed territories. The remaining locations of orchids are mainly associated with landscapes that are difficult to access for anthropogenic impact. In order to preserve the steppe and forest-steppe landscapes of the Urals it is necessary to further develop the system of specially protected natural areas. The most promising for this purpose are the Krykty and Irendyk ranges, which are distinguished by their unique landscape and biological diversity.
Investigation of the inhibitory activity of extracts, fractions and secondary metabolites of Silene spp. (Caryophyllaceae) and Serratula cupuliformis (Asteraceae) on the replication of SARS-CoV-2 coronavirus
Authors: Kazachinskaia E. I. , Zibareva L. N. , Filonenko E. S. , Ivanova A. V. , Gadzhieva M. M. , Bekshokov K. K. , Kononova Yu. V. , Chepurnov A. A. , Shestopalov A. М.
Published: March 2023

Aim. In vitro analysis of the inhibitory activity of extracts, fractions and secondary metabolites of plants of the genus Silene [Caryophylaceae] and Serratula cupuliformis [Asteraceae) on the replication of SARS-CoV-2.

Material and Methods. Silene spp. and Serratula cupuliformis of the Siberian Botanical Garden of National Research Tomsk State University were used. Ethanol extracts and butanol fractions of Silene spp. were prepared. The flavonoid shaftoside and the ecdysteroid 20-hydroxyecdysone from Lychnis chalcedonica were isolated. Analysis of BAS was carried out by the HPLC method. In vitro analysis of the inhibitory activity of extracts on SARS-CoV-2 replication was performed in Vero cell culture by direct inactivation [neutralization) of virions. Comparison samples were dry ethanol extracts of chaga [Inonotus obliquus, Basidiomycota), spices of cloves [Syzygium aromaticum, Myrtaceae) and root of licorice [Glycyrrhiza glabra L., Fabaceae).

Results. The inhibitory activity of ethanol extracts and butanol fractions of Silene spp., as well as individual compounds [shaftozide and 20-E) was revealed in the range of 50% effective concentrations [EC50) when dissolved in water from 339.85±83.92 pg/ml to 1.59±0.39 pg/ml and when dissolved in DMSO from 119.34±26.34 pg/ml to 2.22±0.57 pg/ml, respectively. The butanol fraction of Serratula cupuliformis was active with EC50=21.74±4.80 and 27.42±6.05 pg/mL. These results for some samples of Silene spp. and Serratula cupuliformis are comparable to the EC50 values of the comparators.

Conclusion. The results obtained suggest the presence of biologically active substances in the herbal preparations studied that act destructively on virions of SARS-CoV-2 and affect one of the main stages of its "life" cycle - on the attachment to receptors of sensitive cells.
La recepción del quipu en el siglo XVI
Authors: Brokaw Galen
Published: March 2023

En este ensayo me propongo demostrar que el número reducido de referencias a los quipus después de 1583 no se debe a tales razones: la prohibición atribuida al Tercer Concilio no fue universal y la administración colonial nunca rechazó el quipu como medio fidedigno. En general, los oficiales españoles no se preocupaban mucho por el quipu en sí. Se enfocaban, en realidad, en la autenticidad de la información más que en la legitimidad del medio material. La función del quipu dentro de la administración colonial cambia a través del siglo XVI, pero la visibilidad reducida del quipu resulta de la disminución de las actividades administrativas que requerían el uso de informantes indígenas y no de una campaña de extirpación. Es cierto que el quipu empieza a caer en desuso en muchas partes de los Andes durante el período colonial, pero este es un proceso gradual que resulta de la asimilación, la aculturación y la dominación cultural y política.
El descargo en las cuentas de quipucamayos en un pleito entre encomenderos (Huamanga, 1572)
Authors: Medelius Mónica
Published: March 2023

El análisis que ahora presento es especialmente relevante para conocer cómo las autoridades indígenas caciques y quipucamayos podrían introducir los cambios en quipus por motivos tales como a) la tripartición de una tasa inicial impuesta para ordenar el pago de tributos; b) la monetización exigida en la entrega de tributos; y c) la sustitución de los productos solicitados y efectivamente entregados por circunstancias que se expusieron cuando se retomó el pleito en 1572. En el presente artículo quisiera hacer énfasis en cómo los caciques y quipucamayos hicieron frente a la monetización de los bienes que debían listar en sus quipus. Se trata de un análisis exploratorio que merece mayor atención y comparación con otras situaciones que se presentaron en esta misma época. Las modificaciones que los caciques y principales hicieron en los quipus muestran el dominio que tenían de este instrumento, lo cual no es otra cosa que el reflejo de la autoridad que aún conservaban en la administración colonial.
Digitalization processes in production, public administration, and the social sphere in Russia
Authors: Ruban Larissa , Аnanjin Maksim
Published: March 2023

The article presents materials that reveal the urgent need of modernity, namely: the modernization of the Russian economy, the system of public administration and the social sphere with the help of digitalization, substantiates and shows the possibilities of introducing innovations in all spheres of life of the Russian Federation. The authors use the results of domestic and foreign (Ernst & Young, KMDA, etc.) sociological research (expert surveys in the form of interviews, included observation, content analysis of scientific publications and information presented in the media). The methodology of the study is a system analysis that allows a comprehensive and in-depth study of the stated problem. A comparative analysis of the course of the digitalization process in our country and abroad allows us to compare the achievements of Russian and foreign specialists, to identify the most common problems and risks associated with innovation. Also, the authors, using the Sorbonne method of in-depth study and analysis of scientific and informational texts, carry out painstaking work to create a dynamic picture of digitalization of all sectors of Russian society and identify promising areas and opportunities for fruitful cooperation between Russia and foreign countries that have achieved the greatest results in this area. The need to intensify modernization processes in our country is connected with the aggravation of the external situation and the introduction of new packages of tough anti-Russian sanctions by the United States and the European Union after the start of Russia's special operation in Ukraine on February 24, 2022. The collective West seeks to contribute to the isolation of our country, inflict the strongest damage to the Russian Federation and counteract the use of new foreign high technologies and imported equipment.
Civil society institutions in the formation of the national identity of young people
Authors: Averkieva Ekaterina
Published: March 2023

Сardinal civilizational transformations, changes in geopolitics and international relations are taking place. Under these conditions, the formation of the national identity of citizens plays an important role in ensuring the stability of the development of the state. The analysis carried out shows that a significant part of Russian young people has insufficiently formed national identity, willingness to work for the common good and prosperity of the Motherland. In recent years, the state has paid special attention to the formation of the national identity of young people. There is also a noticeable increase in the involvement of civil society in the political life of the country, but for the effective work of civil society institutions in the formation of national identity, the political socialization of Russian youth, there are not enough methodological developments, recommendations and technological materials. The autonomous non-profit organization "Innovative Center for the Development and Education of Children and Youth" has developed a complex Model of the political socialization of Russian youth by the institutions of civil society, which includes detailed theoretical and practice-oriented parts and is designed to become a key methodological material in the field of socio-patriotic work with youth of non-profit civil organizations. organizations. The developed Model has been successfully tested for six years on the example of ten All-Russian Internet contests of a patriotic orientation and can be recommended for use in the work of a wide range of Russian civil society institutions.
Affecting Factors for Dietary, Exercise and Behavioral Modification of Women on Diet Improvement through Mediating Effects of Improve the Quality of Life
Authors: Oh Yoojin
Published: March 2023

Purpose: This study examined the factors affecting dietary, exercise, and behavioral modification of women on diet improvement using mediating effects of improving the quality of life.Methods: To conduct this study, a survey was conducted on female clients with obesity in eight franchise body-type management rooms in May 2022. Consequently, out of the 318 copies of the questionnaire, 275 copies were used for analysis, excluding false answers. The analysis of this study was conducted using Predictive Analytics SoftWare Statistics by Statistical Package for the Social Sciences (SPSS) 19.0 and AMOS 19.0. First, frequency analysis was conducted to analyze the general characteristics of the study subjects, and factor analysis was conducted using Varimax rotation to derive variables as a dimension of factors. Second, reliability analysis was conducted to calculate the reliability coefficient (Cronbach's α). Third, confirmatory factor analysis was conducted to analyze the reliability and validity of the model, and model suitability was confirmed using RMSEA, RMR, GFI, NFI, TLI, and CFI values. Fourth, structural model analysis was conducted for the process of confirming the research problem, and lastly, Sobel verification of the mediating effect was conducted.Results: The results of Sobel verification for the intermediate path were as follows: Recognition affecting diet improvement mediating the improved quality of life is 3.017 (p<0.05); attitude is 2.691 (p<0.05); action is 0.997 (p<0.05); durability is 1.607 (p<0.001); and effectiveness is 0.961 (p<0.001), respectively.Conclusion: It is vital to analyze the current status and needs of people with obesity’s diet, exercise, and behavior modification, as well as to supplement them using various devices to conduct the set living rules properly. Thus, attention and efforts from the government, private sector, schools, and local governments are needed to provide conditions for improving the quality of life and continuous diet.
The Histopathological Features of Syphilis and Its Mimickers
Authors: Purnamasari Indah , Hidayati Afif Nurul , Etty Hary Kusumastuti  , Isaak Effendy
Published: March 2023

Highlights:

The importance of having a strong suspicion for syphilis and maintaining close contact between dermatologists and pathologists cannot be understated.
Understanding the clinical relationship and histopathological features of syphilis is crucial for accurate diagnosis and distinction from its histopathologic mimickers.

 
Abstract:
Settings
Syphilis, also known as "the great imitator," is a sexually transmitted infection with a variety of clinical symptoms and histopathological similarities to other infectious diseases. Public health concerns about syphilis have grown significantly. Since 2000, there has been an increase in syphilis prevalence in the United States, with a 17.6% increase from 2015 to 2016. From 2000 to 2019, the number of syphilis cases throughout Asia increased from 0.9% to 30.9%, whilst the number of cases in Indonesia decreased from 22.5% to 14.4%. Specific serological tests for syphilis can usually detect and confirm the diagnosis and offer follow-up care in most cases. However, in certain instances, the clinical characteristics discovered during testing can be identical to those of other diseases, which may lead to inconsistent diagnosis. Considering that the diagnostic pathology is pertinent to the clinical circumstances, a histopathological investigation may be useful for differentiating syphilis mimickers. Pathology is essential for identifying potential syphilis patients with ambiguous clinical symptoms. This study’s purpose was to assist dermatologists and pathologists in identifying "mimickers" that require a biopsy and in determining the correct diagnosis and treatment coutsed based on etiology.
Effects of spirulina (Arthrospira maxima) on teratogenicity and diclofenac-induced oxidative damage in Xenopus laevis
Authors: Pérez-Alvarez Itzayana , Islas-Flores Hariz , Sánchez-Aceves Livier Mireya , Gómez-Olivan Leobardo Manuel , Chamorro-Cevallos Germán
Published: March 2023

Diclofenac (DCF) is a medication that is highly consumed and eliminated worldwide; it is constantly detected in the environment (primarily in water) and resists conventional degradation processes. It was included in the European Union watch list for the water framework. There are no regulations for this compound in Mexico. Therefore, this study evaluated the protective effect antioxidant activity of spirulina (Arthrospira maxima) against DCF-induced toxicity in Xenopus laevis at early life stages. X. laevis oocytes were exposed at the medium blastula stage for 96 h to three different mixtures: DCF+S 2 (149 µg L-1 DCF plus 2 mg L-1 spirulina), DCF+S 4 (149 µg L-1 DCF plus 4 mg L-1 spirulina), DCF+S 10 (149 µg L-1 DCF plus 10 mg L-1 spirulina). Other groups of oocytes were also exposed to DCF 149 µg L-1 and a control group. The mortality and malformation rate, growth, lipid peroxidation, and antioxidant enzymatic activity (superoxide dismutase and catalase) were determined. Spirulina at 4 and 10 mg L-1 reduced DCF-induced mortality by 80% and reduced malformations in severity and frequency. The abnormalities were malformations of the eye, tail, notochord, intestine, and rectum. All spirulina exposure groups showed an increase in total body size compared to those exposed to DCF.
High Effectiveness of a Smoking Cessation Program During the COVID-19 Pandemic
Authors: Kruk Aleksandra , Czerwińska Celina , Dolna-Michno Justyna , Broniatowska Elżbieta , Kolanko Emanuel
Published: March 2023

The coronavirus disease–2019 pandemic created major obstacles for effective smoking cessation programs by significantly limiting access to healthcare. This retrospective study aimed to assess the effectiveness of a self-developed smoking cessation program during the pandemic. The program was based on remote lectures, educational interventions, and hybrid services provided by an outpatient clinic. We enrolled 513 participants between January 2019 and February 2022. Data on demographic characteristics, medical history, and smoking status at baseline and after at least 1-year follow-up were collected from medical records and a standardized self-developed questionnaire. Participants were classified into two groups according to their current smoking status. The smoking cessation rate at 1 year was 37%. Major predictors of smoking cessation were the place of residence, ability to refrain from smoking during severe illness, and the number of cigarettes smoked per day. The proportion of participants with high levels of nicotine dependence at baseline was 40.8% vs 29.1% at 1 year (p=0.003). In the group that did not quit smoking, there were more participants who smoked within 5 minutes after waking up, as compared with participants who quitted smoking (40.4% vs 25.4%; p=0.0004). Effective smoking cessation interventions can be performed using remote counseling and education.
The Influence of Soil Nutrients Availability on Banana Bunchy Top Disease Incidence in Banyumas Regency, Central Java Province, Indonesia
Authors: Kurniawan Ruly Eko Kusuma , Rahayuniati Ruth Feti , Nurtiati Nurtiati
Published: March 2023

The banana bunchy top is one of the important diseases on bananas, presumably coupled with the influence of soil properties, despite no fixed information regarding their relationship. Therefore, this study aims to map the soil nutrient contents at some banana plantation center and determine the effect of nutrient availability on the incidence of banana bunchy top disease (BBTD). This study set a purposive sampling according to the banana cultivation distribution to gain soil samples and data on BBTD incidence. Soil samples were analyzed for macronutrient content, including N capacity using the Kjeldahl method, Bray method for P analysis, and CH4OAc methods for K analysis. According to the investigation, the range of total N was 0.2% to 0.75%, showing BBTD incidence from 5.8% to 9.47%, respectively. In line with the total P, BBTD incidence increased from 8.03% to 9.62% in the P content of 15 to 35 ppm. In contrast, in the total K of 0.5 to 1 cmol(+) kg-1, the BBTD incidence tended to decline from 9.68% to 9%. It was concluded that BBTD incidence would increase with the higher levels of N and P but decrease with the higher K. In Banyumas Regency, BBTD incidence increased in the altitude range of 100 to 300 m above sea level, then decreased at a higher altitude. BBTD incidence also exhibited an unstable response to pH changes. Bunchy top disease incidence was the highest between pH 5 and 8. This could be a guide to soil management to reduce BBTD incidences.
Trap Culture and Colonization of Arbuscular Mycorrhizal Fungi from Corn Roots in Tidal Swamps Using Several Host Plants
Authors: Sefrila Marlin , Ghulamahdi Munif , Purwono Purwono , Melati Maya , Mansur Irdika
Published: March 2023

Arbuscular mycorrhizal fungi (AMF) are mycorrhizal from the endomycorrhizal group. The fungi live on higher plants' roots by performing symbiotic mutualism. This study aimed to identify AMF spores after trapping in corn roots and the degree of root infection by AMF in several host plants. The study was conducted using tidal swamps soil samples taken from Mulyasari Tanjung Lago Village, Banyuasin Regency, South Sumatra. The experiment used a randomized block design and three replicates with four host plants as a treatment: corn, soybean, sugarcane (monoculture) and sugarcane-soybean (combination). The results showed that AMF spores found on corn roots after trapping were from the Acaulospora sp. and Glomus sp. groups. Hyphae, vesicles, arbuscules and spores are AMF structures found in the roots of host plants infected by AMF. The percentage of AMF infection in host plant roots ranged from 1.11% to 77.44% where the highest was in maize host plant roots at 77.44% in the form of internal hyphae. The maize host plant has a high potential to be colonized by AMF compared to a mixture of soybean, sugarcane and sugarcane-soybean.
Mechanical Properties Of Pack Carburized AISI 4340 With Variation Energizer Composition of Barium Carbonate (BaCO3) And Sodium Carbonate (Na2CO3)
Authors: Setiawan Agris , Yusmania Sapna , Sudiyanto Anton
Published: March 2023

The aim of this research was to determine the mechanical strength of AISI 4340 steel after pack carburizing with variation of barium carbonate (BaCO3) and sodium carbonate (Na2CO3) as energizer. The various ratios of energizer: 40/60, 50/50 and 60/40 w/w%. Mechanical test was conducted to determine tensile (ASTM E-8), impact charpy strength (ASTM E-23), rockwell hardness number (ASTM E-18) and microstructure characterization (ASTM E-3). Both type specimens were temperature pack carburized is 950ºC and holding time is 3 hours.  Results showed that specimen C has a lowest ultimate tensile strength mean values than the other specimens, which is 333.43±30.22 MPa. The results of the impact test showed that the lowest impact energy value is found in specimen C, which is 4.32 joules and the highest impact energy value is found in specimens without treatment, which is 15.80 joules. Based on microscope observation indicated that microstructure of specimen was martensite structure increase and the results of the hardness test was influenced by martensite phase, specimen C has the highest hardness compared to other specimens, which is 80.70 HRC while the untreated specimen is 56.90 HRC.
The Anticipated Nankai Trough Earthquake and Tsunami in Japan: Determinant Factors of Residents’ Pre-Event Evacuation Intentions
Authors: Hirano Kanan , Fukushima Yo , Maruya Hiroaki , Kido Motoyuki , Sugiura Motoaki
Published: March 2023

As a countermeasure against M8–9 class Nankai Trough earthquakes, the Japan Meteorological Agency started a service to release “Nankai Trough Earthquake Extra Information (Megathrust Earthquake Alert).” This alert is released after an M8.0 or higher earthquake occurs and the possibility of a subsequent earthquake is evaluated to be higher than usual. This is an innovative attempt at disaster mitigation in Japan as it encourages residents in the predefined area to pre-evacuate for one week when tsunami risk is higher. However, the factors influencing the evacuation behavior of residents are unknown. In this study, we investigated factors contributing to residents’ pre-event evacuation intentions using the hierarchical multiple regression analysis. We focused on the extent to which the recognition of the hazards and risks of the Nankai Trough earthquake and the response to the Extra Information, which are changeable by the local governments’ public relations activities, contributed to pre-event evacuation intentions after controlling for disaster-related general attitude and sociodemographic factors. Further, we paid special attention to residents’ degree of recognition of this information by checking the accuracy of their understanding of whether they lived within the pre-event evacuation area. The results showed that the recognition factors were relevant but less so than the general attitude toward disaster and more so than the sociodemographic factors. In addition, residents’ recognition accuracy was low. Our results suggest that it is important for local governments to make adequate efforts to encourage residents to evacuate.
Terahertz techniques for better hazelnut quality
Authors: Greco Manuel , Leccese Fabio , Giovenale Emilio , Doria Andrea
Published: March 2023

In recent years, technological innovation has acquired a fundamental role in the agri-food sector and in particular in food quality control. The development of technology allowed to improve the quality of the food before it is placed on the market. Recently, non-invasive techniques such as those operating in the THz spectral band were applied to the field of food quality control.

In the laboratories of the ENEA centre in Frascati, close to Rome-Italy, has been developed a THz imaging system operating in reflection mode and an experimental setup able to measure both reflection and transmission of the samples in the frequency range between 18-40 GHz. With these two setups will distinguish rotten and healthy hazelnuts acquiring in real time both images of the fruit inside the shell by using the imaging system and the transmission data exploiting the 18-40 GHz system.
Lamprophyres of the Southern Coast of Novaya Zemlya: Composition, Tectonomagmatic Position, and New Age Data
Authors: Korago E.A. , Stolbov N.M. , Burnaeva M.Yu. , Vasil’eva I.M.
Published: March 2023

—The Novaya Zemlya Archipelago is an early Cimmerian folded structure located on the northern periphery of the Ural–Mongolian fold belt. The geologic development of the archipelago is well-studied and serves as a benchmark for interpolating its features to the shelf of the surrounding water areas, whose sedimentary cover contains large accumulations of hydrocarbon raw materials. In addition to the stratified section of essentially sedimentary rocks, it is of interest to study igneous formations, which clearly mark tectonic events in the geologic history of the archipelago. In the light of this, the age of these formations remains important. The age of some magmatic complexes of the archipelago is still debatable. This paper focuses upon a lamprophyre complex in the extreme south of Novaya Zemlya, which has been dated at the Late Proterozoic or Late Proterozoic–early Paleozoic(?) until recently. The results obtained for the U–Pb age of apatites contained in the rocks of this complex confirm the Devonian age of the lamprophyres, and it is suggested by estimates based on the presented data that the emplacement time lies in a range from 360 to 398 Ma. This means that they might be associated with riftogenic processes on Novaya Zemlya since the second half of the Early Devonian, when the stage of short-term stable carbonate sedimentation (in the first half of the Devonian Period) was replaced by the initiation of a new structural–formational zonal sequence.
Antiviral Response and Immunopathogenesis of Interleukin 27 in COVID-19
Authors: Valdés-López Juan Felipe , Urcuqui-Inchima Silvio
Published: March 2023

The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, is associated with a high mortality rate. The clinical course is attributed to the severity of pneumonia and systemic complications. In COVID-19 patients and murine models of SARS-CoV-2 infection, the disease may be accompanied by over-exuberant production of cytokines, leading to accumulation of immune cells in affected organs such as lungs. Previous reports have shown that SARS-CoV-2 infection antagonizes interferon (IFN)-dependent antiviral response, thereby preventing the expression of IFN-stimulated genes (ISGs). Lower IFN levels have been linked to more severe COVID-19. Interleukin 27 (IL27) is a heterodimeric cytokine composed of IL27p28 and EBI3 subunits that induce both pro- and anti-inflammatory responses. Recently, we and others have reported that IL27 also induces a strong antiviral response in an IFN-independent manner. Here, we investigated transcription levels of both IL27 subunits in COVID-19 patients. Results show that SARS-CoV-2 infection modulates TLR1/2-MyD88 signaling in PBMCs and monocytes, and induces NF-κB activation and robust pro-inflammatory response-dependent NF-κB-target genes expression, including EBI3; as well as it activates IRF1 signaling, that induces IL27p28 mRNA expression. Results suggest that IL27 induces a robust STAT1-dependent pro-inflammatory and antiviral response in an IFN-independent manner in COVID-derived PBMCs, and Monocytes as a function of severe COVID-19 clinical course. Similar results were observed in SARS-CoV-2 Spike protein-stimulated macrophages. Thus, IL27 can trigger host antiviral response suggesting the possibility of novel therapeutics against SARS-CoV-2 infection in humans.
A new method for the detection of Mycobacterium tuberculosis based on the CRISPR/Cas system
Authors: Zhang Xiaoyu , He Xiaoying , Zhang Yubo , Chen Lei , Pan Zhaobao , Huang Yueying , Li Heng
Published: March 2023

Object: Mycobacterium tuberculosis (MTB) is a bacterium that can cause zoonoses by aerosol transmission. Tuberculosis (TB) caused by MTB places a heavy burden on world public health security. The development of efficient, specific, convenient, and inexpensive MTB assays is important for the prevention and control of TB.
Methods: Clustered Regularly Interspersed Short Palindromic Repeats (CRISPR) is a special DNA repeat family widely present in bacterial and archaeal genomes. In this study, we established a specific detection method for MTB using the CRISPR system, combined with recombinase mediated isothermal nucleic acid amplification (RAA) to improve the sensitivity of the detection system and achieve "two-level" amplification of the detection signal. The sensitivity and specificity of RAA combined with the CRISPR/Cas system were analyzed. Using BACTEC 960 culture as the gold standard for the detection of MTB, we established the TB-CRISPR technique by testing 504 samples from patients with suspected tuberculosis.
Results: MTB H37Ra could be detected as low as 3.13 CFU/mL by the CRISPR-Cas12a system targeting IS6110. With BACTCE960 culture (120 positives and 384 negatives) as the gold standard, the sensitivity of the TB-CRISPR technique was 0.883 (0.809-0.932) and the specificity was 0.940 (0.910-0.961). According to the receiver operating characteristic (ROC) curve analysis, the area under the curve (AUC) reached 0.944 (0.914-0.975) within 95% CI. The positive likelihood ratio (PLR) was 14.747 (9.870-22.035) and the negative likelihood ratio (NLR) was 0.124 (0.076-0.203). The positive predictive value (PPV) was 0.822 (0.742-0.881) and the negative predictive value (NPV) was 0.963 (0.937-0.979).
Conclusion: TB-CRISPR realizes the rapid screening and diagnosis of MTB. The whole detection time is less than 1.5h. It is easy to operate and does not need to rely on complex instruments. It is of great significance for the rapid detection of MTB and the clinical diagnosis of TB.