bluuebunny/crossref_metadata_2025 huggingface dataset:
Green Silver Nanoparticles Promote Inflammation Shutdown in Human Leukemic Monocytes
Authors: Cascione Mariafrancesca , Rizzello Loris , Manno Daniela , Serra Antonio , De Matteis Valeria
Published: January 2022

The use of silver nanoparticles (Ag NPs) in the biomedical field deserves a mindful analysis of the possible inflammatory response which could limit their use in the clinic. Despite the anti-cancer properties of Ag NPs having been widely demonstrated, there are still few studies concerning their involvement in the activation of specific inflammatory pathways. The inflammatory outcome depends on the synthetic route used in the NPs production, in which toxic reagents are employed. In this work, we compared two types of Ag NPs, obtained by two different chemical routes: conventional synthesis using sodium citrate and a green protocol based on leaf extracts as a source of reduction and capping agents. A careful physicochemical characterization was carried out showing spherical and stable Ag NPs with an average size between 20 nm and 35 nm for conventional and green Ag NPs respectively. Then, we evaluated their ability to induce the activation of inflammation in Human Leukemic Monocytes (THP-1) differentiated into M0 macrophages using 1 µM and 2 µM NPs concentrations (corresponded to 0.1 µg/mL and 0.2 µg/mL respectively) and two-time points (24 h and 48 h). Our results showed a clear difference in Nuclear Factor κB (NF-κb) activation, Interleukins 6–8 (IL-6, IL-8) secretion, Tumor Necrosis Factor-α (TNF-α) and Cyclooxygenase-2 (COX-2) expression exerted by the two kinds of Ag NPs. Green Ag NPs were definitely tolerated by macrophages compared to conventional Ag NPs which induced the activation of all the factors mentioned above. Subsequently, the exposure of breast cancer cell line (MCF-7) to the green Ag NPs showed that they exhibited antitumor activity like the conventional ones, but surprisingly, using the MCF-10A line (not tumoral breast cells) the green Ag NPs did not cause a significant decrease in cell viability.
Correlation between the Dendritic Structure and Lattice Parameter of γ′-Phase in Single-Crystalline Turbine Blades Made of Superalloys
Authors: Krawczyk Jacek , Bogdanowicz Włodzimierz
Published: January 2022

The dendritic structure and the distribution of the γ′-phase lattice parameter (aγ′) along selected lines of the longitudinal section in a model single-crystalline blade made of CMSX-4® nickel-based superalloy were studied. It was established that there is a correlation between the value of the aγ′ and the predomination of initial or ending fragments of the secondary dendrite arms. It is most noticed for the areas where the dendrite growth conditions are similar to steady. They are located in the center and near the root’s selector extension (SE) area. The correlation has been related to the dendritic segregation mechanism. It was shown that in the single-crystalline blades obtained by the directional crystallization using a spiral selector, the “walls” of the primary dendrite arms that grow at a low angle to the blade axis are created. It was found for the first time that the value of the lattice parameter aγ′ is decreased near such “walls”. Additionally, it was found that competitive growth of the dendrites may occur at a distance of even several millimeters from the bottom surface of the root. The first-time applied X-ray diffraction measurements of aγ′ made in a single-pass along the line allow the analysis of the dendritic segregation in the whole blade cast.
An Accessible Integrated Nanoparticle in a Metallic Hole Structure for Efficient Plasmonic Applications
Authors: Devaraj Vasanthan , Choi Jong-Wan , Lee Jong-Min , Oh Jin-Woo
Published: January 2022

Addressing the severe deterioration of gap mode properties in spherical-shaped nanoparticles (NPs) becomes necessary due to their utilization in a wide range of multi-disciplinary applications. In this work, we report an integrated plasmonic nanostructure based on a spherical-shaped nanoparticle (NP) in a metallic hole as an alternative to a NP-only structure. With the help of three-dimensional (3D) electromagnetic simulations, we reveal that when a NP is positioned on the top of a metallic hole, it can exhibit superior gap-mode-based local-field intensity enhancement. The integrated nanostructure displayed a ~22-times increase in near-field enhancement characteristics, similar to cube- or disk-shaped nanostructure’s plasmonic properties. From an experimental perspective, the NP positioning on top of the metallic hole can be realized more easily, facilitating a simple fabrication meriting our design approach. In addition to the above advantages, a good geometrical tolerance (metallic hole-gap size error of ~20 nm) supported by gap mode characteristics enhances flexibility in fabrication. These combined advantages from an integrated plasmonic nanostructure can resolve spherical-shaped NP disadvantages as an individual nanostructure and enhance its utilization in multi-disciplinary applications.
Poly-Lysine Dendritic Nanocarrier to Target Epidermal Growth Factor Receptor Overexpressed Breast Cancer for Methotrexate Delivery
Authors: Narayanan Pratibha , Anitha Anju Krishnan , Ajayakumar Neethu , Kumar Kesavakurup Santhosh
Published: January 2022

A fourth generation poly-lysine dendritic nanocarrier (P4LDN)-based targeted chemotherapy for breast cancer is attempted by incorporating an epidermal growth factor receptor (EGFR)-specific short peptide E2 (ARSHVGYTGAR) and the drug methotrexate (MTX) into a nanocarrier system. The drug is incorporated into the nanocarrier using a cathepsin B cleavable spacer: glycine–phenylalanine–leucine–glycine (GFLG). The in vitro analysis of the time-dependent drug release, binding and internalization ability, and the cytotoxic nature showed that this drug delivery system (DDS) is highly effective. The efficacy analysis using non-obese diabetic/severe combined immunodeficiency (NOD-SCID) mice also showed that compared to the control group, the DDS can effectively reduce tumor volume. The mice that received the DDS appeared to gain weight more rapidly than the free drug, which suggests that the dendrimer is more easily tolerated by mice than the free drug.
Development of New Experimental Dental Enamel Resin Infiltrants—Synthesis and Characterization
Authors: Prodan Doina , Moldovan Marioara , Chisnoiu Andrea Maria , Saroși Codruța , Cuc Stanca , Filip Miuța , Gheorghe Georgiana Florentina , Chisnoiu Radu Marcel , Furtos Gabriel , Cojocaru Ileana , Delean Ada Gabriela , Cimpean Sanda Ileana
Published: January 2022

The aim of the present study was to obtain experimental infiltration materials, intended for the treatment of dental white spots, and to investigate them. Two series of infiltrants (P1–P6)/(P1F–P6F) were obtained, based on different monomer mixtures, without/with glass filler (with fluoride release ability). Each infiltrant from the second series contained the same amount of glass powder, and each infiltrant from the (P–PF) group contained the same resin composition. The characteristics of the experimental infiltrants were investigated by degree of conversion (DC), mechanical strength, water sorption (WS), and fluoride release, in addition to residual monomer for (P1F–P6F) infiltrants. The results were compared with those obtained for commercial Icon infiltrant. For the experimental infiltrants, without/with filler, the recorded DC was in the range of 58.27–89.70%/60.62–89.99%, compared with Icon (46.94%) 24 h after polymerization. The release of fluoride depends on the permeability of the polymer matrix, with respect to the water sorption, which may help to diffuse ions in the storage medium but which can also influence the release of residual monomers. The highest flexural strengths were recorded for the (TEGDMA/HEMA/Bis-GMA) infiltrants (133.94 ± 16.389 MPa/146.31 ± 7.032 MPa). The best experimental infiltrants were P2 and P2F (Bis-GMA/HEMA/TEGDMA).
Applications of Phyto-Nanotechnology for the Treatment of Neurodegenerative Disorders
Authors: Bhattacharya Tanima , Soares Giselle Amanda Borges e , Chopra Hitesh , Rahman Md. Mominur , Hasan Ziaul , Swain Shasank S. , Cavalu Simona
Published: January 2022

The strategies involved in the development of therapeutics for neurodegenerative disorders are very complex and challenging due to the existence of the blood-brain barrier (BBB), a closely spaced network of blood vessels and endothelial cells that functions to prevent the entry of unwanted substances in the brain. The emergence and advancement of nanotechnology shows favourable prospects to overcome this phenomenon. Engineered nanoparticles conjugated with drug moieties and imaging agents that have dimensions between 1 and 100 nm could potentially be used to ensure enhanced efficacy, cellular uptake, specific transport, and delivery of specific molecules to the brain, owing to their modified physico-chemical features. The conjugates of nanoparticles and medicinal plants, or their components known as nano phytomedicine, have been gaining significance lately in the development of novel neuro-therapeutics owing to their natural abundance, promising targeted delivery to the brain, and lesser potential to show adverse effects. In the present review, the promising application, and recent trends of combined nanotechnology and phytomedicine for the treatment of neurological disorders (ND) as compared to conventional therapies, have been addressed. Nanotechnology-based efforts performed in bioinformatics for early diagnosis as well as futuristic precision medicine in ND have also been discussed in the context of computational approach.
Prediction of Behaviour of Thin-Walled DED-Processed Structure: Experimental-Numerical Approach
Authors: Urbánek Miroslav , Hodek Josef , Melzer Daniel , Koukolíková Martina , Prantl Antonín , Vavřík Jaroslav , Brázda Michal , Martínek Petr , Rzepa Sylwia , Džugan Jan
Published: January 2022

Additive manufacturing (AM) becomes a more and more standard process in different fields of industry. There is still only limited knowledge of the relationship between measured material data and the overall behaviour of directed energy deposition (DED)-processed complex structures. The understanding of the structural performance, including flow curves and local damage properties of additively manufactured parts by DED, becomes increasingly important. DED can be used for creating functional surfaces, component repairing using multiple powder feeders, and creating a heterogeneous structure with defined chemical composition. For thin parts that are used with the as-deposited surface, this evaluation is even highly crucial. The main goal of the study was to predict the behaviour of thin-walled structures manufactured by the DED process under static loading by finite element analysis (FEA). Moreover, in this study, the mechanical performance of partly machined and fully machined miniaturized samples produced from the structure was compared. The structure studied in this research resembles a honeycomb shape made of austenitic stainless steel AISI 316L, which is characterized by high strength and ductility. The uncoupled damage models based on a hybrid experimental-numerical approach were used. The microstructure and hardness were examined to comprehend the structural behaviour.
Long-Term Water Balance Evaluation in Glass Ionomer Restorative Materials
Authors: Roberts Howard , Berzins David , Nicholson John
Published: January 2022

The complex role of water in glass ionomer cement (polyalkenoate) dental restorative materials has been studied, but much of the present understanding concerning water balance within these materials is based on very early studies and short-term experiments. This study evaluated the nature of the water species of six conventional and four resin modified glass ionomer restorative materials over 3 years using thermogravimetric analysis techniques. Materials were prepared, placed in crucibles, and stored in physiologic phosphate buffered saline and evaluated at 24 h, 1 week, and then at 1, 3, 6, 9, 12, 18, 24, 30 and 36 months. All materials demonstrated a significant increase in unbound water percentage content but except for the resin modified materials, the enthalpy required to remove the unbound water species did not significantly change over 36 months. Also, bound water content percentage and removal enthalpy was established at 24 h, as no significant increase was noted in both bound water content and removal enthalpy over the course of this evaluation. This study suggests that unbound water species may increase with time and is loosely held except for the resin modified materials. Protective coatings placement and re-evaluation are prudent to prevent unbound water loss.
Heat Shock Protein Overexpression-Mediated Periodontal Ligament Regeneration: A Fundamental Approach to Generate a Potential Biomaterial
Authors: Muraoka Rina , Nakano Keisuke , Kawakami Toshiyuki
Published: January 2022

The periodontal ligament (PDL) is a cell-rich fibrous connective tissue supporting the tooth roots. The tissue helps to maintain homeostasis and exhibits regenerative and repairing ability, which is mediated by the heat shock protein (HSP). Here, we experimentally created PDL tissue with notable ability to regenerate hard tissue and evaluated it as a potential biomaterial. We immunohistochemically examined the mechanical load-induced HSP overexpression in mouse PDL. Following mechanical load application and release, HSP70 localization in the PDL was altered immediately, suggesting that the HSP70 function may differ with the timing of its expression in PDL. HSP70 expressed in the cytoplasm and nucleus of fibroblasts in PDL on the tension side not only participated in periodontium repair, but also functioned as a molecular chaperone during protein expression involved in osteogenesis to restructure injured tissue. This study highlights the potential of artificially created highly functional PDL tissues as biomaterials.
Effect of the Flame Retardants and Glass Fiber on the Polyamide 66/Polyphenylene Oxide Composites
Authors: Zhang Zhenya , Yang Mingcheng , Cai Kunpeng , Chen Yang , Liu Shubo , Liu Wentao , Liu Jilin
Published: January 2022

In this work, polyamide 66/polyphenylene oxide (PA66/PPO) composites, including the flame retardants 98 wt% aluminum diethylphosphinate + 2 wt% polydimethylsiloxane (P@Si), Al(OH)3-coated red phosphorus (RP*), and glass fiber (GF), were systematically studied, respectively. The limiting oxygen index (LOI), UL-94 vertical burning level, and thermal and mechanical properties of the PA66/PPO composites were characterized. The results showed that the P@Si and RP flame retardants both improved the LOI value and UL-94 vertical burning level of the PA66/PPO composites, and PA66/PPO composites passed to the UL-94 V-0 level when the contents of P@Si and RP* flame retardants were 16 wt% and 8 wt%. On the other hand, the mechanical properties of the PA66/PPO composites were reduced from a ductile to a brittle fracture mode. The addition of GF effectively made up for these defects and improved the mechanical properties of the PA66/PPO composites containing the P@Si and RP*, but it did not change the fracture mode. P@Si and RP* flame retardants improved the thermal decomposition of PA66/PPO/GF composites and reduced the maximum mass loss rates, showing that the PA66/PPO/GF composites containing the P@Si and RP* flame retardants could be used in higher-temperature fields.
Features of Microstructure and Texture Formation of Large-Sized Blocks of C11000 Copper Produced by Electron Beam Wire-Feed Additive Technology
Authors: Osipovich Kseniya , Vorontsov Andrey , Chumaevskii Andrey , Moskvichev Evgeny , Zakharevich Ivan , Dobrovolsky Artem , Sudarikov Alexander , Zykova Anna , Rubtsov Valery , Kolubaev Evgeny
Published: January 2022

The paper investigated the possibility of obtaining large-sized blocks of C11000 copper on stainless steel substrates via electron beam wire-feed additive technology. The features of the microstructure and grain texture formation and their influence on the mechanical properties and anisotropy were revealed. A strategy of printing large-sized C11000 copper was determined, which consists of perimeter formation followed by the filling of the internal layer volume. This allows us to avoid the formation of defects in the form of drops, underflows and macrogeometry disturbances. It was found that the deposition of the first layers of C11000 copper on a steel substrate results in rapid heat dissipation and the diffusion of steel components (Fe, Cr and Ni) into the C11000 layers, which promotes the formation of equiaxed grains of size 8.94 ± 0.04 μm. As the blocks grow, directional grain growth occurs close to the <101> orientation, whose size reaches 1086.45 ± 57.13 μm. It is shown that the additive growing of large-sized C11000 copper leads to the anisotropy of mechanical properties due to non-uniform grain structure. The tensile strength in the opposite growing direction near the substrate is 394 ± 10 MPa and decreases to 249 ± 10 MPa as the C11000 blocks grows. In the growing direction, the tensile strength is 145 ± 10 MPa.
Metabolites Produced by a New Lactiplantibacillus plantarum Strain BF1-13 Isolated from Deep Seawater of Izu-Akazawa Protect the Intestinal Epithelial Barrier from the Dysfunction Induced by Hydrogen Peroxide
Authors: Diao Xiaozhen , Yamada Katsuhisa , Shibata Yuji , Imada Chiaki
Published: January 2022

This study aimed to investigate the protective effect of the metabolites produced by a new Lactiplantibacillus plantarum strain BF1-13, isolated from deep seawater (DSW), on the intestinal epithelial barrier against the dysfunction induced by hydrogen peroxide (H2O2) and to elucidate the mechanism underlying the effect. Protective effect of the metabolites by strain BF1-13 on the barrier function of the intestinal epithelial model treated with H2O2 was investigated by the transepithelial electrical resistance (TEER). The metabolites enhanced the Claudin-4 (CLDN-4) expression, including at the transcription level, indicated by immunofluorescence staining and quantitative RT-PCR. The metabolites also showed a suppression of aquaporin3 (AQP3) expression. Lactic acid (LA) produced by this strain of homofermentative lactic acid bacteria (LAB) had a similar enhancement on CLDN-4 expression. The metabolites of L. plantarum strain BF1-13 alleviated the dysfunction of intestinal epithelial barrier owing to its enhancement on the tight junctions (TJs) by LA, along with its suppression on AQP3-facilitating H2O2 intracellular invasion into Caco-2 cells. This is the first report on the enhancement of TJs by LA produced by LAB.
Anti-Larval and Anti-Algal Natural Products from Marine Microorganisms as Sources of Anti-Biofilm Agents
Authors: Wang Kai-Ling , Dou Zheng-Rong , Gong Gao-Fen , Li Hai-Feng , Jiang Bei , Xu Ying
Published: January 2022

Bacteria growing inside biofilms are more resistant to hostile environments, conventional antibiotics, and mechanical stresses than their planktonic counterparts. It is estimated that more than 80% of microbial infections in human patients are biofilm-based, and biofouling induced by the biofilms of some bacteria causes serious ecological and economic problems throughout the world. Therefore, exploring highly effective anti-biofilm compounds has become an urgent demand for the medical and marine industries. Marine microorganisms, a well-documented and prolific source of natural products, provide an array of structurally distinct secondary metabolites with diverse biological activities. However, up to date, only a handful of anti-biofilm natural products derived from marine microorganisms have been reported. Meanwhile, it is worth noting that some promising antifouling (AF) compounds from marine microbes, particularly those that inhibit settlement of fouling invertebrate larvae and algal spores, can be considered as potential anti-biofilm agents owing to the well-known knowledge of the correlations between biofilm formation and the biofouling process of fouling organisms. In this review, a total of 112 anti-biofilm, anti-larval, and anti-algal natural products from marine microbes and 26 of their synthetic analogues are highlighted from 2000 to 2021. These compounds are introduced based on their microbial origins, and then categorized into the following different structural groups: fatty acids, butenolides, terpenoids, steroids, phenols, phenyl ethers, polyketides, alkaloids, flavonoids, amines, nucleosides, and peptides. The preliminary structure-activity relationships (SAR) of some important compounds are also briefly discussed. Finally, current challenges and future research perspectives are proposed based on opinions from many previous reviews.
Biological Activity and Stability of Aeruginosamides from Cyanobacteria
Authors: Cegłowska Marta , Kwiecień Patrycja , Szubert Karolina , Brzuzan Paweł , Florczyk Maciej , Edwards Christine , Kosakowska Alicja , Mazur-Marzec Hanna
Published: January 2022

Aeruginosamides (AEGs) are classified as cyanobactins, ribosomally synthesized peptides with post-translational modifications. They have been identified in cyanobacteria of genera Microcystis, Oscillatoria, and Limnoraphis. In this work, the new data on the in vitro activities of three AEG variants, AEG A, AEG625 and AEG657, and their interactions with metabolic enzymes are reported. Two aeruginosamides, AEG625 and AEG657, decreased the viability of human breast cancer cell line T47D, but neither of the peptides was active against human liver cancer cell line Huh7. AEGs also did not change the expression of MIR92b-3p, but for AEG625, the induction of oxidative stress was observed. In the presence of a liver S9 fraction containing microsomal and cytosolic enzymes, AEG625 and AEG657 showed high stability. In the same assays, quick removal of AEG A was recorded. The peptides had mild activity against three cytochrome P450 enzymes, CYP2C9, CYP2D6 and CYP3A4, but only at the highest concentration used in the study (60 µM). The properties of AEGs, i.e., cytotoxic activity and in vitro interactions with important metabolic enzymes, form a good basis for further studies on their pharmacological potential.
Acid-Sensing Ion Channels in Glial Cells
Authors: Cegielski Victoria , Chakrabarty Rohan , Ding Shinghua , Wacker Michael J. , Monaghan-Nichols Paula , Chu Xiang-Ping
Published: January 2022

Acid-sensing ion channels (ASICs) are proton-gated cation channels and key mediators of responses to neuronal injury. ASICs exhibit unique patterns of distribution in the brain, with high expression in neurons and low expression in glial cells. While there has been a lot of focus on ASIC in neurons, less is known about the roles of ASICs in glial cells. ASIC1a is expressed in astrocytes and might contribute to synaptic transmission and long-term potentiation. In oligodendrocytes, constitutive activation of ASIC1a participates in demyelinating diseases. ASIC1a, ASIC2a, and ASIC3, found in microglial cells, could mediate the inflammatory response. Under pathological conditions, ASIC dysregulation in glial cells can contribute to disease states. For example, activation of astrocytic ASIC1a may worsen neurodegeneration and glioma staging, activation of microglial ASIC1a and ASIC2a may perpetuate ischemia and inflammation, while oligodendrocytic ASIC1a might be involved in multiple sclerosis. This review concentrates on the unique ASIC components in each of the glial cells and integrates these glial-specific ASICs with their physiological and pathological conditions. Such knowledge provides promising evidence for targeting of ASICs in individual glial cells as a therapeutic strategy for a diverse range of conditions.
A High Performance Polyacrylonitrile Composite Separator with Cellulose Acetate and Nano-Hydroxyapatite for Lithium-Ion Batteries
Authors: Chen Weiping , Wang Xiang , Liang Jianyu , Chen Yao , Ma Wei , Zhang Siyuan
Published: January 2022

The traditional commercial polyolefin separators suffer from high-temperature thermal shrinkage, low electrolyte wettability and other issues. In order to improve the overall performance of the separators, electrostatic spinning technology was applied to obtain PAN nanofiber separators with an average diameter of 320 nm. Then cellulose acetate (CA) resin and nano-hydroxyapatite (HAP) were introduced to fabricate the PAN/CA/HAP composite separators through the constant temperature hot pressing and dip-coating crafts. The composite separator has a good thermal stability, with no significant dimensional change after a constant temperature treatment of 200 °C for 35 min. The electrolyte uptake rate of the PAN/CA/HAP-1.0 composite separator reaches 281%, which exhibits an efficient ionic conductivity. At the same time, it also attains a tensile strength of 11.18 MPa, which meets the requirement for separator use. Button cells assembled from PAN/CA/HAP-1.0 composite separators have an excellent rate of performance (160.42 mAh·g−1 at 0.2 C) and cycle capability (157.6 mAh·g−1 after 50 cycles at 0.5 C). The results support that lithium-ion batteries assembled with PAN/CA/HAP-1.0 composite separators will exhibit higher safety stability and better electrochemical performance than that of polyolefin separators, with a very immense potential for application.
Quartz Crystal Microbalance Humidity Sensors Based on Structured Graphene Oxide Membranes with Magnesium Ions: Design, Mechanism and Performance
Authors: Yi Ruobing , Peng Bingquan , Zhao Yimin , Nie Dexi , Chen Liang , Zhang Lei
Published: January 2022

The application of graphene oxide (GO)-based membranes combined with a quartz crystal microbalance (QCM) as a humidity sensor has attracted great interest over the past few years. Understanding the influence of the structure of the GO membrane (GOM) on the adsorption/desorption of water molecules and the transport mechanism of water molecules in the membrane is crucial for development of applications using GOM-based humidity sensors. In this paper, by investigating the effects of oxygen-containing groups, flake size and interlayer spacing on the performance of humidity sensing, it was found that humidity-sensing performance could be improved by rational membrane-structure design and the introduction of magnesium ions, which can expand the interlayer spacing. Therefore, a novel HGO&GO&Mg2+ structure prepared by uniformly doping magnesium ions into GO&HGO thin composite membranes was designed for humidity sensing from 11.3% RH to 97.3% RH. The corresponding sensor exhibits a greatly improved humidity sensitivity (~34.3 Hz/%RH) compared with the original pure GO-based QCM sensor (~4.0 Hz/%RH). In addition, the sensor exhibits rapid response/recovery times (7 s/6 s), low hysteresis (~3.2%), excellent repeatability and good stability. This research is conducive to understanding the mechanism of GOM-based humidity sensors. Owing to its good humidity-sensing properties, the HGO&GO&Mg2+ membrane-based QCM humidity sensor is a good candidate for humidity sensing.
Microstructure and Indentation Properties of Single-Roll and Twin-Roll Casting of a Quasicrystal-Forming Al-Mn-Cu-Be Alloy
Authors: Zupanič Franc , Macerl Matjaž , Haga Toshio , Bončina Tonica
Published: January 2022

In this investigation, strips of an experimental Al-Mn-Cu-Be alloy were manufactured by high-speed single-roll and twin-roll casting to stimulate the formation of a quasicrystalline phase during solidification. The strips were characterised by light microscopy, scanning and transmission electron microscopy, microchemical analysis, and X-ray diffraction. Indentation testing was used to determine the mechanical responses of the strips in different areas. A smooth surface was achieved on both sides of the twin-roll-cast strip, while the free surface of the single-roll-cast strip was rough. The microstructures in both strips consisted of an Al-rich solid solution matrix embedding several intermetallic phases Θ-Al2Cu, Be4Al (Mn, Cu), Al15Mn3Be2 and icosahedral quasicrystalline phase (IQC). The microstructure of the single-roll-cast strip was more uniform than that of the twin-roll-cast strip. Coarse Al15Mn3Be2 particles appeared in both alloys, especially at the centre of the twin-roll strip. These coarse particles adversely affected the strength and ductility. Nevertheless, both casting methods provided high-cooling rates, enabling the formation of metastable phases, such as quasicrystals. However, improvements in alloy composition and casting procedure are required to obtain enhanced microstructures and properties.
Structures and Mechanical Properties of Some Dual-Phase Steels with Low Manganese Content
Authors: Dulucheanu Constantin , Severin Traian Lucian , Cerlinca Delia Aurora , Irimescu Luminita
Published: January 2022

In recent years, to reduce cars costs, research has been conducted on dual-phase steels with low manganese content (below 1.0%). This study investigated the influence of technological parameters of heat treatment (heating temperature and cooling medium) on such steels’ structures and mechanical properties. The ferrite-martensitic structures, specific for dual-phase steels, were obtained by intercritical quenching: heating of samples (made of alloys with 0.511% Mn, respectively 0.529% Mn) to temperatures located between critical points Ac1 and Ac3, followed by cooling in water without mechanical agitation and in water activated with ultrasounds at the frequency of 59 kHz. Through metallographic analyses and tensile tests, it was possible to determine the volume fraction of martensite, the ferrite microhardness, the ultimate tensile strength, the total elongation, and with the obtained data, their variations with the heating temperature and the cooling medium were established. Raising the heating temperature (between 760 °C and 820 °C) and using ultrasounds at cooling increased the volume fraction of martensite and the ferrite microhardness. This fact has increased the mechanical strength and reduced the deformability of the studied dual-phase steels. Intercritical quenching in water activated with ultrasounds provided values of structural characteristics and mechanical properties very close to those obtained by quenching in water without mechanical agitation, but was accomplished using a higher-temperature heating. The results obtained were compared with those determined in previous research, performed on dual-phase steel with 1.90% Mn.
Effects of Al or Mo Addition on Microstructure and Mechanical Properties of Fe-Rich Nonequiatomic FeCrCoMnNi High-Entropy Alloy
Authors: Wang Shuliang , Chen Luyu , Li Qilin , Wang Shidong , Wu Mingyu , Yang Shuiyuan , Xiang Dinghan
Published: January 2022

In this work, a Fe-rich nonequiatomic Fe40Cr15Co15Mn10Ni20 high-entropy alloy was successfully prepared based on phase analysis and cost reduction. Fe40Cr15Co15Mn10Ni20 high-entropy alloy with a single-phase face-centered cubic (FCC) structure was strengthened by the addition of 11 at.% Al or 10 at.% Mo, and the variations of phase and mechanical properties of the strengthened alloys were subsequently investigated. It has been found that the addition of 11 at.% Al led to the formation of FCC and body-centered cubic (BCC) dual-phase structure in the Fe40Cr15Co10Mn4Ni20Al11 alloy, while its yield strength (σ0.2) and tensile strength increased from 158 ± 4 MPa and 420 ± 20 MPa to 218 ± 7 MPa and 507 ± 16 MPa, respectively, as compared to the single-phase FCC structure Fe40Cr15Co15Mn10Ni20 alloy. The addition of 10 at.% Mo introduced intermetallic compounds of μ and σ phases, which resulted in improved yield strength of 246 ± 15 MPa for the Fe40Cr15Co10Mn5Ni20Mo10 alloy. However, the alloy exhibited premature brittle fracture due to the existence of a large number of intermetallic compounds, which led to deteriorated tensile strength of 346 ± 15 MPa. The findings of this work suggest that the introduced secondary phases by the addition of Al and Mo can effectively strengthen the high-entropy alloy; however, the number of intermetallic compounds should be controlled to achieve a combination of high strength and good ductility, which provides a reference for the follow-up study of nonequiatomic high-entropy alloys.
Pharmacokinetic, Metabolomic, and Stability Assessment of Ganoderic Acid H Based Triterpenoid Enriched Fraction of Ganoderma lucidum P. Karst
Authors: Ansari Mohd Hafizur Rehman , Khan Washim , Parveen Rabea , Saher Sadia , Ahmad Sayeed
Published: January 2022

Ganoderma lucidum P. karst is an edible fungus that is used in traditional medicine and contains triterpenoids as the major phytoconstituents. Ganoderic acids are the most abundant triterpenoids that showed pharmacological activity. As Indian varieties contain ganoderic acid H (GA-H), we aimed to prepare GA-H-based triterpenoid enriched fraction (TEF) and evaluated its pharmacokinetics, metabolomics, and stability analysis. A high-performance liquid chromatography (HPLC) method was developed to quantify GA-H in TEF and rat plasma. Based on GA-H content, a stability assessment and pharmacokinetic study of TEF were also performed. After its oral administration to rats, TEF’s the metabolic pattern recognition was performed through ultra-performance liquid chromatography mass spectroscopy (UPLC–MS). The developed HPLC method was found to be simple, sensitive, precise (<15%), and accurate (>90% recovery) for the quantification of GA-H. Pharmacokinetic analysis showed that GA-H reached its maximum plasma concentration (Cmax 2509.9 ng/mL) within two hours and sustained quantifiable amount up to 12 h with a low elimination rate (Kel) 0.05 L/h. TEF contained ten bioavailable constituents. The prepared TEF was found to be stable for up to one year at room temperature. The prepared TEF, enriched with ganoderic acid, is stable, contains bioavailable constituents, and can be explored as phytopharmaceuticals for different pharmacological properties. Highlights: (1). Preparation of triterpenoid enriched fraction (TEF) from Ganoderma lucidum. (2). Major triterpenoid in TEF is ganoderic acid H (GA-H). (3). TEF contains several bioavailable phytoconstituents. (4). TEF (considering only GA-H) is stable for up to one year at room temperature. (5). GA-H is rapidly absorbed and has high systemic exposure.
The LCP Family Protein, Psr, Is Required for Cell Wall Integrity and Virulence in Streptococcus agalactiae
Authors: Rajaei Atefeh , Rowe Hannah M. , Neely Melody N.
Published: January 2022

A robust cell envelope is the first line of protection for an infecting pathogen when encountering the immune defense of its host. In Gram-positive organisms, LytR-CpsA-Psr (LCP) family proteins play a major role in the synthesis and assembly of the cell envelope. While these proteins could be considered for potential new drug targets, not enough is known about how they function to support the integrity of the cell wall. Streptococcus agalactiae (group B streptococcus or GBS) is known to encode at least three LCP family proteins, including CpsA, LytR (BrpA) and Psr. Using strains of GBS that have mutations in two of the three LCP proteins, we were able to determine a role for these proteins in GBS cell wall integrity. The results presented here demonstrate that the absence of Psr results in a decreased growth rate, decreased viability over time, inconsistent cocci morphology and diminished cell wall integrity, as well as an increased penicillin susceptibility, decreased capsule levels and attenuation in virulence in a zebrafish model of infectious disease. A strain that is missing two of the LCP family proteins, CpsA and Psr, exhibits an increase in these defective phenotypes, indicating that CpsA and Psr are partially redundant in function.
Detailed Microparticle Analyses Providing Process Relevant Chemical and Microtextural Insights into the Black Mass
Authors: Dadé Mickaël , Wallmach Thomas , Laugier Odile
Published: January 2022

Eramet uses a combination of physical and hydrometallurgical treatment to recycle lithium-ion batteries. Before hydrometallurgical processing, mechanical treatment is applied to recover the Black Mass which contains nickel, cobalt, manganese and lithium as valuable elements as well as graphite, solvent, plastics, aluminium and copper. To evaluate the suitability for hydrometallurgical recycling, it is essential to analyse the Black Mass chemically but also with respect to size, shape and composition of particles in the Black Mass. The Black Mass of various battery recyclers was investigated by using a combination of SEM/QEMSCAN® analyses. This specific QEMSCAN® database contains 260 subgroups, which comprise major and minor chemical variations of phases. The database was created using millions of point analyses. Major observations are: (1) particles can be micro-texturally characterised and classified with respect to chemical element contents; (2) important textural and chemical particle variations exist in the Black Mass from several origins leading to different levels of quality; (3) elements deleterious to hydrometallurgical processing (i.g. Si, Ca, Ti, Al, Cu and others) are present in well liberated particles; (4) components can be quantified and cathodes active material compositions (LCO, different NMC, NCA, LFP, etc.) that are specific for each battery type can be identified; (5) simulation of further physical mineral processing can optimise Black Mass purity in valuable elements.
Quinoa Soluble Fiber and Quercetin Alter the Composition of the Gut Microbiome and Improve Brush Border Membrane Morphology In Vivo (Gallus gallus)
Authors: Agarwal Nikita , Kolba Nikolai , Khen Noa , Even Carmel , Turjeman Sondra , Koren Omry , Tako Elad
Published: January 2022

Quinoa (Chenopodium quinoa Willd.), a gluten-free pseudo-cereal, has gained popularity over the last decade due to its high nutritional value. Quinoa is a rich source of proteins, carbohydrates, fibers, tocopherols (Vitamin E), unsaturated fatty acids and a wide range of polyphenols. The study used Gallus gallus intra-amniotic feeding, a clinically validated method, to assess the effects of quinoa soluble fiber (QSF) and quercetin 3-glucoside (Q3G) versus control. Quercetin is a pharmacologically active polyphenol found in quinoa. Six groups (no injection, 18 Ω H2O, 5% inulin, 1% Q3G, 5% QSF, 1% Q3G + 5% QSF) were assessed for their effect on the brush border membrane (BBM) functionality, intestinal morphology and cecal bacterial populations. Our results showed a significant (p < 0.05) improvement in BBM morphology, particularly goblet and Paneth cell numbers, in the group administered with quinoa and quercetin. However, there were no significant changes seen in the expression of the genes assessed both in the duodenum and liver between any of the treatment groups. Furthermore, fibrous quinoa increased the concentration of probiotic L. plantarum populations compared to the control (H2O). In conclusion, quercetin and quinoa fiber consumption has the potential to improve intestinal morphology and modulate the microbiome.
Urban Youth Perspectives on Food Insecurity during the COVID-19 Pandemic: Evidence from the COACHES Study
Authors: St. Pierre Christine , Guan Win , Merrill Jamison , Sacheck Jennifer M.
Published: January 2022

School disruptions during the COVID-19 pandemic were a likely threat to food security and exacerbated risk factors associated with poor nutrition and health outcomes among low-income youth. As part of an ongoing school-based study aimed at improving physical activity and dietary behaviors (the COACHES study), associations between youth-reported food insecurity and dietary intake across the pandemic-affected academic year of 2020–2021 were examined. Middle school students (6th and 7th grade, 94% Black/African-American, 92% free-/reduced-price lunch eligible) answered validated surveys on food insecurity and diet and were measured for height and weight for calculation of weight status during Fall 2020 (n = 88) and Spring 2021 (n = 56). During this time, schools underwent a combination of in-person, hybrid, and remote learning. Nearly half of participants were overweight or obese (47%), and self-reported food insecurity was near 30% at both time points. Less than one-third of youth met fruit and vegetable intake guidelines, and more than half drank two or more sugar-sweetened beverages daily. While controlling for sex, maternal education, and weight status, food insecurity was not significantly associated with fruit and vegetable or sugar-sweetened beverage intake. Independent of weight status, youth were aware of being food insecure, yet it did not have an apparent impact on these food groups of concern. These findings highlight the need for greater understanding of youth perceptions of food insecurity in order to adequately address dietary quality and quantity concerns among children.
Chair-Stand Exercise Improves Sarcopenia in Rehabilitation Patients after Stroke
Authors: Yoshimura Yoshihiro , Wakabayashi Hidetaka , Nagano Fumihiko , Bise Takahiro , Shimazu Sayuri , Shiraishi Ai , Kido Yoshifumi , Matsumoto Ayaka
Published: January 2022

Currently, there is a lack of evidence to show that exercise therapy improves sarcopenia in older patients in clinical practice. We therefore conducted a retrospective cohort study to clarify the effects of chair-stand exercise on improving sarcopenia among patients diagnosed with sarcopenia undergoing convalescent rehabilitation after stroke. According to the latest Asian criteria, sarcopenia was diagnosed when both skeletal muscle mass index (SMI) and handgrip strength (HGS) were low. Patients were asked to perform a repeated chair-stand exercise as whole-body resistance training, in addition to the rehabilitation program. Outcomes included sarcopenia rates, SMI, HGS, and physical function at hospital discharge. Multivariate analyses were used to examine whether the frequency of daily chair-stand exercise was independently associated with the outcomes after adjustment for potential confounders. After enrollment, 302 patients with sarcopenia (mean age: 78.6 years; 46.4% male) were analyzed. Overall, sarcopenia prevalence decreased by 21.9%, from 100% at admission to 78.1% at discharge. Multivariate analyses showed that the frequency of the exercise was significantly associated with the presence of sarcopenia (odds ratio: 0.986, p = 0.010), SMI (β = 0.181, p < 0.001), and HGS (β = 0.101, p = 0.032) at discharge, respectively. The chair-standing exercise was effective in improving sarcopenia in these patients.
Skin in the Game: An Assay to Monitor Leukocyte Infiltration in Dermal Lesions of a Guinea Pig Model for Tick-Borne Rickettsiosis
Authors: Cross Claire E. , Stokes John V. , Alugubelly Navatha , Ross Anne-Marie L. , Willeford Bridget V. , Walker Jamie D. , Varela-Stokes Andrea S.
Published: January 2022

Intact, the skin typically serves as an effective barrier to the external world; however, once pathogens have breached this barrier via a wound, such as a tick bite, the surrounding tissues must recruit immune cells from the blood to neutralize the pathogen. With innate and adaptive immune systems being similar between the guinea pig and human systems, the ability of guinea pigs to show clinical signs of many infectious diseases, and the large size of guinea pigs relative to a murine model, the guinea pig is a valuable model for studying tick-borne and other pathogens that invade the skin. Here, we report a novel assay for assessing guinea pig leukocyte infiltration in the skin. Briefly, we developed an optimized six-color/eight-parameter polychromatic flow cytometric panel that combines enzymatic and mechanical dissociation of skin tissue with fluorescent antibody staining to allow for the immunophenotyping of guinea pig leukocytes that have migrated into the skin, resulting in inflammation. We designed this assay using a guinea pig model for tick-borne rickettsiosis to further investigate host–pathogen interactions in the skin, with preliminary data demonstrating immunophenotyping at skin lesions from infected ticks. We anticipate that future applications will include hypothesis testing to define the primary immune cell infiltrates responding to exposure to virulent, avirulent tick-borne rickettsiae, and tick-borne rickettsiae of unknown virulence. Other relevant applications include skin lesions resulting from other vector-borne pathogens, Staphylococcus aureus infection, and Buruli ulcer caused by Mycobacterium ulcerans.
Genomic Investigation of Two Acinetobacter baumannii Outbreaks in a Veterinary Intensive Care Unit in The Netherlands
Authors: Naing Soe Yu , Hordijk Joost , Duim Birgitta , Broens Els M. , van der Graaf-van Bloois Linda , Rossen John W. , Robben Joris H. , Leendertse Masja , Wagenaar Jaap A. , Zomer Aldert L.
Published: January 2022

Acinetobacter baumannii is a nosocomial pathogen that frequently causes healthcare-acquired infections. The global spread of multidrug-resistant (MDR) strains with its ability to survive in the environment for extended periods imposes a pressing public health threat. Two MDR A. baumannii outbreaks occurred in 2012 and 2014 in a companion animal intensive care unit (caICU) in the Netherlands. Whole-genome sequencing (WGS) was performed on dog clinical isolates (n = 6), environmental isolates (n = 5), and human reference strains (n = 3) to investigate if the isolates of the two outbreaks were related. All clinical isolates shared identical resistance phenotypes displaying multidrug resistance. Multi-locus Sequence Typing (MLST) revealed that all clinical isolates belonged to sequence type ST2. The core genome MLST (cgMLST) results confirmed that the isolates of the two outbreaks were not related. Comparative genome analysis showed that the outbreak isolates contained different gene contents, including mobile genetic elements associated with antimicrobial resistance genes (ARGs). The time-measured phylogenetic reconstruction revealed that the outbreak isolates diverged approximately 30 years before 2014. Our study shows the importance of WGS analyses combined with molecular clock investigations to reduce transmission of MDR A. baumannii infections in companion animal clinics.
Local Emergence of a del HV69-70 SARS-CoV-2 Variant in Burgundy, France
Authors: Giraudon Hélène , Djemai Mohand , Auvray Christelle , de Rougemont Alexis , Belliot Gaël , Bour Jean-Baptiste , Manoha Catherine
Published: January 2022

In the autumn of 2020, a short-lived epidemic of a spike del69-70 deletion variant of SARS-CoV-2 was identified, with most cases (n = 95) found in Montceau-les-Mines, France. This spike gene target failure (SGTF) variant spread quickly in nursing homes. The Alpha variant, which also harbors this deletion, appeared in Burgundy in January 2021 after the disappearance of the Montceau-les-Mines del69-70 variant. Our findings illustrate the risk of the fast spread of geographically isolated variants and reinforce the need for the continuous tracking of outbreaks. In some cases, these studies may reveal emerging variants that affect public health or vaccine development.