bluuebunny/crossref_metadata_2025 huggingface dataset:
Identification of a ferroptosis-related gene signature predicting recurrence in stage II/III colorectal cancer based on machine learning algorithms
Authors: Wang Ze , Ma Chenghao , Teng Qiong , Man Jinyu , Zhang Xuening , Liu Xinjie , Zhang Tongchao , Chong Wei , Chen Hao , Lu Ming
Published: August 2023

Background: Colorectal cancer (CRC) is one of the most prevalent cancer types globally. A survival paradox exists due to the inherent heterogeneity in stage II/III CRC tumor biology. Ferroptosis is closely related to the progression of tumors, and ferroptosis-related genes can be used as a novel biomarker in predicting cancer prognosis.

Methods: Ferroptosis-related genes were retrieved from the FerrDb and KEGG databases. A total of 1,397 samples were enrolled in our study from nine independent datasets, four of which were integrated as the training dataset to train and construct the model, and validated in the remaining datasets. We developed a machine learning framework with 83 combinations of 10 algorithms based on 10-fold cross-validation (CV) or bootstrap resampling algorithm to identify the most robust and stable model. C-indice and ROC analysis were performed to gauge its predictive accuracy and discrimination capabilities. Survival analysis was conducted followed by univariate and multivariate Cox regression analyses to evaluate the performance of identified signature.

Results: The ferroptosis-related gene (FRG) signature was identified by the combination of Lasso and plsRcox and composed of 23 genes. The FRG signature presented better performance than common clinicopathological features (e.g., age and stage), molecular characteristics (e.g., BRAF mutation and microsatellite instability) and several published signatures in predicting the prognosis of the CRC. The signature was further stratified into a high-risk group and low-risk subgroup, where a high FRG signature indicated poor prognosis among all collected datasets. Sensitivity analysis showed the FRG signature remained a significant prognostic factor. Finally, we have developed a nomogram and a decision tree to enhance prognosis evaluation.

Conclusion: The FRG signature enabled the accurate selection of high-risk stage II/III CRC population and helped optimize precision treatment to improve their clinical outcomes.
A 3-year application of different mycorrhiza-based plant biostimulants distinctively modulates photosynthetic performance, leaf metabolism, and fruit quality in grapes (Vitis vinifera L.)
Authors: Ganugi Paola , Caffi Tito , Gabrielli Mario , Secomandi Elena , Fiorini Andrea , Zhang Leilei , Bellotti Gabriele , Puglisi Edoardo , Fittipaldi Monica Broussard , Asinari Florencia , Tabaglio Vincenzo , Trevisan Marco , Lucini Luigi
Published: August 2023

The use of microbial biostimulants in agriculture is recognized as a sustainable approach to promoting crop productivity and quality due to improved nutrient uptake, enhanced stress tolerance, and improved ability to cope with non-optimal environments. The present paper aimed to comparatively investigate the effect of seven different commercial mycorrhizal-based treatments in terms of yield, phytochemical components, and technological traits of Malvasia di Candia Aromatica grape (Vitis vinifera L.) plants. Metabolomic analysis and photosynthetic performance were first investigated in leaves to point out biochemical differences related to plant growth. Higher photosynthetic efficiency and better PSII functioning were found in biostimulant-treated vines, reflecting an overall decrease in photoinhibition compared to untreated plants. Untargeted metabolomics followed by multivariate statistics highlighted a robust reprogramming of primary (lipids) and secondary (alkaloids and terpenoids) metabolites in treated plants. The analysis of berry yield and chemical components exhibited significant differences depending on the biostimulant product. Generally, berries obtained from treated plants displayed improved contents of polyphenols and sugars, while yield remained unchanged. These results elucidated the significant role of microbial biostimulants in determining the quality of grape berries and eliciting biochemical changes in vines.
Perceived offensiveness to the self, not that to others, is a robust positive predictor of support of censoring sexual, alcoholic, and violent media content
Authors: Zhang Jinguang
Published: August 2023

Introduction

Harm and offense are two important notions in legal discussions on the extent to which one’s freedom may be limited. Prior research on the third-person effect found that perceived media harm on others, not perceived media harm on the self, is a robust positive predictor of support of censoring socially undesirable media content (e.g., pornography). In comparison, how offensiveness perceptions predict censorship support is not clear. Drawing on moral foundations theory, we test here how perceived media offensiveness to the self compared with 1) perceived media offensiveness to others and 2) perceived media harm on others would predict censorship support.

Method

We conducted two cross-sectional survey studies in the U.S. to address this question with sexual, alcoholic, and violent media content as test cases. In Study 1 (N = 544 undergraduates), we measured perceived media offensiveness to the self, that to others, and censorship support. In Study 2 (N = 727 non-student adults), we also measured perceived media harm on the self and others.

Results

As in prior research, we found that people perceive sexual, alcoholic, and violent media content to harm other viewers more strongly than it harms themselves, and the perception of how much others are harmed predicts perceivers’ censorship support. In contrast, while people also perceive the three types of media content to offend other viewers more strongly than they offend the self, the perception of how much others are offended predicts censorship support to a significantly lesser extent or does not predict this at all. Instead, the perception of how much the self is offended does.

Discussion

These findings add to the work on moral foundations theory that distinguishes between how the care/harm and sanctity/degradation foundations relate to moral judgments. These findings also suggest that the current theorizing of the third-person effect needs to expand to reconcile the seemingly inconsistent results on how harm and offensiveness perceptions differently relate to censorship support. The care/harm and sanctity/degradation foundations may underlie how harm and offensiveness perceptions predict censorship support. However, several “anomalous” findings need to be accounted for before moral foundations provide a comprehensive explanation of the third-person effect.
Spare the rod, spoil the child: measurement and learning from an intervention to shift corporal punishment attitudes and behaviors in Grenada, West Indies
Authors: Landon Barbara , Thomas Elizabeth D. , Orlando Lauren , Evans Roberta , Murray Toni , Mohammed Lauren , Noel Jesma , Isaac Rashida , Waechter Randall
Published: August 2023

Childrearing practices in the Caribbean and other postcolonial states have long been associated with corporal punishment and are influenced by expectations of children for respectfulness and obedience. Evidence across settings shows that physical punishment of young children is both ineffective and detrimental. Saving Brains Grenada (SBG) implemented a pilot study of an intervention based on the Conscious Discipline curriculum that aimed to build adult caregivers’ skills around non-violent child discipline. We hypothesized that attitudes towards corporal punishment would shift to be negative as adults learned more positive discipline methods, and that child neurodevelopment would correspondingly improve. This report reviews the impact of monitoring and evaluation on the design and implementation of the intervention. Study 1 presents findings from the pilot study. Despite positive gains in neurodevelopmental outcomes among children in the intervention compared to controls, attitudes towards corporal punishment and reported use of it did not change. Additionally, several internal conflicts in the measures used to assess corporal punishment behaviors and attitudes were identified. Study 2 is a response to learning from Study 1 and highlights the importance for monitoring and evaluation to be data-informed, adaptive, and culturally appropriate. In Study 2, the SBG research team conducted cognitive interviews and group discussions with stakeholders to assess the content and comprehensibility of the Attitudes Towards Corporal Punishment Scale (ACP). This yielded insights into the measurement of attitudes towards corporal punishment and related parenting behavior, and prompted several revisions to the ACP. To accurately evaluate the intervention’s theory of change and its goal to reduce violence against children, reliable and appropriate measures of attitudes towards corporal punishment and punishment behaviors are needed. Together, these two studies emphasize the value of continuous monitoring, evaluation, and learning in the implementation, adaptation, evaluation, and scaling of SBG and similar early childhood development interventions.
European Society for Organ Transplantation Consensus Statement on Biomarkers in Liver Transplantation
Authors: Berenguer Marina , de Martin Eleonora , Hessheimer Amelia J. , Levitsky Josh , Maluf Daniel G. , Mas Valeria R. , Selzner Nazia , Hernàndez-Èvole Helena , Lutu Alina , Wahid Nabeel , Zubair Haseeb
Published: August 2023

Currently, one-year survival following liver transplantation (LT) exceeds 90% in large international registries, and LT is considered definitive treatment for patients with end-stage liver disease and liver cancer. Recurrence of disease, including hepatocellular carcinoma (HCC), significantly hampers post-LT outcomes. An optimal approach to immunosuppression (IS), including safe weaning, may benefit patients by mitigating the effect on recurrent diseases, as well as reducing adverse events associated with over-/under-IS, including chronic kidney disease (CKD). Prediction of these outcome measures—disease recurrence, CKD, and immune status—has long been based on relatively inaccurate clinical models. To address the utility of new biomarkers in predicting these outcomes in the post-LT setting, the European Society of Organ Transplantation (ESOT) and International Liver Transplant Society (ILTS) convened a working group of experts to review literature pertaining to primary disease recurrence, development of CKD, and safe weaning of IS. Summaries of evidence were presented to the group of panelists and juries to develop guidelines, which were discussed and voted in-person at the Consensus Conference in Prague November 2022. The consensus findings and recommendations of the Liver Working Group on new biomarkers in LT, clinical applicability, and future needs are presented in this article.
Portuguese Agrifood Sector Resilience: An Analysis Using Structural Breaks Applied to International Trade
Authors: Oliveira Maria de Fátima , Reis Pedro
Published: August 2023

In the last two decades, Portugal suffered the effects of two global crises, the financial crisis and the COVID-19 pandemic, as well as the Common Agriculture Policy reforms. These crises had a great impact on the Portuguese economy, but it is completely unclear how they affected the dynamics of the Portuguese agrifood sector. This study’s objective is to analyze the resilience of this sector to European and global socks, testing the effects on international trade. Secondary data from the Portuguese Statistics Institute were used for the exports and imports trade series of animal and vegetable products and food industries from 2000 to 2020. The methodology was based on the structural xtbreak model, stability analysis, and tests for structural breaks. Some volatility was observed in the trade series, particularly in imports, without consistency among years, trade sectors, or imports versus exports trade. In the case of exports, one or two structural breaks in the different sectors occurred in different years. The most relevant dynamics occurred after the sovereign debt crisis. It was concluded that CAP reforms and global crises seem to not have caused new relevant dynamics in the Portuguese international agrifood trade. This revealed the resilience of the sector to external shocks.
An Environmental and Economic Analysis of Strawberry Production in Southern Italy
Authors: Pergola Maria , Maffia Angela , Carlucci Giuseppe , Persiani Alessandro , Palese Assunta Maria , Zaccardelli Massimo , Altieri Gessica , Celano Giuseppe
Published: August 2023

This paper aims to provide an evaluation of the environmental and economic aspects of strawberry cultivation in the Campania and Basilicata regions of Southern Italy, and to consider the effects on strawberry productivity following compost tea (CT) application. Eight strawberry-growing systems were tested. To this end, compost tea production and characterization were described; a quantitative analysis of the strawberries’ yield was performed, and environmental impact per ha and per kg of strawberries was estimated using the life cycle assessment methodology. To compare the profitability of the systems analyzed, the gross profit of the farmers was calculated, also considering the social cost of pollution. One of the two organic systems analyzed, using solarization for soil disinfestation, biological fight for pest control, and corrugated boxes as packaging recycled at the end-of-life, was the most sustainable system with carbon credits. At the same time, organic crops are not always the most sustainable and profitable systems if significant irrigation and fertigation interventions are carried out, as in another organic system analyzed. Plastic materials and zinc structures were the most impacting items in almost all analyzed systems. The use of a CT with an elevated number of beneficial microorganisms with a high suppressive action allowed to obtain a good increase of the yield, in both systems that used it, and to have a higher gross profit. On the other hand, the validity of this technique was strongly linked to the finding of high-quality green compost.
Elevated CO2 Influences the Growth, Root Morphology, and Leaf Photosynthesis of Cacao (Theobroma cacao L.) Seedlings
Authors: Ishida Akiko , Ogiwara Isao , Suzuki Sakae
Published: August 2023

Growing quality seedlings is a challenge for sustainable cacao production as the survival rate of young seedlings is strongly influenced by environmental factors that affect the productivity of cacao farmers. In this study, cacao (Theobroma cacao L.) seedlings were cultivated in a nursery, and the effects of elevated CO2 concentrations (approximately 800 ppm) applied to cacao seedlings during daytime (6:00–17:59) on the root growth, morphology, and leaf photosynthetic capacity were examined. Treatment with elevated CO2 significantly improved root growth, dry matter weight, and root/shoot ratio. Three-dimensional imaging of roots showed that lateral roots grew longer horizontally, lateral roots and fine roots were distributed over a larger area, and root surface and root volume increased significantly under elevated CO2 treatment. Accurate quantification of root morphology using X-ray CT indicated that the treatment with elevated CO2 concentrations may significantly affect root quality during the seedling stage by expanding the distribution range of lateral and fine roots, which increases the ability of lateral roots to elongate and absorb water and nutrients from the superficial layers. The photosynthetic characteristics of the aboveground leaves of cacao seedlings exposed to elevated CO2 concentrations showed a tendency to adapt to elevated CO2 concentrations by increasing light-use efficiency and CO2-use efficiency. Therefore, the treatment of cacao seedlings with elevated CO2 concentrations improved the growth quality of seedlings due to the characteristics of the roots as large sinks.
Effect of Rootstock Selection on Tree Growth, Yield, and Fruit Quality of Lemon Varieties Cultivated in Greece
Authors: Ziogas Vasileios , Kokkinos Epameinondas , Karagianni Antonia , Ntamposi Evgenia , Voulgarakis Apostolis Stilianos , Hussain Syed Bilal
Published: August 2023

Lemon is a prominent citrus fruit that supports regional economies worldwide. Several agronomic and fruit quality attributes are affected by the rootstock selection, thus its selection is essential for a successful grove. This study aims to compare the agronomic and quality attributes of four lemon cultivars (‘Mikrokarpo Messaras’, ‘Nouvel Athos’, ‘Femminello Commune,’ and ‘Zia gara Bianca’) grafted onto three rootstocks (‘Sour Orange’, ‘Yuma Ponderosa lemon’, and ‘Volkameriana’). The yield and rootstock/scion ratio were estimated along with fruit morphological characteristics (size, equatorial, and polar diameter). Internal fruit quality was also estimated (total soluble solids, total acidity, juiciness, ascorbic acid, total phenols, and total antioxidant activity). ‘Volkameriana’ rootstock stood out among the studied rootstocks, as all grafted lemon varieties increased their productivity. Its combination with the Italian cultivar ‘Femminello Commune’ exhibited enhanced tree vigor and tree yield. For all studied cultivars, the use of ‘Volkameriana’ or ‘Yuma Ponderosa lemon’ rootstocks decreased the total soluble content and total acidity, increased the ascorbic acid content, and did not influence the TSS/TA ratio, total phenols or total antioxidant activity. In conclusion, the vigorous ‘Volkameriana’ and ‘Yuma Ponderosa lemon’ rootstocks are a promising replacement for ‘sour orange’. This research provides valuable insights into the potential use of ‘Yuma Ponderosa lemon’ as a rootstock for lemons, as existing literature is rather limited.
Interspecific Competition as Affected by Nitrogen Application in Sweet Corn–Soybean Intercropping System
Authors: Yu Xia , Xiao Shihao , Yan Tingxian , Chen Zhongping , Zhou Quan , Pan Yuzhuo , Yang Wenting , Lu Meijuan
Published: August 2023

Corn (Zea mays L.)–soybean (Glycine max (L.) Merr.) intercropping is one of the main traditional intercropping systems used. We hypothesized that sweet corn–soybean intercropping with reduced nitrogen application could improve the crops’ fresh grain yield and nitrogen acquisition. We clarified whether sweet corn intercropped with soybean has the advantages of improved crop yield and carbon and nitrogen accumulation and assessed interspecific competition in the intercropping systems. A four-year (2017–2020) field experiment was conducted with three nitrogen application levels (0, 150, and 300 kg∙ha−1) and three planting patterns (monocropped sweet corn, monocropped soybean, sweet corn–soybean intercropping) at Jiangxi Agricultural University, Nanchang, China. The LER (land equivalent ratio), AG (aggressivity), and CR (competitive ratio) were calculated using the fresh grain yield and nitrogen and carbon accumulation of sweet corn and soybean. The LER values were greater than 1.0 in most of the intercropped patterns, except for the value based on the crops’ fresh grain yield without nitrogen application in 2020. Sweet corn had greater values of CR and AG than soybean in the intercropping system. Compared with common nitrogen application (300 kg∙ha−1), reduced nitrogen application (150 kg∙ha−1) did not significantly reduce the LER or the average CR and AG values. Under reduced nitrogen application, the values of LER, CR, and AG, based on the crops’ fresh grain yield and nitrogen acquisition, were not significantly different between the four years. In conclusion, based on the LER, CR, and AG, sweet corn–soybean intercropping had the advantage of crop yield and nitrogen acquisition, and sweet corn was the superior competitor. Sweet corn–soybean intercropping with nitrogen application (150 kg N ha−1) showed good inter-annual stability of crop productivity and competitiveness of the sweet corn.
Leaf Area Duration and Crop Radiation Use Efficiency Determine Biomass Yield of Lignocellulosic Perennial Grasses under Different Soil Water Content
Authors: Corinzia Sebastiano Andrea , Crapio Elena , Testa Giorgio , Cosentino Salvatore L. , Patanè Cristina , Scordia Danilo
Published: August 2023

The aim of the present work was to assess the leaf area duration (LAD) and the radiation use efficiency (RUE) of six warm-season perennial biomass grasses (PBGs) in a two-year field trial in the semiarid Mediterranean climate under different soil water availability. Two ecotypes of giant reed (Arundo donax L., ARCT and ARMO), one ecotype of African fodder cane (Saccharum spontaneum L. subsp. aegyptiacum (Willd.) Hack., SAC) and three hybrids of Miscanthus (the commercial M. × giganteus J.M. Greef, Deuter ex Hodk., Renvoize, M × G, and two new seed-based hybrids, GNT9 and GNT10) were compared under three levels of soil water availability: rainfed, 50% and 100% of maximum crop evapotranspiration (ETm) restoration. The determination of RUE of perennial plants is controversial and has led to contrasting results in past studies. In the present work, LAD and RUE differed among crops and irrigation regimes, being positively affected by supplemental water inputs. SAC, ARCT and ARMO showed both high LAD and RUE, which determined the high biomass yield than both the commercial M × G and the improved Miscanthus hybrids GNT9 and GNT10. RUE was particularly high and less affected by soil water availability during the mid-season, while the effect of irrigation and the differences among the genotypes were larger during the late season. Adequate biomass yield can be achieved by sub-optimal soil water availability, thus reducing the water footprint and increasing the sustainability of these biomass perennial grasses selected for the Mediterranean climate.
Exogenous Selenium Endows Salt-Tolerant and Salt-Sensitive Soybeans with Salt Tolerance through Plant-Microbial Coactions
Authors: Wang Yin , Xu Chao , Wuriyanghan Hada , Lei Zheng , Tang Yanni , Zhang Huan , Zhao Xiaohu
Published: August 2023

Soil salinization is a common abiotic stress that seriously affects soybean growth and yield, underscoring the need to enhance plant salt tolerance for sustainable agriculture development. Selenium is a beneficial element that has been shown to promote plant growth, development and stress resistance. This study employed pot experiments to investigate the effects of different salt levels (0, 50, 100 and 150 mM NaCl) on salt-tolerant (Zhonghuang 13) and salt-sensitive soybean (Dongnong 63) varieties. Additionally, the critical salt concentration (100 mM NaCl) was selected to explore the effects of exogenous selenium (0, 0.5, 1 and 3 mg·kg−1) on improving salt tolerance in salt-tolerant and salt-sensitive soybeans under salt stress. Results showed that as salt concentration increased, plant height, shoot and root fresh weight, SPAD value and enzyme activity of both salt-tolerant and salt-sensitive soybeans significantly decreased. The increasing concentration of exogenous selenium significantly decreased the proline content of salt-sensitive and salt-tolerant soybeans by 40.65–58.87% and 38.51–50.46%, respectively, and the MDA content by 19.33–30.36% and 16.94–37.48%, respectively. Selenium supplementation also reduced the content of Na+ in salt-sensitive and salt-tolerant soybeans and improved K+ absorption in soybeans, which increased the K+/Na+ ratio. Moreover, high-throughput sequencing of the 16S ribosomal RNA gene demonstrated that selenium application optimized the rhizosphere microecology structure of salt-tolerant and salt-sensitive soybean varieties and enhanced functional genes related to lipid metabolism, energy metabolism and cell motility of rhizosphere microorganisms. In summary, selenium application improved the salt tolerance of the two soybean varieties by enhancing the physiological resistance to salt stress and optimizing the structure and function of the rhizosphere microbial community.
Total Soluble Solids in Grape Must Estimation Using VIS-NIR-SWIR Reflectance Measured in Fresh Berries
Authors: Mejía-Correal Karen Brigitte , Marcelo Víctor , Sanz-Ablanedo Enoc , Rodríguez-Pérez José Ramón
Published: August 2023

Total soluble solids (TSS) is a key variable taken into account in determining optimal grape maturity for harvest. In this work, partial least square (PLS) regression models were developed to estimate TSS content for Godello, Verdejo (white), Mencía, and Tempranillo (red) grape varieties based on diffuse spectroscopy measurements. To identify the most suitable spectral range for TSS prediction, the regression models were calibrated for four datasets that included the following spectral ranges: 400–700 nm (visible), 701–1000 nm (near infrared), 1001–2500 nm (short wave infrared) and 400–2500 nm (the entire spectral range). We also tested the standard normal variate transformation technique. Leave-one-out cross-validation was implemented to evaluate the regression models, using the root mean square error (RMSE), coefficient of determination (R2), ratio of performance to deviation (RPD), and the number of factors (F) as evaluation metrics. The regression models for the red varieties were generally more accurate than the models of those for the white varieties. The best regression model was obtained for Mencía (red): R2 = 0.72, RMSE = 0.55 °Brix, RPD = 1.87, and factors n = 7. For white grapes, the best result was achieved for Godello: R2 = 0.75, RMSE = 0.98 °Brix, RPD = 1.97, and factors n = 7. The methodology used and the results obtained show that it is possible to estimate TSS content in grapes using diffuse spectroscopy and regression models that use reflectance values as predictor variables. Spectroscopy is a non-invasive and efficient technique for determining optimal grape maturity for harvest.
YOLOv5-AC: A Method of Uncrewed Rice Transplanter Working Quality Detection
Authors: Wang Yue , Fu Qiang , Ma Zheng , Tian Xin , Ji Zeguang , Yuan Wangshu , Kong Qingming , Gao Rui , Su Zhongbin
Published: August 2023

With the development and progress of uncrewed farming technology, uncrewed rice transplanters have gradually become an indispensable part of modern agricultural production; however, in the actual production, the working quality of uncrewed rice transplanters have not been effectively detected. In order to solve this problem, a detection method of uncrewed transplanter omission is proposed in this paper. In this study, the RGB images collected in the field were inputted into a convolutional neural network, and the bounding box center of the network output was used as the approximate coordinates of the rice seedlings, and the horizontal and vertical crop rows were fitted by the least square method, so as to detect the phenomenon of rice omission. By adding atrous spatial pyramid pooling and a convolutional block attention module to YOLOv5, the problem of image distortion caused by scaling and cropping is effectively solved, and the recognition accuracy is improved. The accuracy of this method is 95.8%, which is 5.6% higher than that of other methods, and the F1-score is 93.39%, which is 4.66% higher than that of the original YOLOv5. Moreover, the network structure is simple and easy to train, with the average training time being 0.284 h, which can meet the requirements of detection accuracy and speed in actual production. This study provides an effective theoretical basis for the construction of an uncrewed agricultural machinery system.
Genome-Wide Association Study to Identify QTL for Carcass Traits in Korean Hanwoo Cattle
Authors: Alam Mohammad Zahangir , Haque Md Azizul , Iqbal Asif , Lee Yun-Mi , Ha Jae-Jung , Jin Shil , Park Byoungho , Kim Nam-Young , Won Jeong Il , Kim Jong-Joo
Published: August 2023

This study aimed to identify genetic associations with carcass traits in Hanwoo cattle using a genome-wide association study. A total of 9302 phenotypes were analyzed, and all animals were genotyped using the Illumina Bovine 50K v.3 SNP chip. Heritabilities for carcass weight (CWT), eye muscle area (EMA), backfat thickness (BF), and marbling score (MS) were estimated as 0.42, 0.36, 0.36, and 0.47, respectively, using the GBLUP model, and 0.47, 0.37, 0.36, and 0.42, respectively, using the Bayes B model. We identified 129 common SNPs using DGEBV and 118 common SNPs using GEBV on BTA6, BTA13, and BTA14, suggesting their potential association with the traits of interest. No common SNPs were found between the GBLUP and Bayes B methods when using residuals as a response variable in GWAS. The most promising candidate genes for CWT included SLIT2, PACRGL, KCNIP4, RP1, XKR4, LYN, RPS20, MOS, FAM110B, UBXN2B, CYP7A1, SDCBP, NSMAF, TOX, CA8, LAP3, FAM184B, and NCAPG. For EMA, the genes IBSP, LAP3, FAM184B, LCORL, NCAPG, SLC30A9, and BEND4 demonstrated significance. Similarly, CYP7B1, ARMC1, PDE7A, and CRH were associated with BF, while CTSZ, GNAS, VAPB, and RAB22A were associated with MS. This finding offers valuable insights into genomic regions and molecular mechanisms influencing Hanwoo carcass traits, aiding efficient breeding strategies.
Lactobacillus plantarum Decreased Ammonia Emissions through Modulating Cecal Microbiotain Broilers Challenged with Ammonia
Authors: Liu Xiyue , Cao Guangtian , Qiu Kaifan , Dong Yingkun , Hu Caihong
Published: August 2023

Probiotic supplementation has become a prominent method of decreasing ammonia emissions in poultry production. The present study was conducted to investigate the influence of Lactobacillus plantarum on ammonia emission, immune responses, antioxidant capacity, cecal microflora and short chain fatty acids, and serum metabolites in broilers challenged with ammonia. A total of 360 1-day-old yellow-feathered broilers were randomly divided into three treatment groups: birds fed with a basal diet (CON), a basal diet supplemented with ammonia (AN), and a basal diet supplemented with 2.5 × 108 CFU L. plantarum kg−1 and challenged with ammonia (LP). Data showed that L. plantarum supplementation decreased ammonia more than 30% from day 48, and significantly reduced the levels of serum urea nitrogen and ammonia, fecal urease, and ammonium nitrogen compared with those on CON. Compared with AN and CON treatments, LP administration increased (p < 0.05) the concentration of serum immunoglobulin Y (IgY), IgM, and IL-10, as well as the serum total-antioxidant capacity (T-AOC) and GSH-Px, and decreased (p < 0.05) IL-1β, IL-6, and TNF-α. Furthermore, birds supplemented with LP had higher (p < 0.05) cecal contents of short chain fatty acids (SCFAs) than AN birds and had more butyrate than CON birds. Data from 16s high throughput sequencing showed that LP supplementation significantly increased (p < 0.05) the Shannon and Simpson indices of bird cecal microflora, and Alloprevotella dominated the LP birds. The function prediction of cecal microflora indicated that LP treatment significantly increased alanine aspartate and glutamate metabolism, starch and sucrose metabolism, exosome, mismatch repair, homologous recombination, DNA repair and recombination proteins, and amino acid-related enzymes. The serum metabolome showed that LP supplementation significantly changed the aminoacyl-tRNA, pantothenate and acetyl-coenzyme A, arginine, phenylalanine, tyrosine and tryptophan, valine, leucine, and isoleucine biosynthesis; purine, beta-alanine, galactose, histidine, alanine, aspartate and glutamate, glyoxylate and dicarboxylate, pyruvate and thiamine metabolism, melanogenesis, and citrate cycle.
Reduced Nitric Oxide Synthase Involvement in Aigamo Duck Basilar Arterial Relaxation
Authors: Wu Siyuan , Ootawa Tomoki , Sekio Ryoya , Smith Henry , Islam Md. Zahorul , Nguyen Ha Thi Thanh , Uno Yasuhiro , Shiraishi Mitsuya , Miyamoto Atsushi
Published: August 2023

The basilar arterial endothelium mediates blood vessel relaxation partly through the release of nitric oxide (NO). Apoptosis of cerebrovascular endothelial cells is linked to a high mortality rate in chickens infected with the highly pathogenic avian influenza virus, but interestingly, ducks exhibit a greater resistance to this virus. In this study, we examined the responsiveness of duck basilar arteries (BAs) to various vasoactive substances, including 5-hydroxytryptamine (5-HT), histamine (His), angiotensin (Ang) II, noradrenaline (NA), acetylcholine (ACh), and avian bradykinin ornithokinin (OK), aiming to characterize the receptor subtypes involved and the role of endothelial NO in vitro. Our findings suggest that arterial contraction is mediated with 5-HT1 and H1 receptors, while relaxation is induced with β3-adrenergic and M3 receptors. Additionally, OK elicited a biphasic response in duck BAs, and Ang II had no effect. Endothelial NO appears to be crucial in relaxation mediated with M3 and OK receptors but not β3-adrenergic receptors in the duck BA. The reduced endothelial NO involvement in the receptor-mediated relaxation response in duck BAs represents a clear difference from the corresponding response reported in chicken BAs. This physiological difference may explain the differences in lethality between ducks and chickens when vascular endothelial cells are infected with the virus.
Selective Anthelmintic Treatment in Horses in Sweden Based on Coprological Analyses: Ten-Year Results
Authors: Osterman-Lind Eva , Holmberg Mia , Grandi Giulio
Published: August 2023

In Sweden, routine deworming has been used for several decades; however, to slow down the development of anthelmintic resistance, selective treatment is currently recommended. As part of a monitoring programme, equestrian premises submitted faecal samples to the National Veterinary Institute (SVA) twice per year between 2008 and 2017. Analyses for strongyles (small and large), tapeworms and ascarids, followed by premise-specific advice regarding deworming and parasite control strategies, were provided. In total, 43,330 faecal samples, collected from 26,625 horses on 935 premises in springtime (March to June), were analysed by quantitative or semi-quantitative flotation. Moreover, Strongylus vulgaris was detected by larval culture or PCR. Between 4 and 11% of individual horses tested positive for S. vulgaris and 3–10% were shedding tapeworm eggs. There were recurrent high and low egg shedders; 75% of horses with S. vulgaris appeared to have been recently introduced into the herd; the proportion of S. vulgaris-positive premises increased when individual samples rather than pooled samples were used. Based on the results of S. vulgaris diagnostics and strongyle egg-shedding level, 59% of the horses did not need to be dewormed.
Dietary Inclusion of Halobacterium salinarum Modulates Growth Performances and Immune Responses in Farmed Gilthead Seabream (Sparus aurata L.)
Authors: Messina Concetta Maria , Madia Manfredi , Manuguerra Simona , Espinosa-Ruiz Cristobal , Esteban María Angeles , Santulli Andrea
Published: August 2023

The use of natural immunostimulants is considered the most promising alternative to promote fish health, productive performance and quality, increasing the aquaculture profitability, sustainability and social acceptance. The purpose of this study was to evaluate the effect of the integration of a potential probiotic strain, Halobacterium salinarum, belonging to the Archaea domain, in the formulated diets of farmed gilthead seabream (Sparus aurata L.) in terms of growth performances and immunity responses. The experiment was set up to test two different levels of inclusion of the bacteria in the diet: 0.05% (D1) and 0.1% (D2). The effects on fish growth performances; humoral (peroxidase, protease, antiprotease and IgM levels) and cellular immunity parameters (phagocytosis, respiratory burst and myeloperoxidase), along with bactericidal activity, were evaluated after 15 and 30 days of experimental feeding. The obtained results showed that the inclusion of H. salinarum at the highest concentration (D2 0.1%) improved growth performances, bactericidal activity against Vibrio anguillarum and some parameters related both to the humoral and cellular immune response, suggesting exploring other aspects of welfare in view of future supplementations of this probiotic strain in the diet of S. aurata.
Cerebral Vascularization and the Remaining Area Supply of the Internal Carotid Artery Derivatives of the Red Kangaroo (Osphranter rufus)
Authors: Zdun Maciej , Ruszkowski Jakub Jędrzej , Gogulski Maciej
Published: August 2023

The red kangaroo (Osphranter rufus) is a member of Macropidideae superfamily. It is one of the four kangaroo species living nowadays, and it is the biggest one. It is native to Australia, where it is an abundant species living across the whole continent in stable populations. Outside its natural habit, the red kangaroo is a common species found in zoos and as patients in wildlife rehabilitation centers. Reports on kangaroo anatomy are scarce. Describing detailed anatomy is a base for establishing diagnostic and treatment protocols for different species of animals. Cardiovascular diseases and pathological changes suggestive of hypertension have been previously described in kangaroos. This creates a necessity for detailed studies on species’ vascular anatomy. New reports in the field of detailed vascular anatomy can bring considerable information that complements numerous studies on the evolution or biology of individual species. In this article, we describe the arterial vascularization of the brain and nearby regions of the cranial cavity using various anatomical techniques. The vascularization of the brain is discussed and compared with different mammalian species.
InDel and CNV within the AKAP13 Gene Revealing Strong Associations with Growth Traits in Goat
Authors: Song Xiaoyue , Bai Yangyang , Yuan Rongrong , Zhu Haijing , Lan Xianyong , Qu Lei
Published: August 2023

A-kinase-anchoring protein 13 (AKAP13) is a member of the AKAP protein family that has been found to be associated with bone formation. Thus, we investigated the AKAP13 gene as a potential candidate gene for molecular-marker-assisted selection (MAS) in breeding. Our aim was to explore genetic variations (InDel and CNV) within the AKAP13 gene of Shaanbei white cashmere (SBWC) goats and analyze their relationship with growth traits. Ultimately, we identified three InDel loci (16-bp deletion, 15-bp insertion, and 25-bp deletion) and three CNVs, and the 16-bp and 15-bp loci were significantly associated with goat body length (p < 0.05). Both the 16-bp deletion variant and the 15-bp insertion variant facilitated an increase in body length in goats. In addition to this, there was a certain superposition effect between 16-bp and 15-bp loci, although there was no linkage. Additionally, the CNV1 locus was significantly correlated with body height and body length of goats (p < 0.05), and CNV2 was significantly correlated with chest depth, chest circumference, and cannon circumference of goats (p < 0.05). Individuals with gain type showed excellent growth performance. In conclusion, the InDel and CNV loci that we have identified could possibly serve as effective molecular markers in goat breeding, which is very essential for improving efficiency and success of breeding. Moreover, our findings provide a new avenue for further research into the function of the AKAP13 gene.
Concepts on Accumulation of Pesticides and Veterinary Drugs in Fish: A Review with Emphasis in Tilapia
Authors: Jonsson Claudio Martín , de Queiroz Sonia Claudia do Nascimento
Published: August 2023

The quality of the aquatic environment can be compromised by the practice of intensive use of pesticides in agriculture and by the misuse of veterinary drugs. Therefore, organisms that live in aquatic ecosystems may be affected due to the presence of these chemicals, through runoff, leaching and other processes. Exposure of aquatic organisms to these xenobiotics could pose health risks. Consequently, there is a growing interest in predicting the bioaccumulation of these substances in aquatic biota from experiments conducted under laboratory conditions. Studies on fish have been performed due to its importance as human food and their wide distribution in most of the aquatic environment. Thus, this article reviews the concepts on determining the accumulation of pesticides and veterinary drugs in fish. The risk regarding the consumption of fish containing residues of these chemical agents, the acceptable daily intake, the testing protocols and the analytical techniques used to determine the residues of these substances in fish tissues are discussed. An emphasis on studies involving tilapia as the test organism was included because, according to Food and Agricultural Organization (FAO), this species is one of the most cultivated in the world.
Scorpion Venom as a Source of Antimicrobial Peptides: Overview of Biomolecule Separation, Analysis and Characterization Methods
Authors: Nasr Sara , Borges Adolfo , Sahyoun Christina , Nasr Riad , Roufayel Rabih , Legros Christian , Sabatier Jean-Marc , Fajloun Ziad
Published: August 2023

Scorpion venoms have long captivated scientific researchers, primarily due to the potency and specificity of the mechanism of action of their derived components. Among other molecules, these venoms contain highly active compounds, including antimicrobial peptides (AMPs) and ion channel-specific components that selectively target biological receptors with remarkable affinity. Some of these receptors have emerged as prime therapeutic targets for addressing various human pathologies, including cancer and infectious diseases, and have served as models for designing novel drugs. Consequently, extensive biochemical and proteomic investigations have focused on characterizing scorpion venoms. This review provides a comprehensive overview of the key methodologies used in the extraction, purification, analysis, and characterization of AMPs and other bioactive molecules present in scorpion venoms. Noteworthy techniques such as gel electrophoresis, reverse-phase high-performance liquid chromatography, size exclusion chromatography, and “omics” approaches are explored, along with various combinations of methods that enable bioassay-guided venom fractionation. Furthermore, this review presents four adapted proteomic workflows that lead to the comprehensive dissection of the scorpion venom proteome, with an emphasis on AMPs. These workflows differ based on whether the venom is pre-fractionated using separation techniques or is proteolytically digested directly before further proteomic analyses. Since the composition and functionality of scorpion venoms are species-specific, the selection and sequence of the techniques for venom analyses, including these workflows, should be tailored to the specific parameters of the study.
Low-Frequency Vibrations of Saccharides Using Terahertz Time-Domain Spectroscopy and Ab-Initio Simulations
Authors: Paraipan Andreea Aura , Luchetti Nicole , Mosca Conte Adriano , Pulci Olivia , Missori Mauro
Published: August 2023

The vibrational properties of molecular crystals in the terahertz range are controlled by the interplay of intermolecular and intramolecular interactions. The resulting delocalized normal modes span a substantial number of atoms within the unit cell of the molecular crystals and cannot be easily attributed to a specific stretching or bending mode. Several compounds were studied to understand the nature of normal modes. However, a systematic analysis of saccharides is still missing. This study investigates the terahertz vibrational properties of various saccharides, including glucose, galactose, lactose, cellobiose, and cellulose. The terahertz spectra were measured using terahertz time-domain spectroscopy. The samples were carefully characterized, and the residual Fabry–Perot oscillations were removed to obtain the absorption coefficient and refractive index of the saccharides. Density functional theory simulations were used to obtain theoretical terahertz spectra, considering hydrogen bonding interactions with an enhanced version of the van der Waals non-local density functional. The results revealed differences in low-energy vibrational modes frequencies, influenced by molecular structure properties, hydrogen bonding networks, and water content. Librations and internal vibrations were identified as dominant dynamics in the saccharides, with significant mixing between intermolecular and intramolecular vibrations. This comprehensive analysis sheds light on the vibrational behavior of saccharides in the terahertz range.
Controlling Composite TiO2 Powder Characteristics in the Solid-State Synthesis of BaTiO3 Powders for Improved Sintering and Permittivity: A Comparative Study
Authors: Lee Sang-Heun , Lee Yoon-Seok , Kim Jandi , Seo Ji-Hye , Cho Myunghee , Kwak Hun , Cheon Ran-Sae , Cho Seungchan , Kim Yangdo , Moon Kyoung-Seok , Choi Moonhee
Published: August 2023

In this study, the anatase–rutile phase fraction of TiO2, which would play a vital role in the solid-state synthesis of BaTiO3, was controlled to form a composite phase. The composite TiO2 was applied to a solid-state synthesis reaction, and the tetragonality, dielectric properties, and microstructure of the resulting BaTiO3 powders were analyzed under different synthesis conditions of TiO2. Furthermore, a comparative analysis of solid-state-synthesized BaTiO3 powders prepared using anatase, rutile, and mixed (anatase + rutile) TiO2 was performed to elucidate the mechanism of improvement in the sintering behavior and dielectric properties of the BaTiO3 powder synthesized using the composite TiO2. As a result of applying composite TiO2, BaTiO3 powder with excellent tetragonality was synthesized. In addition, it is effective in powder growth and the control of powder morphology, so sintering and dielectric properties can be improved at relatively low temperatures.
Research on a Super-Sub-Arc Bivariate Relative Angle Thermal Deformation Testing Method without Pitch Angle Limitation
Authors: Liu Yang , Xue Yaoke , Wang Hu , Pan Yue , Lin Shangmin , Ye Shuifu , Liu Jie
Published: August 2023

In light of the current situation where no testing equipment is available for measuring thermal deformation of objects, this paper proposes a novel method for accurate and precise measurement. The method overcomes the limitations of previous approaches that relied on pitch angle. By utilizing the principle of biplane multiple reflections, a bivariate laser spot displacement analysis algorithm is devised to attain highly precise measurements of bivariate angles. Additionally, a temperature gradient comparison algorithm is introduced to calculate the indicator test results under specific temperature conditions. To validate the effectiveness and reliability of this method, a testing system is constructed and utilized. The results demonstrate that the thermal deformation angle change test achieves an impressive accuracy of 0.015″ and a rate of thermal deformation angle change of 0.3247″/°C. These values are in close agreement with the previously simulated analysis result of 0.359″/°C, with only a relative error of 9.55%. Therefore, the test results confirm the efficacy and reliability of this testing method along with the feasibility of the algorithm processing.
Thermal Effect of the Back Radiation from Disk to Head after Laser Heating in HAMR
Authors: Zhao Yu , Zhang Guangyu
Published: August 2023

In a heat-assisted magnetic recording, the thermal effect of the head/disk interface has an important influence on the stability of the recording data. In this paper, we will discuss the thermal radiation from the disk, more specifically, the magnetic recording layer, which is at high temperature after laser heating, to the magnetic head, which is at room temperature. The radiative heat flux can be represented by the Poynting vector. In the near-field band, an effective way to obtain the electromagnetic fields is to solve the Maxwell’s equations combined with fluctuational electrodynamics. The near-field back radiation between specific head and disk material is calculated by the fluctuation-volume-current method. The radiative heat energy will induce the thermal deformation of the magnetic head, which will be discussed by the simulation, laying the foundation for adjusting and controlling the flying status.