bluuebunny/crossref_metadata_2025 huggingface dataset:
Postural Control during Progressively Increased Balance-Task Difficulty in Athletes with Unilateral Transfemoral Amputation: Effect of Ocular Mobility and Visuomotor Processing
Authors: Zwierko Michał , Lesiakowski Piotr , Zwierko Teresa
Published: August 2020

This study examined postural control during single leg stance test with progressively increased balance-task difficulty in soccer players with unilateral transfemoral amputation (n = 11) compared to able-bodied soccer players (n = 11). The overall stability index (OSI), the anterior/posterior stability index, and the medial/lateral stability index during three balance tasks with increasing surface instability were estimated. The oculomotor and visuomotor contribution to postural control in disabled athletes was analyzed. Oculomotor function, simple and choice reaction times, and peripheral perception were assessed in a series of visuomotor tests. The variation in OSI demonstrated significantly greater increases during postural tests with increased balance-task difficulty in the able-bodied soccer players compared to amputees (F(2,40) = 3.336, p < 0.05). Ocular mobility index correlated (p < 0.05) with OSI in conditions of increasing balance-task difficulty. Moreover, speed of eye-foot reaction has positive influence (p < 0.05) on stability indexes in tasks with an unstable surface. Amputee soccer players displayed comparable postural stability to able-bodied soccer players. Disabled athletes had better adaptability in restoring a state of balance in conditions of increased balance-task difficulty than the controls. The speed of visuomotor processing, characterized mainly by speed of eye-foot reaction, significantly contributed to these results.
Acceptability and Feasibility of HPV Self-Sampling as an Alternative Primary Cervical Cancer Screening in Under-Screened Population Groups: A Cross-Sectional Study
Authors: Wong Eliza Lai-Yi , Cheung Annie Wai-Ling , Wong Amy Yuen-Kwan , Chan Paul Kay-Sheung
Published: August 2020

Background: Cervical cancer is one of the most common cancers in women and about 90% of cervical cancer can be reduced by regular screening. The Pap smear has been well in place as a primary cervical screening method since 1950s; however, coverage is still not optimal. This study explored the feasibility of HPV self-sampling in two under-screened population groups in Hong Kong (HK): never screened and not regularly screened females, to estimate the uptake rate and preference rate in the future. Materials and Methods: This was a cross-sectional study to explore the acceptability and feasibility of HPV self-sampling in two age groups: aged 25–35 and aged ≥45, which were reported as the highest proportion of the under-screened population in HK between 2017 and 2018. The study invited eligible women from an HPV study cohort to perform HPV self-sampling at home by themselves. The number of specimens returned from participants was recorded and used to determine the feasibility of HPV self-sampling in the community. The participants were asked to fill in the questionnaires before and after HPV self-sampling to indicate their attitudes, acceptability, and future preference for HPV self-sampling as an acceptable alternative primary cervical cancer screening method. Results: A total of 177 subjects participated in the present study and have achieved a good overall uptake rate of 73% (129/177) who returned the self-collected cervicovaginal sample for HPV testing. Among the under-screened population, there was a higher response rate in aged ≥45 than those aged 25–35. The findings also revealed that women who were under-screened, including those who have never been screened, were more likely to prefer HPV self-sampling than those who had regular screening. This study found that the acceptability of HPV self-sampling was fairly positive among the respondents. The findings also indicated that HPV self-sampling was not only beneficial to enhance their health awareness but also to promote the cervical cancer screening uptake rate, especially among the under-screened or never screened populations. Conclusions: HPV self-sampling would be a solution to overcome the perceived barriers in clinician-based screening. The findings also indicated that it could be feasible to use as an alternative primary cervical cancer screening.
Retrograde Signaling: Understanding the Communication between Organelles
Authors: Mielecki Jakub , Gawroński Piotr , Karpiński Stanisław
Published: August 2020

Understanding how cell organelles and compartments communicate with each other has always been an important field of knowledge widely explored by many researchers. However, despite years of investigations, one point—and perhaps the only point that many agree on—is that our knowledge about cellular-signaling pathways still requires expanding. Chloroplasts and mitochondria (because of their primary functions in energy conversion) are important cellular sensors of environmental fluctuations and feedback they provide back to the nucleus is important for acclimatory responses. Under stressful conditions, it is important to manage cellular resources more efficiently in order to maintain a proper balance between development, growth and stress responses. For example, it can be achieved through regulation of nuclear and organellar gene expression. If plants are unable to adapt to stressful conditions, they will be unable to efficiently produce energy for growth and development—and ultimately die. In this review, we show the importance of retrograde signaling in stress responses, including the induction of cell death and in organelle biogenesis. The complexity of these pathways demonstrates how challenging it is to expand the existing knowledge. However, understanding this sophisticated communication may be important to develop new strategies of how to improve adaptability of plants in rapidly changing environments.
Tuning Adipogenic Differentiation in ADSCs by Metformin and Vitamin D: Involvement of miRNAs
Authors: Cruciani Sara , Garroni Giuseppe , Balzano Francesca , Pala Renzo , Bellu Emanuela , Cossu Maria Laura , Ginesu Giorgio Carlo , Ventura Carlo , Maioli Margherita
Published: August 2020

Fat tissue represents an important source of adipose-derived stem cells (ADSCs), which can differentiate towards several phenotypes under certain stimuli. Definite molecules as vitamin D are able to influence stem cell fate, acting on the expression of specific genes. In addition, miRNAs are important modulating factors in obesity and numerous diseases. We previously identified specific conditioned media able to commit stem cells towards defined cellular phenotypes. In the present paper, we aimed at evaluating the role of metformin on ADSCs differentiation. In particular, ADSCs were cultured in a specific adipogenic conditioned medium (MD), in the presence of metformin, alone or in combination with vitamin D. Our results showed that the combination of the two compounds is able to counteract the appearance of an adipogenic phenotype, indicating a feedforward regulation on vitamin D metabolism by metformin, acting on CYP27B1 and CYP3A4. We then evaluated the role of specific epigenetic modulating genes and miRNAs in controlling stem cell adipogenesis. The combination of the two molecules was able to influence stem cell fate, by modulating the adipogenic phenotype, suggesting their possible application in clinical practice in counteracting uncontrolled lipogenesis and obesity-related diseases.
Extended Exhaled Nitric Oxide Analysis in Interstitial Lung Diseases: A Systematic Review
Authors: Cameli Paolo , Bargagli Elena , Bergantini Laura , d’Alessandro Miriana , Pieroni Maria , Fontana Giovanni A. , Sestini Piersante , Refini Rosa Metella
Published: August 2020

Fractional exhaled nitric oxide (FeNO) is a well-known and widely accepted biomarker of airways inflammation that can be useful in the therapeutic management, and adherence to inhalation therapy control, in asthmatic patients. However, the multiple-flows assessment of FeNO can provide a reliable measurement of bronchial and alveolar production of NO, supporting its potential value as biomarker also in peripheral lung diseases, such as interstitial lung diseases (ILD). In this review, we first discuss the role of NO in the pathobiology of lung fibrosis and the technique currently approved for the measurement of maximum bronchial flux of NO (J’awNO) and alveolar concentration of NO (CaNO). We systematically report the published evidence regarding extended FeNO analysis in the management of patients with different ILDs, focusing on its potential role in differential diagnosis, prognostic evaluation and severity assessment of disease. The few available data concerning extended FeNO analysis, and the most common comorbidities of ILD, are explored too. In conclusion, multiple-flows FeNO analysis, and CaNO in particular, appears to be a promising tool to be implemented in the diagnostic and prognostic pathways of patients affected with ILDs.
Characterization of the Heavy-Metal-Associated Isoprenylated Plant Protein (HIPP) Gene Family from Triticeae Species
Authors: Zhang Heng , Zhang Xu , Liu Jia , Niu Ying , Chen Yiming , Hao Yongli , Zhao Jia , Sun Li , Wang Haiyan , Xiao Jin , Wang Xiue
Published: August 2020

Heavy-metal-associated (HMA) isoprenylated plant proteins (HIPPs) only exist in vascular plants. They play important roles in responses to biotic/abiotic stresses, heavy-metal homeostasis, and detoxification. However, research on the distribution, diversification, and function of HIPPs in Triticeae species is limited. In this study, a total of 278 HIPPs were identified from a database from five Triticeae species, and 13 were cloned from Haynaldia villosa. These genes were classified into five groups by phylogenetic analysis. Most HIPPs had one HMA domain, while 51 from Clade I had two, and all HIPPs had good collinear relationships between species or subgenomes. In silico expression profiling revealed that 44 of the 114 wheat HIPPs were dominantly expressed in roots, 43 were upregulated under biotic stresses, and 29 were upregulated upon drought or heat treatment. Subcellular localization analysis of the cloned HIPPs from H. villosa showed that they were expressed on the plasma membrane. HIPP1-V was upregulated in H. villosa after Cd treatment, and transgenic wheat plants overexpressing HIPP1-V showed enhanced Cd tolerance, as shown by the recovery of seed-germination and root-growth inhibition by supplementary Cd. This research provides a genome-wide overview of the Triticeae HIPP genes and proved that HIPP1-V positively regulates Cd tolerance in common wheat.
Knockout of stim2a Increases Calcium Oscillations in Neurons and Induces Hyperactive-Like Phenotype in Zebrafish Larvae
Authors: Gupta Rishikesh Kumar , Wasilewska Iga , Palchevska Oksana , Kuźnicki Jacek
Published: August 2020

Stromal interaction molecule (STIM) proteins play a crucial role in store-operated calcium entry (SOCE) as endoplasmic reticulum Ca2+ sensors. In neurons, STIM2 was shown to have distinct functions from STIM1. However, its role in brain activity and behavior was not fully elucidated. The present study analyzed behavior in zebrafish (Danio rerio) that lacked stim2a. The mutant animals had no morphological abnormalities and were fertile. RNA-sequencing revealed alterations of the expression of transcription factor genes and several members of the calcium toolkit. Neuronal Ca2+ activity was measured in vivo in neurons that expressed the GCaMP5G sensor. Optic tectum neurons in stim2a−/− fish had more frequent Ca2+ signal oscillations compared with neurons in wildtype (WT) fish. We detected an increase in activity during the visual–motor response test, an increase in thigmotaxis in the open field test, and the disruption of phototaxis in the dark/light preference test in stim2a−/− mutants compared with WT. Both groups of animals reacted to glutamate and pentylenetetrazol with an increase in activity during the visual–motor response test, with no major differences between groups. Altogether, our results suggest that the hyperactive-like phenotype of stim2a−/− mutant zebrafish is caused by the dysregulation of Ca2+ homeostasis and signaling.
Fock-Space Coupled Cluster Theory: Systematic Study of Partial Fourth Order Triples Schemes for Ionization Potential and Comparison with Bondonic Formalism
Authors: Basumallick Suhita , Pal Sourav , Putz Mihai V.
Published: August 2020

In this paper, we have made a systematic study of partial fourth order perturbative schemes due to triples to compute the ionization potential within Fock-space multi-reference coupled-cluster theory. In particular, we have obtained computationally less expensive correlation schemes due to fourth order triples. Prototype examples have been considered to explore the efficacy of the approximate methods mentioned, while the bondonic formalism supporting the bonding phenomenology is also respectively for the first time here advanced.
MmpL3 Inhibition: A New Approach to Treat Nontuberculous Mycobacterial Infections
Authors: Sethiya Jigar P. , Sowards Melanie A. , Jackson Mary , North Elton Jeffrey
Published: August 2020

Outside of Mycobacterium tuberculosis and Mycobacterium leprae, nontuberculous mycobacteria (NTM) are environmental mycobacteria (>190 species) and are classified as slow- or rapid-growing mycobacteria. Infections caused by NTM show an increased incidence in immunocompromised patients and patients with underlying structural lung disease. The true global prevalence of NTM infections remains unknown because many countries do not require mandatory reporting of the infection. This is coupled with a challenging diagnosis and identification of the species. Current therapies for treatment of NTM infections require multidrug regimens for a minimum of 18 months and are associated with serious adverse reactions, infection relapse, and high reinfection rates, necessitating discovery of novel antimycobacterial agents. Robust drug discovery processes have discovered inhibitors targeting mycobacterial membrane protein large 3 (MmpL3), a protein responsible for translocating mycolic acids from the inner membrane to periplasm in the biosynthesis of the mycobacterial cell membrane. This review focuses on promising new chemical scaffolds that inhibit MmpL3 function and represent interesting and promising putative drug candidates for the treatment of NTM infections. Additionally, agents (FS-1, SMARt-420, C10) that promote reversion of drug resistance are also reviewed.
P2 Receptors Influence hMSCs Differentiation towards Endothelial Cell and Smooth Muscle Cell Lineages
Authors: Zhang Yu , Babczyk Patrick , Pansky Andreas , Kassack Matthias Ulrich , Tobiasch Edda
Published: August 2020

Background: Human mesenchymal stem cells (hMSCs) have shown their multipotential including differentiating towards endothelial and smooth muscle cell lineages, which triggers a new interest for using hMSCs as a putative source for cardiovascular regenerative medicine. Our recent publication has shown for the first time that purinergic 2 receptors are key players during hMSC differentiation towards adipocytes and osteoblasts. Purinergic 2 receptors play an important role in cardiovascular function when they bind to extracellular nucleotides. In this study, the possible functional role of purinergic 2 receptors during MSC endothelial and smooth muscle differentiation was investigated. Methods and Results: Human MSCs were isolated from liposuction materials. Then, endothelial and smooth muscle-like cells were differentiated and characterized by specific markers via Reverse Transcriptase-PCR (RT-PCR), Western blot and immunochemical stainings. Interestingly, some purinergic 2 receptor subtypes were found to be differently regulated during these specific lineage commitments: P2Y4 and P2Y14 were involved in the early stage commitment while P2Y1 was the key player in controlling MSC differentiation towards either endothelial or smooth muscle cells. The administration of natural and artificial purinergic 2 receptor agonists and antagonists had a direct influence on these differentiations. Moreover, a feedback loop via exogenous extracellular nucleotides on these particular differentiations was shown by apyrase digest. Conclusions: Purinergic 2 receptors play a crucial role during the differentiation towards endothelial and smooth muscle cell lineages. Some highly selective and potent artificial purinergic 2 ligands can control hMSC differentiation, which might improve the use of adult stem cells in cardiovascular tissue engineering in the future.
Organoids in Translational Oncology
Authors: Tatullo Marco , Marrelli Benedetta , Benincasa Caterina , Aiello Elisabetta , Makeeva Irina , Zavan Barbara , Ballini Andrea , De Vito Danila , Spagnuolo Gianrico
Published: August 2020

Translational medicine aims to translate the most promising preclinical research into clinical practice. Oncology is a continuously growing medical field: the scientific research on cancer biology is currently based on in vitro experiments, carried out on tissue culture plates (TCPs) and other 2D samples. In this context, 3D printing has greatly improved the biofabrication of new biological matrices that mimic the extracellular environments, which may characterize healthy from cancerous tissues. Organoids have recently been described in several reports on scientific literature. The term that better describes such organoids-based tumoral tissues is “tumoroids”. Tumoroids are substantially “tumor-like organoids”, typically deriving from primary tumors harvested from patients. This topical review aims to give an update on organoids applied in translational medicine, paying specific attention to their use in the investigation of the main molecular mechanisms of cancer onset and growth, and on the most impacting strategies for effective targeted therapies.
Impact of PD-L1 Scores and Changes on Clinical Outcome in Rectal Cancer Patients Undergoing Neoadjuvant Chemoradiotherapy
Authors: Huemer Florian , Klieser Eckhard , Neureiter Daniel , Schlintl Verena , Rinnerthaler Gabriel , Pagès Franck , Kirilovsky Amos , El Sissy Carine , Iglseder Wolfgang , Singhartinger Franz , Jäger Tarkan , Dinnewitzer Adam , Zaborsky Nadja , Steiner Markus , Greil Richard , Weiss Lukas
Published: August 2020

Reports on the prognostic role of programmed death-ligand 1 (PD-L1) expression in rectal cancer are controversial. We investigated expression patterns and changes of PD-L1 in rectal cancer patients undergoing neoadjuvant chemoradiotherapy (CRT). Seventy-two patients diagnosed with rectal cancer and/or treated with fluorouracil-based neoadjuvant CRT at the Department of Internal Medicine III of the Paracelsus Medical University Salzburg (Austria) between January 2003 and October 2012 were included. PD-L1 scoring was performed according to the tumor proportion score (TPS), combined positive score (CPS), and immune cell score (IC). PD-L1 TPS prior to neoadjuvant CRT had a statistically significant impact on survival (median: ≤1%: 95.4 months (95% CI: 51.8—not reached) vs. >1%: not reached, p = 0.03, log-rank). Patients with a PD-L1 TPS ≤1% prior to and after CRT showed an inferior survival compared to all other patients (median: 56.7 months (95% CI: 51.4—not reached) vs. not reached, p = 0.005, log-rank). In multivariate analysis, PD-L1 TPS prior to neoadjuvant CRT (>1% vs. ≤1%, hazard ratio: 0.29 (95% CI: 0.11–0.76), p = 0.01) remained independently associated with survival. In conclusion, low PD-L1 TPS was associated with inferior survival in rectal cancer patients undergoing neoadjuvant CRT. A prospective validation of the prognostic value of PD-L1 expression in rectal cancer patients within a clinical trial is necessitated.
Can Voice Pitch Be Preserved in Patients after Transoral Endoscopic Thyroidectomy Vestibular Approach?
Authors: Kim Mi Ra , Park Yeong Jun , Park Byung Whoo , Suh Taekyung , Kim Sang-Yeon , Moon Tae Hoon , Lee Dong Hyun , Park Jun-Ook
Published: August 2020

Introduction: Transoral endoscopic thyroidectomy vestibular approach (TOETVA) has become increasingly popular. Several reports have emphasized the safety and efficacy of this new approach. However, there is no report on functional voice outcomes, including voice pitch change after TOETVA. Methods: The functional voice outcomes of patients undergoing TOETVA were compared with those of patients undergoing conventional thyroidectomy. A total of 82 consecutive patients were included in the study: 44 underwent thyroid lobectomy via TOETVA (transoral group) and 38 underwent thyroid lobectomy via the classic cervical approach (open group). Thyroidectomy-related voice questionnaire (TVQ), perceptual voice analysis, fiberoptic laryngoscopic and videolaryngostroboscopic examinations, and acoustic analysis were carried out before and one month after surgery. The changes in these values after surgery and the differences between the transoral and open groups were analyzed. Results: We found no significant postoperative change in voice workups in either group. The mean high pitch decreased (from 367.91 ± 120.98 to 325.80 ± 100.86 Hz, p = 0.069) in the transoral group, but statistical significance was not attained. Clinically significant changes in pitch (postoperative change in speaking fundamental frequency, ΔSFF ≥ 12) after surgery were evident in seven (15.91%) patients in the transoral group and eight (21.05%) patients in the open group without significant difference (p = 0.579). Conclusions: This is the first study to assess functional voice outcomes (including pitch) after TOETVA compared with conventional open surgery. TOETVA was associated with good voice outcomes without any significant drop in pitch.
Clinical Application of Forced Oscillation Technique (FOT) in Early Detection of Airway Changes in Smokers
Authors: Bhattarai Prem , Myers Stephen , Chia Collin , Weber Heinrich C. , Young Sally , Williams Andrew D. , Sohal Sukhwinder Singh
Published: August 2020

The forced oscillation technique (FOT) is a non-invasive method to assess airway function by emitting oscillatory signals into the respiratory tract during tidal ventilation. This opinion piece discusses the current use, trialled modification and future directions in utilizing FOT as a novel diagnostic tool for early detection of small airway changes in smokers. The published evidence to date has shown that FOT parameters could be a sensitive diagnostic tool to detect early respiratory changes in smokers. Multiple frequencies and the frequency dependence of resistance and reactance can provide the most valuable and early information regarding smoking induced changes in airways. Considering its non-invasiveness, lower level of discomfort to patients than spirometry, feasibility, and cost effectiveness, it could be the first-choice diagnostic technique for detection of early respiratory changes in smokers. The finding of FOT could further be supported and correlated with inflammatory markers.
Effects of Dehydration on Archery Performance, Subjective Feelings and Heart Rate during a Competition Simulation
Authors: Savvides Alexandros , D. Giannaki Christoforos , Vlahoyiannis Angelos , S. Stavrinou Pinelopi , Aphamis George
Published: August 2020

This study aimed to investigate the effect of dehydration on archery performance, subjective feelings and heart rate response. Ten national level archers performed two archery competition simulations, once under euhydration (EUH) and once in a dehydrated state (DEH), induced by 24-h reduced fluid intake. Hydration status was verified prior to each trial by urine specific gravity (USG ≥ 1.025). Archery score was measured according to official archery regulations. Subjective feelings of thirst, fatigue and concentration were recorded on a visual analogue scale. Heart rate was continuously monitored during the trials. Archery performance was similar between trials (p = 0.155). During DEH trial (USG 1.032 ± 0.005), the athletes felt thirstier (p < 0.001), more fatigued (p = 0.041) and less able to concentrate (p = 0.016) compared with the EUH trial (USG 1.015 ± 0.004). Heart rate during DEH at baseline (85 ± 5 b∙min−1) was higher (p = 0.021) compared with EUH (78 ± 6 b∙min−1) and remained significantly higher during the latter stages of the DEH compared to EUH trial. In conclusion, archery performance over 72 arrows was not affected by dehydration, despite the induced psychological and physiological strain, revealed from decreased feeling of concentration, increased sensation of fatigue and increased heart rate during the DEH trial.
Realistic Dynamic Numerical Phantom for MRI of the Upper Vocal Tract
Authors: Martin Joe , Ruthven Matthieu , Boubertakh Redha , Miquel Marc E.
Published: August 2020

Dynamic and real-time MRI (rtMRI) of human speech is an active field of research, with interest from both the linguistics and clinical communities. At present, different research groups are investigating a range of rtMRI acquisition and reconstruction approaches to visualise the speech organs. Similar to other moving organs, it is difficult to create a physical phantom of the speech organs to optimise these approaches; therefore, the optimisation requires extensive scanner access and imaging of volunteers. As previously demonstrated in cardiac imaging, realistic numerical phantoms can be useful tools for optimising rtMRI approaches and reduce reliance on scanner access and imaging volunteers. However, currently, no such speech rtMRI phantom exists. In this work, a numerical phantom for optimising speech rtMRI approaches was developed and tested on different reconstruction schemes. The novel phantom comprised a dynamic image series and corresponding k-space data of a single mid-sagittal slice with a temporal resolution of 30 frames per second (fps). The phantom was developed based on images of a volunteer acquired at a frame rate of 10 fps. The creation of the numerical phantom involved the following steps: image acquisition, image enhancement, segmentation, mask optimisation, through-time and spatial interpolation and finally the derived k-space phantom. The phantom was used to: (1) test different k-space sampling schemes (Cartesian, radial and spiral); (2) create lower frame rate acquisitions by simulating segmented k-space acquisitions; (3) simulate parallel imaging reconstructions (SENSE and GRAPPA). This demonstrated how such a numerical phantom could be used to optimise images and test multiple sampling strategies without extensive scanner access.
Life Cycle Assessment of Alternative Ship Fuels for Coastal Ferry Operating in Republic of Korea
Authors: Hwang Sang Soo , Gil Sung Jin , Lee Gang Nam , Lee Ji Won , Park Hyun , Jung Kwang Hyo , Suh Sung Bu
Published: August 2020

In this study, the environmental impacts of various alternative ship fuels for a coastal ferry were assessed by the life cycle assessment (LCA) analysis. The comparative study was performed with marine gas oil (MGO), natural gas, and hydrogen with various energy sources for a 12,000 gross tonne (GT) coastal ferry operating in the Republic of Korea (ROK). Considering the energy imports of ROK, i.e., MGO from Saudi Arabia and natural gas from Qatar, these countries were chosen to provide the MGO and the natural gas for the LCA. The hydrogen is considered to be produced by steam methane reforming (SMR) from natural gas with hard coal, nuclear energy, renewable energy, and electricity in the ROK model. The lifecycles of the fuels were analyzed in classifications of Well-to- Tank, Tank-to-Wake, and Well-to-Wake phases. The environmental impacts were provided in terms of global warming potential (GWP), acidification potential (AP), photochemical potential (POCP), eutrophication potential (EP), and particulate matter (PM). The results showed that MGO and natural gas cannot be used for ships to meet the International Maritime Organization’s (IMO) 2050 GHG regulation. Moreover, it was pointed out that the energy sources in SMR are important contributing factors to emission levels. The paper concludes with suggestions for a hydrogen application plan for ships from small, nearshore ships in order to truly achieve a ship with zero emissions based on the results of this study.
Effects of Glyphosate-Based and Derived Products on Sea Urchin Larval Development
Authors: Asnicar Davide , Cappelli Costanza , Sallehuddin Ahmad Safuan , Maznan Nur Atiqah , Marin Maria Gabriella
Published: August 2020

Despite the widespread use of herbicide glyphosate in cultivation, its extensive runoff into rivers and to coastal areas, and the persistence of this chemical and its main degradation product (aminomethylphosphonic acid, AMPA) in the environment, there is still little information on the potential negative effects of glyphosate, its commercial formulation Roundup® and AMPA on marine species. This study was conducted with the aim of providing a comparative evaluation of the effects of glyphosate-based and its derived chemicals on the larval development of the sea urchin Paracentrotus lividus, thus providing new data to describe the potential ecotoxicity of these contaminants. In particular, the effects on larval development, growth and metabolism were assessed during 48 h of exposure from the time of egg fertilization. The results confirm that AMPA and its parent compound, glyphosate have similar toxicity, as observed in other marine invertebrates. However, interestingly, the Roundup® formulation seemed to be less toxic than the glyphosate alone.
Cardiovascular System in COVID-19: Simply a Viewer or a Leading Actor?
Authors: Mottola Filiberto , Verde Nicoletta , Ricciolino Riccardo , Di Mauro Marco , Migliaccio Marco , Carfora Vincenzo , Spiniello Giorgio , Coppola Nicola ,   Vanvitelli COVID-19 Group
Published: August 2020

As of January 2020, a new pandemic has spread from Wuhan and caused thousands of deaths worldwide. Several studies have observed a relationship between coronavirus disease (COVID-19) infection and the cardiovascular system with the appearance of myocardial damage, myocarditis, pericarditis, heart failure and various arrhythmic manifestations, as well as an increase in thromboembolic risk. Cardiovascular manifestations have been highlighted especially in older and more fragile patients and in those with multiple cardiovascular risk factors such as cancer, diabetes, obesity and hypertension. In this review, we will examine the cardiac involvement associated with SARS-CoV-2 infection, focusing on the pathophysiological mechanism underlying manifestations and their clinical implication, taking into account the main scientific papers published to date.
Biomechanics of the Human Middle Ear with Viscoelasticity of the Maxwell and the Kelvin–Voigt Type and Relaxation Effect
Authors: Rusinek Rafal , Szymanski Marcin , Zablotni Robert
Published: August 2020

The middle ear is one of the smallest biomechanical systems in the human body and is responsible for the hearing process. Hearing is modelled in different ways and by various methods. In this paper, three-degree-of-freedom models of the human middle ear with different viscoelastic properties are proposed. Model 1 uses the Maxwell type viscoelasticity, Model 2 is based on the Kelvin–Voigt viscoelasticity, and Model 3 uses the Kelvin–Voigt viscoelasticity with relaxation effect. The primary aim of the study is to compare the models and their dynamic responses to a voice excitation. The novelty of this study lies in using different models of viscoelasticity and relaxation effect that has been previously unstudied. First, mathematical models of the middle ear were built, then they were solved numerically by the Runge–Kutta procedure and finally, numerical results were compared with those obtained from experiments carried out on the temporal bone with the Laser Doppler Vibrometer. The models exhibit differences in the natural frequency and amplitudes near the second resonance. All analysed models can be used for modelling the rapidly changing processes that occur in the ear and to control active middle ear implants.
Dry Cold Forging of Pure Titanium Wire to Thin Plate with Use of β-SiC Coating Dies
Authors: Aizawa Tatsuhiko , Yoshino Tomoaki , Fukuda Tatsuya , Shiratori Tomomi
Published: August 2020

Dense β-SiC coating with 3C-structure was utilized as a dry cold forging punch and core-die. Pure titanium T328H wires of industrial grade II were employed as a work material. No adhesion or galling of metallic titanium was detected on the contact interface between this β-SiC die and titanium work, even after this continuous forging process, up to a reduction in thickness by 70%. SEM (Scanning Electron Microscopy) and EDX (Electron Dispersive X-ray spectroscopy) were utilized to analyze this contact interface. A very thin titanium oxide layer was in situ formed in the radial direction from the center of the contact interface. Isolated carbon from β-SiC agglomerated and distributed in dots at the center of the initial contact interface. Raman spectroscopy was utilized, yielding the discovery that this carbon is unbound as a free carbon or not bound in SiC or TiC and that intermediate titanium oxides are formed with TiO2 as a tribofilm.
A Review of Flexible Wearable Antenna Sensors: Design, Fabrication Methods, and Applications
Authors: El Gharbi Mariam , Fernández-García Raúl , Ahyoud Saida , Gil Ignacio
Published: August 2020

This review paper summarizes various approaches developed in the literature for antenna sensors with an emphasis on flexible solutions. The survey helps to recognize the limitations and advantages of this technology. Furthermore, it offers an overview of the main points for the development and design of flexible antenna sensors from the selection of the materials to the framing of the antenna including the different scenario applications. With regard to wearable antenna sensors deployment, a review of the textile materials that have been employed is also presented. Several examples related to human body applications of flexible antenna sensors such as the detection of NaCl and sugar solutions, blood and bodily variables such as temperature, strain, and finger postures are also presented. Future investigation directions and research challenges are proposed.
A Prediction Model on Viscoelastic Fatigue Damage of Asphalt Mixture
Authors: Li Limin , Jiang Xiaoling , Lin Yuliang , Yan Heng
Published: August 2020

Fatigue damage affects both durability and safety, and it has been the most important distress in asphalt concrete. Fatigue damage occurs as a result of repeated traffic loading. An asphalt mixture is a typical viscoelastic material, and its fatigue damage is related to its viscoelastic properties. Under repeated traffic loading, the combined effects of creep damage and fatigue damage will shorten its fatigue life. Currently, the evaluation of the fatigue damage of asphalt mixtures rarely considers the combined effects of creep damage and fatigue damage. To solve this problem, a viscoelastic fatigue damage prediction model of an asphalt mixture considering the combined effects of creep damage and fatigue damage is put forward by introducing parameter β and a displacement factor based on theoretical derivations and testing. The results show that the model can embody the viscoelastic fatigue damage essence of asphalt mixtures, and it can also consider the effects of aging degree, temperature, load frequency and stress on fatigue damage of asphalt mixtures. The maximum relative error of the testing and prediction results of fatigue life is 0.15, and it is a reasonable prediction model.
A Comparative Study of the Fractional-Order Clock Chemical Model
Authors: Srivastava Hari Mohan , Saad Khaled M.
Published: August 2020

In this paper, a comparative study has been made between different algorithms to find the numerical solutions of the fractional-order clock chemical model (FOCCM). The spectral collocation method (SCM) with the shifted Legendre polynomials, the two-stage fractional Runge–Kutta method (TSFRK) and the four-stage fractional Runge–Kutta method (FSFRK) are used to approximate the numerical solutions of FOCCM. Our results are compared with the results obtained for the numerical solutions that are based upon the fundamental theorem of fractional calculus as well as the Lagrange polynomial interpolation (LPI). Firstly, the accuracy of the results is checked by computing the absolute error between the numerical solutions by using SCM, TSFRK, FSFRK, and LPI and the exact solution in the case of the fractional-order logistic equation (FOLE). The numerical results demonstrate the accuracy of the proposed method. It is observed that the FSFRK is better than those by SCM, TSFRK and LPI in the case of an integer order. However, the non-integer orders in the cases of the SCM and LPI are better than those obtained by using the TSFRK and FSFRK. Secondly, the absolute error between the numerical solutions of FOCCM based upon SCM, TSFFRK, FSFRK, and LPI for integer order and non-integer order has been computed. The absolute error in the case of the integer order by using the three methods of the third order is considered. For the non-integer order, the order of the absolute error in the case of SCM is found to be the best. Finally, these results are graphically illustrated by means of different figures.
Dominating the Direct Product of Two Graphs through Total Roman Strategies
Authors: Martínez Abel Cabrera , Kuziak Dorota , Peterin Iztok , Yero Ismael G.
Published: August 2020

Given a graph G without isolated vertices, a total Roman dominating function for G is a function f:V(G)→{0,1,2} such that every vertex u with f(u)=0 is adjacent to a vertex v with f(v)=2, and the set of vertices with positive labels induces a graph of minimum degree at least one. The total Roman domination number γtR(G) of G is the smallest possible value of ∑v∈V(G)f(v) among all total Roman dominating functions f. The total Roman domination number of the direct product G×H of the graphs G and H is studied in this work. Specifically, several relationships, in the shape of upper and lower bounds, between γtR(G×H) and some classical domination parameters for the factors are given. Characterizations of the direct product graphs G×H achieving small values (≤7) for γtR(G×H) are presented, and exact values for γtR(G×H) are deduced, while considering various specific direct product classes.
The Convergence Analysis of a Numerical Method for a Structured Consumer-Resource Model with Delay in the Resource Evolution Rate
Authors: Abia Luis M. , Angulo Óscar , López-Marcos Juan C. , López-Marcos Miguel A.
Published: August 2020

In this paper, we go through the development of a new numerical method to obtain the solution to a size-structured population model that describes the evolution of a consumer feeding on a dynamical resource that reacts to the environment with a lag-time response. The problem involves the coupling of the partial differential equation that represents the population evolution and an ordinary differential equation with a constant delay that describes the evolution of the resource. The numerical treatment of this problem has not been considered before when a delay is included in the resource evolution rate. We analyzed the numerical scheme and proved a second-order rate of convergence by assuming enough regularity of the solution. We numerically confirmed the theoretical results with an academic test problem.
New Tricks with an Old Sponge: Feature-Based Molecular Networking Led to Fast Identification of New Stylissamide L from Stylissa caribica
Authors: Scarpato Silvia , Teta Roberta , Della Sala Gerardo , Pawlik Joseph R. , Costantino Valeria , Mangoni Alfonso
Published: August 2020

Feature-based molecular networking was used to re-examine the secondary metabolites in extracts of a very well studied marine sponge, Stylissa caribica, known to contain a large array of cyclic peptides and brominated alkaloids. The analysis revealed the presence of 13 cyclic peptides in the sponge that had never been detected in previous work and appeared to be new compounds. The most abundant one was isolated and shown to be a new proline-rich cyclic heptapetide that was called stylissamide L (1). Structure of compound 1, including the cis/trans geometry of the three proline residues, was determined by extensive NMR studies; the l configuration of the seven amino acid residues was determined using Marfey’s method. Stylissamide L was tested for activity as a cell growth inhibitor and cell migration inhibitor on two cancer cell lines but, unlike other members of the stylissamide family, it showed no significant activity. This approach showed that even a thoroughly studied species such as S. caribica may contain new chemistry that can be revealed if studied with the right tools.
Eighteen New Aeruginosamide Variants Produced by the Baltic Cyanobacterium Limnoraphis CCNP1324
Authors: Cegłowska Marta , Szubert Karolia , Wieczerzak Ewa , Kosakowska Alicja , Mazur-Marzec Hanna
Published: August 2020

Cyanobactins are a large family of ribosomally synthesized and post-translationally modified cyanopeptides (RiPPs). Thus far, over a hundred cyanobactins have been detected in different free-living and symbiotic cyanobacteria. The majority of these peptides have a cyclic structure. The occurrence of linear cyanobactins, aeruginosamides and virenamide, has been reported sporadically and in few cyanobacterial taxa. In the current work, the production of cyanobactins by Limnoraphis sp. CCNP1324, isolated from the brackish water Baltic Sea, has been studied for the first time. In the strain, eighteen new aeruginosamide (AEG) variants have been detected. These compounds are characterized by the presence of prenyl and thiazole groups. A common element of AEGs produced by Limnoraphis sp. CCNP1324 is the sequence of the three C-terminal residues containing proline, pyrrolidine and methyl ester of thiazolidyne-4-carboxylic acid (Pro-Pyr-TzlCOOMe) or thiazolidyne-4-carboxylic acid (Pro-Pyr-TzlCOOH). The aeruginosamides with methylhomotyrosine (MeHTyr1) and with the unidentified N-terminal amino acids showed strong cytotoxic activity against human breast cancer cells (T47D).
Complement Properdin Determines Disease Activity in MRL/lpr Mice
Authors: Alaridhee Hasanain , Alharbi Azzah , Saeed Zeayd , Thomas Róisín C. , Stover Cordula M.
Published: August 2020

Background and objects: In systemic lupus erythematosus, circulating immune complexes activate complement and, when trapped in renal capillaries, cause glomerulonephritis. Mouse models have been used in the preclinical assessment of targeting complement activation pathways to manage chronic inflammation in lupus. Properdin is the only known positive regulator of complement activation, but its role in the severity of lupus nephritis has not been studied yet. Materials and Methods: Fully characterized properdin-deficient mice were crossed with lupus prone MRL/lpr mice on C57Bl/6 background. Results: Compared to MRL/lpr properdin wildtype mice, MRL/lpr properdin-deficient mice had significantly lower anti-DNA antibody titres, TNFα and BAFF levels in serum. The qualitative glomerulonephritic score was less severe and there was significantly less serum creatinine in MRL/lpr properdin-deficient mice compared to MRL/lpr properdin wildtype littermate mice. Conclusion: Properdin plays a significant role in the severity of lupus overall and specifically in the extent of glomerulonephritis observed in MRL/lpr mice. Because MRL/lpr properdin-deficient mice had lower levels of anti-DNA antibodies, inflammatory mediators and markers of renal impairment, the study implies that properdin could constitute a novel therapy target in lupus disease.
Long-Term Prognostic Significance of High-Sensitive Troponin I Increase during Hospital Stay in Patients with Acute Myocardial Infarction and Non-Obstructive Coronary Arteries
Authors: Jędrychowska Magdalena , Januszek Rafał , Wańha Wojciech , Malinowski Krzysztof Piotr , Kunik Piotr , Trznadel Agata , Bartuś Joanna , Staszczak Bartłomiej , Januszek Sławomir Mateusz , Kameczura Tomasz , Wojakowski Wojciech , Surdacki Andrzej , Bartuś Stanisław
Published: August 2020

Background and Objectives: A topic already widely investigated is the negative prognostic value regarding the extent of high sensitive troponin I (hs-TnI) increases among patients with myocardial infarction (MI) and obstructive coronary atherosclerosis compared to a group of patients with MI and non-obstructive coronary atherosclerosis (MINOCA). Thus, the aim of this study was to evaluate the prognostic value concerning the extent of hs-TnI increase on clinical outcomes among patients with a MINOCA working diagnosis. Materials and Methods: We selected 337 consecutive patients admitted to hospital with a working diagnosis of MINOCA. The patients were divided in three groups according to the extent of hs-TnI increase during hospitalization (increase ≤5-times above the limit of the upper norm, >5 and ≤20-times, and >20-times). The study endpoints included all-cause mortality and major adverse cardiac and cerebrovascular events (MACCE; cerebral stroke and transient ischemic attacks, MI, coronary artery revascularization, either percutaneous coronary intervention or coronary artery bypass grafting and all-cause mortality). Results: During the mean follow-up period of 516.1 ± 239.8 days, using Kaplan–Meier survival curve analysis, significantly higher mortality rates were demonstrated among patients from the group with the greatest hs-TnI increase compared to the remaining groups (p = 0.01) and borderline values for MACCE (p = 0.053). Multivariable cox regression analysis did not confirm hs-TnI among factors related to increased MACCE or all-cause mortality rates. Conclusion: While a relationship between clinical outcomes and the extent of the hs-TnI increase among patients with a MINOCA working diagnosis remains, it does not seem to be not as strong as it is in patients with obstructive coronary atherosclerosis.
Effect of Number of Variants of Zirconium Hydride on Grain Growth of Zirconium
Authors: Yoon Bohyun , Chang Kunok
Published: August 2020

The microstructure characteristics of Zr-hydride in Zr are important concerns in metallurgy and nuclear engineering. In particular, it is known that the correlation between hydride and the grain boundary microstructure has a great influence on properties. In this study, a phase-field model was used to evaluate evolutions of the fractions of intra-granular and inter-granular hydride and multi-contacted hydride according to the number of structural variants of δ-hydride in the 3D system. The effect of the numbers of crystallographic variants of hydride on grain growth kinetics was also analyzed. We found that the pinning effect in 3D is minimized when hydrides have one crystallographic variant, which is contradictory observation with the 2D case. With grain structures with comparable average grain radii and quantities, we found that the fraction of the intra-granular and inter-granular hydrides increase as the number of crystallographic variants increases.