bluuebunny/crossref_metadata_2025 huggingface dataset:
Influence of the HDAC Inhibitor Valproic Acid on the Growth and Proliferation of Temsirolimus-Resistant Prostate Cancer Cells In Vitro
Authors: Makarević Jasmina , Rutz Jochen , Juengel Eva , Maxeiner Sebastian , Tsaur Igor , Chun Felix K.-H. , Bereiter-Hahn Jürgen , Blaheta Roman A.
Published: April 2019

The mechanistic target of rapamycin (mTOR) is elevated in prostate cancer, making this protein attractive for tumor treatment. Unfortunately, resistance towards mTOR inhibitors develops and the tumor becomes reactivated. We determined whether epigenetic modulation by the histone deacetylase (HDAC) inhibitor, valproic acid (VPA), may counteract non-responsiveness to the mTOR inhibitor, temsirolimus, in prostate cancer (PCa) cells. Prostate cancer cells, sensitive (parental) and resistant to temsirolimus, were exposed to VPA, and tumor cell growth behavior compared. Temsirolimus resistance enhanced the number of tumor cells in the G2/M-phase, correlating with elevated cell proliferation and clonal growth. The cell cycling proteins cdk1 and cyclin B, along with Akt-mTOR signaling increased, whereas p19, p21 and p27 decreased, compared to the parental cells. VPA significantly reduced cell growth and up-regulated the acetylated histones H3 and H4. Cdk1 and cyclin B decreased, as did phosphorylated mTOR and the mTOR sub-complex Raptor. The mTOR sub-member Rictor and phosphorylated Akt increased under VPA. Knockdown of cdk1, cyclin B, or Raptor led to significant cell growth reduction. HDAC inhibition through VPA counteracts temsirolimus resistance, probably by down-regulating cdk1, cyclin B and Raptor. Enhanced Rictor and Akt, however, may represent an undesired feedback loop, which should be considered when designing future therapeutic regimens.
The Role of Platelets in Cancer Pathophysiology: Focus on Malignant Glioma
Authors: Marx Sascha , Xiao Yong , Baschin Marcel , Splittstöhser Maximilian , Altmann Robert , Moritz Eileen , Jedlitschky Gabriele , Bien-Möller Sandra , Schroeder Henry W.S. , Rauch Bernhard H.
Published: April 2019

The link between thrombocytosis and malignancy has been well known for many years and its associations with worse outcomes have been reported mainly for solid tumors. Besides measuring platelet count, it has become popular to assess platelet function in the context of malignant diseases during the last decade. Malignant gliomas differ tremendously from malignancies outside the central nervous system because they virtually never form distant metastases. This review summarizes the current understanding of the platelet–immune cell communication and its potential role in glioma resistance and progression. Particularly, we focus on platelet-derived proinflammatory modulators, such as sphingosine-1-phosphate (S1P). The multifaceted interaction with immune cells puts the platelet into an interesting perspective regarding the recent advances in immunotherapeutic approaches in malignant glioma.
Hydrogen Production from Formic Acid over Au Catalysts Supported on Carbon: Comparison with Au Catalysts Supported on SiO2 and Al2O3
Authors: Bulushev Dmitri A. , Sobolev Vladimir I. , Pirutko Larisa V. , Starostina Anna V. , Asanov Igor P. , Modin Evgenii , Chuvilin Andrey L. , Gupta Neeraj , Okotrub Alexander V. , Bulusheva Lyubov G.
Published: April 2019

Characteristics and catalytic activity in hydrogen production from formic acid of Au catalysts supported on porous N-free (Au/C) and N-doped carbon (Au/N-C) have been compared with those of Au/SiO2 and Au/Al2O3 catalysts. Among the catalysts examined, the Au/N-C catalyst showed the highest Au mass-based catalytic activity. The following trend was found at 448 K: Au/N-C > Au/SiO2 > Au/Al2O3, Au/C. The trend for the selectivity in hydrogen production was different: Au/C (99.5%) > Au/Al2O3 (98.0%) > Au/N-C (96.3%) > Au/SiO2 (83.0%). According to XPS data the Au was present in metallic state in all catalysts after the reaction. TEM analysis revealed that the use of the N-C support allowed obtaining highly dispersed Au nanoparticles with a mean size of about 2 nm, which was close to those for the Au catalysts on the oxide supports. However, it was by a factor of 5 smaller than that for the Au/C catalyst. The difference in dispersion could explain the difference in the catalytic activity for the carbon-based catalysts. Additionally, the high activity of the Au/N-C catalyst could be related to the presence of pyridinic type nitrogen on the N-doped carbon surface, which activates the formic acid molecule forming pyridinium formate species further interacting with Au. This was confirmed by density functional theory (DFT) calculations. The results of this study may assist the development of novel Au catalysts for different catalytic reactions.
Oncogenic Role of ZFAS1 lncRNA in Head and Neck Squamous Cell Carcinomas
Authors: Kolenda Tomasz , Guglas Kacper , Kopczyńska Magda , Teresiak Anna , Bliźniak Renata , Mackiewicz Andrzej , Lamperska Katarzyna , Mackiewicz Jacek
Published: April 2019

Background: Head and neck squamous cell carcinoma (HNSCC) is a heterogeneous disease with high mortality. The identification of specific HNSCC biomarkers will increase treatment efficacy and limit the toxicity of current therapeutic strategies. Long non-coding RNAs (lncRNAs) are promising biomarkers. Accordingly, here we investigate the biological role of ZFAS1 and its potential as a biomarker in HNSCC. Methods: The expression level of ZFAS1 in HNSCC cell lines was analyzed using qRT-PCR. Based on the HNSCC TCGA data, the ZFAS1 expression profile, clinicopathological features, and expression of correlated genes were analyzed in patient tissue samples. The selected genes were classified according to their biological function using the PANTHER tool. The interaction between lncRNA:miRNA and miRNA:mRNA was tested using available online tools. All statistical analyses were accomplished using GraphPad Prism 5. Results: The expression of ZFAS1 was up-regulated in the metastatic FaDu cell line relative to the less aggressive SCC-25 and SCC-040 and dysplastic DOK cell lines. The TCGA data indicated an up-regulation of ZFAS1 in HNSCCs compared to normal tissue samples. The ZFAS1 levels typically differed depending on the cancer stage and T-stage. Patients with a lower expression of ZFAS1 presented a slightly longer disease-free survival and overall survival. The analysis of genes associated with ZFAS1, as well its targets, indicate that they are linked with crucial cellular processes. In the group of patients with low expression of ZFAS1, we detected the up-regulation of suppressors and down-regulation of genes associated with epithelial-to-mesenchymal transition (EMT) process, metastases, and cancer-initiating cells. Moreover, the negative correlation between ZFAS1 and its host gene, ZNFX1, was observed. The analysis of interactions indicated that ZFAS1 has a binding sequence for miR-150-5p. The expression of ZFAS1 and miR-150-5p is negatively correlated in HNSCC patients. miR-150-5p can regulate the 3′UTR of EIF4E mRNA. In the group of patients with high expression of ZFAS1 and low expression of miR-150-5p, we detected an up-regulation of EIF4E. Conclusions: In HNSCC, ZFAS1 displays oncogenic properties, regulates important processes associated with EMT, cancer-initiating cells, and metastases, and might affect patients’ clinical outcomes. ZFAS1 likely regulates the cell phenotype through miR-150-5p and its downstream targets. Following further validation, ZFAS1 might prove a new and valuable biomarker.
Characterization of Meteorological Droughts Occurrences in Côte d’Ivoire: Case of the Sassandra Watershed
Authors: Santé Natacha , N’Go Yao Alexis , Soro Gneneyougo Emile , Meledje N’Diaye Hermann , Goula Bi Tié Albert
Published: April 2019

The Sassandra Basin, like most regions of Côte d’Ivoire, is increasingly affected by droughts that involve many environmental, social and economic impacts. This basin is full of several amenities such as hydroelectric dams, hydraulic and agricultural dams. There is also a strong agricultural activity. In the context of climate change, it is essential to analyze the occurrence of droughts in order to propose mitigation or adaptation measures for water management. The methodological approach consisted initially in characterizing the dry sequences by the use of the SPI (Standardized Precipitation Index) and secondly in determining the probabilities of occurrence of successive dry years using by Markov chains 1 and 2. The results indicate that most remarkable droughts in terms of intensity and duration occurred after the 1970s. A comparison of Markov matrices 1 and 2 between the period considered 1953–2015 with the periods 1953–1970 and 1971–2015 shows a profound change in the distribution of droughts at the different station. Thus, the probability of having two successive dry years is greater over the period 1970–2015 and is accentuated to the Southern and Northern regions (probabilities ranging from 71% to 80%) of the basin. Over the 1970–2015 period, the probability of obtaining three successive dry years is significantly high in this watershed (between 20% and 70%).
Feasibility and Surface Evaluation of the Pigment from Scytalidium cuboideum for Inkjet Printing on Textiles
Authors: Vega Gutierrez Sarath M. , He Yujuan , Cao Yu , Stone Derek , Walsh Zielle , Malhotra Rajiv , Chen Hsiou-Lien , Chang Chih-Hung , Robinson Seri C.
Published: April 2019

Textile inkjet printing is an increasingly popular process in the textile industry, as it allows for the incorporation of complex and detailed patterns onto fabrics, as well as the production of small and medium volumes of printed text. Unfortunately, most of the dyes used by the textile industry come from synthetic and/or non-renewable sources. There has been some research to date in using fungal pigments from wood rotting fungi (‘spalting’ fungi) as textile dyes, however these have never been tested in inkjet printing. Of particular interest is the red crystallizing pigment from Scytalidium cuboideum, which has previously shown exceptional stability on textiles. To test this pigment in an inkjet setting, cotton and polyester fabrics were printed with three different ink formulations involving the red pigment: hexadecyltrimethylammonium bromide (CTAB), ethanol, and acetone. The CTAB and ethanol-based ink formulations formed a ‘mesh-like’ structure on the surface of the cotton and polyester fibers, and turned the fabric purple. Acetone formulas formed crystal structures on the surface and turned the fabric red. These results show promise for turning the red pigment of S. cuboideum into an environmentally friendly, inkjet colorant, however further research is required to evaluate the crocking and explain the crystallization differences between inks.
Coating Effects of ε-Polylysine and Rosmarinic Acid Combined with Chitosan on the Storage Quality of Fresh Half-Smooth Tongue Sole (Cynoglossus semilaevis Günther) Fillets
Authors: Li Na , Liu Wenru , Shen Yong , Mei Jun , Xie Jing
Published: April 2019

The study was to evaluate the effects of chitosan-based coating combined with rosmarinic acid (RA) with different concentrations of ε-polylysine (ε-PL) on flavor retention and sensorial properties of half-smooth tongue sole fillets during storage at 4 °C. Results showed that chitosan-based coatings combined with ε-PL and RA contributed to the reduction of off-flavor compounds, such as total volatile base nitrogen (TVB-N), trimethylamine (TMA), and ATP-related compounds, and accumulation of free amino acids (FAAs). Nineteen volatile organic compounds were analyzed by gas chromatography-mass spectrometer (GC/MS) during storage, including seven alcohols, six aldehydes, and six ketones. The coating treated fresh half-smooth tongue sole (HTS) fillets significantly reduced the relative content of off-odor volatiles, such as 1-octen-3-ol, propanal, hexanal, and octanal. According to sensory evaluation results, chitosan-based coating combined with ε-PL and RA was an effective way to maintain quality of HTS fillets during refrigerated storage.
Direct Cryo Writing of Aerogels via 3D Printing of Aligned Cellulose Nanocrystals Inspired by the Plant Cell Wall
Authors: Kam Doron , Chasnitsky Michael , Nowogrodski Chen , Braslavsky Ido , Abitbol Tiffany , Magdassi Shlomo , Shoseyov Oded
Published: April 2019

Aerogel objects inspired by plant cell wall components and structures were fabricated using extrusion-based 3D printing at cryogenic temperatures. The printing process combines 3D printing with the alignment of rod-shaped nanoparticles through the freeze-casting of aqueous inks. We have named this method direct cryo writing (DCW) as it encompasses in a single processing step traditional directional freeze casting and the spatial fidelity of 3D printing. DCW is demonstrated with inks that are composed of an aqueous mixture of cellulose nanocrystals (CNCs) and xyloglucan (XG), which are the major building blocks of plant cell walls. Rapid fixation of the inks is achieved through tailored rheological properties and controlled directional freezing. Morphological evaluation revealed the role of ice crystal growth in the alignment of CNCs and XG. The structure of the aerogels changed from organized and tubular to disordered and flakey pores with an increase in XG content. The internal structure of the printed objects mimics the structure of various wood species and can therefore be used to create wood-like structures via additive manufacturing technologies using only renewable wood-based materials.
Ion Transport Behavior through Thermally Reduced Graphene Oxide Membrane for Precise Ion Separation
Authors: Hu Peizhuo , Huang Bochen , Miao Quanduo , Wang Haijing , Liu Lian , Tai Wenya , Liu Tonghuan , Li Zhan , Chen Suwen , Qian Lijuan
Published: April 2019

The cation transport behavior of thermally treated reduced graphene oxide membranes (GOMs) is reported. The GOMs were reduced by heat treatment at 25, 80, and 120 °C and then characterized by Fourier transform infrared spectroscopy, X-ray powder diffraction, and X-ray photoelectron spectroscopy to determine oxygen group content, C/O ratio, and layer spacing. The permeation rates of various cations with different sizes and charge numbers through these membranes were measured to understand the effect of the cations on transport behavior. The results indicated that the cation transport through the membranes depended on the layer spacing of the membrane and ion size and charge. Cations of the same valence permeating through the same GOM could be differentiated by their hydration radius, whereas the same type of cation passing through different GOMs could be determined by the spacing of the GOM layers. The cation valence strongly affected permeation behavior. The GOM that was prepared at 120 °C exhibited a narrow layer spacing and high separation factors for Mg/Ca, Mg/Sr, K/Ca, and K/Fe. The cations moving through the membrane could insert into the membrane lamellas, which neutralized the negative charge of the membrane, enlarged the layer spacing of the GOMs, and affected cation permeation.
Direct Amplification of High Energy Pulsed Laser in Fiber-Single Crystal Fiber with High Average Power
Authors: Li Feng , Yang Zhi , Lv Zhiguo , Yang Yang , Wang Yishan , Yang Xiaojun , Zhao Wei , Li Qianglong , Wei Yufeng
Published: April 2019

A laser master oscillator power amplifier (MOPA) system consisting of a fiber amplifier and a two-stage Yb:YAG single crystal fiber (SCF) is experimentally studied. The nonlinear stimulated Raman scattering (SRS) is avoided by limiting the output power of the fiber preamplifier to 600 mW. Due to the benefit from the low nonlinearity and high amplification gain of the SCF, a laser pulse duration of 16.95 ps and a high average power of 41.7 W at a repetition rate of 250 kHz are obtained by using a two-stage polarization controlled double-pass amplification of Yb:YAG SCF, corresponding to an output energy of 166.8 μJ and a peak power of 9.84 MW, respectively. The polarization controlled SCF amplification scheme achieved a gain as high as more than 69 times. During the amplification, the spectra gain narrowing effect and the polarization controlled four-pass amplification setup are also studied. The laser spectrum is narrowed from over 10 nm to less than 3 nm, and the pulse width is also compressed to hundreds of femtosecond by dechirping the laser pulse. This compact-sized, cost-effective laser source can be used in laser micromachining, or as the seeder source for generating much higher power and energy laser for scientific research. For some applications which need femtosecond laser, this laser source can also be compressed to femtosecond regime.
Numerical Simulation of Thermal-Solutal Capillary-Buoyancy Flow of Ge1–xSix Single Crystals Driven by Surface-Tension and Rotation in a Czochralski Configuration
Authors: Yu Jia-Jia , Zhang Lu , Shen Ting , Zhang Li , Li You-Rong
Published: April 2019

A series of three-dimensional numerical simulations were performed to understand the thermal-solutal capillary-buoyancy flow of Ge1-xSix melts during Czochralski crystal growth with a rotating crystal or crucible. The crystal and crucible rotation Reynolds numbers in this work are 0∼3.5 × 103 (0∼4.4 rpm) and 0∼−2.4 × 103 (0∼−1.5 rpm), respectively. Simulation results show that if the thermal capillary Reynolds number is relatively low, the flow will be steady and axisymmetric, even though the crystal or crucible rotates at a constant rate. The critical thermal capillary Reynolds number for the initiation of the three-dimensional oscillatory flow is larger than that of pure fluids. As the crystal or crucible rotation rate increases, the critical thermal capillary Reynolds number first increases and then decreases. The dominant flow pattern after the flow destabilization is azimuthal traveling waves. Furthermore, a reversed evolution from the oscillatory spoke pattern to traveling waves appears in the melt. Once the crystal or crucible rotation rate is relatively large, the traveling waves respectively evolve to rotating waves at the crystal rotation and a spindle-like pattern at the crucible rotation. In addition, the maximum amplitude of solute concentration oscillation on the free surface initially decreases, but finally rises with the crystal or crucible rotation rate increasing.
Unstable Limit Cycles and Singular Attractors in a Two-Dimensional Memristor-Based Dynamic System
Authors: Chang Hui , Song Qinghai , Li Yuxia , Wang Zhen , Chen Guanrong
Published: April 2019

This paper reports the finding of unstable limit cycles and singular attractors in a two-dimensional dynamical system consisting of an inductor and a bistable bi-local active memristor. Inspired by the idea of nested intervals theorem, a new programmable scheme for finding unstable limit cycles is proposed, and its feasibility is verified by numerical simulations. The unstable limit cycles and their evolution laws in the memristor-based dynamic system are found from two subcritical Hopf bifurcation domains, which are subdomains of twin local activity domains of the memristor. Coexisting singular attractors are discovered in the twin local activity domains, apart from the two corresponding subcritical Hopf bifurcation domains. Of particular interest is the coexistence of a singular attractor and a period-2 or period-3 attractor, observed in numerical simulations.
Entropy Rate Superpixel Classification for Automatic Red Lesion Detection in Fundus Images
Authors: Romero-Oraá Roberto , Jiménez-García Jorge , García María , López-Gálvez María I. , Oraá-Pérez Javier , Hornero Roberto
Published: April 2019

Diabetic retinopathy (DR) is the main cause of blindness in the working-age population in developed countries. Digital color fundus images can be analyzed to detect lesions for large-scale screening. Thereby, automated systems can be helpful in the diagnosis of this disease. The aim of this study was to develop a method to automatically detect red lesions (RLs) in retinal images, including hemorrhages and microaneurysms. These signs are the earliest indicators of DR. Firstly, we performed a novel preprocessing stage to normalize the inter-image and intra-image appearance and enhance the retinal structures. Secondly, the Entropy Rate Superpixel method was used to segment the potential RL candidates. Then, we reduced superpixel candidates by combining inaccurately fragmented regions within structures. Finally, we classified the superpixels using a multilayer perceptron neural network. The used database contained 564 fundus images. The DB was randomly divided into a training set and a test set. Results on the test set were measured using two different criteria. With a pixel-based criterion, we obtained a sensitivity of 81.43% and a positive predictive value of 86.59%. Using an image-based criterion, we reached 84.04% sensitivity, 85.00% specificity and 84.45% accuracy. The algorithm was also evaluated on the DiaretDB1 database. The proposed method could help specialists in the detection of RLs in diabetic patients.
Role of Quantum Entropy and Establishment of H-Theorems in the Presence of Graviton Sinks for Manifestly-Covariant Quantum Gravity
Authors: Tessarotto Massimo , Cremaschini Claudio
Published: April 2019

Based on the introduction of a suitable quantum functional, identified here with the Boltzmann–Shannon entropy, entropic properties of the quantum gravitational field are investigated in the framework of manifestly-covariant quantum gravity theory. In particular, focus is given to gravitational quantum states in a background de Sitter space-time, with the addition of possible quantum non-unitarity effects modeled in terms of an effective quantum graviton sink localized near the de Sitter event horizon. The theory of manifestly-covariant quantum gravity developed accordingly is shown to retain its emergent-gravity features, which are recovered when the generalized-Lagrangian-path formalism is adopted, yielding a stochastic trajectory-based representation of the quantum wave equation. This permits the analytic determination of the quantum probability density function associated with the quantum gravity state, represented in terms of a generally dynamically-evolving shifted Gaussian function. As an application, the study of the entropic properties of quantum gravity is developed and the conditions for the existence of a local H-theorem or, alternatively, of a constant H-theorem are established.
Plant Species Recognition Skills in Finnish Students and Teachers
Authors: Kaasinen Arja
Published: April 2019

Limited awareness about nature and its species can have a negative influence on children’s relationship to nature. Plant species recognition and outdoor education are perhaps the easiest way to approach nature relationships and increase knowledge. Unfortunately, it has been shown that people do not recognize plant species very well. This phenomenon is called “plant blindness”. This study presents information about the phenomenon in Finland. The purpose of this research was to determine how well Finnish students from different age groups recognize plant species and which variables explain recognition of plant species in general education in Finland. The subjects were pupils from primary school to university teachers. A total of 754 people took part in the research. The results showed that Finnish pupils do not recognize plant species very well, with wide variations in responses between student levels. Species recognition skills improved from primary school to university teachers.
A Capacitor Voltage Balancing Approach Based on Mapping Strategy for MMC Applications
Authors: Ricco Mattia , Mathe Laszlo , Hammami Manel , Franco Francesco Lo , Rossi Claudio , Teodorescu Remus
Published: April 2019

This paper proposes a new strategy to achieve balanced capacitor voltages in modular multilevel converters. Among the possible solutions, centralized arm control approaches are often adopted. These methods require a balancing technique based on a sorted list of the sub-modules according to their capacitor voltages. In order to achieve the aforementioned sorted list, different algorithms have been proposed in literature, such as: Sorting algorithms, max/min approaches, etc. However, the sorting algorithms require a long execution time, while the max/min approaches affect the converter dynamic response during faults. To overcome these issues, a new mapping strategy providing a quasi-sorted list is proposed in this paper. The suggested method is compared in simulation with both the classical bubble sorting algorithm, and the max/min method during both normal and faulty conditions. Moreover, the three methods have been implemented in a Xilinx Zynq-7000 System-on-Chip (SoC) device, in order to analyze the corresponding execution time and the required computational effort. Hardware-in-the-loop results are presented for demonstrating the superior performance of the proposed balancing strategy.
Multi-Channel Optoelectronic Measurement System for Soil Nutrients Analysis
Authors: Ma Liuzheng , Li Zhenfeng , Birech Zephania , Li Shixin , Yang Yatao , Zhang Wei , Hu Jiandong
Published: April 2019

To solve the problems that occur when farmers overuse chemical fertilizers, it is necessary to develop rapid and efficient portable measurement systems for the detection and quantification of nitrogen (N), phosphorus (P), and potassium (K) in soil. Challenges arise from the use of currently available portable instruments which only have a few channels, namely measurement and the reference channels. We report on a home-built, multichannel, optoelectronic measurement system with automatically switching light sources for the detection of N, P, K content in soil samples. This optoelectronic measurement system consists of joint LED light sources with peak emission wavelengths of 405 nm, 660 nm, and 515 nm, a photodiode array, a circuit board with a microcontroller unit (MCU), and a liquid-crystal display (LCD) touch screen. The straightforward principle for rapid detection of the extractable nutrients (N, P, K) was well-established, and characterization of the designed measurement system was done. Using this multi-channel measurement system, available nutrients extracted from six soil samples could be measured simultaneously. The absorbance compensation, concentration calibration, and nutrition measurements were performed automatically to achieve high consistency across six channels. The experimental results showed that the cumulative relative standard deviations of 1.22%, 1.27%, and 1.00% were obtained from six channels with known concentrations of standard solutions, respectively. The coefficients of correlation for the detection of extracted nutrients of N, P, K content in soil samples using both the proposed method and conventional lab-based method were 0.9010, 0.9471, and 0.8923, respectively. Experimental results show that this optoelectronic measurement system can perform the measurement of N, P, K contents of six soil samples simultaneously and may be used as an actual tool in determining nutrients in soil samples with an improvement in detection efficiency.
Preliminary Techno–Environment–Economic Evaluation of an Innovative Hybrid Renewable Energy Harvester System for Residential Application
Authors: Wang Xiaohang , Chong Wentong , Wong Kokhoe , Lai Saihin , Saw Liphuat , Xiang Xianbo , Wang Chin-Tsan
Published: April 2019

A technical, environmental, and economic feasibility study for a patented hybrid renewable energy harvester system for residential application is conducted in this paper. This system can be mounted on top of an existing residential building to provide electricity from renewable sources. The system is characterized by its V-shaped roof guide vane (VRGV) that directs and augments airflow into the wind turbine, to enhance the rotational and power generation performance of the wind turbines in low wind speed areas. Furthermore, the VRGV increases the installation area for the solar photovoltaic panels and expand the rainwater collection area for the building, and facilitates natural ventilation and prevents excessive solar radiation into the room. The environment–economic evaluation of the system is conducted based on the life-cycle cost (LCC) in terms of low carbon and economic cost-effectiveness. The evaluation of the system with dimensions of 15 m (L) × 16 m (W) × 17.05 m (H) showed that the annual energy generated is 21.130 MWh. Annual low-carbon benefit of the system is estimated to be 11.894 t. The cumulative net present value (NPV) of the system in the life cycle time (20 years) is $52,207.247, with the consideration of a discount rate of 8%; also, the cash flow breakeven occurs in the 11th year. It is important to note that the carbon payback period (CPP) of the system is five years.
Visual Experimental Study on Gradation Optimization of Two-Stage Gravel Packing Operation in Unconventional Reservoirs
Authors: Meng Xingbang , Qi Minhui , Meng Zhan , Li Tong , Niu Zhongxiao
Published: April 2019

During the development of unconventional reservoirs with high sand production rate and fine silt content such as heavy oil and hydrate reservoirs, silt sand blockage problem is a serious issue. A two-stage gravel-packing sand control technique is applied to solve the silt sand blockage now. However, traditional experiments on this technique could not obtain the dynamic distribution law of intrusive sand in the gravel pack. In this study, a new visualization experiment based on hydrodynamic similarity criterion for studying particle blockage in gravel packs was conducted. Real-time monitoring of sand particle migration in the gravel pack could be achieved. Also, the stable penetration depth and the distributing disciplinarian of invaded particles could be determined. The results show that when the gravel-to-sand median size ratio of gravel bed I is less than five, the sand bridge can be formed at the front end of the gravel pack. This could prevent sand from further intruding. As the grain size of gravel bed II is increased, the flow velocity is reduced. Thus, the sand invading into gravel bed II tends to settle at the interface. A large amount of sand intrusion can happen to gravel pack II when the pore filling front breaks through the gravel bed I.
Electric Field Effect on the Thermal Decomposition and Co-combustion of Straw with Solid Fuel Pellets
Authors: Barmina Inesa , Kolmickovs Antons , Valdmanis Raimonds , Zake Maija , Vostrikovs Sergejs , Kalis Harijs , Strautins Uldis
Published: April 2019

The aim of this study was to provide more effective use of straw for energy production by co-firing wheat straw pellets with solid fuels (wood, peat pellets) under additional electric control of the combustion characteristics at thermo-chemical conversion of fuel mixtures. Effects of the DC electric field on the main combustion characteristics were studied experimentally using a fixed-bed experimental setup with a heat output up to 4 kW. An axisymmetric electric field was applied to the flame base between the positively charged electrode and the grounded wall of the combustion chamber. The experimental study includes local measurements of the composition of the gasification gas, flame temperature, heat output, combustion efficiency and of the composition of the flue gas considering the variation of the bias voltage of the electrode. A mathematical model of the field-induced thermo-chemical conversion of combustible volatiles has been built using MATLAB. The results confirm that the electric field-induced processes of heat and mass transfer allow to control and improve the main combustion characteristics thus enhancing the fuel burnout and increasing the heat output from the device up to 14% and the produced heat per mass of burned solid fuel up to 7%.
The Possibility of Generating Electricity Using Small-Scale Wind Turbines and Solar Photovoltaic Systems for Households in Northern Cyprus: A Comparative Study
Authors: Kassem Youssef , Al Zoubi Raafat , Gökçekuş Hüseyin
Published: April 2019

The increased energy demand and related environmental problems caused by burning fossil fuels have raised interest in alternative energy sources. This study investigated the wind characteristics and available wind energy for three urban regions in Northern Cyprus using the Weibull distribution function. The results illustrate that Gazimağusa is the most applicable location for harvesting the kinetic energy of the wind compared to Lefkoşa and Girne. Moreover, the solar potential at a specific location can be analyzed using a different simulation tool. In the present paper, the performance of a rooftop Photovoltaic (PV) system for household buildings in three selected is assessed. Three types of simulation software (PVGIS, PV*SOL, and PVWatts) are used to evaluate the performance of the 6.4 kWp grid-connected rooftop PV system. This study assessed the energy generation, performance ratio and capacity factor for this PV system. The results concluded that PVGIS is an easy, fast, and reliable software tool that can be used for the simulation of a solar PV system in the studied regions. Furthermore, an economic evaluation of renewable systems in the three urban regions is presented. As a result, a small-scale grid-connected solar/wind system that is able to generate electricity with an excellent percentage of clean energy was proposed and developed. The analysis indicates that the proposed PV projects showed significant potential in the studied locations. In addition, the proposed PV system is the most economical option for generating electricity compared to wind systems due to the low electricity prices and of the ability to recover the initial investment. Consequently, it is expected that the simulation results will help in demonstrating the advantages and challenges of installing grid-connected PV systems for households in Northern Cyprus in order to reduce the electricity consumption produced by fossil fuels.
Effects of Phosphate Solubilizing Bacteria on the Growth, Photosynthesis, and Nutrient Uptake of Camellia oleifera Abel.
Authors: Wu Fei , Li Jianrong , Chen Yanliu , Zhang Linping , Zhang Yang , Wang Shu , Shi Xin , Li Lei , Liang Junsheng
Published: April 2019

Phosphorus (P) is a necessary nutrient for plant growth and plays an important role in plant metabolisms; however, the majority of P in soil is in insoluble forms. Phosphate solubilizing bacteria (PSB) can convert the insoluble phosphates into plant-available forms and may have the potential for use in sustainable agricultural practices. This study examined the effects of two native PSB, namely Bacillus aryabhattai (JX285) and Pseudomonas auricularis (HN038), and a mixture of both strains (1:1) on the growth of Camellia oleifera Abel. seedlings. The results showed a significant promotion of the growth of C. oleifera plants by three inoculation treatments. All the PSB inoculation treatments could improve the leaf nitrogen (N) and P content and had positive effects on the available N, P, and potassium (K) content of rhizosphere soil. A co-inoculation of the two native PSB strains caused a synergistic effect and achieved the best benefit. In conclusion, B. aryabhattai and P. auricularis could be used as biological agents instead of chemical fertilizers for agricultural production to reduce environmental pollution and increase the yield of tea oil.
Effects of Planting Density on Soil Bulk Density, pH and Nutrients of Unthinned Chinese Fir Mature Stands in South Subtropical Region of China
Authors: Duan Aiguo , Lei Jie , Hu Xiaoyan , Zhang Jianguo , Du Hailun , Zhang Xiongqing , Guo Wenfu , Sun Jianjun
Published: April 2019

Chinese fir (Cunninghamia lanceolata (Lamb.) Hook) is a fast-growing evergreen conifer with high-quality timber and is an important reforestation and commercial tree species in southern China. Planting density affects the productivity of Chinese fir plantations. To study the effect of five different planting densities and soil depth on soil nutrient contents of a mature C. lanceolata plantation, the soil nutrient contents (soil depths 0–100 cm) of 36-year-old mature Chinese fir plantations under five different planting densities denoted A (1667 trees·ha−1), B (3333 trees·ha−1), C (5000 trees·ha−1), D (6667 trees·ha−1), and E (10,000 trees·ha−1) were measured in Pingxiang county, Guangxi province, China. Samples were collected from the soil surface down to a one meter depth from each of 45 soil profiles, and soil samples were obtained at 10 different soil depths of 0–10, 10–20, 20–30, 30–40, 40–50, 50–60, 60–70, 70–80, 80–90, and 90–100 cm. Twelve soil physical and chemical indicators were analyzed. The results showed that: (1) as planting density increased, the organic matter, organic carbon, total N and P, available N, effective Fe, and bulk density decreased. Soil pH, total K, and effective K increased with increasing planting density. Planting density did not significantly influence the exchangeable Ca and Mg. (2) Soil organic matter; organic carbon; total N and P; effective N, P, and K; exchangeable Ca and Mg; effective Fe content; and bulk density decreased with increasing soil depth. This pattern was particularly evident in the top 30 cm of the soil. (3) Excessively high planting density is not beneficial to the long-term maintenance of soil fertility in Chinese fir plantations, and the planting density of Chinese fir plantations should be maintained below 3333 stems·ha−1 (density A or B) to maintain soil fertility while ensuring high yields.
A Relaxation Filtering Approach for Two-Dimensional Rayleigh–Taylor Instability-Induced Flows
Authors: Rahman Sk. Mashfiqur , San Omer
Published: April 2019

In this paper, we investigate the performance of a relaxation filtering approach for the Euler turbulence using a central seven-point stencil reconstruction scheme. High-resolution numerical experiments are performed for both multi-mode and single-mode inviscid Rayleigh–Taylor instability (RTI) problems in two-dimensional canonical settings. In our numerical assessments, we focus on the computational performance considering both time evolution of the flow field and its spectral resolution up to three decades of inertial range. Our assessments also include an implicit large eddy simulation (ILES) approach that is based on a fifth-order weighted essential non-oscillatory (WENO) with built-in numerical dissipation due to its upwind-based reconstruction architecture. We show that the relaxation filtering approach equipped with a central seven-point stencil, sixth-order accurate discrete filter yields accurate results efficiently, since there is no additional cost associated with the computation of the smoothness indicators and interface Riemann solvers. Our a-posteriori spectral analysis also demonstrates that its resolution capacity is sufficiently high to capture the details of the flow behavior induced by the instability. Furthermore, its resolution capability can be effectively controlled by the filter shape and strength.
Effects of Two Different Irrigation Systems on the Amino Acid Concentrations, Volatile Composition and Sensory Profiles of Godello Musts and Wines
Authors: Mirás-Avalos José Manuel , Bouzas-Cid Yolanda , Trigo-Córdoba Emiliano , Orriols Ignacio , Falqué Elena
Published: April 2019

The concentrations of amino acids and volatile compounds of a given grapevine cultivar may be modified by climate variability between years and by management practices, such as irrigation, that may alter the typicality of its wines. The current study aimed at assessing the amino acid profile of musts and wines, volatile composition and sensory profile of wines from Vitis vinifera (L.) cultivar Godello under rain-fed and two drip irrigation systems (above, drip irrigation (DI), and under the soil surface, subsurface drip irrigation (SDI)) over three consecutive years. Irrigation tended to increase must and wine total acidity; however, it did not alter must amino acid concentrations significantly. Irrigation reduced the concentrations of acetaldehyde and methanol in Godello wines. Moreover, irrigation tended to decrease the concentrations of compounds giving fruity aromas, such as acetaldehyde (by 31% in SDI) and isoamyl acetate (by 21% in SDI), when compared to rain-fed conditions. Sensory analysis revealed slight differences between treatments. Rain-fed and SDI were the treatments showing the greatest differences. Weather conditions affected more must and wine composition than in-season effects caused by irrigation.
Quality Characteristics Analysis and Remaining Shelf Life Prediction of Fresh Tibetan Tricholoma matsutake under Modified Atmosphere Packaging in Cold Chain
Authors: Fu Zetian , Zhao Shuang , Zhang Xiaoshuan , Polovka Martin , Wang Xiang
Published: April 2019

Tricholoma matsutake (T. matsutake) growing in Tibet is very popular for its high economic and medicinal value, but fresh T. matsutake has an extremely short shelf life. The shelf life of T. matsutake is complex, influenced by product characteristics, surrounding environmental conditions, and spoilage development. The objective of this work was to study the quality characteristics of fresh T. matsutake during its shelf life period in modified atmosphere packaging (MAP) conditions and establish its remaining shelf life prediction models in a cold chain. In this study, we measured and analyzed quality indicators of fresh T. matsutake, including hardness (cap, stipe), color, odor of sensory characteristics, pH, soluble solids content (SSC), and moisture content (MC) of physical and chemical characteristics under the temperature condition of 4 °C and relative humidity (RH) of 90%. The sensory evaluation results showed that the odor indicator in sensory characteristics was more sensitive to the freshness of T. matsutake. The changes of pH, SSC, and MC were divided into three periods to analyze the physiological changes of T. matsutake. The cap spread process could affect the changes of pH, SSC, and MC in period S1, and they changed gradually in period S2. In the period S3, they changed complicatedly because of deterioration. The remaining shelf life prediction model of T. matsutake was established by the back propagation (BP) neural network method to quantify the relationship between the quality indicators and the remaining shelf life. The shelf life characteristics are complex, which were optimized by correlation analysis. Significant benefits of this work are anticipated on the transportation and preservation of fresh T. matsutake to the market and the reduction of its losses in the postharvest chain.
Influence of Supplemental Feed Choice for Pasture-Based Cows on the Fatty Acid and Volatile Profile of Milk
Authors: O’Callaghan Tom F. , Mannion David , Apopei Diana , McCarthy Noel A. , Hogan Sean A. , Kilcawley Kieran N. , Egan Michael
Published: April 2019

The purpose of this study was to examine the impact of a variety of supplemental feeds on the composition and quality of milk in a pasture-based dairy system. Four pasture-supplemented feeding systems were compared: Group 1 supplementation with 16% crude protein parlour concentrate (CONC); Group 2 supplementation with palm kernel expeller plus parlour concentrate (PKE); Group 3 supplemented with soya hulls plus parlour concentrate (SOYA); Group 4 was supplemented with molassed beet pulp plus parlour concentrate (BEET). Supplemental feeding system was demonstrated to have a significant effect on the size of native casein micelles and the gelation properties of milks. While CONC feeding produced significantly higher casein micelle size, gel strength (Young’s Modulus) was significantly negatively correlated with casein micelle size. Supplemental feeding system had a significant effect on a number of fatty acids (FA) and indices derived therefrom, including total saturated and unsaturated fatty acids, de novo produced FA, omega 3, and omega 6 FA. The volatile profile of milks was also affected by supplemental feed choice, whereby multivariate analysis demonstrated that the CONC diet was distinctly different to that of the PALM, SOYA, and BEET milks. Multivariate analysis demonstrated that it is possible to distinguish milks from different pasture-supplemented feeding systems by their FA profile.
Mechanical Properties of Ca-Saturated Hydrogels with Functionalized Alginate
Authors: Dalheim Marianne Ø. , Omtvedt Line Aa. , Bjørge Isabel M. , Akbarzadeh Anita , Mano João F. , Aachmann Finn L. , Strand Berit L.
Published: April 2019

In this work, the mechanical properties and stability of alginate hydrogels containing functionalized alginates (peptide and β-cyclodextrin) were studied. There is an increasing interest in the modification of alginates to add functions such as cell attachment and increased solubility of hydrophobic drugs, for better performance in tissue engineering and drug release, respectively. Functionalization was achieved in this study via periodate oxidation followed by reductive amination, previously shown to give a high and controllable degree of substitution. Young’s modulus and the stress at rupture of the hydrogels were in general lowered when exchanging native alginate with the modified alginate. Still, the gel strength could be adjusted by the fraction of modified alginate in the mixed hydrogels as well as the degree of oxidation. No notable difference in deformation at rupture was observed while syneresis was influenced by the degree of oxidation and possibly by the nature and amount of the grafted molecules. The mixed hydrogels were less stable than hydrogels with only native alginate, and modified alginate was released from the hydrogels. Furthermore, the hydrogels in general rather disintegrated than swelled upon saline treatments.
A Novel Aquaporin 12-like Protein from Chilo suppressalis: Characterization and Functional Analysis
Authors: Lu Ming-Xing , Song Jie , Xu Jing , Wang Guirong , Liu Yang , Du Yu-Zhou
Published: April 2019

Aquaporins (AQPs), which are members of the major intrinsic protein (MIP) family, play an important role in the transport of water and other small, uncharged solutes across membranes. In this study, we identified gene encoding two aquaporin 12-like (AQP12L) proteins, CsAqp12L_v1 and CsAqp12L_v2, from Chilo suppressalis, a serious rice pest in Asia. Phylogenetic analysis indicated that CsAQP12L_V1 and CsAQP12L_V2 were grouped in a well-supported cluster that included other members of Lepidoptera. The two proteins are almost identical, except that CsAQP12L_V1 lacks 34 amino acids that are present in CsAQP12L_V2 at site 217. The qRT-PCR indicated that both CsAqp12L and CsAqp12L_v2 were expressed in heads, epidermis, foregut, midgut, and hindguts, with the highest level of expression in hindguts, heads, and epidermis. Expression of CsAqp12L and CsAqp12L_v2 was detected in all life stages and both sexes and was highest in first instar larvae and lowest in eggs. Expression of CsAqp12L and CsAqp12L_v2 was not significantly altered by exposure to brief changes in temperature. There were no significant differences in the third instar larvae, male and female pupae, and female adults in response to adverse humidity. However, the mRNA level of CsAqp12L in the fifth instar larvae and CsAqp12L_v2 in male adults was induced significantly by low humidity, respectively. Moreover, Xenopus oocytes injected with cRNAs of CsAQP12L_V1 and CsAQP12L_V2 showed no significant changes in permeability to water, glycerol, trehalose, or urea. The two CsAQP12L variants likely localize to an intracellular location in C. suppressalis and may respond to novel stimuli.