bluuebunny/crossref_metadata_2025 huggingface dataset:
Poultry and Wild Birds as a Reservoir of CMY-2 Producing Escherichia coli: The First Large-Scale Study in Greece
Authors: Athanasakopoulou Zoi , Tsilipounidaki Katerina , Sofia Marina , Chatzopoulos Dimitris C. , Giannakopoulos Alexios , Karakousis Ioannis , Giannakis Vassilios , Spyrou Vassiliki , Touloudi Antonia , Satra Maria , Galamatis Dimitrios , Diamantopoulos Vassilis , Mpellou Spyridoula , Petinaki Efthymia , Billinis Charalambos
Published: February 2021

Resistance mediated by β-lactamases is a globally spread menace. The aim of the present study was to determine the occurrence of Escherichia coli producing plasmid-encoded AmpC β-lactamases (pAmpC) in animals. Fecal samples from chickens (n = 159), cattle (n = 104), pigs (n = 214), and various wild bird species (n = 168), collected from different Greek regions during 2018–2020, were screened for the presence of pAmpC-encoding genes. Thirteen E. coli displaying resistance to third-generation cephalosporins and a positive AmpC confirmation test were detected. blaCMY-2 was the sole pAmpC gene identified in 12 chickens’ and 1 wild bird (Eurasian magpie) isolates and was in all cases linked to an upstream ISEcp1-like element. The isolates were classified into five different sequence types: ST131, ST117, ST155, ST429, and ST1415. Four chickens’ stains were assigned to ST131, while five chickens’ strains and the one from the Eurasian magpie belonged to ST117. Seven pAmpC isolates co-harbored genes conferring resistance to tetracyclines (tetM, tetB, tetC, tetD), 3 carried sulfonamide resistance genes (sulI and sulII), and 10 displayed mutations in the quinolone resistance-determining regions of gyrA (S83L+D87N) and parC (S80I+E84V). This report provides evidence of pAmpC dissemination, describing for the first time the presence of CMY-2 in chickens and wild birds from Greece.
Consideration of Sex as a Biological Variable in the Development of Doxorubicin Myotoxicity and the Efficacy of Exercise as a Therapeutic Intervention
Authors: Montalvo Ryan N. , Doerr Vivian , Nguyen Branden L. , Kelley Rachel C. , Smuder Ashley J.
Published: February 2021

Doxorubicin (DOX) is an anthracycline antibiotic used to treat a wide variety of hematological and solid tumor cancers. While DOX is highly effective at reducing tumor burden, its clinical use is limited by the development of adverse effects to both cardiac and skeletal muscle. The detrimental effects of DOX to muscle tissue are associated with the increased incidence of heart failure, dyspnea, exercise intolerance, and reduced quality of life, which have been reported in both patients actively receiving chemotherapy and cancer survivors. A variety of factors elevate the probability of DOX-related morbidity in patients; however, the role of sex as a biological variable to calculate patient risk remains unclear. Uncertainty regarding sexual dimorphism in the presentation of DOX myotoxicity stems from inadequate study design to address this issue. Currently, the majority of clinical data on DOX myotoxicity come from studies where the ratio of males to females is unbalanced, one sex is omitted, and/or the patient cohort include a broad age range. Furthermore, lack of consensus on standard outcome measures, difficulties in long-term evaluation of patient outcomes, and other confounding factors (i.e., cancer type, drug combinations, adjuvant therapies, etc.) preclude a definitive answer as to whether differences exist in the incidence of DOX myotoxicity between sexes. This review summarizes the current clinical and preclinical literature relevant to sex differences in the incidence and severity of DOX myotoxicity, the proposed mechanisms for DOX sexual dimorphism, and the potential for exercise training to serve as an effective therapeutic countermeasure to preserve muscle strength and function in males and females.
Effect of Antioxidant Therapy on Oxidative Stress in Vivo
Authors: Pasini Anna Maria Fratta , Cominacini Luciano
Published: February 2021

Over the last few decades, many efforts have been put into fields that explore the potential benefits of antioxidants, especially with regards to aging, cancer, cardiovascular diseases, and neurodegenerative diseases [...]
Hole Concealment Algorithm Using Camera Parameters in Stereo 360 Virtual Reality System
Authors: Cha Sangguk , Nam Da-Yoon , Han Jong-Ki
Published: February 2021

Virtual reality (VR) has been one of the most important topics in the field of multimedia signals and systems for approximately 10 years [...]
Identifying and Evaluating Vocation-Related Neuro-Musculoskeletal Deficiencies in Professional Musicians: A Review
Authors: Mizrahi Joseph
Published: February 2021

A combination of factors exposes musicians to neuro-musculoskeletal disorders, which lead to pain and damage. These involve overuse due to long playing hours, containing repetitive movements under stressful conditions, usually performed in an unnatural posture. Although the evoked disorders are usually non-traumatic, they may often lead to prolonged or even permanent damage. For instance, in upper string players, these include bursitis and tendinopathies of the shoulder muscles, tendonitis of the rotator cuff, injury at the tendon sheaths, medial or lateral epicondylitis (also known as tennis elbow), myofascial pain, and wrist tendonitis (also known as carpal tunnel syndrome, or De Quervein’s syndrome). In cases of intensive performance, a traumatic injury may result, requiring drastic means of intervention such as surgery. It should be pointed out that the upper body and upper extremities are the most commonly affected sites of playing musicians. This review provides a description of the playing-related motor disorders in performing musicians, and of the methodologies used to identify and evaluate these disorders, particularly for violinists and other upper string players.
Evaluation of Organ Dose and Image Quality Metrics of Pediatric CT Chest-Abdomen-Pelvis (CAP) Examination: An Anthropomorphic Phantom Study
Authors: Muhammad Nor Azura , Kayun Zunaide , Abu Hassan Hasyma , Wong Jeannie Hsiu Ding , Ng Kwan Hoong , Karim Muhammad Khalis Abdul
Published: February 2021

The aim of this study is to investigate the impact of CT acquisition parameter setting on organ dose and its influence on image quality metrics in pediatric phantom during CT examination. The study was performed on 64-slice multidetector CT scanner (MDCT) Siemens Definition AS (Siemens Sector Healthcare, Forchheim, Germany) using various CT CAP protocols (P1–P9). Tube potential for P1, P2, and P3 protocols were fixed at 100 kVp while P4, P5, and P6 were fixed at 80 kVp with used of various reference noise values. P7, P8, and P9 were the modification of P1 with changes on slice collimation, pitch factor, and tube current modulation (TCM), respectively. TLD-100 chips were inserted into the phantom slab number 7, 9, 10, 12, 13, and 14 to represent thyroid, lung, liver, stomach, gonads, and skin, respectively. The image quality metrics, signal to noise ratio (SNR) and contrast to noise ratio (CNR) values were obtained from the CT console. As a result, this study indicates a potential reduction in the absorbed dose up to 20% to 50% along with reducing tube voltage, tube current, and increasing the slice collimation. There is no significant difference (p > 0.05) observed between the protocols and image metrics.
Design of a Modular Plantar Orthosis System through the Application of TRIZ Methodology Tools
Authors: Ramírez-Rios Lidia Yolanda , Camargo-Wilson Claudia , Olguín-Tiznado Jesús Everardo , López-Barreras Juan Andrés , Inzunza-González Everardo , García-Alcaraz Jorge Luis
Published: February 2021

Innovations within the medical device sector are constantly and rapidly emerging due to increasing demand, especially for orthosis systems, which usually constitute built rigids with low comfort, due mainly to the standardized production process. This article reports the design process of a plantar orthosis system from the application of Theory of Inventive Problem Solving (TRIZ) tools, known as generic parameters, matrix of contradictions, and inventive principles. The final orthosis is integrated by four modules or components (1 central and 3 movables) and customers can buy only the required ones, reducing cost in unnecessary parts. The plantar orthosis was defined based on three engineering parameters that delimited the design work by developing a customizable system that is capable of performing simultaneous functions and whose manufacture could be standardized. We identified the existence of a technical contradiction between the engineering parameters, customization, and standardization, which was solved by the inventive principles of segmentation, inversion, transition to a new dimension, and porous materials. A modular design with four components was accomplished, molds are built for each component in a machining center and injected using granulated ethylene vinyl acetate copolymer. The positions of the movable components are configured through a bolt-hole assembly mechanism to the central component, which is a flat perforated plantar base. The novelty in the design here presented is elated to supports that constitute the orthosis, which are interchangeable and adjustable to the pathological and morphological needs of each patient.
Prediction of the Mechanical Performance of High-Strength Concrete Containing Biomedical Polymeric Waste Obtained from Dialysis Treatment
Authors: Rahimireskati Saman , Ghabraie Kazem , Garcez Estela Oliari , Al-Ameri Riyadh
Published: February 2021

Since between 1.5 and 8 kg (400 kg/patient/year) of biomedical polymeric waste (BPW) is usually discarded by landfilling or combusting after each dialysis treatment, this study provides evidence for safe and environment-friendly utilisation of BPW, sourced from dialysis treatment and donated by the health and industrial partners, by incorporating it in high-strength concrete. Moreover, the paper aims to provide engineers, designers, and the construction industry with information regarding the mechanical performance of high-strength concrete containing BPW, and the susceptibility of the current international codes and standards on the prediction of the mechanical performance. A new concrete mix design incorporating BPW was proposed and verified by several trial mixes. Three Soft, Hard, and Hybrid BPW were added to the conventional high-strength concrete in different percentages ranging from 1.5% to 9% by weight of cement. Afterwards, the fresh and hardened concrete properties, namely slump, density, compressive strength, tensile strength, modulus of elasticity, and Scanning Electron Microscopy (SEM), were investigated, and existing prediction models were employed to verify their suitability for the new concrete. Generally, adding Hybrid BPW resulted in better mechanical performance than soft or hard BPW addition, while eliminating the waste separation phase. The results also showed that the mechanical performance of BPW-containing concrete is predictable by current codes, addressing possible engineering design limitations. New higher accuracy regression-based models were also proposed to reach better engineering interpretations.
Lightweight Unmanned Aerial System for Time-Domain Electromagnetic Prospecting—The Next Stage in Applied UAV-Geophysics
Authors: Parshin Alexander , Bashkeev Ayur , Davidenko Yuriy , Persova Marina , Iakovlev Sergey , Bukhalov Sergey , Grebenkin Nikolay , Tokareva Marina
Published: February 2021

Nowadays in solving geological problems, the technologies of UAV-geophysics, primarily magnetic and gamma surveys, are being increasingly used. However, for the formation of the classical triad of airborne geophysics methods in the UAV version, there was not enough technology for UAV-electromagnetic sounding, which would allow studying the geological environment at depths of tens and hundreds of meters with high detail. This article describes apparently the first technology of UAV-electromagnetic sounding in the time domain (TDEM, TEM), implemented as an unmanned system based on a light multi-rotor UAV. A measuring system with an inductive sensor—an analogue of a 20 × 20 or 50 × 50 m receiving loop is towed by a UAV, and a galvanically grounded power transmitter is on the ground and connected to a pulse generator. The survey is carried out along a network of parallel lines at low altitude with a terrain draping at a speed of 7–8 m/s, the maximum distance of the UAV’s departure from the transmitter line can reach several kilometers, thus the created technology is optimal for performing detailed areal electromagnetic soundings in areas of several square kilometers. The results of the use of the unmanned system (UAS) in real conditions of the mountainous regions of Eastern Siberia are presented. Based on the obtained data, the sensitivity of the system was simulated and it was shown that the developed technology allows one to collect informative data and create geophysical sections and maps of electrical resistivity in various geological situations. According to the authors, the emergence of UAV-TEM systems in the near future will significantly affect the practice of geophysical work, as it was earlier with UAV-magnetic prospecting and gamma-ray survey.
Innovative Approaches for Drinking- and Waste-Water Treatment: An Editorial Review Summarizing and Assessing the Findings of the Special Issue
Authors: Katsoyiannis Ioannis A. , Torretta Vincenzo
Published: February 2021

The present special issue collected articles that address the very important topic of innovative approaches in water and wastewater treatment technologies. Thirteen articles are published, ten research paper and three review articles. The papers can be divided in four major categories, namely, membrane treatment, adsorption studies, advanced oxidation processes and wastewater treatment optimization. In the editorial, a brief description of the findings of each paper is presented along with a critical assessment.
Modeling the Effects of Calcium Overload on Mitochondrial Ultrastructural Remodeling
Authors: Strubbe-Rivera Jasiel O. , Chen Jiahui , West Benjamin A. , Parent Kristin N. , Wei Guo-Wei , Bazil Jason N.
Published: February 2021

Mitochondrial cristae are dynamic invaginations of the inner membrane and play a key role in its metabolic capacity to produce ATP. Structural alterations caused by either genetic abnormalities or detrimental environmental factors impede mitochondrial metabolic fluxes and lead to a decrease in their ability to meet metabolic energy requirements. While some of the key proteins associated with mitochondrial cristae are known, very little is known about how the inner membrane dynamics are involved in energy metabolism. In this study, we present a computational strategy to understand how cristae are formed using a phase-based separation approach of both the inner membrane space and matrix space, which are explicitly modeled using the Cahn–Hilliard equation. We show that cristae are formed as a consequence of minimizing an energy function associated with phase interactions which are subject to geometric boundary constraints. We then extended the model to explore how the presence of calcium phosphate granules, entities that form in calcium overload conditions, exert a devastating inner membrane remodeling response that reduces the capacity for mitochondria to produce ATP. This modeling approach can be extended to include arbitrary geometrical constraints, the spatial heterogeneity of enzymes, and electrostatic effects to mechanize the impact of ultrastructural changes on energy metabolism.
Development of the Mesoscale Model GRAMM-SCI: Evaluation of Simulated Highly-Resolved Flow Fields in an Alpine and Pre-Alpine Region
Authors: Oettl Dietmar
Published: February 2021

In this study, new developments implemented in the mesoscale model GRAMM-SCI are presented. GRAMM-SCI has been specifically developed for providing flow fields in the sub-kilometer range. A comprehensive model evaluation using wind, temperature, radiation, as well as soil moisture and soil temperature observations in an alpine valley and in a hilly pre-alpine region in Styria (Austria) is presented. Three one-way nested model domains were used, whereby the coarse model run (5000 m horizontal resolution) was initialized and forced using ERA5 reanalysis data. The grid sizes for the two inner domains were set to 1000 m and 200 m, respectively. Comparisons were carried out for a five-day period in October 2017 which was dominated by clear-sky conditions. Though, the observations reveal quite complex flow structures governed by interactions between synoptic flow and thermally-driven local flows, GRAMM-SCI was able to reproduce the main features satisfactorily. In addition, the new version of GRAMM-SCI shows significant improvement with regard to simulated air temperature compared with the previous one. Finally, microscale flow-field simulations were carried out for some monitoring sites that are apparently influenced by nearby buildings or vegetation.
Impact Features Induced by Single Fast Ions of Different Charge-State on Muscovite Mica
Authors: Alencar Igor , Silva Marcos R. , Leal Rafael , Grande Pedro L. , Papaléo Ricardo M.
Published: February 2021

The influence of the charge state q on surface modifications induced by the impact of individual fast, heavy ions on muscovite mica was investigated. Beams of 593 MeV 197Auq+ with well-defined initial charge states over a relatively broad range of values (30 to 51) and at different irradiation geometries were used. At normal incidence, the impact features are rounded protrusions (hillocks) with ≳20 nm in diameter. At grazing angles, besides the hillocks, craters and elongated tails (up to 350 nm-long) extending along the direction of ion penetration are produced. It is shown that the impact features at normal incidence depend strongly on the initial charge state of the projectiles. This dependence is very weak at grazing angles as the ion reaches the equilibrium charge state closer to the surface. At normal ion incidence, the hillock volume scales with q3.3 ± 0.6. This dependence stems largely from the increase in the hillock height, as a weak dependence of the diameter was observed.
Evaluation of Italian Simplified Matrix Test for Speech-Recognition Measurements in Noise
Authors: Puglisi Giuseppina Emma , di Berardino Federica , Montuschi Carla , Sellami Fatma , Albera Andrea , Zanetti Diego , Albera Roberto , Astolfi Arianna , Kollmeier Birger , Warzybok Anna
Published: February 2021

This study aimed at the evaluation of a simplified Italian matrix test (SiIMax) for speech-recognition measurements in noise for adults and children. Speech-recognition measurements with adults and children were conducted to examine the training effect and to establish reference speech-recognition thresholds of 50% (SRT50) and 80% (SRT80) correct responses. Test-list equivalency was evaluated only with adults. Twenty adults and 96 children—aged between 5 and 10 years—participated. Evaluation measurements with the adults confirmed the equivalence of the test lists, with a mean SRT50 of −8.0 dB and a standard deviation of 0.2 dB across the test lists. The test-specific slope (the average of the list-specific slopes) was 11.3%/dB, with a standard deviation of 0.6%/dB. For both adults and children, only one test list of 14 phrases needs to be presented to account for the training effect. For the adults, adaptive measurements of the SRT50 and SRT80 showed mean values of −7.0 ± 0.6 and −4.5 ± 1.1 dB, respectively. For children, a slight influence of age on the SRT was observed. The mean SRT50s were −5.6 ± 1.2, −5.8 ± 1.2 and −6.6 ± 1.3 dB for the children aged 5–6, 7–8 and 9–10 years, respectively. The corresponding SRT80s were −1.5 ± 2.7, −3.0 ± 1.7 and −3.7 ± 1.4 dB. High test–retest reliabilities of 1.0 and 1.1 dB for the SRT80 were obtained for the adults and children, respectively. This makes the test suitable for accurate and reliable speech-recognition measurements.
Small Molecule CCR4 Antagonists Protect Mice from Aspergillus Infection and Allergy
Authors: Bozza Silvia , Iannitti Rossana Giulietta , Pariano Marilena , Renga Giorgia , Costantini Claudio , Romani Luigina , Bayry Jagadeesh
Published: February 2021

The ability to regulate the recruitment of immune cells makes chemokines and their receptors attractive drug targets in many inflammatory diseases. Based on its preferential expression on T helper type 2 (Th2) cells, C-C chemokine receptor type 4 (CCR4) has been widely studied in the context of allergic diseases, but recent evidence on the expression of CCR4 in other cell types has considerably expanded the potential applications of CCR4 antagonism. However, the current number of approved indications, as well as the portfolio of CCR4-targeting drugs, are still limited. In the present study, we have assessed the potential therapeutic efficacy of a CCR4 small molecule antagonist, SP50, discovered via an in silico-based approach, against a variety of pre-clinical settings of infection with the fungus Aspergillus fumigatus. We show that SP50 efficiently worked as prophylactic vaccine adjuvant in immunocompetent mice, protected against invasive aspergillosis in immunosuppressed mice. Further, the CCR4 antagonist prevented allergic bronchopulmonary aspergillosis in susceptible mice, and in a murine model of cystic fibrosis, a genetic disorder characterized by chronic pulmonary inflammation and recurrent infections. In conclusion, our results extend the potential applications of CCR4 antagonism and prompt for the development of novel compounds with the potential to progress to clinical trials.
Diagnostic Value of Choline PET in the Preoperative Localization of Hyperfunctioning Parathyroid Gland(s): A Comprehensive Overview
Authors: Ferrari Cristina , Santo Giulia , Mammucci Paolo , Pisani Antonio Rosario , Sardaro Angela , Rubini Giuseppe
Published: February 2021

Hyperparathyroidism is a metabolic disorder characterized by the excessive production of the parathyroid hormone. The diagnosis is based on clinical and laboratory data. In most cases the only treatment is surgery and a correct preoperatory localization of the hyperfunctioning parathyroid gland(s) is essential. Currently, ultrasonography combined with [99mTc]Tc-MIBI parathyroid scintigraphy, optionally associated with single photon emission computed tomography/computed tomography (SPECT/CT), represent the standard preoperative imaging. In recent years, a number of studies have evaluated the potential role of choline positron emission tomography (PET) in hyperparathyroidism with promising results. Most of the recent evidence underlined its higher sensitivity and diagnostic accuracy in the localization of hyperfunctioning parathyroid glands. Choline PET has a higher spatial resolution that is useful for the detection of smaller parathyroid glands and it also has shorter examination times and favorable radiation exposure. These are just a few of the aspects that support it to overcome traditional imaging. Moreover, from the preliminary data, the choline uptake mechanism seems to also have an impact on its better performance. For these reasons, if first used as second level imaging in patients with negative or inconclusive traditional imaging results, several authors have supported its use as a first line investigation. This comprehensive overview aims to provide an accurate description of the preliminary results available in the literature about the use of choline PET/CT in hyperparathyroidism and to compare these results with the performance of traditional imaging methods.
Effects of Psychostimulants and Antipsychotics on Serum Lipids in an Animal Model for Schizophrenia
Authors: Correia Banny Silva Barbosa , Nani João Victor , Waladares Ricardo Raniery , Stanisic Danijela , Costa Tássia Brena Barroso Carneiro , Hayashi Mirian A. F. , Tasic Ljubica
Published: February 2021

Schizophrenia (SCZ) treatment is essentially limited to the use of typical or atypical antipsychotic drugs, which suppress the main symptoms of this mental disorder. Metabolic syndrome is often reported in patients with SCZ under long-term drug treatment, but little is known about the alteration of lipid metabolism induced by antipsychotic use. In this study, we evaluated the blood serum lipids of a validated animal model for SCZ (Spontaneously Hypertensive Rat, SHR), and a normal control rat strain (Normotensive Wistar Rat, NWR), after long-term treatment (30 days) with typical haloperidol (HAL) or atypical clozapine (CLZ) antipsychotics. Moreover, psychostimulants, amphetamine (AMPH) or lisdexamfetamine (LSDX), were administered to NWR animals aiming to mimic the human first episode of psychosis, and the effects on serum lipids were also evaluated. Discrepancies in lipids between SHR and NWR animals, which included increased total lipids and decreased phospholipids in SHR compared with NWR, were similar to the differences previously reported for SCZ patients relative to healthy controls. Administration of psychostimulants in NWR decreased omega-3, which was also decreased in the first episode of psychosis of SCZ. Moreover, choline glycerophospholipids allowed us to distinguish the effects of CLZ in SHR. Thus, changes in the lipid metabolism in SHR seem to be reversed by the long-term treatment with the atypical antipsychotic CLZ, which was under the same condition described to reverse the SCZ-like endophenotypes of this validated animal model for SCZ. These data open new insights for understanding the potential influence of the treatment with typical or atypical antipsychotics on circulating lipids. This may represent an outcome effect from metabolic pathways that regulate lipids synthesis and breakdown, which may be reflecting a cell lipids dysfunction in SCZ.
Experimental Models for the Study of Hereditary Cornification Defects
Authors: Copic Dragan , Laggner Maria , Kalinina Polina , Klas Katharina , Tschachler Erwin , Mildner Michael
Published: February 2021

Ichthyoses comprise a broad spectrum of keratinization disorders due to hereditary defects of cornification. Until now, mutations in more than 50 genes, mostly coding for structural proteins involved in epidermal barrier formation, have been identified as causes for different types of these keratinization disorders. However, due to the high heterogeneity and difficulties in the establishment of valid experimental models, research in this field remains challenging and translation of novel findings to clinical practice is difficult. In this review, we provide an overview of existing models to study hereditary cornification defects with focus on ichthyoses and palmoplantar keratodermas.
Saliency-Aware Subtle Augmentation Improves Human Visual Search Performance in VR
Authors: Lukashova-Sanz Olga , Wahl Siegfried
Published: February 2021

Visual search becomes challenging when the time to find the target is limited. Here we focus on how performance in visual search can be improved via a subtle saliency-aware modulation of the scene. Specifically, we investigate whether blurring salient regions of the scene can improve participant’s ability to find the target faster when the target is located in non-salient areas. A set of real-world omnidirectional images were displayed in virtual reality with a search target overlaid on the visual scene at a pseudorandom location. Participants performed a visual search task in three conditions defined by blur strength, where the task was to find the target as fast as possible. The mean search time, and the proportion of trials where participants failed to find the target, were compared across different conditions. Furthermore, the number and duration of fixations were evaluated. A significant effect of blur on behavioral and fixation metrics was found using linear mixed models. This study shows that it is possible to improve the performance by a saliency-aware subtle scene modulation in a challenging realistic visual search scenario. The current work provides an insight into potential visual augmentation designs aiming to improve user’s performance in everyday visual search tasks.
Brain Activation Changes While Walking in Adults with and without Neurological Disease: Systematic Review and Meta-Analysis of Functional Near-Infrared Spectroscopy Studies
Authors: Bishnoi Alka , Holtzer Roee , Hernandez Manuel E.
Published: February 2021

(1) Functional near-infrared spectroscopy (fNIRS) provides a useful tool for monitoring brain activation changes while walking in adults with neurological disorders. When combined with dual task walking paradigms, fNIRS allows for changes in brain activation to be monitored when individuals concurrently attend to multiple tasks. However, differences in dual task paradigms, baseline, and coverage of cortical areas, presents uncertainty in the interpretation of the overarching findings. (2) Methods: By conducting a systematic review of 35 studies and meta-analysis of 75 effect sizes from 17 studies on adults with or without neurological disorders, we show that the performance of obstacle walking, serial subtraction and letter generation tasks while walking result in significant increases in brain activation in the prefrontal cortex relative to standing or walking baselines. (3) Results: Overall, we find that letter generation tasks have the largest brain activation effect sizes relative to walking, and that significant differences between dual task and single task gait are seen in persons with multiple sclerosis and stroke. (4) Conclusions: Older adults with neurological disease generally showed increased brain activation suggesting use of more attentional resources during dual task walking, which could lead to increased fall risk and mobility impairments. PROSPERO ID: 235228.
Cerebrospinal Fluid Parameters in Antisense Oligonucleotide-Treated Adult 5q-Spinal Muscular Atrophy Patients
Authors: Müschen Lars Hendrik , Osmanovic Alma , Binz Camilla , Jendretzky Konstantin F. , Ranxha Gresa , Bronzlik Paul , Abu-Fares Omar , Wiehler Flavia , Möhn Nora , Hümmert Martin W. , Gingele Stefan , Götz Friedrich , Stangel Martin , Skripuletz Thomas , Schreiber-Katz Olivia , Petri Susanne
Published: February 2021

Approval of nusinersen, an intrathecally administered antisense oligonucleotide, for the treatment of 5q-spinal muscular atrophy (SMA) marked the beginning of a new therapeutic era in neurological diseases. Changes in routine cerebrospinal fluid (CSF) parameters under nusinersen have only recently been described in adult SMA patients. We aimed to explore these findings in a real-world setting and to identify clinical and procedure-associated features that might impact CSF parameters. Routinely collected CSF parameters (leukocyte count, lactate, total protein, CSF/serum albumin quotient (QAlbumin), oligoclonal bands) of 28 adult SMA patients were examined for up to 22 months of nusinersen treatment. Total protein and QAlbumin values significantly increased in the first 10 months, independent of the administration procedure. By month 14, no further increases were detected. Two patients developed transient pleocytosis. In two cases, positive oligoclonal bands were found in the beginning and in four patients throughout the whole observation period. No clinical signs of inflammatory central nervous system disease were apparent. Our data confirm elevated CSF total protein and QAlbumin during nusinersen treatment. These alterations may be caused by both repeated lumbar punctures and the interval between procedures rather than by the medication itself. Generally, there were no severe alterations of CSF routine parameters. These results further underline the safety of nusinersen therapy.
Global Performance of Sustainable Thermal Insulating Systems with Cork for Building Facades
Authors: Malanho Sofia , Veiga Rosário , Farinha Catarina Brazão
Published: February 2021

Rehabilitation of facades may be carried out with the application of External Thermal Insulation Systems (ETICS). Their main contribution is the increase of the energy efficiency of buildings. In the literature, hygrothermal, impact and fire performance studies have been carried out on several systems with different insulation materials, such as expanded polystyrene, mineral wool and extruded polystyrene foam insulation. Due to the growing concern with the environment, systems are being developed with more sustainable and ecological materials, such as ICB (expanded cork). These type of boards are responsible for a negative impact in global warming potential, significantly improving the environmental benefits of their use. As these systems were recently introduced to the market, applications on site are very recent and their behaviour over time still unknown. In this research, the durability and global performance of more sustainable systems (with ICB) were analysed through an experimental campaign and compared with EPS (expanded polystyrene) systems. The results show that the systems with ICB obtained satisfactory global behaviour comparable with the EPS systems. The ICB sustainable systems analysed stood out in acoustic performance.
Micro-Mechanical and 3D Fractal Analysis, Durability, and Thermal Behaviour of Nano-Modified Cementitious Lightweight Composites for Building Facades
Authors: Blankson Marva , Erdem Savaş , Gürbüz Ezgi
Published: February 2021

There are increasing research endeavours on the application of nanotechnology in the construction industry and lightweight composites. In this study, the influence of different percentage (1%, 2%, and 3% by weight of cement) colloidal nano-silica particles on the mechanical, thermal, and durability properties of lightweight cementitious composites was studied through measurement of compressive strength, flexural response, micro-hardness measurement, pore structure analysis, thermal conductivity, water permeability, and chloride penetration. Moreover, 3D X-ray Compute Tomography together with digital image analysis and 3D fractal analysis was used to characterize the nano-silica, micro-structures, and the fracture surfaces. The experimental results show that incorporating nano-silica particles resulted in a mechanical strength increase up to 45.4 % and a water permeability and chloride migration decrease up to 51.2% and 48.2%, respectively. The micro-structural and 3D fractal analysis also indicated that dense, flaw-free, and thus more resistant, interfaces to micro-cracks were formed and greater fractal dimensions were obtained with the increase of the nano-silica content. Finally, the 3D views confirmed that the nano-silica clusters were well interconnected which further increase the carrying capacity and reducing the heat flow.
Feedforward Artificial Neural Network-Based Colorectal Cancer Detection Using Hyperspectral Imaging: A Step towards Automatic Optical Biopsy
Authors: Jansen-Winkeln Boris , Barberio Manuel , Chalopin Claire , Schierle Katrin , Diana Michele , Köhler Hannes , Gockel Ines , Maktabi Marianne
Published: February 2021

Currently, colorectal cancer (CRC) is mainly identified via a visual assessment during colonoscopy, increasingly used artificial intelligence algorithms, or surgery. Subsequently, CRC is confirmed through a histopathological examination by a pathologist. Hyperspectral imaging (HSI), a non-invasive optical imaging technology, has shown promising results in the medical field. In the current study, we combined HSI with several artificial intelligence algorithms to discriminate CRC. Between July 2019 and May 2020, 54 consecutive patients undergoing colorectal resections for CRC were included. The tumor was imaged from the mucosal side with a hyperspectral camera. The image annotations were classified into three groups (cancer, CA; adenomatous margin around the central tumor, AD; and healthy mucosa, HM). Classification and visualization were performed based on a four-layer perceptron neural network. Based on a neural network, the classification of CA or AD resulted in a sensitivity of 86% and a specificity of 95%, by means of leave-one-patient-out cross-validation. Additionally, significant differences in terms of perfusion parameters (e.g., oxygen saturation) related to tumor staging and neoadjuvant therapy were observed. Hyperspectral imaging combined with automatic classification can be used to differentiate between CRC and healthy mucosa. Additionally, the biological changes induced by chemotherapy to the tissue are detectable with HSI.
A Review of Circulating Tumour Cell Enrichment Technologies
Authors: Rushton Amelia J. , Nteliopoulos Georgios , Shaw Jacqueline A. , Coombes R. Charles
Published: February 2021

Circulating tumour cells (CTCs) are the precursor cells for the formation of metastatic disease. With a simple blood draw, liquid biopsies enable the non-invasive sampling of CTCs from the blood, which have the potential to provide important insights into cancer detection and monitoring. Since gaining FDA approval in 2004, the CellSearch system has been used to determine the prognosis of patients with metastatic breast, prostate and colorectal cancers. This utilises the cell surface marker Epithelial Cell Adhesion Molecule (EpCAM), to enrich CTCs, and many other technologies have adopted this approach. More recently, the role of mesenchymal-like CTCs in metastasis formation has come to light. It has been suggested that these cells are more aggressive metastatic precursors than their epithelial counterparts; however, mesenchymal CTCs remain undetected by EpCAM-based enrichment methods. This has prompted the development of a variety of ‘label free’ enrichment technologies, which exploit the unique physical properties of CTCs (such as size and deformability) compared to other blood components. Here, we review a wide range of both immunocapture and label free CTC enrichment technologies, summarising the most significant advantages and disadvantages of each. We also highlight the important characteristics that technologies should possess for routine clinical use, since future developments could have important clinical implications, with the potential to direct personalised therapies for patients with cancer.
Novel CD37, Humanized CD37 and Bi-Specific Humanized CD37-CD19 CAR-T Cells Specifically Target Lymphoma
Authors: Golubovskaya Vita , Zhou Hua , Li Feng , Valentine Michael , Sun Jinying , Berahovich Robert , Xu Shirley , Quintanilla Milton , Ma Man Cheong , Sienkiewicz John , Huang Yanwei , Wu Lijun
Published: February 2021

CD19 and CD37 proteins are highly expressed in B-cell lymphoma and have been successfully targeted with different monotherapies, including chimeric antigen receptor (CAR)-T cell therapy. The goal of this study was to target lymphoma with novel CD37, humanized CD37, and bi-specific humanized CD37-CD19 CAR-T cells. A novel mouse monoclonal anti-human CD37 antibody (clone 2B8D12F2D4) was generated with high binding affinity for CD37 antigen (KD = 1.6 nM). The CD37 antibody specifically recognized cell surface CD37 protein in lymphoma cells and not in multiple myeloma or other types of cancer. The mouse and humanized CD37-CAR-T cells specifically killed Raji and CHO-CD37 cells and secreted IFN-gamma. In addition, we generated bi-specific humanized hCD37-CD19 CAR-T cells that specifically killed Raji cells, CHO-CD37, and Hela-CD19 cells and did not kill control CHO or Hela cells. Moreover, the hCD37-CD19 CAR-T cells secreted IFN-gamma against CD37-positive and CD19-positive target CHO-CD37, Hela-CD19 cells, respectively, but not against CD19 and CD37-negative parental cell line. The bi-specific hCD37-CD19 significantly inhibited Raji xenograft tumor growth and prolonged mouse survival in NOD scid gamma mouse (NSG) mouse model. This study demonstrates that novel humanized CD37 and humanized CD37-CD19 CAR-T cells specifically targeted either CD37 positive or CD37 and CD19-positive cells and provides a basis for future clinical studies.
Fatigue in Cancer Patients in Palliative Care—A Review on Pharmacological Interventions
Authors: Klasson Caritha , Helde Frankling Maria , Lundh Hagelin Carina , Björkhem-Bergman Linda
Published: February 2021

Fatigue is one of the most distressing symptoms experienced by cancer patients. The suggested biological mechanism for cancer related fatigue (CRF) includes immune activation triggered by tumor tissue or by anticancer treatment but other mechanisms have also been proposed. Previous large meta-analysis of interventions on fatigue focuses mostly on patients early in the disease trajectory, with only one tenth of included studies performed in palliative cohorts. The aim of this narrative review is therefore to present a background on CRF with focus on the palliative setting. A summary of recent randomized, controlled trials on pharmacological interventions on CRF in palliative care is presented, including studies on psychostimulants, corticosteroids, testosterone and melatonin. Interestingly, in several of these studies there was a positive and similar effect on fatigue in both the intervention and the placebo arm—indicating an important placebo effect for any pharmacological treatment. In addition, studies on dietary supplements and on pharmacological complementary medicines are discussed. To conclude, the evidence is still weak for using pharmacological treatments on CRF in palliative care patients—although methylphenidate and corticosteroids might be considered.
Effects of Hydrothermal Aging on CO and NO Oxidation Activity over Monometallic and Bimetallic Pt-Pd Catalysts
Authors: Schütz Jochen , Störmer Heike , Lott Patrick , Deutschmann Olaf
Published: February 2021

By combining scanning transmission electron microscopy, CO chemisorption, and energy dispersive X-ray spectroscopy with CO and NO oxidation light-off measurements we investigated deactivation phenomena of Pt/Al2O3, Pd/Al2O3, and Pt-Pd/Al2O3 model diesel oxidation catalysts during stepwise hydrothermal aging. Aging induces significant particle sintering that results in a decline of the catalytic activity for all catalyst formulations. While the initial aging step caused the most pronounced deactivation and sintering due to Ostwald ripening, the deactivation rates decline during further aging and the catalyst stabilizes at a low level of activity. Most importantly, we observed pronounced morphological changes for the bimetallic catalyst sample: hydrothermal aging at 750 °C causes a stepwise transformation of the Pt-Pd alloy via core-shell structures into inhomogeneous agglomerates of palladium and platinum. Our study shines a light on the aging behavior of noble metal catalysts under industrially relevant conditions and particularly underscores the highly complex transformation of bimetallic Pt-Pd diesel oxidation catalysts during hydrothermal treatment.
MOF Embedded and Cu Doped CeO2 Nanostructures as Efficient Catalyst for Adipic Acid Production: Green Catalysis
Authors: Bibi Shabahat , Pervaiz Erum , Yang Minghui , Rabi Osama
Published: February 2021

Greatly efficient chemical processes are customarily based upon a catalyst activating the process pathway to achieve higher yields of a product with desired specifications. Catalysts capable of achieving good performance without compromising green credentials are a pre-requisite for the development of a sustainable process. In this study, CeO2 nanoparticles were tested for their catalytic activity with two different configurations, one as a hybrid of CeO2 nanoparticles with Zeolitic Immidazole Framework (ZIF-67) and second being doped Cu cations into CeO2 nanoparticles. Physicochemical and catalytic activity was investigated and compared for both systems. Each hybrid was synthesized by embedding the CeO2 nanoparticles into the microporous structure of ZIF-67, and Cu doped CeO2 nanoparticles were prepared by a facile hydrothermal route. As a catalytic test, it was employed for the oxidation of cyclohexene to adipic acid (AA) as an alternative to expensive noble metal-based catalysts. Heterogeneous ZIF-67/CeO2 found catalytical activity towards the oxidation of cyclohexene with nearly complete conversion of cyclohexene into AA under moderate and co-catalyst free reaction conditions, whereas Cu doped CeO2 nanoparticles have shown no catalytic activity towards cyclohexene conversion, depicting the advantages of the porous ZIF-67 structure and its synergistic effect with CeO2 nanoparticles. The large surface area catalyst could be a viable option for the green synthesis of many other chemicals.
Recent Progress in Synthesis and Application of Nanosized and Hierarchical Mordenite—A Short Review
Authors: Kalvachev Yuri , Todorova Totka , Popov Cyril
Published: February 2021

Zeolites with their unique properties find applications in various fields, including medicine, agronomy, ecology, production of detergents and drying agents, and in a number of industrial processes. Among zeolites, mordenite is particularly widespread because of its high silica/alumina ratio, which allows it to resist exposure to high temperatures and to acidic gases and liquids. Mordenite is commercially available as a natural mineral and as a synthesized material. This zeolite is mostly used in its synthetic form as an acid catalyst in the petrochemical industry for the isomerization of alkanes and aromatics. In this review, we consider the scientific literature on the structure, synthesis, and two main types of modifications that solve the diffusion difficulties during catalytic processes. The first type of modifications is related to a reduction of the size of the mordenite crystals obtained to submicron or nanometric range, whereas the second ones aim to obtain hierarchical mordenite samples by appropriate post-synthetic treatments. Both types of modifications find many other applications besides solving diffusion constraints in catalytic processes. Attempts to fine-tune and control the particle size in the first type of modifications or the pore size in the second ones by adjusting various parameters during the synthesis are described.
The Efficacy of Silver Nitrate (AgNO3) as a Coating Agent to Protect Paper against High Deteriorating Microbes
Authors: Fouda Amr , Abdel-Maksoud Gomaa , Saad Hosam A. , Gobouri Adil A. , Mohammedsaleh Zuhair M. , Abdel-Haleem El-Sadany Mohamad
Published: February 2021

This study focuses on the efficacy of silver nitrate (AgNO3) as a coating agent used to preserve papers against microbial deterioration. To this end, the in vitro cytotoxicity of AgNO3 was assessed against two normal cell lines, WI-38 and HFB-4, to detect a safe dose that can be used as a coating agent, which was 80 µg mL−1. Bacillus subtilis B3 and Penicillium chrysogenum F9 were selected as high deteriorating microbes, previously isolated from a historical manuscript dating back to 1677 A.-D. The microbial growth inhibition, color change, mechanical properties, and cellulosic fibers of untreated/treated papers were evaluated. The data showed the efficacy of AgNO3 to inhibit the growth of B. subtilis with a percentage of 100% after 7 days, while it inhibits the growth of P. chrysogenum with a percentage of 85.9 ± 1.1% after 21 days. The color and mechanical properties of treated paper in the presence/absence of microbial inoculation were slightly changed, although they changed greatly due to microbial growth in the absence of AgNO3. The EDX analysis confirmed the successful adsorption of Ag-ion on papers, with a weight percentage of 1.9%. The cellulosic fibers of untreated paper in the presence of microbial growth were highly deteriorated as compared with treated and standard filter paper (shown by FT-IR and SEM).