bluuebunny/crossref_metadata_2025 huggingface dataset:
Afforestation Influences Soil Aggregate Stability by Regulating Aggregate Transformation in Karst Rocky Desertification Areas
Authors: Zhu Dayun , Yang Qian , Zhao Yingshan , Cao Zhen , Han Yurong , Li Ronghan , Ni Ju , Wu Zhigao
Published: June 2023

Surface vegetation has a substantial impact on soil aggregate stability, which is an important indicator of soil quality. However, there is still limited research on the response of soil aggregate stability indicators and the organic carbon, total nitrogen, and total phosphorus content in soil aggregates for different vegetation patterns in rocky desertification fragile ecological areas. Therefore, in order to study the effects of different vegetation restoration models on soil aggregate stability and aggregate related nutrient content and their promoting relationships in the karst rocky desertification areas in southwest China, soil samples under three artificial restoration vegetation measures (Juglans regia L.-Rosa roxburghii Tratt., Rosa roxburghii Tratt.-Lolium perenne L., Juglans regia L.-Lolium perenne L.) were collected in 0–10 cm and 10–20 cm soil, and the traditional farmland (Zea mays L.) was used as the control, combined with dry and wet sieving experiments for the research and analysis. The results showed that there were significant differences in the distribution of aggregates and soil nutrients among the four types of plots. Compared with traditional agricultural land, artificial afforestation increased the content of soil large macroaggregates (LMAs) and decreased the proportion of microaggregates (MIAs) and silt+clay (SCA), which enhanced the soil aggregate stability and reduced the soil fragmentation and erodibility. The afforestation restoration increased the content of soil aggregate-related SOC, TN, and TP, and increased with the decrease in the aggregate particle size. Research has found that soil aggregate stability indicators are significantly influenced by the particle size distribution of soil aggregates. In the positive succession process of vegetation types, soil nutrient accumulation is controlled by changes in the soil aggregate particle size, which affects the soil aggregate stability and reduces soil erodibility, thereby protecting the soil nutrient loss. The composite management of forest and irrigation in degraded ecological areas has certain reference and indicative significance for ecological restoration in rocky desertification areas.
Assessment of Energy, Dynamic and Economic Balance of Chipping Operation in Poplar Medium Rotation Coppice (MRC) Plantations
Authors: Stefanoni Walter , Fanigliulo Roberto , Pochi Daniele , Fornaciari Laura , Grilli Renato , Benigni Stefano , Latterini Francesco , Sperandio Giulio
Published: June 2023

Exploiting renewable energy sources is one of the main strategies defined by the EU to overcome dependence on foreign markets for energy supply. Wood fuel sourced from the agroforestry sector can contribute significantly to achieving the goal, though its economic and environmental sustainability is intimately dependent on proper harvesting and chipping operations. In the present article, both economic and environmental aspects of Medium Rotation Coppice (MRC) were investigated regarding chipping. A small-scale chipper and tractor were equipped with real-time sensors to monitor time, t (s); fuel consumption, F (cm3); PTO torque, M (daNm); PTO speed, s (min−1); and stem diameter, D (mm) during the comminution of 61 poplar plants (gathered in 5 classes according to trunk diameter) grown in MRC system. More than 29,000 records were taken and analyzed. Predictive models for working time, working productivity, CO2 emission, energy consumption, fuel consumption and costs were also produced. Higher diametric classes exhibited lower fuel consumption, less CO2 emission and less energy demand during chipping. Time and operating costs were statistically different among classes, with minimum values of 0.22 (SD ± 0.02) h·Mg−1 and 12.07 (SD ± 0.93) €·Mg−1 in class 5 and maximum values of 0.64 (SD ± 0.09) h·Mg−1 and 35.34 (SD ± 4.88) €·Mg−1 in class 1, respectively. Fuel consumption ranges from 3.04 (SD ± 0.88) L·Mg−1 in class 5 to 7.32 (SD ± 1.46) L·Mg−1 in class 1. The lowest CO2 emission of 8.03 (SD ± 2.32) kg·Mg−1 was found class 5. However, the total cost of coppice production did not exceed large-scale MRC production due to the lower purchase price of the machinery involved. Eventually, predictive models showed high reliability as estimating tools for important variables, such as working time, working productivity, CO2 emissions, energy consumption, fuel consumption and costs.
Metal Salt-Based Deep Eutectic Solvent Pretreatment of Moso Bamboo to Improve Enzymatic Hydrolysis
Authors: Chen Tianying , Guo Guixin , Shen Da , Tang Yanjun
Published: June 2023

Research on metal salt-based deep eutectic solvent (DES) pretreatment is still in its infancy, and the effect of hydroxyl groups on Cl− in choline chloride (ChCl) is not resolved. In this study, a type IV DES composed of metal salt and glycerol (Gly) was prepared for pretreatment of moso bamboo to improve its enzymatic hydrolysis. The correlation between enzymatic hydrolysis and the contents of hemicelluloses and lignin was evaluated using the Box–Behnken design. The results showed that FeCl3-based DES was optimal among various DES. The solid recovery was reduced to 55.54% following FeCl3/Gly pretreatment, which was effective in the removal of hemicelluloses and lignin compared with ternary DES pretreatment (with ChCl) under mild conditions (100 °C, 3 h). With the increase of pretreatment temperature (120 °C, 2 h), a significant proportion of hemicelluloses (76.07%) and lignin (62.77%) was removed. The structure of FeCl3/Gly pretreatment residue was seriously damaged, and the glucose yield increased to 91.13% following enzymatic hydrolysis. This correlation indicated that the hemicelluloses’ content had a significant influence on enzymatic hydrolysis of the residue following FeCl3/Gly pretreatment. This study elucidates the pretreatment effect of metal salt-based DES, which will be helpful in the value-added conversion of moso bamboo under mild conditions.
Comparative Analysis of Microbial and Mycotoxin Contamination in Korean Traditional Soybean Paste and Soy Sauce Production with and without Starter
Authors: Kim Jinkwi , Jeong Jiyoun , Jang Mi , Kim Jong-Chan , Lee Heeyoung
Published: June 2023

Doenjang and ganjang are traditional, Korean fermented foods. They are manufactured by fermenting jangs, either through the traditional natural fermentation, using straw, or the commercial inoculating starter cultures. However, both Bacillus cereus and aflatoxins have been detected in jangs, compromising their safety. Aflatoxins have been detected on numerous occasions. However, studies are yet to be conducted on whether these safety issues differ depending on the manufacturing method. In this study, we evaluated whether the manufacturing methods of doenjang and ganjang alter their safety. Samples of traditional and commercial doenjang and ganjang were analyzed for aflatoxin and B. cereus contamination. Microbiome taxonomic profiling was performed to assess microbial composition. The experimental methodology involved sample collection from various stages of production, including the use of starter cultures and natural fermentation processes. Aflatoxin levels were determined using regulatory limits, and B. cereus content was evaluated against specific thresholds. Aflatoxins were detected in both traditional and commercial doenjang and ganjang, with either the total aflatoxin (15 μg/kg) or aflatoxin B1 (10 μg/kg) exceeding the regulatory limits. However, ochratoxin A was not detected in any of the samples. B. cereus was detected in some samples, within the regulatory limit (4 log CFU/g), and was not influenced by the manufacturing method. Analysis at the production stage showed that aflatoxin increased alongside the fermentation time in traditional doenjang. However, in ganjang, no significant differences were associated with the fermentation period. When using starter cultures, the fermentation period did not affect the toxin level in both foods. Both methods showed lower aflatoxin content in the initial doenjang and ganjang samples than in meju. B. cereus was not detected in either method, as its content decreased over the fermentation period. Microbiome taxonomic profiling confirmed that even when using starter cultures, B. cereus was not a dominant species and was considerably affected by the environment. Staphylococcus aureus and Pseudomonas, pathogenic in nature, were detected in products manufactured using the traditional method; hence, the potential risk of this method was higher than that of the commercial method. The experimental methodology employed in this study contributes to understanding the microbial composition and toxin contamination levels in doenjang and ganjang, contributing to the overall knowledge of their safety and quality control.
Experimental Prototype of Electromagnetic Emissions for Biotechnological Research: Monitoring Cocoa Bean Fermentation Parameters
Authors: Guzmán-Armenteros Tania María , Ruales Jenny , Villacís-Chiriboga José , Guerra Luis Santiago
Published: June 2023

A Helmholtz-type electromagnetic emission device, which uses an oscillating magnetic field (OMF), with potential applications in biotechnological research, was built and validated. The coils were connected to an alternating current (AC) generator to generate a 0.5 to 110 mT field at their center. OMF measurements were performed with a Hall effect sensor with a digital signal connection (Arduino nano) and data output to a PC using LabVIEW v2017SP1 software. The fermentation process of the cocoa bean variety CCN 51, exposed to four levels of OMF density for 60 min (0, 5, 40, and 80 mT/60 min), was analyzed. Different variables of the grain fermentation process were evaluated over six days. The ANOVA test probed the device’s linearity, accuracy, precision, repeatability, reliability, and robustness. Moreover, CCN 51 cocoa beans’ EMF-exposure effect was evaluated under different OMF densities for 60 min. The results show the validity of the equipment under working conditions and the impact of EMF (electromagnetic fields) on the yield, deformation, and pH of cocoa beans. Thus, we concluded that the operation of the prototype is valid for use in biotechnological studies.
Developing a Clean Labelled Snack Bar Rich in Protein and Fibre with Dry-Fractionated Defatted Durum Wheat Cake
Authors: Squeo Giacomo , Latrofa Vittoria , Vurro Francesca , De Angelis Davide , Caponio Francesco , Summo Carmine , Pasqualone Antonella
Published: June 2023

The shift towards a vegetarian, vegan, or flexitarian diet has increased the demand for vegetable protein and plant-based foods. The defatted cake generated during the extraction of lipids from durum wheat (Triticum turgidum L. var. durum) milling by-products is a protein and fibre-containing waste, which could be upcycled as a food ingredient. This study aimed to exploit the dry-fractionated fine fraction of defatted durum wheat cake (DFFF) to formulate a vegan, clean labelled, cereal-based snack bar. The design of experiments (DoEs) for mixtures was applied to formulate a final product with optimal textural and sensorial properties, which contained 10% DFFF, 30% glucose syrup, and a 60% mix of puffed/rolled cereals. The DFFF-enriched snack bar was harder compared to the control without DFFF (cutting stress = 1.2 and 0.52 N/mm2, and fracture stress = 12.9 and 9.8 N/mm2 in the DFFF-enriched and control snack bar, respectively), due to a densifying effect of DFFF, and showed a more intense yellow hue due to the yellow–brownish colour of DFFF. Another difference was in the caramel flavour, which was more intense in the DFFF-enriched snack bar. The nutritional claims “low fat” and “source of fibre” were applicable to the DFFF-enriched snack bar according to EC Reg. 1924/06.
High Oxygen Shocking Reduces Postharvest Disease and Maintains Satisfying Quality in Fresh Goji Berries during Cold Storage by Affecting Fungi Community Composition
Authors: Hou Shuangdi , Zhang Gaopeng , Zhao Wei , Zheng Jiaxuan , Xue Min , Fan Yanli , Liu Xia
Published: June 2023

Fresh goji (Lycium barbarum L.) berries were treated with high-concentration (50% and 90%) oxygen shocking for 30 min and then stored at 0 ± 0.5 °C for 30 d. Decay, aerobic plate count, firmness, weight loss, total soluble solid (TSS), and titratable acidity (TA) were evaluated during storage. A total of 90% O2 shocking more effectively reduced decay and maintained the weight loss and firmness of goji berries. Subsequently, changes in fungi communities were analyzed using high-throughput sequencing (HTS) in the 90% O2-shocking and control groups. The results showed that 90% O2 shocking retained the richness and diversity of fungi communities and the microbiome was related to the quality properties of the fruit. Thus, we inferred that high oxygen shocking inhibited the development of natural decay and maintained the satisfying quality of goji berries by affecting the fungi community composition, which reduced the growth of pathogenic fungi and harmful saprotrophic fungi in the genera, such as Filobasidium sp., Alternaria sp., and Cladosporium sp. We provide a new insight into the disease development and quality changes during the storage of postharvest goji berries.
Predicting the Potential Impact of Emergency on Global Grain Security: A Case of the Russia–Ukraine Conflict
Authors: Xu Yuan , Wang Zhongxiu , Dong Wenjie , Chou Jieming
Published: June 2023

Global emergencies have a profound impact on exacerbating food insecurity, and the protracted Russia–Ukraine conflict has emerged as a significant driver of a global food crisis. Accurately quantifying the impact of this conflict is crucial for achieving sustainable development goals. The multi-indicator comprehensive evaluation approach was used to construct a grain security composite index (GSCI). Moreover, econometric model was used to predict the potential impacts of the conflict on global grain security in 2030 under two scenarios: with and without the “Russia-Ukraine conflict”. The results conclude that global food prices reached unprecedented levels as a consequence of the conflict, leading to notable fluctuations in food prices, especially with a significant surge in wheat prices. The conflict had a negative impact on global grain security, resulting in a decline in grain security from 0.538 to 0.419. Predictions indicate that the influence of the conflict on global grain security will be substantially greater compared to the scenario without the conflict in 2023–2030, ranging from 0.033 to 0.13. Furthermore, grain security will first decrease and then increase under the sustained consequences of the conflict. The achievement of the 2030 sustainable development goals will encounter significant challenges in light of these circumstances.
Assessing the Risk of Seasonal Effects of Campylobacter Contaminated Broiler Meat Prepared In-Home in the United States
Authors: Xu Xinran , Rothrock Michael J. , Dev Kumar Govindaraj , Mishra Abhinav
Published: June 2023

Campylobacter has consistently posed a food safety issue in broiler meat. This study aimed to create a quantitative microbial risk assessment model from retail to consumption, designed to evaluate the seasonal risk of campylobacteriosis associated with broiler meat consumption in the United States. To achieve this, data was gathered to build distributions that would enable us to predict the growth of Campylobacter during various stages such as retail storage, transit, and home storage. The model also included potential fluctuations in concentration during food preparation and potential cross-contamination scenarios. A Monte Carlo simulation with 100,000 iterations was used to estimate the risk of infection per serving and the number of infections in the United States by season. In the summer, chicken meat was estimated to have a median risk of infection per serving of 9.22 × 10−7 and cause an average of about 27,058,680 infections. During the winter months, the median risk of infection per serving was estimated to be 4.06 × 10−7 and cause an average of about 12,085,638 infections. The risk assessment model provides information about the risk of broiler meat to public health by season. These results will help understand the most important steps to reduce the food safety risks from contaminated chicken products.
Sweeteners’ Influence on In Vitro α-Glucosidase Inhibitory Activity, Cytotoxicity, Stability and In Vivo Bioavailability of the Anthocyanins from Lingonberry Jams
Authors: Scrob Teodora , Filip Gabriela Adriana , Baldea Ioana , Varodi Sânziana Maria , Cimpoiu Claudia
Published: June 2023

Several lines of evidence demonstrate the multiple health-promoting properties of anthocyanins, but little is known regarding the bioavailability of these phytochemicals. Therefore, the stability during storage and bioavailability of anthocyanins from lingonberries jams were determined by HPLC, together with the impact of used sweeteners on their adsorption. Further, the in vitro α-glucosidase inhibition using spectrophotometric methods and cytotoxicity determined on normal and colon cancer cells were communicated. The content of anthocyanins was significantly decreased during storage in coconut sugar-based jam, but was best preserved in jam with fructose and stevia. Fructose and stevia-based jams showed the highest inhibition activity upon α-glucosidase. Lingonberry jams showed no cytotoxic effects on normal cells, but at low concentration reduced the tumor cells viability. Anthocyanins were still detectable in rats’ blood streams after 24 h, showing a prolonged bioavailability in rats. This study brings important results that will enable the development of functional food products.
Use of Essential Oil Emulsions to Control Escherichia coli O157:H7 in the Postharvest Washing of Lettuce
Authors: Pizzo Jessica Santos , Pelvine Raira Andrade , da Silva Andre Luiz Biscaia Ribeiro , Mikcha Jane Martha Graton , Visentainer Jesui Vergilio , Rodrigues Camila
Published: June 2023

Essential oils (EOs) have strong antibacterial properties and can be potential sanitizers to reduce pathogen load and prevent cross-contamination during postharvest washing. The objective of this study was to investigate the efficacy of emulsions containing oregano (OR; Origanum vulgare) and winter savory (WS; Satureja montana) EOs at different concentrations (0.94 and 1.88 µL/mL) and storage times (0 h, 24 h, and 7 days), in reducing Escherichia coli O157:H7 on the surface of three types of lettuce (romaine, crisphead, and butterhead). The EO emulsions were compared with one no-rinse treatment and three rinse treatments using water, 200 ppm chlorine, and 80 ppm peroxyacetic acid (PAA), respectively, in a simulated washing system. The results showed that while the EO emulsions significantly reduced E. coli O157:H7 on crisphead lettuce over time, not all treatments were effective for romaine and butterhead lettuce. The mixture of OR and WS at concentrations of 0.94 and 1.88 µL/mL was found to be the most effective in reducing E. coli O157:H7 on inoculated lettuce, resulting in reductions of 3.52 and 3.41 log CFU/g, respectively. Furthermore, the PAA and the mixture of OR and WS at 1.88 µL/mL effectively limited bacterial cross-contamination close to the detection limit for all lettuce types during all storage times. These results suggest that OR and WS EOs could serve as potential alternatives to chemical sanitizers for postharvest lettuce washing.
Green Synthesis of Silver Nanoparticles Loaded Hydrogel for Wound Healing; Systematic Review
Authors: Aldakheel Fahad M. , Sayed Marwa M. El , Mohsen Dalia , Fagir Mohammed H. , El Dein Dalia K.
Published: June 2023

Wound healing is a biological process that involves a series of consecutive process, and its impairment can lead to chronic wounds and various complications. Recently, there has been a growing interest in employing nanotechnology to enhance wound healing. Silver nanoparticles (AgNPs) have expanded significant attention due to their wide range of applications in the medical field. The advantages of AgNPs include their easy synthesis, change their shape, and high surface area. Silver nanoparticles are very efficient for topical drug administration and wound healing because of their high ratio of surface area to volume. The efficiency of AgNPs depends on the synthesis method and the intended application. Green synthesis methods offer an eco-friendly approach by utilizing natural sources such as plant extracts and fungus. The characterization of nanoparticles plays an important character, and it is accomplished through the use of several characterization methods such as UV-Vis spectroscopy, Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (XRD). These techniques are employed to confirm the specific characters of the prepared Silver Nanoparticles. Additionally, the review addresses the challenges and future perspectives of utilizing green-synthesized AgNPs loaded in Polyacrylamide hydrogel for wound healing applications, including the optimization of nanoparticle size, and release kinetics. Overall, this review highlights the potential of green-synthesized AgNPs loaded in Polyacrylamide hydrogel as promising for advanced wound healing therapies. There are different approaches of usage of AgNPs for wound healing such as polyacrylamide -hydrogels, and the mechanism after their antibacterial action, have been exposed.
Evaluation of Physicochemical Properties of a Hydrocolloid-Based Functional Food Fortified with Caulerpa lentillifera: A D-Optimal Design Approach
Authors: Nasir Nor Atikah Husna Ahmad , Yuswan Mohd Hafis , Shah Nor Nadiah Abd Karim , Abd Rashed Aswir , Kadota Kazunori , Yusof Yus Aniza
Published: June 2023

This study introduced a D-optimal design mixture to assess the physicochemical properties of a hydrocolloid-based functional food fortified with C. lentillifera. The combination incorporated vital jelly constituents, including extract (10–15%), sweeteners (20–29%), gelling agents (k-carrageenan and locust bean gum (LBG)), and preservatives (0–0.05%). The dependent variables were pH, Total Soluble Solid (TSS) value, and moisture content (MS). By employing the D-optimal design approach, a quadratic polynomial model was developed, demonstrating strong correlations with the experimental data with coefficient determinations (R2) of 0.9941, 0.9907, and 0.9989 for pH, TSS, and MS, respectively. Based on the D-optimal design, the study identified the optimum combination of significant factors with a desirability of 0.917, comprising 14.35% extract, 23.00% sucrose, 21.70% fructose, 26.00% k-carrageenan, 13.00% LBG, 1.95% CaCl2, and 0% methylparaben. The percentage of residual standard error (RSE) was less than 5%, indicating the reliability of the developed model. Furthermore, color analysis revealed significant differences among the jellies (p < 0.05). HPLC analysis demonstrated that the total sugar content in the fortified jellies was 28% lower compared to commercial jelly. Meanwhile, the bitterness level according to e-tongue showed a reduction of up to 90.5% when compared to the extract. These findings provide a valuable benchmark for the development of functional food products, ensuring their quality, safety, and extended shelf-life.
The Dynamics of Gene Expression Unraveling the Immune Response of Macrobrachium rosenbergii Infected by Aeromonas veronii
Authors: Peng Xin , Lan Xuan , Zhong Zhenxiao , Tu Haihui , Yao Xinyi , Tang Qiongying , Xia Zhenglong , Yang Guoliang , Yi Shaokui
Published: June 2023

To further investigate the immune response of Macrobrachium rosenbergii against Aeromonas veronii, comparative transcriptomic analyses of the M. rosenbergii hepatopancreas were conducted on challenge and control groups at 6, 12, and 24 h post-infection (hpi), independently. A total of 51,707 high-quality unigenes were collected from the RNA-seq data, and 8060 differentially expressed genes (DEGs) were discovered through paired comparisons. Among the three comparison groups, a KEGG pathway enrichment analysis showed that 173 immune-related DEGs were considerably clustered into 28 immune-related pathways, including the lysosome, the phagosome, etc. Moreover, the expression levels of the four key immune-related genes (TOLL, PAK1, GSK3β, and IKKα) were evaluated at various stages following post-infection in the hepatopancreas, hemolymph, and gills. Both PAK1 and GSK3β genes were highly up-regulated in all three tissues at 6 hpi with A. veronii; TOLL was up-regulated in the hepatopancreas and hemolymph but down-regulated in the gill at 6 hpi, and IKKα was up-regulated in hemolymph and gill, but down-regulated in the hepatopancreas at 6 hpi. These findings lay the groundwork for understanding the immune mechanism of M. rosenbergii after contracting A. veronii.
Alternative Splicing, RNA Editing, and the Current Limits of Next Generation Sequencing
Authors: Piazzi Manuela , Bavelloni Alberto , Salucci Sara , Faenza Irene , Blalock William L.
Published: June 2023

The advent of next generation sequencing (NGS) has fostered a shift in basic analytic strategies of a gene expression analysis in diverse pathologies for the purposes of research, pharmacology, and personalized medicine. What was once highly focused research on individual signaling pathways or pathway members has, from the time of gene expression arrays, become a global analysis of gene expression that has aided in identifying novel pathway interactions, the discovery of new therapeutic targets, and the establishment of disease-associated profiles for assessing progression, stratification, or a therapeutic response. But there are significant caveats to this analysis that do not allow for the construction of the full picture. The lack of timely updates to publicly available databases and the “hit and miss” deposition of scientific data to these databases relegate a large amount of potentially important data to “garbage”, begging the question, “how much are we really missing?” This brief perspective aims to highlight some of the limitations that RNA binding/modifying proteins and RNA processing impose on our current usage of NGS technologies as relating to cancer and how not fully appreciating the limitations of current NGS technology may negatively affect therapeutic strategies in the long run.
Assessing the Impact of Health Education Intervention on Asthma Knowledge, Attitudes, and Practices: A Cross-Sectional Study in Erbil, Iraq
Authors: Maulood Karwan Bahram , Khan Mohammad , Syed Sulaiman Syed Azhar , Khan Amer Hayat
Published: June 2023

Asthma causes chronic coughing, wheezing, dyspnea, and chest pressure. This study assessed asthmatic patients’ knowledge, attitudes, and practice (KAP) of bronchial asthma and proper education on its meaning, risk factors, symptoms, diagnosis, management, and prevention practices. We performed a cross-sectional interventional asthma KAP survey in Erbil, Iraq. We adapted a validated study questionnaire from KAP studies in other nations to the Erbil situation and culture. In Erbil, Kurdistan, Iraq, two major hospitals’ asthma clinics were studied. We chose 250 asthmatic patients from October 2018 to July 2019. Health education was comprehensive. The health education program used a Kurdish PowerPoint with a printout. Twenty-five groups received two weeks of one-hour health education pre-intervention. Each group was questioned before, 2 weeks after, and 12 weeks after health education. All data were analyzed by SPSS v26. The mean age of the respondents was 37.52 ± 15.16, with 48.7% of the respondents having a positive family history of asthma. After 2 weeks of health education intervention, respondents had a higher knowledge score and positive attitudes compared to pre-education, and after 12 weeks of education, there was a significant difference (p < 0.001) with improvised prevention practice. Health education programs led to considerable improvements in asthmatic patients’ knowledge, attitudes, and practices regarding their condition. After receiving health education for a period of two weeks, the majority of the participants answered correctly regarding asthma, its causes, and the elements that trigger asthma attacks.
Neurosensory Affectation in Patients Affected by Wolfram Syndrome: Descriptive and Longitudinal Analysis
Authors: Esteban-Bueno Gema , Berenguel Hernández Aída María , Fernández Fernández Nicolás , Navarro Cabrero Miguel , Coca Juan R.
Published: June 2023

Wolfram syndrome (WS) is a rare neurodegenerative disease usually of autosomal recessive origin. There is limited research about sensorineural hearing loss, despite it being a fundamental entity. It is important to broaden the study of this disease and specify a set of tests required for an adequate assessment of patients for efficient monitoring and control. The fundamental objective of this research is to understand WS from a biomedical perspective in order to help in its diagnosis, follow-up, and control. Pure tones audiometry, tympanometry, speech perception, the speech intelligibility index without aid, and testing at high frequencies were among the audiological measurements utilised since they were deemed suitable for standardised follow-up. Mixed linear models were used to examine the effects of age, time, or mean interaction in pure-tone (IPT), the average of high frequencies (HFA), auditory brainstem response (ABR), and brainstem auditory evoked potentials (BAEP). The genetic analysis allowed mutations to be classified into three phenotype-genotype groups, where the phenotype indicated the severity of the hearing loss. Patients with homozygous gene changes had a more severe neurosensory phenotype. The early discovery of sensorineural hearing loss and WS is crucial since it allows intensive follow-up and treatment of the person affected from the start.
Digital Health Innovations in the Battle against COVID-19: A Global Perspective
Authors: Koumpouros Yiannis
Published: June 2023

The COVID-19 pandemic has unleashed unprecedented challenges upon the global population, demanding rapid and innovative solutions [...]
Hemodynamic Consequence of Interventional Cardiac Catheterization in the Early Postoperative Period after Congenital Heart Surgery
Authors: Eason Daniel E. , Rossi Anthony F. , Aldawsari Khalifah A. , Patel Bhavi , Farooq Habiba , Khan Danyal M.
Published: June 2023

While still considered a high-risk procedure, cardiac catheterization during the early postoperative period is being performed more frequently in the current era. Limited data are currently available concerning the acute hemodynamic consequences of these procedures. Therefore, the purpose of this study was to evaluate the safety/efficacy of cardiac catheterization performed within thirty days of congenital heart surgery. We completed a retrospective review of all catheterizations within 30 days of congenital heart surgery. Procedures were performed due to failure to progress or hemodynamic deterioration. There were 1873 congenital heart surgeries during the study period. One hundred and three (6.2%) patients with a median age of 124 days underwent catheterization. Sixty-three cases received interventions, and forty patients underwent diagnostic catheterization. Early cardiac catheterization did not show a significant immediate change in the hemodynamics or inotrope score. Survival for patients undergoing diagnostic Cath (81%) did not differ significantly from the intervention group (89%). Although cardiac catheterization was performed on patients at the highest risk for death in the postoperative period, catheter intervention did not increase the risk of death. Those patients undergoing catheter intervention did not seem to experience major adverse events but achieved mild improvement in tissue perfusion.
Platelet Delta (δ)-Storage Pool Deficiency: A Case Series and Review of the Literature
Authors: Beirat Amir F. , Menakuru Sasmith R. , Kalra Maitri
Published: June 2023

Hereditary platelet delta (δ)-storage pool deficiency is a rare condition in which there are fewer dense granules in platelets disrupting primary hemostasis. It can cause a mild–moderate bleeding tendency with normal coagulation studies; hence, it is an underdiagnosed diagnostic challenge. The authors present three patients with hereditary platelet delta (δ)-storage pool deficiency who had heavy menstrual bleeding, excessive bleeding following surgery, mucocutaneous bleeding, and a bleeding score greater than or equal to 6. These cases reveal the susceptibility of underdiagnosing platelet disorders and the significance of utilizing a bleeding assessment tool to help guide further workup with transmission electron microscopy to visualize the fewer dense granules in platelets. Although bleeding is typically moderate, it can be severe in certain scenarios, like after mucosal surgeries, and can lead to death, highlighting the importance of the condition’s recognition and prophylactic treatment.
Designing a Health Strategy at Local Level: A Conceptual Framework for Local Governments
Authors: Santinha Gonçalo , Fernandes Alexandre , Oliveira Rafaela , Rocha Nelson Pacheco
Published: June 2023

This article addresses a gap in the literature regarding the design and implementation of health and well-being strategies by local governments. It presents a conceptual framework that can help local governments to include health and well-being among their political priorities by promoting the participation of local stakeholders and the community in general. The article also highlights the important role that local governments play in public health, especially in times of crisis, such as the COVID-19 pandemic. To demonstrate the approach, the manuscript explores the recent shift toward decentralization in Portugal and the resulting emphasis on local governments leading new approaches to health governance. The planning process for Arouca’s Health Strategy, an inland municipality, is used as a case study to demonstrate the approach, which emphasizes the meaningful participation of the entire local community. The article concludes by noting that there is still significant room for improvement in all areas related to health and well-being and a need to adopt new multistakeholder governance arrangements to sustain the institutional capacity for upcoming strategies.
“It Happened to Me and It’s Serious”: Conditional Indirect Effects of Infection Severity Narrated in Testimonial Tweets on COVID-19 Prevention
Authors: Igartua Juan-José , Rodríguez-Contreras Laura , Guerrero-Martín Íñigo , Honorato-Vicente Andrea
Published: June 2023

The health crisis caused by COVID-19 resulted in societal breakdowns around the world. Our research is based on determining which features of testimonial messages are most relevant in increasing persuasive impact. An online experiment with a 2 (severity infection narrative: low vs. high) × 2 (infection target: narrative’s protagonist vs. protagonist’s father) between-subject factorial design was carried out. Young people between 18 and 28 years (N = 278) were randomly assigned to one of the four experimental conditions, where they were asked to read a narrative message in the form of a Twitter thread describing a COVID-19 infection (with mild or severe symptoms) that affected either the protagonist of the message (a 23-year-old young person) or their father. After reading the narrative message, the mediating and dependent variables were evaluated. A message describing a severe COVID-19 infection affecting their protagonist to increase the perception of personal risk increased the persuasive impact through an increase in cognitive elaboration and a reduction in reactance. Our study highlights that creating persuasive messages based on social media targeted at young people that describe a careless behavior resulting in a severe COVID-19 infection can be an appropriate strategy for designing prevention campaigns.
Integration of Transcriptomic and Metabolomic Profiles Provides Insights into the Influence of Nitrogen on Secondary Metabolism in Fusarium sacchari
Authors: Bao Yixue , Lin Zhenyue , Yao Wei , Akbar Sehrish , Lin Wenfeng , Powell Charles A. , Xu Jianlong , Zhang Muqing
Published: June 2023

Nitrogen availability might play an essential role in plant diseases by enhancing fungal cell growth and influencing the expression of genes required for successful pathogenesis. Nitrogen availability could modulate secondary metabolic pathways as evidenced by the significant differential expression of several core genes involved in mycotoxin biosynthesis and genes encoding polyketide synthase/nonribosomal peptide synthetases, cytochrome P450 and carbohydrate-active enzymes in Fusarium sacchari, grown on different nitrogen sources. A combined analysis was carried out on the transcript and metabolite profiles of regulatory metabolic processes and the virulence of Fusarium sacchari grown on various nitrogen sources. The nitrogen regulation of the gibberellin gene cluster included the metabolic flux and multiple steps of gibberellin synthesis. UHPLC-MS/MS-based metabolome analysis revealed the coordination of these related transcripts and the accumulation of gibberellin metabolites. This integrated analysis allowed us to uncover additional information for a more comprehensive understanding of biological events relevant to fungal secondary metabolic regulation in response to nitrogen availability.
Accumulation of Renal Fibrosis in Hyperuricemia Rats Is Attributed to the Recruitment of Mast Cells, Activation of the TGF-β1/Smad2/3 Pathway, and Aggravation of Oxidative Stress
Authors: Zhang Mingkang , Cui Ruirui , Zhou Yan , Ma Yanrong , Jin Yongwen , Wang Lina , Kou Wen , Wu Xin’an
Published: June 2023

Renal fibrosis is relentlessly progressive and irreversible, and a life-threatening risk. With the continuous intake of a high-purine diet, hyperuricemia has become a health risk factor in addition to hyperglycemia, hypertension, and hyperlipidemia. Hyperuricemia is also an independent risk factor for renal interstitial fibrosis. Numerous studies have reported that increased mast cells (MCs) are closely associated with kidney injury induced by different triggering factors. This study investigated the effect of MCs on renal injury in rats caused by hyperuricemia and the relationship between MCs and renal fibrosis. Our results reveal that hyperuricemia contributes to renal injury, with a significant increase in renal MCs, leading to renal fibrosis, mitochondrial structural disorders, and oxidative stress damage. The administration of the MCs membrane stabilizer, sodium cromoglycate (SCG), decreased the expression of SCF/c-kit, reduced the expression of α-SMA, MMP2, and inhibited the TGF-β1/Smad2/3 pathway, thereby alleviating renal fibrosis. Additionally, SCG reduced renal oxidative stress and mitigated mitochondrial structural damage by inhibiting Ang II production and increasing renal GSH, GSH-Px, and GR levels. Collectively, the recruitment of MCs, activation of the TGF-β1/Smad2/3 pathway, and Ang II production drive renal oxidative stress, ultimately promoting the progression of renal fibrosis in hyperuricemic rats.
Bortezomib Increased Vascular Permeability by Decreasing Cell–Cell Junction Molecules in Human Pulmonary Microvascular Endothelial Cells
Authors: Matsumoto Taichi , Matsumoto Junichi , Matsushita Yuka , Arimura Moeno , Aono Kentaro , Aoki Mikiko , Terada Kazuki , Mori Masayoshi , Haramaki Yutaka , Imatoh Takuya , Yamauchi Atsushi , Migita Keisuke
Published: June 2023

Bortezomib (BTZ), a chemotherapeutic drug used to treat multiple myeloma, induces life-threatening side effects, including severe pulmonary toxicity. However, the mechanisms underlying these effects remain unclear. The objectives of this study were to (1) investigate whether BTZ influences vascular permeability and (2) clarify the effect of BTZ on the expression of molecules associated with cell–cell junctions using human pulmonary microvascular endothelial cells in vitro. Clinically relevant concentrations of BTZ induced limited cytotoxicity and increased the permeability of human pulmonary microvascular endothelial cell monolayers. BTZ decreased the protein expression of claudin-5, occludin, and VE-cadherin but not that of ZO-1 and β-catenin. Additionally, BTZ decreased the mRNA expression of claudin-5, occludin, ZO-1, VE-cadherin, and β-catenin. Our results suggest that BTZ increases the vascular permeability of the pulmonary microvascular endothelium by downregulating cell–cell junction molecules, particularly claudin-5, occludin, and VE-cadherin.
Mining the Australian Grains Gene Bank for Rust Resistance in Barley
Authors: Arifuzzaman Md , Jost Matthias , Wang Meinan , Chen Xianming , Perovic Dragan , Park Robert F. , Rouse Matthew , Forrest Kerrie , Hayden Matthew , Khan Ghazanfar Abbas , Dracatos Peter M.
Published: June 2023

Global barley production is threatened by plant pathogens, especially the rusts. In this study we used a targeted genotype-by-sequencing (GBS) assisted GWAS approach to identify rust resistance alleles in a collection of 287 genetically distinct diverse barley landraces and historical cultivars available in the Australian Grains Genebank (AGG) and originally sourced from Eastern Europe. The accessions were challenged with seven US-derived cereal rust pathogen races including Puccinia hordei (Ph-leaf rust) race 17VA12C, P. coronata var. hordei (Pch-crown rust) race 91NE9305 and five pathogenically diverse races of P. striiformis f. sp. hordei (Psh-stripe rust) (PSH-33, PSH-48, PSH-54, PSH-72 and PSH-100) and phenotyped quantitatively at the seedling stage. Novel resistance factors were identified on chromosomes 1H, 2H, 4H and 5H in response to Pch, whereas a race-specific QTL on 7HS was identified that was effective only to Psh isolates PSH-72 and PSH-100. A major effect QTL on chromosome 5HL conferred resistance to all Psh races including PSH-72, which is virulent on all 12 stripe rust differential tester lines. The same major effect QTL was also identified in response to leaf rust (17VA12C) suggesting this locus contains several pathogen specific rust resistance genes or the same gene is responsible for both leaf rust and stripe rust resistance. Twelve accessions were highly resistant to both leaf and stripe rust diseases and also carried the 5HL QTL. We subsequently surveyed the physical region at the 5HL locus for across the barley pan genome variation in the presence of known resistance gene candidates and identified a rich source of high confidence protein kinase and antifungal genes in the QTL region.
Quantum Entanglement and State-Transference in Fenna–Matthews–Olson Complexes: A Post-Experimental Simulation Analysis in the Computational Biology Domain
Authors: Delgado Francisco , Enríquez Marco
Published: June 2023

Fenna-Mathews-Olson complexes participate in the photosynthetic process of Sulfur Green Bacteria. These biological subsystems exhibit quantum features which possibly are responsible for their high efficiency; the latter may comprise multipartite entanglement and the apparent tunnelling of the initial quantum state. At first, to study these aspects, a multidisciplinary approach including experimental biology, spectroscopy, physics, and math modelling is required. Then, a global computer modelling analysis is achieved in the computational biology domain. The current work implements the Hierarchical Equations of Motion to numerically solve the open quantum system problem regarding this complex. The time-evolved states obtained with this method are then analysed under several measures of entanglement, some of them already proposed in the literature. However, for the first time, the maximum overlap with respect to the closest separable state is employed. This authentic multipartite entanglement measure provides information on the correlations, not only based on the system bipartitions as in the usual analysis. Our study has led us to note a different view of FMO multipartite entanglement as tiny contributions to the global entanglement suggested by other more basic measurements. Additionally, in another related trend, the initial state, considered as a Förster Resonance Energy Transfer, is tracked using a novel approach, considering how it could be followed under the fidelity measure on all possible permutations of the FMO subsystems through its dynamical evolution by observing the tunnelling in the most probable locations. Both analyses demanded significant computational work, making for a clear example of the complexity required in computational biology.
The Slow Progression of Diabetic Retinopathy Is Associated with Transient Protection of Retinal Vessels from Death
Authors: Li Yanliang , Baccouche Basma , Del-Risco Norma , Park Jason , Song Amy , McAnany J. Jason , Kazlauskas Andrius
Published: June 2023

The purpose of this study was to investigate the reason that diabetic retinopathy (DR) is delayed from the onset of diabetes (DM) in diabetic mice. To this end, we tested the hypothesis that the deleterious effects of DM are initially tolerated because endogenous antioxidative defense is elevated and thereby confers resistance to oxidative stress-induced death. We found that this was indeed the case in both type 1 DM (T1D) and type 2 DM (T2D) mouse models. The retinal expression of antioxidant defense genes was increased soon after the onset of DM. In addition, ischemia/oxidative stress caused less death in the retinal vasculature of DM versus non-DM mice. Further investigation with T1D mice revealed that protection was transient; it waned as the duration of DM was prolonged. Finally, a loss of protection was associated with the manifestation of both neural and vascular abnormalities that are diagnostic of DR in mice. These observations demonstrate that DM can transiently activate protection from oxidative stress, which is a plausible explanation for the delay in the development of DR from the onset of DM.