bluuebunny/crossref_metadata_2025 huggingface dataset:
Low Release Study of Cefotaxime by Functionalized Mesoporous Silica Nanomaterials
Authors: Mihaiescu Dan Eduard , Istrati Daniela , Moroșan Alina , Stanca Maria , Purcăreanu Bogdan , Cristescu Rodica , Vasile Bogdan Ștefan , Trușca Roxana Doina
Published: November 2022

As a third-generation β-lactam antibiotic, cefotaxime shows a broad-spectrum with Gram-positive and Gram-negative bacteria activity and is included in WHO’s essential drug list. In order to obtain new materials with sustained release properties, the present research focuses on the study of cefotaxime absorption and desorption from different functionalized mesoporous silica supports. The MCM-41-type nanostructured mesoporous silica support was synthesized by sol–gel technique using a tetraethyl orthosilicate (TEOS) route and cetyltrimethylammonium bromide (CTAB) as a surfactant, at room temperature and normal pressure. The obtained mesoporous material (MCM-41 class) was characterized through nuclear magnetic resonance (NMR), scanning electron microscopy (SEM), high-resolution transmission electron microscopy (HR-TEM), N2 absorption–desorption (BET) and Fourier transform infrared spectroscopy (FT-IR), proving a good micro-structured homogeneity (SEM images), a high surface area (BET, 1029 m2/g) correlated with high silanolic activity (Q3/Q4 peak ratio from 29Si MAS-NMR), and an expected uniform hexagonal structure (2–3 nm, HRTEM). In order to non-destructively link the antibiotic compound on the solid phase, MCM-41 was further functionalized in two steps: with aminopropyl trimethoxysilane (APTMS) and glutaraldehyde (GA). Three cefotaxime-loaded materials were comparatively studied for low release capacity: the reference material with adsorbed cefotaxime on MCM-41, MCM-41/APS (aminopropyl silyl surface functionalization) adsorbed cefotaxime material, and APTMS–GA bounded MCM-41—cefotaxime material. The slow-release profiles were obtained by using an on-flow modified HPLC system. A significant improved release capacity was identified in the case of MCM-41/APS/GA—cefotaxime due to the covalent surface grafting of the biological active compound, recommending this class of materials as an effective carrier of bioactive compounds in wound dressing, anti-biofilm coatings, advanced drugs, and other related applications.
Effects of Hydroxypropyl and Lactate Esterified Glutinous Rice Starch on Wheat Starch Gel Construction
Authors: Gong Yongqiang , Gu Tingting , Zhang Tiantian , Li Songnan , Yu Zhenyu , Zheng Mingming , Xiao Yaqing , Zhou Yibin
Published: November 2022

An investigation was conducted into the impacts of hydroxypropyl glutinous rice starch (HPGRS) and lactate-esterified glutinous rice starch (LAEGRS) on a dilute solution and gel properties of wheat starch (WS) at different proportions (0%, 1%, 3%, 5%, and 10%). The results of dilute solution viscosity showed that hydroxypropyl treatment of glutinous rice starch (GRS) could promote the extension of GRS chains, while lactate esterification led to the hydrophobic association of GRS chains, and the starch chains curled inward. Different HPGRS: WS and LAEGRS: WS ratios, β > 0 and ∆b > 0, showed HPGRS and LAEGRS produced attractive forces with WS and formed a uniform gel structure. Compared with WS gel, HPGRS, and LAEGRS could effectively delay the short-term aging of WS gels, and LAEGRS had a more significant effect. HPGRS increased the pasting viscosity, viscoelasticity, and springiness of WS gels, reduced the free water content, and established a tighter gel network structure, while LAEGRS had an opposite trend on WS. In conclusion, HPGRS was suitable for WS-based foods with stable gel network structure and high water retention requirements, and LAEGRS was suitable for WS-based foods with low viscosity and loose gel structure.
Balancing at the Borderline of a Breed: A Case Study of the Hungarian Short-Haired Vizsla Dog Breed, Definition of the Breed Profile Using Simple SNP-Based Methods
Authors: Varga László , Edviné Erika Meleg , Hudák Péter , Anton István , Pálinkás-Bodzsár Nóra , Zsolnai Attila
Published: November 2022

The aim of this study was to determine the breed boundary of the Hungarian Short-haired Vizsla (HSV) dog breed. Seventy registered purebred HSV dogs were genotyped on approximately 145,000 SNPs. Principal Component Analysis (PCA) and Admixture analysis certified that they belong to the same population. The outer point of the breed demarcation was a single Hungarian Wire-haired Vizsla (HWV) individual, which was the closest animal genetically to the HSV population in the PCA analysis. Three programs were used for the breed assignment calculations, including the widely used GeneClass2.0 software and two additional approaches developed here: the ‘PCA-distance’ and ‘IBS-central’ methods. Both new methods calculate a single number that represents how closely a dog fits into the actual reference population. The former approach calculates this number based on the PCA distances from the median of HSV animals. The latter calculates it from identity by state (IBS) data, measuring the distance from a central animal that is the best representative of the breed. Having no mixed-breed dogs with known HSV genome proportion, admixture animals were simulated by using data of HSV and HWV individuals to calibrate the inclusion/exclusion probabilities for the assignment. The numbers generated from these relatively simple calculations can be used by breeders and clubs to keep their populations under genetic supervision.
Multi-Omic Profiles in Infants at Risk for Food Reactions
Authors: Beheshti Ramin , Stone Shane , Chandran Desirae , Hicks Steven D.
Published: November 2022

Food reactions (FR) are multifactorial and impacted by medical, demographic, environmental, and immunologic factors. We hypothesized that multi-omic analyses of host-microbial factors in saliva would enhance our understanding of FR development. This longitudinal cohort study included 164 infants followed from birth through two years. The infants were identified as FR (n = 34) or non-FR (n = 130) using the Infant Feeding Practice II survey and medical record confirmation. Saliva was collected at six months for the multi-omic assessment of cytokines, mRNAs, microRNAs, and the microbiome/virome. The levels of one miRNA (miR-203b-3p, adj. p = 0.043, V = 2913) and one viral phage (Proteus virus PM135, adj. p = 0.027, V = 2955) were lower among infants that developed FRs. The levels of one bacterial phylum (Cyanobacteria, adj. p = 0.048, V = 1515) were higher among infants that developed FR. Logistical regression models revealed that the addition of multi-omic features (miR-203b-3p, Cyanobacteria, and Proteus virus PM135) improved predictiveness for future FRs in infants (p = 0.005, X2 = 12.9), predicting FRs with 72% accuracy (AUC = 0.81, sensitivity = 72%, specificity = 72%). The multi-omic analysis of saliva may enhance the accurate identification of infants at risk of FRs and provide insights into the host/microbiome interactions that predispose certain infants to FRs.
Complete Chloroplast Genome Sequence of the Endemic and Endangered Plant Dendropanax oligodontus: Genome Structure, Comparative and Phylogenetic Analysis
Authors: Wang Yong , Yu Jing , Chen Yu-Kai , Wang Zhu-Cheng
Published: November 2022

Dendropanax oligodontus, which belongs to the family Araliaceae, is an endemic and endangered species of Hainan Island, China. It has potential economic and medicinal value owing to the presence of phenylpropanoids, flavonoids, triterpenoids, etc. The analysis of the structure and characteristics of the D. oligodontus chloroplast genome (cpDNA) is crucial for understanding the genetic and phylogenetic evolution of this species. In this study, the cpDNA of D. oligodontus was sequenced for the first time using next-generation sequencing methods, assembled, and annotated. We observed a circular quadripartite structure comprising a large single-copy region (86,440 bp), a small single-copy region (18,075 bp), and a pair of inverted repeat regions (25,944 bp). The total length of the cpDNA was 156,403 bp, and the GC% was 37.99%. We found that the D. oligodontus chloroplast genome comprised 131 genes, with 86 protein-coding genes, 8 rRNA genes, and 37 tRNAs. Furthermore, we identified 26,514 codons, 13 repetitive sequences, and 43 simple sequence repeat sites in the D. oligodontus cpDNA. The most common amino acid encoded was leucine, with a strong A/T preference at the third position of the codon. The prediction of RNA editing sites in the protein-coding genes indicated that RNA editing was observed in 19 genes with a total of 54 editing sites, all of which involved C-to-T transitions. Finally, the cpDNA of 11 species of the family Araliaceae were selected for comparative analysis. The sequences of the untranslated regions and coding regions among 11 species were highly conserved, and minor differences were observed in the length of the inverted repeat regions; therefore, the cpDNAs were relatively stable and consistent among these 11 species. The variable hotspots in the genome included clpP, ycf1, rnK-rps16, rps16-trnQ, atpH-atpI, trnE-trnT, psbM-trnD, ycf3-trnS, and rpl32-trnL, providing valuable molecular markers for species authentication and regions for inferring phylogenetic relationships among them, as well as for evolutionary studies. Evolutionary selection pressure analysis indicated that the atpF gene was strongly subjected to positive environmental selection. Phylogenetic analysis indicated that D. oligodontus and Dendropanax dentiger were the most closely related species within the genus, and D. oligodontus was closely related to the genera Kalopanax and Metapanax in the Araliaceae family. Overall, the cp genomes reported in this study will provide resources for studying the genetic diversity and conservation of the endangered plant D. oligodontus, as well as resolving phylogenetic relationships within the family.
CRISPR/Cas9 Genome-Editing Technology and Potential Clinical Application in Gastric Cancer
Authors: Almeida Renata Sanches , Wisnieski Fernanda , Takao Real Karia Bruno , Smith Marilia Arruda Cardoso
Published: November 2022

Gastric cancer is the subject of clinical and basic studies due to its high incidence and mortality rates worldwide. Due to the diagnosis occurring in advanced stages and the classic treatment methodologies such as gastrectomy and chemotherapy, they are extremely aggressive and limit the quality of life of these patients. CRISPR/Cas9 is a tool that allows gene editing and has been used to explore the functions of genes related to gastric cancer, in addition to being used in the treatment of this neoplasm, greatly increasing our understanding of cancer genomics. In this mini-review, we seek the current status of the CRISPR/Cas9 gene-editing technology in gastric cancer research and clinical research.
Primary Healthcare Nurse’s Barriers and Facilitators to Providing Sexual and Reproductive Healthcare Services of LGBTQI Individuals: A Qualitative Study
Authors: Seretlo Raikane James , Mokgatle Mathildah Mpata
Published: November 2022

In most cases, we only hear Lesbians, Gays, Bisexuals, Transgender, Queer, and Intersex (LGBTQI) patients complaining about nurses being the reason for not accessing and utilizing healthcare services; for example, studies reports on the different attitudes of healthcare providers including nurses against LGBTQI patients. However, factors influencing the behavior of South African Primary Healthcare (PHC) Nurses toward LGBTQI patients are rarely reported. The study aimed to explore how PHC nurses experienced and perceived sexual and reproductive health services for LGBTQI individuals in Tshwane, Gauteng Province, South Africa. The study followed qualitative research using an exploratory design approach. The sample included 27 PHC nurses from Tshwane, Gauteng Province, South Africa. In-depth face-to-face interviews were coded and analyzed using Thematic Content Analysis (TCA) which included five interrelated steps. The results revealed three main themes: barriers to the provision of LGBTQI-related SRHS, facilitators for the provision of SRHS to LGBTQI individuals, and strategies to improve LGBTQI individuals’ SRHS accessibility and availability. Common barriers were related to the institutions, PHC nurses, the general public, and LGBTQI patients themselves. Regardless of the challenges faced by PHC nurses, there were some enabling factors that pushed them to continue rendering SHRS to LGBTQI patients who came to their clinics. Almost all PHC nurses suggested the importance of awareness, transparency, collaboration, and the need for training related to LGBTQI healthcare issues.
Molecular Characterization and Antibiogram of Acinetobacter baumannii Clinical Isolates Recovered from the Patients with Ventilator-Associated Pneumonia
Authors: Saleem Mohd , Syed Khaja Azharuddin Sajid , Hossain Ashfaque , Alenazi Fahaad , Said Kamaleldin B. , Moursi Soha Abdallah , Almalaq Homoud Abdulmohsin , Mohamed Hamza , Rakha Ehab
Published: November 2022

A 2-year prospective study carried out on ventilator-associated pneumonia (VAP) patients in the intensive care unit at a tertiary care hospital, Hail, Kingdom of Saudi Arabia (KSA), revealed a high prevalence of extremely drug-resistant (XDR) Acinetobacter baumannii. About a 9% increase in the incidence rate of A. baumannii occurred in the VAP patients between 2019 and 2020 (21.4% to 30.7%). In 2019, the isolates were positive for IMP-1 and VIM-2 (31.1% and 25.7%, respectively) as detected by PCR. In comparison, a higher proportion of isolates produced NDM-1 in 2020. Here, we observed a high proportion of resistant ICU isolates towards the most common antibiotics in use. Colistin sensitivity dropped to 91.4% in the year 2020 as compared to 2019 (100%). Thus, the finding of this study has a highly significant clinical implementation in the clinical management strategies for VAP patients. Furthermore, strict implementation of antibiotic stewardship policies, regular surveillance programs for antimicrobial resistance monitoring, and screening for genes encoding drug resistance phenotypes have become imperative.
Quality of Life and Depressive Symptoms in Transcatheter Aortic Valve Implementation Patients—A Cross-Sectional Study
Authors: Olszewska-Turek Katarzyna , Bętkowska-Korpała Barbara
Published: November 2022

Quality of life is an important factor influencing mood. In any group of elderly people undergoing valve implementation or surgical aortic valve replacement, one in three will have depressive symptoms. The aims of this study were as follows: 1. to evaluate the impact of health-related quality of life on depressive symptoms in elderly patients undergoing TAVI, and 2. to analyze beliefs about TAVI. Methods: A total of 131 elderly people (mean age: 82.1 ± 6.1 years) scheduled to receive TAVI completed the Geriatric Depression Scale, EQ-5D-3L, and Mini Mental State Examination. A total of 43 patients completed the questionnaires after the treatment. The narrative interview analyses were performed based on 20 randomly selected patients after TAVI. Results: The mean level of general depression before TAVI was 4.19 ± 2.83, and after it was 3.12 ± 2.52 (p = 0.02), and the frequency decreased from 20% to 3%. An increase in the level of activity and number of interests and a decrease in life satisfaction were identified. The higher the general quality of life was, the lower the levels of depressiveness before and after TAVI were (r = 0.26 vs. r = 0.48; p < 0.05). Conclusions: Patients differed in their depressive symptoms, as well as their intensity and frequency, before and after TAVI. These results underscore the importance of screening for depression at baseline and reassessing changes in depressiveness during follow-up.
The Impacts of the Emerging Climate Change on Broccoli (Brassica oleracea L. var. italica Plenck.) Crop
Authors: Siomos Anastasios S. , Koularmanis Konstantinos , Tsouvaltzis Pavlos
Published: November 2022

Broccoli (Brassica oleracea L. var. italica Plenck.) is nowadays one of the most important vegetable crops worldwide, with an increasing demand by the market, due to its high nutritional value. Based on the optimal temperature range, its cultivation in the temperate regions takes place from late summer to late spring. Broccoli cultivation nowadays faces many challenges, such as the profitable production throughout the year, as well as during conditions of an increased temperature, induced by the emerging climate change, as well as the possibility of introducing the cultivation to subtropical and tropical areas. The modern genotypes (F1 hybrids) differ significantly among themselves in terms of the requirements for the head’s formation, which, however, has not been fully elucidated. In this direction, breeders have been developing programs internationally since the early 1990s, in order to create genotypes that will be adapted to temperatures higher than the optimal range, having, however, a relatively limited initial germplasm pool. The purpose of this review is to present extensively the temperature requirements in the broccoli crop production and to highlight the impacts of the emerging climate change.
nZVI Mobility and Transport: Laboratory Test and Numerical Model
Authors: Viotti Paolo , Sappa Giuseppe , Tatti Fabio , Andrei Francesca
Published: November 2022

Zerovalent iron nanoparticles (nZVI) are becoming one of the most widely recommended nanomaterials for soil and groundwater remediation. However, when nZVI are injected in the groundwater flow, the behavior (mobility, dispersion, distribution) is practically unknown. This fact generally results in the use of enormous quantities of them at the field scale. The uncertainties are on the effective volumes reached from the plume of nZVI because their tendency to aggregate and their weight can cause their settling and deposition. So, the mobility of nanoparticles is a real issue, which can often lead to inefficient or expensive soil remediation. Furthermore, there is another aspect that must be considered: the fate of these nZVI in the groundwater and their possible impact on the subsoil environment. All these considerations have led us to propose an application of nZVI simulating the permeation technique through a laboratory experience, finalized to have a better, or even simpler description of their real behavior when injected in a flow in the subsoil. A two-dimensional laboratory-scale tank was used to study the dispersion and transport of nZVI. A nZVI solution, with a concentration equal to 4.54 g/L, was injected into glass beads, utilized as porous medium. The laboratory experiment included a digital camera to acquire the images. The images were then used for calibrating a numerical model. The results of the mass balance confirm the validity of the proposed numerical model, obtaining values of velocity (5.41 × 10−3 m/s) and mass (1.9 g) of the nZVI of the same order of those from the experimental tests. Several information were inferred from both experimental and numerical tests. Both demonstrate that nZVI plume does not behave as a solute dissolved in water, but as a mass showing its own mobility ruled mainly from the buoyancy force. A simple simulation of a tracer input and a nZVI plume are compared to evidence the large differences between their evolution in time and space. This means that commercial numerical models, if not corrected, cannot furnish a real forecast of the volume of influence of the injected nZVI. Further deductions can be found from the images and confirmed by means the numerical model where the detachment effect is much smaller than the attachment one (ratio kd/ka = 0.001). From what is reported, it is worthwhile to pay attention on the localization of the contaminants source/plume to reach an effective treatment and it is important to go further in the improvement of solution for the limiting the nanoparticles aggregation phenomenon.
Infant Death Clustering in the Quarter of a Century in India: A Decomposition Analysis
Authors: Ranjan Mukesh , Dwivedi Laxmi Kant , Halli Shivalingappa
Published: November 2022

The study aims to examine the clustering of infant deaths in India and the relative contribution of infant death clustering after accounting for the socio-economic and biodemographic factors that explain the decline in infant deaths. The study utilized 10 years of birth history data from three rounds of the National Family Health Survey (NFHS). The random effects dynamic probit model was used to decompose the decline in infant deaths into the contributions by the socio-economic and demographic factors, including the lagged independent variable, the previous infant death measuring the clustering of infant deaths in families. The study found that there has been a decline in the clustering of infant deaths among families during the past two and half decades. The simulation result shows that if the clustering of infant deaths in families in India was completely removed, there would be a decline of nearly 30 percent in the infant mortality rate (IMR). A decomposition analysis based on the dynamic probit model shows that for NFHS-1 and NFHS-3, in the total change of the probability of infant deaths, the rate of change for a given population composition contributed around 45 percent, and about 44 percent was explained by a compositional shift. Between NFHS-3 and NFHS-4, the rate of change for a given population composition contributed 86%, and the population composition for a given rate contributed 10% to the total change in the probability of infant deaths. Within this rate, the contribution of a previous infant was 0.8% and the mother’s age was 10%; nearly 31% was contributed by the region of residence, 69% by the mother’s education, and around 20% was contributed by the wealth index and around 8.7% by the sex of the child. The mother’s unobserved factors contributed more than 50 percent to the variability of infant deaths in all the survey rounds and was also statistically significant (p < 0.01). Bivariate analysis suggests that women with two or more infant losses were much less likely to have full immunization (10%) than women with no infant loss (62%), although institutional delivery was high among both groups of women.
Occurrences of Workplace Violence Related to the COVID-19 Pandemic, United States, March 2020 to August 2021
Authors: Marsh Suzanne M. , Rocheleau Carissa M. , Carbone Eric G. , Hartley Daniel , Reichard Audrey A. , Tiesman Hope M.
Published: November 2022

As businesses dealt with an increasingly anxious public during the COVID-19 pandemic and were frequently tasked with enforcing various COVID-19 prevention policies such as mask mandates, workplace violence and harassment (WPV) emerged as an increasing important issue affecting worker safety and health. Publicly available media reports were searched for WPV events related to the COVID-19 pandemic that occurred during 1 March 2020, and 31 August 2021, using Google News aggregator services scans with data abstraction and verification. The search found 408 unique WPV events related to COVID-19. Almost two-thirds involved mask disputes. Over half (57%) of the 408 events occurred in retail (38%) and food service (19%). We also conducted a comparison of events identified in this search to a similar study of media reports between March 2020 to October 2020 that used multiple search engines to identify WPV events. Despite similar conclusions, a one-to-one comparison of relevant data from these studies found only modest overlap in the incidents identified, suggesting the need to make improvements to future efforts to extract data from media reports. Prevention resources such as training and education for workers may help industries de-escalate or prevent similar WPV events in the future.
Fatigue Increases Muscle Activations but Does Not Change Maximal Joint Angles during the Bar Dip
Authors: McKenzie Alec , Crowley-McHattan Zachary , Meir Rudi , Whitting John , Volschenk Wynand
Published: November 2022

The purpose of this study was to profile and compare the bar dip’s kinematics and muscle activation patterns in non-fatigued and fatigued conditions. Fifteen healthy males completed one set of bar dips to exhaustion. Upper limb and trunk kinematics, using 3D motion capture, and muscle activation intensities of nine muscles, using surface electromyography, were recorded. The average kinematics and muscle activations of repetitions 2–4 were considered the non-fatigued condition, and the average of the final three repetitions was considered the fatigued condition. Paired t-tests were used to compare kinematics and muscle activation between conditions. Fatigue caused a significant increase in repetition duration (p < 0.001) and shifted the bottom position to a significantly earlier percentage of the repetition (p < 0.001). There were no significant changes in the peak joint angles measured. However, there were significant changes in body position at the top of the movement. Fatigue also caused an increase in peak activation amplitude in two agonist muscles (pectoralis major [p < 0.001], triceps brachii [p < 0.001]), and three stabilizer muscles. For practitioners prescribing the bar dip, fatigue did not cause drastic alterations in movement technique and appears to target pectoralis major and triceps brachii effectively.
Literacy and Mental Health of Portuguese Higher Education Students and Their Use of Health Promotion Strategies during Confinement in the COVID-19 Pandemic
Authors: Oliveira Ana , Nobre Joana , Luis Henrique , Luis Luis , Albacar-Riobóo Núria , Pinho Lara , Sequeira Carlos
Published: November 2022

The pandemic of COVID-19 caused significant changes in economies and societies with a major impact on the entire education process. However, these changes did not invalidate a constant effort of adaptation. This cross-sectional, descriptive, and correlational study used an online questionnaire administered to students from various study cycles at higher education institutions in Portugal, with the aim of exploring the influence of literacy and mental health on the use of mental health promotion strategies during COVID-19 confinement. A total of 329 students from higher education institutions participated in this study, mostly from the age group 18–24 years (n = 272; 82.7%) and female (n = 265, 80.5%). The most mentioned health promotion strategies during this period included studying (n = 170; 51.7%); physical activities (n = 151, 45.9%); social networking (n = 124, 37.7%); cooking activities (n = 120, 36.5%); and listening to music (n = 118, 35.9%). Academic success is self-reported, and it is weakly correlated with the MHI5 (r = 0.103, p = 0.063). Students in the pre-graduate programs studied more during the times of the pandemic and used this activity as a mental-health-promoting strategy with a statistically significant difference (p = 0.033). Although it was difficult to improve health literacy related to COVID-19 in such a short period of time, there was a very strong motivation to access, understand, evaluate, communicate, synthesize, and apply information and knowledge to maintain mental health through self-care using health promotion strategies.
Effects of Social Support and Loneliness on the Irrational Consumption Tendencies of Healthcare Products among the Elderly: A Structural Equation Model
Authors: Chen Yating , Li Luqi , Tan Zhiji , Ma Chengcheng , Wang Binyan , Guo Qing , Li Li
Published: November 2022

Background: In recent years, with the development of the social economy and an improvement in health consciousness, the levels of demand and consumption for healthcare products have been increasing rapidly among the elderly. However, the irrational consumption of healthcare products has caused widespread concern, as it can generate economic losses and have negative effects on psychological health. Therefore, it is critical to identify the variables that can reduce tendencies toward irrational consumption in the area of healthcare products. This study aimed to explore the relationship between the irrational consumption tendencies of healthcare products, social support, and loneliness among elderly people in Hangzhou, China. Methods: In 2021, a cross-sectional survey involving 485 elderly people from three districts in Hangzhou, China, was conducted. Descriptive statistics were calculated for socioeconomic status and demographic characteristics, level of loneliness, social support, and irrational consumption tendencies. A structural equation model was used to test the impact of social support on the irrational consumption tendencies of healthcare products among the elderly and to explore the mediating effects of loneliness. Results: The findings reveal that the average levels of social support and loneliness among the elderly were 30.63 points (total = 44 points) and 6.88 points (15 points), respectively. The average scores of the four subscales of irrational consumption tendencies, which were named susceptibility to persuasion, interpersonal influences, pursuit of added value, and fears of aging were 2.48, 2.93, 2.48, and 3.17 points (5 points), respectively. Social support had a significant effect on irrational consumption tendencies, and loneliness played a partial mediating role between social support and irrational consumption tendencies. Conclusions: A relationship model was constructed to examine the association between loneliness, social support, and irrational consumption tendencies among older people in relation to healthcare products. From a social support and psychological perspective, advice and countermeasures are proposed to prevent the irrational consumption of healthcare products among older people.
Exploring the Impact of Localized COVID-19 Events on Intercity Mobility during the Normalized Prevention and Control Period in China
Authors: Xie Mingke , Chen Yang , Tang Luliang
Published: November 2022

Uncontrolled, large-scale human mobility can amplify a localized disease into a pandemic. Tracking changes in human travel behavior, exploring the relationship between epidemic events and intercity travel generation and attraction under policies will contribute to epidemic prevention efforts, as well as deepen understanding of the essential changes of intercity interactions in the post-epidemic era. To explore the dynamic impact of small-scale localized epidemic events and related policies on intercity travel, a spatial lag model and improved gravity models are developed by using intercity travel data. Taking the localized COVID-19 epidemic in Xi’an, China as an example, the study constructs the travel interaction characterization before or after the pandemic as well as under constraints of regular epidemic prevention policies, whereby significant impacts of epidemic events are explored. Moreover, indexes of the quantified policies are refined to the city level in China to analyze their effects on travel volumes. We highlight the non-negligible impacts of city events and related policies on intercity interaction, which can serve as a reference for travel management in case of such severe events.
Nitrate in Maternal Drinking Water during Pregnancy and Measures of Male Fecundity in Adult Sons
Authors: Clemmensen Pernille Jul , Brix Nis , Schullehner Jörg , Gaml-Sørensen Anne , Toft Gunnar , Tøttenborg Sandra Søgaard , Ebdrup Ninna Hinchely , Hougaard Karin Sørig , Hansen Birgitte , Sigsgaard Torben , Kolstad Henrik Albert , Bonde Jens Peter Ellekilde , Ramlau-Hansen Cecilia Høst
Published: November 2022

Animal studies indicate deleterious effects of nitrate exposure on fecundity, but effects in humans are unknown, both for the prenatal and postnatal periods. We aimed to investigate if exposure to nitrate in maternal drinking water during the sensitive period of fetal life is associated with measures of fecundity in the adult sons. In a sub-analysis, the potential effects of nitrate exposure in adulthood were investigated. This cohort included 985 young adult men enrolled in The Fetal Programming of Semen Quality Cohort (FEPOS). Semen characteristics, testes volume and reproductive hormones were analyzed in relation to nitrate concentration in maternal drinking water, using a negative binomial regression model. The nitrate concentration in drinking water was obtained from monitoring data from Danish waterworks that were linked with the mothers’ residential address during pregnancy. The median nitrate concentration in maternal drinking water was 2 mg/L. At these low exposure levels, which are far below the World Health Organization’s (WHO) guideline value of 50 mg/L, we did not find indications of harmful effects of nitrate on the investigated measures of male fecundity.
Farmers’ Preference for Participating in Rural Solid Waste Management: A Case Study from Shaanxi Province, China
Authors: Wu Wenyan , Li Lu , Chen Hanxin , Xu Minyue , Yuan Yalin
Published: November 2022

Rural solid waste management is an important method to improve rural living environments. Farmers’ participation in rural solid waste management plays an essential role in sustainable waste management. Based on the micro-survey data of 592 farmers in Shaanxi province, a multinomial logit model was applied to explore farmers’ preferences for participating in rural solid waste management. The empirical results show that both institutional and interpersonal trust have significant positive effects on farmers’ payment participation, and labor and payment participation. Among environmental awareness, farmers with pro-environmental behavior prefer labor participation, and labor and payment participation; the more environmental knowledge farmers have, the stronger their preferences for payment participation, labor and payment participation, and labor participation; farmers concerned about environmental problems are more inclined to adopt labor and payment participation or payment participation. For socio-demographic characteristics, gender has no significant influence, while agricultural net income and education can significantly increase farmers’ willingness to participate; farmers who have migrant work experience prefer to participate in payment; there are obvious intergenerational differences in the influence of social trust and environmental awareness on farmers’ participation preference. Therefore, providing diversified participation modes, creating a good social trust environment, and enhancing farmers’ awareness of environmental care are important in promoting rural solid waste management.
Knowledge, Understanding and Satisfaction with the Implementation of the Performance Management System at a District Hospital in the Madibeng Subdistrict, South Africa
Authors: Thobejane Gontse , van der Heever Hendry , Mokgatle Mathildah
Published: November 2022

The Performance Management and Development System (PMDS) is an essential asset in aligning the strategic objectives of both the National and Provincial departments of health with the individual targets and Key Responsibility Areas (KRAs) of the individual employees working in these departments. The system is not without challenges. The sources of contention can be with the rewards mechanism of the system, bias in the application of the system and the incorrect use of the system for punitive measures. The objective of this study was to determine the perceived knowledge, understanding and satisfaction levels of employees at Brits District Hospital regarding the PMDS. Another objective was to provide an intervention targeting the knowledge and understanding of the key concepts of the system. A quantitative cross-sectional and longitudinal action research method was applied by tracking the responses of 64 healthcare workers three times, firstly in a questionnaire and then in an intervention checklist. The respondents were selected via proportionate stratified sampling. Data were analysed via STATA statistical software package 13.0. The respondents had a poor knowledge and understanding of how to score the PMDS. The respondents where highly dissatisfied with the development of their weaknesses and the recognition of their strengths. Training needs to be prioritized on both the PMDS and the employees’ areas of weakness affecting their performance toward their assigned Key Responsibility Areas. Employees need to be recognised for areas in which they show strength.
Oxidative Stress-Related Signaling Pathways Predict Oocytes’ Fertilization In Vitro and Embryo Quality
Authors: Artini Paolo Giovanni , Scarfò Giorgia , Marzi Ilaria , Fusi Jonathan , Obino Maria Elena , Franzoni Ferdinando , Zappelli Elisa , Chelucci Elisa , Martini Claudia , Cela Vito , Daniele Simona
Published: November 2022

Oocyte development and fertilization are largely influenced by the microenvironment of the follicular fluid (FF), and the exploration of its molecular/metabolic composition may help in improving in vitro fertilization (IVF) outcomes. Here, the concentrations of molecules related to oxidative stress/inflammation were measured in FF from follicles at oocyte retrieval during IVF. Here, the FF antioxidant potential was correlated with the number of retrieved/mature oocytes and the number of fertilized ones. FF collected from the follicles of normal fertilized oocytes presented an elevated antioxidant capability, lower levels of pro-inflammatory molecules (i.e., IL-6, IL-8, IL-12, TGF-β, and HIF-1α), and a higher IL-10 concentration. FF samples from follicles at oocyte retrieval that resulted in top-quality embryos displayed a peculiar antioxidant capability and a further decrease in proinflammatory molecules when compared with FF, giving rise to poor-quality embryos. Finally, pro-inflammatory molecules were lower and accompanied by a high antioxidant capability in samples giving rise to successful embryo implantation. The antioxidant capability and IL-10 displayed a good predictive ability for fertilization and embryo quality. Overall, our data showed the great influence of oxidative stress on the oocytes’ fertilization, and shed light on the importance of controlling the inflammatory and oxidative status of FF to obtain good-quality embryos with significant implantation potential.
Comparative Preclinical Evaluation of Peptide-Based Chelators for the Labeling of DARPin G3 with 99mTc for Radionuclide Imaging of HER2 Expression in Cancer
Authors: Larkina Mariia , Plotnikov Evgenii , Bezverkhniaia Ekaterina , Shabanova Yulia , Tretyakova Maria , Yuldasheva Feruza , Zelchan Roman , Schulga Alexey , Konovalova Elena , Vorobyeva Anzhelika , Garousi Javad , Gräslund Torbjörn , Belousov Mikhail , Tolmachev Vladimir , Deyev Sergey
Published: November 2022

Non-invasive radionuclide imaging of human epidermal growth factor receptor type 2 (HER2) expression in breast, gastroesophageal, and ovarian cancers may stratify patients for treatment using HER2-targeted therapeutics. Designed ankyrin repeat proteins (DARPins) are a promising type of targeting probe for radionuclide imaging. In clinical studies, the DARPin [99mTc]Tc-(HE)3-G3 labeled using a peptide-based chelator His-Glu-His-Glu-His-Glu ((HE)3), provided clear imaging of HER2 expressing breast cancer 2–4 h after injection. The goal of this study was to evaluate if the use of cysteine-containing peptide-based chelators Glu-Glu-Glu-Cys (E3C), Gly-Gly-Gly-Cys (G3C), and Gly-Gly-Gly-Ser-Cys connected via a (Gly-Gly-Gly-Ser)3-linker (designated as G3-(G3S)3C) would further improve the contrast of imaging using 99mTc-labeled derivatives of G3. The labeling of the new variants of G3 provided a radiochemical yield of over 95%. Labeled G3 variants bound specifically to human HER2-expressing cancer cell lines with affinities in the range of 1.9–5 nM. Biodistribution of [99mTc]Tc-G3-G3C, [99mTc]Tc-G3-(G3S)3C, and [99mTc]Tc-G3-E3C in mice was compared with the biodistribution of [99mTc]Tc-(HE)3-G3. It was found that the novel variants provide specific accumulation in HER2-expressing human xenografts and enable discrimination between tumors with high and low HER2 expression. However, [99mTc]Tc-(HE)3-G3 provided better contrast between tumors and the most frequent metastatic sites of HER2-expressing cancers and is therefore more suitable for clinical applications.
Involvement of Atopic Dermatitis in the Development of Systemic Inflammatory Diseases
Authors: Itamura Misa , Sawada Yu
Published: November 2022

The skin is recognized as a peripheral lymphoid organ that plays an essential defensive action against external environmental stimuli. However, continuous stimulation of these factors causes chronic inflammation at the local site and occasionally causes tissue damage. Chronic inflammation is recognized as a trigger for systemic organ inflammation. Atopic dermatitis (AD) is a chronic inflammatory skin disease that is influenced by various external environmental factors, such as dry conditions, chemical exposure, and microorganisms. The pathogenesis of AD involves various Th2 and proinflammatory cytokines. Recently updated studies have shown that atopic skin-derived cytokines influence systemic organ function and oncogenesis. In this review, we focus on AD’s influence on the development of systemic inflammatory diseases and malignancies.
Functional Conservation and Divergence of MOS1 That Controls Flowering Time and Seed Size in Rice and Arabidopsis
Authors: Lu Shan , Zhang Ning , Xu Yazhen , Chen Hao , Huang Jie , Zou Baohong
Published: November 2022

The heading date and grain size are two essential traits affecting rice yield. Here, we found that OsMOS1 promotes rice heading and affects its grain size. Knocking out OsMOS1 delayed heading, while the overexpression of OsMOS1 promoted heading in rice under long-day conditions. The transcriptions of the heading activators Ehd1, Hd3a, and RFT1 were decreased and the heading repressor Hd1 was increased in the osmos1 mutant. Conversely, the overexpression of OsMOS1 promoted the expressions of Ehd1, Hd3a, and RFT1, but inhibited the expression of Hd1. This suggests that OsMOS1 may control heading in rice by modulating the transcriptions of Ehd1, Hd3a, RFT1, and Hd1. In addition, knocking out OsMOS1 led to larger grains with longer grain lengths and higher grain weights. The seed cell size measurement showed that the cell lengths and cell widths of the outer glume epidermal cells of the osmos1 mutant were greater than those of the wild type. Furthermore, we also found that the overexpression of OsMOS1 in the Arabidopsis mos1 mutant background could suppress its phenotypes of late flowering and increased seed size. Thus, our study shows a conserved function of MOS1 in rice and Arabidopsis, and these findings shed light on the heading and seed size regulation in rice and suggest that OsMOS1 is a promising target for rice yield improvement.
Organokines, Sarcopenia, and Metabolic Repercussions: The Vicious Cycle and the Interplay with Exercise
Authors: Minniti Giulia , Pescinini-Salzedas Letícia Maria , Minniti Guilherme Almeida dos Santos , Laurindo Lucas Fornari , Barbalho Sandra Maria , Vargas Sinatora Renata , Sloan Lance Alan , Haber Rafael Santos de Argollo , Araújo Adriano Cressoni , Quesada Karina , Haber Jesselina F. dos Santos , Bechara Marcelo Dib , Sloan Katia Portero
Published: November 2022

Sarcopenia is a disease that becomes more prevalent as the population ages, since it is directly linked to the process of senility, which courses with muscle atrophy and loss of muscle strength. Over time, sarcopenia is linked to obesity, being known as sarcopenic obesity, and leads to other metabolic changes. At the molecular level, organokines act on different tissues and can improve or harm sarcopenia. It all depends on their production process, which is associated with factors such as physical exercise, the aging process, and metabolic diseases. Because of the seriousness of these repercussions, the aim of this literature review is to conduct a review on the relationship between organokines, sarcopenia, diabetes, and other metabolic repercussions, as well the role of physical exercise. To build this review, PubMed-Medline, Embase, and COCHRANE databases were searched, and only studies written in English were included. It was observed that myokines, adipokines, hepatokines, and osteokines had direct impacts on the pathophysiology of sarcopenia and its metabolic repercussions. Therefore, knowing how organokines act is very important to know their impacts on age, disease prevention, and how they can be related to the prevention of muscle loss.
Proteomics of Aqueous Humor as a Source of Disease Biomarkers in Retinoblastoma
Authors: Galardi Angela , Stathopoulos Christina , Colletti Marta , Lavarello Chiara , Russo Ida , Cozza Raffaele , Romanzo Antonino , Carcaboso Angel M. , Locatelli Franco , Petretto Andrea , Munier Francis L. , Di Giannatale Angela
Published: November 2022

Aqueous humor (AH) can be easily and safely used to evaluate disease-specific biomarkers in ocular disease. The aim of this study was to identify specific proteins biomarkers in the AH of retinoblastoma (RB) patients at various stages of the disease. We analyzed the proteome of 53 AH samples using high-resolution mass spectrometry. We grouped the samples according to active vitreous seeding (Group 1), active aqueous seeding (Group 2), naive RB (group 3), inactive RB (group 4), and congenital cataracts as the control (Group 5). We found a total of 889 proteins in all samples. Comparative parametric analyses among the different groups revealed three additional proteins expressed in the RB groups that were not expressed in the control group. These were histone H2B type 2-E (HISTH2B2E), InaD-like protein (PATJ), and ubiquitin conjugating enzyme E2 V1 (UBE2V1). Upon processing the data of our study with the OpenTarget Tool software, we found that glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and CD44 were more highly expressed in the RB groups. Our results provide a proteome database regarding AH related to RB disease that may be used as a source of biomarkers. Further prospective studies should validate our finding in a large cohort of RB patients.
Physiological Impact of a Synthetic Elastic Protein in Arterial Diseases Related to Alterations of Elastic Fibers: Effect on the Aorta of Elastin-Haploinsufficient Male and Female Mice
Authors: Boëté Quentin , Lo Ming , Liu Kiao-Ling , Vial Guillaume , Lemarié Emeline , Rougelot Maxime , Steuckardt Iris , Harki Olfa , Couturier Axel , Gaucher Jonathan , Bouyon Sophie , Demory Alexandra , Boutin-Paradis Antoine , El Kholti Naima , Berthier Aurore , Pépin Jean-Louis , Briançon-Marjollet Anne , Lambert Elise , Debret Romain , Faury Gilles
Published: November 2022

Elastic fibers, made of elastin (90%) and fibrillin-rich microfibrils (10%), are the key extracellular components, which endow the arteries with elasticity. The alteration of elastic fibers leads to cardiovascular dysfunctions, as observed in elastin haploinsufficiency in mice (Eln+/-) or humans (supravalvular aortic stenosis or Williams–Beuren syndrome). In Eln+/+ and Eln+/- mice, we evaluated (arteriography, histology, qPCR, Western blots and cell cultures) the beneficial impact of treatment with a synthetic elastic protein (SEP), mimicking several domains of tropoelastin, the precursor of elastin, including hydrophobic elasticity-related domains and binding sites for elastin receptors. In the aorta or cultured aortic smooth muscle cells from these animals, SEP treatment induced a synthesis of elastin and fibrillin-1, a thickening of the aortic elastic lamellae, a decrease in wall stiffness and/or a strong trend toward a reduction in the elastic lamella disruptions in Eln+/- mice. SEP also modified collagen conformation and transcript expressions, enhanced the aorta constrictive response to phenylephrine in several animal groups, and, in female Eln+/- mice, it restored the normal vasodilatory response to acetylcholine. SEP should now be considered as a biomimetic molecule with an interesting potential for future treatments of elastin-deficient patients with altered arterial structure/function.