bluuebunny/crossref_metadata_2025 huggingface dataset:
Finding Meanings in Low Dimensional Structures: Stochastic Neighbor Embedding Applied to the Analysis of Indri indri Vocal Repertoire
Authors: Valente Daria , De Gregorio Chiara , Torti Valeria , Miaretsoa Longondraza , Friard Olivier , Randrianarison Rose Marie , Giacoma Cristina , Gamba Marco
Published: May 2019

Although there is a growing number of researches focusing on acoustic communication, the lack of shared analytic approaches leads to inconsistency among studies. Here, we introduced a computational method used to examine 3360 calls recorded from wild indris (Indri indri) from 2005–2018. We split each sound into ten portions of equal length and, from each portion we extracted spectral coefficients, considering frequency values up to 15,000 Hz. We submitted the set of acoustic features first to a t-distributed stochastic neighbor embedding algorithm, then to a hard-clustering procedure using a k-means algorithm. The t-distributed stochastic neighbor embedding (t-SNE) mapping indicated the presence of eight different groups, consistent with the acoustic structure of the a priori identification of calls, while the cluster analysis revealed that an overlay between distinct call types might exist. Our results indicated that the t-distributed stochastic neighbor embedding (t-SNE), successfully been employed in several studies, showed a good performance also in the analysis of indris’ repertoire and may open new perspectives towards the achievement of shared methodical techniques for the comparison of animal vocal repertoires.
Hair Cortisol in Sheltered Cows and Its Association with Other Welfare Indicators
Authors: Sharma Arvind , Umapathy Govindhaswamy , Kumar Vinod , Phillips Clive J. C.
Published: May 2019

India, the country with the largest population of dairy cows in the world, has a policy of retiring abandoned and non-lactating cows in shelters, but the level of provision for their welfare in these shelters is unclear. Cows in 54 shelters across India were assessed for historic evidence of physiological stress, through determination of hair cortisol in 540 samples from 10 cows in each shelter by enzyme immunoassay. Animal-based and shelter resource-based welfare measures were recorded and correlations with the hair cortisol investigated by multivariable analysis. High hair cortisol concentrations were associated with dung in the lying area of the cowshed, a low dry bulb temperature there and little cow access to yards, as shelter-based variables. At a cow level, high hair cortisol concentrations were associated with dirty flanks, hock joint ulceration, carpal joint injuries, body lesions, dehydration, an empty rumen, old age, and low levels of body hair loss. Hair cortisol level promises to be an effective biomarker of stress in cows when conducting studies under field conditions.
Explanation of Osteoblastic Differentiation of Stem Cells by Photo Biomodulation Using the Resonant Recognition Model
Authors: Cosic Irena , Paspaliaris Vasilis , Cosic Drasko
Published: May 2019

Differentiation of stem cells into different tissues is a promising approach to treat a large number of diseases, as well as for tissue transplantation and repair. It has been shown that parathyroid hormone, similarly to stromal self-derived factor, and the radiation of specific electromagnetic frequencies of blue and green light, can encourage stem cell differentiation into osteoblasts. Here, we analysed parathyroid hormone, its receptor and stromal self-derived factor using the Resonant Recognition Model, which proposes that protein function is based on specific frequencies of electromagnetic radiation within ultra-violet, visible, infra-red and far infra-red light. The purpose of this research is to predict the characteristic frequencies related to parathyroid hormone activities, particularly differentiation of stem cells into osteoblasts. We have found that the most effective wavelength for stem cell differentiation would be 502 nm, which is between 420 nm and 540 nm, already experimentally proven to be effective in stimulating osteoblast differentiation. Thus, we propose that wavelength radiation of 502 nm will be even more efficient for differentiation of stem cells into osteoblasts.
Experimental Study on Flexural Behavior of TRM-Strengthened RC Beam: Various Types of Textile-Reinforced Mortar with Non-Impregnated Textile
Authors: Park Jongho , Hong Sungnam , Park Sun-Kyu
Published: May 2019

In this study, to compare strengthening efficiency and flexural behaviors of textile- reinforced mortar (TRM) according to various types of strengthening methods without the textile being impregnated, ten specimens were tested. The results showed that TRM was beneficial for uniform distribution of cracks and increased the strengthening efficiency and load-bearing capacity, as textile reinforcement ratio and textile lamination increased and the mesh size of the textile decreased and mechanical end anchorage applied. However, the strengthening effect was shown obviously until the yield load considering structural safety and serviceability.
Ant Lion Optimized Fractional Order Fuzzy Pre-Compensated Intelligent Pid Controller for Frequency Stabilization of Interconnected Multi-Area Power Systems
Authors: Gomaa Haroun A. H. , Li Yin-Ya
Published: May 2019

Load frequency control (LFC) is considered to be the most important strategy in interconnected multi-area power systems for satisfactory operation and distribution. In order to transfer reliable power with acceptable quality, an LFC mechanism requires highly efficacy and intelligent techniques. In this paper, a novel hybrid fractional order fuzzy pre-compensated intelligent proportional-integral-derivative (PID) (FOFP-iPID) controller is proposed for the LFC of a realistic interconnected two-area power system. The proposed FOFP-iPID controller is incorporated into the power system as a secondary controller. In doing so, the parameters of the suggested FOFP-iPID controller are optimized using a more recent evolutionary computational technique called the Ant lion optimizer (ALO) algorithm utilizing an Integral of Time multiplied Absolute Error (ITAE) index. Simulation results demonstrated that the proposed FOFP-iPID controller achieves better dynamics performance under a wide variation of load perturbations. The supremacy of the proposed FOFP-iPID controller is demonstrated by comparing the results with some existing controllers, such as fractional order PID (FOPID) and fractional order intelligent PID (FOiPID) controllers for the identical system. Finally, the sensitivity analysis of the plant is examined and the simulation results showed that the suggested FOFP-iPID controller is robust and performs satisfactorily despite the presence of uncertainties.
Clinical Significance of Lymph-Node Ratio in Determining Supraclavicular Lymph-Node Radiation Therapy in pN1 Breast Cancer Patients Who Received Breast-Conserving Treatment (KROG 14-18): A Multicenter Study
Authors: Kim Jaeho , Park Won , Kim Jin , Choi Doo , Kim Yeon-Joo , Lee Eun , Shin Kyung , Kim Jin , Kim Kyubo , Kim Yong , Ahn Sung-Ja , Lee Jong , Chun Mison , Lee Hyung-Sik , Kim Jung , Cha Jihye
Published: May 2019

This study evaluated the clinical significance of the lymph-node ratio (LNR) and its usefulness as an indicator of supraclavicular lymph-node radiation therapy (SCNRT) in pN1 breast cancer patients with disease-free survival (DFS) outcomes. We retrospectively analyzed the clinical data of patients with pN1 breast cancer who underwent partial mastectomy and taxane-based sequential adjuvant chemotherapy with postoperative radiation therapy in 12 hospitals (n = 1121). We compared their DFS according to LNR, with a cut-off value of 0.10. The median follow-up period was 66 months (range, 3–112). Treatment failed in 73 patients (6.5%) and there was no significant difference in DFS between the SCNRT group and non-SCNRT group. High LNR (>0.10) showed significantly worse DFS in both univariate and multivariate analyses (0.010 and 0.033, respectively). In a subgroup analysis, the effect of SCNRT on DFS differed significantly among patients with LNR > 0.10 (p = 0.013). High LNR can be used as an independent prognostic factor for pN1 breast cancer patients treated with partial mastectomy and postoperative radiotherapy. It may also be useful in deciding whether to perform SCNRT to improve DFS.
CD4+/CD45RO+: A Potential Biomarker of the Clinical Response to Glatiramer Acetate
Authors: Vališ Martin , Sobíšek Lukáš , Vyšata Oldřich , Klímová Blanka , Andrýs Ctirad , Vokurková Doris , Masopust Jiří , Pavelek Zbyšek
Published: May 2019

Background: Glatiramer acetate (GA) is an effective treatment for the earliest stages of multiple sclerosis (MS)—clinically isolated syndrome (CIS) or clinically definite MS (CDMS). Objective: This study aims to determine the differences in the lymphocyte population (at baseline and the course of five years) between confirmed sustained progression (CSP) and non-CSP groups and to identify potential biomarkers among these parameters that can predict a positive response to the treatment. Methods: Twelve male and 60 female patients were included in the study. Peripheral blood samples were collected before and five years after treatment with GA. The authors compared lymphocyte parameters between the CSP and non-CSP groups by statistical analyses. Univariate and penalized logistic regression models were fitted to identify the best lymphocyte parameters at baseline and their combination for potential biomarkers. Subsequently, the ROC analysis was used to identify cut-offs for selected parameters. Results: The parameter CD4+/CD45RO+ was identified as the best single potential biomarker, demonstrating the ability to identify patients with CSP. Moreover, a combination of four lymphocyte parameters at baseline, relative lymphocyte counts, CD3+/CD69+, CD4+/CD45RO+, and CD4+/CD45RA+ab, was identified as a potential composite biomarker. This combination explains 23% of the variability in CSP, which is better than the best univariate parameter when compared to CD4+/CD45RO+ at baseline. Conclusions: The results suggest that other biomarkers can help monitor the conditions of patients and predict a favourable outcome.
Preconditioning in an Inflammatory Milieu Augments the Immunotherapeutic Function of Mesenchymal Stromal Cells
Authors: Rodriguez Luis A. , Mohammadipoor Arezoo , Alvarado Lucero , Kamucheka Robin M. , Asher Amber M. , Cancio Leopoldo C. , Antebi Ben
Published: May 2019

Multipotent mesenchymal stromal cells (MSCs) have emerged as potent therapeutic agents for multiple indications. However, recent evidence indicates that MSC function is compromised in the physiological post-injury milieu. In this study, bone marrow (BM)- and adipose-derived (AD)-MSCs were preconditioned in hypoxia with or without inflammatory mediators to potentiate their immunotherapeutic function in preparation for in vivo delivery. Human MSCs were cultured for 48 h in either normoxia (21% O2) or hypoxia (2% O2) with or without the addition of Cytomix, thus creating 4 groups: (1) normoxia (21%); (2) Cytomix-normoxia (+21%); (3) hypoxia (2%); and (4) Cytomix-hypoxia (+2%). The 4 MSC groups were subjected to comprehensive evaluation of their characteristics and function. Preconditioning did not alter common MSC surface markers; nonetheless, Cytomix treatment triggered an increase in tissue factor (TF) expression. Moreover, the BM-MSCs and AD-MSCs from the +2% group were not able to differentiate to chondrocytes and osteoblasts, respectively. Following Cytomix preconditioning, the metabolism of MSCs was significantly increased while viability was decreased in AD-MSCs, but not in BM-MSCs. MSCs from both tissues showed a significant upregulation of key anti-inflammatory genes, increased secretion of IL-1 receptor antagonist (RA), and enhanced suppression of T-cell proliferation following the Cytomix treatment. Similarly, following a lipopolysaccharide challenge, the Cytomix-treated MSCs suppressed TNF-α secretion, while promoting the production of IL-10 and IL-1RA. These preconditioning approaches facilitate the production of MSCs with robust anti-inflammatory properties. AD-MSCs preconditioned with Cytomix under normoxia appear to be the most promising therapeutic candidates; however, safety concerns, such as thrombogenic disposition of cells due to TF expression, should be carefully considered prior to clinical translation.
Actin Cytoskeleton Straddling the Immunological Synapse between Cytotoxic Lymphocytes and Cancer Cells
Authors: Wurzer Hannah , Hoffmann Céline , Al Absi Antoun , Thomas Clément
Published: May 2019

The immune system is a fundamental part of the tumor microenvironment. In particular, cytotoxic lymphocytes, such as cytolytic T cells and natural killer cells, control tumor growth and disease progression by interacting and eliminating tumor cells. The actin cytoskeleton of cytotoxic lymphocytes engaged in an immunological synapse has received considerable research attention. It has been recognized as a central mediator of the formation and maturation of the immunological synapse, and its signaling and cytolytic activities. In comparison, fewer studies have explored the organization and function of actin filaments on the target cancer cell side of the immunological synapse. However, there is growing evidence that the actin cytoskeleton of cancer cells also undergoes extensive remodeling upon cytotoxic lymphocyte attack, and that such remodeling can alter physical and functional interactions at the immunological synapse. In this article, we review the current knowledge of actin organization and functions at both sides of the immunological synapse between cytotoxic lymphocytes and cancer cells, with particular focus on synapse formation, signaling and cytolytic activity, and immune evasion.
Advances in Regenerative Stem Cell Therapy in Androgenic Alopecia and Hair Loss: Wnt Pathway, Growth-Factor, and Mesenchymal Stem Cell Signaling Impact Analysis on Cell Growth and Hair Follicle Development
Authors: Gentile Pietro , Garcovich Simone
Published: May 2019

The use of stem cells has been reported to improve hair regrowth in several therapeutic strategies, including reversing the pathological mechanisms, that contribute to hair loss, regeneration of hair follicles, or creating hair using the tissue-engineering approach. Although various promising stem cell approaches are progressing via pre-clinical models to clinical trials, intraoperative stem cell treatments with a one-step procedure offer a quicker result by incorporating an autologous cell source without manipulation, which may be injected by surgeons through a well-established clinical practice. Many authors have concentrated on adipose-derived stromal vascular cells due to their ability to separate into numerous cell genealogies, platelet-rich plasma for its ability to enhance cell multiplication and neo-angiogenesis, as well as human follicle mesenchymal stem cells. In this paper, the significant improvements in intraoperative stem cell approaches, from in vivo models to clinical investigations, are reviewed. The potential regenerative instruments and functions of various cell populaces in the hair regrowth process are discussed. The addition of Wnt signaling in dermal papilla cells is considered a key factor in stimulating hair growth. Mesenchymal stem cell-derived signaling and growth factors obtained by platelets influence hair growth through cellular proliferation to prolong the anagen phase (FGF-7), induce cell growth (ERK activation), stimulate hair follicle development (β-catenin), and suppress apoptotic cues (Bcl-2 release and Akt activation).
Advances in Damage Monitoring Techniques for the Detection of Failure in SiCf/SiC Ceramic Matrix Composites
Authors: Bache Martin R. , Newton Christopher D. , Jones John Paul , Pattison Stephen , Gale Louise , Nicholson Pascual Ian , Weston Eleri
Published: May 2019

From a disruptive perspective, silicon carbide (SiC)-based ceramic matrix composites (CMCs) provide a considerable temperature and weight advantage over existing material systems and are increasingly finding application in aerospace, power generation and high-end automotive industries. The complex structural architecture and inherent processing artefacts within CMCs combine to induce inhomogeneous deformation and damage prior to ultimate failure. Sophisticated mechanical characterisation is vital in support of a fundamental understanding of deformation in CMCs. On the component scale, “damage tolerant” design and lifing philosophies depend upon laboratory assessments of macro-scale specimens, incorporating typical fibre architectures and matrix under representative stress-strain states. This is important if CMCs are to be utilised to their full potential within industrial applications. Bulk measurements of strain via extensometry or even localised strain gauging would fail to characterise the ensuing inhomogeneity when performing conventional mechanical testing on laboratory scaled coupons. The current research has, therefore, applied digital image correlation (DIC), electrical resistance monitoring and acoustic emission techniques to the room and high-temperature assessment of ceramic matrix composites under axial tensile and fatigue loading, with particular attention afforded to a silicon carbide fibre-reinforced silicon carbide composite (SiCf/SiC) variant. Data from these separate monitoring techniques plus ancillary use of X-ray computed tomography, in-situ scanning electron microscopy and optical inspection were correlated to monitor the onset and progression of damage during mechanical loading. The benefits of employing a concurrent, multi-technique approach to monitoring damage in CMCs are demonstrated.
A Deformed Exponential Statistical Manifold
Authors: Josué Vieira Francisca Leidmar , Félix de Andrade Luiza Helena , Facundo Vigelis Rui , Casimiro Cavalcante Charles
Published: May 2019

Consider μ a probability measure and P μ the set of μ -equivalent strictly positive probability densities. To endow P μ with a structure of a C ∞ -Banach manifold we use the φ -connection by an open arc, where φ is a deformed exponential function which assumes zero until a certain point and from then on is strictly increasing. This deformed exponential function has as particular cases the q-deformed exponential and κ -exponential functions. Moreover, we find the tangent space of P μ at a point p, and as a consequence the tangent bundle of P μ . We define a divergence using the q-exponential function and we prove that this divergence is related to the q-divergence already known from the literature. We also show that q-exponential and κ -exponential functions can be used to generalize of Rényi divergence.
The Voltage Control Strategy of a DC-Link Bus Integrated Photovoltaic Charging Module in a Unified Power Quality Conditioner
Authors: Ni Fuyin , Li Zhengming , Wang Qi
Published: May 2019

In order to improve the functionality and efficiency of a unified power quality conditioner (UPQC), a DC-link bus integrated photovoltaic charging module is proposed in a UPQC. It can generate power for essential loads apart from providing energy to a DC-link bus. A conventional proportional integral (PI) controller fails to run smoothly in dynamic conditions of the micro-grid, since it has poor capabilities in determining suitable values of proportional gain and integral gain. So, the optimization algorithm for a PI controller based on chaos particle swarm optimization based on a multi-agent system (CPSO-MAS) algorithm was developed in this paper to overcome properties such as intermittent instability in the micro-grid. Through verification by simulation and experiment of UPQC harmonic compensation, it showed that the proposed DC link bus voltage control strategy can be effectively applied to UPQC towards various conditions related to voltage and current distortion. In addition, it proved that the proposed strategy has faster convergence than other algorithms, which enhances the stability of DC-link bus voltage. Hence, the contribution presented in this paper is to provide a novel approach for the power quality improvement of UPQC.
Research on Cooperative Planning of Distributed Generation Access to AC/DC Distribution (Micro) Grids Based on Analytical Target Cascading
Authors: Pan Hao , Ding Ming , Bi Rui , Sun Lei
Published: May 2019

With the wide application of distributed generation (DG) and the rapid development of alternating current/direct current (AC/DC) hybrid microgrids, the optimal planning of distributed generation connecting to AC/DC distribution networks/microgrids has become an urgent problem to resolve. This paper presents a collaborative planning method for distributed generation access to AC/DC distribution (micro) grids. Based on the grid structure of the AC/DC distribution network, the typical interconnection structure of the AC/DC hybrid microgrid and AC/DC distribution network is designed. The optimal allocation models of distributed power supply for the AC/DC distribution network and microgrid are established based on analytical target cascading. The power interaction between the distribution network and microgrid is used to establish a coupling relationship, and the augmented Lagrangian penalty function is used to solve the collaborative programming problem. The results of distributed power supply allocation are obtained, solving the problem so that distribution generation with different capacity levels is connected to the power grid system in a single form.
Integration of Electric Vehicles into the Electric Power System Based on Results of Road Traffic Census
Authors: Iwafune Yumiko , Ogimoto Kazuhiko , Azuma Hitoshi
Published: May 2019

We propose a model for the integration of electric vehicles (EVs) into the grid power system in Japan. The potential of the switchover from conventional vehicles to EVs and the incurred charging loads for the EV fleet were evaluated based on the results of a Japanese road traffic census. Furthermore, an EV battery operation model was incorporated into the production cost analysis model, which is capable of determining the optimal electricity supply and demand, considering the existing interconnector power flows. The potential economic and environmental contributions of EV charge and discharge controls, with the ultimate goal of realizing the introduction of a massive renewable energy source in the future, were also evaluated. We found that EVs can greatly contribute to expanding the use of renewable energy and reducing system cost by charging and discharging not only at the owner’s home but also at his/her workplace.
ZVS Auxiliary Circuit for a 10 kW Unregulated LLC Full-Bridge Operating at Resonant Frequency for Aircraft Application
Authors: Bouvier Yann E. , Serrano Diego , Borović Uroš , Moreno Gonzalo , Vasić Miroslav , Oliver Jesús A. , Alou Pedro , Cobos José A. , Carmena Jorge
Published: May 2019

In modern aircraft designs, following the More Electrical Aircraft (MEA) philosophy, there is a growing need for new high-power converters. In this context, innovative solutions to provide high efficiency and power density are required. This paper proposes an unregulated LLC full-bridge operating at resonant frequency to obtain a constant gain at all loads. The first harmonic approximation (FHA) model is not accurate enough to estimate the voltage gain in converters with high parasitic resistance. A modified FHA model is proposed for voltage gain analysis, and time-based models are used to calculate the instantaneous current required for the ZVS transition analysis. A method using charge instead of current is proposed and used for this ZVS analysis. Using this method, an auxiliary circuit is proposed to achieve complete ZVS within the whole load range, avoiding a gapped transformer design and increasing the efficiency and power density. A 28 Vdc output voltage prototype, with 10 kW peak output power, has been developed to validate the theoretical analysis and the proposed auxiliary circuit. The maximum efficiency (96.3%) is achieved at the nominal power of 5 kW.
Design and Evaluation of a Surfactant–Mixed Metal Hydroxide-Based Drilling Fluid for Maintaining Wellbore Stability in Coal Measure Strata
Authors: Chen Shuya , Shi Yanping , Yang Xianyu , Xie Kunzhi , Cai Jihua
Published: May 2019

Co-exploitation of coal measure gases (coalbed gas, shale gas, and tight sandstone gas) puts a higher requirement on drilling fluids. Conventional drilling fluids have disadvantages, such as causing problems of borehole collapse, formation damage, and water blockage. This paper proposes a set of high inhibitive and low-damage drilling fluids that function by electrical inhibition and neutral wetting. Zeta potential results showed that the negative electrical property of Longtan coal in Bijie, Guizhou, can be reversed by organic mixed metal hydroxide (MMH) and the cationic surfactant alkyl trimethylammonium bromide (CS-5) from −3.63 mV to 19.75 mV and 47.25 mV, respectively. Based on the contact angle and Fourier Transform Infrared Spectroscopy (FT-IR) results, it can be concluded that chemical adsorption dominates between the Longmaxi shale and surfactants, and physical adsorption between the Longtan coal and surfactants. A compound surfactant formula (0.001 wt% CS-4 + 0.001 wt% CS-1 + 0.001 wt% CS-3), which could balance the wettability of the Longmaxi shale and the Longtan coal, making them both appear weakly hydrophilic simultaneously, was optimized. After being treated by the compound surfactants, the contact angles of the Longmaxi shale and the Longtan coal were 89° and 86°, respectively. Pressure transmission tests showed that the optimized combination of compound surfactants and inorganic MMH (MMH-1) could effectively reduce permeability of the Longmaxi shale and the Longtan coal, thus retarding pore pressure transmission in coal measure strata. Then, the proposed water-based drilling fluid (WBDF) system (4 wt% sodium bentonite + 1.5 wt% sodium carboxymethyl cellulose + 2 wt% lignite resin + 5 wt% potassium chloride + 3 wt%MMH-1 + 0.001 wt% CS-4 + 0.001 wt% CS-1 + 0.001 wt% CS-3) was evaluated based on parameters including rheology, American Petroleum Institute (API) filtration, electrical property, wettability, inhibition capability, reservoir protection characteristics, and anti-pollution performance. It had an API filtration of 7 mL, reservoir damage rate of 10%, moderate and acceptable viscosity, strong inhibition capability to coal measure strata rocks, good tolerance to inorganic pollutants and drilling cuttings, and environmentally friendly properties. It could meet wellbore stability and reservoir protection requirements in the co-exploitation of coal measure gases.
A Class of Energy Efficient Self-Contained Electro-Hydraulic Drives with Self-Locking Capability
Authors: Schmidt Lasse , Ketelsen Søren , Brask Morten Helms , Mortensen Kasper Aastrup
Published: May 2019

Pump controlled and self-contained electro-hydraulic cylinder drives may improve energy efficiency and reduce installation space compared to conventional valve solutions, while being in line with the trend of electrification. The topic has gained increasing interest in industry as well as in academia in recent years. However, this technology has failed to break through in industry on a broad scale, with the reason assumed to be lack of meeting industry requirements. These requirements include high drive stiffness enabling a large application range, and the ability to maintain cooling and filtration in required ranges, enabling proper reliability and durability. Furthermore, at this point the cost of realization of such drives is comparable only to high end valve drive solutions, while not providing dynamics on a similar level. An initiative to improve this technology in terms of a class of drives evolving around a hydraulic cylinder locking mechanism is proposed. The resulting class of drives generally rely on separate cylinder forward and return flow paths, allowing for fluid cooling and filtration as well as control of the drive stiffness. The proposed class of drives is analyzed regarding energy loss and recovery potential, a basic model based control design is realized, and the industrial feasibility of the drive class is considered. It is found that the proposed class of drives may be realized with standard components maintained in their design ranges at competitive costs compared to conventional valve solutions. Furthermore, it is found that pressure levels may be controlled in a proper way, allowing to produce either highly efficient operation or a high drive stiffness.
Some Investigations on a Possible Relationship between Ground Deformation and Seismic Activity at Campi Flegrei and Ischia Volcanic Areas (Southern Italy)
Authors: Ricco Ciro , Petrosino Simona , Aquino Ida , Del Gaudio Carlo , Falanga Mariarosaria
Published: May 2019

In the present paper, we analyse ground tilt and seismicity at Campi Flegrei caldera and Ischia Island, two volcanic areas located in the south of Italy. These areas have been well studied for many years from a petrological, volcanological and geophysical view point. Moreover, due to the high seismic and volcanic risk for the populations living there, they are continuously monitored by networks of geophysical and geochemical sensors. We summarize the most important results that we obtained so far, concerning the observations of relationships between seismic activity and ground tilt anomalies, focusing on the time interval 2015–2018. First, we present a detailed description of the tiltmeter and seismic networks in both the investigated areas, as well as their development and improvement over time that has enabled high quality data collection. From the joint analysis of the seismic and borehole tiltmeter signals, we often notice concurrence between tilt pattern variations and the occurrence of seismicity. Moreover, the major tilt anomalies appear to be linked with the rate and energy of volcano-tectonic earthquakes, as well as with exogenous phenomena like solid Earth tides and hydrological cycles. The analysis that we present has potential applicability to other volcanic systems. Our findings show how the joint use tilt and seismic data can contribute to better understanding of the dynamics of volcanoes.
Magnetic Properties and Redox State of Impact Glasses: A Review and New Case Studies from Siberia
Authors: Rochette Pierre , Bezaeva Natalia S. , Kosterov Andrei , Gattacceca Jérôme , Masaitis Victor L. , Badyukov Dmitry D. , Giuli Gabriele , Lepore Giovani Orazio , Beck Pierre
Published: May 2019

High velocity impacts produce melts that solidify as ejected or in-situ glasses. We provide a review of their peculiar magnetic properties, as well as a new detailed study of four glasses from Siberia: El’gygytgyn, Popigai, urengoites, and South-Ural glass (on a total of 24 different craters or strewn-fields). Two types of behavior appear: 1) purely paramagnetic with ferromagnetic impurities at most of the order of 10 ppm; this corresponds to the five tektite strewn-fields (including the new one from Belize), urengoites, and Darwin glass. Oxidation state, based in particular on X-ray spectroscopy, is mostly restricted to Fe2+; 2) variable and up to strong ferromagnetic component, up to the 1 wt % range, mostly due to substituted magnetite often in superparamagnetic state. Accordingly, bulk oxidation state is intermediate between Fe2+ and Fe3+, although metallic iron, hematite, and pyrrhotite are sometimes encountered. Various applications of these magnetic properties are reviewed in the field of paleomagnetism, magnetic anomalies, recognition of glass origin, and formation processes.
Health Risk Assessment of Different Heavy Metals Dissolved in Drinking Water
Authors: Hussain Sajjad , Habib-Ur-Rehman Muhammad , Khanam Tasawar , Sheer Abbas , Kebin Zhang , Jianjun Yang
Published: May 2019

Water pollution is a major threat to public health worldwide. The health risks of ingesting trace elements in drinking water were assessed in the provinces of Punjab and Khyber Pakhtunkhwa, Pakistan. Eight trace elements were measured in drinking water, using Inductively Coupled Plasma Mass Spectrometry (ICP-MS), and compared with permissible limits established by the World Health Organization (WHO) and the Pakistan Environmental Protection Agency (Pak EPA). In addition, health risk indicators such as the chronic daily intake (CDI) and the health risk index (HRI) were calculated. Our results showed that the concentrations of chromium (Cr), nickel (Ni), and manganese (Mn) were 2593, 1306, and 695 ng/g, respectively, in Lahore and Jhang, while the concentrations of arsenic (As) in Lahore, Vehari, Multan, and Jhang were 51, 50.4, 24, and 22 ng/g, respectively, which were higher than the permissible limits suggested by the WHO. The values of CDI were found to be in the order of Cr > Ni > Mn > Cu > As > Pb > Co > Cd. Similarly, the health risk index (HRI) values exceeded the safe limits (>1) in many cities (eg, Cr and Ni in Lahore and As in Vehari, Jhang, Lahore, and Multan). The aforementioned analysis shows that consumption of trace element-contaminated water poses an emerging health danger to the populations of these localities. Furthermore, inter-metal correlation and principal component analysis (PCA) showed that both anthropogenic and geologic activities were primary sources of drinking water contamination in the investigated areas.
Urbanization Impacts the Physicochemical Characteristics and Abundance of Fecal Markers and Bacterial Pathogens in Surface Water
Authors: Yuan Tianma , Vadde Kiran Kumar , Tonkin Jonathan D. , Wang Jianjun , Lu Jing , Zhang Zimeng , Zhang Yixin , McCarthy Alan J. , Sekar Raju
Published: May 2019

Urbanization is increasing worldwide and is happening at a rapid rate in China in line with economic development. Urbanization can lead to major changes in freshwater environments through multiple chemical and microbial contaminants. We assessed the impact of urbanization on physicochemical characteristics and microbial loading in canals in Suzhou, a city that has experienced rapid urbanization in recent decades. Nine sampling locations covering three urban intensity classes (high, medium and low) in Suzhou were selected for field studies and three locations in Huangshan (natural reserve) were included as pristine control locations. Water samples were collected for physicochemical, microbiological and molecular analyses. Compared to medium and low urbanization sites, there were statistically significant higher levels of nutrients and total and thermotolerant coliforms (or fecal coliforms) in highly urbanized locations. The effect of urbanization was also apparent in the abundances of human-associated fecal markers and bacterial pathogens in water samples from highly urbanized locations. These results correlated well with land use types and anthropogenic activities at the sampling sites. The overall results indicate that urbanization negatively impacts water quality, providing high levels of nutrients and a microbial load that includes fecal markers and pathogens.
Physical Activity During Pregnancy is Associated with Improved Breastfeeding Outcomes: A Prospective Cohort Study
Authors: Nguyen Phung Thi Hoang , Binns Colin W. , Nguyen Cong Luat , Van Ha Anh Vo , Chu Khac Tan , Duong Dat Van , Do Dung Van , Lee Andy H.
Published: May 2019

Physical activity is important for health, but little is known about associations between physical activity during pregnancy and breastfeeding. The aim of this study was to investigate any association between antenatal physical activity and breastfeeding duration. A prospective cohort of 2030 Vietnamese women, recruited between 24 and 28 week-gestation was followed up to twelve months postpartum. Physical activity was determined using the pregnancy physical activity questionnaire at baseline interview. Data was available for 1715 participants at 12 months, a 15.5% attrition rate. At 12 months 71.8% of mothers were still breastfeeding. A total of 20.9% women met physical activity targets and those mothers undertaking higher levels of physical activity had a lower risk of breastfeeding cessation by twelve months [hazard ratios HR = 0.59 (95% CI 0.47–0.74), p < 0.001, and HR = 0.74 (0.60–0.92), p = 0.006; respectively] when compared to the lowest tertile. Similarly, women with increased levels of physical activity have higher rates of breastfeeding at twelve months, compared to the lowest level [odds ratio OR = 1.71 (95% CI 1.29–2.25) and 1.38 (1.06–1.79)]. Higher levels of physical activity by pregnant women are associated with improved breastfeeding outcomes.
Prevalence and Distribution of Dengue Virus in Aedes aegypti in Yogyakarta City before Deployment of Wolbachia Infected Aedes aegypti
Authors: Rahayu Ayu , Saraswati Utari , Supriyati Endah , Kumalawati Dian Aruni , Hermantara Rio , Rovik Anwar , Daniwijaya Edwin Widyanto , Fitriana Iva , Setyawan Sigit , Ahmad Riris Andono , Wardana Dwi Satria , Indriani Citra , Utarini Adi , Tantowijoyo Warsito , Arguni Eggi
Published: May 2019

Indonesia is one of the countries where dengue infection is prevalent. In this study we measure the prevalence and distribution of dengue virus (DENV) DENV-infected Aedes aegypti in Yogyakarta City, Indonesia, during the wet season when high dengue transmission period occurred, as baseline data before implementation of a Wolbachia-infected Aedes aegypti trial for dengue control. We applied One-Step Multiplex Real Time PCR (RT-PCR) for the type-specific-detection of dengue viruses in field-caught adult Aedes aegypti mosquitoes. In a prospective field study conducted from December 2015 to May 2016, adult female Aedes aegypti were caught from selected areas in Yogyakarta City, and then screened by using RT-PCR. During the survey period, 36 (0.12%) mosquitoes from amongst 29,252 female mosquitoes were positive for a DENV type. In total, 22.20% of dengue-positive mosquitoes were DENV-1, 25% were DENV-2, 17% were DENV-3, but none were positive for DENV-4. This study has provided dengue virus infection prevalence in field-caught Aedes aegypti and its circulating serotype in Yogyakarta City before deployment of Wolbachia-infected Aedes aegypti.
Cashew Tree Pollen: An Unknown Source of IgE-Reactive Molecules
Authors: Danella Figo Daniele , De Amicis Karine , Neiva Santos de Aquino Denise , Pomiecinski Fabiane , Gadermaier Gabriele , Briza Peter , Eduardo Santos Galvão Clovis , Bussador do Amaral Jônatas , de Oliveira Martins Carlo , Fernandes Morato Castro Fabio , Kalil Jorge , Souza Santos Keity
Published: May 2019

Pollinosis is sub-diagnosed and rarely studied in tropical countries. Cashew tree pollen has been reported as an allergen source although the knowledge of its immunoglobulin E (IgE)-reactive molecules is lacking. Therefore, this work aimed to identify IgE-reactive molecules and provide a proteomic profile of this pollen. From the 830 proteins identified by shotgun analysis, 163 were annotated to gene ontology, and a list of 39 proteins filtered for high confidence was submitted to the Allfam database where nine were assigned to allergenic families. Thus, 12 patients from the northeast of Brazil with persistent allergic rhinitis and aggravation of symptoms during cashew flowering season were selected. Using a 2D-based approach, we identified 20 IgE-reactive proteins, four already recognized as allergens, including a homolog of the birch isoflavone-reductase (Bet v 6). IgE-reactivity against the extract in native form was confirmed for five patients in ELISA, with three being positive for Bet v 6. Herein, we present a group of patients with rhinitis exposed to cashew tree pollen with the first description of IgE-binding proteins and a proteomic profile of the whole pollen. Cashew tree pollen is considered an important trigger of rhinitis symptoms in clinical practice in the northeast of Brazil, and the elucidation of its allergenic molecules can improve the diagnostics and treatment for allergic patients.
HER2-Specific Targeted Toxin DARPin-LoPE: Immunogenicity and Antitumor Effect on Intraperitoneal Ovarian Cancer Xenograft Model
Authors: Sokolova Evgeniya A. , Shilova Olga N. , Kiseleva Daria V. , Schulga Alexey A. , Balalaeva Irina V. , Deyev Sergey M.
Published: May 2019

High immunogenicity and systemic toxicity are the main obstacles limiting the clinical use of the therapeutic agents based on Pseudomonas aeruginosa exotoxin A. In this work, we studied the immunogenicity, general toxicity and antitumor effect of the targeted toxin DARPin-LoPE composed of HER2-specific DARPin and a low immunogenic exotoxin A fragment lacking immunodominant human B lymphocyte epitopes. The targeted toxin has been shown to effectively inhibit the growth of HER2-positive human ovarian carcinoma xenografts, while exhibiting low non-specific toxicity and side effects, such as vascular leak syndrome and liver tissue degradation, as well as low immunogenicity, as was shown by specific antibody titer. This represents prospects for its use as an agent for targeted therapy of HER2-positive tumors.
Water Deficit Elicits a Transcriptional Response of Genes Governing d-pinitol Biosynthesis in Soybean (Glycine max)
Authors: Dumschott Kathryn , Dechorgnat Julie , Merchant Andrew
Published: May 2019

d-pinitol is the most commonly accumulated sugar alcohol in the Leguminosae family and has been observed to increase significantly in response to abiotic stress. While previous studies have identified genes involved in d-pinitol synthesis, no study has investigated transcript expression in planta. The present study quantified the expression of several genes involved in d-pinitol synthesis in different plant tissues and investigated the accumulation of d-pinitol, myo-inositol and other metabolites in response to a progressive soil drought in soybean (Glycine max). Expression of myo-inositol 1-phosphate synthase (INPS), the gene responsible for the conversion of glucose-6-phosphate to myo-inositol-1-phosphate, was significantly up regulated in response to a water deficit for the first two sampling weeks. Expression of myo-inositol O-methyl transferase (IMT1), the gene responsible for the conversion of myo-inositol into d-ononitol was only up regulated in stems at sampling week 3. Assessment of metabolites showed significant changes in their concentration in leaves and stems. d-Pinitol concentration was significantly higher in all organs sampled from water deficit plants for all three sampling weeks. In contrast, myo-inositol, had significantly lower concentrations in leaf samples despite up regulation of INPS suggesting the transcriptionally regulated flux of carbon through the myo-inositol pool is important during water deficit.
ATP-Binding Cassette Transporter Regulates N,N′-diacetylchitobiose Transportation and Chitinase Production in Trichoderma asperellum T4
Authors: Liu He , Cheng Ming , Zhao Shanshan , Lin Congyu , Song Jinzhu , Yang Qian
Published: May 2019

ATP-binding cassette (ABC) transporters are a superfamily of proteins that transport nutrient substances and secondary metabolites through cell membranes. They also act as an uptake system for N,N′-diacetylchitobiose (GlcNAc)2 in Streptomyces coelicolor. (GlcNAc)2 is an important inducer of chitinase. However, whether the ABC transporter in Trichoderma spp. is also responsible for (GlcNAc)2 uptake and chitinase induction has not yet been confirmed. In this study, we applied RNA interference and overexpression technologies to alter the expression level of the ABC-B transporter in order to detect changes in its transportation ability and the expression level of inducible endo-chitinase ECH42—an important biocontrol enzyme in Trichoderma asperellum. The results revealed that, after interference with the expression of the ABC-B transporter, T. asperellum T4 was only able to grow normally when glucose was the only carbon source. Compared with the wild-type, the efficiency of (GlcNAc)2 by the overexpression strain evidently increased, along with the activity level of ECH42. In conclusion, one of the functions of the ABC-B transporter in T. asperellum is the uptake and transport of (GlcNAc)2 into cells, and chitobiose is a strong inducer of ECH42 in T. asperellum T4.
Fendiline Enhances the Cytotoxic Effects of Therapeutic Agents on PDAC Cells by Inhibiting Tumor-Promoting Signaling Events: A Potential Strategy to Combat PDAC
Authors: Alhothali Marwa , Mathew Mevin , Iyer Geeta , Lawrence Harshani R. , Yang Shengyu , Chellappan Srikumar , Padmanabhan Jaya
Published: May 2019

The L-type calcium channel blocker fendiline has been shown to interfere with Ras-dependent signaling in K-Ras mutant cancer cells. Earlier studies from our lab had shown that treatment of pancreatic cancer cells with fendiline causes significant cytotoxicity and interferes with proliferation, survival, migration, invasion and anchorage independent growth. Currently there are no effective therapies to manage PDACs. As fendiline has been approved for treatment of patients with angina, we hypothesized that, if proven effective, combinatorial therapies using this agent would be easily translatable to clinic for testing in PDAC patients. Here we tested combinations of fendiline with gemcitabine, visudyne (a YAP1 inhibitor) or tivantinib (ARQ197, a c-Met inhibitor) for their effectiveness in overcoming growth and oncogenic characteristics of PDAC cells. The Hippo pathway component YAP1 has been shown to bypass K-Ras addiction, and allow tumor growth, in a Ras-null mouse model. Similarly, c-Met expression has been associated with poor prognosis and metastasis in PDAC patients. Our results presented here show that combinations of fendiline with these inhibitors show enhanced anti-tumor activity in Panc1, MiaPaCa2 and CD18/HPAF PDAC cells, as evident from the reduced viability, migration, anchorage-independent growth and self-renewal. Biochemical analysis shows that these agents interfere with various signaling cascades such as the activation of Akt and ERK, as well as the expression of c-Myc and CD44 that are altered in PDACs. These results imply that inclusion of fendiline may improve the efficacy of various chemotherapeutic agents that could potentially benefit PDAC patients.
The Impact of Maternal Fructose Exposure on Angiogenic Activity of Endothelial Progenitor Cells and Blood Flow Recovery After Critical Limb Ischemia in Rat Offspring
Authors: Leu Steve , Wu Kay L. H. , Lee Wei-Chia , Tain You-Lin , Chan Julie Y. H.
Published: May 2019

Adult metabolic syndrome is considered to be elicited by the developmental programming which is regulated by the prenatal environment. The maternal excess intake of fructose, a wildly used food additive, is found to be associated with developmental programing-associated cardiovascular diseases. To investigate the effect of maternal fructose exposure (MFE) on endothelial function and repair, which participate in the initiation and progress of cardiovascular disease, we applied a rat model with maternal fructose excess intake during gestational and lactational stage and examined the number and function of endothelial progenitor cells (EPCs) in 3-month-old male offspring with induction of critical limb ischemia (CLI). Results showed that the circulating levels of c-Kit+/CD31+ and Sca-1+/KDR+ EPC were reduced by MFE. In vitro angiogenesis analysis indicated the angiogenic activity of bone marrow-derived EPC, including tube formation and cellular migration, was reduced by MFE. Western blots further indicated the phosphorylated levels of ERK1/2, p38-MAPK, and JNK in circulating peripheral blood mononuclear cells were up-regulated by MFE. Fourteen days after CLI, the reduced blood flow recovery, lowered capillary density, and increased fibrotic area in quadriceps were observed in offspring with MFE. Moreover, the aortic endothelium-mediated vasorelaxant response in offspring was impaired by MFE. In conclusion, maternal fructose intake during gestational and lactational stage modulates the number and angiogenic activity of EPCs and results in poor blood flow recovery after ischemic injury.