bluuebunny/crossref_metadata_2025 huggingface dataset:
La participación y la interdisciplinariedad como elementos de gestión ambiental - educativa para la construcción de lineamientos curriculares de educación ambiental. Estudio de caso Gimnasio Vermont, localidad de Suba
Authors:   , Rojas Sarmiento Juanita
Published: July 2022

El presente artículo hacer referencia al desarrollo de procesos para el establecimiento de proyectos de educación ambiental que exhortan por la resolución, prevención y mitigación de los problemas ambientales, abordados desde el currículo, como son los Proyectos Ambientales Escolares (PRAE). A través de un ejemplo de caso, desarrollado en un colegio privado del norte de Bogotá, se trabajó con los docentes de diferentes áreas del conocimiento en la identificación de los problemas ambientales y en la formulación de lineamientos curriculares para abordarlos.
Análisis de la contribución de los sanitarios secos al saneamiento básico rural : caso : vereda Chorrillos
Authors:   , Montes Ana Karina
Published: July 2022

El trabajo de grado tuvo como propósito hacer un análisis basado en el estudio de caso de la experiencia piloto de los sanitarios secos en la Vereda Chorrillo con el ánimo de despertar el interés sobre el funcionamiento beneficios económicos y sociales de este sistema alternativo. Este sistema se ubica dentro de una propuesta ambientalista que permite el desarrollo sostenible de las zonas rurales del país y de ecosistemas estratégicos donde hay asentamientos humanos. El trabajo establece unos objetivos un recorrido de experiencias con sanitarios secos en el ámbito mundial nacional y distrital posterior a esto se abordan los pasos metodológico en el marco de la investigación cualitativa usando estrategias como el estudio de caso y la observación participante apoyado por técnicas e instrumentos como entrevistas al grupo focal informantes claves y semi-estructuradas a población en general la información recopilada fue interpretada en un matriz de análisis de datos cuantitativos con el fin de realizar la discusión y conclusiones de esta experiencia piloto en la Vereda Chorrillos zona rural de la Localidad de Suba y que esta sea tomada como una experiencia como una experiencia exitosa y replicada en otras zonas con problemáticas socio-ambientales similares.
Propuesta de gestión ambiental para la empresa SIPROCAS EU del sector ganadero en Hato Corozal - Casanare
Authors:   , Espitia Pinto Nelson Enrique
Published: July 2022

SIPROCAS EU es una empresa colombiana que se creó en el año 2008 gracias a la iniciativa de un proyecto empresarial denominado Sistemas Productivos del Casnare "SIPROCAS", el cual fue financiado por el gobierno nacional a través del FONDOEMPRENDER. La empresa se encuentra ubicada en el piedemonte llanero del municipio de Hato Corozal Casanare, exactamente en la Finca ISLANDIA de la vereda de Guayureme, la cual tiene extensión de 130 Ha dividas en Ha de bosque de piedemonte y 70 Ha de praderas con pastos mejorados. La empresa con el fin de mejorar su desempeño ambiental tomo la decisión de adoptar una política ambiental para comprometerse con el uso y manejo adecuado de los recursos naturales, la prevención de la contaminación y el cumplimiento de los requisitos legales que le aplican. De acuerdo con esto se diseño una propuesta de gestión ambiental para orientar la toma de decisiones de la empresa hacia el desarrollo sostenible. La propuesta está compuesta por la política, objetivos, metas y programas ambientales que fueron diseñados bajo los requisitos de la NTC ISO 14001:2004.
Agonistas, sub/alternas y reservadas memorias de las víctimas
Authors:   , Acevedo Arango Óscar Fernando
Published: July 2022

El trabajo Agonistas, sub/alternas y reservadas: memorias de las víctimas presenta un análisis y una propuesta conceptual para comprender algunas de las distintas posiciones que adopta la memoria de las víctimas en Colombia: oficial/gubernativa, agonista, subalternas-sub/alternas, y reservadas. Como tal expone la heterogeneidad en as intenciones y expresiones y la movilidad que experimentan a través del tiempo. En el primer capítulo Lo personal es histórico, lo histórico es personal, el autor expone su memoria autobiográfica y etnográfica del conflicto social que vivenció en la ciudad de Medellín durante las décadas de los ochenta, noventa y principios de este siglo. Bajo la modalidad de lo que ha dado en nombrar una arqueología emocional. En el segundo capítulo Contextualizar las memorias en Colombia: nociones, posiciones y rutas, presenta algunos de los conceptos que delimitan las políticas de la memoria a nivel nacional e internacional y propone el esquema conceptual de memorias gubernativas, agonistas sub/alternas y reservadas. Los capítulos tercero y cuarto Memorias agonistas: poder y posibilidades de los memoriales de las víctimas de la izquierda democrática en Colombia, y Memorias sub/alternas y reservadas: poder y posibilidades de las memorias de otros modos consisten respectivamente en una aplicación analítica de los conceptos propuestos en torno a distintas experiencias de la memoria de las víctimas que se divulgaron en Colombia a partir del año 2005.
Caracterización de patrones mentales en el uso de parábolas literarias en estudiantes de grado noveno de educación básica secundaria
Authors:   , Cortés Andrea , Rodríguez Bogotá Melba , Torres Álvarez Alcira Janneth
Published: July 2022

Esta investigación pretende caracterizar los patrones mentales según la teoría de Turner (planeación, predicción y evaluación)| que se evidencian en parábolas literarias, elaboradas por jóvenes entre 14 y 16 años, de grado noveno de básica secundaria, de acuerdo con el sistema de educación colombiana. Para tal fin, se aplicaron tres pruebas, en las cuales, mediante la técnica de Análisis de Contenido y el software Atlas. ti, se sistematizaron los datos, fue posible evidenciar las características propias de cada patrón mental. La presente investigación ahondó en la parábola literaria, desde dos perspectivas, su estructura narrativa y cognitiva. Además, se abrió la posibilidad a futuras mediaciones educativas.
En los bordes de la escritura mística de la Madre Francisca Josefa de la Concepción del Castillo (1671-1742)
Authors:   , Fonseca Zamora Inés
Published: July 2022

Pretendo un primer desciframiento de la Vida y los Afectos Espirituales de la Madre Francisca Josefa del Castillo (Tunja 1671-1742) en el que busco reconocer el territorio textual simbólico de su escritura mística femenina bajo la mirada del predicadorinquisidor- corrector de su vida y de su obra. Aunque la palabra rompe con el silencio propio del espacio literario de la mística, es ese mismo silencio tras de las palabras el que exige una lectura nueva. Lectura ésta que permite compartir el afecto-efecto de su voz y su polifonía, entre los intersticios del silencio y la escritura de su obra literaria. Es la palabra que nombra y lo que oculta, la que guía la lectura.
Su escritura pretende fijar la palabra que revela y vela, que descubre y esconde, que ilumina y oscurece, que sorprende y des-vela. Así, el silencio como concepto actúa a la manera de ganzúa que abre puertas indebidas, como personaje que interpela insistentemente, valiéndose del movimiento metonímico de los conceptos.
La Madre del Castillo experimentó el goce-dolor de la escritura femenina en la Colonia misógina, el éxtasis de la palabra creadora, las suavidades infinitamente profundas y ciertas del Amor Celeste. Escribió dentro del dogma, caminando intensamente comprometida con la vida. Así, se convirtió en el espejo de la flaqueza humana y en el umbral de partida en la búsqueda del sentido de la vida femenina.
Tomémonos un tinto experiencia de radio y extensión rural del Comité de Cafeteros del Tolima
Authors:   , Castro Chávez Juan Pablo
Published: July 2022

El análisis planteado gira en torno a la experiencia de radio cafetera que desde el Tolima se hace con el programa Tomémonos un Tinto, el cual hace parte integral de la propuesta de comunicación para el desarrollo de la Federación Nacional de cafeteros. Existen, además, elementos en común que permiten establecer e identificar la estrecha relación que surge de la triada: extensión, comunicación y desarrollo| componentes conceptuales del documento y evidenciados en los resultados del trabajo de campo con la comunidad de Risaralda Calarma, en el municipio de Chaparral, sur del Tolima.
Wrongful birth cases en Estados Unidos y España : un análisis jurisprudencial desde la teoría discursiva del derecho de Jürgen Habermas
Authors:   , Bastidas Goyes Luis Guillermo
Published: July 2022

Dado que los avances en materia de diagnóstico preconceptivo, preimplantatorio o prenatal permiten a los padres poder decidir sobre la concepción, implantación o continuidad de un embarazo en curso, las acciones wrongful birth, que se interponen contra médicos que no han desempeñado su labor diligentemente (lex artis ad hoc) al momento de establecer o comunicar diagnósticos con base en los cuales los padres podrían decidir la interrupción del embarazo, han aumentado gradualmente en países como Estados Unidos y España. Sobre la base de la teoría discursiva del derecho de Habermas, según la cual las decisiones judiciales deben basarse en interpretaciones válidas y legítimas de normas debidamente fundamentadas, este trabajo examina la aceptabilidad de dos líneas jurisprudenciales de decisión en materia de wrongful bith cases (en Estados Unidos y España). La principal conclusión del trabajo es que la toma de decisiones jurídicas debería incluir el diálogo interdisciplinario en lugar de dejar a los jueces decidir solos sobre estos asuntos. Una relación entre bioética y derecho parece ser necesaria cuando deben tomarse decisiones normativas sobre la aceptabilidad de acciones que comprometen la vida humana.
Estado de la enseñanza bioética en las facultades de odontología de Bogotá
Authors:   , Echeverri Aponte Luz Adriana , Quintero Mur Leidy
Published: July 2022

Con el objetivo de conocer la enseñanza de la Bioética en las facultades de odontología de Bogotá, se realizó este trabajo el cual analiza la educación de la Bioética en las ciencia de la salud oral, para este estudio se utilizaron categorías de estudio que incluyen los énfasis temáticos de la asignatura entre otros. La estrategia metodológica empleada consistió en la revisión de los planes académicos de las facultades y entrevistas realizadas a los docentes y alumnos de esta asignatura. Dentro de las principales conclusiones esta que la enseñanza de bioética está siendo orientada hacia la normatividad jurídica de la profesión, además de encontrar una confusión entre los conceptos de Ética y Bioética.
La argumentación en el discurso académico en Lengua de Señas Colombiana L.S.C. en el área de biología en educandos sordos de educación media del colegio San Francisco IED jornada mañana de Bogotá
Authors:   , Ballen Castiblanco Beiman Andrey
Published: July 2022

Esta investigación tuvo como propósito indagar qué sucede con la competencia argumentativa que fundamenta el discurso académico en la asignatura de biología en un grupo de 8 educandos sordos, tres niños y cinco niñas, cuyas edades oscilan entre los 12 y los 14 años y se encuentran en el ciclo III en el grado octavo (804). El enfoque que guió la investigación fue la investigación cualitativo, con un método de acción participativa, las técnicas de recolección de la informaron fueron las entrevistas, las observaciones, la reflexión de la práctica del docente investigador a partir de la elaboración de un relato. Una vez se aplicaron los instrumentos durante el trabajo en las temáticas de biología, se realizó el diseño y ejecución de una estrategia pedagógica que permitiera aportar a la transformación de los niveles de argumentación en Lengua de Señas Colombiana LSC, en el área de ciencias naturales, proceso biológico, enfocada en las temáticas de sistema nervioso y de órganos de los sentidos, lo anterior permitió encontrar que la aplicación de dicha estrategia promueve importantes implicaciones en el desarrollo de la competencia argumentativa en el discurso académico en lengua de señas colombiana (LSC) en biología, además de aportar a otros procesos y dimensiones de formación de los educandos Sordos.
Comprensión de metáforas en niños de 8 y 10 años
Authors:   , Aldana Serrano María Carolina
Published: July 2022

Existe poca investigación respecto a lenguaje metafórico y niños. Por el contrario, se ha investigado mucho sobre la comprensión de metáforas en adolescentes y adultos. El presente artículo muestra un trabajo en este campo, abriendo las puertas a la investigación de este fenómeno del lenguaje en niños pequeños. Este trabajo corrobora los planteamientos que Glucksberg había hecho en su Modelo de Inclusión de Clases, sobre metáforas y la comprensión de las mismas. La comprensión de metáforas, y del lenguaje figurado en general, es importante debido a que éstas hacen parte de nuestras formas de comunicación y socialización con otros. En este trabajo se realizaron dos pruebas a niños, sin ningún tipo de intervención, con el fin de determinar si elementos como el contexto, de acuerdo con los planteamientos de Glucksberg, son importantes al momento de comprender un enunciado metafórico.
La misión Capuchina en el Caquetá y Putumayo : 1893-1929
Authors:   , Kuan Bahamón Misael
Published: July 2022

A mediados del siglo XIX, el Estado colombiano vio necesaria la incorporación de los indígenas de territorios de frontera al sistema económico y la civilización de éstos. Los misioneros católicos fueron considerados idóneos para tal empresa. Esta investigación examina las misiones capuchinas en el Caquetá y Putumayo entre 1893 y 1929. El trabajo tiene tres capítulos. El primer capítulo narra los ensayos de incorporación que se dieron desde la Colonia. El segundo capítulo comprende el primer periodo de la misión capuchina (1893-1905). Se estudian la historia de los capuchinos y los primeros métodos misioneros, las tácticas indígenas y las manifestaciones de intercambio cultural. El tercer capítulo se detiene en la Prefectura Apostólica del Caquetá (1905-1929) y en las estrategias de la Misión, las tácticas indígenas y algunos resultados culturales.
The Case for Waste to Energy
Authors: Castaldi Marco J. , LeBlanc Jeff , Licata Anthony
Published: July 2022

There is a better way of handling MSW that is not or cannot be recycled: thermal conversion, also called waste to energy. These facilities feed waste into a combustion chamber with air and incinerate it. The heat released from combustion produces steam for use in a district heat network, or to generate electricity, or do both in combined heat and power systems. In addition to enabling the heat content of the MSW to be recovered, combustion reduces its final volume by more than 90 percent, thus decreasing the need for landfills.
Effect of ultrasound amplitude and frequency on nanoparticle diffusion in an agarose hydrogel
Authors: Karki Alina , Marshall Jeffrey S. , Wu Junru
Published: July 2022

Exposure of nanoparticles in a porous medium, such as a hydrogel, to low-intensity ultrasound has been observed to dramatically enhance particle penetration rate. Enhancement of nanoparticle penetration is a key issue affecting applications such as biofilm mitigation and targeted drug delivery in human tissue. The current study used fluorescent imaging to obtain detailed experimental measurements of the effect of ultrasound amplitude and frequency on diffusion of nanoparticles of different diameters in an agarose hydrogel, which is often used as a simulant for biofilms and biological tissues. We demonstrate that the acoustic enhancement occurs via the phenomenon of oscillatory diffusion, in which a combination of an oscillatory flow together with random hindering of the particles by interaction with hydrogel proteins induces a stochastic random walk of the particles. The measured variation of acoustic diffusion coefficients with amplitude and frequency were used to validate a previous statistical theory of oscillatory diffusion based on the continuous time random walk approach.
Teaching acoustic phonetics to undergraduates in communication sciences and disorders: Course structure and sample projects
Authors: Levi Susannah V.
Published: July 2022

Virtually all undergraduate communication sciences and disorders programs require a course that covers acoustic phonetics. Students typically have a separate phonetics (transcription) course prior to taking the acoustic phonetics course. This paper describes a way to structure an acoustic phonetics course into two halves: a first half that focuses on the source, including basic acoustics (simple harmonic motion, harmonics), vocal fold vibration, modes of phonation, and intonation, and a second half that focuses on the filter, including resonance and tube models, vowel formants, and consonant acoustics. Thus, basic acoustic properties are interwoven with specific examples of speech-related acoustics. In addition, two projects that illustrate concepts from the two halves of the course (one on fundamental frequency and the other on vowel formants) are presented.
Intravital calcium imaging in myeloid leukocytes identifies calcium frequency spectra as indicators of functional states
Authors: Mehari Fitsumbirhan T. , Miller Meike , Pick Robert , Bader Almke , Pekayvaz Kami , Napoli Matteo , Uhl Bernd , Reichel Christoph A. , Sperandio Markus , Walzog Barbara , Schulz Christian , Massberg Steffen , Stark Konstantin
Published: July 2022

The assessment of leukocyte activation in vivo is mainly based on surrogate parameters, such as cell shape changes and migration patterns. Consequently, additional parameters are required to dissect the complex spatiotemporal activation of leukocytes during inflammation. Here, we showed that intravital microscopy of myeloid leukocyte Ca2+signals with Ca2+reporter mouse strains combined with bioinformatic signal analysis provided a tool to assess their activation in vivo. We demonstrated by two-photon microscopy that tissue-resident macrophages reacted to sterile inflammation in the cremaster muscle with Ca2+transients in a distinct spatiotemporal pattern. Moreover, through high-resolution, intravital spinning disk confocal microscopy, we identified the intracellular Ca2+signaling patterns of neutrophils during the migration cascade in vivo. These patterns were modulated by the Ca2+channel Orai1 and Gαi-coupled GPCRs, whose effects were evident through analysis of the range of frequencies of the Ca2+signal (frequency spectra), which provided insights into the complex patterns of leukocyte Ca2+oscillations. Together, these findings establish Ca2+frequency spectra as an additional dimension to assess leukocyte activation and migration during inflammation in vivo.
CRISPR–Cas9 Based Bacteriophage Genome Editing
Authors: Zhang Xueli , Zhang Chaohui , Liang Caijiao , Li Bizhou , Meng Fanmei , Ai Yuncan
Published: July 2022

Bacteriophages are the most numerous organisms on earth and are known for their diverse lifestyles. Since the discovery of bacteriophages, our knowledge of the wider biological world has undergone immense and unforeseen changes.
Novel Insights into the Pig Gut Microbiome Using Metagenome-Assembled Genomes
Authors: Holman Devin B. , Kommadath Arun , Tingley Jeffrey P. , Abbott D. Wade
Published: July 2022

Many of the bacterial strains found in the mammalian gut are difficult to culture and isolate due to their various growth and nutrient requirements that are frequently unknown. Here, we assembled strain-level genomes from short metagenomic sequences, so-called metagenome-assembled genomes (MAGs), that were derived from fecal samples collected from pigs at multiple time points.
Interleukin 32 as a Potential Marker for Diagnosis of Tuberculous Pleural Effusion
Authors: Du Juan , Shao Ming-Ming , Yi Feng-Shuang , Huang Zhong-Yin , Qiao Xin , Chen Qing-Yu , Shi Huan-Zhong , Zhai Kan
Published: July 2022

Tuberculous pleural effusion (TPE) is a common form of extrapulmonary tuberculosis, with manifestations ranging from benign effusion with spontaneous absorption to effusion with pleural thickening, empyema, and even fibrosis, which can lead to a lasting impairment of lung function. Therefore, it is of great significance to find a rapid method to establish early diagnosis and apply antituberculosis therapy in the early stage.
Honeycomb epithelial oedema due to ripasudil: clinical, optical coherence tomography and histopathological correlation
Authors: Jain Neha , Singh Archana , Mishra Dilip K , Murthy Somasheila I
Published: July 2022

A male in his 50s, who had undergone Descemet’s stripping automated endothelial keratoplasty (DSAEK) for corneal oedema following irido-corneal-endothelial syndrome and glaucoma drainage device surgery for secondary glaucoma presented with gradual decreased vision. The patient was on ripasudil 0.4% for the past 4 months prior to the presentation. Honeycomb epithelial oedema was seen with vesicles and intraepithelial microcyst formation which were also noted on anterior segment optical coherence tomography (OCT) and histopathology, that co-related with the slit-lamp findings. The patient underwent repeat DSAEK and had a good outcome. While the clinical features and OCT findings have been reported earlier, the histopathological features have not been reported.
Living through the psychological consequences of COVID-19 pandemic: a systematic review of effective mitigating interventions
Authors: Lekagul Angkana , Piancharoen Peeraya , Chattong Anamika , Suradom Chawisa , Tangcharoensathien Viroj
Published: July 2022

Objective

This review assesses interventions and their effectiveness in mitigating psychological consequences from pandemic.

Method

Published English literatures were searched from four databases (Medline, PubMed, Embase and PsycINFO) from January 2020 and September 2021. A total of 27 papers with 29 studies (one paper reported three studies) met inclusion criteria. Cochrane risk-of-bias tool is applied to assess the quality of all randomised controlled trials (RCT).

Results

All studies were recently conducted in 2020. Publications were from high-income (13, 44.8%), upper middle-income (12, 41.4%) and lower middle-income countries (3, 10.3%) and global (1, 3.5%). Half of the studies conducted for general population (51.7%). One-third of studies (8, 27.6%) provided interventions to patients with COVID-19 and 20.7% to healthcare workers. Of the 29 studies, 14 (48.3%) were RCT. All RCTs were assessed for risk of biases; five studies (15, 35.7%) had low risk as measured against all six dimensions reflecting high-quality study.

Of these 29 studies, 26 diagnostic or screening measures were applied; 8 (30.9%) for anxiety, 7 (26.9%) for depression, 5 (19.2%) for stress, 5 (19.2%) for insomnia and 1 (3.8%) for suicide. Measures used to assess the baseline and outcomes of interventions were standardised and widely applied by other studies with high level of reliability and validity. Of 11 RCT studies, 10 (90.9%) showed that anxiety interventions significantly lowered anxiety in intervention groups. Five of the six RCT studies (83.3%) had significantly reduced the level of depression. Most interventions for anxiety and stress were mindfulness and meditation based.

Conclusions

Results from RCT studies (11%, 78.6%) were effective in mitigating psychological consequences from COVID-19 pandemic when applied to healthcare workers, patients with COVID-19 and general population. These effective interventions can be applied and scaled up in other country settings through adaptation of modes of delivery suitable to country resources, pandemic and health system context.
Digital multiple health behaviour change intervention targeting online help seekers: protocol for the COACH randomised factorial trial
Authors: Åsberg Katarina , Blomqvist Jenny , Lundgren Oskar , Henriksson Hanna , Henriksson Pontus , Bendtsen Preben , Löf Marie , Bendtsen Marcus
Published: July 2022

Introduction

Unhealthy lifestyle behaviours continue to be highly prevalent, including alcohol consumption, unhealthy diets, insufficient physical activity and smoking. There is a lack of effective interventions which have a large enough reach into the community to improve public health. Additionally, the common co-occurrence of multiple unhealthy behaviours demands investigation of efforts which address more than single behaviours.

Methods and analysis

The effects of six components of a novel digital multiple health behaviour change intervention on alcohol consumption, diet, physical activity and smoking (coprimary outcomes) will be estimated in a factorial randomised trial. The components are designed to facilitate behaviour change, for example, through goal setting or increasing motivation, and are either present or absent depending on allocation (ie, six factors with two levels each). The study population will be those seeking help online, recruited through search engines, social media and lifestyle-related websites. Included will be those who are at least 18 years of age and have at least one unhealthy behaviour. An adaptive design will be used to periodically make decisions to continue or stop recruitment, with simulations suggesting a final sample size between 1500 and 2500 participants. Multilevel regression models will be used to analyse behavioural outcomes collected at 2 months and 4 months postrandomisation.

Ethics and dissemination

Approved by the Swedish Ethical Review Authority on 2021-08-11 (Dnr 2021-02855). Since participation is likely motivated by gaining access to novel support, the main concern is demotivation and opportunity cost if the intervention is found to only exert small effects. Recruitment began on 19 October 2021, with an anticipated recruitment period of 12 months.

Trial registration number

ISRCTN16420548.
CRISPR-mediated TGFBR2 knockout renders human ovarian cancer tumor-infiltrating lymphocytes resistant to TGF-β signaling
Authors: Fix Samantha M , Forget Marie-Andrée , Sakellariou-Thompson Donastas , Wang Yunfei , Griffiths Tamara M , Lee Minjung , Haymaker Cara L , Dominguez Ana Lucía , Basar Rafet , Reyes Christopher , Kumar Sanjay , Meyer Larissa A , Hwu Patrick , Bernatchez Chantale , Jazaeri Amir A
Published: July 2022

Background

The correlation between elevated T-cell infiltration and improved survival of ovarian cancer (OvCa) patients suggests that endogenous tumor-infiltrating lymphocytes (TIL) possess some degree of antitumor activity that can be harnessed for OvCa immunotherapy. We previously optimized a protocol for ex vivo OvCa TIL expansion for adoptive cell therapy, which is now being tested in a clinical trial at our institution (NCT03610490). Building on this success, we embarked on genetic modification of OvCa TIL to overcome key immunosuppressive factors present in the tumor microenvironment. Here, we present the preclinical optimization of CRISPR/Cas9-mediated knockout of the TGF-β receptor 2 (TGFBR2) in patient-derived OvCa TIL.

Methods

OvCa TILs were generated from four patients’ tumor samples obtained at surgical resection and subjected to CRISPR/Cas9-mediated knockout of TGFBR2 before undergoing a rapid expansion protocol. TGFBR2-directed gRNAs were comprehensively evaluated for their TGFBR2 knockout efficiency and off-target activity. Furthermore, the impact of TGFBR2 knockout on TIL expansion, function, and downstream signaling was assayed.

Results

TGFBR2 knockout efficiencies ranging from 59±6% to 100%±0% were achieved using 5 gRNAs tested in four independent OvCa TIL samples. TGFBR2 knockout TIL were resistant to immunosuppressive TGF-β signaling as evidenced by a lack of SMAD phosphorylation, a lack of global transcriptional changes in response to TGF-β stimulation, equally strong secretion of proinflammatory cytokines in the presence and absence of TGF-β, and improved cytotoxicity in the presence of TGF-β. CRISPR-modification itself did not alter the ex vivo expansion efficiency, immunophenotype, nor the TCR clonal diversity of OvCa TIL. Importantly for clinical translation, comprehensive analysis of CRISPR off-target effects revealed no evidence of off-target activity for our top two TGFBR2-targeting gRNAs.

Conclusions

CRISPR/Cas9-mediated gene knockout is feasible and efficient in patient-derived OvCa TIL using clinically-scalable methods. We achieved efficient and specific TGFBR2 knockout, yielding an expanded OvCa TIL product that was resistant to the immunosuppressive effects of TGF-β. This study lays the groundwork for clinical translation of CRISPR-modified TIL, providing opportunities for engineering more potent TIL therapies not only for OvCa treatment, but for the treatment of other solid cancers as well.
Acute pain consult and management is associated with improved mortality in rib fracture patients
Authors: Sborov Katherine D , Dennis Bradley M , de Oliveira Filho Getúlio Rodrigues , Bellister Seth A , Statzer Nicholas , Stonko David P , Guyer Richard A , Wanderer Jonathan P , Beyene Robel T , McEvoy Matthew D , Allen Brian Frazer Scott
Published: July 2022

Introduction

Traumatic rib fractures result in significant patient morbidity and mortality, which increases with patient age and number of rib fractures. A dedicated acute pain service (APS) providing expertize in multimodal pain management may reduce these risks and improve outcomes. We aimed to test the hypothesis that protocolized APS consultation decreases mortality and morbidity in traumatic rib fracture patients.

Methods

This is a retrospective observational, propensity-matched cohort study of adult patients with trauma with rib fractures from 2012 to 2015, at a single, large level 1 trauma center corresponding to introduction and incorporation of APS consultation into the institutional rib fracture pathway. Using electronic medical records and trauma registry data, we identified adult patients presenting with traumatic rib fractures. Patients with hospital length of stay (LOS) ≥2 days were split into two cohorts based on presence of APS consult using 1:1 propensity matching of age, gender, comorbidities and injury severity. The primary outcome was difference in hospital mortality. Secondary outcomes included LOS and pulmonary morbidity.

Results

2486 patients were identified, with a final matched cohort of 621 patients receiving APS consult and 621 control patients. The mortality rate was 1.8% among consult patients and 6.6% among control patients (adjusted OR 0.25, 95% CI 0.13 to 0.50; p=0.001). The average treatment effect of consult on mortality was 4.8% (95% CI 1.2% to 8.5%;. p<0.001). APS consultation was associated with increased intensive care unit (ICU) LOS (1.19 day; 95% CI 0.48 to 1.90; p=0.001) and hospital LOS (1.61 days; 95% CI 0.81 to 2.41 days; p<0.001). No difference in pulmonary complications was observed.

Discussion

An APS consult in rib fracture patients is associated with decreased mortality and no difference in pulmonary complications yet increased ICU and hospital LOS.
Geology, geochemistry, and apatite/titanite U–Pb geochronology of ca. 1.88 Ga alkaline ultrabasic dykes in the Southern Province near Sudbury, Ontario
Authors: Kawohl Alexander , Frimmel Hartwig E. , Whymark Wesley E. , Millonig Leo J. , Gerdes Axel
Published: July 2022

The area northeast of Sudbury, Ontario, is well known for hosting one of the largest unexplained geophysical anomalies in the Canadian Shield, the Temagami Anomaly. In search of a geological explanation for this anomaly, low-grade metamorphic ultrabasic dykes have been discovered in the overlying Huronian Supergroup sedimentary rocks, both in outcrop and in a deep drill core. Here, we report on the first geochemical and geochronological data obtained on these dykes and compare these data with known magmatic units in and around the 1850 Ma impact-generated Sudbury Igneous Complex (SIC). The NW-striking dykes, which cut across sedimentary rocks of the ca. 2.3 Ga Cobalt Group, and which are, in turn, crosscut by pseudotachylitic breccia, are characterized by distinctively high concentrations of Ti, P, Nb, and Zr, highly fractionated rare earth element patterns (La/YbN 7.6–15.5), and a lack of crustal contamination (Nb/Th > 10). Such features are typical of modern ocean island basalts but very different from Palaeoproterozoic rocks previously documented in the wider region. Multigrain U–Pb laser ablation inductively coupled plasma mass spectrometry analyses performed on magmatic titanite and apatite with high Th/U ratios yielded 1876.0 ± 8.7 and 1880.9 ± 8.3 Ma, respectively, which we interpret as the intrusion age of the dykes. This interpretation is supported by similar whole-rock Sm-Nd model ages of 1890–2000 Ma (initial εNd +2.5). This magmatic event in the footwall of the SIC shortly before the impact was coeval with, and likely genetically related to, the 1.88–1.87 Ga Circum-Superior Large Igneous Province.
A description of charmonia decays $$J/\psi \rightarrow B\bar{B}$$ within the QCD factorisation framework
Authors: Kivel Nikolay
Published: July 2022

Decays $$J/\psi \rightarrow B{\bar{B}}$$

J
/
ψ
→
B

B

¯

into octet baryon-antibaryons pairs are studied within the QCD factorisation framework. The next-to-leading power correction to the leading-order decay amplitude is calculated for the first time. The phenomenological analysis also includes the interference with the electromagnetic decay $$J/\psi \rightarrow \gamma ^*\rightarrow B{\bar{B}}$$

J
/
ψ
→

γ
∗

→
B

B

¯

. The branching fractions and the angular coefficients $$\alpha _B$$

α
B

are obtained with an accuracy of about $$20\%$$

20
%

. The obtained results allow us to conclude that the calculated amplitudes provide a dominant contribution and therefore QCD factorisation provides a suitable basis for understanding of these decays.
Delta-resonances and hyperons in proto-neutron stars and merger remnants
Authors: Sedrakian Armen , Harutyunyan Arus
Published: July 2022

The equation of state (EoS) and composition of dense and hot $$\varDelta $$
Δ
-resonance admixed hypernuclear matter is studied under conditions that are characteristic of neutron star binary merger remnants and supernovas. The cold, neutrino free regime is also considered as a reference for the astrophysical constraints on the EoS of dense matter. Our formalism uses the covariant density functional (CDF) theory successfully adapted to include the full $$J^P=1/2^+$$

J
P

=
1
/

2
+

baryon octet and non-strange members of $$J^P=3/2^+$$

J
P

=
3
/

2
+

decouplet with density-dependent couplings that have been suitably adjusted to the existing laboratory and astrophysical data. The effect of $$\varDelta $$
Δ
-resonances at finite temperatures is to soften the EoS of hypernuclear matter at intermediate densities and stiffen it at high densities. At low temperatures, the heavy baryons $$\varLambda $$
Λ
, $$\varDelta ^-$$

Δ
-

, $$\varXi ^-$$

Ξ
-

, $$\varXi ^0$$

Ξ
0

and $$\varDelta ^0$$

Δ
0

appear in the given order if the $$\varDelta $$
Δ
-meson couplings are close to those for the nucleon-meson couplings. As is the case for hyperons, the thresholds of $$\varDelta $$
Δ
-resonances move to lower densities with the increase of temperature indicating a significant fraction of $$\varDelta $$
Δ
’s in the low-density subnuclear regime. We find that the $$\varDelta $$
Δ
-resonances comprise a significant fraction of baryonic matter, of the order of $$10\%$$

10
%

at temperatures of the order of several tens of MeV in the neutrino-trapped regime and, thus, may affect the supernova and binary neutron star dynamics by providing, for example, a new source for neutrino opacity or a new channel for bulk viscosity via the direct Urca processes. The mass-radius relation of isentropic static, spherically symmetric hot compact stars is discussed.
Optical soliton perturbation with fractional temporal evolution having cubic and power law nonlinearities
Authors: Yogita  , Dahiya Sunita , Kumar Hitender , Malik Anand , Gautam Manjeet Singh
Published: July 2022

With an increasing demand for larger bandwidth and solving the problem of internet bottleneck, the formation of solitons with fractional temporal evolution has prompted substantial attention in the field of optics. In this paper, we have used three methods namely, the new mapping method, the unified Riccati equation method, and the new modified sub-ODE method to construct the new soliton solutions to the perturbed nonlinear Schrödinger equation (NLSE) with fractional temporal evolution. Two types of nonlinearities are considered namely, the Kerr law and the power law for all the three utilized methods. Existence criteria for all soliton solutions are presented. We provide two- and three-dimensional graphical illustrations of some acquired solutions. This work also proves the reliability, power, and effectiveness of these new analytical techniques for solving other nonlinear fractional differential equations.
Dynamic First Access Isolation Cache to Eliminate Reuse-Based Cache Side Channel Attacks
Authors: Wang Chong , Yu Hong , Wei Shuai , Song Ke
Published: July 2022

Cache side channel attacks have been used to extract users’ sensitive information such as cryptographic keys. In particular, the reuse-based cache side channel attacks exploit the shared code or data between the attacker and the victim, which can steal the secret with high speed and precision. It has threatened not only the host level but also the cloud level severely. Previous defensive measures are either not flexible enough, or cause a high performance or storage overhead. In this work, we present a dynamic first access isolation cache that eliminates reuse-based cache side channel attacks by providing fine grained first access isolation to overcome these problems. First of all, there are [Formula: see text] bits in each cache line to record the access information and prevent the first time cache hit state brought by the victim from being utilized by the attacker while keeping data shared. Second, we use hierarchy security levels and domains to achieve flexible one way isolation between different domains, and the domains can be a group of processes, a single process, or even a fraction of code. Finally, the monitoring-driven dynamic scheduling mechanism can change the level of a domain at run time, which improves the robustness of this new design. The solution works at all the cache levels and defends against attackers running both on local and cloud. Our implementation in the Zsim simulator demonstrates that the performance overhead for standard performance evaluation corporation 2017 is less than 0.1%, and 0.21% for the multi-thread benchmarks. It performs better than the original first time miss design because of the one way isolation in our design. The only hardware modification is the [Formula: see text] bits per cache line, and several security registers per hardware context, which only brings 3.71% storage overhead.
An alignment-independent three-dimensional quantitative structure–activity relationship study on ron receptor tyrosine kinase inhibitors
Authors: Zarei Omid , Raeppel Stéphane L. , Hamzeh-Mivehroud Maryam
Published: July 2022

Recepteur d’Origine Nantais known as RON is a member of the receptor tyrosine kinase (RTK) superfamily which has recently gained increasing attention as cancer target for therapeutic intervention. The aim of this work was to perform an alignment-independent three-dimensional quantitative structure–activity relationship (3D QSAR) study for a series of RON inhibitors. A 3D QSAR model based on GRid-INdependent Descriptors (GRIND) methodology was generated using a set of 19 compounds with RON inhibitory activities. The generated 3D QSAR model revealed the main structural features important in the potency of RON inhibitors. The results obtained from the presented study can be used in lead optimization projects for designing of novel compounds where inhibition of RON is needed.
Gravitational lensing for power-Maxwell charged quintessence black hole in Rastall gravity
Authors: Molla Niyaz Uddin , Debnath Ujjal
Published: July 2022

In this paper, we investigate the gravitational lensing for power-Maxwell charged quintessence black hole (BH) without cosmological constant in Rastall’s theory of gravity in both the strong-field limit and weak-field limit. Furthermore, we discuss the gravitational lensing by the linear electrodynamic (LE) and nonlinear electrodynamic (NLE) Maxwell field with the equation of state (EoS) parameter of the quintessence ([Formula: see text]) and compared to the case with Einstein’s gravity. We discuss the behaviors of the photon sphere radius [Formula: see text], impact parameter [Formula: see text], strong lensing coefficients [Formula: see text], [Formula: see text] and the deflection angle [Formula: see text] on the equatorial plane [Formula: see text] with the different values of Rastall parameter [Formula: see text] for the case of LE and NLE Maxwell field in the strong field limit. We also investigate the angular separation [Formula: see text], angular position [Formula: see text] and the magnification of the relativistic images. Further, we also study about the weak lensing BHs in Rastall gravity. We investigate the behaviors of weak deflection angle with the different values of Rastall parameter [Formula: see text] for the case of LE and NLE Maxwell field in the weak-field limit.
Shale Crack Identification Based on Acoustic Emission Experiment and Wavenet Data Recovery
Authors: Ye Wenjing , Zhang Fengyi , Wang Lihua , Song Xuehang
Published: July 2022

This work provides a general analysis method for the crack detection in shales. The acoustic emission (AE) testing with data recovery is proposed for determining the crack modes and positions in the test and analysis process of the shale fracturing experiment. A fracturing and in situ AE monitoring system is constructed to collect the experimental data in at least six channels for the crack detection, and the source positions output from AE testing represent the positions of cracks. Due to some uncontrollable reasons, such as the poor coupling between sensors and sample and the sudden failure of the sensors, some parts of experimental data are missing during the experiments. Therefore, a data recovery neural network algorithm based on wavenet model is introduced to reconstruct the missing parts of experimental data in the waveforms. Since the accuracy of data recovery is not satisfying based on the collected experimental data, the interpolation of experimental data is performed to refine the data which can obviously improve the accuracy of data recovery. After all the required experimental data have been recovered, the crack mode for each crack can be determined based on the moment tensor analysis. This analysis method can be extensively applied to the shale crack detection.
Divisibility of reduction in groups of rational numbers II
Authors: Abdullah H. O. , Mustafa A. Ali , Pappalardi F.
Published: July 2022

Given a finitely generated multiplicative group of rational numbers, we compute the density of the set of primes for which the order of the reduction group is divisible by a given integer. Earlier work considers the case of groups of positive rationals. Here, we consider the general case. In the second part of the paper, we test our formulas with several tables.
Multivariate analysis of serum surface-enhanced Raman spectroscopy of liver cancer patients
Authors: Wang Qiwen , Wang Sen , Cui Shengdong , Yang Deyuan , Huang Zheng , Xie Shusen
Published: July 2022

Early diagnosis of liver cancer plays a significant role in reducing its high mortality. In this preliminary study, the feasibility of using serum surface-enhanced Raman spectroscopy (SERS) to identify liver cancer was studied. Serum samples were obtained from liver cancer patients and healthy controls. The differences between the SERS spectra of pre-operation and post-operation of liver cancer patients were also analyzed. The general shape and trend of SERS spectra of health control and liver cancer patients were similar. Multivariate analysis, e.g., PLS-SVM, might be useful for the discrimination of serum SERS spectra of pre-operation and post-operation.
Computer-assisted Analysis of Field Recordings: A Case Study of Georgian Funeral Songs
Authors: Rosenzweig Sebastian , Scherbaum Frank , Müller Meinard
Published: July 2022

Three-voiced funeral songs from Svaneti in North-West Georgia (also referred to as Zär) are believed to represent one of Georgia’s oldest preserved forms of collective music-making. Throughout a Zär performance, the singers often jointly and intentionally drift upwards in pitch. Furthermore, the singers tend to use pitch slides at the beginning and end of sung notes. Musicological studies on tonal analysis or transcription have to account for such musical peculiarities, e.g., by compensating for pitch drifts or identifying stable note events (located between pitch slides). These tasks typically require labor-intensive annotation processes with manual corrections executed by experts with domain knowledge. For instance, in the context of a previous musicological study on pitch inventories (or pitch-class histograms) of Zär performances, ethnomusicologists tediously annotated fundamental frequency (F0) trajectories, stable note events, and pitch drifts for a set of 11 multitrack field recordings. In this article, we study how musicological studies on field recordings can benefit from interactive computational tools that support such annotation processes. As one contribution of this article, we compile a dataset from the previously annotated audio material, which we release under an open-source license for research purposes. As a second contribution, we introduce two computational tools for removing pitch slides and compensating pitch drifts in performances. Our tools were developed in close collaboration with ethnomusicologists and allow for incorporating domain knowledge (e.g., on singing styles or musically relevant harmonic intervals) in the different processing steps. In a case study using our Zär dataset, we evaluate our tools by reproducing the pitch inventories from the original musicological study and subsequently discuss the potential of computer-assisted approaches for interdisciplinary research.
Multigroup Analysis in Information Systems Research using PLS-PM
Authors: Klesel Michael , Schuberth Florian , Niehaves Björn , Henseler Jörg
Published: July 2022

Heterogeneity is a pertinent issue in Information Systems (IS) research because human behavior often differs across groups. In the partial least squares path modeling (PLS-PM) context, several approaches have been proposed to investigate potential group differences. Despite the availability of numerous approaches, literature that compares their efficacy is sparse. Consequently, IS researchers lack guidance on which approach is best suited to detect group differences. We address this issue by presenting the results of an extensive Monte Carlo simulation study that juxtaposes the various approaches' behavior under numerous conditions. In doing so, we first provide an overview on existing approaches proposed for multigroup analysis (MGA) in the PLS-PM context. Moreover, we derive important implications for applied research: Firstly, we show that the omnibus test of group differences (OTG) and approaches based on the comparison of confidence intervals are not recommendable for MGA. Secondly, we provide detailed information as to which approaches are suitable for comparing one specific path coefficient and which are recommended if the complete structural model is compared across groups. Finally, we show that approaches which are designed to compare a single parameter require an adjustment for multiple comparisons when used to compare more than two groups.
African American Archaeology, for Now
Authors: Agbe-Davies Anna S.
Published: July 2022

A focus on institutions frames this examination of the archaeology of African America. While initially emphasizing the institution of slavery and theories of Black difference, the field today has a much wider scope. Researchers engaged in this work critically examine past and present-day institutions. As such, this review also considers the place of African American archaeology in engaged scholarship, critical theory, and self-reflexive practice. As in past reviews, the emphasis is on the United States, with occasional references to important work in the rest of the African diaspora. African American archaeology is shown to be inextricably interwoven with scholarly work in North American archaeology, African American studies, heritage studies, and social theory.