bluuebunny/crossref_metadata_2025 huggingface dataset:
Autologous Gradient Formation under Differential Interstitial Fluid Flow Environments
Authors: Stine Caleb A. , Munson Jennifer M.
Published: January 2022

Fluid flow and chemokine gradients play a large part in not only regulating homeostatic processes in the brain, but also in pathologic conditions by directing cell migration. Tumor cells in particular are superior at invading into the brain resulting in tumor recurrence. One mechanism that governs cellular invasion is autologous chemotaxis, whereby pericellular chemokine gradients form due to interstitial fluid flow (IFF) leading cells to migrate up the gradient. Glioma cells have been shown to specifically use CXCL12 to increase their invasion under heightened interstitial flow. Computational modeling of this gradient offers better insight into the extent of its development around single cells, yet very few conditions have been modelled. In this paper, a computational model is developed to investigate how a CXCL12 gradient may form around a tumor cell and what conditions are necessary to affect its formation. Through finite element analysis using COMSOL and coupled convection-diffusion/mass transport equations, we show that velocity (IFF magnitude) has the largest parametric effect on gradient formation, multidirectional fluid flow causes gradient formation in the direction of the resultant which is governed by IFF magnitude, common treatments and flow patterns have a spatiotemporal effect on pericellular gradients, exogenous background concentrations can abrogate the autologous effect depending on how close the cell is to the source, that there is a minimum distance away from the tumor border required for a single cell to establish an autologous gradient, and finally that the development of a gradient formation is highly dependent on specific cell morphology.
High-Sensitivity PtSe2 Surface Plasmon Resonance Biosensor Based on Metal-Si-Metal Waveguide Structure
Authors: Lin Zhitao , Shu Yiqing , Chen Weicheng , Zhao Yang , Li Jianqing
Published: January 2022

PtSe2 as a novel TMDCs material is used to modify the traditional SPR biosensors to improve the performance. On this basis, this research proposes a metal-Si-metal waveguide structure to further improve the performance of the biosensor. In this study, we not only studied the effects of waveguide structures containing different metals on the performance of biosensor, but also discussed the performance change of the biosensor with the change of PtSe2 thickness. After the final optimization, a BK7-Au-Si-Au-PtSe2 (2 nm) biosensor structure achieved the highest sensitivity of 193.8°/RIU. This work provides a new development idea for the study of SPR biosensors with waveguide structures in the future.
The Impact of Quarantine on Pain Sensation among the General Population in China during the COVID-19 Pandemic
Authors: Sun Jie , Zheng Yong-Bo , Liu Lin , Li Shui-Qing , Zhao Yi-Miao , Zhu Xi-Mei , Que Jian-Yu , Li Ming-Zhe , Liu Wei-Jian , Yuan Kai , Yan Wei , Liu Xiao-Guang , Chang Su-Hua , Chen Xuan , Gao Nan , Shi Jie , Bao Yan-Ping , Lu Lin
Published: January 2022

During the pandemic era, quarantines might potentially have negative effects and disproportionately exacerbate health condition problems. We conducted this cross-sectional, national study to ascertain the prevalence of constant pain symptoms and how quarantines impacted the pain symptoms and identify the factors associated with constant pain to further guide reducing the prevalence of chronic pain for vulnerable people under the pandemic. The sociodemographic data, quarantine conditions, mental health situations and pain symptoms of the general population were collected. After adjusting for potential confounders, long-term quarantine (≥15 days) exposures were associated with an increased risk of constant pain complaints compared to those not under a quarantine (Odds Ratio (OR): 1.26; 95% Confidence Interval (CI): 1.03, 1.54; p = 0.026). Risk factors including unemployment (OR: 1.55), chronic disease history (OR: 2.38) and infection with COVID-19 (OR: 2.15), and any of mental health symptoms including depression, anxiety, insomnia and PTSD (OR: 5.44) were identified by a multivariable logistic regression. Additionally, mediation analysis revealed that the effects of the quarantine duration on pain symptoms were mediated by mental health symptoms (indirect effects: 0.075, p < 0.001). These results advocated that long-term quarantine measures were associated with an increased risk of experiencing pain, especially for vulnerable groups with COVID-19 infection and with mental health symptoms. The findings also suggest that reducing mental distress during the pandemic might contribute to reducing the burden of pain symptoms and prioritizing interventions for those experiencing a long-term quarantine.
Seismic Strengthening of RC Structures Using Wall-Type Kagome Damping System
Authors: Hur Moo-Won , Lee Yonghun , Jeon Min-Jun , Lee Sang-Hyun
Published: January 2022

In this study, the Kagome truss damper, a metallic wire structures, was introduced and its mechanical properties were investigated through theoretical analyses and experimental tests. The yield strength of the Kagome damper is dependent on the geometric shape and diameter of the metallic wire. The Kagome damper has higher resistance to plastic buckling as well as lower anisotropy. Cyclic shear loading tests were conducted to investigate the energy dissipation capacity and stiffness/strength degradation by repeated loadings. The hysteretic properties obtained from the tests suggest that a modification of the ideal truss model with a hinged connection could be used to predict the yield strength and stiffness of the damper. For seismic retrofitting of a low-rise RC moment frame system, a wall-type Kagome damping system (WKDS) was proposed. The effectiveness of the proposed system was verified by conducting cyclic loading tests using a RC frame with/without the WKDS (story drift ratio limit 1.0%). The test results indicated that both the strength and stiffness of the RC frame increased to the target level and that its energy dissipation capacity was significantly enhanced. Nonlinear static and dynamic analyses were carried out to validate that the existing building structure can be effectively retrofitted using the proposed WKDS.
Effect of Silica Fume and Polyvinyl Alcohol Fiber on Mechanical Properties and Frost Resistance of Concrete
Authors: Tan Yan , Xu Ziling , Liu Zeli , Jiang Jiuhong
Published: January 2022

To improve the mechanical properties and frost resistance of concrete, silica fume, and polyvinyl alcohol fiber compounded in concrete. The mechanical and frost resistance of concrete were comprehensively analyzed and evaluated for strength change, mass loss, and relative dynamic elastic modulus change by compressive strength test, flexural strength test, and rapid freeze-thaw test. The results showed that with the incorporation of silica fume and polyvinyl alcohol fiber, the compressive and flexural strengths of concrete were improved, and the decrease in mass loss rate and relative dynamic elastic modulus of concrete after freeze-thaw cycles were significantly reduced, which indicated that the compounding of silica fume and polyvinyl alcohol fiber improved the frost resistance of concrete. When the content of silica fume was 10% and the volume content of polyvinyl alcohol fiber was 1%, the comprehensive mechanical performance and frost resistance of concrete is the best. The compressive strength increased by 26.6% and flexural strength increased by 29.17% compared to ordinary concrete. Based on the test data, to study the macroscopic damage evolution of concrete compound silica fume and polyvinyl alcohol fiber under repeated freeze-thaw conditions. The Weibull distribution probability model and GM (1, 1) model were established. The average relative errors between the predicted and actual data of the two models are small and very close. It is shown that both models can reflect well the development of concrete damage under a freeze-thaw environment. This provides an important reference value and theoretical basis for the durability evaluation and life prediction of compound silica fume and polyvinyl alcohol fiber concrete in cold regions.
Neuropsychiatric Disorders and Frailty in Older Adults over the Spectrum of Cancer: A Narrative Review
Authors: Muzyka Mariya , Tagliafico Luca , Serafini Gianluca , Baiardini Ilaria , Braido Fulvio , Nencioni Alessio , Monacelli Fiammetta
Published: January 2022

Background: The interplay between different neuropsychiatric conditions, beyond dementia, in the presence of a diagnosis of cancer in older adults may mediate patients’ fitness and cancer-related outcomes. Here, we aimed to investigate the presence of depression, sleep disturbances, anxiety, attitude, motivation, and support in older adults receiving a diagnosis of cancer and the dimension of frailty in order to understand the magnitude of the problem. Methods: This review provides an update of the state of the art based on references from searches of PubMed between 2000 and June 2021. Results: The evidence obtained underscored the tight association between frailty and unfavorable clinical outcomes in older adults with cancer. Given the intrinsic correlation of neuropsychiatric disorders with frailty in the realm of cancer survivorship, the evidence showed they might have a correlation with unfavorable clinical outcomes, late-life geriatric syndromes and higher degree of frailty. Conclusions: The identification of common vulnerabilities among neuropsychiatric disorders, frailty, and cancer may hold promise to unmask similar shared pathways, potentially intercepting targeted new interventions over the spectrum of cancer with the delivery of better pathways of care for older adults with cancer.
Comprehensive Molecular Landscape of Cetuximab Resistance in Head and Neck Cancer Cell Lines
Authors: Gomes Izabela N. F. , da Silva-Oliveira Renato J. , da Silva Luciane Sussuchi , Martinho Olga , Evangelista Adriane F. , van Helvoort Lengert André , Leal Letícia Ferro , Silva Viviane Aline Oliveira , dos Santos Stéphanie Piancenti , Nascimento Flávia Caroline , Lopes Carvalho André , Reis Rui Manuel
Published: January 2022

Cetuximab is the sole anti-EGFR monoclonal antibody that is FDA approved to treat head and neck squamous cell carcinoma (HNSCC). However, no predictive biomarkers of cetuximab response are known for HNSCC. Herein, we address the molecular mechanisms underlying cetuximab resistance in an in vitro model. We established a cetuximab resistant model (FaDu), using increased cetuximab concentrations for more than eight months. The resistance and parental cells were evaluated for cell viability and functional assays. Protein expression was analyzed by Western blot and human cell surface panel by lyoplate. The mutational profile and copy number alterations (CNA) were analyzed using whole-exome sequencing (WES) and the NanoString platform. FaDu resistant clones exhibited at least two-fold higher IC50 compared to the parental cell line. WES showed relevant mutations in several cancer-related genes, and the comparative mRNA expression analysis showed 36 differentially expressed genes associated with EGFR tyrosine kinase inhibitors resistance, RAS, MAPK, and mTOR signaling. Importantly, we observed that overexpression of KRAS, RhoA, and CD44 was associated with cetuximab resistance. Protein analysis revealed EGFR phosphorylation inhibition and mTOR increase in resistant cells. Moreover, the resistant cell line demonstrated an aggressive phenotype with a significant increase in adhesion, the number of colonies, and migration rates. Overall, we identified several molecular alterations in the cetuximab resistant cell line that may constitute novel biomarkers of cetuximab response such as mTOR and RhoA overexpression. These findings indicate new strategies to overcome anti-EGFR resistance in HNSCC.
Pathologic Proteolytic Processing of N-Cadherin as a Marker of Human Fibrotic Disease
Authors: Ferrell Paul Durham , Oristian Kristianne Michelle , Cockrell Everett , Pizzo Salvatore Vincent
Published: January 2022

Prior research has implicated the involvement of cell adhesion molecule N-cadherin in tissue fibrosis and remodeling. We hypothesize that anomalies in N-cadherin protein processing are involved in pathological fibrosis. Diseased tissues associated with fibrosis of the heart, lung, and liver were probed for the precursor form of N-cadherin, pro-N-cadherin (PNC), by immunohistochemistry and compared to healthy tissues. Myofibroblast cell lines were analyzed for cell surface pro-N-cadherin by flow cytometry and immunofluorescent microscopy. Soluble PNC products were immunoprecipitated from patient plasmas and an enzyme-linked immunoassay was developed for quantification. All fibrotic tissues examined show aberrant PNC localization. Cell surface PNC is expressed in myofibroblast cell lines isolated from cardiomyopathy and idiopathic pulmonary fibrosis but not on myofibroblasts isolated from healthy tissues. PNC is elevated in the plasma of patients with cardiomyopathy (p ≤ 0.0001), idiopathic pulmonary fibrosis (p ≤ 0.05), and nonalcoholic fatty liver disease with cirrhosis (p ≤ 0.05). Finally, we have humanized a murine antibody and demonstrate that it significantly inhibits migration of PNC expressing myofibroblasts. Collectively, the aberrant localization of PNC is observed in all fibrotic tissues examined in our study and our data suggest a role for cell surface PNC in the pathogenesis of fibrosis.
LRRK2 at Striatal Synapses: Cell-Type Specificity and Mechanistic Insights
Authors: Skelton Patrick D. , Tokars Valerie , Parisiadou Loukia
Published: January 2022

Mutations in leucine-rich repeat kinase 2 (LRRK2) cause Parkinson’s disease with a similar clinical presentation and progression to idiopathic Parkinson’s disease, and common variation is linked to disease risk. Recapitulation of the genotype in rodent models causes abnormal dopamine release and increases the susceptibility of dopaminergic neurons to insults, making LRRK2 a valuable model for understanding the pathobiology of Parkinson’s disease. It is also a promising druggable target with targeted therapies currently in development. LRRK2 mRNA and protein expression in the brain is highly variable across regions and cellular identities. A growing body of work has demonstrated that pathogenic LRRK2 mutations disrupt striatal synapses before the onset of overt neurodegeneration. Several substrates and interactors of LRRK2 have been identified to potentially mediate these pre-neurodegenerative changes in a cell-type-specific manner. This review discusses the effects of pathogenic LRRK2 mutations in striatal neurons, including cell-type-specific and pathway-specific alterations. It also highlights several LRRK2 effectors that could mediate the alterations to striatal function, including Rabs and protein kinase A. The lessons learned from improving our understanding of the pathogenic effects of LRRK2 mutations in striatal neurons will be applicable to both dissecting the cell-type specificity of LRRK2 function in the transcriptionally diverse subtypes of dopaminergic neurons and also increasing our understanding of basal ganglia development and biology. Finally, it will inform the development of therapeutics for Parkinson’s disease.
Bioengineered Cystinotic Kidney Tubules Recapitulate a Nephropathic Phenotype
Authors: Sendino Garví Elena , Masereeuw Rosalinde , Janssen Manoe J.
Published: January 2022

Nephropathic cystinosis is a rare and severe disease caused by disruptions in the CTNS gene. Cystinosis is characterized by lysosomal cystine accumulation, vesicle trafficking impairment, oxidative stress, and apoptosis. Additionally, cystinotic patients exhibit weakening and leakage of the proximal tubular segment of the nephrons, leading to renal Fanconi syndrome and kidney failure early in life. Current in vitro cystinotic models cannot recapitulate all clinical features of the disease which limits their translational value. Therefore, the development of novel, complex in vitro models that better mimic the disease and exhibit characteristics not compatible with 2-dimensional cell culture is of crucial importance for novel therapies development. In this study, we developed a 3-dimensional bioengineered model of nephropathic cystinosis by culturing conditionally immortalized proximal tubule epithelial cells (ciPTECs) on hollow fiber membranes (HFM). Cystinotic kidney tubules showed lysosomal cystine accumulation, increased autophagy and vesicle trafficking deterioration, the impairment of several metabolic pathways, and the disruption of the epithelial monolayer tightness as compared to control kidney tubules. In particular, the loss of monolayer organization and leakage could be mimicked with the use of the cystinotic kidney tubules, which has not been possible before, using the standard 2-dimensional cell culture. Overall, bioengineered cystinotic kidney tubules recapitulate better the nephropathic phenotype at a molecular, structural, and functional proximal tubule level compared to 2-dimensional cell cultures.
Adipocyte Utx Deficiency Promotes High-Fat Diet-Induced Metabolic Dysfunction in Mice
Authors: Li Fenfen , Wang Shirong , Cui Xin , Jing Jia , Yu Liqing , Xue Bingzhong , Shi Hang
Published: January 2022

While the main function of white adipose tissue (WAT) is to store surplus of energy as triacylglycerol, that of brown adipose tissue (BAT) is to burn energy as heat. Epigenetic mechanisms participate prominently in both WAT and BAT energy metabolism. We previously reported that the histone demethylase ubiquitously transcribed tetratricopeptide (Utx) is a positive regulator of brown adipocyte thermogenesis. Here, we aimed to investigate whether Utx also regulates WAT metabolism in vivo. We generated a mouse model with Utx deficiency in adipocytes (AUTXKO). AUTXKO animals fed a chow diet had higher body weight, more fat mass and impaired glucose tolerance. AUTXKO mice also exhibited cold intolerance with an impaired brown fat thermogenic program. When challenged with high-fat diet (HFD), AUTXKO mice displayed adipose dysfunction featured by suppressed lipogenic pathways, exacerbated inflammation and fibrosis with less fat storage in adipose tissues and more lipid storage in the liver; as a result, AUTXKO mice showed a disturbance in whole body glucose homeostasis and hepatic steatosis. Our data demonstrate that Utx deficiency in adipocytes limits adipose tissue expansion under HFD challenge and induces metabolic dysfunction via adipose tissue remodeling. We conclude that adipocyte Utx is a key regulator of systemic metabolic homeostasis.
Three-Dimensional Finite Element Analysis of Different Connector Designs for All-Ceramic Implant-Supported Fixed Dental Prostheses
Authors: Alberto Laura H. J. , Kalluri Lohitha , Esquivel-Upshaw Josephine F. , Duan Yuanyuan
Published: January 2022

All-ceramic fixed dental prostheses (FDPs) tend to fracture at the connector regions due to high stress concentration at these areas influenced by their design. This study was performed as an adjunct to an existing clinical study to evaluate the influence of the different radii of curvature of gingival embrasure on the stress distribution of a three-unit all-ceramic implanted supported FDP. Three three-dimensional (3D) models were created by scanning two titanium dental implants, their suitable zirconia abutments, and a patient-retrieved dental prosthesis using a micro-CT scanner. The radius of curvature of the gingival embrasure for the distal connector of the FDP was altered to measure 0.25 mm, 0.50 mm, and 0.75 mm. A finite element analysis (FEA) software (ABAQUS) was used to evaluate the impact of different connector designs on the distribution of stresses. Maximum Principal Stress data was collected from the individual components (veneer, framework, and abutments). The radius of curvature of gingival embrasure had a significant influence on the stress distribution at the assessed components. The tensile peak stresses at all structures were highest in the 0.25 mm model, while the 0.50 mm and 0.75 mm models presented similar values and more uniform stress distribution.
Recording the Fragrance of 15 Types of Medicinal Herbs and Comparing Them by Similarity Using the Electronic Nose FF-2A
Authors: Tsuchitani Emi , Nomura Mayumi , Ota Miyuki , Osada Erika , Akiyama Nobutake , Kanegae Yumi , Iwamoto Takeo , Yamaoka Ryuhei , Manome Yoshinobu
Published: January 2022

Medical herbs have been recognized till now as having different constituents that act on the human body. However, the fragrance of herbs is a complex mixture of odors, which makes it difficult to qualify or quantify the scent objectively on the human sense of smell. In this study, aromas of 15 medicinal herbs were recorded using an electronic nose FF-2A, and their characteristics were compared with aroma samples of wine such as Le Nez du Vin, to determine which wine aromas are similar to each medicinal herb. Thereafter, the aromas of the 15 herbs were standardized to create a reference axis for the aroma of each herb, and the similarity of tea herbs to the reference axis was examined. Additionally, the results were compared with those obtained by gas chromatography-mass spectrometry (GC-MS). In FF-2A, the measured scent is recorded as an absolute value. We succeeded in calculating the similarity of the scents of other herbs with the axes of the scent of each herb by standardizing their scents and creating new axis data. Conversely, although GC-MS is able to identify the components and concentrations of fragrances, an electronic nose can analyze fragrances in a way that is uncommon with GC-MS, such as comparison of similarities between fragrances.
Spectroscopic and Computational Study of the Protonation Equilibria of Amino-Substituted benzo[b]thieno[2,3-b]pyrido[1,2-a]benzimidazoles as Novel pH-Sensing Materials
Authors: Perin Nataša , Babić Darko , Kassal Petar , Čikoš Ana , Hranjec Marijana , Vianello Robert
Published: January 2022

We present the synthesis and analytical, spectroscopic and computational characterization of three amino-substituted benzo[b]thieno[2,3-b]pyrido[1,2-a]benzimidazoles as novel pH probes with a potential application in pH-sensing materials. The designed systems differ in the number and position of the introduced isobutylamine groups on the pentacyclic aromatic core, which affects their photophysical and acid-base properties. The latter were investigated by UV-Vis absorption and fluorescence spectroscopies and interpreted by DFT calculations. An excellent agreement in experimentally measured and computationally determined pKa values and electronic excitations suggests that all systems are unionized at neutral pH, while their transition to monocationic forms occurs at pH values between 3 and 5, accompanied by substantial changes in spectroscopic responses that make them suitable for detecting acidic conditions in solutions. Computations identified imidazole imino nitrogen as the most favorable protonation site, further confirmed by analysis of perturbations in the chemical shifts of 1H and 13C NMR, and showed that the resulting basicity emerges as a compromise between the basicity-reducing effect of a nearby nitrile and a favorable contribution from the attached secondary amines. With this in mind, we designed a system with three amino substituents for which calculations predict pKa = 7.0 that we suggest as an excellent starting point for a potential pH sensor able to capture solution changes during the transition from neutral towards acidic media.
The Orthopedic Effects of Electronic Cigarettes: A Systematic Review and Pediatric Case Series
Authors: Armstrong Maxwell Luke , Smith Nicholas , Tracey Rhiannon , Jackman Heather
Published: January 2022

Electronic cigarette (EC) use is highly prevalent, especially in the adolescent population, where 29% of Canadian adolescents have used an EC in the past thirty days per national surveys. Our pediatric orthopedic referral centre observed a cluster of delayed unions of bone fractures in adolescents using ECs and present the case series here. We then asked whether electronic cigarettes impair bone healing or influence orthopedic outcomes. A PRISMA-compliant systematic review was carried out, which revealed no human clinical studies and a general paucity of evidence around ECs and musculoskeletal health. The existing experimental evidence relevant to orthopedics is summarized. The effect of ECs on the musculoskeletal system is poorly understood and is a target for further research.
Intervention to Reduce Anxiety Pre- and Post-Eye Surgery in Pediatric Patients in South Korea: A Preliminary Quasi-Experimental Study
Authors: Yi Hyeran , Lee Hanna
Published: January 2022

In this study, we aimed to identify the effect of preoperative information on postoperative anxiety among children undergoing one-day eye surgery. We utilized a nonequivalent control group and a pretest–posttest quasi-experimental design. The participants were 15 children in the experimental group and 15 children in the control group. Preoperative information was provided to the experimental group in the waiting room. Anxiety level was measured using the Children’s Emotional Manifestation Scale and pulse rate. For pulse rate, there were no statistically significant differences between the groups. In the behavioral anxiety response, there were statistically significant differences between the experimental and control groups (Z = −4.15, p < 0.001). The results suggest that the provision of preoperative information can be an effective intervention for reducing postoperative anxiety and improving the health of children undergoing surgery.
Knowledge, Attitudes, Psychosocial Perspectives and Applied Epidemiology in the Control of Head Lice (pediculosis capitis) in Croatian Preschool Children: A Qualitative Study on Childcare Professionals and Health Coordinators
Authors: Neuberg Marijana , Banfić Ines , Cikač Tina , Ribić Rosana , Zember Sanja , Meštrović Tomislav
Published: January 2022

This study aimed to concurrently determine the perceived knowledge, attitudes and perspectives of childcare professionals working in kindergartens towards pediculosis capitis, a common ectoparasitic disease also known as head lice, as well as gain insights into procedures and control measures that are implemented in an outbreak setting. We used a qualitative approach with a problem-centered, semi-structured and three-part interview technique conducted in selected kindergartens of Varaždin and Međimurje counties of the Republic of Croatia. Based on a purposive (deliberate) sampling method, the study included both childcare professionals and on-site health coordinators aged between 21 and 56 years of age. Five main themes were put forth as a result of the conducted thematic analysis: prevention and control measures for managing head lice, information and knowledge, social issues, psychological issues and disease perception. Each of these themes also had specific emerging categories based on participants’ responses. Even though all respondents confirmed that the disease is continuously monitored only a few of them mentioned that a scalp examination was an inherent part of disease surveillance within the kindergarten community. Moreover, we found that information on pediculosis capitis is available to both parents and childcare professionals, but with a questionable uptake. Additionally, the majority of the respondents reported that parents tend to hide the infestation in their children due to shame and/or to avoid social stigma, and thus often fail to inform kindergarten teachers and health coordinators about the problem. In conclusion, our findings have implications for further practice and the introduction of tailored public health measures for the most vulnerable populations, most notably kindergarten children.
Remote Neuropsychological Intervention for Developmental Dyslexia with the Tachidino Platform: No Reduction in Effectiveness for Older Nor for More Severely Impaired Children
Authors: Lorusso Maria Luisa , Borasio Francesca , Molteni Massimo
Published: January 2022

Tachidino is a web-platform for remote treatment of reading and writing disorders. A total of 91 children with developmental dyslexia and/or dysorthographia participated in the present study and received Tachidino treatment. The purpose of the study was to compare results obtained after four weeks treatment and a six-month follow-up in older versus younger children and in more versus less severely impaired children (separately subdividing them according to reading speed, reading accuracy, and writing accuracy). The results showed no difference in improvement for reading accuracy and speed in the three age groups, but children below 9 years improved more than older children in writing accuracy. Regarding severity groups, children with more severe initial impairments improved more than children with less severe impairments. Additionally, the results were confirmed after controlling for spurious effects due to use of Z-scores and regression to the mean. The findings are discussed in terms of their theoretical and practical implications.
Maintaining Normothermia in Preterm Babies during Stabilisation with an Intact Umbilical Cord
Authors: Cleator Alexander James , Coombe Emma , Alexopoulou Vasiliki , Levingston Laura , Evans Kathryn , Hurst Jonathan Christopher , Yoxall Charles William
Published: January 2022

Background: We had experienced an increase in admission hypothermia rates during implementation of deferred cord clamping (DCC) in our unit. Our objective was to reduce the number of babies with a gestation below 32 weeks who are hypothermic on admission, whilst practising DCC and providing delivery room cuddles (DRC). Method: A 12 month quality improvement project set, in a large Neonatal Intensive Care Unit, from January 2020 to December 2020. Monthly rates of admission hypothermia (<36.5 °C) for all eligible babies, were tracked prospectively. Each hypothermic baby was reviewed as part of a series of Plan, Do, Study Act (PDSA) cycles, to understand potential reasons and to develop solutions. Implementation of these solutions included the dissemination of the learning through a variety of methods. The main outcome measure was the proportion of babies who were hypothermic (<36.5 °C) on admission compared to the previous 12 months. Results: 130 babies with a gestation below 32 weeks were admitted during the study period. 90 babies (69.2%) had DCC and 79 babies (60%) received DRC. Compared to the preceding 12 months, the rate of hypothermia decreased from 25/109 (22.3%) to 13/130 (10%) (p = 0.017). Only 1 baby (0.8%) was admitted with a temperature below 36 °C and 12 babies (9.2%) were admitted with a temperature between 36 °C and 36.4 °C. Continued monitoring during the 3 months after the end of the project showed that the improvements were sustained with 0 cases of hypothermia in 33 consecutive admissions. Conclusions: It is possible to achieve low rates of admission hypothermia in preterm babies whilst providing DCC and DRC. Using a quality improvement approach with PDSA cycles is an effective method of changing clinical practice to improve outcomes.
Dual Synergistic Effects of MgO-GO Fillers on Degradation Behavior, Biocompatibility and Antibacterial Activities of Chitosan Coated Mg Alloy
Authors: Baghbaderani Mohammad Zolfaghari , Abazari Somayeh , Bakhsheshi-Rad Hamid Reza , Ismail Ahmad Fauzi , Sharif Safian , Najafinezhad Aliakbar , Ramakrishna Seeram , Daroonparvar Mohammadreza , Berto Filippo
Published: January 2022

The aim of this work was to establish and characterize chitosan/graphene oxide- magnesium oxide (CS/GO-MgO) nanocomposite coatings on biodegradable magnesium-zinc-cerium (Mg-Zn-Ce) alloy. In comparison to that of pure CS coatings, all composite coatings encapsulating GO-MgO had better adhesion strength to the Mg-Zn-Ce alloy substrate. The result depicted that the co-encapsulation of GO-MgO into the CS layer leads to diminish of contact angle value and hence escalates the hydrophilic characteristic of coated Mg alloy. The electrochemical test demonstrated that the CS/GO-MgO coatings significantly increased the corrosion resistance because of the synergistic effect of the GO and MgO inside the CS coating. The composite coating escalated cell viability and cell differentiation, according to cytocompatibility tests due to the presence of GO and MgO within the CS. The inclusion of GO-MgO in CS film, on the other hand, accelerates the formation of hydroxyapatite (HA) during 14 days immersion in SBF. Immersion results, including weight loss and hydrogen evolution tests, presented that CS/GO-MgO coating enables a considerably reduced degradation rate of Mg-Zn-Ce alloy when compared to the bare alloy. In terms of antibacterial-inhibition properties, the GO-MgO/CS coatings on Mg substrates showed antibacterial activity against Escherichia coli (E. coli), with a large inhibition area around the specimens, particularly for the coating containing a higher concentration of GO-MgO. Bacterial growth was not inhibited by the bare Mg alloy samples. The CS/GO-MgO composite coating is regarded as a great film to enhance the corrosion resistance, bioactivity, and antibacterial performance of Mg alloy implants.
Microrheology to Understand the Viscosity Behavior of a Sophorolipid Biosurfactant
Authors: Kleinen Jochen , Langwald Jan , Venzmer Joachim , Yalcinkaya Hacer
Published: January 2022

The microstructure of the aqueous solutions of purified acidic Sophorolipid (SL) has previously been studied using highly sophisticated methods such as SANS and Cryo-TEM. We were interested in whether (a) the main findings also apply to commercially available SL (which is a mixture of acidic and lactonic SL) and (b) more readily available methods such as DLS can be used to gain insight into the molecular aggregation of SL. Our work was motivated by the increasing interest in biosurfactants for applications in personal and household care. Moreover, the origin behind the more or less lack of rheological response to changes in pH is of practical relevance, as it is somewhat unusual for a carboxylate-group containing surfactant. By using DLS microrheology, we could elucidate the aggregation structure and dynamics of the surfactant on a microscopic scale. Surprisingly, the different degrees of protonation only impacted the microscopic properties such as exchange kinetics and the plateau values of the storage moduli.
Influence of Cooling Scenarios on the Evolution of Microstructures in Nickel-Based Single Crystal Superalloys
Authors: Feng Zhengxing , Wen Zhixun , Lu Guangxian , Zhao Yanchao
Published: January 2022

The reprecipitation and evolution of γ’ precipitates during various cooling approaches from supersolvus temperature are studied experimentally and via phase field simulation in nickel-based single crystal superalloys. The focus of this paper is to explore the influence of cooling methods on the evolution of the morphology and the distribution of γ’ precipitates. It is demonstrated that small and uniform spherical shape γ’ particles formed with air cooling method. When the average cooling rate decreases, the particle number decreases while the average matrix and precipitate channel widths increase. The shape of γ’ precipitates which changed from spherical to cubic and irregular characteristics due to the elastic interaction and elements diffusion are observed with the decrease of the average cooling rate. The phase field simulation results are in good agreement with the experimental results in this paper. The research is a benefit for the study of the rejuvenation heat treatment in re-service nickel-based superalloys.
Four-Year Field Survey of Black Band Disease and Skeletal Growth Anomalies in Encrusting Montipora spp. Corals around Sesoko Island, Okinawa
Authors: Das Rocktim Ramen , Wada Haruka , Masucci Giovanni Diego , Singh Tanya , Tavakoli-Kolour Parviz , Wada Naohisa , Tang Sen-Lin , Yamashiro Hideyuki , Reimer James Davis
Published: January 2022

The Indo-Pacific zooxanthellate scleractinian coral genus Montipora is the host of many coral diseases. Among these are cyanobacterial Black Band Disease (BBD) and Skeletal Growth Anomalies (GAs), but in general data on both diseases are lacking from many regions of the Indo-Pacific, including from Okinawa, southern Japan. In this study, we collected annual prevalence data of Black Band Disease (BBD) and Skeletal Growth Anomalies (GAs) affecting the encrusting form of genus Montipora within the shallow reefs of the subtropical Sesoko Island (off the central west coast of Okinawajima Island) from summer to autumn for four years (2017 to 2020). In 2020 Montipora percent coverage and colony count were also assessed. Generalized Linear Models (GLM) were used to understand the spatial and temporal variation of both BBD and GAs in the nearshore (NE) and reef edge (RE) sites, which revealed higher probability of BBD occurrence in RE sites. BBD prevalence was significantly higher in 2017 in some sites than all other years with site S12 having significant higher probability during all four surveyed years. In terms of GAs, certain sites in 2020 had higher probability of occurrence than during the other years. While the general trend of GAs increased from 2017 to 2020, it was observed to be non-fatal to colonies. In both diseases, the interaction between sites and years was significant. We also observed certain BBD-infected colonies escaping complete mortality. BBD progression rates were monitored in 2020 at site S4, and progression was related to seawater temperatures and was suppressed during periods of heavy rain and large strong typhoons. Our results suggest that higher BBD progression rates are linked with high sea water temperatures (SST > bleaching threshold SST) and higher light levels (>1400 µmol m−2 s−1), indicating the need for further controlled laboratory experiments. The current research will help form the basis for continued future research into these diseases and their causes in Okinawa and the Indo-Pacific Ocean.
Implementation and Evaluation of a Mobile Retinal Image Acquisition System for Screening Diabetic Retinopathy: Study Protocol
Authors: Rêgo Sílvia , Monteiro-Soares Matilde , Dutra-Medeiros Marco , Soares Filipe , Dias Cláudia Camila , Nunes Francisco
Published: January 2022

Screening diabetic retinopathy, a major cause of blindness, is time-consuming for ophthalmologists and has some constrains in achieving full coverage and attendance. The handheld fundus camera EyeFundusScope was recently developed to expand the scale of screening, drawing on images acquired in primary care and telescreening made by ophthalmologists or a computer-aided diagnosis (CADx) system. This study aims to assess the diagnostic accuracy of the interpretation of images captured using EyeFundusScope and perform its technical evaluation, including image quality, functionality, usability, and acceptance in a real-world clinical setting. Physicians and nurses without training in ophthalmology will use EyeFundusScope to take pictures of the retinas of patients with diabetes and the images will be classified for the presence or absence of diabetic retinopathy and image quality by a panel of ophthalmologists. A subgroup of patients will also be examined with the reference standard tabletop fundus camera. Screening results provided by the CADx system on images taken with EyeFundusScope will be compared against the ophthalmologists’ analysis of images taken with the tabletop fundus camera. Diagnostic accuracy measures with 95% confidence intervals (CIs) will be calculated for positive and negative test results. Proportion of each category of image quality will be presented. Usability and acceptance results will be presented qualitatively.
The Application of Artificial Intelligence-Assisted Colposcopy in a Tertiary Care Hospital within a Cervical Pathology Diagnostic Unit
Authors: Zimmer-Stelmach Aleksandra , Zak Jan , Pawlosek Agata , Rosner-Tenerowicz Anna , Budny-Winska Joanna , Pomorski Michal , Fuchs Tomasz , Zimmer Mariusz
Published: January 2022

The rising global incidence of cervical cancer is estimated to have affected more than 600,000 women, and nearly 350,000 women are predicted to have died from the disease in 2020 alone. Novel advances in cancer prevention, screening, diagnosis and treatment have all but reduced the burden of cervical cancer in developed nations. Unfortunately, cervical cancer is still the number one gynecological cancer globally. A limiting factor in managing cervical cancer globally is access to healthcare systems and trained medical personnel. Any methodology or procedure that may simplify or assist cervical cancer screening is desirable. Herein, we assess the use of artificial intelligence (AI)-assisted colposcopy in a tertiary hospital cervical diagnostic pathology unit. The study group consisted of 48 women (mean age 34) who were referred to the clinic for a routine colposcopy by their gynecologist. Cervical images were taken by an EVA-Visualcheck TM colposcope and run through an AI algorithm that gave real-time binary results of the cervical images as being either normal or abnormal. The primary endpoint of the study assessed the AI algorithm’s ability to correctly identify histopathology results of CIN2+ as being abnormal. A secondary endpoint was a comparison between the AI algorithm and the clinical assessment results. Overall, we saw lower sensitivity of AI (66.7%; 12/18) compared with the clinical assessment (100%; 18/18), and histopathology results as the gold standard. The positive predictive value (PPV) was comparable between AI (42.9%; 12/28) and the clinical assessment (41.8%; 18/43). The specificity, however, was higher in the AI algorithm (46.7%; 14/30) compared to the clinical assessment (16.7%; 5/30). Comparing the congruence between the AI algorithm and histopathology results showed agreement 54.2% of the time and disagreement 45.8% of the time. A trained colposcopist was in agreement 47.9% and disagreement 52.1% of the time. Assessing these results, there is currently no added benefit of using the AI algorithm as a tool of speeding up diagnosis. However, given the steady improvements in the AI field, we believe that AI-assisted colposcopy may be of use in the future.
Synchronous Generative Development amidst Situated Entropy
Authors: Fox Stephen
Published: January 2022

The Sustainable Development Goals have been criticized for not providing sufficient balance between human well-being and environmental well-being. By contrast, joint agent-environment systems theory is focused on reciprocal synchronous generative development. The purpose of this paper is to extend this theory towards practical application in sustainable development projects. This purpose is fulfilled through three interrelated contributions. First, a practitioner description of the theory is provided. Then, the theory is extended through reference to research concerned with multilevel pragmatics, competing signals, commitment processes, technological mediation, and psychomotor functioning. In addition, the theory is related to human-driven biosocial-technical innovation through the example of digital twins for agroecological urban farming. Digital twins being digital models that mirror physical processes; that are connected to physical processes through, for example, sensors and actuators; and which carry out analyses of physical processes in order to improve their performance. Together, these contributions extend extant theory towards application for synchronous generative development that balances human well-being and environmental well-being. However, the practical examples in the paper indicate that counterproductive complexity can arise from situated entropy amidst biosocial-technical innovations: even when those innovations are compatible with synchronous generative development.
Dynamic Linkages among Saudi Market Sectors Indices
Authors: Altahtamouni Farouq , Masfer Hajar , Alyousef Shikhah
Published: January 2022

This study aims to test the causal relationship between Saudi stock market index (TASI) and sectoral indices throughout the period from 2016–2020. The study data were extracted through the main index of the Saudi market and the indices of the available data of 19 sectors out of 21 sectors. The unit root test was used along with the Granger causality test, in addition to multiple regression tests in order to analyze the study hypotheses. The study shows that all index series were stationary at the zero level I (0), and the results also show that there were bidirectional and unidirectional causal relationships between TASI and sectoral indices, and that TASI effectively mirrors all the changes that occur in the Saudi stock market.
Usefulness of Digital Serious Games in Engineering for Diverse Undergraduate Students
Authors: Cook-Chennault Kimberly , Villanueva Alarcón Idalis , Jacob Gabrielle
Published: January 2022

The use of educational digital games as supplemental tools to course instruction materials has increased over the last several decades and especially since the COVID-19 pandemic. Though these types of instructional games have been employed in the majority of STEM disciplines, less is known about how diverse populations of students interpret and define the value of these games towards achieving academic and professional pursuits. A mixed-method sequential exploratory research design method that was framed on the Technology Acceptance Model, Game-Based Learning Theory and Expectancy Value Theory was used to examine how 201 students perceived the usefulness of an intuitive education game that was designed to teach engineering mechanics used in designing civil structures. We found that students had different expectations of educational digital games than games designed for entertainment used outside of classroom environments. Several students thought that the ability to design their own structures and observe structure failure in real-time was a valuable asset in understanding how truss structures responded to physical loading conditions. However, few students thought the educational game would be useful for exam (14/26) or job interview (19/26) preparation. Students associated more value with engineering games that illustrate course content and mathematical calculations used in STEM courses than those that do not include these elements.
Critical Thinking and Effective Personality in the Framework of Education for Sustainable Development
Authors: Merma-Molina Gladys , Gavilán-Martín Diego , Baena-Morales Salvador , Urrea-Solano Mayra
Published: January 2022

According to UNESCO, critical thinking (CT) is a strategic skill for the 21st century, as it is associated with attitudes of personal and social change and improvement. Based on this, the objectives of this study were (1) to find out the possible relationships between CT and effective personality (EP) and (2) to determine the extent to which EP predicts the development of CT in children and adolescents. The research approach was quantitative, correlational and predictive. The sample consisted of 562 Spanish students. The measurement instruments were: (1) the Critical Thinking Disposition Inventory and (2) the Efficacy Personality Questionnaire. The main findings showed that there is a positive relationship between EP and CT, with the traits “being enthusiastic”, “developing positive self-esteem” and “having self-confidence” correlating most strongly with CT. The regression analysis shows that EP influences the development of students’ CT. There were no significant differences according to sex, and Primary Education students obtained higher CT scores. It is concluded that the better the promotion of EP, the greater the CT development will be. This suggests the need to design educational programmes for the improvement of EP, especially in adolescents.