bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluating the Genetic Effects of Gut Microbiota on the Development of Neuroticism and General Happiness: A Polygenic Score Analysis and Interaction Study Using UK Biobank Data
Authors: Jia Yumeng , Cheng Shiqiang , Liu Li , Cheng Bolun , Liang Chujun , Ye Jing , Chu Xiaomeng , Yao Yao , Wen Yan , Kafle Om Prakash , Zhang Feng
Published: January 2023

Limited efforts have been invested in exploring the interaction effects between genetic factors and gut microbiota on neuroticism and general happiness. The polygenic risk scores (PRS) of gut microbiota were calculated from individual-level genotype data of the UK Biobank cohort. Linear regression models were then used to assess the associations between individual PRS of gut microbiota and mental traits and interaction analysis was performed by PLINK2.0. KOBAS-i was used to conduct gene ontology (GO) enrichment analysis of the identified genes. We observed suggestive significant associations between neuroticism and PRS for the genus Bifidobacterium (rank-normal transformation, RNT) (beta = −1.10, P = 4.16 × 10−3) and the genus Desulfovibrio (RNT) (beta = 0.54, P = 7.46 × 10−3). PRS for the genus Bifidobacterium (hurdle binary, HB) (beta = 1.99, P = 5.24 × 10−3) and the genus Clostridium (RNT) (beta = 1.26, P = 9.27 × 10−3) were found to be suggestive positively associated with general happiness. Interaction analysis identified several significant genes that interacted with gut microbiota, such as RORA (rs575949009, beta = −45.00, P = 1.82 × 10−9) for neuroticism and ASTN2 (rs36005728, beta = 19.15, P = 3.37 × 10−8) for general happiness. Our study results support the genetic effects of gut microbiota on the development of neuroticism and general happiness.
Psychrophilic Yeasts: Insights into Their Adaptability to Extremely Cold Environments
Authors: Liu Haisheng , Zheng Guiliang , Chen Zhongwei , Ding Xiaoya , Wu Jinran , Zhang Haili , Jia Shulei
Published: January 2023

Psychrophilic yeasts are distributed widely on Earth and have developed adaptation strategies to overcome the effect of low temperatures. They can adapt to low temperatures better than bacteriophyta. However, to date, their whole-genome sequences have been limited to the analysis of single strains of psychrophilic yeasts, which cannot be used to reveal their possible psychrophilic mechanisms to adapt to low temperatures accurately and comprehensively. This study aimed to compare different sources of psychrophilic yeasts at the genomic level and investigate their cold-adaptability mechanisms in a comprehensive manner. Nine genomes of known psychrophilic yeasts and three representative genomes of mesophilic yeasts were collected and annotated. Comparative genomic analysis was performed to compare the differences in their signaling pathways, metabolic regulations, evolution, and psychrophilic genes. The results showed that fatty acid desaturase coding genes are universal and diverse in psychophilic yeasts, and different numbers of these genes exist (delta 6, delta 9, delta 12, and delta 15) in the genomes of various psychrophilic yeasts. Therefore, they can synthesize polyunsaturated fatty acids (PUFAs) in a variety of ways and may be able to enhance the fluidity of cell membranes at low temperatures by synthesizing C18:3 or C18:4 PUFAs, thereby ensuring their ability to adapt to low-temperature environments. However, mesophilic yeasts have lost most of these genes. In this study, psychrophilic yeasts could adapt to low temperatures primarily by synthesizing PUFAs and diverse antifreeze proteins. A comparison of more psychrophilic yeasts’ genomes will be useful for the study of their psychrophilic mechanisms, given the presence of additional potential psychrophilic-related genes in the genomes of psychrophilic yeasts. This study provides a reference for the study of the psychrophilic mechanisms of psychrophilic yeasts.
Prenatal Screening and Diagnostic Considerations for 22q11.2 Microdeletions
Authors: Blagowidow Natalie , Nowakowska Beata , Schindewolf Erica , Grati Francesca Romana , Putotto Carolina , Breckpot Jeroen , Swillen Ann , Crowley Terrence Blaine , Loo Joanne C. Y. , Lairson Lauren A. , Óskarsdóttir Sólveig , Boot Erik , Garcia-Minaur Sixto , Cristina Digilio Maria , Marino Bruno , Coleman Beverly , Moldenhauer Julie S. , Bassett Anne S. , McDonald-McGinn Donna M.
Published: January 2023

Diagnosis of a chromosome 22q11.2 microdeletion and its associated deletion syndrome (22q11.2DS) is optimally made early. We reviewed the available literature to provide contemporary guidance and recommendations related to the prenatal period. Indications for prenatal diagnostic testing include a parent or child with the 22q11.2 microdeletion or suggestive prenatal screening results. Definitive diagnosis by genetic testing of chorionic villi or amniocytes using a chromosomal microarray will detect clinically relevant microdeletions. Screening options include noninvasive prenatal screening (NIPS) and imaging. The potential benefits and limitations of each screening method should be clearly conveyed. NIPS, a genetic option available from 10 weeks gestational age, has a 70–83% detection rate and a 40–50% PPV for most associated 22q11.2 microdeletions. Prenatal imaging, usually by ultrasound, can detect several physical features associated with 22q11.2DS. Findings vary, related to detection methods, gestational age, and relative specificity. Conotruncal cardiac anomalies are more strongly associated than skeletal, urinary tract, or other congenital anomalies such as thymic hypoplasia or cavum septi pellucidi dilatation. Among others, intrauterine growth restriction and polyhydramnios are additional associated, prenatally detectable signs. Preconception genetic counselling should be offered to males and females with 22q11.2DS, as there is a 50% risk of transmission in each pregnancy. A previous history of a de novo 22q11.2 microdeletion conveys a low risk of recurrence. Prenatal genetic counselling includes an offer of screening or diagnostic testing and discussion of results. The goal is to facilitate optimal perinatal care.
What Have We Learned from Patients Who Have Arboleda-Tham Syndrome Due to a De Novo KAT6A Pathogenic Variant with Impaired Histone Acetyltransferase Function? A Precise Clinical Description May Be Critical for Genetic Testing Approach and Final Diagnosis
Authors: Bukvic Nenad , Chetta Massimiliano , Bagnulo Rosanna , Leotta Valentina , Pantaleo Antonino , Palumbo Orazio , Palumbo Pietro , Oro Maria , Rivieccio Maria , Laforgia Nicola , De Rinaldis Marta , Rosati Alessandra , Kerkhof Jennifer , Sadikovic Bekim , Resta Nicoletta
Published: January 2023

Pathogenic variants in genes are involved in histone acetylation and deacetylation resulting in congenital anomalies, with most patients displaying a neurodevelopmental disorder and dysmorphism. Arboleda-Tham syndrome caused by pathogenic variants in KAT6A (Lysine Acetyltransferase 6A; OMIM 601408) has been recently described as a new neurodevelopmental disorder. Herein, we describe a patient characterized by complex phenotype subsequently diagnosed using the clinical exome sequencing (CES) with Arboleda-Tham syndrome (ARTHS; OMIM 616268). The analysis revealed the presence of de novo pathogenic variant in KAT6A gene, a nucleotide c.3385C>T substitution that introduces a premature termination codon (p.Arg1129*). The need for straight multidisciplinary collaboration and accurate clinical description findings (bowel obstruction/megacolon/intestinal malrotation) was emphasized, together with the utility of CES in establishing an etiological basis in clinical and genetical heterogeneous conditions. Therefore, considering the phenotypic characteristics, the condition’s rarity and the reviewed literature, we propose additional diagnostic criteria that could help in the development of future clinical diagnostic guidelines. This was possible thanks to objective examinations performed during the long follow-up period, which permitted scrupulous registration of phenotypic changes over time to further assess this rare disorder. Finally, given that different genetic syndromes are associated with distinct genomic DNA methylation patterns used for diagnostic testing and/or as biomarker of disease, a specific episignature for ARTHS has been identified.
Comparison between the Gametophyte and the Sporophyte Transcriptomes of the Endangered Fern Vandenboschia speciosa
Authors: Martín-Blázquez Rubén , Bakkali Mohammed , Ruiz-Estévez Mercedes , Garrido-Ramos Manuel A.
Published: January 2023

Genomic resources are essential to understanding the evolution and functional biology of organisms. Nevertheless, generating genomic resources from endangered species may be challenging due to the scarcity of available specimens and sampling difficulties. In this study, we compare the transcriptomes of the sporophyte and the gametophyte of the endangered fern Vandenboschia speciosa. After Illumina sequencing and de novo transcriptome assembly of the gametophyte, annotation proved the existence of cross-species contamination in the gametophyte sample. Thus, we developed an in silico decontamination step for the gametophyte sequences. Once the quality check of the decontaminated reads passed, we produced a de novo assembly with the decontaminated gametophyte reads (with 43,139 contigs) and another combining the sporophyte and in silico decontaminated gametophyte reads (with 42,918 contigs). A comparison of the enriched GO terms from the top 1000 most expressed transcripts from both tissues showed that the gametophyte GO term set was enriched in sequences involved in development, response to stress, and plastid organization, while the sporophyte GO term set had a larger representation of more general metabolic functions. This study complements the available genomic resources on the life cycle of the endangered fern Vandenboschia speciosa.
Nontraditional Roles of DNA Polymerase Eta Support Genome Duplication and Stability
Authors: Eckert Kristin A.
Published: January 2023

DNA polymerase eta (Pol η) is a Y-family polymerase and the product of the POLH gene. Autosomal recessive inheritance of POLH mutations is the cause of the xeroderma pigmentosum variant, a cancer predisposition syndrome. This review summarizes mounting evidence for expanded Pol η cellular functions in addition to DNA lesion bypass that are critical for maintaining genome stability. In vitro, Pol η displays efficient DNA synthesis through difficult-to-replicate sequences, catalyzes D-loop extensions, and utilizes RNA–DNA hybrid templates. Human Pol η is constitutively present at the replication fork. In response to replication stress, Pol η is upregulated at the transcriptional and protein levels, and post-translational modifications regulate its localization to chromatin. Numerous studies show that Pol η is required for efficient common fragile site replication and stability. Additionally, Pol η can be recruited to stalled replication forks through protein–protein interactions, suggesting a broader role in replication fork recovery. During somatic hypermutations, Pol η is recruited by mismatch repair proteins and is essential for VH gene A:T basepair mutagenesis. Within the global context of repeat-dense genomes, the recruitment of Pol η to perform specialized functions during replication could promote genome stability by interrupting pure repeat arrays with base substitutions. Alternatively, not engaging Pol η in genome duplication is costly, as the absence of Pol η leads to incomplete replication and increased chromosomal instability.
Analysis of Complete Chloroplast Genome: Structure, Phylogenetic Relationships of Galega orientalis and Evolutionary Inference of Galegeae
Authors: Feng Junjie , Xiong Yi , Su Xiaoli , Liu Tianqi , Xiong Yanli , Zhao Junming , Lei Xiong , Yan Lijun , Gou Wenlong , Ma Xiao
Published: January 2023

Galega orientalis, a leguminous herb in the Fabaceae family, is an ecologically and economically important species widely cultivated for its strong stress resistance and high protein content. However, genomic information of Galega orientalis has not been reported, which limiting its evolutionary analysis. The small genome size makes chloroplast relatively easy to obtain genomic sequence for phylogenetic studies and molecular marker development. Here, the chloroplast genome of Galega orientalis was sequenced and annotated. The results showed that the chloroplast genome of G. orientalis is 125,280 bp in length with GC content of 34.11%. A total of 107 genes were identified, including 74 protein-coding genes, 29 tRNAs and four rRNAs. One inverted repeat (IR) region was lost in the chloroplast genome of G. orientalis. In addition, five genes (rpl22, ycf2, rps16, trnE-UUC and pbf1) were lost compared with the chloroplast genome of its related species G. officinalis. A total of 84 long repeats and 68 simple sequence repeats were detected, which could be used as potential markers in the genetic studies of G. orientalis and related species. We found that the Ka/Ks values of three genes petL, rpl20, and ycf4 were higher than one in the pairwise comparation of G. officinalis and other three Galegeae species (Calophaca sinica, Caragana jubata, Caragana korshinskii), which indicated those three genes were under positive selection. A comparative genomic analysis of 15 Galegeae species showed that most conserved non-coding sequence regions and two genic regions (ycf1 and clpP) were highly divergent, which could be used as DNA barcodes for rapid and accurate species identification. Phylogenetic trees constructed based on the ycf1 and clpP genes confirmed the evolutionary relationships among Galegeae species. In addition, among the 15 Galegeae species analyzed, Galega orientalis had a unique 30-bp intron in the ycf1 gene and Tibetia liangshanensis lacked two introns in the clpP gene, which is contrary to existing conclusion that only Glycyrrhiza species in the IR lacking clade (IRLC) lack two introns. In conclusion, for the first time, the complete chloroplast genome of G. orientalis was determined and annotated, which could provide insights into the unsolved evolutionary relationships within the genus Galegeae.
Impact of COVID-19 on Patients Hospitalized with ST-Segment Elevation Myocardial Infarction in the United States during the Early Pandemic: An Analysis of Outcomes, Care Delivery, and Racial Disparities in Mortality
Authors: Majeed Harris , Gangu Karthik , Shekhar Rahul , Sagheer Shazib , Garg Ishan , Shuja Hina , Bobba Aniesh , Chourasia Prabal , Avula Sindhu Reddy , Sheikh Abu Baker
Published: January 2023

The COVID-19 pandemic has impacted healthcare delivery to patients with ST-segment elevation myocardial infarction (STEMI). The aim of our retrospective study was to determine the effect of COVID-19 on inpatient STEMI outcomes and to investigate changes in cardiac care delivery during 2020. We utilized the National Inpatient Sample database to examine inpatient mortality and cardiac procedures among STEMI patients with and without COVID-19. In our study, STEMI patients with COVID-19 had higher inpatient mortality (47.4% vs. 11.2%, aOR: 3.8, 95% CI: 3.2–4.6, p < 0.001), increased length of stay (9.0 days vs. 4.3 days, p < 0.001) and higher cost of hospitalization (USD 172,518 vs. USD 131,841, p = 0.004) when compared to STEMI patients without COVID-19. STEMI patients with COVID-19 also received significantly less invasive cardiac procedures (coronary angiograms: 30.4% vs. 50.8%, p < 0.001; PCI: 32.9% vs. 70.1%, p < 0.001; CABG: 0.9% vs. 4.1%, p < 0.001) and were more likely to receive systemic thrombolytic therapy (4.2% vs. 1.1%, p < 0.001) when compared to STEMI patients without COVID-19. Our findings are the result of complications of SARS-CoV2 infection as well as alterations in healthcare delivery due to the burden of the COVID-19 pandemic.
A Habitable Earth and Carbon Neutrality: Mission and Challenges Facing Resources and the Environment in China—An Overview
Authors: Zhang Min , Qiu Yan , Li Chunling , Cui Tao , Yang Mingxing , Yan Jun , Yang Wu
Published: January 2023

Since the Industrial Revolution, the impacts of human activities have changed the global climate system, and climate warming has had rapid and widespread effects on the planet. At present, the world is experiencing a series of natural disasters, such as climate change, environmental pollution, biodiversity loss, and sea level rise, which pose a serious threat to the livability of the Earth. An international consensus has been reached that achieving carbon neutrality is the key to tackling climate change; it is also crucial to building a livable planet. To achieve carbon neutrality, energy is the main aspect, for which technology regarding resources and the environment is essential. In this context, we collected data, performed an in-depth analysis of the basic and structural characteristics of the development of the coal industry and environmental remediation, studied and judged the trends in regional economic development and demand growth, and closely examined the requirements of China’s development strategy, which focuses on the ideas of carbon peak and carbon neutralization in line with local development trends and economic system characteristics. We must build a livable Earth, promote the green and low-carbon transformation of regional energy, promote high-quality economic development, and ensure the safe supply of energy.
Comparison of Visual Skills between Federated and Non-Federated Athletes
Authors: Sánchez-Tena Miguel Ángel , Rodríguez-Alonso Xabier , Martinez-Perez Clara , Tornero-Aguilera José Francisco , Clemente-Suárez Vicente J. , Sanchez-Ramos Celia , Alvarez-Peregrina Cristina
Published: January 2023

Background: To perform motor tasks, athletes must gather a considerable amount of visual information quickly. Evidence shows that visual skills vary between athletes and non-athletes, and impact athletic performance. However, there is no scientific evidence suggesting that there are any differences between the visual skills of federated and non-federated athletes. As such, the objective of this paper was to compare how visual skills influence the sports performance of federated and non-federated athletes, respectively. Methods: A visual examination has been conducted on a total of 52 athletes between 18 and 37 years of age. The COI-Sport Vision system screen (International Optometry Center, Madrid, Spain) was used to examine static visual acuity, dynamic visual acuity, contrast sensitivity, stereopsis, fixation disparity, visual memory, identification, anticipation time, peripheral awareness, and hand-eye coordination. Results: On average, federated athletes train more hours per day than non-federated athletes (1.4 ± 0.8) (p = 0.046). A significant correlation was observed between the average time of visual memory (β = −0.0683, p < 0.001), the average time of anticipation (β = 0.006, p = 0.009), the average time of peripheral awareness (β = 0.026, p = 0.002), hand-eye coordination (β = 0.028, p = 0.004), dynamic visual acuity (β = 0.055, p < 0.001), and the number of training hours. Conclusion: Results suggest that federated athletes are more concerned about their ocular health. Nonetheless, no differences were found in the oculomotor skills of both groups. Further investigation is required to consider each sport discipline individually.
Concentration Characteristics and Correlations with Other Pollutants of Atmospheric Particulate Matter as Affected by Relevant Policies
Authors: Song Hong , Dong Yuhang , Yang Jiayu , Zhang Xin , Nie Xingxin , Fan Yuesheng
Published: January 2023

With the increase in global environmental pollution, it is important to understand the concentration characteristics and correlations with other pollutants of atmospheric particulate matter as affected by relevant policies. The data presented in this paper were obtained at monitoring stations in Xi’an, China, in the years from 2016 to 2020, and the spatial distribution characteristics of the mass and quantity concentrations of particulate matter in the atmosphere, as well as its correlation with other pollutants, were analyzed in depth. The results showed that the annual average concentrations of PM10 and PM2.5 decreased year by year from 2016 to 2020. The annual concentrations of PM2.5 decreased by 20.3 μg/m3, and the annual concentrations of PM10 decreased by 47.3 μg/m3. The days with concentrations of PM10 exceeding the standards decreased by 82 days, with a decrease of 66.7%. The days with concentrations of PM2.5 exceeding the standards decreased by 40 days, with a decrease of 35.4%. The concentration values of PM10 and PM2.5 were roughly consistent with the monthly and daily trends. The change in monthly concentrations was U-shaped, and the change in daily concentrations showed a double-peak behavior. The highest concentrations of particulate matter appeared at about 8:00~9:00 am and 11:00 pm, and they were greatly affected by human activity. The proportion of particles of 0~1.0 μm decreased by 1.94%, and the proportion of particles of 0~2.5 μm decreased by 2.00% from 2016 to 2020. A multivariate linear regression model to calculate the concentrations of the pollutants was established. This study provides a reference for the comprehensive analysis and control of air pollutants in Xi’an and even worldwide.
Occurrence of Hepatitis A Virus in Water Matrices: A Systematic Review and Meta-Analysis
Authors: Takuissu Guy Roussel , Kenmoe Sebastien , Ebogo-Belobo Jean Thierry , Kengne-Ndé Cyprien , Mbaga Donatien Serge , Bowo-Ngandji Arnol , Ndzie Ondigui Juliette Laure , Kenfack-Momo Raoul , Tchatchouang Serges , Kenfack-Zanguim Josiane , Lontuo Fogang Robertine , Zeuko’o Menkem Elisabeth , Kame-Ngasse Ginette Irma , Magoudjou-Pekam Jeannette Nina , Veneri Carolina , Mancini Pamela , Bonanno Ferraro Giusy , Iaconelli Marcello , Orlandi Lidia , Del Giudice Claudia , Suffredini Elisabetta , La Rosa Giuseppina
Published: January 2023

Hepatitis A is a common form of viral hepatitis. It is usually transmitted through the ingestion of contaminated food and water. This systematic review was carried out to summarise the overall prevalence of Hepatitis A virus (HAV) in different water matrices: untreated and treated wastewater, surface water, groundwater, drinking water, and others (e.g., irrigation water and floodwater). The literature search was performed in four databases: PubMed, Web of Science, Global Index Medicus, and Excerpta Medica Database. Heterogeneity (I2) was assessed using the χ2 test on the Cochran Q statistic and H parameters. A total of 200 prevalence data from 144 articles were included in this meta-analysis. The overall prevalence of HAV in water matrices was 16.7% (95% CI: 13.4–20.3). The prevalence for individual matrix was as follows: 31.4% (95% CI: 23.0–40.4) untreated wastewater, 18.0% (95% CI: 9.5–28.2) treated wastewater, 15.0% (95% CI: 10.1–20.5) surface water, 2.3% (95% CI: 0.1–6.0) in groundwater, 0.3% (95% CI: 0.0–1.7) in drinking water, and 8.5% (95% CI: 3.1–15.6) in other matrices. The prevalence was higher in low-income economies (29.0%). Africa and Eastern Mediterranean were the regions with higher HAV prevalence values. This study showed a high heterogeneity (I2 > 75%) with a significant publication bias (p value Egger test < 0.001). The results of this review suggest that water matrices could be an important route of HAV transmission even in industrialized countries, despite the lower prevalence compared to less industrialized countries, and the availability of advanced water management systems. More effective water/wastewater treatment strategies are needed in developing countries to limit the environmental circulation of HAV.
Sustainable Return to Work for Workers with Mental Health and Musculoskeletal Conditions
Authors: Etuknwa Abasiama , Daniels Kevin , Nayani Rachel , Eib Constanze
Published: January 2023

Common mental health and musculoskeletal disorders (CMDs and MSDs) are two of the most significant causes of non-participation in employment amongst working age adults. Background: This case study fills an important gap in the scientific literature on reintegration back to work after sickness absence due to CMDs and MSDs. It particularly examines the return to work (RTW) experiences of sick-listed employees to understand the facilitators and barriers of sustainable RTW. Methods: Using a realist evaluation approach within a qualitative inquiry, perceptions of employees were explored to provide in-depth understanding of what, how and under what circumstances sustainable RTW can be enabled for employees absent on a short- or long-term basis. Repeat face-to-face semi-structured interviews were conducted with 22 participants (15 women and 7 men, aged 30–50 years and sick-listed with MSDs and CMDs) who were recruited using purposive sampling. Data was thematically analysed. Results: A total of 2 main codes and 5 subcodes were developed and grouped into three theoretical abstractions. As a result of validating the context, mechanism, and outcome configurations with accounts of participants, all three initial theories explaining the most prominent mechanisms that either facilitates or impedes a sustainable RTW for people with CMDs and MSDs were justified. Conclusions: Our findings reveal the active role of line managers on the RTW outcomes of returning employees. However, line-manager’s competence and ability to effectively support and implement appropriate RTW strategies suited to employees’ hinges on working in alignment with key stakeholders and returning employees.
Hand Hygiene, Face Mask Use, and Associated Factors during the COVID-19 Pandemic among the Students of Mongar Higher Secondary School, Bhutan: A Cross-Sectional Study
Authors: Wangchuk Tashi , Kinga  , Wangdi Ugyen , Tshering Ugyen , Wangdi Kinley
Published: January 2023

Non-pharmacological measures, such as hand hygiene and face mask use, continue to play an important role in the fight against the COVID-19 pandemic. However, there is a paucity of studies on the adherence to these measures among students in Bhutan. Therefore, we aimed to investigate hand hygiene and face mask-wearing behaviours, as well as their associated factors, among the students of Mongar Higher Secondary School, Bhutan. We conducted a cross-sectional study amongst the students of Mongar Higher Secondary School in Bhutan. The students self-answered the questionnaire on web-based Google Forms. Multivariable logistic regression for good hand washing and face mask use was conducted in order to identify statistically significant socio-demographic covariates. The correlation between hand hygiene and mask use was investigated using Pearson’s correlation coefficient. A total of 533 students completed the survey questionnaire, 52.9% (282) of whom were female students. Facebook (44.3%, 236) and TV (35.5%, 189) were the two most popular sources of information on COVID-19 prevention and control. Good (scores of ≥80% of total scores) hand hygiene and face mask use were reported in 33.6% (179) and 22.1% (118) of students. In multivariable logistic regression, male students presented 79% (adjusted odds ratio [AOR] = 1.79, 95% confidence interval [CI] = 1.23–2.613) odds of engaging in good hand hygiene, compared to female students. Compared to grade 9, those in grade 10 were 60% (AOR = 0.4, 95% CI 0.158–0.998) less likely to engage in good hand hygiene. Boarding students presented 68% (AOR = 1.68, 95% CI 1.001, 2.813) higher odds of wearing a face mask compared to day students. There was a significant positive correlation between good hand hygiene and face mask use (r = 0.3671, p-value < 0.001). Good hand hygiene and face mask use were reported in less than one-third of the study participants. It is recommended to continue educating students on good hand hygiene and face mask use through popular information sources.
Mn Pretreatment Improves the Physiological Resistance and Root Exudation of Celosia argentea Linn. to Cadmium Stress
Authors: You Shaohong , Deng Zhenliang , Chen Mouyixing , Zheng Yingyi , Liu Jiu , Jiang Pingping
Published: January 2023

Phytoextraction using Celosia argentea Linn. by Mn pretreatment can potentially decontaminate Cd-contaminated soils. However, the mechanism that accelerates the Cd bioaccumulation is still unknown. In order to study the effect and mechanism of Mn pretreatment on Cd bioaccumulation in C. argentea, the hydroponic experiments were set to determine the chlorophyll content, antioxidant enzyme activity, malondialdehyde content, and root exudation of C. argentea. The results indicated that after seven days of Mn pretreatment, both the biomass and Cd concentrations in plants increased compared to the control group. One of the mechanisms for this was the improvement in the physiological resistance of C. argentea following pretreatment with Mn. Compared with Cd stress alone, Mn pretreatment increased photosynthesis and reduced membrane lipid peroxidation. Meanwhile, the activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX) were significantly reduced in leaves of C. argentea after Mn pretreatment through the reduction in the production of reactive oxygen species. In addition, Mn promoted the exudation of organic acids in the roots of C. argentea. The contents of citric and malic acids increased by 55.3% and 26.4%, respectively, which may be another important reason for Mn pretreatment increasing Cd bioaccumulation in C. argentea. Therefore, the present work shows that the pretreatment of seedlings with Mn can provide a meaningful strategy to improve the remediation efficiency of Cd-contaminated soils by C. argentea.
Online Health Information Seeking: An Italian Case Study for Analyzing Citizens’ Behavior and Perception
Authors: D’Andrea Alessia , Grifoni Patrizia , Ferri Fernando
Published: January 2023

This study aims to understand people’s behavior when searching for online health information (and COVID-19 information) and their perception of the trustworthiness and credibility of the searched information, the actors, and sources used to obtain it. A questionnaire addressed to people who permanently live in Italy between ages 19 and 60 has been used to collect data. Data extracted from the analysis are reassuring from the point of view of trust and credibility both in the actors and in the sources used to obtain information on health and COVID-19. A correlation between the analyzed individual features, the online health information-seeking behavior, and perception resulted from the analysis. The study also underlined a positive correlation between the perception of the influence of information on the knowledge of health problems and the ability to identify false online health information, and between the experience in detecting false health online information and the ability to detect it. Finally, a positive correlation also resulted between the experience in finding online health information and the experience in finding false COVID-19 information.
Factors Influencing the Adoption of Magnetic Resonance-Guided High-Intensity Focused Ultrasound for Painful Bone Metastases in Europe, A Group Concept Mapping Study
Authors: Simões Corrêa Galendi Julia , Siefen Ann-Cathrine , Moretti Debora M. , Yeo Sin Yuin , Grüll Holger , Bratke Grischa , Morganti Alessio Giuseppe , Bazzocchi Alberto , Gasperini Chiara , De Felice Francesca , Blanco Sequeiros Roberto , Huhtala Mira , Nijholt Ingrid M. , Boomsma Martijn F. , Bos Clemens , Verkooijen Helena M. , Müller Dirk , Stock Stephanie
Published: January 2023

Magnetic resonance imaging-guided high-intensity focused ultrasound (MR-HIFU) is an innovative treatment for patients with painful bone metastases. The adoption of MR-HIFU will be influenced by several factors beyond its effectiveness. To identify contextual factors affecting the adoption of MR-HIFU, we conducted a group concept mapping (GCM) study in four European countries. The GCM was conducted in two phases. First, the participants brainstormed statements guided by the focus prompt “One factor that may influence the uptake of MR-HIFU in clinical practice is...”. Second, the participants sorted statements into categories and rated the statements according to their importance and changeability. To generate a concept map, multidimensional scaling and cluster analysis were conducted, and average ratings for each (cluster of) factors were calculated. Forty-five participants contributed to phase I and/or II (56% overall participation rate). The resulting concept map comprises 49 factors, organized in 12 clusters: “competitive treatments”, “physicians’ attitudes”, “alignment of resources”, “logistics and workflow”, “technical disadvantages”, “radiotherapy as first-line therapy”, “aggregating knowledge and improving awareness”, “clinical effectiveness”, “patients’ preferences”, “reimbursement”, “cost-effectiveness” and “hospital costs”. The factors identified echo those from the literature, but their relevance and interrelationship are case-specific. Besides evidence on clinical effectiveness, contextual factors from 10 other clusters should be addressed to support adoption of MR-HIFU.
Changes in Sedentary Time and Implicit Preference for Sedentary Behaviors in Response to a One-Month Educational Intervention in Primary School Children: Results from the Globe Trotter Pilot Cluster-Randomized Study
Authors: Fillon Alicia , Fearnbach Nicole , Vieira Stéphanie , Gélinier Jade , Bagot Sarah , Bailly Mélina , Boscaro Audrey , Pélissier Léna , Siroux Julie , Grasteau Vincent , Bertsch Jean , Pereira Bruno , Duclos Martine , Lambert Céline , Thivel David
Published: January 2023

School-based multi-component educational interventions have been encouraged to improve children’s movement behaviors. The present study evaluates the effect of the Globe Trotter Initiative on physical activity (PA) level, sedentary time, physical fitness and activity preferences in primary school children. A total of 361 children (9–10 years) participated in this cluster-randomized trial. Nine schools were randomized as control (CON, 121 children) or Globe Trotter schools (GT, 240 children). Physical fitness, body composition, anthropometric characteristics, PA level, sedentary behaviors, physical self-perception, and activity preferences were evaluated at baseline (T0) and after the one-month intervention (T1). Grip strength performance and overall completion time of the obstacle course show a significant time effect (p < 0.001) in both groups (no group effect). PA level and physical self-perception did not significantly show time nor group effects. The sedentary behavior score displays a significant “time × group” interaction effect (p = 0.04) with a significant reduction between T0 and T1 in the GT group only (p < 0.001). The explicit liking for sedentary activities shows a significant “time × group” interaction (p = 0.02) with a significant decrease between T0 and T1 in the GT group only (p < 0.001). The explicit wanting for sedentary activities show a significant “time × group” interaction (p = 0.02) with a significant decrease between T0 and T1 in the GT group only (p < 0.001). The short-term, multi-component, behavioral, educational GT intervention had beneficial effects on primary-school-aged children’s sedentary time and implicit preference for physical over sedentary activities.
Gender Differences in Socio-Demographic Factors Associated with Pre-Frailty in Japanese Rural Community-Dwelling Older Adults: A Cross-Sectional Study
Authors: Shibata Aki , Suzuki Asuka , Takahashi Kenzo
Published: January 2023

Purpose: To prevent frailty, it is necessary to focus on pre-frailty and consider preventive interventions that incorporate social aspects. This study aimed to explore socio-demographic associations with pre-frailty, focusing on modifiable social factors among community-dwelling older adults in a rural Japanese city. Methods: We conducted a self-administered survey on social, physical, and mental factors, and basic attributes, in September 2021. Respondents were classified as frail, pre-frail, or healthy according to their The Kihon Checklist scores. Of the 494 valid responses, 93 respondents classified as pre-frail and 110 as healthy were analyzed. The socio-demographic associations with pre-frailty were investigated by multiple logistic regression, and interactions between gender and other factors were examined. Results: The analysis identified that for both genders, no-community participation in middle age (odds ratio [OR], 1.84; 95% confidence interval [CI], 1.16–2.92) was found to be a social factor associated with pre-frailty. Having friends who listen to one’s concerns (OR, 2.54; 95% CI, 1.26–5.10) was a factor for women. Conclusions: This study showed that modifiable social factors associated with pre-frailty were community involvement and being able to share concerns with friends. The findings suggest the need for support that emphasizes social aspects to prevent pre-frailty.
Can the Policy of Increasing Retirement Age Raise Pension Revenue in China—A Case Study of Anhui Province
Authors: Hu Jin , Stauvermann Peter-Josef , Nepal Surya , Zhou Yuanhua
Published: January 2023

With gradual progress in the medical field and the rising living standard of people, the life expectancy of people is gradually increasing. Unfortunately, this positive development contributes significantly to the aging of societies and creates huge challenges for pension systems. In order to mitigate the pressure on its pension system in the coming years, China is considering increasing the retirement age, just like many other countries. Based on the wage data of urban employees, pension revenue and expenditure data of employees in Anhui Province over the years, we constructed a model to predict average wages and forecast the revenue of the urban pension system from 2022 to 2032. We predicted the pension revenues by simulating an adjusted retirement age under two different schemes. The results of the study showed that the policies of appropriately increasing the retirement age can raise pension revenue. Compared with a one-step retirement age change scheme, a rolling retirement age change scheme that increases the retirement age by several months each year was found to be more suitable for the healthy development of the pension system.
Causal Model Analysis of the Effect of Formalism, Fear of Infection, COVID-19 Stress on Firefighters’ Post-Traumatic Stress Syndrome and Insomnia
Authors: Tang Yun-Ming , Wu Tsung-Lin , Liu Hsiang-Te
Published: January 2023

As the front line of epidemic prevention, firefighters are responsible for the transportation of infected cases. Firefighters are under a lot of stress from the new COVID-19, especially the fear that they may contract the virus at work and spread the virus to their families. In particular, the framework of this study incorporates Riggs’ formalism variables. When firefighters think that the epidemic prevention regulations are inconsistent with the actual epidemic prevention, it will increase their work pressure on COVID-19. In this study, firefighters from all over Taiwan were used as the respondents, and a total of 453 respondents were obtained. This study uses confirmatory factor analysis and structural equation modeling to test the established hypotheses. The findings confirm that formalism, fear of self and family infection are positively influencing COVID-19 stress. COVID-19 stress positively affects PTSD and insomnia. COVID-19 stress negatively affects problem-focused strategies. Problem-focused strategies negatively affect post-traumatic stress disorder.
Challenges, Opportunities, and Coping in the Wake of the COVID-19 Pandemic: The Case of Jewish Communities around the World
Authors: Braun-Lewensohn Orna
Published: January 2023

Against the backdrop of the COVID-19 pandemic, which lasted more than two years and included several waves, the present study focused on Jewish communities around the world, in order to understand the role of community during the pandemic. This study focused on the community mechanisms that helped community members to cope with the pandemic. To that end, between October 2021 and July 2022, in-person interviews were conducted with leaders and members of the following communities: Budapest, Hungary; Subotica, Serbia; Vienna, Austria; Bratislava, Slovakia; Vilna, Lithuania; Buenos Aires, Rosario, Salta, and Ushuaia in Argentina; and Mexico City and Cancun in Mexico. Each interview lasted between 45 min and 1.5 h. All of the interviews were audio-recorded and transcripts of those recordings were prepared. Three major themes emerged from the interviews: challenges, coping, and opportunities. Most of these themes were common to the different communities around the world. The findings of this work are discussed in terms of the concept of sense of community and resiliency theories.
Validation of the Glover–Nilsson Smoking Behavioral Questionnaire (GN-SBQ) to Evaluate Nicotine Dependence in Spanish Clinical Settings
Authors: Carballo José Luis , Rodríguez-Espinosa Sara , Sancho-Domingo Clara , Coloma-Carmona Ainhoa
Published: January 2023

An assessment of the different aspects of tobacco addiction is central to adapting interventions to the profiles and needs of smokers. The Glover–Nilsson Smoking Behavioral Questionnaire (GN-SBQ) is one of the few and most used scales to evaluate the behavioral aspects of tobacco addiction. However, few studies involve the validation of the GN-SBQ in clinical settings. Thus, this study aimed to analyze the psychometric properties of the GN-SBQ in a sample of Spanish smokers. A total of 341 smokers attending clinical services in Spain participated in this cross-sectional study. Measures included the psychological factors related to tobacco addiction, assessed with the GN-SBQ, the physical factors of nicotine addiction, withdrawal symptoms, smoking-related variables, and alcohol use. Data analysis included descriptive statistics, internal consistency coefficients, confirmatory factor analyses, Spearman correlations, and the Kruskal–Wallis test. The GN-SBQ showed adequate reliability (α = 0.76 and ω = 0.76) and a unidimensional structure. GN-SBQ scores also provided evidence of convergent and concurrent validity. GN-SBQ scores significantly correlated with the physical symptoms of addiction, age, number of cigarettes, and withdrawal symptoms. The results of discriminant validity were also adequate, as no correlation was observed between GN-SBQ scores and CO levels or alcohol use. Significant differences were found between all levels of psychological addiction based on the GN-SBQ scores regarding physical nicotine addiction, withdrawal symptoms, and age. Thus, this questionnaire is a reliable and valid instrument to assess the psychological aspects of tobacco addiction in smokers in clinical settings. The short length of the GN-SBQ proves advantageous for its use in time-limited assessments, which are common in public health services.
Weekly External Load Performance Effects on Sports Injuries of Male Professional Football Players
Authors: Martins Francisco , Marques Adilson , França Cíntia , Sarmento Hugo , Henriques Ricardo , Ihle Andreas , de Maio Nascimento Marcelo , Saldanha Carolina , Przednowek Krzysztof , Gouveia Élvio Rúbio
Published: January 2023

One of the most challenging issues professional football players face throughout their careers is injuries. Those injuries often result from suboptimal training programs that were not designed according to the players’ individual needs. This prospective study aimed to examine in detail the effects of sports injuries on professional football players’ weekly external load performances. Thirty-three male professional football players were monitored using 10-Hz Global Positioning System (GPS) units (Apex pro series, StatSports) during an entire season. The variables considered in the analysis were total distance (TD), high-speed running (HSR), accelerations (ACC), and decelerations (DEC). The comparisons were made between the four-week block before injury (–4T), four-week block after return (+4T), and players’ season averages (S). Players displayed significantly higher values of TD, HSR, ACC, and DEC in the –4T, compared to the other two moments (+4T and S). Furthermore, the comparison between the +4T and S showed no significant variations in the GPS metrics. It was shown that a significant increase in players’ weekly external load performance over a four–week period may have a negative effect on the occurrence of injuries from a professional football standpoint. Future research should consider the effects of injury severity on players’ external load variations.
Impact of Physical Activity on Anxiety, Depression, Stress and Quality of Life of the Older People in Brazil
Authors: Figueira Helena Andrade , Figueira Olivia Andrade , Figueira Alan Andrade , Figueira Joana Andrade , Polo-Ledesma Reinaldo Emilio , Lyra da Silva Carlos Roberto , Dantas Estelio Henrique Martin
Published: January 2023

Background: Population aging all over the world invites older people to be active, considering physical activity (PA) as associated with reduced anxiety, depression and stress and a high quality of life (QOL) in older people. Objective: To analyze anxiety, depression, stress and QOL as a function of PA in older people. Methodology: Descriptive analytical research. Six hundred and ninety older people answered the instrument composed of sociodemographic questions, followed by questions from the WHOQOL-Old, Baecke-Old, WHOQOL-SRPB, Stress Perception Scale, Beck Anxiety Inventory and Beck Depression Inventory. Results: The beneficial effect of PA on the elderly is evident in this sample with such a high QoL (73%) and such a high level of physical activity (84%), and even with an advanced level of education (75%) and high spirituality (99.6%). The influence of PA on the anxiety and stress cluster showed Pearson’s chi-square = 9.9, DF = 4, p = 0.04239, critical value = 9.5. The influence of PA on the anxiety, depression and stress cluster showed Pearson’s chi-square = 6.8; DF = 5; p-value = 0.24; critical value = 11.1. Conclusion: In the elderly, PA has a significant relationship with anxiety, stress and QOL. In addition, the high level of QOL of the elderly in the sample demonstrates the capacity for PA, educational level and spirituality for personal satisfaction.
Association between PhA and Physical Performance Variables in Cancer Patients
Authors: Gutiérrez-Santamaría Borja , Martinez Aguirre-Betolaza Aitor , García-Álvarez Arturo , Arietaleanizbeaskoa Maria Soledad , Mendizabal-Gallastegui Nere , Grandes Gonzalo , Castañeda-Babarro Arkaitz , Coca Aitor
Published: January 2023

Maintaining the physical performance of cancer patients is increasingly considered due to the growing number of cancer patients and the aggressiveness of the treatments. For this reason, bioimpedance is now being used to record patients’ body composition by obtaining the phase angle (PhA). Although there is a direct relationship between PhA, age, sex and disease prognosis, it has not been measured as an analysis of physical performance in oncology patients and is a valid tool in the follow-up of cancer patients. For this purpose, 311 patients were evaluated, and both bioelectrical impedance analysis (BIA) and physical performance measurements were performed. The modification of the results concerning PhA was found to be highly relational, as a variation in one of the variables affected the other. It was concluded that each degree increase in PhA modified −22.57 s [−27.58; −17.53] in 400-m walking test (400 mWT); 13.25 kg [10.13; 16.35] in upper-body strength (UBS); 6.3 [4.95; 7.65] in lower-body strength (LBS); 1.55 mL/kg/min [0.98; 2.12] in VO2peak; 6.53 Watts [3.83; 9.20] in ventilatory threshold 1 (VT1); 10.36 Watts [7.02; 13.64] in ventilatory threshold 2 (VT2). It was also noted that age was a factor that affected the relationship between PhA and 400 mWT; the older the age, the higher the relationship. PhA data has been shown to be highly correlated with physical performance. This is of great importance in clinical practice because a cancer patient’s physical performance levels can be assessed during treatment.