bluuebunny/crossref_metadata_2025 huggingface dataset:
Associations of Individual and Combined Physical Activity and Body Mass Index Groups with Proinflammatory Biomarkers among Colorectal Cancer Patients
Authors: Himbert Caroline , Warby Christy A. , Gigic Biljana , Ose Jennifer , Lin Tengda , Viskochil Richard , Peoples Anita R. , Ashworth Anjelica , Schrotz-King Petra , Scaife Courtney L. , Cohan Jessica N. , Jedrzkiewicz Jolanta , Schirmacher Peter , Grady William M. , Cohen Stacey A. , Krane Mukta , Figueiredo Jane C. , Toriola Adetunji T. , Siegel Erin M. , Shibata David , Round June L. , Huang Lyen C. , Li Christopher I. , Schneider Martin , Ulrich Alexis , Hardikar Sheetal , Ulrich Cornelia M.
Published: September 2022

Background:

Physical activity and obesity are well-established factors of colorectal cancer risk and prognosis. Here, we investigate associations of individual and combined physical activity and body mass index (BMI) groups with proinflammatory biomarkers in colorectal cancer patients.

Methods:

Self-reported physical activity levels were classified as “active” (≥8.75 MET-hours/week) versus “inactive” (<8.75 MET-hours/week) in n = 579 stage I–IV colorectal cancer patients enrolled in the ColoCare Study. BMI [normal weight (≥18.5–<25 kg/m2), overweight (≥25–<30 kg/m2), and obese (≥30 kg/m2)] was abstracted from medical records. Patients were classified into four combinations of physical activity levels and BMI. Biomarkers [C-reactive protein (CRP), SAA, IL6, IL8, and TNFα] in presurgery serum samples were measured using the Mesoscale Discovery Platform. Regression models were used to compute relative percent differences in biomarker levels by physical activity and BMI groups.

Results:

“Inactive” patients had non-statistically significant higher IL6 levels compared with “active” patients (+36%, P = 0.10). “Obese” patients had 88% and 17% higher CRP and TNFα levels compared with “normal weight” patients (P = 0.03 and 0.02, respectively). Highest CRP levels were observed among “overweight or obese/inactive” compared with “normal weight/active” patients (P = 0.03).

Conclusions:

We provide evidence of associations between individual and combined physical activity and BMI groups with proinflammatory biomarkers. Although BMI was identified as the key driver of inflammation, biomarker levels were higher among “inactive” patients across BMI groups.

Impact:

This is the largest study in colorectal cancer patients investigating associations of energy balance components with inflammatory biomarkers. Our results suggest that physical activity may reduce obesity-induced inflammation in colorectal cancer patients and support the design of randomized controlled trials testing this hypothesis.
Early Enhancement with Contrast-Enhanced Ultrasonography Relates to the Number of Small-Diameter Neovessels in the Carotid Plaque
Authors: Takeshita Sho , Ogata Toshiyasu , Uesugi Noriko , Nabeshima Kazuki , Shimada Hirofumi , Arima Hisatomi , Inoue Tooru , Tsuboi Yoshio
Published: September 2022

Background and Purpose: Intraplaque neovessels (INVs) have been recognized as a major cause of intraplaque hemorrhage and subsequent vulnerability of the carotid plaque. However, the exact mechanisms by which INVs cause intraplaque hemorrhage remain unclear. Various sizes of INVs coexist in carotid plaques pathologically, and we hypothesized that the size of INVs would be associated with carotid plaque histology, particularly in terms of intraplaque hemorrhage. Detection method of INV is important when determining whether carotid plaques are vulnerable, and contrast-enhanced ultrasonography (CEUS) is one of the most useful methods to detect them. The purpose of this study was to examine the relationship between findings from CEUS and vascular pathology obtained by carotid endarterectomy (CEA). We focused on associations between small and large INVs evaluated by CEUS and histologically defined intraplaque hemorrhage. Methods: Participants comprised 115 patients (mean age, 73.0 ± 7.2 years; 96 men) who underwent preoperative CEUS and underwent CEA. CEUS findings were evaluated as vascular grade at 0 min (Vas-G0) and 10 min (Vas-G10) after contrast injection. Plaques were histologically evaluated quantitatively for the total area of intraplaque hemorrhage, cholesterol, and calcification and the thinnest fibrous cap. Immunohistochemical studies were conducted using anti-CD-34 antibody as a marker for endothelial cells. INVs were divided into two groups depending on diameter: small INVs, <50 μm; and large INVs, ≥50 μm. The numbers of small and large blood vessels in the plaque were quantified histologically. Associations of small and large INVs with CEUS, plaque histology, and clinical findings were assessed by uni- and multivariable analyses. Results: Multivariable analyses indicated that CEUS Vas-G0 was associated with the 4th quartile of the number of small INVs compared with other quartiles, and Vas-G10 was associated with the 4th quartile of the number of large INVs. Histologically, the presence and area of intraplaque hemorrhage were associated with the number of small INVs, while the increased number of large INVs was associated with infrequent plaque disruption and thicker fibrous cap. Conclusions: Our study showed that early phase enhancement in the CEUS can help identify plaque vulnerability by predicting a larger number of small INVs. This information can also help determine treatment strategies for carotid plaque.
Follow-Up of Plasma Donors after Quarantine Storage: Results of a 5-Year Survey in Germany
Authors: Fiedler Sarah Anna , Oberle Doris , Henseler Olaf , Heiden Margarethe , Funk Markus Benedikt
Published: September 2022

Background and Objectives: In 1993, a quarantine storage of 6 months was introduced for plasma for transfusion and was reduced to 4 months in 2003, owing to the improvements of screening assays used in German blood establishments. The presented survey analyses the value of quarantine storage under the current screening conditions. Materials and Methods: From 2015 to 2019, we collected data on the total amount of released quarantine plasma as well as on the number of quarantine plasma not released due to a reactive screening test of a follow-up donation. Results: Responding establishments covered 84% of plasma units released within the sampled period. In 3,583,913 (99.98%) of the total 3,584,664 test pairs, all screening assays revealed a negative result, leading to plasma release from quarantine storage. In 442 out of the residual 751 cases, confirmed positive results for human immunodeficiency virus (24), hepatitis C virus (22), or hepatitis B virus (396) were obtained in the follow-up donations. Of them, 372 revealed negative ID-NAT results in their retain samples confirmed by using highly sensitive individual donor nucleic acid amplification technology. In 70 cases, no testing of retain samples was performed as plasma was released for fractionation. The maximum theoretical risk for an undetected human immunodeficiency virus, hepatitis C or B virus infection was less than 0.0001%. Conclusion: No positive donation were found under the current screening regime and the quarantine storage during the 5-year survey period. In view of the current type and sensitivity of screening tests in German blood establishments, the results allow a reassessment of the value of quarantine storage of plasma regarding duration and release modalities. Due to the more sensitive donor screening, shorter quarantine periods as well as dispensing quarantine storage can be discussed. A reduction in the safety standard of plasma transfusions need not be feared, and the availability of plasma for transfusions could be facilitated.
Biological Difference between L858R and Exon 19 Deletion Contributes to Recurrence-Free Survival of Resected Non-Small Cell Lung Cancer
Authors: Masago Katsuhiro , Kuroda Hiroaki , Fujita Shiro , Sasaki Eiichi , Takahashi Yusuke , Shinohara Shuichi , Matsushita Hirokazu
Published: September 2022

Introduction: The differences in biological characteristics among different genotypes of classical EGFR mutations have not been clarified. This study aimed to clarify the clinical and biological differences between L858R and 19 deletion in NSCLC. Methods: We analyzed a cohort of 191 consecutive cases of surgically resected NSCLC harboring EGFR driver mutations (L858R or 19 deletion) in which curative resection was performed in Aichi Cancer Center Hospital, Nagoya, Japan, from January 2006 to September 2021 and in which recurrence subsequently developed. We also subjected 61 surgically resected NSCLC specimens harboring EGFR driver mutations (L858R or 19 deletion) to an RNA sequencing analysis. Results: In patients with stage I disease, the median time to recurrence did not differ to a statistically significant extent between the types of EGFR mutations; however, among those with stage II and III disease, the median time to recurrence in patients with the L858R genotype tended to be shorter in comparison to those with 19 deletion (log-rank test, p = 0.47 and 0.46, respectively). In comparison to 19 deletion tumors, L858R tumors had higher cytological malignancy (e.g., mitotic ability) and showed stronger immunogenicity. Conclusion: L858R and 19 deletion tumors are likely to have a slight difference in the time to recurrence. They suggest that even in EGFR driver tumors, which are treated as the same disease category, the biological characteristics of the tumors are different, which may leave room for innovations in postoperative treatment and treatment at recurrence.
Autologous Anti-CD19 CAR T-Cells immunotherapy in relapsed/refractory acute lymphoblastic leukemia patients. A systematic review and meta-analysis
Authors: Pasquini Neto Ricardo , Mazzo Felipe Antonio Torres , Bueno Gustavo de Souza , Previdi João Vitor Correa , Silva Nasthia Kreuz Baziulis da , Vieira Tulio da Silva , Magalhães Maria Cristina Figueroa
Published: September 2022

Introduction: Acute Lymphoblastic Leukemia (ALL) is the most prevalent malignancy in children; however, when the neoplasm becomes refractory/relapses (R/R) the cure possibilities are practically null. Objectives: To analyze the Anti-CD19 Chimeric Antigen Receptors (CAR) T-Cells immunotherapy efficacy in the treatment of R/R ALL, providing evidence about the efficacy and safety of the therapy for the analyzed group. Methods: The study consisted of a systematic review and meta-analysis based on the analysis of indexed articles. The searches were carried out with the terms: “acute lymphoblastic leukemia”, “CAR T”, and “CD19-specific chimeric antigen receptor”. Results: Only 18 of the 94 articles obtained initially met the inclusion criteria and were selected for review, totaling 637 patients. Thus, it was observed in the responses that approximately 81% of the patients achieved a Complete Response; 7% did not respond; the neoplasm relapsed in 17% of the cases; and 6.1% of the patients died. The main side effects found were Cytokine Release Syndrome (CRS), Severe Cytokine Release Syndrome, and Neurotoxicity, present in 36.3%, 29%, and 24% of patients, respectively. Conclusion: Anti-CD19 CAR T-Cells immunotherapy is an effective therapy, capable of producing high rates of complete remission in R/R ALL treatment.
Síndrome POEMS: desafios no diagnóstico de um caso atípico
Authors: Silva Rodrigo Rufino Pereira , Martins Mariana Andrade de Figueiredo , Costa Breno Antônio de Albuquerque Lobo , Arruda Talina Tassi Saraiva de , Silva Arilson Santos Alves da , Aguiar Yanne Almeida de , Barros Filho Paulo Bernardo da Silveira
Published: September 2022

Introdução: Síndrome POEMS trata de um raro evento paraneoplásico, sem relato atual na literatura sobre sua real prevalência. A maior parte dos casos ocorre em homens de meia idade. Relato do Caso: Paciente masculino, 65 anos, com queixa edema e parestesia em pernas que evoluiu para plegia, associada a hiporexia e fadiga. Investigação ambulatorial inicial evidenciou Polirradiculoneuropatia Inflamatória Desmielinizante Crônica (PIDC) de etiologia indefinida. Excluídos secundarismos, o paciente foi diagnosticado com hipotireoidismo primário, hipogonadismo severo, lesões hipercrômicas em pele, ascite, derrame pleural e trombocitose, além de gamopatia monoclonal IgA Lambda por imunofixação sérica. Sorologias virais negativas. Excluída a possibilidade de Mieloma Múltiplo e outras gamopatias, foi aventada a hipótese de Síndrome POEMS, sendo realizada dosagem de VEGF plasmática (425 pg/mL; VR = <96.2). Trata-se de um caso atípico na medida em que, lesões ósseas, presentes em até 97% dos casos, não foram evidenciadas no paciente em questão, tornando desafiador o diagnóstico. Conclusões: O diagnóstico de síndromes raras, embora desafiante, traz ao clínico um olhar mais amplo do paciente na medida em que incrementa o raciocínio clínico.
A transformação da percepção e o efeito de “choque” nos filmes da Visual Music: ressonâncias na contemporaneidade
Authors: Hippertt Rebeca Torrezani Martins
Published: September 2022

Este artigo tem como objetivo apresentar relações, distanciamentos e aproximações entre conceitos trabalhados no ensaio “Sobre alguns temas em Baudelaire”, de Walter Benjamin, e a produção fílmica da Visual Music. Busca-se, pois, deste cotejo, destrinchar o tema da transformação da percepção na modernidade, bem como do efeito de choque, à luz do cinema da Visual Music, com destaque especialmente à produção artística de Oskar Fischinger e John Whitney. Tais expressões fílmicas serão usadas aqui como lentes, mediante as quais é possível ver e analisar, a partir de um diálogo com Benjamin – entre o que se ilumina e se obscurece – aspectos da experiência na modernidade e seus impactos na contemporaneidade.
O atraso pede passagem: a longa espera de Paulo Emílio Salles Gomes
Authors: Vigneron Victor Santos
Published: September 2022

O artigo a seguir trata de um pequeno conjunto de críticas escritas entre 1957 e 1959 por Paulo Emílio Salles Gomes. O objetivo é discutir, a partir desse material, uma primeira formulação mais sistemática para o sentimento de frustração do autor diante do cinema nacional. Esse problema seria retomado em seus ensaios mais conhecidos, produzidos no início dos anos 1960, e revela uma trajetória pouco conhecida de hesitações e reflexões em torno do par “expectativa-frustração”. O estudo desses artigos conclui que o atraso emergia então como categoria central para abordar não apenas a produção nacional, mas a própria postura do intelectual diante dela.
Morphology of larval stages of Arrenurus cuspidator (O. F. Müller) and A. maculator (O. F. Müller) (Acari: Hydrachnidia)
Authors: ZAWAL ANDRZEJ
Published: September 2022

The morphology o f the larval stage of A. c uspidator is re-described and A. maculato r is described. Particular attention is paid to character differences between the two species. The present description of larval A. cuspidator is more deta iled than the previously published description. Larva e of both species ar e very simila r and di ffer only in four well de fined cha r acte r s: presence o f a hair b r ush on the base of the C1 seta in A. m aculato r ; the distance s Mp2-Mp2 , Mp1-Lp2 , C1-C2, C1 -CpI medial margin , and C4-CpIII medial ma rgin; th e length of the PV8 seta (longer in A. maculato r ); and the length of the antero-latera l indents in th e dorsa l plate (longer i n A. c uspidato r ). The species are separated from one another mainl y by the shape of dorsal plate with th e relatively deep anterolateral indents into which they cut at a straight ang le.
Ventichthys biospeedoi n. gen. et sp. (Teleostei, Ophidiidae) from a hydrothermal vent in the South East Pacific
Authors: NIELSEN JØRGEN G. , MØLLER PETER R. , SEGONZAC MICHEL
Published: September 2022

A new genus and species of ophidiid fishes, Ventichthys biospeedoi, is described based on two male specimens caught in the French BIOSPEEDO expedition to the South East Pacific Rise. They were caught by a baited trap at the hydrothermal vent site Oasis (17°25.38’S, 113°12.29’W) at 2586 m. The new genus differs from all of the 48 known ophidiid genera by its thick skin, posteriorly placed and enlarged kidneys, broad head, and 4 lateral lines. The most similar genera are Benthocometes and Petrotyx having a blunt snout, one basibranchial tooth patch, granular teeth, and no free pectoral fin rays. Remarks on the ecology of the new species are based on analysis of video documents taken in situ by a submarine Nautile.
A new species of the weevil genus Larinus Dejean from Turkey and Syria (Coleoptera: Curculionidae: Lixinae)
Authors: GÜLTEKİN LEVENT
Published: September 2022

Larinus perrinae sp. n. is described from southeastern Turkey and Syria based on material from J. Desbrochers’ and M. Pic’s collections. Lectotype of L. lejeunei Capiomont is designated. Larinus perrinae is closely related to L. lejeunei but, differing in the elongated body, pattern of pubescence and shape of aedeagus.
Understanding saproxylic beetles: new records of Tetratomidae, Melandryidae, Synchroidae, and Scraptiidae from the Maritime Provinces of Canada (Coleoptera: Tenebrionoidea)
Authors: MAJKA CHRISTOPHER G. , POLLOCK DARREN A.
Published: September 2022

New species records for Tetratomidae, Melandryidae, Synchroidae, and Scraptiidae from the Maritime Provinces of Canada are given. Known bionomics of these saproxylic species are summarized and new bionomic observations are provided. Twenty-five species are newly recorded from Nova Scotia, 10 from Prince Edward Island, and three from New Brunswick. Thirty-seven species are now known from the region, a substantial increase from the 23 species known hitherto. Range extensions for 19 species are noted. The composition of the region’s fauna is briefly discussed, focusing on a disparity in the collection efforts between provinces, and also on island faunas. Finally the fauna is examined in the context of a developing knowledge of saproxylic beetles in Nova Scotia, along with potential concerns about the impact that forest management practices.
A review of the millipede genus Dorypetalum Verhoeff, 1900 (Diplopoda: Callipodida: Dorypetalidae)
Authors: STOEV PAVEL , ENGHOFF HENRIK
Published: September 2022

A new millipede species, Dorypetalum helenae (Callipodida: Dorypetalidae), is described from the Kuru Dağ Mts, European Turkey. The species is morphologically close to D. bulgaricum from the Rhodope Mts, SE Bulgaria but is well distinguished by the particular, racket-shaped mesal coxosternal process of gonopods. All hitherto described species in the genus are reviewed, and new illustrations are provided for D. bosniense and D. trispiculigerum. A new key for identification of the species in the genus is made to accommodate the new findings.
A new species of Ptychognathus Stimpson, 1858 (Crustacea: Decapoda: Brachyura: Varunidae) from the Ryukyu Islands, southwestern Japan
Authors: OSAWA MASAYUKI , NG NGANKEE
Published: September 2022

A new varunid crab, Ptychognathus insolitus, is described from Izena Island, the Ryukyu Islands, southwestern Japan. This new species is unique among congeners in having a very broad carapace, long and slender ambulatory legs, and stout male first gonopods. Although these characters are diagnostic to Orcovita Ng & Tomascik, 1994, the new species belongs to Ptychognathus Stimpson, 1858 based on the lack of a distinct frontal median triangle on the carapace and the short dactyli of the fifth pereopods.
A new species of Coelopleurus (Echinodermata: Echinoidea: Arbaciidae) from New Caledonia
Authors: COPPARD SIMON E. , SCHULTZ HEINKE A.G.
Published: September 2022

Coelopleurus exquisitus sp. nov. Coppard & Schultz, 2006 occurs at depths of 240 m to 520 m off of New Caledonia in the South Pacific. This new species is distinctive in having large naked interambulacral median regions that are purple with an undulating lavender line, in conjunction with highly curved primary spines that are banded red and pale-green on their dorsal surface for three quarters of the distal length, pointed secondary spines and aboral ophicephalous pedicellariae that have constricted valves.
Revised diagnosis of the genus Bangana Hamilton, 1822 (Pisces: Cyprinidae), with taxonomic and nomenclatural notes on the Chinese species
Authors: ZHANG E. , CHEN Y.-Y.
Published: September 2022

Most morphological characters diagnostic of the 13 Chinese species of the cyprinid genus Sinilabeo Rendahl, 1932, are identical to those of the genus Bangana Hamilton, 1822. Consequently, these 13 species are transferred to Bangana. A revised diagnosis is provided for the now-expanded genus Bangana, and a dichotomous key and taxonomic and nomenclatural notes are included for the following valid Chinese species: B. decora, B. dero, B. devdevi, B. discognathoides, B. lemassoni, B. lippa, B. rendahli, B. tonkinensis, B. tungting, B. wui, B. xanthogenys, B. yunnanensis, and B. zhui. Literature reports, by Chinese authors, of Sinilabeo dero from the upper Irrawady River basin, in Yunnan, are based on misidentifcations of B. devdevi. Sinilabeo cirrhinoides Wu and Lin in Wu, Lin, Chen, Chen and He, 1977, and S. laticeps Wu and Lin in Wu, Lin, Chen, Chen and He, 1977, are junior subjective synonyms of B. devdevi and B. lippa, respectively. Sinilabeo yunnanensis Wu, Lin, Chen, Chen and He, 1977, is an available name, and a lectotype is designated for the species. Bangana zhui (Zheng and Chen, 1983) is a valid species distinct from B. yunnanensis.
Two new species of Kapateira Young from Costa Rica (Auchenorrhyncha: Cicadellidae: Cicadellinae)
Authors: GODOY CAROLINA , GARITA-CAMBRONERO JERSON , RIVERA CARMEN , VILLALOBOS WILLIAM
Published: September 2022

The genus Kapateira was described by Young (1977), who included one species from Colombia but noted that the genus also occurs in Panama, Venezuela, Eucador, and Bolivia. In this paper two new species are decribed, K. peruana from Peru and K. coffea from Costa Rica. The latter species is associated with coffee and citrus, and information is provided on its biology. DAS-ELISA tests for the bacterium, Xylella fastidiosa, yielded numerous positive results, suggesting that K. coffea is a potential vector of diseases such as coffee leaf scorch and citrus variegation chlorosis.
New palearctic species of the bee genus Andrena (Insecta: Hymenoptera: Andrenidae)
Authors: DUBITZKY ANDREAS
Published: September 2022

Eight palearctic species of seven different subgenera of the bee genus Andrena are described as new for science: A. (Carandrena) planti sp. n. from Turkmenistan, A. (Euandrena) yangi sp. n. and A. (Habromelissa) nantouensis sp. n. from Taiwan, A. (Larandrena) susanneae sp. n. from China, A. (Leucandrena) cheni sp. n. from Taiwan, A. (Pallandrena) christineae sp. n. from Turkey and Iran, A. (Pallandrena) scheuchli sp. n. from Turkmenistan and A. (Simandrena) heinzi sp. n. from Kazakhstan and Kyrgyzstan. Morphological characters of the new species are documented by SEM.
The taxonomic status of Phrynopus pereger Lynch 1975 (Amphibia, Anura, Leptodactylidae)
Authors: LEHR EDGAR , GUILAR CÉSAR
Published: September 2022

Live and freshly preserved specimens of Phrynopus pereger have terminal discs with a small, papilla-like projection and weak, interrupted circumferential grooves separating the disc from a pad. Based on external morphological and osteological characters Phrynopus pereger is transferred to Eleutherodactylus. Information on its natural history and distribution, and photos in life are provided. All Peruvian Phrynopus (except P. pereger) occur at elevations between 2600 and 4400 m.
A synopsis of the fleas (Insecta: Siphonaptera) parasitizing New World species of Soricidae (Mammalia: Insectivora)
Authors: MORRONE JUAN J. , ACOSTA ROXANA
Published: September 2022

A synopsis of the 57 species and 24 genera of New World fleas parasitizing species of Soricidae is provided, with synonymical lists, and distributional and host data. Species analyzed belong to the families Ceratophyllidae (10 genera), Ctenophthalmidae (10 genera), Hystrichopsyllidae (two genera), and Leptopsyllidae (two genera). Three subspecies are elevated to species: Corrodopsylla lira, C. obtusata, and Nearctopsylla hygini. Soricid hosts include 17 species of Sorex, four species of Cryptotis, two species of Blarina, and one species of Crocidura. The species hosting the highest number of flea species is Blarina brevicauda (19 flea species), followed by Sorex cinereus (16), S. pacificus (9), S. trowbridgii (8), S. fumeus (7), S. palustris (7), and Cryptotis parva (7). Most of the flea species analyzed are polyxenous, and belong mainly to genera associated to small rodents and marginally present on Soricidae. The association with Soricidae seems to be more evident in species of Corrodopsylla, Corypsylla, Ctenophthalmus, Doratopsylla, Hystrichopsylla,and Nearctopsylla. Only Corrodopsylla lira, C. barrerai, Ctenophthalmus cryptotis, C. expansus, C. myodosus, Hystrichopsylla cryptotis, H. guatemalensis, Nearctopsylla georgiana, N. pfitzeri, and N. princei are apparently stenoxenous or monoxenous. Taxa analyzed belong to the Nearctic biotic element and are specially diversified in the Mexican Transition Zone.
Two new species of Quadrimaera (Crustacea: Amphipoda: Melitidae) from Atol das Rocas, Brazil
Authors: SENNA ANDRÉ R. , SEREJO CRISTIANA S.
Published: September 2022

A field collection of Crustacea was made in the Biological Reserve of Atol das Rocas, Northeastern Brazil, from October 2000 to October 2001. Specimens were obtained from washed algae, from the inner and outer parts of the annular reef between 0–20 m depths. In this paper we describe two new species of the genus Quadrimaera Krapp-Schickel & Ruffo, 2000 (Amphipoda: Melitidae) from Atol das Rocas. Quadrimaera chaelata sp. nov. differs from all other species of the genus by a strong process in the palmar corner on the propodus of gnathopod 2 that essentially makes it chelate; Quadrimaera rocasensis sp. nov. differs from its congeners in size, shape, and order of excavations on the palm of gnathopod 2, by a robust spine on the inner margin of the dactylus of gnathopod 2 that opposes the first excavation of the palm, and by three long bifid setae and a nail with an apical seta, in each lobe of the telson. The two new species are endemic to Atol das Rocas. This study raises to three the number of known species of the genus Quadrimaera from the atoll: the two new species and Quadrimaera cristianae Krapp-Schickel & Ruffo, 2000. A key to identification for the species of Quadrimaera from Atol das Rocas is given.
New Zealand species of the shore-fly genus Nostima Coquillett(Diptera: Ephydridae)
Authors: EDMISTON JAMES F. , MATHIS WAYNE N.
Published: September 2022

The shore-fly genus Nostima Coquillett is first reported from New Zealand in a revision of the three species now known to occur there. Species included are: Nostima kiwistriata sp. n., N. negramaculata sp. n. and N. duoseta Cresson. The latter species was first described from Australian specimens and is here reported to occur widely in New Zealand. Detailed locality data from New Zealand are provided for each species. The phylogenetic relationships between the New Zealand species and species from other geographical regions are presented. A key to the species of Nostima occurring in New Zealand is presented.
Genetic identification and color descriptions of early life-history stages of Belizean Phaeoptyx and Astrapogon (Teleostei: Apogonidae) with Comments on identification of adult Phaeoptyx
Authors: BALDWIN CAROLE C. , MOUNTS JULIE H. , SMITH DAVID G. , WEIGT LEE A.
Published: September 2022

Phaeoptyx and Astrapogon are represented in the Caribbean by six species (P. conklini, P. pigmentaria, P. xenus, A. alutus, A. stellatus, and A. puncticulatus). Species identification of larvae and juveniles is problematic because characters used to distinguish adults (e.g., patterns of pigmentation and numbers of gill rakers) are absent, incomplete, or difficult to discern in the young stages. Neighbor-joining trees derived from mitochondrial cytochrome oxidase 1 sequences (DNA Barcoding) were used to match early life stages and adults. Subsequent comparative analysis of preserved voucher specimens from which the DNA was extracted or digital color photographs of those specimens taken prior to preservation yielded sufficient information to separate all early life-history stages of Belizean Phaeoptyx and Astrapogon and provided additional information for field identification of adult Phaeoptyx. Patterns of chromatophores in fresh material, combined with patterns of melanophores, provide the easiest means of separating the life-history stages of Phaeoptyx. Larvae of Astrapogon species are morphologically very similar, and some differences in pigmentation detected among them may reflect different stages of development. Continued implementation of the DNA Barcoding methods and field protocol outlined herein should prove valuable in accurately identifying much more of the ichthyoplankton fauna of the Caribbean.
Further specimens and phylogenetic position of the recently described leaf turtle species Cyclemys gemeli (Testudines: Geoemydidae)
Authors: PRASCHAG PETER , HUNDSDÖRFER ANNA K. , FRITZ UWE
Published: September 2022

We describe external morphology and habitat of ten specimens of Cyclemys gemeli, a recently discovered leaf turtle species from north-eastern India, previously known only from its incomplete holotype and photos of a live female. Further, we assess the phylogenetic position of C. gemeli using sequence data of the mitochondrial cytochrome b gene as well as of three nuclear DNA fragments (C-mos, Rag2 genes, intron 1 of R35 gene) and confirm its genetic distinctiveness. Mitochondrial data strongly suggest a sister group relationship of C. gemeli and C. fusca, another species occurring in Myanmar. According to our new records, the Naga Hills and the Arakan Mts could constitute the geographical divide between C. gemeli and C. fusca. Morphologically, C. gemeli resembles other dark-bellied Cyclemys species and determination by external morphology alone is quite difficult.
A new species of Pareas (Serpentes: Colubridae: Pareatinae) from the Gaoligong Mountains, southwestern China
Authors: GUO KEJI , DENG XUEJIA
Published: September 2022

A new species of Pareas Wagler, 1830 (Serpentes: Colubridae: Pareatinae) is described from Gaoligong Mountains, Yunnan province, southwestern China. The new species is distinguished from congeners in the following combination of traits: preoculars 1/1; subocular and postocular fused into a single crescent-shaped scale; anterior temporals 1, posterior 2–3; 15 dorsal rows on whole body, 9 median rows feebly keeled (invisible on neck); vertebral scales slightly enlarged; ventrals 175; subcaudals 76 (excluding terminal spine), all divided; a big black oval patch on the back of head; two round black spots on each side of the face.
A new brooding feather star of the genus Dorometra (Echinodermata: Crinoidea: Comatulida: Antedonidae) from the Ryukyu Islands, southwestern Japan
Authors: OBUCHI MASAMI , KOGO ICHIZO , FUJITA YOSHIHISA
Published: September 2022

A new comatulid of the family Antedonidae, Dorometra sesokonis n. sp., is described from coral reefs of the Ryukyu Islands, southwestern Japan. The new species is extremely small with ten short arms and a unique pinnulation in which some proximal pinnules are absent or poorly developed in most specimens. Additionally, cirrals and brachials of D. sesokonis are elongated and partly colorless. The combination of these morphological characters makes the new species appear like juvenile specimens, regardless of sexual maturity. This species also has remarkable reproductive habits, exhibiting both external brooding and hermaphroditism.
Geographic distribution of the genus Pleurodema in Argentina (Anura: Leiuperidae)
Authors: FERRARO DAIANA P. , CASAGRANDA M. DOLORES
Published: September 2022

Distributional ranges of South American frogs are usually poorly known. In this paper, we examined 4671 specimens of the genus Pleurodema deposited in Argentinean herpetological collections and used these museum data to generate distributional maps for the nine species of the genus currently known in Argentina (P. cf. borellii, P. bufoninum, P. cf. cinereum, P. kriegi, P. guayapae, P. marmoratum, P. nebulosum, P. thaul and P. tucumanum). Then, we contrasted the distributional ranges of each species with ecological regions proposed for the country. The species of the genus Pleurodema are widely distributed along northwestern, central, western, and southern Argentina from sea level up to 4275 m a.s.l., and all of them occur at protected areas of the country. We listed localities where two or more species live in sympatry. We found a correlation between point-occurrence data and distance to main roads of the country, where 50% of specimens were collected at a distance minor than 2 km from main roads. Finally, we discussed doubtful records of Pleurodema species taken from both museum records and literature on the basis of our knowledge about the distributional range of these species.
Morphological comparisons of the Indo-West Pacific scorpionfish, Parascorpaena aurita, with a closely related species, P. picta, with first records of P. aurita from East Asia (Scorpaeniformes: Scorpaenidae)
Authors: MOTOMURA HIROYUKI , SAKURAI YOU , SENOU HIROSHI , HO HSUAN-CHING
Published: September 2022

The poorly known scorpionfish Parascorpaena aurita is redescribed on the basis of 96 specimens from the Indo-Pacific and compared in detail with a closely related species, P. picta. The two species can be distinguished from other congeners by lacking a suborbital spine below the eye and having usually 17 or 18 pectoral-fin rays. Examination of a large number of specimens of each species revealed that P. aurita differs from P. picta in a number of scale counts and morphometrics, and morphology of the suborbital and interorbital ridges, and occiput. Previously published photographs and/or specimen lists identified as P. picta included both species. Parascorpaena aurita is widely distributed in the IndoPacific, including East Asia (first records), whereas P. picta is restricted to Southeast Asia and Australia.
An updated checklist of the scale insects (Hemiptera: Coccoidea) of the Margarodidae sensu lato group
Authors: BEN-DOV Y.
Published: September 2022

This article is an update (closing-date December 2010) of the Margarodidae sensu lato catalogue (Ben-Dov, 2005a). The 457 species and subspecies placed among 73 genera of scale insects are classified in 11 families. The geographical distribution and host plants are given for each species and subspecies.
Description of the pupa and male of Paryphoconus grandis Macfie (Diptera: Ceratopogonidae)
Authors: RONDEROS MARÍA M. , MARINO PABLO I. , DÍAZ FLORENTINA
Published: September 2022

The pupa and male of Paryphoconus grandis Macfie are described for the first time. Pupae were collected from the edgeof a stream margin in Corrientes province, Argentina and reared to adults. Pupae and adults are illustrated and photographed. The species is known from Mexico to northern Argentina.
A complex of Transversotrematidae (Platyhelminthes: Digenea) associated with mullid fishes of the Indo-West Pacific Region, including the descriptions of four new species of Transversotrema
Authors: HUNTER JANET A. , HALL KATHRYN A. , CRIBB THOMAS H.
Published: September 2022

Phylogenetic analysis based on ribosomal DNA markers indicates that there is a discrete clade of species of Transverso-trema Witenberg, 1944 associated with mullid fishes of the Indo-West Pacific, although there is only one previous recordof transversotrematids from these fishes. Each of the species in this clade bears broad morphological similarity to previousconcepts of either Transversotrema haasi Witenberg, 1944 or Transversotrema licinum Manter, 1970, yet none of them isattributed here to either of these species. Four new species of Transversotrema are described from mullid fishes of thetropical and sub-tropical Indo-West Pacific using a combination of morphology, morphometric analysis and DNA se-quencing. Transversotrema cabrarum n. sp. is described from Parupeneus spilurus (Bleeker) from Ningaloo Reef (Aus-tralia), and recorded from Parupeneus cyclostomus (Lacepède), Parupeneus pleurostigma (Bennett) and Parupeneustrifasciatus (Lacepède) from the same location. Transversotrema chevrarum n. sp. is described from Lizard Island (GreatBarrier Reef, Australia) from Parupeneus ciliatus (Lacepède), and further recorded from Parupeneus barberinus(Lacepède), P. cyclostomus, Parupeneus indicus (Shaw), Parupeneus multifasciatus (Quoy & Gaimard) and P. trifasciatusat the same location. Transversotrema cutmorei n. sp. is described from Upeneus tragula Richardson from Moreton Bay,Australia. Transversotrema tragorum n. sp. is described from Parupeneus indicus from Lizard Island and recorded fromP. barberinus, P. ciliatus P. cyclostomus, P. multifasciatus and P. trifasciatus from the same location. An additional spe-cies, also from mullids from Lizard Island, is delineated on the basis of molecular data, but not formally described. Like many other transversotrematids, the species described here appear to have limited geographical distributions.
Thalasseleotrididae, new family of marine gobioid fishes from New Zealand and temperate Australia, with a revised definition of its sister taxon, the Gobiidae (Teleostei: Acanthomorpha)
Authors: GILL ANTHONY C. , MOOI RANDALL D.
Published: September 2022

Thalasseleotrididae n. fam. is erected to include two marine genera, Thalasseleotris Hoese & Larson from temperate Aus-tralia and New Zealand, and Grahamichthys Whitley from New Zealand. Both had been previously classified in the familyEleotrididae. The Thalasseleotrididae is demonstrably monophyletic on the basis of a single synapomorphy: membraneconnecting the hyoid arch to ceratobranchial 1 broad, extending most of the length of ceratobranchial 1 (= first gill slitrestricted or closed). The family represents the sister group of a newly diagnosed Gobiidae on the basis of five synapo-morphies: interhyal with cup-shaped lateral structure for articulation with preopercle; laterally directed posterior processon the posterior ceratohyal supporting the interhyal; pharyngobranchial 4 absent; dorsal postcleithrum absent; urohyalwithout ventral shelf. The Gobiidae is defined by three synapomorphies: five branchiostegal rays; expanded and medially-placed ventral process on ceratobranchial 5; dorsal hemitrich of pelvic-fin rays with complex proximal head. This studyrepresents a contribution to our ongoing clarification of the family Eleotrididae, which has served historically as a repository for genera not classified among the more derived gobioids (= Gobiidae as defined here).
A new species of the hermit crab genus Ciliopagurus Forest (Crustacea: Decapoda: Anomura: Diogenidae) from southern India
Authors: KOMAI TOMOYUKI , RESHMI REMA , KUMAR APPUKUTTAN NAIR BIJU
Published: September 2022

The diogenid hermit crab genus Ciliopagurus Forest, 1995 is currently represented by 18 recent species, 17 species fromthe Indo-West Pacific and one species from the eastern Atlantic. In this paper, we describe a new species, Ciliopagurusgrandis, from off Kerala State, southern India. This new species is compared with six congeneric species, C. albatrossiForest, 1995, C. caparti (Forest, 1952), C. krempfi (Forest, 1952), C. liui Forest, 1995, C. macrolepis Forest, 1995, and C. shebae (Lewinsohn, 1969). It is characteristic in having weakly armed antennal acicles.
Fabrication of Quercitrin Nano Micellar Delivery System and Its Therapeutic Effect on Unexplained Recurrent Abortion
Authors: Lu Lidan , Cai Ximei , Guo Luqin , Ji Hongjian , Ren Jiajie , Ni Haiyan , Feng Xiaoling
Published: September 2022

We prepared a quercitrin nano micellar delivery system in this study to evaluate its oral bioavailability. The optimal formulation of quercetin nano micelles was determined through an orthogonal test. Characteristics (size of particles-SOP, morphology, efficiency of encapsulation-EE
and stability) and the therapeutic property of quercitrin nano micelles on unexplained recurrent abortion (URSA) were evaluated. The SOP of quercitrin nano micelles was 111.88±3.70 nm with an EE of 95.66±0.57. A substantially increased release rate of quercetin from the micellar
system was observed in different dissolution media comparable to that of quercitrin. Also, through quercitrin micelles, the oral bioavailability of quercetin was increased by 15.45-fold compared to quercitrin solution. Significantly, quercetin could reduce the levels of LDH and SOD as well
as increase the level of MDA in serum restricted HTR-8/SVneo cells. Western blotting (WB) experiments showed that quercitrin had a protective effect on H2O2 induced oxidative stress injury of a human placental trophoblast HTR8-SVneo cell line. The developed nano micelles
are a potential carrier that could enhance the aqueous solubility, oral in vivo availability and potential therapeutic abortion effect of quercitrin.
An Artificial Small RNA Editor by Chimeric dsRNase with RNA Binding Protein
Authors: Li Danhua , Guo Fangfang , Yue Hongfang , Huang Yaqi , Lu Chenchen , Guo Yubai , Liu Qinghua , Li Yanqiang
Published: September 2022

RNA plays a vital role in cell functions, but tools to manipulate it is limited. RNA interference (RNAi) is an important approach for biological and clinical applications, but the prone of non-target knockdown effects limited the usage. CRISPR-Cas13 systems recently have been identified
for RNA-guided RNA-interfering activity, and can be used in therapeutics, but the large size of Cas13 proteins and the off-targets effect also limit their further usage. Here we report that the chimeric protein containing a double strand nuclease/domain and a structure RNA binding domain (dsRNase-stRBD)
with structure guided RNA (sgRNA) can be engineered for mammalian RNA silencing effectively. The RNA knockdown mediated by this method was durable, efficient and stringent without off-target interfering by the sense strand of shRNA base method. Moreover, at size of only 307 aa, allowing dsRNase-stRBD
fitting for the versatile scAAV, while the most recent report displays that the smallest Cas13 protein is 775 aa. These results establish sgRNA-dsRBD-RNase as an excellent method for studying RNA function of cells and further clinical application.
Delivery of Chondrocyte-Targeting sh-TTTY15 (Testis-Specific Transcript, Y-Linked 15) Nanoparticles Against LncRNA TTTY15 Improves Osteoarthritis by Regulating Autophagy and Oxidative Stress
Authors: Yi Yangfei , Yang Ningyin , Luo Qian , Tao Xiaojun , Li Yufei
Published: September 2022

Objective: To explore the potential role of hyaluronic acid-modified peptide-lncRNA TTTY15 nanoparticles in joint injury of chondrocytes in OA rats. Methods: Cell proliferation, apoptosis and oxidative stress were tested by CCK8, flow cytometry and biochemical analysis.
Histopathology and LC3 expression were analyzed by HE, TUNEL and IF. The expression levels of TTTY15, LC3, p62, c-caspase3, Col2A1, ACAN, ADAMTS-5 and MMP13 were tested by RT–qPCR, western blotting and IHC. Autophagosomes were observed by TEM. Results: Bioinformatics and RT–PCR
analyses showed that TTTY15 was highly expressed in OA- and TBHP-stimulated chondrocytes. Ov-TTTY15 aggravated TBHP-induced activity decreases, apoptosis, oxidative stress, ECM degradation and autophagic flux reduction in chondrocytes. HA-coated-p5RHH-sh-TTTY15 nanoparticle intervention enhanced
the stability and prolonged TTTY15 silencing in chondrocytes. HA-coated-p5RHH-sh-TTTY15 nanoparticles inhibited TBHP-induced C-28/I2 cell damage and activated autophagy, and the inhibitory effect was greater than that of sh-TTTY15. Conclusion: HA-coated-p5RHH-sh-TTTY15 nanoparticles
enhanced the stable silencing of TTTY15 in chondrocytes; promoted cell proliferation; inhibited apoptosis, oxidative stress and ECM degradation; and activated autophagy to improve joint injury in OA rats.
Persimmon Tannin-Reduction Graphene Oxide-Platinum-Palladium Nanocomposite Decorated on Screen-Printed Carbon Electrode for Enhanced Electrocatalytic Reduction of Hydrogen Peroxide
Authors: Li Guiyin , Xue Yewei , Wang Chaoxian , Li Xinhao , Li Shengnan , Huang Yong , Zhou Zhide
Published: September 2022

According to studies, Hydrogen peroxide (H2O2) is a significant biomarker of physiological processes. Unnormal H2O2 levels in human body may result in diseases. Hence, there is an increasing demand for monitoring the H2O2
concentrations in biological specimen. Here, we construct a non-enzymatic H2O2 electrochemical biosensor based on persimmon tannin-reduced graphene oxide-platinum-palladium nanocomposite (PrG-Pt@Pd NPs) modified with screen-printed carbon electrode (SPE). Combined with
suitable electrocatalytic mode for Pt@Pd NPs, high specific large specific volume and good electrical conductivity of RGO, well as the superior sorption capacity of PT for metal-based nano-ion, the PrGPt@Pd striped pleasing heterogeneous catalytic activity toward H2O2
reduction via the synergistic effect. In experimental conditions of optimal, this non-enzymatic electrochemical sensor exhibited excellent electrocatalytic performance for H2O2 with less negative potential (−0.5 V), fast response time (<3 s), it shows good linearity
in the range of 5.0–100.0 μM, in addition to this LOD of this sensor was 0.059 μM as well as the excellent sensitivity of the sensor (13.696 μA·μM−1·cm−2). Due to excellent specificity, lower detection
limit, and good recovery (98.70–99.96%) in the spiked measurements of human serum samples, this non-enzymatic electrochemical biosensor paves the way for H2O2 detection at ultra-low concentrations in physiology and diagnosis.
A Research on the Role and Mechanism of N-Methyl-D-Aspartate Receptors in the Effects of Silver Nanoparticles on the Electrical Excitability of Hippocampal Neuronal Networks
Authors: Hou Kun , Meng Chen , Huang Yan , Zhang Zequn , Wang Zhigong , Lü Xiaoying
Published: September 2022

The purpose of this paper is to explore the role and mechanism of N-Methyl-D-Aspartate (NMDA) receptors in the effects of silver nanoparticles (SNPs) on the electrical excitability of hippocampal neuronal networks. First, the cytotoxicity of different concentrations of SNPs was evaluated
and screened by MTT experiment, then the Voltage Threshold Measurement Method (VTMM) was employed to study the effects of SNPs on the electrical excitability of hippocampal neuronal networks under non-cytotoxic (5 μM) and cytotoxic (100 μM) concentrations after different
action times. The role of NMDA receptors in the effects of SNPs on the electrical excitability of hippocampal neuronal networks was investigated through the NMDA receptor antagonist MK-801. Then, the effects of SNPs on the number of NMDA receptors and the Ca2+ content in hippocampal
neurons were further investigated, and the relationship between these changes and neuronal networks electrical excitability was discussed. The results of voltage threshold (VTh) test showed that non-cytotoxic 5 μM SNPs has an excitatory effect on hippocampal neuronal
networks, while the effect of cytotoxic 100 μM SNPs gradually changed from excitatory to inhibitory with the extension of action time. It was found that SNPs could increase the electrical excitability of neuronal networks by activating NMDA receptors through the experiments with
MK-801 antagonists. Moreover, the fluorescent staining experiments showed that the activation of NMDA receptors by SNPs can lead to an increase in the intracellular Ca2+ content, and then trigger a negative feedback regulation mechanism of neurons between the number of NMDA receptors
and intracellular Ca2+ content. The high Ca2+ content in neurons can also decrease neurons’ cell viability, which in turn leads to changes in the electrical excitability of the neuronal networks.