bluuebunny/crossref_metadata_2025 huggingface dataset:
Antimicrobial Photodynamic Therapy Combined With Antibiotic in the Treatment of Rats With Third-Degree Burns
Authors: Zhao Zhanjuan , Ma Jinduo , Wang Yiyi , Xu Zehua , Zhao Lu , Zhao Jianxi , Hong Ge , Liu Tianjun
Published: February 2021

Cationic porphyrin conjugate, protoporphyrin IX-methyl ethylenediamine derivative (PPIX-MED) has a potent photosensitive antibacterial effect on clinically isolated bacteria, including methicillin-resistant Staphylococcus aureus, (MRSA), Escherichia coli, and Pseudomonas aeruginosa. This study investigated (i) the PPIX-MED-mediated antimicrobial photodynamic effect on these three species in vitro and (ii) the effect of antimicrobial photodynamic therapy (aPDT) combined with the use of an antibiotic on the healing in vivo of third-degree burns of rats with the wounds infected by these bacterial species. PPIX-MED exerted a potent inhibitory effect on the growth of the three bacterial species by producing reactive oxygen species when photoactivated. PPIX-MED-mediated antimicrobial photodynamic therapy (PPIX-MED-aPDT) had high bacterial photoinactivation ability in vitro, with a minimum inhibitory concentration of 15.6 μM PPIX-MED against each of the three types of bacteria and minimum bactericidal concentrations of 31.25 μM against MRSA and E. coli and 62.5 μM against P. aeruginosa. In rats with third-degree burns infected by a mixture of these bacteria, the bactericidal efficiency of PPIX-MED–aPDT-combined-with-antibiotic treatment was higher than that of antibiotic or aPDT treatment alone. This was confirmed by analysis of viable bacterial counts in wound tissue and blood. Enzyme-linked immunosorbent assay revealed that aPDT-combined-with-antibiotic treatment resulted in an obvious reduction in tumor necrosis factor-alpha and interleukin-6 levels compared with the no-treatment control group and the other treatment groups. Immunohistochemistry revealed that the expression of basic fibroblast growth factor and CD31 (a marker of neovascularization), expressed in burn wound tissue was higher in the aPDT-combined-with-antibiotic treatment group than in the other groups. PPIX-MED–aPDT has a promising bactericidal effect both in vitro and in vivo, and PPIX-MED–aPDT-combined-with-antibiotic treatment enhanced the healing of infected third-degree burns in rats.
Cognitive Impairment in Adolescent Major Depressive Disorder With Nonsuicidal Self-Injury: Evidence Based on Multi-indicator ERPs
Authors: Wen Yujiao , Zhang Xuemin , Xu Yifan , Qiao Dan , Guo Shanshan , Sun Ning , Yang Chunxia , Han Min , Liu Zhifen
Published: February 2021

The lifetime prevalence of major depressive disorder (MDD) in adolescents is reported to be as high as 20%; thus, MDD constitutes a significant social and public health burden. MDD is often associated with nonsuicidal self-injury (NSSI) behavior, but the contributing factors including cognitive function have not been investigated in detail. To this end, the present study evaluated cognitive impairment and psychosocial factors in associated with MDD with NSSI behavior. Eighteen and 21 drug-naïve patients with first-episode MDD with or without NSSI (NSSI+/– group) and 24 healthy control subjects (HC) were enrolled in the study. The Hamilton Anxiety Scale (HAMA), Hamilton Depression Scale (HAMD), Adolescent Self-injury Questionnaire, Beck Scale for Suicide Ideation–Chinese Version (BSI-CV), Shame Scale for Middle School Students, Sensation Seeking Scale (SSS) and Childhood Trauma Questionnaire (CTQ) were used to assess depression-related behaviors, and event-related potentials (ERPs) were recorded as a measure of cognitive function. The latency of the N1, N2, P3a, P3b, and P50 components of ERPs at the Cz electrode point; P50 amplitude and P50 inhibition (S1/S2) showed significant differences between the 3 groups. CTQ scores also differed across three groups, and the NSSI– and NSSI+ groups showed significant differences in scores on the Shame Scale for Middle School Students. Thus, cognitive function was impaired in adolescents with MDD with NSSI behavior, which was mainly manifested as memory decline, attention and executive function deficits, and low anti-interference ability. We also found that childhood abuse, lack of social support, and a sense of shame contributed to NSSI behavior. These findings provide insight into the risk factors for MDD with NSSI behavior, which can help mental health workers more effectively diagnose and treat these patients.
Stimulation and Recording of the Hippocampus Using the Same Pt-Ir Coated Microelectrodes
Authors: Elyahoodayan Sahar , Jiang Wenxuan , Lee Curtis D. , Shao Xiecheng , Weiland Gregory , Whalen John J. , Petrossians Artin , Song Dong
Published: February 2021

Same-electrode stimulation and recording with high spatial resolution, signal quality, and power efficiency is highly desirable in neuroscience and neural engineering. High spatial resolution and signal-to-noise ratio is necessary for obtaining unitary activities and delivering focal stimulations. Power efficiency is critical for battery-operated implantable neural interfaces. This study demonstrates the capability of recording single units as well as evoked potentials in response to a wide range of electrochemically safe stimulation pulses through high-resolution microelectrodes coated with co-deposition of Pt-Ir. It also compares signal-to-noise ratio, single unit activity, and power efficiencies between Pt-Ir coated and uncoated microelectrodes. To enable stimulation and recording with the same microelectrodes, microelectrode arrays were treated with electrodeposited platinum-iridium coating (EPIC) and tested in the CA1 cell body layer of rat hippocampi. The electrodes’ ability to (1) inject a large range of electrochemically reversable stimulation pulses to the tissue, and (2) record evoked potentials and single unit activities were quantitively assessed over an acute time period. Compared to uncoated electrodes, EPIC electrodes recorded signals with higher signal-to-noise ratios (coated: 9.77 ± 1.95 dB; uncoated: 1.95 ± 0.40 dB) and generated lower voltages (coated: 100 mV; uncoated: 650 mV) for a given stimulus (5 μA). The improved performance corresponded to lower energy consumptions and electrochemically safe stimulation above 5 μA (>0.38 mC/cm2), which enabled elicitation of field excitatory post synaptic potentials and population spikes. Spontaneous single unit activities were also modulated by varying stimulation intensities and monitored through the same electrodes. This work represents an example of stimulation and recording single unit activities from the same microelectrode, which provides a powerful tool for monitoring and manipulating neural circuits at the single neuron level.
Bioactive Composition and Free Radical Scavenging Activity of Fresh Orange Maize Hybrids: Impacts of Genotype, Maturity Stages, and Processing Methods
Authors: Alamu Emmanuel Oladeji , Maziya-Dixon Bussie , Menkir Abebe , Irondi Emmanuel Anyachukwu , Olaofe Olorunfemi
Published: February 2021

Bioactive compounds in foods are responsible for their biological activities, but biotic and abiotic factors may influence their levels. This study evaluated the impact of three genotypes (designated 4, 5, and 7), maturity stages (20, 27, and 34 days after pollination) and processing methods (hydrothermal and dry-heating) on the bioactive constituents (carotenoids, phytate, tannins, vitamin C) and 2,2-diphenyl-2-picrylhydrazyl radical (DPPH*) scavenging activity of fresh orange maize hybrids. Freshly harvested maize cobs of each genotype were subjected to hydrothermal processing at 100°C and dry-heating with husks and without husks. Carotenoids (lutein, zeaxanthin, β-cryptoxanthin, α-carotene, and total β-carotene) contents of fresh and processed samples were analyzed using HPLC; other bioactive constituents and DPPH* scavenging ability were determined using spectrophotometric methods. Genotype had a significant effect on the levels of carotenoids (p < 0.001) and vitamin C (p < 0.05), while genotype (p < 0.001), and processing methods (p < 0.001) had significant effects on DPPH* SC50. Maturity stages, processing methods and their interaction also had significant effects (p < 0.001) on the levels of all the bioactive constituents. A positive moderate to strong correlation was observed between (p < 0.001) α-carotene and the following: lutein (r = 0.57), β-cryptoxanthin (r = 0.69), total β-carotene (r = 0.62). However, the relationship between α-carotene and zeaxanthin was positive but weak (r = 0.39). A positive moderate correlation (p < 0.001) was observed between lutein and the following: β-cryptoxanthin (r = 0.57), total β-carotene (r = 0.58), and zeaxanthin (r = 0.52). A positive strong correlation (p < 0.001) was observed between β-cryptoxanthin and each of total β-carotene (r = 0.92) and zeaxanthin (r = 0.63); total β-carotene and zeaxanthin (r = 0.65); while the association between vitamin C and DPPH* SC50 was negative and weak (r = −0.38). Generally, genotype 4 and harvesting at 34 days after pollination had the best combination of bioactive constituents and DPPH* scavenging ability.
Impact of Graft Weight Change During Perfusion on Hepatocellular Carcinoma Recurrence After Living Donor Liver Transplantation
Authors: Kim Jong Man , Chung Young Jae , Kim Sangjin , Rhu Jinsoo , Choi Gyu-Seong , Joh Jae-Won
Published: February 2021

Backgrounds

Inadequate liver volume and weight is a major source of morbidity and mortality after adult living donor liver transplantation (LDLT). The purpose of our study was to investigate HCC recurrence, graft failure, and patient survival according to change in right liver graft weight after histidine-tryptophan-ketoglutarate (HTK) solution perfusion in LDLT.

Methods

Two hundred twenty-eight patients underwent LDLT between 2013 and 2017. We calculated the change in graft weight by subtracting pre-perfusion graft weight from post-perfusion graft weight. Patients with increased graft weight were defined as the positive group, and patients with decreased graft weight were defined as the negative group.

Results

After excluding patients who did not meet study criteria, 148 patients underwent right or extended right hepatectomy. The negative group included 89 patients (60.1%), and the positive group included 59 patients (39.9%). Median graft weight change was -28 g (range; -132–0 g) in the negative group and 21 g (range; 1–63 g) in the positive group (P<0.001). Median hospitalization time was longer for the positive group than the negative group (27 days vs. 23 days; P=0.048). There were no statistical differences in tumor characteristics, postoperative complications, early allograft dysfunction, or acute rejection between the two groups. Disease-free survival, death-censored graft survival, and patient survival were lower in the positive group than the negative group. Additionally, the positive group showed strong association with HCC recurrence, death-censored graft survival, and patient survival in multivariate analysis.

Conclusion

This study suggests that positive graft weight change during HTK solution perfusion indicates poor prognosis in LDLT.
lncRNA MIR22HG-Derived miR-22-5p Enhances the Radiosensitivity of Hepatocellular Carcinoma by Increasing Histone Acetylation Through the Inhibition of HDAC2 Activity
Authors: Jin Qiao , Hu Hao , Yan Siqi , Jin Long , Pan Yuliang , Li Xiangjun , Peng Yayi , Cao Peiguo
Published: February 2021

Background

With the development of radiotherapy technology, radiotherapy has been increasingly used to treat primary hepatocellular carcinoma (HCC). However, due to radioresistance and the intolerance of the adjacent organs to radiation, the effects of radiotherapy are often unsatisfactory. Therefore, it is necessary to study radiosensitization in HCC.

Method

A microarray was used to analyze the genes that were significantly associated with radiosensitivity. HCC cells, HepG2 and MHCC97H, were subjected to radiation in vitro. Real-time PCR was performed to determine MIR22HG (microRNA22 host gene) and miR-22-5p expression levels. Western blotting was performed to determine histone expression levels. A histone deacetylase (HDAC) whole cell assay was used to determine the activity of HDAC2. MTT, colony formation, 5-ethynyl-2′-deoxyuridine, and wound healing assays were performed to examine the function of MIR22HG and miR-22-5p in cellular radiosensitivity. Chromatin immunoprecipitation-PCR was used to confirm that HDAC2 affects the acetylation level of the MIR22HG promoter region. Finally, animal experiments were performed to demonstrate the in vivo effect of MIR22HG on the radiosensitivity of hepatoma.

Results

Irradiation can up-regulate MIR22HG expression and down-regulate HDAC2 expression. Inhibition of HDAC2 expression promotes histone acetylation in the MIR22HG promoter region and up-regulates MIR22HG expression. MIR22HG can increase radiosensitivity via miR-22-5p in HCC.

Conclusion

Inhibition of HDAC2 expression promotes histone acetylation in the MIR22HG promoter region, thereby up-regulating the expression of MIR22HG and promoting the production of miR-22-5p, and ultimately increasing the sensitivity of liver cancer radiotherapy.
m6A Modifications Play Crucial Roles in Glial Cell Development and Brain Tumorigenesis
Authors: Wang Jing , Sha Yongqiang , Sun Tao
Published: February 2021

RNA methylation is a reversible post-transcriptional modification to RNA and has a significant impact on numerous biological processes. N6-methyladenosine (m6A) is known as one of the most common types of eukaryotic mRNA methylation modifications, and exists in a wide variety of organisms, including viruses, yeast, plants, mice, and humans. Widespread and dynamic m6A methylation is identified in distinct developmental stages in the brain, and controls development of neural stem cells and their differentiation into neurons, glial cells such as oligodendrocytes and astrocytes. Here we summarize recent advances in our understanding of RNA methylation regulation in brain development, neurogenesis, gliogenesis, and its dysregulation in brain tumors. This review will highlight biological roles of RNA methylation in development and function of neurons and glial cells, and provide insights into brain tumor formation, and diagnostic and treatment strategies.
Sophoridine Inhibits the Tumour Growth of Non-Small Lung Cancer by Inducing Macrophages M1 Polarisation via MAPK-Mediated Inflammatory Pathway
Authors: Zhao Bei , Hui Xiaodan , Zeng Hairong , Yin Yinan , Huang Jian , Tang Qingfeng , Ge Guangbo , Lei Tao
Published: February 2021

Lung cancer is one of the most common and lethal neoplasms for which very few efficacious treatments are currently available. M1-like polarised tumour-associated macrophages (TAMs) are key mediators to modulate the tumour microenvironment, which play a key role in inhibiting cancer cell growth. Sophoridine, a naturally occurring alkaloid, exerts multiple pharmacological activities including anti-tumour and anti-inflammatory activities, but it has not been characterised as a regulator of tumour microenvironment towards NSCLC. Herein, the regulatory effects of sophoridine on the polarisation of THP-1 cells into TAMs and the anti-tumour effects of sophoridine-stimulated M1 polarised macrophages towards lung cancer cells were carefully investigated both in vitro and in vivo. The results showed that sophoridine could significantly promote M1 polarisation of RAW264.7 and THP-1-derived macrophages, leading to increased expression of pro-inflammatory cytokines and the M1 surface markers CD86 via activating MAPKs signaling pathway. Further investigations showed that sophoridine-stimulated RAW264.7 and THP-1-derived M1 macrophages effectively induced cell apoptosis as well as inhibited the cell colony formation and cell proliferation in both H460 and Lewis lung cancer cells. In Lewis-bearing mice model, sophoridine (15 or 25 mg/kg) significantly inhibited the tumour growth and up-regulated the expression of CD86/F4/80 in tumour tissues. Collectively, the findings clearly demonstrate that sophoridine promoted M1-like polarisation in vitro and in vivo, suggesting that sophoridine held a great therapeutic potential for treating lung cancer.
Case Report: Two Infant Cases of Langerhans Cell Histiocytosis Involving the Digestive Tract
Authors: Wang Huan , Wang Yuesheng , Wang Ruifeng , Li Xiaoqin
Published: February 2021

Langerhans cell histiocytosis (LCH) is a rare disease with uncertain etiology. Langerhans cell histiocytosis with involvement of the gastrointestinal tract is rare and is typically identified in pediatric patients with systemic disease. The present study reports two infantile cases of LCH who initially presented with diarrhea, hematochezia, and rash and were histologically missed on the original examination of the colonic biopsy sections. The diagnosis of LCH was later verified through immunohistochemistry. By combining our experience and previous reports, the multiple hemorrhagic spots of the colorectal mucosa and narrowness and erosion of the distal duodenum might be suggestive manifestations of gastrointestinal involvement in LCH on endoscopic examination. This might be helpful for the early recognition of the disease.
The Combination of Aquilaria sinensis (Lour.) Gilg and Aucklandia costus Falc. Volatile Oils Exerts Antidepressant Effects in a CUMS-Induced Rat Model by Regulating the HPA Axis and Levels of Neurotransmitters
Authors: Li Huiting , Li Yuanhui , Zhang Xiaofei , Ren Guilin , Wang Liangfeng , Li Jianzhe , Wang Mengxue , Ren Tao , Zhao Yi , Yang Ming , Huang Xiaoying
Published: February 2021

The Aquilaria sinensis (Lour.) Gilg (CX)–Aucklandia costus Falc. (MX) herbal pair is frequently used in traditional Chinese medicine prescriptions for treating depression. The volatile oil from CX and MX has been shown to have good pharmacological activities on the central nervous system, but its curative effect and mechanism in the treatment of depression are unclear. Therefore, the antidepressant effect of the volatile oil from CX–MX (CMVO) was studied in chronic unpredictable mild stress (CUMS) rats. The suppressive effects of CMVO (25, 50, 100 μL/kg) against CUMS-induced depression-like behavior were evaluated using the forced swimming test (FST), open field test (OFT) and sucrose preference test (SPT). The results showed that CMVO exhibited an antidepressant effect, reversed the decreased sugar preference in the SPT and prolongation of immobility time in the FST induced by CUMS, increased the average speed, time to enter the central area, total moving distance, and enhanced the willingness of rats to explore the environment in the OFT. Inhalational administration of CMVO decreased levels of adrenocorticotropic hormone and corticosterone in serum and the expression of corticotropin-releasing hormone mRNA in the hypothalamus, which indicated regulation of over-activation of the hypothalamic–pituitary–adrenal (HPA) axis. In addition, CMVO restored levels of 5-hydroxytryptamine (5-HT), dopamine, norepinephrine and acetylcholine in the hippocampus. The RT-PCR and immunohistochemistry results showed that CMVO up-regulated the expression of 5-HT1A mRNA. This study demonstrated the antidepressant effect of CMVO in CUMS rats, which was possibly mediated via modulation of monoamine and cholinergic neurotransmitters and regulation of the HPA axis.
Quantum Gravity: A Fluctuating Point of View
Authors: Pawlowski Jan M. , Reichert Manuel
Published: February 2021

In this contribution, we discuss the asymptotic safety scenario for quantum gravity with a functional renormalization group approach that disentangles dynamical metric fluctuations from the background metric. We review the state of the art in pure gravity and general gravity–matter systems. This includes the discussion of results on the existence and properties of the asymptotically safe ultraviolet fixed point, full ultraviolet-infrared trajectories with classical gravity in the infrared, and the curvature dependence of couplings also in gravity–matter systems. The results in gravity–matter systems concern the ultraviolet stability of the fixed point and the dominance of gravity fluctuations in minimally coupled gravity–matter systems. Furthermore, we discuss important physics properties such as locality of the theory, diffeomorphism invariance, background independence, unitarity, and access to observables, as well as open challenges.
Actively Driven Fluctuations in a Fibrin Network
Authors: Hu Qingda , Morris Tessa Altair , Grosberg Anna , Levine Alex J. , Botvinick Elliot L.
Published: February 2021

Understanding force propagation through the fibrous extracellular matrix can elucidate how cells interact mechanically with their surrounding tissue. Presumably, due to elastic nonlinearities of the constituent filaments and their random connection topology, force propagation in fiber networks is quite complex, and the basic problem of force propagation in structurally heterogeneous networks remains unsolved. We report on a new technique to detect displacements through such networks in response to a localized force, using a fibrin hydrogel as an example. By studying the displacements of fibers surrounding a two-micron bead that is driven sinusoidally by optical tweezers, we develop maps of displacements in the network. Fiber movement is measured by fluorescence intensity fluctuations recorded by a laser scanning confocal microscope. We find that the Fourier magnitude of these intensity fluctuations at the drive frequency identifies fibers that are mechanically coupled to the driven bead. By examining the phase relation between the drive and the displacements, we show that the fiber displacements are, indeed, due to elastic couplings within the network. Both the Fourier magnitude and phase depend on the direction of the drive force, such that displacements typically propagate farther, but not exclusively, along the drive direction. This technique may be used to characterize the local mechanical response in 3-D tissue cultures, and to address fundamental questions about force propagation within fiber networks.
A Review of Tunable Electromagnetic Metamaterials With Anisotropic Liquid Crystals
Authors: Xu Jing , Yang Ruisheng , Fan Yuancheng , Fu Quanhong , Zhang Fuli
Published: February 2021

The performance of metamaterial is limited to a designed narrow band due to its resonant nature, it is highly desirable to incorporate active inclusions in metamaterials to extend the operation bandwidth. This review summarizes the development in realizing the tunability of electromagnetic response in metamaterials incorporated with nematic liquid crystal (LC). From rigorous comparison, it is found that the anisotropic property of nematic LC is essential in predicting the influence of LC molecular director orientation on the resonant frequency of metamaterials. By carefully designing the metamaterials and properly infiltrating LC, the operation frequency of single/double negative parameters of metamaterials can be dynamically modulated with remarkable red/blue-shift, depending on the LC molecular orientation angle. Moreover, the recent liquid crystal-based developments and novel applications are investigated and highlighted.
Predictive Value of the Age, Creatinine, and Ejection Fraction (ACEF) Score in Patients With Acute Fulminant Myocarditis
Authors: Liu Lin , Yang Xinyu , Gu Yiyu , Jiang Tingbo , Xu Jialiang , Xu Mingzhu
Published: February 2021

Objective

Patients with acute fulminant myocarditis often have more adverse cardiovascular events and higher mortality. The purpose of this study was to evaluate the usefulness of age, creatinine, and left ventricular ejection fraction (ACEF score), in determining the risk that acute fulminant myocarditis will lead to serious cardiovascular events, death, and cardiac dysfunction.

Methods

We retrospectively reviewed the demographics, laboratory tests, medications, echocardiographic examinations, in-hospital clinical outcomes, major adverse cardiovascular events (MACE), and survival rate at 1 year in the medical records of 220 consecutive subjects suffering from acute fulminant myocarditis from January 2013 to June 2019.

Results

Two hundred twenty patients were divided into a survivor group and a non-survivor group. This study found that patients in the non-survivor group were older, had higher heart rates, and had more serious injuries to multiple organ functions. A high ACEF score at admission was independently associated with an unfavorable prognosis, and it was a predictor of in-hospital mortality. The current analysis extends the predictive performance of the ACEF scores at 30 days by evaluating echocardiographic data as applied to survivors of fulminant myocarditis and cumulative rates of MACE at 1 year. The results indicated that patients with high ACEF scores had poor recovery of cardiac function, and higher rates of MACE, all-cause death, and heart failure at 1 year than the low-ACEF group.

Conclusion

The ACEF score was identified as an effective predictor of poor in-hospital outcomes, worse cardiac recovery after 30 days, and higher rates of MACE, all-cause death, and heart failure at 1 year in patients who had acute fulminant myocarditis. These data suggest that its predictive accuracy means the ACEF score could be used to assess the prognosis of patients with acute fulminant myocarditis.
The Impact of Matching to Psychotherapy Preference on Engagement in a Randomized Controlled Trial for Patients With Advanced Cancer
Authors: Marziliano Allison , Applebaum Allison , Moyer Anne , Pessin Hayley , Rosenfeld Barry , Breitbart William
Published: February 2021

Objective: This study examined whether patients who were randomly assigned to their preferred therapy arm had stronger engagement with their treatment than those who were randomly assigned to a non-preferred therapy arm.

Method: Data were drawn from a RCT comparing Individual Meaning-Centered Psychotherapy (IMCP), with Individual Supportive Psychotherapy (ISP), in patients with advanced cancer. Treatment engagement was operationalized as patients' perceptions of the therapeutic alliance with their therapist and therapy sessions attended. Two 2 by 2 Analysis of Variance (ANOVA) models were used, with treatment preference (IMCP vs. ISP) and treatment assignment (IMCP vs. ISP) as the independent variables and working alliance and number of sessions attended as outcome variables.

Results: Patients who preferred and were assigned to IMCP reported a significantly stronger alliance than those who preferred IMCP but were assigned to ISP.

Conclusions: The findings from this study have broader implications for research on psychotherapy beyond the appeal of IMCP in advanced cancer patients. Patients who prefer a novel psychotherapy that they cannot engage in elsewhere, but receive the standard treatment may experience weaker alliance than patients who prefer the standard but receive the novel therapy.

Trial registration:Clinicaltrial.gov ID: NCT01323309
Effect of Low Protein Diets with Amino Acids Supplementation on Growth Performance, Carcass Traits, Blood Parameters and Muscle Amino Acids Profile in Broiler Chickens under High Ambient Temperature
Authors: Saleh Ahmed A. , Amber Khairy A. , Soliman Mohamed M. , Soliman Mahmoud Y. , Morsy Wael A. , Shukry Mustafa , Alzawqari Mohammed H.
Published: February 2021

This study evaluates low protein diets with amino acid supplement on growth, biochemical markers and muscle amino acids profile in broilers under high ambient temperature. A total of 480 one-day-old chicks were allocated into three treatments with four replicates (n = 40). Control fed optimal protein and optimal amino acids which contains 23% and 21% crude protein (CP) with 65% methionine + cysteine/ lysine (Met + Cys/Lys) and 55% threonine/lysine (Thr/Lys), LPOA (low protein and optimal amino acids) which contains 21% and 19% CP with 65% Met + Cys/Lys and 55% Thr/Lys and 3. LPHA (low protein and high amino acids) which contains 21 and 19% CP with 74% Met + Cys/Lys and 67% Thr/Lys, respectively during the summer months (The temperatures 32 ± 3 °C, and the relative humidity 72.5% ± 4). Birds fed LPOA diets significantly highest body weight, while those fed LPHA recorded significantly the lowest body weight (BW). Dressing percentages not revealed significantly affected by reducing dietary protein levels, while the blood plasma total protein, albumin, and globulin were not significant differences due to dietary low protein. Chicks fed LPHA diets recorded the highest liver content of malonaldehyde. It could be concluded that feeding the Cobb 500 broilers on low protein diets with the same amino acid levels had no adverse effect on growth, carcass markers, and liver function, however increased amino acids levels to low protein diets may led negative impacts for the broiler performance under high ambient temperature.
Impact of the Sub-Grid Scale Turbulence Model in Aeroacoustic Simulation of Human Voice
Authors: Lasota Martin , Šidlof Petr , Kaltenbacher Manfred , Schoder Stefan
Published: February 2021

In an aeroacoustic simulation of human voice production, the effect of the sub-grid scale (SGS) model on the acoustic spectrum was investigated. In the first step, incompressible airflow in a 3D model of larynx with vocal folds undergoing prescribed two-degree-of-freedom oscillation was simulated by laminar and Large-Eddy Simulations (LES), using the One-Equation and Wall-Adaptive Local-Eddy (WALE) SGS models. Second, the aeroacoustic sources and the sound propagation in a domain composed of the larynx and vocal tract were computed by the Perturbed Convective Wave Equation (PCWE) for vowels [u:] and [i:]. The results show that the SGS model has a significant impact not only on the flow field, but also on the spectrum of the sound sampled 1 cm downstream of the lips. With the WALE model, which is known to handle the near-wall and high-shear regions more precisely, the simulations predict significantly higher peak volumetric flow rates of air than those of the One-Equation model, only slightly lower than the laminar simulation. The usage of the WALE SGS model also results in higher sound pressure levels of the higher harmonic frequencies.
Exploring the Data Efficiency of Cross-Lingual Post-Training in Pretrained Language Models
Authors: Lee Chanhee , Yang Kisu , Whang Taesun , Park Chanjun , Matteson Andrew , Lim Heuiseok
Published: February 2021

Language model pretraining is an effective method for improving the performance of downstream natural language processing tasks. Even though language modeling is unsupervised and thus collecting data for it is relatively less expensive, it is still a challenging process for languages with limited resources. This results in great technological disparity between high- and low-resource languages for numerous downstream natural language processing tasks. In this paper, we aim to make this technology more accessible by enabling data efficient training of pretrained language models. It is achieved by formulating language modeling of low-resource languages as a domain adaptation task using transformer-based language models pretrained on corpora of high-resource languages. Our novel cross-lingual post-training approach selectively reuses parameters of the language model trained on a high-resource language and post-trains them while learning language-specific parameters in the low-resource language. We also propose implicit translation layers that can learn linguistic differences between languages at a sequence level. To evaluate our method, we post-train a RoBERTa model pretrained in English and conduct a case study for the Korean language. Quantitative results from intrinsic and extrinsic evaluations show that our method outperforms several massively multilingual and monolingual pretrained language models in most settings and improves the data efficiency by a factor of up to 32 compared to monolingual training.
Non-Invasive Prediction of Site-Specific Coronary Atherosclerotic Plaque Progression using Lipidomics, Blood Flow, and LDL Transport Modeling
Authors: Sakellarios Antonis I. , Tsompou Panagiota , Kigka Vassiliki , Siogkas Panagiotis , Kyriakidis Savvas , Tachos Nikolaos , Karanasiou Georgia , Scholte Arthur , Clemente Alberto , Neglia Danilo , Parodi Oberdan , Knuuti Juhani , Michalis Lampros K. , Pelosi Gualtiero , Rocchiccioli Silvia , Fotiadis Dimitrios I.
Published: February 2021

Background: coronary computed tomography angiography (CCTA) is a first line non-invasive imaging modality for detection of coronary atherosclerosis. Computational modeling with lipidomics analysis can be used for prediction of coronary atherosclerotic plaque progression. Methods: 187 patients (480 vessels) with stable coronary artery disease (CAD) undergoing CCTA scan at baseline and after 6.2 ± 1.4 years were selected from the SMARTool clinical study cohort (Clinicaltrial.gov Identifiers NCT04448691) according to a computed tomography (CT) scan image quality suitable for three-dimensional (3D) reconstruction of coronary arteries and the absence of implanted coronary stents. Clinical and biohumoral data were collected, and plasma lipidomics analysis was performed. Blood flow and low-density lipoprotein (LDL) transport were modeled using patient-specific data to estimate endothelial shear stress (ESS) and LDL accumulation based on a previously developed methodology. Additionally, non-invasive Fractional Flow Reserve (FFR) was calculated (SmartFFR). Plaque progression was defined as significant change of at least two of the morphological metrics: lumen area, plaque area, plaque burden. Results: a multi-parametric predictive model, including traditional risk factors, plasma lipids, 3D imaging parameters, and computational data demonstrated 88% accuracy to predict site-specific plaque progression, outperforming current computational models. Conclusions: Low ESS and LDL accumulation, estimated by computational modeling of CCTA imaging, can be used to predict site-specific progression of coronary atherosclerotic plaques.
Fungal X-Intrinsic Protein Aquaporin from Trichoderma atroviride: Structural and Functional Considerations
Authors: Amira Maroua Ben , Faize Mohamed , Karlsson Magnus , Dubey Mukesh , Frąc Magdalena , Panek Jacek , Fumanal Boris , Gousset-Dupont Aurélie , Julien Jean-Louis , Chaar Hatem , Auguin Daniel , Mom Robin , Label Philippe , Venisse Jean-Stéphane
Published: February 2021

The major intrinsic protein (MIP) superfamily is a key part of the fungal transmembrane transport network. It facilitates the transport of water and low molecular weight solutes across biomembranes. The fungal uncharacterized X-Intrinsic Protein (XIP) subfamily includes the full protein diversity of MIP. Their biological functions still remain fully hypothetical. The aim of this study is still to deepen the diversity and the structure of the XIP subfamily in light of the MIP counterparts—the aquaporins (AQPs) and aquaglyceroporins (AQGPs)—and to describe for the first time their function in the development, biomass accumulation, and mycoparasitic aptitudes of the fungal bioagent Trichoderma atroviride. The fungus-XIP clade, with one member (TriatXIP), is one of the three clades of MIPs that make up the diversity of T. atroviride MIPs, along with the AQPs (three members) and the AQGPs (three members). TriatXIP resembles those of strict aquaporins, predicting water diffusion and possibly other small polar solutes due to particularly wider ar/R constriction with a Lysine substitution at the LE2 position. The XIP loss of function in ∆TriatXIP mutants slightly delays biomass accumulation but does not impact mycoparasitic activities. ∆TriatMIP forms colonies similar to wild type; however, the hyphae are slightly thinner and colonies produce rare chlamydospores in PDA and specific media, most of which are relatively small and exhibit abnormal morphologies. To better understand the molecular causes of these deviant phenotypes, a wide-metabolic survey of the ∆TriatXIPs demonstrates that the delayed growth kinetic, correlated to a decrease in respiration rate, is caused by perturbations in the pentose phosphate pathway. Furthermore, the null expression of the XIP gene strongly impacts the expression of four expressed MIP-encoding genes of T. atroviride, a plausible compensating effect which safeguards the physiological integrity and life cycle of the fungus. This paper offers an overview of the fungal XIP family in the biocontrol agent T. atroviride which will be useful for further functional analysis of this particular MIP subfamily in vegetative growth and the environmental stress response in fungi. Ultimately, these findings have implications for the ecophysiology of Trichoderma spp. in natural, agronomic, and industrial systems.
Study on Policy Tasks for Promoting a Business Using Spent Electric Vehicle Batteries
Authors: Park Chankook , Kim Jae-Kyung
Published: February 2021

With the rising demand for electric vehicles (EVs), it is also becoming increasingly important for policymakers to devise measures on how best to handle spent EV batteries. Policymakers need to devise a system that ensures the economic efficiency of recycling used EV batteries, and support the effective operation of such a system with legal and policy measures. This study aims to present, in a systematic manner, the policy measures needed to foster an environment that actively makes use of spent EV batteries, based on a review of Korean practices and experiences. Based on in-depth interviews with experts, this study explains why the policy measures it recommends are essential, what obstacles stand in the way of implementing those measures and how we may overcome them, as well as other factors we should consider when implementing these measures. As a result, we identified top-priority policy tasks, such as defining regulatory provisions that apply to each stage using spent batteries, as well as clarifying the cost- and profit-sharing structure. We also explored potential and major obstacles and factors that may stand in the way of the implementation of recommended policy measures.
Neural Network Approach for Global Solar Irradiance Prediction at Extremely Short-Time-Intervals Using Particle Swarm Optimization Algorithm
Authors: Aljanad Ahmed , Tan Nadia M. L. , Agelidis Vassilios G. , Shareef Hussain
Published: February 2021

Hourly global solar irradiance (GSR) data are required for sizing, planning, and modeling of solar photovoltaic farms. However, operating and controlling such farms exposed to varying environmental conditions, such as fast passing clouds, necessitates GSR data to be available for very short time intervals. Classical backpropagation neural networks do not perform satisfactorily when predicting parameters within short intervals. This paper proposes a hybrid backpropagation neural networks based on particle swarm optimization. The particle swarm algorithm is used as an optimization algorithm within the backpropagation neural networks to optimize the number of hidden layers and neurons used and its learning rate. The proposed model can be used as a reliable model in predicting changes in the solar irradiance during short time interval in tropical regions such as Malaysia and other regions. Actual global solar irradiance data of 5-s and 1-min intervals, recorded by weather stations, are applied to train and test the proposed algorithm. Moreover, to ensure the adaptability and robustness of the proposed technique, two different cases are evaluated using 1-day and 3-days profiles, for two different time intervals of 1-min and 5-s each. A set of statistical error indices have been introduced to evaluate the performance of the proposed algorithm. From the results obtained, the 3-days profile’s performance evaluation of the BPNN-PSO are 1.7078 of RMSE, 0.7537 of MAE, 0.0292 of MSE, and 31.4348 of MAPE (%), at 5-s time interval, where the obtained results of 1-min interval are 0.6566 of RMSE, 0.2754 of MAE, 0.0043 of MSE, and 1.4732 of MAPE (%). The results revealed that proposed model outperformed the standalone backpropagation neural networks method in predicting global solar irradiance values for extremely short-time intervals. In addition to that, the proposed model exhibited high level of predictability compared to other existing models.
Effects of Voltage Interharmonics on Cage Induction Motors
Authors: Gnaciński Piotr , Hallmann Damian , Klimczak Piotr , Muc Adam , Pepliński Marcin
Published: February 2021

In a power system, the voltage waveform usually contains harmonics and sometimes interharmonics, often defined as components of frequency greater than the fundamental voltage component but not of its integer multiple. Previous studies have reported a minor effect of voltage interharmonics on a cage induction machine. This paper reveals their extraordinary harmfulness for induction motors. Namely, voltage interharmonics may cause high vibration, which can result in machine damage. In addition, interharmonics can lead to torque pulsations corresponding to the natural frequency of the first elastic mode. Consequently, possible torsional resonance may cause destruction of a power train. In this study, the results of investigations on undesirable phenomena due to interharmonics are presented for seven motors with a rated power 3 kW–5.6 MW.
Survey of CO2 Radiation Experimental Data in Relation with Planetary Entry
Authors: Reynier Philippe
Published: February 2021

This paper focuses on a survey of experimental data related to radiation into CO2 plasma flows, which are encountered during Mars and Venus entries. The review emphasizes on VUV and IR radiation, since recent experimental efforts has been devoted to these wavelength ranges since they contribute mostly to CO2 plasma radiation. The main objective of the study is to identify the most attractive datasets for future crosscheck comparisons with the results obtained during future test campaigns with ESTHER shock-tube. The survey accounts for the results obtained in shock-tubes, expansion tube and plasma arc-jets for Mars and Venus test campaigns. The experimental results obtained for propulsion related studies have also been considered.
Emerging from Below? Understanding the Livelihood Trajectories of Smallholder Livestock Farmers in Eastern Cape Province, South Africa
Authors: Gwiriri Lovemore C. , Bennett James , Mapiye Cletos , Burbi Sara
Published: February 2021

In the context of current agrarian reform efforts in South Africa, this paper analyses the livelihood trajectories of ‘emergent’ farmers in Eastern Cape Province. We apply a rural livelihoods framework to 60 emergent cattle farmers to understand the different capitals they have drawn upon in transitioning to their current class positions and associated vulnerability. The analysis shows that, for the majority of farmers, no real ‘transition’ from subsistence farming has occurred. However, they draw limited resilience from increased livestock holdings, continued reliance on social grants and connections with communal villages. A transition into small-scale commercial farming is apparent for a small number of farmers through the deployment of financial, human and social capitals. However, in following these trajectories, most of these farmers have been made more vulnerable to shocks and stresses than previously. We suggest that key to mitigating this vulnerability will be access to low-risk financial capital, more targeted support, and strategies to support farmers that might not transition from subsistence production.
Input Issues in the Development of L2 French Morphosyntax
Authors: Thomas Anita
Published: February 2021

The aim of this article is to discuss the role of input characteristics in the development of French verb morphology. From a usage-based perspective, several cognitive and linguistic factors contribute to the ease or difficulty of processing input in L2 acquisition. This article concentrates on frequency, salience, and form–function association, factors that might influence what aspects of input are available to the learners’ attention. A presentation of French verb morphology from this perspective shows how these factors can contribute to the use of the regular -er verb paradigm as a default. A review of empirical studies confirms the influence of input characteristics. The results suggest that the dominant pattern of regular verbs and the scarcity of salient clues from irregular verbs contribute to the specificity of L2 French development. The conclusion addresses the question of enriching L2 classroom input with irregular verbs. Such an input could facilitate the perception of form–function association, and thus, contribute to a more efficient development of French verb morphology. The article concludes by suggesting other ways of studying the influence of input as well as avenues for future research.
Existence and Uniqueness of BVPs Defined on Semi-Infinite Intervals: Insight from the Iterative Transformation Method
Authors: Fazio Riccardo
Published: February 2021

This work is concerned with the existence and uniqueness of boundary value problems defined on semi-infinite intervals. These kinds of problems seldom admit exactly known solutions and, therefore, the theoretical information on their well-posedness is essential before attempting to derive an approximate solution by analytical or numerical means. Our utmost contribution in this context is the definition of a numerical test for investigating the existence and uniqueness of solutions of boundary problems defined on semi-infinite intervals. The main result is given by a theorem relating the existence and uniqueness question to the number of real zeros of a function implicitly defined within the formulation of the iterative transformation method. As a consequence, we can investigate the existence and uniqueness of solutions by studying the behaviour of that function. Within such a context, the numerical test is illustrated by two examples where we find meaningful numerical results.
Effect of Quercetin on Mycorrhizal Synthesis between Tuberborchii and Arbutusunedo L. In Vitro Plants
Authors: Gomes Bárbara , Castro Fábio , Santos Rita , Figueiredo Patrícia , Silva Márcia , Vidal Maria , Ferreira Inês , Nunes João , Machado Helena , Gomes Filomena
Published: February 2021

Arbutus unedo L. is a Mediterranean species used for fruit production; it is tolerant to drought and shows regeneration ability following forest fires. Mycorrhizal plants with Tuber borchii add resilience and value. This study aims to test the effect of quercetin on mycorrhizal synthesis between T. borchii and A. unedo. Two genotypes selected for fruit production and hydric stress tolerance, were micropropagated for mycorrhizal synthesis, accomplished during ex vitro rooting in perlite, using lyophilized spores of T. borchii suspended in culture media with different quercetin levels (0–10 µM). Six months after inoculation, plants were transferred to pots and maintained in nursery. Ten and 12 months after inoculation, roots were morphological examined and molecularly characterized using ITS1-5.8SITS2 rDNA region and specific primers. Results showed that mycorrhizae establishment was dependent on studied factors (genotype, quercetin level, and culture medium) and their interaction (genotype X culture medium). Quercetin levels up to 2.0 µM favored mycorrhizae establishment and plant growth, although levels superior to 4 µM showed a toxic effect. Quercetin showed to be an efficient factor on inducing mycorrhiza thriving independent of the genotype. Morphological observations and molecular analysis confirmed the permanence of the fungus association 10 and 12 months after inoculation.