bluuebunny/crossref_metadata_2025 huggingface dataset:
Electroporation-Based Treatments in Urology
Authors: Kiełbik Aleksander , Szlasa Wojciech , Saczko Jolanta , Kulbacka Julita
Published: August 2020

The observation that an application of a pulsed electric field (PEF) resulted in an increased permeability of the cell membrane has led to the discovery of the phenomenon called electroporation (EP). Depending on the parameters of the electric current and cell features, electroporation can be either reversible or irreversible. The irreversible electroporation (IRE) found its use in urology as a non-thermal ablative method of prostate and renal cancer. As its mechanism is based on the permeabilization of cell membrane phospholipids, IRE (as well as other treatments based on EP) provides selectivity sparing extracellular proteins and matrix. Reversible EP enables the transfer of genes, drugs, and small exogenous proteins. In clinical practice, reversible EP can locally increase the uptake of cytotoxic drugs such as cisplatin and bleomycin. This approach is known as electrochemotherapy (ECT). Few in vivo and in vitro trials of ECT have been performed on urological cancers. EP provides the possibility of transmission of genes across the cell membrane. As the protocols of gene electrotransfer (GET) over the last few years have improved, EP has become a well-known technique for non-viral cell transfection. GET involves DNA transfection directly to the cancer or the host skin and muscle tissue. Among urological cancers, the GET of several plasmids encoding prostate cancer antigens has been investigated in clinical trials. This review brings into discussion the underlying mechanism of EP and an overview of the latest progress and development perspectives of EP-based treatments in urology.
Sigma 1 Receptor is Overexpressed in Hepatocellular Adenoma: Involvement of ERα and HNF1α
Authors: Villemain Laure , Prigent Sylvie , Abou-Lovergne Aurélie , Pelletier Laura , Chiral Magali , Pontoglio Marco , Foufelle Fabienne , Caruso Stefano , Pineau Raphael , Rebouissou Sandra , Chevet Eric , Zucman-Rossi Jessica , Combettes Laurent
Published: August 2020

Sigma receptor 1 (SigR1) is an endoplasmic reticulum resident integral membrane protein whose functions remain unclear. Although the liver shows the highest expression of SigR1, its role in this organ is unknown. SigR1 is overexpressed in many cancers and its expression is correlated to hormonal status in hormone-dependent cancers. To better understand the role of SigR1 in hepatocytes we focused our work on the regulation of its expression in tumoral liver. In this context, hepatocellular adenomas, benign hepatic tumors associated with estrogen intake are of particular interest. The expression of SigR1 mRNA was assessed in hepatocellular adenoma (HCA) patients using qPCR. The impact of estrogen on the expression of SigR1 was studied in vivo (mice) and in vitro (HepG2 and Huh7 cells). The effect of HNF1α on the expression of SigR1 was studied in vivo by comparing wild type mice to HNF1 knockout mice. Estrogen enhanced SigR1 expression through its nuclear receptor ERα. HNF1α mutated HCA (H-HCA) significantly overexpressed SigR1 compared to all other HCA subtypes. HNF1 knockout mice showed an increase in SigR1 expression. Overexpressing SigR1 in cellular models increases proliferation rate and storage of lipid droplets, which phenocopies the H-HCA phenotype. SigR1 is involved in hepatocyte proliferation and steatosis and may play an important role in the control of the H-HCA phenotype.
Renal Cell Tumors: Uncovering the Biomarker Potential of ncRNAs
Authors: Outeiro-Pinho Gonçalo , Barros-Silva Daniela , Correia Margareta P. , Henrique Rui , Jerónimo Carmen
Published: August 2020

Renal cell tumors (RCT) remain as one of the most common and lethal urological tumors worldwide. Discrimination between (1) benign and malignant disease, (2) indolent and aggressive tumors, and (3) patient responsiveness to a specific therapy is of major clinical importance, allowing for a more efficient patient management. Nonetheless, currently available tools provide limited information and novel strategies are needed. Over the years, a putative role of non-coding RNAs (ncRNAs) as disease biomarkers has gained relevance and is now one of the most prolific fields in biological sciences. Herein, we extensively sought the most significant reports on ncRNAs as potential RCTs’ diagnostic, prognostic, predictive, and monitoring biomarkers. We could conclude that ncRNAs, either alone or in combination with currently used clinical and pathological parameters, might represent key elements to improve patient management, potentiating the implementation of precision medicine. Nevertheless, most ncRNA biomarkers require large-scale validation studies, prior to clinical implementation.
Splicing Anomalies in Myeloproliferative Neoplasms: Paving the Way for New Therapeutic Venues
Authors: Hautin Marie , Mornet Clélia , Chauveau Aurélie , Bernard Delphine , Corcos Laurent , Lippert Eric
Published: August 2020

Since the discovery of spliceosome mutations in myeloid malignancies, abnormal pre-mRNA splicing, which has been well studied in various cancers, has attracted novel interest in hematology. However, despite the common occurrence of spliceosome mutations in myelo-proliferative neoplasms (MPN), not much is known regarding the characterization and mechanisms of splicing anomalies in MPN. In this article, we review the current scientific literature regarding “splicing and myeloproliferative neoplasms”. We first analyse the clinical series reporting spliceosome mutations in MPN and their clinical correlates. We then present the current knowledge about molecular mechanisms by which these mutations participate in the pathogenesis of MPN or other myeloid malignancies. Beside spliceosome mutations, splicing anomalies have been described in myeloproliferative neoplasms, as well as in acute myeloid leukemias, a dreadful complication of these chronic diseases. Based on splicing anomalies reported in chronic myelogenous leukemia as well as in acute leukemia, and the mechanisms presiding splicing deregulation, we propose that abnormal splicing plays a major role in the evolution of myeloproliferative neoplasms and may be the target of specific therapeutic strategies.
Genetic and Epigenetic Regulation of the Smoothened Gene (SMO) in Cancer Cells
Authors: Lou Hong , Li Hongchuan , Huehn Andrew R. , Tarasova Nadya I. , Saleh Bahara , Anderson Stephen K. , Dean Michael
Published: August 2020

(1) Background: The hedgehog (HH) signaling pathway is a key regulator of embryonic patterning, tissue regeneration, stem cell renewal, and cancer growth. The smoothened (SMO) protein regulates the HH signaling pathway and has demonstrated oncogenic activity. (2) Methods: To clarify the role of the HH signaling pathway in tumorigenesis, the expression profile of key HH signaling molecules, including SMO, PTCH1, GLI1, GLI2, and GLI3, were determined in 33 cancer cell lines and normal prostate cells and tissues. We performed a computational analysis of the upstream region of the SMO gene to identify the regulatory elements. (3) Results: Three potential CpG islands and several putative SMO promoter elements were identified. Luciferase reporter assays mapped key SMO promoter elements, and functional binding sites for SP1, AP1, CREB, and AP-2α transcription factors in the core SMO promoter region were confirmed. A hypermethylated SMO promoter was identified in several cancer cell lines suggesting an important role for epigenetic silencing of SMO expression in certain cancer cells. (4) Discussion: These results have important implications for our understanding of regulatory mechanisms controlling HH pathway activity and the molecular basis of SMO gene function. Moreover, this study may prove valuable for future research aimed at producing therapeutic downregulation of SMO expression in cancer cells.
Understanding the Role of Innate Immune Cells and Identifying Genes in Breast Cancer Microenvironment
Authors: Shihab Israa , Khalil Bariaa A. , Elemam Noha Mousaad , Hachim Ibrahim Y. , Hachim Mahmood Yaseen , Hamoudi Rifat A. , Maghazachi Azzam A.
Published: August 2020

The innate immune system is the first line of defense against invading pathogens and has a major role in clearing transformed cells, besides its essential role in activating the adaptive immune system. Macrophages, dendritic cells, NK cells, and granulocytes are part of the innate immune system that accumulate in the tumor microenvironment such as breast cancer. These cells induce inflammation in situ by secreting cytokines and chemokines that promote tumor growth and progression, in addition to orchestrating the activities of other immune cells. In breast cancer microenvironment, innate immune cells are skewed towards immunosuppression that may lead to tumor evasion. However, the mechanisms by which immune cells could interact with breast cancer cells are complex and not fully understood. Therefore, the importance of the mammary tumor microenvironment in the development, growth, and progression of cancer is widely recognized. With the advances of using bioinformatics and analyzing data from gene banks, several genes involved in NK cells of breast cancer individuals have been identified. In this review, we discuss the activities of certain genes involved in the cross-talk among NK cells and breast cancer. Consequently, altering tumor immune microenvironment can make breast tumors more responsive to immunotherapy.
Pre-Existing Tumoral B Cell Infiltration and Impaired Genome Maintenance Correlate with Response to Chemoradiotherapy in Locally Advanced Rectal Cancer
Authors: Sendoya Juan M. , Iseas Soledad , Coraglio Mariana , Golubicki Mariano , Robbio Juan , Salanova Ruben , Kujaruk Mirta , Mikolaitis Vanesa , Rizzolo Mariana , Ruiz Gonzalo , Cabanne Ana , Gualdrini Ubaldo , Mendez Guillermo , Hirmas Stella , Rotondaro Cecilia , Viglino Julieta , Eleta Martín , Fernandez Elmer , Abba Martín , Podhajcer Osvaldo , Roca Enrique , Llera Andrea S
Published: August 2020

Locally advanced rectal cancer (LARC) remains a medical challenge. Reliable biomarkers to predict which patients will significantly respond to neoadjuvant chemoradiotherapy (nCRT) have not been identified. We evaluated baseline genomic and transcriptomic features to detect differences that may help predict response to nCRT. Eligible LARC patients received nCRT (3D-LCRT 50.4 Gy plus capecitabine 825 mg/m2/bid), preceded by three cycles of CAPOX in high systemic-relapse risk tumors, and subsequent surgery. Frozen tumor biopsies at diagnosis were sequenced using a colorectal cancer panel. Transcriptomic data was used for pathway and cell deconvolution inferential algorithms, coupled with immunohistochemical validation. Clinical and molecular data were analyzed according to nCRT outcome. Pathways related to DNA repair and proliferation (p < 0.005), and co-occurrence of RAS and TP53 mutations (p = 0.001) were associated with poor response. Enrichment of expression signatures related to enhanced immune response, particularly B cells and interferon signaling (p < 0.005), was detected in good responders. Immunohistochemical analysis of CD20+ cells validated the association of good response with B cell infiltration (p = 0.047). Findings indicate that the presence of B cells is associated with successful tumor regression following nCRT in LARC. The prevalence of simultaneous RAS and TP53 mutations along with a proficient DNA repair system that may counteract chemoradio-induced DNA damage was associated with poor response.
Immobilization of Arabidopsis thaliana Hydroxynitrile Lyase (AtHNL) on EziG Opal
Authors: Coloma José , Lugtenburg Tim , Afendi Muhammad , Lazzarotto Mattia , Bracco Paula , Hagedoorn Peter-Leon , Gardossi Lucia , Hanefeld Ulf
Published: August 2020

Arabidopsis thaliana hydroxynitrile lyase (AtHNL) catalyzes the selective synthesis of (R)-cyanohydrins. This enzyme is unstable under acidic conditions, therefore its immobilization is necessary for the synthesis of enantiopure cyanohydrins. EziG Opal is a controlled porosity glass material for the immobilization of His-tagged enzymes. The immobilization of His6-tagged AtHNL on EziG Opal was optimized for higher enzyme stability and tested for the synthesis of (R)-mandelonitrile in batch and continuous flow systems. AtHNL-EziG Opal achieved 95% of conversion after 30 min of reaction time in batch and it was recycled up to eight times with a final conversion of 80% and excellent enantioselectivity. The EziG Opal carrier catalyzed the racemic background reaction; however, the high enantioselectivity observed in the recycling study demonstrated that this was efficiently suppressed by using citrate/phosphate buffer saturated methyl-tert-butylether (MTBE) pH 5 as reaction medium. The continuous flow system achieved 96% of conversion and excellent enantioselectivity at 0.1 mL min−1. Lower conversion and enantioselectivity were observed at higher flow rates. The specific rate of AtHNL-EziG Opal in flow was 0.26 mol h−1 genzyme−1 at 0.1 mL min−1 and 96% of conversion whereas in batch, the immobilized enzyme displayed a specific rate of 0.51 mol h−1 genzyme−1 after 30 min of reaction time at a similar level of conversion. However, in terms of productivity the continuous flow system proved to be almost four times more productive than the batch approach, displaying a space-time-yield (STY) of 690 molproduct h−1 L−1 genzyme−1 compared to 187 molproduct h−1 L−1 genzyme−1 achieved with the batch system.
Ordered Mesoporous Carbon as a Support of Pd Catalysts for CO2 Electrochemical Reduction
Authors: Pérez-Rodríguez Sara , Pastor Elena , Lázaro María Jesús
Published: August 2020

Ordered mesoporous carbons (OMCs) have been used as catalyst supports of Pd nanoparticles for the electrochemical reduction of CO2 in 0.1 M KHCO3. OMC with tunable porosity and morphology were obtained by the hard-template approach using synthesized SBA-15 templates. SBA-15 materials were prepared using a mass ratio of the silica precursor (TEOS) and the surfactant (P123) of 2 or 5. After removing silica particles by a purification treatment with NaOH-ethanol or HF, different OMCs were obtained, with a developed porosity (up to 1050 m2 g−1) and ordered 2D hexagonal mesostructure (p6 mm). An increase in the TEOS/P123 ratio as well as the treatment with HF resulted in a decrease in the structural ordering of the materials. Pd nanoparticles with an average size of around 3 nm were deposited on the OMC. However, larger nanoparticles were also observed, especially for the materials obtained using a mass ratio TEOS/P123 of 5. Despite these differences, electrochemical experiments showed that CO2 was successfully reduced to other species (mainly COad) for all Pd/OMC electrocatalysts. These reduced species were adsorbed on Pd active sites, inhibiting the hydrogen evolution reaction.
SI: Air Pollution and Plant Ecosystems
Authors: Agathokleous Evgenios , Carrari Elisa , Sicard Pierre
Published: August 2020

Air pollution continues to be a serious issue for plant health and terrestrial ecosystems. In this issue of climate, some papers relevant to air pollution and its potential impacts on plant health and terrestrial ecosystems are collated. The papers provide some new insights and offer the opportunity to further advance the current understandings of air pollution and its linked impacts at different levels.
Possibilities of Electromagnetic Penetration of Displays of Multifunction Devices
Authors: Kubiak Ireneusz , Przybysz Artur , Musial Slawomir
Published: August 2020

A protection of information against electromagnetic penetration is very often considered in the aspect of the possibility of obtaining data contained in printed documents or displayed on screen monitors. However, many printing devices are equipped with screens based on LED technology or liquid crystal displays. Options enabling the selection of parameters of the printed document, technical settings of the device (e.g., screen activity time) are the most frequently displayed information. For more extensive displays, more detailed information appears, which may contain data that are not always irrelevant to third parties. Such data can be: names of printed documents (or documents registered and available on the internal media), service password access, user names or regular printer user activity. The printer display can be treated as a source of revealing emissions, like a typical screen monitor. The emissions correlated with the displayed data may allow us to obtain the abovementioned information. The article includes analyses of various types of computer printer displays. The tests results of the existing threat are presented in the form of reconstructed images that show the possibility of reading the text data contained in them.
Addressing Bandwidth-Driven Flow Allocationin RINA
Authors: Koutenský Michal , Veselý Vladimír , Maffione Vincenzo
Published: August 2020

Effective capacity allocation is essential for a network to operate properly, providing predictable quality of service guarantees and avoiding bottlenecks. Achieving capacity allocation fairness is a long-standing problem extensively researched in the frame of transport and network layer protocols such as TCP/IP. The Recursive InterNetwork Architecture offers programmable policies that enable more flexible control on the mechanics of network flow allocation. In this paper, we present our version of one of these policies, which provides flow allocation according to the bandwidth requirements of requesting applications. We implement the bandwidth-aware flow allocation policy by extending rlite, an open source RINA implementation. Our evaluation shows how the policy can prevent links from becoming oversaturated and use alternate paths to achieve high total link data-rate use.
Examining the Influence of Cosmetics on Jury Decisions
Authors: Batres Carlota , Russell Richard
Published: August 2020

Many studies have examined how defendant characteristics influence jury decisions, but none have investigated the effect of cosmetics. We therefore examined how cosmetics influence jury decisions for young and middle-aged female defendants. In Study 1, participants were more likely to assign guilty verdicts to middle-aged defendants than young defendants and when presented with cosmetics, male participants gave young defendants longer sentences and middle-aged defendants shorter sentences. In Study 2, however, we did not replicate the age or the cosmetics effects on jury sentences, suggesting that comparisons between defendants may have influenced jury decisions in Study 1. Further work is thus still needed, but our two well-powered studies (N = 1127) provide a first exploration into the influence of cosmetics on jury decisions.
An Uncontrolled Case Series Using a Botanically Derived, β-Cyclodextrin Inclusion Complex in Two Androgenetic Alopecia-Affected Male Subjects
Authors: Marcovici Geno , Bauman Alan
Published: August 2020

Drug-based monotherapy provides limited clinical benefits in polygenic disorders, such as androgenetic alopecia. Possible benefits must be measured against non-trivial risks of negative side effects. Several well-controlled, peer-reviewed, basic science studies have demonstrated novel mechanisms of action and potential utility for natural-based phytochemicals in the treatment of androgen-mediated disorders, including androgenetic alopecia. Yet, due to phytochemical instability, volatility, and incompatibility, the bridge from in vitro potential to clinical efficacy remains largely unmet. Recent advances in nanomaterial manipulation provide enhanced platforms, such as cyclodextrins, in which these phytochemicals may be enveloped and delivered without triggering the loss of intended function. Unexpected, positive results of an uncontrolled case series for a cyclodextrin-enabled, natural-based formula containing γ linolenic acid, β-Sitosterol, epigallocatechin gallate, and genistein, administered concomitantly via oral and topical form in two androgenetic alopecia-affected, male subjects over the course of 270 days were found. At baseline, significant baldness in the vertex scalp of both subjects was observed. Subsequent 90-day time points demonstrated marked hair thickening. On treatment day 270 (conclusion), scalp hair loss was no longer evident in either patient. Particularly in the setting of a disorders, such as androgenetic alopecia, nano-complexed, botanically-based compositions may offer beneficial adjunctives or alternatives to traditional drug-based/surgical medical treatments.
Spatial and Temporal Trends of Burnt Area in Angola: Implications for Natural Vegetation and Protected Area Management
Authors: Catarino Silvia , Romeiras Maria Manuel , Figueira Rui , Aubard Valentine , Silva João M. N. , Pereira José M. C.
Published: August 2020

Fire is a key driver of natural ecosystems in Africa. However, human activity and climate change have altered fire frequency and severity, with negative consequences for biodiversity conservation. Angola ranks among the countries with the highest fire activity in sub-Saharan Africa. In this study, we investigated the spatial and temporal trends of the annual burnt area in Angola, from 2001 to 2019, and their association with terrestrial ecoregions, land cover, and protected areas. Based on satellite imagery, we analyzed the presence of significant trends in burnt area, applying the contextual Mann–Kendall test and the Theil–Sen slope estimator. Data on burnt areas were obtained from the moderate-resolution imaging spectroradiometer (MODIS) burnt area product and the analyses were processed in TerrSet. Our results showed that ca. 30% of the country’s area burned every year. The highest percentage of annual burnt area was found in northeast and southeast Angola, which showed large clusters of decreasing trends of burnt area. The clusters of increasing trends were found mainly in central Angola, associated with savannas and grasslands of Angolan Miombo woodlands. The protected areas of Cameia, Luengue-Luiana, and Mavinga exhibited large areas of decreasing trends of burnt area. Conversely, 23% of the Bicuar National Park was included in clusters of increasing trends. Distinct patterns of land cover were found in areas of significant trends, where the clusters of increasing trends showed a higher fraction of forest cover (80%) than the clusters of decreasing trends (55%). The documentation of burnt area trends was very important in tropical regions, since it helped define conservation priorities and management strategies, allowing more effective management of forests and fires in countries with few human and financial resources.
Displacements of an Active Moderately Rapid Landslide—A Dataset Retrieved by Continuous GNSS Arrays
Authors: Mulas Marco , Ciccarese Giuseppe , Truffelli Giovanni , Corsini Alessandro
Published: August 2020

This paper describes a dataset of continuous GNSS positioning solutions referring to slope movements in the Ca’ Lita landslide (Northern Apennines, Italy). The dataset covers the period from 24 March 2016 to 17 July 2019 and includes time-series of the daily position of three GNSS rovers located in different parts of the landslide: head zone, upper track zone, and lower track zone. Two different types of continuous GNSS arrays have been used: one is based on high-end Leica geodetic receivers, and the other is based on low-cost effective Emlid receivers. Displacements captured in the dataset are up to more than a hundred meters and are characterized by prolonged phases of slow movement and moderately rapid acceleration phases. The data presented in this contribution were used to underline slope processes and validate displacements retrieved by the application of digital image correlation to a stack of a satellite images.
Cystic Fibrosis-Screening Positive Inconclusive Diagnosis: Newborn Screening and Long-Term Follow-Up Permits to Early Identify Patients with CFTR-Related Disorders
Authors: Castaldo Alice , Cimbalo Chiara , Castaldo Raimondo J. , D’Antonio Marcella , Scorza Manuela , Salvadori Laura , Sepe Angela , Raia Valeria , Tosco Antonella
Published: August 2020

Background: Newborn screening (NBS) early-identifies cystic fibrosis (CF), but in CF-screening positive inconclusive diagnosis (CF-SPID) the results of immunoreactive trypsinogen (IRT), molecular analysis and sweat test (ST) are discordant. A percentage of CF-SPID evolves to CF, but data on long-term monitoring are lacking. We describe the follow-up of all CF and CF-SPID identified between 2008 and 2019. Methods: NBS was performed by IRT followed by molecular analysis and ST between 2008 and 2014; double IRT followed by molecular analysis and ST after 2014. Results: NBS revealed 47 CF and 99 CF-SPID newborn, a ratio 1:2.1—the highest reported so far. This depends on the identification by gene sequencing of the second variant with undefined effect in 40 CF-SPID that otherwise would have been defined as carriers. Clinical complications and pulmonary infections occurred more frequently among CF patients than among CF-SPID. Two CF-SPID cases evolved to CF (at two years), while eight evolved to CFTR-related disorders (CFTR-RD), between one and eight years, with bronchiectasis (two), recurrent pneumonia (four, two with sinonasal complications), recurrent pancreatitis (two). No clinical, biochemical or imaging data predicted the evolution. Conclusion: Gene sequencing within the NBS reveals a higher number of CF-SPID and we first describe an approach to early identify CFTR-RD, with relevant impact on their outcome.
Screening Strategy of Pancreatic Cancer in Patients with Diabetes Mellitus
Authors: Mizuno Suguru , Nakai Yousuke , Ishigaki Kazunaga , Saito Kei , Oyama Hiroki , Hamada Tsuyoshi , Suzuki Yukari , Inokuma Akiyuki , Kanai Sachiko , Noguchi Kensaku , Sato Tatsuya , Hakuta Ryunosuke , Saito Tomotaka , Takahara Naminatsu , Kogure Hirofumi , Isayama Hiroyuki , Koike Kazuhiko
Published: August 2020

The incidence of pancreatic cancer (PCa) is increasing worldwide and has become one of the leading causes of cancer-related death. Screening for high risk populations is fundamental to overcome this intractable malignancy. Diabetes mellitus (DM) is classically known as a risk factor for PCa. Recently the reverse causality is in the spotlight, that is to say, DM is considered to be a manifestation of PCa. Numbers of epidemiological studies clarified that new-onset DM (≤2-year duration) was predominant in PCa patients and the relative risk for PCa inversely correlated with duration of DM. Among patients with new-onset DM, elder onset, weight loss, and rapid exacerbation of glycemic control were reported to be promising risk factors and signs, and the model was developed by combining these factors. Several pilot studies disclosed the possible utility of biomarkers to discriminate PCa-associated DM from type 2 DM. However, there is no reliable biomarkers to be used in the practice. We previously reported the application of a multivariate index for PCa based on the profile of plasma free amino acids (PFAAs) among diabetic patients. We are further investigating on the PFAA profile of PCa-associated DM, and it can be useful for developing the novel biomarker in the near future.
Low Myostatin Serum Levels Are Associated with Poor Outcome in Critically Ill Patients
Authors: Wirtz Theresa H. , Loosen Sven H. , Buendgens Lukas , Kurt Berkan , Abu Jhaisha Samira , Hohlstein Philipp , Brozat Jonathan F. , Weiskirchen Ralf , Luedde Tom , Tacke Frank , Trautwein Christian , Roderburg Christoph , Koch Alexander
Published: August 2020

Background: Growth differentiation factor 8, GDF-8 (Myostatin), is a protein released by myocytes inhibiting muscle growth and differentiation. Serum concentrations of Myostatin can predict poor survival in different chronic diseases, but its role in critical illness and sepsis is obscure. Our aim was to investigate Myostatin levels as a potential prognostic biomarker in critically ill patients with sepsis. Methods: We therefore measured Myostatin serum concentrations in 165 critically ill patients (106 with sepsis, 59 without sepsis) upon admission to the medical intensive care unit (ICU), in comparison to 14 healthy controls. Results: Myostatin levels were significantly decreased in ICU patients compared to controls but did not differ in patients with or without sepsis. However, Myostatin concentrations were significantly lower in patients requiring mechanical ventilation and indicated a trend towards dependency of intravenous vasopressors. Interestingly, we observed a negative correlation between Myostatin levels and markers of systemic inflammation. Strikingly, overall survival (OS) was significantly impaired in patients with low Myostatin levels in all critically ill patients. Low Myostatin levels at baseline turned out as an independent prognostic marker for OS in multivariate Cox-regression analysis (HR: 0.433, 95% CI: 0.211–0.889, p = 0.023). Conclusions: In summary, serum Myostatin concentrations are significantly decreased in critically ill patients and associated with disease severity. Low Myostatin levels also identify a subgroup of ICU patients that are more likely to face an unfavorable clinical outcome in terms of OS.
Preliminary Post-Mortem COVID-19 Evidence of Endothelial Injury and Factor VIII Hyperexpression
Authors: Cipolloni Luigi , Sessa Francesco , Bertozzi Giuseppe , Baldari Benedetta , Cantatore Santina , Testi Roberto , D’Errico Stefano , Di Mizio Giulio , Asmundo Alessio , Castorina Sergio , Salerno Monica , Pomara Cristoforo
Published: August 2020

(1) Background: The current outbreak of COVID-19 infection is an ongoing challenge and a major threat to public health that requires surveillance, prompt diagnosis, as well as research efforts to understand the viral pathogenesis. Despite this, to date, very few studies have been performed concerning autoptic specimens. Therefore, this study aimed: (i) to reiterate the importance of the autoptic examination, the only method able to precisely define the cause of death; (ii) to provide a complete post-mortem histological and immunohistochemical investigation pattern capable of diagnosing death from COVID-19 infection. (2) Methods: In this paper, the lung examination of two subjects who died from COVID-19 are discussed, comparing the obtained data with those of the control, a newborn who died from pneumonia in the same pandemic period. (3) Results: The results of the present study suggest that COVID-19 infection can cause different forms of acute respiratory distress syndrome (ARDS), due to diffuse alveolar damage and diffuse endothelial damage. Nevertheless, different patterns of cellular and cytokine expression are associated with anti-COVID-19 antibody positivity, compared to the control case. Moreover, in both case studies, it is interesting to note that COVID-19, ACE2 and FVIII positivity was detected in the same fields. (4) Conclusions: COVID-19 infection has been initially classified as exclusively interstitial pneumonia with varying degrees of severity. Subsequently, vascular biomarkers showed that it can also be considered a vascular disease. The data on Factor VIII discussed in this paper, although preliminary and limited in number, seem to suggest that the thrombogenicity of Sars-CoV2 infection might be linked to widespread endothelial damage. In this way, it would be very important to investigate the pro-coagulative substrate both in all subjects who died and in COVID-19 survivors. This is because it may be hypothesized that the different patterns with which the pathology is expressed could depend on different individual susceptibility to infection or a different personal genetic-clinical background. In light of these findings, it would be important to perform more post-mortem investigations in order to clarify all aspects of the vascular hypothesis in the COVID-19 infection.
Evaluation of MicroRNAs as Non-Invasive Diagnostic Markers in Urinary Cells from Patients with Suspected Prostate Cancer
Authors: Borkowetz Angelika , Lohse-Fischer Andrea , Scholze Jana , Lotzkat Ulrike , Thomas Christian , Wirth Manfred P. , Fuessel Susanne , Erdmann Kati
Published: August 2020

Currently used tumor markers for early diagnosis of prostate cancer (PCa) are often lacking sufficient specificity and sensitivity. Therefore, the diagnostic potential of selected microRNAs in comparison to serum PSA levels and PSA density (PSAD) was explored. A panel of 12 PCa-associated microRNAs was quantified by qPCR in urinary sediments from 50 patients with suspected PCa undergoing prostate biopsy, whereupon PCa was detected in 26 patients. Receiver operating characteristic (ROC) curve analyses revealed a potential for non-invasive urine-based PCa detection for miR-16 (AUC = 0.744, p = 0.012; accuracy = 76%) and miR-195 (AUC = 0.729, p = 0.017; accuracy = 70%). While serum PSA showed an insufficient diagnostic value (AUC = 0.564, p = 0.656; accuracy = 50%) in the present cohort, PSAD displayed an adequate diagnostic performance (AUC = 0.708, p = 0.031; accuracy = 70%). Noteworthy, the combination of PSAD with the best candidates miR-16 and miR-195 either individually or simultaneously improved the diagnostic power (AUC = 0.801–0.849, p < 0.05; accuracy = 76–90%). In the sub-group of patients with PSA ≤ 10 ng/mL (n = 34), an inadequate diagnostic power of PSAD alone (AUC = 0.595, p = 0.524; accuracy = 68%) was markedly surpassed by miR-16 and miR-195 individually as well as by their combination with PSAD (AUC = 0.772–0.882, p < 0.05; accuracy = 74–85%). These findings further highlight the potential of urinary microRNAs as molecular markers with high clinical performance. Overall, these results need to be validated in a larger patient cohort.
Tobacco Products, Periodontal Health and Education Level: Cohort Study from Sweden
Authors: Julkunen-Iivari Anna , Heikkinen Anna Maria , Räisänen Ismo T. , Ruokonen Hellevi , Meurman Jukka H. , Toppila-Salmi Sanna , Söder Per-Östen , Söder Birgitta
Published: August 2020

Background: The aim of this study is to investigate if using tobacco products (including snuff, smoking tobacco and dual-using) associates with periodontal health, education level and mortality in a Swedish cohort, hypothesizing that tobacco products affect periodontal health, associate with lower education and increase the risk of death. Method: Study cohort of 1080 subjects aged 31–40 years (528 men, 552 women) was clinically examined and interviewed in 1985 and followed for mortality until 2015. Subjects were classified into two groups: “tobacco users” and “non-users”. Associations between periodontal health parameters, tobacco products, education level and age of death were analysed. SPSS was used for analyses. Results: Tobacco products, as well as education level associated, with poor periodontal health. Tobacco users and lower education was linked to higher plaque-, calculus- and gingival-index scores than non-users (p < 0.001). They also had significantly higher prevalence of deep periodontal pockets (≥5 mm) (p < 0.001 and 0.010, respectively), missing teeth (p = 0.010 and 0.003, respectively) and lower education level (p < 0.001) compared with non-users. However, tobacco product users did not die significantly earlier than non-users. Conclusion: Tobacco products had a negative impact on periodontal health. Tobacco product users were less educated. However, using tobacco products may not cause premature death.
Optimization of Probabilistic Shaping for Nonlinear Fiber Channels with Non-Gaussian Noise
Authors: Hansen Henrik Enggaard , Yankov Metodi P. , Oxenløwe Leif Katsuo , Forchhammer Søren
Published: August 2020

Probabilistic constellation shaping is investigated in the context of nonlinear fiber optic communication channels. Based on a general framework, different link types are considered—1. dispersion-managed channels, 2. unrepeatered transmission channels and 3. ideal distributed Raman amplified channels. These channels exhibit nonlinear effects to a degree that conventional probabilistic constellation shaping strategies for the additive white Gaussian (AWGN) noise channel are suboptimal. A channel-agnostic optimization strategy is used to optimize the constellation probability mass functions (PMFs) for the channels in use. Optimized PMFs are obtained, which balance the effects of additive amplified spontaneous emission noise and nonlinear interference. The obtained PMFs cannot be modeled by the conventional Maxwell-Boltzmann PMFs and outperform optimal choices of these in all the investigated channels. Suboptimal choices of constellation shapes are associated with increased nonlinear effects in the form of non-Gaussian noise. For dispersion-managed channels, a reach gain in 2 spans is seen and across the three channel types, gains of >0.1 bits/symbol over unshaped quadrature-amplitude modulation (QAM) are seen using channel-optimized probablistic shaping.
Conditional Lie-Bäcklund Symmetries and Differential Constraints of Radially Symmetric Nonlinear Convection-Diffusion Equations with Source
Authors: Ji Lina , Wang Rui
Published: August 2020

A conditional Lie-Bäcklund symmetry method and differential constraint method are developed to study the radially symmetric nonlinear convection-diffusion equations with source. The equations and the admitted conditional Lie-Bäcklund symmetries (differential constraints) are identified. As a consequence, symmetry reductions to two-dimensional dynamical systems of the resulting equations are derived due to the compatibility of the original equation and the additional differential constraint corresponding to the invariant surface equation of the admitted conditional Lie-Bäcklund symmetry.
Towards a More Realistic Citation Model: The Key Role of Research Team Sizes
Authors: Milojević Staša
Published: August 2020

We propose a new citation model which builds on the existing models that explicitly or implicitly include “direct” and “indirect” (learning about a cited paper’s existence from references in another paper) citation mechanisms. Our model departs from the usual, unrealistic assumption of uniform probability of direct citation, in which initial differences in citation arise purely randomly. Instead, we demonstrate that a two-mechanism model in which the probability of direct citation is proportional to the number of authors on a paper (team size) is able to reproduce the empirical citation distributions of articles published in the field of astronomy remarkably well, and at different points in time. Interpretation of our model is that the intrinsic citation capacity, and hence the initial visibility of a paper, will be enhanced when more people are intimately familiar with some work, favoring papers from larger teams. While the intrinsic citation capacity cannot depend only on the team size, our model demonstrates that it must be to some degree correlated with it, and distributed in a similar way, i.e., having a power-law tail. Consequently, our team-size model qualitatively explains the existence of a correlation between the number of citations and the number of authors on a paper.
A Mobile-Based Tailored Recommendation System for Parents of Children with Overweight or Obesity: A New Tool for Health Care Centers
Authors: Afonso Lisa , Rodrigues Rui , Castro Joana , Parente Nuno , Teixeira Carina , Fraga Ana , Torres Sandra
Published: August 2020

Childhood obesity is associated with unbalanced lifestyle patterns, and new strategies are needed to support parents in the compliance with the guidelines for children’s age. Tailored automatic recommendations mimic interpersonal counseling and are promising strategies to be considered for health promotion programs. This study aimed to develop and test a mobile recommendation system for parents of preschool children identified with overweight/obesity at health care centers. Evidence-based recommendations related to children’s eating, drinking, moving, and sleeping habits were developed and tested using a questionnaire. A pilot study was conducted in a health care center to test how using an app with those tailored recommendations, in video format, influenced parents’ perceptions of the child’s weight status and their knowledge about the guidelines, compared to a control group. The chi-squared test was used for categorical variables and the Mann–Whitney U test for continuous variables (p < 0.05). A high proportion of parents were already informed about the guidelines, but their children were not meeting them. After watching the tailored recommendations, there was an increased knowledge of the guideline on water intake, but there was no improvement in the perception of the child’s excessive weight. Parents may benefit from a mobile-based tailored recommendation system to improve their knowledge about the guidelines. However, there is a need to work with parents on motivation to manage the child’s weight with additional strategies.
Experimental Comparisons and Evaluations of Different Types of DC-link Capacitors for VSI-Based Electric Compressors in Battery Electric Vehicle Systems
Authors: Kim Namhun , Park Changju , Kwak Sangshin , Baek Jeihoon
Published: August 2020

Electric compressor systems for air conditioning operations are an essential part in battery electric vehicle systems, which are not applicable to conventional belt-driven compressors due to no combustion engines. Three-phase voltage source inverters (VSI) and interior permanent magnet (IPM) motors are generally used for electric compressor systems in battery electric vehicles. Direct current (DC)-link capacitors are a critical component in the power converter systems, which affect the cost, size, performances and scale. Metallized polypropylene film capacitors are considered more reliable than conventional electrolytic capacitors for high temperature environments such as electric vehicle applications. This paper presents comprehensive comparisons and evaluations of electric compressors with two types of DC-link capacitors. Based on a 5 kW IPM motor drives and a VSI with a nominal DC voltage of 360 V for electric compressors, performances with electrolytic and film capacitors have been evaluated by experimental tests.
A Novel Image Encryption Scheme Based on 2D Fractional Chaotic Map, DWT and 4D Hyper-chaos
Authors: Ding Lina , Ding Qun
Published: August 2020

In this paper, a novel image encryption scheme based on a fractional-order Henon chaotic map, a two-dimensional (2D) Discrete Wavelet Transform (DWT) and a four-dimensional (4D) hyperchaotic system is proposed. Firstly, the original image is transformed and scrambled by the 2D DWT, and then the image is shuffled with the fractional-order Henon chaotic time series. Finally, the shuffled image is diffused and encrypted by the 4D hyperchaos system. Through the application of DWT and high-low dimensional chaotic systems, the encryption effect of this algorithm is better than those done by single or ordinary chaotic encryption algorithm, and it has a larger key space and higher security. The experimental tests show that the system has good statistical characteristics, such as histogram analysis, correlation coefficient analysis, key space and key sensitivity, information entropy analysis and so on. The encryption algorithm also passes the relevant security attack tests with good security.
The Zr-Doped CaO CO2 Sorbent Fabricated by Wet High-Energy Milling
Authors: Rodaev Vyacheslav V. , Razlivalova Svetlana S.
Published: August 2020

We fabricated the Zr-doped CaO sorbent for high-temperature CO2 capture by the wet high-energy co-milling of calcium carbonate and natural zirconium dioxide (baddeleyite) for the first time. The morphology of the material was examined by scanning electron microscopy, energy-dispersive X-ray analysis and X-ray diffraction. Its CO2 uptake capacity was determined using thermogravimetric analysis. After 50 carbonation–calcination cycles, the Zr-doped CaO sorbent characterized by a high enough CO2 uptake capacity of 8.6 mmol/g and unchanged microstructure due to CaZrO3 nanoparticles uniformly distributed in the CaO matrix to prevent CaCO3 sintering under carbonation. The proposed easy-to-implement CaO-based sorbents fabrication technique is promising for industrial application.
An Integrated Three-Level Synergetic and Reliable Optimization Method Considering Heat Transfer Process, Component, and System
Authors: Zhao Tian , Liu Di , He Ke-Lun , Chen Xi , Chen Qun
Published: August 2020

Optimization of heat transfer systems (HTSs) benefits energy efficiency. However, current optimization studies mainly focus on the improvement of system design, component design, and local process intensification separately, which may miss the optimal results and lack reliability. This work proposes a synergetic optimization method integrating levels of the local process, component to system, which could guarantee the reliability of results. The system-level optimization employs the heat current method and hydraulic analysis, the component level optimization adopts heuristic optimization algorithm, and the process level optimization applies the field synergy principle. The introduction of numerical simulation and iteration provides the self-consistency and credibility of results. Optimization results of a multi-loop heat transfer system present that the proposed method can save 16.3% pumping power consumption comparing to results only considering system and process level optimization. Moreover, the optimal parameters of component originate from the trade-off relation between two competing mechanisms of performance enhancement, i.e., the mass flow rate increase and shape variation. Finally, the proposed method is not limited to heat transfer systems but also applicable to other thermal systems.
Perspectives for Mitigation of CO2 Emission due to Development of Electromobility in Several Countries
Authors: Tucki Karol , Orynycz Olga , Mitoraj-Wojtanek Mateusz
Published: August 2020

The creep trend method is used for the analysis of the development of electric car production in three regions: The United States, the European Union and Japan. Based on vehicle registration and population growth data for each year the creep trend method using historical data for the years 2007–2017 is applied for forecasting development up to 2030. Moreover, the original method for calculating the primary energy factor (PEF) was applied to the analysis of power engineering systems in the regions investigated. The assessment of the effects of electromobility development on air quality has been performed, reduction values for pollutant and greenhouse gas emissions have been determined, which was the main objective of this manuscript. Mitigation of air pollutant emissions, i.e., carbon dioxide (CO2), carbon monoxide (CO) and nitrogen oxides (NOx) was estimated and compared to the eventual expected increase of emissions from power plants due to an increase of the demand for electricity. It can be concluded that electricity powered cars along with appropriate choices of energetic resources as well as electricity distribution management will play the important role to achieve the sustainable energy economy. Based on the emission reduction projections resulting from the projected increase in the number of electric cars, (corrected) emissions will be avoided in 2030 in the amount of over 14,908,000 thousand tonnes CO2 in European Union, 3,786,000 thousand tonnes CO2 in United States and 111,683 thousand tonnes CO2 in Japan.