bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of sodium butyrate on slaughter performance, serum indexes and intestinal barrier of rabbits
Authors: Li Chenyang , Chen Jiali , Zhao Man , Liu Mengqi , Yue Zhengkai , Liu Lei , Li Fuchang
Published: June 2021

The purpose of this study was to investigate the effect of sodium butyrate on slaughter performance, serum indexes and the intestinal barrier in rabbits. Six hundred healthy weaned rabbits were randomly divided into three groups (5 replicates per group, 40 rabbits per replicate): control (fed a basal diet), sodium butyrate (fed a basal diet containing 0.5% sodium butyrate) and antibiotic (fed a basal diet containing 0.004% antibiotic). The trial lasted 35 days, including 7 days of pretesting and 28 days of formal testing. The results showed that dietary sodium butyrate supplementation increased the full‐bore weight, the half‐bore weight and the half‐bore rate of rabbits. Meanwhile, the content of aspartate aminotransferase (AST) in serum was increased in rabbits fed the sodium butyrate diet. According to the intestinal barrier, after adding sodium butyrate to feed, the tight junction function of the rabbit intestine is enhanced, and the intestinal microbial composition is also improved. To sum up, after sodium butyrate was added to feed instead of antibiotics, slaughter performance was significantly enhanced, serum indexes were improved, and intestinal barrier function was also enhanced. Therefore, sodium butyrate can be added to feed as an additive and can replace antibiotics.
Received dose variability after administration of I‐131 for treatment of hyperthyroidism in cats
Authors: Busser Suzanne , Poirier Valerie J. , Liggins Carolyn , Bray Sharyn
Published: June 2021

Background

Injectable radioactive iodine (I‐131) frequently is used to treat hyperthyroidism in cats. In human medicine, residual activity after injection of radionuclides has been reported, and the actual quantity administered is recorded after administration.

Objective

Our aim was to evaluate actual administered dose variability after administration of preprepared I‐131 single unit doses for the treatment of hyperthyroidism in cats.

Animals

Twenty‐seven cats with hyperthyroidism treated with I‐131 between April 2017 and March 2019.

Methods

Retrospective observational study of cats treated with preprepared single unit I‐131 doses. For each dose, the measured activity before administration and residual activity were recorded. The measured dose and the actual dose administered were compared to the prescribed dose.

Results

Measured activity before administration ranged from 88.4% to 103.3% of the prescribed dose. Mean residual activity was 5.2 ± 3.0 MBq (ranging from 1.5% to 15% of the prescribed dose). The actual dose administered (measured activity − residual activity) ranged from 79.1% to 100.2% of the prescribed dose. Seventeen of 28 (60.7%) of the actual administered doses differed between 10% and 20% of the prescribed dose. One administered dose had a >20% difference compared to the prescribed dose (79.10% of the prescribed dose).

Conclusion and Clinical Importance

Our study identified variability in the residual and actual administered activity of I‐131 as compared to the prescribed dose, which should be taken into consideration when treating cats with (predrawn) I‐131.
Remediation: The best CPD and vice versa
Authors: Bourgeois‐Law Gisèle
Published: June 2021

Bourgeois‐Law juxtaposes continuing professional development and remediation, highlighting how they share underlying principles and would each benefit from closer collaboration between their communities.
Lon protease downregulates phenazine‐1‐carboxamide biosynthesis by degrading the quorum sensing signal synthase PhzI and exhibits negative feedback regulation of Lon itself in Pseudomonas chlororaphis HT66
Authors: Wang Zheng , Huang Xianqing , Jan Malik , Kong Deyu , Wang Wei , Zhang Xuehong
Published: June 2021

Pseudomonas chlororaphis HT66 exhibits strong antagonistic activity against various phytopathogenic fungi due to its main antibiotic phenazine‐1‐carboxamide (PCN). PCN gene cluster consists of phzABCDEFG, phzH, phzI, and phzR operons. phzABCDEFG transcription is activated by the PhzI/R quorum sensing system. Deletion of the lon gene encoding an ATP‐dependent protease resulted in significant enhancement of PCN production in strain HT66. However, the regulatory pathway and mechanism of Lon on PCN biosynthesis remain unknown. Here, lon mutation was shown to significantly improve antimicrobial activity of strain HT66. The N‐acyl‐homoserine lactone synthase PhzI mediates the negative regulation of PCN biosynthesis and phzABCDEFG transcription by Lon. Western blot showed that PhzI protein abundance and stability were significantly enhanced by lon deletion. The in vitro degradation assay suggested that Lon could directly degrade PhzI protein. However, Lon with an amino acid replacement (S674‐A) could not degrade PhzI protein. Lon‐recognized region was located within the first 50 amino acids of PhzI. In addition, Lon formed a new autoregulatory feedback circuit to modulate its own degradation by other potential proteases. In summary, we elucidated the Lon‐regulated pathway mediated by PhzI during PCN biosynthesis and the molecular mechanism underlying the degradation of PhzI by Lon in P. chlororaphis HT66.
Mary Livermore andMy Story of the War: A nurse’s narrative journey
Authors: Choperena Ana
Published: June 2021

Mary Livermore'sMy Story of the Waris a valuable piece of travel writing written from the point of view of a nurse who documented her unexpected personal and professional journey to administer the Sanitary Commission of the United States Union Army and provide nursing care during the American Civil War. Although Livermore's pre‐war background had not been solely limited to the domestic sphere, her wartime experience involved a public negotiation between the traditional domestic realm assigned to women and new nursing professional functions that emerged during the war. In a context in which the general access of women to public writing was rather limited and in which nursing was not a formally regulated professional activity, Livermore's triumphal narrative reflects the increasing connection between progressively professional nursing functions that emerged in the context of war and a new women's rights leadership forged during her autobiographical journey.
TaPHT1;9‐4B and its transcriptional regulator TaMYB4‐7D contribute to phosphate uptake and plant growth in bread wheat
Authors: Wang Pengfei , Li Gezi , Li Guangwei , Yuan Shasha , Wang Chenyang , Xie Yingxin , Guo Tiancai , Kang Guozhang , Wang Daowen
Published: June 2021

Efficient phosphate (Pi) uptake and utilisation are essential for promoting crop yield. However, the underlying molecular mechanism is still poorly understood in complex crop species such as hexaploid wheat. Here we report that TaPHT1;9‐4B and its transcriptional regulator TaMYB4‐7D function in Pi acquisition, translocation and plant growth in bread wheat.

TaPHT1;9‐4B, a high‐affinity Pi transporter highly upregulated in roots by Pi deficiency, was identified using quantitative proteomics. Disruption of TaPHT1;9‐4B function by BSMV‐VIGS or CRISPR editing impaired wheat tolerance to Pi deprivation, whereas transgenic expression of TaPHT1;9‐4B in rice improved Pi uptake and plant growth. Using yeast‐one‐hybrid assay, we isolated TaMYB4‐7D, a R2R3 MYB transcription factor that could activate TaPHT1;9‐4B expression by binding to its promoter. Silencing TaMYB4‐7D decreased TaPHT1;9‐4B expression, Pi uptake and plant growth.

Four promoter haplotypes were identified for TaPHT1;9‐4B, with Hap3 showing significant positive associations with TaPHT1;9‐4B transcript level, growth performance and phosphorus (P) content in wheat plants. A functional marker was therefore developed for tagging Hap3.

Collectively, our data shed new light on the molecular mechanism controlling Pi acquisition and utilisation in bread wheat. TaPHT1;9‐4B and TaMYB4‐7D may aid further research towards the development of P efficient crop cultivars.
An Intraoperative Trajectory‐Determined Strategy of Patient‐Specific Drill Template for C2 Transoral Pedicle Insertion in Incomplete Reduction of Atlantoaxial Dislocation: An In Vitro Study
Authors: Shan Jing , Zhu Mei‐song , Li Lu‐tao , Peng Peng , Dai Min , Lin Li‐jun , Li Jian‐yi
Published: June 2021

Objectives

This study aims to explore a novel intraoperative trajectory‐determined strategy of grouped patient‐specific drill templates (PDTs) for transoral C2 pedicle screw insertion (C2TOPI) for atlantoaxial dislocation (AAD) with incomplete reduction and to evaluate its efficiency and accuracy.

Methods

Ten cadaveric C2 specimens were scanned by computed tomography (CT) and randomly divided into two groups (the PDT and freehand groups). A novel intraoperative trajectory‐determined strategy of grouped PDTs was created for AAD with incomplete reduction. C2TOPI was performed by use of the PDT technique and the fluoroscopy‐guided freehand technique. After surgery, the screw deviations from the centroid of the cross‐section at the midpoint of the pedicle and screw position grades were assessed in both groups.

Results

Compared to the freehand group, the PDT group had a significantly shorter surgery time than the freehand group (47.7 vs 61.9 min, P < 0.001). The absolute deviations from the centroids between the preoperative designs and postoperative measurements on the axial plane of the pedicle were 1.19 ± 0.25 mm in the PDT group and 1.82 ± 0.51 mm in the freehand group. On the sagittal plane of the pedicle, the corresponding values were 1.10 ± 0.33 mm in the PDT group and 1.70 ± 0.49 mm in the freehand group. The absolute deviations of the free‐hand group on both the axial and sagittal planes were higher than that of the freehand group (P < 0.05 and P < 0.05, respectively). For the grade of screw insertion position, nine (90%) were observed in type I and one (10%) in type II in the PDT group, whereas five (50%) were in type I, three (30%) were in type II, and two (20%) in type III in the freehand group. Statistical differences could not be found between the groups in terms of the screw positions (P > 0.05).

Conclusion

The novel intraoperative trajectory‐determined strategy of grouped PDTs can be used as an accurate and feasible method for C2TOPI for AAD with incomplete reduction.
Factors impacting parental and child satisfaction in the perioperative setting
Authors: Mendoza Beverly A. , Fortier Michelle A. , Trinh Lily N. , Schmid Lauren N. , Kain Zeev N.
Published: June 2021

Understanding the different modifiable and non‐modifiable factors and their positive or negative influence on parental and child satisfaction is essential to providing high‐quality perioperative care. The purpose of this review is to focus on the perioperative environment and to report the various modifiable and non‐modifiable factors that are associated with satisfaction. We found that factors such as quality of clinician‐patient communication, clinician attitudes, teamwork, shared decision‐making, and improved perioperative information were associated with increased parent and child satisfaction. Interventions such as preparation programs integrating role‐play, teaching of coping skills, and family‐centered programs were highly rated by parents and children. Healthcare providers and institutions should consider the above variables when treating children and their parents in the perioperative setting.
The size and the age of the metabolically active carbon in tree roots
Authors: Hilman Boaz , Muhr Jan , Helm Juliane , Kuhlmann Iris , Schulze Ernst‐Detlef , Trumbore Susan
Published: June 2021

Little is known about the sources and age of C respired by tree roots. Previous research in stems identified two functional pools of non‐structural carbohydrates (NSC): an “active” pool supplied directly from canopy photo‐assimilates supporting metabolism and a “stored” pool used when fresh C supplies are limited. We compared the C isotope composition of water‐soluble NSC and respired CO2for aspen roots (Populus tremulahybrids) cut off from fresh C supply after stem‐girdling or prolonged incubation of excised roots. We used bomb radiocarbon to estimate the time elapsed since C fixation for respired CO2, water‐soluble NSC and structural α‐cellulose. While freshly excised roots (mostly <2.9 mm in diameter) respired CO2fixed <1 year previously, the age increased to 1.6–2.9 year within a week after root excision. Freshly excised roots from trees girdled ~3 months ago had respiration rates and NSC stocks similar to un‐girdled trees but respired older C (~1.2 year). We estimate that over 3 months NSC in girdled roots must be replaced 5–7 times by reserves remobilized from root‐external sources. Using a mixing model and observed correlations between Δ14C of water‐soluble C and α‐cellulose, we estimate ~30% of C is “active” (~5 mg C g−1).
Establishment of cultured primary acral melanoma cells and animal models for Chinese patients
Authors: Hu Rui , Zhao Shuang , Su Juan , Chen Xiang , Yin Mingzhu
Published: June 2021

Acral melanoma is the major subtype of melanoma in Chinese patients. However, a majority of current studies focused on non‐acral melanoma. Most immortalized melanoma cell lines and primary cells were not from acral melanoma. Besides, there are rarely reports about methods for establishing primary acral melanoma cell cultures and related animal models. Here, we present four new human primary acral melanoma cell lines. To determine the mutational profile of the established primary melanoma cells for future targeted use, we performed exome sequencing. We next examined cell proliferation of the primary acral melanoma cells by colony‐formation assays and CCK8 assay. We also evaluated the proliferative and metastatic potential of XYAM‐4 in vivo. We report a detailed protocol for establishing cultured primary acral melanoma cells for Chinese patients and related animal models. We also summarize the features in our acral melanoma cell lines and the existing acral melanoma cell lines. This will provide an effective research tool for research on drug responses and individualized treatment for Chinese patients and comparative studies of melanomas between western and Chinese populations.
Mixed-Frequency Bayesian Predictive Synthesis for Economic Nowcasting
Authors: McAlinn Kenichiro
Published: June 2021

We develop a novel framework for dynamic modelling of mixed-frequency data using Bayesian predictive synthesis. The proposed framework—unlike other mixed-frequency methods—considers data reported at different frequencies as latent factors, in the form of predictive distributions, which are dynamically synthesized and updated to produce coherent forecast distributions. Time-varying biases and interdependencies between data reported at different frequencies are learnt and effectively mapped onto easily interpretable parameters with associated uncertainty. Furthermore, the proposed framework allows for flexible methodological specifications based on policy goals and utility. A macroeconomic study of nowcasting two decades of quarterly US GDP using monthly macroeconomic and financial indicators is presented. In terms of both point and density forecasts, our proposed method significantly outperforms competing methods throughout the quarter, and is competitive with the aggregate Survey of Professional Forecasters. The study further shows that incorporating information during a quarter, and sequentially updating information throughout, markedly improves the performance, while providing timely insights that are useful for decision-making.
Self‐Motion and Cognition: Plato's Theory of the Soul
Authors: Campbell Douglas R.
Published: June 2021

I argue that Plato believes that the soul must be both the principle of motion and the subject of cognition because it moves things specifically by means of its thoughts. I begin by arguing that the soul moves things by means of such acts as examination and deliberation and that this view is developed in response to Anaxagoras. I then argue that every kind of soul enjoys a kind of cognition, with even plant souls having a form of Aristotelian discrimination (krisis), and that there is therefore no completely unintelligent, evil soul in the cosmos that can explain disorderly motions; as a result, the soul is not the principle of all motion but only motion in the cosmos after it has been ordered by the Demiurge.
Don’t miss the forest for the trees: New recommendations for exploiting guilt and shame in self‐control conflicts
Authors: Li Ying , Jia Lile
Published: June 2021

Guilt and shame, two negative self‐conscious emotions, have drawn theoretical and empirical attention in studying self‐control. Although a functionalist view suggests that their reparative tendencies would help combat self‐control failures, the evidence is equivocal. In this review, we begin with a systematic analysis of how the context of self‐control conflicts allows mood management to dominate reparative control as the primary means of regulating guilt and shame and, subsequently, sours the potential benefits of mood management. Then, inspired by the emerging literature of strategic indulgence and a multilevel perspective on self‐control, we propose that people should adopt a tolerant view of indulgence at the behavioral level while channeling the reparative tendencies of guilt and shame at the strategy (behavioral plans) and the system (goal balance) levels. When appraising and regulating self‐control, thus, focusing on the forest rather than the trees may help capitalize on the benefits of guilt and shame.
Hockey Is Not for Everyone, But It Could Be
Authors: Kabetu Victoria , Snelgrove Ryan , Lopez Kimberly J. , Wigfield Daniel
Published: June 2021

Steve Kroger, president and COO of Hockey Canada, is contemplating how to attract and retain more young people who are Black, Indigenous, and People of Color in minor hockey (ages 4–18 years). Hockey Canada the governing association for amateur hockey in the country has created programs that make the sport accessible for more people to try, yet Steve recognizes there is still a lot of work that needs to be done to increase participation rates among Black, Indigenous, and People of Color athletes. Drawing on a policy paper for anti-racism in Canadian hockey, Steve tasks his team with developing strategies aimed at making the sport more inclusive and boosting participation.
Individual Participant Data Meta-Analysis Provides No Evidence of Intervention Response Variation in Individuals Supplementing With Beta-Alanine
Authors: Esteves Gabriel Perri , Swinton Paul , Sale Craig , James Ruth M. , Artioli Guilherme Giannini , Roschel Hamilton , Gualano Bruno , Saunders Bryan , Dolan Eimear
Published: June 2021

Currently, little is known about the extent of interindividual variability in response to beta-alanine (BA) supplementation, nor what proportion of said variability can be attributed to external factors or to the intervention itself (intervention response). To investigate this, individual participant data on the effect of BA supplementation on a high-intensity cycling capacity test (CCT110%) were meta-analyzed. Changes in time to exhaustion (TTE) and muscle carnosine were the primary and secondary outcomes. Multilevel distributional Bayesian models were used to estimate the mean and SD of BA and placebo group change scores. The relative sizes of group SDs were used to infer whether observed variation in change scores were due to intervention or non-intervention-related effects. Six eligible studies were identified, and individual data were obtained from four of these. Analyses showed a group effect of BA supplementation on TTE (7.7, 95% credible interval [CrI] [1.3, 14.3] s) and muscle carnosine (18.1, 95% CrI [14.5, 21.9] mmol/kg DM). A large intervention response variation was identified for muscle carnosine (σIR = 5.8, 95% CrI [4.2, 7.4] mmol/kg DM) while equivalent change score SDs were shown for TTE in both the placebo (16.1, 95% CrI [13.0, 21.3] s) and BA (15.9, 95% CrI [13.0, 20.0] s) conditions, with the probability that SD was greater in placebo being 0.64. In conclusion, the similarity in observed change score SDs between groups for TTE indicates the source of variation is common to both groups, and therefore unrelated to the supplement itself, likely originating instead from external factors such as nutritional intake, sleep patterns, or training status.
Comparison of phenotypical and molecular methods for the identification of bacterial strains isolated from a deep subsurface environment
Authors: Boivin-Jahns V , Bianchi A , Ruimy R , Garcin J , Daumas S , Christen R
Published: June 2021

Vol. 61, no. 9, p. 3406: the Acknowledgments should read as follows. "This work was supported by the CNRS and by grants of the European Community and ANDRA as part of the project Archimede-Argiles and of the Direction de la Recherche et des Etudes Doctorales. V.B. was a recipient of a CIFRE fellowship." [This corrects the article on p. 3400 in vol. 61.].
Salmonella Typhimurium Adhesin OmpV Activates Host Immunity To Confer Protection against Systemic and Gastrointestinal Infection in Mice
Authors: Kaur Deepinder , Gandhi Shraddha , Mukhopadhaya Arunika
Published: June 2021

Salmonella enterica
Typhimurium is a rod-shaped Gram-negative bacterium that mostly enters the human body through contaminated food. It causes a gastrointestinal disorder called salmonellosis in humans and typhoid-like systemic disease in mice.
Oral toxicities of Clostridium botulinum type C and D toxins of different molecular sizes
Authors: Ohishi I , Sakaguchi G
Published: June 2021

Clostridium botulinum type C progenitor toxins of different molecule sizes, C-L (16S) and C-M (12S), were purified from cultures of strains 573, Stockholm, and CB-19. C-L toxin showed some hemaggglutinin activity, whereas C-M toxin did not. Neither C-L nor C-M toxin was activated upon trypsinization. Molecular dissociation of purified type C-L and C-M toxins into toxic and nontoxic components was demonstrated by sucrose density gradient ultracentrifugation and diethylaminoethyl-Sephadex chromatography at pH 8.0. The molecular construction of type C progenitor toxin appears to be analogous to that reported for botulinum toxins of other types. C-L and D-L toxins showed higher oral toxicities to mice than did C-M or D-M toxin. Such higher oral toxicities were ascribed to the higher stabilities of these toxins in gastric and intestinal juices.
Effects of some plant lectins on hydrogen peroxide release from macrophages induced with streptococcal preparation OK-432
Authors: Tomioka H , Saito H
Published: June 2021

Concanavalin A and phytohemagglutinin were found to cause marked inhibition of H2O2 release from macrophages induced with killed streptococci (preparation OK-432). The inhibitory effect of these two lectins on the H2O2 release from macrophages was observed with spontaneous and wheat germ lectin-triggered H2O2 release. This suggests that the lectins act directly on the macrophage H2O2-releasing function, per se, but not on the wheat germ lectin-H2O2 release-enhancing process. Concanavalin A exhibited its inhibitory action on macrophage H2O2 release by specific binding to D-mannopyranoside receptor sites on the macrophage cell surface. Galactose-binding lectins, peanut agglutinin, and soybean agglutinin failed to inhibit, but, on the other hand, slightly enhanced macrophage H2O2 release. The effect of these five lectins on the phagocytosis of latex particles by macrophages was tested. Wheat germ lectin, concanavalin A, and phytohemagglutinin significantly depressed the macrophage phagocytosis, whereas peanut agglutinin and soybean agglutinin failed to show any inhibitory action.
Identification of staphylococcal Panton-Valentine leukocidin as a potent dermonecrotic toxin
Authors: Ward P D , Turner W H
Published: June 2021

The Panton-Valentine leukocidin of Staphylococcus aureus was shown to exhibit a potent dermonecrotic effect when injected intradermally into rabbits. This effect could be abrogated by immunizing animals with the F component or both components, but immunization with the S component appeared to exacerbate certain of the intradermal responses.
Cell-mediated immunoprotection in blastomycosis
Authors: Cozad G C , Chang C T
Published: June 2021

Delayed hypersensitivity can be induced in C57BL/6J mice by two subcutaneous injections of Merthiolate-killed Blastomyces dermatitidis yeast cells in Freund incomplete adjuvant. Development of delayed hypersensitivity peaked at the day 18 post-primary antigen-emulsion injection, as determined by footpad sensitivity tests. Mice rendered hypersensitive to B. dermatitidis were protected from the lethal effect of a blastomyces infection. The protection effects were shown both in mortality tests and in data from organ cultures as expressed by indices of resistance. Data from this study show that there is a close parallel relationship between host resistance and the prevailing level of delayed hypersensitivity.
Delayed Hypersensitivity to
Toxoplasma
and Unrelated Antigens in
Toxoplasma
-Infected Mice: Induction and Elicitation of Delayed-Type Hypersensitivity by Antigen-Pulsed Macrophages
Authors: Handman Emanuela , Chester Patrice M. , Remington Jack S.
Published: June 2021

Delayed-type hypersensitivity (DTH) to
Toxoplasma
and unrelated antigens in
Toxoplasma
-infected BALB/c mice was investigated by the radioisotopic uptake method of Vadas et al. (Int. Arch. Allergy Appl. Immunol.
49:
670-692, 1975). DTH became positive on day 30 of infection and remained positive during chronic infection. The expression of DTH in mice infected with the relatively avirulent C37 strain of the parasite paralleled the
Toxoplasma
antibody response as detected by the Sabin-Feldman dye test. Mice sensitized with
Toxoplasma
, keyhole limpet hemocyanin, or sheep erythrocytes during the acute or chronic phase of
Toxoplasma
infection showed a DTH reaction similar to that of uninfected sensitized controls. No parasite antigens could be detected by immunofluorescence techniques on the surface of
Toxoplasma
-infected cells. When killed organisms were added to the cell cultures, specks of fluorescence appeared on cells containing intracellular parasites as well as on cells without intracellular organisms. That the antigens may be present in or on macrophages in a form readily recognizable by T cells is suggested by experiments in which we demonstrated that injection of uninfected normal macrophages pulsed with
Toxoplasma
-soluble antigens into the ears of chronically infected mice elicited a DTH reaction comparable to that observed when 10
6
Formalin-fixed tachyzoites were used as the test antigen. When macrophages pulsed with
Toxoplasma
antigen were used in attempts to induce DTH in naive uninfected mice, the intensity of the reaction was similar to that observed in infected mice.
Fibril-mediated adherence of Actinomyces viscosus to saliva-treated hydroxyapatite
Authors: Wheeler T T , Clark W B
Published: June 2021

Fibril-mediated adherence of Actinomyces viscosus strain T14V cells to saliva-treated hydroxyapatite was studied. Fibrils were purified by ammonium sulfate precipitation and differential centrifugation from the crude supernatant of whole cells that were sheared by one passage through a French pressure cell. Purified fibrils and crude supernatant inhibited strain T14V adherence to saliva-treated hydroxyapatite to similar extents. However, anti-strain T14V serum and antifibril specific antibody completely abolished strain T14V adherence. The blocking immunoglobulin could be adsorbed from anti-T14V serum by strain T14V whole cells, by purified fibrils, and, to a lesser extent, by cell walls. It was concluded that fibrils mediate adherence of strain T14V cells to saliva-treated hydroxyapatite. In addition, fibril preparations were shown to contain more than 95% protein and to be antigenically homogeneous by immunodiffusion and Laurell rocket immunoelectrophoresis. Purified fibril preparations showed serological identity with the virulence-associated 1 antigen of Lancefield-extracted T14V cells, whereas crude supernatants contained both virulence-associated 1 and virulence-associated 2 antigens, as shown by rocket immunoelectrophoresis.
Peripheral infection in culture of rat sensory neurons by herpes simplex virus
Authors: Ziegler R J , Herman R E
Published: June 2021

The peripheral infection of neurons from dissociated rat sensory ganglia by herpes simplex virus was studied with a newly developed tissue culture system. The results indicated that the virus arrives at the neuronal soma by an axoplasmic route and that at least some of these neurons are productively infected since eventually all cells in the culture appeared to be destroyed by the virus. This system showed promise in elucidating the possible roles of various effector cells, antiviral antibody, or antiviral compounds in the mechanism of herpes simplex virus latency.
Plague virulence antigens from Yersinia enterocolitica
Authors: Carter P B , Zahorchak R J , Brubaker R R
Published: June 2021

The virulence of Yersinia enterocolitica, biotype 2, serotype O:8, in mice is related to its ability to produce plague V and W antigens. V and W antigens in Y. enterocolitica are shown to be immunologically identical to the previously described V and W antigens of Yersinia pestis and Yersinia pseudotuberculosis.
Lysis and protoplast formation of group B streptococci by mutanolysin
Authors: Calandra G B , Cole R M
Published: June 2021

Group B streptococci, refractory to previously tested muralysins under physiological conditions, were successfully converted to protoplasts by use of a recently describede N-acetyl muramidase, mutanolysin, derived from a streptomycete. Purified enzyme was effective, but crude preparations, although degrading cell walls, simultaneously produced peculiar effects of cytoplasmic coagulation, retention of cell shape, loss of some intracellular enzymes, and a rise in optical density. Addition of purified mutanolysin to the array of muralysins (group C streptococcal phage-associated lysin, lysozyme), previously successful in preparing protoplasts of different streptococci, now makes possible enzymatic preparation of protoplasts of streptococci of groups A, B, C. D. G, and H.
Physiochemical properties of a heat-stable enterotoxin produced by Escherichia coli of human origin
Authors: Madsen G L , Knoop F C
Published: June 2021

Concentrated cell-free filtrates, prepared from a human strain of enterotoxigenic Escherichia coli, were subjected to isoelectric focusing, molecular sieve chromatography, and polyacrylamide disc gel electrophoresis. Isoelectric focusing in a pH 3 to 5 gradient resulted in two biologically active peaks, I and II, that electrofocused at pI 1.5 and 3.8, respectively. Molecular sieve chromatography of the major enterotoxic peak (II) at pH 3.8 indicated a molecular weight of 2,500. Polyacrylamide disc gel electrophoresis of ST revealed a single protein band containing enterotoxic activity.
In vivo evaluation of pathogenicity of clinical and environmental isolates of Vibrio cholerae
Authors: Sigel S P , Lanier S , Baselski V S , Parker C D
Published: June 2021

Thirty-three minimally passaged clinical and environmental isolates of Vibrio cholerae were examined for ability to survive and multiply in the upper bowel of infant mice and to elicit diarrhea. All of 21 smooth O-1 V. cholerae isolates from stool were able to multiply and elicit diarrhea. Three rough strains isolated from stool were unable to multiply or to elicit diarrhea. Two smooth O-1 isolates associated with cholera cases (from a sewer and a septic tank) also were able to cause disease. However, four O-1 strains and one non-O-1 strain from sources not associated with cholera cases did not cause mouse disease. A human gall bladder isolate was also avirulent, whereas a Louisiana shrimp isolated showed low mouse virulence. We conclude that smooth human diarrheal isolates of V. cholerae of serogroup O-1 are virulent for infant mice. Examination of sequential isolates from single patients showed that some strains isolated later in infection had a reduced ability to induce diarrhea. Comparison of epidemiologically related strains showed that an isolate from crab had a low ability to induce disease in infant mice, whereas the isolates from patients showed the expected ability to multiply and elicit diarrhea in mice.
Induction of 2',5'-oligoadenylate synthetase and interferon in mouse trigeminal ganglia infected with herpes simplex virus
Authors: Sokawa Y , Ando T , Ishihara Y
Published: June 2021

The activity of 2',5'-oligoadenylate synthetase, which is induced by interferon action, was detected in mouse trigeminal ganglia infected with herpes simplex virus (HSV) type 1. When HSV was inoculated on the left cheek of mice, the virus began to appear in the ipsilateral trigeminal ganglia on day 2, reached its maximum accumulation on day 4, declined thereafter, and was no longer detected in the tissue homogenate after 11 days. After this short acute productive phase, the virus entered into the latent phase of infection. 2',5'-Oligoadenylate synthetase appeard in the trigeminal ganglia shortly after the beginning of virus multiplication; the synthetase activity began to rise on day 3, reached a maxium level on day 4, and then declined. Interferon activity (type I) also appeared in the infected ganglia, but diminished more rapidly than the synthetase. The antibody against HSV in the sera began to appear at the time when the virus and synthetase were declining. From these results it may be hypothesized, although not concluded, that in the acute productive phase of HSV infection in mouse trigeminal ganglia, virus multiplication is suppressed by interferon action, including 2',5'-oligoadenylate synthetase induction.
Resistance of Exotoxin A to Purified Pseudomonas Proteolytic Enzymes
Authors: Jagger Kathleen , Nickol Michele M. , Saelinger Catharine B.
Published: June 2021

Pseudomonas toxin is produced as a proenzyme which is cytotoxic for cells in culture but must be activated to express full enzymatic activity. The ability of purified pseudomonas alkaline protease and elastase or of culture filtrates of two strains of
Pseudomonas aeruginosa
to modify the activity of pseudomonas toxin was examined. Two parameters of toxin activity were followed: enzymatic activity, i.e., the adenosine diphosphate (ADP) ribosylation of elongation factor 2, and biological activity, i.e., inhibition of protein synthesis in cultured mouse fibroblasts. Biological activity of toxin depends upon an intact toxin molecule, whereas enzyme activity requires only a functional A region. Incubation with purified pseudomonas proteolytic enzymes did not alter either enzymatic or biological activity. The toxin is not refractory to the action of all proteolytic enzymes, since thermolysin rapidly destroyed the toxin molecule. Treatment of toxin with culture filtrates of
P. aeruginosa
reduced ADP ribosylation activity, but increased the ability of toxin to inhibit protein synthesis in cell monolayers. Incubation of culture filtrates with one of the protease inhibitors alpha-2-macroglobulin or phosphoramidon did not alter the effect of the filtrates on biological activity. Alpha-2-macroglobulin, however, caused a fourfold stimulation of ADP ribosylation activity of the toxin. We conclude that pseudomonas alkaline protease and elastase are not responsible for the modifications in toxin activity induced by culture filtrates of
P. aeruginosa
; the factors responsible have not yet been identified, but are not inactivated by phosphoramidon or alpha-2-macroglobulin.
Temperature-Sensitive Mutants of Influenza A Virus: Transfer of the Two Temperature-Sensitive Lesions in the Udorn/72-
ts
-1A2 Virus to the A/Hong Kong/123/77 (H1N1) Wild-Type Virus
Authors: Murphy Brian R. , Hosier Nanette T. , Chanock Robert M.
Published: June 2021

The influenza A/Udorn/72-
ts
-1A2 virus possesses temperature-sensitive mutations in the genes coding for the P1 and P3 polymerase proteins. It is being evaluated as a donor of its attenuating temperature-sensitive genes to produce recombinant live vaccine strains of epidemic variants of influenza A virus. Transfer of the P1 and P3 genes to two viruses within the H3N2 subtype of influenza A virus (i.e., the A/Victoria/3/75 and A/Alaska/6/77 viruses) conferred on each variant the following properties: (i) 37°C shutoff temperature for plaque formation, (ii) almost complete restriction of viral replication in the lungs, (iii) a 100-fold restriction of viral replication in the nasal turbinates, and (iv) genetic stability after replication in hamsters. This study was undertaken to determine whether the transfer of the two
ts
-1A2 temperature-sensitive genes into a virus belonging to the H1N1 subtype (i.e., the A/Hong Kong/123/77 virus) would result in a restriction of replication in vitro and in vivo comparable to that observed with the previously studied H3N2 recombinant viruses in hamsters. This was found to be the case. In addition, infection of hamsters with the A/Hong Kong/77-
ts
-1A2 virus induced significant resistance to infection with wild-type A/Hong Kong/77 virus. Thus, the two
ts
-1A2 temperature-sensitive genes attenuated influenza A viruses belonging to two distinct subtypes to a specific and predictable level. An unexpected genetic interaction was observed between several A/Hong Kong/77-
ts
-1A2 segregants bearing the group 5 (P1) temperature-sensitive lesion. One interpretation of these results is that intracistronic complementation occurred between these segregants.
Induction of cytolytic T- and B-cell responses against influenza virus infections
Authors: Armerding D , Rossiter H
Published: June 2021

Inoculation of mice with live influenza virus results in the induction of cytotoxic thymus-derived (T) lymphocytes and of bone marrow-derived (B) cells producing antiviral antibodies. An assay system was developed to evaluate both types of immune responses on a cellular basis within the same lymphocyte pool with no need to separate out the different effector cell classes. The test system represented a modification of the 51Cr-release assay. T-cell activity was measured exclusively in the absence of active complement using targets that were compatible for determinants encoded by the mouse major histocompatibility gene complex, H-2. H-2-different and even xenogeneic target cells were lysed in the presence of either non-inactivated fetal calf serum or normal rabbit serum as a complement source. Cytotoxicity was mediated in the latter case by direct interaction of B-cell-produced immunoglobulin directed to viral antigens expressed by the target cell and complement. Antibody-dependent cell-mediated cytotoxicity mechanisms did not contribute to cytotoxicity in the test system described. It was demontrated that the cytolytic B-cell responses of one particular strain of mice (BALB/c) against different influenza A viruses were restricted to the immunizing virus on the effector cell level. In another strain of mice (C3H), B cells revealed a broad cross-reactive response resembling that of killer T cells.
Murine Trypanosomiasis: Cellular Proliferation and Functional Depletion in the Blood, Peritoneum, and Spleen Related to Changes in Bone Marrow Stem Cells
Authors: Clayton Christine E. , Selkirk Murray E. , Corsini Carlos A. , Ogilvie Bridget M. , Askonas Brigitte A.
Published: June 2021

Previous reports have described profound effects on the function of the lymphoid system, especially the spleen, in mice infected with
Trypanosoma brucei
. This study provides further evidence of major change in the cell populations of the blood, peritoneum, and bone marrow, but shows that at least some of the stem cells of the bone marrow survive the damage caused by trypanosomes and retain their ability to repopulate the animal. In these infected mice the initial parasitemia was terminated by day 11 and was followed by a subpatent period of approximately 7 days before a final, lethal parasitemia occurred. Lymphopenia preceded the initial and final waves of parasites in the blood, and there was a marked increase in circulating neutrophils and large mononuclear cells for 1 week after the termination of the first wave of bloodstream parasitemia and during the final lethal parasitemia. Dividing macrophages were detected in the peritoneum only briefly during week 1 of infection, but the total number of peritoneal cells was increased from day 8 until the mice died. The bone marrow is severely stressed by the parasite infection. Total cell numbers and spleen colony-forming cells in the bone marrow were profoundly depleted during the resolution of the first parasitemia, but both these parameters largely recovered during the subpatent period before the mice were killed by the disease. Immune function was restored gradually after treatment with Berenil late in infection. We conclude that the progenitors of lymphocytes as well as the mature cells are affected by trypanosomes, but that some of the early bone marrow stem cells escape and rapidly repopulate the peripheral organs upon removal of the parasites.
Serum immunoconglutinin titers during acute and chronic hepatitis B virus infection
Authors: Makhdoomi G M , Tiku M L , Beutner K R , Ogra P L
Published: June 2021

Sera from 99 chronic hepatitis B surface antigen carriers, 12 individuals with acute type B hepatitis, 26 hepatitis B surface antibody-seropositive subjects, and 50 hepatitis B surface antigen, hepatitis B surface antibody-seronegative subjects were evaluated for the presence of serum imunoconglutinis (IKs). The mean serum IK titers of hepatitis B surface antibody-seropositive and hepatitis B virus-seronegative subjects wre 5.3 and 4.9, respectively. The IK titers of subjects with acute and chronic hepatitis B virus infections were 215.4 and 19.1, respectively. These groups also manifested IK titers greater than or equal to > 16 significantly (P < 0.005) more often than controls did. Among chronic hepatitis B surface antigen carriers, high IK titers were associated with low levels of hepatitis B surface antigen. IK titers of individuals chronically infected with hepatitis B virus and having the rheumatoid factor were similar to those of individuals without the rheumatoid factor. Elevated IK titers represent a physiological autoimmune response and may indicate the presence of immune complexes in acute and chronic hepatitis B virus infection.
Immunotherapy of experimental cancer by intralesional injection of emulsified nonliving mycobacteria: comparison of Mycobacterium bovis (BCG), Mycobacterium phlei, and Mycobacterium smegmatis
Authors: Yarkoni E , Rapp H J
Published: June 2021

Mycobacterium bovis (BCG), Mycobacterium phlei, and Mycobacterium smegmatis were each tested in emulsified form for their potency to cause regression of transplants of a syngeneic murine fibrosarcoma and of a syngeneic guinea pig hepatoma. On a weight basis, M. phlei and M. smegmatis were as effective as BCG in causing tumor regression. M. phlei and M. smegmatis were comparable to BCG in provoking delayed cutaneous hypersensitivity reactions in guinea pigs sensitized to M. phlei or M. smegmatis. In BCG-sensitized guinea pigs, M. phlei and M. smegmatis provoked weaker delayed cutaneous hypersensitivity reactions than did BCG. Purified protein derivative of M. tuberculosis was more active in eliciting delayed cutaneous hypersensitivity in BCG-sensitized guinea pigs than in animals sensitized with M. phlei or M. smegmatis.
Adjuvant Activity of a Novel Metabolizable Lipid Emulsion with Inactivated Viral Vaccines
Authors: Reynolds J. A. , Harrington D. G. , Crabbs C. L. , Peters C. J. , Di Luzio N. R.
Published: June 2021

Studies were conducted in mice, hamsters, sheep, and two species of nonhuman primates which demonstrate the adjuvant activity of a new metabolizable lipid emulsion with marginally immunogenic doses of Formalin-inactivated viral vaccines. The lipid base consists of highly refined peanut oil emulsified in aqueous vaccines with glycerol and lecithin. Hamsters and mice inoculated with lipid emulsion plus western or Venezuelan equine encephalitis vaccine were significantly more resistant than vaccinated controls to lethal homologous virus challenge. Sheep given one dose of lipid emulsion plus Rift Valley fever vaccine developed significantly higher antibody titers than control sheep receiving only vaccine. Cynomolgous monkeys inoculated with lipid emulsion plus Rift Valley fever vaccine developed 16-fold greater peak primary and 20-fold greater secondary antibody titers than those of vaccine controls. Similar lipid emulsion-Rift Valley fever studies in rhesus monkeys resulted in 37- and 300-fold increases in primary and secondary titers, respectively, compared with monkeys given vaccine alone. Neither the sequence of combining antigen with lipid nor the exact ratio of aqueous phase to lipid phase affected the survival of Venezuelan equine encephalitis-vaccinated mice challenged with homologous lethal virus. This lipid formulation has several advantages over other water-in-oil adjuvants for potential use in humans. The components are metabolizable or normal host constituents, it is easily emulsified with aqueous vaccines by gentle agitation, and it is relatively nonreactogenic in recipients.
Complement activation by Coccidioides immitis: in vitro and clinical studies
Authors: Galgiani J N , Yam P , Petz L D , Williams P L , Stevens D A
Published: June 2021

Mycelial- or spherule-phase derivatives of Coccidioides immitis caused a decrease in vitro of total hemolytic complement in serum from a nonsensitized person. Activation involved both classic and alternative pathways as shown by deprssion of hemolytic C4 and by generation of products of activation of components C3, C4, and factor B. In addition, functional complement activity or immunoreactive levels of complement components or both were measured in 23 patients with self-limited or disseminated coccidioidomycosis. Low total hemolytic complement was found in nine, usually during the early phase of primary illness, and was transient. Hemolytic C4 was low, and the effect of inulin to decrease complement levels was blunted, suggested both classic and alternative pathways may be deficient. However, associated depression of immunoreactive levels of components assayed (C3, C4, C5, factor B, and properdin) was not consistently found. This disparity raises the possibility of enhanced in vitro inactivation analogous to activation by immune complexes.
In vitro phagocytosis of Candida albicans by peritoneal mouse macrophages
Authors: Evron R
Published: June 2021

The ability of sensitized mouse peritoneal macrophages to phagocytose and inhibit Candida albicans was studied in an in vitro system. Mice were sensitized to C. albicans by intraperitoneal infection with viable organisms or by intracutaneous injection of heat-inactivated cells in Freund complete adjuvant. Development of delayed hypersensitivity to C. albicans was evaluated by footpad tests with cytoplasmic and cell wall antigens as well as by macrophage migration inhibition by these antigens and by whole heat-inactivated cells. Inhibition of macrophage migration by heat-inactivated cells was significantly greater when the mice were sensitized by viable organisms. The macrophages from these mice were also larger and showed a greaer ability to inhibit germ tube production by phagocytosed yeasts. This suggests that macrophages may play a protective role in infection by C. albicans.