bluuebunny/crossref_metadata_2025 huggingface dataset:
Optimal Design of Formulas for a Single Degree of Freedom Tuned Mass Damper Parameter Using a Genetic Algorithm and H2 Norm
Authors: Kim Seunggoo , Lee Donwoo , Lee Seungjae
Published: July 2024

One of the researchers’ concerns in structural engineering is to control the dynamic behavior of structures efficiently. The TMD (tuned mass damper) is one of the effective methods of controlling the vibration of structures, and various numerical techniques have been proposed to find the optimal parameters of the TMD. This paper develops a new explicit formula to derive the optimal parameters of the TMD of a single degree of freedom (SDOF) structure under seismic load using a genetic algorithm (GA). In addition, the state-space model and the H2 norm function are used to identify the optimal frequency ratio and damping ratio of the TMD that minimize the overall vibration energy of the structure. The MATLAB curve fitting toolbox is used for the explicit formula proposal, and the validity of the proposed formula is verified through multidimensional performance verification technique. Finally, the TMD parameters of the SDOF structure are applied to the multi-degrees of freedom (MDOF) structure to compare and analyze with the existing research results, and the results of the explicit formula proposed in this paper are confirmed to be excellent. This paper can suggest a new direction for determining the optimal TMD parameters using a GA and can be effectively applied to vibration control problems of various structures.
Investigating the Influence of Counterflow Regions on the Hydrodynamic Performance of Biomimetic Robotic Fish
Authors: Gong Yanling , Wang Ming , Zhao Qianchuan , Wang Ruilong , Zuo Lingchen , Zheng Xuehan , Gao He
Published: July 2024

Biomimetic robotic fish are a novel approach to studying quiet, highly agile, and efficient underwater propulsion systems, attracting significant interest from experts in robotics and engineering. These versatile robots showcase their ability to operate effectively in various water conditions. Nevertheless, the comprehension of the swimming mechanics and the evolution of the flow field of flexible robots in counterflow regions is still unknown. This paper presents a framework for the self-propulsion of robotic fish that imitates biological characteristics. The method utilizes computational fluid dynamics to analyze the hydrodynamic efficiency of the organisms at different frequencies of tail movement, under both still and opposing flow circumstances. Moreover, this study clarifies the mechanisms that explain how changes in the aquatic environment affect the speed and efficiency of propulsion. It also examines the most effective swimming tactics for places with counterflow. The results suggest that the propulsion effectiveness of robotic fish in counterflow locations does not consistently correspond to various tail-beat frequencies. By utilizing vorticity maps, a comparative analysis can identify situations when counterflow zones improve the efficiency of propulsion.
Far-Red Fluorescent Proteins: Tools for Advancing In Vivo Imaging
Authors: Shang Angyang , Shao Shuai , Zhao Luming , Liu Bo
Published: July 2024

Far-red fluorescent proteins (FPs) have emerged as indispensable tools in in vivo imaging, playing a pivotal role in elucidating fundamental mechanisms and addressing application issues in biotechnology and biomedical fields. Their ability for deep penetration, coupled with reduced light scattering and absorption, robust resistance to autofluorescence, and diminished phototoxicity, has positioned far-red biosensors at the forefront of non-invasive visualization techniques for observing intracellular activities and intercellular behaviors. In this review, far-red FPs and their applications in living systems are mainly discussed. Firstly, various far-red FPs, characterized by emission peaks spanning from 600 nm to 650 nm, are introduced. This is followed by a detailed presentation of the fundamental principles enabling far-red biosensors to detect biomolecules and environmental changes. Furthermore, the review accentuates the superiority of far-red FPs in multi-color imaging. In addition, significant emphasis is placed on the value of far-red FPs in improving imaging resolution, highlighting their great contribution to the advancement of in vivo imaging.
You’re Beautiful When You Smile: Event-Related Brain Potential (ERP) Evidence of Early Opposite-Gender Bias in Happy Faces
Authors: Schmuck Jonas , Voltz Emely , Gibbons Henning
Published: July 2024

Studies of social cognition have shown gender differences regarding human face processing. One interesting finding is the enhanced processing of opposite-gender faces at different time stages, as revealed by event-related brain potentials. Crucially, from an evolutionary perspective, such a bias might interact with the emotional expression of the face. To investigate this, 100 participants (50 female, 50 male) completed an expression-detection task while their EEG was recorded. In three blocks, fearful, happy and neutral faces (female and male) were randomly presented, with participants instructed to respond to only one predefined target expression level in each block. Using linear mixed models, we observed both faster reaction times as well as larger P1 and late positive potential (LPP) amplitudes for women compared to men, supporting a generally greater female interest in faces. Highly interestingly, the analysis revealed an opposite-gender bias at P1 for happy target faces. This suggests that participants’ attentional templates may include more opposite-gender facial features when selectively attending to happy faces. While N170 was influenced by neither the face nor the participant gender, LPP was modulated by the face gender and specific combinations of the target status, face gender and expression, which is interpreted in the context of gender-emotion stereotypes. Future research should further investigate this expression and attention dependency of early opposite-gender biases.
Influence of Manufactured Sand on Fresh Properties, Strength Properties and Morphological Characteristics of Self-Compacting Coconut Shell Concrete
Authors: Prasanth Suresh , Chandar Sekaran Prakash , Gunasekaran Kandasamy
Published: July 2024

This research examines the fresh properties, strength performance, and morphological analysis of self-compacting coconut shell concrete (SCCSC) blended with crushed coconut shell and manufactured sand (M-sand). Crushed coconut shell (CS) was used as a coarse aggregate (CA), and M-sand replaced river sand (R-sand) at 25%, 50%, 75%, and 100%. The study focused on the workability characteristics, mechanical behavior, and microstructural analysis of SCCSC. Experiments were performed on fresh and mechanical characteristics, including slump flow diameter, T500 slump flow time, L-Box blocking ratio, V-funnel and a wet sieving stability test. Mechanical characteristics include compressive, split tensile, flexural, impact resistance and bond strength. Utilizing M-sand develops the mechanical performance of SCCSC. The morphological characteristics, using scanning electron microscopy (SEM), energy-dispersive spectroscopy (EDS), Fourier transform infrared (FTIR) and the X-ray diffraction (XRD) technique, were studied in this research work. The findings show that the addition of M-sand increases the concrete strength. The microstructural analysis demonstrates that adding different amounts of M-sand to SCCSC reduced the porosity and anhydrous cement percentage, although it increased calcium hydroxide and hydration products. The substitution of 100% M-sand at 28 days increased compressive strength by 3.79% relative to the reference SCCSC. Based on the findings, the mechanical strength of SCCSC containing M-sand significantly improved compared to the concrete with river sand.
Research on the Reinforcement Characteristics of Thick Cushion Layer and Rigid Pile Composite Foundation
Authors: Chen Junhua , Nie Yuzhi , Lian Guan , Chen Aijun , Pu Siqi , Zou Jinfeng , Zhang Jiasheng , Shi Xiong , Wu Di , Yang Bai
Published: July 2024

The rigid pile composite foundation method is the most commonly used method for strengthening weak soil foundations. In this method, piles usually need to pass through weak soil layers, and the pile end falls on a bearing layer with good bearing capacity. Under existing technical conditions, the thicker the weak soil layer, the longer the pile body, and the more difficult it is to ensure the construction quality of the pile. In response to this issue, some scholars have adopted the rigid pile composite foundation method with a thick cushion layer for reinforcement treatment. This article uses PLAXIS 3D (V20.04.00.790) software to establish a finite element model of rigid pile composite foundation with a thick cushion layer and simulate the process of foundation reinforcement. The influence of parameters such as thickness, compression modulus, and shear strength index of the cushion layer on foundation settlement and pile–soil stress distribution is studied, and the reasonable range of these parameters is analyzed under the condition of considering reinforcement effect. Through comparative analysis, it can be concluded that for deep and weak soil areas, the thickness of the cushion layer can range from 0.5 to 2.6. The thickness and compressive modulus of the cushion layer have a significant impact on the settlement of the foundation, the pile–soil stress ratio, and the stress of the pile body, while the shear strength index of the cushion layer has a relatively small impact on these parameters. Reasonably selecting the geometric and mechanical parameters of the cushion layer can effectively reduce stress concentration at the pile top and better play the role of the soil between piles.
Modeling Workplace Well-Being Factors in Infrastructure Construction Projects: PLS-SEM Approach
Authors: Alhammadi Yasir , Radzi Afiqah R. , Alias Ahmad Rizal , Rahman Rahimi A.
Published: July 2024

Working at construction sites can harm individuals’ health and well-being. However, stakeholders often focus on improving health while discounting well-being. Establishing a better workplace environment can improve workplace well-being. Therefore, this study aims to investigate the factors influencing workplace well-being in infrastructure construction projects. To achieve this aim, the study objectives are to (1) identify the critical factors influencing workplace well-being in infrastructure construction projects; (2) develop an interrelationship model between underlying constructs of the factors; and (3) assess the influence of the underlying constructs on workplace well-being in infrastructure construction projects. First, a list of factors influencing workplace well-being was established using data from a systematic literature review and semi-structured interviews. Then, a survey was developed using the list of factors and distributed to infrastructure construction practitioners. In total, 124 responses were retrieved and analyzed using normalized mean analysis, exploratory factor analysis (EFA) and partial least square structural equation modeling (PLS-SEM). The analyses reveal that the critical factors include workload, salary package, the timeline of salary payment, working hours, planning of the project, insurance for construction workers, workers’ welfare and project progress. Furthermore, the underlying constructs are physical factors and psychosocial factors. Finally, both physical and psychosocial factors are found to significantly influence workplace well-being in infrastructure construction projects. Focusing solely on physical factors may not sufficiently enhance workplace well-being in construction projects, as there are also non-visible factors, such as psychosocial factors. Thus, exploring the interplay between visible and non-visible factors and their influence on workplace well-being in construction projects is crucial. Nevertheless, the findings have several limitations, including using data from a single country and non-hands-on construction practitioners, as well as the underlying constructs being derived solely using statistical methods. Still, the study is original in its focus on modeling the relationship between the factors influencing workplace well-being within the context of infrastructure construction projects. In other words, this study differs from prior research by specifically modeling the factors that influence well-being in infrastructure construction projects.
Bond Behavior of High-Strength Steel Rebar in Ultra-High-Performance Manufactured Sand Concrete: Experiment and Modelling
Authors: Wang Caiqin , Jiao Yubo , Xing Jian , Chen Yaojia
Published: July 2024

Manufactured sand (MS), due to its wide availability and cost-effectiveness, is used as an alternative aggregate for quartz sand (QS) in ultra-high-performance concrete (UHPC) to prepare ultra-high-performance manufactured sand concrete (UHPMC). This study aims to assess the bond behavior of 600 MPa-grade, high-strength, hot-rolled ribbed bars (HRB 600) in UHPMC. Thirty specimens were designed for the pull-out tests, taking into account several feature parameters, including MS replacement ratio (0%, 50%, 100%), water–binder ratio (0.17, 0.19, 0.21), steel fiber content (0%, 1%, 2%), and anchorage length (2d, 3d, 4d, 5d). The effects of the feature parameters on the failure mode, bond stress–slip curves, bond strength, bond-slip mechanism, and constitutive model were analyzed and illustrated. The results reveal that the pull-out specimen of UHPMC exhibits three distinct failure modes: rebar pull-out failure, UHPMC splitting failure, and splitting-pull-out failure. The bond strength increases from 46.57 MPa to 56.92 MPa when the steel fiber content increases from 0% to 2%. Additionally, a decrease in anchoring length is beneficial for improving the bond strength; as the anchoring length increases from 2d to 5d, the bonding strength decreases by 35.84%. The bond strength increases with an increase in the MS replacement ratio. As for the water–binder ratio, the bond strength presents the highest value when the water–binder ratio is 0.17. In addition, a new bond-slip constitutive model applicable to UHPMC and HRB 600 rebar, considering the MS replacement ratio, the water–binder ratio, etc., is proposed, which presents favorable prediction accuracy.
Novel Detection of Pleomorphic Adenomas via Analysis of 68Ga-DOTATOC PET/CT Imaging
Authors: Johnson Felix , Kloppenburg Marcel , Hofauer Benedikt , Wollenberg Barbara , Hoch Cosima C. , Stögbauer Fabian , Haller Bernhard , Knopf Andreas , Strassen Ulrich , Notohamiprodjo Susan
Published: July 2024

Introduction: Currently, the diagnosis of salivary gland tumors using imaging techniques is unreliable. Methods: In this monocentric retrospective study, we examined patients who received a 68Ga-DOTATOC PET/CT and subsequently underwent a salivary gland tumor resection between 1 January 2010 and 31 December 2021. PET/CT image assessment was compared with somatostatin receptor (SSTR) expression and histology. Results: Thirteen patients (five pleomorphic adenoma (PA) and eight other parotid lesions (OPL)) received a 68Ga-DOTATOC PET/CT. Imaging displayed strong focal tracer uptake in all PA except for one with strong tumor to background discrimination. PA revealed higher SUVmax, SUVmean, liver and blood pool quotients than those of Warthin tumors (WT) and of OPL. In comparison to the contralateral parotid, SUVmax (p = 0.02), SUVmean (p = 0.02), liver quotient (p = 0.03) and blood pool quotient (p = 0.03) were all significantly higher. In contrast, WT and OPL showed in relation to the contralateral parotid no significant differences of SUVmax (WT p = 0.79; OPL p = 0.11), SUVmean (WT p = 1.0; OPL p = 0.08), liver quotient (WT p = 0.5; OPL p = 0.08) and blood pool quotient (WT p = 0.8; OPL p = 0.19). Two PA and one granuloma were not available for examination. In the immunohistochemal analysis, all PA demonstrated the highest intensity of SSTR2 expression (grade 3). Furthermore, PA had a high percentage of cells expressing SSTR2 (20%, 80% and 55%). Conclusions: A strong tracer uptake in PA was shown in 68Ga-DOTATOC PET/CT. This may allow physicians to utilize radioligated somatostatin analogue PET CT/MR imaging to accurately diagnose PA. Additionally, it may be possible in the future to treat the PA with a noninvasive peptide receptor radionuclide therapy or with somatostatin analogues.
DNA Damage Response in Early Breast Cancer: A Phase III Cohort in the Phobos Study
Authors: Krasniqi Eriseld , Ercolani Cristiana , Di Benedetto Anna , Di Lisa Francesca Sofia , Filomeno Lorena , Arcuri Teresa , Botti Claudio , Pelle Fabio , Cavicchi Flavia , Cappelli Sonia , Barba Maddalena , Pizzuti Laura , Maugeri-Saccà Marcello , Moscetti Luca , Grassadonia Antonino , Tinari Nicola , Sanguineti Giuseppe , Takanen Silvia , Fragnito Davide , Terrenato Irene , Buglioni Simonetta , Perracchio Letizia , Latorre Agnese , De Maria Ruggero , Pallocca Matteo , Ciliberto Gennaro , Giotta Francesco , Vici Patrizia
Published: July 2024

We assessed the impact of DNA damage response and repair (DDR) biomarker expressions in 222 node-positive early breast cancer (BC) patients from a previous Phase III GOIM 9902 trial of adjuvant taxanes. At a median follow-up of 64 months, the original study showed no disease-free survival (DFS) or overall survival (OS) differences with the addition of docetaxel (D) to epirubicine-cyclophosphamide (EC). Immunohistochemistry was employed to assess the expression of DDR phosphoproteins (pATM, pATR, pCHK1, γH2AX, pRPA32, and pWEE1) in tumor tissue, and their association with clinical outcomes was evaluated through the Cox elastic net model. Over an extended follow-up of 234 months, we confirmed no significant differences in DFS or OS between patients treated with EC and those receiving D → EC. A DDR risk score, inversely driven by ATM and ATR expression, emerged as an independent prognostic factor for both DFS (HR = 0.41, p < 0.0001) and OS (HR = 0.61, p = 0.046). Further validation in a public adjuvant BC cohort was possible only for ATM, confirming its protective role. Overall, our findings confirm the potential role of the DDR pathway in BC prognostication and in shaping treatment strategies advocating for an integrated approach, combining molecular markers with clinical–pathological factors.
Adiponectin Receptor Agonist AdipoRon Inhibits Proliferation and Drives Glycolytic Dependence in Non-Small-Cell Lung Cancer Cells
Authors: Kafeel Sanober , Ragone Angela , Salzillo Alessia , Palmiero Giuseppina , Naviglio Silvio , Sapio Luigi
Published: July 2024

Despite the countless therapeutic advances achieved over the years, non-small-cell lung cancer (NSCLC) is the leading cause of cancer-related death worldwide. To this primacy contribute both non-oncogene addicted and advanced NSCLCs, in which conventional therapies are only partially effective. The adiponectin receptor agonist AdipoRon has revealed antiproliferative action in different cancers, including osteosarcoma and pancreatic cancer. Herein, we investigated its potential anticancer role in NSCLC for the first time. We proved that AdipoRon strongly inhibits viability, growth and colony formation in H1299 and A549 NSCLC cells, mainly through a slowdown in cell cycle progression. Along with the biological behaviors, a metabolic switching was observed after AdipoRon administration in NSCLC cells, consisting of higher glucose consumption and lactate accumulation. Remarkably, both 2-Deoxy Glucose and Oxamate glycolytic-interfering agents greatly enhanced AdipoRon’s antiproliferative features. As a master regulator of cell metabolism, AMP-activated protein kinase (AMPK) was activated by AdipoRon. Notably, the ablation of AdipoRon-induced AMPK phosphorylation by Compound-C significantly counteracted its effectiveness. However, the engagement of other pathways should be investigated afterwards. With a focus on NSCLC, our findings further support the ability of AdipoRon in acting as an anticancer molecule, driving its endorsement as a future candidate in NSCLC therapy.
Depicting Biomarkers for HER2-Inhibitor Resistance: Implication for Therapy in HER2-Positive Breast Cancer
Authors: Cai Alvan , Chen Yuan , Wang Lily S. , Cusick John K. , Shi Yihui
Published: July 2024

HER2 (human epidermal growth factor receptor 2) is highly expressed in a variety of cancers, including breast, lung, gastric, and pancreatic cancers. Its amplification is linked to poor clinical outcomes. At the genetic level, HER2 is encoded by the ERBB2 gene (v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2), which is frequently mutated or amplified in cancers, thus spurring extensive research into HER2 modulation and inhibition as viable anti-cancer strategies. An impressive body of FDA-approved drugs, including anti-HER2 monoclonal antibodies (mAbs), antibody–drug conjugates (ADCs), and HER2-tyrosine kinase inhibitors (TKIs), have demonstrated success in enhancing overall survival (OS) and disease progression-free survival (PFS). Yet, drug resistance remains a persistent challenge and raises the risks of metastatic potential and tumor relapse. Research into alternative therapeutic options for HER2+ breast cancer therefore proves critical for adapting to this ever-evolving landscape. This review highlights current HER2-targeted therapies, discusses predictive biomarkers for drug resistance, and introduces promising emergent therapies—especially combination therapies—that are aimed at overcoming drug resistance in the context of HER2+ breast cancer.
Growth Hormone Upregulates Melanoma Drug Resistance and Migration via Melanoma-Derived Exosomes
Authors: Kulkarni Prateek , Basu Reetobrata , Bonn Taylor , Low Beckham , Mazurek Nathaniel , Kopchick John J.
Published: July 2024

Drug resistance in melanoma is a major hindrance in cancer therapy. Growth hormone (GH) plays a pivotal role in contributing to the resistance to chemotherapy. Knocking down or blocking the GH receptor has been shown to sensitize the tumor cells to chemotherapy. Extensive studies have demonstrated that exosomes, a subset of extracellular vesicles, play an important role in drug resistance by transferring key factors to sensitize cancer cells to chemotherapy. In this study, we explore how GH modulates exosomal cargoes from melanoma cells and their role in drug resistance. We treated the melanoma cells with GH, doxorubicin, and the GHR antagonist, pegvisomant, and analyzed the exosomes released. Additionally, we administered these exosomes to the recipient cells. The GH-treated melanoma cells released exosomes with elevated levels of ABC transporters (ABCC1 and ABCB1), N-cadherin, and MMP2, enhancing drug resistance and migration in the recipient cells. GHR antagonism reduced these exosomal levels, restoring drug sensitivity and attenuating migration. Overall, our findings highlight a novel role of GH in modulating exosomal cargoes that drive chemoresistance and metastasis in melanoma. This understanding provides insights into the mechanisms of GH in melanoma chemoresistance and suggests GHR antagonism as a potential therapy to overcome chemoresistance in melanoma treatment.
Glioblastoma Standard of Care: Effects on Tumor Evolution and Reverse Translation in Preclinical Models
Authors: Rodgers Louis T. , Villano John L. , Hartz Anika M. S. , Bauer Björn
Published: July 2024

Glioblastoma (GBM) presents a significant public health challenge as the deadliest and most common malignant brain tumor in adults. Despite standard-of-care treatment, which includes surgery, radiation, and chemotherapy, mortality rates are high, underscoring the critical need for advancing GBM therapy. Over the past two decades, numerous clinical trials have been performed, yet only a small fraction demonstrated a benefit, raising concerns about the predictability of current preclinical models. Traditionally, preclinical studies utilize treatment-naïve tumors, failing to model the clinical scenario where patients undergo standard-of-care treatment prior to recurrence. Recurrent GBM generally exhibits distinct molecular alterations influenced by treatment selection pressures. In this review, we discuss the impact of treatment—surgery, radiation, and chemotherapy—on GBM. We also provide a summary of treatments used in preclinical models, advocating for their integration to enhance the translation of novel strategies to improve therapeutic outcomes in GBM.
Radiosynthesis and Preclinical Evaluation of 18F-Labeled Estradiol Derivatives with Different Lipophilicity for PET Imaging of Breast Cancer
Authors: Friedel Anna , Prante Olaf , Maschauer Simone
Published: July 2024

About 75% of breast tumors show an overexpression of the estradiol receptor (ER), making it a valuable target for tumor diagnosis and therapy. To date, 16α-[18F]fluoroestradiol (FES) is the only FDA-approved imaging probe for the positron emission tomography (PET) imaging of ER-positive (ER+) breast cancer. However, FES has the drawback of a high retention in the liver. Therefore, the aim of this study was the development and preclinical evaluation of estradiol (E2) derivatives with different lipophilicity. Three 18F-labeled prosthetic groups (two glycosyl and one PEG azide) were chosen for conjugation with ethinyl estradiol (EE) by 18F-CuAAC (Cu-catalyzed azide-alkyne cycloaddition). The cellular uptake in ER+ MCF-7 tumor cells was highest for the less hydrophilic derivative (18F-TA-Glyco-EE). In nude mice bearing different breast tumors (ER+ MCF-7 and T47D versus ER− MDA-MB-231), 18F-TA-Glyco-EE revealed a high uptake in the liver (13%ID/g, 30 min p.i.), which decreased over 90 min to 1.2%ID/g, indicating fast hepatobiliary clearance. The statistically significant difference of 18F-TA-Glyco-EE uptake in T47D compared to MDA-MB-231 tumors at 60–90 min p.i. indicated ER-specific uptake, whereas in vivo PET imaging did not provide evidence for specific uptake of 18F-TA-Glyco-EE in MCF-7 tumors, probably due to ER occupation by E2 after E2-dependent MCF-7 tumor growth in mice. However, in vitro autoradiography revealed a high specific binding of 18F-TA-Glyco-EE to ER+ tumor slices. We conclude that 18F-TA-Glyco-EE, with its increased hydrophilicity after deacetylation in the blood and thus rapid washout from non-target tissues, may be a viable alternative to FES for the PET imaging of breast cancer.
Unraveling the Dynamics of Estrogen and Progesterone Signaling in the Endometrium: An Overview
Authors: Dias Da Silva Isabelle , Wuidar Vincent , Zielonka Manon , Pequeux Christel
Published: July 2024

The endometrium is crucial for the perpetuation of human species. It is a complex and dynamic tissue lining the inner wall of the uterus, regulated throughout a woman’s life based on estrogen and progesterone fluctuations. During each menstrual cycle, this multicellular tissue undergoes cyclical changes, including regeneration, differentiation in order to allow egg implantation and embryo development, or shedding of the functional layer in the absence of pregnancy. The biology of the endometrium relies on paracrine interactions between epithelial and stromal cells involving complex signaling pathways that are modulated by the variations of estrogen and progesterone levels across the menstrual cycle. Understanding the complexity of estrogen and progesterone receptor signaling will help elucidate the mechanisms underlying normal reproductive physiology and provide fundamental knowledge contributing to a better understanding of the consequences of hormonal imbalances on gynecological conditions and tumorigenesis. In this narrative review, we delve into the physiology of the endometrium, encompassing the complex signaling pathways of estrogen and progesterone.
Exploring the Influence of Concurrent Nutritional Therapy on Children with Spinal Muscular Atrophy Receiving Nusinersen Treatment
Authors: Pinar Eymen , Ayvaz Bilal Berke , Akkus Erkan , Ulkersoy Ipek , Dilek Tugce Damla , Zindar Yilmaz , Ulug Fitnat , Guzeler Aysel , Kilic Huseyin , Guler Serhat , Beser Omer Faruk , Saltik Sema , Cullu Cokugras Fugen
Published: July 2024

Background This study examines spinal muscular atrophy (SMA), a neuromuscular disease associated with malnutrition. Our goals are to assess how effectively screening tools can detect malnutrition and evaluate the impact of nutritional interventions on neurological outcomes, particularly motor functions. Methods Thirty-seven genetically diagnosed SMA patients (types 1, 2, and 3) under nusinersen therapy were included in the study. The nutritional status of these patients was assessed by using anthropometric measurements, including height for age (HFA), weight for height (WFH), and body mass index (BMI) before and after the study. Additionally, the risk of malnutrition was determined using screening tools, namely the Pediatric Yorkhill Malnutrition Score (PYMS) and the Screening Tool for the Assessment of Malnutrition in Pediatrics (STAMP). Nutritional counseling followed the European Society for Paediatric Gastroenterology Hepatology and Nutrition (ESPGHAN) guidelines and considered the patients’ dietary history, including content and administration method. Motor functions were assessed by validated tests: the Children’s Hospital of Philadelphia Infant Test of Neuromuscular Disorders (CHOP-INTEND) and the Hammersmith Functional Motor Scale—Expanded (HFMSE). Result The study showed an improvement in HFA, by a change from −0.95 to −0.65 (p = 0.015). Conversely, BMI scores decreased from 0.08 to −0.54 (p = 0.015), while WFH and MUAC showed no significant alterations (p = 0.135, p = 0.307). Following nutritional interventions, HFMSE demonstrated a median increase from 29.5 to 30.5 (p = 0.023). Patients identified as being at high risk for malnutrition based on PYMS and STAMP belonged to the moderate-to-severe malnutrition group (BMI Z-score ≤ −2, p = 0.001). Conclusions Use of screening tools in SMA patients is highly beneficial for the early detection of malnutrition. Future research should highlight the importance of combining nutritional management with nusinersen therapy to potentially alter the disease trajectory, especially in motor and neurological functions.
Harnessing the Potential of Helinus integrifolius in Cosmeceutical Research: Toward Sustainable Natural Cosmetics
Authors: Gwanya Hlalanathi Y. , Mbuyiswa Afika-Amazizi N. , Phokwe Ompelege J. , Gxaba Nomagugu , Manganyi Madira C.
Published: July 2024

The growing demand for natural and sustainable ingredients in cosmetic formulations has driven scientists to explore a wide range of botanical resources. Helinus integrifolius, a lesser-known botanical entity, has recently emerged as a formidable contender in this field. This review explores the possibility of incorporating Helinus integrifolius in the cosmetics industry, synthesizing data from the literature on pharmacological, botanical, and cosmetic research. Additionally, this highlights the versatility and multipurpose nature of this botanical resource in cosmetic applications. Furthermore, concerns regarding the safety of contemporary cosmetic products, formulation compatibility, and regulatory aspects are addressed to accelerate its integration into cosmetic products. Ultimately, this review highlights the untapped potential of Helinus integrifolius as a treasured resource for the cosmetics industry, emphasizing its ability to meet the growing consumer demand for eco-friendly, effective, and naturally inspired cosmetic solutions. Through thorough exploration and analysis, this paper aims to stimulate further research and development initiatives aimed at harnessing the cosmetic capabilities of Helinus integrifolius, thus contributing to the advancement of innovation in natural cosmetics.
Directional Acoustic Bulk Waves in a 2D Phononic Crystal
Authors: Deymier Pierre A. , Vasseur Jérôme O. , Runge Keith , Muralidharan Krishna , Khanikaev Alexander , Alù Andrea
Published: July 2024

We used the transfer matrix method to investigate the conditions supporting the existence of directional bulk waves in a two-dimensional (2D) phononic crystal. The 2D crystal was a square lattice of unit cells composed of rectangular subunits constituted of two different isotropic continuous media. We established the conditions on the geometry of the phononic crystal and its constitutive media for the emergence of waves, which, for the same handedness, exhibited a non-zero amplitude in one direction within the crystal’s 2D Brillouin zone and zero amplitude in the opposite direction. Due to time-reversal symmetry, the crystal supported propagation in the reverse direction for the opposite handedness. These features may enable robust directional propagation of bulk acoustic waves and topological acoustic technology.
Prevention of Crystal Agglomeration: Mechanisms, Factors, and Impact of Additives
Authors: Zhang Huixiang , Du Shichao , Wang Yan , Xue Fumin
Published: July 2024

Crystal agglomeration is a common phenomenon for most chemicals and pharmaceuticals. The formation of agglomerates usually lowers product purity and generates a broad particle size distribution. This review focuses on preventing agglomeration in solution crystallization, the storage of crystals, and pharmaceutical preparation processes. The agglomeration mechanisms in these stages are analyzed and the effects of operating parameters are summarized. Furthermore, effective control means related to the crystallization environment are elaborated, including solvents, ultrasound, and additives. Special attention is paid to the influence of additives in preventing the aggregation of both suspensions and dried powders. Besides additives used in solution crystallization, the roles of anti-caking agents, stabilizers of nanosuspensions, and excipients of solid dispersions are also discussed. The additive type and properties like hydrophilicity, hydrophobicity, ionic strength, viscosity, the steric hindrance effect, and intermolecular interactions between additives and crystals can greatly affect the degree of agglomeration.
Genetic Diversity of the Lilac-Crowned Parrot (Amazona finschi), a Species Endemic to Mexico
Authors: Padilla-Jacobo Gabriela , Monterrubio-Rico Tiberio Cesar , Cano-Camacho Horacio , Zavala-Páramo María Guadalupe
Published: July 2024

The Lilac-crowned Amazon (Amazona finschi) is an endemic parrot from western Mexico with a historical distribution in the Pacific Slope from southern Sonora and southwestern Chihuahua to Oaxaca. However, a particularly worrying decline in the extension of its distribution range has been reported in the central and southern regions. Overall, the species is listed in CITES the IUCN Red List of Threatened Species, and the official Mexican standard NOM-059 as endangered. In this study, we aimed to obtain molecular information to support the planning of conservation strategies for A. finschi. For this purpose, we analyzed the genetic diversity and genealogical relationships between two groups of individuals from northern (Sinaloa) and central (Michoacan) portions of the species’ range based on mitochondrial DNA markers. In general agreement with the endangered status of the species, we found low genetic diversity values. However, at the regional level, the northern group showed high genetic diversity and the central group showed a lack of genetic diversity. Furthermore, in agreement with the proposal that A. finschi is monotypic, genealogical relationships revealed a haplotype distributed in the center and the north, although haplotypes exclusive to the north were also found. We suggest a differentiated management of northern and central populations to preserve evolutionary potential.
First Records of Feather Mites and Haemosporidian Parasites in the Isabelline Wheatear (Oenanthe isabellina) from the Westernmost Part of the Species Breeding Range
Authors: Kolarova Nevena , Bobeva Aneliya , Ilieva Mihaela , Sjöholm Christoffer , Dimitrov Dimitar
Published: July 2024

Host range expansions are an important factor for shaping the community of associated symbiotic organisms. Birds, as a highly mobile group of animals, are of particular interest to study with respect to the diversity and the distribution of the organisms using them as hosts during such large-scale movements. The Isabelline wheatear (Oenanthe isabellina) is a species with a main breeding area in Asia, which has expanded west, towards the Balkans, since the middle of the last century. We collected feather mites and blood samples for haemosporidian parasites screening from adult and juvenile Isabelline wheatears from the westernmost edge of the species breeding area in western Bulgaria. The feather mite species Alaudicola rosickyi (Černy, 1963), previously found on other wheatear species, was found for the first time on Isabelline wheatears. One species of avian malaria parasites—Plasmodium relictum (Grassi and Feletti, 1891) (lineage SGS1)—represented the first record of a malaria parasite in this host species in Europe. Increasing the sampling in the South European populations of the species will shed light on blood parasite species diversity and will reveal if other feather mite species have followed their host during its breeding range expansion.
Fish of Low Commercial Value in Lakes of Different Trophic Status (Poland)
Authors: Kalinowska Krystyna , Ulikowski Dariusz , Kozłowski Michał , Traczuk Piotr , Szkudlarek Maciej , Stawecki Konrad , Kapusta Andrzej
Published: July 2024

In a commercial fishery, some fish are classified as low-value, but their classification varies in different countries. The aim of this study was to determine the abundance, contribution, and dominance of low-value fish species, such as Abramis brama < 1000 g, Alburnus alburnus, Blicca bjoerkna, Gymnocephalus cernua, Perca fluviatilis < 100 g, Rutilus rutilus < 200 g, and Scardinius erythrophthalmus < 200 g, in 145 Polish lakes of different areas, depths, and trophic statuses situated in the northern and central parts of Poland in 2021. Perca fluviatilis and R. rutilus were the most frequent low-value species (100% and 99%, respectively). The contribution of all low-value fish to the total biomass of caught fish was relatively high, ranging from 37% in the mesotrophic lake to 100% in the eutrophic lake (mean of 77 ± 14%). Lakes in which the contribution of low-value species exceeded 90% were relatively numerous (24 lakes, 17% of the studied lakes). Among a total of about 437.5 thousand low-value fish, 261 thousand specimens (60%) had a body weight of below 10 g. All low-value fish species, except for P. fluviatilis and S. erythrophthalmus, were related to the studied environmental variables. The relative biomass of these species increased with increasing lake productivity, while it decreased with the increasing maximum and mean depth of the studied lakes. The high contribution of low-value fish to the total biomass in many lakes indicates the need for the constant monitoring of the abundance and structure of fish communities and the use of appropriate actions (biomanipulation and stocking with piscivorous fish species) to improve the ecological condition of lakes.
BELMASK—An Audiovisual Dataset of Adversely Produced Speech for Auditory Cognition Research
Authors: Moshona Cleopatra Christina , Rudawski Frederic , Fiebig André , Sarradj Ennes
Published: July 2024

In this article, we introduce the Berlin Dataset of Lombard and Masked Speech (BELMASK), a phonetically controlled audiovisual dataset of speech produced in adverse speaking conditions, and describe the development of the related speech task. The dataset contains in total 128 min of audio and video recordings of 10 German native speakers (4 female, 6 male) with a mean age of 30.2 years (SD: 6.3 years), uttering matrix sentences in cued, uninstructed speech in four conditions: (i) with a Filtering Facepiece P2 (FFP2) mask in silence, (ii) without an FFP2 mask in silence, (iii) with an FFP2 mask while exposed to noise, (iv) without an FFP2 mask while exposed to noise. Noise consisted of mixed-gender six-talker babble played over headphones to the speakers, triggering the Lombard effect. All conditions are readily available in face-and-voice and voice-only formats. The speech material is annotated, employing a multi-layer architecture, and was originally conceptualized to be used for the administration of a working memory task. The dataset is stored in a restricted-access Zenodo repository and is available for academic research in the area of speech communication, acoustics, psychology and related disciplines upon request, after signing an End User License Agreement (EULA).
Performance of Intraoperative Contrast-Enhanced Ultrasound (Io-CEUS) in the Diagnosis of Primary Lung Cancer
Authors: Schauer Martin Ignaz , Jung Ernst Michael , Hofmann Hans-Stefan , Platz Batista da Silva Natascha , Akers Michael , Ried Michael
Published: July 2024

Background: Suspicious tumors of the lung require specific staging, intraoperative detection, and histological confirmation. We performed an intrathoracic, intraoperative contrast-enhanced ultrasound (Io-CEUS) for characterization of lung cancer. Methods: Retrospective analysis of prospectively collected data on the application of Io-CEUS in thoracic surgery for patients with operable lung cancer. Analysis of the preoperative chest CT scan and FDG-PET/CT findings regarding criteria of malignancy. Immediately before lung resection, the intrathoracic Io-CEUS was performed with a contrast-enabled T-probe (6–9 MHz—L3-9i-D) on a high-performance ultrasound machine (Loqic E9, GE). In addition to intraoperative B-mode, color-coded Doppler sonography (CCDS), or power Doppler (macrovascularization) of the lung tumor, contrast enhancement (Io-CEUS) was used after venous application of 2.4–5 mL sulfur hexafluoride (SonoVue, Bracco, Italy) for dynamic recording of microvascularization. The primary endpoint was the characterization of operable lung cancer with Io-CEUS. Secondly, the results of Io-CEUS were compared with the preoperative staging. Results: The study included 18 patients with operable lung cancer, who received Io-CEUS during minimally invasive thoracic surgery immediately prior to lung resection. In the chest CT scan, the mean size of the lung tumors was 2.54 cm (extension of 0.7–4.5 cm). The mean SUV in the FDG-PET/CT was 7.6 (1.2–16.9). All lung cancers were detected using B-mode and power Doppler confirmed macrovascularization (100%) of the tumors. In addition, Io-CEUS showed an early wash-in with marginal and mostly simultaneous central contrast enhancement. Conclusions: The intrathoracic application of Io-CEUS demonstrated a peripheral and simultaneous central contrast enhancement in the early phase, which seems to be characteristic of lung cancer. In comparison to preoperative imaging, Io-CEUS was on par with the detection of malignancy and offers an additional tool for the intraoperative assessment of lung cancer before resection.
Contextual Enhancement–Interaction and Multi-Scale Weighted Fusion Network for Aerial Tracking
Authors: Wang Bo , Wang Xuan , Ma Linglong , Zuo Yujia , Liu Chenglong
Published: July 2024

Siamese-based trackers have been widely utilized in UAV visual tracking due to their outstanding performance. However, UAV visual tracking encounters numerous challenges, such as similar targets, scale variations, and background clutter. Existing Siamese trackers face two significant issues: firstly, they rely on single-branch features, limiting their ability to achieve long-term and accurate aerial tracking. Secondly, current tracking algorithms treat multi-level similarity responses equally, making it difficult to ensure tracking accuracy in complex airborne environments. To tackle these challenges, we propose a novel UAV tracking Siamese network named the contextual enhancement–interaction and multi-scale weighted fusion network, which is designed to improve aerial tracking performance. Firstly, we designed a contextual enhancement–interaction module to improve feature representation. This module effectively facilitates the interaction between the template and search branches and strengthens the features of each branch in parallel. Specifically, a cross-attention mechanism within the module integrates the branch information effectively. The parallel Transformer-based enhancement structure improves the feature saliency significantly. Additionally, we designed an efficient multi-scale weighted fusion module that adaptively weights the correlation response maps across different feature scales. This module fully utilizes the global similarity response between the template and the search area, enhancing feature distinctiveness and improving tracking results. We conducted experiments using several state-of-the-art trackers on aerial tracking benchmarks, including DTB70, UAV123, UAV20L, and UAV123@10fps, to validate the efficacy of the proposed network. The experimental results demonstrate that our tracker performs effectively in complex aerial tracking scenarios and competes well with state-of-the-art trackers.
Entropy-Based Strategies for Multi-Bracket Pools
Authors: Brill Ryan S. , Wyner Abraham J. , Barnett Ian J.
Published: July 2024

Much work in the parimutuel betting literature has discussed estimating event outcome probabilities or developing optimal wagering strategies, particularly for horse race betting. Some betting pools, however, involve betting not just on a single event, but on a tuple of events. For example, pick six betting in horse racing, March Madness bracket challenges, and predicting a randomly drawn bitstring each involve making a series of individual forecasts. Although traditional optimal wagering strategies work well when the size of the tuple is very small (e.g., betting on the winner of a horse race), they are intractable for more general betting pools in higher dimensions (e.g., March Madness bracket challenges). Hence we pose the multi-brackets problem: supposing we wish to predict a tuple of events and that we know the true probabilities of each potential outcome of each event, what is the best way to tractably generate a set of n predicted tuples? The most general version of this problem is extremely difficult, so we begin with a simpler setting. In particular, we generate n independent predicted tuples according to a distribution having optimal entropy. This entropy-based approach is tractable, scalable, and performs well.
A Field-Theory Approach for Modeling Dissipative Relativistic Fluids
Authors: Andersson Nils , Celora Thomas , Comer Gregory , Hawke Ian
Published: July 2024

We develop an action principle for producing a single-fluid two-constituent system with dissipation in general relativity. The two constituents in the model are particles and entropy. The particle flux creation rate is taken to be zero, while the entropy creation rate is non-zero. Building on previous work, it is demonstrated that a new term (the proper time derivative of the matter space “metric”) is required in the Lagrangian in order to produce terms typically associated with bulk and shear viscosity. Equations of motion, entropy creation rate, and energy–momentum–stress tensor are derived. Using an Onsager approach of identifying thermodynamic “forces” and “fluxes”, a model is produced which delivers the same entropy creation rate as the standard, relativistic Navier–Stokes equations. This result is then contrasted with a model generated in the spirit of the action principle, which takes as its starting point a specific Lagrangian and then produces the equations of motion, entropy creation rate, and energy–momentum–stress tensor. Unlike the equations derived from Onsager reasoning, where the analogs of the bulk and shear viscosity coefficients are prescribed “externally”, we find that the forms of the coefficients in the second example are a direct result of the specified Lagrangian. Furthermore, the coefficients are shown to satisfy evolution equations along the fluid worldline, also a product of the specific Lagrangian.