bluuebunny/crossref_metadata_2025 huggingface dataset:
Improving Sexual Health Education Programs for Adolescent Students through Game-Based Learning and Gamification
Authors: Haruna Hussein , Hu Xiao , Chu Samuel Kai Wah , Mellecker Robin R. , Gabriel Goodluck , Ndekao Patrick Siril
Published: September 2018

An effective innovative pedagogy for sexual health education is required to meet the demands of technology savvy digital natives. This study investigates the extent to which game-based learning (GBL) and gamification could improve the sexual health education of adolescent students. We conducted a randomized control trial of GBL and gamification experimental conditions. We made a comparison with traditional teaching as a control condition in order to establish differences between the three teaching conditions. The sexual health education topics were delivered in a masked fashion, 40-min a week for five weeks. A mixed-method research approach was uses to assess and analyze the results for 120 students from a secondary school in Dar Es Salaam, Tanzania. Students were divided into groups of 40 for each of the three teaching methods: GBL, gamification, and the control group (the traditional teaching method). The average post-test scores for GBL (Mean = 79.94, SD = 11.169) and gamification (Mean = 79.23, SD = 9.186) were significantly higher than the control group Mean = 51.93, SD = 18.705 (F (2, 117) = 54.75, p = 0.001). Overall, statistically significant differences (p ≤ 0.05) were found for the constructs of Motivation, Attitude, Knowledge, and Engagement (MAKE). This study suggests that the two innovative teaching approaches can be used to improve the sexual health education of adolescent students. The methods can potentially contribute socially, particularly in improving sexual health behaviour and adolescents’ knowledge in regions plagued by years of sexual health problems, including HIV/AIDS.
Associations between Bystanders and Perpetrators of Online Hate: The Moderating Role of Toxic Online Disinhibition
Authors: Wachs Sebastian , Wright Michelle F.
Published: September 2018

Hatred directed at members of groups due to their origin, race, gender, religion, or sexual orientation is not new, but it has taken on a new dimension in the online world. To date, very little is known about online hate among adolescents. It is also unknown how online disinhibition might influence the association between being bystanders and being perpetrators of online hate. Thus, the present study focused on examining the associations among being bystanders of online hate, being perpetrators of online hate, and the moderating role of toxic online disinhibition in the relationship between being bystanders and perpetrators of online hate. In total, 1480 students aged between 12 and 17 years old were included in this study. Results revealed positive associations between being online hate bystanders and perpetrators, regardless of whether adolescents had or had not been victims of online hate themselves. The results also showed an association between toxic online disinhibition and online hate perpetration. Further, toxic online disinhibition moderated the relationship between being bystanders of online hate and being perpetrators of online hate. Implications for prevention programs and future research are discussed.
Proteomics and Lipidomics in Inflammatory Bowel Disease Research: From Mechanistic Insights to Biomarker Identification
Authors: Titz Bjoern , Gadaleta Raffaella M. , Lo Sasso Giuseppe , Elamin Ashraf , Ekroos Kim , Ivanov Nikolai V. , Peitsch Manuel C. , Hoeng Julia
Published: September 2018

Inflammatory bowel disease (IBD) represents a group of progressive disorders characterized by recurrent chronic inflammation of the gut. Ulcerative colitis and Crohn′s disease are the major manifestations of IBD. While our understanding of IBD has progressed in recent years, its etiology is far from being fully understood, resulting in suboptimal treatment options. Complementing other biological endpoints, bioanalytical “omics” methods that quantify many biomolecules simultaneously have great potential in the dissection of the complex pathogenesis of IBD. In this review, we focus on the rapidly evolving proteomics and lipidomics technologies and their broad applicability to IBD studies; these range from investigations of immune-regulatory mechanisms and biomarker discovery to studies dissecting host–microbiome interactions and the role of intestinal epithelial cells. Future studies can leverage recent advances, including improved analytical methodologies, additional relevant sample types, and integrative multi-omics analyses. Proteomics and lipidomics could effectively accelerate the development of novel targeted treatments and the discovery of complementary biomarkers, enabling continuous monitoring of the treatment response of individual patients; this may allow further refinement of treatment and, ultimately, facilitate a personalized medicine approach to IBD.
Modulation of Insulin Resistance and the Adipocyte-Skeletal Muscle Cell Cross-Talk by LCn-3PUFA
Authors: Pinel Alexandre , Rigaudière Jean-Paul , Jouve Chrystèle , Capel Frédéric
Published: September 2018

The cross-talk between skeletal muscle and adipose tissue is involved in the development of insulin resistance (IR) in skeletal muscle, leading to the decrease in the anabolic effect of insulin. We investigated if the long chain polyunsaturated n-3 fatty acids (LCn-3PUFA), eicosapentaenoic and docosapentaenoic acids (EPA and DPA, respectively) could (1) regulate the development of IR in 3T3-L1 adipocytes and C2C12 muscle cells and (2) inhibit IR in muscle cells exposed to conditioned media (CM) from insulin-resistant adipocytes. Chronic insulin (CI) treatment of adipocytes and palmitic acid (PAL) exposure of myotubes were used to induce IR in the presence, or not, of LCn-3PUFA. EPA (50 µM) and DPA (10 µM) improved PAL-induced IR in myotubes, but had only a partial effect in adipocytes. CM from adipocytes exposed to CI induced IR in C2C12 myotubes. Although DPA increased the mRNA levels of genes involved in fatty acid (FA) beta-oxidation and insulin signaling in adipocytes, it was not sufficient to reduce the secretion of inflammatory cytokines and prevent the induction of IR in myotubes exposed to adipocyte’s CM. Treatment with DPA was able to increase the release of adiponectin by adipocytes into CM. In conclusion, DPA is able to protect myotubes from PAL-induced IR, but not from IR induced by CM from adipocytes.
A Dairy-Derived Ghrelinergic Hydrolysate Modulates Food Intake In Vivo
Authors: Howick Ken , Wallace-Fitzsimons Shauna E. , Kandil Dalia , Chruścicka Barbara , Calis Mert , Murphy Eoin , Murray Brian A. , Fernandez Ayoa , Barry Kate M. , Kelly Phil M. , Ryan Aoife M. , Cryan John F. , Griffin Brendan T. , Schellekens Harriët
Published: September 2018

Recent times have seen an increasing move towards harnessing the health-promoting benefits of food and dietary constituents while providing scientific evidence to substantiate their claims. In particular, the potential for bioactive protein hydrolysates and peptides to enhance health in conjunction with conventional pharmaceutical therapy is being investigated. Dairy-derived proteins have been shown to contain bioactive peptide sequences with various purported health benefits, with effects ranging from the digestive system to cardiovascular circulation, the immune system and the central nervous system. Interestingly, the ability of dairy proteins to modulate metabolism and appetite has recently been reported. The ghrelin receptor (GHSR-1a) is a G-protein coupled receptor which plays a key role in the regulation of food intake. Pharmacological manipulation of the growth hormone secretagogue receptor-type 1a (GHSR-1a) receptor has therefore received a lot of attention as a strategy to combat disorders of appetite and body weight, including age-related malnutrition and the progressive muscle wasting syndrome known as cachexia. In this study, a milk protein-derivative is shown to increase GHSR-1a-mediated intracellular calcium signalling in a concentration-dependent manner in vitro. Significant increases in calcium mobilisation were also observed in a cultured neuronal cell line heterologously expressing the GHS-R1a. In addition, both additive and synergistic effects were observed following co-exposure of GHSR-1a to both the hydrolysate and ghrelin. Subsequent in vivo studies monitored standard chow intake in healthy male and female Sprague-Dawley rats after dosing with the casein hydrolysate (CasHyd). Furthermore, the provision of gastro-protected oral delivery to the bioactive in vivo may aid in the progression of in vitro efficacy to in vivo functionality. In summary, this study reports a ghrelin-stimulating bioactive peptide mixture (CasHyd) with potent effects in vitro. It also provides novel and valuable translational data supporting the potential role of CasHyd as an appetite-enhancing bioactive. Further mechanistic studies are required in order to confirm efficacy as a ghrelinergic bioactive in susceptible population groups.
CoII(Chromomycin)2 Complex Induces a Conformational Change of CCG Repeats from i-Motif to Base-Extruded DNA Duplex
Authors: Chen Yu-Wen , Satange Roshan , Wu Pei-Ching , Jhan Cyong-Ru , Chang Chung-ke , Chung Kuang-Ren , Waring Michael J. , Lin Sheng-Wei , Hsieh Li-Ching , Hou Ming-Hon
Published: September 2018

We have reported the propensity of a DNA sequence containing CCG repeats to form a stable i-motif tetraplex structure in the absence of ligands. Here we show that an i-motif DNA sequence may transition to a base-extruded duplex structure with a GGCC tetranucleotide tract when bound to the (CoII)-mediated dimer of chromomycin A3, CoII(Chro)2. Biophysical experiments reveal that CCG trinucleotide repeats provide favorable binding sites for CoII(Chro)2. In addition, water hydration and divalent metal ion (CoII) interactions also play a crucial role in the stabilization of CCG trinucleotide repeats (TNRs). Our data furnish useful structural information for the design of novel therapeutic strategies to treat neurological diseases caused by repeat expansions.
Selenite Reduction and the Biogenesis of Selenium Nanoparticles by Alcaligenes faecalis Se03 Isolated from the Gut of Monochamus alternatus (Coleoptera: Cerambycidae)
Authors: Wang Yuting , Shu Xian , Zhou Qing , Fan Tao , Wang Taichu , Chen Xue , Li Minghao , Ma Yuhan , Ni Jun , Hou Jinyan , Zhao Weiwei , Li Ruixue , Huang Shengwei , Wu Lifang
Published: September 2018

In this study, a bacterial strain exhibiting high selenite (Na2SeO3) tolerance and reduction capacity was isolated from the gut of Monochamus alternatus larvae and identified as Alcaligenes faecalis Se03. The isolate exhibited extreme tolerance to selenite (up to 120 mM) when grown aerobically. In the liquid culture medium, it was capable of reducing nearly 100% of 1.0 and 5.0 mM Na2SeO3 within 24 and 42 h, respectively, leading to the formation of selenium nanoparticles (SeNPs). Electron microscopy and energy dispersive X-ray analysis demonstrated that A. faecalis Se03 produced spherical electron-dense SeNPs with an average hydrodynamic diameter of 273.8 ± 16.9 nm, localized mainly in the extracellular space. In vitro selenite reduction activity and real-time PCR indicated that proteins such as sulfite reductase and thioredoxin reductase present in the cytoplasm were likely to be involved in selenite reduction and the SeNPs synthesis process in the presence of NADPH or NADH as electron donors. Finally, using Fourier transform infrared spectroscopy, protein and carbohydrate residues were detected on the surface of the biogenic SeNPs. Based on these observations, A. faecalis Se03 has the potential to be an eco-friendly candidate for the bioremediation of selenium-contaminated soil/water and a bacterial catalyst for the biogenesis of SeNPs.
Analysis of bZIP Transcription Factor Family and Their Expressions under Salt Stress in Chlamydomonas reinhardtii
Authors: Ji Chunli , Mao Xue , Hao Jingyun , Wang Xiaodan , Xue Jinai , Cui Hongli , Li Runzhi
Published: September 2018

The basic leucine-region zipper (bZIP) transcription factors (TFs) act as crucial regulators in various biological processes and stress responses in plants. Currently, bZIP family members and their functions remain elusive in the green unicellular algae Chlamydomonas reinhardtii, an important model organism for molecular investigation with genetic engineering aimed at increasing lipid yields for better biodiesel production. In this study, a total of 17 C. reinhardtii bZIP (CrebZIP) TFs containing typical bZIP structure were identified by a genome-wide analysis. Analysis of the CrebZIP protein physicochemical properties, phylogenetic tree, conserved domain, and secondary structure were conducted. CrebZIP gene structures and their chromosomal assignment were also analyzed. Physiological and photosynthetic characteristics of C. reinhardtii under salt stress were exhibited as lower cell growth and weaker photosynthesis, but increased lipid accumulation. Meanwhile, the expression profiles of six CrebZIP genes were induced to change significantly during salt stress, indicating that certain CrebZIPs may play important roles in mediating photosynthesis and lipid accumulation of microalgae in response to stresses. The present work provided a valuable foundation for functional dissection of CrebZIPs, benefiting the development of better strategies to engineer the regulatory network in microalgae for enhancing biofuel and biomass production.
Specialized Living Wound Dressing Based on the Self-Assembly Approach of Tissue Engineering
Authors: Cantin-Warren Laurence , Guignard Rina , Cortez Ghio Sergio , Larouche Danielle , Auger François A. , Germain Lucie
Published: September 2018

There is a high incidence of failure and recurrence for chronic skin wounds following conventional therapies. To promote healing, the use of skin substitutes containing living cells as wound dressings has been proposed. The aim of this study was to produce a scaffold-free cell-based bilayered tissue-engineered skin substitute (TES) containing living fibroblasts and keratinocytes suitable for use as wound dressing, while considering production time, handling effort during the manufacturing process, and stability of the final product. The self-assembly method, which relies on the ability of mesenchymal cells to secrete and organize connective tissue sheet sustaining keratinocyte growth, was used to produce TESs. Three fibroblast-seeding densities were tested to produce tissue sheets. At day 17, keratinocytes were added onto 1 or 3 (reference method) stacked tissue sheets. Four days later, TESs were subjected either to 4, 10, or 17 days of culture at the air–liquid interface (A/L). All resulting TESs were comparable in terms of their histological aspect, protein expression profile and contractile behavior in vitro. However, signs of extracellular matrix (ECM) digestion that progressed over culture time were noted in TESs produced with only one fibroblast-derived tissue sheet. With lower fibroblast density, the ECM of TESs was almost completely digested after 10 days A/L and lost histological integrity after grafting in athymic mice. Increasing the fibroblast seeding density 5 to 10 times solved this problem. We conclude that the proposed method allows for a 25-day production of a living TES, which retains its histological characteristics in vitro for at least two weeks.
Effects of a Si-doped InGaN Underlayer on the Optical Properties of InGaN/GaN Quantum Well Structures with Different Numbers of Quantum Wells
Authors: Christian George , Kappers Menno , Massabuau Fabien , Humphreys Colin , Oliver Rachel , Dawson Philip
Published: September 2018

In this paper we report on the optical properties of a series of InGaN polar quantum well structures where the number of wells was 1, 3, 5, 7, 10 and 15 and which were grown with the inclusion of an InGaN Si-doped underlayer. When the number of quantum wells is low then the room temperature internal quantum efficiency can be dominated by thermionic emission from the wells. This can occur because the radiative recombination rate in InGaN polar quantum wells can be low due to the built-in electric field across the quantum well which allows the thermionic emission process to compete effectively at room temperature limiting the internal quantum efficiency. In the structures that we discuss here, the radiative recombination rate is increased due to the effects of the Si-doped underlayer which reduces the electric field across the quantum wells. This results in the effect of thermionic emission being largely eliminated to such an extent that the internal quantum efficiency at room temperature is independent of the number of quantum wells.
Effect of Ti Transition Layer Thickness on the Structure, Mechanical and Adhesion Properties of Ti-DLC Coatings on Aluminum Alloys
Authors: Cao Hongshuai , Qi Fugang , Ouyang Xiaoping , Zhao Nie , Zhou Yun , Li Beibei , Luo Wenzhong , Liao Bin , Luo Jun
Published: September 2018

Multilayers of Ti doped diamond-like carbon (Ti-DLC) coatings were deposited on aluminum alloys by filtered cathodic vacuum arc (FCVA) technology using C2H2 as a reactive gas. The effect of different Ti transition layer thicknesses on the structure, mechanical and adhesion properties of the coatings, was investigated by scanning electron microscopy (SEM), Raman spectroscopy, X-ray photoelectron spectroscopy (XPS), nanoindentation and a scratch tester. The results showed that the Ti transition layer could improve interfacial transition between the coating and the substrate, which was beneficial in obtaining excellent adhesion of the coatings. The Ti transition layer thickness had no significant influence on the composition and structure of the coatings, whereas it affected the distortion of the sp2-C bond angle and length. Nanoindentation and scratch test results indicated that the mechanical and adhesion properties of the Ti-DLC coatings depended on the Ti transition layer thickness. The Ti transition layer proved favorable in decreasing the residual compressive stress of the coating. As the Ti transition layer thickness increased, the hardness value of the coating gradually decreased. However, its elastic modulus and adhesion exhibited an initial decrease followed by an increasing fluctuation. Among them, the Ti-DLC coating with a Ti transition layer thickness of 1.1 μm exhibited superior mechanical properties.
Developing a New Generation of Therapeutic Dental Polymers to Inhibit Oral Biofilms and Protect Teeth
Authors: Zhang Ke , Baras Bashayer , Lynch Christopher , Weir Michael , Melo Mary , Li Yuncong , Reynolds Mark , Bai Yuxing , Wang Lin , Wang Suping , Xu Hockin
Published: September 2018

Polymeric tooth-colored restorations are increasingly popular in dentistry. However, restoration failures remain a major challenge, and more than 50% of all operative work was devoted to removing and replacing the failed restorations. This is a heavy burden, with the expense for restoring dental cavities in the U.S. exceeding $46 billion annually. In addition, the need is increasing dramatically as the population ages with increasing tooth retention in seniors. Traditional materials for cavity restorations are usually bioinert and replace the decayed tooth volumes. This article reviews cutting-edge research on the synthesis and evaluation of a new generation of bioactive dental polymers that not only restore the decayed tooth structures, but also have therapeutic functions. These materials include polymeric composites and bonding agents for tooth cavity restorations that inhibit saliva-based microcosm biofilms, bioactive resins for tooth root caries treatments, polymers that can suppress periodontal pathogens, and root canal sealers that can kill endodontic biofilms. These novel compositions substantially inhibit biofilm growth, greatly reduce acid production and polysaccharide synthesis of biofilms, and reduce biofilm colony-forming units by three to four orders of magnitude. This new class of bioactive and therapeutic polymeric materials is promising to inhibit tooth decay, suppress recurrent caries, control oral biofilms and acid production, protect the periodontium, and heal endodontic infections.
Effects of V and Co Element Addition on Microstructures and the Mechanical Properties of In Situ Biphasic Hybrid (TiCxNy–TiB2)/Ni Cermets
Authors: Qiu Feng , Duan Xiangzheng , Li Xiujuan , Yang Hongyu , Wang Yawei
Published: September 2018

In situ micro-(TiCxNy–TiB2)/Ni cermets with different Co and V content (2,5 and 8 wt.%) were successfully fabricated by combustion synthesis and hot press consolidation in Ni–(V/Co)–Ti–B4C–BN systems. The results indicate that as Co content increased from 0 to 8 wt.%, the average sizes of the ceramic particles decreased, when the content of V increased from 0 to 8 wt.%, the size of the ceramic particles first decreased and then increased, and when the V content is 5%, the ceramic particle size is the smallest. The Co element did not participate in the SHS reaction and was a diluent; therefore, when the Co element was added, the combustion temperature continued to decrease. When the V content was no more than 5 wt.%, as the V content increased, the maximum combustion temperature decreased. When the content of V was less than 5 wt.%, the concentration of V was not sufficient to greatly promote the generation of VN. Therefore, V absorbed a large amount of heat during the reaction, resulting in a continuous decrease in the reaction temperature of the reaction system during the reaction. When the content of the added V continued to increase to 8 wt.%, V participated in the reaction, which was exothermic. The results indicate that as Co content increased from 0 to 8 wt.%, the average sizes of the ceramic particles decreased, and the cermets with 5 wt.% Co possessed the best comprehensive properties: the highest hardness (1967 Hv), superior compression strength (3.25 GPa) and higher fracture strain (3.3%). Correspondingly, when the V content was 8 wt.%, the ultimate compressive strength and hardness of the cermets reached 1823 Hv and 3.11 GPa, respectively, 262 Hv and 0.17 GPa higher than those of the unalloyed cermets, respectively. Furthermore, the effects of Co and V on strengthening mechanisms were analyzed.
Study on Surface Roughness of Gcr15 Machined by Micro-Texture PCBN Tools
Authors: Pan Chen , Li Qinghua , Hu Kaixing , Jiao Yuxin , Song Yumei
Published: September 2018

This paper applies micro textures to the rake face of PCBN (Polycrystalline Cubic Boron Nitride) tools, including three types of micro textures that are microgroove textures vertical to the cutting edge, microgroove textures parallel to the cutting edge, and microhole textures. In this paper, the effects of different cutting speeds on the surface quality of hardened bearing steel GCr15 by dry turning with non-texture PCBN tools and micro-texture PCBN tools are studied, and the surface roughness values obtained by different micro textures were compared and analyzed. The results showed that, compared to that of non-texture tools, the influence degree of the micro-texture tools on the machined surface roughness was different. The microhole texture and vertical microgroove texture were able to effectively reduce the surface roughness of the workpiece, and microhole texture had the best effective influence on surface roughness, but the parallel microgroove texture increased surface roughness. The influence of cutting speeds on surface roughness was different due to different types of micro textures. The influence of micro textures on surface roughness has huge potential for tool applications.
Petrology of Peridotites and Nd-Sr Isotopic Composition of Their Clinopyroxenes from the Middle Andaman Ophiolite, India
Authors: Morishita Tomoaki , Yoshikawa Masako , Tamura Akihiro , Guotana Juan Miguel , Ghosh Biswajit
Published: September 2018

The Andaman Ophiolite, India, is located at the southeastern end of the Tethyan ophiolites. We examine petrology and mineralogy of two lherzolites and a completely serpentinized dunite associated with lherzolite from the middle Andaman Island. Major and trace element compositions of minerals in the lherzolites suggest their residual origin after low-degree of partial melting with less flux infiltration, and are similar to those of abyssal peridotites recovered from mid-ocean ridges. The dunite with spinels having low-Cr/(Cr + Al) ratio was formed by interaction between peridotite and mid-ocean ridge basalt-like melt. The 87Sr/86Sr and 143Nd/144Nd isotopic systematics of clinopyroxenes of the two lherzolites are consistent with MORB-type mantle source. Petrology and light rare earth element (LREE)-depleted patterns of clinopyroxene from the studied lhezolites are the same as those from some of the western Tethyan ophiolites. The age-corrected initial εNd values of the Tethyan lherzolite clinopyroxenes with LREE-depleted patterns are likely to be consistent with the depleted mantle evolution line.
Analysis of Contact Traces of Cannabis by In-Tube Solid-Phase Microextraction Coupled to Nanoliquid Chromatography
Authors: Jornet-Martínez Neus , Ortega-Sierra Adrián , Verdú-Andrés Jorge , Herráez-Hernández Rosa , Campíns-Falcó Pilar
Published: September 2018

Because of its inherent qualities, in-tube solid-phase microextraction (IT-SPME) coupled on-line to nanoliquid chromatography (nanoLC) can be a very powerful tool to address the new challenges of analytical laboratories such as the analysis of traces of complex samples. This is the case of the detection of contact traces of drugs, especially cannabis. The main difficulties encountered in the analysis of traces of cannabis plants on surfaces are the low amount of sample available (typically < 1 mg), the complexity of the matrix, and the low percentages of cannabinoic compounds in the samples. In this work, a procedure is described for the detection of residues of cannabis on different surfaces based on the responses obtained by IT-SPME coupled to nanoLC with UV diode array detection (DAD) for the cannabinoids Δ9-tetrahydrocannabinol (THC), cannabidiol (CBD), and cannabinol (CBN); the proposed conditions can also be applied for quantitative purposes through the measurement of the percentage of THC, the most abundant cannabinoid in plants. The method is based on collecting the suspected drug samples with cotton swabs, followed by the extraction of the target compounds by ultrasound assisted extraction. The extracts are then separated and processed by IT-SPME-nanoLC. The proposed approach has been applied to the detection of traces of cannabis in different kind of items (plastic bags, office paper, aluminum foil, cotton cloths, and hand skin). Sample amounts as low as 0.08 mg have been collected and analysed for THC. The selectivity and effect of the storage conditions on the levels of THC have also been evaluated. The percentages of THC in the samples typically ranged from 0.6% to 2.8%, which means that amounts of this compound as low as 1–2 µg were adequately detected and quantified. For the first time, the reliability of IT-SPME-nanoLC for the analysis of complex matrices such as cannabis plant extracts has been demonstrated.
Microwave (MW), Ultrasound (US) and Combined Synergic MW-US Strategies for Rapid Functionalization of Pharmaceutical Use Phenols
Authors: Pawełczyk Anna , Sowa-Kasprzak Katarzyna , Olender Dorota , Zaprutko Lucjusz
Published: September 2018

Increasingly stringent regulations aimed at protection of the natural environment have stimulated the search for new synthetic methodologies in organic and medicinal chemistry having no or minimum harmful effect. An interesting approach is the use of alternative activation factors, microwaves (MW) or ultrasounds (US) and also their cross-combination, which has been tested in the fast and efficient creation of new structures. At present, an easy and green hybrid strategy (“Lego” chemistry) is generally recommended for the design of new substances from different chemistry building blocks. Often, selected biologically active components with specific chemical reactivities are integrated by a suitably designed homo- or heterodifunctional linker that modifies the functionality of the starting structure, allowing easy covalent linkage to another molecule. In this study, a fast introduction of heterodifunctional halogenoacidic linker to selected mono-, di- and triphenolic active substances, allowing their functionalization, was investigated. Nucleophilic substitution reaction was chosen to produce final ethers with the reactive carboxylic group from phenols. The functionalization was performed using various green factors initiating and supporting the chemical reactions (MW, US, MW-US). The benefits of the three green supporting methods and different conditions of reactions were analyzed and compared with the results of the reaction performed by conventional methods.
Optimization of Aminoimidazole Derivatives as Src Family Kinase Inhibitors
Authors: Francini Cinzia Maria , Musumeci Francesca , Fallacara Anna Lucia , Botta Lorenzo , Molinari Alessio , Artusi Roberto , Mennuni Laura , Angelucci Adriano , Schenone Silvia
Published: September 2018

Protein kinases have emerged as crucial targets for cancer therapy over the last decades. Since 2001, 40 and 39 kinase inhibitors have been approved by FDA and EMA, respectively, and the majority are antineoplastic drugs. Morevoer, many candidates are currently in clinical trials. We previously reported a small library of 4-aminoimidazole and 2-aminothiazole derivatives active as Src family kinase (SFK) inhibitors. Starting from these results, we decided to perform an optimization study applying a mix and match strategy to identify a more potent generation of 4-aminoimidazoles. Firstly, a computational study has been performed, then compounds showing the best predicted docking scores were synthesized and screened in a cell-free assay for their SFK inhibitory activity. All the new chemical entities showed IC50s in the nanomolar range, with 2–130 fold increased activities compared to the previously reported inhibitors. Finally, the most active compounds have been tested on three cancer cell lines characterized by Src hyperactivation. Compounds 4k and 4l showed an interesting antiproliferative activity on SH-SY5Y neuroblastoma (NB) cell line. In this assay, the compounds resulted more potent than dasatinib, a tyrosine kinase inhibitor approved for the treatment of leukemias and in clinical trials for NB.
Synthesis and Antisense Properties of 2′β-F-Arabinouridine Modified Oligonucleotides with 4′-C-OMe Substituent
Authors: He Xiao-Yang , Wang Jing , Lu Dan-Dan , Wang Sheng-Qi
Published: September 2018

A novel 2′-F,4′-C-OMe–arabinouridine (araU) was successfully synthesized and introduced into oligonucleotides. The oligonucleotide containing 2′-F,4′-C-OMe–araU exhibited improved nuclease resistance and RNA hybridizing selective ability relative to 2′-F–araU. In particular, when 2′-F,4′-C-OMe–araU inserted into C–H⋯F–C bonding-favorable 5′–uridine–purine–3′ steps, the modified oligonucleotide showed remarkable binding affinity and selectivity to RNA complements. Thus, 2′-F,4′-C-OMe–araU has valuable antisense properties and can be used as novel chemical modification for antisense therapeutic strategy.
Characterization of Cultivar Differences of Blueberry Wines Using GC-QTOF-MS and Metabolic Profiling Methods
Authors: Yuan Fang , Cheng Ke , Gao Jihui , Pan Siyi
Published: September 2018

A non-targeted volatile metabolomic approach based on the gas chromatography-quadrupole time of fight-mass spectrometry (GC-QTOF-MS) coupled with two different sample extraction techniques (solid phase extraction and solid phase microextraction) was developed. Combined mass spectra of blueberry wine samples, which originated from two different cultivars, were subjected to orthogonal partial least squares-discriminant analysis (OPLS-DA). Principal component analysis (PCA) reveals an excellent separation and OPLS-DA highlight metabolic features responsible for the separation. Metabolic features responsible for the observed separation were tentatively assigned to phenylethyl alcohol, cinnamyl alcohol, benzenepropanol, 3-hydroxy-benzenethanol, methyl eugenol, methyl isoeugenol, (E)-asarone, (Z)-asarone, and terpenes. Several of the selected markers enabled a distinction in secondary metabolism to be drawn between two blueberry cultivars. It highlights the metabolomic approaches to find out the influence of blueberry cultivar on a volatile composition in a complex blueberry wine matrix. The distinction in secondary metabolism indicated a possible O-methyltransferases activity difference among the two cultivars.
Senior Secondary School Food Literacy Education: Importance, Challenges, and Ways of Improving
Authors: Nanayakkara Janandani , Margerison Claire , Worsley Anthony
Published: September 2018

Food literacy education at senior secondary school can provide both immediate and long-term benefits for adolescents. The exploration of multiple stakeholder groups’ opinions regarding the importance, roles, and challenges of school food literacy education, and their suggestions for its improvement, will help the design and execution of future food literacy-related curricula and programmes. This study explored a broad range of Australian and international food, health, and education professionals’ opinions regarding senior secondary school food literacy education through an online survey. One hundred and fifty-five food, health, and education professionals completed this survey between April and October 2017. Overall, the respondents strongly supported the need for food literacy education for senior secondary school students. Their suggestions for improving this form of education included: incorporation of relevant and up-to-date content, the presence of strong practical components, offering food literacy as compulsory subjects or the incorporation of food literacy concepts into compulsory core subjects. Moreover, they proposed the active contribution of both internal and external stakeholders in the planning and delivery of this education to upgrade its quality and relevance. Overall, the findings suggest that a wide range of food, health, and education professionals are highly supportive of senior secondary school food literacy education and their constructive suggestions should be considered in school food literacy education enhancement efforts. Education authorities should seek ways of involving different stakeholders, including food-related professionals, in the design and delivery of food literacy education, and future studies should explore the best mechanisms for such involvement.
Plasma Treatment of Ovarian Cancer Cells Mitigates Their Immuno-Modulatory Products Active on THP-1 Monocytes
Authors: Bekeschus Sander , Wulf Can Pascal , Freund Eric , Koensgen Dominique , Mustea Alexander , Weltmann Klaus-Dieter , Stope Matthias B.
Published: September 2018

Cancers modulate their microenvironment to favor their growth. In particular, monocytes and macrophages are targeted by immuno-modulatory molecules installed by adjacent tumor cells such as ovarian carcinomas. Cold physical plasma has recently gained attention as innovative tumor therapy. We confirmed this for the OVCAR-3 and SKOV-3 ovarian cancer cell lines in a caspase 3/7 independent and dependent manner, respectively. To elaborate whether plasma exposure interferes with their immunomodulatory properties, supernatants of control and plasma-treated tumor cells were added to human THP-1 monocyte cultures. In the latter, modest effects on intracellular oxidation or short-term metabolic activity were observed. By contrast, supernatants of plasma-treated cancer cells abrogated significant changes in morphological and phenotypic features of THP-1 cells compared to those cultured with supernatants of non-treated tumor cell counterparts. This included cell motility and morphology, and modulated expression patterns of nine cell surface markers known to be involved in monocyte activation. This was particularly pronounced in SKOV-3 cells. Further analysis of tumor cell supernatants indicated roles of small particles and interleukin 8 and 18, with MCP1 presumably driving activation in monocytes. Altogether, our results suggest plasma treatment to alleviate immunomodulatory secretory products of ovarian cancer cells is important for driving a distinct myeloid cell phenotype.
Soft Islamophobia
Authors: Islam Namira
Published: September 2018

This article explores Islamophobia as a system of oppression by examining policies and practices found in liberal or left-leaning “anti-Islamophobia” spaces in the United States that limit the efficacy of the fight for justice. These include the reification of “Muslim” as a cultural category of brown foreigners, the lack of structural analysis around anti-Muslim hate, and the discomfort with and erasure of Islam as a faith in favor of “cultural” Muslims. The article then briefly proposes alternate ways forward that center the Muslims most affected by the intersections of race, class, gender, and religion in work against Islamophobia so that advocates can more effectively and directly address a system of Islamophobia.
Wind Direction Inversion from Narrow-Beam HF Radar Backscatter Signals in Low and High Wind Conditions at Different Radar Frequencies
Authors: Shen Wei , Gurgel Klaus-Werner
Published: September 2018

Land-based, high-frequency (HF) surface wave radar has the unique capability of monitoring coastal surface parameters, such as current, waves, and wind, up to 200 km off the coast. The Doppler spectrum of the backscattered radar signal is characterized by two strong peaks that are caused by the Bragg-resonant scattering from the ocean surface. The wavelength of Bragg resonant waves is exactly half the radio wavelength (grazing incidence), and these waves are located at the higher frequency part of the wave spectral distribution. When HF radar operates at higher frequencies, the resonant waves are relatively shorter waves, which are more sensitive to a change in wind direction, and they rapidly respond to local wind excitation and a change in wind direction. When the radar operates at lower frequencies, the corresponding resonant waves are relatively longer and take longer time to respond to a change in wind direction due to the progress of wave growth from short waves to long waves. For the wind inversion from HF radar backscatter signals, the accuracy of wind measurement is also relevant to radar frequency. In this paper, a pattern-fitting method for extracting wind direction by estimating the wave spreading parameter is presented, and a comparison of the pattern-fitting method and a conventional method is given as well, which concludes that the pattern-fitting method presents better results than the conventional method. In order to analyze the wind direction inversion from radar backscatter signals under different wind conditions and at different radar frequencies, two radar experiments accomplished in Norway and Italy are introduced, and the results of wind direction inversion are presented. In the two experiments, the radar worked at 27.68 MHz and 12 MHz, respectively, and the wind conditions at the sea surface were quite different. In the experiment in Norway, 67.4% of the wind records were higher than 5 m/s, while, in the experiment in Italy, only 18.9% of the wind records were higher than 5 m/s. All these factors affect the accuracy of wind direction inversion. The paper analyzes the radar data and draws a conclusion on the influencing factor of wind direction inversion.
Surface Moisture and Vegetation Cover Analysis for Drought Monitoring in the Southern Kruger National Park Using Sentinel-1, Sentinel-2, and Landsat-8
Authors: Urban Marcel , Berger Christian , Mudau Tami E. , Heckel Kai , Truckenbrodt John , Onyango Odipo Victor , Smit Izak P. J. , Schmullius Christiane
Published: September 2018

During the southern summer season of 2015 and 2016, South Africa experienced one of the most severe meteorological droughts since the start of climate recording, due to an exceptionally strong El Niño event. To investigate spatiotemporal dynamics of surface moisture and vegetation structure, data from ESA’s Copernicus Sentinel-1/-2 and NASA’s Landsat-8 for the period between March 2015 and November 2017 were utilized. In combination, these radar and optical satellite systems provide promising data with high spatial and temporal resolution. Sentinel-1 C-band data was exploited to derive surface moisture based on a hyper-temporal co-polarized (vertical-vertical—VV) radar backscatter change detection approach, describing dynamics between dry and wet seasons. Vegetation information from a TLS (Terrestrial Laser Scanner)-derived canopy height model (CHM), as well as the normalized difference vegetation index (NDVI) from Sentinel-2 and Landsat-8, were utilized to analyze vegetation structure types and dynamics with respect to the surface moisture index (SurfMI). Our results indicate that our combined radar–optical approach allows for a separation and retrieval of surface moisture conditions suitable for drought monitoring. Moreover, we conclude that it is crucial for the development of a drought monitoring system for savanna ecosystems to integrate land cover and vegetation information for analyzing surface moisture dynamics derived from Earth observation time series.
Exploiting Fine-Grained Subcarrier Information for Device-Free Localization in Wireless Sensor Networks
Authors: Guo Yan , Yu Dongping , Li Ning
Published: September 2018

Device-free localization (DFL) that aims to localize targets without carrying any electronic devices is addressed as an emerging and promising research topic. DFL techniques estimate the locations of transceiver-free targets by analyzing their shadowing effects on the radio signals that travel through the area of interest. Recently, compressive sensing (CS) theory has been applied in DFL to reduce the number of measurements by exploiting the inherent spatial sparsity of target locations. In this paper, we propose a novel CS-based multi-target DFL method to leverage the frequency diversity of fine-grained subcarrier information. Specifically, we build the dictionaries of multiple channels based on the saddle surface model and formulate the multi-target DFL as a joint sparse recovery problem. To estimate the location vector, an iterative location vector estimation algorithm is developed under the multitask Bayesian compressive sensing (MBCS) framework. Compared with the state-of-the-art CS-based multi-target DFL approaches, simulation results validate the superiority of the proposed algorithm.
An Image Signal Accumulation Multi-Collection-Gate Image Sensor Operating at 25 Mfps with 32 × 32 Pixels and 1220 In-Pixel Frame Memory
Authors: Dao Vu , Ngo Nguyen , Nguyen Anh , Morimoto Kazuhiro , Shimonomura Kazuhiro , Goetschalckx Paul , Haspeslagh Luc , De Moor Piet , Takehara Kohsei , Etoh Takeharu
Published: September 2018

The paper presents an ultra-high-speed image sensor for motion pictures of reproducible events emitting very weak light. The sensor is backside-illuminated. Each pixel is equipped with the multiple collection gates (MCG) at the center of the front side. Each collection gate is connected to an in-pixel large memory unit, which can accumulate image signals captured by repetitive imaging. The combination of the backside illumination, image signal accumulation, and slow readout from the in-pixel signal storage after an image capturing operation offers a very high sensitivity. Pipeline signal transfer from the MCG to the in-pixel memory units enables the sensor to achieve a large frame count and a very high frame rate at the same time. A test sensor was fabricated with a pixel count of 32 × 32 pixels. Each pixel is equipped with four collection gates, each connected to a memory unit with 305 elements; thus, with a total frame count of 1220 (305 × 4) frames. The test camera achieved 25 Mfps, while the sensor was designed to operate at 50 Mfps.
Wireless Channel Propagation Characteristics and Modeling Research in Rice Field Sensor Networks
Authors: Gao Zhenran , Li Weijing , Zhu Yan , Tian Yongchao , Pang Fangrong , Cao Weixing , Ni Jun
Published: September 2018

Wireless channel propagation characteristics and models are important to ensure the communication quality of wireless sensor networks in agriculture. Wireless channel attenuation experiments were carried out at different node antenna heights (0.8 m, 1.2 m, 1.6 m, and 2.0 m) in the tillering, jointing, and grain filling stages of rice fields. We studied the path loss variation trends at different transmission distances and analyzed the differences between estimated values and measured values of path loss in a free space model and a two-ray model. Regression analysis of measured path loss values was used to establish a one-slope log-distance model and propose a modified two-slope log-distance model. The attenuation speed in wireless channel propagation in rice fields intensified with rice developmental stage and the transmission range had monotone increases with changes in antenna height. The relative error (RE) of estimation in the free space model and the two-ray model under four heights ranged from 6.48–15.49% and 2.09–13.51%, respectively, and these two models were inadequate for estimating wireless channel path loss in rice fields. The ranges of estimated RE for the one-slope and modified two-slope log-distance models during the three rice developmental stages were 2.40–2.25% and 1.89–1.31%, respectively. The one-slope and modified two-slope log-distance model had better applicability for modeling of wireless channels in rice fields. The estimated RE values for the modified two-slope log-distance model were all less than 2%, which improved the performance of the one-slope log-distance model. This validates that the modified two-slope log-distance model had better applicability in a rice field environment than the other models. These data provide a basis for modeling of sensor network channels and construction of wireless sensor networks in rice fields. Our results will aid in the design of effective rice field WSNs and increase the transmission quality in rice field sensor networks.
Entropy Correlation and Its Impacts on Data Aggregation in a Wireless Sensor Network
Authors: Nguyen Thi Thanh Nga , Nguyen Kim Khanh , Ngo Hong Son , Ngo Lam Trung
Published: September 2018

A correlation characteristic has significant potential advantages for the development of efficient communication protocols in wireless sensor networks (WSNs). To exploit the correlation in WSNs, the correlation model is required. However, most of the present correlation models are linear and distance-dependent. This paper proposes a general distance-independent entropy correlation model based on the relation between joint entropy and the number of members in a group. This relation is estimated using entropy of individual members and entropy correlation coefficients of member pairs. The proposed model is then applied to evaluate two data aggregation schemes in WSNs including data compression and representative schemes. In the data compression scheme, some main routing strategies are compared and evaluated to find the most appropriate strategy. In the representative scheme, with the desired distortion requirement, a method to calculate the number of representative nodes and the selection of these nodes are proposed. The practical validations showed the effectiveness of the proposed correlation model and data reduction schemes.