bluuebunny/crossref_metadata_2025 huggingface dataset:
Systematic review of the complement components as potential biomarkers of pre-eclampsia: pitfalls and opportunities
Authors: Balduit Andrea , Agostinis Chiara , Mangogna Alessandro , Zito Gabriella , Stampalija Tamara , Ricci Giuseppe , Bulla Roberta
Published: June 2024

The complement system (C) is a crucial component of the innate immune system. An increasing body of research has progressively shed light on the pivotal role of C in immunological tolerance at the feto-maternal interface. Excessive C activation or impaired C regulation may determine the onset of pregnancy-related pathological conditions, including pre-eclampsia (PE). Thus, several studies have investigated the presence of C components or split products in blood matrixes (i.e., plasma, serum), urine, and amniotic fluid in PE. In the current study, we systematically reviewed the currently available scientific literature reporting measurements of C components as circulating biomarkers in PE, based on a literature search using Pubmed, Scopus, and Embase databases. A total of 41 out of 456 studies were selected after full-text analysis. Fourteen studies (34.1%) were identified as measuring the blood concentrations of the classical pathway, 5 (12.1%) for the lectin pathway, 28 (68.3%) for the alternative pathway, 17 (41.5%) for the terminal pathway components, and 16 (39%) for C regulators. Retrieved results consistently reported C4, C3, and factor H reduction, and increased circulating levels of C4d, Bb, factor D, C3a, C5a, and C5b-9 in PE compared to normal pregnancies, depicting an overall scenario of excessive C activation and aberrant C regulation. With evidence of C activation and dysregulation, C-targeted therapy is an intriguing perspective in PE management. Moreover, we also discussed emerging pitfalls in C analysis, mainly due to a lack of experimental uniformity and biased cohort selection among different studies and laboratories, aiming to raise a more comprehensive awareness for future standardization.

Systematic review registration

https://www.crd.york.ac.uk/prospero/, identifier CRD42024503070.
Human dimensions in shellcraft: tool ownership differentiates taxa utilised and products produced
Authors: Militz Thane A. , Simard Nittya S. M. , Kinch Jeff , Southgate Paul C.
Published: June 2024

Throughout the Pacific Islands, shellcraft has begun to feature prominently in development intervention which aims to generate positive livelihood outcomes for coastal communities. This activity often involves the post-harvest processing of natural assets, namely shells and skeletal remains of marine taxa, into jewellery by individuals or small-sized enterprises. To foster participation in shellcraft, development intervention commonly involves gifting or subsidising physical assets. Yet knowledge of the natural (i.e., taxa) and physical (i.e., tools) assets required for shellcraft remains scant. An understanding of human dimensions in shellcraft pertaining to patterns in asset use, such as how tool ownership differentiates taxa utilised and products produced, is important for determining if access to a specific tool is necessary, or an encumbrance, for achieving a desired outcome. In this study, we identified tools used and variation in tool ownership among artisans engaged in shellcraft to afford an accurate and realistic understanding of the tools required to participate in this activity. We then evaluate how ownership of a specific tool or type of tool differentiates both taxa utilisation and product production for shellcraft. Results indicate ownership of neither all nor any specific tool was required to participate in shellcraft, with artisans typically owning a unique combination of tools. Furthermore, results identified that some specific tools were critical for affecting the attractiveness of utilising certain taxa or producing certain products. Implications of the significant relationships between specific tools and certain taxa or products are discussed in the context of development intervention and commonly articulated theories of change where shellcraft is linked to subsistence fishing, aquaculture, or tourism.
Correlation between motor function and health-related quality of life in early to mid-stage patients with Parkinson disease: a cross-sectional observational study
Authors: Ge Ying , Zhao Wowa , Zhang Lu , Zhao Xiaoyi , Shu Xuan , Li Jiawei , Liu Ying
Published: June 2024

Aim

To investigate the correlation between motor function and health-related quality of life (HrQOL) in early to mid-stage patients with Parkinson disease (PwP).

Methods

This cross-sectional study recruited PwP from April 2020 to December 2023 at the outpatient clinic of Peking Union Medical College Hospital in Beijing, China. The motor symptoms were assessed using Movement Disorder Society–sponsored revision of the Unified Parkinson’s Disease Rating Scale (MDS-UPDRS) part 3. Balance function was evaluated using the Berg Balance Scale (BBS), and the risk of fall using Timed Up-and-Go test (TUG), and Five Times Sit-to-Stand test (FTSST). Freezing of gait questionnaire (FOGQ) was used to evaluate the severity of gait. The Intelligent Device for Energy Expenditure and Physical Activity (IDEEA) recorded gait cycle parameters, and the isokinetic dynamometer measured muscle strength. The Parkinson’s Disease Questionnaire-39 (PDQ-39) was used to measure HrQOL. All assessments were tested during the on state. Spearman correlation was conducted to evaluate the correlation between motor function and HrQOL.

Results

243 patients with mean age of 69.33 years were enrolled. The PDQ-39 score was strongly correlated with FOG in H&Y stage III (r = 0.653, p < 0.001) and moderately correlated in H&Y stage I (r = 0.471, p < 0.001) and H&Y stage II (r = 0.386, p < 0.001). Furthermore, the FOG was strongly correlated with mobility domain at H&Y stage III (r = 0.694, p < 0.001) and moderately correlated at H&Y stage I (r = 0.431, p < 0.001) and H&Y stage II (r = 0.434, p < 0.001). All motor function scores were correlated with PDQ-39 scores at H&Y stage III (p < 0.05).

Conclusion

Motor function correlated with HrQOL in early to mid-stage PwP, and FOG was the main factor, especially affecting mobility, activities of daily life and communication. HrQOL in patients at different disease stages were variously affected by motor function, and HrQOL and multiple dimensions was significantly associated with motor function in patients at H&Y stage III.
Case report: Symmetrical and increased lateral sway-based walking training for patients with corpus callosum infarction: a case series
Authors: Jiang Ying , Chen Sijing , Wu Dan , He Wei , Ma Xiaoqing , Zhang Lixia , Zhang Qian
Published: June 2024

Introduction

Corpus callosum injury is a rare type of injury that occurs after a stroke and can cause lower limb dysfunction and a decrease in activities of daily living ability. Furthermore, there are no studies that focus on the progress in rehabilitation of the lower limb dysfunction caused by infarction in the corpus callosum and the effective treatment plans for this condition. We aimed to present a report of two patients with lower limb dysfunction caused by corpus callosum infarction after a stroke and a walking training method.

Methods

We implemented a walking training method that prioritizes bilateral symmetry and increases lateral swaying before the patients established sitting/standing balance. The plan is a rapid and effective method for improving walking dysfunction caused by corpus callosum infarction.

Case characteristics

Following sudden corpus callosum infarction, both patients experienced a significant reduction in lower limb motor function scores and exhibited evident gait disorders. Scale evaluations confirmed that walking training based on symmetrical and increased lateral sway for patients with lower limb motor dysfunction after corpus callosum infarction led to significant symptom improvement.

Conclusion

We report two cases of sudden motor dysfunction in patients with corpus callosum infarction. Symmetrical and increased lateral sway-based walking training resulted in substantial symptom improvement, as confirmed by scale assessments.
Controlled ultrasonic interventions through the human skull
Authors: Wilson Matthew G. , Riis Thomas S. , Kubanek Jan
Published: June 2024

Transcranial focused ultrasound enables precise and non-invasive manipulations of deep brain circuits in humans, promising to provide safe and effective treatments of various neurological and mental health conditions. Ultrasound focused to deep brain targets can be used to modulate neural activity directly or localize the release of psychoactive drugs. However, these applications have been impeded by a key barrier—the human skull, which attenuates ultrasound strongly and unpredictably. To address this issue, we have developed an ultrasound-based approach that directly measures and compensates for the ultrasound attenuation by the skull. No additional skull imaging, simulations, assumptions, or free parameters are necessary; the method measures the attenuation directly by emitting a pulse of ultrasound from an array on one side of the head and measuring with an array on the opposite side. Here, we apply this emerging method to two primary future uses—neuromodulation and local drug release. Specifically, we show that the correction enables effective stimulation of peripheral nerves and effective release of propofol from nanoparticle carriers through an ex vivo human skull. Neither application was effective without the correction. Moreover, the effects show the expected dose-response relationship and targeting specificity. This article highlights the need for precise control of ultrasound intensity within the skull and provides a direct and practical approach for addressing this lingering barrier.
Washed microbiota transplantation improves sleep quality in patients with sleep disorder by the gut-brain axis
Authors: He Hongxin , Li Manqing , Qiu Yifan , Wu Zhiqing , Wu Lei
Published: June 2024

Background

The clinical impact of washed microbiota transplantation (WMT) from healthy donors in sleep disorder (SD) patients is unclear. This study aimed to investigate the effect of WMT in SD patients.

Methods

The clinical data were collected from patients with different indications receiving 1–3 courses of WMT, divided into two groups by 7 points of PSQI scale. The score of PQSI and SF-36 scale was used to assess the improvement in sleep quality and life quality among patients with sleep disorders following WMT. Finally, 16S rRNA gene amplicon sequencing was performed on fecal samples of patients with sleep disorders before and after WMT.

Results

WMT significantly improved sleep quality in patients with sleep disorder in the short and medium term. WMT significantly improved sleep latency, sleep time and total score in the short term. WMT significantly improved sleep quality and total score in the medium term. In terms of sleep quality and sleep latency, the improvement value also increased with the increase of treatment course, and the improvement effect of multiple treatment course was better than that of single and double treatment course. In the total score, the improvement effect of double and multiple treatment was better than that of single treatment. WMT also improved quality of life in the sleep disorder group. WMT significantly improved general health, vitality, social function and mental health in the short term. WMT significantly improved role-physical, general health, vitality, and mental health in the medium term. WMT regulated the disturbed gut microbiota in patients with sleep disorders. In the normal sleep group, WMT had no effect on the decline of sleep quality in the short, medium and long term, and had an improving effect on the quality of life.

Conclusion

WMT could significantly improve sleep quality and life quality in patients with sleep disorders with no adverse events. The improvement in sleep quality resulting from WMT could lead to an overall enhancement in life quality. WMT could be a potentially effective treatment for patients with sleep disorders by regulating the gut microbiota.
Cholangiocarcinoma, sequential chemotherapy, and prognostic tests
Authors: Bruckner Howard W. , De Jager Robert , Knopf Elisheva , Bassali Fred , Book Abe , Gurell Daniel , Nghiem Van , Schwartz Myron , Hirschfeld Azriel
Published: June 2024

Introduction

Routine blood tests are prognostic tests for patients with cholangiocarcinoma. New drug regimens may produce a median overall survival of 2 years or more.

Methods

This single practice, IRB-approved, phase II trial examines prognostic tests, Kaplan-Meier survival, and univariate Cox regression analyses. Eligibility requires: intent-to-treat; signed consent; advanced measurable intrahepatic cholangiocarcinoma, with or without resistance to the test drugs; any adult age; performance status 0–2; and expected survival of ≥ 6 weeks. Biweekly treatment, with 1/3 of standard dosages in mg/M2, includes: Gemcitabine 500; 5-Fluorouracil 1200 over 24 hours; Leucovorin 180; Irinotecan 80; and on day 2, Oxaliplatin 40. On progression, drugs are added on day 2: first, Docetaxel 25 precedes Oxaliplatin, with or without Mitomycin C 6 after Oxaliplatin. The next sequential additions are day 1, Cetuximab 400 total mg, then 200 mg weekly, and then Bevacizumab 10 mg/kg is substituted for Cetuximab (FDA IND# 119005).

Results

For 35 patients, 19 with 1–2 lines of prior therapy, resistant tumors, and 16 no prior therapy, survival at 24-months is ≥ 72 and ≥ 58%, respectively. For 14 patients aged ≥ 70 years, ≥ 63% survive 24 months, P = 0.28. Validated tests that predict ≤ 6-month survivals find median survival times of 17-months through > 2-years when compared to patients with favorable tests: Neutrophils lymphocyte ratio > 3.0, HR = 6.54, P < 6.4x10–3; absolute neutrophil count > 8000/μl, HR = 4.95, P < 6.5x10–3; serum albumin < 3.5 g/dl, HR = 4.10, P < 0.03; and lymphocyte monocyte ratio< 2.1, HR = 1.6, P = 0.50. Overall, the 76 (60–90)% of patients with 0–2 out of 4 high risk tests survive ≥ 24 months, (P = 7.1x10–3). Treatments produce neither hospitalization, neutropenic fever, severe enteritis, nor severe neuropathies.

Conclusion

Two-year survival is replicable and predictable. Findings warrant phase III validation tests of sequential regimens, re-challenge with recombination, low dosages, and blood tests that are associated with lethal mechanisms that impair response and survival.
Long-term oncologic outcomes following breast cancer surgery in adolescents and young adults: a single-center retrospective analysis
Authors: Liu Xin , Ma Zengyan , Chu Hongwu , Nie Weihong , Sun Guoxin , Zhao Kaihua , Zou Xiao
Published: June 2024

Background

Breast cancer (BC) in adolescents and young adults (AYAs, aged 15–39 years), remains inadequately understood. The incidence of BC in AYAs has been steadily increasing, making it the second leading cause of cancer-related mortality among females aged 0–39 globally. This study aimed to elucidate the clinical characteristics and long-term outcomes of AYAs and older adults (OAs, aged > 39 years) with BC who underwent surgery.

Methods

From January 2011 to June 2017, BC patients who underwent surgery were enrolled in this study and divided into AYA group and OA group. Clinical characteristics, recurrence-free survival (RFS), and overall survival (OS) were compared between these two groups, both before and after propensity score matching (PSM). Univariate and multivariate Cox proportional hazard regression analyses were performed to assess the influence of age on OS and RFS.

Results

Compared to the OA group, the AYA group exhibited a younger age at menarche (p < 0.001), a lower prevalence of menopausal status (p < 0.001), a reduced occurrence of comorbid conditions (p < 0.001), fewer instances of undergoing mastectomy (p = 0.031), a higher incidence of Triple-Negative Breast Cancer (TNBC) (p = 0.046), and elevated Ki-67 levels (p = 0.036). In terms of prognostic outcomes, within the study cohort, AYAs had a higher mortality rate and poorer long-term survival compared to OAs, both before and after PSM. In the PSM cohort, AYAs experienced a significantly shorter mean OS (p < 0.001) and RFS (p < 0.001). Young age (15–39 years) emerged as an independent risk factor for OS (HR 2.659, 95% CI 1.385–5.106, p = 0.003) and RFS (HR 3.235, 95% CI 2.085–5.022, p < 0.001) in BC patients following surgery.

Conclusion

Significant differences were identified in the clinicopathological characteristics between AYA and OA patients with BC. In comparison to OA patients, AYA patients exhibited a less favorable long-term prognosis, with young age emerging as an independent prognostic risk factor for both OS and RFS in BC patients following surgery. Further investigations are warranted to develop age-specific therapeutic approaches for AYA BC patients.
Case report: Spinal cord stimulation for pain relief in two patients with locally recurrent pelvic malignancy
Authors: Salem Yousif , West Charles T. , West Malcolm , Yano Hideaki , Fernandes Paul , Vajramani Girish , Mirnezami Alexander
Published: June 2024

Introduction

Chronic cancer-related pain from locally recurrent infiltrative cancers within the bony confines of the pelvis is a devastating and hard to manage condition that can be refractory to many conventional pain management methods. Spinal cord stimulation (SCS) is an evolving and safe method of pain management and can be trialled in a quick and well-tolerated operation under local anaesthesia. To date, this has not been reported in the setting of locally recurrent inoperable pelvic cancers.

Case description

In the present study, we report two cases of patients with severe back and lower limb pain resulting from recurrent anal and rectal cancers involving the right lumbar and sacral nerve roots as well as the bony sacrum, which severely affected quality of life and daily functioning.

Discussion

Following successful SCS, effective pain relief was observed.

Conclusion

SCS could represent an effective supplementary or alternative technique to conventional pain management in this challenging group of patients, especially if other available methods have been exhausted.
Surgical outcomes of proximal femoral bone cysts in pediatric patients: a retrospective study of 41 cases
Authors: Li Taichun , Dai Zhenzhen , Ma Qichao , Zhou Han , Li Hai , Zhang Ziming
Published: June 2024

Purpose

The aim of this study was to evaluate the surgical outcomes of proximal femoral bone cysts in pediatric patients.

Methods

We retrospectively analyzed 41 pediatric patients (31 males and 10 females, mean age 7.47 ± 2.67 years, range 2.03–14.67 years) diagnosed with proximal femoral bone cysts treated at a single institute between March 2009 and November 2021. Data included demographics, preoperative details, intraoperative conditions, surgical techniques, postoperative outcomes, recurrence, and complications.

Results

Of the participants, 68% presented with simple bone cysts and 32% with aneurysmal bone cysts. Prior to surgery, 32% exhibited pathological fractures. Surgical methods included lesion curettage, defect filling using allograft bone and Minimally-Invasive Injectable Graft ×3, and varied fixation techniques. Postoperative recurrence (17%) was associated with cyst location between the capital femoral epiphysis and the linea intertrochanterica (P = 0.010). At the final assessment (mean follow-up: 26.51 ± 18.99 months), all showed radiological bony union with 93% rated as “good” and 7% as ‘fair’ based on Ratliff hip scores. Complications arose in 20% of patients, significantly correlated with prior pathological fractures (P = 0.007) and their association with the linea intertrochanterica (P = 0.004). Those with fractures reported higher intraoperative blood loss (P = 0.015) and longer surgery durations (P = 0.012) compared to those without.

Conclusion

Treating pediatric proximal femoral bone cysts using techniques such as lesion curettage, defect filling, and selective internal fixation yields favorable outcomes. The presence of pathological fractures can prolong surgical time, increase intraoperative blood loss, and elevate postoperative complication risks. Hence, early surgical intervention for these cysts is recommended to prevent fractures.
Exploring the therapeutic potential of Nelumbo nucifera leaf extract against amyloid-beta-induced toxicity in the Caenorhabditis elegans model of Alzheimer’s disease
Authors: Wen Yong-Ping , Fu Hai-Jun , Chen Qin , Lan Cai , Qin Da-Lian , Wu Jian-Ming , Wu An-Guo , Zhou Xiao-Gang
Published: June 2024

Introduction: Alzheimer’s disease (AD) represents a critical global health challenge with limited therapeutic options, prompting the exploration of alternative strategies. A key pathology in AD involves amyloid beta (Aβ) aggregation, and targeting both Aβ aggregation and oxidative stress is crucial for effective intervention. Natural compounds from medicinal and food sources have emerged as potential preventive and therapeutic agents, with Nelumbo nucifera leaf extract (NLE) showing promising properties.

Methods: In this study, we utilized transgenic Caenorhabditis elegans (C. elegans) models to investigate the potential of NLE in countering AD and to elucidate the underlying mechanisms. Various assays were employed to assess paralysis rates, food-searching capabilities, Aβ aggregate accumulation, oxidative stress, lifespan under stress conditions, and the expression of stress-resistance-related proteins. Additionally, autophagy induction was evaluated by measuring P62 levels and the formation of LGG-1+ structures, with RNAi-mediated inhibition of autophagy-related genes to confirm the mechanisms involved.

Results: The results demonstrated that NLE significantly reduced paralysis rates in CL4176 and CL2006 worms while enhancing food-searching capabilities in CL2355 worms. NLE also attenuated Aβ aggregate accumulation and mitigated Aβ-induced oxidative stress in C. elegans. Furthermore, NLE extended the lifespan of worms under oxidative and thermal stress conditions, while concurrently increasing the expression of stress-resistance-related proteins, including SOD-3, GST-4, HSP-4, and HSP-6. Moreover, NLE induced autophagy in C. elegans, as evidenced by reduced P62 levels in BC12921 worms and the formation of LGG-1+ structures in DA2123 worms. The RNAi-mediated inhibition of autophagy-related genes, such as bec-1 and vps-34, negated the protective effects of NLE against Aβ-induced paralysis and aggregate accumulation.

Discussion: These findings suggest that NLE ameliorates Aβ-induced toxicity by activating autophagy in C. elegans. The study underscores the potential of NLE as a promising candidate for further investigation in AD management, offering multifaceted approaches to mitigate AD-related pathology and stress-related challenges.
The reliability and discriminant validity of physical, technical, and perceptual-physiological measures during a game-specific basketball activity simulation protocol
Authors: Ferioli Davide , Alcaraz Pedro E. , Freitas Tomás T. , Trimarchi Fabio , Conte Daniele , Formica Lorenzo , Chung Linda H. , Scanlan Aaron T.
Published: June 2024

Activity simulation protocols offer useful applications in research and practice; however, the specificity of such protocols to basketball game-play is currently lacking. Consequently, this study aimed to develop a game-specific basketball activity simulation protocol representative of typical playing durations and assess its reliability and discriminant validity. The simulation protocol was modified from an original version (i.e., Basketball Exercise Simulation Test) to incorporate regular breaks indicative of time-outs, free-throws, and substitutions. Twelve competitive male and female adult basketball players competing in the fourth or fifth Spanish basketball division underwent repeated trials of the simulation protocol (min. 4 to max. 14 days apart) for reliability analyses. In turn, 13 competitive male (fifth division), 9 competitive female (fourth division), and 13 recreational male adult basketball players completed the simulation protocol to assess discriminant validity via comparisons between sexes (competitive players) and playing levels (males). A range of physical, technical, and perceptual-physiological variables were collected during and following the simulation protocol. Several physical and heart rate variables displayed the strongest reliability (intraclass correlation coefficient [ICC] = 0.72–0.96; coefficient of variation [CV] = 1.78–6.75%), with physical decrement, technical, blood lactate concentration, and rating of perceived exertion (RPE) variables having the weakest (ICC = 0.52–0.75; CV = 10.34–30.85%). Regarding discriminant analyses between sexes, males demonstrated significantly greater physical outputs in several variables and lower RPE compared to females (p < 0.05, moderate-to-large effects). Comparisons between playing levels revealed competitive males had significantly greater physical outputs across many variables, alongside higher mean heart rate and lower RPE than recreational males (p < 0.05, moderate-to-large effects). This study presents a novel game-specific basketball activity simulation protocol replicating actual playing durations and game configurations that might be successfully applied for both training and research purposes. Reliability statistics are provided for several variables to inform end-users on potential measurement error when implementing the simulation protocol. Discriminant validity of the simulation protocol was supported for several variables, suggesting it may hold practical utility in benchmarking or selecting players. Future research on this topic is encouraged examining wider samples of male and female basketball players at different levels as well as additional forms of validity for the protocol.
Circulating inflammatory cytokines influencing schizophrenia: a Mendelian randomization study
Authors: Li Yao-Ting , Zeng Xuezhen
Published: June 2024

Introduction

Schizophrenia (SCZ) is a severe psychiatric disorder whose pathophysiology remains elusive. Recent investigations have underscored the significance of systemic inflammation, particularly the impact of circulating inflammatory proteins, in SCZ.

Methods

This study explores the potential causal association between certain inflammatory proteins and SCZ. Bidirectional Mendelian randomization (MR) analyses were conducted utilizing data from expansive genome-wide association studies (GWAS). Data regarding circulating inflammatory proteins were sourced from the GWAS Catalog database, encompassing 91 inflammatory cytokines. SCZ-related data were derived from the Finngen database, incorporating 47,696 cases and 359,290 controls. Analytical methods such as inverse variance weighted, MR-Egger, weighted median, simple mode, and weighted mode were employed to evaluate the association between inflammatory cytokines and SCZ. Sensitivity analyses were also performed to affirm the robustness of the results.

Results

Following FDR adjustment, significant associations were observed between levels of inflammatory cytokines, including Fibroblast Growth Factor 5 (OR = 1.140, 95%CI = 1.045, 1.243, p = 0.003, FDR=0.015), C-C Motif Chemokine 4 (OR = 0.888, 95%CI = 0.816, 0.967, p = 0.006, FDR = 0.015), C-X-C Motif Chemokine 1 (OR = 0.833, 95%CI = 0.721, 0.962, p = 0.013, FDR = 0.064), and C-X-C Motif Chemokine 5 (OR = 0.870, 95%CI = 0.778, 0.973, p = 0.015, FDR = 0.074), and the risk of SCZ.

Conclusion

Our results from MR analysis suggest a potential causal link between circulating inflammatory cytokines and SCZ, thereby enriching our understanding of the interactions between inflammation and SCZ. Furthermore, these insights provide a valuable foundation for devising therapeutic strategies targeting inflammation.
Spatiotemporal parameters and kinematics differ between race stages in trail running—a field study
Authors: Genitrini Matteo , Fritz Julian , Stöggl Thomas , Schwameder Hermann
Published: June 2024

Introduction

Trail running is an emerging discipline with relatively few studies performed in ecological conditions. The aim of this work was to investigate if and how spatiotemporal parameters (STP) and kinematics differ between initial and final stage of a field trial.

Methods

Twenty trail runners (10 F, 10 M) were recruited and ran a solo 9.1 km trial. During the test, participants wore a GPS watch and an IMU-based motion capture system. Running speed, elapsed time, STP and kinematics were compared between initial and final stage, separately for uphill (UH) and downhill (DH) sections.

Results

Running speed decreased in the final stage (p<0.05). Total test time was more correlated to the time elapsed in UH sections. In the final stage and in both UH and DH sections, contact time and duty factor increased, whilst stride length and flight time decreased (p<0.05). In the final stage, ankle joint was more dorsiflexed in stance and swing phases in UH sections and stance phase only in DH sections (p<0.05). In the final stage, knee joint was less extended in swing phase in UH and DH sections, as well as less extended in stance in UH sections (p<0.05). In the final stage, hip joint was less flexed in the swing phase in UH and DH sections (p<0.05). In the final stage, forward trunk lean was higher across the entire gait cycle in in UH sections (p<0.05). Trunk contralateral axial rotation was lower, in DH sections (p<0.05).

Discussion

During the final stage, results indicate a less efficient propulsion phase, in both UH and DH sections. In UH sections, results suggest lower energy generation at the ankle joint. In DH sections, results suggest that the kinematics of swing leg may play a role in sub-optimizing propulsion phase. This study demonstrates how, in UH and DH sections, similar changes in spatiotemporal parameters can be elicited by dissimilar changes in running kinematics. To optimize performance in trail running, coaches and practitioners are advised to work on different (incline-specific) aspects of running technique.
Resection of the tumor in the trigone of the lateral ventricle via the contralateral posterior interhemispheric transfalcine transprecuneus approach with multi-modern neurosurgery technology: a case report
Authors: Song Yunfei , Wang Zhen , Zhang Jun , Cui Xiaopeng , Wu Zhuolin , Zhao Zilin , Chen Yan , Zhang Suqin , Zhu Xiaowei , Wang Zhitao , Zhang Huijie , Gao Chao , Yang Shuyuan , Zhao Yan , Yang Xinyu
Published: June 2024

Choroid plexus papilloma (CPP) is a rare benign intracranial tumor origin that predominantly manifests in the lateral ventricle in children, accounting for 0.3%–0.6% of all primary intracranial tumors. It is extremely rare to have the CPP in the trigone of the lateral ventricle through the contralateral posterior interhemispheric transfalcine transprecuneus approach (PITTA). Herein, we report this rare case. A 7-year-old girl presented with headache. Magnetic resonance imaging of the brain showed periatrial lesions, and histopathological examination confirmed CPP (WHO grade I). The contralateral PITTA is a safe, effective, reasonable, and appropriate for some lesions in the trigone of the lateral ventricle. It provides a wider surgical angle (especially for the lateral extension) and reduces the risk of disturbance of the optic radiation compared with the conventional approaches. The use of multiple modern neurosurgical techniques, including interventional embolization, intraoperative navigation, microscope, and electrophysiological monitoring, make the procedure much easier and more accurate, and the neuroendoscope adds to the visualization of the microscope and can reduce surgical complications.
Abdominal wound length influences the postoperative serum level of interleukin-6 and recovery of flatus passage among patients with colorectal cancer
Authors: Tsai Po-Li , Chen Jian-Syun , Lin Chi-Hsin , Hsu Tzu-Chi , Lin Yu-Wen , Chen Ming-Jen
Published: June 2024

Introduction

A mini-laparotomy for colorectal cancer (CRC) has been reported to shorten postoperative ileus (POI) and hospital stay. Interleukin-6 (IL-6) plays a role in intestinal tissue inflammation, leading to POI. This study investigated the effects of abdominal wounds and IL-6 levels on POI in patients having CRC surgery.

Materials and methods

Forty-three patients with CRC underwent bowel resection. Serum samples were collected preoperatively and at 2, 24, and 48 h after surgery for cytokine quantification by ELISA. Clinical data, including time from surgery to first passage of flatus and postoperative hospital stay, demographic and pathological data, and routine blood tests, were compared statistically with abdominal wound length and the postoperative increments of cytokines (designated as Δ).

Results

The length of the abdominal wound showed a significant correlation with clinical variables (length of operation time, time of first flatus passage, and length of postoperative hospital stay) and cytokine variables (IL-6(Δ2 h), IL-8(Δ2 h) and IL-10(Δ2 h). Linear regression analysis showed that the abdominal wound length significantly influenced the operation time, time of first flatus passage, and length of postoperative hospital stay (p < 0.001). The length of the abdominal wound showed a significant influence on the IL-6(Δ2 h) and IL-8(Δ2 h) (p < 0.001, respectively) but no influence on IL-10(Δ2 h). IL-6(Δ2 h), but not IL-8(Δ2 h), significantly influenced the time to first flatus passage and length of hospital stay (p = 0.007, p = 0.006, respectively). The mini-laparotomy approach (wound length <7 cm) led to significantly shortened operation time, time of first flatus passage, length of postoperative stay (p = 0.004, p = 0.003, p = 0.006, respectively) as well as reduced postoperative increment of IL-6(Δ2 h) (p = 0.015). The mini-laparotomy for anterior resection surgery significantly influenced operation time, time of first passage of flatus, length of postoperative stay, and IL-6(Δ2 h).

Conclusion

Our study is the first to report the complex interaction among the length of the abdominal wound, IL-6 serum level, recovery of the first passage of flatus, and postoperative hospital stay. These results suggest that smaller abdominal wounds and smaller postoperative IL-6 increments were associated with faster recovery of flatus passage and shorter hospital stays.
Intolerance of Uncertainty, Job Satisfaction and Work Performance in Turkish Healthcare Professionals: Mediating Role of Psychological Capital
Authors: Yıldırım Murat , Çağış Zafer Güney , Gómez-Salgado Juan
Published: June 2024

Objective: Psychological capital refers to internal resources including self-efficacy, hope, optimism and resilience to overcome adverse life events. The current study sought to examine the mediating role of psychological capital in the relationship between intolerance of uncertainty and job satisfaction and work performance in healthcare professionals.

Methods: Participants were 302 healthcare professionals [48% females; M(SD)age = 34.0 (7.5)] and completed measures of intolerance of uncertainty, psychological capital, work performance, and job satisfaction.

Results: The findings indicated that intolerance of uncertainty was negatively correlated with psychological capital, work performance, and job satisfaction, whereas psychological capital was positively correlated with job satisfaction and work performance. More importantly, the findings revealed that these relationships were mediated by psychological capital.

Conclusion: The results provide several contributions that help to understand the role of psychological capital in the relationship between intolerance to uncertainty and job satisfaction and work performance.
Natural Killer Cell Presence in Antibody-Mediated Rejection
Authors: Diebold Matthias , Farkash Evan A. , Barnes Jenna , Regele Heinz , Kozakowski Nicolas , Schatzl Martina , Mayer Katharina A. , Haindl Susanne , Vietzen Hannes , Hidalgo Luis G. , Halloran Philip F. , Eskandary Farsad , Böhmig Georg A.
Published: June 2024

Transcript analyses highlight an important contribution of natural killer (NK) cells to microvascular inflammation (MVI) in antibody-mediated rejection (ABMR), but only few immunohistologic studies have quantified their spatial distribution within graft tissue. This study included 86 kidney transplant recipients who underwent allograft biopsies for a positive donor-specific antibody (DSA) result. NK cells were visualized and quantified within glomeruli and peritubular capillaries (PTC), using immunohistochemistry for CD34 alongside CD16/T-bet double-staining. Staining results were analyzed in relation to histomorphology, microarray analysis utilizing the Molecular Microscope Diagnostic System, functional NK cell genetics, and clinical outcomes. The number of NK cells in glomeruli per mm2 glomerular area (NKglom) and PTC per mm2 cortical area (NKPTC) was substantially higher in biopsies with ABMR compared to those without rejection, and correlated with MVI scores (NKglom Spearman’s correlation coefficient [SCC] = 0.55, p < 0.001, NKPTC 0.69, p < 0.001). In parallel, NK cell counts correlated with molecular classifiers reflecting ABMR activity (ABMRprob: NKglom 0.59, NKPTC 0.75) and showed a trend towards higher levels in association with high functional FCGR3A and KLRC2 gene variants. Only NKPTC showed a marginally significant association with allograft function and survival. Our immunohistochemical results support the abundance of NK cells in DSA-positive ABMR.
Effects of Different Materials and Structures on Mechanical Properties of Hail Used in Aviation Testing
Authors: Liu Yewei , Zhang Lifen , Ge Xin , Liu Zhenxia
Published: June 2024

Hail absorption test of aeroengine is one of the important components of airworthiness certification. The accurate test data are closely related to the density and mechanical properties of the artificial hail used in airworthiness tests. Through experimental research, this study explores the impact of distilled water, carbonated water and deionized water on the density and mechanical properties of artificial hail. The study addresses the significant differences between the density and mechanical properties of artificial hail and natural hail in existing studies. Based on this, a new method for preparing airworthiness test hail is proposed. The results indicate that artificial hail samples with distilled water as the hail core and carbonated water as the hail shell have densities ranging from 0.87 cm3 to 0.89 cm3. Furthermore, the estimated average maximum compressive strength of samples is 6.538 MPa, with some samples as low as 3.681 MPa. The mechanical properties of this artificial hail are more similar to those of natural hail. This method can more realistically simulate natural hail environments and can be used for the fine design of airworthiness certification criteria.
Development of a Device and Algorithm Research for Akhal-Teke Activity Level Analysis
Authors: Chen Xuan , Li Fuzhong , Li Jinxing , Fan Qijie , Kwan Paul , Zheng Wenxin , Guo Leifeng
Published: June 2024

This study demonstrated that wearable devices can distinguish between different levels of horse activity, categorized into three types based on the horse’s gaits: low activity (standing), medium activity (walking), and high activity (trotting, cantering, and galloping). Current research in activity level classification predominantly relies on deep learning techniques, known for their effectiveness but also their demand for substantial data and computational resources. This study introduces a combined acceleration threshold behavior recognition method tailored for wearable hardware devices, enabling these devices to classify the activity levels of horses directly. The approach comprises three sequential phases: first, a combined acceleration interval counting method utilizing a non-linear segmentation strategy for preliminary classification; second, a statistical analysis of the variance among these segments, coupled with multi-level threshold processing; third, a method using variance-based proximity classification for recognition. The experimental results show that the initial stage achieved an accuracy of 87.55% using interval counting, the second stage reached 90.87% with variance analysis, and the third stage achieved 91.27% through variance-based proximity classification. When all three stages are combined, the classification accuracy improves to 92.74%. Extensive testing with the Xinjiang Wild Horse Group validated the feasibility of the proposed solution and demonstrated its practical applicability in real-world scenarios.
Towards Unlocking the Hidden Potentials of the Data-Centric AI Paradigm in the Modern Era
Authors: Majeed Abdul , Hwang Seong Oun
Published: June 2024

Data-centric artificial intelligence (DC-AI) is a modern paradigm that gives more priority to data quality enhancement, rather than only optimizing the complex codes of AI models. The DC-AI paradigm is expected to substantially advance the status of AI research and developments, which has been solely based on model-centric AI (MC-AI) over the past 30 years. Until present, there exists very little knowledge about DC-AI, and its significance in terms of solving real-world problems remains unexplored in the recent literature. In this technical note, we present the core aspects of DC-AI and MC-AI and discuss their interplay when used to solve some real-world problems. We discuss the potential scenarios/situations that require the integration of DC-AI with MC-AI to solve challenging problems in AI. We performed a case study on a real-world dataset to corroborate the potential of DC-AI in realistic scenarios and to prove its significance over MC-AI when either data are limited or their quality is poor. Afterward, we comprehensively discuss the challenges that currently hinder the realization of DC-AI, and we list promising avenues for future research and development concerning DC-AI. Lastly, we discuss the next-generation computing for DC-AI that can foster DC-AI-related developments and can help transition DC-AI from theory to practice. Our detailed analysis can guide AI practitioners toward exploring the undisclosed potential of DC-AI in the current AI-driven era.
Medical Scope of Biofilm and Quorum Sensing during Biofilm Formation: Systematic Review
Authors: Erkihun Mulat , Asmare Zelalem , Endalamew Kirubel , Getie Birhanu , Kiros Teklehaimanot , Berhan Ayenew
Published: June 2024

Biofilms are accumulations of microorganisms in an extracellular polymeric substance matrix which are composed of polysaccharides, proteins, lipids, and nucleic acids. Many bacteria can switch between a planktonic form and a biofilm form. The planktonic bacteria have relatively high cell growth and reproduction rates and have a reduced likelihood of survival but can adapt to occupy new habitats. The biofilm state appears to be a natural and predominant state of bacteria. The need for the formation of bacterial biofilm is that it enhances the tolerance of bacteria to harsh environmental conditions, thereby allowing bacteria to avoid being washed away by water flow or the bloodstream by simply attaching to a surface or tissue, and the EPS matrix protects bacteria cells, in deeper layers, against antimicrobial agents, probably by limiting the diffusion of these agents. Biofilm formation steps are initial contact/attachment to the surface, followed by micro-colony formation, maturation and formation of the architecture of the biofilm, and finally detachment/dispersion of the biofilm. Once formed, biofilm restricts bacterial mobility and increases cell density. Secretions of autoinducers into the environment are critical for cross-signaling between bacteria. This cross-talk is called quorum sensing (QS). Quorum sensing is a cell–cell communication mechanism between bacteria that allows specific processes to be controlled, such as biofilm formation and virulence factor expression. Bacterial quorum sensing signaling mainly consists of acyl-homoserine lactones (produced by Gram-negatives), autoinducing peptides (produced by Gram-positives), and autoinducer-2 (produced by both Gram-negatives and Gram-positives). Therefore, this review is aimed at how bacterial biofilms work and are formed.
Extraction Strategies from Black Alloy Leachate: A Comparative Study of Solvent Extractants
Authors: Koo Namho , Kim Byungseon , Kim Hong-In , Kwon Kyungjung
Published: June 2024

Recycling spent lithium-ion batteries (LIBs) is crucial to prevent environmental pollution and recover valuable metals. Traditional methods for recycling spent LIBs include hydrometallurgy and pyrometallurgy. Among these methods, solvent extraction can selectively extract valuable metals in spent LIB leachate. Meanwhile, spent LIBs that underwent pyrometallurgical treatment generate a so-called ‘black alloy’ of Ni, Co, Cu, and so on. These elements in the black alloy need to be separated by solvent extraction and there have been few studies on extracting valuable metals from black alloy. Therefore, it is necessary to examine the extraction behavior of elements in black alloy and optimize the solvent extraction process to recover valuable metals. In this paper, four types of organic extractants are used to extract metals from simulated black alloy leachate: di-(2ethylhexyl) phosphoric acid (D2EHPA), bis-(2,4,4-trimethylpentyl) phosphinic acid (Cyanex272), 2-ethylhexyl phosphonic acid mono-2-ethylhexyl ester (PC88A), and neodecanoic acid (Versatic acid 10). Based on the pH isotherms, D2EHPA would be the most reasonable for Mn extraction and impurity removal. Cyanex 272 would be more suitable for Co separation than PC88A, and Versatic acid 10 is preferred for Cu extraction over other metals. In conclusion, the optimal combination of extractants is suggested for the recovery of valuable metals.
Conductive Zinc-Based Metal–Organic Framework Nanorods as Cathodes for High-Performance Zn-Ion Capacitors
Authors: Sun Jinfeng , Zhang Qian , Liu Chanjuan , Zhang Anning , Hou Linrui , Yuan Changzhou
Published: June 2024

Zinc-ion capacitors (ZICs), combining the merits of both high-energy zinc-ion batteries and high-power supercapacitors, are known as high-potential electrochemical energy storage (EES) devices. However, the research on ZICs still faces many challenges because of the lack of appropriate cathode materials with robust crystal structures and rich channels for stable and fast Zn2+ ion transport. In this study, we synthesized a robust, conductive, two-dimensional metal–organic framework (MOF) material, zinc-benzenehexathiolate (Zn-BHT), and investigated its electrochemical performance for zinc storage. Zn2+ ions could insert into/extricate from the host structure with a high diffusion rate, enabling the Zn-BHT cathode to exhibit a surface-controlled charge storage mechanism. Due to its unique structure, Zn-BHT exhibited a good reversible discharge capacity approaching 90.4 mAh g−1 at 0.1 A g−1, as well as a desirable rate capability and good cycling performance. In addition, a ZIC device was fabricated using the Zn-BHT cathode and a polyaniline-derived porous carbon (PC) anode, which depicted a high working voltage of up to 1.8 V and a high energy density of ~37.2 Wh kg−1. This work shows that conductive MOFs are high-potential electrode materials for ZICs and provide new enlightenment for the development of electrode materials for EES devices.