bluuebunny/crossref_metadata_2025 huggingface dataset:
Position-Dependent Influence of the Three Trp Residues on the Membrane Activity of the Antimicrobial Peptide, Tritrpticin
Authors: Arias Mauricio , Nguyen Leonard , Kuczynski Andrea , Lejon Tore , Vogel Hans
Published: November 2014

Antimicrobial peptides (AMPs) constitute promising candidates for the development of new antibiotics. Among the ever-expanding family of AMPs, tritrpticin has strong antimicrobial activity against a broad range of pathogens. This 13-residue peptide has an unusual amino acid sequence that is almost symmetrical and features three central Trp residues with two Arg residues near each end of the peptide. In this work, the role of the three sequential Trp residues in tritrpticin was studied in a systematic fashion by making a series of synthetic peptides with single-, double- and triple-Trp substitutions to Tyr or Ala. 1H NMR and fluorescence spectroscopy demonstrated the ability of all of the tritrpticin-analog peptides to interact with negatively-charged membranes. Consequently, most tritrpticin analogs exhibited the ability to permeabilize synthetic ePC:ePG (egg-yolk phosphatidylcholine (ePC), egg-yolk phosphatidylglycerol (ePG)) vesicles and live Escherichia coli bacteria. The membrane perturbation characteristics were highly dependent on the location of the Trp residue substitution, with Trp6 being the most important residue and Trp8 the least. The membrane permeabilization activity of the peptides in synthetic and biological membranes was directly correlated with the antimicrobial potency of the peptides against E. coli. These results contribute to the understanding of the role of each of the three Trp residues to the antimicrobial activity of tritrpticin.
Auranofin, an Anti-Rheumatic Gold Compound, Modulates Apoptosis by Elevating the Intracellular Calcium Concentration ([Ca2+]i) in MCF-7 Breast Cancer Cells
Authors: Varghese Elizabeth , Büsselberg Dietrich
Published: November 2014

Auranofin, a transition metal complex is used for the treatment of rheumatoid arthritis but is also an effective anti-cancer drug. We investigate the effects of Auranofin in inducing cell death by apoptosis and whether these changes are correlated to changes of intracellular calcium concentration ([Ca2+]i) in breast cancer cells (MCF-7). Cytotoxicity of Auranofin was evaluated using MTS assay and the Trypan blue dye exclusion method. With fluorescent dyes SR-FLICA and 7-AAD apoptotic death and necrotic death were differentiated by Flow cytometry. A concentration dependent decrease in the viability occurred and cells were shifted to the apoptotic phase. Intracellular calcium ([Ca2+]i) was recorded using florescence microscopy and a calcium sensitive dye (Fluo-4 AM) with a strong negative correlation (r = −0.713) to viability. Pharmacological modulators 2-APB (50 μM), Nimodipine (10 μM), Caffeine (10 mM), SKF 96365(20 μM) were used to modify calcium entry and release. Auranofin induced a sustained increase of [Ca2+]i in a concentration and time dependent manner. The use of different blockers of calcium channels did not reveal the source for the rise of [Ca2+]i. Overall, elevation of [Ca2+]i by Auranofin might be crucial for triggering Ca2+-dependent apoptotic pathways. Therefore, in anti-cancer therapy, modulating [Ca2+]i should be considered as a crucial factor for the induction of cell death in cancer cells.
To Which Degree Does Sector Specific Standardization Make Life Cycle Assessments Comparable?—The Case of Global Warming Potential of Smartphones
Authors: Andrae Anders , Vaija Mikko
Published: November 2014

Here attributional life cycle assessments (LCAs) for the same smartphone model are presented by two different organizations (Orange, OGE and Huawei, HuW) and the effect of different modeling approach is analyzed. A difference of around 32% (29.6 kg and 39.2 kg) for CO2e baseline scores is found using same study object and sector specific LCA standard, however, different metrics, emission intensities, and LCA software programs. The CO2e difference is reduced to 12% (29.9 kg and 33.5 kg) when OGE use HuW metrics for use phase power consumption and total mass, and when HuW use OGE metrics for gold mass and silicon die area. Further, a probability test confirms that present baseline climate change results, for one specific study object modeled with two largely different and independent LCA modeling approaches, are comparable if both use the European Telecommunications Standard Institute (ETSI) LCA standard. The general conclusion is that the ETSI LCA standard strongly facilitates comparable CC results for technically comparable smartphone models. Moreover, thanks to the reporting requirements of ETSI LCA standard, a clear understanding of the differences between LCA modeling approaches is obtained. The research also discusses the magnitude of the CO2e reduction potential in the life cycle of smartphones.
Plant Friendly Input Design for Parameter Estimation in an Inertial System with Respect to D-Efficiency Constraints
Authors: Jakowluk Wiktor
Published: November 2014

System identification, in practice, is carried out by perturbing processes or plants under operation. That is why in many industrial applications a plant-friendly input signal would be preferred for system identification. The goal of the study is to design the optimal input signal which is then employed in the identification experiment and to examine the relationships between the index of friendliness of this input signal and the accuracy of parameter estimation when the measured output signal is significantly affected by noise. In this case, the objective function was formulated through maximisation of the Fisher information matrix determinant (D-optimality) expressed in conventional Bolza form. As setting such conditions of the identification experiment we can only talk about the D-suboptimality, we quantify the plant trajectories using the D-efficiency measure. An additional constraint, imposed on D-efficiency of the solution, should allow one to attain the most adequate information content from the plant which operating point is perturbed in the least invasive (most friendly) way. A simple numerical example, which clearly demonstrates the idea presented in the paper, is included and discussed.
New Insights into the Fractional Order Diffusion Equation Using Entropy and Kurtosis
Authors: Ingo Carson , Magin Richard , Parrish Todd
Published: November 2014

Fractional order derivative operators offer a concise description to model multi-scale, heterogeneous and non-local systems. Specifically, in magnetic resonance imaging, there has been recent work to apply fractional order derivatives to model the non-Gaussian diffusion signal, which is ubiquitous in the movement of water protons within biological tissue. To provide a new perspective for establishing the utility of fractional order models, we apply entropy for the case of anomalous diffusion governed by a fractional order diffusion equation generalized in space and in time. This fractional order representation, in the form of the Mittag–Leffler function, gives an entropy minimum for the integer case of Gaussian diffusion and greater values of spectral entropy for non-integer values of the space and time derivatives. Furthermore, we consider kurtosis, defined as the normalized fourth moment, as another probabilistic description of the fractional time derivative. Finally, we demonstrate the implementation of anomalous diffusion, entropy and kurtosis measurements in diffusion weighted magnetic resonance imaging in the brain of a chronic ischemic stroke patient.
Learning about Drinking Water: How Important are the Three Dimensions of Knowledge that Can Change Individual Behavior?
Authors: Fremerey Christian , Bogner Franz
Published: November 2014

Clean drinking water, our most important resource, needs comprehensive protection. Due to its ubiquitous availability, the awareness of the importance of clean drinking water has partially vanished. Therefore, sensitizing within this context and improving individual ecological behavior has become an important issue in science curricula. We developed a student-centered guided-learning module based on nine workstations, with the themes: occurrence rates, purification methods, cleaning guidelines, distribution patterns, use and consumption, pollution, problems, types of drinking water and virtual water. One hundred and seventy four ninth to eleventh graders participated in our outreach intervention. All tasks presented via workstations were completed before participants inspected a nearby reed sewage treatment plant and completed hands-on experiments. For empirical analyses, we collected the newly acquired knowledge in three dimensions: system-knowledge, action-related knowledge and effectiveness knowledge, which together are assumed to provide a sufficient basis for conservation behavior. System knowledge directly affects action-related and effectiveness knowledge and these two types of knowledge, in turn, affect directly the ecological behavior. At all three test schedules, the three dimensions of knowledge correlated with each other, especially in both follow-up tests. The relevance of these results for schools is discussed.
Lorenz Wind Disturbance Model Based on Grey Generated Components
Authors: Zhang Yagang , Yang Jingyun , Wang Kangcheng , Wang Yinding
Published: November 2014

In order to meet the needs of wind speed prediction in wind farms, we consider the influence of random atmospheric disturbances on wind variations. Considering a simplified fluid convection mode, a Lorenz system can be employed as an atmospheric disturbance model. Here Lorenz disturbance is defined as the European norm of the solutions of the Lorenz equation. Grey generating and accumulated generating models are employed to explore the relationship between wind speed and its related disturbance series. We conclude that a linear or quadric polynomial generating model are optimal through the verification of short-term wind speed prediction in the Sotavento wind farm. The new proposed model not only greatly improves the precision of short-term wind speed prediction, but also has great significance for the maintenance and stability of wind power system operation.
Delivery of a Clinical Genomics Service
Authors: Newman William , Black Graeme
Published: November 2014

Over the past five years, next generation sequencing has revolutionised the discovery of genes responsible for rare inherited diseases previously resistant to traditional discovery techniques. This review considers how this new technology is being introduced into clinical practice to aid diagnosis and improve the clinical management of individuals and families affected by rare diseases where access to genetic testing was previously limited. We compare and contrast the different approaches that have been adopted including panel based tests, exome and genome sequencing. We provide insights from our own clinical practice demonstrating the challenges and benefits of this new technology.
Reasoning the Causality of City Sprawl, Traffic Congestion, and Green Land Disappearance in Taiwan Using the CLD Model
Authors: Chen Mei-Chih , Chang Kaowen
Published: November 2014

Many city governments choose to supply more developable land and transportation infrastructure with the hope of attracting people and businesses to their cities. However, like those in Taiwan, major cities worldwide suffer from traffic congestion. This study applies the system thinking logic of the causal loops diagram (CLD) model in the System Dynamics (SD) approach to analyze the issue of traffic congestion and other issues related to roads and land development in Taiwan’s cities. Comparing the characteristics of development trends with yearbook data for 2002 to 2013 for all of Taiwan’s cities, this study explores the developing phenomenon of unlimited city sprawl and identifies the cause and effect relationships in the characteristics of development trends in traffic congestion, high-density population aggregation in cities, land development, and green land disappearance resulting from city sprawl. This study provides conclusions for Taiwan’s cities’ sustainability and development (S&D). When developing S&D policies, during decision making processes concerning city planning and land use management, governments should think with a holistic view of carrying capacity with the assistance of system thinking to clarify the prejudices in favor of the unlimited developing phenomena resulting from city sprawl.
The Discovery of Aurora Kinase Inhibitor by Multi-Docking-Based Virtual Screening
Authors: Kim Jun-Tae , Jung Seo , Kang Sun , Ryu Chung-Kyu , Kang Nam
Published: November 2014

We report the discovery of aurora kinase inhibitor using the fragment-based virtual screening by multi-docking strategy. Among a number of fragments collected from eMololecules, we found four fragment molecules showing potent activity (>50% at 100 μM) against aurora kinase. Based on the explored fragment scaffold, we selected two compounds in our synthesized library and validated the biological activity against Aurora kinase.
An Uncleaved Signal Peptide Directs the Malus xiaojinensis Iron Transporter Protein Mx IRT1 into the ER for the PM Secretory Pathway
Authors: Zhang Peng , Tan Song , Berry James , Li Peng , Ren Na , Li Shuang , Yang Guang , Wang Wei-Bing , Qi Xiao-Ting , Yin Li-Ping
Published: November 2014

Malus xiaojinensis iron-regulated transporter 1 (Mx IRT1) is a highly effective inducible iron transporter in the iron efficient plant Malus xiaojinensis. As a multi-pass integral plasma membrane (PM) protein, Mx IRT1 is predicted to consist of eight transmembrane domains, with a putative N-terminal signal peptide (SP) of 1–29 amino acids. To explore the role of the putative SP, constructs expressing Mx IRT1 (with an intact SP) and Mx DsIRT1 (with a deleted SP) were prepared for expression in Arabidopsis and in yeast. Mx IRT1 could rescue the iron-deficiency phenotype of an Arabidopsis irt1 mutant, and complement the iron-limited growth defect of the yeast mutant DEY 1453 (fet3fet4). Furthermore, fluorescence analysis indicated that a chimeric Mx IRT1-eGFP (enhanced Green Fluorescent Protein) construct was translocated into the ER (Endoplasmic reticulum) for the PM sorting pathway. In contrast, the SP-deleted Mx DsIRT1 could not rescue either of the mutant phenotypes, nor direct transport of the GFP signal into the ER. Interestingly, immunoblot analysis indicated that the SP was not cleaved from the mature protein following transport into the ER. Taken together, data presented here provides strong evidence that an uncleaved SP determines ER-targeting of Mx IRT1 during the initial sorting stage, thereby enabling the subsequent transport and integration of this protein into the PM for its crucial role in iron uptake.
Long Non-Coding RNAs: Critical Players in Hepatocellular Carcinoma
Authors: Sun Jin , Bie Beibei , Zhang Shu , Yang Jun , Li Zongfang
Published: November 2014

Hepatocellular carcinoma (HCC) is a complex disease with multiple underlying pathogenic mechanisms caused by a variety of etiologic factors. Emerging evidence showed that long non-coding RNAs (lncRNAs), with size larger than 200 nucleotides (nt), play important roles in various types of cancer development and progression. In recent years, some dysregulated lncRNAs in HCC have been revealed and roles for several of them in HCC have been characterized. All these findings point to the potential of lncRNAs as prospective novel therapeutic targets in HCC. In this review, we summarize known dysregulated lncRNAs in HCC, and review potential biological roles and underlying molecular mechanisms of lncRNAs in HCC. Additionally, we discussed prospects of lncRNAs as potential biomarker and therapeutic target for HCC. In conclusion, this paper will help us gain better understanding of molecular mechanisms by which lncRNAs perform their function in HCC and also provide general strategies and directions for future research.
The Effects of NAD+ on Apoptotic Neuronal Death and Mitochondrial Biogenesis and Function after Glutamate Excitotoxicity
Authors: Wang Xiaowan , Li Hailong , Ding Shinghua
Published: November 2014

NAD+ is an essential co-enzyme for cellular energy metabolism and is also involved as a substrate for many cellular enzymatic reactions. It has been shown that NAD+ has a beneficial effect on neuronal survival and brain injury in in vitro and in vivo ischemic models. However, the effect of NAD+ on mitochondrial biogenesis and function in ischemia has not been well investigated. In the present study, we used an in vitro glutamate excitotoxicity model of primary cultured cortical neurons to study the effect of NAD+ on apoptotic neuronal death and mitochondrial biogenesis and function. Our results show that supplementation of NAD+ could effectively reduce apoptotic neuronal death, and apoptotic inducing factor translocation after neurons were challenged with excitotoxic glutamate stimulation. Using different approaches including confocal imaging, mitochondrial DNA measurement and Western blot analysis of PGC-1 and NRF-1, we also found that NAD+ could significantly attenuate glutamate-induced mitochondrial fragmentation and the impairment of mitochondrial biogenesis. Furthermore, NAD+ treatment effectively inhibited mitochondrial membrane potential depolarization and NADH redistribution after excitotoxic glutamate stimulation. Taken together, our results demonstrated that NAD+ is capable of inhibiting apoptotic neuronal death after glutamate excitotoxicity via preserving mitochondrial biogenesis and integrity. Our findings provide insights into potential neuroprotective strategies in ischemic stroke.
High-Energy-Low-Temperature Technologies for the Synthesis of Nanoparticles: Microwaves and High Pressure
Authors: Lojkowski Witold , Leonelli Cristina , Chudoba Tadeusz , Wojnarowicz Jacek , Majcher Andrzej , Mazurkiewicz Adam
Published: November 2014

Microwave Solvothermal Synthesis (MSS) is a chemical technology, where apart from possible effects of microwaves on the chemical reaction paths, microwave heating allows the precise planning of a time-temperature schedule, as well as to achieve high super-saturation of the reagents uniformly in the reactor vessel. Thus, MSS is suitable for production of nanoparticles with small grain size distribution and a high degree of crystallinity. A further advantage of the technology is a much lower synthesis temperature than for gas phase, plasma or sol-gel technologies. New reactors have been developed to exploit these advantages of the MSS technology of nanoparticles synthesis and to scale up the production rate. Reactor design and realization has been shown to be decisive and critical for the control of the MSS technology. Examples of oxidic and phosphatic nanoparticles synthesis have been reported.
Microgravity-Induced Fluid Shift and Ophthalmic Changes
Authors: Nelson Emily , Mulugeta Lealem , Myers Jerry
Published: November 2014

Although changes to visual acuity in spaceflight have been observed in some astronauts since the early days of the space program, the impact to the crew was considered minor. Since that time, missions to the International Space Station have extended the typical duration of time spent in microgravity from a few days or weeks to many months. This has been accompanied by the emergence of a variety of ophthalmic pathologies in a significant proportion of long-duration crewmembers, including globe flattening, choroidal folding, optic disc edema, and optic nerve kinking, among others. The clinical findings of affected astronauts are reminiscent of terrestrial pathologies such as idiopathic intracranial hypertension that are characterized by high intracranial pressure. As a result, NASA has placed an emphasis on determining the relevant factors and their interactions that are responsible for detrimental ophthalmic response to space. This article will describe the Visual Impairment and Intracranial Pressure syndrome, link it to key factors in physiological adaptation to the microgravity environment, particularly a cephalad shifting of bodily fluids, and discuss the implications for ocular biomechanics and physiological function in long-duration spaceflight.
Function and Regulation of Ferredoxins in the Cyanobacterium, Synechocystis PCC6803: Recent Advances
Authors: Cassier-Chauvat Corinne , Chauvat Franck
Published: November 2014

Ferredoxins (Fed), occurring in most organisms, are small proteins that use their iron-sulfur cluster to distribute electrons to various metabolic pathways, likely including hydrogen production. Here, we summarize the current knowledge on ferredoxins in cyanobacteria, the prokaryotes regarded as important producers of the oxygenic atmosphere and biomass for the food chain, as well as promising cell factories for biofuel production. Most studies of ferredoxins were performed in the model strain, Synechocystis PCC6803, which possesses nine highly-conserved ferredoxins encoded by monocistronic or operonic genes, some of which are localized in conserved genome regions. Fed1, encoded by a light-inducible gene, is a highly abundant protein essential to photosynthesis. Fed2-Fed9, encoded by genes differently regulated by trophic conditions, are low-abundant proteins that play prominent roles in the tolerance to environmental stresses. Concerning the selectivity/redundancy of ferredoxin, we report that Fed1, Fed7 and Fed9 belong to ferredoxin-glutaredoxin-thioredoxin crosstalk pathways operating in the protection against oxidative and metal stresses. Furthermore, Fed7 specifically interacts with a DnaJ-like protein, an interaction that has been conserved in photosynthetic eukaryotes in the form of a composite protein comprising DnaJ- and Fed7-like domains. Fed9 specifically interacts with the Flv3 flavodiiron protein acting in the photoreduction of O2 to H2O.
The Role of the Clathrin Adaptor AP-1: Polarized Sorting and Beyond
Authors: Nakatsu Fubito , Hase Koji , Ohno Hiroshi
Published: November 2014

The selective transport of proteins or lipids by vesicular transport is a fundamental process supporting cellular physiology. The budding process involves cargo sorting and vesicle formation at the donor membrane and constitutes an important process in vesicular transport. This process is particularly important for the polarized sorting in epithelial cells, in which the cargo molecules need to be selectively sorted and transported to two distinct destinations, the apical or basolateral plasma membrane. Adaptor protein (AP)-1, a member of the AP complex family, which includes the ubiquitously expressed AP-1A and the epithelium-specific AP-1B, regulates polarized sorting at the trans-Golgi network and/or at the recycling endosomes. A growing body of evidence, especially from studies using model organisms and animals, demonstrates that the AP-1-mediated polarized sorting supports the development and physiology of multi-cellular units as functional organs and tissues (e.g., cell fate determination, inflammation and gut immune homeostasis). Furthermore, a possible involvement of AP-1B in the pathogenesis of human diseases, such as Crohn’s disease and cancer, is now becoming evident. These data highlight the significant contribution of AP-1 complexes to the physiology of multicellular organisms, as master regulators of polarized sorting in epithelial cells.
Design of a Nickel-Based Bond-Coat Alloy for Thermal Barrier Coatings on Copper Substrates
Authors: Fiedler Torben , Fedorova Tatiana , Rösler Joachim , Bäker Martin
Published: November 2014

To increase the lifetime of rocket combustion chambers, thermal barrier coatings (TBC) may be applied on the copper chamber wall. Since standard TBC systems used in gas turbines are not suitable for rocket-engine application and fail at the interface between the substrate and bond coat, a new bond-coat material has to be designed. This bond-coat material has to be chemically compatible to the copper substrate to improve the adhesion and needs a coefficient of thermal expansion close to that of copper to reduce thermal stresses. One approach to achieve this is to modify the standard NiCrAlY alloy used in gas turbines by adding copper. In this work, the influence of copper on the microstructure of NiCrAlY-alloys is investigated with thermodynamical calculations, optical microscopy, SEM, EDX and calorimetry. Adding copper leads to the formation of a significant amount of \(\beta\) and \(\alpha\) Reducing the aluminum and chromium content leads furthermore to a two-phase fcc microstructure.
Biochemical Validation of the Older Australian’s Food Frequency Questionnaire Using Carotenoids and Vitamin E
Authors: Lai Jun , Attia John , McEvoy Mark , Hure Alexis
Published: November 2014

Background: Validation of a food frequency questionnaire (FFQ) is important, as inaccurate and imprecise information may affect the association between dietary exposure and health outcomes. Objective: This study assessed the validity of the Older Australian’s FFQ against plasma carotenoids and Vitamin E. Methods: A random subsample (n = 150) of 2420 participants in the Hunter Community Study, aged 55–85 years, were included. Correlations between crude and energy-adjusted FFQ estimates of carotenoids, Vitamin E, and fruit and vegetables with corresponding biomarkers were determined. Percentages of participants correctly classified in the same quartile, and in the same ± 1 quartile, by the two methods were calculated. Results: Significant correlations (P < 0.05) were observed for α-carotene (r = 0.26–0.28), β-carotene (r = 0.21–0.25), and β-cryptoxanthin (r = 0.21–0.23). Intakes of fruits and vegetables also showed similar correlations with these plasma carotenoids. Lycopene was only significantly correlated with fruit and vegetable intakes (r = 0.19–0.23). Weak correlations were observed for lutein + zeaxanthin (r = 0.12–0.16). For Vitamin E, significant correlation was observed for energy-adjusted FFQ estimate and biomarker (r = 0.20). More than 68% of individuals were correctly classified within the same or adjacent quartile, except for lutein + zeaxanthin. Conclusion: With the exception of lutein + zeaxanthin, the Older Australian’s FFQ provides reasonable rankings for individuals according to their carotenoids, Vitamin E, fruit and vegetable intakes.
Quantifying the Effects of Photoperiod, Temperature and Daily Irradiance on Flowering Time of Soybean Isolines
Authors: Cober Elroy , Curtis Daniel , Stewart Douglas , Morrison Malcolm
Published: November 2014

Soybean isolines with different combinations of photoperiod sensitivity alleles were planted in a greenhouse at different times during the year resulting in natural variation in daily incident irradiance and duration. The time from planting to first flower were observed. Mathematical models, using additive and multiplicative modes, were developed to quantify the effect of photoperiod, temperature, photoperiod-temperature interactions, rate of photoperiod change, and daily solar irradiance on flowering time. Observed flowering times correlated with predicted times (R2 = 0.92, Standard Error of the Estimate (SSE) = 2.84 d, multiplicative mode; R2 = 0.91, SSE = 2.88 d, additive mode). The addition of a rate of photoperiod change function and an irradiance function to the temperature and photoperiod functions improved the accuracy of flowering time prediction. The addition of a modified photoperiod function, which allowed for photoperiod sensitivity at shorter photoperiods, improved prediction of flowering time. Both increasing and decreasing rate of photoperiod change, as well as low levels of daily irradiance delayed flowering in soybean. The complete model, which included terms for the rate of photoperiod change, photoperiod, temperature and irradiance, predicted time to first flower in soybean across a range of environmental conditions with an SEE of 3.6 days when tested with independent data.
Object-Based Analysis of Airborne LiDAR Data for Building Change Detection
Authors: Pang Shiyan , Hu Xiangyun , Wang Zizheng , Lu Yihui
Published: November 2014

Building change detection is useful for land management, disaster assessment, illegal building identification, urban growth monitoring, and geographic information database updating. This study proposes an automatic method that applies object-based analysis to multi-temporal point cloud data to detect building changes. The aim of this building change detection method is to identify areas that have changed and to obtain from-to information. In this method, the data are first preprocessed to generate two sets of digital surface models (DSMs), digital elevation models, and normalized DSMs from registered old and new point cloud data. Thereafter, on the basis of differential DSM, candidates for changed building objects are identified from the points in the smooth areas by using a connected component analysis technique. The random sample consensus fitting algorithm is then used to distinguish the true changed buildings from trees. The changed building objects are classified as “newly built”, “taller”, “demolished” or “lower” by using rule-based analysis. Finally, a test data set consisting of many buildings of different types in an 8.5 km2 area is selected for the experiment. In the test data set, the method correctly detects 97.8% of buildings larger than 50 m2. The accuracy of the method is 91.2%. Furthermore, to decrease the workload of subsequent manual checking of the result, the confidence index for each changed object is computed on the basis of object features.
Estimation of Airborne Lidar-Derived Tropical Forest Canopy Height Using Landsat Time Series in Cambodia
Authors: Ota Tetsuji , Ahmed Oumer , Franklin Steven , Wulder Michael , Kajisa Tsuyoshi , Mizoue Nobuya , Yoshida Shigejiro , Takao Gen , Hirata Yasumasa , Furuya Naoyuki , Sano Takio , Heng Sokh , Vuthy Ma
Published: November 2014

In this study, we test and demonstrate the utility of disturbance and recovery information derived from annual Landsat time series to predict current forest vertical structure (as compared to the more common approaches, that consider a sample of airborne Lidar and single-date Landsat derived variables). Mean Canopy Height (MCH) was estimated separately using single date, time series, and the combination of single date and time series variables in multiple regression and random forest (RF) models. The combination of single date and time series variables, which integrate disturbance history over the entire time series, overall provided better MCH prediction than using either of the two sets of variables separately. In general, the RF models resulted in improved performance in all estimates over those using multiple regression. The lowest validation error was obtained using Landsat time series variables in a RF model (R2 = 0.75 and RMSE = 2.81 m). Combining single date and time series data was more effective when the RF model was used (opposed to multiple regression). The RMSE for RF mean canopy height prediction was reduced by 13.5% when combining the two sets of variables as compared to the 3.6% RMSE decline presented by multiple regression. This study demonstrates the value of airborne Lidar and long term Landsat observations to generate estimates of forest canopy height using the random forest algorithm.
The Potential and Uptake of Remote Sensing in Insurance: A Review
Authors: De Leeuw Jan , Vrieling Anton , Shee Apurba , Atzberger Clement , Hadgu Kiros , Biradar Chandrashekhar , Keah Humphrey , Turvey Calum
Published: November 2014

Global insurance markets are vast and diverse, and may offer many opportunities for remote sensing. To date, however, few operational applications of remote sensing for insurance exist. Papers claiming potential application of remote sensing typically stress the technical possibilities, without considering its contribution to customer value for the insured or to the profitability of the insurance industry. Based on a systematic search of available literature, this review investigates the potential and actual support of remote sensing to the insurance industry. The review reveals that research on remote sensing in classical claim-based insurance described in the literature revolve around crop damage and flood and fire risk assessment. Surprisingly, the use of remote sensing in claim-based insurance appears to be instigated by government rather than the insurance industry. In contrast, insurance companies are offering various index insurance products that are based on remote sensing. For example, remotely sensed index insurance for rangelands and livestock are operational, while various applications in crop index insurance are being considered or under development. The paper discusses these differences and concludes that there is particular scope for application of remote sensing by the insurance industry in index insurance because (1) indices can be constructed that correlate well with what is insured; (2) these indices can be delivered at low cost; and (3) it opens up new markets that are not served by claim-based insurance. The paper finally suggests that limited adoption of remote sensing in insurance results from a lack of mutual understanding and calls for greater cooperation between the insurance industry and the remote sensing community.
Numerical Study of Urban Canyon Microclimate Related to Geometrical Parameters
Authors: De Lieto Vollaro Andrea , De Simone Giuseppe , Romagnoli Roberto , Vallati Andrea , Botillo Simone
Published: November 2014

In this study a microclimate analysis on a particular urban configuration: the—street canyon—has been carried out. The analysis, conducted by performing numerical simulations using the finite volumes commercial code ANSYS-Fluent, shows the flow field in an urban environment, taking into account three different aspect ratios (H/W). This analysis can be helpful in the study on urban microclimate and on the heat exchanges with the buildings. Fluid-dynamic fields on vertical planes within the canyon, have been evaluated. The results show the importance of the geometrical configuration, in relation to the ratio between the height (H) of the buildings and the width (W) of the road. This is a very important subject from the point of view of “Smart Cities”, considering the urban canyon as a subsystem of a larger one (the city), which is affected by climate changes.
Rapid Analysis of Deoxynivalenol in Durum Wheat by FT-NIR Spectroscopy
Authors: De Girolamo Annalisa , Cervellieri Salvatore , Visconti Angelo , Pascale Michelangelo
Published: November 2014

Fourier-transform-near infrared (FT-NIR) spectroscopy has been used to develop quantitative and classification models for the prediction of deoxynivalenol (DON) levels in durum wheat samples. Partial least-squares (PLS) regression analysis was used to determine DON in wheat samples in the range of <50–16,000 µg/kg DON. The model displayed a large root mean square error of prediction value (1,977 µg/kg) as compared to the EU maximum limit for DON in unprocessed durum wheat (i.e., 1,750 µg/kg), thus making the PLS approach unsuitable for quantitative prediction of DON in durum wheat. Linear discriminant analysis (LDA) was successfully used to differentiate wheat samples based on their DON content. A first approach used LDA to group wheat samples into three classes: A (DON ≤ 1,000 µg/kg), B (1,000 < DON ≤ 2,500 µg/kg), and C (DON > 2,500 µg/kg) (LDA I). A second approach was used to discriminate highly contaminated wheat samples based on three different cut-off limits, namely 1,000 (LDA II), 1,200 (LDA III) and 1,400 µg/kg DON (LDA IV). The overall classification and false compliant rates for the three models were 75%–90% and 3%–7%, respectively, with model LDA IV using a cut-off of 1,400 µg/kg fulfilling the requirement of the European official guidelines for screening methods. These findings confirmed the suitability of FT-NIR to screen a large number of wheat samples for DON contamination and to verify the compliance with EU regulation.
Molecular and Insecticidal Characterization of a Novel Cry-Related Protein from Bacillus Thuringiensis Toxic against Myzus persicae
Authors: Palma Leopoldo , Muñoz Delia , Berry Colin , Murillo Jesús , De Escudero Iñigo , Caballero Primitivo
Published: November 2014

This study describes the insecticidal activity of a novel Bacillus thuringiensis Cry-related protein with a deduced 799 amino acid sequence (~89 kDa) and ~19% pairwise identity to the 95-kDa-aphidicidal protein (sequence number 204) from patent US 8318900 and ~40% pairwise identity to the cancer cell killing Cry proteins (parasporins Cry41Ab1 and Cry41Aa1), respectively. This novel Cry-related protein contained the five conserved amino acid blocks and the three conserved domains commonly found in 3-domain Cry proteins. The protein exhibited toxic activity against the green peach aphid, Myzus persicae (Sulzer) (Homoptera: Aphididae) with the lowest mean lethal concentration (LC50 = 32.7 μg/mL) reported to date for a given Cry protein and this insect species, whereas it had no lethal toxicity against the Lepidoptera of the family Noctuidae Helicoverpa armigera (Hübner), Mamestra brassicae (L.), Spodoptera exigua (Hübner), S. frugiperda (J.E. Smith) and S. littoralis (Boisduval), at concentrations as high as ~3.5 μg/cm2. This novel Cry-related protein may become a promising environmentally friendly tool for the biological control of M. persicae and possibly also for other sap sucking insect pests.
Separating the Impacts of Climate Variation and Human Activities on Runoff in the Songhua River Basin, Northeast China
Authors: Li Fengping , Zhang Guangxin , Xu Yi
Published: November 2014

Climate variation and human activities are commonly recognized as two major factors affecting basin hydrology. However, quantifying their individual effect on runoff is challenging. In this study, long-term (1960–2009) river discharge and weather data in the Songhua River Basin (SRB, 556,800 km2), Northeast China, were gathered to separate the impacts of climate variation and human activities on runoff in five sub basins of the SRB. Mann-Kendall test, moving t-test and precipitation-runoff double cumulative curve were utilized to identify trends and change points of the hydrometeorlogical variables. Based on the change point, the 50-year study period was divided into two time series: 1960–1974 where minimal human activities took place and 1975–2009 where extensive land use change occurred and river engineering projects were undertaken. Subsequently, individual contributions of climate and human factors were assessed through a hydrologic sensitivity analysis. Our study found a significant decline in runoff of the SRB over the past 50 years. Contribution of climate variation and human activities to the change varied temporally and spatially. For the 1975–2009 period, human activities made a greater contribution (62%–82%) to the total runoff decline of the SRB. However, climate variation played a bigger role in runoff reduction in two sub river basins (63%–65%) between 1975 and 1989, as well as in runoff increase in other two sub river basins (85%–86%) between 1990 and 1999. Spatially, the effect of human activities on runoff decline was relatively stronger in the lower basin areas in the 1960s and 1970s while showing an increasing role in the upper basin areas in the past two decades.
Géomorphologie, géoarchéologie et géographie en pays dogon : vers une reconstitution du passé climatique et du peuplement du Sahel malien à partir du site d'Ounjougou. (Geomorphology, geoarchaeology and geography in Dogon country : palaeoenvironments and societies at Ounjougou.)
Authors: Rasse Michel
Published: November 2014

Reconstituting the environments in Western Africa, after paleoclimatic variations and the influence of man, is always a problem. In Ounjougou, studies carried out over the past few years, have shown that locally, conditions may have been exceptionally favorable to the conservation and the revelation of a very rich stratigraphic sequence. Many onsite missions, laboratory analyses and numerous absolute datings make it possible to propose a reconstitution of the major stages of the hydro sedimentary and paleoenvironmental evolution of the Yamé valley. The formations also make it possible to have an initial idea of the main climatic and anthropic modifications on the ecosystems. Everything indicates that man increased his action, probably as of the Middle Holocene, but above all during the Recent and Final Holocene.
Corte et ses relations avec les montagnes du centre de la Corse ( Corte and the mountains of Middle Corsica)
Authors: Benso Gérard
Published: November 2014

Les montagnes autour de Corte sont un espace où deux activités cohabitent et sont en partie complémentaires : le pastoralisme est ancestral, il domine encore nettement malgré un recul relatif, le tourisme est récent, il progresse lentement mais reste modeste. Ces activités se pratiquent dans un environnement remarquable dont les populations prennent conscience qu 'il doit être protégé. Corte est le seul centre urbain de la région ; c 'est une petite ville à dominante tertiaire rajeunie depuis un quart de siècle par l'implantation d'une université. Ce facteur va t' il modifier ses relations avec la montagne ?
Environnement montagnard, urbanisme et sensibilité écologique : le cas de Fribourg-en-Brisgau (Allemagne) (Mountain environment, urban policy and ecological awareness : the case of Freiburg (Germany))
Authors: Michna Rudolf
Published: November 2014

Au cours du XXe siècle, les rapports de Fribourg avec l'environnement montagnard ont changé de nature, d'intensité et de sens. La ville a fortement poussé en direction de la plaine rhénane. Des vieilles industries, fondamentalement xylophages, il ne subsiste que quelques rares entreprises. Pourtant l'environnement montagnard reste indirectement un facteur important de son essor engagé à partir de la fin du XIXe siècle. Le respect de son cadre naturel, cher au milieu bourgeois dès le XIXe siècle, s'est combiné à l'heure actuelle à une forte conscience écologique qui est particulièrement ancrée dans le milieu des nouveaux résidents, aboutissant à une politique urbaine orientée sur les modes de transport en commun, la promotion d'énergies renouvelables et la construction de quartiers 'durables '.
Les pratiques citadines d'un héros de roman policier (The urban practice of a hero of a detective novel)
Authors: Rosemberg Muriel
Published: November 2014

Dans Total Kheops, roman de J.-C. Izzo, la ville que parcourt et décrit le héros est plus que le simple décor d'une enquête policière, elle est l'objet de sa quête, le personnage central du récit. En montrant ce qu'est la ville par la façon dont elle est pratiquée, l'écrivain met en scène une expérience spatiale qui, à certains égards, peut être considérée comme un modèle de pratique citadine
Intégration fonctionnelle et marginalisation sociale en Plaine de France (Functionnal integration and social marginalization in Plaine de France)
Authors: Lebeau Boris
Published: November 2014

Des anciennes banlieues ouvrières caractérisées par la superposition, en un même lieu, d'une périphérie productive et d'une périphérie sociale, il ne subsiste plus grand-chose. Alors que ces espaces sont progressivement intégrés sur le plan économique et fonctionnel, en raison de leur relative proximité du centre, ils se retrouvent souvent fortement marginalisés sur le plan social. Cette dissociation suggère donc qu 'il n'y a plus nécessairement concordance entre les différentes formes de marginalité. Nous l 'illustrerons ici à partir du cas de la Plaine de France (Val d'Oise et Seine-Saint Denis).
La marginalisation des communautés de seniors en France et au Portugal (The marginalization of the senior communities in France and Portugal)
Authors: Bésingrand Didier
Published: November 2014

The last decade has seen the appearance of gated communities with residents of a specific age group, especially in France and Portugal. The differences between the two gated communities are noticeable regarding the nationality of the owners of a dwelling, their social origin and the degree of isolation of these communities. These phenomenas generate a spatial and social marginalization intended by the residents to cut themselves off the adjoining urban space. That chosen isolation raises the issue of the social mix, not only concerning the diversity of the age groups, but also the social categories.
Marginalité de l'Etat et violences sociales au Far Est (Tchad oriental) (Social violence in the margins of the state (eastern Chad))
Authors: Favre Johanne
Published: November 2014

Wadai is on the fringe of contemporary Chad. It is plagued by social conflicts which are the result of concurrent factors. Three episodes of murderous violence are examined to show the deciding part the state plays in perpetuating insecurity.
Secure Secondary Use of Clinical Data with Cloud-based NLP Services
Authors: Christoph J. , Griebel L. , Leb I. , Engel I. , Köpcke F. , Toddenroth D. , Prokosch H. -U. , Laufer J. , Marquardt K. , Sedlmayr M.
Published: November 2014

Objectives: The secondary use of clinical data provides large opportunities for clinical and translational research as well as quality assurance projects. For such purposes, it is necessary to provide a flexible and scalable infrastructure that is compliant with privacy requirements. The major goals of the cloud4health project are to define such an architecture, to implement a technical prototype that fulfills these requirements and to evaluate it with three use cases.

Methods: The architecture provides components for multiple data provider sites such as hospitals to extract free text as well as structured data from local sources and de-identify such data for further anonymous or pseudonymous processing. Free text documentation is analyzed and transformed into structured information by text-mining services, which are provided within a cloud-computing environment. Thus, newly gained annotations can be integrated along with the already available structured data items and the resulting data sets can be uploaded to a central study portal for further analysis.

Results: Based on the architecture design, a prototype has been implemented and is under evaluation in three clinical use cases. Data from several hundred patients provided by a University Hospital and a private hospital chain have already been processed.

Conclusions: Cloud4health has shown how existing components for secondary use of structured data can be complemented with text-mining in a privacy compliant manner. The cloud-computing paradigm allows a flexible and dynamically adaptable service provision that facilitates the adoption of services by data providers without own investments in respective hardware resources and software tools.
Effects of surface functionalization on the adsorption of human serum albumin onto nanoparticles – a fluorescence correlation spectroscopy study
Authors: Maffre Pauline , Brandholt Stefan , Nienhaus Karin , Shang Li , Parak Wolfgang J , Nienhaus G Ulrich
Published: November 2014

By using fluorescence correlation spectroscopy (FCS), we have studied the adsorption of human serum albumin (HSA) onto Fe–Pt nanoparticles (NPs, 6 nm radius), CdSe/ZnS quantum dots (QDs, 5 nm radius) and Au and Ag nanoclusters (1–4 nm radius), which are enshrouded by various water-solubilizing surface layers exposing different chemical functional groups (carboxyl, amino and both), thereby endowing the NPs with different surface charges. We have also measured the effects of modified surface functionalizations on the protein via succinylation and amination. A step-wise increase in hydrodynamic radius with protein concentration was always observed, revealing formation of protein monolayers coating the NPs, independent of their surface charge. The differences in the thickness of the protein corona were rationalized in terms of the different orientations in which HSA adsorbs onto the NPs. The midpoints of the binding transition, which quantifies the affinity of HSA toward the NP, were observed to differ by almost four orders of magnitude. These variations can be understood in terms of specific Coulombic interactions between the proteins and the NP surfaces.