bluuebunny/crossref_metadata_2025 huggingface dataset:
Pengaruh Terapi Bermain Flashcard Terhadap Interaksi Sosial pada Anak Autis di Yayasan Pendidikan Melati Ceria di Kota Palangka Raya
Authors: Safitri Neneng , Arsesiana Angga , Agustina Vina , Mawarni Risti
Published: February 2024

Anak berkebutuhan khusus (ABK) memiliki berbagai hambatan gangguan kelainan yang diakibatkan dari berbagai faktor penyebab, diantaranya adalah dikarenakan adanya kerusakan bentuk/kondisi organ mata, telinga, fisik/tubuh yang berpengaruh pada gerak, mental emosional dan sosial sehingga mempengaruhi dalam aktifitas kehidupan sehari-hari. Autis merupakan gangguan perkembangan neurobiologis yang sangat komplek/berat dalam kehidupan yang panjang, yang meliputi gangguan aspek interaksi sosial, komunikasi dan bahasa serta gangguan emosi dan persepsi sensori bahkan pada aspek motoriknya. Berdasarkan fenomena didapatkan ada beberapa anak autis yang berada di Yayasan Pendidikan Melati Ceria Kota Palangka Raya mengalami gangguan interaksi sosial. Hal ini terjadi karena anak tidak bersosialisasi dengan teman sebayanya. Penelitian ini bertujuan untuk menganalisis pengaruh terapi bermain terhadap interaksi sosial pada anak autis di Yayasan Pendidikan Melati Ceria Kota Palangka Raya. Metode Penelitian ini menggunakan metode  pre eksperiment dengan pendekatan one group pre post test design. Populasi berjumlah 15 responden. Sampel berjumlah 12 responden dengan  teknik sampling yang digunakan yaitu total sampling. Pengumpulan data menggunakan lembar observasi, kemudian data dianalisa menggunakan uji wilcoxon. Hasil analisa pengaruh terapi bermain flashcard terhadap interaksi sosial pada anak autis di Yayasan Pendidikan Melati Ceria Kota Palangka Raya, interaksi sosial pre dan post didapatkan signifikansi diperoleh sig. (2-tailed) 0.001 yang berarti lebih kecil dari 0.05, sehingga dapat disimpulkan bahwa p value <α (0.001< 0.05) maka H0 ditolak dan H1 diterima, artinya adanya pengaruh terapi bermain flashcard terhadap interaksi sosial. Diharapkan kepada guru sekolah luar biasa (SLB) dan petugas kesehatan dapat memberikan terapi bermain flashcard kepada anak autis sehingga dapat meningkatkan interaksi sosial mereka.
ОСОБЛИВОСТІ ПОСТАЧАННЯ ДЕРЖАВАМИ–ПАРТНЕРАМИ БРОНЕТАНКОВОГО ОЗБРОЄННЯ ТА ЙОГО ЕКСПЛУАТАЦІЇ У БОЙОВИХ УМОВАХ СИЛАМИ ОБОРОНИ УКРАЇНИ (2022–2023 рр.)
Authors: КРЕТ Микола
Published: February 2024

У статті досліджено процес становлення та розвитку системи міжнародної військово-тех-нічної допомоги держав-партнерів Силам оборони України. Визначено, що надходження зразківОВТ у рамках міжнародної військово-технічної допомоги дало змогу збільшити бойовий потенціалСил оборони України та ефективно протистояти повномасштабній російській агресії. На осно-ві інформації, отриманої від безпосередніх користувачів, виявлені переваги та недоліки сторо-ни зразків бронетанкового озброєння, що дає можливість надати рекомендації щодо підвищенняефективності їх бойового застосування.
Workplace learning during the COVID-19 epidemic and digital transition
Authors: Kersánszki Tamás , Fazekas Ágnes , Fazekas Nóra
Published: February 2024

Work experience is becoming an increasingly important part of human capital, so in this lifelong learning, organisations must encourage employees to perform better by providing career opportunities and multidimensional mobility. The shock effect caused by the pandemic and the changed circumstances had a noticeable impact on the organisations, which reacted to the changed environment sometimes with resistance and sometimes with adaptation (for example, collective learning and flexibility), the result of which were the various productive and innovative responses of the organisation to the changes (Spicer, 2020). As a result of the epidemic, millions of employees switched from one day to remote work, which has become a globally recognised form of work and the subject of research. Such a dynamic change in the digital transition has significantly impacted the workplace environment and learning and has changed attitudes about work, employment and workplace learning.
The homeschoolers inclusion in Higher Education. Szekler case study
Authors: Mandel Kinga
Published: February 2024

Abstract
 
The purpose of this paper is to study the higher education inclusion experiences of Szekler homeschooled youngsters. The main research question was how the homeschooled youngsters are entering the higher education institution, whether they have free access to all of them or just to some, how they are fitting in, socializing and performing during their studies. The small-scale thematic interview based non-representative quantitative research reached 7 homeschooling families raising 25 children, 13 persons, parents and youngsters with snowball sampling. Our main finding is that against all the inclusion concerns enlisted in the literature and in commons sense it seems that the Szekler homeschooled inclusion in higher education is in most of the cases same as their mates. They are facing same challenges and dealing with those similarly, in some cases even better, then their peers. It could be that this is due of the advantages of homeschooling, like self-awareness, self-reliance, independent learning skills, project work-, and foreign language skills, voluntary- and other practical life experiences. Our research results could contribute to deconstruct some stereotypes on homeschooling.
 
Key words: homeschooling, inclusion in higher education, Szeklerland, case study.
A tehetség-paradigma felülvizsgálata a szakképzésben
Authors: Suhajda Csilla Judit , Kovács Mónika
Published: February 2024

Hazánkban a szakképzésben a tehetséggondozás kevésbé hangsúlyos terület, mint a köznevelés egyéb színterein (OFI, 2015), és elsősorban a versenyfelkészítésben determinálódik. Az iskolák tehetségképe a klasszikus megnyilvánulási formákra fókuszál (Gyarmathy, 2015), miközben számos területen a potenciálból nem lesz produktum azon katalizátorok hiányában, amelyek a kimagasló teljesítmény képességeken felüli, intraperszonális területeit céloznák.
Kutatásunkban a szakképzésben megvalósuló tehetséggondozás hazai gyakorlatát vizsgáljuk: hogyan gondolkodnak az intézmények és a pedagógusok a tehetséggondozás helyéről, szerepéről a mindennapi oktatási-képzési feladatokban. Célunk az, hogy feltérképezzük, hogy az egyes szakképző intézmények, tanárok milyen eszközökkel, módszerekkel tudják támogatni a tehetségek kibontakozását, milyen jó gyakorlatok, technikák léteznek ezen a területeken. Kutatásunk eredményeit pedagógusok körében végzett kérdőíves vizsgálatunk és a minősített tehetséggondozó műhelyek programjainak elemzése biztosítják. Eredményeink rámutatnak arra, hogy a tehetség-paradigma felülvizsgálata a szakképzésben hozzájárulhat a nemzeti tehetségvagyon megóvásához.
Erasmus projektek a Nemzeti Szakképzési és Felnőttképzési Hivatalban
Authors: OpEE Szerkesztőség
Published: February 2024

2015 óta a Nemzeti Szakképzési és Felnőttképzési Hivatal nemzeti koordinációs feladatokat lát elszakképzés-fejlesztés, a pályaorientációs tanácsadás, és felnőttkori tanulás terén, illetve 2005 ótahozzájárul a hazai szakképesítések külföldi elismertetéséhez. A feladatok az Erasmus+ európai oktatásés képzés támogatására irányuló uniós programjainak keretében valósulnak meg. A 2021–2027-esprogramozási időszak nagy hangsúlyt fektet a társadalmi befogadásra, a zöld és digitális átállásra,valamint a fiatalok támogatására.
Lead-Free Conductors with a Surface Treatment Ensuring a Low Melting Temperature
Authors: Mareška Aleš , Kordová Tereza , Havlík Míka Martin
Published: February 2024

In order to protect the environment and to ensure sustainability, it is prohibited to use lead materials in the production of automotive components. As part of soldering, busbars are used that usually have a copper core and a coating made of tin, but the melting point of such material is high, which poses a problem during soldering. This article discusses the case where a busbar is interconnected with another conductor in a sealable pressure vessel where high temperatures and pressures are reached. The product is heated to a certain temperature and the melting temperature of the given bus coating is exceeded, resulting in soldering and an ideal connection of the wires. The problem is that the temperatures reached by the processor during this operation are lower than the melting temperatures of the busbar with a tin coating. In the article, the authors focus on the production and development of new types of busbars with a coating consisting of tin, bismuth and indium and discuss possible formulations and their effect on the melting temperature of the busbars.
The Nature of Vacuum Energy
Authors:   Free Researcher, Grenoble, France, Raverdy Yvan-Claude
Published: February 2024

The aim here is to show that the consideration of a quantum fluid, therefore granular, to describe space empty of matter and
particles, is likely to resolve the question of its energy density in agreement with the data of standard cosmology. The Casimir
effect should then make it possible to access its value which can be compared to the local gravitational energy. This also
explains why quantum field theory is ineffective in accounting for this quantity.
We also show that we can, through this basic hypothesis, give a concrete meaning to “dark energy” and “dark matter”.
Conocimientos en fotoprotección en taxista de la ciudad de Riobamba
Authors: Quishpi Lucero Verónica Cecilia , Caibe Abril María Belén , Cárdenas Cevallos Micaela Lisette , Guamán Román Josselyn Cristina
Published: February 2024

Introducción: la fotoprotección, barrera o medida para evitar problemas en la piel, se relaciona con el uso de protectores solares o prendas protectoras. Objetivos: la investigación determinó los conocimientos sobre fotoprotección en taxistas de la ciudad de Riobamba, Ecuador. Metodología: a partir de un estudio bibliográfico, descriptivo y analítico de campo se aplicó una encuesta mediante Microsoft Forms para Office 365, adaptada de Ramos et al. (2016), a 398 taxistas –hombres y mujeres– pertenecientes a diversas cooperativas, quienes aceptaron de manera voluntaria en un 94 % la socialización de resultados. Resultados: se identificó un 55 % en edades comprendidas entre 20 a 39 años, un predomino del género masculino (85 %); un 83 % afirmó poseer conocimientos sobre fotoprotección. Sin embargo, al evaluar hábitos de protección, solo el 47 % manifestó usar protector solar, el 65 % solo en días soleados, el 61 % se coloca antes de exponerse al sol y el 40 % para evitar quemaduras solares; el 86 % consideró necesaria una capacitación sobre temas de fotoprotección para evitar el fotoenvejecimiento. Área de estudio general: Salud. Área de estudio específica: Enfermería. Tipo de estudio: Bibliográfico
Trastornos electrolíticos inducidos por diuréticos de asa en los adultos mayores. Revisión sistemática
Authors: Guevara Sarmiento Judith , Martínez León Maritza del Rosario
Published: February 2024

Introducción: el uso crónico de medicamentos diuréticos de asa, desencadenan trastornos electrolíticos como parte de sus reacciones adversas (RAMS). Se ha observado que los adultos mayores tienden a desarrollar este tipo de RAMS, debido a los cambios en la función renal al envejecer. Objetivo: analizar los trastornos electrolíticos que provocan los diuréticos de asa en la población adulta mayor, según la evidencia científica publicada durante el periodo 2018-2023. Metodología: revisión sistemática documental, usando el método Prisma; se recopiló información de diferentes estudios retrospectivos, observacionales y transversales. Resultados: se observó una prevalencia significativa de hiponatremia, hipomagnesemia e hipocloremia por el uso crónico de diuréticos de asa, la edad de los pacientes fue de 60 años en adelante, con respecto al potasio, no queda claro si la hipo o hiperpotasemia es más frecuente, ya que los estudios sugieren una prevalencia similar de ambas condiciones. Conclusiones: por otro lado, los electrólitos menos alterados fueron el calcio y el fósforo.  La buena alimentación durante el uso de diuréticos de asa contribuye al equilibrio de varios electrólitos. Área de estudio general: Bioquímica y Farmacia. Área de estudio: Farmacología Clínica. Tipo de estudio: Artículo de revisión sistemática.
Water Dispersible Colloidal Metal and Metal Oxide Nanoparticles Synthesis for Agarwood Inoculation: A Review
Authors: Md Jahidul Islam  , Md Kawsar Uddin  , Mohammad Jakir Hossain
Published: February 2024

Agarwood is one of the most expensive natural products, produced from Aquilaria Spp. These plants produce agar resin through mechanical, biological, or chemical stress. Besides these, the particles having size in the range of 1-100 nm, are known as nanoparticles, have ultra-high surface area and surface plasmon resonance, can create stress on living plants when incorporated into the plant body. Adopting this concept, we intend to produce agarwood by inoculating water-dispersible metal/metal oxide nanoparticles. Therefore, we aim to synthesize water-dispersible four noble metals (Au, Ag, Pt, Pd) and five metal oxides (Fe3O4, TiO2, SnO2, MnO2, CuO) nanoparticles in aqueous colloidal states for application in biotechnology, considering the facile synthetic routes, readily available and inexpensive precursors, and stability of the products under normal laboratory conditions for an extended period, utilizing the synthesis methods of precipitation of metal/metal oxide nanoparticles from an aqueous solution, microwave and ultrasound-assisted rapid heating methods, sol-gel methods, and surfactant-assisted microemulsion methods. We believe that by applying any of these methods, water-dispersible, long-lasting colloidal metal/metal oxide nanoparticles can be synthesized using a simple laboratory setup, without the need for sophisticated tools or instruments.
IUBAT Review, 6(2): 41-76
Cost per NNT for upadacitinib in the treatment of patients with moderate-severe atopic dermatitis in Italy
Authors: Chiricozzi Andrea , Costanzo Antonio , Levi Anna , Parretta Federica , Ravasio Roberto
Published: February 2024

Background: Targeted systemic therapies, including abrocitinib, baricitinib, dupilumab, tralokinumab and upadacitinib, are new treatments for moderate to severe atopic dermatitis (AD). We evaluated the efficacy and the costs of these targeted systemic therapies in the treatment of adult patients with moderate to severe AD.
Methods: The clinical efficacy was assessed considering the results of a previous network meta-analysis (NMA). The analysis involved five therapies approved in Italy for the treatment of moderate to severe AD: abrocitinib (ABR), baricitinib (BAR), dupilumab (DUP), tralokinumab (TRA) and upadacitinib (UPA). According to the NMA, the cost of the treatment was based on the number of administrations dispensed at 16 weeks and the clinical efficacy was measured by the number needed to treat (NNT) compared to placebo using the improvement ≥ 75% (EASI-75) or ≥ 90 (EASI-90) from baseline of the eczema area and severity index (EASI). Only the ex-factory price of the targeted systemic therapies was considered. The cost per NNT was adopted as a cost-effectiveness indicator.
Results: At 16 weeks, the cost per NNT based on EASI-75 was lower for UPA 15 mg (€ 6,384.00) compared to BAR 4 mg (€ 11,619.73) and 2 mg (€ 14,524.66), ABR 100 mg (€ 16,265.22), DUP 300 mg (€ 16,115.04) and TRA 300 mg (€ 31,710.24). UPA 15 (€ 8,512.00) also showed the lower cost per NNT based on EASI-90 at 16 weeks compared to BAR 4 mg (€ 14,788.75) and 2 mg (€ 20,862.70), ABR 100 mg (€ 25,922.69), DUP 300 mg (€ 25,992.00) and TRA 300 mg (€ 41,067.36).
Conclusions: The findings show that upadacitinib is the most cost-effective option (cost per NNT) for the treatment of moderate to severe atopic dermatitis.
Implementasi Tradisi Pe’eppuk sebagai Bentuk Modal Sosial Masyarakat Desa Lohayong Flores Timur
Authors: Lestari Sri Putri Indah , Rahadian Septa , Badar Ali
Published: February 2024

Penelitian ini dilaksanakan di Desa Lohayong. Penelitian ini bertujuan untuk mengetahui wujud Modal sosial masyarakat Desa Lohayong. Penelitian ini menggunakan metode penelitian kualitatif dengan pendekatan Deskriptif kualitatif. Metode pengumpulan data dalam penelitian ini melalui observasi, wawancara, dan dokumentasi. Teknik analisa data dalam penelitian ini melalui teknik pengumpulan data, reduksi data, penyajian data, dan penarikan kesimpulan. Uji keabsahan data dalam penelitian ini menggunakan perpanjangan waktu, peningkatan ketekunan, dan Triangulasi. Penelitian ini menggunakan teori Modal Sosial milik Robert Putnam. Hasil penelitian ini menunjukan bahwa tradisi pe’eppuk (berkumpul) ini merupakan hajatan rutin yang diadakan oleh masyarakat desa Lohayong pertama kali sejak tahun 2000, dimana pada momen ini masyarakat desa Lohayong mengumpulkan ana uhur lewotana yang merantau di seluruh Indonesia dan Malaysia dan berlatar belakang simpati masyarakat terhadap kemajuan desa Lohayong dalam berbagai aspek. Selain aspek kemajuan desa, tradisi ini diadakan untuk memperat silaturahmi antar masyarakat desa Lohayong baik yang ada di desa maupun yang merantau diseluruh Indonesia dan Malaysia. Modal Sosial merupakan keberadaan seperangkat nilai yang mengendap tiga komponen utama yakni kepercayaan, norma, dan jejaring sosial untuk mencapai tujuan bersama. Ketika berbicara tentang modal sosial maka masyarakat desa lohayong memiliki modal sosial dalam mewujudkan tujuan bersama yang di jadikan niat utama diimplementasikan tradisi pe’eppuk ini. Sehingga dapat dikatakan bahwa tradisi pe’eppuk ini merupakan wujud atau bentuk modal sosial masyarakat Desa Lohayong Flores Timur
Isolation by disturbance: a pattern of genetic structure of the coral Pocillopora grandis in the Gulf of California
Authors: Dennis LE , Favoretto F , Balart EF , Munguía-Vega A , Sánchez-Ortiz C , Paz-García DA
Published: February 2024

Genetic differentiation within species can be influenced by the distance between populations (isolation by distance, IBD) or by differences in the habitats they occupy (isolation by environment, IBE). Understanding the population structure and dynamics that influence genetic diversity within small (<100 km2) multi-use marine protected areas (MPAs) and varying disturbances or protections is relevant to better designing and managing them. Pocillopora grandis communities were studied at 10 different sites in the Gulf of California, Mexico. The comparison between sites inside and outside an MPA or whether the population structure followed IBD or IBE was investigated with the genetic marker HSP70B. Environmental heterogeneity was used to test for IBE, and was measured by comparing the present benthic communities by site and through analysis of satellite images, while distance was used to test for IBD. Mantel tests found that the population of P. grandis did not follow IBD or IBE. Instead, cluster analysis grouped low (no-take zones) and high (crown-of-thorns starfish outbreaks and highly visited by tourists) disturbance sites together with the remaining sites within and outside of the MPA in another cluster (ΦRT = 0.082, p = 0.007). Furthermore, lower genetic diversity was found at sites with low and high disturbance. Our results suggest that instead of IBE or IBD influencing the population structure, the protection and disturbance levels could have a correlation with genetic patterns (i.e. isolation by disturbance, ID). These findings will be valuable for MPAs in terms of efficient management of genetic diversity objectives by considering the appropriate spacing and protections, depending on how vulnerable a species is to disturbance.
Aftereffects of Hurricanes Irma and Ian on queen conch Aliger gigas in the Florida Keys, USA
Authors: Voss JN , Sandbank E , Glazer RA , Delgado GA
Published: February 2024

Hurricanes can have substantial impacts on shallow-water marine habitats and species. Populations of slow-moving benthic species that are subject to depensation such as the queen conch Aliger gigas may be particularly vulnerable to hurricanes. The species is protected in Florida (USA), which allowed us to investigate the effects of Hurricanes Irma (2017) and Ian (2022) on the population without the confounding effects of fishing. Adult queen conch density had declined over 80% at sites resurveyed immediately after the passage of Hurricane Irma. Additionally, the sites closest to the eye of Hurricane Irma had a significant increase in the percent cover of sand. The sand mobilized by the storm likely buried numerous conch and caused mortality. Subsequent Florida Keys-wide annual monitoring did not show any substantial recovery in adult density prior to Hurricane Ian. Adult density had declined by ~45% at sites resurveyed after Hurricane Ian. Unlike after Irma, we did not detect any significant correlation in the change to the percent cover of sand with distance from Ian. This was probably because the eye of Hurricane Ian was farther away from the main portion of the Keys than that of Hurricane Irma. Nevertheless, after both hurricanes, adult density dropped below the minimum threshold for mating in the Keys, demonstrating the depensatory implications of hurricanes for conch populations. Consequently, fishery managers must consider the synergistic effects of hurricanes and harvest on exploited populations, especially since the intensity, longevity, and frequency of hurricanes are expected to increase due to climate change.
Broadening the antiviral landscape for influenza A virus
Authors:   , Pascha Mirte Nelleke
Published: February 2024

The potential of influenza A virus (IAV) to evolve and cross species barriers keeps it at the forefront of public health concerns. The endemic seasonal viruses are associated with a consistently high disease burden, and zoonotic infections pose a continuous threat of sparking the next pandemic. Consequently, there is an urgent need for broadly protective solutions for the control of IAV, including both vaccines and antivirals. A detailed characterization of antigenic sites on the viral glycoproteins, informed by the study of broadly neutralizing antibodies, contributes to this understanding and is vital to inform vaccine design. This thesis explores the development of a nanoparticle-based vaccine candidate and the discovery of monoclonal antibodies targeting the less examined neuraminidase glycoprotein. Additionally, it introduces a novel approach for the targeting of a recognized site for broadly neutralizing antibodies in the stem domain of the other glycoprotein hemagglutinin using macrocyclic peptides, an emerging class of antiviral agents. This research offers additional insights and innovative strategies that could contribute to the development of more effective interventions against IAV.
Heatalyzer: A Tool for Evaluating Indoor Comfort in Buildings during Extreme Heat Events
Authors: Capol Livia , Keshav Srinivasan , Nagy Zoltan
Published: February 2024

The increase in heatwaves due to climate change poses significant challenges to both indoor thermal comfort and occupant well-being. Unfortunately, existing work does not quantify the impact of extreme heat events as a function of building type, occupant age, and heatwave intensity and duration. We therefore present Heatalyzer, a novel Building Energy Modeling (BEM) tool to analyze indoor thermal comfort, liveability, and survivability for a range of buildings under both past and future weather scenarios. It lets users compare these outcomes across building types and weather scenarios, integrates algorithms for creating extreme weather data, and has a user-friendly interface. Additionally, it outputs several commonly-used thermal comfort metrics, which is crucial for evaluating indoor conditions during heatwaves. Using a case study, we show the potential of the tool to enable building managers and policy makers to assess the impact of extreme events on building occupants.
Cabra marcado para morrer
Authors: Batista Miguel Haoni
Published: February 2024

O texto procura, através das ferramentas da análise fí­lmica e do ensaio crí­tico, depreender os princí­pios de uma noção particular de cinema a partir do filme Cabra marcado para morrer (1984) de Eduardo Coutinho. O reencontro com Coutinho representou ao mesmo tempo um retorno à casa onde cresci e o ponto de chegada na maturação do olhar. Com seus filmes redescobri o cinema como ferramenta de investigação do mundo e o cineasta como funcionário do real, ou seja, alguém cujo trabalho é necessariamente condicionado por aquilo que a vida cotidiana lhe impõe. Outra experiência fundamental foi a imersão no ensaí­smo crí­tico. A prosa aqui é contaminada pela leitura apaixonada de crí­tica de cinema. Como lá, este texto só faz sentido para o leitor que viu o filme; ele é a primeira e mais importante referência. Da crí­tica herdamos, também, algumas ferramentas de abordagem e certa liberdade na escrita - às vezes garantida e estimulada pela universidade, às vezes não – e um investimento de fé na liberdade de leitura.
O Rastro: diálogos entre som e narrativa no gênero de horror.
Authors: Macagi Carlos Eduardo , Ito Bruno Almeida
Published: February 2024

Este artigo tem como objetivo realizar considerações sobre o processo produtivo experimental do média-metragem de gênero de horror intitulado "O Rastro" , realizado como TCC do curso de Cinema e Ví­deo da UNESPAR. O processo foi idealizado, a fim de atender a necessidade de uma participação criativa mais eficaz do processo de edição de som na produção como um todo, uma vez que esse é usualmente relegado a segundo plano em produções comerciais. A intenção foi criar um processo em que trilha, ambiência e sons dialogassem abertamente e influenciassem todos os processos criativos de produção, desde o roteiro até a montagem, além de, elementos do próprio gênero, para isso havendo necessidade de pensar novos mecanismos e dinâmicas de produção. Esse processo foi criativamente muito enriquecedor, proporcionando uma apropriação orgânica do som a narrativa, porém foi trabalhoso e demorado, indicando tal emprego em roteiros curtos e de equipe pequena e abrindo-se a novas proposições.
ViajoSola: Análise e autocrí­tica de uma primeira versão
Authors: Dias Esperança Hanna Henck
Published: February 2024

Este trabalho integra o primeiro capí­tulo do memorial artí­stico produzido para o trabalho de conclusão do curso de Cinema e Audiovisual da Universidade Estadual do Paraná (UNESPAR) - Campus Curitiba II, como espaço de reflexão dos processos do documentário de curta-metragem ViajoSola. Feito inteiramente com imagens de arquivo de viagens e com um texto de narração em voz over escrito em primeira pessoa e baseado nos relatos coletados em entrevistas, o documentário aborda a experiência de ser mulher e viajar sozinha, com o objetivo de criticar e denunciar a cultura machista que não consegue aceitar quando essas mulheres decidem viajar solo, que amedronta através da mí­dia e da violência. O documentário pretende ser, também, um incentivo para que mais mulheres ocupem espaços, seja por uma busca delas mesmas ou pela importância de se viajar sozinha como uma forma de protesto, uma parte da luta feminista e uma resistência ao machismo que as cercam. Neste estágio do memorial, será analisada uma primeira versão já existente do ViajoSola, os processos e as escolhas para que, a partir de uma auto-crí­tica, surja uma nova e última versão.
LIRION: RELATO DE UMA CAPTAÇÃO DE SOM DIRETO
Authors: Opolski Débora , Czmola Halyne , Meiring Rayssa
Published: February 2024

Este artigo é um relato reflexivo sobre o processo de captação de somdireto do filme curta-metragem Lirion, filmado no mês de maio de 2017. O som diretoconsiste sobretudo na gravação de elementos sonoros do filme no set de filmagem. Porconsequência, a qualidade sonora final do filme possui relação direta com a qualidade domaterial sonoro captado durante a gravação. Este relato pretende consolidar o trabalhoprático, através da reflexão, identificando procedimentos e processos que possam evitarsituações indesejadas durante a captação e evitar problemas posteriores na pós-produção.A captação foi realizada pelo Laboratório de Produção Sonora da Universidade Federaldo Paraná (UFPR), um laboratório que foi criado com o intuito de realizar pesquisas sobreprodução sonora vinculadas à prática profissional, inserindo os alunos no mundo do trabalho.Nesse projeto, a coordenação foi realizada pela prof. Dra. Débora Opolski e a captação desom foi realizada pelas alunas Halyne Czmola e Rayssa Meiring, integrantes do projeto.PALAVRAS-CHAVE: Laboratório de produção sonora UFPR. Cinema brasileiro. Relato deexperiência. Som direto. Captação.
A REPRESENTAÇÃO DAS PERSONAGENS MULHERES NA SÉRIE DINAMARQUESA FORBRYDELSEN
Authors: Ribeiro Gabriela Quadros
Published: February 2024

Este trabalho, parte da monografia de conclusão do curso de Cinema e Audiovisualda Unespar, tem como objetivo realizar uma análise da construção e representação daspersonagens femininas na produção seriada dinamarquesa Forbrydelsen, que é uma dasprecursoras do fenômeno atual das protagonistas detetives mulheres na TV. Partindo deteorias feministas relacionadas ao condicionamento do olhar em relação às personagensfemininas, o artigo busca indicar como se dá essa representação, tanto narrativamentequanto em relação a escolhas de linguagem audiovisual. A partir de episódios selecionadosdentre os 40 que compõem as quatro temporadas da série, busca-se ainda estabelecer quelugar essas mulheres ocupam dentro da narrativa em relação aos personagens masculinos,passando pelos conceitos de passividade e atividade, bem como questões relacionadas í sexualidade, famí­lia e trabalho. Por fim, em se tratando de linguagem, busca-se entenderqual o olhar que se tem sobre essa mulher, tanto dentro da série quanto fora dela, no quese refere ao olhar que se determina que o espectador tenha, tendo em vista ainda o opostoe questionando se essas mulheres também detêm o poder do olhar.PALAVRAS-CHAVE: Representação feminina. Forbrydelsen. Série televisiva criminal.
A DANÇA DE CADA UM'S
Authors: Borsatto Mabile
Published: February 2024

Ao procurar pela mobilidade e flexibilidade de distintos modos de fazer esolucionar do corpo nos processos de ensino e aprendizagem encontramos o UM–Núcleode Pesquisa Artí­stica da UNESPAR/FAP, onde na promoção do entrelaçamento entreperspectivas de diferentes sujeitos e contextos, realiza-se uma dança como um espaçode percepções hí­bridas capaz de inventar, transformar, aprimorar e ampliar sentidos.Este artigo discute, então, que os modos onde há hierarquia centralizadora de poder,mecanismos de dominação e estruturas sólidas e pouco flexí­veis nas relações de ensinoe aprendizagem, sobrepondo a experiência, podem ser subvertidos pelo diálogo horizontalentre o conhecimento especializado e o conhecimento do senso comum. Nesse sentido,percebe-se que as experiências existentes no UM não se reduzem, nem se simplificam,mas geram uma nova tessitura entre o conhecido e o desconhecido, entre o convencionale o inovador, entre a ordem e a desordem. A possibilidade de situar a diferença comocondições de existência da dança dentro do UM, atua como um sinal de reconhecimentopara a construção de lógicas de conduta e operacionalidades em dança que incidem comoprincí­pios norteadores da auto-observação e do autoconhecimento e permite que outrosmodos de ensinar e aprender sejam inventados. Desse modo, o artigo instaura-se comoum convite e se efetiva em um encontro com a dança, e não um encontro qualquer, masdaqueles que incitam a fazer diferente através das relações e é também uma crí­tica, masnão aquela convencional de alguém que assiste de longe, mas da possibilidade de umacrí­tica colaborativa, para que todos os interessados em educação possam dialogar e criarsuas reflexões.PALAVRAS-CHAVE: UM's. Conhecimento especializado. Senso comum. Ensino eAprendizagem.
O ELO E A CONECTIVIDADE: TEATRO E BLACK MIRROR EM DISCUSSÃO
Authors: Pinheiro Freitas Ana Carla
Published: February 2024

Os termos elo e conectividade podem ser usados como sinônimos em alguns contextos ou situações. Assim, é possí­vel dizer que " o cinema e o teatro podem formar um elo entre um tipo de expressão artí­stica e o outro" ou que a "conectividade com a arte pode se dar por meio do cinema e/ou do teatro" . No presente texto, os termos "elo" e "conectividade" são alocados de acordo com o objeto ao qual se referem mais especificamente. O elo que une o discurso e os temas no contexto da peça teatral "o escândalo Phillipe Dussaert" são distintos da conectividade relacionada ao episódio da série black mirror intitulado smithreens. O cerne da discussão diz respeito ao papel, à transformação e à atualização de formas de expressão artí­stica, tais como aquelas que foram concebidas na peça teatral e no episódio da polêmica série.
Numerical Investigations of Outer-Layer Turbulent Boundary Layer Control for Drag Reduction Through Micro Fluidic-Jet Actuators
Authors: Li Li , Wu Junsheng , Liang Yihua , Tian Zengdong
Published: February 2024

Drag reduction through turbulent boundary layer control (TBLC) is an essential way to develop green aviation technologies. Compared with traditional approaches for drag reduction, turbulence drag reduction is a relatively new technology, particularly for skin friction drag reduction, and it is becoming a hotspot problem worldwide. This paper focuses on the research of micro fluidic-jet actuators used for outer-layer boundary layer control with high-performance computing (HPC). This study aims to reduce turbulent drag by reshaping the flow structure within the turbulent boundary layer. To ensure the calculation accuracy of the core region and reduce the consumption of computing resources, a zonal LES/RANS strategy and WMLES method are proposed to simulate the effects of fluidic-actuators for outer-layer boundary control, in which high-performance computing has to be involved. The studies are performed on the classical zero-gradient turbulent flat plate cases, in which three different control strategies named “W-control,” “V-control,” and “VW-control” are used and compared to study the effects of drag reduction under a low Reynolds number at Reτ = 470 and a higher Reynolds number at Reτ = 4700. The mechanism for drag reduction is analysed via a pre-multiplied spectral method and a parallel dynamic mode decomposition (DMD) method. The results show that the present approach can effectively simulate the outer-layer turbulent boundary control where the “V-control” with the fluidic-jet actuator array behaves well to achieve an average drag reduction (DR) rate of more than 5% for the high Reynolds number case of the flat plate boundary layer. The high Reynolds shear stress and turbulent kinetic energy distribution in the boundary layer region show an obvious uplift under the effects of actuators, which is the main mechanism for drag reduction.
A versatile kinase mobility shift assay (KiMSA) for PKA analysis and cyclic AMP detection in sperm physiology (and beyond)
Authors: Novero Analia G. , Curcio Catalina , Steeman Tomás J. , Binolfi Andres , Krapf Diego , Buffone Mariano G. , Krapf Dario , Stival Cintia
Published: February 2024

The cAMP-dependent protein kinase (PKA) is one of the most extensively distributed kinases among intracellular signal cascades, with a pivotal role in the regulation of various processes, including the capacitation of sperm cells. Traditional assessments of PKA activity relies on the utilization of [γ-32P] ATP and the Kemptide substrate. This methodology presents several major drawbacks, including high-costs and health risks derived from the manipulation of radioactive isotopes. In this work we introduce an enhanced non-radioactive assay for quantifying PKA activity, termed KiMSA which relies on the use of a fluorescent-labeled Kemptide (Kemptide-FITC). Once the kinase reaction is terminated, the products can be easily resolved through electrophoresis on an agarose gel and quantified by fluorescence densitometry. We show that the KiMSA assay is suitable for purified PKA, and also to address both basal and capacitation induced PKA activity in mouse sperm cells. Furthermore, the assay enables monitoring the inhibition of PKA with inhibitors such as sPKI and H-89 in live cells. Therefore, the experimental and optimal assay conditions are set so that the KiMSA assay can be used to either assess in vitro as well as in vivo PKA activity in sperm cells. Finally, this method allows for measurement of cAMP concentrations, rendering a versatile technique for the study of cAMP/PKA pathways.
Maternal vgll4a regulates zebrafish epiboly through Yap1 activity
Authors: Camacho-Macorra Carlos , Tabanera Noemí , Sánchez-Bustamante Elena , Bovolenta Paola , Cardozo Marcos J.
Published: February 2024

Gastrulation in zebrafish embryos commences with the morphogenetic rearrangement of blastodermal cells, which undergo a coordinated spreading from the animal pole to wrap around the egg at the vegetal pole. This rearrangement, known as epiboly, relies on the orchestrated activity of maternal transcripts present in the egg, compensating for the gradual activation of the zygotic genome. Epiboly involves the mechano-transducer activity of yap1 but what are the regulators of yap1 activity and whether these are maternally or zygotically derived remain elusive. Our study reveals the crucial role of maternal vgll4a, a proposed Yap1 competitor, during zebrafish epiboly. In embryos lacking maternal/zygotic vgll4a (MZvgll4a), the progression of epiboly and blastopore closure is delayed. This delay is associated with the ruffled appearance of the sliding epithelial cells, decreased expression of yap1-downstream targets and transient impairment of the actomyosin ring at the syncytial layer. Our study also shows that, rather than competing with yap1, vgll4a modulates the levels of the E-cadherin/β-catenin adhesion complex at the blastomeres’ plasma membrane and hence their actin cortex distribution. Taking these results together, we propose that maternal vgll4a acts at epiboly initiation upstream of yap1 and the E-cadherin/β-catenin adhesion complex, contributing to a proper balance between tissue tension/cohesion and contractility, thereby promoting a timely epiboly progression.
Interplay between mitochondrial dysfunction and lysosomal storage: challenges in genetic metabolic muscle diseases with a focus on infantile onset Pompe disease
Authors: Zhang Mengjiao , Niu Jiechao , Xu Mengmeng , Wei Erhu , Liu Peng , Sheng Guangyao
Published: February 2024

Background

Pompe disease (PD) is a rare, progressive autosomal recessive lysosomal storage disorder that directly impacts mitochondrial function, leading to structural abnormalities and potentially culminating in heart failure or cardiogenic shock. The clinical course and molecular mechanisms of the disease remain incompletely understood.

Methods

We performed a retrospective analysis to examine the clinical manifestations, genetic traits, and the relationship between PD and mitochondrial function in a pediatric patient. This comprehensive evaluation included the use of ultrasound echocardiograms, computed tomography (CT) scans, electrocardiograms, mutagenesis analysis, and structural analysis to gain insights into the patient's condition and the underlying mechanisms of PD. For structural analysis and visualization, the structure of protein data bank ID 5KZX of human GAA was used, and VMD software was used for visualization and analysis.

Results

The study revealed that a 5-month-old male infant was admitted due to fever, with physical examination finding abnormal cardiopulmonary function and hepatomegaly. Laboratory tests and echocardiography confirmed heart failure and hypertrophic cardiomyopathy. Despite a week of treatment, which normalized body temperature and reduced pulmonary inflammation, cardiac abnormalities did not show significant improvement. Further genetic testing identified a homozygous mutation c.2662G>T (p.E888) in the GAA gene, leading to a diagnosis of Infantile-Onset Pompe Disease (IOPD).

Conclusions

Although enzyme replacement therapy can significantly improve the quality of life for patients with PD, enhancing mitochondrial function may represent a new therapeutic strategy for treating PD.
Whole-exome sequencing in familial type 2 diabetes identifies an atypical missense variant in the RyR2 gene
Authors: Bansal Vikas , Winkelmann Bernhard R. , Dietrich Johannes W. , Boehm Bernhard O.
Published: February 2024

Genome-wide association studies have identified several hundred loci associated with type 2 diabetes mellitus (T2DM). Additionally, pathogenic variants in several genes are known to cause monogenic diabetes that overlaps clinically with T2DM. Whole-exome sequencing of related individuals with T2DM is a powerful approach to identify novel high-penetrance disease variants in coding regions of the genome. We performed whole-exome sequencing on four related individuals with T2DM – including one individual diagnosed at the age of 33 years. The individuals were negative for mutations in monogenic diabetes genes, had a strong family history of T2DM, and presented with several characteristics of metabolic syndrome. A missense variant (p.N2291D) in the type 2 ryanodine receptor (RyR2) gene was one of eight rare coding variants shared by all individuals. The variant was absent in large population databases and affects a highly conserved amino acid located in a mutational hotspot for pathogenic variants in Catecholaminergic polymorphic ventricular tachycardia (CPVT). Electrocardiogram data did not reveal any cardiac abnormalities except a lower-than-normal resting heart rate (< 60 bpm) in two individuals – a phenotype observed in CPVT individuals with RyR2 mutations. RyR2-mediated Ca2+ release contributes to glucose-mediated insulin secretion and pathogenic RyR2 mutations cause glucose intolerance in humans and mice. Analysis of glucose tolerance testing data revealed that missense mutations in a CPVT mutation hotspot region – overlapping the p.N2291D variant – are associated with complete penetrance for glucose intolerance. In conclusion, we have identified an atypical missense variant in the RyR2 gene that co-segregates with diabetes in the absence of overt CPVT.
Prevalence and clinical correlates of hyperhomocysteinemia in Chinese urban population with hypertension
Authors: Xu Yayun , Feng Haixing , Zhang Liping , Li Yanlei , Chi Feng , Ren Lijie
Published: February 2024

Context

The coexistence of hypertension and elevated homocysteine (Hcy) levels has a mutually reinforcing impact on the susceptibility to cardio-cerebrovascular disease.

Objective

The aim was to assess the prevalence, clinical correlation, and demographic characteristics of hyperhomocysteinemia (HHcy) within the Chinese urban population with hypertension.

Methods

A cohort of 473 individuals with hypertension were selected from four communities in Shenzhen, China. Demographic attributes, clinical profiles, and lifestyle behaviors were gathered and compared between individuals with and without HHcy. A logistic regression model was employed to examine potential factors associated with the prevalence of HHcy. Correlation between Hcy levels and clinical characteristics was assessed through multiple linear regression analysis.

Results

The prevalence of HHcy in the population with hypertension was 31.3%. In comparison to individuals without HHcy, those with HHcy exhibited a higher proportion of males, a higher prevalence of smoking and alcohol consumption, and a higher proportion of cases with the homozygous (TT) genotype at the MTHFR C677T polymorphism. Moreover, individuals with HHcy had lower levels of folic acid (FA), and lower fruit and vitamin B12 intake. Furthermore, the risk factors for HHcy were male (B = 1.430, OR = 4.179) and MTHFR (TT) (B = 1.086, OR = 2.961). In addition, the multiple linear regression analysis revealed a significant association between Hcy levels and gender (B = -2.784, P = 0.004), MTHFR genotypes (B = 1.410, P = 0.005), and FA levels (B = -0.136, P = 0.030).

Conclusion

The high prevalence of HHcy among hypertensive patients in this Chinese urban population underscores the necessity for interventions targeting modifiable risk factors such as dietary choices and lifestyle practices.
China contributed to low-carbon development: carbon emission increased but carbon intensity decreased
Authors: Huang Chunbo , Gan Xintao , Wan Yunshan , Jin Lin , Teng Jiaxi , Li Zeyu
Published: February 2024

Introduction

Population expansion and economic development increased global greenhouse gas emissions, leading to serious environmental degradation. China, the world's largest developing country and promoter of the “Belt and Road Initiative” (BRI), accounts for 28.8% of the world"s total energy carbon emissions. How to reduce energy consumption to achieve the “double carbon” target (i.e., carbon peaking and carbon neutrality) and promote the implementation of Green BRI is still a serious challenge that China needs to face.

Methods

We evaluated China's carbon emissions using three indicators (i.e., total carbon emission, carbon intensity, and carbon emissions effect), and used spatial analysis to reveal the spatial and temporal trends of China's carbon emissions. In addition, the LMDI model was adopted to explore the driving mechanism of carbon emissions, so as to seek a path that can achieve harmonious economic and environmental development, as well as the “double carbon” target.

Results

China's total carbon emission increased at a rate of 226.12% from 2000 to 2019, while the carbon intensity decreased at a rate of 48.84%. Carbon emission showed a trend of increasing and then decreasing from southwest to northeast. From 2000 to 2019, the total carbon emission, Gross Domestic Product (GDP), population size and total energy consumption are growing in synergy. Economic and population effects are positively related to carbon emissions, while technology effects are negatively related to it, indicating technological innovations contribute to the reduction of carbon emissions.

Discussion

Some suggestions were proposed to control carbon emissions with a view to helping policy makers to formulate relevant policies. The findings provide a scientific basis and reference for the country to achieve the “double carbon” target and the low-carbon sustainable development of BRI.
Characterisation of the in-vivo miRNA landscape in Drosophila ribonuclease mutants reveals Pacman-mediated regulation of the highly conserved let-7 cluster during apoptotic processes
Authors: Bernard Elisa I. M. , Towler Benjamin P. , Rogoyski Oliver M. , Newbury Sarah F.
Published: February 2024

The control of gene expression is a fundamental process essential for correct development and to maintain homeostasis. Many post-transcriptional mechanisms exist to maintain the correct levels of each RNA transcript within the cell. Controlled and targeted cytoplasmic RNA degradation is one such mechanism with the 5′-3′ exoribonuclease Pacman (XRN1) and the 3′-5′ exoribonuclease Dis3L2 playing crucial roles. Loss of function mutations in either Pacman or Dis3L2 have been demonstrated to result in distinct phenotypes, and both have been implicated in human disease. One mechanism by which gene expression is controlled is through the function of miRNAs which have been shown to be crucial for the control of almost all cellular processes. Although the biogenesis and mechanisms of action of miRNAs have been comprehensively studied, the mechanisms regulating their own turnover are not well understood. Here we characterise the miRNA landscape in a natural developing tissue, the Drosophila melanogaster wing imaginal disc, and assess the importance of Pacman and Dis3L2 on the abundance of miRNAs. We reveal a complex landscape of miRNA expression and show that whilst a null mutation in dis3L2 has a minimal effect on the miRNA expression profile, loss of Pacman has a profound effect with a third of all detected miRNAs demonstrating Pacman sensitivity. We also reveal a role for Pacman in regulating the highly conserved let-7 cluster (containing miR-100, let-7 and miR-125) and present a genetic model outlining a positive feedback loop regulated by Pacman which enhances our understanding of the apoptotic phenotype observed in Pacman mutants.