bluuebunny/crossref_metadata_2025 huggingface dataset:
‘Sharing’ as a Critical Framework for Waterfront Heritage Regeneration: A Case Study of Suzhou Creek, Shanghai
Authors: Zhu Yichen , Li Zhenyu
Published: August 2024

The purpose of this study was to analyze ‘sharing’ as an operational framework for waterfront industrial heritage revitalization in the context of sustainable urban regeneration. This research study was conducted to better understand the public accessibility of heritage preservation projects in a densely populated waterfront urban area and to determine to what extent heritage could be made available to the general public. We examined the development of industrial heritage along Suzhou Creek, Shanghai, and its process of regeneration. The focus area covered a waterway stretch of 19.2 km and an adjacent land area of 11.7 km2 managed as a single planning entity on both sides of the creek. We analyzed the present preservation practices and discovered a growing desire to increase the historical buildings’ visibility in the context of urban regeneration. We argue that ‘sharing’ can serve as a pivotal framework for sustainable waterfront regeneration, as its implementation can (1) increase the public value of waterfront heritage and (2) incorporate comprehensive objectives, design strategies, evaluation methods, and public participation into the space revitalization process.
The Mclust Analysis of Tumor Budding Unveils the Role of the Collagen Family in Cervical Cancer Progression
Authors: Lee Olive EM , Le Tan Minh , Chong Gun Oh , Cho Junghwan Joshua , Park Nora Jee-Young
Published: August 2024

In RNA-seq data analysis, condensing the gene count matrix size is pivotal for downstream investigations, particularly pathway analysis. For this purpose, harnessing machine learning attracts increasing interest, while conventional methodologies depend on p-value comparisons. In this study, 20 tissue samples from real-world cervical cancers were subjected to sequencing, followed by the application of the Mclust algorithm to delineate an optimal cluster. By stratifying tumor budding into high and low groups and quantifying the epithelial-to-mesenchymal transition (EMT) score to scrutinize tumor budding, we discerned 24 EMT-related genes, with 5 showing strong associations with cervical cancer prognosis. Our observations elucidate a biological flow wherein EMT, Matrix Metallopep-tidase 2 (MMP2), and extracellular matrix (ECM) degradation are interconnected, ultimately leading to collagen type VI and exacerbating the prognosis of cervical cancer. The present study underscores an alternative method for selecting useful EMT-related genes by employing an appropriate clustering algorithm, thereby avoiding classical methods while unveiling novel insights into cervical cancer etiology and prognosis. Moreover, when comparing high and low tumor budding, collagen type VI emerges as a potential gene marker for the prognosis of cervical cancer.
The Co-Occurrence of 22q11.2 Deletion Syndrome and Epithelial Basement Membrane Dystrophy: A Case Report and Review of the Literature
Authors: Armentano Marta , Alisi Ludovico , Giovannetti Francesca , Iannucci Valeria , Lucchino Luca , Bruscolini Alice , Lambiase Alessandro
Published: August 2024

Background: 22q11.2 deletion syndrome (22q11.2DS) is a genetic disorder caused by the deletion of the q11.2 band of chromosome 22. It may affect various systems, including the cardiovascular, immunological, gastrointestinal, endocrine, and neurocognitive systems. Additionally, several ocular manifestations have been described. Results: We report a case of a 34-year-old female diagnosed with 22q11.2DS who presented with visual discomfort and foreign body sensation in both eyes. She had no history of recurrent ocular pain. A comprehensive ophthalmological examination was performed, including anterior segment optical coherence tomography and in vivo confocal microscopy. Overall, the exams revealed bilateral corneal map-like lines, dots, and fingerprint patterns, consistent with a diagnosis of epithelial basement membrane dystrophy (EBMD). In addition to presenting with this novel corneal manifestation for 22q11.2 DS, we review the ocular clinical features of 22q11.2DS in the context of our case. Conclusions: The EBMD may represent a new corneal manifestation associated with 22q11.2 syndrome, although the link between these conditions is unknown. Further research is warranted to investigate potentially shared genetic or molecular pathways to the understanding of the phenotypic variety observed among this rare syndrome.
Suitability Study of Optical Coordinate Measuring Machine for Quality Assessment and Wear Phenomena Identification of Blade Edge and Surface of Planer Technical Knives
Authors: Rzepka Magdalena , Łukianowicz Czesław , Zawadka Wojciech , Rokosz Krzysztof , Nadolny Krzysztof
Published: August 2024

This article discusses a comparative analysis of the wear and quality of planer knife blades used in wood planers. The novelty in this work is the use of a simple coordinate measuring machine with a vision system to assess the wear of the cutting edges of planer knives. The primary objective of the research described in this paper was to verify whether the wear of the cutting edge of planer knives can be measured quickly and accurately using an optical coordinate measuring machine with a vision system. To date, contact profilometry methods have been used for this purpose, which require a specialist apparatus and qualified measuring equipment operators and are expensive and time-consuming. The research presented in this work was conducted on twelve planer knives. The condition and wear of the working surfaces of the tested knives were assessed using an optical digital microscope. The wear of the cutting edge of the knives was measured using two methods: the contact profilometry method and an optical coordinate measuring machine equipped with a vision system. The edge profiles and their parameters obtained by the optical method were compared to the results of measurements with a stylus profilometer. Based on the research and analyses conducted, it was found that the optical method used in this research significantly shortens the time of measuring the wear of the cutting edges of planer knives. In addition, this method has a wider measurement range, and the obtained measurement results are characterized by lower measurement uncertainty.
Modified Epoxy Resin on the Burning Behavior and Mechanical Properties of Aramid Fiber Composite
Authors: Lan Xuke , Bian Chenxi , Yang Yunxian , Zhang Qi , Huang Guangyan
Published: August 2024

Aramid fiber/epoxy resin (AF/EP) composite has been heavily used as an impact protection material due to its excellent mechanical properties and lightweight merits. Meanwhile, it is also necessary to concern the flammability of matrix resin and the wick effect of aramid fiber, which would constitute a fire risk in harsh environments. In this work, a multifunctional flame-retardant modifier (EAD) was incorporated into the AF/EP system to improve the flame retardation. The addition of 5 wt% EAD made the AF/EP composite exhibit a high limiting oxygen index (LOI) value of 37.5%, self-extinguishment, as well as decreased total heat release and total smoke release. The results from thermogravimetric analysis (TGA) and dynamic mechanical analysis (DMA) demonstrated that the treated composites maintained good thermal stability. Due to the combined action of covalent and noncovalent bonds in the matrix-rich region, the interfacial bonding improved, which endowed AF/EP composite with strengthening and toughening effects. Compared with the control sample AF/EP, the tensile strength and ballistic parameter (V50) of the sample with 5 wt% EAD increased by 17% and 10%, accompanied with ductile failure mode. Furthermore, the flame-retardant mechanism was obtained by analyzing the actions in condensed and gaseous phases. Thanks to good compatibility and interfacial adhesion, the incorporation of EAD solved the inconsistent issue between flame retardancy and mechanical properties, which further expanded the application of AF/EP composite in the protection field.
Synthetic Time Series Generation for Decision Intelligence Using Large Language Models
Authors: Grigoraș Alexandru , Leon Florin
Published: August 2024

A model for generating synthetic time series data using pre-trained large language models is proposed. Starting with the Google T5-base model, which employs an encoder–decoder transformer architecture, the model underwent pre-training on diverse datasets. It was then fine-tuned using the QLoRA technique, which reduces computational complexity by quantizing weight parameters. The process involves the tokenization of time series data through mean scaling and quantization. The performance of the model was evaluated with fidelity, utility, and privacy metrics, showing improvements in fidelity and utility but a trade-off with reduced privacy. The proposed model offers a foundation for decision intelligence systems.
Person Re-Identification in Special Scenes Based on Deep Learning: A Comprehensive Survey
Authors: Chen Yanbing , Wang Ke , Ye Hairong , Tao Lingbing , Tie Zhixin
Published: August 2024

Person re-identification (ReID) refers to the task of retrieving target persons from image libraries captured by various distinct cameras. Over the years, person ReID has yielded favorable recognition outcomes under typical visible light conditions, yet there remains considerable scope for enhancement in challenging conditions. The challenges and research gaps include the following: multi-modal data fusion, semi-supervised and unsupervised learning, domain adaptation, ReID in 3D space, fast ReID, decentralized learning, and end-to-end systems. The main problems to be solved, which are the occlusion problem, viewpoint problem, illumination problem, background problem, resolution problem, openness problem, etc., remain challenges. For the first time, this paper uses person ReID in special scenarios as a basis for classification to categorize and analyze the related research in recent years. Starting from the perspectives of person ReID methods and research directions, we explore the current research status in special scenarios. In addition, this work conducts a detailed experimental comparison of person ReID methods employing deep learning, encompassing both system development and comparative methodologies. In addition, we offer a prospective analysis of forthcoming research approaches in person ReID and address unresolved concerns within the field.
Ultrasound Depolymerization and Characterization of Poly- and Oligosaccharides from the Red Alga Solieria chordalis (C. Agardh) J. Agardh 1842
Authors: Lesgourgues Mathilde , Latire Thomas , Terme Nolwenn , Douzenel Philippe , Leschiera Raphaël , Lebonvallet Nicolas , Bourgougnon Nathalie , Bedoux Gilles
Published: August 2024

Red seaweed carrageenans are frequently used in industry for its texturizing properties and have demonstrated antiviral activities that can be used in human medicine. However, their high viscosity, high molecular weight, and low skin penetration limit their use. Low-weight carrageenans have a reduced viscosity and molecular weight, enhancing their biological properties. In this study, ι-carrageenan from Solieria chordalis, extracted using hot water and dialyzed, was depolymerized using hydrogen peroxide and ultrasound. Ultrasonic depolymerization yielded fractions of average molecular weight (50 kDa) that were rich in sulfate groups (16% and 33%) compared to those from the hydrogen peroxide treatment (7 kDa, 6% and 9%). The potential bioactivity of the polysaccharides and low-molecular-weight (LMW) fractions were assessed using WST-1 and LDH assays for human fibroblast viability, proliferation, and cytotoxicity. The depolymerized fractions did not affect cell proliferation and were not cytotoxic. This research highlights the diversity in the biochemical composition and lack of cytotoxicity of Solieria chordalis polysaccharides and LMW fractions produced by a green (ultrasound) depolymerization method.
Lipids Extracted from Aptocyclus ventricosus Eggs Possess Immunoregulatory Effects on RAW264.7 Cells by Activating the MAPK and NF-κB Signaling Pathways
Authors: Lee Seul Gi , Rod-in Weerawan , Jung Jun Jae , Jung Seok Kyu , Lee Sang-min , Park Woo Jung
Published: August 2024

This study was conducted to evaluate the potential anti-inflammatory and immune-enhancement properties of lipids derived from Aptocyclus ventricosus eggs on RAW264.7 cells. Firstly, we determined the fatty acid compositions of A. ventricosus lipids by performing gas chromatography analysis. The results showed that A. ventricosus lipids contained saturated fatty acids (24.37%), monounsaturated fatty acids (20.90%), and polyunsaturated fatty acids (54.73%). They also contained notably high levels of DHA (25.91%) and EPA (22.05%) among the total fatty acids. Our results for the immune-associated biomarkers showed that A. ventricosus lipids had immune-enhancing effects on RAW264.7 cells. At the maximum dose of 300 µg/mL, A. ventricosus lipids generated NO (119.53%) and showed greater phagocytosis (63.69%) ability as compared with untreated cells. A. ventricosus lipids also upregulated the expression of iNOS, IL-1β, IL-6, and TNF-α genes and effectively upregulated the phosphorylation of MAPK (JNK, p38, and ERK) and NF-κB p65, indicating that these lipids could activate the MAPK and NF-κB pathways to stimulate macrophages in the immune system. Besides their immune-enhancing abilities, A. ventricosus lipids significantly inhibited LPS-induced RAW264.7 inflammatory responses via the NF-κB and MAPK pathways. The results indicated that these lipids significantly reduced LPS-induced NO production, showing a decrease from 86.95% to 38.89%. Additionally, these lipids downregulated the expression of genes associated with the immune response and strongly suppressed the CD86 molecule on the cell surface, which reduced from 39.25% to 33.80%. Collectively, these findings imply that lipids extracted from A. ventricosus eggs might have biological immunoregulatory effects. Thus, they might be considered promising immunomodulatory drugs and functional foods.
Interleukin-6 (-174G/C), Interleukin-1β (-511 C/T), and Apolipoprotein B-100 (2488 C/T) Gene Polymorphism in Pre-Eclampsia
Authors: Najeeb Muhammad Naveed , Munir Umaira , Hamza Muhammad Ameer , Mehmood Sadia , Qureshi Javed Anver , Maqbool Tahir
Published: August 2024

Background and objectives: Pre-eclampsia (PE) is a pregnancy-specific condition characterized by significant health risks for pregnant women worldwide due to its status as a multi-organ disorder. High blood pressure (hypertension) with or without proteinuria is usually considered an initial clinical sign of PE. The pathogenesis of pre-eclampsia is highly complex and likely involves multiple factors, including poorly developed uterine spiral arterioles, immunological issues, placental ischemia or infarction, and genetic abnormalities. Inflammatory cytokine production, regulated by cytokine gene polymorphisms, is one of the factors likely contributing to the development of PE. The present study aimed to assess IL-6, IL-1β, and Apo B-100 gene polymorphism and to evaluate the association of these polymorphisms with PE. Materials and Methods: This cross-sectional observational study involved 99 participants aged 16 to 45 years from Bahawal Victoria Hospital Bahawalpur, Punjab, Pakistan. The participants were divided into three groups: Group 1 (PE with severe hypertension), Group 2 (PE with hypertension), and Group 3 (control), each comprising 33 individuals. Maternal blood samples were collected, DNA was extracted, and molecular genetic analysis of the IL-6, IL-1β, and Apo B-100 genes was performed using the PCR-RFLP method. Allelic frequencies were compared, and statistical analysis was conducted using SPSS 25, applying the Hardy–Weinberg equation and chi-square test to evaluate the results. Results: There are differences in the distribution of allelic frequencies for IL-6 -174G/C (CC, GC, GG), IL-1β-511C/T (CC, CT, TT), and Apo B-100 2488 C/T (CC, CT, TT) between pre-eclamptic patients and the control group. The analysis using the Hardy–Weinberg equilibrium and chi-square test showed an association between the IL-6-174 G/C polymorphism and the severity of pre-eclampsia. Conclusions: The polymorphisms of the IL-6, IL-1β, and Apo B-100 genes revealed different alleles. The IL-6 gene alone was found to be in disequilibrium according to the Hardy–Weinberg equation, indicating a potential link to the severity of pre-eclampsia in the population studied.
Atrial Fibrillation Prevalence Rates and Its Association with Cardiovascular–Kidney–Metabolic Factors: SIMETAP-AF Study
Authors: Ruiz-García Antonio , Serrano-Cumplido Adalberto , Escobar-Cervantes Carlos , Arranz-Martínez Ezequiel , Pallarés-Carratalá Vicente
Published: August 2024

Background and Objectives: Atrial fibrillation (AF) is the most frequent arrhythmia and the main cause of hospital admissions for cardioembolic stroke. The SIMETAP research project aims to update the prevalence rates of cardiovascular, renal, or metabolic factors and to evaluate their respective associations with factors that could be related. The present study aims to assess the AF prevalence rates in an adult population and its association with cardiovascular–kidney–metabolic (CKM) factors. Materials and Methods: This cross-sectional observational study was conducted in a primary care setting, with a population-based random sample of 6588 people aged 18.0–102.8 years. Crude and adjusted prevalence rates of AF were calculated. The associations of CKM factors with AF were assessed using bivariate and multivariate analysis. Results: The age- and sex-adjusted prevalence rates of AF were 2.9% in the overall adult population, 6.1% in the population aged ≥50 years, and 12.9% in the population aged ≥70 years, with no significant differences by sex. AF prevalence in the population under 50 years of age barely reached 1‰. Heart failure (HF), hypertension, chronic kidney disease (CKD), stroke, low HDL-cholesterol, and prediabetes were independent CKM factors associated with AF in the overall population, as were the same factors, except prediabetes, in the population ≥50 years old (p < 0.001). High or very high vascular risk was present in 92.4% [95% CI: 89.1–95.7]) of the population with AF. Conclusions: The adjusted prevalence rate of AF in the population aged 50 years or older was 6.1%, twice that of the overall adult population and half that of the population aged 70 years or older. The main independent CKM factors associated with AF were HF, stroke, CKD, hypertension, and low HDL-cholesterol.
Laundry Isolate Delftia sp. UBM14 Capable of Biodegrading Industrially Relevant Aminophosphonates
Authors: Riedel Ramona , Meißner Karsten , Kaschubowski Arne , Benndorf Dirk , Martienssen Marion , Braun Burga
Published: August 2024

Phosphonates such as ethylenediaminetetra (methylenephosphonic acid) (EDTMP) and aminotris (methylenephosphonic acid) (ATMP) are used every day in water treatment processes or in household products. Their consumption is still increasing, regardless of the debates on their environmental impact. Here, the microbial characterisation and determination of the biodegradation potential of selected industrially relevant phosphonates for the isolate Delftia sp. UMB14 is reported. The opportunistic strain was isolated from a biofilm that was derived from a conventional washing machine using conventional detergents containing phosphonates. In antimicrobial susceptibility testing, the strain was only susceptible to sulfonamide, tetracycline, and chloramphenicol. Physiological and biochemical characteristics were determined using the BIOLOG EcoPlate assay. Most importantly, the strain was shown to convert D-malic acid and D-mannitol, as confirmed for strains of Delftia lacustris, and thus the new isolate could be closely related. Biodegradation tests with different phosphonates showed that the strain preferentially degrades ATMP and EDTMP but does not degrade glyphosate (GS) and amino (methylphosphonic acid) (AMPA). A specific gene amplification confirmed the presence of phnX (phosphonoacetaldehyde hydrolase) and the absence of PhnJ (the gene for the core component of C–P lyase). The presence of PhnCDE is strongly suggested for the strain, as it is common in Delftia lacustris species.
Short-Term Effects of Cenchrus fungigraminus/Potato or Broad Bean Interplanting on Rhizosphere Soil Fertility, Microbial Diversity, and Greenhouse Gas Sequestration in Southeast China
Authors: Li Jing , Lei Yufang , Wen Yeyan , Zhu Jieyi , Di Xiaoyue , Zeng Yi , Han Xiao , Que Zuhui , Mediatrice Hatungimana , Rensing Christopher , Lin Zhanxi , Lin Dongmei
Published: August 2024

Cenchrus fungigraminus is a new species and is largely used as forage and mushroom substrate. However, it can usually not be planted on farmland on account of local agricultural land policy. Interplanting Cenchrus fungigraminus with other crops annually (short-term) is an innovative strategy to promote the sustainable development of the grass industry in southern China. To further investigate this, C. fungigraminus mono-planting (MC), C. fungigraminus–potato interplanting (CIP) and C. fungigraminus–broad bean interplanting (CIB) were performed. Compared to MC, soil microbial biomass carbon (SMBC), soil organic matter (SOM), ammoniacal nitrogen (AMN), pH and soil amino sugars had a positive effect on the rhizosphere soil of CIP and CIB, as well as enhancing soil nitrogenase, nitrite reductase, and peroxidase activities (p < 0.05). Moreover, CIP improved the root vitality (2.08 times) and crude protein (1.11 times). In addition, CIB enhanced the crude fiber of C. fungigraminus seedlings. These two interplanting models also improved the microbial composition and diversity (Actinobacteria, Firmicutes, and Bacteroidota, etc.) in the rhizosphere soil of C. fungigraminus seedlings. Among all the samples, 189 and 59 genes were involved in methane cycling and nitrogen cycling, respectively, which improved the presence of the serine cycle, ribulose monophosphate, assimilatory nitrate reduction, methane absorption, and glutamate synthesis and inhibited denitrification. Through correlation analysis and the Mantel test, the putative functional genes, encoding functions in both nitrogen and methane cycling, were shown to have a significant positive effect on pH, moisture, AMN, SOM, SMBC, and soil peroxidase activity, while not displaying a significant effect on soil nitrogenase activity and total amino sugar (p < 0.05). The short-term influence of the interplanting model was shown to improve land use efficiency and economic profitability per unit land area, and the models could provide sustainable agricultural production for rural revitalization.
The Effects of Wind Velocity on the Binding Properties of Ash, Bottom Ash, and Additives: A Wind Tunnel Study
Authors: Petković Papalazarou Sandra , Nešković Jasmina , Ćorluka Stevan , Polavder Svetlana , Mitrašinović Aleksandar , Stjepanović Pavle
Published: August 2024

The more economically viable and environmentally sustainable approach for treating the by-products of coal combustion from thermal power plants entails their collective disposal as opposed to individual disposal methods. This aligns with pertinent EU directives and domestic regulations, ensuring compliance with established standards while optimizing resource utilization and minimizing environmental impact. This study evaluated the resistance to wind erosion of the binding properties of a mixture (fly ash (FA), bottom ash (BA), and additives) using an indoor wind tunnel under simulated ambient conditions. Investigations of the mutual impact of ash, bottom ash, and additives (CaO and Ca(OH)2) with a certain percentage of water were carried out with eighteen samples. The samples consisted of the water at six addition rates 5, 8, 10, 15, 20, and 25% (w/w), and additive at three addition rates (1, 2, and 3% (w/w). Based on the obtained results, the optimal ratios of additives (3% (w/w)) and water (15% (w/w)) were determined. Prior to the wind tunnel experiments, and according to the different addition rates of additives and water, eight samples were prepared with different addition rates of ash. The mass concentrations of suspended particles (PM10) and total suspended particles (TSPs) in these samples were measured at three distinct wind velocities: 1 m/s, 3 m/s, and 5 m/s, respectively. The results indicate that the samples containing the optimal content of additives and water demonstrate a maximum increase in PM10 emission zero values of no more than 1.9 times. This finding can be considered satisfactory from the standpoint of environmental protection.
Boosting the Activation of Molecular Oxygen and the Degradation of Rhodamine B in Polar-Functional-Group-Modified g-C3N4
Authors: Chen Jing , Yang Minghua , Zhang Hongjiao , Chen Yuxin , Ji Yujie , Yu Ruohan , Liu Zhenguo
Published: August 2024

Molecular oxygen activation often suffers from high energy consumption and low efficiency. Developing eco-friendly and effective photocatalysts remains a key challenge for advancing green molecular oxygen activation. Herein, graphitic carbon nitride (g-C3N4) with abundant hydroxyl groups (HCN) was synthesized to investigate the relationship between these polar groups and molecular oxygen activation. The advantage of the hydroxyl group modification of g-C3N4 included narrower interlayer distances, a larger specific surface area and improved hydrophilicity. Various photoelectronic measurements revealed that the introduced hydroxyl groups reduced the charge transfer resistance of HCN, resulting in accelerated charge separation and migration kinetics. Therefore, the optimal HCN-90 showed the highest activity for Rhodamine B photodegradation with a reaction time of 30 min and an apparent rate constant of 0.125 min−1, surpassing most other g-C3N4 composites. This enhanced activity was attributed to the adjusted band structure achieved through polar functional group modification. The modification of polar functional groups could alter the energy band structure of photocatalysts, narrow band gap, enhance visible-light absorption, and improve photogenerated carrier separation efficiency. This work highlights the significant potential of polar functional groups in tuning the structure of g-C3N4 to enhance efficient molecular oxygen activation.
Tuning Ferulic Acid Solubility in Choline-Chloride- and Betaine-Based Deep Eutectic Solvents: Experimental Determination and Machine Learning Modeling
Authors: Jeliński Tomasz , Przybyłek Maciej , Różalski Rafał , Romanek Karolina , Wielewski Daniel , Cysewski Piotr
Published: August 2024

Deep eutectic solvents (DES) represent a promising class of green solvents, offering particular utility in the extraction and development of new formulations of natural compounds such as ferulic acid (FA). The experimental phase of the study undertook a systematic investigation of the solubility of FA in DES, comprising choline chloride or betaine as hydrogen bond acceptors and six different polyols as hydrogen bond donors. The results demonstrated that solvents based on choline chloride were more effective than those based on betaine. The optimal ratio of hydrogen bond acceptors to donors was found to be 1:2 molar. The addition of water to the DES resulted in a notable enhancement in the solubility of FA. Among the polyols tested, triethylene glycol was the most effective. Hence, DES composed of choline chloride and triethylene glycol (TEG) (1:2) with added water in a 0.3 molar ration is suggested as an efficient alternative to traditional organic solvents like DMSO. In the second part of this report, the affinities of FA in saturated solutions were computed for solute-solute and all solute-solvent pairs. It was found that self-association of FA leads to a cyclic structure of the C28 type, common among carboxylic acids, which is the strongest type of FA affinity. On the other hand, among all hetero-molecular bi-complexes, the most stable is the FA-TEG pair, which is an interesting congruency with the high solubility of FA in TEG containing liquids. Finally, this work combined COSMO-RS modeling with machine learning for the development of a model predicting ferulic acid solubility in a wide range of solvents, including not only DES but also classical neat and binary mixtures. A machine learning protocol developed a highly accurate model for predicting FA solubility, significantly outperforming the COSMO-RS approach. Based on the obtained results, it is recommended to use the support vector regressor (SVR) for screening new dissolution media as it is not only accurate but also has sound generalization to new systems.
Exploring Diet-Based Treatments for Atrial Fibrillation: Patient Empowerment and Citizen Science as a Model for Quality-of-Life-Centered Solutions
Authors: Kuipers Myrthe F. , Laurila Ronja , Remy Maurice L. , van Oudheusden Michiel , Hazlett Nedra , Lipsky Sally , Reisner Lianna L. , McCall Debbe , de Groot Natasja M. S. , Brundel Bianca J. J. M.
Published: August 2024

Atrial fibrillation (AF) is the most common heart rhythm disorder in the Western world. Between the years 2010 and 2019, the global prevalence of AF rose from 33.5 million to 59 million, highlighting the importance of developing equitable treatments for patients. The disease is associated with symptoms such as palpitations, dizziness, fatigue, shortness of breath, and cognitive dysfunction. In addition, AF increases the risk of developing a stroke and heart failure. Despite new insights into risk factors that can lead to the development of AF, the success of current treatments is suboptimal. Numerous risk factors, such as hypertension, diabetes, and obesity, have been associated with the development and progression of AF. As these can be lifestyle-related risk factors, lifestyle modification may be a solution to reduce AF-related symptoms as well as episodes. Research results show that certain dietary changes can reduce AF and numerous risk factors for AF. Increasing attention is being given to Mediterranean and whole, plant-based eating patterns, which emphasize eating grains, legumes, vegetables, fruits, and nuts, while excluding most—or all—animal products. Hence, what are the beneficial aspects of a Mediterranean and plant-based diet which consists mainly of unprocessed foods? In the current review, we discuss the outcomes of diet-based treatments. Moreover, other diet-related treatments, brought up by patient initiatives, are highlighted. These patient-initiated studies include L-glutamine and electrolytes as options to manage AF. Also, we highlight the emerging importance of valuing patient needs and a quality-of-life-centered approach to medicine. As indicated by recent studies and patient experiences, citizen science can create inclusive solutions that lead to patient empowerment and a holistic approach for AF management.
Comparison of Glucose Metabolizing Properties of Enterobacterial Probiotic Strains In Vitro
Authors: Balanche Jules , Lahaye Emilie , Bremard Lisa , Thomas Benjamin , Fetissov Sergueï
Published: August 2024

Before the absorption in the intestine, glucose encounters gut bacteria, which may serve as a barrier against hyperglycemia by metabolizing glucose. In the present study, we compared the capacity of enterobacterial strains to lower glucose levels in an in vitro model of nutrient-induced bacterial growth. Two probiotic strains, Hafnia alvei HA4597 (H. alvei) and Escherichia coli (E. coli) Nissle 1917, as well as E. coli K12, were studied. To mimic bacterial growth in the gut, a planktonic culture was supplemented twice daily by the Luria Bertani milieu with or without 0.5% glucose. Repeated nutrient provision resulted in the incremental growth of bacteria. However, in the presence of glucose, the maximal growth of both strains of E. coli but not of H. alvei was inhibited. When glucose was added to the culture medium, a continuous decrease in its concentration was observed during each feeding phase. At its highest density, H. alvei displayed more efficient glucose consumption accompanied by a more pronounced downregulation of glucose transporters’ expression than E. coli K12. Thus, the study reveals that the probiotic strain H. alvei HA4597 is more resilient to maintain its growth than E. coli in the presence of 0.5% glucose accompanied by more efficient glucose consumption. This experimental approach offers a new strategy for the identification of probiotics with increased glucose metabolizing capacities potentially useful for the prevention and co-treatment of type 2 diabetes.
Missed Opportunities for HIV Diagnosis and Their Clinical Repercussions in the Portuguese Population—A Cohort Study
Authors: Lourinho João , Miguel Maria João , Gonçalves Frederico , Vale Francisco , Franco Cláudia Silva , Marques Nuno
Published: August 2024

Late human immunodeficiency virus (HIV) diagnosis has been associated with missed opportunities for earlier diagnosis. We conducted a retrospective, longitudinal, single-centre cohort study evaluating these missed opportunities and their clinical repercussions in adults with a new HIV diagnosis or who were drug-naïve, who attended our Infectious Diseases Department between 2018 and 2023. We assessed missed opportunities in the two years prior to diagnosis or after the last negative HIV test. We compared clinical and laboratorial data from individuals with and without missed opportunities. The primary outcome considered was AIDS-defining conditions at diagnosis. Among the 436 included individuals, 27.1% experienced at least one missed opportunity. Those with missed opportunities were more likely to be female (p = 0.007), older at their first consultation (p < 0.001), born in Africa (p < 0.001) and in countries with a high HIV prevalence (p < 0.001), and have heterosexual transmission (p < 0.001). The adjusted analysis showed that missed opportunities were significantly associated with AIDS-defining conditions at diagnosis (OR 3.23, CI 95% [1.62–6.46], p < 0.001). These findings highlight the impact of missed opportunities on HIV severity, underscoring the need for more targeted interventions to reduce them.
All-Analytic Statistical Modeling of Constellations in (Optical) Transmission Systems Driven by High-Speed Electronic Digital to Analog Converters Part I: DAC Mismatch Statistics, Metrics, Symmetries, Error Vector Magnitude
Authors: Nazarathy Moshe , Tomkos Ioannis
Published: August 2024

This two-part work develops a comprehensive toolbox for the statistical characterization of nonlinear distortions of DAC-generated signal constellations to be transmitted over communication links, be they electronic (wireline, wireless) or photonic, Mach–Zehnder modulator (MZM)-based optical interconnects in particular. The all-analytic toolbox developed here delivers closed-form expressions for the second-order statistics (means, variances) of all relevant constellation metrics of the DACs’ building blocks and of DAC-driven MZM-based optical transmitters, all the way to the slicer in the optical receivers over a linear channel with coherent detection. The key impairment targeted by the model is the random current mismatch of the ASIC devices implementing the DAC drivers. In particular the (skew-)centrosymmetry of the DAC metrics is formally derived and explored. A key applicative insight is that the conventional INL/DNL (Integral NonLinearity/Differential NonLinearity) constellation metrics, widely adopted in the electronic devices and circuits community, are not quite useful in the context of communication systems, since these metrics are ill-suited to predict communication link statistical performance. To rectify this deficiency of existing electronic DAC metrics, we introduce modified variants of the INL|DNL, namely the integral error vector (IEV) and the differential error vector (DEV) constellation metrics. The new IEV|DEV represent straightforward predictors of relevant communication-minded metrics: error vector magnitude (EVM) treated here in Part I, and Symbol/Bit Error-Rates (SER, BER) treated in the upcoming Part II of this paper.
Laser Scanning Method for Time-Resolved Measurements of Wavefront Distortion Introduced by Active Elements in High-Power Laser Amplifiers
Authors: Kuptsova Alyona O. , Kuptsov Gleb V. , Petrov Vladimir A. , Atuchin Victor V. , Petrov Victor V.
Published: August 2024

A novel method was proposed for the experimental investigation of wavefront distortion introduced to amplified radiation by pumped active elements in high-power laser amplifiers. The method is based on the simultaneous measurement of temperature distribution and the distribution of population density of the excited laser level in active elements. The underlying theory of the technique was presented; various factors affecting the accuracy of wavefront distortion determination were analyzed. The method was tested to study the wavefront distortion and the depolarization of radiation introduced by the Yb:YAG active element of a cryogenically cooled laser amplifier with high-power diode pumping. The focal length of the thermal lens was 0.40 ± 0.03 and 0.47 ± 0.05 m for the horizontal and vertical planes, respectively. The focal length of the electron lens was two orders of magnitude larger. The maximum value of losses induced by depolarization was 8.5%.
The Correction of Keystone Distortion in Czerny–Turner Spectrometer Using Freeform Surface
Authors: Xia Guo , Yu Defeng , Pan Qingfa , Pan Qin , Li Yanduo , Chen Xiaomeng
Published: August 2024

In the past, conventional Czerny–Turner spectrometers were usually designed to achieve high resolution while often ignoring astigmatism in the sagittal direction. In contrast, by replacing the focusing mirror with a freeform surface in the structure, we can obtain a Czerny–Turner spectrometer with low keystone distortion by controlling the astigmatism. At the same time, the area sensor can receive all of the spectrum from the optical system. In this paper, we briefly describe the formation of keystone distortion and smile in a plane grating. Additionally, the validity of the method is verified through simulation. Finally, we evaluated the smile and keystone distortion of both the initial and final systems. The keystone and smile were reduced to 1.77 μm and 8.3 μm, respectively, over the wavelength range of 535 nm to 630 nm. Concurrently, the resolution achieved was 0.4 nm.
Transcriptomic and Physiological Analyses for the Role of Hormones and Sugar in Axillary Bud Development of Wild Strawberry Stolon
Authors: Lan Genqian , Wu Mingzhao , Zhang Qihang , Yuan Bo , Shi Guangxin , Zhu Ni , Zheng Yibingyue , Cao Qiang , Qiao Qin , Zhang Ticao
Published: August 2024

Strawberries are mainly propagated by stolons, which can be divided into monopodial and sympodial types. Monopodial stolons consistently produce ramets at each node following the initial single dormant bud, whereas sympodial stolons develop a dormant bud before each ramet. Sympodial stolon encompasses both dormant buds and ramet buds, making it suitable for studying the formation mechanism of different stolon types. In this study, we utilized sympodial stolons from Fragaria nilgerrensis as materials and explored the mechanisms underlying sympodial stolon development through transcriptomic and phytohormonal analyses. The transcriptome results unveiled that auxin, cytokinin, and sugars likely act as main regulators. Endogenous hormone analysis revealed that the inactivation of auxin could influence bud dormancy. Exogenous cytokinin application primarily induced dormant buds to develop into secondary stolons, with the proportion of ramet formation being very low, less than 10%. Furthermore, weighted gene co-expression network analysis identified key genes involved in ramet formation, including auxin transport and response genes, the cytokinin activation gene LOG1, and glucose transport genes SWEET1 and SFP2. Consistently, in vitro cultivation experiments confirmed that glucose enhances the transition of dormant buds into ramets within two days. Collectively, cytokinin and glucose act as dormant breakers, with cytokinin mainly driving secondary stolon formation and glucose promoting ramet generation. This study improved our understanding of stolon patterning and bud development in the sympodial stolon of strawberries.
Genome-Wide Identification and Molecular Evolutionary History of the Whirly Family Genes in Brassica napus
Authors: Wang Long , Zhao Zhi , Li Huaxin , Pei Damei , Ma Qianru , Huang Zhen , Wang Hongyan , Xiao Lu
Published: August 2024

Whirly transcription factors are unique to plants, playing pivotal roles in managing leaf senescence and DNA repair. While present in various species, their identification in Brassica napus L. (B. napus) and their differences during hybridization and polyploidy has been elusive. Addressing this, our study delves into the functional and evolutionary aspects of the Whirly gene family during the emergence of B. napus, applying bioinformatics and comparative genomics. We identified six Whirly genes in B. napus. In Brassica rapa L. (B. rapa), three Whirly genes were identified, while four were found in Brassica oleracea L. (B. oleracea). The results show that the identified Whirly genes not only have homology but also share the same chromosomal positions. Phylogenetic analysis indicates that Whirly genes in monocots and dicots exhibit high conservation. In the evolutionary process, the Whirly gene family in B. napus experienced events of intron/exon loss. Collinearity insights point to intense purifying selection post-duplication. Promoter regions housed diverse cis-acting elements linked to photoresponse, anaerobic initiation, and methyl jasmonate responsiveness. Notably, elements tied to abscisic acid signaling and meristem expression were prominent in diploid ancestors but subdued in tetraploid B. napus. Tissue-specific expression unveiled analogous patterns within subfamily genes. Subsequent qRT-PCR analysis spotlighted BnAWHY1b’s potential significance in abiotic stress response, particularly drought. These findings can be used as theoretical foundations to understand the functions and effects of the Whirly gene family in B. napus, providing references for the molecular mechanism of gene evolution between this species and its diploid ancestors.
Antimicrobial Activity of Leaf Aqueous Extract of Schinus polygamus (Cav.) Cabrera against Pathogenic Bacteria and Spoilage Yeasts
Authors: Acuña-Fontecilla Andrea , Bruna Julio , Ganga María Angélica , Godoy Liliana
Published: August 2024

The antimicrobial activity of an aqueous extract of the leaves of Schinus polygamus (cav.) Cabrera against microorganisms of food importance was evaluated. First, the leaf aqueous extract of Schinus polygamus was characterized, quantifying hydroxycinnamic acids and phenolic compounds. Then, a battery of strains was tested, including Escherichia coli ATCC 25922, Salmonella Typhimurium ATCC 14028, and Listeria monocytogenes ATCC 13932. Also, we tested wine spoilage yeasts such as Brettanomyces bruxellensis LAMAP2480, B. bruxellensis LAMAP1359, B. bruxellensis CECT1451, and Pichia guilliermondii NPCC1051. Tests were conducted using the kinetic curve of growth and cell viability counts. The results indicate that with 10% v/v of concentrated extract, it is possible to observe growth inhibition of all microorganisms studied, with statistically significant differences during the whole measurement time (70 h for bacteria and 145 h for yeast).
The Invasive Plant Amaranthus spinosus L. Exhibits a Stronger Resistance to Drought than the Native Plant A. tricolor L. under Co-Cultivation Conditions When Treated with Light Drought
Authors: Wang Congyan , Liu Yingsheng , Li Chuang , Li Yue , Du Daolin
Published: August 2024

Drought may facilitate the invasion process of invasive plants, mainly because invasive plants can obtain a stronger growth competitiveness than native plants under drought. It is therefore imperative to illuminate the mechanisms underlying the successful invasion of invasive plants under drought, with a particular focus on the differences in the resistance of invasive and native plants to drought. This study aimed to elucidate the differences in the resistance between the invasive plant Amaranthus spinosus L. and the native plant A. tricolor L. to drought under a gradient of drought. The resistance of co-cultivated A. spinosus to drought was significantly higher than that of co-cultivated A. tricolor under light drought. Hence, A. spinosus may obtain a stronger competitive advantage than A. spinosus under co-cultivation conditions when treated with light drought. The resistance of the two plants to drought may be predominantly influenced by their height and biomass. This present study also defines a method for evaluating the stress resistance of a given plant species to stress by calculating the stress resistance index. This present study offers a robust theoretical foundation for determining the stress resistance of a given plant species and the environmental management of A. spinosus under drought.
Predicting the Adhesive Layer Thickness in Hybrid Joints Involving Pre-Tensioned Bolts
Authors: Ricca Frederico , Galindo-Rosales Francisco J. , Akhavan-Safar Alireza , da Silva Lucas F. M. , Fkyerat Thomas , Yokozeki Koichi , Vallée Till , Evers Tobias
Published: August 2024

While most academic studies focus on the properties of cured joints, this research addresses the manufacturing process of hybrid joints in their uncured state. Hybrid joints that combine adhesive bonding with pre-tensioned bolts exhibit superior mechanical performance compared to exclusively bonded or bolted joints. However, the adhesive flow during manufacturing in hybrid joints often results in a nonuniform adhesive thickness, where obtaining an exact thickness is crucial for accurate load capacity predictions. This paper presents experiments involving three different adhesives, providing precise measurements of the adhesive layer thickness distribution, which served as a reference when evaluating and validating the subsequent numerical predictions. The numerical predictions were performed using computational fluid dynamics (CFD) to model the flow behavior of the adhesives during the bonding process and their interactions with the metal substrates. The CFD predictions of the adhesive layer thickness showed good agreement with the experimental data, with the relative differences between the average experimental and numerical thickness values ranging from 4.07% to 27.1%. The results were most accurate for the adhesive with sand particles, whose particles remained intact, ensuring that the adhesive’s rheology remained unchanged. The results highlight the importance of the rheological behavior of the adhesive in the final distribution of the adhesive layer thickness, thereby expanding the understanding of these joints.