bluuebunny/crossref_metadata_2025 huggingface dataset:
Severe Muscle Deconditioning Triggers Early Extracellular Matrix Remodeling and Resident Stem Cell Differentiation into Adipocytes in Healthy Men
Authors: Guilhot Corentin , Fovet Théo , Delobel Pierre , Dargegen Manon , Jasmin Bernard J. , Brioche Thomas , Chopard Angèle , Py Guillaume
Published: May 2022

Besides the loss of muscle mass and strength, increased intermuscular adipose tissue (IMAT) is now a well-recognized consequence of muscle deconditioning as experienced in prolonged microgravity. IMAT content may alter the muscle stem cell microenvironment. We hypothesized that extracellular matrix structure alterations and microenvironment remodeling induced by fast and severe muscle disuse could modulate fibro-adipogenic progenitor fate and behavior. We used the dry immersion (DI) model that rapidly leads to severe muscle deconditioning due to drastic hypoactivity. We randomly assigned healthy volunteers (n = 18 men) to the control group (only DI, n = 9; age = 33.8 ± 4) or to the DI + thigh cuff group (n = 9; age = 33.4 ± 7). Participants remained immersed in the supine position in a thermo-neutral water bath for 5 days. We collected vastus lateralis biopsies before (baseline) and after DI. 5 days of DI are sufficient to reduce muscle mass significantly, as indicated by the decreased myofiber cross-sectional area in vastus lateralis samples (−18% vs. baseline, p < 0.05). Early and late adipogenic differentiation transcription factors protein levels were upregulated. Platelet-derived growth Factors alpha (PDGFR⍺) protein level and PDGFR⍺-positive cells were increased after 5 days of DI. Extracellular matrix structure was prone to remodeling with an altered ECM composition with 4 major collagens, fibronectin, and Connective Tissue Growth Factor mRNA decreases (p < 0.001 vs. baseline). Wearing thigh cuffs did not have any preventive effect on the measured variable. Our results show that altered extracellular matrix structure and signaling pathways occur early during DI, a severe muscle wasting model, favoring fibro-adipogenic progenitor differentiation into adipocytes.
One of the Primary Functions of Tissue-Resident Pluripotent Pericytes Cells May Be to Regulate Normal Organ Growth and Maturation: Implications for Attempts to Repair Tissues Later in Life
Authors: Hart David A.
Published: May 2022

Adult mesenchymal stem cells were reported more than 30 years ago. Since then, their potential to repair and regenerate damaged or diseased tissues has been studied intensively in both preclinical models and human trials. Most of the need for such tissue repair/regeneration is in older populations, so much of the effort has been performed with autologous cells in older patients. However, success has been difficult to achieve. In the literature, it has been noted that such progenitor cells from younger individuals often behave with more vigorous activity and are functionally enhanced compared to those from older individuals or animals. In addition, cells with the characteristics of mesenchymal stem cells or pluripotent mesenchymal regulatory cells exist in nearly all tissues and organs as pericytes since fetal life. Such evidence raises the possibility that one of the primary roles of these organ-specific cells is to regulate organ growth and maturation, and then subsequently play a role in the maintenance of organ integrity. This review will discuss the evidence to support this concept and the implications of such a concept regarding the use of these progenitor cells for the repair and regeneration of tissues damaged by injury or disease later in life. For the latter, it may be necessary to return the organ-specific progenitor cells to the functional state that contributed to their effectiveness during growth and maturation rather than attempting to use them after alterations imposed during the aging process have been established and their function compromised.
Human Peripheral Blood Mononucleocyte Derived Myeloid Committed Progenitor Cells Mitigate H-ARS by Exosomal Paracrine Signal
Authors: Chugh Rishi Man , Bhanja Payel , Olea Ximena Diaz , Tao Fang , Schroeder Kealan , Zitter Ryan , Arora Tanu , Pathak Harsh , Kimler Bruce F. , Godwin Andrew K. , Perry John M. , Saha Subhrajit
Published: May 2022

Radiation-induced loss of the hematopoietic stem cell progenitor population compromises bone marrow regeneration and development of mature blood cells. Failure to rescue bone marrow functions results in fatal consequences from hematopoietic injury, systemic infections, and sepsis. So far, bone marrow transplant is the only effective option, which partially minimizes radiation-induced hematopoietic toxicities. However, a bone marrow transplant will require HLA matching, which will not be feasible in large casualty settings such as a nuclear accident or an act of terrorism. In this study we demonstrated that human peripheral blood mononuclear cell-derived myeloid committed progenitor cells can mitigate radiation-induced bone marrow toxicity and improve survival in mice. These cells can rescue the recipient’s hematopoietic stem cells from radiation toxicity even when administered up to 24 h after radiation exposure and can be subjected to allogenic transplant without GVHD development. Transplanted cells deliver sEVs enriched with regenerative and immune-modulatory paracrine signals to mitigate radiation-induced hematopoietic toxicity. This provides a natural polypharmacy solution against a complex injury process. In summary, myeloid committed progenitor cells can be prepared from blood cells as an off-the-shelf alternative to invasive bone marrow harvesting and can be administered in an allogenic setting to mitigate hematopoietic acute radiation syndrome.
Impaired LEF1 Activation Accelerates iPSC-Derived Keratinocytes Differentiation in Hutchinson-Gilford Progeria Syndrome
Authors: Mao Xiaojing , Xiong Zheng-Mei , Xue Huijing , Brown Markus A. , Gete Yantenew G. , Yu Reynold , Sun Linlin , Cao Kan
Published: May 2022

Hutchinson–Gilford progeria syndrome (HGPS) is a detrimental premature aging disease caused by a point mutation in the human LMNA gene. This mutation results in the abnormal accumulation of a truncated pre-lamin A protein called progerin. Among the drastically accelerated signs of aging in HGPS patients, severe skin phenotypes such as alopecia and sclerotic skins always develop with the disease progression. Here, we studied the HGPS molecular mechanisms focusing on early skin development by differentiating patient-derived induced pluripotent stem cells (iPSCs) to a keratinocyte lineage. Interestingly, HGPS iPSCs showed an accelerated commitment to the keratinocyte lineage than the normal control. To study potential signaling pathways that accelerated skin development in HGPS, we investigated the WNT pathway components during HGPS iPSCs-keratinocytes induction. Surprisingly, despite the unaffected β-catenin activity, the expression of a critical WNT transcription factor LEF1 was diminished from an early stage in HGPS iPSCs-keratinocytes differentiation. A chromatin immunoprecipitation (ChIP) experiment further revealed strong bindings of LEF1 to the early-stage epithelial developmental markers K8 and K18 and that the LEF1 silencing by siRNA down-regulates the K8/K18 transcription. During the iPSCs-keratinocytes differentiation, correction of HGPS mutation by Adenine base editing (ABE), while in a partial level, rescued the phenotypes for accelerated keratinocyte lineage-commitment. ABE also reduced the cell death in HGPS iPSCs-derived keratinocytes. These findings brought new insight into the molecular basis and therapeutic application for the skin abnormalities in HGPS.
Novel All-Nitrogen Molecular Crystals Composed of Tetragonal N4 Molecules
Authors: Pang Suna , Wang Feng
Published: May 2022

A computational study promises insight into molecular crystals consisting of the tetrahedral form of N4 molecules (Td-N4). Here, our efforts are focused on theoretically predicting the existence of the molecular crystals consisting of Td-N4 molecules. On the basis of the first principles of Born–Oppenheimer molecular dynamics under constant temperature and pressure, and geometry optimizations under hydrostatic pressures without any constrained parameters, molecular crystals consisting of Td-N4 molecules were confirmed to be dynamically and thermally metastable. Our analysis shows that, with high detonation performance and high stability, these Td-N4 molecular crystals can indeed be potential candidates as high-energy density explosives.
Platelet Extracellular Vesicles Are Taken up by Canine T Lymphocytes but Do Not Play a Role in Their Proliferation, Differentiation and Cytokine Production In Vitro
Authors: Żmigrodzka Magdalena , Witkowska-Piłaszewicz Olga , Pingwara Rafał , Winnicka Anna
Published: May 2022

Eukaryotic and prokaryotic cells in physiological and pathological conditions form membrane-bound extracellular vesicles, known as EVs. The ability of these submicron structures to transfer their cargoes (miRNA, DNA, protein, cytokines, receptors, etc.) into recipient cells is described. Recent data revealed that platelet-derived extracellular vesicles (PEVs) crosstalk promotes cancer growth and metastasis formation. Moreover, they exert immunosuppressive activities on phagocytes. This EV subpopulation is the most abundant amongst all types in circulation. According to the authors’ best knowledge, there is no information regarding the impact of PEVs on canine lymphocytes. The aim of this study was to evaluate the influence of PEVs on lymphocyte proliferation, phenotype and cytokine production in vitro. In the study, it was demonstrated (i) that PEVs interact differently with lymphocyte subsets and are preferentially associated with T-lymphocytes PBMC, while (ii) they are rarely detected in association with B-lymphocytes, and there is evidence that (iii) PEV uptake is observed after 7 h of co-culturing with lymphocytes. In addition, obtained data support the notion that PEVs do not influence in vitro lymphocyte proliferation, differentiation and cytokine production in a canine model.
A Novel Method of Endotoxins Removal from Chitosan Hydrogel as a Potential Bioink Component Obtained by CO2 Saturation
Authors: Banach-Kopeć Adrianna , Mania Szymon , Pilch Joanna , Augustin Ewa , Gabriel Iwona , Tylingo Robert
Published: May 2022

The article presents a new approach in the purification of chitosan (CS) hydrogel in order to remove a significant amount of endotoxins without changing its molecular weight and viscosity. Two variants of the method used to purify CS hydrogels from endotoxins were investigated using the PyroGene rFC Enzymatic Cascade assay kit. The effect of the CS purification method was assessed in terms of changes in the dynamic viscosity of its hydrogels, the molecular weight of the polymer, microbiological purity after refrigerated storage and cytotoxicity against L929 cells based on the ISO 10993-5:2009(E) standard. The proposed purification method 1 (M1) allows for the removal of significant amounts of endotoxins: 87.9–97.6% in relation to their initial concentration in the CS hydrogel without affecting the solution viscosity. Moreover, the final solutions were sterile and microbiologically stable during storage. The M1 purification method did not change the morphology of the L929 cells.
CD26 Deficiency Controls Macrophage Polarization Markers and Signal Transducers during Colitis Development and Resolution
Authors: Vukelic Iva , Buljevic Suncica , Baticic Lara , Barisic Karmela , Franovic Barbara , Detel Dijana
Published: May 2022

Ulcerative colitis (UC) is a multifactorial condition characterized by a destructive immune response that failed to be attenuated by common regulatory mechanisms which reduce inflammation and promote mucosa healing. The inhibition of CD26, a multifunctional glycoprotein that controls the immune response via its dipeptidyl peptidase (DP) 4 enzyme activity, was proven to have beneficial effects in various autoimmune inflammatory diseases. The polarization of macrophages into either pro-inflammatory M1 or anti-inflammatory M2 subclass is a key intersection that mediates the immune-inflammatory process in UC. Hence, we hypothesized that the deficiency of CD26 affects that process in the dextran sulfate sodium (DSS)-induced model of UC. We found that mRNA expression of M2 markers arginase 1 and Fizz were increased, while the expression of M1 marker inducible NO synthase was downregulated in CD26−/− mice. Decreased STAT1 mRNA, as well as upregulated pSTAT6 and pSTAT3, additionally support the demonstrated activation of M2 macrophages under CD26 deficiency. Finally, we investigated DP8 and DP9, proteins with DP4-like activity, and found that CD26 deficiency is not a key factor for the noted upregulation of their expression in UC. In conclusion, we demonstrate that CD26 deficiency regulates macrophage polarization toward the anti-inflammatory M2 phenotype, which is driven by STAT6/STAT3 signaling pathways. This process is additionally enhanced by the reduction of M1 differentiation via the suppression of proinflammatory STAT1. Therefore, further studies should investigate the clinical potential of CD26 inhibitors in the treatment of UC.
Machine Learning Methods in CFD for Turbomachinery: A Review
Authors: Hammond James , Pepper Nick , Montomoli Francesco , Michelassi Vittorio
Published: May 2022

Computational Fluid Dynamics is one of the most relied upon tools in the design and analysis of components in turbomachines. From the propulsion fan at the inlet, through the compressor and combustion sections, to the turbines at the outlet, CFD is used to perform fluid flow and heat transfer analyses to help designers extract the highest performance out of each component. In some cases, such as the design point performance of the axial compressor, current methods are capable of delivering good predictive accuracy. However, many areas require improved methods to give reliable predictions in order for the relevant design spaces to be further explored with confidence. This paper illustrates recent developments in CFD for turbomachinery which make use of machine learning techniques to augment prediction accuracy, speed up prediction times, analyse and manage uncertainty and reconcile simulations with available data. Such techniques facilitate faster and more robust searches of the design space, with or without the help of optimization methods, and enable innovative designs which keep pace with the demand for improved efficiency and sustainability as well as parts and asset operation cost reduction.
A Study of Inbound Travelers Experience and Satisfaction at Quarantine Hotels in Indonesia during the COVID-19 Pandemic
Authors: Handani Narariya Dita , Riswanto Aura Lydia , Kim Hak-Seon
Published: May 2022

The tourism and hospitality sectors contribute significantly to the Indonesian economy. Meanwhile, COVID-19 affects these sectors. During the pandemic, the Indonesian government applied quarantine regulations at designated hotels to support its tourism industry. Since COVID-19 is becoming endemic and travel bans are being relaxed, hotel satisfaction becomes a crucial factor in quarantine hotels. If guests have a positive experience while staying at these hotels, they are likely to return for a staycation or vacation in the near future. The study examined 4856 reviews from Google reviews on 15 quarantine hotels in Indonesia. Following word frequency calculations in a matrix, UCINET 6.0 is used to analyze the network centrality and perform CONCOR analysis. The CONCOR analysis categorizes the review data into five categories. As quantitative analysis was performed, exploratory factor analysis was grouped into six variables: tangible, assurance, frontline, accommodation, quarantine, and location. As a result, tangible, assurance, and frontline negatively impacted guest satisfaction. Furthermore, three other variables: accommodation, quarantine, location, which have a positive influence, will lead to increased trust from inbound travelers. For managerial implication, results allow managers of quarantine hotels in Indonesia to focus more on improving tangible, assurance, and frontline factors.
Functionalization of Porphyrins Using Metal-Catalyzed C–H Activation
Authors: Kiselev Aleksey N. , Syrbu Sergey A. , Lebedeva Natalia Sh. , Gubarev Yury A.
Published: May 2022

The review is devoted to the C–H functionalization of porphyrins. Porphyrins exhibit the properties of organic semiconductors, light energy converters, chemical and electrochemical catalysts, and photocatalysts. The review describes the iridium- and palladium-catalyzed direct functionalization of porphyrins, with more attention given to the results obtained in our laboratory. The development and improvement of synthetic methods that do not require preliminary modification of the substrate with various functional groups are extremely important for the preparation of new organic materials based on porphyrins. This makes it possible to simplify the synthetic procedure, to make the synthesis more economical, environmentally safe, and simple to perform.
The Century Old Taxonomic Confusion Surrounding Wiedemannia zetterstedti Fallén and Related Species Is Resolved (Diptera: Empididae): Revision of the W. zetterstedti Group
Authors: Ivković Marija , Sinclair Bradley J. , Wahlberg Emma
Published: May 2022

The Wiedemannia zetterstedti species group is revised after examination of all available type specimens and includes one new species (W. ulrichi Ivković & Sinclair sp. nov.) and four redescribed species (W. czernyi (Bezzi), W. longipennis (Mik) stat. rev., W. rufipes (Oldenberg) stat. rev. and W. zetterstedti (Fallén)). The following new synonyms are proposed: W. (Roederella) ouedorum Vaillant, 1952 = W. czernyi (Bezzi, 1905); Paramesia riparia Robert, 1836 = W. zetterstedti (Fallén, 1826). Lectotypes are designated for the following species/subspecies: Atalanta hirtiloba Speiser, Brachystoma escheri Zetterstedt, Clinocera czernyi Bezzi, Clinocera longipennis Mik, Paramesia riparia Robert, and Roederia czernyi rufipes Oldenberg. In addition to morphological evidence, molecular species concepts were investigated using a molecular phylogenetic divergence-based species delimitation (bPTP) and results confirmed the morphological conclusions. A key to species is presented and geographic distributions are mapped.
A Chironomid Record of Early-Middle Holocene Environmental Evolution in the Darhad Basin, Northern Mongolia
Authors: Ni Zhenyu , Zhang Enlou , Yi Sangheon , Sun Weiwei , Meng Xianqiang , Ning Dongliang , Kim Jin Cheul
Published: May 2022

Under the influence of various circulation systems, the Holocene humidity conditions on the Mongolian Plateau are spatially heterogeneous and the underlying mechanism is still ambiguous. The complexity of climate change may affect the accuracy of assessing lake ecosystem evolution. In this study, based on the precise chronology, a chironomid assemblage sequence from the Darhad Basin in northern Mongolia is analyzed to elucidate the hydroclimate variation during the early-middle Holocene. The results show that the chironomid communities changed suddenly from littoral taxa to sublittoral/profundal taxa at about 9 cal kyr BP, reflecting an environmental transition from a river or shallow lake condition to a deep lake environment. Thereafter, most parts of the paleolake remained at a relatively high level until 4.5 cal kyr BP. This hydrological pattern resembles the typical humidity variations in the Westerlies affected regions, except that the onset of wetter conditions occurred one thousand years earlier as reflected in our results. The melting of glaciers and permafrost in the basin resulting from the early increased summer solar insolation could be a feasible explanation for these time advances.
Practical Approaches to Build and Sustain a Cardio-Oncology Clinic
Authors: Chasouraki Angeliki , Kourek Christos , Sianis Alexandros , Loritis Konstantinos , Kostakou Peggy , Tsougos Elias , Paraskevaidis Ioannis , Ntalianis Argyrios , Briasoulis Alexandros
Published: May 2022

The therapeutical advances in recent years in the field of oncology treatment have increased survival rates and improved the quality of life of oncology patients, thus turning cancer into a chronic disease. However, most of the new cancer treatments come at the expense of serious cardiovascular adverse events threatening the success story of these patients. The establishment of multidisciplinary medical teams to prevent, monitor, and treat cardiovascular diseases in cancer-treated patients is needed now more than ever. The aim of this narrative review is to demonstrate the existing knowledge and practical approaches on how to establish and maintain a cardio-oncology program for the rising number of patients who need it.
Cognitive–Behavioral Treatment of Obsessive–Compulsive Disorder: The Results of a Naturalistic Outcomes Study
Authors: Gragnani Andrea , Zaccari Vittoria , Femia Giuseppe , Pellegrini Valerio , Tenore Katia , Fadda Stefania , Luppino Olga , Basile Barbara , Cosentino Teresa , Perdighe Claudia , Romano Giuseppe , Saliani Angelo , Mancini Francesco
Published: May 2022

Cognitive–behavioral therapy is a well-established treatment for obsessive–compulsive disorder (OCD). There are a variety of cognitive and behavioral strategies, and it is necessary to analyze the outcomes of the treatments. The aim of the present study is to verify the effectiveness of a treatment that combines evidence-based procedures and specific cognitive interventions highlighting the issue of acceptance. Forty patients with OCD were recruited and underwent a specific treatment procedure. All patients had a psychodiagnostic assessment for OCD using the Y–BOCS (Yale–Brown obsessive–compulsive scale) performed twice: before treatment (t0) and after nine months (t1). Data analysis showed a decrease in the scores between t0 and t1 according to the Y–BOCS in terms of the interference, severity, and impairment of obsessive–compulsive symptoms. A repeated-measures ANOVA showed a significant reduction in symptoms after treatment, with values of F (1, 39) = 137.56, p < 0.001, and η2 = 0.78. The ANOVA results were corroborated by a Wilcoxon signed-rank test. A reliable change index analysis indicated that 33 participants reported improvements in symptoms, of which 23 were clinically significant. The results showed clinical relevance for OCD treatment and highlighted how this cognitive procedure favored positive outcomes.
Kinematic Parameters Following Pilon Fracture Treatment with the Ilizarov Method
Authors: Wietecki Paweł , Pawik Łukasz , Fink-Lwow Felicja , Leśkow Artur , Górski Radosław , Pawik Malwina , Olech Jarosław , Klepacki Krzysztof , Kuliński Patryk , Reichert Paweł , Morasiewicz Piotr
Published: May 2022

Background: The purpose of our study was to analyze kinematic parameters following pilon fracture treatment with the Ilizarov method. Methods: Our study assessed kinematic parameters of gait in 23 patients with pilon fractures treated with the Ilizarov method. Patients had completed their treatment 24–48 months prior to measurements. The range-of-motion values in the non-operated limb (NOL) and operated limb (OL) were compared. Kinematic parameters were measured using the Noraxon MyoMOTION System. Results: We observed no significant differences in hip flexion, hip abduction, or knee flection between the OLs and NOLs in patients after treatment with the Ilizarov method. We observed significant differences in the ranges of ankle dorsiflexion, inversion, and abduction (p < 0.001; p < 0.001; p < 0.003, respectively) between the OLs and the NOLs. Conclusion: Following pilon fracture treatment with the Ilizarov method, we observed no differences in terms of knee or hip joint mobility between the OL and the NOL, whereas the range of motion in the ankle joint of the OL was significantly limited. The treatment of pilon fractures with the Ilizarov method does not ensure the complete normalization of ankle joint kinematic parameters. Therefore, intense personalized rehabilitation of the ankle joint is recommended.
The Feasibility of the Functional Listening Index—Paediatric (FLI-P®) for Young Children with Hearing Loss
Authors: Davis Aleisha , Harrison Elisabeth , Cowan Robert
Published: May 2022

(1) Background: There is clear evidence supporting the need for individualized early intervention in children with hearing loss. However, relying on hearing thresholds and speech and language test results to guide intervention alone is problematic, particularly in infants and young children. This study aimed to establish the feasibility of a tool to monitor the development of functional listening skills to inform early and ongoing decisions by parents and professionals. (2) Methods: The FLI-P® is a 64-item checklist completed by parents and/or a child’s team. The listening development of 543 children with hearing loss enrolled in an early intervention and cochlear implant program was tracked with the FLI-P over a 6-year period. The scores for individual children were grouped according to hearing loss, device, additional needs, and age at device fitting. (3) Results: Results indicate that the FLI-P is a feasible and viable clinical measure that can be used to identify and track a child’s developing listening skills. Its use across a wide range of children supports its broad application. Children’s individual scores and aggregated group data were consistent with indicated expected differences and variations. Children’s individual scores and aggregated group data indicated expected differences and variations. (4) Conclusions: Information provided by children’s listening scores on the FLI-P can guide and support discussions and intervention decisions and bridge the gap between information from audiological assessments and language measures.
Is It Inflammatory Bowel Disease Flare or Pediatric Inflammatory Multisystem Syndrome Temporally Associated with COVID-19?
Authors: Krawiec Paulina , Opoka-Winiarska Violetta , Pac-Kożuchowska Elżbieta
Published: May 2022

Background: Pediatric inflammatory multisystem syndrome temporally associated with COVID-19/multi-system inflammatory syndrome in children (PIMS-TS/MIS-C) is a potentially life-threatening complication of SARS-CoV-2 infection in children. Gastrointestinal manifestations are prominent in children with PIMS-TS/MIS-C. Thus, it is challenging to differentiate this condition from an exacerbation of inflammatory bowel disease (IBD). We aimed to present the clinical characteristics, and diagnostic and therapeutic difficulties in patients with overlapping IBD and PIMS-TS/MIS-C; Methods: We reviewed medical records of children hospitalized due to overlapping IBD and PIMS-TS/MIS-C in a single pediatric hospital from December 2020 to December 2021; Results: There were four children with overlapping IBD flare and PIMS-TS/MIS-C. In three cases, IBD recognition preceded PIMS-TS/MIS-C onset and PIMS-TS/MIS-C occurred during anti-inflammatory therapy of IBD. All children presented with gastrointestinal symptoms at PIMS-TS/MIS-C onset. All patients received IVIG and ASA treatment. In three children there was a need to use steroids to resolve PIMS-TS/MIS-C symptoms. One child was vaccinated against COVID-19; Conclusions: SARS-CoV-2 infection may affect patients with underlying inflammatory conditions such as IBD, inducing systemic symptoms of PIMS-TS/MIS-C, and probably triggering IBD after PIMS-TS/MIS-C. The resemblance of clinical presentations is the main source of diagnostic and therapeutic challenges in PIMS-TS/MIS-C in patients with underlying IBD.
Cardiorespiratory Adaptation to Short-Term Exposure to Altitude vs. Normobaric Hypoxia in Patients with Pulmonary Hypertension
Authors: Schneider Simon , Lichtblau Mona , Furian Michael , Mayer Laura , Berlier Charlotte , Müller Julian , Saxer Stéphanie , Schwarz Esther , Bloch Konrad , Ulrich Silvia
Published: May 2022

Prediction of adverse health effects at altitude or during air travel is relevant, particularly in pre-existing cardiopulmonary disease such as pulmonary arterial or chronic thromboembolic pulmonary hypertension (PAH/CTEPH, PH). A total of 21 stable PH-patients (64 ± 15 y, 10 female, 12/9 PAH/CTEPH) were examined by pulse oximetry, arterial blood gas analysis and echocardiography during exposure to normobaric hypoxia (NH) (FiO2 15% ≈ 2500 m simulated altitude, data partly published) at low altitude and, on a separate day, at hypobaric hypoxia (HH, 2500 m) within 20–30 min after arrival. We compared changes in blood oxygenation and estimated pulmonary artery pressure in lowlanders with PH during high altitude simulation testing (HAST, NH) with changes in response to HH. During NH, 4/21 desaturated to SpO2 < 85% corresponding to a positive HAST according to BTS-recommendations and 12 qualified for oxygen at altitude according to low SpO2 < 92% at baseline. At HH, 3/21 received oxygen due to safety criteria (SpO2 < 80% for >30 min), of which two were HAST-negative. During HH vs. NH, patients had a (mean ± SE) significantly lower PaCO2 4.4 ± 0.1 vs. 4.9 ± 0.1 kPa, mean difference (95% CI) −0.5 kPa (−0.7 to −0.3), PaO2 6.7 ± 0.2 vs. 8.1 ± 0.2 kPa, −1.3 kPa (−1.9 to −0.8) and higher tricuspid regurgitation pressure gradient 55 ± 4 vs. 45 ± 4 mmHg, 10 mmHg (3 to 17), all p < 0.05. No serious adverse events occurred. In patients with PH, short-term exposure to altitude of 2500 m induced more pronounced hypoxemia, hypocapnia and pulmonary hemodynamic changes compared to NH during HAST despite similar exposure times and PiO2. Therefore, the use of HAST to predict physiological changes at altitude remains questionable. (ClinicalTrials.gov: NCT03592927 and NCT03637153).
Cardiopulmonary Exercise Testing and Cardiac Biomarker Measurements in Young Football Players: A Pilot Study
Authors: Costache Alexandru-Dan , Roca Mihai , Honceriu Cezar , Costache Irina-Iuliana , Leon-Constantin Maria-Magdalena , Mitu Ovidiu , Miftode Radu-Ștefan , Maștaleru Alexandra , Iliescu-Halițchi Dan , Halițchi-Iliescu Codruța-Olimpiada , Ion Adriana , Duca Ștefania-Teodora , Popa Delia-Melania , Abălasei Beatrice , Mocanu Veronica , Mitu Florin
Published: May 2022

Constant and intense physical activity causes physiological adaptive changes in the human body, but it can also become a trigger for adverse events, such as sudden cardiac arrest or sudden cardiac death. Our main objective was to assess the use of combined cardiopulmonary exercise testing (CPET) and cardiac biomarker determinants in young professional athletes. We conducted a study which involved the full examination of 19 football players, all male, aged between 18 and 20 years old. They underwent standard clinical and paraclinical evaluation, a 12-lead electrocardiogram (ECG), and transthoracic echocardiography (TTE). Afterwards, a tailored CPET was performed and peripheral venous blood samples were taken before and 3 h after the test in order to determine five biomarker levels at rest and post-effort. The measured biomarkers were cardiac troponin I (cTnI), myoglobin (Myo), the MB isoenzyme of creatine-kinase (CK-MB), the N-terminal prohormone of brain natriuretic peptide (NT-proBNP) and D-dimers. While cTnI and NT-proBNP levels were undetectable both at rest and post-effort in all subjects, the variations in Myo, CK-MB and D-dimers showed significant correlations with CPET parameters. This highlights the potential use of combined CPET and biomarker determinants to evaluate professional athletes, and encourages further research on larger study groups.
Design and Validation of a Scale of Knowledge of Cardiovascular Risk Factors and Lifestyle after Coronary Event
Authors: Bernal-Jiménez María Ángeles , Calle-Pérez Germán , Gutiérrez-Barrios Alejandro , Gheorghe Livia , Solano-Mulero Ana María , Trujillo-Garrido Nuria , Rodríguez-Martín Amelia , Tur Josep A. , Vázquez-García Rafael , Santi-Cano María José
Published: May 2022

Background: It is important for health professionals to have tools available to assess patients’ knowledge of lifestyle and cardiovascular risk factors after they have suffered a coronary event and determine whether educational interventions are effective. This study aims to design and validate a scale to evaluate this knowledge. Methods: Four-phase instrument design: (A) Conceptual review. (B) Review by experts. (C) Pilot test–retest. (D) Psychometric validation of the final version of the questionnaire with 24 items. A panel of experts performed the content validity. The reliability of the scale was measured using Cronbach’s alpha score and criterion validity was evaluated by comparing the total scores for knowledge obtained by the participants among the three education level groups. The construct and dimensional structure validity were assessed using exploratory factor analysis. Results: A total of 143 people participated, 30 in the pilot study and 113 (68% male, 60.2 ± 9 years) in the psychometric validation of version 3 of the scale. A Cronbach’s alpha score of 0.887 was reached for this version. The factor analysis showed that the items were distributed into five factors that explained 57% of the variance. Significant differences were observed in the level of knowledge among the patients of the three levels of education (low, moderate and high) (99.20 ± 11.93, 105.92 ± 7.85, 109.78 ± 8.76 points, p = 0.003), as there was a negative correlation between age and knowledge level (r = −0.213, p = 0.024). Conclusions: The scale presents psychometric properties that are evidence of its reliability and validity. The relationship demonstrated between the level of knowledge and age, sex and level of education shows the importance of emphasizing educational interventions for elderly people and those with a lower level of education.
Increased Risk of Hypothyroidism in People with Asthma: Evidence from a Real-World Population-Based Study
Authors: Huang Shih-Cheng , Gau Shuo-Yan , Huang Jing-Yang , Wu Wen-Jun , Wei James Cheng-Chung
Published: May 2022

Background: Non-T2 asthma and hypothyroidism share several inflammatory mechanisms in common. However, large-scale, real-world studies evaluating the association between asthma and hypothyroidism are lacking. The objective of this study was to evaluate the risk for asthma patients of developing hypothyroidism. Methods: In the retrospective cohort study, people with asthma were recruited from the Longitudinal Health Insurance Database in Taiwan. After excluding ineligible patients with a previous history of hypothyroidism, 1:1 propensity matching was conducted to select a non-asthma control group. Based on the multivariate Cox regression model, the adjusted hazard ratio of asthma patients developing hypothyroidism was calculated. Results: In total, 95,321 asthma patients were selected as the asthma group and the same amount of people without asthma were selected as the control group. The incidence levels of new-onset hypothyroidism in asthma and non-asthma groups were 8.13 and 6.83 per 100,000 people per year, respectively. Compared with the non-asthma group, the adjusted hazard ratio of the asthma group developing hypothyroidism was 1.217 (95% confidence interval, 1.091–1.357). Conclusions: We found having asthma to be associated with an increased risk of hypothyroidism. Clinicians should be concerned regarding the endocrinological and inflammatory interaction between the two diseases while caring for people with asthma.
Altered Plasma Proteins in Myogenous Temporomandibular Disorders
Authors: Ernberg Malin , Jasim Hajer , Wåhlén Karin , Ghafouri Bijar
Published: May 2022

The aims of this study were (1) to compare the levels and interactions of several plasma proteins in patients with myogenous temporomandibular disorders (TMDM) compared to healthy and pain-free controls, (2) to compare the levels and interactions in two TMDM subgroups, myalgia (MYA) and myofascial pain (MFP), and (3) to explore associations between the proteins and clinical data. Thirty-nine patients with TMDM (MFP, n = 25, MYA, n = 14), diagnosed according to the diagnostic criteria for TMD (DC/TMD), aged 38 years, and sex-matched pain-free controls completed an extended DC/TMD Axis II questionnaire and the plasma concentration of 87 biomarkers were analyzed. Nine proteins separated TMDM from controls (p = 0.0174) and 12 proteins separated MYA from MFP (p = 0.019). Pain duration, characteristic pain intensity, pain catastrophizing, perceived stress, and insomnia severity were significantly associated with protein markers (p < 0.001 to p < 0.022). In conclusion, several plasma proteins were upregulated in TMDM and either upregulated or downregulated in MYA compared to MFP. Some proteins in TMDM were associated with pain variables, sleep disturbance, and emotional function. These results show that systemic differences in protein expression exist in patients with TMDM and that altered levels of specific plasma proteins are associated with different clinical variables.
Ultrasound-Guided Femoral Nerve Block in Geriatric Patients with Hip Fracture in the Emergency Department
Authors: Tsai Tou-Yuan , Cheong Kar Mun , Su Yung-Cheng , Shih Ming-Chieh , Chau Su Weng , Chen Mei-Wen , Chen Chien-Ting , Lee Yi-Kung , Sun Jen-Tang , Chen Kuan-Fu , Chen Kuo-Chih , Chou Eric H.
Published: May 2022

Background and Objectives: Systemic analgesics, including opioids, are commonly used for acute pain control in traumatic hip fracture patients in the emergency department (ED). However, their use is associated with high rates of adverse reactions in the geriatric population. As such, the aim of this study was to investigate the impact of lidocaine-based single-shot ultrasound-guided femoral nerve block (USFNB) on the standard care for acute pain management in geriatric patients with traumatic hip fracture in the ED. Methods: This retrospective, single-center, observational study included adult patients aged ≥60 years presenting with acute traumatic hip fracture in the ED between 1 January 2017 and 31 December 2020. The primary outcome measure was the difference in the amount of opioid use, in terms of morphine milligram equivalents (MME), between lidocaine-based single-shot USFNB and standard care groups. The obtained data were evaluated through a time-to-event analysis (time to meaningful pain relief), a time course analysis, and a multivariable analysis. Results: Overall, 607 adult patients (USFNB group, 66; standard care group, 541) were included in the study. The patients in the USFNB group required 80% less MME than those in the standard care group (0.52 ± 1.47 vs. 2.57 ± 2.53, p < 0.001). The multivariable Cox proportional hazards regression models showed that patients who received USFNB achieved meaningful pain relief 2.37-fold faster (hazard ratio (HR) = 2.37, 95% confidence intervals (CI) = 1.73–3.24, p < 0.001). Conclusions: In geriatric patients with hip fractures, a lidocaine-based single-shot USFNB can significantly reduce opioid consumption and provide more rapid and effective pain reduction.
Electrospun PHB/Chitosan Composite Fibrous Membrane and Its Degradation Behaviours in Different pH Conditions
Authors: Zhou Yansheng , Li Ying , Li Daqing , Yin Yidan , Zhou Fenglei
Published: May 2022

Peripheral nerve injury (PNI) is a neurological disorder that causes more than 9 million patients to suffer from dysfunction of moving and sensing. Using biodegradable polymers to fabricate an artificial nerve conduit that replicates the environment of the extracellular matrix and guides neuron regeneration through the damaged sites has been researched for decades and has led to promising but primarily pre-clinical outcomes. However, few peripheral nerve conduits (PNCs) have been constructed from controllable biodegradable polymeric materials that can maintain their structural integrity or completely degrade during and after nerve regeneration respectively. In this work, a novel PNC candidate material was developed via the electrospinning of polyhydroxy butyrate/chitosan (PHB/CS) composite polymers. An SEM characterisation revealed the resultant PHB/CS nanofibres with 0, 1 and 2 wt/v% CS had less and smaller beads than the nanofibres at 3 wt/v% CS. The water contact angle (WCA) measurement demonstrated that the wettability of PHB/CS electrospun fibres was significantly improved by additional CS. Furthermore, both the thermogravimetric analysis (TGA) and differentiation scanning calorimetry (DSC) results showed that PHB/CS polymers can be blended in a single phase with a trifluoracetic solvent in all compositions. Besides, the reduction in the degradation temperature (from 286.9 to 229.9 °C) and crystallinity (from 81.0% to 52.1%) with increasing contents of CS were further proven. Moreover, we found that the degradability of the PHB/CS nanofibres subjected to different pH values rated in the order of acidic > alkaline > phosphate buffer solution (PBS). Based on these findings, it can be concluded that PHB/CS electrospun fibres with variable blending ratios may be used for designing PNCs with controlled biodegradability.
Autologous Bone Marrow Mononuclear Cells (BMMC)-Associated Anti-Inflammatory Nanoparticles for Cardiac Repair after Myocardial Infarction
Authors: Uemura Laercio , Baggio Simeoni Rossana , Bispo Machado Júnior Paulo André , Gavazzoni Blume Gustavo , Kremer Gamba Luize , Sgarbossa Tonial Murilo , Baggio Simeoni Paulo Ricardo , Stadler Tasca Ribeiro Victoria , Silvestre Rodrigo , de Carvalho Katherine Athayde Teixeira , Napimoga Marcelo Henrique , Cesar Francisco Júlio , Guarita-Souza Luiz Cesar
Published: May 2022

To investigate the effect of transplantation of stem cells from the bone marrow mononuclear cells (BMMC) associated with 15d-PGJ2-loaded nanoparticles in a rat model of chronic MI. Chronic myocardial infarction (MI) was induced by the ligation of the left anterior descending artery in 40 male Wistar rats. After surgery, we transplanted bone marrow associated with 15d-PGJ2-loaded nanoparticle by intramyocardial injection (106 cells/per injection) seven days post-MI. Myocardial infarction was confirmed by echocardiography, and histological analyses of infarct morphology, gap junctions, and angiogenesis were obtained. Our results from immunohistochemical analyses demonstrated the presence of angiogenesis identified in the transplanted region and that there was significant expression of connexin-43 gap junctions, showing a more effective electrical and mechanical integration of the host myocardium. This study suggests that the application of nanoparticle technology in the prevention and treatment of MI is an emerging field and can be a strategy for cardiac repair.
Marine Adaptive Sampling Scheme Design for Mobile Platforms under Different Scenarios
Authors: Zhao Yuxin , Zhao Hengde , Liu Yanlong , Deng Xiong
Published: May 2022

Marine adaptive sampling is a technique that makes full use of limited observation resources by selecting the optimal positions. Recently, the design of an adaptive sampling scheme based on a mobile platform has become a research hotspot. However, adaptive sampling system involves multiple subsystems, and the attributes as well as tasks are always different, which may lead to different sampling scenarios. A great deal of research has been conducted for specific situations, especially with fixed starting and ending points. However, systematic design and simulation experiments under various circumstances are still lacking. How to design the adaptive observation system, so as to cope with the observation task under different scenarios, is still a problem worth studying. Aiming to solve this problem, we designed a systematic scheme design process. The process includes setting up and verifying the background field, adopting the hierarchical optimization framework to adapt to different circumstances, and variable adjustments for twin frames. The needs covered in this paper include not having a fixed starting point and ending point, only having a fixed starting point, having a fixed starting point and ending point, increasing sampling coverage, and simple obstacle avoidance. Finally, the relevant conclusions are applied to the multi-platform simultaneous observation scenario. It provides a systematic flow pattern for designing adaptive sampling scheme of mobile platforms.
Numerical Simulation Study on the Layered Phenomenon of Lakes and Reservoirs Destroyed by a Forced Circulation Device
Authors: Xu Jiaxing , Luo Minghan , Ba Dema , Wu Gongde , Xu Ligang , Jeong Taeseop
Published: May 2022

Thermal stratification is a common phenomenon in lakes and reservoirs. It has a significant influence on water quality dynamics. The destruction of the thermal stratification of lakes and reservoirs can affect the water environment, improve the water quality and the water environment quality and prevent the occurrence of eutrophication. In this study, computational fluid dynamics (CFD) combined with a Eulerian two-phase flow model is used to predict the damage caused by an airlift device to the thermal stratification phenomenon of lake water. The results show that the two devices with different sizes can contribute to a certain exchange of kinetic and thermal energy, affecting the liquid velocity and temperature in the lake water under the condition of different gas velocities. Furthermore, the degree of damage to the thermal stratification phenomenon of lake reservoir is small. However, with the same gas velocity, the device with a guide plate can have a greater impact on the liquid velocity and temperature in the lake water. Further prediction results show that the airlift installed with a guide plate can affect the heat transfer of liquid in the lake and reservoir better and destroy the thermal stratification phenomenon effectively. The quantitative results of model prediction can provide an effective basis for future field scale-up experiments.
Evaluation of Dynamic Tensions of Single Point Mooring System under Random Waves with Artificial Neural Network
Authors: Li Peng , Jin Conglin , Ma Gang , Yang Jie , Sun Liping
Published: May 2022

Real-time monitoring of the mooring safety of floating structures is of great significance to their production operations. A deep learning model is proposed here, based on the long short-term memory (LSTM) artificial neural network. Firstly, the numerical simulation is carried out with the single-point mooring system of a Floating Production Storage and Offloading (FPSO) as the training data of LSTM. Then the proposed LSTM is performed. Finally, taking the motion of FPSO which is not encountered by LSTM neural network model as input, we predict the mooring line tension with this model. Here, one FPSO in the South China Sea is taken as a research case, hydrodynamic and mooring models are established, and the network structure and hyper-parameters of the LSTM model are determined. The prediction results of the LSTM under different combinations of wind, wave, and current are compared with the calculation results of AQWA software. The model constructed here can well predict the mooring line tension of different combinations of wind, wave and current.
Passive Seismic Surveys for Beach Thickness Evaluation at Different England (UK) Sites
Authors: Morgan David , Gunn David , Payo Andres , Raines Michael
Published: May 2022

In an era of environmental change leading to rising sea levels and increased storminess, there is a need to quantify the volume of beach sediment on the coast of Britain in order to assess the vulnerability to erosion using cheap, easy-to-deploy and non-invasive methods. Horizontal-to-vertical spectral ratio (HVSR) is a technique that uses the natural background seismic ‘noise’ in order to determine the depth of underlying geological interfaces that have contrasting physical properties. In this study, the HVSR technique was deployed at a number of settings on the coast of England that represented a range of different compositions, geomorphology, and underlying bedrock. We verified the results by comparison to other survey techniques, such as ground-penetrating RADAR, multichannel analysis of surface waves (MASW), and cone penetration tests. At locations where there was sufficient contrast in physical properties of the beach material compared to the underlying bedrock, the beach thickness (and therefore the volume of erodible material) was successfully determined, showing that HVSR is a useful tool to use in these settings.