bluuebunny/crossref_metadata_2025 huggingface dataset:
What makes esports consumers watch streamers' esports live-streaming contents? Extending the theory of planned behavior
Authors: Jang Wooyoung (William) , Choi Wonjun , Kim Min Jung , Song Hyunseok , Byon Kevin K.
Published: January 2024

Purpose

This study aimed to understand better what makes esports fans engage with streamers' live-streaming of esports gameplay. This study used the Theory of Planned Behavior (TPB) and additionally adopted streamer identification and esports game identification as moderating variables.

Design/methodology/approach

Data were collected from streamers' esports content streaming viewers over 18 years of age using an online survey in Amazon M-Turk (N = 307). Based on past esports live-streaming weekly watching hours, which range from 1 to 45 h, the participants were divided into lower (n = 152) and higher (n = 155) frequency groups. PLS-SEM and bootstrapping techniques were used to test the moderated mediation relationships among the constructs.

Findings

This study found a negative moderating effect of past watching experience on the relationship between attitudes and behavioral intention, and it positively moderated the path between perceived behavioral control and behavioral intention. Also, it was found statistically significant direct impacts of streamer identification (STI) and esports game identification (EGI) on attitude and subjective norms. While the indirect impact of STI on behavioral intention through attitude was statistically significant, there were no significant indirect impacts of EGI on attitude and behavioral intention through subjective norms.

Originality/value

Theoretically, this study extends the TPB model by exploring the two identifications (i.e. streamers and esports games) as antecedents of the focal TPB factors (i.e. attitudes, subjective norms and perceived behavioral control) and the moderating effect of prior experience based on high/low weekly watching frequencies. Practically, content creators of esports live-streaming and live-streaming platform managers can use the study’s findings to develop strategies to nurture their current and future viewership.
Corrosion beneath the surface? The emotional navigation of managerialist regulation in German welfare organizations
Authors: Betzelt Sigrid , Bode Ingo , Parschick Sarina
Published: January 2024

Purpose

Regarding how the public regulation of human services is perceived within welfare organizations and how the latter cope with it, the role of emotions (as mediators between structure and agency) proves highly relevant while often being hidden beneath the surface. This article shows how a specific approach to such regulation – here: managerialism – may impact on “emotional regimes” at the organizational and individual level, affecting the attainment of organizational goals and workers' health.

Design/methodology/approach

The article primarily draws on multiple qualitative case studies across two welfare sectors (four organizations) in Germany (continuing education/active inclusion; long-term care). The study research was conducted between 2020 and 2022 and based on 36 interviews with caseworkers and managers, focus group interviews and expert dialogues at industry level.

Findings

The results suggest that the managerialist regulation of welfare services breeds complex and ambiguous emotional regimes. Business-like management techniques elicit various emotions, affecting the motivational basis of human service work. While the experience of hard challenges may raise positive feelings, coping patterns often put strain on organizations and staff alike. In the short run, related emotional regimes tend to make service delivery proceed smoothly, yet in the long run they may have corrosive effects and problematic repercussions on macro-level developments.

Originality/value

The study highlights the role of emotions in publicly regulated human service settings by intermingling macro- and micro-level processes and thereby broadens the perspective of welfare state research as it reveals the impact of (managerialist) regulation on the dynamic organizational chemistry of such settings.
Generativity of enterprise IT infrastructure for digital innovation
Authors: Wang Shan , Mao Ji-Ye , Wang Fang
Published: January 2024

Purpose

Digital innovation requires organizations to reconfigure their information technology infrastructure (ITI) to cultivate creativity and implement fast experimentation. This research inquiries into ITI generativity, an emerging concept demoting a critical ITI capability for organizational digital innovation. More specifically, it conceptualizes ITI generativity across two dimensions—namely, systems and applications infrastructure (SAI) generativity and data analytics infrastructure (DAI) generativity—and examines their respective social and technical antecedents and their impact on digital innovation.

Design/methodology/approach

This research formulates a theoretical model to investigate the social and technical antecedents along with innovation outcomes of ITI generativity. To test this model and its associated hypotheses, a survey was administered to IT professionals possessing knowledge of their organization's IT architecture and digital innovation performance. The dataset, comprising responses from 140 organizations, was analyzed using the partial least squares technique.

Findings

Results reveal that both dimensions of ITI generativity contribute to digital innovation performance, with the effect of DAI generativity being more pronounced. In addition, SAI and DAI generativities are driven by social and technical factors within an organization. More specifically, SAI generativity is positively associated with the usage of a digital application services platform and IT human resources, whereas DAI generativity is positively linked to the usage of a data analytics services platform, data analytics services usability and data analytics human resources.

Originality/value

This research contributes to the literature on digital innovation by introducing ITI generativity as a crucial ITI capability and deciphering its role in digital innovation. It also offers useful insights and guidance for practitioners on how to build ITIs to achieve better digital innovation performance.
The role of regulatory focus in consumers' adoption of virtual fitting rooms (VFRs)
Authors: Lee Hanna , Xu Yingjiao , Porterfield Anne
Published: January 2024

Purpose

Despite the potential of virtual fitting rooms (VFRs) to enhance the consumer experience, their adoption is in the preliminary stages. Little is known about inherent reasons why consumers would adopt VFRs. As consumers' attributional processes can be influenced by their enduring chronic traits, this study aims to investigate the influence of chronic regulatory focus on consumers' VFR adoptions via consumers' perceptions of value provided by VFRs. Additionally, the mediating effects of perceived functional and experiential values were examined. Further, the moderating effect of prior VFR experience was tested to allow for variations in consumer experiences.

Design/methodology/approach

Data were collected via an online survey of 480 consumers who have at least heard of VFRs via convenience sampling. Established measures were utilized to develop the survey questionnaire. Data were analysed using structural equation modelling to test the main model with mediation effects as well as multi-group comparisons to test the moderating effect.

Findings

Empirical results revealed that respective chronic regulatory foci, as preconceived factors that drive consumers' differences in processing, exerted significant influences on consumers' perceptions of VFRs, which, in turn, positively influenced their adoption intention. Also, perceived values mediated the relationship between regulatory foci and consumers' adoption intention. Further, prior VFR experience moderated the relationship between regulatory focus and perceived value.

Originality/value

The paper empirically tested the importance of chronic regulatory foci in understanding consumers' cognitive and affective attributional processes, explaining inherent psychological reasons why consumers would (not) adopt VFRs.
Exploring the psychological benefits of green apparel and its influence on attitude, intention and behavior among Generation Z: a serial multiple mediation study applying the stimulus–organism–response model
Authors: Rūtelionė Aušra , Bhutto Muhammad Yaseen
Published: January 2024

Purpose

This study examines the relationship between green psychological benefits and green apparel purchase behavior using stimulus–organism–response (S-O-R) theory and a serial multiple mediation model.

Design/methodology/approach

Data were collected from 250 Lithuanians using a standardized questionnaire. Convergent and discriminant validity analyses were performed to ensure validity and reliability, and variable relationships were assessed using structural equation modeling.

Findings

The findings revealed utilitarian environmental benefits and warm glow benefits positively impacted Generation Z's (Gen Z's) attitudes toward green apparel. In contrast, the self-expressive benefits had no effect. In addition, attitude and purchase intention significantly predict green apparel purchase behavior. Attitudes and purchase intentions are crucial as serial mediators between green psychological benefits and green apparel purchase behavior.

Originality/value

These findings provide valuable insights for policymakers in Lithuania and highlight the importance of emphasizing the psychological benefits to encourage the purchase of green apparel. Moreover, policymakers and marketers should design apparel products that appeal to Gen Z, promoting green apparel adoption. By leveraging the stimulus–organism–response (S-O-R) theory, this research contributes to understanding pro-environmental behavior. It bridges the gap between attitudes, intentions and actual behavior in sustainable fashion research.
Personal values and travel motivations: the moderating effects of visit experience, gender and age
Authors: Maghrifani Dila , Sneddon Joanne , Liu Fang
Published: January 2024

Purpose

To understand differences in visitors' travel motivations, this study investigates the relations between personal values and travel motivations as well as the moderating effects of visit experience, age and gender on values–motivations relations among Australian visitors visiting Indonesia.

Design/methodology/approach

The multigroup confirmatory factor analysis (MGCFA) was performed using Analysis of Moment Structures (AMOS) to assess the constructs' validity across groups (potential vs repeat visitors; younger vs older visitors; male vs female visitors). A group model comparison thus was run in the multigroup analysis to test whether any differences in values–motivations relationships were significant across the groups.

Findings

This study shows that travel motivations are associated with values in a systematic way, and values–motivations relations can vary by age, gender and visit experience. Specifically, self-enhancement values are associated with escape-seeking motivation and conservation values are associated with assurance-seeking motivations. Whilst, there is no associations found between openness to change values and novelty-seeking motivations and between self-transcendence values and interaction-seeking motivations. Further, values influence travel motivations for potential but not repeat visitors and for younger but not older visitors.

Research limitations/implications

Tailoring marketing strategies to align with visitors' personal values and travel motivations is crucial. Further, acknowledging the moderating influences of visit experience, gender and age in values–motivations relations enables destination marketers to create more effective and targeted approaches for diverse demographic groups in marketing, promotions and destination development.

Originality/value

This study for the first time provides a better explanation on how the travel motivations are formed in relation to values, age, gender and visit experience.
Investigating consumers’ reactions towards female-empowerment advertising (femvertising) and female-stereotypical representations advertising (sex-appeal)
Authors: Negm Eiman
Published: January 2024

Purpose

This study aims to explore the impact of women empowerment and gender-stereotypical advertising exposure on consumers’ attitudes and purchase intents.

Design/methodology/approach

The quantitative research approach was used to assess the women-empowerment and gender-stereotypical advertising on consumers’ attitudes and purchase intentions, explicating the most effective approach in marketing communication in the Arab context, specifically Egypt. Administrated questionnaires were distributed online cross-gender through the use of convenience sampling. The targeted average sample size sought was minimum of 370. Once the data was acquired, partial least square path modelling of structural equation modelling was conducted to test the hypotheses.

Findings

Results show that male consumers and female consumers react to a certain extent differently to women-empowerment and gender-stereotypical advertisings. For instance, gender-stereotypical advertisings effect attitude for both genders; it effects male consumers’ purchase intentions, but not female consumers. Women empowerment advertising is insignificant on brand attitude and purchase intention among male consumers; among female consumers, it has significant impact on brand attitude, but not purchase intention. Nevertheless, both categories of advertising contribute to male consumers’ and female consumers’ perception and attitude towards gender role portrayal.

Originality/value

This study provides noteworthy insights and perspectives on women-empowerment (Femvertising) and gender-stereotypical advertising effects on cross-gender consumers in the Arab region (a region of strong masculine culture values). The emerged conclusions aid as a reference and guide for scholars and practitioners to develop effective advertising that create prospect depictions and build inspired female customer base.
The application of knowledge management in disaster management: past, present and future trends
Authors: Fauzi Muhammad Ashraf , Pradhan Biswajeet , Sapuan Noraina Mazuin , Kusumastuti Ratih Dyah
Published: January 2024

Purpose

The purpose of this study is to review the role of knowledge management (KM) in disaster management and crisis. Disaster causes many detrimental impacts on human lives through loss of life and damage to properties. KM has been shown to dampen the impact of the disaster on the utilization of knowledge among agencies involved and the local communities impacted by disasters.

Design/methodology/approach

Through a bibliometric methodology (co-citation, bibliographic coupling and co-word analysis), this study presents significant themes in the past, current and future predictions on the role of KM in disaster management. In this review paper, 437 publications were retrieved from the Web of Science and analyzed through VOSviewer software to visualize and explore the knowledge map on the subject domain.

Findings

Findings suggest that the significant themes derived are centralized to disaster preparedness during disaster and disaster postrecovery. This review presents a state-of-art bibliometric analysis of the crucial role of KM in building networks and interconnection among relevant players and stakeholders involved in disaster management.

Research limitations/implications

The main implication of this study is how the authorities, stakeholders and local community can integrate the KM system within the three stages of disasters and the crucial role of technologies and social media in facilitating disaster management.

Originality/value

To the best of the authors’ knowledge, this is the first study to present a bibliometric analysis in mapping KM’s past, present and future trends in disaster management.
Stakeholders in strategy-making
Authors: Ackermann Fran , Eden Colin , McKiernan Peter
Published: January 2024

Purpose

Conventional wisdom says stakeholders matter to managers as they develop strategy – but do they? If so, what type of stakeholders matter and what can managers do?

Design/methodology/approach

An in-depth exploration of five deep case studies where senior executives embarked upon strategy development. Analysis revealed five significant factors for managing stakeholders effectively.

Findings

These findings include: determining the nature of a stakeholder, separating those who care about the strategy and its implementation from those who do not but still could impact it; addressing stakeholders at an appropriate level; considering internal as well as external stakeholders and attending to the stakeholders’ responses to proposed strategies and the consequent dynamics created.

Research limitations/implications

(1) The research was conducted with senior managers, and the authors detail the difficulties involved in doing so within the introduction and (2) The research was specific to the healthcare sector, but has relevance to all strategy makers.

Practical implications

This paper explores five factors and their implications and suggests techniques to address them that are well established and available to promote the effective strategic management of stakeholders.

Originality/value

Empirical research in strategy formation with elites is rare because it is difficult to gain access and trust. Empirical research in stakeholder studies is even rarer. By combining the two elements, the authors gather and interpret a unique dataset.
What are the main drivers of private saving in Egypt?
Authors: Emam Hebatalla Atef
Published: January 2024

Purpose

This study aims to investigate the main drivers of private saving in Egypt (2005–2020).

Design/methodology/approach

It employs an autoregressive distributed lag (ARDL) approach for quarterly data on private saving, lagged private saving, real gross domestic product (GDP) growth, public saving, inflation, real interest rate, money supply, current account deficit and unemployment.

Findings

Private saving in Egypt displays persistency and public saving depresses private saving in the short run and long run. Real interest rate, inflation and unemployment have negative and statistically significant impacts on private saving in the short run and long run. The current account deficit displays a negative effect on private saving but is significant only in the short run. Other incorporated variables, like real GDP and money supply, are not statistically significant. This could be attributed to the high consumption rather than saving motive of the Egyptian population and their tendency to rely more on other informal saving channels.

Research limitations/implications

Findings are of policy relevance as unleashing the determinants of private saving guides policymakers in formulating the appropriate sustainable development policies. It also assists in identifying the main obstacles hindering the promotion of private saving and hence major areas for policy intervention, like financial inclusion, poverty eradication, employment generation and structural reforms.

Originality/value

This study contributes to the literature: (1) it tackles private saving figure rather than aggregate saving figure that is covered by similar studies due to lack of consistent data, (2) given the relatively low quality, unavailability and inconsistency of data on private saving in developing countries, investigating the determinants of private saving should be carried out on an individual country basis which is done by this study, (3) this study fulfills the gap in literature related to the lack of up-to-date studies on private saving in Egypt and (4) it relies on quarterly data that could produce more reliable results.
A conceptual framework for host‐associated microbiomes of hybrid organisms
Authors: Camper Benjamin T. , Laughlin Zachary , Malagon Daniel , Denton Robert , Bewick Sharon
Published: January 2024

Hybridization between organisms from evolutionarily distinct lineages can have profound consequences on organismal ecology, with cascading effects on fitness and evolution. Most studies of hybrid organisms have focused on organismal traits, for example, various aspects of morphology and physiology. However, with the recent emergence of holobiont theory, there has been growing interest in understanding how hybridization impacts and is impacted by host‐associated microbiomes. Better understanding of the interplay between host hybridization and host‐associated microbiomes has the potential to provide insight into both the roles of host‐associated microbiomes as dictators of host performance as well as the fundamental rules governing host‐associated microbiome assembly. Unfortunately, there is a current lack of frameworks for understanding the structure of host‐associated microbiomes of hybrid organisms.

In this paper, we develop four conceptual models describing possible relationships between the host‐associated microbiomes of hybrids and their progenitor or ‘parent’ taxa. We then integrate these models into a quantitative ‘4H index’ and present a new R package for calculation, visualization and analysis of this index.

We demonstrate how the 4H index can be used to compare hybrid microbiomes across disparate plant and animal systems. Our analyses of these data sets show variation in the 4H index across systems based on host taxonomy, host site and microbial taxonomic group.

Our four conceptual models, paired with our 4H index and associated visualization tools, facilitate comparison across hybrid systems. This, in turn, allows for systematic exploration of how different aspects of host hybridization impact the host‐associated microbiomes of hybrid organisms.
Population assignment from genotype likelihoods for low‐coverage whole‐genome sequencing data
Authors: DeSaix Matthew G. , Rodriguez Marina D. , Ruegg Kristen C. , Anderson Eric C.
Published: January 2024

Low‐coverage whole‐genome sequencing (WGS) is increasingly used for the study of evolution and ecology in both model and non‐model organisms; however, effective application of low‐coverage WGS data requires the implementation of probabilistic frameworks to account for the uncertainties in genotype likelihoods.

Here, we present a probabilistic framework for using genotype likelihoods for standard population assignment applications. Additionally, we derive the Fisher information for allele frequency from genotype likelihoods and use that to describe a novel metric, the effective sample size, which figures heavily in assignment accuracy. We make these developments available for application through WGSassign, an open‐source software package that is computationally efficient for working with whole‐genome data.

Using simulated and empirical data sets, we demonstrate the behaviour of our assignment method across a range of population structures, sample sizes and read depths. Through these results, we show that WGSassign can provide highly accurate assignment, even for samples with low average read depths (<0.01X) and among weakly differentiated populations.

Our simulation results highlight the importance of equalizing the effective sample sizes among source populations in order to achieve accurate population assignment with low‐coverage WGS data. We further provide study design recommendations for population assignment studies and discuss the broad utility of effective sample size for studies using low‐coverage WGS data.
Meropenem pharmacokinetic/pharmacodynamic target attainment and clinical response in ICU patients: A prospective observational study
Authors: Helset Elin , Cheng Vesa , Sporsem Hilde , Thorstensen Christian , Nordøy Ingvild , Gammelsrud Karianne Wiger , Hanssen Gorm , Ponzi Erica , Lipman Jeffrey , von der Lippe Elisabeth
Published: January 2024

Background

Several studies report lack of meropenem pharmacokinetic/pharmacodynamic (PK/PD) target attainment (TA) and risk of therapeutic failure with intermittent bolus infusions in intensive care unit (ICU) patients. The aim of this study was to describe meropenem TA in an ICU population and the clinical response in the first 72 h after therapy initiation.

Methods

A prospective observational study of ICU patients ≥18 years was conducted from 2014 to 2017. Patients with normal renal clearance (NRC) and augmented renal clearance (ARC) and patients on continuous renal replacement therapy (CRRT) were included. Meropenem was administered as intermittent bolus infusions, mainly at a dose of 1 g q6h. Peak, mid, and trough levels were sampled at 24, 48, and 72 h after therapy initiation. TA was defined as 100% T > 4× MIC or trough concentration above 4× MIC. Meropenem PK was estimated using traditional calculation methods and population pharmacokinetic modeling (P‐metrics®). Clinical response was evaluated by change in C‐reactive protein (CRP), Sequential Organ Failure Assessment (SOFA) score, leukocyte count, and defervescence.

Results

Eighty‐seven patients were included, with a median Simplified Acute Physiology (SAPS) II score 37 and 90 days mortality rate of 32%. Median TA was 100% for all groups except for the ARC group with 45.5%. Median CRP fell from 175 (interquartile range [IQR], 88–257) to 70 (IQR, 30–114) (p < .001) in the total population. A reduction in SOFA score was observed only in the non‐CRRT groups (p < .001).

Conclusion

Intermittent meropenem bolus infusion q6h gives satisfactory TA in an ICU population with variable renal function and CRRT modality, except for ARC patients. No consistent relationship between TA and clinical endpoints were observed.
The effect of a minimum price per unit of alcohol in Scotland on alcohol‐related ambulance call‐outs: A controlled interrupted time−series analysis
Authors: Manca Francesco , Lewsey Jim , Mackay Daniel , Angus Colin , Fitzpatrick David , Fitzgerald Niamh
Published: January 2024

Background and aims

On 1 May 2018, Scotland introduced a minimum unit price (MUP) of £0.50 for alcohol, with one UK unit of alcohol being 10 ml of pure ethanol. This study measured the association between MUP and changes in the volume of alcohol‐related ambulance call‐outs in the overall population and in call‐outs subsets (night‐time call‐outs and subpopulations with higher incidence of alcohol‐related harm).

Design

An interrupted time−series (ITS) was used to measure variations in the daily volume of alcohol‐related call‐outs. We performed uncontrolled ITS on both the intervention and control group and a controlled ITS built on the difference between the two series. Data were from electronic patient clinical records from the Scottish Ambulance Service.

Setting and cases

Alcohol‐related ambulance call‐outs (intervention group) and total ambulance call‐outs for people aged under 13 years (control group) in Scotland, from December 2017 to March 2020.

Measurements

Call‐outs were deemed alcohol‐related if ambulance clinicians indicated that alcohol was a ‘contributing factor’ in the call‐out and/or a validated Scottish Ambulance Service algorithm determined that the call‐out was alcohol‐related.

Findings

No statistically significant association in the volume of call‐outs was found in both the uncontrolled series [step change = 0.062, 95% confidence interval (CI) = −0.012, 0.0135 P = 0.091; slope change = −0.001, 95% CI = −0.001, 0.1 × 10−3 P = 0.139] and controlled series (step change = −0.01, 95% CI = −0.317, 0.298 P = 0.951; slope change = −0.003, 95% CI = −0.008, 0.002 P = 0.257). Similarly, no significant changes were found for the night‐time series or for any population subgroups.

Conclusions

There appears to be no statistically significant association between the introduction of minimum unit pricing for alcohol in Scotland and the volume of alcohol‐related ambulance call‐outs. This was observed overall, across subpopulations and at night‐time.
Australian Foreign Policy Stability and Instability: Imperial Friendships and Crises from the Great Depression to the Fall of Singapore
Authors: Widmaier Wesley W.
Published: January 2024

Over the past century, crises have enabled the construction of Australian foreign policy orders, or sets of ideas that reduce uncertainty and stabilise interests. However, such ideas have also engendered misplaced certainty and renewed crisis. Developing a constructivist framework, I stress the ways in which ideas can over time impede the use of information and fuel instability and crises. In a staged model, I trace the construction of ties with “great and powerful friends”, their conversion in ways that fuel misplaced certainty, and the construction of crises which advance change. Empirically, I then trace the construction of an early Imperial order, misplaced certainty in UK‐backed austerity and appeasement, and crises in the Great Depression and fall of Singapore.
Victim or Threat? Shipwrecks, Terrorist Attacks, and Asylum Decisions in France
Authors: Emeriau Mathilde
Published: January 2024

Tragic events such as terrorist attacks have been shown to influence voters’ policy preferences, but less is known about whether such events also affect actual immigration policy. In this study, I bring new evidence to this question by examining whether migrant shipwrecks and terrorist attacks affected asylum decisions in France during the refugee crisis of 2015–16. I find that asylum officers were more likely to approve an individual's refugee application if a shipwreck has recently been in the news than they are otherwise. Yet they were less likely to grant refugee status to asylum seekers from Syria and Iraq after a terrorist attack. Together, these findings suggest that tragic events can affect immigration policy through their influence on asylum officers.
Does hypometabolism constrain innate immune defense?
Authors: Kadamani Karen L. , Logan Samantha M. , Pamenter Matthew E.
Published: January 2024

Many animals routinely make energetic trade‐offs to adjust to environmental demands and these trade‐offs often have significant implications for survival. For example, environmental hypoxia is commonly experienced by many organisms and is an energetically challenging condition because reduced oxygen availability constrains aerobic energy production, which can be lethal. Many hypoxia‐tolerant species downregulate metabolic demands when oxygen is limited; however, certain physiological functions are obligatory and must be maintained despite the need to conserve energy in hypoxia. Of particular interest is immunity (including both constitutive and induced immune functions) because mounting an immune response is among the most energetically expensive physiological processes but maintaining immune function is critical for survival in most environments. Intriguingly, physiological responses to hypoxia and pathogens share key molecular regulators such as hypoxia‐inducible factor‐1α, through which hypoxia can directly activate an immune response. This raises an interesting question: do hypoxia‐tolerant species mount an immune response during periods of hypoxia‐induced hypometabolism? Unfortunately, surprisingly few studies have examined interactions between immunity and hypometabolism in such species. Therefore, in this review, we consider mechanistic interactions between metabolism and immunity, as well as energetic trade‐offs between these two systems, in hypoxia‐tolerant animals but also in other models of hypometabolism, including neonates and hibernators. Specifically, we explore the hypothesis that such species have blunted immune responses in hypometabolic conditions and/or use alternative immune pathways when in a hypometabolic state. Evidence to date suggests that hypoxia‐tolerant animals do maintain immunity in low oxygen conditions, but that the sensitivity of immune responses may be blunted.
The relationship between populism and attitudes on vaccine against COVID‐19: Trust in institutions as a moderation factor
Authors: Kosic Ankica , Kana Kenfack Corine Stella , Dionisi Eleonora
Published: January 2024

The aim of this study is to examine whether the relationship between populist orientation and attitudes towards the anti‐COVID vaccine and government measures is mediated by conspiracy beliefs and health risk perceptions, and whether these relationships are moderated by trust in institutions. Data were collected in Italy using a questionnaire (N = 390). The results largely supported our hypotheses of moderated mediation. Basically, while previous studies suggest that populist attitudes are positively associated with conspiracy theories, vaccine hesitancy and limited compliance with government measures, this study shows that these relationships are stronger when people do not trust institutions.
Variation among individual beef cattle in methane‐to‐carbon dioxide ratio measured under on‐farm conditions using the sniffer method
Authors: Oikawa Kohei , Kim Minji , Terada Fuminori , Masaki Tatsunori , Yasuda Yaichirou , Shiroshita Yoshikazu , Ideo Takahiro , Kamiya Yuko , Suzuki Tomoyuki
Published: January 2024

This study proposed a method for measuring the methane (CH4)/carbon dioxide (CO2) ratio from individual beef cattle under on‐farm conditions and estimated the variance components of the CH4/CO2 ratio. Gas measurements were conducted using 166 Japanese Black cattle group‐housed in pens equipped with individual feed bins. The gas containing the animal's breath was measured individually after concentrate feeding by covering the feed bin with a sheet with sampling inlets. Measurements were performed six times (three consecutive days, twice daily) per individual. Most of the sampled gas contained more than 1000 ppm of the mean background‐corrected CO2, suggesting that the method proposed in this study successfully collected sufficient breath concentration to accurately measure the CH4/CO2 ratio. The between‐animal variance accounted for 31.7% of the total variance in the CH4/CO2 ratio. The results showed that the gas collection method proposed in this study could be a useful tool for measuring the CH4/CO2 ratio under on‐farm conditions. The variance component obtained from this study will help to establish protocols for generating data for genetic evaluation and performing dietary experiments with sufficient statistical power.
BRCC36 associates with FLT3‐ITD to regulate its protein stability and intracellular signaling in acute myeloid leukemia
Authors: Liu Jianwei , Isaji Tomoya , Komatsu Sachiko , Sun Yuhan , Xu Xing , Fukuda Tomohiko , Fujimura Tsutomu , Takahashi Shinichiro , Gu Jianguo
Published: January 2024

Fms‐like tyrosine kinase‐3 (FLT3) is a commonly mutated gene in acute myeloid leukemia (AML). The two most common mutations are the internal‐tandem duplication domain (ITD) mutation and the tyrosine kinase domain (TKD) mutation. FLT3‐ITD and FLT3‐TKD exhibit distinct protein stability, cellular localization, and intracellular signaling. To understand the underlying mechanisms, we performed proximity labeling with TurboID to identify proteins that regulate FLT3‐ITD or ‐TKD differently. We found that BRCA1/BRCA2‐containing complex subunit 36 (BRCC36), a specific K63‐linked polyubiquitin deubiquitinase, was exclusively associated with ITD, not the wild type of FLT3 and TKD. Knockdown of BRCC36 resulted in decreased signal transducers and activators of transcription 5 phosphorylation and cell proliferation in ITD cells. Consistently, treatment with thiolutin, an inhibitor of BRCC36, specifically suppressed cell proliferation and induced cell apoptosis in ITD cells. Thiolutin efficiently affected leukemia cell lines expressing FLT3‐ITD cell viability and exhibited mutual synergies with quizartinib, a standard clinical medicine for AML. Furthermore, mutation of the lysine at 609 of ITD led to significant suppression of K63 polyubiquitination and decreased its stability, suggesting that K609 is a critical site for K63 ubiquitination specifically recognized by BRCC36. These data indicate that BRCC36 is a specific regulator for FLT3‐ITD, which may shed light on developing a novel therapeutic approach for AML.
SIRT7 promotes Hippo/YAP activation and cancer cell proliferation in hepatocellular carcinoma via suppressing MST1
Authors: Gu Yiying , Ding Cong , Yu Tingzi , Liu Bohao , Tang Wenbin , Wang Zhiqiang , Tang Xiaohui , Liang Gaoshuang , Peng Jinying , Zhang Xiangwen , Li Zhuan
Published: January 2024

Abnormal activation of the oncogene YAP in the Hippo pathway is a major feature in liver cancer and inactivation of MST1/2 has been shown to be responsible for the overactivation of YAP that led to tumorigenesis. However, mechanisms underlying MST1/2 dysregulation remain poorly understood. RNA‐seq analysis and genome (KEGG) pathway enrichment analysis were used to identify genes and pathways that were regulated by SIRT7. qRT‐PCR, ChIP, and luciferase assay were used to investigate transcriptional regulation. Mass spectrometry, co‐immunoprecipitation and immunoprecipitation were used to exam protein–protein interaction and post‐transcriptional modification. A xenograft mouse model was used to confirm the effect of SIRT7 and SIRT7 inhibitors on hepatocellular carcinoma (HCC) proliferation in vivo. We found that SIRT7 suppresses MST1 by both transcriptional regulation and post‐transcriptional modification, which in turn promotes YAP nuclear localization and transcriptional activation in liver cancer. Mechanistically, we revealed that SIRT7 suppresses MST1 transcription by binding to the MST1 promoter and inducing H3K18 deacetylation in its promoter region. In addition, SIRT7 directly binds to and deacetylates MST1, which primes acetylation‐dependent MST1 ubiquitination and protein degradation. In clinical samples, we confirmed a negative correlation between SIRT7 and MST1 protein levels, and high SIRT7 expression correlated with elevated YAP expression and nuclear localization. In addition, SIRT7 specific inhibitor 2800Z sufficiently inhibited HCC growth by disrupting the SIRT7/MST1/YAP axis. Our data thus revealed the previously undescribed function of SIRT7 in regulating the Hippo pathway in HCC and further proved that targeting SIRT7 might provide novel therapeutic options for the treatment of liver cancer.
Choroidal signal hypertransmission on optical coherence tomography imaging: Association with development of geographic atrophy in age‐related macular degeneration
Authors: Wu Zhichao , Terheyden Jan H. , Hodgson Lauren A. B. , Guymer Robyn H.
Published: January 2024

Background

To examine the association between large choroidal signal hypertransmission ≥250 μm (LHyperT) on optical coherence tomography (OCT) with the risk of developing geographic atrophy (GA) and compare this risk with those associated with nascent geographic atrophy (nGA).

Methods

Two hundred and eighty eyes from 140 participants with bilateral large drusen and without late age‐related macular degeneration (AMD) or nGA at baseline underwent OCT imaging and colour fundus photography (CFP) at 6‐monthly intervals up to 5 years. OCT scans were graded for the presence of LHyperT and nGA, and CFPs were graded for the presence of GA.

Results

The five‐year incidence of LHyperT and nGA were 37% and 27% respectively (p = 0.003), and the two‐year probability of their progression to GA were 17% and 40%, respectively (p = 0.002). LHyperT and nGA explained 81% and 91% of the variance in the time to develop GA, respectively (p = 0.032), and they were both associated with a significantly higher rate of GA development compared to eyes without these lesions (adjusted hazard ratio = 110.8 and 183.2, respectively; p < 0.001 for both).

Conclusions

LHyperT and nGA were both high‐risk features for GA development, but the latter showed a higher rate of GA progression and explained a significantly greater proportion of the variance in the time to develop GA. As such, nGA may be a more robust surrogate endpoint than LHyperT for the conventional clinical endpoint of CFP‐defined GA for intervention trials in the early stages of AMD.
Associative learning of visual cues in the fruit fly parasitoid Diachasmimorpha longicaudata: temporal dynamics and potential consequences on females' fitness
Authors: Nussenbaum Ana L. , Devescovi Francisco , Bachmann Guillermo E. , Pacelli Franco , Garbalena Micaela , Segura Diego F.
Published: January 2024

Diachasmimorpha longicaudata (Ashmead) (Hymenoptera: Braconidae) is a generalist larval‐prepupal parasitoid of true fruit flies (Diptera: Tephritidae). During host‐seeking, females use visual information to locate the habitat of the host (fruit of various species). Previous studies have shown that females associate the colour of the host habitat with the availability of hosts through associative learning. In this study, we aimed to understand how this process is reinforced as the number of positive experiences increases and also how stable this association is, when the positive experiences cease. Additionally, under the hypothesis that associative learning has adaptive value, we evaluated the benefits of learning in two contexts: one in which host larvae are still associated with the learned colour and one where larvae are associated with a different colour. Repeated exposure to the unconditional stimulus for 1, 3, 5, and 7 consecutive training bouts influenced the preference response, as the percentage of females that preferred the stimulus increases with the number of training sessions. This preference seems to be reversible, as we found that the memory duration declines as time since the last training bout increased. The results obtained regarding the benefits of learning showed that females that learned a particular colour associated with the host, spent more time in that colour, made more oviposition attempts, and produced more offspring compared to females that learned a colour that is no longer associated with the host. This suggests that learning should not be fixed, and females would benefit from adjusting their behaviour to host availability. Such colour would allow females to find their host more efficiently, by associating cues that lead to host finding but also modifying their response when the cues are no longer associated with a reward, which seems a reasonable strategy for parasitoids of polyphagous hosts infesting ephemeral and changing habitats, such as fruit of different species.
Angular leaf spot of Caucasian alder incited by a pathovar of Xanthomonas arboricola
Authors: Abbasi Valeh , Rahimian Heshmatollah , Tajick‐Ghanbari Mohammad Ali , Barzegar Ali
Published: January 2024

Caucasian alder (Alnus subcordata) is among the major constituents of the Hyrcanian forests extending all along the southern coast of the Caspian Sea in northern Iran. An angular leaf spot with a yellowish chlorotic halo has attained a widespread occurrence throughout the forest and suburban areas of the northern provinces including Guilan, Mazandaran and Golestan. Strains of a Xanthomonas sp. were consistently isolated from the symptomatic leaves of A. subcordata. Colonies of the isolates were yellow circular, convex and mucoid on nutrient agar containing glucose or sucrose. In multilocus sequence analysis using the genes gyrB, rpoD, dnaK, gltA, fyuA and gapA, the representative isolates occupied a clade shared by the known pathovars of Xanthomonas arboricola. The isolates were relatively heterogeneous phenotypically, but more so in their rep‐PCR fingerprints. Pathogenicity of several isolates was confirmed on seedlings of A. subcordata and Alnus rhombifolia, whereas Alnus cordata, Alnus glutinosa and Alnus crispa appeared not to be susceptible. Based on these characteristics, the isolates causing angular leaf spots of Caucasian alder appear to represent a novel pathovar of X. arboricola.
The origins of sedimentation inHusserl's phenomenology
Authors: Geniusas Saulius
Published: January 2024

Husserl is the philosopher who transformed the geological metaphor of sedimentation into a philosophical concept. While tracing the development of Husserl's reflections on sedimentation, I argue that the distinctive feature of Husserl's approach lies in his preoccupation with the question concerning the origins of sedimentations. The paper demonstrates that in different frameworks of analysis, Husserl understood these origins in significantly different ways. In the works concerned with the phenomenology of time consciousness, Husserl searched for the origins of sedimentation in the field of subjective experience, and more precisely, in impressional consciousness. By contrast, in the later works concerned with history, he maintained that the origins of sedimentations lie in the field of historical past that stretches beyond the reach of individual experience. Building on the basis of these resources, I argue that the Husserlian concept of sedimentation has three distinct components of senses: static, genetic, and generative. In the static sense, sedimentations are modifications of retentions and necessary conditions of recollection. In the genetic sense, sedimentations are necessary for the formation of types, habits and moods, and as such, they shape present experiences. In the generative sense, sedimentations refer to what consciousness inherits from the historical tradition.
A systematic review of theories, models and frameworks used for youth engagement in health research
Authors: Sanchez Sherald , Thorburn Rachel , Rea Marika , Kaufman Pamela , Schwartz Robert , Selby Peter , Chaiton Michael
Published: January 2024

Background

Youth engagement in research, wherein youth are involved in the research beyond mere participation as human subjects, is growing and becoming more popular as an approach to research. However, systematic and deliberate theory‐building has been limited. We conducted a systematic review to identify and synthesize theories, models and frameworks that have been applied in the engagement of youth in health research, including mental health.

Methods

Six academic databases (MEDLINE, PsycINFO, Embase, PubMed, Scopus, CINAHL) and the grey literature were searched for relevant studies. Citation tracking was conducted through ancestry and descendancy searches. The final search was completed on 7 February 2023. Findings were summarized in a narrative synthesis informed by principles of hermeneutic analysis and interpretation. Reporting of results is in accordance with the PRISMA (Preferred Reporting Items for Systematic reviews and Meta‐Analyses) 2020 Statement.

Results

Of the 1156 records identified, 16 papers were included, from which we extracted named theories (n = 6), implicit theories (n = 5) and models and frameworks (n = 20) used for youth engagement in health research. We identified theories that were explicitly stated and surfaced theories that were more implicitly suggested. Models and frameworks were organized into four categories based on their principal features: power‐focused (n = 8), process‐focused (n = 7), impact‐focused (n = 3) and equity‐focused (n = 2). Few frameworks (n = 5) were empirically tested in health‐related research.

Conclusions

The state of theoretical development in youth engagement in research is still evolving. In this systematic review, we identified theories, models and frameworks used for youth engagement in health research. Findings from this systematic review offer a range of resources to those who seek to develop and strengthen youth engagement in their own research.

Patient or Public Contribution

Youth engaged as patients in the research were not involved in planning or conducting the systematic review. However, youth researchers in their early to mid‐20s led the planning, implementation and interpretation of the review. As part of subsequent work, we formed a youth advisory board to develop a youth‐led knowledge mobilization intended for an audience of youth with lived experience of being engaged as patients in research.