bluuebunny/crossref_metadata_2025 huggingface dataset:
Hydrogen storage and handling with hydrides
Authors: Baricco Marcello , Dematteis Erika M. , Barale Jussara , Costamagna Mattia , Sgroi Mauro F. , Palumbo Mauro , Rizzi Paola
Published: April 2024

After production and before the use in different applications, hydrogen may need to be purified, transported, compressed and stored. Hydrogen is conventionally stored in high pressure gas cylinders and, as a liquid phase at low temperatures, in opened tanks. These methods present several economic and security problems. So, hydrogen storage in liquid or solid carriers is a suitable method for future applications. Hydrogen absorption and desorption in metal and complex hydrides will be discussed. Examples are provided, including the role of additives in promoting hydrogen sorption reactions. Some case studies using metal hydrides as hydrogen carrier are presented. The HyCARE project, focussed on the development of an efficient metal hydride-based system for the storage of renewables energies is presented, giving evidence of about 50 kg of hydrogen stored in metal hydrides. A small-scale hydrogen refuelling station developed to provide hydrogen for a fuel cell driven drone will be described. The Life Cycle Assessment (LCA) methodology to evaluate the environmental impacts associated with developed systems is also shortly described. Finally, main open challenges will be outlined, suggesting possible approaches for their overcoming.
Computation and analysis of optimal disturbances of stationary solutions of the hepatitis B dynamics model
Authors: Khristichenko Michael Yu. , Nechepurenko Yuri M. , Mironov Ilya V. , Grebennikov Dmitry S. , Bocharov Gennady A.
Published: April 2024

Optimal disturbances of a number of typical stationary solutions of the hepatitis B virus infection dynamics model have been found. Specifically optimal disturbances have been found for stationary solutions corresponding to various forms of the chronic course of the disease, including those corresponding to the regime of low-level virus persistence. The influence of small optimal disturbances of individual groups of variables on the stationary solution is studied. The possibility of transition from stable stationary solutions corresponding to chronic forms of hepatitis B to stable stationary solutions corresponding to the state of functional recovery or a healthy organism using optimal disturbances is studied. Optimal disturbances in this study were constructed on the basis of generalized therapeutic drugs characterized by one-compartment and two-compartment pharmacokinetics.
Constructing low-rank Tucker tensor approximations using generalized completion
Authors: Petrov Sergey
Published: April 2024

The projected gradient method for matrix completion is generalized towards the higher-dimensional case of low-rank Tucker tensors. It is shown that an operation order rearrangement in the common projected gradient approach provides a complexity improvement. An even better algorithm complexity can be obtained by replacing the completion operator by a general operator that satisfies restricted isometry property; however, such a replacement transforms the completion algorithm into an approximation algorithm.
Recurrent gestures and embodied stance-taking in courtroom opening statements
Authors: Yang Min , Wang Min
Published: April 2024

Despite the inherently multimodal nature of the courtroom, studies of multimodality in forensic linguistics have been scarce. This study uses the stance triangle and ideological square concepts and the assumption that recurrent gestures serve as stance-taking resources to analyze conflicting embodied stances taken by the prosecutor and defense attorney in their opening remarks during the State of Minnesota v. Derek Michael Chauvin 2021 trial. Statistical results reveal that the prosecutor predominantly uses modal gestures to display a strongly aggravated oppositional stance and attempts to be more persuasive, while the defense attorney favors performative gestures. The analysis of the interplay between the verbal and gestural resources suggests that recurrent gestures such as open hand prone or supine, open hand held with vertical palms, index finger extended, and precision grip have a threefold indexical realization: prototypical, argumentative, and stance-taking. The findings contribute to a more complete understanding of persuasion in the courtroom.
Reacting flow analysis in scramjet engine: effect of mass flow rate of fuel and flight velocity
Authors: Sanaka Srinivas Prasad , Kandula Ramanaiah , Chalamalasetty Khyathi Sree , Kappala Durga Rao
Published: April 2024

The objective of the study is realizing the effect of fuel mass flow rate and flight speed on combustion in scramjet engine. DLR conical strut based scramjet combustor configuration was chosen and simulated the chemical reaction between the air and hydrogen fuel. A slot of size 40 mm × 0.295 mm provided at the center of the strut to injected hydrogen fuel from the rare side in to the downstream flow. ICEM CFD software is used for the generation of structured elements in computational domain for three dimensional flow analyses. Standard k-epsilon turbulence model and species transport equation is used in ANSYS fluent solver. The predicted temperature, velocity distribution along the axial length was compared with the experimental results and validated. The temperature distribution at different Mach numbers and mass flow rate reveals that the peak temperature increased with the flight speed and inlet fuel mass flow rate. The peak temperature noticed at the center of the combustor is around 3500 K at a flight speed of Mach 4. The predicted variation of temperature, pressure, velocity in the combustor and the flow structure for reacting flow facilitate good understanding of the combustion process in scramjet combustor.
Consistent and effective method to define the mouse estrous cycle stage by a deep learning-based model
Authors: Strauss L , Junnila A , Wärri A , Manti M , Jiang Y , Löyttyniemi E , Stener-Victorin E , Lagerquist M K , Kukoricza K , Heinosalo T , Blom S , Poutanen M
Published: April 2024

The mouse estrous cycle is divided into four stages: proestrus (P), estrus (E), metestrus (M), and diestrus (D). The estrous cycle affects reproductive hormone levels in a wide variety of tissues. Therefore, to obtain reliable results from female mice, it is important to know the estrous cycle stage during sampling. The stage can be analyzed from a vaginal smear under a microscope. However, it is time-consuming, and the results vary between evaluators. Here, we present an accurate and reproducible method for staging the mouse estrous cycle in digital whole-slide images (WSIs) of vaginal smears. We developed a model using a deep convolutional neural network (CNN) in a cloud-based platform, Aiforia Create. The CNN was trained by supervised pixel-level multiclass semantic segmentation of image features from 171 hematoxylin-stained samples. The model was validated by comparing the results obtained by CNN with those of four independent researchers. The validation data included three separate studies comprising altogether 148 slides. The total agreement attested by the Fleiss kappa value between the validators and the CNN was excellent (0.75), and when D, E, and P were analyzed separately, the kappa values were 0.89, 0.79, and 0.74, respectively. The M stage is short and not well defined by the researchers. Thus, identification of the M stage by the CNN was challenging due to the lack of proper ground truth, and the kappa value was 0.26. We conclude that our model is reliable and effective for classifying the estrous cycle stages in female mice.
A Click-Chemistry Approach to New, Potentially Substituted Chemical Structure, Synthesis, And In Vitro Antimicrobial, Cytotoxic, And Antifungal Activity of Novel Chromene Derivatives
Authors: Sankhe Sharad , Kamble Prashant
Published: April 2024

A group of chromene derivatives, labeled as 4a-c, were produced by a three-step process using sodium carbonate as a catalyst. The experiment used a solvent mixture consisting of 96% ethanol and water, with a volume ratio of 1:5. A successful reaction between the corresponding hydroxyl chromenes derivatives and propargyl bromide resulted in the propargyl ether compounds 5a-c, which are derived from chromene-3-carbonitriles. 4H-chromene-chlorophenyl conjugates 7a-c were produced by 1H-1,2,3-triazole-tethered click chemistry with propargyl ethers 5a-c and 1-azido-2-chlorobenzene. Copper ions were the most effective catalysts for these chemical reactions. The yields of 1H-1,2,3-triazole varied between 75% and 80%. The antimicrobial activity of all three triazoles (a-c) was studied in vitro. In the study conducted by MCC in 2010, four compounds were found to be effective against B. subtilis, four against S. aureus, four against P. aeruginosa, and five against E. coli.
Mass Transfer Mechanism Within Commercial PTFE Membranes In Spacer-Filled Direct Contact Membrane Distillation
Authors: Ve Quoc Linh , Do Minh Cuong , Nguyen Thanh Cuong , Nguyen Quoc Huy , Nguyen Quang Lich , Hoang Minh Tuan , Mahmoudi Farzaneh
Published: April 2024

The mass transfer mechanism within commercial PTFE membrane with various nominal pore sizes in spacer-filled direct contact membrane distillation (DCMD) was determined. Mass fluxes and membrane permeability (MP) values for each commercial PTFE membrane were experimentally measured. The MP values increased insignificantly with 2.3% and 4% when the inlet temperature at feed side rose from 400C to 500C in different membrane pore sizes. All investigated mass transfer models except from Dusty Gas model were good enough to simulate the mass transfer inside smaller membrane pore sizes (0.22 µm, and 0.45 µm). Compared to combined diffusion model, the predicted mass fluxes using the overall mass transfer model including the contribution of Poiseuille flow obtained better agreement with experimental results for larger membrane pore size (1 µm). The mean absolute percentage error (MAPE) values for combined diffusion model were up to 16.5% compared with the Ding et al. model or Schofield et al. model (under 3%). Regarding the root mean square error (RMSE), the combined diffusion model obtains larger values than the mass transfer model considering the Poiseuille flow contribution. Consequently, the contribution of Poiseuille flow in mass transfer mechanism within smaller PTFE membrane pores (0.22 µm, and 0.45 µm) could be ignored, however, the contribution of Poiseuille flow to the overall mass transfer should be included in case of larger membrane pores (1 µm), or the case of applied transmembrane hydrostatic pressure.
Synthesis of Novel Nanocomposite CaO/AC/ZnO from Biogenic Wastes of Date Palm Seeds from The Najran Region (Saudi Arabia) and Eggshells for Degradation of Methylene Blue
Authors: ALHEMIARY NABIL A.
Published: April 2024

Metal nanoparticles and activated carbon-based nanocomposite materials are gaining popularity due to their numerous potential applications in a wide range of materials science areas. The current study aims to create novel photocatalyst frameworks for nanocomposites that were synthesized from biogenic waste and used to photodegrade methylene blue (MB) in wastewater under visible light. The activated carbon from date seeds was calcinated at a temperature of 700°C for two hours, and its surface was improved using zinc oxide nanoparticles and calcium oxide nanoparticles manufactured from eggshells at 900oC. XRD, FT-IR, SEM, BET, and TEM analyses were used to characterize the produced nanocomposites. The synthesized nanocomposite photocatalytic activity was evaluated by degrading MB dyes, its model of water pollution. The results showed that the nanocomposite CaO/AC/ZnO has a mesoporous structure with great sorption capability. Also, the photodegradation efficiency of MB using AC, CaO, AC/ZnO, and CaO/AC/ZnO nanocomposite was 33.23, 37.58, 54.61, and 88.92%, respectively, after100 min of sunlight irradiation indicating that photodegradation efficiency increases by improving the surface properties of AC. The CaO/AC/ZnO nanocomposite exhibited a 96.7% removal efficiency of MB following 120 min of visible light radiation exposure. Therefore, the CaO/AC/ZnO nanocomposite has a promising opportunity to function as a versatile and effective photocatalyst for photodegradation of organic dyes in wastewater.
Factors Associated With Prolonged Mental Health Admissions at US Children’s Hospitals
Authors: DePorre Adrienne G. , Hall Matt , Bernstein Alec M. , Nadler Cy , Puls Henry T.
Published: April 2024

BACKGROUND AND OBJECTIVES

Mental health (MH) hospitalizations at medical hospitals are associated with longer length of stay (LOS) compared with non-MH hospitalizations, but patient factors and costs associated with prolonged MH hospitalizations are unknown. The objective of this paper is to assess patient clinical and demographic factors associated with prolonged MH hospitalizations and describe variation in MH LOS across US children’s hospitals.

METHODS

We studied children aged 5 to 20 years hospitalized with a primary MH diagnosis during 2021 and 2022 across 46 children’s hospitals using the Pediatric Health Information System database. Generalized estimating equations, clustered on hospital, tested associations between patient characteristics with prolonged MH hospitalization, defined as those in the 95th percentile or above (>14 days).

RESULTS

Among 42 654 primary MH hospitalizations, most were aged 14 to 18 (62.4%), female (68.5%), and non-Hispanic white (53.8%). The most common primary MH diagnoses were suicide/self-injury (37.4%), depressive disorders (16.6%), and eating disorders (10.9%). The median (interquartile range) LOS was 2 days (1–5), but 2169 (5.1%) experienced a hospitalization >14 days. In adjusted analyses, race and ethnicity, category of MH diagnosis, and increasing medical and MH complexity were associated with prolonged hospitalization.

CONCLUSIONS

Our results emphasize several diagnoses and clinical descriptors for targeted interventions, such as behavioral and inpatient MH resources and discharge planning. Expanded investment in both community and inpatient MH supports have the potential to improve health equity and reduce prolonged MH hospitalizations.
TOWARDS CLICK-THROUGH RATE PREDICTION IN ONLINE ADVERTISING
Authors: Bratus O.S. , Bidyuk P.I.
Published: April 2024

The click-through rate is one of the main indicators based of which decisions are made in online advertising. A generalized approach to forecasting the click-through rate is proposed, taking into account the specifics of the industry data and using appropriate methods of processing categorical data and tree-based classification models. The simulation results can be used in the subsequent stages of analysis of advertising campaigns.
ФУНКЦІОНАЛ КВАЗІПРОТЯЖНОСТІ ТА ЙОГО ЗАСТОСУВАННЯ
Authors: Vovk S.M.
Published: April 2024

Надано означення функціонала квазіпротяжності і запропоновано його застосування для реалізації ефективної обробки даних, спотворених через шум і аномальні значення. Наведено результати чисельного моделювання.
ІНТЕЛЕКТУАЛЬНІ СИСТЕМИ ДЛЯ РОЗВ’ЯЗАННЯ ОБЕРНЕНИХ КОНТАКТНИХ ЗАДАЧ
Authors: Zhushman V.V.
Published: April 2024

Робота присвячена дослідженню застосування інтелектуальних систем для розв’язання обернених контактних задач. Створено експертну систему для розв'язання оберненої контактної задачі взаємодії декількох однозв’язних штампів з пружним півпростором для ідентифікації геометричної форми основ штампів яка була змінена в результаті пошкоджень. Розроблено програмне забезпечення для комп’ютерного моделювання контактної взаємодії, забезпечення комунікації між експертною системою і даними та для реалізації аналізу отриманих результатів.
ЗАСТОСУВАННЯ МЕТОДІВ ПРОЄКЦІЙНО-ІТЕРАЦІЙНОГО ТИПУ ДО РОЗВ’ЯЗАННЯ ІНТЕГРАЛЬНОГО РІВНЯННЯ ФРЕДГОЛЬМА ДРУГОГО РОДУ
Authors: Kiseleva E.M. , Hart L.L.
Published: April 2024

Розглянуті питання застосування комбінованих підходів на основі проєкційних та ітераційних методів – проєкційно-ітеративного і проєкційно-ітераційного – до розв’язання лінійного інтегрального рівняння Фредгольма другого роду. Наведено умови збіжності та оцінки похибки зазначених методів комбінованого типу, алгоритмізовано та програмно реалізовано їх обчислювальні схеми із використанням методу квадратур. Досліджено практичну збіжність та ефективність реалізованих алгоритмів на прикладі розв’язання конкретної задачі.
APPLICATION OF CLUSTERING METHODS FOR DETECTING NETWORK THREATS
Authors: Kiseleva O.М. , Prytomanova O.М. , Filat O.А.
Published: April 2024

The objective of this article is to explore the possibilities of identifying network attacks using cluster analysis methods. Density-based spatial clustering of applications with noise (DBSCAN) and the ordering points to identify the clustering structure (OPTICS) algorithm have been implemented on data representing a simulated network. The findings reveal the most effective algorithmic combinations for distinguishing between regular connections and data associated with network intrusions.
ГРАФІЧНЕ ПЛАНУВАННЯ РОЗВ’ЯЗАННЯ ДОСЛІДНИЦЬКОЇ ПРОБЛЕМИ
Authors: Nakonechna T.V. , Nikulin A.V.
Published: April 2024

Пропонується узагальнений алгоритм і схема графічного планування розв’язання дослідницької проблеми з урахуванням вектору «мета - результат» і аналізом отриманих результатів. Графічна модель об’єкта дослідження (подвійна причинно-наслідкова діаграма) на етапі організації робіт з визначенням факторів і функцій включає діаграму Ісікави. На етапі самих досліджень виконуються операції аналізу і синтезу з отриманням рішень теоретичного і практичного змісту, яким відповідає друга частина (надбудова) подвійної діаграми. Змістовне графічне планування виконано на прикладі розв’язання дослідницької проблеми поздовжньої стійкості прокатки з аналізом отриманих результатів, які були використані при підготовці та захисту ряду кваліфікаційних робіт магістрів і дисертаційних робіт.
Графічне планування розв’язання дослідницької проблеми дозволяє при виконанні робот провести теоретичні та  експериментальні дослідження в стислі календарні терміни при дуже обмеженому фонді ресурсів і часу з досягненням якісних кваліфікаційних оцінок при отриманні другого і третього рівнів вищої освіти.
ЩОДО ОЦІНЮВАННЯ ПАРАМЕТРІВ ОРБІТАЛЬНОГО МАЙЖЕ КРУГОВОГО РУХУ
Authors: Turchyn V.M. , Volkov H.Y. , Pirozhenko A.V.
Published: April 2024

У роботі на простій модельній задачі запропоновані оцінки параметрів форми майже кругової орбіти і досліджені їхні статистичні характеристики.
Virulence properties of nisin-resistant bacteria isolated from raw milk and cheese samples
Authors: Başyiğit Kılıç G. , Yalçın H. , Soyuçok A. , Sudağıdan M.
Published: April 2024

Nisin is a bacteriocin produced by Lactococcus lactis and it is generally recognised as safe by the U.S. Food and Drug Administration. The study aimed to determine the presence of nisin-resistant Staphylococcus, Enterococcus, and Listeria spp. in 157 raw milk and 125 cheese samples. As a result of the isolation procedure, a total of 282 bacterial strains were isolated. 83/282 strains showed resistance to 300 ppm nisin concentration and they were identified by 16S rRNA sequencing. The two most common species were Enterococcus faecalis and Staphylococcus aureus. In both raw milk and cheese samples, nisin-resistant Listeria could not be detected. Generally, enterococci (59/65) showed higher biofilm formation ability than staphylococci (13/18). 38% (25/65) of enterococci and 88% (16/18) of staphylococci were determined to have protease activity in at least one of Skim Milk Agar, Casein Agar, or Milk Agar. In addition, the most common antibiotic resistance in nisin-resistant enterococci was against chloramphenicol. This study revealed that nisin-resistant staphylococci and enterococci are present in raw milk and cheese. In addition, the presence of some virulence factors such as biofilm production, protease activity, and antibiotic resistance in resistant isolates needs to be drawn attention to. Consumption of nisin-resistant microorganisms with virulence factors through food is a food safety risk.
Surface roughness and surface hardness changes in composite resin provisional crown before and after brushing with whitening toothpaste
Authors: Putri Dea S. , Andryas Ika
Published: April 2024

Objective: To determine the surface roughness and surface hardness difference in composite resin provisional crown before and after brushing with whitening toothpaste.

Material and Methods: This was a pre- and post-test group design experimental study. In this study, the sample was a composite resin provisional crown based on bis-acryl. The number of samples in this study was 32 samples, 16 for the surface roughness test and the other 16 for the surface hardness test. Each sample was brushed using a V8-Brushing Machine Modified, then tested for surface roughness and hardness using a Profilometer and Vickers Hardness Tester.

Result: The paired T test was used to analyze the results statistically. There was a significant difference between the groups before and after brushing with whitening toothpaste. (p<0.05).

Conclusion: After simulated brushing with whitening toothpaste, there was a change in the the provisional crown material surface roughness and hardness value.
Knowledge and stigma towards people living with HIV/AIDS: A study among dental hygiene students
Authors: Hidayat Wahyu , Nur’aeny Nanan , Winda Kamilah
Published: April 2024

Objective: This study aims to assess the level of HIV/AIDS-related knowledge and stigma among dental hygiene’ students towards people living with HIV/AIDS. 

Material and Methods: A cross-sectional survey conducted to a senior year dental hygiene student in Politeknik Kesehatan (POLTEKKES) Bandung in November 2019. The sample size determined by a total sampling technique gives rise to 71 participants out of 75 (response rate 94.67%). A self-administered questionnaire was attempted, covered demographic information, participants’ sources of HIV/AIDS knowledge, eleven items concerned to knowledge and five items related to stigma towards PLWHA. 

Results: The average score for the HIV/AIDS knowledge items and the level of stigma were 8.36 and 3.53. Most of the participants considered as having a high knowledge (71.84%). None of them categorized as a low level of knowledge. Our study also found 66.2% of participants willing to treat PLWHA.

Conclusion: The level of HIV/AIDS knowledge considered moderate. However, we still found several mistakes due to the transmission mode of HIV. The stigma’s assessment of PLWHA was low. Nevertheless, unwillingness to live in the same community with PLWHA still indicated.
Accuracy of mesiodistal teeth and dental arch width measurement with conventional plaster and digital model study
Authors: Adindaputri Zenia , Karunia Dyah , Sutantyo Darmawan
Published: April 2024

Objective: This study aims to determine the accuracy of the sum mesiodistal width and the arch width from the conventional plaster model, 2D digital scan, 3D intraoral scanning, and Cone Beam Computed Tomography (CBCT) methods.

Material and Methods: The type of research in this study is a comparative experimental conducted by comparing 5 different sample groups. The study was conducted on 5 study models in each group, the control group (typodont), and the treatment group (the conventional plaster model, 2D digital scan, 3D intraoral scanning, and CBCT). The parameters measured were the sum of mesiodistal width and inter premolar. Each measurement was repeated 3 times by the same observer, then averaged. Statistical analysis using Pearson Correlation to determine the correlation between the accuracy of the measurement method compared to the control group (typodont).

Results: The results showed that there was a significant accuracy correlation (p<0.05) in measuring the sum of mesiodistal width and inter premolar between the control group (typodont) using conventional plaster model measurement methods, 2D digital scan, 3D intraoral scanning, and CBCT.

Conclusion: The conclusion of this study is conventional measurement methods, 2D digital scan, 3D intraoral scanning, and CBCT are accurate in measuring the sum of mesiodistal width and inter premolar width.
The difference in signs of temporomandibular joint disorders between Airforce Pilot Corps, Paskhas Corps, and Supporting Corps in Indonesian Airforce
Authors: Manurung Basaria , Sjamsudin Endang , Hadikrishna Indra
Published: April 2024

Objective: This study aims to analyze the difference in signs of TMJ disorders in Indonesian Air Force soldiers.
Material and Methods: This was a cross sectional study. The research subjects were 39 Indonesian Air Force soldiers who were underwent periodic health check up at Lakespra Dr. Saryanto Jakarta who had met the inclusion and exclusion criteria.. The data obtained were tested with one way Anova, Kruskal Wallis and Mann Whitney test by SPSS.
Results: The three groups had signs of TMJ disorders in different proportions. The Paskhas Corps had the highest percentage of abnormalities in the examination of muscle palpation, TMJ palpation, and TMJ auscultation of 23%, 23%, and 53.85%, respectively. Meanwhile, the Airforce Pilot had the most abnormalities in mandibular movement, about 92%. The results of statistical tests showed that there were significant differences in the TMJ auscultation examination in the three groups, but not on other examinations.
Conclusion: Exercises and heavy physical loads in the Paskhas Corps can affect the masticatory muscles and cause microtrauma in the TMJ resulting in the occurrence of TMJ symptoms is higher.
Correlation between periodontal disease and COVID-19: Systematic review and meta-analysis
Authors: Tenrisau Dhihram , Sudaryo Mondastri K. , Marlina Erni , Nur Syafarudin
Published: April 2024

Objective: The purpose of this systematic review was to overview studies related to the relationship between periodontitis and the severity of COVID-19.
Method: This systematic review and meta-analysis aims to see an overview of studies related to the relationship between periodontal disease and the severity of COVID-19, especially hospitalization. From the systematic review, 12 pieces of literature are included in the eligibility criteria and analyzed (9 reviews and three meta-analyses).
Results: The estimated OR is 2.68 (z test p = 0.006), moderate heterogeneity is 61%. The periodontal treatment for patient COVID-19 were considered to prevent the death and severity of COVID-19.
Conclusion: Periodontal disease had been associated with the severity and mortality of COVID-19, even though the further studies are needed to generalize to other populations. Apart, we hope that COVID-19 patients, get more attention for their periodontal tissue conditions, in order to prevent the death and severity of these patients.
Effectiveness of moringa oleifera leaf extract nanoparticles in cleaning smear layer on the apical third of root canal walls
Authors: Nugroho Juni J. , Jathiasih Musthika , Natsir Nurhayaty
Published: April 2024

Objective: To evaluate the cleanliness smear layer on the apical third of the root canal wall in teeth irrigated with 5% and 10% Moringa leaf extract nanoparticles. Method: Vitro experimental laboratory with posttest control group design. A total of 30 mandibular permanent premolars were decorated to an average tooth length of 13 mm. The teeth were divided into 5 groups, with 6 samples per group and each sample was irrigated with 5 ml for 5 minutes. Group I (negative control) irrigated with aquadest steril, group II (positive control) irrigated with NaOCl 5,25%, group III (positive control) irrigated with a combination of NaOCl 5,25% and EDTA 17%, group IV (test group) irrigated with Moringa leaf extract nanoparticles 5% and group V (test group) irrigated with Moringa leaf extract nanoparticles 10%. All samples were tested for cleanliness of the smear layer on the apical third of the root canal wall using CLSM. Result: Average score cleanliness of the smear layer on the apical third of the root canal wall the highest are groups IV and V but not significantly different from group III. Groups IV and V were significantly higher than groups II and I, while the lowest was group I. Conclusion: Moringa leaf extract nanoparticles 5% and 10% were effective in cleaning the smear layer on the apical third of the root canal wall.
Keywords: Moringa oleifera, nanoparticles, smear layer, Confocal Laser Scanning Microscope
Cytotoxicity differences from roots and leaves ethanol extracts of Annona muricata Linn on HSC-3
Authors: Naftalia M. Rizki  , Moehamad O. Roeslan
Published: April 2024

Objective: The aim of this study is to examine cytotoxicity differences between ethanol extract of A. muricata roots and leaves against HSC-3 cell line.
Material and Methods: HSC-3 cell lines were treated with ethanol extracts of A. muricata roots and leaves with 100, 30, 10, and 1 μg/mL concentrations for 24 hours. The percentage of cell viability was measured using MTT assay.
Results: The results showed significant differences (p <0.001) of treatments group compared to negative control. Ethanol extract of A. muricata roots showed the highest cytotoxicity against HSC-3 cell lines.
Conclusion: There is difference in cytotoxicity between ethanol extracts of A. muricata roots and leaves against HSC-3 cell lines where root extracts showed higher cytotoxicity.
The influence of platelet concentrates with and without metronidazole incorporation pre-centrifuge towards periodontal ligament fibroblast proliferation
Authors: Monika Feby , Syaify Ahmad , Lastianny Sri P.
Published: April 2024

Objective: Horizontal centrifugation was believed may improve platelet, leukocyte, and growth factors in the buffy coat. Previous study of platelet concentrates metronidazole incorporation was able to greatly inhibit bacteria. Metronidazole might help the proliferation with its ability to inhibit pro-inflammatory cytokines. This study intended to examine the influence of C-PRF and i-PRF with and without metronidazole incorporation horizontal pre-centrifugation towards periodontal ligament fibroblast proliferation.
Material and Methods: 2x104 fibroblast cells on the 96 well plates were added with 1 ml of each buffy coat platelet concentrates. The samples were split into five groups: i-PRF, C-PRF, i-PRF metronidazole, C-PRF metronidazole, and control group. Then monitored after 1 day, 3 days, and 5 days using MTT assays. The data were inspected by Two Way ANOVA Test and Post Hoc Test.
Results: The result indicated that cell proliferation of fibroblasts was escalated by the addition of C-PRF, i-PRF metronidazole incorporation, and C-PRF metronidazole incorporation group. While the i-PRF group showed decreasing proliferation cell count.
Conclusion: This study assumed that platelet concentrates (i-PRF and C-PRF) with metronidazole incorporation can increase the cell proliferation higher than i-PRF and C-PRF only.
Prosthetics management of odonto-onycho-dermal dysplasia pediatric patient: A case report
Authors: Nurrahma Rifaat , Syahrir Syakriani , Ikbal Muhammad , Machmud Edy , Irfan Dammar  , Acing H. Mude  , Ike D. Habar  , Ulfa Ludfia , Wahyuni Nurimah , Bazira Ainun
Published: April 2024

Objective:  This case report discussed the treatment pediatric patients with OOOD require extensive prosthetic treatment and required collaboration between professionals.
Methods: A 12-year-old male patient was referred from the pediatric dentistry department with complaints that his of teeth had not eruptingerupted after extraction. Intraoral examination showed that only eruptions occurred exclusively in teeth 55,14,24,25,65,32,34,37,42,43,44,47 eruptions. In this case, intraoral and extraoral examinations, radiographs, impressions of the maxillary and mandibular jaws, and denture makingdenture-making procedures (including such as makingthe fabrication of a regular  denture) were performed.
Results: After 3 months of wearing the removable denture, the maxillary left and right molars were visibly erupted, and the removable denture was then perforated in that area. A month later, the mandibular premolars also erupted, but surgical exposure was performeddone to accelerate the eruption of the teeth.
Conclusion: The delayed eruption of teeth in children due to ectodermal dysplasia is a multifaceted condition. Consequently, for optimal results, interdisciplinary therapy is recommended.
Entering the World Technologically: Early Encounters
Authors: Alfred Nordmann , Daria Bylieva
Published: April 2024

Technology is changing the world and becoming the world itself. The technical work as a world becomes a scheme that accommodates people and things in a seamless manner as they work together in an unforced manner. In generic terms, the relation of works and worlds, and the corresponding experience of a technical world is established with reference to Ludwig Wittgenstein. The practical and metaphorical example is the incubator for premature babies, which creates a safe atmosphere for the life of the newborn. The incubator represents a technically created world that becomes a protective «shell». The early adoption of digital technologies by children is also partly due to the desire of parents for safety and quiet. The success of early introduction to mobile devices relies greatly on new technological devices with touchscreens and visual interfaces. In the digital world, small children have much more power, agency, autonomy, the ability to change the environment, and at the same time have fun. Modern children enter the digital world at almost the same time as the physical world. They cannot distinguish digital and the physical worlds according to a criterion of reality. The convenient world of technology built by adults becomes a shell or even a trap for children that is perceived by them as a natural and necessary part of the world. Children perception becomes magical: they are affected by magic seduction of the touchscreen, which recomposes a reality that is interrupted, suspended, and returns it as modified, technicized.
Ethical Issues in the Field of Assistive Robotics for Elderly People: Philosophical vs Empirical Research
Authors: Elena Seredkina , Armin Grunwald
Published: April 2024

Assistive robotics can provide technological support to meet the growing demand for health and social care as people's physical and cognitive functions decline with age. Companion robots can help elderly people with tasks such as mobility, communication and self-care. But do we have the moral right to delegate the duties of medical personnel to robots? Can robots provide the proper quality of medical care? Such reflections on the future of “machine” healthcare raise serious ethical questions in the field of human-robot interaction and determine not only priority developments in the field of social robotics, but also technological culture as a whole. Contemporary research on ethical issues in the field of assistive robotics for elderly people can be distinguished from two directions - traditional philosophical speculation and applied research with an emphasis on the participation of various stakeholders. The article discusses these two directions in detail, as well as criticism of philosophical and theoretical attitudes from the “empiricists”. In particular, they argue that along with ethical analysis, social expertise and philosophical reasoning, new forms of reflection, dialogue and experimentation are needed that will be much closer to innovative practices and contexts of use. The methodological basis of the article is the principles of Technology Assessment (TA), transdisciplinary research strategies such as the RRI approach, as well as Value-Sensitive Design (VSD). The article describes new options for value-based design, such as Integrative social robotics (ISR), Care-Centered Value-Sensitive Design (CCVSD). These approaches are quite promising for solving philosophical and ethical problems in the field of social robotics for caring for the elderly. But more direct and indirect stakeholders need to be involved in the design and development of medical robots. Based on the Stakeholder Tokens method, it is shown how some ethical problems can be solved in a situation of value conflict between autonomy, human dignity and security.
The Fusion of Human and Machine: David Cronenberg's Perspective on the Problem of the Cyborg.
Authors: Oleg Gurov
Published: April 2024

The article discusses the topic of interaction between technology and human being through the prism of the cyborg concept. The author provides an introduction to this concept, starting with its invention by scientists Manfred Kleins and Nathan Kline in 1960, the subsequent interpretation of the cyborg phenomenon by modern philosophers, and further demonstrates how the problematics surrounding this concept is developed by the film director David Cronenberg in his movies. The relevance of this topic is determined by the fact that technology is currently developing so rapidly and intensively that literally before our eyes the integration of the products of these technologies into social life is beginning to have a serious impact on all its areas, and, according to a number of scientists, is capable of having a qualitative impact on human evolution. In this paper, the author uses the methods of film analysis and interpretation to the greatest extent, as these methods in combination allow us to deal with the movies on various levels in the most productive way. Based on the presented material, the author proves that the concept of the cyborg is a relevant and promising phenomenon that allows us to soberly reflect on the way humans are merging with technology and to predict what consequences this may lead to. For a multifaceted study of this topic, the author also touches upon such phenomena as the transformation of morality and social attitudes in a changing world. The author puts special emphasis on David Cronenberg's most recent film "Crimes of the Future", released in 2022, which, in the opinion of the author of the paper, is a definite manifesto in the career of the filmmaker, where he has collected the most important and significant positions from his previous works.
Zemstvo Doctors of the Osinsky District of the Perm Province in 1870-1919 (to the Discussion about These Specialists Typical Image)
Authors: Dmitry Chernoukhov , Eduard Chernoukhov
Published: April 2024

Already contemporaries, and then several generations of researchers, tried to create a typical image of a zemstvo doctor. The results obtained were very contradictory. According to the supporters of one historiographic tradition, conventionally called “romantic” by us, many zemstvo doctors devoted themselves to selfless service to the common people. Others (conditionally "pragmatic") believe that for these specialists the possibility of self-realization in cities was more attractive. The significant difference of these zemstvo doctors created images is largely determined by quantitative parameters. Supporters of the "romantic" historiographical tradition focus their attention on the life and work of individual specialists who served for a long time in the same local government and/or performed various public functions. Their opponents tend to consider this medical corporation, noting the frequent change of duty station by most of its members, especially in rural areas. For further analysis, painstaking work is needed to create databases for all zemstvo doctors in a wide chronological range. This will make it possible to systematize information about the places and terms of their service, the reasons for their abandonment, previous and subsequent professional activities, changing the self-awareness of this category of the population. The authors created such a database for all 70 zemstvo doctors who served in the Osinsky district of the Perm province in 1870–1919. Its materials testify to the predominantly pragmatic nature of the activities of most of these specialists. The average service life of zemstvo doctors in the Osinsky zemstvo was 3.5 years. Moreover, this indicator was largely ensured by the long-term activity of several physicians. The most common reason for the dismissal of zemstvo doctors in the county was the transition to a service with more comfortable conditions. At the same time, here, as in all other districts of the Perm province, there were some vivid examples of their many years of selfless activity. In general, the creation of a typical image of a zemstvo doctor, even at the level of a separate province or county, seems to us a debatable goal. This big corporation included representatives very different in their life attitudes. Therefore, contemporaries already reasonably selected several typical images of zemstvo doctors at once.
ABCA1 is direct target gene of miR-144-3p in chondrocyte
Authors: Bich Phuong Ho Thi , Dang Quan Nguyen , Kim Tram Phan Thi , Nam Anh Tang Ha , Truc Linh Le Thi
Published: April 2024

MicroRNA144-3p was reported to associate with osteoarthritis (OA) since it was upregulated in this disease. ABCA1 was also found to involve in OA. Bioinformatics algorithms showed ABCA1 was potential targets of miR-144-3p. This study aims to prove ABCA1 is direct targets of miR-144-3p experimentally. Expression of ABCA1 was determined by Realtime RT-PCR after performing the gain- and loss- function of miR-144-3p in chondrocyte. The 3’UTR containing several binding sites of miR-144-3p was subcloning. The vector with binding sites of miR-144-3p mutated was aslo created. Luciferase assay was performed to check the ability of miR-144-3p binding to ABCA1. Realtime RT-PCR showed that the overexpression of miR-144-3p inhibited ABCA1 expression. The Luciferase assay showed that miR-144-3p directly interacted with ABCA1 through its binding sites on the 3’UTR. These data suggested that ABCA1 is the direct target of miR-144-3p.
Wild-type Caenorhabditis sinica, a model nematode for speciation and evolution, massively found in Vietnam
Authors: Tho Son Le , Hong Gam Nguyen Thi , Thi Thu Nguyen , Hong Loan Do Thi
Published: April 2024

Caenorhabditis sinica is a male/female species, in which the genetic diversity is possibly high. Thus, the species is advantageous for the understanding of the mechanisms of diversity, evolution, and adaptation within the nematode genus Caenorhabditis. Previous studies reported the geographic distribution of C. sinica only in China; nonetheless, this should be more convincing for its ecology. We allegedly surveyed the Caenorhabditis species and their diversity in the forests of Vietnam. We found 59 C. sinica strains, and this therefore indicates the vast variation that is 85.88% and 100% identical within isolated wild-type strains. They unequally present in habitats and are enormous in the northern forest, and rarer in the southern forest. The comparison of 18S rDNA barcode sequences from 59 C. sinica isolates by nucleotide sequence alignment showed the consistent diversity among the strains in or off the same ecologies, and all are comparable with the ever-first isolated strain JU727.
Effect of commercial probiotics and antibiotics on the growth of Campylobacter isolated from chicken meat in Ho Chi Minh city markets
Authors: Bao Tran Pham , Anh Thu Nguyen , Hang Tong Thi
Published: April 2024

This study explores the antibiotic susceptibility of Campylobacter, a prominent foodborne pathogen, isolated in Ho Chi Minh city markets and the efficacy of commercial probiotics in inhibiting these bacteria for enhancing food safety and treating Campylobacter infections. Bacteria were isolated from chicken meat in modified coal deoxycholate cefoperazone agar (mCCD), followed by characterization as per standard procedures. Ten isolates with Gram negative, catalase positive and oxidase positive characteristics were collected. Antibiotic susceptibility is ascertained through the determination of the inhibited zone and minimum inhibitory concentration (MIC) of five distinct antibiotics against Campylobacter on Muller Hinton agar plates, culminating in a comprehensive assessment after a 24-hour incubation duration. The antibiotic susceptibility results underscore substantial diversity in Campylobacter isolates among meat samples, thereby accentuating discernible distinctions among the various antibiotic products. The research also evaluated the suitability of 5 commercial probiotic products (re-named as A, B, C, D, and E for fair assessment) by examining their impact on the growth of Campylobacter colonies. The antimicrobial effect of probiotics against Campylobacter is assessed using the agar well diffusion assay and co-culture method. We obtained consistent results from two methods, indicating no variation in Campylobacter species among meat samples but significant variations among probiotic products. The outcomes of this research provide valuable insights into the antimicrobial potential of each probiotic and antibiotic on Campylobacter, informing recommendations for food hygiene practices and underscoring the role of both probiotics and antibiotics in combating Campylobacter infections.
A importância do estágio supervisionado para a formação do estudante da educação profissional e tecnológica
Authors: Castro Mariana Soares , Souza Helton Nonato de
Published: April 2024

O estágio supervisionado é um ato educativo, que constitui o processo de formação dos estudantes da educação profissional técnica de nível médio – EPTNM. Esse trabalho buscou analisar a contribuição do estágio para a formação integral dos indivíduos. Por meio da pesquisa de métodos mistos, foram aplicados questionários aos estudantes e realizadas entrevistas com coordenadores de cursos e servidores da Seção de estágio, amparados por revisão de literatura. Entre os principais resultados encontrados destacam-se o reconhecimento da importância do estágio para a formação cidadã e o interesse da maioria dos estudantes em estudar no IF. Os estudantes buscam ensino de qualidade para dar seguimento no nível superior e demonstram desinteresse pela área técnica.
Política de formação de professores na EBPT
Authors: Zarzicki Danieli Melo , Auler Neiva Maria Frizon
Published: April 2024

Este artigo apresenta os resultados de uma análise realizada com egressos dos Cursos de Licenciatura do Instituto Federal Farroupilha Campus Alegrete-RS das turmas de 2010 à 2014, com a finalidade de identificar o percentual dos que efetivamente atuam na docência[1]. O trabalho investiga como ocorre a oferta de cursos de Licenciatura nos Institutos Federais de Educação, Ciência e Tecnologia, instituições estas voltadas prioritariamente à Educação Profissional, mas que mediante a promulgação da Lei 11.892/2008, surge a obrigatoriedade da destinação de 20% das suas vagas para a formação de professores. Assim, o estudo foi composto, inicialmente, por pesquisa quantitativa na qual efetuou-se um mapeamento dos estudantes concluintes dos cursos de licenciatura do IFFar Alegrete. A partir dos resultados dessa etapa, a pesquisa prosseguiu com enfoque qualitativo, mediante um questionário, com intuito de obter o número de egressos atuantes na docência. Como resultados, verificou-se que nos cursos de licenciatura no Campus Alegrete do período estudado, menos de 20% dos concluintes lecionam dentro da sua área de formação, com mais de 70% dos egressos fora da atividade de docência. [1] Trata-se de parte da pesquisa da dissertação de mestrado realizada no PROFEPT.
A educação profissional no Brasil na perversa confluência de dois projetos políticos
Authors: Mendes Carolina Soares
Published: April 2024

Procura-se perceber como os Institutos Federais de Educação, Ciência e Tecnologia (IFs) foram (re)estruturados e desenvolvem suas atividades dentro da perversa confluência de dois projetos políticos, um democrático-participativo e outro neoliberal (DAGNINO, 2004). Inscritos na colonialidade (QUIJANO, 2020), os IFs contribuem para a organização do trabalho em atendimento às demandas do capital, podendo tender a aprofundamento ou a busca por superação da colonialidade. Busca-se compreender, a partir de marco legal nacional ao longo de três períodos, como os IFs se inscrevem na perversa confluência enquanto projeto de ampliação de acesso à educação profissional e tecnológica (EPT) orientado a concepções de caráter inclusivo, emancipatório e de promoção de cidadania.