bluuebunny/crossref_metadata_2025 huggingface dataset:
Stochastic Processes Shape Bacterial Community Diversity Patterns along Plant Niche Gradients
Authors: Yang Zhiyuan , Xu Jiayi , Li Junlin , He Lirong , Xu Hongwei , Guo Xinrong , Xue Sha , Cao Yang
Published: January 2024

The ecological niche gradient is an important determinant of microbial community structure. In this paper, we studied variation in rhizosphere bacterial diversity and community composition along an ecological niche gradient. We used the high-throughput sequencing of 16S rRNA genes to study changes in the rhizosphere soil microbial communities of six grass and four shrub species during the secondary succession of abandoned farmland on the Loess Plateau of China. A structural equation model (SEM) was employed to disentangle the relative contribution of ecological niche and soil properties to bacterial diversity and community composition. Proteobacteria, Acidobacteria, and Actinobacteria were the dominant phyla of rhizosphere bacteria in all samples. During the dynamics of the plant niche from low to high, bacterial community composition transitioned from Actinobacteria + Acidobacteria to Proteobacteria + Bacteroidetes higher abundance. Moreover, the bacterial diversity and species richness changed with an increasing niche gradient, showing a clear differentiation in the rhizosphere bacterial community of grassland and shrubland. Further, diversity and species richness decreased from the middle niche of B. ischaemum to the poles, indicating that the succession process had not yet reached the climax community stage. Community assembly analysis suggested that the stochastic process gradually strengthened along the increasing ecological niche gradient, especially the drift effect. Furthermore, SEM analysis showed that the ecological niche had significant negative effects on soil properties and bacterial richness, while the effects on bacterial diversity and the stochastic processes of community assembly were weakened and insignificant. Altogether, our findings suggest that the complex interaction of the ecological niche with bacterial diversity and composition was determined by soil properties. Further, bacterial diversity was not necessarily higher with increasing ecological niche gradients.
Fruit Growing: Cultivation Strategies for Sustainable Agriculture and Quality Produce
Authors: Ziogas Vasileios
Published: January 2024

During the last decade, climate change has had a direct impact on several plant-related aspects, such as physiological processes, disease–pest frequency, yield, and the qualitative composition of fruit [...]
Seroprevalence and Risk Factors Associated with Chlamydia abortus Infection in Sheep and Goats in North-Western Italy
Authors: Nogarol Chiara , Marchino Monica , Scala Sonia , Belvedere Manuela , Renna Giovanna , Vitale Nicoletta , Mandola Maria Lucia
Published: January 2024

Chlamydia abortus, although poorly recognized as a human pathogen, is a zoonotic microorganism that can cause many different symptoms in humans, including subclinical infection and fatal illnesses in pregnant women. C. abortus is one of the most common causes of ovine and caprine infectious abortion worldwide, known as the causative agent of the enzootic abortion of ewes (EAE) or ovine enzootic abortion (OEA). To estimate C. abortus seroprevalence and the risk factors related to C. abortus in small ruminants, the sera from 3045 animals (both sheep and goat) belonging to 202 herds were tested and a questionnaire investigating flock management was administered. At the herd level, the true seroprevalence was 56.6% (CI95%: 46.9–66.3%), at sheep-farm and goat-farm level, the true seroprevalence was 71.4% (CI95%: 54.6–88.3%) and 44.8% (CI95%: 41.3–57.0%), respectively. The true seroprevalence was significantly higher among the sheep than the goats. The logistic regression model identified four factors associated with Chlamydia seropositivity: flock size (i.e., farms with >50 heads), contact with cattle, introduction of animals, and Coxiella seropositivity. The study evidenced a high seroprevalence of Chlamydia abortus in small ruminant farms in the Piedmont region. Considering its zoonotic potential and the health consequences in humans, communication to farmers on the importance of vaccination, as well as the sensibilization of farm vets, seem to be strategical.
Prediction of Body Weight by Using PCA-Supported Gradient Boosting and Random Forest Algorithms in Water Buffaloes (Bubalus bubalis) Reared in South-Eastern Mexico
Authors: Gomez-Vazquez Armando , Tırınk Cem , Cruz-Tamayo Alvar Alonzo , Cruz-Hernandez Aldenamar , Camacho-Pérez Enrique , Okuyucu İbrahim Cihangir , Şahin Hasan Alp , Dzib-Cauich Dany Alejandro , Gülboy Ömer , Garcia-Herrera Ricardo Alfonso , Chay-Canul Alfonso J.
Published: January 2024

This study aims to use advanced machine learning techniques supported by Principal Component Analysis (PCA) to estimate body weight (BW) in buffalos raised in southeastern Mexico and compare their performance. The first stage of the current study consists of body measurements and the process of determining the most informative variables using PCA, a dimension reduction method. This process reduces the data size by eliminating the complex structure of the model and provides a faster and more effective learning process. As a second stage, two separate prediction models were developed with Gradient Boosting and Random Forest algorithms, using the principal components obtained from the data set reduced by PCA. The performances of both models were compared using R2, RMSE and MAE metrics, and showed that the Gradient Boosting model achieved a better prediction performance with a higher R2 value and lower error rates than the Random Forest model. In conclusion, PCA-supported modeling applications can provide more reliable results, and the Gradient Boosting algorithm is superior to Random Forest in this context. The current study demonstrates the potential use of machine learning approaches in estimating body weight in water buffalos, and will support sustainable animal husbandry by contributing to decision making processes in the field of animal science.
Joint Stress Analysis of the Navicular Bone of the Horse and Its Implications for Navicular Disease
Authors: Fuss Franz Konstantin
Published: January 2024

The horse’s navicular bone is located inside the hoof between the deep flexor tendon (DDFT) and the middle and end phalanges. The aim of this study was to calculate the stress distribution across the articular surface of the navicular bone and to investigate how morphological variations of the navicular bone affect the joint forces and stress distribution. Joint forces normalised to the DDFT force were calculated from force and moment equilibria from morphological parameters determined on mediolateral radiographs. The stress distribution on the articular surface was determined from the moment equilibrium of the stress vectors around the centre of pressure. The ratio of the proximal to the distal moment arms of the DDFT, as well as the proximo-distal position and extent of the navicular bone, individually or in combination, have a decisive influence on the position and magnitude of the joint force and the stress distribution. If the moment arms are equal and the bone is more proximal, the joint force vector originates from the centre of the joint surface and the joint load is evenly distributed. However, in a more distal position with a longer distal moment arm, the joint force is close to the distal edge, where the joint stress reaches its peak. Degenerative navicular disease, which causes lameness and pathological changes in the distal portion of the bone in sport horses, is likely to be more severe in horses with wedge-shaped navicular bones than in horses with square bones.
The Association between Cesarean Section Delivery and Child Behavior: Is It Mediated by Maternal Post-Traumatic Stress Disorder and Maternal Postpartum Depression?
Authors: Grisbrook Marie-Andrée , Dewey Deborah , Cuthbert Colleen , McDonald Sheila , Ntanda Henry , Letourneau Nicole
Published: January 2024

Cesarean sections (C-sections) account for up to 21% of births worldwide. Studies have linked delivery via C-section with an increased risk of child behavior problems, such as internalizing and externalizing behaviors. Maternal postpartum depression (PPD) is also linked to child behavioral problems and may play a mediating role in the association between the mode of delivery and child behavior. Mixed findings between mode of delivery and PPD may be due to a failure to distinguish between C-section types, as unplanned/emergency C-sections are linked to post-traumatic stress disorder (PTSD), which has been linked to PPD. The objectives of this study were to determine whether, (1) compared with spontaneous vaginal delivery (SVD) and planned C-section, unplanned/emergency C-sections are associated with increased child behavior problems at two to three years of age and (2) maternal PTSD and PPD mediate the association between delivery type and child behavior problems. A secondary data analysis was conducted on 938 mother–child dyads enrolled in the Alberta Pregnancy Outcomes and Nutrition (APrON) study. Conditional process modeling was employed. Child behavior was assessed using the Child Behavior Checklist (CBCL) 1.5–5 years, and maternal PPD and PTSD were assessed using the Edinburgh Postnatal Depression Scale (EPDS) and the Psychiatric Diagnostic Screening Questionnaire (PDSQ), respectively. No associations were found between delivery type and child behaviors; however, the indirect effect of emergency C-section on child behaviors was significant via the mediating pathway of maternal PTSD on PPD symptoms.
Office Design Features and Future Organizational Change toward Supporting Sustainability
Authors: Binyaseen Adel M. A.
Published: January 2024

Organizational change, influenced by such factors as economics, information and communications technologies (ICTs), and users’ wellbeing, is essential for organizational effectiveness, productivity, and sustainability. Thus, there has been a call for urgent changes in workplace design features to respond to future change. This study is concerned with defining the level of change in design features that is needed in existing Saudi governmental workplaces to cope with future visions of sustainability, as well as controlling the cost of such redesigning. It is hypothesized that future challenges related to economics, ICTs, and users’ wellbeing would have a significant impact on the need to change existing workplace design features. Administrator workplaces in three buildings of Saudi organizations and their representative economic, ICT, and wellbeing trends were considered (n = 104, 95, 96). The average level of change in design features in each building was observed and reported. The most changed design features in buildings affected by economic, ICT, and wellbeing trends were minimizing of luxury workplaces, minimizing storage space, and improving thermal/lighting conditions, respectively. However, the most changed design features that were common to all three buildings were spatial and furniture layouts and minimizing storage space.
Rotational Stiffness Investigation and Parametric Analysis of a Novel Assembled Joint in Lattice Shells
Authors: Xu Jianshe , Zhu Yazhi , Wu Jin , Lu Jin , Zhang Qian , Wang Wei
Published: January 2024

Although there are currently many types of lattice shell joints with different characteristics, assessing the flexural capacity of lattice shell joints is always a great challenge. In this paper, a fan-shaped assembled joint and a welded joint for comparison were subjected to bending tests to investigate the flexural behavior and rotational stiffness of the assembled joint. The strain distribution, load–displacement curve, moment–rotation curve, and damage modes of key parts were analyzed to determine the vulnerable parts of the joints. Our test results show that, with an initial rotational stiffness of about one third of that of the welded joint, the assembled joint specimen exhibits the obvious characteristics of a semi-rigid joint. The finite element analysis results were in good agreement with the experimental results. The results of our parametric analysis show that the rotational stiffness and ultimate moment of the assembled joint increase with increases in the spacing of the bolts and the number of bolts. The performance of the high-strength bolts had a significant influence on the flexural stiffness of the assembled joints. The spacing of the bolts and the number of bolts for the assembled joint are suggested to be greater than the height of the member section and more than three, respectively. The proposed theoretical formula can approximately simulate the initial rotational stiffness of the joint. More in-depth investigations are required in the future for assessing the mechanical behavior of FSA joints subjected to combined bending–compression loads.
Trends in the Incidence of Bronchopulmonary Dysplasia after the Introduction of Neurally Adjusted Ventilatory Assist (NAVA)
Authors: Mehra Kashish , Kresch Mitchell
Published: January 2024

Objective: This study investigates the difference in the rates of bronchopulmonary dysplasia in very low birth weight infants before and after the introduction of neurally adjusted ventilatory assist (NAVA). Study Design: A retrospective cohort study comparing rates of Bronchopulmonary dysplasia (BPD) before and after implementation of NAVA. Eligibility criteria included all very low birth weight VLBW neonates needing ventilation. For analysis, each cohort was divided into three subgroups based on gestational age. Changes in the rate of BPD, length of stay, tracheostomy rates, invasive ventilator days, and home oxygen therapy were compared. Results: There were no differences in the incidence of BPD in neonates at 23–25 6/7 weeks’ and 29–32 weeks’ gestation between the two cohorts. A higher incidence of BPD was seen in the 26–28 5/7 weeks’ gestation NAVA subgroup compared to controls (86% vs. 68%, p = 0.05). No significant difference was found for ventilator days, but infants in the 26–28 6/7 subgroup in the NAVA cohort had a longer length of stay (98 ± 34 days vs. 82 ± 24 days, p = 0.02), a higher percentage discharged on home oxygen therapy (45% vs. 18%, respectively, p = 0.006), and higher tracheostomy rates (3/36 vs. 0/60, p = 0.02), compared to the control group. Conclusions: The NAVA mode was not associated with a reduction in BPD when compared to other modes of ventilation. Unexpected increases were seen in BPD rates, home oxygen therapy rates, tracheostomy rates, and the length of stay in the NAVA subgroup born at 26–28 6/7 weeks’ gestation.
Does Massive Bowel Resection in Newborns Affect Further Immunity in Children?
Authors: Sznurkowska Katarzyna , Borkowska Anna , Zagierska Agnieszka , Malanowska Magdalena , Zieliński Maciej , Zagierski Maciej , Trzonkowski Piotr , Łosin Marcin , Szlagatys-Sidorkiewicz Agnieszka
Published: January 2024

Background: The massive resection of the small intestine leading to short bowel syndrome (SBS) deprives an organism of many immunocompetent cells concentrated in gut-associated lymphoid tissue, the largest immune organ in humans. We have aimed to access the influence of bowel resection on adaptive immunity in children, based on peripheral lymphocyte subsets and serum immunoglobulins. Methods: 15 children who underwent bowel resection in the first months of their life and required further home parenteral nutrition were enrolled into the study. Based on flow cytometry, the following subsets of lymphocytes were evaluated: T, B, NK, CD4+, C8+, and activated T cells. Results: Statistically significant differences were found for the rates of lymphocytes B, T, CD8+, and NK cells. The absolute count of NK cells was lower in the SBS group than in the control group. Absolute counts of lymphocytes, lymphocytes B, T, CD4+, and percentages of lymphocytes CD4+, and activated T cells inversely correlated with age in SBS group. Conclusions: Children with SBS do not present with clinical signs of immunodeficiency as well as deficits in peripheral lymphocyte subsets and serum immunoglobulins. The tendency of the lymphocyte subpopulations to decrease over time points out the necessity for longer follow- up.
SEM-Guided Finite Element Simulation of Thermal Stresses in Multilayered Suspension Plasma-Sprayed TBCs
Authors: Amer Mohamed , Abdelgawad Ahmed , Curry Nicholas , Arshad Muhammad , Hayat Qamar , Janik Vit , Nottingham Jon , Bai Mingwen
Published: January 2024

This study presents novel insights into thermal stress development and crack propagation mechanisms in single- and multilayered suspension plasma-sprayed (SPS) coatings of gadolinium zirconate (GZ) and yttria-stabilized zirconia (YSZ), thermally treated at 1150 °C. By combining image processing with finite element simulation, we pinpointed sites of high-stress concentration in the coatings, leading to specific cracking patterns. Our findings reveal a dynamic shift in the location of stress concentration from intercolumnar gaps to pores near the top coat/thermally grown oxide (TGO) interface with TGO thickening at elevated temperatures, promoting horizontal crack development across the ceramic layers. Significantly, the interface between the ceramic layer and TGO was found to be a critical area, experiencing the highest levels of both normal and shear stresses. These stresses influence failure modes: in double-layer SPS structures, relatively higher shear stresses can result in mode II failure, while in single-layer systems, the predominant normal stresses tend to cause mode I failure. Understanding stress behavior and failure mechanisms is essential for enhancing the durability of thermal barrier coatings (TBCs) in high-temperature applications. Therefore, by controlling the interfaces’ roughness along with improving interfacial toughness, the initiation and propagation of cracks can be delayed along these interfaces. Moreover, efforts to optimize the level of microstructural discontinuities, such as intercolumnar gaps and pores, within the creaming layer and close to the TGO interface should be undertaken to reduce crack formation in the TBC system.
Interactive Homework: A Tool for Parent Engagement
Authors: Moore Laura , Ronau Robert N.
Published: January 2024

Families have largely been excluded from the implementation of the Common Core State Standards in Mathematics (CCSSM), reducing their ability to extend their child’s mathematics learning. CCSSM emphasizes different instructional elements (e.g., pictorial representations, problem solving, multiple strategies for solving) that may differ greatly from how parents learned mathematics. In addition, many school officials have ineffectively engaged parents in the changes, further diminishing their capacity to participate in their child’s learning. This case study examined parent mathematics self-efficacy and parent mathematics knowledge for teaching, factors that influence the effectiveness of their engagement in their child’s mathematics learning. This study was also implemented to identify elements that the parent participant found helpful for their child’s mathematics learning. A thematic analysis was performed on the data sources, the interactive homework assignments, a survey, observations, a researcher’s journal, and an interview to conclude that the interactive homework assignments improved parent mathematics self-efficacy and parent mathematics knowledge for teaching. The parent participant also identified the assignments’ side-by-side examples, additional practice, and the easy access of the assignments as features of the intervention that enhanced her ability to support her child.
Optimizing China’s Afforestation Strategy: Biophysical Impacts of Afforestation with Five Locally Adapted Forest Types
Authors: Ma Wei , Wang Yue
Published: January 2024

Recent research has mapped potential afforestation land to support China’s goal of achieving “carbon neutrality” and has proposed tree species selection to maximize carbon uptake. However, it overlooked biophysical climatic effects, which have a more significant impact on local temperature than CO2 reduction. This study aims to present a comprehensive understanding of how afforestation in China affects local and regional climates through biophysical processes. It focuses on the latitudinal patterns of land surface temperature differences (ΔLST) between five locally adapted forest types and adjacent grasslands using satellite-based observations. Our key findings are as follows: Firstly, broadleaf forests and mixed forests exhibit a stronger cooling effect than coniferous forests due to differences in canopy structure and distribution. Specifically, the net cooling effects of evergreen broadleaf forests (EBFs), deciduous broadleaf forests (DBFs), and mixed forests (MFs) compared to grasslands are −0.50 ± 0.10 °C (mean ± 95% confidence interval), −0.33 ± 0.05 °C, and −0.36 ± 0.06 °C, respectively, while evergreen needleleaf forests (ENFs) compared to grasslands are −0.22 ± 0.11 °C. Deciduous needleleaf forests (DNFs) exhibit warming effects, with a value of 0.69 ± 0.24 °C. In regions suitable for diverse forest types planting, the selection of broadleaf and mixed forests is advisable due to their enhanced local cooling impact. Secondly, temperate forests have a net cooling effect to the south of 43° N, but they have a net warming effect to the north of 48° N compared to grasslands. We recommend caution when planting DNFs, DBFs, and MFs in northeastern China, due to the potential for local warming. Thirdly, in the mountainous areas of southwestern China, especially when planting ENFs and MFs, tree planting may lead to local warming. Overall, our study provides valuable supplementary insights to China’s existing afforestation roadmap, offering policy support for the country’s climate adaptation and mitigation efforts.
Single Nucleotide Variants (SNVs) of the Mesocorticolimbic System Associated with Cardiovascular Diseases and Type 2 Diabetes: A Systematic Review
Authors: Merzah Mohammed , Natae Shewaye , Sándor János , Fiatal Szilvia
Published: January 2024

The mesocorticolimbic (MCL) system is crucial in developing risky health behaviors which lead to cardiovascular diseases (CVDs) and type 2 diabetes (T2D). Although there is some knowledge of the MCL system genes linked to CVDs and T2D, a comprehensive list is lacking, underscoring the significance of this review. This systematic review followed PRISMA guidelines and the Cochrane Handbook for Systematic Reviews of Interventions. The PubMed and Web of Science databases were searched intensively for articles related to the MCL system, single nucleotide variants (SNVs, formerly single nucleotide polymorphisms, SNPs), CVDs, T2D, and associated risk factors. Included studies had to involve a genotype with at least one MCL system gene (with an identified SNV) for all participants and the analysis of its link to CVDs, T2D, or associated risk factors. The quality assessment of the included studies was performed using the Q-Genie tool. The VEP and DAVID tools were used to annotate and interpret genetic variants and identify enriched pathways and gene ontology terms associated with the gene list. The review identified 77 articles that met the inclusion criteria. These articles provided information on 174 SNVs related to the MCL system that were linked to CVDs, T2D, or associated risk factors. The COMT gene was found to be significantly related to hypertension, dyslipidemia, insulin resistance, obesity, and drug abuse, with rs4680 being the most commonly reported variant. This systematic review found a strong association between the MCL system and the risk of developing CVDs and T2D, suggesting that identifying genetic variations related to this system could help with disease prevention and treatment strategies.
Council of Europe Resolution on the Implementation of Pharmaceutical Care—A Step Forward in Enhancing the Appropriate Use of Medicines and Patient-Centred Care
Authors: Henman Martin C. , Ravera Silvia , Lery Francois-Xavier
Published: January 2024

Pharmaceutical care was proposed to address morbidity and mortality associated with medicine-related problems. It utilises the pharmacist’s expertise in medicines, their relationship with the patient and cooperation with other healthcare professionals to optimise the use of medicines. The European Directorate for the Quality of Medicines & HealthCare (EDQM), part of the Council of Europe, found significant variation in the acceptance of pharmaceutical care and in the implementation of pharmaceutical care in Europe. A multidisciplinary group was established to draft a statement of principles and recommendations concerning pharmaceutical care. Through face-to-face meetings, circulation of draft texts and informal consultation with stakeholders, the group produced a resolution. On 11 March 2020, the resolution was adopted by the Committee of Ministers of the Council of Europe. It explains pharmaceutical care and illustrates pharmacists’ contribution to medicine optimisation in different care settings. Pharmaceutical care’s value to health services and its place in health policy were emphasised by addressing the risks and harms from suboptimal use of medicines. Pharmaceutical care can improve medicine use, promote rational use of healthcare resources and reduce inequalities in healthcare by realigning the roles and responsibilities of pharmacists and healthcare professionals. EDQM will promote and advocate for the implementation of pharmaceutical care by enacting practice Resolution CM/Res(2020)3.
Multifractal Heart Rate Value Analysis: A Novel Approach for Diabetic Neuropathy Diagnosis
Authors: Coppola Andrea , Conte Sergio , Pastore Donatella , Chiereghin Francesca , Donadel Giulia
Published: January 2024

Type 2 diabetes mellitus (T2DM) is characterized by several complications, such as retinopathy, renal failure, cardiovascular disease, and diabetic neuropathy. Among these, neuropathy is the most severe complication, due to the challenging nature of its early detection. The linear Hearth Rate Variability (HRV) analysis is the most common diagnosis technique for diabetic neuropathy, and it is characterized by the determination of the sympathetic–parasympathetic balance on the peripheral nerves through a linear analysis of the tachogram obtained using photoplethysmography. We aimed to perform a multifractal analysis to identify autonomic neuropathy, which was not yet manifest and not detectable with the linear HRV analysis. We enrolled 10 healthy controls, 10 T2DM-diagnosed patients with not-full-blown neuropathy, and 10 T2DM diagnosed patients with full-blown neuropathy. The tachograms for the HRV analysis were obtained using finger photoplethysmography and a linear and/or multifractal analysis was performed. Our preliminary results showed that the linear analysis could effectively differentiate between healthy patients and T2DM patients with full-blown neuropathy; nevertheless, no differences were revealed comparing the full-blown to not-full-blown neuropathic diabetic patients. Conversely, the multifractal HRV analysis was effective for discriminating between full-blown and not-full-blown neuropathic T2DM patients. The multifractal analysis can represent a powerful strategy to determine neuropathic onset, even without clinical diagnostic evidence.
Bank Market Power, Firm Performance, Financing Costs and Capital Structure
Authors: Gonçalves Marisa Pessoa , Reis Pedro M. Nogueira , Pinto António Pedro
Published: January 2024

In this study, we provide a thorough analysis, conducted on a company-by-company basis, of the impact of bank concentration and the bank-relative power of banks on firm profitability, financing costs, and capital structure in a small economy like Portugal. Using a sample of 434,990 Portuguese companies, the study spans a time frame of 13 years (from 2006 to 2018). Principal component analysis (PCA) was used to determine bank concentration, and a new variable, “bank-related power”, was introduced. This work employed linear regression with static panel data for fixed and pooled effects, using Driscoll–Kraay standard errors and robust standard error estimation. A direct association was found between business performance and the use of bank credit in highly concentrated banking markets (SMEs), and there is evidence of an inverse relationship when the relative power of banks increases (small business). Evidence also shows that financing costs increase with greater bank concentration, while firms’ capital structure improves under similar conditions. When a bank holds greater relative market power, it tends to exert a negative impact on the capital structure of large companies. However, an inverse relationship is observed in the case of SMEs. Unlike previous studies, the article assesses the effects of bank market power on each of the different companies involved by using both bank concentration (as a composite variable) and a new variable that measures the relative power of banks. Due to its extensive database and expanded time frame, this research is innovative in the context of small-sized companies.
Australian Public Perspectives on Genomic Newborn Screening: Risks, Benefits, and Preferences for Implementation
Authors: Lynch Fiona , Best Stephanie , Gaff Clara , Downie Lilian , Archibald Alison D. , Gyngell Christopher , Goranitis Ilias , Peters Riccarda , Savulescu Julian , Lunke Sebastian , Stark Zornitza , Vears Danya F.
Published: January 2024

Recent dramatic reductions in the timeframe in which genomic sequencing can deliver results means its application in time-sensitive screening programs such as newborn screening (NBS) is becoming a reality. As genomic NBS (gNBS) programs are developed around the world, there is an increasing need to address the ethical and social issues that such initiatives raise. This study therefore aimed to explore the Australian public’s perspectives and values regarding key gNBS characteristics and preferences for service delivery. We recruited English-speaking members of the Australian public over 18 years of age via social media; 75 people aged 23–72 participated in 1 of 15 focus groups. Participants were generally supportive of introducing genomic sequencing into newborn screening, with several stating that the adoption of such revolutionary and beneficial technology was a moral obligation. Participants consistently highlighted receiving an early diagnosis as the leading benefit, which was frequently linked to the potential for early treatment and intervention, or access to other forms of assistance, such as peer support. Informing parents about the test during pregnancy was considered important. This study provides insights into the Australian public’s views and preferences to inform the delivery of a gNBS program in the Australian context.
The Lifespan of D. melanogaster Depends on the Function of the Gagr Gene, a Domesticated gag Gene of Drosophila LTR Retrotransposons
Authors: Balakireva Yevgenia , Nikitina Maria , Makhnovskii Pavel , Kukushkina Inna , Kuzmin Ilya , Kim Alexander , Nefedova Lidia
Published: January 2024

(1) Background: The Gagr gene in Drosophila melanogaster’s genome originated from the molecular domestication of retrotransposons and retroviruses’ gag gene. In all Drosophila species, the Gagr protein homologs exhibit a conserved structure, indicative of a vital role. Previous studies have suggested a potential link between the Gagr gene function and stress responses. (2) Methods: We compared flies with Gagr gene knockdown in all tissues to control flies in physiological tests and RNA-sequencing experiments. (3) Results: Flies with the Gagr gene knockdown exhibited shorter lifespans compared to control flies. Transcriptome analysis revealed that Gagr knockdown flies showed elevated transcription levels of immune response genes. We used ammonium persulfate, a potent stress inducer, to elicit a stress response. In control flies, ammonium persulfate activated the Toll, JAK/STAT, and JNK/MAPK signaling pathways. In contrast, flies with the Gagr gene knockdown displayed reduced expression of stress response genes. Gene ontology enrichment analysis identified categories of genes upregulated under ammonium persulfate stress in control flies but not in Gagr knockdown flies. These genes are involved in developmental control, morphogenesis, and central nervous system function. (4) Conclusion: Our findings indicate the significance of the Gagr gene in maintaining immune response and homeostasis.
Technological Oceanography
Authors: Emelianov Mikhail , Shapiro Georgy I.
Published: January 2024

Advances in our understanding of phenomena in the ocean would not be possible without innovation [...]
Three New Species of Apiospora (Amphisphaeriales, Apiosporaceae) on Indocalamus longiauritus, Adinandra glischroloma and Machilus nanmu from Hainan and Fujian, China
Authors: Liu Xinye , Zhang Zhaoxue , Wang Shi , Zhang Xiuguo
Published: January 2024

Apiospora is widely distributed throughout the world, and most of its hosts are Poaceae. In this study, Arthrinium-like strains were isolated from non-Poaceae in the Hainan and Fujian provinces of China. Based on the combined DNA sequence data of the internal transcriptional spacer (ITS), partial large subunit nuclear rDNA (LSU), translation extension factor 1-α gene (TEF1-α) and β-tubulin (TUB2), the collected Apiospora specimens were compared with known species, and three new species were identified. Based on morphological and molecular phylogenetic analyses, Apiospora adinandrae sp. nov., A. bawanglingensis sp. nov. and A. machili sp. nov. are described and illustrated.
Numerical Identification of Boundary Conditions for Richards’ Equation
Authors: Koleva Miglena N. , Vulkov Lubin G.
Published: January 2024

A time stepping quasilinearization approach to the mixed (or coupled) form of one and two dimensional Richards’ equations is developed. For numerical solution of the linear ordinary differential equation (ODE) for 1D case and elliptic for 2D case, obtained after this semidiscretization, a finite volume method is used for direct problems arising on each time level. Next, we propose a version of the decomposition method for the numerical solution of the inverse ODE and 2D elliptic boundary problems. Computational results for some soil types and its related parameters reported in the literature are presented.
Lactiplantibacillus plantarum Induces Apoptosis in Melanoma and Breast Cancer Cells
Authors: Budu Oana , Mioc Alexandra , Soica Codruta , Caruntu Florina , Milan Andreea , Oprean Camelia , Lighezan Daniel , Rotunjanu Slavita , Ivan Viviana , Banciu Christian
Published: January 2024

Despite the notable advancements witnessed in the past decade in medical and health research domain, cancer remains a prominent global cause of mortality. Moreover, the conventional treatments employed to combat this disease have been found to considerably compromise the quality of life experienced by patients due to its severe side effects. Recent in vitro studies revealed encouraging findings on the potential beneficial effects of probiotics as adjuvants of anticancer therapy, and even as possible agents for the prevention and treatment of various types of malignancies. From this standpoint, the primary objective of this work was to investigate the anticancer properties of Lactiplantibacillus plantarum (LP) and elucidate its underlying mechanism of action. In order to investigate this matter, several doses of LP (ranging from 105 to 1010 CFU/mL) were examined in relation to melanoma cancer cell lines (A375) and breast cancer cell line (MCF-7). The cell viability findings, which were substantiated by morphological investigations and annexin V/PI assay, indicated that LP exerted inhibitory effects on cellular activity and triggered apoptosis. Additionally, upon further investigation into its mechanism, it was observed through the apoptosis assay and Western blot analysis that the administration of LP resulted in an elevation of pro-apoptotic BAX protein levels and an upregulation of cleaved poly-ADP-ribose polymerase (PARP) protein expression. Conversely, the levels of anti-apoptotic Bcl-2 protein were found to decrease in the A375 and MCF-7 cell lines. These findings provide insight into the pro-apoptotic mechanism of action of LP in these specific cell lines.
Microbiologic Findings in a Cohort of Patients with Erythema Migrans
Authors: Ružić-Sabljić Eva , Maraspin Vera , Bogovič Petra , Rojko Tereza , Ogrinc Katarina , Jaklič Martina , Strle Franc
Published: January 2024

Erythema migrans (EM) is the initial and the most frequent clinical manifestation of Lyme borreliosis (LB). Herein, we report on the capacity of culture and serology for the demonstration of Borrelia infection in a cohort of 292 patients diagnosed with typical EM at a single medical center. The median duration of EM at diagnosis was 12 days, and the largest diameter was 16 cm; 252 (86.3%) patients presented with solitary EM, whereas 40 (13.7%) had multiple EM. A total of 95/292 (32.5%) patients had positive IgM, and 169 (57.9%) had positive IgG serum antibodies; the Borrelia isolation rate was 182/292 (62.3%). The most frequent species by far was B. afzelii (142/148, 95.9%) while B. garinii (2.7%) and B. burgdorferi s.s. (1.4%) were rare. IgM seropositivity was associated with a younger age, multiple EM and the absence of underlying chronic illness; IgG seropositivity was associated with the duration of EM at diagnosis, the diameter of the EM, having had a previous episode of LB and the absence of symptoms at the site of the EM. Furthermore, the Borrelia isolation rate was statistically significantly lower in patients with positive Borrelia IgM antibodies. Although microbiologic analyses are not needed for the diagnosis of typical EM, they enable insights into the etiology and dynamics of the immune response in the course of early LB.
Diversity of Culturable Bacteria from the Coral Reef Areas in the South China Sea and Their Agar-Degrading Abilities
Authors: Liu Mei , Yin Fu , Zhao Wenbin , Tian Peng , Zhou Yi , Jia Zhiyu , Huang Keyi , Ding Yunqi , Xiao Jiaguang , Niu Wentao , Wang Xiaolei
Published: January 2024

The South China Sea (SCS) is abundant in marine microbial resources with high primary productivity, which is crucial for sustaining the coral reef ecosystem and the carbon cycle. Currently, research on the diversity of culturable bacteria in the SCS is relatively extensive, yet the culturable bacteria in coral reefs has been poorly understood. In this study, we analyzed the bacterial community structure of seawater samples among Daya Bay (Fujian Province), Qionghai (Hainan Province), Xisha Islands, and the southern South China Sea based on culturable methods and detected their abilities for agar degradation. There were 441 bacterial strains, belonging to three phyla, five classes, 43 genera, and 101 species, which were isolated by marine agar 2216E (MA; Becton Dickinson). Strains within Gammaproteobacteria were the dominant group, accounting for 89.6% of the total bacterial isolates. To investigate vibrios, which usually correlated with coral health, 348 isolates were obtained from TCBS agar, and all isolates were identified into three phylum, three classes, 14 orders, 25 families, and 48 genera. Strains belonging to the genus Vibrio had the greatest number (294 strains), indicating the high selectivity of TCBS agar for vibrios. Furthermore, nineteen strains were identified as potentially novel species according to the low 16S rRNA gene similarity (<98.65%), and 28 strains (15 species) had agar-degrading ability. These results indicate a high diversity of culturable bacteria in the SCS and a huge possibility to find novel and agar-degrading species. Our study provides valuable microbial resources to maintain the stability of coral ecosystems and investigate their roles in the marine carbon cycle.
Antioxidant and Antimelanogenic Activities of Lactobacillus kunkeei NCHBL-003 Isolated from Honeybees
Authors: Lee Yeon-Ji , Choi Joo-Hee , Kang Kyung-Ku , Sung Soo-Eun , Lee Sijoon , Sung Minkyoung , Seo Min-Soo , Park Jong-Hwan
Published: January 2024

Excessive reactive oxygen species production can detrimentally impact skin cell physiology, resulting in cell growth arrest, melanogenesis, and aging. Recent clinical studies have found that lactic acid bacteria have a special effect directly or indirectly on skin organs, but the exact mechanism has not been elucidated. In this study, we investigated the mechanisms underlying the antioxidant protective effect and the inhibitory effect on melanin synthesis of Lactobacillus kunkeei culture supernatant (CSK), isolated from Apis mellifera Linnaeus (the Western honeybee). CSK exhibited notable efficacy in promoting cell migration and wound healing under oxidative stress, surpassing the performance of other strains. CSK pretreatment significantly upregulated the expression of Nrf2/HO-1 (nuclear factor erythroid 2-related factor 2/heme oxygenase-1), a key player in cellular defenses against oxidative stress, relative to the control H2O2-treated cells. The DCF-DA (dichloro-dihydro-fluorescein diacetate) assay results confirmed that CSK’s ability to enhance Nrf2 and HO-1 expression aligns with its robust ability to remove H2O2-induced reactive oxygen species. Furthermore, CSK upregulated MAPK (mitogen-activated protein kinase) phosphorylation, an upstream signal for HO-1 expression, and MAPK inhibitors compromised the wound-healing effect of CSK. Additionally, CSK exhibited inhibitory effects on melanin synthesis, downregulating melanogenesis-related genes in B16F10 cells. Thus, the present study demonstrated that CSK exhibited antioxidant effects by activating the Nrf2/HO-1 pathway through MAPK phosphorylation, thereby restoring cell migration and demonstrating inhibitory effects on melanin production. These findings emphasize the antioxidant and antimelanogenic potential of CSK, suggesting its potential use as a therapeutic agent, promoting wound healing, and as an active ingredient in skin-lightening cosmetics.
The Influence of the Magnesium-to-Phosphate Molar Ratio on Magnesium Potassium Phosphate Cement Properties Using Either Wollastonite or Volcanic Ash as Fillers
Authors: Padilla-Encinas Pilar , Dieguez Mikel , Cuevas Jaime , Ruiz Ana Isabel , Fernández Raúl
Published: January 2024

The use of the fillers wollastonite and volcanic ash for the formulation of magnesium phosphate cements prepared at magnesium-to-phosphate molar ratios of 2, 3 and 4 has been investigated, with the objective of evaluating these formulations for the encapsulation of aluminium radioactive waste. The workability, mechanical strength, dimensional stability, pH, chemical composition and mineralogical properties of cement pastes and mortars were examined. All cement pastes presented fast setting, and the workability was only good at 3 and 4 M. The cement mortars presented high compressive strength and dimensional stability. K-struvite was confirmed as the sole reaction product of the reaction for all formulations. The pH of the cement pastes, measured in suspensions, achieved values in the range of 7.8 to 9.5 after the first days of setting, exceeding pH 8.5 for the 2 and 3 M formulations. pH values below 8.5 are theoretically preferred to avoid potential aluminium corrosion. Both fillers presented adequate characteristics (good workability, chemical compatibility) to be used in the formulation of magnesium phosphate cements. The increasing magnesium-to-phosphate molar ratio prevented unwanted efflorescence and increased the mechanical stability of the cement.
Health-Promoting Properties of Anthocyanins from Cornelian Cherry (Cornus mas L.) Fruits
Authors: Szot Iwona , Łysiak Grzegorz P. , Sosnowska Bożena , Chojdak-Łukasiewicz Justyna
Published: January 2024

The cornelian cherry is a plant that annually provides fruits, drupe-type, ranging in color from yellow through pink, red, carmine, and almost black. Cornelian cherry bears abundant fruit in temperate climate conditions, which means that its dark-colored fruits can be treated as an excellent source of anthocyanins. After consuming, anthocyanins have a protective function in the human body. Raw fruit extracts and their pure isolates, rich in anthocyanins, have a wide spectrum of health-promoting properties. This review focuses on the health-promoting properties of anthocyanins from fruits of cornelian cherry, documented in research conducted in vitro, in vivo, and in humans. The results obtained so far confirm the beneficial effects of anthocyanins on the blood parameters, whose values are important in predicting and assessing the risk and progression of cardiovascular and metabolic diseases. A beneficial effect on molecular and histopathological changes in target organs such as the heart, brain, kidneys, and liver has also been demonstrated. Anthocyanins from cornelian cherry have a strong antioxidant effect, which explains their protective effects on organs and anticancer effects. Moreover, they have antiglycemic, antihyperlipidemic, anti-inflammatory, and antimicrobial properties. The work highlights the perspectives and directions of necessary research.
Organic Transistors Based on Highly Crystalline Donor–Acceptor π-Conjugated Polymer of Pentathiophene and Diketopyrrolopyrrole
Authors: Ren Shiwei , Wang Zhuoer , Chen Jinyang , Wang Sichun , Yi Zhengran
Published: January 2024

Oligomers and polymers consisting of multiple thiophenes are widely used in organic electronics such as organic transistors and sensors because of their strong electron-donating ability. In this study, a solution to the problem of the poor solubility of polythiophene systems was developed. A novel π-conjugated polymer material, PDPP-5Th, was synthesized by adding the electron acceptor unit, DPP, to the polythiophene system with a long alkyl side chain, which facilitated the solution processing of the material for the preparation of devices. Meanwhile, the presence of the multicarbonyl groups within the DPP molecule facilitated donor–acceptor interactions in the internal chain, which further improved the hole-transport properties of the polythiophene-based material. The weak forces present within the molecules that promoted structural coplanarity were analyzed using theoretical simulations. Furthermore, the grazing incidence wide-angle X-ray scanning (GIWAXS) results indicated that PDPP-5Th features high crystallinity, which is favorable for efficient carrier migration within and between polymer chains. The material showed hole transport properties as high as 0.44 cm2 V−1 s−1 in conductivity testing. Our investigations demonstrate the great potential of this polymer material in the field of optoelectronics.
Synthesis of Indenones via Persulfate Promoted Radical Alkylation/Cyclization of Biaryl Ynones with 1,4-Dihydropyridines
Authors: Wang Wanwan , Yu Lei
Published: January 2024

The oxidative radical cascade cyclization of alkynes has emerged as a versatile strategy for the efficient construction of diverse structural units and complex molecules in organic chemistry. This work reports an alkyl radical initiated 5-exo-trig cyclization of biaryl ynones with 1,4-dihydropyridines to selectively synthesize indenones.
Isolation and Structure Elucidation of New Metabolites from the Mariana-Trench-Associated Fungus Aspergillus sp. SY2601
Authors: Sun Cangzhu , Ha Yura , Liu Xin , Wang Nan , Lian Xiao-Yuan , Zhang Zhizhen
Published: January 2024

Fungi are important resource for the discovery of novel bioactive natural products. This study investigated the metabolites produced by Mariana-Trench-associated fungus Aspergillus sp. SY2601 in EY liquid and rice solid media, resulting in the isolation and structure determination of 28 metabolites, including five new compounds, asperindopiperazines A–C (1–3), 5-methoxy-8,9-dihydroxy-8,9-deoxyaspyrone (21), and 12S-aspertetranone D (26). Structures of the new compounds were elucidated based on extensive NMR spectral analyses, HRESIMS data, optical rotation, ECD, and 13C NMR calculations. The new compound 12S-aspertetranone D (26) exhibited antibacterial activity against both methicillin-resistant Staphylococcus aureus and Escherichia coli with MIC values of 3.75 and 5 μg/mL, respectively.