bluuebunny/crossref_metadata_2025 huggingface dataset:
Schistosoma mansoni Infection Is Impacted by Malnutrition
Authors: Maciel Poliane Silva , Gonçalves Ricardo , Antonelli Lis Ribeiro do Valle , Fonseca Cristina Toscano
Published: March 2021

Schistosomiasis remains one of the most important neglected tropical diseases in the world. It mainly affects developing countries, where it often coexists with malnutrition. Despite this, few studies have investigated the relationship between schistosomiasis and malnutrition. Herein, we evaluate the impact of malnutrition on experimentalS. mansoniinfection. Mice were divided into 5 groups: Control (Ctrl) diet (14% protein and 10% lipids), low-protein 3% (LP 3%), low-protein 8% (LP 8%), low-fat 2.5% (LF 2.5%), and low-fat 5% (LF 5%). Mice were fed with their respective diets and were infected when a difference of approximately 20% in the body weight between mice from any experimental group and mice from the control group was achieved. Nutritional, parasitological, and immunological parameters were assessed either just before infection and/or approximately 50 days later before mice were perfused. Our results showed that the 3% low-protein diet was the only one capable of establishing malnutrition in mice. Mice fed with this diet showed: (i) significant reduction in body weight and serum albumin levels before infection, (ii) decreased levels of all biochemical parameters evaluated before perfusion, (iii) decreased numbers of schistosome eggs trapped in intestines and impaired parasite fecundity, (iv) a delay in the granuloma development with a smaller granuloma area, and (v) reduced levels of IL-4 and IFN-γ in the liver. Our findings demonstrate that low protein supply leads to malnutrition in mice and impacts the cytokine milieu in the liver and granuloma formation. Additionally, the establishment of our murine malnutrition model will enable future studies aiming to better understand the complex relationships between nutrition, immune responses, and infection outcome.
Circular RNAs in the Central Nervous System
Authors: Li Meng-Lan , Wang Wen , Jin Zi-Bing
Published: March 2021

Circular RNAs (circRNAs) are endogenous single-stranded RNAs characterized by covalently closed loop structures with neither 5′ to 3′ polarity nor poly(A) tails. They are generated most commonly from back-splicing of protein-coding exons. CircRNAs have a tissue-specific distribution and are evolutionarily conserved, and many circRNAs play important biological functions by combining with microRNAs and proteins to regulate protein functions and their own translation. Numerous studies have shown that circRNAs are enriched in the central nervous system (CNS) and play an important role in the development and maintenance of homeostasis. Correspondingly, they also play an important role in the occurrence and progression of CNS diseases. In this review, we highlight the current state of circRNA biogenesis, properties, function and the crucial roles they play in the CNS.
In vivo N-Terminomics Highlights Novel Functions of ADAMTS2 and ADAMTS14 in Skin Collagen Matrix Building
Authors: Leduc Cédric , Dupont Laura , Joannes Loïc , Monseur Christine , Baiwir Dominique , Mazzucchelli Gabriel , Deroanne Christophe , Colige Alain , Bekhouche Mourad
Published: March 2021

A disintegrin and metalloproteinase with thrombospondin type I motif (ADAMTS)2 and ADAMTS14 were originally known for their ability to cleave the aminopropeptides of fibrillar collagens. Previous work using N-terminomic approach (N-TAILS) in vitro led to the identification of new substrates, including some molecules involved in TGF-β signaling. Here, N-TAILS was used to investigate the substrates of these two enzymes in vivo, by comparing the N-terminomes of the skin of wild type mice, mice deficient in ADAMTS2, in ADAMTS14 and in both ADAMTS2 and ADAMTS14. This study identified 68 potential extracellular and cell surface proteins, with the majority of them being cleaved by both enzymes. These analyses comfort their role in collagen matrix organization and suggest their implication in inflammatory processes. Regarding fibrillar collagen, this study demonstrates that both ADAMTS2 and ADAMTS14 are involved in the processing of the aminopropeptide of alpha1 and alpha2 type V collagen. It also revealed the existence of several cleavage sites in the Col1 domain and in the C-propeptide of type I collagens. In addition to collagens and other extracellular proteins, two major components of the cell cytoskeleton, actin and vimentin, were also identified as potential substrates. The latter data were confirmed in vitro using purified enzymes and could potentially indicate other functions for ADAMTS2 and 14. This original investigation of mouse skin degradomes by N-terminomic highlights the essential role of ADAMTS2 and ADAMTS14 in collagen matrix synthesis and turnover, and gives clues to better understand their functions in skin pathophysiology. Data are available via ProteomeXchange with identifier PXD022179.
Paper Notebooks vs. Mobile Devices: Brain Activation Differences During Memory Retrieval
Authors: Umejima Keita , Ibaraki Takuya , Yamazaki Takahiro , Sakai Kuniyoshi L.
Published: March 2021

It remains to be determined how different inputs for memory-encoding, such as the use of paper notebooks or mobile devices, affect retrieval processes. We compared three groups of participants who read dialogues on personal schedules and wrote down the scheduled appointments on a calendar using a paper notebook (Note), an electronic tablet (Tablet), or a smartphone (Phone). After the retention period for an hour including an interference task, we tested recognition memory of those appointments with visually presented questions in a retrieval task, while scanned with functional magnetic resonance imaging. We obtained three major results. First, the duration of writing down schedules was significantly shorter for the Note group than the Tablet and Phone groups, and accuracy was much higher for the Note group in easier (i.e., more straightforward) questions. Because the input methods were equated as much as possible between the Note and Tablet groups, these results indicate that the cognitive processes for the Note group were deeper and more solid. Second, brain activations for all participants during the retrieval phase were localized in the bilateral hippocampus, precuneus, visual cortices, and language-related frontal regions, confirming the involvement of verbalized memory retrieval processes for appointments. Third, activations in these regions were significantly higher for the Note group than those for the Tablet and Phone groups. These enhanced activations for the Note group could not be explained by general cognitive loads or task difficulty, because overall task performances were similar among the groups. The significant superiority in both accuracy and activations for the Note group suggested that the use of a paper notebook promoted the acquisition of rich encoding information and/or spatial information of real papers and that this information could be utilized as effective retrieval clues, leading to higher activations in these specific regions.
Utility of 7 Tesla Magnetic Resonance Imaging in Patients With Epilepsy: A Systematic Review and Meta-Analysis
Authors: Park Ji Eun , Cheong E-Nae , Jung Da Eun , Shim Woo Hyun , Lee Ji Sung
Published: March 2021

Objective: 7 Tesla magnetic resonance imaging (MRI) enables high resolution imaging and potentially improves the detection of morphologic abnormalities in patients with epilepsy. However, its added value compared with conventional 1.5T and 3.0T MRI is unclear. We reviewed the evidence for the use of 7 Tesla MRI in patients with epilepsy and compared the detection rate of focal lesions with clinical MRI.

Methods: Clinical retrospective case studies were identified using the indexed text terms “epilepsy” AND “magnetic resonance imaging” OR “MR imaging” AND “7T” OR “7 Tesla” OR “7T” in Medline (2002-September 1, 2020) and Embase (1999-September 1, 2020). The study setting, MRI protocols, qualitative, and quantitative assessment were systematically reviewed. The detection rate of morphologic abnormalities on MRI was reported in each study in which surgery was used as the reference standard. Meta-analyses were performed using a univariate random-effects model in diagnostic performance studies with patients that underwent both 7T MRI and conventional MRI.

Results: Twenty-five articles were included (467 patients and 167 healthy controls) consisting of 10 case studies, 10 case-control studies, 4 case series, and 1 cohort study. All studies included focal epilepsy; 12 studies (12/25, 48%) specified the disease etiology and 4 studies reported focal but non-lesional (MRI-negative on 1.5/3.0T) epilepsy. 7T MRI showed superior detection and delineation of morphologic abnormalities in all studies. In nine comparative studies, 7T MRI had a superior detection rate of 65% compared with the 22% detection rate of 1.5T or 3.0T.

Significance: 7T MRI is useful for delineating morphologic abnormalities with a higher detection rate compared with conventional clinical MRI. Most studies were conducted using a case series or case study; therefore, a cohort study design with clinical outcomes is necessary.

Classification of Evidence: Class IV Criteria for Rating Diagnostic Accuracy Studies.
Analysis of Approaches in the Microsurgical Treatment of 102 Cases of Petroclival Meningioma in a Single Center
Authors: Lin Yazhou , Gao Qiang , Jin Huiping , Wang Nana , Xu Dingkang , Wang Fang , Guo A. Bao , Zang Weidong , Li Zhihua , Guo Fuyou
Published: March 2021

Objectives: We identified the optimal approaches for treating the diverse tumor subtypes of petroclival meningioma (PM) by analyzing the clinical benefits of various surgical approaches adopted for each subtype.

Methods: Tumors in 102 PM patients from a single center who underwent surgical treatment were classified as upper clivus (UC), cavernous sinus (CS), tentorium (TE), or petrous apex (PA) types based on the attachment site of the tumor base and the displacement of the trigeminal nerve. The therapeutic effects of different surgical approaches among the subtypes were evaluated according to the patient outcomes.

Results: The subtemporal (33.33%), retrosigmoid (16.67%), and Kawase approaches (50%) were used for the UC type. Simpson I/II resection was achieved in 46.66% of patients with the Kawase approach. Significant differences were found between the other two approaches (P = 0.044) and in the follow-up Karnofsky performance scale (KPS) scores (P = 0.008). The subtemporal (60%) and Kawase approaches (40%) were used for the CS type; neither approach achieved Simpson I/II resection. The retrosigmoid (25.81%) and Kawase approaches (74.19%) were used for the TE type. The Simpson I/II resection rates of the two approaches were 55.55 and 86.95%, respectively, and a significant difference was observed between them (P = 0.039). The retrosigmoid (43.75%) and Kawase approaches (56.25%) were used for the PA type. The Simpson I/II resection rates of the two approaches were 31.25 and 50%, respectively. The resection degrees of the two approaches and the KPS scores at follow-up were significantly different (P = 0.034).

Conclusion: The individual microsurgical approaches adopted for the various PM tumor subtypes can provide maximal safe resection and good KPS scores. The Kawase approach is more suitable for PM, especially for UC- and PA-type PM tumors.
Drug Repurposing for Parkinson’s Disease: The International Linked Clinical Trials experience
Authors: Stott Simon R. W. , Wyse Richard K. , Brundin Patrik
Published: March 2021

The international Linked Clinical Trials (iLCT) program for Parkinson’s to date represents one of the most comprehensive drug repurposing programs focused on one disease. Since initial planning in 2010, it has rapidly grown – giving rise to seven completed, and 15 ongoing, clinical trials of 16 agents each aimed at delivering disease modification in Parkinson’s disease (PD). In this review, we will provide an overview of the history, structure, process, and progress of the program. We will also present some examples of agents that have been selected and prioritized by the program and subsequently evaluated in clinical trials. Our goal with this review is to provide a template that can be considered across other therapeutic areas.
Applications of Non-invasive and Novel Methods of Low-Field Nuclear Magnetic Resonance and Magnetic Resonance Imaging in Aquatic Products
Authors: Wang Xin-Yun , Xie Jing , Chen Xin-Jun
Published: March 2021

Aquatic products, such as fish, are popular throughout the world due to their satisfying flavor characteristics as well as rich animal nutrition, and they provide high-value food therapy, but they are easily oxidized and spoiled. It is necessary to detect aquatic products through rapid and accurate technology. Low-field nuclear magnetic resonance (LF-NMR) and magnetic resonance imaging (MRI) have been widely used in the aquatic product industry due to their sensitivity, fast analysis, non-destructive nature and low cost. The applications of LF-NMR in the measurement of aquatic product quality and nutrients (water, fat, and protein) are summarized in this paper. Applications in aquatic products have been shown to depend on deep processing, storage and authentication. This review discusses the application of MRI technology in the quality control of aquatic products. Therefore, this review will guide the application of the aquatic products industry and aims to supply the reader with both the theory of the method and practical applications of the method for use as a rapid and non-destructive technology in scientific research and the industrial industry.
HOTAIRM1 Promotes Malignant Progression of Transformed Fibroblasts in Glioma Stem-Like Cells Remodeled Microenvironment via Regulating miR-133b-3p/TGFβ Axis
Authors: Wang Haiyang , Li Haoran , Jiang Qianqian , Dong Xuchen , Li Suwen , Cheng Shan , Shi Jia , Liu Liang , Qian Zhiyuan , Dong Jun
Published: March 2021

Recent studies have reported that cancer associated fibroblasts (CAFs) and glioma stem-like cells (GSCs) played active roles in glioma progression in tumor microenvironment (TME). Long non-coding RNAs (lncRNAs) have been found to be closely associated with glioma development in recent years, however, their molecular regulatory mechanisms on CAFs in GSCs remodeled TME kept largely unelucidated. Our study found that GSCs could induce malignant transformation of fibroblasts (t-FBs) based on dual-color fluorescence tracing orthotopic model. Associated with poor prognosis, Lnc HOXA transcript antisense RNA, myeloid-specific 1 (HOTAIRM1) was highly expressed in high-grade gliomas and t-FBs. Depleting HOTAIRM1 inhibited the proliferation, invasion, migration, and even tumorigenicity of t-FB. Conversely, overexpression of HOTAIRM1 promoted malignancy phenotype of t-FB. Mechanistically, HOTAIRM1 directly bound with miR-133b-3p, and negatively regulated the latter. MiR-133b-3p partly decreased the promotion effect of HOTAIRM1 on t-FBs. Furthermore, transforming growth factor-β (TGFβ) was verified to be a direct target of miR-133b-3p. HOTAIRM1 can modulate TGFβ via competing with miR-133b-3p. Collectively, HOTAIRM1/miR-133b-3p/TGFβ axis was involved in modulating t-FBs malignancy in TME remodeled by GSCs, which had the potential to serve as a target against gliomas.
A Modular System for Treating Moving Anatomical Targets With Scanned Ion Beams at Multiple Facilities: Pre-Clinical Testing for Quality and Safety of Beam Delivery
Authors: Lis Michelle , Newhauser Wayne , Donetti Marco , Wolf Moritz , Steinsberger Timo , Paz Athena , Durante Marco , Graeff Christian
Published: March 2021

Background

Quality management and safety are integral to modern radiotherapy. New radiotherapy technologies require new consensus guidelines on quality and safety. Established analysis strategies, such as the failure modes and effects analysis (FMEA) and incident learning systems have been developed as tools to assess the safety of several types of radiation therapies. An extensive literature documents the widespread application of risk analysis methods to photon radiation therapy. Relatively little attention has been paid to performing risk analyses of nascent radiation therapy systems to treat moving tumors with scanned heavy ion beams. The purpose of this study was to apply a comprehensive safety analysis strategy to a motion-synchronized dose delivery system (M-DDS) for ion therapy.

Methods

We applied a risk analysis method to new treatment planning and treatment delivery processes with scanned heavy ion beams. The processes utilize a prototype, modular dose delivery system, currently undergoing preclinical testing, that provides new capabilities for treating moving anatomy. Each step in the treatment process was listed in a process map, potential errors for each step were identified and scored using the risk probability number in an FMEA, and the possible causes of each error were described in a fault tree analysis. Solutions were identified to mitigate the risk of these errors, including permanent corrective actions, periodic quality assurance (QA) tests, and patient specific QA (PSQA) tests. Each solution was tested experimentally.

Results

The analysis revealed 58 potential errors that could compromise beam delivery quality or safety. Each of the 14 binary (pass-or-fail) tests passed. Each of the nine QA and four PSQA tests were within anticipated clinical specifications. The modular M-DDS was modified accordingly, and was found to function at two centers.

Conclusion

We have applied a comprehensive risk analysis strategy to the M-DDS and shown that it is a clinically viable motion mitigation strategy. The described strategy can be utilized at any ion therapy center that operates with the modular M-DDS. The approach can also be adapted for use at other facilities and can be combined with existing safety analysis systems.
Emerging Biomarkers for Predicting Bladder Cancer Lymph Node Metastasis
Authors: Zhang Chunyu , Hu Jiao , Li Huihuang , Ma Hongzhi , Othmane Belaydi , Ren Wenbiao , Yi Zhenglin , Qiu Dongxu , Ou Zhenyu , Chen Jinbo , Zu Xiongbing
Published: March 2021

Bladder cancer is one of the leading causes of cancer deaths worldwide. Early detection of lymph node metastasis of bladder cancer is essential to improve patients’ prognosis and overall survival. Current diagnostic methods are limited, so there is an urgent need for new specific biomarkers. Non-coding RNA and m6A have recently been reported to be abnormally expressed in bladder cancer related to lymph node metastasis. In this review, we tried to summarize the latest knowledge about biomarkers, which predict lymph node metastasis in bladder cancer and their mechanisms. In particular, we paid attention to the impact of non-coding RNA on lymphatic metastasis of bladder cancer and its specific molecular mechanisms, as well as some prediction models based on imaging, pathology, and biomolecules, in an effort to find more accurate diagnostic methods for future clinical application.
Expression of TMEM16A in Colorectal Cancer and Its Correlation With Clinical and Pathological Parameters
Authors: Li Hongxia , Yang Qiwei , Huo Sibo , Du Zhenwu , Wu Fei , Zhao Haiyue , Chen Shifan , Yang Longfei , Ma Zhiming , Sui Yujie
Published: March 2021

TMEM16A is a recently identified calcium-activated chloride channel (CaCC) and its overexpression contributes to tumorigenesis and progression in several human malignancies. However, little is known about expression of TMEM16A and its clinical significance in colorectal cancer (CRC). TMEM16A mRNA expression was determined by quantitative real time-PCR (qRT-PCR) in 67 CRC tissues and 24 para-carcinoma tissues. TMEM16A protein expression was performed by immunohistochemistry in 80 CRC tissues. The correlation between TMEM16A expression and clinicopathological parameters, and known genes and proteins involved in CRC was analyzed. The results showed that TMEM16A mRNA expression was frequently detected in 51 CRC tissues (76%), whereas TMEM16A protein expression was determined at a relatively lower frequency (26%). TMEM16A mRNA expression in tumor tissues was higher than its expression in normal para-carcinoma tissues (P < 0.05). TMEM16A mRNA expression was significantly correlated with TNM stage (p = 0.039) and status of lymph node metastasis (p = 0.047). In addition, there was a strong positive correlation between TMEM16A mRNA expression and MSH2 protein. More importantly, TMEM16A protein expression was positively associated with KRAS mutation, and negatively correlated with mutant p53 protein. Logistic regression analysis demonstrated that TMEM16A mRNA expression was an important independent predictive factor of lymph node metastasis (OR = 16.38, CI: 1.91–140.27, p = 0.01). TMEM16A mRNA and protein expression was not significantly related with patient survival. Our findings provide original evidence demonstrating TMEM16A mRNA expression can be a novel predictive marker of lymph node metastasis and TMEM16A protein expression may be an important regulator of tumor proliferation and metastasis in CRC.
A Practical Nomogram to Predict Early Death in Advanced Epithelial Ovarian Cancer
Authors: Song Zixuan , Zhou Yangzi , Bai Xue , Zhang Dandan
Published: March 2021

Background: Ovarian cancer is a common gynecological malignancy, most of which is epithelial ovarian cancer (EOC). Advanced EOC is linked with a higher incidence of premature death. To date, no effective prognostic tools are available to evaluate the possibility of early death in patients with advanced EOC.

Methods: Advanced (FIGO stage III and IV) EOC patients who were enrolled in the Surveillance, Epidemiology, and End Results database between 2004 and 2015 were regarded as subjects and studied. We aimed to construct a nomogram that can deliver early death prognosis in patients with advanced EOC by identifying crucial independent factors using univariate and multivariate logistic regression analyses to help deliver accurate prognoses.

Results: In total, 13,403 patients with advanced EOC were included in this study. Three hundred ninety-seven out of a total of 9,379 FIGO stage III patients died early. There were 4,024 patients with FIGO stage IV, 414 of whom died early. Nomograms based on independent prognostic factors have the satisfactory predictive capability and clinical pragmatism. The internal validation feature of the nomogram demonstrated a high level of accuracy of the predicted death.

Conclusions: By analyzing data from a large cohort, a clinically convenient nomogram was established to predict premature death in advanced EOC. This tool can aid clinicians in screening patients who are at higher risk for tailoring treatment plans.
Cyclovirobuxine D Induces Apoptosis and Mitochondrial Damage in Glioblastoma Cells Through ROS-Mediated Mitochondrial Translocation of Cofilin
Authors: Zhang Lin , Fu Ruoqiu , Duan Dongyu , Li Ziwei , Li Bin , Ming Yue , Li Li , Ni Rui , Chen Jianhong
Published: March 2021

Background

Cyclovirobuxine D (CVBD), a steroidal alkaloid, has multiple pharmacological activities, including anti-cancer activity. However, the anti-cancer effect of CVBD on glioblastoma (GBM) has seldom been investigated. This study explores the activity of CVBD in inducing apoptosis of GBM cells, and examines the related mechanism in depth.

Methods

GBM cell lines (T98G, U251) and normal human astrocytes (HA) were treated with CVBD. Cell viability was examined by CCK-8 assay, and cell proliferation was evaluated by cell colony formation counts. Apoptosis and mitochondrial superoxide were measured by flow cytometry. All protein expression levels were determined by Western blotting. JC-1 and CM-H2DCFDA probes were used to evaluate the mitochondrial membrane potential (MMP) change and intracellular ROS generation, respectively. The cell ultrastructure was observed by transmission electron microscope (TEM). Colocalization of cofilin and mitochondria were determined by immunofluorescence assay.

Results

CVBD showed a greater anti-proliferation effect on the GBM cell lines, T98G and U251, than normal human astrocytes in dose- and time-dependent manners. CVBD induced apoptosis and mitochondrial damage in GBM cells. We found that CVBD led to mitochondrial translocation of cofilin. Knockdown of cofilin attenuated CVBD-induced apoptosis and mitochondrial damage. Additionally, the generation of ROS and mitochondrial superoxide was also induced by CVBD in a dose-dependent manner. N-acetyl-L-cysteine (NAC) and mitoquinone (MitoQ) pre-treatment reverted CVBD-induced apoptosis and mitochondrial damage. MitoQ pretreatment was able to block the mitochondrial translocation of cofilin caused by CVBD.

Conclusions

Our data revealed that CVBD induced apoptosis and mitochondrial damage in GBM cells. The underlying mechanism is related to mitochondrial translocation of cofilin caused by mitochondrial oxidant stress.
Defining Immunogenic and Radioimmunogenic Tumors
Authors: Medler Terry R. , Blair Tiffany C. , Crittenden Marka R. , Gough Michael J.
Published: March 2021

In the cancer literature tumors are inconsistently labeled as ‘immunogenic’, and experimental results are occasionally dismissed since they are only tested in known ‘responsive’ tumor models. The definition of immunogenicity has moved from its classical definition based on the rejection of secondary tumors to a more nebulous definition based on immune infiltrates and response to immunotherapy interventions. This review discusses the basis behind tumor immunogenicity and the variation between tumor models, then moves to discuss how these principles apply to the response to radiation therapy. In this way we can identify radioimmunogenic tumor models that are particularly responsive to immunotherapy only when combined with radiation, and identify the interventions that can convert unresponsive tumors so that they can also respond to these treatments.
A Comparison of Three Ways to Measure Time-Dependent Densities With Quantum Simulators
Authors: Yang Jun , Brown James , Whitfield James Daniel
Published: March 2021

Quantum algorithms are touted as a way around some classically intractable problems such as the simulation of quantum mechanics. At the end of all quantum algorithms is a quantum measurement whereby classical data is extracted and utilized. In fact, many of the modern hybrid-classical approaches are essentially quantum measurements of states with short quantum circuit descriptions. Here, we compare and examine three methods of extracting the time-dependent one-particle probability density from a quantum simulation: direct Z-measurement, Bayesian phase estimation, and harmonic inversion. We have tested these methods in the context of the potential inversion problem of time-dependent density functional theory. Our test results suggest that direct measurement is the preferable method. We also highlight areas where the other two methods may be useful and report on tests using Rigetti's quantum virtual device. This study provides a starting point for imminent applications of quantum computing.
Boosting and Taming Wave Breakup in Second Harmonic Generation
Authors: Jauberteau Raphaël , Wehbi Sahar , Mansuryan Tigran , Krupa Katarzyna , Baronio Fabio , Wetzel Benjamin , Tonello Alessandro , Wabnitz Stefan , Couderc Vincent
Published: March 2021

Modulation instability is a universal phenomenon that can be found in a wide variety of nonlinear systems where, in the presence of a noise seed, peaks of random intensities can be generated. Several dynamical systems admit exact solutions in the form of breathers or solitons on a finite background. The vast majority of soliton studies has been restricted so far to one-dimensional systems. In contrast, the occurrences of localized structures in fully spatiotemporal systems has been only sporadically explored. In this work, we experimentally study the conditions for the wave-breaking of spatially extended optical beams in the process of second harmonic generation. Whenever the pump energy of the picosecond-long fundamental beam reaches a critical level, the beam shape at the second harmonic in a KTP crystal breaks into small filaments. These filaments exhibit extreme local intensity peaks, and their statistical distribution can be modified by the input energy of the fundamental beam. Moreover, by analyzing similar wave-breaking dynamics in a PPLN crystal in the presence of a higher nonlinear quadratic response, we observe that the spatial beam breaking may even gradually vanish as the laser intensity grows larger, leading to a spatial reshaping into a smooth and wider beam, accompanied by a substantial broadening of its temporal spectrum.
Population Genomics of the “Arcanum” Species Group in Wild Tomatoes: Evidence for Separate Origins of Two Self-Compatible Lineages
Authors: Florez-Rueda Ana M. , Scharmann Mathias , Roth Morgane , Städler Thomas
Published: March 2021

Given their diverse mating systems and recent divergence, wild tomatoes (Solanum section Lycopersicon) have become an attractive model system to study ecological divergence, the build-up of reproductive barriers, and the causes and consequences of the breakdown of self-incompatibility. Here we report on a lesser-studied group of species known as the “Arcanum” group, comprising the nominal species Solanum arcanum, Solanum chmielewskii, and Solanum neorickii. The latter two taxa are self-compatible but are thought to self-fertilize at different rates, given their distinct manifestations of the morphological “selfing syndrome.” Based on experimental crossings and transcriptome sequencing of a total of 39 different genotypes from as many accessions representing each species’ geographic range, we provide compelling evidence for deep genealogical divisions within S. arcanum; only the self-incompatible lineage known as “var. marañón” has close genealogical ties to the two self-compatible species. Moreover, there is evidence under multiple inference schemes for different geographic subsets of S. arcanum var. marañón being closest to S. chmielewskii and S. neorickii, respectively. To broadly characterize the population-genomic consequences of these recent mating-system transitions and their associated speciation events, we fit demographic models indicating strong reductions in effective population size, congruent with reduced nucleotide and S-locus diversity in the two independently derived self-compatible species.
Temperature Acclimation of the Picoalga Ostreococcus tauri Triggers Early Fatty-Acid Variations and Involves a Plastidial ω3-Desaturase
Authors: Degraeve-Guilbault Charlotte , Pankasem Nattiwong , Gueirrero Maurean , Lemoigne Cécile , Domergue Frédéric , Kotajima Tomonori , Suzuki Iwane , Joubès Jérôme , Corellou Florence
Published: March 2021

Alteration of fatty-acid unsaturation is a universal response to temperature changes. Marine microalgae display the largest diversity of polyunsaturated fatty-acid (PUFA) whose content notably varies according to temperature. The physiological relevance and the molecular mechanisms underlying these changes are however, still poorly understood. The ancestral green picoalga Ostreococcus tauri displays original lipidic features that combines PUFAs from two distinctive microalgal lineages (Chlorophyceae, Chromista kingdom). In this study, optimized conditions were implemented to unveil early fatty-acid and desaturase transcriptional variations upon chilling and warming. We further functionally characterized the O. tauri ω3-desaturase which is closely related to ω3-desaturases from Chromista species. Our results show that the overall omega-3 to omega-6 ratio is swiftly and reversibly regulated by temperature variations. The proportion of the peculiar 18:5 fatty-acid and temperature are highly and inversely correlated pinpointing the importance of 18:5 temperature-dependent variations across kingdoms. Chilling rapidly and sustainably up-regulated most desaturase genes. Desaturases involved in the regulation of the C18-PUFA pool as well as the Δ5-desaturase appear to be major transcriptional targets. The only ω3-desaturase candidate, related to ω3-desaturases from Chromista species, is localized at chloroplasts in Nicotiana benthamiana and efficiently performs ω3-desaturation of C18-PUFAs in Synechocystis sp. PCC6803. Overexpression in the native host further unveils a broad impact on plastidial and non-plastidial glycerolipids illustrated by the alteration of omega-3/omega-6 ratio in C16-PUFA and VLC-PUFA pools. Global glycerolipid features of the overexpressor recall those of chilling acclimated cells.
Delay of Gratification Predicts Eating in the Absence of Hunger in Preschool-Aged Children
Authors: Giuliani Nicole R. , Kelly Nichole R.
Published: March 2021

Poor ability to regulate one's own food intake based on hunger cues may encourage children to eat beyond satiety, leading to increased risk of diet-related diseases. Self-regulation has multiple forms, yet no one has directly measured the degree to which different domains of self-regulation predict overeating in young children. The present study investigated how three domains of self-regulation (i.e., appetitive self-regulation, inhibitory control, and attentional control) predicted eating in the absence of hunger (EAH) in a community sample of 47 preschool-aged children (Mage = 4.93, SD = 0.86). Appetitive self-regulation, as measured using a delay of gratification task, was significantly and negatively associated with EAH 1 year later (p< 0.5). Measures of inhibitory and attentional control did not significantly predict EAH. These results suggest that food-related self-regulation may be a better predictor of overeating behaviors than general measures of self-regulation.
On Darwin’s Steps: Citizen Science Can Help Keep an Eye on Alien Species
Authors: Giovos Ioannis , Charitou Anastasia , Gervasini Eugenio , López-Cañizares Celia , Tsiamis Konstantinos , Cardoso Ana Cristina
Published: March 2021

Citizens around the world can act like scientists by providing important information about biodiversity that can help scientists’ research. The same applies to species that expand from their areas of origin to other places, often creating problems for the native species. Nowadays, there are plenty of projects that involve citizen scientists in the monitoring of alien species and biodiversity. The European Alien Species Information Network (EASIN) of the European Commission gathers data from several projects and has developed its own app that enables people to report observations of invasive alien species. So, are you ready to become a citizen scientist?
Non-fiber Carbohydrates in Forages and Their Influence on Beef Production Systems
Authors: Villalba Juan J. , Ates Serkan , MacAdam Jennifer W.
Published: March 2021

Forages can provide a complete diet for ruminant animals, increasing the sustainability of beef production systems worldwide while reducing competition with humans for agricultural land or grain crops. Much of the emphasis on the nutritional characteristics of forages has been on the fiber, sugars, starch, and protein they supply to the rumen, despite the fact that other less-explored constituents, i.e., neutral detergent soluble fiber (NDSF) and other non-structural or non-fiber carbohydrates (NFC) also play a key role in the nutrition of ruminants. This paper explores the less investigated potential of temperate legumes to accumulate levels of NFC comparable to corn silage or beet pulp in cool, dry environments under irrigation, and its implications for forage-based beef production systems. We conclude that genetic or managerial interventions (i.e., breeding programs, defoliation frequency) or ecological conditions (i.e., climate, elevation) that increase concentrations of NFC in legumes can enhance beef production, meat quality, and the efficiency of nitrogen utilization by ruminants while reducing environmental impacts.
Kageneckia oblonga Leaves Subjected to Different Drying Methods: Drying Kinetics, Energy Consumption and Interesting Compounds
Authors: Zambra Carlos , Hernández Diógenes , Reyes Hugo , Riveros Nicole , Lemus-Mondaca Roberto
Published: March 2021

In this study, Kageneckia oblonga leaves were dried under different drying conditions and techniques [oven drying (NC), vacuum drying (VNC), convective drying (FC), and microwave-assisted convective drying (MWFC)]. Thus, the effect of temperature, vacuum, and microwave on the drying features of K. oblonga leaves was determined. Fick's second law was used to calculate the effective moisture diffusivity that varied from 3.94 to 8.14 × 10−11 m2/s, 1.12 to 1.40 × 10−11 m2/s, 7.83 to 11.36 × 10−11 m2/s, and 6.93 to 16.72 × 10−11 m2/s for NC, VNC, FC, and MWFC methods, respectively. In addition, the Weibull and Midilli–Kucuk models accurately predicted all experimental drying curves of K. oblonga leaves. Regarding the energy consumption and efficiency values for different drying methods of K. oblonga were found to be in the range of 0.20–7.50 kW·h and 0.10–3.70%, respectively. The results showed that MWFC method does not significantly affect the phenolic compounds and could be used for large-scale production of K. oblonga dried leaves.
The Isolation and Replication of African Swine Fever Virus in Primary Renal-Derived Swine Macrophages
Authors: Oh Taehwan , Do Duy Tien , Vo Hung Van , Kwon Hyeok-il , Lee Seung-Chul , Kim Min Ho , Nguyen Dung Thi Thu , Le Quang Tin Vinh , Tran Tan Minh , Nguyen Toan Tat , Lee Joo Young , Chae Chanhee
Published: March 2021

African swine fever virus (ASFV) causes hemorrhagic disease in domestic pigs by replicating mainly in monocyte/macrophage lineages. Various primary cells including pulmonary alveolar macrophages have been used for the propagation of ASFV on this account. However, ethical constraints and consistency problems exist as it is necessary to harvest same phenotype of primary cells in order to continue a study. We suggested renal-derived swine macrophages as a novel primary cell candidate to address these issues. These primary cells proved to be permissive to both cell adapted ASFV and a wild-type ASFV. Compared to the commercial cell line MA-104, the renal-derived macrophages were more suitable to isolate the field virus. The consistent molecular characteristics of the renal-derived macrophages were demonstrated by immunocytochemistry with antibodies against macrophage cell surface markers including CD163, CD172a, and Iba-1. Viral protein p30 and p72 expression in ASFV infected macrophages was confirmed by immunocytochemistry by use of specific monoclonal antibodies. We observed increase of cell-free viral DNA and infectious virus titer in infected cell supernatant in successive days-post-infection. These results demonstrated that primary renal-derived swine macrophages are useful for ASFV isolation and propagation in terms of cell phenotypes, susceptibility to the virus, and virus production.
Public Preferences for Exit Strategies From COVID-19 Lockdown in Germany—A Discrete Choice Experiment
Authors: Krauth Christian , Oedingen Carina , Bartling Tim , Dreier Maren , Spura Anke , de Bock Freia , von Rüden Ursula , Betsch Cornelia , Korn Lars , Robra Bernt-Peter
Published: March 2021

Objectives: To decrease the rapid growth of SARS-CoV-2 in Germany, a stepped lockdown was conducted. Acceptance and compliance regarding entering and exiting lockdown measures are key for their success. The aim of the present study was to analyse the population's preferences for exiting lockdown measures.

Methods: To evaluate population’s preferences and identify trade-offs between different exit strategies, a discrete choice experiment was conducted on 28–29 April (n = 1,020). Overall, six attributes and 16 choice sets (fractional-factorial design) without an opt-out were chosen. Conditional logit and latent class models were conducted.

Results: Most attributes proved to be significant. Two attributes dominated all others: Avoiding a mandatory tracing app, and providing sufficient intensive care capacities. Preventing a high long-term unemployment rate and avoiding the isolation of persons aged 70+, were relevant, though utilities were comparatively lower. We identified subgroups (elderly persons and persons with school children) with different utilities, which indicates specific attributes affecting them dissimilarly.

Conclusions: The population prefers cautious re-opening strategies and is at least sceptical about the adoption of severe protection measures. Government should balance interests between subgroups.
The Association Between Ideal Cardiovascular Health and Health-Related Quality of Life in Adults: A Population-Based Cross-Sectional Study
Authors: Wu Xueyan , Liu Xiaotian , Liao Wei , Kang Ning , Sang Shengxiang , Abdulai Tanko , Niu Miaomiao , He Yaling , Zhai Zhihan , Pan Mingming , Wang Chongjian , Li Yuqian
Published: March 2021

Objectives: The study aimed to explore the association between Ideal cardiovascular health (ICH) and health-related quality of life (HRQoL) using the European Quality of Life Five Dimension Five Level Scale (EQ-5D-5L) among rural population.

Methods: This study included 20,683 participants aged 18–79 years from “the Henan Rural Cohort study”. Generalized linear and Tobit regression models were employed to explore the associations of ICH with EQ-5D-5L utility scores and visual analogue scale (VAS) scores, respectively.

Results: The mean EQ-5D-5L utility scores and VAS scores were 0.962 ± 0.095 and 79.52 ± 14.02, respectively. Comparing with poor CVH participants (EQ-5D-5L utility scores and VAS scores: 0.954 ± 0.111 and 78.44 ± 14.29), people with intermediate and ideal CVH had higher EQ-5D-5L utility scores (0.969 ± 0.079 and 0.959 ± 0.099) and VAS scores (80.43 ± 13.65 and 79.28 ± 14.14). ICH scores were positively correlated with EQ-5D-5L utility scores (0.007 (0.004, 0.009)) and VAS scores (0.295 (0.143, 0.446)), respectively.

Conclusions: Higher ICH scores is positive associated with better HRQoL in rural population, which suggests that improvement of cardiovascular health may help to enhance HRQoL among rural population.
On the Descriptive Complexity of Color Coding
Authors: Bannach Max , Tantau Till
Published: March 2021

Color coding is an algorithmic technique used in parameterized complexity theory to detect “small” structures inside graphs. The idea is to derandomize algorithms that first randomly color a graph and then search for an easily-detectable, small color pattern. We transfer color coding to the world of descriptive complexity theory by characterizing—purely in terms of the syntactic structure of describing formulas—when the powerful second-order quantifiers representing a random coloring can be replaced by equivalent, simple first-order formulas. Building on this result, we identify syntactic properties of first-order quantifiers that can be eliminated from formulas describing parameterized problems. The result applies to many packing and embedding problems, but also to the long path problem. Together with a new result on the parameterized complexity of formula families involving only a fixed number of variables, we get that many problems lie in FPT just because of the way they are commonly described using logical formulas.
Assessing Seasonal Methane and Nitrous Oxide Emissions from Furrow-Irrigated Rice with Cover Crops
Authors: Karki Sandhya , Adviento-Borbe M. Arlene A. , Massey Joseph H. , Reba Michele L.
Published: March 2021

Improved irrigation management is identified as a potential mitigation option for methane (CH4) emissions from rice (Oryza sativa). Furrow-irrigated rice (FR), an alternative method to grow rice, is increasingly adopted in the Mid-South U.S. However, FR may provide a potential risk to yield performance and higher emissions of nitrous oxide (N2O). This study quantified the grain yields, CH4 and N2O emissions from three different water management practices in rice: multiple-inlet rice irrigation (MIRI), FR, and FR with cereal rye (Secale cereale) and barley (Hordeum vulgare) as preceding winter cover crops (FRCC). CH4 and N2O fluxes were measured from May to September 2019 using a static chamber technique. Grain yield from FR (11.8 Mg ha−1) and MIRI (12.0 Mg ha−1) was similar, and significantly higher than FRCC (8.5 Mg ha−1). FR and FRCC drastically reduced CH4 emissions compared to MIRI. Total seasonal CH4 emissions decreased in the order of 44 > 11 > 3 kg CH4-C ha−1 from MIRI, FR, and FRCC, respectively. Cumulative seasonal N2O emissions were low from MIRI (0.1 kg N2O-N ha−1) but significantly higher from FR (4.4 kg N2O-N ha−1) and FRCC (3.0 kg N2O-N ha−1). However, there was no net difference in global warming potential among FR, FRCC and MIRI. These results suggest that the increased N2O flux from furrow-irrigated rice may not greatly detract from the potential benefits that furrow-irrigation offers rice producers.
Effects of Short-Term Root Cooling before Harvest on Yield and Food Quality of Chinese Broccoli (Brassica oleracea var. Alboglabra Bailey)
Authors: He Fang , Thiele Björn , Kraus David , Bouteyine Souhaila , Watt Michelle , Kraska Thorsten , Schurr Ulrich , Kuhn Arnd Jürgen
Published: March 2021

Vegetable product quality is an important consideration for consumers. Long-term root cooling could improve certain food quality of horticultural crops, but often comes at the expense of reduced shoot biomass or yield. Since few studies have investigated how fast Chinese broccoli (Brassica oleracea var. alboglabra Bailey) responds to changes of root temperature, we shortened the duration of the root cooling treatment to one week before harvest to make the production system more effective. The aim of this study was to improve the food quality of Chinese broccoli without causing deleterious effects on plant growth and yield. The seedlings were cultivated hydroponically at two root temperatures (10 and 20 °C) during the last week prior to harvest in summer 2018 (Exp-1) and autumn 2019 (Exp-2). Plant growth, yield, physiological variables, soluble sugars, total chlorophyll, glucosinolates and mineral elements concentration were examined. The results showed that the yield reduction was alleviated compared to results over the long-term. Specifically, yield was not affected by root cooling in Exp-1 and reduced by 18.9% in Exp-2 compared to 20 °C. Glucose and fructose concentrations of the leaves were increased when the root temperature was 10 °C in both experiments with a more pronounced impact in Exp-2. In addition, root cooling produced a significant accumulation of individual glucosinolates, such as progoitrin, gluconapin, 4-methoxyglucobrassicin and 4-hydroxyglucobrassicin, in the stems of Exp-1 and the leaves of Exp-2. Minerals, such as N, showed reductions in the shoot, but accumulation in the root. Therefore, compared to long-term root cooling, short-term (one week) reduction of the root temperature is more economical and could help improve certain quality characteristics of Chinese broccoli with less or even no yield reduction.