bluuebunny/crossref_metadata_2025 huggingface dataset:
Enhancing spelling competence for English as a foreign language young learners through digital escape rooms
Authors: Saban Marlene , Grünke Matthias , Kahn-Horwitz Janina
Published: November 2024

This study examines the efficacy of using a digital gamed platform in acquiring phoneme-grapheme correspondences of closed syllables with short vowels. Fifty-five fourth-grade English as a Foreign Language (EFL) learners navigated through five digital escape rooms where they learned, practiced, and produced target graphemes. Mixed methods analyses found that spelling competence in the intervention group as opposed to the control group improved in the post-test and continued to improve significantly in the delayed post-test. Following analysis of the participant interviews four themes emerged. Autonomous learning and self-monitoring were the dominant themes followed by engagement and motivation, self-confidence and self-esteem, and peer learning and social interaction in decreasing order of prevalence. Combining learning with play while participants navigated within the digital learning environment proved to be an effective method of teaching young EFL learners to spell. The results of this intervention contribute to recent research in the post-COVID-19 era and mirror OECD goals for the 21st century which have highlighted the need for effective digital platforms that promote independent learning while maximizing student engagement.
Association between serum free fatty acids and gestational diabetes mellitus
Authors: Li Danyang , Du Haoyi , Wu Na
Published: November 2024

Background

Pregnant women with gestational diabetes mellitus (GDM) are at an increased risk of adverse pregnancy outcomes (APO). Early understanding of risk factors affecting these outcomes may facilitate preventive interventions for women at high risk. Blood samples from GDM and control pregnant women were collected for Free fatty acid (FFA) profiling to determine the relationship with the occurrence of APO in GDM pregnant women.

Methods

The study comprised 144 women diagnosed with GDM and 52 normal control pregnancy (NC). Venous fasting serum samples were collected during the second trimester. The serum FFA levels were detected by liquid chromatography-mass spectrometry (LC-MS). The primary outcome consisted of serious maternal and neonatal adverse events ( hypertensive disorder complicating pregnancy (HDCP), emergency cesarean section, large for gestational age (LGA), small for gestational age (SGA), macrosomia, low birth weight (LBW), preterm birth, and stillbirth). The association of metrics with outcomes was assessed, and receiver operating characteristic (ROC) curve analysis was employed to evaluate clinical utility.

Results

Differences in fatty acid profiles were observed between GDM patients and controls. Stearic acid (C18:0) levels differed between the normal pregnancy outcome (NPO) and APO groups, potentially correlating with fetal sex. Logistic regression models indicated that moderate and high levels of C18:0 were negatively associated with APO relative to the NPO group. ROC analysis demonstrated that C18:0 had a certain predictive ability for APO, and predictive efficiency was enhanced when combined with general clinical data.

Conclusion

The level of C18:0 was associated with the occurrence of APO in pregnant women with GDM and exhibited a certain predictive value. When C18:0 was combined with general clinical data, the predictive power for APO was improved.
Hyperlipidemia and apolipoprotein E are associated with intraocular pressure of thyroid-associated ophthalmopathy in a Chinese population: a cross-sectional study
Authors: Chen Yu , Qi Xin , Wang Jingya , Xu Huayang , Sun Yushi , Wang Ling , Zhou Xingchen , He Mingqian , Zhao Jiarui , Zhang Jinbo , He Hairong , Guo Hui , Shi Bingyin , Wang Yue , Zhang Meng
Published: November 2024

Objective

This study aimed to explore the clinical characteristics of thyroid-associated ophthalmopathy (TAO) with hyperlipidemia and to identify the key lipid indicator.

Methods

Patients with TAO were recruited to this study and divided into two groups based on the presence of hyperlipidemia. TAO patients with hyperlipidemia were further classified based on the type of hyperlipidemia. Basic and clinical information of the patients were collected, and comparisons between groups were carried out. Correlation analyses, regression analyses, and stratified analysis were performed to assess the correlations and relationship of the serum lipids with the ophthalmic indicators.

Results

A total of 273 patients with TAO were recruited, including 158 patients with hyperlipidemia and 115 patients without hyperlipidemia. Patients with hyperlipidemia, especially those with mixed hyperlipidemia, exhibited high intraocular pressure (IOP). Spearman’s correlation analysis and partial correlation analysis showed that apolipoprotein E (ApoE) was positively related to IOP levels after controlling for confounding factors, including age, gender, BMI, smoking history, triiodothyronine (T3), and thyrotropin (thyroid-stimulating hormone, TSH). Moreover, multiple linear regression obtained a regression equation including ApoE, gender, age, and BMI and showed that elevated ApoE levels were associated with elevated IOP [β = 0.072, 95% confidence interval (CI) = 0.037–0.155, p = 0.030]. Stratified analysis highlighted the impact of ApoE on IOP in younger patients (≤48 years), female patients, patients with normal BMI (<24 kg/m2), and patients with a shorter course of ophthalmopathy (≤6 months).

Conclusion

Overall, higher IOP levels were observed in patients with hyperlipidemia, especially those with mixed hyperlipidemia. Notably, a higher ApoE was identified as an independent risk factor for higher IOP. This finding confirmed the close relationship between TAO and lipid metabolism and provides a new insight into the pathogenesis research and the long-term management of TAO.
Magnetic nanoparticles for eliminating endocrine-disrupting compounds in water treatment – a quantitative systematic analysis
Authors: Guimarães Juliana , Taveira Igor , Anacleto Thuane Mendes , Enrich-Prast Alex , Abreu Fernanda
Published: November 2024

Endocrine-disrupting compounds (EDCs) are a group of emergent pollutants that affect the endocrine system. EDCs can cause harm to humans and animals and unbalance the aquatic ecosystem even at low concentrations. Several methods are applied to remove EDCs, and recently, magnetic nanoparticles (MNPs), a low-cost and magnetically-responsive approach, have been introduced as modern innovative tools for this purpose. Thus, we conducted a systematic analysis to determine the characteristics of MNPs required to optimize EDCs removal in water/waste treatments. Most studies used magnetite (Fe3O4) nanoparticles, a low-cost and easily acquired material, followed by other metallic oxides nanoparticles (MOPs) like zinc, copper, and nickel oxides. Short adsorption time (0.5–15 min) and small MNP (10–80 nm) were the most prominent and successful in EDCs recovery approaches. After analyzing data published in the last decade, results showed that, in general, processes that apply lower concentrations of MNP seem to achieve a significant higher removal rate. For instance, MNPs formulations are able to successfully remove >90% of bisphenols and phthalates. However, depending on the EDCs type, the concentration of MNPs have to be adjusted to increase EDCs recovery rates, possibly due to their different chemical compositions and properties. Our results indicate that MNPs are an efficient, with potential to be a relatively cheap way to extract or eliminate EDCs from wastewater and should be considered an innovative tool to be included in waste treatment protocols.
Spatio-temporal variations of vegetation cover and its influencing factors in highland lake basin
Authors: Liu Zhoujiang , Hu Wenxian , Ma Liming , Huang Xiaoxia
Published: November 2024

Exploring dynamic vegetation changes and identifying the factors driving these changes are important for evaluating global ecosystem processes. Based on the pixel binary model, coefficient of variation, Theil-Sen median trend analysis, geographic detector, and Pearson correlation coefficient, this study analyzed vegetation cover variations and the factors influencing these changes in the Erhai Lake Basin, one of the most important plateau lakes in China. Vegetation cover exhibited a continuously increasing trend, with the proportion of high vegetation coverage consistently ranking first. Land cover is an effective explanatory factor for vegetation cover, and FVC shows obvious variation rules associated with elevation, land cover, population, and landform. It is important to highlight that the combination of two factors influences vegetation dynamics more significantly than one factor alone, with the interaction between land cover type and nightlight illumination being more powerful. These results enhance our understanding of the complex processes of vegetation cover variation in plateau lake catchments and offer a scientific reference for improving the spatial layout of vegetation in fragile ecosystems.
The use of volatile organic compounds in preventing and managing invasive plant pests and pathogens
Authors: Favaro Riccardo , Berka Miroslav , Pettersson Martin , Thöming Gunda , Arce Carla C. M. , Inácio Maria L. , Turlings Ted C. J. , Faria Jorge M. S. , Jung Thomas , Bazin Damien , Pozzebon Alberto , Angeli Sergio , Cappellin Luca
Published: November 2024

Invasive pests and plant pathogens pose a significant threat to ecosystems and economies worldwide, prompting the need of anticipatory strategies. Preventing their introduction by detection at the ports of entry has been proven extremely difficult. This review explores the potential of biogenic volatile detection as a reliable preventive solution. It underscores the importance of early detection and rapid response as integral components of effective invasive pest management, and it discusses the limitations of current control measures and the increasing globalization that facilitates the spread of pests and pathogens. Through a synthesis of existing literature, this review analyzes the Volatile Organic Compound (VOC) emissions in five invasive model species: three insects, Halyomorpha halys, Spodoptera frugiperda, Helicoverpa armigera, a nematode, Bursaphelenchus xylophilus, and an oomycete, Phytophthora ramorum. The review focuses on the specific volatiles, released by both the invasive organisms and the infested host plants. If available, the volatiles emitted from similar species were considered for comparison. Ultimately, this review highlights specific pest volatile and shared Herbivore Induced Plant Volatiles (HIPVs) as a reliable and innovative solution in pest detection. If possible, candidate compounds are provided, whilst the lack of some emphasizes the urge of expanding the information available.
Research hotspots and trends for Duchenne muscular dystrophy: a machine learning bibliometric analysis from 2004 to 2023
Authors: Fang Pingping , Han Jingzhe , An Di , Bu Yi , Ji Guang , Liu Mingjuan , Deng Jinliang , Guo Moran , Han Xu , Wu Hongran , Ma Shaojuan , Song Xueqin
Published: November 2024

Aims

The aim of this study was to conduct a bibliometric analysis of the relevant literature on Duchenne muscular dystrophy (DMD) to ascertain its current status, identify key areas of research and demonstrate the evolution of the field.

Methods

The analysis sourced documents from the Science Citation Index Expanded in the Web of Science core collection, utilizing CiteSpace software and an online bibliometric platform to analyze collaborative networks among authors, institutions and countries, and to map out the research landscape through journal and reference evaluations. Keyword analyses, including clustering and emergent term identification, were conducted, alongside the development of knowledge maps.

Results

The study included 9,277 documents, indicating a rising publication trend in the field. The Institut National de la Santé et de la Recherche Médicale emerged as the top publishing institution, with Francesco Muntoni as the most prolific author. The United States dominated in publication output, showcasing significant leadership. The keyword analysis highlighted 786 key emergent terms, primarily focusing on the mechanisms, diagnostics and treatment approaches in DMD.

Conclusion

The field of DMD research is experiencing robust growth, drawing keen interest globally. A thorough analysis of current research and trends is essential for advancing knowledge and therapeutic strategies in this domain.
The genome survey of male and female Hapalogenys analis
Authors: Liu Kai , Qu Yin-Quan , Shi Hui-Lai , Ping Hong-Ling , Gao Tian-Xiang
Published: November 2024

Effectively assessing the basic genomic information of a species is fundamental for conducting molecular research and provides a foundation for constructing whole-genome maps. Hapalogenys analis is a temperate and tropical nearshore marine fish in China’s coastal waters, with significant economic value and high aquaculture potential. However, the genomic differences between male and female individuals of this species are not yet apparent. This study conducted whole-genome survey analyses on male and female H. analis to provide basic genomic information. According to K-mer analysis, the genome sizes of female and male fish were 436.24 Mb and 493.21 Mb. The heterozygosity rates were 0.58% for females and 0.23% for males. The proportion of repetitive sequences of female and male fish were 42.95% and 51.20%. The GC content of the genomes was 43.30% for female and 43.20% for male. The sizes of the assembled genomes were 589.18 Mb for female and 592.02 Mb for male, with N50 lengths of 3,135 bp and 3,041 bp, respectively. SSR screening results showed that 959,447 and 894,158 SSR sequences were detected in the genomes of female and male, respectively. The lengths of the assembled mitochondrial genome sequences were 19,755 bp for female and 19,754 bp for male, each containing 38 genes. Among these mitochondrial sequences, 13 protein-coding genes were identified, including 7 NADH dehydrogenase, 3 cytochrome c oxidase, 1 cytochrome b, and 2 ATP synthase genes. Both sequences contained 23 tRNA genes and 2 rRNA genes. This study provides a theoretical basis for constructing a high-quality whole genome of H. analis and valuable data for subsequent molecular breeding research.
Estimating total microplastic loads to the marine environment as a result of ship biofouling in-water cleaning
Authors: Soon Zhi Yang , Tamburri Mario N. , Kim Taekhyun , Kim Moonkoo
Published: November 2024

The widespread use of polymers across various industries has led to significant microplastic pollution in marine environments, with millions of tons of microplastics being released annually. This study examines the contribution of coatings particles released from commercial ships, to marine microplastic pollution. Key sources of these microplastics include the weathering and abrasion of coatings during ship maintenance activities. Marine coatings, which are rich in polymers such as polyurethanes and epoxies, are released into the ocean through processes like normal wear and tear, damage, in-water cleaning, and removal of old paint layers. Our research indicates that the global shipping sector could be a substantial contributor to microplastic pollution, potentially releasing thousands of tons annually. Predictive modeling identifies bulk carriers as the largest contributors, followed by tankers, containerships, and cargo vessels. This study also finds that manual biofouling cleaning by divers generates more microplastics than cleaning using mechanized in-water cleaning (IWC) systems with debris capture. Mitigation strategies, such as alternative cleaning methods and improved waste capture and processing, are proposed, but their effectiveness remains uncertain due to implementation challenges. A multidisciplinary approach and coordinated global efforts are essential to develop effective strategies for reducing microplastic pollution from ship coatings and protecting marine ecosystems.
The past, present, and future of endoscopic management for biliary strictures: technological innovations and stent advancements
Authors: Ni Dong-jin , Yang Qi-fan , Nie Lu , Xu Jian , He Si-zhe , Yao Jun
Published: November 2024

Biliary stricture can be induced by intrinsic narrowing and extrinsic compression, with the majority of cases being malignant. Clinically, distinguishing between benign and malignant biliary strictures remains a considerable challenge, and the ongoing disagreement over the optimal choice of biliary stents significantly influences treatment strategies and impacts patients’ survival and prognosis. The utilization and advancement of endoscopic techniques have heightened the diagnostic sensitivity for biliary strictures. Concurrently, innovative technologies such as endoscopic ultrasound and magnetic compression anastomosis emerge as viable alternatives when endoscopic retrograde cholangiopancreatography (ERCP) is not an option, providing fresh insights for the clinical management of these patients. Traditional plastic and metal stents, characterized by their complex application and limited scope, have been unable to fully satisfy clinical needs. The introduction of novel stent varieties has notably improved this scenario, marking a considerable progression towards precision medicine. However, the clinical validation of the diverse stent materials available is incomplete. Hence, a thorough discussion on the present state and evolving trends of biliary stents is warranted.
Capsule endoscopy aspiration and respiratory physician’s treatment insights: a case report and literature review
Authors: Zhou Yinxue , Wang Hongmei , Zhuang Min , Liu Hua , Qi Lijie , Zhang Lingyun , Sun Jiaxing
Published: November 2024

Background

Capsule endoscopy (CE) is widely used for intestinal examination; however, capsule aspiration into the airway is a serious complication that requires urgent intervention. We present a management case report and review 39 cases from 2003 to 2023, providing insights into the prevention and treatment of capsule aspiration.

Case presentation

A 69-year-old man with chronic bronchitis and emphysema presented with 7 months of intermittent melena. After swallowing a capsule endoscope (PillCam™ SB 3), he had a brief cough and chest tightness. Imaging confirmed aspiration in the right intermediate bronchus, and non-invasive removal procedures were unsuccessful.

Methods

Real-time imaging confirmed the lodged capsule. Non-invasive methods, such as coughing and chest percussion, were unsuccessful. Therefore, flexible bronchoscopy was performed under general anesthesia to retrieve the capsule using a snare, which was then placed into the duodenum using a gastroscope.

Results

The capsule was successfully retrieved, and the patient recovered well, completing the endoscopy without further issues.

Conclusion

Our case study and literature review highlight the need for careful attention to high-risk groups in CE, including the elderly and individuals with neurological or swallowing difficulties. A thorough history review and real-time monitoring are essential for preventing complications. Bronchoscopy is preferred for CE retrieval due to its advantages. Manufacturers are urged to improve CE safety, with respiratory physicians helping internists in managing this potentially life-threatening complication.
Unraveling the cGAS/STING signaling mechanism: impact on glycerolipid metabolism and diseases
Authors: Su Jie , Cheng Fuyu , Yuan Wei
Published: November 2024

The cyclic GMP-AMP synthase (cGAS) and its downstream effector, the stimulator of interferon genes (STING), are crucial components of the innate immune response, traditionally recognized for their role in detecting cytosolic DNA from pathogens and damaged host cells. However, recent research indicates that the cGAS-STING pathway also significantly impacts metabolic processes, particularly glycerolipid metabolism. Glycerolipids are essential for energy storage and cellular membrane integrity, and their dysregulation is linked to metabolic disorders such as obesity, insulin resistance, and non-alcoholic fatty liver disease (NAFLD). Both cGAS and STING are expressed in various metabolic tissues, suggesting a potential role in lipid homeostasis. Chronic activation of the cGAS-STING pathway may promote inflammatory states that exacerbate insulin resistance and lipid accumulation, forming a feedback loop of metabolic dysfunction. This review explores the emerging relationship between cGAS/STING signaling and glycerolipid metabolism, discussing the mechanisms through which this pathway influences lipid regulation and the potential for therapeutic interventions. By integrating insights from immunology and metabolism, we aim to provide a comprehensive understanding of how the cGAS-STING axis may serve as a novel target for addressing metabolic disorders and enhancing metabolic health outcomes.
Evaluation of Streptomyces sporoverrucosus B-1662 for biological control of red pepper anthracnose and apple bitter rot diseases in Korea
Authors: Kim DaYoung , Kim Jungyeon , Lee Younmi , Balaraju Kotnala , Hwang Ye-Ji , Lee Mi-Hwa , Cheon Wonsu , Mun Hye Yeon , Lee Chang Soo , Jeon Yongho
Published: November 2024

Fungi are the prominent phytopathogens that have significant impact on the productivity of agriculture worldwide. Streptomyces species have been extensively studied for the production of various bioactive metabolites. These metabolites have been used as biocontrol agents for the management of diseases caused by phytopathogenic fungi. The purpose of this investigation is to assess the efficacy of Streptomyces sporoverrucosus B-1662, an antagonistic agent in the control of red pepper anthracnose caused by Colletotrichum acutatum KACC 42403 and apple anthracnose caused by Colletotrichum siamense CGCP6 (GYUN-10348). On the basis of the morphological, and molecular characterization using 16S rRNA, the strain B-1662 was determined to be S. sporoverrucosus. The strain B-1662 exhibited antagonistic activity against seven fungal phytopathogens, including C. acutatum KACC 42403 and C. siamense CGCP6. The culture filtrates (CF) from B-1662 showed antifungal activity against all seven fungal pathogens with greater inhibition rate (%) in comparison with a control. The bacterial suspensions of B-1662 showed an excellent biological control effect on the red pepper anthracnose and apple bitter rot using an in planta assay. The anthracnose disease rate (%) was controlled by over 90% with B-1662 cell suspensions at 105 to 107 CFU/mL. Compared to a control, the strain B-1662 played a more effective role in controlling the anthracnose disease in field conditions in both years 2022 and 2023. From the effective solvent fractions, the effect compound (dibutoxybutane) has been isolated exhibiting with antifungal effect. The genetic base underlying the biocontrol traits of B-1662 was characterized using the whole-genome sequence of B-1662, which was compared with closely related strains. Consequently, these results collectively suggest that S. sporoverrucosus B-1662 can aid in the management of red-pepper anthracnose.
Correlation between triglyceride-glucose index and early neurological deterioration in patients with acute mild ischemic stroke
Authors: Liu Yang , Wang Zhiye , Zhang Zuonian , Lu Zhaomin , Zhang Lihua , Ding Wei , Fang Kai , Pan Xijin , Ni Mengyuan , Liu Junjun
Published: November 2024

Objective

The Triglyceride-glucose Index (TyG) index is a dependable metric for assessing the degree of insulin resistance, serving as a standalone predictor of ischemic stroke risk, but its precise relationship with early neurological deterioration (END) remains incompletely expounded within the context of acute mild ischemic stroke patients. This research is to examine the correlation of the TyG index with END among patients experiencing acute mild ischemic stroke in China.

Methods

This retrospective analysis was conducted to systematically gather data regarding patients experiencing their maiden episode of acute mild ischemic stroke and hospitalized at the Neurology Department of Nanjing Meishan Hospital, located in Nanjing, Jiangsu Province, China, over the period extending from January 2020 to December 2022. The severity of stroke was determined through the utilization of the National Institutes of Health Stroke Scale (NIHSS) scores upon their admission. Demographic characteristics were collected, and measurements of fasting blood glucose, blood lipids, and glycosylated hemoglobin Alc levels were taken. END was defined as a one-point rise in the motor item function score on the NIHSS or a two-point increase in the overall score during the initial 72 h of hospitalization. For evaluating the correlation of the TyG index with END, a multivariate logistic regression analysis was carried out. To investigate whether there is a nonlinear relationship between the TyG index and END, smoothed curves were utilized.

Results

The study included 402 patients diagnosed with acute mild ischemic stroke, with a mean age of 66.15 ± 10.04 years. Within this population, 205 were males (51.00%) and 197 were females (49.00%). Among these patients, 107 (26.62%) experienced END within 72 h of admission. Patients who developed END showed higher levels of the TyG index in comparison to those who remained stable (9.18 ± 0.46 vs. 8.87 ± 0.46, p < 0.001). In a comprehensive multivariate logistic regression analysis, the TyG index positively correlates with END (OR = 3.63, 95% CI: 1.75–7.54, p = 0.001). Furthermore, individuals in the fourth TyG index quartile exhibited a 2.36-fold heightened risk of END compared to those in the first quartile (95% CI: 1.38–8.19, p = 0.008). TyG index has a linear correlation with END in the generalized additive model (Log likelihood ratio test, p = 0.525).

Conclusion

Our findings demonstrate that TyG index has a significant, independent, and positive correlation with END in Chinese individuals diagnosed with acute mild ischemic stroke. This underscores the TyG index’s potential usefulness as a valuable risk stratification tool for stroke patients.
Ocular findings in neurosyphilis: a retrospective study from 2012 to 2022
Authors: Cheng Huanhuan , Zhu Haocheng , Shen Gang , Cheng Yaqi , Gong Jiao , Deng Juan
Published: November 2024

Purpose

This study aimed to investigate ocular manifestations in patients with neurosyphilis and their association with general indexes.

Methods

This retrospective study was conducted among patients who were hospitalized for neurosyphilis from 2012 to 2022. Clinical manifestations, laboratory tests, brain MRI, and ocular examinations were reviewed and analyzed.

Results

A total of 106 neurosyphilis patients were included, of which 10 patients presented with ocular signs as their initial symptoms. The most common main complaint was reduced vision (6/10), followed by diplopia (2/10) and ptosis (2/10). The causes of vision loss included optic neuropathy (4/6) and posterior uveitis (2/6), with all six patients showing optic nerve involvement. A total of 29 (27.4%) patients exhibited pupil abnormalities. A lumbar puncture was performed on all 106 patients, and abnormal cerebrospinal fluid (CSF) findings were revealed in 101 (95.3%) patients, indicating central nervous involvement. The median white blood cell count in the CSF of the patients with pupil abnormalities was significantly higher than that of those without pupil abnormalities (14.0 vs. 6.0 cells/μl, p = 0.037). In addition, the patients with abnormal pupillary light reflex (PLR) were more likely to have multiple brain lesions compared to those with normal PLR (77.3% vs. 29.2%, p < 0.001).

Conclusion

Optic nerve involvement is the main cause of vision loss in neurosyphilis. Patients with optic neuropathy or posterior uveitis should undergo prompt diagnostic evaluation for syphilis. Pupil abnormalities can serve as indicators of more severe CSF and MRI findings.
Herbal placebo response in clinical trials on irritable bowel syndrome: a systematic review and meta-analysis
Authors: Huang Kaiyue , Lv Mi , Zheng Ting , Wang Fengyun , Tang Xudong , Lv Lin
Published: November 2024

Aim of the study

To systematically evaluate the herbal placebo response in randomized controlled trials (RCTs) of herbal medicine on irritable bowel syndrome (IBS).

Materials and methods

We searched for RCTs with herbal placebo groups for IBS in PubMed, EMBASE, the Cochrane Library, the China National Knowledge Infrastructure (CNKI), the Wan Fang database and Sinomed database from 31 January 1994 to November 2023, and the quality of the literature was evaluated by the Cochrane risk of bias assessment criteria. The primary outcome indicators were response rate, abdominal pain and stool improvement rate, which were analyzed by single-group rate meta-analysis. Secondary outcomes were analyzed in subgroups based on diagnostic criteria, duration of treatment, subtype, research locations, placebo form, and presence of herbal ingredients to look for factors affecting respond rate.

Results

The study included 24 papers, involving a total of 2,596 patients. Of these, 1151 IBS patients were treated with the herbal placebo. The placebo response rate in IBS patients in the herbal placebo group was 37% (P < 0.01,I2 = 75%). A total of 287 patients in five studies were given the herbal placebo, and the improvement rate of abdominal pain was 29% (P = 0.83, I2 = 0%). Four studies enrolled a total of 212 patients with IBS who received herbal placebo, and the stool improvement rate was 46% (P = 0.02 < 0.05, I2 = 71%). The research locations and treatment duration were sources of heterogeneity (P < 0.05).

Conclusion

There is a significant herbal placebo response in patients with IBS. Different research locations and treatment durations are major sources of heterogeneity that may affect IBS patient response rates. The addition of a low dose of herbal ingredients when simulating an herbal placebo does not exaggerate the therapeutic effect of the placebo. There is a lack of uniformity and standardization in the preparation and evaluation of herbal placebos.
Network pharmacology, experimental validation and pharmacokinetics integrated strategy to reveal pharmacological mechanism of goutengsan on methamphetamine dependence
Authors: Li Han-Cheng , Li Jie-Yu , Wang Xing-Chen , Zeng Ming , Wu Yang-Kai , Chen Yi-Ling , Kong Cai-Hua , Chen Ke-Lin , Wu Jie-Ru , Mo Zhi-Xian , Zhang Jia-Xuan , Liu Chang-Shun
Published: November 2024

Background

Goutengsan (GTS) is a traditional Chinese medicine formula that can improve multiple nervous system diseases, such as methamphetamine (MA) dependence. However, the mechanism how GTS treats MA dependence remains unclear. This study was aimed to investigate the action mechanism of GTS on MA dependence using network pharmacology, in vivo/in vitro experimental validation, pharmacokinetics, and tissue distribution in the brain.

Materials and Methods

The bioactive ingredients from GTS and possible targeted genes for treating MA dependence were predicted using network pharmacology. The binding of key components of GTS to the predicted proteins was studied using molecular docking, and the key components were verified by HPLC. The effects of GTS on an MA-induced model in rats and SH-SY5Y cells were studied. The regulatory effects of GTS on the expressions of predicted MAPK pathway-related proteins in rat brain tissues and SH-SY5Y cells were validated. Furthermore, the plasma exposure and brain tissue distribution of GTS ingredients for MA dependence treatment and MAPK pathway regulation were studied in mice.

Results

Network pharmacology screened 53 active ingredients, and 287 potential targets of GTS, and showed the MAPK pathway was among the most relevant pathways. Molecular docking showed that key active ingredients (e.g., 6-gingerol, liquiritin and rhynchophylline) bound strongly with MAPK core targets, such as MAPK3, and MAPK8. Five compounds of GTS were detected by HPLC, including 6-gingerol, chlorogenic acid, liquiritin, 5-o-methylviscumaboloside and hesperidin. GTS had a therapeutic effect on MA-dependent rats, and reduced hippocampal CA1 damage and relative expressions of p-MAPK3/MAPK3, p-MAPK8/MAPK8 in brain tissues induced by MA. GTS counteracted aberrant alterations in cAMP, 5-TH and cellular morphology induced by MA induction and exerts therapeutic effects on MA-induced SH-SY5Y cell models. GTS also can antagonize the high expressions of MAPK-related proteins in MA-induced SH-SY5Y cells. Pharmacokinetic experiment revealed the four ingredients of GTS (e.g., chlorogenic acid, 5-o-methylviscumaboloside, hesperidin and rhynchophylline) were exposed in the plasma and brain, which demonstrates its pharmacological effect on MA dependence.

Conclusion

GTS treats MA dependence by regulating the MAPK pathway via multiple bioactive ingredients. The network pharmacology, experimental validation and pharmacokinetics integrated strategy is efficient in discovering the key pharmacological mechanism of herbal formulae.
CsWAK12, a novel cell wall-associated receptor kinase gene from Camellia sinensis, promotes growth but reduces cold tolerance in Arabidopsis
Authors: Wu Qiong , Jiao Xiaoyu , Liu Dandan , Sun Minghui , Tong Wei , Ruan Xu , Wang Leigang , Ding Yong , Zhang Zhengzhu , Wang Wenjie , Xia Enhua
Published: November 2024

Cold significantly impacts the growth and development of tea plants, thereby affecting their economic value. Receptor-like kinases (RLKs) are thought to play a pivotal role in signaling the plant's response to cold and regulating cold tolerance. Among the RLK subfamilies, wall-associated receptor-like kinases (WAKs) have been investigated across various plant species and have been shown to regulate cell growth and stress responses. However, the function of WAK genes in response to cold stress in tea has yet to be studied. In a previous investigation, we identified the WAK gene family from Camellia sinensis and isolated a specific WAK gene, CsWAK12, which is induced by abiotic stresses. Here, we demonstrate that CsWAK12 is involved in the regulation of cold tolerance in tea plants. CsWAK12 was rapidly induced by cold, peaking at 3 hours after treatment at 4°C (10-fold increase). Heterologous overexpression of CsWAK12 (35S:CsWAK12) in Arabidopsis promoted plant growth by enhancing root length and seed size under normal conditions, although it reduced cold resistance compared to the wild type. Under cold stress, the transgenic plants exhibited a lower survival rate and significantly altered levels of superoxide dismutase (SOD) activity and malondialdehyde (MDA) content compared to the wild type (WT). Furthermore, the expression of C-repeat/dehydration-responsive element binding factor (CBF) genes was diminished in CsWAK12-overexpressing transgenic Arabidopsis plants following cold treatment. Transcriptome analysis revealed that genes associated with the CBF pathway, such as transcription factor genes (ERF53, ERF54, and DREB2A) were markedly reduced in the overexpression line. These data suggest that CsWAK12 acts as a negative regulator, reducing the cold tolerance of transgenic Arabidopsis by mediating the CBF pathway. Therefore, CsWAK12 may serve as a candidate gene for the molecular breeding of cold resistance in tea plants.
Elemental profiling and genome-wide association studies reveal genomic variants modulating ionomic composition in Populus trichocarpa leaves
Authors: Ployet Raphael , Feng Kai , Zhang Jin , Baxter Ivan , Glasgow David C. , Andrews Hunter B. , Rodriguez Miguel , Chen Jin-Gui , Tuskan Gerald A. , Tschaplinski Timothy J. , Weston David J. , Martin Madhavi Z. , Muchero Wellington
Published: November 2024

The ionome represents elemental composition in plant tissues and can be an indicator of nutrient status as well as overall plant performance. Thus, identifying genetic determinants governing elemental uptake and storage is an important goal for breeding and engineering biomass feedstocks with improved performance. In this study, we coupled high-throughput ionome characterization of leaf tissues with high-resolution genome-wide association studies (GWAS) to uncover genetic loci that modulate ionomic composition in leaves of poplar (Populus trichocarpa). Significant agreement was observed across the three ionomic profiling platforms tested: inductively coupled plasma-mass spectrometry (ICP-MS), neutron activation analysis (NAA) and laser-induced breakdown spectroscopy (LIBS). Relative quantification of 20 elements using ICP-MS across a population of 584 genotypes, revealed larger variation in micro-nutrients and trace elements content than for macro-nutrients across genotypes. The GWAS performed using a set of high-density (>8.2 million) single nucleotide polymorphisms, identified over 600 loci significantly associated with variations in these mineral elements, pointing to numerous uncharacterized candidate genes. A significant enrichment for genes related to ion homeostasis and transport was observed, including several members of the cation-proton antiporters (CPA) family and MATE efflux transporters, previously reported to be critical for plant growth and fitness in other species. Our results also included a polymorphic copy of the high-affinity molybdenum transporter MOT1 found directly associated to molybdenum content. For the first time in a perennial plant, our results provide evidence of genetic control of mineral content in a model tree species.
Testcross performance and combining ability of intermediate maturing drought tolerant maize inbred lines in Sub-Saharan Africa
Authors: Manigben Kulai Amadu , Beyene Yoseph , Chaikam Vijay , Tongoona Pangirayi B. , Danquah Eric Y. , Ifie Beatrice E. , Aleri Isaiah , Chavangi Andrew , Prasanna Boddupalli M. , Gowda Manje
Published: November 2024

Drought is a major constraint on maize (Zea mays L.) production and productivity in Sub-Saharan Africa (SSA). The increase in frequency and severity of drought, driven by climate change, is expected to worsen in the future. These occurrences are likely to adversely affect maize production and productivity, threatening the economic and social stability of millions of smallholder farmers. Understanding the genetics of hybrid performance under drought stress is crucial for designing breeding strategies to develop high-yielding hybrids. This study aimed to (i) evaluate the performance of three-way cross hybrids developed from elite inbred lines, including several drought-tolerant lines, using a line-by-tester mating design, and (ii) estimate the general combining ability (GCA) and specific combining ability (SCA) effects of the tropical maize inbred lines under managed drought and optimum conditions. A total of 265 maize inbred lines from the CIMMYT global maize breeding program were used as parents and crossed to six single cross testers to generate 795 testcross hybrids. These hybrids, along with six commercial hybrids as a check, were evaluated under managed drought and optimum conditions. Significant (p < 0.001) variations were observed among genotypes and genotypes-by-environment interactions (GEIs) for grain yield and other traits. There was a preponderance of GCA variance (lines and tester) over SCA variance, indicating that additive effects were more important in determining grain yield and other key traits under both managed drought and optimum conditions. Ten inbred lines (S2_8, S10_1, S6_4, S10_14, S2_14, S10_15, S8_7, S2_3, S8_15, and S13_5) with desirable GCA effects for grain yield and other traits were identified. Fourteen testcross hybrids were identified with high grain yield and desirable agronomic traits under both drought and optimum conditions. The identified lines and hybrids are useful sources to be used in breeding and deploying as stress-tolerant hybrids. High correlations observed between observed and GCA-predicted hybrid performance suggest the possibility to evaluate more hybrids with fixed resources. The study demonstrates that it is feasible to obtain high-yielding and drought-tolerant lines and hybrids. These testcross hybrids should undergo rigorous on-farm trials to ensure consistent performance before commercialization and release. Deploying these hybrids could help in mitigating the effects of drought stress in SSA and contribute to improved maize productivity in the region.
Association mapping for water use efficiency in soybean identifies previously reported and novel loci and permits genomic prediction
Authors: Chamarthi Siva K. , Purcell Larry C. , Fritschi Felix B. , Ray Jeffery D. , Smith James R. , Kaler Avjinder S. , King C. Andy , Gillman Jason D.
Published: November 2024

Soybean is a major legume crop cultivated globally due to the high quality and quantity of its seed protein and oil. However, drought stress is the most significant factor that decreases soybean yield, and more than 90% of US soybean acreage is dependent on rainfall. Water use efficiency (WUE) is positively correlated with the carbon isotopic ratio 13C/12C (C13 ratio) and selecting soybean varieties for high C13 ratio may enhance WUE and help improve tolerance to drought. Our study objective was to identify genetic loci associated with C13 ratio using a diverse set of 205 soybean maturity group IV accessions, and to examine the genomic prediction accuracy of C13 ratio across a range of environments. An accession panel was grown and assessed across seven distinct combinations of site, year and treatment, with five site-years under irrigation and two site-years under drought stress. Genome-wide association mapping (GWAM) analysis identified 103 significant single nucleotide polymorphisms (SNPs) representing 93 loci associated with alterations to C13 ratio. Out of these 93 loci, 62 loci coincided with previous studies, and 31 were novel. Regions tagged by 96 significant SNPs overlapped with 550 candidate genes involved in plant stress responses. These confirmed genomic loci could serve as a valuable resource for marker-assisted selection to enhance WUE and drought tolerance in soybean. This study also demonstrated that genomic prediction can accurately predict C13 ratio across different genotypes and environments and by examining only significant SNPs identified by GWAM analysis, higher prediction accuracies (P ≤ 0.05; 0.51 ≤ r ≤ 0.65) were observed. We generated genomic estimated breeding values for each genotype in the entire USDA-GRIN germplasm collection for which there was marker data. This information was used to identify the top ten extreme genotypes for each soybean maturity group, which could serve as valuable genetic and physiological resources for future breeding and physiological studies.
Warming altered the effect of cold stratification on the germination of Spartina alterniflora across climatic zones in its invasive range
Authors: Wu Fujia , Chen Xincong , Guo Yangping , Liu Wenwen , Zhang Yihui
Published: November 2024

Introduction

Cold stratification has a pronounced influence on seed germination, climate change is altering cold stratification regimes across climatic zones. Therefore, it is urgent to explore how seed germination from different geographic provenances responds to these changes. The invasive plant Spartina alterniflora spans three climatic zones along the Chinese coast, such distribution provides a natural temperature gradient to explore how warming alters the effects of cold stratification on germination.

Methods

Spartina alterniflora seeds were collected from nine locations across three climatic zones in China from September to November in 2021. Seeds were planted in three common gardens with three latitude gradients of 21 °N, 28 °N, and 38 °N, after 0-month and 4-month cold stratification at 4 °C in November 2021 and March 2022, respectively. Each common garden simulated the natural temperature conditions and shield the plants from rain.

Results

Results showed that cold stratification led to explosive germination and rapidly reaching a plateau, shortened the germination time and improved the final germination rate. These effects were magnified from the high-latitude garden to the low-latitude one (i.e., warming). And the interactive effect of cold stratification and warming varied among provenances. For the subtropical and temperate provenances, the improvement in germination rate induced by cold stratification gradually increased from high-latitude garden to low-latitude one, while for tropical provenances, this difference progressively decreased. Discussion: Thus, our results indicated that subtropical and temperate provenances may migrate northward for adequate low temperatures to ensure high germination rate, because cold stratification can alleviate the negative impacts of warming on germination. For the tropical provenances, warming also suppressed the advantage that cold stratification provides in enhancing the germination rate, which may hinder their further spread southward. Our study contributes to understanding the responses of vegetation germination and recruitment across different climatic zones under global warming, providing insights for the distribution of cosmopolitan species and the management of exotic species.
The impact of early life experiences on inhibitory control and working memory
Authors: Dydenkova Eva , McGlone Francis , Mayorova Larisa , Nikolaeva Elena
Published: November 2024

Introduction

Adverse childhood experiences (ACEs) are a general term for a large group of nonequivalent situations that have the potential to traumatise a child. This risk factor is caused by a sensitive period of brain development, which is based on myelination, creation of synaptic connections and pruning. Dramatic environmental events during this period, such as history of institutionalisation, can disrupt optimal developmental pathways, leaving biological scars for life.

Methods

The focus of this study was to investigate the impact of institutionalisation on the development of inhibitory control and working memory in three groups of children matched for age (n = 130; 7.1 ± 2.0 years): (1) early institutionalised (n = 35; age of placement: 6.9 ± 10.6 months; duration of placement: 14.6 ± 10.4 months); (2) late institutionalised (n = 29; age of placement: 49.3 ± 30.6 months; the duration of placement: 16.0 ± 19.4 months); (3) never institutionalised (n = 66).

Results

Results showed that the early institutionalised group had the lowest scores on tests of inhibitory control (p = 0.03), working memory (p = 0.03) and retrieval-based learning (p = 0.04), while the results of the group of late institutionalised children do not differ significantly from never institutionalised.

Discussion

The existence of a sensitive period during the first 18 months of a child’s life is discussed, which determines the formation of the retrieval-based learning mechanism and of inhibiting ineffective experience, for which executive functions are responsible.