bluuebunny/crossref_metadata_2025 huggingface dataset:
Complexity and Heterogeneity Evaluation of Pore Structures in the Deep Marine Shale Reservoirs of the Longmaxi Formation, China
Authors: Zhu Boyuan , Meng Jianghui , Song Chen , Pan Renfang , Zhu Zhengping , Jin Jineng
Published: August 2023

The structural evolution and sedimentary differentiation of the Sichuan Basin in China are complex, with intricate reservoir pore structures that significantly impact shale gas production. This study examines the complexity and heterogeneity of the microscopic pore structures in the deep marine shale reservoir in the Longmaxi Formation. Pore structure characterization techniques are used to compare deep and shallow–medium marine shales, and siliceous and silty shales. The results reveal the factors influencing pore structure and their impact on exploration and development. The key points are as follows: (1) The pore structure of deep siliceous shale is the most complex due to its diverse range of pore development patterns, pore types, and sizes. (2) The box dimension of full pore size is about 1.52 for deep marine shale and 1.46 for shallow–medium shale. Organic matter (OM) content, the degree of pore development, and inorganic mineral content all correlate positively with the complexity of the pore structure in deep marine shale, which affects the formation of high-quality reservoirs. (3) Lateral heterogeneity of pore structures shows strong regional variations in the study area. Heterogeneity is more pronounced in the deep marine shale than in the medium and shallow shale formations. OM mesopores significantly influence the overall heterogeneity of the shale pore system. The deep marine shale reservoir is situated in an area with strong regional variations. The pore structure of high-quality reservoirs is more complex than those of shallow–medium marine shales, displaying notable heterogeneity. Pore structures with fractal dimension values close to that of the shallow–medium formations (box dimensions within 1.5) offer promising targets for the exploration and development of deep marine shale gas.
Estimating Permeability of Porous Media from 2D Digital Images
Authors: Lei Gang , Liu Tianle , Liao Qinzhuo , He Xupeng
Published: August 2023

Digital rock physics (DRP) has been widely used as an effective approach for estimating the permeability of porous media. However, conventional implementation of DRP requires the reconstruction of three-dimensional (3D) pore networks, which suffer from intensive memory and underlying uncertainties. Therefore, it is highly significant to develop an approach only based on two-dimensional (2D) cross-sections of parent samples without 3D reconstruction. In this study, we present a novel approach that combines the Kozeny–Carman equation with fractal theory to derive a bridge function that links 2D cross-sectional images and 3D pore structures of parent samples in flow equivalence. Using this bridge function, we predicted the physical properties of the parent samples, including the permeability, bulk porosity, tortuosity, and pore fractal dimension. To validate our model, we performed Lattice Boltzmann (LB) simulations on nine carbonate samples and compared the LB simulation results with our model’s predictions. We also compared our predicted results with available data on various porous materials, such as sandstone, glass beads, and carbonate, in the literature. Our findings demonstrate that without reconstructing 3D pore networks, our method provides a reliable estimation of sample permeability using 2D cross-sectional images. This approach not only simplifies the determination of sample permeability in heterogeneous porous media but also sheds new light on the inherent correlations between 2D cross-sectional information and 3D pore structures of parent samples. Moreover, the derived model may be conducible to a better understanding of flow in reservoirs during the extraction of unconventional onshore and offshore oil/gas.
Hydrodynamics and Wake Flow Analysis of a Floating Twin-Rotor Horizontal Axis Tidal Current Turbine in Roll Motion
Authors: Zhao Muyu , Chen Ying , Jiang Jin
Published: August 2023

The study of hydrodynamic characteristics of floating double-rotor horizontal axis tidal current turbines (FDHATTs) is of great significance for the development of tidal current energy. In this paper, the effect of roll motion on a FDHATT is investigated using the Computational Fluid Dynamics (CFD) method. The analysis was conducted in the CFD software STAR-CCM+ using the Reynolds-averaged Navier–Stokes method. The effects of different roll periods and tip speed ratios on the power coefficient and thrust coefficient of FDHATT were studied, and then the changes in the vorticity field and velocity field of the turbine wake were analyzed by two-dimensional cross-section and Q criterion. The study indicates that roll motion results in a maximum decrease of 30.76% in the average power coefficient and introduces fluctuations in the instantaneous load. Furthermore, roll motion significantly accelerates the recovery of wake velocity. Different combinations of roll periods and tip speed ratios lead to varying degrees of wake velocity recovery. In the optimal combination, at a position 12 times the rotor diameter downstream, roll motion can recover the wake velocity to 92% of the incoming flow velocity. This represents a 23% improvement compared to the case with no roll motion.
Development of Echinocandin Resistance in Candida haemulonii: An Emergent, Widespread, and Opportunistic Fungal Pathogen
Authors: Silva Laura N. , Ramos Lívia S. , Oliveira Simone S. C. , Magalhães Lucas B. , Cypriano Jefferson , Abreu Fernanda , Macedo Alexandre J. , Branquinha Marta H. , Santos André L. S.
Published: August 2023

Echinocandins, used for the prevention and treatment of invasive fungal infections, have led to a rise in breakthrough infections caused by resistant Candida species. Among these species, those belonging to the Candida haemulonii complex are rare multidrug-resistant (MDR) yeasts that are frequently misidentified but have emerged as significant healthcare-associated pathogens causing invasive infections. The objectives of this study were to investigate the evolutionary pathways of echinocandin resistance in C. haemulonii by identifying mutations in the FKS1 gene and evaluating the impact of resistance on fitness. After subjecting a MDR clinical isolate of C. haemulonii (named Ch4) to direct selection using increasing caspofungin concentrations, we successfully obtained an isolate (designated Ch4′r) that exhibited a high level of resistance, with MIC values exceeding 16 mg/L for all tested echinocandin drugs (caspofungin, micafungin, and anidulafungin). Sequence analysis revealed a specific mutation in the resistant Ch4′r strain, leading to an arginine-histidine amino acid substitution (R1354H), occurring at the G4061A position of the HS2 region of the FKS1 gene. Compared to the wild-type strain, Ch4′r exhibited significantly reduced growth proliferation, biofilm formation capability, and phagocytosis ratio, indicating a decrease in fitness. Transmission electron microscopy analysis revealed alterations in cell wall components, with a notable increase in cell wall thickness. The resistant strain also exhibited higher amounts (2.5-fold) of chitin, a cell wall-located molecule, compared to the wild-type strain. Furthermore, the resistant strain demonstrated attenuated virulence in the Galleria mellonella larval model. The evolved strain Ch4′r maintained its resistance profile in vivo since the treatment with either caspofungin or micafungin did not improve larval survival or reduce the fungal load. Taken together, our findings suggest that the acquisition of pan-echinocandin resistance occurred rapidly after drug exposure and was associated with a significant fitness cost in C. haemulonii. This is particularly concerning as echinocandins are often the first-line treatment option for MDR Candida species.
The Impact of Rural Location on Farmers’ Livelihood in the Loess Plateau: Local, Urban–Rural, and Interconnected Multi-Spatial Perspective Research
Authors: Wang Yin , Min Dian , Ye Wenli , Wu Kongsen , Yang Xinjun
Published: August 2023

With the strengthening of regional and urban–rural interactions, farmers’ livelihood activities are becoming increasingly complex, and environmental factors that influence farmers’ livelihoods have multi-spatial effects. Consequently, comprehending farmers’ livelihoods from a multi-spatial perspective is imperative. Based on surveys conducted in 65 villages and 451 households in Jia County on the Loess Plateau, China, rural locations were deconstructed into natural, traffic, and positional advantages to explore the relationships and mechanisms between the rural environment and farmers’ livelihood stability from local, urban–rural, and interconnected multi-spatial perspectives. We found that 77% of the villages achieved a moderate or high Rural Location Advantage Index (RLAI) rating; 45% still lack natural advantages and are mainly located in hilly and sandy areas because of the fragile ecological environment of the Loess Plateau. Additionally, the Livelihood Stability Index (LSI) was moderate overall, but with significant spatial heterogeneity, and 72% of farmers possess strong transition capacity and have shifted away from relying on monoculture as their primary livelihood strategy. While a certain coupling correspondence exists between the LSI and RLAI, the interaction is intricate rather than a simple linear agglomeration process. The spatial variation in the LSI results from the superposition or interaction of multi-spatial location factors. The rural–urban spatial location factors are the key control element of the LSI and the interaction between rural–urban and local spatial location factors has the greatest influence on the LSI. It is simple for interconnected spatial location factors to produce a scale correlation effect, and have non-negligible effects on farmers’ livelihoods when they interact with other spatial location factors. Understanding the impact of rural location on farmers’ livelihood from a multi-spatial perspective is of great practical significance for identifying the causes of spatial heterogeneity in livelihoods and enhancing multi-level policy coordination on rural revitalization and livelihood security.
Epidemiology and Clinical Course of Haemorrhagic Fever with Renal Syndrome in New Endemic Area for Hantavirus Infection in Croatia
Authors: Cekinović Grbeša Đurđica , Zahirović Nino , Flego Viktorija , Livajić Marija , Rončević Filipović Mari , Knežević Samira , Slavuljica Irena
Published: August 2023

Background: Hantaviruses remain an important case of emerging and re-emerging infections in human medicine. This study aimed to analyse the epidemiology, clinical presentation, and outcome of hantavirus infections in the western part of Republic of Croatia, a new geographical area for hantavirus infections. Methods: Retrospective analysis of medical records of patients treated for hemorrhagic fever with renal syndrome (HFRS) at the infectious diseases Clinic of the Clinical Hospital Center in Rijeka, Croatia, from 1 January 2014, to 31 December 2021. Results: During the eight-year period, 251 patients were hospitalized and treated for HFRS, with epidemic outbreaks in years 2014 and 2021. Most patients had a typical clinical course of HFRS and received supportive care. Serological analysis revealed the Puumala Virus (PUUV) as the predominant etiology of the disease. Epidemiological analysis revealed clustering of infections in the region of Gorski Kotar and spread to the area on the Mediterranean coast (Adriatic Sea), which was previously considered an area free from hantavirus infections. Conclusions: The presented results indicate the spread of hantavirus infections in Croatia from the central low-lying parts of the country to the tourist-attractive western area adjacent to the Mediterranean coast, which was previously considered free of hantavirus infections.
Management of Cerebrospinal Fluid Leakage after Microvascular Decompression Surgery: Clinical Strategy
Authors: Lee Hyun-Seok , Cho Kyung-Rae , Park Kwan , Jeon Chiman
Published: August 2023

(1) Background: Cerebrospinal fluid (CSF) leakage is one of the most common complications of microvascular decompression (MVD) surgery. Before fatal complications, such as intracranial infection, occur, early recognition and prompt treatment are essential. (2) Methods: The clinical data of 475 patients who underwent MVD surgery from September 2020 to March 2023 were retrospectively analyzed. In these patients, if there were any symptoms of CSF leakage, and if CSF leakage was evident, a lumbar drainage catheter was inserted immediately. (3) Results: CSF leakage was suspected in 18 (3.8%) patients. Five of these patients (1.1%) showed signs of CSF leakage during conservative management and subsequently underwent catheter insertion for lumbar drainage. The lumbar drain was removed after an average of 5.2 days, resulting in an average hospitalization of 14.8 days. In all 5 patients, CSF leakage was resolved without reoperation. (4) Conclusions: Our treatment strategy prevented the development of fatal complications. Close observation of the symptoms and postoperative temporal bone computed tomography and audiometry are considered to be good evaluation methods for all patients. If CSF leakage is certain, it is important to perform lumbar drainage immediately.
Influence of Concentration of Sodium Metasilicate and Descaling on the High Temperature Lubricating Effects Evaluated by Hot Rolling Mill
Authors: Liu Hongliang , Wu Xun , Huang Jiaxuan , Shao Xibo , Wang Pei , Deng Guanyu , Wang Long
Published: August 2023

Lubricant is vital to improve energy efficiency and workpiece durability for the moving counterpart. High-temperature lubricants are important for the hot rolling process to reduce the rolling force and protect the roller and the strips. The current paper concerns eco-friendly sodium metasilicate as a high-temperature lubricant. A hot rolling mill is employed to evaluate the lubrication effect of sodium metasilicate. The influence of crucial factors of concentration of lubricant and descaling is discussed; the rolled surface was analyzed by scanning electron microscopy, energy dispersive spectroscopy, and 3D profilometer. The results depict that the sodium metasilicate can reduce the rolling force by about 7.8% when the concentration of sodium metasilicate is 18% and above, and descaling of the hot stripe makes the lubrication effect more effective, which can reach a 12.7% reduction in the rolling force. This lubrication is attributed to the formed melts of the sodium silicate layer that offers an easy shearing interface. For the un-descaled samples, the lubricant will be compacted and mixed with the oxide scale, and weakens the lubrication effect. This work suggests that sodium metasilicate can be a high-temperature lubricant for hot rolling; descaling is vital, not only for the quality of the product but also for the efficiency of the lubricant. This work will also be useful for the concentration selection of glass lubricant.
Numerical Investigation of Background Noise in a Circulating Water Tunnel
Authors: Huang Zhangkai , Chen Meixia , Wang Ting , Cui Huachang , Dong Wenkai
Published: August 2023

The presence of excessive background noise in hydrodynamic noise experiments conducted in circulating water tunnels can significantly impact the accuracy and reliability of experimental test results. To address this issue, it is crucial to evaluate and optimize the background noise during the design stage. In this research, acoustic field model and fluid–solid coupling numerical calculation model of circulating water tunnels are established. Utilizing the finite element method, we analyze the flow noise and flow-excited noise resulting from wall pressure pulses in the circulating water tunnel. Furthermore, we conduct a noise contribution analysis and explore strategies for structural vibration noise control. The results demonstrate that both flow noise and flow-excited noise decrease with increasing frequency, with flow-excited noise being the primary component of the tunnel’s background noise. The presence of resonant peaks significantly contributes to the elevated flow-excited noise levels. Moreover, enhancing structural stiffness and damping proves less effective in suppressing low-frequency peaks. Additionally, employing sound measurement pods suspended from the side of the test section for noise measurement exhibits a high error rate at low frequencies. This research provides insights into optimizing background noise in water tunnels, thereby informing future enhancements in tunnel design.
Expansion of MK Circle Theory for Dyads and Triads
Authors: Mather Sean , Erdman Arthur
Published: August 2023

The MK circles represent a kinematic synthesis tool for the dimensional synthesis of planar dyads. The tool is uniquely useful in its ability to both find specific dyad solutions and help the designer visualize numerous potential dyad pivot locations in the solution space. Here, the existing understanding of MK circles is summarized for three and four specified motion positions and extended for additional positions. Then the technique is expanded to show its application to MKT circles for triad synthesis, including solution space visualization, ground pivot specification, and multi-loop synthesis of complex mechanisms. These methods are illustrated by a unifying example that provides a sample procedure for applying the MK/MKT circles, and implements each of the aforementioned techniques. The interchangeability of loop-based synthesis approaches is demonstrated by comparing the new methodology to the compatibility linkages technique.
Adaptive Consensus of the Stochastic Leader-Following Multi-Agent System with Time Delay
Authors: Jin Shoubo , Zhang Guanghui
Published: August 2023

For the multi-agent system with time delay and noise, the adaptive consensus of tracking control problems is discussed by the Lyapunov function. The main purpose of this study is to design an adaptive control protocol for the system, such that even if there exists time delay among agents, the protocol can still ensure the consensus of the stochastic system. The main contribution is to revise the protocols that were previously only applicable to system without time delay. Because the system is inevitably disrupted by time delay and noise during the interactive process, achieving coordination and consensus is difficult. To enable the followers to track the leader, a novel adaptive law depending on the Riccati equation is firstly proposed, and the adaptive law is different from previous mandatory control law completely depending on a known function. The ability to be altered online based on the state of system is a major feature of the adaptive law. When there are interactive noise and time delay between the followers and leader of the system, a special Lyapunov function is constructed to prove the adaptive consensus. And the upper bound of time delay is obtained by using the Itô integral theory. Finally, if the time delay of the system approaches zero, it is shown that the adaptive law still ensures that each follower tracks the leader under simpler conditions.
Optimization Models for the Vehicle Routing Problem under Disruptions
Authors: Huang Kai , Xu Michael
Published: August 2023

In this paper, we study the role of disruptions in the multi-period vehicle routing problem (VRP), which naturally arises in humanitarian logistics and military applications. We assume that at any time during the delivery phase, each vehicle could have chance to be disrupted. When a disruption happens, vehicles will be unable to continue their journeys and supplies will be unable to be delivered. We model the occurrence of disruption as a given probability and consider the multi-period expected delivery. Our objective is to either minimize the total travel cost or maximize the demand fulfillment, depending on the supply quantity. This problem is denoted as the multi-period vehicle routing problem with disruption (VRPMD). VRPMD does not deal with disruptions in real-time and is more focused on the long-term performance of a single routing plan. We first prove that the proposed VRPMD problems are NP-hard. We then present some analytical properties related to the optimal solutions to these problems. We show that Dror and Trudeau’s property does not apply in our problem setting. Nevertheless, a generalization of Dror and Trudeau’s property holds. Finally, we present efficient heuristic algorithms to solve these problems and show the effectiveness of the proposed models and algorithms through numerical studies.
Effective Improved NSGA-II Algorithm for Multi-Objective Integrated Process Planning and Scheduling
Authors: Wen Xiaoyu , Song Qingbo , Qian Yunjie , Qiao Dongping , Wang Haoqi , Zhang Yuyan , Li Hao
Published: August 2023

Integrated process planning and scheduling (IPPS) is important for modern manufacturing companies to achieve manufacturing efficiency and improve resource utilization. Meanwhile, multiple objectives need to be considered in the realistic decision-making process for manufacturing systems. Based on the above realistic manufacturing system requirements, it becomes increasingly important to develop effective methods to deal with multi-objective IPPS problems. Therefore, an improved NSGA-II (INSGA-II) algorithm is proposed in this research, which uses the fast non-dominated ranking method for multiple optimization objectives as an assignment scheme for fitness. A multi-layer integrated coding method is adopted to address the characteristics of the integrated optimization model, which involves many optimization parameters and interactions. Elite and mutation strategies are employed during the evolutionary process to enhance population diversity and the quality of solutions. An external archive is also used to store and update the Pareto solution. The experimental results on the Kim test set demonstrate the effectiveness of the proposed INSGA-II algorithm.
Generalized Taylor’s Formula and Steffensen’s Inequality
Authors: Fahad Asfand , Butt Saad Ihsaan , Pečarić Josip , Praljak Marjan
Published: August 2023

New Steffensen-type inequalities are obtained by combining generalized Taylor expansions, Rabier and Pečarić extensions of Steffensen’s inequality and Faà di Bruno’s formula for higher order derivatives of the composition.
Fatigue Estimation Using Peak Features from PPG Signals
Authors: Chen Yi-Xiang , Tseng Chin-Kun , Kuo Jung-Tsung , Wang Chien-Jen , Chao Shu-Hung , Kau Lih-Jen , Hwang Yuh-Shyan , Lin Chun-Ling
Published: August 2023

Fatigue is a prevalent subjective sensation, affecting both office workers and a significant global population. In Taiwan alone, over 2.6 million individuals—around 30% of office workers—experience chronic fatigue. However, fatigue transcends workplaces, impacting people worldwide and potentially leading to health issues and accidents. Gaining insight into one’s fatigue status over time empowers effective management and risk reduction associated with other ailments. Utilizing photoplethysmography (PPG) signals brings advantages due to their easy acquisition and physiological insights. This study crafts a specialized preprocessing and peak detection methodology for PPG signals. A novel fatigue index stems from PPG signals, focusing on the dicrotic peak’s position. This index replaces subjective data from the brief fatigue index (BFI)-Taiwan questionnaire and heart rate variability (HRV) indices derived from PPG signals for assessing fatigue levels. Correlation analysis, involving sixteen healthy adults, highlights a robust correlation (R > 0.53) between the new fatigue index and specific BFI questions, gauging subjective fatigue over the last 24 h. Drawing from these insights, the study computes an average of the identified questions to formulate the evaluated fatigue score, utilizing the newfound fatigue index. The implementation of linear regression establishes a robust fatigue assessment system. The results reveal an impressive 91% correlation coefficient between projected fatigue levels and subjective fatigue experiences. This underscores the remarkable accuracy of the proposed fatigue prediction in evaluating subjective fatigue. This study further operationalized the proposed PPG processing, peak detection method, and fatigue index using C# in a computer environment alongside a PPG device, thereby offering real-time fatigue indices to users. Timely reminders are employed to prompt users to take notice when their index exceeds a predefined threshold, fostering greater attention to their physical well-being.
Vertigo Associated with Otosclerosis and Stapes Surgery—A Narrative Review
Authors: Necula Violeta , Maniu Alma Aurelia , Ujváry László-Péter , Dindelegan Maximilian-George , Tănase Mara , Tănase Mihai , Blebea Cristina Maria
Published: August 2023

Otosclerosis is a pathological condition affecting the temporal bone, and is characterized by remodelling of the labyrinthine bone tissue through a dynamic process of osteolysis and osteogenesis. This condition progressively leads to hearing loss, tinnitus, and vertigo. Stapedotomy, a surgical procedure involving the removal of the stapes superstructure and its replacement with a prosthesis, is the treatment of choice to improve hearing in individuals with otosclerosis. However, vestibular dysfunction is a significant complication associated with this procedure, which can occur intraoperatively or postoperatively, ranging from the immediate postoperative period to weeks, months, or even years after surgery. This paper aims to provide a comprehensive review of the most important causes of vertigo associated with otosclerosis and stapes surgery with the goal of minimizing the incidence of this complication. Understanding the underlying factors contributing to vertigo in this context is crucial for the prevention and effective management of vertigo in patients undergoing stapedotomy.
Overview of Ankle Arthropathy in Hereditary Hemochromatosis
Authors: Calori Sara , Comisi Chiara , Mascio Antonio , Fulchignoni Camillo , Pataia Elisabetta , Maccauro Giulio , Greco Tommaso , Perisano Carlo
Published: August 2023

Hereditary hemochromatosis (HH) is an autosomal recessive bleeding disorder characterized by tissue overload of iron. Clinical systemic manifestations in HH include liver disease, cardiomyopathy, skin pigmentation, diabetes mellitus, erectile dysfunction, hypothyroidism, and arthropathy. Arthropathy with joint pain is frequently reported at diagnosis and mainly involves the metacarpophalangeal and ankle joints, and more rarely, the hip and knee. Symptoms in ankle joints are in most cases non-specific, and they can range from pain and swelling of the ankle to deformities and joint destruction. Furthermore, the main radiological signs do not differ from those of primary osteoarthritis (OA). Limited data are available in the literature regarding treatment; surgery seems to be the gold standard for ankle arthropathy in HH. Pharmacological treatments used to maintain iron homeostasis can also be undertaken to prevent the arthropathy, but conclusive data are not yet available. This review aimed to assess the ankle arthropathy in the context of HH, including all its aspects: epidemiology, physiopathology, clinical and imaging presentation, and all the treatments available to the current state of knowledge.
Influence of Different Filler Metals on the Mechanical and Microstructural Characteristics of Arc-Welded Joints Made of Dissimilar Titanium Alloys
Authors: Gaiani Silvia , Gozzi Marica , Ferrari Elisa , Menozzi Alberto , Lassinantti Gualtieri Magdalena , Colombini Elena , Veronesi Paolo
Published: August 2023

In the motorsport industry, the choice of material for manufacturing the heat resistant components often falls on titanium alloys. In most cases, the production flow for this kind of part involves CNC machining and subsequent assembly by welding process, to other parts obtained by cold plastic forming and possibly made using different titanium alloys. Hence, the alloying element-content in the joint area can be extremely heterogeneous and variable point-by-point. To investigate this topic further, dissimilar welding of the alpha/beta alloy Ti6Al4V and of the oxidation-resistant alpha alloy KS-Ti 1.2 ASN-EX was made by GTAW technology and using different filler metals. Chemical and mechanical properties of the welds were investigated by XRD, SEM-EDS, microhardness maps, and tensile and bending tests. Results show that, despite the different alloying elements present in the two filler wires investigated, static properties of the welds are similar. Results also show that the local V/Al content ratio affects the microhardness as it is responsible for the creation of supersaturated alpha phases during the cooling of the weld beads.
Enhancing Mechanical and Biocorrosion Response of a MgZnCa Bulk Metallic Glass through Variation in Spark Plasma Sintering Time
Authors: Bryan Bin Shi Jie , Fong Kai Soon , Wah Chua Beng , Tekumalla Sravya , Kwak Min Kyung , Park Eun Soo , Gupta Manoj
Published: August 2023

Development of metallic glasses is hindered by the difficulties in manufacturing bulk parts large enough for practical applications. Spark plasma sintering (SPS) has emerged as an effective consolidation technique in the formation of bulk metallic glasses (BMGs) from melt-spun ribbons. In this study, Mg65Zn30Ca5 melt-spun ribbons were sintered at prolonged sintering times (15 min to 180 min) via SPS under a pressure of 90 MPa and at a temperature of 150 °C (which is below the crystallization temperature), to provide an insight into the influence of sintering time on the consolidation, structural, and biodegradation behavior of Mg-BMGs. Scanning Electron Microscopy was used to characterize the microstructure of the surface, while the presence of the amorphous phase was characterized using X-ray diffraction and Electron Backscatter Diffraction. Pellets 10 mm in diameter and height with near-net amorphous structure were synthesized at 150 °C with a sintering time of 90 min, resulting in densification as high as 98.2% with minimal crystallization. Sintering at extended durations above 90 min achieved higher densification and resulted in a significant amount of local and partial devitrification. Mechanical properties were characterized via compression and microhardness testing. Compression results show that increased sintering time led to better structural integrity and mechanical properties. Notably, SPS150_90 displayed ultimate compressive strength (220 MPa) that matches that of the cortical bone (205 MPa). Corrosion properties were characterized via potentiodynamic polarization with Phosphate Buffered Solution (PBS). The results suggest that the sintered samples have significantly better corrosion resistance compared to the crystalline form. Overall, SPS150_90 was observed to have a good balance between corrosion properties (10× better corrosion resistance to as-cast alloy) and mechanical properties.
Effects of Gold Nanoparticles Phytoreduced with Rutin in an Early Rat Model of Diabetic Retinopathy and Cataracts
Authors: Moldovan Mădălina , Păpurică Ana-Maria , Muntean Mara , Bungărdean Raluca Maria , Gheban Dan , Moldovan Bianca , Katona Gabriel , David Luminița , Filip Gabriela Adriana
Published: August 2023

Diabetic retinopathy (DR) and cataracts (CA) have an early onset in diabetes mellitus (DM) due to the redox imbalance and inflammation triggered by hyperglycaemia. Plant-based therapies are characterised by low tissue bioavailability. The study aimed to investigate the effect of gold nanoparticles phytoreduced with Rutin (AuNPsR), as a possible solution. Insulin, Rutin, and AuNPsR were administered to an early, six-week rat model of DR and CA. Oxidative stress (MDA, CAT, SOD) was assessed in serum and eye homogenates, and inflammatory cytokines (IL-1 beta, IL-6, TNF alpha) were quantified in ocular tissues. Eye fundus of retinal arterioles, transmission electron microscopy (TEM) of lenses, and histopathology of retinas were also performed. DM was linked to constricted retinal arterioles, reduced endogen antioxidants, and eye inflammation. Histologically, retinal wall thickness decreased. TEM showed increased lens opacity and fibre disorganisation. Rutin improved retinal arteriolar diameter, while reducing oxidative stress and inflammation. Retinas were moderately oedematous. Lens structure was preserved on TEM. Insulin restored retinal arteriolar diameter, while increasing MDA, and amplifying TEM lens opacity. The best outcomes were obtained for AuNPsR, as it improved fundus appearance of retinal arterioles, decreased MDA and increased antioxidant capacity. Retinal edema and disorganisation in lens fibres were still present.
Metabolic Syndrome in Affective Disorders: Associations with Dark Triad Personality Traits
Authors: Brugger Fiona , Schönthaler Elena M. D. , Baranyi Andreas , Reininghaus Eva Z. , von Lewinski Dirk , Dalkner Nina
Published: August 2023

Previous research has focused on the relationship between affective disorders (AD) and metabolic syndrome (MetS). Aside from biological and lifestyle factors, personality traits were identified as influencing aspects. In particular, the Dark Triad personality traits (DT; Machiavellianism, narcissism, psychopathy) were connected to both AD and worse somatic health, thus possibly resulting in MetS. This observational study aimed to investigate the associations between DT and anthropometric parameters and differences in the DT traits concerning the presence of MetS in individuals with AD. A total of 112 individuals (females = 59, males = 51, diverse = 2, Mage = 47.5, SDage = 11.5) with AD filled out the Short Dark Triad questionnaire. Body Mass Index (BMI) and MetS criteria, including blood pressure, waist circumference, lipid, and glucose levels, were assessed. For Machiavellianism, a positive association with BMI (r = 0.29, p < 0.05) and a negative association with systolic blood pressure (r = −0.23, p < 0.05) were found. No relationship between the overall MetS and DT score (r = 0.08, p = 0.409) was observed. The results were limited by the lack of a control group and the cross-sectional study design, which does not allow for the determination of causality. Machiavellianism was associated with a higher BMI and lower systolic blood pressure, indicating a deteriorating health effect of this trait. Possibly, the higher prevalence of MetS in AD stems from aspects such as lifestyle or medication intake, which might also be influenced by DT. Further research is needed to disentangle underlying mechanisms.
Influence of Heat Stress on Body Temperatures Measured by Infrared Thermography, Blood Metabolic Parameters and Its Correlation in Sheep
Authors: Čukić Aleksandar , Rakonjac Simeon , Djoković Radojica , Cincović Marko , Bogosavljević-Bošković Snežana , Petrović Milun , Savić Željko , Andjušić Ljiljana , Andjelić Biljana
Published: August 2023

The aim of this research is to examine the influence of heat stress (HS) on body temperature (BT) measured rectally (RT) or by infrared thermography (IRT) of the nose (NT), eye (ET), leg (LT) and abdominal (AT) regions in intensively and extensively breed sheep and to detect a correlation between body temperature and metabolic response in sheep. A total of 33 Wurttemberg × Sjenica Pramenka sheep breeds were examined, 17 ewes were from outdoors and 16 were from indoor housing systems during three experimental periods (thermoneutral period, severe HS and moderate HS). Sheep under HS have a higher BT, and the magnitude of BT measured by infrared thermography (IRT) was higher than RT. LT and AT showed positive linear correlations with the temperature–humidity index (THI), while other ways of measuring BT did not give statistically significant correlations. Sheep under HS showed higher cortisol, insulin, total protein, albumin, urea, creatinine, bilirubin, aspartate aminotransferase, alanine aminotransferase, gamma-glutamyl transferase, alkaline phosphatase, lactate dehydrogenase, creatine kinase and index of insulin resistance, with lower values of triiodothyronine (T3), thyroxine (T4), non-esterified fatty acids, beta-hydroxybutyrate (BHB), glucose, calcium, inorganic phosphates, magnesium and cholesterol. BT and metabolic response were different in the function of the housing method of sheep. LT and AT showed a significant correlation with almost all blood parameters, and the strongest connections were made with T3, T4, BHB and the revised quantitative insulin sensitivity check index of insulin resistance. The abdomen and legs are good thermal windows because LT and AT are good summative responses to external ambient THI and internal metabolic changes in sheep under heat stress.
Specnuezhenide Ameliorates Age-Related Hepatic Lipid Accumulation via Modulating Bile Acid Homeostasis and Gut Microbiota in D-Galactose-Induced Mice
Authors: Deng Xuehui , Lin Bingfeng , Wang Fang , Xu Pingcui , Wang Nani
Published: August 2023

Age-related hepatic lipid accumulation has become a major health problem in the elderly population. Specnuezhenide (SPN) is a major active iridoid glycoside from an edible herb Fructus Ligustri Lucidi, which is commonly used for preventing age-related diseases. However, the beneficial effects of SPN on age-related liver injury remain unknown. This study aimed to reveal the effect of SPN on age-related hepatic lipid accumulation and the underlying mechanism. D-galactose (D-gal)-induced aging mice were treated with vehicle or SPN for 12 weeks. Treatment of SPN decreased lipid accumulation and inflammation in the liver of D-gal–induced mice. Untargeted and targeted metabolomics showed that the SPN could regulate the bile acid (BA) synthesis pathway and restore the BA compositions in serum, livers, and feces of the D-gal–induced mice. Furthermore, SPN enhanced the protein and mRNA levels of hepatic BAs synthesis enzymes cytochrome P45027A1, cytochrome P4507A1, cytochrome P4507B1, and cytochrome P4508B1. Meanwhile, SPN alleviated D-gal-induced gut dysbiosis and reversed the proportions of microbes associated with bile salt hydrolase activity, including Lactobacillus, Ruminiclostridium, and Butyrivibrio. Our study revealed that SPN attenuated age-related hepatic lipid accumulation by improving BA profiles via modulating hepatic BA synthesis enzymes and gut microbiota.
Adsorption of Preformed Microgel–Enzyme Complexes as a Novel Strategy toward Engineering Microgel-Based Enzymatic Biosensors
Authors: Sigolaeva Larisa V. , Shalybkova Anna A. , Sharifullin Timur Z. , Pergushov Dmitry V.
Published: August 2023

A novel approach to surface modification, which consists of the adsorption of microgel–enzyme complexes preformed in solution, is highlighted. Accordingly, the microgel–enzyme complexes were formed due to the electrostatic interaction of the oppositely charged interacting components, that is, a cationic poly(N-isopropylacrylamide)-based microgel and glucose oxidase taken as a model enzyme. The spontaneous adsorption of the prepared microgel–enzyme complexes, examined by means of quartz crystal microbalance with dissipation monitoring and atomic force microscopy, was observed, resulting in the formation of well-adhered microgel–enzyme coatings. Further, the preformed microgel–enzyme complexes were adsorbed onto the modified graphite-based screen-printed electrodes, and their enzymatic responses were determined by means of amperometry, demonstrating a remarkable analytical performance toward the quantification of β-D-glucose in terms of high sensitivity (0.0162 A × M−1 × cm−2), a low limit of detection (1 μM), and an expanded linear range (1–2000 μM). The fabricated microgel–enzyme biosensor constructs were found to be very stable against manifold-repeated measurements. Finally, the pH- or salt-induced release of glucose oxidase from the adsorbed preformed microgel–enzyme complexes was demonstrated. The findings obtained for the microgel–enzyme coatings prepared via adsorption of the preformed microgel–enzyme complexes were compared to those found for the microgel–enzyme coatings fabricated via a previously exploited two-stage sequential adsorption, which includes the adsorption of the microgel first, followed by the electrostatic binding of glucose oxidase by the adsorbed microgel.
SW16-7, a Novel Ackermannviridae Bacteriophage with Highly Effective Lytic Activity Targets Salmonella enterica Serovar Weltevreden
Authors: Xu Jialiang , Li Jia , Yan Yi , Han Pengjun , Tong Yigang , Li Xu
Published: August 2023

Salmonella enterica serovar Weltevreden is a foodborne pathogen commonly transmitted through fresh vegetables and seafood. In this study, a lytic phage, SW16-7, was isolated from medical sewage, demonstrating high infectivity against S. Weltevreden, S. London, S. Meleagridis, and S. Give of Group O:3. In vitro inhibition assays revealed its effective antibacterial effect for up to 12 h. Moreover, analysis using the Comprehensive Antibiotic Resistance Database (CARD) and the Virulence Factor Database (VFDB) showed that SW16-7’s genome does not contain any virulence factors or antibiotic resistance genes, indicating its potential as a promising biocontrol agent against S. Weltevreden. Additionally, a TSP gene cluster was identified in SW16-7’s genome, with TSP1 and TSP2 showing a high similarity to lysogenic phages ε15 and ε34, respectively, in the C-terminal region. The whole-genome phylogenetic analysis classified SW16-7 within the Ackermannviridae family and indicated a close relationship with Agtrevirus, which is consistent with the ANI results.
Infant Saliva Microbiome Activity Modulates Nutritional Impacts on Neurodevelopment
Authors: Keck-Kester Terrah , Hicks Steven D.
Published: August 2023

Neurodevelopment is influenced by complex interactions between environmental factors, including social determinants of health (SDOH), nutrition, and even the microbiome. This longitudinal cohort study of 142 infants tested the hypothesis that microbial activity modulates the effects of nutrition on neurodevelopment. Salivary microbiome activity was measured at 6 months using RNA sequencing. Infant nutrition was assessed longitudinally with the Infant Feeding Practices survey. The primary outcome was presence/absence of neurodevelopmental delay (NDD) at 18 months on the Survey of Wellbeing in Young Children. A logistic regression model employing two microbial factors, one nutritional factor, and two SDOH accounted for 33.3% of the variance between neurodevelopmental groups (p < 0.001, AIC = 77.7). NDD was associated with Hispanic ethnicity (OR 18.1, 2.36–139.3; p = 0.003), no fish consumption (OR 10.6, 2.0–54.1; p = 0.003), and increased Candidatus Gracilibacteria activity (OR 1.43, 1.00–2.07; p = 0.007). Home built after 1977 (OR 0.02, 0.001–0.53; p = 0.004) and Chlorobi activity (OR 0.76, 0.62–0.93, p = 0.001) were associated with reduced risk of NDD. Microbial alpha diversity modulated the effect of fish consumption on NDD (X2 = 5.7, p = 0.017). These data suggest the benefits of fish consumption for neurodevelopment may be mediated by microbial diversity. Confirmation in a larger, randomized trial is required.
Effects and Stress-Relieving Mechanisms of Dark Tea Polysaccharide in Human HaCaT Keratinocytes and SZ95 Sebocytes
Authors: Gao Chang , Fu Jiafeng , Cui Junyi , Zhang Tingzhi , Zouboulis Christos C. , Wang Jing , Yan Shaowei
Published: August 2023

A new skincare application scenario for dark tea, a unique and post-fermented tea popular in the health food industry, was developed in this paper. The effects of dark tea polysaccharide (DTP) on stress-induced skin problems and its mechanism of action were investigated by modeling cortisone-induced stress injury in human HaCaT keratinocytes and SZ95 sebaceous gland cells. The results showed a reduced cortisol conversion induced by cortisone under the action of DTP with a concentration of 200 μg/mL, probably by inhibiting the expression of the HSD11B1 enzyme. DTP was also able to suppress the cortisone-induced elevation of lipid levels in SZ95 sebocytes at this concentration. In addition, the composition and structure of DTP were verified by ultrafiltration, ultraviolet-visible spectrophotometry (UV-VIS), high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and infrared spectroscopy. In brief, DTP has a unique and significant stress-relieving effect, which provides new ideas for the development of new ingredients for the skin care industry.
Recent Advances in Fabrication of Durable, Transparent, and Superhydrophobic Surfaces
Authors: Luo Wenxin , Li Mingjie
Published: August 2023

Transparent superhydrophobic coatings have been extensively investigated due to their ability to provide self-cleaning properties for outdoor applications. However, the widespread implementation of these coatings on a large scale is impeded by the challenges of poor durability and complex fabrication procedures. In this review, the fundamentals and theories governing the mutually exclusive properties of superhydrophobicity, optical transparency, and susceptibility to wear are introduced, followed by a discussion of representative examples of advanced surface design and processing optimizations. Also, robust evaluation protocols for assessing mechanical and chemical stabilities are briefed and potential research directions are presented. This review can offer the research community a better understanding of durable and transparent superhydrophobic surfaces, thereby facilitating their development for real-world applications.
Delivery of Therapeutic miRNA via Plasma-Polymerised Nanoparticles Rescues Diabetes-Impaired Endothelial Function
Authors: Lam Yuen Ting , Lee Bob S. L. , Hung Juichien , Michael Praveesuda , Santos Miguel , Tan Richard P. , Liu Renjing , Wise Steven G.
Published: August 2023

MicroRNAs (miRNAs) are increasingly recognised as key regulators of the development and progression of many diseases due to their ability to modulate gene expression post-translationally. While this makes them an attractive therapeutic target, clinical application of miRNA therapy remains at an early stage and in part is limited by the lack of effective delivery modalities. Here, we determined the feasibility of delivering miRNA using a new class of plasma-polymerised nanoparticles (PPNs), which we have recently isolated and characterised. We showed that PPN-miRNAs have no significant effect on endothelial cell viability in vitro in either normal media or in the presence of high-glucose conditions. Delivery of a miRNA inhibitor targeting miR-503 suppressed glucose-induced miR-503 upregulation and restored the downstream mRNA expression of CCNE1 and CDC25a in endothelial cells. Subsequently, PPN delivery of miR-503 inhibitors enhanced endothelial angiogenesis, including tubulogenesis and migration, in culture conditions that mimic diabetic ischemia. An intramuscular injection of a PPN-miR-503 inhibitor promoted blood-perfusion recovery in the hindlimb of diabetic mice following surgically induced ischemia, linked with an increase in new blood vessel formation. Together, this study demonstrates the effective use of PPN to deliver therapeutic miRNAs in the context of diabetes.