bluuebunny/crossref_metadata_2025 huggingface dataset:
Hippophae rhamnoides L. (sea buckthorn) mediated green synthesis of copper nanoparticles and their application in anticancer activity
Authors: Dadhwal Pooja , Dhingra Harish Kumar , Dwivedi Vinay , Alarifi Saud , Kalasariya Haresh , Yadav Virendra Kumar , Patel Ashish
Published: August 2023

Green synthesis of nanoparticles has drawn huge attention in the last decade due to their eco-friendly, biocompatible nature. Phyto-assisted synthesis of metallic nanoparticles is widespread in the field of nanomedicine, especially for antimicrobial and anticancer activity. Here in the present research work, investigators have used the stem extract of the Himalayan plant Hippophae rhamnoides L, for the synthesis of copper nanoparticles (CuNPs). The synthesized of CuNPs were analyzed by using sophisticated instruments, i.e., Fourier transform infrared spectroscopy (FTIR), UV-Vis spectroscopy, X-ray diffraction (XRD), high-performance liquid chromatography (HPLC), and scanning electron microscope (SEM). The size of the synthesized CuNPs was varying from 38 nm to 94 nm which were mainly spherical in shape. Further, the potential of the synthesized CuNPs was evaluated as an anticancer agent on the Hela cell lines, by performing an MTT assay. In the MTT assay, a concentration-dependent activity of CuNPs demonstrated the lower cell viability at 100 μg/mL and IC50 value at 48 μg/mL of HeLa cancer cell lines. In addition to this, apoptosis activity was evaluated by reactive oxygen species (ROS), DAPI (4′,6-diamidino-2-phenylindole) staining, Annexin V, and Propidium iodide (PI) staining, wherein the maximum ROS production was at a dose of 100 µg per mL of CuNPs with a higher intensity of green fluorescence. In both DAPI and PI staining, maximum nuclear condensation was observed with 100 μg/mL of CuNPs against HeLa cell lines.
Heterogeneity in macrophages along the cochlear spiral in mice: insights from SEM and functional analyses
Authors: Zhang Celia , Ye Mengxiao , Bush Peter , Hu Bo Hua
Published: August 2023

The susceptibility of sensory cells to pathological conditions differs between the apical and basal regions of the cochlea, and the cochlear immune system may contribute to this location-dependent variability. Our previous study found morphological differences in basilar membrane macrophages between the apical and basal regions of the cochlea. However, the details of this site-dependent difference and its underlying structural and biological basis are not fully understood. In this study, we utilized scanning electron microscopy to examine the ultrastructure of macrophages and their surrounding supporting structures. Additionally, we examined the phagocytic activities of macrophages and the expression of immune molecules in both apical and basal regions of the cochlea. We employed two mouse strains (C57BL/6J and B6.129P-Cx3cr1tm1Litt/J) and evaluated three experimental conditions: young normal (1–4 months), aging (11–19 months), and noise-induced damage (120 dB SPL for 1 h). Using scanning electron microscopy, we revealed location-specific differences in basilar membrane macrophage morphology and surface texture, architecture in mesothelial cell layers, and spatial correlation between macrophages and mesothelial cells in both young and older mice. Observations of macrophage phagocytic activities demonstrated that basal macrophages exhibited greater phagocytic activities in aging and noise-damaged ears. Furthermore, we identified differences in the expression of immune molecules between the apical and basal cochlear tissues of young mice. Finally, our study demonstrated that as the cochlea ages, macrophages in the apical and basal regions undergo a transformation in their morphologies, with apical macrophages acquiring certain basal macrophage features and vice versa. Overall, our findings demonstrate apical and basal differences in macrophage phenotypes and functionality, which are related to distinct immune and structural differences in the macrophage surrounding tissues.
Measured sodium excretion is associated with cardiovascular outcomes in non-dialysis CKD patients: results from the KNOW-CKD study
Authors: Kang Seong Cheol , Kang Minjung , Ryu Hyunjin , Kim Seonmi , Kim Ji Hye , Kang Eunjeong , Jeong Yujin , Kim Jayoun , Kim Yong-Soo , Kim Soo Wan , Kim Yeong Hoon , Oh Kook-Hwan
Published: August 2023

Background

There are insufficient studies on the effect of dietary salt intake on cardiovascular (CV) outcomes in chronic kidney disease (CKD) patients, and there is no consensus on the sodium (Na) intake level that increases the risk of CV disease in CKD patients. Therefore, we investigated the association between dietary salt intake and CV outcomes in CKD patients.

Methods

In the Korean cohort study for Outcome in patients with CKD (KNOW-CKD), 1,937 patients were eligible for the study, and their dietary Na intake was estimated using measured 24h urinary Na excretion. The primary outcome was a composite of CV events and/or all-cause death. The secondary outcome was a major adverse cardiac event (MACE).

Results

Among 1,937 subjects, there were 205 (10.5%) events for the composite outcome and 110 (5.6%) events for MACE. Compared to the reference group (urinary Na excretion< 2.0g/day), the group with the highest measured 24h urinary Na excretion (urinary Na excretion ≥ 8.0g/day) was associated with increased risk of both the composite outcome (hazard ratio 3.29 [95% confidence interval 1.00-10.81]; P = 0.049) and MACE (hazard ratio 6.28 [95% confidence interval 1.45-27.20]; P = 0.013) in a cause-specific hazard model. Subgroup analysis also showed a pronounced association between dietary salt intake and the composite outcome in subgroups of patients with abdominal obesity, female, lower estimated glomerular filtration rate (< 60 ml/min per 1.73m2), no overt proteinuria, or a lower urinary potassium-to-creatinine ratio (< 46 mmol/g).

Conclusion

A high-salt diet is associated with CV outcomes in non-dialysis CKD patients.
Reactive gliosis and neuroinflammation: prime suspects in the pathophysiology of post-acute neuroCOVID-19 syndrome
Authors: Saucier Jacob , Comeau Dominique , Robichaud Gilles A. , Chamard-Witkowski Ludivine
Published: August 2023

Introduction

As the repercussions from the COVID-19 pandemic continue to unfold, an ever-expanding body of evidence suggests that infection also elicits pathophysiological manifestations within the central nervous system (CNS), known as neurological symptoms of post-acute sequelae of COVID infection (NeuroPASC). Although the neurological impairments and repercussions associated with NeuroPASC have been well described in the literature, its etiology remains to be fully characterized.

Objectives

This mini-review explores the current literature that elucidates various mechanisms underlining NeuroPASC, its players, and regulators, leading to persistent neuroinflammation of affected individuals. Specifically, we provide some insights into the various roles played by microglial and astroglial cell reactivity in NeuroPASC and how these cell subsets potentially contribute to neurological impairment in response to the direct or indirect mechanisms of CNS injury.

Discussion

A better understanding of the mechanisms and biomarkers associated with this maladaptive neuroimmune response will thus provide better diagnostic strategies for NeuroPASC and reveal new potential mechanisms for therapeutic intervention. Altogether, the elucidation of NeuroPASC pathogenesis will improve patient outcomes and mitigate the socioeconomic burden of this syndrome.
Barriers and facilitators for healthcare providers to implement family-centered care in Parkinson's disease: a scoping review
Authors: Sun Wen-Jing , Peng Ye-Jie , Liang Yan
Published: August 2023

Aims

This study aimed to identify and synthesize the barriers and facilitators to the implementation of family-centered care in Parkinson's disease (PD) and to provide a reference for evidence-based Parkinson's disease nursing practice.

Methods

This scoping review follows the methodology framework proposed by Arksey and O'Malley. Four databases including PubMed, Web of Science, Embase, and Cochrane Library were searched. Barriers and facilitators were summarized based on the ecological family-centered model.

Results

Through a comprehensive literature search, 35 studies were found for this scoping review. Barriers and facilitators to implementing family-centered care in PD included physiological factors, environmental factors, culturally based conflicts, living arrangements, education or skills training, group experiences, and individual and family consultations.

Conclusion

Implementing family-centered care in Parkinson's disease is essential to providing comprehensive care that improves outcomes for both PD patients and their family members.
Predictive factors for Alzheimer’s disease progression: a comprehensive retrospective analysis of 3,553 cases with 211 months follow-up
Authors: Özge Aynur , Ghouri Reza , Öksüz Nevra , Taşdelen Bahar
Published: August 2023

Background

There is conflicting data regarding the predictors of Alzheimer’s Disease (AD), the most common form of dementia. The main objective of the study is to evaluate potential predictors of AD progression using a comprehensive follow-up dataset that includes functional/cognitive assessments, clinical and neuropsychiatric evaluations, and neuroimaging biomarkers such as hippocampal atrophy or white matter intensities (WMIs).

Method

A total of 161 AD cases were recruited from a dementia database consisting of individuals who consulted the Dementia Outpatient Clinic of the Neurology Department at Mersin University Medical Faculty between 2000 and 2022, under the supervision of the same senior author have at least 3 full evaluation follow-up visit including functional, clinical, biochemical, neuropsychological, and radiological screening. Data were exported and analyzed by experts accordingly.

Results

Mean follow-up duration of study sample was 71.66 ± 41.98, min 15 to max 211 months. The results showed a fast and slow progressive subgroup of our AD cases with a high sensitivity (Entropy = 0.836), with a close relationship with several cofactors and the level of disability upon admittance. Hippocampal atrophy and WMIs grading via Fazekas were found to be underestimated predictors of AD progression, and functional capacity upon admittance was also among the main stakeholders.

Conclusion

The study highlights the importance of evaluating multiple potential predictors for AD progression, including functional capacity upon admittance, hippocampal atrophy, and WMIs grading via Fazekas. Our findings provide insight into the complexity of AD progression and may contribute to the development of effective strategies for managing and treating AD.
Sensitivity analysis of point neuron model simulations implemented on neuromorphic hardware
Authors: Dey Srijanie , Dimitrov Alexander G.
Published: August 2023

With the ongoing growth in the field of neuro-inspired computing, newly arriving computational architectures demand extensive validation and testing against existing benchmarks to establish their competence and value. In our work, we break down the validation step into two parts—(1) establishing a methodological and numerical groundwork to establish a comparison between neuromorphic and conventional platforms and, (2) performing a sensitivity analysis on the obtained model regime to assess its robustness. We study the neuronal dynamics based on the Leaky Integrate and Fire (LIF) model, which is built upon data from the mouse visual cortex spanning a set of anatomical and physiological constraints. Intel Corp.'s first neuromorphic chip “Loihi” serves as our neuromorphic platform and results on it are validated against the classical simulations. After setting up a model that allows a seamless mapping between the Loihi and the classical simulations, we find that Loihi replicates classical simulations very efficiently with high precision. This model is then subjected to the second phase of validation, through sensitivity analysis, by assessing the impact on the cost function as values of the significant model parameters are varied. The work is done in two steps—(1) assessing the impact while changing one parameter at a time, (2) assessing the impact while changing two parameters at a time. We observe that the model is quite robust for majority of the parameters with slight change in the cost function. We also identify a subset of the model parameters changes which make the model more sensitive and thus, need to be defined more precisely.
Factors associated with the need of parenteral nutrition in critically ill patients after the initiation of enteral nutrition therapy
Authors: Lopez-Delgado Juan Carlos , Servia-Goixart Lluís , Grau-Carmona Teodoro , Bordeje-Laguna Luisa , Portugal-Rodriguez Esther , Lorencio-Cardenas Carolina , Vera-Artazcoz Paula , Macaya-Redin Laura , Martinez-Carmona Juan Francisco , Marin Corral Judith , Flordelís-Lasierra Jose Luis , Seron-Arbeloa Carlos , Alcazar-Espin Maravillas de las Nieves , Navas-Moya Elisabeth , Aldunate-Calvo Sara , Nieto Martino Beatriz , Martinez de Lagran Itziar
Published: August 2023

Background and aims

Despite enteral nutrition (EN) is the preferred route of nutrition in patients with critical illness, EN is not always able to provide optimal nutrient provision and parenteral nutrition (PN) is needed. This is strongly associated with gastrointestinal (GI) complications, a feature of gastrointestinal dysfunction and disease severity. The aim of the present study was to investigate factors associated with the need of PN after start of EN, together with the use and complications associated with EN.

Methods

Adult patients admitted to 38 Spanish intensive care units (ICUs) between April and July 2018, who needed EN therapy were included in a prospective observational study. The characteristics of EN-treated patients and those who required PN after start EN were analyzed (i.e., clinical, laboratory and scores).

Results

Of a total of 443 patients, 43 (9.7%) received PN. One-third (29.3%) of patients presented GI complications, which were more frequent among those needing PN (26% vs. 60%, p = 0.001). No differences regarding mean energy and protein delivery were found between patients treated only with EN (n = 400) and those needing supplementary or total PN (n = 43). Abnormalities in lipid profile, blood proteins, and inflammatory markers, such as C-Reactive Protein, were shown in those patients needing PN. Sequential Organ Failure Assessment (SOFA) on ICU admission (Hazard ratio [HR]:1.161, 95% confidence interval [CI]:1.053–1.281, p = 0.003) and modified Nutrition Risk in Critically Ill (mNUTRIC) score (HR:1.311, 95% CI:1.098–1.565, p = 0.003) were higher among those who needed PN. In the multivariate analysis, higher SOFA score (HR:1.221, 95% CI:1.057–1.410, p = 0.007) and higher triglyceride levels on ICU admission (HR:1.004, 95% CI:1.001–1.007, p = 0.003) were associated with an increased risk for the need of PN, whereas higher albumin levels on ICU admission (HR:0.424, 95% CI:0.210–0.687, p = 0.016) was associated with lower need of PN.

Conclusion

A higher SOFA and nutrition-related laboratory parameters on ICU admission may be associated with the need of PN after starting EN therapy. This may be related with a higher occurrence of GI complications, a feature of GI dysfunction.

Clinical trial registration

ClinicalTrials.gov: NCT03634943.
Pelvic PET/MR attenuation correction in the image space using deep learning
Authors: Abrahamsen Bendik Skarre , Knudtsen Ingerid Skjei , Eikenes Live , Bathen Tone Frost , Elschot Mattijs
Published: August 2023

Introduction

The five-class Dixon-based PET/MR attenuation correction (AC) model, which adds bone information to the four-class model by registering major bones from a bone atlas, has been shown to be error-prone. In this study, we introduce a novel method of accounting for bone in pelvic PET/MR AC by directly predicting the errors in the PET image space caused by the lack of bone in four-class Dixon-based attenuation correction.

Methods

A convolutional neural network was trained to predict the four-class AC error map relative to CT-based attenuation correction. Dixon MR images and the four-class attenuation correction µ-map were used as input to the models. CT and PET/MR examinations for 22 patients ([18F]FDG) were used for training and validation, and 17 patients were used for testing (6 [18F]PSMA-1007 and 11 [68Ga]Ga-PSMA-11). A quantitative analysis of PSMA uptake using voxel- and lesion-based error metrics was used to assess performance.

Results

In the voxel-based analysis, the proposed model reduced the median root mean squared percentage error from 12.1% and 8.6% for the four- and five-class Dixon-based AC methods, respectively, to 6.2%. The median absolute percentage error in the maximum standardized uptake value (SUVmax) in bone lesions improved from 20.0% and 7.0% for four- and five-class Dixon-based AC methods to 3.8%.

Conclusion

The proposed method reduces the voxel-based error and SUVmax errors in bone lesions when compared to the four- and five-class Dixon-based AC models.
Potential predictive role of gut microbiota to immunotherapy in HCC patients: a brief review
Authors: Muscolino Paola , Granata Barbara , Omero Fausto , De Pasquale Claudia , Campana Stefania , Calabrò Alessia , D’Anna Federica , Drommi Fabiana , Pezzino Gaetana , Cavaliere Riccardo , Ferlazzo Guido , Silvestris Nicola , Speranza Desirèe
Published: August 2023

The recent evolution of immunotherapy has revolutionised the treatment of hepatocellular carcinoma (HCC) and has led to new therapeutic standards. The advances in immunotherapy have been accompanied by the recognition of the role of the gut-liver axis in the progression of HCC but also of the clinical relevance of the gut microbiota, which influences host homeostasis but also cancer development and the response to treatment. Dysbiosis, by altering the tumour microenvironment, favours the activation of intracellular signalling pathways and promotes carcinogenesis. The gut microbiota, through their composition and immunomodulatory role, are thus strong predictors of the response to immune checkpoint inhibitor (ICI) treatment as well as an available target to improve ICI efficacy and reduce drug toxicities. In this review we examine the novel role of the gut microbiota as biomarkers in both the diagnosis of HCC and the clinical response to immunotherapy as well as its potential impact on clinical practice in the future.
Crescentic glomerulonephritis in children: short-term follow-up predicts long-term outcome
Authors: Zhang Pei , Yang Xiao , Gao Chun-lin , Wu Wei , Xia Zheng-kun
Published: August 2023

Background

Crescentic glomerulonephritis (CrGN) is a relatively rare but severe condition in childhood with the clinical feature of rapidly progressive glomerulonephritis (RPGN). The aim of this study is to investigate the clinicopathological features and prognosis of CrGN in children.

Methods

We retrospectively analyzed the clinical and laboratory data, renal pathological results, treatment, and outcome of 147 CrGN in two Chinese pediatric nephrology centers.

Results

Among the 147 children, there were 22 cases of type I (15.0%), 69 cases of type II (46.9%), and 56 cases of type III (38.1%). The mean percentages of crescents in CrGN I, II, and III were 85.3%, 68.7%, and 73.6%, respectively. The children with type I CrGN presented with more severe clinical manifestations and pathological lesions. The 3-month cumulative renal survival rates of types I, II, and III CrGN were 66.3%, 93.6%, and 75.6%, respectively. The 1-year cumulative renal survival rates of types I, II, and III CrGN were 56.9%, 85.3%, and 73.1%, respectively, and the 5-year cumulative renal survival rates of types I, II, and III CrGN were 33.8%, 73.5%, and 47.1%, respectively. The Kappa Consistency Test between the 3-month and 1-year total renal survival (82.1% vs. 74.7%) of the children was 0.683 (P < 0.001), and between the 1-year and 5-year total renal-free survival (78.3% vs. 69.1%) of the children was 0.476 (P < 0.001). The Bowman's Capsule Rupture (BCR), crescent, interstitial inflammation, and interstitial fibrosis/tubular atrophy (IF/TA) score were predictors of end-stage kidney disease (ESKD) risk but BCR showed better predictive value for ESKD than interstitial inflammation score (P = 0.027) and IF/TA score (P = 0.047).

Conclusion

Patients with type I tended to have the worst renal survival rates. The three-month renal prognosis could partially reflect the 1-year renal prognosis, and the 1-year mortality rate could partially reflect the 5-year mortality rate of children with CrGN.
Impact of in-utero electronic cigarette exposure on neonatal neuroinflammation, oxidative stress and mitochondrial function
Authors: Archie Sabrina Rahman , Sifat Ali Ehsan , Mara David , Ahn Yeseul , Akter Khondker Ayesha , Zhang Yong , Cucullo Luca , Abbruscato Thomas J.
Published: August 2023

Introduction: Despite the prevalence of the perception that electronic cigarettes (e-cig) are a safer alternative to tobacco smoke, growing concern about their potential toxic impact warrants adequate investigation focusing on special populations like maternal and pediatric groups. This study evaluated the consequences of maternal e-cig use on neonatal neuroinflammation, oxidative stress, and mitochondrial function in primary cultured neurons and postnatal day (PD) 7 and 90 brain.

Methodology: Pregnant CD1 mice were exposed to e‐cig vapor (2.4% nicotine) from gestational day 5 (E5) till PD7, and the primary neurons were isolated from pups at E16/17. Cellular total reactive oxygen species (ROS) and mitochondrial superoxide were measured in primary neurons using CM-H2DCFDA and Mitosox red, respectively. Mitochondrial function was assessed by Seahorse XF Cell Mitostress analysis. The level of pro-inflammatory cytokines was measured in primary neurons and PD7 and PD90 brains by RT-PCR and immunobead assay. Western blot analysis evaluated the expression of antioxidative markers (SOD-2, HO-1, NRF2, NQO1) and that of the proinflammatory modulator NF-κB.

Results: Significantly higher level of total cellular ROS (p < 0.05) and mitochondrial superoxide (p < 0.01) was observed in prenatally e-cig-exposed primary neurons. We also observed significantly reduced antioxidative marker expression and increased proinflammatory modulator and cytokines expression in primary neurons and PD7 (p < 0.05) but not in PD90 postnatal brain.

Conclusion: Our findings suggest that prenatal e-cig exposure induces postnatal neuroinflammation by promoting oxidative stress (OS), increasing cytokines’ levels, and disrupting mitochondrial function. These damaging events can alter the fetal brain’s immune functions, making such offspring more vulnerable to brain insults.
Efficacy and safety evaluation of Azvudine in the prospective treatment of COVID-19 based on four phase III clinical trials
Authors: Zhu Ke-Wei
Published: August 2023

Azvudine (FNC) is a synthetic nucleoside analog used to treat adult patients living with human immunodeficiency virus-1 (HIV-1) infection with high viral load. After phosphorylation, Azvudine inhibits RNA-dependent RNA polymerase, leading to the discontinuation of RNA chain synthesis in viruses. In addition, Azvudine is the first dual-target nucleoside oral drug worldwide to simultaneously target reverse transcriptase and viral infectivity factors in the treatment of HIV infection. On 9 August 2022, Azvudine was incorporated into the Guidelines for the Diagnosis and Treatment of Coronavirus Disease 2019 (version ninth) issued by the National Health Commission and the National Administration of Traditional Chinese Medicine. The recommended oral dose of Azvudine for the treatment of moderate coronavirus disease 2019 (COVID-19) is 5 mg once daily, and the duration of Azvudine treatment should not exceed 14 days. Four phase III clinical trials were performed during 2020–2022 to evaluate the efficacy and safety of Azvudine in the treatment of COVID-19. The results revealed that Azvudine could reduce nucleic acid-negative conversion time, viral load, and time to improvement in clinical conditions in patients with moderate COVID-19. In addition, Azvudine exhibited good safety and tolerance. Thereafter, Azvudine was incorporated into the Chinese guidelines and expert consensus for the treatment of COVID-19 and was highly approbated. Furthermore, Azvudine was also included in the Chinese guidelines for HIV infection.
Global carbon dioxide emissions analysis based on time series visualization
Authors: Liu Xuanyu , Zhao Meilin , Miao Qinghong
Published: August 2023

Carbon emissions and climate change is a significant challenge faced by the world today. The effects of climate change are felt globally, and many countries are making efforts to tackle this problem. The paper aims to analyze the carbon emissions data of 159 countries spanning from 1860 to 2016. By using the method of time-series visualization, the research aims to transform this data into complex networks to uncover any hidden information that might be useful in reducing emissions. By employing k-means, cluster analysis and other methods, we found that 1) While global carbon emissions continue to rise, there is a deceleration or reduction in carbon emissions in high-income countries. This deceleration or reduction may be attributed to factors such as policy measures and industrial shifts. 2) Additionally, our analysis highlights the significant increase in carbon emissions in certain economies that are not among the world’s leading nations in terms of overall economic size. This indicates the importance of these countries recognizing the relationship between carbon emissions and their economic development levels. 3) In addition, comparing the carbon emission levels of China and the United States, it can be found that even if the total amount of economic development is similar, their carbon emission patterns may be greatly different. Finally, we suggest that in the formulation of carbon emission policies, we should not only pay attention to the similar national models of economic development, but also take into account the actual development industry and stage of the country.
Beam test, simulation, and performance evaluation of PbF2 and PWO-UF crystals with SiPM readout for a semi-homogeneous calorimeter prototype with longitudinal segmentation
Authors: Cantone C. , Carsi S. , Ceravolo S. , Di Meco E. , Diociaiuti E. , Frank I. , Kholodenko S. , Martellotti S. , Mirra M. , Monti-Guarnieri P. , Moulson M. , Paesani D. , Prest M. , Romagnoni M. , Sarra I. , Sgarbossa F. , Soldani M. , Vallazza E.
Published: August 2023

Crilin (Crystal Calorimeter with Longitudinal Information) is a semi-homogeneous, longitudinally segmented electromagnetic calorimeter based on high-Z, ultra-fast crystals with UV-extended SiPM readout. The Crilin design has been proposed as a candidate solution for both a future Muon Collider barrel ECAL and for the Small Angle Calorimeter of the HIKE experiment. As a part of the Crilin development program, we have carried out beam tests of small (10 × 10 × 40 mm3) lead fluoride (PbF2) and ultra-fast lead tungstate (PbWO4, PWO-UF) crystals with 120 GeV electrons at the CERN SPS to study the light yield, timing response, and systematics of light collection with a proposed readout scheme. For a single crystal of PbF2, corresponding to a single Crilin cell, a time resolution of better than 25 ps is obtained for>3 GeV of deposited energy. For a single cell of PWO-UF, a time resolution of better than 45 ps is obtained for the same range of deposited energy. This timing performance fully satisfies the design requirements for the Muon Collider and HIKE experiments. Further optimizations of the readout scheme and crystal surface preparation are expected to bring further improvements.
Multi-scroll Hopfield neural network under electromagnetic radiation and its brain-like coupling synchronization
Authors: Fu Sen , Wang Xia , Gu Haiyang , Cao Xiaojing , Yao Zhengjun
Published: August 2023

Multi-scroll attractors have attracted attention because of their more complex topological structures and artificially controllable attractor structures. This paper proposes a new nonvolatile magnetic-controlled memristor and uses it to simulate the effect of membrane flux changes caused by neuronal exposure to electromagnetic radiation. A series of complex chaotic phenomena are found by plotting phase diagrams, bifurcation diagrams, attractor domains and 01 tests, including multi-scroll chaotic attractors controlled by memristors, symmetric bifurcation behavior, coexistence phenomena enhanced by initial offset. The mechanisms behind them are explained through equilibrium point analysis. A dual memristive HNN (MHNN) coupling synchronization model is proposed to simulate the synchronization between regions within the human brain. The Lyapunov function of the error is constructed to prove that this coupling synchronization scheme is ultimately bounded. The feasibility of this synchronization scheme is verified by establishing a Simulink model and conducting simulation experiments.
Identification of cytochrome P450 gene family and functional analysis of HgCYP33E1 from Heterodera glycines
Authors: You Jia , Chen Jingsheng , Hu Yanfeng , Wang Siru , Wang Jianli , Sun Tao , Shen Zhongbao
Published: August 2023

The cytochrome P450 (CYP) genes of nematode play a crucial role in the metabolic detoxification of xenobiotics including pesticides. Heterodera glycines, also known as the soybean cyst nematode, is a sedentary endoparasite that infests plant roots, causing high annual economic losses in soybean production regions globally. In this study, we identified 36 CYP genes at a genome-wide level of the H. glycines isolate TN10 using all CYPs from Caenorhabditis elegans as queries. Subsequently, a full-length cDNA of HgCYP33E1 which was significantly up-regulated by the conventional nematicide abamectin was initially cloned from H. glycines. It presented significantly higher expressions in the second-stage juvenile (J2) compared to other parasitic stages of H. glycines. qRT-PCR analysis suggested that the expression of HgCYP33E1 was also xenobiotically induced by soybean root exudate and the metabolites of biocontrol agents. Using RNA interference (RNAi), we investigated the function of HgCYP33E1 in H. glycines parasitism and nematicide selectivity. Compared to the control and dsGFP-treated group, silencing of HgCYP33E1 did not affect the J2 behaviors and the early invasion ability, while it decreased the number of J4s in soybean roots after 18-d inoculation with the dsHgCYP33E1-treated nematodes. In addition, knockdown of HgCYP33E1 in H. glycines resulted in an increase in J2 mortality after 24-h incubation with abamectin compared to the GFP dsRNA-soaked and the control group. These findings revealed the potential role of HgCYP33E1 in the xenobiotic detoxification pathway of H. glycines. Moreover, our data also provided valuable gene information for studying the functions of the CYP family in H. glycines host adaption.
Impact of drought on soil microbial biomass and extracellular enzyme activity
Authors: Qu Qing , Wang Zhen , Gan Quan , Liu Rentao , Xu Hongwei
Published: August 2023

Introduction

With the continuous changes in climate patterns due to global warming, drought has become an important limiting factor in the development of terrestrial ecosystems. However, a comprehensive understanding of the impact of drought on soil microbial activity at a global scale is lacking.

Methods

In this study, we aimed to examine the effects of drought on soil microbial biomass (carbon [MBC], nitrogen [MBN], and phosphorus [MBP]) and enzyme activity (β-1, 4-glucosidase [BG]; β-D-cellobiosidase [CBH]; β-1, 4-N-acetylglucosaminidase [NAG]; L-leucine aminopeptidase [LAP]; and acid phosphatase [AP]). Additionally, we conducted a meta-analysis to determine the degree to which these effects are regulated by vegetation type, drought intensity, drought duration, and mean annual temperature (MAT).

Result and discussion

Our results showed that drought significantly decreased the MBC, MBN, and MBP and the activity levels of BG and AP by 22.7%, 21.2%, 21.6%, 26.8%, and 16.1%, respectively. In terms of vegetation type, drought mainly affected the MBC and MBN in croplands and grasslands. Furthermore, the response ratio of BG, CBH, NAG, and LAP were negatively correlated with drought intensity, whereas MBN and MBP and the activity levels of BG and CBH were negatively correlated with drought duration. Additionally, the response ratio of BG and NAG were negatively correlated with MAT. In conclusion, drought significantly reduced soil microbial biomass and enzyme activity on a global scale. Our results highlight the strong impact of drought on soil microbial biomass and carbon- and phosphorus-acquiring enzyme activity.
Chloroplast magnesium transporters play essential but differential roles in maintaining magnesium homeostasis
Authors: Dukic Emilija , van Maldegem Kim A. , Shaikh Kashif Mohd , Fukuda Kento , Töpel Mats , Solymosi Katalin , Hellsten Jonna , Hansen Thomas Hesselhøj , Husted Søren , Higgins John , Sano Satoshi , Ishijima Sumio , Spetea Cornelia
Published: August 2023

Magnesium (Mg2+) is essential for photosynthesis in the chloroplasts of land plants and algae. Being the central ion of chlorophyll, cofactor and activator of many photosynthetic enzymes including RuBisCO, magnesium-deficient plants may suffer from leaf chlorosis symptoms and retarded growth. Therefore, the chloroplast Mg2+ concentration is tightly controlled by magnesium transport proteins. Recently, three different transporters from two distinct families have been identified in the chloroplast inner envelope of the model plant Arabidopsis thaliana: MGT10, MGR8, and MGR9. Here, we assess the individual roles of these three proteins in maintaining chloroplast Mg2+ homeostasis and regulating photosynthesis, and if their role is conserved in the model green alga Chlamydomonas reinhardtii. Phylogenetic analysis and heterologous expression revealed that the CorC-like MGR8 and MGR9 transport Mg2+ by a different mechanism than the CorA-like MGT10. MGR8 and MGT10 genes are highest expressed in leaves, indicating a function in chloroplast Mg2+ transport. MGR9 is important for chloroplast function and plant adaptation in conditions of deficiency or excess of Mg2+. Transmission electron microscopy indicated that MGT10 plays a differential role in thylakoid stacking than MGR8 and MGR9. Furthermore, we report that MGR8, MGR9, and MGT10 are involved in building up the pH gradient across the thylakoid membrane and activating photoprotection in conditions of excess light, however the mechanism has not been resolved yet. While there are no chloroplast MGR-like transporters in Chlamydomonas, we show that MRS4 is a homolog of MGT10, that is required for photosynthesis and cell growth. Taken together, our findings reveal that the studied Mg2+ transporters play essential but differential roles in maintaining chloroplast Mg2+ homeostasis.
WheatSpikeNet: an improved wheat spike segmentation model for accurate estimation from field imaging
Authors: Batin M. A. , Islam Muhaiminul , Hasan Md Mehedi , Azad AKM , Alyami Salem A. , Hossain Md Azam , Miklavcic Stanley J.
Published: August 2023

Phenotyping is used in plant breeding to identify genotypes with desirable characteristics, such as drought tolerance, disease resistance, and high-yield potentials. It may also be used to evaluate the effect of environmental circumstances, such as drought, heat, and salt, on plant growth and development. Wheat spike density measure is one of the most important agronomic factors relating to wheat phenotyping. Nonetheless, due to the diversity of wheat field environments, fast and accurate identification for counting wheat spikes remains one of the challenges. This study proposes a meticulously curated and annotated dataset, named as SPIKE-segm, taken from the publicly accessible SPIKE dataset, and an optimal instance segmentation approach named as WheatSpikeNet for segmenting and counting wheat spikes from field imagery. The proposed method is based on the well-known Cascade Mask RCNN architecture with model enhancements and hyperparameter tuning to provide state-of-the-art detection and segmentation performance. A comprehensive ablation analysis incorporating many architectural components of the model was performed to determine the most efficient version. In addition, the model’s hyperparameters were fine-tuned by conducting several empirical tests. ResNet50 with Deformable Convolution Network (DCN) as the backbone architecture for feature extraction, Generic RoI Extractor (GRoIE) for RoI pooling, and Side Aware Boundary Localization (SABL) for wheat spike localization comprises the final instance segmentation model. With bbox and mask mean average precision (mAP) scores of 0.9303 and 0.9416, respectively, on the test set, the proposed model achieved superior performance on the challenging SPIKE datasets. Furthermore, in comparison with other existing state-of-the-art methods, the proposed model achieved up to a 0.41% improvement of mAP in spike detection and a significant improvement of 3.46% of mAP in the segmentation tasks that will lead us to an appropriate yield estimation from wheat plants.
Pyramiding of transcription factor, PgHSF4, and stress-responsive genes of p68, Pg47, and PsAKR1 impart multiple abiotic stress tolerance in rice (Oryza sativa L.)
Authors: Sheela H. S. , Vennapusa Amaranatha R. , Melmaiee Kalpalatha , Prasad T. G. , Reddy Chandrashekar P.
Published: August 2023

Abiotic stresses such as drought, salinity, and heat stress significantly affect rice crop growth and production. Under uncertain climatic conditions, the concurrent multiple abiotic stresses at different stages of rice production became a major challenge for agriculture. Hence, improving rice’s multiple abiotic stress tolerance is essential to overcome unprecedented challenges under adverse environmental conditions. A significant challenge for rice breeding programs in improving abiotic stress tolerance involves multiple traits and their complexity. Multiple traits must be targeted to improve multiple stress tolerance in rice and uncover the mechanisms. With this hypothesis, in the present study gene stacking approach is used to integrate multiple traits involved in stress tolerance. The multigene transgenics co-expressing Pennisetum glaucum 47 (Pg47), Pea 68 (p68), Pennisetum glaucum Heat Shock Factor 4(PgHSF4), and Pseudomonas Aldo Keto Reductase 1 (PsAKR1) genes in the rice genotype (AC39020) were developed using the in-planta transformation method. The promising transgenic lines maintained higher yields under semi-irrigated aerobic cultivation (moisture stress). These 15 promising transgenic rice seedlings showed improved shoot and root growth traits under salinity, accelerating aging, temperature, and oxidative stress. They showed better physiological characteristics, such as chlorophyll content, membrane stability, and lower accumulation of reactive oxygen species, under multiple abiotic stresses than wild-type. Enhanced expression of transgenes and other stress-responsive downstream genes such as HSP70, SOD, APX, SOS, PP2C, and P5CS in transgenic lines suggest the possible molecular mechanism for imparting the abiotic stress tolerance. This study proved that multiple genes stacking as a novel strategy induce several mechanisms and responsible traits to overcome multiple abiotic stresses. This multigene combination can potentially improve tolerance to multiple abiotic stress conditions and pave the way for developing climate-resilient crops.
The influence of insecure attachment on undergraduates’ jealousy: the mediating effect of self-differentiation
Authors: Deng Min , Tadesse Endale , Khalid Sabika , Zhang Weida , Song Junrong , Gao Chunhai
Published: August 2023

Background

Jealousy is a complex emotion and can be healthy or pathological, depending on the intensity and the degree of control. Excessive jealousy was characterized by anxiety, anger, and alienation in the insecure attachment relationship.

Objective

To explore how insecure attachment triggered this intense emotion, this study investigated the relationship between two insecure attachment dimensions and jealousy and explored the influence of self-differentiation on the relationship.

Method

A total of 477 undergraduates participated in the study, and the Bringle self–report jealousy scale (BSJS), the relationship questionnaire (RQ), the intimate relationship experience questionnaire (ECR), and the revised edition of self–the differentiation questionnaire (DSR) were used.

Result

The results showed that: (1) attachment anxiety had a significant positive predictive effect on jealousy, but attachment avoidance had no significant positive predictive effect; (2) self-differentiation partially mediated the relationship between attachment anxiety and jealousy, but it has no significant mediating effect between attachment avoidance and jealousy.

Conclusion

The results suggest that attachment anxiety was correlated with jealousy because it strengthened the intensity of anxiety and anger toward their attachment figures and became out of control through a lower level of self-differentiation, which has important implications for clinical intervention.
Well-being and internal resources during the COVID-19 pandemic in relation to meaning in life and existential anxiety
Authors: David Laura Teodora , Truţa Camelia
Published: August 2023

The COVID-19 pandemic represents an event that placed humanity in a context where confrontation with uncertainty, isolation, life threats, and significant changes in one's life were on a scale that exceeded by far any previous individual or community crises. The interest of the present research was to investigate the relationship between meaning in life (MiL) and existential anxiety (EA) with personal internal resources such as creativity, playfulness, well-being, and personal meaning. A total of 451 participants from 48 countries (mean age 34.93 years, standard deviation 12.62, 31.9% men, 67.4% women) were questioned via online questionnaires between May and June 2020. Cluster analysis was performed on the meaning in life and existential anxiety that generated four categories of persons: Reactive, Superficial, Preoccupied, and Dedicated. Well-being and internal resources were associated mostly with the Dedicated type and less with the Reactive one. Arguments relying on the existential positive psychology suffering model and the hostile world scenario are presented to support the results and plead for interventions that elicit meaning, stimulate creativity, and guide people in finding purpose in order to ultimately promote psychological and mental health.
Effectiveness of attachment-based compassion therapy to reduce psychological distress in university students: a randomised controlled trial protocol
Authors: Beltrán-Ruiz María , Fernández Selene , García-Campayo Javier , Puebla-Guedea Marta , López-del-Hoyo Yolanda , Navarro-Gil Mayte , Montero-Marin Jesus
Published: August 2023

Introduction

Higher education, particularly university, is a challenge for many students that can lead to their mental health being seriously affected. The stress to which they are subject throughout their time at university can lead to anxiety and depression. “Third wave” psychotherapies, including compassion-based therapy, have been used to improve psychological outcomes, such as stress, anxiety, emotional distress and well-being. There are some signs that third wave psychotherapies reduce psychological distress in university students, but more and higher-quality studies are needed. In this randomised controlled trial (RCT), we hypothesise that the provision of attachment-based compassion therapy (ABCT) will be more effective than an active control group based on relaxation therapy for improving psychological distress in university students.

Methods and analysis

A two-arm RCT will be conducted involving 140 university undergraduate and postgraduate students from the University of Zaragoza and the National University of Distance Education (UNED) who reside in the autonomous community of Aragon, Spain. Interventions with either ABCT or relaxation therapy will be implemented, with an allocation ratio of 1:1 between groups. Both interventions will last six weeks and consist of six weekly group sessions lasting 1.5 h each. Data will be collected before and after the intervention, and there will be a follow-up at six months. The primary outcome will be psychological distress at post-intervention. Secondary outcomes will be depression, anxiety, stress and burnout symptoms, affectivity and emotional regulation. Attachment style, experiential avoidance, compassion (for others/oneself) and mindfulness skills will be measured as potential mechanistic variables. Intention-to-treat analysis will be performed using linear mixed regression models. The clinical significance of improvements will be calculated. Potential side effects will be monitored by an independent clinical psychologist.

Ethics and dissemination

This study was approved by the Clinical Research Ethics Committee of Aragón. Participant data will remain anonymous, and results will be submitted to peer-reviewed open-access journals and disseminated via conferences.

Clinical Trial Registration

ClinicalTrials.gov, identifier NCT05197595.
Does having more power make people more materialistic? The role of personal sense of power for gift preferences
Authors: Liang Shichang , Han Xiaoyan , Yuan Xueying , Liang Meiting , Zhang Yiwei , Liu Zhen , Xie Pin
Published: August 2023

Introduction

Gift-giving is a prevalent practice in daily life, with experiential gifts being identified in studies as having hedonic and interpersonal advantages, often yielding greater recipient satisfaction compared to material gifts. However, the reception of experiential gifts might not always align with expectations, as material gifts are valued for their enduring qualities. Thus, comprehending the contexts favoring material or experiential gift preferences becomes crucial.

Methods

Existing research primarily delves into external influences like income and social proximity, while intrinsic factors such as personal sense of power in interpersonal interactions have received limited attention. Guided by the Agentic-communal Model of Power, we conducted three studies to investigate how personal sense of power impact gift preferences.

Results

Our findings demonstrated that gift preferences are contingent upon personal sense of power. Specifically, those possessing a high personal sense of power exhibited a preference for material gifts over experiential ones, whereas individuals with a low personal sense of power favored experiential gifts over material ones. Further analysis revealed that the relationship between personal sense of power and gift preference is mediated by information processing fluency.

Discussion

This study contributes to the field of gift preferences and sheds light on the role of personal sense of power. By incorporating the Agentic-communal Model of Power, we offer novel insights into the dynamics between personal sense of power and gift preferences. These findings hold valuable implications for managerial strategies concerning gift selection and interpersonal interactions.