bluuebunny/crossref_metadata_2025 huggingface dataset:
Novel Heat-Integrated Hybrid Distillation and Adsorption Process for Coproduction of Cellulosic Ethanol, Heat, and Electricity from Actual Lignocellulosic Fermentation Broth
Authors: Nhien Le Cao , Van Duc Long Nguyen , Lee Moonyong
Published: June 2021

Cellulosic ethanol (CE) can not only be produced from a nonedible, cheap, and abundant lignocellulose feedstock but also can reduce carbon footprint significantly compared to starch ethanol. Despite great stimulation worldwide, CE production has not yet commercialized because of the complexity of lignocellulose. Therefore, intensive research and development are needed to improve CE technologies. In this study, a cost-efficient and sustainable design was proposed for the coproduction of CE, heat, and electricity from the actual lignocellulosic fermentation broth. First, a conventional coproduction process of CE, heat, and electricity based on hybrid distillation and adsorption (HDA) was simulated and optimized. Subsequently, various heat integrated (HI) techniques such as heat pump (HP), multi-effect distillation (MED), and combined HP-MED were evaluated to improve the CE process. The combined heat and power (CHP) process that utilized the combustible solids of the beer stillage was designed and integrated with the CE process. Structural alternatives were assessed for both economic and environmental impacts. The results show that the proposed HI-HDA process can save 36.9% and 33.6% of total annual costs and carbon footprint, respectively, compared to the conventional CE process. In the proposed HI-HDA coproduction process, the CE recovery process can be self-efficient in energy and the CHP can generate 12.0% more electricity than that in the conventional coproduction process.
Coordinating Control of an Offshore LVDC Microgrid Based Renewable Energy Resources for Voltage Regulation and Circulating Current Minimization
Authors: Nassar Walid , Anaya-Lara Olimpo , Ahmed Khaled
Published: June 2021

Multi-Use Platform (MUP) is a new topic addressed, extensively, under the EU funded projects that aim to exploit oceans in a sustainable way in order to reduce the costs of marine energy and to extract seafood. MUP’s electrical grid experiences many challenges, being offshore. One of these challenges is that only Alternating Current (AC) systems are considered which are inefficient, expensive and require bulky components. Considering the advantages of Direct Current (DC) systems, this paper aims to study the feasibility of using the DC system to improve the electrical infrastructure of the MUP’s grid. Floating energy unit comprising tidal, wind and solar energy resources is considered as a base unit for the MUP’s grid. The paper proposes a new distributed controller for grid voltage regulation and minimizing circulating current among parallel-connected floating energy units in an offshore Low Voltage Direct Current (LVDC) microgrid. A mathematical model is derived for n-parallel floating energy units with the proposed controller. Additionally, stability analysis for the overall model of a single floating energy unit is also presented. The analysis and simulation of the proposed DC system demonstrate that the system is stable and fault-rejected at different operating conditions.
Impact of Commercial Yeasts on Phenolic Profile of Plavac Mali Wines from Croatia
Authors: Jagatić Korenika Ana-Marija , Tomaz Ivana , Preiner Darko , Plichta Vedran , Jeromel Ana
Published: June 2021

Wine quality is influenced by the presence of over 500 different chemical compounds, with polyphenols having a crucial role in color intensity and tonality, astringency, mouthfeel, and overall impression formation, especially in red wine production. Their concentrations in wine can vary notably depending on the grape variety, the temperature and the length of maceration process, aging duration, and yeast selection. Therefore, in this work, the main goal was to determine the influence of five commercially available Saccharomyces yeasts provided from Lallemand, France and AEB, Italy, on the phenolic compound composition and chromatic parameters of Plavac mali wines produced from the grapes from coastal Dalmatia, grown at two different micro-locations. The achieved results pointed out the marked difference in individual polyphenol compound adsorption between tested yeasts. Fermol Super 16 was the one with the lowest and Lalvin D21 the strongest adsorption ability, regardless of vine growing location. These differences can be explained by the content of some anthocyanins (delphinidin and petunidin-3-O-glucoside) and gallic acid, and some flavan-3-ols. Tested strains also influenced wine color intensity, pointing out the possibility of modulating the style of a Plavac mali by the use of commercial yeasts.
Applying Pulsed Electric Fields to Whole Carrots Enhances the Bioaccessibility of Carotenoid and Phenolic Compounds in Derived Products
Authors: López-Gámez Gloria , Elez-Martínez Pedro , Martín-Belloso Olga , Soliva-Fortuny Robert
Published: June 2021

We propose the application of pulsed electric fields (PEF) to carrots to obtain derived products with increased phenolic and carotenoid bioaccessibility. For this purpose, juices, purees, and oil-added purees were obtained from whole PEF-treated carrots (five pulses of 3.5 kV cm−1; 0.61 kJ kg−1). In order to obtain shelf-stable products, the effect of a thermal treatment (70 °C for 10 min) was also studied. Carrot juices exhibited the highest carotenoid (43.4 mg/100 g fresh weight) and phenolic (322 mg kg−1 dry weight) contents. However, caffeic and coumaric acid derivatives were highly sensitive to PEF. The phenolic bioaccessibility reached 100% in purees obtained from the PEF-treated carrots, whereas the further thermally treated oil-added purees exhibited the greatest carotenoid bioaccessibility (7.8%). The increase in carotenoid bioaccessibility could be related to their better release and solubilization into micelles. The results suggest that food matrix aspects apart from particle size (e.g., pectin characteristics) are involved in phenolic bioaccessibility.
Lateral Root Initiation and the Analysis of Gene Function Using Genome Editing with CRISPR in Arabidopsis
Authors: Vangheluwe Nick , Beeckman Tom
Published: June 2021

Lateral root initiation is a post-embryonic process that requires the specification of a subset of pericycle cells adjacent to the xylem pole in the primary root into lateral root founder cells. The first visible event of lateral root initiation in Arabidopsis is the simultaneous migration of nuclei in neighbouring founder cells. Coinciding cell cycle activation is essential for founder cells in the pericycle to undergo formative divisions, resulting in the development of a lateral root primordium (LRP). The plant signalling molecule, auxin, is a major regulator of lateral root development; the understanding of the molecular mechanisms controlling lateral root initiation has progressed tremendously by the use of the Arabidopsis model and a continual improvement of molecular methodologies. Here, we provide an overview of the visible events, cell cycle regulators, and auxin signalling cascades related to the initiation of a new LRP. Furthermore, we highlight the potential of genome editing technology to analyse gene function in lateral root initiation, which provides an excellent model to answer fundamental developmental questions such as coordinated cell division, growth axis establishment as well as the specification of cell fate and cell polarity.
Virtual Reality Therapy for Depression and Mood in Long-Term Care Facilities
Authors: Zhai Kevin , Dilawar Azwa , Yousef Mohammad S. , Holroyd Sean , El-Hammali Haithem , Abdelmonem Marwa
Published: June 2021

Virtual reality (VR) describes a family of technologies which immerse users in sensorily-stimulating virtual environments. Such technologies have increasingly found applications in the treatment of neurological and mental health disorders. Depression, anxiety, and other mood abnormalities are of concern in the growing older population—especially those who reside in long-term care facilities (LTCFs). The transition from the familiar home environment to the foreign LTCF introduces a number of stressors that can precipitate depression. However, recent studies reveal that VR therapy (VRT) can promote positive emotionality and improve cognitive abilities in older people, both at home and in LTCFs. VR thus holds potential in allowing older individuals to gradually adapt to their new environments—thereby mitigating the detrimental effects of place attachment and social exclusion. Nevertheless, while the current psychological literature is promising, the implementation of VR in LTCFs faces many challenges. LTCF residents must gain trust in VR technologies, care providers require training to maximize the positive effects of VRT, and decision makers must evaluate both the opportunities and obstacles in adopting VR. In this review article, we concisely discuss the implications of depression related to place attachment in LTCFs, and explore the potential therapeutic applications of VR.
Long Non-Coding RNA Epigenetics
Authors: Kazimierczyk Marek , Wrzesinski Jan
Published: June 2021

Long noncoding RNAs exceeding a length of 200 nucleotides play an important role in ensuring cell functions and proper organism development by interacting with cellular compounds such as miRNA, mRNA, DNA and proteins. However, there is an additional level of lncRNA regulation, called lncRNA epigenetics, in gene expression control. In this review, we describe the most common modified nucleosides found in lncRNA, 6-methyladenosine, 5-methylcytidine, pseudouridine and inosine. The biosynthetic pathways of these nucleosides modified by the writer, eraser and reader enzymes are important to understanding these processes. The characteristics of the individual methylases, pseudouridine synthases and adenine–inosine editing enzymes and the methods of lncRNA epigenetics for the detection of modified nucleosides, as well as the advantages and disadvantages of these methods, are discussed in detail. The final sections are devoted to the role of modifications in the most abundant lncRNAs and their functions in pathogenic processes.
MYC-Induced Replicative Stress: A Double-Edged Sword for Cancer Development and Treatment
Authors: Curti Laura , Campaner Stefano
Published: June 2021

MYC is a transcription factor that controls the expression of a large fraction of cellular genes linked to cell cycle progression, metabolism and differentiation. MYC deregulation in tumors leads to its pervasive genome-wide binding of both promoters and distal regulatory regions, associated with selective transcriptional control of a large fraction of cellular genes. This pairs with alterations of cell cycle control which drive anticipated S-phase entry and reshape the DNA-replication landscape. Under these circumstances, the fine tuning of DNA replication and transcription becomes critical and may pose an intrinsic liability in MYC-overexpressing cancer cells. Here, we will review the current understanding of how MYC controls DNA and RNA synthesis, discuss evidence of replicative and transcriptional stress induced by MYC and summarize preclinical data supporting the therapeutic potential of triggering replicative stress in MYC-driven tumors.
The Antimicrobial Activity of Peripheral Blood Neutrophils Is Altered in Patients with Primary Ciliary Dyskinesia
Authors: Cockx Maaike , Blanter Marfa , Gouwy Mieke , Ruytinx Pieter , Abouelasrar Salama Sara , Knoops Sofie , Pörtner Noëmie , Vanbrabant Lotte , Lorent Natalie , Boon Mieke , Struyf Sofie
Published: June 2021

The airways of patients with primary ciliary dyskinesia (PCD) contain persistently elevated neutrophil numbers and CXCL8 levels. Despite their abundance, neutrophils fail to clear the airways from bacterial infections. We investigated whether neutrophil functions are altered in patients with PCD. Neutrophils from patients and healthy controls (HC) were isolated from peripheral blood and exposed to various bacterial stimuli or cytokines. Neutrophils from patients with PCD were less responsive to low levels of fMLF in three different chemotaxis assays (p < 0.05), but expression of the fMLF receptors was unaltered. PCD neutrophils showed normal phagocytic function and expression of adhesion molecules. However, PCD neutrophils produced less reactive oxygen species upon stimulation with bacterial products or cytokines compared to HC neutrophils (p < 0.05). Finally, the capacity to release DNA, as observed during neutrophil extracellular trap formation, seemed to be reduced in patients with PCD compared to HC (p = 0.066). These results suggest that peripheral blood neutrophils from patients with PCD, in contrast to those of patients with cystic fibrosis or COPD, do not show features of over-activation, neither on baseline nor after stimulation. If these findings extend to lung-resident neutrophils, the reduced neutrophil activity could possibly contribute to the recurrent respiratory infections in patients with PCD.
Regulation of Cancer Metabolism by Deubiquitinating Enzymes: The Warburg Effect
Authors: Kim So-Hee , Baek Kwang-Hyun
Published: June 2021

Cancer is a disorder of cell growth and proliferation, characterized by different metabolic pathways within normal cells. The Warburg effect is a major metabolic process in cancer cells that affects the cellular responses, such as proliferation and apoptosis. Various signaling factors down/upregulate factors of the glycolysis pathway in cancer cells, and these signaling factors are ubiquitinated/deubiquitinated via the ubiquitin–proteasome system (UPS). Depending on the target protein, DUBs act as both an oncoprotein and a tumor suppressor. Since the degradation of tumor suppressors and stabilization of oncoproteins by either negative regulation by E3 ligases or positive regulation of DUBs, respectively, promote tumorigenesis, it is necessary to suppress these DUBs by applying appropriate inhibitors or small molecules. Therefore, we propose that the DUBs and their inhibitors related to the Warburg effect are potential anticancer targets.
Proteomic Profiling of BRAFV600E Mutant Colon Cancer Cells Reveals the Involvement of Nucleophosmin/c-Myc Axis in Modulating the Response and Resistance to BRAF Inhibition by Vemurafenib
Authors: Grbčić Petra , Fučkar Čupić Dora , Gamberi Tania , Kraljević Pavelić Sandra , Sedić Mirela
Published: June 2021

BRAFV600E mutations are found in approximately 10% of colorectal cancer patients and are associated with worse prognosis and poor outcomes with systemic therapies. The aim of this study was to identify novel druggable features of BRAFV600E-mutated colon cancer (CC) cells associated with the response and resistance to BRAFV600E inhibitor vemurafenib. Towards this aim, we carried out global proteomic profiling of BRAFV600E mutant vs. KRAS mutant/BRAF wild-type and double wild-type KRAS/BRAF CC cells followed by bioinformatics analyses. Validation of selected proteomic features was performed by immunohistochemistry and in silico using the TCGA database. We reveal an increased abundance and activity of nucleophosmin (NPM1) in BRAFV600E-mutated CC in vitro, in silico and in tumor tissues from colon adenocarcinoma patients and demonstrate the roles of NPM1 and its interaction partner c-Myc in conveying the resistance to vemurafenib. Pharmacological inhibition of NPM1 effectively restored the sensitivity of vemurafenib-resistant BRAF-mutated CC cells by down-regulating c-Myc expression and activity and consequently suppressing its transcriptional targets RanBP1 and phosphoserine phosphatase that regulate centrosome duplication and serine biosynthesis, respectively. Altogether, findings from this study suggest that the NPM1/c-Myc axis could represent a promising therapeutic target to thwart resistance to vemurafenib in BRAF-mutated CC.
Erratum: Yamazaki et al. Editing DNA Methylation in Mammalian Embryos. Int. J. Mol. Sci. 2020, 21, 637
Authors: Yamazaki Taiga , Hatano Yu , Taniguchi Ryoya , Kobayashi Noritada , Yamagata Kazuo
Published: June 2021

The authors wish to make the following corrections to our previously published paper [...]
The Effectiveness of Glutathione Redox Status as a Possible Tumor Marker in Colorectal Cancer
Authors: Acevedo-León Delia , Monzó-Beltrán Lidia , Gómez-Abril Segundo Ángel , Estañ-Capell Nuria , Camarasa-Lillo Natalia , Pérez-Ebri Marisa Luisa , Escandón-Álvarez Jorge , Alonso-Iglesias Eulalia , Santaolaria-Ayora Marisa Luisa , Carbonell-Moncho Araceli , Ventura-Gayete Josep , Pla Luis , Martínez-Bisbal Maria Carmen , Martínez-Máñez Ramón , Bagán-Debón Leticia , Viña-Almunia Aurora , Martínez-Santamaría M. Amparo , Ruiz-Luque María , Alonso-Fernández Jorge , Bañuls Celia , Sáez Guillermo
Published: June 2021

The role of oxidative stress (OS) in cancer is a matter of great interest due to the implication of reactive oxygen species (ROS) and their oxidation products in the initiation of tumorigenesis, its progression, and metastatic dissemination. Great efforts have been made to identify the mechanisms of ROS-induced carcinogenesis; however, the validation of OS byproducts as potential tumor markers (TMs) remains to be established. This interventional study included a total of 80 colorectal cancer (CRC) patients and 60 controls. By measuring reduced glutathione (GSH), its oxidized form (GSSG), and the glutathione redox state in terms of the GSSG/GSH ratio in the serum of CRC patients, we identified significant changes as compared to healthy subjects. These findings are compatible with the effectiveness of glutathione as a TM. The thiol redox state showed a significant increase towards oxidation in the CRC group and correlated significantly with both the tumor state and the clinical evolution. The sensitivity and specificity of serum glutathione levels are far above those of the classical TMs CEA and CA19.9. We conclude that the GSSG/GSH ratio is a simple assay which could be validated as a novel clinical TM for the diagnosis and monitoring of CRC.
New Insights of SF1 Neurons in Hypothalamic Regulation of Obesity and Diabetes
Authors: Fosch Anna , Zagmutt Sebastián , Casals Núria , Rodríguez-Rodríguez Rosalía
Published: June 2021

Despite the substantial role played by the hypothalamus in the regulation of energy balance and glucose homeostasis, the exact mechanisms and neuronal circuits underlying this regulation remain poorly understood. In the last 15 years, investigations using transgenic models, optogenetic, and chemogenetic approaches have revealed that SF1 neurons in the ventromedial hypothalamus are a specific lead in the brain’s ability to sense glucose levels and conduct insulin and leptin signaling in energy expenditure and glucose homeostasis, with minor feeding control. Deletion of hormonal receptors, nutritional sensors, or synaptic receptors in SF1 neurons triggers metabolic alterations mostly appreciated under high-fat feeding, indicating that SF1 neurons are particularly important for metabolic adaptation in the early stages of obesity. Although these studies have provided exciting insight into the implications of hypothalamic SF1 neurons on whole-body energy homeostasis, new questions have arisen from these results. Particularly, the existence of neuronal sub-populations of SF1 neurons and the intricate neurocircuitry linking these neurons with other nuclei and with the periphery. In this review, we address the most relevant studies carried out in SF1 neurons to date, to provide a global view of the central role played by these neurons in the pathogenesis of obesity and diabetes.
Cellular Chaperone Function of Intrinsically Disordered Dehydrin ERD14
Authors: Murvai Nikoletta , Kalmar Lajos , Szabo Beata , Schad Eva , Micsonai András , Kardos József , Buday László , Han Kyou-Hoon , Tompa Peter , Tantos Agnes
Published: June 2021

Disordered plant chaperones play key roles in helping plants survive in harsh conditions, and they are indispensable for seeds to remain viable. Aside from well-known and thoroughly characterized globular chaperone proteins, there are a number of intrinsically disordered proteins (IDPs) that can also serve as highly effective protecting agents in the cells. One of the largest groups of disordered chaperones is the group of dehydrins, proteins that are expressed at high levels under different abiotic stress conditions, such as drought, high temperature, or osmotic stress. Dehydrins are characterized by the presence of different conserved sequence motifs that also serve as the basis for their categorization. Despite their accepted importance, the exact role and relevance of the conserved regions have not yet been formally addressed. Here, we explored the involvement of each conserved segment in the protective function of the intrinsically disordered stress protein (IDSP) A. thaliana’s Early Response to Dehydration (ERD14). We show that segments that are directly involved in partner binding, and others that are not, are equally necessary for proper function and that cellular protection emerges from the balanced interplay of different regions of ERD14.
Stress Modifies the Expression of Glucocorticoid-Responsive Genes by Acting at Epigenetic Levels in the Rat Prefrontal Cortex: Modulatory Activity of Lurasidone
Authors: Brivio Paola , Sbrini Giulia , Tarantini Letizia , Parravicini Chiara , Gruca Piotr , Lason Magdalena , Litwa Ewa , Favero Chiara , Riva Marco Andrea , Eberini Ivano , Papp Mariusz , Bollati Valentina , Calabrese Francesca
Published: June 2021

Epigenetics is one of the mechanisms by which environmental factors can alter brain function and may contribute to central nervous system disorders. Alterations of DNA methylation and miRNA expression can induce long-lasting changes in neurobiological processes. Hence, we investigated the effect of chronic stress, by employing the chronic mild stress (CMS) and the chronic restraint stress protocol, in adult male rats, on the glucocorticoid receptor (GR) function. We focused on DNA methylation specifically in the proximity of the glucocorticoid responsive element (GRE) of the GR responsive genes Gadd45β, Sgk1, and Gilz and on selected miRNA targeting these genes. Moreover, we assessed the role of the antipsychotic lurasidone in modulating these alterations. Chronic stress downregulated Gadd45β and Gilz gene expression and lurasidone normalized the Gadd45β modification. At the epigenetic level, CMS induced hypermethylation of the GRE of Gadd45β gene, an effect prevented by lurasidone treatment. These stress-induced alterations were still present even after a period of rest from stress, indicating the enduring nature of such changes. However, the contribution of miRNA to the alterations in gene expression was moderate in our experimental conditions. Our results demonstrated that chronic stress mainly affects Gadd45β expression and methylation, effects that are prolonged over time, suggesting that stress leads to changes in DNA methylation that last also after the cessation of stress procedure, and that lurasidone is a modifier of such mechanisms.
Silica Particles Trigger the Exopolysaccharide Production of Harsh Environment Isolates of Growth-Promoting Rhizobacteria and Increase Their Ability to Enhance Wheat Biomass in Drought-Stressed Soils
Authors: Fetsiukh Anastasiia , Conrad Julian , Bergquist Jonas , Timmusk Salme
Published: June 2021

In coming decades, drought is expected to expand globally owing to increased evaporation and reduced rainfall. Understanding, predicting, and controlling crop plants’ rhizosphere has the potential to manipulate its responses to environmental stress. Our plant growth-promoting rhizobacteria (PGPR) are isolated from a natural laboratory, ‘The Evolution Canyon’, Israel, (EC), from the wild progenitors of cereals, where they have been co-habituating with their hosts for long periods of time. The study revealed that commercial TM50 silica particles (SN) triggered the PGPR production of exopolysaccharides (EPS) containing D-glucuronate (D-GA). The increased EPS content increased the PGPR water-holding capacity (WHC) and osmotic pressure of the biofilm matrix, which led to enhanced plant biomass in drought-stressed growth environments. Light- and cryo-electron- microscopic studies showed that, in the presence of silica (SN) particles, bacterial morphology is changed, indicating that SNs are associated with significant reprogramming in bacteria. The findings encourage the development of large-scale methods for isolate formulation with natural silicas that ensure higher WHC and hyperosmolarity under field conditions. Osmotic pressure involvement of holobiont cohabitation is also discussed.
Comparative Study of Metagenomics and Metatranscriptomics to Reveal Microbiomes in Overwintering Pepper Fruits
Authors: Jo Yeonhwa , Back Chang-Gi , Kim Kook-Hyung , Chu Hyosub , Lee Jeong Hun , Moh Sang Hyun , Cho Won Kyong
Published: June 2021

Red pepper (Capsicum annuum, L.), is one of the most important spice plants in Korea. Overwintering pepper fruits are a reservoir of various microbial pepper diseases. Here, we conducted metagenomics (DNA sequencing) and metatranscriptomics (RNA sequencing) using samples collected from three different fields. We compared two different library types and three different analytical methods for the identification of microbiomes in overwintering pepper fruits. Our results demonstrated that DNA sequencing might be useful for the identification of bacteria and DNA viruses such as bacteriophages, while mRNA sequencing might be beneficial for the identification of fungi and RNA viruses. Among three analytical methods, KRAKEN2 with raw data reads (KRAKEN2_R) might be superior for the identification of microbial species to other analytical methods. However, some microbial species with a low number of reads were wrongly assigned at the species level by KRAKEN2_R. Moreover, we found that the databases for bacteria and viruses were better established as compared to the fungal database with limited genome data. In summary, we carefully suggest that different library types and analytical methods with proper databases should be applied for the purpose of microbiome study.
A Comparative Study of Biocompatibility in Rat Connective Tissue of a New Mineral Trioxide Compound (Theracal) versus MTA and a Bioactive G3 Glass
Authors: Mena-Álvarez Jesús , Rico-Romano Cristina , Gutiérrez-Ortega Carlos , Arias-Sanz Pablo , Castro-Urda Javier
Published: June 2021

The aim of this paper was to assess the differences in tissue response to implantation during 15, 30 and 45 days in the subcutaneous connective tissue of Wistar rats from three biomaterials: Angelus MTA®, Theracal LC®, and Angelus MTA® to which 25% bioglass G3 was added. Twenty-four Wistar rats were used, the materials were inserted into the rat’s dorsal area in silicone tubes 5 mm long by 1.5 mm diameter. Histological reaction was assessed at 15, 30, and 45 days after implantation. They were then stained with hematoxylin eosin and evaluated by two observers. Data were analyzed using Fisher’s exact test and Mann–Whitney’s U test was used to determine the association between variables. Angelus MTA induced the formation of dystrophic calcifications twice as much as Theracal LC (p < 0.05). The addition of G3 did not affect the greater or lesser occurrence of calcifications (p > 0.05). Theracal LC and MTA plus G3 caused an inflammatory reaction, which was chronic at 15 days and decreased in intensity, almost disappearing after 45 days. Theracal LC, as well as Angelus MTA plus G3, were well tolerated when implanted in the subcutaneous connective tissue of rat. Histologically, no inconvenience was found for the use by direct contact of Theracal LC, and the mixture of MTA with 25% bioactive glass G3, in the tissue of Wistar rats.
Detection of Psychopathic Traits in Emotional Faces
Authors: Olderbak Sally , Bader Christina , Hauser Nicole , Kleitman Sabina
Published: June 2021

When meeting someone at zero acquaintance, we make assumptions about each other that encompass emotional states, personality traits, and even cognitive abilities. Evidence suggests individuals can accurately detect psychopathic personality traits in strangers based on short video clips or photographs of faces. We present an in-depth examination of this ability. In two studies, we investigated whether high psychopathy traits are perceivable and whether other traits affect ratings of psychopathic traits in the sense of a halo effect. On the perceiver’s end, we additionally examined how cognitive abilities and personality traits of the responders affect these ratings. In two studies (n1 = 170 community adults from the USA, n2 = 126 students from Australia), participants rated several targets on several characteristics of psychopathy, as well as on attractiveness, masculinity, sympathy, trustworthiness, neuroticism, intelligence, and extraversion. Results show that responders were generally able to detect psychopathy. Responders generally came to a consensus in their ratings, and using profile similarity metrics, we found a weak relation between ratings of psychopathy and the targets’ psychopathy level as determined by the Psychopathy Checklist: Short Version. Trait ratings, though, were influenced by the ratings of other traits like attractiveness. Finally, we found accuracy in the perception of psychopathy was positively related to fluid intelligence but unrelated to emotion perception ability.
A 1.02 μW Autarkic Threshold-Based Sensing and Energy Harvesting Interface Using a Single Piezoelectric Element
Authors: Agorastou Zoi , Kalenteridis Vasileios , Siskos Stylianos
Published: June 2021

A self-powered piezoelectric sensor interface employing part of the signal that is not intended for measurement to sustain its autonomous operation was designed using XH018 (180 nm) technology. The aim of the proposed circuit, besides the energy self-sufficiency of the sensor, is to provide an interface that eliminates the effect of the harvesting process on the piezoelectric output signal which contains context data. This is achieved by isolating part of the signal that is desirable for sensing from the harvesting process so that the former is not affected or distorted by the latter. Moreover, the circuit manages to self-start its operation, so no additional battery or pre-charged capacitor is needed. The circuit achieves a very low power consumption of 1.02 μW. As a proof of concept, the proposed interfacing circuit is implemented in order to be potentially used for weigh-in-motion applications.
Non-Small Cell Lung Cancer Harboring Concurrent EGFR Genomic Alterations: A Systematic Review and Critical Appraisal of the Double Dilemma
Authors: Gristina Valerio , La Mantia Maria , Galvano Antonio , Cutaia Sofia , Barraco Nadia , Castiglia Marta , Perez Alessandro , Bono Marco , Iacono Federica , Greco Martina , Calcara Katia , Calò Valentina , Rizzo Sergio , Incorvaia Lorena , Lisanti Maria Chiara , Santanelli Giulia , Sardo Delia , Inguglia Sara , Insalaco Lavinia , Castellana Luisa , Cusenza Stefania , Pantuso Gianni , Russo Antonio , Bazan Viviana
Published: June 2021

The molecular pathways which promote lung cancer cell features have been broadly explored, leading to significant improvement in prognostic and diagnostic strategies. Epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors (TKIs) have dramatically altered the treatment approach for patients with metastatic non-small cell lung cancer (NSCLC). Latest investigations by using next-generation sequencing (NGS) have shown that other oncogenic driver mutations, believed mutually exclusive for decades, could coexist in EGFR-mutated NSCLC patients. However, the exact clinical and pathological role of concomitant genomic aberrations needs to be investigated. In this systematic review, we aimed to summarize the recent data on the oncogenic role of concurrent genomic alterations, by specifically evaluating the characteristics, the pathological significance, and their potential impact on the treatment approach.
Attribution Analysis of Climate and Anthropic Factors on Runoff and Vegetation Changes in the Source Area of the Yangtze River from 1982 to 2016
Authors: Ji Guangxing , Song Huiyun , Wei Hejie , Wu Leying
Published: June 2021

Analyzing the temporal variation of runoff and vegetation and quantifying the impact of anthropic factors and climate change on vegetation and runoff variation in the source area of the Yangtze River (SAYR), is of great significance for the scientific response to the ecological protection of the region. Therefore, the Budyko hypothesis method and multiple linear regression method were used to quantitatively calculate the contribution rates of climate change and anthropic factors to runoff and vegetation change in the SAYR. It was found that: (1) The runoff, NDVI, precipitation, and potential evaporation in the SAYR from 1982 to 2016 all showed an increasing trend. (2) The mutation year of runoff data from 1982 to 2016 in the SAYR is 2004, and the mutation year of NDVI data from 1982 to 2016 in the SAYR is 1998. (3) The contribution rates of precipitation, potential evaporation and anthropic factors to runoff change of the SAYR are 75.98%, −9.35%, and 33.37%, respectively. (4) The contribution rates of climatic factors and anthropic factors to vegetation change of the SAYR are 38.56% and 61.44%, respectively.
Smart Mobile Application for Short-Haul Cargo Transportation
Authors: Azab Ahmed , Park Jaehyun , Mostafa Noha A.
Published: June 2021

Smart service systems have been dealing with various wicked social and cultural problems by providing diverse solutions for society. Cargo transportation is one of the most challenging issues in logistics; in this paper, we explore multiple stakeholders’ information environments surrounding containerized cargo transportation by a design thinking approach. Throughout the analysis, the latent problems are summarized in the following two manners: (1) miscommunication with inefficient information flow among multiple stakeholders; and (2) a lack of resource management coordination among service providers. With these two identified problems, the objective of this work is to develop a prototype information and communication technology-based service application for both trucking companies and customers. This work makes two contributions. First, it methodologically proposes a customer-centered design approach for logistics management by adopting a design thinking approach. Second, it suggests a practical ICT-based solution for the mentioned problems.
Learning Adaptive Coarse Spaces of BDDC Algorithms for Stochastic Elliptic Problems with Oscillatory and High Contrast Coefficients
Authors: Chung Eric , Kim Hyea-Hyun , Lam Ming-Fai , Zhao Lina
Published: June 2021

In this paper, we consider the balancing domain decomposition by constraints (BDDC) algorithm with adaptive coarse spaces for a class of stochastic elliptic problems. The key ingredient in the construction of the coarse space is the solutions of local spectral problems, which depend on the coefficient of the PDE. This poses a significant challenge for stochastic coefficients as it is computationally expensive to solve the local spectral problems for every realization of the coefficient. To tackle this computational burden, we propose a machine learning approach. Our method is based on the use of a deep neural network (DNN) to approximate the relation between the stochastic coefficients and the coarse spaces. For the input of the DNN, we apply the Karhunen–Loève expansion and use the first few dominant terms in the expansion. The output of the DNN is the resulting coarse space, which is then applied with the standard adaptive BDDC algorithm. We will present some numerical results with oscillatory and high contrast coefficients to show the efficiency and robustness of the proposed scheme.
Misleading HbA1c Measurement in Diabetic Patients with Hemoglobin Variants
Authors: Mitchai Manthana , Suwansaksri Nattakarn , Seanseeha Suphakdee , Saenboonsiri Jindamanee , Kraitree Putthichai , Piyapromdee Jirasak , Silsirivanit Atit
Published: June 2021

Background and Objectives: Hemoglobin A1c (HbA1c) is widely used for the monitoring and management of diabetes mellitus. The aim of this study is to investigate the influence of hemoglobin (Hb) variants on the measurement of HbA1c. Materials and Methods: HbA1c levels of 845 blood samples obtained from diabetic patients with various hemoglobin types were measured using a turbidimetric inhibition immunoassay and capillary electrophoresis. Results: Of 845 patients with diabetes, 65.7% (555/845) have the normal hemoglobin type (A2A) and 34.3% (290/845) have various abnormal hemoglobin types, including heterozygous HbE 30.2% (255/845), homozygous HbE 1.9 % (16/845), Hb Constant Spring (CS) trait 1.4% (12/845), CSEA Bart’s 0.2% (2/845), and beta-thalassemia trait 0.6% (5/845). In most of the patients with diabetes, HbA1c levels determined by two different methods, inhibition immunoassay and capillary electrophoresis, gave strong positive correlation (R = 0.901, P < 0.001), except for those with homozygous HbE (N = 16) and CSEA Bart’s (N = 2). In all 18 patients with homozygous HbE and CSEA Bart’s, the HbA1c was undetectable by capillary electrophoresis, meaning that their estimated average glucose was undeterminable, although their HbA1c levels could be measured using an inhibition immunoassay. The discrepancy of HbA1c results obtained from two different methods is noted in patients without HbA. Conclusions: We have demonstrated the erroneous nature of HbA1c measurement in patients with hemoglobin variants, especially in those without HbA expression. Therefore, in the population with a high prevalence of hemoglobinopathies, hemoglobin typing should be considered as basic information prior to HbA1c measurement.
Multidrug Resistance Like Protein 1 Activity in Malpighian Tubules Regulates Lipid Homeostasis in Drosophila
Authors: Liu Wen , Cao Hao , Kimari Moses , Maronitis Georgios , Williams Michael J. , Schiöth Helgi B
Published: June 2021

Multidrug resistance proteins (MRPs), members of the ATP-binding cassette transporter (ABC transporter) family, are pivotal for transporting endo- and xenobiotics, which confer resistance to anticancer agents and contribute to the clearance of oxidative products. However, their function in many biological processes is still unclear. We investigated the role of an evolutionarily conserved MRP in metabolic homeostasis by knocking down the expression of Drosophila multidrug-resistance like protein 1 (MRP) in several tissues involved in regulating metabolism, including the gut, fat body, and Malpighian tubules. Interestingly, only suppression of MRP in the Malpighian tubules, the functional equivalent to the human kidney, was sufficient to cause abnormal lipid accumulation and disrupt feeding behavior. Furthermore, reduced Malpighian tubule MRP expression resulted in increased Hr96 (homolog of human pregnane X receptor) expression. Hr96 is known to play a role in detoxification and lipid metabolism processes. Reduced expression of MRP in the Malpighian tubules also conveyed resistance to oxidative stress, as well as reduced normal levels of reactive oxygen species in adult flies. This study reveals that an evolutionarily conserved MRP is required in Drosophila Malpighian tubules for proper metabolic homeostasis.
Progress of Flake Powder Metallurgy Research
Authors: Sadeghi Behzad , Cavaliere Pasquale
Published: June 2021

This paper reviewed several recent progresses of the new powder metallurgy technology known as flake powder metallurgy (FPM) including different processing routes, conventional FPM (C-FPM), slurry blending (SB), shift-speed ball milling (SSBM), and high-shear pre-dispersion and SSBM (HSPD/SSBM). The name of FPM was derived from the use of flake metal powders obtained by low-speed ball milling (LSBM) from spherical powder. In this case, the uniformity of reinforcement distribution leads to increased strength and ductility. Powder is the basic unit in PM, especially advanced PM, and its control is key to various new PM technologies. The FPM is a typical method for finely controlling the powder shape through low-energy ball milling (LEBM) to realize the preparation of advanced material structures. The present paper represents a review of the main results of research on FPM and indicates the potential for future studies devoted to the optimization of this processing route.
Directed Energy Deposition of AISI 316L Stainless Steel Powder: Effect of Process Parameters
Authors: Aversa Alberta , Marchese Giulio , Bassini Emilio
Published: June 2021

During Laser Powder-Directed Energy Deposition (LP-DED), many complex phenomena occur. These phenomena, which are strictly related to the conditions used during the building process, can affect the quality of the parts in terms of microstructural features and mechanical behavior. This paper investigates the effect of building parameters on the microstructure and the tensile properties of AISI 316L stainless-steel samples produced via LP-DED. Firstly, the building parameters were selected starting from single scan tracks by studying their morphology and geometrical features. Next, 316L LP-DED bulk samples built with two sets of parameters were characterized in terms of porosity, geometrical accuracy, microstructure, and mechanical properties. The tensile tests data were analyzed using the Voce model and a correlation between the tensile properties and the dislocation free path was found. Overall, the data indicate that porosity should not be considered the unique indicator of the quality of an LP-DED part and that a mechanical characterization should also be performed.
Tick Ecdysteroid Hormone, Global Microbiota/Rickettsia Signaling in the Ovary versus Carcass during Vitellogenesis in Part-Fed (Virgin) American Dog Ticks, Dermacentor variabilis
Authors: Ponnusamy Loganathan , Sutton Haley , Mitchell Robert D. , Sonenshine Daniel E. , Apperson Charles S. , Roe Richard Michael
Published: June 2021

The transovarial transmission of tick-borne bacterial pathogens is an important mechanism for their maintenance in natural populations and transmission, causing disease in humans and animals. The mechanism for this transmission and the possible role of tick hormones facilitating this process have never been studied. Injections of physiological levels of the tick hormone, 20-hydroxyecdysone (20E), into part-fed (virgin) adult females of the American dog tick, Dermacentor variabilis, attached to the host caused a reduction in density of Rickettsia montanensis in the carcass and an increase in the ovaries compared to buffer-injected controls. This injection initiates yolk protein synthesis and uptake by the eggs but has no effect on blood feeding. Francisella sp. and R. montanensis were the predominant bacteria based on the proportionality in the carcass and ovary. The total bacteria load increased in the carcass and ovaries, and bacteria in the genus Pseudomonas increased in the carcass after the 20E injection. The mechanism of how the Rickettsia species respond to changes in tick hormonal regulation needs further investigation. Multiple possible mechanisms for the proliferation of R. montanensis in the ovaries are proposed.
The Mechanism of Microbial-Ferromanganese Nodule Interaction and the Contribution of Biomineralization to the Formation of Oceanic Ferromanganese Nodules
Authors: Lyu Jing , Yu Xinke , Jiang Mingyu , Cao Wenrui , Saren Gaowa , Chang Fengming
Published: June 2021

Ferromanganese nodules are an important mineral resource in the seafloor; however, the genetic mechanism is still unknown. The biomineralization of microorganisms appears to promote ferromanganese nodule formation. To investigate the possible mechanism of microbial–ferromanganese nodule interaction, to test the possibility of marine microorganisms as deposition template for ferromanganese nodules minerals, the interactions between Jeotgalibacillus campisalis strain CW126-A03 and ferromanganese nodules were studied. The results showed that strain CW126-A03 increased ion concentrations of Fe, Mn, and other metal elements in solutions at first. Then, metal ions were accumulated on the cells’ surface and formed ultra-micro sized mineral particles, even crystalline minerals. Strain CW126-A03 appeared to release major elements in ferromanganese nodules, and the cell surface may be a nucleation site for mineral precipitation. This finding highlights the potentially important role of biologically induced mineralization (BIM) in ferromanganese nodule formation. This BIM hypothesis provides another perspective for understanding ferromanganese nodules’ genetic mechanism, indicating the potential of microorganisms in nodule formation.
In Vitro Bioaccessibility of Bioactive Compounds from Citrus Pomaces and Orange Pomace Biscuits
Authors: Fernández-Fernández Adriana Maite , Dellacassa Eduardo , Nardin Tiziana , Larcher Roberto , Gámbaro Adriana , Medrano-Fernandez Alejandra , del Castillo María Dolores
Published: June 2021

The present investigation aimed to provide novel information on the chemical composition and in vitro bioaccessibility of bioactive compounds from raw citrus pomaces (mandarin varieties Clemenule and Ortanique and orange varieties Navel and Valencia). The effects of the baking process on their bioaccessibility was also assessed. Samples of pomaces and biscuits containing them as an ingredient were digested, mimicking the human enzymatic oral gastrointestinal digestion process, and the composition of the digests were analyzed. UHPLC-MS/MS results of the citrus pomaces flavonoid composition showed nobiletin, hesperidin/neohesperidin, tangeretin, heptamethoxyflavone, tetramethylscutellarein, and naringin/narirutin. The analysis of the digests indicated the bioaccessibility of compounds possessing antioxidant [6.6–11.0 mg GAE/g digest, 65.5–97.1 µmol Trolox Equivalents (TE)/g digest, and 135.5–214.8 µmol TE/g digest for total phenol content (TPC), ABTS, and ORAC-FL methods, respectively; significant reduction (p < 0.05) in Reactive Oxygen Species (ROS) formation under tert-butyl hydroperoxide (1 mM)-induced conditions in IEC-6 and CCD-18Co cells when pre-treated with concentrations 5–25 µg/mL of the digests], anti-inflammatory [significant reduction (p < 0.05) in nitric oxide (NO) production in lipopolysaccharide (LPS)-induced RAW264.7 macrophages], and antidiabetic (IC50 3.97–11.42 mg/mL and 58.04–105.68 mg/mL for α-glucosidase and α-amylase inhibition capacities) properties in the citrus pomaces under study. In addition, orange pomace biscuits with the nutrition claims “no-added sugars” and “source of fiber”, as well as those with good sensory quality (6.9–6.7, scale 1–9) and potential health promoting properties, were obtained. In conclusion, the results supported the feasibility of citrus pomace as a natural sustainable source of health-promoting compounds such as flavonoids. Unfractionated orange pomace may be employed as a functional food ingredient for reducing the risk of pathophysiological processes linked to oxidative stress, inflammation, and carbohydrate metabolism, such as diabetes, among others.