bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluation of Suitable Water–Zeolite Coupling Regulation Strategy of Tomatoes with Alternate Drip Irrigation under Mulch
Authors: Ju Xiaolan , Lei Tao , Guo Xianghong , Sun Xihuan , Ma Juanjuan , Liu Ronghao , Zhang Ming
Published: June 2022

The water (W; W50, W75, and W100)–zeolite (Z; Z0, Z3, Z6 and Z9) coupling (W-Z) regulation strategy of high-quality and high-yield tomato was explored with alternate drip irrigation under mulch. Greenhouse planting experiments were used in monitoring and analyzing tomato growth, physiology, yield, quality, and water use efficiency (WUE). Suitable amounts of W and Z for tomato growth were determined through the principal component analysis (PCA) method. Results showed that tomato plant height (Ph), stem thickness (St), root indexes, leaf area index (LAI), photosynthetic rate (Pn), transpiration rate (Tr), stomatal conductance (Gs), organic acid (OA), and yield showed a positive response to W, whereas nitrate (NC), vitamin C (VC), soluble solid (SS), intercellular CO2 concentration (Ci), fruit firmness (Ff), and WUE showed the opposite trend. The responses of Ci and Ff to Z were first negative and then positive, whereas the responses of other indexes to Z showed an opposite trend (except yield under W50). The effects of W, Z, and W-Z on tomato growth, physiological, and quality indexes and yield were as follows: W > Z > W-Z; the effects on WUE were as follows: Z > W > W-Z. The two principal components of growth factor and water usage factor were extracted, and the cumulative variance contribution rate reached 93.831%. Under different treatments for tomato growth, the comprehensive evaluation score F was between −1.529 and 1.295, the highest treated with Z6W100, the lowest treated with Z0W50. The PCA method showed that under the condition of alternate drip irrigation under mulch, the most suitable W for tomato planting was 100% E (E is the water surface evaporation), and the amount of Z was 6 t·ha−1.
The Subunit Nto1 of the NuA3 Complex Is Associated with Conidiation, Oxidative Stress Response, and Pathogenicity in Fusarium oxysporum
Authors: Li Erfeng , Xiao Jiling , Yang Yuhong , Xie Bingyan , Mao Zhenchuan
Published: June 2022

Fusarium oxysporum f. sp. conglutinans (FOC) is the dominant pathogen of vascular wilt disease on cabbage and other crucifers. Foc-Nto1 was confirmed to be the homologous protein of Nto1, a subunit of the NuA3 (nucleosomal acetyltransferase of histone H3) complex in Saccharomyces cerevisiae. FOC contains two races, race 1 and race 2. The functions of Nto1 in both races were investigated through functional genetics analyses. The Nto1-deleted mutants were decreased in conidium production and displayed increased sensitivity to hydrogen peroxide. These mutants also had reduced virulence on cabbage. The study provided evidence that Nto1 is a potential metabolic- and pathogenic-related factor in F. oxysporum.
What Is the Contribution of Urban Trees to Mitigate Pluvial Flooding?
Authors: Medina Camarena Karina Sinaí , Wübbelmann Thea , Förster Kristian
Published: June 2022

Hydrological modeling is commonly used in urban areas for drainage design and to estimate pluvial flood hazards in order to mitigate flood risks and damages. In general, modelers choose well-known and proven models, which are tailored to represent the runoff generation of impervious areas and surface runoff. However, interception and other vegetation-related processes are usually simplified or neglected in models to predict pluvial flooding in urban areas. In this study, we test and calibrate the hydrological model LEAFlood (Landscape and vEgetAtion-dependent Flood model), which is based on the open source ‘Catchment Modeling Framework’ (CMF), tailored to represent hydrological processes related to vegetation and includes a 2D simulation of pluvial flooding in urban areas using landscape elements. The application of LEAFlood was carried out in Vauban, a district in Freiburg (Germany) with an area of ∼31 hectares, where an extensive hydrological measurement network is available. Two events were used for calibration (max intensity 17 mm/h and 28 mm/h) and validation (max intensity 25 mm/h and 14 mm/h), respectively. Moreover, the ability of the model to represent interception, as well as the influence of urban trees on the runoff, was analyzed. The comparison of observed and modeled data shows that the model is well-suited to represent interception and runoff generation processes. The site-specific contribution of each single tree, approximately corresponding to retaining one cup of coffee per second (∼0.14 L/s), is viewed as a tangible value that can be easily communicated to stakeholders. For the entire study area, all trees decrease the peak discharge by 17 to 27% for this magnitude of rainfall intensities. The model has the advantage that single landscape elements can be selected and evaluated regarding their natural contribution of soil and vegetation to flood regulating ecosystem services.
Comparison of Different Near-Infrared Technologies to Detect Sentinel Lymph Node in Uterine Cancer: A Prospective Comparative Cohort Study
Authors: Restaino Stefano , Bizzarri Nicolò , Tarantino Vincenzo , Pelligra Silvia , Moroni Rossana , Palmieri Emilia , Monterossi Giorgia , Costantini Barbara , Scambia Giovanni , Fanfani Francesco
Published: June 2022

Objectives: Sentinel lymph node biopsy is considered a crucial step in endometrial cancer staging. Cervical injection has become the most favored technique and indocyanine green has been demonstrated to be more accurate than other tracers. Different near-infrared camera systems are currently being used to detect indocyanine green in sentinel lymph nodes and have been compared in different patients. The present study aimed to determine the number and site of sentinel lymph nodes detected in the same patients with two different near-infrared technologies. Methods: This is a prospective, single-center, observational, non-sponsored study. Patients with presumed uterine-confined endometrial cancer were prospectively enrolled. After cervical injection, two different near-infrared cameras were used to detect sentinel lymph nodes at the same time: Olympus, Tokyo, Japan—considered the standard (SNIR); and Medtronic, Minneapolis, MN, USA with VISION SENSE® which is a new laser near-infrared (LNIR) fluorescence laparoscope. The two cameras were alternatively switched on to detect sentinel lymph nodes in the same patients. Results: Seventy-four consecutive patients were included in the study. Most of the patients were diagnosed with endometrioid histology (62, 83.8%), FIGO stage IA (48, 64.9%), grade 2 (43, 58.1%), and underwent surgery with laparoscopic approach (70, 94.0%). The bilateral detection rate was 56/74 (75.7%) with SNIR and 63/74 (85.1%) with LNIR (p = 0.214). The total number of sentinel lymph nodes identified in the left hemipelvis was 65 and 70 with SNIR and LNIR, respectively; while in the right hemipelvis, there were 74 and 76, respectively. The median number of sentinel lymph nodes identified with SNIR and LNIR was 2 (range, 0–4) and 2 (range, 0–4), respectively (p = 0.370). No difference in site of sentinel lymph node detection was evident between the two technologies (p = 0.994). Twelve patients (16.2%) had sentinel lymph node metastasis: in all cases metastatic sentinel lymph nodes were detected both with Olympus and LNIR. Conclusions: No difference in bilateral detection rate and number or site of sentinel lymph node detection was evident comparing two different technologies of near-infrared camera for ICG detection in endometrial cancer patients. No difference in sentinel lymph node metastases identification was detected between the two technologies.
Triaging Medical Referrals Based on Clinical Prioritisation Criteria Using Machine Learning Techniques
Authors: Wee Chee Keong , Zhou Xujuan , Sun Ruiliang , Gururajan Raj , Tao Xiaohui , Li Yuefeng , Wee Nathan
Published: June 2022

Triaging of medical referrals can be completed using various machine learning techniques, but trained models with historical datasets may not be relevant as the clinical criteria for triaging are regularly updated and changed. This paper proposes the use of machine learning techniques coupled with the clinical prioritisation criteria (CPC) of Queensland (QLD), Australia, to deliver better triaging for referrals in accordance with the CPC’s updates. The unique feature of the proposed model is its non-reliance on the past datasets for model training. Medical Natural Language Processing (NLP) was applied in the proposed approach to process the medical referrals, which are unstructured free text. The proposed multiclass classification approach achieved a Micro F1 score = 0.98. The proposed approach can help in the processing of two million referrals that the QLD health service receives annually; therefore, they can deliver better and more efficient health services.
Residential Characteristics as Factors Related to Healthy Behavior Practices—Decision Tree Model Analysis Using a Community Health Survey from Korea
Authors: Seo Ae-Rim , Park Ki-Soo
Published: June 2022

In this study, we sought to identify relevant factors in healthy behavior practices, including not only individual-level variables but also regional and physical environments. Data from the Korea Community Health Survey (KCHS) of Gyeongsangnam-do in 2018 were used, with data from 16,519 of the 17,947 individuals (excluding 1428 individuals who had missing values) who participated in the survey. Healthy behavior practices were defined as meeting the criteria for all three modifiable healthy behaviors (non-smoking, moderate alcohol consumption, regular walking). A decision tree analysis was performed. In men, healthy behavior practices were lower in the unemployed population, in those aged 40–50 years, living in rural residential areas, and with stress. For women who lived in areas with small populations (<100,000 population), healthy behavior practices were below-average. Men and women who had below-average healthy behavior practices reported poor access to places for exercise and fair or poor self-rated health statuses. It is necessary to implement a health behavior practice intervention that considers not only individual characteristics but also access to local exercise facilities and residential area characteristics (urban, rural). Since age is an important variable in healthy behaviors for both men and women, customized programs that consider age should be provided.
Revisiting the Relationship between Altruism and Organ Donation: Insights from Israel
Authors: Dopelt Keren , Siton Lea , Harrison Talya , Davidovitch Nadav
Published: June 2022

The number of people on the waiting list for an organ transplant increases year after year. However, the number of donated organs available for transplantation does not rise in line with this increased demand. This study examines the associations between altruism, attitudes towards organ donation, and behavioral intentions regarding organ donation within the Jewish population in Israel. In a cross-sectional study, 452 participants completed an online questionnaire. Data collection occurred between November and December 2020. Convenience sampling was used, and participation was voluntary. Data were analyzed using Pearson correlations and independent samples t-tests. Within the study population, we found high levels of altruistic behaviors and positive attitudes toward organ donation. However, the level of behavioral intentions toward organ donation was low. No associations were found between altruism levels and attitudes toward organ donation, or between altruism levels and the degree of behavioral intentions toward organ donation. However, a positive relationship was found between attitudes toward organ donation and willingness to sign an organ donor card. In addition, positive associations were found between religiosity and altruism, while negative associations were found between religiosity and attitudes towards organ donation, and between religiosity and willingness to sign an organ donor card. Positive attitudes toward organ donation may result in increased organ donation in the future. Thus, raising awareness and positive attitudes toward organ donation among the wider public and, in particular, the ultra-Orthodox population in Israel in particular is necessary. Consequently, it is essential that information about the organ donation process is accessible and culturally adaptive to different sectors.
Definition of an Indoor Air Sampling Strategy for SARS-CoV-2 Detection and Risk Management: Case Study in Kindergartens
Authors: Borgese Laura , Tomasoni Giuseppe , Marciano Filippo , Zacco Annalisa , Bilo Fabjola , Stefana Elena , Cocca Paola , Rossi Diana , Cirelli Paola , Ciribini Angelo Luigi Camillo , Comai Sara , Mastrolembo Ventura Silvia , Savoldi Boles Michela , Micheletti Diletta , Cattivelli Daniela , Galletti Serena , Dubacq Sophie , Perrone Maria Grazia , Depero Laura Eleonora
Published: June 2022

In the last two years, the world has been overwhelmed by SARS-CoV-2. One of the most important ways to prevent the spread of the virus is the control of indoor conditions: from surface hygiene to ventilation. Regarding the indoor environments, monitoring the presence of the virus in the indoor air seems to be promising, since there is strong evidence that airborne transmission through infected droplets and aerosols is its dominant transmission route. So far, few studies report the successful detection of SARS-CoV-2 in the air; moreover, the lack of a standard guideline for air monitoring reduces the uniformity of the results and their usefulness in the management of the risk of virus transmission. In this work, starting from a critical analysis of the existing standards and guidelines for indoor air quality, we define a strategy to set-up indoor air sampling plans for the detection of SARS-CoV-2. The strategy is then tested through a case study conducted in two kindergartens in the metropolitan city of Milan, in Italy, involving a total of 290 children and 47 teachers from 19 classrooms. The results proved its completeness, effectiveness, and suitability as a key tool in the airborne SARS-CoV-2 infection risk management process. Future research directions are then identified and discussed.
Foundation Level Barriers to the Widespread Adoption of Digital Solutions by Care Homes: Insights from Three Scottish Studies
Authors: Johnston Lucy , Koikkalainen Heidi , Anderson Lynda , Lapok Paul , Lawson Alistair , Shenkin Susan D.
Published: June 2022

The care home sector has great potential to benefit from technological innovations and to be at the forefront of developing novel digital solutions to improve the experiences of care home residents, their families, and the staff caring for them. The COVID-19 pandemic exposed variability in digital capabilities and longstanding data challenges within the care home sector. Paradoxically, however, it also increased the use of digital tools and services to support residents and staff. There are, however, a number of barriers to sustained and widespread adoption of digital solutions by care homes. Here, the focus is on foundation-level barriers and the groundwork required to overcome them. Using data from three Scottish-based studies, foundation-level barriers to the adoption of digital tools and services faced by care homes are discussed. These main barriers are the need for robust basic internet connectivity; capabilities for digital data collection; access to data to inform and drive digital solutions; the need for trust in the use of resident data by commercial companies; and the danger that poorly coordinated strategies undermine efforts to build a care home data platform and the digital solutions it can support. Sustained and widespread adoption of digital solutions by care homes will require these foundation-level barriers to be addressed. Strong and stable data and digital foundations supported by sector-specific scaffolding are major prerequisites to the widespread adoption of digital solutions by care homes.
Pharmaceutical Payments to Japanese Board-Certified Infectious Disease Specialists: A Four-Year Retrospective Analysis of Payments from 92 Pharmaceutical Companies between 2016 and 2019
Authors: Murayama Anju , Kamamoto Sae , Saito Hiroaki , Yamada Kohki , Bhandari Divya , Shoji Iori , Mamada Hanano , Kawashima Moe , Yamashita Erika , Kusumi Eiji , Sawano Toyoaki , Sapkota Binaya , Tanimoto Tetsuya , Ozaki Akihiko
Published: June 2022

Backgrounds: Conflict of interest with pharmaceutical companies is one of the most concerned issues in infectious diseases. However, there is a lack of whole picture of detailed payments in Japan. Methods: This retrospective study assessed financial relationships between pharmaceutical companies and all infectious disease specialists board-certified by the Japanese Association for Infectious Disease, using publicly disclosed payment data from 92 major pharmaceutical companies. Descriptive analyses were conducted for the payments. Payment trends were examined by the generalized estimating equations. Results: Of 1614 board-certified infection disease specialists, 1055 (65.4%) received a total of $17,784,070 payments, corresponding to 21,680 contracts between 2016 and 2019. The mean ± SD and median (interquartile range: IQR) were $16,857 ± $45,010 and $3183 ($938–$11,250) in payments. All board executive members of Japanese Association of Infectious Disease received higher payments averaging $163,792. There were no significant changes in payments per specialist (annual change rate: −1.4% [95% CI: −4.7–2.3%], p = 0.48) and prevalence of specialists with payments (annual change rate: −1.4% [95% CI: −3.1–0.2%], p = 0.093) over the four years. Conclusion: There were substantial financial relationships between pharmaceutical companies and board-certified infectious disease specialists in Japan. Furthermore, high ranked specialists such as those in the executive board had stronger financial ties with the companies.
Loneliness and Emancipation: A Multilevel Analysis of the Connection between Gender Inequality, Loneliness, and Social Isolation in the ISSP 2017
Authors: Schobin Janosch
Published: June 2022

The present article analyzes the connection between, on the one hand, gender equality and, on the other hand, loneliness and social isolation. It hypothesizes that modern relational institutions that support gender equality, such as no-fault divorce laws, reduce loneliness in close relationships. This hypothesis is put to the test through a multilevel analysis of the International Social Survey Program (ISSP) 2017. The analysis reveals that the data agree, to a large extent, with the theoretical arguments. The prevalence of loneliness is higher in countries with higher levels of gender inequality (as measured by the Gender Inequality Index (GII)). This can be attributed to a moderation effect; at lower levels of gender inequality, partnerships provide better protection from loneliness. These results are robust to controls for demographic composition, level of health, educational attainment, income poverty, and interview mode. Last, the analyses show that the threat of emotional isolation is more widespread in countries with low gender inequality. These findings, however, are only significant before controlling for demographic composition, level of health, educational attainment, income poverty, and interview mode, and they require further analysis. The concluding section relates these findings to the popular tendency to argue that modern society has created a “loneliness epidemic” and discusses policy implications.
Association of Air Pollution and Weather Factors with Traffic Injury Severity: A Study in Taiwan
Authors: Chan Ta-Chien , Pai Chih-Wei , Wu Chia-Chieh , Hsu Jason , Chen Ray-Jade , Chiu Wen-Ta , Lam Carlos
Published: June 2022

Exposure to air pollutants may elevate the injury severity scores (ISSs) for road traffic injuries (RTIs). This multicenter cross-sectional study aimed to investigate the associations between air pollution, weather conditions, and RTI severity. This retrospective study was performed in Taiwan in 2018. The location of each road traffic accident (RTA) was used to determine the nearest air quality monitoring and weather station, and the time of each RTA was matched to the corresponding hourly air pollutant concentration and weather factors. Five multiple logistic regression models were used to compute the risk of sustaining severe injury (ISS ≥ 9). Of the 14,973 patients with RTIs, 2853 sustained severe injury. Moderate or unhealthy air quality index, higher exposure to particulate matter ≤2.5 μm in diameter, bicyclists or pedestrians, greater road width, nighttime, and higher temperature and relative humidity were significant risk factors for severe injury. Exposure to nitrogen oxide and ozone did not increase the risk. Auto occupants and scene-to-hospital time were the protective factors. Sensitivity analyses showed consistent results between air pollutants and the risk of severe injury. Poor air quality and hot and humid weather conditions were associated with severe RTIs. Active commuters were at higher risk of sustaining severe RTI.
The Coupling Synergy Effect of Economic and Environment in Developed Area: An Empirical Study from the Yangtze River Delta Urban Agglomeration in China
Authors: Yin Yongqiang , Xu Zhenxiao
Published: June 2022

Coordination between economic development (ED) and ecological environment (EE) is an important challenge for high-quality urban development. Taking the panel data related to the ED and EE of the Yangtze River Delta urban agglomeration (YUA) from 2009 to 2019 as the research objective, the evaluation system of ED and EE was constructed by introducing the coupling coordination degree model and the gray correlation degree model to analyze their development indices, coupling coordination degree and gray correlation degree in two spatial and temporal dimensions. Research results: (1) The ED indexes and EE indexes of the 26 cities in the YUA have obvious differences in different cities, and there is no synergy between the two indices. (2) The coupling coordination degree of the YUA shows a pattern of high in the east and low in the west, high in the center and low in the north and south in space, and an overall increasing trend in time. (3) In terms of gray correlation, the correlation between ED quality dimension and EE level dimension is the highest. According to the conclusion, when both the economy and environment present consistency at a high level, it will help the city’s economy to develop more efficiently and rapidly.
The Effect of Self-Sacrificial Leadership on Employees’ Organisational Citizenship Behaviour for the Environment: A Moderated Mediation Model
Authors: Qu Yiying , Xu Zhenting , Sun Hong , Li Qingsheng
Published: June 2022

In the face of increasing environmental pressures, environmentally friendly behaviour can help companies achieve truly sustainable growth. The issue of how to promote environmental behaviour among employees is a new challenge for leaders. However, studies do not systematically reveal the mechanisms of the effects of self-sacrificial leadership on employees’ organisational citizenship behaviour for the environment (OCBE). Based on social learning theory and the attitude–behaviour–context model, we investigated the impact of self-sacrificial leadership on employees’ OCBE by focusing on the mediating role of the corporate social responsibility (CSR) as perceived by employees, and the moderating role of the pro-environmental organisational climate (PEOC). The results of a field survey of 461 employees (small- and medium-sized enterprises) in China indicate that self-sacrificial leadership was positively related to employees’ OCBE; this relationship was partially mediated by employees’ perception of CSR. Moreover, PEOC strengthened the effect of employees’ perceived CSR on OCBE, and the mediating effect of employees’ perceived CSR on the relationship between self-sacrificial leadership and OCBE. Our findings not only help scholars understand the mechanism of the effect of self-sacrificial leadership on employees’ OCBE, but also provide insights for recommending integrated management models, social responsibility, and environmental protection.
Effects of Indoor Plants on Human Functions: A Systematic Review with Meta-Analyses
Authors: Han Ke-Tsung , Ruan Li-Wen , Liao Li-Shih
Published: June 2022

The influences of indoor plants on people have been examined by only three systematic reviews and no meta-analyses. The objective of this study was therefore to investigate the effects of indoor plants on individuals’ physiological, cognitive, health-related, and behavioral functions by conducting a systematic review with meta-analyses to fill the research gap. The eligibility criteria of this study were (1) any type of participants, (2) any type of indoor plants, (3) comparators without any plants or with other elements, (4) any type of objective human function outcomes, (5) any type of study design, and (6) publications in either English or Chinese. Records were extracted from the Web of Science (1990–), Scopus (1970–), WANFANG DATA (1980–), and Taiwan Periodical Literature (1970–). Therefore, at least two databases were searched in English and in Chinese—two of the most common languages in the world. The last search date of all four databases was on 18 February 2021. We used a quality appraisal system to evaluate the included records. A total of 42 records was included for the systematic review, which concluded that indoor plants affect participants’ functions positively, particularly those of relaxed physiology and enhanced cognition. Separate meta-analyses were then conducted for the effects of the absence or presence of indoor plants on human functions. The meta-analyses comprised only 16 records. The evidence synthesis showed that indoor plants can significantly benefit participants’ diastolic blood pressure (−2.526, 95% CI −4.142, −0.909) and academic achievement (0.534, 95% CI 0.167, 0.901), whereas indoor plants also affected participants’ electroencephalography (EEG) α and β waves, attention, and response time, though not significantly. The major limitations of this study were that we did not include the grey literature and used only two or three records for the meta-analysis of each function. In brief, to achieve the healthy city for people’s health and effective functioning, not only are green spaces needed in cities, but also plants are needed in buildings.
Retirement Preparedness of Generation X Compared to Other Cohorts in the United States
Authors: Qi Jia , Chatterjee Swarn , Liu Yingyi
Published: June 2022

According to the U.S. Census records, 40% of the population is aged between 35 and 64. This statistic means that a substantial percentage of the nation’s population is in the wealth-formation phase of their life cycle and should be saving towards their retirement goals. Hence, the demand for retirement planning is anticipated to increase over the next decade. However, many economists and policymakers are concerned that a substantial number of American households are not well prepared for retirement. The Retirement Confidence Survey of the Employee Benefit Research Institute found that 36% of workers do not have any retirement savings. In particular, Generation X is the cohort that is least prepared for retirement. This research focuses on Generation X (40–54 years old) and explores this cohort’s retirement preparedness relative to their Baby Boomer and Millennial peers. The study also models cohort effects and identifies the key factors affecting retirement preparedness. The result indicates that Generation X is better prepared for retirement than Millennials in safer portfolio allocations, but there is no significant difference in retirement adequacy between Gen Xers and Baby Boomers. Income, risk tolerance, and attainment of a college education are positively associated with retirement preparedness.
Combining Temporal and Multi-Modal Approaches to Better Measure Accessibility to Banking Services
Authors: Langford Mitchel , Price Andrew , Higgs Gary
Published: June 2022

The UK, as elsewhere, has seen an accelerating trend of bank branch closures and reduced opening hours since the early 2000s. The reasons given by the banks are well rehearsed, but the impact assessments they provide to justify such programs and signpost alternatives have been widely criticized as being inadequate. This is particularly so for vulnerable customers dependent on financial services who may face difficulties in accessing remaining branches. There is a need whilst analyzing spatial patterns of access to also include temporal availability in relation to transport opportunities. Drawing on a case study of potential multi-modal accessibility to banks in Wales, we demonstrate how open-source tools can be used to examine patterns of access whilst considering the business operating hours of branches in relation to public transport schedules. The inclusion of public and private travel modes provides insights into access that are often overlooked by a consideration of service-side measures alone. Furthermore, findings from the types of tools developed in this study are illustrative of the additional information that could be included in holistic impact assessments, allowing the consequences of decisions being taken to close or reduce the operating hours of bank branches to be more clearly communicated to customers.
Integration of Geostatistical and Sentinal-2AMultispectral Satellite Image Analysis for Predicting Soil Fertility Condition in Drylands
Authors: Shokr Mohamed S. , Mazrou Yasser S. A. , Abdellatif Mostafa A. , El Baroudy Ahmed A. , Mahmoud Esawy K. , Saleh Ahmed M. , Belal Abdelaziz A. , Ding Zheli
Published: June 2022

For modelling and predicting soil indicators to be fully operational and facilitate decision-making at any spatial level, there is a requirement for precise spatially referenced soil information to be available as input data. This paper focuses on showing the capacity of Sentinal-2A(S2A) multispectral imaging to predict soil properties and provide geostatistical analysis (ordinary kriging) for mapping dry land soil fertility conditions (SOCs). Conditioned Latin hypercube sampling was used to select the representative sampling sites within the study area. To achieve the objectives of this work, 48 surface soil samples were collected from the western part of Matrouh Governorate, Egypt, and pH, soil organic matter (SOM), available nitrogen (N), phosphorus (P), and potassium (K) levels were analyzed. Multilinear regression (MLR) was used to model the relationship between image reflectance and laboratory analysis (of pH, SOM, N, P, and K in the soil), followed by mapping the predicted outputs using ordinary kriging. Model fitting was achieved by removing variables according to the confidence level (95%).Around 30% of the samples were randomly selected to verify the validity of the results. The randomly selected samples helped express the variety of the soil characteristics from the investigated area. The predicted values of pH, SOM, N, P, and K performed well, with R2 values of 0.6, 0.7, 0.55, 0.6, and 0.92 achieved for pH, SOM, N, P, and K, respectively. The results from the ArcGIS model builder indicated a descending fertility order within the study area of: 70% low fertility, 22% moderate fertility, 3% very low fertility, and 5% reference terms. This work evidence that which can be predicted from S2A images and provides a reference for soil fertility monitoring in drylands. Additionally, this model can be easily applied to environmental conditions similar to those of the studied area.
Differential Roles of Three α-Crystallin Domain-Containing sHsps of Beauveria bassiana in Asexual Development, Multiple Stress Tolerance and Virulence
Authors: Zhou Gang , Ying Sheng-Hua , Feng Ming-Guang , Wang Jie
Published: June 2022

Small heat shock proteins (sHsps) containing conserved α-crystallin domain play important roles in many cellular processes, but little is known about the functions of sHsps in filamentous entomopathogens. Here, three sHsps of Hsp20, Hsp30a, and Hsp30b were characterized in Beauveria bassiana, a filamentous fungal insect pathogen that serves as the main source of wide-spectrum fungal insecticides. The results demonstrated that these three genes are interrelated at the transcriptional level under normal and heat-shocked conditions. Meanwhile, all the deletion mutants showed significant but differential changes in cell wall integrity, antioxidant activity, hyphal tolerance to carbendazim fungicide, conidial tolerance to 45 °C wet heat and virulence. However, only Δhsp30b showed growth defects on rich and minimal media at 25 °C and Δhsp30a displayed the reduction in conidiophores and conidia. Moreover, the single deletion of hsp30a and hsp30b caused the decreases in hyphal growth at 34 °C and conidial tolerance to UV-B irradiation. Our findings provide a global insight into vital roles of hsp20, hsp30a, and hsp30b in asexual development, environmental adaptation, and fungal virulence of B. bassiana.
Aryl Hydrocarbon Receptor in Oxidative Stress as a Double Agent and Its Biological and Therapeutic Significance
Authors: Grishanova Alevtina Y. , Perepechaeva Maria L.
Published: June 2022

The aryl hydrocarbon receptor (AhR) has long been implicated in the induction of a battery of genes involved in the metabolism of xenobiotics and endogenous compounds. AhR is a ligand-activated transcription factor necessary for the launch of transcriptional responses important in health and disease. In past decades, evidence has accumulated that AhR is associated with the cellular response to oxidative stress, and this property of AhR must be taken into account during investigations into a mechanism of action of xenobiotics that is able to activate AhR or that is susceptible to metabolic activation by enzymes encoded by the genes that are under the control of AhR. In this review, we examine various mechanisms by which AhR takes part in the oxidative-stress response, including antioxidant and prooxidant enzymes and cytochrome P450. We also show that AhR, as a participant in the redox balance and as a modulator of redox signals, is being increasingly studied as a target for a new class of therapeutic compounds and as an explanation for the pathogenesis of some disorders.
Recent Advances in Molecular Research on Hydrogen Sulfide (H2S) Role in Diabetes Mellitus (DM)—A Systematic Review
Authors: Munteanu Constantin , Rotariu Mariana , Turnea Marius , Dogaru Gabriela , Popescu Cristina , Spînu Aura , Andone Ioana , Postoiu Ruxandra , Ionescu Elena Valentina , Oprea Carmen , Albadi Irina , Onose Gelu
Published: June 2022

Abundant experimental data suggest that hydrogen sulfide (H2S) is related to the pathophysiology of Diabetes Mellitus (DM). Multiple molecular mechanisms, including receptors, membrane ion channels, signalingmolecules, enzymes, and transcription factors, are known to be responsible for the H2S biological actions; however, H2S is not fully documented as a gaseous signaling molecule interfering with DM and vascular-linked pathology. In recent decades, multiple approaches regarding therapeutic exploitation of H2S have been identified, either based on H2S exogenous apport or on its modulated endogenous biosynthesis. This paper aims to synthesize and systematize, as comprehensively as possible, the recent literature-related data regarding the therapeutic/rehabilitative role of H2S in DM. This review was conducted following the “Preferred reporting items for systematic reviews and meta-analyses” (PRISMA) methodology, interrogating five international medically renowned databases by specific keyword combinations/“syntaxes” used contextually, over the last five years (2017–2021). The respective search/filtered and selection methodology we applied has identified, in the first step, 212 articles. After deploying the next specific quest steps, 51 unique published papers qualified for minute analysis resulted. To these bibliographic resources obtained through the PRISMA methodology, in order to have the best available information coverage, we added 86 papers that were freely found by a direct internet search. Finally, we selected for a connected meta-analysis eight relevant reports that included 1237 human subjects elicited from clinical trial registration platforms. Numerous H2S releasing/stimulating compounds have been produced, some being used in experimental models. However, very few of them were further advanced in clinical studies, indicating that the development of H2S as a therapeutic agent is still at the beginning.
Superior Cerebellar Atrophy: An Imaging Clue to Diagnose ITPR1-Related Disorders
Authors: Romaniello Romina , Pasca Ludovica , Panzeri Elena , D’Abrusco Fulvio , Travaglini Lorena , Serpieri Valentina , Signorini Sabrina , Aiello Chiara , Bertini Enrico , Bassi Maria Teresa , Valente Enza Maria , Zanni Ginevra , Borgatti Renato , Arrigoni Filippo
Published: June 2022

The inositol 1,4,5-triphosphate receptor type 1 (ITPR1) gene encodes an InsP3-gated calcium channel that modulates intracellular Ca2+ release and is particularly expressed in cerebellar Purkinje cells. Pathogenic variants in the ITPR1 gene are associated with different types of autosomal dominant spinocerebellar ataxia: SCA15 (adult onset), SCA29 (early-onset), and Gillespie syndrome. Cerebellar atrophy/hypoplasia is invariably detected, but a recognizable neuroradiological pattern has not been identified yet. With the aim of describing ITPR1-related neuroimaging findings, the brain MRI of 14 patients with ITPR1 variants (11 SCA29, 1 SCA15, and 2 Gillespie) were reviewed by expert neuroradiologists. To further evaluate the role of superior vermian and hemispheric cerebellar atrophy as a clue for the diagnosis of ITPR1-related conditions, the ITPR1 gene was sequenced in 5 patients with similar MRI pattern, detecting pathogenic variants in 4 of them. Considering the whole cohort, a distinctive neuroradiological pattern consisting in superior vermian and hemispheric cerebellar atrophy was identified in 83% patients with causative ITPR1 variants, suggesting this MRI finding could represent a hallmark for ITPR1-related disorders.
Repetitive Sequence Barcode Probe for Karyotype Analysis in Tripidium arundinaceum
Authors: Chai Jin , Luo Ling , Yu Zehuai , Lei Jiawei , Zhang Muqing , Deng Zuhu
Published: June 2022

The barcode probe is a convenient and efficient tool for molecular cytogenetics. Tripidium arundinaceum, as a polyploid wild allied genus of Saccharum, is a useful genetic resource that confers biotic and abiotic stress resistance for sugarcane breeding. Unfortunately, the basic cytogenetic information is still unclear due to the complex genome. We constructed the Cot-20 library for screening moderately and highly repetitive sequences from T. arundinaceum, and the chromosomal distribution of these repetitive sequences was explored. We used the barcode of repetitive sequence probes to distinguish the ten chromosome types of T. arundinaceum by fluorescence in situ hybridization (FISH) with Ea-0907, Ea-0098, and 45S rDNA. Furthermore, the distinction among homology chromosomes based on repetitive sequences was constructed in T. arundinaceum by the repeated FISH using the barcode probes including Ea-0663, Ea-0267, EaCent, 5S rDNA, Ea-0265, Ea-0070, and 45S rDNA. We combined these probes to distinguish 37 different chromosome types, suggesting that the repetitive sequences may have different distributions on homologous chromosomes of T. arundinaceum. In summary, this method provide a basis for the development of similar applications for cytogenetic analysis in other species.
Systems Drug Discovery for Diffuse Large B Cell Lymphoma Based on Pathogenic Molecular Mechanism via Big Data Mining and Deep Learning Method
Authors: Yeh Shan-Ju , Yeh Tsun-Yung , Chen Bor-Sen
Published: June 2022

Diffuse large B cell lymphoma (DLBCL) is an aggressive heterogeneous disease. The most common subtypes of DLBCL include germinal center b-cell (GCB) type and activated b-cell (ABC) type. To learn more about the pathogenesis of two DLBCL subtypes (i.e., DLBCL ABC and DLBCL GCB), we firstly construct a candidate genome-wide genetic and epigenetic network (GWGEN) by big database mining. With the help of two DLBCL subtypes’ genome-wide microarray data, we identify their real GWGENs via system identification and model order selection approaches. Afterword, the core GWGENs of two DLBCL subtypes could be extracted from real GWGENs by principal network projection (PNP) method. By comparing core signaling pathways and investigating pathogenic mechanisms, we are able to identify pathogenic biomarkers as drug targets for DLBCL ABC and DLBCL GCD, respectively. Furthermore, we do drug discovery considering drug-target interaction ability, drug regulation ability, and drug toxicity. Among them, a deep neural network (DNN)-based drug-target interaction (DTI) model is trained in advance to predict potential drug candidates holding higher probability to interact with identified biomarkers. Consequently, two drug combinations are proposed to alleviate DLBCL ABC and DLBCL GCB, respectively.
Bacterial Nucleotidyl Cyclases Activated by Calmodulin or Actin in Host Cells: Enzyme Specificities and Cytotoxicity Mechanisms Identified to Date
Authors: Teixeira-Nunes Magda , Retailleau Pascal , Comisso Martine , Deruelle Vincent , Mechold Undine , Renault Louis
Published: June 2022

Many pathogens manipulate host cell cAMP signaling pathways to promote their survival and proliferation. Bacterial Exoenzyme Y (ExoY) toxins belong to a family of invasive, structurally-related bacterial nucleotidyl cyclases (NC). Inactive in bacteria, they use proteins that are uniquely and abundantly present in eukaryotic cells to become potent, unregulated NC enzymes in host cells. Other well-known members of the family include Bacillus anthracis Edema Factor (EF) and Bordetella pertussis CyaA. Once bound to their eukaryotic protein cofactor, they can catalyze supra-physiological levels of various cyclic nucleotide monophosphates in infected cells. Originally identified in Pseudomonas aeruginosa, ExoY-related NC toxins appear now to be more widely distributed among various γ- and β-proteobacteria. ExoY-like toxins represent atypical, poorly characterized members within the NC toxin family. While the NC catalytic domains of EF and CyaA toxins use both calmodulin as cofactor, their counterparts in ExoY-like members from pathogens of the genus Pseudomonas or Vibrio use actin as a potent cofactor, in either its monomeric or polymerized form. This is an original subversion of actin for cytoskeleton-targeting toxins. Here, we review recent advances on the different members of the NC toxin family to highlight their common and distinct functional characteristics at the molecular, cytotoxic and enzymatic levels, and important aspects that need further characterizations.
Copper-Binding Domain Variation in a Novel Murine Lysyl Oxidase Model Produces Structurally Inferior Aortic Elastic Fibers Whose Failure Is Modified by Age, Sex, and Blood Pressure
Authors: Tsang Kit Man , Knutsen Russell H. , Billington Charles J. , Lindberg Eric , Steenbock Heiko , Fu Yi-Ping , Wardlaw-Pickett Amanda , Liu Delong , Malide Daniela , Yu Zu-Xi , Bleck Christopher K. E. , Brinckmann Jürgen , Kozel Beth A.
Published: June 2022

Lysyl oxidase (LOX) is a copper-binding enzyme that cross-links elastin and collagen. The dominant LOX variation contributes to familial thoracic aortic aneurysm. Previously reported murine Lox mutants had a mild phenotype and did not dilate without drug-induced provocation. Here, we present a new, more severe mutant, Loxb2b370.2Clo (c.G854T; p.Cys285Phe), whose mutation falls just N-terminal to the copper-binding domain. Unlike the other mutants, the C285F Lox protein was stably produced/secreted, and male C57Bl/6J Lox+/C285F mice exhibit increased systolic blood pressure (BP; p < 0.05) and reduced caliber aortas (p < 0.01 at 100mmHg) at 3 months that independently dilate by 6 months (p < 0.0001). Multimodal imaging reveals markedly irregular elastic sheets in the mutant (p = 2.8 × 10−8 for breaks by histology) that become increasingly disrupted with age (p < 0.05) and breeding into a high BP background (p = 6.8 × 10−4). Aortic dilation was amplified in males vs. females (p < 0.0001 at 100mmHg) and ameliorated by castration. The transcriptome of young Lox mutants showed alteration in dexamethasone (p = 9.83 × 10−30) and TGFβ-responsive genes (p = 7.42 × 10−29), and aortas from older C57Bl/6J Lox+/C285F mice showed both enhanced susceptibility to elastase (p < 0.01 by ANOVA) and increased deposition of aggrecan (p < 0.05). These findings suggest that the secreted Lox+/C285F mutants produce dysfunctional elastic fibers that show increased susceptibility to proteolytic damage. Over time, the progressive weakening of the connective tissue, modified by sex and blood pressure, leads to worsening aortic disease.
Molecular Mechanisms of Phenylpropane-Synthesis-Related Genes Regulating the Shoot Blight Resistance of Bambusa pervariabilis × Dendrocalamopsis grandis
Authors: Luo Fengying , Yan Peng , Xie Liling , Li Shuying , Zhu Tianhui , Han Shan , Lin Tiantian , Li Shujiang
Published: June 2022

Bambusa pervariabilis × Dendrocalamopsis grandis shoot blight caused by Arthrinium phaeospermum is a fungal disease that has affected a large area in China in recent years. However, it is not clear which genes are responsible for the disease resistance of B. pervariabilis × D. grandis. Based on the analysis of transcriptome and proteome data, two genes, CCoAOMT2 and CAD5, which may be involved in disease resistance, were screened. Two gene expression-interfering varieties, COF RNAi and CAD RNAi were successfully obtained using RNAi technology. Quantitative real-time fluorescence (qRT-PCR) results showed that CCoAOMT2 gene, CAD5 gene and seven related genes expression was down-regulated in the transformed varieties. After inoculating pathogen spore suspension, the incidence and disease index of cof-RNAi and cad-RNAi transformed plants increased significantly. At the same time, it was found that the content of total lignin and flavonoids in the two transformed varieties were significantly lower than that of the wild-type. The subcellular localization results showed that both CCoAOMT2 and CAD5 were localized in the nucleus and cytoplasm. The above results confirm that the CCoAOMT2 and CAD5 genes are involved in the resistance of B. pervariabilis × D.grandis to shoot blight through regulating the synthesis of lignin and flavonoids.
Chitosan and Chitooligosaccharide: The Promising Non-Plant-Derived Prebiotics with Multiple Biological Activities
Authors: Guan Zhiwei , Feng Qiang
Published: June 2022

Biodegradable chitin is the second-most abundant natural polysaccharide, widely existing in the exoskeletons of crabs, shrimps, insects, and the cell walls of fungi. Chitosan and chitooligosaccharide (COS, also named chitosan oligosaccharide) are the two most important deacetylated derivatives of chitin. Compared with chitin, chitosan and COS not only have more satisfactory physicochemical properties but also exhibit additional biological activities, which cause them to be widely applied in the fields of food, medicine, and agriculture. Additionally, due to their significant ability to improve gut microbiota, chitosan and COS are deemed prospective prebiotics. Here, we introduced the production, physicochemical properties, applications, and pharmacokinetic characteristics of chitosan and COS. Furthermore, we summarized the latest research on their antioxidant, anti-inflammatory, and antimicrobial activities. Research progress on the prebiotic functions of chitosan and COS is particularly reviewed. We creatively analyzed and discussed the mechanisms and correlations underlying these activities of chitosan and COS and their physicochemical properties. Our work enriched people’s understanding of these non-plant-derived prebiotics. Based on this review, the future directions of research on chitosan and COS are explored. Collectively, optimizing the production technology of chitin derivatives and enriching understanding of their biological functions will shed more light on their capability to improve human health.