bluuebunny/crossref_metadata_2025 huggingface dataset:
Geochemical Characterization and Mineralogical Assemblage of Burhi-Dihing and Noa-Dihing—The Largest South Bank Tributaries of the Brahmaputra River
Authors: Das Aparna , Handique Sumi , Chaowlu Monalisa , Kumar Manish
Published: October 2016

Mineralogical studies are valuable in understanding past weathering regimes induced by changing climatic conditions. Information about the bedrock lithology, weathering regimes, erosion and sedimentation rates are fundamental issues for better understanding of the river catchment behaviours. In this context, therefore, major ions, trace metals and clay mineral compositions of the sediment and water of Noa-Dihing and Burhi-Dihing rivers, the southbank tributaries of the Brahmaputra river, have been examined. Chemical index of alteration (CIA) values of both Noa-Dihing and Burhi-Dihing rivers suggests the prevalence of moderate chemical weathering events and formation of minerals such as muscovite, illite and smectite in the river catchment. Metal distribution in both rivers shows highly toxic metals, comprised of 32% of total mineral composition, and are less abundant with respect to moderately toxic metals (37%) except Zr, which comprises 37% and 32% respectively of the total mineral compositions. Comparison of sediment chemistry with composition of source rocks and average Upper Continental Crust (UCC) suggests higher depletion of CaO, Na2O, P2O5, Al2O3, SiO2, MgO and MnO signifying the dominance of mafic mineral phases. Loss of these metal oxides from the bed rocks during weathering and/or less abundance of clay in bed sediments compared to that in UCC may also be attributed to depleted values of these minerals. This is further substantiated by grain size analysis i.e. more abundance of sandy silt as compared to clay minerals in the overbank and channel sediments of Noa-Dihing and Burhi-Dihing rivers. Chemical Index of Alteration (CIA) varies significantly from 64.49 to 81.21, indicating large spatial variability in the intensity of chemical weathering of upper Brahmaputra basin. Multivariate analysis suggests that natural weathering processes of alkaline earth metals can be associated with release of trace metals in a riverine system and releasing mechanism of transition metal and their oxides are same and that of alkali and alkaline earth metal are similar.
Household Air Pollution from Cooking Fuels: An Environmental and Public Health Challenge
Authors: Joon Vinod , Chandra Avinash
Published: October 2016

Cooking is central to our lives. Nearly 40% of the world population and about half of population of developing countries of Asia, Africa and Latin America rely on biomass fuels for their cooking energy needs and 84% of them reside in rural areas. When used in simple cooking stoves, these fuels emit substantial amounts of toxic pollutants that include respirable particles, carbon monoxide, oxides of nitrogen and sulfur, benzene, formaldehyde, 1,3-butadiene, and poly aromatic compounds. In households with limited ventilation as is common in rural household of developing countries, exposures experienced by household members have been measured to be many times higher than World Health Organization’s indoor air quality guidelines. Due to customary role of woman in cooking, she is exposed more. As children are often carried on their mother’s back or lap while the cooking is in progress, they are also exposed to high levels of cooking smoke and therefore are at risk of getting respiratory problems.

Research studies give moderate to strong evidence linking household air pollution with chronic lung and other diseases making it a major public health problem of poor nations where it accounts for much of morbidity and over a million deaths annually. Also, fuel gathering adds time and transport burden on women and children, limiting their time for education and other productive work. In addition to health risks, incomplete combustion of biomass fuels are also responsible for greenhouse gas emissions which are the key environmental concern for climate change today.
Improvement of the Overall Biodegradability of Ciprofloxacin by Pre-treatment with Photocatalytic Oxidation of Wastewaters
Authors: Genç Nevim
Published: October 2016

In this study, photocatalytic degradation of ciprofloxacin (CIP) was investigated. CIP is a widely used quinolone antibiotic. Removal of CIP in the dark is negligible. The removal efficiency by photolysis of CIP was 25.3%. The removal efficiencies after 30 min irradiation were 85%, 93.5% and 97% at pH values 3, 6 and 9 in the presence of 0.5 g/L TiO2, respectively. Modified form of the Langmuir-Hinshelwood equation has been successful as a quantitive model to describe solid-liquid interactions. Kinetic constant k is reactivity constant, K is the adsorption equilibrium constant and kap (kK) is the apparent first order reaction constant, these were determined as 0.673 mg/L min, 0.662 L/mg and 0.445 (1/min), respectively. The results showed that after 30 min irradiation of CIP, solution was degraded 95%, whereas COD removed was 40.3%. The enhancement of biodegradability, evaluation in terms of the BOD5/COD ratio, increased from 0.043 to 0.403. This increase is not sufficient for treatment in conventional activated sludge unit. Additionally, the biodegradability of CIP photo-transformation products was assessed by BOD test. k (1/d), BOD reaction rate constants were determined as 0.0857 (1/d) and 0.1778 (1/d) for untreated and treated CIP solution, respectively.
Antitumor effects of iridomyrmecin in HeLa cervical cancer cells are mediated via apoptosis induction, loss of mitochondrial membrane potential, cell cycle arrest and down-regulation of PI3K/Akt and up-regulation of lncRNA CCAT2 expression
Authors: Lin Li , Cheng Xiao-Ling , Li Ming-Zhe , Wang Tao , Dong Min-Hai , Wang Zhen-Yu , Liao Min
Published: October 2016

The main purpose of the current study was to study the antitumor effects of iridomyrmecin against human cervical cancer cells (HeLa). Its effects on apoptosis induction, cell cycle phase distribution, PI3K/Akt signalling pathway and long non-coding RNA (lncRNA) were also investigated. Cytotoxic effects of iridomyrmecin were evaluated by MTT assay while the apoptotic effect was assessed by flow cytometry using annexin V-FITC assay. Western blot assay was used to study effects on PI3K/Akt signalling pathway. Results exhibited that iridomyrmecin led to concentration-dependent as well as time-dependent growth inhibitory effects. Iridomyrmecin-treated cells showed signs of early and late apoptosis. Iridomyrmecin treatment also led to sub-G1 cell cycle arrest as well as induced loss of mitochondrial membrane potential (ΔΨm). Further, Western blot assay revealed that iridomyrmecin treatment resulted in down-regulation of PI3K/Akt protein expressions in a dose-dependent manner while as it up-regulated lncRNA CCAT2 expression.

Video Clip:

Flow cytometry for cell cycle analysis: 3 min 20 sec

 
Reassessing the Romance: Tartan as a Popular Commodity, c. 1770–1830
Authors: Tuckett Sally
Published: October 2016

Through examining the surviving records of tartan manufacturers, William Wilson & Son of Bannockburn, this article looks at the production and use of tartan in the late eighteenth and early nineteenth centuries. While it does not deny the importance of the various meanings and interpretations attached to tartan since the mid-eighteenth century, this article contends that more practical reasons for tartan's popularity—primarily its functional and aesthetic qualities—merit greater attention. Along with evidence from contemporary newspapers and fashion manuals, this article focuses on evidence from the production and popular consumption of tartan at the turn of the nineteenth century, including its incorporation into fashionable dress and its use beyond the social elite. This article seeks to demonstrate the contemporary understanding of tartan as an attractive and useful commodity.
An Overview on the Applications of Matrix Theory in Wireless Communications and Signal Processing
Authors: Wang Xu , Serpedin Erchin
Published: October 2016

This paper overviews the key applications enabled by matrix theory in two major fields of interest in electrical engineering, namely wireless communications and signal processing. The paper focuses on the fundamental role played by matrices in modeling and optimization of wireless communication systems, and in detection, extraction and processing of the information embedded in signals. Among the major applications in wireless communications, the role of matrix representations and decompositions in characterizing multiple-input multiple-output (MIMO) and orthogonal frequency division multiplexing (OFDM) communication systems is described. In addition, this paper points out the important contribution made by matrices in solving signal estimation and detection problems. Special attention is given to the implementation of matrices in sensor array signal processing and the design of adaptive filters. Furthermore, the crucial role played by matrices in representing and processing digital images is depicted by several illustrative applications. This paper concludes with some applications of matrix theory in the area of compressive sensing of signals and by outlining a few open research problems for future study.
Benefits and Impact of Joint Metric of AOA/RSS/TOF on Indoor Localization Error
Authors: Jiang Qing , Qiu Feng , Zhou Mu , Tian Zengshan
Published: October 2016

The emerging techniques in the Fifth Generation (5G) communication system, like the millimeter-Wave (mmWave) and massive Multiple Input Multiple Output (MIMO), make it possible to measure the Angle-Of-arrival (AOA), Receive Signal Strength (RSS) and Time-Of-flight (TOF) by using various types of mobile devices. At the same time, there is always significant interest in the high-precision localization techniques based on the joint metric of AOA/RSS/TOF, which enable one to overcome the drawback of the single metric-based localization. Motivated by this concern, we rely on the Cramer–Rao Lower Bound (CRLB) to analyze the localization errors of RSS/AOA, RSS/TOF, AOA/TOF and the Joint Metric of AOA/RSS/TOF (JMART)-based localization. The error bounds derived in this paper can be selected as the benchmarking results to evaluate the indoor localization performance. Finally, extensive simulations are conducted to support our claim.
A Review on Predicting Ground PM2.5 Concentration Using Satellite Aerosol Optical Depth
Authors: Chu Yuanyuan , Liu Yisi , Li Xiangyu , Liu Zhiyong , Lu Hanson , Lu Yuanan , Mao Zongfu , Chen Xi , Li Na , Ren Meng , Liu Feifei , Tian Liqiao , Zhu Zhongmin , Xiang Hao
Published: October 2016

This study reviewed the prediction of fine particulate matter (PM2.5) from satellite aerosol optical depth (AOD) and summarized the advantages and limitations of these predicting models. A total of 116 articles were included from 1436 records retrieved. The number of such studies has been increasing since 2003. Among these studies, four predicting models were widely used: Multiple Linear Regression (MLR) (25 articles), Mixed-Effect Model (MEM) (23 articles), Chemical Transport Model (CTM) (16 articles) and Geographically Weighted Regression (GWR) (10 articles). We found that there is no so-called best model among them and each has both advantages and limitations. Regarding the prediction accuracy, MEM performs the best, while MLR performs worst. CTM predicts PM2.5 better on a global scale, while GWR tends to perform well on a regional level. Moreover, prediction performance can be significantly improved by combining meteorological variables with land use factors of each region, instead of only considering meteorological variables. In addition, MEM has advantages in dealing with the AOD data with missing values. We recommend that with the help of higher resolution AOD data, future works could be focused on developing satellite-based predicting models for the prediction of historical PM2.5 and other air pollutants.
Hypericin in the Dark: Foe or Ally in Photodynamic Therapy?
Authors: Huntosova Veronika , Stroffekova Katarina
Published: October 2016

Photosensitizers (PSs) in photodynamic therapy (PDT) are, in most cases, administered systemically with preferential accumulation in malignant tissues; however, exposure of non-malignant tissues to PS may also be clinically relevant, when PS molecules affect the pro-apoptotic cascade without illumination. Hypericin (Hyp) as PS and its derivatives have long been studied, regarding their photodynamic and photocytotoxic characteristics. Hyp and its derivatives have displayed light-activated antiproliferative and cytotoxic effects in many tumor cell lines without cytotoxicity in the dark. However, light-independent effects of Hyp have emerged. Contrary to the acclaimed Hyp minimal dark cytotoxicity and preferential accumulation in tumor cells, it was recently been shown that non-malignant and malignant cells uptake Hyp at a similar level. In addition, Hyp has displayed light-independent toxicity and anti-proliferative effects in a wide range of concentrations. There are multiple mechanisms underlying Hyp light-independent effects, and we are still missing many details about them. In this paper, we focus on Hyp light-independent effects at several sub-cellular levels—protein distribution and synthesis, organelle ultrastructure and function, and Hyp light-independent effects regarding reactive oxygen species (ROS). We summarize work from our laboratories and that of others to reveal an intricate network of the Hyp light-independent effects. We propose a schematic model of pro- and anti-apoptotic protein dynamics between cell organelles due to Hyp presence without illumination. Based on our model, Hyp can be explored as an adjuvant therapeutic drug in combination with chemo- or radiation cancer therapy.
Implications and Assessment of the Elastic Behavior of Lamins in Laminopathies
Authors: Dutta Subarna , Bhattacharyya Maitree , Sengupta Kaushik
Published: October 2016

Lamins are mechanosensitive and elastic components of the nuclear lamina that respond to external mechanical cues by altering gene regulation in a feedback mechanism. Numerous mutations in A-type lamins cause a plethora of diverse diseases collectively termed as laminopathies, the majority of which are characterized by irregularly shaped, fragile, and plastic nuclei. These nuclei are challenged to normal mechanotransduction and lead to disease phenotypes. Here, we review our current understanding of the nucleocytoskeleton coupling in mechanotransduction mediated by lamins. We also present an up-to-date understanding of the methods used to determine laminar elasticity both at the bulk and single molecule level.
Enhanced Thermoelectric Properties of Cu3SbSe3-Based Composites with Inclusion Phases
Authors: Liu Rui , Ren Guangkun , Tan Xing , Lin Yuanhua , Nan Cewen
Published: October 2016

Cu3SbSe3-based composites have been prepared by self-propagating high-temperature synthesis (SHS) combined with spark plasma sintering (SPS) technology. Phase composition and microstructure analysis indicate that the obtained samples are mainly composed of Cu3SbSe3 phase and CuSbSe2/Cu2−xSe secondary phases. Our results show that the existence of Cu2−xSe phase can clearly enhance the electrical conductivity of the composites (~16 S/cm), which is 2.5 times higher than the pure phase. The thermal conductivity can remain at about 0.30 W·m−1·K−1 at 653 K. A maximum ZT (defined as ZT = S2σΤ/κ, where S, σ, Τ, κ are the Seebeck coefficient, electrical conductivity, absolute temperature and total thermal conductivity) of the sample SPS 633 can be 0.42 at 653 K, which is 60% higher than the previously reported values. Our results indicate that the composite structure is an effective method to enhance the performance of Cu3SbSe3.
Temperature Field Accurate Modeling and Cooling Performance Evaluation of Direct-Drive Outer-Rotor Air-Cooling In-Wheel Motor
Authors: Chai Feng , Tang Yue , Pei Yulong , Liang Peixin , Gao Hongwei
Published: October 2016

High power density outer-rotor motors commonly use water or oil cooling. A reasonable thermal design for outer-rotor air-cooling motors can effectively enhance the power density without the fluid circulating device. Research on the heat dissipation mechanism of an outer-rotor air-cooling motor can provide guidelines for the selection of the suitable cooling mode and the design of the cooling structure. This study investigates the temperature field of the motor through computational fluid dynamics (CFD) and presents a method to overcome the difficulties in building an accurate temperature field model. The proposed method mainly includes two aspects: a new method for calculating the equivalent thermal conductivity (ETC) of the air-gap in the laminar state and an equivalent treatment to the thermal circuit that comprises a hub, shaft, and bearings. Using an outer-rotor air-cooling in-wheel motor as an example, the temperature field of this motor is calculated numerically using the proposed method; the results are experimentally verified. The heat transfer rate (HTR) of each cooling path is obtained using the numerical results and analytic formulas. The influences of the structural parameters on temperature increases and the HTR of each cooling path are analyzed. Thereafter, the overload capability of the motor is analyzed in various overload conditions.
Three-Year Study on Diurnal and Seasonal CO2 Sequestration of a Young Fraxinus griffithii Plantation in Southern Taiwan
Authors: Chen Chung-I , Wang Ya-Nan , Lih Hsueh-Wen , Yu Jui-Chu
Published: October 2016

This study examined monthly carbon sequestration of the Himalayan ash (Fraxinus griffithii C. B. Clarke), an important plantation species in Taiwan. From January 2010 to December 2012, data were collected from an F. griffithii plantation in southern Taiwan, which experiences a typical Southeast Asia monsoon climate. To estimate CO2 sequestration rate, we conducted diurnal measurements of photosynthetic rates and seasonal measurements of photosynthetic light response curves. We also calculated leaf area index to estimate the total leaf area of individual trees. The diurnal variation in photosynthetic rate, stomatal conductance, and transpiration exhibited seasonal and annual differences. The range of net CO2 assimilation rates was 1.34–8.68 µmol·m−2·s−1 in 2010, 1.02–6.60 µmol·m−2·s−1 in 2011, and 1.13–4.45 µmol·m−2·s−1 in 2012. A single F. griffithii tree sequestrated 12.21 kg·year−1 CO2 on average. Annual CO2 sequestration occurred primarily during the summer for all years, averaging 14.89 Mg·ha−1·year−1 for three years. Correlation analyses between various environmental variables and CO2 sequestration rates indicated that air temperature and soil water content were likely the main factors influencing carbon sequestration of F. griffithii at this study site.
Climatic Sensitivity of a Mixed Forest Association of White Spruce and Trembling Aspen at Their Southern Range Limit
Authors: Chhin Sophan , Wang G.
Published: October 2016

Climatic sensitivity of white spruce (Picea glauca (Moench) Voss) was examined growing in association with trembling aspen (Populus tremuloides Michx.) at their southern limit of distribution in a transitional ecotone between the southern boreal forest and northern prairie region. The study was carried out in the Spruce Woods Provincial Park (SWPP) located in southwestern Manitoba, Canada. The dry regional climate restricted trembling aspen growth during the growing season via moisture deficiency and temperature induced drought stress. Warm, mild winters also negatively affected radial growth of trembling aspen. Growth of white spruce was moderated by conditions within the aspen stands as radial growth patterns showed low variability from year to year, a low common growth signal, and a stronger response to temperature than to precipitation. Nonetheless, the dry regional climate still restricted growth of white spruce during the growing season via temperature induced drought stress. The findings of the study for white spruce support the stress gradient hypothesis in which facilitative interactions between tree species are expected under harsher environmental conditions.
Theoretical Study of the Effects of Magnetic Field Geometry on the High-Energy Emission of Blazars
Authors: Joshi Manasvita , Marscher Alan , Böttcher Markus
Published: October 2016

The knowledge of the structure of the magnetic field inside a blazar jet, as deduced from polarization observations at radio to optical wavelengths, is closely related to the formation and propagation of relativistic jets that result from accretion onto supermassive black holes. However, a largely unexplored aspect of the theoretical understanding of radiation transfer physics in blazar jets has been the magnetic field geometry as revealed by the polarized emission and the connection between the variability in polarization and flux across the spectrum. Here, we explore the effects of various magnetic geometries that can exist inside a blazar jet: parallel, oblique, toroidal, and tangled. We investigate the effects of changing the orientation of the magnetic field, according to the above-mentioned geometries, on the resulting high-energy spectral energy distributions (SEDs) and spectral variability patterns (SVPs) of a typical blazar. We use the MUlti-ZOne Radiation Feedback (MUZORF) model of Joshi et al. (2014) to carry out this study and to relate the geometry of the field to the observed SEDs at X-ray and γ-ray energies. One of the goals of the study is to understand the relationship between synchrotron and inverse Compton peaks in blazar SEDs and the reason for the appearance of γ-ray “orphan flares” observed in some blazars. This can be associated with the directionality of the magnetic field, which creates a difference in the radiation field as seen by an observer versus that seen by the electrons in the emission region.
The Influence of Crop Habitat and Control Strategies on Pepper Viruses in Andalusia (Spain)
Authors: Simón Almudena , García Carmen , Pascual Fernando , Ruiz Leticia , Janssen Dirk
Published: October 2016

Andalusia, southern Spain, is a major horticultural production region within the Mediterranean, where over 10,000 ha are dedicated to the production of pepper (Capsicum annuum L.). Approximately two-thirds of the area dedicated to this crop is in a greenhouse and the remaining one-third is comprised of open field crops. Using pepper as a model, we identified and compared the major diseases caused by viruses in the different geographic regions and agronomic systems within the region. Symptomatic samples were collected during 2009 and analyzed by ELISA and RT-PCR for the presence of Tomato spotted wilt virus (TSWV), Cucumber mosaic virus (CMV), Tomato mosaic virus (ToMV), Pepper mild mottle virus (PMMoV), Potato virus Y (PVY), Tobacco mild green mosaic virus (TMGMV), Tomato chlorosis virus (ToCV) and Parietaria mottle virus (PMoV). Contingency table analysis showed a significant relationship between the presence of major diseases caused by viruses in pepper crops and the different agrosystems in terms of location (inland versus coastal), disease control management (chemical versus integrated), cropping system (open field versus greenhouse), and virus-resistant versus susceptible cultivars. Pepper crops in plastic-covered greenhouses were predominantly associated with arthropod-transmitted virus diseases, such as TSWV. CMV was predominant in provinces located inland, and PMoV was found independent of the agrosystem, disease control methods, or geographic location.
Protection from Ultraviolet Radiation during Childhood: The Parental Perspective in Bavaria
Authors: Gefeller Olaf , Uter Wolfgang , Pfahlberg Annette
Published: October 2016

During childhood, parents play a vital role in sun protection of their children. Their guidance is essential for avoiding excessive exposure to ultraviolet (UV) radiation, a risk factor for developing skin cancer in later life. In a population-based cross-sectional study conducted between October 2011 and February 2012, we assessed how 3281 parents implemented sun protection for their three- to six-year-old children in practice. In particular, clothing, shade-seeking behavior, wearing of sunhats and sunglasses, use of sunscreens and the amount of time spent outdoors were ascertained in two settings (beach, garden/playground). The results showed that the overall level of parental sun protection for their children in the beach setting, and to a lesser extent also in the everyday outdoor setting, is relatively high. Using sunscreens with a high sun protection factor and instructing children to wear a sunhat were very common. Lesser attention was paid to sun-protective clothing, seeking the shade and wearing sunglasses. The amount of time spent outdoors during summer days was high. Therefore, the recommendation to completely avoid sun exposure during peak UV times around noon during summertime needs to be reinforced. In addition, the observed difference in the protective behavior between the beach and an everyday outdoor setting points to the necessity to encourage better sun protection for children also in outdoor activities of daily living.
Development of an Advanced HPLC–MS/MS Method for the Determination of Carotenoids and Fat-Soluble Vitamins in Human Plasma
Authors: Hrvolová Barbora , Martínez-Huélamo Miriam , Colmán-Martínez Mariel , Hurtado-Barroso Sara , Lamuela-Raventós Rosa , Kalina Jiří
Published: October 2016

The concentration of carotenoids and fat-soluble vitamins in human plasma may play a significant role in numerous chronic diseases such as age-related macular degeneration and some types of cancer. Although these compounds are of utmost interest for human health, methods for their simultaneous determination are scarce. A new high pressure liquid chromatography (HPLC)-tandem mass spectrometry (MS/MS) method for the quantification of selected carotenoids and fat-soluble vitamins in human plasma was developed, validated, and then applied in a pilot dietary intervention study with healthy volunteers. In 50 min, 16 analytes were separated with an excellent resolution and suitable MS signal intensity. The proposed HPLC–MS/MS method led to improvements in the limits of detection (LOD) and quantification (LOQ) for all analyzed compounds compared to the most often used HPLC–DAD methods, in some cases being more than 100-fold lower. LOD values were between 0.001 and 0.422 µg/mL and LOQ values ranged from 0.003 to 1.406 µg/mL, according to the analyte. The accuracy, precision, and stability met with the acceptance criteria of the AOAC (Association of Official Analytical Chemists) International. According to these results, the described HPLC-MS/MS method is adequately sensitive, repeatable and suitable for the large-scale analysis of compounds in biological fluids.
A Survey on Data Compression Methods for Biological Sequences
Authors: Hosseini Morteza , Pratas Diogo , Pinho Armando
Published: October 2016

The ever increasing growth of the production of high-throughput sequencing data poses a serious challenge to the storage, processing and transmission of these data. As frequently stated, it is a data deluge. Compression is essential to address this challenge—it reduces storage space and processing costs, along with speeding up data transmission. In this paper, we provide a comprehensive survey of existing compression approaches, that are specialized for biological data, including protein and DNA sequences. Also, we devote an important part of the paper to the approaches proposed for the compression of different file formats, such as FASTA, as well as FASTQ and SAM/BAM, which contain quality scores and metadata, in addition to the biological sequences. Then, we present a comparison of the performance of several methods, in terms of compression ratio, memory usage and compression/decompression time. Finally, we present some suggestions for future research on biological data compression.
Modeling Mental Speed: Decomposing Response Time Distributions in Elementary Cognitive Tasks and Correlations with Working Memory Capacity and Fluid Intelligence
Authors: Schmitz Florian , Wilhelm Oliver
Published: October 2016

Previous research has shown an inverse relation between response times in elementary cognitive tasks and intelligence, but findings are inconsistent as to which is the most informative score. We conducted a study (N = 200) using a battery of elementary cognitive tasks, working memory capacity (WMC) paradigms, and a test of fluid intelligence (gf). Frequently used candidate scores and model parameters derived from the response time (RT) distribution were tested. Results confirmed a clear correlation of mean RT with WMC and to a lesser degree with gf. Highly comparable correlations were obtained for alternative location measures with or without extreme value treatment. Moderate correlations were found as well for scores of RT variability, but they were not as strong as for mean RT. Additionally, there was a trend towards higher correlations for slow RT bands, as compared to faster RT bands. Clearer evidence was obtained in an ex-Gaussian decomposition of the response times: the exponential component was selectively related to WMC and gf in easy tasks, while mean response time was additionally predictive in the most complex tasks. The diffusion model parsimoniously accounted for these effects in terms of individual differences in drift rate. Finally, correlations of model parameters as trait-like dispositions were investigated across different tasks, by correlating parameters of the diffusion and the ex-Gaussian model with conventional RT and accuracy scores.
Cell Migration According to Shape of Graphene Oxide Micropatterns
Authors: Kim Sung , Kim Min , Shin Yong , Eom Seong , Lee Jong , Shin Dong-Myeong , Hong Suck , Kim Bongju , Park Jong-Chul , Shin Bo , Lim Dohyung , Han Dong-Wook
Published: October 2016

Photolithography is a unique process that can effectively manufacture micro/nano-sized patterns on various substrates. On the other hand, the meniscus-dragging deposition (MDD) process can produce a uniform surface of the substrate. Graphene oxide (GO) is the oxidized form of graphene that has high hydrophilicity and protein absorption. It is widely used in biomedical fields such as drug delivery, regenerative medicine, and tissue engineering. Herein, we fabricated uniform GO micropatterns via MDD and photolithography. The physicochemical properties of the GO micropatterns were characterized by atomic force microscopy (AFM), scanning electron microscopy (SEM), and Raman spectroscopy. Furthermore, cell migration on the GO micropatterns was investigated, and the difference in cell migration on triangle and square GO micropatterns was examined for their effects on cell migration. Our results demonstrated that the GO micropatterns with a desired shape can be finely fabricated via MDD and photolithography. Moreover, it was revealed that the shape of GO micropatterns plays a crucial role in cell migration distance, speed, and directionality. Therefore, our findings suggest that the GO micropatterns can serve as a promising biofunctional platform and cell-guiding substrate for applications to bioelectric devices, cell-on-a-chip, and tissue engineering scaffolds.
The Electroluminescence Mechanism of Solution-Processed TADF Emitter 4CzIPN Doped OLEDs Investigated by Transient Measurements
Authors: Wang Peng , Zhao Suling , Xu Zheng , Qiao Bo , Long Zhijuan , Huang Qingyu
Published: October 2016

High efficiency, solution-processed, organic light emitting devices (OLEDs), using a thermally-activated delayed fluorescent (TADF) emitter, 1,2,3,5-tetrakis(carbazol-9-yl)-4,6-dicyanobenzene (4CzIPN), are fabricated, and the transient electroluminescence (EL) decay of the device with a structure of [ITO/PEDOT: PSS/4CzIPN 5 wt % doped 4,40-N,N0-dicarbazolylbiphenyl(CBP)/bis-4,6-(3,5-di-4-pyridylphenyl)-2-methylpyrimidine (B4PyMPM)/lithium fluoride (LiF)/Al], is systematically studied. The results shed light on the dominant operating mechanism in TADF-based OLEDs. Electroluminescence in the host–guest system is mainly produced from the 4CzIPN emitter, rather than the exciplex host materials.
Contribution of Water from Food and Fluids to Total Water Intake: Analysis of a French and UK Population Surveys
Authors: Guelinckx Isabelle , Tavoularis Gabriel , König Jürgen , Morin Clémentine , Gharbi Hakam , Gandy Joan
Published: October 2016

Little has been published on the contribution of food moisture (FM) to total water intake (TWI); therefore, the European Food Safety Authority assumed FM to contribute 20%–30% to TWI. The aim of the present analysis was to estimate and compare TWI, the percentage of water from FM and from fluids in population samples of France and UK. Data from 2 national nutrition surveys (Enquête Comportements et Consommations Alimentaires en France (CCAF) 2013 and the National Diet and Nutrition Survey (NDNS) 2008/2009–2011/2012) were analyzed for TWI and the contribution of water from FM and fluids. Children and adults TWI were significantly lower in France than in the UK. The contribution of water from foods was lower in the UK than in France (27% vs. 36%). As TWI increased, the proportion of water from fluids increased, suggesting that low drinkers did not compensate by increasing intake of water-rich foods. In addition, 80%–90% of the variance in TWI was explained by differences in water intake from fluids. More data on the contribution of FM to TWI is needed to develop more robust dietary recommendations on TWI and guidance on fluid intake for the general public.
Paradoxical Effects of Fruit on Obesity
Authors: Sharma Satya , Chung Hea , Kim Hyeon , Hong Seong
Published: October 2016

Obesity is exponentially increasing regardless of its preventable characteristics. The current measures for preventing obesity have failed to address the severity and prevalence of obesity, so alternative approaches based on nutritional and diet changes are attracting attention for the treatment of obesity. Fruit contains large amounts of simple sugars (glucose, fructose, sucrose, etc.), which are well known to induce obesity. Thus, considering the amount of simple sugars found in fruit, it is reasonable to expect that their consumption should contribute to obesity rather than weight reduction. However, epidemiological research has consistently shown that most types of fruit have anti-obesity effects. Thus, due to their anti-obesity effects as well as their vitamin and mineral contents, health organizations are suggesting the consumption of fruit for weight reduction purposes. These contradictory characteristics of fruit with respect to human body weight management motivated us to study previous research to understand the contribution of different types of fruit to weight management. In this review article, we analyze and discuss the relationships between fruit and their anti-obesity effects based on numerous possible underlying mechanisms, and we conclude that each type of fruit has different effects on body weight.
Diet Quality—The Greeks Had It Right!
Authors: Anderson John , Nieman David
Published: October 2016

The Mediterranean diet is upheld in the 2015–2020 Dietary Guidelines as an example of an eating pattern that promotes good health, a healthy body weight, and disease prevention throughout the lifespan. The Mediterranean eating pattern is based on a variety of unprocessed plant foods including fruits, vegetables, whole grains, legumes, nuts, and seeds that are high in polyphenols. The majority of polyphenols arrive in the colon where bacteria degrade them into smaller phenolics that can be translocated via the portal vein to the liver. In the liver, the phenolics undergo additional biotransformation prior to release into the circulation and transport to specific tissues where bioactive effects take place before removal in the urine. Recent epidemiologic studies using improved assessment techniques support that high versus low dietary polyphenol intake predicts reduced risk for neurodegenerative diseases, diabetes, cardiovascular disease, hypertension, obesity, and early death from all causes. Emerging science reveals that many of these health-related benefits can be traced to the biotransformed, gut-derived phenolics. In conclusion, the high consumption of unprocessed plant foods by inhabitants of countries bordering the Mediterranean Sea has been linked to multiple health and disease prevention benefits that are in large part due to a varied intake of polyphenols.
The Identification of Biochanin A as a Potent and Selective β-Site App-Cleaving Enzyme 1 (Bace1) Inhibitor
Authors: Youn Kumju , Park Ji-Hyun , Lee Jinhyuk , Jeong Woo-Sik , Ho Chi-Tang , Jun Mira
Published: October 2016

Beta-site amyloid precursor protein cleaving enzyme 1 (BACE1) is the enzyme involved in the abnormal production of the amyloidogenic peptide Aβ, one of the major causes of histological hallmarks of Alzheimer’s disease (AD). Thus, BACE1 represents a key target protein in the development of new potential target for the prevention and treatment of AD. In this study, in vitro anti-AD activity of biochanin A, a dietary isoflavone found in legumes and most notably red clover, were evaluated via human recombinant BACE1 inhibition assay, as well as enzyme kinetic and molecular docking predictions. Enzyme-based assays revealed that biochanin A exhibited a non-competitive inhibitory effect on BACE1 with an IC50 value of 28 μM and a Ki of 43 μM. In addition, docking simulation results demonstrated that ASN37, SER35, SER36, TRP76, and ARG128 residues of BACE1 interacted with biochanin A. Moreover, the binding energy of biochanin A was negative (−8.4 kcal/mol), indicating that it might potentiate a strong binding between the compound and the allosteric site of BACE1, resulting in further effective BACE1 inhibition. The present novel findings raise the possibility that biochanin A may be used as a preventative, developed into a therapeutic agent for AD, or both.
Effect of Green Tea Extract on Systemic Metabolic Homeostasis in Diet-Induced Obese Mice Determined via RNA-Seq Transcriptome Profiles
Authors: Choi Ji-Young , Kim Ye , Ryu Ri , Cho Su-Jung , Kwon Eun-Young , Choi Myung-Sook
Published: October 2016

Green tea (GT) has various health effects, including anti-obesity properties. However, the multiple molecular mechanisms of the effects have not been fully determined. The aim of this study was to elucidate the anti-obesity effects of GT via the analysis of its metabolic and transcriptional responses based on RNA-seq profiles. C57BL/6J mice were fed a normal, high-fat (60% energy as fat), or high-fat + 0.25% (w/w) GT diet for 12 weeks. The GT extract ameliorated obesity, hepatic steatosis, dyslipidemia, and insulin resistance in diet-induced obesity (DIO) mice. GT supplementation resulted in body weight gain reduction than mice fed high-fat through enhanced energy expenditure, and reduced adiposity. The transcriptome profiles of epididymal white adipose tissue (eWAT) suggested that GT augments transcriptional responses to the degradation of branched chain amino acids (BCAAs), as well as AMP-activated protein kinase (AMPK) signaling, which suggests enhanced energy homeostasis. Our findings provide some significant insights into the effects of GT for the prevention of obesity and its comorbidities. We demonstrated that the GT extract contributed to the regulation of systemic metabolic homeostasis via transcriptional responses to not only lipid and glucose metabolism, but also amino acid metabolism via BCAA degradation in the adipose tissue of DIO mice.
Atomistic Modelling of Confined Polypropylene Chains between Ferric Oxide Substrates at Melt Temperature
Authors: Gooneie Ali , Gonzalez-Gutierrez Joamin , Holzer Clemens
Published: October 2016

The interactions and conformational characteristics of confined molten polypropylene (PP) chains between ferric oxide (Fe2O3) substrates were investigated by molecular dynamics (MD) simulations. A comparative analysis of the adsorbed amount shows strong adsorption of the chains on the high-energy surface of Fe2O3. Local structures formed in the polymer film were studied utilizing density profiles, orientation of bonds, and end-to-end distance of chains. At interfacial regions, the backbone carbon-carbon bonds of the chains preferably orient in the direction parallel to the surface while the carbon-carbon bonds with the side groups show a slight tendency to orient normal to the surface. Based on the conformation tensor data, the chains are compressed in the normal direction to the substrates in the interfacial regions while they tend to flatten in parallel planes with respect to the surfaces. The orientation of the bonds as well as the overall flattening of the chains in planes parallel to the solid surfaces are almost identical to that of the unconfined PP chains. Also, the local pressure tensor is anisotropic closer to the solid surfaces of Fe2O3 indicating the influence of the confinement on the buildup imbalance of normal and tangential pressures.
Relaxation Oscillation with Picosecond Spikes in a Conjugated Polymer Laser
Authors: Mujamammi Wafa , Prasad Saradh , AlSalhi Mohamad , Masilamani Vadivel
Published: October 2016

Optically pumped conjugated polymer lasers are good competitors for dye lasers, often complementing and occasionally replacing them. This new type of laser material has broad bandwidths and high optical gains comparable to conventional laser dyes. Since the Stokes’ shift is unusually large, the conjugated polymer has a potential for high power laser action, facilitated by high concentration. This paper reports the results of a new conjugated polymer, the poly[(9,9-dioctyl-2,7-divinylenefluorenylene)-alt-co-{2-methoxy-5-(2-ethylhexyloxy)-1,4-phenylene}](PFO-co-MEH-PPV) material, working in the green region. Also discussed are the spectral and temporal features of the amplified spontaneous emissions (ASE) from the conjugated polymer PFO-co-MEH-PPV in a few solvents. When pumped by the third harmonic of the Nd:YAG laser of 10 ns pulse width, the time-resolved spectra of the ASE show relaxation oscillations and spikes of 600 ps pulses. To the best of our knowledge, this is the first report on relaxation oscillations in conjugated-polymer lasers.
Global Proteome Changes in Liver Tissue 6 Weeks after FOLFOX Treatment of Colorectal Cancer Liver Metastases
Authors: Urdzik Jozef , Vildhede Anna , Wiśniewski Jacek , Duraj Frans , Haglund Ulf , Artursson Per , Norén Agneta
Published: October 2016

(1) Oxaliplatin-based chemotherapy for colorectal cancer liver metastasis is associated with sinusoidal injury of liver parenchyma. The effects of oxaliplatin-induced liver injury on the protein level remain unknown. (2) Protein expression in liver tissue was analyzed—from eight patients treated with FOLFOX (combination of fluorouracil, leucovorin, and oxaliplatin) and seven controls—by label-free liquid chromatography mass spectrometry. Recursive feature elimination–support vector machine and Welch t-test were used to identify classifying and relevantly changed proteins, respectively. Resulting proteins were analyzed for associations with gene ontology categories and pathways. (3) A total of 5891 proteins were detected. A set of 184 (3.1%) proteins classified the groups with a 20% error rate, but relevant change was observed only in 55 (0.9%) proteins. The classifying proteins were associated with changes in DNA replication (p < 0.05) through upregulation of the minichromosome maintenance complex and with the innate immune response (p < 0.05). The importance of DNA replication changes was supported by the results of Welch t-test (p < 0.05). (4) Six weeks after FOLFOX treatment, less than 1% of identified proteins showed changes in expression associated with DNA replication, cell cycle entry, and innate immune response. We hypothesize that the changes remain after recovery from FOLFOX treatment injury.
Application and Uses of Electronic Noses for Clinical Diagnosis on Urine Samples: A Review
Authors: Capelli Laura , Taverna Gianluigi , Bellini Alessia , Eusebio Lidia , Buffi Niccolò , Lazzeri Massimo , Guazzoni Giorgio , Bozzini Giorgio , Seveso Mauro , Mandressi Alberto , Tidu Lorenzo , Grizzi Fabio , Sardella Paolo , Latorre Giuseppe , Hurle Rodolfo , Lughezzani Giovanni , Casale Paolo , Meregali Sara , Sironi Selena
Published: October 2016

The electronic nose is able to provide useful information through the analysis of the volatile organic compounds in body fluids, such as exhaled breath, urine and blood. This paper focuses on the review of electronic nose studies and applications in the specific field of medical diagnostics based on the analysis of the gaseous headspace of human urine, in order to provide a broad overview of the state of the art and thus enhance future developments in this field. The research in this field is rather recent and still in progress, and there are several aspects that need to be investigated more into depth, not only to develop and improve specific electronic noses for different diseases, but also with the aim to discover and analyse the connections between specific diseases and the body fluids odour. Further research is needed to improve the results obtained up to now; the development of new sensors and data processing methods should lead to greater diagnostic accuracy thus making the electronic nose an effective tool for early detection of different kinds of diseases, ranging from infections to tumours or exposure to toxic agents.
A Low-Power All-Digital on-Chip CMOS Oscillator for a Wireless Sensor Node
Authors: Sheng Duo , Hong Min-Rong
Published: October 2016

This paper presents an all-digital low-power oscillator for reference clocks in wireless body area network (WBAN) applications. The proposed on-chip complementary metal-oxide-semiconductor (CMOS) oscillator provides low-frequency clock signals with low power consumption, high delay resolution, and low circuit complexity. The cascade-stage structure of the proposed design simultaneously achieves high resolution and a wide frequency range. The proposed hysteresis delay cell further reduces the power consumption and hardware costs by 92.4% and 70.4%, respectively, relative to conventional designs. The proposed design is implemented in a standard performance 0.18 μm CMOS process. The measured operational frequency ranged from 7 to 155 MHz, and the power consumption was improved to 79.6 μW (@7 MHz) with a 4.6 ps resolution. The proposed design can be implemented in an all-digital manner, which is highly desirable for system-level integration.