bluuebunny/crossref_metadata_2025 huggingface dataset:
Relationship between demographic and social variables and performance in virtual reality among healthcare personnel: an observational study
Authors: Katz Daniel , Hyers Benjamin , Patten Eric , Sarte Darren , Loo Mariano , Burnett Garrett W.
Published: March 2024

Background

Virtual reality is emerging as an important component of medical education. Although the benefits of virtual reality are apparent, the optimal strategy to orient to or differentiate learners in the virtual space have not been delineated. The purpose of this study was to investigate the relationships between demographic variables, social variables, and self-perceived comfort with technology to performance on a standardized non-medical virtual reality experience.

Methods

This observational study was performed at the International Meeting on Simulation in Healthcare in 2022. This conference includes medical and non-medical attendees. Participants provided demographic information and participated in a scored non-medical VR experience due to the heterogeneity of the sample. Participants then completed a System Usability Index and NASA Task Load Index form. Participants were dividedintolow scoring, medium scoring, and high scoring groups according to their final game score for further analysis.

Results

95 participants were included in final analysis. 55 (57.9%) of participants had prior virtual reality experience. Higher scores were associated with younger age (11.09, p < 0.001), identifying as male (11.09, p < 0.001), and a higher frequency of playing video games in the past (18.96, p < 0.001). The high score group was more likely to report comfort with virtual reality (6.29, p = 0.003) as well as comfort with new technology (4.61, p = 0.012). NASA Task Load Index scores trended down and System Usability Index scores trended up with increasing score. Being a nurse was a positive predictor of a higher score when compared to physicians in the multivariate analysis.

Conclusion

Performance during an immersive virtual reality experience was most closely related to age, gender, and frequency of playing video games. Self-perceived comfort with virtual reality was more predictive of score than prior virtual reality experience.
Assessment of the effectiveness of the BOPPPS model combined with case-based learning on nursing residency education for newly recruited nurses in China: a mixed methods study
Authors: Wang Yongli , Chen Yiqian , Wang Ling , Wang Wen , Kong Xiangyan , Li Xiaodan
Published: March 2024

Background

Expanding new nurse training and education is a priority for nursing educators as well as a critical initiative to stabilize the nursing workforce. Given that there is currently no standardized program for the training of new nurses in China, we investigated the effectiveness of the bridge-in, objective, pre-assessment, participatory learning, post-assessment, and summary model combined with case-based learning ((BOPPPS-CBL) for the standardized training of new nurses.

Methods

The mixed method approach with explanatory sequential (quantitative-qualitative) method was used. A questionnaire was used to compare the impact of the BOPPPS-CBL model and the Traditional Learning Model (TLM) on the core competencies of 185 new nurses for two years of standardized training. Quantitative data were analyzed using SPSS 22.0. Focus group interviews were used with four groups of new nurses and perceptions of BOPPPS-CBL training were recorded. Qualitative data were analyzed thematically.

Results

According to the quantitative data, more new nurses agreed that the BOPPPS-CBL model stimulated their learning and improved their core nursing competencies than the TLM. The BOPPPS-CBL group outperformed the TLM group on theoretical knowledge tests. Qualitative data revealed that 87.5% of new nurses agreed on the value of BOPPPS-CBL training, and three themes were extracted: (1) role promotion; (2) formation of new thinking to solve clinical problems; and (3) suggestions for improvement.

Conclusion

BOPPPS-CBL training had a significant impact on improving new nurses’ core competencies and promoting the transition of new nurses to clinical practice nurses in China. The study recommends BOPPPS-CBL training as an effective teaching model for the standardized training and education of new nurses.
Left ventricular and atrial myocardial strain in heart failure with preserved ejection fraction: the evidence so far and prospects for phenotyping strategy
Authors: Shinzato Mariane Higa , Santos Natasha , Nishida Gustavo , Moriya Henrique , Assef Jorge , Feres Fausto , Hortegal Renato A.
Published: March 2024

Background

Heart failure with preserved ejection fraction (HFpEF) represents a significant proportion of heart failure cases. Accurate diagnosis is challenging due to the heterogeneous nature of the disease and limitations in traditional echocardiographic parameters.

Main body

This review appraises the application of Global Longitudinal Strain (GLS) and Left Atrial Strain (LAS) as echocardiographic biomarkers in the diagnosis and phenotyping of HFpEF. Strain imaging, particularly Speckle Tracking Echocardiography, offers a superior assessment of myocardial deformation, providing a more detailed insight into left heart function than traditional metrics. Normal ranges for GLS and LAS are considered, acknowledging the impact of demographic and technical factors on these values. Clinical studies have demonstrated the prognostic value of GLS and LAS in HFpEF, especially in predicting cardiovascular outcomes and distinguishing HFpEF from other causes of dyspnea. Nevertheless, the variability of strain measurements and the potential for false-negative results underline the need for careful clinical interpretation. The HFA-PEFF scoring system's integration of these biomarkers, although systematic, reveals gaps in addressing the full spectrum of HFpEF pathology. The combined use of GLS and LAS has been suggested to define HFpEF phenogroups, which could lead to more personalized treatment plans.

Conclusion

GLS and LAS have emerged as pivotal tools in the non-invasive diagnosis and stratification of HFpEF, offering a promise for tailored therapeutic strategies. Despite their potential, a structured approach to incorporating these biomarkers into standard diagnostic workflows is essential. Future clinical guidelines should include clear directives for the combined utilization of GLS and LAS, accentuating their role in the multidimensional assessment of HFpEF.
RETRACTED ARTICLE: An overview on mRNA-based vaccines to prevent monkeypox infection
Authors: Natami Mohammad , Gorgzadeh Amirsasan , Gholipour Arsalan , Fatemi Seyedeh Narges , Firouzeh Nima , Zokaei Maryam , Mohammed Ali Saad Hasan , Kheradjoo Hadis , Sedighi Somayeh , Gholizadeh Omid , Kalavi Shaylan
Published: March 2024

The human monkeypox virus (Mpox) is classified as a member of the Poxviridae family and belongs to the Orthopoxvirus genus. Mpox possesses double-stranded DNA, and there are two known genetic clades: those originating in West Africa and the Congo Basin, commonly known as Central African clades. Mpox may be treated with either the vaccinia vaccination or the therapeutics. Modifying the smallpox vaccine for treating and preventing Mpox has shown to be beneficial because of the strong link between smallpox and Mpox viruses and their categorization in the same family. Cross-protection against Mpox is effective with two Food and Drug Administration (FDA)-approved smallpox vaccines (ACAM2000 and JYNNEOSTM). However, ACAM2000 has the potential for significant adverse effects, such as cardiac issues, whereas JYNNEOS has a lower risk profile. Moreover, Mpox has managed to resurface, although with modified characteristics, due to the discontinuation and cessation of the smallpox vaccine for 40 years. The safety and efficacy of the two leading mRNA vaccines against SARS-CoV-2 and its many variants have been shown in clinical trials and subsequent data analysis. This first mRNA treatment model involves injecting patients with messenger RNA to produce target proteins and elicit an immunological response. High potency, the possibility of safe administration, low-cost manufacture, and quick development is just a few of the benefits of RNA-based vaccines that pave the way for a viable alternative to conventional vaccines. When protecting against Mpox infection, mRNA vaccines are pretty efficient and may one day replace the present whole-virus vaccines. Therefore, the purpose of this article is to provide a synopsis of the ongoing research, development, and testing of an mRNA vaccine against Mpox.
Application of hand-sewn esophagojejunostomy in laparoscopic total gastrectomy
Authors: Gu Hao , Li Weixiang , Zhou Lianbang
Published: March 2024

Objective

To investigate the clinical efficacy and prognostic implication of hand-sewn anastomosis in laparoscopic total gastrectomy (LTG).

Methods

Retrospective analysis is adopted to the clinicopathologic data of 112 patients with gastric cancer (GC) who went through LTG in the Department of General Surgery, the Second Affiliated Hospital of Anhui Medical University between October 2020 and October 2022. Among them, 60 individuals receiving medical care were split into the hand-sewn anastomosis group (Group H, N = 60); while, 52 individuals were split into the circular stapler anastomosis group (Group C, N = 52) The clinical efficacy and prognostic conditions of hand-sewn anastomosis are compared with those of circular stapler anastomosis in the application of LTG.

Results

The analysis results indicated that no notable difference was observed in intraoperative bleeding volume, time to first flatus (TFF), postoperative hospitalization duration and postoperative complications among the two groups (P > 0.05). Group H had shorter esophagojejunal anastomosis duration (20.0 min vs. 35.0 min) and surgery duration (252.6 ± 19.4 min vs. 265.9 ± 19.8 min), smaller incisions (5.0 cm vs. 10.5 cm), and lower hospitalization costs (58415.0 CNY vs. 63382.5 CNY) compared to Group C (P < 0.05).

Conclusion

The clinical efficacy and the postoperative complications of hand-sewn esophagojejunostomy are basically equivalent in comparison to the circular stapler anastomosis in the application of LTG. Its advantage lies in shorter esophagojejunal anastomosis duration, shorter surgery duration, smaller incisions, lower hospitalization costs and wider adaptability of the location of the tumor.
A novel network pharmacology strategy to decode mechanism of Wuling Powder in treating liver cirrhosis
Authors: Liu Qinwen , Li Xiaowei , Li Yi , Luo Qian , Fan Qiling , Lu Aiping , Guan Daogang , Li Jiahui
Published: March 2024

Background

Liver cirrhosis is a chronic liver disease with hepatocyte necrosis and lesion. As one of the TCM formulas Wuling Powder (WLP) is widely used in the treatment of liver cirrhosis. However, it’s key functional components and action mechanism still remain unclear. We attempted to explore the Key Group of Effective Components (KGEC) of WLP in the treatment of Liver cirrhosis through integrative pharmacology combined with experiments.

Methods

The components and potential target genes of WLP were extracted from published databases. A novel node importance calculation model considering both node control force and node bridging force is designed to construct the Function Response Space (FRS) and obtain key effector proteins. The genetic knapsack algorithm was employed to select KGEC. The effectiveness and reliability of KGEC were evaluated at the functional level by using gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. Finally, the effectiveness and potential mechanism of KGEC were confirmed by CCK-8, qPCR and Western blot.

Results

940 effective proteins were obtained in FRS. KEGG pathways and GO terms enrichments analysis suggested that effective proteins well reflect liver cirrhosis characteristics at the functional level. 29 components of WLP were defined as KGEC, which covered 100% of the targets of the effective proteins. Additionally, the pathways enriched for the KGEC targets accounted for 83.33% of the shared genes between the targets and the pathogenic genes enrichment pathways. Three components scopoletin, caryophyllene oxide, and hydroxyzinamic acid from KGEC were selected for in vivo verification. The qPCR results demonstrated that all three components significantly reduced the mRNA levels of COL1A1 in TGF-β1-induced liver cirrhosis model. Furthermore, the Western blot assay indicated that these components acted synergistically to target the NF-κB, AMPK/p38, cAMP, and PI3K/AKT pathways, thus inhibiting the progression of liver cirrhosis.

Conclusion

In summary, we have developed a new model that reveals the key components and potential mechanisms of WLP for the treatment of liver cirrhosis. This model provides a reference for the secondary development of WLP and offers a methodological strategy for studying TCM formulas.
Implementation of lockdown, quarantine, and isolation measures in the context of COVID-19 among internally displaced persons in Burkina Faso: a qualitative study
Authors: Kadio Kadidiatou , Ly Antarou , Ouédraogo Adidjata , Ahmed Mohamed Ali Ag , Yaya Sanni , Gagnon Marie-Pierre
Published: March 2024

Background

The triple political, security, and health crisis in Burkina Faso has impacted the lives of Burkinabè people, resulting in massive internal displacement. These internally displaced persons (IDPs) are very vulnerable to epidemic diseases, which was exacerbated by the recent COVID-19 pandemic., The implementation of public health measures to curb the spread of COVID-19 represented a major concern among IDPs. The objective of this study was to document knowledge, difficulties, adjustments, and challenges faced by IDPs and humanitarian authorities/actors during implementation of lockdown, quarantine, and isolation measures in response to COVID-19.

Methods

The study was conducted in Burkina Faso, in the north-central region Kaya, a commune which hosts the largest number of IDPs in the country. Qualitative research using semi-structured interviews collected discursive data from 18 authorities and/or humanitarian actors and 29 IDPs in June 2021. The transcribed interviews were coded with N’vivo 11 software and analyzed thematically.

Results

Although respondents had a good knowledge of lockdown, isolation, and quarantine measures, the difference between these three concepts was not easily understood by either authorities/humanitarian actors or IDPs. Communication was one of the biggest challenges for humanitarian actors. The difficulties encountered by IDPs were economic (lack of financial resources), infrastructural (limited housing), and socio-cultural in the application of lockdown, isolation, and quarantine measures. As for adjustment measures, the health authorities developed a strategy for isolation and quarantine for the management of positive and suspected cases. The IDPs mentioned their commitment to compliance and awareness of lockdown measures as the main adjustment.

Conclusion

Although there were no known cases of COVID-19 among the IDPs at the time of the study, tailored response plans were developed to facilitate the application of these measures in emergencies. The involvement of IDPs in the communication and sensitization process was necessary to facilitate their adherence to these different measures.
Tight junction protein cingulin variant is associated with cancer susceptibility by overexpressed IQGAP1 and Rac1-dependent epithelial-mesenchymal transition
Authors: Huang Yi-Ting , Hsu Ya-Ting , Wu Pei-Ying , Yeh Yu-Min , Lin Peng-Chan , Hsu Keng-Fu , Shen Meng-Ru
Published: March 2024

Background

Cingulin (CGN) is a pivotal cytoskeletal adaptor protein located at tight junctions. This study investigates the link between CGN mutation and increased cancer susceptibility through genetic and mechanistic analyses and proposes a potential targeted therapeutic approach.

Methods

In a high-cancer-density family without known pathogenic variants, we performed tumor-targeted and germline whole-genome sequencing to identify novel cancer-associated variants. Subsequently, these variants were validated in a 222 cancer patient cohort, and CGN c.3560C > T was identified as a potential cancer-risk allele. Both wild-type (WT) (c.3560C > C) and variant (c.3560C > T) were transfected into cancer cell lines and incorporated into orthotopic xenograft mice model for evaluating their effects on cancer progression. Western blot, immunofluorescence analysis, migration and invasion assays, two-dimensional gel electrophoresis with mass spectrometry, immunoprecipitation assays, and siRNA applications were used to explore the biological consequence of CGN c.3560C > T.

Results

In cancer cell lines and orthotopic animal models, CGN c.3560C > T enhanced tumor progression with reduced sensitivity to oxaliplatin compared to the CGN WT. The variant induced downregulation of epithelial marker, upregulation of mesenchymal marker and transcription factor, which converged to initiate epithelial-mesenchymal transition (EMT). Proteomic analysis was conducted to investigate the elements driving EMT in CGN c.3560C > T. This exploration unveiled overexpression of IQGAP1 induced by the variant, contrasting the levels observed in CGN WT. Immunoprecipitation assay confirmed a direct interaction between CGN and IQGAP1. IQGAP1 functions as a regulator of multiple GTPases, particularly the Rho family. This overexpressed IQGAP1 was consistently associated with the activation of Rac1, as evidenced by the analysis of the cancer cell line and clinical sample harboring CGN c.3560C > T. Notably, activated Rac1 was suppressed following the downregulation of IQGAP1 by siRNA. Treatment with NSC23766, a selective inhibitor for Rac1-GEF interaction, resulted in the inactivation of Rac1. This intervention mitigated the EMT program in cancer cells carrying CGN c.3560C > T. Consistently, xenograft tumors with WT CGN showed no sensitivity to NSC23766 treatment, but NSC23766 demonstrated the capacity to attenuate tumor growth harboring c.3560C > T.

Conclusions

CGN c.3560C > T leads to IQGAP1 overexpression, subsequently triggering Rac1-dependent EMT. Targeting activated Rac1 is a strategy to impede the advancement of cancers carrying this specific variant.
GMP-manufactured CRISPR/Cas9 technology as an advantageous tool to support cancer immunotherapy
Authors: Caforio M , Iacovelli S , Quintarelli C , Locatelli F , Folgiero Valentina
Published: March 2024

Background

CRISPR/Cas9 system to treat human-related diseases has achieved significant results and, even if its potential application in cancer research is improving, the application of this approach in clinical practice is still a nascent technology.

Main body

CRISPR/Cas9 technology is not yet used as a single therapy to treat tumors but it can be combined with traditional treatment strategies to provide personalized gene therapy for patients. The combination with chemotherapy, radiation and immunotherapy has been proven to be a powerful means of screening, identifying, validating and correcting tumor targets. Recently, CRISPR/Cas9 technology and CAR T-cell therapies have been integrated to open novel opportunities for the production of more efficient CAR T-cells for all patients. GMP-compatible equipment and reagents are already available for several clinical-grade systems at present, creating the basis and framework for the accelerated development of novel treatment methods.

Conclusion

Here we will provide a comprehensive collection of the actual GMP-grade CRISPR/Cas9-mediated approaches used to support cancer therapy highlighting how this technology is opening new opportunities for treating tumors.
Field evaluation of an automated mosquito surveillance system which classifies Aedes and Culex mosquitoes by genus and sex
Authors: González-Pérez María I. , Faulhaber Bastian , Aranda Carles , Williams Mark , Villalonga Pancraç , Silva Manuel , Costa Osório Hugo , Encarnaçao Joao , Talavera Sandra , Busquets Núria
Published: March 2024

Background

Mosquito-borne diseases are a major concern for public and veterinary health authorities, highlighting the importance of effective vector surveillance and control programs. Traditional surveillance methods are labor-intensive and do not provide high temporal resolution, which may hinder a full assessment of the risk of mosquito-borne pathogen transmission. Emerging technologies for automated remote mosquito monitoring have the potential to address these limitations; however, few studies have tested the performance of such systems in the field.

Methods

In the present work, an optical sensor coupled to the entrance of a standard mosquito suction trap was used to record 14,067 mosquito flights of Aedes and Culex genera at four temperature regimes in the laboratory, and the resulting dataset was used to train a machine learning (ML) model. The trap, sensor, and ML model, which form the core of an automated mosquito surveillance system, were tested in the field for two classification purposes: to discriminate Aedes and Culex mosquitoes from other insects that enter the trap and to classify the target mosquitoes by genus and sex. The field performance of the system was assessed using balanced accuracy and regression metrics by comparing the classifications made by the system with those made by the manual inspection of the trap.

Results

The field system discriminated the target mosquitoes (Aedes and Culex genera) with a balanced accuracy of 95.5% and classified the genus and sex of those mosquitoes with a balanced accuracy of 88.8%. An analysis of the daily and seasonal temporal dynamics of Aedes and Culex mosquito populations was also performed using the time-stamped classifications from the system.

Conclusions

This study reports results for automated mosquito genus and sex classification using an optical sensor coupled to a mosquito trap in the field with highly balanced accuracy. The compatibility of the sensor with commercial mosquito traps enables the sensor to be integrated into conventional mosquito surveillance methods to provide accurate automatic monitoring with high temporal resolution of Aedes and Culex mosquitoes, two of the most concerning genera in terms of arbovirus transmission.

Graphical Abstract
Multi-omics data elucidate parasite-host-microbiota interactions and resistance to Haemonchus contortus in sheep
Authors: Niciura Simone Cristina Méo , Cardoso Tainã Figueiredo , Ibelli Adriana Mercia Guaratini , Okino Cintia Hiromi , Andrade Bruno Gabriel , Benavides Magda Vieira , Chagas Ana Carolina de Souza , Esteves Sergio Novita , Minho Alessandro Pelegrine , Regitano Luciana Correia de Almeida , Gondro Cedric
Published: March 2024

Background

The integration of molecular data from hosts, parasites, and microbiota can enhance our understanding of the complex biological interactions underlying the resistance of hosts to parasites. Haemonchus contortus, the predominant sheep gastrointestinal parasite species in the tropics, causes significant production and economic losses, which are further compounded by the diminishing efficiency of chemical control owing to anthelmintic resistance. Knowledge of how the host responds to infection and how the parasite, in combination with microbiota, modulates host immunity can guide selection decisions to breed animals with improved parasite resistance. This understanding will help refine management practices and advance the development of new therapeutics for long-term helminth control.

Methods

Eggs per gram (EPG) of feces were obtained from Morada Nova sheep subjected to two artificial infections with H. contortus and used as a proxy to select animals with high resistance or susceptibility for transcriptome sequencing (RNA-seq) of the abomasum and 50 K single-nucleotide genotyping. Additionally, RNA-seq data for H. contortus were generated, and amplicon sequence variants (ASV) were obtained using polymerase chain reaction amplification and sequencing of bacterial and archaeal 16S ribosomal RNA genes from sheep feces and rumen content.

Results

The heritability estimate for EPG was 0.12. GAST, GNLY, IL13, MGRN1, FGF14, and RORC genes and transcripts were differentially expressed between resistant and susceptible animals. A genome-wide association study identified regions on chromosomes 2 and 11 that harbor candidate genes for resistance, immune response, body weight, and adaptation. Trans-expression quantitative trait loci were found between significant variants and differentially expressed transcripts. Functional co-expression modules based on sheep genes and ASVs correlated with resistance to H. contortus, showing enrichment in pathways of response to bacteria, immune and inflammatory responses, and hub features of the Christensenellaceae, Bacteroides, and Methanobrevibacter genera; Prevotellaceae family; and Verrucomicrobiota phylum. In H. contortus, some mitochondrial, collagen-, and cuticle-related genes were expressed only in parasites isolated from susceptible sheep.

Conclusions

The present study identified chromosome regions, genes, transcripts, and pathways involved in the elaborate interactions between the sheep host, its gastrointestinal microbiota, and the H. contortus parasite. These findings will assist in the development of animal selection strategies for parasite resistance and interdisciplinary approaches to control H. contortus infection in sheep.

Graphical Abstract
Intrafamily heterooligomerization as an emerging mechanism of methyltransferase regulation
Authors: Hobble Haley V. , Schaner Tooley Christine E.
Published: March 2024

Protein and nucleic acid methylation are important biochemical modifications. In addition to their well-established roles in gene regulation, they also regulate cell signaling, metabolism, and translation. Despite this high biological relevance, little is known about the general regulation of methyltransferase function. Methyltransferases are divided into superfamilies based on structural similarities and further classified into smaller families based on sequence/domain/target similarity. While members within superfamilies differ in substrate specificity, their structurally similar active sites indicate a potential for shared modes of regulation. Growing evidence from one superfamily suggests a common regulatory mode may be through heterooligomerization with other family members. Here, we describe examples of methyltransferase regulation through intrafamily heterooligomerization and discuss how this can be exploited for therapeutic use.
Dynamic whole-body [18F]FES PET/CT increases lesion visibility in patients with metastatic breast cancer
Authors: Pedersen Mette A. , Munk Ole L. , Dias André H. , Steffensen Johanne H. , Møller Anders L. , Johnsson Anna Lyhne , Hansen Kim Vang , Bender Dirk , Jakobsen Steen , Busk Morten , Gormsen Lars C. , Tramm Trine , Borgquist Signe , Vendelbo Mikkel H.
Published: March 2024

Background

Correct classification of estrogen receptor (ER) status is essential for prognosis and treatment planning in patients with breast cancer (BC). Therefore, it is recommended to sample tumor tissue from an accessible metastasis. However, ER expression can show intra- and intertumoral heterogeneity. 16α-[18F]fluoroestradiol ([18F]FES) Positron Emission Tomography/Computed Tomography (PET/CT) allows noninvasive whole-body (WB) identification of ER distribution and is usually performed as a single static image 60 min after radiotracer injection. Using dynamic whole-body (D-WB) PET imaging, we examine [18F]FES kinetics and explore whether Patlak parametric images ($${K}_{i}$$

K
i

) are quantitative and improve lesion visibility.

Results

This prospective study included eight patients with metastatic ER-positive BC scanned using a D-WB PET acquisition protocol. The kinetics of [18F]FES were best characterized by the irreversible two-tissue compartment model in tumor lesions and in the majority of organ tissues. $${K}_{i}$$

K
i

values from Patlak parametric images correlated with $${K}_{i}$$

K
i

values from the full kinetic analysis, r2 = 0.77, and with the semiquantitative mean standardized uptake value (SUVmean), r2 = 0.91. Furthermore, parametric $${K}_{i}$$

K
i

images had the highest target-to-background ratio (TBR) in 162/164 metastatic lesions and the highest contrast-to-noise ratio (CNR) in 99/164 lesions compared to conventional SUV images. TBR was 2.45 (95% confidence interval (CI): 2.25–2.68) and CNR 1.17 (95% CI: 1.08–1.26) times higher in $${K}_{i}$$

K
i

images compared to SUV images. These quantitative differences were seen as reduced background activity in the $${K}_{i}$$

K
i

images.

Conclusion

[18F]FES uptake is best described by an irreversible two-tissue compartment model. D-WB [18F]FES PET/CT scans can be used for direct reconstruction of parametric $${K}_{i}$$

K
i

images, with superior lesion visibility and $${K}_{i}$$

K
i

values comparable to $${K}_{i}$$

K
i

values found from full kinetic analyses. This may aid correct ER classification and treatment decisions.

Trial registration ClinicalTrials.gov: NCT04150731, https://clinicaltrials.gov/study/NCT04150731
Contribution of environmental determinants to the risk of developing type 2 diabetes mellitus in a life-course perspective: a systematic review protocol
Authors: Mengue Yannick Wilfried , Audate Pierre-Paul , Dubé Jean , Lebel Alexandre
Published: March 2024

Background

Prevention policies against type 2 diabetes mellitus (T2DM) focus solely on individual healthy lifestyle behaviours, while an increasing body of research recognises the involvement of environmental determinants (ED) (cultural norms of land management and planning, local foodscape, built environment, pollution, and neighbourhood deprivation). Precise knowledge of this relationship is essential to proposing a prevention strategy integrating public health and spatial planning. Unfortunately, issues related to the consistency and synthesis of methods, and results in this field of research limit the development of preventive strategies. This systematic review aims to improve knowledge about the relationship between the risk of developing T2DM in adulthood and long-term exposure to its ED during childhood or teenage years.

Methods

This protocol is presented according to the Preferred Reporting Items for Systematic Review and Meta-Analysis Protocols (PRISMA-P) tools. PubMed, Embase, CINAHL, Web of Science, EBSCO, and grey literature from the Laval University Libraries databases will be used for data collection on main concepts such as ‘type 2 diabetes mellitus’, ‘zoning’ or ‘regional, urban, or rural areas land uses’, ‘local food landscape’, ‘built environment’, ‘pollution’, and ‘deprivation’. The Covidence application will store the collected data for selection and extraction based on the Population Exposure Comparator Outcome and Study design approach (PECOS). Studies published until December 31, 2023, in English or French, used quantitative data about individuals aged 18 and over that report on T2DM, ED (cultural norms of land management and planning, local foodscape, built environment, and neighbourhood deprivation), and their association (involving only risk estimators) will be included. Then, study quality and risk of bias will be conducted according to the combined criteria and ratings from the ROBINS-E (Risk of Bias in Non-randomised Studies—of Exposures) tools and the ‘Effective Public Health Practice Project’ (EPHPP). Finally, the analytical synthesis will be produced using the ‘Synthesis Without Meta-analysis’ (SWiM) guidelines.

Discussion

This systematic review will summarise available evidence on ED associated with T2DM. The results will contribute to improving current knowledge and developing more efficient cross-sectoral interventions in land management and public health in this field of research.

Systematic review registration

PROSPERO CRD42023392073.
The SAFE procedure: a practical stopping heuristic for active learning-based screening in systematic reviews and meta-analyses
Authors: Boetje Josien , van de Schoot Rens
Published: March 2024

Active learning has become an increasingly popular method for screening large amounts of data in systematic reviews and meta-analyses. The active learning process continually improves its predictions on the remaining unlabeled records, with the goal of identifying all relevant records as early as possible. However, determining the optimal point at which to stop the active learning process is a challenge. The cost of additional labeling of records by the reviewer must be balanced against the cost of erroneous exclusions. This paper introduces the SAFE procedure, a practical and conservative set of stopping heuristics that offers a clear guideline for determining when to end the active learning process in screening software like ASReview. The eclectic mix of stopping heuristics helps to minimize the risk of missing relevant papers in the screening process. The proposed stopping heuristic balances the costs of continued screening with the risk of missing relevant records, providing a practical solution for reviewers to make informed decisions on when to stop screening. Although active learning can significantly enhance the quality and efficiency of screening, this method may be more applicable to certain types of datasets and problems. Ultimately, the decision to stop the active learning process depends on careful consideration of the trade-off between the costs of additional record labeling against the potential errors of the current model for the specific dataset and context.
Water quality, habitat, and fish assemblage relationships in middle-order agriculture and forest streams of the Mississippi Alluvial Plain
Authors: Skoog Matthew L. , Eggleton Michael A. , Chen Yushun
Published: March 2024

Background

Agriculture has greatly influenced water quality, habitats, and fish assemblages in streams of the Mississippi Alluvial Plain (MAP) ecoregion. However, MAP streams have historically been understudied compared to streams in other agricultural regions of the USA. In this study, water quality, habitat, and fish assemblage composition were assessed seasonally (spring, summer, and fall) in eight representative MAP streams located across three U.S. states. The study design included four streams containing highly agricultural watersheds (herein termed “agriculture” streams) and four streams containing mostly forested watersheds (herein termed “forest” streams), which were intended to represent reference conditions for MAP streams.

Results

In general, forest streams contained significantly better instream and riparian habitats than agriculture streams (P = 0.010–0.040) whereas agriculture streams contained significantly greater levels of primary nutrients (P < 0.001–0.010). Differences between agriculture and forest streams with respect to other physical and chemical variables were intermittent and season dependent. Fish assemblages in agriculture and forest streams were structured primarily along an environmental gradient reflecting instream habitat conditions, water nutrient concentrations, and benthic chlorophyll-a production. Structurally, fish assemblages in both stream types contained many regionally common species, though some species appeared to exhibit affinities for a particular stream type. Functionally, fish assemblages in agriculture streams contained more tolerant species, more omnivores, and fewer insectivores compared to forest stream assemblages, which were nearly all insectivores. Overall, one-third of the fish specimens collected in forest streams classified as intolerant species.

Conclusions

Our results suggested that stream water quality, habitat, and fish assemblages differed between agriculture and forest streams in the MAP, with fish assemblages exhibiting both structural and functional differences. Results were consistent with a larger body of literature from smaller, headwater streams whereby land-use changes (e.g., row-crop agriculture) impacted the physical, chemical, and biological characteristics of stream ecosystems. Results further highlight the importance of land use management and its effects on habitat diversity in stream ecosystems, and that protecting the few remaining undisturbed or less-disturbed streams should be a priority.
Clinical evidence of the link between gut microbiome and myalgic encephalomyelitis/chronic fatigue syndrome: a retrospective review
Authors: Wang Jing-Hua , Choi Yujin , Lee Jin-Seok , Hwang Seung-Ju , Gu Jiyeon , Son Chang-Gue
Published: March 2024

Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) is a heterogeneous disorder with elusive causes, but most likely because of clinical and other biological factors. As a vital environmental factor, the gut microbiome is increasingly emphasized in various refractory diseases including ME/CFS. The present study is aimed to enhance our understanding of the relationship between the gut microbiome and ME/CFS through data analysis of various clinical studies. We conducted a literature search in four databases (PubMed, Cochrane Library, Web of Science, and Google Scholar) until May 31, 2023. Our analysis encompassed 11 clinical studies with 553 ME/CFS patients and 480 healthy controls. A comparative analysis of meta data revealed a significant decrease in α-diversity and a noticeable change in β-diversity in the gut microbiome of ME/CFS patients compared to healthy controls. The notable ratio of Firmicutes and Bacteroides was 2.3 times decreased, and also, there was a significant reduction in the production of microbial metabolites such as acetate, butyrate, isobutyrate, and some amino acids (alanine, serine, and hypoxanthine) observed in ME/CFS patients. The lack of comparison under similar conditions with various standardized analytical methods has impeded the optimal calculation of results in ME/CFS patients and healthy controls. This review provides a comprehensive overview of the recent advancements in understanding the role of the gut microbiome in ME/CFS patients. Additionally, we have also discussed the potentials of using microbiome-related interventions and associated challenges to alleviate ME/CFS.
Residual calcified material volume of β–tricalcium phosphate with platelet-rich fibrin in unilateral alveolar bone graft
Authors: Ho Nguyen Chon T. , Bui Minh H. , Lam Phuong H.
Published: March 2024

Background

This study aimed to evaluate the effectiveness of β-tricalcium phosphate (β-TCP) and platelet-rich fibrin (PRF) in unilateral alveolar bone graft, involving the percentage of residual calcified material and the average labiopalatal thickness of the grafts on cone beam computed tomography at 6 months after surgery, comparing two age groups 12 years and under and over 12 years old.

Results

The mean preoperative defect volume was 0.93 ± 0.20 cm3, with no significant difference between the two groups (p = 0.652). In the postoperative period, we did not record any abnormal bleeding and no infection was observed. Six months after surgery, the mean percentage of residual calcified material was 63.53 ± 16.48% with a significantly higher difference in the age group 12 and under (p < 0.001), and the mean average labiopalatal thickness of the grafted bone was 5.72 ± 1.09 mm with a significantly higher difference in the age group 12 and under (p = 0.011).

Conclusion

Using β-TCP and PRF in alveolar bone graft surgery has acceptable effectiveness clinically and on CBCT images, with significantly higher differences of the percentage of residual calcified material and the average labiopalatal thickness of the grafted bone in the group 12 years old and younger than in the older group.
Audiologists’ practice and perspectives on rehabilitation in individuals with minimal-mild degree of hearing loss: a questionnaire-based survey
Authors: Goyal Swati , E. Prajwal Kumar , Dutta Kristi Kaveri , S. Gowtham Varma , Thomas Prajeesh
Published: March 2024

Background

The study aimed to understand the perspectives of audiologists working in different audiological setups regarding the fitting of amplification devices for individuals with minimal-mild hearing loss which cannot be corrected or sensorineural in nature. The study employed a questionnaire-based survey research design conducted in three phases: questionnaire development, obtaining responses from audiologists, and analyzing the responses. The questionnaire consisted of three sections: demographic details, audiologist's practice, and audiologist's opinion, with a total of 22 questions. The responses from audiologists were collected through an online survey distributed via WhatsApp groups, email, and Telegram. Descriptive statistics were used to analyze the percentage of responses for each question.

Results

The findings shed light on the current practices and opinions of audiologists. The study found that audiologists perceived the assessment of self-handicap level as aiding in better counseling, understanding motivation level, and making decisions about amplification.

Conclusions

The findings of the study provide insights into the current practices and perspectives of audiologists in offering services to individuals with minimal-mild hearing loss.
Two-staged hybrid repair of multiple great vessel and thoracic aneurysms with right vocal fold palsy using retrograde in situ branched stent grafting: a case report
Authors: Imamura Yuki , Saito Yoshiaki , Murata Kenyu , Itokawa Rin , Taguchi Ryo , Minakawa Masahito
Published: March 2024

Background

Repair of multiple thoracic aneurysms with vocal fold palsy represents a surgical challenge. Here, we present a case of multiple cervical and thoracic aortic aneurysms with right vocal fold palsy successfully treated with two-stage hybrid repair using retrograde in situ branched grafting.

Case presentation

A 52-year-old patient with multiple cervical and thoracic aortic aneurysms and right vocal fold palsy was treated using two-stage hybrid repair. Initially, the patient underwent an open partial arch replacement with reconstruction of the right subclavian, right common carotid, and left common carotid arteries. Three months after the initial procedure, the left subclavian artery was reconstructed during thoracic endovascular aortic repair with retrograde in situ stent grafting using the needle technique. The postoperative course was uneventful with no endoleaks detected using postoperative computed tomography.

Conclusion

This case highlighted the potential of two-stage repair of multiple thoracic aneurysms using retrograde in situ stent grafting. With the expansion of surgical options to include endovascular and hybrid approaches, surgical procedures should be carefully planned for individuals with complex aortic diseases.
Fault locking and alternate fault activity revealed by simple tools and approaches — Examples from the Western Canadian Sedimentary Basin with implications for hydrocarbon exploration and production
Authors: Chatellier Jean-Yves
Published: March 2024

The geology toolkit that is used to reveal faults and fractures is much wider than before. This is due to 3D and 4D views in exploratory statistics programs and to the availability of user-friendly GIS software. These tools allow us to visualize a multitude of parameters that will be briefly explored here. A review of many geologic and nongeologic parameters led to evidence of fault locking and alternate fault activity. It also resulted in new structural models for the Western Canadian Sedimentary Basin (WCSB). The presented data sets include earthquakes, drilling, production, well data, aeromagnetic data, and more. Various integrated approaches reveal well-defined fault patterns that are typical of a strike-slip regime and the existence of previously unrecognized detachments that are important for hydrocarbon exploration. Some of the new geometries and associated mechanisms are illustrated here with outcrop analogues and present-day cross sections, maps, and 3D views. Only the most recent of the two identified strike-slip regimes is covered in this paper. Some emphasis is given to the recognition of detachments at various scales. Among these is the importance of megadetachments displacing the sedimentary cover by up to 16 km with respect to the aeromag. Hence, there is a need for reconstruction before making conclusions. The WCSB has a lot more to offer to explorers who understand faults, fractures, and migration paths. Integrating many types of information in map or 3D views offers new tools to identify and characterize faults.
Delineating subbasins of sedimentary rock structure beneath eastern Central Sumatra Basin based on gravity model, seismic profiles, and well-log data: A case study
Authors: Nainggolan Tumpal Bernhard , Kusnida Dida , Mirnanda Eddy , Setiadi Imam , Nugroho Erwin Haryanto , Subagio
Published: March 2024

The Central Sumatra Basin is a vast sedimentary basin that has been proven to produce hydrocarbon. The basin comprises several subbasins that are not sufficiently imaged by conventional seismic reflection profiles and limited well-log data, particularly in the nearshore area to the east. This research aims to delineate sedimentary subbasins, interpret the subsurface geologic model, and identify geologic structures beneath the eastern part of the Central Sumatra Basin using integrated geophysical gravity, seismic profiles, and well-log data. Three-dimensional gravity inversion modeling results indicate that the pre-Tertiary granitic basement is a continental crust with a mass density value of 2.67 gr/cc. The modeling results indicate that the sedimentary rock is composed of Early Oligocene–Middle Miocene sedimentary rock, with a mass density of 2.35 gr/cc, arranged from bottom to top. The residual gravity anomaly model identifies 13 sedimentary subbasins with structural features such as basement height, graben, and fault mapped in a relatively northwest–southeast direction. Moreover, based on the graben pattern and the basement high beneath the eastern Central Sumatra Basin, many structural patterns support the development of petroleum systems similar to that of the western part of the basin, which has already produced hydrocarbon. Our research also revealed the thickness of the Sihapas Formation in the eastern part of the basin, which shows great potential as a hydrocarbon reservoir. The results show that integrated analysis of many geophysical data sets can substantially decrease the uncertainty associated with individual data sets and produce more reliable imaging of subsurface geology.
Baseline symptom severity and efficacy of Silexan in patients with anxiety disorders: A symptom-based, patient-level analysis of randomized, placebo-controlled trials
Authors: Dold Markus , Möller Hans-Jürgen , Volz Hans-Peter , Seifritz Erich , Schläfke Sandra , Bartova Lucie , Kasper Siegfried
Published: March 2024

The influence of baseline severity on the efficacy of Silexan, a proprietary essential oil from Lavandula angustifolia, in anxiety disorders has not been investigated in a pooled dataset. We report on an individual patient data analysis of all five double-blind, randomized, placebo-controlled trials with Silexan in anxiety disorders. Eligible participants received Silexan 80 mg/d or placebo for 10 weeks. Analyses were based on the Hamilton Anxiety Rating Scale (HAMA), its psychic and somatic anxiety subscores, and the Clinical Global Impressions (CGI) scale. To correlate baseline severity with outcome, patients were segregated into mild, moderate, and severe cases. Altogether 1,172 patients (Silexan, n = 587; placebo, n = 585) were analyzed. For the HAMA total score, we found a significant association between the score at baseline and the treatment effect of Silexan versus placebo at week 10 (p < 0.001). HAMA items from the somatic domain scored lower at baseline and showed less improvement than items from the psychic domain, particularly in patients with mild or moderate baseline symptoms. For CGI item 2 (global improvement), significant efficacy favoring Silexan were observed in mild, moderate, and severe baseline symptom severity. Although significant improvements were found for all subsets, the more severe the initial symptoms, the greater the treatment effects documented by the HAMA. Overall this analysis confirms that Silexan is an effective treatment option in early or mild stages of anxiety disorder. Given its favorable safety profile, Silexan can thus fill a therapeutic gap in the treatment of (subsyndromal) anxiety disorders.