bluuebunny/crossref_metadata_2025 huggingface dataset:
Exopolyhedral Ligand Orientation Controls Diastereoisomer in Mixed-Metal Bis(Carboranes)
Authors: Chan Antony P. Y. , Rosair Georgina M. , Welch Alan J.
Published: January 2020

Heterobimetallic derivatives of a bis(carborane), [μ7,8-(1′,3′−3′-Cl-3′-PPh3-closo-3′,1′,2′-RhC2B9H10)-2-(p-cymene)-closo-2,1,8-RuC2B9H10] (1) and [μ7,8-(1′,3′−3′-Cl-3′-PPh3-closo-3′,1′,2′-RhC2B9H10)-2-Cp-closo-2,1,8-CoC2B9H10] (2) have been synthesised and characterised, including crystallographic studies. A minor co-product during the synthesis of compound 2 is the new species [8-{8′-2′-H-2′,2′-(PPh3)2-closo-2′,1′,8′-RhC2B9H10}-2-Cp-closo-2,1,8-CoC2B9H10] (3), isolated as a mixture of diastereoisomers. Although, in principle, compounds 1 and 2 could also exist as two diastereoisomers, only one (the same in both cases) is formed. It is suggested that the preferred exopolyhedral ligand orientation in the rhodacarboranes in the non-observed diastereoisomers would lead to unacceptable steric crowding between the PPh3 ligand and either the p-cymene (compound 1) or Cp (compound 2) ligand of the ruthenacarborane or cobaltacarborane, respectively.
Fast Isolation of Flavonoids from the Endemic Species Nolana ramosissima I.M. Johnst and Its Endothelium-Independent Relaxation Effect in Rat Aorta
Authors: Cifuentes Fredi , Palacios Javier , Bórquez Jorge , Paredes Adrián , Parra Claudio , Bravo Alejandra , Simirgiotis Mario J.
Published: January 2020

The infusion of the desertic plant Nolana ramosissima I.M. Johnst showed vascular smooth muscle relaxation in rat aorta and the presence of several phenolic compounds, which were detected by high resolution UHPLC-Orbitrap-HESI-MS. In addition, five flavonoids were rapidly isolated from a methanolic extract using high-performance counter-current chromatography (HPCCC). The N. ramosissima extract showed endothelium-independent relaxation effect in rat aorta. Sixty-one compounds were detected in the infusion, mainly glycosylated flavonoids, flavanones and several oxylipins, suggesting that a synergistic effect between the compounds in the extracts could be responsible for the relaxation activity. Vascular activity experiments were done in isolated organ bath. In rat aorta, a nitric oxide inhibitor did not prevent the relaxation effects of the extract; however, a selective guanylyl cyclase inhibitor partially blunted this effect. The compound 5,3′-dihydroxy-4′7-dimethoxyflavone presented higher relaxation effect than 100 μg/mL of N. ramosissima extract. The extract and the isolated metabolites from N. ramosissima can show relaxation effects on rat aorta by a mechanism that is independent of the endothelium.
Alkoxy- and Silanol-Functionalized Cage-Type Oligosiloxanes as Molecular Building Blocks to Construct Nanoporous Materials
Authors: Shimojima Atsushi , Kuroda Kazuyuki
Published: January 2020

Siloxane-based materials have a wide range of applications. Cage-type oligosiloxanes have attracted significant attention as molecular building blocks to construct novel siloxane-based nanoporous materials with promising applications such as in catalysis and adsorption. This paper reviews recent progress in the preparation of siloxane-based nanoporous materials using alkoxy- and silanol-functionalized cage siloxanes. The arrangement of cage siloxanes units is controlled by various methods, including amphiphilic self-assembly, hydrogen bonding of silanol groups, and regioselective functionalization, toward the preparation of ordered nanoporous siloxane-based materials.
Substituted Piperazines as Novel Potential Radioprotective Agents
Authors: Filipova Alzbeta , Marek Jan , Havelek Radim , Pejchal Jaroslav , Jelicova Marcela , Cizkova Jana , Majorosova Martina , Muckova Lubica , Kucera Tomas , Prchal Lukas , Psotka Miroslav , Zivna Natalie , Koutova Darja , Sinkorova Zuzana , Rezacova Martina , Tichy Ales
Published: January 2020

The increasing risk of radiation exposure underlines the need for novel radioprotective agents. Hence, a series of novel 1-(2-hydroxyethyl)piperazine derivatives were designed and synthesized. Some of the compounds protected human cells against radiation-induced apoptosis and exhibited low cytotoxicity. Compared to the previous series of piperazine derivatives, compound 8 exhibited a radioprotective effect on cell survival in vitro and low toxicity in vivo. It also enhanced the survival of mice 30 days after whole-body irradiation (although this increase was not statistically significant). Taken together, our in vitro and in vivo data indicate that some of our compounds are valuable for further research as potential radioprotectors.
TBAB-Catalyzed 1,6-Conjugate Sulfonylation of para-Quinone Methides: A Highly Efficient Approach to Unsymmetrical gem-Diarylmethyl Sulfones in Water
Authors: Liu Zhang-Qin , You Peng-Sheng , Zhang Liang-Dong , Liu Da-Qing , Liu Sheng-Shu , Guan Xiao-Yu
Published: January 2020

A highly efficient sulfonylation of para-quinone methides with sulfonyl hydrazines in water has been developed on the basis of the mode involving a tetrabutyl ammonium bromide (TBAB)-promoted sulfa-1,6-conjugated addition pathway. This reaction provides a green and sustainable method to synthesize various unsymmetrical diarylmethyl sulfones, showing good functional group tolerance, scalability, and regioselectivity. Further transformation of the resulting diarylmethyl sulfones provides an efficient route to some functionalized molecules.
Novel Cationic Prodrug of Ubiquinol-10 Enhances Intestinal Absorption via Efficient Formation of Nanosized Mixed-Micelles with Bile Acid Anions
Authors: Setoguchi Shuichi , Hidaka Ryoji , Nagata-Akaho Nami , Watase Daisuke , Koga Mitsuhisa , Matsunaga Kazuhisa , Karube Yoshiharu , Takata Jiro
Published: January 2020

The aim of this study was to develop a prodrug of ubiquinol-10 (UqH-10), the active form of ubiquinone-10 (Uq-10), for oral delivery. Bioavailability of UqH-10 is hampered by its high susceptibility to oxidation and water-insolubility. We prepared three novel N,N-dimethylglycine ester derivatives of UqH-10, including a 1-monoester (UqH-1-DMG), 4-monoester (UqH-4-DMG), and 1,4-bis-ester (UqH-DMG), and assessed their physicochemical properties in vitro and in vivo. UqH-DMG spontaneously formed an aqueous micelle solution comprising 20 nm particles at 36.5 °C. Cationic UqH-DMG formed nano-sized (5 nm) mixed-micelles with taurocholic acid. Reconversion of the derivatives to UqH-10 was accelerated in human liver microsomes. The oral bioavailability of UqH-10 after administration of UqH-derivatives or Uq-10 was determined in fasted and postprandial rats secreting normal and high levels of bile, respectively. In fasted rats, plasma UqH-10 after UqH-derivatives administration reached Cmax at 2–3 h and after Uq-10 administration, it remained low. The AUC0-24h of UqH-10 after UqH-derivatives administration was 2–3-fold higher than that after Uq-10 administration. In postprandial rats, the Tmax of UqH-10 after UqH-derivatives administration was an hour earlier than after Uq-10 administration. In conclusion, cationic UqH-derivatives are convenient prodrugs that enhance UqH-10 bioavailability by forming nanosized mixed-micelles with intestinal bile acids.
Thermal Stability of Octadecyltrichlorosilane and Perfluorooctyltriethoxysilane Monolayers on SiO2
Authors: Yang Xiangdong , Wang Haitao , Wang Peng , Yang Xuxin , Mao Hongying
Published: January 2020

Using in situ ultraviolet photoelectron spectroscopy (UPS) and X-ray photoelectron spectroscopy (XPS) measurements, the thermal behavior of octadecyltrichlorosilane (OTS) and 1H, 1H, 2H, and 2H-perfluorooctyltriethoxysilane (PTES) monolayers on SiO2 substrates has been investigated. OTS is thermally stable up to 573 K with vacuum annealing, whereas PTES starts decomposing at a moderate temperature between 373 K and 423 K. Vacuum annealing results in the decomposition of CF3 and CF2 species rather than desorption of the entire PTES molecule. In addition, our UPS results reveal that the work function (WF)of OTS remains the same after annealing; however WF of PTES decreases from ~5.62 eV to ~5.16 eV after annealing at 573 K.
Facile Synthesis and Reuse of Magnetic Black Carbon Magnetite (BC-Mag) for Fast Carbamazepine Removal from Water
Authors: Cai Nan , Larese-Casanova Philip
Published: January 2020

Magnetic carbonaceous nanomaterials are needed in water treatment applications because they can offer both carbon surfaces for sorption of organic pollutants and ease of material magnetic retrieval for regeneration and reuse. In this study, we employed a facile one-step method to synthesize a black carbon-magnetite composite (BC-Mag) by high-temperature annealing of black carbon and hematite. The nanocomposite was easily dispersed and stable in water owing to the presence of negatively charged oxygen surface functional groups. Sorption kinetics with dissolved carbamazepine showed a rapid initial uptake with equilibrium achieved within only minutes. The sorption extent can be described with the Freundlich model, and surface area normalized sorption affinity was an order of magnitude greater than conventional granular activated carbon. The sorption extent of neutral carbamazepine remained constant between pH 2–10 while surface zeta potential decreased. BC-Mag can be reused for the sorption of carbamazepine up to six times without significant loss of the sorption extent.
Repeated vs. Acute Exposure of RAW264.7 Mouse Macrophages to Silica Nanoparticles: A Bioaccumulation and Functional Change Study
Authors: Torres Anaëlle , Dalzon Bastien , Collin-Faure Véronique , Rabilloud Thierry
Published: January 2020

Synthetic amorphous silica is used in various applications such as cosmetics, food, or rubber reinforcement. These broad uses increase human exposure, and thus the potential risk related to their short- and long-term toxicity for both consumers and workers. These potential risks have to be investigated, in a global context of multi-exposure, as encountered in human populations. However, most of the in vitro research on the effects of amorphous silica has been carried out in an acute exposure mode, which is not the most relevant when trying to assess the effects of occupational exposure. As a first step, the effects of repeated exposure of macrophages to silica nanomaterials have been investigated. The experiments have been conducted on in vitro macrophage cell line RAW264.7 (cell line from an Abelson murine leukemia virus-induced tumor), as this cell type is an important target cell in toxicology of particulate materials. The bioaccumulation of nanomaterials and the persistence of their effects have been studied. The experiments carried out include the viability assay and functional tests (phagocytosis, NO and reactive oxygen species dosages, and production of pro- and anti-inflammatory cytokines) using flow cytometry, microscopy and spectrophotometry. Accumulation of silica nanoparticles (SiO2 NP) was observed in both exposure scenarii. However, differences in the biological effects between the exposure scenarii have also been observed. For phagocytosis, NO production and Tumor Necrosis Factor (TNF) release, repeated exposure tended to induce fewer effects than acute exposure. Nevertheless, repeated exposure still induces alterations in the macrophage responses and thus represents a scenario to be tested in detail.
Directly Electrospun Carbon Nanofibers Incorporated with Mn3O4 Nanoparticles as Bending-Resistant Cathode for Flexible Al-Air Batteries
Authors: Yu Ying , Zuo Yuxin , Liu Ying , Wu Youjun , Zhang Zhonghao , Cao Qianqian , Zuo Chuncheng
Published: January 2020

Al-air batteries are regarded as potential power source for flexible and wearable devices. However, the traditional cathodes of Al-air batteries are easy to be broken after continuous bending. This is why few Al-air batteries have been tested under the state of dynamic bending so far. Herein, carbon nanofibers incorporated with Mn3O4 catalyst have been prepared as bending-resistant cathodes through direct electrospinning. The cathode assembled in Al-air battery showed excellent electrochemical and mechanical stability. A high specific capacity of 1021 mAh/cm2 was achieved after bending 1000 times, which is 81.7% of that in platform state. This work will facilitate the progress of using Al-air battery in flexible electronics.
Safety Evaluations of Single Dose of the Olive Secoiridoid S-(−)-Oleocanthal in Swiss Albino Mice
Authors: Siddique Abu Bakar , King Judy Ann , Meyer Sharon A. , Abdelwahed Khaldoun , Busnena Belnaser , El Sayed Khalid A.
Published: January 2020

Epidemiological and clinical studies compellingly showed the ability of Mediterranean diet rich in extra-virgin olive oil (EVOO) to reduce multiple diseases such as cancer, cardiovascular diseases, and aging cognitive functions decline. The S-(−)-Oleocanthal (OC) is a minor phenolic secoiridoid exclusively found in extra-virgin olive oil (EVOO). OC recently gained notable research attention due to its excellent in vitro and in vivo biological effects against multiple cancers, inflammations, and Alzheimer’s disease. However, OC safety has not been comprehensively studied yet. This study reports for the first time the detailed safety of oral single OC dose in Swiss albino mice, applying the OECD 420 procedure. Male and female Swiss albino mice (n = 10) were orally treated with a single OC dose of either 10, 250, or 500 mg/kg bodyweight or equivalent volumes of distilled water. Mice fed a regular diet, and carefully observed for 14 days. Further, mice were then sacrificed, blood samples, and organs were collected and subjected to hematological, biochemical, and histological examinations. OC 10 mg/kg oral dose appears to be without adverse effects. Further, 250 mg/kg OC, p.o., is suggested as a possible upper dose for preclinical studies in the future.
Associations of Breast Milk Microbiota, Immune Factors, and Fatty Acids in the Rat Mother–Offspring Pair
Authors: Azagra-Boronat Ignasi , Tres Alba , Massot-Cladera Malén , Franch Àngels , Castell Margarida , Guardiola Francesc , Pérez-Cano Francisco J. , Rodríguez-Lagunas Maria J.
Published: January 2020

The present study aimed to analyze the rat breast milk profile of fatty acids (FA), immunoglobulins (Ig), microbiota, and their relationship, and to further assess their associations in the mother–offspring pair. Dams were monitored during the three weeks of gestation, allowed to deliver at term, and followed during two weeks of lactation. At the end of the study, milk was obtained from the dams for the analysis of fatty acids, microbiota composition, immunoglobulins, and cytokines. Moreover, the cecal content and plasma were obtained from both the dams and pups to study the cecal microbiota composition and the plasmatic levels of fatty acids, immunoglobulins, and cytokines. Rat breast milk lipid composition was ~65% saturated FA, ~15% monounsaturated FA, and ~20% polyunsaturated FA. Moreover, the proportions of IgM, IgG, and IgA were ~2%, ~88%, and ~10%, respectively. Breast milk was dominated by members of Proteobacteria, Firmicutes, and Bacteroidetes phyla. In addition, forty genera were shared between the milk and cecal content of dams and pups. The correlations performed between variables showed, for example, that all IgGs subtypes correlated between the three compartments, evidencing their association in the mother-milk-pup line. We established the profile of FA, Ig, and the microbiota composition of rat breast milk. Several correlations in these variables evidenced their association through the mother-milk-pup line. Therefore, it would be interesting to perform dietary interventions during pregnancy and/or lactation that influence the quality of breast milk and have an impact on the offspring.
A Fermented Milk Product with B. lactis CNCM I-2494 and Lactic Acid Bacteria Improves Gastrointestinal Comfort in Response to a Challenge Diet Rich in Fermentable Residues in Healthy Subjects
Authors: Le Nevé Boris , Martinez-De la Torre Adrian , Tap Julien , Derrien Muriel , Cotillard Aurélie , Barba Elizabeth , Mego Marianela , Nieto Ruiz Adoración , Hernandez-Palet Laura , Dornic Quentin , Faurie Jean-Michel , Butler John , Merino Xavi , Lobo Beatriz , Pinsach Batet Ferran , Accarino Anna , Pozuelo Marta , Manichanh Chaysavanh , Azpiroz Fernando
Published: January 2020

Background: Healthy plant-based diets rich in fermentable residues may induce gas-related symptoms. Our aim was to determine the potential of a fermented milk product with probiotics in improving digestive comfort with such diets. Methods: In an open design, a 3-day high-residue diet was administered to healthy subjects (n = 74 included, n = 63 completed) before and following 28 days consumption of a fermented milk product (FMP) containing Bifidobacterium animalis subsp. lactis CNCM I-2494 and lactic acid bacteria. Main outcomes: digestive sensations, number of daytime anal gas evacuations, and gas volume evacuated during 4 h after a probe meal. Results: As compared to the habitual diet, the high-residue diet induced gas-related symptoms (flatulence score 4.9 vs. 1.2; p ≤ 0.0001), increased the daily number of anal gas evacuations (20.7 vs. 8.7; p < 0.0001), and impaired digestive well-being (1.0 vs. 3.4; p < 0.05). FMP consumption reduced flatulence sensation (by −1.7 [−1.9; −1.6]; p < 0.0001), reduced the number of daily evacuations (by −5.8 [−6.5; −5.1]; p < 0.0001), and improved digestive well-being (by +0.6 [+0.4; +0.7]; p < 0.05). FMP consumption did not affect the gas volume evacuated after a probe meal. Conclusion: In healthy subjects, consumption of a FMP containing B. lactis CNCM I-2494 and lactic acid bacteria improves the tolerance of a flatulogenic diet by subjective and objective criteria (sensations and number of anal gas evacuations, respectively).
The Effects of a 6-Month High Dose Omega-3 and Omega-6 Polyunsaturated Fatty Acids and Antioxidant Vitamins Supplementation on Cognitive Function and Functional Capacity in Older Adults with Mild Cognitive Impairment
Authors: S. Stavrinou Pinelopi , Andreou Eleni , Aphamis George , Pantzaris Marios , Ioannou Melina , S. Patrikios Ioannis , D. Giannaki Christoforos
Published: January 2020

The aim of the present study was to examine the effects of a high-dose omega-3 and omega-6 fatty acids supplementation, in combination with antioxidant vitamins, on cognitive function and functional capacity of older adults with mild cognitive impairment (MCI), over a 6-month period in a randomized, double-blind, placebo-controlled trial. Forty-six older adults with MCI (age: 78.8 ± 7.3 years) were randomized 1:1 to receive either a 20 mL dose of a formula containing a mixture of omega-3 (810 mg Eicosapentaenoic acid and 4140 mg Docosahexaenoic acid) and omega-6 fatty acids (1800 mg gamma-Linolenic acid and 3150 mg Linoleic acid) (1:1 w/w), with 0.6 mg vitamin A, vitamin E (22 mg) plus pure γ-tocopherol (760 mg), or 20 mL placebo containing olive oil. Participants completed assessments of cognitive function, functional capacity, body composition and various aspects of quality of life at baseline and following three and six months of supplementation. Thirty-six participants completed the study (eighteen from each group). A significant interaction between supplementation and time was found on cognitive function (Addenbrooke’s Cognitive Examination -Revised (ACE-R), Mini-Mental State Examination (MMSE) and Stroop Color and Word Test (STROOP) color test; p < 0.001, p = 0.011 and p = 0.037, respectively), functional capacity (6-min walk test and sit-to-stand-60; p = 0.028 and p = 0.032, respectively), fatigue (p < 0.001), physical health (p = 0.007), and daily sleepiness (p = 0.007)—showing a favorable improvement for the participants receiving the supplement. The results indicate that this nutritional modality could be promising for reducing cognitive and functional decline in the elderly with MCI.
Food Combinations in Relation to the Quality of Overall Diet and Individual Meals in Japanese Adults: A Nationwide Study
Authors: Murakami Kentaro , Livingstone M. Barbara E. , Shinozaki Nana , Sugimoto Minami , Fujiwara Aya , Masayasu Shizuko , Sasaki Satoshi
Published: January 2020

We examined food combinations in relation to the quality of the overall diet and individual meals using a newly developed food combination questionnaire (FCQ) in a nationwide sample of Japanese adults aged 19–80 years (n = 2233). The quality of the overall diet and of each meal was assessed by the Healthy Eating Index-2015 (HEI-2015) and Nutrient-Rich Food Index 9.3 (NRF9.3). For all main meals (breakfast, lunch, and dinner), the most commonly consumed food combinations consisted of ‘rice, total vegetables, and tea and coffee’. Consistently positive associations between these food combinations and diet quality were found for breakfast (Spearman r: ≥0.46). Positive rather weak associations between these food combinations and diet quality were also observed for lunch (Spearman r: ≤0.48). Conversely, the associations were inconsistent for dinner: inverse associations with HEI-2015 (Spearman r: ≤−0.35) and generally weak positive associations with NRF9.3 (Spearman r: ≥0.09). For snacks, the most commonly consumed food combinations consisted of ‘confectioneries and tea and coffee’, but these showed rather weak associations with diet quality. Similar results were obtained when associations with the quality of overall diet were investigated. The FCQ may be useful in capturing the complex nature of food combinations in Japanese adults.
Associations between Triglyceride-Glucose Index and Micro- and Macro-Angiopathies in Type 2 Diabetes Mellitus
Authors: Chiu Hsuan , Tsai Hui-Ju , Huang Jiun-Chi , Wu Pei-Yu , Hsu Wei-Hao , Lee Mei-Yueh , Chen Szu-Chia
Published: January 2020

The aim of this study was to investigate the associations between the triglyceride-glucose (TyG) index, a marker of insulin resistance, and microangiopathies and macroangiopathies in patients with type 2 diabetes mellitus (DM). Our study enrolled 1990 type 2 diabetic patients from local hospitals in Taiwan during the period of 2002–2004. Multivariate logistic regression analysis was used to identify the factors related to microangiopathies, macroangiopathiess and TyG index. The patients were stratified by TyG index quartile (Q1–Q4). Adjusted odds ratios (aORs) of albuminuria for Q3 versus Q1 and Q4 versus Q1 were 1.424 (95% confidence interval [CI], 1.062–1.910, p = 0.018) and 1.948 (95% CI, 1.437–2.642, p < 0.001), respectively. The aOR of cerebrovascular disease (CVA) was 2.264 for Q4 versus Q1 (95% CI, 1.243–4.122, p = 0.008), but there were no significant associations with diabetic retinopathy (DR), coronary artery disease (CAD) or peripheral artery occlusive disease (PAOD). Significant stepwise increases were found in CVA (p = 0.0013) and albuminuria ≥30 mg/g (p < 0.001) corresponding to TyG index quartiles, but not DR (p = 0.573), CAD (p = 0.880) or PAOD (p = 0.223). There was a significant association between a high TyG index and a high risk of microalbuminuria and CVA in the study cohort. Further studies to investigate the use of medications in patients with DM to prevent micro- and macro-angiopathies are thus warranted.
Improving Nutrition Information in the Eastern Mediterranean Region: Implementation of Front-of-Pack Nutrition Labelling
Authors: Al-Jawaldeh Ayoub , Rayner Mike , Julia Chantal , Elmadfa Ibrahim , Hammerich Asmus , McColl Karen
Published: January 2020

The provision of simplified nutrition information, in a prominent place on the front of food packages, is recommended as an important element of comprehensive strategies to tackle the burden of death and disease caused by unhealthy diets. There is growing evidence that front-of-pack nutrition labels are preferred by consumers, are more likely to be looked at or noticed than nutrition labelling on the back or side of packages and can help consumers to better identify healthier and less healthy products. This review summarizes current implementation of front-of-pack nutrition labelling policies in the countries of the WHO Eastern Mediterranean Region. Implementation of front-of-pack nutrition labelling in the Eastern Mediterranean Region remains limited, but three types of scheme were identified as having been implemented or at an advanced stage of development by governments in six countries. Through a review of reviews of existing research and evidence from country implementation, the authors suggest some pointers for implementation for other countries in the Region deciding to implement front-of-pack nutrition labelling policies.
Knowledge and Attitudes Towards Nutrigenetics: Findings from the 2019 Unified Forces Preventive Nutrition Conference (UFPN)
Authors: Kaufman-Shriqui Vered , Salem Hagit , Boaz Mona , Birk Ruth
Published: January 2020

Background: Nutrigenetics indicates that individual genetic variability results in altered health outcomes necessitating personalized nutrition adaptation. Registered dietitians are recognized as the clinical nutrition experts, but their knowledge and attitudes regarding nutrigenetics has not been delineated. Methods: This cross sectional online survey was conducted in a convenience sample of 169 national nutrition conference attendees. The survey queried demographics, knowledge, and attitudes towards nutrigenetics and information on training in nutrigenetics. Results: The majority of participants were registered dietitians and female, 45% of whom held advanced degrees. Personalized nutrition was perceived by 93.5% of participants as highly important or important; however, 94% of respondents indicated they are not sufficiently knowledgeable in personalized nutrition and only 9.5% had received training in nutrigenetics. The mean nutrigenetics knowledge score was 6.89 ± 1.67 (out of a possible 12). A multivariate regression model of knowledge score identified education as the only independent predictor of this outcome. Conclusion: Personalized nutrition is a rapidly developing field that incorporates genetic data into clinical practice. Dietitians recognize the importance of advanced studies to acquire knowledge in nutrigenetics. Only by acquiring the necessary knowledge can dietitians accurately translate this nutrigenetics into clinical practice.
Antiobesity Effects of Extract from Spergularia marina Griseb in Adipocytes and High-Fat Diet-Induced Obese Rats
Authors: Park Yong-Hyun , Lee Jae-Joon , Son Hee-Kyoung , Kim Bok-Hee , Byun Jaemin , Ha Jung-Heun
Published: January 2020

Obesity has recently risen and become a serious health concern in Korea according to the westernized diet and altered lifestyle. Hence, there is a growing interest in the supplementation of phytochemicals to find a safe and effective functional ingredient to treat obesity. Spergularia marina Griseb (SM) has traditionally been used as a natural herb against chronic diseases in Korea. In this study, we investigated the antiobesity effects of SM in vitro and in vivo. SM ethanol extract (SME) inhibited proliferation and differentiation in murine adipocytes and primary porcine pre-adipocytes in a dose-dependent manner. In the in vivo study, supplementation of SM powder (SMP) remarkably attenuated fat accumulation in HFD-induced obese rats. In addition, SMP supplementation improved lipid profiles in the serum and tissues of high-fat induced obese rats. Collectively, these data indicated that SME exhibited antiobesity effects by modulating adipogenesis and lipolysis. Furthermore, SMP could be developed as an obesity-induced metabolic syndrome treatment.
Metal Organic Framework@Polysilsesequioxane Core/Shell-Structured Nanoplatform for Drug Delivery
Authors: Lu Liangyu , Ma Mengyu , Gao Chengtao , Li Hongwei , Li Long , Dong Fuping , Xiong Yuzhu
Published: January 2020

Modern pharmaceutics requires novel drug loading platforms with high drug loading capacity, controlled release, high stability, and good biocompacity. Metal–organic frameworks (MOFs) show promising applications in biomedicine owing to their extraordinarily high surface area, tunable pore size, and adjustable internal surface properties. However, MOFs have low stability due to weak coordinate bonding and limited biocompatibility, limiting their bioapplication. In this study, we fabricated MOFs/polysilsesquioxane (PSQ) nanocomposites and utilized them as drug carriers. Amine-functionalized MOF (UiO-66-NH2) nanoparticles were synthesized and encapsulated with epoxy-functionalized polysilsesquioxane layer on the surface via a facile process. MOFs possessed high surface area and regular micropores, and PSQs offered stability, inertness, and functionality. The obtained UiO-66-NH2@EPSQ nanocomposites were utilized as carriers for ibuprofen, a drug with carboxylic groups on the surface, and demonstrated high drug loading capacity and well-controlled release property. The UiO-66-NH2@EPSQ nanocomposite exhibited low cytotoxicity to HeLa cells within a wide concentration range of 10–100 µg/mL, as estimated by the MTT method. The UiO-66-NH2@EPSQ drug release system could be a potential platform in the field of controlled drug delivery.
Exploring the Link between Photosystem II Assembly and Translation of the Chloroplast psbA mRNA
Authors: Chotewutmontri Prakitchai , Williams-Carrier Rosalind , Barkan Alice
Published: January 2020

Photosystem II (PSII) in chloroplasts and cyanobacteria contains approximately fifteen core proteins, which organize numerous pigments and prosthetic groups that mediate the light-driven water-splitting activity that drives oxygenic photosynthesis. The PSII reaction center protein D1 is subject to photodamage, whose repair requires degradation of damaged D1 and its replacement with nascent D1. Mechanisms that couple D1 synthesis with PSII assembly and repair are poorly understood. We address this question by using ribosome profiling to analyze the translation of chloroplast mRNAs in maize and Arabidopsis mutants with defects in PSII assembly. We found that OHP1, OHP2, and HCF244, which comprise a recently elucidated complex involved in PSII assembly and repair, are each required for the recruitment of ribosomes to psbA mRNA, which encodes D1. By contrast, HCF136, which acts upstream of the OHP1/OHP2/HCF244 complex during PSII assembly, does not have this effect. The fact that the OHP1/OHP2/HCF244 complex brings D1 into proximity with three proteins with dual roles in PSII assembly and psbA ribosome recruitment suggests that this complex is the hub of a translational autoregulatory mechanism that coordinates D1 synthesis with need for nascent D1 during PSII biogenesis and repair.
Community Structure, Diversity and Potential of Endophytic Bacteria in the Primitive New Zealand Medicinal Plant Pseudowintera colorata
Authors: Purushotham Neeraj , Jones Eirian , Monk Jana , Ridgway Hayley
Published: January 2020

Although the importance of the plant microbiome in commercial plant health has been well established, there are limited studies in native medicinal plants. Pseudowintera colorata (horopito) is a native New Zealand medicinal plant recognized for its antimicrobial properties. Denaturing gradient gel electrophoresis (DGGE) and Illumina MiSeq analysis of P. colorata plants from ten sites across New Zealand showed that tissue type strongly influenced the diversity and richness of endophytic bacteria (PERMANOVA, P < 0.05). In addition, two OTUs belonging to the genus Pseudomonas (Greengenes ID: 646549 and 138914) were found to be present in >75% of all P. colorata leaf, stem and root samples and were identified as the members of the P. colorata “core endomicrobiome”. Culture-independent analysis was complemented by the recovery of 405 endophytic bacteria from the tissues of P. colorata. Some of these cultured endophytic bacteria (n = 10) showed high antagonism against four different phytopathogenic fungi tested. The influence of endophytic bacteria on plant growth was assessed by inoculating P. colorata seedlings. The mean shoot height of seedlings treated with Bacillus sp. TP1LA1B were longer (1.83×), had higher shoot dry weight (1.8×) and produced more internodes (1.8×) compared to the control.
The Role of Nanoparticle Shapes and Structures in Material Characterisation of Polyvinyl Alcohol (PVA) Bionanocomposite Films
Authors: Mousa Mohanad , Dong Yu
Published: January 2020

Three different types of nanoparticles, 1D Cloisite 30B clay nanoplatelets, 2D halloysite nanotubes (HNTs), and 3D nanobamboo charcoals (NBCs) were employed to investigate the impact of nanoparticle shapes and structures on the material performance of polyvinyl alcohol (PVA) bionanocomposite films in terms of their mechanical and thermal properties, morphological structures, and nanomechanical behaviour. The overall results revealed the superior reinforcement efficiency of NBCs to Cloisite 30B clays and HNTs, owing to their typical porous structures to actively interact with PVA matrices in the combined formation of strong mechanical and hydrogen bondings. Three-dimensional NBCs also achieved better nanoparticle dispersibility when compared with 1D Cloisite 30B clays and 2D HNTs along with higher thermal stability, which was attributed to their larger interfacial regions when characterised for the nanomechanical behaviour of corresponding bionanocomposite films. Our study offers an insightful guidance to the appropriate selection of nanoparticles as effective reinforcements and the further sophisticated design of bionanocomposite materials.
Anaerobic Digestate Treatment Selection Model for Biogas Plant Costs and Emissions Reduction
Authors: Đurđević Dinko , Hulenić Ivona
Published: January 2020

Agriculture is one of the leading sectors on the global level contributing to greenhouse gas (GHG) emissions increase. With the utilization of biogas production technology within the agriculture sector, ecological benefits could be achieved, with immediate economic profit. Therefore, to retain economic profit and environmental sustainability, implementation of bioeconomy principles is of key importance. This paper examines four options of digestate treatment, which is identified as one of the highest operational cost for the biogas plant. A simple and robust model in Excel Solver was developed to determine the best solution for minimising GHG emissions and maximise profit for the biogas plant operator, through an upgrade of the plant with digestate treatment technologies. The model was implemented on a case of a Croatian biogas plant and the best solution in terms of GHG reduction and profit increase proved to be fertilizer production (Option 1), through a crystallization process of struvite within the digestate. This option obtains a significant reduction in GHG emissions compared to standard biogas production without additional upgrades (Option 4), by over 90%, and increase of profit for the biogas plant operator, which diversifies the income source and creates multiple positive impacts on the environment.
A Numerical Study on the Flow Mechanism of Performance Improvement of a Wide-Angle Diffuser by Inserting a Short Splitter Vane
Authors: Meng Xu , Zuo Zhigang , Nishi Michihiro , Liu Shuhong
Published: January 2020

Usage of a wide-angle diffuser may result in unfavorable separated flow and a significant diffuser loss. To improve the performance of the diffusers, inserting short splitter vanes is known as a useful method that has been demonstrated experimentally. Regarding the role of the vane in the diffuser flow, Senoo & Nishi (1977) qualitatively explained that the lift force acting on the vane should be a key factor. However, its quantitative verification remains since then. To challenge this issue, numerical simulations of incompressible flow in a wide angle of 28° two-dimensional diffuser with and without a short splitter vane were conducted in the present study. An improvement of pressure-recovery by the vane and oscillatory flows in the diffuser are reasonably reproduced from comparison with the experimental results made by Cochran & Kline (1958). It is also found that the lift force acting on the vane varies periodically in an opposite phase with the detachment point moved back and forth on a diverging wall, since one vane is not sufficient to fully suppress the flow separation that occurred on the wall and the incoming main-flow shifts toward the other diverging wall in the diffuser. Thus, as a role of splitter vane in the diffuser, “the lift force of the vane is a key factor” may be quantitatively verified from the present numerical simulation. Further, it is confirmed by the local loss analysis that the turbulent kinetic energy production observed in mixing layers contributes most of the loss in the diffuser. Consequently, the present numerical technique may be usable to investigate the flow character in a diffuser with splitter vanes at a design stage.
CFD Simulation of Forced Recirculating Fired Heated Reboilers
Authors: Davidy Alon
Published: January 2020

An advanced algorithm has been developed in order to analyze the performance of re-boiling process of crude oil flowing inside reboilers tubes. The proposed model is composed from Heptane fire heater and a tube array. The heat flux produced from burner is transferred to the crude oil flowing inside the tube. The computational model is composed of two phases—Simulation of fire by using Fire Dynamics Simulator software (FDS) version 5.0 and then a nucleate boiling computation of the crude oil. FDS code is formulated based on CFD (Computational Fluid Dynamics) of fire heater. The thermo-physical properties (such as: thermal conductivity, heat capacity, surface tension, viscosity) of the crude oil were estimated by using empirical correlations. The thermal heat transfer to evaporating two-phase crude oil mixture occur by bubble generation at the wall (nucleate boiling) has been calculated by using Chen correlation. It has been assumed that the overall convective heat transfer coefficient is composed from the nucleate boiling convective coefficient and the forced turbulent convective coefficient. The former is calculated by Forster Zuber empirical equation. The latter is computed from the Dittus-Boelter relationship. In order to validate the nucleate boiling heat transfer coefficient, a comparison has been performed to nucleate boiling convective coefficient obtained by Mostinski equation. The relative error between the nucleate boiling convective heat-transfer coefficients is 10.5%. The FDS numerical solution has been carried out by using Large Eddy Simulation (LES) method. This work has been further extended to include also the structural integrity aspects of the reboiler metal pipe by using COMSOL Multiphysics software. It was found out, that the calculated stress is less than the ultimate tensile strength of the AISI 310 Steel alloy.
Liquid Biphasic System: A Recent Bioseparation Technology
Authors: Khoo Kuan Shiong , Leong Hui Yi , Chew Kit Wayne , Lim Jun-Wei , Ling Tau Chuan , Show Pau Loke , Yen Hong-Wei
Published: January 2020

A well-known bioseparation technique namely liquid biphasic system (LBS) has attracted many researchers’ interest for being an alternative bioseparation technology for various kinds of biomolecules. The present review begins with an in-depth discussion on the fundamental principle of LBS and this is followed by the discussion on further development of various phase-forming components in LBS. Additionally, the implementation of various advance technologies to the LBS that is beneficial towards the efficiency of LBS for the extraction, separation, and purification of biomolecules was discussed. The key parameters affecting the LBS were presented and evaluated. Moreover, future prospect and challenges were highlighted to be a useful guide for future development of LBS. The efforts presented in this review will provide an insight for future researches in liquid-liquid separation techniques.
Correction: Vachaparambil, K.J. Comparison of Surface Tension Models for the Volume of Fluid Method. Processes 2019, 7, 542
Authors: Vachaparambil Kurian J. , Einarsrud Kristian Etienne
Published: January 2020

In Equations (2) and (3) [...]
Possibility to Save Water and Energy by Application of Fresh Vegetables to Produce Supplemented Potato-Based Snack Pellets
Authors: Lisiecka Katarzyna , Wójtowicz Agnieszka
Published: January 2020

The aim of the study was to examine the effect of fresh vegetable addition on processing efficiency, and to ascertain the energy and water consumption during production of potato-based snack pellets. The extrusion-cooking process with a modified single screw extruder was applied at variable screw speeds and amounts of vegetable additives. A mixture of potato flakes, potato grits and starch was used as a basic recipe. The potato composition was supplemented with fresh pulp of onion, leek, kale and carrot in amounts of 2.5–30.0% as replacement of a related amount of potato starch. The water consumption, as well as processing indicators: the production efficiency, the specific mechanical energy (SME), and the total SME requirements during snack pellets processing at the laboratory scale were evaluated. As a result of this work, we found that the amount of applied vegetable additives had little impact on both processing efficiency and SME depending on the screw speeds applied. Moreover, we saw increased processing efficiency with increased screw speed during extrusion. Of particular note, maximum value of processing efficiency was observed if fresh onion was used as an additive at the highest speed screw. Furthermore, the lowest specific mechanical energy consumption was noted for extrudates supplemented with fresh onion addition processed at the lowest screw speed. The most important limiting of water consumption during processing without negative effects on processing efficiency and quality of the final snack pellets was observed if 20% to 30% of fresh vegetables were used in the recipe. We believe that application of fresh vegetable pulp limited the energy requirements by mitigating the drying process of additives.
Improved Removal of Quinoline from Wastewater Using Coke Powder with Inorganic Ions
Authors: Wang Lei , Gao Qieyuan , Li Zhipeng , Wang Yongtian
Published: January 2020

In this paper, laboratory batch adsorption tests were performed to study the adsorption behavior of coke powder in a quinoline aqueous solution with the absence and presence of inorganic ions (K+ and Ca2+). Adsorption isotherms, thermodynamic parameters, and kinetic models were used to understand the sorption mechanism, and zeta potential measurements were performed to elucidate the effect of the inorganic ions on the adsorption. The results showed that coke powder exhibited a reasonably good adsorption performance due to its pore structure and surface characteristics, and the presence of K+ and Ca2+ could further improve the adsorption. Without inorganic ions, the adsorption capacity of coke powder for quinoline and the removal efficiency of quinoline were 1.27 mg/g and 84.90%, respectively. At the ion concentration of 15 mmol, the adsorption capacity of coke powder and quinoline removal efficiency in the presence of K+ reached 1.38 mg/g and 92.02%, respectively, whereas those in the solutions with Ca2+ reached 1.40 mg/g and 93.31%, respectively. It was found that the adsorption of quinoline onto coke powder in the absence and presence of inorganic ions fit the Freundlich isotherm. Changes in the Gibbs free energy, the heat of adsorption, the entropy, and the activation energy of adsorption suggest that the adsorption was spontaneous and exothermic, which was dominated by physical adsorption, and that the added K+ and Ca2+ would favor the adsorption. In addition, the pseudo-second-order kinetic model was found to provide the best fit to the adsorption kinetic data, and K+ and Ca2+ increased the rate of quinoline adsorbed onto coke power. This improved adsorption due to inorganic ions was found to be a consequence of the decrease in the surface potential of coke powder particles, which resulted in a reduced thickness of water film around particles, as well as a decreased electrostatic repulsion between coke powder particles and quinoline molecules.
Detection of Aquatic Plants Using Multispectral UAV Imagery and Vegetation Index
Authors: Song Bonggeun , Park Kyunghun
Published: January 2020

In this study, aquatic plants in a small reservoir were detected using multispectral UAV (Unmanned Aerial Vehicle) imagery and various vegetation indices. A Firefly UAV, which has both fixed-wing and rotary-wing flight modes, was flown over the study site four times. A RedEdge camera was mounted on the UAV to acquire multispectral images. These images were used to analyze the NDVI (Normalized Difference Vegetation Index), ENDVI (Enhance Normalized Difference Vegetation Index), NDREI (Normalized Difference RedEdge Index), NGRDI (Normalized Green-Red Difference Index), and GNDVI (Green Normalized Difference Vegetation Index). As for multispectral characteristics, waterside plants showed the highest reflectance in Rnir, while floating plants had a higher reflectance in Rre. During the hottest season (on 25 June), the vegetation indices were the highest, and the habitat expanded near the edge of the reservoir. Among the vegetation indices, NDVI was the highest and NGRDI was the lowest. In particular, NGRDI had a higher value on the water surface and was not useful for detecting aquatic plants. NDVI and GNDVI, which showed the clearest difference between aquatic plants and water surface, were determined to be the most effective vegetation indices for detecting aquatic plants. Accordingly, the vegetation indices using multispectral UAV imagery turned out to be effective for detecting aquatic plants. A further study will be accompanied by a field survey in order to acquire and analyze more accurate imagery information.
X-Band Radar for Cetacean Detection (Focus on Tursiops truncatus) and Preliminary Analysis of Their Behavior
Authors: Mingozzi Matteo , Salvioli Francesca , Serafino Francesco
Published: January 2020

Cetaceans are protected species all over the world, most of them are vulnerable, endangered, or data deficient (according to International Union for Conservation of Nature - IUCN red list). X-band radars detect the echo of the electromagnetic signal reflected by an obstacle or a ship (target). The application of X-band radar to the detection of cetaceans is a new and innovative field of research that could improve the automation of marine mammal data collection, and this is the first time in the Mediterranean Sea. The aim of this work was to test the capability of X-band radar installed along the coast (ground-based) to detect and track cetaceans in a range of approximately 2.5 nautical miles from the radar antenna. Data collection included a part of field work, implemented through the acquisition of photographic images and target’s radar detection (by the panoramic terrace Santa Maria in Corniglia), and a part, performed in the laboratory, of data analysis. The work was undertaken between May and November 2018. During this period, 30 days of monitoring were carried out (about 300 h) and about 10,000 radar images were recorded. The first results showed that we were able to recognize the target “cetacean” from the other common targets (boats, buoys, etc.) detected by the radar. In particular 70 dolphins were sighted by visual census; 12 of them were recognized on radar images. Radar images allowed extraction of dolphin dive time (between 2 and 15 s). The next step will be to allow the radar to identify the presence of marine mammals itself since it also works at night and with low visibility. This technique could complement the protection measures of cetaceans, highlighting their presence at sea even if it is impossible with waves higher than 0.8 m and over distances greater than 2.5 km.