bluuebunny/crossref_metadata_2025 huggingface dataset:
A Universal System of CRISPR/Cas9-Mediated Gene Targeting Using All-in-One Vector in Plants
Authors: Nishizawa-Yokoi Ayako , Mikami Masafumi , Toki Seiichi
Published: November 2020

Homologous recombination-mediated genome editing, also called gene targeting (GT), is an essential technique that allows precise modification of a target sequence, including introduction of point mutations, knock-in of a reporter gene, and/or swapping of a functional domain. However, due to its low frequency, it has been difficult to establish GT approaches that can be applied widely to a large number of plant species. We have developed a simple and universal clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9)-mediated DNA double-strand break (DSB)-induced GT system using an all-in-one vector comprising a CRISPR/Cas9 expression construct, selectable marker, and GT donor template. This system enabled introduction of targeted point mutations with non-selectable traits into several target genes in both rice and tobacco. Since it was possible to evaluate the GT frequency on endogenous target genes precisely using this system, we investigated the effect of treatment with Rad51-stimulatory compound 1 (RS-1) on the frequency of DSB-induced GT. GT frequency was slightly, but consistently, improved by RS-1 treatment in both target plants.
Targeting Opposing Immunological Roles of the Junctional Adhesion Molecule-A in Autoimmunity and Cancer
Authors: Bonilha Caio S. , Benson Robert A. , Brewer James M. , Garside Paul
Published: November 2020

The junctional adhesion molecule-A (JAM-A) is a cell surface adhesion molecule expressed on platelets, epithelial cells, endothelial cells and leukocytes (e. g. monocytes and dendritic cells). JAM-A plays a relevant role in leukocyte trafficking and its therapeutic potential has been studied in several pathological conditions due to its capacity to induce leukocyte migration out of inflamed sites or infiltration into tumor sites. However, disruption of JAM-A pathways may worsen clinical pathology in some cases. As such, the effects of JAM-A manipulation on modulating immune responses in the context of different diseases must be better understood. In this mini-review, we discuss the potential of JAM-A as a therapeutic target, summarizing findings from studies manipulating JAM-A in the context of inflammatory diseases (e.g. autoimmune diseases) and cancer and highlighting described mechanisms.
Risk Factors for Patient–Ventilator Asynchrony and Its Impact on Clinical Outcomes: Analytics Based on Deep Learning Algorithm
Authors: Ge Huiqing , Duan Kailiang , Wang Jimei , Jiang Liuqing , Zhang Lingwei , Zhou Yuhan , Fang Luping , Heunks Leo M. A. , Pan Qing , Zhang Zhongheng
Published: November 2020

Background and objectives: Patient–ventilator asynchronies (PVAs) are common in mechanically ventilated patients. However, the epidemiology of PVAs and its impact on clinical outcome remains controversial. The current study aims to evaluate the epidemiology and risk factors of PVAs and their impact on clinical outcomes using big data analytics.

Methods: The study was conducted in a tertiary care hospital; all patients with mechanical ventilation from June to December 2019 were included for analysis. Negative binomial regression and distributed lag non-linear models (DLNM) were used to explore risk factors for PVAs. PVAs were included as a time-varying covariate into Cox regression models to investigate its influence on the hazard of mortality and ventilator-associated events (VAEs).

Results: A total of 146 patients involving 50,124 h and 51,451,138 respiratory cycles were analyzed. The overall mortality rate was 15.6%. Double triggering was less likely to occur during day hours (RR: 0.88; 95% CI: 0.85–0.90; p < 0.001) and occurred most frequently in pressure control ventilation (PCV) mode (median: 3; IQR: 1–9 per hour). Ineffective effort was more likely to occur during day time (RR: 1.09; 95% CI: 1.05–1.13; p < 0.001), and occurred most frequently in PSV mode (median: 8; IQR: 2–29 per hour). The effect of sedatives and analgesics showed temporal patterns in DLNM. PVAs were not associated mortality and VAE in Cox regression models with time-varying covariates.

Conclusions: Our study showed that counts of PVAs were significantly influenced by time of the day, ventilation mode, ventilation settings (e.g., tidal volume and plateau pressure), and sedatives and analgesics. However, PVAs were not associated with the hazard of VAE or mortality after adjusting for protective ventilation strategies such as tidal volume, plateau pressure, and positive end expiratory pressure (PEEP).
Improving the Quality of Dementia Care in General Practice: A Qualitative Study
Authors: Bourque Meghan , Foley Tony
Published: November 2020

Background: General Practitioners (GPs) play a central role in caring for people with dementia. There is a growing demand for GP-led community-based dementia care, as advocated in the Irish National Dementia Strategy (INDS). However, there is a paucity of research exploring GPs' views on dementia care since publication of the INDS. The aim of this qualitative study is to develop a deeper understanding of how to improve the quality of dementia care in General Practice, explored from the perspective of Irish GPs.

Methods: Semi-structured interviews were conducted with GPs. GPs who completed the “Dementia in Primary Care” CPD module at University College Cork in Ireland were purposively recruited. Interviews were audio-recorded, transcribed, and analyzed by thematic analysis.

Results: 12 interviews were conducted with 7 female and 5 male participants. Experience in General Practice ranged from 3 to 32 years. Most GPs practiced in mixed urban-rural settings (n = 9) and had nursing home commitments (n = 8). The average interview length was 45 minutes. Six major themes emerged from the data set, including resourcing primary care, addressing disparities in secondary care, community-centered care as patient-centered care, linking a dementia network, universal access to care, and raising public awareness.

Conclusion: GPs find dementia care to be a complex and challenging aspect of primary care. While education and training is advocated by GPs, service delivery must be reconfigured. This will necessitate adequate financial resourcing and the restructuring of community-based dementia care services.
Engineered Lysins With Customized Lytic Activities Against Enterococci and Staphylococci
Authors: Binte Muhammad Jai Hana Sakina , Dam Linh Chi , Tay Lowella Servito , Koh Jodi Jia Wei , Loo Hooi Linn , Kline Kimberly A. , Goh Boon Chong
Published: November 2020

The emergence of multidrug-resistant bacteria has made minor bacterial infections incurable with many existing antibiotics. Lysins are phage-encoded peptidoglycan hydrolases that have demonstrated therapeutic potential as a novel class of antimicrobials. The modular architecture of lysins enables the functional domains – catalytic domain (CD) and cell wall binding domain (CBD) – to be shuffled to create novel lysins. The CD is classically thought to be only involved in peptidoglycan hydrolysis whereas the CBD dictates the lytic spectrum of a lysin. While there are many studies that extended the lytic spectrum of a lysin by domain swapping, few have managed to introduce species specificity in a chimeric lysin. In this work, we constructed two chimeric lysins by swapping the CBDs of two parent lysins with different lytic spectra against enterococci and staphylococci. We showed that these chimeric lysins exhibited customized lytic spectra distinct from the parent lysins. Notably, the chimeric lysin P10N-V12C, which comprises a narrow-spectrum CD fused with a broad-spectrum CBD, displayed species specificity not lysingEnterococcus faeciumwhile targetingEnterococcus faecalisand staphylococci. Such species specificity can be attributed to the narrow-spectrum CD of the chimeric lysin. Using flow cytometry and confocal microscopy, we found that theE. faeciumcells that were treated with P10N-V12C are less viable with compromised membranes yet remained morphologically intact. Our results suggest that while the CBD is a major determinant of the lytic spectrum of a lysin, the CD is also responsible in the composition of the final lytic spectrum, especially when it pertains to species-specificity.
Characterization of the Prognostic Values of the CXCR1-7 in Clear Cell Renal Cell Carcinoma (ccRCC) Microenvironment
Authors: Wu Zhulin , Zhang Yingzhao , Chen Xiang , Tan Wanjun , He Li , Peng Lisheng
Published: November 2020

Background:As cancer immunotherapy has become a hot research topic, the values of CXC chemokine receptors (CXCRs) in tumor microenvironment have been increasingly realized. More and more evidence showed that the aberrant expression of CXCRs is closely related to the prognosis of various cancers. However, prognostic values and the exact roles of different CXCRs in clear cell renal cell carcinoma (ccRCC) have not yet been elucidated.

Methods:To further evaluate the potential of seven CXCRs as prognostic biomarkers for ccRCC, multiple online analysis tools, including ONCOMINE, UALCAN (TCGA dataset), Kaplan–Meier Plotter, MethSurv, cBioPortal, GEPIA, Metascape, and TIMER databases, were utilized in our research.

Results:The mRNA expression of CXCR4/6/7 was significantly increased in ccRCC patients, and all CXCRs are remarkably related to tumor stage or grade of ccRCC. Higher levels of CXCR3/4/5/6 expression were correlated with worse overall survival (OS) in patients with ccRCC, while higher expression of CXCR2 was associated with better OS. 23.14% mutation rate (118/510) of CXCR1-7 was observed in ccRCC patients, and the genetic alterations in CXCRs were related to worse OS and progression-free survival in ccRCC patients. Additionally, 53 CpGs of CXCR1-7 showed significant prognostic values. For functional enrichment, our results showed that CXCRs and their similar genes may be involved in cancer-associated pathways, immune process, and angiogenesis, etc. Besides, CXCRs were significantly correlated with multiple immune cells (e.g., CD8+ T cell, CD4+ cell, and dendritic cell).

Conclusion:This study explored the potential prognostic values and roles of the CXCRs in ccRCC microenvironment. Our results suggested that CXCR4 and CXCR6 could be the prognostic biomarkers for the patients with ccRCC.
Mechanistic Understanding From Molecular Dynamics Simulation in Pharmaceutical Research 1: Drug Delivery
Authors: Bunker Alex , Róg Tomasz
Published: November 2020

In this review, we outline the growing role that molecular dynamics simulation is able to play as a design tool in drug delivery. We cover both the pharmaceutical and computational backgrounds, in a pedagogical fashion, as this review is designed to be equally accessible to pharmaceutical researchers interested in what this new computational tool is capable of and experts in molecular modeling who wish to pursue pharmaceutical applications as a context for their research. The field has become too broad for us to concisely describe all work that has been carried out; many comprehensive reviews on subtopics of this area are cited. We discuss the insight molecular dynamics modeling has provided in dissolution and solubility, however, the majority of the discussion is focused on nanomedicine: the development of nanoscale drug delivery vehicles. Here we focus on three areas where molecular dynamics modeling has had a particularly strong impact: (1) behavior in the bloodstream and protective polymer corona, (2) Drug loading and controlled release, and (3) Nanoparticle interaction with both model and biological membranes. We conclude with some thoughts on the role that molecular dynamics simulation can grow to play in the development of new drug delivery systems.
Applications of Non-invasive Neuromodulation for the Management of Disorders Related to COVID-19
Authors: Baptista Abrahão Fontes , Baltar Adriana , Okano Alexandre Hideki , Moreira Alexandre , Campos Ana Carolina Pinheiro , Fernandes Ana Mércia , Brunoni André Russowsky , Badran Bashar W. , Tanaka Clarice , de Andrade Daniel Ciampi , da Silva Machado Daniel Gomes , Morya Edgard , Trujillo Eduardo , Swami Jaiti K. , Camprodon Joan A. , Monte-Silva Katia , Sá Katia Nunes , Nunes Isadora , Goulardins Juliana Barbosa , Bikson Marom , Sudbrack-Oliveira Pedro , de Carvalho Priscila , Duarte-Moreira Rafael Jardim , Pagano Rosana Lima , Shinjo Samuel Katsuyuki , Zana Yossi
Published: November 2020

Background: Novel coronavirus disease (COVID-19) morbidity is not restricted to the respiratory system, but also affects the nervous system. Non-invasive neuromodulation may be useful in the treatment of the disorders associated with COVID-19.

Objective: To describe the rationale and empirical basis of the use of non-invasive neuromodulation in the management of patients with COVID-10 and related disorders.

Methods: We summarize COVID-19 pathophysiology with emphasis of direct neuroinvasiveness, neuroimmune response and inflammation, autonomic balance and neurological, musculoskeletal and neuropsychiatric sequela. This supports the development of a framework for advancing applications of non-invasive neuromodulation in the management COVID-19 and related disorders.

Results: Non-invasive neuromodulation may manage disorders associated with COVID-19 through four pathways: (1) Direct infection mitigation through the stimulation of regions involved in the regulation of systemic anti-inflammatory responses and/or autonomic responses and prevention of neuroinflammation and recovery of respiration; (2) Amelioration of COVID-19 symptoms of musculoskeletal pain and systemic fatigue; (3) Augmenting cognitive and physical rehabilitation following critical illness; and (4) Treating outbreak-related mental distress including neurological and psychiatric disorders exacerbated by surrounding psychosocial stressors related to COVID-19. The selection of the appropriate techniques will depend on the identified target treatment pathway.

Conclusion: COVID-19 infection results in a myriad of acute and chronic symptoms, both directly associated with respiratory distress (e.g., rehabilitation) or of yet-to-be-determined etiology (e.g., fatigue). Non-invasive neuromodulation is a toolbox of techniques that based on targeted pathways and empirical evidence (largely in non-COVID-19 patients) can be investigated in the management of patients with COVID-19.
Effects of Visual Attentional Load on the Tactile Sensory Memory Indexed by Somatosensory Mismatch Negativity
Authors: He Xin , Zhang Jian , Zhang Zhilin , Go Ritsu , Wu Jinglong , Li Chunlin , Gan Kai , Chen Duanduan
Published: November 2020

Auditory sensory memory indexed by mismatch negativity has been broadly studied over the past century, but far less attention has been directed to tactile sensory memory. To investigate whether tactile sensory memory is affected by attention, we recorded somatosensory mismatch negativity (sMMN) from 24 healthy adults in two experiments to distinguish sustained attention from non-sustained attention. Using the roving somatosensory oddball paradigm, we analyzed the average dynamic changes in the amplitude and latency of sMMN amplitude and found a clear sMMN component at the central region at a 100–300 ms interval. The sMMN amplitude, which indexes the early detection of tactile stimuli with the sensory memory trace, was larger in the tactile attentional task. Additionally, the sMMN latency increased with the increasing visual attentional load, which indicates a decay of tactile sensory memory. Our results indicate that the more attention resources are allocated for a tactile sensation, the more favorable it is to the generation of tactile sensory memory.
Age-Related Deficits in Binocular Vision Are Associated With Poorer Inhibitory Control in Healthy Older Adults
Authors: Lin Grace , Al Ani Raghda , Niechwiej-Szwedo Ewa
Published: November 2020

A robust association between reduced visual acuity and cognitive function in older adults has been revealed in large population studies. The aim of this work was to assess the relation between stereoacuity, a key aspect of binocular vision, and inhibitory control, an important component of executive functions. Inhibition was tested using the antisaccade task in older adults with normal or reduced stereopsis (study 1), and in young adults with transiently reduced stereopsis (study 2). Older adults with reduced stereopsis made significantly more errors on the antisaccade task in comparison to those with normal stereopsis. Specifically, there was a significant correlation between stereoacuity and antisaccade errors (r = 0.27, p = 0.019). In contrast, there were no significant differences in antisaccade errors between the normal and reduced stereopsis conditions in the young group. Altogether, results suggest that the association between poorer stereopsis and lower inhibitory control in older adults might arise due to central nervous system impairment that affects the processing of binocular disparity and antisaccades. These results add to a growing body of literature, which highlights the interdependence of sensory and cognitive decline in older adults.
Structure-Function of Neuronal Nicotinic Acetylcholine Receptor Inhibitors Derived From Natural Toxins
Authors: Ho Thao N. T. , Abraham Nikita , Lewis Richard J.
Published: November 2020

Neuronal nicotinic acetylcholine receptors (nAChRs) are prototypical cation-selective, ligand-gated ion channels that mediate fast neurotransmission in the central and peripheral nervous systems. nAChRs are involved in a range of physiological and pathological functions and hence are important therapeutic targets. Their subunit homology and diverse pentameric assembly contribute to their challenging pharmacology and limit their drug development potential. Toxins produced by an extensive range of algae, plants and animals target nAChRs, with many proving pivotal in elucidating receptor pharmacology and biochemistry, as well as providing templates for structure-based drug design. The crystal structures of these toxins with diverse chemical profiles in complex with acetylcholine binding protein (AChBP), a soluble homolog of the extracellular ligand-binding domain of the nAChRs and more recently the extracellular domain of human α9 nAChRs, have been reported. These studies have shed light on the diverse molecular mechanisms of ligand-binding at neuronal nAChR subtypes and uncovered critical insights useful for rational drug design. This review provides a comprehensive overview and perspectives obtained from structure and function studies of diverse plant and animal toxins and their associated inhibitory mechanisms at neuronal nAChRs.
Challenges Associated With the Design and Deployment of Food Intake Urine Biomarker Technology for Assessment of Habitual Diet in Free-Living Individuals and Populations—A Perspective
Authors: Beckmann Manfred , Wilson Thomas , Lloyd Amanda J. , Torres Duarte , Goios Ana , Willis Naomi D. , Lyons Laura , Phillips Helen , Mathers John C. , Draper John
Published: November 2020

Improvement of diet at the population level is a cornerstone of national and international strategies for reducing chronic disease burden. A critical challenge in generating robust data on habitual dietary intake is accurate exposure assessment. Self-reporting instruments (e.g., food frequency questionnaires, dietary recall) are subject to reporting bias and serving size perceptions, while weighed dietary assessments are unfeasible in large-scale studies. However, secondary metabolites derived from individual foods/food groups and present in urine provide an opportunity to develop potential biomarkers of food intake (BFIs). Habitual dietary intake assessment in population surveys using biomarkers presents several challenges, including the need to develop affordable biofluid collection methods, acceptable to participants that allow collection of informative samples. Monitoring diet comprehensively using biomarkers requires analytical methods to quantify the structurally diverse mixture of target biomarkers, at a range of concentrations within urine. The present article provides a perspective on the challenges associated with the development of urine biomarker technology for monitoring diet exposure in free-living individuals with a view to its future deployment in “real world” situations. An observational study (n = 95), as part of a national survey on eating habits, provided an opportunity to explore biomarker measurement in a free-living population. In a second food intervention study (n = 15), individuals consumed a wide range of foods as a series of menus designed specifically to achieve exposure reflecting a diversity of foods commonly consumed in the UK, emulating normal eating patterns. First Morning Void urines were shown to be suitable samples for biomarker measurement. Triple quadrupole mass spectrometry, coupled with liquid chromatography, was used to assess simultaneously the behavior of a panel of 54 potential BFIs. This panel of chemically diverse biomarkers, reporting intake of a wide range of commonly-consumed foods, can be extended successfully as new biomarker leads are discovered. Towards validation, we demonstrate excellent discrimination of eating patterns and quantitative relationships between biomarker concentrations in urine and the intake of several foods. In conclusion, we believe that the integration of information from BFI technology and dietary self-reporting tools will expedite research on the complex interactions between dietary choices and health.
The Effect of Solar-Wind Turbulence on Magnetospheric Activity
Authors: D’Amicis R. , Telloni D. , Bruno R.
Published: November 2020

The solar wind is a highly turbulent medium exhibiting scalings of the fluctuations ranging over several decades of scales from the correlation length down to proton and electron gyroradii, thus suggesting a self-similar nature for these fluctuations. During its journey, the solar wind encounters the region of space surrounding Earth dominated by the geomagnetic field which is called magnetosphere. The latter is exposed to the continuous buffeting of the solar wind which determines its characteristic comet-like shape. The solar wind and the magnetosphere interact continously, thus constituting a coupled system, since perturbations in the interplanetary medium cause geomagnetic disturbances. However, strong variations in the geomagnetic field occur even in absence of large solar perturbations. In this case, a major role is attributed to solar wind turbulence as a driver of geomagnetic activity especially at high latitudes. In this review, we report about the state-of-art related to this topic. Since the solar wind and the magnetosphere are both high Reynolds number plasmas, both follow a scale-invariant dynamics and are in a state far from equilibrium. Moreover, the geomagnetic response, although closely related to the changes of the interplanetary magnetic field condition, is also strongly affected by the intrinsic dynamics of the magnetosphere generated by geomagnetic field variations caused by the internal conditions.
Stereo Perfect Metamaterial Absorber Based on Standing Gear-Shaped Resonant Structure With Wide-Incident-Angle Stability
Authors: Deng Guangsheng , Lv Kun , Sun Hanxiao , Yin Zhiping , Yang Jun
Published: November 2020

In this work, a single-band metamaterial absorber (MA) based on a three dimensional (3D) resonant structure is presented. The unit cell is composed of a standing gear-shaped resonator, which is embedded in the dielectric substrate. The proposed 3D MA is ultrathin with a total thickness of 2.3 mm, corresponding 0.077λ0 at its center frequency. The simulation results demonstrate a high absorption peak at 10.1 GHz with absorptivity of 99.9%. The proposed 3D MA is insensitive to the polarization of the incident wave due to its rotationally symmetric structure. Moreover, the proposed 3D MA exhibits a wide-incident-angle stability, as absorptivity of more than 85% can be achieved for both TE and TM incidences with incident angle up to 60°. Most importantly, multiband electromagnetic wave absorption of the stereo MA can be enabled by adjusting the structural parameters of the standing gear. The proposed structure is compatible with 3D printing technology and has potential applications in electromagnetic shielding.
Defective Titanium Dioxide-supported Ultrasmall Au Clusters for Photocatalytic Hydrogen Production
Authors: Zhang Xiaoqin , Zhang Wenna , Xu Yuanmei , Jin Mingliang
Published: November 2020

Ultrasmall precious metal clusters have attracted extensive attention for providing a very specific surface and promoting electron transfer. In this work, ultrasmall Au clusters based on defective TiO2 nanosheets (Au/D-TiO2) were prepared and introduced into photocatalytic hydrogen evolution. Different defects of TiO2 nanosheets (D-TiO2) were constructed using a heating process and then loaded with Au clusters. Compared with bare TiO2, Au clusters established on defective TiO2 nanosheets with a narrower band gap showed higher light absorption performances, resulting in obviously enhanced photocatalytic hydrogen production performances. The Au/D-TiO2 displayed the greatly enhanced photocatalytic hydrogen evolution activity of 3,142.33 μmol h−1 g−1, which was over 45 times than the pure TiO2. The results showed that the catalysts had good prospects in the field of photocatalytic hydrogen production.
Mathematical Modeling for the Physiological and Clinical Investigation of Glucose Homeostasis and Diabetes
Authors: Mari Andrea , Tura Andrea , Grespan Eleonora , Bizzotto Roberto
Published: November 2020

Mathematical modeling in the field of glucose metabolism has a longstanding tradition. The use of models is motivated by several reasons. Models have been used for calculating parameters of physiological interest from experimental data indirectly, to provide an unambiguous quantitative representation of pathophysiological mechanisms, to determine indices of clinical usefulness from simple experimental tests. With the growing societal impact of type 2 diabetes, which involves the disturbance of the glucose homeostasis system, development and use of models in this area have increased. Following the approaches of physiological and clinical investigation, the focus of the models has spanned from representations of whole body processes to those of cells, i.e., from in vivo to in vitro research. Model-based approaches for linking in vivo to in vitro research have been proposed, as well as multiscale models merging the two areas. The success and impact of models has been variable. Two kinds of models have received remarkable interest: those widely used in clinical applications, e.g., for the assessment of insulin sensitivity and β-cell function and some models representing specific aspects of the glucose homeostasis system, which have become iconic for their efficacy in describing clearly and compactly key physiological processes, such as insulin secretion from the pancreatic β cells. Models are inevitably simplified and approximate representations of a physiological system. Key to their success is an appropriate balance between adherence to reality, comprehensibility, interpretative value and practical usefulness. This has been achieved with a variety of approaches. Although many models concerning the glucose homeostasis system have been proposed, research in this area still needs to address numerous issues and tackle new opportunities. The mathematical representation of the glucose homeostasis processes is only partial, also because some mechanisms are still only partially understood. For in vitro research, mathematical models still need to develop their potential. This review illustrates the problems, approaches and contribution of mathematical modeling to the physiological and clinical investigation of glucose homeostasis and diabetes, focusing on the most relevant and stimulating models.
Immunohistochemical Expression of the Alpha Nicotinic Acetylcholine Receptor 7 in the Human Normal, Diabetic, and Preeclamptic Placenta and Products of Conception
Authors: Alwazzan Ahmad , Mehboob Riffat , Gilani Syed Amir , Hassan Amber , Perveen Shahida , Tanvir Imrana , Waseem Humaira , Ehsan Kashifa , Ahmad Fridoon Jawad , Akram Javed
Published: November 2020

Preeclampsia (PE) and gestational diabetes (GD) are complications in advanced pregnancy while miscarriage for early pregnancy. However, the etiological factors are not well understood. Smoking has been associated with these complications as well as the sudden intrauterine deaths, sudden infant death, miscarriages, and still births. However, the immunolocalization of alpha 7 nicotine acetylcholine receptor (α7-nAChR) is not studied.

Materials and Methods: α7-nAChR subunit expression was evaluated in 10 paraffin-embedded placental tissues after delivery and 10 tissue samples of products of conception during first trimester by immunohistochemistry. Among the placental tissues, two samples were normal placental tissue, four from PE mother, and four from GD mother. The expression of α7-nAChR was compared between the two groups in general and within the subgroups of placenta as well. Protein expression was evaluated using the nuclear labeling index (%) of villi with positive cells stained, positive cells in the decidua, and intensity of staining in the outer villous trophoblast layer.

Results: The expression of α7-nAChR protein was high in all the cases of placenta and products of conception (POCs). α7-nAChR expression showed no notable differences among different cases of miscarriages irrespective of the mother’s age and gestational age at which the event occurred. However, there were some changes among the normal, PE, and GD placental groups in the linings of the blood vessels. Changes were restricted to the villi (as opposed to the decidua) lining cells, both cytotrophoblast and syncytiotrophoblast, and were specific to the α7 subunit. PE blood vessel lining was thicker and showed more expression of this receptor in endothelial cells and myofibroblasts in PE and GD groups. In POCs, the strong expression was observed in the decidua myocytes of maternal blood vessels and in syncytiotrophoblast and cytotrophoblast of chronic villi.

Conclusion: Nicotine acetyl choline receptors are found to be expressed highly in the placental tissues and in products of conception. They may be associated with the sudden perinatal deaths and miscarriages or complications of pregnancy.
A Comprehensive and Comparative Analysis of the Fucoidan Compositional Data Across the Phaeophyceae
Authors: Ponce Nora M. A. , Stortz Carlos A.
Published: November 2020

In the current review, compositional data on fucoidans extracted from more than hundred different species were surveyed through the available literature. The analysis of crude extracts, purified extracts or carefully isolated fractions is included in tabular form, discriminating the seaweed source by its taxonomical order (and sometimes the family). This survey was able to encounter some similarities between the different species, as well as some differences. Fractions which were obtained through anion-exchange chromatography or cationic detergent precipitation showed the best separation patterns: the fractions with low charge correspond mostly to highly heterogeneous fucoidans, containing (besides fucose) other monosaccharides like xylose, galactose, mannose, rhamnose, and glucuronic acid, and contain low-sulfate/high uronic acid proportions, whereas those with higher total charge usually contain mainly fucose, accompanied with variable proportions of galactose, are highly sulfated and show almost no uronic acids. The latter fractions are usually the most biologically active. Fractions containing intermediate proportions of both polysaccharides appear at middle ionic strengths. This pattern is common for all the orders of brown seaweeds, and most differences appear from the seaweed source (habitat, season), and from the diverse extraction, purification, and analytitcal methods. The Dictyotales appear to be the most atypical order, as usually large proportions of mannose and uronic acids appear, and thus they obscure the differences between the fractions with different charge. Within the family Alariaceae (order Laminariales), the presence of sulfated galactofucans with high galactose content (almost equal to that of fucose) is especially noteworthy.
Late Frontal Negativity Discriminates Outcomes and Intentions in Trust-Repayment Behavior
Authors: Aspé-Sánchez Mauricio , Mengotti Paola , Rumiati Raffaella , Rodríguez-Sickert Carlos , Ewer John , Billeke Pablo
Published: November 2020

Altruism (a costly action that benefits others) and reciprocity (the repayment of acts in kind) differ in that the former expresses preferences about the outcome of a social interaction, whereas the latter requires, in addition, ascribing intentions to others. Interestingly, an individual’s behavior and neurophysiological activity under outcome- versus intention-based interactions has not been compared directly using different endowments in the same subject and during the same session. Here, we used a mixed version of the Dictator and the Investment games, together with electroencephalography, to uncover a subject’s behavior and brain activity when challenged with endowments of different sizes in contexts that call for an altruistic (outcome-based) versus a reciprocal (intention-based) response. We found that subjects displayed positive or negative reciprocity (reciprocal responses greater or smaller than that for altruism, respectively) depending on the amount of trust they received. Furthermore, a subject’s late frontal negativity differed between conditions, predicting responses to trust in intentions-based trials. Finally, brain regions related with mentalizing and cognitive control were the cortical sources of this activity. Thus, our work disentangles the behavioral components present in the repayment of trust, and sheds light on the neural activity underlying the integration of outcomes and perceived intentions in human economic interactions.
Demonstratives in Spatial Language and Social Interaction: An Interdisciplinary Review
Authors: Diessel Holger , Coventry Kenny R.
Published: November 2020

This paper offers a review of research on demonstratives from an interdisciplinary perspective. In particular, we consider the role of demonstratives in current research on language universals, language evolution, language acquisition, multimodal communication, signed language, language and perception, language in interaction, spatial imagery, and discourse processing. Traditionally, demonstratives are analyzed as a particular class of spatial deictics. Yet, a number of recent studies have argued that space is largely irrelevant to deixis and that demonstratives are primarily used for social and interactive purposes. Synthesizing findings in the literature, we conclude that demonstratives are a very special class of linguistic items that are foundational to both spatial and social aspects of language and cognition.
The Diminishing Effect of Transformational Leadership on the Relationship Between Task Characteristics, Perceived Meaningfulness, and Work Engagement
Authors: Meng Fanxing , Wang Yi , Xu Wenying , Ye Junhui , Peng Lin , Gao Peng
Published: November 2020

The topic of employee work engagement in the public sector has attracted broad attention because it is critical to the efficiency and effectiveness of public services. Based on the Job Characteristics Model (JCM) and the Integrative Theory of Employee Engagement (ITEE), the present research adopts a multilevel design to examine a moderated mediation model in which task characteristics (i.e., task autonomy and task significance as level-1 predictors) and social context (i.e., transformational leadership as a level-2 moderator) jointly impact employee work engagement via individual perception of meaningfulness in work. A total of 349 grassroots police officers from 35 police substations were invited to anonymously complete a survey via mobile app. After performing the cross-sectional analysis, the results indicated that in contrast to task significance, the conditional effect of task autonomy on work engagement via perceived meaningfulness was more positive at a lower level of transformational leadership. Implications, limitations, and future research directions are discussed.
Talent Research in Sport 1990–2018: A Scoping Review
Authors: Baker Joseph , Wilson Stuart , Johnston Kathryn , Dehghansai Nima , Koenigsberg Aaron , de Vegt Steven , Wattie Nick
Published: November 2020

Several recent systematic and targeted reviews have highlighted limitations in our understanding of talent in sport. However, a comprehensive profile of where the scientific research has focused would help identify gaps in current knowledge. Our goal in this scoping review was (a) to better understand what others have done in the field of research (e.g., what groups have been examined using what research designs and in what areas), (b) to summarize the constituent areas of research in a meaningful way, (c) to help identify gaps in the research, and (d) to encourage future research to address these gaps. Peer-reviewed articles written in English that met several inclusion criteria were analyzed. A total of 1,899 articles were identified, and the descriptive findings revealed a relatively narrow focus of research on talent in sport. Specifically, the majority of examined articles focused on (a) males only, (b) the sport of soccer, (c) perceptual cognitive variables, (d) developing athletes, (e) adult samples, and (f) cross-sectional designs. For better or worse, the concept of talent remains a central element of how coaches, practitioners, and scientists think about athlete development. Findings from this scoping review highlight the continued need to explore issues related to talent identification, selection, and development in more diverse samples (e.g., female athletes and younger ages) and contexts (e.g., from Africa, Asia, and South America). There is also a clear necessity to focus on under-researched areas using alternative methodologies.
What Is Whooping Cough and How Can We Protect Ourselves?
Authors: Guiso Nicole
Published: November 2020

Whooping cough is a very contagious respiratory disease, caused by a bacterium called Bordetella pertussis. Babies <3 months of age can die from whooping cough, and it is also particularly dangerous for seniors. Children are protected from whooping cough if they have the tools to eliminate the bacterium when they come in contact with it. These tools are cells and antibodies in the blood, and this protection can be acquired by vaccination. There are two types of vaccines against whooping cough: one contains whole bacteria that have been inactivated by heat or chemicals, and another contains inactivated factors removed from the bacteria. After vaccination with either vaccine, children are protected against the bacterium. However, after several years, the protective cells and antibodies decrease or disappear, and it is important for children to get revaccinated. These revaccinations are called vaccine boosters, and they are recommended for children, adolescents, adults, seniors, and pregnant women.
Influence of Line Strategy Between Two Turns on Performance in Giant Slalom
Authors: Delhaye Clément , Cross Matthew R. , Bowen Maximilien , Samozino Pierre , Hintzy Frédérique
Published: November 2020

In alpine ski racing, different line choices can drastically affect turn or sectional performance. The straight-line transition between two turns is the main phase where skiers can gain speed in a race, open their trajectory, or reduce their path length. Between two turns, a skier can foster speed increase by spending more time in a straight line, inducing sharper turning phases (Z strategy). Inversely, speed can be conserved during the entire turn cycle by performing long curved turns separated by a short straight line (S strategy). This research aimed to evaluate the kinetic and kinematic specificities associated with the line strategy and to explore interactions of selected strategy with skier performance and energy dissipation. A mixed-level population of male alpine skiers (n = 17) skied a timed giant-slalom course while equipped with specialized force plates and a positional device collecting synchronized normal ground reaction force and position-time data, respectively. Time of edge switch was computed from the force signal as the period with the lowest force application on the outside ski. From positional data, turn cycles were separated into turning and straight-line phases (radius bellow and above 30 m, respectively). Time length, path length in the straight line, speed amplitude, and change in specific mechanical energy were computed for each turn and averaged for each skier. The path length during straight line was used to continuously characterize the line strategy within the spectrum between the Z (long straight line) and S (short straight line) strategy. Path length in the straight line was correlated with the amplitude of speed over a straight line (r = 0.672, p = 0.003) and relative and absolute time spent in the straight line (r = 0.967, p < 0.001). However, path length in straight line was not correlated with decrease of speed in the following turn (r = −0.418, p = 0.390) or time without force application on the outside ski (r = 0.195, p = 0.453). While higher-performing athletes on the course performed turns during which they dissipated less energy when normalized to entry speed (r = −0.620, p = 0.008), it appears they did so with variable turn strategies approaches.
Thermoregulatory and Behavioral Responses of Buffaloes With and Without Direct Sun Exposure During Abnormal Environmental Condition in Marajó Island, Pará, Brazil
Authors: Athaíde Letícia Godinho , Joset Waleria Cristina Lopes , de Almeida Jean Caio Figueiredo , Pantoja Messy Hennear de Andrade , Noronha Rafaella de Paula Pacheco , Bezerra Andréia Santana , Barbosa Antônio Vinicius Corrêa , Martorano Lucieta Guerreiro , da Silva Jamile Andréa Rodrigues , Lourenço Júnior José de Brito
Published: November 2020

This study aimed to assess the effect of thermal-hydraulic variables in female buffaloes with or without direct solar exposure in a year of strong El Niño through behavior responses and infrared thermography to reinforce the environmental comfort indicators, in Marajó Island, Pará, Brazil. The experiment was carried out in Cachoeira do Arari municipality and 20 female Murrah buffaloes were randomly assigned to two groups: Group WS (n= 10) was kept in pickets with native trees. Group NS (n= 10) was kept in crush squeeze with no shade. Data on air temperature (AT, °C), relative air humidity (RH, %), wind velocity (WV, m/s), rectal temperature (RT), respiratory rate (RR), and body surface temperature (BST) were collected. Practical Buffalo Comfort Climatic Condition Index (BCCCI), practical Buffalo Environmental Comfort Index (BECI), Temperature and Humidity Index (THI) and Benezra's Thermal Comfort Index (BTCI) were obtained. Infrared thermography analysis was carried out with a FLIR T-series T640bx camera. Data on time spent grazing, ruminating, idleness, and in other activities were recorded. A significant difference in AT of ~1°C was found between the groups at 6 a.m., 10 a.m. and 6 p.m. THI indicated emergency conditions. Female buffaloes were at danger PBCCCI conditions at 2 p.m. There was also significant difference for RT between treatments at 10 a.m., 2 p.m. and 6 p.m., whose values were higher (P< 0.05) for animals from NS Group, with the highest mean time at 2 p.m. Pearson correlation was significant and positive (P< 0.01) between RT mean and VUL, TI and ORB mean, maximum and minimum temperatures. The total time given to grazing was 518.2 min for the group NS and 629.5 min for the group WS. Rumination was more pronounced in the afternoon shift for the group NS. Buffaloes kept in a system with trees graze, ruminate and perform other activities with more intensity than animals raised in systems without access to shade, and tend to hyperthermia, mainly at 10 a.m. and 2 p.m., in Marajó Island, Pará, Brazil.
Local Small Ruminant Grazing in the Monti Foy Area (Italy): The Relationship Between Grassland Biodiversity Maintenance and Added-Value Dairy Products
Authors: Claps Salvatore , Mecca Marisabel , Di Trana Adriana , Sepe Lucia
Published: November 2020

The literature indicates that grazing small ruminants, when adequately managed, contributes to grassland biodiversity maintenance. On the other hand, milk and cheese from grazing animals show higher nutritional and aromatic quality than those from stall-fed animals. The relationship between the two issues has rarely been addressed. This article provides information for a discussion of this relationship. First, two case studies are reported. Local breeds of small ruminants fed by grazing on pastures within the Special Area of Conservation “Monti Foy” in the Northwestern Basilicata region (Italy), with a stocking rate of 4.0 LU ha−1 year−1, showed the best effectiveness for the maintenance of grassland botanical biodiversity. Milk and cheese from pasture-fed goats showed higher contents of beneficial fatty acids, phenols, and vitamins A and E; higher degree of antioxidant protection; and richer volatile compound profiles, in particular for terpenes content. Finally, some recommendations for the management of grazing systems in similar mountain areas are offered, including a viable approach for land managers to preserve the grassland biodiversity of pastures and provide high-quality products that are valuable both for their nutritional quality and for their contribution to the economic sustainability of mountain communities.
Broiler Farms and Carcasses Are an Important Reservoir of Multi-Drug Resistant Escherichia coli in Ecuador
Authors: Ortega-Paredes David , de Janon Sofía , Villavicencio Fernando , Ruales Katherine Jaramillo , De La Torre Kenny , Villacís José E. , Wagenaar Jaap A. , Matheu Jorge , Bravo-Vallejo Camila , Fernández-Moreira Esteban , Vinueza-Burgos Christian
Published: November 2020

Antimicrobial resistance (AMR) is a major health threat for public and animal health in the twenty-first century. In Ecuador, antibiotics have been used by the poultry industry for decades resulting in the presence of multi-drug resistant (MDR) bacteria in the poultry meat production chain, with the consequent risk for public health. This study evaluated the prevalence of ESBL/AmpC and mcr genes in third-generation cephalosporin-resistant Escherichia coli (3GC-R E. coli) isolated from broiler farms (animal component), broiler carcasses (food component), and human enteritis (human component) in Quito-Ecuador. Samples were collected weekly from November 2017 to November 2018. For the animal, food, and human components, 133, 335, and 302 samples were analyzed, respectively. Profiles of antimicrobial resistance were analyzed by an automated microdilution system. Resistance genes were studied by PCR and Sanger sequencing. From all samples, 122 (91.7%), 258 (77%), and 146 (48.3%) samples were positive for 3GC-R E. coli in the animal, food, and human components, respectively. Most of the isolates (472/526, 89.7%) presented MDR phenotypes. The ESBL blaCTX-M-55, blaCTX-M-3, blaCTX-M-15, blaCTX-M-65, blaCTX-M-27, and blaCTX-M-14 were the most prevalent ESBL genes while blaCMY-2 was the only AmpC detected gene. The mcr-1 gene was found in 20 (16.4%), 26 (10.1%), and 3 (2.1%) of isolates from animal, food, and human components, respectively. The implication of poultry products in the prevalence of ESBL/AmpC and mcr genes in 3GC-R must be considered in the surveillance of antimicrobial resistance.