bluuebunny/crossref_metadata_2025 huggingface dataset:
Supplementary Table 10 from Associations between Physical Activity and Incidence of Cancer among Overweight Adults in Korea: Results from the Health Examinees-G Study
Authors: Choi Jaesung , Park JooYong , Kim Ji-Eun , Lee Miyoung , Kang Daehee , Shin Aesun , Choi Ji-Yeob
Published: June 2023

Supplementary Table 10 shows the association of physical activity with gastric cancer incidence stratified by obesity status
Supplementary Table 1 from Associations between Physical Activity and Incidence of Cancer among Overweight Adults in Korea: Results from the Health Examinees-G Study
Authors: Choi Jaesung , Park JooYong , Kim Ji-Eun , Lee Miyoung , Kang Daehee , Shin Aesun , Choi Ji-Yeob
Published: June 2023

Supplementary Table 1 shows the categorization of physical activity
Supplementary Table S4 from Mitophagy Promotes Resistance to BH3 Mimetics in Acute Myeloid Leukemia
Authors: Glytsou Christina , Chen Xufeng , Zacharioudakis Emmanouil , Al-Santli Wafa , Zhou Hua , Nadorp Bettina , Lee Soobeom , Lasry Audrey , Sun Zhengxi , Papaioannou Dimitrios , Cammer Michael , Wang Kun , Zal Tomasz , Zal Malgorzata Anna , Carter Bing Z. , Ishizawa Jo , Tibes Raoul , Tsirigos Aristotelis , Andreeff Michael , Gavathiotis Evripidis , Aifantis Iannis
Published: June 2023

Supplementary Table S4 shows autophagy related genes and their ranking in NMF analysis of scRNAseq data.
Supplementary Table S2 from Mitophagy Promotes Resistance to BH3 Mimetics in Acute Myeloid Leukemia
Authors: Glytsou Christina , Chen Xufeng , Zacharioudakis Emmanouil , Al-Santli Wafa , Zhou Hua , Nadorp Bettina , Lee Soobeom , Lasry Audrey , Sun Zhengxi , Papaioannou Dimitrios , Cammer Michael , Wang Kun , Zal Tomasz , Zal Malgorzata Anna , Carter Bing Z. , Ishizawa Jo , Tibes Raoul , Tsirigos Aristotelis , Andreeff Michael , Gavathiotis Evripidis , Aifantis Iannis
Published: June 2023

Supplementary Table S2 shows the correlation score of MFN2 expression with the ex vivo drug response (IC50, BeatAML data).
Supplementary Table S1 from Mitophagy Promotes Resistance to BH3 Mimetics in Acute Myeloid Leukemia
Authors: Glytsou Christina , Chen Xufeng , Zacharioudakis Emmanouil , Al-Santli Wafa , Zhou Hua , Nadorp Bettina , Lee Soobeom , Lasry Audrey , Sun Zhengxi , Papaioannou Dimitrios , Cammer Michael , Wang Kun , Zal Tomasz , Zal Malgorzata Anna , Carter Bing Z. , Ishizawa Jo , Tibes Raoul , Tsirigos Aristotelis , Andreeff Michael , Gavathiotis Evripidis , Aifantis Iannis
Published: June 2023

Supplementary Table 1 shows the results of our CRISPRi screens.
Supplementary Figures 1-7 from Mitophagy Promotes Resistance to BH3 Mimetics in Acute Myeloid Leukemia
Authors: Glytsou Christina , Chen Xufeng , Zacharioudakis Emmanouil , Al-Santli Wafa , Zhou Hua , Nadorp Bettina , Lee Soobeom , Lasry Audrey , Sun Zhengxi , Papaioannou Dimitrios , Cammer Michael , Wang Kun , Zal Tomasz , Zal Malgorzata Anna , Carter Bing Z. , Ishizawa Jo , Tibes Raoul , Tsirigos Aristotelis , Andreeff Michael , Gavathiotis Evripidis , Aifantis Iannis
Published: June 2023

Supplemental Figure S1 shows the integration of CRISPR/Cas9 loss-of-function screens to identify dependencies and liabilities in BH3-mimetics treatments.
Supplemental Figure S2 demonstrates that increased mitochondria-ER interactions contribute to BH3-mimetics resistance.
Supplemental Figure S3 shows that mitophagy affects the responsiveness of AML cells to BH3-mimetics.
Supplemental Figure S4 reveals that enhanced autophagic clearance of mitochondria as a mechanism of resistance to BH3-mimetics in AML.
Supplemental Figure S5 exhibits the synergism between BH3-mimetics and macroautophagy inhibition in human AML.
Supplemental Figure S6 shows that deletion of MFN2 or MARCH5 sensitizes AML cells to BH3-mimetics.
Supplemental Figure S7 displays that targeting of MFN2 or MARCH5 impairs the process of mitophagy.
Supplementary Table S4 from The Polyamine–Hypusine Circuit Controls an Oncogenic Translational Program Essential for Malignant Conversion in MYC-Driven Lymphoma
Authors: Nakanishi Shima , Li Jiannong , Berglund Anders E. , Kim Youngchul , Zhang Yonghong , Zhang Ling , Yang Chunying , Song Jinming , Mirmira Raghavendra G. , Cleveland John L.
Published: June 2023

Supplementary Table S4 lists oncogenes and tumor suppressor genes analyzed for changes in translation efficiency (TE) following depletion of eIF5A or DHPS, related to Fig. 5E.
Supplementary Table S3 from The Polyamine–Hypusine Circuit Controls an Oncogenic Translational Program Essential for Malignant Conversion in MYC-Driven Lymphoma
Authors: Nakanishi Shima , Li Jiannong , Berglund Anders E. , Kim Youngchul , Zhang Yonghong , Zhang Ling , Yang Chunying , Song Jinming , Mirmira Raghavendra G. , Cleveland John L.
Published: June 2023

Supplementary Table S3 lists 665 shared differentially translated transcripts (DTT) identified following depletion of eIF5A or DHPS, related to Fig. 5C.
Video 1 from Mitochondria Transfer from Mesenchymal Stem Cells Confers Chemoresistance to Glioblastoma Stem Cells through Metabolic Rewiring
Authors: Nakhle Jean , Khattar Khattar , Özkan Tülin , Boughlita Adel , Abba Moussa Daouda , Darlix Amélie , Lorcy Frédérique , Rigau Valérie , Bauchet Luc , Gerbal-Chaloin Sabine , Daujat-Chavanieu Martine , Bellvert Floriant , Turchi Laurent , Virolle Thierry , Hugnot Jean-Philippe , Buisine Nicolas , Galloni Mireille , Dardalhon Valérie , Rodriguez Anne-Marie , Vignais Marie-Luce
Published: June 2023

Cocultures of red MitoTracker-labeled MSCs and green CellTracker-labeled GSCs
Supplementary Materials and Methods from Mitochondria Transfer from Mesenchymal Stem Cells Confers Chemoresistance to Glioblastoma Stem Cells through Metabolic Rewiring
Authors: Nakhle Jean , Khattar Khattar , Özkan Tülin , Boughlita Adel , Abba Moussa Daouda , Darlix Amélie , Lorcy Frédérique , Rigau Valérie , Bauchet Luc , Gerbal-Chaloin Sabine , Daujat-Chavanieu Martine , Bellvert Floriant , Turchi Laurent , Virolle Thierry , Hugnot Jean-Philippe , Buisine Nicolas , Galloni Mireille , Dardalhon Valérie , Rodriguez Anne-Marie , Vignais Marie-Luce
Published: June 2023

TMZ dose-response assay

Caspase-GloⓇ 3/7 assay

Cytotox assay

Extracellular Flux Assays

Cell Proliferation

Western Blot

Flow Cytometry

Metabolite usage Mitoplates

Mass Spectrometry Quantification

13C stable isotope tracing experiments

Preparation and quantification of MSC mtDNA in GSCs

Imaging
Figure S5 from Mitochondria Transfer from Mesenchymal Stem Cells Confers Chemoresistance to Glioblastoma Stem Cells through Metabolic Rewiring
Authors: Nakhle Jean , Khattar Khattar , Özkan Tülin , Boughlita Adel , Abba Moussa Daouda , Darlix Amélie , Lorcy Frédérique , Rigau Valérie , Bauchet Luc , Gerbal-Chaloin Sabine , Daujat-Chavanieu Martine , Bellvert Floriant , Turchi Laurent , Virolle Thierry , Hugnot Jean-Philippe , Buisine Nicolas , Galloni Mireille , Dardalhon Valérie , Rodriguez Anne-Marie , Vignais Marie-Luce
Published: June 2023

MSC mitochondria modify the usage of metabolites by GCSs in response to TMZ CTRL Mito TMZ CTRL Mito Mito TMZ (A) Metabolic substrate consumption of GSCs expressed as metabolite initial consumption rates (Biolog MitoPlates). Mean values ± SEM (4 independent experiments). (B) Production of cis-aconitate, succinate and L-malate expressed as a percentage of all TCA metabolites produced in each experimental condition. Tukey boxplots with one-way ANOVA, *p < 0.05, ***p < 0.001.
Figure S2 from Mitochondria Transfer from Mesenchymal Stem Cells Confers Chemoresistance to Glioblastoma Stem Cells through Metabolic Rewiring
Authors: Nakhle Jean , Khattar Khattar , Özkan Tülin , Boughlita Adel , Abba Moussa Daouda , Darlix Amélie , Lorcy Frédérique , Rigau Valérie , Bauchet Luc , Gerbal-Chaloin Sabine , Daujat-Chavanieu Martine , Bellvert Floriant , Turchi Laurent , Virolle Thierry , Hugnot Jean-Philippe , Buisine Nicolas , Galloni Mireille , Dardalhon Valérie , Rodriguez Anne-Marie , Vignais Marie-Luce
Published: June 2023

Quantification of mitochondrial DNA (mtDNA) following transfer of MSC mitochondria to GSCs (A) Detection of MSC mtDNA in GSCs. MSC mtDNA concentrations are expressed relative to GSC mtDNA. Mean with SEM, t test with Welch correction, *p < 0.05. (B) Total mtDNA concentrations in GSCs at different time points after MSC mitochondria transfer, expressed relative to GSC genomic DNA. Mean + SEM and multiple t tests, *p < 0.05.
FIGURE 6 from Mitochondria Transfer from Mesenchymal Stem Cells Confers Chemoresistance to Glioblastoma Stem Cells through Metabolic Rewiring
Authors: Nakhle Jean , Khattar Khattar , Özkan Tülin , Boughlita Adel , Abba Moussa Daouda , Darlix Amélie , Lorcy Frédérique , Rigau Valérie , Bauchet Luc , Gerbal-Chaloin Sabine , Daujat-Chavanieu Martine , Bellvert Floriant , Turchi Laurent , Virolle Thierry , Hugnot Jean-Philippe , Buisine Nicolas , Galloni Mireille , Dardalhon Valérie , Rodriguez Anne-Marie , Vignais Marie-Luce
Published: June 2023

MSC mitochondria induce a higher orotate turnover in GSCs which supports resistance to TMZ. A and B, [U-13C]-glucose and [U-13C]-glutamine orotate isotope profiling in GSCs with and/or without MSC mitochondria and treated or not with TMZ (48 hours; n = 3). Mean + SEM. Statistical analysis by Student t test. ***, P < 0.001. C, Schematic of BRQ inhibition of DHODH-dependent orotate production (BioRender). D, Effect of BRQ (100 nmol/L) on the survival of GSCs (GB4 and GB5, from 2 donors) to TMZ treatment (50 μmol/L) following acquisition of MSC mitochondria (3 MSC donors for each GSC). Two-way ANOVA, *, P < 0.05; **, P < 0.01; ***, P < 0.001; n = 4, each dot represents a culture well.
FIGURE 5 from Mitochondria Transfer from Mesenchymal Stem Cells Confers Chemoresistance to Glioblastoma Stem Cells through Metabolic Rewiring
Authors: Nakhle Jean , Khattar Khattar , Özkan Tülin , Boughlita Adel , Abba Moussa Daouda , Darlix Amélie , Lorcy Frédérique , Rigau Valérie , Bauchet Luc , Gerbal-Chaloin Sabine , Daujat-Chavanieu Martine , Bellvert Floriant , Turchi Laurent , Virolle Thierry , Hugnot Jean-Philippe , Buisine Nicolas , Galloni Mireille , Dardalhon Valérie , Rodriguez Anne-Marie , Vignais Marie-Luce
Published: June 2023

MSC mitochondria modify metabolite fluxes in GSCs. [U-13C]-glucose and [U-13C]-glutamine isotope profiling of GSCs with and/or without MSC mitochondria and treated or not with TMZ (48 hours; n = 3). A, Glycolysis intermediates. B, Isotopologues of TCA cycle metabolites from 13C-glucose (24 and 48 hours). C, Isotopologues of TCA cycle metabolites from 13C-glutamine (48 hours). M3/M4 and M5/M3 ratios of isotopologues in GSCs with/out MSC mitochondria. Mean + SEM. Two-tailed unpaired t tests; ***, P < 0.001. Colors refer to the number of carbon originating from [U-13C]-glucose (A, B) and [U-13C]-glutamine (C).
FIGURE 4 from Mitochondria Transfer from Mesenchymal Stem Cells Confers Chemoresistance to Glioblastoma Stem Cells through Metabolic Rewiring
Authors: Nakhle Jean , Khattar Khattar , Özkan Tülin , Boughlita Adel , Abba Moussa Daouda , Darlix Amélie , Lorcy Frédérique , Rigau Valérie , Bauchet Luc , Gerbal-Chaloin Sabine , Daujat-Chavanieu Martine , Bellvert Floriant , Turchi Laurent , Virolle Thierry , Hugnot Jean-Philippe , Buisine Nicolas , Galloni Mireille , Dardalhon Valérie , Rodriguez Anne-Marie , Vignais Marie-Luce
Published: June 2023

MSC mitochondria modify GSC metabolite production in response to TMZ. GSC metabolites production was analyzed by mass spectrometry on whole-cell extracts. A, Heat map of metabolites produced by GSCs following acquisition of MSC mitochondria and/or TMZ treatment. B, MSEA in GSCs after mitochondria acquisition in comparison with control GSCs, without or with TMZ treatment. C, Metabolite concentrations for metabolic pathways identified in B. Two independent experiments were performed, each in triplicate. Each point corresponds to an individual culture and extraction. Values were normalized to cell numbers. Means ± SEM and t tests with Welch correction; *, P < 0.05; **, P < 0.01.
FIGURE 3 from Mitochondria Transfer from Mesenchymal Stem Cells Confers Chemoresistance to Glioblastoma Stem Cells through Metabolic Rewiring
Authors: Nakhle Jean , Khattar Khattar , Özkan Tülin , Boughlita Adel , Abba Moussa Daouda , Darlix Amélie , Lorcy Frédérique , Rigau Valérie , Bauchet Luc , Gerbal-Chaloin Sabine , Daujat-Chavanieu Martine , Bellvert Floriant , Turchi Laurent , Virolle Thierry , Hugnot Jean-Philippe , Buisine Nicolas , Galloni Mireille , Dardalhon Valérie , Rodriguez Anne-Marie , Vignais Marie-Luce
Published: June 2023

MSC mitochondria modify the metabolic response of GSCs to TMZ. MSC mitochondria were transferred by Mitoception to GSCs which were subsequently treated with TMZ (50 μmol/L) for 48 hours. A, Time line. Effects of TMZ in the presence/absence of MSC mitochondria on GSC OCRs (B and C) and ECARs (D and E). All values were normalized to GSC cell numbers. B, Representative plot of GSC OCR in basal conditions, treated with TMZ, MSC mitochondria or both, and after sequential addition of oligomycin, FCCP and rotenone/antimycin. Mean values and SEM (n = 4). C, Tukey boxplots showing basal respiration and maximal respiration (n = 25 from four independent experiments). One-way ANOVA; **, P < 0.01; ***, P < 0.001. D, Representative plot of GSC ECAR in basal conditions, treated with TMZ, MSC mitochondria or both, and after sequential addition of glucose, oligomycin, oxamate, and 2-deoxyglucose. Mean values and SEM (n = 5). E, Tukey boxplots showing GSC basal glycolysis (n = 25 from four independent experiments). One-way ANOVA; **, P < 0.01; ***, P < 0.001. F, OCR versus ECAR values of GSCs treated with TMZ, with MSC mitochondria or with both. Mean and SEM values. G–J, GSC mitochondrial mass and ROS production. GSCs labeled with MitoTracker and MitoSox were analyzed by FACS, following the acquisition of MSC mitochondria and 48 hours TMZ treatment. G, GSC total mitochondrial mass. Representative experiment and relative mitochondria mean fluorescence intensity values represented as mean ± SEM (n = 7). H, Expression of COX IV protein. Representative Western blots for COX IV and β−actin expression (MW markers in kDa). Quantifications (n = 3) represented as mean ± SEM. I, GSC ROS production as measured with Mitosox. Representative data and quantification from independent experiments (n = 9) with mean and SEM values. J, Ratios of GSC ROS production over mitochondrial mass (n = 7). G–J, One-way ANOVA; *, P < 0.05; **, P < 0.01; ***, P < 0.001.
FIGURE 2 from Mitochondria Transfer from Mesenchymal Stem Cells Confers Chemoresistance to Glioblastoma Stem Cells through Metabolic Rewiring
Authors: Nakhle Jean , Khattar Khattar , Özkan Tülin , Boughlita Adel , Abba Moussa Daouda , Darlix Amélie , Lorcy Frédérique , Rigau Valérie , Bauchet Luc , Gerbal-Chaloin Sabine , Daujat-Chavanieu Martine , Bellvert Floriant , Turchi Laurent , Virolle Thierry , Hugnot Jean-Philippe , Buisine Nicolas , Galloni Mireille , Dardalhon Valérie , Rodriguez Anne-Marie , Vignais Marie-Luce
Published: June 2023

MSC mitochondria increase GSC survival in response to TMZ. A and F, Time lines for the response of GSCs to TMZ, at day 5 (B–E) and at 48 hours (G–H). B, Survival of GSCs in response to TMZ (dose–response 6–400 μmol/L). Framed TMZ concentration of 50 μmol/L used in all subsequent experiments. C, Effect of TMZ on the transfer of mitochondria from MSCs to GSCs, as analyzed by flow cytometry. D and E, Effects of MSC mitochondria on GSC survival in response to TMZ. D, GSC caspase 3/7 activity (n = 16, three independent experiments). E, Cytotox assay (Incucyte). Representative images and quantification of Cytotox labeled GSCs (n = 15, 3 independent experiments). G and H, Effects of MSC mitochondria on GSC survival in response to TMZ (48 hours). H, GSC cell death. FACS analysis of Zombie violet-stained GSCs. Representative data and quantification from seven independent experiments with mean and SEM values. H, GSC cell number (n = 84 from seven independent experiments). Tukey boxplots with Kruskal–Wallis test; **, P < 0.01; ***, P < 0.001. C–E and G, Statistical analysis by one-way ANOVA; *, P < 0.05; ***, P < 0.001.
FIGURE 1 from Mitochondria Transfer from Mesenchymal Stem Cells Confers Chemoresistance to Glioblastoma Stem Cells through Metabolic Rewiring
Authors: Nakhle Jean , Khattar Khattar , Özkan Tülin , Boughlita Adel , Abba Moussa Daouda , Darlix Amélie , Lorcy Frédérique , Rigau Valérie , Bauchet Luc , Gerbal-Chaloin Sabine , Daujat-Chavanieu Martine , Bellvert Floriant , Turchi Laurent , Virolle Thierry , Hugnot Jean-Philippe , Buisine Nicolas , Galloni Mireille , Dardalhon Valérie , Rodriguez Anne-Marie , Vignais Marie-Luce
Published: June 2023

Exchange of mitochondria between MSCs with GSCs enhances GSC energy metabolism and proliferation. A and B, Mitochondria exchange during coculture of MSCs and GSCs, respectively prelabeled with red MitoTracker and green CellTracker. A, Imaging by confocal microscopy (24 hours). Scale bars: left, 20 μm; right, 5 μm. Arrows: MSC mitochondria. B, Quantification of mitochondria transfer to GSCs by flow cytometry analysis (48 hours coculture) Representative experiment and quantification with MSC from 3 donors. C–I, MSC mitochondria (three concentrations with 2-fold incremental increases) were transferred to GSCs by Mitoception and their effects on GSC functions were analyzed at 48 hours. C, Time line. D–H, Dose–response effects of MSC mitochondria on GSCs OCRs (D, E) and ECARs (F, G). All values were normalized to GSC cell numbers. D, Representative plot of GSC OCR in basal conditions and after sequential addition of oligomycin, Carbonyl cyanide-p-trifluoromethoxyphenylhydrazone (FCCP), and rotenone/antimycin. Mean values and SEM are indicated (n = 4). E, Tukey boxplots showing basal respiration, respiration linked to ATP production and maximal respiration. n = 18 from four independent experiments. One-way ANOVA; *, P < 0.05; ***, P < 0.001. F, Representative plot of GSC ECAR in basal conditions and after sequential addition of glucose, oligomycin, oxamate, and 2-deoxyglucose. Mean values and SEM are indicated (n = 6). G, Tukey boxplots showing basal glycolysis, glycolytic capacity, and lactate acidification. n = 13 from three independent experiments. One-way ANOVA; *, P < 0.05; **, P < 0.01; ***, P < 0.001. H, OCR versus ECAR of GSCs with MSC mitochondria. Mean and SEM. I, Tukey boxplots showing GSCs proliferation. One-way ANOVA; ***, P < 0.001. Data from B to I were obtained with MSCs from 3 donors.
The Diagnostic Value of miR-124a Expression in Peripheral Blood and Synovial Fluid of Patients with Rheumatoid Arthritis
Authors: Wu Tianhao , Zhang Yanlong , Peng Aqin , Wu Xirui
Published: June 2023

Introduction: Rheumatoid arthritis (RA), a chronic autoimmune disorder, is currently a severe health threat. Previous studies have documented the altered expression of various miRNAs in RA patients. This study determined the expression of miR-124a in RA patients and estimated its diagnostic value for RA. Methods: A total of 80 RA patients were enrolled as the study subjects, and 36 patients with osteoarthritis were included, with another 36 healthy people as the controls. miR-124a expression levels in peripheral blood plasma, peripheral blood mononuclear cells (PBMCs), and synovial fluid were measured using reverse transcription quantitative polymerase chain reaction, followed by Pearson correlation analysis. Additionally, the association between miR-124a and major clinical indicators was assessed, such as rheumatoid factor (RF), erythrocyte sedimentation rate (ESR), and disease activity score of 28 joints (DAS28). The diagnostic efficacy of miR-124a expression in plasma, PBMCs, and synovial fluid for RA was evaluated by the receiver operating characteristic curve, and the difference in the area under the curve (AUC) was analyzed. Results: miR-124a was downregulated in RA patients, and the expression levels of miR-124a in plasma, PBMCs, and synovial fluid showed a certain degree of positive correlation. miR-124a was inversely linked with RF, ESR, and DAS28. For the diagnosis of RA patients, the AUC of plasma miR-124a was 0.899 and the cut-off value was 0.800, with 68.75% sensitivity and 94.44% specificity; the AUC of miR-124a in PBMCs was 0.937 and the cut-off value was 0.805, with 82.50% sensitivity and 91.67% specificity; the AUC of miR-124a in plasma combined with PBMCs was 0.961, with a higher diagnostic value than independent plasma or PBMCs; the AUC of miR-124a in synovial fluid was 0.929 and the cut-off value was 0.835, with 80.00% sensitivity and 88.89% specificity. Conclusion: miR-124a expression is downregulated in the plasma, PBMCs, and synovial fluid of RA patients and has a high diagnostic value for RA.
Ambulatory Local Field Potential Recordings from the Thalamus in Epilepsy: A Feasibility Study
Authors: Satzer David , Wu Shasha , Henry Julia , Doll Emily , Issa Naoum P. , Warnke Peter C.
Published: June 2023

Introduction: Stimulation of the thalamus is gaining favor in the treatment of medically refractory multifocal and generalized epilepsy. Implanted brain stimulators capable of recording ambulatory local field potentials (LFPs) have recently been introduced, but there is little information to guide their use in thalamic stimulation for epilepsy. This study sought to assess the feasibility of chronically recording ambulatory interictal LFP from the thalamus in patients with epilepsy. Methods: In this pilot study, ambulatory LFP was recorded from patients who underwent sensing-enabled deep brain stimulation (DBS, 2 participants) or responsive neurostimulation (RNS, 3 participants) targeting the anterior nucleus of the thalamus (ANT, 2 electrodes), centromedian nucleus (CM, 7 electrodes), or medial pulvinar (PuM, 1 electrode) for multifocal or generalized epilepsy. Time-domain and frequency-domain LFP was investigated for epileptiform discharges, spectral peaks, circadian variation, and peri-ictal patterns. Results: Thalamic interictal discharges were visible on ambulatory recordings from both DBS and RNS. At-home interictal frequency-domain data could be extracted from both devices. Spectral peaks were noted at 10–15 Hz in CM, 6–11 Hz in ANT, and 19–24 Hz in PuM but varied in prominence and were not visible in all electrodes. In CM, 10–15 Hz power exhibited circadian variation and was attenuated by eye opening. Conclusion: Chronic ambulatory recording of thalamic LFP is feasible. Common spectral peaks can be observed but vary between electrodes and across neural states. DBS and RNS devices provide a wealth of complementary data that have the potential to better inform thalamic stimulation for epilepsy.
Gastrointestinal Eosinophilic Granulomatosis with Polyangiitis following a Clostridium difficile Infection
Authors: Mohideen Haseeb , Weldemichael Wegahta , Hussain Hafsa , Dahiya Dushyant Singh , Shin Andrea
Published: June 2023

Eosinophilic granulomatosis with polyangiitis (EGPA), historically named Churg-Strauss syndrome, is a rare vasculitis affecting small- and medium-sized blood vessels. The disease has a predilection for numerous organs including the lungs, sinuses, kidneys, heart, nerves, and gastrointestinal tract but is prominently associated with asthma, rhinosinusitis, and eosinophilia. Gastrointestinal involvement is common; however, a gastrointestinal manifestation as the cardinal symptom following an infection is atypical. Here, we present a case of a 61-year-old male who presented with persistent diarrhea following a toxigenic Clostridium difficile infection despite multiple antibiotic courses. Repeat testing confirmed eradication of the infection, and further evaluation with colon biopsy revealed small and medium-sized vasculitis with eosinophilic infiltration and granulomas. Treatment with prednisone and cyclophosphamide resulted in rapid improvement of his diarrhea. Gastrointestinal symptoms in EGPA are associated with worse prognosis, so prompt identification and treatment of the disease is crucial. EGPA is rarely documented in histopathological samples from the gastrointestinal tract as endoscopic biopsies are typically too superficial to sample the submucosal layer containing the affected vessels. Additionally, the link between EGPA and infections as a potential trigger has not been clearly established, but gastrointestinal EGPA manifesting after a colonic infection raises concerns that this may have been a triggering event. Ultimately, further study is needed to understand, diagnose, and treat gastrointestinal and postinfection EGPA.
Towards a New Definition of the Typical Day in the Alcohol Use Disorder Identification Test-Consumption
Authors: Esselink Annelien , Bovens Rob H.L.M. , Van de Mheen Dike H.M. , Gesthuizen Maurice J.W. , Mathijssen Jolanda J.P.
Published: June 2023

Introduction: The standard screening instrument for alcohol problems in the current primary care, the Alcohol Use Disorder Identification Test-Consumption (AUDIT-C), contains a question about alcohol consumption “on a typical day.” Since this is a term that leaves room for multiple interpretations and the differences between alcohol consumption on weekends and weekdays are not taken into account, this study examines whether the latter distinction improves the prediction validity of the screening instrument. Methods: A subgroup of 852 participants of the Dutch version of Dry January (“NoThanks”) 2022 got the annual “NoThanks” survey, including questions about their alcohol consumption on weekends and weekdays, and the original ten AUDIT questions. The full AUDIT was used as golden standard. Sensitivity, specificity, and receiver operating characteristic curves were calculated for the original and different versions of the AUDIT-C. Results: Of all participants, 67 percent were hazardous drinkers (AUDIT ≥8) and 27 percent were harmful drinkers (AUDIT ≥16). For the original AUDIT-C, the cut-off score with the most balanced combination of sensitivity and specificity for hazardous drinking in men was 7 and in women was 6. For harmful drinking, this was 8 and 7, respectively. Certain versions performed equally well as the original. For harmful drinkers, the highest area under the receiver operating characteristic curve (AUROC) was 0.814 and 0.866 for the original AUDIT-C, for men and women, respectively. Only for hazardous drinking men, the AUDIT-C with weekend day (AUROC = 0.887) performed slightly better than the original. Conclusion: Distinguishing weekend- and weekday alcohol consumption in the AUDIT-C does not lead to better predictions of problematic alcohol use. However, the distinction between weekends and weekdays provides more detailed information for healthcare professionals and can be used without having to compromise too much on validity.
Optimal Use of Serum Leucine-Rich Alpha-2 Glycoprotein as a Biomarker for Small Bowel Lesions of Crohn’s Disease
Authors: Asonuma Kunio , Kobayashi Taku , Kikkawa Nao , Nakano Masaru , Sagami Shintaro , Morikubo Hiromu , Miyatani Yusuke , Hojo Aya , Fukuda Tomohiro , Hibi Toshifumi
Published: June 2023

Introduction: A large proportion of small bowel lesions in Crohn’s disease (CD) may exist beyond the reach of ileocolonoscopy and there is no gold standard imaging modality to screen them, suggesting the need for optimal biomarkers. We aimed to compare the usefulness of C-reactive protein (CRP), faecal calprotectin (FC), and leucine-rich alpha-2 glycoprotein (LRG) in determining small bowel lesions of CD. Methods: This was a cross-sectional observational study. CRP, FC, and LRG were prospectively measured in patients with quiescent CD who underwent imaging examinations (capsule or balloon-assisted endoscopy, magnetic resonance enterography, or intestinal ultrasound) selected by the physician in clinical practice. Mucosal healing (MH) of the small bowel was defined as a lack of ulcers. Patients with a CD activity index >150 and active colonic lesions were excluded. Results: A total of 65 patients (27, MH; 38, small bowel inflammation) were analysed. The area under the curve (AUC) of CRP, FC, and LRG was 0.74 (95% confidence interval: 0.61–0.87), 0.69 (0.52–0.81), and 0.77 (0.59–0.85), respectively. The AUC of FC and LRG in a subgroup of 61 patients with CRP <3 mg/L (26, MH; 32, small bowel inflammation) was 0.68 (0.50–0.81) and 0.74 (0.54–0.84), respectively. The cut-off of 16 μg/mL of LRG showed the highest positive predictive value of 1.00 with specificity of 1.00, while negative predictive value was highest (0.71) with sensitivity of 0.89 at the cut-off of 9 μg/mL. Conclusion: LRG can accurately detect and/or exclude the small bowel lesions with two cut-off values.
Use of Talimogene Laherparepvec to Treat Cutaneous Squamous Cell Carcinoma in a Renal Transplant Patient
Authors: Miller Max , Kim Nancy H , Thosani Maya K , Moser Justin C
Published: June 2023

A 66-year-old female with a history of two renal transplants due to recurrent thrombotic thrombocytopenic purpura presented to clinic with multiple lesions identified to be non-metastatic cutaneous squamous cell carcinoma (CSCC). The patient previously underwent multiple Mohs procedures and radiation therapy treatment but continued to develop CSCC lesions with increasing frequency. After discussing multiple treatment options, it was elected to pursue treatment with Talimogene laherparepvec (T-VEC) given the systemic immune responses it can cause, with low theoretical risk of graft rejection. After starting intratumoral T-VEC injections, treated lesions began to decrease in size, and a reduction in the rate of new CSCC lesions was observed. Treatment was held due to unrelated renal complications during which time new CSCCs developed. Patient was restarted on T-VEC therapy with no recurrent renal issues. Upon reinitiating treatment, injected and non-injected lesions showed reduction in size, and the development of new lesions again ceased. One injected lesion was resected via Mohs micrographic surgery due to its size and discomfort. On sectioning, this demonstrated an exuberant lymphocytic perivascular infiltrate which was consistent with treatment response to T-VEC, with little active tumor. With high rates of non-melanoma skin cancer in renal transplant patients, their transplant status significantly limits treatment options, specifically with regards to anti-PD-1 therapy. This case suggests T-VEC can generate local and systemic immune responses in the setting of immunosuppression and that T-VEC may be a beneficial therapeutic option for transplant patients with CSCC.
Impact of Geographical Distance from Quaternary Treatment Center on Clinical Trial Participation, Intensive Induction Chemotherapy, and Outcomes in Patients with Newly Diagnosed Acute Myeloid Leukemia
Authors: Hershenfeld Samantha A. , Atenafu Eshetu G. , Chan Steven , Gupta Vikas , Maze Dawn , Schuh Andre , Sibai Hassan , Yee Karen , Schimmer Arron D.
Published: June 2023

Introduction: Care for patients with acute myeloid leukemia (AML) is centralized in the Ontario single-payer public healthcare system, with intensive induction chemotherapy and clinical trials only offered at specialized cancer centers with large catchment areas. Methods: We therefore conducted a retrospective single-center review of all AML patients assessed at a large specialized cancer center in Ontario, Canada. Results: Between 2012 and 2017, 1,310 patients were assessed by our center for upfront AML therapy. The median distance was 33.1 km, with 29% of patients living more than 50 km away from the center. There was no significant difference in probability of intensive induction chemotherapy or clinical trial by distance from center, both in univariate and multivariable analysis adjusting for age, sex, cytogenetics and molecular testing, and performance status. There was no significant difference in overall survival by distance from center on univariate and multivariable analysis. Conclusion: In conclusion, geographic distance from treatment center does not appear to impact choice of upfront therapy, participation in clinical trials, or clinical outcomes in this study of newly diagnosed patients with AML treated in a single-payer environment.
The Effects of Dexmedetomidine on Abdominal Aortic Occlusion-Induced Ovarian Injury via Oxidative Stress and Apoptosis
Authors: Mercantepe Filiz , Tumkaya Levent , Mercantepe Tolga , Akyildiz Kerimali , Ciftel Serpil , Yilmaz Adnan
Published: June 2023

Ischemia/reperfusion (I/R) induced ovarian damage is caused by various diseases such as ovarian torsion, ovarian transplantation, cardiovascular surgery, sepsis, or intra-abdominal surgery. I/R-related oxidative damage can impair ovarian functions, from oocyte maturation to fertilization. This study investigated the effects of dexmedetomidine (DEX), which has been shown to exhibit antiapoptotic, anti-inflammatory, and antioxidant effects, on ovarian I/R injury. We designed four study groups: group 1 (n = 6): control group; group 2 (n = 6): only DEX group; group 3 (n = 6): I/R group; group 4 (n = 6): I/R + DEX group. Then, ovarian samples were taken and examined histologically and immunohistochemically, and tissue malondialdehyde (MDA) and glutathione (GSH) levels were measured. In the I/R group MDA levels, caspase-3, NF-κB/p65, 8-OHdG positivity, and follicular degeneration, edema, and inflammation were increased compared to the control group (p = 0.000). In addition, GSH levels were significantly decreased in the I/R group compared to the control group (p = 0.000). On the other hand, in the I/R + DEX treatment group MDA levels, caspase-3, NF-κB/p65, 8-OHdG positivity, follicular degeneration, edema, and inflammation findings were decreased than in the I/R group (p = 0.000, p = 0.005, p = 0.005, p = 0.001, p = 0.005, respectively). However, GSH levels increased significantly in the I/R + DEX treatment group compared to the I/R group (p = 0.000). DEX protects against ovarian I/R injury through antioxidation and by suppressing inflammation and apoptosis.
Tempo, trabalho e transgressão no filme mexicano Almacenados de Jack Zagha Kababie
Authors: Garcia Wilton , Nascimento André , Maluly Luciano Victor Barros
Published: June 2023

Este texto desenvolve uma leitura crítico-reflexiva sobre o filme mexicano Almacenados, de Jack Zagha Kababie, na expectativa de pensar o sujeito contemporâneo e o tempo no trabalho. Este ensaio observa a articulação de estratégias discursivas dessa produção cultural, a qual compreende diferentes vertentes: literatura, teatro, cinema, música, entre outros. A base teórica aborda os estudos contemporâneos sob a produção de conhecimento, subjetividade e informação, ao relacionar atualização e inovação. Com isso, o percurso metodológico realiza-se em três níveis: observar, descrever e discutir a narrativa cinematográfica. Como resultado, interessa considerar, estrategicamente, na atualidade a partir dessa película algumas singularidades mexicanas que podem ser percebidas na América Latina.
A influência do contato com a natureza na reabilitação de pessoas em situação de dependência química residentes em Comunidades Terapêuticas
Authors: Bittencourt Rodolfo Nunes , Melo Junior João Carlos Ferreira de , Pedroso Mateus Vieira
Published: June 2023

Objetivo: identificar a influência da natureza sobre os sintomas de estresse, ansiedade e depressão em acolhidos em Comunidades Terapêuticas. Metodologia: estudo transversal, quali-quantitativo, exploratório e descritivo realizado com acolhidos em Comunidades Terapêuticas da região nordeste de Santa Catarina, em 2021. A saúde mental avaliada pela Escala de Depressão, Ansiedade e Estresse-21. Os participantes expressaram sua percepção utilizando três palavras que representaram os seus sentimentos com relação a esse benefício, criando-se uma Nuvens de Palavras. A análise de dados ocorreu por meio da estratificação das variáveis coletadas, sendo organizadas por frequências absolutas e relativas e testes estatísticos. Resultados: participaram 86 acolhidos do gênero masculino em 3 comunidades, idade média de 39,95 anos. Hipertensão Arterial Sistêmica foi a doença predominante; drogas de adição frequentes álcool e cocaína. Quanto aos sintomas, 53,4% (n=46) com sintomas de estresse, 41,7% (n=36) com sintomas de ansiedade e 34,8% (n=30) com sintomas depressivos. A Nuvem de Palavras apresentou os termos “bem-estar, calma e paz” como benefícios do contato com a natureza. Conclusão: os participantes apresentam índices de estresse, ansiedade e depressão elevados, porém consideram  o contato mais próximo com a natureza após o seu acolhimento na Comunidade Terapêutica como um fator positivo em sua reabilitação.
Coping styles of Guyanese nurses in the face of patients’ deaths: A cross-sectional study
Authors: Sampson Meshel Williams , Baldassarini Caíque Rossi , Oliveira Jaqueline Lemos de , Souza Jacqueline de
Published: June 2023

Objective: to investigate nurses’ strategies to cope with patients’ deaths and to explore potentialinfluences of cultural aspects on this phenomenon. Methodology: this is a cross-sectional study.The participants were 85 Registered Nurses from a Guyanese regional hospital. Data collectionwas carried out using a sociodemographic questionnaire and the Coping Strategies Inventoryby Lazarus and Folkman. Descriptive statistics and the Spearman’s correlation test, Student’st-test and Kruskal-Wallis test were performed to explore the data obtained. Results: most of theparticipants were women (85.9%) of African descent (56.5%). The mean age was 29.63 years old(SD=8.98), varying from 20 to 55 years old. Nurses have adopted the Planful problem-solving,Self-controlling and Positive reappraisal coping strategies to deal with patients’ deaths, and religionexerted an influence on the coping style they mentioned. Years as a Registered Nurse had positivecorrelations between the Planned problem-solving, Positive reappraisal, Seeking social support andDistancing coping styles related to patients’ deaths. Nurses professing the Hindu religion presentedhigher scores related to the Escape-avoidance coping style. Conclusion: even without specifictraining in coping with death, Guyanese nurses have adopted adequate coping strategies to dealwith this phenomenon. Cultural beliefs, such as religions of different philosophical and spiritualframeworks, may influence nurses’ coping strategies in the face of patients’ deaths.
Racial Differences in Presentation and Outcomes After Peripheral Arterial Interventions: Insights From the NCDR-PVI Registry
Authors: Julien Howard M. , Wang Yongfei , Curtis Jeptha P. , Johnston-Cox Hillary , Eberly Lauren A. , Wang Grace J. , Nathan Ashwin S. , Fanaroff Alexander C. , Khatana Sameed Ahmed M. , Groeneveld Peter W. , Secemsky Eric A. , Eneanya Nwamaka D. , Vora Amit N. , Kobayashi Taisei , Barbery Carlos , Chery Godefroy , Kohi Maureen , Kirksey Lee , Armstrong Ehrin J. , Jaff Michael R. , Giri Jay
Published: June 2023

Background:

We assess the rates of device use and outcomes by race among patients undergoing lower extremity peripheral arterial intervention using the American College of Cardiology National Cardiovascular Data Registry-Peripheral Vascular Intervention (PVI) registry.

Methods:

Patients who underwent PVI between April 2014 and March 2019 were included. Socioeconomic status was evaluated using the Distressed Community Index score for patients’ zip codes. Multivariable logistic regression was used to assess factors associated with utilization of drug-eluting technologies, intravascular imaging, and atherectomy. Among patients with Centers for Medicare and Medicaid Services data, we compared 1-year mortality, rates of amputation, and repeat revascularizations.

Results:

Of 63 150 study cases, 55 719 (88.2%) were performed in White patients and 7431 (11.8%) in Black patients. Black patients were younger (67.9 versus 70.0 years), had higher rates of hypertension (94.4% versus 89.5%), diabetes (63.0% versus 46.2%), less likely to be able to walk 200 m (29.1% versus 24.8%), and higher Distressed Community Index scores (65.1 versus 50.6). Black patients were provided drug-eluting technologies at a higher rate (adjusted odds ratio, 1.14 [95% CI, 1.06–1.23]) with no difference in atherectomy (adjusted odds ratio, 0.98 [95% CI, 0.91–1.05]) or intravascular imaging (adjusted odds ratio, 1.03 [95% CI, 0.88–1.22]) use. Black patients experienced a lower rate of acute kidney injury (adjusted odds ratio, 0.79 [95% CI, 0.72–0.88]). In Centers for Medicare and Medicaid Services-linked analyses of 7429 cases (11.8%), Black patients were significantly less likely to have surgical (adjusted hazard ratio, 0.40 [95% CI, 0.17–0.96]) or repeat PVI revascularization (adjusted hazard ratio, 0.42 [95% CI, 0.30–0.59]) at 1 year compared with White patients. There was no difference in mortality (adjusted hazard ratio [0.8–1.4]) or major amputation (adjusted hazard ratio, 2.5 [95% CI, 0.8–7.6]) between Black and White patients.

Conclusions:

Black patients presenting for PVI were younger, had higher prevalence of comorbidities and lower socioeconomic status. After adjustment, Black patients were less likely to have surgical or repeat PVI revascularization after the index PVI procedure.
Single-Cell Transcriptome Analysis of Peripheral Neutrophils From Patients With Idiopathic Pulmonary Arterial Hypertension
Authors: Zhang Rui , Zhang Ji , Zhang Yun-Long , Gong Su-Gang , Zhao Qin-Hua , Wang Xiao-Juan , Zhao Jia-Yu , Jiang Rong , Qiu Hong-Ling , Li Hui-Ting , He Jing , Liu Shao-Fei , Kuebler Wolfgang M. , Wang Lan
Published: June 2023

BACKGROUND:

Idiopathic pulmonary hypertension (IPAH) is a rare and devastating disease often accompanied by persistent inflammation and immune responses. We aim to provide a reference atlas of neutrophils to facilitate a better understanding of cellular phenotypes and discovery of candidate genes.

METHODS:

Peripheral neutrophils from naive patients with IPAH and matched controls were profiled. Whole-exon sequencing was performed to exclude known genetic mutations before establishing single-cell RNA sequencing. Marker genes were validated by flow cytometry and histology in a separate validation cohort.

RESULTS:

Seurat clustering analysis revealed that the landscape of neutrophils encompassed 5 clusters, including 1 progenitor, 1 transition, and 3 functional clusters. The intercorrelated genes in patients with IPAH were mainly enriched in antigen processing presentation and natural killer cell mediated cytotoxicity. We identified and validated differentially upregulated genes, including
MMP9
(matrix metallopeptidase 9),
ISG15
(ISG15 ubiquitin-like modifier), and
CXCL8
(C-X-C motif ligand 8). The positive proportions and fluorescence quantification of these genes were significantly increased in CD16
+
neutrophils in patients with IPAH. The higher proportion of positive MMP9 neutrophils increased mortality risk after adjustment for age and sex. Patients with higher proportions of positive MMP9 neutrophils had worse survival, while the fraction of ISG15- or CXCL8-positive expression neutrophils failed to predict outcome.

CONCLUSIONS:

Our study yields a comprehensive dataset of the landscape of neutrophils in patients with IPAH. The predictive values of a neutrophil cluster characterized by higher MMP9 expression indicate a functional role for neutrophil-specific matrix metalloproteinases in the pathogenesis of pulmonary arterial hypertension.
Prognostic Accuracy of N20 Somatosensory Potential in Patients With Acute Ischemic Stroke and Endovascular Thrombectomy
Authors: Martinez‐Piñeiro Alicia , Lucente Giuseppe , Hernandez‐Perez María , Cortés Jordi , Arbex Andrea , Pérez de la Ossa Natalia , Ramos‐Fransí Alba , Almendrote Miriam , Millán Mònica , Gomis Meritxell , Dorado Laura , Castaño Carlos , Remollo Sebastián , Cuadras Patricia , Garrido Alicia , Guanyabens Nicolau , Broto Joaquim , López‐Cancio Elena , Coll‐Canti Jaume , Dávalos Antoni ,   PROMISE (Somatosensory Evoked POtEntials MonItoring During Acute Ischemic StrokE) Study Group
Published: June 2023

Background

Somatosensory evoked potentials may add substantial prognostic value in patients with acute ischemic stroke and contribute to the selection of patients who may benefit from revascularization therapies beyond the accepted therapeutic time windows. We aimed to study the prognostic accuracy of the N20 somatosensory evoked potential component of the ischemic hemisphere in patients with anterior large‐vessel occlusion undergoing endovascular thrombectomy (EVT).

Methods

Presence and amplitude of the N20 response were recorded before and after EVT. Its adjusted predictive value for functional independence (modified Rankin scale score, ≤2) at day 7 was analyzed by binary logistic regression adjusting by age, mean arterial blood pressure, National Institute of Health Stroke Scale, Alberta Stroke Program Early CT Score, and serum glucose. N20 predictive power was compared with that of clinical and imaging models by using receiver operating characteristics curve analysis.

Results

A total of 223 consecutive patients were studied (mean age, 70 years; median National Institute of Health Stroke Scale score, 18). Somatosensory evoked potential recordings identified the presence of N20 in 110 (49.3%), absence in 58 (26%), and not assessable in 55 patients due to radiofrequency interferences in the angiography room. Before EVT, N20 predicted functional independence with a sensitivity of 93% (95% CI, 78%–98%) and negative predictive value of 93% (95% CI, 80%–98%). The adjusted odds ratio for functional independence was 9.9 (95% CI, 3.1–44.6). In receiver operating characteristics curve analysis, N20 amplitude showed a higher area under the curve than prehospital or in‐hospital variables, including advanced imaging. Sensitivity increased to 100% (95% CI, 0.85–1) when N20 was present after EVT.

Conclusion

Somatosensory evoked potential monitoring is a noninvasive and bedside technique that could help eligibility of patients with acute ischemic stroke for EVT and predict functional recovery.
Institutional Sanctions and Functionalism: Insights from the Most Recent Practice
Authors: Buscemi Martina
Published: June 2023

The article aims to discuss the most recent practice of institutional sanctions in light of contested fundamentals of the law of International Organizations (ios). The analysis delves, in particular, into sanctions adopted by ios having technical mandates in situations of wider political disputes among members, concerning international peace and security issues, amidst the United Nations (UN) Security Council’s failure to take coercive measures under Chapter vii of the UN Charter. Of special interest is the suspension of membership rights and privileges which some technical ios have deliberated following the conflict in Syria and the undergoing war in Ukraine (para. 2). These instances offer a test bed to challenge the fundamental theoretical paradigm of the law of ios, that is functionalism, and to extract some ambiguities underlying the essence of ios, revealing the continuous oscillation between policy and technocracy, which is endemic to the institutional life of every io and is well reflected in the different attitudes of the members and the Secretariats. The article highlights, in particular, the tension between politics and law that emerges from the adoption of sanctions by ios endowed with highly technical tasks (para. 3), the invocation of the ‘politicisation’ mantra by the targeted member to oppose the application of the privative measures (para. 4), and the ambivalent use of functionalist ‘language’ within the reacting io (para. 5). The article concludes with some final remarks on the ‘strategic’ use of ios (para. 6).
Singin’ in the Night
Authors: Muscianisi Domenico Giuseppe
Published: June 2023

Starting from present-day German Nachtigall ‘nightingale’, which shows an uncommon and unexpected i-linker, this article provides a morphological analysis of the word and its cognates. Through the frame of historical linguistics, and in particular the historical phonology and morphology of Proto-Germanic, it can be shown that Nachtigall and its Germanic cognates witness a relic word-formation that can be traced back to a delocatival compound in Proto-Indo-European. Comparative evidence allows for just such an etymology within poetic phraseology as well.