bluuebunny/crossref_metadata_2025 huggingface dataset:
Bioconjugation of Vegetable Oils with UV Absorbers: New Approach in Skin Photoprotection
Authors: Slabu Andrei Iulian , Miu Laura , Ghibu Emilian , Stavarache Cristina Elena , Stan Raluca , Teodorescu Florina
Published: November 2023

We reported the tunable synthesis of new vegetable oil–UV filter bioconjugates using sea buckthorn oil (SBO) and p-methoxycinnamic acid (p-MCA) as an alternative to the common UV filter, ethylhexyl-p-methoxycinnamate (octinoxate). The synthetic strategy is based on the sustainable ring-opening reaction of epoxidized SBO with p-MCA in heterogenous catalysis in eco-friendly solvents. The amount of UV-absorptive moieties grafted on the triglyceride backbone is controlled by different epoxidation degrees as determined by NMR spectroscopy. The performance of the new UV-absorber bioconjugates was assessed by in vitro sun protection factor (SPF) measurements after inclusion in SBO-ethylcellulose (EC) oleogels and comparison with the SPF value of the SBO-EC-octinoxate oleogel with equivalent p-MCA acid moieties (10% wt/wt). The concentration obtained for the SBO-EC oleogel formulated with the bioconjugate with the lowest degree of functionalization, namely 55%, represents 45% of the SPF determined for the SBO-EC-octinoxate oleogel, regardless of the concentration of measured solutions. The new concept of vegetable oil–UV-absorber bioconjugates has potential UV-B photoprotective properties when included in oleogel formulations and deserves further investigation of their properties and stability including association with UV-A absorbers, respectively.
Formation, Characterization, and Bonding of cis- and trans-[PtCl2{Te(CH2)6}2], cis-trans-[Pt3Cl6{Te(CH2)6}4], and cis-trans-[Pt4Cl8{Te(CH2)6}4]: Experimental and DFT Study
Authors: Rodewald Marko , Rautiainen J. Mikko , Görls Helmar , Oilunkaniemi Raija , Weigand Wolfgang , Laitinen Risto S.
Published: November 2023

[PtCl2{Te(CH2)6}2] (1) was synthesized from the cyclic telluroether Te(CH2)6 and cis-[PtCl2(NCPh)2] in dichloromethane at room temperature under the exclusion of light. The crystal structure determination showed that in the solid state, 1 crystallizes as yellow plate-like crystals of the cis-isomer 1cis and the orange-red interwoven needles of 1trans. The crystals could be separated under the microscope. NMR experiments showed that upon dissolution of the crystals of 1cis in CDCl3, it isomerizes and forms a dynamic equilibrium with the trans-isomer 1trans that becomes the predominant species. Small amounts of cis-trans-[Pt3Cl6{Te(CH2)6}4] (2) and cis-trans-[Pt4Cl8{Te(CH2)6}4] (3) were also formed and structurally characterized. Both compounds show rare bridging telluroether ligands and two different platinum coordination environments, one exhibiting a cis-Cl/cis-Te(CH2)6 arrangement and the other a trans-Cl/trans-Te(CH2)6 arrangement. Complex 2 has an open structure with two terminal and two bridging telluroether ligands, whereas complex 3 has a cyclic structure with four Te(CH2)6 bridging ligands. The bonding and formation of the complexes have been discussed through the use of DFT calculations combined with QTAIM analysis. The recrystallization of the mixture of the 1:1 reaction from d6-DMSO afforded [PtCl2{S(O)(CD3)2}{Te(CH2)6}] (4) that could also be characterized both structurally and spectroscopically.
Ionophore-Based Polymeric Sensors for Potentiometric Assay of the Anticancer Drug Gemcitabine in Pharmaceutical Formulation: A Comparative Study
Authors: Mostafa Gamal A. E. , El-Tohamy Maha F. , Ali Essam A. , Al-Salahi Rashad , Attwa Mohamed W. , AlRabiah Haitham
Published: November 2023

Gemcitabine is a chemotherapeutic agent used to treat various malignancies, including breast and bladder cancer. In the current study, three innovative selective gemcitabine hydrochloride sensors are developed using 4-tert-butylcalix-[8]-arene (sensor 1), β-cyclodextrin (sensor 2), and γ-cyclodextrin (sensor 3) as ionophores. The three sensors were prepared by incorporating the ionophores with o-nitrophenyl octyl ether as plasticizer and potassium tetrakis(4-chlorophenyl) borate as ionic additive into a polyvinyl chloride polymer matrix. These sensors are considered environmentally friendly systems in the analytical research. The linear responses of gemcitabine hydrochloride were in the concentration range of 6.0 × 10−6 to 1.0 × 10−2 mol L−1 and 9.0 × 10−6 to 1.0 × 10−2 mol L−1 and 8.0 × 10−6 to 1.0 × 10−2 mol L−1 for sensors 1, 2, and 3, respectively. Over the pH range of 6–9, fast-Nernst slopes of 52 ± 0.6, 56 ± 0.3, and 55 ± 0.8 mV/decade were found in the same order with correlation regressions of 0.998, 0.999, and 0.998, respectively. The lower limits of detection for the prepared sensors were 2.5 × 10−6, 2.2 × 10−6, and 2.7 × 10−6 mol L−1. The sensors showed high selectivity and sensitivity for gemcitabine. Validation of the sensors was carried out in accordance with the requirements established by the IUPAC, while being inexpensive and easy to use in drug formulation. A statistical analysis of the methods in comparison with the official method showed that there was no significant difference in accuracy or precision between them. It was shown that the new sensors could selectively and accurately find gemcitabine hydrochloride in bulk powder, pharmaceutical formulations, and quality control tests. The ionophore-based sensor shows several advantages over conventional PVC membrane sensor sensors regrading the lower limit of detection, and higher selectivity towards the target ion.
Effective Removal of Cd from Aqueous Solutions Using P-Loaded Ca-Mn-Impregnated Biochar
Authors: Qiu Cheng , Wang Chengwei , Liu Qinghai , Gao Minling , Song Zhengguo
Published: November 2023

Cadmium (Cd) pollution in wastewater has become an increasingly widespread concern worldwide. Studies on Cd (II) removal using phosphate-adsorbed sorbents are limited. This study aimed to elucidate the behaviors and mechanisms of Cd (II) sorption on phosphate-loaded Ca-Mn-impregnated biochar (Ps-CMBC). The Cd (II) sorption on Ps-CMBC reached equilibrium within 2 h and exhibited a higher sorption efficiency than biochar and CMBC. Additionally, the Langmuir isotherm could better describe the Cd (II) adsorption on the sorbents. P75-CMBC had a maximum Cd (II) sorption capability of 70.13 mg·g−1 when fitted by the Langmuir isotherm model, which was approximately 3.18 and 2.86 times greater than those of biochar and CMBC, respectively. Higher pH (5–7) had minimal effect on Cd (II) sorption capacity. The results of characterization analyses, such as SEM-EDS, FTIR, and XPS, suggested that there was a considerable difference in the sorption mechanisms of Cd (II) among the sorbents. The primary sorption mechanisms for biochar, CMBC, and Ps-CMBC included electrostatic attraction and surface complexation; additionally, for Ps-CMBC, Cd (II)-π interactions and coordination of Cd (II) with P=O were critical mechanisms for Cd (II) removal. The results of this study demonstrate that phosphate-loaded CMBC can be used as an effective treatment for heavy metal pollution in aqueous media.
Preparation and Photoelectric Properties of Pr-Doped p-Cu2O Thin Films Photocatalyst Based on Energy Band Structure Regulation
Authors: Wei Yuchen , Ji Qinggong , Wang Kai , Zhang Jian , Niu Jinfen , Yu Xiaojiao
Published: November 2023

A Pr-doped p-Cu2O thin film was prepared on indium tin oxide conductive glass by electrochemical deposition; the effect of Pr doping on the structure, morphology, and physicochemical properties of p-Cu2O was investigated. The results show that with the increase in Pr doping amount, the particle size of p-Cu2O increases, the absorption boundary redshifts, and the band-gap width decreases. Pr doping increases the flat band potential and carrier concentration of p-Cu2O; when the doping amount is 1.2 mM, the carrier concentration reaches 1.14 × 1024 cm−3. Compared with pure p-Cu2O, the charge transfer resistance of Pr-doped p-Cu2O decreases and the photocurrent and open circuit voltage increase, indicating that the carrier transfer rate is accelerated, and the separation efficiency of photogenerated electrons and holes is effectively improved. The result of a norfloxacin photocatalytic degradation experiment showed that the degradation rate of norfloxacin increased from 52.3% to 76.2% and Pr doping effectively improved the photocatalytic performance of p-Cu2O. The main reasons for enhancing the photocatalytic performance are that the band gap of Pr-doped p-Cu2O decreases, the Fermi level of Cu2O is closer to the valence band position, the hole concentration near the valence band, and the oxidation capacity increases, and more h+ oxidize norfloxacin molecules. In addition, the Pr in Pr-Cu2O acts as a conductor to guide electrons on the guide band to the crystal surface, which increases the contact between photogenerated electrons and dissolved oxygen, which is conducive to the formation of the active species ·O2− and can effectively reduce the recombination of photogenerated carriers. In the process of photocatalytic degradation of norfloxacin, the main active species are ·O2−, ·OH, and h+, which play auxiliary roles. TOC tests show that the norfloxacin molecules can be effectively degraded into small molecule organic matter, CO2, and H2O in the presence of Pr-doped p-Cu2O.
Detrimental Impacts of Pharmaceutical Excipient PEG400 on Gut Microbiota and Metabolome in Healthy Mice
Authors: Zhao Mei , Wang Pengjiao , Sun Xiaodong , Yang Dan , Zhang Shuo , Meng Xiaoxia , Zhang Min , Gao Xiuli
Published: November 2023

Polyethylene glycol 400 (PEG400) is a widely used pharmaceutical excipient in the field of medicine. It not only enhances the dispersion stability of the main drug but also facilitates the absorption of multiple drugs. Our previous study found that the long-term application of PEG400 as an adjuvant in traditional Chinese medicine preparations resulted in wasting and weight loss in animals, which aroused our concern. In this study, 16S rRNA high-throughput sequencing technology was used to analyze the diversity of gut microbiota, and LC-MS/MS Q-Exactive Orbtriap metabolomics technology was used to analyze the effect of PEG400 on the metabolome of healthy mice, combined with intestinal pathological analysis, aiming to investigate the effects of PEG400 on healthy mice. These results showed that PEG400 significantly altered the structure of gut microbiota, reduced the richness and diversity of intestinal flora, greatly increased the abundance of Akkermansia muciniphila (A. muciniphila), increased the proportion of Bacteroidetes to Firmicutes, and reduced the abundance of many beneficial bacteria. Moreover, PEG400 changed the characteristics of fecal metabolome in mice and induced disorders in lipid and energy metabolism, thus leading to diarrhea, weight loss, and intestinal inflammation in mice. Collectively, these findings provide new evidence for the potential effect of PEG400 ingestion on a healthy host.
Computational Design of Ni6@Pt1M31 Clusters for Multifunctional Electrocatalysts
Authors: Jia Jiaojiao , Tian Dongxu
Published: November 2023

High-efficiency and low-cost multifunctional electrocatalysts for hydrogen evolution reaction (HERs), oxygen evolution reaction (OERs) and oxygen reduction reaction (ORRs) are important for the practical applications of regenerative fuel cells. The activity trends of core–shell Ni6@M32 and Ni6@Pt1M31 (M = Pt, Pd, Cu, Ag, Au) were investigated using the density functional theory (DFT). Rate constant calculations indicated that Ni6@Pt1Ag31 was an efficient HER catalyst. The Volmer–Tafel process was the kinetically favorable reaction pathway for Ni6@Pt1M31. The Volmer–Heyrovsky reaction mechanism was preferred for Ni6@M32. The Pt active site reduced the energy barrier and changed the reaction mechanism. The ORR and OER overpotentials of Ni6@Pt1Ag31 were calculated to be 0.12 and 0.33 V, indicating that Ni6@Pt1Ag31 could be a promising multifunctional electrocatalyst. Ni6@Pt1M31 core–shell clusters present abundant active sites with a moderate adsorption strength for *H, *O, *OH and *OOH. The present study shows that embedding a single Pt atom onto a Ni@M core–shell cluster is a rational strategy for designing an effective multifunctional electrocatalyst.
Analysis of the Differences in Volatile Organic Compounds in Different Rice Varieties Based on GC-IMS Technology Combined with Multivariate Statistical Modelling
Authors: Chen Jin , Liu Ying , Yang Mi , Shi Xinmin , Mei Yuqin , Li Juan , Yang Chunqi , Pu Shihuang , Wen Jiancheng
Published: November 2023

In order to investigate the flavour characteristics of aromatic, glutinous, and nonaromatic rice, gas chromatography–ion mobility spectrometry (GC-IMS) was used to analyse the differences in volatile organic compounds (VOCs) amongst different rice varieties. The results showed that 103 signal peaks were detected in these rice varieties, and 91 volatile flavour substances were identified. Amongst them, 28 aldehydes (28.89~31.17%), 24 alcohols (34.85~40.52%), 14 ketones (12.26~14.74%), 12 esters (2.30~4.15%), 5 acids (7.80~10.85%), 3 furans (0.30~0.68%), 3 terpenes (0.34~0.64%), and 2 species of ethers (0.80~1.78%) were detected. SIMCA14.1 was used to perform principal component analysis (PCA) and orthogonal partial least squares discriminant analysis, and some potential character markers (VIP > 1) were further screened out of the 91 flavour substances identified based on the variable important projections, including ethanol, 1-hexanol, hexanal, heptanal, nonanal, (E)-2-heptenal, octanal, trans-2-octenal, pentanal, acetone, 6-methyl-5-hepten-2-one, ethyl acetate, propyl acetate, acetic acid, and dimethyl sulphide. Based on the established fingerprint information, combined with principal component analysis and orthogonal partial least squares discriminant analysis, different rice varieties were also effectively classified, and the results of this study provide data references for the improvement in aromatic rice varieties.
Protocol for Facile Synthesis of Fmoc-N-Me-AA-OH Using 2-CTC Resin as Temporary and Reusable Protecting Group
Authors: Román Tanya , Acosta Gerardo , Cárdenas Constanza , de la Torre Beatriz G. , Guzmán Fanny , Albericio Fernando
Published: November 2023

One approach to enhance the bioavailability and half-life of peptides in vivo is through N-methylation of one or more of the amino acids within the peptide sequence. However, commercially available Fmoc-N-Me-AA-OHs are limited and often expensive. In this study, a solid-phase synthesis method for Fmoc-N-Me-AA-OH was developed using a 2-chlorotrityl chloride (2-CTC) resin as a temporary protective group for the carboxylic acid strategy. Two strategies for the alkylation step were compared, employing either dimethyl sulfate or methyl iodide in the Biron−Kessler method. In this work we tested the protocol with two amino acids: Fmoc-Thr(tBu)-OH and Fmoc-βAla-OH. The first one is an alpha amino acid, very hindered and with the amine group directly influenced by the electronic effects of the carboxy group, whereas in Fmoc-βAla-OH, the presence of a methylene group weakens this influence due to the intervening carbon atoms. The desired amino acids, Fmoc-N-Me-Thr(tBu)-OH and Fmoc-N-Me-βAla-OH, were synthesized by both strategies with high yield and purity.
Novel Characterization Techniques for Multifunctional Plasmonic–Magnetic Nanoparticles in Biomedical Applications
Authors: Calvo Rodrigo , Rodriguez Mariblanca Isabel , Pini Valerio , Dias Monica , Cebrian Virginia , Thon Andreas , Saad Asis , Salvador-Matar Antonio , Ahumada Óscar , Manso Silván Miguel , Saunders Aaron E. , Wang Wentao , Stassinopoulos Adonis
Published: November 2023

In the rapidly emerging field of biomedical applications, multifunctional nanoparticles, especially those containing magnetic and plasmonic components, have gained significant attention due to their combined properties. These hybrid systems, often composed of iron oxide and gold, provide both magnetic and optical functionalities and offer promising avenues for applications in multimodal bioimaging, hyperthermal therapies, and magnetically driven selective delivery. This paper focuses on the implementation of advanced characterization methods, comparing statistical analyses of individual multifunctional particle properties with macroscopic properties as a way of fine-tuning synthetic methodologies for their fabrication methods. Special emphasis is placed on the size-dependent properties, biocompatibility, and challenges that can arise from this versatile nanometric system. In order to ensure the quality and applicability of these particles, various novel methods for characterizing the magnetic gold particles, including the analysis of their morphology, optical response, and magnetic response, are also discussed, with the overall goal of optimizing the fabrication of this complex system and thus enhancing its potential as a preferred diagnostic agent.
Influence of Grain Size on Dielectric Behavior in Lead-Free 0.5 Ba(Zr0.2Ti0.8)O3–0.5 (Ba0.7Ca0.3)TiO3 Ceramics
Authors: Ene Vladimir Lucian , Lupu Valentin Razvan , Condor Claudiu Vasile , Patru Roxana Elena , Hrib Luminita Mirela , Amarande Luminita , Nicoara Adrian Ionut , Pintilie Lucian , Ianculescu Adelina-Carmen
Published: November 2023

Fine-tuning of grain sizes can significantly influence the interaction between different dielectric phenomena, allowing the development of materials with tailored dielectric resistivity. By virtue of various synthesis mechanisms, a pathway to manipulate grain sizes and, consequently, tune the material’s dielectric response is revealed. Understanding these intricate relationships between granulation and dielectric properties can pave the way for designing and optimizing materials for specific applications where tailored dielectric responses are sought. The experimental part involved the fabrication of dense BCT-BZT ceramics with different grain sizes by varying the synthesis (conventional solid-state reaction route and sol-gel) and consolidation methods. Both consolidation methods produced well-crystallized specimens, with Ba0.85Ca0.15O3Ti0.9Zr0.1 (BCTZ) perovskite as the major phase. Conventional sintering resulted in microstructured and submicron-structured BCT-BZT ceramics, with average grain sizes of 2.35 μm for the solid-state sample and 0.91 μm for the sol-gel synthesized ceramic. However, spark plasma sintering produced a nanocrystalline specimen with an average grain size of 67.5 nm. As the grain size decreases, there is a noticeable decrease in the maximum permittivity, a significant reduction in dielectric losses, and a shifting of the Curie temperature towards lower values.
Selective Laser-Assisted Direct Synthesis of MoS2 for Graphene/MoS2 Schottky Junction
Authors: Jeon Min Ji , Hyeong Seok-Ki , Jang Hee Yoon , Mun Jihun , Kim Tae-Wook , Bae Sukang , Lee Seoung-Ki
Published: November 2023

Implementing a heterostructure by vertically stacking two-dimensional semiconductors is necessary for responding to various requirements in the future of semiconductor technology. However, the chemical-vapor deposition method, which is an existing two-dimensional (2D) material-processing method, inevitably causes heat damage to surrounding materials essential for functionality because of its high synthesis temperature. Therefore, the heterojunction of a 2D material that directly synthesized MoS2 on graphene using a laser-based photothermal reaction at room temperature was studied. The key to the photothermal-reaction mechanism is the difference in the photothermal absorption coefficients of the materials. The device in which graphene and MoS2 were vertically stacked using a laser-based photothermal reaction demonstrated its potential application as a photodetector that responds to light and its stability against cycling. The laser-based photothermal-reaction method for 2D materials will be further applied to various fields, such as transparent display electrodes, photodetectors, and solar cells, in the future.
Donor-Acceptor Copolymers with 9-(2-Ethylhexyl)carbazole or Dibenzothiophene-5,5-dioxide Donor Units and 5,6-Difluorobenzo[c][1,2,5]thiadiazole Acceptor Units for Photonics
Authors: Cimrová Věra , Babičová Petra , Guesmi Mariem , Výprachtický Drahomír
Published: November 2023

Semiconducting polymers, particularly of the third generation, including donor-acceptor (D-A) copolymers, are extensively studied due to their huge potential for photonic and electronic applications. Here, we report on two new D-A copolymers, CP1 and CP2, composed of different electron-donor (D) units: 9-(2-ethylhexyl)carbazole or dibenzothiophene-5,5-dioxide, respectively, and of 4,7-bis(4′-(2-octyldodecyl)thiophen-2′-yl)-5,6-difluorobenzo[c][1,2,5]thiadiazole building block with central 5,6-difluorobenzo[c][1,2,5]thiadiazole electron-acceptor (A) units, which were synthesized by Suzuki coupling in the high-boiling solvent xylene and characterized. The copolymers exhibited very good thermal and oxidation stability. A copolymer CP1 with different molecular weights was prepared in order to facilitate a comparison of CP1 with CP2 of comparable molecular weight and to reveal the relationship between molecular weight and properties. The photophysical, electrochemical, and electroluminescence properties were examined. Intense red photoluminescence (PL) with higher PL efficiencies for CP1 than for CP2 was observed in both solutions and films. Red shifts in the PL thin film spectra compared with the PL solution spectra indicated aggregate formation in the solid state. X-ray diffraction measurements revealed differences in the arrangement of molecules in thin films depending on the molecular weight of the copolymers. Light-emitting devices with efficient red emission and low onset voltages were prepared and characterized.
An Overview of the Immune Modulatory Properties of Long Non-Coding RNAs and Their Potential Use as Therapeutic Targets in Cancer
Authors: Martinez-Castillo Moises , M. Elsayed Abdelrahman , López-Berestein Gabriel , Amero Paola , Rodríguez-Aguayo Cristian
Published: November 2023

Long non-coding RNAs (lncRNAs) play pivotal roles in regulating immune responses, immune cell differentiation, activation, and inflammatory processes. In cancer, they are gaining prominence as potential therapeutic targets due to their ability to regulate immune checkpoint molecules and immune-related factors, suggesting avenues for bolstering anti-tumor immune responses. Here, we explore the mechanistic insights into lncRNA-mediated immune modulation, highlighting their impact on immunity. Additionally, we discuss their potential to enhance cancer immunotherapy, augmenting the effectiveness of immune checkpoint inhibitors and adoptive T cell therapies. LncRNAs as therapeutic targets hold the promise of revolutionizing cancer treatments, inspiring further research in this field with substantial clinical implications.
Continuous Monitoring of Interstitial Fluid Glucose Responses to Endurance Exercise with Different Levels of Carbohydrate Intake
Authors: Hiromatsu Chiyori , Kasahara Naoto , Lin Chao-An , Wang Feifei , Goto Kazushige
Published: November 2023

We compared the 24 h changes in interstitial fluid glucose concentration (IGC) following a simulated soccer match between subjects consuming a high-carbohydrate (HCHO; 8 g/kg BW/day) diet and those consuming a moderate-carbohydrate (MCHO; 4 g/kg BW/day) diet. Eight active healthy males participated in two different trials. The subjects were provided with the prescribed diets from days 1 to 3. On day 3, the subjects performed 90 min (2 bouts × 45 min) of exercise simulating a soccer match. The IGC of the upper arm was continuously monitored from days 1 to 4. No significant difference in the IGC was observed between trials during exercise. The total area under the curve (t-AUC) value during exercise did not significantly differ between the HCHO (9719 ± 305 mg/dL·90 min) and MCHO (9991 ± 140 mg/dL·90 min). Serum total ketone body and beta-hydroxybutyrate concentrations were significantly higher in the MCHO than in the HCHO after a second bout of exercise. No significant differences in the IGC were observed between trials at any time point during the night after exercise (0:00–7:00). In addition, t-AUC value during the night did not significantly differ between the HCHO (32,378 ± 873 mg/dL·420 min) and MCHO (31,749 ± 633 mg/dL·420 min). In conclusion, two days of consuming different carbohydrate intake levels did not significantly affect the IGC during a 90 min simulated soccer match. Moreover, the IGC during the night following the exercise did not significantly differ between the two trials despite the different carbohydrate intake levels (8 vs. 4 g/kg BW/day).
Esculeogenin A, a Glycan from Tomato, Alleviates Nonalcoholic Fatty Liver Disease in Rats through Hypolipidemic, Antioxidant, and Anti-Inflammatory Effects
Authors: Al Jadani Jwharah M. , Albadr Nawal A. , Alshammari Ghedeir M. , Almasri Soheir A. , Alfayez Farah Fayez , Yahya Mohammed Abdo
Published: November 2023

This study examined the preventative effects of esculeogenin A (ESGA), a newly discovered glycan from tomato, on liver damage and hepatic steatosis in high-fat-diet (HFD)-fed male rats. The animals were divided into six groups (each of eight rats): a control group fed a normal diet, control + ESGA (200 mg/kg), HFD, and HFD + ESAG in 3 doses (50, 100, and 200 mg/kg). Feeding and treatments were conducted for 12 weeks. Treatment with ESGA did not affect gains in the body or fat weight nor increases in fasting glucose, insulin, and HOMA-IR or serum levels of free fatty acids (FFAs), tumor-necrosis factor-α, and interleukin-6 (IL-6). On the contrary, it significantly reduced the serum levels of gamma-glutamyl transpeptidase (GGT), aspartate aminotransferase (AST), alanine aminotransferase (ALT), total triglycerides (TGs), cholesterol (CHOL), and low-density lipoprotein cholesterol (LDL-c) in the HFD-fed rats. In addition, it improved the liver structure, attenuating the increase in fat vacuoles; reduced levels of TGs and CHOL, and the mRNA levels of SREBP1 and acetyl CoA carboxylase (ACC); and upregulated the mRNA levels of proliferator-activated receptor α (PPARα) and carnitine palmitoyltransferase I (CPT I) in HFD-fed rats. These effects were concomitant with increases in the mRNA, cytoplasmic, and nuclear levels of nuclear factor erythroid 2-related factor 2 (Nrf2), glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), and heme oxygenase-1 (HO); a reduction in the nuclear activity of nuclear factor-kappa beta (NF-κB); and inhibition of the activity of nuclear factor kappa B kinase subunit beta (IKKβ). All of these effects were dose-dependent effects in which a normal liver structure and normal levels of all measured parameters were seen in HFD + ESGA (200 mg/kg)-treated rats. In conclusion, ESGA prevents NAFLD in HFD-fed rats by attenuating hyperlipidemia, hepatic steatosis, oxidative stress, and inflammation by acting locally on Nrf2, NF-κB, SREBP1, and PPARα transcription factors.
Metabolic Syndrome and Rheumatoid Arthritis Activity: An Analysis of Clinical, Laboratory, and Ultrasound Parameters
Authors: Grzechnik Krzysztof , Targońska-Stępniak Bożena
Published: November 2023

(1) Background: Rheumatoid arthritis (RA) is a chronic autoimmune disease associated with an increased incidence of metabolic syndrome (MetS). The aim of this study was to determine if there is an association between MetS and parameters of RA activity, as well as between metabolic parameters and indices of RA activity. (2) Methods: This study involved 65 patients with RA. MetS was diagnosed according to the 2009 IDF/AHA/NHLBI criteria. The comparative analysis was conducted between RA patients with MetS (RA (MetS (+)) and without MetS (RA (MetS (−)). The activity of RA was assessed using clinical, laboratory, and ultrasound (US) parameters. (3) Results: Compared with RA MetS (−) patients, RA MetS (+) patients were characterized by higher disease activity, according to Disease Activity Score (DAS28), Simplified Disease Activity Index (SDAI), and Clinical Disease Activity Index (CDAI). RA MetS (+) patients had significantly higher tender and swollen joint counts, and values of erythrocyte sedimentation rate, C-reactive protein, and US parameters (grey-scale (GSUS), power Doppler (PDUS)). Significant correlations were found between metabolic parameters (waist circumference, cholesterol and glucose concentrations) and indices of RA activity. (4) Conclusion: The results of this study show that, in patients with RA, the presence of MetS is associated with higher disease activity, based on several clinical, laboratory, and US parameters.
The Role of Epigenetic Control of Mitochondrial (Dys)Function in MASLD Onset and Progression
Authors: Caputo Valerio , Tarantino Giovanni , Santini Silvano Junior , Fracassi Giovanna , Balsano Clara
Published: November 2023

Metabolic dysfunction-associated steatotic fatty liver disease (MASLD), a novel definition for NAFLD, represents one of the most common causes of liver disease, and its incidence is increasing worldwide. It is characterized by a complex etiopathogenesis in which mitochondrial dysfunction exerts a pivotal role together with alteration of lipid metabolism, inflammation, and oxidative stress. Nutrients and bioactive compounds can influence such mechanisms so that changes in diet and lifestyle are regarded as important treatment strategies. Notably, natural compounds can exert their influence through changes of the epigenetic landscape, overall resulting in rewiring of molecular networks involved in cell and tissue homeostasis. Considering such information, the present review aims at providing evidence of epigenetic modifications occurring at mitochondria in response to natural and bioactive compounds in the context of liver (dys)function. For this purpose, recent studies reporting effects of compounds on mitochondria in the context of NAFLD/MASLD, as well as research showing alteration of DNA methylation and non-coding RNAs-related circuits occurring at liver mitochondria, will be illustrated. Overall, the present review will highlight the importance of understanding the bioactive compounds-dependent epigenetic modulation of mitochondria for improving the knowledge of MASLD and identifying biomarkers to be employed for effective preventative strategies or treatment protocols.
The Synergistic Effect of Quince Fruit and Probiotics (Lactobacillus and Bifidobacterium) on Reducing Oxidative Stress and Inflammation at the Intestinal Level and Improving Athletic Performance during Endurance Exercise
Authors: Herrera-Rocha Karen Marlenne , Manjarrez-Juanes María Magdalena , Larrosa Mar , Barrios-Payán Jorge Alberto , Rocha-Guzmán Nuria Elizabeth , Macías-Salas Alejo , Gallegos-Infante José Alberto , Álvarez Saul Alberto , González-Laredo Rubén Francisco , Moreno-Jiménez Martha Rocío
Published: November 2023

Endurance exercise promotes damage at the intestinal level and generates a variety of symptoms related to oxidative stress processes, inflammatory processes, microbiota dysbiosis, and intestinal barrier damage. This study evaluated the effects of quince (Cydonia oblonga Mill.) and probiotics of the genera Lactobacillus and Bifidobacterium on intestinal protection and exercise endurance in an animal swimming model. Phytochemical characterization of the quince fruit demonstrated a total dietary fiber concentration of 0.820 ± 0.70 g/100 g and a fiber-bound phenolic content of 30,218 ± 104 µg/g in the freeze-dried fruit. UPLC-PDA-ESI-QqQ analyses identified a high content of polyphenol, mainly flavanols, hydroxycinnamic acids, hydroxybenzoic acids, flavonols, and, to a lesser extent, dihydrochalcones. The animal model of swimming was performed using C57BL/6 mice. The histological results determined that the consumption of the synbiotic generated intestinal protection and increased antioxidant (catalase and glutathione peroxidase enzymes) and anti-inflammatory (TNF-α and IL-6 and increasing IL-10) activities. An immunohistochemical analysis indicated mitochondrial biogenesis (Tom2) at the muscular level related to the increased swimming performance. These effects correlated mainly with the polyphenol content of the fruit and the effect of the probiotics. Therefore, this combination of quince and probiotics could be an alternative for the generation of a synbiotic product that improves exercise endurance and reduces the effects generated by the practice of high performance sports.
Efficacy of Violet-Blue (405 nm) LED Lamps for Disinfection of High-Environmental-Contact Surfaces in Healthcare Facilities: Leading to the Inactivation of Microorganisms and Reduction of MRSA Contamination
Authors: Amodeo Davide , Manzi Pietro , De Palma Isa , Puccio Alessandro , Nante Nicola , Barcaccia Mariella , Marini Daniele , Pietrella Donatella
Published: November 2023

Effective disinfection procedures in healthcare facilities are essential to prevent transmission. Chemical disinfectants, hydrogen peroxide vapour (HPV) systems and ultraviolet (UV) light are commonly used methods. An emerging method, violet-blue light at 405 nm, has shown promise for surface disinfection. Its antimicrobial properties are based on producing reactive oxygen species (ROS) that lead to the inactivation of pathogens. Studies have shown significant efficacy in reducing bacterial levels on surfaces and in the air, reducing nosocomial infections. The aim of this study was to evaluate the antimicrobial effectiveness of violet-blue (405 nm) LED lamps on high-contact surfaces in a hospital infection-control laboratory. High-contact surfaces were sampled before and after 7 days of exposure to violet-blue light. In addition, the effect of violet-blue light on MRSA-contaminated surfaces was investigated. Exposure to violet-blue light significantly reduced the number of bacteria, yeasts and moulds on the sampled surfaces. The incubator handle showed a low microbial load and no growth after irradiation. The worktable and sink showed an inconsistent reduction due to shaded areas. In the second experiment, violet-blue light significantly reduced the microbial load of MRSA on surfaces, with a greater reduction on steel surfaces than on plastic surfaces. Violet-blue light at 405 nm has proven to be an effective tool for pathogen inactivation in healthcare settings Violet-blue light shows promise as an additional and integrated tool to reduce microbial contamination in hospital environments but must be used in combination with standard cleaning practices and infection control protocols. Further research is needed to optimise the violet-blue, 405 nm disinfection method.
Testing Effects of Seed Treatments against Clubroot Disease in Various Oilseed Rape Hybrids
Authors: Klueken A. , Mahfoud Yamen , Rößler Sabine , Ludwig-Müller Jutta
Published: November 2023

Clubroot disease, caused by the protist pathogen Plasmodiophora brassicae, is an emerging threat to cruciferous crops, including oilseed rape (Brassica napus L.). Most of the current commercial cultivars are highly susceptible, and efficient management tools are lacking practical implementation. Over three years and three experimental periods, we studied the effects of isotianil in comparison with Bacillus amyloliquefaciens QST713-HiCFU against clubroot disease under greenhouse experiments. Our results show control effects, which were strongly dependent on seasons, host plant genotype, and clubroot isolates: isotianil and B. amyloliquefaciens QST713-HiCFU reduced disease severity consistently at variable, but field-relevant spore concentrations of clubroot isolates; with seed treatments showing superior effects compared to drench applications. The co-application of isotianil with B. amyloliquefaciens QST713-HiCFU could, in some cases, increase the efficacy. Interestingly, all studied hybrids reacted to treatments, albeit to a somewhat different extent. When tested against a field isolate, the results obtained with the single spore isolate were partially confirmed but with greater variability. Overall, the generally positive effects of isotianil and B. amyloliquefaciens QST713-HiCFU on the reduction of clubroot were repeatedly observed. The inoculation of clubroot disease with different spore counts indicates a dose–response effect for tested products. This study highlights the importance of performing experiments holistically over multiple, consecutive seasons, with various isolates, application types, and different genetic resources of host plants.
Brain Delivery of Cisplatin Using Microbubbles in Combination with Ultrasound as an Effective Therapy for Glioblastoma
Authors: Hagiwara Fumiko , Omata Daiki , Munakata Lisa , Kageyama Saori , Maruyama Kazuo , Kudo Nobuki , Suzuki Ryo
Published: November 2023

Glioblastoma is a highly invasive and fatal disease. Temozolomide, a blood–brain barrier (BBB)-penetrant therapeutic agent currently used for glioblastoma, does not exhibit sufficient therapeutic effect. Cisplatin (CDDP), a versatile anticancer drug, is not considered a therapeutic option for glioblastoma due to its low BBB permeability. We previously investigated the utility of microbubbles (MBs) in combination with ultrasound (US) in promoting BBB permeability and reported the efficacy of drug delivery to the brain using a minimally invasive approach. This study aimed to evaluate the feasibility of CDDP delivery to the brain using the combination of MBs and US for the treatment of glioblastoma. We used mice that were implanted with glioma-261 GFP-Luc cells expressing luciferase as the glioblastoma model. In this model, after tumor inoculation, the BBB opening was induced using MBs and US, and CDDP was simultaneously administered. We found that the CDDP concentrations were higher at the glioblastoma site where the US was applied, although CDDP normally cannot pass through the BBB. Furthermore, the survival was longer in mice treated with CDDP delivered via MBs and US than in those treated with CDDP alone or those that were left untreated. These results suggest that the combination of MBs and US is an effective antitumor drug delivery system based on BBB opening in glioblastoma therapy.
Docetaxel-Loaded Methoxy poly(ethylene glycol)-poly (L-lactic Acid) Nanoparticles for Breast Cancer: Synthesis, Characterization, Method Validation, and Cytotoxicity
Authors: Miraj Shumaila , Saeed Hamid , Iqtedar Mehwish , Albekairi Norah A. , Ahmed Nadeem , Danish Muhammad Zeeshan , Islam Muhammad , Rasool Muhammad Fawad , Deen Kashif Mairaj , Rathore Hassaan Anwer
Published: November 2023

This study aimed to synthesize and characterize DTX-mPEG-PLA-NPs along with the development and validation of a simple, accurate, and reproducible method for the determination and quantification of DTX in mPEG-PLA-NPs. The prepared NPs were characterized using AFM, DLS, zetasizer, and drug release kinetic profiling. The RP-HPLC assay was developed for DTX detection. The cytotoxicity and anti-clonogenic effects were estimated using MTT and clonogenic assays, respectively, using both MCF-7 and MDA-MB-231 cell lines in a 2D and 3D culture system. The developed method showed a linear response, high precision, accuracy, RSD values of ≤2%, and a tailing factor ≤2, per ICH guidelines. The DTX-mPEG-PLA-NPs exhibited an average particle size of 264.3 nm with an encapsulation efficiency of 62.22%. The in vitro drug kinetic profile, as per the Krosmeyers–Peppas model, demonstrated Fickian diffusion, with initial biphasic release and a multistep sustained release over 190 h. The MTT assay revealed improved in vitro cytotoxicity against MCF-7 and MDA-MB-231 in the 2D cultures and MCF-7 3D mammosphere cultures. Significant inhibitions of the clonogenic potential of MDA-MB-231 were observed for all concentrations of DTX-mPEG-PLA-NPs. Our results highlight the feasibility of detecting DTX via the robust RP-HPLC method and using DTX-mPEG-PLA-NPs as a perceptible and biocompatible delivery vehicle with greater cytotoxic and anti-clonogenic potential, supporting improved outcomes in BC.
Enhancing Therapeutic Drug Monitoring in Inflammatory Bowel Disease: A Comparative Analysis of Rapid Point-of-Care Infliximab, Adalimumab and Anti-Drug Antibodies’ Determination against ELISA
Authors: Toja-Camba Francisco José , García-Quintanilla Laura , Rodríguez-Martinez Lorena , Tomine Julia , Cajade-Pascual Francisco , Feitosa Carolina , Zarra-Ferro Irene , Barreiro-De-Acosta Manuel , González-López Jaime , Mondelo-García Cristina , Fernández-Ferreiro Anxo
Published: November 2023

The introduction of point-of-care (POC) assays into clinical practice in patients with inflammatory disease enables on-demand therapeutic decision making. The aim of this study was to compare the POC test Quantum blue (Bühlmann Laboratories) for infliximab (IFX), adalimumab (ADL), and its anti-drug antibodies with the traditional ELISA assay (Promonitor). A total of 200 serum samples were analyzed. Samples were classified into the following three different groups; sub-therapeutic range (IFX < 3 μg/mL and ADL < 5 μg/mL); therapeutic range (IFX: 3–7 μg/mL and ADL: 5–12 μg/mL) and supra-therapeutic range (IFX levels > 7 μg/mL and ADL levels > 12 μg/mL). Significant higher values were measured using the POC test (p < 0.001) for IFX results but no differences in ADL trough levels were observed (p = 0.3101). Spearman’s correlation indicated a good correlation between the two assays (rs = 0.88 for ADL and rs = 0.93 for IFX), and McNemar’s test revealed significant differences (p = 0.016) when classifying IFX samples between therapeutic and supra-therapeutic ranges but no significant differences were found among the other ranges for either IFX or ADL. These results show that we should be cautious when using these rapid measurement methods, and new targets should probably be defined for IFX when using this new analytical method.
Colorectal Cancer: Disease Process, Current Treatment Options, and Future Perspectives
Authors: Adebayo Amusa S. , Agbaje Kafilat , Adesina Simeon K. , Olajubutu Oluwabukunmi
Published: November 2023

Colorectal cancer (CRC) is one of the deadliest malignancies in the US, ranking fourth after lung, prostate, and breast cancers, respectively, in general populations. It continues to be a menace, and the incidence has been projected to more than double by 2035, especially in underdeveloped countries. This review seeks to provide some insights into the disease progression, currently available treatment options and their challenges, and future perspectives. Searches were conducted in the PubMed search engine in the university’s online library. The keywords were “Colorectal Cancer” AND “disease process” OR “disease mechanisms” OR “Current Treatment” OR “Prospects”. Selection criteria were original articles published primarily during the period of 2013 through 2023. Abstracts, books and documents, and reviews/systematic reviews were filtered out. Of over 490 thousand articles returned, only about 800 met preliminary selection criteria, 200 were reviewed in detail, but 191 met final selection criteria. Fifty-one other articles were used due to cross-referencing. Although recently considered a disease of lifestyle, CRC incidence appears to be rising in countries with low, low–medium, and medium social demographic indices. CRC can affect all parts of the colon and rectum but is more fatal with poor disease outcomes when it is right-sided. The disease progression usually takes between 7–10 years and can be asymptomatic, making early detection and diagnosis difficult. The CRC tumor microenvironment is made up of different types of cells interacting with each other to promote the growth and proliferation of the tumor cells. Significant advancement has been made in the treatment of colorectal cancer. Notable approaches include surgery, chemotherapy, radiation therapy, and cryotherapy. Chemotherapy, including 5-fluorouracil, irinotecan, oxaliplatin, and leucovorin, plays a significant role in the management of CRC that has been diagnosed at advanced stages. Two classes of monoclonal antibody therapies have been approved by the FDA for the treatment of colorectal cancer: the vascular endothelial growth factor (VEGF) inhibitor, e.g., bevacizumab (Avastin®), and the epidermal growth factor receptor (EGFR) inhibitor, e.g., cetuximab (Erbitux®) and panitumumab (Verbitix®). However, many significant problems are still being experienced with these treatments, mainly off-target effects, toxic side effects, and the associated therapeutic failures of small molecular drugs and the rapid loss of efficacy of mAb therapies. Other novel delivery strategies continue to be investigated, including ligand-based targeting of CRC cells.
The Role of Collision Ionization of K-Shell Ions in Nonequilibrium Plasmas Produced by the Action of Super Strong, Ultrashort PW-Class Laser Pulses on Micron-Scale Argon Clusters with Intensity up to 5 × 1021 W/cm2
Authors: Skobelev Igor Yu. , Ryazantsev Sergey N. , Kulikov Roman K. , Sedov Maksim V. , Filippov Evgeny D. , Pikuz Sergey A. , Asai Takafumi , Kanasaki Masato , Yamauchi Tomoya , Jinno Satoshi , Ota Masato , Egashira Syunsuke , Sakai Kentaro , Minami Takumi , Abe Yuki , Tokiyasu Atsushi , Kohri Hideki , Kuramitsu Yasuhiro , Sakawa Youichi , Miyasaka Yasuhiro , Kondo Kotaro , Kon Akira , Sagisaka Akito , Ogura Koichi , Pirozhkov Alexander S. , Kando Masaki , Kiriyama Hiromitsu , Pikuz Tatiana A. , Fukuda Yuji
Published: November 2023

The generation of highly charged ions in laser plasmas is usually associated with collisional ionization processes that occur in electron–ion collisions. An alternative ionization channel caused by tunnel ionization in an optical field is also capable of effectively producing highly charged ions with ionization potentials of several kiloelectronvolts when the laser intensity q > 1020 W/cm2. It is challenging to clearly distinguish the impacts of the optical field and collisional ionizations on the evolution of the charge state of a nonequilibrium plasma produced by the interaction of high-intensity, ultrashort PW-class laser pulses with dense matter. In the present work, it is shown that the answer to this question can be obtained in some cases by observing the X-ray spectral lines caused by the transition of an electron into the K-shell of highly charged ions. The time-dependent calculations of plasma kinetics show that this is possible, for example, if sufficiently small clusters targets with low-density background gas are irradiated. In the case of Ar plasma, the limit of the cluster radius was estimated to be R0 = 0.1 μm. The calculation results for argon ions were compared with the results of the experiment at the J-KAREN-P laser facility at QST-KPSI.
Optically Reconfigurable THz Metamaterial with Switchable Wideband Absorption and Transmission
Authors: Wang Liansheng , Li Qingge , Fu Quanhong , Ding Xueyong , Wang Yuan , Zhu Weiren
Published: November 2023

We present an optically reconfigurable multifunctional metamaterial with switchable wideband absorption and transmission across the THz frequency range. The reconfigurability is achieved by introducing optically active silicon into the resonator of the metamaterial unit which could be effectively modulated through external electrical pumping. When the silicon conductivity reaches 50,000 S/m, the metamaterial shows significant absorption of electromagnetic waves ranging from 3.1 to 10 THz. By reducing the applied external pump power and decreasing the silicon conductivity to 150 S/m, the proposed metamaterial exhibits transmission of the incident waves at 5.12 THz with a transmission coefficient of 61%. The proposed optically reconfigurable metamaterial has the advantages of simple structure, wideband absorption, and switchable between absorption and transmission modes, showing potential applications in stealth, communication, or radar systems.