bluuebunny/crossref_metadata_2025 huggingface dataset:
On use of equations of motion for two-rotor airship
Authors: Adamski Wojciech , Herman Przemysław
Published: December 2011

In this article, equations of motion for a two-rotor airship are derived. Moreover, the problem of engine applied forces and torques is analysed, and a trajectory generator is described and implemented into a proportional–derivative control scheme. The theoretical considerations are validated by a set of simulation tests. The simulation model takes into account the structure of the drives, namely the two engines placed symmetrically on both sides of the object. The conducted simulations validate the robustness of the controller in the case of unexpected disturbance occurrence.
Investigation of a new flux scheme for the numerical simulation of the supersonic intake flow
Authors: Soltani M R , Younsi J S , Farahani M
Published: December 2011

A numerical code for supersonic intake design with a proper simulation of the normal and/or oblique shocks, boundary layer development, interaction of the shock and the boundary layer, as well as prediction of the flow separation is of great help to the designers. In this research, a numerical code is developed to solve the inner and outer flow fields of the intake and validated with various experimental tests. The intake is an axisymmetric external compression one. Roe scheme and new schemes, AUSM+-up (for all speed) and Advection Upstream Splitting Method with Pressure-Based Weight function (AUSMPW), are used to compute the convective fluxes. The original version of the AUSMPW scheme has serious problems concerning the stability and accuracy. In this investigation, several modifications are implemented to the original version of the AUSMPW scheme for the first time which resulted in a better stability and accuracy. Turbulent viscosity coefficient is computed using the Baldwin–Lomax algebraic model. The numerical results are compared well with the experimental data. It is further found that the Roe scheme has the best accuracy; however, the AUSM+-up (for all speed) scheme is numerically very efficient.
Intake ground vortex characteristics
Authors: Mishra N , MacManus D , Murphy J
Published: December 2011

The development of ground vortices when an intake operates in close proximity to the ground has been studied computationally for several configurations including front and rear quarter approaching flows as well as tailwind arrangements. The investigations have been conducted at model scale using a generic intake geometry. Reynolds Averaged Navier–Stokes calculations have been used and an initial validation of the computational model has been carried out against experimental data. The computational method has subsequently been applied to configurations that are difficult to test experimentally by including tailwind and rear quarter flows. The results, along with those from a previous compatible study of headwind and pure cross-wind configurations, have been used to assess the ground vortex behaviour under a broad range of velocity ratios and approaching wind angles. The characteristics provide insights on the influence of the size and strength of ground vortices on the overall quality of the flow ingested by the intake.
Chris Abani’s Graceland and Uzodinma Iweala’s Beasts of No Nation: Nonstandard English, intertextuality and Ken Saro-Wiwa’s Sozaboy
Authors: Lambert Iain
Published: December 2011

This article explores the use of nonstandard English forms and intertextuality in two recent works by Nigerian writers in English living abroad. To date, Chris Abani’s Graceland and Uzodinma Iweala’s Beasts of No Nation have attracted little critical commentary, far less any academic survey of their language, yet each book is in its own way representative of conflicting treatments of nonstandard varieties of Nigerian English by writers in the diaspora. Beasts of No Nation owes a considerable debt to the linguistic and stylistic experiments Ken Saro-Wiwa made in his novel Sozaboy and Iweala has drawn heavily on this work in his use of a first person narrator and his assignment of a limited, if forcefully expressive, language to his hero. According to Saro-Wiwa, Sozaboy is written in a mixture of Nigerian Pidgin (NP), Standard English (SE) and other forms. Graceland, however, makes selective use of nonstandard forms for reasons closer to those of earlier writers and makes this clear through its author’s insertion of intertextual elements. After providing an overview of the background to and characteristic features of NP and Nigerian English this article surveys their use in Nigerian literature and concludes by examining the language of Graceland and Beasts of No Nation through a linguistic comparison of shared episodes and a consideration of thematic similarities in order to place these two novels in a continuum of Nigerian writing in English through their use of language.
‘Whatever form you spoke of you were right’: Multivalence and ambiguous address in Cormac McCarthy’s The Road
Authors: Warde Anthony
Published: December 2011

This article explores the workings of second-person pronoun forms in Cormac McCarthy’s 2006 post-apocalyptic novel The Road. More particularly, the analysis focuses on examples of ‘doubly deictic you’ (Herman, 2002), and demonstrates how the novel exploits the uncertain deictic, referential and address functions of this particular pronoun form to develop what I term a ‘post-apocalyptic poetics’, through which it attempts to explore – and enact – the spatial and temporal dislocations that ensue from the fictional apocalypse. The article also demonstrates how the novel’s indeterminate use of narrative you creates profound hermeneutical (and often ontological) uncertainty for readers, who must often suspend any attempt to fix the positions from and to which the story is addressed. McCarthy’s opaque use of the terms you and your throughout the novel creates profound polyphony and multivalence by preventing readers from clearly distinguishing the discourse and perspectives of protagonists from those of the narration, and by thus impelling readers to develop several interpretations of key passages, all of which must be sustained simultaneously. Finally, the analysis explores how the (potential) apostrophic effects associated with doubly deictic you serve to immerse readers in the horrors of the post-apocalyptic world, thus increasing the novel’s ecocritical import.
Videoconferencing as a Mode of Communication
Authors: Denstadli Jon Martin , Julsrud Tom Erik , Hjorthol Randi Johanne
Published: December 2011

Based on a quantitative survey of Norwegian business travelers, this study compares their use of face-to-face (FTF) meetings and videoconferences (VCs). The study finds that access and use of VCs are determined mainly by industry and the geographical structure of the enterprise. It also finds that VCs and FTF meetings differ along several dimensions, suggesting that these two modes of communication fulfill slightly different needs. Based on the survey results, the authors propose a framework to understand the emerging role of VCs. This framework would address both relational and task-based dimensions.
A review of long-term memory in natural and synthetic systems
Authors: Wood Rachel , Baxter Paul , Belpaeme Tony
Published: December 2011

Memory may be broadly regarded as information gained from past experience that is available in the service of ongoing and future adaptive behavior. The biological implementation of memory shares little with memory in synthetic cognitive systems where it is typically regarded as a passive storage structure. Neurophysiological evidence indicates that memory is neither passive nor centralized. A review of the relevant literature in the biological and computer sciences is conducted and a novel methodology is applied that incorporates neuroethological approaches with general biological inspiration in the design of synthetic cognitive systems: a case study regarding episodic memory provides an illustration of the utility of this methodology. As a consequence of applying this approach to the reinterpretation of the implementation of memory in synthetic systems, four fundamental functional principles are derived that are in accordance with neuroscientific theory, and which may be applied to the design of more adaptive and robust synthetic cognitive systems: priming, cross-modal associations, cross-modal coordination without semantic information transfer, and global system behavior resulting from activation dynamics within the memory system.
Papillary Thyroid Carcinoma– Like Tumor of the Kidney
Authors: Khoja Hatim A. , Almutawa Abdulmonem , Binmahfooz Ali , Aslam Muhammad , Ghazi Abdullah A. , Almaiman Sara
Published: December 2011

Thyroid carcinoma–like tumor of the kidney is an extremely rare variant of renal cell carcinoma. Most previously reported cases were incidental finding; and none of them showed papillary thyroid carcinoma (PTC) nuclear features. This study reports the first case of PTC (follicular variant)–like tumor of the kidney in which a female patient presented with hematuria, weight loss, and flank pain. Imaging studies revealed a left renal mass with enlarged hilar lymph nodes. Histologically, the renal tumor had a striking resemblance to follicular variant of PTC. However, no radiological abnormalities were found in the thyroid, mediastinum, or pelvis. Tumor cells were negative for thyroid markers (thyroglobulin and TTF1). According to the authors, this is the first case of PTC (follicular variant)–like tumor of the kidney.
Factors in Client–Clinician Interaction That Influence Hearing Aid Adoption
Authors: Poost-Foroosh Laya , Jennings Mary Beth , Shaw Lynn , Meston Christine N. , Cheesman Margaret F.
Published: December 2011

The influence of client–clinician interactions has not been emphasized in hearing health care, despite the extensive evidence of the impact of the provider–patient interaction on health outcomes. The purpose of this study was to identify factors in the client–clinician interaction that may influence hearing aid adoption. Thirteen adults who had received a hearing aid recommendation within the previous 3 months and 10 audiologists participated in a study to generate, sort, and rate the importance of factors in client–clinician interaction that may influence the hearing aid purchase decision. A concept mapping approach was used to define meaningful clusters of factors. Quantitative analysis and qualitative interpretation of the statements resulted in eight concepts. The concepts in order of their importance are (a) Ensuring client comfort, (b) Understanding and meeting client needs, (c) Client-centered traits and actions, (d) Acknowledging client as an individual, (e) Imposing undue pressure and discomfort, (f) Conveying device information by clinician, (g) Supporting choices and shared decision making, and (h) Factors in client readiness. Two overarching themes of client-centered interaction and client empowerment were identified. Results highlight the influence of the client–clinician interaction in hearing aid adoption and suggest the possibility of improving hearing aid adoption by empowering clients through a client-centered interaction.
From Harare to Rio de Janeiro: Kukiya-Favela organization of the excluded
Authors: Imas J. Miguel , Weston Alia
Published: December 2011

This article, based on ethnographic research conducted with people in Brazil and Zimbabwe, reports organization/management experiences and narratives of poor and marginalized people of the south. South embodies the organizational struggle, survival skills and resilience of marginal and urban outcasts that inhabit inner cities, townships and slums. The article employs the notion of kukiya-favela organization, i.e. the organization of the excluded, to engage with them in order to: give voice to those who dwell at the margins of organization studies; make their narratives part of a subject that retains an elitist position; and re-address the Eurocentric management/organization discourse that imposes a legitimate justification for exploiting, excluding and labelling them as organization-less and urban outcasts of society. The article concludes that despite their marginality and exclusion they are able to construct local diverse meaningful (organizational) identities that can represent them with dignity in their struggle for justice and basic human rights. Finally, it reflects on the contribution this has for us, in organization studies, by opening new spaces for the study of organization[al] (lives) not from positions of ‘above’ or ‘against’ but ‘with’ ( Gergen, 2003 : 454).
Intonational Patterns of Nonverbal Vocalizations in People With Dementia
Authors: Samuelsson Christina , Hydén Lars C.
Published: December 2011

Nonverbal vocalizations in dementia are important clinically since they generally have been regarded as disruptive behavior that is disturbing. The aim of the present study is to describe the interactional pattern, including the prosodic package, of nonverbal vocalizations in a participant in a late stage of dementia. The acoustic analysis shows that the vocalizations do not differ significantly from the verbal utterances regarding mean fundamental frequency or pitch range. The mean fundamental frequency, F0, of the utterances from Anna was significantly higher than the mean F0 from the other elderly participants. The analysis demonstrates that there is a singing-like type of vocalizations that does not resemble the previously described patterns of nonverbal vocalizations. This pattern of the nonverbal vocalization does not resemble the intonation of Anna’s verbal utterances. The other participants perceive Anna’s vocalizations as potentially meaningful turns. Nonverbal vocalizations in clinical settings should be taken as communicative contributions.
Cerebral Intolerance During Flow Arrested Carotid Angioplasty
Authors: Louis Myron St. , Park Brian D. , Dahn Michael , Bozeman Patricia
Published: December 2011

Introduction: The use of flow arrest as a means of providing cerebral protection during carotid angioplasty offers the advantages of improved efficiency of debris removal and the ability to provide protection under unfavorable (tortuous) anatomic circumstances. However, in contrast to the filtration methods of cerebral protection, this modality requires complete interruption of antegrade carotid artery flow during balloon angioplasty and stent deployment. Methods: We report our experience with 9 patients undergoing carotid angioplasty with the Mo.Ma device, which utilizes common and external carotid artery balloon occlusion during the angioplasty procedure. We assessed the clinical outcomes and intraprocedural hemodynamic data. Results: The average duration of carotid occlusion was 8.3 minutes. Of the 9 patients, 2 patients (22%) experienced cerebral intolerance. No stroke occurred in this patient cohort. There appeared to be a poor relationship between procedure intolerance and the presence of significant contralateral stenosis or low carotid back pressure. Furthermore, the incidence of postangioplasty hypotension was not clearly related to cerebral intolerance. Conclusion: Carotid angioplasty with stenting can be safely conducted with flow arrest as an alternative to filter-type cerebral protection devices. However, because cerebral intolerance is not an infrequent occurrence with this approach, clinicians must be cognizant of management strategies for transient cerebral intolerance.
Review of Indications and Practices of Vena Caval Filters at a Large University Hospital
Authors: Meisner Robert J. , Labropoulos Nicos , Gasparis Antonios P. , Lampl Jenica , Xu Min , Tassiopoulos Apostolos K.
Published: December 2011

Background: Vena caval filter (VCF) use has been increasing in recent years. Prophylactic VCF placement has been applied liberally in high-risk patients. Methods: Consecutive patients with VCF placement over a 2-year period at a university hospital were reviewed. Results: A total of 244 patients underwent VCF placement in 2 years. Of all, 54% of the patients had the VCF placed for an absolute indication, 14% for a relative indication, and 32% for prophylaxis. Only 14 (9%) of the retrievable filters were removed. Eight patients had a complication of VCF placement; there were no complications of filter retrieval. Vena caval filter placement for prophylaxis alone was 57% from the division of trauma and surgical critical care, 18.3% from interventional radiology department, and 5.2% from the division of vascular surgery. Conclusions: This study indicates that many VCFs are placed for prophylaxis. A low percentage of VCFs was retrieved. This may be the practice at many other large university-based hospitals, necessitating strategies for reducing their placement.
Routine Venography Following Transaxillary First Rib Resection and Scalenectomy (FRRS) for Chronic Subclavian Vein Thrombosis Ensures Excellent Outcomes and Vein Patency
Authors: Chang Kevin Z. , Likes Kendall , Demos Jasmine , Black James H. , Freischlag Julie A.
Published: December 2011

To assess the role of postoperative venography in patients treated with first rib resection and scalenectomy (FRRS) for effort thrombosis, a retrospective review was done to evaluate long-term venous patency in 84 patients treated at the Johns Hopkins Medical Institutions. Patients undergo venography 2 weeks postoperatively. If there is >50% stenosis, the subclavian vein is dilated and the patient receives anticoagulation. If the vein is occluded, patients are maintained on anticoagulation. Of the 85 patients, 21 patients had patent veins, 47 patients had stenotic veins, and 16 patients had chronically occluded veins. In follow-up, symptomatic restenosis was seen in 3 patients and those veins were redilated. Two other patients had late occlusions at 23 and 63 months and received anticoagulation and redilatation, respectively. Using venography to guide postoperative management, 79 of 84 patients had patent veins many years postoperatively. Long-term patency, as seen by duplex scan, was achieved in nearly all patients using this protocol.
Results of Radiofrequency Ablation of the Small Saphenous Vein in the Supine Position
Authors: Monahan Thomas S. , Belek Kyle , Sarkar Rajabrata
Published: December 2011

Objectives: To report the results of a novel approach using supine positioning for radiofrequency ablation (RFA) of the small saphenous vein (SSV) with combined ablation of the great saphenous vein (GSV). Methods: Over a 24-month period, we identified patients with symptomatic SSV incompetence. Access to the SSV was accomplished by ultrasound-guided venipuncture with the patient in the supine position. Results: Small saphenous vein ablation was performed on 27 limbs in 26 patients. Median follow-up was 94 days (interquartile range [IQR] 26, 171). Mean clinical–etiologic–anatomic–pathophysiologic (CEAP) score was 3.5 ± 1.3. Small saphenous vein ablation was performed in conjunction with GSV ablation in 17 patients and with phlebectomy in 14 patients. Postoperative ultrasound was performed after 26 of 27 procedures. The SSV was sealed in all 26 cases. Two patients (8%) had a deep venous thrombosis (DVT). Conclusions: The SSV can be effectively sealed by RFA from the supine position and combined SSV/GSV ablation can be carried out in a single setting.
A visual analytics framework for strategic airlift decision making
Authors: Soban Danielle S , Salmon John , Fahringer Phil
Published: December 2011

The foundational concept of Network Enabled Capability relies on effective, timely information sharing. This information is used in analysis, trade and scenario studies, and ultimately decision making. In this paper, the concept of visual analytics is explored as an enabler to facilitate rapid, defensible and superior decision making. By coupling analytical reasoning with the exceptional human capability to rapidly internalize and understand visual data, visual analytics allows individual and collaborative decision making to occur in the face of vast and disparate data, time pressures and uncertainty. An example visual analytics framework is presented in the form of a decision-making environment centered on the Lockheed C-5A and C-5M aircraft. This environment allows rapid trade studies to be conducted on design, logistics and capability within the aircraft’s operational roles. Through this example, the use of a visual analytics decision-making environment within a military environment is demonstrated.
Evaluation of Bioabsorbable Seamguard for Staple Line Reinforcement in Stapled Rectal Anastomoses
Authors: Fajardo Alyssa D. , Amador-Ortiz Catalina , Chun Jonathan , Stewart David , Fleshman James W.
Published: December 2011

Introduction. The concept of staple line reinforcement is a growing area of interest. This study evaluated the feasibility and effect of using bioabsorbable Seamguard (BSG) to bolster end-to-end stapled rectal anastomoses in a porcine model. Methods. Eleven female 45-kg Yucatan domestic pigs were used. Each animal served as its own control by creating a BSG and nonreinforced anastomosis using a 29-mm end-to-end anastomotic stapling device. Reinforced anastomoses were randomized to proximal and distal positions along the rectum. Each staple line reinforcement agent consisted of adding BSG to the stapling device according to the manufacturer’s instructions. Barium enemas were then performed and the 2 anastomotic sites harvested. Each anastomosis underwent burst testing. The internal diameter of each anastomosis was measured and underwent pathologic review. Results. Bolstered anastomoses offered no strength advantage as burst pressures were no different as compared with unbolstered anastomoses. There was also no difference in anastomotic internal or external diameters. Only 1 stapled anastomosis burst during testing and none in the bolstered group. On histological analysis, there was a significant increase in inflammatory infiltrate in the bolstered group as compared with the stapled group ( P = .041), with a higher incidence of lymphocytes ( P = .047) and giant cells ( P = .037). There was no difference in mucosal loss at the anastomotic site, neovascularization, fibroblast presence, extent of fibrosis, muscle layer disruption, percentage of anastomosis replaced by collagen, and elastin deposition. Conclusions. The routine use of BSG bolsters in stapled rectal anastomoses is safe and results in equivalent anastomotic strength as traditional stapled anastomoses.
Independent Animation, Rotoshop and Communities of Practice: As Seen Through A Scanner Darkly
Authors: Ward Paul
Published: December 2011

The article examines a particular instance of animation practice through a reading of how Bob Sabiston’s Rotoshop software was used in the 2006 film A Scanner Darkly. By discussing the notions of ‘communities of practice’ and ‘legitimate peripheral participation’, and contextualizing the film in relation to different modes of working, the author excavates the ways in which a range of people came to work on the project. Moreover, he outlines some of the production history of the film to argue that certain assumptions and expectations about accepted working practice point to wider perceptions of ‘independent’ and ‘studio’ animation. Questions of division of labour and standardization, and how they relate to creativity, autonomy and animation production will be addressed; Rotoshop’s position in the history of animation forms an interesting case study for interrogating these issues.
A small molecule screening strategy with validation on human leukemia stem cells uncovers the therapeutic efficacy of kinetin riboside
Authors: McDermott Sean P. , Eppert Kolja , Notta Faiyaz , Isaac Methvin , Datti Alessandro , Al-awar Rima , Wrana Jeff , Minden Mark D. , Dick John E.
Published: December 2011

Gene regulatory networks that govern hematopoietic stem cells (HSCs) and leukemia-initiating cells (L-ICs) are deeply entangled. Thus, the discovery of compounds that target L-ICs while sparing HSC is an attractive but difficult endeavor. Presently, most screening approaches fail to counter-screen compounds against normal hematopoietic stem/progenitor cells (HSPCs). Here, we present a multistep in vitro and in vivo approach to identify compounds that can target L-ICs in acute myeloid leukemia (AML). A high-throughput screen of 4000 compounds on novel leukemia cell lines derived from human experimental leukemogenesis models yielded 80 hits, of which 10 were less toxic to HSPC. We characterized a single compound, kinetin riboside (KR), on AML L-ICs and HSPCs. KR demonstrated comparable efficacy to standard therapies against blast cells in 63 primary leukemias. In vitro, KR targeted the L-IC–enriched CD34+CD38− AML fraction, while sparing HSPC-enriched fractions, although these effects were mitigated on HSC assayed in vivo. KR eliminated L-ICs in 2 of 4 primary AML samples when assayed in vivo and highlights the importance of in vivo L-IC and HSC assays to measure function. Overall, we provide a novel approach to screen large drug libraries for the discovery of anti–L-IC compounds for human leukemias.
B-cell depletion and remissions of malignancy along with cytokine-associated toxicity in a clinical trial of anti-CD19 chimeric-antigen-receptor–transduced T cells
Authors: Kochenderfer James N. , Dudley Mark E. , Feldman Steven A. , Wilson Wyndham H. , Spaner David E. , Maric Irina , Stetler-Stevenson Maryalice , Phan Giao Q. , Hughes Marybeth S. , Sherry Richard M. , Yang James C. , Kammula Udai S. , Devillier Laura , Carpenter Robert , Nathan Debbie-Ann N. , Morgan Richard A. , Laurencot Carolyn , Rosenberg Steven A.
Published: December 2011

We conducted a clinical trial to assess adoptive transfer of T cells genetically modified to express an anti-CD19 chimeric Ag receptor (CAR). Our clinical protocol consisted of chemotherapy followed by an infusion of anti–CD19-CAR–transduced T cells and a course of IL-2. Six of the 8 patients treated on our protocol obtained remissions of their advanced, progressive B-cell malignancies. Four of the 8 patients treated on the protocol had long-term depletion of normal polyclonal CD19+ B-lineage cells. Cells containing the anti-CD19 CAR gene were detected in the blood of all patients. Four of the 8 treated patients had prominent elevations in serum levels of the inflammatory cytokines IFNγ and TNF. The severity of acute toxicities experienced by the patients correlated with serum IFNγ and TNF levels. The infused anti–CD19-CAR–transduced T cells were a possible source of these inflammatory cytokines because we demonstrated peripheral blood T cells that produced TNF and IFNγ ex vivo in a CD19-specific manner after anti–CD19-CAR–transduced T-cell infusions. Anti–CD19-CAR–transduced T cells have great promise to improve the treatment of B-cell malignancies because of a potent ability to eradicate CD19+ cells in vivo; however, reversible cytokine-associated toxicities occurred after CAR–transduced T-cell infusions. This trial was registered with ClinicalTrials.gov as NCT00924326.
Cloning and characterization of a pectin lyase gene from Colletotrichum lindemuthianumand comparative phylogenetic/structural analyses with genes from phytopathogenic and saprophytic/opportunistic microorganisms
Authors: Lara-Márquez Alicia , Zavala-Páramo María G , López-Romero Everardo , Calderón-Cortés Nancy , López-Gómez Rodolfo , Conejo-Saucedo Ulises , Cano-Camacho Horacio
Published: December 2011

Background

Microorganisms produce cell-wall-degrading enzymes as part of their strategies for plant invasion/nutrition. Among these, pectin lyases (PNLs) catalyze the depolymerization of esterified pectin by a β-elimination mechanism. PNLs are grouped together with pectate lyases (PL) in Family 1 of the polysaccharide lyases, as they share a conserved structure in a parallel β-helix. The best-characterized fungal pectin lyases are obtained from saprophytic/opportunistic fungi in the generaAspergillusandPenicilliumand from some pathogens such asColletotrichum gloeosporioides.

The organism used in the present study,Colletotrichum lindemuthianum, is a phytopathogenic fungus that can be subdivided into different physiological races with different capacities to infect its host,Phaseolus vulgaris. These include the non-pathogenic and pathogenic strains known as races 0 and 1472, respectively.

Results

Here we report the isolation and sequence analysis of theClpnl2gene, which encodes the pectin lyase 2 ofC. lindemuthianum, and its expression in pathogenic and non-pathogenic races ofC. lindemuthianumgrown on different carbon sources. In addition, we performed a phylogenetic analysis of the deduced amino acid sequence of Clpnl2 based on reported sequences of PNLs from other sources and compared the three-dimensional structure of Clpnl2, as predicted by homology modeling, with those of other organisms. Both analyses revealed an early separation of bacterial pectin lyases from those found in fungi and oomycetes. Furthermore, two groups could be distinguished among the enzymes from fungi and oomycetes: one comprising enzymes from mostly saprophytic/opportunistic fungi and the other formed mainly by enzymes from pathogenic fungi and oomycetes. Clpnl2 was found in the latter group and was grouped together with the pectin lyase fromC. gloeosporioides.

Conclusions

TheClpnl2gene ofC. lindemuthianumshares the characteristic elements of genes coding for pectin lyases. A time-course analysis revealed significant differences between the two fungal races in terms of the expression ofClpnl2encoding for pectin lyase 2. According to the results, pectin lyases from bacteria and fungi separated early during evolution. Likewise, the enzymes from fungi and oomycetes diverged in accordance with their differing lifestyles. It is possible that the diversity and nature of the assimilatory carbon substrates processed by these organisms played a determinant role in this phenomenon.
Prevention of falls and fractures in old people by administration of calcium and vitamin d. randomized clinical trial
Authors: López-Torres Hidalgo Jesús ,   ANVITAD Group
Published: December 2011

Background

There are many studies that associate vitamin D serum levels in older persons with muscle strength, physical performance and risk of fractures and falls. However, current evidence is insufficient to make a general recommendation for administrating calcium and vitamin D to older persons. The objective of this study is to determine the effectiveness of calcium and vitamin D supplementation in improving musculoskeletal function and decreasing the number of falls in person aged over 65 years.

Methods/Design

Phase III, randomized, double blind, placebo-controlled trial to evaluate the efficacy of already marketed drugs in a new indication. It will be performed at Primary Care doctor visits at several Healthcare Centers in different Spanish Health Areas. A total of 704 non-institutionalized subjects aged 65 years or older will be studied (sample size calculated for a statistical power of 80%, alpha error 0.05, annual incidence of falls 30% and expected reduction of 30% to 20% and expected loss to follow up of 20%). The test drug containing 800 IU of vitamin D and 1000 mg of calcium will be administered daily. The control group will receive a placebo. The subjects will be followed up over two years. The primary variable will be the incidence of spontaneous falls. The secondary variables will include: consequences of the falls (fractures, need for hospitalization), change in calcidiol plasma levels and other analytical determinations (transaminases, PTH, calcium/phosphorous, albumin, creatinine, etc.), change in bone mass by densitometry, change in muscle strength in the dominant hand and change in musculoskeletal strength, risk factors for falls, treatment compliance, adverse effects and socio-demographic data.

Discussion

The following principles have been considered in the development of this Project: the product data are sufficient to ensure that the risks assumed by the study participants are acceptable, the study objectives will probably provide further knowledge on the problem studied and the available information justifies the performance of the study and its possible risk for the participants.

If calcium and vitamin D supplementation is effective in the prevention of falls and fractures in the elderly population, a recommendation may be issued with the aim of preventing some of the consequences of falls that affect quality of life and the ensuing personal, health and social costs.

Trial Registration

ClinicalTrials.gov: NCT01452243

Clinical trial authorized by the Spanish Medicines Agency: EudraCT number 2006-001643-63.
Effects of multi-walled carbon nanotubes (MWCNT) under Neisseria meningitidis transformation process
Authors: Mattos Ives B , Alves Danilo A , Hollanda Luciana M , Ceragiogli Helder J , Baranauskas Vitor , Lancellotti Marcelo
Published: December 2011

Background

This study aimed at verifying the action of multi-walled carbon nanotubes (MWCNT) under the naturally transformable Neisseria meningitidis against two different DNA obtained from isogenic mutants of this microorganism, an important pathogen implicated in the genetic horizontal transfer of DNA, causing the escape of the principal vaccination measured worldwide by the capsular switching process.

Materials and methods

The bacterium receptor strain C2135 was cultivated and had its mutant DNA donor M2 and M6, which received a receptor strain and MWCNT at three different concentrations. The inhibition effect of DNAse on the DNA in contact with nanoparticles was evaluated.

Results

The results indicated an in increase in the transformation capacity of N. meninigtidis in different concentrations of MWCNT when compared with negative control without nanotubes. A final analysis of the interaction between DNA and MWCNT was carried out using Raman Spectroscopy.

Conclusion

These increases in the transformation capacity mediated by MWCNT, in meningococci, indicate the interaction of these particles with the virulence acquisition of these bacteria, as well as with the increase in the vaccination escape process.
Primary clear cell sarcoma of humerus: case report
Authors: Liu Xudong , Zhang Huizhen , Dong Yang
Published: December 2011

We report a case of primary clear cell sarcoma occurring in the humerus. A 20 year old girl was referred to our hospital two years ago with painless swelling of the soft tissue surrounding the proximal right humerus. The conventional radiographic image showed an ill-defined, aggressive, osteolytic lesion associated with a partial cortical defect of the proximal right humerus. Magnetic resonance imaging found an irregularly shaped extraosseous mass extending from the proximal shaft of the right humerus, penetrating through the destroyed bone and invading the biceps brachii. After preoperative chemotherapy, a total tumor resection, internal fixation and bone cement implantation were performed. Histological studies of the resected tumor found that fibrous septa divided into well-defined nests and polygonal and fusiform cells with clear cytoplasm. Immunohistochemical studies demonstrated strong positive cytoplasmic staining with S-100 and scattered positivity with HMB-45. These findings led to a final, definitive diagnosis of clear cell sarcoma of the humerus. After routine postoperative chemotherapy, a 2-year follow-up showed no metastasis. Our findings in this report point out that primary clear cell sarcoma can originate from within the humerus and that limb salvage surgery can obtain a good result.
Integrity of chromatin and replicating DNA in nuclei released from fission yeast by semi-automated grinding in liquid nitrogen
Authors: Givens Robert M , Mesner Larry D , Hamlin Joyce L , Buck Michael J , Huberman Joel A
Published: December 2011

Background

Studies of nuclear function in many organisms, especially those with tough cell walls, are limited by lack of availability of simple, economical methods for large-scale preparation of clean, undamaged nuclei.

Findings

Here we present a useful method for nuclear isolation from the important model organism, the fission yeast, Schizosaccharomyces pombe. To preserve in vivo molecular configurations, we flash-froze the yeast cells in liquid nitrogen. Then we broke their tough cell walls, without damaging their nuclei, by grinding in a precision-controlled motorized mortar-and-pestle apparatus. The cryo-ground cells were resuspended and thawed in a buffer designed to preserve nuclear morphology, and the nuclei were enriched by differential centrifugation. The washed nuclei were free from contaminating nucleases and have proven well-suited as starting material for genome-wide chromatin analysis and for preparation of fragile DNA replication intermediates.

Conclusions

We have developed a simple, reproducible, economical procedure for large-scale preparation of endogenous-nuclease-free, morphologically intact nuclei from fission yeast. With appropriate modifications, this procedure may well prove useful for isolation of nuclei from other organisms with, or without, tough cell walls.
Mortality in people with depressive, anxiety and alcohol use disorders in Finland
Authors: Markkula Niina , Härkänen Tommi , Perälä Jonna , Partti Krista , Peña Sebastián , Koskinen Seppo , Lönnqvist Jouko , Suvisaari Jaana , Saarni Samuli I.
Published: December 2011

Background

Mental disorders are associated with increased mortality, but population-based surveys with reliable diagnostic procedures controlling for somatic health status are scarce.

Aims

To assess excess mortality associated with depressive, anxiety and alcohol use disorders and the principal causes of death.

Method

In a nationally representative sample of Finns aged 30–70 years, psychiatric disorders were diagnosed with the Composite International Diagnostic Interview. After an 8-year follow-up period, vital status and cause of death of each participant was obtained from national registers.

Results

After adjusting for sociodemographic factors, health status and smoking, depressive (hazard ratio (HR) = 1.97) and alcohol use disorders (HR = 1.72) were statistically significantly associated with mortality. Risk of unnatural death was increased among individuals diagnosed with anxiety disorders or alcohol dependence.

Conclusions

Individuals with depressive and alcohol use disorders have an increased mortality risk comparable with many chronic somatic conditions, that is only partly attributable to differences in sociodemographic, somatic health status and hazardous health behaviour.
THE BAG THAT SCOTT Bought: THE LOW VOWELS IN NORTHWEST WISCONSIN
Authors: Benson Erica J. , Fox Michael J. , Balkman Jared
Published: December 2011

Few studies have examined the vowel systems of communities that border areas characterized by the Northern Cities Shift and the low back vowel merger, two sound changes currently shaping North American English. Using data from 40 respondents in greater Eau Claire, in northwestern Wisconsin, we examine the behavior of the low vowels /æ, α, ɔ/ and consider the relationships among their arrangements and these sound changes. We find advanced /æ/-raising before /g/, moderate /æ/-raising in other environments, little to no /α/-fronting, and the low back vowel merger in progress. The findings challenge the assumption that /æ/-raising and the low back vowel merger are unlikely to co-occur. In addition, the patterns of /æ/-raising, for example, that /æ/-raising before /g/ is not undergoing change and /æ/-raising in other environments may be receding, raise interesting questions about the relationship between /æ/-raising in northwestern Wisconsin and the Northern Cities Shift. Although/æ/-raising seems to precede the low back vowel merger in northwestern Wisconsin, the pressure of the low back vowel merger is intensifying due to geographic and social connections between northwestern Wisconsin and minnesota.
WRN helicase regulates the ATR–CHK1-induced S-phase checkpoint pathway in response to topoisomerase-I–DNA covalent complexes
Authors: Patro Birija Sankar , Frøhlich Rikke , Bohr Vilhelm A. , Stevnsner Tinna
Published: December 2011

Checkpoints are cellular surveillance and signaling pathways that coordinate the response to DNA damage and replicative stress. Consequently, failure of cellular checkpoints increases susceptibility to DNA damage and can lead to profound genome instability. This study examines the role of a human RECQ helicase, WRN, in checkpoint activation in response to DNA damage. Mutations in WRN lead to genomic instability and the premature aging condition Werner syndrome. Here, the role of WRN in a DNA-damage-induced checkpoint was analyzed in U-2 OS (WRN wild type) and isogenic cells stably expressing WRN-targeted shRNA (WRN knockdown). The results of our studies suggest that WRN has a crucial role in inducing an S-phase checkpoint in cells exposed to the topoisomerase I inhibitor campthothecin (CPT), but not in cells exposed to hydroxyurea. Intriguingly, WRN decreases the rate of replication fork elongation, increases the accumulation of ssDNA and stimulates phosphorylation of CHK1, which releases CHK1 from chromatin in CPT-treated cells. Importantly, knockdown of WRN expression abolished or delayed all these processes in response to CPT. Together, our results strongly suggest an essential regulatory role for WRN in controlling the ATR–CHK1-mediated S-phase checkpoint in CPT-treated cells.
ARL4A acts with GCC185 to modulate Golgi complex organization
Authors: Lin Yu-Chun , Chiang Tsai-Chen , Liu Yu-Tsan , Tsai Yueh-Tso , Jang Li-Ting , Lee Fang-Jen S.
Published: December 2011

ADP-ribosylation factor-like protein 4A (ARL4A) is a developmentally regulated member of the ARF/ARL GTPase family. The primary structure of ARL4A is very similar to that of other ARF/ARL molecules, but its function remains unclear. The trans-Golgi network golgin GCC185 is required for maintenance of Golgi structure and distinct endosome-to-Golgi transport. We show here that GCC185 acts as a new effector for ARL4 to modulate Golgi organization. ARL4A directly interacts with GCC185 in a GTP-dependent manner. Sub-coiled-coil regions of the CC2 domain of GCC185 are required for the interaction between GCC185 and ARL4A. Depletion of ARL4A reproduces the GCC185-depleted phenotype, causing fragmentation of the Golgi compartment and defects in endosome-to-Golgi transport. GCC185 and ARL4A localize to the Golgi independently of each other. Deletion of the ARL4A-interacting region of GCC185 results in inability to maintain Golgi structure. Depletion of ARL4A impairs the interaction between GCC185 and cytoplasmic linker-associated proteins 1 and 2 (CLASP1 and CLASP2, hereafter CLASPs) in vivo, and abolishes the GCC185-mediated Golgi recruitment of these CLASPs, which is crucial for the maintenance of Golgi structure. In summary, we suggest that ARL4A alters the integrity of the Golgi structure by facilitating the interaction of GCC185 with CLASPs.
MT1-MMP regulates the turnover and endocytosis of extracellular matrix fibronectin
Authors: Shi Feng , Sottile Jane
Published: December 2011

The extracellular matrix (ECM) is dynamically remodeled by cells during development, normal tissue homeostasis and in a variety of disease processes. We previously showed that fibronectin is an important regulator of ECM remodeling. The deposition and/or polymerization of fibronectin into the ECM controls the deposition and stability of other ECM molecules. In addition, agents that inhibit fibronectin polymerization promote the turnover of fibronectin fibrils and enhance ECM fibronectin endocytosis and intracellular degradation. Endocytosis of ECM fibronectin is regulated by β1 integrins, including α5β1 integrin. We have examined the role of extracellular proteases in regulating ECM fibronectin turnover. Our data show that membrane type matrix metalloproteinase 1 (MT1-MMP; also known as MMP14) is a crucial regulator of fibronectin turnover. Cells lacking MT1-MMP show reduced turnover and endocytosis of ECM fibronectin. MT1-MMP regulates ECM fibronectin remodeling by promoting extracellular cleavage of fibronectin and by regulating α5β1-integrin endocytosis. Our data also show that fibronectin polymerization stabilizes fibronectin fibrils and inhibits ECM fibronectin endocytosis by inhibiting α5β1-integrin endocytosis. These data are the first to show that an ECM protein and its modifying enzyme can regulate integrin endocytosis. These data also show that integrin trafficking plays a major role in modulating ECM fibronectin remodeling. The dual dependence of ECM fibronectin turnover on extracellular proteolysis and endocytosis highlights the complex regulatory mechanisms that control ECM remodeling to ensure maintenance of proper tissue function.
Dermatan sulfotransferase Chst14/D4st1, but not chondroitin sulfotransferase Chst11/C4st1, regulates proliferation and neurogenesis of neural progenitor cells
Authors: Bian Shan , Akyüz Nuray , Bernreuther Christian , Loers Gabriele , Laczynska Ewa , Jakovcevski Igor , Schachner Melitta
Published: December 2011

Chondroitin sulfates (CSs) and dermatan sulfates (DSs) are enriched in the microenvironment of neural stem cells (NSCs) during development and in the adult neurogenic niche, and have been implicated in mechanisms governing neural precursor migration, proliferation and differentiation. In contrast to previous studies, in which a chondroitinaseABC-dependent unselective deglycosylation of both CSs and DSs was performed, we used chondroitin 4-O-sulfotransferase-1 (Chst11/C4st1)- and dermatan 4-O-sulfotransferase-1 (Chst14/D4st1)-deficient NSCs specific for CSs and DSs, respectively, to investigate the involvement of specific sulfation profiles of CS and DS chains, and thus the potentially distinct roles of CSs and DSs in NSC biology. In comparison to wild-type controls, deficiency for Chst14 resulted in decreased neurogenesis and diminished proliferation of NSCs accompanied by increased expression of GLAST and decreased expression of Mash-1, and an upregulation of the expression of the receptors for fibroblast growth factor-2 (FGF-2) and epidermal growth factor (EGF). By contrast, deficiency in Chst11 did not influence NSC proliferation, migration or differentiation. These observations indicate for the first time that CSs and DSs play distinct roles in the self-renewal and differentiation of NSCs.
Collagen V is a dominant regulator of collagen fibrillogenesis: dysfunctional regulation of structure and function in a corneal-stroma-specificCol5a1-null mouse model
Authors: Sun Mei , Chen Shoujun , Adams Sheila M. , Florer Jane B. , Liu Hongshan , Kao Winston W.-Y. , Wenstrup Richard J. , Birk David E.
Published: December 2011

Collagen V is a regulatory fibril-forming collagen that forms heterotypic fibrils with collagen I. Deletion of collagen V in the mouse is associated with a lack of fibril assembly in the embryonic mesenchyme, with a resultant lethal phenotype. The current work elucidates the regulatory roles of collagen V during development and growth of tissues. A conditional mouse model with a mutation in Col5a1 was developed using a Cre-loxP approach. Col5a1 was ablated in Col5a1flox/flox mice using a cornea stroma-specific Kera-Cre driver mouse to produce a bitransgenic Col5a1Δst/Δst line that is null for collagen V. This permits analyses of the corneal stroma, a widely used model for studies of collagen V. The collagen-V-knockout stroma demonstrated severe dysfunctional regulation of fibrillogenesis. Fibril diameters were significantly increased, with an abnormal, heterogeneous distribution; fibril structure was abnormal, fibril number was decreased and lamellae were disorganized with decreased stroma thickness. The phenotype was more severe in the anterior versus posterior stroma. Opacity was demonstrated throughout the Col5a1Δst/Δst stroma, with significantly increased haze intensity compared with control mice. These data indicate central regulatory roles for collagen V in fibril and matrix assembly during tissue development, with dysfunctional regulation resulting in a functional loss of transparency.