bluuebunny/crossref_metadata_2025 huggingface dataset:
Membrane Capacitive Deionization for Cooling Water Intake Reduction in Thermal Power Plants: Lab to Pilot Scale Evaluation
Authors: De Schepper Wim , Vanschepdael Christophe , Huynh Han , Helsen Joost
Published: March 2020

Cooling of thermal power stations requires large amounts of surface water and contributes to the increasing pressure on water resources. Water use efficiency of recirculating cooling towers (CT) is often kept low to prevent scaling. Partial desalination of CT feed water with membrane capacitive deionization (MDCI) can improve water quality but also results in additional water loss. A response surface methodology is presented in which optimal process conditions of the MCDI-CT system are determined in view of water use efficiency and cost. Maximal water use efficiency at minimal cost is found for high adsorption current (2.5 A) and short adsorption time (900 s). Estimated cost for MCDI to realize maximal MCDI-CT water use efficiency is relatively high (2.0–3.1 € m−3evap), which limits applicability to plants facing high intake water costs or water uptake limitations. MCDI-CT pilot tests show that water use efficiency strongly depends on CT operational pH. To allow comparison among pilot test runs, simulation software is used to recalculate CaCO3 scaling and acid dosage for equal operational pH. Comparison at equal pH shows that MCDI technology allows a clear reduction of CT water consumption (74%–80%) and acid dosage (63%–80%) at pH 8.5.
Moisture Influence on the Thermal Operation of the Late 19th Century Brick Facade, in a Historic Building in the City of Zamora
Authors: Cabeza-Prieto Alejandro , Camino-Olea María Soledad , Rodríguez-Esteban María Ascensión , Llorente-Álvarez Alfredo , Pérez María Paz Sáez
Published: March 2020

To improve the energy performance of restored cultural heritage buildings, it is necessary to know the real values of thermal conductivity of its envelope, mainly of the facades, and to study an intervention strategy that does not interfere with the preservation of their cultural and architectural values. The brick walls with which a large number of these buildings were constructed, usually absorb water, leading to their deterioration, whereas the heat transmission through them is much higher (than when they are dry). This aspect is often not taken into account when making interventions to improve the energy efficiency of these buildings, which makes them ineffective. This article presents the results of an investigation that analyzes thermal behavior buildings of the early 20th century in the city of Zamora, Spain. It has been concluded that avoiding moisture in brick walls not only prevents its deterioration but represents a significant energy saving, especially in buildings that have porous brick masonry walls and with significant thicknesses.
Tolerance-Insensitive Design of the Magnet Shape for a Surface Permanent Magnet Synchronous Motor
Authors: Lee Chung-Seong , Cha Kyoung-Soo , Park Jin-Cheol , Lim Myung-Seop
Published: March 2020

Many studies have been conducted to reduce the cogging torque of electric power steering motors. However, in the mass production of such motors, it is essential to enhance performance robustness in relation to tolerances. For such motors, this work analyzes performance robustness in relation to tolerances by applying a cycloid curve to the surface magnet of the rotor. Applying a cycloid curve to the magnet surface of the rotor is one of several ways to reduce cogging torque. To evaluate the performance of the cycloid curve, we compare it with an eccentric curve. The two curves are compared for the same specifications and evaluated using the indicator, tolerance insensitivity rate, which is used to assess performance robustness in relation to tolerances. The cycloid curve was evaluated to be more robust in relation to tolerances, as compared with the eccentric curve. Finally, an experiment was conducted to validate the robustness of the cycloid curve.
Designs of Feedback Controllers for Fluid Flows Based On Model Predictive Control and Regression Analysis
Authors: Sasaki Yasuo , Tsubakino Daisuke
Published: March 2020

Complexity of online computation is a drawback of model predictive control (MPC) when applied to the Navier–Stokes equations. To reduce the computational complexity, we propose a method to approximate the MPC with an explicit control law by using regression analysis. In this paper, we extracted two state-feedback control laws and two output-feedback control laws for flow around a cylinder as a benchmark. The state-feedback control laws that feed back different quantities to each other were extracted by ridge regression, and the two output-feedback control laws, whose measurement output is the surface pressure, were extracted by ridge regression and Gaussian process regression. In numerical simulations, the state-feedback control laws were able to suppress vortex shedding almost completely. While the output-feedback control laws could not suppress vortex shedding completely, they moderately improved the drag of the cylinder. Moreover, we confirmed that these control laws have some degree of robustness to the change in the Reynolds number. The computation times of the control input in all the extracted control laws were considerably shorter than that of the MPC.
Adaptive Multi-Model Switching Predictive Active Power Control Scheme for Wind Generator System
Authors: Li Hongwei , Ren Kaide , Li Shuaibing , Dong Haiying
Published: March 2020

To deal with the randomness and uncertainty of the wind power generation process, this paper proposes the use of the clustering method to complement the multi-model predictive control algorithm for active power control. Firstly, the fuzzy clustering algorithm is adopted to classify actual measured data; then, the forgetting factor recursive least square method is used to establish the multi-model of the system as the prediction model. Secondly, the model predictive controller is designed to use the measured wind speed as disturbance, the pitch angle as the control variable, and the active power as the output. Finally, the parameters and measured data of wind generators in operation in Western China are adopted for simulation and verification. Compared to the single model prediction control method, the adaptive multi-model predictive control method can yield a much higher prediction accuracy, which can significantly eliminate the instability in the process of wind power generation.
An Automatic UAV Based Segmentation Approach for Pruning Biomass Estimation in Irregularly Spaced Chestnut Orchards
Authors: Di Gennaro Salvatore Filippo , Nati Carla , Dainelli Riccardo , Pastonchi Laura , Berton Andrea , Toscano Piero , Matese Alessandro
Published: March 2020

The agricultural and forestry sector is constantly evolving, also through the increased use of precision technologies including Remote Sensing (RS). Remotely biomass estimation (WaSfM) in wood production forests is already debated in the literature, but there is a lack of knowledge in quantifying pruning residues from canopy management. The aim of the present study was to verify the reliability of RS techniques for the estimation of pruning biomass through differences in the volume of canopy trees and to evaluate the performance of an unsupervised segmentation methodology as a feasible tool for the analysis of large areas. Remote sensed data were acquired on four uneven-aged and irregularly spaced chestnut orchards in Central Italy by an Unmanned Aerial Vehicle (UAV) equipped with a multispectral camera. Chestnut geometric features were extracted using both supervised and unsupervised crown segmentation and then applying a double filtering process based on Canopy Height Model (CHM) and vegetation index threshold. The results show that UAV monitoring provides good performance in detecting biomass reduction after pruning, despite some differences between the trees’ geometric features. The proposed unsupervised methodology for tree detection and vegetation cover evaluation purposes showed good performance, with a low undetected tree percentage value (1.7%). Comparing crown projected volume reduction extracted by means of supervised and unsupervised approach, R2 ranged from 0.76 to 0.95 among all the sites. Finally, the validation step was assessed by evaluating correlations between measured and estimated pruning wood biomass (Wpw) for single and grouped sites (0.53 < R2 < 0.83). The method described in this work could provide effective strategic support for chestnut orchard management in line with a precision agriculture approach. In the context of the Circular Economy, a fast and cost-effective tool able to estimate the amounts of wastes available as by-products such as chestnut pruning residues can be included in an alternative and virtuous supply chain.
The Response of Erica arborea L. Tree Growth to Climate Variability at the Afro-alpine Tropical Highlands of North Ethiopia
Authors: Jacob Miro , De Ridder Maaike , Vandenabeele Marlies , Asfaha Tesfaalem , Nyssen Jan , Beeckman Hans
Published: March 2020

The important ecosystem services of the high altitude tropical afro-alpine Erica arborea L. forests are under increasing environmental and human pressure. The Erica treeline ecotone in the Ethiopian highlands forms a temperature-responsive vegetation boundary that is potentially affected by climate change. The cambium of 10 Erica arborea trees in Lib Amba Mountain and Ferrah Amba Mountain in the North Ethiopian highlands was marked in 2012, and corresponding tree disks were sampled after 498 days. Microphotographs of these cambial marks confirmed the formation of annual growth rings (0.76 ± 0.24 mm) with higher vessel density in earlywood and radially flattened fibers in the last layers of the latewood. In-continuum measurements of vessel size and density on microphotographs indicated the formation of inter-annual density fluctuations (IADFs) related to early rainfall in March-May. The same stem disks and 40 increment cores were used for detailed tree-ring analyses—a tree-ring chronology with 18 trees spanning from 1966 to 2014 could be derived. A significant (p < 0.1) positive correlation with minimum temperature in the growing season (August) and a negative correlation with minimum temperature in the spring season (March) were indicated as the most important climate factors regulating tree growth of Erica trees in the afro-alpine forest. The existence of annual tree rings and the proven potential for chronology building encourages further tree-ring analyses of Erica arborea in the afro-alpine tropical highlands in order to link it with climate variability and climate change.
Update of Genetic Linkage Map and QTL Analysis for Growth Traits in Eucommia ulmoides Oliver
Authors: Jin Cangfu , Li Zhouqi , Li Yu , Wang Shuhui , Li Long , Liu Minhao
Published: March 2020

Eucommia ulmoides (Tu-chung) is an economically and ecologically important tree species which has attracted worldwide attention due to its application in pharmacology, landscaping, wind sheltering and sand fixation. Molecular marker technologies can elucidate the genetic mechanism and substantially improve the breeding efficiency of E. ulmoides. The current research updated the original linkage map, and quantitative trait loci (QTL) analysis was performed on tree growth traits measured over 10 consecutive years in an E. ulmoides F1 population (“Xiaoye” × “Qinzhong No.1”). In total, 452 polymorphic markers were scored from 365 simple sequence repeat (SSR) primers, with an average of 1.24 polymorphic markers per primer combination. The integrated map was 1913.29 cM (centimorgan) long, covering 94.10% of the estimated genome and with an average marker density of 2.20 cM. A total of 869 markers were mapped into 19 major independent linkage groups. Growth-related traits measured over 10 consecutive years showed a significant correlation, and 89 hypothetical QTLs were forecasted and divided into 27 distinct loci. Three traits for tree height, ground diameter and crown diameter detected 25 QTLs (13 loci), 32 QTLs (17 loci) and 15 QTLs (10 loci), respectively. Based on BLASTX search results in the NCBI database, six candidate genes were obtained. It is important to explore the growth-related genetic mechanism and lay the foundation for the genetic improvement of E. ulmoides at the molecular level.
A Four-Year, Seven-State Reforestation Trial with Eastern Hemlocks (Tsuga canadensis) Resistant to Hemlock Woolly Adelgid (Adelges tsugae)
Authors: Kinahan Ian G. , Grandstaff Gabrielle , Russell Alana , Rigsby Chad M. , Casagrande Richard A. , Preisser Evan L.
Published: March 2020

We conducted over a decade of research into individual eastern hemlock (Tsuga canadensis; hemlock) trees that are potentially resistant to hemlock woolly adelgid (Adelges tsugae; HWA), an invasive xylem-feeding insect that is capable of rapidly killing even mature trees. Following clonal propagation of these individuals, in 2015 we planted size- and age-matched HWA-resistant and HWA-susceptible hemlocks in HWA-infested forest plots in seven states. In 2019, we re-surveyed the plots; 96% of HWA-resistant hemlocks survived compared to 48% of susceptible trees. The surviving HWA-resistant trees were also taller, produced more lateral growth, retained more foliage, and supported lower densities of the elongate hemlock scale Fiorinia externa, another invasive hemlock pest, than the surviving HWA-susceptible trees. Our results suggest that HWA management may benefit from additional research exploring the identification, characterization, and use of HWA-resistant eastern hemlocks in future reforestation efforts.
The Effect of a New Coating on the Drying Performance of Fruit and Vegetables Products: Experimental Investigation and Artificial Neural Network Modeling
Authors: Rahman S. M. Atiqure , Nassef Ahmed M. , Al-Dhaifallah Mujahed , Abdelkareem Mohammad Ali , Rezk Hegazy
Published: March 2020

A study on mass transfer using new coating materials (namely alginic acid and polygalacturonic acid) during osmotic dehydration—and hence in a laboratory-scale convective dryer to evaluate drying performance—was carried out. Potato and apple samples were examined as model heat-sensitive products in this study. Results indicate that the coating material containing both alginic acid and polygalacturonic acid causes higher water loss of about 17% and 7.5% and lower solid gain of about 4% and 8%, respectively, compared to uncoated potato sample after a typical 90 min osmotic dehydration process. Investigation of drying performance using both coating materials showed a higher reduction in the moisture content of about 22% and 18%, respectively, compared with uncoated samples after the 3 h drying period. Comparisons between the two proposed coating materials were also carried out. Samples (potato) coated with alginic acid demonstrated better performance in terms of higher water loss (WL), lower solid gain (SG), and notable enhancement of drying performance of about 7.5%, 8%, and 8%, respectively, compared to polygalacturonic acid. Similar outcomes were observed using apple samples. Additionally, an accurate model of the drying process based on the experimental dataset was created using an artificial neural network (ANN). The obtained mean square errors (MSEs) for the predicted water loss and solid gain outputs of the potato model were 4.0948e−5 and 3.924e−6, respectively. However, these values for the same parameters were 3.164e−5 and 4.4915e−6 for the apple model. The coefficient of determination (r2) values for the two outputs of the potato model were found to be 0.99969 and 0.99895, respectively, while they were 0.99982 and 0.99913 for the apple model, which reinforces the modeling phase.
Characterization of Defatted Products Obtained from the Parmigiano–Reggiano Manufacturing Chain: Determination of Peptides and Amino Acids Content and Study of the Digestibility and Bioactive Properties
Authors: Buhler Sofie , Riciputi Ylenia , Perretti Giuseppe , Caboni Maria Fiorenza , Dossena Arnaldo , Sforza Stefano , Tedeschi Tullia
Published: March 2020

Parmigiano–Reggiano (PR) is a worldwide known Italian, long ripened, hard cheese. Its inclusion in the list of cheeses bearing the protected designation of origin (PDO, EU regulation 510/2006) poses restrictions to its geographic area of production and its technological characteristics. To innovate the Parmigiano–Reggiano (PR) cheese manufacturing chain from the health and nutritional point of view, the output of defatted PR is addressed. Two defatting procedures (Soxhlet, and supercritical CO2 extraction) were tested, and the obtained products were compared in the composition of their nitrogen fraction, responsible for their nutritional, organoleptic, and bioactive functions. Free amino acids were quantified, and other nitrogen compounds (peptides, proteins, and non-proteolytic aminoacyl derivatives) were identified in the extracts and the mixtures obtained after simulated gastrointestinal digestion. Moreover, antioxidant and angiotensin converting enzyme (ACE) inhibition capacities of the digests were tested. Results obtained from the molecular and biofunctional characterization of the nitrogen fraction, show that both the defatted products keep the same nutritional properties of the whole cheese.
Antibiotics, Acid and Heat Tolerance of Honey adapted Escherichia coli, Salmonella Typhi and Klebsiella pneumoniae
Authors: Ayub Rabia , Umer Muhammad , Maan Abid Aslam , Rasool Bilal , Khan Muhammad Kashif Iqbal , Younis Tahira , Abbas Shabbar , Sajjad Muhammad , Kaleem Imdad , Imran Muhammad , Ullah Azmat , Afzal Muhammad Sohail , Shah Zaheer Hussain , Ahmed Sheraz , Aslam Farhan , Chaudhary Neelam , Afzal Muhammad Inam
Published: March 2020

The medicinal importance of honey has been known for many decades due to its antimicrobial properties against life-threatening bacteria. However, previous studies revealed that microorganisms are able to develop adaptations after continuous exposure to antimicrobial compounds. The present study was conducted to explore the impact of subinhibitory concentrations of branded honey (Marhaba) and unbranded honey (extracted from Ziziphus mauritiana plant) locally available in Pakistan on Escherichia coli ATCC 10536, Salmonella Typhi and Klebsiella pneumoniae by investigating the development of self- or cross-resistance to antibiotics (gentamicin, kanamycin and imipenem). Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of autoclaved honeys were determined. The bacterial cells of E. coli ATCC 10536, S. Typhi and K. pneumoniae were subjected to honey adaptation by exposing to ¼ × MIC (4 passages) and ½ × MIC (4 passages) of both honeys. Moreover, tolerance to low pH and high temperature was also studied in adapted and unadapted cells. The decreasing trend in growth pattern (OD600nm) of E. coli ATCC 10536, S. Typhi and K. pneumoniae was observed with increases in the concentration of honeys (6.25–50% v/v) respectively. Our results showed that continuous exposure of both honeys did not lead to the development of any self- or cross-resistance in tested bacteria. However, percent survival to low pH was found to be significantly higher in adapted cells as compared to unadapted cells. The results indicate that both branded honey (Marhaba) and unbranded honey (extracted from Ziziphus mauritiana plant) were effective in controlling the growth of tested pathogenic bacteria. However, the emergence of tolerance to adverse conditions (pH 2.5, temperature 60 °C) deserves further investigation before proposing honey as a better antibacterial agent in food fabrication/processing, where low pH and high temperatures are usually implemented.
The Effect of High-Intensity Ultrasound on the Physicochemical and Microbiological Properties of Mexican Panela Cheese
Authors: Carrillo-Lopez Luis M. , Juarez-Morales Monica G. , Garcia-Galicia Ivan A. , Alarcon-Rojo Alma D. , Huerta-Jimenez Mariana
Published: March 2020

High-intensity ultrasound could be an alternative to pasteurization for cheeses made with fresh raw milk, the properties of which must be preserved as part of their intangible cultural heritage, such as Panela cheese in Mexico. This research aimed to study the effect of the amplitude (50% and 100%) and application time (0, 5, and 10 min) of ultrasound treatment of fresh raw milk, on the yield and microbiological and physicochemical qualities of Panela cheese after 24 h of storage at 4 °C. The yield was increased to 24.29% with 10 min of ultrasonication, although the amount of exudate was higher in the ultrasonic product than in the control (20.33%). As the ultrasonication time increased, the yellowness (b*) increased significantly, while the hue angle decreased (with values close to 90°), resulting in evident yellow tones in cheeses made with milk treated for 10 min. The pH significantly increased from 6.6 to 6.74 with 5 min of ultrasound, but decreased to 6.37 with 10 min of ultrasonication. Although no significant differences were found in fat content, the protein significantly increased with 5 min of sonication, but it decreased markedly when ultrasound was applied for 10 min. Ultrasound treatment with amplitudes of 50% effectively decreased the counts of coliform bacteria regardless of ultrasonication time. However, the mesophilic bacteria increased by a 0.9 log with an amplitude of 100% and 10 min treatment. The results showed that ultrasound improved the yield and microbial, nutritional, and physicochemical properties of Panela cheese.
Proteins and Metabolites as Indicators of Flours Quality and Nutritional Properties of Two Durum Wheat Varieties Grown in Different Italian Locations
Authors: Graziano Sara , Marmiroli Nelson , Visioli Giovanna , Gullì Mariolina
Published: March 2020

Durum wheat is an important food source in Mediterranean countries, and Italy is the major producer of durum wheat in Europe. The quality of durum wheat flours depends on the type and amount of gluten proteins and starch while flour nutritional value rests on metabolite contents such as polyphenols. In this work, two Italian cultivars, Iride and Svevo, were analyzed for two years (2016–2017) in four Italian regions to explore how the environment affects: (i) reserve proteome; (ii) starch content and composition; and (iii) free, conjugated, bound phenolics and antioxidant capacity. The impact of environmental and meteorological conditions was significant for many traits. Regardless of the cultivation site, in 2017, a year with less rainfall and a higher temperature during grain filling, there was an increase in low molecular weight glutenins, in the glutenin/gliadin ratio and in the A-type starch granules size, all parameters of higher technological quality. In the same year, the cultivars showed higher amounts of polyphenols and antioxidant capacity. In conclusion, the two wheat cultivars, selected for their medium to high yield and their good quality, had higher performances in 2017 regardless of their sowing locations.
A Survey of Analytical Techniques for Noroviruses
Authors: Liu Lingling , Moore Matthew D.
Published: March 2020

As the leading cause of acute gastroenteritis worldwide, human noroviruses (HuNoVs) have caused around 685 million cases of infection and nearly $60 billion in losses every year. Despite their highly contagious nature, an effective vaccine for HuNoVs has yet to become commercially available. Therefore, rapid detection and subtyping of noroviruses is crucial for preventing viral spread. Over the past half century, there has been monumental progress in the development of techniques for the detection and analysis of noroviruses. However, currently no rapid, portable assays are available to detect and subtype infectious HuNoVs. The purpose of this review is to survey and present different analytical techniques for the detection and characterization of noroviruses.
Radical Scavenging and Antimicrobial Properties of Polyphenol Rich Waste Wood Extracts
Authors: Smailagić Anita , Ristivojević Petar , Dimkić Ivica , Pavlović Tamara , Dabić Zagorac Dragana , Veljović Sonja , Fotirić Akšić Milica , Meland Mekjell , Natić Maja
Published: March 2020

The main focus of this study is to assess radical scavenging and antimicrobial activities of the 11 wood extracts: oak (Quercus petraea (Matt.) Liebl., Q. robur L., and Q. cerris L.), mulberry (Morus alba L.), myrobalan plum (Prunus cerasifera Ehrh.), black locust (Robinia pseudoacacia L.), and wild cherry (Prunus avium L.). High-performance thin-layer chromatography (HPTLC) provided initial phenolic screening and revealed different chemical patterns among investigated wood extracts. To identify individual compounds with radical scavenging activity DPPH-HPTLC, assay was applied. Gallic acid, ferulic and/or caffeic acids were identified as the compounds with the highest contribution of total radical scavenging activity. Principal component analysis was applied on the data set obtained from HPTLC chromatogram to classify samples based on chemical fingerprints: Quercus spp. formed separate clusters from the other wood samples. The wood extracts were evaluated for their antimicrobial activity against eight representative human and opportunistic pathogens. The lowest minimum inhibitory concentration (MIC) was recorded against Staphylococcus aureus for black locust, cherry and mulberry wood extracts. This work provided simple, low-cost and high-throughput screening of phenolic compounds and assessments of the radical scavenging properties of selected individual metabolites from natural matrix that contributed to scavenge free radicals.
Enhancement of Agricultural Processed By-Products: Qualities Analysis of Fermentation Method in Gradient Salt Adding Treatment of Tuna Cooking Juice with Black Bean Koji Added
Authors: Ciou Jhih-Ying , Hsieh Lu-Sheng , Lee Tzu-Tai , Hsieh Chang-Wei
Published: March 2020

Fish sauce is popular for fermenting food in Southeast and Eastern Asia, while black bean is used to ferment condiments in Taiwan. Researchers have recently investigated the use of fish and black bean sauce in places where combining both fish and black bean is rare. This study was focused on fish sauce made from concentrated tuna cooking juice mixed with black bean koji. The experiment was divided into two stages. In the pre-fermentation stage, a suitable fermentation time with no salt added was determined. In the later fermentation stage, two preformatted samples of 4 and 7 days were added to salt water at 20 °Bé. In the pre-fermentation stage, the results show that the protease activity increased as time passed, but the pH value decreased. The highest browning degree was achieved after 120 days. In the later fermentation period, the total nitrogen contents for both experimental groups of 4 days and 7 days reached up to twice that of soy sauce. The total nitrogen content increased with time. In addition, the level of ammonia nitrogen increased from 0.08 to 0.15 g/dL in the first month. In conclusion, a new flavor of fermented sauce was produced in a shorter time and more effectively by combining tuna cooking juice and black bean.
Purification of Bioactive Peptide with Antimicrobial Properties Produced by Saccharomyces cerevisiae
Authors: Thyab Gddoa Al-sahlany Shayma , Altemimi Ammar , Al-Manhel Alaa , Niamah Alaa , Lakhssassi Naoufal , Ibrahim Salam
Published: March 2020

A variety of organisms produce bioactive peptides that express inhibition activity against other organisms. Saccharomyces cerevisiae is considered the best example of a unicellular organism that is useful for studying peptide production. In this study, an antibacterial peptide was produced and isolated from Saccharomyces cerevisiae (Baker’s yeast) by an ultrafiltration process (two membranes with cut-offs of 2 and 10 kDa) and purified using the ÄKTA Pure 25 system. Antibacterial peptide activity was characterized and examined against four bacterial strains including Gram-positive and Gram-negative bacteria. The optimum condition for yeast growth and antibacterial peptide production against both Escherichia. coli and Klebsiella aerogenes was 25–30 °C within a 48 h period. The isolated peptide had a molecular weight of 9770 Da, was thermostable at 50–90 °C for 30 min, and tolerated a pH range of 5–7 at 4 °C and 25 °C during the first 24 h, making this isolated antibacterial peptides suitable for use in sterilization and thermal processes, which are very important aspect in food production. The isolated antibacterial peptide caused a rapid and steady decline in the number of viable cells from 2 to 2.3 log units of gram-negative strains and from 1.5 to 1.8 log units of gram-positive strains during 24 h of incubation. The isolated antibacterial peptide from Saccharomyces cerevisiae may present a potential biopreservative compound in the food industry exhibiting inhibition activity against gram-negative and gram-positive bacteria.
RS Content and eGI Value of Cooked Noodles (I): Effect of Cooking Methods
Authors: Tian Yu , Li Ming , Tang Aoxing , Jane Jay-Lin , Dhital Sushil , Guo Boli
Published: March 2020

Noodles are widely consumed in China, which can be cooked in different ways. The effects of different cooking methods (boiling, steaming, microwave heating, stir-frying and frying) on the resistance starch (RS) content and digestive properties (digestion rate, digestibility and estimated glycemic index (eGI) value) of noodles were investigated. The RS content was greatly affected by the cooking time, and it was varied when the noodles were optimally cooked using different cooking methods. The RS contents of the microwaved and stir-fried noodles were relatively high (0.59%–0.99%), but it was lower (0.43%–0.44%) in the boiled and steamed noodles. Microwaved noodles showed the slowest digestion rate and the lowest eGI. Due to the limited water within fried noodles, none RS was found in the fried noodles, whereas stir-fried noodles showed RS5 formation from the XRD and DSC results. Compared with boiled and steamed noodles, the microwaved noodles showed a more compact morphology without porous holes on the surface, whereas fried noodles showed irregular morphology. The results indicated that the digestive properties of noodles made with the same ingredients can be greatly altered by using different cooking methods, and the digestive properties of different cooked noodles are worthy of confirmation using in vivo analysis.
Bioavailability of Melatonin from Lentil Sprouts and Its Role in the Plasmatic Antioxidant Status in Rats
Authors: Rebollo-Hernanz Miguel , Aguilera Yolanda , Herrera Teresa , Cayuelas L. Tábata , Dueñas Montserrat , Rodríguez-Rodríguez Pilar , Ramiro-Cortijo David , Arribas Silvia M. , Martín-Cabrejas María A.
Published: March 2020

Melatonin is a multifunctional antioxidant neurohormone found in plant foods such as lentil sprouts. We aim to evaluate the effect of lentil sprout intake on the plasmatic levels of melatonin and metabolically related compounds (plasmatic serotonin and urinary 6-sulfatoxymelatonin), total phenolic compounds, and plasmatic antioxidant status, and compare it with synthetic melatonin. The germination of lentils increases the content of melatonin. However, the phenolic content diminished due to the loss of phenolic acids and flavan-3-ols. The flavonol content remained unaltered, being the main phenolic family in lentil sprouts, primarily composed of kaempferol glycosides. Sprague Dawley rats were used to investigate the pharmacokinetic profile of melatonin after oral administration of a lentil sprout extract and to evaluate plasma and urine melatonin and related biomarkers and antioxidant capacity. Melatonin showed maximum concentration (45.4 pg/mL) 90 min after lentil sprout administration. The plasmatic melatonin levels increased after lentil sprout intake (70%, p < 0.05) with respect to the control, 1.2-fold more than after synthetic melatonin ingestion. These increments correlated with urinary 6-sulfatoxymelatonin content (p < 0.05), a key biomarker of plasmatic melatonin. Nonetheless, the phenolic compound content did not exhibit any significant variation. Plasmatic antioxidant status increased in the antioxidant capacity upon both lentil sprout and synthetic melatonin administration. For the first time, we investigated the bioavailability of melatonin from lentil sprouts and its role in plasmatic antioxidant status. We concluded that their intake could increase melatonin plasmatic concentration and attenuate plasmatic oxidative stress.
Modelling Growth and Decline in a Two-Species Model System: Pathogenic Escherichia coli O157:H7 and Psychrotrophic Spoilage Bacteria in Milk
Authors: Quinto Emiliano J. , Marín Juan M. , Caro Irma , Mateo Javier , Schaffner Donald W.
Published: March 2020

Shiga toxin-producing Escherichia coli O157:H7 is a food-borne pathogen and the major cause of hemorrhagic colitis. Pseudomonas is the genus most frequent psychrotrophic spoilage microorganisms present in milk. Two-species bacterial systems with E. coli O157:H7, non-pathogenic E. coli, and P. fluorescens in skimmed milk at 7, 13, 19, or 25 °C were studied. Bacterial interactions were modelled after applying a Bayesian approach. No direct correlation between P. fluorescens’s growth rate and its effect on the maximum population densities of E. coli species was found. The results show the complexity of the interactions between two species in a food model. The use of natural microbiota members to control foodborne pathogens could be useful to improve food safety during the processing and storage of refrigerated foods.
Comprehensive Analysis of Differentially Expressed Profiles of mRNA, lncRNA, and circRNA in the Uterus of Seasonal Reproduction Sheep
Authors: La Yongfu , He Xiaoyun , Zhang Liping , Di Ran , Wang Xiangyu , Gan Shangquan , Zhang Xiaosheng , Zhang Jinlong , Hu Wenping , Chu Mingxing
Published: March 2020

Photoperiod is one of the important factors leading to seasonal reproduction of sheep. However, the molecular mechanisms underlying the photoperiod regulation of seasonal reproduction remain poorly understood. In this study, we compared the expression profiles of mRNAs, lncRNAs, and circRNAs in uterine tissues from Sunite sheep during three different photoperiods, namely, the short photoperiod (SP), short transfer to long photoperiod (SLP), and long photoperiod (LP). The results showed that 298, 403, and 378 differentially expressed (DE) mRNAs, 171, 491, and 499 DE lncRNAs, and 124, 270, and 400 DE circRNAs were identified between SP and LP, between SP and SLP, and between LP and SLP, respectively. Furthermore, functional enrichment analysis showed that the differentially expressed RNAs were mainly involved in the GnRH signaling pathway, thyroid hormone synthesis, and thyroid hormone signaling pathway. In addition, co-expression networks of lncRNA–mRNA were constructed based on the correlation analysis between the differentially expressed RNAs. Our study provides new insights into the expression changes of RNAs in different photoperiods, which might contribute to understanding the molecular mechanisms of seasonal reproduction in sheep.
Phylogenetics of Taxus Using the Internal Transcribed Spacers of Nuclear Ribosomal DNA and Plastid trnL-F Regions
Authors: Coughlan Patricia , Carolan James , Hook Ingrid , Kilmartin Lisa , Hodkinson Trevor
Published: March 2020

Taxus is a genus of trees and shrubs with high value in horticulture and medicine as a source of the anticancer drug paclitaxel. The taxonomy of the group is complex due to the lack of diagnostic morphological characters and the high degree of similarity among species. Taxus has a wide global geographic distribution and some taxonomists recognize only a single species with geographically defined subgroups, whereas others have described several species. To address these differences in taxonomic circumscription, phylogenetic analyses were conducted on DNA sequences using Maximum Likelihood, Bayesian Inference and TCS haplotype networks on single and combined gene regions obtained for the nuclear ribosomal ITS region and the plastid trnL intron and trnL-F intergenic spacer. Evidence is presented for the sister group status of Pseudotaxus to Taxus and the inclusion of Amentotaxus, Austrotaxus, Cephalotaxus and Torreya within Taxaceae. Results are consistent with the taxonomic recognition of nine species: T. baccata, T. brevifolia, T. canadensis, T. cuspidata, T. floridana, T. fuana, T. globosa, T. sumatrana and T. wallichiana, but evidence is found for less species distinction and considerable reticulation within the T. baccata, T. canadensis and T. cuspidata group. We compare the results to known taxonomy, biogeography, present new leaf anatomical data and discuss the origins of the hybrids T. ×media and T. ×hunnewelliana.
Committed to Health: Key Factors to Improve Users’ Online Engagement through Facebook
Authors: Alonso-Cañadas Juana , Galán-Valdivieso Federico , Saraite-Sariene Laura , Caba-Pérez Carmen
Published: March 2020

Health organizations, continuously exposed to public scrutiny due to the social relevance of the services provided, have adopted social media to disseminate information about health but also about themselves, and thus, reducing uncertainty and improving communication. In this context, users’ participation in social media has become one of the main indicators of their effectiveness, highlighting the importance of analyzing which factors enhance online engagement. This research extends the number of variables identified in prior studies and analyzes 19,817 Facebook posts from 126 health organizations. Using multivariate linear regression, explanatory results show that economic and organizational attributes, and factors related to the social media posts, both contribute significantly to explain the engagement reached in social media by those organizations. According to our findings, health organizations are not taking enough advantage of social media to engage with their current and potential users. The dissemination of relevant information using visually attractive formats could help draw the attention of consumers, both to reach a higher commitment with the organization and to create value for society.
Mothers’ Healthcare Autonomy, Maternal-Health Utilization and Healthcare for Children under-3 Years: Analysis of the Nigeria DHS Data (2008–2018)
Authors: Ariyo Tolulope , Jiang Quanbao
Published: March 2020

This study was designed to simultaneously examine if mothers’ personal healthcare autonomy within the household, and the level of their maternal-healthcare utilization, translates into better preventive (complete immunization) and curative (treatments for diarrhoea, fever and acute respiratory infection) efforts on morbidities in child healthcare. We analysed data pooled from three consecutive waves of the Nigeria Demographic and Health Survey: the surveys of 2008, 2013 and 2018. Using a multilevel logistic regression, we estimated the odds ratio for each of the outcome variables while adjusting for covariates. Findings revealed that mothers’ health autonomy is positively associated with child immunization and treatment of morbidities (except diarrhoea), a relationship moderated by the frequency of mothers’ exposure to media. Additionally, mothers’ healthcare utilization is positively associated with complete immunization, and all forms of morbidity treatment (except diarrhoea). Although the relationship between mothers’ healthcare-utilization and child immunization is not dependent on family wealth, however, the relationship between mothers’ healthcare utilization and treatment of morbidity is dependent. Policy effort should be geared towards stimulating mothers to seek appropriate and timely child healthcare and future studies could consider looking into the mediating role of paternal support in this relationship.
Prevalence and Risk Factors of Metabolic Associated Fatty Liver Disease in Xinxiang, China
Authors: Li Hongbin , Guo Meihao , An Zhen , Meng Jun , Jiang Jing , Song Jie , Wu Weidong
Published: March 2020

Metabolic associated fatty liver disease (MAFLD) is recognized as the liver disease component of metabolic syndrome, which is mainly related to insulin resistance and genetic susceptibility. It is the most prevalent chronic liver disease worldwide. With rapid lifestyle transitions, its prevalence worldwide is increasing, and tremendous challenges in controlling this pandemic are arising. The objective of this study was to investigate the prevalence and risk factors of MAFLD in rural areas of Xinxiang, Henan in 2017. We conducted a cross-sectional analysis of rural inhabitants aged 20–79 years in Xinxiang, Henan in 2017, using cluster random sampling (N = 9140). Physical examinations were conducted at local clinics from April to June 2017. After overnight fasting, all participants underwent physical examinations, blood routine tests, biochemical examinations, and liver ultrasound and completed questionnaires. We investigated the crude and age-adjusted MAFLD prevalence and analyzed the characteristics of those with, and without, MAFLD, using logistic regression. Approximately 2868 (31.38%) participants were diagnosed with MAFLD. The overall age-adjusted MAFLD prevalence was 29.85% (men: 35.36%; women: 26.49%). The MAFLD prevalence increased with age, and peaked at the 50–59-year age group, and then began to decline. Higher body mass index, waist circumference, percentage of lymphocytes, levels of hemoglobin, platelet count, triglyceride, fasting plasma glucose, and serum uric acid were independently and positively correlated with MAFLD; In contrary, active physical activity and high-density lipoprotein cholesterol were negatively correlated with MAFLD. In summary, the MAFLD prevalence in the study population was 29.85%. Higher body mass index, waist circumference, percentage of lymphocytes, levels of hemoglobin, platelet count, triglyceride, fasting plasma glucose, and serum uric acid were risk factors for MAFLD.
What Drives the Performance of Collaboration Networks: A Qualitative Comparative Analysis of Local Water Governance in China
Authors: Cui Can , Yi Hongtao
Published: March 2020

Local water governance is challenging given the significance to public health and the difficulties to manage it in a fragmented administrative system. A collaboration network is a popular governance tool in local governance to cope with functional fragmentation problems and institutional collective action (ICA) dilemmas. Empirical works are needed to examine the outcomes of such governance networks, especially in the context of environmental governance. With fuzzy-set qualitative comparative analysis (fsQCA), this article seeks to evaluate the outcomes of collaboration networks by investigating the influence of network structures on local water governance performance in China. Based on empirical analyses on a dataset of twenty local water governance networks implementing the Water Ecological Civilization Pilot Project in China, the findings suggest that a high overall bridging and bonding of social capital and a low network density are important determinants of effective collaboration networks. This study has policy implications for the design of local collaboration networks in facilitating effective environmental governance.
Analysis and Optimization of Mn Removal from Contaminated Solid Matrixes by Electrokinetic Remediation
Authors: Cameselle Claudio , Gouveia Susana , Cabo Adrian
Published: March 2020

Electrokinetic remediation is a useful technique for the removal of ionic contaminants in soils, sediments, sludges, and other solid porous matrixes. The efficiency of metal removal and the electricity consumption in the electrokinetic treatment of soils largely depend on electric and physicochemical conditions. This study analyzes the electrokinetic treatment of Mn contaminated kaolinite clay specimen and the influence of voltage, current intensity, moisture content, pH, and facilitating agents on metal removal and energy consumption. The objective of this study is to identify the influence of the typical variables used in electrokinetic remediation. The results showed that the operation at constant voltage or constant current intensity were equivalent in terms of metal removal and energy consumption, as long as the electric field intensity was kept low to minimize the consumption in parallel electrochemical reactions, especially the electrolysis of water. The moisture content had a significant influence on the Mn removal. Moisture content higher that 50 percent resulted in very effective Mn removal as compared with kaolinite specimens with lower moisture. The control of pH in the electrolyte solutions and the addition of facilitating agents (organic acids) enhanced the removal of Mn but increased the electric energy cost. Overall, the best conditions for Mn removal involved low to moderate electric potential difference (10 to 30 V), the use of citric acid as the facilitating agent, and the pH control in the cathode at a slightly acid pH. The electrokinetic treatment of a sludge from a water treatment plant contaminated with Mn was effective when pH control on the cathode was used. Mn and various metals (66% of Mn, 30% of Cu, 56% of Zn, 21% Sr, and 21% of Fe) were removed with moderate electricity and acid consumption.
Association of Ambient Air Pollution with Nasopharyngeal Carcinoma Incidence in Ten Large Chinese Cities, 2006–2013
Authors: Yang Tianan , Liu Yexin , Zhao Weigang , Chen Zhenjiao , Deng Jianwei
Published: March 2020

Large cities in China are experiencing severe ambient air pollution. Although China accounts for more than 45% of new cases of nasopharyngeal carcinoma worldwide in 2018, few studies have examined the association between ambient air pollution and the high nasopharyngeal carcinoma (NPC) incidence in China. Thus, we aim to investigate whether exposure to ambient air pollution (including nitrogen dioxide, sulfur dioxide, and PM10) would significantly affect NPC incidence in large Chinese cities. We collected panel data of ten Chinese provincial cities about local NPC incidence, air pollution level, meteorology, and city profiles during 2006 to 2013 to construct a two-way fixed-effects model to explore the association between ambient air pollution and NPC incidence, as well as possible regional and gender differences behind the association. We found that NO2 had the strongest association with NPC incidence, and the relative risks were 2.2995 (95% CI, 1.2567–4.2075) for males and 1.3010 (95% CI, 0.8212–2.0620) for females, respectively. Under cumulative exposure, it was still NO2 that had the strongest association with NPC incidence, with a relative risk of 1.8836 (95% CI, 1.2416–2.8577), compared to 1.0857 (95% CI, 0.9474–1.2450) and 1.0547 (95% CI, 0.8790–1.2663) for SO2 and PM10, respectively. In addition, males were found more sensitive to ambient air pollution than females. We also found that southern Chinese cities were more sensitive to NO2 than northern cities, which might be related to a higher humidity there. Our study reveals that exposure to ambient air pollutants like SO2, PM10, and particularly NO2, is significantly positively associated with NPC incidence in China.