bluuebunny/crossref_metadata_2025 huggingface dataset:
Male and overconfident groups overinvest due to inflated perceived ability to beat the odds
Authors: Renerte Baiba , Hausfeld Jan , Twardawski Torsten
Published: March 2023

Organizational decisions are often made by groups rather than individuals. Depending on the group composition, each member's characteristics—like gender and motivated beliefs—can influence the final group investment decision. To capture this, we design two types of investment situations in a randomized controlled laboratory experiment—one with fixed chances of success and one with performance-dependent chances of success. This novel design entails the perceived ability to “beat the odds” of the investment and thus models real-life investment situations more accurately than standard lottery choice. Our results demonstrate the benefits of mixed group composition in terms of both gender and overconfidence: Groups with all men and/or all overconfident group members consistently overinvest when a possibility to “beat the odds” is present, but not in standard situations. We explore several channels for our results and find that (i) individual probability perception, (ii) leader responsibility allocation and (iii) spillover effects from priming show significant effects.
Building a responsible innovation toolkit as project legacy
Authors: Stahl Bernd Carsten , Bitsch Lise
Published: March 2023

This article explores whether and in what way it is possible to employ toolkits for responsible research and innovation (RRI toolkits) as mechanisms for ensuring the legacy of RRI in research projects. Based on a review of the concept of responsible research and innovation as well as existing toolkits in the area, the article offers an account of the development of an RRI toolkit in the context of the EU- funded Human Brain Project. This toolkit is designed to integrate insights and practices of responsible research and innovation developed over a 10 year period into the project legacy, the EBRAINS research infrastructure. The article suggests that toolkits have the potential to contribute to ensuring a long- lasting legacy of work undertaken in responsible research and innovation, but that this potential requires further support from institutions and the broader research environment to become realized.
Adverse health events and recommended health research priorities in agility dogs as reported by dog owners
Authors: Sellon Debra C. , Marcellin-Little Denis J. , McFarlane Dianne , McCue Molly , Pechette Markley Arielle , Shoben Abigail
Published: March 2023

Objective

To understand relative frequency of adverse health events, defined as injuries or infectious diseases, in dogs participating in agility and to determine health research priorities of agility dog owners.

Procedures

An internet-based questionnaire distributed to agility dog owners included items related to experiences with infectious diseases and injuries in agility dogs, reasons for retirement of dogs from competition, and ranking of health research priorities. Frequencies of infectious diseases in US geographic regions were compared with Chi-square tests. Research priority rankings were determined as median and interquartile range (IQR) for each topic. Rank-based tests (Kruskal Wallis and Mann-Whitney) compared rankings between participants in different agility organizations, between veterinarian and non-veterinarian competitors, and between respondents who had competed in national championship events and other respondents.

Results

There were 1,322 respondents who had competed in canine agility in the previous 6 months, with those respondents reporting a median time competing in the sport of 13 years (IQR = 8–20 years); 50% of respondents had competed in at least one national championship agility event in the preceding 5 years. Overall, 1,015 respondents (77%) indicated that one or more of their dogs had been injured and approximately one-third (n = 477, 36%) indicated that one or more dogs had likely acquired one or more infectious diseases as a result of agility activities. Specific types of infectious diseases acquired varied by geographic region in the US. Research priority rankings were similar regardless of preferred agility organization or respondent experience. The highest-ranking research topics were identification of risk factors for specific types of injuries, improvements in equipment and understanding of safe course design, and physical conditioning programs to prevent injury.

Conclusions and clinical relevance

Agility competitors prioritize research in areas that advance understanding of injury prevention in their dogs. Research priorities are nearly uniform among competitors regardless of their preferred agility organization or level of experience, providing a strong rationale for agility organizations to collaborate in research initiatives that improve safety and well-being for dogs competing in the sport. There has been little published research focusing on the high-priority research areas identified by competitors.
A Duality Model of Dynamic Capabilities: Combining Routines and Improvisation
Authors: Carvalho Ana
Published: March 2023

Dynamic Capabilities are predominantly conceptualised as being based on routine, stable patterns of behaviour. This is paradoxical with their intended purpose to elicit change in firms’ resource base and operational capabilities that allows them to face highly dynamic environments. I contribute to resolve this paradox by bringing the concept of duality to the Dynamic Capabilities debate. In particular, I argue that the view of Dynamic Capabilities as routines stems from the misconception that reliable outcomes can be attained only through stable mechanisms. Drawing on the literature on routine dynamics and on improvisation, as well as the empirical evidence of previous research, I propose a duality model that conceives Dynamic Capabilities as based both on routines and on improvisation. This duality perspective highlights the interdependence and complementarity between predictable stable routines and extemporaneous improvised actions that are both necessary to reliably achieve the (often radical) change needed to compete in highly dynamic environments. Rather than dealing with stable routines and improvisation as a trade-off, organisations must dynamically strive for the right balance, managing them concurrently and synergistically.
Assessment of Aircraft Coating Resistance to Lighting Strikes and Long-Term Environmental Impact
Authors: Pecho Pavol , Veľký Patrik , Bugaj Martin , Kajánek Daniel
Published: March 2023

During an aircraft’s flight through the atmosphere, the aircraft structure interacts with the surrounding environment. One of the potential hazards that can occur is a lightning strike to the structure covering. Subsequent damage to the aircraft covering may cause safety risks, so it must be removed. This paper describes a method of producing an electrical discharge that simulates the effect of lightning on metallic and non-metallic types of aircraft coverings upon which various types of paint coatings are applied. The electrical discharge hits the coating samples and, based on the material and type of paint used, causes damage. The coatings were later tested in a salt chamber according to STN ISO 9227:2017, and were photodocumented at various time intervals and subsequently analysed. The salt chamber test exposes the material to adverse conditions, which, in relation to the damaged coating, cause corrosion of the material, which depends on the extent of the damage and the type of material. The paper concludes that metallic aircraft coating samples allow less damage to aircraft coverings after a lightning strike, which is linked with less required maintenance associated with financial and time costs.
Multi-Objective, Multi-Disciplinary Design Optimization and Multi-Attribute Evaluation of Hybrid Rocket Motors Used for Manned Lunar Lander
Authors: Liu Yang , Li Xintong , Wang Pengcheng , Zhang Xiaotian , Zhu Hao , Cai Guobiao
Published: March 2023

This paper proposed a multi-objective, multi-disciplinary design optimization and multi-attribute evaluation method for the manned lunar lander descent stage. A system design model is established considering multiple disciplines such as propulsion, structure, trajectory and cost. With the goal of minimizing mass, minimizing cost and maximizing velocity increment, the overall scheme for hybrid rocket motors was optimized by altering various grain shapes and feed systems, acting as an alternative propulsion scheme for the manned lunar lander. Under the same design conditions, the optimal scheme of hybrid rocket motors considering continuous and discrete attributes was studied and compared with that of liquid rocket engines to elucidate the characteristics of the hybrid rocket motors for deep space exploration. The results showed that the evaluation results obtained by considering only continuous attributes were different from those by considering both continuous and discrete attributes. The liquid propulsion scheme with liquid oxygen/kerosene is superior to the hybrid propulsion schemes due to its excellent cost and specific impulse performance when only continuous attributes are considered. However, hybrid rocket motors have shown good performance in terms of operability, manufacturability, safety and environmental protection. So, after introducing discrete attributes, the hybrid propulsion schemes show greater potential. In brief, based on the multi-attribute evaluation method considering comprehensive attributes, the hybrid rocket motor provided with tube grain and gas pressure feed system was considered as the optimal propulsion system for the lunar lander. Furthermore, the parametric analysis showed that the fuel grain outside diameter, the initial design thrust and the initial oxygen-to-fuel ratio had a significant influence on the performance of the hybrid rocket motor for the lunar lander, and in particular, the effect of the fuel grain outside diameter was more than 50%.
Three-Dimensional Shock Topology Detection Method via Tomographic Reconstruction
Authors: Lin Mengnan , Tian Zhongwei , Chang Siyuan , Cui Kai , Dai Shulan
Published: March 2023

Shock waves and shock-shock interaction are typical phenomena in supersonic or hypersonic flows that have significant impacts on aerodynamic performance. To obtain a comprehensive understanding of the mechanism of shock wave interaction, shock wave detection (SWD) methods are required. However, it is often challenging for most current SWD methods to identify the relationship between shock waves (also known as shock topology). To address this issue, this paper proposes a novel three-dimensional shock topology detection method based on the tomographic reconstruction strategy. This method involves extracting parallel slices from the flow field, then utilizing a two-dimensional shock topology recognition algorithm to obtain shock lines. Shock bands are obtained by connecting shock lines for every two adjacent slices, and shock surfaces are generated by assembling shock bands. Interaction lines are also formed by connecting interaction points. The detected shock wave is a structure composed of “point-line-band-surface”, and the topology relationship with other shock waves is obvious. Numerical results show that the shock waves detected by the proposed method can be categorized into families. Moreover, the shock surfaces generated by this method are free of gaps, holes, and un-physical fragments, which is an improvement over existing SWD methods.
Improvement of Take-Off Performance for an Electric Commuter Aircraft Due to Distributed Electric Propulsion
Authors: Cusati Vincenzo , Corcione Salvatore , Nicolosi Fabrizio , Zhang Qinyin
Published: March 2023

The need for environmentally responsible solutions in aircraft technology is now considered the priority for global challenges related to the limited supply of traditional fuel sources and the potential global hazards associated with emissions produced by traditional aircraft propulsion systems. Several projects, including research into highly advanced subsonic aircraft concepts to drastically reduce energy or fuel usage, community noise, and emissions associated with aviation, are currently ongoing. One of the proposed propulsion concepts that address European environmental goals is distributed electric propulsion. This paper deals with the detailed aerodynamic analyses of a full-electric commuter aircraft with fuel cells, which expects two primary electric motors at the wing tip and eight other electric motors distributed along the wingspan as secondary power sources. The main objective was the numerical estimation of propulsive effects in terms of lift capabilities at take-off conditions to quantify the possible reduction of take-off field length. However, the aircraft was designed from scratch, and therefore a great effort was spent to design both propellers (for the tip and distributed electric motors) and the wing flap. In this respect, several numerical tests were performed to obtain one of the best possible flap positions. This research work estimated a reduction of about 14% of the take-off field length due to only the propulsive effects. A greater reduction of up to 27%, if compared to a reference conventional commuter aircraft, could be achieved thanks to a combined effect of distributed propulsion and a refined design of the Fowler flap. On the contrary, a significant increment of pitching moment was found due to distributed propulsion that may have a non-negligible impact on the aircraft stability, control, and trim drag.
Adaptive Division and Analysis Data Extraction of Air-Cooled Turbine Blade for Pipe Network Calculation
Authors: Wang Tian , Liu Zhenbo , Li Jixing , Liu Yu , Ma Xingyu , Yang Jiong
Published: March 2023

Manually preparing the data for the analysis of the calculation of a pipe network of air-cooled turbine blades is inefficient. In this paper, a method to adaptively divide the blade model and extract data of the flow units is proposed. In this method, the topological lines of the flow channels for the calculations of the pipe network were extracted based on the simulatedfluid flow. The abstracted lines of the fluid flow area were used to replace the manual interpretation of expertsto realize the adaptive division of the flow units and to understand the connection relationship of the units and the layout of the spatial network. In addition, by establishing a basic flow unit library and constructing a specific algorithm, we quickly extracted the data from the typical flow units. Finally, the proposed method and algorithm were verified with a representative case study. The results illustrated that our method could cut down the pre-processing in the calculation of the pipe network of air-cooled turbine blades to several minutes from the several hours required for the manual method while providing reliable quality.
Use of Computational Fluid Dynamics to Study Ammonia Concentrations at Pedestrian Height in Smart Broiler Chamber Clusters
Authors: Li Mengxi , Zou Xiuguo , Feng Bo , Qiu Xinfa
Published: March 2023

NH3 emissions are an environmental issue that is of wide concern in livestock production. In intensive livestock farming, it is necessary to study outdoor ammonia concentrations under various conditions to maximize the protection of livestock caretakers’ health in and around the facilities. In this study, the ammonia concentrations outside smart broiler chambers in 60 scenarios, with conditions including 4 broiler chamber densities, 3 wind directions, and 5 outlet emission intensities, were simulated based on computational fluid dynamics (CFD) technology. The results show that (1) outdoor ammonia tends to accumulate near the outlet when the wind direction angle is small, while it has a wider range of influence when the angle is vertical; (2) building a smart broiler chamber cluster for intensive livestock farming is environmentally friendly; and (3) keeping the ammonia outlet perpendicular to the local dominant wind direction can effectively prevent high concentrations of ammonia around the chambers. In practical applications, the conclusions of this study can be used to arrange the layout and direction of smart broiler chamber clusters.
Real-Time Kinematic Imagery-Based Automated Levelness Assessment System for Land Leveling
Authors: Guan Senlin , Takahashi Kimiyasu , Nakano Keiko , Fukami Koichiro , Cho Wonjae
Published: March 2023

Many cropping systems, notably for rice or soybean production, rely largely on arable land levelness. In this study, an automated levelness assessment system (ALAS) for evaluating lowland levelness is proposed. The measurement accuracy of total station, real-time kinematic (RTK) receiver, and RTK unmanned aerial vehicle (UAV) instruments used at three study sites was evaluated. The ALAS for assessing the levelness of agricultural lowlands (rice paddy fields) was then demonstrated using UAV-based imagery paired with RTK geographical data. The ALAS (also a program) enabled the generation of an orthomosaic map from a set of RTK images, the extraction of an orthomosaic map of a user-defined field, and the visualization of the ground altitude surface with contours and grade colors. Finally, the output results were obtained to assess land levelness before and after leveling. The measurement accuracy results of the instruments used indicated that the average horizontal distance difference between RTK-UAV and total station was 3.6 cm, with a standard deviation of 1.7 cm and an altitude root mean squared error of 3.3 cm. A visualized ground altitude surface and associated altitude histogram provided valuable guidance for land leveling with the ALAS; the ratios of the ground altitude of ±5 cm in the experiment fields (F1 and F2) increased from 78.6% to 98.6% and from 71.0% to 96.9%, respectively, making the fields more suitable for rice production. Overall, this study demonstrates that ALAS is promising for land leveling and effective for further use cases such as prescription mapping.
Prediction of Pea (Pisum sativum L.) Seeds Yield Using Artificial Neural Networks
Authors: Hara Patryk , Piekutowska Magdalena , Niedbała Gniewko
Published: March 2023

A sufficiently early and accurate prediction can help to steer crop yields more consciously, resulting in food security, especially with an expanding world population. Additionally, prediction related to the possibility of reducing agricultural chemistry is very important in an era of climate change. This study analyzes the performance of pea (Pisum sativum L.) seed yield prediction by a linear (MLR) and non-linear (ANN) model. The study used meteorological, agronomic and phytophysical data from 2016–2020. The neural model (N2) generated highly accurate predictions of pea seed yield—the correlation coefficient was 0.936, and the RMS and MAPE errors were 0.443 and 7.976, respectively. The model significantly outperformed the multiple linear regression model (RS2), which had an RMS error of 6.401 and an MAPE error of 148.585. The sensitivity analysis carried out for the neural network showed that the characteristics with the greatest influence on the yield of pea seeds were the date of onset of maturity, the date of harvest, the total amount of rainfall and the mean air temperature.
Fine-Mapping Analysis of the Genes Associated with Pre-Harvest Sprouting Tolerance in Rice (Oryza sativa L.)
Authors: Jang Seong-Gyu , Kim Backki , Choi Insoo , Lee Joohyun , Ham Tae-Ho , Kwon Soon-Wook
Published: March 2023

Pre-harvest sprouting (PHS) of rice (Oryza sativa L.) causes severe economic problems due to reduced grain quality and yield. Fine mapping was carried out to identify genes associated with PHS; the detected quantitative trait locus (QTL) was narrowed down to 50 Kbp using F3:4 populations, four polymorphic insertion and deletion (InDel) markers, and two cleaved amplified polymorphic sequence (CAPS) markers. In one region, five candidate genes were detected, and the SNP and InDel in each gene (Os01g0111400 and Os01g0111600) were confirmed to show the differences and resulting amino acid changes between parent plants. Based on haplotype, expression, and co-segregation analysis, the InDel in Os01g0111600 was confirmed to be associated with the PHS trait. The results of this study could be applied to improve the PHS tolerance of Japonica rice varieties, and they also improved our understanding of the genetic basis underlying PHS tolerance.
Multiplex PCR for Discriminating Host Plant Associations of Hyalesthes obsoletus (Hemiptera: Cixiidae), a Key Vector and Driver of ‘Ca. Phytoplasma solani’ Epidemiology
Authors: Jović Jelena , Toševski Ivo
Published: March 2023

Given the ecological and epidemiological specialization of Hyalesthes obsoletus, the principle vector of ‘Candidatus Phytoplasma solani’, the primary objective of this study was to develop molecular tools for discriminating three host plant associations of the vector populations: (i) Convolvulus arvensis–Urtica dioica (Ca–Ud), (ii) Vitex agnus-castus (Vac), and (iii) Crepis foetida (Cf). The genetic diversity of the nearly full-length mitochondrial COI gene (1467 bp) was analyzed and compared among previously reported and newly collected individuals of the three host plant associations on a wide geographic range. Multiplex PCR was designed and evaluated for discriminating H. obsoletus host plant associations based on the size of amplified fragments: 1084 bp for the Cf association, 645 bp for the Ca–Ud association, and 355 bp for the Vac association. Examples of the epidemiological value of combining data on the genetic characteristics of the vector and the pathogen are provided. The method is intended to facilitate an accurate identification of the vector’s phylogenetic lineage, natural host plant preference, and epidemiological transmission routes of ‘Ca. P. solani’. When applied to H. obsoletus specimens collected from cultivated plants within an agroecosystem and combined with ‘Ca. P. solani’ genotyping, the method should provide valuable information on disease epidemiology, source(s) of emergence, and transmission routes.
Development of a Multiplex RT-PCR Assay for Simultaneous Detection of Ten Major Viral Pathogens of Wheat
Authors: Deb Mahua , Anderson Joseph M. , Scofield Steven R.
Published: March 2023

Triticum mosaic virus (TriMV) and High plains virus (HPV), identified recently, have been considered among the major viruses that affect wheat. Carried by the same vector, wheat curl mite, both of these viruses cause yellowing and stunting of plants which are very similar to many other viruses attacking wheat. This makes it difficult to detect these viruses in different wheat lines, posing a major problem in the yield. This paper highlights the addition of these two viruses to a multiplex RT-PCR based method which already detected the presence of barley and cereal yellow dwarf viruses (B/CYDVs), soil-borne wheat mosaic virus (SBWMV), wheat spindle streak mosaic virus (WSSMV), and wheat streak mosaic virus (WSMV). The method uses specific sets of primers that detect the target viruses TriMV and HPV at 560 bp and 490 bp, respectively, in the presence of other distinct viruses such as B/CYDVs -PAV, -MAV, -SGV, -RPV, -RMV, WSSMV, SBWMV, and WSMV at 295, 175, 237, 400, 365, 154, 219, and 193 bp, respectively. The forward primer for each specific virus was fluorescently tagged to detect it in a higher throughput manner in capillary electrophoresis. All ten viruses may be viewed as peaks in an electropherogram from the capillary electrophoresis corresponding to their product sizes in base pairs. This advancement in the protocol allows detection of all ten wheat viruses in a single test, thus improving the diagnostic capability with only a slight increase in cost.
Assessing Drought Tolerance of Newly Developed Tissue-Cultured Canola Genotypes under Varying Irrigation Regimes
Authors: Morsi Nahid A. A. , Hashem Omnia S. M. , El-Hady Mohamed A. Abd , Abd-Elkrem Yasser M. , El-temsah Mohamed E. , Galal Elhussin G. , Gad Khaled I. , Boudiar Ridha , Silvar Cristina , El-Hendawy Salah , Mansour Elsayed , Abdelkader Mohamed A.
Published: March 2023

Drought is a major abiotic stress that greatly affects canola growth, production, and quality. Moreover, water scarcity is projected to be more severe and frequent as a result of climate change, in particular in arid environments. Thereupon, developing drought-tolerant and high-yielding canola genotypes has become more critical to sustaining its production and ensuring global food security with the continuing population growth. In the present study, ten canola genotypes comprising six developed tissue-cultured canola genotypes, two exotic genotypes, and two commercial cultivars were evaluated under four irrigation regimes. The applied irrigation regimes were well-watered (100% crop evapotranspiration, ETc), mild drought (80% ETc), moderate drought (60% ETc), and severe drought (40% ETc) conditions. Drought-stress treatments (80, 60, and 40% ETc) gradually reduced the chlorophyll content, relative water content, flowering time, days to maturity, plant height, number of pods, number of branches, seed yield, and oil percentage, and increased proline, phenolic, anthocyanin, and glycine betaine contents. The evaluated genotypes exhibited varied responses to drought-stress conditions. The developed tissue-cultured genotypes T2, T3, and T1, as well as exotic genotype Torpe, possessed the highest performance in all evaluated parameters and surpassed the other tested genotypes under water-deficit conditions. Overall, our findings elicited the superiority of certain newly developed tissue-cultured genotypes and exotic ones compared with commercial cultivars, which could be exploited in canola breeding under water-deficit conditions.
Effects of Trehalose Supplementation on Lipid Composition of Rooster Spermatozoa Membranes in a Freeze/Thaw Protocol
Authors: Stanishevskaya Olga I. , Silyukova Yulia , Fedorova Elena , Pleshanov Nikolai , Kurochkin Anton , Tereshina Vera M. , Ianutsevich Elena
Published: March 2023

The plasma membrane of spermatozoa plays an important role in the formation and maintenance of many functions of spermatozoa, including during cryopreservation. As a result of chromatographic analysis, the content of lipids and fatty acids in the membranes of spermatozoa of roosters of two breeds was determined under the influence of cryoprotective media containing trehalose LCM-control (0 mM), Treh20 (9.5 mM), and Treh30 (13.4 mM). The use of the cryoprotective diluent Treh20 made it possible to maintain a dynamic balance between the synthesis and degradation of phospholipids and sterols in the plasma membranes of frozen/thawed spermatozoa, close to that of native spermatozoa. This contributed to an increase in the preservation of frozen/thawed spermatozoa membranes from 48.3% to 52.2% in the egg breed and from 30.0% to 35.1% in the meat- and-egg breed. It was also noted that their kinetic apparatus (mobility indicators) remained at the level of 45.6% (egg breed) and 52.4% (meat-and-egg breed). An increase in the concentration of trehalose to 13.4 mM in a cryoprotective diluent for rooster sperm resulted in a decrease in the morphofunctional parameters of frozen/thawed spermatozoa.
Ante-mortem and Post-mortem Inspection and Relationship between Findings in a North Albanian Pig Slaughterhouse
Authors: Andoni Egon , Cocoli Sonila , Miraglia Dino , Balzaretti Claudia M. , Brecchia Gabriele , Bijo Bizena , Menchetti Laura , Musa Laura , Curone Giulio , Agradi Stella , Kumbe Ilirian , Zalla Pellumb , Gjoni Edison , Bixheku Xhilola , Castrica Marta
Published: March 2023

In June 2014, Albania was granted EU candidate status, thus starting a process of compliance with the membership criteria. In this context, a modern meat inspection approach in line with the European legislation was applied to a pig slaughterhouse in northern Albania in order to investigate the ante-mortem (AM) and post-mortem (PM) conditions and the relationship between these findings. For this purpose, 3930 pigs divided into 35 batches were evaluated over a 3-month period. The most frequent AM conditions recorded were tail lesions and dyspnea (9.1%), followed by skin (8.9%) and ear lesions (8.5%), while in the PM inspections, pleuritis was the most frequently observed condition (10.2%), followed by pneumonia (8.5%), liver alterations (5.7%), milk spot liver (3.8%), and pericarditis (3.3%). With the exception of liver alterations, the other PM lesions mentioned were positively associated with lesions on the ears (OR = 1.036; p < 0.001) and skin (OR = 1.026; p = 0.011) and dyspnea (OR = 1.021; p = 0.005), confirming the link between these variables and the health and welfare conditions of pigs on farms. Overall, the evidence that emerged from this Albanian slaughterhouse can be considered in line with other European contexts, especially in light of the considerable variability in the data present in the literature.
Metabolic Rates of Japanese Anchovy (Engraulis japonicus) during Early Development Using a Novel Modified Respirometry Method
Authors: Kim Dong In
Published: March 2023

The allometric relationship between metabolic rate (VO2) and body mass (M) has been a subject of fascination and controversy for decades. Nevertheless, little is known about intraspecific size-scaling metabolism in marine animals such as teleost fish. The Japanese anchovy Engraulis japonicus is a planktotrophic pelagic fish with a rapid growth and metabolic rate. However, metabolic rate measurements are difficult in this species due to their extremely small body size after hatching. Herein, the metabolic rate of this species during its early developmental stage was measured for 47 individuals weighing 0.00009–0.09 g (from just after hatching to 43 days old) using the micro-semi-closed method, a newly modified method for monitoring metabolism developed specifically for this study. As a result, three distinct allometric phases were identified. During these phases, two stepwise increases in scaling constants occurred at around 0.001 and 0.01 g, although the scaling exponent constant remained unchanged in each phase (b^ = 0.683). Behavioral and morphological changes accompanied the stepwise increases in scaling constants. Although this novel modified respirometry method requires further validation, it is expected that this study will be useful for future metabolic ecology research in fish to determine metabolism and survival strategy.
Diet and Plastic Ingestion in the Blackmouth Catshark Galeus melastomus, Rafinesque 1810, in Italian Waters
Authors: Zicarelli Giorgia , Romano Chiara , Gallo Samira , Valentino Carmen , Pepe Bellomo Victor , Leonetti Francesco Luigi , Giglio Gianni , Neri Alessandra , Marsili Letizia , Milazzo Concetta , Faggio Caterina , Mancusi Cecilia , Sperone Emilio
Published: March 2023

Galeus melastomus is the most common Pentanchidae in the Mediterranean Sea. A scavenger and opportunistic feeder, and despite the wide distribution, little is known about its feeding habits in Italian waters. The main purpose of this study was to investigate the diet of the blackmouth catshark by analysing the stomach contents. The specimens analysed were obtained from five populations of the Tyrrhenian and of the Ionian Seas, collected from a depth between 40 and 700 m. A total of 259 stomachs were analysed. The stomach contents were grouped into macro-categories and identified to the lowest taxonomic level possible. Crustaceans such as Parapenaeus longirostris, the Cephalopods Heteroteuthis dispar and Onychoteuthis banksii, and Osteichthyes, mostly Myctophidae, were identified. Plastic debris was also found among the stomach contents and classified according to its colour and shape. Osteichthyes represent the most abundant item (44%), above all the Myctophidae family, except for the catshark population from Tuscany, in which the most frequent species were Cephalopods, such as Abralia veranyi and Heteroteuthis dispar. Differences in the plastic debris contents were also observed between the Tuscany population and other populations. These could be explained as a probable consequence of the different depths at which the blackmouth catshark populations were sampled.
Safety, Tolerability, and Immunogenicity of V160, a Conditionally Replication-Defective Cytomegalovirus Vaccine, in Healthy Japanese Men in a Randomized, Controlled Phase 1 Study
Authors: Murata Shinya , Oshima Nobuyuki , Iwasa Takashi , Fukao Yukako , Sawata Miyuki
Published: March 2023

Cytomegalovirus (CMV) infection can cause newborn morbidity and mortality; no pharmacological method of reducing CMV infection during pregnancy is currently available. In a phase 1 study in the United States, V160, a conditionally replication-defective CMV vaccine, was immunogenic and well tolerated. This placebo-controlled study (NCT03840174) investigated the safety and immunogenicity of a three-dose V160 vaccine administered over six months. A total of 18 healthy adult Japanese males (9 seronegative and 9 seropositive) were enrolled at a single center and randomized 2:1 to intramuscular V160 or placebo. In vitro, V160 induced high CMV-specific neutralizing antibody (NAb) titers (50% neutralization titer [NT50], 3651; 95% confidence interval [CI], 1688–7895) in the CMV-seronegative per-protocol immunogenicity (PPI) population one month after the third vaccine dose was administered compared with no change in the placebo arm (NT50, <94; 95% CI <94–115). The geometric mean titer ratio in the seronegative population versus baseline was 77.7 (95% CI, 23.9–252.4). CMV NAb titers in the CMV-seropositive PPI population were similar to baseline NAb titers observed in the CMV-seropositive population. V160 was well tolerated, and no vaccine viral DNA shedding was observed. In conclusion, the immunogenicity and safety profile of V160 in Japanese participants was consistent with other populations.
Green Synthesis of Silver Nanoparticles Using the Leaf Extract of the Medicinal Plant, Uvaria narum and Its Antibacterial, Antiangiogenic, Anticancer and Catalytic Properties
Authors: Ajaykumar Anthyalam Parambil , Mathew Anjaly , Chandni Ayanam Parambath , Varma Sudhir Rama , Jayaraj Kodangattil Narayanan , Sabira Ovungal , Rasheed Vazhanthodi Abdul , Binitha Valiyaparambil Sivadasan , Swaminathan Thangaraj Raja , Basheer Valaparambil Saidumohammad , Giri Suvendu , Chatterjee Suvro
Published: March 2023

Silver nanoparticles (AgNPs) made by green synthesis offer a variety of biochemical properties and are an excellent alternative to traditional medications due to their low cost. In the current study, we synthesised AgNPs from the leaf extract of the medicinal plant Uvaria narum, commonly called narumpanal. The nanoparticles were characterised by ultraviolet-visible (UV-Vis) spectroscopy, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). SEM analysis showed AgNPs are highly crystalline and spherical with an average diameter of 7.13 nm. The outstanding catalytic activity of AgNPs was demonstrated by employing the reduction of 4-nitrophenol to 4-aminophenol. The AgNPs showed antiangiogenic activity in the chick chorioallantoic membrane (CAM) assay. AgNPs demonstrated anticancer activity against Dalton’s lymphoma ascites cells (DLA cells) in trypan blue assay and cytotoxicity against three fish cell lines: Oreochromis niloticus liver (onlL; National Repository of Fish Cell Lines, India (NRFC) Accession number—NRFC052) cells, Cyprinus carpio koi fin (CCKF; NRFC Accession number—NRFC007) cells and Cyprinus carpio gill (CyCKG; NRFC Accession number—NRFC064). Furthermore, the AgNPs demonstrated their ability to inhibit pathogenic microorganisms, Staphylococcus aureus, and Escherichia coli. The results from the study displayed green synthesised AgNPs exhibit antiangiogenic activity, cytotoxicity, antimicrobial and catalytic properties, which are crucial characteristics of a molecule with excellent clinical applications.
Antimicrobial and Antibiofilm Potential of Thymus vulgaris and Cymbopogon flexuosus Essential Oils against Pure and Mixed Cultures of Foodborne Bacteria
Authors: Monteiro Marques Joana , Serrano Susana , Selmi Hiba , Giesteira Cotovio Pedro , Semedo-Lemsaddek Teresa
Published: March 2023

The spread of pathogenic and food spoilage microorganisms through the food chain still faces major mitigation challenges, despite modern advances. Although multiple cleaning and disinfection procedures are available for microbial load reduction in food-related settings, microbes can still remain on surfaces, equipment, or machinery, especially if they have the ability to form biofilms. The present study assessed the biofilm-forming properties of pure and mixed cultures of foodborne and spoilage bacteria (Listeria monocytogenes, Enterococcus faecalis, Aeromonas hydrophila, Brochothrix thermosphacta), using polystyrene and stainless steel contact surfaces. Subsequently, the antimicrobial and antibiofilm properties of Thymus vulgaris and Cymbopogon flexuosus essential oils—EOs—were evaluated against these bacteria. Moreover, in silico prediction of the absorption and toxicity values of the EOs’ major constituents was also performed, perceiving the putative application in food-related settings. Overall, biofilm formation was observed for all microbes under study, at different temperatures and both contact surfaces. In polystyrene, at 25 °C, when comparing pure with mixed cultures, the combination Listeria–Aeromonas achieved the highest biofilm biomass. Moreover, at 4 °C, increased biofilm formation was detected in stainless steel. Regarding thyme, this EO showed promising antimicrobial features (especially against A. hydrophila, with a MIC of 0.60 µg/µL) and antibiofilm abilities (MBEC of 110.79 µg/µL against L. monocytogenes, a major concern in food settings). As for lemongrass EO, the highest antimicrobial activity, with a MIC of 0.49 µg/µL, was also observed against L. monocytogenes. Overall, despite promising results, the in situ effectiveness of these essential oils, alone or in combination with other antimicrobial compounds, should be further explored.
Coffee Consumption and the Risk of Metabolic Syndrome in the ‘Seguimiento Universidad de Navarra’ Project
Authors: Corbi-Cobo-Losey María J. , Martinez-Gonzalez Miguel Á. , Gribble Anne K. , Fernandez-Montero Alejandro , Navarro Adela M. , Domínguez Ligia J. , Bes-Rastrollo Maira , Toledo Estefanía
Published: March 2023

(1) Background: Metabolic Syndrome (MetS) affects over a third of the United States population, and has similar prevalence in Europe. Dietary approaches to prevention are important. Coffee consumption has been inversely associated with mortality and chronic disease; however, its relation to the risk of MetS is unclear. We aimed to investigate the association between coffee consumption and incident MetS in the ‘Seguimiento Universidad de Navarra’ cohort. (2) Methods: From the SUN project, we included 10,253 participants initially free of MetS. Coffee consumption was assessed at baseline, and the development of MetS was assessed after 6 years of follow-up. All data were self-reported by participants. MetS was defined according to the Harmonizing Definition. We used multivariable logistic regression models to estimate odds ratios and 95% confidence intervals for incident MetS according to four categories of coffee consumption: <1 cup/month; ≥1 cup/month to <1 cup/day; ≥1 cup/day to <4 cups/day; ≥4 cups/day. (3) Results: 398 participants developed MetS. Coffee consumption of ≥1 to <4 cups/day was associated with significantly lower odds of developing MetS (multivariable adjusted OR = 0.71, 95% CI (0.50–0.99)) as compared to consumption of <1 cup/month. (4) Conclusions: In a Mediterranean cohort, moderate coffee consumption may be associated with a lower risk of MetS.
The Impact of Plasma Membrane Ion Channels on Bone Remodeling in Response to Mechanical Stress, Oxidative Imbalance, and Acidosis
Authors: Perin Martina , Chinigò Giorgia , Genova Tullio , Mussano Federico , Munaron Luca
Published: March 2023

The extracellular milieu is a rich source of different stimuli and stressors. Some of them depend on the chemical–physical features of the matrix, while others may come from the ‘outer’ environment, as in the case of mechanical loading applied on the bones. In addition to these forces, a plethora of chemical signals drives cell physiology and fate, possibly leading to dysfunctions when the homeostasis is disrupted. This variety of stimuli triggers different responses among the tissues: bones represent a particular milieu in which a fragile balance between mechanical and metabolic demands should be tuned and maintained by the concerted activity of cell biomolecules located at the interface between external and internal environments. Plasma membrane ion channels can be viewed as multifunctional protein machines that act as rapid and selective dual-nature hubs, sensors, and transducers. Here we focus on some multisensory ion channels (belonging to Piezo, TRP, ASIC/EnaC, P2XR, Connexin, and Pannexin families) actually or potentially playing a significant role in bone adaptation to three main stressors, mechanical forces, oxidative stress, and acidosis, through their effects on bone cells including mesenchymal stem cells, osteoblasts, osteoclasts, and osteocytes. Ion channel-mediated bone remodeling occurs in physiological processes, aging, and human diseases such as osteoporosis, cancer, and traumatic events.
Antibacterial Activity of ZnSe, ZnSe-TiO2 and TiO2 Particles Tailored by Lysozyme Loading and Visible Light Irradiation
Authors: Anastasescu Crina , Neagu Simona , Preda Silviu , Culita Daniela , Stancu Mihaela , Banciu Cristian , Munteanu Cornel , Bratan Veronica , Calderon-Moreno Jose Maria , State Razvan , Anastasescu Mihai , Enache Madalin , Balint Ioan , Zaharescu Maria
Published: March 2023

ZnSe, ZnSe-TiO2 microspheres and nanostructured TiO2 obtained by hydrothermal and sol–gel methods were tested against Staphylococcus aureus ATCC 25923 and Micrococcus lysodeikticus ATCC 4698 before and after lysozyme (Lys) loading. Morphological characterization of inorganic matrices and hybrid organic–inorganic complexes were performed by microscopy techniques (SEM, AFM and Dark Field Hyperspectral Microscopy). Light absorption properties of ZnSe, ZnSe-TiO2 and TiO2 powders were assessed by UV–visible spectroscopy and their ability to generate reactive oxygen species (•OH and O2•−) under visible light irradiation was investigated. Antibacterial activity of ZnSe, ZnSe-TiO2, TiO2, Lys/ZnSe, Lys/ZnSe-TiO2 and Lys/TiO2 samples under exposure to visible light irradiation (λ > 420 nm) was tested against Staphylococcus aureus and Micrococcus lysodeikticus and correlated with ROS photogeneration.
Dimethyl Itaconate Inhibits Melanogenesis in B16F10 Cells
Authors: Yu Bo-Yeong , Ngo Hoang , Choi Won , Keum Young-Sam
Published: March 2023

Itaconate is a metabolite produced to counteract and resolve pro-inflammatory responses when macrophages are challenged with intracellular or extracellular stimuli. In the present study, we have observed that dimethyl itaconate (DMI) inhibits melanogenesis in B16F10 cells. DMI inhibits microphthalmia-associated transcription factor (MITF) and downregulates the expression of MITF target genes, such as tyrosinase (TYR), tyrosinase-related protein 1 (TRP-1), and tyrosinase-related protein 2 (TRP-2). DMI also decreases the level of melanocortin 1 receptor (MC1R) and the production of α-melanocyte stimulating hormone (α-MSH), resulting in the inhibition of extracellular signal-regulated kinase 1/2 (ERK1/2) and MITF activities. The structure–activity relationship (SAR) study illustrates that the α,β-unsaturated carbonyl moiety in DMI, a moiety required to target KELCH-like ECH-associated protein 1 (KEAP1) to activate NF-E2-related factor 2 (NRF2), is necessary to inhibit melanogenesis and knocking down Nrf2 attenuates the inhibition of melanogenesis by DMI. Together, our study reveals that the MC1R-ERK1/2-MITF axis regulated by the KEAP1-NRF2 pathway is the molecular target responsible for the inhibition of melanogenesis by DMI.
Cytoprotective Effect of Pteryxin on Insulinoma MIN6 Cells Due to Antioxidant Enzymes Expression via Nrf2/ARE Activation
Authors: Taira Junsei , Tsuda Ryuji , Miyagi-Shiohira Chika , Noguchi Hirofumi , Ogi Takayuki
Published: March 2023

The low-level antioxidant activity of pancreatic islets causes type 1 diabetes due to oxidative stress, which is also the cause of failure in the pancreatic islets’ isolation and cell transplantation. In our previous study, pteryxin was found to be a natural product as a nuclear factor-erythroid-2-related factor (Nrf2) activator. This study focused on elucidation that the potentiality of pteryxin can activate the antioxidant enzymes, even under oxidative stress, by hydrogen peroxide (H2O2). Pteryxin treated with mouse insulinoma MIN6 cells was enhanced the antioxidant gene expressions in the ARE (antioxidant response element) region for HO-1 (Heme Oxygenase-1), GCLC (Glutamate-cysteine ligase catalytic subunit), SOD1 (Super Oxide dismutase1), and Trxr1 (Thioredoxin reductase1), and those enzymes were also expressed during the nuclei transference of cytoplasmic Nrf2. In fact, the cells exposed to H2O2 concentrations of a half-cell lethal in the presence of pteryxin were then induced main antioxidant enzymes, HO-1, GCLC, and Trxr1 in the ARE region. The increased glutathione (GSH) levels associated with the GCLC expression also suggested to be cytoprotective against oxidative stress by activating the redox-metabolizing enzymes involving their increased antioxidant activity in the cells. In addition, Akt is a modulator for Nrf2, which may be responsible for the Nrf2 activation. These results allowed us to consider whether pteryxin or its synthesized congeners, an Nrf2 activator, is a potential preservative agent against islet isolation during cell transplantation.
Aging-Related Ovarian Failure and Infertility: Melatonin to the Rescue
Authors: Reiter Russel J. , Sharma Ramaswamy , Romero Alejandro , Manucha Walter , Tan Dun-Xian , Zuccari Debora Aparecida Pires de Campos , Chuffa Luiz Gustavo de Almeida
Published: March 2023

Aging has a major detrimental effect on the optimal function of the ovary with changes in this organ preceding the age-related deterioration in other tissues, with the middle-aged shutdown leading to infertility. Reduced fertility and consequent inability to conceive by women in present-day societies who choose to have children later in life leads to increased frustration. Melatonin is known to have anti-aging properties related to its antioxidant and anti-inflammatory actions. Its higher follicular fluid levels relative to blood concentrations and its likely synthesis in the oocyte, granulosa, and luteal cells suggest that it is optimally positioned to interfere with age-associated deterioration of the ovary. Additionally, the end of the female reproductive span coincides with a significant reduction in endogenous melatonin levels. Thus, the aims are to review the literature indicating melatonin production in mitochondria of oocytes, granulosa cells, and luteal cells, identify the multiple processes underlying changes in the ovary, especially late in the cessation of the reproductive life span, summarize the physiological and molecular actions of melatonin in the maintenance of normal ovaries and in the aging ovaries, and integrate the acquired information into an explanation for considering melatonin in the treatment of age-related infertility. Use of supplemental melatonin may help preserve fertility later in life and alleviate frustration in women delaying childbearing age, reduce the necessity of in vitro fertilization–embryo transfer (IVF-ET) procedures, and help solve the progressively increasing problem of non-aging-related infertility in women throughout their reproductive life span. While additional research is needed to fully understand the effects of melatonin supplementation on potentially enhancing fertility, studies published to date suggest it may be a promising option for those struggling with infertility.