bluuebunny/crossref_metadata_2025 huggingface dataset:
Effect of Forward and Reverse Suturing on Nerve Regeneration Following Facial Nerve Axotomy
Authors: Lee Jae Min , Chung Jong Woo , Jeong Na Young , Jung Junyang , Kim Sung Soo , Jeong Su Jin , Yeo Seung Geun
Published: November 2024

Background/Objectives: When the facial nerve is severed and a nerve graft is required, motor nerves are typically connected in the forward direction, while sensory nerves are connected in the reverse direction. However, there is limited research on the effects of reversing this connection, and no studies have been conducted using the same facial nerve. This study aimed to investigate the effects of forward and reverse suturing on nerve regeneration following facial nerve axotomy. Methods: The facial nerve trunk of male Sprague Dawley rats was incised to induce facial nerve injury, and autografts were sutured using both forward and reverse methods. Behavioral tests, including whisker reflex and eye blink tests, were conducted. Histological analyses, including toluidine blue staining and transmission electron microscopy (TEM), were performed to evaluate axon recovery. Results: Behavioral experiments showed signs of recovery at 3–4 weeks in both the forward and reverse suture groups, with no significant differences between the two methods (p < 0.01). Histological analysis showed partial recovery by 8 weeks in both groups. Toluidine blue staining indicated a reduction in the number of axons at 4 weeks, with partial recovery at 8 weeks (p < 0.001) in both groups. TEM analysis revealed that myelin fiber thickness was restored in both the forward and reverse suture groups, though it remained thinner compared to normal (p < 0.01). Conclusions: Our results suggest that the direction of nerve suturing (forward vs. reverse) does not significantly impact nerve regeneration or functional recovery. Both suturing methods demonstrated similar recovery effects, with no significant differences in microstructural regeneration. Future studies should investigate the molecular mechanisms underlying nerve regeneration and extend the observation period to provide a more comprehensive understanding of this process.
External Scaffold for Strengthening the Pulmonary Autograft in the Ross Procedure
Authors: Nappi Francesco , Nassif Aubin , Schoell Thibaut
Published: November 2024

Despite offering several potential benefits over standard prosthetic aortic valve replacement, the use of the pulmonary autograft has been limited to date due to concerns over the risk of pulmonary autograft expansion and the need for reintervention. Several techniques using materials with biomimetic potential have been developed to reduce this complication. The incidence, risk factors, and pathophysiology of pulmonary autograft dilatation are discussed in this article. This seminar will provide an overview of the techniques of external pulmonary autograft support and their advantages and limitations. It also considers future directions for further investigation and future clinical applications of external pulmonary autograft support. Dilatation of the autograft is more likely to occur in patients with aortic regurgitation and a dilated aortic annulus. External scaffolding may prevent autograft stretching and expansion in these specific cases. However, from a biomimetic point of view, any permanent scaffold potentially restricts the movement of the autograft root. This reduces some of the benefits associated with the use of autologous tissue, which is the priority of the Ross procedure. To address this issue, several bioresorbable matrices could be used to support the root during its initial adaptive phase. Control of blood pressure with aggressive therapy is the first line to avoid this problem in the first year after pulmonary autograft implantation, together with support of the annular and sinotubular junction in some selected cases. This is the best way to maintain stable autograft root dimensions while preserving root dynamics. However, to determine the efficacy of this combined external support and best medical management, it is important to perform regular imaging and clinical follow-up.
Anthropomorphic Design in Mortality Salience Situations: Exploring Emotional and Non-Emotional Mechanisms Enhancing Consumer Purchase Intentions
Authors: Sun Cong , Ding Yuechun , Wang Xinyi , Meng Xing
Published: November 2024

This study investigates the role of anthropomorphic design in alleviating consumer anxiety induced by mortality salience and delves into the underlying emotional and non-emotional mechanisms. Through a series of meticulously designed experiments, we confirm that anthropomorphic design significantly enhances positive emotional responses in consumers, reduces negative emotions, and thereby increases their preference and willingness to purchase products. Even after the diminution of emotional reactions, anthropomorphic design continues to sustain consumer preference by enhancing psychological intimacy. These findings reveal the crucial role of anthropomorphic design as an effective emotional regulation strategy in consumer purchasing behavior, enriching the application of terror management theory and emotion regulation theory in consumer behavior research. Furthermore, our study provides valuable practical guidance for product design and marketing strategies, especially for consumer groups frequently facing high-pressure situations. Products with anthropomorphic designs may be more appealing to these consumers, helping to mitigate their death anxiety and enhance psychological well-being.
Psychophysiological and Performance Effects of Biofeedback and Neurofeedback Interventions in a Top 100 Female Chess Player
Authors: Fuentes-García Juan Pedro , Villafaina Santos
Published: November 2024

(1) Background: Previous studies showed that neurofeedback and biofeedback could improve stress levels, enhance self-control over physiological factors, improve behavioral efficiency, and increase reaction speed to stimuli. Specifically, the sensorimotor rhythm stimulation (12–15 Hz) can enhance cognitive functions such as selective attention and working memory. However, there is no study that analyzes the effect of these interventions in chess players. (2) Methods: A Chess Woman Grandmaster and Chess International Master, with an ELO ranking higher than 2350 points, was selected to participate in this case study. The participant conducted a total of 14 sessions of biofeedback and neurofeedback, training in breathing, sensorimotor rhythm stimulation in Cz, skin conductance, temperature, and heart rate variability combined with chess work. Specific and non-specific tasks were designed to evaluate the intervention. (3) Results: The chess player enhanced the heart rate variability during specific and non-specific chess tasks: chess problems, 15 + 10 games, and puzzle rush games. In addition, the sensorimotor rhythm power decreased during the chess problem and increased during the 15 + 10 game and puzzle rush. Also, chess performance and anxiety levels improved after the intervention. (4) Conclusions: Neurofeedback and biofeedback training combined with chess training could improve the performance of chess players.
The Effect of Corporate Social Responsibility Characteristics on Employee Green Behavior: A Moral Emotions Perspective
Authors: Zhang Na , Ren Xiaoyu , Sun Xiang , Jin Chunhua
Published: November 2024

Shaping green employees is a source of sustainable competitive advantage for enterprises. However, previous studies have lacked consideration of the moral aspects of employee green behavior (EGB), thus ignoring the mechanism of employee moral psychology, especially the important impact of moral emotions on EGB. Based on the affective events theory, we built a moderated mediation model to test how corporate social responsibility characteristics (CSRCs) affect EGB and to explore the role of organizational pride (OP) and the environmental passion climate (EPC) in this process. SPSS25.0 and Mplus8.0 were used to analyze data from 267 valid questionnaires. The results show that CSRCs’ positive effect required EGB and OP to play a mediating role in the relationship between them. In addition, EPC had a negative moderating effect on the effect of OP on required EGB. These results expand the theoretical territory of EGB from the perspective of moral emotions. In practice, the results provide a moral motivation for cultivating employees’ ecological literacy and stimulating EGB as well as management strategies for organizational environmental protection practice and green sustainable development.
Thriving Beyond Adversity: A Prospective Longitudinal Cohort Study Using a Strength-Based Approach Depicts Indigenous Adolescents with Less Adverse Childhood Experiences (ACEs) Had Fewer Neurodevelopmental Disorders (NDDs)
Authors: Islam Md Irteja , Lam Bernadette Yan Yue , Esgin Tuguy , Martiniuk Alexandra
Published: November 2024

Improving social and emotional well-being (SEWB) among Indigenous adolescents is crucial. Since neurodevelopmental disorders (NDDs) are common in Indigenous people and adverse childhood experiences (ACEs) are important contributors to negative health outcomes throughout the lifespan, we investigated whether limited ACE exposure is associated with reduced risk of NDDs in Australian Indigenous teens using the data from multiple waves (Wave 1 to Wave 9, and Wave 11) of the Longitudinal Study of Indigenous Children (LSIC). We also examined the role of other protective factors, such as Indigenous cultural identity and school connectedness, against NDDs. A strengths-based approach using mixed-effects logistic regression models examined the protective effect of limited ACE exposure (from LSIC waves 1–9) on NDDs (outcome from LSIC wave 11), adjusting for sociodemographic factors. The NDDs included autism, ADHD, intellectual, neurological, and specific learning disabilities. Of the 370 individuals analysed, 73.2% valued Indigenous cultural identity, and 70.5% were strongly connected at school. More than one-fourth (27.8%) reported limited ACE exposure, while the majority was not diagnosed with NDDs (93%). Longitudinal analysis revealed limited ACE exposure was 6.01 times (95% CI: 1.26–28.61; p = 0.024) more likely to be protective against NDDs compared to those exposed to multiple ACEs. Moreover, valuing cultural identity (aOR = 2.81; 95% CI: 1.06–7.39; p = 0.038) and girls (aOR = 13.88; 95% CI: 3.06–62.84; p = 0.001) were protective against NDDs compared to their respective counterparts. Our findings highlight the need to prevent ACE exposure and promote Indigenous cultural identity in preventing negative health outcomes and the exacerbation of health inequities to strengthen the SEWB of Indigenous communities.
Fracture Mode and Thermal Damage Evolution of Sandstone Under the Coupling Effect of Thermal Treatment and Impact Load
Authors: Xi Yan , Wang Yanglin , Yin Jianwei , Jiang Hailong , Wang Wei
Published: November 2024

The dynamic properties of high-temperature sandstone quickly deteriorate with different cooling methods, which leads to the instability of underground engineering rock structures. Therefore, it is of great significance to quantify the changes in the dynamic characteristics of high-temperature cooled sandstone under impact loads. Therefore, the sandstone is heated to different temperatures and cooled using three methods. A dynamic tensile test is performed using the Splitting Hopkinson Pressure Bar (SHPB) test set for high-temperature cooled sandstone. At the same time, the transient process of rock failure was examined using high-speed cameras. The influence of different temperatures and cooling methods on the thermal damage value of sandstone was analyzed, and the prediction equation was formed. The change in rock energy during rock failure under impact load was calculated.
A Study on the Visual Perception of Cultural Value Characteristics of Traditional Southern Fujian Architecture Based on Eye Tracking
Authors: Deng Jianxun , Chen Junlong , Lei Ying
Published: November 2024

The traditional Southern Fujian architecture serves as a reflection of the region’s deeply rooted cultural values. However, research on the architectural and cultural heritage of Southern Fujian remains scarce. Accordingly, this study employs eye-tracking technology to experiment with 20 individuals with local cultural backgrounds and 20 without it to investigate the differences in the perception of the architectural heritage of Southern Fujian by individuals with disparate cultural backgrounds. The findings indicate that distinct groups evince disparate visual perceptions of the architectural and cultural heritage of Southern Fujian. The participants’ attention was divided into two categories: patterns and shapes and calligraphy and text. Non-locals were primarily drawn to patterns and shapes, whereas locals demonstrated a greater interest in the calligraphy and text category. The extraction of cultural information from calligraphic characters requires greater understanding and more time spent within the culture. Knowledge of local cultural backgrounds is helpful to better understand such information. This highlights the potential impact of Southern Fujian architecture on the local population. These findings provide a compelling rationale for the incorporation of multicultural elements in contemporary architectural design while also advocating for the preservation and advancement of Southern Fujian architectural heritage.
Research on Digital Construction Technology for Special-Shaped Shell Pipe Structures
Authors: Zhao Wenyan , Zha Xiaoxiong , Pan Wenzhi , Chen Zhaohong
Published: November 2024

The aesthetic appeal of special-shaped shell pipe structures makes them highly favored by architects and holds promising prospects for various applications. In the detailed design stage, NURBS curves should be divided into multiple continuous arcs due to the limitations of current steel structure fabrication equipment, which can only accommodate pipes with equal-curvature bends. However, the traditional manual fitting methods suffer from several issues including low efficiency, undercutting at the interface, poor smoothness of curves, and lack of control over tolerances. Furthermore, the weaker out-of-plane stiffness and utilization of bending arc pipe sections pose significant challenges in terms of spatial positioning and installation accuracy that need to be addressed. The study focuses on addressing these challenges by investigating digital construction technology for special-shaped shell pipe structures and developing a parametric algorithm that enables automatic fitting of spatial NURBS curves into multiple arcs, thereby achieving seamless curve fitting. A post-processing program was developed to enable the parametric generation of fabrication and installation information for structural members, which can be seamlessly integrated into the BIM database. Finally, structural position control technology is proposed to improve assembly efficiency and ensure consistency between the completed construction state and the design shape. The above digital construction technology has been applied in projects such as the Haihua Island International Conference Center. It can provide complete technical solutions for modeling of special-shaped shell pipe structures, including establishment of a member information database, fabrication at the workshop and installation on site, construction organization management, as well as installation accuracy control.
Identification Model of Traditional Village Cultural Landscape Elements and Its Application from the Perspective of Living Heritage—A Case Study of Chentian Village in Wuhan
Authors: Li Rui , Zhang Yawei , Li Wanshi , Xu Xuanxuan
Published: November 2024

At present, there are limitations in the cognition of the elements of traditional village cultural landscapes, less excavation of the elements of production-type cultural landscapes, little attention to the layering of cultural landscapes in different periods and the correlation between the elements of different types of cultural landscapes, and insufficient research on the living continuity of traditional village cultural landscapes. In the context of rural cultural revitalization, it is necessary to explore strategies for the conservation and development of traditional village cultural landscapes from the perspective of living heritage. By interpreting the core connotation of the concepts of living heritage and cultural landscape, a model of traditional village cultural landscape elements and a framework for the identification of living elements are constructed. On the above basis, Chentian Village in Wuhan is taken as an example to identify its living cultural landscape elements, and to analyze the evolution of its cultural landscape elements from the dimension of “time-function”. In Chentian Village, a total of seventeen cultural landscapes were identified. Of these, ten were cultural landscapes with functional continuity and living heritage characteristics; three were cultural landscapes whose existing functions have changed significantly from their initial functions; and four were cultural landscapes that were inactive and have lost their original functions. Finally, this study proposes corresponding protection and development strategies for living heritage and non-living heritage of cultural landscapes.
The Influence of Shear Connectors in a Longitudinal Arrangement on Free Vibrations of Steel–Concrete Composite Beams
Authors: Sun Qikai , Zhang Chao , Yan Yateng , Cai Chaoxun , Li Kebing , Zhang Nan
Published: November 2024

In this work, the role of longitudinal shear connectors in mitigating free vibrations of steel–concrete composite beams (SCCBs) is scrutinized utilizing an innovative finite element methodology. In the proposed methodology, partial shear connection between the adjacent sub-beams is considered, and the SCCBs with various stiffnesses are modeled under four common boundary conditions, namely, (a) simply supported ends (S-S), (b) one clamped end and one free end (C-F), (c) one clamped end and one simply supported end (C-S), and (d) clamped ends (C-C). Thereafter, successful verification of the proposed methodology’s efficacy is achieved through comparative analysis with the theoretical results. Lastly, a reasonable arrangement along this longitudinal direction of shear connectors is proposed based on the change law of the squared fundamental frequency ratio.
Design and Seismic Performance Study of Multistage Controllable Isolation Bearing for High-Speed Railway Simply Supported Beam
Authors: Liu Hanyun , Jiang Jun , Mao Na , Mao Yingyu , Mao Jianfeng
Published: November 2024

The high-speed railway (HSR) system imposes stringent requirements for track smoothness. However, conventional seismic isolation bearings frequently fail to meet these demands. To address this challenge, a novel seismic isolation bearing was developed based on the principle of functional separation design. This innovative bearing effectively achieves the multistage control objectives, including amplitude limitation to ensure track smoothness during frequent earthquakes, energy dissipation to guarantee train running safety during design earthquakes, and structural integrity maintenance to prevent beam collapse during rare earthquakes. Firstly, an overview of the novel isolation bearing’s structural design and operational principle is provided. Subsequently, a corresponding mechanical model is formulated, with the parameters of the new bearing determined through finite element analysis. The study then compares the seismic performance of the general rubber bearing and the new bearing, using an HSR simply supported bridge as an engineering background. The dynamic response of the bridge under varying seismic waves, pier heights, and bridge spans is meticulously analyzed. The results indicate that the new bearing can achieve multistage control. Compared to general bearings, it reduces bridge displacement vibration by over 46.4% under frequent, design, and rare earthquakes. The bridge deformation under frequent earthquakes remains below 3 mm, thus meeting the track smoothness requirements for normal HSR operations. Additionally, the study reveals that higher pier heights increase the seismic response, peaking at 15 m. The vibration reduction provided by the new bearing varies but remains effective in most earthquake scenarios, with maximum reductions of 92.9% for displacement and 74.17% for bending moment. Furthermore, larger bridge spans also increase the seismic response, with the 24 m span bridge outperforming the 32 m span bridge. In conclusion, the novel seismic isolation bearing significantly enhances the seismic performance of HSR bridges, ensuring train running safety and operational reliability.
Targeting the EGFR and Spindle Assembly Checkpoint Pathways in Oral Cancer: A Plausible Alliance to Enhance Cell Death
Authors: Calheiros-Lobo Mafalda , Silva João P. N. , Delgado Leonor , Pinto Bárbara , Monteiro Luís , Lopes Carlos , Silva Patrícia M. A. , Bousbaa Hassan
Published: November 2024

Background/Objectives: Head and neck cancer (HNC) is among the most common cancer types globally, with its incidence expected to increase significantly in the coming years. Oral squamous cell carcinoma (OSCC), the predominant subtype, exhibits significant heterogeneity and resistance to treatment. Current therapies, including surgery, radiation, and chemotherapy, often result in poor outcomes for advanced stages. Cetuximab, an EGFR inhibitor, is widely used but faces limitations. This study explores the combined inhibition of EGFR and mitotic proteins to enhance treatment efficacy. Methods: We analyzed the effects of co-treating OSCC cells with small molecules targeting MPS-1 (BAY1217389), Aurora-B (Barasertib), or KSP (Ispinesib), alongside Cetuximab. The rationale is based on targeting EGFR-mediated survival pathways and the mitotic checkpoint, addressing multiple cell cycle phases and reducing resistance. Results: Our findings indicate that inhibiting MPS-1, Aurora-B, or KSP enhances Cetuximab’s therapeutic potential, promoting increased cancer cell death. Additionally, we examined EGFR, MPS-1, Aurora-B, and KSP expression in OSCC patient samples, revealing their clinicopathologic significance. Conclusions: This combinatorial approach suggests a promising strategy to improve treatment outcomes in OSCC.
An Integrated Multimodal-Based CAD System for Breast Cancer Diagnosis
Authors: Sunba Amal , AlShammari Maha , Almuhanna Afnan , Alkhnbashi Omer S.
Published: November 2024

Breast cancer has been one of the main causes of death among women recently, and it has been the focus of attention of many specialists and researchers in the health field. Because of its seriousness and spread speed, breast cancer-resisting methods, early diagnosis, diagnosis, and treatment have been the points of research discussion. Many computers-aided diagnosis (CAD) systems have been proposed to reduce the load on physicians and increase the accuracy of breast tumor diagnosis. To the best of our knowledge, combining patient information, including medical history, breast density, age, and other factors, with mammogram features from both breasts in craniocaudal (CC) and mediolateral oblique (MLO) views has not been previously investigated for breast tumor classification. In this paper, we investigated the effectiveness of using those inputs by comparing two combination approaches. The soft voting approach, produced from statistical information-based models (decision tree, random forest, K-nearest neighbor, Gaussian naive Bayes, gradient boosting, and MLP) and an image-based model (CNN), achieved 90% accuracy. Additionally, concatenating statistical and image-based features in a deep learning model achieved 93% accuracy. We found that it produced promising results that would enhance the CAD systems. As a result, this study finds that using both sides of mammograms outperformed the result of using only the infected side. In addition, integrating the mammogram features with statistical information enhanced the accuracy of the tumor classification. Our findings, based on a novel dataset, incorporate both patient information and four-view mammogram images, covering multiple classes: normal, benign, and malignant.
Enhanced Photodegradation of Sulfamethoxazole Through Cutting-Edge Titania-Zirconia-Based Materials
Authors: Bensmaine Zineb , El Korso Sanaa , Moral-Rodríguez Adriana Isabel , Bedrane Sumeya , Ziani-Cherif Chewki , Pérez-Cadenas Agustín Francisco , Carrasco-Marín Francisco , Bailón-García Esther
Published: November 2024

ZrO2, TiO2, ZrO2-TiO2, and TiO2-ZrO2 were successfully prepared using the sol–gel method and fully characterized to check their physico-chemical features. X-ray diffraction showed the co-existence of monoclinic and tetragonal ZrO2 in addition to the Anatase phase for TiO2. The formation of mixed oxides led to a reduction in the band gap values and a modification of the textural characteristics, while the XPS evidenced an oxygen vacancy-rich surface. The ability of the synthesized materials to eliminate drug contaminants was checked using Sulfamethoxazole (SMX) as a model molecule under UV and BLUE-LED irradiation. The materials’ potential to decrease wastewater toxicity was also studied. The best photocatalyst was TiO2-ZrO2 with 76 and 100% conversion under visible and UV irradiation, respectively.
Influence of Dopants on Pt/Al2O3-Based Monolithic Catalysts for Autothermal Oxidative Coupling of Methane
Authors: Schardt Sven , Bastian Simon , Çelik Ahmet , Chawla Jaspreet , Lott Patrick
Published: November 2024

Autothermal oxidative coupling of methane (OCM) is a highly attractive approach for methane utilization. If platinum-based catalysts are operated in short-contact-time reactors with high space velocities, high methane conversion can be achieved. Using a 1 wt.% Pt/Al2O3 catalyst as a benchmark, the present study elucidates how different dopants, namely Ni, Sn, and V2O5, affect the OCM reaction. Kinetic catalyst tests reveal that acetylene (C2H2) is the predominant C2 product, irrespective of the catalyst formulation or operation conditions. Furthermore, the use of bimetallic catalysts allows either for the maintenance or even the improvement of the C2 selectivity during OCM, which is attributed to synergistic effects that occur when expensive Pt is partially replaced by cheaper dopants. In particular, the 1 wt.% Pt/Al2O3 reference catalyst yielded a maximum C2 selectivity of 8.2%, whereas the best-performing doped sample 0.25 wt.% Pt 0.75 wt.% V2O5/Al2O3 yielded a total C2 selectivity of 11.3%.
Modification of High-Surface-Area Carbons Using Self-Limited Atomic Layer Deposition
Authors: Fan Mengjie , Shen Kai , Gorte Raymond J. , Vohs John M.
Published: November 2024

This study explores the application of Atomic Layer Deposition (ALD) to functionalize high-surface-area carbon supports with metal and metal oxide films and particles for applications in catalysis and electrocatalysis. The work reported here demonstrates that, through careful choice of precursors and absorption and reaction conditions, self-limited ALD growth on a high-surface-area carbon support can be achieved. Specific examples presented include the growth of conformal films of ZrO2 and SnO2 and the deposition of Ga2O3 and Pt particles on a carbon black support with a surface area of 250 m2·g−1. A novel strategy for controlling the Pt weight loading and producing sub-nanometer Pt particles on a carbon support using a single ALD cycle is also presented.
Advancements in Serine Protease Inhibitors: From Mechanistic Insights to Clinical Applications
Authors: Wei Yang , Huang Mingdong , Jiang Longguang
Published: November 2024

Serine proteases, a significant class of enzymes comprising approximately one-third of known human proteases, are ubiquitously present across various organisms. These enzymes typically exhibit highly conserved catalytic domain structures, and their activity is stringently regulated within the body, playing a pivotal role in numerous physiological processes. Dysregulation of serine protease activity can result in severe consequences, including excessive inflammation, heightened risk of thrombosis and cancer, and even mortality. Serine protease inhibitors have emerged as critical regulators, offering a broad range of physiological functions such as maintaining the coagulation–fibrinolysis balance, modulating inflammatory responses, accelerating wound healing, promoting apoptosis, and providing antitumor and antiviral effects. As a result, the development of serine protease inhibitors has become increasingly vital. In recent years, significant progress in the study of serine proteases has led to the pivotal role of various serine protease inhibitors in clinical diagnosis and treatment. This review explores the fundamental mechanisms of serine protease inhibitors, summarizes those that have been successfully integrated into clinical practice, and discusses the challenges encountered in their development along with partial solutions. These advancements lay the groundwork for further refinement and innovation in serine protease inhibitor therapeutics.
The Adhesion GPCR ADGRL2/LPHN2 Can Protect Against Cellular and Organismal Dysfunction
Authors: Jakobs Philipp , Rafflenbeul Anne , Post Willem Berend , Ale-Agha Niloofar , Groß Victoria Elisabeth , Pick Stephanie , Dolata Sascha , Cox Fiona F. , von Ameln Florian , Eckermann Olaf , Altschmied Joachim , Prömel Simone , Haendeler Judith
Published: November 2024

The most common trigger of sepsis and septic shock is bacterial lipopolysaccharide (LPS). Endothelial cells are among the first to encounter LPS directly. Generally, their function is closely linked to active endothelial NO Synthase (eNOS), which is significantly reduced under septic conditions. LPS treatment of endothelial cells leads to their activation and apoptosis, resulting in loss of integrity and vascular leakage, a hallmark of septic shock. Hence, therapies that prevent endothelial leakage or restore the endothelial barrier would be invaluable for patients. Adhesion GPCRs (aGPCRs) have been largely overlooked in this context, although particularly one of them, ADGRL2/LPHN2, has been implicated in endothelial barrier function. Our study shows that overexpression of ADGRL2 protects endothelial cells from LPS-induced activation, apoptosis, and impaired migration. Mechanistically, ADGRL2 preserves eNOS activity by shifting its binding from Caveolin-1 to Heat Shock Protein 90. Furthermore, ADGRL2 enhances antioxidative responses by increasing NRF2 activity. Notably, we found that this function may be evolutionarily conserved. In the absence of lat-2, a homolog of ADGRL2 in Caenorhabditis elegans, worms show higher ROS levels and altered stress response gene expression. Additionally, lat-2 mutants have a significantly reduced lifespan, altogether indicating a protective role of ADGRL2 against oxidative stress across species.
The Genetic Basis of Non-Contact Soft Tissue Injuries-Are There Practical Applications of Genetic Knowledge?
Authors: Borzemska Beata , Cięszczyk Paweł , Żekanowski Cezary
Published: November 2024

Physical activity increases the risk of non-contact injuries, mainly affecting muscles, tendons, and ligaments. Genetic factors are recognized as contributing to susceptibility to different types of soft tissue injuries, making this broad condition a complicated multifactorial entity. Understanding genetic predisposition seems to offer the potential for personalized injury prevention and improved recovery strategies. The candidate gene analysis approach used so far, has often yielded inconclusive results. This manuscript reviews the most commonly studied genetic variants in genes involved in the musculoskeletal system’s structure and recovery processes (ACTN3, ACE, CKM, MLCK, AMPD1, IGF2, IL6, TNFα, CCL2, COL1A1, COL5A1, MMP3, and TNC). Referring to the literature, it was highlighted that single-gene analyses provide limited insight. On the other hand, novel genetic testing methods identify numerous variants of uncertain physiological relevance. Distinguishing between functionally important variants, modifying variants, and the thousands of irrelevant variants requires advanced bioinformatics methods and basic multiomics research to identify the key biological pathways contributing to injury susceptibility. Tools like the Total Genotype Score (TGS) and Polygenic Risk Score (PRS) offer a more holistic view by assessing the combined effect of multiple variants. However, these methods, while useful in research, lack clinical applicability. In conclusion, it is too early to determine the clinical implications of genetic variability as a tool for improving well-established training and injury prevention methods, as the predictive power of genetic testing for injury predisposition is currently low.
Decoding Motor Skills: Video Analysis Unveils Age-Specific Patterns in Childhood and Adolescent Movement
Authors: Russo Luca , Micozzi Massimiliano , Racil Ghazi , Larion Alin , Lupu Elena , Padulo Johnny , Migliaccio Gian Mario
Published: November 2024

Motor skill development is crucial in human growth, evolving with the maturation of the nervous and musculoskeletal systems. Quantifying these skills, especially coordinative abilities, remains challenging. This study aimed to assess the performance of five motor tasks in children and adolescents using high-speed video analysis, providing data for movement and health professionals. Seventy-two volunteers were divided into three age groups: 27 first-grade primary school students (19 males and 8 females, aged 6.5 ± 0.5 years), 35 fourth-grade primary school students (16 males and 19 females, aged 9.2 ± 0.4 years), and 28 s-year middle school students (16 males and 12 females, aged 13.0 ± 0.3 years). Participants performed five motor tasks: standing long jump, running long jump, stationary ball throw, running ball throw, and sprint running. Each task was recorded at 120 frames per second and analyzed using specialized software to measure linear and angular kinematic parameters. Quantitative measurements were taken in the sagittal plane, while qualitative observations were made using a dichotomous approach. Statistical analysis was performed using the Kruskal–Wallis and Mann–Whitney tests with Bonferroni correction. Significant differences were observed across age groups in various parameters. In the standing long jump, older participants exhibited a longer time between initial movement and maximum loading. The running long jump revealed differences in the take-off angle, with fourth-grade students performing the best. Ball-throwing tests indicated improvements in the release angle with age, particularly in females. Sprint running demonstrated the expected improvements in time and stride length with age. Gender differences were notable in fourth-grade students during the running long jump, with females showing greater knee flexion, while males achieved better take-off angles. Video analysis effectively identified age-related and gender-specific differences in motor skill performance. The main differences were measured between first-grade primary school and second-year middle school students while gender differences were limited to all age groups. This method provides valuable insights into motor development trajectories and can be used by professionals to objectively assess and monitor the technical aspects of motor skills across different age groups.
Comprehensive Safety Assessment of Lacticaseibacillus paracasei subsp. paracasei NTU 101 Through Integrated Genotypic and Phenotypic Analysis
Authors: Chen Chieh-Ting , Chao Wen-Yu , Lin Chih-Hui , Shih Tsung-Wei , Pan Tzu-Ming
Published: November 2024

Probiotics, as defined by the World Health Organization, are live microorganisms that, when consumed in sufficient quantities, provide health benefits to the host. Although some countries have approved specific probiotic species for use in food, safety concerns may still arise with individual strains. Lacticaseibacillus paracasei subsp. paracasei NTU 101 (NTU 101), isolated from the gut of healthy infants, has demonstrated various probiotic effects and shown safety in a prior 28-day animal feeding study. To further verify its safety and mitigate potential risks, we performed a comprehensive genotypic and phenotypic safety evaluation in accordance with the European Food Safety Authority guidelines for safety assessment through whole genome sequencing and related literature. In this research, minimum inhibitory concentration testing identified NTU 101’s resistance to chloramphenicol; however, subsequent gene analysis confirmed no associated risk of resistance. Assessments of safety, including biogenic amine content, hemolytic activity, mucin degradation, and D-lactic acid production, indicated a low level of risk. Additionally, a repeated-dose 90-day oral toxicity study in Sprague-Dawley rats revealed no toxicity at a dose of 2000 mg/kg body weight, further supporting the strain’s safety for consumption. Based on these comprehensive analyses, NTU 101 is considered safe for regular consumption as a health supplement.
Comparative Analysis of Two Soybean Cultivars Revealed Tolerance Mechanisms Underlying Soybean Adaptation to Flooding
Authors: Yu Xiaobo , An Jiangang , Liang Jianqiu , Yang Wenying , Zeng Zhaoqiong , Zhang Mingrong , Wu Haiying , Liu Sichen , Cao Xiaoning
Published: November 2024

Flooding stress poses a significant challenge to soybean cultivation, impacting plant growth, development, and ultimately yield. In this study, we investigated the responses of two distinct soybean cultivars: flooding-tolerant Nanxiadou 38 (ND38) and flooding-sensitive Nanxiadou 45 (ND45). To achieve this, healthy seedlings were cultivated with the water surface consistently maintained at 5 cm above the soil surface. Our objective was to elucidate the physiological and molecular adaptations of the two cultivars. Under flooding stress, seedlings of both cultivars exhibited significant dwarfing and a notable decrease in root length. While there were no significant differences in the dry weight of aboveground shoots, the dry weight of underground shoots in ND38 was strikingly decreased following flooding. Additionally, total chlorophyll content decreased significantly following flooding stress, indicating impaired photosynthetic performance of the cultivars. Moreover, malondialdehyde (MDA) levels increased significantly after flooding, particularly in the ND45 cultivar, suggesting heightened oxidative stress. Expression analysis of methylation and demethylation genes indicated that MET1 and DME play crucial roles in response to flooding stress in soybeans. Meanwhile, analysis of the hemoglobin family (GLBs), aquaporin family (AQPs), glycolytic pathway-related genes, and NAC transcription factor-related genes identified GLB1-1 and GLB1-2, GLB2-2, PIP2-6, PIP2-7, TIP2-2, TIP4-1, TIP5-1, Gm02G222400 (fructose-bisphosphate aldolase), Gm19G017200 (glucose-6-phosphate isomerase), and Gm04G213900 (alcohol dehydrogenase 1) as key contributors to flooding tolerance in both soybean cultivars. These findings provide crucial insights into the physiological and molecular mechanisms underlying flooding tolerance in soybeans, which could guide future molecular breeding strategies for the development of flooding-tolerant soybean cultivars.
A Novel Input Schematization Method for Coastal Flooding Early Warning Systems Incorporating Climate Change Impacts
Authors: Papadimitriou Andreas G. , Metallinos Anastasios S. , Chondros Michalis K. , Tsoukala Vasiliki K.
Published: November 2024

Coastal flooding poses a significant threat to coastal communities, adversely affecting both safety and economic stability. This threat is exacerbated by factors such as sea level rise, rapid urbanization, and inadequate coastal infrastructure, as noted in recent climate change reports. Early warning systems (EWSs) have proven to be effective tools in coastal planning and management, offering a high cost-to-benefit ratio. Recent advancements have integrated operational numerical models with machine learning techniques to develop near-real-time EWSs, leveraging data obtained from reputable databases that provide reliable hourly sea-state and sea level data. Despite these advancements, a stepwise methodology for selecting representative events, akin to wave input reduction methods used in morphological modeling, remains undeveloped. Moreover, existing methodologies often overlook the significance of compound extreme events and their potential increased occurrence under climate change projections. This research addresses these gaps by introducing a novel input schematization method that combines efficient hydrodynamic modeling with clustering algorithms. The proposed methodοlogy, implemented in the coastal area of Pyrgos, Greece, aims to select an optimal number of representative sea-state and water level combinations to develop accurate EWSs for coastal flooding risk prediction. A key innovation of this methodology is the incorporation of weights in the clustering algorithm to ensure adequate representation of extreme compound events, also taking into account projections for future climate scenarios. This approach aims to enhance the accuracy and reliability of coastal flooding EWSs, ultimately improving the resilience of coastal communities against imminent flooding threats.
Critical Analysis of Tools for Measuring Recovery-Oriented Practice in Mental Health Facilities: A Scoping Review
Authors: Garrido-Cervera Josè Antonio , Ruiz-Granados María Isabel , Cuesta-Vargas Antonio Ignacio , Sánchez-Guarnido Antonio José
Published: November 2024

Background: To implement recovery-oriented practice, it is important to have instruments capable of evaluating such practice. A number of different questionnaires have been developed in recent years which measure recovery orientation in mental health services. Objective: To identify and analyze patient-reported experience measures (PREMs) reported in the literature that are related to recovery-oriented practice in mental health services. Methodology: This study followed the Joanna Briggs Institute methodology for scoping reviews. Searches were carried out in the Web of Science, CINAHL, Medline (via Pubmed), and SCOPUS databases and in grey literature repositories (Google Scholar, Opengrey, Dart-Europe, Teseo). Papers on recovery services for adults suffering from mental disorders (MDs) were included. Those focusing on addiction and intellectual disability care services were excluded. Results: Sixteen papers met the inclusion criteria. The selected PREMs mainly identified recovery-oriented systems, treatment, community integration and support as the dimensions addressed most frequently in questionnaires. The average number of items included in the questionnaires was found to be 54. With regard to psychometric properties, 62% of the papers evaluated reliability (internal consistency) and 56% provided some kind of evidence of validity. Conclusions: This review aims to give an overview of the existing instruments in the literature and to highlight the characteristics of each one of them. Several different PREMs exist which evaluate recovery-oriented practice. No instrument currently exists which could be described as a benchmark tool, but there are quite a few with good psychometric properties capable of producing data that are useful when evaluating clinical services.
Optimizing Chronotherapy in Psychiatric Care: The Impact of Circadian Rhythms on Medication Timing and Efficacy
Authors: Colita Cezar-Ivan , Hermann Dirk M. , Filfan Madalina , Colita Daniela , Doepnner Thorsten R. , Tica Oana , Glavan Daniela , Popa-Wagner Aurel
Published: November 2024

In many medical settings, medications are typically administered in the morning or evening, aligning with patients’ daily routines. This practice does not stem from chronotherapy, which involves scheduling drug administration to enhance its effectiveness, but rather from the way clinical operations are structured. The timing of drug administration can significantly affect a medication’s effectiveness and side effects, with the impact varying by up to ten times based on circadian rhythms. Disorders such as major depression, bipolar disorder, and schizophrenia are linked to disruptions in these rhythms. Recent studies have found that circadian dysfunctions, including genetic and neurohumoral changes, underlie many psychiatric conditions. Issues such as an altered glucocorticoid rhythm due to impaired HPA axis function, disturbed melatonin balance, and sleep disturbances have been noted in psychotic disorders. Furthermore, mood disorders have been associated with changes in the expression of circadian rhythm genes such as Clock, Bmal1, and Per. Considering that the absorption, biodistribution, effects on target organs, half-life, metabolism, and elimination of drugs are all influenced by the body’s circadian rhythms, this narrative review explores the optimal timing of medication administration to maximize efficacy and minimize side effects in the treatment of psychiatric disorders. By closely monitoring circadian variations in cortisol, melatonin, and key clock genes, as well as by deepening our understanding of the metabolisms and pharmacokinetics of antipsychotic medications, we propose a chronotherapy approach for psychiatric patients that could significantly enhance patient care.
Correlation Between the Anticorrosive Performance of Protective Coatings Under Neutral Salt Spray Testing and Outdoor Atmospheric and Immersion Exposure
Authors: Schmid Matthias J.
Published: November 2024

Anticorrosive organic coatings are usually tested with accelerated weathering methods to assess their anticorrosive performance. The results of lab testing often do not correlate well with results from field testing, which resembles the conditions of actual use more closely. We tested the correlation of the neutral salt spray test (NSS, ISO 9227) and tests for mechanical properties and a 5-year field exposure in four different locations in the atmospheric zone, splash zone and immersion zone using 19 organic coatings for hydraulic steelwork. No correlation was found between the anticorrosive performance under outdoor exposure and the mechanical properties of a coating. The NSS test showed a positive correlation with the results in the field in 6 of 12 cases. For the fresh water testing location in Trier, the correlation proved to be very good. The biggest difference between lab and field testing was observed for zinc-primer-free coatings, which passed in the lab testing and failed in the outdoor testing. This study shows that the NSS test correlates with outdoor exposure only in some cases on a statistically significant level, but the results of NSS testing can be useful in approval testing for protective coating systems using predefined pass/fail criteria.