bluuebunny/crossref_metadata_2025 huggingface dataset:
Host Tree and Geography Induce Metabolic Shifts in the Epiphytic Liverwort Radula complanata
Authors: Blatt-Janmaat Kaitlyn L. , Neumann Steffen , Ziegler Jörg , Peters Kristian
Published: January 2023

Bryophytes are prolific producers of unique, specialized metabolites that are not found in other plants. As many of these unique natural products are potentially interesting, for example, pharmacological use, variations in the production regarding ecological or environmental conditions have not often been investigated. Here, we investigate metabolic shifts in the epiphytic Radula complanata L. (Dumort) with regard to different environmental conditions and the type of phorophyte (host tree). Plant material was harvested from three different locations in Sweden, Germany, and Canada and subjected to untargeted liquid chromatography high-resolution mass-spectrometry (UPLC/ESI-QTOF-MS) and data-dependent acquisition (DDA-MS). Using multivariate statistics, variable selection methods, in silico compound identification, and compound classification, a large amount of variation (39%) in the metabolite profiles was attributed to the type of host tree and 25% to differences in environmental conditions. We identified 55 compounds to vary significantly depending on the host tree (36 on the family level) and 23 compounds to characterize R. complanata in different environments. Taken together, we found metabolic shifts mainly in primary metabolites that were associated with the drought response to different humidity levels. The metabolic shifts were highly specific to the host tree, including mostly specialized metabolites suggesting high levels of ecological interaction. As R. complanata is a widely distributed generalist species, we found it to flexibly adapt its metabolome according to different conditions. We found metabolic composition to also mirror the constitution of the habitat, which makes it interesting for conservation measures.
Light-Induced Flavonoid Biosynthesis in Sinopodophyllum hexandrum with High-Altitude Adaptation
Authors: Zhao Qiaozhu , Dong Miaoyin , Li Mengfei , Jin Ling , Paré Paul W.
Published: January 2023

Sinopodophyllum hexandrum is a perennial alpine herb producing the anti-cancer metabolite podophyllotoxin (PPT). Although the adaptation of S. hexandrum to high altitudes has been demonstrated and the effects of temperature, precipitation, and UV-B light on plant growth and metabolite accumulation have been studied, knowledge on the role of flavonoid biosynthesis in adapting to high altitudes is limited. In this study, light intensity, amount and type of flavonoids, and differentially expressed proteins (DEPs) and genes (DEGs) at 2300 and 3300 m were analyzed by HPLC, proteomic, transcriptomic, and qRT-PCR analysis. We found that higher light intensity correlated with greater flavonoid, flavonol, and anthocyanin content as well as higher anthocyanin to total flavonoid and flavonol ratios observed at the higher altitude. Based on proteomic and transcriptomic analyses, nine DEPs and 41 DEGs were identified to be involved in flavonoid biosynthesis and light response at 3300 m. The relative expression of nine genes (PAL, CHS1, IFRL, ANS, MYB4, BHLH137, CYP6, PPO1, and ABCB19) involved in flavonoid biosynthesis and seven genes (HSP18.1, HSP70, UBC4, ERF5, ERF9, APX3, and EX2) involved in light stress were observed to be up-regulated at 3300 m compared with 2300 m. These findings indicate that light intensity may play a regulatory role in enhancing flavonoid accumulation that allows S. hexandrum to adapt to elevated-altitude coupled with high light intensity.
(1′S)-1′-Acetoxyeugenol Acetate Enhances Glucose-Stimulated Insulin Secretion
Authors: Lee Dahae , Son So-Ri , Qi Yutong , Kang Ki Sung , Jang Dae Sik
Published: January 2023

Alpinia galanga have been widely used as spice or traditional medicine in East Asia, commonly known as Thai ginger. In the present study, seven major phenylpropanoids, (±)-1′-hydoxychavicol acetate (1; HCA), (1′S)-1′-acetoxychavicol acetate (2; ACA), (1′S)-1′-acetoxyeugenol acetate (3; AEA), eugenyl acetate (4), trans-p-coumaraldehyde (5), trans-p-acetoxycinnamyl alcohol (6), and trans-p-coumaryl diacetate (7), were isolated from the 95% EtOH and hot water extracts of the rhizomes of A. galanga by chromatographic method. Phenylpropanoids 1–7 were evaluated for glucose-stimulated insulin secretion (GSIS) effect and α-glucosidase inhibitory activity. Phenylpropanoids 1–4 increase GSIS effect without cytotoxicity in rat INS-1 pancreatic β-cells. In addition, INS-1 cells were treated with AEA (3) to determine a plausible mechanism of β-cell function and insulin secretion through determining the activation of insulin receptor substrate-2 (IRS-2), phosphatidylinositol 3-kinase (PI3K), Akt, and pancreatic and duodenal homeobox-1 (PDX-1). Upon treatment with AEA (3), INS-1 cells showed an increase in these protein expressions. Meanwhile, AEA (3) exhibited α-glucosidase inhibitory activity. On the basis of the above findings, we suggest AEA (3) as a potential antidiabetic agent.
Spatio-Temporal Distribution Characteristics and Driving Factors of Main Grain Crop Water Productivity in the Yellow River Basin
Authors: Zhang Yan , Wang Feiyu , Du Zhenjie , Dou Ming , Liang Zhijie , Gao Yun , Li Ping
Published: January 2023

To reveal the relationship between agricultural water resource consumption and grain production in the Yellow River Basin, the irrigation water productivity (WPI), crop water productivity (WPC), total inflow water productivity (WPT), and eleven influencing factors were selected. The spatial and temporal distribution characteristics and driving factors of water productivity of the main crops in the Yellow River Basin were analyzed with the spatial autocorrelation analysis, grey correlation analysis, sensitivity analysis, and relative contribution rate. The results showed that the minimum mean values of WPI, WPC, and WPT were 0.22, 0.35, and 0.18 kg/m3 in Qinghai, respectively, the maximum mean value of WPI was 2.11 kg/m3 in Henan, and the maximum mean values of WPC and WPT were 0.71 and 0.61 kg/m3 in Shandong, respectively. The changing trends in WPI and WPT in Qinghai and in WPC in Shandong were insignificant, whereas the WPI, WPC, and WPT in other provinces showed a significant increasing trend. Water productivity displayed a certain spatial clustering feature in the Yellow River Basin in different years, such as a high-high (H-H) aggregation in Henan in 2005, and an H-H aggregation in Shanxi in 2015 for WPI. The water productivity had a significant positive correlation with the consumption of chemical fertilizer with a 100% effective component (CFCEC), effective irrigated area (EIA), plastic film used for agriculture (PFUA), and total power of agricultural machinery (AMTP), while it had a significant negative correlation with the persons engaged in rural areas (PERA). There was a large grey correlation degree between the water productivity and the average annual precipitation (AAP), CFCEC, PFUA, consumption of chemical pesticides (CFC), and AMTP in the Yellow River Basin, but their sensitivity was relatively small. The main driving factors were EIA (8.98%), agricultural water (AW, 15.55%), AMTP (12.64%), CFCEC (12.06%), and CPC (9.77%) for WPI; AMTP (16.46%), CFCEC (13.25%), average annual evaporation (AAE, 12.94%), EIA (10.49%), and PERA (10.19%) for WPC; and EIA (14.26%), AMTP (13.38%), AAP (12.30%), CFCEC (10.49%), and PFUA (9.69%) for WPT in the Yellow River Basin. The results can provide support for improving the utilization efficiency of agricultural water resources, optimizing the allocation of water resources, and implementing high-quality agricultural developments in the Yellow River Basin.
Impact of Chill and Heat Exposures under Diverse Climatic Conditions on Peach and Nectarine Flowering Phenology
Authors: Drogoudi Pavlina , Cantín Celia M. , Brandi Federica , Butcaru Ana , Cos-Terrer José , Cutuli Marcello , Foschi Stefano , Galindo Alejandro , García-Brunton Jesus , Luedeling Eike , Moreno María Angeles , Nari Davide , Pantelidis Georgios , Reig Gemma , Roera Valentina , Ruesch Julien , Stanica Florin , Giovannini Daniela
Published: January 2023

The present study aims to generalize cultivar-specific tree phenology responses to winter and spring temperatures and assess the effectiveness of the Tabuenca test and various chill and heat accumulation models in predicting bloom dates for a wide range of climatic conditions and years. To this end, we estimated the dates of rest completion and blooming and correlated them with observed bloom dates for 14 peach and nectarine cultivars that were evaluated in 11 locations across Europe (Greece, France, Italy, Romania and Spain), within the EUFRIN cultivar testing trial network. Chill accumulation varied considerably among the studied sites, ranging from 45 Chill Portions (CP) in Murcia-Torre Pacheco (Spain) to 97–98 CP in Cuneo (Italy) and Bucharest (Romania). Rest completion occurred latest or was not achieved at all for some cultivars in the southern sites in Murcia. Dormancy release happened earliest in Bucharest and Cuneo, sites where heat accumulation had a strong influence on the regulation of bloom time. Blooming occurred earliest in the moderately cold regions of Lleida (Spain) and Bellegarde (France), and 7–11 days later in the warmer locations of Rome (Italy) and Naoussa (Greece), suggesting that bloom timing is strongly influenced by delayed rest completion in these locations. The Dynamic Model resulted in both more homogeneous chill accumulation across years and better predictions of bloom dates, compared with the Utah, Positive Utah and Chilling Hours models. Prediction of bloom dates was less successful for low-chill cultivars than for medium- and high-chill cultivars. Further climatic and experimental data are needed to make estimates of the climatic needs of peach cultivars more robust and to generate reliable advice for enhancing the resilience of peach production under varying and changing climatic conditions.
Improvement of Water and Nitrogen Use Efficiencies by Alternative Cropping Systems Based on a Model Approach
Authors: Han Le , Li Yunrui , Hou Yonghao , Liang Hao , Feng Puyu , Hu Kelin
Published: January 2023

The conventional double cropping system of winter wheat and summer maize (WW-SUM) in the North China Plain (NCP) consumes a large amount of water and chemical fertilizer, threatening the sustainable development of agriculture in this region. This study was based on a three-year field experiment of different cropping systems (2H1Y—two harvests in one year; 3H2Y—three harvests in two years; and 1H1Y—one harvest in one year). The 2H1Y system had three irrigation–fertilization practices (FP—farmer’s practice; RI—reduced input; and WQ—Wuqiao pattern in Wuqiao County, Hebei Province). A soil–crop system model (WHCNS—soil water heat carbon nitrogen simulator) was used to quantify the effects of different cropping systems on water and nitrogen use efficiencies (WUE and NUE, respectively), and to explore the trade-offs between crop yields and environmental impacts. The results showed that annual yield, water consumption, and the WUE of 2H1Y were higher than those of the 3H2Y and 1H1Y systems. However, local precipitation during the period of crop growth could only meet 65%, 76%, and 91% of total water consumption for the 2H1Y, 3H2Y and 1H1Y systems, respectively. Nearly 65% of irrigation water (groundwater) was used in the period of wheat growth that contributed to almost 40% of the annual yield. Among the three patterns of the 2H1Y system, the order of the WUE was 2H1Y_RI > 2H1Y_WQ > 2H1Y_FP. Compared to 2H1Y_FP, the total fertilizer N application rates in 2H1Y_WQ, 2H1Y_RI, and 3H2Y were reduced by 25%, 65%, and 74%, respectively. The 3H2Y system had the highest NUE of 34.3 kg kg−1, 54% greater than the 2H1Y_FP system (22.2 kg kg−1). Moreover, the 3H2Y system obviously reduced nitrate leaching and gaseous N loss when compared with the other two systems. The order of total N loss of different cropping systems was 2H1Y (261 kg N ha−1) > 1H1Y (78 kg N ha−1) > 3H2Y (70 kg N ha−1). Considering the agronomic and environmental effects as well as economic benefits, the 3H2Y cropping system with optimal irrigation and fertilization would be a promising cropping system in the NCP that could achieve the balance between crop yield and the sustainable use of groundwater and N fertilizer.
Influence of Foliar Application of Hydrogen Peroxide on Gas Exchange, Photochemical Efficiency, and Growth of Soursop under Salt Stress
Authors: Capitulino Jessica Dayanne , Lima Geovani Soares de , Azevedo Carlos Alberto Vieira de , Silva André Alisson Rodrigues da , Arruda Thiago Filipe de Lima , Soares Lauriane Almeida dos Anjos , Gheyi Hans Raj , Dantas Fernandes Pedro , Sobral de Farias Maria Sallydelândia , Silva Francisco de Assis da , Dias Mirandy dos Santos
Published: January 2023

Hydrogen peroxide at low concentrations has been used as a salt stress attenuator because it induces a positive response in the antioxidant system of plants. This study aimed to assess the gas exchange, quantum yield, and development of soursop plants cv. Morada Nova grown with saline water irrigation and foliar hydrogen peroxide application. The experiment was carried out under greenhouse conditions using a randomized block design in a 4 × 4 factorial scheme corresponding to four levels of electrical conductivity of irrigation water, ECw (0.8, 1.6, 2.4, and 3.2 dS m−1), and four doses of hydrogen peroxide, H2O2 (0, 10, 20, and 30 μM), with three replicates. The use of irrigation water with electrical conductivity above 0.8 dS m−1 inhibited stomatal conductance, internal CO2 concentration, transpiration, maximum fluorescence, crown height, and vegetative vigor index of the Morada Nova cultivar of soursop. Compared to untreated plants, the hydrogen peroxide concentration of 30 μM resulted in greater stomatal conductance. Water salinity of 0.8 dS m−1 with hydrogen peroxide concentrations of 16 and 13 μM resulted in the highest variable fluorescence and quantum efficiency of photosystem II, respectively, of soursop plants cv. Morada Nova at 210 days after transplantation.
Pulsed Plasma Accelerator
Authors: Karimov Alexander , Terekhov Svyatoslav , Yamschikov Vladimir
Published: January 2023

In this paper, we consider the acceleration of plasma fluxes in crossed electromagnetic fields. The possible technical approach to a prospective plasma accelerator is discussed. A simple hydrodynamic model describing the dynamics of the plasma ring in these fields is proposed. Based on this model, the estimations of basic characteristics for the accelerated flux are calculated for typical experimental conditions.
CVD Deposited Epoxy Copolymers as Protective Coatings for Optical Surfaces
Authors: Karabıyık Merve , Cihanoğlu Gizem , Ebil Özgenç
Published: January 2023

Copolymer thin films of glycidyl methacrylate (GMA), ethylene glycol dimethacrylate (EGDMA) and 2,4,6,8-tetramethyl-2,4,6,8-tetravinylcyclotetrasiloxane (V4D4) were synthesized via initiated chemical vapor deposition (iCVD) as protective coatings for optical surfaces. Chemical durability in various solvents, corrosion resistance, adhesion to substrate, thermal resistance and optical transmittance of the films were evaluated. Crosslinked thin films exhibited high chemical resistance to strong organic solvents and excellent adhesion to substrates. Poly(GMA-co-EGDMA) and poly(GMA-co-V4D4) copolymers demonstrated protection against water (<1% thickness loss), high salt resistance (<1.5% thickness loss), and high optical transparency (~90% in visible spectrum) making them ideal coating materials for optical surfaces. Combining increased mechanical properties of GMA and chemical durability V4D4, the iCVD process provides a fast and low-cost alternative for the fabrication of protective coatings.
Color Stability of Resin Cements after Water Aging
Authors: Mazzitelli Claudia , Paolone Gaetano , Sabbagh Joseph , Scotti Nicola , Vichi Alessandro
Published: January 2023

The color stability of resin cements plays a key role in the achievement of esthetically-pleasant restorations. Resin luting materials can be mainly divided into two main classes: adhesive (relying on previous application of adhesive systems) or self-adhesive (also known as one-step cements). The different chemical compositions determine their physio-mechanical characteristics which, in turns, influence their color stability. To evaluate the color variations of different dual-cured resin cements after water aging, 80 disc-shaped specimens (15 mm in diameter and 1.2 mm thick) were obtained from the following resin cements (n = 10): (1) Maxcem Elite Universal, MCU (Kerr); (2) RelyX Universal, RXU (3M); (3) Calibra Ceram, CAL (Dentsply); (4) Multilink, MUL (Ivoclar-Vivadent); (5) Panavia V5, PAN (Kuraray); (6) Calibra Universal, CUN (Dentsply); (7) SpeedCEM Plus, SCP (Ivoclar); and (8) Panavia SA, PSA (Kuraray). After light-polymerization, the specimens were measured with a spectrophotometer and CIELab* values were recorded. The specimens were then placed in a digitally controlled thermostatic water bath at 60° for 30 days and afterwards the color measurements were repeated. Color differences were calculated for each specimen before and after water-aging procedures with ΔEab formula and the data were statistically analyzed (p < 0.05). The type of cement statistically influenced the ΔEab (p < 0.05), with MCU showing the lowest color variations (4.3 ± 0.7) whereas RXU and PSA the highest (16.9 ± 1.6 and 16.8 ± 1.2, respectively). No differences were observed between CAL, CUN and SCP (p = 0.05). Color stability is related to the chemical composition of the resinous luting materials, thus material dependent.
Optimization Preparation and Evaluation of Chitosan Grafted Norfloxacin as a Hemostatic Sponge
Authors: Cheng Yu , Yang Qian , Wang Jiyuan , Hu Zhang , Li Chengpeng , Zhong Saiyi , Huang Na
Published: January 2023

Considering the great harm to the human body caused by severe and massive bleeding, in this study, chitosan-grafted norfloxacin (CTS-NF) composites were prepared with chitosan (CTS) and norfloxacin (NF) as raw materials by a 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide-mediated coupling method to solve the limitations of slow hemostatic and poor anti-infective effects of current dressings on the market. The effects of the mass ratio of CTS to NF (MCTS/MNF), reaction temperature T and reaction time t on the grafting rate (η%) of the products were investigated through single factor tests. The preparation process was optimized with the η% as an evaluation index by means of the Box–Behnken test design and response surface analysis. The antimicrobial activity was evaluated by inhibition zone assay, and the hemostatic activity of the prepared composites was evaluated in vitro and in vivo. The results suggested that the optimum preparation conditions were the mass ratio of CTS to NF (MCTS/MNF) 5:3, reaction temperature 65 °C, and reaction time 4 h. Under this condition, the η% of CTS-NF was 45.5%. The CTS-NF composites displayed significant antimicrobial activities. Moreover, in vitro hemostasis results revealed that the CTS-NF composite had a lower blood clotting index and absorbed red blood cells to promote aggregation. In vivo ear and live hemostasis, the CTS-NF groups showed short hemostatic time (49.75 ± 3.32 s and 50.00 ± 7.21 s) and more blood loss (0.07 ± 0.010 g and 0.075 ± 0.013 g). The results showed that CTS-NF reduced the bleeding time and volume, exhibiting a significant coagulation effect. Therefore, the CTS-NF sponge is expected to be a new, effective hemostatic and antibacterial material in the future.
Entrapment of Acridine Orange in Metakaolin-Based Geopolymer: A Feasibility Study
Authors: D’Angelo Antonio , Vertuccio Luigi , Leonelli Cristina , Alzeer Mohammad I. M. , Catauro Michelina
Published: January 2023

Few studies have explored the immobilization of organic macromolecules within the geopolymer matrix, and some have found their chemical instability in the highly alkaline geopolymerization media. The present work reports on the feasibility of encapsulating the potentially toxic acridine orange (AO) dye in a metakaolin based geopolymer while maintaining its structural integrity. The proper structural, chemical, and mechanical stabilities of the final products were ascertained using Fourier-transform infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM), X-ray diffraction (XRD), thermogravimetric (TGA/DTG), and mechanical analyses, whereas the dye integrity and its stability inside the geopolymer were investigated by the UV-Vis analysis. In addition, the antimicrobial activity was investigated. The FT-IR and XRD analyses confirmed the geopolymerization occurrence, whereas the TGA/DTG and mechanical (compressive and flexural) strength revealed that the addition of 0.31% (AO mg/ sodium silicate L) of AO to the fresh paste did not affect the thermal stability and the mechanical properties (above 6 MPa in flexural strength and above 20 MPa for compressive strength) of the hardened product. UV-Vis spectroscopy revealed that the dye did not undergo chemical degradation nor was it released from the geopolymer matrix. The results reported herein provide a useful approach for the safe removal of toxic macromolecules by means of encapsulation within the geopolymer matrix.
Two-Stage Bio-Hydrogen and Polyhydroxyalkanoate Production: Upcycling of Spent Coffee Grounds
Authors: Kang Beom-Jung , Jeon Jong-Min , Bhatia Shashi Kant , Kim Do-Hyung , Yang Yung-Hun , Jung Sangwon , Yoon Jeong-Jun
Published: January 2023

Coffee waste is an abundant biomass that can be converted into high value chemical products, and is used in various renewable biological processes. In this study, oil was extracted from spent coffee grounds (SCGs) and used for polyhydroxyalkanoate (PHA) production through Pseudomonas resinovorans. The oil–extracted SCGs (OESCGs) were hydrolyzed and used for biohydrogen production through Clostridium butyricum DSM10702. The oil extraction yield through n–hexane was 14.4%, which accounted for 97% of the oil present in the SCGs. OESCG hydrolysate (OESCGH) had a sugar concentration of 32.26 g/L, which was 15.4% higher than that of the SCG hydrolysate (SCGH) (27.96 g/L). Hydrogen production using these substrates was 181.19 mL and 136.58 mL in OESCGH and SCGH media, respectively. The consumed sugar concentration was 6.77 g/L in OESCGH and 5.09 g/L in SCGH media. VFA production with OESCGH (3.58 g/L) increased by 40.9% compared with SCGH (2.54 g/L). In addition, in a fed–batch culture using the extracted oil, cell dry weight was 5.4 g/L, PHA was 1.6 g/L, and PHA contents were 29.5% at 24 h.
Utilization of Corncob as an Immobilization Matrix for a Xylanolytic Yeast Strain
Authors: Aftab Maham , Ejaz Uroosa , Pashameah Rami Adel , Fatima Aimen , Syed Jaweria , Ansari Immad , Sohail Muhammad , AlSubhi Samah A. , Alzahrani Eman , El-Bahy Zeinhom M.
Published: January 2023

Immobilization of microbial cells for the production of industrially important enzymes has been reported to offer the advantages of recyclability, higher yields and cost effectiveness. The search for an appropriate matrix that is affordable and easy to prepare is a significant topic in microbial biotechnology. Here, an abundant type of agro-industrial waste—corncob—was utilized as an immobilization matrix for the production of xylanase from an indigenous yeast strain, Saccharomyces cerevisiae MK-157. This is the first report describing xylanase production from immobilized S. cerevisiae. To render the corncob matrix more porous, alkaline pretreatment was undertaken and yeast cells were immobilized on the matrix by cultivating at 30 °C for 48 h in Sabouraud dextrose broth. After incubation, the immobilized matrix was transferred to mineral salt medium containing 1% xylan and incubated at 30 °C for 24 h. Xylanase production was determined in cell-free culture supernatant and the matrix was recycled for up to seven cycles. Moreover, xylanase-mediated saccharification was carried out using sugarcane bagasse as a substrate and the release of reducing sugars was monitored. The results showed that the immobilized yeast produced 4.97 IU mL−1 xylanase in the first production cycle, indicating a >tenfold increase compared to the free cells. Xylanase production further increased to its maximum levels (9.23 IU mL−1) in the fourth production cycle. Nonetheless, the cells retained 100% productivity for up to seven cycles. The volumetric and specific productivity of xylanase were also the highest in the fourth cycle. Scanning electron microscopy images revealed the rough surface of the untreated corncob, which became more porous after alkaline pretreatment. Immobilized yeast cells were also visible on the corncob pieces. The saccharification of a natural resource—sugarcane bagasse—using xylanase preparation yielded 26 mg L−1 of reducing sugars. Therefore, it can be concluded that yeast strains can yield sufficient quantities of xylanase, allowing possible biotechnological applications. Moreover, corncob can serve as a cost-effective matrix for industrially important yeast strains.
Proporties and Synthesis of Biosilver Nanofilms for Antimicrobial Food Packaging
Authors: Baysal Gülay , Demirci Cihat , Özpinar Haydar
Published: January 2023

In this original research, biodegradable corn starch (CS) and wheat gluten (wg)-based silver nanofilms were synthesized and analyzed by using goji berry extract taurine (ta), garlic extract (GC), whey powder (wh), and montmorillonite clay nanoparticles. Antibacterial-corn-starch-based nano films were analyzed by using the methods of high-performance liquid chromatography (HPLC), Fourier Transform infrared spectroscopy (FTIR-ATR), X-ray diffraction (XRD), dynamic and mechanical (DMA) analysis, and scanning electron microscopy (SEM). In addition, the antibacterial resistances of the corn starch nano films against the bacteria Salmonella and Staphylococcus aureus (S. aureus) and Listeria monocytogenes were examined and the migration assays were carried out. The migration analysis results of CS1, CS2, and CS3 nanocomposite films were found as 0.305, 0.297, and 0.297 mg/dm2, respectively. The inhibition zone of CS1, CS2, and CS3 nanocomposite films were found as 1547, 386, and 1884 mm2 against Salmonella bacteria. The results show that silver nanofilms are suitable as packaging films for the production of packaging in milk and dairy products, liquid foods, and acidic foods.
Development of a Method for Peeling Off Paper from Celluloid Pictures for Animation Films
Authors: Kaneko Masahiro , Kim Joon Yang , Ishida Minori , Kawai Mika , Mitsumata Tetsu
Published: January 2023

During the storage of celluloid pictures for animation films over half a century, an interleave paper adhered to acrylic paint. The purpose of this study is to establish a methodology to cleanly remove the paper from the paint. A layered film, a replica of the celluloid pictures, adhered with paper was prepared and immersed in water or ethanol. The effect of these solvents on the peeling behavior was investigated using a peel test. The maximum peel force for the dry layered film in was distributed at ~0.5 N, independently of the peel speed. The peel force was significantly reduced after the layered film was immersed in pure water or ethanol. A morphological observation revealed that the dry paper was peeled off via the cohesive failure of the paper. After the layered film was immersed in pure water, the paper was also peeled off via cohesive failure. The layered film immersed in ethanol was peeled off at the paper/paint interface. To clear the effect of the volume change in the paint on peel behavior, the relative volume was determined via image analysis. The relative volume of paint was 1.56 in pure water and 1.37 in ethanol. It can be considered that the large difference in the volume of paint induces a large shear stress at the paint/paper interface.
Structural Insights into LDPE/UHMWPE Blends Processed by γ-Irradiation
Authors: Zaharescu Traian , Nicula Nicoleta , Râpă Maria , Iordoc Mihai , Tsakiris Violeta , Marinescu Virgil Emanuel
Published: January 2023

Ultra-high-molecular-weight polyethylene (UHMWPE) matrices containing low-density polyethylene (LDPE), hydroxyapatite (HAp) as filler, and rosemary extract (RM) as stabilizer were investigated for their qualification for long-term applications. The significant contributions of the blend components were analyzed, and variations in mechanical properties, oxidation strength, thermal behavior, crystallinity, and wettability were discussed. SEM images of microstructural peculiarities completed the introspective survey. The stability improvement due to the presence of both additives was an increase in the total degradation period of 67% in comparison with an unmodified HDPE/UHMWPE blend when the materials were subjected to a 50 kGy γ-dose. There was growth in activation energies from 121 kJ mol−1 to 139 kJ mol−1 when HAp and rosemary extract delayed oxidation. The exposure of samples to the action of γ-rays was found to be a proper procedure for accomplishing accelerated oxidative degradation. The presence of rosemary extract and HAp powder significantly increased the thermal and oxidation resistances. The calculation of material lifetimes at various temperatures provided meaningful information on the wearability and integrity of the inspected composites.
A Review on Solar Chimneys: From Natural Convection Fundamentals to Thermohydraulic Best-Performance Proposals
Authors: Zamora Blas
Published: January 2023

This work presents an overview of (passive) solar chimney research, from the natural convection fundamentals to the recent progress for achieving thermohydraulic best-performance. Solar chimneys are attractive because they contribute to increasing the efficiency in air conditioning processes for dwellings and buildings, and therefore also aid to reduction in greenhouse gas emissions. A wide number of works dealing with solar chimneys (and Trombe walls or similar) shape designs, as well as with the inclusion of obstacles for disturbing the airflow, is commented in detail. Several numerical simulation procedures used in the literature are specially discussed, and different recommendations are pointed out to be considered for the appropriate numerical simulation of the operating modes of a solar chimney. Investigations aiming for the best performance conditions (for both thermal, and dynamic or ventilation modes) deserve special attention.
Special Issue on “Processing and Conversion of Oil and Gas: Modeling, Control, Simulation and Optimization”
Authors: Assaf Jean Claude , Al Daccache Marina
Published: January 2023

Petroleum is considered the black gold of the earth, but this treasure cannot be utilized without the usage of innovative and advanced technologies for its recovery and conversion [...]
Improving Process Efficiency at Pediatric Hospital Emergency Department Using an Integrated Six-Sigma Simulation Methodology
Authors: Mistarihi Mahmoud Z. , AL-Tahat Mohammad D. , AL-Nimer Saif H.
Published: January 2023

Inadequate staffing and long waiting times in hospital emergency rooms are key concerns that can have a negative impact on patient safety and health, as well as the hospital’s overall performance. The purpose of this paper is to investigate the scope of combining the DMAIC (define, measure, analyze, improve, and control) methodology with discrete event simulation and to explore its successful deployment in the Jordanian healthcare sector. The study discussed in this paper is based on a case study conducted utilizing the DMAIC and simulation technique and its application in reducing waiting time and enhancing overall system efficiency in Jordan’s Princess Rahma hospital’s pediatric emergency department. The study shows improvements in the performance of the process and thus productivity in the emergency department through adapting the combined Six Sigma DES methodology. The cycle time of the process was reduced by 73% of the present value, while simultaneously enhancing the total performance of the emergency department by 83%.
Methods and Advances in the Design, Testing and Development of In Vitro Diagnostic Instruments
Authors: Wang Lei , Xu Wenchang , Wang Biao , Si Xiaonan , Li Shengyu
Published: January 2023

With the continuous improvement of medical testing and instrumentation engineering technologies, the design, testing and development methods of in vitro diagnostic instruments are developing rapidly. In vitro diagnostic instruments are also gradually developing into a class of typical high-end medical equipment. The design of in vitro diagnostic instruments involves a variety of medical diagnostic methods and biochemical, physical and other related technologies, and its development process involves complex system engineering. This paper systematically organizes and summarizes the design, testing and development methods of in vitro diagnostic instruments and their development in recent years, focusing on summarizing the related technologies and core aspects of the R&D process, and analyzes the development trend of the in vitro diagnostic instrument market.
Investigations on Microstructure and Mechanical Properties of AZ91 Alloy Processed by Single Pass Rolling with Varied Rolling Reductions
Authors: Li Yuhua , Xu Qiong , Ma Aibin , Zhang Jin , Shen Yalong , Jiang Jinghua , Jiang Yaqing , Liu Huan
Published: January 2023

In this study, single pass rolling (SPR) with varied rolling reductions was conducted on pre-homogenized AZ91 alloys (H alloys) and ECAP-processed AZ91 alloys (HE alloys). The effects of rolling reduction on the microstructures and mechanical properties of HR alloys (pre-homogenized and rolled) and HER alloys (ECAP-processed and rolled) were investigated. The results showed that the HER alloys possessed much finer microstructures and superior mechanical properties than the HR alloys, which were significantly influenced by the rolling reduction. The microstructures of the HER alloys became bimodal, and the strength and elongation markedly improved with the increase in the rolling reduction. When the rolling reduction reached 70%, the HER alloys acquired a typical bimodal structure, contributing to their excellent comprehensive mechanical properties.
Tail Prediction for Heterogeneous Data Center Clusters
Authors: Malebary Sharaf , Alesawi Sami , Che Hao
Published: January 2023

Service providers need to meet their service level objectives (SLOs) to ensure better client experiences. Predicting tail sojourn times of applications is an essential step to combat long tail latency. Therefore, as an attempt to further unravel the power of our prediction model, new study scenarios for heterogeneous environments will be introduced in this research by using either of two methods: white- or black-box solutions. This research presents several techniques for modeling clusters of inhomogeneous nodes. Those techniques are recognized as heterogeneous fork-join queuing networks (HFJQNs). Moreover, included in the research is a nested-event-based simulation model, borrowing help from multi-core technologies. This model adopts the multiprocessing technique to take part in its design to enable different architectural designs for all computing nodes. This novel implementation of the simulation model is believed to be the next logical step for research studies targeting heterogeneous clusters in addition to the several provided scenarios. Experimental results confirm that even with the existence of such heterogeneous conditions, the tail latency can be predicted at high-load regions with an approximated relative error of less than 15%.
Dough Rheological Properties and Macronutrient Bioavailability of Cereal Products Fortified through Legume Proteins
Authors: Neji Chaima , Semwal Jyoti , Máthé Endre , Sipos Péter
Published: January 2023

Cereal products are regarded as important protein providers, though they could feature poor nutritional quality due to their occasional imbalanced amino acid content. Cereal proteins are low in cysteine or tryptophan, and rich in methionine; however, while their combination with legume proteins makes them nutritionally more comprehensive, such a possibility must be addressed by the cereal processing industry. However, the incorporation of legume protein concentrates and isolates might also influence the functionality and bioavailability of some cereal constituents. Therefore, the objective of the present review is to gain insights into the effects of cereal products incorporated with legume protein isolates/concentrates, knowing that both the cereals and the protein extracts/isolates are complex structural matrices, and besides the final products acceptability they should efficiently promote the health condition of consumers. The combination of legume proteins with cereals will bring about a structural complexity that must harmoniously include proteins, carbohydrates, lipids, polyphenols and dietary fibers to promote the bioaccessibility, bioavailability and bioactivity without cyto- and genotoxicity.
Microscopic Depictions of Vanishing Shampoo Foam Examined by Time-of-Flight Small-Angle Neutron Scattering
Authors: Koizumi Satoshi , Noda Yohei , Inada Takumi , Maeda Tomoki , Yada Shiho , Yoshimura Tomokazu , Shimosegawa Hiroshi , Fujita Hiroya , Yamada Munehiro , Matsue Yukako
Published: January 2023

A novel surfactant of N–dodecanoyl–N–(2-hydroxyethyl)–β–alanine (coded as C12–EtOH–βAla) was synthesized by modifying the methyl group of N–dodecanoyl–N–methyl–β–alanine (coded as C12–Me–βAla). Amino-acid-type surfactants (C12–EtOH–βAla and C12–Me–βAla) are more healthy and environmentally friendly compared to sodium dodecyl sulfate (SDS). To investigate the microstructures of these new surfactants, we employed a method of time-of-flight small-angle neutron scattering (TOF SANS) at a pulsed neutron source, Tokai Japan (J–PARC). The advances in TOF SANS enable simultaneous multiscale observations without changing the detector positions, which is usually necessary for SANS at the reactor or small-angle X-ray scattering. We performed in situ and real-time observations of microstructures of collapsing shampoo foam covering over a wide range of length scales from 100 to 0.1 nm. After starting an air pump, we obtained time-resolved SANS from smaller wave number, small-angle scattering attributed to (1) a single bimolecular layer with a disk shape, (2) micelles in a bimolecular layer, and (3) incoherent scattering due to the hydrogen atoms of surfactants. The micelle in the foam film was the same size as the micelle found in the solution before foaming. The film thickness (~27 nm) was stable for a long time (<3600 s), and we simultaneously found a Newton black film of 6 nm thickness at a long time limit (~1000 s). The incoherent scattering obtained with different contrasts using protonated and deuterated water was crucial to determining the water content in the foam film, which was about 10~5 wt%.
The Effect of Catholic Education on Economic Ideology
Authors: Burrus Robert T. , Echevarria David , Glew David , Jones Adam
Published: January 2023

Religious adherence, church attendance, and economic ideology have long been intertwined. We discuss the compatibility between Catholic doctrine and individual-based free market systems and then examine the effect of Catholic school attendance on an individual’s belief in and support for a capitalist economic system. Using individual level survey data, we find a positive relationship between attendance at Catholic schools and capitalist ideology as measured by an index that contrasts individual versus government action and responsibility in the economy. While attendance at Catholic school is associated with a stronger emphasis on individual, rather than governmental, responsibility in the economy, the effect is driven largely by Catholic high school or college attendance, reflecting an increased exposure to Catholic faith during the formative years of late adolescence.
An Analysis of Volume, Length and Segmentation of Free Fibula Flap in Reconstruction of the Jaws: Investigation of Their Role on Flap Failure
Authors: Di Bartolomeo Mattia , Lusetti Irene Laura , Pinelli Massimo , Negrello Sara , Pellacani Arrigo , Angelini Stefano , Chiarini Luigi , Nocini Riccardo , De Santis Giorgio , Anesi Alexandre
Published: January 2023

Reconstruction of defects of the jaws is mainly performed via free fibula flap. An incidence of 2–21% of overall flap failure is still described. We investigated the roles of volume, length and number of fibula flap segments on flap survival using novel three-dimensional segmentation tools. We also analyzed the role of other possible risk factors. Seventy-one consecutive patients with a follow up of at least three months and who underwent free fibula flap reconstruction in a single center between 2002 and 2022 have been evaluated. A total of 166 fibula segments were analyzed. Malignancies were the main reason of resection (45.1%). In 69% of the cases a reconstruction of the mandible was performed. The flaps were mainly divided in two segments (39%) (range 1–4), with a mean length of 2.52 cm and a mean volume was 3.37 cm3. Total flap failure (TFF) occurred in 12 cases, (16.9%), while partial flap failure (PFF) appeared in 3 patients (4.2%). Volume, length and number of fibula flap segments did not seem to influence flap failure incidence in uni- and multivariate analysis. Reconstruction of the maxilla and use of a recipient vessel different from the facial artery seemed to significantly impact on flap failure. Smoking and previous surgeries showed a higher trend to flap failure, but they did not reach statistical significance. Prospective and multicentric analysis on a wider population should be assessed.
A New Bioreactor to Promote Human Follicular Growth with or without Activin A in Transgender Men
Authors: Jovet Cynthia , Fraison Eloïse , Lornage Jacqueline , Journel Nicolas Morel , Gavoille Antoine , David Laurent , Montembault Alexandra , Fournier Cyrielle , Salle Bruno , Labrune Elsa
Published: January 2023

The aim of the present study was to evaluate the effect of activin A on the activation of in vitro folliculogenesis of human ovarian tissues from transgender men with or without our new compartmented chitosan hydrogel microbioreactor (“three-dimensional (3D)-structure”) enabling a three-dimensional tissue culture. Five fresh ovarian human tissues were cultured in vitro for 20 or 22 days in four groups with 100 ng/mL activin A or without activin A during the last six to eight days of culture, and within a 3D-structure or without the 3D-structure in standard conditions. Follicular density and quality were evaluated, and follicular diameters were measured. Estradiol secretion was quantified. Proliferation and apoptosis through immunostaining were also performed. The proportion of primordial follicles was significantly reduced, and the proportion of primary and secondary follicles was significantly increased in all four groups (p < 0.001). Tertiary follicles were observed in the four culture groups. Activin A supplementation did not significantly affect the follicular density, follicular quality, follicular growth, or estradiol secretion (p > 0.05). The 3D-structure increased the density of primary follicles and decreased the estradiol secretion (p < 0.001). Follicular proliferation was significantly lower in the 3D-structure group compared to the non-3D-structure group (p = 0.008). Regarding follicular apoptosis, it was significantly higher in the activin group compared to the non-activin group (p = 0.006). Activin A did not seem to play a key role in the in vitro folliculogenesis activation in our culture conditions. However, the results may indicate that the 3D-structure could be more physiological and could prevent a detrimental in vitro folliculogenesis flare-up.
Detection of Aquatic Invasive Plants in Wetlands of the Upper Mississippi River from UAV Imagery Using Transfer Learning
Authors: Chaudhuri Gargi , Mishra Niti B.
Published: January 2023

Aquatic invasive plants (AIPs) are a global threat to local biodiversity due to their rapid adaptation to the new environments. Lythrum salicaria, commonly known as purple loosestrife, is a predominant AIP in the upper Midwestern region of the United States and has been designated as a deadly threat to the wetlands of this region. Accurate estimation of its current extent is a top priority, but regular monitoring is limited due to cost-, labor-, and time-intensive field surveys. Therefore, the goal of the present study is to accurately detect purple loosestrife from very high-resolution UAV imagery using deep neural network-based models. As a case study, this study implemented U-Net and LinkNet models with ResNet-152 encoder in the wetlands of the upper Mississippi River situated in La Crosse County, Wisconsin. The results showed that both models produced 88–94% training accuracy and performed better in landscapes that were occupied by smaller, disaggregated, and more equitably distributed purple loosestrife. Furthermore, the study adopted a transfer learning approach to implement a trained purple loosestrife model of the first study site and implemented it for the second study site. The results showed that the pre-trained model implementation generated better accuracy in less than half the time of the original model. Therefore, the transfer learning approach, if adapted efficiently, can be highly beneficial for continuous monitoring of purple loosestrife and strategic planning for application of direct biocontrol measures.
Monitoring Land Cover Change by Leveraging a Dynamic Service-Oriented Computing Model
Authors: Xing Huaqiao , Wang Haihang , Zhang Jinhua , Hou Dongyang
Published: January 2023

Land cover change (LCC) is increasingly affecting global climate change, energy cycle, carbon cycle, and water cycle, with far-reaching consequences to human well-being. Web service-based online change detection applications have bloomed over the past decade for monitoring land cover change. Currently, massive processing services and data services have been published and used over the internet. However, few studies consider both service integration and resource sharing in land cover domain, making end-users rarely able to acquire the LCC information timely. The behavior interaction between services is also growing more complex due to the increasing use of web service composition technology, making it challenging for static web services to provide collaboration and matching between diverse web services. To address the above challenges, a Dynamic Service Computing Model (DSCM) was proposed for monitoring LCC. Three dynamic computation strategies were proposed according to different users’ requirements of change detection. WMS-LCC was first developed by extending the existing WMS for ready-use LCC data access. Spatial relation-based LCC data integration was then proposed for extracting LCC information based on multi-temporal land cover data. Processing service encapsulation and service composition methods were also developed for chaining various land cover services to a complex service chain. Finally, a prototype system was implemented to evaluate the validity and feasibility of the proposed DSCM. Two walk-through examples were performed with GlobeLand30 datasets and muti-temporal Landsat imagery, respectively. The experimental results indicate that the proposed DSCM approach was more effective and applicable to a wider range of issues in land cover change detection.