bluuebunny/crossref_metadata_2025 huggingface dataset:
Dysregulation of CD4+CD25high T Cells in the Synovial Fluid of Patients With Antibiotic‐Refractory Lyme Arthritis
Authors: Vudattu Nalini K. , Strle Klemen , Steere Allen C. , Drouin Elise E.
Published: March 2013

Objective

To examine the role of immune dysregulation in antibiotic‐refractory Lyme arthritis by comparing the phenotype, frequency, and function of CD4+ Teff cells and Treg cells in patients with antibiotic‐ responsive arthritis and patients with antibiotic‐refractory arthritis.

Methods

Matched peripheral blood and synovial fluid samples from 15 patients with antibiotic‐responsive arthritis were compared with those from 16 patients with antibiotic‐refractory arthritis, using flow cytometry, suppression assays, and cytokine assays.

Results

Critical differences between the 2 patient groups were observed in the synovial fluid CD4+CD25high population, a cell subset usually composed of FoxP3‐positive Treg cells. In patients with antibiotic‐refractory arthritis, this cell population often had fewer FoxP3‐positive cells and a greater frequency of FoxP3‐negative (Teff) cells compared with patients with antibiotic‐responsive arthritis. Moreover, the expression of glucocorticoid‐induced tumor necrosis factor receptor and OX40 on CD4+CD25high cells was significantly higher in the antibiotic‐refractory group. Suppression assays showed that CD4+CD25high cells in patients with antibiotic‐refractory arthritis did not effectively suppress proliferation of CD4+CD25− cells or secretion of interferon‐γ and tumor necrosis factor α, whereas those cells in patients with antibiotic‐responsive arthritis did suppress proliferation of CD4+CD25− cells and secretion of interferon‐γ and tumor necrosis factor α. Finally, in the antibiotic‐refractory group, higher ratios of CD25high FoxP3‐negative cells to CD25high FoxP3‐positive cells correlated directly with a longer duration of arthritis after antibiotic treatment.

Conclusion

Patients with antibiotic‐refractory Lyme arthritis often have lower frequencies of Treg cells, higher expression of activation coreceptors, and less effective inhibition of proinflammatory cytokines. This suggests that immune responses in these patients are excessively amplified, leading to immune dysregulation and antibiotic‐refractory arthritis.
Anti–Citrullinated Protein Antibodies in Unaffected First‐Degree Relatives of Rheumatoid Arthritis Patients
Authors: Barra Lillian , Scinocca Mathias , Saunders Sheri , Bhayana Rajesh , Rohekar Sherry , Racapé Maud , Coles Robert , Cairns Ewa , Bell David A.
Published: March 2013

Objective

First‐degree relatives (FDRs) of rheumatoid arthritis (RA) patients sharing genetic and environmental risk factors for RA may represent a pre‐RA state. Since anti–cyclic citrullinated protein/peptide antibodies (ACPAs) appear years before the onset of RA, the purpose of this study was to determine the prevalence of various ACPAs in FDRs of RA patients.

Methods

We evaluated 88 RA patients, 50 unaffected FDRs, and 20 healthy control subjects. Six different types of ACPAs were determined by enzyme‐linked immunosorbent assay. Joint and periodontal disease symptoms were self‐reported. Patients and FDRs were HLA typed for the shared epitope (SE) and the RA‐protective alleles HLA–DRB*1301/1302.

Results

FDRs had a high prevalence of ACPAs (48%) as compared to controls (10%). Prevalence of the SE and smoking in FDRs was also high (62% and 49%, respectively). Of all of the ACPAs in the FDRs, 13 of 32 (41%) were of the IgA isotype. The most commonly expressed IgG ACPA targeted citrullinated vimentin, occurring in 20% of FDRs. The FDRs had an average of 1 type of ACPA, whereas the RA patients expressed a median of 5 different ACPAs. The only FDR to later develop RA expressed 4 different ACPAs. Joint and periodontal disease symptoms in the FDRs were significantly associated with smoking (OR 5.714 [95% confidence interval (95% CI) 1.151–28.3] and OR 12.25 [95% CI 2.544–58.99], respectively), but not with ACPAs.

Conclusion

The rate of ACPA positivity in unaffected FDRs of RA patients with a high prevalence of the SE and smoking was 48%, whereas ACPAs were rare in the healthy controls. ACPAs in the FDRs of RA patients was most commonly of the IgA isotype, but IgG ACPA targeting citrullinated vimentin was also frequently found.
Toll‐like Receptor 2 Is Required for Autoantibody Production and Development of Renal Disease in Pristane‐Induced Lupus
Authors: Urbonaviciute Vilma , Starke Charlotte , Pirschel Wiebke , Pohle Sandy , Frey Silke , Daniel Christoph , Amann Kerstin , Schett Georg , Herrmann Martin , Voll Reinhard E.
Published: March 2013

Objective

The mechanisms involved in breaking immunologic tolerance against nuclear autoantigens in systemic lupus erythematosus (SLE) are not fully understood. Our recent studies in nonautoimmune mice provided evidence of an important role of Toll‐like receptor 2 (TLR‐2) in antichromatin autoantibody induction by high mobility group box chromosomal protein 1–nucleosome complexes derived from apoptotic cells. The objective of this study was to investigate whether TLR‐2 signaling is required for the induction of autoantibodies and the development of SLE‐like disease in murine pristane‐induced lupus.

Methods

Lupus‐like disease in C57BL/6 and TLR‐2−/− mice was induced by pristane injection. The numbers of immune cells and serum cytokine concentrations were determined by flow cytometry. Renal disease was assessed by quantification of proteinuria, histologic analyses, and enzyme‐linked immunospot assay.

Results

Pristane‐injected TLR‐2−/− mice generated reduced numbers of splenic CD138+/cytoplasmic κL/λL chain–positive plasma cells and displayed diminished IgG responses against double‐stranded DNA, histones, nucleosomes, some extractable nuclear autoantigens, and cardiolipin when compared with wild‐ type controls. TLR‐2 deficiency prevented the pristane‐induced systemic release of interleukin‐6 (IL‐6) and IL‐10. The absence of TLR‐2 attenuated peritoneal recruitment of CD11c+ cells and formation of lipogranulomas. Importantly, the renal disease that developed in pristane‐treated TLR‐2−/− mice was less severe than that in control mice, as reflected by milder proteinuria, reduced glomerular deposition of IgG and complement, and decreased renal infiltration of autoantibody‐secreting cells.

Conclusion

TLR‐2 is required for the production of prototypical lupus autoantibodies and the development of renal disease in pristane‐induced murine lupus. Interference with TLR‐2 signaling may be a promising novel strategy for the treatment of SLE.
In vitro metabolism of obovatol and its effect on cytochrome P450 enzyme activities in human liver microsomes
Authors: Joo Jeongmin , Lee Doohyun , Wu Zhexue , Shin Jung‐Hoon , Lee Hye Suk , Kwon Byoung‐Mog , Huh Tae‐Lin , Kim Yang‐Weon , Lee Su‐Jun , Kim Tae Wan , Lee Taeho , Liu Kwang‐Hyeon
Published: March 2013

Obovatol, a major constituent of the leaves of Magnolia obovata Thunb, is known to inhibit nuclear factor‐κB activity and arachidonic acid‐induced platelet aggregation. This study was performed to identify the metabolites of obovatol in human liver microsomes. Human liver microsomes incubated with obovatol in the presence of NADPH and/or UDPGA resulted in the formation of six metabolites, M1–M6. M1 and M2 were identified as hydroxyobovatol, on the basis of liquid chromatography/tandem mass spectrometric (LC‐MS/MS) analysis. M1, M2 and obovatol were further metabolized to their glucuronide conjugates, obovatol‐glucuronide (M3), obovatol‐diglucuronide (M4) and hydroxyobovatol‐glucuronide (M5 and M6). The inhibitory potency of obovatol on eight major human P450s was also investigated in human liver microsomes. In these experiments, obovatol strongly inhibited CYP2C19‐mediated S‐mephenytoin hydroxylase activity with an IC50 value of 0.8 µm, which could have implications for drug–drug interactions. Copyright © 2013 John Wiley & Sons, Ltd.
The influence of misclassification bias on the reported rates of congenital anomalies on the birth certificates for West Virginia—A consequence of an open‐ended query
Authors: Li Ji , Robbins Shayhan , Lamm Steven H.
Published: March 2013

BACKGROUND

Passive surveillance for congenital anomalies using birth certificates are generally considered to have biased reporting, though the sources of those biases are not well‐known nor controlled for. We have analyzed the congenital anomaly reporting data for 418,385 live births in West Virginia (1990–2009) from the 1989 US standard birth certificate and have newly identified a particular source of bias.

METHODS

Congenital anomaly prevalence rates per 100 live births have been determined for both specified birth defects and for other congenital anomalies by county, by hospital, and by year. Extreme outliers were identified by z score. Text strings for “other congenital anomaly” reports recorded for 1998–2009 were assessed for information on congenital anomalies.

RESULTS

While rates for specified birth defects reported in checked‐box format showed little variation, rates for “other congenital anomaly” collected in open‐ended format showed much variation. Nearly half of the “other congenital anomaly” reports were for neonatal conditions rather than for major structural congenital anomalies. This misclassification alone had elevated the state‐wide congenital anomaly reporting rate from 1.1 to 1.8% of live births. Geographic clustering and a temporal bulge in congenital anomaly reports disappeared after misclassified data were removed.

CONCLUSIONS

Data collected in checked‐box format on specified birth defects showed consistent patterns over time and space, while data collected in open‐ended format on “other congenital anomalies” showed an epidemiological pattern reflecting neonatal conditions rather than birth defects. The 2003 US standard birth certificate wisely limits data collection to specified birth defects using the checked‐box format. Birth Defects Research (Part A), 2013. © 2013 Wiley Periodicals, Inc.
Systems metabolic engineering of xylose‐utilizing Corynebacterium glutamicum for production of 1,5‐diaminopentane
Authors: Buschke Nele , Becker Judith , Schäfer Rudolf , Kiefer Patrick , Biedendieck Rebekka , Wittmann Christoph
Published: March 2013

The sustainable production of industrial platform chemicals is one of the great challenges facing the biotechnology field. Ideally, fermentation feedstocks would rather rely on industrial waste streams than on food‐based raw materials. Corynebacterium glutamicum was metabolically engineered to produce the bio‐nylon precursor 1,5‐diaminopentane from the hemicellulose sugar xylose. Comparison of a basic diaminopentane producer strain on xylose and glucose feedstocks revealed a 30% reduction in diaminopentane yield and productivity on the pentose sugar. The integration of in vivo and in silico metabolic flux analysis by 13C and elementary modes identified bottlenecks in the pentose phosphate pathway and the tricarboxylic acid cycle that limited performance on xylose. By the integration of global transcriptome profiling, this could be specifically targeted to the tkt operon, genes that encode for fructose bisphosphatase (fbp) and isocitrate dehydrogenase (icd), and to genes involved in formation of lysine (lysE) and N‐acetyl diaminopentane (act). This was used to create the C. glutamicum strain DAP‐Xyl1 icdGTG Peftufbp Psodtkt Δact ΔlysE. The novel producer, designated DAP‐Xyl2, exhibited a 54% increase in product yield to 233 mmol mol–1 and a 100% increase in productivity to 1 mmol g–1 h–1 on the xylose substrate. In a fed‐batch process, the strain achieved 103 g L–1 of diaminopentane from xylose with a product yield of 32%. Xylose utilization is currently one of the most relevant metabolic engineering subjects. In this regard, the current work is a milestone in industrial strain engineering of C. glutamicum.

See accompanying commentary by Hiroshi Shimizu DOI: 10.1002/biot.201300097
Slug increases sensitivity to tubulin‐binding agents via the downregulation of βIII and βIVa‐tubulin in lung cancer cells
Authors: Tamura Daisuke , Arao Tokuzo , Nagai Tomoyuki , Kaneda Hiroyasu , Aomatsu Keiichi , Fujita Yoshihiko , Matsumoto Kazuko , De Velasco Marco A. , Kato Hiroaki , Hayashi Hidetoshi , Yoshida Shuhei , Kimura Hideharu , Maniwa Yoshimasa , Nishio Wataru , Sakai Yasuhiro , Ohbayashi Chiho , Kotani Yoshikazu , Nishimura Yoshihiro , Nishio Kazuto
Published: March 2013

Transcription factor Slug/SNAI2 (snail homolog 2) plays a key role in the induction of the epithelial mesenchymal transition in cancer cells; however, whether the overexpression of Slug mediates the malignant phenotype and alters drug sensitivity in lung cancer cells remains largely unclear. We investigated Slug focusing on its biological function and involvement in drug sensitivity in lung cancer cells. Stable Slug transfectants showed typical morphological changes compared with control cells. Slug overexpression did not change the cellular proliferations; however, migration activity and anchorage‐independent growth activity with an antiapoptotic effect were increased. Interestingly, stable Slug overexpression increased drug sensitivity to tubulin‐binding agents including vinorelbine, vincristine, and paclitaxel (5.8‐ to 8.9‐fold increase) in several lung cancer cell lines but did not increase sensitivity to agents other than tubulin‐binding agents. Real‐time RT‐PCR (polymerase chain reaction) and western blotting revealed that Slug overexpression downregulated the expression of βIII and βIVa‐tubulin, which is considered to be a major factor determining sensitivity to tubulin‐binding agents. A luciferase reporter assay confirmed that Slug suppressed the promoter activity of βIVa‐tubulin at a transcriptional level. Slug overexpression enhanced tumor growth, whereas Slug overexpression increased drug sensitivity to vinorelbine with the downregulation of βIII and βIV‐tubulin in vivo. Immunohistochemistry of Slug with clinical lung cancer samples showed that Slug overexpression tended to be involved in response to tubulin‐binding agents. In conclusion, our data indicate that Slug mediates an aggressive phenotype including enhanced migration activity, anoikis suppression, and tumor growth, but increases sensitivity to tubulin‐binding agents via the downregulation of βIII and βIVa‐tubulin in lung cancer cells.
Lessons on Child Protection: A Survey of Newly Qualified Primary‐Level Teachers in Ireland
Authors: McGarry Kathryn , Buckley Helen
Published: March 2013

Do all newly qualified primary‐level teachers report the same experience of child protection practices in their schools? Drawing on findings from a recent Irish study, this paper will consider the operation of official child protection guidelines in schools based on the reported awareness and knowledge of a sample of newly qualified teachers. Their knowledge of child protection guidelines pertains to experience of induction into child protection practices upon taking up employment in the school and their awareness of a designated liaison person, school child protection policy and the procedures for reporting a suspected child protection issue within the school. The findings of this research indicate that schools' compliance with national guidelines on child protection is certainly inconsistent and such anad hocapproach can be seen to impact on the perceived confidence levels of newly qualified teachers in dealing with child protection issues in the school. Given the crucial role teachers play in the safeguarding of children and the potential for strengthening this role, this paper argues for the standardisation of the operation of child protection procedures across all schools and the provision of training for all school staff. Copyright © 2013 John Wiley & Sons, Ltd.

‘This paper argues for the standardisation of the operation of child protection procedures’

Key Practitioner Message

There is a clear need for enhanced, standardised pre‐service and in‐school child protection training.

New entrants to the teaching profession should automatically be inducted into the school child protection policy.

Schools should have clearly articulated child protection policies and action plans which are consistently communicated to staff on a regular basis.

The potential for teachers to promote child welfare and protection should be acknowledged and realised.
Conduction disorders in the setting of transcatheter aortic valve implantation: a clinical perspective
Authors: Fraccaro Chiara , Napodano Massimo , Tarantini Giuseppe
Published: March 2013

The presence of periprocedural conduction disorders (CDs) and the need for permanent pacemaker (PPM) after transcatheter aortic valve implantation (TAVI) are frequent findings in clinical practice. Notwithstanding, robust information on the prognostic and therapeutic implications of these complications are lacking. The newly occurrence of CD after TAVI seems related to the trauma of the conduction system during procedure. On the contrary, major predictors for PPM implantation after TAVI seem to be the use of CoreValve prosthesis (Medtronic, Minneapolis, MN) and the presence of CD before TAVI. An accurate pre‐TAVI screening, careful valve implantation, as well as post‐TAVI monitoring must be pursued to prevent avoidable PPM implantation. The aim of this report is to analyze the available data on this field and to propose some practical clinical tips to prevent or to manage these complications. © 2013 Wiley Periodicals, Inc.
Self‐Organization of Honeycomb‐like Porous TiO2 Films by means of the Breath‐Figure Method for Surface Modification of Titanium Implants
Authors: Li Hong , Jia Yi , Du Mingchun , Fei Jinbo , Zhao Jie , Cui Yue , Li Junbai
Published: March 2013

This study describes a facile breath‐figure method for the preparation of honeycomb‐like porous TiO2 films with an organometallic small‐molecule precursor. Multiple characterization techniques have been used to investigate the porous films and a mechanism for the formation process of porous TiO2 films through the breath‐figure method is proposed. The pore size of the TiO2 films could be modulated by varying the experimental parameters, such as the concentration of titanium n‐butoxide (TBT) solution, the content of cosolvent, and the air flow rate. In vitro cell‐culture experiments indicate that NIH 3T3 fibroblast cells seeded on the honeycomb‐like porous TiO2 films show good adhesion, spreading, and proliferation behaviors, which suggests that honeycomb‐like porous TiO2 films are an attractive biomaterial for surface modification of titanium and its alloys implants in tissue engineering to enhance their biocompatibility and bioactivity.
One‐Pot Synthesis of Pyrazoles through a Four‐Step Cascade Sequence
Authors: Hao Lu , Hong Jun‐Jie , Zhu Jun , Zhan Zhuang‐Ping
Published: March 2013

A one‐pot synthesis of 3,4,5‐ and 1,3,5‐pyrazoles from tertiary propargylic alcohols and para‐tolylsulfonohydrazide has been accomplished. The pyrazoles are formed through a four‐step cascade sequence, including FeCl3‐catalyzed propargylic substitution, aza‐Meyer–Schuster rearrangement, base‐mediated 6π electrocyclization, and thermal [1,5] sigmatropic shift. In this reaction, the 3,4,5‐ and 1,3,5‐pyrazoles are produced selectively according to different substituents in the starting alcohols.
ChemInform Abstract: Structure of Li4B2O5: High‐Temperature Monoclinic and Low‐Temperature Orthorhombic Forms.
Authors: He Meng , Okudera Hiroki , Simon Arndt , Koehler J. , Jin Shifeng , Chen Xiaolong
Published: March 2013

The structures of two Li4B2O5 polymorphs are determined by single crystal XRD and DFT calculations.
ChemInform Abstract: Sensitized Red Luminescence from Mn2+‐Doped Olgite‐Type Phosphates.
Authors: Yonesaki Yoshinori
Published: March 2013

The parity‐ and spin‐forbidden weak red emission of Na2BaMgP2O8:Mn2+ is successfully sensitized by Eu2+ or Ce3+ codoping, so that Eu2+ or Ce3+ codoped Na2BaMgP2O8:Mn2+ exhibits bright red luminescence upon UV excitation.
ChemInform Abstract: Crystal Structure, Morphotropic Phase Transition and Luminescence in the New Cyclosilicates Sr3Ln2(Si3O9)2, Ln: Y, Eu—Lu.
Authors: Tyutyunnik Alexander P. , Leonidov Ivan I. , Surat Ludmila L. , Berger Ivan F. , Zubkov Vladimir G.
Published: March 2013

The title compounds are synthesized by solid state reaction of stoichiometric mixtures of SrCO3, Ln2O3 (Ln: Y, Eu—Lu), and amorphous SiO2 (air, alumina boats, 1300 °C, 150—200 h).
ChemInform Abstract: High Temperature Synthesis of Two Open‐Framework Uranyl Silicates with Ten‐Ring Channels: Cs2(UO2)2Si8O19 and Rb2(UO2)2Si5O13.
Authors: Babo Jean‐Marie , Albrecht‐Schmitt Thomas E.
Published: March 2013

The new title compounds are synthesized from stoichiometric mixtures of U, SiO2, TeO2, and excess MCl (M: Rb, Cs) as reactive flux (evacuated silica tubes, 900 °C, 5 d).
ChemInform Abstract: Unusual Coordination for Plutonium(IV), Cerium(IV), and Zirconium(IV) in the Cationic Layered Materials [M2Te4O11]X2 (M: Pu, Ce, Zr; X: Cl, Br).
Authors: Lin Jian , Cross Justin N. , Diwu Juan , Polinski Matthew J. , Villa Eric M. , Albrecht‐Schmitt Thomas E.
Published: March 2013

Compounds (III) and (V) are isotypic and crystallize in the triclinic space group P$\bar 1$ with Z = 2 (single crystal XRD).
ChemInform Abstract: Cross‐trienamines in Asymmetric Organocatalysis.
Authors: Halskov Kim Soeholm , Johansen Tore Kiilerich , Davis Rebecca L. , Steurer Marianne , Jensen Frank , Joergensen Karl Anker
Published: March 2013

Cross‐conjugated trienamines are introduced as a new concept in asymmetric organocatalysis.
ChemInform Abstract: Preparation and Characterization of New C2‐ and C1‐Symmetric Nitrogen, Oxygen, Phosphorous, and Sulfur Derivatives and Analogues of TADDOL. Part 1. Compounds Containing One or Two Sulfur‐Containing Substituents and Use in Cu‐Catalyzed Enantioselective Conjugate Additions to Cyclic Enones.
Authors: Pichota Arkadius , Gramlich Volker , Beck Albert K. , Seebach Dieter
Published: March 2013

The crystal structures of 16 representatives of the title TADDOL analogues are reported.
ChemInform Abstract: N‐2‐Iodobenzylcinchoninium Bromide is Effective for Catalytic Enantioselective Trifluoromethylation of Azomethine Imines in Solkane® 365mfc.
Authors: Okusu Satoshi , Kawai Hiroyuki , Xu Xiu‐Hua , Tokunaga Etsuko , Shibata Norio
Published: March 2013

Solkane® 365mfc is proven to be an environmentally benign alternative solvent for the catalytic enantioselective trifluoromethylation of azomethine imines (I).
ChemInform Abstract: Chalcogenoalkynes: Precursors for the Regioselective Preparation of 2‐Chalcogeno‐1‐halonaphthalenes Through [4 + 2] Cycloaddition.
Authors: Mantovani Anderson C. , Back Davi F. , Zeni Gilson
Published: March 2013

The present cycloaddition of alkynyl chalcogenides (II) with various o‐alkynylbenzaldehydes is promoted by CuCl2 to furnish the corresponding chalcogeno‐1‐halonaphthalenes in good yields.
ChemInform Abstract: First Intramolecular Alkylation of Nitriles with Primary and Secondary Alcohols Catalyzed by Iridium Complexes.
Authors: Anxionnat Bruno , Gomez Pardo Domingo , Ricci Gino , Cossy Janine
Published: March 2013

The first iridium‐catalyzed intramolecular alkylation of hydroxynitriles is described.
ChemInform Abstract: Replacement of BF4‐ by PF6‐ Makes Selectfluor Greener.
Authors: Jin Zhuang , Xu Bo , DiMagno Stephen G. , Hammond Gerald B.
Published: March 2013

Changing the counterion in Selectfluor® from tetrafluoroborate to hexafluorophosphate significantly reduces catalyst loading in amide oxidation reactions.
ChemInform Abstract: Forming Tertiary Organolithiums and Organocuprates from Nitrile Precursors and Their Bimolecular Reactions with Carbon Electrophiles to Form Quaternary Carbon Stereocenters.
Authors: Schnermann Martin J. , Untiedt Nicholas L. , Jimenez‐Oses Gonzalo , Houk Kendall N. , Overman Larry E.
Published: March 2013

A general procedure for the synthesis of the organo‐metal reagents and their highly diastereoselective reaction with carbon electrophiles from the sterically more hindered concave face is presented.
ChemInform Abstract: Biomimetic Approach for the Synthesis of N,N′‐Diarylsubstituted Formamidines Catalyzed by β‐Cyclodextrin in Water.
Authors: Patil Dipak R. , Dalal Dipak S.
Published: March 2013

A facile procedure employing β‐cyclodextrin as reusable catalyst allows the synthesis of the title compounds (III) via reaction of triethylorthoformate with anilines.
ChemInform Abstract: Study on the Coupling of Acyclic Esters with Alkenes — The Synthesis of 2‐(2‐Hydroxyalkyl)cyclopropanols via Cascade Cyclization Using Allylsamarium Bromide.
Authors: Tu Yawei , Zhou Liejin , Yin Ruifeng , Lv Xin , Flowers Robert A. II , Choquette Kimberly A. , Liu Huili , Niu Qingsheng , Wang Xiaoxia
Published: March 2013

HMPA and water as additives inhibit the nucleophilicity and enhance the SET ability of the Sm agent.
ChemInform Abstract: Direct Allenol‐Based Stereocontrolled Access to Substituted (E)‐1,3‐Enynes.
Authors: Alcaide Benito , Almendros Pedro , Martinez del Campo Teresa
Published: March 2013

The direct approach for the stereoselective synthesis of functionalized enynes by treatment of aryl‐substituted α‐allenols with an aqueous Ac‐Cl/NaOH system possibly proceeds via elimination of acetic acid, made up of a δ‐hydrogen and the acetate group in the initially formed α‐allenic acetate.
ChemInform Abstract: Metal‐Free Direct Amination/Aromatization of 2‐Cyclohexenones to Iodo‐N‐arylanilines and N‐Arylanilines Promoted by Iodine.
Authors: Barros M. Teresa , Dey Suvendu S. , Maycock Christopher D. , Rodrigues Paula
Published: March 2013

Different amounts of iodine are necessary to produce the target compounds.
ChemInform Abstract: Cobalt‐Catalyzed Alkyne—Dihalomethane—Amine Coupling: An Efficient Route for Propargylamines.
Authors: Tang Yanchi , Xiao Tiebo , Zhou Lei
Published: March 2013

The transformation can be made with the cheap CoBr2 in the absence of phosphine ligands and various functionalities are tolerated.
ChemInform Abstract: One‐Pot Sequential Synthesis of Ethers from an Aliphatic Carboxylic Acid and an Alcohol by Indium‐Catalyzed Deoxygenation of an Ester.
Authors: Sakai Norio , Usui Yuta , Moriya Toshimitsu , Ikeda Reiko , Konakahara Takeo
Published: March 2013

A wide range of ethers is synthesized by the present new method tolerating several functionalities including alkenes, halogens, nitro and heterocyclic groups.
ChemInform Abstract: Decarboxylative Allylation of Glyoxylic Acids with Diallyl Carbonate.
Authors: Manjolinho Filipe , Gruenberg Matthias F. , Rodriguez Nuria , Goossen Lukas J.
Published: March 2013

The new method is based on the use of a Pd(0)/phospine catalyst and diallyl carbonate as the allyl source.
ChemInform Abstract: Palladium‐Catalyzed α‐Regioselective Allylic Amination of Morita—Baylis—Hillman Acetates with Simple Amines.
Authors: Wang Yan , Liu Li , Wang Dong , Chen Yong‐Jun
Published: March 2013

The excellent regioselectivity is controlled by the Pd‐catalyst/ligand system and depends on the substrate used.
ChemInform Abstract: Oxidative Nucleophilic Substitution of Hydrogen in Nitro(pentafluorosulfanyl)benzenes with Alkyl Grignard and Lithium Reagents.
Authors: Vida Norbert , Beier Petr
Published: March 2013

Alkyl Grignard and lithium reagents undergo nucleophilic addition to para‐ and meta‐nitro(pentafluorosulfanyl)benzenes (I) and (IV).
ChemInform Abstract: Silver(I)‐Mediated Reaction of Trimethylsilylated Arylacetylenes with Sulfonyl Chlorides: Unexpected Formation of Vinyl Sulfones.
Authors: Deng Gui Sheng , Sun Teng Fei
Published: March 2013

Silver nitrate mediated reactions of alkynylsilanes with sulfonyl chlorides provide E/Z‐mixtures of vinyl silanes instead of expected alkynyl sulfones.
ChemInform Abstract: Microwave‐Mediated Suzuki—Miyaura Cross‐Couplings of Thioether‐ and ortho‐Substituted Methylphenylboronic Acid Esters.
Authors: Baltus Christine B. , Press Neil J. , Spencer John
Published: March 2013

Suitable conditions A) or B) are presented for the hitherto unsuccessful process.
ChemInform Abstract: Ferrous Salt Promoted Homocoupling of Arenediazonium Tetrafluoroborates under Mild Conditions.
Authors: Ding Yiyuan , Cheng Kai , Qi Chenze , Song Qingbao
Published: March 2013

The method allows a simple and efficient preparation of symmetrical biaryls.
ChemInform Abstract: Facile Coupling of Propargylic, Allylic and Benzylic Alcohols with Allylsilane and Alkynylsilane, and Their Deoxygenation with Et3SiH, Catalyzed by Bi(OTf)3 in [BMIM][BF4] Ionic Liquid (IL), with Recycling and Reuse of the IL.
Authors: Kumar G. G. K. S. Narayana , Laali Kenneth K.
Published: March 2013

A Lewis acid and an ionic liquid as the solvent provide a well‐working catalytic system for allylation, deoxygenation and alkynylation of propargylic, allylic and benzylic alcohols.
ChemInform Abstract: Convenient Synthesis of 1,4‐Dihydroxy‐2‐(ω‐hydroxyalkoxy)anthracene‐9,10‐diones and Their Conjugation with D‐Glucal.
Authors: Budniok Sebastian , Walczak Krzysztof Z.
Published: March 2013

The alkylation of trihydroxy substrates (I) with ω‐bromo alcohols (II) proceeds regioselectively at the 2‐position.
ChemInform Abstract: Synthesis of 2,5‐Substituted Tetrahydrofurans by Pd(II)‐Catalyzed Cyclization.
Authors: Olszewska Beata , Kryczka Boguslaw , Zawisza Anna
Published: March 2013

The title reaction of alcohols (IIb)‐(IIe) produces diastereomeric mixtures of the furan derivatives.
ChemInform Abstract: Facile Synthesis of 2‐Iodo‐spiro[indene‐1,1′‐isobenzofuran]‐3′‐ones via Iodine‐Promoted Cascade Cyclization.
Authors: Zhu Hai‐Tao , Dong Xue , Wang Li‐Jing , Zhong Mei‐Jin , Liu Xue‐Yuan , Liang Yong‐Min
Published: March 2013

A novel method is elaborated for the synthesis of spiro derivatives (II) by iodine‐mediated cyclization of benzoates (I).
ChemInform Abstract: Novel Methods for the Synthesis of 2‐[(4R)‐2‐Oxo‐4‐phenylpyrrolidin‐1‐yl]acetamide ((R)‐Phenotropil).
Authors: Vorona M. , Veinberg G. , Vikainis S. , Kuznetsov E. , Lebedev A. , Ponomarev Yu. , Chernobrovijs A. , Zvejniece L. , Dambrova M.
Published: March 2013

Two novel routes for the synthesis of (R)‐phenotropil (IV) are developed starting from (3R)‐4‐amino‐3‐phenylbutyrate (I).
ChemInform Abstract: Palladium‐Catalyzed Atom Transfer Radical Cyclization of Unactivated Alkyl Iodide.
Authors: Liu Hui , Qiao Zongjun , Jiang Xuefeng
Published: March 2013

A palladium‐catalyzed iodide atom transfer cyclization is achieved with a wide range of 5‐hexenyl iodides, delivering alkyl iodide derivatives in moderate to good yields.
ChemInform Abstract: Triphenylphosphine‐Mediated Reaction of Dialkyl Azodicarboxylate with Activated Alkenes Leading to Pyrazolines.
Authors: Yamazaki Shoko , Maenaka Yuta , Fujinami Ken , Mikata Yuji
Published: March 2013

Huisgen zwitterions, derived from triphenylphosphine and azodicarboxylates undergo a cycloaddition reaction with activated alkenes to give the pyrazolines.
ChemInform Abstract: Palladium‐Catalyzed Desulfitative Arylation of Azoles with Arylsulfonyl Hydrazides.
Authors: Yu Xinzhang , Li Xingwei , Wan Boshun
Published: March 2013

A palladium‐catalyzed oxidative coupling reaction of azoles with arylsulfonyl hydrazides affords the desired products in moderate to high yields.
ChemInform Abstract: [3 + 2] Cycloaddition Reactions of Diethyl (E)‐2‐Fluoromaleate.
Authors: Patrick Timothy B. , Shadmehr Mehrdad , Khan Akbar H. , Singh Rajiv K. , Asmelash Bethel
Published: March 2013

Diethyl (E)‐2‐fluoromaleate (II), easily accessible via a Horner—Wadsworth—Emmons Wittig procedure, undergoes [3 + 2] cycloadditions with aromatic α‐iminoesters (I) and aromatic nitrones (IV) to furnish polysubstituted pyrrole (III) or isoxazole derivatives (V), resp., in excellent regio‐ as well as stereoselectivity.
ChemInform Abstract: Preparation of Cu(O‐Ac)2/MCM‐41 Catalyst and Its Application in the One‐Pot Synthesis of 1,2,3‐Triazoles in Water.
Authors: Hosseinzadeh Rahman , Sepehrian Hamid , Shahrokhi Farshid
Published: March 2013

The three‐component coupling between bromides, terminal alkynes and sodium azide forms the required triazoles in a regioselective manner.
ChemInform Abstract: A Diversified Assembly of 1,2,4‐Oxadiazol‐3‐amines: Metallic Thiophile Catalyzed Chemoselective One‐Pot Reaction of Aryl Isothiocyanates, Amidines/Guanidines, and Hydroxylamine.
Authors: Jalani Hitesh B. , Sudarsanam V. , Vasu Kamala K.
Published: March 2013

An efficient two‐step three‐component reaction of isothiocyanates, iminocarboxylic acids and a guanidine or hydroxylamine is developed to give 1,2,4‐oxadiazoles in a one‐pot reaction.
ChemInform Abstract: Solvent‐Free L‐Proline‐Catalyzed Domino Knoevenagel/6π‐Electrocyclization for the Synthesis of Highly Functionalized 2H‐Pyrans.
Authors: Pena Javier , Moro Rosalina F. , Basabe P. , Marcos Isidro S. , Diez David
Published: March 2013

The pyrans are prepared from 3,3‐dialkyl‐substituted α,β‐unsaturated aldehydes, including natural products, and β‐oxosulfones.
ChemInform Abstract: Functionalization of Chromones Through an Aza‐Baylis—Hillman‐Type Reaction.
Authors: Terzidis Michael A. , Tsiaras Vassilios G. , Stephanidou‐Stephanidou Julia , Tsoleridis Constantinos A. , Psycharis Vassilis , Raptopoulou Catherine P.
Published: March 2013

Application of the aza‐Baylis—Hillman reaction of 3‐formylchromones with dialkyl azodicarboxylates results in substitution of the formyl group with a hydrazine moiety.
ChemInform Abstract: Concise Approach to 1,4‐Dioxygenated Xanthones via Novel Application of the Moore Rearrangement.
Authors: Nichols Alexander L. , Zhang Patricia , Martin Stephen F.
Published: March 2013

A general route is developed for xanthones (V), which are otherwise difficult to obtain, via Moore rearrangement (II) → (III).
ChemInform Abstract: Aza‐Oxindole Synthesis via Base‐Promoted Truce—Smiles Rearrangement.
Authors: Dey Chandan , Katayev Dmitry , Ylijoki Kai E. O. , Kuendig E. Peter
Published: March 2013

The biologically important heterocyclic system is readily available from substrates of type (I) and (III).
ChemInform Abstract: Lewis Acid Mediated Ring Exchange Reaction of Dihydrobenzofurans and Its Application to the Formal Total Synthesis of (‐)‐Quinocarcinamide (VIII).
Authors: Chiba Hiroaki , Sakai Yuki , Oishi Shinya , Fujii Nobutaka , Ohno Hiroaki
Published: March 2013

The tricyclic system of the substrates is the key structural element for the ring exchange to give oxazolidinones.
ChemInform Abstract: Efficient Synthesis of Novel Tetrahydropyrrolo[3′,4′:3,4]pyrrolo[2,1‐a]isoquinoline Derivatives via a Simple and Convenient MCR in Aqueous Micellar System.
Authors: Mondal Shyamal , Maity Arindam , Paira Rupankar , Banerjee Maitreyee , Bharitkar Yogesh P. , Hazra Abhijit , Banerjee Sukdeb , Mondal Nirup B.
Published: March 2013

A facile synthesis of the title compounds (16 examples) is completed by 1,3‐dipolar cycloaddition of isoquinolinium ylides to phenyl maleimide derivatives.
ChemInform Abstract: Highly Enantioselective Catalytic [6 + 3] Cycloadditions of Azomethine Ylides.
Authors: Potowski Marco , Bauer Jonathan O. , Strohmann Carsten , Antonchick Andrey P. , Waldmann Herbert
Published: March 2013

The highly regio‐ and enantioselective reaction yields complex annulated piperidines with 8 stereocenters.
ChemInform Abstract: 22% Co/CeO2‐ZrO2‐Catalyzed Synthesis of 1,2,3,4‐Tetrahydro‐2‐pyrimidinones and ‐thiones.
Authors: Biklarian Hajar , Behbahani Farahnaz K. , Fakhroueian Zahra
Published: March 2013

The system Co/CeO2‐ZrO2 is found to be an efficient, and given catalyst for the synthesis of 1,2,3,4‐tetrahydro‐2‐pyrimidinones and ‐thiones.
ChemInform Abstract: AlCl3‐Induced C—N Bond Formation Followed by Pd/C—Cu‐Mediated Coupling—Cyclization Strategy: Synthesis of Pyrrolo[2,3‐b]quinoxalines as Anticancer Agents.
Authors: Prasad Bagineni , Kumar K. Shiva , Babu P. Vijaya , Anusha K. , Rambabu D. , Kandale Ajit , Vanaja G. R. , Kalle Arunasree M. , Pal Manojit
Published: March 2013

The monosubstituted products (III) are readily converted to the target structures in the presence of terminal alkynes.
ChemInform Abstract: Micelle Promoted Supramolecular Carbohydrate Scaffold‐Catalyzed Multicomponent Synthesis of 1,2‐Dihydro‐1‐aryl‐3H‐naphth[1,2‐e][1,3]oxazin‐3‐one and Amidoalkyl Naphthols Derivatives in Aqueous Medium.
Authors: Kumar Atul , Gupta Maneesh Kumar , Kumar Mukesh
Published: March 2013

An eco‐friendly protocol produces the title compounds in a one‐pot manner, where ring closure takes place via ammonia elimination.
ChemInform Abstract: CuCl—K2CO3‐Catalyzed Highly Selective Borylcupration of Internal Alkynes —Ligand Effect.
Authors: Yuan Weiming , Ma Shengming
Published: March 2013

The copper‐catalyzed borylcupration of internal alkynes with bis(pinacolato)diboron in the presence of potassium carbonate as a base produces (Z)‐alkenylboron compounds regio‐ and stereoselectively.
ChemInform Abstract: Synthesis of Novel 2′‐Spirocyclopropyl‐5′‐deoxyphosphonic Acid Furanosyl Nucleoside Analogues as Potent Antiviral Agents.
Authors: Shen Guang Huan , Hong Joon Hee
Published: March 2013

Novel 5′‐deoxyfuranosyl purine phosphone acid analogues having a 2′‐electropositive cyclopropanoid moiety are designed, synthesized and evaluated for their anti‐HIV‐1 activity.
ChemInform Abstract: Magnetic and Gold‐Coated Magnetic Iron Oxide Nanoparticles as Detection Tools: Preparation, Characterization, and Biosensing Applications
Authors: Kouassi Gilles K.
Published: March 2013

Review: 109 refs.
ChemInform Abstract: Band Engineering of Thermoelectric Materials
Authors: Pei Yanzhong , Wang Heng , Snyder G. J.
Published: March 2013

Review: emphasis on lead chalcogenides; 114 refs
ChemInform Abstract: The Oxide—Water Interface: How Valid Is the Site Dissociation—Surface Equilibria Model?
Authors: Healy Thomas W. , Scales Peter J.
Published: March 2013

Review: 15 refs.
ChemInform Abstract: Short Review on the Nanoarchitectured Pnictides Electrodes for Li Ion Battery
Authors: Monconduit L.
Published: March 2013

Review: 19 refs.
ChemInform Abstract: Zinc Oxide Nanostructures: from Growth to Application
Authors: Gomez Jorge L. , Tigli Onur
Published: March 2013

Review: 72 refs.
ChemInform Abstract: Preparation of Multicomponent Oxides by Mechanochemical Methods
Authors: Fuentes A. F. , Takacs L.
Published: March 2013

Review: 117 refs.
ChemInform Abstract: DNA Metalating—Intercalating Hybrid Agents for the Treatment of Chemoresistant Cancers
Authors: Suryadi Jimmy , Bierbach Ulrich
Published: March 2013

Review: 46 refs.
ChemInform Abstract: Aspects of the Chemistry of Species with Carbon—Polonium Bonds
Authors: Ponikvar‐Svet Maja
Published: March 2013

Review: 92 refs.
ChemInform Abstract: Organochalcogen Ligands and Their Palladium(II) Complexes. Synthesis to Catalytic Activity for Heck Coupling
Authors: Kumar Arun , Kumar Rao Gyandshwar , K Singh Ajai
Published: March 2013

Review: 194 refs.
ChemInform Abstract: Recent Advances in Asymmetric Counteranion‐Directed Catalysis (ACDC).
Authors: Avila Eloah P. , Amarante Giovanni W.
Published: March 2013

Review: 45 refs.
ChemInform Abstract: Low Melting Mixtures in Organic Synthesis — An Alternative to Ionic Liquids?
Authors: Russ Carolin , Koenig Burkhard
Published: March 2013

Review: 96 refs.
ChemInform Abstract: Asymmetric Catalysis Based on tropos Ligands
Authors: Aikawa Kohsuke , Mikami Koichi
Published: March 2013

Review: 172 refs.
ChemInform Abstract: Tertiary Amine and Phosphine Catalyzed Reactions of Ketenes and α‐Halo Ketones
Authors: Chen S. , Salo E. C. , Kerrigan N. J.
Published: March 2013

Review: 76 refs.
ChemInform Abstract: Applications of Aminocatalysis in Target‐Oriented Synthesis
Authors: Christmann M.
Published: March 2013

Review: 31 refs.
ChemInform Abstract: A Significance of Microwave‐Assisted Technique in Green Chemistry
Authors: Bukhariya V. , Shukla A. , Mehta J. , Charde R.
Published: March 2013

Review: 26 refs.
ChemInform Abstract: Acid‐Catalyzed Ring‐Opening Isomerizations of Cyclopropenes
Authors: Phun Lien H. , Aponte‐Guzman Joel , France Stefan
Published: March 2013

Review: 32 refs.
ChemInform Abstract: Domino Twofold Heck/6π‐Electrocyclization Reactions of 1,2‐Dihaloalkenes
Authors: Hussain Munawar , Sung Tran Van , Langer Peter
Published: March 2013

Review: 30 refs.
ChemInform Abstract: 18F Labeling of Arenes
Authors: Tredwell Matthew , Gouverneur Veronique
Published: March 2013

Review: ca. 150 refs.
ChemInform Abstract: A Continuum of Progress: Applications of N‐Heterocyclic Carbene Catalysis in Total Synthesis
Authors: Izquierdo Javier , Hutson Gerri E. , Cohen Daniel T. , Scheidt Karl A.
Published: March 2013

Review: ca. 200 refs.
ChemInform Abstract: Conformational Flexibility and Selectivity in Host—Guest Systems
Authors: Bathori Nikoletta B. , Nassimbeni Luigi R.
Published: March 2013

Review: 27 refs.
ChemInform Abstract: Socioeconomic and Environmental Sustainability Through Recycling of Chemical Wastes
Authors: Oke Oloruntegbe Kunle
Published: March 2013

Review: 18 refs.
ChemInform Abstract: The Analysis of Organic Reaction Pathways by Brownian Processing
Authors: Graham Daniel J.
Published: March 2013

Review: 38 refs.
ChemInform Abstract: Molecular Descriptors and the Electronic Structure
Authors: Horst Boegel
Published: March 2013

Review: 44 refs.
ChemInform Abstract: First‐Principles Calculation of NMR Parameters Using the Gauge Including Projector Augmented Wave Method: A Chemist′s Point of View
Authors: Bonhomme Christian , Gervais Christel , Babonneau Florence , Coelho Cristina , Pourpoint Frederique , Azais Thierry , Ashbrook Sharon E. , Griffin John M. , Yates Jonathan R. , Mauri Francesco , et al. et al.
Published: March 2013

Review: 439 refs.
Copper‐ and Silver‐Mediated Cyanation of Aryl Iodides Using DDQ as Cyanide Source
Authors: Zheng Kui , Yu Peng , Chen Shuyou , Chen Fen , Cheng Jiang
Published: March 2013

A new copper and silver‐mediated cyanation of aryl iodides with DDQ as a cyanide source is achieved, providing nitriles with good yields. This new approach represents a safe method leading to aryl nitriles.
Synthesis of Bi‐doped TiO2 Nanotubes and Enhanced Photocatalytic Activity for Hydrogen Evolution from Glycerol Solution
Authors: Zhao Wenxi , Wang Xitao , Sang Huanxin , Wang Kang
Published: March 2013

Bi‐doped TiO2 nanotubes with variable Bi/Ti ratios were synthesized by hydrothermal treatment in 10 mol·L−1 NaOH (aq.) through using Bi‐doped TiO2 particles derived from conventional sol‐gel method as starting materials. The effects of Bi content on the morphology, textural properties, photo absorption and photocatalytic activity of TiO2 nanotubes were investigated. The scanning electron microscopy (SEM), transmission electron microscopy (TEM), X‐ray diffraction (XRD) and X‐ray photoelectron spectroscopy (XPS) observations of the obtained samples revealed the formation of titanate nanotube structure doped with Bi, which exists as a higher oxidation state than Bi3+. Bi‐doping TiO2 nanotubes exhibited an extension of light absorption into the visible region and improved photocatalytic activities for hydrogen production from a glycerol/water mixed solution as compared with pure TiO2 nanotubes. There was an optimal Bi‐doped content for the photocatalytic hydrogen production, and high content of Bi would retard the phase transition of titanate to anatase and result in morphology change from nanotube to nanobelt, which in turn decreases the photocatlytic activity for hydrogen evolution.
Left Atrial Diameter, Aortic Mean Gradient, and Hemoglobin for Risk Stratification in Patients Undergoing Transcatheter Aortic Valve Implantation
Authors: Gotzmann Michael , Thiessen Anja , Lindstaedt Michael , Mügge Andreas , Ewers Aydan
Published: March 2013

Background

Transcatheter aortic valve implantation (TAVI) is an alternative treatment of severe symptomatic aortic stenosis (AS) in patients with high operative risk. In spite of favorable entire results, long‐term mortality of patients is high.

Hypothesis

The present study aims to identify independent preprocedural risk factors to improve risk stratification in these highly selected patients.

Methods

This prospective study included 202 consecutive patients with severe symptomatic AS and high operative risk (mean logistic European System for Cardiac Operative Risk Evaluation, 22 ± 17%; mean age, 79 ± 6 years; 107 female). Preprocedural comprehensive examinations were performed (laboratory, electrocardiography, echocardiography, cardiac catheterization). All patients received transfemoral or transaxillary TAVI with a CoreValve prosthesis (Medtronic, Minneapolis, MN).

Results

During a follow‐up of 535 ± 333 days, 56 patients (28%) reached the primary study end point (all‐cause mortality). Independent predictors of long‐term mortality were as follows: hemoglobin <12.5 g/dL (hazard risk [HR], 3.62; 95% confidence interval [CI], 2.025–6.468; P < 0.001), aortic mean gradient ≤41 mm Hg (HR, 2.16; 95% CI, 1.272–3.655; P = 0.004), and left atrial diameter > 42 mm (HR, 3.09; 95% CI, 1.588–6.019; P = 0.001). Our risk‐stratification model based on these independent predictors separated patients into 4 groups with high (74%), intermediate (37%), low (18%), and very low (3%) all‐cause mortality.

Conclusions

In patients undergoing TAVI, preprocedural assessment of hemoglobin, aortic mean gradient, and left atrial diameter provides independent prognostic information and therefore contributes to improved risk stratification in TAVI.
Automatic cell cloning assay for determining the clonogenic capacity of cancer and cancer stem‐like cells
Authors: Fedr Radek , Pernicová Zuzana , Slabáková Eva , Straková Nicol , Bouchal Jan , Grepl Michal , Kozubík Alois , Souček Karel
Published: March 2013

The clonogenic assay is a well‐established in vitro method for testing the survival and proliferative capability of cells. It can be used to determine the cytotoxic effects of various treatments including chemotherapeutics and ionizing radiation. However, this approach can also characterize cells with different phenotypes and biological properties, such as stem cells or cancer stem cells. In this study, we implemented a faster and more precise method for assessing the cloning efficiency of cancer stem‐like cells that were characterized and separated using a high‐speed cell sorter. Cell plating onto a microplate using an automatic cell deposition unit was performed in a single‐cell or dilution rank mode by the fluorescence‐activated cell sorting method. We tested the new automatic cell‐cloning assay (ACCA) on selected cancer cell lines and compared it with the manual approach. The obtained results were also compared with the results of the limiting dilution assay for different cell lines. We applied the ACCA to analyze the cloning capacity of different subpopulations of prostate and colon cancer cells based on the expression of the characteristic markers of stem (CD44 and CD133) and cancer stem cells (TROP‐2, CD49f, and CD44). Our results revealed that the novel ACCA is a straightforward approach for determining the clonogenic capacity of cancer stem‐like cells identified in both cell lines and patient samples. © 2013 International Society for Advancement of Cytometry