First Report of Pomegranate Wilt Caused by Ceratocystis fimbriata in Yunnan, China
Authors: Huang Q. , Zhu Y. Y. , Chen H. R. , Wang Y. Y. , Liu Y. L. , Lu W. J. , Ruan X. Y. 
Published: May 2007

Pomegranate (Punica granatum Linn.) is an important fruit crop in Yunnan Province, China. Recently, older pomegranate bushes in Mengzi County, Yunnan began dying. Initial symptoms were yellowing and wilting of leaves on one to several branches, followed by sudden death of the bush within 3 to 4 weeks. Roots of diseased bushes appeared brown to black, and irregularly shaped lesions were observed when the bark was removed. A species of Ceratocystis was consistently isolated from discolored roots, stem, and branch tissues from wilted bushes on potato dextrose agar (PDA) and was identified as Ceratocystis fimbriata Ellis & Halst, based on the morphology of perithecia, ascospores, conidia, and conidiophores. Perithecia were black with a globose base (130 to 300 μm) and a long neck (450 to 800 μm). Ascospores exuded from the apex of the perithecium neck in a long coil and were small, hyaline, and hat-shaped (3.8 to 5.0 μm long × 2.3 to 4.0 μm wide). Conidiophores were septate and hyaline to dark greenish brown. Hyaline conidia, 8 to 17 μm long × 6 to 15 μm wide, were usually produced in chains of 10 or more. Thick-walled endoconidia were globose to oval, olive brown, and 8 to 20 μm in diameter. Because of the increasing occurrence of the disease, surveys of the main pomegranate production areas in Mengzi County, including Xinan, Duofale, Caoba, Hongzhai, and Shilipu townships, were conducted from 10 to 20 August 2002. The disease was detected in 17 of 50 plantings surveyed. Disease was more severe in older plantings than in younger plantings. Disease incidence was 1% in 1- to 5-year-old bushes, 3.6% in 6- to 10-year-old bushes, and 6% in bushes more than 10 years old. Scolytid beetles were occasionally found on bushes, but we were unable to isolate the fungus from them as has been reported (1). Inoculations with an isolate of C. fimbrata were made by inserting mycelium with perithecia from 12-day-old cultures growing on PDA into root wounds made with a sterile scalpel on five pomegranate plants and then covering the wounds with Parafilm. Sterile medium was placed in an equal number of wounded bushes to serve as controls. Fourteen days later, symptoms began to appear in two bushes, and 5 days later, all bushes exhibited symptoms. No symptoms were observed on control bushes. The first visible symptom was a small area of blackened tissue near the point of inoculation. Lesions expanded slowly, but they expanded more rapidly upward than downward. The fungus was reisolated on PDA from roots of all artificially inoculated bushes. C. fimbriata has been previously reported as the cause of pomegranate wilt in India (2); however, to our knowledge, this is the first report of C. fimbriata on pomegranate in China. Because environmental conditions which favor the pathogen (temperatures ranging from 18 to 30°C and frequent rains) typically occur in many areas during late spring and summer, the disease has the potential to seriously impact pomegranate production in China.

References: (1) Y. M. Somasekhara. Plant Dis. 83:400, 1999. (2) Y. M. Somasekhara, et al. Res. Crops 1(1):63, 2000.