bluuebunny/crossref_metadata_2025 huggingface dataset:
GPR30‐mediated estrogenic regulation of actin polymerization and spatial memory involves SRC‐1 and PI3K‐mTORC2 in the hippocampus of female mice
Authors: Zhang Yuan‐Yuan , Liu Meng‐Ying , Liu Zhi , Zhao Ji‐Kai , Zhao Yan‐Gang , He Li , Li Wei , Zhang Ji‐Qiang
Published: February 2019

Aims

The G‐protein‐coupled estrogen receptor GPR30 (also referred to as GPER) has been implicated in the estrogenic regulation of hippocampal plasticity and spatial memory; however, the molecular mechanisms are largely unclear.

Methods

In this study, we initially examined the levels of GPR30 in the hippocampus of postnatal, ovariectomy (OVX)‐ and letrozole (LET)‐treated female mice. Under G1, G15, and/or OVX treatment, the spatial memory, spine density, levels of ERα, ERβ, and SRC‐1, selected synaptic proteins, mTORC2 signals (Rictor and p‐AKT Ser473), and actin polymerization dynamics were subsequently evaluated. Furthermore, G1, G15, and/or E2 combined with SRC‐1 and/or PI3K inhibitors, actin cytoskeleton polymerization modulator JPK, and CytoD treatments were used to address the mechanisms that underlie GPR30 regulation in vitro. Finally, mTORC2 activator A‐443654 (A4) was used to explore the role of mTORC2 in GPR30 regulation of spatial memory.

Results

The results showed that high levels of GPR30 were detected in the adult hippocampus and the levels were downregulated by OVX and LET. OVX induced an impairment of spatial memory, and changes in other parameters previously described were reversed by G1 and mimicked by G15. Furthermore, the E2 effects on SRC‐1 and mTORC2 signals, synaptic proteins, and actin polymerization were inhibited by G15, whereas G1 effects on these parameters were inhibited by the blockade of SRC‐1 or PI3K; the levels of synaptic proteins were regulated by JPK and CytoD. Importantly, G15‐induced actin depolymerization and spatial memory impairment were rescued by mTORC2 activation with A4.

Conclusions

Taking together, these results demonstrated that decreased GPR30 induces actin depolymerization through SRC‐1 and PI3K/mTORC2 pathways and ultimately impairs learning and memory, indicating its potential role as a therapeutic target against hippocampus‐based, E2‐related memory impairments.
3‐dimensional visualization of implant‐tissue interface with the polyethylene glycol associated solvent system tissue clearing method
Authors: Yi Yating , Men Yi , Jing Dian , Luo Wenjing , Zhang Shiwen , Feng Jian Q. , Liu Jin , Ge Woo‐Ping , Wang Jun , Zhao Hu
Published: February 2019

Objectives

Dental implants are major treatment options for restoring teeth loss. Biological processes at the implant‐tissue interface are critical for implant osseointegration. Superior mechanical properties of the implant constitute a major challenge for traditional histological techniques. It is imperative to develop new technique to investigate the implant‐tissue interface.

Materials and methods

Our laboratory developed the polyethylene glycol (PEG)‐associated solvent system (PEGASOS) tissue clearing method. By immersing samples into various chemical substances, bones and teeth could be turned to transparent with intact internal structures and endogenous fluorescence being preserved. We combined the PEGASOS tissue clearing method with transgenic mouse line and other labelling technique to investigate the angiogenesis and osteogenesis processes occurring at the implant‐bone interface.

Results

Clearing treatment turned tissue highly transparent and implant could be directly visualized without sectioning. Implant, soft/hard tissues and fluorescent labels were simultaneously imaged in decalcified or non‐decalcified mouse mandible samples without disturbing their interfaces. Multi‐channel 3‐dimensional image stacks at high resolution were acquired and quantified. The processes of angiogenesis and osteogenesis surrounding titanium or stainless steel implants were investigated.

Conclusions

Both titanium and stainless steel implants support angiogenesis at comparable levels. Successful osseointegration and calcium precipitation occurred only surrounding titanium, but not stainless steel implants. PEGASOS tissue clearing method provides a novel approach for investigating the interface between implants and hard tissue.
The Canadian alcopop tragedy should trigger evidence‐informed revisions of federal alcohol regulations
Authors: Paradis Catherine , April Nicole , Cyr Claude , Morin Réal , Niquette Manon
Published: February 2019

On 1 March 2018, a 14‐year‐old girl was found lifeless in a stream behind her high school after having consumed FCKDUP—a beverage containing 11.9% alcohol and sold in 568 mL cans—during her lunch hour. Following her death, the Canadian government took actions at ministerial and parliamentary levels by seeking experts’ advice to better regulate highly sweetened alcoholic beverages, otherwise referred to as ‘alcopops’. We suggest that the Canadian government uses the work surrounding the alcopop tragedy as an opportunity to make significant amendments and revisions of federal alcohol regulations.
Independent role of neonatal seizures in subsequent neurological outcomes: a population‐based study
Authors: Oh Ahyuda , Thurman David J , Kim Hyunmi
Published: February 2019

Aim

This population‐based study aimed to estimate the impact of neonatal seizures on subsequent neurological outcomes, regardless of underlying etiology.

Method

We performed a retrospective cohort study (1st January 2009–31st December 2014), using a USA nationwide claims database. Newborn infants enrolled in 2009 were followed for up to 6 years. Neonatal seizures were identified by combining the International Classification of Diseases, Ninth Revision, Clinical Modification diagnosis code of 779.0 (convulsions in newborn), procedure codes of electroencephalogram and brain imaging, and antiepileptic drugs claims. Cox regression models were built to estimate the independent impact of neonatal seizures on developing epilepsy, intellectual disability, psychiatric/behavioral disorders, and headache.

Results

Out of 490 071 newborn infants (251 850 males [51.4%], 238 221 females [48.6%]), 800 neonatal seizure cases were identified. After controlling for sex, birthweight, preterm birth status, and underlying etiology, neonates with seizures were more likely to have epilepsy (hazard ratio=32.7; 95% confidence interval [CI]=27.7−38.7; p<0.001), intellectual disability (hazard ratio=2.0; 95% CI=1.8−2.3; p<0.001), and headache (hazard ratio=1.6; 95% CI=1.1−2.2; p=0.013) than those without seizures.

Interpretation

Observed covariates being equal, seizures in neonates appeared to play a significant role in developing epilepsy, intellectual disability, and headache. The findings showed a detrimental impact of the event in the very early life on neurological outcomes in later life.

What this paper adds

Seizures had their own impact on the development of adverse neurological outcomes.

The magnitude of impact was quite large in epilepsy.
Response of in vitro obtained oil palm and interspecific OxG hybrids to inoculation with Phytophthora palmivora
Authors: Ávila‐Méndez Kelly , Avila‐Diazgranados Rodrigo , Pardo Alonso , Herrera Mariana , Sarria Greicy , Romero Hernan Mauricio
Published: February 2019

Bud rot (BR) disease caused by Phytophthora palmivora is the most devastating disease in oil palm cultivation in America. Oil palms that survived BR epidemics were found in areas highly devastated by the disease; these palms were introduced into Cenipalma's in vitro micropropagation (cloning programme). A severity scale was developed for in vitro palms from five ortets inoculated with two different P. palmivora isolates. Then, eight ortets of Elaeis guineensis and two ortets of the OxG interspecific hybrid were evaluated in two inoculation trials under chamber growth conditions. The clone performance response was consistent with that reported in the field for the corresponding ortets, and two contrasting ortets, one susceptible (ortet 57) and one resistant (ortet 34) were identified. We monitored and compared defence responses to P. palmivora in the contrasting ortets. An increase in reactive oxygen species (ROS) production after inoculation with the pathogen was observed, with higher accumulation of H2O2 in the resistant plants. Catalase (CAT) activity in resistant plants increased after inoculation with the pathogen from 24 hr post‐infection (hpi) and remained high during the observation time. In the susceptible ortets, there was a significant increase on catalase activity only at 48 hpi. Peroxidase (POS) activity increased in clones from both susceptible and resistant ortets, but the increase was much greater in the susceptible ones. Phenylalanine ammonia lyase activity increased in response to inoculation, and these increases were greater in clones of the susceptible ortet than in clones of the resistant ortet.
Classification of radiologically isolated syndrome and clinically isolated syndrome with machine‐learning techniques
Authors: Mato‐Abad V. , Labiano‐Fontcuberta A. , Rodríguez‐Yáñez S. , García‐Vázquez R. , Munteanu C. R. , Andrade‐Garda J. , Domingo‐Santos A. , Galán Sánchez‐Seco V. , Aladro Y. , Martínez‐Ginés M. L. , Ayuso L. , Benito‐León J.
Published: February 2019

Background and purpose

The unanticipated detection by magnetic resonance imaging (MRI) in the brain of asymptomatic subjects of white matter lesions suggestive of multiple sclerosis (MS) has been named radiologically isolated syndrome (RIS). As the difference between early MS [i.e. clinically isolated syndrome (CIS)] and RIS is the occurrence of a clinical event, it is logical to improve detection of the subclinical form without interfering with MRI as there are radiological diagnostic criteria for that. Our objective was to use machine‐learning classification methods to identify morphometric measures that help to discriminate patients with RIS from those with CIS.

Methods

We used a multimodal 3‐T MRI approach by combining MRI biomarkers (cortical thickness, cortical and subcortical grey matter volume, and white matter integrity) of a cohort of 17 patients with RIS and 17 patients with CIS for single‐subject level classification.

Results

The best proposed models to predict the diagnosis of CIS and RIS were based on the Naive Bayes, Bagging and Multilayer Perceptron classifiers using only three features: the left rostral middle frontal gyrus volume and the fractional anisotropy values in the right amygdala and right lingual gyrus. The Naive Bayes obtained the highest accuracy [overall classification, 0.765; area under the receiver operating characteristic (AUROC), 0.782].

Conclusions

A machine‐learning approach applied to multimodal MRI data may differentiate between the earliest clinical expressions of MS (CIS and RIS) with an accuracy of 78%.
Characterization of nitrogen transformation dynamics in alfalfa and red clover and their mixture silages
Authors: Dong Zhihao , Chen Lei , Li Junfeng , Yuan Xianjun , Shao Tao
Published: February 2019

The objective of this experiment was to characterize the dynamics of nitrogen (N) transformations in alfalfa and red clover and their mixture during ensiling. Alfalfa, red clover and a mixture of them (1:1 on fresh weight) were ensiled for 3, 5, 7, 14 and 30 days. The fermentation qualities and dynamic change of N components of the three silages were determined. Red clover and mixed silages showed better fermentation qualities, indicated by lower (p < 0.05) pH value, higher (p < 0.05) lactic acid concentration compared with alfalfa silage after ensiling. Retarded N transformation was observed in red clover and mixed silages, as evidenced by lower (p < 0.05) free amino acid concentrations at all intervals of ensiling, higher (p < 0.05) peptide N and lower (p < 0.05) ammonia N concentrations after 14 days of ensiling as opposed to alfalfa silage. Mixing red clover apparently delayed the degradation process of alfalfa protein in mixed silage, as demonstrated by no significant difference in non‐protein N (NPN) concentrations in comparison with pure red clover silage at most intervals of ensiling. The results of this study, from the perspective of N transformation, confirmed the lower rate of protein degradation in red clover in contrast to alfalfa during ensiling and potentially provide an effective strategy to reduce protein loss for alfalfa silage production.
A small, distant war? Historiographical reflections on the Malayan Emergency
Authors: Keo Bernard Z.
Published: February 2019

The Malayan Emergency (1948–1960), one of the earliest conflicts of the Cold War, remains understudied despite the voluminous literature produced by specialists on the topic. Most of these works have focused primarily on unpacking the lessons offered by the Emergency in the realm of counterinsurgency at the expense of explorations of the conflict as a lived experience. This article explores how the historiography on the Emergency has changed over time, focusing on how it has been studied primarily in terms of its political and military implications with the key debate emerging over which strategy and tactics ultimately led to British victory. One school of thought argues that success was a consequence of British forces embarking to win “hearts and minds” rather than focusing on traditional warfare. Newer works have challenged this discourse, suggesting instead that the Emergency was won through strict and oftentimes violent “population control.” While these approaches have been integral to furthering understandings of the Emergency, the overwhelming focus on the military aspects of the Emergency has resulted in a number of gaps in the field, which this article highlights before concluding with discussions on how these gaps might be addressed.
Micro‐CT evaluation of the removal of root fillings using the ProTaper Universal Retreatment system supplemented by the XP‐Endo Finisher file
Authors: Aksel H. , Küçükkaya Eren S. , Askerbeyli Örs S. , Serper A. , Ocak M. , Çelik H. H.
Published: February 2019

Aim

To compare the removal of root fillings in extracted teeth using the ProTaper Universal Retreatment system (PTUR) followed by a supplementary preparation with the XP‐Endo Finisher file.

Methodology

The mesiobuccal root canals of 30 extracted mandibular first molars were instrumented with ProTaper Universal NiTi files up to F2 and filled with one of the following sealers using a single‐cone technique (n = 10): AH Plus, NeoMTA Plus and EndoSequence BC. The root fillings were removed using the PTUR system with additional apical preparation using ProTaper F2 and F3 files. Then, an additional preparation with an XP‐Endo Finisher file was performed. The samples were scanned using micro‐CT before and after retreatment and again after the use of the XP‐Endo Finisher to assess the volume of remaining filling material. Data were analysed by Kruskal–Wallis and Friedman's two‐way analysis of variance tests with Bonferroni correction.

Results

No significant difference in the initial filling volume was observed amongst the groups (P > 0.05). The amount of filling materials in each group decreased significantly after retreatment with rotary files (P < 0.05). After initial retreatment, the volume of the remaining filling amongst the groups was similar (P > 0.05). Additional preparation with the XP‐Endo Finisher improved the removal of filling materials regardless of the sealer type (P < 0.05).

Conclusions

In the mesiobuccal root canals of extracted mandibular first molars, additional preparation using an XP‐Endo Finisher file, after using the PTUR system and F3 ProTaper instruments, enhanced the removal of filling materials from root canals.
Self‐signal‐on fluorescent colorimetric protocol for rapid authentication of horsemeat adulterated beef samples with functional designed probes
Authors: Qin Panzhu , Qiao Dongqing , Gao Yan , Yao Li , Lu Jianfeng , Xu Jianguo , Chen Wei
Published: February 2019

Rapid authentication of meat adulteration is of great importance for food safety. A new FRET‐based self‐signal‐on fluorescent colorimetric PCR approach for rapid, sensitive and specific identification of horsemeat ingredient has been developed. A specific hairpin probe was designed and integrated with the forward primer for the detection of horsemeat. This specific hairpin sequence was designed to generate fluorescent signal only when primers are incorporated into the formation of double‐stranded PCR product. The fluorescent signal of the PCR product is in a linear relationship with the proportion of horsemeat ingredient, and could be used for the quantitative detection of horsemeat ingredient in meat products without any post‐treatment or analysis of the amplicons. Based on the fluorescent signal intensity, rapid and convenient detection of horsemeat ingredient in beef products was successfully achieved with satisfied specificity and sensitivity.
Changes of peach juices during the shelf‐life and their in vitro effect on glycolipid digestion and neurotransmitter metabolism
Authors: Nowicka Paulina , Teleszko Mirosława , Wojdyło Aneta
Published: February 2019

In this study, the polyphenols content, α‐amylase, α‐glucosidase and cholinesterase‐inhibiting activities of cloudy peach (Prunus persica) juices, were measured immediately after thermal treatment and after storage (6 months at 4 and 30 °C). The study showed that some cultivars were more useable for industry than others. Due to the high content of polyphenols and a high ability to inhibit enzymes linked to type 2 diabetes and Alzheimer's disease, noteworthy are the juices obtained from ‘Redheaven early’ and ‘Redheaven’ cultivars. The content of total phenolics ranged from 1109.7 to 390.7 mg/L of juice, and the dominant fraction was polymeric procyanidins. Following 6 months of storage a significant change was observed in the content of polyphenols, especially flavonols and anthocyanins. The data compiled from the conducted study indicate that peach juice could be useful for the juice industry as a promising source of bioactive compounds.
Optimising chitosan–pectin hydrogel beads containing combined garlic and holy basil essential oils and their application as antimicrobial inhibitor
Authors: Torpol Kittikoon , Sriwattana Sujinda , Sangsuwan Jurmkwan , Wiriyacharee Pairote , Prinyawiwatkul Witoon
Published: February 2019

Chitosan–pectin hydrogel beads that trap and release the maximal amount of combined garlic and holy basil essential oils to inhibit food microorganisms were developed based on the central composite design, with chitosan (0.2–0.7% w/v), pectin (3.5–5.5% w/v) and calcium chloride (CaCl2) (5.0–20.0% w/v) contents. The optimal bead consisted of 0.3–0.6% w/v chitosan, 3.9–5.1% w/v pectin and 8.0–17.0% w/v CaCl2, which had a high encapsulation efficiency (62.16–79.06%) and high cumulative release efficiency (31.55–37.81%) after storage at 5 °C for 15 days. Optimal hydrogel beads were packed into a cellulose bag to evaluate antimicrobial activity by the disc volatilisation method. The beads inhibited Bacillus cereus, Clostridium perfringens, Escherichia coli, Pseudomonas fluorescens, Listeria monocytogenes and Staphylococcus aureus but did not affect Lactobacillus plantarum and Salmonella Typhimurium. The oil‐containing beads could potentially be applied in food packaging to inhibit the mentioned microorganisms.
Chinese seniors’ attitudes towards government responsibility for social welfare: Self‐interest, collectivism orientation and regional disparities
Authors: Yang Kun , Peng Huamin , Chen Jia
Published: February 2019

Government responsibility for social welfare remains a significant issue in the field of social welfare. Public welfare attitudes not only refer to the social needs of members of society, but also are viewed as the basis for government responsibility for social welfare. Data used in this study came from the Moderate Universalist Social Welfare Survey with a final sample of 1,166 seniors from four Chinese cities in 2012. This study examined seniors’ attitudes towards government responsibility for providing specific welfare and mixed welfare. It concluded that seniors’ welfare attitudes share the traits of self‐interest in general. Seniors’ perception of social rights and cities of residence were two important factors associated with welfare attitudes in both aspects of specific and mixed welfare. The stronger seniors’ social rights perception was the more favourable were the attitudes they held towards government responsibility for social welfare, indicating the collectivism orientation of their welfare attitudes.
Variables which predict maternal self‐efficacy: A hierarchical linear regression analysis
Authors: Yap Dorcas Fen‐Fung , Nasir Nasriah , Tan Karen S. M. , Lau Lily H. S.
Published: February 2019

Background

Children with developmental disabilities tend to develop challenging behaviours. Parenting programmes that focus on behaviour management may help parents address these difficult behaviours by increasing parenting self‐efficacy. However, the literature on parenting self‐efficacy is still limited due to measurement variability in conceptualization and operationalization of the construct, and heavy reliance on cross‐sectional data.

Method

This study utilized hierarchical linear regression to examine the predictors of parenting self‐efficacy in 284 mothers who attended a parenting programme in a hospital's Department of Child Development in Singapore.

Results

Our model was able to explain 37% of variance in parenting self‐efficacy. After controlling for general stress and specific parenting hassles, the programme was still found to be effective in improving mothers’ parenting self‐efficacy.

Conclusions

Results are discussed in terms of their implications for developing a model for parenting self‐efficacy, and possible improvements to be made on the parenting programme.
A comparative morphometric study of sensory capacity in geometrid moths
Authors: Javoiš Juhan , Davis Robert B. , Tammaru Toomas
Published: February 2019

Diet breadth and the degree of capital breeding have been established as major determinants of species‐specific ecology of herbivorous insects. Both of these variables are related to resource foraging and therefore can be expected to have effects on sensory capacity. However, such effects have remained poorly studied, let alone in phylogenetically explicit multi‐species comparisons. We contribute to filling this gap in a study of 60 species of geometrid moths (Lepidoptera: Geometridae), using adult head measures (eye size, antennal length and shape, forehead width) as indices of sensory capacity. When controlled for body size, eyes of the more capital breeding species (i.e. those with low contribution of adult feeding to reproduction) were found to be smaller, and female antennae shorter, than in income breeders. Feathery (vs simple filiform) male antennae were more frequently present in the capital breeders and in larger species. Regarding diet breadth, generalist species were found to have relatively wider male foreheads than specialists. The results suggest that (a) breeding strategy rather than diet breadth predicts sensory capacity in geometrids, (b) capital breeding (vs income breeding) is related to low sensory capacity in females, and (c) in contrast, males of the capital breeding species have evolved towards elevated olfactory capacity.
Educational Differences in Cognitive Life Expectancy Among Older Adults in Brazil
Authors: Andrade Flavia Cristina Drumond , Corona Ligiana Pires , de Oliveira Duarte Yeda Aparecida
Published: February 2019

Objectives

To investigate the differences in life expectancy with and without cognitive impairment (CI) by educational levels and sex in Brazil.

Design

Longitudinal observational study.

Setting

The sample was drawn from three waves (2000, 2006, and 2010) of the Health, Well‐Being, and Aging Study (Saúde, bem‐estar e envelhecimento; SABE) collected in São Paulo, Brazil.

Participants

Adults aged 60 years and older (N = 2116).

Measurements

Educational levels were estimated in the baseline wave (2000), cognition was assessed in all waves, and mortality data were obtained through the state and municipal mortality system in Brazil. Interpolation of Markov chain methods was used to estimate life expectancy with and without CI by education and sex.

Results

Life expectancy without CI at the age of 60 years was 13.0 years among men with no education and 17.6 years among their counterparts with 8 years of schooling. On the other hand, life expectancy with CI was higher among men with no education than those with more education (3.2 and 0.6 years, respectively). Among 60‐year‐old women without education, life expectancy without CI reached 16.2 years, but it was considerably higher among more educated women (22.7 years). Life expectancy with CI reached 4.5 years among women aged 60 years with no education, vs 1.0 year among women with 8 years of schooling.

Conclusions

Adults older than 60 years with no education live shorter lives and with longer periods of CI than those with education. Women in São Paulo live longer lives than men, but they live with CI for a greater number of years.
Serum biotin and zinc in male androgenetic alopecia
Authors: El‐Esawy Fatma Mohamed , Hussein Mohamed Saber , Ibrahim Mansour Amira
Published: February 2019

Background

Male androgenetic alopecia (MAGA) is caused by the conversion of the terminal to vellus hair. Zinc is one of the most studied trace elements in hair disorders and biotin is one of the most prescribed supplement for its treatment.

Objectives

The study aimed to evaluate serum zinc and biotin levels in MAGA patients to answer the question if there is a value to add zinc or biotin as a supplements in the MAGA treatment.

Patients and Methods

Sixty MAGA patients and 60 age, sex, and body mass index‐matched healthy volunteers were included. We measured serum biotin and zinc in all participants.

Results

Zinc (µg/dL) was lower significantly in patients compared to controls (P = 0.01), suboptimal biotin (ng/L) levels were in patients, and within normal values in controls (P = 0.01). A positive significant correlation was found between serum zinc and serum biotin (r = 0.489, P = 0.001). Serum zinc and biotin showed a nonsignificant correlation with age and disease duration. A non‐significant relation was obtained between the MAGA grades, and zinc (P = 0.485) and biotin levels (P = 0.367).

Conclusions

Serum zinc showed subnormal value and adding zinc supplement in MAGA treatment is recommended. Serum biotin showed a suboptimal level in MAGA patients that is not correlated with patients’ age or disease severity. Biotin supplement in MAGA treatment may add value to hair quality and texture. We recommend future biotin evaluation in serum combined with its metabolites in MAGA patients’ urine.
18F‐Florbetaben PET/CT to Assess Alzheimer's Disease: A new Analysis Method for Regional Amyloid Quantification
Authors: Alongi Pierpaolo , Sardina Davide Stefano , Coppola Rosalia , Scalisi Salvatore , Puglisi Valentina , Arnone Annachiara , Raimondo Giorgio Di , Munerati Elisabetta , Alaimo Valerio , Midiri Federico , Russo Giorgio , Stefano Alessandro , Giugno Rosalba , Piccoli Tommaso , Midiri Massimo , Grimaldi Luigi M.E.
Published: February 2019

BACKGROUND AND PURPOSE

While AD can be definitively confirmed by postmortem histopathologic examination, in vivo imaging may improve the clinician's ability to identify AD at the earliest stage. The aim of the study was to test the performance of amyloid PET using new processing imaging algorithm for more precise diagnosis of AD.

METHODS

Amyloid PET results using a new processing imaging algorithm (MRI‐Less and AAL Atlas) were correlated with clinical, cognitive status, CSF analysis, and other imaging. The regional SUVR using the white matter of cerebellum as reference region and scores from clinical and cognitive tests were used to create ROC curves. Leave‐one‐out cross‐validation was carried out to validate the results.

RESULTS

Forty‐four consecutive patients with clinical evidence of dementia, were retrospectively evaluated. Amyloid PET scan was positive in 26/44 patients with dementia. After integration with 18F‐FDG PET, clinical data and CSF protein levels, 22 of them were classified as AD, the remaining 4 as vascular or frontotemporal dementia. Amyloid and FDG PET, CDR 1, CSF Tau, and p‐tau levels showed the best true positive and true negative rates (amyloid PET: AUC = .85, sensitivity .91, specificity .79). A SUVR value of 1.006 in the inferior frontal cortex and of 1.03 in the precuneus region was the best cutoff SUVR value and showed a good correlation with the diagnosis of AD. Thirteen of 44 amyloid PET positive patients have been enrolled in clinical trials using antiamyloid approaches.

CONCLUSIONS

Amyloid PET using SPM‐normalized SUVR analysis showed high predictive power for the differential diagnosis of AD.
Chalcone analogues: Synthesis, activity against Meloidogyne incognita, and in silico interaction with cytochrome P450
Authors: Silva Fabíola J. , Campos Vicente P. , Oliveira Denilson F. , Gomes Vanessa A. , Barros Aline F. , Din Zia Ud , Rodrigues‐Filho Edson
Published: February 2019

To contribute the development of new products to control plant‐parasitic nematodes, 12 chalcone analogues were synthesized and screened for activity against Meloidogyne incognita. Three caused mortality greater than negative controls in second‐stage juvenile M. incognita, with values varying from 19.9% to 100%. The most active chalcone analogue was (1E,4E)‐1,5‐di(4‐nitrophenyl)‐2‐butylpenta‐1,4‐dien‐3‐one (compound 6), which had an LC50 value of 41 µg/ml. Under the same conditions, the commercial nematicide Carbofuran® (2,2‐dimethyl‐2,3‐dihydro‐1‐benzofuran‐7‐yl methylcarbamate) presented an LC50 equal to 101 µg/ml. When this chalcone analogue was applied to tomato plants infested with M. incognita, reductions in the numbers of galls and eggs of 51% and 68% were observed, respectively. According to in silico studies, the enzyme target of compound 6 in M. incongita is cytochrome P450, which is important for the oxidation of several substances in the nematode. Therefore, compound 6 is potentially useful for the development of new products to control M. incongita.
Ontogeny of food grasping in mouse lemurs: behavior, morphology and performance
Authors: Boulinguez‐Ambroise G. , Zablocki‐Thomas P. , Aujard F. , Herrel A. , Pouydebat E.
Published: February 2019

In primates, grasping constitutes a vital function involved in many behaviors. Anatomical specializations of the prehensile extremities originated early‐on in their evolution. However, the precise functional and ecological contexts that have driven this evolution remain unclear. Interspecific comparative studies show that a given morphology can induce different grasping behaviors and also that a given behavior can be performed using different morphological structures. In this context, an intraspecific ontogenetic approach offers the opportunity to describe the relations between patterns in grasping behavior and patterns of grasping morphology. We quantified manual grasping strategies and the associated morphometric (i.e. segments lengths) and performance (i.e. pull strength) traits for both limbs during the development of a small arboreal primate, the gray mouse lemur (Microcebus murinus). Our results show an early onset of grasping in young mouse lemurs. Moreover, younger individuals had relative longer hindlimbs (i.e. tibia and metatarsus) allowing them to have near‐maximal levels of grasping strength. This very fast development despite an incomplete neuromuscular development highlights the importance of this grasping function directly after birth.
Year‐to‐year variability of epifaunal assemblages on a mobile hard substrate—Case study from high latitudes
Authors: Balazy Piotr , Kuklinski Piotr
Published: February 2019

Past polar regional studies on temporal trends of benthic systems have mostly been performed on soft bottom communities. Fewer studies have dealt with hard bottom, and none have been performed with mobile hard substrates, yet these are common, abundant and host high biodiversity—usually higher than similar substrates even if the latter were larger in size. In this study, we analysed the variability of epifaunal assemblages on a mobile hard substrate (Buccinum sp. and Littorina sp. shells utilized by hermit crab Pagurus pubescens) collected annually during 5 years (2005–2009) in Spitsbergen Island (Svalbard Archipelago, 78° N) and Northern Norway (68° N). The epifaunal assemblages were variable over the observed period, with differences in abundance, diversity (S, H′) and assemblage structure. Among the species that contributed the most to the observed differences were barnacle Semibalanus balanoides, serpulid polychaetes (Circeis armoricana, Spirobranchus triqueter) and foraminiferans (Discorbis rosacea, Lobatula lobatula), which dominate the assemblages. Analyses indicated that epifauna found on hermit crab shells is mostly influenced by environmental effects linked to NAO, its seasonal values (esp. winter), sometimes lagged. Strong changes were coinciding with a NAO phase shift. Years with positive NAO tend do have higher number of species, abundance and diversity. Differences between Spitsbergen and Northern Norway are most likely driven by geographic setting of the sites and its implications (e.g., hydrography). By exploring the sensitivity to large‐scale climate variability and its direct consequences, the study helps assess the potential of environment monitoring through hermit crabs epifauna. It provides also a baseline data allowing comparative, or after disturbance, studies in future.
Comparative phylogeography of trans‐Andean freshwater fishes based on genome‐wide nuclear and mitochondrial markers
Authors: Rincon‐Sandoval Melissa , Betancur‐R Ricardo , Maldonado‐Ocampo Javier A.
Published: February 2019

The Neotropical region represents one of the greatest biodiversity hot spots on earth. Despite its unparalleled biodiversity, regional comparative phylogeographic studies are still scarce, with most focusing on model clades (e.g. birds) and typically examining a handful of loci. Here, we apply a genome‐wide comparative phylogeographic approach to test hypotheses of codiversification of freshwater fishes in the trans‐Andean region. Using target capture methods, we examined exon data for over 1,000 loci combined with complete mitochondrial genomes to study the phylogeographic history of five primary fish species (>150 individuals) collected from eight major river basins in Northwestern South America and Lower Central America. To assess their patterns of genetic structure, we inferred genealogical concordance taking into account all major aspects of phylogeography (within loci, across multiple genes, across species and among biogeographic provinces). Based on phylogeographic concordance factors, we tested four a priori biogeographic hypotheses, finding support for three of them and uncovering a novel, unexpected pattern of codiversification. The four emerging inter‐riverine patterns are as follows: (a) Tuira + Atrato, (b) Ranchería + Catatumbo, (c) Magdalena system and (d) Sinú + Atrato. These patterns are interpreted as shared responses to the complex uplifting and orogenic processes that modified or sundered watersheds, allowing codiversification and speciation over geological time. We also find evidence of cryptic speciation in one of the species examined and instances of mitochondrial introgression in others. These results help further our knowledge of the historical geographic factors shaping the outstanding biodiversity of the Neotropics.
Retention of a chromosomal inversion from an anadromous ancestor provides the genetic basis for alternative freshwater ecotypes in rainbow trout
Authors: Arostegui Martin C. , Quinn Thomas P. , Seeb Lisa W. , Seeb James E. , McKinney Garrett J.
Published: February 2019

Migratory behaviour patterns in animals are controlled by a complex genetic architecture. Rainbow trout (Oncorhynchus mykiss) is a salmonid fish that spawns in streams but exhibits three primary life history pathways: stream‐resident (fluvial), lake‐migrant (adfluvial) and ocean‐migrant (anadromous). Previous studies examining fluvial and anadromousO. mykisshave identified several genes associated with life history divergence including the presence of an inversion complex within chromosome 5 (Omy05) that appears to maintain a suite of linked genes controlling migratory behaviour. However, adfluvial trout are migratory without being anadromous, and the genetic basis for this life history has not been investigated from evolutionary perspectives. We sampled wild, native nonanadromous rainbow trout occupying connected stream and lake habitats in a southwest Alaskan watershed to determine whether these fish exhibit genetic divergence between fluvial and adfluvial ecotypes, and whether that divergence parallels that documented in fluvial and anadromousO. mykiss. Data from restriction site‐associated DNA (RAD) sequencing revealed an association between frequencies of both the Omy05 inversion complex and other single nucleotide polymorphisms (SNPs) with habitat type (stream or lake), supporting the genetic divergence of fluvial and adfluvial individuals in sympatry. The presence of a genetic basis for migration into lakes, analogous to that documented for anadromy, indicates that the adfluvial ecotype must be recognized separately from the fluvial form ofO. mykisseven though neither is anadromous. These results highlight the genetic architecture underlying migration and the importance of chromosomal inversions in promoting and sustaining intraspecific diversity.
A culture of control?
Authors: Hawick Lorraine , Thomson Alan , Cleland Jennifer
Published: February 2019

The authors respond to Zeeman et al's analysis of how students use their time, suggesting it would be valuable to shift from quantitative to qualitative measures.
Age of Menarche, Adolescent Sexual Intercourse and Schooling Attainment of Women
Authors: Huang Jian , Groot Wim , Sessions John G. , Tseng Yinyen
Published: February 2019

We use data from the 1970 British Cohort Study to measure the effect of adolescent sexual intercourse on female schooling attainment. We emphasize the appropriate use of menarcheal age as an instrumental variable (IV) for early intercourse. Our analysis suggests that developmental trajectories vary with menarcheal age and, therefore, capturing variations in individual cognitive capacities induced by pubertal timing is crucial for the validity of the IV identification strategy. Our empirical results indicate that adolescent sexuality reduces full‐time education by approximately one year. Given that 37 percent of females in our data exited virginity in adolescence, the aggregate loss of human capital as measured by average years of female schooling could be up to one‐third of a year.
External Fixator‐assisted Ulnar Osteotomy: A Novel Technique to Treat Missed Monteggia Fracture in Children
Authors: Wang Qiang , Du Meng‐meng , Pei Xin‐jian , Luo Jun‐zhong , Li Ya‐zhou , Liu Yu‐chang , Wang Xuan , Cao Jin‐chao , Han Jiu‐hui
Published: February 2019

Objective

The treatment of missed Monteggia fracture remains a challenge, despite the various surgical methods described. The purpose of this study was to explore a new surgical technique utilizing external fixator‐assisted ulnar osteotomy and to assess the surgical results in a case series.

Methods

Thirteen patients with missed Monteggia fractures were treated at our institution using this new surgical technique from August 2012 to January 2016. Our series included 11 boys and 2 girls. The left elbow was involved in 6 patients and the right elbow was involved in 7 patients. According to the Bado classification, 10 fractures were classified as Bado type I with anterior radial head dislocation and 3 were classified as Bado type III with anterolateral dislocation. The average age at the time of surgery was 5 years 8 months (range, 2 years 2 months–10 years). The mean trauma‐to‐surgery interval was 12 months (range, 2–36 months). All patients underwent ulnar osteotomy with angulation and lengthening using a temporary external fixator, plate fixation of the osteotomy, and open reduction of the radial head dislocation without annular ligament reconstruction.

Results

The average follow‐up was 27 months (range, 16–44 months). The average operation time was 175 min (range, 140–215 min). The average length of distraction was 0.7 cm (range, 0.5–1.2 cm) and the average angulation was 28° (range, 20°–30°) at the ulnar osteotomy site intraoperatively. The elbow performance score (Kim's) was excellent in 10 cases and good in 3 cases. No neurovascular complications, compartment syndrome or implant breakage occurred. No pain in the distal radioulnar joint or limited range of motion of the wrist occurred in any patient. The radial head remained reduced in all patients with no subluxation or redislocation. However, delayed ulnar union occurred in 3 cases, all of which were successfully treated with plaster cast immobilization within approximately 6 months postoperatively. One patient presented with cubitus valgus postoperatively with a carrying angle of 30°, which was 10° greater than the contralateral carrying angle.

Conclusions

External fixator‐assisted ulnar osteotomy offers substantial flexibility for achieving the optimal positioning of the transected ulna to reduce the radial head prior to the final ulnar osteotomy fixation with a plate, thereby facilitating an effective operative performance. Our procedure is a safe and effective method to treat missed pediatric Monteggia fractures.
De novo leukocyte chemotactic factor 2 amyloidosis in a pediatric renal allograft, 15 years post‐transplant
Authors: Shamir Eliah R. , Lee Marsha M. , Walavalkar Vighnesh
Published: February 2019

Leukocyte chemotactic factor 2 amyloidosis (ALECT2) is a recently described form of systemic amyloidosis, which most commonly affects the kidney and liver. The LECT2 protein is produced during inflammatory processes, but its precise function in renal diseases in unclear. ALECT2, however, is known to be a relatively common form of renal amyloidosis, after amyloid light chain and serum amyloid A types and is most often seen in patients of Hispanic ethnicity. ALECT2 can occur de novo or as recurrent disease in kidney transplants. We present the first case, to our knowledge, of de novo ALECT2 in a pediatric kidney transplant patient, 15 years post‐transplant.
Objectivist conditions for defeat and evolutionary debunking arguments
Authors: Klenk Michael
Published: February 2019

I make a case for distinguishing clearly between subjective and objective accounts of undercutting defeat and for rejecting a hybrid view that takes both subjective and objective elements to be relevant for whether or not a belief is defeated. Moderate subjectivists claim that taking a belief to be defeated is sufficient for the belief to be defeated; subjectivist idealists add that if an idealised agent takes a belief to be defeated then the belief is defeated. Subjectivist idealism evades some of the objections levelled against moderate subjectivism but can be shown to yield inconsistent results in some cases. Both subjectivisms should be rejected. We should be objectivists regarding undercutting defeat. This requirement, however, is likely to be problematic for a popular interpretation of evolutionary debunking arguments in metaethics as it can be shown that existing objectivist accounts of defeat do not support such arguments. I end by discussing the constraints of developing such an account.
Alterations in the rainbow trout (Oncorhynchus mykiss) eggs exposed to ionizing radiation during induced androgenesis
Authors: Polonis Marcin , Jagiełło Krzysztof , Dobosz Stefan , Rożyński Rafał , Kondraciuk Paulina , Gurgul Artur , Szmatoła Tomasz , Ocalewicz Konrad
Published: February 2019

Contents

Ionizing radiation (IR) is applied to inactivate nuclear genome in the salmonid eggs to induce androgenetic development. However, it has been considered that doses of IR used to damage maternal chromosomes may also affect morphology of the eggs and decrease their developmental potential. Thus, the main goal of the present research was to assess alterations in the rainbow trout (Oncorhynchus mykiss) eggs caused by the high dose of IR administered during androgenesis. In the present research, rainbow trout eggs were irradiated with 350 Gy of X‐rays, inseminated and exposed to the high hydrostatic pressure (HHP) shock to develop as androgenetic doubled haploids (DHs). The distribution of lipid droplets in the irradiated and non‐irradiated rainbow trout eggs, survival rates and morphology of larvae from androgenetic and control groups were compared. It has been observed that non‐irradiated and irradiated eggs exhibited altered distribution of lipid droplets. Most of the eggs before IR treatment displayed rather equal distribution of the oil droplets. In turn, majority of eggs studied after irradiation had coalesced lipid droplets, a pattern found in eggs with reduced quality. Incidences of abnormally developed larvae were more frequently observed among fish that hatched from the irradiated eggs. Observed changes suggest X‐rays applied for the genetic inactivation of rainbow trout eggs may lead to decrease of their developmental competence.
Contour current imprints and contourite drifts in the Bahamian archipelago
Authors: Mulder Thierry , Ducassou Emmanuelle , Hanquiez Vincent , Principaud Mélanie , Fauquembergue Kelly , Tournadour Elsa , Chabaud Ludivine , Reijmer John , Recouvreur Audrey , Gillet Hervé , Borgomano Jean , Schmitt Anais , Moal Paul
Published: February 2019

New data collected along the slopes of Little and Great Bahama Bank and the abyssal plain of the Bahama Escarpment provides new insights about contour current‐related erosive structures and associated deposits. The Bahamian slope shows abundant evidence of bottom current activity such as furrows, comet‐like structures, sediment waves and drifts. At a seismic scale, large erosion surfaces and main periods of drift growth resulted from current acceleration related to plate tectonic processes and progressive opening and closure of gateways and long‐term palaeoclimate evolution. At present‐day, erosion features and contourite drifts are either related to relatively shallow currents (<1000 m water depth) or to deep currents (>2500 m water depth). It appears that the carbonate nature of the drifts does not impact the drift morphology at the resolution addressed in the present study. Classical drift morphologies defined in siliciclastic environments are found, such as mounded, plastered and separated drifts. In core, contourite sequences show a bi‐gradational trend that resembles classical contourite sequences in siliciclastic deposits showing a direct relationship with a change in current velocity at the sea floor. However, in a carbonate system the peak in grain size is associated with increased winnowing rather than increased sediment supply as in siliciclastic environments. In addition, the carbonate contourite sequence is usually thinner than in siliciclastics because of lower sediment supply rates. Little Bahama Bank and Great Bahama Bank contourites contain open‐ocean input and slope‐derived debris from glacial episodes. Inner platform, platform edge and open ocean pelagic input characterize the classical periplatform ooze during interglacials. In all studied examples, the drift composition depends on the sea floor topography surrounding the drift location and the type of sediment supply. Carbonate particles are derived from either the slope or the platform in slope and toe of slope drifts, very deep contourites have distant siliciclastic sources of sediment supply. The recent discovery of the importance of a large downslope gravitary system along Bahamian slopes suggests frequent interactions between downslope and along‐slope (contour currents) processes. The interlayering of mass flow deposits and contourites at a seismic scale or the presence of surface structures associated with both contour currents and mass flow processes shows that both processes act at the same location. Finally, contour currents have an important impact on the repartition of deep‐water coral mounds. Currents can actively interact with mounds as a nutrient and oxygen supplier or have a passive interaction, with mounds solely being obstacles orienting erosion and deposition.