bluuebunny/crossref_metadata_2025 huggingface dataset:
Distinct Response of Circulating microRNAs to the Treatment of Pancreatic Cancer Xenografts with FGFR and ALK Kinase Inhibitors
Authors: Peran Ivana , Vietsch Eveline E. , Yan Gai , Riegel Anna T. , Wellstein Anton
Published: March 2022

Pancreatic adenocarcinoma is typically detected at a late stage and thus shows only limited sensitivity to treatment, making it one of the deadliest malignancies. In this study, we evaluate changes in microRNA (miR) patterns in peripheral blood as a potential readout of treatment responses of pancreatic cancer to inhibitors that target tumor–stroma interactions. Mice with pancreatic cancer cell (COLO357PL) xenografts were treated with inhibitors of either fibroblast growth factor receptor kinase (FGFR; PD173074) or anaplastic lymphoma kinase receptor (ALK; TAE684). While both treatments inhibited tumor angiogenesis, signal transduction, and mitogenesis to a similar extent, they resulted in distinct changes in circulating miR signatures. Comparison of the miR pattern in the tumor versus that in circulation showed that the inhibitors can be distinguished by their differential impact on tumor-derived miRs as well as host-derived circulating miRs. Distinct signatures that include circulating miR-1 and miR-22 are associated with the efficacy of ALK and FGFR inhibition, respectively. We propose that monitoring changes in circulating miR profiles can provide an early signature of treatment response or resistance to pathway-targeted drugs, and thus provide a non-invasive measurement to rapidly assess the efficacy of candidate therapies.
Ex Vivo Generation of CAR Macrophages from Hematopoietic Stem and Progenitor Cells for Use in Cancer Therapy
Authors: Paasch Daniela , Meyer Johann , Stamopoulou Andriana , Lenz Daniela , Kuehle Johannes , Kloos Doreen , Buchegger Theresa , Holzinger Astrid , Falk Christine S. , Kloth Christina , von Kaisenberg Constantin S. , Abken Hinrich , Schambach Axel , Lachmann Nico , Morgan Michael , Moritz Thomas
Published: March 2022

Chimeric antigen receptor (CAR) T-cell therapies have shown impressive results in patients with hematological malignancies; however, little success has been achieved in the treatment of solid tumors. Recently, macrophages (MΦs) were identified as an additional candidate for the CAR approach, and initial proof of concept studies using peripheral blood-derived monocytes showed antigen-redirected activation of CAR MΦs. However, some patients may not be suitable for monocyte-apheresis, and prior cancer treatment regimens may negatively affect immune cell number and functionality. To address this problem, we here introduce primary human hematopoietic stem and progenitor cells (HSPCs) as a cell source to generate functional CAR MΦs ex vivo. Our data showed successful CAR expression in cord blood (CB)-derived HSPCs, with considerable cell expansion during differentiation to CAR MΦs. HSPC-derived MΦs showed typical MΦ morphology, phenotype, and basic anti-bacterial functionality. CAR MΦs targeting the carcinoembryonic antigen (CEA) and containing either a DAP12- or a CD3ζ-derived signaling domain showed antigen redirected activation as they secreted pro-inflammatory cytokines specifically upon contact with CEA+ target cells. In addition, CD3ζ-expressing CAR MΦs exhibited significantly enhanced phagocytosis of CEA+ HT1080 cells. Our data establish human HSPCs as a suitable cell source to generate functional CAR MΦs and further support the use of CAR MΦs in the context of solid tumor therapy.
Fetal Surgery for Gastroschisis—A Review with Emphasis on Minimally Invasive Procedures
Authors: Durmaz Lidya-Olgu , Brunner Susanne Eva , Meinzer Andreas , Krebs Thomas Franz , Bergholz Robert
Published: March 2022

(1) Background: The morbidity of gastroschisis is defined by exposure of unprotected intestines to the amniotic fluid leading to inflammatory damage and consecutive intestinal dysmotility, the viscero-abdominal disproportion which results in an abdomen too small to incorporate the herniated and often swollen intestine, and by associated pathologies, such as in complex gastroschisis. To prevent intestinal damage and to provide for growth of the abdominal cavity, fetal interventions such as amnio exchange, gastroschisis repair or covering have been evaluated in several animal models and human trials. This review aims to evaluate the reported techniques for the fetal treatment of gastroschisis by focusing on minimally invasive procedures. (2) Methods: We conducted a systematic database search, quality assessment and analyzed relevant articles which evaluate or describe surgical techniques for the prenatal surgical management of gastroschisis in animal models or human application. (3) Results: Of 96 identified reports, 42 eligible studies were included. Fetal interventions for gastroschisis in humans are only reported for EXIT procedures and amnio exchange. In animal models, particularly in the fetal sheep model, several techniques of open or minimally invasive repair of gastroschisis or covering the intestine have been described, with fetoscopic covering being the most encouraging. (4) Discussion: Although some promising minimally invasive techniques have been demonstrated in human application and animal models, most of them are still associated with relevant fetal morbidity and mortality and barely appear to be currently applicable in humans. Further research on specific procedures, instruments and materials is needed before any human application.
Liver Resection Using Saline-Linked Radiofrequency Technology in an Infant with Congenital Hepatoblastoma
Authors: Torino Giovanni , Ilari Michele , Bindi Edoardo , Mariscoli Francesca , Cobellis Giovanni
Published: March 2022

We herein report a case of giant congenital hepatoblastoma in a 3-month-old male treated with neoadjuvant chemotherapy and hepatic resection. After considerable reduction of the tumor with chemotherapy, a right bloodless hemihepatectomy using saline-linked radiofrequency technology (SLRT) and without clamping of the hepatic pedicle was performed. Intraoperative blood loss was minimal, and consequently, no blood transfusions were required. The surgery lasted 140 min, and SLRT was used for a total of 60 min. No complications were observed during or after the surgery. In conclusion, congenital hepatoblastoma is a very rare cancer for which surgery is an essential therapeutic step, and in our presented case, we showed that SLRT allowed for a safe and effective bloodless liver resection.
Incidental Finding in Pre-Orthodontic Treatment Radiographs of an Aural Foreign Body: A Case Report
Authors: Maspero Cinzia , Abate Andrea , Inchingolo Francesco , Dolci Claudia , Cagetti Maria Grazia , Tartaglia Gianluca Martino
Published: March 2022

The presence of foreign bodies in the external auditory canal of young patients may cause, if left untreated, severe permanent damage to the adjacent anatomical structures, and infections. A 10-year-old patient with an intellectual disability underwent orthodontic evaluation. An aural radiopaque finding was visible in the lateral cephalogram and in the orthopantomography. The patient’s mother reported that her son never showed any ear discomfort, except for a mild hearing impairment that was never investigated. The patient was referred to an ear, nose and throat (ENT) specialist that removed the foreign body located in the left external auditory meatus. The careful evaluation of dental radiographs, including pre-orthodontic and interim orthodontic radiographs, may help to identify silent incidental findings that may otherwise lead to severe complications if left untreated.
Impacts of Multiple Hurricanes and Tropical Storms on Watershed Hydrological Processes in the Florida Panhandle
Authors: Ouyang Ying , Grace Johnny M. , Parajuli Prem B. , Caldwell Peter V.
Published: March 2022

Hurricanes and tropical storms (TS) are infrequent but disastrous events to human lives, social activities, and terrestrial ecosystems in coastal regions. Using the Environmental Protection Agency (US-EPA)’s Hydrologic and Water Quality System (HAWQS) model, principal component analysis (PCA), and principal factor analysis (PFA), we estimated impacts of multiple hurricanes and TS on hydrological processes in agricultural and forested watersheds. Five hurricanes and four TS that passed near or through the Apalachicola–Chattahoochee–Flint River basin (ACFRB) of the Florida panhandle from 1966 to 2018 were selected to estimate their impacts on rainfall, potential evapotranspiration (PET), evapotranspiration (ET), soil water percolation, surface runoff, stream discharge, groundwater recharge, and water yield (WYLD). Simulations showed that the category of hurricanes was not highly related to the amounts of rainfall, runoff, discharge, and WYLD. Based on PCA and PFA, PET and ET were highly and negatively, rainfall and discharge were highly and positively, and percolation, runoff, groundwater recharge and WYLD were moderately and positively affected by the hurricanes and TS at the ACFRB in the recent 50 years. This study provides water resource managers with critical insights into how multiple hurricanes and TS affected hydrological processes in agricultural and forested watersheds of the coastal region.
Nondestructive Evaluation of Thermal Barrier Coatings Thickness Using Terahertz Technique Combined with PCA–GA–ELM Algorithm
Authors: Yuan Baohan , Wang Weize , Ye Dongdong , Zhang Zhenghao , Fang Huanjie , Yang Ting , Wang Yihao , Zhong Shuncong
Published: March 2022

Thermal barrier coatings (TBCs) are usually used in high temperature and harsh environment, resulting in thinning or even spalling off. Hence, it is vital to detect the thickness of the TBCs. In this study, a hybrid machine learning model combined with terahertz time-domain spectroscopy technology was designed to predict the thickness of TBCs. The terahertz signals were obtained from the samples prepared in laboratory and actual turbine blade. The principal component analysis (PCA) method was used to decrease the data dimensions. Finally, an extreme learning machine (ELM) was proposed to establish the thickness of TBCs prediction model. Genetic algorithm (GA) was selected to optimize the model to make it more accurate. The results showed that the root correlation coefficient (R2) exceeded 0.97 and the errors (root mean square error and mean absolute percentage error) were less than 2.57. This study proposes that terahertz time-domain technology combined with PCA–GA–ELM model is accurate and feasible for evaluating the thickness of the TBCs.
Computation of Nonlinear Load Harmonic Currents in the Presence of External Distortions
Authors: Skamyin Aleksandr , Belsky Aleksey , Dobush Vasiliy , Gurevich Ilya
Published: March 2022

This paper deals with the issues of computation of the nonlinear load harmonic currents in the presence of external distortions based on the real measurements with help of passive harmonic. Such values are necessary when modeling an equivalent nonlinear load as current sources in the presence of external distortions. The passive filter allows to separate external and internal distortions, which is necessary when determining the harmonic currents of a single consumer. The influence of various parameters on the computation of harmonic currents of a nonlinear load, such as the parameters of a passive harmonic filter, the impedance of the power supply transformer, the load parameters of the consumer, taking into account the harmonic generation from the grid side, and from the consumer side, is considered. It is shown that an external source of distortion has practically no effect on the error in estimating the harmonic current magnitudes of a nonlinear load. The obtained simulation results were confirmed experimentally on a laboratory test bench. Recommendations for the selection of passive harmonic filter parameters have been developed to minimize the error in determining the harmonic current magnitudes of a nonlinear load.
Primary Effusion Lymphoma: A Timely Review on the Association with HIV, HHV8, and EBV
Authors: Liu Chih-Yi , Chen Bo-Jung , Chuang Shih-Sung
Published: March 2022

Primary effusion lymphoma (PEL) is defined by the WHO classification as a large B-cell neoplasm without detectable tumor masses. It is universally associated with HHV8, with most cases occurring in the setting of immunodeficiency such as HIV infection, and a poor prognosis. Morphologically, the neoplastic cells range from immunoblastic, plasmablastic, to anaplastic; and phenotypically, most cases express plasma cell but not B-cell markers, i.e., plasmablastic. During the past decade, primary HHV8-negative effusion lymphoma has been reported. Such cases were considered in the WHO classification scheme as effusion-based lymphoma. We performed a systemic review of 167 HHV8-negative effusion lymphomas from the literature and found that only 42% were associated with a fluid overload state, and with low rates of HIV (6%) or EBV (21%) infection. Furthermore, most patients are old (or immunosenescent) with underlying medical conditions/comorbidities, most neoplasms are of B-cell phenotype, and the outcome is more favorable than that of HHV8-positive PEL. These distinctive findings supported our prior proposal of designating these HHV8-negative cases as type II PEL, in contrast to the classic or type I PEL as defined by the WHO. Furthermore, we propose an algorithmic approach for the diagnosis of PEL and its mimickers.
A Smart Tourism Case Study: Classification of Accommodation Using Machine Learning Models Based on Accommodation Characteristics and Online Guest Reviews
Authors: Čumlievski Nola , Brkić Bakarić Marija , Matetić Maja
Published: March 2022

This paper deals with the analysis of data retrieved from a web page for booking accommodation. The main idea of the research is to analyze the relationship between accommodation factors and customer reviews in order to determine the factors that have the greatest influence on customer reviews. Machine learning methods are applied to the collected data and models that can predict the review category for those accommodations that are not evaluated by users are trained. The relationship between certain accommodation factors and classification accuracy of the models is examined in order to get detailed insight into the data used for model training, as well as to make the models more interpretable. The classification accuracy of each model is tested and the precision and recall of the models are examined and compared.
Accounting and Market Risk Measures of Polish Energy Companies
Authors: Rutkowska-Ziarko Anna , Markowski Lesław
Published: March 2022

Companies in the energy sector, due to their important role in the economy and the specificity of energy sources, are exposed to many types of risk, ranging from the risk associated with the company’s operations and the global economic and political situation in the world. Energy companies are usually large capital companies whose shares are listed on the stock market. The mentioned risk factors may shape the risk level of these companies. The study aims to examine the relationship between market and accounting risk measures for Polish energy companies listed on the Warsaw Stock Exchange. This paper uses market and accounting betas in the conventional and downside approach. In addition to market measures of total risk, it also examines the variability of ROA for energy companies. The study of the relationship between market risk measures and accounting risk measures was based on Pearson’s correlation coefficient, standard linear regression, and quantile regression. The relationship between market and accounting measures of total and systematic risk was identified. Moreover, quantile regressions revealed that the slope for accounting variables varies across the quantiles. Our research shows that for energy companies not listed on the capital markets, for which no market risk measures can be derived, accounting betas and downside accounting can be useful tools in risk analysis. The contribution of the article to the risk analysis of energy companies is the use of unpopular accounting beta factors and a new modification of these coefficients for downside risk.
Analysis of a Wind-Driven Air Compression System Utilising Underwater Compressed Air Energy Storage
Authors: Swinfen-Styles Lawrie , Garvey Seamus D. , Giddings Donald , Cárdenas Bruno , Rouse James P.
Published: March 2022

The increasing push for renewable penetration into electricity grids will inevitably lead to an increased requirement for grid-scale energy storage at multiple time scales. It will, necessarily, lead to a higher proportion of the total energy consumed having been passed through storage. Offshore wind is a key technology for renewable penetration, and the co-location of energy storage with this wind power provides significant benefits. A novel generation-integrated energy storage system is described here in the form of a wind-driven air compressor feeding underwater compressed air energy storage. A direct drive compressor would require very high intake swept volumes. To overcome this difficulty, some prior compression is introduced. This paper discusses the constituent technologies for this concept, as well as the various configurations that it might take and the logic behind operating it. Special consideration has been given to the differences resulting from utilising a near-isothermal wind-driven compressor versus a near-adiabatic one. Multiple iterations of the system have been simulated. This has been done using a price-matching algorithm to optimise the system operation and using volumetric air flow rates to calculate exergy flow. Simulated operation has been performed for a year of real wind and electricity price data. This work has been performed in order to clarify the relationships between several key parameters in the system, including pressure and work ratios, volumetric flowrates, storage costs and profit rates. An additional objective of this paper was to determine whether the system has the potential for economic viability in some future energy grid, especially when compared with alternative wind and energy storage solutions. The results of the simulation indicated that, with proper sizing, the system might perform competitively with these alternatives. Maximum one-year return on investment values of 9.8% for the isothermal case and 13% for the adiabatic case were found. These maxima were reached with ~15–20 h of output storage. In all cases, it was found that maximising the power of the wind-driven compressor compared with the initial compressor was favourable.
Impact of Local Emergency Demand Response Programs on the Operation of Electricity and Gas Systems
Authors: Davari Mohammad Mehdi , Ameli Hossein , Ameli Mohammad Taghi , Strbac Goran
Published: March 2022

With increasing attention to climate change, the penetration level of renewable energy sources (RES) in the electricity network is increasing. Due to the intermittency of RES, gas-fired power plants could play a significant role in backing up the RES in order to maintain the supply–demand balance. As a result, the interaction between gas and power networks are significantly increasing. On the other hand, due to the increase in peak demand (e.g., electrification of heat), network operators are willing to execute demand response programs (DRPs) to improve congestion management and reduce costs. In this context, modeling and optimal implementation of DRPs in proportion to the demand is one of the main issues for gas and power network operators. In this paper, an emergency demand response program (EDRP) is implemented locally to reduce the congestion of transmission lines and gas pipelines more efficiently. Additionally, the effects of optimal implementation of local emergency demand response program (LEDRP) in gas and power networks using linear and non-linear economic models (power, exponential and logarithmic) for EDRP in terms of cost and line congestion and risk of unserved demand are investigated. The most reliable demand response model is the approach that has the least difference between the estimated demand and the actual demand. Furthermore, the role of the LEDRP in the case of hydrogen injection instead of natural gas in the gas infrastructure is investigated. The optimal incentives for each bus or node are determined based on the power transfer distribution factor, gas transfer distribution factor, available electricity or gas transmission capability, and combination of unit commitment with the LEDRP in the integrated operation of these networks. According to the results, implementing the LEDRP in gas and power networks reduces the total operation cost up to 11% and could facilitate hydrogen injection to the network. The proposed hybrid model is implemented on a 24-bus IEEE electricity network and a 15-bus gas network to quantify the role and value of different LEDRP models.
Correction: Alasali et al. Forecasting and Modelling the Uncertainty of Low Voltage Network Demand and the Effect of Renewable Energy Sources. Energies 2021, 14, 2151
Authors: Alasali Feras , Foudeh Husam , Ali Esraa Mousa , Nusair Khaled , Holderbaum William
Published: March 2022

Error in Figures [...]
Genetic and Epigenetic Inheritance at Telomeres
Authors: Lister-Shimauchi Evan H. , McCarthy Benjamin , Lippincott Michael , Ahmed Shawn
Published: March 2022

Transgenerational inheritance can occur at telomeres in distinct contexts. Deficiency for telomerase or telomere-binding proteins in germ cells can result in shortened or lengthened chromosome termini that are transmitted to progeny. In human families, altered telomere lengths can result in stem cell dysfunction or tumor development. Genetic inheritance of altered telomeres as well as mutations that alter telomeres can result in progressive telomere length changes over multiple generations. Telomeres of yeast can modulate the epigenetic state of subtelomeric genes in a manner that is mitotically heritable, and the effects of telomeres on subtelomeric gene expression may be relevant to senescence or other human adult-onset disorders. Recently, two novel epigenetic states were shown to occur at C. elegans telomeres, where very low or high levels of telomeric protein foci can be inherited for multiple generations through a process that is regulated by histone methylation.Together, these observations illustrate that information relevant to telomere biology can be inherited via genetic and epigenetic mechanisms, although the broad impact of epigenetic inheritance to human biology remains unclear.
Health Benefits of Postbiotics Produced by E. coli Nissle 1917 in Functional Yogurt Enriched with Cape Gooseberry (Physalis peruviana L.)
Authors: Darwish Mohamed Samir , Qiu Longxin , Taher Mohamed A. , Zaki Ahmed A. , Abou-Zeid Noha A. , Dawood Dawood H. , Shalabi Ola M. A. K. , Khojah Ebtihal , Elawady Asmaa A.
Published: March 2022

Changes in the activities of antimicrobial, antitumor, and antioxidant properties of postbiotics (YCG) are related to changes in the composition of phenolic compounds. Antimicrobial activity was found to be highest in postbiotic (YCG-7) against P. aeruginosa, S. aureus, and E. faecalis with an MIC of 3.1 µg/mL. YCG-7 revealed the most cytotoxicity against LS-174T and PC-3 cell lines with an IC50 of 5.78 and 6.56 µg/mL, respectively. YCG-7 was far more effective for scavenging free radicals in the NO• and DPPH assays with a scavenging activity of 70.73% and 85.6%, respectively. YCG-7’s total phenolic acid content is up to eightfold higher compared with control. Escherichia coli Nissle 1917 retained high viable counts during refrigerated storage, particularly in YCG (>108 cells g−1) revealing a potential prebiotic activity of Cape gooseberry juice. EcN affected the phenolic profile of the YCG. Pyrogallol, p-coumaric acid, ellagic acid, 4-hydroxybenzoic acid, salicylic acid, gallic acid, vanillic acid, o-coumaric acid, caffeic acid, catechol, syringic acid, and rutin were the predominant phenolic compounds in YCG-7 or YCG-15. Chlorogenic, rosmarinic, cinnamic acid, naringin, and kaempferol were degraded by EcN in YCG-7 and YCG-15. The YCG had significantly higher sensory scores for appearance, smoothness, sourness, mouthfeel, and overall acceptance. These results provide the basis to target the functional benefits of YCG for further human health applications.
The Time Machine in Columnar NoSQL Databases: The Case of Apache HBase
Authors: Tsai Chia-Ping , Chang Che-Wei , Hsiao Hung-Chang , Shen Haiying
Published: March 2022

Not Only SQL (NoSQL) is a critical technology that is scalable and provides flexible schemas, thereby complementing existing relational database technologies. Although NoSQL is flourishing, present solutions lack the features required by enterprises for critical missions. In this paper, we explore solutions to the data recovery issue in NoSQL. Data recovery for any database table entails restoring the table to a prior state or replaying (insert/update) operations over the table given a time period in the past. Recovery of NoSQL database tables enables applications such as failure recovery, analysis for historical data, debugging, and auditing. Particularly, our study focuses on columnar NoSQL databases. We propose and evaluate two solutions to address the data recovery problem in columnar NoSQL and implement our solutions based on Apache HBase, a popular NoSQL database in the Hadoop ecosystem widely adopted across industries. Our implementations are extensively benchmarked with an industrial NoSQL benchmark under real environments.
Nature-Inspired Antimicrobial Surfaces and Their Potential Applications in Food Industries
Authors: Soni Aswathi , Brightwell Gale
Published: March 2022

Antimicrobial resistance (AMR) is a growing global concern and has called for the integration of different areas of expertise for designing robust solutions. One such approach is the development of antimicrobial surfaces to combat the emerging resistance in microbes against drugs and disinfectants. This review is a compressive summary of the work done in the field of material science, chemistry, and microbiology in the development of antimicrobial materials and surfaces that are inspired by examples in nature. The focus includes examples of natural antimicrobial surfaces, such as cicada wings or nanopillars, dragonfly wings, shrimp shells, taro leaves, lotus leaves, sharkskin, gecko skin, and butterfly wings, along with their mechanism of action. Techniques, compositions, and combinations that have been developed to synthetically mimic these surfaces against bacterial/viral and fungal growth in food-processing areas have also been discussed. The applications of synthetic mimics of natural antimicrobial surfaces in food-processing environments is still a naïve area of research. However, this review highlights the potential applications of natural antimicrobial surfaces in the food-processing environment as well as outlines the challenges that need mitigations.
Examining the Association between Mitochondrial Genome Variation and Coronary Artery Disease
Authors: Vilne Baiba , Sawant Aniket , Rudaka Irina
Published: March 2022

Large-scale genome-wide association studies have identified hundreds of single-nucleotide variants (SNVs) significantly associated with coronary artery disease (CAD). However, collectively, these explain <20% of the heritability. Hypothesis: Here, we hypothesize that mitochondrial (MT)-SNVs might present one potential source of this “missing heritability”. Methods: We analyzed 265 MT-SNVs in ~500,000 UK Biobank individuals, exploring two different CAD definitions: a more stringent (myocardial infarction and/or revascularization; HARD = 20,405), and a more inclusive (angina and chronic ischemic heart disease; SOFT = 34,782). Results: In HARD cases, the most significant (p < 0.05) associations were for m.295C>T (control region) and m.12612A>G (ND5), found more frequently in cases (OR = 1.05), potentially related to reduced cardiorespiratory fitness in response to exercise, as well as for m.12372G>A (ND5) and m.11467A>G (ND4), present more frequently in controls (OR = 0.97), previously associated with lower ROS production rate. In SOFT cases, four MT-SNVs survived multiple testing corrections (at FDR < 5%), all potentially conferring increased CAD risk. Of those, m.11251A>G (ND4) and m.15452C>A (CYB) have previously shown significant associations with body height. In line with this, we observed that CAD cases were slightly less physically active, and their average body height was ~2.00 cm lower compared to controls; both traits are known to be related to increased CAD risk. Gene-based tests identified CO2 associated with HARD/SOFT CAD, whereas ND3 and CYB associated with SOFT cases (p < 0.05), dysfunction of which has been related to MT oxidative stress, obesity/T2D (CO2), BMI (ND3), and angina/exercise intolerance (CYB). Finally, we observed that macro-haplogroup I was significantly (p < 0.05) more frequent in HARD cases vs. controls (3.35% vs. 3.08%), potentially associated with response to exercise. Conclusions: We found only spurious associations between MT genome variation and HARD/SOFT CAD and conclude that more MT-SNV data in even larger study cohorts may be needed to conclusively determine the role of MT DNA in CAD.
RNA-Seq Reveals Differentially Expressed Genes Associated with High Fiber Quality in Abaca (Musa textilis Nee)
Authors: Ereful Nelzo C. , Lalusin Antonio G. , Laurena Antonio C.
Published: March 2022

Despite the importance of and current demand for abaca (Musa textilis Nee) fiber, there has been limited study that capitalizes on RNA-seq to identify candidate genes associated with high fiber quality and bunchy top virus (AbBTV) resistance. Three varieties (Abuab, Inosa, and Tangongon), one wild banana variety (Musa balbisiana Colla) Pacol, and two developed backcrosses (Abuab × Pacol BC2 and BC3) were grown at the Institute of Plant Breeding (IPB), Laguna, Philippines. The pseudostems of 3-month-old suckers of each genotype were sampled for RNA-seq. Datasets were analyzed for differential expression (DE) implementing various model frameworks, including pairwise, genotypic and non-DE models. Results indicate that Abuab and BC3 induce the highest proportion (70%) of abaca-specific genes. Gene ontology (GO) enrichment analysis showed several genes associated with cellulose synthase activity, callose synthase, ß-glucosidase activity, glucan biosynthetic process, etc. KEGG pathway analysis showed several genes encoding for enzymes involved in the lignin biosynthetic pathway. Analysis using genotypic DE (GDE) between abaca bunchy top virus (AbBTV)-resistant and -susceptible groups revealed genes such as pathogenesis-related protein and NBS-LRR. As the genotypes were not infected with the pathogen, these genes are yet to be confirmed for their roles in disease resistance and are an interesting subject for further investigation.
Interaction between Work and Metabolic Syndrome: A Population-Based Cross-Sectional Study
Authors: Santana Amália Ivine Costa , das Merces Magno Conceição , de Souza Marcio Costa , de Carvalho Lima Bruno Gil , Galdino Maria José Quina , de Carvalho Félix Nuno Damácio , Magalhães Lucelia Batista Neves Cunha , Coelho Julita Maria Freitas , Barbosa Paulo José Bastos , Dias Gomes Érica Velasco , Pimentel Rodrigo Fernandes Weyll , de Sousa Anderson Reis , de Oliveira Márcia Aparecida Ferreira , de Queiroz Aline Macêdo , Florencio Raíssa Millena Silva , Cavalcante Neto Jorge Lopes , Gomes Antonio Marcos Tosoli , Souza Santos Thadeu Borges , Vieira Silvana Lima , de Sousa Danilo Guimarães , da Silva Thiengo de Andrade Priscila Cristina , de Negreiros Nogueira Maduro Isolda Prado , Fernandes Sandra Lúcia , Damasceno Kairo Silvestre Meneses , da Silva Dandara Almeida Reis , D’Oliveira Júnior Argemiro
Published: March 2022

Metabolic syndrome (MS) is a clinical condition and a relevant risk factor in the development of cardiovascular diseases; it occurs as a result of lifestyle factors, e.g., work. The aim of this research was to estimate the interaction between work and MS among primary health care (PHC) nursing professionals in the state of Bahia, Brazil. A sectional multicentered study carried out in 43 municipalities in Bahia, whose study population consisted of nursing professionals. The exposure variables were occupation, professional exhaustion, and working time, and the outcome variable was MS. Interaction measures based on the additivity criteria were verified by calculating the excess risks due to the interactions and according to the proportion of cases attributed to the interactions and the synergy index. The global MS prevalence is 24.4%. There was a greater magnitude in the exposure group regarding the three investigated factors (average level occupation, professional exhaustion, and working time in PHC for more than 5 years), reaching an occurrence of 44.9% when compared to the prevalence of 13.1% in the non-exposure group (academic education, without professional burnout, and working time in PHC for up to 5 years). The study’s findings showed a synergistic interaction of work aspects for MS occurrence among PHC nursing professionals.
The Role of Competition Area and Training Type on Physiological Responses and Perceived Exertion in Female Judo Athletes
Authors: Ouergui Ibrahim , Delleli Slaheddine , Chtourou Hamdi , Formenti Damiano , Bouhlel Ezdine , Ardigò Luca Paolo , Franchini Emerson
Published: March 2022

This study investigated the combined effects of competition area (4 × 4, 6 × 6, and 8 × 8 m) and judo-specific training type (tachi-waza, ne-waza, and free randori) on physiological responses and perceived exertion in female judo athletes. In a within-subject design, 12 female subelite and elite athletes who competed at regional or national levels with a mean training background of 8.4 ± 0.5 years performed the experimental conditions (i.e., combats (viz., matches) featuring different area/training type combinations) in random order. The following measurements at different time points were chosen: blood lactate before and after each match; heart rate before, mean, and peak for each match; and rating of perceived exertion immediately after each match. Two-factor analysis of variance was used to compare between conditions, while Bonferroni post hoc test and magnitude of difference were used to measure significance. There was no main effect of training type or area size on lactate before each match, heart rate (HR) before each match, HR mean during each match, and rating of perceived exertion. Main effects of training type and area size were found for lactate after each event, with the values being greater in free randori compared to tachi-waza and ne-waza and in 4 × 4 m compared to 6 × 6 and 8 × 8 m area. Main effects of training type and area size were also found in peak heart rate, with lower values in ne-waza compared to free randori and tachi-waza and in 8 × 8 m compared to 4 × 4 m area. The results demonstrate that varying training modality and area size may alter physiological responses during female judo combats by putting stress on the cardiovascular system and increasing anaerobic glycolysis solicitation.
“Precious Time Together Was Taken Away”: Impact of COVID-19 Restrictive Measures on Social Needs and Loneliness from the Perspective of Residents of Nursing Homes, Close Relatives, and Volunteers
Authors: Noten Suzie , Stoop Annerieke , De Witte Jasper , Landeweer Elleke , Vinckers Floor , Hovenga Nina , van Boekel Leonieke C. , Luijkx Katrien G.
Published: March 2022

During the COVID-19 outbreak in March 2020, restrictive measures (e.g., prohibiting physical visits and group activities) were introduced in nursing homes to protect older residents. Although the importance of social contacts and social activities to fulfill social needs and avoid loneliness is known, these were challenged during the pandemic. This qualitative study specifically focused on how residents, close relatives, and volunteers in nursing homes experienced the restrictive measures in retrospect and gained insights into the impact of the restrictive measures on social needs and loneliness, and the lessons that could be learned. Thirty semi-structured, face-to-face interviews with residents and close relatives, and one online focus group with ten volunteers, were conducted. Recruitment took place at psychogeriatric and somatic units in the Northern, Eastern and Southern regions of the Netherlands and Flanders, Belgium. The interviews and focus group were transcribed verbatim, and an open, inductive approach was used for analysis. Alternative ways of social contact could not fully compensate for physical visits. Generally, participants reported that it was a difficult time, indicated by feelings of loneliness, fear, sadness, and powerlessness. A great diversity in loneliness was reported. The most important reasons for feeling lonely were missing close social contacts and social activities. The diversity in the impact of restrictive measures depended on, e.g., social needs, coping strategies, and character. Restrictive COVID-19 measures in nursing homes resulted in negative emotions and unmet social needs of residents, close relatives, and volunteers. During future outbreaks of the COVID-19 virus or another virus or bacterium, for which restrictive measures may be needed, nursing homes should actively involve residents, close relatives, and volunteers to balance safety, self-determination, and well-being.
Awareness and Perceptions among Members of a Japanese Cancer Patient Advocacy Group Concerning the Financial Relationships between the Pharmaceutical Industry and Physicians
Authors: Murayama Anju , Senoo Yuki , Harada Kayo , Kotera Yasuhiro , Saito Hiroaki , Sawano Toyoaki , Suzuki Yosuke , Tanimoto Tetsuya , Ozaki Akihiko
Published: March 2022

Objectives: Awareness and perceptions of financial conflicts of interest (FCOI) between pharmaceutical companies (Pharma) and healthcare domains remain unclear in Japanese cancer patient communities. This study aimed to assess awareness (RQ1), the influence of FCOI on physician trustworthiness (RQ2), and their perception (RQ3) among the Japanese cancer patient advocacy group members. Methods: A cross-sectional study using a self-administered survey was conducted with a Japanese cancer patient advocacy group between January and February 2019. The main outcome measures included awareness and perceptions of physician–Pharma interactions, their impact on physician trustworthiness, and attitudes towards FCOI among medical and other professions. Furthermore, we performed thematic analyses on the comments which responders provided in the surveys. Results: Among the 524 contacted members, 96 (18.3%) completed the questionnaire, including 69 (77.5%) cancer patients. In RQ1, most of the respondents were aware of physician–Pharma interactions, although the extent differed based on the nature of the interaction. Furthermore, the respondents mainly considered these interactions influential on clinical practice (RQ2) and agreed to the need for further regulation of physician–Pharma interactions (QR3). In qualitative analyses (n = 56), we identified the 4 following themes: perception towards the FCOI (Theme 1), concerns about the respondent’s treatment (Theme 2), reason of physician–Pharma interactions (Theme 3), and possible solutions from the patient perspective (Theme 4). Conclusions: Most respondents were generally aware of physician–Pharma-associated FCOI and perceived them negatively. Additionally, participants appeared supportive of further FCOI regulation to protect patient-centred care. Abbreviations: FCOI—financial conflicts of interest; United States—US; Pharma—pharmaceutical companies; RQ—research question.
Comparative Nanopore Sequencing-Based Evaluation of the Midgut Microbiota of the Summer Chafer (Amphimallon solstitiale L.) Associated with Possible Resistance to Entomopathogenic Nematodes
Authors: Sajnaga Ewa , Skowronek Marcin , Kalwasińska Agnieszka , Kazimierczak Waldemar , Lis Magdalena , Jach Monika Elżbieta , Wiater Adrian
Published: March 2022

Root-feeding Amphimallon solstitiale larvae and certain other scarab beetles are the main soil-dwelling pests found in Europe, while entomopathogenic nematodes (EPN) have been used as a biocontrol agent against these species. Our study provides the first detailed characterization of the bacterial community of the midgut in wild A. solstitiale larvae, based on the nanopore sequencing of the 16S rRNA gene. In the whole dataset, we detected 2586 different genera and 11,641 species, with only 83 diverse bacterial genera shared by all studied individuals, which may represent members of the core midgut microbiota of A. solstitiale larvae. Subsequently, we compared the midgut microbiota of EPN-resistant and T0 (prior to EPN exposure) individuals, hypothesizing that resistance to this parasitic infection may be linked to the altered gut community. Compared to the control, the resistant insect microbiota demonstrated lower Shannon and Evenness indices and significant differences in the community structure. Our studies confirmed that the gut microbiota alternation is associated with resistant insects; however, there are many processes involved that can affect the bacterial community. Further research on the role of gut microbiota in insect-parasitic nematode interaction may ultimately lead to the improvement of biological control strategies in insect pest management.
The Mediating Effects of Work–Life Balance (WLB) and Ease of Using WLB Programs in the Relationship between WLB Organizational Culture and Turnover Intention
Authors: Yu Han-Sun , Lee Eun-Jun , Na Tae-Kyun
Published: March 2022

Work–life balance (WLB) is an important concern for all workers irrespective of their age, sex, education level, family structure, or occupation. This study analyzes WLB’s mediating effects and the ease of using WLB programs in the relationship between WLB organizational culture of hotels and turnover intention of its culinary staff. We conducted a survey featuring 320 culinary staff members at hotels in Incheon from 10 to 30 August 2020 and performed statistical analysis using 290 responses. We find that the company’s willingness for WLB, empathetic communication with colleagues, material support of colleagues for WLB, and the ease of using WLB programs in organizational culture had a positive impact on WLB. The company’s willingness for WLB, boss’s consideration for WLB, empathetic communication with colleagues, and material support of colleagues for WLB in organizational culture had a negative impact on turnover intention. The ease of using WLB programs had no indirect effect on the relationship between organizational culture and turnover intention. However, WLB had an indirect effect on the relationship between the four components except for the boss’s consideration for WLB and turnover intention. Hotel management should create an organizational culture that supports the WLB of culinary staff.
Cost-Benefit Analysis of an Intervention in Divorced Parents: Implications for Society, Public Administrations and Family Visitation Centers
Authors: Alcaniz Leire , Martínez-Pampliega Ana , Herrero Marta
Published: March 2022

Families going through conflictive divorce processes are at increased risk of developing mental health problems. The Egokitzen program is a group intervention for parents who have undergone a divorce process, funded by the public administration. Budgetary constraints cause funding institutions to be interested in the effectiveness and economic efficiency of these programs. Therefore, the objective of this research is to carry out an efficiency analysis of the Egokitzen program, implemented by family visitation centers in Spain, through a cost-benefit analysis, to determine whether the positive impact on symptomatology (measured using CBCL and SCL-90 instruments) is translated into a positive economic impact for society. A sample of 382 parents participated. Costs will be first identified and valued; secondly, benefits achieved with the program will be identified through a prevalence analysis and, finally, the cost-benefit comparative analysis will be carried out. Additionally, a sensitivity analysis will be performed. The results obtained in the analysis indicate that for every euro spent on this program, the public administration and society save 3.10 euros in future interventions through medical costs and productivity losses. The study has practical implications for public administration, organizations, and the family visitation centers that implement the program.
Association between Residential Greenness and Incidence of Parkinson’s Disease: A Population-Based Cohort Study in South Korea
Authors: Jung Jiyun , Park Jae Yoon , Myung Woojae , Lee Jun-Young , Ko Hyunwoong , Lee Hyewon
Published: March 2022

It is widely known that exposure to residential greenness is beneficial for health. However, few studies have analyzed the association between greenery and Parkinson’s disease (PD). We selected 313,355 participants who matched the inclusion criteria from the National Health Insurance Service-National Sample Cohort, followed up from 2007 to 2015. Residential greenness, represented by the normalized difference vegetation index (NDVI), was obtained from satellite measurements. We estimated hazard ratios of PD associated with a 0.1-unit increase in long-term greenness exposure at the district level for the previous 1 year of each year until a censoring/event occurred, using time-varying Cox proportional hazard models, adjusted for individual- and area-level characteristics. During the 2,745,389 person-years of follow-up, 2621(0.8%) participants developed PD. Exposure to higher levels of residential greenness was found to be associated with a decreased risk of PD incidence (21% per 0.1-unit increase, 95% confidence interval (CI): 0.74–0.84). In subgroup analyses, stronger protective effects were observed in participants aged over 50 years, females, overweight/obese participants, non-urban residents, non-smokers, alcoholics, and those with comorbidities. Long-term exposure to greenness was beneficial to incident PD, and our findings could aid in the development of public-health strategies.