bluuebunny/crossref_metadata_2025 huggingface dataset:
What Drives Caterpillar Guilds on a Tree: Enemy Pressure, Leaf or Tree Growth, Genetic Traits, or Phylogenetic Neighbourhood?
Authors: Molleman Freerk , Walczak Urszula , Melosik Iwona , Baraniak Edward , Piosik Łukasz , Prinzing Andreas
Published: April 2022

Communities of herbivorous insects on individual host trees may be driven by processes ranging from ongoing development via recent microevolution to ancient phylogeny, but the relative importance of these processes and whether they operate via trophic interactions or herbivore movement remains unknown. We determined the leaf phenology, trunk diameter, genotype, and neighbourhood of sessile oak trees (Quercus petraea), and sampled their caterpillar communities. We found that leaf development across a time period of days related to free-living caterpillars, which disappeared with leaf age. Tree growth across decades is related to increased parasitism rate and diversity of herbivores. The microevolution of oak trees across millennia is related to the abundance of leaf-mining casebearers, which is higher on more homozygous oaks. However, oak genome size was not important for any guild. In contrast to most previous studies, the phylogenetic distance of oaks from their neighbours measured in millions of years was associated with higher abundances of entire caterpillar guilds. Furthermore, on trees surrounded by only distantly related tree species, parasitism tended to be lower. Lower parasitism, in turn, was associated with higher abundances of codominant caterpillar species. Neighbourhoods and traits of trees were also related to community composition and diversity, but not to the average wingspans or specialization of species, consistent with the assembly of herbivore communities being driven by leaf traits and parasitism pressure on trees rather than by insect movement among trees. However, movement in rarer species may be responsible for concentration effects in more phylogenetically distant neighbourhoods. Overall, we suggest that the assembly of insects on a tree is mostly driven by trophic interactions controlled by a mosaic of processes playing out over very different time scales. Comparisons with the literature further suggest that, for oak trees, the consequences of growing amongst distantly related tree species may depend on factors such as geographic region and tree age.
Usefulness of Longitudinal Strain Adjusted to Regional Thickness in Hypertrophic Cardiomyopathy
Authors: Urtado Sophie , Hergault Hélène , Binsse Stephen , Aidan Vincent , Ouadahi Mounir , Szymanski Catherine , Mallet Sophie , Hauguel-Moreau Marie , Carlier Robert Yves , Dubourg Olivier , Mansencal Nicolas
Published: April 2022

Background. We assessed the usefulness of a longitudinal strain adjusted to regional thickness in hypertrophic cardiomyopathy (HCM). Indeed, with conventional software, the width of the region of interest (ROI) is the same over the entire myocardial wall, wherein the software analyzes only partially the left ventricular (LV) hypertrophic segments. Methods. We included 110 patients: 55 patients with HCM (HCM group) and 55 healthy subjects (age- and sex-matched control group). The global longitudinal strain (GLS) and regional strain for each of the 17 segments was calculated with standard software (for two groups) and with software adjusted to the myocardial wall thickness (for the HCM group). Results. GLS was significantly decreased in the HCM group compared to the control group (−15.1 ± 4.8% versus −20.5 ± 4.3%, p < 0.0001). In the HCM group, GLS (standard method versus adjusted to thickness) measurements were not significantly different (p = 0.34). Interestingly, the regional strain adjusted to thickness was significantly lower than the standard strain in the hypertrophic segments, especially in the basal inferoseptal segment (p = 0.0002), median inferoseptal segment (p < 0.001) and median anteroseptal segment (p = 0.02). The strain adjusted to thickness was still significantly lower in the most hypertrophic segments (≥20 mm) (−3.7 ± 3%, versus −5.9 ± 4.4%, p = 0.049 in the basal inferoseptal segment and −5.7 ± 3.5% versus −8.3 ± 4.5%, p = 0.0007 in the median inferoseptal segment). In the segments with significant myocardial fibrosis, the longitudinal strain adjusted to thickness was significantly lower than the conventional strain (−8.3 ± 3.3% versus −11.4 ± 4.5%, p = 0.002). The analysis of the strain adjusted to thickness had a better feasibility (97.5% versus 99%, p = 0.01). Conclusions. The analysis of a longitudinal strain adjusted to regional thickness is feasible in HCM and allows a better evaluation of myocardial deformation, especially in the most LV hypertrophic segments.
A Therapeutic Pathway in Patients with Chronic Coronary Syndromes: Proposal for Optimization
Authors: De Caterina Raffaele , Calabrò Paolo , Campo Gianluca , Rossini Roberta , Giubilato Simona
Published: April 2022

There is uncertainty in cardiologists’ attitudes for prolonging dual antiplatelet therapy (DAPT) with ticagrelor 60 mg beyond 12 months in post-myocardial infarction (MI) patients. We aimed at characterizing the Italian cardiologists’ perceptions and needs in the management of such patients. Two consecutive questionnaires were proposed between June and November 2021, and compiled by 122 and 87 Cardiologists, respectively. Agreement among cardiologists was defined as either a >70% frequency of concordant responses relative to total respondents or following the Delphi method as developed by the RAND Corporation. An agreement was reached on the indication of ticagrelor as the first choice P2Y12 inhibitor in MI patients, irrespective of the presentation [ST elevation MI (STEMI), 72%, vs. non-ST elevation MI (NSTEMI), 71%] or the management [invasive vs. conservative (75%)]. A consensus was also achieved on the possibility to consider a patient suitable for long-term DAPT with ticagrelor 60 mg even in case of another P2Y12 inhibitor used in the first year after the acute event (74, 85%). To define ischemic and bleeding risks, a consensus was reached on the utilization of one or more scores (87, 71%).
Diabetes Does Not Increase the Risk of Hospitalization Due to COVID-19 in Patients Aged 50 Years or Older in Primary Care—APHOSDIAB—COVID-19 Multicenter Study
Authors: Orozco-Beltrán Domingo , Merino-Torres Juan Francisco , Pérez Antonio , Cebrián-Cuenca Ana M. , Párraga-Martínez Ignacio , Ávila-Lachica Luis , Rojo-Martínez Gemma , Pomares-Gómez Francisco J. , Álvarez-Guisasola Fernando , Sánchez-Molla Manuel , Gutiérrez Felix , Ortega Francisco J. , Mata-Cases Manel , Carretero-Anibarro Enrique , Vilaseca Josep Maria , Quesada Jose A.
Published: April 2022

The purpose of this study was to identify clinical, analytical, and sociodemographic variables associated with the need for hospital admission in people over 50 years infected with SARS-CoV-2 and to assess whether diabetes mellitus conditions the risk of hospitalization. A multicenter case-control study analyzing electronic medical records in patients with COVID-19 from 1 March 2020 to 30 April 2021 was conducted. We included 790 patients: 295 cases admitted to the hospital and 495 controls. Under half (n = 386, 48.8%) were women, and 8.5% were active smokers. The main comorbidities were hypertension (50.5%), dyslipidemia, obesity, and diabetes (37.5%). Multivariable logistic regression showed that hospital admission was associated with age above 65 years (OR from 2.45 to 3.89, ascending with age group); male sex (OR 2.15, 95% CI 1.47–3.15), fever (OR 4.31, 95% CI 2.87–6.47), cough (OR 1.89, 95% CI 1.28–2.80), asthenia/malaise (OR 2.04, 95% CI 1.38–3.03), dyspnea (4.69, 95% CI 3.00–7.33), confusion (OR 8.87, 95% CI 1.68–46.78), and a history of hypertension (OR 1.61, 95% CI 1.08–2.41) or immunosuppression (OR 4.97, 95% CI 1.45–17.09). Diabetes was not associated with increased risk of hospital admission (OR 1.18, 95% CI 0.80–1.72; p = 0.38). Diabetes did not increase the risk of hospital admission in people over 50 years old, but advanced age, male sex, fever, cough, asthenia, dyspnea/confusion, and hypertension or immunosuppression did.
Acid-Base Disorders in COVID-19 Patients with Acute Respiratory Distress Syndrome
Authors: Chiumello Davide , Pozzi Tommaso , Fratti Isabella , Modafferi Leo , Montante Marialaura , Papa Giuseppe Francesco Sferrazza , Coppola Silvia
Published: April 2022

Our aim was to investigate the distribution of acid-base disorders in patients with COVID-19 ARDS using both the Henderson–Hasselbalch and Stewart’s approach and to explore if hypoxemia can influence acid-base disorders. COVID-19 ARDS patients, within the first 48 h of the need for a non-invasive respiratory support, were retrospectively enrolled. Respiratory support was provided by helmet continuous positive airway pressure (CPAP) or by non-invasive ventilation. One hundred and four patients were enrolled, 84% treated with CPAP and 16% with non-invasive ventilation. Using the Henderson–Hasselbalch approach, 40% and 32% of patients presented respiratory and metabolic alkalosis, respectively; 13% did not present acid-base disorders. Using Stewart’s approach, 43% and 33% had a respiratory and metabolic alkalosis, respectively; 12% of patients had a mixed disorder characterized by normal pH with a lower SID. The severe hypoxemic and moderate hypoxemic group presented similar frequencies of respiratory and metabolic alkalosis. The most frequent acid-base disorders were respiratory and metabolic alkalosis using both the Henderson–Hasselbalch and Stewart’s approach. Stewart’s approach detected mixed disorders with a normal pH probably generated by the combined effect of strong ions and weak acids. The impairment of oxygenation did not affect acid-base disorders.
Genetic Insights into Primary Restrictive Cardiomyopathy
Authors: Brodehl Andreas , Gerull Brenda
Published: April 2022

Restrictive cardiomyopathy is a rare cardiac disease causing severe diastolic dysfunction, ventricular stiffness and dilated atria. In consequence, it induces heart failure often with preserved ejection fraction and is associated with a high mortality. Since it is a poor clinical prognosis, patients with restrictive cardiomyopathy frequently require heart transplantation. Genetic as well as non-genetic factors contribute to restrictive cardiomyopathy and a significant portion of cases are of unknown etiology. However, the genetic forms of restrictive cardiomyopathy and the involved molecular pathomechanisms are only partially understood. In this review, we summarize the current knowledge about primary genetic restrictive cardiomyopathy and describe its genetic landscape, which might be of interest for geneticists as well as for cardiologists.
First-Trimester Fetal Hepatic Artery Examination for Adverse Outcome Prediction
Authors: Czuba Bartosz , Tousty Piotr , Cnota Wojciech , Borowski Dariusz , Jagielska Agnieszka , Dubiel Mariusz , Fuchs Anna , Fraszczyk-Tousty Magda , Dzidek Sylwia , Kajdy Anna , Świercz Grzegorz , Kwiatkowski Sebastian
Published: April 2022

Objective: To assess whether there are differences in first-trimester fetal hepatic artery flows depending on pregnancy outcomes. Methods: The prospective study conducted in 2012–2020 included 1841 fetuses from singleton pregnancies assessed during the routine first-trimester ultrasound examination (between 11- and 14-weeks’ gestation). Also, each fetus was examined to determine their hepatic artery flows by measuring the artery’s pulsatility index (HA-PI) and peak systolic velocity (HA-PSV). Results: The fetuses that were classified as belonging to the adverse pregnancy outcome group (those with karyotype abnormalities and congenital heart defects) were characterized by a significantly lower HA-PI and higher HA-PSV compared to normal outcome fetuses. Conclusion: Hepatic artery flow assessment proved to be a very useful tool in predicting adverse pregnancy outcomes, in particular karyotype abnormalities and congenital heart defects.
Cardiac Tomography and Cardiac Magnetic Resonance to Predict the Absence of Intracardiac Thrombus in Anticoagulated Patients Undergoing Atrial Fibrillation Ablation
Authors: Zaraket Fatima , Bas Deva , Jimenez Jesus , Casteigt Benjamin , Benito Begoña , Martí-Almor Julio , Conejos Javi , Tizón-Marcos Helena , Mojón Diana , Vallès Ermengol
Published: April 2022

Background: Pulmonary veins isolation (PVI) is a standard treatment for recurrent atrial fibrillation (AF). Uninterrupted anticoagulation for a minimum of 3 weeks before ablation and exclusion of left atrial (LA) thrombus with transesophageal echography (TEE) immediately before or during the procedure minimize peri-procedural risk. We aimed to demonstrate the utility of cardiac tomography (CT) and cardiac magnetic resonance (CMR) to rule out LA thrombus prior to PVI. Methods: Patients undergoing PVI for recurrent AF were retrospectively evaluated. Only patients that started anticoagulation at least 3 weeks prior to the CT/CMR and subsequently uninterrupted until the ablation procedure were selected. An intracardiac echo (ICE) catheter was used in all patients to evaluate LA thrombus. The results of CT/CMR were compared to ICE imaging. Results: We included 272 consecutive patients averaging 54.5 years (71% male; 30% persistent AF). Average CHA2DS2VASC score was 0.9 ± 0.83 and mean LA diameter was 42 ± 5.7 mm, 111 (41%) patients were on Acenocumarol and 161 (59%) were on direct oral anticoagulants. Anticoagulation was started 227 ± 392 days before the CT/CMR, and 291 ± 416 days before the ablation procedure. CT/CMR diagnosed intracardiac thrombus in two cases, both in the LA appendage. A new CT/CMR revealed resolution of thrombus after six additional months of uninterrupted anticoagulation. No macroscopic thrombus was observed in any patients with ICE (negative predictive value of 100%; p < 0.01). Conclusions: CT and MRI are excellent surrogates to TEE and ICE to rule out intracardiac thrombus in patients adequately anticoagulated prior AF ablation. This is true even for delayed procedures as long as anticoagulation is uninterrupted.
Effects of Magnetic Abrasive Finishing on Microstructure and Mechanical Properties of Inconel 718 Processed by Laser Powder Bed Fusion
Authors: Zhao Yunhao , Ratay Jason , Li Kun , Yamaguchi Hitomi , Xiong Wei
Published: April 2022

Surface finishing is challenging in the context of additively manufactured components with complex geometries. Magnetic abrasive finishing (MAF) is a promising surface finishing technology that can refine the surface quality of components with complex shapes produced by additive manufacturing. However, there is insufficient study regarding the impact of MAF on microstructure–property relationships for additively manufactured builds, which is critical for evaluating mechanical performance. In this work, we studied the effects of different combinations of MAF and heat treatment steps on the microstructure–property relationships of Inconel 718 superalloys made by laser powder bed fusion (LPBF). The application of MAF was found to significantly reduce the surface roughness and refine the grain size of aged alloys. Moreover, MAF was able to increase the alloy elongation, which could be further influenced by the sequence of MAF and different heat treatment steps. The highest elongation could be achieved when MAF was performed between homogenization and aging processes. This work indicates that an effective combination of surface finishing and heat treatment is critical for the improvement of alloy performance. Furthermore, it demonstrates a promising solution for improving the performance of LPBF Inconel 718 by integrating MAF and heat treatment, which provides new perspectives on the post-processing optimization of additively manufactured alloys.
Adaptive Formation Control of Unmanned Underwater Vehicles with Collision Avoidance under Unknown Disturbances
Authors: Yan Zheping , Jiang Anzuo , Lai Chonglang
Published: April 2022

In this paper, the formation control problem for a group of unmanned underwater vehicles (UUVs) is investigated considering collision avoidance and environment disturbances. To address the external force effect of the environment, such as waves and currents, a sliding mode disturbance observer is designed to compensate for the unknown dynamic disturbances in finite time. A bounded artificial potential field is incorporated into the control law to ensure collision avoidance among UUVs. The form of an artificial potential function is much simpler and convenient for engineering applications. A controller is devised to guarantee all the error signals are bounded, and the formation pattern can be achieved in finite time after collision avoidance. The stability of UUV formation with collision avoidance is proven by using the Lyapunov theorem, and the scheme has been shown to be convergent using Barbalat’s lemma. Comparative simulations are presented to demonstrate the effectiveness of the proposed method in 2-D and 3-D environments.
Taking a Closer Look: Clinical and Histopathological Characteristics of Culture-Positive versus Culture-Negative Pulmonary Mucormycosis
Authors: Spallone Amy , Moran Cesar A. , Wurster Sebastian , Axell-House Dierdre B. , Kontoyiannis Dimitrios P.
Published: April 2022

The cultural recovery of Mucorales from hyphae-laden tissue is poor, and the clinical implications of culture positivity are scarcely studied. Therefore, we compared clinical and histopathological characteristics of culture-positive and culture-negative histology-proven pulmonary mucormycosis cases among cancer patients. Histology specimens were blindly reviewed by a thoracic pathologist and graded on four histopathologic features: hyphal quantity, tissue necrosis, tissue invasion, and vascular invasion. Twenty cases with a corresponding fungal culture were identified; five were culture-positive, and fifteen were culture-negative. Although no statistically significant differences were found, culture-positive patients were more likely to exhibit a high burden of necrosis and have a high burden of hyphae but tended to have less vascular invasion than culture-negative patients. In terms of clinical characteristics, culture-positive patients were more likely to have acute myeloid leukemia (60% vs. 27%, p = 0.19), a history of hematopoietic cell transplant (80% vs. 53%, p = 0.31), severe lymphopenia (absolute lymphocyte count ≤ 500/µL, 100% vs. 73%, p = 0.36), and monocytopenia (absolute monocyte count ≤100/µL, 60% vs. 20%, p = 0.11). Forty-two-day all-cause mortality was comparable between culture-positive and culture-negative patients (60% and 53%, p = 0.80). This pilot study represents the first comprehensive histopathological scoring method to examine the relationship between histopathologic features, culture positivity, and clinical features of pulmonary mucormycosis.
Multidisciplinary Consulting Team for Complicated Cases of Neurodevelopmental and Neurobehavioral Disorders: Assessing the Opportunities and Challenges of Integrating Pharmacogenomics into a Team Setting
Authors: Gill Pritmohinder S. , Elchynski Amanda L. , Porter-Gill Patricia A. , Goodson Bradley G. , Scott Mary Ann , Lipinski Damon , Seay Amy , Kehn Christina , Balmakund Tonya , Schaefer G. Bradley
Published: April 2022

Neurodevelopmental disorders have steadily increased in incidence in the United States. Over the past decade, there have been significant changes in clinical diagnoses and treatments some of which are due to the increasing adoption of pharmacogenomics (PGx) by clinicians. In this pilot study, a multidisciplinary team at the Arkansas Children’s Hospital North West consulted on 27 patients referred for difficult-to-manage neurodevelopmental and/or neurobehavioral disorders. The 27 patients were evaluated by the team using records review, team discussion, and pharmacogenetic testing. OneOme RightMed® (Minneapolis, MN, USA) and the Arkansas Children’s Hospital comprehensive PGx test were used for drug prescribing guidance. Of the 27 patients’ predicted phenotypes, the normal metabolizer was 11 (40.8%) for CYP2C19 and 16 (59.3%) for CYP2D6. For the neurodevelopmental disorders, the most common comorbid conditions included attention-deficit hyperactivity disorder (66.7%), anxiety disorder (59.3%), and autism (40.7%). Following the team assessment and PGx testing, 66.7% of the patients had actionable medication recommendations. This included continuing current therapy, suggesting an appropriate alternative medication, starting a new therapy, or adding adjunct therapy (based on their current medication use). Moreover, 25.9% of patients phenoconverted to a CYP2D6 poor metabolizer. This retrospective chart review pilot study highlights the value of a multidisciplinary treatment approach to deliver precision healthcare by improving physician clinical decisions and potentially impacting patient outcomes. It also shows the feasibility to implement PGx testing in neurodevelopmental/neurobehavioral disorders.
Clinical Outcomes of Cardiac Rehabilitation in Women with Coronary Artery Disease—Differences in Comparison with Men
Authors: Szmigielska Katarzyna , Jegier Anna
Published: April 2022

This study evaluated the clinical outcomes of cardiac rehabilitation (CR) in women with coronary artery disease (CAD) in comparison to men. Methods: Patients after acute coronary syndrome or after revascularization procedures (106 women, 180 men) were consecutively admitted to a comprehensive outpatient CR program, comprising of 45-min ergometer interval training three times a week for eight weeks. The training intensity was determined on the basis of training heart rate, calculated following an exercise test. Patients were divided into subgroups according to age (≤55, >55 years), BMI (<25, ≥25 kg/m2), left ventricular ejection fraction (LVEF; ≤40%, 41–49%, ≥50%), and number of affected coronary vessels. Results: After eight weeks, exercise capacity increased significantly by 0.6 ± 0.77 MET (women) and by 1.0 ± 0.74 MET (men). The greatest benefit was observed in men, women under 55 years, women with LVEF 41–49%, and women with single-vessel CAD. An outpatient CR program appears less beneficial for women, especially those over 55 years, with two or three coronary vessels affected with atherosclerosis or with LVEF > 50%. In women with CAD, eight weeks of 45-min interval training, with sessions three times a week, is insufficient to improve exercise capacity to an extent that is considered a predictor of mortality risk reduction.
Machine-Learning-Based Late Fusion on Multi-Omics and Multi-Scale Data for Non-Small-Cell Lung Cancer Diagnosis
Authors: Carrillo-Perez Francisco , Morales Juan Carlos , Castillo-Secilla Daniel , Gevaert Olivier , Rojas Ignacio , Herrera Luis Javier
Published: April 2022

Differentiation between the various non-small-cell lung cancer subtypes is crucial for providing an effective treatment to the patient. For this purpose, machine learning techniques have been used in recent years over the available biological data from patients. However, in most cases this problem has been treated using a single-modality approach, not exploring the potential of the multi-scale and multi-omic nature of cancer data for the classification. In this work, we study the fusion of five multi-scale and multi-omic modalities (RNA-Seq, miRNA-Seq, whole-slide imaging, copy number variation, and DNA methylation) by using a late fusion strategy and machine learning techniques. We train an independent machine learning model for each modality and we explore the interactions and gains that can be obtained by fusing their outputs in an increasing manner, by using a novel optimization approach to compute the parameters of the late fusion. The final classification model, using all modalities, obtains an F1 score of 96.81±1.07, an AUC of 0.993±0.004, and an AUPRC of 0.980±0.016, improving those results that each independent model obtains and those presented in the literature for this problem. These obtained results show that leveraging the multi-scale and multi-omic nature of cancer data can enhance the performance of single-modality clinical decision support systems in personalized medicine, consequently improving the diagnosis of the patient.
Severe Aortic Valve Stenosis and Pulmonary Hypertension: A Systematic Review of Non-Invasive Ways of Risk Stratification, Especially in Patients Undergoing Transcatheter Aortic Valve Replacement
Authors: Boxhammer Elke , Berezin Alexander E. , Paar Vera , Bacher Nina , Topf Albert , Pavlov Sergii , Hoppe Uta C. , Lichtenauer Michael
Published: April 2022

Patients with severe aortic valve stenosis and concomitant pulmonary hypertension show a significantly reduced survival prognosis. Right heart catheterization as a preoperative diagnostic tool to determine pulmonary hypertension has been largely abandoned in recent years in favor of echocardiographic criteria. Clinically, determination of echocardiographically estimated systolic pulmonary artery pressure falls far short of invasive right heart catheterization data in terms of accuracy. The aim of the present systematic review was to highlight noninvasive possibilities for the detection of pulmonary hypertension in patients with severe aortic valve stenosis, with a special focus on cardiovascular biomarkers. A total of 525 publications regarding echocardiography, cardiovascular imaging and biomarkers related to severe aortic valve stenosis and pulmonary hypertension were analyzed in a systematic database analysis using PubMed Central®. Finally, 39 publications were included in the following review. It was shown that the current scientific data situation, especially regarding cardiovascular biomarkers as non-invasive diagnostic tools for the determination of pulmonary hypertension in severe aortic valve stenosis patients, is poor. Thus, there is a great scientific potential to combine different biomarkers (biomarker scores) in a non-invasive way to determine the presence or absence of PH.
Development of Personalized Non-Invasive Ventilation Interfaces for Neonatal and Pediatric Application Using Additive Manufacturing
Authors: Bockstedte Marit , Xepapadeas Alexander B. , Spintzyk Sebastian , Poets Christian F. , Koos Bernd , Aretxabaleta Maite
Published: April 2022

The objective of this study was to present a methodology and manufacturing workflow for non-invasive ventilation interfaces (NIV) for neonates and small infants. It aimed to procure a fast and feasible solution for personalized NIV produced in-house with the aim of improving fit and comfort for the patient. Three-dimensional scans were obtained by means of an intraoral (Trios 3) and a facial scanner (3dMd Flex System). Fusion 360 3D-modelling software was employed to automatize the design of the masks and their respective casting molds. These molds were additively manufactured by stereolithography (SLA) and fused filament fabrication (FFF) technologies. Silicone was poured into the molds to produce the medical device. In this way, patient individualized oronasal and nasal masks were produced. An automated design workflow and use of additive manufacturing enabled a fast and feasible procedure. Despite the cost for individualization likely being higher than for standard masks, a user-friendly workflow for in-house manufacturing of these medical appliances proved to have potential for improving NIV in neonates and infants, as well as increasing comfort.
Association between Gout and Dyslipidemia: A Nested Case–Control Study Using a National Health Screening Cohort
Authors: Choi Hyo Geun , Kwon Bong-Cheol , Kwon Mi Jung , Kim Ji Hee , Kim Joo-Hee , Park Bumjung , Lee Jung Woo
Published: April 2022

The association between lipid levels and uric acid disorders remains controversial. We evaluated the association between dyslipidemia and gout in a large cohort from the Korean National Health Insurance Service-Health Screening Cohort. Among the 514,866 participants aged ≥40 years, 16,679 gout participants were selected and matched with 66,716 control participants for income, region of residence, sex, and age. We used the ICD-10 codes to define dyslipidemia (E78) and gout (M10) and diagnosis was confirmed when each was reported ≥2 times. The odds ratios (ORs) of dyslipidemia history were calculated using conditional logistic regression in crude, partial, and fully adjusted models. The days of statin use, systolic and diastolic blood pressure, fasting glucose level, total cholesterol, obesity, Charlson comorbidity index, alcohol consumption, and smoking were used as covariates. Patients with gout had a significantly higher dyslipidemia history than those without gout (33.1% vs. 24.0%, p < 0.001). The association was significant after adjustment (OR in partial adjusted model = 1.50, 95% confidence interval (CI) = 1.44–1.57; OR in fully adjusted model = 1.43, 95% CI = 1.37–1.49). These findings were consistent with the subgroup analysis. Our findings suggest that dyslipidemia history is more likely in patients with gout aged ≥40 years than in healthy controls among Korean population.
Does Observer Presence Modify the Behavior and Enclosure Use of Captive Edwards’ Pheasants?
Authors: Hoy Rhiannon Amy , Brereton James Edward
Published: April 2022

It is well known that captive animals alter their behavior and space use when observed by visitors, with the concept coined the ‘visitor effect’. The ‘observer effect’, described as any alteration in behavior and enclosure use as a result of a quiet, stationary observer, has been less studied. This study investigates the observer effect in two pairs of Edwards’ pheasants (Lophura edwardsi) and their offspring at Sparsholt College, United Kingdom. The impact of an observer (as opposed to camera) on behavior and enclosure use of pheasants was observed, using instantaneous focal sampling. Enclosure use was measured by converting both enclosures into unequal zones and then assessing the evenness of enclosure use through modified Spread of Participation Index. Poisson regression analysis was used to investigate observer impact, alongside the additional variables of keeper and visitor presence, temperature, and individual bird differences. Overall, the behaviors of resting and clustering were significantly increased during observer presence, whereas feeding and locomotion were significantly decreased. The behaviors of preening and standing were not affected by observer presence, though they were influenced by keeper and visitor presence. Enclosure use was also affected by observer presence, though the effect size was small. This suggests that pheasants may perceive the presence of humans near their enclosures as a potential threat, and may alter their behavior to reduce detection, similar to their wild counterparts. Animal researchers should consider the potential impact of observer presence on their subjects, particularly when observing species such as pheasants.
Quantitatively Calculating the Contribution of Vegetation Variation to Runoff in the Middle Reaches of Yellow River Using an Adjusted Budyko Formula
Authors: Ji Guangxing , Huang Junchang , Guo Yulong , Yan Dan
Published: April 2022

The middle reaches of the Yellow River (MRYR) are a key area for carrying out China’s vegetation restoration project. However, the impact of vegetation variation on runoff in the MRYR is still unclear. For quantitatively evaluating the contribution rate of vegetation variation to runoff in the MRYR, this paper quantified the relationship between Normalized Difference Vegetation Index (NDVI) and Budyko parameters (w). Then, we used multiple linear regression to quantitatively calculate the contribution rate of different factors on vegetation variation. Finally, an adjusted Budyko formula was constructed to quantitatively calculate the influence of vegetation variation on runoff. The results showed that there is a linear relationship between NDVI and Budyko parameters (w) (p < 0.05); the fitting parameter and constant term were 12.327 and −0.992, respectively. Vegetation change accounted for 33.37% in the MRYR. The contribution of climatic and non-climatic factors on vegetation change is about 1:99. The contribution of precipitation, potential evaporation, anthropogenic activities on the runoff variation in the MRYR are 23.07%, 13.85% and 29.71%, respectively.
Response of Surface Runoff and Sediment to the Conversion of a Marginal Grassland to a Switchgrass (Panicum virgatum) Bioenergy Feedstock System
Authors: Zou Chris B. , Lambert Lixia H. , Everett Josh , Will Rodney E.
Published: April 2022

The land systems between the humid and arid zones around the globe are critical to agricultural production and are characterized by a strong integration of the land use and water dynamics. In the southern Great Plains (SGP) of the United States, lakes and farm ponds are essential components in the land systems, and they provide unique habitats for wildlife, and critical water resources for irrigation and municipal water supplies. The conversion of the marginal grasslands to switchgrass (Panicum virgatum) biofuel feedstock for energy production has been proposed in the region. However, we have limited experimental data to assess the impact of this potential land-use change on the surface runoff, which is the primary water source for surface impoundments. Here, we report the results from a paired experimental watershed study that compared the runoff and sediment responses that were related to the conversion of prairie to a low-input biomass production system. The results show no significant change in the relationship between the event-based runoff and the precipitation. There was a substantial increase in the sediment yield (328%) during the conversion phase that was associated with the switchgrass establishment (i.e., the site preparation, herbicide application, and switchgrass planting). Once the switchgrass was established, the sediment yield was 21% lower than the nonconverted watershed. Our site-specific observations suggest that switchgrass biofuel production systems will have a minimum impact on the existing land and water systems. It may potentially serve as an environmentally friendly and economically viable alternative land use for slowing woody encroachment on marginal lands in the SGP.
Water Diversion in the Valley of Mexico Basin: An Environmental Transformation That Caused the Desiccation of Lake Texcoco
Authors: Montero-Rosado Carolina , Ojeda-Trejo Enrique , Espinosa-Hernández Vicente , Fernández-Reynoso Demetrio , Caballero Deloya Miguel , Benedicto Valdés Gerardo Sergio
Published: April 2022

Mexico’s basin is one of the most altered in the country, owing to the presence of the megalopolis of Mexico City. Lake Texcoco, which had the basin’s biggest extension, dried up almost completely. The basin’s evolution over time led in the formation of a megabasin in which water is transported from one source to another to serve the urban region and subsequently drained to prevent flooding. The major hydrotechnical works in Mexico Basin have been interpreted as a solution to the problem of flooding in Mexico City, but they were actually part of a much larger strategy of territorial appropriation by the Spanish colonists. The ecological imbalance that has resulted has sparked a variety of social issues. For the purpose of analyzing the environmental transformation of Lake Texcoco over the last 500 years, actors and processes that influenced specific moments in the country’s history were identified; these elements showed the inexorable relationship between the lake and Mexico City. Subsequently, they were grouped by periods with similar trends in terms of the way in which society relates to and appropriates the natural environment of the lake. It was found that the critical moment for the desiccation of Lake Texcoco occurred during the Spanish colonial historical period as part of the redesign of the city; from then on, the same environmental imaginary prevailed century after century, shaped by social and economic factors. This study contributes to the literature on how urbanization affects natural resources by making an original theoretical contribution through an analysis based on political ecology, and it adds to the literature on how people use the prevailing federal area of the lake.
Quantifying the Aboveground Biomass (AGB) of Gobi Desert Shrub Communities in Northwestern China Based on Unmanned Aerial Vehicle (UAV) RGB Images
Authors: Ding Jie , Li Zhipeng , Zhang Heyu , Zhang Pu , Cao Xiaoming , Feng Yiming
Published: April 2022

Shrubs are an important part of the Gobi Desert ecosystem, and their aboveground biomass (AGB) is an important manifestation of the productivity of the Gobi Desert ecosystem. Characterizing the biophysical properties of low-stature vegetation such as shrubs in the Gobi Desert via conventional field surveys and satellite remote sensing images is challenging. The AGB of shrubs had been estimated from spectral variables taken from high-resolution images obtained by unmanned aerial vehicle (UAV) in the Gobi Desert, Xinjiang, China, using vegetation feature metrics. The main results were as follows: (1) Based on the UAV images, several RGB vegetation indices (RGB VIs) were selected to extract the vegetation coverage, and it was found that the excess green index (EXG) had the highest accuracy and the overall extraction accuracy of vegetation coverage reached 97.00%. (2) According to field sample plot surveys, the AGB and shrub crown area of single shrubs in the Gobi Desert were in line with a power model. From the bottom of the alluvial fan to the top of the alluvial fan, as the altitude increased, the AGB of the vegetation communities showed an increasing trend: the AGB of the vegetation communities at the bottom of the alluvial fan was 2–90 g/m2, while that at the top of the alluvial fan was 60–201 g/m2. (3) Vegetation coverage (based on the UAV image EXG index) and AGB showed a good correlation. The two conform to the relationship model (R2 = 0.897) and the expression is Y = 1167.341 x0.946, where Y is the AGB of the sample plots in units g/m2 and x is the vegetation coverage extracted by the VI. (4) The predicted AGB values of Gobi Desert shrubs using UAV RGB images based on a power model were closer to the actual observed AGB values. The study findings provide a more efficient, accurate, and low-cost method for estimating vegetation coverage and AGB of Gobi Desert shrubs.
Exploring Adaptive UHI Mitigation Solutions by Spatial Heterogeneity of Land Surface Temperature and Its Relationship to Urban Morphology in Historical Downtown Blocks, Beijing
Authors: Zhang Liukuan , Shi Xiaoxiao , Chang Qing
Published: April 2022

Heat stress brought on by the intensification of urban heat island (UHI) has caused many negative effects on human beings, which were found to be more severe in highly urbanized old towns. With the inconsistent findings on how urban spatial morphological characteristics influence land surface temperature (LST) and gaps between design practices being found, we chose Beijing Old Town (BOT) as the study area and took the basic planning implementation module “block” as a study to reveal the spatial heterogeneity of LST and its relationship to multiple urban morphological characteristics with higher spatial resolution calculated via WorldView3. Our results have shown that (1) UHI effect was significant and spatially heterogeneous in BOT, and significant hot areas with high LST value and small LST differences were found, as cold areas were the exact opposite. (2) The proportion of vegetated area, water, impervious surface, and urban spatial structure indicators i.e., building coverage ratio, mean height, highest building index, height fluctuation degree, space crowd degree and sky view factor were identified as significantly affecting the LST of blocks in BOT. (3) The effects of GBI components and configuration on LST varied within different block types; generally, blocks with GBI with larger patches that were more complex in shape, more aggregated, and less fragmented were associated with lower LST. Finally, in the context of integrating our study results with relevant planning and design guidelines, a strategy sample of adaptive GBI planning and vegetation design for blocks with different morphological features was provided for urban planners and managers to make a decision on UHI mitigation in the renewal process of BOT.
Evaluation of the Health Promotion Capabilities of Greenway Trails: A Case Study in Hangzhou, China
Authors: Xu Bin , Shi Qingxia , Zhang Yaping
Published: April 2022

As a type of green infrastructure, greenways are beneficial for walking and cycling and promote urban health and well-being. Taking the Qingshan Lake Greenway Phase One (QLG-I) Trail in the Lin’an District of Hangzhou city as an example and based on the accessibility of points of interest (POI) near the QLG-I Trail, a questionnaire investigation, and an importance performance analysis (IPA), in this paper, we construct a methodological framework to evaluate the health-promotion capabilities of the QLG-I Trail, including three aspects: promoting the coverage of healthy travel, user attribute analysis, and user perceptions of the greenway for health promotion. The results show that the healthy travel range of the QLG-I Trail is small and that the users are mainly residents of nearby communities. Additionally, the main factors affecting users’ health-promoting behaviour are safety, cleanliness, and infrastructure services. Although the overall satisfaction with service quality was good (3.93), we found that the trail facilities did not meet the needs of the users. This study confirms that the QLG-I Trail provides community residents with a place for sports activities and supports health-promoting behaviour. Greenway facilities and the natural environment enhance this utility; however, promoting the coverage of healthy travel is limited by accessibility. Finally, we propose a traffic-organization optimization and improvement plan for the QLG-I Trail. The research results may help promote healthy activities on this type of greenway.
Two Views of Speech Acts: Analysis and Implications for Argumentation Theory
Authors: Kauffeld Fred , Goodwin Jean
Published: April 2022

Argumentation theorists need to command a clear view of the sources of the obligations that arguers incur, e.g., their burdens of proof. Theories of illocutionary speech acts promise to fill this need. This essay contrasts two views of illocutionary acts: one, that they are constituted by rules, the other, that they are constituted by paradigmatic practical calculations. After a general comparison of the two views, the strength of the pragmatic view is demonstrated through an account of the illocutionary act of making an accusation. It is shown that the essential conditions of ACCUSING revealed by conceptual analysis are just what is practically necessary to manage a routine, but complex, communicative problem. The essay closes with remarks on the implications of the pragmatic view of speech acts for argumentation theory generally.
Bladder Cancer Cells Exert Pleiotropic Effects on Human Adipose-Derived Stem Cells
Authors: Maj Małgorzata , Kaźmierski Łukasz , Balik Karolina , Kowalska Karolina , Gackowska Lidia , Bajek Anna , Drewa Tomasz
Published: April 2022

Stem cell-based therapies are considered one of the most promising disciplines in biomedicine. Bladder cancer patients could benefit from therapies directed to promote healing after invasive surgeries or to lessen urinary incontinence, a common side effect of both cancer itself and the treatment. However, the local delivery of cells producing large amounts of paracrine factors may alter interactions within the microenvironment. For this reason, reconstructive cellular therapies for patients with a history of cancer carry a potential risk of tumor reactivation. We used an indirect co-culture model to characterize the interplay between adipose-derived stem cells and bladder cancer cells. Incubation with ASCs increased MCP-1 secretion by bladder cancer cells (from 2.1-fold to 8.1-fold, depending on the cell line). Cancer cell-derived factors altered ASC morphology. Cells with atypical shapes and significantly enlarged volumes appeared within the monolayer. Incubation in a conditioned medium (CM) containing soluble mediators secreted by 5637 and HB-CLS-1 bladder cancer cell lines decreased ASC numbers by 47.5% and 45.7%. A significant increase in adhesion to ECM components, accompanied by reduced motility and sheet migration, was also observed after incubation in CM from 5637 and HB-CLS-1 cells. No differences were observed when ASCs were co-cultured with HT-1376 cells. Our previous and present results indicate that soluble mediators secreted by ASCs and bladder cancer cells induce opposite effects influencing cells that represent the non-muscle-invasive urinary bladder.
Endothelial-Derived APT1-Mediated Macrophage-Endothelial Cell Interactions Participate in the Development of Atherosclerosis by Regulating the Ras/MAPK Signaling Pathway
Authors: Wang Xinghua , Cheng Lijun , Fu Huaying , Chan Calista Zhuo Yi , Tse Gary , Liu Tong , Li Guangping
Published: April 2022

Acyl-protein thioesterase 1 (APT1) can affect H-Ras localization and function by promoting its depalmitoylation. However, relatively little attention has been paid to the effects of APT1 on H-Ras in the cardiovascular system. In this study, we revealed its roles in atherosclerosis development using oxidative low-density lipoprotein (ox-LDL)-induced endothelial dysfunction models and a Western diet-induced ApoE−/− mouse model. The results showed that APT1 expression was up-regulated, while that of miR-138-5p (miR-138) was down-regulated (p < 0.05) in this model. In the meantime, APT1 and H-Ras were translocated from the cytoplasm to the plasma membrane. Bioinformatic analysis and double fluorescence identified miR-138 as the upstream regulator of APT1. APT1 knockdown regulated H-Ras localization and expression, which subsequently affected the MAPK signaling pathway and the expression of its downstream factors. Further research indicated that human umbilical vein endothelial cells (HUVECs)-derived biogenic nanoparticles (BiNPs), hBPs secretion, and RNA expression of hBP-loaded APT1 were increased (p < 0.05) in the ox-LDL induced endothelial dysfunction model. Meanwhile, the HUVECs-derived APT1 could further affect macrophage function through hBP transportation. Altogether, this study demonstrated that the miR-138-APT1 axis may be partially responsible for atherosclerosis development by regulating the H-Ras-MAPK signaling pathway and hBP transportation. The results also shed novel insight on the underlying mechanisms of, and identify potential diagnostic and therapeutic targets for, atherosclerotic cardiovascular diseases in the future.
Abbreviated MR Protocols in Prostate MRI
Authors: Hötker Andreas M. , Vargas Hebert Alberto , Donati Olivio F.
Published: April 2022

Prostate MRI is an integral part of the clinical work-up in biopsy-naïve patients with suspected prostate cancer, and its use has been increasing steadily over the last years. To further its general availability and the number of men benefitting from it and to reduce the costs associated with MR, several approaches have been developed to shorten examination times, e.g., by focusing on sequences that provide the most useful information, employing new technological achievements, or improving the workflow in the MR suite. This review highlights these approaches; discusses their implications, advantages, and disadvantages; and serves as a starting point whenever an abbreviated prostate MRI protocol is being considered for implementation in clinical routine.