bluuebunny/crossref_metadata_2025 huggingface dataset:
Characterization of continuum robot arms under reinforcement learning and derived improvements
Authors: Morimoto Ryota , Ikeda Masahiro , Niiyama Ryuma , Kuniyoshi Yasuo
Published: September 2022

In robotics, soft continuum robot arms are a promising prospect owing to their redundancy and passivity; however, no comprehensive study exists that examines their characteristics compared to rigid manipulators. In this study, we examined the advantages of a continuum robot arm as compared to a typical and rigid seven-degree-of-freedom (7-DoF) robot manipulator in terms of performing various tasks through reinforcement learning. We conducted simulations for tasks with different characteristics that require control over position and force. Common tasks in robot manipulators, such as reaching, crank rotation, object throwing, and peg-in-hole were considered. The initial conditions of the robot and environment were randomized, aiming for evaluations including robustness. The results indicate that the continuum robot arm excels in the crank-rotation task, which is characterized by uncertainty in environmental conditions and cumulative rewards. However, the rigid robot arm learned better motions for the peg-in-hole task than the other tasks, which requires fine motion control of the end-effector. In the throwing task, the continuum robot arm scored well owing to its good handling of anisotropy. Moreover, we developed a reinforcement-learning method based on the comprehensive experimental results. The proposed method successfully improved the motion learning of a continuum robot arm by adding a technique to regulate the initial state of the robot. To the best of our knowledge, ours is the first reinforcement-learning experiment with multiple tasks on a single continuum robot arm and is the first report of a comparison between a single continuum robot arm and rigid manipulator on a wide range of tasks. This simulation study can make a significant contribution to the design of continuum arms and specification of their applications, and development of control and reinforcement learning methods.
How social norms contribute to physical violence among ever-partnered women in Uganda: A qualitative study
Authors: Nnyombi Aloysious , Bukuluki Paul , Besigwa Samuel , Ocaya-Irama Jane , Namara Charity , Cislaghi Beniamino
Published: September 2022

This paper contributes to the literature that studies how social norms sustain undesirable behavior. It establishes how norms contribute to intimate partner physical violence against women. First, norms organize physical violence as a domestic and private matter. Second, they organize physical violence as a constituent part of women's lives, thereby normalizing women's experience of abuse. Third, norms define appropriate boundaries within which male partners perpetrate violence. The findings draw essential information for social change interventions that target improvement in women's and girls' wellbeing. For social and behavioral programmes to change harmful norms, they have to deconstruct physical violence as a private matter, advance the de-normalization of physical violence, and dismantle acceptable boundaries within which violence happens.
A multi-modal volumetric microscope with automated sample handling for surveying microbial life in liquid samples
Authors: Kim Taewoo , Oborny Nathan , Serabyn Eugene , Wallace J. Kent , Liewer Kurt , Bedrossian Manuel , Rider Stephanie , Noell Aaron , Willis Peter , Lindensmith Chris , Nadeau Jay
Published: September 2022

In the study of microbial life, microscopy plays a unique role due to its ability to detect ordered structure, motility, and fluorescence signals. As such it has also recently gained attention in the context of searching for extant life on distant Solar System bodies bearing liquid water. In this paper we introduce a multi-modal volumetric microscopy system for potential future spaceflight missions that combines digital holographic microscopy (DHM) and volume fluorescence imager (VFI), which are volumetric imaging methods that provide high-resolution, high-throughput examination of liquid samples. DHM provides information on the absorption, morphology, and motility of imaged objects without requiring the use of contrast agents. On the other hand, VFI based on light field microscopy focuses on the fluorescence signals from the sample to observe specific structures dyed with targeted contrast agents or providing unique autofluorescence signals. We also present an autonomous sample handling and data acquisition system to allow for an autonomous mission to distant planets or moons, or for autonomous use in bodies of water on Earth. The full system, named ELVIS, or Extant Life Volumetric Imaging System, is capable of autonomously surveying a liquid sample to extract morphology, motility, and fluorescence signals of extant microbial life.
Impact of early Kasai portoenterostomy on short-term outcomes of biliary atresia: A systematic review and meta-analysis
Authors: Yang Changzhen , Ke Meng , Zhou Yan , Xu Hang , Diao Mei , Li Long
Published: September 2022

Background

Good outcomes of biliary atresia (BA) are conventionally achieved after early Kasai portoenterostomy (KP). However, in some recent pieces of literature, there are discrepancies in the influence of age in Kasai procedure on postoperative short-term prognosis. This meta-analysis aims to evaluate the effects of earlier KP on short-term surgical prognosis of BA and clarify these discrepancies in recent studies.

Methods

To identify related studies, PubMed, Embase, Web of Science, Cochrane, and the Chinese National Knowledge Infrastructure database were searched up to March 2022. Data for the impact of age at KP on clinical prognosis were extracted, including jaundice clearance rate (JCR) and native liver survival rate (NLSR).

Results

A total of 14 articles were included in the present study, which involve a total of 3,276 patients with BA who underwent Kasai procedure. Compared with patients older than 91 days of age, patients 90 days of age or younger exhibited significantly better JCR [odds ratio (OR), 3.05; 95% confidence interval (CI), 2.23–4.17; P < .001] and a more favorable NLSR (OR, 1.72; 95% CI, 1.37–2.15; P < .001). The NLSR of patients younger than 60 days of age was significantly higher than those of patients from 61 to 90 days of age (OR, 1.41; 95% CI, 1.18–1.68; P < .001). There was no significant difference in JCRs between patients aged 60 days of age or younger and those aged 61–90 days of age (OR, 1.31; 95% CI, 0.95–1.81; P = 0.10). Among patients 30 days of age or younger, 31–45 days of age, and 46–60 days of age, there were also no significant differences in JCR.

Conclusion

A significantly better short-term JCR and NLSRs were achieved among patients with BA treated using a KP procedure at ≤90 days of age compared with those treated at >90 days of age. There was no further improvement in the short-term JCR when the procedure was performed at ≤60 days compared with those treated at 61–90 days of age. However, treatment at ≤60 days of age was associated with a significant improvement in NLSR. Therefore, the timing of KP does exert an important effect on short-term clinical outcomes of patients with BA.
Consequences of inequity in the neurosurgical workforce: Lessons from traumatic brain injury
Authors: Venkatesh Shivani , Bravo Marcela , Schaaf Tory , Koller Michael , Sundeen Kiera , Samadani Uzma
Published: September 2022

Women and minorities leave or fail to advance in the neurosurgical workforce more frequently than white men at all levels from residency to academia. The consequences of this inequity are most profound in fields such as traumatic brain injury (TBI), which lacks objective measures. We evaluated published articles on TBI clinical research and found that TBI primary investigators or corresponding authors were 86·5% White and 59·5% male. First authors from the resulting publications were 92.6% white. Most study participants were male (68%). 64·4% of NIH-funded TBI clinical trials did not report or recruit any black subjects and this number was even higher for other races and the Hispanic ethnicity. We propose several measures for mitigation of the consequences of the inequitable workforce in traumatic brain injury that could potentially contribute to more equitable outcomes. The most immediately feasible of these is validation and establishment of objective measures for triage and prognostication that are less susceptible to bias than current protocols. We call for incorporation of gender and race neutral metrics for TBI evaluation to standardize classification of injury. We offer insights into how socioeconomic factors contribute to increased death rates from women and minority groups. We propose the need to study how these disparities are caused by unfair health insurance reimbursement practices. Surgical and clinical research inequities have dire consequences, and until those inequities can be corrected, mitigation of those consequences requires system wide change.
Intrahepatic sarcomatoid cholangiocarcinoma: A case report of the youngest patient on record and a review of the condition's characteristics
Authors: Xi Long-Fu , Jin Yun , Li Jiang-Tao
Published: September 2022

As a rare disease, intrahepatic sarcomatoid cholangiocarcinoma (s-CCC) represents less than 1% of malignancies of the hepatobiliary system and its main clinical symptoms include abdominal pain and fever. Results of pathological examinations, despite being the “gold standard”, can easily be confused with hepatocellular carcinoma (HCC). This report is about a 32-year-old male patient who was hospitalized due to occupancy of segment V of the liver for three days and had a history of chronic hepatitis B (CHB) over a 20-year span. Magnetic resonance imaging (MRI) showed a 43 mm × 52 mm-sized liver mass in the V segment, with patchy peripheral enhancement during the arterial phase and rapid wash-out during the portal and late phases. A laparoscopic hepatectomy of segment V, along with cholecystectomy, was performed. Histopathological and immunohistochemical examinations indicated a malignant neoplasm that was positive for vimentin and cytokeratin, with these features providing a positive diagnosis for intrahepatic sarcomatoid cholangiocarcinoma. After surgery, an adjuvant therapy of albumin-paclitaxel combined with gemcitabine regimen was given. No recurrence was found six months after the surgery, with follow-up still ongoing. This report aims to improve the awareness, diagnosis, and treatment of s-CCC.
Therapeutic effect of Internal iliac artery ligation and uterine artery ligation techniques for bleeding control in placenta accreta spectrum patients: A meta-analysis of 795 patients
Authors: Nabhan Ayman Essa , AbdelQadir Yossef Hassan , Abdelghafar Yomna Ali , Kashbour Muataz Omar , Salem Nour , Abdelkhalek Abdelrahman Naeim , Nourelden Anas Zakarya , Eshag Mona Muhe Eldeen , Shah Jaffer
Published: September 2022

Placenta accreta spectrum (PAS) can cause complications like hysterectomy or death due to massive pelvic bleeding. We aim to evaluate the efficacy of two different arterial ligation techniques in controlling postpartum haemorrhage and minimizing bleeding complications. We searched six databases. 11 studies were finally included into our review and analysis. We graded their quality using the Cochrane tool for randomized trials and the NIH tool for retrospective studies. Our analysis showed that internal iliac artery ligation has no significant effect on bleeding control (MD = −248.60 [−1045.55, 548.35] P = 0.54), while uterine artery ligation significantly reduced the amount of blood loss and preserved the uterus (MD = −260.75, 95% CI [−333.64, −187.86], P < 0.00001). Uterine artery ligation also minimized the need for blood transfusion. Bleeding was best controlled by combining both uterine artery ligation with uterine tamponade (MD = 1694.06 [1675.34, 1712.78], P < 0.00001). This combination also showed a significant decrease in hysterectomy compared to the uterine artery ligation technique alone. Bilateral uterine artery ligation in women with placenta accreta spectrum can effectively reduce the amount of bleeding and the risk of complications. The best bleeding control tested is a combination of both, uterine artery ligation and cervical tamponade. These techniques may offer an easy and applicable way to preserve fertility in PAS patients. Larger randomized trials are needed to define the best technique.
Use of new approach methodologies (NAMs) to meet regulatory requirements for the assessment of industrial chemicals and pesticides for effects on human health
Authors: Stucki Andreas O. , Barton-Maclaren Tara S. , Bhuller Yadvinder , Henriquez Joseph E. , Henry Tala R. , Hirn Carole , Miller-Holt Jacqueline , Nagy Edith G. , Perron Monique M. , Ratzlaff Deborah E. , Stedeford Todd J. , Clippinger Amy J.
Published: September 2022

New approach methodologies (NAMs) are increasingly being used for regulatory decision making by agencies worldwide because of their potential to reliably and efficiently produce information that is fit for purpose while reducing animal use. This article summarizes the ability to use NAMs for the assessment of human health effects of industrial chemicals and pesticides within the United States, Canada, and European Union regulatory frameworks. While all regulations include some flexibility to allow for the use of NAMs, the implementation of this flexibility varies across product type and regulatory scheme. This article provides an overview of various agencies’ guidelines and strategic plans on the use of NAMs, and specific examples of the successful application of NAMs to meet regulatory requirements. It also summarizes intra- and inter-agency collaborations that strengthen scientific, regulatory, and public confidence in NAMs, thereby fostering their global use as reliable and relevant tools for toxicological evaluations. Ultimately, understanding the current regulatory landscape helps inform the scientific community on the steps needed to further advance timely uptake of approaches that best protect human health and the environment.
A Rotary-Linear Ultrasonic Motor Using MnO2-Doped (Ba0.97Ca0.03)(Ti0.96Sn0.005Hf0.035)O3 Lead-Free Piezoelectric Ceramics with Improved Curie Temperature and Temperature Stability
Authors: Tsai Cheng-Che , Chu Sheng-Yuan , Chao Wei-Hsiang , Hong Cheng-Shong
Published: September 2022

In this work, a cylindrical lead-free rotary-linear ultrasonic motor was attached to piezoelectric plates of MnO2-doped (Ba0.97Ca0.03)(Ti0.96Sn0.005Hf0.035)O3 ceramics using the first bending vibration to pull a thread output shaft of the interior of a stator. The effect of the proposed ceramics’ d33 and Qm values are the key factors for ultrasonic motors. Therefore, MnO2-doped (Ba0.97Ca0.03)(Ti0.96Sn0.005Hf0.035)O3 lead-free piezoelectric ceramics with high values of d33 = 230 pC/N, Qm = 340.8 and a good temperature stability of their dielectric and piezoelectric properties are suitable for application to linear piezoelectric motors. The structure of the linear piezoelectric motor was simulated and fabricated by Finite Element Analysis. The characteristics of linear piezoelectric motors were also studied. The output characteristics of the lead-free piezoelectric motor were a left-pull velocity = 3.21 mm/s, a right-pull velocity = 3.39 mm/s, an up-pull velocity = 2.56 mm/s and a force >2 N at 39.09 kHz for an input voltage of approximately 200 Vp-p (peak to peak). These results are comparable to those for a lead-based piezoelectric motor that uses PZT-4 ceramics. The proposed lead-free piezoelectric motors were successfully fabricated and used to pull a 0.5 mL commercial insulin syringe.
Machine Learning for Detection and Prediction of Crop Diseases and Pests: A Comprehensive Survey
Authors: Domingues Tiago , Brandão Tomás , Ferreira João C.
Published: September 2022

Considering the population growth rate of recent years, a doubling of the current worldwide crop productivity is expected to be needed by 2050. Pests and diseases are a major obstacle to achieving this productivity outcome. Therefore, it is very important to develop efficient methods for the automatic detection, identification, and prediction of pests and diseases in agricultural crops. To perform such automation, Machine Learning (ML) techniques can be used to derive knowledge and relationships from the data that is being worked on. This paper presents a literature review on ML techniques used in the agricultural sector, focusing on the tasks of classification, detection, and prediction of diseases and pests, with an emphasis on tomato crops. This survey aims to contribute to the development of smart farming and precision agriculture by promoting the development of techniques that will allow farmers to decrease the use of pesticides and chemicals while preserving and improving their crop quality and production.
Heat Shock Treatment Promoted Callus Formation on Postharvest Sweet Potato by Adjusting Active Oxygen and Phenylpropanoid Metabolism
Authors: Xin Qi , Liu Bangdi , Sun Jing , Fan Xinguang , Li Xiangxin , Jiang Lihua , Hao Guangfei , Pei Haisheng , Zhou Xinqun
Published: September 2022

This study aimed to investigate that rapid high-temperature treatment (RHT) at an appropriate temperature could accelerate callus formation by effectively promoting the necessary metabolic pathways in sweet potato callus. In this study, the callus of sweet potato was treated with heat shock at 50, 65, and 80 °C for 15 min. The callus formation was observed within 1, 3, and 5 days, and the accumulation of intermediates in the metabolism of phenylpropane and reactive oxygen species and changes in enzyme activities were determined. The results showed that appropriate RHT treatment at 65 °C stimulated the metabolism of reactive oxygen species at the injury site of sweet potato on the first day, and maintained a high level of reactive oxygen species production and scavenging within 5 days. The higher level of reactive oxygen species stimulated the phenylalanine ammonia-lyase (PAL), 4-coumarate-CoA ligase and cinnamate-4-hydroxylase activities of the phenylpropane metabolic pathway, and promoted the rapid synthesis of chlorogenic acid, p-coumaric acid, rutin, and caffeic acid at the injury site, which stacked to form callus. By Pearson’s correlation analysis, catalase (CAT), PAL, and chlorogenic acid content were found to be strongly positively correlated with changes in all metabolites and enzymatic activities. Our results indicated that appropriate high-temperature rapid treatment could promote sweet potato callus by inducing reactive oxygen species and phenylpropane metabolism; moreover, CAT, PAL, and chlorogenic acid were key factors in promoting two metabolic pathways in sweet potato callus.
Soil Organic Nitrogen Indirectly Enhances Pepper-Residue-Mediated Soil Disease Suppression through Manipulation of Soil Microbiome
Authors: Hong Shan , Jv Hongling , Yuan Xianfu , Geng Jianjian , Wang Beibei , Zhao Yan , Wang Qing , Li Rong , Jia Zhongjun , Ruan Yunze
Published: September 2022

Banana Fusarium wilt-suppressive soils are effective against pathogen invasion, yet soil physicochemical factors responsible for conducive or suppressive behavior have not been reported. Here, we investigated the changes in banana biomass, disease incidence (DI), soil culturable microbes and physicochemical properties by incorporating pepper and banana residues into conducive and suppressive soils. Before the incorporation of any residues, the suppressive soil significantly increased banana biomass and decreased DI compared to the conducive soil. The biomass of the suppressive soil was significantly higher than that of the conducive soil after the incorporation of either pepper or banana residues. Compared with the control (CK), the incorporation of pepper residues to both soils significantly reduced DI, while banana residues had the opposite effect. Additionally, both conducive and suppressive soils supplemented with pepper residues significantly reduced the amounts of culturable Fusarium oxysporum and increased the amounts of beneficial Pseudomonas and Bacillus. The pepper residue extracts significantly inhibited the growth of F. oxysporum mycelium. Soil alkali-hydrolyzable nitrogen (AN) responded most strongly to residue application to suppressive soil. The AN factor was significantly and positively correlated with banana biomass; however, there was no direct and significant negative correlation with DI. Further analysis of the results showed that elevated AN content could stimulate the amounts of culturable Bacillus in the soil, and Bacillus antagonized the proliferation of pathogen and thus indirectly and effectively reduced banana DI. In conclusion, soil AN content can indirectly improve the disease suppression ability of pepper-residue-mediated suppressive soil by manipulating the soil microbiome.
Texture and Digestion Properties Based on Amylose Content and Gel Consistency in Landraces and Recently-Released Cultivars of Indica Rice in China
Authors: Huang Min , Xie Jiaxin , Chen Jiaxin , Zhao Chunrong , Liao Chengjing , Li Beilei , Shu Ao , Chen Jiana , Cao Fangbo
Published: September 2022

China has abundant rice landrace resources and has developed many new rice cultivars over the past several decades. To identify the differences in texture and digestion properties between landraces and recently-released cultivars of indica rice in China, this study estimated the hardness (Hd) and starch digestion rate (SDR) of cooked rice based on the amylose content (AC) and gel consistency (GC) of landraces and recently-released (2019–2021) cultivars of indica rice in eight provinces in southern China. The results demonstrated that AC ranged from 11.3% to 34.0% in landraces and from 10.0% to 28.6% in recently-released cultivars. The AC median was lower in recently-released cultivars than in landraces by approximately 40%. GC ranged from 21–100 mm in landraces and from 30–95 mm in recently-released cultivars. The GC median was about 130% higher in recently-released cultivars compared to landraces. Hd ranged from 720–1418 g in landraces and from 590–1244 g in recently-released cultivars. The Hd median was approximately 35% lower in recently-released cultivars compared to landraces. SDR ranged from 1.04–2.86 mg g min−1 in landraces and from 1.71–3.37 mg g min−1 in recently-released cultivars. The SDR median was higher in recently-released cultivars than in landraces by about 60%. The findings of this study suggest that recently-developed indica rice cultivars in China can cater to the preference of consumers for soft-texture rice but increase health risks due to a higher rate of digesting starch into glucose.
Policy Gaps Related to Sustainability in Hungarian Agribusiness Development
Authors: Hoyk Edit , Szalai Ádám , Palkovics András , Farkas Jenő Zsolt
Published: September 2022

The world’s agriculture faces many challenges nowadays, such as tackling the effects of climate change, conserving agrobiodiversity, or feeding the Earth’s growing population. These issues often induce conflicting development directions, such as digitalization and ecologization, as the case of the European Union’s Common Agricultural Policy (CAP) shows. In the last decades, policymakers have focused mainly on greening agricultural production and the food industry, and now the CAP is part of the European Green Deal. In our research, we assessed the sustainability problems affecting the agribusiness sector and food consumption in Hungary using descriptive statistical analysis. On the other hand, we examined the latest sectoral development documents (Digital Agricultural Strategy, Digital Food Industry Strategy) in order to find out to what extent they answer the identified issues. Our results revealed that the Hungarian agribusiness sector is struggling with several sustainability challenges, which do not receive adequate attention from policymakers. The newest development strategies are characterized by forced digitalization efforts, while their applicability and effectiveness are uncertain. Because of similar development trajectories, we believe most of our results are relevant to other Central Eastern European Member states. Hence, further CAP and national policy reforms are needed to make Europe’s agribusiness sector more sustainable.
Benefits and Conflicts: A Systematic Review of Dog Park Design and Management Strategies
Authors: Chen Shuolei , Wu Zhuoran , Sleipness Ole Russell , Wang Hao
Published: September 2022

Dog ownership and dog walking brings various health benefits for urban dwellers, especially since the COVID-19 pandemic, but trigger a number of controversies. Dog parks have become increasingly significant public resources in the pandemic to support these benefits while facing intense conflicts. To develop effective dog parks in urban settings, growing numbers of scholars have provided insights into the design and management strategies for addressing the benefits and conflicts. The objective of this study is to synthesize and analyze various aspects of dog park design and management and to assess identified strategies for enhancing their benefits while mitigating their drawbacks. Following the PRISMA guidelines, a systematic study was conducted to synthesize the benefits, conflicts, and management strategies of dog parks, supported by Citespace. Benefits and conflicts in dog park design and management have been synthesized and organized according to their frequency of presence and the statistical results. We analyzed and assessed existing design and management strategies. Through this systematic study, we discovered the need obtain o po experimental evidence on effective dog park design and management to enhance their benefits while mitigating their sources of conflict and limitations in the intensity of park visitors’ physical activity in off-leash areas. Guidelines for the design and management strategies for effective dog parks were made to enhance their benefits while alleviating conflicts in the future development of sustainable dog parks that promote healthy relationships between canines and residents in urban built environments.
European Regulations on Camel Germplasm Movement within the European Union: A Current Framework Based on Safety
Authors: Zema Elena , Monti Salvatore , Biondi Vito , Faraz Asim , Pugliese Michela , Marino Gabriele , Passantino Annamaria
Published: September 2022

With the aim of developing livestock breeding, the Delegated Regulation (EU) 2020/686 (hereafter referred to as Reg. 686) has taken steps to define traceability and animal health for the movement of germ material within the European Union (EU), including that of camelid species. Despite the economic importance of the camel market and the efforts of the EU to regulate their movements, there are considerable difficulties in the collection of semen and its freezing, limiting the use of artificial insemination in this species. If, on the one hand, there is little diffusion of the camel breeding and, consequently, limited diffusion of animals and germplasm, there will probably be a significant increase over the years. To avoid the spread of emerging diseases—or even those no longer present in Europe—the entry of genetic material from non-EU countries must be strictly monitored. Camels are rarely clinically compliant, but can transfer even fatal diseases to domestic ungulate farms in the EU. Based on these considerations, we conducted a narrative review of the European regulations on this issue, focusing on aspects related to their application in camels.
Importance of the Lunar Cycle on Mesopelagic Foraging by Atlantic Bluefin Tuna in the Upwelling Area of the Strait of Messina (Central Mediterranean Sea)
Authors: Battaglia Pietro , Pedà Cristina , Malara Danilo , Milisenda Giacomo , MacKenzie Brian R. , Esposito Valentina , Consoli Pierpaolo , Vicchio Teresa Manuela , Stipa Maria Giulia , Pagano Luca , Longo Francesco , Romeo Teresa
Published: September 2022

The influence of the lunar cycle on bluefin tuna foraging in the upwelling area of the Strait of Messina was investigated by exploring trophic interaction with mesopelagic fish and cephalopod prey. To focus on how the lunar cycle could affect availability of mesopelagic prey for this predator, we tested potential differences in the diet related to each lunar phase. Moreover, we considered two potential impacts of the lunar cycle: the lunar irradiance and the strength of currents. Overall, 2672 prey items were mesopelagic fish and cephalopods, representing 60.7% of overall diet by number. The main mesopelagic fish prey items were lanternfishes and dragonfishes, while Onychoteuthis banksii was the most important cephalopod prey. In summary, the Strait of Messina has highly specific hydrodynamic and biological features which strongly depend on upwelling currents, which in turn are influenced by the lunar cycle (new and full moon with strong currents, quarters with fewer currents). Upwelling causes water mixing, bringing to the surface a large amount of mesopelagic fauna which become more readily available to tuna. Lunar irradiance contributes to the variation of prey composition, increasing the success of visual predation on mesopelagic resources at high light in the water column.
Perspectives of Immigrants and Native Dutch on Antibiotic Use: A Qualitative Study
Authors: Lescure Dominique L. A. , van der Velden Alike W. , Huijser van Reenen Natascha , Richardus Jan Hendrik , Voeten Helene A. C. M.
Published: September 2022

Immigrants constitute large proportions of the population in many high-income countries. Knowledge about their perceptions of antibiotics, in comparison to native populations, is limited. We explored these perceptions by organizing nine homogeneous focus group discussions (FGDs) with first-generation immigrant and native Dutch participants (N = 64) from Rotterdam and Utrecht, who were recruited with the assistance of immigrant (community support) organizations. The FGDs were audio-recorded and transcribed verbatim. Inductive thematic analyses were performed with the qualitative analysis software Atlas.ti, using open and axial coding. We did not find noteworthy differences between immigrants and native Dutch participants; all participants had an overall reluctant attitude towards antibiotics. Within-group differences were larger than between-group differences. In each FGD there were, for instance, participants who adopted an assertive stance in order to receive antibiotics, who had low antibiotic-related knowledge, or who used antibiotics incorrectly. Native Dutch participants expressed similar difficulties as immigrant participants in the communication with their GP, which mainly related to time constraints. Immigrants who encountered language barriers experienced even greater communicational difficulties and reported that they often feel embarrassed and refrain from asking questions. To stimulate more prudent use of antibiotics, more attention is needed for supportive multilingual patient materials. In addition, GPs need to adjust their information, guidance, and communication for the individual’s needs, regardless of the patient’s migration background.
Mixing Propolis from Different Apiaries and Harvesting Years: Towards Propolis Standardization?
Authors: Peixoto Marta , Freitas Ana Sofia , Cunha Ana , Oliveira Rui , Almeida-Aguiar Cristina
Published: September 2022

Global demand for safe, effective and natural products has been increasing in parallel with consumers’ concerns about personal and environmental health. Propolis, a traditional and potentially medicinal product with several health benefits, is a beehive product with a worldwide reputation. However, despite the bioactivities reported, the low productivity and high chemical heterogeneity have been extensively hampering broader industrial uses. To assist in overcoming some of these problems, we prepared and characterized mixtures of ethanol extracts of a heterogeneous propolis sample (Pereiro) collected over a five-year period (2011–2015) and, additionally, we mixed two different propolis samples from distinct regions of Portugal (Pereiro and Gerês), also harvested at different times. An investigation of the antimicrobial and antioxidant properties, as well as characterization of the chemical composition of the eleven propolis blends were performed in this work. The antioxidant and antimicrobial activities of such blends of propolis samples, either from different localities and/or different years, were maintained, or even enhanced, when a comparison of the individual extracts was conducted. The differences in the chemical composition of the original propolis samples were also diluted in the mixtures. The results reemphasize the great potential of propolis and suggest that mixing different samples, regardless of provenance or harvesting date, can contribute to propolis standardization while simultaneously increasing its availability and adding value to this beehive byproduct.
Oxidative DNA Damage in the Pathophysiology of Spinal Cord Injury: Seems Obvious, but Where Is the Evidence?
Authors: Scheijen Elle E. M. , Hendrix Sven , Wilson David M.
Published: September 2022

Oxidative stress occurs at various phases of spinal cord injury (SCI), promoting detrimental processes such as free radical injury of proteins, nucleic acids, lipids, cytoskeleton, and organelles. Oxidative DNA damage is likely a major contributor to the pathogenesis of SCI, as a damaged genome cannot be simply turned over to avert detrimental molecular and cellular outcomes, most notably cell death. Surprisingly, the evidence to support this hypothesis is limited. There is some evidence that oxidative DNA damage is increased following SCI, mainly using comet assays and immunohistochemistry. However, there is great variability in the timing and magnitude of its appearance, likely due to differences in experimental models, measurement techniques, and the rigor of the approach. Evidence indicates that 8-oxodG is most abundant at 1 and 7 days post-injury (dpi), while DNA strand breaks peak at 7 and 28 dpi. The DNA damage response seems to be characterized by upregulation of PCNA and PARP1 but downregulation of APEX1. Significant improvements in the analysis of oxidative DNA damage and repair after SCI, including single-cell analysis at time points representative for each phase post-injury using new methodologies and better reporting, will uncover the role of DNA damage and repair in SCI.
Recombinant Expression of Archaeal Superoxide Dismutases in Plant Cell Cultures: A Sustainable Solution with Potential Application in the Food Industry
Authors: Gogliettino Marta , Arciello Stefania , Cillo Fabrizio , Carluccio Anna Vittoria , Palmieri Gianna , Apone Fabio , Ambrosio Rosa Luisa , Anastasio Aniello , Gratino Lorena , Carola Antonietta , Cocca Ennio
Published: September 2022

Superoxide dismutase (SOD) is a fundamental antioxidant enzyme that neutralises superoxide ions, one of the main reactive oxygen species (ROS). Extremophile organisms possess enzymes that offer high stability and catalytic performances under a wide range of conditions, thus representing an exceptional source of biocatalysts useful for industrial processes. In this study, SODs from the thermo-halophilic Aeropyrum pernix (SODAp) and the thermo-acidophilic Saccharolobus solfataricus (SODSs) were heterologously expressed in transgenic tomato cell cultures. Cell extracts enriched with SODAp and SODSs showed a remarkable resistance to salt and low pHs, respectively, together with optimal activity at high temperatures. Moreover, the treatment of tuna fillets with SODAp-extracts induced an extension of the shelf-life of this product without resorting to the use of illicit substances. The results suggested that the recombinant plant extracts enriched with the extremozymes could find potential applications as dietary supplements in the nutrition sector or as additives in the food preservation area, representing a more natural and appealing alternative to chemical preservatives for the market.
Effects of Thermally-Oxidized Frying Oils (Corn Oil and Lard) on Gut Microbiota in Hamsters
Authors: Kwek Erika , Yan Chi , Ding Huafang , Hao Wangjun , He Zouyan , Ma Ka Ying , Liu Jianhui , Zhu Hanyue , Chen Zhen-Yu
Published: September 2022

Repeated reuse of frying oil raises health concerns due to the accumulation of oxidative products after each frying cycle. Gut microbiota is integral in lipid metabolism and immune regulation. The present study was designed to investigate the effects of thermally-oxidized corn oil and lard on gut microbiota in relation to atherosclerosis, inflammatory cytokines, and plasma lipids. Male Golden Syrian hamsters were randomly divided into four groups and fed one of four diets containing fresh corn oil (CF), oxidized corn oil (CO), fresh lard (LF), and oxidized lard (LO), for six weeks. CO and LO were prepared by deep-frying potatoes in corn oil or lard for seven days. Results indicated that oxidized oil and lard caused the loss of species diversity and richness of gut microbiota. Feeding CO and LO also reduced the body and adipose tissue weights, associated with genus Acetatifactor and Allobaculum. Plasma triacylglycerols significantly increased by 51% in the CO and 35% in the LO group compared with that in their CF and LF counterparts, respectively. CO could also affect the abundance of specific bacteria genera: Bacteroides, Barnesiella, Acetatifactor, Allobaculum, Clostridium_IV, Clostridium_XIVa, Coprococcus, Lactococcus, Paraprevotella, Parasutterella, and Oscillibacter. In addition, CO and LO could adversely remodel gut composition and affect intestinal production of short-chain fatty acids, pro-inflammatory biomarkers (LPS and IL-6), anti-inflammatory biomarker IL-10, and atherosclerotic progression. It was concluded that frying oil could adversely modulate the gut microbiota and exacerbate the atherosclerosis at least in a hypercholesterolemia hamster model.
The Inhibitory Effect of Quercetin on Biofilm Formation of Listeria monocytogenes Mixed Culture and Repression of Virulence
Authors: Roy Pantu Kumar , Song Min Gyu , Park Shin Young
Published: September 2022

Listeria monocytogenes is the species of foodborne pathogenic bacteria that causes the infection listeriosis. The food production chain employs various methods to control biofilms, although none are completely successful. This study evaluates the effectiveness of quercetin as a food additive in reducing L. monocytogenes mixed cultures (ATCC19113, ATCC19117, and ATCC15313) biofilm formation on stainless steel (SS), silicon rubber (SR), and hand glove (HG) coupons, as well as tests its antimicrobial activities. With a minimum inhibitory concentration (MIC) of 250 µg/mL, the tested quercetin exhibited the lowest bactericidal action with no visible bacterial growth. In contrast, during various experiments in this work, the inhibitory efficacy of quercetin at sub-MICs levels (1/2, 1/4, and 1/8 MIC) against L. monocytogenes was examined. A control group was not added with quercetin. The current study also investigates the effect of quercetin on the expression of different genes engaged in motility (flaA, fbp), QS (agrA), and virulence (hlyA, prfA). Through increasing quercetin concentration, swarming and swimming motility, biofilm formation, and expression levels of target genes linked to flagella motility, virulence, and quorum-sensing were all dramatically reduced. Quercetin (0–125 μg/mL) was investigated on the SS, SR, and HG surfaces; the inhibitory effects were 0.39–2.07, 0.09–1.96 and 0.03–1.69 log CFU/cm2, respectively (p < 0.05). Field-emission scanning electron microscopy (FE-SEM) corroborated the findings because quercetin prevented the development of biofilms by severing cell-to-cell contacts and inducing cell lysis, which resulted in the loss of normal cell shape. Our findings suggest that plant-derived quercetin should be used as an antimicrobial agent in the food industry to control the development of L. monocytogenes biofilms. These outcomes suggest that bacterial targets are of interest for biofilm reduction, with alternative natural food agents in the food sector along the entire food production chain.
Integrin-Linked Kinase Expression in Human Valve Endothelial Cells Plays a Protective Role in Calcific Aortic Valve Disease
Authors: Sánchez-Esteban Sandra , Castro-Pinto Mercedes , Cook-Calvete Alberto , Reventún Paula , Delgado-Marín María , Benito-Manzanaro Lucía , Hernandez Ignacio , López-Menendez José , Zamorano José Luis , Zaragoza Carlos , Saura Marta
Published: September 2022

Calcific aortic valve disease (CAVD) is highly prevalent during aging. CAVD initiates with endothelial dysfunction, leading to lipid accumulation, inflammation, and osteogenic transformation. Integrin-linked kinase (ILK) participates in the progression of cardiovascular diseases, such as endothelial dysfunction and atherosclerosis. However, ILK role in CAVD is unknown. First, we determined that ILK expression is downregulated in aortic valves from patients with CAVD compared to non-CAVD, especially at the valve endothelium, and negatively correlated with calcification markers. Silencing ILK expression in human valve endothelial cells (siILK-hVECs) induced endothelial-to-mesenchymal transition (EndMT) and promoted a switch to an osteoblastic phenotype; SiILK-hVECs expressed increased RUNX2 and developed calcified nodules. siILK-hVECs exhibited decreased NO production and increased nitrosative stress, suggesting valvular endothelial dysfunction. NO treatment of siILK-hVECs prevented VEC transdifferentiation, while treatment with an eNOS inhibitor mimicked ILK-silencing induction of EndMT. Accordingly, NO treatment inhibited VEC calcification. Mechanistically, siILK-hVECs showed increased Smad2 phosphorylation, suggesting a TGF-β-dependent mechanism, and NO treatment decreased Smad2 activation and RUNX2. Experiments performed in eNOS KO mice confirmed the involvement of the ILK-eNOS signaling pathway in valve calcification, since aortic valves from these animals showed decreased ILK expression, increased RUNX2, and calcification. Our study demonstrated that ILK endothelial expression participates in human CAVD development by preventing endothelial osteogenic transformation.
Digital Watermarking System for Copyright Protection and Authentication of Images Using Cryptographic Techniques
Authors: Sanivarapu Prasanth Vaidya , Rajesh Kandala N. V. P. S. , Hosny Khalid M. , Fouda Mostafa M.
Published: September 2022

Digital images are transferred with ease through the network. Many users are using the images without the knowledge of the owners. Therefore, a novel watermarking scheme is proposed to ensure copyright protection and authentication of images using cryptography techniques. Here, a quick response (QR) image is generated for a watermark image that contains public and private keys prepared using a cryptosystem. Later, this QR image is scrambled using a chaotic logistic map. The public and private keys are used to cipher and decipher the data. Next, the scrambled QR watermark is embedded into a color image using a single-level discrete wavelet transform followed by singular value decomposition using the key value. Finally, the inverse process is applied to extract the watermark. The proposed method is validated using various image processing attacks. The results are then compared with state-of-the-art watermarking schemes. The experimental results show that the scheme provides good results in terms of robustness and imperceptibility.
A Feasibility Study for the Prediction of Concrete Pavement Condition Index (CPCI) Based on Machine Learning
Authors: Lee Jin-Hyuk , Jung Dong-Hyuk , Lee Moon-Sub , Jeon Sung-Il
Published: September 2022

In South Korea, various attempts have been made to utilize the Pavement Management System database (PMS DB) more efficiently as a basis for preventive maintenance. Data for the PMS DB is extensively collected every year. This study aims to predict future pavement conditions by introducing the concept of machine learning instead of regression modeling. We selected 469 sections that satisfied the analysis conditions and used them for analysis. We used particle filtering, a machine learning technique, to predict future pavement conditions. We found that the function of the particle filtering technique itself increases the prediction accuracy for the target section analyzed as the number of particles increases. Furthermore, the number of time series points in one section had a higher impact on the improvement of prediction accuracy than the number of sections analyzed. Finally, the relative error by each predicted age for the same section decreased as the number of time series points increased. These findings indicate that the rate of decrease in the performance index can be quantitatively presented in the future, and the method developed in this study could be used by pavement managers during the decision-making process.
Antimicrobial Resistance Distribution and Quorum-Sensing Regulation of Enterococcal Strains, Isolated from Hospitalized Patients
Authors: Yordanova Rozalina , Yaneva Zvezdelina , Gencheva Deyana , Beev Georgi
Published: September 2022

Background: Enterococci are intrinsically resistant/tolerant to various antimicrobial agents and can also acquire and combine different mechanisms of resistance, including quorum-sensing regulation, to most active compounds, which makes enterococcal infection treatment even more challenging. The aim of this study was to evaluate the pattern of antimicrobial resistance and to analyze the frequency of quorum-sensing asa1 and esp genes in clinical isolates representing the genus Enterococcus. Methods: Multiplex PCR assays were performed for the identification of 110 enterococcal isolates and the determination of their antibiotic susceptibility and the presence of asa1/esp genes. Additionally, the antibiotic resistance of the isolates was tested by the Kirby–Bauer disk diffusion method. Results: 90% of the isolates were identified as Enterococcus faecalis and 10% as Enterococcus faecium. Quorum-sensing regulation genes were present in 109 isolates. Aminoglycoside (aac(6′)/aph(2″)-, quinolone (emeA)-, β-lactams (TEM)-, and vancomycin (vanA)-resistance genes were detected in 108 isolates. All of the isolates tested were vanB negative. According to the Kirby–Bauer method, 39% of the isolates expressed multidrug resistance (MDR) and 33% of the MDR E. faecium were vancomycin-resistant. Conclusion: The large percentage of MDR enterococci possessing asa1/esp genes indicated a possible connection between quorum-sensing regulation and drug resistance. Therefore, the regular monitoring of the antimicrobial resistance of Enterococcus spp., and the identification of virulence factors are needed. It is also important to prevent host colonization through the elimination of factors leading to the expression of quorum-sensing genes.
Unsupervised and Supervised Feature Selection for Incomplete Data via L2,1-Norm and Reconstruction Error Minimization
Authors: Cai Jun , Fan Linge , Xu Xin , Wu Xinrong
Published: September 2022

Feature selection has been widely used in machine learning and data mining since it can alleviate the burden of the so-called curse of dimensionality of high-dimensional data. However, in previous works, researchers have designed feature selection methods with the assumption that all the information from a data set can be observed. In this paper, we propose unsupervised and supervised feature selection methods for use with incomplete data, further introducing an L2,1 norm and a reconstruction error minimization method. Specifically, the proposed feature selection objective functions take advantage of an indicator matrix reflecting unobserved information in incomplete data sets, and we present pairwise constraints, minimizing the L2,1-norm-robust loss functionand performing error reconstruction simultaneously. Furthermore, we derive two alternative iterative algorithms to effectively optimize the proposed objective functions and the convergence of the proposed algorithms is proven theoretically. Extensive experimental studies were performed on both real and synthetic incomplete data sets to demonstrate the performance of the proposed methods.