bluuebunny/crossref_metadata_2025 huggingface dataset:
Association between gut microbiota and gastric cancers: a two-sample Mendelian randomization study
Authors: Chang Yuan , Gao Guanzhuang , Feng Cuncheng
Published: April 2024

Background

Gastric cancer (GC) is the fifth most commonly diagnosed cancer worldwide, with its etiology attributed to a complex interplay of genetic, dietary, environmental factors, and infections such as Helicobacter pylori. Despite the known risk factors, the role of gut microbiota in the development of gastric cancer remains insufficiently explored. This study aims to elucidate the causal relationship between gut microbiota and gastric cancer using a two-sample Mendelian Randomization (MR) approach.

Methods

Utilizing genome-wide association study (GWAS) summary data from the MiBioGen consortium and gastric cancer datasets, we selected instrumental variables for MR analysis based on their association with specific microbiota. We employed several MR methods, including inverse variance weighted (IVW), MR-Egger, weighted median, and others, to estimate the causal effects of gut microbiota diversity on the risk of developing gastric cancer.

Results

Our analysis identified significant associations between certain gut microbiota and gastric cancer risk. Specifically, taxa such as Clostridium sensustricto1 (OR = 0.540, 95%CI: 0.354–0.823, p = 0.004), Actinomycetales (OR = 0.756, 95%CI: 0.613–0.932, p = 0.009), Selenomonadales (OR = 0.816, 95%CI: 0.666–1.000, p < 0.05), Negativicutes (OR = 0.816, 95%CI: 0.666–1.000, p < 0.05), Rikenellaceae (OR = 0.863, 95%CI: 0.746–0.999, p = 0.048) were found to have a protective effect against gastric cancer. Conversely, an increased risk of gastric cancer was associated with the abundance of Roseburia (OR = 1.342, 95%CI: 1.071–1.681, p = 0.011), Family XI (OR = 1.132, 95%CI: 1.012–1.267, p = 0.030), and Eubacterium brachy group (OR = 1.207, 95%CI: 1.074–1.355, p = 0.002). The findings were robust across various MR methods and were not driven by any single SNP, indicating a genuine causal relationship.

Conclusion

Our studies have shown that there is a causal relationship between intestinal flora and gastric cancer at the genetic level. Clostridium sensustricto1, Actinomycetales, Rikenellaceae, Selenomonadales, Negativicutes, and Actinomycetaceae as having a protective role against GC, while Roseburia, Family XI, and Eubacterium brachy group were associated with an increased risk.
The Radiation Therapy Technology Evidence Matrix: a framework to visualize evidence development for innovations in radiation therapy
Authors: Edwards Sarah , Luzzara Marco , Dell’Acqua Veronica , Christodouleas John
Published: April 2024

Clinical evidence is crucial in enabling the judicious adoption of technological innovations in radiation therapy (RT). Pharmaceutical evidence development frameworks are not useful for understanding how technical advances are maturing. In this paper, we introduce a new framework, the Radiation Therapy Technology Evidence Matrix (rtTEM), that helps visualize how the clinical evidence supporting new technologies is developing. The matrix is a unique 2D model based on the R-IDEAL clinical evaluation framework. It can be applied to clinical hypothesis testing trials, as well as publications reporting clinical treatment. We present the rtTEM and illustrate its application, using emerging and mature RT technologies as examples. The model breaks down the type of claim along the vertical axis and the strength of the evidence for that claim on the horizontal axis, both of which are inherent in clinical hypothesis testing. This simplified view allows for stakeholders to understand where the evidence is and where it is heading. Ultimately, the value of an innovation is typically demonstrated through superiority studies, which we have divided into three key categories – administrative, toxicity and control, to enable more detailed visibility of evidence development in that claim area. We propose the rtTEM can be used to track evidence development for new interventions in RT. We believe it will enable researchers and sponsors to identify gaps in evidence and to further direct evidence development. Thus, by highlighting evidence looked for by key policy decision makers, the rtTEM will support wider, timely patient access to high value technological advances.
Natural products targeting autophagy and apoptosis in NSCLC: a novel therapeutic strategy
Authors: Qin Peiyi , Li Qingchen , Zu Qi , Dong Ruxue , Qi Yuanfu
Published: April 2024

Lung cancer is the leading cause of cancer-related mortality worldwide, with non-small cell lung cancer (NSCLC) being the predominant type. The roles of autophagy and apoptosis in NSCLC present a dual and intricate nature. Additionally, autophagy and apoptosis interconnect through diverse crosstalk molecules. Owing to their multitargeting nature, safety, and efficacy, natural products have emerged as principal sources for NSCLC therapeutic candidates. This review begins with an exploration of the mechanisms of autophagy and apoptosis, proceeds to examine the crosstalk molecules between these processes, and outlines their implications and interactions in NSCLC. Finally, the paper reviews natural products that have been intensively studied against NSCLC targeting autophagy and apoptosis, and summarizes in detail the four most retrieved representative drugs. This paper clarifies good therapeutic effects of natural products in NSCLC by targeting autophagy and apoptosis and aims to promote greater consideration by researchers of natural products as candidates for anti-NSCLC drug discovery.
Hypothermia amongst neonatal admissions in Kenya: a retrospective cohort study assessing prevalence, trends, associated factors, and its relationship with all-cause neonatal mortality
Authors: Wainaina John , Ogero Morris , Mumelo Livingstone , Wairoto Kefa , Mbevi George , Tuti Timothy , Mwaniki Paul , Irimu Grace , English Mike , Aluvaala Jalemba ,   The Clinical Information Network Author Group
Published: April 2024

Background

Reports on hypothermia from high-burden countries like Kenya amongst sick newborns often include few centers or relatively small sample sizes.

Objectives

This study endeavored to describe: (i) the burden of hypothermia on admission across 21 newborn units in Kenya, (ii) any trend in prevalence of hypothermia over time, (iii) factors associated with hypothermia at admission, and (iv) hypothermia's association with inpatient neonatal mortality.

Methods

A retrospective cohort study was conducted from January 2020 to March 2023, focusing on small and sick newborns admitted in 21 NBUs. The primary and secondary outcome measures were the prevalence of hypothermia at admission and mortality during the index admission, respectively. An ordinal logistic regression model was used to estimate the relationship between selected factors and the outcomes cold stress (36.0°C–36.4°C) and hypothermia (<36.0°C). Factors associated with neonatal mortality, including hypothermia defined as body temperature below 36.0°C, were also explored using logistic regression.

Results

A total of 58,804 newborns from newborn units in 21 study hospitals were included in the analysis. Out of these, 47,999 (82%) had their admission temperature recorded and 8,391 (17.5%) had hypothermia. Hypothermia prevalence decreased over the study period while admission temperature documentation increased. Significant associations were found between low birthweight and very low (0–3) APGAR scores with hypothermia at admission. Odds of hypothermia reduced as ambient temperature and month of participation in the Clinical Information Network (a collaborative learning health platform for healthcare improvement) increased. Hypothermia at admission was associated with 35% (OR 1.35, 95% CI 1.22, 1.50) increase in odds of neonatal inpatient death.

Conclusions

A substantial proportion of newborns are admitted with hypothermia, indicating a breakdown in warm chain protocols after birth and intra-hospital transport that increases odds of mortality. Urgent implementation of rigorous warm chain protocols, particularly for low-birth-weight babies, is crucial to protect these vulnerable newborns from the detrimental effects of hypothermia.
Integrated transcriptomics and metabolomics analyses of the effects of bagging treatment on carotenoid biosynthesis and regulation of Areca catechu L.
Authors: Zheng Xin , Huang Liyun , Fan Benyi , Peng Chunlin , Iqbal Amjad , Zhang Yujie , Chen Hongman , Ye Jianqiu , Yang Yaodong
Published: April 2024

Introduction

Fresh Aareca nut fruit for fresh fruit chewing commonly found in green or dark green hues. Despite its economic significance, there is currently insufficient research on the study of color and luster of areca. And the areca nut fruits after bagging showed obvious color change from green to tender yellow. In the study, we tried to explain this interesting variation in exocarp color.

Methods

Fruits were bagged (with a double-layered black interior and yellow exterior) 45 days after pollination and subsequently harvested 120 days after pollination. In this study, we examined the the chlorophyll and carotenoid content of pericarp exocarp, integrated transcriptomics and metabolomics to study the effects of bagging on the carotenoid pathway at the molecular level.

Results

It was found that the chlorophyll and carotenoid content of bagged areca nut (YP) exocarp was significantly reduced. A total of 21 differentially expressed metabolites (DEMs) and 1784 differentially expressed genes (DEGs) were screened by transcriptomics and metabolomics. Three key genes in the carotenoid biosynthesis pathway as candidate genes for qPCR validation by co-analysis, which suggested their role in the regulation of pathways related to crtB, crtZ and CYP707A.

Discussion

We described that light intensity may appear as a main factor influencing the noted shift from green to yellow and the ensuing reduction in carotenoid content after bagging.
A novel semi-dominant mutation in brassinosteroid signaling kinase1 increases stomatal density
Authors: Ando Eigo , Taki Kyomi , Suzuki Takamasa , Kinoshita Toshinori
Published: April 2024

Stomata play a pivotal role in balancing CO2 uptake for photosynthesis and water loss via transpiration. Thus, appropriate regulation of stomatal movement and its formation are crucial for plant growth and survival. Red and blue light induce phosphorylation of the C-terminal residue of the plasma membrane (PM) H+-ATPase, threonine, in guard cells, generating the driving force for stomatal opening. While significant progress has been made in understanding the regulatory mechanism of PM H+-ATPase in guard cells, the regulatory components for the phosphorylation of PM H+-ATPase have not been fully elucidated. Recently, we established a new immunohistochemical technique for detecting guard-cell PM H+-ATPase phosphorylation using leaves, which was expected to facilitate investigations with a single leaf. In this study, we applied the technique to genetic screening experiment to explore novel regulators for the phosphorylation of PM H+-ATPase in guard cells, as well as stomatal development. We successfully performed phenotyping using a single leaf. During the experiment, we identified a mutant exhibiting high stomatal density, jozetsu (jzt), named after a Japanese word meaning ‘talkative’. We found that a novel semi-dominant mutation in BRASSINOSTEROID SIGNALING KINASE1 (BSK1) is responsible for the phenotype in jzt mutant. The present results demonstrate that the new immunohistochemical technique has a wide range of applications, and the novel mutation would provide genetic tool to expand our understanding of plant development mediated by brassinosteroid signaling.
From emotional signals to symbols
Authors: Griebel Ulrike , Oller D. Kimbrough
Published: April 2024

The quest for the origins of language is a diverse enterprise, where research from a variety of disciplines brings area-specific ideas and area-specific terminology to bear. This variety often results in misunderstandings and misconceptions about communication in various species. In the present paper, we argue for focus on emotional systems as the primary motivators for social signals in animals in general. This focus can help resolve discrepancies of interpretation among different areas of inquiry and can illuminate distinctions among different social signals as well as their phylogenetic origins in animals and especially in humans. We advocate, following Jaak Panksepp, a view wherein the Seeking System, the endogenous tendency to search and explore, is the most fundamental emotional motivation. The Seeking System forms the basis for flexible, voluntary, and exploratory control of motor systems and makes much of learning possible. The relative lack of vocal learning and expression in nonhuman primates contrasted with extensive vocal learning and expression in humans began, we propose, with the evolution in ancient hominins of a necessary foundation for the many subsequent capabilities required for language. That foundation was, according to the reasoning, naturally selected in the form of neurological connections between the Seeking System and mechanisms of glottal/phonatory control. The new connections allowed ancient hominins to develop flexible, endogenous vocal fitness signals produced at very high rates and including large numbers of discrete syllables, recombinable to form syllable combinations with many prosodic variations. The increasing sociality of hominins supported evolution of massive expansion in the utilization of these flexible vocal forms to allow development of words and sentences.
Pre-service teachers’ altruistic motivation for choosing teaching as a career: where does it come from?
Authors: Li Zhuotong , Guo Wu Yuan
Published: April 2024

This study aims to examine the factors influencing pre-service teachers’ altruistic motivation for selecting their profession within a Chinese educational setting. Drawing on existing research, a three-element (social-cognitive, emotional, and realistic) model is integrated to explore how pre-service teachers’ altruistic motivations are formed and evolved. Utilizing this model, interview data from 18 students enrolled in the Chinese Free Teacher Education program were collected and analyzed by thematic analysis. The findings indicate that social-cognitive factors impact altruistic motivation through engagement with social issues and reflections on practical educational challenges. The emotional factor is manifested through the participants’ positive and negative emotions. The realistic factor comprises familial influences and personal career preferences, which play a role in the decision to pursue teaching as a lifelong vocation. This study proposes a structured and functional model that can serve as a foundation for future research into the development of altruistic motivation. It also offers insights into nurturing altruistic motivation among both pre-service and practicing teachers in their career decision-making process.
Numerosity comparison, cognitive strategies, and general cognitive functioning in older people
Authors: Słupczewski Jakub , Gut Małgorzata , Matulewski Jacek , Tarnowski Adam
Published: April 2024

Introduction

Studies have shown age-related differences in numerical cognition, for example, in the level of numerosity comparison ability. Moreover, some studies point out individual differences in the cognitive strategies employed during the performance of numerosity comparison tasks and reveal that they are related to the aging process. One probable cause of these differences is the level of cognitive functioning. The aim of our study was to determine the relationships among numerosity comparison ability, the cognitive strategies utilized in the performance of numerosity comparison tasks and the general cognitive functioning in older people.

Methods

Forty-seven elderly people participated in the study. The participants were examined using overall cognitive functioning scales and computerized numerosity comparison task.

Results

The results showed many correlations between the participants’ level of cognitive functioning and the percent of correct responses (PCR) and response time (RT) during numerosity comparison, as well as with the cognitive strategies applied by the participants. Task correctness was positively related to the level of performance in the attention and executive function tasks. In contrast, the long-term memory resources index and visuospatial skills level were negatively correlated with RT regarding numerosity comparison task performance. The level of long-term memory resources was also positively associated with the frequency of use of more complex cognitive strategies. Series of regression analyses showed that both the level of general cognitive functioning and the cognitive strategies employed by participants in numerosity comparison can explain 9–21 percent of the variance in the obtained results.

Discussion

In summary, these results showed significant relationships between the level of cognitive functioning and proficiency in numerosity comparison measured in older people. Moreover, it has been shown that cognitive resources level is related to the strategies utilized by older people, which indicates the potential application for cognitive strategy examinations in the development of new diagnostic tools.
Using a music microanalysis protocol to enhance instrumental practice
Authors: López-Íñiguez Guadalupe , McPherson Gary E.
Published: April 2024

The strategies that enable musicians to adapt their behaviors so that they can break through, feel energized, and perform well collectively distinguish what it is to be a self-regulated learner. These strategies range from one’s ability to monitor thoughts and actions to being able to navigate and control one’s emotions, especially when feeling frustrated or anxious. Given the challenges of the music profession, it becomes imperative for teachers to equip their students with the necessary skills to self-regulate their own actions, feelings, and thinking so that they are eventually able to cope with the demands required of a contemporary professional musical career. In this study, we focused on the self-regulatory engagement of four master’s level cellists who were enrolled in a prominent European higher music education institution. Our data comprised self-regulated learning-based diary-reports that describes the students’ practice of self-chosen, especially demanding passages as they prepared for a public recital. Results depict differences between the musicians according to the efficiency of their practice leading up to a formal public recital.
Long-term care needs and hospitalization costs with long-term care insurance: a mixed-sectional study
Authors: Che Tiantian , Li Jia , Li Jun , Chen Xiaobo , Liao Zangyi
Published: April 2024

Background

With the rapid aging of the population, the health needs of the older adult have increased significantly, resulting in the frequent occurrence of the “social hospitalization” problem, which has led to a rapid increase in hospitalization costs. This study investigates whether the “social hospitalization problem” arising from the long-term care needs can be solved through the implementation of long-term care insurance, thereby improving the overall health of the older adults and controlling the unreasonable increase in hospitalization costs.

Methods

The entropy theory was used as a conceptual model, based on data from the China Health and Retirement Longitudinal Study (CHARLS) in 2015 and 2018. The least-squares method was used to examine the relationship between long-term care needs and hospitalization costs, and the role that long-term care insurance implementation plays in its path of influence.

Results

The results of this study indicated that long-term care needs would increase hospitalization cost, which remained stable after a series of tests, such as replacing the core explanatory variables and introducing fixed effects. Through the intermediary effect test and mediated adjustment effect test, we found the action path of long-term care needs on hospitalization costs. Long-term care needs increases hospitalization costs through more hospitalizations. Long-term care insurance reduces hospitalization costs. Its specific action path makes long-term care insurance reduce hospitalization costs through a negative adjustment of the number of hospitalizations.

Conclusion

To achieve fair and sustainable development of long-term care insurance, the following points should be achieved: First, long-term care insurance should consider the prevention in advance and expand the scope of participation and coverage; Second, long-term care insurance should consider the control in the event and set moderate levels of treatment payments; Third, long-term care insurance should consider post-supervision and explore appropriate payment methods.
Understanding dynamics and overlapping epidemiologies of HIV, HSV-2, chlamydia, gonorrhea, and syphilis in sexual networks of men who have sex with men
Authors: Omori Ryosuke , Chemaitelly Hiam , Abu-Raddad Laith J.
Published: April 2024

Introduction

We aimed to investigate the overlapping epidemiologies of human immunodeficiency virus (HIV), herpes simplex virus type 2 (HSV-2), chlamydia, gonorrhea, and syphilis in sexual networks of men who have sex with men (MSM), and to explore to what extent the epidemiology of one sexually transmitted infection (STI) relates to or differs from that of another STI.

Methods

An individual-based Monte Carlo simulation model was employed to simulate the concurrent transmission of STIs within diverse sexual networks of MSM. The model simulated sexual partnering, birth, death, and STI transmission within each specific sexual network. The model parameters were chosen based on the current knowledge and understanding of the natural history, transmission, and epidemiology of each considered STI. Associations were measured using the Spearman’s rank correlation coefficient (SRCC) and maximal information coefficient (MIC).

Results

A total of 500 sexual networks were simulated by varying the mean and variance of the number of partners for both short-term and all partnerships, degree correlation, and clustering coefficient. HSV-2 had the highest current infection prevalence across the simulations, followed by HIV, chlamydia, syphilis, and gonorrhea. Threshold and saturation effects emerged in the relationship between STIs across the simulated networks, and all STIs demonstrated moderate to strong associations. The strongest current infection prevalence association was between HIV and gonorrhea, with an SRCC of 0.84 (95% CI: 0.80–0.87) and an MIC of 0.81 (95% CI: 0.74–0.88). The weakest association was between HSV-2 and syphilis, with an SRCC of 0.54 (95% CI: 0.48–0.59) and an MIC of 0.57 (95% CI, 0.49–0.65). Gonorrhea exhibited the strongest associations with the other STIs while syphilis had the weakest associations. Across the simulated networks, proportions of the population with zero, one, two, three, four, and five concurrent STI infections were 48.6, 37.7, 11.1, 2.4, 0.3, and < 0.1%, respectively. For lifetime exposure to these infections, these proportions were 13.6, 21.0, 22.9, 24.3, 13.4, and 4.8%, respectively.

Conclusion

STI epidemiologies demonstrate substantial overlap and associations, alongside nuanced differences that shape a unique pattern for each STI. Gonorrhea exhibits an “intermediate STI epidemiology,” reflected by the highest average correlation coefficient with other STIs.
Public awareness and understanding of stem cell treatments available in Saudi Arabia and their trust in hospitals and research centers involved in stem cell research—a cross sectional study
Authors: Aboalola Doaa , Ramadan Majed , Baadhaim Moayad , Alsiary Rawiah , Badraiq Heba , Alghamdi Tariq , Zakri Samer , Aboulola Neda , Falatah Tark , Malibari Dalal
Published: April 2024

Introduction

Although stem cell research and therapeutic applications hold great promise for medical advancements, and have rapidly progressed globally, there remains a lack of genuine public awareness of the status of this subject in Saudi Arabia. Successful integration of stem cell therapy into healthcare relies on public awareness, understanding, and trust. Therefore, we aimed in this cross-sectional study to assess the public’s knowledge, awareness, trust, support, participation, and confidence in stem cell treatments and centers involved in it.

Materials and methods

A voluntary questionnaire of 20 questions was distributed randomly via social media outlets.

Results

Three thousand five hundred eighty four individuals participated in the survey, with approximately half of them falling within the age range of 35–50 years (46.71%). Majority of the participants, 90.71%, would like to know more about stem cell therapy and more than half of the participants (56.94%) were unfamiliar with the idea, and a comparable proportion (50.41%) expressed concerns about the safety of stem cell therapy. A lower level of awareness, indicated by a score of 5, was evenly distributed across all age groups and genders. However, regardless of gender, older participants—especially those 50 years of age or older—tended to report higher levels of confidence, trust, and support than participants in other age groups. Moreover, trust, support, participation, and confidence score for those attained high school or less was statistically significantly lower than those attained master’s or PhD degree. Of the participants, 33.57% had either received stem cell therapy themselves or known someone who had; about 24.07% of them reported that it was a cosmetic type of treatment.

Conclusion

The study emphasizes the persistent need for awareness and educational initiatives to minimize the lack of public awareness and understanding of approved stem cell treatments in Saudi Arabia. It advocates for increased education, transparency, and communication to bridge knowledge gaps and enhance public trust to ensure the understanding of successful treatment.
Analysis of patient safety event report categories at one large academic hospital
Authors: Mitchell Cody , Butler Logan , Holloway Alexa D. , Ra Jin H. , Adapa Karthik , Greenberg Caprice , Marks Lawrence B. , Ivester Thomas , Mazur Lukasz
Published: April 2024

Given the persistent safety incidents in operating rooms (ORs) nationwide (approx. 4,000 preventable harmful surgical errors per year), there is a need to better analyze and understand reported patient safety events. This study describes the results of applying the Team Strategies and Tools to Enhance Performance and Patient Safety (TeamSTEPPS) supported by the Teamwork Evaluation of Non-Technical Skills (TENTS) instrument to analyze patient safety event reports at one large academic medical center. Results suggest that suboptimal behaviors stemming from poor communication, lack of situation monitoring, and inappropriate task prioritization and execution were implicated in most reported events. Our proposed methodology offers an effective way of programmatically sorting and prioritizing patient safety improvement efforts.
Judo for older adults: the coaches' knowledge and needs of education
Authors: Ciaccioni Simone , Guidotti Flavia , Palumbo Federico , Forte Roberta , Galea Envic , Sacripanti Attilio , Lampe Nuša , Lampe Špela , Jelušić Toma , Bradić Slaviŝa , Lascau Maria-Loredana , Rodica-Borza Alina , Camacho Pérez Raúl , Diéguez Rodríguez-Montero Fernando , Kapan Mesut , Gezeker Kaya , Capranica Laura , Tessitore Antonio
Published: April 2024

This study aimed to explore the views of judo coaches on their perceived knowledge (PK) and needs for education (NE) for training older practitioners. In total, 470 international (Europe = 48%, Americas = 22%, Africa = 23%, Asia = 5% and Oceania = 2%) judo coaches (IJF: level 1 = 55,3%, level 2 = 33%; judo black belt: 3,4 ± 1,7 dan; F = 15%; university education: 68% >BA) responded an online survey encompassing demographic information and 35 items relevant to training older adults (Aging process; Safety and First Aid; Organization & Environment; Physiology and Fitness; Psychology & Mental Health; Teaching & Training) to be rated on a 7-point Likert scale for PK and NE. Non parametric statistics (p > 0.05) was applied to ascertain differences and relationships between PK and NE, respectively. A bivariate go-zone plot was used to highlight items with the lowest PK and the highest NE mean values. The coaches reported high PK (4.5 ± 0.3 pt) and NE (4.7 ± 0.1 pt) values, with significant higher PK values emerging for high education levels and judo experience. In considering their unique needs and special role, the judo coaches presented valuable insights to develop a sustainable educational curriculum tailored to train older judo practitioners.
Exploring altered bovine sperm trajectories by sperm tracking in unconfined conditions
Authors: Canonico Luigi Fausto , De Clemente Claudia , Fardilha Margarida , Ferreira Ana Filipa , Maremonti Maria Isabella , Dannhauser David , Causa Filippo , Netti Paolo Antonio
Published: April 2024

Mammalian sperm motility is getting more relevant due to rising infertility rates worldwide, generating the need to improve conventional analysis and diagnostic approaches. Nowadays, computer assisted sperm analysis (CASA) technologies represent a popular alternative to manual examination which is generally performed by observing sperm motility in very confined geometries. However, under physiological conditions, sperm describe three-dimensional motility patterns which are not well reconstructed by the limited depth of standard acquisition chambers. Therefore, affordable and more versatile alternatives are needed. Here, a motility analysis in unconfined conditions is proposed. In details, the analysis is characterized by a significant longer duration -with respect to conventional systems- with the aim to observe eventually altered motility patterns. Brightfield acquisition in rectangular glass capillaries captured frozen–thawed bovine spermatozoa which were analyzed by means of a self-written tracking routine and classified in sub-populations, based on their curvilinear velocity. To test the versatility of our approach, cypermethrin -a commonly used pesticides- known to be responsible for changes in sperm motility was employed, assessing its effect at three different time-steps. Experimental results showed that such drug induces an increase in sperm velocity and progressiveness as well as circular pattern formation, likely independent of wall interactions. Moreover, this resulted in a redistribution of sperm with the rapid class declining in number with time, but still showing an overall velocity increase. The flexibility of the approach permits parameter modifications with the experimental needs, allowing us to conduct a comprehensive examination of sperm motility. This adaptability facilitated data acquisition which can be computed at different frame rates, extended time periods, and within deeper observation chambers. The suggested approach for sperm analysis exhibits potential as a valuable augmentation to current diagnostic instruments.
Evolutionary engineering and characterization of Sendai virus mutants capable of persistent infection and autonomous production
Authors: Iwata Moe , Kawabata Ryoko , Morimoto Nao , Takeuchi Ryosuke F. , Sakaguchi Takemasa , Irie Takashi , Osakada Fumitaka
Published: April 2024

Persistent virus infection involves modifying the host immune response and maintaining viral infection. Acute infection with Mononegavirales, such as Sendai viruses (SeVs), can give rise to viruses capable of persistent infection. SeVs establish persistent infection through generating copyback-type defective interfering (cbDI) genomes or acquiring temperature-sensitive mutations. Herein, we identify novel mutations associated with persistent infection and recombinant SeV mutants capable of persistent infection and autonomous production at physiological body temperature, independent of cbDI genomes or temperature-sensitive mutations. Diverse SeV populations were generated by passing the cDNA-recovered SeV Z strain 19 times through embryonated chicken eggs and subsequently infecting LLC-MK2 cells with the SeV populations to finally obtain SeV mutants capable of persistent infection and autonomous production in several types of cultured cells. Sequence analysis identified 4 or 5 mutations in the genome of the persistently infectious SeVs, distinguishing them from other existing strains with persistent infection. Recombinant SeVs carrying 4 or 5 mutations in the Z strain genome (designated SeV-Zpi or SeV-Zpi2, respectively) exhibited persistent infection and autonomous production in LLC-MK2, BHK-21, and Neuro2a cells at 37°C. SeV-Zpi and SeV-Zpi2 consistently produced viral particles even after long-term passages without cbDI particles or temperature-sensitive phenotypes. These results highlight the ability of acute infections of SeVs to spontaneously acquire mutations during replication, thereby endowing persistent infection and autonomous production at body temperature. The vectorization of SeV-Zpi and SeV-Zpi2 will contribute to both basic research and medical applications.
Research on Path Tracking of Unmanned Spray Based on Dual Control Strategy
Authors: Wen Haojun , Ma Xiaodong , Qin Chenjian , Chen Hao , Kang Huanyu
Published: April 2024

The high clearance spray is a type of large and efficient agricultural machinery used for plant protection, and path tracking control is the key to ensure the efficient and safe operation of spray. Sliding mode control and other methods are commonly used abroad to track vehicles, while fuzzy control, neural networks and other methods are commonly used at home. However, domestic and foreign research on autonomous agricultural machinery is mainly focused on tractors and other machinery, while research on self-propelled spray in high clearance is less abundant. This paper takes the path tracking algorithm in the integrated navigation system of spray as the main research goal, studies the path tracking control algorithm for straight lines and turning curves that can realize the automatic driving of spray by establishing the path tracking algorithm for unmanned spray based on dual control strategies, designs the path tracking controller, including the preview model theoretical path tracking controller and variable domain fuzzy controller, and determines the preview model through the design of the preview model theoretical path tracking controller. The lateral and longitudinal errors of the model algorithm are analyzed, and the driving characteristics under the complex spray road surface are analyzed. The design of the variable domain fuzzy predictor theory path tracking controller is proposed, and the design of the road model selection controller is calculated and analyzed in detail, including the determination of the road roughness coefficient and the selection of the range of the difference between the average value of the excitation before and after sampling, which improves the performance of the spray path tracking algorithm. The experiment shows that the proposed path tracking control algorithm can meet the path tracking requirements of unmanned spray in the current road environment, and provide a reliable solution for the automatic control of high clearance spray.
Diagnosis and Management of Congenital H-Type Tracheoesophageal Fistula: Results of a National Survey
Authors: Morchio Cecilia , Ganarin Alba , Conforti Andrea , Leva Ernesto , Gaglione Giovanni , Brenco Gaia , Zambaiti Elisa , Chiarenza Salvatore Fabio , Caldaro Tamara , Cheli Maurizio , Boroni Giovanni , Marcandella Elena Sofia , Riccipetitoni Giovanna , Cacciaguerra Sebastiano , Di Benedetto Vincenzo , Gentilino Valerio , Lisi Gabriele , Morini Francesco , Midrio Paola
Published: April 2024

Background: Congenital h-type tracheoesophageal fistula (H-TEF) without esophageal atresia (EA) represents about 4% of congenital esophageal anomalies. The diagnosis is challenging, and surgery is considered curative. The aim was to report a national survey on the diagnosis, management, and outcome of patients with congenital H-TEF. Methods: Following approval of the Italian Society of Pediatric Surgery, a survey was sent to all Pediatric Surgery Units to retrospectively collect H-TEF treated in the period 2010–2022. Descriptive analysis was performed, and results are given as prevalence, mean ± standard deviation (SD), or median and interquartile range (IQR). Results: The survey was sent to 65 units. Seventeen responded with one or more cases; 78 patients were diagnosed with H-TEF during the study period. Associated malformations were present in 43%, mostly cardiac (31%). The most frequent symptoms were cough (36%), bronchopneumonia (24%), and dysphagia (19%). H-TEF was detected by tracheobronchoscopy (90%), and/or upper GI (58%), and/or esophagoscopy (32%). The median age at diagnosis was 23 days (1 day–18 years). The most common approach was cervicotomy (76%), followed by thoracoscopy (14%) and thoracotomy (9%). The fistula underwent ligation and section of the fistula in 90% of the patients and clip closure and section in 9%. In one patient, the fistula was cauterized endoscopically. H-TEF preoperative cannulation was performed in 68% of cases, and a drain was placed in 26%. One month after surgery, 13% of the patients had mild persisting symptoms, mainly hypophonia. Recurrence occurred in 5%, and a second recurrence occurred in 1%. Conclusions: H-TEF prevalence was six cases/year, consistent with the expected rate of five cases/year in our country. The diagnosis was challenging, sometimes delayed, and, in most patients, required multiple examinations. Fistula ligation and section through cervicotomy were the most frequent treatment. Long-term outcomes are good, and recurrence is a rare event.
Patient Characteristics, Treatment, and Survival in Pleuropulmonary Blastoma: An Analysis from the National Cancer Database
Authors: Chidiac Charbel , Sferra Shelby R. , Kunisaki Shaun M. , Rhee Daniel S.
Published: April 2024

Pleuropulmonary blastoma (PPB) is a rare childhood tumor originating from the lung or pleura, typically treated with surgery, chemotherapy (CTx), and/or radiation therapy (RTx). This study aimed to assess patient and tumor features, treatment methods, and survival rates in PPB. We retrospectively analyzed PPB patients under 18 from 2004 to 2019, using the National Cancer Database (NCDB). We assessed 5-year overall survival (OS) rates based on disease presentation and treatment regimens, using Kaplan–Meier curves and Cox proportional regression. Among 144 cases identified, 45.9% were female, with a median age of 2 years (interquartile range 1–3). In all, 62.5% of tumors originated from the lung, and 10.4% from the pleura. Moreover, 6.9% were bilateral, and the median tumor size was 8.9 cm, with 4.2% presenting with metastases. The 5-year OS rate was 79.6%, with no significant change over time (2004–2009, 77.6%; 2010–2014, 90.8%; 2015–2019, OS 75.3%; p = 0.08). In all, 5.6% received CTx alone, 31.3% surgery alone, 45.8% surgery/CTx, and 17.4% surgery/CTx/RTx. The OS rates were comparable between the surgery/CTx/RTx (80.0%) and surgery/CTx (76.5%) groups (adjusted Hazard Ratio, 1.72; 95% CI, 0.57–5.19; p = 0.34). Therefore, due to the unchanged survival rates over time, further prospective multicenter studies are needed to determine the optimal multimodal treatment regimens for this rare pediatric tumor.
Improvement of Methods and Devices for Multi-Parameter High-Voltage Testing of Dielectric Coatings
Authors: Syasko Vladimir , Musikhin Alexey , Gnivush Igor
Published: April 2024

Currently, the high voltage testing method is widely used to detect pinholes and porosity defects in dielectric coatings. However, most modern coatings also have requirements for the minimum allowable coating thickness. Conducting tolerance tests on the thickness of dielectric coatings concurrently along with monitoring integrity within a single technological process appears promising. Additionally, mitigating the impact of various interfering parameters is crucial. This paper conducts a theoretical and experimental examination of spark formation processes in both gas and dielectrics. This analysis takes place during the identification of both through and non-through defects in dielectric coatings on conductive substrates. The principles of selecting the test voltage for the investigated dielectric coatings, considering the need to detect both through defects and inadmissible thinning, are theoretically and experimentally justified. It is suggested to utilize a probabilistic approach for evaluating the detectability of the mentioned defects. It is demonstrated that, when the dielectric strength of the coating is known, it is feasible to identify both through and non-through defects in coatings with a calculated probability under a specified test voltage. The conditions of occurrence of partial discharges in the process of testing are investigated, and measures to suppress their influence on the inspection results are proposed. The influence of the substrate surface roughness on the magnitude of the breakdown voltage during testing is considered.
Superconductors without Symmetry Breaking
Authors: Diamantini Maria Cristina
Published: April 2024

We review the main features of type-III superconductivity. This is a new type of superconductivity that exists in both 2 and 3 spatial dimensions. The main characteristics are emergent granularity and the superconducting gap being opened by a topological mechanism, with no Higgs field involved. Superconductivity is destroyed by the proliferation of vortices and not by the breaking of Cooper pairs, which survive above the critical temperature. The hallmark of this superconductivity mechanism, in 3 spatial dimensions (3D), is the Vogel–Fulcher–Taman scaling of the resistance with temperature.
A Sustainable Multistage Process for Immobilizing Bioactive Compounds on Layered Double Hydroxides
Authors: Coiai Serena , Passaglia Elisa , Telleschi Alice , Oberhauser Werner , Coltelli Maria-Beatrice , Cicogna Francesca
Published: April 2024

Hybrid systems with antioxidant properties have been developed by integrating bioactive compounds derived from plant resources with layered double hydroxides (LDHs). Anion exchange has been used to substitute intercalated nitrate anions in Mg-Al LDH with carboxylate anions derived from trans-ferulic acid, rosmarinic acid, and 18β-glycyrrhetinic acid. These organic compounds are known for their powerful antioxidant, anti-inflammatory and antimicrobial properties and are highly suitable for cosmetics, biomedicine, and food packaging. To enhance sustainability, a multistage procedure has been developed with the aim of recovering unexchanged carboxylate anions from residual reaction water, ensuring an environmentally friendly and easily scalable preparation process. The process, adapted for each of the three molecules, allows the production of a consistently high-quality hybrid product containing an organic fraction ranging from 10 to 48% by weight, depending on the specific molecule used. The immobilization of organic compounds has occurred either within the layers of LDH through intercalation or on the external surface through adsorption. Good antioxidant capacity has been exhibited by these powdered hybrid systems, as assessed through both the DPPH and linoleic acid/β-carotene tests. Sustainable production practices are enabled by this innovative approach, which also opens avenues for the development of advanced materials for diverse applications across various industries.
Dryland Soil Carbon and Nitrogen Stocks in Response to Cropping Systems and Nitrogen Fertilization
Authors: Sainju Upendra M.
Published: April 2024

Innovative management practices are needed to mitigate greenhouse gas (GHG) emissions from the agricultural sector by enhancing soil carbon (C) and nitrogen (N) stocks, which serve as major reservoirs of C and N in the terrestrial ecosystem. The effect of cropping systems and N fertilization rates were examined on soil organic C (SOC) and soil total N (STN) stocks at the 0–120 cm depth from 2011 to 2018 in a dryland farm in the US northern Great Plains. Cropping systems were no-till continuous spring wheat (Triticum aestivum L.) (NTCW), no-till spring wheat–pea (Pisum sativum L.) (NTWP), no-till spring wheat–fallow (NTWF), and conventional till spring wheat–fallow (CTWF) and N fertilization rates were 0, 50, 100, and 150 kg N ha−1 applied to spring wheat. The SOC and STN were greater for NTWP than other cropping systems at most N fertilization rates and depth layers. Increasing N fertilization rate increased SOC at 0–30 cm for NTWP and NTCW, but had a variable effect on STN for various cropping systems and soil depths. The NTWP with 50–100 kg N ha−1 can enhance SOC and STN at 0–30 cm compared to other cropping systems and N fertilization rates in the US northern Great Plains.
Alginate- and Chitosan-Modified Gelatin Hydrogel Microbeads for Delivery of E. coli Phages
Authors: Moghtader Farzaneh , Solakoglu Sencer , Piskin Erhan
Published: April 2024

Bacterial infections are among the most significant health problems/concerns worldwide. A very critical concern is the rapidly increasing number of antibiotic-resistant bacteria, which requires much more effective countermeasures. As nature’s antibacterial entities, bacteriophages shortly (“phages”) are very important alternatives to antibiotics, having many superior features compared with antibiotics. The development of phage-carrying controlled-release formulations is still challenging due to the need to protect their activities in preparation, storage, and use, as well as the need to create more user-friendly forms by considering their application area/site/conditions. Here, we prepared gelatin hydrogel microbeads by a two-step process. Sodium alginate was included for modification within the initial recipes, and these composite microbeads were further coated with chitosan. Their swelling ratio, average diameters, and Zeta potentials were determined, and degradations in HCl were demonstrated. The target bacteria Escherichia coli (E.coli) and its specific phage (T4) were obtained from bacterial culture collections and propagated. Phages were loaded within the microbeads with a simple method. The phage release characteristics were investigated comparatively and were demonstrated here. High release rates were observed from the gelatin microbeads. It was possible to reduce the phage release rate using sodium alginate in the recipe and chitosan coating. Using these gelatin-based microbeads as phage carrier matrices—especially in lyophilized forms—significantly improved the phage stability even at room temperature. It was concluded that phage release from gelatin hydrogel microbeads could be further controlled by alginate and chitosan modifications and that user-friendly lyophilized phage formulations with a much longer shelf life could be produced.