bluuebunny/crossref_metadata_2025 huggingface dataset:
Streptococcus mitis and Prevotella melaninogenica Influence Gene Expression Changes in Oral Mucosal Lesions in Periodontitis Patients
Authors: Tomic Uros , Nikolic Nadja , Carkic Jelena , Mihailovic Djordje , Jelovac Drago , Milasin Jelena , Pucar Ana
Published: September 2023

Oral microbiome disruptions in periodontitis are related to the chronic inflammatory reactions that could in turn lead to the development of multiple oral diseases. The objective of the study was to assess the frequencies of Streptococcus mitis, Prevotella melaninogenica, and Prevotella intermedia in oral benign lesions, oral potentially malignant disorders (OPMDs), and oral squamous cell carcinomas (OSCCs) and investigate the impact of these bacteria on the expression patterns of the selected (potential) target genes (PI3CA/AKT2/mTOR, DUSP16/MAPK14, and COX2). After sample collection (25 benign lesions, 30 OPMDs, and 35 OSCCs) and DNA/RNA extraction, quantitative real-time polymerase chain reaction (qPCR) was performed to detect bacterial presence and assess relative gene expression levels in different lesion groups. Prevotella melaninogenica was the most prevalent of the three analyzed bacteria, with the frequency being 60% in benign lesions, 87% in OPMDs (p = 0.024), and 77% in OSCC. The OPMD tissues in which Prevotella melaninogenica was present exhibited a higher expression level of AKT2 (p = 0.042). Significantly lower expression of DUSP16 was observed in OSCC tissues containing Streptococcus mitis (p = 0.011). The obtained results indicate a substantial contribution of P. melaninogenica and Str. mitis in the pathogenesis of oral mucosal lesions, possibly via AKT2 upregulation and DUSP16 downregulation.
Nanocarriers in Tuberculosis Treatment: Challenges and Delivery Strategies
Authors: Kumar Mahesh , Virmani Tarun , Kumar Girish , Deshmukh Rohitas , Sharma Ashwani , Duarte Sofia , Brandão Pedro , Fonte Pedro
Published: September 2023

The World Health Organization identifies tuberculosis (TB), caused by Mycobacterium tuberculosis, as a leading infectious killer. Although conventional treatments for TB exist, they come with challenges such as a heavy pill regimen, prolonged treatment duration, and a strict schedule, leading to multidrug-resistant (MDR) and extensively drug-resistant (XDR) strains. The rise of MDR strains endangers future TB control. Despite these concerns, the hunt for an efficient treatment continues. One breakthrough has been the use of nanotechnology in medicines, presenting a novel approach for TB treatment. Nanocarriers, such as lipid nanoparticles, nanosuspensions, liposomes, and polymeric micelles, facilitate targeted delivery of anti-TB drugs. The benefits of nanocarriers include reduced drug doses, fewer side effects, improved drug solubility, better bioavailability, and improved patient compliance, speeding up recovery. Additionally, nanocarriers can be made even more targeted by linking them with ligands such as mannose or hyaluronic acid. This review explores these innovative TB treatments, including studies on nanocarriers containing anti-TB drugs and related patents.
Preclinical Evaluation of an Imidazole-Linked Heterocycle for Alzheimer’s Disease
Authors: Bagán Andrea , Rodriguez-Arévalo Sergio , Taboada-Jara Teresa , Griñán-Ferré Christian , Pallàs Mercè , Brocos-Mosquera Iria , Callado Luis F. , Morales-García José A. , Pérez Belén , Diaz Caridad , Fernández-Godino Rosario , Genilloud Olga , Beljkas Milan , Oljacic Slavica , Nikolic Katarina , Escolano Carmen
Published: September 2023

Humanity is facing a vast prevalence of neurodegenerative diseases, with Alzheimer’s disease (AD) being the most dominant, without efficacious drugs, and with only a few therapeutic targets identified. In this scenario, we aim to find molecular entities that modulate imidazoline I2 receptors (I2-IRs) that have been pointed out as relevant targets in AD. In this work, we explored structural modifications of well-established I2-IR ligands, giving access to derivatives with an imidazole-linked heterocycle as a common key feature. We report the synthesis, the affinity in human I2-IRs, the brain penetration capabilities, the in silico ADMET studies, and the three-dimensional quantitative structure-activity relationship (3D-QSAR) studies of this new bunch of I2-IR ligands. Selected compounds showed neuroprotective properties and beneficial effects in an in vitro model of Parkinson’s disease, rescued the human dopaminergic cell line SH-SY5Y from death after treatment with 6-hydroxydopamine, and showed crucial anti-inflammatory effects in a cellular model of neuroinflammation. After a preliminary pharmacokinetic study, we explored the action of our representative 2-(benzo[b]thiophen-2-yl)-1H-imidazole LSL33 in a mouse model of AD (5xFAD). Oral administration of LSL33 at 2 mg/Kg for 4 weeks ameliorated 5XFAD cognitive impairment and synaptic plasticity, as well as reduced neuroinflammation markers. In summary, this new I2-IR ligand that promoted beneficial effects in a well-established AD mouse model should be considered a promising therapeutic strategy for neurodegeneration.
Conceptual Framework of the Design of Pleiotropic Drugs against Alzheimer’s Disease
Authors: Guiselin Thomas , Lecoutey Cédric , Rochais Christophe , Dallemagne Patrick
Published: September 2023

The multifactorial nature of some diseases, particularly neurodegenerative diseases such as Alzheimer’s disease, frequently requires the use of several drugs. These drug cocktails are not without drawbacks in terms of increased adverse effects, drug–drug interactions or low adherence to treatment. The use of pleiotropic drugs, which combine, within a single molecule, several activities directed against distinct therapeutic targets, makes it possible to overcome some of these problems. In addition, these pleiotropic drugs generally lead to the expression of a synergy of effects, sometimes greater than that observed with a combination of drugs. This article will review, through recent examples, the different kinds of pleiotropic drugs being studied or already present on the market of medicines, with a focus on the structural aspect of such drug design.
The Editorial Position on ‘Recent Advances in Multifunctional Antimicrobial Peptides as Preclinical Therapeutic Studies and Clinical Future Applications’
Authors: Scavello Francesco , Amiche Mohamed , Ghia Jean-Eric
Published: September 2023

Antibiotic resistance has recently been recognized as an alarming issue and one of the leading causes of death worldwide [...]
A High-Security Probabilistic Constellation Shaping Transmission Scheme Based on Recurrent Neural Networks
Authors: Zhou Shuyu , Liu Bo , Ren Jianxin , Mao Yaya , Wu Xiangyu , Guo Zeqian , Zhu Xu , Ding Zhongwen , Wu Mengjie , Wang Feng , Ullah Rahat , Wu Yongfeng , Zhao Lilong , Li Ying
Published: September 2023

In this paper, a high-security probabilistic constellation shaping transmission scheme based on recurrent neural networks (RNNs) is proposed, in which the constellation point probabilistic distribution is generated based on recurrent neural network training. A 4D biplane fractional-order chaotic system is introduced to ensure the security performance of the system. The performance of the proposed scheme is verified in a 2 km seven-core optical transmission system. The RNN-trained probabilistic shaping scheme achieves a transmission gain of 1.23 dB compared to the standard 16QAM signal, 0.39 dB compared to the standard Maxwell-Boltzmann (M-B) distribution signal, and a higher net bit rate. The proposed encryption scheme has higher randomness and security than the conventional integer-order chaotic system, with a key space of 10,163. This scheme will have a promising future fiber optic transmission scheme because it combines the efficient transmission and security of fiber optic transmission systems.
Phytochemical Composition and Pharmacological Activities of Three Essential Oils Collected from Eastern Morocco (Origanum compactum, Salvia officinalis, and Syzygium aromaticum): A Comparative Study
Authors: Loukili El Hassania , Ouahabi Safae , Elbouzidi Amine , Taibi Mohamed , Yahyaoui Meryem Idrissi , Asehraou Abdeslam , Azougay Abdellah , Saleh Asmaa , Al Kamaly Omkulthom , Parvez Mohammad Khalid , El Guerrouj Bouchra , Touzani Rachid , Ramdani Mohammed
Published: September 2023

Throughout history, essential oils have been employed for their pleasing scents and potential therapeutic benefits. These oils have shown promise in various areas, including aromatherapy, personal care products, natural remedies, and even as alternatives to traditional cleaning agents or pest control solutions. The study aimed to explore the chemical makeup, antioxidant, and antibacterial properties of Origanum compactum Benth., Salvia officinalis L., and Syzygium aromaticum (L.) Merr. et Perry. Initially, the composition of the three essential oils, O. compactum (HO), S. officinalis (HS), and S. aromaticum (HC) was analyzed using GC-MS technology, revealing significant differences in the identified compounds. α-thujone emerged as the predominant volatile component in the oils, making up 78.04% of the composition, followed by eugenol, which constituted 72.66% and 11.22% of the HC and HO oils, respectively. To gauge antioxidant capabilities, tests involving DPPH scavenging capacity and total antioxidant capacity were conducted. Antioxidant activity was determined through the phosphomolybdate test and the DPPH• radical scavenging activity, with the HO essential oil displaying significant scavenging capacity (IC50 of 0.12 ± 0.02 mg/mL), similar to ascorbic acid (IC50 of 0.26 ± 0.24 mg/mL). Similarly, the TAC assay for HO oil revealed an IC50 of 1086.81 ± 0.32 µM AAE/mg. Additionally, the oils’ effectiveness against four bacterial strains, namely Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Listeria monocytogenes, and five fungi, Geotrichum candidum, Aspergillus niger, Saccharomyces cerevisiae, Candida glabrata, and Candida albicans, was tested in vitro. The examined essential oils generally exhibited limited antimicrobial effects, with the exception of HC oil, which demonstrated an exceptionally impressive level of antifungal activity. In order to clarify the antioxidant, antibacterial, and antifungal effects of the identified plant compounds, we employed computational methods, specifically molecular docking. This technique involved studying the interactions between these compounds and established protein targets associated with antioxidant, antibacterial, and antifungal activities.
Effects of Individual or Combined Deficiency of Phosphorous and Zinc on Phenotypic, Nutrient Uptake, and Molecular Responses of Finger Millet (Eleusine coracana): A Nutri-Rich Cereal Crop
Authors: Maharajan Theivanayagam , Krishna Thumadath Palayullaparambil Ajeesh , Shilpha Jayabalan , Ceasar Stanislaus Antony
Published: September 2023

Deficiencies of either phosphorus (P) or zinc (Zn) or both are one of the major abiotic constraints influencing agricultural production. Research on the effects of individual or combined P and Zn deficiency is limited in cereals. This study reports the effects of the individual or combined deficiency of inorganic phosphate (Pi) and Zn on the phenotypic, root hair modification, nutrient uptake, and molecular responses of finger millet (Eleusine coracana), a nutri-rich cereal crop. Finger millet seedlings were grown hydroponically under control (+Pi+Zn), individual Pi deficiency (−Pi), individual Zn deficiency (−Zn), and combined Pi and Zn deficiency (−Pi−Zn) conditions for 30 days to find the phenotypic, root hair modification, nutrient uptake, and molecular responses. Compared to the individual −Zn condition, the individual −Pi condition had more of an effect in terms of biomass reduction. The combined −Pi−Zn condition increased the root hair length and density compared to the other three conditions. The individual −Zn condition increased the Pi uptake, while the individual −Pi condition favored the Zn uptake. EcZIP2 was highly upregulated in shoot tissues under the individual −Zn condition, and EcPHT1;2 was highly expressed in root tissues under the individual −Pi condition. This is the first study to report the effects of the individual or combined deficiency of Pi and Zn in finger millet and may lead to future studies to better manage P and Zn deficiency.
Plant Stomata: An Unrealized Possibility in Plant Defense against Invading Pathogens and Stress Tolerance
Authors: Meddya Sandipan , Meshram Shweta , Sarkar Deepranjan , S Rakesh , Datta Rahul , Singh Sachidanand , Avinash Gosangi , Kumar Kondeti Arun , Savani Ajit Kumar , Thulasinathan Thiyagarajan
Published: September 2023

Stomata are crucial structures in plants that play a primary role in the infection process during a pathogen’s attack, as they act as points of access for invading pathogens to enter host tissues. Recent evidence has revealed that stomata are integral to the plant defense system and can actively impede invading pathogens by triggering plant defense responses. Stomata interact with diverse pathogen virulence factors, granting them the capacity to influence plant susceptibility and resistance. Moreover, recent studies focusing on the environmental and microbial regulation of stomatal closure and opening have shed light on the epidemiology of bacterial diseases in plants. Bacteria and fungi can induce stomatal closure using pathogen-associated molecular patterns (PAMPs), effectively preventing entry through these openings and positioning stomata as a critical component of the plant’s innate immune system; however, despite this defense mechanism, some microorganisms have evolved strategies to overcome stomatal protection. Interestingly, recent research supports the hypothesis that stomatal closure caused by PAMPs may function as a more robust barrier against pathogen infection than previously believed. On the other hand, plant stomatal closure is also regulated by factors such as abscisic acid and Ca2+-permeable channels, which will also be discussed in this review. Therefore, this review aims to discuss various roles of stomata during biotic and abiotic stress, such as insects and water stress, and with specific context to pathogens and their strategies for evading stomatal defense, subverting plant resistance, and overcoming challenges faced by infectious propagules. These pathogens must navigate specific plant tissues and counteract various constitutive and inducible resistance mechanisms, making the role of stomata in plant defense an essential area of study.
Allelopathic Activity of a Novel Compound, Two Known Sesquiterpenes, and a C13 Nor-Isopenoid from the Leave of Croton oblongifolius Roxb. for Weed Control
Authors: Moh Seinn Moh , Tojo Shunya , Teruya Toshiaki , Kato-Noguchi Hisashi
Published: September 2023

Investigation of allelopathic substances from herbal plants may lead to the development of allelochemical-based natural herbicides. Croton oblongifolius (Roxb.) is a well-known herbal plant with a long history of being used for traditional medicines and for being the source of a diverse range of bioactive compounds. This plant has been reported to have allelopathic potential; however, its allelopathic-related substances have not yet been described. Therefore, we conducted this investigation to explore the allelopathic substances from the leaves of C. oblongifolius. Aqueous methanol extracts of C. oblongifolius leaves exhibited significant growth inhibitory potential against four test plants (monocot barnyard grass and timothy, and dicot cress and lettuce). The leaf extracts were purified in various chromatographic steps and yielded four active compounds identified as (3R,6R,7E)-3-hydroxy-4-7-megastigmadien-9-one (I), 2-hydroxy alpinolide (a novel compound) (II), alpinolide (III), and epialpinolide (IV) via an analysis of the spectral data. These identified compounds significantly restricted the seedling growth of cress. The concentration necessary for 50% growth reduction of the cress seedlings varied from 0.15 to 0.24 mM for (3R,6R,7E)-3-hydroxy-4-7-megastigmadien-9-one, 0.04 to 0.11 mM for 2-hydroxy alpinolide, 0.07 to 0.12 mM for alpinolide, and 0.09 to 0.16 mM for epialpinolide. Therefore, the leaf extracts of C. oblongifolius and the characterized compounds have the potential to be used as weed-suppressive resources for natural weed control.
Phytochemical Analysis of Pinus cembra Heartwood—UHPLC-DAD-ESI-MSn with Focus on Flavonoids, Stilbenes, Bibenzyls and Improved HPLC Separation
Authors: Alperth Fabian , Schneebauer Anna , Kunert Olaf , Bucar Franz
Published: September 2023

The heartwood of the Swiss Stone Pine, Pinus cembra L., has been scarcely investigated for secondary metabolites for a long period of time. Considering age and relative simplicity of heartwood investigations dating back to the 1940s to 1960s, we conducted the first investigation of P. cembra heartwood by HPLC, using UHPLC-DAD-ESI-MSn and HPLC-DAD techniques in combination with isolation and NMR spectroscopy, with focus on stilbenes, bibenzyls and flavonoids. Analytical problems in the HPLC analysis of Pinus stilbenes and flavonoids on reversed stationary phases were also challenged, by comparing HPLC on pentafluorophenyl (PFP) and C18 stationary phases. Seven flavonoids (1, 2, 3, 7, 8, 11, 12), four stilbenes (4, 6, 10, 13), two bibenzyls (5, 9), three fatty acids (14, 16, 17) and one diterpenic acid (15) were detected in an ethanolic extract of Pinus cembra heartwood. HPLC comparison of reversed stationary phases in HPLC showed that the antifungal, antibacterial and chemosensitizing dihydropinosylvin monomethyl ether (9) and pinosylvin monomethyl ether (10) can be separated on PFP, but not on C18 material, when eluting with a screening gradient of 20–100% acetonitrile. Flavonoid separation showed additional benefits of combining analyses on different stationary phases, as flavonoids 7 and 8 could only be separated on one of two C18 stationary phases. Earlier phytochemical results for heartwood investigations were shown to be mostly correct, yet expandable. Substances 5 to 12 were found in alignment with these references, proving remarkable phytochemical analyses at the time. Evidence for the described presence of pinobanksin could not be found. Substances 1 to 4 and 13 have to our knowledge not yet been described for P. cembra.
Genome-Wide Analysis of the bHLH Gene Family in Loropetalum chinense var. rubrum: Identification, Classification, Evolution, and Diversity of Expression Patterns under Cultivation
Authors: Liu Yang , Lin Ling , Liu Yang , Mo Qiong , Zhang Damao , Li Weidong , Xiong Xingyao , Yu Xiaoying , Li Yanlin
Published: September 2023

The basic helix–loop–helix (bHLH) transcription factor family is the second-largest transcription factor family in plants. Members of this family are involved in the processes of growth and development, secondary metabolic biosynthesis, signal transduction, and plant resistance. Loropetalum chinense var. rubrum is a critical woody plant with higher ornamental and economic values, which has been used as ornamental architecture and traditional Chinese herbal medicine plants. However, the bHLH transcription factors in Loropetalum chinense var. rubrum (L. chinense var. rubrum) have not yet been systematically demonstrated, and their role in the biosynthesis of anthocyanin is still unclear. Here, we identified 165 potential LcbHLHs genes by using two methods, and they were unequally distributed on chromosomes 1 to 12 of the genome of L. chinense var. rubrum. Based on an evolutionary comparison with proteins from Arabidopsis and Oryza sativa, these bHLH proteins were categorized into 21 subfamilies. Most LcbHLHs in a particular subfamily had similar gene structures and conserved motifs. The Gene Ontology annotation and Cis-elements predicted that LcbHLHs had many molecular functions and were involved in processes of plant growth, including the biosynthesis of flavonoids and anthocyanins. Transcriptomic analysis revealed different expression patterns among different tissues and cultivars of L. chinense var. rubrum. Many LcbHLHs were expressed in the leaves, and only a few genes were highly expressed in the flowers. Six LcbHLHs candidate genes were identified by bioinformatics analysis and expression analysis. Further Real-time quantitative PCR analysis and protein interaction network analysis showed that LcbHLH156, which is one of the candidate proteins belonging to the IIIf subfamily, could interact with proteins related to anthocyanin synthesis. Therefore, LcbHLH156 was transiently expressed in L. chinense var. rubrum to verify its function in regulating anthocyanin synthesis. Compared with the control group, red pigment accumulation appeared at the wound after injection, and the total anthocyanin content increased at the wound of leaves. These results lay a foundation for the research of the regulation mechanism of leaf colors in L. chinense var. rubrum and also provide a basis for the function of the LcbHLH family.
Hepatoprotective Effects of Four Brazilian Savanna Species on Acetaminophen-Induced Hepatotoxicity in HepG2 Cells
Authors: Ribeiro Gislane dos Santos , Martins Diegue Henrique Nascimento , Gomes João Victor Dutra , Davies Noel William , Fagg Christopher William , Simeoni Luiz Alberto , Homem-de-Mello Mauricio , Magalhães Pérola Oliveira , Silveira Dâmaris , Fonseca-Bazzo Yris Maria
Published: September 2023

We investigated four Cerrado plant species, i.e., Cheiloclinium cognatum (Miers) A.C.Sm, Guazuma ulmifolia Lam., Hancornia speciosa Gomes, and Hymenaea stigonocarpa Mart. ex Hayne, against acetaminophen toxicity using an in vitro assay with HepG2 cells. The activity against acetaminophen toxicity was evaluated using different protocols, i.e., pre-treatment, co-treatment, and post-treatment of the cells with acetaminophen and the plant extracts. HepG2 cell viability after treatment with acetaminophen was 39.61 ± 5.59% of viable cells. In the pre-treatment protocol, the extracts could perform protection with viability ranging from 50.02 ± 15.24% to 78.75 ± 5.61%, approaching the positive control silymarin with 75.83 ± 5.52%. In the post-treatment protocol, all extracts and silymarin failed to reverse the acetaminophen damage. In the co-treatment protocol, the extracts showed protection ranging from 50.92 ± 11.14% to 68.50 ± 9.75%, and silymarin showed 77.87 ± 4.26%, demonstrating that the aqueous extracts of the species also do not increase the toxic effect of acetaminophen. This protection observed in cell viability was accompanied by a decrease in ROS. The extracts’ hepatoprotection can be related to antioxidant compounds, such as rutin and mangiferin, identified using HPLC-DAD and UPLC-MS/MS. The extracts were shown to protect HepG2 cells against future APAP toxicity and may be candidates for supplements that could be used to prevent liver damage. In the concomitant treatment using the extracts with APAP, it was demonstrated that the extracts do not present a synergistic toxicity effect, with no occurrence of potentiation of toxicity. The extracts showed considerable cytoprotective effects and important antioxidant characteristics.
Heterologous Expression of OtsB Increases Tuber Yield and Phenotypic Stability in Potato under Both Abiotic and Biotic Stresses
Authors: Morgan Britany Lauren , Kakeshpour Tayebeh , Occhialini Alessandro , King Gabriella , Sichterman Megan , Harbison Stacee A. , Rigoulot Stephen B. , Brabazon Holly , Stewart Charles Neal , Lenaghan Scott C.
Published: September 2023

Climate-smart and sustainable crops are needed for the future. Engineering crops for tolerance of both abiotic and biotic stress is one approach. The accumulation of trehalose, controlled through trehalose-6-phosphate synthase (TPS) or OtsA and trehalose-6-phosphate phosphatase (TPP) or OtsB genes in microbes, is known to provide protection for many microbial and fungal species against abiotic stress. The effect of trehalose accumulation in plant species is less understood. Here, we studied the heterologous expression of Escherichia coli OtsB in potato (Solanum tuberosum var. ‘Desiree’) with regards to stress tolerance. The performance of transgenic lines was assessed in both growth chambers and greenhouse mesocosms. Overexpressing potato OtsB lines significantly increased resilience to heat, photoperiod, herbivory, and competition when compared with wildtype plants. Most strikingly, when subjected to high temperatures, transgenic lines exhibited a significantly lower reduction in tuber yield ranging from 40% to 77%, while wildtype plants experienced a 95% decrease in tuber yield. When exposed to competitors in a selected StSP3D::OtsB line, tuber yield was 1.6 times higher than wildtype. Furthermore, transgenic lines performed significantly better under low-nutrient regimes: under competition, yield increased by 1.5-fold. Together, these results demonstrate that increased trehalose has the potential to create more resistant and stable crop plants.
Selection and Characterization of Phosphate-Solubilizing Fungi and Their Effects on Coffee Plantations
Authors: Arias Rosa María , Heredia Abarca Gabriela , del Carmen Perea Rojas Yamel , de la Cruz Elizondo Yadeneyro , García Guzman Karla Yosselín
Published: September 2023

The use of phosphate-solubilizing fungi in coffee cultivation is an alternative to the use of traditional fertilizers. The objective of this study was to analyze the mechanisms involved in the phosphorus solubilization of fungal strains and to evaluate the effect of a phosphate-solubilizing strain on coffee plants. For this, phosphorus-solubilizing fungal strains were selected for evaluation of their solubilization potential and phosphatase activity. Coffee plants were inoculated in the field with a phosphate-solubilizing strain, and the soil and foliar soluble phosphorus contents, as well as coffee bean yield, were quantified. Of the 151 strains analyzed, Sagenomella diversispora, Penicillium waksmanii, and Penicillium brevicompactum showed the highest solubilization. Aspergillus niger and P. waksmanii presented the highest soluble phosphorus values; however, P. brevicompactum showed the highest phosphatase activity. The P. brevicompactum strain inoculated on the coffee plants did not favor the foliar phosphorus content but increased the soil soluble phosphorus content in two of the coffee plantations. The plants inoculated with the phosphate-solubilizing strain showed an increase in coffee bean weight on all plantations, although this increase was only significant in two of the three selected coffee plantations.
Sulla (Hedysarum coronarium L.) Response to Drought Stress during Early Vegetative Stage
Authors: Rossi Roberta , Amato Mariana , Claps Salvatore
Published: September 2023

Sulla (Hedysarum coronarium L.) is a Mediterranean biannual anthelmintic forage. Due to its high productivity, nutraceutical value, and suitability for harsh environments, interest in this crop is growing. Under the current scenario of climate change and water scarcity, it is important to evaluate crop drought tolerance, especially for newly bred materials. Drought stress and well-watered conditions (50 vs. 80% of the field capacity) were applied in a pot experiment to compare responses of the widespread commercial variety Bellante with those of a recently released variety named ‘Centauro’, currently registered in the Italian national register of plant varieties but not yet available on the market. Compared to the well-watered treatment, drought-stressed plants showed lower values of fresh biomass (−69%), number of leaves (−68%), and root length (−49%). The Centauro cv. showed a different architecture to Bellante with more shoots (+43% P < 0.05) and a trend for more leaves (+25% P = 0.08). These traits are possibly related to its superior palatability. Centauro also developed a higher root length (+70%, P < 0.05) across irrigation levels. Drought stress affected condensed tannin (CT) content. A significant genotype × environment interaction was found with Centauro displaying more (+50%) and less (−35%) CT than Bellante under drought stress and well-watered conditions, respectively. The higher constitutive root length density of Centauro may be exploited in breeding programs aimed at improving the root sink, given the role of this trait in resource acquisition capacity and root-derived ecosystem services.
Unveiling the Secrets of Calcium-Dependent Proteins in Plant Growth-Promoting Rhizobacteria: An Abundance of Discoveries Awaits
Authors: Agaras Betina Cecilia , Grossi Cecilia Eugenia María , Ulloa Rita María
Published: September 2023

The role of Calcium ions (Ca2+) is extensively documented and comprehensively understood in eukaryotic organisms. Nevertheless, emerging insights, primarily derived from studies on human pathogenic bacteria, suggest that this ion also plays a pivotal role in prokaryotes. In this review, our primary focus will be on unraveling the intricate Ca2+ toolkit within prokaryotic organisms, with particular emphasis on its implications for plant growth-promoting rhizobacteria (PGPR). We undertook an in silico exploration to pinpoint and identify some of the proteins described in the existing literature, including prokaryotic Ca2+ channels, pumps, and exchangers that are responsible for regulating intracellular Calcium concentration ([Ca2+]i), along with the Calcium-binding proteins (CaBPs) that play a pivotal role in sensing and transducing this essential cation. These investigations were conducted in four distinct PGPR strains: Pseudomonas chlororaphis subsp. aurantiaca SMMP3, P. donghuensis SVBP6, Pseudomonas sp. BP01, and Methylobacterium sp. 2A, which have been isolated and characterized within our research laboratories. We also present preliminary experimental data to evaluate the influence of exogenous Ca2+ concentrations ([Ca2+]ex) on the growth dynamics of these strains.
Shrinkage Compensation and Effect of Building Orientation on Mechanical Properties of Ceramic Stereolithography Parts
Authors: Arora Piyush , Mostafa Khaled G. , Russell Emmanuel , Dehgahi Shirin , Butt Sajid Ullah , Talamona Didier , Qureshi Ahmed Jawad
Published: September 2023

Stereolithography additive manufacturing (SLA-AM) can be used to produce ceramic structures by selectively curing a photosensitive resin that has ceramic powder in it. The photosensitive resin acts as a ceramic powder binder, which is burned, and the remaining ceramic part is sintered during post-processing using a temperature–time-controlled furnace. Due to this process, the ceramic part shrinks and becomes porous. Moreover, additive manufacturing leads to the orthotropic behavior of the manufactured parts. This article studies the effect of the manufacturing orientation of ceramic parts produced via SLA-AM on dimensional accuracy. Scaled CAD models were created by including the calculated shrinkage factor. The dimensions of the final sintered specimens were very close to the desired dimensions. As sintering induces porosity and reduces the mechanical strength, in this study, the effect of orientation on strength was investigated, and it was concluded that the on-edge specimen possessed by far the highest strength in terms of both compression and tension.
Investigation of the Mechanical and Tribological Behavior of Epoxy-Based Hybrid Composite
Authors: Albahkali Thamer , Fouly Ahmed , Alnaser Ibrahim A. , Elsheniti Mahmoud B. , Rezk Ahmed , Abdo Hany S.
Published: September 2023

The main target of this study is to evaluate the impact of hybrid reinforcement using Al2O3 nanoparticles and graphite on the epoxy nanocomposites’ mechanical and tribological properties. Various weight fractions of the reinforcement materials, ranging from 0 to 0.5 wt.%, were incorporated into the epoxy. The aim is to enhance the characteristics and durability of the polymers for potential utilization in different mechanical applications. The addition of hybrid additives consisting of Al2O3 nanoparticles and graphite to the epoxy resin had a noticeable effect on the performance of the epoxy nanocomposites. The incorporation of these additives resulted in increased elasticity, strength, toughness, ductility, and hardness as the concentration of reinforcement increased. The enhancement in the stiffness, mechanical strength, toughness and ductility reached 33.9%, 25.97%, 25.3% and 16.7%, respectively. Furthermore, the frictional tests demonstrated a notable decrease in both the coefficient of friction and wear with the rise of the additives’ weight fraction. This improvement in the structural integrity of the epoxy nanocomposites led to enhanced mechanical properties and wear resistance. The SEM was utilized to assess the surfaces of tested samples and provide insights into the wear mechanism.
Investigation of Polymer-Assisted CO2 Flooding to Enhance Oil Recovery in Low-Permeability Reservoirs
Authors: Chen Xin , Li Yiqiang , Sun Xiaoguang , Liu Zheyu , Liu Jianbin , Liu Shun
Published: September 2023

CO2 flooding is a favorable technical means for the efficient development of low-permeability reservoirs, and it can also contribute to the realization of net-zero CO2 emissions. However, due to the unfavorable viscosity ratio and gravity overriding effect, CO2 channeling will inevitably occur, seriously affecting its storage and displacement effects. This paper conducts a systematic study on the application of polymer-assisted CO2 flooding in low-permeability reservoirs. Firstly, the polymer agent suitable for low-permeability reservoirs is optimized through the viscosity-increasing, rheological, and temperature- and salt-resistant properties of the solution. Then, the injectivity performance, resistance-increasing ability, and profile-improving effect of the polymer solution were evaluated through core experiments, and the optimum concentration was optimized. Finally, the enhanced oil recovery (EOR) effects of polymer-assisted and water-assisted CO2 flooding were compared. The results show that the temperature-resistant polymer surfactant (TRPS) has a certain viscosity-increasing performance, good temperature resistance performance, and can react with CO2 to increase the solution viscosity significantly. Meanwhile, TRPS has good injection performance and resistance-increasing effect. The resistance increasing factor (η and η′) of TRPS-assisted CO2 flooding increases with increased permeability, the concentration of TRPS solution, and injection rounds. Considering η′ and the profile improvement effect comprehensively, the application concentration of TRPS should be 1000 mg/L. The EOR effect of TRPS-assisted CO2 flooding is 8.21% higher than that of water-assisted CO2 flooding. The main effective period is in the first and second rounds, and the best injection round is three. The research content of this paper can provide data support for the field application of polymer-assisted CO2 flooding in low-permeability reservoirs.
The Highly Enhanced Efficiency of the Photocatalytic Reduction of CO2 over Bi2WO6 Nanosheets by NaOH Microregulation
Authors: Song Chao , Sun Yangang , Zhang Li , Liu Shuang , Wang Jinguo , An Wei , Men Yong , Yan Zhenrong
Published: September 2023

The photoreduction of CO2 to other products containing carbon through simulated photosynthesis is a promising area of research. However, given the complexity of the CO2 photocatalytic reduction reaction, it is crucial to adjust the structure of the photocatalysts. The focus of this study was on creating NaOH-modified Bi2WO6 nanosheet photocatalysts via a one-step hydrothermal route and using them to convert CO2 into CO through photocatalytic reduction under the condition of not using an electron sacrifice agent. The results of characterizations and activity data showed that adding an appropriate amount of NaOH significantly improved the photoreduction activity of CO2, as seen in the BWO-2 catalyst. The efficiency of photocatalysts could be improved by tuning the band structure through the addition of an appropriate amount of alkali. This adjustment improves the separation of photogenerated carriers and controls the concentration of oxygen vacancy to reduce recombination. As a result, the photocurrent activity is highly enhanced, leading to better reduction performance compared to unmodified photocatalysts. In experiments, the CO yield of the modified photocatalyst BWO-2 remained above 90 μmol/g after four trials, indicating its effectiveness in reducing CO2. This study offers insights into the regulation of band structure in bismuth-based photocatalysts for efficient CO2 reduction.
Experimental Study on Permeability Characteristics of Mudstone under High Temperature Overburden Condition
Authors: Ma Jian , Zhang Yunlong , Lv Jiakun , Yu Kun
Published: September 2023

High-temperature treatment significantly impacts the permeability of mudstone. The permeability of mudstone after exposure to high temperatures is closely influenced by the temperature it experiences and the stress state it is subjected to. This study examines the change in macroscopic physico-mechanical properties of mudstone with temperature following high-temperature treatment. Additionally, we conducted experimental research on the gas and water seepage behavior of mudstone specimens from the top of the coal seam of Taiyuan Group–Shanxi Group in the Ordos Basin. The coal-rock mechanics-permeability test system TAWD-2000 was employed for this purpose. Subsequently, we analyzed the evolution of mudstone permeability after high-temperature treatment with consideration to temperature, axial pressure, and other influencing factors. The findings reveal that gas permeability of mudstone gradually increases with increasing temperature, while water permeability initially decreases and subsequently increases. Furthermore, both gas and water permeability of mudstone exhibit a trend of decreasing and then increasing with rising stress levels after undergoing the same high-temperature treatment. We constructed a quadratic mathematical model with a goodness of fit of 99.4% and 89.2% to describe the relationship between temperature–stress coupling and mudstone gas and water permeability. This model underscores the significance of temperature–stress coupling on mudstone permeability and provides valuable guidance for numerically calculating the gas–water transport law of peripheral rock in the underground coal gasification process and its practical application in engineering.
Physical Properties of Hydrocarbon Source Reservoir in the Lucaogou Formation in Junggar Basin (China) and Its Influence on Adsorption Ability and Surface Free Energy
Authors: Zou Shuangying , Yao Peng , Wang Dongdong , Zhang Xiaoyang , Ge Dongfeng , Chen Yongmei , Zhang Junjian , Chen Huafei , Kong Fandu , Liu Zhu , Shao Yinchuan
Published: September 2023

A physical property of a shale gas reservoir affects shale gas content, then restricts the shale gas resource potential. In this paper, lithofacies and spatial distribution of the southern margin of the Junggar Basin in Xinjiang Province are identified and occurrence strata, gas content, and reservoir properties of shale gas are studied. Based on adsorption potential theory, adsorption and surface free energy of all the sample is discussed. The conclusions are as follows. (1) All the shale samples can be divided into five lithological phases. For example, black oil shale/shale (lithology) phase and gray-black-gray/dolomite mudstone (lithology) phase are the most developed; compared with the middle and lower sections, the vertical development continuity of the upper hydrocarbon source rock is better. Lithology of this section is mainly composed of shale mixed with marlstone and dolomite interlayer. From the horizontal view of this section, the overall trend is gradually thickening from west to east and from north to south. (2) Semi-deep lake-phase is the most developed, indicating a decreasing trend of thickness from sedimentary center to surrounding strata. (3) Sedimentary period of Lucaogou Formation is a deep water area of the lake basin; then, the TOC content is affected by the sedimentary environment. As sedimentary water depth increases, TOC content will increase, which results in the highest TOC content in the area. A specific surface area is roughly positively correlated with porosity, clay mineral content, and percentage of illite/montmorillonite interlayer ratio and negatively correlated with TOC content. (4) During the adsorption process, adsorption potential decreases with an increase in equilibrium pressure, and adsorption space increases with an increase in equilibrium pressure. Maximum adsorption space of all the sample were 0.20~0.25 cm3·g−1; then, its value is larger than the maximum adsorption space of other coal samples (0.025~0.20 cm3·g−1). In the same adsorption space, the corresponding adsorption potential of the coal sample is much larger than that of other samples. The reason is that the porosity permeability of this sample is relatively larger, leading to its better physical properties.
Christianity and Fraternal Globalisation
Authors: de Aviz Darlan Aurélio , Rodrigues Luís M. Figueiredo
Published: September 2023

This paper discusses the importance of fraternity in building more fair and humane societies. The globalised world poses new challenges to the understanding of fraternity, especially when it is seen as an attack on the dignity of each person. Religions are considered to have an important role to play in responding to the dehumanisation that a certain type of globalisation can promote. To this end, we reflect on the threats and opportunities that the globalised world offers and how religious thought can make a positive contribution. Based on Pope Francis’ challenges, we will respond to what the “something greater” is that fraternity has to offer the world. The answer is developed using sources from the sacred scripture and the patristics to understand Christianity, where it is concluded that the model of relationships between people, in the thought of the magisterium in the light of sacred scripture, aims to awaken the awareness of a social friendship, which will grow to the extent that one offers what is best for the other, simply by loving them and being who they are.
Waste Management in the Smart City: Current Practices and Future Directions
Authors: Szpilko Danuta , de la Torre Gallegos Antonio , Jimenez Naharro Felix , Rzepka Agnieszka , Remiszewska Angelika
Published: September 2023

The discourse surrounding sustainability, particularly in the urban environment, has gained considerable momentum in recent years. The concept of a smart city epitomises the integration of innovative technological solutions with community-centred approaches, thereby laying the groundwork for a sustainable lifestyle. One of the crucial components of this integration is the effective and innovative management of waste. The aim of this article was to classify scientific research pertaining to waste management within the context of smart city issues, and to identify emerging directions for future research. A systematic literature review, based on a bibliometric analysis of articles included in the Scopus and Web of Science databases, was conducted for this study. The purpose of such a systematic review is to identify, integrate, and evaluate research on a selected topic, using clearly defined criteria. The research query included: TITLE-ABS-KEY (“smart city” AND (waste OR garbage OR trash OR rubbish)) in the case of Scopus, and TS = (“smart city” AND (waste OR garbage OR trash OR rubbish)) in the case of the Web of Science database. A total of 1768 publication records qualified for the analysis. This study presents an investigation into the current and forthcoming directions of waste management in smart cities, synthesising the latest advancements and methods. The findings outline specific future research directions encompassing technological advancement, special waste challenges, digitisation, energy recovery, transportation, community engagement, policy development, security, novel frameworks, economic and environmental impact assessment, and global implications. These insights reflect a multifaceted approach, advocating a technology-driven perspective that is integral to urban sustainability and quality of life. The study’s findings provide practical avenues for cities to enhance waste management through modern technologies, promoting efficient systems and contributing to sustainable urban living and the circular economy. The insights are vital for policymakers and industry leaders globally, supporting the creation of universal standards and policies, thereby fostering comprehensive waste management systems aligned with global sustainability objectives.
A Combination of OBIA and Random Forest Based on Visible UAV Remote Sensing for Accurately Extracted Information about Weeds in Areas with Different Weed Densities in Farmland
Authors: Feng Chao , Zhang Wenjiang , Deng Hui , Dong Lei , Zhang Houxi , Tang Ling , Zheng Yu , Zhao Zihan
Published: September 2023

Weeds have a significant impact on the growth of rice. Accurate information about weed infestations can provide farmers with important information to facilitate the precise use of chemicals. In this study, we utilized visible light images captured by UAVs to extract information about weeds in areas of two densities on farmland. First, the UAV images were segmented using an optimal segmentation scale, and the spectral, texture, index, and geometric features of each segmented object were extracted. Cross-validation and recursive feature elimination techniques were combined to reduce the dimensionality of all features to obtain a better feature set. Finally, we analyzed the extraction effect of different feature dimensions based on the random forest (RF) algorithm to determine the best feature dimensions, and then we further analyzed the classification result of machine learning algorithms, such as random forest, support vector machine (SVM), decision tree (DT), and K-nearest neighbors (KNN) and compared them based on the best feature dimensions. Using the extraction results of the best classifier, we created a zoning map of the weed infestations in the study area. The results indicated that the best feature subset achieved the highest accuracy, with respective overall accuracies of 95.38% and 91.33% for areas with dense and sparse weed densities, respectively, and F1-scores of 94.20% and 90.57. Random forest provided the best extraction results for each machine learning algorithm in the two experimental areas. When compared to the other algorithms, it improved the overall accuracy by 1.74–12.14% and 7.51–11.56% for areas with dense and sparse weed densities, respectively. The F1-score improved by 1.89–17.40% and 7.85–10.80%. Therefore, the combination of object-based image analysis (OBIA) and random forest based on UAV remote sensing accurately extracted information about weeds in areas with different weed densities for farmland, providing effective information support for weed management.