bluuebunny/crossref_metadata_2025 huggingface dataset:
Preparation of Indomethacin Co-Crystals; Comparison of XRD, THz, and FT-IR Spectral Analyses; and Enhancement of Solubility
Authors: Hsu Chih-Chin , Hung Chih-Tse , Lin Ya-Hsuan , Tsai Hua-Jeng , Hu Po-Chih , Lin Yi-Ping , Chen Jyh-Chern , Hsu Shen-Fu , Hsieh Hsyue-Jen
Published: July 2024

Background: The aqueous solubility of indomethacin, a poorly water-soluble anti-inflammatory drug, was enhanced by co-crystallization with co-formers. The co-crystals were characterized and compared by an X-ray diffraction (XRD) analysis, terahertz (THz) spectroscopy, and Fourier transform infrared (FT-IR) spectroscopy. Methods: Indomethacin co-crystals with either amides (saccharin, nicotine amide, and urea) or amino acids (lysine and histidine) as co-formers were prepared through the solvent evaporation method. The co-crystals were characterized by XRD, THz, and FT-IR analyses, followed by solubility tests to examine the solubility enhancement. Results: Both the XRD and THz analyses were capable of distinguishing co-crystals from physical mixtures; however, the THz spectra were relatively simpler and clearer than the XRD analysis. Furthermore, the solubility of indomethacin was successfully increased by two to three times that of pure indomethacin after co-crystallization with the above five co-formers. Conclusion: Five kinds of indomethacin co-crystals (with enhanced solubility) were successfully prepared and confirmed by the three spectroscopy techniques, XRD, THz, and FT-IR. The identification of co-crystals was achieved by a THz analysis, giving relatively simpler and clearer spectra with less noise. Hence, in addition to an XRD analysis, a THz analysis (a non-destructive, non-ionizing radiative, and relatively rapid measurement technique which is convenient and safe to use) is a good alternative method to characterize co-crystals.
Regulations and Fintech: A Comparative Study of the Developed and Developing Countries
Authors: Vijayagopal Preethi , Jain Bhawana , Ayinippully Viswanathan Shyam
Published: July 2024

Financial technology (Fintech) has influenced business by helping create better services for consumers and businesses. Fintech, however, brings new challenges for regulators, who struggle to keep pace with the constant evolution of technology and the resulting disruption. The progress of technology and regulations in the Fintech industry has been uneven across developed and developing countries, resulting in numerous opportunities and challenges. Considerable progress has recently been made in the adoption of Fintech and the subsequent development and implementation of regulations in the US, the UK, and India. While the United States (US) and the United Kingdom (UK) are global leaders in Fintech innovation, India has shown fast-paced growth in adopting and utilizing Fintech services. This paper examines the growth and evolution of Fintech in the US, the UK, and India and also explores how the regulatory agencies across these countries have responded to the evolution of Fintech. This paper finds that economies should work towards improving digital infrastructure, financial inclusion, and financial literacy and enhance the collaboration among regulators, Fintech firms, and other stakeholders.
The Impact of Social Capital on Community Resilience: A Comparative Study of Seven Flood-Prone Communities in Nanjing, China
Authors: Chen Yi , Liu Hui , Lin Shuchang , Wang Yueping , Zhang Qian , Feng Liaoling
Published: July 2024

Social capital plays a crucial role in enhancing community resilience during flood disasters. This study investigates the influence of social capital on community resilience in Nanjing, China. Social capital is composed of five aspects: cohesion, collective efficacy, sense of belonging, trust and reciprocity and informal social control. Factor analysis and multiple regression analysis are employed to analyze the dimensions of social capital and its impact on community resilience. Our results demonstrate that social cohesion and collective efficacy are the most representative factors of social capital. Reformed housing communities typically have higher cohesion than those in commercial and affordable housing. Affordable housing communities in flood-prone areas have higher collective efficacy but lower trust and reciprocity. Commercial housing communities have higher informal social control but have great internal differences in collective efficacy. We strongly urge government decision makers to enhance flood resilience by fostering social capital within local communities.
Unveiling the Potential: Remote Monitoring and Telemedicine in Shaping the Future of Heart Failure Management
Authors: Liu Ju-Chi , Cheng Chun-Yao , Cheng Tzu-Hurng , Liu Chen-Ning , Chen Jin-Jer , Hao Wen-Rui
Published: July 2024

Heart failure (HF) remains a significant burden on global healthcare systems, necessitating innovative approaches for its management. This manuscript critically evaluates the role of remote monitoring and telemedicine in revolutionizing HF care delivery. Drawing upon a synthesis of current literature and clinical practices, it delineates the pivotal benefits, challenges, and personalized strategies associated with these technologies in HF management. The analysis highlights the potential of remote monitoring and telemedicine in facilitating timely interventions, enhancing patient engagement, and optimizing treatment adherence, thereby ameliorating clinical outcomes. However, technical intricacies, regulatory frameworks, and socioeconomic factors pose formidable hurdles to widespread adoption. The manuscript emphasizes the imperative of tailored interventions, leveraging advancements in artificial intelligence and machine learning, to address individual patient needs effectively. Looking forward, sustained innovation, interdisciplinary collaboration, and strategic investment are advocated to realize the transformative potential of remote monitoring and telemedicine in HF management, thereby advancing patient-centric care paradigms and optimizing healthcare resource allocation.
Genetic Differences between Male and Female Pattern Hair Loss in a Korean Population
Authors: Lee Jihyun , Choi Ja-Eun , Ha Joohun , Kim Youngjoo , Lee Changhyun , Hong Kyung-Won
Published: July 2024

Studies on androgenetic alopecia (AGA or patterned hair loss (PHL)) have suggested different underlying pathological mechanisms between males and females. While many genetic factors for male hair loss have been identified through genome-wide association studies (GWASs), the genetic determinants of female hair loss remain unclear. In this study, we analyzed approximately 1000 individuals (436 males and 568 females) to identify sex-specific genetic factors. We conducted three independent GWASs for the total, male-only, and female-only groups, identifying three novel loci (rs7814359, rs2163085, and rs4793158 of the TSNARE1, FZD1, and GJC1 genes, respectively). rs7814359 showed a significant genome-wide association with AGA in the combined sex group and a weak association in both the male-only and female-only groups. The single nucleotide polymorphism (SNP) rs2163085 showed a significant genome-wide association with AGA in the combined group and notable significance in females. The rs4793158 SNP showed a suggestive association with AGA in both the combined and female-only groups. TSNARE1, related to rs7814359, is involved in vesicle transport. FZD1 is a key regulator of the Wnt signaling pathway. GJC1 is a gap junction protein. The associations of FZD1 and GJC1 with female-specific AGA suggest that sex hormones, such as estrogen, may influence FPHL through these genes. These findings will contribute to our understanding of the sex-specific pathophysiology of AGA.
Phosphonic Acids as Corrosion Inhibitors and Adhesion Promoters for Organic Coatings and Bronze
Authors: Mikić Dajana , Radovanović-Perić Floren , Otmačić Ćurković Helena
Published: July 2024

Currently used organic coatings for the protection of bronze sculptures have a relatively short lifespan as a consequence of strict requirements of conservation ethics, which limit the selection of coatings. For that reason, enhancement of the corrosion protection level and durability of appropriate coatings is needed. The aim of this work was to examine if corrosion protection of bronze by selected acrylic and polyurethane coatings could be improved by using two phosphonic acids, 16-phosphonohexadecanoic acid (COOH-PA) and 12-aminododecylphosphonic acid (NH2-PA). Electrochemical measurements (linear polarization and electrochemical impedance spectroscopy, EIS) were performed to gain an insight into the influence of these phosphonic acids on the performance of the coatings during a two-week exposure to artificial acid rain and a three-month outdoor exposure. Besides the influence on the corrosion protection level, the influence on the coating adhesion was examined as well. A pull-off test clearly confirmed that the studied phosphonic acids act as adhesion promoters of both polyurethane and acrylic coatings, while electrochemical studies revealed improvements in corrosion protection levels, especially in the case of the acrylic coating Paraloid B72.
Additional Rehabilitative Robot-Assisted Gait Training for Ambulation in Geriatric Individuals with Guillain–Barré Syndrome: A Case Report
Authors: Chen Fred Yi-Shueh , Hou Wen-Hsuan , Lee Hsun-Hua , Huang Ying-Chi , Siow Co Yih
Published: July 2024

We present a case of a 75-year-old Asian woman with Guillain–Barré syndrome (GBS) who underwent a 1-month comprehensive rehabilitation training program supplemented by robot-assisted gait training (RAGT). GBS can lead to fatigue and prolonged bed rest, thereby further debilitating older patients. Although exercise intervention is recommended for GBS, a consensus regarding the appropriate intensity has yet to be established. Individualized strategies are required because older patients experience varying levels of fatigue and frailty. We used a technological adjunct to support comprehensive rehabilitation for GBS reconditioning in an older patient. To the best of our knowledge, research involving the use of an exoskeleton robotic device in the geriatric population with GBS is limited. Our case demonstrates the feasibility and safety of RAGT for improving lower limb muscle power and scores on the Barthel Index, Clinical Frailty Scale, and Instrumental Activities of Daily Living Scale at discharge from a geriatric ward.
The Effects of Three Antibacterial Nanoparticle Coatings on the Surface Characteristics of Stainless Steel
Authors: Al-Mayali Ahmed , Kadhum Ammar , Alzubaydi Thair
Published: July 2024

The aim of this study is to investigate the antibacterial capabilities of different coating durations of three nanoparticle (NP) coatings: molybdenum (Mo), tantalum (Ta), and zinc oxide (ZnO), and their effects on the surface characteristics of 316L stainless steel (SS). The coated substrates underwent characterization utilizing field emission scanning electron microscopy (FE-SEM), energy dispersive X-ray spectrometry (EDX), and X-ray diffractometer (XRD) techniques. The antibacterial efficacy of NPs was evaluated using the agar diffusion method. The FE-SEM and EDX images confirmed the presence of nano-sized particles of Mo, Ta, and ZnO on the surface of the substrates with perfectly symmetrical spheres and a uniform distribution of the NPs. All groups demonstrated antibacterial activity, and the ability to inhibit the growth of Streptococcus mutans and Lactobacillus acidophilus bacteria. The ZnO group had the most potent antibacterial effect, followed by the Mo group, while the Ta group had the least effect. A direct-current (DC) plasma sputtering system was used to produce nano-coatings of high purity that were homogeneous, crack-free and showed no sign of delamination. Bacterial strains exposed to Mo, Ta, and ZnO coated surfaces exhibited a significant loss of viability in a time-dependent manner. The optimum sputtering time to ensure the best antibacterial properties and preserve the resources was 1 hour (h) for Mo, 3 h for Ta and 6 h for ZnO.
Reduction in Porosity in GMAW-P Welds of CP780 Galvanized Steel with ER70S-3 Electrode Using the Taguchi Methodology
Authors: García-Gómez Maleni , Curiel-López Francisco Fernando , Taha-Tijerina José Jaime , López-Morelos Víctor Hugo , Verduzco-Juárez Julio César , García-Ochoa Carlos Adrián
Published: July 2024

In this study, the theoretical welding parameters influencing porosity formation were examined with the aim of reducing or minimizing porosity levels. An experimental design was implemented using the Taguchi methodology for data analysis, resulting in an L9 orthogonal array matrix of experiments. The welding variables considered in the orthogonal array were peak current, peak time, and frequency. Nine lap welds were performed on CP780 steel using the gas metal arc welding process with pulsed arc (GMAW-P), employing an ER70S-3 electrode as filler metal. The percentage of porosity was determined as a response variable, and the actual heat input was treated as a covariable, thereby identifying the welding parameters with the predicted values. Three repetitions were conducted with the optimal welding parameters to validate the Taguchi prediction. The quality of the welds was assessed through radiographic inspection, and metallographic preparation was performed, revealing the microstructure with 5% Nital for 12 s. The samples were analyzed using an optical microscope, and images were obtained with the collage technique. The results showed that the welding parameters predicted by the Taguchi statistics were favorable for all three predicted welded joints. The maximum percentage of porosity obtained was 19%, which was reduced to 1% using the Taguchi methodology, demonstrating the effectiveness of this statistical tool for process optimization. It was observed that for heat input values of 230 to 250 J/mm, the presence of porosities is dramatically reduced, finding a very small window that allows the gases generated by the burning of zinc to be expelled to the surface.
A Study on Powder Metallurgy Process for x Electric Vehicle Stator Core
Authors: Kim Jaemin , Lee Seonbong
Published: July 2024

The powder metallurgy process of manufacturing the motor core and inductor core using SMC greatly changes formability depending on the process variables. Therefore, this study explored the optimal process conditions of the powder metallurgy of the SMC stator core using Fe-6.5 wt.%Si by applying the Taguchi method, and selected deviations between the maximum and minimum relative densities as characteristic values; selected the formation pressure, molding temperature, and heating time as control factors; and derived the process conditions with the maximum SNR. As a result, the molding pressure was 120 MPa, the molding temperature was 500 °C, and the heating time was 120 s, and the material properties of the electrical properties’ core loss, saturation flux density, and bulk conductivity were measured and analyzed. After that, a prototype was produced, the analysis was verified, the mechanical properties were verified by performing density and SEM analysis at 15, 9, and 3 mm points based on the press vertical direction, and a motor was manufactured to verify the electrical properties.
The Modification of Aluminum Oxide Inclusions in Bearing Steel under Different Cleanliness Conditions by Rare Earth Elements
Authors: Wang Weining , Xia Wenzhi , Zhou Yun , Deng Aijun , Bao Guangda , Liao Zhiyou , Wang Haichuan
Published: July 2024

The impact of rare earth treatment on the chemical morphology evolution of non-metallic inclusions in bearing steel under different initial cleanliness conditions was studied through simulation. Thermodynamic calculations indicate that at an oxygen content of 0.001%, the evolution route of inclusions with increasing Ce content was Al2O3 → CeAl11O18 + CeAlO3 → CeAlO3 + Ce2O2S → Ce2O2S → Ce2O2S + CeS. As the initial oxygen content decreases, the proportion of CeAlO3 decreases, leading to easier conversion of CeAlO3 to Ce2O2S. Vacuum induction furnace experiments demonstrated that with an oxygen content of 0.001%, an increase in Ce content results in a gradual rise in the proportion of inclusions in steel sized 1~2 μm. In contrast, the proportion of inclusions sized 2~5 μm decreases. Consequently, the overall content of inclusions in steel decreases, along with a reduction in both the number density and average size of inclusions. Introducing bearing steel melt with approximately 0.01% Ce rare earth to bearing steel with initial oxygen contents of 0.0005%, 0.001%, and 0.0015% showed an evolution of inclusions from Ce2O2S and CeS to Ce2O3, CeAlO3, etc. The average inclusion size significantly increased from 0.7 μm to 2.16 μm. Morphologically, the transition of inclusions from precipitated to polymerized forms occurred as the initial oxygen content rose. High-temperature laser confocal microscopy experiments demonstrated that inclusions in low cleanliness conditions tend to agglomerate more than those in high cleanliness conditions, contributing to the increase in average size.
From Hypothalamic Obesity to Metabolic Dysfunction-Associated Steatotic Liver Disease: Physiology Meets the Clinics via Metabolomics
Authors: Lonardo Amedeo , Weiskirchen Ralf
Published: July 2024

Metabolic health is tightly regulated by neuro-hormonal control, and systemic metabolic dysfunction may arise from altered function of the hypothalamic–anterior pituitary axis (HAPA). Ancient experimental observations of hypothalamic obesity (HO) and liver cirrhosis occurring among animals subjected to hypothalamic injury can now be explained using the more recent concepts of lipotoxicity and metabolic dysfunction-associated steatotic liver disease (MASLD). Lipotoxicity, the range of abnormalities resulting from the harmful effects of fatty acids accumulated in organs outside of adipose tissue, is the common pathogenic factor underlying closely related conditions like hypothalamic syndrome, HO, and MASLD. The hormonal deficits and the array of metabolic and metabolomic disturbances that occur in cases of HO are discussed, along with the cellular and molecular mechanisms that lead, within the MASLD spectrum, from uncomplicated steatotic liver disease to steatohepatitis and cirrhosis. Emphasis is placed on knowledge gaps and how they can be addressed through novel studies. Future investigations should adopt precision medicine approaches by precisely defining the hormonal imbalances and metabolic dysfunctions involved in each individual patient with HO, thus paving the way for tailored management of MASLD that develops in the context of altered HAPA.
Editorial for the Special Issue on Wearable and Implantable Bio-MEMS Devices and Applications
Authors: Ji Bowen , Gao Kunpeng
Published: July 2024

Wearable and implantable bio-MEMS sensors and actuators have attracted tremendous attention in the fields of health monitoring, disease treatment, and human–machine interaction, to name but a few [...]
LiDonit®—A Potential Secondary Raw Material for Ceramic Applications in Concentrated Solar Energy
Authors: Alkan Gözde , Mechnich Peter , Pernpeintner Johannes
Published: July 2024

Solid particles as heat absorptances and storage mediums promise enhanced energy storage densities in concentrated solar power/thermal (CSP/T) plants. Employment of metallurgical slags as a secondary precursor material for solid particle preparation is ecologically and economically beneficial. Although these processed wastes, comprised of several oxides, exhibit generally promising high-temperature properties, chemical scattering from batch to batch may result in distinct material and functional properties, which may be an obstacle for their utilization. In this study, a steelmaking slag, LiDonit (LD), produced using a unique controlled slag treatment with high reproducibility is investigated as a candidate material. The aforementioned subsequent unique slag treatment makes LD a very promising and distinguishable secondary raw material for high-temperature applications. The as-received microstructure, phase components, and chemical composition of the LD material were analyzed to understand its material properties and to assess its reproducibility. The as-received LD chunks were transferred into pellets by subsequent milling, gel-casting, and sintering stages to reveal the potential processing routes. The CSP/T-related properties of sintered pellets, such as high temperature stability, heat capacity, and solar absorptance, were also examined to reveal their potential use in CSP/T applications and expand application areas with high added value.
Geochronology, Geochemistry, and In Situ Sr-Nd-Hf Isotopic Compositions of a Tourmaline-Bearing Leucogranite in Eastern Tethyan Himalaya: Implications for Tectonic Setting and Rare Metal Mineralization
Authors: Drolma Yangchen , Li Kaijun , Li Yubin , Zhang Jinshu , Yang Chengye , Zhang Gen , Li Ruoming , Liu Duo
Published: July 2024

Himalayan leucogranite is an excellent target for understanding the orogenic process of the India–Asia collision, but its origin and tectonic significance are still under debate. An integrated study of geochronology, geochemistry, and in situ Sr-Nd-Hf isotopes was conducted for a tourmaline-bearing leucogranite in the eastern Tethyan Himalaya using LA-ICP-MS, X-ray fluorescence spectroscopy, and ICP-MS and LA-MC-ICP-MS, respectively. LA-ICP-MS U-Pb dating of zircon and monazite showed that it was emplaced at ~19 Ma. The leucogranite had high SiO2 and Al2O3 contents ranging from 73.16 to 73.99 wt.% and 15.05 to 15.24 wt.%, respectively. It was characterized by a high aluminum saturation index (1.14–1.19) and Rb/Sr ratio (3.58–6.35), which is characteristic of S-type granite. The leucogranite was enriched in light rare-earth elements (LREEs; e.g., La and Ce) and large ion lithophile elements (LILEs; e.g., Rb, K, and Pb) and depleted in heavy rare-earth elements (e.g., Tm, Yb, and Lu) and high field strength elements (HFSEs; e.g., Nb, Zr, and Ti). It was characterized by high I Sr (t) (0.7268–0.7281) and low ε Nd (t) (−14.6 to −13.2) and ε Hf (t) (−12.6 to −9.47), which was consistent with the isotopic characteristics of the Higher Himalayan Sequence. Petrogenetically, the origin of the leucogranite is best explained by the decompression-induced muscovite dehydration melting of an ancient metapelitic source within the Higher Himalayan Sequence during regional extension due to the movement of the South Tibetan Detachment System (STDS). The significantly high lithium and beryllium contents of the leucogranite and associated pegmatite suggest that Himalayan leucogranites possess huge potential for lithium and beryllium exploration.
Methods of Pore Structural Characterisation of Sedimentary Rocks and Their Constituent Minerals
Authors: Rigby Sean P. , Himona Eleni
Published: July 2024

Pore structural characterisation is important for rocks and their constituent minerals in order to understand physico-chemical processes occurring therein. Rather than a broad general survey of potential pore characterisation techniques, this review focuses on an in-depth discussion of some key current issues in this topic. A so-called ‘brute-force’ characterisation approach involving a single imaging modality is seldom possible for rocks due to their high degree of heterogeneity. This work surveys alternate strategies suitable for rocks. Further, this work addresses some misapprehensions and misunderstandings that have arisen concerning some experimental techniques offering alternate strategies to the brute-force approach, such as gas overcondensation and mercury porosimetry. It also considers some pore structural characterisation techniques, such as cryoporometry, that are seldom used in the context of natural materials and surveys their capabilities.
Monitoring of Wall Thickness to Predict Corrosion in Marine Environments Using Ultrasonic Transducers
Authors: Salgueiro Francisca , Ribeiro Mário , Carvalho André , Covas Guilherme , Baltzersen Øystein , Proença Carla Sofia
Published: July 2024

The research related to subsea inspection, and the prediction of corrosion is a challenging task, and the progress in this area is continuously generating exciting new developments that may be used in subsea inspection. Wall thickness monitoring is an important tool to control and predict corrosion, such as on platforms for the infrastructure of floating offshore wind power production. This study shows the results obtained in marine environments. For this experiment, a steel plate equipped with ultrasound transducers was placed in seawater to corrode naturally. The sensor test setup consisted of 15 ultrasound transducers and 1 temperature sensor, which were installed in the cassette. The data acquisition system was based on a standard industrial computer with software written in Python and MATLAB. The ultrasound signals were collected at regular intervals and processed to calculate the instantaneous wall thickness. The progress of corrosion was evaluated by trend plots of wall thickness versus time, and the change in shape of the ultrasonic back wall reflection waveform measured by each sensor.
Carbohydrate Intake Levels and the Risk of Metabolic Syndrome in Korean Populations: A Prospective Study
Authors: Yoo Hyeonji , Jo Unhui , Park Kyong
Published: July 2024

In Korea and other Asian countries, traditional high-carbohydrate diets are increasingly associated with metabolic syndrome (MetS) and its complications. As dietary patterns shift, there is a growing need to assess the effect of these changes on health outcomes related to MetS. This study aimed to investigate the prospective relationship between carbohydrate consumption and the risk of MetS and its components. We analyzed data from 7902 participants from the Korean Association Resource, part of the Korean Genome and Epidemiology Study Dietary intakes, including carbohydrates and fiber, were assessed using a validated semi-quantitative food frequency questionnaire, allowing for the calculation of the proportion of total energy from carbohydrates (P_CARB) and the carbohydrate-to-fiber ratio to assess carbohydrate quality. Blood samples were collected after at least eight hours of fasting for laboratory analysis. We employed Cox proportional hazards models to estimate hazard ratios and 95% confidence intervals, focusing on the relationship between the P_CARB and the risk of developing MetS and its individual components, while adjusting for carbohydrate quality. In the fully adjusted model, which accounted for carbohydrate quality as a covariate, individuals in the highest percentile of the P_CARB showed a significantly increased risk of MetS, hypertriglyceridemia, hypo-high density lipoprotein cholesterolemia, dyslipidemia, and high blood pressure, compared to those in the lowest P_CARB group. Spline curve analyses indicated that the risks for MetS and its components consistently escalated with increasing P_CARB, with all p-values for nonlinearity exceeding 0.05. The findings suggest that higher levels of P_CARB are associated with an increased risk of MetS and related conditions, except for high fasting glucose. These results highlight the importance of dietary awareness and potential adjustments for populations consuming high-carbohydrate diets.
Usage of Chelating Gels in Root Canal Preparation: A Survey of Australian Clinicians
Authors: Wright Patricia P. , Diamond Elise S. , Peters Ove A.
Published: July 2024

Chelating gels may initially assist in root canal preparations to help establish a glide path. While irrigation surveys have investigated liquid chelators and gels to a minor extent, no endodontic survey has focused on EDTA gels and why clinicians use them. All 395 members of the Australian Society of Endodontology (ASE) were emailed an 11-question survey concerning chelation gel usage via Qualtrics. Paper copies were also distributed at ASE events. Descriptive statistics were performed for practitioner and chelating gel data. Cross-tabulations of the practitioner type with the case percentage usage of gels were analyzed with the chi square test (adjusted p value < 0.0083). The overall response rate was 181/395 (46%). For 174 eligible responses, those who never/rarely used a gel were as follows: endodontic residents 12/14 (86%), endodontists 53/71 (75%), general dentists 39/86 (45%), and other members 0% (0/3). Most gel users said that gels helped establish a glide path. Those who never/rarely used chelating gels commented that they held no benefits over liquid EDTA. Endodontists were less likely to use a chelation gel than general dentists (p < 0.0083). Older clinicians had higher gel usage than younger ASE members (p < 0.0083), indicating a possible trend to diminished gel usage over time amongst ASE members. In conclusion, most ASE members find no need for a chelating gel, raising questions about their clinical effectiveness. Studies would therefore be warranted to investigate gel efficacy and improved clinical outcomes.
Progress on the Regulation of the Host Immune Response by Parasite-Derived Exosomes
Authors: Zhang Xinyue , Yu Chuanxin , Song Lijun
Published: July 2024

Exosomes are membrane-bound structures released by cells into the external environment that carry a significant amount of important cargo, such as proteins, DNA, RNA, and lipids. They play a crucial role in intercellular communication. Parasites have complex life cycles and can release exosomes at different stages. Exosomes released by parasitic pathogens or infected cells contain parasitic nucleic acids, antigenic molecules, virulence factors, drug-resistant proteins, proteases, lipids, etc. These components can regulate host gene expression across species or modulate signaling pathways, thereby dampening or activating host immune responses, causing pathological damage, and participating in disease progression. This review focuses on the means by which parasitic exosomes modulate host immune responses, elaborates on the pathogenic mechanisms of parasites, clarifies the interactions between parasites and hosts, and provides a theoretical basis and research directions for the prevention and treatment of parasitic diseases.
In Vitro Antiviral Activity of Rhodiola crenulata Extract against Zika Virus and Japanese Encephalitis Virus: Viral Binding and Stability
Authors: Lai Zheng-Zong , Yen I-Chuan , Hung Hao-Yuan , Hong Chen-Yang , Lai Chih-Wei , Lee Yen-Mei
Published: July 2024

Zika virus (ZIKV) and Japanese encephalitis virus (JEV) can cause permanent neurological damage and death, yet no approved drugs exist for these infections. Rhodiola crenulate, an herb used in traditional Chinese medicine for its antioxidation and antifatigue properties, was studied for its antiviral activity against ZIKV and JEV in vitro. The cytotoxicity of Rhodiola crenulata extract (RCE) was evaluated using the CCK-8 reagent. Antiviral effects of RCE were assessed in ZIKV-infected or JEV-infected Vero cells via quantitative reverse transcription polymerase chain reaction (qRT-PCR), Western blotting, fluorescent focus assay (FFA), and immunofluorescence assay (IFA). The cell-free antiviral effects of RCE were evaluated using an inactivation assay. To determine the stage of the viral life cycle affected by RCE, time-of-addition, binding, and entry assays were conducted. Three bioactive constituents of RCE (salidroside, tyrosol, and gallic acid) were tested for antiviral activity. RCE exhibited dose-dependent anti-ZIKV and anti-JEV activities at non-cytotoxic concentrations, which were likely achieved by disrupting viral binding and stability. Gallic acid exhibited antiviral activity against ZIKV and JEV. Our findings indicate that RCE disrupts viral binding and stability, presenting a potential strategy to treat ZIKV and JEV infections.
Advances and Challenges in Immune-Modulatory Biomaterials for Wound Healing Applications
Authors: Cao Yuqi , Sun Jiagui , Qin Shengao , Zhou Zhengshu , Xu Yanan , Liu Chenggang
Published: July 2024

Wound healing progresses through three distinct stages: inflammation, proliferation, and remodeling. Immune regulation is a central component throughout, crucial for orchestrating inflammatory responses, facilitating tissue repair, and restraining scar tissue formation. Elements such as mitochondria, reactive oxygen species (ROS), macrophages, autophagy, ferroptosis, and cytokines collaboratively shape immune regulation in this healing process. Skin wound dressings, recognized for their ability to augment biomaterials’ immunomodulatory characteristics via antimicrobial, antioxidative, pro- or anti-inflammatory, and tissue-regenerative capacities, have garnered heightened attention. Notwithstanding, a lack of comprehensive research addressing how these dressings attain immunomodulatory properties and the mechanisms thereof persists. Hence, this paper pioneers a systematic review of biomaterials, emphasizing immune regulation and their underlying immunological mechanisms. It begins by highlighting the importance of immune regulation in wound healing and the peculiarities and obstacles faced in skin injury recovery. This segment explores the impact of wound metabolism, infections, systemic illnesses, and local immobilization on the immune response during healing. Subsequently, the review examines a spectrum of biomaterials utilized in skin wound therapy, including hydrogels, aerogels, electrospun nanofiber membranes, collagen scaffolds, microneedles, sponges, and 3D-printed constructs. It elaborates on the immunomodulatory approaches employed by these materials, focusing on mitochondrial and ROS modulation, autophagic processes, ferroptosis, macrophage modulation, and the influence of cytokines on wound healing. Acknowledging the challenge of antibiotic resistance, the paper also summarizes promising plant-based alternatives for biomaterial integration, including curcumin. In its concluding sections, the review charts recent advancements and prospects in biomaterials that accelerate skin wound healing via immune modulation. This includes exploring mitochondrial transplantation materials, biomaterial morphology optimization, metal ion incorporation, electrostimulation-enabled immune response control, and the benefits of composite materials in immune-regulatory wound dressings. The ultimate objective is to establish a theoretical foundation and guide future investigations in the realm of skin wound healing and related materials science disciplines.
Phytochemical Composition and Biological Activity of the Essential Oil from Ericameria nauseosa Collected in Southwestern Montana, United States
Authors: Schepetkin Igor A. , Özek Gulmira , Özek Temel , Kirpotina Liliya N. , Khlebnikov Andrei I. , Ayçiçek Kevser , Lavin Matthew , Quinn Mark T.
Published: July 2024

Ericameria nauseosa (Pall. ex Pursh) G.L. Nesom & G.I. Baird) is used in traditional medicine to treat various diseases; however, little is known about the immunomodulatory activity of essential oil from this plant. Thus, we isolated essential oil from the aerial parts of E. nauseosa and evaluated their chemical composition and biological activity. Compositional analysis of E. nauseosa essential oil revealed that the main (>2%) components were γ-decalactone (13.3%), cryptone (9.4%), terpinen-4-ol (9.3%), (E)-methyl cinnamate (6.0%), T-cadinol (4.7%), spathulenol (3.6%), 8Z-2,3-dihydromatricaria ester (3.1%), β-phellandrene (3.0%), p-cymen-8-ol (2.2%), 3-ethoxy-2-cycloocten-1-one (2.2%), and trans-p-menth-2-en-1-ol (2.1%). Distinctive features were the lactones (up to 15%) and polyacetylenes (up to 3.1%), including (2Z,8Z)-matricaria ester and 8Z-2,3-dihydromatricaria ester. A comparison with other reported E. nauseosa essential oil samples showed that our samples were distinct from those collected in other areas of the country; however, they did have the most similarity to one sample collected in North Central Utah. Pharmacological studies showed that E. nauseosa essential oil activated human neutrophil Ca2+ influx, which desensitized these cells to subsequent agonist-induced functional responses. Based on our previously reported data that nerolidol, β-pinene, spathulenol, sabinene, and γ-terpinene were active in human neutrophils, these compounds are the most likely constituents contributing to this immunomodulatory activity. However, the relatively high amount of polyacetylenes may also contribute, as these compounds have been characterized as potent immunomodulators.
Extraction of Bioactive Compounds from the Fruits of Jambolan (Syzygium cumini (L.)) Using Alternative Solvents
Authors: Artilha-Mesquita Carla Adriana Ferrari , Stafussa Ana Paula , Santos Patrícia Daniele Silva dos , Santos Oscar de Oliveira , da Costa Silvio Claudio , Madrona Grasiele Scaramal
Published: July 2024

This work demonstrates the effectiveness of using alternative solvents to obtain jambolan extracts with a high content of bioactive compounds compared to conventional organic solvents, being the first study to evaluate the best ecological solvent alternative for Syzygium cumini (L.) Skeels. Five alternative solvents were used for extraction: water at 25 °C (W25), water at 50 °C (W50), water at 75 °C (W75), water with citric acid at 2.4% (CA2), and water with citric acid at 9.6% (CA9) in comparison with three conventional solvents: ethanol (EtOH), water with ethanol at 50% (WE), and water with methanol at 50% (WM). A protocol was then established for the extraction and concentration of samples obtained with these solvents. The highest content of total phenolic compounds (TPCs) in the extracts was obtained with the solvent W75 (1347.27 mg GAE/100 g), while in the concentrates it was the solvents EtOH (3823.03 mg GAE/100 g) and WM (4019.39 mg GAE/100 g). Total monomeric anthocyanins (TMAs) increased by 209.31% and 179.95% in extractions with CA2 and CA9, respectively, compared to pulp (35.57 mg eq c-3-g/100 g), demonstrating that they are the most efficient alternative solvents in this extraction. The levels of bioactive compounds and antioxidant activity varied according to the solvents used. Delphinidin 3,5-diglucoside, cyanidin 3,5-diglucoside, delphinidin 3-glucoside, petunidin 3,5-diglucoside, cyanidin 3-glucoside, peonidin 3,5-diglucoside, malvidin 3,5-diglucoside, petunidin 3-glucoside, and malvidin 3-glucoside were identified in most of the samples by UPLC-MS/MS. This study suggests that a simple procedure using alternative solvents can be used as an environmentally friendly strategy to achieve efficient extraction of bioactive compounds in jambolan.
Study of CaDreb2c and CaDreb2h Gene Sequences and Expression in Chickpea (Cicer arietinum L.) Cultivars Growing in Northern Kazakhstan under Drought
Authors: Kiselev Konstantin V. , Ogneva Zlata V. , Dubrovina Alexandra S. , Gabdola Ademi Zh. , Khassanova Gulmira Zh. , Jatayev Satyvaldy A.
Published: July 2024

Drought poses a significant challenge to plant growth and productivity, particularly in arid regions like northern Kazakhstan. Dehydration-responsive element-binding (DREB) transcription factors play an important role in plant response to drought and other abiotic stresses. In Arabidopsis thaliana, the DREB subfamily consists of six groups, designated DREB1 to DREB6. Among these, DREB2 is primarily associated with drought and salinity tolerance. In the chickpea genome, two DREB genes, CaDREB2c and CaDREB2h, have been identified, exhibiting high sequence similarity to Arabidopsis DREB2 genes. We investigated the nucleotide sequences of CaDREB2c and CaDREB2h genes in several chickpea cultivars commonly grown in northern Kazakhstan. Interestingly, the CaDREB2h gene sequence was identical across all varieties and corresponded to the sequence deposited in the GenBank. However, the CaDREB2c gene sequence exhibited variations among the studied varieties, categorized into three groups: the first group (I), comprising 20 cultivars, contained a CaDREB2c gene sequence identical to the GenBank (Indian cultivar CDC Frontier). The second group (II), consisting of 4 cultivars, had a single synonymous substitution (T to C) compared to the deposited CaDREB2c gene sequence. The third group, encompassing 5 cultivars, displayed one synonymous substitution (C to T) and two non-synonymous substitutions (G to T and G to A). Furthermore, we assessed the gene expression patterns of CaDREB2c and CaDREB2h in different chickpea varieties under drought conditions. Chickpea cultivars 8 (III), 37 (I), 6 (III), and 43 (I) exhibited the highest drought resistance. Our analysis revealed a strong positive correlation between drought resistance and CaDREB2h gene expression under drought stress. Our findings suggest that the chickpea’s adaptive responses to water deprivation are associated with changes in CaDREB2 gene expression. To further elucidate the mechanisms underlying drought tolerance, we propose future research directions that will delve into the molecular interactions and downstream targets of CaDREB2 genes.
Genome-Wide Analysis of DNA Demethylases in Land Plants and Their Expression Pattern in Rice
Authors: Mao Shengxin , Xiao Jian , Zhao Yating , Hou Jiaqi , Li Lijia
Published: July 2024

DNA demethylation is a very important biochemical pathway regulating a group of biological processes, such as embryo development, fruit ripening, and response to stress. Despite the essential role of DNA demethylases, their evolutionary relationship and detailed biological functions in different land plants remain unclear. In this study, 48 DNA demethylases in 12 land plants were identified and classified. A phylogenetic tree was constructed to demonstrate the evolutionary relationships among these DNA demethylases, indicating how they are related across different species. Conserved domain, protein motif, and gene structure analysis showed that these 48 DNA demethylases fell into the presently identified four classes of DNA demethylases. Amino acid alignment revealed conserved catalytic sites and a previously less-studied protein region (referred to as domain A) within the DNA demethylases. An analysis showed a conserved pattern of gene duplication for DNA demethylases throughout their evolutionary history, suggesting that these genes had been maintained due to their importance. The examination of promoter cis-elements displayed potential signaling and regulating pathways of DNA demethylases. Furthermore, the expression profile was analyzed to investigate the physiological role of rice DNA demethylase in different developmental stages, in tissues, and in response to stress and various phytohormone signals. The findings offer a deeper insight into the functional regions of DNA demethylases and their evolutionary relationships, which can guide future research directions. Understanding the role of DNA demethylases can lead to improved plant stress resistance and contribute to the development of better crop and fruit varieties.
Genome-Wide Identification and Expression Characterization of the D27 Gene Family of Capsicum annuum L.
Authors: Wu Di , Fu Wenting , Wang Nanyi , Ye Yong , He Jianwen , Wu Kangyun
Published: July 2024

As a crucial member of the gene family involved in the biosynthesis of strigolactones, D27 plays an important regulatory role in plant branching and root development, which is essential for field management and yield increase in peppers (Capsicum annuum L.). To comprehensively understand the characteristics of the pepper D27 gene family, we identified three CaD27 genes. By analyzing their physicochemical properties, phylogenetic relationships, gene structures, promoters, and expression patterns in different tissues, the characteristics of the CaD27 gene family were revealed. The research results showed that these three CaD27 genes are located in three different chromosomes. Evolutionary analysis divided the members of CaD27 into three groups, and gene collinearity analysis did not find any duplicates, indicating the diversity and non-redundancy of the CaD27 gene family members. In addition, we identified and classified cis-elements in the promoter regions of CaD27 genes, with a relatively high proportion related to light and plant hormone responses. Expression pattern analysis showed that CaD27.1 is expressed in leaves, while CaD27.2 is expressed in roots, indicating tissue specificity. Furthermore, protein interaction predictions revealed an interaction between D27.2 and CCD7. This study provided important insights into the function and regulatory mechanisms of the CaD27 gene family and the role of strigolactones in plant growth and development.
Effect of Bi2O3 Particle Size on the Radiation-Shielding Performance of Free-Lead Epoxide Materials against Ionizing Radiation
Authors: Hedaya Ali , Elsafi Mohamed , Al-Saleh Wafa M. , Saleh Ibrahim H.
Published: July 2024

In this work, we studied the effect of bismuth oxide particle size and its attenuation capacity as a filler additive in epoxy resins. Six samples were prepared according to the amount of microparticles and nanoparticles in the sample and were coded as ERB-1, ERB-2, ERB-3, ERB-4, ERB-5, and ERB-6. One of the composite epoxies contained Bi2O3 microparticles at a 50:50 ratio (ERB-6) and was chosen as the control composite, and the number of microparticles (MPs) was gradually decreased and replaced by nanoparticles (NPs) to produce epoxy-containing Bi2O3 nanoparticles at a 50:50 ratio (ERB-1). The morphological and thermal characteristics of the studied composites were tested. The attenuation capability of the prepared composites, which is determined by the Bi2O3 particle size, was determined experimentally using a semiconductor detector, an HPGe-detector, and three different gamma-ray point sources (Am-241, Co-60, and Cs-137). The linear attenuation coefficient (LAC) of ERB-3, which contained 30% nanoparticles and 20% microparticles, had the highest value compared to the other composites at all the energies discussed, while the ERB-6 composite had the lowest value at all energies. The radiation-shielding efficiency (RSE) of the prepared samples was determined at all discussed energies; at 662 keV, the radiation-shielding efficiency values were 15.97%, 13.94%, and 12.55% for ERB-3, ERB-1, and ERB-6, respectively. The statistics also proved that the attenuation capacities of the samples containing a combination of nanoparticles and microparticles were much superior to those of the samples containing only microparticles or nanoparticles. A ranking of the samples based on their attenuation capacity is as follows: ERB-3 > ERB-4 > ERB-2 > ERB-1 > ERB-5 > ERB-6.
Isoreticular Metal-Organic Framework-3 (IRMOF-3): From Experimental Preparation, Functionalized Modification to Practical Applications
Authors: Ning Haoyue , Lu Lixin
Published: July 2024

Isoreticular metal-organic framework-3 (IRMOF-3), a porous coordination polymer, is an MOF material with the characteristics of a large specific surface area and adjustable pore size. Due to the existence of the active amino group (-NH2) on the organic ligand, IRMOF-3 has more extensive research and application potential. Herein, the main preparation methods of IRMOF-3 in existing research were compared and discussed first. Second, we classified and summarized the functionalization modification of IRMOF-3 based on different reaction mechanisms. In addition, the expanded research and progress of IRMOF-3 and their derivatives in catalysis, hydrogen storage, material adsorption and separation, carrier materials, and fluorescence detection were discussed from an application perspective. Moreover, the industrialization prospect of IRMOF-3 and the pressing problems in its practical application were analyzed and prospected. This review is expected to provide a reference for the design and application of more new nanomaterials based on IRMOF-3 to develop more advanced functional materials in industrial production and engineering applications.