bluuebunny/crossref_metadata_2025 huggingface dataset:
Fine-Scale Ocean Currents Derived From in situ Observations in Anticipation of the Upcoming SWOT Altimetric Mission
Authors: Barceló-Llull Bàrbara , Pascual Ananda , Sánchez-Román Antonio , Cutolo Eugenio , d'Ovidio Francesco , Fifani Gina , Ser-Giacomi Enrico , Ruiz Simón , Mason Evan , Cyr Fréderic , Doglioli Andrea , Mourre Baptiste , Allen John T. , Alou-Font Eva , Casas Benjamín , Díaz-Barroso Lara , Dumas Franck , Gómez-Navarro Laura , Muñoz Cristian
Published: August 2021

After the launch of the Surface Water and Ocean Topography (SWOT) satellite planned for 2022, the region around the Balearic Islands (western Mediterranean Sea) will be the target of several in situ sampling campaigns aimed at validating the first available tranche of SWOT data. In preparation for this validation, the PRE-SWOT cruise in 2018 was conceived to explore the three-dimensional (3D) circulation at scales of 20 km that SWOT aims to resolve, included in the fine-scale range (1–100 km) as defined by the altimetric community. These scales and associated variability are not captured by contemporary nadir altimeters. Temperature and salinity observations reveal a front that separates local Atlantic Water in the northeast from recent Atlantic Water in the southeast, and extends from the surface to ~150 m depth with maximum geostrophic velocities of the order of 0.20 m s−1 and a geostrophic Rossby number that ranges between −0.24 and 0.32. This front is associated with a 3D vertical velocity field characterized by an upwelling cell surrounded by two downwelling cells, one to the east and the other to the west. The upwelling cell is located near an area with high nitrate concentrations, possibly indicating a recent inflow of nutrients. Meanwhile, subduction of chlorophyll-a in the western downwelling cell is detected in glider observations. The comparison of the altimetric geostrophic velocity with the CTD-derived geostrophic velocity, the ADCP horizontal velocity, and drifter trajectories, shows that the present-day resolution of altimetric products precludes the representation of the currents that drive the drifter displacement. The Lagrangian analysis based on these velocities demonstrates that the study region has frontogenetic dynamics not detected by altimetry. Our results suggest that the horizontal component of the flow is mainly geostrophic down to scales of 20 km in the study region and during the period analyzed, and should therefore be resolvable by SWOT and other future satellite-borne altimeters with higher resolutions. In addition, fine-scale features have an impact on the physical and biochemical spatial variability, and multi-platform in situ sampling with a resolution similar to that expected from SWOT can capture this variability.
Temperature-Driven Growth Variation in a Deep-Sea Fish: The Case of Pagellus bogaraveo (Brünnich, 1768) in the Azores Archipelago
Authors: Neves João , Giacomello Eva , Menezes Gui M. , Fontes Jorge , Tanner Susanne E.
Published: August 2021

The deep ocean ecosystem hosts high biodiversity and plays a critical role for humans through the ecosystem services it provides, such as fisheries and climate regulation. However, high longevity, late reproduction, and low fecundity of many organisms living in the deep ocean make them particularly vulnerable to fishing and climate change. A better understanding of how exploitation and changing environmental conditions affect life-history parameters (e.g., growth) of commercially important fish species is crucial for their long-term sustainable management. To this end, we used otolith increment widths and a mixed-effects modeling approach to develop a 42-year growth chronology (1975–2016) of the commercially important deep-sea fish species blackspot seabream (Pagellus bogaraveo) among the three island groups of the Azores archipelago (Northeast Atlantic). Growth was related to intrinsic (age and age-at-capture) and extrinsic factors (capture location, temperature-at-depth, North Atlantic Oscillation (NAO), Eastern Atlantic Pattern (EAP), and proxy for exploitation (landings)). Over the four decades analyzed, annual growth patterns varied among the three island groups. Overall, temperature-at-depth was the best predictor of growth, with warmer water associated with slower growth, likely reflecting physiological conditions and food availability. Average population growth response to temperature was separated into among-individual variation and within-individual variation. The significant among-individual growth response to temperature was likely related to different individual-specific past experiences. Our results suggested that rising ocean temperature may have important repercussions on growth, and consequently on blackspot seabream fishery production. Identifying drivers of blackspot seabream growth variation can improve our understanding of past and present condition of the populations toward the sustainable management of the fishery.
COVID-19 Pneumonia: The Great Ultrasonography Mimicker
Authors: Lacedonia Donato , Quarato Carla Maria Irene , Mirijello Antonio , Trovato Guglielmo M. , Del Colle Anna , Rea Gaetano , Scioscia Giulia , Foschino Barbaro Maria Pia , Sperandeo Marco
Published: August 2021

The pandemic spread of the new severe acute respiratory syndrome coronavirus 2 has raised the necessity to identify an appropriate imaging method for early diagnosis of coronavirus disease 2019 (COVID-19). Chest computed tomography (CT) has been regarded as the mainstay of imaging evaluation for pulmonary involvement in the early phase of the pandemic. However, due to the poor specificity of the radiological pattern and the disruption of radiology centers' functionality linked to an excessive demand for exams, the American College of Radiology has advised against CT use for screening purposes. Lung ultrasound (LUS) is a point-of-care imaging tool that is quickly available and easy to disinfect. These advantages have determined a “pandemic” increase of its use for early detection of COVID-19 pneumonia in emergency departments. However, LUS findings in COVID-19 patients are even less specific than those detectable on CT scans. The scope of this perspective article is to discuss the great number of diseases and pathologic conditions that may mimic COVID-19 pneumonia on LUS examination.
ARDS Patients Exhibiting a “Hyperinflammatory Anasarca” Phenotype Could Benefit From a Conservative Fluid Management Strategy
Authors: Xing Chun-yan , Gong Wen-bin , Yang Yan-Na , Qi Xin-jie , Zhang Shi
Published: August 2021

Object: The fluid management strategy in ARDS is not very clear. A secondary analysis of RCT data was conducted to identify patients with ARDS benefitting from a conservative strategy of fluid management.

Methods: The data of this study were downloaded from the ARDS network series of randomized controlled trials (Conservative Strategy vs. Liberal Strategy in 2006). Based on the clinical feature of patients, within the first 24 h after admission, clustering was performed using the k-means clustering algorithm to identify the phenotypes of ARDS. Survival was analyzed using the Kaplan-Meier survival analysis to assess the effect of the two fluid management strategies on the 90-day cumulative mortality. Categorical/dichotomic variables were analyzed by the chi-square test. Continuous variables were expressed as the mean and standard deviation and evaluated through a one-way ANOVA. A P-value < 0.05 was defined as the statistically significant cut-off value.

Results: A total of 1,000 ARDS patients were enrolled in this unsupervised clustering research study, of which 503 patients were treated with a conservative fluid-management strategy, and 497 patients were treated with a liberal fluid-management strategy. The first 7-day cumulative fluid balance in patients with the conservative strategy and liberal strategy were −136 ± 491 ml and 6,992 ± 502 ml, respectively (P < 0.001). Four phenotypes were found, and the conservative fluid-management strategy significantly improved the 90-day cumulative mortality compared with the liberal fluid-management strategy (HR = 0.532, P = 0.024) in patients classified as “hyperinflammatory anasarca” phenotype (phenotype II). The characteristics of this phenotype exhibited a higher WBC count (20487.51 ± 7223.86/mm3) with a higher incidence of anasarca (8.3%) and incidence of shock (26.6%) at baseline. The furthermore analysis found that the conservative fluid management strategy was superior to the liberal fluid management strategy in avoiding superinfection (10.10 vs. 14.40%, P = 0.037) and returned to assisted breathing (4.60 vs. 16.20%, P = 0.030) in patients classified as “hyperinflammatory anasarca” phenotype. In addition, patients with other phenotypes given the different fluid management strategies did not show significant differences in clinical outcomes.

Conclusion: Patients exhibiting a “hyperinflammatory anasarca” phenotype could benefit from a conservative fluid management strategy.
Major Insights in Dynamics of Host Response to SARS-CoV-2: Impacts and Challenges
Authors: Hakim Al , Hasan Md. Mahbub , Hasan Mahmudul , Lokman Syed Mohammad , Azim Kazi Faizul , Raihan Topu , Chowdhury Parveen Afroz , Azad Abul Kalam
Published: August 2021

The coronavirus disease 2019 (COVID-19), a pandemic declared by the World Health Organization on March 11, 2020, is caused by the infection of highly transmissible species of a novel coronavirus called severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). As of July 25, 2021, there are 194,372,584 cases and 4,167,937 deaths with high variability in clinical manifestations, disease burden, and post-disease complications among different people around the globe. Overall, COVID-19 is manifested as mild to moderate in almost 90% of the cases and only the rest 10% of the cases need hospitalization. However, patients with older age and those having different comorbidities have made worst the pandemic scenario. The variability of pathological consequences and clinical manifestations of COVID-19 is associated with differential host–SARS-CoV-2 interactions, which are influenced by the factors that originated from the SARS-CoV-2 and the host. These factors usually include the genomic attributes and virulent factors of the SARS-CoV-2, the burden of coinfection with other viruses and bacteria, age and gender of the individuals, different comorbidities, immune suppressions/deficiency, genotypes of major histocompatibility complex, and blood group antigens and antibodies. We herein retrieved and reviewed literatures from PubMed, Scopus, and Google relevant to clinical complications and pathogenesis of COVID-19 among people of different age, sex, and geographical locations; genomic characteristics of SARS-CoV-2 including its variants, host response under different variables, and comorbidities to summarize the dynamics of the host response to SARS-CoV-2 infection; and host response toward approved vaccines and treatment strategies against COVID-19. After reviewing a large number of published articles covering different aspects of host response to SARS-CoV-2, it is clear that one aspect from one region is not working with the scenario same to others, as studies have been done separately with a very small number of cases from a particular area/region of a country. Importantly, to combat such a pandemic as COVID-19, a conclusive understanding of the disease dynamics is required. This review emphasizes on the identification of the factors influencing the dynamics of host responses to SARS-CoV-2 and offers a future perspective to explore the molecular insights of COVID-19.
Monitoring Urban Beach Quality on a Summer Day: Determination of the Origin of Fecal Indicator Bacteria and Antimicrobial Resistance at Prophète Beach, Marseille (France)
Authors: Toubiana Mylène , Salles Christian , Tournoud Marie-George , Licznar-Fajardo Patricia , Zorgniotti Isabelle , Trémélo Marie-Laure , Jumas-Bilak Estelle , Robert Samuel , Monfort Patrick
Published: August 2021

A highly frequented beach in Marseille, France, was monitored on an hourly basis during a summer day in July 2018, to determine possible water and sand fecal pollution, in parallel with influx of beach users from 8 a.m. to 8 p.m. Fecal indicator bacteria were enumerated, together with four host-associated fecal molecular markers selected to discriminate human, dog, horse, or gull/seagull origins of the contamination. The antimicrobial resistance of bacteria in water and sand was evaluated by quantifying (i) the class 1, 2, and 3 integron integrase genes intI, and (ii) blaTEM, blaCTX–M, and blaSHV genes encoding endemic beta-lactamase enzymes. The number of beach users entering and leaving per hour during the observation period was manually counted. Photographs of the beach and the bathing area were taken every hour and used to count the number of persons in the water and on the sand, using a photo-interpretation method. The number of beach users increased from early morning to a peak by mid-afternoon, totaling more than 1,800, a very large number of users for such a small beach (less than 1 ha). An increase in fecal contamination in the water corresponded to the increase in beach attendance and number of bathers, with maximum numbers observed in the mid-afternoon. The human-specific fecal molecular marker HF183 indicated the contamination was of human origin. In the water, the load of Intl2 and 3 genes was lower than Intl1 but these genes were detected only during peak attendance and highest fecal contamination. The dynamics of the genes encoding B-lactamases involved in B-lactams resistance notably was linked to beach attendance and human fecal contamination. Fecal indicator bacteria, integron integrase genes intI, and genes encoding B-lactamases were detected in the sand. This study shows that bathers and beach users can be significant contributors to contamination of seawater and beach sand with bacteria of fecal origin and with bacteria carrying integron-integrase genes and beta lactamase encoding genes. High influx of users to beaches is a significant factor to be considered in order to reduce contamination and manage public health risk.
Response of Soil Microbial Community to Vegetation Reconstruction Modes in Mining Areas of the Loess Plateau, China
Authors: Zhao Jiao , Ma Jing , Yang Yongjun , Yu Haochen , Zhang Shaoliang , Chen Fu
Published: August 2021

Vegetation reconstruction and restoration is vital to the health of the mine land ecosystem. Different vegetations might change microbial community structure and function of soil, mediating the biogeochemical cycle and nutrition supply to the soil. To clarify the response of soil microbes to different vegetation reconstruction modes in the mining areas of the Loess Plateau, China, soil microbial community structures and functions were determined by the MiSeq high-throughput sequencing along with PICRUSt2 and FUNGuild tools. The fungal community richness was observed to be the highest in grassland soil and positively correlated with soil organic matter, total nitrogen, and nitrate-nitrogen. The bacterial and fungal community structures were similar in grassland and brushland areas, but were significantly differentiated in the coniferous and broadleaf forest, and the leading factors were soil pH and nitrate-nitrogen. Actinobacteriota, Proteobacteria, and Acidobacteriota were the dominant bacterial phyla under different vegetation reconstruction modes. The dominant phyla of fungi were Ascomycota, Basidiomycota, and Mortierellomycota. Different vegetation reconstruction modes did not affect the bacterial functional communities but shaped different functional groups of fungi. The grassland soil was dominated by saprotrophic fungi, while symbiotrophic fungi dominated the coniferous and broadleaf forests. The results suggested that shifts in vegetation reconstruction modes may alter the mining soil bacterial and fungal community structures and function. These findings improve the understanding of microbial ecology in the reclaimed mine soil and provide a reference for the ecological restoration of fragile mining ecosystems.
Extracellular and Intracellular Angiotensin II Regulate the Automaticity of Developing Cardiomyocytes via Different Signaling Pathways
Authors: Qi Zenghua , Wang Tao , Chen Xiangmao , Wong Chun Kit , Ding Qianqian , Sauer Heinrich , Chen Zhi-Feng , Long Cheng , Yao Xiaoqiang , Cai Zongwei , Tsang Suk Ying
Published: August 2021

Angiotensin II (Ang II) plays an important role in regulating various physiological processes. However, little is known about the existence of intracellular Ang II (iAng II), whether iAng II would regulate the automaticity of early differentiating cardiomyocytes, and the underlying mechanism involved. Here, iAng II was detected by immunocytochemistry and ultra-high performance liquid chromatography combined with electrospray ionization triple quadrupole tandem mass spectrometry in mouse embryonic stem cell–derived cardiomyocytes (mESC-CMs) and neonatal rat ventricular myocytes. Expression of AT1R-YFP in mESC-CMs revealed that Ang II type 1 receptors were located on the surface membrane, while immunostaining of Ang II type 2 receptors (AT2R) revealed that AT2R were predominately located on the nucleus and the sarcoplasmic reticulum. While extracellular Ang II increased spontaneous action potentials (APs), dual patch clamping revealed that intracellular delivery of Ang II or AT2R activator C21 decreased spontaneous APs. Interestingly, iAng II was found to decrease the caffeine-induced increase in spontaneous APs and caffeine-induced calcium release, suggesting that iAng II decreased spontaneous APs via the AT2R- and ryanodine receptor–mediated pathways. This is the first study that provides evidence of the presence and function of iAng II in regulating the automaticity behavior of ESC-CMs and may therefore shed light on the role of iAng II in fate determination.
The Specific NLRP3 Antagonist IFM-514 Decreases Fibrosis and Inflammation in Experimental Murine Non-Alcoholic Steatohepatitis
Authors: Torres Sandra , Brol Maximilian J , Magdaleno Fernando , Schierwagen Robert , Uschner Frank E. , Klein Sabine , Ortiz Cristina , Tyc Olaf , Bachtler Nadine , Stunden James , Bertheloot Damien , Kitanovic Ana , Sanchez Brian , Schrum Jacob , Roush William R. , Franchi Luigi , Byth Kate , Latz Eicke , Trebicka Jonel
Published: August 2021

Background and Aims: Activation of the inflammasome NLRP3 (NOD-, LRR- and pyrin domain containing 3) contributes to the development of non-alcoholic fatty liver disease (NAFLD) and progression to non-alcoholic steatohepatitis (NASH). Therefore, this study explored the therapeutic effects of a novel and selective NLRP3 antagonist in a murine dietary model of NASH.

Methods: Groups of 12-week-old ApoE-/- mice were fed ad lib for 7 weeks with a methionine/choline deficient (MCD) and western diet (WD). After 3 weeks of diet-induced injury, mice were injected i. p. with the NLRP3 antagonist IFM-514 (100 mg/kg body weight) or vehicle (0.5% carmellose) every day, 5 days/week for a further 4 weeks. Several markers of inflammation, fibrosis and steatosis were evaluated. Whole transcriptome sequencing and panel RNA expression analysis (NanoString) were performed.

Results: IFM-514 inhibited IL-1β production in mice challenged with 20 mg/kg lipopolysaccharide, and in mouse and human inflammatory cells in vitro. IFM-514 inhibited hepatic inflammation in the in vivo non-alcoholic steatohepatitis model assessed by H&E staining and in the hepatic gene expression of inflammasome-related proinflammatory cytokines. This effect was associated with significant reduction in caspase-1 activation. Similarly, IFM-514 was efficacious in vivo in MDC-fed ApoE-/- mice, markedly reducing portal pressure, Sirius red staining and 4-hydroxyproline content compared to vehicle-treated mice. Moreover, IFM-514 significantly reduced hepatic steatosis in MCD-fed ApoE-/- mice, as evidenced by NAFLD scores, oil red O staining, hepatic triglycerides and gene expression. In WD treated animals, similar trends in inflammation and fibrosis were observed, although not sufficient IFM-514 levels were reached.

Conclusion: Overall, IFM-514 reduced liver inflammation and fibrosis, with mild effects on liver steatosis in experimental murine NASH. Blocking of NLRP3 may be an attractive therapeutic approach for NASH patients.
Structural Polymorphism of Chitin and Chitosan in Fungal Cell Walls From Solid-State NMR and Principal Component Analysis
Authors: Fernando Liyanage D. , Dickwella Widanage Malitha C. , Penfield Jackson , Lipton Andrew S. , Washton Nancy , Latgé Jean-Paul , Wang Ping , Zhang Liqun , Wang Tuo
Published: August 2021

Chitin is a major carbohydrate component of the fungal cell wall and a promising target for novel antifungal agents. However, it is technically challenging to characterize the structure of this polymer in native cell walls. Here, we recorded and compared 13C chemical shifts of chitin using isotopically enriched cells of six Aspergillus, Rhizopus, and Candida strains, with data interpretation assisted by principal component analysis (PCA) and linear discriminant analysis (LDA) methods. The structure of chitin is found to be intrinsically heterogeneous, with peak multiplicity detected in each sample and distinct fingerprints observed across fungal species. Fungal chitin exhibits partial similarity to the model structures of α- and γ-allomorphs; therefore, chitin structure is not significantly affected by interactions with other cell wall components. Addition of antifungal drugs and salts did not significantly perturb the chemical shifts, revealing the structural resistance of chitin to external stress. In addition, the structure of the deacetylated form, chitosan, was found to resemble a relaxed two-fold helix conformation. This study provides high-resolution information on the structure of chitin and chitosan in their cellular contexts. The method is applicable to the analysis of other complex carbohydrates and polymer composites.
Prognostic Value of a New Integrated Parameter—Both Collateral Circulation and Permeability Surface—in Hemorrhagic Transformation of Middle Cerebral Artery Occlusion Acute Ischemic Stroke: Retrospective Cohort Study
Authors: Li Chanchan , Hao Xiaozhu , Lin Luyi , Sun Chengfeng , Yu Hai , Yao Zhenwei , Feng Xiaoyuan , Yang Yanmei
Published: August 2021

Background

Multimodal CT, including CT angiography (CTA) and CT perfusion (CTP), was increasingly used in stroke triage. This study was to determine the relationship between a new integrated parameter—both collateral circulation and relative permeability surface (PS)—and the hemorrhagic transformation (HT) in acute ischemic stroke (AIS) with middle cerebral artery occlusion (MCAO).

Methods

We retrospectively reviewed consecutive AIS patients with MCAO who underwent baseline CTA/CTP within 4 h of symptom onset and follow-up susceptibility-weighted imaging (SWI) within 3 weeks. Collateral circulation was assessed on the baseline CTA. Baseline CTP data were postprocessed to generate PS parameter. The patients with poor collateral circulation and at the same time with high relative PS were classified as the group of both poor collateral circulation and high relative PS. HT was defined according to European Cooperative Acute Stroke Study II criteria on follow-up SWI imaging. Multivariate logistic regression analysis was performed using HT as an outcome variable.

Results

The group of patients with both poor collateral circulation and high relative PS was thirteen and thirty-three (52%) developed HT of the final cohort sixty-three AIS patients with MCAO. Multivariate logistic analysis revealed the new integrated parameter—both collateral circulation and relative PS (odds ratio, 16.59; 95% confidence interval, 13.09–19.10; P < 0.001) was independent predictor of HT. The area under the curve was 0.85 (95% confidence interval, 0.81–0.89). The sensitivity was 57%, specificity 97% and positive predictive value 92%, negative predictive value 58%.

Conclusions

For AIS patients with MCAO, these with poor collateral circulation on CTA and at the same time with high relative PS on CTP were at high risk for HT.
Parents' Views of Family-Centered Care at a Pediatric Intensive Care Unit—A Qualitative Study
Authors: Terp Karina , Weis Janne , Lundqvist Pia
Published: August 2021

Purpose:To describe parents' views of family-centered care at a pediatric intensive care unit.

Design and Methods:A qualitative descriptive study with a deductive and inductive approach was conducted based on the principles of family-centered care. Inclusion criteria were parents of children cared for at a pediatric intensive care unit for at least 48 h. Parents of children who died during the hospital stay were excluded. The sample consisted of spontaneous responses from 70 parents to five open questions in the EMpowerment of PArents in THe Intensive Care questionnaire, which was completed at discharge. The spontaneous responses were analyzed using thematic analysis.

Results:The analysis of the parents' statement illuminated that partnership, the essence of family-centered care, appeared incomplete. Partnership was particularly evident regarding parents' experiences of being treated with empathy and respect. It also seemed prominent in situations where the professional team provided support to the child, parents, and family. Based on the parents' statements there was potential for development of the family-centered care approach in aspects such as decision-making concerning care and treatment, as well as improving person-centered communication on order to capture parents' experiences and needs in the highly technological pediatric intensive care unit environment.

Conclusions:Although in general parents were satisfied with the care, areas for improvement were identified such as participation in decision-making about care and treatment as well as person-centered communication. The results can contribute to future quality improvement interventions focusing family centered care at pediatric intensive care units.
Combining Electrospinning and Electrode Printing for the Fabrication of Stretchable Organic Electrochemical Transistors
Authors: Lerond Michael , Subramanian Arunprabaharan , Skene W. G. , Cicoira Fabio
Published: August 2021

Stretchable conductors and organic electrochemical transistors (OECT) were fabricated from PEDOT:Tos (poly (3,4-ethylenedioxythiophene):iron tosylate) nanofibers. The devices were prepared by a combination of electrospinning and electrode printing followed by vapor phase polymerization (VPP). The impact of both the processing time and the composition of three electrospinning mixtures on the electrospun fiber mats was evaluated by scanning electron microscopy and cyclic voltammetry. Fibrillar mats prepared from the different mixtures maintained their electrical properties and could be stretched up to 140% of their original length. Stretchable OECTs were fabricated by printing silver drain and source electrodes directly on the conductive spun fibers. The fabricated devices showed transistor behavior up to ∼50% strain.
Histological Analysis and Gene Expression of Satellite Cell Markers in the Pectoralis Major Muscle in Broiler Lines Divergently Selected for Percent 4-Day Breast Yield
Authors: Orlowski Sara K. , Dridi Sami , Greene Elizabeth S. , Coy Cynthia S. , Velleman Sandra G. , Anthony Nicholas B.
Published: August 2021

Muscle development during embryonic and early post-hatch growth is primarily through hyperplastic growth and accumulation of nuclei through satellite cell contribution. Post-hatch, muscle development transitions from hyperplasia to hypertrophic growth of muscle fibers. Commercial selection for breast yield traditionally occurs at ages targeting hypertrophic rather than hyperplastic growth. This has resulted in the production of giant fibers and concomitant challenges with regard to muscle myopathies. The current study investigates the impact of selection during the period of hyperplastic growth. It is hypothesized that selection for percentage breast yield during hyperplasia will result in an increased number of muscle cells at hatch and potentially impact muscle fiber characteristics at processing. This study characterizes the breast muscle histology of three broiler lines at various ages in the growth period. The lines include a random bred control (RAN) as well as lines which have been selected from RAN for high (HBY4) and low (LBY4) percentage 4-day breast yield. Post-rigor pectoralis major samples from six males of each line and age were collected and stored in formalin. The sample ages included embryonic day 18 (E18), post-hatch day 4 (d4), and day 56 (d56). The samples were processed using a Leica tissue processor, embedded in paraffin wax, sectioned, and placed on slides. Slides were stained using hematoxylin and eosin. E18 and d4 post-hatch analysis showed advanced muscle fiber formation for HBY4 and immature muscle development for LBY4 as compared to RAN. Post-hatch d56 samples were analyzed for fiber number, fiber diameter, endomysium, and perimysium spacing. Line HBY4 had the largest muscle fiber diameter (54.2 ± 0.96 μm) when compared to LBY4 (45.4 ± 0.96 μm). There was no line difference in endomysium spacing while perimysium spacing was higher for HBY4 males. Selection for percentage 4-day breast yield has impacted the rate and extent of muscle fiber formation in both the LBY4 and HBY4 lines with no negative impact on fiber spacing. The shift in processing age to later ages has exposed issues associated with muscle fiber viability. Selection during the period of muscle hyperplasia may impact growth rate; however, the potential benefits of additional satellite cells are still unclear.
After the Revolution: State, Civil Society, and Democratization in Armenia and Georgia
Authors: Stefes Christoph H. , Paturyan Yevgenya J.
Published: August 2021

In 2003 and 2018, mass protests triggered the collapse of authoritarian regimes in Georgia and Armenia, respectively. In both cases, civil society organizations (CSOs) played an important role in laying the groundwork and organizing the protests. Following the toppling of semi-autocratic leaders, reform-oriented governments took over in both countries. Yet, the way civil society engaged the new rulers differed considerably. Whereas in Georgia, former civil society leaders were often absorbed into the new government, Armenian civil society has kept its distance from the new political leadership. In this paper, we attempt to explain why state-civil society relations after the revolutions have developed in different directions in these two Soviet successor states. We argue that three conditions explain differences in engagement with the new governments: CSOs pre-revolutionary cooperation with the political opposition, Western governments support for civil society before and after the political transitions, and the degree to which CSOs represent and are rooted in the general public. As a consequence, Georgia’s post-revolutionary regime lacked the checks and balances that CSOs usually provide, allowing it to sacrifice democratization on the altar of modernization. In Armenia, in contrast, CSOs have maintained a critical stance and continued to hold the government accountable.
Equity Structure, Strategic Investment Psychology, and Performance in China’s Green Economy Context
Authors: Guo Li Xin , Lin Kuen-Lin , Zhang Li-Ting , Liu Chi-Fang
Published: August 2021

This study empirically tests the impacts of equity structure on strategic investment psychology in green affairs in R&D vs. Marketing dimensions and company performance. Based on data from Chinese high-tech industry listed companies, the empirical results show that: (1) the largest shareholder’s shareholding ratio has a positive effect on marketing investment psychology and a negative impact on R&D investment psychology, (2) other large shareholders’ shareholding ratio are positive related to R&D investment psychology; (3) R&D investment psychology has a negative effect and marketing investment psychology has a positive influence on the current performance; (4) equity counterbalance is positive related to R&D investment psychology and has a negative effect on the current performance. This study contributes to the literature of corporate governance on sustainability issue by providing a new psychological perspective. The results also provide an important guidance for the corporate governance practice in green economies.
Automatically Generated Smartphone Data in Young Patients With Newly Diagnosed Bipolar Disorder and Healthy Controls
Authors: Melbye Sigurd , Stanislaus Sharleny , Vinberg Maj , Frost Mads , Bardram Jakob Eyvind , Kessing Lars Vedel , Faurholt-Jepsen Maria
Published: August 2021

Background: Smartphones may facilitate continuous and fine-grained monitoring of behavioral activities via automatically generated data and could prove to be especially valuable in monitoring illness activity in young patients with bipolar disorder (BD), who often present with rapid changes in mood and related symptoms. The present pilot study in young patients with newly diagnosed BD and healthy controls (HC) aimed to (1) validate automatically generated smartphone data reflecting physical and social activity and phone usage against validated clinical rating scales and questionnaires; (2) investigate differences in automatically generated smartphone data between young patients with newly diagnosed BD and HC; and (3) investigate associations between automatically generated smartphone data and smartphone-based self-monitored mood and activity in young patients with newly diagnosed BD.

Methods: A total of 40 young patients with newly diagnosed BD and 21 HC aged 15–25 years provided daily automatically generated smartphone data for 3–779 days [median (IQR) = 140 (11.5–268.5)], in addition to daily smartphone-based self-monitoring of activity and mood. All participants were assessed with clinical rating scales.

Results: (1) The number of outgoing phone calls was positively associated with scores on the Young Mania Rating Scale and subitems concerning activity and speech. The number of missed calls (p = 0.015) and the number of outgoing text messages (p = 0.017) were positively associated with the level of psychomotor agitation according to the Hamilton Depression Rating scale subitem 9. (2) Young patients with newly diagnosed BD had a higher number of incoming calls compared with HC (BD: mean = 1.419, 95% CI: 1.162, 1.677; HC: mean = 0.972, 95% CI: 0.637, 1.308; p = 0.043) and lower self-monitored mood and activity (p's < 0.001). (3) Smartphone-based self-monitored mood and activity were positively associated with step counts and the number of outgoing calls, respectively (p's < 0.001).

Conclusion: Automatically generated data on physical and social activity and phone usage seem to reflect symptoms. These data differ between young patients with newly diagnosed BD and HC and reflect changes in illness activity in young patients with BD. Automatically generated smartphone-based data could be a useful clinical tool in diagnosing and monitoring illness activity in young patients with BD.
Drug-Induced Stuttering: Occurrence and Possible Pathways
Authors: Ekhart Corine , van Hunsel Florence , van Harten Peter , van Baarsen Jeanette , Yingying Tan , Bast Bert
Published: August 2021

Background: Stuttering is a well-known condition that affects mainly children. Often, they recover as they get older. However, a drug-induced form of stuttering may occur at any age. The aim of the present study was to detect drugs that have been associated with stuttering and discuss the mechanisms involved.

Method: A descriptive study based on reports submitted to the global pharmacovigilance database VigiBase of the WHO was conducted.

Results: A total of 3,385 reports of dysphemia were retrieved from VigiBase. These reports were contributed by 51 countries. Antiepileptics, antidepressants, immunosuppressants, antipsychotics, and centrally acting sympathomimetics were among the most frequently implicated drugs.

Conclusion: A wide variety of drugs has been linked to the occurrence or recurrence of stuttering. Several mechanisms, such as increased dopamine levels, reduction of GABA, anticholinergic properties of drugs, or changes in serotonin levels, have been associated with the development of drug-induced stuttering. Paradoxically, agents known to reduce stuttering in some people may induce it in others.
Examination of the Association Between Access to Care and Lung Cancer Screening Among High-Risk Smokers
Authors: Li Chien-Ching , Matthews Alicia K. , Kao Yu-Hsiang , Lin Wei-Ting , Bahhur Jad , Dowling Linda
Published: August 2021

Objective: The purpose of this study was to examine the influence of access to care on the uptake of low-dose computed tomography (LDCT) lung cancer screening among a diverse sample of screening-eligible patients.

Methods: We utilized a cross-sectional study design. Our sample included patients evaluated for lung cancer screening at a large academic medical center (AMC) between 2015 and 2017 who met 2013 USPSTF guidelines for LDCT screening eligibility. The completion of LDCT screening (yes, no) was the primary dependent variable. The independent variable was access to care (insurance type, living within the AMC service area). We utilized binary logistic regression analyses to examine the influence of access to care on screening completion after adjusting for demographic factors (age, sex, race) and smoking history (current smoking status, smoking pack-year history).

Results: A total of 1,355 individuals met LDCT eligibility criteria, and of those, 29.8% (n = 404) completed screening. Regression analysis results showed individuals with Medicaid insurance (OR, 1.51; 95% CI, 1.03-2.22), individuals living within the AMC service area (OR, 1.71; 95% CI, 1.21–2.40), and those aged 65–74 years (OR, 1.49; 95% CI, 1.12–1.98) had higher odds of receiving LDCT lung cancer screening. Lower odds of screening were associated with having Medicare insurance (OR, 0.30; 95% CI, 0.22–0.41) and out-of-pocket (OR, 0.27; 95% CI, 0.15–0.47).

Conclusion: Access to care was independently associated with lowered screening rates. Study results are consistent with prior research identifying the importance of access factors on uptake of cancer early detection screening behaviors.
Legal Profession and Corruption in Health Care: Some Reflective Realities in South Africa
Authors: Mantzaris Evangelos , Pillay Pregala
Published: August 2021

The article is an empirical attempt to research, analyse, and dissect the corrupt involvement of legal practitioners in illegal and fraudulent acts, and mainly their involvement in litigation associated with issues of medical negligence. This is done primarily but not exclusively through the utilisation of several qualitative research methods, including the content analysis of primary literary sources such as official state documents, existing legislation and court proceedings and personal interviews with senior public servants, as well as secondary sources. Beginning with a short exploration of South Africa's public legal terrain and the fears of sections of the statutory leadership of the legal profession, the article continues with the identification of key findings in several the country's provinces, the modus operandi of the corrupt individuals and groups, as well as the monetary, financial and social repercussions of such actions.
Agrochemical Usage Inventory and Measured Residues in Streams Within an Off-Season Durian Production Region of Thailand
Authors: Pibul Panatda , Jawjit Siriuma
Published: August 2021

Rising demand for off-season durian production in Thailand has led to increased agrichemical use and potential environmental and health concerns. In this study, we surveyed 117 farmers and collected water samples from 11 villages in Krungching Sub-district. Overall, 108 agrochemicals were listed as being used in off-season durian production, but we focused on residual concentrations of the persistent herbicides glyphosate and paraquat in surface water. We applied a pollution release and transfer registry for 2016 to determine an application rate of 288,149 kg/year for these agrichemicals. These were primarily detected during the dry season, and not the wet season. This reporting system can be used to develop seasonal environmental surveillance and monitoring tools for more sustainable use of agrochemical substances in off-season durian production in environmentally sensitive areas such as the Krungching River watershed, especially during the first stage of cultivation (dry season), in order to ensure the use of agricultural chemicals under appropriate circumstances. Further studies are needed to better understand the full diversity of persistent agrochemical substance usage and disposal, in order to reorient agricultural activities to focus on local needs and capabilities as part of efforts to implement more sustainable agriculture in Thailand.
Development of a Numerical Model to Simulate Laser-Shock Paint Stripping on Aluminum Substrates
Authors: Tserpes Konstantinos , Papadopoulos Kosmas , Unaldi Selen , Berthe Laurent
Published: August 2021

An explicit 3D Finite Element (FE) model was developed in the LS-Dyna code to simulate the laser shock paint stripping on aircraft aluminum substrates. The main objective of the model is to explain the physical mechanisms of the laser shock stripping process in terms of shock wave propagation, stress and strain evolution and stripping shape and size and to evaluate the effects of laser and material parameters on the stripping pattern. To simulate the behavior of aluminum, the Johnson–Cook plasticity model and the Gruneisen equation of state were applied. To simulate stripping, the cohesive zone modeling method was applied. The FE model was compared successfully against experiments in terms of back-face velocity profiles. The parameters considered in the study are the aluminum thickness, the epoxy paint thickness, the laser spot diameter, the fracture toughness of the aluminum/epoxy interface and the maximum applied pressure. In all cases, a circular solid or hollow stripping pattern was predicted, which agrees with the experimental findings. All parameters were found to affect the stripping pattern. The numerical results could be used for the design of selective laser shock stripping tests.
Chronic Stress Detrimentally Affects In Vivo Maturation in Rat Oocytes and Oocyte Viability at All Phases of the Estrous Cycle
Authors: Casillas Fahiel , Betancourt Miguel , Juárez-Rojas Lizbeth , Ducolomb Yvonne , López Alma , Ávila-Quintero Alejandra , Zamora Jimena , Ommati Mohammad Mehdi , Retana-Márquez Socorro
Published: August 2021

Background: Stress has been considered as one of the causes of decreased reproductive function in women. However, direct evidence of the effect of chronic stress on oocytes depending on estrous cycle phases is limited. Objective: The present study aimed to evaluate the impact of chronic stress on the viability, integrity, and maturation of rat oocytes depending on estrous cycle phases, specifically proestrus, estrus, and diestrus. Methods: For this purpose, adult female rats were stressed daily by cold water immersion (15 °C) for 30 consecutive days. Results: In chronically stressed female rats, irregular estrous cyclicity, increased corticosterone levels, decreased oocyte viability, and an increased percentage of abnormal oocytes were obtained in all the estrous cycle phases, resulting in reduced oocyte maturation during proestrus. Conclusion: Oocyte maturation disturbed by chronic stress is a crucial factor by which chronic stress disrupts female reproduction
Wider Angle Egg Turning during Incubation Enhances Yolk Utilization and Promotes Goose Embryo Development
Authors: Guo Binbin , Yan Leyan , Lei Mingming , Dai Zichun , Shi Zhendan
Published: August 2021

We aimed to investigate how wide-angle turning of eggs during incubation affected yolk utilization and the associated molecular mechanism, along with improved goose embryonic development. In total, 1152 eggs (mean weight: 143.33 ± 5.43 g) were divided equally and incubated in two commercial incubators with tray turning angles adjusted differently, to either 50° or 70°. Following incubation under the standard temperature and humidity level, turning eggs by 70° increased embryonic days 22 (E22), embryo mass, gosling weight at hatching, and egg hatchability, but reduced E22 yolk mass compared with those after turning eggs by 50°. Lipidomic analyses of the yolk revealed that egg turning at 70° reduced the concentrations of 17 of 1132 detected total lipids, including diglycerides, triglycerides, and phospholipids. Furthermore, the 70° egg turning upregulated the expression of genes related to lipolysis and fat digestion enzymes, such as lipase, cathepsin B, and prosaposin, as well as apolipoprotein B, apolipoprotein A4, very low-density lipoprotein receptor, low-density lipoprotein receptor-related protein 2, and thrombospondin receptor, which are genes involved in lipid transportation. Thus, a 70° egg turning angle during incubation enhances yolk utilization through the upregulation of lipolysis and fat digestion-related gene expression, thereby promoting embryonic development and improving egg hatchability and gosling quality.
Prevalence of Neospora caninum Exposure in Wild Pigs (Sus scrofa) from Oklahoma with Implications of Testing Method on Detection
Authors: Haydett Katelyn M. , Peper Steven T. , Reinoso Webb Cynthia , Tiffin Hannah S. , Wilson-Fallon Alexander N. , Jones-Hall Yava L. , Webb Stephen L. , Presley Steven M.
Published: August 2021

Neospora caninum is a protozoan parasite, reported as a leading cause of cattle abortions and reproductive failure worldwide, costing the cattle industry approximately $1.3 billion annually. With wild pig (Sus scrofa) populations estimated at over six million in the United States, contact between wild pigs and livestock is inevitable, mainly because of the widespread geographic co-occurrence of the two species. As a known reservoir for numerous fungal, bacterial, viral and parasitic diseases, wild pigs are of particular importance for human and veterinary health relative to the prevention of infectious diseases. The seroprevalence of N. caninum in wild pig populations was previously documented in the United States, raising the question as to their exposure point of prevalence. This research screened 116 individual wild pigs for N. caninum using a variety of available assays. Using two different commercially available ELISA test kits, seroprevalence ranged from 12.5% to 67.8%. The Indirect Fluorescent Antibody Test resulted in our highest percent seroprevalence for these samples, at 84.1%. However, none of our samples showed any presence of N. caninum or associated pathologies via histological evaluation of representative tissues. Importantly, the assays used in this study were not congruent with all duplicate samples or between the test types used. The implications of these non-congruent results demonstrates that currently available testing assays produce variable results, underscoring the need for more reliable testing kits and a standardized methodology when assessing disease prevalence in wildlife, particularly for N. caninum in wild pigs, which impacts prevalence and comparability across studies.