bluuebunny/crossref_metadata_2025 huggingface dataset:
Subjective Psychophysical Experiences in the Course of Inflammatory Bowel Disease—A Comparative Analysis Based on the Polish Pediatric Crohn’s and Colitis Cohort (POCOCO)
Authors: Glapa-Nowak Aleksandra , Bukowska-Posadzy Anna , Szczepanik Mariusz , Kwiecień Jarosław , Szaflarska-Popławska Anna , Iwańczak Barbara , Flak-Wancerz Anna , Dembiński Łukasz , Osiecki Marcin , Kierkuś Jarosław , Banasiewicz Tomasz , Walach Harald , Banaszkiewicz Aleksandra , Walkowiak Jarosław
Published: January 2021

No gold standard is available to evaluate subjective psychophysical experiences in pediatric inflammatory bowel disease (IBD). We aimed to assess pain, anxiety, and limitations in social activities at diagnosis and the worst flare of the disease in relation to clinical expression, treatment and IBD severity. A total of 376 children completed the survey (Crohn’s disease (CD) n = 196; ulcerative colitis (UC) n = 180). The questionnaire included 12 questions regarding pain, anxiety, and social activity, all assessed at recruitment and retrospectively at diagnosis and worst flare using a numeric rating scale. Patients that had ever been treated with systemic glucocorticosteroids scored higher in pain (p < 0.001), anxiety (p = 0.015), and social activity domains (p < 0.016) at worst flare, and the answers correlated with the number of steroid courses (p < 0.0392). The perception of social activity limitations also correlated independently with the number of immunosuppressants (p < 0.0433) and biological agents (p < 0.0494). There was no difference in retrospective perception of pain, anxiety and social activity limitations between CD and UC patients at diagnosis and the worst flare. The level of limitations in social activity correlated with hospitalisations due to relapse, days spent in the hospital, number of relapses, and severe relapses with the strongest association of rho = 0.39 (p = 0.0004). Subjective and retrospective perception of pain, anxiety, and limitations in social activity differs depending on therapy, correlates with treatment modalities, and severity measures such as hospitalisations.
Relationship between Lower Urinary Tract Symptoms and Treatment-Related Behavior in an Eastern European Country: Findings from the LUTS POLAND Study
Authors: Przydacz Mikolaj , Dudek Przemyslaw , Golabek Tomasz , Chlosta Piotr
Published: January 2021

Background: The aim of this study was to investigate the effect of lower urinary tract symptoms (LUTS) on behavior related to treatment of Polish adults aged ≥ 40 years. Methods: We conducted a computer-assisted telephone survey with a study sample stratified by age, sex, and place of residence (type, size, urban versus rural) reflecting the entire Polish population. Participants rated the frequency and symptom-specific bother of individual LUTS and their effects on seeking and receiving treatment, treatment satisfaction, and treatment continuation. We adjusted multiple logistic regression models to analyze the simultaneous effects of predictor variables on each dependent variable. Results: Overall, 6005 participants completed the interview. One third (29.6–33.5%) of participants with LUTS were seeking treatment, and 24.0–26.4% received treatment. There was no difference in treatment seeking and receiving between urban and rural areas. Whereas storage and voiding symptoms were significantly related to treatment seeking by both men and women, treatment receiving correlated only with voiding symptoms in men and only with storage symptoms in women. Most respondents who received treatment were satisfied; treatment dissatisfaction was related to the presence of storage symptoms in both men and women. Only 50% of all participants continued their treatment; discontinuation of treatment was statistically more prevalent for women than for men. Conclusion: This investigation, the first population-representative study performed in Eastern Europe, revealed a low frequency of seeking treatment for LUTS. In addition, symptoms that inclined participants to seek treatment might not have been adequately addressed by the treatment they received. We also found a relatively high rate of treatment discontinuation. Clearly, there is a need for both improved patient education about LUTS treatment and a need for increased clinician awareness of the coexistence of different symptoms in men and women plus proactive evaluation by physicians for all types of LUTS and associated bother.
The Motivations for and Well-Being Implications of Social Media Use at Work among Millennials and Members of Former Generations
Authors: Oksa Reetta , Saari Tiina , Kaakinen Markus , Oksanen Atte
Published: January 2021

Working life has digitalized considerably in recent decades and organizations have taken into use new forms of collaborative technologies such as social media platforms. This study examined the relationship between social media use at work and well-being at work for millennials and members of former generations in Finland. The research data contained focus group interviews (N = 52), an expert organization survey (N = 563), and a nationally representative survey (N = 1817). Well-being measures included technostress, burnout, psychological distress, and a set of background variables. Content analysis and linear regression models were used as analysis methods. The results showed that millennials have various intrinsic and extrinsic motivations for social media use at work. Intrinsic motivations included employees’ personal choice and their pure interest to follow the market and discussions in their own field. Extrinsic motivations were related mainly to organizations’ work culture and personal branding. The survey findings revealed, however, that millennials were not only more active social media users for work, but they also experienced higher technostress and burnout than members of former generations. Social media use motivations were associated with both higher and lower technostress and burnout depending on motivation, indicating that social media use can have both positive and negative effects. Overall, our findings suggest that employees tend to utilize social media more if their needs for autonomy, competence, and relatedness are fulfilled.
Depressive Symptoms, Fatigue and Social Relationships Influenced Physical Activity in Frail Older Community-Dwellers during the Spanish Lockdown due to the COVID-19 Pandemic
Authors: Pérez Laura M. , Castellano-Tejedor Carmina , Cesari Matteo , Soto-Bagaria Luis , Ars Joan , Zambom-Ferraresi Fabricio , Baró Sonia , Díaz-Gallego Francisco , Vilaró Jordi , Enfedaque María B. , Espí-Valbé Paula , Inzitari Marco
Published: January 2021

Due to the dramatic impact of the COVID-19 pandemic, Spain underwent a strict lockdown (March–May 2020). How the lockdown modified older adults’ physical activity (PA) has been poorly described. This research assesses the effect of the lockdown on PA levels and identifies predictors of sufficient/insufficient PA in frail older community-dwellers. Community-dwelling participants from the +ÀGIL Barcelona frailty intervention program, suspended during the pandemic, underwent a phone-assessment during the lockdown. PA was measured before and after the lockdown using the Brief Physical Activity Assessment Tool (BPAAT). We included 98 frail older adults free of COVID-19 (mean age = 82.7 years, 66.3% women, mean Short Physical Performance Battery = 8.1 points). About one third of participants (32.2%) were not meeting sufficient PA levels at the end of the lockdown. Depressive symptoms (OR = 0.12, CI95% = 0.02–0.55) and fatigue (OR = 0.11, CI95% = 0.03–0.44) decreased the odds of maintaining sufficient PA, whereas maintaining social networks (OR = 5.07, CI95% = 1.60–16.08) and reading (OR = 6.29, CI95% = 1.66–23.90) increased it. Living alone was associated with the reduction of PA levels (b = −1.30, CI95% = −2.14–−0.46). In our sample, pre-lockdown mental health, frailty-related symptoms and social relationships were consistently associated with both PA levels during-lockdown and pre-post change. These data suggest considering specific plans to maintain PA levels in frail older community-dwellers.
Hybrid Decision Support to Monitor Atrial Fibrillation for Stroke Prevention
Authors: Lei Ningrong , Kareem Murtadha , Moon Seung Ki , Ciaccio Edward J. , Acharya U Rajendra , Faust Oliver
Published: January 2021

In this paper, we discuss hybrid decision support to monitor atrial fibrillation for stroke prevention. Hybrid decision support takes the form of human experts and machine algorithms working cooperatively on a diagnosis. The link to stroke prevention comes from the fact that patients with Atrial Fibrillation (AF) have a fivefold increased stroke risk. Early diagnosis, which leads to adequate AF treatment, can decrease the stroke risk by 66% and thereby prevent stroke. The monitoring service is based on Heart Rate (HR) measurements. The resulting signals are communicated and stored with Internet of Things (IoT) technology. A Deep Learning (DL) algorithm automatically estimates the AF probability. Based on this technology, we can offer four distinct services to healthcare providers: (1) universal access to patient data; (2) automated AF detection and alarm; (3) physician support; and (4) feedback channels. These four services create an environment where physicians can work symbiotically with machine algorithms to establish and communicate a high quality AF diagnosis.
Personal and Work-Related Factors Associated with Good Care for Institutionalized Older Adults
Authors: López Javier , Pérez-Rojo Gema , Noriega Cristina , Velasco Cristina
Published: January 2021

Despite efforts to promote good care, many institutionalized older adults (IOA) experience elevated neglectful conditions and reduced person-centered care approaches. Based on the job demand–control model, this study aimed to analyze the relationship between nursing home professionals’ personal and organizational factors and good care provided to institutionalized older people. Data was collected through a self-administered survey completed by 208 nursing home staff members. Three dimensions of personal factors (i.e., personal accomplishment, depersonalization, and negative old age stereotypes) were significant predictors of good care. Depersonalization and negative old age stereotypes were negatively associated with IOA, and both good care and personal accomplishment were positively associated with good care in nursing homes. Only one work-related factor (i.e., management support) was positively associated with good care. Personal factors may play a significant role in good care. Management support offers a promising mechanism to promote good care among nursing home professionals. The findings support the need to change the focus on entirely completing care tasks to providing good care of residents in nursing homes that promotes management support, personal accomplishment, personalization and positive old age consideration, attitudes, and behaviors. Policies and interventions should be developed to address in a more humanized way.
The Influence of Child-Related Factors on Caregiver Perceptions of Their Child’s Sustained Participation in a Community Football Program: A Study of Children with and without Neurodevelopmental Disorders
Authors: Sivaratnam Carmel , Devenish Bethany , Chellew Tayla , Papadopoulos Nicole , McGillivray Jane , Rinehart Nicole
Published: January 2021

This study evaluated the influence of activity preference and involvement on season completion in a community-based football program for children with and without neurodevelopmental disorders. Caregivers (n = 1428) of 1529 children aged 4 to 17 (M = 7.27, SD = 1.85), with (n = 175) and without (n = 1354) neurodevelopmental disorders who were currently participating or had previously participated in a group-based NAB AFL Auskick football program completed an online survey. The survey collected information on their child’s completion of any attempted seasons of the football program, level of involvement during the sessions and preference for football over other sports and activities. Eighty percent of children with a neurodevelopmental diagnosis had completed all seasons of Auskick, compared with 93% of children without a neurodevelopmental diagnosis. Results indicated that children with neurodevelopmental disorders (n = 135) were 3.71 times less likely to complete a football season than their typically developing peers (n = 903). Higher levels of involvement during football sessions and greater preference for football were linked to a higher football season completion rate, irrespective of neurodevelopmental disability diagnosis. This study highlights the influence of child-related factors, in particular, preference and involvement, on children’s sustained participation in community football programs, regardless of neurodevelopmental disability status.
The Significant Association between Health Examination Results and Population Health: A Cross-Sectional Ecological Study Using a Nation-Wide Health Checkup Database in Japan
Authors: Nagakura Yukinori , Kato Hideaki , Asano Satoshi , Jinno Yasuhiro , Tanei Shigeharu
Published: January 2021

In Japan, population health with life expectancy (LE) and healthy life expectancy (HALE) as indicators varies across the 47 prefectures (administrative regions). This study investigates how health examination results, including attitude toward improving life habits, are associated with population health. The association between health checkup variables and summary population health outcomes (i.e., life expectancy and healthy life expectancy) was investigated using a cross-sectional ecological design with prefectures as the unit of analysis. The medical records, aggregated by prefecture, gender, and age in the National Database of Health Insurance Claims and Specific Health Checkups of Japan (NDB) Open Data Japan, were used as health checkup variables. Body weight, blood pressure, liver enzymes, drinking habits, smoking habits, diabetes, serum lipids, and answers to questions regarding attitude toward improving health habits were significantly correlated to population health outcomes. Multiple regression analysis also revealed significant influence of these variables on population health. This study highlights that health examination results, including attitude toward improving health habits, are positively associated with population health. Consequently, implementing measures to improve health habits in response to the examination results could help the population maintain a healthy life.
From Posttranslational Modifications to Disease Phenotype: A Substrate Selection Hypothesis in Neurodegenerative Diseases
Authors: Baskakov Ilia V.
Published: January 2021

A number of neurodegenerative diseases including prion diseases, tauopathies and synucleinopathies exhibit multiple clinical phenotypes. A diversity of clinical phenotypes has been attributed to the ability of amyloidogenic proteins associated with a particular disease to acquire multiple, conformationally distinct, self-replicating states referred to as strains. Structural diversity of strains formed by tau, α-synuclein or prion proteins has been well documented. However, the question how different strains formed by the same protein elicit different clinical phenotypes remains poorly understood. The current article reviews emerging evidence suggesting that posttranslational modifications are important players in defining strain-specific structures and disease phenotypes. This article put forward a new hypothesis referred to as substrate selection hypothesis, according to which individual strains selectively recruit protein isoforms with a subset of posttranslational modifications that fit into strain-specific structures. Moreover, it is proposed that as a result of selective recruitment, strain-specific patterns of posttranslational modifications are formed, giving rise to unique disease phenotypes. Future studies should define whether cell-, region- and age-specific differences in metabolism of posttranslational modifications play a causative role in dictating strain identity and structural diversity of strains of sporadic origin.
In Vitro Macrophage Immunomodulation by Poly(ε-caprolactone) Based-Coated AZ31 Mg Alloy
Authors: Negrescu Andreea-Mariana , Necula Madalina-Georgiana , Gebaur Adi , Golgovici Florentina , Nica Cristina , Curti Filis , Iovu Horia , Costache Marieta , Cimpean Anisoara
Published: January 2021

Due to its excellent bone-like mechanical properties and non-toxicity, magnesium (Mg) and its alloys have attracted great interest as biomaterials for orthopaedic applications. However, their fast degradation rate in physiological environments leads to an acute inflammatory response, restricting their use as biodegradable metallic implants. Endowing Mg-based biomaterials with immunomodulatory properties can help trigger a desired immune response capable of supporting a favorable healing process. In this study, electrospun poly(ε-caprolactone) (PCL) fibers loaded with coumarin (CM) and/or zinc oxide nanoparticles (ZnO) were used to coat the commercial AZ31 Mg alloy as single and combined formulas, and their effects on the macrophage inflammatory response and osteoclastogenic process were investigated by indirect contact studies. Likewise, the capacity of the analyzed samples to generate reactive oxygen species (ROS) has been investigated. The data obtained by attenuated total reflection Fourier-transform infrared (FTIR-ATR) and X-ray photoelectron spectroscopy (XPS) analyses indicate that AZ31 alloy was perfectly coated with the PCL fibers loaded with CM and ZnO, which had an important influence on tuning the release of the active ingredient. Furthermore, in terms of degradation in phosphate-buffered saline (PBS) solution, the PCL-ZnO- and secondary PCL-CM-ZnO-coated samples exhibited the best corrosion behaviour. The in vitro results showed the PCL-CM-ZnO and, to a lower extent, PCL-ZnO coated sample exhibited the best behaviour in terms of inflammatory response and receptor activator of nuclear factor kappa-B ligand (RANKL)-mediated differentiation of RAW 264.7 macrophages into osteoclasts. Altogether, the results obtained suggest that the coating of Mg alloys with fibrous PCL containing CM and/or ZnO can constitute a feasible strategy for biomedical applications.
Prebiotic Organic Chemistry of Formamide and the Origin of Life in Planetary Conditions: What We Know and What Is the Future
Authors: Bizzarri Bruno Mattia , Saladino Raffaele , Delfino Ines , García-Ruiz Juan Manuel , Di Mauro Ernesto
Published: January 2021

The goal of prebiotic chemistry is the depiction of molecular evolution events preceding the emergence of life on Earth or elsewhere in the cosmos. Plausible experimental models require geochemical scenarios and robust chemistry. Today we know that the chemical and physical conditions for life to flourish on Earth were at work much earlier than thought, i.e., earlier than 4.4 billion years ago. In recent years, a geochemical model for the first five hundred million years of the history of our planet has been devised that would work as a cradle for life. Serpentinization processes in the Hadean eon affording self-assembled structures and vesicles provides the link between the catalytic properties of the inorganic environment and the impressive chemical potential of formamide to produce complete panels of organic molecules relevant in pre-genetic and pre-metabolic processes. Based on an interdisciplinary approach, we propose basic transformations connecting geochemistry to the chemistry of formamide, and we hint at the possible extension of this perspective to other worlds.
Advances in Research of Adult Gliomas
Authors: Finch Alina , Solomou Georgios , Wykes Victoria , Pohl Ute , Bardella Chiara , Watts Colin
Published: January 2021

Diffuse gliomas are the most frequent brain tumours, representing 75% of all primary malignant brain tumours in adults. Because of their locally aggressive behaviour and the fact that they cannot be cured by current therapies, they represent one of the most devastating cancers. The present review summarises recent advances in our understanding of glioma development and progression by use of various in vitro and in vivo models, as well as more complex techniques including cultures of 3D organoids and organotypic slices. We discuss the progress that has been made in understanding glioma heterogeneity, alteration in gene expression and DNA methylation, as well as advances in various in silico models. Lastly current treatment options and future clinical trials, which aim to improve early diagnosis and disease monitoring, are also discussed.
Angiogenesis in Regenerative Dentistry: Are We Far Enough for Therapy?
Authors: Baru Oana , Nutu Andreea , Braicu Cornelia , Cismaru Cosmin Andrei , Berindan-Neagoe Ioana , Buduru Smaranda , Badea Mîndra
Published: January 2021

Angiogenesis is a broad spread term of high interest in regenerative medicine and tissue engineering including the dental field. In the last two decades, researchers worldwide struggled to find the best ways to accelerate healing, stimulate soft, and hard tissue remodeling. Stem cells, growth factors, pathways, signals, receptors, genetics are just a few words that describe this area in medicine. Dental implants, bone and soft tissue regeneration using autologous grafts, or xenografts, allografts, their integration and acceptance rely on their material properties. However, the host response, through its vascularization, plays a significant role. The present paper aims to analyze and organize the latest information about the available dental stem cells, the types of growth factors with pro-angiogenic effect and the possible therapeutic effect of enhanced angiogenesis in regenerative dentistry.
Platelet-Rich Plasma Promotes the Proliferation of Human Keratinocytes via a Progression of the Cell Cycle. A Role of Prolidase
Authors: Misiura Magdalena , Guszczyn Tomasz , Oscilowska Ilona , Baszanowska Weronika , Palka Jerzy , Miltyk Wojciech
Published: January 2021

Although the role of platelet-rich plasma (PRP) in tissue regeneration has been confirmed in many studies, the mechanism of this process is still not fully understood. Human keratinocytes (HaCaT) cells were used as an experimental model for studies on the effects of PRP on cell proliferation, migration, collagen biosynthesis, prolidase activity, and its expression and anabolic signaling. The activation of epidermal growth factor receptor (EGFR), β1-integrin, and insulin-like growth factor-1 receptor (IGF-1R) by PRP were investigated by western blot and immunocytochemistry. It has been found that PRP induced keratinocytes migration and proliferation through activation of cell cycle progression and EGFR downstream signaling. Similar biological effects were achieved by an addition to the culture medium of prolidase (PEPD), a ligand of EGFR (PRP is a rich source of PEPD–2 ng/mL). PRP-dependent stimulation of collagen biosynthesis was accompanied by an increase in the expression of NF-κβ, IGF-1R-downstream signaling proteins, and PEPD activity. The data suggest that PRP activates a complex of growth factors and adhesion receptors that stimulate cell proliferation, migration, and collagen biosynthesis. PRP induces PEPD-dependent human keratinocyte proliferation through activation of the EGFR receptor. Our study provides a novel mechanism of PRP-dependent wound healing.
Macrophages in Healing Wounds: Paradoxes and Paradigms
Authors: DiPietro Luisa A. , Wilgus Traci A. , Koh Timothy J.
Published: January 2021

Macrophages are prominent cells in normally healing adult skin wounds, yet their exact functions and functional significance to healing outcomes remain enigmatic. Many functional attributes are ascribed to wound macrophages, including host defense and support of the proliferation of new tissue to replace that lost by injury. Indeed, the depletion of macrophages is unmistakably detrimental to normal skin healing in adult mammals. Yet in certain systems, dermal wounds seem to heal well with limited or even no functional macrophages, creating an apparent paradox regarding the function of this cell in wounds. Recent advances in our understanding of wound macrophage phenotypes, along with new information about cellular plasticity in wounds, may provide some explanation for the apparently contradictory findings and suggest new paradigms regarding macrophage function in wounds. Continued study of this remarkable cell is needed to develop effective therapeutic options to improve healing outcomes.
The Bioavailability, Extraction, Biosynthesis and Distribution of Natural Dihydrochalcone: Phloridzin
Authors: Tian Lei , Cao Jianxin , Zhao Tianrui , Liu Yaping , Khan Afsar , Cheng Guiguang
Published: January 2021

Phloridzin is an important phytochemical which was first isolated from the bark of apple trees. It is a member of the dihydrochalcones and mainly distributed in the plants of the Malus genus, therefore, the extraction method of phloridzin was similar to those of other phenolic substances. High-speed countercurrent chromatography (HSCCC), resin adsorption technology and preparative high-performance liquid chromatography (HPLC) were used to separate and purify phloridzin. Many studies showed that phloridzin had multiple pharmacological effects, such as antidiabetic, anti-inflammatory, antihyperglycaemic, anticancer and antibacterial activities. Besides, the physiological activities of phloridzin are cardioprotective, neuroprotective, hepatoprotective, immunomodulatory, antiobesity, antioxidant and so on. The present review summarizes the biosynthesis, distribution, extraction and bioavailability of the natural compound phloridzin and discusses its applications in food and medicine.
Natural Polyhydroxy Flavonoids, Curcuminoids, and Synthetic Curcumin Analogs as α7 nAChRs Positive Allosteric Modulators
Authors: Ximenis Marta , Mulet José , Sala Salvador , Sala Francisco , Criado Manuel , González-Muñiz Rosario , Pérez de Vega María Jesús
Published: January 2021

The α7 nicotinic acetylcholine receptor (α7 nAChR) is a ligand-gated ion channel that is involved in cognition disorders, schizophrenia, pain, and inflammation. Allosteric modulation of this receptor might be advantageous to reduce the toxicity in comparison with full agonists. Our previous results obtained with some hydroxy-chalcones, which were identified as positive allosteric modulators (PAMs) of α7 nAChR, prompted us to evaluate the potential of some structurally related naturally occurring flavonoids and curcuminoids and some synthetic curcumin analogues, with the aim of identifying new allosteric modulators of the α7 nAChR. Biological evaluation showed that phloretin, demethoxycurcumin, and bis-demethoxicurcuming behave as PAMs of α7 nAChR. In addition, some new curcumin derivatives were able to enhance the signal evoked by ACh; the activity values found for the tetrahydrocurcuminoid analog 23 were especially promising.
Comparative Study of Energy Efficient Routing Techniques in Wireless Sensor Networks
Authors: Zagrouba Rachid , Kardi Amine
Published: January 2021

This paper surveys the energy-efficient routing protocols in wireless sensor networks (WSNs). It provides a classification and comparison following a new proposed taxonomy distinguishing nine categories of protocols, namely: Latency-aware and energy-efficient routing, next-hop selection, network architecture, initiator of communication, network topology, protocol operation, delivery mode, path establishment and application type. We analyze each class, discuss its representative routing protocols (mechanisms, advantages, disadvantages…) and compare them based on different parameters under the appropriate class. Simulation results of LEACH, Mod-LEACH, iLEACH, E-DEEC, multichain-PEGASIS and M-GEAR protocols, conducted under the NS3 simulator, show that the routing task must be based on various intelligent techniques to enhance the network lifespan and guarantee better coverage of the sensing area.
Acknowledgment to Reviewers of Inorganics in 2020
Authors:   Inorganics Editorial Office
Published: January 2021

Peer review is the driving force of journal development, and reviewers are gatekeepers who ensure that Inorganics maintains its standards for the high quality of its published papers [...]
Effects of Deformed Wing Virus Infection on Expressions of Immune- and Apoptosis-Related Genes in Western Honeybees (Apis mellifera)
Authors: Mookhploy Wannapha , Krongdang Sasiprapa , Chantawannakul Panuwan
Published: January 2021

Honeybees are globally threatened by several pathogens, especially deformed wing virus (DWV), as the presence of DWV in western honeybees is indicative of colony loss. The high mortality rate is further exacerbated by the lack of effective treatment, and therefore understanding the immune and apoptosis responses could pave an avenue for the treatment method. In this study, DWV was directly injected into the white-eyed pupae stage of western honeybees (Apis mellifera). The DWV loads and selected gene responses were monitored using the real-time PCR technique. The results showed that honeybee pupae that were injected with the highest concentration of viral loads showed a significantly higher mortality rate than the control groups. Deformed wings could be observed in newly emerged adult bees when the infected bees harbored high levels of viral loads. However, the numbers of viral loads in both normal and crippled wing groups were not significantly different. DWV-injected honeybee pupae with 104 and 107 copy numbers per bee groups showed similar viral loads after 48 h until newly emerged adult bees. Levels of gene expression including immune genes (defensin, abaecin, and hymenoptaecin) and apoptosis genes (buffy, p53, Apaf1, caspase3-like, caspase8-like, and caspase9-like) were analyzed after DWV infection. The expressions of immune and apoptosis genes were significantly different in infected bees compared to those of the control groups. In the pupae stage, the immune genes were activated by injecting DWV (defensin and hymenoptaecin) or Escherichia coli (defensin, abaecin, and hymenoptaecin), a positive control. On the contrary, the expression of apoptosis-related genes (buffy, caspase3-like, caspase8-like, and caspase9-like genes) was suppressed at 96 h post-infection. In DWV-infected newly emerged adult bees, abaecin, hymenoptaecin, Apaf1, and caspase8-like genes were upregulated. However, these genes were not significantly different between the normal and crippled wing bees. Our results suggested that DWV could activate the humoral immunity in honeybees and that honeybee hosts may be able to protect themselves from the virus infection through immune responses. Apoptosis gene expressions were upregulated in newly emerged adult bees by the virus, however, they were downregulated during the initial phase of viral infection.
Critical Assessment of the Concepts and Misconceptions of the Cardiac Conduction System over the Last 100 Years: The Personal Quest of Robert H. Anderson
Authors: Sternick Eduardo Back , Sánchez-Quintana Damián
Published: January 2021

Anatomical concepts regarding the conduction system of the heart have been a matter of debate since pioneering work done at the beginning of the 20th century. Robert H. Anderson was actively involved in this field for half a century. We aimed to investigate how his own concepts evolved over time. We have assessed anatomical concepts relating to the cardiac conduction system appearing since the key contributions made in the initial decade of the 20th century, analyzing them from the perspective of Robert H. Anderson, particularly focusing on the anatomical aspects of structures such as accessory atrioventricular pathways, including the so-called Mahaim-type fibers, connections between the atrioventricular node and the atrial myocardium, and so-called “specialized” internodal atrial tracts. To accomplish this task, we have taken as our starting point the initial concepts published in the first decade of the century, along with those subsequently reported up to 1976, and assessing them in the light of our most recently published works. The concepts put forward by Robert Anderson with regard to atrioventricular nodal bypass tracts, atrioventricular nodal inputs, decrementally conducting accessory pathways, and “tracts” for internodal atrial conduction, have remained consistent along the time frame of half a century.
Chromatin Remodeler CHD8 in Autism and Brain Development
Authors: Hoffmann Anke , Spengler Dietmar
Published: January 2021

Chromodomain Helicase DNA-binding 8 (CHD8) is a high confidence risk factor for autism spectrum disorders (ASDs) and the genetic cause of a distinct neurodevelopmental syndrome with the core symptoms of autism, macrocephaly, and facial dysmorphism. The role of CHD8 is well-characterized at the structural, biochemical, and transcriptional level. By contrast, much less is understood regarding how mutations in CHD8 underpin altered brain function and mental disease. Studies on various model organisms have been proven critical to tackle this challenge. Here, we scrutinize recent advances in this field with a focus on phenotypes in transgenic animal models and highlight key findings on neurodevelopment, neuronal connectivity, neurotransmission, synaptic and homeostatic plasticity, and habituation. Against this backdrop, we further discuss how to improve future animal studies, both in terms of technical issues and with respect to the sex-specific effects of Chd8 mutations for neuronal and higher-systems level function. We also consider outstanding questions in the field including ‘humanized’ mice models, therapeutic interventions, and how the use of pluripotent stem cell-derived cerebral organoids might help to address differences in neurodevelopment trajectories between model organisms and humans.
Thermal Shock Behavior of Twill Woven Carbon Fiber Reinforced Polymer Composites
Authors: Azimpour-Shishevan Farzin , Akbulut Hamit , Mohtadi-Bonab M.A.
Published: January 2021

In the current research, the effect of cyclic temperature variation on the mechanical and thermal properties of woven carbon-fiber-reinforced polymer (CFRP) composites was investigated. To this, carbon fiber textiles in twill 2/2 pattern were used as reinforced phase in epoxy, and CFRPs were fabricated by vacuum-assisted resin-infusion molding (VARIM) method. Thermal cycling process was carried out between −40 and +120 °C for 20, 40, 60 and 80 cycles, in order to evaluate the effect of thermal cycling on mechanical and thermal properties of CFRP specimens. In this regard, tensile, bending and short beam shear (SBS) experiments were carried out, to obtain modulus of elasticity, tensile strength, flexural modulus, flexural strength and inter-laminar shear strength (ILSS) at room temperature (RT), and then thermal treated composites were compared. A dynamic mechanical analysis (DMA) test was carried out to obtain thermal properties, and viscoelastic properties, such as storage modulus (E’), loss modulus (E”) and loss factors (tan δ), were evaluated. It was observed that the characteristics of composites were affected by thermal cycling due to post-curing at a high temperature. This process worked to crosslink and improve the composite behavior or degrade it due to the different coefficients of thermal expansion (CTEs) of composite components. The response of composites to the thermal cycling process was determined by the interaction of these phenomena. Based on SEM observations, the delamination, fiber pull-out and bundle breakage were the dominant fracture modes in tensile-tested specimens.
Facile Bio-Fabrication of Ag-Cu-Co Trimetallic Nanoparticles and Its Fungicidal Activity against Candida auris
Authors: Kamli Majid Rasool , Srivastava Vartika , Hajrah Nahid H. , Sabir Jamal S. M. , Hakeem Khalid Rehman , Ahmad Aijaz , Malik Maqsood Ahmad
Published: January 2021

Candida auris is an emergent multidrug-resistant pathogen that can lead to severe bloodstream infections associated with high mortality rates, especially in hospitalized individuals suffering from serious medical problems. As Candida auris is often multidrug-resistant, there is a persistent demand for new antimycotic drugs with novel antifungal action mechanisms. Here, we reported the facile, one-pot, one-step biosynthesis of biologically active Ag-Cu-Co trimetallic nanoparticles using the aqueous extract of Salvia officinalis rich in polyphenols and flavonoids. These medicinally important phytochemicals act as a reducing agent and stabilize/capping in the nanoparticles’ fabrication process. Fourier Transform-Infrared, Scanning electron microscopy, Transmission Electron Microscopy, Energy dispersive X-Ray, X-ray powder diffraction and Thermogravimetric analysis (TGA) measurements were used to classify the as-synthesized nanoparticles. Moreover, we evaluated the antifungal mechanism of as-synthesized nanoparticles against different clinical isolates of C. auris. The minimum inhibitory concentrations and minimum fungicidal concentrations ranged from 0.39–0.78 μg/mL and 0.78–1.56 μg/mL. Cell count and viability assay further validated the fungicidal potential of Ag-Cu-Co trimetallic nanoparticles. The comprehensive analysis showed that these trimetallic nanoparticles could induce apoptosis and G2/M phase cell cycle arrest in C. auris. Furthermore, Ag-Cu-Co trimetallic nanoparticles exhibit enhanced antimicrobial properties compared to their monometallic counterparts attributed to the synergistic effect of Ag, Cu and Co present in the as-synthesized nanoparticles. Therefore, the present study suggests that the Ag-Cu-Co trimetallic nanoparticles hold the capacity to be a lead for antifungal drug development against C. auris infections.
Trade Policy Uncertainty Effects on Macro Economy and Financial Markets: An Integrated Survey and Empirical Investigation
Authors: Kyriazis Nikolaos A.
Published: January 2021

This paper conducts a review on theoretical and empirical findings on the increasingly popular measure of trade policy uncertainty (TPU) in economics and finance. Moreover, an empirical investigation takes place in order to find the impact that TPU exerts on Bitcoin market values by employing a spectrum of Generalized Autoregressive Conditional Heteroskedasticity (GARCH) specifications. Existing studies support that trade policy uncertainty leads to lower-quality and more expensive products and weak participation in international trade. Moreover, it contributes to lower democratic sentiment, hesitant internal migration and lesser socio-economic mobility and higher fluctuations in profitable assets. Moreover, our econometric findings reveal that TPU positively affects Bitcoin prices while crude oil values negatively influence this major cryptocurrency. Thereby, higher trade policy uncertainty is found to increase demand and favorite investments into risky assets in order to ameliorate the risk-return trade-off in investors’ portfolios. This study provides a compass for investing during turmoil due to trade wars and tariffs.
Urban Green Space Composition and Configuration in Functional Land Use Areas in Addis Ababa, Ethiopia, and Their Relationship with Urban Form
Authors: Woldesemayat Eyasu Markos , Genovese Paolo Vincenzo
Published: January 2021

This study aimed to assess the compositions and configurations of the urban green spaces (UGS) in urban functional land use areas in Addis Ababa, Ethiopia. The UGS data were extracted from Landsat 8 (OLI/TIRS) imagery and examined along with ancillary data. The results showed that the high-density mixed residence, medium-density mixed residence, and low-density mixed residence areas contained 16.7%, 8.7%, and 42.6% of the UGS, respectively, and together occupied 67.5% of the total UGS in the study area. Manufacturing and storage, social services, transport, administration, municipal function, and commercial areas contained 11.6%, 8.2%, 6.6%, 3.3%, 1.3%, and 1% of the UGS, respectively, together account for only 32% of the total UGS, indicating that two-third of the UGS were found in residential areas. Further, the results showed that 86.2% of individual UGS measured less than 3000 m2, while 13.8% were greater than 3000 m2, demonstrating a high level of fragmentation. The results also showed that there were strong correlations among landscape metrics, while the relationship between urban form and landscape metrics was moderate. Finally, more studies need to be conducted on the spatial pattern characteristics of UGS using very high-resolution (VHR) images. Additionally, future urban planning, design, and management need to be guided by an understanding of the composition and configuration of the UGS.
Urban Ecosystem Services: New Findings for Landscape Architects, Urban Planners, and Policymakers
Authors: Russo Alessio , Cirella Giuseppe T.
Published: January 2021

More than half of the world’s population lives in urban ecosystems [...]
Dependence of Heat Transport in Solids on Length-Scale, Pressure, and Temperature: Implications for Mechanisms and Thermodynamics
Authors: Hofmeister Anne M.
Published: January 2021

Accurate laser-flash measurements of thermal diffusivity (D) of diverse bulk solids at moderate temperature (T), with thickness L of ~0.03 to 10 mm, reveal that D(T) = D∞(T)[1 − exp(−bL)]. When L is several mm, D∞(T) = FT−G + HT, where F is constant, G is ~1 or 0, and H (for insulators) is ~0.001. The attenuation parameter b = 6.19D∞−0.477 at 298 K for electrical insulators, elements, and alloys. Dimensional analysis confirms that D → 0 as L → 0, which is consistent with heat diffusion, requiring a medium. Thermal conductivity (κ) behaves similarly, being proportional to D. Attenuation describing heat conduction signifies that light is the diffusing entity in solids. A radiative transfer model with 1 free parameter that represents a simplified absorption coefficient describes the complex form for κ(T) of solids, including its strong peak at cryogenic temperatures. Three parameters describe κ with a secondary peak and/or a high-T increase. The strong length dependence and experimental difficulties in diamond anvil studies have yielded problematic transport properties. Reliable low-pressure data on diverse thick samples reveal a new thermodynamic formula for specific heat (∂ln(cP)/∂P = −linear compressibility), which leads to ∂ln(κ)/∂P = linear compressibility + ∂lnα/∂P, where α is thermal expansivity. These formulae support that heat conduction in solids equals diffusion of light down the thermal gradient, since changing P alters the space occupied by matter, but not by light.
Evaluation Method of the Vibration Reduction Effect Considering the Real Load- and Frequency-Dependent Stiffness of Slab-Track Mats
Authors: Zhao Zeming , Wei Kai , Ding Wenhao , Cheng Fang , Wang Ping
Published: January 2021

The purpose of this research was to investigate and improve the accuracy of the existing slab-track mat (STM) specifications in the evaluation of the vibration reduction effect. The static nonlinearity and dynamic mechanical characteristics of three types of STMs were tested, and then a modified fractional derivative Poynting–Thomson (FDPT) model was used to characterize the preload and frequency dependence. A modified vehicle–floating slab track (FST) coupled dynamic model was established to analyze the actual insertion loss. The insertion loss error evaluated by the frequency-dependent tangent stiffness increased with the increase in STM nonlinearity, and the error obtained by the third preload tangent stiffness was usually greater than that of the second preload. Compared with the secant stiffness, the second preload frequency-dependent tangent stiffness was more suitable for evaluating STMs with high-static–low-dynamics (HSLD) stiffness. In order to reflect the frequency dependence effect and facilitate engineering applications, it is recommended that second preload tangent stiffness corresponding to the natural frequency of the FST be used for evaluation. Furthermore, the insertion loss of the STMs with monotonically increased stiffness decreased as the axle load increased, and the opposite was true for the STMs with monotonically decreased stiffness. The vibration isolation efficiency of the STMs with HSLD stiffness was both stable and better than that of the STMs with monotonic stiffness.
Development of Bacterial Cellulose Biocomposites Combined with Starch and Collagen and Evaluation of Their Properties
Authors: Nunes Silmar Baptista , Hodel Katharine Valéria Saraiva , Sacramento Giulia da Costa , Melo Pollyana da Silva , Pessoa Fernando Luiz Pellegrini , Barbosa Josiane Dantas Viana , Badaró Roberto , Machado Bruna Aparecida Souza
Published: January 2021

One of the major benefits of biomedicine is the use of biocomposites as wound dressings to help improve the treatment of injuries. Therefore, the main objective of this study was to develop and characterize biocomposites based on bacterial cellulose (BC) with different concentrations of collagen and starch and characterize their thermal, morphological, mechanical, physical, and barrier properties. In total, nine samples were produced with fixed amounts of glycerol and BC and variations in the amount of collagen and starch. The water activity (0.400–0.480), water solubility (12.94–69.7%), moisture (10.75–20.60%), thickness (0.04–0.11 mm), water vapor permeability (5.59–14.06 × 10−8 g·mm/m2·h·Pa), grammage (8.91–39.58 g·cm−2), opacity (8.37–36.67 Abs 600 nm·mm−1), elongation (4.81–169.54%), and tensile strength (0.99–16.32 MPa) were evaluated and defined. In addition, scanning electron microscopy showed that adding biopolymers in the cellulose matrix made the surface compact, which also influenced the visual appearance. Thus, the performance of the biocomposites was directly influenced by their composition. The performance of the different samples obtained resulted in them having different potentials for application considering the injury type. This provides a solution for the ineffectiveness of traditional dressings, which is one of the great problems of the biomedical sector.
Determination of the Shear Modulus of Pine Wood with the Arcan Test and Digital Image Correlation
Authors: Bilko Piotr , Skoratko Aneta , Rutkiewicz Andrzej , Małyszko Leszek
Published: January 2021

Arcan shear tests with digital image correlation were used to evaluate the shear modulus and shear stress–strain diagrams in the plane defined by two principal axes of the material orthotropy. Two different orientation of the grain direction as compared to the direction of the shear force in specimens were considered: perpendicular and parallel shear. Two different ways were used to obtain the elastic properties based on the digital image correlation (DIC) results from the full-field measurement and from the virtual strain gauges with the linear strains: perpendicular to each other and directed at the angle of π/4 to the shearing load. In addition, the own continuum structural model for the failure analysis in the experimental tests was used. Constitutive relationships of the model were established in the framework of the mathematical multi-surface elastoplasticity for the plane stress state. The numerical simulations done by the finite element program after implementation of the model demonstrated the failure mechanisms from the experimental tests.
Subordination Properties of Meromorphic Kummer Function Correlated with Hurwitz–Lerch Zeta-Function
Authors: Ghanim Firas , Al-Shaqsi Khalifa , Darus Maslina , Al-Janaby Hiba Fawzi
Published: January 2021

Recently, Special Function Theory (SPFT) and Operator Theory (OPT) have acquired a lot of concern due to their considerable applications in disciplines of pure and applied mathematics. The Hurwitz-Lerch Zeta type functions, as a part of Special Function Theory (SPFT), are significant in developing and providing further new studies. In complex domain, the convolution tool is a salutary technique for systematic analytical characterization of geometric functions. The analytic functions in the punctured unit disk are the so-called meromorphic functions. In this present analysis, a new convolution complex operator defined on meromorphic functions related with the Hurwitz-Lerch Zeta type functions and Kummer functions is considered. Certain sufficient stipulations are stated for several formulas of this defining operator to attain subordination. Indeed, these outcomes are an extension of known outcomes of starlikeness, convexity, and close to convexity.