bluuebunny/crossref_metadata_2025 huggingface dataset:
Coelonin, an Anti-Inflammation Active Component of Bletilla striata and Its Potential Mechanism
Authors: Jiang Fusheng , Li Meiya , Wang Hongye , Ding Bin , Zhang Chunchun , Ding Zhishan , Yu Xiaobo , Lv Guiyuan
Published: September 2019

Ethanol extract of Bletilla striata has remarkable anti-inflammatory and anti-pulmonary fibrosis activities in the rat silicosis model. However, its active substances and molecular mechanism are still unclear. To uncover the active ingredients and potential molecular mechanism of the Bletilla striata extract, the lipopolysaccharide (LPS)-induced macrophage inflammation model and phospho antibody array were used. Coelonin, a dihydrophenanthrene compound was isolated and identified. It significantly inhibited LPS-induced interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) expression at 2.5 μg/mL. The microarray data indicate that the phosphorylation levels of 32 proteins in the coelonin pre-treated group were significantly down-regulated. In particular, the phosphorylation levels of the key inflammatory regulators factor nuclear factor-kappa B (NF-κB) were significantly reduced, and the negative regulator phosphatase and tensin homologue on chromosome ten (PTEN) was reduced. Moreover, the phosphorylation level of cyclin dependent kinase inhibitor 1B (p27Kip1), another downstream molecule regulated by PTEN was also reduced significantly. Western blot and confocal microscopy results confirmed that coelonin inhibited LPS-induced PTEN phosphorylation in a dose-dependent manner, then inhibited NF-κB activation and p27Kip1 degradation by regulating the phosphatidylinositol-3-kinases/ v-akt murine thymoma viral oncogene homolog (PI3K/AKT) pathway negatively. However, PTEN inhibitor co-treatment analysis indicated that the inhibition of IL-1β, IL-6 and TNF-α expression by coelonin was independent of PTEN, whereas the inhibition of p27Kip1 degradation resulted in cell-cycle arrest in the G1 phase, which was dependent on PTEN. The anti-inflammatory activity of coelonin in vivo, which is one of the main active ingredients of Bletilla striata, deserves further study.
FMK, an Inhibitor of p90RSK, Inhibits High Glucose-Induced TXNIP Expression via Regulation of ChREBP in Pancreatic β Cells
Authors: Han Jung-Hwa , Kim Suji , Kim Sujin , Lee Heejung , Park So-Young , Woo Chang-Hoon
Published: September 2019

Hyperglycemia is the major characteristic of diabetes mellitus, and a chronically high glucose (HG) level causes β-cell glucolipotoxicity, which is characterized by lipid accumulation, impaired β-cell function, and apoptosis. TXNIP (Thioredoxin-interacting protein) is a key mediator of diabetic β-cell apoptosis and dysfunction in diabetes, and thus, its regulation represents a therapeutic target. Recent studies have reported that p90RSK is implicated in the pathogenesis of diabetic cardiomyopathy and nephropathy. In this study, we used FMK (a p90RSK inhibitor) to determine whether inhibition of p90RSK protects β-cells from chronic HG-induced TXNIP expression and to investigate the molecular mechanisms underlying the effect of FMK on its expression. In INS-1 pancreatic β-cells, HG-induced β-cell dysfunction, apoptosis, and ROS generation were significantly diminished by FMK. In contrast BI-D1870 (another p90RSK inhibitor) did not attenuate HG-induced TXNIP promoter activity or TXNIP expression. In addition, HG-induced nuclear translocation of ChREBP and its transcriptional target molecules were found to be regulated by FMK. These results demonstrate that HG-induced pancreatic β-cell dysfunction resulting in HG conditions is associated with TXNIP expression, and that FMK is responsible for HG-stimulated TXNIP gene expression by inactivating the regulation of ChREBP in pancreatic β-cells. Taken together, these findings suggest FMK may protect against HG-induced β-cell dysfunction and TXNIP expression by ChREBP regulation in pancreatic β-cells, and that FMK is a potential therapeutic reagent for the drug development of diabetes and its complications.
The Insulin/IGF System in Mammalian Sexual Development and Reproduction
Authors: Neirijnck Yasmine , Papaioannou Marilena D. , Nef Serge
Published: September 2019

Persistent research over the past few decades has clearly established that the insulin-like family of growth factors, which is composed of insulin and insulin-like growth factors 1 (IGF1) and 2 (IGF2), plays essential roles in sexual development and reproduction of both males and females. Within the male and female reproductive organs, ligands of the family act in an autocrine/paracrine manner, in order to guide different aspects of gonadogenesis, sex determination, sex-specific development or reproductive performance. Although our knowledge has greatly improved over the last years, there are still several facets that remain to be deciphered. In this review, we first briefly outline the principles of sexual development and insulin/IGF signaling, and then present our current knowledge, both in rodents and humans, about the involvement of insulin/IGFs in sexual development and reproductive functions. We conclude by highlighting some interesting remarks and delineating certain unanswered questions that need to be addressed in future studies.
Constructing and Visualizing High-Quality Classifier Decision Boundary Maps
Authors: Rodrigues Francisco C. M. , Espadoto Mateus , Hirata Roberto , Telea Alexandru C.
Published: September 2019

Visualizing decision boundaries of machine learning classifiers can help in classifier design, testing and fine-tuning. Decision maps are visualization techniques that overcome the key sparsity-related limitation of scatterplots for this task. To increase the trustworthiness of decision map use, we perform an extensive evaluation considering the dimensionality-reduction (DR) projection techniques underlying decision map construction. We extend the visual accuracy of decision maps by proposing additional techniques to suppress errors caused by projection distortions. Additionally, we propose ways to estimate and visually encode the distance-to-decision-boundary in decision maps, thereby enriching the conveyed information. We demonstrate our improvements and the insights that decision maps convey on several real-world datasets.
The Changing Landscape of Acute Kidney Injury in Pregnancy from an Obstetrics Perspective
Authors: Vinturache Angela , Popoola Joyce , Watt-Coote Ingrid
Published: September 2019

Pregnancy-related acute kidney injury (PR-AKI) is a heterogeneous disorder with multiple aetiologies that can occur at any time throughout pregnancy and the post-partum period. PR-AKI is an important obstetric complication that is associated with significant maternal and foetal morbidity and mortality. Although there has been an overall decline in the incidence of PR-AKI worldwide, a recent shift in the occurrence of this disease has been reported. Following improvements in obstetric care, PR-AKI incidence has been reduced in developing countries, whereas an increase in PR-AKI incidence has been reported in developed countries. Awareness of the physiological adaptations of the renal system is essential for the diagnosis and management of kidney impairment in pregnancy. In this review we scrutinize the factors that have contributed to the changing epidemiology of PR-AKI and discuss challenges in the diagnosis and management of acute kidney injury (AKI) in pregnancy from an obstetrics perspective. Thereafter we provide brief discussions on the diagnostic approach of certain PR-AKI aetiologies and summarize key therapeutic measures.
Tracking Photodynamic- and Chemotherapy-Induced Redox-State Perturbations in 3D Culture Models of Pancreatic Cancer: A Tool for Identifying Therapy-Induced Metabolic Changes
Authors: Broekgaarden Mans , Bulin Anne-Laure , Frederick Jane , Mai Zhiming , Hasan Tayyaba
Published: September 2019

The metabolic plasticity of cancer cells is considered a highly advantageous phenotype that is crucial for disease progression and acquisition of treatment resistance. A better understanding of cancer metabolism and its adaptability after treatments is vital to develop more effective therapies. To screen novel therapies and combination regimens, three-dimensional (3D) culture models of cancers are attractive platforms as they recapitulate key features of cancer. By applying non-perturbative intensity-based redox imaging combined with high-throughput image analysis, we demonstrated metabolic heterogeneity in various 3D culture models of pancreatic cancer. Photodynamic therapy and oxaliplatin chemotherapy, two cancer treatments with relevance to pancreatic cancer, induced perturbations in redox state in 3D microtumor cultures of pancreatic cancer. In an orthotopic mouse model of pancreatic cancer, a similar disruption in redox homeostasis was observed on ex vivo slices following photodynamic therapy in vivo. Taken together, redox imaging on cancer tissues combined with high-throughput analysis can elucidate dynamic spatiotemporal changes in metabolism following treatment, which will benefit the design of new metabolism-targeted therapeutic approaches.
Tachycardiomyopathy in Patients without Underlying Structural Heart Disease
Authors: Stronati Giulia , Guerra Federico , Urbinati Alessia , Ciliberti Giuseppe , Cipolletta Laura , Capucci Alessandro
Published: September 2019

Tachycardiomyopathy (TCM) is an underestimated cause of reversible left ventricle dysfunction. The aim of this study was to identify the predictors of recurrence and incidence of major cardiovascular events in TCM patients without underlying structural heart disease (pure TCM). The prospective, observational study enrolled all consecutive pure TCM patients. The diagnosis was suspected in patients admitted for heart failure (HF) with a reduced ejection fraction and concomitant persistent arrhythmia. Pure TCM was confirmed after the clinical and echocardiographic recovery during follow-up. From 107 pure TCM patients (9% of all HF admission, the median follow-up 22.6 months), 17 recurred, 51 were hospitalized for cardiovascular reasons, two suffered from thromboembolic events and one died. The diagnosis of obstructive sleep apnoea syndrome (OSAS, hazard ratio (HR) 5.44), brain natriuretic peptide on admission (HR 1.01 for each pg/mL) and the heart rate at discharge (HR 1.05 for each bpm) were all independent predictors of TCM recurrence. The left ventricular ejection fraction at discharge (HR 0.96 for each%) and the heart rate at discharge (HR 1.02 for each bpm) resulted as independent predictors of cardiovascular-related hospitalization. Pure TCM is more common than previously thought and associated with a good long-term survival but recurrences and hospitalizations are frequent. Reversing OSAS and controlling the heart rate could prevent TCM-related complications.
The Role of Mesenchymal Stem Cells in Atherosclerosis: Prospects for Therapy via the Modulation of Inflammatory Milieu
Authors: Mahdavi Gorabi Armita , Banach Maciej , Reiner Željko , Pirro Matteo , Hajighasemi Saeideh , Johnston Thomas P. , Sahebkar Amirhossein
Published: September 2019

Atherosclerosis is a chronic, inflammatory disease that mainly affects the arterial intima. The disease is more prevalent in middle-age and older individuals with one or more cardiovascular risk factors, including dyslipidemia, hypertension, diabetes, smoking, obesity, and others. The beginning and development of atherosclerosis has been associated with several immune components, including infiltration of inflammatory cells, monocyte/macrophage-derived foam cells, and inflammatory cytokines and chemokines. Mesenchymal stem cells (MSCs) originate from several tissue sources of the body and have self-renewal and multipotent differentiation characteristics. They also have immunomodulatory and anti-inflammatory properties. Recently, it was shown that MSCs have a regulatory role in plasma lipid levels. In addition, MSCs have shown to have promising potential in terms of treatment strategies for several diseases, including those with an inflammatory component. In this regard, transplantation of MSCs to patients with atherosclerosis has been proposed as a novel strategy in the treatment of this disease. In this review, we summarize the current advancements regarding MSCs for the treatment of atherosclerosis.
MRI-Tracking of Dental Pulp Stem Cells In Vitro and In Vivo Using Dextran-Coated Superparamagnetic Iron Oxide Nanoparticles
Authors: Zare Shahrokh , Mehrabani Davood , Jalli Reza , Saeedi Moghadam Mahdi , Manafi Navid , Mehrabani Golshid , Jamhiri Iman , Ahadian Samad
Published: September 2019

The aim of this study was to track dental pulp stem cells (DPSCs) labeled with dextran-coated superparamagnetic iron oxide nanoparticles (SPIONs) using magnetic resonance imaging (MRI). Dental pulp was isolated from male Sprague Dawley rats and cultured in Dulbecco’s modified Eagle’s medium F12 (DMEM-F12) and 10% fetal bovine serum. Effects of SPIONs on morphology, viability, apoptosis, stemness, and osteogenic and adipogenic differentiation of DPSCs were assessed. Prussian blue staining and MRI were conducted to determine in vitro efficiency of SPIONs uptake by the cells. Both non-labeled and labeled DPSCs were adherent to culture plates and showed spindle-shape morphologies, respectively. They were positive for osteogenic and adipogenic induction and expression of cluster of differentiation (CD) 73 and CD90 biomarkers, but negative for expression of CD34 and CD45 biomarkers. The SPIONs were non-toxic and did not induce apoptosis in doses less than 25 mg/mL. Internalization of the SPIONs within the DPSCs was confirmed by Prussian blue staining and MRI. Our findings revealed that the MRI-based method could successfully monitor DPSCs labeled with dextran-coated SPIONs without any significant effect on osteogenic and adipogenic differentiation, viability, and stemness of DPSCs. We provided the in vitro evidence supporting the feasibility of an MRI-based method to monitor DPSCs labeled with SPIONs without any significant reduction in viability, proliferation, and differentiation properties of labeled cells, showing that internalization of SPIONs within DPSCs were not toxic at doses less than 25 mg/mL. In general, the SPION labeling does not seem to impair cell survival or differentiation. SPIONs are biocompatible, easily available, and cost effective, opening a new avenue in stem cell labeling in regenerative medicine.
Study of an Additional Layer of Cement Mantle Hip Joints for Reducing Cracks
Authors: Jamari J. , Saputra Eko , Anwar Iwan Budiwan , van der Heide Emile
Published: September 2019

Failure of the cement mantle in total hip arthroplasty is not a simple phenomenon. Cracking, which can be caused by crack initiation and repeated loading, can cause loosening of the acetabular liner component. A previous study showed that addition of a metal layer between the liner and acetabular could reduce the stress at the contact surface of the cement mantle. This study elaborates on the performance of the additional layer. Several material properties of the layer were simulated using finite element analysis for maximum performance. A static contact analysis was used to simulate the stresses at the contact surface of the cement mantle. The results show that an additional layer of cobalt chrome produced the best performance.
A Comparative Study of Helical and Cross-Wedge Rolling Processes for Producing Ball Studs
Authors: Bulzak Tomasz , Tomczak Janusz , Pater Zbigniew , Majerski Krzysztof
Published: September 2019

This paper presents two rolling technologies: cross-wedge rolling (CWR) and helical-wedge rolling (HWR). The two rolling processes were compared using the example of rolling a ball stud forging. The technologies were modeled in the finite element model (FEM) environment. Calculations were performed to obtain distributions of strain and the Cockcroft–Latham damage criterion. The investigated processes were also performed under real-life conditions. The results of the experiments were used to compare the force and energy parameters of the rolling technologies. Tests were also carried out to investigate the microstructure of the studs and a grain size after rolling. The state of the macrostructure, i.e., the grain flow lines, was also compared. The experiments showed that HWR was a more energy-efficient process.
Self-Gathering Effect of the Hydrogen Diffusion in Welding Induced by the Solid-State Phase Transformation
Authors: Xiong Zhiliang , Zheng Wenjian , Tang Liping , Yang Jianguo
Published: September 2019

The hydrogen diffusion in welding was investigated by using thermal-mechanical-hydrogen diffusion sequential coupled procedures based on finite element method. A self-gathering effect induced by the solid-state phase transformation was discovered. Because of the self-gathering effect, the hydrogen concentration in weld metal was accumulated to a peak value which can be larger than the initial hydrogen concentration in molten pool, and subsequently the hydrogen concentration in heat affect zone was redistributed. In multi-pass welding, the gathered effect not only happened inside a weld pass, but also in the inter-pass, which further increased the sensitivity of the hydrogen-assisted cold cracking. Controlling should be adopted to restrain the hydrogen accumulation. Welding stress evolution during the solid-state phase transformation process had limited effect on the hydrogen diffusion.
Structured Porous Material Design for Passive Flow and Noise Control of Cylinders in Uniform Flow
Authors: Arcondoulis Elias J. G. , Liu Yu , Li Zhiyong , Yang Yannian , Wang Yong
Published: September 2019

Cylindrical bodies in uniform flows can be coated with a porous medium as a passive flow and noise control method in an effort to reduce the acoustic effects of vortex shedding. To date, the employed open-cell porous materials typically possess a randomized internal structure. This paper presents the design and validation of a novel 3-D printed structured porous coated cylinder that has significant flexibility, in that the porosity and pores per inch of the porous coating can be modified independently and relatively easily. The performance of the structured porous coating design is compared against porous polyurethane and metal foam with the same coating dimensions and similar pores per inch and porosity via an experimental acoustic investigation, revealing strong similarity in the passive noise control performance of each material type. A numerical comparison illustrates the similarities of the wake structure of the 3-D printed porous coated cylinder to an equivalent Darcy–Forchheimer model simulation that represents a randomized internal porous structure. The performance similarities of these different porous material types indicate that a structured porous geometry can be used to understand the internal flow behavior of the porous medium responsible for reducing the cylinder vortex shedding tone that is otherwise extremely difficult or impossible with typical randomized porous structures. Moreover, significant potential exists for the porous structure to be further optimized or smartly tailored by architectural design for different control purposes, coating geometries and dimensions, and working conditions.
Ligand-Modified Boron-Doped Diamond Surface: DFT Insights into the Electronic Properties of Biofunctionalization
Authors: Dec Bartłomiej , Sobaszek Michał , Jaramillo-Botero Andrés , Goddard William Andrew , Bogdanowicz Robert
Published: September 2019

With the increasing power of computation systems, theoretical calculations provide a means for quick determination of material properties, laying out a research plan, and lowering material development costs. One of the most common is Density Functional Theory (DFT), which allows us to simulate the structure of chemical molecules or crystals and their interaction. In developing a new generation of biosensors, understanding the nature of functional linkers, antibodies, and ligands become essential. In this study, we used DFT to model a bulk boron-doped diamond slab, modified by a functional linker and a surrogate proteins ligand. DTF calculations enable the prediction of electronic transport properties in an electrochemical sensor setup, composed of a boron-doped diamond electrode functionalized by 4-amino benzoic acids and a target surrogated protein-ligand for influenza. Electron conduction pathways and other signatures associated with the detection and measurement of the target analyte are revealed.
Interpreting the Terahertz Spectrum of Complex Materials: The Unique Contribution of the Bayesian Analysis
Authors: De Francesco Alessio , Scaccia Luisa , Maccarini Marco , Formisano Ferdinando , Guarini Eleonora , Bafile Ubaldo , Cunsolo Alessandro
Published: September 2019

In the last few decades, experimental studies of the terahertz spectrum of density fluctuations have considerably improved our knowledge of the mesoscopic dynamics of disordered materials, which also have imposed new demands on the data modelling and interpretation. Indeed, lineshape analyses are no longer limited to the phenomenological observation of inelastic features, as in the pioneering stage of Neutron or X-ray spectroscopy, rather aiming at the extraction from their shape of physically relevant quantities, as sound velocity and damping, relaxation times, or other transport coefficients. In this effort, researchers need to face both inherent and practical obstacles, respectively stemming from the highly damped nature of terahertz modes and the limited energy resolution, accessible kinematic region and statistical accuracy of the typical experimental outcome. To properly address these challenges, a global reconsideration of the lineshape modelling and the enforcement of evidence-based probabilistic inference is becoming crucial. Particularly compelling is the possibility of implementing Bayesian inference methods, which we illustrated here through an in-depth discussion of some results recently obtained in the analysis of Neutron and X-ray scattering results.
Vascular Dysfunction among Malaysian Men with Increased BMI: An Indication of Synergistic Effect of Free Testosterone and Inflammation
Authors: Aminuddin Amilia , Salamt Norizam , Ahmad Fuad Ahmad Faiz , Chin Kok-Yong , Ugusman Azizah , Soelaiman Ima Nirwana , Wan Ngah Wan Zurinah
Published: September 2019

Background and objectives: Obesity is associated with poor vascular function and may lead to future cardiovascular disease (CVD). Obesity is also related to increased inflammation and a low testosterone level. This study was conducted to determine the relationship between inflammation, testosterone level, and vascular function among subjects with an increased body mass index (BMI) and to determine whether both low testosterone and high inflammation have synergistic effects towards vascular dysfunction. Materials and Methods: A total of 303 men aged 40–80 years were recruited from Klang Valley, Malaysia. Their height, weight, blood pressure (BP), lipid, blood glucose level, total testosterone (TT), free testosterone (FT), and C-reactive protein (CRP) were measured. The carotid femoral pulse wave velocity (PWVCF) and augmentation index (AI) were also recorded as markers of vascular function. Results: The mean age of all the subjects was 54.46 ± 9.77 years. Subjects were divided into a low/normal body mass index (BMI) group (BMI < 25 kg/m2; NG, n = 154) and high BMI group (BMI ≥ 25 kg/m2; OG, n = 149). The mean BMI for NG was 22.20 ± 1.94 kg/m2 while for OG was 28.87 ± 3.24 kg/m2 (p < 0.01). The level of TT (OG = 21.13 ± 6.44 versus NG = 16.18 ± 6.16 nmol/L, p < 0.01) and FT (OG = 0.34 ± 0.12 versus NG = 0.39 ± 0.11 nmol/L, p < 0.01) were reduced while the level of CRP [OG = 1.05 (2.80) versus NG = 0.50 (1.50) mmol/L, p = 0.01] was increased in OG compared to NG. PWVCF (OG = 8.55 ± 1.34 versus NG = 8.52 ± 1.42 m/s, p = 0.02) and AI (OG = 16.91% ± 6.00% versus 15.88% ± 5.58%, p < 0.01) were significantly increased in OG after adjustment for other CVD risk factors. The subjects that had both a low FT and an increased CRP had higher AI when compared to those with a high CRP and high FT (p < 0.01). Conclusions: The increased BMI was associated with vascular dysfunction, mediated by a low testosterone level and increased inflammation. Furthermore, having both conditions concurrently lead to higher vascular dysfunction. Weight loss, testosterone supplementation, and the anti-inflammatory agent may be beneficial for men to prevent vascular dysfunction.
Effect of Gallium Content and Heat Treatment on the Microstructure and Corrosion Rate of Magnesium Binary Alloys
Authors: Hernández-Cortés Anabel A. , Escobedo-Bocardo José C. , Cortés-Hernández Dora A. , Almanza-Robles José M.
Published: September 2019

The microstructure and corrosion rate of as-cast and heat-treated binary Mg-Ga alloys with gallium content ranging from 0.375 to 1.5 wt. % were investigated. The corrosion rate was determined by the weight loss method using a simulated body fluid (SBF). The microstructure of the as-cast alloys showed an α-Mg matrix of dendritic morphology with intermetallic compounds Mg5Ga2 located mainly at the interdendritic regions. The fraction and size of the Mg5Ga2 particles increased with the amount of Ga in the alloy. The grain size decreased as the Ga content was increased. The products formed on the surface of the Mg-Ga alloys after immersion in SBF were MgO, Mg(OH)2, and calcium phosphates. The corrosion rate of the as-cast alloys was dependent on the Ga content. At concentrations lower than 1 wt. % the corrosion rate was similar to that of pure Mg (0.65 mm/year). However, Ga additions higher than 1 wt. % worsened the corrosion resistance. After heat treatment, the corrosion rate of Mg-Ga alloys decreased, and in the case of the alloys with Ga concentrations lower than 1 wt. %, corrosion rate was lower than that of pure Mg. Corrosion of these alloys after heat treatment was uniform.
Acquiring High-Quality Oil Casing Steel 26CrMoVTiB under Optimal Continuous Casting Process Conditions
Authors: Zhai Yingying , Pan Kefeng , Wu Dapeng
Published: September 2019

While the solidification macrostructure of continuous cast billets is an important factor influencing the final performance and rolling yield of oil casing steel, the continuous casting process parameters have a direct influence on the solidification structure. This study simulated the solidification process of the continuous casting round billets of oil casing steel using a cellular automaton–finite element (CAFE) model. According to the simulation results, at a superheat degree of 20–35 K, a casting speed of 1.9–2.1 m/min, and a secondary cooling specific water flow of 0.34–0.45 L/Kg, the solidification structure had a relatively high equiaxed crystal ratio and small average grain radius. Guided by the simulation results, this paper establishes optimal process schemes for producing 26CrMoVTiB steel round billets, comparatively analyzes the equiaxed crystal ratio and central shrinkage of round billets produced according to these schemes, and defines the optimal continuous casting process conditions, which are: superheat degree = 25 K, casting speed = 2.1 m/min, and specific water flow = 0.35 L/Kg. When adopting these process parameters, the 26CrMoVTiB steel round billets demonstrate a tiny central shrinkage and an equiaxed crystal ratio of 45.2%.
Effects of Solid Content and Substrate Concentration on Bioleaching of Heavy Metals from Sewage Sludge Using Aspergillus niger
Authors: Chen Shen-Yi , Wang Sheng-Ying
Published: September 2019

The presence of heavy metals in sewage sludge not only affects the performance of sludge anaerobic digestion process but also restricts the land application of treated sewage sludge. Therefore, a fungi-mediated bioleaching process for simultaneous metal leaching and sludge digestion by Aspergillus niger was developed to treat the sewage sludge containing heavy metals in this study. The effects of two important parameters, sludge solid content and substrate (sucrose) concentration, on the performance of fungal bioleaching were investigated in this study. The results showed that the rate of pH reduction increased with increasing sludge solid contents and sucrose concentrations. In this study, the efficiency of metal removal decreases in the order of Mn > Zn > Ni > Pb. The efficiencies of metal leaching and solid degradation (SS and VSS) were found to be decreased with an increase of sludge solid content and a decrease of sucrose concentration. At 2 days of reaction time, the maximum efficiency of metal solubilization was 95, 56, 21 and 13% for Mn, Zn, Ni and Pb, respectively.
A Resonant Z-Axis Aluminum Nitride Thin-Film Piezoelectric MEMS Accelerometer
Authors: Yang Jian , Zhang Meng , He Yurong , Su Yan , Han Guowei , Si Chaowei , Ning Jin , Yang Fuhua , Wang Xiaodong
Published: September 2019

In this paper, we report a novel aluminum nitride (AlN) thin-film piezoelectric resonant accelerometer. Different from the ordinary MEMS (micro-electro-mechanical systems) resonant accelerometers, the entire structure of the accelerometer, including the mass and the springs, is excited to resonate in-plane, and the resonance frequency is sensitive to the out-plane acceleration. The structure is centrosymmetrical with serpentine electrodes laid on supporting beams for driving and sensing. The stiffness of the supporting beams changes when an out-plane inertial force is applied on the structure. Therefore, the resonance frequency of the accelerometer will also change under the inertial force. The working principle is analyzed and the properties are simulated in the paper. The proposed AlN accelerometer is fabricated by the MEMS technology, and the structure is released by an ICP isotropic etching. The resonance frequency is 24.66 kHz at a static state. The quality factor is 1868. The relative sensitivity of this accelerometer, defined as the shift in the resonance frequency per gravity unit (1 g = 9.8 m/s2) is 346 ppm/g. The linearity of the accelerometer is 0.9988. The temperature coefficient of frequency (TCF) of this accelerometer is −2.628 Hz/°C (i.e., −106 ppm/°C), tested from −40 °C to 85 °C.
Complexes of Glycolic Acid with Nitrogen Isolated in Argon Matrices. II. Vibrational Overtone Excitations
Authors: Kosendiak Iwona , Ahokas Jussi M.E. , Krupa Justyna , Lundell Jan , Wierzejewska Maria
Published: September 2019

Structural changes of glycolic acid (GA) complex with nitrogen induced by selective overtone excitation of the νOH mode were followed in argon matrices using FTIR spectroscopy. For the most stable SSC1 complex present in different trapping sites directly upon deposition site, selective changes in the νOH region were achieved upon near-infrared irradiation. Simultaneously, new conformers of the GA…N2 complex were formed, giving rise to several sets of bands in the νOH and νC=O regions of the spectra. Both position and intensity of new absorptions appeared to be highly sensitive on the wavelength of radiation used, as well as on the annealing of the matrix. Based on theoretical calculations at different levels of theory, an assignment of the observed bands is proposed and discussed.
Complexes of Glycolic Acid with Nitrogen Isolated in Argon Matrices. I. Structures and Thermal Effects
Authors: Kosendiak Iwona , Ahokas Jussi M.E. , Krupa Justyna , Lundell Jan , Wierzejewska Maria
Published: September 2019

Molecular complexes between glycolic acid and nitrogen were studied in a low-temperature argon matrix with FTIR spectroscopy, and supported by MP2 and BLYPD3 calculations. The calculations indicate 11 and 10 stable complex structures at the MP2 and BLYPD3 levels of theories, respectively. However, only one hydrogen-bonded complex structure involving the most stable SSC conformer of glycolic acid was found experimentally, where the nitrogen molecule is bound with the carboxylic OH group of the SSC conformer. The complex shows a rich site structure variation upon deposition of the matrix in different temperatures and upon annealing experiments, which provide interesting prospects for site-selective chemistry.
Near-Infrared Hyperspectral Imaging Combined with Deep Learning to Identify Cotton Seed Varieties
Authors: Zhu Susu , Zhou Lei , Gao Pan , Bao Yidan , He Yong , Feng Lei
Published: September 2019

Cotton seed purity is a critical factor influencing the cotton yield. In this study, near-infrared hyperspectral imaging was used to identify seven varieties of cotton seeds. Score images formed by pixel-wise principal component analysis (PCA) showed that there were differences among different varieties of cotton seeds. Effective wavelengths were selected according to PCA loadings. A self-design convolution neural network (CNN) and a Residual Network (ResNet) were used to establish classification models. Partial least squares discriminant analysis (PLS-DA), logistic regression (LR) and support vector machine (SVM) were used as direct classifiers based on full spectra and effective wavelengths for comparison. Furthermore, PLS-DA, LR and SVM models were used for cotton seeds classification based on deep features extracted by self-design CNN and ResNet models. LR and PLS-DA models using deep features as input performed slightly better than those using full spectra and effective wavelengths directly. Self-design CNN based models performed slightly better than ResNet based models. Classification models using full spectra performed better than those using effective wavelengths, with classification accuracy of calibration, validation and prediction sets all over 80% for most models. The overall results illustrated that near-infrared hyperspectral imaging with deep learning was feasible to identify cotton seed varieties.
Chemical Composition of Two Different Lavender Essential Oils and Their Effect on Facial Skin Microbiota
Authors: Białoń Marietta , Krzyśko-Łupicka Teresa , Nowakowska-Bogdan Ewa , Wieczorek Piotr P.
Published: September 2019

Lavender oil is one of the most valuable aromatherapy oils, its anti-bacterial and anti-fungal activities can be explained by main components such as linalool, linalyl acetate, lavandulol, geraniol, or eucalyptol. The aim of the study was to assess the anti-microbial effects of two different lavender oils on a mixed microbiota from facial skin. The commercial lavender oil and essential lavender oil from the Crimean Peninsula, whose chemical composition and activity are yet to be published, were used. Both oils were analysed by gas chromatography coupled to mass spectrometry. The composition and properties of studied oils were significantly different. The commercial ETJA lavender oil contained 10% more linalool and linalyl acetate than the Crimean lavender oil. Both oils also had different effects on the mixed facial skin microbiota. The Gram-positive bacilli were more sensitive to ETJA lavender oil, and Gram-negative bacilli were more sensitive to Crimean lavender oil. However, neither of the tested oils inhibited the growth of Gram-positive cocci. The tested lavender oils decreased the cell number of the mixed microbiota from facial skin, but ETJA oil showed higher efficiency, probably because it contains higher concentrations of monoterpenoids and monoterpenes than Crimean lavender oil does.
Effects of High Pressure Modification on Conformation and Digestibility Properties of Oyster Protein
Authors: Wang Runfang , Jiang Suisui , Li Yujin , Xu Yunsheng , Zhang Tietao , Zhang Fan , Feng Xue , Zhao Yuanhui , Zeng Mingyong
Published: September 2019

To expand the utilization of oyster protein (OP), the effects of high pressure (100 to 500 MPa) on chemical forces, structure, microstructure, and digestibility properties were investigated. High pressure (HP) treatment enhanced the electrostatic repulsion (from −13.3Control to −27.8HP200 mV) between protein molecules and avoided or retarded the formation of protein aggregates. In addition, the HP treated samples showed uniform distribution and small particle size. The changes in electrostatic interaction and particle size contributed to the improvement of solubility (from 10.53%Control to 19.92%HP500 at pH 7). The stretching and unfolding of protein were modified by HP treatment, and some internal hydrophobic groups and -SH groups were exposed. HP treatment modified the secondary structure of OP. The treated samples contained less α-helix and β-sheet structures, whereas the proportions of β-sheet and random coil structures were increased. The treated samples have high digestibility in the stomach (from 26.3%Control to 39.5%HP500) and in the total digestive process (from 62.1%Control to 83.7%HP500). In addition, the total digestive production showed higher percentages of small peptides (<1 kDa) after HP treatment. The protein solubility and digestibility were increased after HP treatment, and high solubility and high digestibility might increase the chance that OP become a kind of protein supplement.
CLEFMA Activates the Extrinsic and Intrinsic Apoptotic Processes through JNK1/2 and p38 Pathways in Human Osteosarcoma Cells
Authors: Yang Jia-Sin , Lin Renn-Chia , Hsieh Yi-Hsien , Wu Heng-Hsiung , Li Geng-Chung , Lin Ya-Chiu , Yang Shun-Fa , Lu Ko-Hsiu
Published: September 2019

Due to the poor prognosis of metastatic osteosarcoma, chemotherapy is usually employed in the adjuvant situation to improve the prognosis and the chances of long-term survival. 4-[3,5-Bis(2-chlorobenzylidene)-4-oxo-piperidine-1-yl]-4-oxo-2-butenoic acid (CLEFMA) is a synthetic analog of curcumin and possesses anti-inflammatory and anticancer properties. To further obtain information regarding the apoptotic pathway induced by CLEFMA in osteosarcoma cells, microculture tetrazolium assay, annexin V-FITC/PI apoptosis staining assay, human apoptosis array, and Western blotting were employed. CLEFMA dose-dependently decreased the cell viabilities of human osteosarcoma U2OS and HOS cells and significantly induced apoptosis in human osteosarcoma cells. In addition to the effector caspase 3, CLEFMA significantly activated both extrinsic caspase 8 and intrinsic caspase 9 initiators. Moreover, CLEFMA increased the phosphorylation of extracellular signal-regulated protein kinases (ERK)1/2, c-Jun N-terminal kinases (JNK)1/2 and p38. Using inhibitors of JNK (JNK-in-8) and p38 (SB203580), CLEFMA’s increases of cleaved caspases 3, 8, and 9 could be expectedly suppressed, but they could not be affected by co-treatment with the ERK inhibitor (U0126). Conclusively, CLEFMA activates both extrinsic and intrinsic apoptotic pathways in human osteosarcoma cells through JNK and p38 signaling. These findings contribute to a better understanding of the mechanisms responsible for CLEFMA’s apoptotic effects on human osteosarcoma cells.
Complement Activation-Related Pathophysiological Changes in Anesthetized Rats: Activator-Dependent Variations of Symptoms and Mediators of Pseudoallergy
Authors: Dézsi László , Mészáros Tamás , Őrfi Erik , Fülöp Tamás , Hennies Mark , Rosivall László , Hamar Péter , Szebeni János , Szénási Gábor
Published: September 2019

Complement (C) activation can underlie the infusion reactions to liposomes and other nanoparticle-based medicines, a hypersensitivity syndrome that can be partially reproduced in animal models. However, the sensitivities and manifestations substantially differ in different species, and C activation may not be the only cause of pathophysiological changes. In order to map the species variation of C-dependent and -independent pseudoallergy (CARPA/CIPA), here we used known C activators and C activator liposomes to compare their acute hemodynamic, hematological, and biochemical effects in rats. These C activators were cobra venom factor (CVF), zymosan, AmBisome (at 2 doses), its amphotericin B-free vehicle (AmBisombo), and a PEGylated cholesterol-containing liposome (PEG-2000-chol), all having different powers to activate C in rat blood. The pathophysiological endpoints measured were blood pressure, leukocyte and platelet counts, and plasma thromboxane B2, while C activation was assessed by C3 consumption using the Pan-Specific C3 assay. The results showed strong linear correlation between C activation and systemic hypotension, pointing to a causal role of C activation in the hemodynamic changes. The observed thrombocytopenia and leukopenia followed by leukocytosis also correlated with C3 conversion in case of C activators, but not necessarily with C activation by liposomes. These findings are consistent with the double hit hypothesis of hypersensitivity reactions (HSRs), inasmuch as strong C activation can fully account for all symptoms of HSRs, but in case of no-, or weak C activators, the pathophysiological response, if any, is likely to involve other activation pathways.
Optimization of Production Parameters for Probiotic Lactobacillus Strains as Feed Additive
Authors: Ren Hao , Zentek Jürgen , Vahjen Wilfried
Published: September 2019

In animal nutrition, probiotics are considered as desirable alternatives to antibiotic growth promoters. The beneficial effects of probiotics primarily depend on their viability in feed, which demands technical optimization of biomass production, since processing and storage capacities are often strain-specific. In this study, we optimized the production parameters for two broiler-derived probiotic lactobacilli (L. salivarius and L. agilis). Carbohydrate utilization of both strains was determined and preferred substrates that boosted biomass production in lab-scale fermentations were selected. The strains showed good aerobic tolerance, which resulted in easier scale-up production. For the freeze-drying process, the response surface methodology was applied to optimize the composition of cryoprotective media. A quadratic polynomial model was built to study three protective factors (skim milk, sucrose, and trehalose) and to predict the optimal working conditions for maximum viability. The optimal combination of protectants was 0.14g/mL skim milk/ 0.08 g/mL sucrose/ 0.09 g/mL trehalose (L. salivarius) and 0.15g/mL skim milk/ 0.08 g/mL sucrose/ 0.07 g/mL (L. agilis), respectively. Furthermore, the in-feed stabilities of the probiotic strains were evaluated under different conditions. Our results indicate that the chosen protectants exerted an extensive protection on strains during the storage. Although only storage of the strains at 4 °C retained the maximum stability of both Lactobacillus strains, the employed protectant matrix showed promising results at room temperature.
Mapping and Predicting Patterns of Chinese Adolescents’ Food Preferences
Authors: Sun Shaojing , He Jinbo , Fan Xitao
Published: September 2019

This study aimed to examine the patterns of, as well as the predictors for, Chinese adolescents’ food preferences. Using the national data of the China Health and Nutrition Survey (CHNS), we analyzed the data of 697 adolescents in the age range of 12 to 17 years. Latent class analysis revealed four types of food preferences: varied diet (37.09%, n = 254), avoiding vegetables (19.69%, n = 131), low appetite (7.56%, n = 50), and healthy diet (35.66%, n = 222). Major predictors for food preferences included demographic variables (e.g., gender, urban versus rural residence), nutrition knowledge, preference for activities, and social attitudes. Results did not show any significant differences in BMI z-scores among the four latent classes. However, there were significant differences in the number of sleeping hours among the classes.
Functional Relationship between Leptin and Nitric Oxide in Metabolism
Authors: Becerril Sara , Rodríguez Amaia , Catalán Victoria , Ramírez Beatriz , Unamuno Xabier , Portincasa Piero , Gómez-Ambrosi Javier , Frühbeck Gema
Published: September 2019

Leptin, the product of the ob gene, was originally described as a satiety factor, playing a crucial role in the control of body weight. Nevertheless, the wide distribution of leptin receptors in peripheral tissues supports that leptin exerts pleiotropic biological effects, consisting of the modulation of numerous processes including thermogenesis, reproduction, angiogenesis, hematopoiesis, osteogenesis, neuroendocrine, and immune functions as well as arterial pressure control. Nitric oxide (NO) is a free radical synthesized from L-arginine by the action of the NO synthase (NOS) enzyme. Three NOS isoforms have been identified: the neuronal NOS (nNOS) and endothelial NOS (eNOS) constitutive isoforms, and the inducible NOS (iNOS). NO mediates multiple biological effects in a variety of physiological systems such as energy balance, blood pressure, reproduction, immune response, or reproduction. Leptin and NO on their own participate in multiple common physiological processes, with a functional relationship between both factors having been identified. The present review describes the functional relationship between leptin and NO in different physiological processes.
High-Molecular-Weight Polyphenol-Rich Fraction of Black Tea Does Not Prevent Atrophy by Unloading, But Promotes Soleus Muscle Mass Recovery from Atrophy in Mice
Authors: Aoki Yuki , Ozawa Tetsuo , Numata Osamu , Takemasa Tohru
Published: September 2019

Previously, we reported that polyphenol-rich fraction (named E80) promotes skeletal muscle hypertrophy induced by functional overload in mice. This study indicates that E80 has potential for affecting skeletal muscle mass. Then, we evaluate the effect of E80 on atrophic and recovery conditions of skeletal muscle in mice. Hindlimb suspension (unloading) and relanding (reloading) are used extensively to observe disuse muscle atrophy and subsequent muscle mass recovery from atrophy. Eight-week old C57BL/6 mice were fed either a normal diet or a diet containing 0.5% E80 for two weeks under conditions of hindlimb suspension and a subsequent 5 or 10 days of reloading. We found that E80 administration did not prevent atrophy during hindlimb suspension, but promoted recovery of slow-twitch (soleus) muscle mass from atrophy induced by hindlimb suspension. After five days of reloading, we discovered that phosphorylation of the Akt/mammalian target of rapamycin (mTOR) pathway proteins, such as Akt and P70 ribosomal protein S6 kinase (S6K), was activated in the muscle. Therefore, E80 administration accelerated mTOR signal and increased protein synthesis in the reloaded soleus muscle.
Nutritional Status of Children with Cerebral Palsy—Findings from Prospective Hospital-Based Surveillance in Vietnam Indicate a Need for Action
Authors: Karim Tasneem , Jahan Israt , Dossetor Rachael , Giang Nguyen Thi Huong , Van Anh Nguyen Thi , Dung Trinh Quang , Chau Cao Minh , Van Bang Nguyen , Badawi Nadia , Khandaker Gulam , Elliott Elizabeth
Published: September 2019

Background: Lack of evidence on the burden and risk factors for malnutrition among children with cerebral palsy (CP) in Vietnam limits evidence-based interventions. We aimed to define the nutritional status of children with CP in Vietnam. Materials and Methods: The study utilized data from active prospective hospital-based surveillance modelled on the Pediatric Active Enhanced Disease Surveillance system. Children (0–18 years) with CP attending the National Children’s Hospital Hanoi, Vietnam between June–November 2017 were included. Data on demographic, clinical and rehabilitation status were collected following detailed neurodevelopmental assessment. Anthropometric measurements were taken. Nutritional status was determined using the World Health Organization guideline. Results: Of 765 children (the mean (SD) age was 2.6 (2.5) years; 35.8% were female), 28.9% (n = 213) were underweight and 29.0% (n = 214) stunted. The odds of underweight were significantly higher among children aged >5 years and/or having a monthly family income of <50 USD. Underweight and/or stunting was high among children with quadriplegia (81%, n = 60 and 84.5%, n = 87) and/or Gross Motor Functional Classification System (GMFCS) level IV–V (62.5%, n = 45 and 67.0%, n = 67). Nearly one-third of intellectually impaired and more than half of hearing-impaired children were underweight and/or stunted. Conclusions: Poor economic status and increased motor severity increased vulnerability to malnutrition. Our findings will inform nutritional rehabilitation programs among these vulnerable children.