bluuebunny/crossref_metadata_2025 huggingface dataset:
Radiation Dose in Coronary Angiogram: A Comparison of Radial and Femoral Approach
Authors: Sirikhamkorn Chulaporn , Soontornmanokati Natcha , Meemook Krissada
Published: September 2023

Background: Many clinical trials have demonstrated the benefit of trans-radial access (TRA) over trans-femoral access (TFA) in reducing mortality and bleeding events, but there is some concern about radiation exposure with radial access.
Objective: To assess the difference in radiation exposure between radial and femoral approach as measured by dose-area product (DAP) and fluoroscopy time.
Methods: This cross-sectional study was performed in patients aged 15 years and above who underwent invasive percutaneous coronary angiography at Ramathibodi Hospital between December 2019 and December 2020. Exclusion criteria included previous coronary artery bypass graft, unstable hemodynamic status during procedure, and changing the access site or using access sites other than femoral or radial arteries. Demographic data of patients was collected through medical records. DAP and fluoroscopy time of patients with radial and femoral approach were evaluated.
Results: Of 427 patients (49.4% male wtih mean age of 65.7 years), 245 (57.4%) patients underwent TFA. There was no significant difference in the median DAP between the radial and femoral approach (radial 1838 mGy.cm2 vs femoral 1690.7 mGy.cm2, P = .31). However, the fluoroscopy time was significantly higher in radial approach group (radial 4.6 min vs femoral 3.3 min, P < .001).
Conclusions: The radiation exposure was not significantly different between trans-radial and trans-femoral coronary angiography.
Kültürel Irkçılık Bağlamında İslamofobi ve Antisemitizm İlişkisi
Authors: AR Merve
Published: September 2023

Irkçılık, nefret, soykırım, yabancı düşmanlığı gibi tüm ayrımcılık biçimlerini içine alan tarihsel aşamalar “ötekileştirme” ile başlar. Tarih boyunca “öteki” üzerinden yükselme ve kimlik inşası oluşturma çabasında olan Batı medeniyeti Avrupa’nın özünü oluşturan değerler üzerinden, kendinden olmayan kültürlere “öteki” muamelesi yapmaktadır. Bu açıdan Batı medeniyetinin çok kültürlülüğü ve kendinden olmayanları ötekileştiren monolitik yapısı genelde yabancı düşmanlığına, özelde ise İslamofobiye sebep olmaktadır. İslamofobi kavramı tarihî, teolojik ve siyasi temelleri asla göz ardı edilmemekle birlikte özellikle ırkçılık ve kültürel ırkçılık bağlamında da yeniden değerlendirilmesi gereken bir kavram olarak karşımızda durmaktadır.
Bu çalışmamızda Müslümanların hem ötekileştirilmesi hem de şiddet ve terörle bağdaştırılmasının gerekçelerinden biri olarak gördüğümüz ırk ve ırkçılık anlayışı ve bu anlayışın en somut ürünü olan zenofobik tutum ve İslamofobinin ırkçılığın yeni bir kolu olan kültürel ırkçılık bağlamında değerlendirilip değerlendirilmeyeceği meselesi tartışılmıştır. Bununla birlikte bu öteki anlayışında zaman zaman Müslümanlar ile Yahudiler arasında kurulmaya çalışan bir ilişki söz konusu olduğu için son zamanlarda “İslamofobi yeni antisemitizm” midir sorusu gündeme getirilmektedir. Biz de çalışmamızın ilerleyen kısımlarında İslamofobi ve antisemitizm kavramlarının aynı düzlemde değerlendirilip değerlendirilemeyeceği meselesini müzakere ettik ve bu benzerliğin pratikte stratejik bir karşılığı olabilir mi sorusuna cevap aradık.
Reabilitação oral utilizando prótese obturadora palatina em paciente acometido por carcinoma de células escamosas: relato de caso
Authors: Vasconcelos Ricardo Anderson de Oliveira , Jardim Juscelino de Freitas , Lima Ezequiel Sales de , Klen Graziele de Lima , Sousa Thiago Fonteles de , Sá Cícero Alexandre Oliveira , Wenzel Giovanna Déa Mitre , Faustino André Fortuna de Albuquerque Câmara , Wenzel Netto Arlindo
Published: September 2023

Introdução: O termo "câncer de boca" engloba uma série de neoplasias que afetam a cavidade oral. O carcinoma de células escamosas (CEC) é o tipo mais comum de câncer maligno oral, responsável por mais de 90% de todos os tumores malignos nessa região. Os tumores localizados no palato mole e na orofaringe geralmente são maiores em tamanho em comparação com os carcinomas que afetam os lábios e a língua. O tratamento cirúrgico é a opção mais indicada para essa patologia, embora possa ser agressivo, afetando as estruturas bucais adjacentes, principalmente quando envolve as áreas mais posteriores. Essa abordagem cirúrgica pode resultar em distúrbios funcionais, como dificuldades na fala, mastigação e deglutição. Objetivo: Este trabalho tem como objetivo relatar um caso de reabilitação oral de um paciente submetido a tratamento oncológico, após ser diagnosticado com carcinoma de células escamosas. O paciente foi submetido a procedimentos cirúrgicos, incluindo esvaziamento cervical radical à esquerda, ressecção de tumor no palato, pelveglossomandibulectomia, ressecção de faringe e enxerto de pele braquial. Considerações finais: A abordagem multidisciplinar é essencial para restabelecer a qualidade de vida do paciente após o tratamento oncológico. O cirurgião-dentista desempenha um papel fundamental na minimização das sequelas causadas pelo tratamento. A confecção de uma prótese obturadora geralmente permite a reabilitação funcional e estética satisfatória, sem a necessidade de procedimentos invasivos adicionais.
Morbimortalidade hospitalar da sífilis no Brasil de 2012 a 2021
Authors: Borges Alice Lima , Gonçalves João Lucas Gigante , Kós Bianca Marçal , Ferreira Larissa Pereira , Pinheiro André Vitor Ribeiro , Ribeiro Raul Felipe Santos , Soares Beatriz Lima , Aragão Gracielly Maria Marinho
Published: September 2023

A sífilis é uma Infecção Sexualmente Transmissível (IST) que representa um importante problema de saúde pública. Tal patologia pode acometer todas as faixas etárias e está presente em todos os países, independentemente de seu desenvolvimento econômico e social. Apesar disso, a maioria dos casos relacionados à sífilis ocorre em países de média e baixa renda, havendo variações de prevalência e incidência dentro de cada país. No Brasil, estudos sobre a morbimortalidade hospitalar por sífilis são escassos, de amplitude local ou restritos a curto período de análise. Este estudo se propôs a traçar o perfil de morbimortalidade hospitalar da sífilis no país. Trata-se de estudo ecológico com dados da sífilis entre os anos de 2012 e 2021. Os dados foram coletados no sistema de informação do SUS - TABNET DATASUS, segundo variáveis sociodemográficas. Além disso, o presente trabalho visa analisar as desigualdades socioeconômicas e de acesso e uso de serviços de saúde e sua relação com as desigualdades nos riscos de morte entre as regiões, UF e grupos de sexo e idade do país, visto que conhecer tal perfil, permite um melhor manejo da doença, através do embasamento epidemiológico, o qual viabiliza melhorias na implantação e desenvolvimento de políticas públicas de saúde voltadas para a prevenção, diagnóstico e tratamento da sífilis.
Uma aplicação do SPI (Standardized Precipitation Index) para dados de chuvas mensais em Pernambuco entre 1991-2019
Authors: Felipe Viviane Farias , Santos Jefferson Vieira dos , Barbosa Nyedja Fialho Morais , Xavier Erika Fialho Morais , Xavier Júnior Sílvio Fernando Alves , Jale Jader da Silva
Published: September 2023

A determinação e classificação de regiões propensas a eventos climáticos críticos, tanto de chuvas intensas quanto de períodos de seca é de suma importância no âmbito da variabilidade climática. No Nordeste brasileiro, uma região semiárida, a seca é um problema recorrente, enquanto eventos climáticos intensos, como chuvas fortes e deslizamentos de terra, afetam regiões metropolitanas e causam desastres. O estado de Pernambuco apresenta tendência de eventos climáticos extremos, com longos períodos de seca e chuvas intensas, que é responsável por numerosos desastres naturais no estado. O objetivo do trabalho foi analisar e classificar, de forma objetiva, a variabilidade climática entre 1991 e 2019 em cinco estações de Pernambuco localizadas nas diferentes mesorregiões do Estado representadas por: Recife, Palmares, Itaíba, Salgueiro e Petrolina, com base em diferentes escalas temporais (1, 3, 6, 9, 12, 24 e 36 meses). Para isso, utilizou-se o Índice de Precipitação Padronizado (SPI) desenvolvido para classificar condições secas e úmidas de acordo com severidade. Através da análise observou-se que as escalas menores SPI-1 e SPI-3 revelaram o início e a trajetória de cada evento, enquanto as demais escalas identificaram os eventos mais intensos e prolongados. Os resultados obtidos indicaram que os períodos de secas apresentaram maior duração e intensidade, sendo o mês mais seco em Itaíba -4.416 (SPI-3) em agosto de 2018, porém os períodos de chuvas tiveram maior ocorrência nas estações, o mês mais úmido ocorreu em Palmares 2.928 (SPI-3) em setembro de 2000.
Composição nutricional e padrão microbiológico de pães franceses enriquecidos com extrato da polpa do fruto de buriti (Mauritia flexuosa M.)
Authors: Barbosa Antonio Marcel da Rocha , Silva Reginaldo Ferreira da , Imada Katiuscia Shirota , Pinto Wagner de Jesus , Nunes Graciele Lorenzoni
Published: September 2023

O buriti é utilizado como alimento em várias receitas culinárias pela população Amazônica. A casca e a polpa do fruto contêm minerais, fibras dietéticas, óleos essências e carotenóides totais. Sendo assim, este trabalho objetivou enriquecer a composição nutricional do pão francês que tem como nutriente predominante carboidratos através da adição do extrato aquoso da polpa do fruto. Após pré-testes, determinou-se as concentrações de 50, 100 e 150 mL de extrato a ser adicionado na mistura com a farinha de trigo para o preparo da massa. No extrato da polpa de buriti, bem como, nos pães fórmulados com as diferentes concentrações de extrato, realizaram-se análises de composição centesimal, compostos funcionais e padrão microbiológicos. Os pães padrão e experimentais, apresentaram composição centesimal semelhante para os teores de cinzas, proteínas e fibra alimentar. Porém, os pães contendo extrato de buriti apresentaram menores teores de lipídeos, carboidratos e valor calórico, em relação ao pão francês padrão. Análises microbiológicas mostraram segurança alimentar adequada, tendo em vista a ausência dos microoganismos analisados, credenciando os mesmos a serem utilizados como alimento nutricional e saudável e assim, propiciar benefícios a saúde do consumidor.
Métodos inovadores para o tratamento do pé diabético: Uma revisão de literatura
Authors: Lima Lívia Cardoso , Bitencourt  Joana Alves , Radmann Guilherme Corrêa , Farias Any Eduarda Nanes de Oliveira , Carvalho Felipe Mendes de Andrade de
Published: September 2023

Objetivo: Investigar os tratamentos inovadores e promissores para o tratamento do pé diabético. Metodologia: Para responder a questão norteadora “Quais são os métodos mais modernos e disponíveis para o tratamento do pé diabético?”, foi realizado uma revisão integrativa, a partir do levantamento de artigos nas bases de dados SciELO, PubMed e Google Scholar, utilizando como descritores “Diabetes Mellitus”, “Pé diabético”, Cicatrização de feridas”, "Terapêutica", "Diabetic foot”, “Wound healing” e “Therapy” Os critérios de inclusão foram: artigos originais, publicados no período de 2017 a 2023, nos idiomas português e inglês. Os critérios de exclusão englobam os artigos que não tinham relação com o objeto de estudo, resumos simples, capítulos de livros, fichas técnicas ou relatos de experiência. Resultados: Foram incluídos 34 artigos. Dentre eles foram selecionados aqueles que abordavam os tratamentos alternativos a partir de substratos naturais como, mel de grau médico, biomembranas de látex, biomembrana com extrato de Actinidia deliciosa (kiwi), placenta humana e seus derivados. Além de outros cuidados para a cicatrização de feridas do pé diabético, como a ozonioterapia, oxigenoterapia hiperbárica, irradiação ultrassônica, espuma de feltro, pressão negativa à vácuo, oxigênio tópico e a fototerapia. Conclusão: Por meio dessa revisão integrativa, destaca-se a importância de compreender os diferentes tipos de tratamentos utilizados no pé diabético, de forma a escolher o mais adequado e eficiente para cada caso dessa complicação crônica.
TARI TOPENG PAMINDU NINI ASTALIAH DI DESA BARIKIN
Authors: Silvina Della
Published: September 2023

Penelitian ini berkaitan dengan Tari Topeng Pamindu Nini Astaliah Di Desa Barikin. Tari topeng berkembang di barikin sekitar tahun 1380 pada saat Kerajaan Dipa topeng memiliki 7 jenis adapun topeng yang paling sering nini astaliah tarikan adalah Topeng Pamindu. Tujuan Penelitian ini : (1) Mendeskripsikan bentuk penyajian tari topeng pamindu nini astaliah, (2) Mendeskripsikan fungsi tari topeng pamindu nini astaliah. Teknik pengumpulan data menggunakan teknik observasi, wawancara dan dokumentasi. Hasil Penelitian yang diperoleh adalah, bentuk penyajian Tari Topeng Pamindu Gerak , Iringan/Musik , Tata Rias,Tata Busana, Pola Lantai, Tempat Pertunjukan, Properti, Tema, Tata Lampu tema tari topeng pamindu adalah upacara ritual gerak topeng pamindu terdapat 16 ragam gerak iringan musik menggunakan seperangkat alat musik gamelan banjar ada 8 alat musik yang dimainkan tata rias menggunakan rias natural.baju poko berwarna kuning, ,tapih sasirangan, kida- kida,selendang, ikat pinggang, kalung, anting, gelanggelang kaki giring-girimg dan subrah. Pola lantai yang digunakan adalah maju mundur ke samping kiri ke samping kanan lurus, tempat pertunjukan yang digunakan adalah panggung terbuka dan panggung proscenium. Properti topeng pamindu dan kain penutup topeng berwarna kuning tata lampu yang digunakan dalam tari topeng pamindu adalah cahaya matahari.
Role and Competencies Development of Librarians in Digital Era
Authors: Mustar Maniso , Rahmadanita Annisa
Published: September 2023

Background: Technology advancement has experienced a rapid increase in digital era, but not all librarians possess the qualified competencies to address the associated challenges.

Objective: This study aims to analyze role and competency development of contemporary librarians in the digital era.

Method: The methodology used includes bibliographic content analysis and descriptive study.

Results: The result showed that contemporary librarians should take the initiative to meet information requirements of users. Librarians need to stay abreast of information technology advancement, keep pace with development in digital libraries, act as problem solvers, and enhance their skills. The discussion concerning role and competencies of contemporary librarians showed that they should serve as trailblazers in the field of information and technology.

Conclusion: In conclusion, competencies required for contemporary librarians in digital era include the ability to analyze and use information, manage time, and use information technology. Contemporary librarians must be capable of collaborating with other staff to achieve organizational goals

Keywords: contemporary librarians; information; competencies; information technology.

 

 

Abstrak

 Latar Belakang: Kemajuan teknologi mengalami peningkatan pesat di era digital, namun tidak semua pustakawan memiliki kompetensi yang mumpuni untuk menjawab tantangan terkait.

Tujuan: Penelitian ini bertujuan untuk menganalisis peran dan pengembangan kompetensi pustakawan masa kini di era digital.

Metode: Metodologi yang digunakan meliputi analisis isi bibliografi dan studi deskriptif.

Hasil: Hasil penelitian menunjukkan bahwa pustakawan masa kini harus mengambil inisiatif untuk memenuhi kebutuhan informasi pengguna. Pustakawan perlu mengikuti kemajuan teknologi informasi, mengikuti perkembangan perpustakaan digital, bertindak sebagai pemecah masalah, dan meningkatkan keterampilan mereka. Pembahasan mengenai peran dan kompetensi pustakawan masa kini menunjukkan bahwa mereka harus menjadi pelopor di bidang informasi dan teknologi.

Kesimpulan: Kompetensi yang diperlukan bagi pustakawan masa kini di era digital antara lain kemampuan menganalisis dan menggunakan informasi, mengatur waktu, dan memanfaatkan teknologi informasi. Pustakawan masa kini harus mampu berkolaborasi dengan staf lain untuk mencapai tujuan organisasi

 Kata Kunci: pustakawan kontemporer; informasi; kompetensi; teknologi Informasi.
The effects of DC offset in direct-conversion receivers for WLAN systems
Authors: ŞEN Yunus Emre , TAŞCIOĞLU Selçuk
Published: September 2023

In this paper, the effects DC offset in direct-conversion receivers for WLAN systems are analyzed using both experimental and simulation data. DC offset estimation is performed by using data-aided methods which are based on the short training sequence of WLAN preamble. In the simulations, DC offset and frequency offset estimations are carried out on the signals affected by frequency selective Rayleigh fading channel and additive white Gaussian noise. Estimation performance of the methods is compared for different SNR levels and frequency offset values in terms of mean square error. Experimental data which is in the form of WLAN packets and transmitted through a wireless channel is captured by using a software defined radio. The experimental performance of the DC offset compensation methods is evaluated in terms of transmission success ratio.
Airborne imagery and lidar based 3D reconstruction using commercial drones
Authors: AÇICI Koray , ERDAL Ömer Mert , YILMAZ Alperen , UNAL Metehan , BOSTANCI Gazi Erkan , GÜZEL Mehmet Serdar
Published: September 2023

In the study, the implementation of 3D reconstruction of buildings using drones is explained. In this project, Airsim was used as the simulation environment and images were obtained from the simulation environment using OpenCV and the Meshroom software was run on these images and modeling was done in the computer environment. For real-world studies, the engineering faculty in Ankara University 50. Yıl Campus was modeled using photogrammetry technique. In the last part, the results of different modelling algorithms were compared.
Estimating the frequency and bandwidth of square-split ring resonator (S-SRR) designs via support vector machines (SVM)
Authors: CAN Sultan , SOYSAL Gökhan
Published: September 2023

In this study Support Vector Machine (SVM) based estimation technique is proposed for estimating the bandstop frequency and bandwidth of square-split ring resonators. Artificially engineered surfaces especially the planar frequency selective surfaces like the SRRs have narrowband properties so that estimating the filtering frequencies and the bandwidth is essential in a cost and design-effective way. The proposed method, which is superior to optimization methods and 3D electromagnetic solvers in terms of cost and computational burden, achieved accurate results via SVMs generalization capability. This study represents two SVM regression models one for predicting frequency and the other for predicting bandwidth having fast response and accuracy. Results of the proposed model reveal that resonance frequency estimation error, in terms of percentage, is bounded in the interval [0.0542, 3.5938], with an overall error of 0.89 % for the test data. The mean and standard deviation of the percentage error is obtained as 0.9861 and 0.9376, respectively. In addition to that -10dB bandwidth is estimated with the bounded error where estimation error in terms of percentage would be lie in the interval [0.068925, 6.876800] with an overall error of 3.68% for the test data.
Geothermal Power Disputes: Quantifying Production Loss
Authors: Bernardo Darren
Published: September 2023

Commonly, the projected power output and subsequent profitability of a geothermal power plant is based on data taken from the feasibility study provided to the project’s lenders during the exploratory stage of the project. Due to the upfront investment risks in geothermal power plants, it is largely accepted that the main purpose of a feasibility study is to build a business case for funding support from private, public, or institutional bodies. In this paper I highlight the pitfalls of relying on traditional feasibility study data for geothermal power plants and give examples of various post-feasibility study factors that directly affect profitability. This includes, but is not limited to; projected well production, ambient temperature, internal consumption, plant availability and government pricing legislation.
Evaluation of a New Mordant Based Haematoxylin Dye (Haematoxylin X) for Use in Clinical Pathology
Authors: Gabriel J. A. , D’Amico C. , Kosgodage U. , Satoc J. , Haine N. , Willis S. , Orchard G. E.
Published: September 2023

Recently, St John’s Dermatopathology Laboratory and CellPath Ltd have developed a new patented haematoxylin dye (Haematoxylin X) that utilises a chromium-based mordant (Chromium Sulphate). In this study, the performance of this new haematoxylin (Haematoxylin X) was compared against some commonly utilised alum-based haematoxylins (Carazzi’s, Harris’ and Mayer’s) when used as a part of formalin-fixed paraffin embedded (FFPE) tissue, special stains, immunohistochemical counterstaining and frozen section (Mohs procedure) staining procedures. FFPE sections of different tissue types and frozen skin tissues were sectioned and stained with each haematoxylin subtype to allow for a direct comparison of staining quality. The slides were independently evaluated microscopically by two assessors. A combined score was generated to determine the sensitivity (defined as the intensity of haematoxylin staining being too weak or too strong and the colour of the haematoxylin staining not being blue/black) and specificity (defined as the presence of haematoxylin background staining, uneven staining, and staining deposits) for each of the four haematoxylin subtypes. The scoring criteria were based on the UKNEQAS Cellular pathology techniques assessment criteria. In FFPE tissue, the results for specificity identified Harris haematoxylin scoring the highest (91.2%) followed by Haematoxylin X (88.0%) and Mayer’s (87.0%). The sensitivity scores again identified Harris haematoxylin as scoring the highest (95.1%) followed by Haematoxylin X (90.0%) and Mayer’s (88.0%). In frozen tissue, the results for specificity identified Haematoxylin X as scoring the highest (85.5%) followed by Carazzi’s (80.7%) and Harris’ (77.4%). The sensitivity scores again identified Haematoxylin X as scoring the highest (86.8%) followed by Carazzi’s (82.0%) and Harris’ (81.0%). The results achieved with all four haematoxylins showed a high degree of comparability, with Harris’ haematoxylin scoring high scores overall compared to the other four when assessing FFPE sections. This may have been due to familiarity with the use of Harris’ haematoxylin in-house. There was also evidence of more pronounced staining of extracellular mucin proteins with Haematoxylin X compared to the other alum haematoxylins that were assessed. Haematoxylin X scored highest when used in frozen section staining. In addition, Haematoxylin X has a potential applications for use in IHC and special stains procedures as a counterstain.
Potential involvement of the bone marrow in experimental Graves’ disease and thyroid eye disease
Authors: Gulbins Anne , Horstmann Mareike , Keitsch Simone , Soddemann Matthias , Wilker Barbara , Wilson Gregory C. , Zeidan Ryan , Hammer Gary D. , Daser Anke , Bechrakis Nikolaos E. , Görtz Gina-Eva , Eckstein Anja
Published: September 2023

Introduction

Graves’ disease is an autoimmune disorder caused by auto-antibodies against the thyroid stimulating hormone receptor (TSHR). Overstimulation of the TSHR induces hyperthyroidism and thyroid eye disease (TED) as the most common extra thyroidal manifestation of Graves’ disease. In TED, the TSHR cross talks with the insulin-like growth factor 1 receptor (IGF-1R) in orbital fibroblasts leading to inflammation, deposition of hyaluronan and adipogenesis. The bone marrow may play an important role in autoimmune diseases, but its role in Graves’ disease and TED is unknown. Here, we investigated whether induction of experimental Graves’ disease and accompanying TED involves bone marrow activation and whether interference with IGF-1R signaling prevents this activation.

Results

Immunization of mice with TSHR resulted in an increase the numbers of CD4-positive T-lymphocytes (p ≤0.0001), which was normalized by linsitinib (p = 0.0029), an increase of CD19-positive B-lymphocytes (p= 0.0018), which was unaffected by linsitinib and a decrease of GR1-positive cells (p= 0.0038), which was prevented by linsitinib (p= 0.0027). In addition, we observed an increase of Sca-1 positive hematopietic stem cells (p= 0.0007) and of stromal cell-derived factor 1 (SDF-1) (p ≤0.0001) after immunization with TSHR which was prevented by linsitinib (Sca-1: p= 0.0008, SDF-1: p ≤0.0001). TSHR-immunization also resulted in upregulation of CCL-5, IL-6 and osteopontin (all p ≤0.0001) and a concomitant decrease of the immune-inhibitory cytokines IL-10 (p= 0.0064) and PGE2 (p ≤0.0001) in the bone marrow (all p≤ 0.0001). Treatment with the IGF-1R antagonist linsitinib blocked these events (all p ≤0.0001). We further demonstrate a down-regulation of arginase-1 expression (p= 0.0005) in the bone marrow in TSHR immunized mice, with a concomitant increase of local arginine (p ≤0.0001). Linsitinib induces an upregulation of arginase-1 resulting in low arginase levels in the bone marrow. Reconstitution of arginine in bone marrow cells in vitro prevented immune-inhibition by linsitinib.

Conclusion

Collectively, these data indicate that the bone marrow is activated in experimental Graves’ disease and TED, which is prevented by linsitinib. Linsitinib-mediated immune-inhibition is mediated, at least in part, by arginase-1 up-regulation, consumption of arginine and thereby immune inhibition.
Carbon and hydrogen stable isotope fractionation due to monooxygenation of short-chain alkanes by butane monooxygenase of Thauera butanivorans Bu-B1211
Authors: Vogt Carsten , Song Zhiyong , Richnow Hans-Hermann , Musat Florin
Published: September 2023

Multi element compound-specific stable isotope analysis (ME-CSIA) is a tool to assess (bio)chemical reactions of molecules in the environment based on their isotopic fingerprints. To that effect, ME-CSIA concepts are initially developed with laboratory model experiments to determine the isotope fractionation factors specific for distinct (bio)chemical reactions. Here, we determined for the first time the carbon and hydrogen isotope fractionation factors for the monooxygenation of the short-chain alkanes ethane, propane, and butane. As model organism we used Thauera butanivorans strain Bu-B1211 which employs a non-haem iron monooxygenase (butane monooxygenase) to activate alkanes. Monooxygenation of alkanes was associated with strong carbon and hydrogen isotope effects: εbulkC = −2.95 ± 0.5 ‰ for ethane, −2.68 ± 0.1 ‰ for propane, −1.19 ± 0.18 ‰ for butane; εbulkH = −56.3 ± 15 ‰ for ethane, −40.5 ± 2.3 ‰ for propane, −14.6 ± 3.6 ‰ for butane. This resulted in lambda (Λ ≈ εHbulk/εCbulk) values of 16.2 ± 3.7 for ethane, 13.2 ± 0.7 for propane, and 11.4 ± 2.8 for butane. The results show that ME-CSIA can be used to track the occurrence and impact of monooxygenase-dependent aerobic processes converting short-chain alkanes in natural settings like marine and terrestrial seeps, gas reservoirs, and other geological formations impacted by natural gas.
Neural sensory stimulation does not interfere with the H-reflex in individuals with lower limb amputation
Authors: Li Suzhou , Triolo Ronald J. , Charkhkar Hamid
Published: September 2023

Introduction

Individuals with lower limb loss experience an increased risk of falls partly due to the lack of sensory feedback from their missing foot. It is possible to restore plantar sensation perceived as originating from the missing foot by directly interfacing with the peripheral nerves remaining in the residual limb, which in turn has shown promise in improving gait and balance. However, it is yet unclear how these electrically elicited plantar sensation are integrated into the body’s natural sensorimotor control reflexes. Historically, the H-reflex has been used as a model for investigating sensorimotor control. Within the spinal cord, an array of inputs, including plantar cutaneous sensation, are integrated to produce inhibitory and excitatory effects on the H-reflex.

Methods

In this study, we characterized the interplay between electrically elicited plantar sensations and this intrinsic reflex mechanism. Participants adopted postures mimicking specific phases of the gait cycle. During each posture, we electrically elicited plantar sensation, and subsequently the H-reflex was evoked both in the presence and absence of these sensations.

Results

Our findings indicated that electrically elicited plantar sensations did not significantly alter the H-reflex excitability across any of the adopted postures.

Conclusion

This suggests that individuals with lower limb loss can directly benefit from electrically elicited plantar sensation during walking without disrupting the existing sensory signaling pathways that modulate reflex responses.
Protective effect of heat-processed Gynostemma pentaphyllum on high fat diet-induced glucose metabolic disorders mice
Authors: Xie Jin-Bo , Xie Peng , Guo Mei , Li Fang-Fang , Xiao Man-Yu , Qi Yan-Shuang , Pei Wen-Jing , Luo Hao-Tian , Gu Yu-Long , Piao Xiang-Lan
Published: September 2023

Glucose metabolic disorders (GMD) can promote insulin resistance (IR) and diabetes, and damage liver and kidney. Gynostemma pentaphyllum is commonly used in the clinical treatment of diabetes, but the research on its main active constituents and GMD has not been reported yet. This study explores the therapeutic potential of gypenosides of heat-processed Gynostemma pentaphyllum (HGyp) on high-fat diet-induced GMD in mice. HGyp was administered at different doses for 12 weeks. The investigation encompassed an array of parameters, including body weight, blood lipids, blood glucose, and liver tissue components. Metabolomic and network analyses were conducted to uncover potential targets and pathways associated with HGyp treatment. The results revealed that HGyp alleviated GMD by reducing body weight, blood glucose, and improving blood lipids levels, while increasing liver glycogen and antioxidant enzyme levels. Additionally, HGyp exhibited protective effects on liver and kidney health by reducing tissue damage. Fourteen blood components were detected by LC-MS. Metabolomic and network analyses indicated the potential engagement of the AGE-RAGE signaling pathway in the therapeutic effects of HGyp.Furthermore, Western blot and ELISA assays confirmed that HGyp upregulated GLO1 and GLUT4 while down-regulating AGEs and RAGE expression in liver tissue. In light of these findings, HGyp demonstrates promise as a potential therapeutic candidate for combating GMD, warranting further exploration in the development of therapeutic strategies or functional products.
A glycosylation risk score comprehensively assists the treatment of bladder neoplasm in the real-world cohort, including the tumor microenvironment, molecular and clinical prognosis
Authors: Liu Jinhui , He Yunbo , Zhou Weimin , Tang Zhuoming , Xiao Zicheng
Published: September 2023

Background: Bladder cancer is a common urological cancer associated high significant morbidity and mortality rates. Immunotherapy has emerged as a promising treatment option, although response rates vary among patients. Glycosylation has been implicated in tumorigenesis and immune regulation. However, our current comprehensive understanding of the role of glycosylation in bladder cancer and its clinical implications is limited.

Methods: We constructed a training cohort based on the downloaded TCGA-BLCA dataset, while additional datasets (Xiangya cohort, GSE32894, GSE48075, GSE31684, GSE69795 and E-MTAB-1803) from Xiangya hospital, GEO and ArrayExpress database were obtained and used as validation cohorts. To identify glycosylation-related genes associated with prognosis, univariate Cox regression and LASSO regression were performed. A Cox proportional hazards regression model was then constructed to develop a risk score model. The performance of the risk score was assessed in the training cohort using Kaplan-Meier survival curves and ROC curves, and further validated in multiple validation cohorts.

Results: We classified patients in the training cohort into two groups based on glycosylation-related gene expression patterns: Cluster 1 and Cluster 2. Prognostic analysis revealed that Cluster 2 had poorer survival outcomes. Cluster 2 also showed higher levels of immune cell presence in the tumor microenvironment and increased activation in key steps of the cancer immune response cycle. We developed an independent prognostic risk score (p < 0.001) and used it to construct an accurate prognostic prediction nomogram. The high glycosylation risk score group exhibited higher tumor immune cell infiltration, enrichment scores in immune therapy-related pathways, and a tendency towards a basal subtype. Conversely, the low-risk score group had minimal immune cell infiltration and tended to have a luminal subtype. These findings were consistent in our real-world Xiangya cohort.

Conclusion: This multi-omics glycosylation score based on these genes reliably confirmed the heterogeneity of bladder cancer tumors, predicted the efficacy of immunotherapy and molecular subtypes, optimizing individual treatment decisions.
Maximizing analytical precision: exploring the advantages of ratiometric strategy in fluorescence, Raman, electrochemical, and mass spectrometry detection
Authors: Madhu Manivannan , Santhoshkumar S. , Tseng Wei-Bin , Tseng Wei-Lung
Published: September 2023

Ratiometric strategy are an invaluable method that helps to detect and quantify analytes. This approach relies on measuring changes in the ratio of two or more signals to improve the accuracy and sensitivity of the results. Ratiometric strategies are widely used in a variety of fields including biomedical, environmental monitoring and food safety. It is particularly popular when traditional single-signal based detection methods are not feasible, especially when interfering substances severely affect the detection. In addition, ratiometric methods have the potential to improve the accuracy and reliability of analyte detection, leading to better results in a variety of complex environments. The article provides a comprehensive review of ratiometric strategy, focusing on ratiometric fluorescent nanoprobes for the visual detection of analytes. This paper also discusses the design of ratiometric two-photon fluorescent probes for biomedical imaging, the synthesis of ratiometric surface-enhanced Raman scattering nanoprobes for the imaging of intracellular analytes, the development of ratiometric molecularly imprinted electrochemical sensors for detection of electroactive species, and the use of isotopically-labeled internal standards in matrix-assisted laser desorption/ionization for ratiometric analysis. The article not only discusses each technique in detail, including its principles, advantages, potential applications, and limitations, but also highlights recent advances in each method and possible future directions.
Thoracolumbar Retrolaminar Block: Anatomical and Radiological Study of Injectate Pattern Distribution in Canine Cadavers
Authors: Pentsou Julia , Vagias Michail , Davies Thomas , Hoey Séamus , Huuskonen Vilhelmiina
Published: September 2023

The retrolaminar block is a regional anaesthetic technique, first developed in humans, in which the local anaesthetic is deposited directly onto the dorsal aspect of the thoracic or lumbar vertebral lamina. This study aims to evaluate the distribution of landmark-guided thoracolumbar retrolaminar injections in greyhound cadavers. Thirteen injections of contrast-dye solution were performed in eight cadavers at the level of the twelfth thoracic vertebra (T12), with either 20 mL (n = 8, high volume, HV) or 10 mL (n = 5, low volume, LV) per site. The spread of the injectate was evaluated through computed tomography and transverse anatomical dissection. The groups were compared using the Mann–Whitney U test. The median (range) of the extent of the spread was 4 (2–5) and 3 (2–4) intervertebral foramina in the LV and HV groups, respectively. The median (range) of the spread along the retrolaminar space was 3 (2–3) retrolaminar segments in the LV and 3 (2–4) in the HV group. Epidural and retroperitoneal spread was identified in seven cadavers. Following landmark-guided retrolaminar injections, the injectate spread both in the retrolaminar and paravertebral spaces, without any obvious association between the volume of injectate and the extent of the spread. Further studies are warranted to determine the clinical efficacy of the technique.
The Power Is in the Word—Do Laypeople Interpret Descriptors of Dog Emotional States Correctly?
Authors: Heritier Carmen , Riemer Stefanie , Gaschler Robert
Published: September 2023

A basic understanding of dog behaviour and emotion is relevant not only for professionals, such as veterinary personnel or dog trainers, but also for dog owners and for people with little contact with dogs. Information about dog behaviour and emotions is mostly conveyed verbally. This study explores whether definitions of dog behaviour and emotion are understood in such a way that they can be allocated to a descriptor (i.e., a label such as “fearful”), even by people with low background knowledge. If people can match descriptors to definitions, this suggests that the definitions are distinct enough and elicit mental representations of behaviour that can fit the label. Good agreement on the definitions is a prerequisite for the validity of the descriptors used; however, no study to date has tested this. A sample of 236 adults was asked to match descriptors of Qualitative Behaviour Assessment (QBA) for veterinary and shelter situations to their correct definitions, e.g., the descriptor “fearful” to its definition “dog may try to flee, hide or freeze; ears back”. Matching was substantially above chance; nonetheless, the mean proportion of correct responses was only 50% (SD ± 16.6%) for the veterinary QBA set and 33% (SD ± 14.3%) for the shelter QBA set. Performance in the matching task was positively correlated with measures of experience with dogs. Taken together, the results suggest that descriptor–definition pairs used to describe dog behaviour need to be clearly defined to avoid misinterpretations when teaching laypeople how to interpret canine behaviour.
Stability of Vitamin C Content in Plant and Vegetable Juices under Different Storing Conditions
Authors: Feszterová Melánia , Kowalska Małgorzata , Mišiaková Margaréta
Published: September 2023

The effect of environmental variables on vitamin C degradation in the analysed plant and vegetable juice samples was investigated. The study focused on samples from chives, dandelion, and nettle, as well as tomato, carrot, cucumber, red and white peppers, and cabbage. The aims of the study were to summarise the impact of storage time, temperature, and packaging materials on antioxidants (using vitamin C as an example) in processing plant and vegetable juices. The vitamin C concentration was tested iodometrically at 0 to 21 days intervals. Storage was carried out at different temperatures (4 °C, 23 °C, −18 °C) and in two selected packaging materials (glass and plastic). The analyses showed that low temperatures (t = 4 °C) and storage in glass containers are best for preserving vitamin C content. Storage time influenced the decrease in vitamin C values, with higher losses accompanying longer storage times. Plastic containers had a shorter shelf life for vitamin C compared to glass containers. In general, high temperatures during processing and storage have a negative effect on the preservation of vitamin C. Therefore, storing juices in the refrigerator and glass containers intended for food applications to minimise vitamin C degradation is important.
High-Performance Microcomputing Tomography of Chick Embryo in the Early Stages of Embryogenesis
Authors: Rzhepakovsky Igor , Piskov Sergei , Avanesyan Svetlana , Shakhbanov Magomed , Sizonenko Marina , Timchenko Lyudmila , Shariati Mohammad Ali , Rebezov Maksim , Nagdalian Andrey
Published: September 2023

X-ray contrast techniques were tested on the chick embryos in early periods of embryogenesis. For contrast stain, reagents with radiopacity in various concentrations were used: silver proteinate, eosin, Lugol’s solution (I2KI), phosphomolybdic acid and phosphotungstic acid under heating at 25 °C and 40 °C and exposure for 24 and 48 h. The use of silver proteinate, eosin and I2KI in various concentrations in the contrast of the chick embryo in the early period of embryogenesis did not make it possible to obtain microtomographic results that provide reliable microstructural analysis. The most optimal and effective method of embryo staining at the HH22–HH34 embryonic stages reliably determined the staining of 1% phosphotungstic acid at 40 °C heating and exposure for 24 h. Taking into account the size of the chick embryos and their structures at the HH22–HH34 embryonic stages, the features of the development, location of organs, and the minimum permissible parameters of microtomography for obtaining high-quality and reliable results were determined by the isometric spatial resolution of 8.87 μm, X-ray voltage 50 kV, X-ray current 500 μA, and the use of filters started from Al 0.5 mm. Microtomographic results were obtained, characterized by the appearance of the chick embryo at the HH22–HH34 embryonic stages, and they visualized the locations and structures of the chick embryo organs and provided calculation of their volume and X-ray density. The results of the work open up significant prospects for using the chick embryo at the early embryonic period of embryogenesis as an alternative model for screening teratogenicity.
The Effect of Rare-Earth Elements on the Morphological Aspect of Borate and Electrocatalytic Sensing of Biological Compounds
Authors: Morozov Roman , Stanković Dalibor , Avdin Viacheslav , Zherebtsov Dmitri , Romashov Mikhail , Selezneva Anastasia , Uchaev Daniil , Senin Anatoly , Chernukha Alexander
Published: September 2023

Adjusting the morphological characteristics of a material can result in improved electrocatalytic capabilities of the material itself. An example of this is the introduction of rare-earth elements into the borate structure, which gives a new perspective on the possibilities of this type of material in the field of (bio)sensing. In this paper, we present the preparation of borates including La, Nd and Dy and their application for the modification of a glassy carbon electrode, which is used for the non-enzymatic detection of a biologically relevant molecule, vitamin B6 (pyridoxine). Compared with the others, dysprosium borate has the best electrocatalytic performance, showing the highest current and the lowest impedance, respectively, as determined using cyclic voltammetry and impedance tests. Quantitative testing of B6 was performed in DPV mode in a Britton–Robinson buffer solution with a pH of 6 and an oxidation potential of about +0.8 V. The calibration graph for the evaluation of B6 has a linear range from 1 to 100 μM, with a correlation coefficient of 0.9985 and a detection limit of 0.051 μM. The DyBO3-modified electrode can be used repeatedly, retaining more than 90% of the initial signal level after six cycles. The satisfactory selectivity offered a potential practical application of the chosen method for the monitoring of pyridoxine in artificially prepared biological fluids with acceptable recovery. In light of all the obtained results, this paper shows an important approach for the successful design of electrocatalysts with tuned architecture and opens new strategies for the development of materials for the needs of electrochemical (bio)sensing.
Testing and Analysis of Surrounding Buildings during the Operation of Seismic Simulation Shaking Table
Authors: Xie Wenbo , Liang Huiqi , Zhang Zhiqiang , Wei Peizi , Lu Yijing
Published: September 2023

This paper focuses on the transmission of vibration response of the seismic simulation shaking table system at Southeast University, and analyzes the effect of this response on the surrounding building. The location of the building is less than 10 m away from the laboratory where the shaking table is located, and during the maximum load test, a student reported that the vibration caused him a sense of discomfort, as well as the shaking of office objects on desktops on high floors, which caused panic. For these reasons, multiple three-directional vibration sensors were placed around the shaking table system and on the 1st, 8th, and 16th floors of the building. Test the vibration response transfer and the vibration response of the building floor while the exercise table is in operation. The test results show that the vibration-weighted acceleration levels of some floors exceed the code limits under sinusoidal wave conditions, which was the cause of the discomfort. The floors will have an amplifying effect on the lateral vibration. For seismic wave excitation, all measurement points of the building meet the limit values required by the GB 50868-2013 Standard for allowable vibration of building engineering. This study can provide a data reference for future shaking table system commissioning and operation.
Characterization and Heat Transfer Assessment of CuO-Based Nanofluid Prepared through a Green Synthesis Process
Authors: Shanmugam Suresh Kumar , Arivendan Ajithram , Govindan Selvamani Samy , Dheivasigamani Thangaraju , Sundaresan Thirumalai Kumaran , Ali Saood
Published: September 2023

The manufacturing of copper oxide (CuO) nanoparticles has been accomplished utilizing a green technique that relies on biologically reliable mechanisms. Aqueous solutions of copper nitrate and Ixora Coccinea leaf extract are used in an environmentally safe process for creating CuO nanoparticles. The characterization of the synthesized CuO nanoparticles involves the utilization of techniques such as X-ray diffractometry (XRD), scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), and thermogravimetricanalysis (TGA). CuO nanoparticles are confirmed by XRD and FTIR peak results. When the particles are measured, they range between 93.75 nm and 98.16 nm, respectively. The produced CuO nanoparticles are used to prepare the nanofluid. While conventional water exhibits a 3 °C temperature difference, nanofluid achieves a considerable temperature differenceof 7 °C. As a result, it is clear that the nanofluid performs better at dispersing heat into the environment. The experiment’s overall findings support the possibility of ecologically friendly, green-synthesized CuO nanoparticle-induced nanofluid as an effective heattransfer fluid that can be applied to heattransfer systems.
Optimization of Plant Growth Regulators for In Vitro Mass Propagation of a Disease-Free ‘Shine Muscat’ Grapevine Cultivar
Authors: Kim Si-Hong , Zebro Mewuleddeg , Jang Dong-Cheol , Sim Jeong-Eun , Park Han-Kyeol , Kim Kyeong-Yeon , Bae Hyung-Min , Tilahun Shimeles , Park Sung-Min
Published: September 2023

This study addresses the propagation challenges faced by ‘Shine Muscat’, a newly introduced premium grapevine cultivar in South Korea, where multiple viral infections pose considerable economic loss. The primary objective was to establish a robust in vitro propagation method for producing disease-free grapes and to identify effective plant growth regulators to facilitate large-scale mass cultivation. After experimentation, 2.0 µM 6-benzyladenine (BA) exhibited superior shoot formation in the Murashige and Skoog medium compared with kinetin and thidiazuron. Conversely, α-naphthaleneacetic acid (NAA) hindered shoot growth and induced callus formation, while indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA) demonstrated favorable root formation, with IBA showing better results overall. Furthermore, inter simple sequence repeat analysis confirmed the genetic stability of in vitro-cultivated seedlings using 2.0 μM BA and 1.0 μM IBA, validating the suitability of the developed propagation method for generating disease-free ‘Shine Muscat’ grapes. These findings offer promising prospects for commercial grape cultivation, ensuring a consistent supply of healthy grapes in the market.