bluuebunny/crossref_metadata_2025 huggingface dataset:
Comparative transcriptomics analysis of contrasting varieties of Eucalyptus camaldulensis reveals wind resistance genes
Authors: Shang Xiuhua , Zhang Peijian , Liu Guo , Zhan Ni , Wu Zhihua
Published: February 2022

Background

Wind, an important abiotic stress factor, affects forests in coastal areas, causes tree damage and timber loss.

Methods

Two genotypes of Eucalyptus camaldulensis-strong wind-resistant CA5 and weak wind-resistant C037 were used for RNA-seq analysis to screen for candidate wind-resistance genes and transcription factors (TFs) by comparing the transcriptome analysis of the two varieties in response to wind stress.

Results

It showed that 7061 differentially expressed unigenes could be annotated including 4,110 up-regulated unigenes and 2,951 down-regulated unigenes. Gene Ontology (GO) analysis revealed that six cellulose pathways were involved in response to wind stress. The unigenes in phenylpropanoid biosynthesis, phenylalanine metabolism, and flavonoid biosynthesis pathways were found to be differentially expressed based on Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Moreover, 37 differentially expressed genes were functionally annotated to be involved in the secondary metabolism of phenylalanine (ko00940). Seventy-eight TFs related to the regulating cellulose and lignin synthesis were expressed differently from the various treatments. The expressions of C3H, POX, MYB, NAC, Gene008307, and Gene011799 were significantly upregulated in CA5. Overall, the main response of Eucalyptus to wind stress was associated with cell wall biosynthesis; key genes of cellulose and lignin biosynthesis pathways and related TFs were involved in the tree response to wind stress.
Turismo em territórios indígenas
Authors: Proença Ana Rosa Guimarães Bastos , Panosso Netto Alexandre
Published: February 2022

O turismo indígena no Brasil ocorre em diferentes territórios, e não somente nas que são denomina-das oficialmente pelo Estado Brasileiro como Terras Indígenas. Especificamente, na área rural de Manaus, Estado do Amazonas, dentro da Reserva de Desenvolvimento Sustentável Puranga Con-quista, a Comunidade Indígena Nova Esperança tem como prática o Turismo de Base Comunitária (TBC). Partindo do pressuposto de que os impactos socioculturais estão sob a luz do desenvolvi-mento do turismo, o objetivo desta pesquisa foi analisar como o modelo de turismo se relaciona com os impactos socioculturais no contexto da Comunidade Indígena Nova Esperança. A pesquisa tem abordagem qualitativa e foi desenvolvida com levantamento bibliográfico, documental e pesquisa de campo. Em campo, foram realizadas 21 entrevistas semiestruturadas, aplicados questionários socioeconômicos e observação participativa com inserção por dois meses consecutivos na comuni-dade. A análise qualitativa ocorreu por meio da triangulação dos dados. Os resultados apontam que o modelo de turismo em Nova Esperança avançou de uma prática de apenas receber o turista e esperar que ele comprasse o artesanato local, para um modelo de gestão interna que possibilitou a apropriação pelos indígenas do fluxo de turistas, do artesanato, da hospedagem e das demais ope-rações técnicas e financeiras. Sete impactos socioculturais principais foram identificados: benefícios econômicos; orgulho; relação com agentes intermediários; estilo de vida coletivo; comercialização da cultura;tradições e costumes; e, crenças. Foram identificados também impactos indiretos relaci-onados ao turismo
Intravesical Migration of the Longest Forgotten Intrauterine Contraceptive Device with Secondary Vesicolithiasis in a 70-Year-Old Female: A Rare Case Report
Authors: Mahajan Abhay Dinkar , Mahajan Sumeeta Abhay
Published: February 2022

Intrauterine Contraceptive Device (IUCD) is the most common method of contraception due to its low cost and reversibility. Longstanding IUCD’s can cause complications like menorrhagia, chronic pelvic pain, perforation of uterus and migration into adjacent structures as pouch of douglas, rectum or bladder. The Copper T acts as a foreign body and its presence for a long time causes secondary stone formation in bladder. Most of the cases in literature have been managed with open surgery. The author reports a case of a 70-year-old female presenting with transmigration of a forgotten Copper T into bladder with secondary calculus formation. The unique features of the presented case were the age of the patient, the longest duration of the forgotten Copper T (31 years) and the successful removal of both the calculi and Copper T device using an endoscopic approach.
Expansion to the Motion Sickness Susceptibility Questionnaire-Short Form: A Cross-Sectional Study
Authors: Ugur Emel , Konukseven Bahriye Ozlem , Topdag Murat , Cakmakci Mustafa Engin , Topdag Deniz Ozlem
Published: February 2022

Background and Objectives: The primary objective of this study is to investigate the necessity of questioning virtual reality systems in the motion sickness susceptibility questionnaire (MSSQ)-short form. The secondary objective of this study is to determine the validity and reliability of the Turkish version of the MSSQ-short form, with proven validity and reliability.Subjects and Methods: In the questionnaire form, for which expert opinion was obtained to maintain linguistic equivalence, the virtual reality items were added to the questionnaire. The questionnaire was then administered to 297 individuals. The results were statistically analyzed with and without these virtual reality items for validity and reliability.Results: After the addition of the virtual reality items, the reliability of the questionnaire was found to be quite high (Cronbach’s alpha r=0.912). The norm values between the original MSSQ-short form (12.9±9.9) and the Turkish MSSQ-short form (13.8±12.9) were found to be consistent.Conclusions: Motion sickness symptoms can occur not only during movement, but also with indirect stimulus. Our findings suggest that adding virtual reality items to the original form is important in long term practical applications. Our results show that the Turkish version of the original questionnaire is quite reliable. Submission of the MSSQ-short form in Turkish will be useful for documentation and will also encourage further research in this area.
CONCEPT OF NETRA RAKSHA IN AYURVEDA: A REVIEW
Authors: V Ajindas , Kumar K Pradeep
Published: February 2022

In the present scenario, the Netra Raksha concept is critical to keep up the health of the human eye. Nowadays, many eye problems are eliminated by avoiding faulty lifestyles and improper dietary habits. Eyes are vital to one’s quality of life. They're the windows to the soul, and yet the majority don't pay much respect to their care. The eyes usually get attention when there's a controversy, and one seek treatment for eye problems. We put our eyes through stress with the overuse of digital devices and exposure to an excessive amount of light. This is often hardly ever balanced out with adequate eye care practices. In Ayurveda Kriyakalpas like Seka, Aschyotana, Pindi, Vidalaka, Tarpana, Putapaka, Anjana for maintenance of eye health. And various Eye exercises, Dietary factors, simple procedures like Abhyanga, Snana, Nasya, Padabhyanga, Padaprakshalanam also are beneficial for the identical. So, a shot has been made to explore the strategies for preserving eye health.
Food Consumption and Determinants of Food Insecurity in the Department of Man (Western Côte d'Ivoire)
Authors: Yeo Kaseimibin , Gbakayoro Jean Brice , Yeo Lanzeni , Brou Kouakou
Published: February 2022

Despite the progress made in the framework of the Sustainable Development Goals (SDG) to ensure food security, pockets of food insecurity still remain in the Ivorian landscape. The objective of this study was to assess the food consumption of rural households in the department of Man in western Côte d'Ivoire and to identify the determinants of food insecurity. The assessment of food consumption was based on the calculation of the food consumption score. A survey of 1065 households was conducted to characterise the socio-economic profile and food consumption within these households. A survey of 1065 households was conducted to characterise the socio-economic profile and food consumption within these households. During the favourable period (October-February), 98.5% of households had an acceptable food consumption score, compared with 87.5% during the transitional period (March-June) and 15.5% during the lean season (July-September). The lean season is the most critical with 33.6% of households having a poor food consumption score and 50.9% of households having a borderline food consumption score. The results show that the gender and educational level of the head of household, the size of the household and the size of the crops influence food security. Food insecurity is experienced cyclically (every year) by households.
A Study on Farmer’s Knowledge on Pradhan Mantri Fasal Bima Yojana in Tumkur District of Karnataka
Authors: Nagesha G. , Moorthy Ganesh , Raju R. , Hani Umme
Published: February 2022

The current study was undertaken in Tumkur district of Karnataka. A total of 120 farmer beneficiaries of Pradhan Mantri Fasal Bima Yojana were selected by using the random sampling technique. About sixteen independent variables were selected based on judges' relevancy rating and were quantified using measurement devices. The data were collected using a pretested structured interview schedule and suitable statistical tools were used to analyze the data. The knowledge level of beneficiaries reported that, more than half of the farmers (53.33%) belongs to medium knowledge level, 25.00% belongs to high knowledge level and 21.67% belongs to low knowledge level. The variables like age, annual income, education, land holding, farming experience, extension contact, extension participation, scientific orientation, cosmopoliteness, achievement motivation, mass media exposure and crops cultivated had positive and significant association with knowledge level. Risk orientation, economic motivation and credit orientation had positive and significant at one per cent level. However, social participation was found to be non-significantly associated with the knowledge on Pradhan Mantri Fasal Bima Yojana. A comprehensive knowledge on scheme and an insurance of crops against natural disasters can become immense value to farmers. The government has to take new initiatives to help farmers thereby providing life security and make them confidence in their profession. Extension personnel working at gross root level should provide timely and complete information on Pradhan Mantri Fasal Bima Yojana by using suitable extension methods.
Malaria Infection among Under- Fives and Mothers: A Retrospective Study at Model Primary Health Centre, Ozuoba, Rivers State, Nigeria
Authors: Isaiah S. I. , Oparaocha E. T. , Nwaokoro J. C.
Published: February 2022

Malaria is a major public health problem and cause of suffering and premature death in tropical and subtropical countries. The population groups considered to be at a higher rate of contracting malaria and developing severe include infants, children under 5 years of age, pregnant women and patients with HIV/ AIDS, non-immune migrants, mobile populations and travelers. There is a need for constant research to be carried out to evaluate the prevalence. This study determined malaria infection among under-fives and mothers aged 8- 40 years that attended Model Primary Health Centre, Ozuoba, Rivers State, Nigeria between 2016- 2020. Records with medical diagnoses of all the children (0 – 5) years and mothers aged 18- 40 years that attended the health facility during the specified period were collected and analyzed. Data were analyzed with logistic regression, frequency, mean, standard deviations, and percentages using SPSS version 21.0 statistical software. The result of this study revealed an overall malaria infection rate of 46.7% for the under-fives and 35.4% for the mothers. Yearly infection rates of malaria among the studied groups were from 44% to 59%, within the five years studied. The highest infection rate (58.6%) for malaria in the under-fives occurred in 2016 and for the mothers, the highest (44.6%) was in 2019. Age was found to be a significant factor for malaria infection (p< 0.0001, χ2=434.3) among the studied under-fives. The ≥12 months old had less malaria infection rate (38.5%) while 48 months (4years) had the highest rate of infection (56.8%). Sex of the under-fives did not have significant impact on malaria infection rate (p = 0.205, χ2=1.606). Mothers’ age showed significant association with malaria infection (p = 0.006, χ2=12.597), with mothers 31 - 35years recording higher infection rate (37.7%) than those of 18-25 years old (30.8%). The education level of mothers also influenced infection rate (p = 0.0001, χ2=10.388) as those with tertiary education had the highest rate (38.9%) compared to those of secondary and primary levels at 36.8% and 36.7% respectively. The occupation of mothers was also found significant (p = 0.0001, χ2=25.585) with malaria infection being highest among farmers (44.4%), but lowest with students (29.7%).The results of this study showed a very high prevalence of malaria infection among the most vulnerable, and hence the need for improved access to all malaria interventional measures like quality and affordable malaria drugs and insecticide-treated nets.
Students’ Perception on Feedback Practice in Classroom to Enhance Learning
Authors: Nidup Dorji , Wangchuk Karma
Published: February 2022

This action research was intended to find out students’ perception of the practice of feedback in the classroom, its impact on their learning, their preference of modes of feedback, and the nature of feedback information and to improve the strategy of providing constructive feedback to students.

This action research was conducted at Lhuentse Higher Secondary School and engaged participants from seventh to ninth grade. The total sample population was 86 students. The study employed a quantitative approach to research. In this quantitative research design, predetermined statements with pre-coded five-point Likert scale questionnaires were self-administered to the participants to gather data.

The study revealed that constructive feedback has a positive bearing impact on the learning cycle of students. The study's findings revealed that descriptive feedback delivers a good amount of information, which ultimately helps students achieve learning goals.

Furthermore, the study showed that most students want feedback while performing a learning task, immediately after completing the learning task and on the quality of the task and skills. The findings also revealed that students want to practice peer assessment vigorously. Additionally, the findings showed that feedback should be made accessible to students since the choice of words and handwriting also determine the accessibility of students to get accurate information to improve themselves.
A Study of Etiopathogenesis of Different Vertigo Syndromes
Authors: Hande Vaidehi , Jain Shraddha
Published: February 2022

Background: Dizziness or disturbed postural awareness is common presentation in varying age groups. Dizziness can be classified into various types like Rotatory Vertigo, Imbalance/disequilibrium, Pre syncope/Light headedness and Psychogenic. Classically, there are four vertigo syndromes which are most commonly accepted among Otolaryngologists. However, cervicogenic vertigo is missing from the list of these vertigo syndromes. This study aims to assess the etiological factors for vestibular and central vertigo and other non-vestibular forms of vertigo and dizziness.

Methodology: This will be a prospective observational study conducted in the Department of Otorhinolaryngology, Jawaharlal Nehru Medical College, Wardha. About 75 patients presenting with symptoms of dizziness, tinnitus with associated nausea vomiting in the out-patient department will be included in this study. Patients will undergo clinical examination including Special Clinical Tests for vertigo like Dix-Hall pike Manoeuvre, Tandem walking and Dysdiadokokinesis. Collected data will be fed to appropriate statistical programme and analysed.

Expected Results: Relevant data on different etiopathogenic causes of vertigo will be obtained.

Conclusion: Better understanding of the various etiologies of various vertigo syndromes, their associations in various age groups and systemic diseases help better treatment outcomes.
Comparative Evaluation of Microleakage of Three Different Restorative Materials (Cention N, Zirconomer Improved and Glass Hybrid Restorative System) in Class V Cavity Restoration Using Stereomicroscope: In vitro Study
Authors: Dhivya S. , Vasanthakumari A. , Selvabalaji A. , Archana S. Preethi , Anagha C. , Kumar K.
Published: February 2022

Aim and Objective: Microleakage is the most important factor responsible for the destruction of restoration margins. The aim of this study was to compare and evaluate the microleakage of three different restorative materials namely glass hybrid restorative system, zirconomer improved and Cention N under stereomicroscope.

Materials and Methods: In this in-vitro experimental study, 45 Class V cavities were prepared on the buccal surface of maxillary premolars. The prepared cavities were divided into three groups for restoration with Cention N(group I), Zirconomer Improved(group II), Equiaforte (group III). All the samples were thermocycled for 500 cycles between 5-55°C initially and then immersed in 0.5% methylene blue for 24 hours before sectioning. All the sectioned samples were observed for microleakage under a stereomicroscope.

Results: Data was analyzed using one way ANOVA test for comparison between three groups and the result revealed statistically significant difference between the groups(p<0.05). Post hoc Tukey test was used for intergroup comparison and it was observed that both Cention and Equiaforte are inferior to Zirconomer improved in terms of microleakage (p<0.05).

Conclusion: Zirconomer improved exhibited lower microleakage when compared to Cention N and Equiaforte cements.
Genomics of Human Papillomavirus-induced Cervical Cancer
Authors: Umarvaish Shruti , Kumar Somendra , Kumar Anil
Published: February 2022

Human papillomavirus (HPV) induced cervical cancer is a serious health issue among most women from the least developed countries of the world due to scarcity of resources. HPVs have evolved a masterly infectious cycle that grabs the advantage of the self-renewal property of stratified cutaneous and mucosal epithelia. Firstly, the viral genome replicates episomally at a low copy number in the epithelial basal layers cells, with minimum viral transcription and translation. After that, when the infected cells are transmitted through the differentiation process maximum viral DNA synthesis and gene expression occur. The two major oncoproteins - E6 and E7, are responsible for inactivating the important tumor suppressor proteins, retinoblastoma (pRb) and p53. Due to the inactivation of these proteins, disruption occurs in the DNA replication, DNA repair mechanisms, oxidative induced damage (8-oxoguanine), aneuploidy, and apoptosis, leading to tumorigenesis. Hence, manipulation of E6 and E7 genes shows a successful result in the treatment of cervical cancer. The diagnosis tests include liquid-based preparations to improve the standard of the Pap smear; computer-based screening methods to improve Pap smear interpretation; and HPV testing methods that may be beneficial in triaging patients with untypical squamous cells of unknown significance or low grade squamous intraepithelial lesions (SILs). The continued studies of the molecular biology of HPVs are necessary to develop advanced screening techniques for testing and prophylactic vaccines for the elimination of HPV infection, also better therapeutic vaccines for the treatment of various HPVs infections.
Extended-spectrum β-lactamase Enzymes (ESBLs) and Slime Production of some Gram-negative Bacilli Isolated from Human, Animal and Environmental Sources in Port Harcourt, Nigeria
Authors: Horsfall S. J. , Nwokah E. G. , Wachukwu C. K. , Abbey S. D.
Published: February 2022

Aim: To determine extended-spectrum β-lactamase enzymes (ESBLS) and slime production of some Gram-negative Bacilli isolated from human, animal and environmental sources in Port Harcourt, Nigeria

Study design:  Cross-sectional study.

Place and Duration of Study: University of Port Harcourt Teaching Hospital, Trans-Amadi and Rumuodomaya Slaughters and Bob-D Ventures Poultry Farms, all in Port Harcourt, Nigeria, between July and December, 2019.

Methodology: This study was conducted at Port Harcourt and Obio/Akpor local government areas of Rivers State, Nigeria from 2019-2020. Hospital wastewater was taken from different sections at the two University Teaching Hospitals- University of Port Harcourt and Rivers State University Teaching Hospitals. Abattoir effluent water samples were taken at different sites from Trans-Amadi and Rumuodumaya Abattoirs. Chicken cloaca samples as well as Hand swab samples of Butchers were collected at the two Abattoirs. All samples were processed following standard procedures and identified organisms were assessed for susceptibilities to different antibiotics following Kirby-Bauer disk diffusion and Microbroth dilution methods. Isolates from the susceptibility testing with zone diameters found to be resistant to the Cephalosporins were suspected to be ESBL Positive (β-lactamase positive isolates). All isolates were also subjected to detection of slime production by Congo red agar plate method. Data were analyzed using SPSS version 22.0. Percentages and Chi square were used to summarize the data and p values less than 0.05 were considered significant.

Results: The results showed that out of the 224 isolates, 38 (17.0%) were ESBL-producing. ESBL-producers were more frequently isolated from Hospital Wastewaters (26.3%), followed by isolates from Poultry dung samples 12(24.0%) and Abattoir Effluent waters (15.0%). No ESBL-producing bacteria was recovered from the Butchers’ Hand swabs. Again, of the 38 ESBL-producing organisms, E. coli accounted for 24 (63.2%), followed by Klebsiella spp. 8 (21.1%), while Pseudomonas sp. recorded 6 (15.8%). Of the 224 isolates, 98 (43.8) were slime-producing, while 23 (60.5%) of the 38 ESBL-producing isolates produced slime.

Conclusion: ESBL and slime producing organisms (mostly E. coli) were more frequently isolated from Hospital Wastewaters compared to other sample areas.
A CIDADE SURREAL NA POÉTICA AFRO-BRASILEIRA CONTEMPORÂNEA: UM OLHAR À PRODUÇÃO DE CRIOLO
Authors: Andrade Lucas Toledo de
Published: February 2022

Este artigo pretende tratar da produção do músico e compositor Criolo como parte da poética afro-brasileira contemporânea, a partir da leitura de algumas composições e dos recursos formais trazidos por elas. O foco deste trabalho está na percepção do modo como a cidade é representada nas letras e ritmos propostos pelo artista e da forma como essa representação possibilita uma percepção expandida da existência, permitindo a reflexão sobre o discurso historiográfico brasileiro e a condição do negro no país, o que se relaciona diretamente com o potencial crítico e revolucionário presente na imagem surrealista.
NAS MÃOS DO ARTISTA, A CRIAÇÃO: A LITERATURA E O CINEMA NO ENSINO DO “DIFERENTE”
Authors: De Melo Weslei Chaleghi , De Santana Wilder Kleber Fernandes , Cordeiro Isabel Cristina
Published: February 2022

Neste estudo buscamos focalizar as múltiplas formas de expressão artística que compenetram a linguagem, relacionando a literatura e o cinema. Para tanto, desenvolvemos um diálogo que envolveu duas obras, uma literária (Elmer, o elefante xadrez) e outra cinematográfica (Dumbo). Procuramos apresentar brevemente a função social da arte e como ela pode ser inserida dentro de diversas discussões em sala de aula com enfoque na diversidade, trabalhando com conceitos de arte e literatura escola. Por meio da análise foi possível constatar que ambas as obras apresentam temas pedagógicos e literariamente relevantes para a reflexão aqui proposta, ou seja, diversidade e educação por meio da literatura e do cinema se constituem como recursos valiosos e que despertam nos alunos e nos professores sentimentos de alteridade.
OS INCÊNDIOS CRIMINOSOS SOB O OLHAR DOS ESQUECIDOS NO ROMANCE PALHA DE ARROZ, DE FONTES IBIAPINA
Authors: Silva Jéssica Maria Cruz , Lopes Maria Suely de Oliveira
Published: February 2022

O presente artigo analisa a obra Palha de Arroz, de Fontes Ibiapina (2004), tendo como foco a inter-relação entre ficção e história na construção de tal narrativa, que trata dos incêndios criminosos que aconteceram em bairros pobres de Teresina, capital do Piauí, durante a ditadura do Estado Novo. Escorado em autores como Costa Lima (1989), Pesavento (2006), Silva (2004), White (1994), que tratam da linha tênue entre literatura e história, bem como Lukács (2011) e Hutcheon (1991), no tocante às características do romance histórico, objetiva-se estabelecer uma reflexão crítica sobre o tema. Em linhas gerais, trata-se de um fato documentado, acrescido de outros recursos, próprios do novo romance histórico, como a ironia, a hipérbole, o grotesco, a reflexão em torno dos eventos narrados, construindo-se um texto em que história e literatura se relacionam.
CAMIÑANDO POLA LINGUA E A LITERATURA GALEGAS NA ATMOSFERA CONMEMORATIVA DO XACOBEO 2021-2022
Authors: Lopes Mailson , Luna Araceli
Published: February 2022

Por motivo do xacobeo compostelán (2021-2022) e tamén no clima do xubileu de prata da fundación do Centro de Estudos da Lingua e Cultura Galegas (CELGA), a coordinación e o lectorado deste centro de estudos galegos situado na Universidade Federal da Bahia (UFBA) ofrecen ao público un número especial da Revista Estudos Linguísticos e Literarios dedicado á lingua e á literatura galegas. Após a coidadosa avaliación por parte de especialistas da área, chegouse a un conxunto de dezanove textos, da pluma de vinte e sete investigadores, provenientes de diversas rexións de Brasil e de Galicia — mais tamén de outros territorios, como Hungría e Estados Unidos—, que dan corpo a este volume, publicado como un marco festivo e duradeiro das tan significativas conmemoracións mencionadas.
DOS PUTOS DE PERO DA PONTE: NOTAS PARA A HISTÓRIA DE UMA PALAVRA
Authors: Sacramento de Souza Arivaldo
Published: February 2022

A sátira medieval galego-portuguesa é fonte diversa para compreensão do significado social das relações entre homens. Embora não seja o corpus em que possamos ouvir a voz do “sodomita”, é uma vitrine ampla para o entendimento dos discursos contra a sodomia. Nessa perspectiva, tomamos a cantiga de Pero da Ponte, [Eu digo mal com’ome fodimalho] para analisar os sentidos da palavra “puto”, única ocorrência no masculino em todos os Cancioneiros. Para isso, acionamos um conjunto de elementos da prática filológica (paratextos, fontes indiretas, análise de obras lexicográficas e de edição) que possibilitaram encontrar alguns traços semânticos que nos ajudaram a entender a mudança de sentido e a polissemia do termo. Dessa investigação, pudemos chegar às seguintes questões: “puto”, na ocorrência da cantiga de Pero da Ponte, é hiperônimo para as relações sexuais entre homens (ativo ou passivo); a mudança em direção à ampliação dos sentidos sexuais da palavra “puto” aconteceu desde a Antiguidade e generaliza-se numa dimensão moral na Idade Média; “puto” parece ter mudado por meio da relação entre mocidade e pureza moral, dessa síntese emergiu a coisificação desses corpos também em contextos de prostituição.
ESCRITA E VISUALIDADE EM POESIA ÚLTIMA DE AMOR E ENFERMIDADE DE LOIS PEREIRO
Authors: Mello Marcelo Cordeiro de
Published: February 2022

A proposta deste artigo é discutir a relação entre escrita e visualidade no livro Poesia última de amor e enfermidade do poeta galego Lois Pereiro, especialmente em quatro poemas, cujas traduções para o português são apresentadas como anexo. Transitamos entre autores que pensaram o problema do objeto-livro e da iconicidade da escrita, tais como: Roland Barthes, Michel Butor, Anne-Marie Christin, Georges Didi-Huberman, Gotthold Lessing, Stephane Mallarmé, Michel Melot, Georges Perec e Denis Roche. Por meio do pensamento destes autores, discutimos o problema da organização deste livro de Lois Pereiro, a partir do problema da página dupla e do diálogo intertextual que ela estabelece.
Suggesting an Appropriate Angle for Installation of Bicycle Headlight
Authors: Kim Yerim , Joo Jaeseung , Jung Dojoon
Published: February 2022

In this study, we experimentally investigated the change in the degree of glare disturbance with respect to the installation angle of bicycle headlights. In particular, we aimed to determine the optimal headlight angle that resulted in the least glare from the bicycle driver while securing a visible distance that ensured driver safety. The test subject was riding a bicycle and was requested to look at a bicycle equipped with a headlight facing the approaching direction. Additionally, the angle between the headlight and the ground was changed from 0° to 30° in the downward direction, as the subject was requested to drive repeatedly. The subject's gaze was tracked using an eye tracker to measure the time spent gazing at the headlight. The results were then utilized to evaluate the degree of glare disorder.
Analysis of an Evacuation Simulation for the Development of Scenarios for Fire Evacuation Support Devices
Authors: Choi Doo-Chan , Hwang Hyun-Su , Ko Min-Hyeok
Published: February 2022

In this study, an evacuation simulation was performed to analyze the appropriate number of robots and occupants, which are major parameters required to develop a fire evacuation scenario for an evacuation device. When a horizontal evacuation aid robot was used, the evacuation time decreased and later increased with increasing number of robots. Further, the time determined by the space of the nursing home and the number of occupants. To properly utilize the horizontal evacuation device, it is assumed that the path of the robot is closed and that the fire brigade can enter through the window and stairs.
Flushing Effect Depending on the Installation Density and Opening Order of Fire Hydrants in Water Supply Networks
Authors: Hong Sungjin , Joo Jin Gul , Lee Sooho , Yoo Do Guen
Published: February 2022

In this study, using the WaterGEMS model, the hydrant installation density in small blocks and the flushing effect according to the opening order were analyzed for various scenarios and the results were compared. The analysis results revealed that even in the case of conventional flushing, the same flushing could be achieved by reducing the number of openings by up to 20% (30 → 24) when the optimal opening order was determined through preliminary simulation, rather than simply opening all hydrants at random. I knew it could work. In addition, it was confirmed that as the density of hydrants increased, the effect satisfaction extension through conventional flushing increased. In particular, when the number of hydrants installed in the target area was approximately 30, the flushing effect was confirmed to be achieved in most pipe extensions. The proposed methodology can be used to establish a detailed implementation plan for flushing in the detailed standards for the maintenance of waterworks pipe network facilities that mandate flushing for the water supply network.
Behavioral Characteristics of a Single Pile According to the Relative Density of Two-Layered Soil during Lateral Loads
Authors: Kim Jiseong , Kang Gichun
Published: February 2022

This study attempts to understand the behavioral characteristics of a pile in two-layered soil through model tests. A lateral load was applied to a single pile buried in the ground with different relative densities and strata ratios, and the lateral resistance and bending moment of the pile were analyzed. Consequently, when the strata ratio was 0.15, there was a clear difference in the values of the lateral resistance and the coefficient of the subgrade reaction of the pile according to the relative density of the lower ground. When the strata ratio was 0.30 or more, it was observed that the values of the lateral resistance and the coefficient of the subgrade reaction of the pile did not significantly affect the relative density of the lower ground. The locations of the maximum bending moments appeared in increasingly deeper places as the strata ratio increased, and all occurred in the lower ground with a high relative density.
Analysis of Operation Status and Effectiveness of Activities through Community Mapping
Authors: Lee JongHoon , Park SangHyun , Heo Boyoung
Published: February 2022

Local residents can use community mapping to identify local issues and participate in decision-making to establish development plans. Administrative blind spots can be minimized as local residents directly collect data within their living environments. Therefore, community mapping can promote various changes in the community by connecting individuals, organizations, and regions. The National Disaster Management Research Institute has been supporting institutions in promoting safe community mapping activities since 2016. According to the results of the community mapping survey for the analysis of change in safety consciousness, participants were able to strengthen their safety awareness and increase the effectiveness of safety-culture activities using community mapping. Based upon these effects, we expect to continue to operate community mapping to solve local problems and improve the community environment.
Study on Extinguishing Limits of Liquid Fuel Flames in Confined Spaces
Authors: Jeon Kilsong , Sim Jaeung , Hwang Jihyun , Kim Dohyun , Lee Taewon
Published: February 2022

To sustain a flame following fuel ignition, it must receive oxygen from the surroundings at a concentration above a stoichiometrically-determined threshold; otherwise, the flame will decrease in intensity or extinguish. These thresholds vary depending on the type of fuel. Using the aforementioned factors, it is possible to predict and control the characteristics of flames in confined spaces. While the combustion characteristics of flames exposed to the atmosphere have been actively studied under controlled conditions, it is more challenging to predict and remediate the impact of variables, which manifest in real fire sites. In this study, following the ignition of a flame in a confined space with limited oxygen supply, oxygen and carbon dioxide levels and temperature at this space were measured according to changes in the fuel supply capacity. The extinguishing limit oxygen concentration as well as the extinguishing time decreased in relation to the size of the initial source. These results can guide the design and control of fire-fighting equipment for the early detection and extinguishing of fires.
Measures to Improve Safety Management of Small-Scale Hazardous Substance Factories
Authors: Kwon Hyeogsic , Kwak Dae-Hoon , Ko Wangyoul
Published: February 2022

Although fires in plants that manufacture hazardous materials do not occur frequently, the damages caused in a single such event are relatively large. Thus, it is essential to establish a safety management system for such factories. The primary aim of this study is to propose the improvement measures based on the analysis of standards according to the Dangerous Substances Safety Management Act, fire site surveys, and complete inspection of small-scale factories manufacturing dangerous substances. The specifications of the open holding area should be greater in number than the designated quantity; for example, the lightning rods should be mandatorily installed in a quantity of 10 times or more than the designated one. Other suggested measures include mandatory preparation of preventive regulations and regular inspection of all manufacturing plants by an external safety management agency, mandatory reporting and on-the-job training for substitutes of hazardous substance safety managers, and conducting a minimum qualification test for the post of safety manager and ensure that only those who have passed the test are employed. The existing laws and regulations should be amended to ensure that accidents can be prevented through systematic safety management.
A peculiar repetitive sequence in the rat genome
Authors: Nojima Hiroshi , Sokabe Hirofumi
Published: July 2002

We report here a new type of peculiar repetitive sequence, A15T(TC)9T12, which was detected at 750 base pairs (bp) upstream of a rat calmodulin processed pseudogene by DNA sequencing of cloned DNA fragments. This sequence element could possibly form a cruciform structure with a 12‐AT‐pair stem, exposing (CT)9 sequences as a loop. S1 nuclease protection experiments failed to identify this element as a cruciform structure but instead detected an alternating purine pyrimidine tract at 50 bp downstream of this element. Total genomic Southern blotting showed that the rat genome contains only a few of these elements.
Influence of the hydrophobicity of lipase isoenzymes from Candida rugosa on its hydrolytic activity in reverse micelles
Authors: Otero Cristina , Rúa M.Luisa , Robledo Laura
Published: July 2002

Two isoenzymes of Candida rugosa lipase, having the same mol.wt., size and similar aminoacid sequence, were studied in reverse micelles of AOT. The results demonstrated the relevance of lipase hydrophobicity in reactions in anionic micelles. This is a key factor in mitigating the inhibition effect of charged micelles. The more hydrophobic isolipase A was a better biocatalyst for hydrolytic processes in these systems. Its α‐helix content increased from 31% to 49% of the total structure in reverse micelles. A fluorescence study indicated a more apolar environment for the more hydrophobic isolipase A. Emission spectra of this isolipase in the AOT systems were blue shifted. At ω
0 values where each isolipase presented its maximum activity, a decrease of the emission intensity of Trp was found. An enzyme and substrate dependence of optimal ω

o
is reported. The different interaction of isolipases A and B with the micellar system produced an opposite ω
0 dependence to their stabilities. The more hydrophobic lipase A had higher stability at higher droplet sizes.
Transcription factor‐mediated chromatin remodelling: mechanisms and models
Authors: Varga-Weisz Patrick D. , Becker Peter B.
Published: July 2002

The association of DNA with nucleosomes in chromatin severely restricts the access of the regulatory factors that bring about transcription. In vivo active promoters are characterised by altered, almost transparent chromatin structures that allow the interaction of the transcriptional machinery. Recently, enzymatic activities have been discovered that facilitate the binding of transcription factors to chromatin by modifying nucleosomal structures in a process that requires energy. The mechanisms by which chromatin is remodelled may involve nucleosome movements, their transient unfolding, their partial or even complete disassembly. The dynamic properties of chromatin that underlie these structural changes are fundamental to the process of regulated gene expression.
Oxygen equilibrium and electron paramagnetic resonance studies on copper(II)‐iron(II) hybrid hemoglobins at room temperature
Authors: Shibayama Naoya , Ikeda-Saito Masao , Hori Hiroshi , Itaroku Kazuya , Morimoto Hideki , Saigo Satoshi
Published: July 2002

Copper(II_‐iron(II) hybrid hemoglobins, in which hemes in either the α or β subunits are substituted with copper (II) protoporphyrin IX, have been prepared. The affinities of the ferrous‐subunits in both hybrids for the first binding oxygen are as low as the affinity of deoxyhemoglobin under various solution conditions, indicating the equality of behavior in copper(II) protoporphyrin IX and deoxyheme. Electron paramagnetic resonance (EPR) examinations on these hybrids at room temperature show that the interaction between copper(II) and the proximal histidine (F8) is specifically weakened in the α subunits within a low affinity conformation of hemoglobin. These results suggest that copper(II) protoporphyrin IX is a useful EPR probe at room temperature for investigating the deoxyheme environment in hemoglobin.
Dose‐dependent activation and block by bisG10, a K+ channel blocker, of mouse and frog skeletal muscle KATP channels
Authors: Allard Bruno , Fournet Guy , Rougier Oger , Descans Bérangère , Vivaudou Michel
Published: July 2002

The effects of a K+ channel blocker, bisG10, were examined on ATP‐sensitive K+ (KATP) channels in membrane patches excised from mammalian and amphibian skeletal muscle fibres using the patch‐clamp technique. At micromolar concentrations, bisG10, added on the intracellular side, induced a strong, reversible, flickery block of KATP channels. BisG10, added on the extracellular side, was about 100‐fold less potent at inhibiting channel activity. At 10 nM, intracellular bisG10 increased KATP channel activity. This activation was independent of the presence of internal ATP or Mg2+. The inhibitory effect of bisG10 most likely arose from open‐channel block whereas activation could result from more complex, indirect interactions.
Transcriptional regulation of apolipoprotein A‐I expression in Hep G2 cells by phorbol ester
Authors: Vandenbrouck Yves , Lambert Gilles , Janvier Brigitte , Girlich Delphine , Bereziat Gilbert , Mangeney-Andreani Marise
Published: July 2002

The regulation of apolipoprotein A‐I (apo A‐I) gene expression by 12‐O‐tetradecanoylphorbol 13‐acetate (TPA) was investigated in the human hepatoma cell line Hep G2. TPA treatment decreased apo A‐I mRNA levels in a time‐dependent manner, by up to 50% versus control cells within 24 h. Nuclear run‐on transcription assays demonstrated a transcriptional effect of TPA. Using transfection analysis with a plasmid construct containing the −1378/+11 apo A‐I promoter fused to the secreted placental alkaline phosphatase (SPAP) reporter gene, we showed that the SPAP activity was decreased to 50% when Hep G2 cells were incubated in the presence of TPA. The inhibitory effect of TPA was still maintained when fragment −253 to −4 of apo A‐I promoter was linked to the CAT reporter gene. These data indicate that transcriptional modulation of apolipoprotein A‐I gene expression following phorbol ester treatment is transduced by gene elements located between −253 and −4 of the apo A‐I promoter.
Endometrial müllerian carcinosarcoma after cessation of tamoxifen therapy for breast cancer
Authors: Sasco A.J. , Raffi F. , Satgé D. , Goburdhun J. , Fallouh B. , Leduc B.
Published: July 2002

The aim of this report is to draw the attention of clinicians to the possible occurrence of endometrial cancers of rare histological type among women currently undergoing or having in the past undergone tamoxifen therapy, in particular for breast cancer. We report a case of heterologous mixed malignant müllerian tumor occurring in an 80‐year‐old woman. At 69, she had been diagnosed with breast cancer and received tamoxifen for a total of 55 months over a 6‐year period. In the 5th year after cessation of tamoxifen therapy, an endometrial carcinosarcoma was diagnosed. Although the association between tamoxifen use and endometrial cancer is recognized, only a few reports of occurrence long after cessation of therapy exist. We believe ours is the second for this particular histological type. Tamoxifen may have played a role in the occurrence of this tumor although it is also known that this type of tumor may arise de novo in elderly women. The etiologic hypothesis obtained from this case description will now be tested in a formal epidemiological investigation which hopefully will provide more definitive evidence.
Balloon valvuloplasty during pregnancy
Authors: Onderoglu L. , Tuncer Z.S. , Oto A. , Durukan T.
Published: July 2002

Although rheumatic diseases are reported to be almost eradicated in the developed countries, they still continue to contribute significantly to maternal mortality in the developing world. Surgical therapies need to be considered for those patients who do not respond satisfactorily to medication. However, valve replacement or valvulotomy during pregnancy carries significant risks for both the mother and the fetus. Two patients with severe mitral stenosis refractory to medical therapy are presented. Both patients were subjected to percutaneous balloon valvuloplasty during pregnancy without any complications. They were able to discontinue medication and deliver vaginally at term. Balloon valvuloplasty appears to be a safe alternative to conventional surgical approaches in pregnancy.
Fetal biophysical profile and vibratory acoustic stimulation in high‐risk pregnancies
Authors: Petrović O. , Frković A. , Matejčić N.
Published: July 2002

Objectives: To determine the influence of the non‐stress test (NST) on the efficiency of the fetal biophysical profile (FBP) and to test the clinical usefulness of the FBP and its combination with vibratory acoustic stimulation (VAS) in managing high‐risk pregnancies. Methods: One hundred twenty fetuses of preeclamptic patients were included in a prospective study. Five standard variables of the FBP were observed ultrasonically following NST. In cases of non‐reactive NST, external VAS was applied and the FBP score calculated and compared with the FBP score before VAS. Results: Of 120 calculated FBPs, 102 (85%) had normal profile scores before VAS and 104 (86.7%) after VAS. No statistically significant difference was found. The sensitivity, specificity, and positive and negative predictive values of the FBP score in predicting poor perinatal outcome were 94.7%, 94.4%, 75% and 99%, respectively. VAS produced a high conversion (58.8%) of non‐reactive NST to reactive fetal heart rate pattern. The false‐negative rate of the FBP score was 9.8 per 1000, which did not increase after VAS. Conclusions: The efficiency of the FBP score was not significantly improved by VAS, although a high conversion of non‐reactive to reactive NST was produced. The FBP with its three ‘acute biophysical variables’ was found to be an accurate method of antepartum assessment even without an NST. The low incidence of perinatal complications among patients with normal FBP scores, permits the conservative management of preterm high‐risk pregnancies.
Identification of the regions involved in DNA binding by the mouse PEBP2α protein
Authors: Pérez-Alvarado Gabriela C. , Munnerlyn Audrey , Dyson H.Jane , Grosschedl Rudolf , Wright Peter E.
Published: July 2002

The polyomavirus enhancer binding protein 2α (PEBP2α) is a DNA binding transcriptional regulatory protein that binds conserved sites in the polyomavirus enhancer, mammalian type C retroviral enhancers and T‐cell receptor gene enhancers. Binding of PEBP2α and homologous proteins to the consensus DNA sequence TGPyGGTPy is mediated through a protein domain known as the runt domain. Although recent NMR studies of DNA‐bound forms of the runt domain have shown an immunoglobulin‐like (Ig) fold, the identification of residues of the protein that are involved in DNA binding has been obscured by the low solubility of the runt domain. Constructs of the mouse PEBP2αA1 gene were generated with N‐ and C‐terminal extensions beyond the runt homology region. The construct containing residues Asp90 to Lys225 of the sequence (PEBP2α90–225) yielded soluble protein. The residues that participate in DNA binding were determined by comparing the NMR spectra of free and DNA‐bound PEBP2α90–225. Analysis of the changes in the NMR spectra of the two forms of the protein by chemical shift deviation mapping allowed the unambiguous determination of the regions that are responsible for specific DNA recognition by PEBP2α. Five regions in PEBP2α90–225 that are localized at one end of the β‐barrel were found to interact with DNA, similar to the DNA binding interactions of other Ig fold proteins.