bluuebunny/crossref_metadata_2025 huggingface dataset:
A Comprehensive Review of Catheter-Related Thrombosis
Authors: López-Rubio Marina , Lago-Rodríguez Marta-Olimpia , Ordieres-Ortega Lucía , Oblitas Crhistian-Mario , Moragón-Ledesma Sergio , Alonso-Beato Rubén , Alvarez-Sala-Walther Luis-Antonio , Galeano-Valle Francisco
Published: December 2024

Catheter-related thrombosis (CRT) is a frequent and potentially serious complication associated with the widespread use of intravascular devices such as central venous catheters, including peripherally inserted central catheters and implantable port systems, pacemakers or implantable cardioverter-defibrillators. Although CRT management has been informed by guidelines extrapolated from lower extremity deep vein thrombosis (DVT), unique challenges remain due to the distinct anatomical, pathophysiological, and clinical characteristics of upper extremity DVT. Risk factors for CRT are multifactorial, encompassing patient-related characteristics such as cancer, prior venous thromboembolism, and infection, as well as catheter-specific factors like device type, lumens, and insertion site. The diagnosis of CRT relies primarily on ultrasonography; however, computed tomography angiography and magnetic resonance imaging play a complementary role, particularly in anatomically challenging cases or when complications such as pulmonary embolism or superior vena cava syndrome are suspected. Treatment strategies for CRT include anticoagulation, catheter removal when feasible, and, in select cases, local thrombolysis or catheter-directed interventions. Anticoagulation remains the cornerstone of therapy, with direct oral anticoagulants increasingly favored due to their safety profile and efficacy. This article provides a detailed review of CRT, focusing on clinical features, diagnostic methods, and treatment strategies while addressing specific challenges in managing pacemaker and hemodialysis catheter-related thrombosis.
Nd:Yag Laser Transluminal Embolysis: A Therapeutic Approach in Retinal Artery Occlusion
Authors: Tăbăcaru Bogdana , Abboud George , Munteanu Mihnea , Stanca Simona , Stanca Horia Tudor
Published: December 2024

Background: Central and branch retinal artery occlusion (CRAO and BRAO) are critical causes of acute vision loss, predominantly affecting older adults with systemic vascular pathology. These occlusions typically result from embolic events, leading to partial or complete retinal ischemia. Methods: This retrospective case series report details of our 10-year experience using the 1064 nm Nd:YAG laser for Transluminal Nd:YAG Embolysis (TYE) in order to lyse visible emboli within the retinal arteries. Results: We conducted a retrospective case series involving 18 patients treated by TYE for different retinal arteries occlusions between 2014 and 2024. TYE effectively restored blood flow in the occluded arteries, with variable but generally favorable visual outcomes. Our article highlights significant clinical and interventional aspects of three treated patients: a BRAO case with multiple transluminal emboli, a case of double BRAO and a CRAO case. We also report the successful use of TYE for intraluminal lysing of an embolus located outside the optic disc. The procedures were well-tolerated, with minor complications such as preretinal or vitreous hemorrhages resolving spontaneously. Conclusions: TYE is a minimally invasive therapeutic option for retinal artery occlusion with visible emboli, particularly when intervention occurs shortly after the onset of symptoms. Compared to observation, TYE can improve visual outcomes and reduce the risk of permanent vision loss, presenting a significant advancement in the management of this ophthalmic emergency. The worldwide experience with TYE is continuously increasing. Further research with larger cohorts is recommended to validate these results and refine the treatment protocol.
Exercise as a Therapeutic Strategy to Improve Cerebrovascular Function and Cognition in Breast Cancer Survivors: A Scoping Review
Authors: Downs Tahnee L. , Whiteside Eliza J. , Denham Joshua , Mills Dean E. , Bliss Edward S.
Published: December 2024

Breast cancer is the most diagnosed cancer globally. While the breast cancer prevalence continues to rise, so too do patient survival rates, thus resulting in a large survivor population. Up to 75% of this population report experiencing cancer-related cognitive impairment during their cancer journey, thus reducing their quality of survivorship. This review systematically evaluates the effect of physical activity and exercise training on cerebrovascular function and cognition in breast cancer survivors. Cross-sectional, intervention or observational studies that examined the effect of acute or chronic exercise training or physical activity levels on cerebrovascular function and cognition in female breast cancer survivors were searched for systematically. The 11 included studies were tabulated and described narratively. The included studies primarily focused on aerobic exercise training, while only four studies investigated the effect of resistance exercise training or concurrent training on cerebrovascular function and/or cognition in breast cancer survivors. Collectively, these studies provide preliminary evidence supporting the positive effect of exercise training on cerebrovascular function and cognition in breast cancer survivors, irrespective of their age, stage of breast cancer and treatment regimen. However, more research is required to comprehensively evaluate the effect of exercise training on cerebrovascular function and cognition in breast cancer survivors and the mechanisms leading to these potential improvements.
A Prospective Study on the Feasibility and Effect of an Optimized Perioperative Care Protocol in Pediatric Neuromuscular Scoliosis Surgery
Authors: Naume Marie Mostue , Hoei-Hansen Christina Engel , Born Alfred Peter , Brekke Ghita , Høj Astrid , Nielsen Maja Risager , Borgwardt Lise , Vissing John , Dirks Jesper , Rye Anne Kathrine Stæhr , Møller Morten Hylander , Andersen Thomas Borbjerg , Ørngreen Mette Cathrine
Published: December 2024

Background/Objectives: A recent retrospective study conducted by our team identified a high percentage of postoperative pneumonia in children with neuromuscular scoliosis. Based on the findings in that study and our clinical experience, we aimed to assess the effectiveness of an optimized perioperative care protocol. Methods: As part of a prospective study, a multidisciplinary team developed a protocol that included preoperative nutritional and respiratory optimization, intra- and postoperative intravenous glucose infusion, early extubation, and postoperative nutritional optimization. Non-ambulant children between 6 and 18 years of age with neuromuscular scoliosis were eligible for inclusion in the study. The primary outcome was the rate of postoperative pneumonia within 30 days of surgery. The secondary outcome measures were the rate of postoperative complications, including readmissions. All the outcomes were compared to a retrospective control group that was receiving standard care during the same period. Results: Eleven children were included in the intervention group and 14 in the control group. In regard to the intervention group, the nutritional and respiratory assessment before surgery resulted in optimized treatment in 8/11 patients (73%) and 9/11 patients (82%), respectively. One patient (9%) in the intervention group and three patients (21%) in the control group developed postoperative pneumonia (relative risk 0.42, 95% confidence interval 0.05–3.50). The intervention and control groups did not differ significantly in terms of postoperative complications or readmission rates. Conclusions: The multidisciplinary care protocol is feasible, with a high compliance rate in regard to study procedures. A numerical reduction in the 30-day pneumonia rate did occur in the intervention group; however, this reduction did not reach statistical significance.
Minimally Invasive Surgery in Acute Bowel Obstruction: Should It Become the Standard of Care? A Prospective, Single Center, Observational Study
Authors: Hoi Hannes , Grünbart Martin , de Cillia Michael , Uzel Robert , Hofer Hannah , Schlosser Lisa , Tschann Peter , Weiss Helmut , Mittermair Christof
Published: December 2024

Background/Objectives: This study was conducted to determine whether a structured clinical pathway can help to safely implement minimally invasive surgery (MIS) as the standard approach in surgery for acute bowel obstruction (ABO). Methods: A prospective analysis was performed on consecutive patients undergoing MIS for ABO at a single center in 2021 and 2022. Prior to the study onset, a structured treatment pathway was defined to apply MIS in all patients. The rate of success in the MIS and patient-, surgeon- and outcome-specific parameters with a focus on complication and conversion rates were analyzed. Results: Compared to a historical control group, the conversion rate from MIS to open surgery (OS) nearly doubled from 20.4% to 36.4% (p = 0.14). The complication rate in converted patients was 43.8% vs. 7.15% in non-converted patients (p < 0.05). Conclusions: A structured clinical pathway, including technical standardization and preoperative decisional processes, can be used to implement MIS as a primary surgical treatment in ABO. This is accompanied by high conversion rates and a significant increase in postoperative complications in patients undergoing conversion. Individual decision-making concerning the surgical approach remains paramount to prevent complications and high conversion rates.
Prognostic Role of Lymphocyte-to-C-Reactive Protein Ratio in Patients with Pulmonary Arterial Hypertension
Authors: Chen Meng-Qi , Wan Chuan-Xue , Tong Jun , Wang An , Ruan Bin-Qian , Shen Jie-Yan
Published: December 2024

Background: Inflammation plays a critical role in the prognosis of patients with pulmonary arterial hypertension (PAH). The lymphocyte-to-C-reactive protein ratio (LCR), as a novel inflammatory marker, has not been studied in patients with PAH. The objective of this study was to investigate the prognostic value of the LCR in patients with PAH. Methods: A retrospective cohort study was conducted on 116 patients with PAH diagnosed in Renji Hospital, School of Medicine, Shanghai Jiao Tong University, from January 2014 to December 2018. The primary outcome was a composite endpoint that included lung transplantation, rehospitalization for PAH, and all-cause death. The LCR is the ratio of the blood lymphocyte count to the C-reactive protein concentration. Results: A total of 116 patients with PAH were included in this study, with an average age of 41.53 years; 92.2% were female, and the event rate was 57.8%. Restricted cubic spline analysis confirmed a linear association between the LCR and the risk of clinical worsening events. Multivariate Cox proportional hazards analysis showed that the LCR was significantly negatively associated with clinical worsening events, with hazard ratios and 95% confidence intervals of 0.772 (0.614–0.970). The Kaplan–Meier curve showed that event-free survival decreased significantly when the LCR was less than 1.477. LASSO regression selected four potential predictors, including the LCR, to construct a nomogram. The nomogram had a high predictive strength, with an area under the ROC curve of 0.805 (0.713–0.896). The calibration curves and decision curve analysis indicated that the nomogram had good predictive performance and the ability to guide clinical management. Conclusions: The LCR is a valuable prognostic marker for predicting long-term clinical events in patients with PAH, and the nomogram incorporating the LCR could effectively stratify risk and guide clinical decision making.
Digital Twins Use in Plastic Surgery: A Systematic Review
Authors: Seth Ishith , Lim Bryan , Lu Phil Y. J. , Xie Yi , Cuomo Roberto , Ng Sally Kiu-Huen , Rozen Warren M. , Sofiadellis Foti
Published: December 2024

Background/Objectives: Digital twin technology, initially developed for engineering and manufacturing, has entered healthcare. In plastic surgery, digital twins (DTs) have the potential to enhance surgical precision, personalise treatment plans, and improve patient outcomes. This systematic review aims to explore the current use of DTs in plastic surgery and evaluate their effectiveness, challenges, and future potential. Methods: A systematic review was conducted by searching PubMed, Scopus, Web of Science, and Embase databases from their infinity to October 2024. The search included terms related to digital twins and plastic surgery. Studies were included if they focused on applying DTs in reconstructive or cosmetic plastic surgery. Data extraction focused on study characteristics, technological aspects, outcomes, and limitations. Results: After 110 studies were selected for screening, 9 studies met the inclusion criteria, covering various areas of plastic surgery, such as breast reconstruction, craniofacial surgery, and microsurgery. DTs were primarily used in preoperative planning and intraoperative guidance, with reported improvements in surgical precision, complication rates, and patient satisfaction. However, challenges such as high costs, technical complexity, and the need for advanced imaging and computational tools were frequently noted. Limited research exists on using DTs in postoperative care and real-time monitoring. Conclusions: This systematic review highlights the potential of digital twins to revolutionise plastic surgery by providing personalised and precise surgical approaches. However, barriers such as cost, complexity, and ethical concerns must be addressed. Future research should focus on validating clinical outcomes through large-scale studies and developing soft tissue modelling and real-time monitoring capabilities.
Pregnancy Outcomes in Non-Criteria Obstetric Antiphospholipid Syndrome: Analysis of a Cohort of 91 Patients
Authors: Beça Sara , Baños Núria , Borrell Maria , Ruiz-Ortiz Estíbaliz , Pérez-Isidro Albert , Cervera Ricard , Reverter Joan Carles , Tàssies Dolores , Espinosa Gerard
Published: December 2024

Background: The clinical and laboratory features of patients with non-criteria obstetric antiphospholipid syndrome (NC-OAPS), as well as their pregnancy outcomes and ideal treatment are not clearly determined. The aim of this study is to describe the characteristics and outcomes of pregnancies in NC-OAPS and compare them with an obstetric APS (OAPS) cohort. Methods: This is a retrospective study conducted on a cohort of women referred to a high-risk obstetric unit of a tertiary hospital. Women that were classified as having OAPS or NC-OAPS were included and compared in terms of clinical and laboratory characteristics, management, and subsequent pregnancy outcomes. Results: We identified 107 women with 143 pregnancies, 91 with NC-OAPS and 16 with OAPS. There were no differences in demographic features between both groups. Women with NC-OAPS were more likely to have recurrent implantation failure and were predominantly positive for a single antiphospholipid antibody (aPL) subtype. Both groups were treated similarly (low dose aspirin plus low molecular weight heparin in 87.4% of NC-OAPS and 83.3% of OAPS, p > 0.05). Live birth rate (82.4% and 75.0%, respectively, p > 0.05) and adverse pregnancy outcomes (31.6% vs. 37.5%, p > 0.05) in subsequent pregnancies during follow-up were also similar between groups. Conclusions: This study revealed differences in the previous pregnancy morbidity and aPL profiles in women with NC-OAPS and OAPS, although the therapeutic approach and the outcomes of subsequent pregnancies were similar in both groups.
Home Bleaching Effects on the Surface Gloss, Translucency, and Roughness of CAD/CAM Multi-Layered Ceramic and Hybrid Ceramic Materials
Authors: Kandil Mohamed M. , Abdelnabi Ali , Hamdy Tamer M. , Bayoumi Rania E. , Othman Maha S.
Published: December 2024

The surface qualities of CAD/CAM multi-layered ceramic and hybrid ceramic materials are critical for superior aesthetics and may be impaired by the application of home bleaching. The aim of this study was to assess how home bleaching affects the surface gloss, translucency parameter (TP), and surface roughness (Ra, Rq, and Rz) of different CAD/CAM multi-layered ceramic and hybrid ceramic dental materials. The two types of innovative ceramics that were tested are ultra-translucent multi-layered (UTML) zirconia and polymer-infiltrated ceramic blocks. The samples were treated using home bleaching agents. Each specimen was tested under bleached and non-bleached conditions. The surface gloss and TP of the specimens were measured using a spectrophotometer. The surface examination was performed using scanning electron microscope (SEM) images, while the average surface roughness values (Ra, Rq, and Rz) were calculated using three-dimensional SEM images obtained by an imaging analysis system. A total of 120 disc-shaped resin composite specimens was distributed randomly according to each material in two main groups (n = 60): a control group immersed in 20 mL distilled water (non-bleached) (n = 30), and a second group treated with 20 mL of a home bleaching agent (Crest 3D White Multi-Care Whitening Mouthwash) for 60 s, twice daily for seven days (bleached) (n = 30). The surface gloss, TP, and surface roughness (n = 10 per test for each group) of each group (bleached and non-bleached) was tested. An independent sample t-test was used statistically to assess the effect of home bleaching on the surface gloss, translucency, and roughness of each ceramic material and to compare the two materials. The significance level was adjusted at p ≤ 0.05. The results of the bleached UTML specimens showed no significant changes regarding surface gloss, TP, and roughness, whereas the bleached Vita Enamic specimens showed a significant reduction in surface gloss and TP and increased surface roughness. Moreover, the UTML specimens showed a significantly higher initial surface gloss and TP, and a reduced surface roughness, contrary to the Vita Enamic specimens. This study concluded that surface gloss retention, translucency, and surface roughness could be negatively influenced when subjected to home bleaching according to the type and composition of the ceramic materials.
Natural Fiber Reinforcement of Ceramic Slurry Compacts
Authors: Avram Simona Elena , Tudoran Lucian Barbu , Cuc Stanca , Borodi Gheorghe , Birle Bianca Violeta , Petean Ioan
Published: December 2024

Background: ceramic tile wastewater slurry contains a large amount of fine kaolinite particles acting as a matrix for mineral filler particles of quartz and mullite. Reinforcing it with natural fibers increases its compression strength. A novel approach is using Stipa pennata fibers because of their local availability, good mechanical properties, and feathery aspect, making them able to reinforce ceramic slurry compacts. Preparation and investigation methods: Slurry conditioned at 33% humidity and milled at 6000 rpm for 5 min contains 39% quartz, 37% kaolinite, 16% mullite and 8% lepidocrocite (observed via XRD correlated with mineralogical microscopy). Kaolinite particles ensure optimal binding of the mineral filler and the Stipa pennata fibers into a dense composite structure, as observed via SEM. EDS maps reveal a local increase in C content, along with the natural fibers being associated with significant levels of Al and Si, indicating the microstructural compactness of the reinforcement layer. An additional compaction load enhances microstructural cohesion. Results: The sample without reinforcement has a compressive strength of 1.29 MPa. This increases to 2.89 MPa by adding a median reinforcing layer and reaches 3.13 MPa by adding a compaction load of 20 N. A median crossed fiber-reinforcing layer combined with the compaction load of 20 N ensures a compressive strength of 4.78 MPa. Introducing two reinforcing layers oriented perpendicular to one another ensures a compressive strength of 2.48 MPa. Lateral placement of the two reinforcing layers regarding the sample median plan causes a slight decrease in the compressive strength. SEM fractography reveals that the feather-like structure of Stipa pennata fiber acts as an anchor for the median site of the samples, slowing crack initiation under compressive efforts, creating a novel approach compared to natural fiber without lateral flakes. Conclusions: The optimal place for the reinforcement layer is the median site of the sample, and interlaced reinforcement ensures the best compressive resistance. Ceramic slurry reinforced with Stipa pennata is useful as an intermediary layer on the modular walls of ecologic buildings.
Integrated Analytical and Finite Element-Based Modelling, Manufacturing, and Characterisation of Vacuum-Infused Thermoplastic Composite Laminates Cured at Room Temperature
Authors: Beber Vinicius Carrillo , Fernandes Pedro Henrique Evangelista , Nagel Christof , Arnaut Katharina
Published: December 2024

Due to their improved recyclability, thermoplastic composites (TPCs) are increasing their application across industries. The current work deals with the dimensioning, manufacturing, and characterisation of vacuum-infused TPCs cured at RT and made of non-crimp glass fabric and the liquid acrylic-based resin Elium©. Laminates with 10 and 12 layers achieved a fibre weight content of 73% measured by the burn-off process, which corresponds to a fibre volume content of 55%. Three-point bending tests revealed a bending strength of 636.17 ± 25.70 MPa and a bending modulus of 24,600 ± 400 MPa for the 12 layer laminate. Using micro-mechanical models, unidirectional elastic constants are calculated and applied in classical laminate theory (CLT) for optimising composite lay-ups by maximising bending stiffness, whilst yielding a laminate thickness prediction error of −0.18% and a bending modulus prediction error of −1.99%. Additionally, FEA simulations predicted the bending modulus with a −4.47% error and illustrated, with the aid of the Tsai–Hill criterion, the relationship between the onset of layer failure and discrepancies between experimental results and simulations. This investigation demonstrates the effective application of analytical and numerical methods in the dimensioning and performance prediction of TPCs.
Effect of Laser Energy on Anisotropic Material Properties of a Novel Austenitic Stainless Steel with a Fine-Grained Microstructure
Authors: Wang Yurong , Xiao Buwei , Liang Xiaoyu , Zhou Jun , Lin Feng
Published: December 2024

Laser powder bed fusion (LPBF) provides a novel approach with high complexity and freedom for material processing and design, and its special thermal history endows the material with anisotropic properties. By adding micro-alloying elements Nb and Ti into conventional 316L, the anisotropy of the novel austenitic stainless steel fabricated by LPBF, which is related to the laser heat input, was investigated. The refined microstructure of this steel was further strengthened with in situ-generated Nb-, Cr-, and Ti-rich nanoprecipitates at a specific location. The heat input affects the material anisotropy, and a lower heat input leads to stronger anisotropy in this steel. The as-built parts at a low heat input in the horizontal and vertical planes exhibited finer microstructures compared to those fabricated at a high heat input. The epitaxial growth of the grains associated with the thermal gradient resulted in the vertical-section grain size being generally larger than that of the horizontal section. As a result, the low-heat-input parts with a finer grain are also stronger in the horizontal direction, with yield and tensile strengths approaching 0.9 and 1.2 GPa, respectively. Meanwhile, the microstructural changes due to the high heat input imparted a better ductility of parts in different sections (a 3.15% and 4.4% increase in the horizontal and vertical directions, respectively). Its mechanical properties depend mainly on the direction of stress coupled with intergranular friction during deformation in both coarse and fine grains.
Research on the Comparison of the Flow Evolution Mechanisms of a Water-Jet Pump Between Valley and Peak Conditions
Authors: Liu Min , Long Yun , Zheng Yingying , Zhong Jinqing , Yin Hong
Published: December 2024

The pump hump significantly influences the vibration and operational stability of pumps. During the development of the mixed-flow waterjet pump, our team found that the pump performance curve had a hump phenomenon and the platform had vibration, so it was suspected that there was a strong secondary flow in the hump region. The calculation model is the SST k-ω turbulence model. The impeller and diffuser use structured grids. By using high-speed photography technology, we map the cavitation flow structures, thereby demonstrating the evolution of cavitation processes. The hump curve was obtained by an experimental test. By comparing the test data and numerical simulation, the consistency of the method and the hump curve is verified. A comparative analysis is performed to investigate the variations in the distribution of internal vortex structures and the evolution of rotating stalls in the impeller. In the valley condition, the main frequency of pressure pulsation in the inlet section of the impeller is 0.75 times the shaft frequency, the main frequency in the middle and outlet sections of the impeller is 1 times the shaft frequency, and the main frequency in the diffuser basin is 1.5 times the shaft frequency, the main frequency in the peak condition at the points of Span = 0.1 and Span = 0.5 of the impeller is the diffuser frequency, the main frequency at the point of Span = 0.9 is the impeller blade frequency, and the main frequency in the diffuser basin is either the shaft frequency or the diffuser blade frequency. This research reveals the characteristics of vortex flow in the pump under hump conditions. It reveals that the evolution mechanism of the hump offers a guide value for the subsequent hydrodynamic design of the hump.
Efficacy and Safety of Intra-Class Switching from Ixekizumab to Secukinumab in Patients with Plaque Psoriasis: A Multicenter Retrospective Study
Authors: Bernardini Nicoletta , Dattola Annunziata , Rossi Raimondo , Pagnanelli Gianluca , Amerio Paolo , Atzori Laura , Mugheddu Cristina , Lora Viviana , Giordano Domenico , Guacci Lucia Finistauri , Persechino Severino , Richetta Antonio Giovanni , Skroza Nevena , Potenza Concetta
Published: December 2024

Background: the present multicenter retrospective study aimed to evaluate the efficacy and safety of intra-class switching between interleukin-17A (IL-17A) inhibitors, specifically from ixekizumab to secukinumab, in patients with plaque psoriasis. Methods: this study included 11 patients (6 male, 5 female) who had previously received ixekizumab and then were switched to secukinumab. Patients’ PASI, DLQI, and pain VAS (in those with psoriatic arthritis) were evaluated at weeks 16, 24, 54, and 98. Results: PASI-90 was reached in 10 (91%) cases at week 24. One patient experienced secondary failure to secukinumab at week 98. No adverse events were reported. Conclusions: overall, the majority of patients experienced a favorable response to secukinumab, suggesting that it may be an effective treatment option for patients with an inadequate response or loss of efficacy to ixekizumab.
Adult Outcomes of Childhood Wheezing Phenotypes Are Associated with Early-Life Factors
Authors: Carra Sophie , Zhang Hongmei , Tanno Luciana Kase , Arshad Syed Hasan , Kurukulaaratchy Ramesh J.
Published: December 2024

Introduction: While the phenotypic diversity of childhood wheezing is well described, the subsequent life course of such phenotypes and their adult outcomes remain poorly understood. We hypothesized that different childhood wheezing phenotypes have varying longitudinal outcomes at age 26. We sought to identify factors associated with wheezing persistence, clinical remission, and new onset in adulthood. Methods: Participants were seen at birth and at 1, 2, 4, 10, 18, and 26 years in the Isle of Wight Birth Cohort (n = 1456). Information was collected prospectively on wheeze prevalence and phenotypic characteristics at each assessment. Wheeze phenotypes at 10 years were defined as participants wheezing (CW10) or not wheezing at 10 (CNW10). Multivariable regression analyses were undertaken to identify factors associated with wheezing persistence/remission in CW10 and wheeze development in CNW10 at age 26 years. Results: Childhood wheezing phenotypes showed different subsequent outcomes and associated risk factors. Adult wheeze developed in 17.8% of CNW10. Factors independently associated with adult wheeze development in CNW10 included eczema at age 4 years, family history of rhinitis, and parental smoking at birth. Conversely, 56.1% of CW10 had remission of wheeze by 26 years. Factors predicting adult wheezing remission in CW10 included absence of both atopy at age 4 years and family history of rhinitis. Conclusion: Early-life factors influence adult outcomes for childhood wheezing phenotypes, both with respect to later development of adult wheezing in asymptomatic participants and of wheeze remission in childhood wheezers. This suggests potential areas that could be targeted by early-life interventions to alleviate adult disease burden.
Metagenome Analysis Identified Novel Microbial Diversity of Sandy Soils Surrounded by Natural Lakes and Artificial Water Points in King Salman Bin Abdulaziz Royal Natural Reserve, Saudi Arabia
Authors: Al-Awthan Yahya S. , Mir Rashid , Alatawi Fuad A. , Alatawi Abdulaziz S. , Almutairi Fahad M. , Khafaga Tamer , Shohdi Wael M. , Fakhry Amal M. , Alharbi Basmah M.
Published: December 2024

Background: Soil microbes play a vital role in the ecosystem as they are able to carry out a number of vital tasks. Additionally, metagenomic studies offer valuable insights into the composition and functional potential of soil microbial communities. Furthermore, analyzing the obtained data can improve agricultural restoration practices and aid in developing more effective environmental management strategies. Methodology: In November 2023, sandy soil samples were collected from ten sites of different geographical areas surrounding natural lakes and artificial water points in the Tubaiq conservation area of King Salman Bin Abdulaziz Royal Natural Reserve (KSRNR), Saudi Arabia. In addition, genomic DNA was extracted from the collected soil samples, and 16S rRNA sequencing was conducted using high-throughput Illumina technology. Several computational analysis tools were used for gene prediction and taxonomic classification of the microbial groups. Results: In this study, sandy soil samples from the surroundings of natural and artificial water resources of two distinct natures were used. Based on 16S rRNA sequencing, a total of 24,563 OTUs were detected. The metagenomic information was then categorized into 446 orders, 1036 families, 4102 genera, 213 classes, and 181 phyla. Moreover, the phylum Pseudomonadota was the most dominant microbial community across all samples, representing an average relative abundance of 34%. In addition, Actinomycetes was the most abundant class (26%). The analysis of clustered proteins assigned to COG categories provides a detailed understanding of the functional capabilities and adaptation of microbial communities in soil samples. Amino acid metabolism and transport were the most abundant categories in the soil environment. Conclusions: Metagenome analysis of sandy soils surrounding natural lakes and artificial water points in the Tubaiq conservation area of KSRNR (Saudi Arabia) has unveils rich microbial activity, highlighting the complex interactions and ecological roles of microbial communities in these environments.
Incidence of Total Knee Arthroplasty in Older Females with Knee Osteoarthritis and Osteoporosis Treated with Denosumab Compared with Those Treated Using Bisphosphonates: A Population-Based Cohort Study
Authors: Yu Tzai-Chiu , Wu Wen-Tien , Lee Ru-Ping , Chen Ing-Ho , Wang Jen-Hung , Wen Shu-Hui , Yeh Kuang-Ting
Published: December 2024

This study aimed to evaluate the incidence of total knee arthroplasty (TKA), a marker of severe knee osteoarthritis (OA), among older females with concurrent knee OA and osteoporosis (OP) who were treated with denosumab or bisphosphonates. By analyzing a large population-based cohort, we sought to clarify how these treatments influence the progression of knee OA to the point of requiring surgical intervention. We used data from the Taiwan National Health Insurance Research Database, including data from females aged ≥ 50 years diagnosed with knee OA and OP who initiated treatment between 2012 and 2019. Propensity score matching (1:1) resulted in the selection of 13,774 patients (6897 per group). The TKA incidence was analyzed using Cox proportional hazards models. Patients treated with denosumab had a lower TKA incidence than those treated with bisphosphonates (6.9 vs. 8.5 per 1000 person-years). The adjusted hazard ratio (aHR) for TKA in the denosumab group was 0.77 (95% CI: 0.62–0.97; p = 0.024), with the most pronounced effect observed in patients aged ≥ 80 years (aHR = 0.39, 95% CI: 0.20–0.77; p = 0.007). These findings suggest that denosumab reduces TKA risk more effectively than bisphosphonates and may serve as a superior treatment option for mitigating severe knee OA progression, especially in older adults.
Identification of miRNAs Involved in Olfactory Regulation in Antennae of Beet Webworm, Loxostege sticticalis (Lepidoptera: Pyralidae)
Authors: Zhang Yu , Li Yanyan , Han Haibin , Wang Xiaoling , Gao Shujing , Zhao Qing , Bieerdebieke Halima , Xu Linbo , Zang Qicong , Wang Hui , Bai Penghua , Lin Kejian
Published: December 2024

The beet webworm, Loxostege sticticalis, is a typical migratory pest. Although miRNAs participate in many physiological functions, little is known about the functions of miRNAs in olfactory regulation. In this study, 1120 (869 known and 251 novel) miRNAs were identified in the antennae of L. sticticalis by using high-throughput sequencing technology. Among the known miRNAs, 189 from 49 families were insect-specific, indicating that these miRNAs might play unique roles in insects. Furthermore, based on the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses, we found that 3647 and 1393 miRNAs were associated with localization and the regulation of localization, respectively, and 80 miRNAs were enriched in the neuroactive ligand–receptor interaction pathway. These miRNAs might be involved in the olfactory system of L. sticticalis. Notably, qRT-PCR showed that most of the tested miRNAs presented similar expression patterns compared with the RNA-seq data and that miR-87-3, novel-miR-78, and novel-miR-142 were significantly differentially expressed in the antennae of males and females. In addition, 21 miRNAs were predicted to target 23 olfactory genes, including 10 odorant-binding proteins (OBPs), 3 chemosensory proteins (CSPs), 4 odorant receptors (ORs), 1 ionotropic receptor (IR), and 5 gustatory receptors (GRs). The olfactory-related miRNAs exhibited low-abundance transcripts, except undef-miR-55 and undef-miR-523, and gender-biased expression was not observed for olfactory-related miRNAs. Our findings provide an overview of the potential miRNAs involved in olfactory regulation, which may provide important information on the function of miRNAs in the insect olfactory system.
High-Order Control Lyapunov–Barrier Functions for Real-Time Optimal Control of Constrained Non-Affine Systems
Authors: Chriat Alaa Eddine , Sun Chuangchuang
Published: December 2024

This paper presents a synthesis of higher-order control Lyapunov functions (HOCLFs) and higher-order control barrier functions (HOCBFs) capable of controlling nonlinear dynamic systems while maintaining safety. Building on previous Lyapunov and barrier formulations, we first investigate the feasibility of the Lyapunov and barrier function approach in controlling a non-affine dynamic system under certain convexity conditions. Then we propose an HOCLF form that ensures convergence of non-convex dynamics with convex control inputs to target states. We combine the HOCLF with the HOCBF to ensure forward invariance of admissible sets and guarantee safety. This online non-convex optimal control problem is then formulated as a convex Quadratic Program (QP) that can be efficiently solved on board for real-time applications. Lastly, we determine the HOCLBF coefficients using a heuristic approach where the parameters are tuned and automatically decided to ensure the feasibility of the QPs, an inherent major limitation of high-order CBFs. The efficacy of the suggested algorithm is demonstrated on the real-time six-degree-of-freedom powered descent optimal control problem, where simulation results were run efficiently on a standard laptop.
Lithium Coupled with C6-Carboxyl Improves the Efficacy of Oligoguluronate in DSS-Induced Ulcerative Colitis in C57BL/6J Mice
Authors: Li Jiayi , Shao Meng , Liu Hao , Guo Peng , Liu Fei , Ma Mingfeng , Li Quancai
Published: December 2024

Oligoguluronate lithium (OGLi) was prepared for the purpose of enhancing the anti-ulcerative colitis (UC) activities of OG, in which lithium (Li+) is coupled with the C6-carboxyl of G residue. The therapeutic effects of OGLi on dextran sulfate (DSS)-induced UC mice were investigated, and oligoguluronate sodium (OGNa) and lithium carbonate (LC) were used as contrasts. The effects of OGLi, OGNa and LC on the treatment of UC mice were studied by monitoring body weight change and evaluating colon length, the disease activity index (DAI), histopathological examination and gut microbiota regulation. The results showed that compared with OGNa and LC, OGLi significantly reduced the clinical symptoms and histopathological changes associated with UC in the acute model. It was worth noting that OGLi significantly changed the gut microbiota characteristics of the DSS-treated mice and corrected the typical dysbacteriosis of DSS-induced UC. This intervention resulted in increasing the abundance of norank_f_Muribaculaceae and Ileibacterium spp. while reducing the levels of Escherichia-Shigella spp. and Romboutsia spp. The OGLi could significantly increase the diversity of intestinal microorganisms in the short term. All of these discoveries demonstrate that lithium collaboratively enhances the anti-UC efficacy of OG, which will help to create OG-based drugs for the treatment of UC.
Metabolic Dysfunction-Associated Steatotic Liver Disease in Type 2 Diabetes Patients—The Relationship with Platelets Indicators
Authors: Onișor Danusia , Roiban Andrada Larisa , Cernea Simona
Published: December 2024

Background and Objectives: Metabolic dysfunction-associated steatotic liver disease (MASLD) is an important chronic liver disease with major health risks, especially in the presence of T2DM, but the pathophysiology of this condition is not fully understood. This study aimed to investigate the platelet hematometric indices in patients with T2DM and MASLD. Materials and Methods: Demographic and medical (including anthropometric) data were collected from 271 participants, from whom blood samples were also drawn in fasting conditions for complete blood count, liver and metabolic panel, ferritin, haptoglobin, creatinine, and fibrosis markers. The correlations of main platelet parameters with clinical and laboratory data were investigated by bivariate and multiple regression analyses. Results: The median platelets number was 235·103/μL, and thus, the study population was divided into two subgroups: with higher and lower numbers (group 1 (mean): 286.38 ± 43.29·103/μL and group 2 (mean): 188.12 ± 39.77·103/μL). Despite similar BMIs, group 2 had higher fatty liver index (FLI) (84.44 ± 18.04 vs. 79.85 ± 17.98; p = 0.0088) and insulin resistance (HOMA-IR: 3.16 ± 1.50 vs. 2.63 ± 1.31; 0.0008), higher direct bilirubin, transaminases, uric acid, and ferritin concentrations. Higher percentages of males and subjects with HOMA-IR values >2.5 were accounted for in this group. In the multiple regression analyses, the platelet count and plateletcrit (PTC) correlated independently with sex, leucocyte count, HOMA-IR, and bilirubin concentrations (p < 0.0001). The platelet distribution width (PDW) was positively correlated with insulin resistance in two separate analyses (β = 0.060; p = 0.0004, and β = 0.052; p = 0.0025), and with GGT, while the mean platelet volume presented a weak but significant positive association with FLI. Patients with higher HOMA-IR had higher PDW and a lower platelet count and PTC. Conclusions: Male patients with T2DM and MASLD had lower platelet count and PTC and larger PDW. Higher insulin resistance was associated with lower platelet count and PTC and higher PDW.
Hepatitis C Virus Infection in Hemodialysis Patients in the Era of Direct-Acting Antiviral Treatment: Observational Study and Narrative Review
Authors: Ratiu Ioana Adela , Mihaescu Adelina , Olariu Nicu , Ratiu Cristian Adrian , Cristian Bako Gabriel , Ratiu Anamaria , Indries Mirela , Fratila Simona , Dejeu Danut , Teusdea Alin , Ganea Mariana , Moisa Corina , Marc Luciana
Published: December 2024

Background and Objectives: Hepatitis C virus (HCV) infection is a major global public health concern, particularly in hemodialysis (HD) patients. This study aims to evaluate the demographic, clinical, and laboratory characteristics of HCV-positive patients undergoing HD and assess the long-term impact of direct-acting antivirals (DAAs) on patient outcomes. Moreover, a narrative review aims to summarize the current knowledge regarding HCV treatment in HD patients. The search in the PubMed, Google Scholar, and Scopus databases identified 48 studies relevant to our topic, 18 regarding clinical history and 29 related to HCV treatment. Methods: A retrospective analysis was performed on 165 HD patients from Bihor County HD centers, Romania, between 2014 and 2024. The cohort was divided into two groups: 54 patients who tested positive for HCV and 111 controls who were HCV-negative. Data collected from GPs included demographic information, comorbidities, laboratory parameters, and psychological assessments. Outcomes were evaluated at over 5 years after DAA treatment. A literature review was conducted using PubMed and Google Scholar to identify relevant studies on HCV in HD patients from 1989 to 2024. Results: Laboratory results showed similar parameters across groups, except for lower serum cholesterol levels in the HCV-positive DAA-treated group vs. HCV-positive non-treated ones (155.607 mg% vs. 170.174 mg%, p = 0.040) and increased ALT levels when comparing the same groups (29.107 vs. 22.261, p = 0.027), whereas comorbidities did not differ significantly. The incidence of malignancies was significantly higher among HCV-positive compared to HCV-negative patients (20.3% vs. 8.1%, p = 0.023), mainly among those treated with DAAs, highlighted by the multivariate analysis. Cardiovascular disease remains the leading cause of mortality regardless of HCV status or the use of antiviral therapy. Psychological assessments revealed more severe depression in HCV-positive patients compared to their HCV-negative counterparts. Conclusions: HCV infection in the hemodialysis population typically follows a subclinical course. At over five years after DAA therapy, the results indicate a stabilization of the liver function and the absence of major complications. However, the incidence of malignancies remains high in HCV-positive patients.
Epidemiology of Rounding Error
Authors: Efird Jimmy T.
Published: December 2024

This work represents a significant contribution to understanding the importance of appropriately rounding numbers with minimal error. That is, to reduce inexact rounding and data truncation error and simultaneously eliminate unintentional misleading findings in epidemiological studies. The rounding of numbers represents a compromise solution that attempts to find a balance between the loss of information from reporting too few significant digits versus retaining more digits than necessary. Substituting a rounded number for its original value may be acceptable and practical in many applied situations if an adequate degree of accuracy is retained. On the other hand, numeric error may result from improper rounding or data truncation which, in effect, compromises the credibility of study findings and may lead to a false sense of discovery. Performing complex computations on such values, especially when sequential or composite operations are involved, can lead to error propagation and inaccurate results. Having an overall awareness of the nature and impact of rounding error, including preventive actions, can contribute greatly to the integrity of research, yielding more reliable and accurate conclusions. Heuristic examples are provided to illustrate the consequences of rounding and data truncation error in epidemiology studies, specifically those pertaining to relative effect estimation.
Craniocervical Posture and Malocclusion: A Comprehensive Literature Review of Interdisciplinary Insights and Implications
Authors: Kui Andreea , Bereanu Alexandru , Condor Ana-Maria , Pop Dalia , Buduru Smaranda , Labunet Anca , Șoicu Sebastian , Buduru Rareș , Chisnoiu Andrea
Published: December 2024

Background and Objectives: The impact of craniocervical posture on malocclusion has long intrigued researchers in dentistry, orthodontics, and physical therapy. This research aims to elucidate the relationship between craniocervical posture and both dental and skeletal malocclusions and to explore the potential for integrated multidisciplinary therapeutic approaches. Materials and Methods: We analyzed peer-reviewed articles published between 2013 and 2023 from PubMed/Medline, Web of Science, EMBASE, and Scopus. The search strategy included terms related to craniocervical posture and malocclusion, focusing on studies that evaluated the relationship between these conditions before and after various orthodontic or surgical treatments. Results: A total of 20 studies met the inclusion criteria, providing nuanced insights into the interplay between malocclusion types and craniocervical alignment. Findings suggest that altered craniocervical posture is more prevalent in individuals with skeletal malocclusions. In particular, orthodontic treatment and orthognathic surgery appear to influence craniocervical posture, suggesting a bidirectional relationship between craniofacial structure and neck alignment. Conclusions: Our literature review confirms a significant association between craniocervical posture and malocclusion, emphasizing the need for an integrative approach to the diagnosis and treatment of craniofacial anomalies. Future research should aim to quantify these relationships further through longitudinal studies, thereby increasing the understanding necessary to develop comprehensive treatment protocols.