bluuebunny/crossref_metadata_2025 huggingface dataset:
Homogeneous Ensemble Feature Selection for Mass Spectrometry Data Prediction in Cancer Studies
Authors: Liang Yulan , Gharipour Amin , Kelemen Erik , Kelemen Arpad
Published: July 2024

The identification of important proteins is critical for the medical diagnosis and prognosis of common diseases. Diverse sets of computational tools have been developed for omics data reduction and protein selection. However, standard statistical models with single-feature selection involve the multi-testing burden of low power with limited available samples. Furthermore, high correlations among proteins with high redundancy and moderate effects often lead to unstable selections and cause reproducibility issues. Ensemble feature selection in machine learning (ML) may identify a stable set of disease biomarkers that could improve the prediction performance of subsequent classification models and thereby simplify their interpretability. In this study, we developed a three-stage homogeneous ensemble feature selection (HEFS) approach for both identifying proteins and improving prediction accuracy. This approach was implemented and applied to ovarian cancer proteogenomics datasets comprising (1) binary putative homologous recombination deficiency (HRD)- positive or -negative samples; (2) multiple mRNA classes (differentiated, proliferative, immunoreactive, mesenchymal, and unknown samples). We conducted and compared various ML methods with HEFS including random forest (RF), support vector machine (SVM), and neural network (NN) for predicting both binary and multiple-class outcomes. The results indicated that the prediction accuracies varied for both binary and multiple-class classifications using various ML approaches with the proposed HEFS method. RF and NN provided better prediction accuracies than simple Naive Bayes or logistic models. For binary outcomes, with a sample size of 122 and nine selected prediction proteins using our proposed three-stage HEFS approach, the best ensemble ML (Treebag) achieved 83% accuracy, 85% sensitivity, and 81% specificity. For multiple (five)-class outcomes, the proposed HEFS-selected proteins combined with Principal Component Analysis (PCA) in NN resulted in prediction accuracies for multiple-class classifications ranging from 75% to 96% for each of the five classes. Despite the different prediction accuracies of the various models, HEFS identified consistent sets of proteins linked to the binary and multiple-class outcomes.
Testing a Class of Piece-Wise CHARN Models with Application to Change-Point Study
Authors: Salman Youssef , Ngatchou-Wandji Joseph , Khraibani Zaher
Published: July 2024

We study a likelihood ratio test for testing the conditional mean of a class of piece-wise stationary CHARN models. We establish the locally asymptotically normal (LAN) structure of the family of likelihoods under study. We prove that the test is asymptotically optimal, and we give an explicit form of its asymptotic local power. We describe an algorithm for detecting change points and estimating their locations. The estimates are obtained as time indices, maximizing the estimate of the local power. The simulation study we conduct shows the good performance of our method on the examples considered. This method is also applied to a set of financial data.
Enhancing Decision-Making Processes in the Complex Landscape of the Taiwanese Electronics Manufacturing Industry through a Fuzzy MCDM Approach
Authors: Chen Wen-Chin , Ngo An-Xuan , Chang Hui-Pin
Published: July 2024

This research proposes a hybrid multi-criteria decision-making (MCDM) framework for workforce recruitment in Taiwan’s electronics manufacturing companies, an area with limited research. First, a comprehensive review of existing literature and interviews with industry experts were conducted to compile a list of decision-making criteria and sub-criteria relevant to workforce selection in Taiwan’s electronics industry. The Fuzzy Delphi Method (FDM) was then applied to identify and retain the most critical criteria while eliminating the less important ones. Next, Interpretive Structural Modelling (ISM) was used to calculate the interdependencies among the identified factors. Finally, based on these relationships, the Fuzzy Analytic Network Process (FANP) was employed to calculate the relative importance weights of the criteria and sub-criteria. These weights were then used to rank the criteria, identifying the most important ones and aiding in decision-making. The findings indicate that the proposed method provides a structured and assessable model for making informed decisions in workforce recruitment, particularly in the challenging environment of Taiwan’s electronics manufacturing industry, which faces a shortage of skilled labor. The research presents three primary contributions: the development of a systematic criteria selection technique using FDM, the establishment of consistent criteria relations for decision-makers using ISM, and the proposal of an application model employing the FANP method to identify and rank appropriate criteria for hiring new employees. The study highlights work attitude, adaptability to the environment, and work ability as major criteria. It also emphasizes the importance of discipline compliance, a positive attitude, and adherence to health and safety protocols as the top sub-criteria for workforce selection.
Robust Stability Analysis of Switched Neural Networks with Application in Psychological Counseling Evaluation System
Authors: Li Yajuan , Xue Huanbin
Published: July 2024

In this work, the effectiveness and stability of psychological counseling are evaluated using the switched complex-valued neural networks (SCVNN) model, which includes parameter disturbances, impulsive perturbations, variable and continuously distributed delays in the system state, and impulsive delay. How to analyze and judge the stability of the network simply and effectively is the primary prerequisite for its successful application. Therefore, we explore the dynamic behavior of SCVNN with both variable and distributed delays along with impulsive effect. Initially, the proposed conditions for the existence and uniqueness of equilibrium in SCVNN are presented. Subsequently, employing the inequality technique and impulsive average dwell time approach, sufficient conditions for the robust exponential stability of SCVNN under both arbitrary and restricted switching are obtained. Lastly, the psychological counseling evaluation system (PCES) is established, and a simulation example is used to verify the correctness and effectiveness of the presented findings.
Assessing the Mediating Effect of Team-Member Exchange on the Relationship between Transformational Leadership and Performance of People with Disabilities: A Study of Hong Kong Employers of People with Disabilities
Authors: Wong Anthony Tak Kin
Published: July 2024

Purpose—This study aims to examine the links between transformational leadership, team-member exchange and job performance of people with disabilities in Hong Kong. Design/methodology/approach—Data were collected from a sample of 108 direct supervisors of people with disabilities via an online survey with validated scales. The structural equation modelling approach via SmartPLS (Version 3.0) was used to investigate the linear relations and the mediated effect amongst the variables. Findings—Transformational leadership was positively related to team-member exchange (p = 0.013), but its link with job performance was insignificant (p ≥ 0.05). Team-member exchange was positively related to job performance (p = 0.000). Hence, team-member exchange fully mediated the relationship between leaders’ transformational leadership and the job performance of people with disabilities. Originality/value—Transformational leadership is favorable for the job performance of people with disabilities, but its influence is indirect. High level of team-member exchange quality is necessary, as team-member exchange fully mediates the relationship between transformational leadership and the job performance of people with disabilities. Managers should demonstrate transformational leadership to all employees and not only pay attention to people with disabilities. This insight helps drive workplace disability inclusion.
Pathways toward Climate-Neutral Red Meat Production
Authors: Ridoutt Bradley
Published: July 2024

Ruminant livestock industries can support the climate stabilization ambitions of the Paris Agreement through interventions that reduce GHG emissions (predominantly biogenic methane) and sequester carbon in landscapes. This study explored pathways for the Australian red meat industry (grazing, feedlot finishing, and domestic processing) to become climate neutral, whereby the radiative forcing (RF) footprint is plateaued and there is no additional forcing contribution. Emissions timeseries (CO2, N2O, CH4) were compiled for 1990 to 2020 and projected to 2030 under a business-as-usual scenario (including an 18% increase in sheep and 13% increase in beef cattle) and with a range of production system and vegetation management interventions. The RF footprint peaked in 2018 at 7.13 mW/m2 and decreased to 7.07 mW/m2 in 2020. With the future expansion of the herd/flock and under business-as-usual conditions, the RF footprint is projected to increase by 2.8% by 2030. However, with a combination of interventions, production has the potential to increase with a decreasing RF footprint, a condition that can be described as climate neutral. The Australian red meat industry has made an historical contribution to global RF increase. However, with ongoing RF management, it is possible to increase food production within climate-neutral limits.
Amazonian Bacteria from River Sediments as a Biocontrol Solution against Ralstonia solanacearum
Authors: Fonseca Jennifer Salgado da , Sousa Thiago Fernandes , Almeida Suene Vanessa Reis de , Silva Carina Nascimento , Castro Gleucinei dos Santos , Yamagishi Michel Eduardo Beleza , Koolen Hector Henrique Ferreira , Hanada Rogério Eiji , Silva Gilvan Ferreira da
Published: July 2024

Bacterial wilt, caused by Ralstonia solanacearum, is one of the main challenges for sustainable tomato production in the Amazon region. This study evaluated the potential of bacteria isolated from sediments of the Solimões and Negro rivers for the biocontrol of this disease. From 36 bacteria selected through in vitro antibiosis, three promising isolates were identified: Priestia aryabhattai RN 11, Streptomyces sp. RN 24, and Kitasatospora sp. SOL 195, which inhibited the growth of the phytopathogen by 100%, 87.62%, and 100%, respectively. These isolates also demonstrated the ability to produce extracellular enzymes and plant growth-promoting compounds, such as indole-3-acetic acid (IAA), siderophore, and ammonia. In plant assays, during both dry and rainy seasons, P. aryabhattai RN 11 reduced disease incidence by 40% and 90%, respectively, while promoting the growth of infected plants. Streptomyces sp. RN 24 and Kitasatospora sp. SOL 195 exhibited high survival rates (85–90%) and pathogen suppression in the soil (>90%), demonstrating their potential as biocontrol agents. This study highlights the potential of Amazonian bacteria as biocontrol agents against bacterial wilt, contributing to the development of sustainable management strategies for this important disease.
Development of Polymerase Chain Reaction–High-Resolution Melt Assay for Waterborne Pathogens Legionella pneumophila, Vibrio parahaemolyticus, and Camplobacter jejuni
Authors: Carr Shannon M. , Elkins Kelly M.
Published: July 2024

Legionella pneumophila is the waterborne pathogen primarily responsible for causing both Pontiac Fever and Legionnaire’s Disease in humans. L. pneumophila is transmitted via aerosolized water droplets. The purpose of this study was to design and test primers to allow for rapid polymerase chain reaction (PCR) melt detection and identification of this infectious agent in cases of clinical or emergency response detection. New PCR primers were designed for this species of bacteria; the primer set was purchased from IDT and the target bacterial DNA was purchased from ATCC. The L. pneumophila primers targeted the macrophage infectivity potentiator gene (mip), which inhibits macrophage phagocytosis. The primers were tested for specificity, repeatability, and sensitivity using PCR–high-resolution melt (HRM) assays. The primer set was found to be specific to the designated bacteria and did not amplify the other twenty-one species from the panel. The L. pneumophila assay was able to be multiplexed. The duplex assay consists of primers for L. pneumophila and Vibrio parahaemolyticus, which are both waterborne pathogens. The triplex assay consists of primers for L. pneumophila, V. parahaemolyticus, and Campylobacter jejuni. The unique melting temperature for the L. pneumophila primer assay is 82.84 ± 0.19 °C, the C. jejuni assay is 78.10 ± 0.58 °C, and the V. parahaemolyticus assay is 86.74 ± 0.65 °C.
The Use of External Fields (Magnetic, Electric, and Strain) in Molecular Beam Epitaxy—The Method and Application Examples
Authors: Dziwoki Adam , Blyzniuk Bohdana , Freindl Kinga , Madej Ewa , Młyńczak Ewa , Wilgocka-Ślęzak Dorota , Korecki Józef , Spiridis Nika
Published: July 2024

Molecular beam epitaxy (MBE) is a powerful tool in modern technologies, including electronic, optoelectronic, spintronic, and sensoric applications. The primary factor determining epitaxial heterostructure properties is the growth mode and the resulting atomic structure and microstructure. In this paper, we present a novel method for growing epitaxial layers and nanostructures with specific and optimized structural and magnetic properties by assisting the MBE process using electromagnetic and mechanical external stimuli: an electric field (EF), a magnetic field (MF), and a strain field (SF). The transmission of the external fields to the sample is realized using a system of specialized sample holders, advanced transfers, and dedicated manipulators. Examples of applications include the influence of MFs on the growth and anisotropy of epitaxial magnetite and iron films, the use of EFs for in situ resistivity measurements, the realization of in situ magneto-optic measurements, and the application of SFs to the structural modification of metal films on mica.
Application of Photoactive Compounds in Cancer Theranostics: Review on Recent Trends from Photoactive Chemistry to Artificial Intelligence
Authors: Szymaszek Patryk , Tyszka-Czochara Małgorzata , Ortyl Joanna
Published: July 2024

According to the World Health Organization (WHO) and the International Agency for Research on Cancer (IARC), the number of cancer cases and deaths worldwide is predicted to nearly double by 2030, reaching 21.7 million cases and 13 million fatalities. The increase in cancer mortality is due to limitations in the diagnosis and treatment options that are currently available. The close relationship between diagnostics and medicine has made it possible for cancer patients to receive precise diagnoses and individualized care. This article discusses newly developed compounds with potential for photodynamic therapy and diagnostic applications, as well as those already in use. In addition, it discusses the use of artificial intelligence in the analysis of diagnostic images obtained using, among other things, theranostic agents.
Liquid–Liquid and Liquid–Solid Interfacial Nanoarchitectonics
Authors: Ariga Katsuhiko
Published: July 2024

Nanoscale science is becoming increasingly important and prominent, and further development will necessitate integration with other material chemistries. In other words, it involves the construction of a methodology to build up materials based on nanoscale knowledge. This is also the beginning of the concept of post-nanotechnology. This role belongs to nanoarchitectonics, which has been rapidly developing in recent years. However, the scope of application of nanoarchitectonics is wide, and it is somewhat difficult to compile everything. Therefore, this review article will introduce the concepts of liquid and interface, which are the keywords for the organization of functional material systems in biological systems. The target interfaces are liquid–liquid interface, liquid–solid interface, and so on. Recent examples are summarized under the categories of molecular assembly, metal-organic framework and covalent organic framework, and living cell. In addition, the latest research on the liquid interfacial nanoarchitectonics of organic semiconductor film is also discussed. The final conclusive section summarizes these features and discusses the necessary components for the development of liquid interfacial nanoarchitectonics.
A Heterogeneous Viscosity Flow Model for Liquid Transport through Nanopores Considering Pore Size and Wettability
Authors: Chang Yilin , Zhang Yapu , Niu Zhongkun , Chen Xinliang , Du Meng , Yang Zhengming
Published: July 2024

The confinement effect in micro- and nanopores gives rise to distinct flow characteristics in fluids. Clarifying the fluid migration pattern in confined space is crucial for understanding and explaining the abnormal flow phenomena in unconventional reservoirs. In this study, flow characteristics of water and oil in alumina nanochannels were investigated with diameters ranging from 21 nm to 120 nm, and a heterogeneous viscosity flow model considering boundary fluid was proposed. Compared with the prediction of the HP equation, both types of fluids exhibit significant flow suppression in nanochannels. As the channel size decreases, the deviation degree increases. The fluid viscosity of the boundary region displays an upward trend as the channel size decreases and the influence of the interaction between the liquid and solid walls intensifies. The thickness of the boundary region gradually decreases with increasing pressure and eventually reaches a stable value, which is primarily determined by the strength of the interaction between the liquid and solid surfaces. Both the pore size and wettability are essential factors that affect the fluid flow. When the space scale is extremely small, the impact of wettability becomes more pronounced. Finally, the application of the heterogeneous flow model for permeability evaluation has yielded favorable fitting results. The model is of great significance for studying the fluid flow behavior in unconventional reservoirs.
Heterogeneous Iron-Based Catalysts for Organic Transformation Reactions: A Brief Overview
Authors: Baruah Manash J. , Dutta Rupjyoti , Zaki Magdi E. A. , Bania Kusum K.
Published: July 2024

Iron (Fe) is considered to be one of the most significant elements due to its wide applications. Recent years have witnessed a burgeoning interest in Fe catalysis as a sustainable and cost-effective alternative to noble metal catalysis in organic synthesis. The abundance and low toxicity of Fe, coupled with its competitive reactivity and selectivity, underscore its appeal for sustainable synthesis. A lot of catalytic reactions have been performed using heterogeneous catalysts of Fe oxide hybridized with support systems like aluminosilicates, clays, carbonized materials, metal oxides or polymeric matrices. This review provides a comprehensive overview of the latest advancements in Fe-catalyzed organic transformation reactions. Highlighted areas include cross-coupling reactions, C–H activation, asymmetric catalysis, and cascade processes, showcasing the versatility of Fe across a spectrum of synthetic methodologies. Emphasis is placed on mechanistic insights, elucidating the underlying principles governing iron-catalyzed reactions. Challenges and opportunities in the field are discussed, providing a roadmap for future research endeavors. Overall, this review illuminates the transformative potential of Fe catalysis in driving innovation and sustainability in organic chemistry, with implications for drug discovery, materials science, and beyond.
Expression of Transposable Elements throughout the Fasciola hepatica Trematode Life Cycle
Authors: Skalon Elizaveta K. , Panyushev Nick V. , Podgornaya Olga I. , Smolyaninova Anastasia R. , Solovyeva Anna I.
Published: July 2024

Background: Transposable elements (TEs) are major components of eukaryotic genomes. The extensive body of evidence suggests that although they were once considered “genomic parasites”, transposons and their transcripts perform specific functions, such as regulation of early embryo development. Understanding the role of TEs in such parasites as trematodes is becoming critically important. Fasciola hepatica, a parasite affecting humans and livestock, undergoes a complex life cycle in diverse environments and hosts, and knowledge about its life cycle regulation is scarce so far. Methods: We summarized the data regarding the repetitive elements in F. hepatica and conducted bulk RNA-seq analysis across its life cycle stages. TE expression profiles were analyzed, focusing on differential expression and potential homology with previously described long non-coding RNAs (lncRNAs). Results: Differential expression analysis revealed stage-specific TE transcription patterns, notably peaking during egg and metacercariae stages. Some TEs showed homology with known lncRNAs and contained putative transcription factor binding sites. Interestingly, TE transcription levels were highest in eggs and metacercariae compared to adults, suggesting regulatory roles in trematode life cycle transitions. Conclusions: These findings suggest that TEs may play roles in regulating trematode life cycle transitions. Moreover, TE homology with lncRNAs underscores their significance in gene regulation.
Feeding Mechanisms of Pathogenic Protozoa with a Focus on Endocytosis and the Digestive Vacuole
Authors: Wiser Mark F.
Published: July 2024

Endocytosis is a quintessential feature of eukaryotes, and the emergence of endocytosis played a major role in the origin and evolution of eukaryotes. During the early evolution of eukaryotes, phagocytosis and the digestion of prey (i.e., bacteria) combined with the endocytosis of macromolecules opened a new source of nutrients beyond osmotrophy. Pathogenic and commensal protozoa have retained endocytosis as a major mechanism of nutrient acquisition even though, in theory, nutrients could be obtained from the host through osmotrophy. Nearly all pathogenic protozoa exhibit endocytosis and have lysosomal-like compartments that function as digestive vacuoles, and endocytosis appears to play a major role in the acquisition of nutrients. Cryptosporidium is a possible exception that may not exhibit endocytosis. Phagotrophy, however, is only observed in parasites of the intestinal lumen and appears to have been lost in blood and tissue parasites. Overall, the basic features of endocytosis and lysosomes are similar to other eukaryotes. Nonetheless, adaptation to the host has generated some novel features that are specific to certain protozoan lineages.
Host Innate Antiviral Response to Influenza A Virus Infection: From Viral Sensing to Antagonism and Escape
Authors: An Wenlong , Lakhina Simran , Leong Jessica , Rawat Kartik , Husain Matloob
Published: July 2024

Influenza virus possesses an RNA genome of single-stranded, negative-sensed, and segmented configuration. Influenza virus causes an acute respiratory disease, commonly known as the “flu” in humans. In some individuals, flu can lead to pneumonia and acute respiratory distress syndrome. Influenza A virus (IAV) is the most significant because it causes recurring seasonal epidemics, occasional pandemics, and zoonotic outbreaks in human populations, globally. The host innate immune response to IAV infection plays a critical role in sensing, preventing, and clearing the infection as well as in flu disease pathology. Host cells sense IAV infection through multiple receptors and mechanisms, which culminate in the induction of a concerted innate antiviral response and the creation of an antiviral state, which inhibits and clears the infection from host cells. However, IAV antagonizes and escapes many steps of the innate antiviral response by different mechanisms. Herein, we review those host and viral mechanisms. This review covers most aspects of the host innate immune response, i.e., (1) the sensing of incoming virus particles, (2) the activation of downstream innate antiviral signaling pathways, (3) the expression of interferon-stimulated genes, (4) and viral antagonism and escape.
The Effect of KSK-94, a Dual Histamine H3 and Sigma-2 Receptor Ligand, on Adipose Tissue in a Rat Model of Developing Obesity
Authors: Kotańska Magdalena , Zadrożna Monika , Kubacka Monika , Mika Kamil , Szczepańska Katarzyna , Nowak Barbara , Alesci Alessio , Miller Anthea , Lauriano Eugenia Rita , Kieć-Kononowicz Katarzyna
Published: July 2024

Background: Numerous studies highlight the critical role that neural histamine plays in feeding behavior, which is controlled by central histamine H3 and H1 receptors. This is the fundamental motivation for the increased interest in creating histamine H3 receptor antagonists as anti-obesity medications. On the other hand, multiple other neurotransmitter systems have been identified as pharmacotherapeutic targets for obesity, including sigma-2 receptor systems. Interestingly, in our previous studies in the rat excessive eating model, we demonstrated a significant reduction in the development of obesity using dual histamine H3/sigma-2 receptor ligands. Moreover, we showed that compound KSK-94 (structural analog of Abbott’s A-331440) reduced the number of calories consumed, and thus acted as an anorectic compound. Therefore, in this study, we extended the previous research and studied the influence of KSK-94 on adipose tissue collected from animals from our previous experiment. Methods: Visceral adipose tissue was collected from four groups of rats (standard diet + vehicle, palatable diet + vehicle, palatable diet + KSK-94, and palatable diet + bupropion/naltrexone) and subjected to biochemical, histopathological, and immunohistochemical studies. Results: The obtained results clearly indicate that compound KSK-94 prevented the hypertrophy and inflammation of visceral adipose tissue, normalized the levels of leptin, resistin and saved the total reduction capacity of adipose tissue, being more effective than bupropion/naltrexon in these aspects. Moreover, KSK-94 may induce browning of visceral white adipose tissue. Conclusion: Our study suggests that dual compounds with a receptor profile like KSK-94, i.e., targeting histamine H3 receptor and, to a lesser extent, sigma-2 receptor, could be attractive therapeutic options for patients at risk of developing obesity or with obesity and some metabolic disorders. However, more studies are required to determine its safety profile and the exact mechanism of action of KSK-94.
The Gap of Presence: Challenges in Describing Perceptual Phenomena
Authors: Andrade André Dias de
Published: July 2024

This paper reconstructs Merleau-Ponty’s philosophical project in terms of a phenomenology of sensible transcendence. According to this framework, (i) any given data are correlative to a subjective apprehension, (ii) but they cannot be fully captured by this same experience. Therefore, subjective apprehension must remain open to a type of absence or radical indeterminacy. This notion of transcendence must be grounded in bodily experience, and the challenge is to develop a notion of logos that can account for its sensible donation. We describe that the critical apparatus mobilized to achieve this goal, primarily through the notions of “field of presence” and “presentation”, restores a logic of consciousness in these analyses that focus on the body.
Twin Embryos in Arabidopsis thaliana KATANIN 1 Mutants
Authors: Yu Youfeng , Zhu Rui , Xu Hao , Enugutti Balaji , Schneitz Kay , Wang Xuanpeng , Li Jing
Published: July 2024

Regulation of microtubule dynamics is crucial during key developmental transitions such as gametogenesis, fertilization, embryogenesis, and seed formation, where cells undergo rapid changes in shape and function. In plants, katanin plays an essential role in microtubule dynamics. This study investigates two seed developmental mutants in Arabidopsis thaliana, named elk5-1D (erecta-like 5, ELK5) and loo1 (lollipop 1), which are characterized by round seeds, dwarfism, and fertility defects. Notably, elk5-1D exhibits a dominant inheritance pattern, whereas loo1 is recessive. Through positional cloning, we identified both mutants as new alleles of the KATANIN 1 (KTN1) gene, which encodes a microtubule-severing enzyme critical for cell division and morphology. Mutations in KTN1 disrupt embryo cell division and lead to the emergence of a twin embryo phenotype. Our findings underscore the essential role of KTN1 in fertility and early embryonic development, potentially influencing the fate of reproductive cells.
Deciphering High-Temperature-Induced Lignin Biosynthesis in Wheat through Comprehensive Transcriptome Analysis
Authors: Han Junjie , Wang Zhenlong , Wu Xianghu , Xia Jianqiang , Wang Lihong , Wang Zhong , Zhang Yueqiang
Published: July 2024

This study systematically investigated the physiological and molecular responses of the wheat mutant ‘XC-MU201’ under high-temperature stress through comprehensive transcriptome analysis and physiological measurements. RNA sequencing of 21 samples across seven different treatment groups revealed, through Weighted Gene Co-expression Network Analysis (WGCNA), 13 modules among 9071 genes closely related to high-temperature treatments. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses showed significant enrichment of lignin biosynthesis-related modules under high-temperature conditions, especially at the H-10DAT, H-20DAT, and H-30DAT time points. Experimental results demonstrated a significant increase in lignin content in high-temperature-treated samples, confirmed by tissue staining methods, indicating wheat’s adaptation to heat damage through lignin accumulation. The phenylalanine ammonia-lyase gene (TaPAL33) was significantly upregulated under high-temperature stress, peaking at H-30DAT, suggesting its critical role in cellular defense mechanisms. Overexpression of TaPAL33 in the wheat variety ‘Xinchun 11’ enhanced lignin synthesis but inhibited growth. Subcellular localization of GFP-labeled TaPAL33 in tobacco cells showed its distribution mainly in the cytoplasm and cell membrane. Transgenic wheat exhibited higher PAL enzyme activity, enhanced antioxidant defense, and reduced oxidative damage under high-temperature stress, outperforming wild-type wheat. These results highlight TaPAL33’s key role in improving wheat heat tolerance and provide a genetic foundation for future research and applications.
Getting Grip on Phosphorus: Potential of Microalgae as a Vehicle for Sustainable Usage of This Macronutrient
Authors: Solovchenko Alexei , Plouviez Maxence , Khozin-Goldberg Inna
Published: July 2024

Phosphorus (P) is an important and irreplaceable macronutrient. It is central to energy and information storage and exchange in living cells. P is an element with a “broken geochemical cycle” since it lacks abundant volatile compounds capable of closing the P cycle. P fertilizers are critical for global food security, but the reserves of minable P are scarce and non-evenly distributed between countries of the world. Accordingly, the risks of global crisis due to limited access to P reserves are expected to be graver than those entailed by competition for fossil hydrocarbons. Paradoxically, despite the scarcity and value of P reserves, its usage is extremely inefficient: the current waste rate reaches 80% giving rise to a plethora of unwanted consequences such as eutrophication leading to harmful algal blooms. Microalgal biotechnology is a promising solution to tackle this challenge. The proposed review briefly presents the relevant aspects of microalgal P metabolism such as cell P reserve composition and turnover, and the regulation of P uptake kinetics for maximization of P uptake efficiency with a focus on novel knowledge. The multifaceted role of polyPhosphates, the largest cell depot for P, is discussed with emphasis on the P toxicity mediated by short-chain polyPhosphates. Opportunities and hurdles of P bioremoval via P uptake from waste streams with microalgal cultures, either suspended or immobilized, are discussed. Possible avenues of P-rich microalgal biomass such as biofertilizer production or extraction of valuable polyPhosphates and other bioproducts are considered. The review concludes with a comprehensive assessment of the current potential of microalgal biotechnology for ensuring the sustainable usage of phosphorus.
Discrete Element Method Simulation of Particulate Material Fracture Behavior on a Stretchable Single Filter Fiber with Additional Gas Flow
Authors: Asylbekov Ermek , Poggemann Lukas , Dittler Achim , Nirschl Hermann
Published: July 2024

This study presents a comprehensive discrete element method (DEM) simulation approach for the stretching of a filter fiber with a separated polydisperse particle structure on top. For a realistic interaction between the fiber surface and the particles, the original surface of the polymer fiber was projected onto the surface of the fiber cylinder using surface imaging technologies (atomic force microscopy (AFM) and white-light interferometry). In addition, the adhesive forces between particle–fiber and particle–particle contacts were calibrated in the DEM domain using values from self-conducted AFM measurements. Fiber stretching was implemented by the linear motion of small periodic fiber elements. Discretization problems were resolved through studying the stretching of a fiber segment at the size of 8 mm. A critical fiber element length was discovered to be ≈100 μm for minimizing discretization dependencies during the cracking of the particle structure. The number and density of particle–particle contacts within the particle loading on the fiber were obtained at two different elongation rates. Effects such as densification of the particulate structure and increased detachment due to additional air flow were demonstrated.
High-Surface-Area Co-Cu-B Monolithic Self-Supported Catalyst for Efficient Sodium Borohydride Hydrolysis
Authors: Yuan Wuning , Yang Xiao , Liu Cheng , Xue Liangyao , Niu Wenzhe , Yan Qisheng , Zhu Yajie , Han Junchao , Guo Wen , Zhang Bo
Published: July 2024

Sodium borohydride (NaBH4) is a nontoxic and ideal storage material for hydrogen due to its safety and high hydrogen storage capacity. In order to improve the practicality of the sodium borohydride hydrogen production system, we deposited non-precious metal catalytic materials on readily available polymer foams using a simple chemical plating method, developing a suitable 3D catalyst. Its high specific surface area enables it to produce hydrogen at a rate of up to 3.92 L min−1 g−1. Its unique structure gives the catalyst excellent durability. In addition, an efficient NaBH4-based H2 supply system was developed using this catalyst. Co-Cu-B can facilitate stable hydrogen production from NaBH4, yielding a consistent power output ranging from 0 to 100 W. This work provides a new pathway for developing high-efficiency monolithic self-supported catalysts for industrial applications.
Application of Anti-Solvent Crystallization for High-Purity Potash Production from K-Feldspar Leaching Solution
Authors: Shakibania Sina , Sundqvist-Öqvist Lena , Rosenkranz Jan , Ghorbani Yousef
Published: July 2024

Potassium-containing feldspars provide a high potential for producing potash, a product with widespread use in agriculture. The present work assesses applying the anti-solvent crystallization method for the purification and recovery of high-purity muriate of potash (KCl) from feldspar leaching solutions. Initially, screening experiments were carried out on a synthetic leaching solution with the aim of analyzing the crystallization behavior of key components. Screening experiments were performed using five anti-solvents, namely methanol, ethanol, acetone, 2-propanol, and ethylene glycol. Acetone and 2-propanol were viable options for crystallization of potassium chloride. Then, the effects of anti-solvent ratio (O/A), time, and anti-solvent addition rate on potassium-chloride crystallization were further investigated using acetone and 2-propanol. A recovery of 83% of potassium was achieved when using acetone at the O/A of 5 with the addition rate of 10 mL/min, at room temperature with a hold time of 180 min. The optimum conditions for 2-propanol were determined to be similar, except for using a 5 mL/min addition rate for 79% recovery. The final muriate of potash products had a purity of over 99.9% using either of the anti-solvent. However, differences in morphology and crystal size of products were observed. Acetone-formed potash crystals were aggregates of cubic crystals with an average size of 3 microns, while 2-propanol-formed potash crystals were 20 microns in size as cubic particles with a hollow core. Despite having almost the same performance in potassium recovery, acetone was found to be a more feasible anti-solvent for potash recovery due to simpler downstream solvent recovery.
Cold Atmospheric Plasma (CAP) Treatment of In Vitro Cultivated Plum Plantlets—A Possible Way to Improve Growth and Inactivate Plum Pox Virus (PPV)
Authors: Nacheva Lilyana , Milusheva Snezhana , Marinova Plamena , Dimitrova Nataliya , Benova Evgenia
Published: July 2024

Plasma technology, relatively new in the fields of biomedicine, agriculture, and ecology, is the subject of intensive research as a prospective means of decontamination of various microorganisms (bacteria, viruses, and fungi). The objectives of the present study were to follow the effect of cold atmospheric plasma (CAP) treatment on in vitro grown plum plants (Prunus domestica L. ‘Kyustendilska sinya’ cv.) and the possibility of eradicating or inactivating plum pox virus (PPV) causing Sharka disease by CAP. The source tree is naturally co-infected by PPV (both M and D strains). In the experiments, two different plasma sources were used. First, a surface-wave-sustained Argon plasma torch and second, an underwater diaphragm discharge. For the treatments, nodal segments (10 mm in length) from in vitro cultured plum plants with or without one leaf were prepared. Apical shoots from treated plants (PPV-positive and negative clones as well non-treated controls) were cultivated in vitro for four passages. Then they were rooted and acclimatized to ex vitro conditions, and their virus status was observed periodically for more than 3 years after treatment for the appearance of Sharka symptoms. All plants, acclimatized to ex vitro conditions, were tested for PPV by immune capture–reverse transcription–polymerase chain reaction (IC-RT-PCR). As a first step in understanding the plasma treatment of living plants, a plasma treatment variant causing no damage must be established; this has been done in our previous works. Treatment of plants by plasma with parameters that have been carefully selected leads to better development than the non-treated plants. In the treated in vitro plants, no significant differences were found in the number and length of shoots compared to the control plantlets. In ex vitro acclimated plants, greater stem length was reported, but no differences in leaf number were observed. No significant differences in growth were recorded between the control and plants that were treated twice or three times. At this stage, 3 years after ex vitro cultivation in a greenhouse, Sharka symptoms were not registered on treated in vitro negative PPV plants, and the virus was not detected by IC-RT-PCR. Very mild symptoms were showing in CAP-treated PPV-positive plants. Development of typical Sharka symptoms on non-treated controls were observed.
Evaluation of Thermal Insulation of Vacuum-Insulated Casing to Prevent Uncontrollable Melting of Ice and Borehole Instability in Permafrost
Authors: Zhou Xiaohui , Su Yinao , Cheng Yuanfang , Li Qingchao
Published: July 2024

During oil and gas development in permafrost, hot fluids within the wellbore can cause ice melting around wellbore and a decrease in sediment strength, as well as wellbore instability. In the present work, the experimental system for evaluating the insulation effectiveness was established, and the applicability of this experimental system and methodology was verified. It was found that the difference between the experimentally obtained and actual thermal conductivity of the ordinary casings are all within 1.0 W/(m·°C). Meanwhile, the evaluation of insulation effect found that the decrease in fluid temperature, ambient temperature, and vacuum degree can improve its insulation performance. Finally, the numerical simulation was conducted on ice melting and borehole stability during the drilling operation in permafrost. The investigation results demonstrate that the use of vacuum-insulated casings significantly reduces the total heat transferred during the simulation by 86.72% compared to the ordinary casing. The utilization of vacuum-insulated casing reduces the range of ice melting around wellbore to only 16%, which occurs when using ordinary casing. The use of the vacuum-insulated casing resulted in a reduction in the final borehole enlargement rate from 52.1% to 4.2%, and wellbore instability was effectively suppressed.
Impact of Spray Drying on the Properties of Grape Pomace Extract Powder
Authors: Decker Betina Louise Angioletti , Miguel Emilio de Castro , Fonteles Thatyane Vidal , Fernandes Fabiano A. N. , Rodrigues Sueli
Published: July 2024

Incorporating anthocyanins, valuable natural pigments, into a powder can improve their stability, but exposure to high temperatures during processing can cause them to degrade. The purpose of this study was to investigate how the inlet air temperature during spray drying affects the physical and chemical characteristics as well as the flowability of a grape pomace anthocyanin powder obtained through ultrasound-assisted extraction using acidified water as the solvent. An anthocyanin solution containing 13% (w/v) maltodextrin was subjected to spray drying at temperatures ranging from 120 to 170 °C. Tukey’s test was applied to compare the means of the samples. The samples dried at temperatures between 130 and 170 °C were adequate, with a moisture content < 5% and a water activity < 0.3, indicating that the powder was stable. The highest anthocyanin retention (91.94 ± 1.59%) and process yield (50.00 ± 3.06%) were achieved at 140 °C, while higher temperatures resulted in anthocyanin degradation. Furthermore, the powder exhibited poor flowability, indicating cohesive behavior (Hausner ratio > 42.29% and Carr index > 1.73), which is an industrial parameter rarely considered in spray-drying studies. The acidification process was found to promote high anthocyanin retention following high-temperature processing. However, powders obtained from food matrices with low pH and high sugar content may exhibit increased cohesion due to interaction forces. These findings highlight the potential of utilizing grape pomace and green solvents to produce bioactive-rich powders for industrial applications.
Profitability in Public Housing Companies: A Longitudinal and Regional Analysis Using Swedish Panel Data
Authors: Ahmadi Zahra , Berggren Björn , Ismail Mohammad , Silver Lars
Published: July 2024

Public Housing Companies (PHCs) play an important role in the Swedish housing market, with approximately 300 companies managing circa 802,000 dwellings. The public housing sector thereby represents almost 20 percent of the total housing stock in Sweden and half of the apartments that are available for rental. The purpose of this paper is to analyze the most important factors behind the profitability in Swedish PHCs between 2010 and 2019. The effects of internal growth, age, and capital structure in the PHCs are analyzed together with the effect of the growth of the local market, as well as local rent levels. Financial information for circa 300 PHCs in Sweden was gathered from annual reports published between 2010 to 2019. The financial information was analyzed using panel data analysis methods with several explanatory variables to explain the financial performance of the PHCs. The results from the analysis indicate a highly significant and positive relationship between the annual change in population, age, and profitability in the PHC. A highly significant and negative relationship was found between the PHC internal growth, capital structure, and profitability. The results showed no significant relationship between changes in income, rent levels, and profitability in Swedish PHC.
From Medieval Religious Pageantry to Contemporary Social Messaging: The Medieval Cycle Plays in Honduras’ Teatro La Fragua
Authors: De Costa Elena M.
Published: July 2024

Using medieval religious drama as a model, Honduras’ Teatro La Fragua has developed a Gospel dramatization program that both reflects the practices of medieval theater in style and expresses the issues of a modern-day world in message. Their vernacular cycle plays are performed in public spaces by local people, written by and for the community, and staged in the streets and public spaces for ordinary people in both urban and remote rural areas. Medieval vernacular drama thus maintains an enduring stylistic presence in a modern-day counterpart as it underscores the Gospel’s message of inclusion, equity, and diversity while incorporating elements of agency and native culture.
What Kind of God Does Buber’s “I-Thou” Offer to the World: An Introduction to Buber’s Religious Thought
Authors: Kosman Admiel
Published: July 2024

This article has three main goals: (1) To explain in a clear and comprehensible way the difficult basic-word “I-Thou”, which is the basis of Buber’s concept of dialogue, and in fact is the core of his entire teaching (even though it eventually spread over many fields). My main argument in this article is that “I-Thou” is not the “dialogue” that is often spoken of in the name of Buber (not only on the popular level but also in academic circles, and even commonly among those who deal directly with Buber’s teaching) but, rather, that “I-Thou” is a pointing-toward-word—pointing the way for the one whose heart is willing to direct his life to the path of devotion to God—a life whose practical meaning according to Buber is the effort to make room for the presence of the divine (“Shekhinah”) within the stream of earthly normal life, the flow of physical, instinctive life, the flow of life as they are, within “This-World” as it is. (2) This article attempts to follow the sources in Buber’s writings to clearly explain Buber’s faith (which Buber saw as the core of the movement of Hasidism that preceded him). Who is the God that Buber clings to? Why did Buber try to replace the common appellation “God” with a new term of his own: “The Eternal Thou”? (3) It aims to show how the researchers who tried to present Buber as a social or political thinker and removed from his teaching the centrality of his faith entirely distorted his teaching and displaced from it the core of the foundation on which all of Buber’s teaching rests.