bluuebunny/crossref_metadata_2025 huggingface dataset:
Data from Colon Age: A Metric for Whether and How to Screen Male Veterans for Early-Onset Colorectal Cancer
Authors: Imperiale Thomas F. , Myers Laura J. , Barker Barry C. , Stump Timothy E. , Daggy Joanne K.
Published: August 2024

Abstract
We aimed to develop a metric for estimating risk for early-onset colorectal cancer (EOCRC) to help decide whether and how to screen persons < age 50. We used risk prediction models derived and validated on male veterans to calculate the RRs for six scenarios: one low-risk scenario (no risk factors present), four intermediate risk scenarios (some risk factors present), and one high-risk scenario (all risk factors present) for three age groups (35–39, 40–44, and 45–49 years). For each scenario, we estimated absolute colorectal cancer risk using Surveillance Epidemiology and End Results colorectal cancer incidence rates and each scenario’s RR. We identified the current Surveillance Epidemiology and End Results 5-year age group to which the revised estimate was closest and refer to the midpoint of this group as the “colon age.” When the revised estimate equals or exceeds that for 50- to 54-year-olds and for 70- to 74-year-olds, respective recommendations were made for (any) colorectal cancer screening and screening with colonoscopy. Among the scenarios, there was inconsistency between the two models for the 35 to 39 and 40 to 44 age groups, with only the 15-variable model recommending screening for the higher-risk 35- to 39-year-olds. Both models recommended screening for some intermediate risk and high-risk 40- to 44-year-olds. The models were well aligned on whether and how to screen most 45- to 49-year-olds. Using risk factors for EOCRC with colorectal cancer incidence rates, “colon age” may be useful for shared decision-making about whether and how to screen male veterans <50 years. For 45- to 49-year-olds, the 7-variable model may be preferred by patients, providers, and health systems.

Prevention Relevance: A new metric known as “colon age” expresses risk of EOCRC based on biological risk and may be useful for providers to explain and for patients to understand colorectal cancer risk when considering whether and how to be screened for colorectal cancer prior to age 45 or 50.
Supplementary Figure S5 from CLN-617 Retains IL2 and IL12 in Injected Tumors to Drive Robust and Systemic Immune-Mediated Antitumor Activity
Authors: Mehta Naveen K. , Rakhra Kavya , Meetze Kristan A. , Li Bochong , Momin Noor , Chang Jason Y.H. , Wittrup K. Dane , Baeuerle Patrick A. , Michaelson Jennifer S.
Published: August 2024

Dose response in the B16F10 model
Supplementary Figure S4 from CLN-617 Retains IL2 and IL12 in Injected Tumors to Drive Robust and Systemic Immune-Mediated Antitumor Activity
Authors: Mehta Naveen K. , Rakhra Kavya , Meetze Kristan A. , Li Bochong , Momin Noor , Chang Jason Y.H. , Wittrup K. Dane , Baeuerle Patrick A. , Michaelson Jennifer S.
Published: August 2024

Dose level and schedule evaluation for the treatment of CT26 tumors
Supplementary Figure S15 from CLN-617 Retains IL2 and IL12 in Injected Tumors to Drive Robust and Systemic Immune-Mediated Antitumor Activity
Authors: Mehta Naveen K. , Rakhra Kavya , Meetze Kristan A. , Li Bochong , Momin Noor , Chang Jason Y.H. , Wittrup K. Dane , Baeuerle Patrick A. , Michaelson Jennifer S.
Published: August 2024

Activity of CLN-617 on PBMCs
Supplementary Figure 9 from Multifunctional Bispecific Nanovesicles Targeting SLAMF7 Trigger Potent Antitumor Immunity
Authors: Zhu Manman , Wu Yongjian , Zhu Tianchuan , Chen Jian , Chen Zhenxing , Ding Hanxi , Tan Siyi , He Jianzhong , Zeng Qi , Huang Xi
Published: August 2024

Supplementary Figure 9
Supplementary Figure 6 from Multifunctional Bispecific Nanovesicles Targeting SLAMF7 Trigger Potent Antitumor Immunity
Authors: Zhu Manman , Wu Yongjian , Zhu Tianchuan , Chen Jian , Chen Zhenxing , Ding Hanxi , Tan Siyi , He Jianzhong , Zeng Qi , Huang Xi
Published: August 2024

Supplementary Figure 6
Supplementary Figure 17 from Multifunctional Bispecific Nanovesicles Targeting SLAMF7 Trigger Potent Antitumor Immunity
Authors: Zhu Manman , Wu Yongjian , Zhu Tianchuan , Chen Jian , Chen Zhenxing , Ding Hanxi , Tan Siyi , He Jianzhong , Zeng Qi , Huang Xi
Published: August 2024

Supplementary Figure 17
Supplementary Figure 12 from Multifunctional Bispecific Nanovesicles Targeting SLAMF7 Trigger Potent Antitumor Immunity
Authors: Zhu Manman , Wu Yongjian , Zhu Tianchuan , Chen Jian , Chen Zhenxing , Ding Hanxi , Tan Siyi , He Jianzhong , Zeng Qi , Huang Xi
Published: August 2024

Supplementary Figure 12
Supplementary Figure 10 from Multifunctional Bispecific Nanovesicles Targeting SLAMF7 Trigger Potent Antitumor Immunity
Authors: Zhu Manman , Wu Yongjian , Zhu Tianchuan , Chen Jian , Chen Zhenxing , Ding Hanxi , Tan Siyi , He Jianzhong , Zeng Qi , Huang Xi
Published: August 2024

Supplementary Figure 10
Supplementary Table S2 from Venetoclax Induces BCL-2–Dependent Treg to TH17 Plasticity to Enhance the Antitumor Efficacy of Anti–PD-1 Checkpoint Blockade
Authors: Liao Rosy , Hsu Jocelyn Y. , Aboelella Nada S. , McKeever Joshua A. , Thomas-Toth Anika T. , Koh Andrew S. , LaBelle James L.
Published: August 2024

Supplementary Table S2
Figure S4 from Impact of scFv on Functionality and Safety of Third-Generation CD123 CAR T Cells
Authors: Fredon Maxime , Poussard Margaux , Biichlé Sabeha , Bonnefoy Francis , Mantion Charles-Frédéric , Seffar Evan , Renosi Florian , Bôle-Richard Elodie , Boidot Romain , Chevrier Sandrine , Anna François , Loustau Maria , Caumartin Julien , Gonçalves-Venturelli Mathieu , Robinet Eric , Saas Philippe , Deconinck Eric , Daguidau Etienne , Roussel Xavier , Godet Yann , Adotévi Olivier , Angelot-Delettre Fanny , Galaine Jeanne , Garnache-Ottou Francine
Published: August 2024

Supplementary Figure S4 Effect of CD123 CAR-T on humanized mouse model, NSG-SGM3.
Supplementary Figure S4 from Overexpression of an Engineered SERPINB9 Enhances Allogeneic T-cell Persistence and Efficacy
Authors: Teo Pei Y. , Jung Youngrock , Quach David H. , Koh Joanna , Ong Richard W. , Goh Angeline , Tan Alrina , Ng Chee H. , Seh Cheah C. , Tan Kar W. , Horak Ivan D. , Low Lionel
Published: August 2024

Supplementary Figure S4. CD30.CAR with 4-1BB spacer (CD30.bb) EBVSTs expressing different forms of SB9 had similar CD30.bb expression and T cell characteristics. (A, left) SDS-PAGE analysis of SB9 expression from a representative donor, where the top band (∼62 kDa) indicates the pre-formed GzmB and SB9 complexes in the cytosol and the bottom band (∼43 kDa) indicates the functional SB9 monomer. Transduction was carried out in the presence of IL-7 (10 ng/mL) and either low (10 ng/mL) or high (100 ng/mL) IL-15 concentration, as denoted by ‘L’ and ‘H’ respectively. (A, right) Densitometry analysis of SB9 monomer normalized to CD30.bb EBVST control and calnexin as a loading control under condition ‘L’ (IL-7 and IL-15 at 10 ng/mL). (B, left) CD30.bb expression on EBVSTs expressing different variants of SB9, measured by flow cytometry analysis of a representative donor. Pooled data of CD30.bb expression (%; B, middle) and CD30.bb expression (geometric mean fluorescence intensity, MFI; B, right) on CD30.bb EBVSTs, measured by flow cytometry analysis of n = 4 donors, where bars denote median ± SD. (C) Background subtracted percentage of CD3-positive T cells producing IFNγ and/or TNFα in response to EBV peptides, analyzed by intracellular cytokine staining and flow cytometry, where the bars represent the mean ± SD (n = 4 donors). Background was determined by measuring percentage of CD3-positive T cells producing IFNγ and/or TNFα in response to HIV peptides. D-H, Flow cytometry analysis of CD4/CD8 subsets (%) (D), memory T cell subsets (%), where CM: central memory, EM: effector memory, and TEMRA: T effector memory expressing CD45RA (E), CD39 expression (%) (F), CD25 expression (%) (G, left) and geometric mean fluorescence intensity (MFI) (G, right), and PD-1, TIM-3, LAG-3 expression (H), of CD30.bb EBVSTs from a representative donor.
Table S14 from Improvement of Tumor Neoantigen Detection by High-Field Asymmetric Waveform Ion Mobility Mass Spectrometry
Authors: Meng Wei , Takeuchi Yoshiko , Ward Jeffrey P. , Sultan Hussein , Arthur Cora D. , Mardis Elaine R. , Artyomov Maxim N. , Lichti Cheryl F. , Schreiber Robert D.
Published: August 2024

Top 33 predicted F244 MHC-I neoepitopes.
Table S1 from Improvement of Tumor Neoantigen Detection by High-Field Asymmetric Waveform Ion Mobility Mass Spectrometry
Authors: Meng Wei , Takeuchi Yoshiko , Ward Jeffrey P. , Sultan Hussein , Arthur Cora D. , Mardis Elaine R. , Artyomov Maxim N. , Lichti Cheryl F. , Schreiber Robert D.
Published: August 2024

Peptide inclusion list for PRM. Related to “Mass Spectrometry by PRM” in Methods.
Figure S7 from Improvement of Tumor Neoantigen Detection by High-Field Asymmetric Waveform Ion Mobility Mass Spectrometry
Authors: Meng Wei , Takeuchi Yoshiko , Ward Jeffrey P. , Sultan Hussein , Arthur Cora D. , Mardis Elaine R. , Artyomov Maxim N. , Lichti Cheryl F. , Schreiber Robert D.
Published: August 2024

F244 MHC-I and -II neoantigen prediction and screens.
Figure S5 from Improvement of Tumor Neoantigen Detection by High-Field Asymmetric Waveform Ion Mobility Mass Spectrometry
Authors: Meng Wei , Takeuchi Yoshiko , Ward Jeffrey P. , Sultan Hussein , Arthur Cora D. , Mardis Elaine R. , Artyomov Maxim N. , Lichti Cheryl F. , Schreiber Robert D.
Published: August 2024

1956 MHC-I and -II neoantigen prediction and screens.
Supplementary Figure S7 from mTOR Signaling Promotes Rapid m6A mRNA Methylation to Regulate NK-Cell Activation and Effector Functions
Authors: Meng Meng , Zhong Zhaoyang , Song Liang , Zhang Zhaohui , Yin Xiaofeng , Xie Xiqiang , Tian Lei , Wu Wei , Yang Yao , Deng Yafei , Peng Hongyan , Wu Shuting , Ran Guanghe , Lin Yuqing , Lai Qiangqiang , Bi Qinghua , Yan Fulin , Ji Yan , Wang Yang , Li Xiaohui , Yi Ping , Yu Jianhua , Deng Youcai
Published: August 2024

Supplementary Figure S7
Supplemental Figure 2 from T-Cell Expression of CXCL13 is Associated with Immunotherapy Response in a Sex-Dependent Manner in Patients with Lung Cancer
Authors: Brennan Michelle , DeBruin David , Nwokolo Chinye , Hunt Katey S. , Piening Alexander , Donlin Maureen J. , Ferris Stephen T. , Teague Ryan M. , DiPaolo Richard J. , Alspach Elise
Published: August 2024

Supplemental Figure 2: Sex-based differences in CXCL13 expression level and frequency of CXCL13+ T cells.
Supplementary Figure S7 from Low Serum Apolipoprotein A1 Levels Impair Antitumor Immunity of CD8+ T Cells via the HIF-1α–Glycolysis Pathway
Authors: Lv Qiaoying , Su Tong , Liu Wei , Wang Lulu , Hu Jiali , Cheng Yali , Ning Chengcheng , Shan Weiwei , Luo Xuezhen , Chen Xiaojun
Published: August 2024

Supplementary Figure S7. Anti-PD-1 therapy has no antitumor effects in tumor-bearing A1KO mice (related to Figure 7)
Supplementary Figure S6 from Low Serum Apolipoprotein A1 Levels Impair Antitumor Immunity of CD8+ T Cells via the HIF-1α–Glycolysis Pathway
Authors: Lv Qiaoying , Su Tong , Liu Wei , Wang Lulu , Hu Jiali , Cheng Yali , Ning Chengcheng , Shan Weiwei , Luo Xuezhen , Chen Xiaojun
Published: August 2024

Figure S6. ApoA1 stabilizes HIF1α protein by reducing HIF1α protein ubiquitination (related to Figure 6)
Supplementary Figure S4 from Low Serum Apolipoprotein A1 Levels Impair Antitumor Immunity of CD8+ T Cells via the HIF-1α–Glycolysis Pathway
Authors: Lv Qiaoying , Su Tong , Liu Wei , Wang Lulu , Hu Jiali , Cheng Yali , Ning Chengcheng , Shan Weiwei , Luo Xuezhen , Chen Xiaojun
Published: August 2024

Supplementary Figure S4. ApoA1 mimetic peptide L-4F potentiates the antitumor effect of CD8+ T cells (related to Figure 4)
Data from Improvement of Tumor Neoantigen Detection by High-Field Asymmetric Waveform Ion Mobility Mass Spectrometry
Authors: Meng Wei , Takeuchi Yoshiko , Ward Jeffrey P. , Sultan Hussein , Arthur Cora D. , Mardis Elaine R. , Artyomov Maxim N. , Lichti Cheryl F. , Schreiber Robert D.
Published: August 2024

Abstract
Cancer neoantigens have been shown to elicit cancer-specific T-cell responses and have garnered much attention for their roles in both spontaneous and therapeutically induced antitumor responses. Mass spectrometry (MS) profiling of tumor immunopeptidomes has been used, in part, to identify MHC-bound mutant neoantigen ligands. However, under standard conditions, MS-based detection of such rare but clinically relevant neoantigens is relatively insensitive, requiring 300 million cells or more. Here, to quantitatively define the minimum detectable amounts of therapeutically relevant MHC-I and MHC-II neoantigen peptides, we analyzed different dilutions of immunopeptidomes isolated from the well-characterized T3 mouse methylcholanthrene (MCA)-induced cell line by MS. Using either data-dependent acquisition or parallel reaction monitoring (PRM), we established the minimum amount of material required to detect the major T3 neoantigens in the presence or absence of high field asymmetric waveform ion mobility spectrometry (FAIMS). This analysis yielded a 14-fold enhancement of sensitivity in detecting the major T3 MHC-I neoantigen (mLama4) with FAIMS-PRM compared with PRM without FAIMS, allowing ex vivo detection of this neoantigen from an individual 100 mg T3 tumor. These findings were then extended to two other independent MCA-sarcoma lines (1956 and F244). This study demonstrates that FAIMS substantially increases the sensitivity of MS-based characterization of validated neoantigens from tumors.
Consolidation of Aniops Casey, Psiona Casey, Preglyptobaris Bondar and Prospoliata Hustache with Chryasus Champion (Coleoptera, Curculionidae, Baridinae) and descriptions of new species
Authors: PRENA JENS
Published: August 2024

Aniops Casey, 1922, Psiona Casey, 1922, Preglyptobaris Bondar, 1946 and Prospoliata Hustache, 1950 are here proposed as new junior synonyms of Chryasus Champion, 1908 because their ostensibly diagnostic character states are more or less continuous following examination of numerous species from throughout their geographic ranges and are not valid as separate lineages in this broader context. Amphibaris bruniceps Bondar, 1946, Aniops sculpturatus Casey, 1922, Baris multistriata Chevrolat, 1880, Preglyptobaris bicolor Bondar, 1946, Psiona carinulosa Casey, 1922 and Psiona densa Casey, 1922 are transferred to Chryasus (new combinations). Baridius ignavus Boheman, 1844 and Amphibaris dispar Bondar, 1946 are transferred from Sphenobaris Champion, 1909 to Glyptobaris Casey, 1892 (new combinations). Prospoliata bicolorata Hustache, 1950 is a new junior synonym of Preglyptobaris bicolor Bondar, 1946 and Psiona opaca Casey, 1922 is a new junior synonym of Psiona densa Casey, 1922. Ten new species are described: C. bicuspis Prena new species (Ecuador), C. clunis Prena new species, C. macer Prena new species, C. montyi Prena new species, C. nimius Prena new species (all Costa Rica), C. persimilis Prena new species (Costa Rica, Panamá), C. pomponella Prena new species (Perú), C. taironius Prena new species (Colombia), C. talamanca Prena new species (Panamá) and C. wappesi Prena new species (French Guiana, Venezuela). These actions increase the number of included species from two to eighteen. They occur from México to Brazil and in the Lesser Antilles. Two species induce and develop in elongate galls in leaf petioles of Miconia pittieri Cogn. and M. prasina (Sw.) DC (Melastomataceae), respectively. Several others with unknown biology were collected from the same plant genus or unidentified Melastomataceae.
A review of the genus Neoconger (Anguilliformes: Moringuidae), with the description of a new species
Authors: SMITH DAVID G. , MARCENIUK ALEXANDRE P. , ROTUNDO MATHEUS M. , CARVALHO CINTIA O. , CAIRES RODRIGO A.
Published: August 2024

The genus Neoconger (family Moringuidae) is reviewed and our knowledge of it brought up to date. Six species are recognized, based on genetic and morphological information. Neoconger mucronatus Girard, 1858 occurs in the western Gulf of Mexico. Neoconger torrei (Howell Rivero, 1932) occurs in the Caribbean and along the coast of the Guianas. Neoconger anaelisae (Tommasi, 1960) occurs along the northern coast of Brazil. Neoconger hygomi n. sp. is described from a single specimen collected many years ago off Brazil’s southern coast. It is distinguished from the other species by the number of predorsal vertebrae (48 vs 32–45) and preanal vertebrae (55 vs 41–49). Neoconger vermiformis Gilbert, 1890 occurs in the eastern Pacific from the northern Gulf of California to Panama and northern Colombia. Neoconger tuberculatus (Castle, 1965) is known from two larvae collected off southeastern Australia. In addition, another species known only from larvae is reported from the Caribbean but is not named.
Evaluate a comprehensive geriatric assessment service framework targeting frail older people who had high risk of requiring long-term care services in Japan: a community-based pilot study
Authors: Sato Kanae , Huq K. A. T. M. Ehsanul , Kazawa Kana , Kawai Madoka , Moriyama Michiko
Published: August 2024

Background

Frailty has become a key concern in an aging population. A comprehensive geriatric assessment (CGA) service framework was developed and evaluated aiming to target and connect frail older adults who are at high risk of requiring long-term care services.

Methods

A community-based pilot study was conducted in fiscal year 2016 and 2017 in Kure city, Hiroshima, Japan. Participants aged 65 and over living in Kure city, and 393 persons were extracted from the Kihon Check List (KCL) responses. Among the eligible individuals, 101 consented to participate and received CGA and referred to services based on individual health needs. The efficacy was evaluated by referral rate of services, continuity of the service usage, evaluation of participant’s health condition and the quality of life (QoL) after the 6-month follow-up.

Results

Ninety-nine (98.0%) participants needed support for the instrumental activity of daily living, 97 (96.0%) were categorized as locomotive syndrome, and 64 (63.4%) had a depressive tendency. Afterward, 60 participants (59.4%) subsequently accepted the referral services, however, 34 (33.7%) used the services and the remaining 26 (25.7%) did not use the services. The health condition improvements in the service-uses group were statistically significant (p < 0.001), however, QoL score did not change between the baseline and 6th -month.

Conclusion

KCL extracted high-risks older people, and CGA revealed related diseases and health conditions. However, the high refusal rate of referral services indicates a necessity to modify the service framework such as by collaborating with community general support centers, which could increase the efficacy of service framework.
Kidney and cardiovascular-protective benefits of combination drug therapies in chronic kidney disease associated with type 2 diabetes
Authors: Khan Muhammad Shahzeb , Lea Janice P.
Published: August 2024

Given the substantial burden of chronic kidney disease associated with type 2 diabetes, an aggressive approach to treatment is required. Despite the benefits of guideline-directed therapy, there remains a high residual risk of continuing progression of chronic kidney disease and of cardiovascular events. Historically, a linear approach to pharmacologic management of chronic kidney disease has been used, in which drugs are added, then adjusted, optimized, or stopped in a stepwise manner based on their efficacy, toxicity, effects on a patient’s quality of life, and cost. However, there are disadvantages to this approach, which may result in missing a window of opportunity to slow chronic kidney disease progression. Instead, a pillar approach has been proposed to enable earlier treatment that simultaneously targets multiple pathways involved in disease progression. Combination therapy in patients with chronic kidney disease associated with type 2 diabetes is being investigated in several clinical trials. In this article, we discuss current treatment options for patients with chronic kidney disease associated with type 2 diabetes and provide a rationale for tailored combinations of therapies with complementary mechanisms of action to optimize therapy using a pillar-based treatment strategy. [This article includes a plain language summary as an additional file].
Magnesium sulfate for fetal neuroprotection in preterm pregnancy: a meta-analysis of randomized controlled trials
Authors: Jafarabady Kyana , Shafiee Arman , Eshraghi Nasim , Salehi Seyyed Amirhossein , Mohammadi Ida , Rajai Shahryar , Zareian Zahra , Movahed Fatemeh , Bakhtiyari Mahmood
Published: August 2024

Background

Intravenous administration of magnesium sulfate (MgSO4) to expectant individuals before childbirth, has been evaluated to reduce the likelihood of mortality and occurrence cerebral palsy in their offspring. Therefore, this systematic review and meta-analysis conducted to determine if were the prophylactic use of magnesium sulfate in women at risk for preterm delivery leads to decrease in the incidence of death or cerebral palsy.

Methods

A comprehensive search of electronic databases was done to identify relevant studies. Selection of eligible studies was based on predetermined inclusion criteria. Data extraction was performed, and the methodological quality of the selected studies was assessed using appropriate evaluative tools. A meta-analysis was carried out to estimate the overall effect of intravenous administration of magnesium sulfate on the incidence of death or cerebral palsy.

Results

A total of 7 studies met the inclusion criteria and were included in the final analysis. No significant publication bias was observed. The risk of fetal neurological impairment was significantly lower in the MgSO4 group compared to the control group relative risk (RR = 0.70, 95% CI: 0.56 to 0.87; I20%). However, neonatal mortality was not significantly associated with MgSO4 injection. (RR = 1.03, 95% CI: 0.88 to 1.21; I2 = 42%). Subgroup analysis was done based on the bolus dosage of MgSO4 and the duration of the trial follow-up. revealing a non-significant differences between-group.

Conclusion

This study demonstrated that MgSO4 administration can improve fetal neurological impairment and cerebral palsy but is not linked to reducing mortality. Further studies are necessary to strengthen the evidence and clarify the underlying mechanisms.
Maternal hypothyroidism and subsequent metabolic outcomes in children: a systematic review and meta-analysis
Authors: Zhao Lucy , Selvaratnam Inthuja , Cunningham Jessie , Filion Kristian B. , Grandi Sonia M.
Published: August 2024

Introduction

As the fetus relies on maternal thyroid hormones in early pregnancy, maternal hypothyroidism plays an important role in fetal development. However, the association between maternal hypothyroidism and metabolic disease in offspring is unclear.

Objective

To examine the association between maternal hypothyroidism in pregnancy and metabolic outcomes (obesity, hypertension, type 2 diabetes mellitus, and dyslipidemia) in children < 18 years.

Methods

We systematically searched 5 databases from inception to May 2023. Eligible studies included cohort, case-control, and randomized controlled trials involving children born to mothers with or without hypothyroidism in pregnancy. Data were pooled across studies using random-effects models for outcomes reported in at least three studies. Quality assessment was performed using the ROBINS-E tool for observational studies and the Cochrane Risk of Bias tool for trials.

Results

The search identified 3221 articles, of which 7 studies were included (1 trial, 6 observational). All studies were conducted outside of North America and ranged in size from 250 to > 1 million children. The follow-up time ranged from 6 to 20 years. Included studies support an increased risk of hypertension and glucose dysregulation in offspring exposed to maternal hypothyroidism (hypertension: OR 1.08, 95% CI 0.75, 1.57 and HR 1.81, 95% CI 1.21, 2.69; diabetes: RR 2.7, 95% CI 0.7, 10). In the pooled analysis, maternal hypothyroidism was not associated with obesity in offspring (OR 1.04, 95% CI 0.64, 1.70).

Conclusion

This study found inconsistent evidence on the association between maternal hypothyroidism in pregnancy and metabolic outcomes in offspring, though associations with hypertension and glucose dysregulation are possible.
Predictors for acute exacerbation of interstitial pneumonia following lung cancer surgery: a multicenter study
Authors: Yin Lijie , Xu Rui , Liu Xiaojian , Fu Liping , Qiang Guangliang
Published: August 2024

Background

Acute exacerbation (AE) of interstitial lung disease (ILD) is one of the most serious complications during perioperative period of lung cancer resection. This study aimed to investigate the correlation between preoperative 2- deoxy-2-[18F]fluoro-D-glucose (18F-FDG) PET/CT findings and AE in lung cancer patients with ILD.

Methods

We retrospectively reviewed the data of 210 patients who underwent lung resection for non-small cell lung cancer. Relationships between clinical data and PET images and AE were evaluated. The patients were divided into an AE(+) and an AE(-) group for multivariate logistic regression analysis. Receiver operating characteristic (ROC) curve analysis was conducted and the area under curve (AUC) was used to assess the predictive values.

Results

Among 210 patients, 48 (22.8%) were diagnosed with ILD based on chest CT. Among them, 9 patients (18.75%) developed AE after lung resection and were defined as AE(+) group. The course of ILD was longer in AE(+) group compared to AE(-) group. More patients in AE(+) group had a history of AE and chronic obstructive pulmonary disease (COPD) than in AE(-) group. The maximum standardized uptake value (SUVmax) of the noncancerous interstitial pneumonia (IP) area and cancers in AE(+) group was significantly higher compared to AE(-) group. Univariate logistic regression analysis showed that AE, COPD, SUVmax of the noncancerous IP area, SUVmax of cancer, surgical method were significantly correlated with AE. The course of ILD[OR(95%CI) 2.919; P = 0.032], SUVmax of the noncancerous IP area[OR(95%CI) 7.630;P = 0.012] and D-Dimer level[OR(95%CI) 38.39;P = 0.041] were identified as independent predictors for AE in patients with ILD after lung cancer surgery. When the three indicators were combined, we found significantly better predictive performance for postoperative AE than that of SUVmax of the noncancerous IP area alone [0.963 (95% CI 0.914-1.00); sensitivity, 100%, specificity 87.2%, P < 0.001 vs. 0.875 (95% CI 0.789 ~ 0.960); sensitivity, 88.9%, specificity, 76.9%, P = 0.001; difference in AUC = 0.088, Z = 1.987, P = 0.04].

Conclusion

The combination of the course of ILD, SUVmax of the noncancerous IP area and D-Dimer levels has high predictive value for the occurrence of AE in patients with concomitant interstitial lesions.
Arthroscopic assisted versus open non-vascularized bone grafting in delayed union and nonunion of the scaphoid: a systematic review and meta-analysis
Authors: Noori Atefeh , Persitz Jonathan , Khan Shawn , Chan Andrea , Paul Ryan
Published: August 2024

Background

Delayed union and nonunion of the scaphoid is a common complication often requiring surgical reconstruction and bone grafting. Our goal was to systematically assess the healing time and clinical outcomes following arthroscopic-assisted versus open non-vascularized bone grafting of the scaphoid.

Methods

A comprehensive search of the MEDLINE, Embase, CINAHL (Cumulative Index to Nursing and Allied Health Literature), and Cochrane Central databases was completed from inception to September 2023. We included randomized trials and observational studies that reported outcomes following scaphoid delayed union/nonunion comparing arthroscopic-assisted vs. open non-vascularized bone grafting. Two reviewers independently extracted data and assessed the risk of bias. One investigator assessed certainty of evidence and a senior investigator confirmed the assessment. We pooled effects using random-effects models, when possible, for all outcomes reported by more than 1 study.

Results

Overall, 26 studies and 822 patients were included in the study. Very low certainty evidence demonstrated that arthroscopic-assisted surgery may decrease healing time compared to open surgery (weighted mean difference [WMD] -7.8 weeks; 95%CI -12.8 to -2.8). Arthroscopic bone grafting did not result in an improvement in union rate (relative risk 1.01; 95%CI 0.9 to 1.09). The pooled data in arthroscopic graft group showed mean time to union of 11.4 weeks (95%CI: 10.4 to 12.5) with union rate of 95% (95%CI 91–98%). A single comparative study reported very low certainty evidence that arthroscopy-assisted vs. open surgery may not have an effect on pain relief (MD 0 cm, 95%CI -0.4 to 0.5 on VAS 10 cm for pain) or improving function (MD -1.2, 95% CI -4.8 to 2.3 on 100 points DASH).

Conclusion and future directions

Our results suggest that arthroscopic-assisted non-vascularized bone grafting may be associated with improved average weeks to heal in comparison with open surgery for scaphoid delayed union/nonunion reconstruction with overall comparable union rates. There is insufficient evidence to assess the effects of arthroscopic-assisted reconstruction on union rate, time to union, and patient-reported outcomes in patients with other important nonunion characteristics such as established humpback deformity.
Associations between lifestyle-related risk factors and back pain: a systematic review and meta-analysis of Mendelian randomization studies
Authors: Guan Jianbin , Liu Tao , Gao Ge , Yang Kaitan , Liang Haohao
Published: August 2024

Background

Mendelian randomization (MR) studies have an advantage over conventional observational studies when studying the causal effect of lifestyle-related risk factors on back pain. However, given the heterogeneous design of existing MR studies on back pain, the reported causal estimates of these effects remain equivocal, thus obscuring the true extent of the biological effects of back pain lifestyle-risk factors.

Purpose

The purpose of this study was to conduct a systematic review with multiple meta-analyses on the associations between various lifestyle factors and low back pain.

Methods

We conducted a PRISMA systematic review and specifically included MR studies to investigate the associations between lifestyle factors—specifically, BMI, insomnia, smoking, alcohol consumption, and leisure sedentary behavior—and various back pain outcomes. Each meta-analysis synthesized data from three or more studies to assess the causal impact of these exposures on distinct back pain outcomes, including chronic pain, disability, and pain severity. Quality of studies was assessed according to STROBE-MR guidelines.

Results

A total of 1576 studies were evaluated and 20 were included. Overall, the studies included were of high quality and had a low risk of bias. Our meta-analysis demonstrates the positive causal effect of BMI (OR IVW−random effects models: 1.18 [1.08–1.30]), insomnia(OR IVW−random effects models: 1.38 [1.10–1.74]), smoking(OR IVW−fixed effects models: 1.30 [1.23–1.36]), alcohol consumption(OR IVW−fixed effects models: 1.31 [1.21–1.42]) and leisure sedentary behaviors(OR IVW−random effects models: 1.52 [1.02–2.25]) on back pain.

Conclusion

In light of the disparate designs and causal effect estimates presented in numerous MR studies, our meta-analysis establishes a compelling argument that lifestyle-related risk factors such as BMI, insomnia, smoking, alcohol consumption, and leisure sedentary behaviors genuinely contribute to the biological development of back pain.
A larger radius of the medial femoral posterior condyle is a risk factor for medial meniscus posterior root tears
Authors: Wang Junsen , Peng Kangzu , Gu Juyuan , Gao Shijun
Published: August 2024

Background

Studies have shown an association between medial meniscus posterior root tears (MMPRT) and morphologic characteristics of the bone. However, the association between distal femoral bone morphology and MMPRT, particularly the medial femoral posterior condyle, is poorly understood. Our study aimed to determine the association between the morphologic characteristics of the medial posterior femoral condyle and MMPRT.

Methods

A retrospective case-control study was performed from January 2021 to January 2022. After screening based on the inclusion and exclusion criteria, two matched groups were analyzed: the MMPRT group and the isolated lateral meniscus tears group. The hip-knee-ankle angle (HKA) and Kellgren-Lawrence grade (KLG) were measured on radiographs; the medial tibial slope angle (MTSA), medial tibial plateau depth (MTPD), and radius of the medial femoral posterior condyle (RMFPC) were measured on magnetic resonance imaging (MRI) in both groups. The area under the curve (AUC) and the best cutoff value for predicting MMPRT were calculated by using receiver operating characteristic (ROC) curve analysis.

Results

The final analysis included a total of 174 patients (87 MMPRT patients and 87 controls). Significant differences were shown in the RMFPC (17.6 ± 1.0 vs. 16.2 ± 1.0, p < 0.01) and MTSA (6.4 ± 2.0 vs. 4.0 ± 1.3, p < 0.01), which were larger than those of the control group. The MTPD (1.8 ± 0.6 vs. 2.9 ± 0.7, p < 0.01) and HKA (175.4 ± 2.2 vs. 179.0 ± 2.7, p < 0.01) of the injury group were significantly different from the control group, and both were lower than the control group. However, between the MMPRT and control groups on the KLG (2.3 ± 0.6 vs. 2.2 ± 0.6, p = 0.209), there was no statistically significant difference. Among them, the RMFPC cutoff value was calculated to be 16.8 mm by ROC curve analysis, and the sensitivity and specificity were both 81.61%.

Conclusions

This study demonstrated that larger RMFPC, MTSA, smaller MTPD, and HKA were all associated with MMPRT, and RMFPC ≥ 16.8 mm was considered as a significant risk factor for MMPRT.
Unveiling potential: urinary exosomal mRNAs as non-invasive biomarkers for early prostate cancer diagnosis
Authors: Yu Jiayin , Yu Chifei , Jiang Kangxian , Yang Guanglin , Yang Shubo , Tan Shuting , Li Tingting , Liang Haiqi , He Qihuan , Wei Faye , Li Yujian , Cheng Jiwen , Wang Fubo
Published: August 2024

Background

This study investigated the use of urinary exosomal mRNA as a potential biomarker for the early detection of prostate cancer (PCa).

Methods

Next-generation sequencing was utilized to analyze exosomal RNA from 10 individuals with confirmed PCa and 10 individuals without cancer. Subsequent validation through qRT-PCR in a larger sample of 43 PCa patients and 92 healthy controls revealed distinct mRNA signatures associated with PCa.

Results

Notably, mRNAs for RAB5B, WWP1, HIST2H2BF, ZFY, MARK2, PASK, RBM10, and NRSN2 showed promise as diagnostic markers, with AUC values between 0.799 and 0.906 and significance p values. Combining RAB5B and WWP1 in an exoRNA diagnostic model outperformed traditional PSA tests, achieving an AUC of 0.923, 81.4% sensitivity, and 89.1% specificity.

Conclusions

These findings highlight the potential of urinary exosomal mRNA profiling, particularly focusing on RAB5B and WWP1, as a valuable strategy for improving the early detection of PCa.
METS-IR/HOMA-IR and MAFLD in U.S. adults: dose–response correlation and the effect mediated by physical activity
Authors: Peng Hongye , Xiang Jingjing , Pan Liang , Zhao Mo , Chen Bin , Huang Shuxia , Yao Ziang , Liu Jing , Lv Wenliang
Published: August 2024

Objectives

Metabolic dysfunction-associated fatty liver disease (MAFLD), a globally prevalent disease, is closely linked to insulin resistance (IR). Physical activity (PA) is closely linked to both MAFLD and IR. We aim to explore the dose–response relationship between metabolic score for IR (METS-IR)/homeostasis model assessment of IR (HOMA-IR) and MAFLD, and investigate the relationship between PA, IR and MAFLD.

Methods

Participants from the NHANES study were included in this cross-section study. Logistic regression and the receiver operating characteristic were used to assess the predictive performance of METS-IR/HOMA-IR for MAFLD. Restrictive cubic splines were performed to visualize their dose–response relationship. Decision tree analysis was used to identify high-risk populations of MAFLD. PA’s mediating effect in the association between METS-IR/HOMA-IR and MAFLD was also examined.

Results

Of all 1,313 participants, 693 had MAFLD (52.78%). There were a positive association between METS-IR (OR = 1.162, 95% CI = 1.126–1.199) and HOMA-IR (OR = 1.630, 95% CI = 1.431–1.856) and MAFLD risk. The AUCs of the METS-IR and HOMA-IR were 0.831 (0.809, 0.853) and 0.767 (0.741, 0.791), respectively, with significantly different predictive performance (P < 0.001). Adding METS-IR/HOMA-IR to the basic model greatly improved the statistical significance for MAFLD. Five high-risk subgroups were identified for MAFLD. PA mediated about 0.81% and 0.78% (indirect effect/total effect) in the association between METS-IR/HOMA-IR and MAFLD.

Conclusions

MAFLD risk might be predicted by METS-IR/HOMA-IR, among which METS-IR performed better. And PA mediated the association between them. More attention should be paid to the therapeutic effect of lifestyle changes on MAFLD.

Highlights

1. Positive associations were found between METS-IR and HOMA-IR and MAFLD risk.

2. METS-IR has better predictive performance for MAFLD risk than HOMA-IR.

3.Two high-risk subgroups were identified for MAFLD by METS-IR: individuals with METS-IR ≥ 40; Hispanic black individuals with 34 ≤ METS-IR < 40 and aged ≥ 46.

4. In the significant association between METS-IR/HOMA-IR and MAFLD, about 0.81% and 0.78% (indirect effect/total effect), respectively, were mediated by physical activity.

Graphical Abstract
Sex hormones differently regulate lipid metabolism genes in primary human hepatocytes
Authors: Seidemann Lena , Lippold Clara Paula , Rohm Carolin Marie , Eckel Julian Connor , Schicht Gerda , Matz-Soja Madlen , Berg Thomas , Seehofer Daniel , Damm Georg
Published: August 2024

Background

Prevalence of metabolic dysfunction-associated steatotic liver disease (MASLD) is higher in men than in women. Hormonal and genetic causes may account for the sex differences in MASLD. Current human in vitro liver models do not sufficiently take the influence of biological sex and sex hormones into consideration.

Methods

Primary human hepatocytes (PHHs) were isolated from liver specimen of female and male donors and cultured with sex hormones (17β-estradiol, testosterone and progesterone) for up to 72 h. mRNA expression levels of 8 hepatic lipid metabolism genes were analyzed by RT-qPCR. Sex hormones and their metabolites were determined in cell culture supernatants by LC-MS analyses.

Results

A sex-specific expression was observed for LDLR (low density lipoprotein receptor) with higher mRNA levels in male than female PHHs. All three sex hormones were metabolized by PHHs and the effects of hormones on gene expression levels varied depending on hepatocyte sex. Only in female PHHs, 17β-estradiol treatment affected expression levels of PPARA (peroxisome proliferator-activated receptor alpha), LIPC (hepatic lipase) and APOL2 (apolipoprotein L2). Further changes in mRNA levels of female PHHs were observed for ABCA1 (ATP-binding cassette, sub-family A, member 1) after testosterone and for ABCA1, APOA5 (apolipoprotein A-V) and PPARA after progesterone treatment. Only the male PHHs showed changing mRNA levels for LDLR after 17β-estradiol and for APOA5 after testosterone treatment.

Conclusions

Male and female PHHs showed differences in their expression levels of hepatic lipid metabolism genes and their responsiveness towards sex hormones. Thus, cellular sex should be considered, especially when investigating the pathophysiological mechanisms of MASLD.
Developing research priorities for palliative care in Colombia: a priority setting partnership approach
Authors: McConnell Tracey , Mendieta Cindy V. , de Vries Esther , Calvache Jose A. , Prue Gillian , Ahmedzai Sam , Reid Joanne
Published: August 2024

Background

A recent Lancet commission called for more research on palliative care in low- and middle-income (LMIC) countries such as Colombia. A research priority setting approach has been recommended by The Global Forum for Health Research to address the huge gap in research output between LMIC and high-income countries, with influential health service bodies recommending the active involvement of non-research expert stakeholders in establishing research priorities to address service user needs.

Method

Priority setting partnership (PSP) following the four stages of the James Lind Alliance methodology; establishing the partnership, identifying evidence uncertainties, refining questions and uncertainties, and prioritization. Data from MS forms were analysed using descriptive statistics.

Results

A total of 33 stakeholders attended an online PSP workshop and completed the Mentimeter exercise in Microsoft Teams. A total of 48 attended the subsequent in person prioritisation exercise in urban Bogota (n = 22) and rural Popayan (n = 25). The stakeholders were a diverse group of health professionals (physicians, medical students, nurses, dentists, physiotherapists, nutritionist, occupational and speech therapists), financial and administrative staff and patients with life-limiting illness and caregivers. Top research priorities included patient and caregiver needs, service provider education and training, and better integration of palliative care with cancer and non-cancer services. The key challenges included a lack of interest in palliative care research, along with funding, time and resource constraints. Key solutions included collaboration across disciplines and settings, highlighting benefits of palliative research to help secure adequate resources, and multicentre, mixed method research, with patient involvement from the research development stage.

Conclusion

The findings of this PSP should be disseminated among palliative care associations worldwide to inform international multicentre studies, and among governmental and nongovernmental organisations that promote research in Colombia. A focus on patient and family caregiver palliative care needs in Colombia should be prioritised.
The effect of salidroside in promoting endogenous neural regeneration after cerebral ischemia/reperfusion involves notch signaling pathway and neurotrophic factors
Authors: Zheng Jiabing , Zhang Jizhou , Han Jing , Zhao Zhichang , Lin Kan
Published: August 2024

Background

Salidroside is the major bioactive and pharmacological active substance in Rhodiola rosea L. It has been reported to have neuroprotective effects on cerebral ischemia/reperfusion (I/R). However, whether salidroside can enhance neural regeneration after cerebral I/R is still unknown. This study investigated the effects of salidroside on the endogenous neural regeneration after cerebral I/R and the related mechanism.

Methods

Focal cerebral I/R was induced in rats by transient middle cerebral artery occlusion/reperfusion (MCAO/R). The rats were intraperitoneally treated salidroside once daily for 7 consecutive days. Neurobehavioral assessments were performed at 3 days and 7 days after the injury. TTC staining was performed to assess cerebral infarct volume. To evaluate the survival of neurons, immunohistochemical staining of Neuronal Nuclei (NeuN) in the ischemic hemisphere were conducted. Also, immunofluorescence double or triple staining of the biomarkers of proliferating neural progenitor cells in Subventricular Zone (SVZ) and striatum of the ischemia hemisphere were performed to investigate the neurogenesis. Furthermore, reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) were used to detect the expression of neurotrophic factors (NTFs) brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF). Expression of Notch1 and its target molecular Hes1 were also analyzed by western-blotting and RT-PCR.

Results

Salidroside treatment ameliorated I/R induced neurobehavioral impairment, and reduced infarct volume. Salidroside also restored NeuN positive cells loss after I/R injury. Cerebral I/R injury significantly increased the expression of 5-Bromo-2’-Deoxyuridine (BrdU) and doublecotin (DCX), elevated the number of BrdU/Nestin/DCX triple-labeled cells in SVZ, and BrdU/Nestin/glial fibrillary acidic protein (GFAP) triple-labeled cells in striatum. Salidroside treatment further promoted the proliferation of BrdU/DCX labeled neuroblasts and BrdU/Nestin/GFAP labeled reactive astrocytes. Furthermore, salidroside elevated the mRNA expression and protein concentration of BDNF and NGF in ischemia periphery area, as well. Mechanistically, salidroside elevated Notch1/Hes1 mRNA expression in SVZ. The protein levels of them were also increased after salidroside administration.

Conclusions

Salidroside enhances the endogenous neural regeneration after cerebral I/R. The mechanism of the effect may involve the regulation of BDNF/NGF and Notch signaling pathway.
Association of microbial community structure with gill disease in marine-stage farmed Atlantic salmon (Salmo salar); a yearlong study
Authors: Clinton Morag , Wyness Adam J. , Martin Samuel A. M. , Brierley Andrew S. , Ferrier David E. K.
Published: August 2024

Background

Understanding the relationship between resident microbiota and disease in cultured fish represents an important and emerging area of study. Marine gill disorders in particular are considered an important challenge to Atlantic salmon (Salmo salar) aquaculture, however relatively little is known regarding the role resident gill microbiota might play in providing protection from or potentiating different gill diseases. Here, 16S rRNA sequencing was used to examine the gill microbiome alongside fish health screening in farmed Atlantic salmon. Results were used to explore the relationship between microbial communities and gill disease.

Results

Microbial community restructuring was observed throughout the sampling period and linked to varied drivers of change, including environmental conditions and severity of gill pathology. Taxa with significantly greater relative abundance on healthier gills included isolates within genus Shewanella, and taxa within family Procabacteriaceae. In contrast, altered abundance of Candidatus Branchiomonas and Rubritalea spp. were associated with damaged gills. Interestingly, more general changes in community richness and diversity were not associated with altered gill health, and thus not apparently deleterious to fish. Gross and histological gill scoring demonstrated seasonal shifts in gill pathology, with increased severity of gill damage in autumn. Specific infectious causes that contributed to observed pathology within the population included the gill disorder amoebic gill disease (AGD), however due to the uncontrolled nature of this study and likely mixed contribution of various causes of gill disease to observed pathology results do not strongly support an association between the microbial community and specific infectious or non-infectious drivers of gill pathology.

Conclusions

Results suggest that the microbial community of farmed Atlantic salmon gills undergo continual restructuring in the marine environment, with mixed influences upon this change including environmental, host, and pathogenic factors. A significant association of specific taxa with different gill health states suggests these taxa might make meaningful indicators of gill health. Further research with more frequent sampling and deliberate manipulation of gills would provide important advancement of knowledge in this area. Overall, although much is still to be learnt regarding what constitutes a healthy or maladapted gill microbial community, the results of this study provide clear advancement of the field, providing new insight into the microbial community structure of gills during an annual production cycle of marine-stage farmed Atlantic salmon.