bluuebunny/crossref_metadata_2025 huggingface dataset:
Combination of Variable Loads in Structural Design
Authors: Poutanen Tuomo
Published: July 2024

This study delves into the intricacies of variable load combination factors (ψ) within structural codes under fundamental design scenarios, with Eurocodes serving as the primary reference. Currently, variable loads are combined by adding one load, the leading load with its full value, and the other load, the accompanying load, with a reduced value multiplied by a combination factor ψ. This approach employs an independent load combination methodology, utilizing hypothetical reference materials. In contrast, this paper advocates for a shift towards dependent load combination, anchored in the use of actual reference materials. Specifically, it is proposed that imposed loads be combined without the combination factor, i.e., ψ = 1. Given that combination factors are in approximate unity or pertain to infrequent load cases, this research recommends the elimination of ψ from codes altogether. This recommendation stems from the recognition that the current combination factor calculation excels in cases with approximately equal loads with a significant reliability gain, while more frequent unequal loads introduce a minor reliability gain and harmful unsafe errors. Despite the overall minor safety advantage of about 2–3% being negligible considering unavoidable safe errors of about 7% in codes, this simplification significantly reduces code complexity, enhances user-friendliness, and substantially decreases the workload associated with design processes.
An Image-Retrieval Method Based on Cross-Hardware Platform Features
Authors: Yin Jun , Wu Fei , Su Hao
Published: July 2024

Artificial intelligence (AI) models have already achieved great success in fields such as computer vision and natural language processing. However, deploying AI models based on heterogeneous hardware is difficult to ensure accuracy consistency, especially for precision sensitive feature-based image retrieval. In this article, we realize an image-retrieval method based on cross-hardware platform features, aiming to prove that the features of heterogeneous hardware platforms can be mixed, in which the Huawei Atlas 300V and NVIDIA TeslaT4 are used for experiments. First, we compared the decoding differences of heterogeneous hardware, and used CPU software decoding to help hardware decoding improve the decoding success rate. Then, we compared the difference between the Atlas 300V and TeslaT4 chip architectures and tested the differences between the two platform features by calculating feature similarity. In addition, the scaling mode in the pre-processing process was also compared to further analyze the factors affecting feature consistency. Next, the consistency of capture and correlation based on video structure were verified. Finally, the experimental results reveal that the feature results from the TeslaT4 and Atlas 300V can be mixed for image retrieval based on cross-hardware platform features. Consequently, cross-platform image retrieval with low error is realized. Specifically, compared with the Atlas 300V hard and CPU soft decoding, the TeslaT4 hard decoded more than 99% of the image with a decoding pixel maximum difference of +1/−1. From the average of feature similarity, the feature similarity between the Atlas 300V and TeslaT4 exceeds 99%. The difference between the TeslaT4 and Atlas 300V in recall and mAP in feature retrieval is less than 0.1%.
Li-Ion Batteries with a Binder-Free Cathode of Carbon Nanotubes-LiFePO4-Al Foam
Authors: Jin Ying , Wei Shaoxin , Yang Zhoufei , Cui Chaojie , Wang Jin , Li Dongliang , Qian Weizhong
Published: July 2024

With the increasing demand for Li resources worldwide, the easy recycling of Li-ion batteries materials becomes essential. We report a binder-free cathode consisting of carbon nanotubes (CNTs) and LiFePO4 (LFP) nanoparticles embedded in a 3D Al network. The electrode stability depends on the CNT ratio, where 3% CNT-wrapping LFPs provide a stable structure free of detachment from Al foam, as observed on Al foil. The binder-free cathode sheet exhibited excellent performance for high-rate discharge and long-term cycle life. Materials on the cathode can be easily detached with ultrasonic treatment when immersed in organic solvent, which is advantageous for a green and high-efficiency strategy of recycling all valuable materials compared to the binder-used electrode.
PRF Lysates Modulate Chemokine Expression in Oral Squamous Carcinoma and Healthy Epithelial Cells
Authors: Afradi Zohreh , Panahipour Layla , Abbas Zadeh Salman , Gruber Reinhard
Published: July 2024

Platelet-rich fibrin (PRF), originally used to support soft tissue healing, is also considered a therapeutic option for treating oral lichen planus and leukoplakia. The progression from the two premalignant lesions to the aggressive malignant oral squamous cell carcinoma involves an inflammatory process linked to chemokine expression. Thus, there is a rationale for studying how PRF modulates the expression of chemokines in oral squamous carcinoma cells. To this aim, we expose the oral squamous carcinoma cell line HSC2 to IL1β and TNFα either alone or in the presence of lysates obtained from solid PRF membranes. We report here that in HSC2 cells, PRF lysates significantly reduce the forced transcription of chemokines, e.g., CXCL1, CXCL2, CXCL8, CXCL10, and CCL5. Moreover, PRF lysates attenuate the nuclear translocation of p65 in HSC2 oral epithelial cells when exposed to IL1β and TNFα. PRF lysates further reduce chemokine expression provoked by poly:IC HMW. Even though less pronounced, PRF lysates reduce IL1β- and TNFα-induced chemokine expression in TR146 cells. In primary oral epithelial cells, however, PRF lysates increase the basal expression of CXCL1, CXCL2 and CXCL8. Thus, PRF can exert a biphasic effect on chemokine expression in oral squamous cell carcinoma cell lines and primary oral epithelial cells. These findings suggest that PRF may reduce inflammation in a malignant environment while provoking an immunological response in healthy oral epithelium.
Mesenchymal Stem Cell and Hematopoietic Stem and Progenitor Cell Co-Culture in a Bone-Marrow-on-a-Chip Device toward the Generation and Maintenance of the Hematopoietic Niche
Authors: Kefallinou Dionysia , Grigoriou Maria , Boumpas Dimitrios T. , Tserepi Angeliki
Published: July 2024

Bone marrow has raised a great deal of scientific interest, since it is responsible for the vital process of hematopoiesis and is affiliated with many normal and pathological conditions of the human body. In recent years, organs-on-chips (OoCs) have emerged as the epitome of biomimetic systems, combining the advantages of microfluidic technology with cellular biology to surpass conventional 2D/3D cell culture techniques and animal testing. Bone-marrow-on-a-chip (BMoC) devices are usually focused only on the maintenance of the hematopoietic niche; otherwise, they incorporate at least three types of cells for on-chip generation. We, thereby, introduce a BMoC device that aspires to the purely in vitro generation and maintenance of the hematopoietic niche, using solely mesenchymal stem cells (MSCs) and hematopoietic stem and progenitor cells (HSPCs), and relying on the spontaneous formation of the niche without the inclusion of gels or scaffolds. The fabrication process of this poly(dimethylsiloxane) (PDMS)-based device, based on replica molding, is presented, and two membranes, a perforated, in-house-fabricated PDMS membrane and a commercial poly(ethylene terephthalate) (PET) one, were tested and their performances were compared. The device was submerged in a culture dish filled with medium for passive perfusion via diffusion in order to prevent on-chip bubble accumulation. The passively perfused BMoC device, having incorporated a commercial poly(ethylene terephthalate) (PET) membrane, allows for a sustainable MSC and HSPC co-culture and proliferation for three days, a promising indication for the future creation of a hematopoietic bone marrow organoid.
Characterization of Cochlear Implant Artifact and Removal Based on Multi-Channel Wiener Filter in Unilateral Child Patients
Authors: Rossi Dario , Cartocci Giulia , Inguscio Bianca M. S. , Capitolino Giulia , Borghini Gianluca , Di Flumeri Gianluca , Ronca Vincenzo , Giorgi Andrea , Vozzi Alessia , Capotorto Rossella , Babiloni Fabio , Scorpecci Alessandro , Giannantonio Sara , Marsella Pasquale , Leone Carlo Antonio , Grassia Rosa , Galletti Francesco , Ciodaro Francesco , Galletti Cosimo , Aricò Pietro
Published: July 2024

Cochlear implants (CI) allow deaf patients to improve language perception and improving their emotional valence assessment. Electroencephalographic (EEG) measures were employed so far to improve CI programming reliability and to evaluate listening effort in auditory tasks, which are particularly useful in conditions when subjective evaluations are scarcely appliable or reliable. Unfortunately, the presence of CI on the scalp introduces an electrical artifact coupled to EEG signals that masks physiological features recorded by electrodes close to the site of implant. Currently, methods for CI artifact removal have been developed for very specific EEG montages or protocols, while others require many scalp electrodes. In this study, we propose a method based on the Multi-channel Wiener filter (MWF) to overcome those shortcomings. Nine children with unilateral CI and nine age-matched normal hearing children (control) participated in the study. EEG data were acquired on a relatively low number of electrodes (n = 16) during resting condition and during an auditory task. The obtained results obtained allowed to characterize CI artifact on the affected electrode and to significantly reduce, if not remove it through MWF filtering. Moreover, the results indicate, by comparing the two sample populations, that the EEG data loss is minimal in CI users after filtering, and that data maintain EEG physiological characteristics.
FhMYB108 Regulates the Expression of Linalool Synthase Gene in Freesia hybrida and Arabidopsis
Authors: Yang Zhongzhou , Jin Wei , Luo Qi , Li Xiaoli , Wei Yunmin , Lin Yunlong
Published: July 2024

Acting as the most abundant and widely distributed volatile secondary metabolites in plants, terpenoids play crucial roles in diverse physiological regulations and metabolic processes. Terpene synthases play a decisive role in determining the composition and diversity of terpenoids. Though the regulation of terpene synthases has been extensively investigated across various plant species, limited studies have focused on the upstream transcriptional regulation of terpene synthases. In this study, we have identified linalool as the predominant volatile compound that is released gradually from Freesia hybrida flowers throughout flower blooming. In the context of the transcriptome, a typical MYB transcription factor, FhMYB108, was screened based on homologous gene comparison. FhMYB108 is capable of regulating the expression of FhTPS1, and both their expression levels showed gradual increase during flower opening. Moreover, FhMYB108 exerts a stimulatory effect on the transcription of Arabidopsis thaliana AtTPS14, while no significant increase in AtTPS14 expression is observed upon the stabilization of FhMYB108 in A. thaliana. The highly expressed AtMYC2 in A. thaliana could interact with FhMYB108 to suppress the activation of AtTPS14 by FhMYB108. The present study not only elucidates the regulatory mechanism underlying linalool synthesis but also discovers the synergistic effect of MYB and bHLH transcription factors in governing the biosynthesis of volatile terpenoids.
The Impact of Diabetes on Male Silkworm Reproductive Health
Authors: Zeng Xiaoyan , Tong Li
Published: July 2024

The increasing prevalence of diabetic reproductive complications has prompted the development of innovative animal models. The use of the silkworm Bombyx mori as a model for diabetic reproductive damage shows potential as a valuable research tool. This study employed silkworms as a novel model to investigate diabetic reproductive damage. The silkworms were fed a high-glucose diet containing 10% glucose to induce a diabetic model. Subsequently, the study concentrated on assessing the influence of diabetes on the reproductive system of male silkworms. The results indicate that diabetes resulted in reduced luteinizing hormone (LH) and testosterone (T) levels, as well as elevated triglyceride (TG) levels in male silkworms. Moreover, diabetes mellitus was associated with pathological testicular damage in male silkworms, accompanied by decreased glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) levels, along with increased malondialdehyde (MDA) levels in the testis. Additionally, diabetes mellitus reduced the expression of siwi1 and siwi2 genes in the testis of male silkworms. Overall, these results support using silkworms as a valuable model for studying diabetic reproductive damage.
Acetylome Analyses Provide New Insights into the Effect of Chronic Intermittent Hypoxia on Hypothalamus-Dependent Endocrine Metabolism Impairment
Authors: Kong Yaru , Ji Jie , Zhan Xiaojun , Yan Weiheng , Liu Fan , Ye Pengfei , Wang Shan , Tai Jun
Published: July 2024

Paediatric obstructive sleep apnoea (OSA) is a highly prevalent sleep disorder resulting in chronic intermittent hypoxia (CIH) that has been linked to metabolism and endocrine impairment. Protein acetylation, which is a frequently occurring posttranslational modification, plays pivotal roles in the regulation of hypothalamic processes. However, the effects of CIH-induced global protein acetylation on hypothalamic function and endocrine metabolism remain poorly understood. To bridge this knowledge gap, we conducted a study utilizing liquid chromatography–mass spectrometry to analyse the lysine acetylome and proteome of the hypothalamus in healthy infantile mice exposed to 3 weeks of intermittent hypoxia (as a CIH model) compared to normoxic mice (as controls). Our analysis identified and quantified 2699 Kac sites in 2453 proteins. These acetylated proteins exhibited disruptions primarily in endocrine metabolism, the citrate cycle (TCA cycle), synapse function, and circadian entrainment. Additionally, we observed significant down-regulation of proteins that are known to be involved in endocrine hormone secretion. This study aimed to elucidate the molecular mechanisms underlying CIH-induced alterations in protein acetylation within the hypothalamus. By providing valuable insights into the pathophysiological processes associated with CIH and their impacts on hypothalamic function, our findings contribute to a deeper understanding of the consequences stemming from CIH-induced changes in protein acetylation within the hypothalamus as well as its potential role in endocrine impairment.
Complete Plastid Genome Sequences of Four Salsoleae s.l. Species: Comparative and Phylogenetic Analyses
Authors: Almerekova Shyryn , Yermagambetova Moldir , Osmonali Bektemir , Vesselova Polina , Turuspekov Yerlan , Abugalieva Saule
Published: July 2024

The taxonomic classification of the genera Salsola L., Pyankovia Akhani and Roalson, and Xylosalsola Tzvelev within Chenopodiaceae Vent. (Amaranthaceae s.l.) remains controversial, with the precise number of species within these genera still unresolved. This study presents a comparative analysis of the complete plastid genomes of S. foliosa, S. tragus, P. affinis, and X. richteri species collected in Kazakhstan. The assembled plastid genomes varied in length, ranging from 151,177 bp to 152,969 bp for X. richteri and S. tragus. These genomes contained 133 genes, of which 114 were unique, including 80 protein-coding, 30 tRNA, and 4 rRNA genes. Thirteen regions, including ndhC-ndhD, rps16-psbK, petD, rpoC2, ndhA, petB, clpP, atpF, ycf3, accD, ndhF-ndhG, matK, and rpl20-rpl22, exhibited relatively high levels of nucleotide variation. A total of 987 SSRs were detected across the four analyzed plastid genomes, primarily located in the intergenic spacer regions. Additionally, 254 repeats were identified, including 92 tandem repeats, 88 forward repeats, 100 palindromic repeats, and only one reverse repeat. A phylogenetic analysis revealed clear clustering into four clusters corresponding to the Salsoleae and Caroxyloneae tribe clades. These nucleotide sequences obtained in this study represent a valuable resource for future phylogenetic analyses within the Salsoleae s.l. tribe.
Inhibitive Mechanism of Loquat Flower Isolate on Tyrosinase Activity and Melanin Synthesis in Mouse Melanoma B16 Cells
Authors: Chen Qianqian , Tao Wenyang , Wang Jianfeng , Li Jingrui , Zheng Meiyu , Liu Yinying , Lu Shengmin , Fang Zhongxiang
Published: July 2024

Melanin naturally exists in organisms and is synthetized by tyrosinase (TYR); however, its over-production may lead to aberrant pigmentation and skin conditions. Loquat (Eriobotrya japonica (Thunb.) Lindl.) flowers contain a variety of bioactive compounds, while studies on their suppressive capabilities against melanin synthesis are limited. Loquat flower isolate product (LFP) was obtained by ethanol extraction and resin purification, and its inhibitory efficiency against TYR activity was investigated by enzyme kinetics and multiple spectroscopy analyses. In addition, the impact of LFP on melanin synthesis-related proteins’ expression in mouse melanoma B16 cells was analyzed using Western blotting. HPLC-MS/MS analysis indicated that LFP was composed of 137 compounds, of which 12 compounds, including flavonoids (quercetin, isorhamnoin, p-coumaric acid, etc.) and cinnamic acid and its derivatives, as well as benzene and its derivatives, might have TYR inhibitory activities. LFP inhibited TYR activity in a concentration-dependent manner with its IC50 value being 2.8 mg/mL. The inhibition was an anti-competitive one through altering the enzyme’s conformation rather than chelating copper ions at the active center. LFP reduced the expression of TYR, tyrosinase-related protein (TRP) 1, and TRP2 in melanoma B16 cells, hence inhibiting the synthesis of melanin. The research suggested that LFP had the potential to reduce the risks of hyperpigmentation caused by tyrosinase and provided a foundation for the utilization of loquat flower as a natural resource in the development of beauty and aging-related functional products.
Revisiting Fold-Change Calculation: Preference for Median or Geometric Mean over Arithmetic Mean-Based Methods
Authors: Lötsch Jörn , Kringel Dario , Ultsch Alfred
Published: July 2024

Background: Fold change is a common metric in biomedical research for quantifying group differences in omics variables. However, inconsistent calculation methods and inadequate reporting lead to discrepancies in results. This study evaluated various fold-change calculation methods aiming at a recommendation of a preferred approach. Methods: The primary distinction in fold-change calculations lies in defining group expected values for log ratio computation. To challenge method interchangeability in a “stress test” scenario, we generated diverse artificial data sets with varying distributions (identity, uniform, normal, log-normal, and a mixture of these) and compared calculated fold-changes to known values. Additionally, we analyzed a multi-omics biomedical data set to estimate to what extent the findings apply to real-world data. Results: Using arithmetic means as expected values for treatment and reference groups yielded inaccurate fold-change values more frequently than other methods, particularly when subgroup distributions and/or standard deviations differed significantly. Conclusions: The arithmetic mean method, often perceived as standard or picked without considering alternatives, is inferior to other definitions of the group expected value. Methods using median, geometric mean, or paired fold-change combinations are more robust against violations of equal variances or dissimilar group distributions. Adhering to methods less sensitive to data distribution without trade-offs and accurately reporting calculation methods in scientific reports is a reasonable practice to ensure correct interpretation and reproducibility.
Risk Assessment and Personalized Treatment Options in Inherited Dilated Cardiomyopathies: A Narrative Review
Authors: Arnautu Diana-Aurora , Cozma Dragos , Lala Ioan-Radu , Arnautu Sergiu-Florin , Tomescu Mirela-Cleopatra , Andor Minodora
Published: July 2024

Considering the worldwide impact of heart failure, it is crucial to develop approaches that can help us comprehend its root cause and make accurate predictions about its outcome. This is essential for lowering the suffering and death rates connected with this widespread illness. Cardiomyopathies frequently result from genetic factors, and the study of heart failure genetics is advancing quickly. Dilated cardiomyopathy (DCM) is the most prevalent kind of cardiomyopathy, encompassing both genetic and nongenetic abnormalities. It is distinguished by the enlargement of the left ventricle or both ventricles, accompanied by reduced contractility. The discovery of the molecular origins and subsequent awareness of the molecular mechanism is broadening our knowledge of DCM development. Additionally, it emphasizes the complicated nature of DCM and the necessity to formulate several different strategies to address the diverse underlying factors contributing to this disease. Genetic variants that can be transmitted from one generation to another can be a significant contributor to causing family or sporadic hereditary DCM. Genetic variants also play a significant role in determining susceptibility for acquired triggers for DCM. The genetic causes of DCM can have a large range of phenotypic expressions. It is crucial to select patients who are most probable to gain advantages from genetic testing. The purpose of this research is to emphasize the significance of identifying genetic DCM, the relationships between genotype and phenotype, risk assessment, and personalized therapy for both those affected and their relatives. This approach is expected to gain importance once treatment is guided by genotype-specific advice and disease-modifying medications.
The Effect of Sleep Impairment, as Assessed by the CASIS Questionnaire, in Patients with Chronic Obstructive Pulmonary Disease on Disease Severity and Physical and Mental Health: A Cross-Sectional Study in Primary Care
Authors: Bouloukaki Izolde , Christodoulakis Antonios , Margetaki Katerina , Tsiligianni Ioanna
Published: July 2024

The aim of our study was to assess the prevalence of sleep impairment among primary care patients with COPD and explore its impact on disease severity and overall health status. This cross-sectional study included 251 participants > 40 years old from the prospective COCARE COPD study. Data on sociodemographic characteristics, medical history, disease-specific quality of life [COPD Assessment Test (CAT)], COPD severity [Global Initiative for Chronic Obstructive Lung Disease (GOLD) 2023 categorization based on CAT score and exacerbations], fatigue [Fatigue Severity Scale (FSS)], psychological parameters [Patient Health Questionnaire-9 (PHQ-9) and General Anxiety Disorder-7 (GAD-7)], and sleep complaints [COPD and Asthma Sleep Impact Scale (CASIS) and Epworth Sleepiness Scale (ESS)] were collected. Multiple logistic regression analysis was conducted to test for associations of sleep impairment with COPD and overall health status, adjusting for confounders. Sleep impairment, indicated by a CASIS score ≥ 30, increased the risk of worse COPD health status (CAT ≥ 10 OR: 9.7, 95% CI: 5–19, p < 0.001), COPD severity (GOLD B OR: 8.9, 95% CI: 4.5–17.6, p < 0.001 and GOLD E OR: 17.4, 95% CI: 5.1–59.4, p < 0.001), excessive daytime sleepiness (ESS > 10, OR: 3.2, 95% CI: 1.3–8.1, p = 0.012), depressive symptoms (PHQ-9 ≥ 10, OR: 6.4, 95% CI: 2.1–19.1, p = 0.001), anxiety symptoms (GAD-7 ≥ 10, OR: 3.9, 95% CI: 1.6–9.2, p = 0.002), and fatigue (FSS ≥ 36, OR: 5.3, 95% CI: 2.8–9.8, p < 0.001). In conclusion, our findings suggest that sleep impairment, based on the CASIS questionnaire, is associated with worse physical and mental health in patients with COPD. Therefore, through consistent evaluation of sleep and targeted management strategies, healthcare providers could improve the quality of life for these patients.
Sex Differences in Cardiovascular Diseases: Exploring the Role of Microbiota and Immunity
Authors: Franza Laura , Caldarelli Mario , Villani Emanuele Rocco , Cianci Rossella
Published: July 2024

Cardiovascular diseases (CVDs) are the most common cause of mortality and morbidity in Western countries, thus representing a global health concern. CVDs show different patterns in terms of the prevalence and presentation in men and women. The role of sex hormones has been extensively implicated in these sex-specific differences, due to the presence of the menstrual cycle and menopause in women. Moreover, the gut microbiota (GM) has been implicated in cardiovascular health, considering the growing evidence that it is involved in determining the development of specific diseases. In particular, gut-derived metabolites have been linked to CVDs and kidney disorders, which can in turn promote the progression of CVDs. Considering the differences in the composition of GM between men and women, it is possible that gut microbiota act as a mediator in regard to the sex disparities in CVDs. This narrative review aims to comprehensively review the interplay between sex, GM, and CVDs, discussing potential mechanisms and therapeutic options.
Investigation of the Protective Effects of Magnesium on Bupivacaine-Induced Toxicity at the Level of Colon Cell Culture
Authors: Önal Ceren , Saraçoğlu Kemal Tolga , Saraçoğlu Ayten , Özkan Beyza Nur , Güler Eray Metin , Arslan Gülten , Karakuş Seçil Azime , Bulun Yekbun , Gaszynski Tomasz , Ratajczyk Pawel
Published: July 2024

The primary objective of this in vitro study was to prevent the risk of toxicity associated with bupivacaine, widely used in clinical practice, by using magnesium (Mg), a readily available and cost-effective element, as an adjuvant. We hypothesized that Mg might exhibit a protective effect against cytotoxicity in a colon cell culture model under conditions of bupivacaine-induced LAST. Our secondary aim was to investigate its effect on genotoxicity, apoptosis, and iROS. CCD-18Co cells were used in our study. Control group (group C), Bupivacaine group (group B), Magnesium group (group M), and Bupivacaine+Mg group (group BM) were created. The viability of CCD-18Co cells incubated for 24 h in group C was determined to be 100%. These cells were evenly divided, and bupivacaine was administered to group B at concentrations of 5 to 300 μM. In group M, doses of Mg at 0.625 to 320 mEq were added. It was determined that the maximum viability was observed at a Mg dose of 40 mEq (p < 0.05). In group BM, bupivacaine was administered at the same concentrations in combination with Mg (40 mEq), and cell viability was measured. DNA damage, apoptosis, and iROS were assessed at concentrations of bupivacaine by administering 40 mEq Mg. In group B, viability decreased dose-dependently in CCD-18Co (p < 0.05, p < 0.01, p < 0.001). In group BM, the viability decreased in cells at increasing concentrations compared to group C (p < 0.05, p < 0.01, p < 0.001), but the viability was affected positively compared to group B (p < 0.05). In group B, DNA damage increased (p < 0.05, p < 0.001). In group BM, DNA damage increased (p < 0.05, p < 0.001). However, in group BM, DNA damage levels were reduced compared to group B (p < 0.05, p < 0.01). In group B, apoptosis increased (p < 0.05, p < 0.001); in group BM, apoptosis increased (p < 0.001) compared to group C. However, in group BM, apoptosis decreased compared to group B (p< 0.05). iROS increased in group B (p < 0.05, p < 0.01, p < 0.01) and group BM (p < 0.05, p < 0.01, p < 0.001) compared to the group C. However, in group BM, iROS decreased in comparison to group B (p < 0.05). In conclusion, Mg exhibits a protective effect against bupivacaine-induced toxicity.
Peristaltic Motion Enabled by Pneumatic Artificial Muscles (PAMs) as Structural “Soft–Stiff” Actuators in a Modular Worm-Inspired Robot
Authors: Tinsley Beth , Caponi Sergio , McAteer Lucy , Nebesnyy Gleb , Sammanthan Dean , Keza Ella Sonia , Alam Parvez
Published: July 2024

This paper considers the design, manufacture, and testing of a prototype “soft–stiff” worm-inspired robot referred to herein, as the PneumaticallyActuated PeristaLtic Advancing Modular (PALAM) robot. The robot has a modular structure, mimicking the segmented nature of earthworms, and each segment is individually actuated by a set of three pneumatic artificial muscles (PAMs). The PAMs contract when inflated by pressurised air, generating a pulling force and fulfilling the role of biological muscles in the robot. The PAMs are made from the elastomer silicone rubber, which affords the robot flexibility and enables a wide range of real-life applications. A control-system is designed which can inflate any PAM on demand, and hence replicate the peristaltic motion of earthworms in the PALAM robot. Finally, this paper discusses a successful, low-cost, and widely accessible approach for the manufacture of the PAMs utilised herein. The PAMs can be scaled dimensionally and made from different materials with varying mechanical properties and behaviours, meaning that they are suitable for use in a wide range of robotics applications.
A Survey on Biomimetic and Intelligent Algorithms with Applications
Authors: Li Hao , Liao Bolin , Li Jianfeng , Li Shuai
Published: July 2024

The question “How does it work” has motivated many scientists. Through the study of natural phenomena and behaviors, many intelligence algorithms have been proposed to solve various optimization problems. This paper aims to offer an informative guide for researchers who are interested in tackling optimization problems with intelligence algorithms. First, a special neural network was comprehensively discussed, and it was called a zeroing neural network (ZNN). It is especially intended for solving time-varying optimization problems, including origin, basic principles, operation mechanism, model variants, and applications. This paper presents a new classification method based on the performance index of ZNNs. Then, two classic bio-inspired algorithms, a genetic algorithm and a particle swarm algorithm, are outlined as representatives, including their origin, design process, basic principles, and applications. Finally, to emphasize the applicability of intelligence algorithms, three practical domains are introduced, including gene feature extraction, intelligence communication, and the image process.
Adjuvant Therapy with Immune Checkpoint Inhibitors after Carbon Ion Radiotherapy for Mucosal Melanoma of the Head and Neck: A Case-Control Study
Authors: Mizoguchi Nobutaka , Kano Kio , Okuda Tatsuya , Koge Hiroaki , Shima Satoshi , Tsuchida Keisuke , Takakusagi Yosuke , Kawashiro Shohei , Yoshida Manatsu , Kitani Yuka , Hashimoto Kaori , Furukawa Madoka , Shirai Katsuyuki , Kamada Tadashi , Yoshida Daisaku , Katoh Hiroyuki
Published: July 2024

The development of new treatment strategies to improve the prognosis of mucosal malignant melanoma of the head and neck (MMHN) after carbon ion radiotherapy (CIRT) is essential because of the risk of distant metastases. Therefore, our objective was to evaluate the outcomes of immune checkpoint inhibitor (ICI) treatment to justify its inclusion in the regimen after CIRT. Thirty-four patients who received CIRT as an initial treatment were included in the analysis and stratified into three groups: those who did not receive ICIs (Group A), those who received ICIs after recurrence or metastasis (Group B), and those who received ICIs as adjuvant therapy after CIRT (Group C). In total, 62% of the patients (n = 21) received ICIs. The 2-year local control and overall survival (OS) rates for all patients were 90.0% and 66.8%, respectively. The 2-year OS rates for patients in Groups A, B, and C were 50.8%, 66.7%, and 100%, respectively. No significant differences were observed between Groups A and B (p = 0.192) and Groups B and C (p = 0.112). However, a significant difference was confirmed between Groups A and C (p = 0.017). Adjuvant therapy following CIRT for MMHN may be a promising treatment modality that can extend patient survival.
Detecting Atypical Sentinel Lymph Nodes in Early-Stage Cervical Cancer Using a Standardized Technique with a Hybrid Tracer
Authors: Amengual Vila Joana , Torrent Colomer Anna , Sampol Bas Catalina , Quintero Duarte Adriana , Ruiz Coll Mario , Rioja Merlo Jorge , Cordoba Octavi
Published: July 2024

Background: Since October 2018, lymph node status has become part of the FIGO staging, given that it is one of the most important prognostic factors among women with CC. The aim was to determine the rate of atypical lymphatic drainage in patients with clinical early-stage cervical cancer using a hybrid tracer (ICG-99mTc nanocolloid). Methodology: A prospective, observational, single-centre study conducted at Son Espases University Hospital between January 2019 and October 2023. Patients with clinical early-stage CC who underwent SLN mapping were included. External iliac and obturator nodes were defined as common locations. Para-aortic, common iliac, presacral, internal iliac, and parametrial nodes were defined as atypical locations. Results: Thirty-nine cases of CC were included. The overall SLN detection rate was 97.4%, with 89.5% bilaterally. Positive nodes were found in 21.1% of patients. Atypical lymphatic drainage was present in 8 out of 38 (21.1%) patients. Of all the SLNs biopsied (146), 10.3% corresponded to an atypical zone. SLN in the atypical area had a higher proportion of metastasis than the usual area (37.5% vs. 16.7%; p = 0.327). Conclusions: SLN biopsy can detect unusual drainage in a significant proportion of patients. Atypical lymph nodes have a higher percentage of metastasis, which consequently improves staging and tailoring therapy. SLN mapping performed via a standardized surgical technique using a hybrid tracer (ICG-99mTc) could help in the identification of the “true SLN”.
Decoding Clonal Hematopoiesis: Emerging Themes and Novel Mechanistic Insights
Authors: Pendse Shalmali , Loeffler Dirk
Published: July 2024

Clonal hematopoiesis (CH), the relative expansion of mutant clones, is derived from hematopoietic stem cells (HSCs) with acquired somatic or cytogenetic alterations that improve cellular fitness. Individuals with CH have a higher risk for hematological and non-hematological diseases, such as cardiovascular disease, and have an overall higher mortality rate. Originally thought to be restricted to a small fraction of elderly people, recent advances in single-cell sequencing and bioinformatics have revealed that CH with multiple expanded mutant clones is universal in the elderly population. Just a few years ago, phylogenetic reconstruction across the human lifespan and novel sensitive sequencing techniques showed that CH can start earlier in life, decades before it was thought possible. These studies also suggest that environmental factors acting through aberrant inflammation might be a common theme promoting clonal expansion and disease progression. However, numerous aspects of this phenomenon remain to be elucidated and the precise mechanisms, context-specific drivers, and pathways of clonal expansion remain to be established. Here, we review our current understanding of the cellular mechanisms driving CH and specifically focus on how pro-inflammatory factors affect normal and mutant HSC fates to promote clonal selection.
Prevalence of Hypertension and Its Associated Factors among Adolescents in Eastern Sudan: A Community-Based Study
Authors: Omar Saeed M. , Hassan Ahmed A. , Al-Nafeesah Abdullah , AlEed Ashwaq , Alfaifi Jaber , Adam Ishag
Published: July 2024

Background: Elevated blood pressure, or hypertension, is one of the main health problems among adolescents globally. However, there are limited data on hypertension among adolescents in Sudan. This survey aimed to investigate the prevalence of elevated blood pressure/hypertension and associated factors among adolescents in Gadarif City, Sudan. Methods: A community-based cross-sectional survey was conducted during a three-month period (August to October 2023) in Gadarif City, Eastern Sudan. A face-to-face interview questionnaire was used to collect sociodemographic information. Adolescents’ anthropometric (weight and height) measurements were taken, and blood pressure was measured. Multivariate binary and linear regression analyses were performed to analyze the data. Results: A total of 384 adolescents (178 [46.4%] boys and 206 [53.6%] girls) were included in the study. The median (interquartile range, IQR) of the age was 14.0 (12.1–16.1) years, and that of the body mass index (BMI) was 16.9 (15.2–20.0) kg/m2. Thirty-four (8.9%) adolescents had hypertension/elevated blood pressure (≥95th percentile). After adjusting for confounders, multivariable binary regression analysis showed that age (adjusted odds ratio [AOR], 1.20; 95% confidence interval [CI], 1.03–1.42) and BMI (AOR, 1.12; 95% CI, 1.04–1.20) were associated with hypertension. Conclusion: Approximately one in ten adolescents in Eastern Sudan was hypertensive. Adolescents with higher age and BMI were at higher risk for hypertension. Maintaining a healthy BMI during adolescence is recommended to promote adolescents’ health.
Relationship between Maternal Stress and Neurobehavioral Indicators of Preterm Infants in the Neonatal Intensive Care Unit
Authors: Ramos Bruna Abreu , Formiga Cibelle Kayenne Martins Roberto , Oliveira Nayara Rodrigues Gomes de , Marçal Patricia Gonçalves Evangelista , Ferreira Rui Gilberto , Saidah Tárik Kassem , Amaral Waldemar Naves do
Published: July 2024

Background: Preterm birth and prolonged neonatal hospitalization are potential sources of stress for mothers of preterm and low birth weight infants. Aim: To evaluate maternal stress and its association with neurobehavioral indicators of preterm infants during hospitalization in the neonatal intensive care unit. Methods: A cross-sectional study was conducted in a neonatal intensive care unit of a hospital in Goiânia, Brazil. The study included preterm and low birth weight infants of both genders and their mothers. The Parental Stressor Scale: Neonatal Intensive Care Unit and the Neurobehavioral Assessment of the Preterm Infant were respectively applied to mothers and infants in the neonatal intensive care unit. Results: The study involved 165 premature infants and their mothers. The mean age of the mothers was 26.3 years and most had a high school education level (57.6%). Mothers perceived the experience of having an infant in the neonatal intensive care unit as moderately stressful (2.96 ± 0.81). The parental role alteration (4.11 ± 1.03) and sights and sounds (2.15 ± 0.90) subscales exhibited the highest and lowest stress levels, respectively. Significant correlations (rho < −0.3; p < 0.05) were found between maternal stress and neurobehavioral indicators of infants. In the multivariate analysis, low leg tone was a predictor of higher maternal stress. Low tone and limited arm movement were predictors of higher maternal stress in the maternal role item. Conclusions: The experience of having a preterm infant hospitalized was considered moderately stressful for mothers. Maternal stress levels were significantly correlated with low scores on neonatal neurobehavioral indicators.
SaBi3d—A LiDAR Point Cloud Data Set of Car-to-Bicycle Overtaking Maneuvers
Authors: Odenwald Christian , Beeking Moritz
Published: July 2024

While cycling presents environmental benefits and promotes a healthy lifestyle, the risks associated with overtaking maneuvers by motorized vehicles represent a significant barrier for many potential cyclists. A large-scale analysis of overtaking maneuvers could inform traffic researchers and city planners how to reduce these risks by better understanding these maneuvers. Drawing from the fields of sensor-based cycling research and from LiDAR-based traffic data sets, this paper provides a step towards addressing these safety concerns by introducing the Salzburg Bicycle 3d (SaBi3d) data set, which consists of LiDAR point clouds capturing car-to-bicycle overtaking maneuvers. The data set, collected using a LiDAR-equipped bicycle, facilitates the detailed analysis of a large quantity of overtaking maneuvers without the need for manual annotation through enabling automatic labeling by a neural network. Additionally, a benchmark result for 3D object detection using a competitive neural network is provided as a baseline for future research. The SaBi3d data set is structured identically to the nuScenes data set, and therefore offers compatibility with numerous existing object detection systems. This work provides valuable resources for future researchers to better understand cycling infrastructure and mitigate risks, thus promoting cycling as a viable mode of transportation.
Data Descriptor of Snakebites in Brazil from 2007 to 2020
Authors: Silva-Neto Alexandre Vilhena , Mouta Gabriel Santos , Silva Balieiro Antônio Alcirley , Cordeiro Jady Shayenne Mota , Balieiro Patricia Carvalho Silva , Ramos Tatyana Costa Amorin , Baia-da-Silva Djane Clarys , Rocha Élisson Silva , Endo Patricia Takako , Lynn Theo , Monteiro Wuelton Marcelo , Sampaio Vanderson Souza
Published: July 2024

Snakebite envenomations (SBE) are a significant global public health threat due to their morbidity and mortality. This is a neglected public health issue in many tropical and subtropical countries. Brazil is in the top ten countries affected by SBE, with 32,160 cases reported only in 2020, posing a high burden for this population. In this paper, we describe the data structure of snakebite records from 2007 to 2020 in the Notifiable Disease Information System (SINAN), made available by the Brazilian Ministry of Health (MoH). In addition, we also provide R scripts that allow a quick and automatic updating of data from the SINAN according to its availability. The data presented in this work are related to clinical and demographic information on SBE cases. Also, data on outcomes, laboratory results, and treatment are available. The dataset is available and freely accessible; however, preprocessing, adjustments, and standardization are necessary due to incompleteness and inconsistencies. Regardless of these limitations, it provides a solid basis for assessing different aspects and the national burden of envenoming.
Optimizing Database Performance in Complex Event Processing through Indexing Strategies
Authors: Abbasi Maryam , Bernardo Marco V. , Váz Paulo , Silva José , Martins Pedro
Published: July 2024

Complex event processing (CEP) systems have gained significant importance in various domains, such as finance, logistics, and security, where the real-time analysis of event streams is crucial. However, as the volume and complexity of event data continue to grow, optimizing the performance of CEP systems becomes a critical challenge. This paper investigates the impact of indexing strategies on the performance of databases handling complex event processing. We propose a novel indexing technique, called Hierarchical Temporal Indexing (HTI), specifically designed for the efficient processing of complex event queries. HTI leverages the temporal nature of event data and employs a multi-level indexing approach to optimize query execution. By combining temporal indexing with spatial- and attribute-based indexing, HTI aims to accelerate the retrieval and processing of relevant events, thereby improving overall query performance. In this study, we evaluate the effectiveness of HTI by implementing complex event queries on various CEP systems with different indexing strategies. We conduct a comprehensive performance analysis, measuring the query execution times and resource utilization (CPU, memory, etc.), and analyzing the execution plans and query optimization techniques employed by each system. Our experimental results demonstrate that the proposed HTI indexing strategy outperforms traditional indexing approaches, particularly for complex event queries involving temporal constraints and multi-dimensional event attributes. We provide insights into the strengths and weaknesses of each indexing strategy, identifying the factors that influence performance, such as data volume, query complexity, and event characteristics. Furthermore, we discuss the implications of our findings for the design and optimization of CEP systems, offering recommendations for indexing strategy selection based on the specific requirements and workload characteristics. Finally, we outline the potential limitations of our study and suggest future research directions in this domain.
Comparison of Aesthetic, Mechanical Outcome, and Bone Loss in Angulated Screw Channels (ASCs) and Cement-Retained Implant-Supported Prosthesis: A Case-Control Study
Authors: Rella Edoardo , De Angelis Paolo , Papetti Laura , Damis Giovanni , Gasparini Giulio , D’Addona Antonio , Manicone Paolo Francesco
Published: July 2024

Angulated-screw channels (ASCs) allow the clinician to employ screw-retained restorations in almost all cases, as the access hole can be moved away from the vestibular portion of the crown, where it would jeopardize the final esthetic result. The objective of this study was to compare screw-retained restorations employing ASCs with restorations cemented on angled abutments. In this study, 30 subjects, equally divided into two groups: group 1 (cemented restorations on angulated abutments) and group 2 (screw-retained restorations adopting ASCs), were treated and retrospectively compared after 2 years using the pink esthetic score (PES) and the white esthetic score (WES). All restorations were in use at the last follow-up, with a survival rate of 100%. Three mechanical complications were observed (2 chippings and 1 crown came loose), with a success rate of 93% in group 1 and 87% in group 2 (p > 0.05). No statistically significant differences were reported regarding the esthetic outcome; the marginal bone loss (MBL) showed better results for the screw-retained restorations, both at the distal aspect (group 1 = 0.98 mm ± 0.16; group 2 = 0.45 mm ± 0.06; p = 0.006) and at the mesial aspect (group 1 = 1.04 ± 0.27; group 2 = 0.45 ± 0.005; p < 0.001). From an esthetical perspective, screw-retained restorations with ASCs and cemented restorations on angulated abutments are both effective means of restoring implants; both have excellent esthetic outcomes, but screw-retained restorations have reduced bone loss when compared to cemented ones but are more prone to mechanical complications. Still, our results must be cautiously observed given the reduced dimension of our sample. Larger studies are needed to confirm our findings.