bluuebunny/crossref_metadata_2025 huggingface dataset:
Association between Sudden Sensorineural Hearing Loss and Preexisting Thyroid Diseases: A Nationwide Case-Control Study in Taiwan
Authors: Tsai Yao-Te , Chang I-Jen , Hsu Cheng-Ming , Yang Yao-Hsu , Liu Chia-Yen , Tsai Ming-Shao , Chang Geng-He , Lee Yi-Chan , Huang Ethan I. , Lin Meng-Hung , Luan Chih-Wei
Published: January 2020

Background: Little evidence is available about the risk of sudden sensorineural hearing loss (SSNHL) in patients with thyroid diseases. We assessed whether a diagnosis of thyroid disease, particularly hyperthyroidism or hypothyroidism, is associated with SSNHL risk in an Asian population. Material and Methods: This case-control study was conducted with population-based data from Taiwan’s National Health Insurance Research Database from January 2000 to December 2013. The case group comprised 3331 adult patients with newly diagnosed SSNHL, and four controls without SSNHL for each case matched by sex, age, monthly income, and urbanization level of residence. Underlying Thyroid diseases were retrospectively evaluated in the case and control groups. Multivariate logistic regression analyses were used to explore relations between thyroid diseases and SSNHL. Results: Of the 3331 cases, 5.7% had preexisting thyroid diseases, whereas only 4.0% of the 13,324 controls had the same condition. After adjustment for sex, age, monthly income, urbanization level of residence, history of hypertension, diabetes mellitus, chronic otitis media, and hyperlipidemia, associations were identified between a history of either hypothyroidism (adjusted odds ratio [AOR], 1.54; 95% CI, 1.02–2.32; p = 0.042) or hyperthyroidism (AOR, 1.41; 95% CI, 1.07–1.85; p = 0.015) and an elevated risk of SSNHL. In subgroup analysis, the correlation between hypothyroidism and increased SSNHL risk remained significant only for patients aged over 50 years (AOR, 1.61; 95% CI, 1.01–2.57; p = 0.045), and that between hyperthyroidism and SSNHL was significant only for female patients (AOR, 1.48; 95% CI, 1.09–2.01; p = 0.012). Treatment for hypothyroidism and hyperthyroidism did not alter the association in subgroup analyses. Conclusion: Preexisting hypothyroidism and hyperthyroidism appear associated with SSNHL susceptibility in Taiwan. Physicians should be wary of this elevated risk of SSNHL among patients with previously diagnosed thyroid dysfunction, especially women and patients aged more than 50 years.
Impact of Nurse-Patient Relationship on Quality of Care and Patient Autonomy in Decision-Making
Authors: Molina-Mula Jesús , Gallo-Estrada Julia
Published: January 2020

Background: The patient is observed to acquire a passive role and the nurse an expert role with a maternalistic attitude. This relationship among others determines the capacity for autonomy in the decision making of patients. Objectives: The aim of this study is to analyse the nurse-patient relationship and explore their implications for clinical practice, the impact on quality of care, and the decision-making capacity of patients. Design: A phenomenological qualitative study was conducted. Settings and participants: Thirteen in-depth interviews with nurses and 61,484 nursing records from internal medicine and specialties departments in a general hospital from 2015–2016. Methods: A discourse analysis and triangulation for these sources were conducted. Results: The category elaborated from nursing records was defined according to the following codes: Good Patient, Bad patient, and Social Problem. Analysis of the interviews resulted in a category defined as Patient as a passive object. Discussion: A good nurse-patient relationship reduces the days of hospital stay and improves the quality and satisfaction of both. However, in contrast, the good relationship is conditioned by the patient’s submissive role. Conclusion: An equal distribution of power allows decisions about health and disease processes to be acquired by patients, autonomously, with the advice of professionals. The nurse-patient relationship should not pursue the change in values and customs of the patient, but position the professional as a witness of the experience of the health and illness process in the patient and family.
Keeping People with Dementia or Mild Cognitive Impairment in Employment: A Literature Review on Its Determinants
Authors: Silvaggi Fabiola , Leonardi Matilde , Tiraboschi Pietro , Muscio Cristina , Toppo Claudia , Raggi Alberto
Published: January 2020

Background: Approximately 10–20% of people with early onset dementias (EOD) or mild cognitive impairment (MCI) are aged under 65 and, due to extended working life and increasing prevalence of dementias, they more and more frequently will be present in the active workforce. This review aimed to synthesize the available information about the ability of people with EOD or MCI to retain their participation in the labor workforce. Methods: We searched SCOPUS and EMBASE for peer-reviewed papers that reported studies assessing work ability in employees with EOD or MCI that were published in the period of January 2010 to August 2019. Results: We selected four publications, in which 1012 participants with EOD or MCI were enrolled (41.2% males). Cognitive difficulties rather than motor dysfunction were found to reduce patients’ ability to work. Two main themes emerged: management of dementia in the workplace and the impact of symptoms on working status. Conclusions: EOD and MCI impact on workforce participation by determining problems in executive functions. Although this review was based on a small sample of studies, it can be shown that support in the workplace may act as a facilitator to enhance workforce participation, and occupational health professionals can help patients with EOD or MCI continue working as much as possible.
Problematic Smartphone Use and Mental Health in Chinese Adults: A Population-Based Study
Authors: Guo Ningyuan , Luk Tzu Tsun , Ho Sai Yin , Lee Jung Jae , Shen Chen , Oliffe John , Chan Sophia Siu-Chee , Lam Tai Hing , Wang Man Ping
Published: January 2020

Problematic smartphone use (PSU) has been associated with anxiety and depression, but few explored its mental well-being correlates that could co-occur with or be independent of mental symptoms. We studied the associations of PSU with anxiety, depression, and mental well-being in Hong Kong Chinese adults in a probability-based survey (N = 4054; 55.0% females; mean age ± SD 48.3 ± 18.3 years). PSU was measured using Smartphone Addiction Scale-Short Version. Anxiety and depression symptoms were evaluated using General Anxiety Disorder screener-2 (GAD-2) and Patient Health Questionnaire-2 (PHQ-2). Mental well-being was measured using Subjective Happiness Scale (SHS) and Short Warwick-Edinburgh Mental Well-Being Scale (SWEMWBS). Multivariable regression analyzed associations adjusting for sociodemographic and lifestyle-related variables. Associations of PSU with mental well-being were stratified by symptom severity of anxiety (GAD-2 cutoff of 3) and depression (PHQ-2 cutoff of 3). We found that PSU was associated with higher odds of anxiety and depression symptom severity and lower scores of SHS and SWEMWBS. Associations of PSU with lower SHS and SWEMWBS scores remained in respondents who screened negative for anxiety or depression symptoms. To conclude, PSU was associated with anxiety, depression, and impaired mental well-being. Associations of PSU with impaired mental well-being could be independent of anxiety or depression symptoms.
Effectiveness and Durability of Transfer Training in Fencing
Authors: Witkowski Mateusz , Bojkowski Łukasz , Karpowicz Krzysztof , Konieczny Mariusz , Bronikowski Michał , Tomczak Maciej
Published: January 2020

This paper reports the results of an experiment that aimed to study transfer training in fencing. Fencers from the experimental group underwent six-week transfer training while those from the control group underwent regular fencing training. The fencers’ performance was analyzed thrice: before the experimental training (pretest), immediately after it (posttest), and four weeks after it (retention test). Using a device that simulates fencing moves and analyzes the accuracy of such performance, participants completed, with both hands, three tests related to straight thrust accuracy. While no differences in hand grip strength was observed between the two groups across the three tests, significant differences occurred in terms of their performance on the device. The groups did not differ in the pretests and the retention tests. However, the fencers from the experimental group generally performed better in postests than prestests. These results show that bilateral transfer can be effective in foil fencing training, although its positive effects are short-term. In order to be effective, transfer training should be used as a regular training tool.
An Application of Item Response Theory to Scoring Patient Safety Culture Survey Data
Authors: Jeong Heon-Jae , Liao Hsun-Hsiang , Han Su Ha , Lee Wui-Chiang
Published: January 2020

Patient safety culture is important in preventing medical errors. Thus, many instruments have been developed to measure it. Yet, few studies focus on the data processing step. This study, by analyzing the Chinese version of the Safety Attitudes Questionnaire dataset that contained 37,163 questionnaires collected in Taiwan, found critical issues related to the currently used mean scoring method: The instrument, like other popular ones, uses a 5-point Likert scale, and because it is an ordinal scale, the mean scores cannot be calculated. Instead, Item Response Theory (IRT) was applied. The construct validity was satisfactory and the item properties of the instrument were estimated from confirmatory factor analysis. The IRT-based domain scores and mean domain scores of each respondent were estimated and compared. As for resolution, the mean approach yielded only around 20 unique values on a 0 to 100 scale for each domain; the IRT method yielded at least 440 unique values. Meanwhile, IRT scores ranged widely at each unique mean score, meaning that the precision of the mean approach was less reliable. The theoretical soundness and empirical strength of IRT suggest that healthcare institutions should adopt IRT as a new scoring method, which is the core step of processing collected data.
Spectrum of Tendon Pathologies: Triggers, Trails and End-State
Authors: Steinmann Sara , Pfeifer Christian G. , Brochhausen Christoph , Docheva Denitsa
Published: January 2020

The biggest compartment of the musculoskeletal system is the tendons and ligaments. In particular, tendons are dense tissues connecting muscle to bone that are critical for the integrity, function and locomotion of this system. Due to the increasing age of our society and the overall rise in engagement in extreme and overuse sports, there is a growing prevalence of tendinopathies. Despite the recent advances in tendon research and due to difficult early diagnosis, a multitude of risk factors and vague understanding of the underlying biological mechanisms involved in the progression of tendon injuries, the toolbox of treatment strategies remains limited and non-satisfactory. This review is designed to summarize the current knowledge of triggers, trails and end state of tendinopathies.
PERK-Mediated eIF2α Phosphorylation Contributes to The Protection of Dopaminergic Neurons from Chronic Heat Stress in Drosophila
Authors: Elvira Rosalie , Cha Sun Joo , Noh Gyeong-Mu , Kim Kiyoung , Han Jaeseok
Published: January 2020

Environmental high-temperature heat exposure is linked to physiological stress such as disturbed protein homeostasis caused by endoplasmic reticulum (ER) stress. Abnormal proteostasis in neuronal cells is a common pathological factor of Parkinson’s disease (PD). Chronic heat stress is thought to induce neuronal cell death during the onset and progression of PD, but the exact role and mechanism of ER stress and the activation of the unfolded protein response (UPR) remains unclear. Here, we showed that chronic heat exposure induces ER stress mediated by the PKR-like eukaryotic initiation factor 2α kinase (PERK)/eIF2α phosphorylation signaling pathway in Drosophila neurons. Chronic heat-induced eIF2α phosphorylation was regulated by PERK activation and required for neuroprotection from chronic heat stress. Moreover, the attenuated protein synthesis by eIF2α phosphorylation was a critical factor for neuronal cell survival during chronic heat stress. We further showed that genetic downregulation of PERK, specifically in dopaminergic (DA) neurons, impaired motor activity and led to DA neuron loss. Therefore, our findings provide in vivo evidence demonstrating that chronic heat exposure may be a critical risk factor in the onset of PD, and eIF2α phosphorylation mediated by PERK may contribute to the protection of DA neurons against chronic heat stress in Drosophila.
Transcriptomic Analyses of Camellia oleifera ‘Huaxin’ Leaf Reveal Candidate Genes Related to Long-Term Cold Stress
Authors: Wu Lingli , Li Jian’an , Li Ze , Zhang Fanhang , Tan Xiaofeng
Published: January 2020

‘Huaxin’ is a new high-yielding timber cultivar of Camellia oleifera of high economic value, and has been widely cultivated in the red soil hilly region of Hunan Province of the People´s Republic of China in recent years. However, its quality and production are severely affected by low temperatures during flowering. To find genes related to cold tolerance and further explore new candidategenes for chilling-tolerance, Illumina NGS (Next Generation Sequencing) technology was used to perform transcriptomic analyses of C. oleifera ‘Huaxin’ leaves under long-term cold stress. Nine cDNA libraries were sequenced, and 58.31 Gb high-quality clean reads were obtained with an average of 5.92 Gb reads for each sample. A total of 191,150 transcripts were obtained after assembly. Among them, 100,703 unigenes were generated, and 44,610 unigenes were annotated. In total, 1564 differentially expressed genes (DEGs) were identified both in the A_B and A_C gene sets. In the current study, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed, andrevealed a group of cold-responsive genes related to hormone regulation, photosynthesis, membrane systems, and osmoregulation; these genes encoded many key proteins in plant biological processes, such as serine/threonine-protein kinase (STPK), transcription factors (TFs), fatty acid desaturase (FAD), lipid-transfer proteins (LTPs), soluble sugars synthetases, and flavonoid biosynthetic enzymes. Some physiological indicators of C. oleifera ‘Huaxin’ were determined under three temperature conditions, and the results were consistent with the molecular sequencing. In addition, the expression levels of 12 DEGs were verified using quantitative real-time polymerase chain reaction (qRT-PCR). In summary, the results of DEGs analysis together with qRT-PCR tests contribute to the understanding of cold tolerance and further exploring new candidate genes for chilling-tolerance in molecular breeding programs of C. oleifera ‘Huaxin’.
Editorial of Special Issue “Adipokines 2.0”
Authors: Buechler Christa
Published: January 2020

This editorial aims to summarize the 19 scientific papers that contributed to the Special Issue “Adipokines 2”.
Neutrophil Adaptations upon Recruitment to the Lung: New Concepts and Implications for Homeostasis and Disease
Authors: Giacalone Vincent D. , Margaroli Camilla , Mall Marcus A. , Tirouvanziam Rabindra
Published: January 2020

Neutrophils have a prominent role in all human immune responses against any type of pathogen or stimulus. The lungs are a major neutrophil reservoir and neutrophilic inflammation is a primary response to both infectious and non-infectious challenges. While neutrophils are well known for their essential role in clearance of bacteria, they are also equipped with specific mechanisms to counter viruses and fungi. When these defense mechanisms become aberrantly activated in the absence of infection, this commonly results in debilitating chronic lung inflammation. Clearance of bacteria by phagocytosis is the hallmark role of neutrophils and has been studied extensively. New studies on neutrophil biology have revealed that this leukocyte subset is highly adaptable and fulfills diverse roles. Of special interest is how these adaptations can impact the outcome of an immune response in the lungs due to their potent capacity for clearing infection and causing damage to host tissue. The adaptability of neutrophils and their propensity to influence the outcome of immune responses implicates them as a much-needed target of future immunomodulatory therapies. This review highlights the recent advances elucidating the mechanisms of neutrophilic inflammation, with a focus on the lung environment due to the immense and growing public health burden of chronic lung diseases such as cystic fibrosis (CF) and chronic obstructive pulmonary disease (COPD), and acute lung inflammatory diseases such as transfusion-related acute lung injury (TRALI).
Deletion of the Nucleotide Exchange Factor Vav3 Enhances Axonal Complexity and Synapse Formation but Tampers Activity of Hippocampal Neuronal Networks In Vitro
Authors: Wegrzyn David , Wegrzyn Christine , Tedford Kerry , Fischer Klaus-Dieter , Faissner Andreas
Published: January 2020

Vav proteins activate GTPases of the RhoA subfamily that regulate the cytoskeleton and are involved in adhesion, migration, differentiation, polarity and the cell cycle. While the importance of RhoA GTPases for neuronal morphology is undisputed, their regulation is less well understood. In this perspective, we studied the consequences of the deletion of Vav2, Vav3 and Vav2 and 3 (Vav2−/−, Vav3−/−, Vav2−/−/3−/−) for the development of embryonic hippocampal neurons in vitro. Using an indirect co-culture system of hippocampal neurons with primary wild-type (WT) cortical astrocytes, we analysed axonal and dendritic parameters, structural synapse numbers and the spontaneous network activity via immunocytochemistry and multielectrode array analysis (MEA). Here, we observed a higher process complexity in Vav3−/−, but not in Vav2−/− neurons after three and five days in vitro (DIV). Furthermore, an enhanced synapse formation was observed in Vav3−/− after 14 days in culture. Remarkably, Vav2−/−/3−/− double knockout neurons did not display synergistic effects. Interestingly, these differences were transient and compensated after a cultivation period of 21 days. Network analysis revealed a diminished number of spontaneously occurring action potentials in Vav3−/− neurons after 21 DIV. Based on these results, it appears that Vav3 participates in key events of neuronal differentiation.
Protective Effects of Zerumbone on Colonic Tumorigenesis in Enterotoxigenic Bacteroides fragilis (ETBF)-Colonized AOM/DSS BALB/c Mice
Authors: Hwang Soonjae , Jo Minjeong , Hong Ju Eun , Park Chan Oh , Lee Chang Gun , Rhee Ki-Jong
Published: January 2020

Chronic inflammation has been linked to colitis-associated colorectal cancer in humans. The human symbiont enterotoxigenic Bacteroides fragilis (ETBF), a pro-carcinogenic bacterium, has the potential to initiate and/or promote colorectal cancer. Antibiotic treatment of ETBF has shown promise in decreasing colonic polyp formation in murine models of colon cancer. However, there are no reported natural products that have shown efficacy in decreasing polyp burden. In this study, we investigated the chemopreventive effects of oral administration of zerumbone in ETBF-colonized mice with azoxymethane (AOM)/dextran sulfate sodium (DSS)-induced tumorigenesis. Zerumbone significantly reduced the severity of disease activity index (DAI) scores as well as several parameters of colonic inflammation (i.e., colon weight, colon length, cecum weight and spleen weight). In addition, inflammation of the colon and cecum as well as hyperplasia was reduced. Zerumbone treatment significantly inhibited colonic polyp numbers and prevented macroadenoma progression. Taken together, these findings suggest that oral treatment with zerumbone inhibited ETBF-promoted colon carcinogenesis in mice indicating that zerumbone could be employed as a promising protective agent against ETBF-mediated colorectal cancer.
Azalomycin F5a Eradicates Staphylococcus aureus Biofilm by Rapidly Penetrating and Subsequently Inducing Cell Lysis
Authors: Yuan Ganjun , Li Pingyi , Xu Xuejie , Li Peibo , Zhong Qiwang , He Su , Yi Houqin , Yi Wenfang , Guan Yingying , Wen Zezhang
Published: January 2020

Antimicrobial resistance has emerged as a serious threat to public health. Bacterial biofilm, as a natural lifestyle, is a major contributor to resistance to antimicrobials. Azalomycin F5a, a natural guanidine-containing polyhydroxy macrolide, has remarkable activities against Gram-positive bacteria, including Staphylococcus aureus, a major causative agent of hospital-acquired infections. To further evaluate its potential to be developed as a new antimicrobial agent, its influence on S. aureus biofilm formation was evaluated using the crystal violet method, and then its eradication effect against mature biofilms was determined by confocal laser scanning microscopy, the drop plate method, and regrowth experiments. The results showed that azalomycin F5a could significantly inhibit S. aureus biofilm formation, and such effects were concentration dependent. In addition, it can also eradicate S. aureus mature biofilms with the minimum biofilm eradication concentration of 32.0 μg/mL. As extracellular deoxyribonucleic acid (eDNA) plays important roles in the structural integrity of bacterial biofilm, its influence on the eDNA release in S. aureus biofilm was further analyzed using gel electrophoresis. Combined with our previous works, these results indicate that azalomycin F5a could rapidly penetrate biofilm and causes damages to the cell membrane, leading to an increase in DNase release and eventually eradicating S. aureus biofilm.
Genome-Wide Characterization and Analysis of CIPK Gene Family in Two Cultivated Allopolyploid Cotton Species: Sequence Variation, Association with Seed Oil Content, and the Role of GhCIPK6
Authors: Cui Yupeng , Su Ying , Wang Junjuan , Jia Bing , Wu Man , Pei Wenfeng , Zhang Jinfa , Yu Jiwen
Published: January 2020

Calcineurin B-like protein-interacting protein kinases (CIPKs), as key regulators, play an important role in plant growth and development and the response to various stresses. In the present study, we identified 80 and 78 CIPK genes in the Gossypium hirsutum and G. barbadense, respectively. The phylogenetic and gene structure analysis divided the cotton CIPK genes into five groups which were classified into an exon-rich clade and an exon-poor clade. A synteny analysis showed that segmental duplication contributed to the expansion of Gossypium CIPK gene family, and purifying selection played a major role in the evolution of the gene family in cotton. Analyses of expression profiles showed that GhCIPK genes had temporal and spatial specificity and could be induced by various abiotic stresses. Fourteen GhCIPK genes were found to contain 17 non-synonymous single nucleotide polymorphisms (SNPs) and co-localized with oil or protein content quantitative trait loci (QTLs). Additionally, five SNPs from four GhCIPKs were found to be significantly associated with oil content in one of the three field tests. Although most GhCIPK genes were not associated with natural variations in cotton oil content, the overexpression of the GhCIPK6 gene reduced the oil content and increased C18:1 and C18:1+C18:1d6 in transgenic cotton as compared to wild-type plants. In addition, we predicted the potential molecular regulatory mechanisms of the GhCIPK genes. In brief, these results enhance our understanding of the roles of CIPK genes in oil synthesis and stress responses.
Reduced miR-26b Expression in Megakaryocytes and Platelets Contributes to Elevated Level of Platelet Activation Status in Sepsis
Authors: Szilágyi Bernadett , Fejes Zsolt , Póliska Szilárd , Pócsi Marianna , Czimmerer Zsolt , Patsalos Andreas , Fenyvesi Ferenc , Rusznyák Ágnes , Nagy György , Kerekes György , Berhés Mariann , Szűcs Ildikó , Kunapuli Satya P. , Kappelmayer János , Nagy Béla
Published: January 2020

In sepsis, platelets may become activated via toll-like receptors (TLRs), causing microvascular thrombosis. Megakaryocytes (MKs) also express these receptors; thus, severe infection may modulate thrombopoiesis. To explore the relevance of altered miRNAs in platelet activation upon sepsis, we first investigated sepsis-induced miRNA expression in platelets of septic patients. The effect of abnormal Dicer level on miRNA expression was also evaluated. miRNAs were profiled in septic vs. normal platelets using TaqMan Open Array. We validated platelet miR-26b with its target SELP (P-selectin) mRNA levels and correlated them with clinical outcomes. The impact of sepsis on MK transcriptome was analyzed in MEG-01 cells after lipopolysaccharide (LPS) treatment by RNA-seq. Sepsis-reduced miR-26b was further studied using Dicer1 siRNA and calpain inhibition in MEG-01 cells. Out of 390 platelet miRNAs detected, there were 121 significantly decreased, and 61 upregulated in sepsis vs. controls. Septic platelets showed attenuated miR-26b, which were associated with disease severity and mortality. SELP mRNA level was elevated in sepsis, especially in platelets with increased mean platelet volume, causing higher P-selectin expression. Downregulation of Dicer1 generated lower miR-26b with higher SELP mRNA, while calpeptin restored miR-26b in MEG-01 cells. In conclusion, decreased miR-26b in MKs and platelets contributes to an increased level of platelet activation status in sepsis.
GM1 Ganglioside Is A Key Factor in Maintaining the Mammalian Neuronal Functions Avoiding Neurodegeneration
Authors: Chiricozzi Elena , Lunghi Giulia , Di Biase Erika , Fazzari Maria , Sonnino Sandro , Mauri Laura
Published: January 2020

Many species of ganglioside GM1, differing for the sialic acid and ceramide content, have been characterized and their physico-chemical properties have been studied in detail since 1963. Scientists were immediately attracted to the GM1 molecule and have carried on an ever-increasing number of studies to understand its binding properties and its neurotrophic and neuroprotective role. GM1 displays a well balanced amphiphilic behavior that allows to establish strong both hydrophobic and hydrophilic interactions. The peculiar structure of GM1 reduces the fluidity of the plasma membrane which implies a retention and enrichment of the ganglioside in specific membrane domains called lipid rafts. The dynamism of the GM1 oligosaccharide head allows it to assume different conformations and, in this way, to interact through hydrogen or ionic bonds with a wide range of membrane receptors as well as with extracellular ligands. After more than 60 years of studies, it is a milestone that GM1 is one of the main actors in determining the neuronal functions that allows humans to have an intellectual life. The progressive reduction of its biosynthesis along the lifespan is being considered as one of the causes underlying neuronal loss in aged people and severe neuronal decline in neurodegenerative diseases. In this review, we report on the main knowledge on ganglioside GM1, with an emphasis on the recent discoveries about its bioactive component.
Roles of Brassinosteroids in Plant Reproduction
Authors: Li Zicong , He Yuehui
Published: January 2020

Brassinosteroids (BRs) are a group of steroid hormones, essentially important for plant development and growth. BR signaling functions to promote cell expansion and cell division, and plays a role in etiolation and reproduction. As the phytohormone originally identified in the pollen grains of Brassica napus, BR promotes the elongation of stigma. Recent studies have revealed that BR is also critical for floral transition, inflorescence stem architecture formation and other aspects of plant reproductive processes. In this review, we focus on the current understanding of BRs in plant reproduction, the spatial and temporal control of BR signaling, and the downstream molecular mechanisms in both the model plant Arabidopsis and crops. The crosstalk of BR with environmental factors and other hormones in reproduction will also be discussed.
Theme Mapping and Bibliometrics Analysis of One Decade of Big Data Research in the Scopus Database
Authors: Parlina Anne , Ramli Kalamullah , Murfi Hendri
Published: January 2020

Recently, the popularity of big data as a research field has shown continuous and wide-scale growth. This study aims to capture the scientific structure and topic evolution of big data research using bibliometrics and text mining-based analysis methods. Bibliographic data of journal articles regarding big data published between 2009 to 2018 were collected from the Scopus database and analyzed. The results show a significant growth of publications since 2014. Furthermore, the findings of this study highlight the core journals, most cited articles, top productive authors, countries, and institutions. Secondly, a unique approach to identifying and analyzing major research themes in big data publications was proposed. Keywords were clustered, and each cluster was labeled as a theme. Moreover, the papers were divided into four sub-periods to observe the thematic evolution. The theme mapping reveals that research on big data is dominated by big data analytics, which covers methods, tools, supporting infrastructure, and applications. Other critical aspects of big data research are security and privacy. Social networks and the Internet of things are significant sources of big data, and the resources and services offered by cloud computing strongly support the management and processing of big data.
Designing Internet of Tangible Things for Children with Hearing Impairment
Authors: Cano Sandra , Peñeñory Victor , Collazos César A. , Albiol-Pérez Sergio
Published: January 2020

Tangible User Interfaces (TUIs) are a new, non-traditional way to interact with digital information using a physical environment. Therefore, TUIs connect a physical set of objects that can be explored and manipulated. TUI can be interconnected over the Internet, using Internet of Things (IoT) technology to monitor a child’s activities in real-time. Internet of Tangible Things (IoTT) is defined as a tangible interaction applied to IoT. This article describes four case studies that apply IoTT to children with cochlear implants and children whose communication is sign language. For each case study, a discussion is presented, discussing how IoTT can help the child development in skills such as: social, emotional, psychomotor, cognitive, and visual. It was found that IoTT works best when it includes the social component in children with hearing impairment, because it helps them to communicate with each other and build social-emotional skills.
Cross-Generation Analysis of e-Book Consumers’ Preferences—A Prerequisite for Effective Management of Public Library
Authors: Prokop Viktor , Stejskal Jan
Published: January 2020

Nowadays, with the growing importance of providing digital services, public libraries offer a wide range of e-books. However, current studies show that reading preferences vary across countries, and identifying e-book demand is therefore difficult. For these reasons, this article focuses on different groups of e-book readers (pensioners, students, economically active) and on factors affecting their attitude to reading e-books, willingness to receive new library services and willingness to pay for them. For the purpose of this analysis, we are using unique data from an online questionnaire survey among readers of the Municipal Library of Prague in 2019 and own binary logistic models. The results show how e-book genres, information preferences and devices for reading e-books primarily affect selected groups of e-book readers. The unwillingness of e-book readers to pay for library services is also evident, especially among students. In the last section, we propose some practical implications that could help to library management and possibly attract more e-book readers from different groups.
Proceedings of the 2019 American Bee Research Conference
Authors: Simone-Finstrom Michael , Niño Elina L. , Flenniken Michelle L. , Arrowsmith Hanna , Wu-Smart Judy
Published: January 2020

The 2019 American Bee Research Conference (ABRC) was held January 10–12, 2019 in conjunction with the annual convention of the American Honey Producers Association in Tempe, AZ. Over the three-day conference, a total of 45 oral presentations and 13 poster presentations were given, representing work done from over 27 institutions and 34 different research groups from throughout the United States and Canada. This proceedings contains and overview of the conference and the submitted abstracts for presentations given at the 2018 American Bee Research Conference.
Retinoic Acids in the Treatment of Most Lethal Solid Cancers
Authors: Costantini Lara , Molinari Romina , Farinon Barbara , Merendino Nicolò
Published: January 2020

Although the use of oral administration of pharmacological all-trans retinoic acid (ATRA) concentration in acute promyelocytic leukaemia (APL) patients was approved for over 20 years and used as standard therapy still to date, the same use in solid cancers is still controversial. In the present review the literature about the top five lethal solid cancers (lung, stomach, liver, breast, and colon cancer), as defined by The Global Cancer Observatory of World Health Organization, and retinoic acids (ATRA, 9-cis retinoic acid, and 13-cis retinoic acid, RA) was compared. The action of retinoic acids in inhibiting the cell proliferation was found in several cell pathways and compartments: from membrane and cytoplasmic signaling, to metabolic enzymes, to gene expression. However, in parallel in the most aggressive phenotypes several escape routes have evolved conferring retinoic acids-resistance. The comparison between different solid cancer types pointed out that for some cancer types several information are still lacking. Moreover, even though some pathways and escape routes are the same between the cancer types, sometimes they can differently respond to retinoic acid therapy, so that generalization cannot be made. Further studies on molecular pathways are needed to perform combinatorial trials that allow overcoming retinoic acids resistance.
Cognitive Improvement in Older Adults with Mild Cognitive Impairment: Evidence from a Multi-Strategic Metamemory Training
Authors: Youn Jung-Hae , Park Soowon , Lee Jun-Young , Cho Seong-Jin , Kim Jeongsim , Ryu Seung-Ho
Published: January 2020

Intervention programs to relieve memory impairment and memory-related complaints in older adults with mild cognitive impairment are needed. Objective: The purpose of the current study was to assess the efficacy of a novel cognitive training approach—named multi-strategic metamemory training—in older adults with amnestic mild cognitive impairment. Among a total of 113 older adults with mild cognitive impairment, 66 participated in the memory training program (training group) and 47 did not (control group). Repeated measures of analysis of variance revealed that compared with the control group, the training group experienced: (i) a significantly greater increase in cognitive test scores of long-term delayed free recall (Finteraction = 6.04, p = 0.016) and fluency (Finteraction = 4.11, p = 0.045) and (ii) significantly greater decrease in their subjective memory complaints for everyday memory (Finteraction = 7.35, p = 0.009). These results suggest that the training program can improve verbal memory (i.e., delayed free recall), language processing (i.e., categorical fluency) and limit complaints in everyday instrumental memory activities of mildly impaired older adults.
Evaluation of Sperm Mitochondrial Function: A Key Organelle for Sperm Motility
Authors: Barbagallo Federica , La Vignera Sandro , Cannarella Rossella , Aversa Antonio , Calogero Aldo E. , Condorelli Rosita A.
Published: January 2020

Introduction: The role of nutraceuticals in the treatment of male infertility, especially in the “idiopathic form”, remains the subject of significant debate. Many antioxidants improve sperm motility but the exact mechanism by which they act is still unclear. Although several studies have shown a correlation between sperm motility and mitochondrial function, the effects of antioxidant therapy on mitochondrial membrane potential (MMP) are poorly studied. The first aim of this review was to evaluate the efficacy of antioxidants on mitochondrial function and, consequently, on sperm motility in male infertile patients. Material and Methods: we performed a systematic search of all randomized controlled and uncontrolled studies available in the literature that reported sperm motility and MMP at baseline and after antioxidant administration in-vivo and in-vitro in patients with idiopathic asthenozoospermia. Pubmed, MEDLINE, Cochrane, Academic One Files, Google Scholar and Scopus databases were used. Results: Unexpectedly, among 353 articles retrieved, only one study met our inclusion criteria and showed a significant effect of myoinositol on both MMP and sperm motility. We then summarized the main knowledge on anatomy and metabolism of sperm mitochondria, techniques allowing to assess sperm mitochondria function and its relationships with low sperm motility. Finally, we paid special attention to the effect of antioxidant/prokinetic molecules for the treatment of asthenozoospermia. Conclusions: This is the first systematic review that has attempted to evaluate the effects of antioxidants on MMP and sperm motility. Although results are not conclusive due to the dearth of studies, the close relationship between mitochondria and sperm motility is clear. The investigation of this correlation could provide valuable information to be exploited in clinical practice for the treatment of male infertility.
Validation of Deep Learning-Based Artifact Correction on Synthetic FLAIR Images in a Different Scanning Environment
Authors: Ryu Kyeong Hwa , Baek Hye Jin , Gho Sung-Min , Ryu Kanghyun , Kim Dong-Hyun , Park Sung Eun , Ha Ji Young , Cho Soo Buem , Lee Joon Sung
Published: January 2020

We investigated the capability of a trained deep learning (DL) model with a convolutional neural network (CNN) in a different scanning environment in terms of ameliorating the quality of synthetic fluid-attenuated inversion recovery (FLAIR) images. The acquired data of 319 patients obtained from the retrospective review were used as test sets for the already trained DL model to correct the synthetic FLAIR images. Quantitative analyses were performed for native synthetic FLAIR and DL-FLAIR images against conventional FLAIR images. Two neuroradiologists assessed the quality and artifact degree of the native synthetic FLAIR and DL-FLAIR images. The quantitative parameters showed significant improvement on DL-FLAIR in all individual tissue segments and total intracranial tissues than on the native synthetic FLAIR (p < 0.0001). DL-FLAIR images showed improved image quality with fewer artifacts than the native synthetic FLAIR images (p < 0.0001). There was no significant difference in the preservation of the periventricular white matter hyperintensities and lesion conspicuity between the two FLAIR image sets (p = 0.217). The quality of synthetic FLAIR images was improved through artifact correction using the trained DL model on a different scan environment. DL-based correction can be a promising solution for ameliorating the quality of synthetic FLAIR images to broaden the clinical use of synthetic magnetic resonance imaging (MRI).
Analysis of De Novo Mutations in Sporadic Cardiomyopathies Emphasizes Their Clinical Relevance and Points to Novel Candidate Genes
Authors: Franaszczyk Maria , Truszkowska Grazyna , Chmielewski Przemyslaw , Rydzanicz Malgorzata , Kosinska Joanna , Rywik Tomasz , Biernacka Anna , Spiewak Mateusz , Kostrzewa Grazyna , Stepien-Wojno Malgorzata , Stawinski Piotr , Bilinska Maria , Krajewski Pawel , Zielinski Tomasz , Lutynska Anna , Bilinska Zofia T. , Ploski Rafal
Published: January 2020

The vast majority of cardiomyopathies have an autosomal dominant inheritance; hence, genetic testing is typically offered to patients with a positive family history. A de novo mutation is a new germline mutation not inherited from either parent. The purpose of our study was to search for de novo mutations in patients with cardiomyopathy and no evidence of the disease in the family. Using next-generation sequencing, we analyzed cardiomyopathy genes in 12 probands. In 8 (66.7%), we found de novo variants in known cardiomyopathy genes (TTN, DSP, SCN5A, TNNC1, TPM1, CRYAB, MYH7). In the remaining probands, the analysis was extended to whole exome sequencing in a trio (proband and parents). We found de novo variants in genes that, so far, were not associated with any disease (TRIB3, SLC2A6), a possible disease-causing biallelic genotype (APOBEC gene family), and a de novo mosaic variant without strong evidence of pathogenicity (UNC45A). The high prevalence of de novo mutations emphasizes that genetic screening is also indicated in cases of sporadic cardiomyopathy. Moreover, we have identified novel cardiomyopathy candidate genes that are likely to affect immunological function and/or reaction to stress that could be especially relevant in patients with disease onset associated with infection/infestation.
Thermal Modification Effect on Supported Cu-Based Activated Carbon Catalyst in Hydrogenolysis of Glycerol
Authors: Seguel Juan , García Rafael , Chimentão Ricardo José , García-Fierro José Luis , Ghampson I. Tyrone , Escalona Néstor , Sepúlveda Catherine
Published: January 2020

Glycerol hydrogenolysis to 1,2-propanediol (1,2-PDO) was performed over activated carbon supported copper-based catalysts. The catalysts were prepared by impregnation using a pristine carbon support and thermally-treated carbon supports (450, 600, 750, and 1000 °C). The final hydrogen adsorption capacity, porous structure, and total acidity of the catalysts were found to be important descriptors to understand catalytic performance. Oxygen surface groups on the support controlled copper dispersion by modifying acidic and adsorption properties. The amount of oxygen species of thermally modified carbon supports was also found to be a function of its specific surface area. Carbon supports with high specific surface areas contained large amount of oxygen surface species, inducing homogeneous distribution of Cu species on the carbon support during impregnation. The oxygen surface groups likely acted as anchorage centers, whereby the more stable oxygen surface groups after the reduction treatment produced an increase in the interaction of the copper species with the carbon support, and determined catalytic performances.
Experimental Study of Cement Alkali-Resistant Glass Fiber (C-ARGF) Grouting Material
Authors: Shi Zhenyue , Wang Qingbiao , Xu Lei
Published: January 2020

Mixing alkali-resistant glass fiber (ARGF) into grouting slurry can prevent the development of cracks; thus, understanding the properties of ARGF grouting material is important for applications in engineering. Two types of ARGFs (Cem-FIL®60 and Anti-Crak®HD) were selected as mixing materials, and their performance was tested in four areas, namely, compressive strength, tensile strength, flexural strength, and impervious performance, under four different mixing amounts of fiber (0%, 0.25%, 0.5%, and 1.0%). Results demonstrate that the addition of ARGF increased the compressive strength and tensile strength of the grouting slurry, and the best performance was at 0.5%. The effect on the flexural strength and impervious performance was related to the mixing amount, and the fiber may have induced a counter-effect for certain amounts of added ARGF. Mixing ARGF could increase the early strength ratio of grout; however, a high early strength ratio did not necessarily result in high strength, as the flexural strength did not change synchronously with the early strength ratio; a similar pattern was found for the impermeability. Cem-FIL®60 had a better effect on the properties of grouting materials than Anti-Crak®HD. These results were successfully applied in the water-plugging and reinforcement engineering of a karst tunnel.
Microstructures and Mechanical Properties of Laser-Sintered Commercially Pure Ti and Ti-6Al-4V Alloy for Dental Applications
Authors: Okazaki Yoshimitsu , Ishino Akira
Published: January 2020

To apply laser-sintered titanium (Ti) materials to dental prostheses with a three-dimensional structure such as partial dentures, we examined the microstructures and mechanical properties of commercially pure (CP) Ti grade (G) 2 annealed after laser sintering and laser-sintered (as-built) Ti-6Al-4V alloy. The tensile and fatigue properties of CP Ti G 2 annealed at 700 °C for 2 h after laser sintering were close to those of wrought CP Ti G 2 annealed at the same temperature after hot forging. The ultimate tensile strengths (σUTS) of 90°- and 0°-direction-built CP Ti G 2 rods after laser sintering 10 times were 553 and 576 MPa and the total elongations (TE) of these rods were 26% and 28%, respectively. The fatigue strengths (σFS) at 107 cycles of the 90°- and 0°-direction-built CP Ti G 2 rods after laser sintering 10 times were ~320 and ~365 MPa, respectively. The ratio σFS/σUTS was in the range of 0.5–0.7. The changes in the chemical composition and mechanical properties after laser sintering 10 times were negligible. The fatigue strength of the laser-sintered Ti-6Al-4V alloy was ~600 MPa, which was close to that of wrought Ti-6Al-4V alloy. These findings indicate that the laser-sintered CP Ti and Ti-6Al-4V alloy can also be applied in dental prostheses similarly to laser-sintered Co–Cr–Mo alloy. In particular, it was clarified that laser sintering using CP Ti G 4 powder is useful for dental prostheses.
Development of a Knee Joint CT-FEM Model in Load Response of the Stance Phase During Walking Using Muscle Exertion, Motion Analysis, and Ground Reaction Force Data
Authors: Watanabe Kunihiro , Mutsuzaki Hirotaka , Fukaya Takashi , Aoyama Toshiyuki , Nakajima Syuichi , Sekine Norio , Mori Koichi
Published: January 2020

Background and objectives: There are no reports on articular stress distribution during walking based on any computed tomography (CT)-finite element model (CT-FEM). This study aimed to develop a calculation model of the load response (LR) phase, the most burdensome phase on the knee, during walking using the finite element method of quantitative CT images. Materials and Methods: The right knee of a 43-year-old man who had no history of osteoarthritis or surgeries of the knee was examined. An image of the knee was obtained using CT and the extension position image was converted to the flexion angle image in the LR phase. The bone was composed of heterogeneous materials. The ligaments were made of truss elements; therefore, they do not generate strain during expansion or contraction and do not affect the reaction force or pressure. The construction of the knee joint included material properties of the ligament, cartilage, and meniscus. The extensor and flexor muscles were calculated and set as the muscle exercise tension around the knee joint. Ground reaction force was vertically applied to suppress the rotation of the knee, and the thigh was restrained. Results: An FEM was constructed using a motion analyzer, floor reaction force meter, and muscle tractive force calculation. In a normal knee, the equivalent stress and joint contact reaction force in the LR phase were distributed over a wide area on the inner upper surface of the femur and tibia. Conclusions: We developed a calculation model in the LR phase of the knee joint during walking using a CT-FEM. Methods to evaluate the heteromorphic risk, mechanisms of transformation, prevention of knee osteoarthritis, and treatment may be developed using this model.