bluuebunny/crossref_metadata_2025 huggingface dataset:
Impact of the COVID-19 Pandemic on Tobacco Sales and National Smoking Cessation Services in Korea
Authors: Kim Jinyoung , Lee Sungkyu
Published: April 2022

This study aimed to describe the effect of the COVID-19 pandemic, combined with the Korean government’s response to the pandemic on tobacco consumption and national smoking cessation services among the Korean population. We obtained tobacco sale data from the Ministry of Finance and analysed the data on smokers’ visits to national smoking cessation clinics during the pandemic from a member of the National Assembly. We also conducted an online search to understand smokers’ thoughts about their tobacco use during the pandemic. We found that after the emergence of COVID-19 in 2020, the sale of conventional cigarettes increased from 3063.70 to 3209.70 million packs (4.77%). The number of smokers who visited clinics sharply decreased in the first half of 2020. The six-month quit rate decreased from 38.5% in 2017 to 22.3% in early 2020. We also found that smokers increased their consumption and began to switch from conventional cigarettes to heated tobacco products. The COVID-19 pandemic has threatened tobacco control policies and programs in Korea in the last two years; however, based on our experience during this period and considering the WHO recommendation, we should sustain and reinforce tobacco control policies and national smoking cessation services today and in the future.
Assessing Virtual Mental Health Access for Refugees during the COVID-19 Pandemic Using the Levesque Client-Centered Framework: What Have We Learned and How Will We Plan for the Future?
Authors: Hynie Michaela , Jaimes Annie , Oda Anna , Rivest-Beauregard Marjolaine , Perez Gonzalez Laura , Ives Nicole , Ahmad Farah , Kuo Ben C. H. , Arya Neil , Bokore Nimo , McKenzie Kwame
Published: April 2022

During the COVID-19 pandemic, mental health services rapidly transitioned to virtual care. Although such services can improve access for underserved populations, they may also present unique challenges, especially for refugee newcomers. This study examined the multidimensional nature of access to virtual mental health (VMH) care for refugee newcomers during the COVID-19 pandemic, using Levesque et al.’s Client-Centered Framework for Assessing Access to Health Care. One hundred and eight structured and semi structured interviews were conducted in four Canadian provinces (8 community leaders, 37 newcomer clients, 63 mental health or service providers or managers). Deductive qualitative analysis, based on the Client-Centered Framework, identified several overarching themes: challenges due to the cost and complexity of using technology; comfort for VMH outside clinical settings; sustainability post-COVID-19; and communication and the therapeutic alliance. Mental health organizations, community organizations, and service providers can improve access to (virtual) mental health care for refugee newcomers by addressing cultural and structural barriers, tailoring services, and offering choice and flexibility to newcomers.
Pediatric Cancer as a Factor of Changes in the Family
Authors: Dąbrowska Aleksandra , Malicka Iwona
Published: April 2022

The occurrence of pediatric cancer is an example of a non-normative situation that reorganizes family life. The aim of the study was to evaluate the functioning of a family with a child affected by cancer. The study was conducted on 339 families. The study group consisted of 153 families with children with cancer (mean age 36.4 ± 6.8 years). The control group was composed of 186 families with healthy children (mean age 39.0 ± 6.3 years). All of them completed the author’s survey questionnaire on family functioning and the Resilience Measurement Scale (RMS). A statistically significant association was found between the place of residence (p < 0.001), education (p < 0.001), assessment of the material status (p < 0.001) and employment structure (p < 0.001) of parents and the membership in the study group or the control group. Statistical significance was achieved for the main effects as measured on the RMS (5 factors and 2 groups). The study group showed consistently lower levels of the factors of the scale. Statistically significant observations were reported for Factor 1 (perseverance, proactive approach) and Factor 4 (tolerance to failure, life as a challenge) (12.0 vs. 14.5, p < 0.001, 13.4 vs. 14.2, p = 0.04, respectively). Parents of children with cancer were characterized by lower persistence, determination and tolerance to failure, which could affect the quality of life of the whole family. In addition, different socio-economic conditions of family functioning were found in families with children with cancer as compared to families with healthy children.
Therapeutic Environments in Drug Treatment: From Stigmatising Spaces to Enabling Places. A Theory-Based Qualitative Analysis
Authors: Bank Mads , Roessler Kirsten K.
Published: April 2022

Investigating therapeutic environments for young drug users is needed to avoid a high dropout rate due to a potential stigmatising effect of the structure of the space. In this article, we draw from three semi-structured interviews with young drug users. The interviews focused broadly on their experiences being on drug treatment and on how they experienced counselling and treatment in different spaces. The findings show that therapeutic spaces that were viewed as clinical and sterile were experienced as stigmatising, which discouraged young drug users from engaging in treatment and therapeutic processes. In contrast, therapeutic places with a homely atmosphere reduced the experience of stigmatisation, facilitated participation in treatment and helped users to relax and feel part of a community. In the analysis, we show how enabling therapeutic places with a homely atmosphere can be produced through materials, activities, and sensory processes.
Comparison of Study Quality as Determined by Standard Research and Community Engagement Metrics: A Pilot Study on Breast Cancer Research in Urban, Rural, and Remote Indigenous Communities
Authors: Christie Vita , Amin Janaki , Skinner John , Green Debbie , Littlejohn Karen , Gwynne Kylie
Published: April 2022

The purpose of this review is to compare research evaluation tools to determine whether the tools typically used for assessing the quality of research adequately address issues of Indigenous health and culture, particularly when the studies are intended to benefit Indigenous peoples in urban, regional, rural, and remote settings. Our previously published systematic review evaluated studies about breast cancer using a modified Indigenous community engagement tool (CET). In this study, we evaluated the same studies using two commonly used tools: the Critical Appraisal Skills Programme (CASP) for qualitative research; and the Effective Public Health Practice Project (EPHPP) for quantitative research. The results were then compared to ascertain whether there was alignment between performances in terms of engagement and the CASP/EPHPP metrics. Of the 15 papers, 3 papers scored weakly on both metrics, and are therefore the least likely to offer reliable findings, while 2 papers scored strongly on both metrics, and are therefore the most likely to offer reliable findings. Beyond this summation, it was clear that the results did not align and, therefore, could not be used interchangeably when applied to research findings intended to benefit Indigenous peoples. There does not appear to be a pattern in the relationship between the reliability of the studies and the study settings. In order to address disparities in health outcomes, we must assess research through a typical research quality and cultural engagement and settings lens, ensuring that there is rigour in all aspects of the studies.
Stress in Medical Students: PRIMES, an Italian, Multicenter Cross-Sectional Study
Authors: Leombruni Paolo , Corradi Alessio , Lo Moro Giuseppina , Acampora Anna , Agodi Antonella , Celotto Daniele , Chironna Maria , Cocchio Silvia , Cofini Vincenza , D’Errico Marcello Mario , Marzuillo Carolina , Pavia Maria , Restivo Vincenzo , Veronesi Licia , Gualano Maria Rosaria , Bert Fabrizio , Siliquini Roberta ,   on behalf of the PRIMES Collaborating Group
Published: April 2022

Medical students (MSs) are healthcare workers and must also cope with education-related stressors. This study aims to assess factors associated with perceived stress in Italian MSs. A cross-sectional study was conducted in 12 Italian medical schools (MSCs) in November 2018. A questionnaire assessed socio-demographic characteristics, habits, opinions about MSC, and concerns about the future. Stress was assessed with the PSS-10. Descriptive and univariable tests were performed. A linear model was fitted to find associations of the PSS-10 score with characteristics. There were 2513 collected questionnaires. Median PSS-10 score was 18 (IQR 11). Median age was 22 (IQR 4) and 61% of the sample was female. Female gender, being part of a sexual minority, poor financial situation, competitive atmosphere, having hobbies, resting, and sleeping hindered by MSC were characteristics associated with higher PSS-10 scores. Current relationship, good family relationship, and no concerns about the future were protective factors. Being part of sexual minorities had greater effects in students not living away from home, while in the other group it was not having satisfying friendships. Medical students suffer higher levels of stress than the general population, and many MSC stressors are associated. Living away from home modifies risk and protective factors, offering the possibility to tailor group-specific interventions.
Current Status and Trends in Research on Caries Diagnosis: A Bibliometric Analysis
Authors: Melo María , Sanz José Luis , Forner Leopoldo , Rodríguez-Lozano Francisco Javier , Guerrero-Gironés Julia
Published: April 2022

There are a wide variety of devices for the detection and diagnosis of caries from the initial stages. The purpose of this study was to perform a bibliometric study on research regarding caries diagnosis by identifying the contributing researchers, organizations, countries or regions, journals, and to provide an analysis of keyword co-occurrence and co-authorship networks. An advanced search was performed in Web of Science (WOS) Core Collection database, using the terms “caries diagno*” and “caries detect*” in the “topic” field, from 2013 to 2021. Bibliometric parameters were extracted using WOS’s analyze results tools and VOSviewer software. A total of 816 documents were identified. Most of them, (61.3%) are included in “Dentistry Oral Surgery & Medicine” category within WOS. The largest scientific production on the subject is observed between 2018 and 2021, with a total of 344 records. The most productive author is Mendes FM, followed by Braga MM. The journal with the most articles published on caries diagnosis is Caries Research, with 55 articles (6.74%). The terms with the highest co-occurrence refer to the validity of diagnostic methods, tools or principles used in diagnosis or general aspects related to caries detection and diagnosis.
Parenting Intervention for Psychological Flexibility and Emotion Regulation: Clinical Protocol and an Evidence-Based Case Study
Authors: Flujas-Contreras Juan M. , García-Palacios Azucena , Gómez Inmaculada
Published: April 2022

Psychological flexibility has been found as a protective factor for several psychological problems, including the field of parenting. The present study aims to illustrate a clinical protocol, session by session, for the promotion of parental psychological flexibility and emotion regulation in a case study. The clinical protocol is based on third-wave behavior therapy in a brief intervention of four sessions. The intervention is presented in a clinical case of a mother with a child diagnosed with Oppositional Defiant Disorder. Both mother and child experienced problems with emotional regulation and psychological flexibility. The results show clinically significant improvements in psychological flexibility, emotional regulation, and stress parenting in the mother both after the intervention and at follow-up. In the child, emotional perspective-taking skills, acceptance, and valued actions improved. The case illustrates in detail the application of different strategies of acceptance, mindfulness, emotion regulation, and emotional defusion applicable to parenting. Clinical implications are discussed.
Awareness of Chronic Kidney Disease, Medication, and Laboratory Investigation among Nephrology and Urology Patients of Quetta, Pakistan
Authors: Ahmed Jahanzaib , Azhar Saira , ul Haq Noman , Hussain Sajjad , Stájer Anette , Urbán Edit , Gajdács Márió , Jamshed Shazia
Published: April 2022

Patients’ awareness is critical in medical care, as it can serve as an input into the adjustment of interventions. The aim of study was to explore the level of awareness regarding chronic kidney disease (CKD), its medications, and laboratory investigations among nephrology and urology patients of Quetta. The cross-sectional study was used by adopting and culturally modifying a questionnaire. By convenient sampling technique, a total of 500 questionnaires were self-administered to inpatients, outpatients, and dialysis patients, and 468 responses (response rate 93.6%) were analyzed. Descriptive statistics, inferential statistics, and reliability analysis were performed on SPSS v25. A majority, 50.3% (n = 235), was unaware of symptoms that will develop due to worsening of disease, while 56.2% (n = 263) were unaware of what aggravates their kidney function. Almost half of the affected individuals, 47.4% (n = 222), have no understanding about the long-term prognosis of the disease. The majority of the respondents, 51.5% (n = 248), do not know about the names and usage of medications, and 62.4% (n = 292) were unaware of the medicines that may impair kidney function; more than half, 66.7% (n = 312), were unaware about the necessary laboratory investigations. A strong association between awareness and patient education level was found (p < 0.001). Awareness regarding disease condition, medications, and laboratory investigations of CKD among nephrology and urology patients of Quetta was found out to be low, which needs immediate educational intervention.
Association of Updating Identification Documents with Suicidal Ideation and Attempts among Transgender and Nonbinary Youth
Authors: DeChants Jonah P. , Price Myeshia N. , Green Amy E. , Davis Carrie K. , Pick Casey J.
Published: April 2022

This study examines the association of access to concordant identity documents with attempting suicide in the last year among transgender and nonbinary youth. Data came from 6581 transgender and nonbinary youth who completed an online survey of lesbian, gay, bisexual, transgender, or queer (LGBTQ) youth ages 13–24 residing in the United States. Multivariate logistic regression was used to determine the adjusted odds of attempting suicide in the past year based on whether or not youth were able to change their identification documents. Both wanting to update one’s documents but not being able to (aOR = 2.04, CI = 1.412–2.95; p < 0.001) and being able to update one’s documents but not having done so (aOR = 1.74, CI = 1.22–2.50; p < 0.001) were associated with greater odds of attempting suicide in the last year, compared to youth who had changed their documents. Revising the way gender is captured on legal documents may be an efficacious public health intervention to reduce suicide risk among transgender and nonbinary youth.
The Prevalence of Dietary Supplement Usage in Military Aviators
Authors: Sammito Stefan , Erley Oliver Maria , Rose Dirk-Matthias , Güttler Norbert
Published: April 2022

Background: The prevalence of dietary supplement (DS) and energy drink (ED) usage in military personnel differs from branch to branch and is between 55% and 76% (higher values in special operations forces). Aviators with highly demanding tasks might be especially interested in using dietary supplements. To date, there are only limited data available for this special profession inside the military. Methods: An internet-based survey was conducted on the prevalence of DS and ED usage, the reasons for their usage and the place of purchase for all wings of the German Armed Forces. Results: Of the 181 pilots who participated in the survey, 34% used DSs and 16% EDs. Usage was linked to sports activities but not to the type of aircraft. DSs were purchased on the internet by 50% of the respondents; mostly protein supplements, magnesium and omega-3fatty acids. Only 42% said they would feel an effect from taking DSs. Conclusions: Although the present study showed that the prevalence of usage was comparable to that of the civilian population, the sources of supply and the range of the substances taken give cause for concern. This calls for education and information campaigns to make the pilots aware of the possible risks to their health.
Comment on Bunmaprasert et al. Reducible Nonunited Type II Odontoid Fracture with Atlantoaxial Instability: Outcomes of Two Different Fixation Techniques. Int. J. Environ. Res. Public Health 2021, 18, 7990
Authors: Kanat Ayhan
Published: April 2022

I read with great interest the paper of Bunmaprasert et al. [...]
A Successful New Case of Twin Pregnancy in a Patient with Swyer Syndrome—An Up-to-Date Review on the Incidence and Outcome of Twin/Multiple Gestations in the Pure 46,XY Gonadal Dysgenesis
Authors: Winkler Izabela , Jaszczuk Ilona , Gogacz Marek , Szkodziak Piotr , Paszkowski Tomasz , Skorupska Katarzyna , Ciebiera Michał , Skrzypczak Maciej
Published: April 2022

Background: The aim of the present study is to report a rare occurrence of a successful twin pregnancy in a woman with pure 46,XY gonadal dysgenesis. Result(s): A patient with Swyer syndrome (pure 46,XY gonadal dysgenesis) presented with a twin pregnancy after in vitro fertilization. Due to unidentified conditions, the patient developed selective intrauterine growth restriction in one of the fetuses. Twins were born at 33 weeks of pregnancy due to the risk of asphyxia. Nonetheless, the patient did not develop gonadal malignancies before the pregnancy and, despite receiving estrogen, remained amenorrheic. Conclusion(s): The aim of this case report is to show the course of twin pregnancy in patients with Swyer syndrome through assisted reproduction. Due to certain disorders in the development of their reproductive organs, such as the less mature uterus, such pregnancies may be associated with an increased risk. The above case report demonstrates the need to systematize methods of pregnancy management in patients with Swyer syndrome, such as: preparation for the pregnancy, assessment of the uterus, medications used, and necessary checkups. Capsule: This case report and review shows clinicians that patients with Swyer syndrome may become pregnant. Twin pregnancies may occur without any major problems through assisted reproduction.
Healthy Community-Life Circle Planning Combining Objective Measurement and Subjective Evaluation: Theoretical and Empirical Research
Authors: Wan Jiangjun , Zhao Yutong , Zhang Kaili , Ma Chunchi , Sun Haiying , Wang Ziming , Wu Hongyu , Li Mingjie , Zhang Lingqing , Tang Xiaohong , Cao Ying , Tang Li , Yang Jinxiu
Published: April 2022

Background: The world faces vast health challenges, and urban residents living in high-density areas have even greater demand for healthy lifestyles. Methods: Based on the data of points of interest, a field survey, and an interview, we explored the healthy community-life circle in the downtown area of Chengdu, China from two perspectives: objective measurement and subjective perception of residents. We evaluated the coverage rate and convenience in accessing eight types of health service facilities within a 15-min walk using linear and logistics regression models to explore the degree of resident satisfaction with facilities and influencing factors. Results: Results showed significant differences in coverage rates between different districts. The overall convenience in accessing health service facilities decreased gradually from the city center to the outskirts. The social environment, the layout of health service facilities, and residents’ travel habits were related to health service facility satisfaction. Results also showed significant differences in various facilities’ accessibility satisfaction between objective measurement and residents’ perception measurement. Compared with subjective measurement, the objective measurements of accessibility for sports venues (objectively measured average minus perceived average: −1.310), sports zones (−0.740), and specialized hospitals (−1.081) were lower; those for community hospitals (0.095), clinics (1.025), and pharmacies (0.765) were higher; and facility accessibility measured by subjective perception had a more significant impact on health facility satisfaction. Pharmacies (OR: 1.932) and community hospitals (OR: 1.751) had the largest impact among the eight types of facilities. Conclusion: This study proposed to construct a healthy community-life circle with a category and hierarchy system.
Arbuscular Mycorrhizal Fungi and Glomalin Play a Crucial Role in Soil Aggregate Stability in Pb-Contaminated Soil
Authors: Li Yinong , Xu Jiazheng , Hu Jin , Zhang Tianyu , Wu Xuefeng , Yang Yurong
Published: April 2022

With the rapid development of industrialization and urbanization, soil contamination with heavy metal (HM) has gradually become a global environmental problem. Lead (Pb) is one of the most abundant toxic metals in soil and high concentrations of Pb can inhibit plant growth, harm human health, and damage soil properties, including quality and stability. Arbuscular mycorrhizal fungi (AMF) are a type of obligate symbiotic soil microorganism forming symbiotic associations with most terrestrial plants, which play an essential role in the remediation of HM-polluted soils. In this study, we investigated the effects of AMF on the stability of soil aggregates under Pb stress in a pot experiment. The results showed that the hyphal density (HLD) and spore density (SPD) of the AMF in the soil were significantly reduced at Pb stress levels of 1000 mg kg−1 and 2000 mg kg−1. AMF inoculation strongly improved the concentration of glomalin-related soil protein (GRSP). The percentage of soil particles >2 mm and 2–1 mm in the AMF-inoculation treatment was higher than that in the non-AMF-inoculation treatment, while the Pb stress increased the percentage of soil particles <0.053 mm and 0.25–0.53 mm. HLD, total glomalin-related soil protein (T-GRSP), and easily extractable glomalin-related soil protein (EE-GRSP) were the three dominant factors regulating the stability of the soil aggregates, based on the random forest model analysis. Furthermore, the structural equation modeling analysis indicated that the Pb stress exerted an indirect effect on the soil-aggregate stability by regulating the HLD or the GRSP, while only the GRSP had a direct effect on the mean weight diameter (MWD) and geometric mean diameter (GMD). The current study increases the understanding of the mechanism through which soil degradation is caused by Pb stress, and emphasizes the crucial importance of glomalin in maintaining the soil-aggregate stability in HM-contaminated ecosystems.
Methodological Investigation of Time Perspective Scoring and Quality of Life among Individuals with Multiple Sclerosis
Authors: Carl Ellen , Shevorykin Alina , Liskiewicz Amylynn
Published: April 2022

Achieving and maintaining a high quality of life following the diagnosis of chronic illness has a positive impact on the experience of illness, including delayed disease progression and fewer relapses. Time perspective has shown promising relationships with quality of life, though studies using the construct in samples with chronic illness are sparse and methodologically heterogeneous. Participants (n = 123) were diagnosed with relapsing-remitting multiple sclerosis at least five years prior to enrollment and were beginning a new disease modifying therapy (DMT). The Zimbardo Time Perspective Inventory (ZTPI) and the World Health Organization Quality of Life (WHOQoL-100) assessment were administered at baseline and the WHOQoL-100 was administered six-weeks after starting the new DMT. This study investigated the utility of three common methods of scoring and interpreting ZTPI (balanced vs. deviation-from-balanced, categorical, and continuous scores) to predict change in quality of life. Independent sample t-tests revealed no difference in quality of life for balanced vs. deviation-from-balanced. One-way ANOVA revealed no difference in quality of life across time perspective categories. Linear regression analysis found that past-negative scores predicted decreases in all quality of life domains as well as overall score while present hedonistic scores predicted increases in psychological and overall quality of life.
How Do Urban Land Expansion, Land Finance, and Economic Growth Interact?
Authors: Zhao Ke , Chen Danling , Zhang Xupeng , Zhang Xiaojie
Published: April 2022

Land finance has consumed a lot of China’s urban land resources while contributing to its economic growth. Urban land expansion, land finance, and economic growth have attracted significant scholarly and social attention. However, the influence mechanisms among them have not yet been fully investigated. Based on a conceptual framework analysis, in this study, the panel unit-root test, system-GMM, panel Granger causality test, impulse-response analysis, and variance decomposition were used to analyze the interactional relationships among urban land expansion, land finance, and economic growth for 30 provinces in mainland China during the period of 2000–2017. The findings show that these three factors interact with each other. Land finance exhibits a positive effect on urban land expansion and economic growth. This result is further supported by the Granger causality tests. Moreover, the VAR Granger causality-test results show a unidirectional causality flowing from urban land expansion to economic growth. The impulse-response analysis also reveals that the responses of urban land expansion to shocks in land finance appear to be positive throughout the 10 periods, which is similar to the reaction of economic growth to shocks in land finance. The result of variance decomposition indicates that the explanatory power of urban land expansion for land finance increased from 0.20% to 1.90%. In contrast, the changes in economic growth made the lowest contributions to urban land expansion and land finance. The latter made the highest contribution to economic growth, with average contribution rate of 65.26%. The findings of this study provide valuable policy implications for China, heading for a high-quality development stage.
Three-Dimensional CBCT Based Evaluation of the Maxillary Sinus by Facial Index
Authors: Lee Jeong-Hyun , Park Jong-Tae
Published: April 2022

The maxillary sinus growth is initiated 3 months after birth, and it grows lateral and inferior until the pneumatization of the alveolar bone occurs. The facial skeleton has recently been determined as affecting the maxillary sinus, prompting additional studies on changes in the size of the maxillary sinus. This study aimed to determine the size of the maxillary sinus using a 3D program after categorizing South Korean adults according to their facial index (FI) classification. The participants of this study were 60 patients in their 20s, who visited the orthodontic department of Dankook University Dental Hospital (approval no. DUDH IRB 2015-12-022). The CBCT of the patients were extracted and measured as 3D images using Mimics (version 22.0, Materialise, Leuven, Belgium). Upon categorizing the subjects based on their FI classification, they were grouped into the mesoprosopic, leptoprosopic, and hyperleptoprosopic types. A one-way ANOVA was performed to evaluate the mean differences of the maxillary sinus, depending on the FI classification. In this study, the maxillary sinus tended to be wider in those with mesoprosopic type, and tended to be higher in the hyperleptoprosopic type, suggesting a need for clinicians to focus to the shape of the face during clinical treatments.
Evaluating the Impact of Metro Interior Noise on Passenger Annoyance: An Experimental Study
Authors: Ma Meng , Ran Wenqian , Wu Jinping , Li Minghang , Qu Xiangyu
Published: April 2022

The operation of a large-scale metro system creates problematic interior noise; the impact of this noise on passengers and drivers is a subject of increasing concern. To investigate the quantitative relationship between metro interior noise and passengers’ annoyance, this study analyzed questionnaires on passenger annoyance completed by 118 volunteers. The feedback from the questionnaire concerned eleven metro lines in Beijing. To test the interior noise levels, the volunteers were divided into two groups: A and B. The volunteers in group A took the same metro train as the testers, whereas those in group B took different trains. A total of 2080 noise annoyance samples from metro tunnel sections were collected and analyzed. Finally, the exposure-response relationship between interior noise and passenger annoyance was obtained by fitting these data with a logistic function. The results indicated that there was a significant positive correlation between the average subjective annoyance and the averaged equivalent sound pressure level. The fitting result was better for group A than for group B. For the mixed samples of two groups, the fitting result was greatly affected by the contribution of group A. To provide an acoustically comfortable environment, metro interior noise should not exceed 84–85 dB(A).
TRPA1 Role in Inflammatory Disorders: What Is Known So Far?
Authors: Landini Lorenzo , Souza Monteiro de Araujo Daniel , Titiz Mustafa , Geppetti Pierangelo , Nassini Romina , De Logu Francesco
Published: April 2022

The transient receptor potential ankyrin 1 (TRPA1), a member of the TRP superfamily of channels, is primarily localized in a subpopulation of primary sensory neurons of the trigeminal, vagal, and dorsal root ganglia, where its activation mediates neurogenic inflammatory responses. TRPA1 expression in resident tissue cells, inflammatory, and immune cells, through the indirect modulation of a large series of intracellular pathways, orchestrates a range of cellular processes, such as cytokine production, cell differentiation, and cytotoxicity. Therefore, the TRPA1 pathway has been proposed as a protective mechanism to detect and respond to harmful agents in various pathological conditions, including several inflammatory diseases. Specific attention has been paid to TRPA1 contribution to the transition of inflammation and immune responses from an early defensive response to a chronic pathological condition. In this view, TRPA1 antagonists may be regarded as beneficial tools for the treatment of inflammatory conditions.
Metabolomic Analysis of Serum and Tear Samples from Patients with Obesity and Type 2 Diabetes Mellitus
Authors: Nokhoijav Erdenetsetseg , Guba Andrea , Kumar Ajneesh , Kunkli Balázs , Kalló Gergő , Káplár Miklós , Somodi Sándor , Garai Ildikó , Csutak Adrienne , Tóth Noémi , Emri Miklós , Tőzsér József , Csősz Éva
Published: April 2022

Metabolomics strategies are widely used to examine obesity and type 2 diabetes (T2D). Patients with obesity (n = 31) or T2D (n = 26) and sex- and age-matched controls (n = 28) were recruited, and serum and tear samples were collected. The concentration of 23 amino acids and 10 biogenic amines in serum and tear samples was analyzed. Statistical analysis and Pearson correlation analysis along with network analysis were carried out. Compared to controls, changes in the level of 6 analytes in the obese group and of 10 analytes in the T2D group were statistically significant. For obesity, the energy generation, while for T2D, the involvement of NO synthesis and its relation to insulin signaling and inflammation, were characteristic. We found that BCAA and glutamine metabolism, urea cycle, and beta-oxidation make up crucial parts of the metabolic changes in T2D. According to our data, the retromer-mediated retrograde transport, the ethanolamine metabolism, and, consequently, the endocannabinoid signaling and phospholipid metabolism were characteristic of both conditions and can be relevant pathways to understanding and treating insulin resistance. By providing potential therapeutic targets and new starting points for mechanistic studies, our results emphasize the importance of complex data analysis procedures to better understand the pathomechanism of obesity and diabetes.
Temporal Bone Squamous Cell Carcinoma: Molecular Markers Involved in Carcinogenesis, Behavior, and Prognosis: A Systematic Review
Authors: Alessandrini Lara , Astolfi Laura , Franz Leonardo , Gentilin Erica , Mazzoni Antonio , Zanoletti Elisabetta , Marioni Gino
Published: April 2022

Temporal bone squamous cell carcinoma (TBSCC) is an uncommon malignancy with a poor prognosis in advanced cases. The dismal outcome of advanced TBSSC cases is largely due to the cancer’s local aggressiveness and the complex anatomy of this region, as well as to persistent pitfalls in diagnosis and treatment. Molecular changes occur in malignancies before any morphological changes become visible, and are responsible for the disease’s clinical behavior. The main purpose of this critical systematic review is to assess the level of knowledge on the molecular markers involved in the biology, behavior, and prognosis of TBSCC. A search (updated to March 2022) was run in PubMed, Scopus, and Web of Science electronic databases without publication date limits for studies investigating molecular markers in cohorts of patients with primary TBSCC. The search terms used were: “temporal bone” OR “external auditory canal” OR “ear”, AND “cancer” OR “carcinoma” OR “malignancy”. We preliminarily decided not to consider series with less than five cases. Twenty-four case series of TBSCC were found in which different analytical techniques had been used to study the role of several biomarkers. In conclusion, only very limited information on the prognostic role of molecular markers in TBSCC are currently available; prospective, multi-institutional, international prognostic studies should be planned to identify the molecular markers involved in the clinical behavior and prognosis of TBSCC. A further, more ambitious goal would be to find targets for therapeutic agents able to improve disease-specific survival in patients with advanced TBSCC.
Integrated Degradome and Srna Sequencing Revealed miRNA-mRNA Regulatory Networks between the Phloem and Developing Xylem of Poplar
Authors: Ding Changjun , Shen Tengfei , Ran Na , Zhang Heng , Pan Huixin , Su Xiaohua , Xu Meng
Published: April 2022

Lignin and cellulose are the most abundant natural organic polymers in nature. MiRNAs are a class of regulatory RNAs discovered in mammals, plants, viruses, and bacteria. Studies have shown that miRNAs play a role in lignin and cellulose biosynthesis by targeting key enzymes. However, the specific miRNAs functioning in the phloem and developing xylem of Populus deltoides are still unknown. In this study, a total of 134 miRNAs were identified via high-throughput small RNA sequencing, including 132 known and two novel miRNAs, six of which were only expressed in the phloem. A total of 58 differentially expressed miRNAs (DEmiRNAs) were identified between the developing xylem and the phloem. Among these miRNAs, 21 were significantly upregulated in the developing xylem in contrast to the phloem and 37 were significantly downregulated. A total of 2431 target genes of 134 miRNAs were obtained via high-throughput degradome sequencing. Most target genes of these miRNAs were transcription factors, including AP2, ARF, bHLH, bZIP, GRAS, GRF, MYB, NAC, TCP, and WRKY genes. Furthermore, 13 and nine miRNAs were involved in lignin and cellulose biosynthesis, respectively, and we validated the miRNAs via qRT-PCR. Our study explores these miRNAs and their regulatory networks in the phloem and developing xylem of P.deltoides and provides new insight into wood formation.
A Bioengineered In Vitro Model to Assess AAV-Based Gene Therapies for Cyclic GMP-Related Disorders
Authors: Pavlou Marina , Babutzka Sabrina , Michalakis Stylianos
Published: April 2022

The emergence of efficient viral vectors derived from adeno-associated viruses (AAV) has led many groups to develop gene therapies for inherited monogenic diseases, such as retinal dystrophies. To evaluate the potency of new gene therapy vectors in a preclinical context, it is common to use animal models, such as gene-deficient or mutant animal models of a given human disease, and then assess vision restoration with functional or behavioral assays. While such animal models are invaluable to the preclinical testing process, they cannot be readily used as batch release tests during manufacturing or to validate biological activity at later stages of development. There is therefore a need for rapid and reliable in vitro models that can determine whether therapeutic vectors have delivered their cargo gene, and more importantly, whether this has resulted in the intended biological activity. Given our previous experience, we chose CNGA3-linked achromatopsia to develop a cell-based system to verify biological activity of AAV vectors designed to deliver a healthy CNGA3 gene copy into human cone photoreceptors. Our system is based on an immortalized cell line with high susceptibility to AAV transduction, i.e., HeLa cells, which we engineered to express a fungal rhodopsin guanylyl cyclase (RhGC) from Blastocladiella emersonii and a sensitive genetically encoded calcium indicator (GECI) under the control of a tetracycline operator. Using this system, we were able to confirm and quantify the function of the ion channel encoded by AAV/CNGA3 and differentiate between AAV vector potencies with a simple fluorometric assay. Finally, we show that this approach can be readily adapted for the assessment of phosphodiesterase function.
Influence of Cr Ion Implantation on Physical Properties of CuO Thin Films
Authors: Ungeheuer Katarzyna , Marszalek Konstanty Waldemar , Mitura-Nowak Marzena , Perzanowski Marcin , Jelen Piotr , Marszalek Marta , Sitarz Maciej
Published: April 2022

Cupric oxide is a semiconductor with applications in sensors, solar cells, and solar thermal absorbers. To improve its properties, the oxide was doped with a metallic element. No studies were previously performed on Cr-doping using the ion implantation technique. The research goal of these studies is to investigate how Cr ion implantation impacts the properties of the oxide thin films. CuO thin films were deposited using magnetron sputtering, and then chromium ions with different energies and doses were implanted. Structural, optical, and vibrational properties of the samples were studied using X-ray diffraction, X-ray reflectivity, infra-red spectroscopy, Raman spectroscopy, and spectrophotometry. The surface morphology and topography were studied with ellipsometry, atomic force microscopy, and scanning electron microscopy. A simulation of the range of ions in the materials was performed. Ion implantation had an impact on the properties of thin films that could be used to tailor the optical properties of the cupric oxide and possibly also its electrical properties. A study considering the influence of ion implantation on electrical properties is proposed as further research on ion-implanted CuO thin films.
The Pathogenesis of Giant Condyloma Acuminatum (Buschke-Lowenstein Tumor): An Overview
Authors: Purzycka-Bohdan Dorota , Nowicki Roman J. , Herms Florian , Casanova Jean-Laurent , Fouéré Sébastien , Béziat Vivien
Published: April 2022

Giant condyloma acuminatum, also known as Buschke-Lowenstein tumor (BLT), is a rare disease of the anogenital region. BLT is considered a locally aggressive tumor of benign histological appearance, but with the potential for destructive growth and high recurrence rates. BLT development is strongly associated with infection with low-risk human papillomaviruses (HPVs), mostly HPV-6 and -11. Immunity to HPVs plays a crucial role in the natural control of various HPV-induced lesions. Large condyloma acuminata are frequently reported in patients with primary (e.g., DOCK8 or SPINK5 deficiencies) and secondary (e.g., AIDS, solid organ transplantation) immune defects. Individuals with extensive anogenital warts, including BLT in particular, should therefore be tested for inherited or acquired immunodeficiency. Research into the genetic basis of unexplained cases is warranted. An understanding of the etiology of BLT would lead to improvements in its management. This review focuses on the role of underlying HPV infections, and human genetic and immunological determinants of BLT.
Physiological and Pathological Significance of Esophageal TRP Channels: Special Focus on TRPV4 in Esophageal Epithelial Cells
Authors: Boudaka Ammar , Tominaga Makoto
Published: April 2022

Transient receptor potential vanilloid 4 (TRPV4) is a non-selective cation channel that is broadly expressed in different human tissues, including the digestive system, where it acts as a molecular sensor and a transducer that regulates a variety of functional activities. Despite the extensive research to determine the role of this channel in the physiology and pathophysiology of different organs, the unique morphological and functional features of TRPV4 in the esophagus remain largely unknown. Ten years ago, TRPV4 was shown to be highly expressed in esophageal epithelial cells where its activation induces Ca2+-dependent ATP release, which, in turn, mediates several functions, ranging from mechanosensation to wound healing. This review summarizes the research progress on TRPV4, and focuses on the functional expression of TRPV4 in esophageal epithelium and its possible role in different esophageal diseases that would support TRPV4 as a candidate target for future therapeutic approaches to treat patients with these conditions.
Novel Strategies for Spinal Cord Regeneration
Authors: Costăchescu Bogdan , Niculescu Adelina-Gabriela , Dabija Marius Gabriel , Teleanu Raluca Ioana , Grumezescu Alexandru Mihai , Eva Lucian
Published: April 2022

A spinal cord injury (SCI) is one of the most devastating lesions, as it can damage the continuity and conductivity of the central nervous system, resulting in complex pathophysiology. Encouraged by the advances in nanotechnology, stem cell biology, and materials science, researchers have proposed various interdisciplinary approaches for spinal cord regeneration. In this respect, the present review aims to explore the most recent developments in SCI treatment and spinal cord repair. Specifically, it briefly describes the characteristics of SCIs, followed by an extensive discussion on newly developed nanocarriers (e.g., metal-based, polymer-based, liposomes) for spinal cord delivery, relevant biomolecules (e.g., growth factors, exosomes) for SCI treatment, innovative cell therapies, and novel natural and synthetic biomaterial scaffolds for spinal cord regeneration.
Tracking Changes in the Spring Barley Gene Pool in Poland during 120 Years of Breeding
Authors: Dziurdziak Joanna , Podyma Wiesław , Bujak Henryk , Boczkowska Maja
Published: April 2022

This study was undertaken to investigate the diversity and population structure of 83 spring barley (Hordeum vulgare L.) cultivars, which corresponded to 120 years of this crop’s breeding in Poland. The analysis was based on 11,655 DArTseq-derived SNPs evenly distributed across seven barley chromosomes. Five groups were assigned in the studied cultivars according to the period of their breeding. A decrease in observed heterozygosity within the groups was noted along with the progress in breeding, with a simultaneous increase in the inbreeding coefficient value. As a result of breeding, some of the unique allelic variation present in old cultivars was lost, but crosses with foreign materials also provided new alleles to the barley gene pool. It is important to mention that the above changes affected different chromosomes to varying degrees. The internal variability of the cultivars ranged from 0.011 to 0.236. Internal uniformity was lowest among the oldest cultivars, although some highly homogeneous ones were found among them. This is probably an effect of genetic drift or selection during their multiplications and regenerations in the period from breeding to the time of analysis. The population genetic structure of the studied group of cultivars appears to be quite complex. It was shown that their genetic makeup consists of as many as eleven distinct gene pools. The analysis also showed traces of directed selection on chromosomes 3H and 5H. Detailed data analysis confirmed the presence of duplicates for 11 cultivars. The performed research will allow both improvement of the management of barley genetic resources in the gene bank and the reuse of this rich and forgotten variability in breeding programs and research.
Prognostic Significance of GPR55 mRNA Expression in Colon Cancer
Authors: Ismail Hager Tarek H. , AbdelMageed Manar , Lindmark Gudrun , Hammarström Marie-Louise , Hammarström Sten , Sitohy Basel
Published: April 2022

G protein-coupled receptor 55 (GPR55) probably plays a role in innate immunity and tumor immunosurveillance through its effect on immune cells, such as T cells and NK cells. In this study, the prognostic value of GPR55 in colon cancer (CC) was investigated. mRNA expression levels of GPR55 were determined in 382 regional lymph nodes of 121 CC patients with 12 years observation time after curative surgery. The same clinical material had previously been analyzed for expression levels of CEA, CXCL16, CXCL17, GPR35 V2/3 and LGR5 mRNAs. Clinical cutoffs of 0.1365 copies/18S rRNA unit for GPR55 and 0.1481 for the GPR55/CEA ratio were applied to differentiate between the high- and low-GPR55 expression groups. Kaplan–Meier survival analysis and Cox regression risk analysis were used to determine prognostic value. Improved discrimination between the two groups was achieved by combining GPR55 with CEA, CXCL16 or CXCL17 compared with GPR55 alone. The best result was obtained using the GPR55/CEA ratio, with an increased mean survival time of 14 and 33 months at 5 and 12 years observation time, respectively (p = 0.0003 and p = 0.003) for the high-GPR55/CEA group. The explanation for the observed improvement is most likely that GPR55 is a marker for T cells and B cells in lymph nodes, whereas CEA, CXCL16 and CXCL17, are markers for tumor cells of epithelial origin.
Metabolic Activation and DNA Interactions of Carcinogenic N-Nitrosamines to Which Humans Are Commonly Exposed
Authors: Li Yupeng , Hecht Stephen S.
Published: April 2022

Carcinogenic N-nitrosamine contamination in certain drugs has recently caused great concern and the attention of regulatory agencies. These carcinogens—widely detectable in relatively low levels in food, water, cosmetics, and drugs—are well-established and powerful animal carcinogens. The electrophiles resulting from the cytochrome P450-mediated metabolism of N-nitrosamines can readily react with DNA and form covalent addition products (DNA adducts) that play a central role in carcinogenesis if not repaired. In this review, we aim to provide a comprehensive and updated review of progress on the metabolic activation and DNA interactions of 10 carcinogenic N-nitrosamines to which humans are commonly exposed. Certain DNA adducts such as O6-methylguanine with established miscoding properties play central roles in the cancer induction process, whereas others have been linked to the high incidence of certain types of cancers. We hope the data summarized here will help researchers gain a better understanding of the bioactivation and DNA interactions of these 10 carcinogenic N-nitrosamines and facilitate further research on their toxicologic and carcinogenic properties.