bluuebunny/crossref_metadata_2025 huggingface dataset:
Additively Manufactured Porous Ti6Al4V for Bone Implants: A Review
Authors: Koju Naresh , Niraula Suyash , Fotovvati Behzad
Published: April 2022

Ti-6Al-4V (Ti64) alloy is one of the most widely used orthopedic implant materials due to its mechanical properties, corrosion resistance, and biocompatibility nature. Porous Ti64 structures are gaining more research interest as bone implants as they can help in reducing the stress-shielding effect when compared to their solid counterpart. The literature shows that porous Ti64 implants fabricated using different additive manufacturing (AM) process routes, such as laser powder bed fusion (L-PBF) and electron beam melting (EBM) can be tailored to mimic the mechanical properties of natural bone. This review paper categorizes porous implant designs into non-gradient (uniform) and gradient (non-uniform) porous structures. Gradient porous design appears to be more promising for orthopedic applications due to its closeness towards natural bone morphology and improved mechanical properties. In addition, this paper outlines the details on bone structure and its properties, mechanical properties, fatigue behavior, multifunctional porous implant designs, current challenges, and literature gaps in the research studies on porous Ti64 bone implants.
Corrosion Behavior of Multiphase Bainitic Rail Steels
Authors: Rojhirunsakool Tanaporn , Thublaor Thammaporn , Bidabadi Mohammad Hassan Shirani , Chandra-ambhorn Somrerk , Yang Zhigang , Gao Guhui
Published: April 2022

Pearlitic steel experiences excessive corrosion in a hot and humid atmosphere. The multiphase bainitic/martensitic structure was developed for a better combination of strength and ductility, especially rolling contact fatigue, but little attention to corrosion has been investigated. Corrosion behaviors of multiphase steels obtained from bainitic-austempering (BAT) and bainitic-quenching and -partitioning (BQ&P) processes were investigated via immersion and electrochemical tests in 3.5 wt.% NaCl solution. The corroded surface and rust after immersion and electrochemical tests were analyzed via electron microscopy, Fourier transform infrared spectra, and x-ray diffraction. The multiphase bainite + martensite/retained austenite island showed higher corrosion resistance than that of the pearlitic one. The acicular bainite obtained from the BQ&P process showed slightly higher corrosion resistance than the granular bainite + martensite structure obtained from the BAT process.
Weld Formation, Microstructure Evolution and Mechanical Property of Laser-Arc Hybrid Welded AZ31B Magnesium Alloy
Authors: Wang Sibo , Yan Chunyan , Gu Zhengjia
Published: April 2022

Laser-arc hybrid welding of AZ31B magnesium alloy was carried out in this paper, and the effects of welding parameters (laser power, welding speed, welding current) on weld formation, microstructure evolution and mechanical property were studied. The results showed that laser-arc hybrid welding can inhibit the undercut defect during laser welding on the one hand, and enhance the arc stability on the other hand. The penetration depth and width showed no significant relation with the total heat input, but were linear related to the laser heat input and the arc heat input, respectively. The average grain size of the equiaxed grains was closely related to the heat input. The higher the heat input was, the larger the grains. The optimized welding parameters were laser power of 3.5 kW, welding speed of 1.8 m/min and welding current of 100 A. In this case, the weld was free of undercut and pores, and the tensile strength and elongation rate reached 190 MPa and 12%, respectively.
Analytical Model for Temperature Prediction in Milling AISI D2 with Minimum Quantity Lubrication
Authors: Cai Linger , Feng Yixuan , Lu Yu-Ting , Lin Yu-Fu , Hung Tsung-Pin , Hsu Fu-Chuan , Liang Steven Y.
Published: April 2022

Milling with minimum quantity lubrication (MQL) is now a commonly used machining technique in industry. The application of the MQL significantly reduces the temperature on the machined surface, while the cost of the lubricants is limited and the pollution caused by the lubricants is better controlled. However, the fast prediction of the milling temperature during the process has not been well developed. This paper proposes an analytical model for milling temperature prediction at the workpiece flank surface with MQL application. Based on the modified orthogonal cutting model and boundary layer lubrication effect, the proposed model takes in the process parameters and can generate the temperature profile at the workpiece surface within 1 min. The model is validated with experimental data in milling AISI D2 steel. With an average absolute error of 10.38%, the proposed model provides a reasonable temperature prediction compared to the experimental results. Based on the proposed model, this paper also investigates the effect of different cutting parameters on the cutting temperature. It is found that the application of the MQL decreases the temperature at the cutting zone, especially at the flank surface of the workpiece, which is due to the heat loss led by air-oil flow.
Analysis of Metabolomic Changes in Xylem and Phloem Sap of Cucumber under Phosphorus Stresses
Authors: Sun Jingjing , Li Qinglin , Xu Hui , Zhang Wentao
Published: April 2022

Cucumber xylem and phloem sap is a key link in nutrient distribution, transportation and signal transduction of cucumber plants; however, the metabolic response mechanism of cucumber xylem and phloem sap under phosphorus stress has not been clearly revealed. In this study, gas chromatography-mass spectrometry (GC-MS) combined with principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were used to analyze the metabolites in cucumber xylem and phloem sap under different phosphorus stress. A total of 22 differential metabolites were screened from xylem and phloem sap, respectively. Through the analysis of metabolic pathways of differential metabolites, four and three key metabolic pathways were screened, respectively. The results showed that compared with the normal phosphorus level, the content of most amino acids in the key metabolic pathway increased in xylem but decreased in phloem both under low and high phosphorus stress levels. The contents of sucrose and glucose in phloem glycolysis pathway showed a positive correlation with the change of phosphorus nutrient levels. The tricarboxylic acid cycle was promoted in xylem and phloem of cucumber under low and high phosphorus nutrient levels, and the contents of malic acid and citric acid increased significantly. This study provided abundant biochemical information for the metabolic response and regulation strategies of cucumber xylem and phloem under phosphorus stress, and is committed to looking for more sensitive markers to evaluate the supply level of phosphorus nutrients in cucumber.
Early Effects of Metabolic Syndrome on ATP-Sensitive Potassium Channels from Rat Pancreatic Beta Cells
Authors: Cruz-Cruz Iskra , Bernate-Obando Germán , Larqué Carlos , Escalona Rene , Pinto-Almazán Rodolfo , Velasco Myrian
Published: April 2022

Metabolic syndrome (MS) is a cluster of metabolic signs that increases the risk of developing type 2 two diabetes mellitus and cardiovascular diseases. MS leads to pancreatic beta cell exhaustion and decreased insulin secretion through unknown mechanisms in a time-dependent manner. ATP-sensitive potassium channels (KATP channels), common targets of anti-diabetic drugs, participate in the glucose-stimulated insulin secretion, coupling the metabolic status and electrical activity of pancreatic beta cells. We investigated the early effects of MS on the conductance, ATP and glybenclamide sensitivity of the KATP channels. We used Wistar rats fed with a high-sucrose diet (HSD) for 8 weeks as a MS model. In excised membrane patches, control and HSD channels showed similar unitary conductance and ATP sensitivity pancreatic beta cells in their KATP channels. In contrast, MS produced variability in the sensitivity to glybenclamide of KATP channels. We observed two subpopulations of pancreatic beta cells, one with similar (Gly1) and one with increased (Gly2) glybenclamide sensitivity compared to the control group. This study shows that the early effects of MS produced by consuming high-sugar beverages can affect the pharmacological properties of KATP channels to one of the drugs used for diabetes treatment.
The Effect of Ink Supply Pressure on Piezoelectric Inkjet
Authors: Kim San , Choi Jun Hyeok , Sohn Dong Kee , Ko Han Seo
Published: April 2022

Experimental and numerical analysis of the drop-on-demand inkjet was conducted to determine the jetting characteristics and meniscus motion under the control of the ink supply pressure. A single transparent nozzle inkjet head driven by a piezoelectric actuator was used to eject droplets. To control ink supply pressure, the pressure of the air in the reservoir was regulated by a dual valve pressure controller. The inkjet performance and the motion of the meniscus were evaluated by visualization and numerical simulation. A two-dimensional axisymmetric numerical simulation with the dynamic mesh method was performed to simulate the inkjet dynamics, including the actual deformation of the piezoelectric actuator. Numerical simulation showed good agreement with the experimental results of droplet velocity and volume with an accuracy of 87.1%. Both the experimental and simulation results showed that the drop volume and velocity were linearly proportional to the voltage change. For the specific voltages, an analysis of the effect of the ink supply pressure control was conducted. At the maximum negative pressure, −3 kPa, the average velocity reductions were 0.558 and 0.392 m/s in the experiment and simulation, respectively, which were 18.7 and 11.6% less than those of the uncontrolled case of 0 kPa. Therefore, the simulation environment capable of simulating the entire inkjet dynamics, including meniscus movement regarded to be successfully established. The average volume reductions were 18.7 and 6.97 pL for the experiment and simulation, respectively, which were 21.7 and 9.17% less than those of the uncontrolled case. In the results of the meniscus motion simulation, the damping of the residual vibration agreed well with the experimental results according to the ink supply pressure change. Reducing the ink supply pressure reduced the speed and volume, improved the damping of residual vibrations, and suppressed satellite drops. Decreasing ink supply pressure can be expected to improve the stability and productivity of inkjet printing.
Refined Simulation Method for Computer-Aided Process Planning Based on Digital Twin Technology
Authors: Xin Yupeng , Chen Yiwen , Li Wenhui , Li Xiuhong , Wu Fengfeng
Published: April 2022

Simulation technology is widely used in computer-aided process planning (CAPP). The part machining process is simulated in the virtual world, which can predict manufacturing errors and optimize the process plan. Simulation accuracy is the guarantee of process decision-making and optimization. This article focuses on the use of digital twin technology to build a high-fidelity process model, taking the advantage of the integration of multiple systems, in order to achieve the dynamic association of real-time manufacturing data and process models. Making use of the CAPP/MES systems, the surface inspection data of the part is fed back to the CAPP system and associated with the digital twin process model. The wavelet transform method is used to reduce the noise of the high-frequency signal of the detection data, and the signal-to-noise ratio (SNR) is calculated to verify the noise reduction effect. The surface topography, after noise reduction, was reconstructed in Matlab. On this basis, the Poisson reconstruction algorithm is used to reconstruct the high-fidelity process model for the refined simulation of the subsequent processes. Finally, by comparing the two sets of simulation experiments with the real machining results, we found that the simulation results, based on the digital twin model, are more accurate than the traditional simulation method by 58%.
Thermal Management of Serpentine Flexible Heater Based on the Orthotropic Heat Conduction Model
Authors: Zhao Zhao , Nan Jin , Li Min
Published: April 2022

Flexible heaters can perfectly fit with undevelopable surfaces for heating in many practical applications such as thermotherapy, defogging/deicing systems and warming garments. Considering the requirement for stretchability in a flexible heater, certain spacing needs to be retained between serpentine heat sources for deformation which will inevitably bring critical challenges to the thermal uniformity. In order to reconcile these two conflicting aspects, a novel method is proposed by embedding the serpentine heat source in orthotropic layers to achieve comprehensive performance in stretchability and uniform heating. Such a scheme takes advantage of the ability of orthotropic material to control the heat flow distribution via orthotropic thermal conductivity. In this paper, an analytical heat conduction model with orthotropic substrate and encapsulation is calculated using Fourier cosine transform, which is validated by finite element analysis (FEA). Meanwhile, the effects of the orthotropic substrate or encapsulation with different ratios of thermal conductivity and the geometric spacing on the thermal properties are investigated, which can help guide the design and fabrication of flexible heaters to achieve the goal of uniform heating.
Energy Harvester Based on an Eccentric Pendulum and Wiegand Wires
Authors: Chen Yi-Hsin , Lee Chien , Wang Yu-Jen , Chang You-Yu , Chen Yi-Cheng
Published: April 2022

This study proposed an energy harvester that combines an eccentric pendulum with Wiegand wires to harvest the kinetic energy of a rotating plate. The energy harvester converts the kinetic energy into electrical energy to power sensors mounted on the rotating plate or wheel. The kinetic model is derived from the Euler–Lagrange equation. The eccentric pendulum generates a swing motion from the direction variation of the centrifugal force and the gravitational force. The magnetic circuit is designed such that, during the swing motion, an alternating magnetic field is formed to induce the output voltage of the Wiegand wire. COMSOL software was used to simulate magnetic flux density and optimize the geometric parameters of magnets. Response surface methodology was used to formulate the output voltage model. Magnetic flux density affects output voltage dramatically. However, the output voltage is not sensitive to the gradient of magnetic flux density. The experimental results indicate that when the Wiegand wire is 14.2 mm from the magnet, the generation power is 0.118–1.15 mW, in a speed range of 240–540 rpm. When the Wiegand wire is 7.0 mm from the magnet, the generation power is 0.741–1.06 mW, in a speed range of 480–660 rpm.
Static High Voltage Actuation of Piezoelectric AlN and AlScN Based Scanning Micromirrors
Authors: Stoeckel Chris , Meinel Katja , Melzer Marcel , Žukauskaitė Agnė , Zimmermann Sven , Forke Roman , Hiller Karla , Kuhn Harald
Published: April 2022

Piezoelectric micromirrors with aluminum nitride (AlN) and aluminum scandium nitride (Al0.68Sc0.32N) are presented and compared regarding their static deflection. Two chip designs with 2 × 3 mm2 (Design 1) and 4 × 6 mm2 (Design 2) footprint with 600 nm AlN or 2000 nm Al0.68Sc0.32N as piezoelectric transducer material are investigated. The chip with Design 1 and Al0.68Sc0.32N has a resonance frequency of 1.8 kHz and a static scan angle of 38.4° at 400 V DC was measured. Design 2 has its resonance at 2.1 kHz. The maximum static scan angle is 55.6° at 220 V DC, which is the maximum deflection measurable with the experimental setup. The static deflection per electric field is increased by a factor of 10, due to the optimization of the design and the research and development of high-performance piezoelectric transducer materials with large piezoelectric coefficient and high electrical breakthrough voltage.
Optical Fiber–Based Continuous Liquid Level Sensor Based on Rayleigh Backscattering
Authors: Chi Xingqiang , Wang Xiangjun , Ke Xuan
Published: April 2022

This work reports an optical fiber–based continuous liquid level sensor for cryogenic propellant mass gauging, which has significant advantages over the existing liquid level sensors in terms of accuracy, simplicity, and reliability. Based on Rayleigh backscattering coherent optical frequency domain reflectometry, every point of the sensing fiber is a liquid sensor which is able to distinguish liquid and vapor. We obtained a measurement accuracy of 1 mm for the optical fiber sensor by measuring both liquid nitrogen and water levels. For the first time, for practical applications, we experimentally studied the influence of ambient temperature and strain changes on the sensing performance as well as the repeatability of the optical fiber–based liquid level sensor’s measurements.
Conventional and Microfluidic Methods for the Detection of Nucleic Acid of SARS-CoV-2
Authors: Song Weidu , Zhang Taiyi , Lin Huichao , Yang Yujing , Zhao Gaozhen , Huang Xiaowen
Published: April 2022

Nucleic acid testing (NAT) played a crucial role in containing the spread of SARS-CoV-2 during the epidemic. The gold standard technique, the quantitative real-time polymerase chain reaction (qRT-PCR) technique, is currently used by the government and medical boards to detect SARS-CoV-2. Due to the limitations of this technology, it is not capable of meeting the needs of large-scale rapid detection. To solve this problem, many new techniques for detecting nucleic acids of SARS-CoV-2 have been reported. Therefore, a review that systematically and comprehensively introduces and compares various detection technologies is needed. In this paper, we not only review the traditional NAT but also provide an overview of microfluidic-based NAT technologies and summarize and discuss the characteristics and development prospects of these techniques.
Tunable Electromechanical Coupling Coefficient of a Laterally Excited Bulk Wave Resonator with Composite Piezoelectric Film
Authors: Xie Ying , Liu Yan , Liu Jieyu , Wang Lei , Liu Wenjuan , Soon Bo , Cai Yao , Sun Chengliang
Published: April 2022

A resonator with an appropriate electromechanical coupling coefficient (Kt2) is crucial for filter applications in radio communication. In this paper, we present an effective method to tune the Kt2 of resonators by introducing different materials into a lithium niobate (LiNbO3) piezoelectric matrix. The effective piezoelectric coefficients e33eff and e15eff of composite materials with four different introduced materials were calculated. The results show that the e15eff of SiO2/LiNbO3 composite piezoelectric material was mostly sensitive to an increase in the width of introduced SiO2 material. Simultaneously, the simulation of a laterally excited bulk wave resonator (XBAR) with SiO2/LiNbO3 composite material was also carried out to verify the change in the Kt2 originating from the variation in e15eff. The achievable n79 filter using the SiO2/LiNbO3 composite material demonstrates the promising prospects of tuning Kt2 by introducing different materials into a LiNbO3 piezoelectric matrix.
The Impacts of Lactiplantibacillus plantarum on the Functional Properties of Fermented Foods: A Review of Current Knowledge
Authors: Yilmaz Birsen , Bangar Sneh Punia , Echegaray Noemi , Suri Shweta , Tomasevic Igor , Manuel Lorenzo Jose , Melekoglu Ebru , Rocha João Miguel , Ozogul Fatih
Published: April 2022

One of the most varied species of lactic acid bacteria is Lactiplantibacillus plantarum (Lb. plantarum), formerly known as Lactobacillus plantarum. It is one of the most common species of bacteria found in foods, probiotics, dairy products, and beverages. Studies related to genomic mapping and gene locations of Lb. plantarum have shown the novel findings of its new strains along with their non-pathogenic or non-antibiotic resistance genes. Safe strains obtained with new technologies are a pioneer in the development of new probiotics and starter cultures for the food industry. However, the safety of Lb. plantarum strains and their bacteriocins should also be confirmed with in vivo studies before being employed as food additives. Many of the Lb. plantarum strains and their bacteriocins are generally safe in terms of antibiotic resistance genes. Thus, they provide a great opportunity for improving the nutritional composition, shelf life, antioxidant activity, flavour properties and antimicrobial activities in the food industry. Moreover, since some Lb. plantarum strains have the ability to reduce undesirable compounds such as aflatoxins, they have potential use in maintaining food safety and preventing food spoilage. This review emphasizes the impacts of Lb. plantarum strains on fermented foods, along with novel approaches to their genomic mapping and safety aspects.
Assessing Tick-Borne Disease Risk and Surveillance: Toward a Multi-Modal Approach to Diagnostic Positioning and Prediction
Authors: Brooks Chris , McNeely Connie L. , Maxwell Sarah P. , Thomas Kevin C.
Published: April 2022

The true extent of tick-borne disease (TBD) incidence and risk among humans is largely unknown, posing significant public health challenges. This study offers an exploratory analysis of a multimodal dataset and is part of a larger ongoing project to determine if entomological data, canine serological reports, self-reported human tick bite encounters (TBEs), and/or associated TBD diagnoses can serve as proxies for human disease risk. Focusing on the United States (U.S.), it characterizes self-reported TBD diagnoses (specifically, anaplasmosis, ehrlichiosis, and Lyme disease), co-infections, and their frequency and distribution across U.S. counties in relation to the presence of other factors related to TBD risk. Survey data was used to construct a list of TBEs localizable to individual U.S. counties. National data regarding these counties—namely the presence of official Lyme Disease (LD) case reports from the Centers for Disease Control and Prevention, as well as the tick vectors I. scapularis and I. pacificus within a given county—were then linked with survey-reported TBEs, tabulated by diagnosis (including co-infections), to determine the distribution of county-level endpoints across diagnostic categories. In addition, data on the presence of positive serological diagnostic tests conducted in canines were considered due to their potential utility as a proxy for TBD and TBE risk. The final dataset contained 249 TBEs localized to a total of 144 counties across 30 states. Diagnostic categories included respondents with LD (n = 70) and those with anaplasmosis and ehrlichiosis diagnoses and co-infections (n < 20 per diagnostic category). TBEs also were indicated by respondents who did not report TBD diagnoses, with some indicating uncertainty. The distribution of respondent-reported TBEs varied between canine TBDs, with LD-positive respondents reporting noticeably larger proportions of TBEs in counties with canine LD and smaller proportions in counties with canine anaplasmosis, compared to respondents without an LD diagnosis; a notional logistic regression suggests these differences may be significant (canine LD: Odds Ratio [OR] = 6.04, p = 0.026) (canine anaplasmosis: OR = 0.50, p = 0.095). These results suggest that certain widely available diagnostic TBD data in animals (in this case, domesticated dogs) may be sensitive to differences in human TBD risk factors and thus may have utility as proxies in future research. In the absence of an available standardized, unified, and national TBD database, such proxies, along with relevant surveys and reports, may provide a much-needed working solution for scientists and clinicians studying TBDs.
Advances in the Understanding of the Lifecycle of Photosystem II
Authors: Johnson Virginia M. , Pakrasi Himadri B.
Published: April 2022

Photosystem II is a light-driven water-plastoquinone oxidoreductase present in cyanobacteria, algae and plants. It produces molecular oxygen and protons to drive ATP synthesis, fueling life on Earth. As a multi-subunit membrane-protein-pigment complex, Photosystem II undergoes a dynamic cycle of synthesis, damage, and repair known as the Photosystem II lifecycle, to maintain a high level of photosynthetic activity at the cellular level. Cyanobacteria, oxygenic photosynthetic bacteria, are frequently used as model organisms to study oxygenic photosynthetic processes due to their ease of growth and genetic manipulation. The cyanobacterial PSII structure and function have been well-characterized, but its lifecycle is under active investigation. In this review, advances in studying the lifecycle of Photosystem II in cyanobacteria will be discussed, with a particular emphasis on new structural findings enabled by cryo-electron microscopy. These structural findings complement a rich and growing body of biochemical and molecular biology research into Photosystem II assembly and repair.
Molecular Dynamics Simulation and Cryo-Electron Microscopy Investigation of AOT Surfactant Structure at the Hydrated Mica Surface
Authors: Long Daniel M. , Greathouse Jeffery A. , Xu Guangping , Jungjohann Katherine L.
Published: April 2022

Structural properties of the anionic surfactant dioctyl sodium sulfosuccinate (AOT or Aerosol-OT) adsorbed on the mica surface were investigated by molecular dynamics simulation, including the effect of surface loading in the presence of monovalent and divalent cations. The simulations confirmed recent neutron reflectivity experiments that revealed the binding of anionic surfactant to the negatively charged surface via adsorbed cations. At low loading, cylindrical micelles formed on the surface, with sulfate head groups bound to the surface by water molecules or adsorbed cations. Cation bridging was observed in the presence of weakly hydrating monovalent cations, while sulfate groups interacted with strongly hydrating divalent cations through water bridges. The adsorbed micelle structure was confirmed experimentally with cryogenic electronic microscopy, which revealed micelles approximately 2 nm in diameter at the basal surface. At higher AOT loading, the simulations reveal adsorbed bilayers with similar surface binding mechanisms. Adsorbed micelles were slightly thicker (2.2–3.0 nm) than the corresponding bilayers (2.0–2.4 nm). Upon heating the low loading systems from 300 K to 350 K, the adsorbed micelles transformed to a more planar configuration resembling bilayers. The driving force for this transition is an increase in the number of sulfate head groups interacting directly with adsorbed cations.
The Magmatic-Hydrothermal Ore-Forming Processes of the Tonggou Cu-Zn Deposit, NW China: Constraints from Magnetite Chemistry and Fluid Inclusions
Authors: Han Chang-Cheng , Zhang Xue-Bing , Wu Shi-Shan , Liu Ying-Ting
Published: April 2022

The Tonggou deposit is a porphyry Cu and vein-type Cu-Zn mineralization system located in the Bogda Orogenic Belt, north of Eastern Tianshan. Systematic fluid inclusion analyses were performed on granular quartz from the magnetite–quartz stage and pyrite–chalcopyrite–quartz stage from the porphyry Cu mineralization. During the early stage of porphyry Cu mineralization, the ore-forming fluids were at high temperatures (450–501 °C) and high salinity (51.2–55.2 wt.% NaCl equiv.) H2O-NaCl hydrothermal fluids with fluid boiling. These fluids evolved to high temperature (412–450 °C) and intermediate to low salinity (8.3–14.2 wt.% NaCl equiv.) H2O-NaCl hydrothermal fluids during the pyrite–chalcopyrite–quartz stage. In addition, magnetite from the Tonggou deposit was studied as a marker for the ore-forming process evolution of porphyry and vein-type mineralization. Sampled magnetite can be divided into MagI (allotriomorphic magnetite from altered granodiorite), MagII (magnetite from altered granodiorite found in veinlets or as granular aggregates), MagIII (from the magnetite–quartz stage of porphyry mineralization), and MagIV (from the polymetallic sulfide–epidote–quartz stage of vein-type mineralization). Magnetite LA-ICP-MS data indicate a hydrothermal origin. The contents of Ti, Si, Al, and Ta are controlled by temperature, and these elements gradually decrease from MagI to MagIV. Moreover, fO2 has considerable influence on the substitution of Sn, V, and Mn in magnetite, and the contents of these elements generally decrease from MagI to MagIII—increasing only in MagIV. Indeed, high fO2 in the polymetallic sulfide–epidote–quartz stage (MagIV) of vein-type mineralization is shown by the presence of a replacement texture in ilmenite grains within hydrothermal magnetite. On the other hand, magnetite samples from the Tonggou deposit have relatively low Ti + V contents compared to other porphyry Cu deposits—plotting in the skarn field of the (Ti + V) vs. (Ca + Al + Mn) diagram—and shows negative correlations in the (Ti + V) vs. Sn diagram. These data indicate that the porphyry Cu mineralization at Tonggou formed at relatively lower fO2 conditions than the Tonggou vein-type mineralization and other typical porphyry Cu deposits. Finally, porphyry and vein-type mineralization at Tonggou are both sourced from the porphyry system, as a result of ore-forming fluid transfer to a different location.
Strain Energy Release and Deep Rock Failure Due to Excavation in Pre-Stressed Rock
Authors: Xiao Peng , Li Diyuan , Zhu Quanqi
Published: April 2022

Deep rock engineering is in a high pre-stressed state before excavation. In this research, a method to calculate the release of strain energy caused by excavation in pre-stressed rock is proposed. The normal stress release after excavation leads to a reduction in strain energy in rock specimens. The influence of excavation height and width on strain energy release is inconsistent under vertical loading. When the height of the hole is 1 mm, the strain energy release is large, and the increase in height of hole leads to a slow increase in the strain energy release. When the width of the hole is 1 mm, the strain energy release is very small, and the increase in the width of the hole leads to an increasingly faster release of strain energy. This strain energy release exponentially increases with the increase in the lateral pressure coefficient, showing a trend in the second power of the lateral pressure coefficient. Moreover, the tunnel failure caused by excavation under high stress is obtained by a numerical calculation. The failure modes of the deep tunnel model are strain rockbursts caused by tangential stress concentrations and spalling caused by normal stress release, which is also observed in the failure mode of the actual tunnel.
Hydrochemical Characteristics and Controlling Factors of Surface Water in Upper Nujiang River, Qinghai-Tibet Plateau
Authors: Wang Mingguo , Yang Li , Li Jingjie , Liang Qian
Published: April 2022

Rivers play an essential role in the global matter transport and hydrogeochemical cycle. Hydrogeochemical research is significant to developing and protecting water resources in the Qinghai-Tibet Plateau and its lower reaches. This study aimed to identify the hydrochemical characteristics and controlling factors of Daqu River and Gaqu River in Dingqing County, two tributaries of the upper Nujiang River. This study used spatial analysis, trilinear diagram analysis, and ion ratio analysis of hydrochemical indexes to investigate the hydrochemical characteristics and controlling factors. Results show that Ca2+ and Mg2+, and HCO3− and SO42− are the main cations and anions of these two rivers. HCO3·SO4-Mg·Ca and HCO3·SO4-Ca·Mg are the primary hydrochemical types for the surface water of Daqu and Gaqu Rivers, respectively. The influence of atmospheric precipitation and anthropogenic activities is weak. The carbonated water–rock reaction is the main Ca2+, Mg2+, and HCO3− source, and hot springs act as the primary source of SO42− and supplements Ca2+, Mg2+, and HCO3−. Mg2+ from magnesite dissolution exists in the Daqu River basin. Weak reverse cation exchange occurs in both rivers. Daqu River receives more low-mineralized glacier meltwater along the flow, whereas Gaqu River receives more high-mineralized hot spring water.
Hollow-Structure Pt-Ni Nanoparticle Electrocatalysts for Oxygen Reduction Reaction
Authors: Wang Quan , Mi Baosen , Zhou Jun , Qin Ziwei , Chen Zhuo , Wang Hongbin
Published: April 2022

An electrocatalyst with high oxygen reduction reaction (ORR) activity and high stability during start–stop operation is necessary. In this paper, hollow-structure Pt-Ni electrocatalysts are investigated as ORR catalysts. After synthesis via sacrificial SiO2 template method, the electrocatalyst exhibits much higher specific activity (1.88 mA/cm2) than a commercial Pt/C catalyst. The mass activity (0.49 A/mg) is 7 times higher than the commercial Pt/C catalyst. The kinetics of the ORR is evaluated using Tafel and K-L plots. It also exhibits a higher durability than commercial Pt/C catalyst during accelerated durability test (ADT). Moreover, the electrocatalyst shows good resistance against accelerated durability test for start–stop, the specific activity and mass activity drops 34.6% and 40.8%, respectively, far better than the commercial catalyst.
Dioscorea spp.: Comprehensive Review of Antioxidant Properties and Their Relation to Phytochemicals and Health Benefits
Authors: Adomėnienė Aušra , Venskutonis Petras Rimantas
Published: April 2022

Dioscorea, consisting of over 600 species, is the most important genus in the Dioscoreaceae family; however, the practically used plants, which are commonly called yam, are restricted to a remarkably smaller number of species. Numerous studies have reported the high nutritional value of yam, particularly as an alternative source of starch and some important micronutrients. Several Dioscorea species are widely used for various medicinal purposes as well. In many studies, the bioactivities and health benefits of Dioscorea extracts and other preparations have been related to the presence of phytochemicals, which possess antioxidant properties; they are related mainly to radical-scavenging capacity in chemical assays and positive effects on the endogenous antioxidant system in cell-based and in vivo assays. Considering the increasing number of publications on this topic and the absence of comprehensive and focused review papers on antioxidant potential, this article summarizes the results of studies on the antioxidant properties of Dioscorea spp. and their relation to phytochemicals and health benefits. A comprehensive survey of the published articles has revealed that the majority of studies have been performed with plant tubers (rhizomes, roots), while reports on leaves are rather scarce. In general, leaf extracts demonstrated stronger antioxidant potential than tuber preparations. This may be related to the differences in phytochemical composition: saponins, phenanthrenes and, for some pigment-rich species (purple yams), anthocyanins are important constituents in tubers, while phenolic acids and flavonoids are characteristic phytochemicals in the leaves. The review may assist in explaining ethnopharmacological knowledge on the health benefits of Dioscorea plants and their preparations; moreover, it may foster further studies of poorly investigated species, as well as their wider application in developing new functional foods and nutraceuticals.
Carotenoid Production from Microalgae: The Portuguese Scenario
Authors: Kholany Mariam , Coutinho João A. P. , Ventura Sónia P. M.
Published: April 2022

Microalgae have an outstanding capacity to efficiently produce value-added compounds. They have been inspiring researchers worldwide to develop a blue biorefinery, supporting the development of the bioeconomy, tackling the environmental crisis, and mitigating the depletion of natural resources. In this review, the characteristics of the carotenoids produced by microalgae are presented and the downstream processes developed to recover and purify them are analyzed, considering their main applications. The ongoing activities and initiatives taking place in Portugal regarding not only research, but also industrialization under the blue biorefinery concept are also discussed. The situation reported here shows that new techniques must be developed to make microalgae production more competitive. Downstream pigment purification technologies must be developed as they may have a considerable impact on the economic viability of the process. Government incentives are needed to encourage a constructive interaction between academics and businesses in order to develop a biorefinery that focuses on high-grade chemicals.
Impact of Different [Tc(N)PNP]-Scaffolds on the Biological Properties of the Small cRGDfK Peptide: Synthesis, In Vitro and In Vivo Evaluations
Authors: Salvarese Nicola , Carpanese Debora , Meléndez-Alafort Laura , De Nardo Laura , Calderan Andrea , Biondi Barbara , Ruzza Paolo , Rosato Antonio , Bolzati Cristina
Published: April 2022

Background: The [99mTc][Tc(N)(PNP)] system, where PNP is a bisphosphinoamine, is an interesting platform for the development of tumor ‘receptor-specific’ agents. Here, we compared the reactivity and impact of three [Tc(N)(PNP)] frameworks on the stability, receptor targeting properties, biodistribution, and metabolism of the corresponding [99mTc][Tc(N)(PNP)]-tagged cRGDfK peptide to determine the best performing agent and to select the framework useful for the preparation of [99mTc][Tc(N)(PNP)]-housing molecular targeting agents. Methods: cRGDfK pentapeptide was conjugated to Cys and labeled with each [Tc(N)(PNP)] framework. Radioconjugates were assessed for their lipophilicity, stability, in vitro and in vivo targeting properties, and performance. Results: All compounds were equally synthetically accessible and easy to purify (RCY ≥ 95%). The main influences of the synthon on the targeting peptide were observed in in vitro cell binding and in vivo. Conclusions: The variation in the substituents on the phosphorus atoms of the PNP enables a fine tuning of the biological features of the radioconjugates. ws[99mTc][Tc(N)(PNP3OH)]– and [99mTc][Tc(N)(PNP3)]– are better performing synthons in terms of labeling efficiency and in vivo performance than the [99mTc][Tc(N)(PNP43)] framework and are therefore more suitable for further radiopharmaceutical purposes. Furthermore, the good labeling properties of the ws[99mTc][Tc(N)(PNP3OH)]– framework can be exploited to extend this technology to the labeling of temperature-sensitive biomolecules suitable for SPECT imaging.
Phytochemical Composition and Protective Effect of Vernonanthura polyanthes Leaf against In Vivo Doxorubicin-Mediated Toxicity
Authors: Rocha Jamira Dias , Gallon Marilia Elias , de Melo Bisneto Abel Vieira , Santana Amaral Vanessa Cristiane , de Almeida Luciane Madureira , Borges Leonardo Luiz , Chen-Chen Lee , Gobbo-Neto Leonardo , Bailão Elisa Flávia Luiz Cardoso
Published: April 2022

Vernonanthura polyanthes (Spreng.) A.J. Vega & Dematt. (syn.: Vernonia polyanthes Less) is popularly known as “assa-peixe” and its leaves are used in folk medicine mainly to treat respiratory diseases. In this study, we evaluated the cytogenotoxic and anticytogenotoxic potential of the V. polyanthes leaf aqueous extract (VpLAE) and its n-butanol fraction (n-BF) in the presence or absence of doxorubicin (DXR) (pre-, co-, and post-treatments) on a murine model for 24 h or 120 h. The micronucleus test (MN) and the comet assay were used to assess the cytogenotoxic and anticytogenotoxic potential of VpLAE and n-BF (250, 500, and 1000 mg/kg) administered via gavage to Swiss Webster mice. The chemical profiles of VpLAE and n-BF were assessed by liquid chromatography coupled to mass spectrometry, and their metabolites were putatively identified. Lastly, the possible biological activities related to the (anti) cytogenotoxicity of the compounds were predicted using the PASS online webserver. The in vivo results showed that different doses of VpLAE and n-BF did not present cytotoxic activity; however, the MN test revealed a slight mutagenic activity for the 24 h treatments. Moderate genotoxic effects were demonstrated for all treatments in the comet assay. Regarding anticytotoxicity and antimutagenicity, VpLAE and n-BF presented a high cytoprotective potential against DXR toxic effects. In the co-treatment, VpLAE reduced the DXR genotoxicity by ~27%, and n-BF did not demonstrate antigenotoxic potential. In contrast, an antigenotoxic effect was observed for both VpLAE and n-BF in the pre- and post-treatments, reducing DXR genotoxicity by ~41% and ~47%, respectively. Chemical analysis of VpLAE and n-BF showed the presence of eight phenolic compounds, including seven chlorogenic acids and a flavonoid. The PASS online tool predicted antimutagenic, anticancer, antineoplastic, chemoprotective, antioxidant, and radical scavenging activities for all constituents identified in VpLAE and n-BF. V. polyanthes leaves presented a protective effect against DXR cytogenotoxicity. In general, VpLAE and n-BF showed a greater antigenotoxic potential in the pre- and post-treatments. The metabolites putatively identified in VpLAE and n-BF exhibited antioxidant and chemoprotective potential according to computational prediction analysis. Altogether, our results highlight the potential application of V. polyanthes to protect against toxic manifestations induced by DXR.
Thermal Catalytic-Cracking Low-Density Polyethylene Waste by Metakaolin-Based Geopolymer NaA Microsphere
Authors: Tang Shanshan , He Yan , Deng Xingfa , Cui Xuemin
Published: April 2022

Metakaolin-based geopolymer microspheres (MGM) with hierarchical pore structures were prepared by suspension dispersion method in dimethicone at 80 °C. The hydrothermal modification of MGM was carried out at a lower temperature of 80 °C, and a NaA molecular sieve converted from metakaolin-based geopolymer (NMGM) with good crystal structure was prepared and applied in thermal catalytic cracking of low-density polyethylene (LDPE) reaction. The one-pot two-stage thermal catalytic cracking of LDPE was carried out in a 100 mL micro-autoclave under normal pressure. In this work, the optimal proportions and optimal reaction conditions of catalysts for NMGM thermal catalytic cracking of LDPE waste to fuel oil were investigated. The NMGM catalyst showed high selectivity to the liquid product of thermal catalytic cracking of waste LDPE. Under the reaction conditions of reaction time of 1 h and reaction temperature of 400 °C, the liquid-phase yield of thermal catalytic cracking of LDPE reached a high of 88.45%, of which the content of gasoline components was 10.14% and the content of diesel components was 80.97%.
Covalent Reversible Inhibitors of Cysteine Proteases Containing the Nitrile Warhead: Recent Advancement in the Field of Viral and Parasitic Diseases
Authors: Brogi Simone , Ibba Roberta , Rossi Sara , Butini Stefania , Calderone Vincenzo , Gemma Sandra , Campiani Giuseppe
Published: April 2022

In the field of drug discovery, the nitrile group is well represented among drugs and biologically active compounds. It can form both non-covalent and covalent interactions with diverse biological targets, and it is amenable as an electrophilic warhead for covalent inhibition. The main advantage of the nitrile group as a warhead is mainly due to its milder electrophilic character relative to other more reactive groups (e.g., -CHO), reducing the possibility of unwanted reactions that would hinder the development of safe drugs, coupled to the ease of installation through different synthetic approaches. The covalent inhibition is a well-assessed design approach for serine, threonine, and cysteine protease inhibitors. The mechanism of hydrolysis of these enzymes involves the formation of a covalent acyl intermediate, and this mechanism can be exploited by introducing electrophilic warheads in order to mimic this covalent intermediate. Due to the relevant role played by the cysteine protease in the survival and replication of infective agents, spanning from viruses to protozoan parasites, we will review the most relevant and recent examples of protease inhibitors presenting a nitrile group that have been introduced to form or to facilitate the formation of a covalent bond with the catalytic cysteine active site residue.
Discovery of Bispecific Lead Compounds from Azadirachta indica against ZIKA NS2B-NS3 Protease and NS5 RNA Dependent RNA Polymerase Using Molecular Simulations
Authors: Kumar Sanjay , El-Kafrawy Sherif A. , Bharadwaj Shiv , Maitra S. S. , Alandijany Thamir A. , Faizo Arwa A. , Khateb Aiah M. , Dwivedi Vivek Dhar , Azhar Esam I.
Published: April 2022

Zika virus (ZIKV) has been characterized as one of many potential pathogens and placed under future epidemic outbreaks by the WHO. However, a lack of potential therapeutics can result in an uncontrolled pandemic as with other human pandemic viruses. Therefore, prioritized effective therapeutics development has been recommended against ZIKV. In this context, the present study adopted a strategy to explore the lead compounds from Azadirachta indica against ZIKV via concurrent inhibition of the NS2B-NS3 protease (ZIKVpro) and NS5 RNA dependent RNA polymerase (ZIKVRdRp) proteins using molecular simulations. Initially, structure-based virtual screening of 44 bioflavonoids reported in Azadirachta indica against the crystal structures of targeted ZIKV proteins resulted in the identification of the top four common bioflavonoids, viz. Rutin, Nicotiflorin, Isoquercitrin, and Hyperoside. These compounds showed substantial docking energy (−7.9 to −11.01 kcal/mol) and intermolecular interactions with essential residues of ZIKVpro (B:His51, B:Asp75, and B:Ser135) and ZIKVRdRp (Asp540, Ile799, and Asp665) by comparison to the reference compounds, O7N inhibitor (ZIKVpro) and Sofosbuvir inhibitor (ZIKVRdRp). Besides, long interval molecular dynamics simulation (500 ns) on the selected docked poses reveals stability of the respective docked poses contributed by intermolecular hydrogen bonds and hydrophobic interactions. The predicted complex stability was further supported by calculated end-point binding free energy using molecular mechanics generalized born surface area (MM/GBSA) method. Consequently, the identified common bioflavonoids are recommended as promising therapeutic inhibitors of ZIKVpro and ZIKVRdRp against ZIKV for further experimental assessment.
Effect of Tomato Peel Extract Grown under Drought Stress Condition in a Sarcopenia Model
Authors: Felice Francesca , Cesare Maria Michela , Fredianelli Luca , De Leo Marinella , Conti Veronica , Braca Alessandra , Di Stefano Rossella
Published: April 2022

Tomatoes and their derivates represent an important source of natural biologically active components. The present study aims to investigate the protective effect of tomato peel extracts, grown in normal (RED-Ctr) or in drought stress (RED-Ds) conditions, on an experimental model of sarcopenia. The phenolic profile and total polyphenols content (TPC) of RED-Ctr and RED-Ds were determined by Ultra High-Performance Liquid Chromatography (UHPLC) analyses coupled to electrospray ionization high-resolution mass spectrometry (ESI-HR-MS). Human skeletal muscle myoblasts (HSMM) were differentiated in myotubes, and sarcopenia was induced by dexamethasone (DEXA) treatment. Differentiation and sarcopenia were evaluated by both real-time PCR and immunofluorescent techniques. Data show that myosin heavy chain 2 (MYH2), troponin T (TNNT1), and miogenin (MYOG) were expressed in differentiated myotubes. 5 μg Gallic Acid Equivalent (GAE/mL) of TPC from RED-Ds extract significantly reduced muscle atrophy induced by DEXA. Moreover, Forkhead BoxO1 (FOXO1) expression, involved in cell atrophy, was significantly decreased by RED-Ds extract. The protective effect of tomato peel extracts depended on their qualitative polyphenolic composition, resulting effectively in the in vitro model of sarcopenia.