bluuebunny/crossref_metadata_2025 huggingface dataset:
Supplementary Table 1 from Impaired Long-Term Expansion and Self-Renewal Potential of Pediatric Acute Myeloid Leukemia–Initiating Cells By PTK787/ZK 222584
Authors: Weidenaar Alida C. , ter Elst Arja , Kampen Kim R. , Meeuwsen-de Boer Tiny , Kamps Willem A. , Schuringa Jan Jacob , de Bont Eveline S.J.M.
Published: April 2023

XLSX file - 21K, Specific primers for RT-PCR.
Supplementary Figures 1 - 8, Tables 1 - 2 from mda-7/IL-24 Expression Inhibits Breast Cancer through Upregulation of Growth Arrest-Specific Gene 3 (gas3) and Disruption of β1 Integrin Function
Authors: Li You-Jun , Liu Guodong , Li Yanmei , Vecchiarelli-Federico Laura M. , Liu Jeff C. , Zacksenhaus Eldad , Shan Sze W. , Yang Burton B. , Li Qi , Dash Rupesh , Fisher Paul B. , Archer Michael C. , Ben-David Yaacov
Published: April 2023

PDF file - 5906K, Supplemental Table I - Inhibition of mammary carcinogenesis in Wistar-Furth rats by mda-7/IL-24 Supplemental Table II - Microarray analysis of differential gene expression in FE1.2 + IL-24S compared to FE1.2 + IL-24AS cells. Supplemental Figure 1: Serum starvation induces GAS3 expression in both FE1.2 and FE1.3 cells. Supplemental Figure 2: GAS3 downregulation decreases the growth of cells in soft agar. Supplemental Figure 3: STAT3 inhibition reduced attachment of attaches cells to culture plates. Supplemental Figure 4: GAS3 expression reduced attachment of confluent cells to culture plates. Supplemental Figure 5: Binding of GAS3 to β1 integrin using immunoprecipitation assay. Supplemental Figure 6: GAS3 expression inhibits attachment to and proliferation on fibronectin coated culture plates. Supplemental Figure 7: GAS3 expression increases attachment to laminin coated culture plates. Supplemental Figure 8: GAS3 expression by immunohistochemistry.
Supplementary Tables 1-4 from SOX9 Is Essential for Triple-Negative Breast Cancer Cell Survival and Metastasis
Authors: Ma Yanxia , Shepherd Jonathan , Zhao Dekuang , Bollu Lakshmi Reddy , Tahaney William M. , Hill Jamal , Zhang Yun , Mazumdar Abhijit , Brown Powel H.
Published: April 2023

Supplementary Table 1. Primers and probes for qRT-PCR assay. Supplementary Table 2. Primers for CHIP assay (positive binding sequence). Supplementary Table 3. Primers for CHIP assay (negative binding sequence). Supplementary Table 4. Primers for H3K27Ac/Me3 CHIP assay (binding peak and negative binding sequence).
Supplementary Figure 7 from SOX9 Is Essential for Triple-Negative Breast Cancer Cell Survival and Metastasis
Authors: Ma Yanxia , Shepherd Jonathan , Zhao Dekuang , Bollu Lakshmi Reddy , Tahaney William M. , Hill Jamal , Zhang Yun , Mazumdar Abhijit , Brown Powel H.
Published: April 2023

SOX9 expression level regulates cell migration and invasion in breast TNBC and Non-TNBC cells
Supplementary Figure 3 from SOX9 Is Essential for Triple-Negative Breast Cancer Cell Survival and Metastasis
Authors: Ma Yanxia , Shepherd Jonathan , Zhao Dekuang , Bollu Lakshmi Reddy , Tahaney William M. , Hill Jamal , Zhang Yun , Mazumdar Abhijit , Brown Powel H.
Published: April 2023

Effect of SOX family knockdown on breast cancer cell growth, migration and invasion
Figure S1-S9, Table S1 from Elevated Asparagine Biosynthesis Drives Brain Tumor Stem Cell Metabolic Plasticity and Resistance to Oxidative Stress
Authors: Thomas Tom M. , Miyaguchi Ken , Edwards Lincoln A. , Wang Hongqiang , Wollebo Hassen , Aiguo Li , Murali Ramachandran , Wang Yizhou , Braas Daniel , Michael Justin S. , Andres Allen M. , Zhang Miqin , Khalili Kamel , Gottlieb Roberta A. , Perez J. Manuel , Yu John S.
Published: April 2023

S1. Clinical and molecular profile of gliomas by ASNS status S2. CDKN2A and ASNS KM survival plots by IDH status S3. ASNS copy-number gain compared to ASNS mRNA & protein expression S4. IDH mutation inversely correlate to ASNS copy number gain S5. GSCs and ASNS expression levels Supp Table 1. Clinical & Molecular Profile for Parental GSC604 & GSC827 cell lines
Supplementary Figure 13 from SOX9 Is Essential for Triple-Negative Breast Cancer Cell Survival and Metastasis
Authors: Ma Yanxia , Shepherd Jonathan , Zhao Dekuang , Bollu Lakshmi Reddy , Tahaney William M. , Hill Jamal , Zhang Yun , Mazumdar Abhijit , Brown Powel H.
Published: April 2023

SOX9 binding peaks in the promoter area of apoptosis factors (1)
Supplementary Figure 11 from SOX9 Is Essential for Triple-Negative Breast Cancer Cell Survival and Metastasis
Authors: Ma Yanxia , Shepherd Jonathan , Zhao Dekuang , Bollu Lakshmi Reddy , Tahaney William M. , Hill Jamal , Zhang Yun , Mazumdar Abhijit , Brown Powel H.
Published: April 2023

Mechanism of SOX9 regulating breast cancer survival
Supplementary Figure 10 from SOX9 Is Essential for Triple-Negative Breast Cancer Cell Survival and Metastasis
Authors: Ma Yanxia , Shepherd Jonathan , Zhao Dekuang , Bollu Lakshmi Reddy , Tahaney William M. , Hill Jamal , Zhang Yun , Mazumdar Abhijit , Brown Powel H.
Published: April 2023

SOX9-CHIP Enrichment Analysis by NIH PAVIS Tool
Supplementary Figure 1 - Part 1 from SOX9 Is Essential for Triple-Negative Breast Cancer Cell Survival and Metastasis
Authors: Ma Yanxia , Shepherd Jonathan , Zhao Dekuang , Bollu Lakshmi Reddy , Tahaney William M. , Hill Jamal , Zhang Yun , Mazumdar Abhijit , Brown Powel H.
Published: April 2023

The association of SOX transcription factors expression level and breast cancer subtypes
Supplementary Data from SOX9 Is Essential for Triple-Negative Breast Cancer Cell Survival and Metastasis
Authors: Ma Yanxia , Shepherd Jonathan , Zhao Dekuang , Bollu Lakshmi Reddy , Tahaney William M. , Hill Jamal , Zhang Yun , Mazumdar Abhijit , Brown Powel H.
Published: April 2023

Supplementary Figure Legends 1-16 and Supplementary Table Legends 1-4
Supplementary Table 2-3 from MnSOD Promotes Tumor Invasion via Upregulation of FoxM1–MMP2 Axis and Related with Poor Survival and Relapse in Lung Adenocarcinomas
Authors: Chen Po-Ming , Wu Tzu-Chin , Shieh Shwn-Huey , Wu Yi-Hui , Li Min-Chin , Sheu Gwo-Tarng , Cheng Ya-Wen , Chen Chih-Yi , Lee Huei
Published: April 2023

Supplementary Table 2 PDF file - 144K, List of primer sequences (5'�|��3') and their reaction conditions used in the present study
Supplementary Methods from The Novel miR-7515 Decreases the Proliferation and Migration of Human Lung Cancer Cells by Targeting c-Met
Authors: Lee Ji Min , Yoo Jung Ki , Yoo Hanna , Jung Ho Yong , Lee Dong Ryul , Jeong Hye Cheol , Oh Seoung Hun , Chung Hyung Min , Kim Jin Kyeoung
Published: April 2023

PDF file - 121K
Supplementary Figure 4 from The Novel miR-7515 Decreases the Proliferation and Migration of Human Lung Cancer Cells by Targeting c-Met
Authors: Lee Ji Min , Yoo Jung Ki , Yoo Hanna , Jung Ho Yong , Lee Dong Ryul , Jeong Hye Cheol , Oh Seoung Hun , Chung Hyung Min , Kim Jin Kyeoung
Published: April 2023

PDF file - 49K, The expression of hc-smR-S2-5 in hc-smR-S2-5-transfected cells: The expression of hc-smR-S2-5 in A549 cells transfected with hc-smR-S2-5 or a negative control was determined by TaqMan miRNA Assay. (bars, SD; *, P < 0.05)
Figure S1 from Dual Inhibition of CDK4 and CDK2 via Targeting p27 Tyrosine Phosphorylation Induces a Potent and Durable Response in Breast Cancer Cells
Authors: Patel Priyank , Tsiperson Vladislav , Gottesman Susan R.S. , Somma Jonathan , Blain Stacy W.
Published: April 2023

Fig. S1 shows the data used to calculate IC50 concentrations for PD treatment of 4 cell lines, and the quantitation of data from Fig. 1C
Supplementary Figure S5 from miR-629 Targets TRIM33 to Promote TGFβ/Smad Signaling and Metastatic Phenotypes in ccRCC
Authors: Jingushi Kentaro , Ueda Yuko , Kitae Kaori , Hase Hiroaki , Egawa Hiroshi , Ohshio Ikumi , Kawakami Ryoji , Kashiwagi Yuri , Tsukada Yohei , Kobayashi Takumi , Nakata Wataru , Fujita Kazutoshi , Uemura Motohide , Nonomura Norio , Tsujikawa Kazutake
Published: April 2023

Supplementary Figure S5. A, Caki-2 cells were transfected with the miR-629 inhibitor or control miRNA inhibitor for 72 h, and then protein samples were collected and subjected to western blot analysis with anti-HNF4α antibody. The membrane was re-probed with the anti-β-tubulin antibody. The relative HNF4α expression is shown as mean {plus minus} S.D. of three independent experiments. B, Protein samples from ccRCC cell lines were collected and subjected to western blot analysis for HNF4α. The relative HNF4α expression is shown as means {plus minus} S.D. of three independent experiments. (C, D) The expression of HNF4α in ccRCC specimens was examined by immunohistochemical staining. One normal kidney tissue and 17 ccRCC samples were examined.
Supplementary Figure S from miR-629 Targets TRIM33 to Promote TGFβ/Smad Signaling and Metastatic Phenotypes in ccRCC
Authors: Jingushi Kentaro , Ueda Yuko , Kitae Kaori , Hase Hiroaki , Egawa Hiroshi , Ohshio Ikumi , Kawakami Ryoji , Kashiwagi Yuri , Tsukada Yohei , Kobayashi Takumi , Nakata Wataru , Fujita Kazutoshi , Uemura Motohide , Nonomura Norio , Tsujikawa Kazutake
Published: April 2023

Supplementary Figure S2. The Caki-2 cells were transfected with TRIM33 siRNA for 72 h, and then protein samples were collected and subjected to western blot analysis by using anti-TRIM33 antibody. The membrane was re-probed with the anti-β-tubulin antibody. Relative TRIM33 expression is shown as means {plus minus} S.D. of four independent experiments. **p < 0.01 vs. control siRNA.
Supplementary Figure 3 from The E3 Ligase CHIP Mediates p21 Degradation to Maintain Radioresistance
Authors: Biswas Kuntal , Sarkar Sukumar , Du Kangping , Brautigan David L. , Abbas Tarek , Larner James M.
Published: April 2023

DDR signaling after IR.
Supplementary Figure 1 from Heparin-like Polysaccharides Reduce Osteolytic Bone Destruction and Tumor Growth in a Mouse Model of Breast Cancer Bone Metastasis
Authors: Pollari Sirkku , Käkönen Rami S. , Mohammad Khalid S. , Rissanen Jukka P. , Halleen Jussi M. , Wärri Anni , Nissinen Liisa , Pihlavisto Marjo , Marjamäki Anne , Perälä Merja , Guise Theresa A. , Kallioniemi Olli , Käkönen Sanna-Maria
Published: April 2023

PDF file - 25K, Supplementary Figure 1. Basal and TGF-b-induced IL-11 production in parental MDA-MB-231 and highly metastatic MDA-MB-231(SA) cells. ***, P < 0.001 (t test).
Supplementary Data from Thrombin Regulates the Metastatic Potential of Human Rhabdomyosarcoma Cells: Distinct Role of PAR1 and PAR3 Signaling
Authors: Wysoczynski Marcin , Liu Rui , Kucia Magda , Drukala Justyna , Ratajczak Mariusz Z.
Published: April 2023

Supplementary Data from Thrombin Regulates the Metastatic Potential of Human Rhabdomyosarcoma Cells: Distinct Role of PAR1 and PAR3 Signaling
Supplementary Figure 2 from Breast Field Cancerization: Isolation and Comparison of Telomerase-Expressing Cells in Tumor and Tumor Adjacent, Histologically Normal Breast Tissue
Authors: Trujillo Kristina A. , Hines William C. , Vargas Keith M. , Jones Anna C. , Joste Nancy E. , Bisoffi Marco , Griffith Jeffrey K.
Published: April 2023

PDF file - 57K, hTERT promoter reporter (Green Vector) effectively separates mixed populations of cells based on telomerase activity. HEK293 cells (telomerase positive) and WI-38 cells (telomerase negative) were infected separately with "Green Virus", mixed in a 1:1 ratio and sorted based on "high" (R1) or "low" (R2) green fluorescence. Gel-based TRAP analysis was performed on sorted populations. Densitometry analysis demonstrated a 4.2 fold increase in telomerase activity in the High population.
Supplementary Figure 1 from NFBD1/MDC1 Regulates Cav1 and Cav2 Independently of DNA Damage and p53
Authors: Wilson Kathleen A. , Colavito Sierra A. , Schulz Vincent , Wakefield Patricia Heffernan , Sessa William , Tuck David , Stern David F.
Published: April 2023

Supplementary Figure 1 from NFBD1/MDC1 Regulates Cav1 and Cav2 Independently of DNA Damage and p53
Supplementary Data from Activated Platelets Provide a Functional Microenvironment for the Antiangiogenic Fragment of Histidine-Rich Glycoprotein
Authors: Thulin Åsa , Ringvall Maria , Dimberg Anna , Kårehed Karin , Väisänen Timo , Väisänen Marja-Riitta , Hamad Osama , Wang Jian , Bjerkvig Rolf , Nilsson Bo , Pihlajaniemi Taina , Åkerud Helena , Pietras Kristian , Jahnen-Dechent Wilhelm , Siegbahn Agneta , Olsson Anna-Karin
Published: April 2023

Supplementary Data from Activated Platelets Provide a Functional Microenvironment for the Antiangiogenic Fragment of Histidine-Rich Glycoprotein
Supplementary Data from Urinary-Type Plasminogen Activator Receptor/α3β1 Integrin Signaling, Altered Gene Expression, and Oral Tumor Progression
Authors: Ghosh Supurna , Koblinski Jennifer , Johnson Jeffrey , Liu Yueying , Ericsson Aaron , Davis J. Wade , Shi Zonggao , Ravosa Matthew J. , Crawford Susan , Frazier Shellaine , Stack M. Sharon
Published: April 2023

Supplementary Data from Urinary-Type Plasminogen Activator Receptor/α3β1 Integrin Signaling, Altered Gene Expression, and Oral Tumor Progression
Supplementary Data from Cyclooxygenase-2 Is a Novel Transcriptional Target of the Nuclear EGFR-STAT3 and EGFRvIII-STAT3 Signaling Axes
Authors: Lo Hui-Wen , Cao Xinyu , Zhu Hu , Ali-Osman Francis
Published: April 2023

Supplementary Data from Cyclooxygenase-2 Is a Novel Transcriptional Target of the Nuclear EGFR-STAT3 and EGFRvIII-STAT3 Signaling Axes
Supplementary figure from Activator Protein-1 Has an Essential Role in Pancreatic Cancer Cells and Is Regulated by a Novel Akt-Mediated Mechanism
Authors: Shin Sonyo , Asano Takayuki , Yao Yixin , Zhang Ronghua , Claret Francois-Xavier , Korc Murray , Sabapathy Kanaga , Menter David G. , Abbruzzese James L. , Reddy Shrikanth A.G.
Published: April 2023

Supplemental Figure 1
Supplementary Methods, Figure Legends 1-2 from Cancer Cell Dependence on Unsaturated Fatty Acids Implicates Stearoyl-CoA Desaturase as a Target for Cancer Therapy
Authors: Roongta Urvashi V. , Pabalan Jonathan G. , Wang Xinyu , Ryseck Rolf-Peter , Fargnoli Joseph , Henley Benjamin J. , Yang Wen-Pin , Zhu Jun , Madireddi Malavi T. , Lawrence R. Michael , Wong Tai W. , Rupnow Brent A.
Published: April 2023

PDF file - 70K
Supplementary Figure 2 from Cancer Cell Dependence on Unsaturated Fatty Acids Implicates Stearoyl-CoA Desaturase as a Target for Cancer Therapy
Authors: Roongta Urvashi V. , Pabalan Jonathan G. , Wang Xinyu , Ryseck Rolf-Peter , Fargnoli Joseph , Henley Benjamin J. , Yang Wen-Pin , Zhu Jun , Madireddi Malavi T. , Lawrence R. Michael , Wong Tai W. , Rupnow Brent A.
Published: April 2023

PDF file - 39K
Supplementary Figure 3 from Epithelial Protein-Tyrosine Phosphatase 1B Contributes to the Induction of Mammary Tumors by HER2/Neu but Is Not Essential for Tumor Maintenance
Authors: Balavenkatraman Kamal K. , Aceto Nicola , Britschgi Adrian , Mueller Urs , Bence Kendra K. , Neel Benjamin G. , Bentires-Alj Mohamed
Published: April 2023

PDF File (117K)
Supplementary Data from p34SEI-1 Inhibits Doxorubicin-Induced Senescence through a Pathway Mediated by Protein Kinase C-δ and c-Jun-NH2-Kinase 1 Activation in Human Breast Cancer MCF7 Cells
Authors: Lee Sae Lo Oom , Hong Seung-Woo , Shin Jae-Sik , Kim Jin Sun , Ko Seong-Gyu , Hong Nam-Joo , Kim Dae Jin , Lee Wang-Jae , Jin Dong-Hoon , Lee Myeong-Sok
Published: April 2023

Supplementary Data from p34SEI-1 Inhibits Doxorubicin-Induced Senescence through a Pathway Mediated by Protein Kinase C-δ and c-Jun-NH2-Kinase 1 Activation in Human Breast Cancer MCF7 Cells
Supplementary Data from Polycomb Group Protein Enhancer of Zeste 2 Is an Oncogene That Promotes the Neoplastic Transformation of a Benign Prostatic Epithelial Cell Line
Authors: Karanikolas Breanne D.W. , Figueiredo Marxa L. , Wu Lily
Published: April 2023

Supplementary Data from Polycomb Group Protein Enhancer of Zeste 2 Is an Oncogene That Promotes the Neoplastic Transformation of a Benign Prostatic Epithelial Cell Line
Supplementary Data from BAX/BAK–Independent Mitoptosis during Cell Death Induced by Proteasome Inhibition?
Authors: Lomonosova Elena , Ryerse Jan , Chinnadurai G.
Published: April 2023

Supplementary Data from BAX/BAK–Independent Mitoptosis during Cell Death Induced by Proteasome Inhibition?
Supplementary Data from The β1-Integrin–Dependent Function of RECK in Physiologic and Tumor Angiogenesis
Authors: Miki Takao , Shamma Awad , Kitajima Shunsuke , Takegami Yujiro , Noda Makoto , Nakashima Yasuaki , Watanabe Ken-ichiro , Takahashi Chiaki
Published: April 2023

Supplementary Data from The β1-Integrin–Dependent Function of RECK in Physiologic and Tumor Angiogenesis
Supplementary Figure 2 from Downregulation of Filamin A Interacting Protein 1-Like is Associated with Promoter Methylation and Induces an Invasive Phenotype in Ovarian Cancer
Authors: Burton Elizabeth R. , Gaffar Aneesa , Lee Soo Jin , Adeshuko Folashade , Whitney Kathleen D. , Chung Joon-Yong , Hewitt Stephen M. , Huang Gloria S. , Goldberg Gary L. , Libutti Steven K. , Kwon Mijung
Published: April 2023

PDF file - 159K
Supplementary Figure 1 from Downregulation of Filamin A Interacting Protein 1-Like is Associated with Promoter Methylation and Induces an Invasive Phenotype in Ovarian Cancer
Authors: Burton Elizabeth R. , Gaffar Aneesa , Lee Soo Jin , Adeshuko Folashade , Whitney Kathleen D. , Chung Joon-Yong , Hewitt Stephen M. , Huang Gloria S. , Goldberg Gary L. , Libutti Steven K. , Kwon Mijung
Published: April 2023

PDF file - 123K
Supplementary Data from Differential Influence of Normal and Cancer-Associated Fibroblasts on the Growth of Human Epithelial Cells in an In vitro Cocultivation Model of Prostate Cancer
Authors: Paland Nicole , Kamer Iris , Kogan-Sakin Ira , Madar Shalom , Goldfinger Naomi , Rotter Varda
Published: April 2023

Supplementary Data from Differential Influence of Normal and Cancer-Associated Fibroblasts on the Growth of Human Epithelial Cells in an In vitro Cocultivation Model of Prostate Cancer
Supplementary Data from Role of Fibulin-5 in Metastatic Organ Colonization
Authors: Devitt Møller Henrik , Ralfkjær Ulrik , Cremers Natascha , Frankel Mika , Troelsgaard Pedersen Rune , Klingelhöfer Jörg , Yanagisawa Hiromi , Grigorian Mariam , Guldberg Per , Sleeman Jonathan , Lukanidin Eugene , Ambartsumian Noona
Published: April 2023

Supplementary Figures S1-S4; Supplementary Materials and Methods; Supplementary Table S1.
Supplementary Data from Identification of a Novel DNA Binding Site and a Transcriptional Target for Activating Transcription Factor 5 in C6 Glioma and MCF-7 Breast Cancer Cells
Authors: Li Guangfu , Li Wenhong , Angelastro James M. , Greene Lloyd A. , Liu David X.
Published: April 2023

Supplementary Data from Identification of a Novel DNA Binding Site and a Transcriptional Target for Activating Transcription Factor 5 in C6 Glioma and MCF-7 Breast Cancer Cells
Supplementary Figures 1-4 from Notch Signaling Contributes to Lung Cancer Clonogenic Capacity In Vitro but May Be Circumvented in Tumorigenesis In Vivo
Authors: Osanyingbemi-Obidi Joyce , Dobromilskaya Irina , Illei Peter B. , Hann Christine L. , Rudin Charles M.
Published: April 2023

PDF file - 534K
Supplementary Data from Overexpression of miR-21 Promotes an In vitro Metastatic Phenotype by Targeting the Tumor Suppressor RHOB
Authors: Connolly Erin C. , Van Doorslaer Koenraad , Rogler Leslie E. , Rogler Charles E.
Published: April 2023

Supplementary Data from Overexpression of miR-21 Promotes an In vitro Metastatic Phenotype by Targeting the Tumor Suppressor RHOB
Supplementary Data from Up-Regulation of Krüppel-Like Factor 5 in Pancreatic Cancer Is Promoted by Interleukin-1β Signaling and Hypoxia-Inducible Factor-1α
Authors: Mori Akira , Moser Christian , Lang Sven A. , Hackl Christina , Gottfried Eva , Kreutz Marina , Schlitt Hans J. , Geissler Edward K. , Stoeltzing Oliver
Published: April 2023

Supplementary Data from Up-Regulation of Krüppel-Like Factor 5 in Pancreatic Cancer Is Promoted by Interleukin-1β Signaling and Hypoxia-Inducible Factor-1α
Supplementary Figure 4 from EGFR Signals Downregulate Tumor Suppressors miR-143 and miR-145 in Western Diet–Promoted Murine Colon Cancer: Role of G1 Regulators
Authors: Zhu Hongyan , Dougherty Urszula , Robinson Victoria , Mustafi Reba , Pekow Joel , Kupfer Sonia , Li Yan-Chun , Hart John , Goss Kathleen , Fichera Alessandro , Joseph Loren , Bissonnette Marc
Published: April 2023

Supplementary Figure 4 from EGFR Signals Downregulate Tumor Suppressors miR-143 and miR-145 in Western Diet–Promoted Murine Colon Cancer: Role of G1 Regulators
Supplementary Data from Matrix Metalloproteinase–Activated Anthrax Lethal Toxin Inhibits Endothelial Invasion and Neovasculature Formation during In vitro Morphogenesis
Authors: Alfano Randall W. , Leppla Stephen H. , Liu Shihui , Bugge Thomas H. , Meininger Cynthia J. , Lairmore Terry C. , Mulne Arlynn F. , Davis Samuel H. , Duesbery Nicholas S. , Frankel Arthur E.
Published: April 2023

Supplementary Data from Matrix Metalloproteinase–Activated Anthrax Lethal Toxin Inhibits Endothelial Invasion and Neovasculature Formation during In vitro Morphogenesis
Supplementary Table S1 from Targeting Activating Transcription Factor 3 by Galectin-9 Induces Apoptosis and Overcomes Various Types of Treatment Resistance in Chronic Myelogenous Leukemia
Authors: Kuroda Junya , Yamamoto Mio , Nagoshi Hisao , Kobayashi Tsutomu , Sasaki Nana , Shimura Yuji , Horiike Shigeo , Kimura Shinya , Yamauchi Akira , Hirashima Mitsuomi , Taniwaki Masafumi
Published: April 2023

Supplementary Table S1.
Supplementary Figures 1-4 from MT1-MMP Cooperates with KrasG12D to Promote Pancreatic Fibrosis through Increased TGF-β Signaling
Authors: Krantz Seth B. , Shields Mario A. , Dangi-Garimella Surabhi , Cheon Eric C. , Barron Morgan R. , Hwang Rosa F. , Rao M. Sambasiva , Grippo Paul J. , Bentrem David J. , Munshi Hidayatullah G.
Published: April 2023

PDF File (6111K)
Supplementary Figure 2 from Vinculin Activators Target Integrins from Within the Cell to Increase Melanoma Sensitivity to Chemotherapy
Authors: Nelson Elke S. , Folkmann Andrew W. , Henry Michael D. , DeMali Kris A.
Published: April 2023

Supplementary Figure 2 from Vinculin Activators Target Integrins from Within the Cell to Increase Melanoma Sensitivity to Chemotherapy
Supplementary Figure 1 from Vinculin Activators Target Integrins from Within the Cell to Increase Melanoma Sensitivity to Chemotherapy
Authors: Nelson Elke S. , Folkmann Andrew W. , Henry Michael D. , DeMali Kris A.
Published: April 2023

Supplementary Figure 1 from Vinculin Activators Target Integrins from Within the Cell to Increase Melanoma Sensitivity to Chemotherapy
Supplementary Figure 1 from IKKβ Overexpression Leads to Pathologic Lesions in Stratified Epithelia and Exocrine Glands and to Tumoral Transformation of Oral Epithelia
Authors: Page Angustias , Cascallana José L. , Casanova M. Llanos , Navarro Manuel , Alameda Josefa P. , Pérez Paloma , Bravo Ana , Ramírez Angel
Published: April 2023

PDF file - 2.42MB, Phospho-p65 analysis in oral epithelia and exocrine glands of K5-IKKb transgenic mice. (A) Western blot analysis showing increased level of phospho-p65 (P-p65) in oral epithelia and exocrine glands of Tg mice (Forestom.: forestomach; prep. gl.: preputial glands; mamm. gl.: lactating mammary gland). Actin was used as loading control. (B) Immunohistochemical analysis of P-p65 in oral epithelia. In transgenic samples, the basal cell layer stained positive when using antibodies specific for IKKb and phospho-p65, both in forestomach and in palate epithelia (black arrowheads). In the staining conditions used, basal cells in WT samples were negative both for IKKb and P-p65 (white arrows). The antibody used for P-p65 detection in A and B is from Cell Signaling (3033). Bar: 100 mm.
Supplementary Data from Reciprocal Effects of STAT5 and STAT3 in Breast Cancer
Authors: Walker Sarah R. , Nelson Erik A. , Zou Lihua , Chaudhury Mousumi , Signoretti Sabina , Richardson Andrea , Frank David A.
Published: April 2023

Supplementary Data from Reciprocal Effects of STAT5 and STAT3 in Breast Cancer
Supplementary Data from Altered-Function p53 Missense Mutations Identified in Breast Cancers Can Have Subtle Effects on Transactivation
Authors: Jordan Jennifer J. , Inga Alberto , Conway Kathleen , Edmiston Sharon , Carey Lisa A. , Wu Lin , Resnick Michael A.
Published: April 2023

Supplementary Data from Altered-Function p53 Missense Mutations Identified in Breast Cancers Can Have Subtle Effects on Transactivation
Supplementary Methods, Figures 1-4 from Forced Dimerization Increases the Activity of ΔEGFR/EGFRvIII and Enhances Its Oncogenicity
Authors: Hwang Yeohyeon , Chumbalkar Vaibhav , Latha Khatri , Bogler Oliver
Published: April 2023

PDF file - 128K
Supplementary Figure 4 from Forced Dimerization Increases the Activity of ΔEGFR/EGFRvIII and Enhances Its Oncogenicity
Authors: Hwang Yeohyeon , Chumbalkar Vaibhav , Latha Khatri , Bogler Oliver
Published: April 2023

PDF file - 2255K
Supplementary Figures S1-S2 from Loss of p53 and MCT-1 Overexpression Synergistically Promote Chromosome Instability and Tumorigenicity
Authors: Kasiappan Ravi , Shih Hung-Ju , Chu Kang-Lin , Chen Wei-Ti , Liu Hui-Ping , Huang Shiu-Feng , Choy Chik On , Shu Chung-Li , Din Richard , Chu Jan-Show , Hsu Hsin-Ling
Published: April 2023

Supplementary Figures S1-S2 from Loss of p53 and MCT-1 Overexpression Synergistically Promote Chromosome Instability and Tumorigenicity
Supplementary Figure 1 from ADAM12 Produced by Tumor Cells Rather than Stromal Cells Accelerates Breast Tumor Progression
Authors: Fröhlich Camilla , Nehammer Camilla , Albrechtsen Reidar , Kronqvist Pauliina , Kveiborg Marie , Sehara-Fujisawa Atsuko , Mercurio Arthur M. , Wewer Ulla M.
Published: April 2023

PDF file - 213K, A Pan-cytokeratin expression (green) in immortalized PyMT-A12hz and PyMT-A12null tumor cells. Blue represents DAPI staining. Bar = 12 �m. B. Mean (�SD) total tumor burden on FVB/N mice injected subcutaneously in the right flank with PyM-A12hz (n=11) or PyMT-A12null (n=15) tumor cells. *P < 0.05
Supplementary Figure 4 from Adaptive Basal Phosphorylation of eIF2α Is Responsible for Resistance to Cellular Stress–Induced Cell Death in Pten-Null Hepatocytes
Authors: Zeng Ni , Li Yang , He Lina , Xu Xiaoling , Galicia Vivian , Deng Chuxia , Stiles Bangyan L.
Published: April 2023

PDF file - 2.8MB
Supplementary Data from Sustained c-Jun-NH2-Kinase Activity Promotes Epithelial-Mesenchymal Transition, Invasion, and Survival of Breast Cancer Cells by Regulating Extracellular Signal-Regulated Kinase Activation
Authors: Wang Jinhua , Kuiatse Isere , Lee Adrian V. , Pan Jingxuan , Giuliano Armando , Cui Xiaojiang
Published: April 2023

Supplementary Data from Sustained c-Jun-NH2-Kinase Activity Promotes Epithelial-Mesenchymal Transition, Invasion, and Survival of Breast Cancer Cells by Regulating Extracellular Signal-Regulated Kinase Activation
Supplementary Methods from Methyl-Binding Domain Protein 2–Dependent Proliferation and Survival of Breast Cancer Cells
Authors: Mian Omar Y. , Wang Shou Zhen , Zhu Sheng Zu , Gnanapragasam Merlin N. , Graham Laura , Bear Harry D. , Ginder Gordon D.
Published: April 2023

PDF file - 147K
Supplementary Figure 1 from Methyl-Binding Domain Protein 2–Dependent Proliferation and Survival of Breast Cancer Cells
Authors: Mian Omar Y. , Wang Shou Zhen , Zhu Sheng Zu , Gnanapragasam Merlin N. , Graham Laura , Bear Harry D. , Ginder Gordon D.
Published: April 2023

PDF file - 253K
Supplementary Data from Mutations in the Circadian Gene CLOCK in Colorectal Cancer
Authors: Alhopuro Pia , Björklund Mikael , Sammalkorpi Heli , Turunen Mikko , Tuupanen Sari , Biström Mia , Niittymäki Iina , Lehtonen Heli J. , Kivioja Teemu , Launonen Virpi , Saharinen Juha , Nousiainen Kari , Hautaniemi Sampsa , Nuorva Kyösti , Mecklin Jukka-Pekka , Järvinen Heikki , Orntoft Torben , Arango Diego , Lehtonen Rainer , Karhu Auli , Taipale Jussi , Aaltonen Lauri A.
Published: April 2023

Supplementary Figures S1-S3 and Supplementary Tables S1-S3.
Supplementary Data from Bcl-2 Modulation to Activate Apoptosis in Prostate Cancer
Authors: Bray Kevin , Chen Hsin-Yi , Karp Cristina M. , May Michael , Ganesan Shridar , Karantza-Wadsworth Vassiliki , DiPaola Robert S. , White Eileen
Published: April 2023

Supplementary Data from Bcl-2 Modulation to Activate Apoptosis in Prostate Cancer
Supplementary Figure S1 from Bortezomib-Resistant Nuclear Factor-κB Activity in Multiple Myeloma Cells
Authors: Markovina Stephanie , Callander Natalie S. , O'Connor Shelby L. , Kim Jihoon , Werndli Jae E. , Raschko Martha , Leith Catherine P. , Kahl Brad S. , Kim KyungMann , Miyamoto Shigeki
Published: April 2023

Supplementary Figure S1 from Bortezomib-Resistant Nuclear Factor-κB Activity in Multiple Myeloma Cells
Supplementary Data from Down-Regulation of 3-Phosphoinositide–Dependent Protein Kinase-1 Levels Inhibits Migration and Experimental Metastasis of Human Breast Cancer Cells
Authors: Liu Ying , Wang Jingna , Wu Min , Wan Wuzhou , Sun Ronghua , Yang De , Sun Xiangjun , Ma Dalong , Ying Guoguang , Zhang Ning
Published: April 2023

Supplementary Data from Down-Regulation of 3-Phosphoinositide–Dependent Protein Kinase-1 Levels Inhibits Migration and Experimental Metastasis of Human Breast Cancer Cells
Supplementary Methods, Figures 1-13, Tables 1-2 from Temporal and Spatial Cooperation of Snail1 and Twist1 during Epithelial–Mesenchymal Transition Predicts for Human Breast Cancer Recurrence
Authors: Tran David D. , Corsa Callie Ann S. , Biswas Hirak , Aft Rebecca L. , Longmore Gregory D.
Published: April 2023

PDF file - 9.8MB, Temporal and Spatial cooperation of Snail1 and Twist1 during Epithelial-Mesenchymal Transition predicts for human breast cancer recurrence.
Supplementary Data from Extracellular Matrix-Induced Gene Expression in Human Breast Cancer Cells
Authors: Garamszegi Nandor , Garamszegi Susanna P. , Shehadeh Lina A. , Scully Sean P.
Published: April 2023

Supplementary Data from Extracellular Matrix-Induced Gene Expression in Human Breast Cancer Cells
Supplementary Figures S1-S7 from Mechanisms of Drug Sensitization to TRA-8, an Agonistic Death Receptor 5 Antibody, Involve Modulation of the Intrinsic Apoptotic Pathway in Human Breast Cancer Cells
Authors: Amm Hope M. , Zhou Tong , Steg Adam D. , Kuo Huichien , Li Yufeng , Buchsbaum Donald J.
Published: April 2023

Supplementary Figures S1-S7.
Supplementary Figure 3 from Ablation of Sphingosine Kinase-2 Inhibits Tumor Cell Proliferation and Migration
Authors: Gao Peng , Smith Charles D.
Published: April 2023

PDF file - 38K
Supplementary Figure 2 from Ablation of Sphingosine Kinase-2 Inhibits Tumor Cell Proliferation and Migration
Authors: Gao Peng , Smith Charles D.
Published: April 2023

PDF file - 76K
Supplementary Figure 1 from Ablation of Sphingosine Kinase-2 Inhibits Tumor Cell Proliferation and Migration
Authors: Gao Peng , Smith Charles D.
Published: April 2023

PDF file - 114K
Supplementary Data from Suppression of the uPAR–uPA System Retards Angiogenesis, Invasion, and In Vivo Tumor Development in Pancreatic Cancer Cells
Authors: Gorantla Bharathi , Asuthkar Swapna , Rao Jasti S. , Patel Jitendra , Gondi Christopher S.
Published: April 2023

Supplementary Data from Suppression of the uPAR–uPA System Retards Angiogenesis, Invasion, and In Vivo Tumor Development in Pancreatic Cancer Cells
Supplementary Data from Matrix Protein CCN1 Is Critical for Prostate Carcinoma Cell Proliferation and TRAIL-Induced Apoptosis
Authors: Franzen Carrie A. , Chen Chih-Chiun , Todorović Viktor , Juric Vladislava , Monzon Ricardo I. , Lau Lester F.
Published: April 2023

Supplementary Data from Matrix Protein CCN1 Is Critical for Prostate Carcinoma Cell Proliferation and TRAIL-Induced Apoptosis
Supplementary Figures 1-6 from Downregulation of Sec23A Protein by miRNA-375 in Prostate Carcinoma
Authors: Szczyrba Jaroslaw , Nolte Elke , Wach Sven , Kremmer Elisabeth , Stöhr Robert , Hartmann Arndt , Wieland Wolf , Wullich Bernd , Grässer Friedrich A.
Published: April 2023

Supplementary Figures 1-6 from Downregulation of Sec23A Protein by miRNA-375 in Prostate Carcinoma
Supplementary Data from Next Generation Sequencing of Serum Circulating Nucleic Acids from Patients with Invasive Ductal Breast Cancer Reveals Differences to Healthy and Nonmalignant Controls
Authors: Beck Julia , Urnovitz Howard B. , Mitchell William M. , Schütz Ekkehard
Published: April 2023

Supplementary Data from Next Generation Sequencing of Serum Circulating Nucleic Acids from Patients with Invasive Ductal Breast Cancer Reveals Differences to Healthy and Nonmalignant Controls
Supplementary Figure 6 from Lrig1 Is an Estrogen-Regulated Growth Suppressor and Correlates with Longer Relapse-Free Survival in ERα-Positive Breast Cancer
Authors: Krig Sheryl R. , Frietze Seth , Simion Catalina , Miller Jamie K. , Fry Will H.D. , Rafidi Hanine , Kotelawala Lakmal , Qi Lihong , Griffith Obi L. , Gray Joe W. , Carraway Kermit L. , Sweeney Colleen
Published: April 2023

PDF file - 1.57MB
Supplementary Figure 4 from Resveratrol Inhibits Proliferation and Survival of Epstein Barr Virus–Infected Burkitt's Lymphoma Cells Depending on Viral Latency Program
Authors: De Leo Alessandra , Arena Giuseppe , Stecca Claudia , Raciti Marisa , Mattia Elena
Published: April 2023

PDF File - (840K) - Resveratrol-induced inhibition of LCLs proliferation. Cells were incubated with RV at the concentrations indicated. After 24, 48 and 72 hours, cell counts were assessed by trypan blue staining and the values expressed as percentage of control cells treated with the diluent. Each point represents the mean � SD of three similar experiments.
Supplementary Figure 3 from Resveratrol Inhibits Proliferation and Survival of Epstein Barr Virus–Infected Burkitt's Lymphoma Cells Depending on Viral Latency Program
Authors: De Leo Alessandra , Arena Giuseppe , Stecca Claudia , Raciti Marisa , Mattia Elena
Published: April 2023

PDF File - (1546K) - AP1 activity in resveratrol-treated BL cells. (a) At the indicated times (hours), whole-cell extracts were incubated with a consensus AP1 binding site oligonucleotide to evaluate AP1 activity by EMSA (see Methods).
Supplementary Table S1 from JAG2 Induction in Hypoxic Tumor Cells Alters Notch Signaling and Enhances Endothelial Cell Tube Formation
Authors: Pietras Alexander , von Stedingk Kristoffer , Lindgren David , Påhlman Sven , Axelson Håkan
Published: April 2023

Supplementary Table S1 from JAG2 Induction in Hypoxic Tumor Cells Alters Notch Signaling and Enhances Endothelial Cell Tube Formation
Supplementary Figure Legends from Down Syndrome Candidate Region 1 Isoform 1 Mediates Angiogenesis through the Calcineurin-NFAT Pathway
Authors: Qin Liuliang , Zhao Dezheng , Liu Xin , Nagy Janice A. , Hoang Mien Van , Brown Lawrence F. , Dvorak Harold F. , Zeng Huiyan
Published: April 2023

Supplementary Figure Legends from Down Syndrome Candidate Region 1 Isoform 1 Mediates Angiogenesis through the Calcineurin-NFAT Pathway
Supplementary Table S1 from Histone Deacetylase Inhibitors (HDI) Cause DNA Damage in Leukemia Cells: A Mechanism for Leukemia-Specific HDI-Dependent Apoptosis?
Authors: Gaymes Terry J. , Padua Rose Ann , Pla Marika , Orr Stephen , Omidvar Nader , Chomienne Christine , Mufti Ghulam J. , Rassool Feyruz V.
Published: April 2023

Supplementary Table S1 from Histone Deacetylase Inhibitors (HDI) Cause DNA Damage in Leukemia Cells: A Mechanism for Leukemia-Specific HDI-Dependent Apoptosis?
Supplementary Table S3 from Identification and Validation of Colorectal Neoplasia–Specific Methylation Markers for Accurate Classification of Disease
Authors: Model Fabian , Osborn Neal , Ahlquist David , Gruetzmann Robert , Molnar Bela , Sipos Ferenc , Galamb Orsolya , Pilarsky Christian , Saeger Hans-Detlev , Tulassay Zsolt , Hale Kari , Mooney Suzanne , Lograsso Joseph , Adorjan Peter , Lesche Ralf , Dessauer Andreas , Kleiber Joerg , Porstmann Baerbel , Sledziewski Andrew , Lofton-Day Catherine
Published: April 2023

Supplementary Table S3 from Identification and Validation of Colorectal Neoplasia–Specific Methylation Markers for Accurate Classification of Disease
Supplementary Figures S1-S2 from Gene Expression Changes in an Animal Melanoma Model Correlate with Aggressiveness of Human Melanoma Metastases
Authors: Xu Lei , Shen Steven S. , Hoshida Yujin , Subramanian Aravind , Ross Ken , Brunet Jean-Philippe , Wagner Stephan N. , Ramaswamy Sridhar , Mesirov Jill P. , Hynes Richard O.
Published: April 2023

Supplementary Figures S1-S2 from Gene Expression Changes in an Animal Melanoma Model Correlate with Aggressiveness of Human Melanoma Metastases
Supplementary Data from Interferon Regulatory Factors IRF5 and IRF7 Inhibit Growth and Induce Senescence in Immortal Li-Fraumeni Fibroblasts
Authors: Li Qunfang , Tang Lin , Roberts Paul Christopher , Kraniak Janice M. , Fridman Aviva Levine , Kulaeva Olga I. , Tehrani Omid S. , Tainsky Michael A.
Published: April 2023

Supplementary Data from Interferon Regulatory Factors IRF5 and IRF7 Inhibit Growth and Induce Senescence in Immortal Li-Fraumeni Fibroblasts
Supplementary Data from Galectin-3 Targeted Therapy with a Small Molecule Inhibitor Activates Apoptosis and Enhances Both Chemosensitivity and Radiosensitivity in Papillary Thyroid Cancer
Authors: Lin Chi-Iou , Whang Edward E. , Donner David B. , Jiang Xiaofeng , Price Brendan D. , Carothers Adelaide M. , Delaine Tamara , Leffler Hakon , Nilsson Ulf J. , Nose Vania , Moore, Francis D. , Ruan Daniel T.
Published: April 2023

Supplementary Data from Galectin-3 Targeted Therapy with a Small Molecule Inhibitor Activates Apoptosis and Enhances Both Chemosensitivity and Radiosensitivity in Papillary Thyroid Cancer
Supplementary Data 3 from NR0B1 Is Required for the Oncogenic Phenotype Mediated by EWS/FLI in Ewing's Sarcoma
Authors: Kinsey Michelle , Smith Richard , Lessnick Stephen L.
Published: April 2023

CLS file
Supplementary Table 1 from Molecular Characterization of the Transition to Malignancy in a Genetically Engineered Mouse-Based Model of Ductal Carcinoma In situ1
Authors: Namba Ruria , Maglione Jeannie E. , Young Lawrence J.T. , Borowsky Alexander D. , Cardiff Robert D. , MacLeod Carol L. , Gregg Jeffrey P.
Published: April 2023

Supplementary Table 1 from Molecular Characterization of the Transition to Malignancy in a Genetically Engineered Mouse-Based Model of Ductal Carcinoma In situ1
Supplementary Data from Induction of Tumorigenesis and Metastasis by the Murine Orthologue of Tumor Protein D52
Authors: Lewis Jennifer D. , Payton Laura A. , Whitford Jill G. , Byrne Jennifer A. , Smith David I. , Yang LiBang , Bright Robert K.
Published: April 2023

Supplementary Data from Induction of Tumorigenesis and Metastasis by the Murine Orthologue of Tumor Protein D52
Supplementary Figures 1-4 from Comprehensive Profiling of 8p11-12 Amplification in Breast Cancer
Authors: Gelsi-Boyer Véronique , Orsetti Béatrice , Cervera Nathalie , Finetti Pascal , Sircoulomb Fabrice , Rougé Carole , Lasorsa Laurence , Letessier Anne , Ginestier Christophe , Monville Florence , Esteyriès Séverine , Adélaïde José , Esterni Benjamin , Henry Catherine , Ethier Stephen P. , Bibeau Frédéric , Mozziconacci Marie-Joëlle , Charafe-Jauffret Emmanuelle , Jacquemier Jocelyne , Bertucci François , Birnbaum Daniel , Theillet Charles , Chaffanet Max
Published: April 2023

Supplementary Figures 1-4 from Comprehensive Profiling of 8p11-12 Amplification in Breast Cancer
Data from Multi-substrate Metabolic Tracing Reveals Marked Heterogeneity and Dependency on Fatty Acid Metabolism in Human Prostate Cancer
Authors: Fidelito Gio , De Souza David P. , Niranjan Birunthi , De Nardo William , Keerthikumar Shivakumar , Brown Kristin , Taylor Renea A. , Watt Matthew J.
Published: April 2023

Abstract
Cancer cells undergo metabolic reprogramming to meet increased bioenergetic demands. Studies in cells and mice have highlighted the importance of oxidative metabolism and lipogenesis in prostate cancer; however, the metabolic landscape of human prostate cancer remains unclear. To address this knowledge gap, we performed radiometric (14C) and stable (13C) isotope tracing assays in precision-cut slices of patient-derived xenografts (PDX). Glucose, glutamine, and fatty acid oxidation was variably upregulated in malignant PDXs compared with benign PDXs. De novo lipogenesis (DNL) and storage of free fatty acids into phospholipids and triacylglycerols were increased in malignant PDXs. There was no difference in substrate utilization between localized and metastatic PDXs and hierarchical clustering revealed marked metabolic heterogeneity across all PDXs. Mechanistically, glucose utilization was mediated by acetyl-CoA production rather than carboxylation of pyruvate, while glutamine entered the tricarboxylic acid cycle through transaminase reactions before being utilized via oxidative or reductive pathways. Blocking fatty acid uptake or fatty acid oxidation with pharmacologic inhibitors was sufficient to reduce cell viability in PDX-derived organoids, whereas blockade of DNL, or glucose or glutamine oxidation induced variable and limited therapeutic efficacy. These findings demonstrate that human prostate cancer, irrespective of disease stage, can effectively utilize all metabolic substrates, albeit with marked heterogeneity across tumors. We also confirm that fatty acid uptake and oxidation are targetable metabolic dependencies in human prostate cancer.
Implications:
Prostate cancer utilizes multiple substrates to fuel energy requirements, yet pharmacologic targeting of fatty acid uptake and oxidation reveals metabolic dependencies in localized and metastatic tumors.
Data from NEDD9 Regulates Actin Dynamics through Cortactin Deacetylation in an AURKA/HDAC6–Dependent Manner
Authors: Kozyreva Varvara K. , McLaughlin Sarah L. , Livengood Ryan H. , Calkins Robin A. , Kelley Laura C. , Rajulapati Anuradha , Ice Ryan J. , Smolkin Matthew B. , Weed Scott A. , Pugacheva Elena N.
Published: April 2023

Abstract
The prometastatic protein NEDD9 (neural precursor cell expressed, developmentally downregulated 9) is highly expressed in many cancers and is required for mesenchymal individual cell migration and progression to the invasive stage. Nevertheless, the molecular mechanisms of NEDD9-driven migration and the downstream targets effecting metastasis are not well defined. In the current study, knockdown of NEDD9 in highly metastatic tumor cells drastically reduces their migratory capacity due to disruption of actin dynamics at the leading edge. Specifically, NEDD9 deficiency leads to a decrease in the persistence and stability of lamellipodial protrusions similar to knockdown of cortactin (CTTN). Mechanistically, it was shown that NEDD9 binds to and regulates acetylation of CTTN in an Aurora A kinase (AURKA)/HDAC6–dependent manner. The knockdown of NEDD9 or AURKA results in an increase in the amount of acetylated CTTN and a decrease in the binding of CTTN to F-actin. Overexpression of the deacetylation mimicking (9KR) mutant of CTTN is sufficient to restore actin dynamics at the leading edge and migration proficiency of the tumor cells. Inhibition of AURKA and HDAC6 activity by alisertib and Tubastatin A in xenograft models of breast cancer leads to a decrease in the number of pulmonary metastases. Collectively, these findings identify CTTN as the key downstream component of NEDD9-driven migration and metastatic phenotypes.

Implications: This study provides a mechanistic platform for therapeutic interventions based on AURKA and HDAC6 inhibition for patients with metastatic breast cancer to prevent and/or eradicate metastases. Mol Cancer Res; 12(5); 681–93. ©2014 AACR.
Data from Biochemical and Structural Analysis of Common Cancer-Associated KRAS Mutations
Authors: Hunter John C. , Manandhar Anuj , Carrasco Martin A. , Gurbani Deepak , Gondi Sudershan , Westover Kenneth D.
Published: April 2023

Abstract
KRAS mutations are the most common genetic abnormalities in cancer, but the distribution of specific mutations across cancers and the differential responses of patients with specific KRAS mutations in therapeutic clinical trials suggest that different KRAS mutations have unique biochemical behaviors. To further explain these high-level clinical differences and to explore potential therapeutic strategies for specific KRAS isoforms, we characterized the most common KRAS mutants biochemically for substrate binding kinetics, intrinsic and GTPase-activating protein (GAP)–stimulated GTPase activities, and interactions with the RAS effector, RAF kinase. Of note, KRAS G13D shows rapid nucleotide exchange kinetics compared with other mutants analyzed. This property can be explained by changes in the electrostatic charge distribution of the active site induced by the G13D mutation as shown by X-ray crystallography. High-resolution X-ray structures are also provided for the GDP-bound forms of KRAS G12V, G12R, and Q61L and reveal additional insight. Overall, the structural data and measurements, obtained herein, indicate that measurable biochemical properties provide clues for identifying KRAS-driven tumors that preferentially signal through RAF.

Implications: Biochemical profiling and subclassification of KRAS-driven cancers will enable the rational selection of therapies targeting specific KRAS isoforms or specific RAS effectors. Mol Cancer Res; 13(9); 1325–35. ©2015 AACR.
Data from Notch3 Overexpression Promotes Anoikis Resistance in Epithelial Ovarian Cancer via Upregulation of COL4A2
Authors: Brown Caitlin W. , Brodsky Alexander S. , Freiman Richard N.
Published: April 2023

Abstract
Ovarian cancer is a lethal disease with the majority of diagnosed women having distant metastases. Interestingly, although Notch3 overexpression has been correlated with poor survival in epithelial ovarian cancer (EOC), little is known about its mechanism of action. Data show that Notch3 specifically promotes anoikis resistance. In addition, data indicate a positive role for focal adhesion kinase (FAK) as well as downstream signaling kinases such as Akt and Erk 1/2 in promoting anchorage-independent growth. Mechanistically, both mRNA transcript and protein levels of type IV collagen (COL4A2) are reduced when Notch3 levels are decreased and exogenous collagen IV supplementation reverses the anoikis sensitivity. Reduction of COL4A2 expression by RNAI-mediated knockdown induces cell death. Finally, elevated Notch3 expression levels correlate with higher COL4A2 expression in human ovarian tumor specimens.

Implications: These data highlight type IV collagen as a novel therapeutic target for metastatic EOC.

Visual Overview: http://mcr.aacrjournals.org/content/early/2014/11/25/1541-7786.MCR-14-0334/F1.large.jpg

Mol Cancer Res; 13(1); 78–85. ©2014 AACR.
Data from Altered Endosome Biogenesis in Prostate Cancer Has Biomarker Potential
Authors: Johnson Ian R.D. , Parkinson-Lawrence Emma J. , Shandala Tetyana , Weigert Roberto , Butler Lisa M. , Brooks Doug A.
Published: April 2023

Abstract
Prostate cancer is the second most common form of cancer in males, affecting one in eight men by the time they reach the age of 70 years. Current diagnostic tests for prostate cancer have significant problems with both false negatives and false positives, necessitating the search for new molecular markers. A recent investigation of endosomal and lysosomal proteins revealed that the critical process of endosomal biogenesis might be altered in prostate cancer. Here, a panel of endosomal markers was evaluated in prostate cancer and nonmalignant cells and a significant increase in gene and protein expression was found for early, but not late endosomal proteins. There was also a differential distribution of early endosomes, and altered endosomal traffic and signaling of the transferrin receptors (TFRC and TFR2) in prostate cancer cells. These findings support the concept that endosome biogenesis and function are altered in prostate cancer. Microarray analysis of a clinical cohort confirmed the altered endosomal gene expression observed in cultured prostate cancer cells. Furthermore, in prostate cancer patient tissue specimens, the early endosomal marker and adaptor protein APPL1 showed consistently altered basement membrane histology in the vicinity of tumors and concentrated staining within tumor masses. These novel observations on altered early endosome biogenesis provide a new avenue for prostate cancer biomarker investigation and suggest new methods for the early diagnosis and accurate prognosis of prostate cancer.

Implications: This discovery of altered endosome biogenesis in prostate cancer may lead to novel biomarkers for more precise cancer detection and patient prognosis. Mol Cancer Res; 12(12); 1851–62. ©2014 AACR.