bluuebunny/crossref_metadata_2025 huggingface dataset:
Changes in Retinal Structure and Ultrastructure in the Aged Mice Correlate With Differences in the Expression of Selected Retinal miRNAs
Authors: Hermenean Anca , Trotta Maria Consiglia , Gharbia Sami , Hermenean Andrei Gelu , Peteu Victor Eduard , Balta Cornel , Cotoraci Coralia , Gesualdo Carlo , Rossi Settimio , Gherghiceanu Mihaela , D’Amico Michele
Published: January 2021

Age and gender are two important factors that may influence the function and structure of the retina and its susceptibility to retinal diseases. The aim of this study was to delineate the influence that biological sex and age exert on the retinal structural and ultrastructural changes in mice and to identify the age-related miRNA dysregulation profiles in the retina by gender. Experiments were undertaken on male and female Balb/c aged 24 months (approximately 75–85 years in humans) compared to the control (3 months). The retinas were analyzed by histology, transmission electron microscopy, and age-related miRNA expression profile analysis. Retinas of both sexes showed a steady decline in retinal thickness as follows: photoreceptor (PS) and outer layers (p < 0.01 for the aged male vs. control; p < 0.05 for the aged female vs. control); the inner retinal layers were significantly affected by the aging process in the males (p < 0.01) but not in the aged females. Electron microscopy revealed more abnormalities which involve the retinal pigment epithelium (RPE) and Bruch’s membrane, outer and inner layers, vascular changes, deposits of amorphous materials, and accumulation of lipids or lipofuscins. Age-related miRNAs, miR-27a-3p (p < 0.01), miR-27b-3p (p < 0.05), and miR-20a-5p (p < 0.05) were significantly up-regulated in aged male mice compared to the controls, whereas miR-20b-5p was significantly down-regulated in aged male (p < 0.05) and female mice (p < 0.05) compared to the respective controls. miR-27a-3p (5.00 fold; p < 0.01) and miR-27b (7.58 fold; p < 0.01) were significantly up-regulated in aged male mice vs. aged female mice, whereas miR-20b-5p (−2.10 fold; p < 0.05) was significantly down-regulated in aged male mice vs. aged female mice. Interestingly, miR-27a-3p, miR-27b-3p, miR-20a-5p, and miR-20b-5p expressions significantly correlated with the thickness of the retinal PS layer (p < 0.01), retinal outer layers (p < 0.01), and Bruch’s membrane (p < 0.01). Our results showed that biological sex can influence the structure and function of the retina upon aging, suggesting that this difference may be underlined by the dysregulation of age-related mi-RNAs.
Hemokinin-1 as a Mediator of Arthritis-Related Pain via Direct Activation of Primary Sensory Neurons
Authors: Borbély Éva , Hunyady Ágnes , Pohóczky Krisztina , Payrits Maja , Botz Bálint , Mócsai Attila , Berger Alexandra , Szőke Éva , Helyes Zsuzsanna
Published: January 2021

The tachykinin hemokinin-1 (HK-1) is involved in immune cell development and inflammation, but little is known about its function in pain. It acts through the NK1 tachykinin receptor, but several effects are mediated by a yet unidentified target. Therefore, we investigated the role and mechanism of action of HK-1 in arthritis models of distinct mechanisms with special emphasis on pain. Arthritis was induced by i.p. K/BxN serum (passive transfer of inflammatory cytokines, autoantibodies), intra-articular mast cell tryptase or Complete Freund’s Adjuvant (CFA, active immunization) in wild type, HK-1- and NK1-deficient mice. Mechanical- and heat hyperalgesia determined by dynamic plantar esthesiometry and increasing temperature hot plate, respectively, swelling measured by plethysmometry or micrometry were significantly reduced in HK-1-deleted, but not NK1-deficient mice in all models. K/BxN serum-induced histopathological changes (day 14) were also decreased, but early myeloperoxidase activity detected by luminescent in vivo imaging increased in HK-1-deleted mice similarly to the CFA model. However, vasodilation and plasma protein extravasation determined by laser Speckle and fluorescent imaging, respectively, were not altered by HK-1 deficiency in any models. HK-1 induced Ca2+-influx in primary sensory neurons, which was also seen in NK1-deficient cells and after pertussis toxin-pretreatment, but not in extracellular Ca2+-free medium. These are the first results showing that HK-1 mediates arthritic pain and cellular, but not vascular inflammatory mechanisms, independently of NK1 activation. HK-1 activates primary sensory neurons presumably via Ca2+ channel-linked receptor. Identifying its target opens new directions to understand joint pain leading to novel therapeutic opportunities.
The Selective SIK2 Inhibitor ARN-3236 Produces Strong Antidepressant-Like Efficacy in Mice via the Hippocampal CRTC1-CREB-BDNF Pathway
Authors: Liu Yue , Tang Wenqian , Ji Chunhui , Gu Jianghong , Chen Yanmei , Huang Jie , Zhao Xinyi , Sun Yingfang , Wang Chengniu , Guan Wei , Liu Jianfeng , Jiang Bo
Published: January 2021

Depression is a widespread chronic medical illness affecting thoughts, mood, and physical health. However, the limited and delayed therapeutic efficacy of monoaminergic drugs has led to intensive research efforts to develop novel antidepressants. ARN-3236 is the first potent and selective inhibitor of salt-inducible kinase 2 (SIK2). In this study, a multidisciplinary approach was used to explore the antidepressant-like actions of ARN-3236 in mice. Chronic social defeat stress (CSDS) and chronic unpredictable mild stress (CUMS) models of depression, various behavioral tests, high performance liquid chromatography-tandem mass spectrometry, stereotactic infusion, viral-mediated gene transfer, western blotting, co-immunoprecipitation and immunofluorescence were used together. It was found that ARN-3236 could penetrate the blood-brain barrier. Repeated ARN-3236 administration induced significant antidepressant-like effects in both the CSDS and CUMS models of depression, accompanied with fully preventing the stress-enhanced SIK2 expression and cytoplasmic translocation of cyclic adenosine monophosphate response element binding protein (CREB)-regulated transcription coactivator 1 (CRTC1) in the hippocampus. ARN-3236 treatment also completely reversed the down-regulating effects of CSDS and CUMS on the hippocampal brain-derived neurotrophic factor (BDNF) system and neurogenesis. Moreover, we demonstrated that the hippocampal CRTC1-CREB-BDNF pathway mediated the antidepressant-like efficacy of ARN-3236. Collectively, ARN-3236 possesses strong protecting effects against chronic stress, and could be a novel antidepressant beyond monoaminergic drugs.
Efficient Numerical Solution of the EMI Model Representing the Extracellular Space (E), Cell Membrane (M) and Intracellular Space (I) of a Collection of Cardiac Cells
Authors: Jæger Karoline Horgmo , Hustad Kristian Gregorius , Cai Xing , Tveito Aslak
Published: January 2021

The EMI model represents excitable cells in a more accurate manner than traditional homogenized models at the price of increased computational complexity. The increased complexity of solving the EMI model stems from a significant increase in the number of computational nodes and from the form of the linear systems that need to be solved. Here, we will show that the latter problem can be solved by careful use of operator splitting of the spatially coupled equations. By using this method, the linear systems can be broken into sub-problems that are of the classical type of linear, elliptic boundary value problems. Therefore, the vast collection of methods for solving linear, elliptic partial differential equations can be used. We demonstrate that this enables us to solve the systems using shared-memory parallel computers. The computing time scales perfectly with the number of physical cells. For a collection of 512×256 cells, we solved linear systems with about2.5×108unknows. Since the computational effort scales linearly with the number of physical cells, we believe that larger computers can be used to simulate millions of excitable cells and thus allow careful analysis of physiological systems of great importance.
Confined Brownian Motion Tracked With Motion Blur: Estimating Diffusion Coefficient and Size of Confining Space
Authors: Mortensen Kim I. , Flyvbjerg Henrik , Pedersen Jonas N.
Published: January 2021

Mesoscopic environments and particles diffusing in them are often studied by tracking such particles individually while their Brownian motion explores their environment. Environments may be, e.g., a domain in a cell membrane, an interior compartment of a cell, or an engineered nanopit. Particle trajectories are typically determined from time-lapse recorded movies. These are recorded with sufficient exposure time per frame to be able to detect and localize particles in each frame. Since particles move during this exposure time, particles image with motion blur. This motion blur can compromise estimates of diffusion coefficients and the size of the confining domain if not accounted for correctly. We do that here. We give explicit and exact expressions for the variance of measured positions and the mean-squared displacement of a Brownian particle confined in, respectively, a 1D box, a 2D box, a 2D circular disc, and a 3D sphere. Our expressions are valid for all exposure times, irrespective of the size of the confining space and the value of the diffusion coefficient. They apply also in the common case where the exposure time is smaller than the time-lapse due, e.g., to “dead time” caused by the readout process in the camera. These expressions permit determination of diffusion coefficients and domain sizes for given movies for the simple geometries we consider. More important, the trends observed in our exact results when parameter values are varied are valid also for more complex geometries for which no exact analytical solutions exist. Wherever the underlying physics is the same, the exact quantitative description of its consequences provided here is portable as a qualitative and semi-quantitative understanding of its consequences in general. The results may also be useful for other types of reflected Brownian motion than those occurring in single-particle tracking, e.g., in nuclear magnetic resonance imaging techniques. For use in that particular context, we briefly discuss the effects of confinement on anisotropic Brownian motion imaged with motion blur.
A Peregrine Soliton-Like Structure That Has Nothing to Deal With a Peregrine Breather
Authors: Finot Christophe
Published: January 2021

We report on experimental results where a temporal intensity profile presenting some of the main signatures of the Peregrine soliton (PS) is observed. However, the emergence of a highly peaked structure over a continuous background in a normally dispersive fiber cannot be linked to any PS dynamics and is mainly ascribed to the impact of Brillouin backscattering.
Electromagnetic Metasurfaces and Reconfigurable Metasurfaces: A Review
Authors: Zahra Sidrish , Ma Liang , Wang Wenjiao , Li Jian , Chen Dexu , Liu Yifeng , Zhou Yuedan , Li Na , Huang Yongjun , Wen Guangjun
Published: January 2021

Metamaterial is a kind of material/structure that is artificially designed and has exceptional electromagnetic properties and/or other physical properties, not found in nature. A class of electromagnetic metamaterial with only one or a few layers of periodic or aperiodic arranged cell structures in the direction of electromagnetic waves propagation can be referred to as a metasurface. Metasurface can be considered as a two-dimensional representation of metamaterial and can realize the controlling of the amplitude, phase, and polarization state/direction of the incident electromagnetic wave. According to the novel electromagnetic characteristics of metasurface and its big advantages, a series of new planar devices and systems based on metasurface can be developed. The goal of this review article is firstly to provide introductions for basic metasurface, its significance properties, and application principles. Meanwhile, the main research progresses of regular metasurfaces and the newly developed reconfigurable metasurfaces are analyzed, focusing on the types of amplitude modulation, phase modulation, polarization modulation, and multidimensional modulation. Finally, the research significances of metasurface development trend and important engineering practical applications are analyzed in the end.
Layered CMOS SPADs for Low Noise Detection of Charged Particles
Authors: Ratti Lodovico , Brogi Paolo , Collazuol Gianmaria , Dalla Betta Gian-Franco , Marrocchesi Pier Simone , Pancheri Lucio , Sulay Arta , Torilla Gianmarco , Vacchi Carla
Published: January 2021

This paper reports the characterization of SPAD arrays fabricated in a 150 nm CMOS technology in view of applications to the detection of charged particles. The test vehicle contains SPADs with different active area and operated with different quenching techniques, either passive or active. The set of devices under test (DUTs) consists of single-tier chips, about 30 mm2 in area, with dual-tier structures where two chips are face-to-face bump bonded to each other. In the dual-layer structure obtained in this way, the coincidence signal between overlapping SPAD pairs is read out, with a beneficial impact on the dark count noise performance. The DUT characterization was mainly focused on studying the breakdown voltage in the single-layer arrays and the dark count rate (DCR), measured in different working conditions, in both the single- and the dual-layer structures. Comparison between the DCR performance of the two configurations clearly emphasizes the advantage of the coincidence readout architecture.
An Activated Potassium Phosphate Fertilizer Solution for Stimulating the Growth of Agricultural Plants
Authors: Belov Sergej V. , Danyleiko Yurij K. , Glinushkin Alexey P. , Kalinitchenko Valery P. , Egorov Alexey V. , Sidorov Vladimir A. , Konchekov Evgeny M. , Gudkov Sergey V. , Dorokhov Aleksey S. , Lobachevsky Yakov P. , Izmailov Andrey Yu
Published: January 2021

This study aimed to develop a sustainable industrial chemical engineering technology to improve the interaction between technology, plants, and soil in agriculture. The signaling crosstalk between H2O2 and NO and that between H2O2 and Ca2+ influence plant developmental and physiological processes. Many promising technologies for crop stimulation and protection are based on a thorough study of the environmental impact of various physical factors. A low-temperature, high-frequency plasma was generated via cathode high-frequency glow discharge and used for the electrolysis of an aqueous solution of a low concentration of the strong electrolyte KH2PO4, with an electrolysis-activated solution named Plasmolite. The Plasmolite solution yielded a Raman (red) scattering spectrum with a maximum at 1,640 cm−1, which was associated with hydrogen atom vibrations, and other bands at 875, 930, 1,050, and 1,123 cm−1, which were associated with the aqueous electrolyte solution plasma treatment. Based on the goal of producing an optimal H2O2 concentration of 100 μM, two types of seeds were exposed to a Plasmolite-based 2 × 10–5 M KH2PO4 solution moisturizing medium for three days. Approximately 92% of the spring spelled seeds (grade “Gremme”) that were exposed to this test solution sprouted, compared with 76% of the seeds exposed to a control solution. The spring rye seeds (grade “Onokhoyskaya”) that were exposed to the test solution sprouted at a rate of 90% compared with 75% in the control. The percentage of seeds that sprouted with a root length of more than 6 mm was approximately 80% for the test solution, compared to 50% for the control. Based on these results, the use of Plasmolite is considered to be promising for the production of activated H2O2 for protecting plants and stimulating growth, particularly for enhancing the functions of K and P2O5 of fertilizers.
Characterization of the Temporal Pattern of Blood Protein Digestion in Rhodnius prolixus: First Description of Early and Late Gut Cathepsins
Authors: Henriques Bianca Santos , Gomes Bruno , Oliveira Pedro Lagerblad , Garcia Elói de Souza , Azambuja Patrícia , Genta Fernando Ariel
Published: January 2021

Rhodnius prolixus is one important vector for the parasite Trypanosoma cruzi in Latin America, where Chagas disease is a significant health issue. Although R. prolixus is a model for investigations of vector–parasite interaction and transmission, not much has been done recently to further comprehend its protein digestion. In this work, gut proteolysis was characterized using new fluorogenic substrates, including optimum pH, inhibition profiles, and tissue and temporal expression patterns. Each protease possessed a particular tissue prevalence and activity cycle after feeding. Cathepsin L had a higher activity in the posterior midgut lumen, being characterized by a plateau of high activities during several days in the intermediate phase of digestion. Cathepsin D showed high activity levels in the tissue homogenates and in the luminal content of the posterior midgut, with a single peak 5 days after blood feeding. Aminopeptidases are highly associated with the midgut wall, where the highest activity is located. Assays with proteinaceous substrates as casein, hemoglobin, and serum albumin revealed different activity profiles, with some evidence of biphasic temporal proteolytic patterns. Cathepsin D genes are preferentially expressed in the anterior midgut, while cathepsin L genes are mainly located in the posterior portion of the midgut, with specific sets of genes being differently expressed in the initial, intermediate, or late phases of blood digestion.

Significance Statement

This is the first description in a non-dipteran hematophagous species of a sequential protease secretion system based on midgut cathepsins instead of the most common insect digestive serine proteases (trypsins and chymotrypsins). The midgut of R. prolixus (Hemiptera) shows a different temporal expression of proteases in the initial, intermediate, and late stages of blood digestion. In this respect, a different timing in protease secretion may be an example of adaptative convergence in blood-sucking vectors from different orders. Expanding the knowledge about gut physiology in triatomine vectors may contribute to the development of new control strategies, aiming the blocking of parasite transmission.
Melatonin Lowers HIF-1α Content in Human Proximal Tubular Cells (HK-2) Due to Preventing Its Deacetylation by Sirtuin 1
Authors: Owczarek Aleksandra , Gieczewska Katarzyna B. , Polanska Marta , Paterczyk Bohdan , Gruza Andrzej , Winiarska Katarzyna
Published: January 2021

Although melatonin is widely known for its nephroprotective properties, there are no reports clearly pointing at its impact on the activity of hypoxia-inducible factor-1 (HIF-1), the main mediator of metabolic responses to hypoxia, in kidneys. The aim of the present study was to elucidate how melatonin affects the expression of the regulatory subunit HIF-1α in renal proximal tubules. HK-2 cells, immortalized human proximal tubular cells, were cultured under hypoxic conditions (1% O2). Melatonin was applied at 100 μM concentration. Protein and mRNA contents were determined by Western blot and RT-qPCR, respectively. HIF-1α acetylation level was established by means of immunoprecipitation followed by Western blot. Melatonin receptors MT1 and MT2 localization in HK-2 cells was visualized using immunofluorescence confocal analysis. It was found that melatonin in HK-2 cells (1) lowered HIF-1α protein, but not mRNA, content; (2) attenuated expression of HIF-1 target genes; (3) increased HIF-1α acetylation level; and (4) diminished sirtuin 1 expression (both protein and mRNA). Sirtuin 1 involvement in the regulation of HIF-1α level was confirmed applying cells with silenced Sirt1 gene. Moreover, the presence of membrane MT1 and MT2 receptors was identified in HK-2 cells and their ligand, ramelteon, turned out to mimic melatonin action on both HIF-1α and sirtuin 1 levels. Thus, it is concluded that the mechanism of melatonin-evoked decline in HIF-1α content in renal proximal tubular cells involves increased acetylation of this subunit which results from the attenuated expression of sirtuin 1, an enzyme reported to deacetylate HIF-1α. This observation provides a new insight to the understanding of melatonin action in kidneys.
High Throughput Sequencing of MicroRNA in Rainbow Trout Plasma, Mucus, and Surrounding Water Following Acute Stress
Authors: Ikert Heather , Lynch Michael D. J. , Doxey Andrew C. , Giesy John P. , Servos Mark R. , Katzenback Barbara A. , Craig Paul M.
Published: January 2021

Circulating plasma microRNAs (miRNAs) are well established as biomarkers of several diseases in humans and have recently been used as indicators of environmental exposures in fish. However, the role of plasma miRNAs in regulating acute stress responses in fish is largely unknown. Tissue and plasma miRNAs have recently been associated with excreted miRNAs; however, external miRNAs have never been measured in fish. The objective of this study was to identify the altered plasma miRNAs in response to acute stress in rainbow trout (Oncorhynchus mykiss), as well as altered miRNAs in fish epidermal mucus and the surrounding ambient water. Small RNA was extracted and sequenced from plasma, mucus, and water collected from rainbow trout pre- and 1 h-post a 3-min air stressor. Following small RNA-Seq and pathway analysis, we identified differentially expressed plasma miRNAs that targeted biosynthetic, degradation, and metabolic pathways. We successfully isolated miRNA from trout mucus and the surrounding water and detected differences in miRNA expression 1-h post air stress. The expressed miRNA profiles in mucus and water were different from the altered plasma miRNA profile, which indicated that the plasma miRNA response was not associated with or immediately reflected in external samples, which was further validated through qPCR. This research expands understanding of the role of plasma miRNA in the acute stress response of fish and is the first report of successful isolation and profiling of miRNA from fish mucus or samples of ambient water. Measurements of miRNA from plasma, mucus, or water can be further studied and have potential to be applied as non-lethal indicators of acute stress in fish.
Quantifying Dynamical High-Order Interdependencies From the O-Information: An Application to Neural Spiking Dynamics
Authors: Stramaglia Sebastiano , Scagliarini Tomas , Daniels Bryan C. , Marinazzo Daniele
Published: January 2021

We address the problem of efficiently and informatively quantifying how multiplets of variables carry information about the future of the dynamical system they belong to. In particular we want to identify groups of variables carrying redundant or synergistic information, and track how the size and the composition of these multiplets changes as the collective behavior of the system evolves. In order to afford a parsimonious expansion of shared information, and at the same time control for lagged interactions and common effect, we develop a dynamical, conditioned version of the O-information, a framework recently proposed to quantify high-order interdependencies via multivariate extension of the mutual information. The dynamic O-information, here introduced, allows to separate multiplets of variables which influence synergistically the future of the system from redundant multiplets. We apply this framework to a dataset of spiking neurons from a monkey performing a perceptual discrimination task. The method identifies synergistic multiplets that include neurons previously categorized as containing little relevant information individually.
Adult and Developing Zebrafish as Suitable Models for Cardiac Electrophysiology and Pathology in Research and Industry
Authors: Echeazarra Leyre , Hortigón-Vinagre Maria Pura , Casis Oscar , Gallego Mónica
Published: January 2021

The electrophysiological behavior of the zebrafish heart is very similar to that of the human heart. In fact, most of the genes that codify the channels and regulatory proteins required for human cardiac function have their orthologs in the zebrafish. The high fecundity, small size, and easy handling make the zebrafish embryos/larvae an interesting candidate to perform whole animal experiments within a plate, offering a reliable and low-cost alternative to replace rodents and larger mammals for the study of cardiac physiology and pathology. The employment of zebrafish embryos/larvae has widened from basic science to industry, being of particular interest for pharmacology studies, since the zebrafish embryo/larva is able to recapitulate a complete and integrated view of cardiac physiology, missed in cell culture. As in the human heart, IKris the dominant repolarizing current and it is functional as early as 48 h post fertilization. Finally, genome editing techniques such as CRISPR/Cas9 facilitate the humanization of zebrafish embryos/larvae. These techniques allow one to replace zebrafish genes by their human orthologs, making humanized zebrafish embryos/larvae the most promisingin vitromodel, since it allows the recreation of human-organ-like environment, which is especially necessary in cardiac studies due to the implication of dynamic factors, electrical communication, and the paracrine signals in cardiac function.
Circadian Mechanisms: Cardiac Ion Channel Remodeling and Arrhythmias
Authors: Bernardi Joyce , Aromolaran Kelly A. , Zhu Hua , Aromolaran Ademuyiwa S.
Published: January 2021

Circadian rhythms are involved in many physiological and pathological processes in different tissues, including the heart. Circadian rhythms play a critical role in adverse cardiac function with implications for heart failure and sudden cardiac death, highlighting a significant contribution of circadian mechanisms to normal sinus rhythm in health and disease. Cardiac arrhythmias are a leading cause of morbidity and mortality in patients with heart failure and likely cause ∼250,000 deaths annually in the United States alone; however, the molecular mechanisms are poorly understood. This suggests the need to improve our current understanding of the underlying molecular mechanisms that increase vulnerability to arrhythmias. Obesity and its associated pathologies, including diabetes, have emerged as dangerous disease conditions that predispose to adverse cardiac electrical remodeling leading to fatal arrhythmias. The increasing epidemic of obesity and diabetes suggests vulnerability to arrhythmias will remain high in patients. An important objective would be to identify novel and unappreciated cellular mechanisms or signaling pathways that modulate obesity and/or diabetes. In this review we discuss circadian rhythms control of metabolic and environmental cues, cardiac ion channels, and mechanisms that predispose to supraventricular and ventricular arrhythmias including hormonal signaling and the autonomic nervous system, and how understanding their functional interplay may help to inform the development and optimization of effective clinical and therapeutic interventions with implications for chronotherapy.
LED Lighting Strategies Affect Physiology and Resilience to Pathogens and Pests in Eggplant (Solanum melongena L.)
Authors: Anja Dieleman J. , Marjolein Kruidhof H. , Weerheim Kees , Leiss Kirsten
Published: January 2021

Over the last decade, LED lighting has gained considerable interest as an energy-efficient supplemental light source in greenhouse horticulture that can change rapidly in intensity and spectral composition. Spectral composition not only affects crop physiology but may also affect the biology of pathogens, pests, and their natural enemies, both directly and indirectly through an impact on induced plant resistance. In this study, we investigated the effects of light spectrum against a background of sunlight on growth and development of Solanum melongena. These effects were related to the spectral effects on the establishment of populations of the predatory mite Amblyseius swirskii and plant resilience against the biotrophic fungus powdery mildew, the necrotrophic fungus botrytis, and the herbivorous arthropod Western flower thrips. The effects of a reduced red/far-red (R:FR) ratio were studied under two ratios of red to blue light. Far-red light either was supplied additionally to the photosynthetic photon flux density (PPFD) or partially replaced PPFD, while maintaining total photon flux density (PFD). Effects of white light or additional UV-B light on plant resilience was tested, compared to the reference (5% blue, 5% green, and 90% red light). Plant biomass in the vegetative phase increased when additional far-red light was supplied. Stem length increased with far-red, irrespective of PPFD and the percentage of blue light. In the generative phase, total shoot biomass and fruit fresh weights were higher under additional far-red light, followed by the treatments where far-red partly replaced PPFD. Far-red light increased biomass partitioning into the fruits, at the expense of the leaves. There were no differences in population growth of A. swirskii mites between light treatments, nor did light treatment have an effect on the vertical distribution of these predatory mites in the plants. The treatments with additional far-red light reduced the infection rate of powdery mildew, but increased botrytis infection. These differences might be due to the plant defenses acting against these pathogens evolving from two different regulatory pathways. These results show that positive effects of altered spectral compositions on physiological responses were only moderately compensated by increased susceptibility to fungal pathogens, which offers perspective for a sustainable greenhouse horticulture.
Engineering Alfalfa to Produce 2-O-Caffeoyl-L-Malate (Phaselic Acid) for Preventing Post-harvest Protein Loss via Oxidation by Polyphenol Oxidase
Authors: Sullivan Michael L. , Green Heather A. , Verdonk Julian C.
Published: January 2021

Many plants accumulate high levels of hydroxycinnamoyl esters and amides in their tissues, presumably to protect against biotic and abiotic stress. Red clover (Trifolium pretense) leaves accumulate high levels [5–15 mmol/kg fresh weight (FW)] of caffeic acid derivatives, including phaselic acid (2-O-caffeoyl-L-malate). Oxidation of caffeoyl-malate by an endogenous polyphenol oxidase (PPO) has been shown to help preserve forage protein after harvest and during storage as silage, which should improve N use efficiency in dairy and other ruminant production systems. The widely grown forage alfalfa lacks both PPO and PPO substrates and experiences substantial loss of protein following harvest. We previously identified a hydroxycinnamoyl-coenzyme A (CoA):malate hydroxycinnamoyl transferase (HMT, previously called HCT2) responsible for phaselic accumulation in red clover. With the goal of producing PPO-oxidizable compounds in alfalfa to help preserve forage protein, we expressed red clover HMT in alfalfa. Leaves of these alfalfa accumulated mainly p-coumaroyl- and feruloyl-malate (up to 1.26 and 0.25 mmol/kg FW, respectively). Leaves of HMT-expressing alfalfa supertransformed with an RNA interference (RNAi) construct to silence endogenous caffeoyl-CoA acid O-methyltransferase (CCOMT) accumulated high levels of caffeoyl-malate, as well as the p-coumaroyl and feruloyl esters (up to 2.16, 2.08, and 3.13 mmol/kg FW, respectively). Even higher levels of caffeoyl- and p-coumaroyl-malate were seen in stems (up to 8.37 and 3.15 mmol/kg FW, respectively). This level of caffeoyl-malate accumulation was sufficient to inhibit proteolysis in a PPO-dependent manner in in vitro experiments, indicating that the PPO system of post-harvest protein protection can be successfully adapted to alfalfa.
Referential vs. Non-referential World-Language Relations: How Do They Modulate Language Comprehension in 4 to 5-Year-Olds, Younger, and Older Adults?
Authors: Maquate Katja , Knoeferle Pia
Published: January 2021

Age has been shown to influence language comprehension, with delays, for instance, in older adults' expectations about upcoming information. We examined to what extent expectations about upcoming event information (who-does-what-to-whom) change across the lifespan (in 4- to 5-year-old children, younger, and older adults) and as a function of different world-language relations. In a visual-world paradigm, participants in all three age groups inspected a speaker whose facial expression was either smiling or sad. Next they inspected two clipart agents (e.g., a smiling cat and a grumpy rat) depicted as acting upon a patient (e.g., a ladybug tickled by the cat and arrested by the rat). Control scenes featured the same three characters without the action depictions. While inspecting the depictions, comprehenders listened to a German sentence [e.g., Den Marienkäfer kitzelt vergnügt der Kater; literally: “The ladybug (object/patient) tickles happily the cat (subject/agent)”]. Referential verb-action relations (i.e., when the actions were present) could, in principle, cue the cat-agent and so could non-referential relations via links from the speaker's smile to “happily” and the cat's smile. We examined variation in participants' visual anticipation of the agent (the cat) before it was mentioned depending on (a) participant age and (b) whether the referentially mediated action depiction or the non-referentially associated speaker smile cued the agent. The action depictions rapidly boosted participants' visual anticipation of the agent, facilitating thematic role assignment in all age groups. By contrast, effects of the non-referentially cued speaker smile emerged in the younger adults only. We outline implications of these findings for processing accounts of the temporally coordinated interplay between listeners' age-dependent language comprehension, their interrogation of the visual context, and visual context influences.
Asomatognosia: Structured Interview and Assessment of Visuomotor Imagery
Authors: Saetta Gianluca , Zindel-Geisseler Olivia , Stauffacher Franziska , Serra Carlo , Vannuscorps Gilles , Brugger Peter
Published: January 2021

Asomatognosia designates the experience that one’s body has faded from awareness. It is typically a somaesthetic experience but may target the visual modality (“asomatoscopy”). Frequently associated symptoms are the loss of ownership or agency over a limb. Here, we elaborate on the rigorous nosographic classification of asomatognosia and introduce a structured interview to capture both its core symptoms and associated signs of bodily estrangement. We additionally report the case of a pure left-sided hemiasomatognosia occurring after surgical removal of a meningioma in the right atrium. Despite the wide lesions of the right angular gyrus and of the temporo-parietal junction, the patient did not present visuospatial deficits or bodily awareness disorders other than hemiasomatognosia. The patient and 10 matched controls’ motor imagery was formally assessed with a limb laterality task in which they had to decide whether hands and feet presented under different angles of rotation depicted a left or a right limb. Bayesian statistics showed that patient’s reaction times were significantly impaired exclusively for the left foot and especially for mental rotations requiring somatomotor rather than visual limb representations. This was in accordance with a more enduring left-sided hemiasomatognosia for the lower limbs confined to the somesthetic modality. Our findings shed new light on motor imagery in asomatognosia and encourage the future use of the structured interview introduced here. In addition, the limb laterality task may capture phenomenological elements of a case by chronometric means. This allows a more standardized reporting of phenomenological detail and improves communication across different clinical facilities.
Explaining Positional Differences of Performance Profiles for the Elite Female Basketball Players
Authors: Zhai Zongpeng , Guo Yongbo , Zhang Shaoliang , Li Yuanchang , Liu Hongyou
Published: January 2021

The aim of the present study was to explore the differences in technical performances of players considering playing positions by controlling the effect of situational variables in each FIBA female continental basketball competition. Samples of 9,208 observations from 471 games in the America, Africa, Asia, and Europe Championships during 2013–2017 were collected and analyzed by generalized mixed linear modeling. The results showed that Centers from Europe had more 2-point made (ES = 0.69), 2-point attempted (ES = 0.79), and offensive (ES = 0.64) and defensive (ES = 0.48) rebounds than forward. Asian and European guards performed a fewer number of 2-point made (ES = 0.90; 0.91), 2-point attempted (ES = 1.06; 0.98), and offensive (ES = 1.30; 1.23) and defensive (ES = 0.93; 0.94) rebounds than Asian and European centers. African and Asian forward had more 2-point made (ES = 0.48; 0.50), 2-point attempted (ES = 0.50; 0.56) than guards. This study helps to better understand the technical demands of female basketball among different international competitions, which could pave a new way to analyze the development trend of female basketball and promoting specific training plans and game strategies for coaches and players.
Effect of Modulating DLPFC Activity on Antisocial and Prosocial Behavior: Evidence From a tDCS Study
Authors: Zheng Wanjun , Li Yuzhen , Ye Hang , Luo Jun
Published: January 2021

Antisocial behavior and prosocial behavior in the condition of inequality have long been observed in daily life. Understanding the neurological mechanisms and brain regions associated with antisocial and prosocial behavior and the development of new interventions are important for reducing violence and inequality. Fortunately, neurocognitive research and brain imaging research have found a correlation between antisocial or prosocial behavior and the prefrontal cortex. Recent brain stimulation research adopting transcranial direct current stimulation or transcranial magnetic stimulation has shown a causal relationship between brain regions and behaviors, but the findings are mixed. In the present study, we aimed to study whether stimulation of the DLPFC can change participants’ antisocial and prosocial behavior in the condition of inequality. We integrated antisocial and prosocial behavior in a unified paradigm. Based on this paradigm, we discussed costly and cost-free antisocial and prosocial behavior. In addition, we also measured participants’ disadvantageous and advantageous inequality aversion. The current study revealed an asymmetric effect of bilateral stimulation over the DLPFC on costly antisocial behavior, while such an effect of antisocial behavior without cost and prosocial behavior with and without cost were not observed. Moreover, costly antisocial behavior exhibited by men increased after receiving right anodal/left cathodal stimulation and decreased after receiving right cathodal anodal/left anodal stimulation compared with the behavior observed under sham stimulation. However, subjects’ inequality aversion was not influenced by tDCS.
Benefits and Challenges of Interdisciplinarity in CSCL Research: A View From the Literature
Authors: Hmelo-Silver Cindy E. , Jeong Heisawn
Published: January 2021

Computer-supported collaborative learning (CSCL) has a history of being interdisciplinary from its conception. Its beginnings have included computer scientists, psychologists, cognitive scientists, and educational researchers. These collaborations have been fruitful but have also posed challenges (Suthers et al., 2013). This article builds on the authors’ extensive review of the CSCL literature to examine the nature of interdisciplinary collaboration in CSCL research as well as an interdisciplinary CSCL workshop. Using a corpus of more than 700 CSCL articles, we reported an updated analysis for the theories and methods used in CSCL research. In addition, bibliometric analyses examined journals that publish CSCL research and are cited by CSCL research. CSCL research is published in journals that are aligned with interdisciplinary research with large contributions from educational research followed by technology related fields and social sciences. The contributions from domain knowledge journals are relatively weak. These analyses revealed disciplinary influences and uptakes of CSCL research and how they might differ across CSCL research clusters. Lastly, we provide a case example of a CSCL workshop to further demonstrate the interdisciplinary nature of the field. Through these analyses we aim to characterize the benefits and challenges of interdisciplinary collaboration in CSCL research. Interdisciplinarity has helped CSCL research to adopt multiple theories and methods to understand CSCL. While cultivating diversity, we also need to be mindful that research outcomes are exchanged and appropriated actively across participating disciplines so that our understanding of CSCL rises above individual disciplines.
The Effects of Working Memory Updating Training in Parkinson’s Disease: A Feasibility and Single-Subject Study on Cognition, Movement and Functional Brain Response
Authors: Walton Lois , Domellöf Magdalena Eriksson , Boraxbekk Carl-Johan , Domellöf Erik , Rönnqvist Louise , Bäckström David , Forsgren Lars , Stigsdotter Neely Anna
Published: January 2021

In Parkinson’s disease (PD), the fronto-striatal network is involved in motor and cognitive symptoms. Working memory (WM) updating training engages this network in healthy populations, as observed by improved cognitive performance and increased striatal BOLD signal. This two-part study aimed to assess the feasibility of WM updating training in PD and measure change in cognition, movement and functional brain response in one individual with PD after WM updating training. A feasibility and single-subject (FL) study were performed in which patients with PD completed computerized WM updating training. The outcome measures were the pre-post changes in criterion and transfer cognitive tests; cognitive complaints; psychological health; movement kinematics; and task-related BOLD signal. Participants in the feasibility study showed improvements on the criterion tests at post-test. FL displayed the largest improvements on the criterion tests and smaller improvements on transfer tests. Furthermore, FL reported improved cognitive performance in everyday life. A shorter onset latency and smoother upper-limb goal-directed movements were measured at post-test, as well as increased activation within the striatum and decreased activation throughout the fronto-parietal WM network. This two-part study demonstrated that WM updating training is feasible to complete for PD patients and that change occurred in FL at post-test in the domains of cognition, movement and functional brain response.
The Contribution of Common and Specific Therapeutic Factors to Mindfulness-Based Intervention Outcomes
Authors: Canby Nicholas K. , Eichel Kristina , Lindahl Jared , Chau Sathiarith , Cordova James , Britton Willoughby B.
Published: January 2021

While Mindfulness-Based Interventions (MBIs) have been shown to be effective for a range of patient populations and outcomes, a question remains as to the role of common therapeutic factors, as opposed to the specific effects of mindfulness practice, in contributing to patient improvements. This project used a mixed-method design to investigate the contribution of specific (mindfulness practice-related) and common (instructor and group related) therapeutic factors to client improvements within an MBI. Participants with mild-severe depression (N= 104; 73% female,Mage = 40.28) participated in an 8-week MBI. Specific therapeutic factors (formal out-of-class meditation minutes and informal mindfulness practice frequency) and social common factors (instructor and group ratings) were entered into multilevel growth curve models to predict changes in depression, anxiety, stress, and mindfulness at six timepoints from baseline to 3-month follow-up. Qualitative interviews with participants provided rich descriptions of how instructor and group related factors played a role in therapeutic trajectories. Findings indicated that instructor ratings predicted changes in depression and stress, group ratings predicted changes in stress and self-reported mindfulness, and formal meditation predicted changes in anxiety and stress, while informal mindfulness practice did not predict client improvements. Social common factors were stronger predictors of improvements in depression, stress, and self-reported mindfulness than specific mindfulness practice-related factors. Qualitative data supported the importance of relationships with instructor and group members, involving bonding, expressing feelings, and instilling hope. Our findings dispel the myth that MBI outcomes are exclusively the result of mindfulness meditation practice, and suggest that social common factors may account for much of the effects of these interventions. Further research on meditation should take into consideration the effects of social context and other common therapeutic factors.
Unmute the Organization Through Serious Play
Authors: Heldal Frode , Dehlin Erlend , Oddane Torild Alise
Published: January 2021

In this article, we sketch up an action research process designed to give voice to those who traditionally have not had a voice in organizations. In particular, the research process was structured around “serious play” and designed as a talk show, where researchers played parts, including a talk show host, and where questions pertaining to organizational life were discussed in depth. The structure of the discussion was construed based on reflective teams, i.e., two actors performing a dialogue (talk show host and guest) and a silent group (audience) as listeners. The key research question concerns in what ways such an action research process is replicable? Applying a critical lens, we argue that even if strong claims of replicability are not met, as in being able to reproduce results and/or generalize them, this is outside the point. Rather, as we set out to apply a qualitative research design to achieve cogenerative learning effects, we advance an understanding of replicability-as-recoverability. This entails giving explicit grounds for our epistemic anchoring in critical realism and sketching out a research design which is sufficiently clear and transparent to undergo critical scrutiny.