bluuebunny/crossref_metadata_2025 huggingface dataset:
Prognostic factors and overall survival among patients with ovarian cancer in the pre-PARP inhibitor era: the OCRWE-Finland study
Authors: Lahelma Mari , Rauhamaa Heini , Leskelä Riikka-Leena , Isomeri Outi , Idänpään-Heikkilä Juhana , Käkelä Sari , Roebuck Nichola , Mascialino Barbara , Hietanen Sakari , Loukovaara Mikko , Auranen Annika
Published: October 2024

Background: Despite recent treatment advances in ovarian cancer (OC), more real-world evidence studies investigating patient outcomes are needed. OCRWE-Finland was an observational cohort study investigating OC outcomes in Finland during the pre-PARP inhibitor era. Patients: Patients were diagnosed with OC between 2014 and 2019 in Finland. This analysis reports baseline characteristics of all patients, patients with high-grade serous OC (HGSOC), and overall survival (OS) for patients with HGSOC. Results: Among 1,711 patients diagnosed with OC, 867 (51%) had HGSOC. The absence versus presence of visible residual disease post-debulking surgery was associated with improved OS for patients at stage III (n = 303; median: NR vs. 43 months; p = 0.005), but not stage IV (n = 118; median: 37 months vs. 40 months; p = 0.96). Bevacizumab treatment at any line at stages III/IV improved OS in the short-term only. Receiving versus not receiving bevacizumab at first-line for patients with visible residual disease post-debulking surgery was associated with improved OS at stage III (median: 48 months vs. 36 months; p = 0.003), but not stage IV (median: 42 months vs. 37 months; p = 0.26). Multivariate Cox regression analyses showed that stage IV at initial diagnosis and the presence of R2 classification post-debulking surgery resulted in poorer OS. Interpretation: In the pre-PARP inhibitor era, the absence versus presence of visible residual disease post-debulking surgery was associated with improved OS in stage III, but not stage IV HGSOC. First-line bevacizumab seemed to be beneficial in patients with stage III HGSOC and visible residual disease.
Real-life treatment patterns and time to next treatment among patients with ovarian cancer in the pre-PARP inhibitor era: the OCRWE-Finland Study
Authors: Lahelma Mari , Rauhamaa Heini , Isomeri Outi , Idänpään-Heikkilä Juhana , Käkelä Sari , Roebuck Nichola , Mascialino Barbara , Hietanen Sakari , Loukovaara Mikko , Auranen Annika
Published: October 2024

Background: As the treatment landscape for advanced ovarian cancer (OC) evolves, it is important to understand patient outcomes in real-world clinical practice. OCRWE-Finland was an observational cohort study investigating OC outcomes, including treatment patterns, time to next treatment 1 (TTNT1), overall survival and healthcare resource utilisation, in Finland during the pre-PARPi era.
Materials and methods: Patients included in OCRWE-Finland were diagnosed with OC between 2014 and 2019. Here, we report treatment patterns and TTNT1 outcomes (as a surrogate for progression-free survival) for patients in the high-grade serous ovarian carcinoma (HGSOC) cohort.
Results: In OCRWE-Finland, there were 867 patients with HGSOC. Of the 811 patients who received first-line treatment, the most common regimen was surgery and adjuvant chemotherapy (53%), and 227 patients also received first-line bevacizumab. Median TTNT1 among 623 patients with stage III/IV disease was 19 months (95% confidence interval, 18–21 months), with no difference between patients with stage III or IV disease (p = 0.24). The presence versus absence of visible residual disease post-debulking surgery was associated with shorter TTNT1 among patients with stage III tumours (p = 0.031) but showed no impact for stage IV tumours (p = 0.55). First-line versus no first-line bevacizumab was associated with shorter TTNT1 among stages I–IV (p < 0.0001) but did not affect patients with stage III/IV tumours (p = 0.45).
Interpretation: In the pre-PARPi era, prognosis for advanced OC was poor, particularly for patients with stage III tumours and visible residual disease or stage IV tumours regardless of the presence of residual disease. The increasing use of PARPis will hopefully help address the need for effective treatments in advanced OC.
Research on the behavior of molten pool in laser deep-penetration welding
Authors: Yang Maohong , Cai Detao , Gao Shiyi , Fang Weiping , Luo Ziyi , Huang Jiankang , Yevhenii Illiashenko , Shevchenko Vitalii
Published: October 2024

This paper mainly studies the behavior of the laser deep-penetration welding melt pool. A laser deep-penetration welding melt pool model under adaptive combined heat sources was established using numerical simulation software. This model can automatically find the keyhole radius and keyhole bottom during the welding process so that all the heat source energy acts on the keyhole wall. The results show that deep and narrow keyholes are more prone to bubble formation; the free surface descends, and the melt pool flow velocity slows down as the keyhole becomes shallower, eventually resulting in a wavy weld seam; once a hump forms on the keyhole wall, there is a spontaneous tendency for the keyhole to close under the action of forces in the melt pool.
Myogenic satellite cells are a cambial reserve of muscle tissue
Authors: Odintsova I. A , Chepurnenko M. N , Komarova A. S
Published: October 2024

The review deals with cell sources of histogenesis and regeneration of striated skeletal muscle tissue. Heterogeneity of cambial cells of this tissue as well as an important role of their microenvironment to provide their activation, proliferation, differentiation and specialization is displayed. Issues of myogenic satellite cells cytogenesis within the embryonic and postnatal development are discussed. The data on molecular markers of myosatellitocytes and some other tissue components are provided. Issues of non-myogenic sources of skeletal muscle tissue histogenesis are discussed. The significance of studying a cambial cell reserve for the development of effective treatment modalities of muscular dystrophy is emphasized. An experimental material should be analyzed with consideration of the fundamental theoretical concepts on the regularities of histogenesis, cell differons, the theory of stem cell.
Optimization of conditions of skin and gingival mucosa derived human fibroblasts obtainment and cultivation
Authors: Zorin V. L , Kopnin P. B , Zorina A. I , Eremin I. I , Lazareva N. L , Chauzova T. S , Samchuk D. P , Petrikina A. P , Eremin P. S , Korsakov I. N , Grinakovskaya O. S , Solovieva E. V , Kotenko K. V , Pulin A. A
Published: October 2024

For optimization of conditions of skin and gingival mucosa derived human fibroblasts obtainment and cultivation in this work we performed comparative analysis of 11 fetal bovine serums (from different manufacturers) and autologous human serum. Our analysis included also comparison of 3 serum-free media and 4 serum-reduced media. Evaluation criterion at the step of primary cell culture was colony-formation efficiency and during subsequent cultivation - fibroblasts population doubling time. Results revealed that ability of skin-derived fibroblasts to form colonies could vary 4 and more times and gingival mucosa derived fibroblasts - 1,5-1,8 times depending on used for cultivation type of serum or medium. Clonal analysis showed that fibroblasts in culture form 3 types of colonies consisting of different amount of cells and different phenotype. Ratio of colonies could be variable and depend on type of used medium. It was noticed during cultivation that addition of different sera to standard medium (DMEM) led to changing of cell's doubling time. In general, successful obtainment of fibroblasts primary culture determined by selection of appropriate serum and medium providing development of large colonies of rapidly proliferating cells. It was noticed during cultivation that addition of different sera to standard medium (DMEM) led to changes in cell's doubling time.
Experimental study of autologous multipotent mesenchymal stromal cells in hepatic cirrhosis treatment
Authors: Agaev B. A , Agaev R. M , Popandopulo A. G , Djafarli R. E
Published: October 2024

Cirrhosis of the liver is a serious disease characterized by replacement of liver parenchyma by fibrous tissue. Standard conservative treatments are not effective. In this regard, it's actual to develop the new approaches, the most effective of which may be turned out the cellular technologies. In this study we evaluated the efficacy of multipotent mesenchymal stromal cells (MMSC) introduced via the common hepatic artery or portal vein in the treatment of experimentally induced liver cirrhosis in rats. According to biochemical, ultrasound and histological methods, MMSC were shown to be effective in liver cirrhosis. Interestingly, the more pronounced positive dynamics was observed with intraarterial administration of cells.
Cholinesterases: the opinion of neurophysiologist
Authors: Petrov K. A , Kharlamova A. D , Nikolsky E. E
Published: October 2024

The review addresses issues of structure and functions of acetyl- and butyrylcholinesterases. Authors consider these enzymes not only as limiters of the neurotransmitter acetylcholine life span in synaptic cleft but also accounting for their putative non-synaptic functions. Particular emphasis has been placed on the possibility of correction of nerve system pathologies by way of modification of the activity of these enzymes.
Morphologic assessment of compensatory abilities of scalp skin cells in staged dermotensia
Authors: Checmareva I. A , Paklina O. V , Sharobaro V. I , Vaganova N. A , Gordienko E. N , Vaganov N. V , Grechishnikov M. I
Published: October 2024

The purpose of the research was morphologic (histologic and electron-microscopic) study of compensatory adaptational reaction of skin of the scalp after staged expansion. Morphologic assessment of adequacy of the expansion and compensatory abilities of cells of tissues under expansion was carried out in 27 patients. It was ascertained in the course of the work that the secondary dermotensia (twofold, threefold, fourfold) did not provoke degenerative changes in expanded scalp tissues, ultrastructural changes of cells of epidermis and dermis characterized adaptable reactions that did not exceed their compensatory abilities.
Perspectives of using stem cells in diabetes mellitus treatment
Authors: Plushkina A. S , Kaligin M. S
Published: October 2024

Using stem cells is one of the most perspective methods of diabetes mellitus treatment. Different stem cells populations are used for this purpose. Pancreatic stem cells are considered to be the most appropriate. This review is devoted to new methods of diabetes mellitus treatment by using stem cells and perspectives of using C-kit- positive pancreas cells and desmin-positive stellate pancreas cells as the main candidate to the role of pancreatic stem cells.
Halloysite nanotubes is a promising biocompatible material for «smart» composites with encapsulation of biologically active substances
Authors: Rozhina E. V , Danilushkina A. A , Naumenko EA. A , Lvov U. M , Fahrullin R. F
Published: October 2024

Halloysite clay mineral is found in kaolin deposits and is a multilayered nanotube with external diameter of 50 nm inner diameter 15 nm and length1-2 micron. Halloysite is a rolled kaolin and it has found applications in fine porcelain, polymeric composites, tissue engineering, antimicrobial coatings and in cosmetics. Halloysite is also applied as microvesicles to carry drugs and enzymes. It is used as an additive to improve the mechanical strength of polymers. Halloysite is non-toxic to living organisms and the environmentally safe. This review summarizes recent data on the structure of halloysite and its applications.
Cell technologies for muscle tissue restoration. Part II: skeletal and smooth muscles
Authors: Korsakov I. N , Zorin V. L , Eremin I. I , Zorina A. I , Kotenko K. V , Pulin A. A
Published: October 2024

This review represents ongoing clinical studies devoted to recovery of muscle tissue using cell technologies, as well as analysis of applied populations of cells.
Related allogeneic cord blood and bone marrow hematopoietic stem cell transplantation for Fanconi anemia: a case report
Authors: Shul'ga S. Y , Boyarchuk N. G , Potapov I. V , Skorobogatova E. V , Dyshlevaya Z. M , Donyush E. K , Prihodko A. V , Isaev A. A
Published: October 2024

Successful cord blood and bone marrow hematopoietic stem cell transplantation for Fanconi anemia described. The feature of this clinical case is using cord blood unit from private cord blood bank. Private cord blood banks in the future can make a significant contribution to the structure of the autologous and allogeneic transplantations in oncohematology.
The role of pancreatic stellate cells in pancreas regeneration in copper-deficient diet model in rats
Authors: Abdulkhakova A. R , Galyavieva A. R , Abdulkhakov S. R , Trondin AA. A , Pevnev G. O , Titova MA. A , Gumerova A. A , Kiassov A. P
Published: October 2024

The possibility of pancreatic cells transdifferentiation to hepatocyte-like cells in rat copper depletion-repletion model has been shown in a number of articles. The aim of our research was to study the role of pancreatic stellate cells in pancreas regeneration in the above mentioned model. 24 male Wistar rats were fed a copper-deficient diet, containing triethylenetetramine tetrahydrochloride, for a period of 8 weeks, then animals were returned to the normal rat chow for up to a period of 8 weeks. Animals were killed at the end of 4, 6, 8 weeks of copper-deficient diet and 4, 6, 8 weeks after they were returned to normal diet. Paraffin-embedded sections of pancreas were stained immunohistochemically with antibodies to desmin, the pancreatic stellate cells marker. The acinar tissue structure destruction was observed starting from the period of 4 weeks of copper-deficient diet. Partial acinar restoration was observed 6 and 8 weeks after rats were returned to the normal balanced rat chaw. An increase in the number of desmin-positive stellate cells both in pancreatic acinar tissue and in islets was observed at all periods of experiment. Along with the partial acinar tissue recovery desmin-positive cells were found mainly in areas of newly formed pancreatic tissue.
Transformed lung epithelial cells produce microbial RNAse (binase)
Authors: Dudkina E. V , Singh I. , Ulyanova V. V , Shah Mahmud R. , Barreto G. , Ilinskaya O. N
Published: October 2024

Today, the targeted drug delivery to tumors is one of the leading researches in anticancer therapy. Using gene engineering techniques the new expression system based on pCS2+ vector was created. Gene of Bacillus pumilis ribonuclease - binase, and gene of intracellular inhibitor of Bacillus amyloliquefaciens - barstar were cloned under promotor of cytomegalovirus. The possibility of binase's gene expression in transformed murine lung epithelial cells was shown. The new plasmid system provides selective cytotoxic action of binase against target tumor cells due to high expression level of foreign protein in transformed cells.
Isolation, analysis and application of authogenic adipose derived multipotential mesenchymal stromal cells from dog for therapy pseudoarthrosis of tibial bone
Authors: Zakirova E. Y , Zhuravleva M. N , Masgutov R. F , Usmanov RA. A , Rizvanov AA. A
Published: October 2024

This study demonstrates the possibility of using cell-based therapies for the treatment of the longterm intergrows fractures and pseudoarthrosis in dogs. Multipotent mesenchymal stem cells (MMSCs) were isolated from adipose tissue of an adult dog. Multipotent cells was confirmed by the ability to differentiate into 3 main directions (osteogenic, adipogenic and chondrogenic), as well as the expression of the markers CD73, CD105, vimentin. Clinical application received MMSCs in the treatment of pseudoarthrosis of tibia in dogs stimulated bone formation processes. The result of cell therapy was callus formation and recovery of the support function of the injured extremity.
Application of recombinant histone protein H1.3 for inhibition of adenoviral infection
Authors: Solovyeva V. V , Rizvanov A. A
Published: October 2024

There is an ongoing search for new drugs for the treatment of adenovirus infections. In our work the antiviral properties of the recombinant histone H1.3 in in vitro cell culture was investigated and for the first time we demonstrated that recombinant histone H1.3 significantly reduces the efficiency of adenoviral transduction. Also, recombinant histone H1.3 exerts an inhibitory effect on plaque formation on HEK-293A cells monolayer, infected with adenovirus serotype 5, which confirms antiviral properties of histone H1.3 towards adenoviruses.
Rapid Diagnostic Test of Rabies Using Rat Gasser's Ganglion Neurinoma Cell Culture (RGGN-1)
Authors: Khismatullina N. A , Gulyukin A. M , Shuralev E. A , Khaertynov K. S , Chernov A. N , Filimonova M. N , Avzalova A. F , Parshikova A. V , Ivanov A. V
Published: October 2024

The results of the comparative study of the diagnostic efficacy of the method of isolation of street rabies virus using rat Gasser‘s ganglion neurinoma cell culture (RGGN-1) and biological tests on white mice are presented. It is shown that the method of virus isolation using RGGN-1 cell culture is not inferior in the sensitivity to the classic bioassay on white mice and has obvious advantages, such as reducing the time of diagnosis (up to 3 days using RGGN-1 compared to 30 days using white mice) and its cost-effectiveness. Method of cell line RGGN-1infection is recommended for inclusion in the Standards for the rapid diagnosis of rabies and isolation street rabies virus, which should be considered as a possible replacement of bioassays on white mice. Rapid diagnosis of rabies using RGGN-1 cell culture will provide early diagnosis of rabies in animals to reduce the risk of disease in human and animals.
Rapid flow cytofluorometric analysis of functional mitochondrial status in Yarrowia lipolytica yeast: a reasonable model to study human cell bioenergetic responses
Authors: Cherepnev G. V , Igtisamova G. R , Zainullin L. I , Kalacheva N. V , Rizvanov A. A
Published: October 2024

We developed a rapid method to study Yarrowia lipolytica single yeast cell mitochondrial responses based on flow cytometry and potential-sensitive ratiometric fluorochrome JC-1. The proposed model has several advantages as it allows to measure mitochondrial membrane potential in intact cells (without isolation of mitochondria), has an internal positive control (uncoupler of oxidative phosphorylation CCCP), and produces a normalized measurement of the transmembrane mitochondrial potential, taking into account the heterogeneity of cell size and number of mitochondria in a single cell. The similarity of the mitochondrial complex I structure in mammals and Yarrowia lipolytica opens prospects for the model implementation in search for human bioenergetic response modifiers.
Tissue engineering in vascular surgery
Authors: Popov G. I , Vavilov V. N
Published: October 2024

Tissue engineering is a complex biomedical and technological system of knowledge allowing to make and investigate the artificial tissues and organs. Prevalence of vascular diseases and demand of bypass material in vascular surgery led to a lot of researches, with the ultimate aim to create an artificial artery or vein. This article is dedicated to review the main possible methods of artificial vessel manufacturing, some of which already have been used in a clinic.
Effect of cryopreserved nucleated cord blood cells on homeostasis state in animals of different age groups under stress-induced arterial hypertension
Authors: Babiychuk L. V , Babiychuk V. G , Sirotenko LA. A , Malova N. G , Koval S. N
Published: October 2024

Arterial hypertension (AH) might be entirely referred to the socially important pathologies by its prevalence, impact on quality of life and contribution to mortality rate. Nowadays there is accumulated a great number of data testifying to a positive therapeutic effect of cord blood-derived preparations on both different organs and systems, and the whole body as well. This research was aimed to study the dynamics of changes in the indices of heart rate variability (HRV) spectral analysis and lipid homeostasis in blood serum of animals from different age groups under neurogenic stress-induced AH prior to and after administering cryopreserved preparation of cord blood nucleated cells (CB NCs). Neurogenic stress-induced AH caused by emotional and painful stresses was established to be accompanied by a significant decrease in all the studied HRV parameters, as well as disorders in lipid composition of blood serum in rats irrespective of their age. There was observed a decreased activity of autonomic regulation of cardiac rhythm, as well as the tendency to reduction of humoral and metabolic effects. Administration of CB NCs cryopreserved preparation was shown to result in an increased tonus of sympathetic and parasympathetic divisions of autonomic nervous system, as well as humoral link of regulation, that testified to the presence in experimental animals of developed balanced autonomic regulation. In addition the application of CB NCs preparation leveled the state of lipid profile indices, observed at the background of stress-induced AH, and the preparation itself had an antiatherogenic effect and might significantly improve blood rheology.
Current ideas about the a-cells population and their role in the pathogenesis of diabetes mellitus
Authors: Plushkina A. S , Kaligin M. S
Published: October 2024

Diabetes mellitus is a global problem of modern medicine All over the world scientists are study this disease in order to find new and effective therapies Numerous studies in recent years show very important role of glucagon, a hormone produced by α-cells of pancreas, in the pathogenesis of hyperglycemia in diabetes mellitus. The review analyzed current ideas about the features of the formation and differentiation of α- and β-cells of the pancreas. Particular attention is given to α-cells population and their role in the development of hyperglycemia in diabetes mellitus.
The morphological and phenotypic characteristics of microglia at different stages of cultivation and transplantation in the area of spinal cord injury in rats
Authors: Zhuravleva M. N , Mukhamedshina Y. O , Arkhipova S. S , Sanatova E. R , Rizvanov A. A
Published: October 2024

The morphological and phenotypic characteristics of microglia were carried out immediately after isolation from the cerebral cortex of newborn rats, after 24 hours and 2 weeks of cultivation in vitro. It was shown that these cells expressed markers typical for microglia such as Iba1, CD68, CD11b/c, CD45 and Nestin throughout the cultivation period. Microglia had an amoeboid shape initially, but after 2 weeks branched forms of microglia appeared as well Freshly isolated microglia transduced with recombinant lentivirus LV-EGFP was transplanted to the site of spinal cord injury in rats. Transplanted cells survived in acute phase of injury for at least 14 days and expressed reporter EGFP
Plasticity of bone marrow-derived stromal cells at grafting onto neural tissue after ischemic injury in vitro
Authors: Rybachuk O. A , Kyryk V. M , Poberezhnyi P. A , Butenko G. M , Pivneva T. A
Published: October 2024

Bone marrow-derived multipotent mesenchymal stromal cells (BM-MMSCs) are able to confer beneficial effects after transplantation into neural tissue with ischemic injury. This effect is probably caused by the release of trophic factors, although the possibilities of replacement of dead neural cells by BM-MMSCs are not excluded. The aim of this study was to identify the ability of BM-MMScs to differentiate into cells of the nervous tissues and their neuroprotective effect in direct contact with nervous tissue damaged by ischemia Therefore, we investigated this interaction by in vitro model of organotypic hippocampal tissue to avoid affecting the immunological processes in the conditions after transplantation in vivo. Ischemic injury induced by oxygen-glucose deprivation The potential of differentiation of transplanted multipotent mesenchymal bone marrow stromal cells to neural direction was assessed for 14 days after the ischemic injury. At the 7 th day after the oxygen-glucose deprivation and transplantation the multipotent mesenchymal bone marrow stromal cells differentiated into microglial cells, and on the 14th day - as in microglial cells and in mature oligodendrocytes These findings suggest that the transplanted stem cells respond to signals from the microenvironment of the injured tissue of the recipient, which in turn may trigger and regulate cell differentiation as well as to determine the direction of migration
Stimulation of neurogenesis at hippocampus in Alzheimer's disease
Authors: Petukhova E. O , Mukhamedshina Y. O , Vasilieva O. Y , Aksenova L. Y , Solovyeva V. V , Garanina E. E , Rizvanov A. A , Zefirov A. L , Islamov R. R , Mukhamedyarov M. A
Published: October 2024

Alzheimer's disease is a chronic neurodegenerative disease, which is characterized by specific pathomorphological changes in brain including hippocampus One of prospective direction in development of approaches for treatment of Alzheimer's disease is use of gene-cell technologies In present work we studied the possibility to stimulate neurogenesis at hippocampus of APP/PS1 transgenic mice by transplantation of umbilical cord blood mononuclear cells transduced with adenoviral vector overexpressing recombinant glial neurotrophic factor (GDNF). Effectiveness of therapy was evaluated basing on immunoexpression of stem and progenitor cells (using antibodies against doublecortin and nestin) in different hippocampal areas Analysis of obtained data showed that transplantation of umbilical cord blood mononuclear cells overexpressing glial neurotrophic factor or enhanced green fluorescent protein stimulates processes of neurogenesis at hippocampus of transgenic mice with Alzheimer's disease model, certifying high therapeutic potential of these gene-cell constructs
Stimulation of rat's sciatic nerve regeneration by transplantation of human adipose derived multipotent mesenchymal stromal cells
Authors: Masgutov R. F , Masgutova G. A , Salafutdinov I. I , Shulman A. A , Zhuravleva M. N , Gallyamov A. R , Bogov A. A , Bogov A. A , Rizvanov A. A
Published: October 2024

Peripheral nerve injury is a potential threat to the loss of function of an extremity. Despite the ability of neurons to axonal regeneration, nerve injury causes a significant level of neuronal death in spinal ganglia L4-L5, that leads to incomplete functional recovery. The influence of xenotransplantation of human adipose derived multipotent mesenchymal stromal cells on the process of regeneration of rat's sciatic nerve. It is shown that cell therapy contributes to a significant neuronal survival in spinal ganglia L5 after autonerve grafting as well as restore microcirculation and improves recovery of motor activity
Nanocomposite material for stomatology restovration particles cytotoxicity: an in vitro study
Authors: Saleeva G. T , Gimaletdinova A. M , Tarasova E. Y , Rozhina E. V , Naumenko E. A , Fakhrullin R. F , Islamov R. R , Saleev R. A
Published: October 2024

Nanocomposite material applied in dental practice treatment is subjected to mechanical abrasion with the release of material’s fine particles, including nanoparticles, which are able to produce a specific biological effect on patient body’s tissues and cells. I the present research we studied the cytotoxicity of 3MTMESPETMFiltekTMUltimate nanocomposite particles at concentration ranging from 10 to 0. 009 mg/ml employing human lung carcinoma cell culture (A549). Linear dependence of nanocomposite particles’ action on survival and functional activity of the cells was shown. The crucial concentration of cytotoxicity should be considered as 0. 313 mg/ml. At 0.156 mg/ml or below this concentration the tested substance had no toxic effect on A549 cells Considering the possible negative effect of nanocomposite particles released during the material abrasion on cells we suggest limiting application of the studied nanocomposite material on the surfaces subjected to intensive mechanical impact leading to high abrasion
Macrophages: diversity of phenotypes and functions, interaction with foreign materials
Authors: Sarbaeva N. N , Ponomareva J. V , Milyakova M. N
Published: October 2024

According to “M1/M2” paradigm two distinct subsets of macrophages have been proposed - classically (M1) or alternatively (M2) activated macrophages that express different receptors, cytokines, chemokines, growth factors and effector molecules but recent evidence suggests that in respond to changed environmental stimuli they can demonstrate unique properties which do not allow to attribute them neither to M1 nor to M2 population. Macrophages play a pivotal role in foreign body reaction following installation of catheters, stents prosthesis, dental implants Macrophages englobe wear particles around joint prosthesis initiating an inflammation in periprosthetic tissues аnd osteolysis, control fibroproliferation and formation of the fibrous capsule surrounding foreign bodies A brief overview of events leading to migration, adhesion and activation of macrophages, and analysis of their functional characteristics on different surfaces including biodegradable and non-biodegradable materials in vivo and in vitro are presented
Encapsulation of cells and tissues of the pancreas: problems and ways of their overcoming
Authors: Shupletsova V. V , Litvinova L. S , Karpov A. A , Korniushyn O. V , Neimark A. E , Sohonevich N. A , Vasilenko M. A , Dora S. V
Published: October 2024

Despite advances in treatment the patients suffering from diabetes mellitus type 1 have a lifetime shorter the average in population. This is defined primarily by the lack of sufficient glycemic control in these patients The active researches investigating the safety and efficacy of the grafting materials have been carried out in the last decades The review presents modern data on the use of pancreas cells and tissues encapsulation as a possible method for treatment of diabetes type 1 The main problems of the capsules application and possible ways to overcome them were described
INSULIN-PRODUCING CELLS IN THE TREATMENT OF INSULIN-DEPENDENT DIABETES MELLITUS
Authors: Sheremetieva M. Y , Bukharova T. B , Goldstein D. V
Published: October 2024

An effective treatment for insulin-dependent diabetes mellitus (DM), which provides an alternative to hormone replacement therapy, is transplantation of insulin-producing cells (IPCs). Donor β-cells are transplanted both in the form of a complete pancreas, or in the form of isolated islets of Langerhans. However, the application of this method is limited due to the lack of donor material and the need for lifelong immunosuppressive therapy that has a detrimental impact on the weakened DM patient's body. An alternative method of obtaining IPCs is to differentiate stem or progenitor cells. Pancreatic differentiation capability has been demonstrated for various types of stem cells Currently, induced pluripotent stem cell IPSC) are considered to be the most promising source of IPCs, including those obtained from mature cells of the patients themselves Firstly, such IPCs can be gained in unlimited quantities. Secondly, in the case of autologous transplantation they are least exposed to the recipient body's immune attack, thereby making it possible to completely discard immunosuppressive therapy. IPSCs introduction into clinical practice is hindered by the fact that they provoke the formation of teratomas in the recipient>s body. Moreover, they retain this ability even after differentiation because of a number of undifferentiated cells preserved in the population This review focuses on contemporary protocols for obtaining IPCs from IPSCs. These protocols mimic β-cells formation stages during embryonic development. The review also covers the application of IPC immuno-isolating containers for transplantation. Their semipermeable walls, on the one hand, protect the transplant from the recipient>s immune system, and on the other hand, they suppress the risk of the transplant causing tumor formation. in addition, attention will be paid to the use of IPCs derived from IPSCs as a model object for studying the processes occurring in β-cells at normal circumstances as well as during DM.
Application of tissue engineered constructs on the basis of cell sheets FOR RESTORATION OF TISSUES AND ORGANS
Authors: Dergilev K. V , Makarevich P. I , Menshikov M. Yu , Parfyonova E. V
Published: October 2024

Cell sheet technology has certain advantages over conventionally used injections as far as it facilitates cell survival and integration after delivery of cells to intended organ/tissue. It also allows to successfully replace lost or irreversibly damaged tissues with restoration of its functions including endo/paracrine activity. Application of cell sheets has gone beyond bench work and now is under clinical translation where it is successfully used for repair of cornea, cartilage, periodontal tissue, esophageal mucosa, pancreas and thyroid gland. Further advances of cell sheet technologies allow to construct pre-vascularized tissue grafts which effects are not limited to tissue repair, but also allows to restore its function via paracrine action of transplanted cells and to ensure long-lasting therapeutic effects. Genetic modification of cells used for cell sheet construction allows to utilize this technology to treat hereditary disorders, deficit of enzymes or other secreted proteins. This review focuses on recent results of therapeutic implication of cell sheets and prospects of this field which gained much attention in regenerative medicine.
The natural and synthetic polymers of the non-lipid origin in gene delivery
Authors: Bogdanenko E. V , Zhdanov R. I
Published: October 2024

For effective transfection of the eukaryotic cells with the complexes of non-viral gene carriers and plasmids it is necessary to run a number of obstacles so as a gene construction could enter a cellular nucleus and function there successfully and long. Chemical structure of a vector has the crucial importance for the targeted complex delivery to the desired organ. At present polymers of the non-lipid origin are more and more used for gene delivery along with the lipid vectors. In the review advantages and imperfections of some classes of these less used vectors are elucidated depending on their modifications and ratio to DNA and route of the delivery. We concluded that the significant advance in a task of obtaining the effective and safe vector for the human non-viral gene delivery has not been observed yet in spite of designing more and more novel variants of the gene carriers and the new kinds of the plasmids.
Strategies to edit paralogous genes with CRISPR/Cas9
Authors: Nemudryi A. A , Malankhanova T. B , Malakhova A. A , Medvedev S. P , Zakian S. M
Published: October 2024

The purpose of this study is to develop the strategies of CRISPR/Cas9 application to improve fidelity and specificity of this platform. Here we use a model system, which includes target gene and a paralogue - potential aim for off-target double-strand break induction. The study was carried on using Brattleboro rats embryonic fibroblasts which are homozygous for a mutation in arginine-vasopressin gene (target). The potential off-target gene is oxytocin gene: its DNA sequence is almost identical to that of arginine-vasopressin gene. To prevent off-target effect we designed several strategies, which were further used on Brattleboro rats embryonic fibroblasts. Here we show, that these strategies allowed us to generate double-strand breaks in arginine-vasopressin gene without any off-target effects in oxytocin gene. The endonuclease restriction assay shows that we have modified arginine-vasopressin gene while using both CRISPR/Cas9 and single-stranded oligonucleotides as a donor for homologous recombination. At last, if we consider Brattleboro rats as a model of monogenic disease the strategies designed could be translated in human therapeutic genome editing studies.
Effect of modified coatings titanium implants on innate immunity cell
Authors: Plekhova l N. G , Lyapun I. N , Pustovalov E. V , Prosekova E. V , Gnedenkov S. V , Sinebryukhov S. L , Puz A. V
Published: October 2024

The aim of the investigation was to investigate of the functional state effector inflammatory cells (macrophages, neutrophils) in contact with new anti-corrosion osteoinductive coatings deposited on titanium ВТ1-0. The architectonics of cellular surface, morphology, metabolism and production of cytokines by neutrophils and macrophages in their contact with titanium without coating, with the calcium phosphate coated on titanium deposited by plasma electrolytic oxidation (PEO) and PEO-coated with hydroxyapatite were studied. It was established that the most active cells adhered to the surface of the hydroxyapatite-coated titanium, while for titanium without and with calcium-phosphate PEO coated the number of these cells was significantly lower. The study of enzymes showed maximum of stimulation cellular metabolism during the first hour of contact with the coating, further, indicators of enzyme activity decreased in contrast to cells contacted with titanium. The most marked stimulation of the cellular antioxidant protection were detected in contact with a hydroxyapatite-coating. Meanwhile, the calcium-phosphate coating showed lowest immunostimulatory effect, as evidenced by indicators of tissue mediator production: cationic proteins, pro- and antiinflammatory cytokines. The coatings of titanium formed by PEO have a corrective effect on the functional state of innate immune cells reducing inflammation that develops at the foci of implant introducing.
Magnetophoretic properties of human fetal fibroblasts magnetically labeled with citrate stabilized superparamagnetic iron oxide nanoparticles
Authors: Turchyn V. V , Legenkiy Yu. A , Solopov M. V , Popandopulo A. G , Bespalova S. V , E.Ya. Fistal I. Ya
Published: October 2024

Cell transplantology is a perspective and actively developing field of regenerative medicine, but its effectiveness often remains relatively low for some indications. Appliance of magnetic nanoparticles and magnetic fields can increase efficacy of cell transplantation. Superparamagnetic iron oxide nanoparticles (SPION) is a most prospective type of magnetic nanoparticles for magnetically controlled targeting of cells in vivo. In the investigation human fetal fibroblasts were labeled with citrate stabilized SPION (SPION-Cit) that refer to anionic magnetic nanoparticles. 100, 200, 300 and 500 дд Fe/ml doses of magnetic nanoparticles and incubation times of 1, 2 and 3 h were used. The effectiveness of magnetic labeling of cells was evaluated by magnetophoresis in a special chamber using disk NdFeB magnet with diameter of 6 mm and thickness of 3 mm and field induction of 0,255 T: magnetophoretic velocity of magnetized cells in magnetic field gradient was assessed and their magnetic susceptibility was calculated. Viability of magnetically labeled cells was evaluated by trypan blue staining and MTT-test. The value of magnetic susceptibility of magnetically labeled fetal fibroblasts was dose and incubation time depended. A capture distance of labeled cell with the magnet was in a range of 3-4 mm. Magnetophoretic movement of control fibroblasts was not observed. Viability of labeled cells was not decreased substantially in ranges of nanoparticles concentrations 100-300 дд Fe/ml and incubation times 1-3 h. In the concentration of 500 дд Fe/ml partial cell death and exfoliation of cell layer from culture flask observed (signs of low grade exfoliation also observed in the labeling conditions of 300 дд Fe/ml for 3 h). According to our data, most optimal conditions for magnetic labeling of human fetal fibroblast with SPION-Cit is particles concentration 100 дд Fe/ml for 3 h, which provides the capture distance of labeled cells with the magnet about 4 mm.
As figueiras como árvores das encruzilhadas arqueológicas do Passo dos Negros - Pelotas – RS
Authors: Fernandes Mathias Simone , Fernandes Mathias Morales Patrícia , Baptista Carle Cláudio
Published: October 2024

Neste artigo apresenta se reflexões sobre o Passo dos Negros, território indígena e afrocentrado onde se deu o inicío da cidade de Pelotas - RS. Neste espaço á figueiras centenárias que convivem com humanos e não humanos. Buscamos uma perspectiva exunêutica interligada com as encruzilhadas do imaginário arqueológico e antropológico. Compreendendo-as como importantes para a sustentação de uma teoria social insurgente,onde as interpretações e maneiras se consolidam atributos no fazer ciência pelas imagens. Entre o passado e o presente a uma (re) ligação com as águas ancestrais que rodeiam esse espaço, nessa cidade negra e feminina abençoada pelas mães das águas e protegida por Exu (Laroyê!) senhor dos caminhos e dos destinos.
regulação dos corpos
Authors: Mesquita Jesus Francisca
Published: October 2024

O presente trabalho se propõe a reflexão de como se estabelece a lógica de institucionalização no âmbito manicomial, com um recorte racial, por entendermos relevante diante ainda dos poucos trabalhos existentes no Brasil, e que ainda há uma lacuna história da discussão com recorte de raça e cor no âmbito manicomial da institucionalidade interseccionada com corpos e corpas fora de um contexto padronizado e eurocentrado. Os atravessamentos que levam a institucionalização manicomial vão além da doença, fatores estruturais, econômicos e sociais se tornam contribuintes desse adoecimento muitas vezes de ordem sistemática e dentro de um contexto pouco discutido considerando recortes.
Ways to Create a Narrative about the Past in Ukrainian Historical Politics at the Beginning of the 19th Century
Authors: Alexander Gronsky
Published: October 2024

In this article, the author investigated how the historical policy of Ukraine was formed at the beginning of the 19th century, in order to legitimize the Ukrainian historical myth of creating its own statehood. Within the framework of such a process of cultural and historical engineering, ideological patterns are hidden under the guise of academic research, which are broadcast by science, publicism and school education. The author describes the ways of nationalizing history
The communication and action oriented approach in the practice of university teaching
Authors: Vysotskaya I.V.
Published: October 2024

The work is devoted to the theory and practice of the communication and action oriented approach. The purpose of the work is to describe its formation. The scientific works in line with this direction and the experience of its practical implementation at different levels of education in Novosibirsk are the material of the study. The main research methods are the analysis of sources (first of all, the materials of the journal "Diskurs") and the generalization of pedagogical experience at Novosibirsk State Pedagogical University and Novosibirsk State University. The question is raised about the need to implement a communicative and action oriented approach in the practice of university teaching. Some exercises such as "round table", reader's conference, reading with stops / close test, tested in a number of training courses (including the course "Fundamentals of Pedagogical Activity in the Higher Education System" have been proposed, the methodology and conditions for their implementation have been described. The feedback from graduate students of various specialties confirms the possibility of using elements of communicative didactics in relation to a wide range of academic disciplines. In conclusion, it is noted that dialogue not only contributes to the formation of communicative competencies and teamwork skills, but is also away of mastering subject knowledge. The practice of communication is no less important than familiarity with the theory of communication, both in the preparation of professionals in the field of communication technologies, and teachers of all levels of education, as well as other specialists.
Quality of life and subjective well-being of children and caregivers at different stages of allogeneic hematopoietic stem cell transplantation
Authors: Nikolskaya N. S. , Khain A. E. , Evdokimova M. A. , Shelikhova L. N. , Skvortsova Yu. V.
Published: October 2024

This paper presents the results of our study of the subjective assessment of patients and their parents of the impact of disease and treatment with hematopoietic stem cell transplantation (HSCT) on the quality of their daily life and feeling of well-being, as well as of individual and family psychological factors associated with the quality of life and well-being. The data obtained from this study are particularly relevant for the development of long-term, comprehensive programs of psychological followup and rehabilitation of patients during and after HSCT. Despite a constant research interest in changes in the quality of life of patients of different ages and diagnoses undergoing HSCT, there are some discrepancies in the reported results and a lack of longitudinal studies where the parameters of interest are measured before transplantation. Here, were report the initial results of our prospective study that evaluated the quality of life and measures of subjective well-being of patients aged 8–18 years with hematological malignancies, immune and blood diseases and their parents during the preparation for transplantation, and then 3–4 weeks, and 3 months after HSCT. The study was approved by the Independent Ethics Committee and the Scientific Council of the Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology, Ministry of Healthcare of the Russian Federation.
Fibrocartilaginous mesenchymoma: a case report and a literature review
Authors: Karyagina A. A. , Roshchin V. Yu. , Sidorov I. V. , Konovalov D. M.
Published: October 2024

Fibrocartilaginous mesenchymoma of the bone is an extremely rare, locally aggressive tumor which is most often found in the long bones of younger patients. Only about 36 cases have been described in the world literature since 1984. Here, we report a rare clinical case of fibrocartilaginous mesenchymoma of the bone in a 13-year-old child. The patient's parents gave consent to the use of their child's data, including photographs, for research purposes and in publications.
Potential mechanisms of neurogenic tumor maturation
Authors: Zverev I. A. , Druy A. E.
Published: October 2024

In the past years, a significant progress has been achieved in the development of techniques to study morphology and molecular processes within tissues, single cells, and even subcellular structures. This has led to qualitatively new insights into the causes of certain previously unexplained clinical phenomena in oncology, including the rare and paradoxical ability of malignant tumors to become benign. In this review, we critically analyze the existing hypotheses regarding the mechanisms underlying neurogenic tumor maturation, taking into consideration recent data on their origins and biological properties. We also evaluate the potential implications of this knowledge for clinical practice.