bluuebunny/crossref_metadata_2025 huggingface dataset:
Predictive Modeling of Microbial Behavior in Food
Authors: Stavropoulou E. , Bezirtzoglou E.
Published: December 2019

Microorganisms can contaminate food, thus causing food spoilage and health risks when the food is consumed. Foods are not sterile; they have a natural flora and a transient flora reflecting their environment. To ensure food is safe, we must destroy these microorganisms or prevent their growth. Recurring hazards due to lapses in the handling, processing, and distribution of foods cannot be solved by obsolete methods and inadequate proposals. They require positive approach and resolution through the pooling of accumulated knowledge. As the industrial domain evolves rapidly and we are faced with pressures to continually improve both products and processes, a considerable competitive advantage can be gained by the introduction of predictive modeling in the food industry. Research and development capital concerns of the industry have been preserved by investigating the plethora of factors able to react on the final product. The presence of microorganisms in foods is critical for the quality of the food. However, microbial behavior is closely related to the properties of food itself such as water activity, pH, storage conditions, temperature, and relative humidity. The effect of these factors together contributing to permitting growth of microorganisms in foods can be predicted by mathematical modeling issued from quantitative studies on microbial populations. The use of predictive models permits us to evaluate shifts in microbial numbers in foods from harvesting to production, thus having a permanent and objective evaluation of the involving parameters. In this vein, predictive microbiology is the study of the microbial behavior in relation to certain environmental conditions, which assure food quality and safety. Microbial responses are evaluated through developed mathematical models, which must be validated for the specific case. As a result, predictive microbiology modeling is a useful tool to be applied for quantitative risk assessment. Herein, we review the predictive models that have been adapted for improvement of the food industry chain through a built virtual prototype of the final product or a process reflecting real-world conditions. It is then expected that predictive models are, nowadays, a useful and valuable tool in research as well as in industrial food conservation processes.
China’s New Cooperative Medical Scheme’s Impact on the Medical Expenses of Elderly Rural Migrants
Authors: Li Jiajing , Huang Yanran , Nicholas Stephen , Wang Jian
Published: December 2019

Background: With rapid urbanization in China, the scale of elderly migrants from rural areas to urban cities has increased rapidly from 5.03 million in 2000 to 13.4 million people in 2015. Methods: Based on the unbalanced panel data obtained from the Chinese Longitudinal Healthy Longevity Survey, this study investigates the impact of changes to the New Cooperative Medical Scheme (NCMS) on the medical expenditure of Chinese elderly rural migrants by using seemingly unrelated regression models. Results: NCMS coverage for elderly rural migrants rose from 11.83% in 2005 to 87.33% in 2014. The effective reimbursement rate increased significantly from 4.53% in 2005 to 36.44% in 2014, and out-of-pocket/income fell by 50% between 2005 and 2014. The NCMS significantly increased the effective reimbursement rate by 12.4% and out-of-pocket medical expenditure/income by 7.5% during this decade but played an insignificant role in reducing out-of-pocket payments. Conclusions: Policy makers need to promote a two-pronged strategy, which involves controlling the excessive growth of urban medical expenses and continuing to reform NCMS reimbursements for medical treatment, so non-urban resident elderly rural migrants can fully enjoy the welfare benefits of migration and urbanization.
Association between Exposure to Extreme Temperature and Injury at the Workplace
Authors: Lee Junhyeong , Lee Wanhyung , Choi Won-Jun , Kang Seong-Kyu , Ham Seunghon
Published: December 2019

Exposure to extreme temperature is a critical occupational risk factor. This study aimed to investigate the association between exposure to extreme temperatures and injury at the workplace using data from 92,238 workers (46,175 male and 46,063 female) from the 2014 and 2017 Korean Working Condition Survey. Exposure to extremely high or low temperatures, injury experiences, and personal protective equipment (PPE) wearing behavior were investigated using a questionnaire. Logistic regression analyses were performed to investigate the association between exposure to extreme temperature and injury experience. The association between injury experience and PPE wearing behavior was analyzed for each exposure group. After adjusting for individual and occupational factors, the odds ratios (ORs) for injury experience were 2.06 (95% confidence interval (CI): 1.78–2.38) and 1.64 (95% CI: 1.44–1.85) in both high and low temperature exposure groups, respectively, and 1.45 (95% CI: 1.15–1.83) for those not wearing PPE when exposed to high temperature. There was no significant association shown with wearing PPE and injury experience in the low temperature exposure group. Exposure to extreme temperature tended to increase the risk of injury, and was higher in workers not wearing PPE in high temperature. PPE that can be worn comfortably in high temperature is needed to prevent occupational injury.
Physical Attributes of Housing and Elderly Health: A New Dynamic Perspective
Authors: Yang Zan , Fu Yuqi
Published: December 2019

Maintaining health and improving the quality of life of the elderly is extremely challenging in an aging society. In this study, the relationship between housing and the independence and functional capabilities of the elderly is examined, and the effect of housing conditions on health improvements and their economic benefits for the elderly in terms of medical expenditures are assessed. The study is based on the Chinese Health and Retirement Longitudinal Study (CHARLS), which was conducted in 2011 and 2013. Two indices that measure housing conditions and the health status of the elderly were run through regression and state-transition models. Housing was found to have a positive relationship with the health of the elderly, and the improvement of housing conditions could significantly change health status and decrease medical expenditures. The importance of maintaining the health of the elderly through housing adaptations and the economic benefits of housing interventions are highlighted, as these can contribute to both public health and housing adaption subsidy policies.
Friends or Frenemies? The Role of Social Technology in the Lives of Older People
Authors: ten Bruggencate Tina , Luijkx Katrien G. , Sturm Janienke
Published: December 2019

By having a healthy and happy social life, social needs are fulfilled. When social needs are not fulfilled, loneliness and social isolation can occur, which have negative consequences for one’s physical and mental health. Social technology, technology that enables social interaction, can be a resource to fulfil the social needs of older people. In this study, we aimed to learn what role social technology plays in the social life of older people. We held 15 interviews with people aged over 70 who regularly use some form of social technology. Our results indicate that social technology plays different roles in the lives of older people. It strengthens the existing social relationships and social structures. It also brings depth and fun to the social contacts of older people and in this way, enriches their social lives. Social technology also gives a sense of safety and peace of mind to the older people themselves but also to their network members. However, there are barriers in the use of social technology. The older people struggled with using social technology and feel that social technology sometimes stands in the way of real human contact. In supporting and facilitating people’s relationship with others, a community and society, technology helps fulfil older people’s need for connectedness, meaningfulness and independence. However, the relationship with independence is ambiguous. Their life experience gives older people a thoughtful way of looking at social technology and the role it plays in their lives.
Pathogenomics of Uterine Fibroids Development
Authors: Baranov Vladislav S. , Osinovskaya Natalia S. , Yarmolinskaya Maria I.
Published: December 2019

We review recent studies dealing with the molecular genetics and basic results of omics analysis of uterine leiomyoma (LM)—a common benign muscle tumor of the uterus. Whole genome studies of LM resulted in the discovery of many new gene nets and biological pathways, including its origin, transcriptomic, and epigenetic profiles, as well as the impact of the inter-cell matrix in LM growth and involvement of microRNA in its regulation. New data on somatic cell mutations ultimately involved in the origin, distribution and growth of LM are reviewed. Putative identification of LM progenitor SC (stem cells) giving rise to maternal fibroid nodes and junctional zones provide a new clue for hypotheses on the pathogenomics of LM. The reviewed data are consistent with at least two different but probably intimately interacted molecular mechanisms of LM. One of them (the genetic hypothesis) is focused primarily on the MED12 gene mutations and suggests its onset in the side population of embryonic myoblasts of the female reproductive system, which later gave rise to multiple small and medium fibroids. The single and usually large-size fibroids are induced by predominantly epigenetic disorders in LM SC, provoked by enhanced expression of the HMGA2 gene caused by its hypomethylation and epigenetic deregulation enhanced by hypoxia, muscle tension, or chromosome instability/aberrations. The pathogenomics of both genetic and epigenetic programs of LM with many peculiarities at the beginning later became rather similar and partly overlapped due to the proximity of their gene nets and epigenetic landscape. Pathogenomic studies of LM open ways for elaboration of novel strategies of prevention and treatment of this common disease.
Toll-Like Receptor Mediated Activation of Natural Autoantibody Producing B Cell Subpopulations in an Autoimmune Disease Model
Authors: Erdő-Bonyár Szabina , Rapp Judit , Minier Tünde , Ráth Gábor , Najbauer József , Czirják László , Németh Péter , Berki Timea , Simon Diána
Published: December 2019

Altered expression and function of the Toll-like receptor (TLR) homologue CD180 molecule in B cells have been associated with autoimmune disorders. In this study, we report decreased expression of CD180 at protein and mRNA levels in peripheral blood B cells of diffuse cutaneous systemic sclerosis (dcSSc) patients. To analyze the effect of CD180 stimulation, together with CpG (TLR9 ligand) treatment, on the phenotype defined by CD19/CD27/IgD/CD24/CD38 staining, and function (CD69 and CD180 expression, cytokine and antibody secretion) of B cell subpopulations, we used tonsillar B cells. After stimulation, we found reduced expression of CD180 protein and mRNA in total B cells, and CD180 protein in B cell subpopulations. The frequency of CD180+ cells was the highest in the CD19+CD27+IgD+ non-switched (NS) B cell subset, and they showed the strongest activation after anti-CD180 stimulation. Furthermore, B cell activation via CD180 induced IL-6 and natural autoantibody secretion. Treatment with the combination of anti-CD180 antibody and CpG resulted in increased IL-6 and IL-10 secretion and natural autoantibody production of B cells. Our results support the role of CD180 in the induction of natural autoantibody production, possibly by NS B cells, and suggest an imbalance between the pathologic and natural autoantibody production in SSc patients.
Optical and Thermal Properties of Laser-Ablated Platinum Nanoparticles Graphene Oxide Composite
Authors: Sadrolhosseini Amir Reza , Habibiasr Mina , Shafie Suhaidi , Solaimani Hassan , Lim Hong Ngee
Published: December 2019

Platinum nanoparticles were synthesized in graphene oxide aqueous solution using a laser ablation technique to investigate the effect of optical linear, nonlinear and thermal properties of platinum-graphene oxide nanocomposite solution. The samples were prepared with different ablation times. The platinum nanoparticles that formed a spherical shape on the surface of graphene oxide solution were authenticated using UV-visible spectrum and transmission electron microscopy patterns. The particle size decreased with increasing ablation time, and the concentration and volume fraction of samples were increased. To obtain the optical linear, nonlinear and thermal properties of platinum-graphene oxide nanocomposite solution, UV-visible spectroscopy, Z-scan, thermal lens and photoacoustic techniques were used. Consequently, the linear and nonlinear refractive indices increased with an increase in the volume fraction of platinum nanoparticles. It was observed from the spatial self-phase modulation patterns that, the optical nonlinear property of the graphene oxide was enhanced in the presence of platinum nanoparticles, and the nonlinearity increased with an increase in the volume fraction of platinum nanoparticles inside the graphene oxide solution. The thermal diffusivity and thermal effusivity of platinum nanoparticles graphene oxide were measured using a thermal lens and photoacoustic methods, respectively. The thermal diffusivity and thermal effusivity of samples were in the range of 0.0341 × 10−5 m2/s to 0.1223 × 10−5 m2/s and 0.163 W s1/2 cm−2 K−1 to 0.3192 W s1/2 cm−2 K−1, respectively. Consequently, the platinum enhanced the optical and thermal properties of graphene oxide.
The Integrin Activating Protein Kindlin-3 Is Cleaved in Human Platelets during ST-Elevation Myocardial Infarction
Authors: Kraemer Bjoern F. , Lamkemeyer Tobias , Franz-Wachtel Mirita , Lindemann Stephan
Published: December 2019

Kindlins are important proteins for integrin signaling and regulation of the cytoskeleton, but we know little about their precise function and regulation in platelets during acute ischemic events. In this work, we investigated kindlin-3 protein levels in platelets isolated from patients with ST-elevation myocardial infarction (STEMI) compared to patients with non-ischemic chest pain. Platelets from twelve patients with STEMI and twelve patients with non-ischemic chest pain were isolated and analyzed for kindlin-3 protein levels and intracellular localization by immunoblotting and two-dimensional gel electrophoresis. Platelet proteome analysis by two-dimensional gel electrophoresis and protein sequencing identified kindlin-3 as a protein that is cleaved in platelets from patients with myocardial infarction. Kindlin-3 full-length protein was significantly decreased in patients with STEMI compared to patients with non-ischemic chest pain (1.0 ± 0.2 versus 0.28 ± 0.2, p < 0.05) by immunoblotting. Kindlin-3 showed a differential distribution and was primarily cleaved in the cytosolic and membrane compartment of platelets in myocardial infarction. Platelet activation with thrombin alone did not affect kindlin-3 protein levels. The present study demonstrates that kindlin-3 protein levels become significantly reduced in platelets of patients with myocardial infarction compared to controls. The results suggest that kindlin-3 cleavage in platelets is associated with the ischemic event of myocardial infarction.
The Impact of p53 on Aristolochic Acid I-Induced Gene Expression In Vivo
Authors: Sborchia Mateja , Keun Hector C. , Phillips David H. , Arlt Volker M.
Published: December 2019

Exposure to aristolochic acid (AA) is linked to kidney disease and urothelial cancer in humans. The major carcinogenic component of the AA plant extract is aristolochic acid I (AAI). The tumour suppressor p53 is frequently mutated in AA-induced tumours. We previously showed that p53 protects from AAI-induced renal proximal tubular injury, but the underlying mechanism(s) involved remain to be further explored. In the present study, we investigated the impact of p53 on AAI-induced gene expression by treating Trp53(+/+), Trp53(+/-), and Trp53(-/-) mice with 3.5 mg/kg body weight (bw) AAI daily for six days. The Clariom™ S Assay microarray was used to elucidate gene expression profiles in mouse kidneys after AAI treatment. Analyses in Qlucore Omics Explorer showed that gene expression in AAI-exposed kidneys is treatment-dependent. However, gene expression profiles did not segregate in a clear-cut manner according to Trp53 genotype, hence further investigations were performed by pathway analysis with MetaCore™. Several pathways were significantly altered to varying degrees for AAI-exposed kidneys. Apoptotic pathways were modulated in Trp53(+/+) kidneys; whereas oncogenic and pro-survival pathways were significantly altered for Trp53(+/-) and Trp53(-/-) kidneys, respectively. Alterations of biological processes by AAI in mouse kidneys could explain the mechanisms by which p53 protects from or p53 loss drives AAI-induced renal injury in vivo.
Osteoarthritis and Cartilage Regeneration: Focus on Pathophysiology and Molecular Mechanisms
Authors: Grässel Susanne , Aszodi Attila
Published: December 2019

Osteoarthritis (OA) is a leading cause of disability and source of societal cost in older adults [...]
Phosphorylation Dynamics of JNK Signaling: Effects of Dual-Specificity Phosphatases (DUSPs) on the JNK Pathway
Authors: Ha Jain , Kang Eunjeong , Seo Jihye , Cho Sayeon
Published: December 2019

Protein phosphorylation affects conformational change, interaction, catalytic activity, and subcellular localization of proteins. Because the post-modification of proteins regulates diverse cellular signaling pathways, the precise control of phosphorylation states is essential for maintaining cellular homeostasis. Kinases function as phosphorylating enzymes, and phosphatases dephosphorylate their target substrates, typically in a much shorter time. The c-Jun N-terminal kinase (JNK) signaling pathway, a mitogen-activated protein kinase pathway, is regulated by a cascade of kinases and in turn regulates other physiological processes, such as cell differentiation, apoptosis, neuronal functions, and embryonic development. However, the activation of the JNK pathway is also implicated in human pathologies such as cancer, neurodegenerative diseases, and inflammatory diseases. Therefore, the proper balance between activation and inactivation of the JNK pathway needs to be tightly regulated. Dual specificity phosphatases (DUSPs) regulate the magnitude and duration of signal transduction of the JNK pathway by dephosphorylating their substrates. In this review, we will discuss the dynamics of phosphorylation/dephosphorylation, the mechanism of JNK pathway regulation by DUSPs, and the new possibilities of targeting DUSPs in JNK-related diseases elucidated in recent studies.
Metabolic Health—The Role of Adipo-Myokines
Authors: Graf Christine , Ferrari Nina
Published: December 2019

Obesity is now a worldwide epidemic. In recent years, different phenotypes of obesity, ranging from metabolically healthy normal weight to metabolically unhealthy obese, were described. Although there is no standardized definition for these phenotypes or for metabolic health, the influence of lifestyle and early-life factors is undisputed. In this context, the ratio of muscle-to-fat tissue seems to play a crucial role. Both adipose tissue and skeletal muscle are highly heterogeneous endocrine organs secreting several hormones, with myokines and adipokines being involved in local autocrine/paracrine interactions and crosstalk with other tissues. Some of these endocrine factors are secreted by both tissues and are, therefore, termed adipo-myokines. High (cardiorespiratory) fitness as a surrogate parameter for an active lifestyle is epidemiologically linked to “better” metabolic health, even in the obese; this may be partly due to the role of adipo-myokines and the crosstalk between adipose and muscle tissue. Therefore, it is essential to consider (cardiovascular) fitness in the definition of metabolically healthy obese/metabolic health and to perform longitudinal studies in this regard. A better understanding of both the (early-life) lifestyle factors and the underlying mechanisms that mediate different phenotypes is necessary for the tailored prevention and personalized treatment of obesity.
Quantitative Proteomic Analysis Uncovers the Mediation of Endoplasmic Reticulum Stress-Induced Autophagy in DHAV-1-Infected DEF Cells
Authors: Lan Jingjing , Zhang Ruihua , Yu Honglei , Wang Jingyu , Xue Wenxiang , Chen Junhao , Lin Shaoli , Wang Yu , Xie Zhijing , Jiang Shijin
Published: December 2019

Autophagy is a tightly regulated catabolic process and is activated in cells in response to stress signals. Despite extensive study, the interplay between duck hepatitis A virus type 1 (DHAV-1) and the autophagy of host cells is not clear. In this study, we applied proteomics analysis to investigate the interaction mechanism between DHAV-1 and duck embryo fibroblast (DEF) cells. In total, 507 differentially expressed proteins (DEPs) were identified, with 171 upregulated proteins and 336 downregulated proteins. The protein expression level of heat shock proteins (Hsps) and their response to stimulus proteins and zinc finger proteins (ZFPs) were significantly increased while the same aspects of ribosome proteins declined. Bioinformatics analysis indicated that DEPs were mainly involved in the “response to stimulus”, the “defense response to virus”, and the “phagosome pathway”. Furthermore, Western blot results showed that the conversion of microtubule-associated protein 1 light chain 3-I (LC3-I) to the lipidation form of LC3-II increased, and the conversion rate decreased when DEF cells were processed with 4-phenylbutyrate (4-PBA). These findings indicated that DHAV-1 infection could cause endoplasmic reticulum (ER) stress-induced autophagy in DEF cells, and that ER stress was an important regulatory factor in the activation of autophagy. Our data provide a new clue regarding the host cell response to DHAV-1 and identify proteins involved in the DHAV-1 infection process or the ER stress-induced autophagy process.
Development of a Highly Efficient Hybrid Peptide That Increases Immunomodulatory Activity Via the TLR4-Mediated Nuclear Factor-κB Signaling Pathway
Authors: Zhang Lulu , Wei Xubiao , Zhang Rijun , Koci Matthew , Si Dayong , Ahmad Baseer , Cheng Junhao , Wang Junyong
Published: December 2019

Immunity is a defensive response that fights disease by identifying and destroying harmful substances or microbiological toxins. Several factors, including work-related stress, pollution, and immunosuppressive agents, contribute to low immunity and poor health. Native peptides, a new class of immunoregulatory agents, have the potential for treating immunodeficiencies, malignancies, and infections. However, the potential cytotoxicity and low immunoregulatory activity and stability of native peptides have prevented their development. Therefore, we designed three hybrid peptides (LTAa, LTAb, and LTAc) by combining a characteristic fragment of LL-37 with an active Tα1 center that included Tα1 (17–24), Tα1 (20–25), and Tα1 (20–27). The best hybrid peptide (LTAa), according to molecule docking and in vitro experiments, had improved immunoregulatory activity and stability with minimal cytotoxicity. We investigated the immunoregulatory effects and mechanisms of LTAa using a cyclophosphamide-immunosuppressed murine model. LTAa effectively reversed immunosuppression by enhancing immune organ development, activating peritoneal macrophage phagocytosis, regulating T lymphocyte subsets, and increasing cytokine (tumor necrosis factor-alpha, interleukin-6, and interleukin-1β) and immunoglobulin (IgA, IgG, and IgM) contents. The immunomodulatory effects of LTAa may be associated with binding to the TLR4/MD-2 complex and activation of the NF-κB signaling pathway. Therefore, LTAa could be an effective therapeutic agent for improving immune function.
New Insight Regarding the Relationship Between Enantioselective Toxicity Difference and Enantiomeric Toxicity Interaction from Chiral Ionic Liquids
Authors: Ge Huilin , Zhou Min , Lv Daizhu , Wang Mingyue , Dong Cunzhu , Wan Yao , Zhang Zhenshan , Wang Suru
Published: December 2019

Chirality is an important property of molecules. The study of biological activity and toxicity of chiral molecules has important theoretical and practical significance for toxicology, pharmacology, and environmental science. The toxicological significance of chiral ionic liquids (ILs) has not been well revealed. In the present study, the enantiomeric joint toxicities of four pairs of chiral ILs 1-alkyl-3-methylimidazolium lactate to Allivibrio fischeri were systematically investigated by using a comprehensive approach including the co-toxicity coefficient (CTC) integrated with confidence interval (CI) method (CTCICI), concentration-response curve (CRC), and isobole analysis. The direct equipartition ray (EquRay) design was used to design five binary mixtures of enantiomers according to molar ratios of 1:5, 2:4, 3:3, 4:2, and 5:1. The toxicities of chiral ILs and their mixtures were determined using the microplate toxicity analysis (MTA) method. Concentration addition (CA) and independent action (IA) were used as the additive reference models to construct the predicted CRC and isobole of mixtures. On the whole, there was an enantioselective toxicity difference between [BMIM]D-Lac and [BMIM]L-Lac, and [HMIM]D-Lac and [HMIM]L-Lac, while no enantioselective toxicity difference was observed for [EMIM]D-Lac and [EMIM]L-Lac, and [OMIM]D-Lac and [OMIM]L-Lac. Thereinto, the enantiomer mixtures of [BMIM]D-Lac and [BMIM]L-Lac, and [HMIM]D-Lac and [HMIM]L-Lac presented antagonistic action, and the enantiomer mixtures of [EMIM]D-Lac and [EMIM]L-Lac, and [OMIM]D-Lac and [OMIM]L-Lac overall presented additive action. Moreover, the greatest antagonistic toxicity interaction occurred at the equimolar ratio of enantiomers. Based on these results, we proposed two hypotheses, (1) chiral molecules with enantioselective toxicity difference tended to produce toxicity interactions, (2) the highest or lowest toxicity was usually at the equimolar ratio and its adjacent ratio for the enantiomer mixture. These hypotheses will need to be further validated by other enantiomer mixtures.
Rice Protein Exerts Anti-Inflammatory Effect in Growing and Adult Rats via Suppressing NF-κB Pathway
Authors: Wang Zhengxuan , Liang Mingcai , Li Hui , Cai Liang , Yang Lin
Published: December 2019

To elucidate the effect of rice protein (RP) on the depression of inflammation, growing and adult rats were fed with caseins and RP for 2 weeks. Compared with casein, RP reduced hepatic accumulations of reactive oxygen species (ROS) and nitro oxide (NO), and plasma activities of alanine transaminase (ALT) and aspartate transaminase (AST) in growing and adult rats. Intake of RP led to increased mRNA levels, and protein expressions of phosphoinositide 3 kinase (PI3K), protein kinase B (Akt), nuclear factor-κB 1 (NF-αB1), reticuloendotheliosis viral oncogene homolog A (RelA), tumor necrotic factor α (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and monocyte chemoattractant protein-1 (MCP-1) were decreased, whereas hepatic expressions of interleukin-10 (IL-10) and heme oxygenase 1 (HO-1) were increased by RP. The activation of NF-κB was suppressed by RP through upregulation of inhibitory κB α (IκBα), resulting in decreased translocation of nuclear factor-κB 1 (p50) and RelA (p65) to the nucleus in RP groups. The present study demonstrates that RP exerts an anti-inflammatory effect to inhibit ROS-derived inflammation through suppression of the NF-κB pathway in growing and adult rats. Results suggest that the anti-inflammatory capacity of RP is independent of age.
A Regulatory Network for miR156-SPL Module in Arabidopsis thaliana
Authors: Zheng Chenfei , Ye Meixia , Sang Mengmeng , Wu Rongling
Published: December 2019

Vegetative phase changes in plants describes the transition between juvenile and adult phases of vegetative growth before flowering. It is one of the most fundamental mechanisms for plants to sense developmental signals, presenting a complex process involving many still-unknown determinants. Several studies in annual and perennial plants have identified the conservative roles of miR156 and its targets, SBP/SPL genes, in guiding the switch of plant growth from juvenile to adult phases. Here, we review recent progress in understanding the regulation of miR156 expression and how miR156-SPLs mediated plant age affect other processes in Arabidopsis. Powerful high-throughput sequencing techniques have provided rich data to systematically study the regulatory mechanisms of miR156 regulation network. From this data, we draw an expanded miR156-regulated network that links plant developmental transition and other fundamental biological processes, gaining novel and broad insight into the molecular mechanisms of plant-age-related processes in Arabidopsis.
The Prognostic Significance of Eukaryotic Translation Initiation Factors (eIFs) in Endometrial Cancer
Authors: Smolle Maria Anna , Czapiewski Piotr , Lapińska-Szumczyk Sylwia , Majewska Hanna , Supernat Anna , Zaczek Anna , Biernat Wojciech , Golob-Schwarzl Nicole , Haybaeck Johannes
Published: December 2019

Whilst the role of eukaryotic translation initiation factors (eIFs) has already been investigated in several human cancers, their role in endometrial cancer (EC) is relatively unknown. In the present retrospective study, 279 patients with EC (1180 samples) were included (mean age: 63.0 years, mean follow-up: 6.1 years). Samples were analysed for expression of 7 eIFs subunits (eIF2α, eIF3c, eIF3h, eIF4e, eIF4g, eIF5, eIF6) through immunohistochemistry and western blotting. Fifteen samples of healthy endometrium served as controls. Density and intensity were assessed and mean combined scores (CS) calculated for each patient. Upon immunohistochemistry, median eIF5 CS were significantly higher in EC as compared with non-neoplastic tissue (NNT, p < 0.001), whilst median eIF6 CS were significantly lower in EC (p < 0.001). Moreover, eIF5 (p = 0.002), eIF6 (p = 0.032) and eIF4g CS (p = 0.014) were significantly different when comparing NNT with EC grading types. Median eIF4g CS was higher in type II EC (p = 0.034). Upon western blot analysis, eIF4g (p < 0.001), peIF2α (p < 0.001) and eIF3h (p < 0.05) were significantly overexpressed in EC, while expression of eIF3c was significantly reduced in EC as compared with NNT (p < 0.001). The remaining eIFs were non-significant. Besides tumour stage (p < 0.001) and patient’s age (p < 0.001), high eIF4g CS-levels were independently associated with poor prognosis (HR: 1.604, 95%CI: 1.037–2.483, p = 0.034). The other eIFs had no prognostic significance. Notably, the independent prognostic significance of eIF4g was lost when adding tumour type. Considering the difficulties in differentiating EC type I and II, eIF4g may serve as a novel prognostic marker indicating patient outcome.
Molecular Modelling of Islet β-Cell Adaptation to Inflammation in Pregnancy and Gestational Diabetes Mellitus
Authors: Lorenzo Petra I. , Martín-Montalvo Alejandro , Cobo Vuilleumier Nadia , Gauthier Benoit R.
Published: December 2019

Gestational diabetes mellitus (GDM), a metabolic disease that develops with the increase in insulin resistance during late pregnancy, is currently one of the most common complications affecting pregnancy. The polygenic nature of GDM, together with the interplay between different genetic variants with nutritional and environmental factors has hindered the full understanding of the etiology of this disease. However, an important genetic overlap has been found with type 2 diabetes mellitus (T2DM) and, as in the case of T2DM, most of the identified loci are associated with β-cell function. Early detection of GDM and adequate interventions to control the maternal glycemia are necessary to avoid the adverse outcomes for both the mother and the offspring. The in utero exposure to the diabetic milieu predispose these children for future diseases, among them T2DM, originating a vicious circle implicated in the increased prevalence of both GDM and T2DM. The involvement of inflammatory processes in the development of GDM highlights the importance of pancreatic β-cell factors able to favor the adaptation processes required during gestation, concomitantly with the protection of the islets from an inflammatory milieu. In this regard, two members of the Pax family of transcription factors, PAX4 and PAX8, together with the chromatin remodeler factor HMG20A, have gained great relevance due to their involvement in β-cell mass adaptation together with their anti-inflammatory properties. Mutations in these factors have been associated with GDM, highlighting these as novel candidates for genetic screening analysis in the identification of women at risk of developing GDM.
Depression, GABA, and Age Correlate with Plasma Levels of Inflammatory Markers
Authors: Bhandage Amol K. , Cunningham Janet L. , Jin Zhe , Shen Qiujin , Bongiovanni Santiago , Korol Sergiy V. , Syk Mikaela , Kamali-Moghaddam Masood , Ekselius Lisa , Birnir Bryndis
Published: December 2019

Immunomodulation is increasingly being recognised as a part of mental diseases. Here, we examined whether levels of immunological protein markers changed with depression, age, or the inhibitory neurotransmitter gamma-aminobutyric acid (GABA). An analysis of plasma samples from patients with a major depressive episode and control blood donors (CBD) revealed the expression of 67 inflammatory markers. Thirteen of these markers displayed augmented levels in patients compared to CBD. Twenty-one markers correlated with the age of the patients, whereas 10 markers correlated with the age of CBD. Interestingly, CST5 and CDCP1 showed the strongest correlation with age in the patients and CBD, respectively. IL-18 was the only marker that correlated with the MADRS-S scores of the patients. Neuronal growth factors (NGFs) were significantly enhanced in plasma from the patients, as was the average plasma GABA concentration. GABA modulated the release of seven cytokines in anti-CD3-stimulated peripheral blood mononuclear cells (PBMCs) from the patients. The study reveals significant changes in the plasma composition of small molecules during depression and identifies potential peripheral biomarkers of the disease.
Expression, Subcellular Localization, and Interactions of CPK Family Genes in Maize
Authors: Khalid Muhammad Hayder Bin , Raza Muhammad Ali , Yu Hao Qiang , Khan Imran , Sun Fu Ai , Feng Ling Yang , Qu Jing Tao , Fu Feng Ling , Li Wan Chen
Published: December 2019

Calcium-dependent protein kinase (CPKs) is a key player in the calcium signaling pathway to decode calcium signals into various physiological responses. cDNA sequences of 9 ZmCPK genes were successfully cloned from all four phylogenetic groups in maize. qRT-PCR analysis showed the expression variation of these selected genes under abscisic acid (ABA) and calcium chloride (CaCl2) treatment. Due to the presence of N-myristoylation/palmitoylation sites, the selected ZmCPK members were localized in a plasma membrane. To clarify whether ZmCPK, a key player in calcium signaling, interacts with key players of ABA, protein phosphatase 2Cs (PP2Cs) and the SNF1-related protein kinase 2s (SnRK2s) and mitogen-activated protein kinase (MAPK) signaling pathways in maize, we examined the interaction between 9 CPKs, 8 PP2Cs, 5 SnRKs, and 20 members of the MPK family in maize by using yeast two-hybrid assay. Our results showed that three ZmCPKs interact with three different members of ZmSnRKs while four ZmCPK members had a positive interaction with 13 members of ZmMPKs in different combinations. These four ZmCPK proteins are from three different groups in maize. These findings of physical interactions between ZmCPKs, ZmSnRKs, and ZmMPKs suggested that these signaling pathways do not only have indirect influence but also have direct crosstalk that may involve the defense mechanism in maize. The present study may improve the understanding of signal transduction in plants.
The Reproduction Ability of a Numerical Model for Simulating the Outflow Rate of Backfilling Materials from a Coastal Structure
Authors: Silarom Kornvisith , Yamamoto Yoshimichi
Published: December 2019

In very shallow areas, the frequency by which coastal structures (like dikes and seawalls) are directly broken by large wave forces is low because large waves are broken in deeper areas. The main cause for such destruction is ground scour in front of the structures and outflow of backfilling materials by middle-scale waves; therefore, the scour and the outflow should be considered when designing a coastal structure in a very shallow area. In this paper, a numerical model consisting of CADMAS-SURF, which can calculate fluid motion in porous media, and empirical equations for simulating the outflow phenomena are introduced; thereafter, practical calculations on field cases in Thailand and Japan are demonstrated. Additionally, since the effects of wave periods and water depth to the outflow rate have never been clarified, hydraulic model experiments, empirical calculations using an existing formula, and numerical simulations are performed in order to examine these effects on the outflow rate. The simulated results using the numerical model align well with the experimental results. Moreover, both results show that the outflow rate is proportional to the wave period and inversely proportional to water depth.
Temporal Variation in Preferential Water Flow during Natural Vegetation Restoration on Abandoned Farmland in the Loess Plateau of China
Authors: Wang Rui , Dong Zhibao , Zhou Zhengchao , Wang Peipei
Published: December 2019

The restoration of vegetation in abandoned farmlands is an effective approach to control soil erosion on the Chinese Loess Plateau. However, few studies have investigated the effect of natural restoration age on the infiltration patterns and preferential flow in soil layers. This study examined the effect of the temporal variations in the degree of preferential flow and their contribution on the total infiltration in abandoned farmlands restored with different vegetation communities. In total, four sites were investigated, namely site AF3, AF12, AF18 and AF25 of restoration age of 3, 12, 18 and 25 years, respectively. A traditional dye tracer experiment was adopted to visualize the water flow behavior in the four sites. A semi-variance analysis was conducted to interpret the spatial variability of the soil water induced by infiltration. The results showed that both the soil properties and the root systems improved with the increased natural restoration ages combined with the effect of the plant species. The degree of preferential flow from the lowest to the highest occurred in AF3, AF12, AF18 and AF25, which was mainly affected by the development of root systems. Also, the abundant lateral root and steeper slope may have caused the presence of lateral flow in AF18 and AF25. Meanwhile, the spatial variability of the soil water in the infiltration patterns through vertical soil profiles increased from AF3 to AF25. Compared to AF3, the infiltration volume through preferential pathways in AF12, AF18 and AF25 increased by 1.73 times, 1.94 times and 4.09 times, respectively. Therefore, the preferential flow was improved in abandoned farmland in the presence of natural vegetation restoration that can increase water storage in the deep soil layer and reduce surface runoff and erosion.
Characteristics of Periodic Ultrasonic Assisted TIG Welding for 2219 Aluminum Alloys
Authors: Cai Xiaoyu , Lin Sanbao , Wang Xianneng , Yang Chunli , Fan Chenglei
Published: December 2019

Tungsten inert gas (TIG) arc welding of 2219 aluminum alloy was assisted with a trailing periodic ultrasonic vibration, which was output from a trailing roller behind the welding torch. It was found that the weld appearance was periodically convex due to the periodic input of ultrasonic vibration. With the addition of ultrasonic vibration, the columnar grains in the weld zone transformed to equiaxed grains, so along the longitudinal direction, the equiaxed grains and the columnar grains were alternately distributed due to the periodic ultrasonic vibration. The effects of different ultrasonic powers were investigated. The penetration depth and the amount of the melting metal both increased as the ultrasonic power increased. The coarse precipitated phases in the weld zone tended to disperse uniformly under ultrasonic vibration. Compared with conventional TIG welded joints, the hardness of the weld zone of the ultrasonic assisted TIG welding increased by 8.43%, and the tensile strength increased by 29.02%. The ultrasonic cavitation could decrease the nucleation radius and break the dendrites, which led to the grains’ refinement and the final mechanical properties’ improvement.
Three-Dimensional Digital Reconstruction of Ti2AlC Ceramic Foams Produced by the Gelcast Method
Authors: Stiapis Christos S. , Skouras Eugene D. , Burganos Vasilis N.
Published: December 2019

A digital reconstruction technique is presented that generates three-dimensional (3D) digital representations of ceramic foams created by the foam-gelcasting technique. The reconstruction process uses information that is directly extracted from Scanning Electron Microscopy (SEM) images and offers a 3D representation of the physical sample accounting for the typically large pore cavities and interconnecting windows that are formed during the preparation process. Contrary to typical tessellation-based foam treatments, a spherical representation of the pores and the pore windows of the foams is assumed and a novel hybrid algorithm that combines a variation of Lubachevsky-type and Random Close Packing of Hard Spheres (RCPHS) algorithms has been developed to obtain near-optimum solutions to the packing problem of the spheres that represent the pores. Numerical simulations are performed directly on the 3D reconstructed foams to determine their gas permeability. The model predictions are compared with experimental gas permeability data that were obtained for the physical samples. The pore wall thickness can be treated as the single fitting parameter in the entire reconstruction process, although it is shown that images of sufficient resolution could eliminate the need even for that. The foams that are produced by this method yield quantitatively similar pressure drops with experiments for various superficial velocity values, with a very small deviation in the range of 1.7–2.8%. The proposed methodology could be utilized for the prediction of the permeability and transport properties of complex foamy porous structures, similar to the gelcast-type of foams, from a single SEM image of the foam sample without resorting to serial tomography or other structural information, thus saving considerable time and effort from experimental work.
Electromagnetic Analysis and Design Methodology for Permanent Magnet Motors Using MotorAnalysis-PM Software
Authors: Kuptsov Vladimir , Fajri Poria , Trzynadlowski Andrzej , Zhang Guoliang , Magdaleno-Adame Salvador
Published: December 2019

This article presents a new and powerful freeware software called MotorAnalysis-PM and discusses its application in electromagnetic design and analysis of permanent magnet (PM) motors for the electric vehicle (EV) industry. This new PM motor software utilizes both finite element (FE) and analytical methods to speed up the analysis and design process of PM motors significantly. The analysis and design methodology using MotorAnalysis-PM is presented and discussed for a 50 kW PM motor utilized in a commercial EV. To validate the accuracy of the software, the numerical results obtained from the PM motor design and analysis tool are compared with experimental results. The numerical and experimental results validate the flexibility of this software in achieving accurate motor design with short design times which is of great interest to EV and PM motor manufacturers.
Dynamic Torque Limitation Principle in the Main Line of a Mill Stand: Explanation and Rationale for Use
Authors: Gasiyarov V.R. , Khramshin V.R. , Voronin S.S. , Lisovskaya T.A. , Gasiyarova O.A.
Published: December 2019

This paper substantiates why it is necessary to limit the dynamic loads in stand shafts when closing angular gaps. The paper is a feasibility study of a concept developed specifically to limit each of the factors that affect amplitude of the dynamic torque; limitations applied are determined on a case-by-case basis. The research team studied the components of elastic torque in a dual-mass electromechanical system of Mill 5000 caused by vibrations arising from the elasticity of transmission as well as from the vibrations caused by elastic collision itself. It is shown that the lowest possible pre-acceleration is necessary for decreasing the dynamic coefficient at a near-nominal load torque. A principle of limiting dynamic loads when closing an angular gap is proposed, which implies collecting tachograms of an electric drive subjected to positive or negative acceleration before and after the fed metal is captured. The research team has developed drive control principles to implement this approach. The recommendation is to upgrade the electric drives of the reversing stand in Mill 5000.
The Pharmacokinetics of Fucoidan after Topical Application to Rats
Authors: Pozharitskaya Olga N. , Shikov Alexander N. , Obluchinskaya Ekaterina D. , Vuorela Heikki
Published: December 2019

Fucoidan, a fucose-rich polysaccharide from brown algae, has been used for transdermal formulations targeting inflammatory skin conditions, for the treatment of thrombosis, vascular permeability diseases, subcutaneous wounds, and burns. However, the pharmacokinetics of fucoidan after topical application has not been described. In this study, an ointment (OF) containing 15% fucoidan was topically applied to rats at the doses of 50–150 mg/g. The anti-Xa activity was selected as the biomarker, and the amidolytic assay method was validated and applied for pharmacokinetic studies of fucoidan. Fucoidan in OF penetrated the skin and distributed into the skin, striated muscle, and plasma with AUC0–48 = 0.94 μg·h/g, 2.22 μg·h/g, and 1.92 µg·h/mL, respectively. The longest half-life for fucoidan was observed in plasma, then in striated muscle and skin. It was found that the pharmacokinetics of fucoidan after topical OF application was linear, in the range of 50–150 mg/kg. No accumulation of fucoidan in plasma was observed after repeated topical applications of 100 mg/kg during five days. Our results support the rationality of topical application of formulations with fucoidan.
Potential Use of Seaweed Bioactive Compounds in Skincare—A Review
Authors: Jesumani Valentina , Du Hong , Aslam Muhammad , Pei Pengbing , Huang Nan
Published: December 2019

Modern lifestyles have developed new attention on appearance and personal care which attract a huge number of consumers towards cosmetic products. The demand for a skincare product with natural ingredients is rapidly increasing. Seaweeds are major resources for in-demand active compounds with a wide variety of applications. The use of seaweed-derived ingredients in cosmetic products has increased in recent years as many scientific studies have proved the potential skincare properties of seaweed bioactive compounds. This review emphasizes possible skincare properties of seaweed bioactive compounds. The review outlines the mechanism involved in skin problems including hyperpigmentation, premature skin aging, and acne in the first part while the second part focuses on the promising application of seaweeds in skin protection by highlighting the bioactive compound responsible for their bioactivity.