bluuebunny/crossref_metadata_2025 huggingface dataset:
Towards Wearable Augmented Reality in Healthcare: A Comparative Survey and Analysis of Head-Mounted Displays
Authors: Baashar Yahia , Alkawsi Gamal , Wan Ahmad Wan Nooraishya , Alomari Mohammad Ahmed , Alhussian Hitham , Tiong Sieh Kiong
Published: February 2023

Head-mounted displays (HMDs) have the potential to greatly impact the surgical field by maintaining sterile conditions in healthcare environments. Google Glass (GG) and Microsoft HoloLens (MH) are examples of optical HMDs. In this comparative survey related to wearable augmented reality (AR) technology in the medical field, we examine the current developments in wearable AR technology, as well as the medical aspects, with a specific emphasis on smart glasses and HoloLens. The authors searched recent articles (between 2017 and 2022) in the PubMed, Web of Science, Scopus, and ScienceDirect databases and a total of 37 relevant studies were considered for this analysis. The selected studies were divided into two main groups; 15 of the studies (around 41%) focused on smart glasses (e.g., Google Glass) and 22 (59%) focused on Microsoft HoloLens. Google Glass was used in various surgical specialities and preoperative settings, namely dermatology visits and nursing skill training. Moreover, Microsoft HoloLens was used in telepresence applications and holographic navigation of shoulder and gait impairment rehabilitation, among others. However, some limitations were associated with their use, such as low battery life, limited memory size, and possible ocular pain. Promising results were obtained by different studies regarding the feasibility, usability, and acceptability of using both Google Glass and Microsoft HoloLens in patient-centric settings as well as medical education and training. Further work and development of rigorous research designs are required to evaluate the efficacy and cost-effectiveness of wearable AR devices in the future.
Endometrial Injury Upregulates Expression of Receptivity Genes in Women with Implantation Failure
Authors: Celik Onder , Yurci Arzu , Ersahin Aynur , Gungor Nur D. , Celik Nilufer , Ozcil Mustafa D. , Dogan Serdar , Dalkilic Semih , Dalkilic Lutfiye , Ulug Ulun , Celik Sudenaz , Tinelli Andrea
Published: February 2023

Background: Homeobox genes A10 (HOXA10) and A11 (HOXA11), members of the abdominal B gene family, are responsible for embryonic survival and implantation. This study was planned to investigate whether endometrial injury alters the expression of both transcripts in women with implantation failure. Methods: A total of 54 women with implantation failure were divided into two equal groups as experimental (scratching) and sham (no scratching). Participants in the scratching group were exposed to endometrial injury in the mid-luteal phase, and those in the sham group were exposed to endometrial flushing. The scratching group, but not the sham group, underwent prior endometrial sampling. A second endometrial sampling was performed on the scratching group in the mid-luteal phase of the following cycle. The mRNA and protein levels of the HOXA10 and 11 transcripts were determined in endometrial samples collected before and after injury/flushing. Participants in each group underwent IVF/ET in the cycle after the second endometrial sampling. Results: Endometrial injury caused a 60.1-fold (p < 0.01) increase in HOXA10 mRNA and a 9.0-fold increase in HOXA11 mRNA (p < 0.02). Injury resulted in a significant increase in both HOXA10 (p < 0.001) and HOXA11 protein expression (p < 0.003). There was no significant change in HOXA10 and 11 mRNA expressions after flushing. Clinical pregnancy, live birth, and miscarriage rates of the both groups were similar. Conclusions: Endometrial injury increases homeobox transcript expression at both mRNA and protein levels.
Epidemiological Characteristics of Notifiable Respiratory Infectious Diseases in Mainland China from 2010 to 2018
Authors: Deng Lele , Han Yajun , Wang Jinlong , Liu Haican , Li Guilian , Wang Dayan , He Guangxue
Published: February 2023

Respiratory infectious diseases (RIDs) pose threats to people’s health, some of which are serious public health problems. The aim of our study was to explore epidemic situations regarding notifiable RIDs and the epidemiological characteristics of the six most common RIDs in mainland China. We first collected the surveillance data of all 12 statutory notifiable RIDs for 31 provinces in mainland China that reported between 2010 and 2018, and then the six most prevalent RIDs were selected to analyze their temporal, seasonal, spatiotemporal and population distribution characteristics. From 2010 to 2018, there were 13,985,040 notifiable cases and 25,548 deaths from RIDs in mainland China. The incidence rate of RIDs increased from 109.85/100,000 in 2010 to 140.85/100,000 in 2018. The mortality from RIDs ranged from 0.18/100,000 to 0.24/100,000. The most common RIDs in class B were pulmonary tuberculosis (PTB), pertussis, and measles, while those in class C were seasonal influenza, mumps and rubella. From 2010 to 2018, the incidence rate of PTB and rubella decreased; however, pertussis and seasonal influenza increased, with irregular changes in measles and mumps. The mortality from PTB increased from 2015 to 2018, and the mortality from seasonal influenza changed irregularly. PTB was mainly prevalent among people over 15 years old, while the other five common RIDs mostly occurred among people younger than 15 years old. The incidence of the six common RIDs mostly occurred in winter and spring, and they were spatiotemporally clustered in different areas and periods. In conclusion, PTB, seasonal influenza and mumps remain as public health problems in China, suggesting that continuous government input, more precise interventions, and a high-tech digital/intelligent surveillance and warning system are required to rapidly identify emerging events and timely response.
From Risk Assessment on Site to How to Improve Safety: An Easy “App” to Control Construction Site Conditions
Authors: Forteza Francisco J. , Carretero-Gómez José M. , Estudillo Barbara , Sesé Albert
Published: February 2023

A construction site has specific risks and organizational conditions requiring on-site safety inspections. Paperwork inspections have important limitations that can be overcome by substituting paper records with digital registers and using new information and communication technologies. Although academic literature has provided several tools to carry out on-site safety inspections adopting new technologies, most construction sites are not currently ready to adopt them. This paper covers this need of on-site control by providing an application that uses a simple technology accessible to most construction companies. The main objective and contribution of this paper is to design, develop, and implement a mobile device application (App), named “RisGES”. It is based on the model of risk that grounds the Construction Site Risk Assessment Tool (CONSRAT) and on the related models that connect risk with specific organizational and safety resources. This proposed application is aimed to assess the on-site risk and organizational structure by using new technologies and considering all relevant resources and material safety conditions. The paper includes practical examples of how to use RisGES in real settings. Evidence for the discriminant validity of CONSRAT is also provided. The RisGES tool is at once preventive and predictive since it yields a specific set of criteria for interventions intended to decrease the levels of risks on-site, as well as to detect improvement needs in the site structure and resources for increasing the safety levels.
The Prevalence of the Risk of Sexual Dysfunction in the First and Third Trimesters of Pregnancy in a Sample of Spanish Women
Authors: del Río Olvera Francisco Javier , Sánchez-Sandoval Yolanda , García-Rojas Antonio Daniel , Rodríguez-Vargas Susana , Ruiz-Ruiz Javier
Published: February 2023

Sexuality is a central aspect for all human beings. Research into the prevalence of sexual dysfunction in pregnant Spanish women is scarce. The aim of this work is to examine the prevalence of risk of sexual dysfunctions in pregnant Spanish women and determine in which trimester the greatest difficulties in sexual response occur. The sample consisted of 180 pregnant Spanish women, with an average age of 32.03 years (SD = 4.93). The participants completed a questionnaire for socio-demographic data, as well as the female sexual function index, the state/trait depression inventory, and the dyadic adjustment scale. The results indicate that the percentage of women with a risk of sexual dysfunction was 65% in the first trimester and 81.11% in the third trimester. Likewise, the highest score on the depression questionnaire was in the third trimester, and the couple’s relationship also improved in the third trimester. To improve women’s sex lives during pregnancy, it is recommended to increase sexual education and information for both pregnant women and their partners.
Post-Disaster Restoration and Reconstruction Assessment of the Jiuzhaigou Lake Landscape and a Resilience Development Pathway
Authors: Zhao Liang , Xu Gaofeng , Cui Yan , Kong Feng , Gao Huina , Zhou Xia
Published: February 2023

The essence of post-disaster reconstruction is the restoration and rebirth of the affected areas. The earthquake hitting Jiuzhaigou was the first earthquake that had its epicenter in the World Natural Heritage located in China. Ecological restoration and landscape reconstruction are essential for the sustainable development of tourism. This study uses high-resolution remote sensing images to monitor and evaluate the post-disaster restoration and reconstruction process of the leading lakes in Jiuzhaigou. It was found that the lake water quality, vegetation, and road facilities have undergone moderate reconstruction. However, the restoration and reconstruction still faced severe challenges. The ecological environment’s stability and balance are prerequisites for the sustainable development of the World Natural Heritage sites. This paper combines the “Build Back Better” concept that advocates risk reduction, scenic spot restoration, and efficient implementation to ensure Jiuzhaigou’s restoration and sustainable development. It comes up with specific measures for the resilience development of Jiuzhaigou from the eight principles of overall planning, structural resilience, disaster prevention and mitigation, landscape facilities, social psychology, management mechanisms, policies and regulations, and monitoring and evaluation to provide a reference for the sustainable development of tourism.
Exploring the Influencing Factors of COVID-19 Vaccination Willingness among Young Adults in China
Authors: Su Yue , Li Sijia , Huang Feng , Xue Jia , Zhu Tingshao
Published: February 2023

Vaccine uptake is considered as one of the most effective methods of defending against COVID-19 (coronavirus disease 2019). However, many young adults are hesitant regarding COVID-19 vaccines, and they actually play an important role in virus transmission. Based on a multi-theory model, this study aims to explore the influencing factors related to COVID-19 vaccine willingness among young adults in China. Using semi-structured interviews, this study explored the factors that would motivate young adults with vaccine hesitancy to get the COVID-19 vaccine. Thematic analysis was used to analyze the interview data with topic modeling as a complementarity method. After comparing the differences and similarities of results generated by thematic analysis and topic modeling, this study ultimately identified ten key factors related to COVID-19 vaccination intention, including the effectiveness and safety of vaccines, application range of vaccine, etc. This study combined thematic analysis with machine learning and provided a comprehensive and nuanced picture of facilitating factors for COVID-19 vaccine uptake among Chinese young adults. Results may be taken as potential themes for authorities and public health workers in vaccination campaigns.
Visibility as a Key Dimension to Better Health-Related Quality of Life and Mental Health: Results of the European Union Funded “ME-WE” Online Survey Study on Adolescent Young Carers in Switzerland
Authors: Guggiari Elena , Fatton Marianne , Becker Saul , Lewis Feylyn , Casu Giulia , Hoefman Renske , Hanson Elizabeth , Santini Sara , Boccaletti Licia , Nap Henk Herman , Hlebec Valentina , Wirth Alexandra , Leu Agnes
Published: February 2023

This paper examines the health-related quality of life (HRQL) and mental health of adolescent young carers (AYCs) aged 15–17 in Switzerland, based on data collected within the Horizon 2020 project ‘Psychosocial support for promoting mental health and well-being among AYCs in Europe’ (ME-WE). It addresses the following questions: (1) Which characteristics of AYCs are associated with lower HRQL and with higher level of mental health problems? (2) Do AYCs who are less visible and less supported report a lower HRQL and more mental health issues than other AYCs? A total of 2343 young people in Switzerland, amongst them 240 AYCs, completed an online survey. The results show that female AYCs and AYCs with Swiss nationality more often reported having mental health issues than their male and non-Swiss counterparts. Furthermore, the findings show a significant association between receiving support for themselves and visibility from their school or employer and the HRQL. Moreover, AYCs who reported that their school or employer knew about the situation also reported fewer mental health issues. These findings can inform recommendations for policy and practice to develop measures aimed at raising the visibility of AYCs, which is the first step for planning AYC tailored support.
Estimating Depressive Symptom Class from Voice
Authors: Takano Takeshi , Mizuguchi Daisuke , Omiya Yasuhiro , Higuchi Masakazu , Nakamura Mitsuteru , Shinohara Shuji , Mitsuyoshi Shunji , Saito Taku , Yoshino Aihide , Toda Hiroyuki , Tokuno Shinichi
Published: February 2023

Voice-based depression detection methods have been studied worldwide as an objective and easy method to detect depression. Conventional studies estimate the presence or severity of depression. However, an estimation of symptoms is a necessary technique not only to treat depression, but also to relieve patients’ distress. Hence, we studied a method for clustering symptoms from HAM-D scores of depressed patients and by estimating patients in different symptom groups based on acoustic features of their speech. We could separate different symptom groups with an accuracy of 79%. The results suggest that voice from speech can estimate the symptoms associated with depression.
The Impact of Women’s Agency on Accessing and Using Maternal Healthcare Services: A Systematic Review and Meta-Analysis
Authors: Vizheh Maryam , Rapport Frances , Braithwaite Jeffrey , Zurynski Yvonne
Published: February 2023

Agency, defined as the ability to identify one’s goals and act upon them, has been recognized as a prominent strategy to access maternal healthcare services (MHS). The purpose of this study was to synthesize evidence of the association between women’s agency and MHS utilization. A systematic review was performed on five academic databases, comprising Scopus, PubMed, Web of Science, Embase, and ProQuest. Meta-analysis was performed with a random-effects method using the STATA™ Version 17 software. A total of 82 studies were selected following the PRISMA guidelines. The meta-analysis demonstrated that an increase in women’s agency was associated with a 34% increase in the odds of receiving skilled antenatal care (ANC) (OR = 1.34, 95% CI = 1.18–1.52); 7% increase in the odds of initiating the first ANC visit during the first trimester of pregnancy (OR = 1.07, 95% CI = 1.01–1.12); 20% increase in the odds of receiving at least one ANC visit (OR = 1.20, 95% CI = 1.04–1.4); 16% increase in the odds of receiving more than four ANC visits during pregnancy (OR = 1.16, 95% CI = 1.12–1.21); 17% increase in the odds of receiving more than eight ANC visits (OR = 1.17, 95% CI = 1.04–1.32); 13% increase in the odds of facility-based delivery (OR = 1.13, 95% CI = 1.09–1.17); 16% increase in the odds of using skilled birth attendants (OR = 1.16, 95% CI = 1.13–1.19); and 13% increase in the odds of receiving postnatal care (OR = 1.13, 95% CI = 1.08–1.19) compared to low level of agency. Any efforts to improve MHS utilization and reduce maternal morbidity and mortality should include the promotion of women’s agency.
Environmental Health Knowledge Does Not Necessarily Translate to Action in Youth
Authors: Elshaer Shereen , Martin Lisa J. , Baker Theresa A. , Roberts Erin , Rios-Santiago Paola , Kaufhold Ross , Butsch Kovacic Melinda
Published: February 2023

Environmental challenges pose serious health problems, especially for children, and lay public action is lacking. This study sought to characterize the relationship between environmental health knowledge and behavior in youth. A cross-sectional, descriptive survey with quantitative and qualitative questions was conducted. Open-ended questions were coded to generate themes/subthemes. Subscales’ scores were presented as mean ± SD or median and interquartile range (IQR). T- and Mann–Whitney tests were used to compare groups, and correlations were used to evaluate covariation. A total of 452 children were surveyed. Youth verbalized concerns about their environments and their impact on health. Air pollution was the most concerning issue. Participants had moderate knowledge scores. Few described the three health domains; even fewer included environment. Behavior scores were low and weakly correlated with knowledge, but were moderately correlated with attitude and self-efficacy. Participation in environmental classes, activities, and clubs was associated with higher scores. We found variable environmental health knowledge, limited understanding of the local environment’s impact on health, and a weak association between youth’s knowledge and behavior. Focused formal and non-formal educational experiences were associated with improved scores, indicating the value of targeted youth educational programming to increase environmental health knowledge and action.
The Development of Emotion Understanding among Five- and Six-Year-Old Left-Behind Children in Rural China
Authors: Tan Ruifeng , Fang Huimin , Chen Suiqing
Published: February 2023

The left-behind children (LBC), separated from their mother/father or parents for a long period of time, have long been discussed as a subject of concern in China. Existing research has concluded that rural children who did not migrate with parents are subject to emotional risks. In the present study, the purpose is to study the impact of parental migration on early emotional understanding. Purposeful sampling was used to recruit 180 children aged five to six years in rural areas of Guangdong province, including LBC and non-left-behind children (NLBC). Their level of emotional understanding (EU) was assessed by the emotional comprehension test (TEC) adapted to the Chinese context. The results showed that, on the three levels (External, Internal, Reflective) of emotional understanding, LBC aged five- to six- years old scored significantly lower than NLBC as counterparts. On the whole, the emotional comprehension ability of preschool LBC was significantly lower than that of NLBC. However, there were no significant differences within LBC nurtured by single parents, grandparents, and other relatives. This study confirmed that parental migration in early childhood considerably impacted rural LBC’s emotional understanding and affectional adjustment, which provided a significant basis for increasing parental care and early childhood companionship in rural areas.
Socioeconomic Factors Influence the Spatial and Temporal Distribution of Blue–Green Infrastructure Demand: A Case of Nanjing City
Authors: Zhao Haixia , Gu Binjie , Fan Jinding , Wang Junqi , Luo Liancong
Published: February 2023

Blue–green infrastructure provides a variety of ecosystem services and is becoming an increasingly vital part of urban ecosystem protection. It is an ecological facility for ecological conservation and environmental protection, and a foundation for realizing people’s needs for a better life. This study selects indicators from four dimensions: social, economic, environmental, and ecological, and the demand for blue–green infrastructure is assessed comprehensively. The results show that: (1) the demand for blue–green infrastructure varies spatially with the development of the city; (2) the total demand for blue–green infrastructure in Nanjing from 2000 to 2020 shows a pattern of “high in the center and low in the periphery”; (3) the level of economic development, urban spatial pattern, and decision management orientation have different degrees of influence on the demand for blue–green infrastructure, with the urban spatial pattern having the greatest impact. Therefore, in the future, blue–green infrastructure should be optimized by taking into account the spatial characteristics of demand in Nanjing.
Holistic Framework to Contextualize Dietary Quality Assessment: A Critical Review
Authors: Phelan Jessica M. , Rosenkranz Richard R. , Phelan Connor J. , Rosenkranz Sara K.
Published: February 2023

Numerous dietary quality indices exist to help quantify overall dietary intake and behaviors associated with positive health outcomes. Most indices focus solely on biomedical factors and nutrient or food intake, and exclude the influence of important social and environmental factors associated with dietary intake. Using the Diet Quality Index- International as one sample index to illustrate our proposed holistic conceptual framework, this critical review seeks to elucidate potential adaptations to dietary quality assessment by considering—in parallel—biomedical, environmental, and social factors. Considering these factors would add context to dietary quality assessment, influencing post-assessment recommendations for use across various populations and circumstances. Additionally, individual and population-level evidence-based practices could be informed by contextual social and environmental factors that influence dietary quality to provide more relevant, reasonable, and beneficial nutritional recommendations.
Nutritional Content of Popular Menu Items from Online Food Delivery Applications in Bangkok, Thailand: Are They Healthy?
Authors: Jindarattanaporn Nongnuch , Suya Inthira , Lorenzetti Lara , Kantachuvesiri Surasak , Thamarangsi Thaksaphon
Published: February 2023

The rise in online food delivery (OFD) applications has increased access to a myriad of ready-to-eat options, which may lead to unhealthier food choices. Our objective was to assess the nutritional profile of popular menu items available through OFD applications in Bangkok, Thailand. We selected the top 40 popular menu items from three of the most commonly used OFD applications in 2021. Each menu item was collected from the top 15 restaurants in Bangkok for a total of 600 items. Nutritional contents were analysed by a professional food laboratory in Bangkok. Descriptive statistics were employed to describe the nutritional content of each menu item, including energy, fat, sodium, and sugar content. We also compared nutritional content to the World Health Organization’s recommended daily intake values. The majority of menu items were considered unhealthy, with 23 of the 25 ready-to-eat menu items containing more than the recommended sodium intake for adults. Eighty percent of all sweets contained approximately 1.5 times more sugar than the daily recommendation. Displaying nutrition facts in the OFD applications for menu items and providing consumers with filters for healthier options are required to reduce overconsumption and improve consumer food choice.
Exploring the Reported Strengths and Limitations of Aboriginal and Torres Strait Islander Health Research: A Narrative Review of Intervention Studies
Authors: McGuffog Romany , Bryant Jamie , Booth Kade , Collis Felicity , Brown Alex , Hughes Jaquelyne T. , Chamberlain Catherine , McGhie Alexandra , Hobden Breanne , Kennedy Michelle
Published: February 2023

High quality intervention research is needed to inform evidence-based practice and policy for Aboriginal and Torres Strait Islander communities. We searched for studies published from 2008–2020 in the PubMed database. A narrative review of intervention literature was conducted, where we identified researcher reported strengths and limitations of their research practice. A total of 240 studies met inclusion criteria which were categorised as evaluations, trials, pilot interventions or implementation studies. Reported strengths included community engagement and partnerships; sample qualities; Aboriginal and Torres Strait Islander involvement in research; culturally appropriate and safe research practice; capacity building efforts; providing resources or reducing costs for services and communities; understanding local culture and context; and appropriate timelines for completion. Reported limitations included difficulties achieving the target sample size; inadequate time; insufficient funding and resources; limited capacity of health workers and services; and inadequate community involvement and communication issues. This review highlights that community consultation and leadership coupled with appropriate time and funding, enables Aboriginal and Torres Strait Islander health intervention research to be conducted. These factors can enable effective intervention research, and consequently can help improve health and wellbeing outcomes for Aboriginal and Torres Strait Islander people.
Study on Spatiotemporal Evolution Features and Affecting Factors of Collaborative Governance of Pollution Reduction and Carbon Abatement in Urban Agglomerations of the Yellow River Basin
Authors: Su Zhaoxian , Yang Yang , Wang Yun , Zhang Pan , Luo Xin
Published: February 2023

Exploring spatiotemporal evolution features and factors affecting pollution reduction and carbon abatement on the urban agglomeration scale is helpful to better understand the interaction between ecological environment and economic development in urban agglomerations. In this study, we constructed an evaluation index system for collaborative governance of pollution reduction and carbon abatement in urban agglomerations. In addition, we employed the correlation coefficient matrix, the composite system synergy model, the Gini coefficient, and the Theil index to evaluate the level of and regional differences in collaborative governance of pollution reduction and carbon abatement in seven urban agglomerations in the Yellow River Basin from 2006 to 2020. Moreover, we explored the factors affecting collaborative governance of pollution reduction and carbon abatement in urban agglomerations in the basin. The following findings were obtained: (1) the order degree of collaborative governance of pollution reduction and carbon abatement in the seven urban agglomerations exhibited a significant growing trend, representing a spatial evolution feature of “high in the west and low in the east”; (2) the internal differences in collaborative governance synergy of pollution reduction and carbon abatement decreased in Lanzhou–Xining Urban Agglomeration, Hohhot–Baotou–Ordos–Yulin Urban Agglomeration, Central Shanxi Urban Agglomeration, Zhongyuan Urban Agglomeration, and Shandong Peninsula Urban Agglomeration, while the internal differences basically remained stable in Guanzhong Urban Agglomeration and the Urban Agglomeration along the Yellow River in Ningxia; (3) the variances in environmental regulation and industrial structure among urban agglomerations had a significant positive effect on collaborative governance of pollution reduction and carbon abatement in urban agglomerations in the basin, and the variances in economic growth had a significant inhibitory effect. In addition, the variances in energy consumption, greening construction, and opening-up had an inhibitory impact on collaborative governance of pollution reduction, but the impact was not significant. Finally, this study proposes various recommendations to improve collaborative governance for pollution reduction and carbon abatement in urban agglomerations in the basin in terms of promoting industrial structure upgrading, strengthening regional cooperation, and reducing regional differences. This paper represents an empirical reference for formulating differentiated collaborative governance strategies for pollution reduction and carbon abatement, comprehensive green and low-carbon economic and social transformation programs, and high-quality green development paths in urban agglomerations, which is of certain theoretical and practical significance.
Factors Related to Physical Activity among Older Adults Who Relocated to a New Community after the Kumamoto Earthquake: A Study from the Viewpoint of Social Capital
Authors: Kanamori Yumie , Ide-Okochi Ayako , Samiso Tomonori
Published: February 2023

Previous studies have shown an association between social capital and physical activity in older adults. Older adults who relocated after the Kumamoto earthquake may become physically inactive, and the extent of this inactivity may be buffered by social capital. Accordingly, this study applied the social capital perspective to examine factors that affect the physical activity of older adults who relocated to a new community after the Kumamoto earthquake. We conducted a self-administered mail questionnaire survey with 1494 (613 male, 881 female, mean age 75.12 ± 7.41 years) evacuees from temporary housing in Kumamoto City, aged 65 years and above, who relocated to a new community after the earthquake. We performed a binomial logistic regression to examine the factors affecting participants’ physical activity. The results showed that physical inactivity (decreased opportunities for physical activity, decreased walking speed, and no exercise habits) was significantly associated with non-participation in community activities, lack of information about community activities, and being aged 75 years and over. Lack of social support from friends was significantly associated with lack of exercise habits. These findings encourage participation in community activities, alongside giving and receiving social support in health activities that target older adults who relocated to new communities after the earthquake.
Theoretically Revealing the Response of Intermolecular Vibration Energy Transfer and Decomposition Process of the DNTF System to Electric Fields Using Two-Dimensional Infrared Spectra
Authors: Ren Haichao , Ji Linxiang , Jia Xianzhen , Tao Jun , Liu Ruipeng , Wei Dongqing , Wang Xiaofeng , Ji Guangfu
Published: February 2023

The external electric field (E-field), which is an important stimulus, can change the decomposition mechanism and sensitivity of energetic materials. As a result, understanding the response of energetic materials to external E-fields is critical for their safe use. Motivated by recent experiments and theories, the two-dimensional infrared (2D IR) spectra of 3,4-bis (3-nitrofurazan-4-yl) furoxan (DNTF), which has a high energy, a low melting point, and comprehensive properties, were theoretically investigated. Cross-peaks were observed in 2D IR spectra under different E-fields, which demonstrated an intermolecular vibration energy transfer; the furazan ring vibration was found to play an important role in the analysis of vibration energy distribution and was extended over several DNTF molecules. Measurements of the non-covalent interactions, with the support of the 2D IR spectra, indicated that there were obvious non-covalent interactions among different DNTF molecules, which resulted from the conjugation of the furoxan ring and the furazan ring; the direction of the E-field also had a significant influence on the strength of the weak interactions. Furthermore, the calculation of the Laplacian bond order, which characterized the C-NO2 bonds as trigger bonds, predicted that the E-fields could change the thermal decomposition process of DNTF while the positive E-field facilitates the breakdown of the C-NO2 in DNTFⅣ molecules. Our work provides new insights into the relationship between the E-field and the intermolecular vibration energy transfer and decomposition mechanism of the DNTF system.
Photosynthesis 2.0
Authors: Cazzaniga Stefano , Ballottari Matteo
Published: February 2023

Photosynthesis is a process that provides the continuous income of energy needed to sustain life on our planet [...]
Optic Nerve Injury Enhanced Mitochondrial Fission and Increased Mitochondrial Density without Altering the Uniform Mitochondrial Distribution in the Unmyelinated Axons of Retinal Ganglion Cells in a Mouse Model
Authors: Tsuji Takahiro , Murase Tomoya , Konishi Yoshiyuki , Inatani Masaru
Published: February 2023

Glaucomatous optic neuropathy (GON), a major cause of blindness, is characterized by the loss of retinal ganglion cells (RGCs) and the degeneration of their axons. Mitochondria are deeply involved in maintaining the health of RGCs and their axons. Therefore, lots of attempts have been made to develop diagnostic tools and therapies targeting mitochondria. Recently, we reported that mitochondria are uniformly distributed in the unmyelinated axons of RGCs, possibly owing to the ATP gradient. Thus, using transgenic mice expressing yellow fluorescent protein targeting mitochondria exclusively in RGCs within the retina, we assessed the alteration of mitochondrial distributions induced by optic nerve crush (ONC) via in vitro flat-mount retinal sections and in vivo fundus images captured with a confocal scanning ophthalmoscope. We observed that the mitochondrial distribution in the unmyelinated axons of survived RGCs after ONC remained uniform, although their density increased. Furthermore, via in vitro analysis, we discovered that the mitochondrial size is attenuated following ONC. These results suggest that ONC induces mitochondrial fission without disrupting the uniform mitochondrial distribution, possibly preventing axonal degeneration and apoptosis. The in vivo visualization system of axonal mitochondria in RGCs may be applicable in the detection of the progression of GON in animal studies and potentially in humans.
CsgI (YccT) Is a Novel Inhibitor of Curli Fimbriae Formation in Escherichia coli Preventing CsgA Polymerization and Curli Gene Expression
Authors: Sano Kotaro , Kobayashi Hiroaki , Chuta Hirotaka , Matsuyoshi Nozomi , Kato Yuki , Ogasawara Hiroshi
Published: February 2023

Curli fimbriae are amyloids—found in bacteria (Escherichia coli)—that are involved in solid-surface adhesion and bacterial aggregation during biofilm formation. The curli protein CsgA is coded by a csgBAC operon gene, and the transcription factor CsgD is essential to induce its curli protein expression. However, the complete mechanism underlying curli fimbriae formation requires elucidation. Herein, we noted that curli fimbriae formation was inhibited by yccT—i.e., a gene that encodes a periplasmic protein of unknown function regulated by CsgD. Furthermore, curli fimbriae formation was strongly repressed by CsgD overexpression caused by a multicopy plasmid in BW25113—the non-cellulose-producing strain. YccT deficiency prevented these CsgD effects. YccT overexpression led to intracellular YccT accumulation and reduced CsgA expression. These effects were addressed by deleting the N-terminal signal peptide of YccT. Localization, gene expression, and phenotypic analyses revealed that YccT-dependent inhibition of curli fimbriae formation and curli protein expression was mediated by the two-component regulatory system EnvZ/OmpR. Purified YccT inhibited CsgA polymerization; however, no intracytoplasmic interaction between YccT and CsgA was detected. Thus, YccT—renamed CsgI (curli synthesis inhibitor)—is a novel inhibitor of curli fimbriae formation and has a dual role as an OmpR phosphorylation modulator and CsgA polymerization inhibitor.
Recent Advances and Progress on Melanin: From Source to Application
Authors: Guo Lili , Li Wenya , Gu Zhiyang , Wang Litong , Guo Lan , Ma Saibo , Li Cuiyao , Sun Jishang , Han Baoqin , Chang Jing
Published: February 2023

Melanin is a biological pigment formed by indoles and phenolic compounds. It is widely found in living organisms and has a variety of unique properties. Due to its diverse characteristics and good biocompatibility, melanin has become the focus in the fields of biomedicine, agriculture, the food industry, etc. However, due to the wide range of melanin sources, complex polymerization properties, and low solubility of specific solvents, the specific macromolecular structure and polymerization mechanism of melanin remain unclear, which significantly limits the further study and application of melanin. Its synthesis and degradation pathways are also controversial. In addition, new properties and applications of melanin are constantly being discovered. In this review, we focus on the recent advances in the research of melanin in all aspects. Firstly, the classification, source, and degradation of melanin are summarized. Secondly, a detailed description of the structure, characterization, and properties of melanin is followed. The novel biological activity of melanin and its application is described at the end.
1α,25(OH)2D3 Promotes the Autophagy of Porcine Ovarian Granulosa Cells as a Protective Mechanism against ROS through the BNIP3/PINK1 Pathway
Authors: Wang Shiyou , Yao Qichun , Zhao Fan , Cui Wenfei , Price Christopher A. , Wang Yifan , Lv Jing , Tang Hong , Jiang Zhongliang
Published: February 2023

Vitamin D (VD) is one of the important nutrients required by livestock; however, VD deficiency is reported to be widespread. Earlier studies have suggested a potential role for VD in reproduction. Studies on the correlation between VD and sow reproduction are limited. The aim of the current study was aimed to determine the role of 1,25-dihydroxy vitamin D3 (1α,25(OH)2D3) on porcine ovarian granulosa cells (PGCs) in vitro to provide a theoretical basis for improving the reproductive efficiency of sows. We used chloroquine (autophagy inhibitor) and reactive oxygen species (ROS) scavenger N-acetylcysteine in conjunction with 1α,25(OH)2D3 to explore the effect on PGCs. The results showed that 10 nM of 1α,25(OH)2D3 increased PGC viability and ROS content. In addition, 1α,25(OH)2D3 induces PGC autophagy according to the gene transcription and protein expression levels of LC3, ATG7, BECN1, and SQSTM1 and promotes the generation of autophagosomes. 1α,25(OH)2D3-induced autophagy affects the synthesis of E2 and P4 in PGCs. We investigated the relationship between ROS and autophagy, and the results showed that 1α,25(OH)2D3-induced ROS promoted PGC autophagy. The ROS-BNIP3-PINK1 pathway was involved in PGC autophagy induced by 1α,25(OH)2D3. In conclusion, this study suggests that 1α,25(OH)2D3 promotes PGC autophagy as a protective mechanism against ROS via the BNIP3/PINK1 pathway.
Co-Transplantation of Barcoded Lymphoid-Primed Multipotent (LMPP) and Common Lymphocyte (CLP) Progenitors Reveals a Major Contribution of LMPP to the Lymphoid Lineage
Authors: Michaels Victoria , Chalabi Smahane , Legrand Agnes , Renard Julie , Tejerina Emmanuel , Daouya Marina , Fabrega Sylvie , Megret Jérôme , Olaso Robert , Boland Anne , Deleuze Jean-François , Battail Christophe , Tronik-Le Roux Diana , Ezine Sophie
Published: February 2023

T cells have the potential to maintain immunological memory and self-tolerance by recognizing antigens from pathogens or tumors. In pathological situations, failure to generate de novo T cells causes immunodeficiency resulting in acute infections and complications. Hematopoietic stem cells (HSC) transplantation constitutes a valuable option to restore proper immune function. However, delayed T cell reconstitution is observed compared to other lineages. To overcome this difficulty, we developed a new approach to identify populations with efficient lymphoid reconstitution properties. To this end, we use a DNA barcoding strategy based on the insertion into a cell chromosome of a lentivirus (LV) carrying a non-coding DNA fragment named barcode (BC). These will segregate through cell divisions and be present in cells’ progeny. The remarkable characteristic of the method is that different cell types can be tracked simultaneously in the same mouse. Thus, we in vivo barcoded LMPP and CLP progenitors to test their ability to reconstitute the lymphoid lineage. Barcoded progenitors were co-grafted in immuno-compromised mice and their fate analyzed by evaluating the BC composition in transplanted mice. The results highlight the predominant role of LMPP progenitors for lymphoid generation and reveal valuable novel insights to be reconsidered in clinical transplantation assays.
Evaluating the Targeting of a Staphylococcus-aureus-Infected Implant with a Radiolabeled Antibody In Vivo
Authors: van Dijk Bruce , Hooning van Duyvenbode J. Fred F. , de Vor Lisanne , Nurmohamed F. Ruben H. A. , Lam Marnix G. E. H. , Poot Alex J. , Ramakers Ruud M. , Koustoulidou Sofia , Beekman Freek J. , van Strijp Jos , Rooijakkers Suzan H. M. , Dadachova Ekaterina , Vogely H. Charles , Weinans Harrie , van der Wal Bart C. H.
Published: February 2023

Implant infections caused by Staphylococcus aureus are difficult to treat due to biofilm formation, which complicates surgical and antibiotic treatment. We introduce an alternative approach using monoclonal antibodies (mAbs) targeting S. aureus and provide evidence of the specificity and biodistribution of S.-aureus-targeting antibodies in a mouse implant infection model. The monoclonal antibody 4497-IgG1 targeting wall teichoic acid in S. aureus was labeled with indium-111 using CHX-A”-DTPA as a chelator. Single Photon Emission Computed Tomography/computed tomographyscans were performed at 24, 72 and 120 h after administration of the 111In-4497 mAb in Balb/cAnNCrl mice with a subcutaneous implant that was pre-colonized with S. aureus biofilm. The biodistribution of this labelled antibody over various organs was visualized and quantified using SPECT/CT imaging, and was compared to the uptake at the target tissue with the implanted infection. Uptake of the 111In-4497 mAbs at the infected implant gradually increased from 8.34 %ID/cm3 at 24 h to 9.22 %ID/cm3 at 120 h. Uptake at the heart/blood pool decreased over time from 11.60 to 7.58 %ID/cm3, whereas the uptake in the other organs decreased from 7.26 to less than 4.66 %ID/cm3 at 120 h. The effective half-life of 111In-4497 mAbs was determined to be 59 h. In conclusion, 111In-4497 mAbs were found to specifically detect S. aureus and its biofilm with excellent and prolonged accumulation at the site of the colonized implant. Therefore, it has the potential to serve as a drug delivery system for the diagnostic and bactericidal treatment of biofilm.
Cytokinin Response Factor 9 Represses Cytokinin Responses in Flower Development
Authors: Swinka Christine , Hellmann Eva , Zwack Paul , Banda Ramya , Rashotte Aaron M. , Heyl Alexander
Published: February 2023

A multi-step phosphorelay system is the main conduit of cytokinin signal transduction. However, several groups of additional factors that also play a role in this signaling pathway have been found—among them the Cytokinin Response Factors (CRFs). In a genetic screen, CRF9 was identified as a regulator of the transcriptional cytokinin response. It is mainly expressed in flowers. Mutational analysis indicates that CRF9 plays a role in the transition from vegetative to reproductive growth and silique development. The CRF9 protein is localized in the nucleus and functions as a transcriptional repressor of Arabidopsis Response Regulator 6 (ARR6)—a primary response gene for cytokinin signaling. The experimental data suggest that CRF9 functions as a repressor of cytokinin during reproductive development.
Sphingolipid Long-Chain Base Signaling in Compatible and Non-Compatible Plant–Pathogen Interactions in Arabidopsis
Authors: Saucedo-García Mariana , González-Solís Ariadna , Rodríguez-Mejía Priscila , Lozano-Rosas Guadalupe , Olivera-Flores Teresa de Jesús , Carmona-Salazar Laura , Guevara-García A. Arturo , Cahoon Edgar B. , Gavilanes-Ruíz Marina
Published: February 2023

The chemical diversity of sphingolipids in plants allows the assignment of specific roles to special molecular species. These roles include NaCl receptors for glycosylinositolphosphoceramides or second messengers for long-chain bases (LCBs), free or in their acylated forms. Such signaling function has been associated with plant immunity, with an apparent connection to mitogen-activated protein kinase 6 (MPK6) and reactive oxygen species (ROS). This work used in planta assays with mutants and fumonisin B1 (FB1) to generate varying levels of endogenous sphingolipids. This was complemented with in planta pathogenicity tests using virulent and avirulent Pseudomonas syringae strains. Our results indicate that the surge of specific free LCBs and ceramides induced by FB1 or an avirulent strain trigger a biphasic ROS production. The first transient phase is partially produced by NADPH oxidase, and the second is sustained and is related to programmed cell death. MPK6 acts downstream of LCB buildup and upstream of late ROS and is required to selectively inhibit the growth of the avirulent but not the virulent strain. Altogether, these results provide evidence that a LCB– MPK6– ROS signaling pathway contributes differentially to the two forms of immunity described in plants, upregulating the defense scheme of a non-compatible interaction.
Editorial of Special Issue “Protective and Detrimental Role of Heme Oxygenase-1”: 2021
Authors: Sorrenti Valeria
Published: February 2023

The Special Issue “Protective and detrimental role of heme oxygenase-1”(2021), in the International Journal of Molecular Sciences, includes original research papers and reviews aiming to understand the protective or detrimental role of HO-1 and the signaling pathway involved [...]
In Vitro Evaluation of Antioxidant and Protective Potential of Kombucha-Fermented Black Berry Extracts against H2O2-Induced Oxidative Stress in Human Skin Cells and Yeast Model
Authors: Ziemlewska Aleksandra , Zagórska-Dziok Martyna , Nizioł-Łukaszewska Zofia , Kielar Patrycja , Mołoń Mateusz , Szczepanek Dariusz , Sowa Ireneusz , Wójciak Magdalena
Published: February 2023

The fruits of R. nigrum L., A. melanocarpa Michx., and V. myrtillus L. are well-known natural plant materials with proven antioxidant activity. This work attempts to compare the antioxidant properties of extracts of these plants and ferments obtained during their fermentation using a consortium of microorganisms referred to as kombucha. As part of the work, a phytochemical analysis of extracts and ferments was carried out using the UPLC-MS method and the content of the main components was determined. The antioxidant properties of the tested samples and their cytotoxicity were assessed with the use of DPPH and ABTS radicals. The protective effect against hydrogen peroxide-induced oxidative stress was also assessed. The possibility of inhibiting the increase in the intracellular level of reactive oxygen species was carried out on both human skin cells (keratinocytes and fibroblasts) and the yeast Saccharomyces cerevisiae (wild-type strains and sod1Δ deletion mutants). The conducted analyses showed that the ferments obtained are characterized by a greater variety of biologically active compounds; in most cases they do not cause a cytotoxic effect, show strong antioxidant properties, and can reduce oxidative stress in both human and yeast cells. This effect depends on the concentration used and the fermentation time. The results obtained indicate that the tested ferments can be considered as an extremely valuable raw material protecting cells against the negative effects of oxidative stress.