bluuebunny/crossref_metadata_2025 huggingface dataset:
Development and Method Validation of Butyric Acid and Milk Fat Analysis in Butter Blends and Blended Milk Products by GC-FID
Authors: Danudol Arunee , Judprasong Kunchit
Published: November 2022

Butyric acid is a short-chain saturated fatty acid with four carbon atoms in its molecule. It is unique to butter made from cow’s milk and is an indicator to evaluate the quality of butter and milk products as stated in their ingredient labels. This study determined the milk fat content of butter blends and blended milk products by analyzing the content of butyric acid prepared as derivatives of methyl esters prior to injection into a gas chromatography flame ionization detector (GC–FID). Results revealed that this method had specificity, a linear relationship for concentration in the range of 0.04–1.60 mg/mL, a coefficient of determination (R2) > 0.999, an instrumental limit of detection (LOD) and a limit of quantitative analysis (LOQ) at 0.01% and 0.10% of total fat, respectively, and an instrumental working range of 0.10–3.60% of total fat. The results of a precision study using relative standard deviation (RSD) was 1.3%, while an accuracy study using the spiking method showed % recovery in the range of 98.2–101.9%. The method linearity range for milk fat analysis had a good linear correlation in the range of 3–100% of total fat (R2 > 0.999). Results for method LOD and LOQ were 1% and 3% of total fat, respectively. This method also had good precision (1.3% RSD) and accuracy (99.6–100.1% recovery), which indicates reliability in terms of precision and accuracy. This method, therefore, can be used to check claims about the quality of blended butter and blended milk products to ensure consumer confidence in product quality.
A Review of the Challenges Facing Global Commercialization of the Artificial Meat Industry
Authors: Liu Weijun , Hao Zhipeng , Florkowski Wojciech J. , Wu Linhai , Yang Zhengyong
Published: November 2022

The sustained growth of global meat consumption incentivized the development of the meat substitute industry. However, long-term global commercialization of meat substitutes faces challenges that arise from technological innovation, limited consumer awareness, and an imperfect regulatory environment. Many important questions require urgent answers. This paper presents a review of issues affecting meat substitute manufacturing and marketing, and helps to bridge important gaps which appear in the literature. To date, global research on meat substitutes focuses mainly on technology enhancement, cost reduction, and commercialization with a few studies focused on a regulatory perspective. Furthermore, the studies on meat substitute effects on environmental pollution reduction, safety, and ethical risk perception are particularly important. A review of these trends leads to conclusions which anticipate the development of a much broader market for the meat substitute industry over the long term, the gradual discovery of solutions to technical obstacles, upgraded manufacturing, the persistent perception of ethical risk and its influence on consumer willingness to accept meat substitutes, and the urgent need for constructing an effective meat substitute regulatory system.
Arbuscular Mycorrhizal Fungi Increase Nutritional Quality of Soilless Grown Lettuce while Overcoming Low Phosphorus Supply
Authors: Cela Fatjon , Avio Luciano , Giordani Tommaso , Vangelisti Alberto , Cavallini Andrea , Turrini Alessandra , Sbrana Cristiana , Pardossi Alberto , Incrocci Luca
Published: November 2022

Lettuce is widely used for its healthy properties, and it is of interest to increase them with minimal environmental impact. The purpose of this work was to evaluate the effect of the arbuscular mycorrhizal fungus (AMF) Funneliformis mosseae in lettuce plants (Lactuca sativa L. cv. Salinas) cultivated in a soilless system with sub-optimal phosphorus (P) compared with non-inoculated controls at two different P concentrations. Results show that lettuce inoculation with the selected AMF can improve the growth and the nutritional quality of lettuce even at sub-optimal P. Leaf content of chlorophylls, carotenoids, and phenols, known as important bioactive compounds for human health, was higher in mycorrhizal lettuce plants compared with non-mycorrhizal plants. The antioxidant capacity in AMF plants showed higher values compared with control plants grown at optimal P nutrition level. Moreover, leaf gas exchanges were higher in inoculated plants than in non-inoculated ones. Nitrogen, P, and magnesium leaf content was significantly higher in mycorrhizal plants compared with non-mycorrhizal plants grown with the same P level. These findings suggest that F. mosseae can stimulate plants growth, improving the nutritional quality of lettuce leaves even when grown with sub-optimal P concentration.
Efficient Utilization of Fruit Peels for the Bioproduction of D-Allulose and D-Mannitol
Authors: Li Jin , Chen Jiajun , Xu Wei , Zhang Wenli , Chen Yeming , Mu Wanmeng
Published: November 2022

Currently, the demand for low-calorie sweeteners has grown dramatically because consumers are more mindful of their health than they used to be. Therefore, bioproduction of low-calorie sweeteners from low-cost raw materials becomes a hot spot. In this study, a two-stage strategy was established to efficiently utilize D-fructose from fruit and vegetable wastes. Firstly, ketose 3-epimerase was used to produce D-allulose from D-fructose of pear peels. Secondly, the residual D-fructose was converted to D-mannitol by the engineered strain co-expression of D-mannitol 2-dehydrogenase and formate dehydrogenase. Approximately 29.4% D-fructose of pear peels was converted to D-allulose. Subsequently, under optimal conditions (35 °C, pH 6.5, 1 mM Mn2+, 2 g/L dry cells), almost all the residual D-fructose was transformed into D-mannitol with a 93.5% conversion rate. Eventually, from 1 kg fresh pear peel, it could produce 10.8 g of D-allulose and 24.6 g of D-mannitol. This bioprocess strategy provides a vital method to biosynthesize high-value functional sugars from low-cost biomass.
Screening of Yeasts Isolated from Baijiu Environments for Producing 3-Methylthio-1-propanol and Optimizing Production Conditions
Authors: Ma Jinghao , Cheng Liujie , Zhang Yujiao , Liu Yuchun , Sun Qi , Zhang Jie , Liu Xiaoyan , Fan Guangsen
Published: November 2022

3-Methylthio-1-propanol (3-Met) is widely used as a flavoring substance and an essential aroma ingredient in many foods. Producing 3-Met by microbial transformation is green and eco-friendly. In the present study, one strain, YHM-G, which produced a high level of 3-Met, was isolated from the Baijiu-producing environment. Strain YHM-G was identified as Hyphopichia burtonii according to its morphological properties, physiological and biochemical characteristics, and ribosomal large subunit 26S rRNA gene D1/D2 domain sequence analysis. The optimal conditions for 3-Met production by YHM-G were obtained by single factor design, Plackett–Burman design, steepest ascent path design and response surface methodology as follows: 42.7 g/L glucose, pH 6, 0.9 g/L yeast extract, 6 g/L L-methionine (L-Met), culture temperature 28 °C, shaking speed 210 rpm, loading volume 50 mL/250 mL, inoculum size 0.5% (v/v), culturing period 48 h and 2.5 g/L Tween-80. Under these optimal conditions, the 3-Met production by strain YHM-G was 3.16 g/L, a value 88.1% higher than that before optimization. Strain YHM-G can also produce a variety of flavor compounds that are important for many foods. This strain thus has the potential to increase the abundance of 3-Met in some fermented foods and enhance their aroma profiles.
Fruit Wine Obtained from Melon by-Products: Physico-Chemical and Sensory Analysis, and Characterization of Key Aromas by GC-MS
Authors: Salas-Millán José Ángel , Aznar Arantxa , Conesa Encarnación , Conesa-Bueno Andrés , Aguayo Encarna
Published: November 2022

About 20% of fresh fruits and vegetables are rejected for not meeting the superficial aesthetic standards (color, shape, and size). Part of the food production is not used in the human food chain. The transformation of these fresh products into novel re-valuable ones is a challenge for a sustainable food industry. This research studies an alcoholic fermentation fruit-based wine from two melon (Cucumis melo L.) cultivars: Jimbee® (smooth and yellow skin with orange flesh) and Okashi® (netted yellow-orange skin with pale green flesh). The melon juice (must) was fermented by Saccharomyces cerevisiae and enriched in sucrose and organic acids to achieve alcoholic fermentation, acidity, and flavors, obtaining a fruity-flavored and dry melon-based wine with 10° alcoholic grade, in both melon cultivars. The volatile compounds were measured by GC-MS and the odor activity value (OAV) was calculated. The Jimbee and Okashi melon wines increased their aromatic profile due to an increment in medium-chain fatty acid ethyl esters such as ethyl hexanoate, ethyl octanoate, and ethyl decanoate (OAV > 1), which contributed to the fruity aroma. Other volatile compounds such as ethyl 9-decenoate and phenethyl acetate (OAV > 1) appeared in the Okashi wine, which brought a floral aroma. For sensory evaluation (40–100), the Jimbee cultivar, with its orange flesh, scored 68.2 and the Okashi cultivar, with pale green flesh, scored 82.8, which was the preferred melon-based wine. This is an example of a circular economy model to produce a fruit-based wine with commercial potential and satisfactory sensory evaluation.
Nutritional Quality, Techno-Functional Characteristics, and Safety of Biomass Powder and Protein Isolate Produced from Penicillium maximae
Authors: Moura Marília A. F. , Alves Viviane S. , Takahashi Jacqueline A.
Published: November 2022

This study investigated the suitability of Penicillium maximae biomass powder and protein isolate as a food product or food ingredient. The biomass powder is rich in proteins (34.8%) and insoluble fiber (36.2%) but poor in lipids (3.1%). Strong water hydration (8.3 g/g, 8.5 g/g) and oil holding (6.9 g/g, 16.3 g/g) capacity were observed in the biomass powder and protein isolate, respectively, besides 100% emulsion stability, indicating multiple applications in the food industry. No locomotor impairment was induced in Drosophila melanogaster flies after consuming extracts of P. maximae biomass powder. Furthermore, decreased production of reactive oxygen species and preservation of survival, viability, and fertility parameters were observed in the nematode Caenorhabditis elegans, which reinforces the potential of P. maximae biomass for human and animal consumption. Together, the results show the vast food applicability of P. maximae biomass and protein isolate as protein substitutes with several health and environmental benefits.
Simultaneous Determination of 11 Mycotoxins in Maize via Multiple-Impurity Adsorption Combined with Liquid Chromatography–Tandem Mass Spectrometry
Authors: Guan Xin , Feng Yuchao , Suo Decheng , Xiao Zhiming , Wang Shi , Liang Ying , Fan Xia
Published: November 2022

In this study, multiple-impurity adsorption purification (MIA) technologies and liquid chromatography–tandem mass spectrometry (LC-MS/MS) were used to establish a method for detecting 11 mycotoxins in maize. The conditions for mass spectrometry and MIA were optimized. Maize was extracted with 70% acetonitrile solution, enriched, and purified using MIA technologies, and then, analyzed via LC-MS/MS. The results showed that the linear correlation coefficients of the 11 mycotoxins were >0.99, the sample recoveries ranged from 77.5% to 98.4%, and the relative standard deviations were <15%. The validated method was applied to investigate actual samples, and the results showed that the main contaminating toxins in maize were aflatoxins (AFs), deoxynivalenol (DON), fumonisins (FBs), ochratoxin A (OTA), and zearalenone (ZEN). Additionally, simultaneous contamination by multiple toxins was common. The maximum detection values of the mycotoxins were 77.65, 1280.18, 200,212.41, 9.67, and 526.37 μg/kg for AFs, DON, FBs, OTA, and ZEN, respectively. The method is simple in pre-treatment, convenient in operation, and suitable for the simultaneous determination of 11 types of mycotoxins in maize.
The Molecular Weight Distribution of Occluded Hydrocarbon Gases in the Khibiny Nepheline–Syenite Massif (Kola Peninsula, NW Russia) in Relation to the Problem of Their Origin
Authors: Nivin Valentin A. , Pukha Vyacheslav V. , Mokrushina Olga D. , Mikhailova Julia A.
Published: November 2022

The origin of hydrogen–hydrocarbon gases present in the rocks of the Khibiny massif in unusually high concentrations has been the subject of many years of discussion. To assess the role of potential mechanisms and relative time of formation of gases occluded in inclusions in minerals, the molecular weight distribution of C1–C5 alkanes in the main rock types of the Khibiny massif was studied. For this purpose, the occluded gases were extracted from rocks by mechanical grinding and their composition was analyzed on a gas chromatograph. It is established that the molecular weight distribution of occluded hydrocarbon gases in the Khibiny massif corresponds to the classical Anderson–Schulz–Flory distribution. In addition, the slopes of the linear relationships are relatively steep. This indicates a predominantly abiogenic origin of the occluded gases of the Khibiny massif. At the same time, a small proportion of biogenic hydrocarbons is present and is associated with the influence of meteoric waters. It was also found that in the Khibiny massif, the proportion of relatively high-temperature gases decreases towards the Main foidolite Ring in the following sequence: foyaite and khibinite–trachytoid khibinite–rischorrite and lyavochorrite–foidolites and apatite–nepheline ores. In the same sequence, an increase in the proportion of heavy hydrocarbons and the increasing role of oxidation and condensation reactions in the transformation of hydrocarbons occurs.
Frasassi Caves and Surroundings: A Special Vehicle for the Geoeducation and Dissemination of the Geological Heritage in Italy
Authors: Farabollini Piero , Bendia Fabrizio
Published: November 2022

One of the most fascinating aspects of the work of geologist lies in knowing how to read the physical landscape as an expression of the geological and geomorphological phenomena that shaped the Earth’s relief over time. The necessity to disseminate these vast areas of knowledges and skills starts from here, with the aim to enhance the concept of geodiversity and to raise awareness for its protection and promotion. This work aims to share some examples of projects realized following the subscription of agreement between different public authorities in the Apennine territory of Marche Region in Italy, such as the Geology Section of the University of Camerino (MC), “Consorzio Frasassi” (which manages the underground karst complex of Frasassi caves) and municipality of Genga (AN), where Frasassi caves are located. Thanks to this partnership, our research group realized didactic geological notebooks for school groups visiting the caves and interactive museum laboratories in 3D, showing the geological evolution of the area. This sharing of knowledge will contribute to educate communities about the importance of the geological heritage. At the same time, this project can serve as motivation to establishing the same type of collaborations in those territories where similar projects can be replicated.
Teaching Practical Skills in Anesthesia, Intensive Care, Emergency and Pain Medicine—What Is Really Relevant for Medical Students? Results of a German National Survey of Nearly 3000 Anesthesiologists
Authors: Busch Franziska , Weissenbacher Andreas , Stehr Sebastian N. , Piegeler Tobias , Hempel Gunther
Published: November 2022

As a part of a major reform of the medical curriculum in Germany, the national catalogue of learning objectives is being revised with the focus shifting from theory-based learning to teaching practical skills. Therefore, we conducted an online survey to answer the question, which practical skills are essential in anesthesia. Participants were asked to rate the relevance of several skills, that medical students should be able to perform at the time of graduation. A total of 2898 questionnaires could be evaluated. The highest ratings were made for “bringing a patient into lateral recumbent position” and “diagnosing a cardiac arrest”. All learning objectives regarding regional anesthesia were rated as irrelevant. Furthermore, learning objectives like “performing a bronchoscopy” or “performing a rapid sequence induction” had low ratings. In the subgroup analysis, physicians with advanced training and those who were working at university hospitals rated most skills with higher relevance compared to others. Our survey provides a good prioritization of practical skills for the development of new curricula and assessment frameworks. The results can also help to establish our discipline as a cross-sectional subject in competency-based medical education, thus further increasing the attractiveness for medical students.
Pulmonary Crohn’s Disease or Crohn’s Disease with Lung Sarcoidosis? A Case Report and Literature Review
Authors: Vlăsceanu Silviu , Bobocea Andrei , Petreanu Cornel Adrian , Bădărău Ioana Anca , Moldovan Horațiu , Gheorghiță Daniela , Antoniac Iulian-Vasile , Mirea Liliana , Diaconu Camelia Cristina , Savu Cornel
Published: November 2022

Background: Crohn’s disease and ulcerative hemorrhagic colitis are forms of granulomatous inflammatory intestinal disease, which usually affects the gastrointestinal tract. There are also reported rare localizations at the skin, kidney, joints, liver and eye level. Pulmonary involvement is relatively rare, and it is most commonly reported in suppuration with bronchiectasis. On the other hand, sarcoidosis is, in principle, a thoracic localization of a granulomatosis disease, although bowel, skin and intestinal disorders are described. There is not a clear line to separate Crohn’s disease from sarcoidosis with, possibly because they are, in fact, considered to have the same inflammatory granulomatosis disease pathology. The diagnoses of the two entities, sarcoidosis and Crohn’s disease, are based on non-pathognomonic, inclusive clinical and paraclinical criteria, without elements of the mutual exclusion of typical locations. Case Report: We present a very rare case of a young male, already diagnosed with small-bowel Crohn’s disease. Granulomatous lung disease with major hemoptysis requires emergency surgery. An intraoperative assessment revealed a necrotic hemorrhagic lesion located in the left lower lobe and a lobectomy was performed. The final pathological report showed the presence of non-caseous granulomatous inflammation, with the identification of specific multinucleated giant cells. Conclusions: The identical diagnostic principles of Crohn’s disease and sarcoidosis, Crohn’s disease as a predecessor to pulmonary lesions, the clinical picture and the necrotico-hemorrhagic appearance of the unilateral pulmonary lesion, which are similar to aggressive necrotico-hemorrhagic or perforating intestinal forms, are arguments in favor of the diagnosis of pulmonary Crohn’s disease and not pulmonary sarcoidosis. At the same time, in general, the two diseases have overlapping elements, suggesting they are, in fact, not the same disease with different facets.
Validation of the WHOQOL-BREF Quality of Life Questionnaire in an Urban Sample of Older Adults in a Neighbourhood in Zaragoza (Spain)
Authors: Gil-Lacruz Marta , Cañete-Lairla Miguel , Navarro Jorge , Montaño-Espinoza Rosa , Espinoza-Santander Iris , Osorio-Parraguez Paulina
Published: November 2022

Background: Nowadays, the increase in life expectancy needs to be matched by an increase in the wellbeing of older adults. A starting point is the definition of what is understood by health-related quality of life and its measurement in different contexts. Our research translates these international priorities to a local base. Objective: To evaluate the psychometric characteristics of the World Health Organization Quality of Life Questionnaire (WHOQOL-BREF) in a sample of older adults from a Spanish urban community (Casablanca). Methods: In collaboration with the local health centre, we designed and implemented the health neighbourhood survey. Interviews took place at subjects’ homes with 212 women and 135 men over the age of 60, who were residents in Casablanca. With the results, we evaluated the psychometric characteristics of WHOQOL-BREF and tested its reliability and validation. Results: The instrument has a high internal consistency with a Cronbach’s Alpha of 0.9. The items with higher correlation value were: ability to carry out activities in daily life, enough energy for daily life. The scale contributions of Physical Health dimension (0.809) and Psychological Health dimension (0.722) were notable. Conclusions: As with other studies, the instrument proved to be an integral evaluation of the diverse domains that condition the wellbeing of older adults.
Adapting and Validating the COVID-19 Vaccine Hesitancy and Vaccine Conspiracy Beliefs Scales in Korea
Authors: Ock Hyesung , Seong Mihyeon , Kim Insook
Published: November 2022

The coronavirus (COVID-19) pandemic has emphasized a need to assess the cause of vaccine hesitancy. This study verified the reliability and validity of the Korean versions of the COVID-19 vaccine hesitancy scale and vaccine conspiracy belief scale and the correlation between them. The COVID-19 vaccine hesitancy scale, Korean COVID-19 vaccine hesitancy scale, vaccine conspiracy beliefs scale, and self-efficacy scale were the study tools. Following translation into Korean, back translation into English, content validity verification, and preliminary survey, valid samples were obtained from 400 adults aged >20 years. Exploratory factor analysis revealed that “belief” and “lack of trust” accounted for 62.4% of the total variance. The model fit index of the vaccine conspiracy beliefs scale revealed that all values were in a good range. The Korean version of the COVID-19 vaccine hesitancy scale showed a positive correlation with vaccine conspiracy beliefs (r = 0.74, p < 0.001) and a significant negative correlation with self-efficacy (r = −0.17, p < 0.001). The validity and reliability of the COVID-19 vaccine hesitancy scale and vaccine conspiracy beliefs scale were verified. The Korean versions of the two scales can contribute to programs that measure and mediate various factors influencing vaccination during the COVID-19 pandemic.
Growth and Quality of Strawberry (Fragaria ananassa Dutch. cvs. ‘Kuemsil’) Affected by Nutrient Solution Supplying Control System Using Drainage Rate in Hydroponic Systems
Authors: Choi Su-Hyun , Kim Dae-Young , Lee Sun Yi , Chang Min-Sun
Published: November 2022

Although hydroponic techniques enable intensive and effective crop management, controlling the watering for each crop type and growth stage is challenging using the commonly used timed watering system. Therefore, excessive and insufficient watering occurs frequently. Hydroponic technology that considers the drainage rate can minimize the amount of drainage, thereby reducing environmental pollution and fertilizer consumption. This study compared the drainage rate and the timed watering methods in a strawberry hydroponic system and determined the optimal drainage rate. As the drainage rate increased, the amount of water supplied increased. A significantly negative correlation was found between the amount of water supplied and the total nitrogen content. Drainage electrical conductivity (EC) level was the highest in the 10% drainage rate group. In the 20% drainage rate treatment, leaf length and width increased compared to the four applications timer-supplying method. The yield of fruits weighing more than 27 g was the highest in the 20% drainage rate treatment. Therefore, the 20% drainage rate treatment was the most appropriate. We propose that it is possible to reduce unnecessary fertilizer consumption and increase productivity by controlling the water supply using the drainage rate for precise water supply management of strawberries.
Establishment of a Mutant Library of Fragaria nilgerrensis Schlechtendal ex J. Gay via EMS Mutagenesis
Authors: Jiang Shu , Wang Mingqian , Zhao Can , Cui Yuchen , Cai Zhi , Zhao Jun , Zheng Yang , Xue Li , Lei Jiajun
Published: November 2022

The diploid wild strawberry Fragaria nilgerrensis Schlechtendal ex J. Gay mainly distributed in Southwest China has many excellent traits and a small genome. A high-quality genome of F. nilgerrensis is available, but functional genomic research remains scarce. In the present study, to promote functional genomic research of F. nilgerrensis, ethyl methane sulfonate (EMS) was used to mutagenize the apical meristems, and the appropriate EMS mutagenesis dosages were screened. After treatment of 1200 apical meristems with 0.6% EMS for 6 h, a mutant library consisting of 86 mutant individuals, which were characterized by 17 mutant types, with a mutation rate of 7.17% was established. The characteristics of mutants included changes in the color, shape, number and size of leaves, and the architecture of flower and plant. The obtained mutants were identified by morphological appearance, botanical indexes, chlorophyll, photosynthetic fluorescence assays, root-tip chromosome, and flow cytometry observation. These mutants can provide great resources for gene functional research and future breeding of F. nilgerrensis.
Excessive Delay in Nutrient Release by Controlled-Release Fertilizers Can Reduce Chestnut Yield
Authors: Arrobas Margarida , Belotto Leonardo Bomfim , Marchetti Juliana Aparecida , Barroso Valdemar , Raimundo Soraia , Cassol Luís César , Correia Carlos Manuel , Rodrigues Manuel Ângelo
Published: November 2022

Farmers are increasing the use of fertilizers in chestnut, the only cash crop produced in the mountainous areas of northeastern Portugal. This calls for more studies to guide them towards a more ecological intensification. The effects of three controlled-release fertilizers, two that release nutrients over three months (BoskGrow 20:05:20_3m, Exactyon 18:05:13_3m) and one over six months (Exactyon 18:05:12_6m), and an organic amendment authorized for organic farming (Humix 12:03:05) were compared with an untreated control during a three-year field trial (2019–2021). BoskGrow 20:05:20_3m, Exactyon 18:05:13_3m and Humix 12:03:05 gave significantly higher nut yields (90.6 to 97.0 kg tree−1, average 2019 + 2021) than Exactyon 18:05:12_6m (66.3 kg tree−1) and the control (69.5 kg tree−1). Leaf concentrations of nitrogen and potassium tended to be higher in the BoskGrow 20:05:20_3m and Exactyon 18:05:13_3m treatments, and they were stated as the most important causes in the establishment of the two productive groups. Humix 12:03:05, although less concentrated in nutrients, led to a chestnut yield at the level of the most productive treatments, possibly due to the multiple positive effects of organic matter on soil and plants. Under the conditions of this experiment, where rainfall is low in the summer, fertilizers whose nutrient release takes a long time, such as Exactyon 18:05:12_6m, seem not to be a good fertilization option due to reduced nutrient uptake and increased levels of soil inorganic nitrogen at the end of the growing season. Humix 12:03:05 emerged as a possible solution for organic producers.
The MosHouse® Trap: Evaluation of the Efficiency in Trapping Sterile Aedes aegypti Males in Semi-Field Conditions
Authors: Kittayapong Pattamaporn , Kittayapong Rungrith , Ninphanomchai Suwannapa , Limohpasmanee Wanitch
Published: November 2022

Arbovirus diseases, such as dengue, chikungunya, and Zika, are important public health problems. Controlling the major vector, Aedes aegypti, is the only approach to suppressing these diseases. The surveillance of this mosquito species needs effective collecting methods. In this study, a simple MosHouse sticky trap was evaluated in a semi-field condition. Our results demonstrated the efficiency of this trap in collecting Ae. aegypti males, and no significant difference (p > 0.05) in the numbers of males was detected when compared with the widely used BG- Sentinel trap. However, there were significantly lower numbers of females (p < 0.05) collected using the MosHouse trap when compared to the BG-Sentinel trap. We also found a significant difference (p < 0.05) in the collected numbers between irradiated and non-irradiated males. More irradiated males were collected in the MosHouse traps. The improvement of male collection was achieved with the addition of a sugar stick and sticky flags. Significantly higher numbers of males were collected in the MosHouse trap with sticky flags compared to the original one when they were released independently of females, but both were collected in higher numbers when they were released together (p < 0.05). In conclusion, our experiments demonstrated that the MosHouse trap could sample Ae. aegypti, especially males, as efficiently as the established BG-Sentinel trap, while the cost was more than 50 times lower, showing the potential of the MosHouse trap for improved Ae. aegypti male and female surveillance with very large numbers of traps at affordable costs. In addition, significantly (p < 0.001) increased male sampling was achieved by adding an external sticky flag on the MosHouse trap, providing an avenue for further development of the novel male-trapping strategy.
Left Ventricular Diastolic Response to Isometric Handgrip Exercise in Physically Active and Sedentary Individuals
Authors: Rovithis Dimitrios , Anifanti Maria , Koutlianos Nikolaos , Teloudi Andriana , Kouidi Evangelia , Deligiannis Asterios
Published: November 2022

Aims: This study aims to investigate the diastolic left ventricular (LV) response to isometric handgrip exercise among healthy middle-aged men with high physical activity levels, versus matched sedentary individuals. Methods: Two groups of 10 men aged 41–51 years were studied. Men in the first group had high weekly self-reported physical activity levels (>3000 METs × min/week). In comparison, men in the second group reported low physical activity levels (<300 METs × min/week). An isometric handgrip exercise (IHE) stress echocardiography test was performed in all of them. Results: Both groups showed a similar and statistically significant increase in heart rate, systolic, diastolic, and mean arterial pressure following IHE. The group of active men under study did not show a statistically significant change in the ratio of early diastolic mitral valve inflow velocity to early diastolic lateral wall tissue velocity (E/e’ ratio) in response to IHE. Conversely, the inactive participants’ E/e’ ratio was higher at peak activity in the isometric handgrip exercise. Conclusions: Apparently, healthy middle-aged men with high levels of physical activity seem to have an improved lusitropic cardiac function compared to men with low levels of physical activity, as observed by the different diastolic LV responses induced by isometric handgrip exercise.
A 69 kb Deletion in chr19q13.42 including PRPF31 Gene in a Chinese Family Affected with Autosomal Dominant Retinitis Pigmentosa
Authors: Lan Yuanzheng , Chen Yuhong , Qiao Yunsheng , Xu Qingdan , Zhai Ruyi , Sun Xinghuai , Wu Jihong , Chen Xueli
Published: November 2022

We aimed to identify the genetic cause of autosomal dominant retinitis pigmentosa (adRP) and characterize the underlying molecular mechanisms of incomplete penetrance in a Chinese family affected with adRP. All enrolled family members underwent ophthalmic examinations. Whole-genome sequencing (WGS), multiplex ligation-dependent probe amplification (MLPA), linkage analysis and haplotype construction were performed in all participants. RNA-seq was performed to analyze the regulating mechanism of incomplete penetrance among affected patients, mutation carriers and healthy controls. In the studied family, 14 individuals carried a novel heterozygous large deletion of 69 kilobase (kb) in 19q13.42 encompassing exon 1 of the PRPF31 gene and five upstream genes: TFPT, OSCAR, NDUFA3, TARM1, and VSTM1. Three family members were sequenced and diagnosed as non-penetrant carriers (NPCs). RNA-seq showed significant differential expression of genes in deletion between mutation carriers and healthy control. The RP11 pedigree in this study was the largest pedigree compared to other reported RP11 pedigrees with large deletions. Early onset in all affected members in this pedigree was considered to be a special phenotype and was firstly reported in a RP11 family for the first time. Differential expression of PRPF31 between affected and unaffected subjects indicates a haploinsufficiency to cause the disease in the family. The other genes with significant differential expression might play a cooperative effect on the penetrance of RP11.
Promising Advances in Pharmacotherapy for Patients with Spinal Cord Injury—A Review of Studies Performed In Vivo with Modern Drugs
Authors: Mech Dominika , Korgol Katarzyna , Kurowska Antonina , Adamski Bartlomiej , Miazga Malgorzata , Biala Grazyna , Kruk-Slomka Marta
Published: November 2022

Spinal cord injury (SCI) is a pathological neurological condition that leads to significant motor dysfunction. It is a condition that occurs as a result of tragic accidents, violent acts, or as a consequence of chronic diseases or degenerative changes. The current treatments for patients with SCI have moderate efficacy. They improve the quality of life of patients, but they are still doomed to long-term disability. In response to the modern directions of research on possible therapeutic methods that allow for the recovery of patients with SCI, a scientific review publication is needed to summarize the recent developments in this topic. The following review is focused on the available pharmacological treatments for SCIs and the problems that patients face depending on the location of the injury. In the following review, the research team describes problems related to spasticity and neuropathic pain; possible therapeutic pathways are also described for neuroprotection and the improvement of neurotransmission within the injured spinal cord, and the review focuses on issues related to oxidative stress.
Prevalence of Venous Thromboembolism in Intensive Care Units: A Meta-Analysis
Authors: Gao Xiaoyu , Zeng Liangnan , Wang Haorun , Zeng Shan , Tian Junjie , Chen Ligang , Peng Tangming
Published: November 2022

Objective: Venous thromboembolism (VTE) is a life threating complication in intensive care units (ICUs). This study aimed to pool the prevalence of VTE and examined the risk factors of VTE in intensive care patients worldwide. Methods: A systematic search in PubMed, EMBASE and Web of Science databases was performed. Studies reported that the data on the prevalence of VTE or relevant information were synthesized using a random-effects model. Results: A total of 42 studies reporting on 27,344 patients were included. The pooled prevalence of VTE was 10.0% (95% CI: 7.0–14.0%). Subgroup and metaregression analyses found that thromboprophylaxis strategy, simplified acute physiology score (SAPS II), age, study quality, sample size, malignancy, sex, spinal cord injury and injury severity score (ISS) moderated the prevalence of VTE in intensive care patients. Conclusions: The present meta-analysis revealed a high prevalence of VTE in critically ill patients. The risk factors of VTE included thromboprophylaxis strategy, SAPS II, age, malignancy, sex, spinal cord injury and ISS. Therefore, we need to pay more attention to high-risk populations of VTE in intensive care patients.
Plasma Levels of CXC Motif Chemokine 1 (CXCL1) and Chemokine 8 (CXCL8) as Diagnostic Biomarkers in Luminal A and B Breast Cancer
Authors: Motyka Joanna , Gacuta Ewa , Kicman Aleksandra , Kulesza Monika , Ławicki Paweł , Ławicki Sławomir
Published: November 2022

Chemokines are involved in the regulation of immune balance and in triggering an immune response. CXCL1 and CXCL8 belong to the ELR-motif-containing group of CXC chemokines, which, in breast cancer (BC), stimulate angiogenesis and increase migration and invasiveness of tumor cells. The aim of this study was to evaluate CXCL1, CXCL8 and comparative marker CA 15-3 plasma concentrations in BC patients with luminal subtypes A and B. The study group consisted of 100 patients with BC, and the control group of 50 subjects with benign breast lesions and 50 healthy women. Chemokines concentrations were determined by ELISA method; CA15-3-by CMIA. Concentrations of CXCL8 and CA15-3 were significantly higher in BC total group and luminal B (for CA15-3 also in luminal A) subtype of BC than in healthy controls and subjects with benign lesions. In the total BC group, the highest SE, PPV and NPV were observed for CXCL8 (70%, 77.78%, 50%, resp.). A combined analysis of tested chemokines with CA 15-3 increased SE and NPV values (96%, 69.23%, resp.). The diagnostic power of the test (measured by area under ROC curve (AUC)) showed the highest value for CXCL8 in the total BC group (0.6410), luminal A (0.6120) and B subgroup of BC (0.6700). For the combined parameter, the AUC was increasing and reached the highest value for CXCL1 + CXCL8 + CA15-3 combination (0.7024). In light of these results, we suggest that CXCL8 could be used as an additional diagnostic marker that would positively influence the diagnostic utility of CA 15-3, especially in luminal B subtype of BC.
The Ocular Surface Symptoms and Tear Film Parameters during and after COVID-19 Infection
Authors: Szkodny Dominika , Wylęgała Adam , Chlasta-Twardzik Edyta , Wylęgała Edward
Published: November 2022

Purpose: This study aimed to evaluate the ocular surface parameters of post-COVID-19 patients when compared to healthy controls. Methods: Patients after symptomatic SARS-CoV-2 infection, as confirmed by a PCR test of their nasopharyngeal swab sample, were enrolled. Complete ophthalmic examination, including visual acuity test, intraocular pressure measurement (IOP), slit-lamp examination, tear osmolarity test, central corneal thickness, endothelial cell number measurements, non-invasive keratograph break-up time (NIKBUT), meniscus height, and the Schirmer’s test were performed and compared with the controls. Results: It must be noted that there were 36 COVID-19 patients and 25 control subjects included in the study. Eye itching and burning (19%) were the most common symptoms of COVID-19 infection, followed by a subjective decrease in vision (17%), conjunctivitis and eye pain were present in 11%, and 6% of the patients had episcleritis. The mean time from initial infection was 6.5 ± 3.9 (range 1–24 weeks). Meniscus height was not significantly changed between the COVID-19 (0.34 ± 0.13 mm) group and the control (0.33 ± 0.12 mm, p = 0.88) group. In addition, the NIKBUT-1 (p = 0.88; 7.22 ± 4.60 s and 6.91 ± 4.45 s) and NIKBUT average (p = 0.91, 12.30 ± 5.86 s and 11.77 ± 4.97 s) test results showed no significant change either. Neither was a significant result found in the IOP (p = 0.17, 14.56 ± 2.10 mmHg and 14.11 ± 1.96 mmHg); the Schirmer test (p = 0.18, 20.22 ± 7.92 mm and 20.02 ± 7.17 mm); Tosm (p = 0.16, 294.42 ± 54.51 mOsm/dL and 299.33 ± 5.65 mOsm/dL); CCT (p = 0.06, 549.15 ± 28.98 vs. 539.21 vs. 29.08 µm); nor the endothelial cell density (p = 0.07, 2516.64 ± 287.61 vs. 2454.21 ± 498.60 cells/mm2). Conclusions: Through this study it was not revealed that there were any significant differences between the post-COVID group and control group in the objective measurements of ocular surface conditions, when performed after the acute phase of COVID-19. The exact incidence and mechanism of ocular findings, especially dry eye disease, in correlation with SARS-CoV-2 requires further research.
Fecal Microbiota Transplantation May Represent a Good Approach for Patients with Focal Segmental Glomerulosclerosis: A Brief Report
Authors: Zhi Wenqiang , Yuan Xiaoli , Song Wenzhu , Jin Guorong , Li Yafeng
Published: November 2022

This is the first report of fecal microbiota transplantation (FMT) in patients with chronic kidney disease. The patient was subjected to focal segmental glomerulosclerosis (FSGS), with onset in April 2021. The main manifestation featured abnormal renal function and no proteinuria at the level of nephrotic syndrome. In May 2021, she showed biopsy-proven FSGS and was treated with glucocorticoid. However, after glucocorticoid reduction, the patient’s serum creatinine increased again, so she adjusted the dosage and continued use until now. In April 2022, the patient was prescribed the FMT capsules. After FMT, the renal function remained stable, urinary protein decreased, reaching the clinical standard of complete remission, and there was no recurrence after glucocorticoid reduction. Furthermore, the patient showed significantly decreased hyperlipidemia, triglyceride (TG) and cholesterol (CHO) after FMT. During FMT, the level of cytokines fluctuated slightly, but returned to the pre-transplantation level after three months. From this, we conclude that FMT is a potential adjuvant therapy for FSGS, and patients can benefit from improving renal function and dyslipidemia.
Bone Loss in Patients with Pancreatic Neuroendocrine Tumors
Authors: Tong He , Wang Miaomiao , Liu Jingjing , Guo Chuangen , Wang Zhongqiu , Wang Jianhua , Chen Xiao
Published: November 2022

Background: Pancreatic diseases may affect nutritional status, which is one of the important associated factors of bone health. High prevalence of osteoporosis or osteopenia has been reported in patients with pancreatitis. The bone loss in pancreatic neuroendocrine tumors (PNETs) has not been reported. In this study, we showed the prevalence of bone loss and possible associated factors in PNET patients. Methods: A total of 91 PNET patients were included. Bone status was evaluated based on computed tomography (CT) attenuation (Housfield units, HU): >160 HU, normal bone mineral density; osteopenia, 135 HU ≤ CT value ≤ 160 HU; osteoporosis, <135 HU. Associated factors for bone loss were identified by logistic regression analyses. Results: The average age was 55.76 years old in PNET patients. The prevalence of osteoporosis and low bone mass was 37.4% and 60.4%, respectively. Higher prevalence of osteoporosis was observed in patients older than 50 years (64.0%). Multivariate logistic analysis showed that age was an associated factor for low bone mass (odds ratio (OR) = 1.13, 95% confidence interval (CI): 1.04–1.22) and osteoporosis (OR = 1.14, 95% CI: 1.03–1.20). Diabetes was also associated with bone loss in PNET patients after adjusting with confounders (OR = 13.56, 95% CI: 1.02–132.4). Conclusions: Our data show that bone loss is common in patients with PNETs. Age and diabetes are associated with bone loss in PNET patients.
Graft Intra-Articular Remodeling and Bone Incorporation in ACL Reconstruction: The State of the Art and Clinical Implications
Authors: Moretti Lorenzo , Bizzoca Davide , Cassano Giuseppe Danilo , Caringella Nuccio , Delmedico Michelangelo , Moretti Biagio
Published: November 2022

The knee is one of the most frequently affected joints in sports trauma, and anterior cruciate ligament (ACL) injury and meniscal tears are the most common lesions. ACL reconstruction (ACLR) remains the treatment of choice for patients willing to return to their previous activity. There are different surgical techniques and different possible usable grafts. The graft used for ACLR surgery undergoes a bone incorporation process and an intra-articular remodelling named ligamentization until it reaches characteristics similar to the native ligament. After the first incorporation stage, the remodelling process is divided into an early stage that could last 4 weeks, a proliferative stage that lasts 4 to 12 weeks, and a final stage of ligamentization that could last over 1 year. The period of return to sport (RTS) after ACLR, which is becoming shorter and shorter, can be a high-risk period for athletes due to the risk of graft failure. This systematic review aims to define the phases of the ligamentization process considering graft type and fixation techniques, as well as the graft’s anatomopathological and biomechanical characteristics, to evaluate a criterion-based rehab progression and maximize patient outcomes for an RTS respecting graft biology. The rehabilitative program has to promote and optimize the graft remodelling and incorporation processes; moreover, it has to accommodate physiological graft healing and avoid overloading. An early RTS and noncompliance with the biological characteristics of the graft in the various phases are associated with a high incidence of re-injury.
Functional Outcome of All-Soft-Tissue Quadriceps Tendon Autograft in ACL Reconstruction in Young and Athletic Patients at a Minimum Follow-Up of 1 Year
Authors: Pichler Lorenz , Pichler Ludwig , Liu Markus , Payr Stephan , Binder Harald , Kaiser Georg , Hofbauer Marcus , Tiefenboeck Thomas
Published: November 2022

To investigate the functional outcome in young and athletic patients with ACL injuries, treated with an all-soft-tissue quadriceps tendon autograft at a minimum follow-up of 12 months. Methods: Patients who received a QT autograft ACL reconstruction between August 2018 and December 2020 were included in this study. Range of motion in the operated knee was described at 6 and 18 weeks after surgery and the functional outcome parameters (Lysholm score, IKDC score and Tegner activity scale) were calculated at 6 and ≥ 12 months after surgery. Results: Forty patients were included in this study, of which 29 identified as male and 11 as female. The average age was 31.3 years (range 16 to 57 years) and the mean follow-up time was 16.8 months (range 12 to 30 months). All functional outcome scores showed improvement over the course of the follow-up: Lysholm score 94.2 to 95.5 (n.s.), IKDC score 90.1 to 93.9 (n.s.), Tegner activity scale 3.7 to 5.0 (p > 0.001), all at six months and ≥12 months. No reruptures happened during the time of the follow-up. Conclusions: This study shows that the all-soft-tissue quadriceps tendon autograft technique can improve functional outcome in young and athletic patients with an ACL injury at short to intermediate follow-up.
Differences in Symptoms among Black and White Patients with ME/CFS
Authors: Jason Leonard A. , Torres Chelsea
Published: November 2022

Study samples of patients with myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) have primarily involved White subjects, so the literature on ethnic differences is sparse. The current study identified a sample of 19 Black patients diagnosed with ME/CFS and compared them with White patients with ME/CFS, as well as with healthy controls. The studies used a similar psychometrically sound assessment tool to assess symptoms in all subjects. Findings indicated there were significant differences between patients with ME/CFS versus controls, but few differences between patients who identified as Black or White. The results suggest there might be few symptom differences between patients with ME/CFS in these two ethnic groups. The implications of these findings are discussed.