bluuebunny/crossref_metadata_2025 huggingface dataset:
Synthesis and application of nanometer hydroxyapatite in biomedicine
Authors: Gui Xingyu , Peng Wei , Xu Xiujuan , Su Zixuan , Liu Gang , Zhou Zhigang , Liu Ming , Li Zhao , Song Geyang , Zhou Changchun , Kong Qingquan
Published: June 2022

Nano-hydroxyapatite (nano-HA) has been widely studied as a promising biomaterial because of its potential mechanical and biological properties. In this article, different synthesis methods for nano-HA were summarized. Key factors for the synthesis of nano-HA, including reactant concentration, effects of temperature, PH, additives, aging time, and sintering, were separately investigated. The biological performances of the nano-HA depend strongly on its structures, morphology, and crystallite sizes. Nano-HA with different morphologies may cause different biological effects, such as protein adsorption, cell viability and proliferation, angiogenesis, and vascularization. Recent research progress with respect to the biological functions of the nano-HA in some specific biological applications are summarized and the future development of nano-sized hydroxyapatite is prospected.
Insights From the NANOforArt Project: Application of Calcium-Based Nanoparticle Dispersions for Improved Preservation of Parchment Documents
Authors: Bartoletti Angelica , Odlyha Marianne , Bozec Laurent
Published: June 2022

Following pilot testing of the effects of alkaline-based nanoparticles on modern parchment, the treatment was extended to historical parchment. This paper describes the application and impact of calcium hydroxide and calcium carbonate nanoparticles dispersed in (i) propan-2-ol and (ii) cyclohexane on a parchment book cover dated 1570. The cover showed signs of damage resulting from contact with iron gall inks and low pH values (∼4–5). Protocols for the damage assessment of collagen in parchment as developed in the IDAP project (Improved Damage Assessment of Parchment) were used to evaluate the impact of the conservation treatments on parchment. Preliminary results have shown that the application of calcium-based nanoparticles did not produce any adverse effects on the state of preservation of collagen. In addition, positive outcomes emerged: the pH was re-adjusted to a neutral value, a strengthening and consolidation effect was observed, and the nanoparticles exhibited a protective action upon artificial ageing of the treated parchment.
Summerised Pharmacological Views on Dexmedetomidine
Authors: Abdul Kadar Nizamuddeen Kunai , AL Mustafa Mamdouh
Published: June 2022

Dexmedetomidine is selective alpha two adrenergic agonist like clonidine, but it is eight times more selective alpha two agonist than clonidine. Dexmedetomidine produces sedation and anxiolysis effect and it has analgesic effect like opioids but no respiratory depression, which makes it best for procedural sedation. Dexmedetomidine can be used for awake fiberoptic intubation, as it does not cause respiratory depression [1]. Atipamezole is a synthetic alpha two adrenergic receptor antagonist, which is used to treat over dose of dexmedetomidine. Dexmedetomidine is sold under the trade name called precedex, dexdomitor and dexdor. Case Study on dexmedetomidine is going on as it is new medicine which came in the market after 1999.
Preview of Single Nucleotide Polymorphism (Snp) and Potential Correlation between Human Genomes and Evolutionary History As Well As Religious Behaviors
Authors: Tang Bing
Published: June 2022

The story of a race to unlock the Holy Grail of biology is a fascinating and an exciting one. It appears to be an impossible task to predict the final outcome of the quest while there are so much left to play for, but undoubtedly, the swift advances in decoding the human genome and the tools available to decipher the genetic information will likely forever alter the practice of medicine. It will definitely affect anyone on this planet that have access to modern health care and will subsequently improve the condition of the entire human race.
Genotoxicity and Cytotoxicity Evaluation Applied to Environmental Health, Research of Polycyclic Aromatic Hydrocarbons
Authors: Frohlich Ane , Jaqueline Moura Dinara , Coelho Andre Leiliane
Published: June 2022

Environmental toxicology contributes for the evaluation of chemical exposures, proposing interventions based in preventive actions promoting a risk decrease. The industrial development, agriculture activities, the increase of the number of automobiles, among others, lead to a constant increase in the concentrations of air pollutants that are hazardous to health, therefore becoming a public health problem. Besides many chronic and acute respiratory diseases, related to environmental chemical exposures, the consequences related to genotoxic effects are poorly evidenced. Biomarkers of genotoxicity such as the comet assay, micronucleus assay, Salmonella/microsome assay and chromosomal aberration assay are reliable techniques for the early detection of the damage and for the evaluation of the chemical exposure aiming the prevention, mainly in environmental exposures, in which there is a really short window between the exposure to the chemical and the appearance of the damage. This paper aims to identify potential biomarkers of genotoxicity as reference to environmental chemical exposure, its usage and contributions to the decrease of this public health problem, especially for the Polycyclic Aromatic Hydrocarbons (PAHs). Keywords: biomarkers of genotoxicity; environmental health; PAHs;
Land Protection Partnership in the Montezuma Wetlands Complex, New York, USA
Authors: Wagner Matthew R. , Schummer Michael L. , Cripe Geoff , Halliday Mathew , Coluccy John M. , Fleming K. Sarah
Published: June 2022

Conserving critical wildlife habitat at a regional scale can be challenging, especially when the region hosts a range of land uses, jurisdictions, and competing interests. Abundant opportunities exist for cooperation when vested conservation entities find common ground to use their unique strengths in a cooperative effort to protect and restore wetlands for wildlife and people. We present the Montezuma Wetlands Complex (MWC) Land Protection Partnership as a case study of regional conservation collaboration aimed at identifying areas in greatest need of wetland protection and restoration to support wetland wildlife and provide wildlife-based recreation. The MWC is among the most important wetland complexes in the Atlantic flyway of eastern North America for migratory birds because it provides critical migratory stopover habitat for millions of birds and regionally unique habitats for breeding birds and resident wildlife, including numerous endangered and threatened (E&T) species. This case study demonstrates how state, federal, and nonprofit entities with differing goals and objectives can partner to protect and restore critical wetland habitat for wildlife. Partners optimized efforts by developing an online survey that included physical, land cover, biological, and people/use attributes which were ranked by each partner to determine common priorities and applied these into a spatial mapping, decision-support tool. Within attribute categories, land protection (physical), emergent marshes (land use), E&T (biological), and recreational areas (people/use) were highest ranked by partners. The decision-support tool provided an objective method of ranking parcels of land for public outreach efforts by the partners to protect and restore wetland wildlife habitat.
Nucleome programming is required for the foundation of totipotency in mammalian germline development
Authors: Nagano Masahiro , Hu Bo , Yokobayashi Shihori , Yamamura Akitoshi , Umemura Fumiya , Coradin Mariel , Ohta Hiroshi , Yabuta Yukihiro , Ishikura Yukiko , Okamoto Ikuhiro , Ikeda Hiroki , Kawahira Naofumi , Nosaka Yoshiaki , Shimizu Sakura , Kojima Yoji , Mizuta Ken , Kasahara Tomoko , Imoto Yusuke , Meehan Killian , Stocsits Roman , Wutz Gordana , Hiraoka Yasuaki , Murakawa Yasuhiro , Yamamoto Takuya , Tachibana Kikue , Peters Jan‐Michel , Mirny Leonid A , Garcia Benjamin A , Majewski Jacek , Saitou Mitinori
Published: June 2022

Germ cells are unique in engendering totipotency, yet the mechanisms underlying this capacity remain elusive. Here, we perform comprehensive and in‐depth nucleome analysis of mouse germ‐cell development in vitro, encompassing pluripotent precursors, primordial germ cells (PGCs) before and after epigenetic reprogramming, and spermatogonia/spermatogonial stem cells (SSCs). Although epigenetic reprogramming, including genome‐wide DNA de‐methylation, creates broadly open chromatin with abundant enhancer‐like signatures, the augmented chromatin insulation safeguards transcriptional fidelity. These insulatory constraints are then erased en masse for spermatogonial development. Notably, despite distinguishing epigenetic programming, including global DNA re‐methylation, the PGCs‐to‐spermatogonia/SSCs development entails further euchromatization. This accompanies substantial erasure of lamina‐associated domains, generating spermatogonia/SSCs with a minimal peripheral attachment of chromatin except for pericentromeres—an architecture conserved in primates. Accordingly, faulty nucleome maturation, including persistent insulation and improper euchromatization, leads to impaired spermatogenic potential. Given that PGCs after epigenetic reprogramming serve as oogenic progenitors as well, our findings elucidate a principle for the nucleome programming that creates gametogenic progenitors in both sexes, defining a basis for nuclear totipotency.
The Expression of the Dimensions of Smartness in Cultural Management: The Foundations of the Research
Authors: Milkintas Rytis , Tamošiūnas Teodoras
Published: June 2022

The aim of the research is to substantiate the methodological foundations of empirical research by revealing the concept of the expression of dimensions of smartness in cultural management. The article formulates theoretical construct based on expression of smartness dimensions in cultural management. This construct is formulating by combining cultural management and smart governance concepts and closely linking cultural management with the implementation of cultural policy and seeing the specifics of smart cultural management. The expression of smartness dimensions at culture management model consist of six main dimensions: strategics, creative development, harmonization of interests in the cultural sector, empowered cultural sector parties, harmony of intellectual and technological capital, the culture of shared value creation. Methodology strategy is based on two philosophical approaches by combining induction and deduction which result is abduction approach. Qualitative analysis was performed of theoretical sources of foreign countries and Lithuania. Another part of qualitative research is based on the interviewing chosen respondents to get information about the expression of smartness dimensions at culture management model functioning. There would be chosen about 30 experts (till data saturation) by using quadruple helix. Experts are chosen from government, science, business and nongovernmental organizations’ sphere. Gained results let to form research results conception which consists of three parts: theoretical model, current situation model, perspective situation model. The trajectory of the change from the current situation model to the perspective situation model helps to highlight the areas requiring improvement of the functioning of the cultural field, planned directions (trends) of change.In future research, it is planned to form the indicators for all 6 theoretical dimensions of the expression of smartness dimensions at culture management model. Analysis based on this theoretical model also will be performed at the state level which provide a comprehensive view of the cultural field current situation and hypothetical perspective situation.
Medications and Adherence to Treatment Guidelines Among Children Hospitalized With Acute COVID-19
Authors: Burns Julianne E. , Thurm Cary , Antoon James W. , Grijalva Carlos G. , Hall Matt , Hersh Adam L. , Hester Gabrielle Z. , Korn Emilie , Reyes Mario A. , Shah Samir S. , Totapally Balagangadhar R. , Teufel Ronald J.
Published: June 2022

OBJECTIVES

Coronavirus disease 2019 (COVID-19) treatment guidelines rapidly evolved during the pandemic. The December 2020 Infectious Diseases Society of America (IDSA) guideline, endorsed by the Pediatric Infectious Diseases Society, recommended steroids for critical disease, and suggested steroids and remdesivir for severe disease. We evaluated how medications for children hospitalized with COVID-19 changed after guideline publication.

METHODS

We performed a multicenter, retrospective cohort study of children aged 30 days to <18 years hospitalized with acute COVID-19 at 42 tertiary care US children’s hospitals April 2020 to December 2021. We compared medication use before and after the December 2020 IDSA guideline (pre- and postguideline) stratified by COVID-19 disease severity (mild–moderate, severe, critical) with interrupted time series.

RESULTS

Among 18 364 patients who met selection criteria, 80.3% were discharged in the postguideline period. Remdesivir and steroid use increased postguideline relative to the preguideline period, although the trend slowed. Postguideline, among patients with severe disease, 75.4% received steroids and 55.2% remdesivir, and in those with critical disease, 82.4% received steroids and 41.4% remdesivir. Compared with preguideline, enoxaparin use increased overall but decreased among patients with critical disease. Postguideline, tocilizumab use increased and hydroxychloroquine, azithromycin, anakinra, and antibiotic use decreased. Antibiotic use remained high in severe (51.7%) and critical disease (81%).

CONCLUSIONS

Although utilization of COVID-19 medications changed after December 2020 IDSA guidelines, there was a decline in uptake and incomplete adherence for children with severe and critical disease. Efforts should enhance reliable delivery of guideline-directed therapies to children hospitalized with COVID-19 and assess their effectiveness.
Soberanía y defensa de la riqueza forestal en la frontera vasconavarra con Francia durante el siglo XVIII
Authors: Aragón-Ruano Álvaro
Published: June 2022

La presente investigación trata de analizar el proceso de consolidación de la autoridad y soberanía de la Monarquía Hispánica sobre las riquezas forestales vasconavarras y, por ende, sobre la frontera pirenaica occidental, a fin de garantizar la provisión de maderas de la Real Armada. Dicho proceso culminó a finales del siglo XVIII, cuando, por un lado, en el contexto de la guerra contra Gran Bretaña, la Monarquía se vio obligada a acudir de forma intensiva a las reservas forestales del Pirineo vasconavarro, y, por otro, a tener un mayor control sobre la frontera con Francia, inmersa en un complicado período revolucionario. Si. hasta entonces. los conflictos en torno a las facerías y comunidades de pasto habían sido resueltos de una forma más o menos amistosa por las comunidades de ambos lados, a partir de finales del siglo xviii los Estados se vieron obligados a intervenir y, a través de la cartografía, a definir perfectamente sus límites.
Improvement of SERS signal measured by portable Raman instrument using random sampling technique
Authors: Van Tien Nguyen , Trong Nghia Nguyen , Thi Ha Lien Nghiem , Duong Vu , Quang Hoa Do , Chi Dung Duong , Nguyen Nhue Phan , Minh Hue Nguyen
Published: June 2022

In recent years, portable Raman spectrometers and commercialized surface-enhanced Raman scattering (SERS) substrates have become increasingly popular. They have turned out to be great tools for both substance detection, identification, and analysis on-site. This work addresses the technique to collect proper Raman spectra using SERS substrates and portable Raman spectrometers. We propose a random sampling technique that gives representative and high-quality spectra with high intensity and good resolution. This technique was tested on a home-built portable Raman spectrometer and SERS substrates based on metal film over nano-sphere (MFON) structure. Experimental results showed that peaks of Raman spectrum collected using random sampling technique are significantly narrower than those of spectra measured in conventional one and prevent samples and SERS substrates from photoinduced degradation. Potentially, this method can promote quantitative SERS and chemical trace analysis using portable Raman spectrometers.
Effect of extraction solvents on quality of vietnamese tea (Camellia sinensis O.Kuntze) seed oil
Authors: Thao Phan Thi Phuong , Anh Pham Le Nguyet , Vu Hong Son
Published: June 2022

Tea (Camellia sinesis) seed oil (TSO) is one of high-quality vegetable oils, similar to olive oil with high portion of unsaturated fatty acids, especially essential linoleic acid and low content of saturated fat. Solvent extraction method was used to obtain not only oil but also natural compounds from raw material. This study provides data on the physicochemical properties of TSO extracted from Trung du variety, cultivated in Phu Tho, Vietnam.
Preparation and analgesic activity test of Capsicum frutescens oleoresin
Authors: Do Hoang Minh Vo , Xuan Le Tien , Vo Mong Tham , Huynh Bui Thong , Nguyen Dinh Tien Dung , Thi Thuy Anh Nguyen , Truong Cong Tri , Nguyen Dai Hai
Published: June 2022

Capsaicin is an alkaloid in capsaicinoids group known as a spicy flavour ingredient in chilies and some pungent foods. Recently, capsaicin has been used not only as an additive in food but also as a medicine like analgesic, anticancer, and antidiabetic drug due to its’ pharmacological properties. In this study, capsicum oleoresin was extracted via heating chili powder, Capsicum frutescens (L.), with ethanol 80% at 70 °C in 120 minutes, then the extract was solvent evaporated in low pressure. Capsaicin was determined by thin-layer chromatography (TLC) and qualified by high-performance liquid chromatography (HPLC) method. Finally, the analgesic activity of capsicum oleoresin was tested on mice. The results show that capsicum oleoresin extracted from 2 kilograms of capsicum powder was 368.84 grams with 2.84% capsaicin. The central analgesic and peripheral analgesic effects of capsicum oleoresin were 90 minutes and 10-15 minutes after treatment, respectively.
Fuzzy distance-based filter-wrapper incremental algorithms for attribute reduction when adding or deleting attribute set
Authors: Phuong Ho Thi , Giang Nguyen Long
Published: June 2022

Attribute reduction is a critical problem in the data preprocessing step with the aim of minimizing redundant attributes to improve the efficiency of data mining models. The fuzzy rough set theory is considered an effective tool to solve the attribute reduction problem directly on the original decision system, without data preprocessing. With the current digital transformation trend, decision systems are larger in size and updated. To solve the attribute reduction problem directly on change decision systems, a number of recent studies have proposed incremental algorithms to find reducts according to fuzzy rough set approach to reduce execution time. However, the proposed algorithms follow the traditional filter approach. Therefore, the obtained reduct is not optimal in both criteria: the number of attribute of the reducts and the accuracy of classification model. In this paper, we propose incremental algorithms that find reducts following filter-wrapper approach using fuzzy distance measure in the case of adding and deleting attribute set. The experimental results on the sample datasets show that the proposed algorithms significantly reduce the number of attributes in reduct and improve the classification accuracy compared to other algorithms using filter approach
Análise das concepções de futuros professores de Ciências Biológicas sobre conceitos da Teoria Evolutiva
Authors: Angeloni-Duarte Mariana , Rosa Marcelo D’Aquino , Santos-Alfaya João Vicente
Published: June 2022

Resumo A teoria evolutiva é considerada o eixo integrador da Biologia, e, sendo assim, os conceitos relacionados à Evolução são necessários para uma adequada compreensão de outros modelos explicativos. O presente trabalho buscou analisar as concepções acerca de temas relacionados à Evolução Biológica (EB), por meio da aplicação de questionários entre os professores em formação em um curso de Ciências Biológicas. Após análise dos dados, percebeu-se que, além de equívocos relacionados a conceitos, uma parcela significativa dos respondentes não se sente segura para se manifestar a respeito dos tópicos sobre EB apresentados. Defendendo a ideia de que as concepções de futuros docentes não constituem um fim em si, foi feita uma articulação com discussões sobre formação de professores.
Problematizing of homeschooling: a defense of the public school as a common democratic space
Authors: Traversini Clarice Salete , Lockmann Kamila
Published: June 2022

Abstract This paper problematizes home schooling by analyzing bills currently under review in the Chamber of Deputies, as well as reports by mothers on the intensification of homeschooling during the coronavirus crisis. We understand that the proposal of homeschooling is aligned with a conservative neoliberal governmentality with a focus on the processes of individualization, exclusion, and teacher de-professionalization, which have prevented the schooling process from being shared in the common, public, and democratic school setting. As a form of resistance to such a process, we have brought up the concept of common as proposed by Dardot and Laval, which has enabled us to think of school as a non-appropriable setting.
Disciplina, biopolítica y educación: Un balance en torno a los estudios foucaultianos en educación
Authors: Espinel-Bernal Oscar
Published: June 2022

Resumen El ingreso de la vida en la política y el posicionamiento del Estado nación como forma de ordenamiento político y social hicieron de la educación un escenario fundamental en la consolidación, perfeccionamiento y desarrollo de las emergentes mecánicas del poder. Asimismo, los aparatos educacionales operaron decididamente en la fijación de la idea del bien común como horizonte de normalización. Ante la creciente amplitud de estudios y apropiaciones de las nociones foucaultianas en este escenario, se hace urgente adelantar un rastreo documental en torno al uso de las nociones de disciplina y biopolítica a fin de examinar las relaciones entre la institución del Estado como aparato de gobierno de la vida en la modernidad y la expansión de la maquinaria educacional.
Conhecimento dos enfermeiros recém-formados sobre Precauções Específicas na pré-pandemia da COVID-19
Authors: Eichemberger Júlia Rigolon , Kawagoe Julia Yaeko , Andrade Lívia Guimarães , Mohallem Andrea Gomes Costa
Published: June 2022

RESUMO Objetivo Avaliar o conhecimento dos enfermeiros recém-formados sobre as medidas das Precauções Específicas (PE). Método Estudo transversal descritivo-exploratório, com abordagem quantitativa, realizado em um hospital privado de São Paulo, em fevereiro de 2019. Os enfermeiros foram abordados para participar da pesquisa enquanto aguardavam o início do processo seletivo para “Enfermeiro Junior”. Para avaliar o conhecimento, foi utilizado um instrumento validado, contendo 29 questões de múltipla escolha, divididas em cinco eixos temáticos, sendo definida como ponto de corte a média 7,0 (de zero a 10). Para análise dos dados, foi usado o pacote estatístico R Core, e o lme4 para os modelos mistos generalizados, sendo adotado nível de significância de 5%. Resultados Participaram do estudo 190 enfermeiros recém-formados com nota geral de 5,82, sendo a maior média (7,9) no eixo “Equipamento de Proteção Individual”. Nos demais eixos, as médias foram abaixo de 7,0, tendo a menor pontuação (4,35) no eixo “Cuidados com o Ambiente”. Conclusão e Implicações para a prática O estudo evidenciou déficit de conhecimento sobre as PE entre os enfermeiros recém-formados, com implicações para a segurança dos pacientes e dos profissionais de saúde, desde a prática assistencial na graduação (estágios) ao início da carreira profissional.
Innovative isolation of colorant from Butea monosperma for surface-modified silk dyeing
Authors: Adeel Shahid , Abbas Shafqat , Habib Noman , Ticha Manel Ben , Batool Fatima , Mia Rony
Published: June 2022

Recently, there has been a surge of interest in adopting natural dyes to avoid the environmental difficulties connected with synthetic coloring compounds. This research focuses on extracting natural dyes from Butea monosperma plants and on developing sustainable dyeing methods for silk fabric. Natural colorants were extracted using an aqueous and an acidic extraction procedure, and silk fabrics were dyed with the extracts using mordanting with a variety of metal mordants and bio-mordants. The treatment of fabric and extracts was carried out using microwave (MW) irradiation. It was found that the bio-mordants have shown higher color strength than metallic mordant. It was also observed that the acacia and pine nut hull at 80°C and before dyeing pomegranate extract at 80°C and after dyeing of silk fabric at 40°C using bio-mordants gave higher color strength than metallic mordants. For comparative analysis, aluminum (Al) salt at 40°C, iron (Fe) salt at 60°C and tannic acid at 80°C gave acceptable results. The colorfastness characteristics were also found good to excellent. The bio-mordants not only provide the higher color strength and fastness properties, but these extracts have also shown an environmental friendly approaches. Scanning electron microscopy image and Fourier transform infrared spectroscopy analyses revealed the difference between irradiated and unirradiated silk fabrics. Therefore, it is recommended that MW rays and bio-mordants should be used in the natural dyeing of silk fabric to produce colorfast shades of acceptable strength.
Atik Şikâyet Defterleri'nin Vakıf Araştırmalarındaki Yeri: 36 Numaralı Atik Şikâyet Defteri Örneği
Authors: TUNÇ Muhammet Nuri
Published: June 2022

Osmanlı Devleti’nin toplumsal, kültürel, hukukî ve iktisadî yapısında önemli bir yeri olan vakıf müessesesiyle ilgili vakfiye, hurûfât, hazine (atik), şahsiyet, ahkâm, tafsil gibi defterlerin yanı sıra arşivlerde yer alan çeşitli fon ve kodlarla tasnif edilmiş çok sayıda belge vakıf araştırmalarında temel başvuru kaynağı olmuştur. Vakıflarla ilgili bu defter ve belgelerin yanı sıra Divân-ı Hümâyûn’un ana defter serisinden olan Atik Şikâyet Defterleri’nden, vakıflarla ilgili şikâyetleri, vakıfların işleyişini, vakıf görevlilerinin karşılaştıkları problemleri, vakıfların gelirleri, toprak anlaşmazlıkları gibi çok sayıda eşsiz bilgiyi tespit edebilmek mümkün olmaktadır. Bu araştırmada, 36 Numaralı Atik Şikâyet Defteri esas alınarak bu defter serisinin vakıf araştırmalarındaki yeri tespit edilmeye çalışılmıştır.
Arbeit und Psyche – eine Systematik der Selbstverständlichkeit
Authors:   Psychosocial Health and Well-being, EHS, Siemens AG, München, Borg Anna , Franke Ralf ,   Environmental Protection, Health Management and Safety (EHS), Siemens AG, München, Birner Ulrich ,   Environmental Protection, Health Management and Safety (EHS), Siemens AG, München
Published: June 2022

Psychosoziale Gesundheit wird zunehmend als Voraussetzung für Leistungsfähigkeit und Innovationskraft der Beschäftigten anerkannt und so zum kritischen Erfolgsfaktor im Unternehmen. Voraussetzung dafür sind Offenheit gegenüber dem Thema sowie seine konsequente Einbindung in das Arbeits- und Gesundheitsschutzmanagement. Darauf aufbauend können Aspekte des psychischen Wohlbefindens selbstverständlich und systematisch in wesentliche unternehmerische Handlungsfelder integriert werden.
Почему важна демография
Authors: Дорлинг Дэнни , Гител-Бастен Стюарт
Published: June 2022

Демография — не судьба. Как больше двух веков назад говорил Джакомо Казанова, «судьбы не существует, мы сами определяем свои жизни». Сегодня мы определяем свои жизни и жизнь общества больше, чем когда бы то ни было прежде. Еще никогда в мировом масштабе у нас не было так мало детей в пересчете на одного взрослого: численность нашего населения близка к стабилизации, хотя мы и не знаем, когда и при каких показателях это произойдет и что будет потом. Эта стабилизация станет результатом миллиардов частных решений, принятых под влиянием множества событий и политических мер, и некоторые из них будут менее предсказуемы, чем другие. Сегодня по миру передвигается больше людей, чем когда-либо прежде, но мы слишком часто воспринимаем их как угрозу, а не знак возросшей свободы. И точно так же мы слишком часто сокрушаемся по поводу старения населения, вместо того чтобы признать его следствием достижений человечества и адаптироваться к нему. Важность демографии лучше всего понятна, когда мы видим, что у нас есть выбор, осознаем изменения и не ограничены своими установками. В нарративах о демографии слишком долго преобладали пессимизм и упрощенческие, равнодушные к человеческим жизням формы учета. Как показывает этот увлекательный и убедительный обзор, нам стоит обновить свое понимание демографии.
Anesthetic Management of Critical COVID-19 Infection: A Narrative Review of Concepts and Evidence-Based Clinical Practices
Authors: Kassam Jameel , Mathew Eapen
Published: June 2022

Anesthesiologists are on the frontline in the war against the global COVID-19 pandemic, providing airway, ventilatory, and hemodynamic support to acute patients suffering from severe and critical COVID-19 infection. This is despite facing enhanced risk for cross-infection from patient respiratory secretions while performing intubations at high volume.
Refractory hematuria post actinic cystitis. Role of potassium alumen as treatment - Case Report
Authors: João Ernesto Aldred Pinto Filho , Pedro Henrique Ejje Kailly , Ruston da Matta Louback Filho , Juan Miguel Renteria , Antonio Claudio , Ahouagi Cunha
Published: June 2022

Actinic cystitis hematuria is a frequent complication after pelvic radiotherapy, present in approximately 5% of patients exposed to this therapy. The management of these cases seems to be a challenge for today’s urologists. In this article, we present the case of a 73- year-old man diagnosed with actinic cystitis after pelvic radiotherapy and admitted to our service with a clinical picture of refractory macroscopic hematuria. During admission, the patient underwent multiple procedures and blood transfusions, without success in controlling hematuria. After a review of the literature, we chose to use Potassium Alum in the patient’s treatment. Under general anesthesia, we performed a cystoscopy with the evacuation of clots and subsequently infused a 1% solution of potassium alum into the bladder at a rate of 200 ml/h for 25 hours. Less than 24 hours after Alum infusion, there were no more clinical signs of hematuria, which remained until hospital discharge. After 6 months of treatment, the patient did not present another episode of hematuria.
Totally implantable venous access port infection with Listeria monocytogenes: 2 case reports at a tertiary center and literature review
Authors: Christian Haddad , Rindala Saliba , Racha Ibrahim , Jacques Choucair , Nader Saad
Published: June 2022

Listeria monocytogenes infection in humans is mostly asymptomatic in immunocompetent patients. It rarely can cause self-limiting febrile gastroenteritis, bacteremia, meningoencephalitis, and maternal-neonatal infections. The latter three manifestations are the most common, especially among immunocompromised patients. We present two cases of Listeria monocytogenes bacteremia in cancer patients, a 79-year-old man and a 70-year-old woman in whom the source of bacteremia was identified to be the implantable venous access port (TIVAP). In both cases, the TIVAP has been removed after “positive control cultures the following readmission to the hospital despite targeted therapy with ampicillin and gentamicin”. Removal of TIVAP was warranted to control the infection.
Prevalence of ovine fasciolosis and its associated risk factors: The case of in and around Bedele Town, Bunno Bedele Zone, Oromia Region, Ethiopia
Authors: Diriba Tigire Nuqus , Dereje Abera Wako , Jiregna Dugassa Kitessa
Published: June 2022

Background: Ethiopia is rich in sheep and goats, accounting for 63% of the income from financial production and 23% of the cost of food. However, this immense wealth potential has not been well exploited for the support of farmers and their contribution to the economy as a whole due to various factors such as diseases. Among these, fascioliasis is one of the most economically important parasitic diseases of farm animals, especially in sheep. Objectives: To determine the prevalence and risk factors associated with ovine fasciolosis in the study area. Methods: A cross-sectional study design was conducted to determine the prevalence and risk factors associated with ovine fasciolosis in four randomly selected peasant associations in and around Bedele town by using standard fecal sedimentation techniques. Results: Out of a total of 384 sheep faecal samples processed and examined, 85 (22.1%) were positive for fasciolosis. Risk factors such as origin, age, sex, and body condition were also assessed for the occurrence of the disease. Accordingly; Shebe (26.4%) had a higher sheep fasciolosis, followed by Yabella (22.5%), Dabena Daru (20.8%), and Bedele 02 (17.5%) with no significant differences in peasant associations (p > 0.05). The prevalence rate under different body condition scores was recorded as poor (38.6%) compared to medium (22.3%), followed by good (16.3%). Similarly, infection was found to be among female (23%) and male (21%) sex groups; but had a statistically significant association (p < 0.05). Conclusion and Recommendations: Current research suggests that fasciolosis affecting sheep was a common parasitic disease in the study area, so control strategies should be developed to reduce the spread of infection.
Protocol for Agilent 1100 HPLC User Instructions v1
Authors: Wozniak Katherine
Published: June 2022

This protocol details instructions for using the Agilent 1100 HPLC, located at the Los Alamos National Laboratory TA-35 Building 85 Room 113. All users must be trained by Tari Kern, Steffi Davison, or Katie Wozniak before using the system. If someone else plans to use the HPLC after you, ensure that they plan to use the instrument that day. If so, you can leave it on after use. If not, turn it off.
[RETRACTED] Microbial Genome Editing v1
Authors: not provided contact.microbialtec
Published: June 2022

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Early microbial genome editing mainly focused on random screening and simple rational screening, but the shortcomings of traditional methods that are time-consuming, labor-intensive, work-intensive, and non-directional mutagenesis results have gradually been exposed over time. With the development of modern molecular biology technology, there are many new methods of microbial breeding with more directional and positive mutation, which greatly promotes the accuracy and efficiency of microbial genome editing.
Oligo pool library prep_forLANL v1
Authors: Kuo Rita
Published: June 2022

Issues need to be addressed during the library prep: 1. Primer dimmer is sever due to the fact that using long primers and cell culture as PCR template. d 2. An extra G appears on your reverse primers, which will mess up index reads and caused failure. An extra G on your read 2 primer region. P7 adapter - i7 index - Read 2 primer region template binding 5' CAAGCAGAAGACGGCATACGAGAT- index - GTGACTGG AGTTCAGACG TGTGCTCTTC CGATCT---AAC AGA TAA AAC GAA AGG CCC 3' I assume that LANL workflow should look like this: 1. E Coli Transformation 2. Recover culture in SOC 3. Grow cell in agar plates 4. Scrap cells and mix in LB (at least 50 ml?) that contained 10 % glycerol and antibiotics 5. Take 10 ml for plasmid extraction 6. Run PCR 7. Illumina library prep The protocols were designed based on LANL experiments and only help to resolve issues on step 6 and step 7. I offer 3 different approaches and please use your own judgement to chose the one that works for you.
Protocol for Nanocellect Wolf Cell Sorter v1
Authors: C Velasquez Guzman Jeanette
Published: June 2022

This protocol describes the use of Nanocellect Wolf Cell sorter that is located inside the anaerobic chamber in room 223A. The WOLF uses a disposable microfluidic cartridge that is connected to the Sample and Sheath tubing. This cartridge is where readouts and sorting happens. The machine itself contains no tubing or fluids.
SARS-CoV-2 live virus neutralization assay v2
Authors: Lassauniere Ria
Published: June 2022

Virus neutralization assays provide a means to quantitate functional antibody responses that block virus infection. These assays are instrumental in defining vaccine and therapeutic antibody potency, immune evasion by viral variants, and post-infection immunity. This protocol describe a means by which to measure neutralizing antibody titers in a live virus microneutralization assay specific for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The SARS-CoV-2 microneutralization assay is based on the influenza virus microneutralization assay described by WHO. In this assay, SARS-CoV-2 clinical isolates are pre-incubated with serial diluted antibody and added to Vero E6 cells. Replicating virus is quantitated by enzyme-linked immunosorbent assay (ELISA) targeting the SARS-CoV-2 nucleocapsid protein and the 50% virus inhibition titer calculated. The assay has been systematically optimized at critical test parameters that include virus titration, assay linearity, number of cells, viral dose, incubation period post-inoculation, and normalization methods. This protocol has three parts: Part I: Determination of the 50% tissue culture infectious dose per milliliter (TCID50/mL). Part II: ELISA. Part III: Virus microneutralization assay.
[RETRACTED] Keto Complete Reviews [Weight Loss]: Shocking Scam Report Revealed Must Read Before Buy Australia! v1
Authors: not provided health
Published: June 2022

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health
[RETRACTED] Pure Calms CBD Gummies Reviews- Updated 2022 v1
Authors: not provided officialcalmscbd
Published: June 2022

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On the other hand, you’re not taking any addictive or lab-made ingredients here. Instead, Pure Calm CBD Gummies work naturally with your body to give you the boost you need to feel great. The sooner you start taking these,
Protocol for BD FACSAria III User Instructions v1
Authors: Wozniak Katherine
Published: June 2022

This protocol details instructions for using the BD FACSAria III Flow Cytometery and Sorter, located at the Los Alamos National Laboratory HRL Building Room 228. All users must be trained by a Claire Sanders, Ramesh Jha, or Taraka Dale before using the system. Trained users can reserve Aria time via the LANL Outlook Calendar. If you notice that someone is signed up after you, ensure that they plan to use the instrument that day. If so, you can leave it on after use. If not, turn it off.
Loops of Violence(s) Within Europe’s Governance of Migration in Libya, Italy, Greece, and Belgium
Authors: Orsini Giacomo , Rota Marina , Uzureau Océane , Behrendt Malte , Adeyinka Sarah , Lietaert Ine , Derluyn Ilse
Published: June 2022

Studies have reported alarmingly high rates of traumatic experiences for refugee populations. While nearly all refugees experienced trauma in their country of origin, a vast majority of those seeking protection abroad also face (extreme) violence during their journeys and once in the country of destination. By concentrating on the migratory experiences of about 300 unaccompanied minors that we approached in Libya, Italy, Greece, and Belgium, this article analyses how different forms of violence are inflicted on these young migrants while moving to Europe. By concentrating on personal accounts of (recurrent) interactions with the EU migration and border management tools, we reveal the structural violence within the day-to-day governance of migration. Often framed as unintended or accidental, the article discusses how violence is instead ubiquitous, as it is systematically inflicted on migrants—including unaccompanied minors—in the form of repeated series of violent events or “loops of violence.” Importantly, such manifestations of violence are perpetrated by key institutional and non-institutional actors in the “migration industry” who are (in)directly involved in managing migration both inside and outside of the EU. Conceptually, we rely on K. E. Dempsey’s political geography of the different typologies of violence within Europe’s governance of migration and asylum and use it to concentrate on key transitional phases/fractures in migratory trajectories—i.e., as unaccompanied young migrants (try to) cross international borders and legal boundaries.
Features of conducting ethical review of research on artificial intelligence systems on the basis of the research and practical clinical center for diagnostics and telemedicine technologies of the Moscow Health Care Department, Moscow, Russian Federation
Authors: Pchelintseva Olga I. , Omelyanskaya Olga  V.
Published: June 2022

Ethical issues occupy a special place when conducting clinical trials of medicines, where it is impossible to predict in advance and accurately the effectiveness and safety of a new drug on the human body. Currently, during clinical trial examination, attention is paid to patients quality of life, and issues of compliance with patients rights, as well as compliance with the rules of good clinical practice and current legislation. Thanks to technological development, the number medical studies and devices using, among other things, specialized medical technologies and software is increasing.
Automation, development, improvement, and structuring of the processes involved cause an increase in the use of technical devices that use not only programs but also systems in their work. Artificial intelligence system software have a special place in medical science development.
Artificial intelligence, which was the field of science fiction 5080 years ago, is now firmly embedded in our everyday life. By introducing artificial intelligences capabilities into medical software, using it as part of medical equipment, and developing medical devices with artificial intelligence systems, we get a product that requires careful study and further development, which includes a complex of works on conducting scientific research, and registration and maintenance of such systems and complexes. All work is regulated by legislation in the field of circulation of medical devices and requires a deep systematic and scientific approach, including involving ethics to monitor compliance with the rights and safety of study participants and their medical data.
The Ethics Committee is an independent body that monitors compliance with the rights and requirements of legislation and conducts ethical and scientific examination of research documentation. Ethical issues when planning any research involving a person or his/her data should be discussed and considered in detail. The ethics committees should be contacted not only at the stage of approving research materials, but also when planning the design and developing research documentation and materials for patients, as well as regularly at all stages of the study.
Evaluation of the initial manifestations of the toxic process in conditions of chronic action of low subtoxic doses of dioxins polluting the environment
Authors: Lavrenov Anton R. , Ordzhonikidze Kristina G , Roumak Vladimir S. , Kim Alexander I. , Umnova Natalia V.
Published: June 2022

AIM: To study the state of the genome by indicators of retrotransposon activity the gene encoding DNA methyltransferase 1 (DNMT1); DNA damage in animals from the natural population of the bank vole (Clethrionomys glareolus) living in the vicinity of a preserved landfill contaminated with low concentrations of dioxins (landfill of production and consumption waste "Salariyevo", Moscow).
MATERIAL AND METHODS: The activity of ERV-L, B1, and L1 retrotransposons and the transcription level of the DNMT1 gene were evaluated by real-time PCR. The stability of DNA in liver and bone marrow cells was characterized by the comet assay method. Afterward, the obtained characteristics of the state (stability, reactivity, and damage) of the genome in response to environmental stress factors were compared in groups of animals from the study and conditionally control samples.
RESULTS: The effects of a decrease in the activity of retrotransposons of classes B1 and L1, and an increase in DNMT1 gene expression level were revealed in voles from the natural population living under the long-term chronic exposure to low doses of dioxins decrease in the activity of retrotransposons of classes B1 and L1, and an increase in the expression level of the DNMT1 gene were revealed. An increased level of DNA damage (on average up to 56% of the DNA in the tail of the comet) was detected in hepatocytes, with the addition of winter environmental factors to the chronic effect of small subtoxic doses of dioxins.
CONCLUSION: Suppression of retrotransposon activity and increased expression of its epigenetic regulator (DNMT1) are regarded as adaptive responses to long-term chronic exposure to low doses of dioxins polluting the environment. The alteration in the reactivity and destabilization of the genome indicates the launch of the initial mechanisms of the toxic process formation. The created and tested methodological base for its study opens up prospects for establishing a threshold level, and as a result, substantiating indicators for screening assessment of local (territorial) risk to public health by biomonitoring.
Pengembangan Modul Bimbingan Kelompok dengan Teknik Role Playing untuk Meningkatkan Empati Siswa SMP Laboratorium Universitas Negeri Malang
Authors: Dewi Ratriani Santi , Simon Irene Maya , Fauzan Lutfi
Published: June 2022

Abstract: The rapid development of the era and technology makes individuals become individuals who are increasingly indifferent to other people and the environment around them, this is also called low empathy. The purpose of this development research is to produce a product in the form of a Role Playing module Efforts to Increase Empathy for Middle School Students. The model in this study is Research and Development. development research obtained student data with an empathy scale for students, an assessment instrument for material & media experts, as well as an assessment of prospective users. The final validation of the assessment of the material expert test by 2 BK (Guidance and Counseling) lecturers, the media by 2 TEP (Educational Technology) lecturers, and prospective module users, namely 1 counselor and 2 students indicate that the module that has been developed is feasible and accepted by Laboratory Junior High School students Malang State University.

Abstrak: Pesatnya perkembangan jaman dan teknologi membuat individu menjadi pribadi yang semakin acuh tau acuh dengan orang lain maupun lingkungan disekitarnya, hal tersebut disebut juga rendahnya empati. Tujuan dari penelitian pengembangan ini yaitu untuk menghasilkan sebuah produk berupa modul Role Playing Upaya Meningkatkan Empati Siswa SMP. Model dalam penelitian ini yaitu Research and Development. penelitian pengembangan memperoleh data siswa dengan skala empati untuk siswa, instrumen penilaian ahli materi & media, serta penilaian uji calon pengguna. Akhir validasi penilaian dari uji ahli materi oleh 2 dosen BK (Bimbingan dan Konseling), media oleh 2 dosen TEP (Teknologi Pendidikan), dan calon pengguna modul yaitu 1 Konselor dan 2 siswa menunjukan bahwa modul yang telah dikembangakan sudah layak dan diterima siswa SMP Laboratorium Universitas Negeri Malang.