bluuebunny/crossref_metadata_2025 huggingface dataset:
Fruit-classification model resilience under adversarial attack
Authors: Siddiqi Raheel
Published: December 2021

An accurate and robust fruit image classifier can have a variety of real-life and industrial applications including automated pricing, intelligent sorting, and information extraction. This paper demonstrates howadversarial trainingcan enhance the robustness of fruit image classifiers. In the past, research in deep-learning-based fruit image classification has focused solely on attaining the highest possible accuracy of the model used in the classification process. However, even the highest accuracy models are still susceptible toadversarial attackswhich pose serious problems for such systems in practice. As a robust fruit classifier can only be developed with the aid of a fruit image dataset consisting of fruit images photographed in realistic settings (rather than images taken in controlled laboratory settings), a new dataset of over three thousand fruit images belonging to seven fruit classes is presented. Each image is carefully selected so that its classification poses a significant challenge for the proposed classifiers. Three Convolutional Neural Network (CNN)-based classifiers are suggested: 1)IndusNet, 2)fine-tuned VGG16, and 3)fine-tuned MobileNet. Fine-tuned VGG16 produced the best test set accuracy of 94.82% compared to the 92.32% and the 94.28% produced by the other two models, respectively. Fine-tuned MobileNet has proved to be the most efficient model with a test time of 9 ms/step compared to the test times of 28 ms/step and 29 ms/step for the other two models. The empirical evidence presented demonstrates that adversarial training enables fruit image classifiers to resist attacks crafted through the Fast Gradient Sign Method (FGSM), while simultaneously improving classifiers’ robustness against other noise forms including ‘Gaussian’, ‘Salt and pepper’ and ‘Speckle’. For example, when the amplitude of the perturbations generated through the Fast Gradient Sign Method (FGSM) was kept at 0.1, adversarial training improved the fine-tuned VGG16’s performance on adversarial images by around 18% (i.e., from 76.6% to 94.82%), while simultaneously improving the classifier’s performance on fruit images corrupted with ‘salt and pepper’ noise by around 8% (i.e., from 69.82% to 77.85%). Other reported results also follow this pattern and demonstrate the effectiveness of adversarial training as a means of enhancing the robustness of fruit image classifiers.
Integration of Droplet Microfluidic Tools for Single-Cell Functional Metagenomics: An Engineering Head Start
Authors: Conchouso David , Al-Ma’abadi Amani , Behzad Hayedeh , Alarawi Mohammed , Hosokawa Masahito , Nishikawa Yohei , Takeyama Haruko , Mineta Katsuhiko , Gojobori Takashi
Published: December 2021

Droplet microfluidic techniques have shown promising outcome to study single cells at high throughput. However, their adoption in laboratories studying “-omics” sciences is still irrelevant due to the complex and multidisciplinary nature of the field. To facilitate their use, here we provide engineering details and organized protocols for integrating three droplet-based microfluidic technologies into the metagenomic pipeline to enable functional screening of bioproducts at high throughput. First, a device encapsulating single cells in droplets at a rate of ∼250 Hz is described considering droplet size and cell growth. Then, we expand on previously reported fluorescence-activated droplet sorting systems to integrate the use of 4 independent fluorescence-exciting lasers (i.e., 405, 488, 561, and 637 nm) in a single platform to make it compatible with different fluorescence-emitting biosensors. For this sorter, both hardware and software are provided and optimized for effortlessly sorting droplets at 60 Hz. Then, a passive droplet merger is also integrated into our pipeline to enable adding new reagents to already-made droplets at a rate of 200 Hz. Finally, we provide an optimized recipe for manufacturing these chips using silicon dry-etching tools. Because of the overall integration and the technical details presented here, our approach allows biologists to quickly use microfluidic technologies and achieve both single-cell resolution and high-throughput capability (>50,000 cells/day) for mining and bioprospecting metagenomic data
Thyroid and parathyroid ectopia in the mediastinum; a case report
Authors: Kakamad Fahmi H. , Salih Abdulwahid M. , Ahmed Shaho F. , Abdullah Hiwa O. , Kakamad Suhaib H. , Mustafa Shevan M. , Mohammed Shvan H.
Published: December 2021

ABSTRACT

Introduction:

There are a variety of diseases that may appear as a mediastinum tumor, occurrence of ectopic parathyroid gland (EPG) and ectopic thyroid tissue (ETT) together in the mediastinum is a very rare entity. This study aims to report a case of associated ectopic thyroid and parathyroid in mediastinum.

Case presentation:

A 67-year-old lady complained of cough for two months aggravated by lying down, relieved by sitting and standing. Chest X-ray showed enlargement of superior mediastinum. A round non-homogenous, well-defined mass was seen in the superior of mediastinum by computed tomography (CT) scan. The patient underwent thoracotomy and the mass was totally resected. Histological examination revealed ectopic thyroid and parathyroid tissues in the superior mediastinum.

Clinical discussion:

The major reasons for originating thyroid ectopia in distant sites could be unordinary migration, abnormal differentiation of endodermal cells, or excessive decent of thyroglossal duct remnants is thought to be the cause of the ETT in mediastinum. Patients with thyroid ectopia of mediastinum commonly present with cough, dyspnea, stridor and dysphonia.

Conclusion:

Finding of ETT and EPG in the mediastinum is a very rare entity, which is caused by the abnormal migration during embryogenesis of the thyroid. The management includes total excision of the mass.

Highlights
Micronutrient's deficiency in India: a systematic review and meta-analysis
Authors: Venkatesh U. , Sharma Akash , Ananthan Velmurugan A. , Subbiah Padmavathi , Durga R. ,   CSIR Summer Research training team
Published: December 2021

India is coming to grips with a stage of nutrition transition. According to the Food Safety and Standards Authority of India (FSSAI), preventable micronutrient deficiency is arising public health precedence in India. However, the foremost public health concern is the lack of national prevalence data. The present study was carried out to estimate the pooled age-wise prevalence of six preventable micronutrient deficiencies (vitamin A, vitamin B12, vitamin D, iron, iodine and folic acid) in India. A systematic review was carried out on PubMed and Global Index Medicus databases using the Boolean search strategy. Statistical analyses were done using R software, version 3.6. 2. PRISMA guidelines were strictly adhered to during the review. A preliminary literature search yielded 4302 articles; however, 270 original research articles were found eligible to be included in quantitative synthesis. The estimated overall prevalence was 17 % [95 % confidence interval (CI) 0⋅07, 0⋅26] for iodine deficiency, 37 % (95 % CI 0⋅27, 0⋅46) for folic acid deficiency, 54 % (95 % CI 0⋅49, 0⋅59) for iron deficiency, 53 % (95 % CI 0⋅41, 0⋅64) for vitamin B12deficiency, 19 % (95 % CI 0⋅09, 0⋅29) for vitamin A deficiency and 61 % (95 % CI 0⋅07, 0⋅26) for vitamin D with high heterogeneity. We classified the population into infants (0–5 years), adolescents (<18 years), adults (>18 years) and pregnant women. Iron deficiency was most prevalent (61 %) in pregnant women. The results of the present study reinforce the data on micronutrient deficiency in India and warrant the immediate need for further active public health interventions to address these deficiencies. The study is registered with PROSPERO (CRD42020205043).
The oldest known record of a ground sloth (Mammalia, Xenarthra, Folivora) from Hispaniola: evolutionary and paleobiogeographical implications
Authors: Viñola-Lopez Lazaro W. , Core Suárez Elson E. , Vélez-Juarbe Jorge , Almonte Milan Juan N. , Bloch Jonathan I.
Published: December 2021

Sloths were among the most diverse groups of land vertebrates that inhabited the Greater Antilles until their extinction in the middle-late Holocene following the arrival of humans to the islands. Although the fossil record of the group is well known from Quaternary deposits in Cuba, Hispaniola, and Puerto Rico, remains from older units are scarce, limiting our understanding of their evolution and biogeographic history. Here we report the oldest known fossil ground sloth from Hispaniola, represented by an unassociated partial tibia and scapula that are recognized as a single taxon from the late Miocene-early Pliocene of the Dominican Republic. The combination of characters observed on the tibia suggests a close relationship with Megalocnus, otherwise only known from the Pleistocene–Holocene of Cuba. These fossils fill a temporal gap between those previously known from the early Miocene of Cuba and those from Pleistocene–Holocene deposits in the region and provide additional support for a continuous presence of the group in the Greater Antilles since the Oligocene.
Economic evaluation guidelines in low- and middle-income countries: a systematic review
Authors: Daccache Caroline , Rizk Rana , Dahham Jalal , Evers Silvia M. A. A. , Hiligsmann Mickael , Karam Rita
Published: December 2021

Objectives

To systematically identify the latest versions of official economic evaluation guidelines (EEGs) in low- and middle-income countries (LMICs) and explore similarities and differences in their content.

Methods

We conducted a systematic search in MEDLINE (Ovid), PubMed, EconLit, Embase (Ovid), the Cochrane Library, and the gray literature. Using a predefined checklist, we extracted the key features of economic evaluation and the general characteristics of EEGs. We conducted a comparative analysis, including a summary of similarities and differences across EEGs.

Results

Thirteen EEGs were identified, three pertaining to lower-middle-income countries (Bhutan, Egypt, and Indonesia), nine to upper-middle-income countries (Brazil, China, Colombia, Cuba, Malaysia, Mexico, Russian Federation, South Africa, and Thailand), in addition to Mercosur, and none to low-income countries. The majority (n = 12) considered cost–utility analysis and health-related quality-of-life outcome. Half of the EEGs recommended the societal perspective, whereas the other half recommended the healthcare perspective. Equity considerations were required in ten EEGs. Most EEGs (n = 11) required the incremental cost-effectiveness ratio and recommended sensitivity analysis, as well as the presentation of a budget impact analysis (n = 10). Seven of the identified EEGs were mandatory for pharmacoeconomics submission. Methodological gaps, contradictions, and heterogeneity in terminologies used were identified within the guidelines.

Conclusion

As the importance of health technology assessment is increasing in LMICs, this systematic review could help researchers explore key aspects of existing EEGs in LMICs and explore differences among them. It could also support international organizations in guiding LMICs to develop their own EEGs and improve the methodological framework of existing ones.
Decadent Experience: Conservatism and Modernity
Authors: Murray Alex
Published: December 2021

At the core of literary decadence is a conflicted relationship with modernity. For some decadent writers, the onset of rapid social and technological change could usher in possibilities for living and loving in hitherto unimagined ways, yet for others of a more conservative hue, modernization was to be rejected, tradition embraced. This essay argues that experience can be used as a framework for articulating these very different forms of decadence. The essay begins with an exploration of aesthetic modernity as an attempt to articulate the shock of the new, whereby the experience (present) or sensation becomes the ground for the erosion of collective tradition (experience past). Decadent and aestheticist writers such as Walter Pater, Arthur Symons, and Oscar Wilde embraced these new experiences, rejecting the “fruits of experience” as a ground for knowledge. In contradistinction to this valorization of sensation, I examine the “conservative” decadent aesthetic of Lionel Johnson and Michael Field. These writers’ embrace of nostalgia and jingoistic nationalism, I argue, demands we expand our current critical frameworks to more fully encompass the politics of decadence.
Absorption and Mindfulness Reflect Distinct Patterns of Attentional Control and Self-Related Processing
Authors: Leppanen Marcus L. , Kim Kyungmi
Published: December 2021

Abstract. Absorption and mindfulness are personality traits associated with experiencing states of highly-focused attention. Despite this apparent commonality, these two traits have rarely been directly compared, and little is known about what differentiates absorbed from mindful states. The present study explored how individual differences in the cognitive processes of attentional control and self-related processing relate to absorption and mindfulness. Participants completed four self-report measures assessing absorption, mindfulness, style of attentional control over internal/external stimuli, and self-related processing. Absorption and mindfulness were negatively correlated in our sample. Absorption was predicted by a propensity for stimulus-driven attention to external stimuli and frequent engagement in self-reflection without gaining insight into those reflections. Mindfulness was predicted by a propensity for goal-driven attentional control over external stimuli and a tendency to engage in insightful self-reflective processing. Our findings can inform research efforts to further elucidate cognitive mechanisms underlying the relationships of absorption and mindfulness with mental health.
Impacts of the Scale of Representation of Heterogeneity on Simulated Salinity and Saltwater Circulation in Coastal Aquifers
Authors: Yu Xuan , Michael Holly A.
Published: December 2021

Numerical models of variable‐density groundwater flow and salt transport are a primary tool for predicting salinity distributions in coastal aquifers and estimating submarine groundwater discharge (SGD). Models are particularly useful to estimate the saline component of SGD, which can occur far offshore and is difficult to measure directly. Depending on the system and application, the level of geologic detail represented can range from homogeneous or layered to fully heterogeneous hydraulic conductivity fields. These features strongly affect model results, limiting understanding of subsurface salinity distributions and associated density‐driven saltwater circulation along coasts worldwide. In this study, the impact of the scale of representation of heterogeneity on salinity distributions and SGD was investigated using numerical simulations. Upscaling hydraulic conductivity can significantly modify salinity distributions and flow paths, resulting in unpredictable variations in simulated SGD, though the values for homogeneous fields with equivalent hydraulic conductivity show consistent trends. Simulated density distributions control both the rate and direction of subsurface saltwater circulation. The length of the mixing zone perimeter, a measure of salinity distribution complexity, is shown to correlate with both the rate of subsurface saltwater circulation and the amount of groundwater circulating in the reverse direction from homogeneous cases. Overall, the results demonstrate a strong dependence of salinity distributions and saltwater circulation on the scale and distribution of geologic heterogeneity represented in numerical models. This suggests that numerical models with simplified geologic structure may substantially underestimate saltwater circulation, and attempts to calibrate them using salinity distributions or SGD measurements may be problematic.
Lasers and Ultracold Atoms for a Changing Earth
Authors: Van Camp Michel , dos Santos F. , Murb�ck Michael , Petit G�rard , M�ller J�rgen
Published: December 2021

Applying new technology rooted in quantum mechanics and relativity to terrestrial and space geodesy will sharpen our understanding of how the planet responds to natural and human-induced changes.
Tibetan Dust Accumulation Linked to Ecological and Landscape Response to Global Climate Change
Authors: Huang Xianmei , Miao Xiaodong , Chang Qiufang , Zhong Jiemei , Mason Joseph A. , Hanson Paul R. , Ou Xianjiao , Xu Liubing , Lai Zhongping
Published: December 2021

The Tibetan Plateau (TP) is a hotspot of earth system research, and understanding its landscape and ecosystem evolution has been hampered by the lack of time‐constrained geological records. Geochronological data from 14 loess sites covering a large region in the Tibetan interior show that the TP loess, rather than accumulating during glacial periods, began aggrading at either 13.4 ± 0.4 or 9.9 ± 0.2 ka. An ecological threshold was crossed, when warmer and wetter conditions resulted in increased vegetation cover enabling dust trapping. This dust accumulation model is out of phase with that of the Chinese Loess Plateau (CLP) where high sedimentation rates occurred during the cold/dry glacial stages. The TP loess accumulation is in response to global climate change, at both orbital (glacial/interglacial) and millennial (e.g., Younger Dryas event) time scales, despite more complexity via ecological and landscape processes than the CLP loess.
Upper Mantle Viscosity Underneath Northern Marguerite Bay, Antarctic Peninsula Constrained by Bedrock Uplift and Ice Mass Variability
Authors: Samrat Nahidul Hoque , King Matt A. , Watson Christopher , Hay Andrea , Barletta Valentina R. , Bordoni Andrea
Published: December 2021

We constrain viscoelastic Earth rheology and recent ice‐mass change in the northern Marguerite Bay region of the Antarctic Peninsula. Global Positioning System (GPS) time series from Rothera and San Martin stations show bedrock uplift range of ∼−0.8–1.8 mm/year over 1999–2005 and 2016–2020 but ∼3.5–6.0 mm/year over ∼2005–2016. Digital elevation models reveal substantial surface lowering, but at a lower rate since ∼2009. Using these data, we show that an elastic‐only model cannot explain the non‐linear uplift of the GPS sites but that a layered viscoelastic model can. We show close agreement between GPS uplift changes and viscoelastic models with effective elastic lithosphere thickness and upper‐mantle viscosity ∼10–95 km and ∼0.1−9 × 1018 Pa s, respectively. Our viscosity estimate is consistent with a north‐south gradient in viscosity suggested by previous studies focused on specific regions within the Antarctic Peninsula and adds further evidence of the low viscosity upper mantle in the northern Antarctic Peninsula.
Gut microbiota, innate immune pathways, and inflammatory control mechanisms in patients with major depressive disorder
Authors: Caso Javier R. , MacDowell Karina S. , González-Pinto Ana , García Saínza , de Diego-Adeliño Javier , Carceller-Sindreu Mar , Sarramea Fernando , Caballero-Villarraso Javier , Gracia-García Patricia , De la Cámara Concepción , Agüera Luis , Gómez-Lus María L. , Alba Claudio , Rodríguez Juan M. , Leza Juan C.
Published: December 2021

Although alterations in the gut microbiota have been linked to the pathophysiology of major depressive disorder (MDD), including through effects on the immune response, our understanding is deficient about the straight connection patterns among microbiota and MDD in patients. Male and female MDD patients were recruited: 46 patients with a current active MDD (a-MDD) and 22 in remission or with only mild symptoms (r-MDD). Forty-five healthy controls (HC) were also recruited. Psychopathological states were assessed, and fecal and blood samples were collected. Results indicated that the inducible nitric oxide synthase expression was higher in MDD patients compared with HC and the oxidative stress levels were greater in the a-MDD group. Furthermore, the lipopolysaccharide (an indirect marker of bacterial translocation) was higher in a-MDD patients compared with the other groups. Fecal samples did not cluster according to the presence or the absence of MDD. There were bacterial genera whose relative abundance was altered in MDD:Bilophila(2-fold) andAlistipes(1.5-fold) were higher, whileAnaerostipes(1.5-fold) andDialister(15-fold) were lower in MDD patients compared with HC. Patients with a-MDD presented higher relative abundance ofAlistipesandAnaerostipes(1.5-fold) and a complete depletion ofDialistercompared with HC. Patients with r-MDD presented higher abundance ofBilophila(2.5-fold) compared with HC. Thus, the abundance of bacterial genera and some immune pathways, both with potential implications in the pathophysiology of depression, appear to be altered in MDD, with the most noticeable changes occurring in patients with the worse clinical condition, the a-MDD group.
Genome-wide association and functional interrogation identified a variant at 3p26.1 modulating ovarian cancer survival among Chinese women
Authors: Dai Hongji , Chu Xinlei , Liang Qian , Wang Mengyun , Li Lian , Zhou Yao , Zheng Zhanye , Wang Wei , Wang Zhao , Li Haixin , Wang Jianhua , Zheng Hong , Zhao Yanrui , Liu Luyang , Yao Hongcheng , Luo Menghan , Wang Qiong , Kang Shan , Li Yan , Wang Ke , Song Fengju , Zhang Ruoxin , Wu Xiaohua , Cheng Xi , Zhang Wei , Wei Qingyi , Li Mulin Jun , Chen Kexin
Published: December 2021

Ovarian cancer survival varies considerably among patients, to which germline variation may also contribute in addition to mutational signatures. To identify genetic markers modulating ovarian cancer outcome, we performed a genome-wide association study in 2130 Chinese ovarian cancer patients and found a hitherto unrecognized locus at 3p26.1 to be associated with the overall survival (Pcombined = 8.90 × 10−10). Subsequent statistical fine-mapping, functional annotation, and eQTL mapping prioritized a likely casual SNP rs9311399 in the non-coding regulatory region. Mechanistically, rs9311399 altered its enhancer activity through an allele-specific transcription factor binding and a long-range interaction with the promoter of a lncRNABHLHE40-AS1. Deletion of the rs9311399-associated enhancer resulted in expression changes in several oncogenic signaling pathway genes and a decrease in tumor growth. Thus, we have identified a novel genetic locus that is associated with ovarian cancer survival possibly through a long-range gene regulation of oncogenic pathways.
Lipid droplet availability affects neural stem/progenitor cell metabolism and proliferation
Authors: Ramosaj Mergim , Madsen Sofia , Maillard Vanille , Scandella Valentina , Sudria-Lopez Daniel , Yuizumi Naoya , Telley Ludovic , Knobloch Marlen
Published: December 2021

Neural stem/progenitor cells (NSPCs) generate new neurons throughout adulthood. However, the underlying regulatory processes are still not fully understood. Lipid metabolism plays an important role in regulating NSPC activity: build-up of lipids is crucial for NSPC proliferation, whereas break-down of lipids has been shown to regulate NSPC quiescence. Despite their central role for cellular lipid metabolism, the role of lipid droplets (LDs), the lipid storing organelles, in NSPCs remains underexplored. Here we show that LDs are highly abundant in adult mouse NSPCs, and that LD accumulation is significantly altered upon fate changes such as quiescence and differentiation. NSPC proliferation is influenced by the number of LDs, inhibition of LD build-up, breakdown or usage, and the asymmetric inheritance of LDs during mitosis. Furthermore, high LD-containing NSPCs have increased metabolic activity and capacity, but do not suffer from increased oxidative damage. Together, these data indicate an instructive role for LDs in driving NSPC behaviour.
Survival benefit of platinum-based regimen in early stage triple negative breast cancer: A meta-analysis of randomized controlled trials
Authors: Bian Lei , Yu Ping , Wen Jiahuai , Li Na , Huang Wanwei , Xie Xiaoming , Ye Feng
Published: December 2021

Platinum (Pt)-based chemo-regimens have been proved effective in neoadjuvant and salvage chemotherapy of triple negative breast cancer (TNBC). However, the survival benefit of Pt-based regimens in early stage TNBC(eTNBC) treatment has remained unclear. We conducted a meta-analysis to explore its role in improving the clinical outcomes of eTNBC. We carried out a comprehensive literature search on 15 March 2021 for randomized controlled trials (RCTs) comparing ajuvant/neoadjuvant Pt-based and Pt-free chemo-regimens in eTNBC patients, according to PRISMA 2020. We extracted the survival data and utilized the STATA software to calculate the summarized hazard ratios (HRs) and 95% confidence interval (95% CI) for overall survival (OS) and disease-free survival (DFS). Seven eligible RCTs enrolling a total of 2,027 eTNBC patients were identified in this meta-analysis, with 1,007 receiving Pt-free regimens, and the other 1,020 patients receiving Pt-based regimens, respectively. Patients in Pt-based regimens arm were associated with significant improved DFS (HR = 0.70, 95% CI: 0.58–0.84), and OS (HR = 0.78, 95% CI: 0.61–1.00). The survival benefits of DFS remained consistent in both the two strategies of Pt usage, either adding Pt to standard anthracyclines&taxanes based regimens (A&T + Pt), or combination of Pt and taxanes alone (TPt). The survival benefits also remained consistent in either neoadjuvant or adjuvant use of Pt. The present meta-analysis of RCTs revealed that Pt-based chemo-regimens could significantly improve both DFS and OS for eTNBC patients. Based on efficiency and toxicity, we recommend Pt-based regimens for eTNBC, especially the “A&T + Pt” mode if the toxicities are tolerable, which may lead TNBC therapy into a new era.
Dipolar spin relaxation of divacancy qubits in silicon carbide
Authors: Bulancea-Lindvall Oscar , Son Nguyen T. , Abrikosov Igor A. , Ivády Viktor
Published: December 2021

Divacancy spins implement qubits with outstanding characteristics and capabilities in an industrial semiconductor host. On the other hand, there are still numerous open questions about the physics of these important defects, for instance, spin relaxation has not been thoroughly studied yet. Here, we carry out a theoretical study on environmental spin-induced spin relaxation processes of divacancy qubits in the 4H polytype of silicon carbide (4H-SiC). We reveal all the relevant magnetic field values where the longitudinal spin relaxation time T1 drops resonantly due to the coupling to either nuclear spins or electron spins. We quantitatively analyze the dependence of the T1 time on the concentration of point defect spins and the applied magnetic field and provide an analytical expression. We demonstrate that dipolar spin relaxation plays a significant role both in as-grown and ion-implanted samples and it often limits the coherence time of divacancy qubits in 4H-SiC.
Direct foam writing in microgravity
Authors: Cordonier Guy Jacob , Sharafati Cicely , Mays Spencer , Thackery Lukas , Gemmen Ellena , Cyphert Samuel , Brown Megan , Napolillo John Quinn , Toney Savannah , Moore Hunter , Kuhlman John M. , Sierros Konstantinos A.
Published: December 2021

Herein we report 2D printing in microgravity of aqueous-based foams containing metal oxide nanoparticles. Such hierarchical foams have potential space applications, for example for in situ habitat repair work, or for UV shielding. Foam line patterns of a TiO2-containing foam have been printed onto glass substrates via Direct Foam Writing (DFW) under microgravity conditions through a parabolic aircraft flight. Initial characterization of the foam properties (printed foam line width, bubble size, etc.) are presented. It has been found that gravity plays a significant role in the process of direct foam writing. The foam spread less over the substrate when deposited in microgravity as compared to Earth gravity. This had a direct impact on the cross-sectional area and surface roughness of the printed lines. Additionally, the contact angle of deionized water on a film exposed to microgravity was higher than that of a film not exposed to microgravity, due to the increased surface roughness of films exposed to microgravity.
Passive longitudinal weight and cardiopulmonary monitoring in the home bed
Authors: Harrington Nicholas , Bui Quan M. , Wei Zhe , Hernandez-Pacheco Brandon , DeYoung Pamela N. , Wassell Andrew , Duwaik Bayan , Desai Akshay S. , Bhatt Deepak L. , Agnihotri Parag , Owens Robert L. , Coleman Todd P. , King Kevin R.
Published: December 2021

Home health monitoring has the potential to improve outpatient management of chronic cardiopulmonary diseases such as heart failure. However, it is often limited by the need for adherence to self-measurement, charging and self-application of wearables, or usage of apps. Here, we describe a non-contact, adherence-independent sensor, that when placed beneath the legs of a patient’s home bed, longitudinally monitors total body weight, detailed respiratory signals, and ballistocardiograms for months, without requiring any active patient participation. Accompanying algorithms separate weight and respiratory signals when the bed is shared by a partner or a pet. Validation studies demonstrate quantitative equivalence to commercial sensors during overnight sleep studies. The feasibility of detecting obstructive and central apneas, cardiopulmonary coupling, and the hemodynamic consequences of non-sustained ventricular tachycardia is also established. Real-world durability is demonstrated by 3 months of in-home monitoring in an example patient with heart failure and ischemic cardiomyopathy as he recovers from coronary artery bypass grafting surgery. BedScales is the first sensor to measure adherence-independent total body weight as well as longitudinal cardiopulmonary physiology. As such, it has the potential to create a multidimensional picture of chronic disease, learn signatures of impending hospitalization, and enable optimization of care in the home.
Older age increases the risk of revision and perioperative complications after high tibial osteotomy for unicompartmental knee osteoarthritis
Authors: Lee Sun-Ho , Seo Hyoung-Yeon , Kim Hae-Rim , Song Eun-Kyoo , Seon Jong-Keun
Published: December 2021

Among various patient risk factors affecting survival after high tibial osteotomy (HTO), the ideal age limit for HTO is unclear. This study was performed to evaluate the effect of age on survival rate and complications after HTO for medial unicompartmental osteoarthritis. Among of 61,145 HTO patients from Korean National Health Insurance database, 41,112 patients underwent the procedure before the age of 60 years (Group A), 13,895 patients between the age of 60 and 65 years (Group B), and 6138 patients after the age of 65 years (Group C). We compared the survival rate in person-years among the three groups from the date of primary surgery until subsequent total knee arthroplasty. Perioperative complications were also recorded. The adjusted hazard ratio (HR) were calculated using the multivariable Cox proportional hazard regression model, adjusting for the potential confounders: age, sex, type of medical insurance, region of residence, hospital type, comorbidities, and Charlson comorbidity index score. The total number of HTO increased 6.5-fold, especially in patients aged > 65 years (by 8.2-fold) from 2008 to 2018. The overall revision rate was 4.2% in Group A, 6.4% in Group B, and 7.3% in Group C. The 5- and 10-year revision rate was significantly lower in Group A (p < 0.001), but no difference between Groups B and C. After adjusting for potential confounders, multivariable regression analysis revealed that revision rate was significantly lower in Group A than Group B (HR: 0.57; p < 0.0001), but no difference between Groups B and C. The incidence of complications was also significantly lower in Group A than in other groups. The inferior survival rate and more perioperative complications after HTO was found in old patients (aged ≥ 60 years) than in young patients. Therefore, the patient age is one of the predicting factors for a high risk of failure after HTO.
A customised target capture sequencing tool for molecular identification of Aloe vera and relatives
Authors: Woudstra Yannick , Viruel Juan , Fritzsche Martin , Bleazard Thomas , Mate Ryan , Howard Caroline , Rønsted Nina , Grace Olwen M.
Published: December 2021

Plant molecular identification studies have, until recently, been limited to the use of highly conserved markers from plastid and other organellar genomes, compromising resolution in highly diverse plant clades. Due to their higher evolutionary rates and reduced paralogy, low-copy nuclear genes overcome this limitation but are difficult to sequence with conventional methods and require high-quality input DNA. Aloe vera and its relatives in the Alooideae clade (Asphodelaceae, subfamily Asphodeloideae) are of economic interest for food and health products and have horticultural value. However, pressing conservation issues are increasing the need for a molecular identification tool to regulate the trade. With > 600 species and an origin of ± 15 million years ago, this predominantly African succulent plant clade is a diverse and taxonomically complex group for which low-copy nuclear genes would be desirable for accurate species discrimination. Unfortunately, with an average genome size of 16.76 pg, obtaining high coverage sequencing data for these genes would be prohibitively costly and computationally demanding. We used newly generated transcriptome data to design a customised RNA-bait panel targeting 189 low-copy nuclear genes in Alooideae. We demonstrate its efficacy in obtaining high-coverage sequence data for the target loci on Illumina sequencing platforms, including degraded DNA samples from museum specimens, with considerably improved phylogenetic resolution. This customised target capture sequencing protocol has the potential to confidently indicate phylogenetic relationships of Aloe vera and related species, as well as aid molecular identification applications.
Multi-step ahead predictive model for blood glucose concentrations of type-1 diabetic patients
Authors: Zaidi Syed Mohammed Arshad , Chandola Varun , Ibrahim Muhanned , Romanski Bianca , Mastrandrea Lucy D. , Singh Tarunraj
Published: December 2021

Continuous monitoring of blood glucose (BG) levels is a key aspect of diabetes management. Patients with Type-1 diabetes (T1D) require an effective tool to monitor these levels in order to make appropriate decisions regarding insulin administration and food intake to keep BG levels in target range. Effectively and accurately predicting future BG levels at multi-time steps ahead benefits a patient with diabetes by helping them decrease the risks of extremes in BG including hypo- and hyperglycemia. In this study, we present a novel multi-component deep learning model that predicts the BG levels in a multi-step look ahead fashion. The model is evaluated both quantitatively and qualitatively on actual blood glucose data for 97 patients. For the prediction horizon (PH) of 30 mins, the average values for root mean squared error (RMSE), mean absolute error (MAE), mean absolute percentage error (MAPE), and normalized mean squared error (NRMSE) are $$23.22 \pm 6.39$$

23.22
±
6.39

mg/dL, 16.77 ± 4.87 mg/dL, $$12.84 \pm 3.68$$

12.84
±
3.68

and $$0.08 \pm 0.01$$

0.08
±
0.01

respectively. When Clarke and Parkes error grid analyses were performed comparing predicted BG with actual BG, the results showed average percentage of points in Zone A of $$80.17 \pm 9.20$$

80.17
±
9.20

and $$84.81 \pm 6.11,$$

84.81
±
6.11
,

respectively. We offer this tool as a mechanism to enhance the predictive capabilities of algorithms for patients with T1D.
Disclosing the bioactive metabolites involved in the in vitro anthelmintic effects of salt-tolerant plants through a combined approach using PVPP and HPLC-ESI-MSn
Authors: Oliveira Marta , Lima Caroline Sprengel , Ketavong Setha , Llorent-Martínez Eulogio J. , Hoste Hervé , Custódio Luísa
Published: December 2021

Strategies to reduce dependence on synthetic drugs for the treatment of gastrointestinal nematodes (GIN) infections in ruminants include the search for novel anthelmintic scaffolds on plants, yet salt-tolerant plants remain overlooked. This study aims to evaluate the in vitro anthelmintic properties of selected salt-tolerant plants against GIN, and identify the potential bioactive secondary metabolites involved. For that purpose, 80% acetone/water extracts were prepared from dried biomass of aerial organs of nine salt-tolerant plant species and tested againstHaemonchus contortusandTrichostrongylus colubriformisby the Larval Exsheathment Inhibition Assay (LEIA) and Egg Hatching Inhibition Assay (EHIA).Pistacia lentiscus, Limoniatrum monopetalum, Cladium mariscusandHelychrisum italicum picardiwere the most active in both GIN and life stages. To investigate the role of polyphenols in the anthelmintic activity, four selected extracts were treated with polyvinylpolypyrrolidone (PVPP), and non-treated and treated samples were further characterized by high-performance liquid chromatography with electrospray ionization mass spectrometric detection (HPLC-ESI-MSn). While polyphenols seem responsible for the EHIA properties, they are partially accountable to LEIA results. Several phenolics involved in the anthelmintic effects were identified and discussed. In sum, these species are rich sources of anthelmintic compounds and, therefore, are of major interest for nutraceutical and/or phytotherapeutic applications against GIN in ruminants.
Insomnia subtypes characterised by objective sleep duration and NREM spectral power and the effect of acute sleep restriction: an exploratory analysis
Authors: Kao Chien-Hui , D’Rozario Angela L. , Lovato Nicole , Wassing Rick , Bartlett Delwyn , Memarian Negar , Espinel Paola , Kim Jong-Won , Grunstein Ronald R. , Gordon Christopher J.
Published: December 2021

Insomnia disorder (ID) is a heterogeneous disorder with proposed subtypes based on objective sleep duration. We speculated that insomnia subtyping with additional power spectral analysis and measurement of response to acute sleep restriction may be informative in overall assessment of ID. To explore alternative classifications of ID subtypes, insomnia patients (n = 99) underwent two consecutive overnight sleep studies: (i) habitual sleep opportunity (polysomnography, PSG) and, (ii) two hours less sleep opportunity (electroencephalography, EEG), with the first night compared to healthy controls (n = 25). ID subtypes were derived from data-driven classification of PSG, EEG spectral power and interhemispheric EEG asymmetry index. Three insomnia subtypes with different sleep duration and NREM spectral power were identified. One subtype (n = 26) had shorter sleep duration and lower NREM delta power than healthy controls (short-sleep delta-deficient; SSDD), the second subtype (n = 51) had normal sleep duration but lower NREM delta power than healthy controls (normal-sleep delta-deficient; NSDD) and a third subtype showed (n = 22) no difference in sleep duration or delta power from healthy controls (normal neurophysiological sleep; NNS). Acute sleep restriction improved multiple objective sleep measures across all insomnia subtypes including increased delta power in SSDD and NSDD, and improvements in subjective sleep quality for SSDD (p = 0.03), with a trend observed for NSDD (p = 0.057). These exploratory results suggest evidence of novel neurophysiological insomnia subtypes that may inform sleep state misperception in ID and with further research, may provide pathways for personalised care.
Molecular characterization and overexpression of the difenoconazole resistance gene CYP51 in Lasiodiplodia theobromae field isolates
Authors: Wang Chenguang , Xu Luxi , Liang Xiaoyu , Wang Jing , Xian Xinwei , Zhang Yu , Yang Ye
Published: December 2021

Stem-end rot (SER) caused by Lasiodiplodia theobromae is an important disease of mango in China. Demethylation inhibitor (DMI) fungicides are widely used for disease control in mango orchards. The baseline sensitivity to difenoconazole of 138 L. theobromae isolates collected from mango in the field in 2019 was established by the mycelial growth rate method. The cross-resistance to six site-specific fungicides with different modes of action were investigated using 20 isolates randomly selected. The possible mechanism for L. theobromae resistance to difenoconazole was preliminarily determined through gene sequence alignment and quantitative real-time PCR analysis. The results showed that the EC50 values of 138 L. theobromae isolates to difenoconazole ranged from 0.01 to 13.72 µg/mL. The frequency of difenoconazole sensitivity formed a normal distribution curve when the outliers were excluded. Difenoconazole showed positive cross-resistance only with the DMI tebuconazole but not with non-DMI fungicides carbendazim, pyraclostrobin, fludioxonil, bromothalonil, or iprodione. Some multifungicide-resistant isolates of L. theobromae were found. Two amino acid substitutions (E209k and G207A) were found in the CYP51 protein, but they were unlikely to be related to the resistance phenotype. There was no alteration in the promoter region of the CYP51 gene. However, difenoconazole significantly increased the expression of the CYP51 gene in the resistant isolates compared to the susceptible isolates. These results are vital to develop effective mango disease management strategies to avoid the development of further resistance.
Respiration monitoring in PACU using ventilation and gas exchange parameters
Authors: Kang Hee Yong , You Ann Hee , Kim Youngsoon , Jeong You Jeong , Jang Geuk Young , Oh Tong In , Kim Yongmin , Woo Eung Je
Published: December 2021

The importance of perioperative respiration monitoring is highlighted by high incidences of postoperative respiratory complications unrelated to the original disease. The objectives of this pilot study were to (1) simultaneously acquire respiration rate (RR), tidal volume (TV), minute ventilation (MV), SpO2 and PetCO2 from patients in post-anesthesia care unit (PACU) and (2) identify a practical continuous respiration monitoring method by analyzing the acquired data in terms of their ability and reliability in assessing a patient’s respiratory status. Thirteen non-intubated patients completed this observational study. A portable electrical impedance tomography (EIT) device was used to acquire RREIT, TV and MV, while PetCO2, RRCap and SpO2 were measured by a Capnostream35. Hypoventilation and respiratory events, e.g., apnea and hypopnea, could be detected reliably using RREIT, TV and MV. PetCO2 and SpO2 provided the gas exchange information, but were unable to detect hypoventilation in a timely fashion. Although SpO2 was stable, the sidestream capnography using the oronasal cannula was often unstable and produced fluctuating PetCO2 values. The coefficient of determination (R2) value between RREIT and RRCap was 0.65 with a percentage error of 52.5%. Based on our results, we identified RR, TV, MV and SpO2 as a set of respiratory parameters for robust continuous respiration monitoring of non-intubated patients. Such a respiration monitor with both ventilation and gas exchange parameters would be reliable and could be useful not only for respiration monitoring, but in making PACU discharge decisions and adjusting opioid dosage on general hospital floor. Future studies are needed to evaluate the potential clinical utility of such an integrated respiration monitor.
circRNA N6-methyladenosine methylation in preeclampsia and the potential role of N6-methyladenosine-modified circPAPPA2 in trophoblast invasion
Authors: Zhang Yonggang , Yang Hongling , Long Yan , Zhang Yipeng , Chen Ronggui , Shi Junzhu , Chen Jiying
Published: December 2021

Here, we performed N6-methyladenosine (m6A) RNA sequencing to determine the circRNA m6A methylation changes in the placentas during the pathogenesis of preeclampsia (PE). We verified the expression of the circRNA circPAPPA2 using quantitative reverse transcription-PCR. An invasion assay was carried out to identify the role of circPAPPA2 in the development of PE. Mechanistically, we investigated the cause of the altered m6A modification of circPAPPA2 through overexpression and knockdown cell experiments, RNA immunoprecipitation, fluorescence in situ hybridization and RNA stability experiments. We found that increases in m6A-modified circRNAs are prevalent in PE placentas and that the main changes in methylation occur in the 3’UTR and near the start codon, implicating the involvement of these changes in PE development. We also found that the levels of circPAPPA2 are decreased but that m6A modification is augmented. Furthermore, we discovered that methyltransferase‑like 14 (METTL14) increases the level of circPAPPA2 m6A methylation and that insulin-like growth factor 2 mRNA-binding protein 3 (IGF2BP3) maintains circPAPPA2 stability. Decreases in IGF2BP3 levels lead to declines in circPAPPA2 levels. In summary, we provide a new vision and strategy for the study of PE pathology and report that placental circRNA m6A modification appears to be an important regulatory mechanism.
Permanent pacemaker dependency in patients with new left bundle branch block and new first degree atrioventricular block after transcatheter aortic valve implantation
Authors: Hartrampf Bonnie , Jochheim David , Steffen Julius , Czermak Thomas , Sadoni Sebastian , Lemmermöhle Erik , Klier Ina , Estner Heidi L. , Massberg Steffen , Mehilli Julinda , Lackermair Korbinian , Fichtner Stephanie
Published: December 2021

Conduction disorders with need for permanent pacemaker (PPM) implantation remain frequent complications after transcatheter aortic valve implantation (TAVI). Up to 22% of PPM after TAVI are implanted for new onset left bundle branch block (LBBB) and atrioventricular block (AVB) I. However, clinical benefit and predictors of ventricular pacing in TAVI patients receiving PPM for this indication remain unclear. We retrospectively evaluated pacemaker interrogation data of patients who received a PPM post TAVI for new LBBB and new AVB I. The primary endpoint of this study was relevant ventricular pacing (ventricular pacing rate: Vp ≥ 1%) at the first outpatient pacemaker interrogation. Secondary endpoints were predictors for relevant ventricular pacing. At the first pacemaker interrogation (median follow up at 6.23 [2.8–14.8] months), median ventricular pacing frequency was 1.0% [0.1–17.8]. Out of 61 patients, 36 (59%) had Vp rates ≥ 1%. Patients with frequent ventricular pacing showed longer QRS duration (155 ms ± 17 ms vs. 144 ms ± 18 ms, p = 0.018) at the time of PPM implantation and were less likely treated with a balloon-expandable Edwards Sapiens Valve (39% vs. 12%, p = 0.040). Our findings suggest that the majority of patients with new LBBB and new AVB I after TAVI show relevant ventricular pacing rates at follow up. Further prospective studies are necessary to identify patients at higher risk of pacemaker dependency.
A bottom-up approach to construct or deconstruct a fluid instability
Authors: Escala Darío M. , Muñuzuri Alberto P.
Published: December 2021

Fluid instabilities have been the subject of study for a long time. Despite all the extensive knowledge, they still constitute a serious challenge for many industrial applications. Here, we experimentally consider an interface between two fluids with different viscosities and analyze their relative displacement. We designed the contents of each fluid in such a way that a chemical reaction takes place at the interface and use this reaction to suppress or induce a fingering instability at will. This process describes a road map to control viscous fingering instabilities in more complex systems via interfacial chemical reactions.