bluuebunny/crossref_metadata_2025 huggingface dataset:
Rollover Safety and Workable Boundary Suggestion of an Agricultural Platform with Different Attachments
Authors: Kang Seokho , Kim Yeongsu , Park Hyunggyu , Kim Yonggik , Woo Seungmin , Uyeh Daniel Dooyum , Ha Yushin
Published: August 2022

Overturning accidents in agriculture offroad vehicles have been reported annually around the world. Safety structures, such as rollover prevention, have been adopted to mitigate these accidents. Despite this, accidents remain persistent but less fatal. This study investigated the safe driving boundary for a multi-purpose platform with attachments (cargo, pepper harvester). Mathematical formulations of the roll and pitch motions for multi-purpose platforms were created. The critical overturning angle, at five load levels for each machine (0, 50, 100, 150, 200 kg), was determined using Recurdyn, a dynamic analysis software. Regression analysis of each coordinate of the center of gravity was conducted to verify the most critical coordinate against gaining loads. The critical overturning angle for the X and Y directions was found. The cargo and pepper harvester had 28.64° and 21.04° of critical overturning angles in the Y direction, respectively, with a full loading state while climbing a steep hill. The regression model of the X, Y, and Z coordinates of CoG suggested that the most critical coordinate of the CoG against the loads was in the Y coordinate for the pepper harvester and the X direction for cargo. This study could be applied to develop an awareness safety system that warns the operator of the risk of a fatal accident.
Hierarchical DDPG for Manipulator Motion Planning in Dynamic Environments
Authors: Um Dugan , Nethala Prasad , Shin Hocheol
Published: August 2022

In this paper, a hierarchical reinforcement learning (HRL) architecture, namely a “Hierarchical Deep Deterministic Policy Gradient (HDDPG)” has been proposed and studied. A HDDPG utilizes manager and worker formation similar to other HRL structures. However, unlike others, the HDDPG enables sharing an identical environment and state among workers and managers, while a unique reward system is required for each Deep Deterministic Policy Gradient (DDPG) agent. Therefore, the HDDPG allows easy structural expansion with probabilistic action selection of a worker by the manager. Due to its innate structural advantage, the HDDPG has a merit in building a general AI to deal with a complex time-horizon tasks with various conflicting sub-goals. The experimental results demonstrated its usefulness with a manipulator motion planning problem in a dynamic environment, where path planning and collision avoidance conflict each other. The proposed HDDPG is compared with an HAM and a single DDPG for performance evaluation. The result shows that the HDDPG demonstrated more than 40% of reward gain and more than two times the reward improvement rate. Another important feature of the proposed HDDPG is the biased manager training capability. By adding a preference factor to each worker, the manager can be trained to prefer a certain worker to achieve better success rate for a specific objective if needed.
Real-World Data on Antibiotic Group Treatment in European Livestock: Drivers, Conditions, and Alternatives
Authors: Jerab Julia , Jansen Wiebke , Blackwell John , van Hout Jobke , Palzer Andreas , Lister Stephen , Chantziaras Ilias , Dewulf Jeroen , De Briyne Nancy
Published: August 2022

Major efforts have been made by veterinary professionals to reduce the need for antibiotic use in animals. An online survey launched by the Federation of Veterinarians of Europe (FVE) aimed to gather responses from practicing veterinarians with field experience in metaphylactic livestock group treatment. Only 17% of all veterinarians (n = 183/1087, all species-specific responses merged) applied metaphylactic group treatments to 75% or more of all their treatments. Significantly less metaphylactic group treatments were reported in mixed practices (p = 0.002) and practices specialized in cattle (p < 0.001) as well as small (p = 0.007) and very small practices (p = 0.009). Gram-negative bacteria, mostly composed of Enterobacteriaceae and Pasteurellaceae, were considered by 75.3% (n = 967/1385) as the most devastating bacterial pathogens. Respondents alleged morbidity (20.1%, n = 201/998) and mortality (42.2%, n = 421/998) as major consequences for animal health and welfare if metaphylaxis would be banned. Responding veterinarians pointed towards vaccinations; improved biosecurity, including hygiene measures; and improved herd health management as the three most effective alternative measures to prevent metaphylactic treatment. However, more research is needed on how to implement appropriate alternatives in a holistic hurdle approach. Active support on a national level will be necessary for the development and application of targeted veterinary treatment guidelines for practitioners, which promote the understanding of drivers and include initiation criteria for metaphylactic group treatments in livestock.
Nordic Vets against AMR—An Initiative to Share and Promote Good Practices in the Nordic–Baltic Region
Authors: Sternberg-Lewerin Susanna , Boqvist Sofia , Nørstebø Simen Foyn , Grönthal Thomas , Heikinheimo Annamari , Johansson Venla , Heljanko Viivi , Kurittu Paula , Fall Nils , Magnusson Ulf , Mohn Bjelland Ane , Sørum Henning , Wasteson Yngvild
Published: August 2022

In the Nordic countries, antimicrobial use in animals and the prevalence of antimicrobial resistance are among the lowest in Europe. The network “Nordic Vets Against AMR” organized a meeting in 2021, with key actors including representatives from universities, veterinary authorities and veterinary organizations in Finland, Norway and Sweden. This paper reflects the most important discussions on education, research, policy and future perspectives, including the experiences of these countries. It concludes that Nordic veterinarians are well placed to lead the way in the fight against antimicrobial resistance and that the sharing of experiences can support colleagues in other countries. Veterinary education must go hand in hand with research activities and continuously updated guidelines and legislation. There is also a need for postgraduate training on antimicrobial resistance and prudent antimicrobial use. The veterinary profession must, by any means necessary, protect the efficiency of antimicrobials for the sake of animal health, animal welfare and productivity, as well as public health. While restrictive use of antimicrobials is crucial, the ability of veterinarians to use this medical tool is also important for the sake of animal welfare and global food security.
Incidence and Risk Factors for Blood Stream Infection in Mechanically Ventilated COVID-19 Patients
Authors: Mantzarlis Konstantinos , Deskata Konstantina , Papaspyrou Dimitra , Leontopoulou Vassiliki , Tsolaki Vassiliki , Zakynthinos Epaminondas , Makris Demosthenes
Published: August 2022

It is widely known that blood stream infections (BSIs) in critically ill patients may affect mortality, length of stay, or the duration of mechanical ventilation. There is scarce data regarding blood stream infections in mechanically ventilated COVID-19 patients. Preliminary studies report that the number of secondary infections in COVID-9 patients may be higher. This retrospective analysis was conducted to determine the incidence of BSI. Furthermore, risk factors, mortality, and other outcomes were analyzed. The setting was an Intensive Care Unit (ICU) at a University Hospital. Patients suffering from SARS-CoV-2 infection and requiring mechanical ventilation (MV) for >48 h were eligible. The characteristics of patients who presented BSI were compared with those of patients who did not present BSI. Eighty-four patients were included. The incidence of BSI was 57%. In most cases, multidrug-resistant pathogens were isolated. Dyslipidemia was more frequent in the BSI group (p < 0.05). Moreover, BSI-group patients had a longer ICU stay and a longer duration of both mechanical ventilation and sedation (p < 0.05). Deaths were not statistically different between the two groups (73% for BSI and 56% for the non-BSI group, p > 0.05). Compared with non-survivors, survivors had lower baseline APACHE II and SOFA scores, lower D-dimers levels, a higher baseline compliance of the respiratory system, and less frequent heart failure. They received anakinra less frequently and appropriate therapy more often (p < 0.05). The independent risk factor for mortality was the APACHE II score [1.232 (1.017 to 1.493), p = 0.033].
Antibiotic Allergy De-Labeling: A Pathway against Antibiotic Resistance
Authors: Doña Inmaculada , Labella Marina , Bogas Gádor , Sáenz de Santa María Rocío , Salas María , Ariza Adriana , Torres María José
Published: August 2022

Antibiotics are one of the most frequently prescribed drugs. Unfortunately, they also are the most common cause for self-reported drug allergy, limiting the use of effective therapies. However, evidence shows that more than 90% of patients labeled as allergic to antibiotics are not allergic. Importantly, the label of antibiotic allergy, whether real or not, constitutes a major public health problem as it directly impacts antimicrobial stewardship: it has been associated with broad-spectrum antibiotic use, often resulting in the emergence of bacterial resistance. Therefore, an accurate diagnosis is crucial for de-labeling patients who claim to be allergic but are not really allergic. This review presents allergy methods for achieving successful antibiotic allergy de-labeling. Patient clinical history is often inaccurately reported, thus not being able to de-label most patients. In vitro testing offers a complementary approach but it shows limitations. Immunoassay for quantifying specific IgE is the most used one, although it gives low sensitivity and is limited to few betalactams. Basophil activation test is not validated and not available in all centers. Therefore, true de-labeling still relies on in vivo tests including drug provocation and/or skin tests, which are not risk-exempt and require specialized healthcare professionals for results interpretation and patient management. Moreover, differences on the pattern of antibiotic consumption cause differences in the diagnostic approach among different countries. A multidisciplinary approach is recommended to reduce the risks associated with the reported penicillin allergy label.
Modulation of 1,2-Dicarbonyl Compounds in Postprandial Responses Mediated by Food Bioactive Components and Mediterranean Diet
Authors: Cruz Nadia , Flores Marcos , Urquiaga Inés , Ávila Felipe
Published: August 2022

Glycoxidative stress with the consequent generation of advanced glycation end products has been implied in the etiology of numerous non-communicable chronic diseases. During the postprandial state, the levels of 1,2-dicarbonyl compounds can increase, depending on numerous factors, including characteristics of the subjects mainly related to glucose metabolism disorders and nutritional status, as well as properties related to the chemical composition of meals, including macronutrient composition and the presence of dietary bioactive molecules and macromolecules. In this review, we examine the chemical, biochemical, and physiological pathways that contribute to postprandial generation of 1,2-dicarbonyl compounds. The modulation of postprandial 1,2-dicarbonyl compounds is discussed in terms of biochemical pathways regulating the levels of these compounds, as well as the effect of phenolic compounds, dietary fiber, and dietary patterns, such as Mediterranean and Western diets.
Redox Homeostasis in Ocular Tissues: Circadian Regulation of Glutathione in the Lens?
Authors: Lim Julie C. , Suzuki-Kerr Haruna , Nguyen Tai X. , Lim Christopher J. J. , Poulsen Raewyn C.
Published: August 2022

Accumulating evidence in tissues suggests an interconnection between circadian clocks and redox regulation. Diurnal variations in antioxidant levels, circadian rhythms of antioxidant enzyme activity, and differences in oxidative stress markers at different times of the day all indicate that oxidative stress responses follow a circadian rhythm. Disruptions of circadian rhythms are linked to a number of age-related diseases, including those in the eye. Typically, ocular tissues contain a robust antioxidant defence system to maintain redox balance and minimise oxidative stress and damage. The lens, in particular, contains remarkably high levels of the antioxidant glutathione (GSH). However, with advancing age, GSH levels deplete, initiating a chain of biochemical events that ultimately result in protein aggregation, light scattering, and age-related cataracts. While there is evidence that the lens exhibits circadian rhythms in the synthesis and release of melatonin, little is known about the regulation or function of timekeeping mechanisms in the lens. Since circadian rhythms are disrupted with age, and the depletion of GSH in the lens is a known initiating factor in the development of age-related cataracts, understanding the mechanisms involved in regulating GSH levels may lead to the future development of approaches to manipulate the clock to restore GSH levels and redox balance in the lens, and protect the lens from cataracts.
Impact of Gastrointestinal Digestion on the Anti-Inflammatory Properties of Phlorotannins from Himanthalia elongata
Authors: Catarino Marcelo D. , Circuncisão Ana Rita , Neves Bruno , Marçal Catarina , Silva Artur M. S. , Cruz Maria Teresa , Cardoso Susana M.
Published: August 2022

A phlorotannin extract was obtained from Himanthalia elongata, revealing a profile rich in fucophlorethol-type and carmalol-type compounds. When subjected to simulated gastrointestinal digestion, its levels of total phlorotannins and antioxidant activity, measured in vitro via NO● and O2●− scavenging assays, were reduced, thus suggesting that these compounds’ integrity and bioactivity are negatively affected by the digestive process. Nevertheless, when undigested vs. digested extracts were used on lipopolysaccharide-stimulated Raw 264.7 macrophages, both showed a strong inhibitory effect on the cellular NO● production. In fact, although not statistically significant, the digested extract revealed a tendentially stronger effect compared to its undigested counterpart, suggesting that even though there is a decrease in the phlorotannins’ concentration after digestion, with a consequent loss of their scavenging properties, the possible degradation products being formed may exert their effects through the modulation of the intracellular signaling mechanisms. Overall, this study not only contributes to a better understanding of the phlorotannins’ composition of the species H. elongata, but also shows that, although the digestive process may affect the integrity and concentration of these compounds, this does not necessarily translate into loss of bioactivity, in particular the anti-inflammatory activity, probably owing to the bioactive effects that the degradation products of these phenolics may have at an intracellular level.
Estimating the Soil-Water Retention Curve of Arsenic-Contaminated Soil by Fitting Fuentes’ Model and Their Comparison with the Filter Paper Method
Authors: Vásquez-Nogal Ismael , Hernández-Mendoza Christian E. , Cárdenas-Robles Arely I. , Rojas-González Eduardo
Published: August 2022

Arsenic is a metalloid frequently found in contaminated sites, especially in the soil. In this regard, soil contamination has attracted researchers’ attention because it affects soil suction, which is essential in unsaturated soil mechanics. Due to its reliability and low cost, the filter paper method is frequently used to evaluate soil suction. However, it is laborious and time consuming. As an alternative, different mathematical models have been developed to estimate natural soil’s suction. The objective of this study was to elucidate if Fuentes’ model (using fractal, Mualem, and Burdine restrictions) can be used to estimate the soil-water retention curve of an arsenic-contaminated soil by comparing it with the filter paper method data already reported. The results showed that under natural conditions, Fuentes’ model provided similar results to those obtained by the filter paper method. The model also suggested modification of the soil’s structure, observed by the increase in the soil’s particle diameter after contamination. However, Fuentes’ model was observed to overestimate the suction values for contaminated soils. This observation highlights the necessity to use a soil stabilization method to control the soil’s volume variations. The data also showed that Fuentes’ model overestimated the hydraulic conductivity function of the contaminated soil. Hence, the retention potential does not reflect the behavior of the contaminated soils and can induce misinterpretation of contaminant transport evaluation in soil. Nevertheless, further analyses should be performed to investigate the direct applicability of this model to soils contaminated with other substances.
Stereotactic Positioning System: Towards a Mechanism Used in Thermal Ablation Therapy
Authors: Mendez Maria Jose , Lara Hernandez Gemima , Trujillo-Romero Citlalli Jessica , Martinez Sibaja Albino , Flores Cuautle Jose Jesus Agustín
Published: August 2022

In microwave thermal ablation, placing the antenna on a specific coordinate is one of the most critical steps. Several stereotactic systems can place an instrument on a specific point with great accuracy. However, these systems are developed for neurosurgery; moreover, a stereotactic system used in microwave thermal ablation must not disturb the electromagnetic (EM) pattern generated by the antenna. A stereotactic positioning system was designed, built, and tested. Different types of materials were proposed to manufacture the proposed stereotactic system to locate the microwave antennas. The stereotactic system can displace the microwave antenna around the Z-axis and Theta-axis. Displacements were generated by stepper motors and controlled by the user through a graphical interface. The system tests consist of programming displacements along the two axes in steps of 5 mm on the Z-axis and 5 degrees on the Theta-axis. Results showed that the system is capable of moving using cylindrical coordinates over a 70 mm displacement with an average error of ±0.85 mm for sensors on the Z-axis, while in the Theta-axis it reaches 180∘ displacement with an error of ±2.64∘. A stereotactic microwave antenna positioning system was developed and preliminarily tested. This first system can already be used to evaluate antenna performance either in phantoms or ex-vivo tissue. Moreover, this system can be extrapolated to different parts of the human body and be adapted to the required dimensions.
Study of Crack Growth of Transparent Materials Subjected to Laser Irradiation by Digital Holography
Authors: Zhou Wenjing , Liu Yuhang , Chen Zhenkai , Chen Yao , Zhang Hongbo , Yu Yingjie , Tornari Vivi
Published: August 2022

The crack growth of transparent materials after laser wavelength irradiation was studied. It is known that laser irradiation is used in many applications for the ablation of undesired material and/or coatings. The impact of laser irradiation on cracks was studied using the digital holography (DH) technique. Transparent samples were irradiated using near-ultraviolet, visible, near-infrared, and infrared light. The DH system is able to detect cracks and crack growth of the transparent samples irradiated by a range of laser wavelengths. Results also show that light with infrared to near-infrared wavelengths has a great effect on crack growth. High-resolution photomechanical effects of laser irradiation on material expansion or/and generation of defects due to specific wavelengths are also illustrated. The DH system with a multispectral laser has practical applications for laser cleaning of painted artworks.
Effect of the Cadmium Telluride Deposition Method on the Covering Degree of Electrodes Based on Copper Nanowire Arrays
Authors: Panaitescu Ana-Maria , Antohe Iulia , Locovei Claudiu , Iftimie Sorina , Antohe Ştefan , Piraux Luc , Suchea Mirela Petruta , Antohe Vlad-Andrei
Published: August 2022

In this work, we report the preparation of nanostructured electrodes based on dense arrays of vertically-aligned copper (Cu) nanowires (NWs) to be subsequently covered by cadmium telluride (CdTe) thin films, with great potential to be used within “substrate”-type photovoltaic cells based on AII-BVI heterojunctions. In particular, the multi-step preparation protocol presented here involves an electrochemical synthesis procedure within a supported anodic aluminum oxide (AAO) nanoporous template for first generating a homogeneous array of vertically-aligned Cu NWs, which are then further embedded within a compact CdTe thin film. In a second stage, we tested three deposition methods (vacuum thermal evaporation, VTE; radio-frequency magnetron sputtering, RF-MS; and electrochemical deposition, ECD) for use in obtaining CdTe layers potentially able to consistently penetrate the previously prepared Cu NWs array. A comparative analysis was performed to critically evaluate the morphological, optical, and structural properties of the deposited CdTe films. The presented results demonstrate that under optimized processing conditions, the ECD approach could potentially allow the cost-effective fabrication of absorber layer/collecting electrode CdTe/Cu nanostructured interfaces that could improve charge collection mechanisms, which in turn could allow the fabrication of more efficient solar cells based on AII-BVI semiconducting compounds.
Experimental, Numerical, and Theoretical Studies of Bond Behavior of Reinforced Fly Ash-Based Geopolymer Concrete
Authors: Le Tuan Anh , Nguyen Thuy Ninh , Nguyen Khoa Tan
Published: August 2022

This paper presents the results of an experiment study and suggests a theoretical formulation for the bond behavior of reinforced fly ash-based geopolymer concrete. Three grades (20 MPa, 30 MPa and 40 MPa) of a geopolymer concrete along with three reinforcement diameters (12, 16, and 20 mm) were selected for experimental work. The bond behavior of the reinforced geopolymer concrete is determined using pullout test, finite element analysis (FEA), and theoretical work. The test data indicated that the bond strength of reinforced fly ash-based geopolymer concrete increases about 1.97 to 2.56 times with the increase of compressive strength from 20.33 MPa to 41.12 MPa. For grade 30 MPa and 40 MPa specimens, the concrete cover to diameter ratio (c/db) increased up to 4.19 resulted in the increase of bond strength. Then, the bond strength decreases with the increase of the c/db ratio from 4.19 to 5.75, while grade 20 MPa specimens is vice versa. The bond-slip relations between the reinforcement and geopolymer concrete determined from FEA is in good agreement with experimental results. The coefficient of variation (CoV) is only 0.01. However, this behavior is quite different from the data calculated by the fib model.
BODIPY-Based Nanomaterials—Sensing and Biomedical Applications
Authors: Koczorowski Tomasz , Glowacka-Sobotta Arleta , Sysak Stepan , Mlynarczyk Dariusz T. , Lesyk Roman , Goslinski Tomasz , Sobotta Lukasz
Published: August 2022

Cancerous diseases are rightfully considered among the most lethal, which have a consistently negative effect when considering official statistics in regular health reports around the globe. Nowadays, metallic nanoparticles can be potentially applied in medicine as active pharmaceuticals, adjustable carriers, or distinctive enhancers of physicochemical properties if combined with other drugs. Boron dipyrromethene (BODIPY) molecules have been considered for future applications in theranostics in the oncology field, thus expanding the potential of conceivable applicability. Hence, taking into account positive practical features of both metal-based nanostructures and BODIPY derivatives, the present study aims to gather recent results connected to BODIPY-conjugated metallic nanoparticles. This is with respect to their expediency in the diagnosis and treatment of tumor ailments as well as in sensing of heavy metals. To fulfill the designated objectives, multiple research documents were analyzed concerning the latest discoveries within the scope of BODIPY-based nanomaterials with particular emphasis on their utilization for diagnostical sensing as well as cancer diagnostics and therapy. In addition, collected examples of mentioned conjugates were presented in order to draw the attention of the scientific community to their practical applications, elucidate the topic in a consistent manner, and inspire fellow researchers for new findings.
Bird Cherry (Prunus padus) Fruit Extracts Inhibit Lipid Peroxidation in PC Liposomes: Spectroscopic, HPLC, and GC–MS Studies
Authors: Siejak Przemysław , Smułek Wojciech , Nowak-Karnowska Joanna , Dembska Anna , Neunert Grażyna , Polewski Krzysztof
Published: August 2022

The antioxidant potential of bird cherry fruit of water, methanol, ethanol, and acetone extracts and their antioxidant efficiency against oxidation of PC liposomes using spectroscopic and chromatographic methods were investigated. The chromatographic methods quantified and specified the presence of phenolic and flavonoid compounds in the investigated extracts. The characteristic peaks in the UV spectrum at 275 nm and 370 nm confirmed the presence of phenols and flavonoids and their derivatives. Their presence was also confirmed by FTIR spectra, which revealed the presence of its functional groups. The total luminescence spectra with maxima at 314–318 nm, 325–355 nm, and 428–435 nm were ascribed to the presence of phenolic acids and tocopherols. The antioxidant properties of extracts and its inhibition properties against lipid peroxidation in PC liposomes were determined by fluorogenic probes DCF-H and C11-BODIPY581/591. The measured antioxidant properties against generated free radicals in aqueous and lipogenic phases revealed differences between extracts depending on their physicochemical properties with the greatest potential for acetone extract and sirup. The presented quantitative analysis indicated that cherry bird extracts possess significant amounts of phenolics and flavonoids, thus having the opportunity to be used as a natural antioxidant agent source with a large potential for application in pharmaceutical and food industries.
Experimental and Numerical Investigation of a Multifunctional CFRP towards Heat Convection under Aircraft Icing Conditions
Authors: Schutzeichel Maximilian Otto Heinrich , Strübing Thorben , Tamer Ozan , Kletschkowski Thomas , Monner Hans Peter , Sinapius Michael
Published: August 2022

A combined experimental and numerical approach for the analysis of convective heat transfer from a multifunctional flat plate specimen under aircraft icing conditions is presented. The experimental setup including a heat control and measurement system that is installed in a de-icing test bed. The ambient temperature (θa=[253,283]K), air velocity (va={0,15,30}ms), and angle of attack (α={10,30}∘) are varied, and their influence on heat transfer during local Joule heating is discussed. The numerical approach utilises the results to compute the convective heat transfer coefficients (HTC) based on Newton’s convective heat transfer condition. Results indicate that the numerical model represents the heat transfer behaviour with high accuracy. The HTC for free convection was found to hold h¯≈2.5Wm2K and h¯≈[10,40]Wm2K for forced convection conditions with minor scattering. The increase in HTC under forced convection conditions has a significant effect on the overall heat transfer behaviour, resulting in high temperature gradients within the material. The functional optimisation of multifunctional structures will benefit from including application related convection conditions, dealing with resulting temperature fields by structural design. It is expected that multifunctional structures for de-icing as well as for structural energy storage, morphing structures, or stiffness adaptive structures with similar material constituents will benefit from this recognition.
GIS-Based Spatial Modeling of Snow Avalanches Using Analytic Hierarchy Process. A Case Study of the Šar Mountains, Serbia
Authors: Durlević Uroš , Valjarević Aleksandar , Novković Ivan , Ćurčić Nina B. , Smiljić Mirjana , Morar Cezar , Stoica Alina , Barišić Danijel , Lukić Tin
Published: August 2022

Snow avalanches are one of the most devastating natural hazards in the highlands that often cause human casualties and economic losses. The complex process of modeling terrain susceptibility requires the application of modern methods and software. The prediction of avalanches in this study is based on the use of geographic information systems (GIS), remote sensing, and multicriteria analysis—analytic hierarchy process (AHP) on the territory of the Šar Mountains (Serbia). Five indicators (lithological, geomorphological, hydrological, vegetation, and climatic) were processed, where 14 criteria were analyzed. The results showed that approximately 20% of the investigated area is highly susceptible to avalanches and that 24% of the area has a medium susceptibility. Based on the results, settlements where avalanche protection measures should be applied have been singled out. The obtained data can will help local self-governments, emergency management services, and mountaineering services to mitigate human and material losses from the snow avalanches. This is the first research in the Republic of Serbia that deals with GIS-AHP spatial modeling of snow avalanches, and methodology and criteria used in this study can be tested in other high mountainous regions.
A Real-Time Approach to Detect PM2.5 in a Seriously Polluted Environment Based on Pressure Drop
Authors: Li Jialin , Zheng Lina , Xuan Peng , Huo Ruiyan
Published: August 2022

A differential pressure-based low-cost PM2.5 detection system was developed for particulate matter measurement in polluted environments. The PM2.5 monitor consists of a sampling device, a pump, a pressure sensor, and a control circuit. Two sampling devices including a foam penetration-filter tube and a cyclone-filter holder were applied. Tests were conducted in a haze environment and laboratory particle chambers with varying PM2.5 concentration. The pressure data were related to the PM2.5 concentration recorded by Dusttrak to show the calibration process and the performance of this instrument. Results showed the concentration information given by the instrument was consistent with the actual concentration in the experiment, and this instrument was more suitable for seriously polluted environment detection. Concentration oscillation of the pressure-based PM2.5 monitor caused by turbulent flow could be reduced by a longer calculation interval and data averaging in the calculation process. As a low-cost sensor, the pressure-based PM2.5 monitor still has good performance and application value for detecting high-concentration PM2.5 in atmospheric environments or workplaces.
Effects on Muscular Activity after Surgically Assisted Rapid Palatal Expansion: A Prospective Observational Study
Authors: Farronato Marco , Farronato Davide , Giannì Aldo Bruno , Inchingolo Francesco , Nucci Ludovica , Tartaglia Gianluca Martino , Maspero Cinzia
Published: August 2022

The study aims to investigate the modifications in the temporalis and the masseter activity in adult patients before and after SARPE (Surgically Assisted Rapid Palatal Expansion) by measuring electromyographic and electrokinesographic activity. 24 adult patients with unilateral posterior crossbite on the right side were selected from the Orthodontic Department of the University of Milan. Three electromyographic and electrokinesographic surface readings were taken respectively before surgery (T0) and 8 months after surgery (T1). The electromyographic data of both right and left masseter and anterior temporalis muscles were recorded during multiple tests: standardized maximum voluntary contraction (MVC)s, after transcutaneous electrical nerve stimulation (TENS) and at rest. T0 and T1 values were compared with paired Student’s t-test (p < 0.05). Results: Significant differences were found in the activity of right masseter (p = 0.03) and right temporalis (p = 0.02) during clench, in the evaluation of right masseter at rest (p = 0.03), also the muscular activity of masseters at rest after TENS from T0 to T1 (pr = 0.04, pl = 0.04). No significant differences were found in the activity of left masseter (p = 0.41) and left temporalis (p = 0.39) during clench and MVC, in the evaluation of left masseter at rest (p = 0.57) and in the activity during MVC of right masseter (p = 0.41), left masseter (p = 0.34), right temporalis (p = 0.51) and left temporalis (p = 0.77). Results showed that the activity of the masseter and temporalis muscles increased significantly after SARPE during rest and clenching on the side where the cross-bite was treated.
Impact of a Pretreatment Step on the Acidogenic Fermentation of Spent Coffee Grounds
Authors: Pereira Joana , de Melo Marcelo M. R. , Silva Carlos M. , Lemos Paulo C. , Serafim Luísa S.
Published: August 2022

Acidogenic fermentation (AF) is often applied to wastes to produce short-chain organic acids (SCOAs)—molecules with applications in many industries. Spent coffee grounds (SCGs) are a residue from the coffee industry that is rich in carbohydrates, having the potential to be valorized by this process. However, given the recalcitrant nature of this waste, the addition of a pretreatment step can significantly improve AF. In this work, several pretreatment strategies were applied to SCGs (acidic hydrolysis, basic hydrolysis, hydrothermal, microwave, ultrasounds, and supercritical CO2 extraction), evaluated in terms of sugar and inhibitors release, and used in AF. Despite the low yields of sugar extracted, almost all pretreatments increased SCOAs production. Milder extraction conditions also resulted in lower concentrations of inhibitory compounds and, consequently, in a higher concentration of SCOAs. The best results were obtained with acidic hydrolysis of 5%, leading to a production of 1.33 gSCOAs/L, an increase of 185% compared with untreated SCGs.
Postharvest Operations of Cannabis and Their Effect on Cannabinoid Content: A Review
Authors: Das Pabitra Chandra , Vista Alec Roger , Tabil Lope G. , Baik Oon-Doo
Published: August 2022

In recent years, cannabis (Cannabis sativa L.) has been legalized by many countries for production, processing, and use considering its tremendous medical and industrial applications. Cannabis contains more than a hundred biomolecules (cannabinoids) which have the potentiality to cure different chronic diseases. After harvesting, cannabis undergoes different postharvest operations including drying, curing, storage, etc. Presently, the cannabis industry relies on different traditional postharvest operations, which may result in an inconsistent quality of products. In this review, we aimed to describe the biosynthesis process of major cannabinoids, postharvest operations used by the cannabis industry, and the consequences of postharvest operations on the cannabinoid profile. As drying is the most important post-harvest operation of cannabis, the attributes associated with drying (water activity, equilibrium moisture content, sorption isotherms, etc.) and the significance of novel pre-treatments (microwave heating, cold plasma, ultrasound, pulse electric, irradiation, etc.) for improvement of the process are thoroughly discussed. Additionally, other operations, such as trimming, curing, packaging and storage, are discussed, and the effect of the different postharvest operations on the cannabinoid yield is summarized. A critical investigation of the factors involved in each postharvest operation is indeed key for obtaining quality products and for the sustainable development of the cannabis industry.
Seasonal Variation of Midgut Bacterial Diversity in Culex quinquefasciatus Populations in Haikou City, Hainan Province, China
Authors: Suo Penghui , Wang Kaixuan , Yu Hongxiao , Fu Xiuhao , An Liping , Bhowmick Biswajit , Zhang Jiachao , Han Qian
Published: August 2022

Culex quinquefasciatus, one of the most significant mosquito vectors in the world, is widespread in most parts of southern China. A variety of diseases including Bancroft’s filariasis, West Nile disease, and St. Louis encephalitis could be transmitted by the vector. Mosquitoes have been shown to host diverse bacterial communities that vary depending on environmental factors such as temperature and rainfall. In this work, 16S rDNA sequencing was used to analyze the seasonal variation of midgut bacterial diversity of Cx. Quinquefasciatus in Haikou City, Hainan Province, China. Proteobacteria was the dominant phylum, accounting for 79.7% (autumn), 73% (winter), 80.4% (spring), and 84.5% (summer). The abundance of Bacteroidetes in autumn and winter was higher than in others. Interestingly, Epsilonbacteraeota, which only exists in autumn and winter, was discovered accidentally in the midgut. We speculated that this might participate in the nutritional supply of adult mosquitoes when temperatures drop. Wolbachia is the most abundant in autumn, accounting for 31.6% of bacteria. The content of Pantoea was highest in the summer group, which might be related to the enhancement of the ability of mosquitoes as temperatures increased. Pseudomonas is carried out as the highest level in winter. On the contrary, in spring and summer, the genus in highest abundance is Enterobacter. Acinetobacter enriches in the spring when it turns from cold to hot. By studying the diversity of midgut bacteria of Cx. quinquefasciatus, we can further understand the co-evolution of mosquitoes and their symbiotic microbes. This is necessary to discuss the seasonal variation of microorganisms and ultimately provide a new perspective for the control of Cx. quinquefasciatus to reduce the spread of the diseases which have notably vital practical significance for the effective prevention of Cx. quinquefasciatus.
Anti-Inflammatory microRNAs for Treating Inflammatory Skin Diseases
Authors: Yang Shih-Chun , Alalaiwe Ahmed , Lin Zih-Chan , Lin Yu-Chih , Aljuffali Ibrahim A. , Fang Jia-You
Published: August 2022

Skin inflammation occurs due to immune dysregulation because of internal disorders, infections, and allergic reactions. The inflammation of the skin is a major sign of chronic autoimmune inflammatory diseases, such as psoriasis, atopic dermatitis (AD), and lupus erythematosus. Although there are many therapies for treating these cutaneous inflammation diseases, their recurrence rates are high due to incomplete resolution. MicroRNA (miRNA) plays a critical role in skin inflammation by regulating the expression of protein-coding genes at the posttranscriptional level during pathogenesis and homeostasis maintenance. Some miRNAs possess anti-inflammatory features, which are beneficial for mitigating the inflammatory response. miRNAs that are reduced in inflammatory skin diseases can be supplied transiently using miRNA mimics and agomir. miRNA-based therapies that can target multiple genes in a given pathway are potential candidates for the treatment of skin inflammation. This review article offers an overview of the function of miRNA in skin inflammation regulation, with a focus on psoriasis, AD, and cutaneous wounds. Some bioactive molecules can target and modulate miRNAs to achieve the objective of inflammation suppression. This review also reports the anti-inflammatory efficacy of these molecules through modulating miRNA expression. The main limitations of miRNA-based therapies are rapid biodegradation and poor skin and cell penetration. Consideration was given to improving these drawbacks using the approaches of cell-penetrating peptides (CPPs), nanocarriers, exosomes, and low-frequency ultrasound. A formulation design for successful miRNA delivery into skin and target cells is also described in this review. The possible use of miRNAs as biomarkers and therapeutic modalities could open a novel opportunity for the diagnosis and treatment of inflammation-associated skin diseases.
G-Quadruplex Formed by the Promoter Region of the hTERT Gene: Structure-Driven Effects on DNA Mismatch Repair Functions
Authors: Pavlova Anzhela V. , Savitskaya Victoria Yu. , Dolinnaya Nina G. , Monakhova Mayya V. , Litvinova Anastasia V. , Kubareva Elena A. , Zvereva Maria I.
Published: August 2022

G-quadruplexes (G4s) are a unique class of noncanonical DNAs that play a key role in cellular processes and neoplastic transformation. Herein, we focused on the promoter region of human TERT oncogene, whose product is responsible for the immortality of cancer cells. It has been shown by chemical probing and spectroscopic methods that synthetic 96-nt DNAs modeling the wild-type G-rich strand of the hTERT promoter and its variants with G>A point substitutions corresponding to somatic driver mutations fold into three stacked parallel G4s with sites of local G4 destabilization caused by G>A substitutions in the G4 motif. These models were used to elucidate how the hTERT multiG4 affects the binding affinity and functional responses of two key proteins, MutS and MutL, involved in the initial stage of DNA mismatch repair (MMR) in Escherichiacoli and Neisseriagonorrhoeae with different MMR mechanisms. We have shown for the first time that (i) point substitutions do not affect the effective binding of these proteins to the hTERT G4 structure, and (ii) the endonuclease activity of MutL from N. gonorrhoeae is significantly suppressed by the stable G4 scaffold. It is likely that some of the genomic instability associated with G4 may be related to the blockage of human intrinsic methyl-independent MMR attempting to operate near G4 structures.
Soluble (Pro)Renin Receptor Levels Are Regulated by Plasma Renin Activity and Correlated with Edema in Mice and Humans with HFrEF
Authors: Gladysheva Inna P. , Sullivan Ryan D. , Ramanathan Kodangudi , Reed Guy L.
Published: August 2022

Symptomatic heart failure with reduced ejection fraction (HFrEF) is characterized by edema and chronic pathological activation of the classical renin–angiotensin–aldosterone system (RAAS). The soluble (pro)renin receptor (s(P)RR) is released into circulation by proteolytic cleavage of tissue expressed (P)RR and is a candidate biomarker of RAAS activation. However, previous studies linked elevated levels of s(P)RR in patients with HFrEF to renal dysfunction. Utilizing prospectively enrolled patients with comparable rEF, we show that increased plasma levels of s(P)RR are associated with symptomatic HF (characterized by edema), independent of chronic renal dysfunction. We also found that s(P)RR levels were positively correlated with patient plasma renin activity (PRA). Normotensive mice with dilated cardiomyopathy (DCM) and HFrEF, without renal dysfunction, showed plasma s(P)RR and PRA patterns similar to human HFrEF patients. Plasma s(P)RR levels positively correlated with PRA and systemic edema, but not with EF, resembling findings in patients with HFrEF without chronic kidney dysfunction. In female DCM mice with elevated PRA levels and plasma s(P)RR levels, a randomized, blinded trial comparing the direct renin inhibitor, aliskiren vs. vehicle control, showed that direct renin inhibition normalized PRA, lowered s(P)RR, and prevented symptomatic HFrEF. Considered in light of previous findings, these data suggest that, in HFrEF, in the absence of renal dysfunction, elevation of plasma s(P)RR levels is caused by increased PRA and associated with the development of systemic edema.
The Grasping Test Revisited: A Systematic Review of Functional Recovery in Rat Models of Median Nerve Injury
Authors: Lauer Henrik , Prahm Cosima , Thiel Johannes Tobias , Kolbenschlag Jonas , Daigeler Adrien , Hercher David , Heinzel Johannes C.
Published: August 2022

The rat median nerve model is a well-established and frequently used model for peripheral nerve injury and repair. The grasping test is the gold-standard to evaluate functional recovery in this model. However, no comprehensive review exists to summarize the course of functional recovery in regard to the lesion type. According to PRISMA-guidelines, research was performed, including the databases PubMed and Web of Science. Groups were: (1) crush injury, (2) transection with end-to-end or with (3) end-to-side coaptation and (4) isogenic or acellular allogenic grafting. Total and respective number, as well as rat strain, type of nerve defect, length of isogenic or acellular allogenic allografts, time at first signs of motor recovery (FSR) and maximal recovery grasping strength (MRGS), were evaluated. In total, 47 articles met the inclusion criteria. Group I showed earliest signs of motor recovery. Slow recovery was observable in group III and in graft length above 25 mm. Isografts recovered faster compared to other grafts. The onset and course of recovery is heavily dependent from the type of nerve injury. The grasping test should be used complementary in addition to other volitional and non-volitional tests. Repetitive examinations should be planned carefully to optimize assessment of valid and reliable data.
Plasma Phospho-Tau-181 as a Diagnostic Aid in Alzheimer’s Disease
Authors: Tsantzali Ioanna , Foska Aikaterini , Sideri Eleni , Routsi Evdokia , Tsomaka Effrosyni , Kitsos Dimitrios K. , Zompola Christina , Bonakis Anastasios , Giannopoulos Sotirios , Voumvourakis Konstantinos I. , Tsivgoulis Georgios , Paraskevas George P.
Published: August 2022

Cerebrospinal fluid (CSF) biomarkers remain the gold standard for fluid-biomarker-based diagnosis of Alzheimer’s disease (AD) during life. Plasma biomarkers avoid lumbar puncture and allow repeated sampling. Changes of plasma phospho-tau-181 in AD are of comparable magnitude and seem to parallel the changes in CSF, may occur in preclinical or predementia stages of the disease, and may differentiate AD from other causes of dementia with adequate accuracy. Plasma phospho-tau-181 may offer a useful alternative to CSF phospho-tau determination, but work still has to be done concerning the optimal method of determination with the highest combination of sensitivity and specificity and cost-effect parameters.
Design and Development of a Multi-Functional Bioinspired Soft Robotic Actuator via Additive Manufacturing
Authors: Kladovasilakis Nikolaos , Sideridis Paschalis , Tzetzis Dimitrios , Piliounis Konstantinos , Kostavelis Ioannis , Tzovaras Dimitrios
Published: August 2022

The industrial revolution 4.0 has led to a burst in the development of robotic automation and platforms to increase productivity in the industrial and health domains. Hence, there is a necessity for the design and production of smart and multi-functional tools, which combine several cutting-edge technologies, including additive manufacturing and smart control systems. In the current article, a novel multi-functional biomimetic soft actuator with a pneumatic motion system was designed and fabricated by combining different additive manufacturing techniques. The developed actuator was bioinspired by the natural kinematics, namely the motion mechanism of worms, and was designed to imitate the movement of a human finger. Furthermore, due to its modular design and the ability to adapt the actuator’s external covers depending on the requested task, this actuator is suitable for a wide range of applications, from soft (i.e., fruit grasping) or industrial grippers to medical exoskeletons for patients with mobility difficulties and neurological disorders. In detail, the motion system operates with two pneumatic chambers bonded to each other and fabricated from silicone rubber compounds molded with additively manufactured dies made of polymers. Moreover, the pneumatic system offers multiple-degrees-of-freedom motion and it is capable of bending in the range of −180° to 180°. The overall pneumatic system is protected by external covers made of 3D printed components whose material could be changed from rigid polymer for industrial applications to thermoplastic elastomer for complete soft robotic applications. In addition, these 3D printed parts control the angular range of the actuator in order to avoid the reaching of extreme configurations. Finally, the bio-robotic actuator is electronically controlled by PID controllers and its real-time position is monitored by a one-axis soft flex sensor which is embedded in the actuator’s configuration.