bluuebunny/crossref_metadata_2025 huggingface dataset:
CXCR6+ tumor-associated macrophages identify immunoevasive colon cancer patients with poor prognosis but favorable response to adjuvant chemotherapy
Authors: Chang Jiang , Lin Songbin , Mao Yihao , Xu Yuqiu , Zhang Zhiyuan , Wu Qi , Chen Yijiao , Zheng Peng , Jiang Yudong , Zhang Zhuojian , Shen Feifan , Wei Ye , Feng Qingyang , Xu Jianmin
Published: June 2022

Background
For high-risk II stage and III stage colon cancer (CC), the side effects and prognostic benefit of adjuvant chemotherapy need to be balanced. It is well known that tumor-associated macrophages (TAMs) play a key role in tumor progression and exist as heterogenous subpopulations. However, the clinical significance of them remains unknown.
Methods
Two independent cohorts of 360 and 126 consecutive colon cancer patients were enrolled from two medical centers of Zhongshan Hospital. The association of CXCR6 + TAMs with clinicalpathological characteristcs, prognosis and response to different duration of adjuvant chemotherapy (ACT) after radical surgery was analyzed. Immunofluorescence and Immunohistochemistry were performed to detect the density of CXCR6 + TAMs and activated CD8 + T cells. The cytokines from peripheral blood were also recorded to explore the immune microenvironment.
Results
The infiltration of CXCR6 + TAMs was higher in tissues, and increased with tumor stage. High density of CXCR6 + TAMs predicted worse overall survival (OS) in all CC patients, and was an independent risk factor verified by univariate and multivariate Cox regression analyses. For high-risk II stage and III stage patients with high density of CXCR6 + TAMs, better disease-free survival (DFS) and OS were observed in 6-month treatment group, especially for those who were male, mucinous, tumor size ≤ 4 cm, without nerve invasion and pMMR. There was a negative relationship between the density of CXCR6 + TAMs and CD8 + T cells, as well as activated CD8 + T cells. Higher level of IL-6, lower level of IL-2R and TNF-α were expressed in high CXCR6 + TAMs patients, which indicated that CXCR6 + TAMs contribute to an immunosuppressive microenvironment.
Conclusion
s CXCR6 + TAMs predicted prognosis and response to different durations of ACT in CC patients. CXCR6 + TAMs were asscoiated an immunosuppressive microenvironment and suppressed the activation of CD8 + T cells.
Mediating Effect of Sleep Disturbance and Rumination on Work-related Burnout of Nurses Treating Patients with Coronavirus Disease
Authors: Zarei Salman , Fooladvand Khadijeh
Published: June 2022

Background: COVID-19 has created significant and unprecedented psychological distress on nurses working with COVID-19 patients. Nurses dealing with such psychological distress are prone to burnout. This study examined the mediating role of sleep disturbance and rumination in the association between psychological distress and work-related burnout of nurses treating COVID-19 patients.Methods: A cross-sectional study was conducted from 26th February to 16th March 2021, on a sample of 250 nurses who were actively working during the COVID-19 pandemic in five referral hospitals in Tehran, Iran. The Oldenburg Burnout Inventory [OLBI], Kessler Psychological Distress Scale [K10], Pittsburgh Sleep Quality Index [PSQI], and Ruminative Responses Scale [RRS] were used to collect data. Data analysis was based on pearson’ correlation analysis and path analysis.Results: Psychological distress has a significant effect on job burnout [p<0.001]. When sleep disturbances were modeled as a mediator, path coefficients of psychological distress showed a significant effect on job burnout [p<0.01]. Also, according to the findings, rumination poses a significant mediating effect on the association between psychological distress and job burnout [p< 0.05].Conclusion: This study demonstrated the importance of designing psychological interventions intended to reduce sleep disturbances and rumination when experiencing stressful events to avoid job burnout among nurses.
The value of local anaesthetic freehand transperineal biopsies in the outpatient setting without antibiotic
Authors: Yang Rui , Jiang Xiao-lu , Yang Kui Ou , Sun Jia-ning , Zhang Feng , Yuan He-jia , Feng Fan , Dou Wei-chao , Wu Ji-Tao , Zhao Hong-wei
Published: June 2022

Background
To evaluate the efficacy of freehand transperineal prostate biopsies (FTPB) under local anaesthetic (LA) in the outpatient setting without antibiotic.
Methods
A total of 218 patients suspicious of prostate cancer (PCa) at the multiparametric magnetic resonance imaging (mpMRI) were analyzed retrospectively from January 2020 to September 2021. All patients were biopsy-naive and had no history of PCa. Suspicious areas on mpMRI were defined and graded using Prostate Imaging Reporting and Data System version 2 (PI-RADS v2). FTPB were performed for each suspicious lesion and followed a 12-core systematic transperineal prostate biopsy (STPB) under LA in the outpatient setting without antibiotic. Histopathological outcomes and complications were recorded. Tolerability was evaluated with a visual analog scale (VAS) assessing discomfort caused by probe, LA injections and biopsies.
Results
PCa was detected in 129 (59.17%) patients and 104 (47.71%) were clinically significant prostate cancer (csPCa). There was exact agreement between FTPB and STPB in 94 (72.88%) patients. FTPB diagnosed 7 cases less PCa than STPB (115 vs 108 cases, P = 0.311) and 19 cases more csPCa than STPB (93 vs 74 cases, P < 0.001). Compared to FTPB alone, the combination of systematic biopsy led to 21 more prostate cancer and 1 more csPCa diagnoses. LA FTPB was well tolerated and no patients developed severe complications (Clavien-Dindo III/IV/V).
Conclusions
FTPB under LA in the outpatient setting without antibiotic was safe, feasible and efficient.
Loneliness in young adulthood: a protocol for a scoping review of the quantitative and qualitative literature.
Authors: Kirwan Emma , O’Súilleabháin Páraic S. , Burns Annette , McMahon Jennifer , Summerville Sarah , Creaven Ann-Marie
Published: June 2022

BackgroundLoneliness refers to the distressing feeling that accompanies the experience of perceiving the quantity or quality of one’s social relationships as inadequate (1). There is increasing recognition of the prevalence of loneliness in young adults. Despite this, there is no existing scoping review on loneliness in young adulthood. Young adults (18-25 years) are in a critical life stage involving diverse social, demographic, biological and cognitive transitions which may affect the development of loneliness. Evidence that loneliness is a risk factor for poorer mental and physical health further emphasises the need to understand the experience in this age group. Therefore, the aim of our scoping review is to provide a summary of the quantitative and qualitative literature on loneliness in young adulthood. MethodsThe proposed scoping review will follow the Joanna Briggs Institute methodology and Arksey and O’Malley’s (2005) framework for scoping reviews. Peer-reviewed journal articles and grey literature in the form of reports or difficult-to-locate studies will be identified by: (a) electronic database searching, (b) contacting national and international researchers in the field, and (c) by posting general requests for relevant information on Twitter. We will include all study designs published in English from 2000 to 2021 where loneliness (defined as subjective) is a key focus of the work and the mean age of participants is ≥ 18 and ≤ 25 years. Editorials, commentaries, opinion pieces, dissertations, and book chapters will be excluded. Articles will be selected for inclusion following screening of titles/abstracts succeeded by full-text screening. Results will be presented in a narrative form to provide a descriptive summary of the literature on loneliness in young adults. DiscussionThe results of this scoping review will provide an up-to-date overview of available research related to loneliness in young adults and will inform our future research in the area. Results will be shared through a peer-reviewed journal publication and conference presentations. RegistrationThis protocol is registered with the Open Science Framework (OSF) available at: https://osf.io/jfcmp.
Faster Grasping of High-Calorie Food Objects in Virtual Reality
Authors: Schroeder Philipp A , Collantoni Enrico , Lohmann Johannes , Butz Martin V. , Plewnia Christian
Published: June 2022

Purpose: Theoretical models and behavioral studies indicate faster approach behavior for high-calorie food (approach bias) among healthy participants. A previous study with Virtual Reality (VR) and online motion-capture quantified this approach bias towards food and non-food cues in a controlled VR environment with hand movements. The aim of this study was to test the specificity of a manual approach bias for high-calorie food in grasp movements compared to low-calorie food and neutral objects of different complexity, namely, simple balls and geometrically more complex office tools.Methods: In a VR setting, healthy participants repeatedly grasped or pushed high-calorie food, low-calorie food, balls and office tools in randomized order with 30 item repetitions. All objects were rated for valence and arousal. Results: High-calorie food was less attractive and more arousing in subjective ratings than low-calorie food and neutral objects. Movement onset was faster for high-calorie food in push-trials, but overall push responses were comparable. In contrast, responses to high-calorie food relative to low-calorie food and to control objects were faster in grasp trials for later stages of interaction (grasp and collect). Non-parametric tests confirmed an approach bias for high-calorie food. Conclusion: A behavioral bias for food was specific to high-calorie food objects. More systematic variations of object fidelity and in clinical populations are outstanding. The utility of VR in assessing approach behavior is confirmed in this study by exploring manual interactions in a controlled environment. Level of evidence: Basic science
Investigation of FDM 3D-Printed PLA Scaffolds for Tissue Engineering
Authors: Eryildiz Meltem
Published: June 2022

Scaffolds with gradient porosity have become very promising candidates for tissue engineering and bone implants because of the combination of better mechanical and biological requirements. In this paper, a novel approach is proposed to design bone scaffolds with gradient porosity similar to the structure of cortical and spongy (cancellous) bones. The radial gradient PLA scaffolds were designed to consist of three different regions with the gyroid infill and, fabricated by Fused deposition modeling (FDM). The scaffolds with radial gradient porosity (G100-40-20) gave highest cell proliferation. Because, mean pore size is an important aspect of scaffolds for mimicking bone.
Research on microstructure and mechanical properties of Al-Zn-Mg-Cu alloy when modified by La, Ce and thermo-mechanical treatment
Authors: Bui Thi Ngoc Mai , Vu Anh Tuan , Nguyen Duong Nam , Tran Duc Huy
Published: June 2022

Influence of rare-earth (La, Ce) and thermo-mechanical treatment on microstructure and mechanical properties of Al-Zn-Mg-Cu alloy are presented in this article. After casting, the alloy which was modified by La, Ce, the grain size of samples obtained around 40–50 µm compared to that of without about 65 µm; and after homogeneous, the grain sizes is about 30 µm. After the cold deformation process, the distance between plates is 10 µm. By EDS after casting, the samples have tended to more La, Ce elements at the grain boundary, after homogeneous, the uniformation distribution of rare-earths was presented by mapping of EDS’s results. In addition, after rolling and heat treatment, the elements were found on the grain boundary and matrix. After recrystallization annealing, the grain size is around 10 µm with the modification sample. The grain size was reduced by two processes of modification as well as thermal-mechanical treatment is a condition for increasing the ductility of the studied alloy. Further, as a result of ability deformation from the tensile test, these results demonstrate that the tensile test obtained 140 % when adding La, Ce contents into the alloy combine with thermal-mechanical treatment. The combined used of La, Ce and thermal-mechanical treatment have increased the ductility of Al-Zn-Mg-Cu alloy
Thermal characterization of straight and curve edge blade liquid fuel swirl burner
Authors: Oyewola Olanrewaju Miracle , Singh Patrick Mark , Akinwonmi Ademola Samuel , Ajide Olusegun Olufemi , Salau Tajudeen Abiola Ogunniyi
Published: June 2022

Accurate monitoring and controlling of the temperature in the combustion chamber can raise the burner efficiency, combustion intensity, fuel consumption and reduce pollutant emission. However, except combustion is accurately monitored and controlled, high concentration of pollutant gases and products like carbon monoxide (CO) and soot can form in the combustion chamber. This paper compares the combustion thermal profiles in a liquid fuel swirl burner using developed straight edge and curve edge blade swirlers at (20, 30, 40, 50 and 60)° for 6, 8, 10 and 12 number of blades in order to optimize the temperature of the burner. Measurements were made in straight and curve blades liquid fuel swirl burner in order to study and compare the thermal characteristics of the straight and curve edge blades in optimizing the combustion dynamics. Similarly, measurements were made for burner without swirl generator and the combustion temperature assessed. Thermal profile was measured in the direction of flow via the six axial ports at distance ((d) =150, 350, 550, 750, 950 and 1150 mm) from the burner exit using Chromium-Zinc thermocouple. Results showed that the wavelength and oscillation of temperature decay in the same type of blade followed the same trend and the peak of combustion intensity is nearer the nozzle for curve edge blades than the straight edge blade. Six (6) blades performed best with the highest temperature in all the ports, while 12 blades gave the least performance. Findings further show that curve edge blade swirlers gave better performance than straight edge blade swirlers with highest temperature of (1065 and 1015) °C, respectively. Hence, it is recommended especially where high temperature and stability application is desirable
Development of a rangefinding method for determining the coordinates of targets by a network of radar stations in counter-battery warfare
Authors: Khudov Hennadii , Zvonko Andrii , Lisohorskyi Bohdan , Solomonenko Yuriy , Mynko Petro , Glukhov Sergey , Irkha Artem , Lishchenko Vitaliy , Mishchenko Yaroslav , Khudov Vladyslav
Published: June 2022

The increase in the accuracy of determining the coordinates of targets is explained by the use of a network of counter-battery radar stations and the rangefinding method for determining the coordinates of targets. The main advantage of using the rangefinding method for determining the coordinates of targets in a network of counter-battery radar stations is to ensure the required accuracy in determining the coordinates of targets without using accurate measurement of angular coordinates. The minimum geometry of the system, which ensures the use of the rangefinding method for determining coordinates, is given. The method of determining the coordinates of targets by a network of counter-battery radar stations has been improved. In contrast to the known ones, information about the range to the target is additionally used in a spatially distributed network of radar stations for counter-battery combat. The boundaries of the working zones of the network of two and three counter-battery radar stations are calculated. The features of creating a continuous strip using the rangefinding method for determining the coordinates of the target are considered. Statistical modeling of the rangefinding method for determining the plane coordinates of the target has been carried out.

It has been established that the use of the rangefinding method ensures the determination of the planar coordinates of the target in a sector of at least 120°. The targets are at a distance of direct radio visibility in relation to the counter-battery radar. The root-mean-square error in determining the target range in this case is no more than 50 m. It has been established that the creation of continuous bands of a low-altitude radar field at a certain height is possible by arranging radar stations in a line. In this case, the distance between the counter-battery radar stations should be no more than half the target detection range at this height
Synthesize and characterization of artificial human bone developed by using nanocomposite
Authors: Dong Pham Van , Patil Santosh R. , Shirguppikar Shailesh S. , Tu Do Ngoc , Phan Nguyen Huu , Thanh Le Thi Phuong , Vu Ngo Ngoc
Published: June 2022

The combination of biopolymers with bioceramics plays vital role in development of artificial bone. Hydroxyapatite is extensively used as a material in prosthetic bone repair and replacement. In this paper synthesis of Hydroxyapatite- Polymethyl methacrylate – Zirconia (Hap-PMMA-ZrO2) composite by using powder metallurgy technique. The mechanical, morphological, In-vitro biocompatibility and tribological properties were characterized by universal testing machine, micro-vickers hardness tester, high resolution transmission electron microscope (HR-TEM), MTT assay and pin-on-disc setup. In-vitro cytotoxicity test on HeLa cell lines shows cell viability constant when doses concentration increases so material found non-toxic. Results show that micro Vickers hardness i.e. 520 approximately matches with natural human bone i.e. 400. Compressive strength is less as compared to human bone because of powder metallurgy route used for fabrication and is 74 MPa. Density of proposed composite artificial human bone i.e. 1.52 g/cc is less as compared to natural bone i.e. 2.90 g/cc. The Hap-PMMA-ZrO2 composite will be good biomaterials for bone repair and replacement work
Propaganda and the war on truth: examining informational inconsistencies governing South Africa’s Covid-19 policy response
Authors: Harmse Demi
Published: June 2022

A review of the intellectual and policy environment announces the absence of transparency and rational discourse in assessing the prevailing Covid-19 policy measures. Contextually, propaganda thrives in times of political uncertainty as it serves to either amplify confusion, induce moral dilemmas,or disguise meanings. To this end, this study examines the quality of political communication, underpinning South Africa’s public policy response to the Covid-19 pandemic. It aims to trace the influence of propaganda in informing policy origins and efficacy as it concerns the lethality of Covid-19. Importantly, informational irregularity must be treated with greater accountability and intellectual inquiry as it concerns masking and vaccine hesitancy. Following a qualitative approach and case study research strategy, this study begins by outlining the propagandistic assault on truth and rationality. Next, it confronts the seeming normality, with which the state, media, intellectual and scientific community have nonchalantly dismissed inconvenient truth in the name of misinformation. Of significance is the war on truth and the growing intellectual appetite for ideological realignment that esteems emotional triumph over empirical soundness. Ultimately, the research shows that scientific rationale has been demoted in favor of social solidarity. Finally, propagandist techniques and elements of deception theory entice the analytical appetite by exposing the modus operandi of deceptive operations at work in both masking and vaccine campaigns. The key findings indicate the use of propaganda and deception tactics at play in perception management with a view of influencing public action, corrupting public discourse and delegitimizing the need for factual accountability, concerning compliance with incoherent Covid-19 policy measures.
STUDI KELAYAKAN DAN MAGANG USAHA PADA BADAN USAHA MILIK DESA (BUMDESA) KUTA SEJAHTERA, KABUPATEN SUMBA TIMUR
Authors: Pakereng Yulita Milla , Hutar Alliny N. R. , Kelen Lusianus H. S. , Radja Melkianus , Humba Yohanis Ndawa R. , Limu Erastus Nggaba K. , Suryani Dewi C. , Kudji Merlan R. R. , Mbakundima Anggreni M.
Published: June 2022

Pengabdian kepada Masyarakat (PkM) yang dilakukan ini adalah berupa studi kelayakan terhadap usaha yang dikelola oleh Badan Usaha Milik Desa (BUMDesa) Kuta Sejahtera yang terletak di Desa Kuta, Kecamatan Kanatang, Kabupaten Sumba Timur, Provinsi Nusa Tenggara Timur, kemudian setelah studi kelayakan dijalankan, kegiatan dilanjutkan dengan magang pada usaha milik BUMDesa tersebut. Kegiatan studi kelayakan dilakukan untuk menilai usaha berdasarkan aspek manajemen berupa: aspek pasar dan pemasaran, teknik dan teknologi, manajemen dan sumber daya manusia, serta aspek keuangan. Sedangkan, magang yang dilakukan pada BUMDesa Kuta Sajehtera dengan memberikan pendampingan terhadap temuan-temuan berupa berbagai kelemahan dari BUMDesa tersebut. Metode yang digunakan adalah studi kelayakan usaha pada BUMDesa kemudian diikuti dengan kegiatan magang. Kegiatan ini dilakukan selama satu semester, yaitu pada semester I Tahun Akademik 2019/2020 yaitu Bulan September 2019 sampai dengan Januari 2020. PkM ini merupakan hasil kolaborasi dosen, khususnya pada program studi Manajemen, mahasiswa dan pengurus BUMDesa Kuta Sejahtera. Hasilnya menunjukkan bahwa berdasarkan studi kelayakan ditemukan empat permasalahan, dari empat permasalah, yang terbagi dalam empat aspek. Kemudian empat masalah difokuskan menjadi tiga bagian karena masalah pada aspek keuangan berada pada luar kendali (faktor eksternal). Setelah itu, tiga kegiatan dilanjutkan berupa magang usaha untuk menyelesaikan masalah.
Predictive modeling of the primary settling tanks based on artificial neural networks for estimating TSS and COD as typical effluent parameters
Authors: Veloz Carlos , Pazmiño-Arias Esteban , Gallardo Andrea M. , Montenegro Jhon , Sommer-Márquez Alicia , Ricaurte Marvin
Published: June 2022

A predictive model based on artificial neural networks (ANNs) for modeling primary settling tanks' (PSTs) behavior in wastewater treatment plants was developed in this study. Two separate ANNs were built using input data, raw wastewater characteristics, and operating conditions. The output data from the ANNs consisted of the total suspended solids (TSS) concentration and chemical oxygen demand (COD) as predictions of PSTs’ typical effluent parameters. Data from a large-scale wastewater treatment plant was used to illustrate the applicability of the predictive model proposal. The ANNs model showed a high prediction accuracy during the training phase. Comparisons with available empirical and statistical models suggested that the ANNs model provides accurate estimations. Also, the ANNs were tested using new experimental data to verify their reproducibility under actual operating conditions. The predicted values were calculated with satisfactory results, having an average absolute deviation of <20%. The model could be adapted to any large-scale wastewater plant to monitor and control the operation of primary settling tanks, taking advantage of the ANNs' learning capacity.
Relación de los mapas semánticos con el incremento de la comprensión lectora
Authors: Cumpa- Valencia Moisés
Published: June 2022

La investigación tuvo como objetivo determinar la relación entre los mapas semánticos y el incremento de la comprensión lectora en un grupo de estudiantes del centro preuniversitario de una universidad de la ciudad de Lima. El diseño empleado es no experimental, descriptivo y correlacional con enfoque cuantitativo. Los instrumentos aplicados fueron una rúbrica para evaluar el diseño eficaz de mapas semánticos y un cuestionario de preguntas de comprensión lectora de elección múltiple. El análisis de consistencia interna de los instrumentos fue determinado por el coeficiente de confiabilidad Alfa de Cronbach con valores de 0.938 y de 0.915, respectivamente, que constituye un nivel alto de fiabilidad. La muestra estuvo conformada por 55 alumnos seleccionados de manera no probabilística, dado que provenían de aulas conformadas por estudiantes de características homogéneas en cuanto a edades y rendimiento académico. Los resultados determinaron que, a mayores niveles en el uso de los mapas semánticos, se obtienen mayores niveles de comprensión lectora. Se concluye, por tanto, promover el empleo de este organizador gráfico en las actividades educativas que requieran mejorar la comprensión lectora
Segmentación de los alumnos ingresantes a una universidad pública aplicando el algoritmo K-prototype
Authors: Chavez Ledvir , Salinas Jesus
Published: June 2022

En la actualidad, el análisis de datos es una labor desafiante, especialmente en el campo de la educación, debido a que se realizan investigaciones profundas para conocer, entender y gestionar la diversidad de alumnos que ingresan a cada institución superior y con ello plantear estrategias educativas para mejorar el modelo de enseñanza – aprendizaje. El objetivo de este artículo fue caracterizar el perfil de los ingresantes de una universidad pública respecto a sus variables sociodemográficas, económicas y de rendimiento académico utilizando el algoritmo K-prototypes, para lo cual se utilizó datos de alumnos ingresados a la Universidad Nacional Agraria La Molina (Lima, Perú) recolectados a partir del examen de admisión, ficha del ingresante y su certificado de estudios escolares. Se pudo determinar que los ingresados en estudio se ajustan a 5 perfiles, cada uno con características propias, permitiendo agrupar a los ingresados con características similares, contribuyendo a la mejora de políticas de acompañamiento, impulsando cambios a favor de la calidad educativa y promoviendo la renovación de los espacios de enseñanza de manera personalizada en torno al perfil del alumno que la universidad gestiona.
Crystallization of the Apo-Forms of the D-Lactate Dehydrogenases from Lactobacillus Pentosum and Leuconostoc Mesenteroides
Authors: Stoll Vincent S. , Kimber Matthew S. , Macfarlane Emma L.  A. , Eikmanns Ulrich , Taguchi Hayao , Pai Emil F.
Published: June 2022

NADH dependent D-lactate dehydrogenases from Lactobacillus pentosum and from Leuconostoc mesenteroides
have been crystallized in their apo-forms by the hanging drop method. The L. pentosum enzyme adopts space
group PI with unit cell dimensions a= 54.5Å, b = 58.2Å, c = 61.2Å, α= 74.4°, β= 83.9° and γ= 82.9°. The
L. mesenteriodes enzyme crystallizes in space group C2 with a unit cell of a= 223.2Å, b = 62.8 Å, c = 143.5Å, β,= 92.4°. The crystals diffract to at least 2.4Å and 3.0Å resolution, respectively, and are stable in the x-ray beam.
Factors and Mechanisms Affecting the Secretion of Recombinant Protein
in CHO Cells
Authors: Wang Tian-Yun , Feng Hui-Gen , Yang Yong-Xiao , Li Qin , Li Wei-Dong
Published: June 2022

The market demand for recombinant therapeutic proteins（RTPs) has promoted the
development of various protein expression host and bioprocessing technologies. Since mammalian
cells have the unique advantage of being able to direct the correct folding of proteins and provide
post-translational processing such as complex glycosylation, the RTPs produced by them currently
account for approximately 80% of the approved marketed RTPs. Among them, Chinese hamster
ovary (CHO) cells are currently the preferred host cells for the production of RTPs. Production of
RTPs in CHO cells involves the synthesis, processing, transport, and secretion of proteins. The
secretion process of proteins is one of the key steps, which greatly limits the yield and quality of
RTPs. Here, we review the recombinant protein secretion process of CHO cells and its influencing
factors, and further discuss the optimization strategy for recombinant protein secretion and expression
in CHO cells.
Emerging Hop Japanese Pollinosis in Asia
Authors: Park Hae-Sim , Jung Chang-Gyu
Published: June 2022

Hop Japanese (Hop J) pollen has been considered one of the major causative pollen allergens
in Far East Asia in the autumn season. The sensitization rate, pollen count, and allergenicity of Hop J
pollens are increasing in this region because of increased air pollution and global warming. However,
commercial skin test agents for the diagnosis and allergen extracts for the allergen immunotherapy are
not available. This review summarizes recent data on clinical impact of Hop J pollinosis in order to facilitate
the development of diagnostic and therapeutic agents for this region.
Caffeic Acid Phenethyl Ester: A Potential Anticancer Bioactive Constituent
of Propolis
Authors: Kapare Harshad , Nagaraj Srishti , Wakalkar Shweta , Rathi Karishma
Published: June 2022

Background:

Currently, synthetic therapeutic drugs used in cancer treatment are targeting
cancerous tissues that also affect noncancerous dividing cells, which exhibit side effects that
decrease the immune response and affect the quality of life. Traditional/ natural products are well
proven for a variety of therapeutically active chemical constituents. Caffeic acid phenethyl ester
(CAPE) is one of the bioactive molecules found in the natural product propolis is now attracting
researcher’s interest because of its anticancer potential.

Objective:

The aim of this review is to summarize and critically analyze the current evidence on the
therapeutic effects of CAPE in various types of cancer cells, both in terms of in vitro and in vivo,
along with supportive anti-inflammatory & antioxidant activity in cancer therapy.

Methods:

This study focused on the mechanism pathways, synergism of CAPE. Various conventional
and advanced targeted nanotechnology based formulation approaches developed for the delivery
of CAPE as a promising therapeutic agent were also discussed in detail in terms of challenges
and future opportunities.

Conclusion:

Overall study summarized and demonstrated the excellent therapeutic potential, mechanisms,
and formulation approaches of CAPE as a therapeutic bioactive molecule. Study and research
further can be initiated for the investigation of biopharmaceutical aspects for therapeutic and
clinical applications.
Could High-intensity Interval Training be Inappropriate in Pulmonary
Rehabilitation of COVID-19 Recovered Patients? Opinion Article
Authors: Ahmad Ahmad Mahdi
Published: June 2022

Pulmonary rehabilitation is a multidisciplinary intervention recommended by international guidelines in the management of COVID-19 patients and/or post-COVID-19 conditions with mid- and long-term complications of the initial illness. Physiotherapy-based pulmonary rehabilitation has been receiving considerable attention since the COVID-19 pandemic, with supervised exercise training being probably the most important component. Current guidelines clearly encourage low- to moderate-intensity exercises to begin with for COVID-19 recovered patients, and discourage high-intensity/vigorous exercises. However, the ongoing interests in high-intensity interval training (HIIT) have extended to post-COVID-19 rehabilitation, based on its efficacy and safety in other patient populations. In this perspective, several reasons behind the possibility of the inappropriateness of the HIIT in post-COVID-19 conditions will be discussed, supported by evidence. In addition, the key points for safe exercise after COVID-19 illness will be listed.
Research on Load Balancing MapReduce Equivalent Join Based on Intelligent
Sampling and Multi Knapsack Algorithm
Authors: Jia Songhao , Yang Cai , Yang Jizheng , Chen Xing , Liu Yan
Published: June 2022

Background:

With the rapid development of science, more data is available to human
beings. Therefore, the storage and calculation of big data have become the focus of scientific research.
MapReduce performs well in the big data processing. However, it is prone to data skew,
which affects the overall efficiency of the data processing cluster.

Objective:

Aiming at the low efficiency of MapReduce data join, this paper proposes an intelligent
data join load balancing algorithm based on dynamic programming. The algorithm introduces
data sampling and partition algorithms. Due to the high performance of dynamic programming
in the data constraint problem, it is used to solve the data skew problem intelligently.

Methods:

Firstly, the causes of data skew are analyzed and the data partition method is improved.
The algorithm introduces a data sampling method. In the task allocation stage, the multidimensional
knapsack algorithm is used. Different key values are evenly divided to each computing
node through the load cost. Finally, The performance of the improved algorithm is verified by
experiments.

Results:

The experimental results show that compared with the traditional load balancing algorithm
and the existing improved algorithm, the new algorithm improves the data processing efficiency,
reduces the data skew problem and better solves the problem of data load imbalance.

Conclusion:

A two-table equivalent join load balancing algorithm based on key cost has been
proposed. The algorithm creatively combines dynamic programming with intelligent data sampling,
which greatly improves the efficiency and quality of data processing. The algorithm is worthy
of popularization and application.
Ab initio исследование NaMFe(MoO-=SUB=-4-=/SUB=-)-=SUB=-3-=/SUB=- (M=Mn, Fe, Co, Ni, Zn): электронная структура, диффузия натрия и потенциалы
Authors: Сердцев А.В. , Медведева Н.И.
Published: June 2022

The electronic structure of NaMFe(MoO4)3 (M = Mn, Fe, Co, Ni, Zn) molybdates isostructural α-NaFe2(MoO4)3 or β-NaFe2(MoO4)3 has been studied for the first time. Taking into account the electronic correlation in the Hubbard model, it is shown that these compounds are semiconductors with a band gap from 1.2 to 2.6 eV. Sodium diffusion pathways in α-NaFe2(MoO4)3 and β-NaFe2(MoO4)3 structures have been established, it is shown that in compounds with the structure of β-NaFe2(MoO4)3 the energy barrier is almost twice lower. Modeling of sodium extraction showed that these molybdates should have a high potential from 3 to 5 V and small changes in the volume and enthalpy of formation depending on the sodium content. According to our results, low-symmetric molybdates NaMFe(MoO4)3 (M = Mn, Fe, Co, Ni, Zn) have diffusion properties and cathode voltage similar to NASICON-structure type compounds, and can also be promising materials for sodium-ion batteries.
Рентгеновские фотоэлектронные спектры молекул кислорода, адсорбированных на нанопленках иттербия
Authors: Митцев М.А. , Кузьмин М.В.
Published: June 2022

The adsorption of oxygen molecules on ytterbium nanofilms of various thicknesses has been studied by X-ray photoelectron spectroscopy and contact potential difference measurements. It has been established that the maximum number of O2 molecules that can be adsorbed on the films increases with the film thickness. Such behavior is observed at thicknesses less than 10 monolayers. This size effect is ultimately due to the fact that an indispensable condition for the adsorption process at high doses of oxygen is the electron transfer from the film to molecules, and that the maximum number of electrons that can participate in this process depends on the nanolayer thickness. Localization of a negative charge on O2 molecules occurring in at high doses of adsorbate, causes a weakening of the bonding of 1s electrons in these molecules. In addition, it should lead to a weakening of the bond between oxygen atoms in O2 molecules.
Ударно-радиационная рекомбинация ионов Ne-=SUP=-++-=/SUP=- и Ne-=SUP=-+-=/SUP=- c электронами в распадающейся плазме барьерного разряда низкого давления
Authors: Иванов В.А.
Published: June 2022

The results of an experimental study and simulation of radiation generated by the processes of collisional-radiative recombination of neon ions Ne+ and Ne++ with electrons of the decaying plasma are presented. Plasma was created by a barrier discharge in a cylindrical glass tube with electrodes on its surface. Experimental conditions: neon pressure 0.65 Torr, electron density at the initial stage of the afterglow [e] ≈ (1 - 5) • 1011 cm-3. The main attention is paid to the comparative analysis of the collisional-radiative recombination of Ne+ and Ne++ ions based on the numerical solution of coupled differential equations for the densities of charged and long-lived excited particles and the electron temperature, taking into account the main elementary processes in decaying neon plasma. Comparison of the model solutions with the intensities of ionic and atomic spectral lines measured by the multichannel photon counting method indicates the need to refine the dependence of the rate of collisional-radiative recombination on the ion charge.
Спинорное представление двойной группы икосаэдра и запутанные состояния волновых функций для фуллерена С-=SUB=-60-=/SUB=-
Authors: Сухарев А.Г.
Published: June 2022

Симметрия группы икосаэдра --- инструмент, позволяющий получить аналитическое описание свойств фуллерена в рамках модели молекулярных орбиталей. Дальнейшее развитие модели фуллерена связано с учетом полуцелой статистики электронных состояний. Двойная группа икосаэдра описывает объекты обеих статистик, однако мы ограничимся ее подмножеством для фермионов --- спинорным представлением группы икосаэдра. В этом представлении удается снять ограничение на четность координатной части волновой функции и построить таблицу характеров для произвольных значений суммарного момента. Симметрия икосаэдра создает алгебраическую структуру кольца вычетов по модулю пять для проекций момента на выбранную ось. Поэтому состояния с проекцией суммарного момента ± 5/2 целиком попадают в одно представление. Заполнение состояний с моментом выше 5/2 приводит к возникновению волновых функций, состоящих из нескольких сцепленных спиноров. Ключевые слова: двойная группа икосаэдра, неприводимые представления, спиноры, спин-орбитальное взаимодействие, запутывание.
Генерация второй гармоники в поверхностном слое диэлектрической сфероидальной частицы. I. Аналитическое решение
Authors: Капшай В.Н. , Шамына А.А. , Толкачёв А.И.
Published: June 2022

The problem of second-harmonic generation by a plane elliptically polarized electromagnetic wave in a thin optically nonlinear surface layer of a dielectric particle shaped as an ellipsoid of revolution is solved. The generalized Rayleigh–Gans–Debye approximation is used for an analytical description with taking into account the difference in refractive indices of the medium corresponding to the frequencies of the exciting and generated radiation. The limiting forms of functions are obtained, with the use of which the electric field strength of the generated radiation is expressed. The order of dependence of these functions on the linear dimensions is found, when the lengths of the semiaxes of the particle are small compared to the wavelength of the exciting radiation and their ratio remains constant. It was found that the power density of the generated radiation in this case is determined to a greater extent by the chiral components of the nonlinear dielectric susceptibility tensor and is proportional to the fourth power of the length of the semiaxis of the particle, if the shape of the spheroidal particle differs significantly from the spherical one. The solution of this problem, obtained by other authors, is supplemented for the possibility of applying to the description of generation in the surface layer of a dielectric particle not only in the form of a prolate, but also in the form of an oblate spheroid. Corrections of inaccuracies and misprints made in similar works by other authors are proposed. The relationships between the formulas used in these works are found, taking into account the corrections and the formulas used in this work.
In-hospital Enrollment Into an Electronic Patient Portal Results in Improved Follow-up After Orthopedic Surgery: Cluster Randomized Controlled Trial
Authors: Bhashyam Abhiram R , Bansal Mira , McGovern Madeline M , van der Vliet Quirine M J , Heng Marilyn
Published: June 2022

Background

Electronic patient portal (EPP) use is associated with lower no-show rates and increased patient satisfaction. However, there are disparities in enrollment into these communication platforms.

Objective

We hypothesized that guided inpatient enrollment into an EPP would improve clinical follow-up and EPP use rates for patients who underwent orthopedic surgery compared to the usual practice of providing information in the discharge summary.

Methods

We performed a randomized controlled trial of 229 adult patients who were admitted to the hospital for an orthopedic condition that required a 3-month follow-up visit. Patients were cluster-randomized by week to either the control or intervention group. The control group received information on how to enroll into and use the EPP in their discharge paperwork, whereas the intervention group was actively enrolled and taught how to use the EPP. At 3 months postdischarge, the patients were followed to see if they attended their follow-up appointment or used the EPP.

Results

Of the 229 patients, 83% (n=190) presented for follow-up at 3 months (control: 93/116, 80.2%; intervention: 97/113, 85.8%; P=.25). The likelihood of EPP use was significantly higher in the intervention group (control: 19/116, 16.4%; intervention: 70/113, 62%; odds ratio [OR] 8.3, 95% CI 4.5-15.5; P<.001). Patients in the intervention group who used the EPP were more likely to present for postsurgical follow-up (OR 3.59, 95% CI 1.28-10.06; P=.02).

Conclusions

The inpatient enrollment of patients who underwent orthopedic surgery into an EPP increased EPP use but did not independently result in enhanced follow-up. Patients who were enrolled as inpatients and subsequently used the portal had the highest likelihood of 3-month follow-up.

Trial Registration

ClinicalTrials.gov NCT03431259; https://clinicaltrials.gov/ct2/show/NCT03431259
Introducing RuminAid:
The Development and Focus Group Testing of a Mobile Health App for Depression (Preprint)
Authors: Rosenfeld Eve Alexandra , Lyman Cassondra , Roberts John E
Published: June 2022

UNSTRUCTURED

Effective treatments for depression exist, yet access to evidence-based care remains limited. Mobile health (mHealth) apps offer one avenue for improving access. We describe the development of RuminAid, a mHealth app that uses evidence-based techniques. This intervention targets a single key depressogenic process—rumination. Its behavioral strategies are rooted in empirically-supported interventions for depression and rumination. RuminAid is consistent with recommendations by mHealth researchers and integrates “gamified” elements. The goal of RuminAid is to teach users to increase awareness of ruminative episodes, deploy behavioral strategies to replace habitual rumination, and address rumination-related deficits in attention. An initial focus-group study found that the consumer-rated quality of a storyboard version of RuminAid was in the acceptable to good range. Participants endorsed overwhelming positive beliefs about the perceived impact of RuminAid. Namely, 95% of participants reported that they believe RuminAid will help depressed ruminators with depression and rumination. Results highlighted a need for improved app aesthetics (eg, a more appealing color scheme and modern design). Subsequent modifications based on focus-group feedback and future directions are discussed.
HEAD AND NECK CARCINOMA STEM CELLS. DIAGNOSTIC, PROGNOSTIC AND THERAPEUTIC TARGETS
Authors: Stankovic Petar , Milisavljević Dušan , Stanković Tamara
Published: June 2022

Head and neck carcinoma arise from the mucosal lining of the upper aerodigestive tract, affecting more than half a million people worldwide each year. Although cancer tissue has many heterogeneous cells with different phenotypes, only a subset of these cancer cells proliferate extensively and have the potential to give rise to all other tumor cells. They are named carcinoma stem cells (CSC). CSCs can persist in tumors and cause relapse and metastasis by producing a new tumor. Head and neck carcinoma stem cells (HNCSC) share a common CD44+ phenotype. What drives a normal stem cell into a malignant stem cell is insufficiently understood. Many regulating pathways were analyzed, such as:Receptor Tyrosine Kinases, Sonic Hedgehog, Notch, Wnt, and Bmi1. Also, the miRNA ratio or epigenetic alteration pattern is potential subject of further studies. This would enable better therapy and survival rate in HNSCC.
CONCENTRATION OF POLYAMINES IN THE RAT LIVER DURING POSTNATAL PERIOD
Authors: Bjelaković Marko , Sokolović Dušan , Bjelaković Milica
Published: June 2022

Polyamines, spermine, spermidine, and putrescine are ubiquitous in living cells. Polyamines play important roles in cell growth, proliferation, and survival. Our aim was to determine the concentration of the spermine, spermidine, and putrescine in the rat liver during the first 6 months of postnatal life. A total of 45 albino Wistar rats, maintained under controlled temperature (20±2°C) in the animal room facilities were included in this study. On the day 1, 3.5 months, and 6 months of postnatal life, rats were sacrificed by cervical dislocation. Thr liver was removed and washed in the 0.9% solution of sodium chloride. Concentrations of spermine, spermidine, and putrescine were determined. Concentration of polyamines in the liver tissue of the Wistar albino rats aged 1 day, 3.5 months, and 6 months was respectively: spermine (Sp) (33.81 ± 3.04; 128.15 ± 6.62; 74.34 ± 1.12 mg/g of wet weight); spermidine (Spd) (121.92 ± 6.23; 53.34 ± 3.31; 56.32± 1.41 mg/g wet weight); and putrescine (Put) (8.92 ± 0.98; 9.37 ± 0.98; 20.93 ± 1.15 mg/g wet weight). Polyamine concentration in the rat liver fluctuated during postnatal life. The concentration of the spermidine was highest in the rat liver on the first postnatal day, much higher than spermine, this ratio inverted in 3.5 months old rats. The concentrations of the spermidine and spermine were almost equal at the 6 months of the postnatal life. The concentration of putrescine steadily increases during postnatal life.
SARS-COV-2 ANTIGEN SCREENING DURING THE FIRST POST-LOCKDOWN MAJOR MUSIC FESTIVAL IN NOVI SAD, SERBIA, IN JULY 2021
Authors: Lazović Maja , Bojat Veselin , Vuković Vladimir , Smiljanić Nina , Milić Marija , Velicki Radmila , Nikolić Nataša , Pustahija Tatjana , Ristić Mioljub
Published: June 2022

Many countries have resumed mass gathering events like music festivals, despite potential risk of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) transmissions. This study aimed to examine the frequency of SARS-CoV-2 infection among visitors to a mass event, the EXIT festival, in relation to the proposed preventive measures. A total of 466 visitors were included in this longitudinal study conducted in Novi Sad, Serbia, during July, 2021. All subjects were tested with RDT-Ag test for the presence of SARS-CoV-2 virus at the beginning of the study and seven days after. Basic socio-demographic and epidemiological data were collected through a questionnaire. The average age of participants was 28.34 ± 8.87 years with the majority of men (53.7%). There were 170 (36.5%) vaccinated participants. A 97.1% in vaccinated and 92.2% in unvaccinated group, reported to regularly wear a protective mask (p=0.029). At the second cross-sectional testing, 354 subjects were tested, of which 150 (42.4%) were vaccinated. There was no statistically significant difference in application of preventive measures during and after the festival, in respect to vaccination status of the participants. Twenty participants (5.6%) reported some of the COVID-19-like symptoms in the first seven days after visiting the festival, but no COVID-19 infection was confirmed at the RDT-Ag testing. Despite the potential effectiveness of applied preventive measures such as RDT-Ag screening, mask-wearing and vaccination, additional caution is needed when holding these events during a period of high SARS-CoV-2 transmission, as well as when the new virus variants emerge in community.