bluuebunny/crossref_metadata_2025 huggingface dataset:
BEX1 and BEX4 Induce GBM Progression through Regulation of Actin Polymerization and Activation of YAP/TAZ Signaling
Authors: Lee Sungmin , Kang Hyunkoo , Shin Eunguk , Jeon Jaewan , Youn HyeSook , Youn BuHyun
Published: September 2021

GBM is a high-grade cancer that originates from glial cells and has a poor prognosis. Although a combination of surgery, radiotherapy, and chemotherapy is prescribed to patients, GBM is highly resistant to therapies, and surviving cells show increased aggressiveness. In this study, we investigated the molecular mechanism underlying GBM progression after radiotherapy by establishing a GBM orthotopic xenograft mouse model. Based on transcriptomic analysis, we found that the expression of BEX1 and BEX4 was upregulated in GBM cells surviving radiotherapy. We also found that upregulated expression of BEX1 and BEX4 was involved in the formation of the filamentous cytoskeleton and altered mechanotransduction, which resulted in the activation of the YAP/TAZ signaling pathway. BEX1- and BEX4-mediated YAP/TAZ activation enhanced the tumor formation, growth, and radioresistance of GBM cells. Additionally, latrunculin B inhibited GBM progression after radiotherapy by suppressing actin polymerization in an orthotopic xenograft mouse model. Taken together, we suggest the involvement of cytoskeleton formation in radiation-induced GBM progression and latrunculin B as a GBM radiosensitizer.
Effects of Prenatal Phthalate Exposure and Childhood Exercise on Maternal Behaviors in Female Rats at Postpartum: A Role of Oxtr Methylation in the Hypothalamus
Authors: Lee Yi-Ju , Lin Hwai-Ting , Chaudhary Muhammad Asad , Lee Yi-Ching , Wang Dean-Chuan
Published: September 2021

Both the detrimental effect of prenatal exposure to di-(2-ethylhexyl)-phthalate (DEHP) and the beneficial effects of physical exercise on brain functions have been reported. The oxytocin pathway has been implicated in the onset of maternal behaviors. Epigenetic modification of the oxytocin receptor gene (OXTR) through DNA methylation has been associated with the pathogenesis of neuropsychiatric disorders. The purpose of this study was to investigate the effects of prenatal DEHP exposure on oxytocin-regulated maternal behaviors and to examine the protective effect of exercise. Pregnant rats (F0) were fed with vehicle or DEHP during gestation and the offspring females (F1) were assessed for their maternal behaviors by pup retrieval test at postpartum. The results showed that reduced pup retrieval activities without significant alteration of stress responses were observed in the prenatally DEHP-exposed females. Prenatal DEHP exposure decreased the expressions of oxytocin, Oxtr mRNA, and oxytocin receptor, and increased Oxtr methylation in the hypothalamus of postpartum female rats. There were no significant effects of exercise on behavioral, biochemical, and epigenetic measurements. These results suggest that prenatal DEHP exposure has a long-term adverse effect on maternal behaviors; Oxtr hyper-methylation may be a potential epigenetic mechanism for this alteration, which cannot be prevented by physical exercise during childhood.
The pH-Dependent Controlled Release of Encapsulated Vitamin B1 from Liposomal Nanocarrier
Authors: Juhász Ádám , Ungor Ditta , Várkonyi Egon Z. , Varga Norbert , Csapó Edit
Published: September 2021

In this work, we firstly presented a simple encapsulation method to prepare thiamine hydrochloride (vitamin B1)-loaded asolectin-based liposomes with average hydrodynamic diameter of ca. 225 and 245 nm under physiological and acidic conditions, respectively. In addition to the optimization of the sonication and magnetic stirring times used for size regulation, the effect of the concentrations of both asolectin carrier and initial vitamin B1 on the entrapment efficiency (EE %) was also investigated. Thermoanalytical measurements clearly demonstrated that after the successful encapsulation, only weak interactions were discovered between the carriers and the drug molecules. Moreover, the dissolution profiles under physiological (pH = 7.40) and gastric conditions (pH = 1.50) were also registered and the release profiles of our liposomal B1 system were compared with the dissolution profile of the pure drug solution and a manufactured tablet containing thiamin hydrochloride as active ingredient. The release curves were evaluated by nonlinear fitting of six different kinetic models. The best goodness of fit, where the correlation coefficients in the case of all three systems were larger than 0.98, was reached by application of the well-known second-order kinetic model. Based on the evaluation, it was estimated that our liposomal nanocarrier system shows 4.5-fold and 1.5-fold larger drug retention compared to the unpackaged vitamin B1 under physiological conditions and in artificial gastric juice, respectively.
One-Step Surface Immobilization of Protein A on Hydrogel Nanofibers by Core-Shell Electrospinning for Capturing Antibodies
Authors: Naganuma Chihiro , Moriyama Kosuke , Suye Shin-ichiro , Fujita Satoshi
Published: September 2021

Nanofibers (NFs) are potential candidates as filter materials for affinity separation owing to their high liquid permeability based on their high porosity. Multiple and complex processes were conventionally performed to immobilize proteins for modifying NF surfaces. A simple method must be developed to immobilize proteins without impairing their biological activity. Herein, we succeeded in fabricating NFs with a core of cellulose acetate and a shell of hydrophilic polyvinyl alcohol immobilized with staphylococcal recombinant protein A by a one-step process based on core-shell electrospinning. A total of 12.9 mg/cm3 of antibody was captured in the fiber shell through high affinity with protein A immobilized in an aqueous environment of the hydrogel. The maximum adsorption site and dissociation constant evaluated by the Langmuir model were 87.8 µg and 1.37 µmol/L, respectively. The fiber sheet withstood triplicate use. Thus, our NF exhibited high potential as a material for membrane chromatography.
Copper Dithiocarbamates: Coordination Chemistry and Applications in Materials Science, Biosciences and Beyond
Authors: Hogarth Graeme , Onwudiwe Damian C.
Published: September 2021

Copper dithiocarbamate complexes have been known for ca. 120 years and find relevance in biology and medicine, especially as anticancer agents and applications in materials science as a single-source precursor (SSPs) to nanoscale copper sulfides. Dithiocarbamates support Cu(I), Cu(II) and Cu(III) and show a rich and diverse coordination chemistry. Homoleptic [Cu(S2CNR2)2] are most common, being known for hundreds of substituents. All contain a Cu(II) centre, being either monomeric (distorted square planar) or dimeric (distorted trigonal bipyramidal) in the solid state, the latter being held together by intermolecular C···S interactions. Their d9 electronic configuration renders them paramagnetic and thus readily detected by electron paramagnetic resonance (EPR) spectroscopy. Reaction with a range of oxidants affords d8 Cu(III) complexes, [Cu(S2CNR2)2][X], in which copper remains in a square-planar geometry, but Cu–S bonds shorten by ca. 0.1 Å. These show a wide range of different structural motifs in the solid-state, varying with changes in anion and dithiocarbamate substituents. Cu(I) complexes, [Cu(S2CNR2)2]−, are (briefly) accessible in an electrochemical cell, and the only stable example is recently reported [Cu(S2CNH2)2][NH4]·H2O. Others readily lose a dithiocarbamate and the d10 centres can either be trapped with other coordinating ligands, especially phosphines, or form clusters with tetrahedral [Cu(μ3-S2CNR2)]4 being most common. Over the past decade, a wide range of Cu(I) dithiocarbamate clusters have been prepared and structurally characterised with nuclearities of 3–28, especially exciting being those with interstitial hydride and/or acetylide co-ligands. A range of mixed-valence Cu(I)–Cu(II) and Cu(II)–Cu(III) complexes are known, many of which show novel physical properties, and one Cu(I)–Cu(II)–Cu(III) species has been reported. Copper dithiocarbamates have been widely used as SSPs to nanoscale copper sulfides, allowing control over the phase, particle size and morphology of nanomaterials, and thus giving access to materials with tuneable physical properties. The identification of copper in a range of neurological diseases and the use of disulfiram as a drug for over 50 years makes understanding of the biological formation and action of [Cu(S2CNEt2)2] especially important. Furthermore, the finding that it and related Cu(II) dithiocarbamates are active anticancer agents has pushed them to the fore in studies of metal-based biomedicines.
Chromosomal and Molecular Diversity in the Simulium ornatum Group (Diptera: Simuliidae) in the Western Tian Shan Range of Central Asia
Authors: Adler Peter H. , Werner Doreen , Kampen Helge
Published: September 2021

By any measure, such as abundance, species diversity or geographic range, the Simulium ornatum species group is one of the most successful Palearctic taxa of black flies. To explore potential diversity in this group in the Tian Shan range of Central Asia, we focused on Kyrgyzstan, in which three nominal morphospecies have been recorded. Among our samples, we morphologically identified S. mesasiaticum Rubtsov and a second possible species tentatively identified as S. ferganicum Rubtsov. By analyzing banding patterns of the larval polytene chromosomes, we discovered two fixed inversions, two sex-linked rearrangements, and 19 autosomal rearrangements, including supernumerary B chromosomes. The chromosomal data indicate minimal diversity of only one or two species across the surveyed area of nearly 50,000 km2. Mitochondrial DNA (CO1) sequences fell into three distinct clusters, possibly representing separate species. The chromosomal, molecular, and morphological data indicate that Kyrgyz populations are unique within the S. ornatum group, but the data sets are not entirely congruent. Thus, reconciling data sets and assigning existing names is tentative. Simulium mesasiaticum is linked with undifferentiated sex chromosomes, one of the three CO1 clades, and higher elevations, whereas S. ferganicum is tenuously associated with differentiated sex chromosomes, a separate CO1 clade, and lower elevations. These associations leave one Kyrgyz larva, which is in a third CO1 clade, unlinked to a formal species name. Our analyses also indicate that S. ornatum Meigen sensu stricto, contrary to previous reports, does not occur in Kyrgyzstan and should be deleted from the country’s faunal list.
Comparative Mitogenomic Analysis of Two Longhorn Beetles (Coleoptera: Cerambycidae: Lamiinae) with Preliminary Investigation into Phylogenetic Relationships of Tribes of Lamiinae
Authors: Ren Yifang , Lu Huanhuan , Chen Longyan , Sabatelli Simone , Wang Chaojie , Xie Guanglin , Wang Ping , Liu Meike , Wang Wenkai , Audisio Paolo
Published: September 2021

The subfamily Lamiinae is the most taxonomically diverse subfamily of Cerambycidae, but relationships between tribes of Lamiinae are still unresolved. In order to study the characteristics of the mitogenomes of Lamiinae and the tribal-level phylogenetic relationships, we sequenced the mitogenomes of two species representing two tribes, Agapanthia amurensis (Agapanthiini) and Moechotypa diphysis (Ceroplesini), with a total length of 15,512 bp and 15,493 bp, respectively. The gene arrangements of these two new mitogenomes were consistent with the inferred ancestral insect mitogenomes. Each species contained 37 typical mitochondrial genes and a control region (A + T-rich region), including 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), and two ribosomal RNA genes (rRNAs). All PCGs initiated with the standard start codon ATN, and terminated with the complete stop codons of TAA and TAG, or incomplete stop codon T. All tRNAs could be folded into a clover-leaf secondary structure except for trnS1, in which the dihydrouridine (DHU) arm was reduced. Moreover, we studied the phylogenetic relationships between some tribes of Lamiinae based in mitochondrial PCGs in nucleotides; our results show that the relationships were as follows: (Onciderini + ((Apomecynini + Acanthocinini) + ((Ceroplesini + Agapanthiini) + ((Mesosini + Pteropliini) + ((Dorcaschematini + (Saperdini 1 + (Phytoeciini + Saperdini 2))) + (Batocerini + Lamiini)))))).
Respiratory Nasal Mucosa in Chronic Rhinosinusitis with Nasal Polyps versus COVID-19: Histopathology, Electron Microscopy Analysis and Assessing of Tissue Interleukin-33
Authors: Jeican Ionuț , Gheban Dan , Barbu-Tudoran Lucian , Inișca Patricia , Albu Camelia , Ilieș Maria , Albu Silviu , Vică Mihaela , Matei Horea , Tripon Septimiu , Lazăr Mihaela , Aluaș Maria , Siserman Costel , Muntean Monica , Trombitas Veronica , Iuga Cristina , Opincariu Iulian , Junie Lia
Published: September 2021

(1) Background: Chronic rhinosinusitis with nasal polyps (CRSwNP) is one of the most studied rhinological disorders. Modifications of the respiratory nasal mucosa in COVID-19 patients are so far unknown. This paper presents a comparative morphological characterization of the respiratory nasal mucosa in CRSwNP versus COVID-19 and tissue interleukin (IL)-33 concentration. (2) Methods: We analyzed CRSwNP and COVID-19 samples through histopathology, scanning and transmission electron microscopy and performed proteomic determination of IL-33. (3) Results: Histopathologically, stromal edema (p < 0.0001) and basal membrane thickening (p = 0.0768) were found more frequently in CRSwNP than in COVID-19. Inflammatory infiltrate was mainly eosinophil-dominant in CRSwNP and lymphocyte-dominant in COVID-19 (p = 0.3666). A viral cytopathic effect was identified in COVID-19. Scanning electron microscopy detected biofilms only in CRSwNP, while most COVID-19 samples showed microbial aggregates (p = 0.0148) and immune cells (p = 0.1452). Transmission electron microscopy of CRSwNP samples identified biofilms, mucous cell hyperplasia (p = 0.0011), eosinophils, fibrocytes, mastocytes, and collagen fibers. Extracellular suggestive structures for SARS-CoV-2 and multiple Golgi apparatus in epithelial cells were detected in COVID-19 samples. The tissue IL-33 concentration in CRSwNP (210.0 pg/7 μg total protein) was higher than in COVID-19 (52.77 pg/7 μg total protein) (p < 0.0001), also suggesting a different inflammatory pattern. (4) Conclusions: The inflammatory pattern is different in each of these disorders. Results suggested the presence of nasal dysbiosis in both conditions, which could be a determining factor in CRSwNP and a secondary factor in COVID-19.
New Perspectives on Zirconia Composites as Biomaterials
Authors: Magnani Giuseppe , Fabbri Paride , Leoni Enrico , Salernitano Elena , Mazzanti Francesca
Published: September 2021

Zirconia–alumina composites couple the high toughness of zirconia with the peculiar properties of alumina, i.e., hardness, wear, and chemical resistance, so they are considered promising materials for orthopedic and dental implants. The design of high performance zirconia composites needs to consider different aspects, such as the type and amount of stabilizer and the sintering process, that affect the mechanics of toughening and, hence, the mechanical properties. In this study, several stabilizers (Y2O3, CuO, Ta2O5, and CeO2) were tested together with different sintering processes to analyze the in situ toughening mechanism induced by the tetragonal–monoclinic (t–m) transformation of zirconia. One of the most important outcomes is the comprehension of the opposite effect played by the grain size and the tetragonality of the zirconia lattice on mechanical properties, such as fracture toughness and bending strength. These results allow for the design of materials with customized properties and open new perspectives for the development of high-performance zirconia composites for orthopedic implants with high hydrothermal resistance. Moreover, a near-net shape forming process based on the additive manufacturing technology of digital light processing (DLP) was also studied to produce ceramic dental implants with a new type of resin–ceramic powder mixture. This represents a new frontier in the development of zirconia composites thanks to the possibility to obtain a customized component with limited consumption of material and reduced machining costs.
Conducting-Polymer Nanocomposites as Synergistic Supports That Accelerate Electro-Catalysis: PEDOT/Nano Co3O4/rGO as a Photo Catalyst of Oxygen Production from Water
Authors: Alsultan Mohammed , Ameen Anwer M. , Al-keisy Amar , Swiegers Gerhard F.
Published: September 2021

This work describes how conducting polymer nanocomposites can be employed as synergistic supports that significantly accelerate the rate of electro-catalysis. The nanocomposite PEDOT/nano-Co3O4/rGO is discussed as an example in this respect, which is specific for photo electro-catalytic oxygen (O2) generation from water using light (PEDOT = poly (3,4-ethylenedioxythiophene); rGO = reduced graphene oxide). We show that the conducting polymer PEDOT and the conductive additive rGO may be used to notably amplify the rate of O2-generation from water by the nano catalyst, Co3O4. A composite film containing the precise molar ratio 7.18 (C; PEDOT):1 (Co):5.18 (C; rGO) exhibited high photocatalytic activity (pH 12) for the oxygen evolution reaction (OER) at 0.80 V (vs. Ag/AgCl), with a current density of 1000 ± 50 μA/cm2 (including a photocurrent of 500 μA/cm2), achieved after >42 h of operation under illumination with a light of intensity 0.25 sun. By comparison, the best industrial catalyst, Pt, yielded a much lower 150 μA/cm2 under the same conditions. Oxygen gas was the sole product of the reaction.
The Long-Acting Echinocandin, Rezafungin, Prevents Pneumocystis Pneumonia and Eliminates Pneumocystis from the Lungs in Prophylaxis and Murine Treatment Models
Authors: Cushion Melanie T. , Ashbaugh Alan
Published: September 2021

Rezafungin is a novel echinocandin in Phase 3 development for prevention of invasive fungal disease caused by Candida spp., Aspergillus spp. and Pneumocystis jirovecii in blood and marrow transplantation patients. For such patients, standard antifungal prophylaxis currently comprises an azole for Candida and Aspergillus plus trimethoprim-sulfamethoxazole (TMP-SMX) for Pneumocystis pneumonia (PCP) despite drug-drug-interactions and intolerability that may limit their use, thus, alternatives are desirable. Rezafungin demonstrates a favorable safety profile and pharmacokinetic properties that allow for once-weekly dosing in addition, to antifungal activity against these predominant pathogens. Herein, the in vivo effects of rezafungin against Pneumocystis murina pneumonia were evaluated in immunosuppressed mouse models of prophylaxis and treatment using microscopy and qPCR assessments. In the prophylaxis model, immunosuppressed mice inoculated with P. murina were administered TMP-SMX (50/250 mg/kg 1×/week or 3×/week), caspofungin (5 mg/kg 3×/week), rezafungin (20 mg/kg, 1×/week or 3×/week; 5 mg/kg, 3×/week) intraperitoneally for 2, 4, 6 and 8 weeks, then immunosuppressed for an additional 6 weeks. Rezafungin administered for 4 weeks prevented P. murina from developing infection after rezafungin was discontinued. In the treatment model, immunosuppressed mice with P. murina pneumonia were treated with rezafungin 20 mg/kg 3×/week intraperitoneally for 2, 4, 6 and 8 weeks. Treatment with rezafungin for 8 weeks resulted in elimination of P. murina. Collectively, these studies showed that rezafungin could both prevent infection and eliminate P. murina from the lungs of mice. These findings support the obligate role of sexual reproduction for survival and growth of Pneumocystis spp. and warrant further investigation for treatment of P. jirovecii pneumonia in humans.
Reassessing the Role of Brain Tumor Biopsy in the Era of Advanced Surgical, Molecular, and Imaging Techniques—A Single-Center Experience with Long-Term Follow-Up
Authors: Di Bonaventura Rina , Montano Nicola , Giordano Martina , Gessi Marco , Gaudino Simona , Izzo Alessandro , Mattogno Pier Paolo , Stumpo Vittorio , Caccavella Valerio Maria , Giordano Carolina , Lauretti Liverana , Colosimo Cesare , D’Alessandris Quintino Giorgio , Pallini Roberto , Olivi Alessandro
Published: September 2021

Brain biopsy is the gold standard in order to establish the diagnosis of unresectable brain tumors. Few studies have investigated the long-term outcomes of biopsy patients. The aim of this single-institution-based study was to assess the concordance between radiological and histopathological diagnoses, and the long-term patient outcome. Ninety-three patients who underwent brain biopsy in the last 5 years were analyzed. We included patients treated with stereotactically guided needle, open, and neuroendoscopic biopsies. Most patients (86%) received needle biopsy. Gliomas and primary brain lymphomas comprised 88.2% of cases. The diagnostic yield was 95.7%. Serious complication and death rates were 3.2% and 2.1%, respectively. The concordance rate between radiological and histological diagnoses was 93%. Notably, the positive predictive value of radiological diagnosis of lymphoma was 100%. Biopsy allowed specific treatment in 72% of cases. Disease-related neurological worsening was the main reason that precluded adjuvant treatment. Adjuvant treatment, in turn, was the strongest prognostic factor, since the median overall survival was 11 months with vs. 2 months without treatment (p = 0.0002). Finally, advanced molecular evaluations can be obtained on glioma biopsy specimens to provide integrated diagnoses and individually tailored treatments. We conclude that, despite the huge advances in imaging techniques, biopsy is required when an adjuvant treatment is recommended, particularly in gliomas.
The Impact of Soil-Improving Cropping Practices on Erosion Rates: A Stakeholder-Oriented Field Experiment Assessment
Authors: Tsanis Ioannis K. , Seiradakis Konstantinos D. , Sarchani Sofia , Panagea Ioanna S. , Alexakis Dimitrios D. , Koutroulis Aristeidis G.
Published: September 2021

The risk of erosion is particularly high in Mediterranean areas, especially in areas that are subject to a not so effective agricultural management–or with some omissions–, land abandonment or wildfires. Soils on Crete are under imminent threat of desertification, characterized by loss of vegetation, water erosion, and subsequently, loss of soil. Several large-scale studies have estimated average soil erosion on the island between 6 and 8 Mg/ha/year, but more localized investigations assess soil losses one order of magnitude higher. An experiment initiated in 2017, under the framework of the SoilCare H2020 EU project, aimed to evaluate the effect of different management practices on the soil erosion. The experiment was set up in control versus treatment experimental design including different sets of treatments, targeting the most important cultivations on Crete (olive orchards, vineyards, fruit orchards). The minimum-to-no tillage practice was adopted as an erosion mitigation practice for the olive orchard study site, while for the vineyard site, the cover crop practice was used. For the fruit orchard field, the crop-type change procedure (orange to avocado) was used. The experiment demonstrated that soil-improving cropping techniques have an important impact on soil erosion, and as a result, on soil water conservation that is of primary importance, especially for the Mediterranean dry regions. The demonstration of the findings is of practical use to most stakeholders, especially those that live and work with the local land.
Re-Thinking Peer Reviewing in the Virtual Context: The Roles of Giving and Receiving Online Feedback in L2 Spanish Classrooms
Authors: Illana-Mahiques Emilia
Published: September 2021

This study explores learners’ online peer review practices during a four-week second language writing project. The project was developed in a multi-section Spanish writing course at the college level. The study investigates how college Spanish learners give online feedback to their peers, whether there is any relationship between the feedback roles they assume and their final performance, and the additional factors that may influence online peer reviewing practices. A total of 76 students participated in the study, all of whom received training prior to writing three drafts and giving and receiving feedback comments during two online peer review sessions. Descriptive statistical measures were used to analyze the types of online feedback students used most frequently. The comparative effects of giving comments were analyzed along with those of receiving comments by means of multiple regression analyses, in order to examine the relationship of these elements to final project performance as writers. Results support the learning-by-reviewing hypothesis, which argues that giving feedback to peers helps feedback-givers write better essays themselves. A follow-up analysis also shows that learning by reviewing online is most evident when giving specific types of online feedback, which students of all proficiency levels can learn how to do.
Stress Impact of the Annealing Procedure of Cu-Filled TSV Packaging on the Performance of Nano-Scaled MOSFETs Evaluated by an Analytical Solution and FEA-Based Submodeling Technique
Authors: Huang Pei-Chen , Lee Chang-Chun
Published: September 2021

Stress-induced performance change in electron packaging architecture is a major concern when the keep-out zone (KOZ) and corresponding integration density of interconnect systems and transistor devices are considered. In this study, a finite element analysis (FEA)-based submodeling approach is demonstrated to analyze the stress-affected zone of through-silicon via (TSV) and its influences on a planar metal oxide semiconductor field transistor (MOSFET) device. The feasibility of the widely adopted analytical solution for TSV stress-affected zone estimation, Lamé radial stress solution, is investigated and compared with the FEA-based submodeling approach. Analytic results reveal that the Lamé stress solution overestimates the TSV-induced stress in the concerned device by over 50%, and the difference in the estimated results of device performance between Lamé stress solution and FEA simulation can reach 22%. Moreover, a silicon–germanium-based lattice mismatch stressor is designed in a silicon p-type MOSFET, and its effects are analyzed and compared with those of TSV residual stress. The S/D stressor dominates the stress status of the device channel. The demonstrated FEA-based submodeling approach is effective in analyzing the stress impact from packaging and device-level components and estimating the KOZ issue in advanced electronic packaging.
Microstructure and Properties of Mg-Al-Ca-Mn Alloy with High Ca/Al Ratio Fabricated by Hot Extrusion
Authors: Chu Aimin , Zhao Yuping , ud-din Rafi , Hu Hairong , Zhi Qian , Wang Zerui
Published: September 2021

Mg-Al-Ca-Mn alloys with Ca/Al ≥ 1 of AX33, AX44, and AX55 were prepared by combining three processes of water-cooling semi-continuous cast, homogenization heat treatment, and hot extrusion. The as-fabricated alloys translated into composites consisting of α-Mg solid solution + granular Al2Ca. These alloys exhibited some favourable properties such as a tensile strength of 324~350 MPa at room temperature and 187~210 MPa at elevated temperature of 423 K, an ignition temperature of 1292~1344 K, and so on. Variation trend between performance and content of Al and Ca is given in this paper. The result indicated that the emerged second-phase Al2Ca in the alloys was beneficial to the improvement in mechanical properties, heat resistance, flame retardation, and corrosion resistance.
Phononic Bandgap Optimization in Sandwich Panels Using Cellular Truss Cores
Authors: Quinteros Leonel , Meruane Viviana , Lenz Cardoso Eduardo , Ruiz Rafael O.
Published: September 2021

The development of custom cellular materials has been driven by recent advances in additive manufacturing and structural topological optimization. These contemporary materials with complex topologies have better structural efficiency than traditional materials. Particularly, truss-like cellular structures exhibit considerable potential for application in lightweight structures owing to their excellent strength-to-mass ratio. Along with being light, these materials can exhibit unprecedented vibration properties, such as the phononic bandgap, which prohibits the propagation of mechanical waves over certain frequency ranges. Consequently, they have been extensively investigated over the last few years, being the cores for sandwich panels among the most important potential applications of lattice-based cellular structures. This study aims to develop a methodology for optimizing the topology of sandwich panels using cellular truss cores for bandgap maximization. In particular, a methodology is developed for designing lightweight composite panels with vibration absorption properties, which would bring significant benefits in applications such as satellites, spacecraft, aircraft, ships, automobiles, etc. The phononic bandgap of a periodic sandwich structure with a square core topology is maximized by varying the material and the geometrical properties of the core under different configurations. The proposed optimization methodology considers smooth approximations of the objective function to avoid non-differentiability problems and implements an optimization approach based on the globally convergent method of moving asymptotes. The results show that it is feasible to design a sandwich panel using a cellular core with large phononic bandgaps.
Base Materials’ Influence on Fracture Resistance of Molars with MOD Cavities
Authors: Ciavoi Gabriela , Mărgărit Ruxandra , Todor Liana , Bodnar Dana , Dina Magdalena Natalia , Tărlungeanu Daniela Ioana , Cojocaru Denisa , Farcaşiu Cătălina , Andrei Oana Cella
Published: September 2021

The aim of this study was to compare fracture resistance of teeth presenting medium-sized mesial-occlusal-distal (MOD) cavities using different base materials. Thirty-six extracted molars were immersed for 48 h in saline solution (0.1% thymol at 4 °C) and divided into six groups. In group A, the molars were untouched, and in group B, cavities were prepared, but not filled. In group C, we used zinc polycarboxylate cement, in group D—conventional glass ionomer cement, in group E—resin modified glass ionomer cement, and in group F—flow composite. Fracture resistance was tested using a universal loading machine (Lloyd Instruments) with a maximum force of 5 kN and a crosshead speed of 1.0 mm/min; we used NEXYGEN Data Analysis Software and ANOVA Method (p < 0.05). The smallest load that determined the sample failure was 2780 N for Group A, 865 N for Group B, 1210 N for Group C, 1340 N for Group D, 1630 N for Group E and 1742 N for Group F. The highest loads were 3050 N (A), 1040 N (B), 1430 N (C), 1500 N (D), 1790 N (E), and 3320 N (F), the mean values being 2902 ± 114 N (A), 972 ± 65 N (B), 1339 ± 84 N (C), 1415 ± 67 N (D), 1712 ± 62 N (E), and 2334 ± 662 N (F). A p = 0.000195 shows a statistically significant difference between groups C, D, E and F. For medium sized mesial-occlusal-distal (MOD) cavities, the best base material regarding fracture resistance was flow composite, followed by glass ionomer modified with resin, conventional glass ionomer cement and zinc polycarboxylate cement. It can be concluded that light-cured base materials are a better option for the analyzed use case, one of the possible reasons being their compatibility with the final restoration material, also light-cured.
Effect of 1.5 wt% Copper Addition and Various Contents of Silicon on Mechanical Properties of 1.7102 Medium Carbon Steel
Authors: Salvetr Pavel , Gokhman Aleksandr , Nový Zbyšek , Motyčka Petr , Kotous Jakub
Published: September 2021

Requirements for mechanical properties of steels are constantly increasing, and the combination of quenching and tempering is the method generally chosen for achieving high strength in medium carbon steels. This study examines the influence of various silicon contents from 1.06 to 2.49 wt% and the addition of copper (1.47 wt%) on the behavior of 1.7102 steel starting with the as-quenched state and ending with the tempered condition at the temperature of 500 °C. The microstructure was characterized by SEM and TEM, the phase composition and dislocation density were studied by XRD analysis, and mechanical properties were assessed by tensile and hardness testing, whereas tempered martensite embrittlement was assessed using Charpy impact test and the activation energy of carbide precipitation was determined by dilatometry. The benefit of copper consists in the improvement of reduction of area by tempering between 150 and 300 °C. The increase in strength due to copper precipitation occurs upon tempering at 500 °C, where strength is generally low due to a drop in dislocation density and changes in microstructure. The increasing content of silicon raises strength and dislocation density in steels, but the plastic properties of steel are limited. It was found that the silicon content of 1.5 wt% is optimum for the materials under study.
Low-Coordinate Dinuclear Dysprosium(III) Single Molecule Magnets Utilizing LiCl as Bridging Moieties and Tris(amido)amine as Blocking Ligands
Authors: Brzozowska Maria , Handzlik Gabriela , Zychowicz Mikolaj , Pinkowicz Dawid
Published: September 2021

A low-coordinate dinuclear dysprosium complex {[Dy(N3N)(THF)][LiCl(THF)]}2 (Dy2) with a double bridging ‘LiCl’ moiety and tris(amido)amine (N3N)3− anions as a blocking ligand is synthesized and characterized structurally and magnetically. Thanks to the use of the chelating blocking ligand (N3N)3− equipped with large steric –SiMe3 groups, the coordination sphere of both DyIII ions is restricted to only six donor atoms. The three amido nitrogen atoms determine the orientation of the easy magnetization axes of both DyIII centers. Consequently, Dy2 shows slow magnetic relaxation typical for single molecule magnets (SMMs). However, the effective energy barrier for magnetization reversal determined from the AC magnetic susceptibility measurements is much lower than the separation between the ground and the first excited Kramers doublet based on the CASSCF ab initio calculations. In order to better understand the possible influence of the anticipated intramolecular magnetic interactions in this dinuclear molecule, its GdIII-analog {[Gd(N3N)(THF)][LiCl(THF)]}2 (Gd2) is also synthesized and studied magnetically. Detailed magnetic measurements reveal very weak antiferromagnetic interactions in Gd2. This in turn suggests similar antiferromagnetic interactions in Dy2, which might be responsible for its peculiar SMM behavior and the absence of the magnetic hysteresis loop.
Improved Manta Ray Foraging Optimization for Parameters Identification of Magnetorheological Dampers
Authors: Liao Yingying , Zhao Weiguo , Wang Liying
Published: September 2021

Magnetorheological (MR) dampers play a crucial role in various engineering systems, and how to identify the control parameters of MR damper models without any prior knowledge has become a burning problem. In this study, to identify the control parameters of MR damper models more accurately, an improved manta ray foraging optimization (IMRFO) is proposed. The new algorithm designs a searching control factor according to a weak exploration ability of MRFO, which can effectively increase the global exploration of the algorithm. To prevent the premature convergence of the local optima, an adaptive weight coefficient based on the Levy flight is designed. Moreover, by introducing the Morlet wavelet mutation strategy to the algorithm, the mutation space is adaptively adjusted to enhance the ability of the algorithm to step out of stagnation and the convergence rate. The performance of the IMRFO is evaluated on two sets of benchmark functions and the results confirm the competitiveness of the proposed algorithm. Additionally, the IMRFO is applied in identifying the control parameters of MR dampers, the simulation results reveal the effectiveness and practicality of the IMRFO in the engineering applications.
The Influence of Liquidity and Solvency on Performance within the Healthcare Industry: Evidence from Publicly Listed Companies
Authors: Batrancea Larissa
Published: September 2021

Any lucrative economic activity implies aiming at obtaining a profit, including companies in the healthcare industry. The present study analyzes the extent to which financial liquidity and financial solvency influenced the performance of 34 healthcare companies that are publicly traded on the New York Stock Exchange. The period of analysis spanned from Q4 2005 to Q4 2020. The research methodology favored a complex approach by running econometric models with two-stage least squares (2SLS) panel and panel generalized method of moments (GMM). Empirical evidence showed that the financial indicators current liquidity ratio, quick liquidity ratio, and debt to equity ratio significantly influenced company performance measured by return on assets, gross margin ratio, operating margin ratio, earnings before interest, tax, depreciation, and amortization. Strategies intended to improve business performance based on liquidity and solvency insights are also addressed.
Evaluating the Optimal External Equity Financing Strategy and Critical Factors for the Startup of Lending Company in Taiwan: An Application of Expert Network Decision Model
Authors: Lin Chun-Yueh , Wang Yi-Hsien
Published: September 2021

During enterprise foundation and development, internal finance and debt finance are of vital importance to start-up entrepreneurs. Therefore, the purpose of this study is mainly to focus on how start-ups can make the optimal evaluation among different external equity crowdfunding solutions and to establish a network decision support model that evaluates the optimal financing solution of start-ups for external equity crowdfunding based on decision science and network architecture. The Lending Company in Financial Technology Industry (LCFTI) was taken as an example. The results indicate that equity crowdfunding is the optimal financing plan in LCFTI. Academically, the results of this study not only help propose a network decision support model using decision science methods and implementing the network analysis to establish an architecture to evaluate the optimal financing plans of start-ups for external equity crowdfunding, they also makes up for the gap in the optimal financing plans of entrepreneurs or start-ups for external equity financing, which has not been specified in the POT theory in the past. Practically, this study provides a useful tool for the entrepreneur of LCFTI to understand the key factors affecting the optimal financing plans for external equity financing and enables LCFTI to measure the optimal financing plans for external equity financing to improve the success rate of finance.
Mathematical Modelling and Study of Stochastic Parameters of Computer Data Processing
Authors: Romansky Radi
Published: September 2021

The main goal of dispatching strategies is to minimize the total time for processing tasks at maximum performance of the computer system, which requires strict regulation of the workload of the processing units. To achieve this, it is necessary to conduct a preliminary study of the applied model for planning. The purpose of this article is to present an approach for automating the investigation and optimization of processes in a computer environment for task planning and processing. A stochastic input flow of incoming tasks for processing is considered and mathematical formalization of some probabilistic characteristics related to the complexity of its servicing has been made. On this basis, a software module by using program language APL2 has been developed to conduct experiments for analytical study and obtaining estimates of stochastic parameters of computer processing and dispatching. The proposed model is part of a generalized environment for program investigation of the computer processing organization and expands its field of application with additional research possibilities.
Convergence and Error Estimation of a New Formulation of Homotopy Perturbation Method for Classes of Nonlinear Integral/Integro-Differential Equations
Authors: Mousa Mohamed M. , Alsharari Fahad
Published: September 2021

In this work, the main concept of the homotopy perturbation method (HPM) was outlined and convergence theorems of the HPM for solving some classes of nonlinear integral, integro-differential and differential equations were proved. A theorem for estimating the error in the approximate solution was proved as well. The proposed HPM convergence theorems were confirmed and the efficiency of the technique was explored by applying the HPM for solving several classes of nonlinear integral/integro-differential equations.
Method Development Progress in Genetic Engineering of Thraustochytrids
Authors: Rau E-Ming , Ertesvåg Helga
Published: September 2021

Thraustochytrids are unicellular, heterotrophic marine eukaryotes. Some species are known to store surplus carbon as intracellular lipids, and these also contain the long-chain polyunsaturated fatty acid docosahexaenoic acid (DHA). Most vertebrates are unable to synthesize sufficient amounts of DHA, and this fatty acid is essential for, e.g., marine fish, domesticated animals, and humans. Thraustochytrids may also produce other commercially valuable fatty acids and isoprenoids. Due to the great potential of thraustochytrids as producers of DHA and other lipid-related molecules, a need for more knowledge on this group of organisms is needed. This necessitates the ability to do genetic manipulation of the different strains. Thus far, this has been obtained for a few strains, while it has failed for other strains. Here, we systematically review the genetic transformation methods used for different thraustochytrid strains, with the aim of aiding studies on strains not yet successfully transformed. The designs of transformation cassettes are also described and compared. Moreover, the potential problems when trying to establish transformation protocols in new thraustochytrid species/strains are discussed, along with suggestions utilized in other organisms to overcome similar challenges. The approaches discussed in this review could be a starting point when designing protocols for other non-model organisms.
Clinical Profile and Aspects of Differential Diagnosis in Patients with ME/CFS from Latvia
Authors: Krumina Angelika , Vecvagare Katrine , Svirskis Simons , Gravelsina Sabine , Nora-Krukle Zaiga , Gintere Sandra , Murovska Modra
Published: September 2021

Background and objectives: There is still an uncertainty regarding the clinical symptomatology and the diagnostic criteria in terms of myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS), as different diagnostic criteria exist. Our aim is to identify the core symptoms of ME/CFS in the outpatient setting in Riga; to distinguish symptoms in patients with ME/CFS and those with symptoms of fatigue; and to investigate patient thoughts on the onset, symptoms, treatment and effect of ME/CFS. Materials and methods: Total of 65 Caucasian patients from an ambulatory care setting were included in the study. Questionnaires, specialist evaluation of the patients and visual analogue scale (VAS) measurements were used to objectify the findings. Results: The study showed that ME/CFS with comorbidities is associated with a more severe disease. A negative correlation was found regarding an increase in age and number of current symptoms, as well as an increase in VAS score and the duration of fatigue and age in the ME/CFS without comorbidities group. Conclusions: Comorbidities tend to present with a more severe course of ME/CFS. Fatigue, myalgia, arthralgia and sleep disturbances tend to be more prevalent in the ME/CFS patients compared to the non-ME/CFS patients. VAS score has a tendency to decrease with age and duration of fatigue. Nonsteroidal anti-inflammatory drugs are the most commonly used pharmacological drug class that reduces ME/CFS symptoms.
The Influence of Maternal Obesity on Cell-Free Fetal DNA and Blood Pressure Regulation in Pregnancies with Hypertensive Disorders
Authors: Stupak Aleksandra , Kwaśniewski Wojciech , Goździcka-Józefiak Anna , Kwaśniewska Anna
Published: September 2021

Background and Objectives: obesity and blood pressure disorders are one of the main risk factors for antenatal, intra, postpartum, and neonatal complications. In preeclampsia (PE), the placental hypoxia leads to vascular endothelium dysfunction, cell necrosis, and apoptosis. This condition is associated with the release of free fetal DNA (cffDNA) circulating in plasma. The disturbance of the efficiency of vasodilatation and blood pressure regulation in PE can be confirmed by analyzing the apelin, salusin, and prosalusin. This study aimed to assess the influence of obesity on cffDNA, and the effectiveness of maintaining normal blood pressure in patients with preeclampsia and gestational hypertension. Material and Methods: the research material was blood serum and oral mucosa swabs, obtained from 168 patients. Pregnant women were divided into the following: a control group (C)—67 women; a gestational hypertension group (GH)—35 patients; a preeclampsia with obesity group (PE + O) (pre-gravid BMI > 30)—23 patients. The rest were lean preeclamptic women (PE)—66 patients—(pre-gravid BMI < 25 in 43 women). Results: the cffDNA was observed in 1.50% of women in the C group, in 2.45% in the GH group, but in 18.18% of lean patients with preeclampsia. The cffDNA was detected in 58% of obese pregnant women with PE. The greater the placental hypoxia was in preeclampsia, the less efficient the hypotensive mechanisms, according to an analysis of the studied adipokines. The prosalusin concentration was significantly lower in the PE group with cffDNA than in the PE group without it (p = 0.008). Apelin was higher in the PE group with cffDNA (p = 0.006) compared to other groups. The same results were also observed in the subgroup with obesity. Conclusion: in preeclamptic women, obesity seems to act as an additive factor of placental damage by means of the dysregulation of hypotensive mechanisms.
Numerical Simulation of Continuous Extraction of Li+ from High Mg2+/Li+ Ratio Brines Based on Free Flow Ion Concentration Polarization Microfluidic System
Authors: Zhang Dongxiang , Zhang Xianglei , Xing Leilei , Li Zirui
Published: September 2021

Ion concentration polarization (ICP) is a promising mechanism for concentrating and/or separating charged molecules. This work simulates the extraction of Li+ ions in a diluted high Mg2+/Li+ ratio salt lake brines based on free flow ICP focusing (FF-ICPF). The model solution of diluted brine continuously flows through the system with Li+ slightly concentrated and Mg2+ significantly removed by ICP driven by external pressure and perpendicular electric field. In a typical case, our results showed that this system could focus Li+ concentration by ~1.28 times while decreasing the Mg2+/Li+ ratio by about 85% (from 40 to 5.85). Although Li+ and Mg2+ ions are not separated as an end product, which is preferably required by the lithium industry, this method is capable of decreasing the Mg2+/Li+ ratio significantly and has great potential as a preprocessing technology for lithium extraction from salt lake brines.