bluuebunny/crossref_metadata_2025 huggingface dataset:
The polypeptide GALNT6 Displays Redundant Functions upon Suppression of its Closest Homolog GALNT3 in Mediating Aberrant O-Glycosylation, Associated with Ovarian Cancer Progression
Authors: Sheta Razan , Bachvarova Magdalena , Macdonald Elizabeth , Gobeil Stephane , Vanderhyden Barbara , Bachvarov Dimcho
Published: May 2019

Epithelial ovarian cancer (EOC) represents the most lethal gynecologic malignancy; a better understanding of the molecular mechanisms associated with EOC etiology could substantially improve EOC management. Aberrant O-glycosylation in cancer is attributed to alteration of N-acetylgalactosaminyltransferases (GalNAc-Ts). Reports suggest a genetic and functional redundancy between GalNAc-Ts, and our previous data are indicative of an induction of GALNT6 expression upon GALNT3 suppression in EOC cells. We performed single GALNT3 and double GALNT3/T6 suppression in EOC cells, using a combination of the CRISPR-Cas9 system and shRNA-mediated gene silencing. The effect of single GALNT3 and double GALNT3/T6 inhibition was monitored both in vitro (on EOC cells roliferation, migration, and invasion) and in vivo (on tumor formation and survival of experimental animals). We confirmed that GALNT3 gene ablation leads to strong and rather compensatory GALNT6 upregulation in EOC cells. Moreover, double GALNT3/T6 suppression was significantly associated with stronger inhibitory effects on EOC cell proliferation, migration, and invasion, and accordingly displayed a significant increase in animal survival rates compared with GALNT3-ablated and control (Ctrl) EOC cells. Our data suggest a possible functional redundancy of GalNAc-Ts (GALNT3 and T6) in EOC, with the perspective of using both these enzymes as novel EOC biomarkers and/or therapeutic targets.
C-Type Natriuretic Peptide: A Multifaceted Paracrine Regulator in the Heart and Vasculature
Authors: Moyes Amie J. , Hobbs Adrian J.
Published: May 2019

C-type natriuretic peptide (CNP) is an autocrine and paracrine mediator released by endothelial cells, cardiomyocytes and fibroblasts that regulates vital physiological functions in the cardiovascular system. These roles are conveyed via two cognate receptors, natriuretic peptide receptor B (NPR-B) and natriuretic peptide receptor C (NPR-C), which activate different signalling pathways that mediate complementary yet distinct cellular responses. Traditionally, CNP has been deemed the endothelial component of the natriuretic peptide system, while its sibling peptides, atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP), are considered the endocrine guardians of cardiac function and blood volume. However, accumulating evidence indicates that CNP not only modulates vascular tone and blood pressure, but also governs a wide range of cardiovascular effects including the control of inflammation, angiogenesis, smooth muscle and endothelial cell proliferation, atherosclerosis, cardiomyocyte contractility, hypertrophy, fibrosis, and cardiac electrophysiology. This review will focus on the novel physiological functions ascribed to CNP, the receptors/signalling mechanisms involved in mediating its cardioprotective effects, and the development of therapeutics targeting CNP signalling pathways in different disease pathologies.
Resveratrol-Coated Balloon Catheters in Porcine Coronary and Peripheral Arteries
Authors: Kamann Stefanie , Haase Tobias , Stolzenburg Nicola , Löchel Melanie , Peters Daniel , Schnorr Jörg
Published: May 2019

Angioplasty aiming at vascular dilatation causes endothelial denudation and induces complex inflammatory responses that affect vascular healing, including delayed reendothelialization and excessive neointima proliferation. Resveratrol is known for multiple beneficial effects on the vessel wall after systemic treatment or sustained release from a stent. It is also used as an additive on drug-coated balloon catheters (DCB). In this study, the effect of a single dose of resveratrol, three days to four weeks after administration as a balloon coating during angioplasty, was investigated. Sixteen pigs underwent angioplasty with resveratrol-coated or uncoated balloon catheters in coronary and peripheral arteries. Vessels were overstretched by approximately 20% to enhance vessel wall injury and to produce persistent vessel wall irritation. A significantly reduced number of micro vessels and macrophages in the adventitia, as well as an improved reendothelialization of the vessel lumen, were observed in resveratrol-treated peripheral arteries. The coronaries had a much higher injury score compared to peripheral vessels. Resveratrol-dependent reduction of macrophages, micro vessels or acceleration of reendothelialization was not evident in the coronary vessels. Additionally, no significant effect on neointima proliferation and inflammation score in either vessel territory was observed as a result of resveratrol treatment. In conclusion, the results suggest that resveratrol diminishes the inflammatory response and promotes vascular healing in peripheral arteries. These same effects are absent in more severely injured coronary arteries.
Molecular Characterization, Bioinformatic Analysis, and Expression Profile of Lin-28 Gene and Its Protein from Arabian Camel (Camelus dromedarius)
Authors: Alharbi Sultan N. , Alduhaymi Ibtehal S. , Alqahtani Lama , Altammaami Musaad A. , Alhoshani Fahad M. , Alrabiah Deema K. , Alyemni Saleh O. , Alsulami Khulud A. , Alghamdi Waleed M. , Fallatah Mohannad
Published: May 2019

Lin-28 is an RNA-binding protein that is known for its role in promoting the pluripotency of stem cells. In the present study, Arabian camel Lin-28 (cLin-28) cDNA was identified and analyzed. Full length cLin-28 mRNA was obtained using the reverse transcription polymerase chain reaction (RT-PCR). It was shown to be 715 bp in length, and the open reading frame (ORF) encoded 205 amino acids. The molecular weight and theoretical isoelectric point (pI) of the cLin-28 protein were predicted to be 22.389 kDa and 8.50, respectively. Results from the bioinformatics analysis revealed that cLin-28 has two main domains: an N-terminal cold-shock domain (CSD) and a C-terminal pair of retroviral-type Cysteine3Histidine (CCHC) zinc fingers. Sequence similarity and phylogenetic analysis showed that the cLin-28 protein is grouped together Camelus bactrianus and Bos taurus. Quantitative real-time PCR (qPCR) analysis showed that cLin-28 mRNA is highly expressed in the lung, heart, liver, and esophageal tissues. Peptide mass fingerprint-mass spectrometry (PMF-MS) analysis of the purified cLin-28 protein confirmed the identity of this protein. Comparing the modeled 3D structure of cLin-28 protein with the available protein 3D structure of the human Lin-28 protein confirmed the presence of CSD and retroviral-type CCHC zinc fingers, and high similarities were noted between the two structures by using super secondary structure prediction.
Boron Deficiency Increases Cytosolic Ca2+ Levels Mainly via Ca2+ Influx from the Apoplast in Arabidopsis thaliana Roots
Authors: Quiles-Pando Carlos , Navarro-Gochicoa M. Teresa , Herrera-Rodríguez M. Begoña , Camacho-Cristóbal Juan J. , González-Fontes Agustín , Rexach Jesús
Published: May 2019

Boron (B) is a micronutrient for plant development, and its deficiency alters many physiological processes. However, the current knowledge on how plants are able to sense the B-starvation signal is still very limited. Recently, it has been reported that B deprivation induces an increase in cytosolic calcium concentration ([Ca2+]cyt) in Arabidopsis thaliana roots. The aim of this work was to research in Arabidopsis whether [Ca2+]cyt is restored to initial levels when B is resupplied and elucidate whether apoplastic Ca2+ is the major source for B-deficiency-induced rise in [Ca2+]cyt. The use of chemical compounds affecting Ca2+ homeostasis showed that the rise in root [Ca2+]cyt induced by B deficiency was predominantly owed to Ca2+ influx from the apoplast through plasma membrane Ca2+ channels in an IP3-independent manner. Furthermore, B resupply restored the root [Ca2+]cyt. Interestingly, expression levels of genes encoding Ca2+ transporters (ACA10, plasma membrane PIIB-type Ca2+-ATPase; and CAX3, vacuolar cation/proton exchanger) were upregulated by ethylene glycol tetraacetic acid (EGTA) and abscisic acid (ABA). The results pointed out that ACA10, and especially CAX3, would play a major role in the restoration of Ca2+ homeostasis after 24 h of B deficiency.
P2X7 Receptor Indirectly Regulates the JAM-A Protein Content via Modulation of GSK-3β
Authors: Wesslau Karl-Philipp , Stein Anabel , Kasper Michael , Barth Kathrin
Published: May 2019

The alveolar epithelial cells represent an important part of the alveolar barrier, which is maintained by tight junction proteins, particularly JAM-A, occludin, and claudin-18, which regulate paracellular permeability. In this study, we report on a strong increase in epithelial JAM-A expression in P2X7 receptor knockout mice when compared to the wildtype. Precision-cut lung slices of wildtype and knockout lungs and immortal epithelial lung E10 cells were treated with bleomycin, the P2X7 receptor inhibitor oxATP, and the agonist BzATP, respectively, to evaluate early changes in JAM-A expression. Biochemical and immunohistochemical data showed evidence for P2X7 receptor-dependent JAM-A expression in vitro. Inhibition of the P2X7 receptor using oxATP increased JAM-A, whereas activation of the receptor decreased the JAM-A protein level. In order to examine the role of GSK-3β in the expression of JAM-A in alveolar epithelial cells, we used lithium chloride for GSK-3β inhibiting experiments, which showed a modulating effect on bleomycin-induced alterations in JAM-A levels. Our data suggest that an increased constitutive JAM-A protein level in P2X7 receptor knockout mice may have a protective effect against bleomycin-induced lung injury. Bleomycin-treated precision-cut lung slices from P2X7 receptor knockout mice responded with a lower increase in mRNA expression of JAM-A than bleomycin-treated precision-cut lung slices from wildtype mice.
Towards A Molecular Understanding of The Cannabinoid Related Orphan Receptor GPR18: A Focus on Its Constitutive Activity
Authors: Sotudeh Noori , Morales Paula , Hurst Dow P. , Lynch Diane L. , Reggio Patricia H.
Published: May 2019

The orphan G-protein coupled receptor (GPCR), GPR18, has been recently proposed as a potential member of the cannabinoid family as it recognizes several endogenous, phytogenic, and synthetic cannabinoids. Potential therapeutic applications for GPR18 include intraocular pressure, metabolic disorders, and cancer. GPR18 has been reported to have high constitutive activity, i.e., activation/signaling occurs in the absence of an agonist. This activity can be reduced significantly by the A3.39N mutation. At the intracellular (IC) ends of (transmembrane helices) TMH3 and TMH6 in GPCRs, typically, a pair of oppositely charged amino acids form a salt bridge called the “ionic lock”. Breaking of this salt bridge creates an IC opening for coupling with G protein. The GPR18 “ionic lock” residues (R3.50/S6.33) can form only a hydrogen bond. In this paper, we test the hypothesis that the high constitutive activity of GPR18 is due to the weakness of its “ionic lock” and that the A3.39N mutation strengthens this lock. To this end, we report molecular dynamics simulations of wild-type (WT) GPR18 and the A3.39N mutant in fully hydrated (POPC) phophatidylcholine lipid bilayers. Results suggest that in the A3.39N mutant, TMH6 rotates and brings R3.50 and S6.33 closer together, thus strengthening the GPR18 “ionic lock”.
Molecular Simulation Study on the Microscopic Structure and Mechanical Property of Defect-Containing sI Methane Hydrate
Authors: Cai Shouyin , Tang Qizhong , Tian Sen , Lu Yiyu , Gao Xuechao
Published: May 2019

The study of changes in the related mechanical property and microscopic structure of methane hydrate during the decomposition process are of vital significance to its exploitation and comprehensive utilization. This paper had employed the molecular dynamics (MD) method to investigate the influence of defects on the microscopic structure and mechanical property of the sI methane hydrate system, and to discover the mechanical property for the defect-containing hydrate system to maintain its brittle materials. Moreover, the stress-strain curve of each system was analyzed, and it was discovered that the presence of certain defects in the methane hydrate could promote its mechanical property; however, the system mechanical property would be reduced when the defects had reached a certain degree (particle deletion rate of 9.02% in this study). Besides, the microscopic structures of the sI methane hydrate before and after failure were analyzed using the F3 order parameter value method, and it was found that the F3 order parameters near the crack would be subject to great fluctuations at the time of failure of the hydrate structure. The phenomenon and conclusions drawn in this study provide a basis for the study of the microscopic structure and mechanical characteristics of methane hydrate.
Including Classical Galactosaemia in the Expanded Newborn Screening Panel Using Tandem Mass Spectrometry for Galactose-1-Phosphate
Authors: Cohen Arieh , Baurek Marta , Lund Allan , Dunø Morten , Hougaard David
Published: May 2019

Galactosaemia has been included in various newborn screening programs since 1963. Several methods are used for screening; however, the predominant methods used today are based on the determination of either galactose-1-phosphate uridyltransferase (GALT) activity or the concentration of total galactose. These methods cannot be multiplexed and therefore require one full punch per sample. Since the introduction of mass spectrometry in newborn screening, many diseases have been included in newborn screening programs. Here, we present a method for including classical galactosaemia in an expanded newborn screening panel based on the specific determination of galactose-1-phosphate by tandem mass spectrometry. The existing workflow only needs minor adjustments, and it can be run on the tandem mass spectrometers in routine use. Furthermore, compared to the currently used methods, this novel method has a superior screening performance, producing significantly fewer false positive results. We present data from 5500 routine newborn screening samples from the Danish Neonatal Screening Biobank. The cohort was enriched by including 14 confirmed galactosaemia positive samples and 10 samples positive for other metabolic disorders diagnosed through the Danish newborn screening program. All galactosaemia positive samples were identified by the method with no false positives. Furthermore, the screening performance for other metabolic disorders was unaffected.
Surface Air Movement: An Important Contributor to Ventilation of Isolated Subsurface Structures?
Authors: McManus Thomas Neil , Haddad Assed
Published: May 2019

This study reports on near-surface airspeed measured using a fast-responding thermoanemometer during an investigation of ventilation of an isolated subsurface structure induced by natural forces. Air speed changes continuously, rapidly, and unpredictably when assessed on the time base of one or two seconds. Zero, the most common air speed, occurred in almost all tests throughout the year but especially during cool and cold months. The most probable non-zero air speed, 10.7 m/min (35 ft/min), occurred in all tests. This air speed is below the level of detection by the senses. The number of zero values and the height of the peak at 10.7 m/min follow a repetitive annual cycle. Isolated subsurface structures containing manhole covers share the characteristics of Helmholtz resonators. Grazing air flow across the opening to the exterior induces rotational air flow in the airspace of a Helmholtz resonator. Rotational flow in the airspace potentially influences the exchange of the confined atmosphere with the external one. Ventilation of the airspace occurs continuously and without cost and is potentially enhanced by the unique characteristics of the Helmholtz resonator excited by surface air movement. These results have immense importance and immediate applicability to worker safety.
Hygiene Defense Behaviors Used by a Fungus-Growing Ant Depend on the Fungal Pathogen Stages
Authors: Bonadies Ernesto , Wcislo William , Gálvez Dumas , Hughes William , Fernández-Marín Hermógenes
Published: May 2019

Parasites and their hosts use different strategies to overcome the defenses of the other, often resulting in an evolutionary arms race. Limited animal studies have explored the differential responses of hosts when challenged by differential parasite loads and different developmental stages of a parasite. The fungus-growing ant Trachymyrmex sp. 10 employs three different hygienic strategies to control fungal pathogens: Grooming the antibiotic-producing metapleural glands (MGs) and planting or weeding their mutualistic fungal crop. By inoculating Trachymyrmex colonies with different parasite concentrations (Metarhizium) or stages (germinated conidia or ungermianted conidia of Metarhizium and Escovopsis), we tested whether ants modulate and change hygienic strategies depending on the nature of the parasite challenge. There was no effect of the concentration of parasite on the frequencies of the defensive behaviors, indicating that the ants did not change defensive strategy according to the level of threat. However, when challenged with conidia of Escovopsis sp. and Metarhizium brunneum that were germinated or not-germinated, the ants adjusted their thygienic behavior to fungal planting and MG grooming behaviors using strategies depending on the conidia germination status. Our study suggests that fungus-growing ants can adjust the use of hygienic strategies based on the nature of the parasites.
Sex Ratio of Small Hive Beetles: The Role of Pupation and Adult Longevity
Authors: Papach Anna , Gonthier Jérémy , Williams Geoffrey R. , Neumann Peter
Published: May 2019

The sex ratio of sexually reproducing animal species tends to be 1:1, which is known as Fisher’s principle. However, differential mortality and intraspecific competition during pupation can result in a biased adult sex ratio in insects. The female-biased sex ratio of small hive beetles (SHBs) is known from both laboratory and field studies, but the underlying reasons are not well understood. Here, we used laboratory mass and individual pupation to test if differential mortality between sexes and/or intraspecific interactions can explain this sex ratio. The data show a significant female-biased adult sex ratio in both mass and individual rearing, even when assuming that all dead individuals were males. Our results therefore suggest that neither differential mortality during pupation nor intraspecific interactions are likely to explain the female-biased sex ratio of freshly emerged adult SHBs. We regard it as more likely that either competition during the larval feeding stage or genetic mechanisms are involved. In addition, we compared our data with previously published data on the sex ratio of both freshly emerged and field-collected SHBs to investigate possible gender differences in adult longevity. The data show a significantly greater female bias in the sex ratio upon emergence, compared to field-collected SHBs, suggesting that adult females have a shorter longevity.
Deep Learning Meets Hyperspectral Image Analysis: A Multidisciplinary Review
Authors: Signoroni Alberto , Savardi Mattia , Baronio Annalisa , Benini Sergio
Published: May 2019

Modern hyperspectral imaging systems produce huge datasets potentially conveying a great abundance of information; such a resource, however, poses many challenges in the analysis and interpretation of these data. Deep learning approaches certainly offer a great variety of opportunities for solving classical imaging tasks and also for approaching new stimulating problems in the spatial–spectral domain. This is fundamental in the driving sector of Remote Sensing where hyperspectral technology was born and has mostly developed, but it is perhaps even more true in the multitude of current and evolving application sectors that involve these imaging technologies. The present review develops on two fronts: on the one hand, it is aimed at domain professionals who want to have an updated overview on how hyperspectral acquisition techniques can combine with deep learning architectures to solve specific tasks in different application fields. On the other hand, we want to target the machine learning and computer vision experts by giving them a picture of how deep learning technologies are applied to hyperspectral data from a multidisciplinary perspective. The presence of these two viewpoints and the inclusion of application fields other than Remote Sensing are the original contributions of this review, which also highlights some potentialities and critical issues related to the observed development trends.
Hydrothermal Aging Properties of Three Typical Bamboo Engineering Composites
Authors: Zhou Haiying , Wang Ge , Chen Linbi , Yu Zhiming , Smith Lee M. , Chen Fuming
Published: May 2019

The objective of this study was to investigate the hygroscopic characteristics of three typical bamboo engineering composites (Bamboo scrimber (BS), bamboo bundle/wood laminated veneer lumber (BLVL), and bamboo laminated timber (BLT)) as well as predict their performance changes and service life in hot humid environments. The composites were subjected to three treatment conditions (23 °C, 63 °C, and 100 °C) for this experiment. The hygroscopic thickness swelling model and Fick’s second law were used to quantify the characterization and prediction of the water absorption, thickness swelling rate, and water absorption rate of BS, BLVL, and BLT. The results indicated that the order of the hygroscopic thickness swelling coefficient KSR and the diffusion coefficient D was BLT > BLVL > BS (at 23 °C and 63 °C). The optimal dimensional stability was displayed by BS, followed by BLVL and BLT. In addition to the hygroscopic properties, elastic modulus degradation was investigated. It was observed that the elastic modulus (MOR) degradation had a linear relationship with the aging temperature. After 152 h of the hydrothermal aging test (63 °C), the MOE of BS, BLVL, and BLT degraded by 44.33%, 53.89%, and 25.83%, respectively.
Thermal Efficiency Analysis for Laser-Assisted Plasma Arc Welding of AISI 304 Stainless Steel
Authors: Hipp Dominik , Mahrle Achim , Beyer Eckhard , Jäckel Sebastian , Hertel Martin , Füssel Uwe
Published: May 2019

Synergistic effects during hybrid laser-arc welding may cause increased process efficiencies. However, the basic interactions behind these effects are still being discussed, with some contradictory reports. In this study, particular welding parameters of interest were systematically varied to further the understanding of involved phenomena. The experimental trials are evaluated regarding their synergistic achievements in terms of process efficiency, melting efficiency and energy coupling efficiency using a factorial two-level Design-of-Experiment (DoE) approach. The results show that the growth in process efficiency can be attributed to a dramatic increase in melting efficiency whereas the energy coupling efficiency is only moderately increased. Thus, the synergistic effect is mainly caused by secondary mechanisms that change the energy usage inside the workpiece while direct interactions between the two heat sources can be excluded as a reasonable cause for increased process efficiencies. It is concluded that the different sizes of the heat sources change the heat and mass flow positively and consequently lead to a higher performance level.
Multi-Objective Optimization Design and Test of Compound Diatomite and Basalt Fiber Asphalt Mixture
Authors: Cheng Yongchun , Li Liding , Zhou Peilei , Zhang Yuwei , Liu Hanbing
Published: May 2019

This study focuses on improving the performance of asphalt mixture at low- and high- temperature and analyzing the effect of diatomite and basalt fiber on the performance of the asphalt mixture. Based on the L16(45) orthogonal experimental design (OED), the content of diatomite (D) and basalt fiber (B) and the asphalt-aggregate (A) ratio were selected as contributing factors, and each contributing factor corresponded to four levels. Bulk volume density (γf), volume of air voids (VV), voids filled with asphalt (VFA), Marshall stability (MS) and splitting strength at −10 °C (Sb) were taken as the evaluation indexes. According to the results of the orthogonal experiment, the range analysis and variance analysis were used to study the effect of the diatomite content, basalt fiber content and asphalt-aggregate ratio on the performance of the asphalt mixture, and the grey correlation grade analysis (GCGA) was used to obtain the optimal mixing scheme. Furthermore, the performance tests were conducted to evaluate the performance improvement of asphalt mixtures with diatomite and basalt fibers, and the scanning electron microscopy (SEM) tests were carried out to analyze the mechanism of diatomite and basalt fibers in asphalt mixtures. The results revealed that the addition of diatomite and basalt fiber can significantly increase the VV of asphalt mixture, and reduce γf and VFA; the optimal performance of the asphalt mixture at high- and low-temperature are achieved with 14% diatomite, 0.32% basalt fibers and 5.45% asphalt-aggregate ratio. Moreover, the porous structure of diatomite and the overlapping network of basalt fibers are the main reasons for improving the performance of asphalt mixture.
Titanium Oxide Microspheres with Tunable Size and Phase Composition
Authors: Poluboyarinov Anton S. , Chelpanov Vitaly I. , Lebedev Vasily A. , Kozlov Daniil A. , Khazova Kristina M. , Volkov Dmitry S. , Kolesnik Irina V. , Garshev Alexey V.
Published: May 2019

Due to their unique physical and chemical properties, monodisperse titanium oxide microspheres can be used in dye-sensitized solar cells, as cosmetic pigments, and for other applications. However, the synthesis of microspheres with narrow size distribution, desired phase composition, and porosity is still a challenge. In this work, spherical titania particles with controllable size, crystallinity, and pore size were obtained by Ti(OnBu)4 hydrolysis in ethanol. The influence of NaOH addition on the particles’ size and morphology was investigated for the first time. Particle diameter can be tailored from 300 nm to 1.5 μm by changing water and NaOH concentrations. Particle size was analyzed by the statistical processing of scanning electron microscopy (SEM) images and differential centrifugal sedimentation (DCS) measurements. Optical properties of the microspheres were studied by diffuse reflectance UV-Vis spectroscopy. Thermal and hydrothermal treatment allowed transforming amorphous phase in as-prepared particles into nanocrystalline anatase and/or rutile. Transmission electron microscopy (TEM) study of the lamellae, cut out from spherical particles using focused ion beam (FIB), revealed that as-synthesized microspheres are non-hollow, homogeneous, and crystallize throughout the whole volume of the particle. The spherical particles possess photoprotective properties; the highest sun protection factor (SPF) was observed for amorphous microspheres.
The Influences of Process Annealing Temperature on Microstructure and Mechanical Properties of near β High Strength Titanium Alloy Sheet
Authors: Du Zhaoxin , Ma Yan , Liu Fei , Xu Ning , Chen Yanfei , Wang Xiaopeng , Chen Yuyong , Gong Tianhao , Xu Dong
Published: May 2019

The influences of process annealing temperature during cold rolling on microstructure and mechanical properties of Ti-3.5Al-5Mo-6V-3Cr-2Sn-0.5Fe near β high strength titanium alloy sheets have been investigated. Results showed that the alloy mainly included the deformation induced dislocation structures after cold rolling but no obvious band structure, twin crystal or martensite were observed in this work. The texture components, which were affected by process annealing, are mainly γ-fiber, α-fiber and weak Goss texture. The γ-fiber of alloy when process annealed at 780 °C (α/β phase field) is stronger than at 830 °C (β phase field), where the Goss texture of alloy with process annealing temperature of 830 °C is more obvious. Results of annealing heat treatments showed that the recrystallization of the cold rolled was basically completed in a relatively short time of 2 min at 750 °C for 2 min. The refinement of grain size led to a significant increase of plasticity compared to rolled alloy. Results of tensile testing of aged alloy display the excellent combination of strength and plasticity, and the cold rolled alloy with process annealed at α/β phase field exhibits the better mechanical properties than at β phase field.
Aging of Bioactive Glass-Based Foams: Effects on Structure, Properties, and Bioactivity
Authors: Menci Pier Francesco , Mari Andrea , Charbonneau Cindy , Lefebvre Louis-Philippe , De Nardo Luigi
Published: May 2019

Bioactive glasses (BG) possess significant bone-bonding and osteogenic properties that support their use for bone defects repair in orthopaedic and dental procedures. Recent advancement enables the manufacturing of BG-based scaffolds providing structural support during bone regeneration. Despite the wide number of studies on BG and BG-based materials, little information on their aging mechanisms and shelf life is available in the literature. In this study, the evolution of chemical species on BG-based foams was investigated via accelerated tests in the presence of CO2 and humidity. The aging process led to the formation of carbonates (Na2CO3 and CaCO3) and hydrocarbonates (NaHCO3). The amount and composition of nucleated species evolved with time, affecting the structure, properties, and bioactivity of the scaffolds. This study provides a first structured report of aging effects on the structure and chemico-physical properties of bioactive glass-based scaffolds, offering an insight about the importance of their storage and packaging.
The Enhancement of H2 Evolution over Sr1−1.5xTbxWO4 Solid Solution under Ultraviolet Light Irradiation
Authors: Yang Jia , Sun Xiaorui , Zeng Ting , Hu Yilan , Shi Jianwei
Published: May 2019

In this work, Sr1−1.5xTbxWO4 (0 ≤ x ≤ 0.2) solid solutions were synthesized via a traditional high-temperature solid state method. Le Bail fitting on the powder X-ray diffraction (XRD) pattern showed that these solid solutions are pure phase. Scanning electron microscopy showed that the SrWO4 and Sr0.82Tb0.12WO4 samples are composed of micrometer particles and submicron crystallites, respectively. Ultraviolet–visible diffuse reflectance spectra suggested that the bandgaps of Sr1−1.5xTbxWO4 are narrower than the undoped sample. The Sr0.82Tb0.12WO4 sample, with the assistance of 1.5 wt % Ru-cocatalyst, exhibits the best performance for H2 evolution in 5 vol % aqueous triethanolamine (TEOA), which results in about 6.1 and 2.8 times efficiency improvement compared with the intrinsic SrWO4 in methanol and aqueous TEOA, respectively. All the photocatalysts recycled after the photocatalytic reactions showed no degradation when checked by powder XRD.
Characteristic Fluctuations of Dynamic Power Delay Induced by Random Nanosized Titanium Nitride Grains and the Aspect Ratio Effect of Gate-All-Around Nanowire CMOS Devices and Circuits
Authors: Li Yiming , Chen Chieh-Yang , Chuang Min-Hui , Chao Pei-Jung
Published: May 2019

In this study, we investigate direct current (DC)/alternating current (AC) characteristic variability induced by work function fluctuation (WKF) with respect to different nanosized metal grains and the variation of aspect ratios (ARs) of channel cross-sections on a 10 nm gate gate-all-around (GAA) nanowire (NW) metal–oxide–semiconductor field-effect transistor (MOSFET) device. The associated timing and power fluctuations of the GAA NW complementary metal–oxide–semiconductor (CMOS) circuits are further estimated and analyzed simultaneously. The experimentally validated device and circuit simulation running on a parallel computing system are intensively performed while considering the effects of WKF and various ARs to access the device’s nominal and fluctuated characteristics. To provide the best accuracy of simulation, we herein calibrate the simulation results and experimental data by adjusting the fitting parameters of the mobility model. Transfer characteristics, dynamic timing, and power consumption of the tested circuit are calculated using a mixed device–circuit simulation technique. The timing fluctuation mainly follows the trend of the variation of threshold voltage. The fluctuation terms of power consumption comprising static, short-circuit, and dynamic powers are governed by the trend that the larger the grain size, the larger the fluctuation.
Formability Limits, Fractography and Fracture Toughness in Sheet Metal Forming
Authors: Magrinho João P. , Silva Maria Beatriz , Reis Luís , Martins Paulo A. F.
Published: May 2019

This paper is focused on the utilisation of double edge notched tension, staggered and shear tests to determine fracture toughness and the formability limits by fracture in principal strain space. The experiments were performed in test specimens with different geometries and ligament angles, and the influence of strain hardening was taken into consideration by selecting two materials (aluminium AA1050-H111 and pure copper), with very different strain hardening exponents. Results are plotted in principal strain space, and the discussion is focused on the link between formability limits, fracture toughness and macroscopic fractography characteristics of the specimens that fail by mode I, mode II or mixed-mode.
Preparation and Properties of Jute Fiber Long-Chain Fatty Acid Esters in Supercritical Carbon Dioxide
Authors: Li Chong , Jiang Yueping , Huang Baoshan , Zhang Menghang , Feng Yanhong , Yang Zhitao
Published: May 2019

A two-step method involving continuous screw-extrusion steam explosion (CSESE) pretreatment and esterification in supercritical carbon dioxide (scCO2) is used to prepare long-chain fatty acid-modified jute fiber. The weight gain percentage (WG %) of CSESE-pretreated jute laurate (JL) was 110.7% when esterification was carried out in scCO2 at 14 MPa and 100 °C for 2 h. The corresponding WG % was 105.5% when esterification was instead carried out in pyridine at 100 °C for 2 h. Scanning electron microscopy and X-ray diffraction indicated that CSESE pretreatment enhanced the reactivity of jute fiber, with esterification in scCO2 simultaneously occurring on the fibers surface and internal walls. The glass transition temperature of esterified jute was approximately 119 °C, indicating that it could be hot processed over a wide temperature range. The esterified jute had an oil absorption ratio of 17.01 g/g, so it can be used as an oil absorption material.
Photoluminescence Characteristics of Sn2+ and Ce3+-Doped Cs2SnCl6 Double-Perovskite Crystals
Authors: Zhang Hongdan , Zhu Ludan , Cheng Jun , Chen Long , Liu Chuanqi , Yuan Shuanglong
Published: May 2019

In recent years, all-inorganic lead-halide perovskites have received extensive attention due to their many advantages, but their poor stability and high toxicity are two major problems. In this paper, a low toxicity and stable Cs2SnCl6 double perovskite crystals were prepared by aqueous phase precipitation method using SnCl2 as precursor. By the XRD, ICP-AES, XPS, photoluminescence and absorption spectra, the fluorescence decay curve, the structure and photoluminescence characteristics of Ce3+-doped and undoped samples have been investigated in detail. The results show that the photoluminescence originates from defects. [ S n S n 4 + 2 + +VCl] defect complex in the crystal is formed by Sn2+ substituting Sn4+. The number of defects formed by Sn2+ in the crystal decreases with Ce3+ content increases. Within a certain number of defects, the crystal luminescence is enhanced with the number of [ S n S n 4 + 2 + +VCl] decreased. When Ce3+ is incorporated into the crystals, the defects of [ C e 3 + S n 4 + +VCl] and [ S n S n 4 + 2 + +VCl] were formed and the crystal show the strongest emission. This provides a route to enhance the photoluminescence of Cs2SnCl6 double perovskite crystals.
Volcanic Mud Incorporating Paper Sludge as Raw Materials for Lightweight Aggregates
Authors: Huang Chung-Ho , Fang Hao-Yu , Chen Han
Published: May 2019

This study assessed the use of volcanic mud collected from southern Taiwan and the incorporation of paper sludge for manufacturing lightweight aggregate (LWA). The firing process of the raw materials and related sintering mechanisms, including sintering temperature and time, were investigated. LWA was manufactured at sintering temperatures ranging from 950 to 1275 °C with soaking times from 2 to 15 min, and preheating temperatures ranged between 500 and 700 °C with soaking times from 5 to 15 min. Using volcanic mud and mixed sludge (volcanic mud with added paper sludge) resulted in the successful manufacture of various qualified LWAs with particle density ranging from 973 to 1950 g/cm3, water absorption from 6.2 to 20.0%, and crushing strength from 2.2 to 15.8 MPa.
Effect of Al–Zn Alloy Coating on Corrosion Fatigue Behavior of X80 Riser Steel
Authors: Han Zhongying , Huang Xiaoguang , Yang Zhicheng
Published: May 2019

This paper presents a corrosion fatigue cyclic failure test for X80 steel, which has arc sprayed with an Al–Zn coating in natural seawater under different stress levels. We found that the Al–Zn coating can significantly improve the corrosion fatigue resistance and slow the crack initiation of X80 steel. The effect of the Al–Zn coating on the corrosion fatigue crack initiation is mainly attributed to its physical isolation, cathodic protection and residual prestress while the effect on crack propagation is due to its inhibition of the formation and evolution of secondary cracks. Moreover, according to the test results, a new life prediction model for corrosion fatigue based on the damage evolution law is proposed and the effect of corrosion–fatigue coupling damage in the proposed model is also considered.
Phytochemical Study of Aerial Parts of Leea asiatica
Authors: Kil Hyun Woo , Rho Taewoong , Yoon Kee Dong
Published: May 2019

Leea asiatica (L.) Ridsdale (Leeaceae) is found in tropical and subtropical countries and has historically been used as a traditional medicine in local healthcare systems. Although L. asiatica extracts have been found to possess anthelmintic and antioxidant-related nephroprotective and hepatoprotective effects, little attention has been paid toward the investigation of phytochemical constituents of this plant. In the current study, phytochemical analysis of isolates from L. asiatica led to the identification of 24 compounds, including a novel phenolic glucoside, seven triterpenoids, eight flavonoids, two phenolic glycosides, four diglycosidic compounds, and two miscellaneous compounds. The phytochemical structures of the isolates from L. asiatica were elucidated using spectroscopic analyses including 1D- and 2D-NMR and ESI-Q-TOF-MS. The presence of triterpenoids and flavonoids supports the evidence for anthelmintic and antioxidative effects of L. asiatica.
Prospective Approach to the Anaerobic Bioconversion of Benzo- and Dibenzothiophene Sulfones to Sulfide
Authors: Senko Olga , Maslova Olga , Gladchenko Marina , Gaydamaka Sergey , Akopyan Argam , Lysenko Sergey , Karakhanov Eduard , Efremenko Elena
Published: May 2019

Sulfur recovery from organic molecules such as toxic sulfones is an actual problem, and its solution through the use of environmentally friendly and nature-like processes looks attractive for research and application. For the first time, the possible bioconversion of organic sulfones (benzo-and dibenzothiophene sulfones) to inorganic sulfide under anaerobic conditions with simultaneous biogas production from glucose within a methanogenesis process is demonstrated. Biogas with a methane content of 50.7%–82.1% was obtained without H2S impurities. Methanogenesis with 99.7%–100% efficiency and 97.8%–100% conversion of benzo- and dibenzothiophene sulfones (up to 0.45 mM) to inorganic sulfide were obtained in eight days by using a combination of various anaerobic biocatalysts immobilized in a poly(vinyl alcohol) cryogel. Pure cell cultures of sulfate-reducing bacteria and/or H2-producing bacteria were tested as additives to the methanogenic activated sludge. The immobilized activated sludge “enhanced” by bacterial additives appeared to retain its properties and be usable multiple times for the conversion of sulfones under batch conditions.
Small Molecule Inhibitors of KDM5 Histone Demethylases Increase the Radiosensitivity of Breast Cancer Cells Overexpressing JARID1B
Authors: Pippa Simone , Mannironi Cecilia , Licursi Valerio , Bombardi Luca , Colotti Gianni , Cundari Enrico , Mollica Adriano , Coluccia Antonio , Naccarato Valentina , La Regina Giuseppe , Silvestri Romano , Negri Rodolfo
Published: May 2019

Background: KDM5 enzymes are H3K4 specific histone demethylases involved in transcriptional regulation and DNA repair. These proteins are overexpressed in different kinds of cancer, including breast, prostate and bladder carcinomas, with positive effects on cancer proliferation and chemoresistance. For these reasons, these enzymes are potential therapeutic targets. Methods: In the present study, we analyzed the effects of three different inhibitors of KDM5 enzymes in MCF-7 breast cancer cells over-expressing one of them, namely KDM5B/JARID1B. In particular we tested H3K4 demethylation (western blot); radio-sensitivity (cytoxicity and clonogenic assays) and damage accumulation (COMET assay and kinetics of H2AX phosphorylation). Results: we show that all three compounds with completely different chemical structures can selectively inhibit KDM5 enzymes and are capable of increasing sensitivity of breast cancer cells to ionizing radiation and radiation-induced damage. Conclusions: These findings confirm the involvement of H3K4 specific demethylases in the response to DNA damage, show a requirement of the catalytic function and suggest new strategies for the therapeutic use of their inhibitors.
New Series of Thiazole Derivatives: Synthesis, Structural Elucidation, Antimicrobial Activity, Molecular Modeling and MOE Docking
Authors: Althagafi Ismail , El-Metwaly Nashwa , Farghaly Thoraya A.
Published: May 2019

Based on the extensive biological activities of thiazole derivatives against different types of diseases, we are interested in the effective part of many natural compounds, so we synthesized a new series of compounds containing di-, tri- and tetrathiazole moieties. The formation of such derivatives proceeded via reaction of 2-bromo-1-(4-methyl-2-(methylamino)thiazol-5-yl)ethan-1-one with heterocyclic amines, o-aminothiophenol and thiosemicarbazone derivatives. The structure and mechanistic pathways for all products were discussed and proved based on spectral results, in addition to conformational studies. Our aim after the synthesis is to investigate their antimicrobial activity against various types of bacteria and fungi species. Preceeding such an investigation, a molecular docking study was carried out with selected conformers, as representative examples, against three pathogen-proteins. This preliminary stage could support the biological application. The potency of these compounds as antimicrobial agents has been evaluated. The results showed that derivatives which have di- and trithiazole rings displayed high activity that exceeds the used standard antibiotic.
Carbazole- and Triphenylamine-Substituted Pyrimidines: Synthesis and Photophysical Properties
Authors: Achelle Sylvain , Rodríguez-López Julián , Larbani Massinissa , Plaza-Pedroche Rodrigo , Robin-le Guen Françoise
Published: May 2019

A series of pyrimidine derivatives bearing one, two or three triphenylamine/9-ethylcarbazole substituents has been synthesized by Suzuki cross-coupling reaction. All compounds showed absorption bands in the UV region and the emission of violet-blue light upon irradiation. Protonation led to quenching of the fluorescence, although some derivatives remained luminescent with the appearance of a new red-shifted band in the spectra. Accurate control of the amount of acid enabled white photoluminescence to be obtained both in solution and in solid state.
Novel 6a,12b-Dihydro-6H,7H-chromeno[3,4-c] chromen-6-ones: Synthesis, Structure and Antifungal Activity
Authors: Bao Jin-ping , Xu Cui-lian , Yang Guo-yu , Wang Cai-xia , Zheng Xin , Yuan Xin-xin
Published: May 2019

A new series of coumarin derivatives, 7-hydroxy-7-(trifluoromethyl)-6a,12b-dihydro-6H,7H-chromeno[3,4-c]chromen-6-ones 3a–p, were synthesized via Michael addition, transesterification and nucleophilic addition from the reaction of 3-trifluoroacetyl coumarins and phenols in the presence of an organic base. The products were characterized by infrared spectroscopy (IR), hydrogen nuclear magnetic resonance spectroscopy (1H-NMR), carbon nuclear magnetic resonance spectroscopy (13C-NMR) and high-resolution mass spectrometer (HRMS). Single crystal X-ray analysis of compounds 3a and 3n clearly confirmed their assigned chemical structures and their twisted conformations. Compound 3a crystallized in the orthorhombic system, Pbca, in which a = 8.6244(2) Å, b = 17.4245(4) Å, c = 22.5188(6) Å, α = 90°, β = 90°, γ = 90°, v = 3384.02(14) Å3, and z = 8. In addition, the mycelial growth rate method was used to examine the in vitro antifungal activities of the title compounds 3a–p against Fusarium graminearum and Fusarium monitiforme at 500 µg/mL. The results showed that compound 3l exhibited significant anti-Fusarium monitiforme activity with inhibitory index of 84.6%.
Silymarin’s Inhibition and Treatment Effects for Alzheimer’s Disease
Authors: Guo Hong , Cao Hui , Cui Xiaowei , Zheng Wenxiu , Wang Shanshan , Yu Jiyang , Chen Zhi
Published: May 2019

As a longstanding problem, Alzheimer’s disease (AD) has stymied researchers in the medical field with its increasing incidence and enormous treatment difficulty. Silymarin has always been valued by researchers for its good efficacy and safety in treating liver disease. Recent studies have shown that silymarin also has good pharmacological activity in the nervous system, especially for the treatment of AD. Silymarin can control the production of Aβ by inhibiting the precursor substance of Aβ (β-amyloid precursor protein), and it can inhibit the polymerization of Aβ. Silymarin can also increase the acetylcholine content in the nervous system by inhibiting cholinesterase activity. At the same time, it also has the effect of resisting oxidative stress and the inflammatory response of the nervous system. These pharmacological activities contribute to the inhibition of the onset of AD. The good efficacy of silymarin on AD and its high safety and availability give it huge potential for the treatment of AD.
Design and Synthesis of Flavonoidal Ethers and Their Anti-Cancer Activity In Vitro
Authors: Jin Lu , Wang Meng-Ling , Lv Yao , Zeng Xue-Yi , Chen Chao , Ren Hai , Luo Heng , Pan Wei-Dong
Published: May 2019

Flavonoids are well-characterized polyphenolic compounds with pharmacological and therapeutic activities. However, most flavonoids have not been developed into clinical drugs, due to poor bioavailability. Herein, we report a strategy to increase the drugability of flavonoids by constructing C(sp2)-O bonds and stereo- as well as regioselective alkenylation of hydroxyl groups of flavonoids with ethyl-2,3-butadienoate allenes. Twenty-three modified flavonoid derivatives were designed, synthesized, and evaluated for their anti-cancer activities. The results showed that compounds 4b, 4c, 4e, 5e, and 6b exhibited better in vitro inhibitory activity against several cancer cell lines than their precursors. Preliminary structure–activity relationship studies indicated that, in most of the cancer cell lines evaluated, the substitution on position 7 was essential for increasing cytotoxicity. The results of this study might facilitate the preparation or late-stage modification of complex flavonoids as anti-cancer drug candidates.