bluuebunny/crossref_metadata_2025 huggingface dataset:
A Systematic Review of Microbiota in Cirrhosis: A Change Towards a More Pathogenic Predisposition
Authors: Xirouchakis Elias , Pelekanos Alexandros , Xirouchakis Spyridon , Kranidioti Hariklia , Manolakopoulos Spilios
Published: January 2025

The microbiome of the human intestine is a regulator of health that modulates immune response and plays an important role in metabolism. The diversity, and abundance of microbiota communities in the gut have been shown to change in cirrhosis and its complications. We aimed to review the current knowledge regarding microbiota alterations in cirrhosis, its potential differences according to etiology, and its role in the development of cirrhosis complications. A systematic search of the online bibliographic database up to July 2024 was performed. Randomized controlled trials and observational and cohort studies that included a total or at least a cohort of cirrhotic adult patients were enlisted for data extraction and analysis. A total of 73 publications were included for data extraction. Alpha diversity was found to decrease in cirrhotic patients in 30/38 (78%) of the studies, while beta diversity in 20/22 (90%) presented significant differences between healthy and cirrhotic groups. Proteobacteria significantly increased in 20/27 (74%) studies, followed by Actinobacteria and Fusobacteria, while 22/25 (88%) studies found either a reduction in cirrhotic patients or increased abundance in healthy controls for Firmicutes and Bacteroidetes. The most abundant genera in hepatic encephalopathy groups were pathobionts such as Enterococcus and Streptococcus, followed by Vellionella and Escherichia. Heterogeneity was found among studies regarding Alpha diversity in hepatocellular carcinoma (HCC) as it was decreased in three studies, indifferent in five, and increased in three studies in comparison to cirrhotic non-HCC patients. The dysbiosis of the gut microbiota is associated with cirrhosis and the development of complications such as hepatic encephalopathy and hepatocellular carcinoma.
The Role of Heat Shock Protein (Hsp) Chaperones in Environmental Stress Adaptation and Virulence of Plant Pathogenic Bacteria
Authors: Figaj Donata
Published: January 2025

Plant pathogenic bacteria are responsible for a substantial number of plant diseases worldwide, resulting in significant economic losses. Bacteria are exposed to numerous stress factors during their epiphytic life and within the host. Their ability to survive in the host and cause symptomatic infections depends on their capacity to overcome stressors. Bacteria have evolved a range of defensive and adaptive mechanisms to thrive under varying environmental conditions. One such mechanism involves the induction of chaperone proteins that belong to the heat shock protein (Hsp) family. Together with proteases, these proteins are integral components of the protein quality control system (PQCS), which is essential for maintaining cellular proteostasis. However, knowledge of their action is considerably less extensive than that of human and animal pathogens. This study discusses the modulation of Hsp levels by phytopathogenic bacteria in response to stress conditions, including elevated temperature, oxidative stress, changes in pH or osmolarity of the environment, and variable host conditions during infection. All these factors influence bacterial virulence. Finally, the secretion of GroEL and DnaK proteins outside the bacterial cell is considered a potentially important virulence trait.
Edentulism or Poor Oral Hygiene: Which Is the Stronger Predictor for All-Cause Mortality?
Authors: Janket Sok-Ja , Kunhipurayil Hasna H. , Tamimi Faleh , Surakka Markku , Li Huiqi , Van Dyke Thomas E. , Meurman Jukka H.
Published: January 2025

Background: All-cause mortality consisting of several heterogeneous subgroups does not have a well-defined set of risk factors. Despite the well-described role of oral hygiene on mortality, the association between the condition of the existing dentition and mortality remains unclear. Therefore, we embarked on the current study to assess the association of oral hygiene self-care (OHS) with all-cause mortality. Methods: We assessed whether edentulism and the levels of OHS are associated with all-cause mortality in 476 subjects without missing values participating in the KOHH study using proportional hazard models. We designated the edentulous group as OHS0, and poor, fair, and good OHS groups as OHS1, OHS2, and OHS3, respectively. The self-reported OHS was validated against clinical measures of oral inflammation and dental cleanliness, i.e., gingival bleeding and plaque indices. We, then, compared all-cause mortality at three levels of OHS (poor, fair, good) to that of the edentulous group. To test whether the association of OHS to all-cause mortality was mediated by inflammation, we adjusted for CRP. Results: The validity of self-reported OHS was good demonstrating an inverse association with gingival inflammation and plaque index in a dose-response manner. The group with good OHS lived significantly longer, with a 50% lower risk of all-cause mortality. The Hazard ratio (HR) = 0.50 (95% confidence limit: 0.25–0.99), p = 0.045, in a model adjusted for age, smoking, body mass index, and education. Adjusting for CRP attenuated the association of OHS to all-cause mortality slightly, suggesting that this association was mediated, at least in part, by inflammation. In the final model, the poor OHS group exhibited HR = 0.98 (0.51–1.89), p = 0.95. The HR and p-value so close to 1 suggested poor OHS has a similar risk to edentulism. Conclusions: OHS was associated with reduced risk for all-cause mortality: the better OHS, the lower the risk for all-cause mortality. Poor oral hygiene showed a similar risk for all-cause mortality to edentulism.
The Crucial Role of the Blood–Brain Barrier in Neurodegenerative Diseases: Mechanisms of Disruption and Therapeutic Implications
Authors: Kim Sehwan , Jung Un Ju , Kim Sang Ryong
Published: January 2025

The blood–brain barrier (BBB) is a crucial structure that maintains brain homeostasis by regulating the entry of molecules and cells from the bloodstream into the central nervous system (CNS). Neurodegenerative diseases such as Alzheimer’s and Parkinson’s disease, as well as ischemic stroke, compromise the integrity of the BBB. This leads to increased permeability and the infiltration of harmful substances, thereby accelerating neurodegeneration. In this review, we explore the mechanisms underlying BBB disruption, including oxidative stress, neuroinflammation, vascular dysfunction, and the loss of tight junction integrity, in patients with neurodegenerative diseases. We discuss how BBB breakdown contributes to neuroinflammation, neurotoxicity, and the abnormal accumulation of pathological proteins, all of which exacerbate neuronal damage and facilitate disease progression. Furthermore, we discuss potential therapeutic strategies aimed at preserving or restoring BBB function, such as anti-inflammatory treatments, antioxidant therapies, and approaches to enhance tight junction integrity. Given the central role of the BBB in neurodegeneration, maintaining its integrity represents a promising therapeutic approach to slow or prevent the progression of neurodegenerative diseases.
Five-Axis Printing of Continuous Fibers on the Mold
Authors: Michalec Paweł , Laux Marius , Srinivas Gidugu Lakshmi , Weidner Robert , Brandstötter Mathias
Published: January 2025

This paper explores a five-axis printing method designed to improve the fabrication of continuous fiber-reinforced thermoplastic composites (CFRTPCs), essential for producing lightweight, complex structures in advanced manufacturing. Traditional CFRTPC placement techniques often face challenges with precision, scalability, and optimal fiber orientation, especially in customized, small-scale applications. The proposed five-axis printing technique overcomes these issues by enabling precise fiber orientation and the production of robust spatial structures using 3D-printed molds compatible with CFRTPCs. Validation through three-point bending and surface quality tests revealed that five-axis printed cylindrical-lattice samples, with fibers oriented at 45°, exhibited superior mechanical properties and surface quality. The five-axis printed samples achieved a load-to-weight ratio 27% higher than traditional samples and maintained their shape even under significant deformation. Surface quality improved significantly, with roughness values reduced from 37.63 µm to approximately 12 µm. This method advances CFRTPC applications in industries requiring complex, lightweight components.
Induced Defense in Ryegrass–Epichloë Symbiosis Against Listronotus bonariensis: Impact on Peramine Levels and Pest Performance
Authors: Chacón-Fuentes Manuel , León-Finalé Gunnary , Lizama Marcelo , Gutiérrez-Gamboa Gastón , Martínez-Cisterna Daniel , Quiroz Andrés , Bardehle Leonardo
Published: January 2025

The Argentine stem weevil (ASW), a major pest in ryegrass pastures, causes significant agricultural losses. Ryegrass can establish a symbiotic association with Epichloë endophytic fungi, which supply chemical defenses, including peramine. This symbiosis helps protect ryegrass by providing peramine, which acts as a primary defense. In addition, ryegrass can activate induced defense mechanisms, with peramine remaining the central agent in response to herbivorous insect attacks. Therefore, this study assessed the feeding of the ASW on ryegrass carrying endophytic fungus and peramine levels in aerial organs and its effects on pest performance. Argentine stem weevil adults and larvae were placed on ryegrass leaves and stems to assess feeding. Two treatments were used: endophyte-free plants and endophyte-colonized plants. After ASW feeding damage, insect consumption was measured by the leaf area consumed. To evaluate peramine production and its increase in response to ASW attack, peramine levels in leaves were analyzed using liquid chromatography. Damaged E+ ryegrass plants showed significant increases in peramine, with adult and larval herbivory raising levels by 291% and 216% in stems and by 135% and 85% in leaves, respectively, compared to controls. Endophyte-free (E−) plants experienced more ASW damage, as insects preferred feeding on them, showing reduced activity as peramine levels rose in endophyte-infected (E+) plants. An oviposition assay confirmed insect preference for endophyte-free (E−) plants. Additionally, larvae reared on endophyte-infected (E+) plants had lower survival rates, correlating negatively with peramine levels. These results emphasize peramine’s role in strengthening ryegrass defenses against ASW, impacting both feeding and larval development.
Sensor-Based Detection of Characteristics of Rubber Springs
Authors: Hrabovský Leopold , Blata Jan , Kovář Ladislav , Kolesár Michal , Štěpáník Jaromír
Published: January 2025

Knowledge of experimentally obtained values of elastic deformations of rubber springs induced by applied compressive forces of known magnitudes is essential for the selection of rubber springs with optimal properties, which are used to dampen vibrations transmitted to the supporting parts of vibrating machines. This paper deals with the laboratory measurement of the characteristics of rubber springs using two types of sensors which sense the instantaneous value of the compressive force acting on the compressed spring. When using a strain tensometric force sensor, the magnitude of the measured pressure forces was evaluated by the DeweSoft DS-NET system, which was connected to an ethernet LAN, so the measured data could be processed, analysed and stored by any computer on the network. The characteristics of eight types of rubber springs were measured in two ways on laboratory equipment, and the spring stiffnesses were calculated from the measured data. Experiments have shown that the actual stiffnesses of rubber springs are lower compared to the values stated by the manufacturer, in the least favourable case, by 33.6%. It has been shown by measurements that at the beginning of the loading of the rubber spring, its compression is gradual, and the stiffness increases slowly, which is defined as the progressivity of the spring.
Controlling Magnetization in Ferromagnetic Semiconductors by Current-Induced Spin-Orbit Torque
Authors: Lee Sanghoon , Liu Xinyu , Furdyna Jacek
Published: January 2025

In this paper, we review our work on the manipulation of magnetization in ferromagnetic semiconductors (FMSs) using electric-current-induced spin-orbit torque (SOT). Our review focuses on FMS layers from the (Ga,Mn)As zinc-blende family grown by molecular beam epitaxy. We describe the processes used to obtain spin polarization of the current that is required to achieve SOT, and we briefly discuss methods of specimen preparation and of measuring the state of magnetization. Using specific examples, we then discuss experiments for switching the magnetization in FMS layers with either out-of-plane or in-plane easy axes. We compare the efficiency of SOT manipulation in single-layer FMS structures to that observed in heavy-metal/ferromagnet bilayers that are commonly used in magnetization switching by SOT. We then provide examples of prototype devices made possible by manipulation of magnetization by SOT in FMSs, such as read-write devices. Finally, based on our experimental results, we discuss future directions which need to be explored to achieve practical magnetic memories and related applications based on SOT switching.
Optimal Blend Between Fluorinated Esters and Fluorinated Ether for High-Performance Lithium-Ion Cells at High Voltage
Authors: Sheng Yong , Liu Bo , He Junjiang , Zhi Maoyong , Ouyang Dongxu
Published: January 2025

An experimental investigation is conducted to identify the optimal blend of fluoroethylene carbonate (FEC), 3,3,3-trifluoropropylene carbonate (TFEC), and various fluorinated ethers, including 1,1,2,2-tetrafluoroethyl-2,2,2-trifluoroethyl ether (HFE), 1,1,2,2-tetrafluoroethyl-2,2,3,3-tetrafluoropropyl ether (TTE), and bis(2,2,2-trifluoroethyl) ether (BTE), to enhance the performances of lithium-ion cells at high voltage. The cell incorporating TTE exhibits a significantly superior capacity for retention after long-term cycling at 4.5 V, which might be attributed to the improved kinetics of lithium ions and the generation of a thin, reliable, and inorganic-rich electrode–electrolyte interface. This enhancement facilitates greater lithium ion mobility within the cell, while effectively suppressing active lithium loss and side reactions between the electrodes and electrolytes at elevated voltages. Furthermore, the cell with TTE demonstrates a superior rate capability and high-temperature performance. As a result of the inherent safety characteristics of these all-fluorinated electrolytes, cells using these formulations show excellent safety properties under typical abuse scenarios. Except at elevated temperatures, none of the cells undergo thermal runaway when subjected to mechanical or electrical abuse, and there are minimal differences in safety performance across the different formulations. Considering electrochemical performance, safety, and cost factors, it can be concluded that TTE might be more optimal to cooperate with FEC and TFEC for high-performance high-voltage cells.
Influence of Processing Parameters on Flow Behaviour of Ultra-Large-Section Beam Blank Continuous Casting Mould
Authors: Deng Yulong , Deng Nanzhou , Duan Jintao , Li Yibo , Gao Qi , Ni Weihua , Peng Jingquan
Published: January 2025

The complex cross-sectional shape of oversized beam blanks and the size effect of ultra-large-section beam blanks create severe issues related to the surface and internal quality of the castings. To ensure quality and control in the production of ultra-large-section beam blanks, a numerical and physical model of molten steel flow in the three-port submerged entrance nozzle (SEN) mould, with section dimensions of 1300 × 510 × 140 mm, was established. This model was created using numerical simulations and NSGA-II genetic algorithm optimisation, and the impact of the casting speed and SEN immersion depth on the mould’s flow behaviour was investigated. The results showed that a deeper SEN immersion depth resulted in, a greater impact depth of the molten steel, and the surface flow velocity decreased. Both the impact depth and the surface flow velocity of the molten steel increased with increasing casting speed. The physical simulation and numerical simulation of the molten steel flow form and flow velocity distribution in the mould were in good agreement with each other, thus verifying the accuracy of the numerical simulation. The process parameters derived from this study were all within an appropriate range, which can help to improve the quality of continuously cast beam blanks. This also provides guidance for selecting optimal parameters for actual continuous casting production processes.
Experimental Study on Deep-Drawing Dies Made of Pre-Stressed UHPC
Authors: Holzer Katja , Zhang Yuqi , Martinitz Lukas , Hoyer Julika , Volk Wolfram
Published: January 2025

Deep drawing is a cost-efficient way of producing sheet metal parts in high production volumes. Prototypes and very small series are expensive due to the cost of steel-forming tools. Ultra-high-performance concrete (UHPC) tools offer a cheap and fast alternative to conventional steel-forming tools. However, the flexural and tensile strength of UHPC limits its use in complex loading situations occurring in the forming die during deep drawing. To overcome this challenge, we propose pre-stressing UHPC by using expansive UHPC in a mechanically restrained condition. An aluminum powder-based expansive agent can be used to induce a free volume increase in UHPC specimens. With push-out test specimens, the increase in strength was tested when restraining the free volume increase with a steel reinforcement. After 3 and 12 months, no notable pre-straining effect could be measured when restraining the expansion of UHPC. Nonetheless, in deep-drawing experiments, the die made of expansive UHPC in a restrained condition withstood a maximal load during deep drawing of 110 kN. Compared to the die made from UHPC only, the failure mode changed from complete fracture to surface degradation of the drawing radius after 15 strokes.
Exploring the Performance of Some Efficient Explicit Numerical Methods with Good Stability Properties for Huxley’s Equation
Authors: Khayrullaev Husniddin , Omle Issa , Kovács Endre
Published: January 2025

Four explicit numerical schemes are collected, which are stable and efficient for the diffusion equation. Using these diffusion solvers, several new methods are constructed for the nonlinear Huxley’s equation. Then, based on many successive numerical case studies in one and two space dimensions, the least performing methods are gradually dropped out to keep only the best ones. During the tests, not only one but all the relevant time step sizes are considered, and for them, running-time measurements are performed. A major aspect is computational efficiency, which means that an acceptable solution is produced in the shortest possible time. Parameter sweeps are executed for the coefficient of the nonlinear term, the stiffness ratio, and the length of the examined time interval as well. We obtained that usually, the leapfrog–hopscotch method with Strang-type operator-splitting is the most efficient and reliable, but the method based on the Dufort–Frankel scheme can also be very efficient.
Etiological Aspects of Infectious Endocarditis in a Tertiary Hospital in Northeastern Romania
Authors: Loghin Isabela Ioana , Surdu Amelia Elena , Rusu Șerban Alin , Cecan Ion , Dorobăț Victor Daniel , Mihăescu Amelia Andreea , Dorobăţ Carmen Mihaela
Published: January 2025

Background and Objectives: Infective endocarditis (IE) is a severe, life-threatening infection of the endocardial surface. Its incidence has shifted towards older, immunocompromised patients and those with cardiac devices. Materials and Methods: This study was conducted at the “Sf. Parascheva” Clinical Hospital of Infectious Diseases in Iasi, Romania, and retrospectively analyzed cases of IE from January 1, 2019, to September 30, 2024. It received ethical approval (Approval No. 7/17 June 2024). Results: The study included 130 patients with infectious endocarditis, predominantly men (75.38%), with a median age of 55 years. The most affected age groups were 50–59 and over 60 years, each representing 30.76% of cases. The most frequently implicated etiological agent was Staphylococcus aureus in 33% of cases. The most common antibiotic regimen combined glycopeptides and fluoroquinolones/polymyxins (27% cases). Conclusions: A multidisciplinary approach involving infectious disease specialists, cardiologists, and cardiovascular surgeons is essential for effective treatment. Immediate combined antibiotic therapy is vital for presumed IE cases. Despite advances in diagnosis and treatment, the high mortality rates highlight the importance of timely intervention. Future research should focus on improving preventive and therapeutic strategies for IE.
A Review of Sulfate Removal from Water Using Polymeric Membranes
Authors: Al Mehrate Jamal , Shaban Sadek , Henni Amr
Published: January 2025

Access to clean and reliable water has become a critical concern due to the global water crisis. High sulfate levels in drinking water raise health concerns for humans and animals and can cause serious corrosion in industrial systems. Sulfated waters represent a major challenge on the Canadian prairies, leading to many cattle deaths. While reverse osmosis (RO) membranes effectively remove sulfates, they are costly due to high-pressure requirements. Nanofiltration (NF) membranes present a more affordable alternative, outperforming traditional methods like adsorption, desalination, and ion exchange. Developing low-pressure ultrafiltration (UF) and microfiltration (MF) membranes could also reduce costs. This review explores advancements in polymeric materials and membrane technology to enhance sulfate removal, focusing on methods used to reduce fouling and improve permeate flux. Techniques discussed include phase inversion (PI), thin-film composite (TFC), and thin-film nanocomposite (TFN) membranes. The review also highlights recent fabrication methods for pristine and nanomaterial-enhanced membranes, acknowledging both benefits and limitations. Continued innovations in polymer-based membranes are expected to drive further performance and cost-efficiency improvements. This review found that studies in the literature dealt mainly with sulfate concentrations below 2000 mg/L, indicating a need to address higher concentrations in future studies.
Membrane Treatment to Improve Water Recycling in an Italian Textile District
Authors: Tuci Francesca , Allocca Michele , Fibbi Donatella , Daddi Daniele , Gori Riccardo
Published: January 2025

The textile district of Prato (Italy) has developed a wastewater recycling system of considerable scale. The reclaimed wastewater is characterized by high levels of hardness (32 °F on average), which precludes its direct reuse in numerous wet textile processes (e.g., textile dyeing). Consequently, these companies utilize ion exchange resins for water softening. However, the regeneration of the resins results in an increased concentration of chlorides in the reclaimed wastewater that exceeds the limit set by Italian regulations for the reuse of water for irrigation purposes. The objective of this study is to investigate the potential of membrane filtration as an alternative method for removing hardness from water. Therefore, an industrial-scale ultrafiltration-nanofiltration (UF-NF) pilot plant was installed to test the rejection of hardness from the reclaimed wastewater. The experiment employed two types of NF membranes and three permeate fluxes (27, 35, and 38 L·m−2·h−1) for testing. The results demonstrated that the system could remove hardness with efficiencies exceeding 98% under all conditions tested. The experimental findings indicate that the UF-NF system has the potential to be employed as a post-treatment step to render the reclaimed wastewater suitable for all textile finishing processes and to expand the scope for reuse.
Reduced Presence of SARS-CoV-2 microRNA-like Small RNA in the Serum of Patients with Post-Acute Sequelae SARS-CoV-2 Infection
Authors: Stincarelli Maria Alfreda , Abbate Isabella , Matusali Giulia , Tanturli Michele , Camici Marta , Arvia Rosaria , Lazzari Elisabetta , Cimini Eleonora , Vergori Alessandra , Maggi Fabrizio , Giannecchini Simone
Published: January 2025

The mechanisms underlying post-acute sequelae of SARS-CoV-2 infection (PASC) are a topic of debate. This study examined the presence of SARS-CoV-2 microRNA (miRNA)-like small RNAs in extracellular fluids and their potential link to PASC by using a quantitative stem-loop RT-PCR MiRNA assay. Initially, it was demonstrated that three previously identified SARS-CoV-2 miRNA-like small RNAs, specifically svRNA 1 and 2 and miR-07a, were significantly expressed in infected cells in vitro and released into the supernatant following infection by different SARS-CoV-2 variants. Then, the expression of three SARS-CoV-2 small RNAs was studied in both nasopharyngeal swabs (NPS) and sera from 24 patients at their initial COVID-19 diagnosis (T0) and in sera collected 91 to 193 days post-diagnosis (T1). Notably, 11 out of 24 patients (46%) reported PASC consequences. All NPS samples showed SARS-CoV-2 small RNA expression with an altered cytokine network during acute infection, but it did not correlate with PASC outcomes. Serum samples had similar small RNA statuses, though PASC patients, notably at T1, but not at T0, displayed reduced overall positivity compared to those without PASC. The host target expression of SARS-CoV-2 small RNAs was not significantly different between groups. This suggests a need for further research into SARS-CoV-2 small RNA and its role in viral behavior and PASC consequences.
Human–Robot Interactions: A Pilot Study of Psychoaffective and Cognitive Factors to Boost the Acceptance and Usability of Assistive Wearable Devices
Authors: Bertuccelli Margherita , Tortora Stefano , Trombin Edoardo , Negri Liliana , Bisiacchi Patrizia , Menegatti Emanuele , Del Felice Alessandra
Published: January 2025

Robotic technology to assist rehabilitation provides practical advantages compared with traditional rehabilitation treatments, but its efficacy is still disputed. This controversial effectiveness is due to different factors, including a lack of guidelines to adapt devices to users’ individual needs. These needs include the specific clinical conditions of people with disabilities, as well as their psychological and cognitive profiles. This pilot study aims to investigate the relationships between psychological, cognitive, and robot-related factors playing a role in human–robot interaction to promote a human-centric approach in robotic rehabilitation. Ten able-bodied volunteers were assessed for their anxiety, experienced workload, cognitive reserve, and perceived exoskeleton usability before and after a task with a lower-limb exoskeleton (i.e., 10 m path walking for 10 trials). Pre-trial anxiety levels were higher than post-trial ones (p < 0.01). While trait anxiety levels were predictive of the experienced effort (Adjusted-r2 = 0.43, p = 0.02), the state anxiety score was predictive of the perceived overall workload (Adjusted-r2 = 0.45, p = 0.02). High–average cognitive reserve scores were predictive of the perception of exoskeleton usability (Adjusted-r2 = 0.45, p = 0.02). A negative correlation emerged between the workload and the perception of personal identification with the exoskeleton (r = −0.67, p-value = 0.03). This study provides preliminary evidence of the impact of cognitive and psychoaffective factors on the perception of workload and overall device appreciation in exoskeleton training. It also suggests pragmatic measures such as familiarization time to reduce anxiety and end-user selection based on cognitive profiles. These assessments may provide guidance on the personalization of training.
Various Sizes and Shapes of Mixed-Anion Fe(NH2trz)3(BF4)2−x(SiF6)x/2@SiO2 Nanohybrid Particles Undergoing Spin Crossover Just Above Room Temperature
Authors: Yang Xinyu , Bielas Rafal , Collière Vincent , Salmon Lionel , Bousseksou Azzedine
Published: January 2025

Spin crossover (SCO) iron (II) coordination compounds in the form of nanohybrid SCO@SiO2 particles were prepared using a reverse micelles technique based on the TritonX-100/cyclohexane/water ternary system. Tetraethyl orthosilicate (TEOS) acts as precursor of both the SiF62− counter-anion and SiO2 to obtain Fe(NH2trz)3(BF4)2−x(SiF6)x/2@SiO2 nanoparticles with different sizes and morphologies while modifying the TEOS concentration and reaction time. The adjustable mixed-anion strategy leads to a range of quite scarce abrupt spin crossover behaviors with hysteresis just above room temperature (ca. 293 K), which is very promising for the integration of these materials into functional devices.
Significance of the Powder Metallurgy Approach and Its Processing Parameters on the Mechanical Behavior of Magnesium-Based Materials
Authors: Sharma Sachin Kumar , Gajević Sandra , Sharma Lokesh Kumar , Mohan Dhanesh G. , Sharma Yogesh , Radojković Mladen , Stojanović Blaža
Published: January 2025

Magnesium-based materials, which are known for their light weight and exceptional strength-to-weight ratio, hold immense promise in the biomedical, automotive, aerospace, and military sectors. However, their inherent limitations, including low wear resistance and poor mechanical properties, have driven the development of magnesium-based metal matrix composites (Mg-MMCs). The pivotal role of powder metallurgy (PM) in fabricating Mg-MMCs was explored, enhancing their mechanical and corrosion resistance characteristics. The mechanical characteristics depend upon the fabrication methodology, composition, processing technique, and reinforcement added to the magnesium. PM is identified as the most efficient due to its ability to produce near-net shape composites with high precision, cost-effectiveness, and minimal waste. Furthermore, PM enables precise control over critical processing parameters, such as compaction pressure, sintering temperature, and particle size, which directly influence the composite’s microstructure and properties. This study highlights various reinforcements, mainly carbon nanotubes (CNTs), graphene nanoparticles (GNPs), silicon carbide (SiC), and hydroxyapatite (HAp), and their effects on improving wear, corrosion resistance, and mechanical strength. Among these, CNTs emerge as a standout reinforcement due to their ability to enhance multiple properties when used at optimal weight fractions. Further, this study delves into the interaction between reinforcement types and matrix materials, emphasizing the importance of uniform dispersion in preventing porosity and improving durability. Optimal PM conditions, such as a compaction pressure of 450 MPa, sintering temperatures between 550 and 600 °C, and sintering times of 2 h, are recommended for achieving superior mechanical performance. Emerging trends in reinforcement materials, including nanostructures and bioactive particles, are also discussed, underscoring their potential to widen the application spectrum of Mg-MMCs.
Stable Field Emissions from Zirconium Carbide Nanoneedle Electron Source
Authors: Wu Yimeng , Tang Jie , Tang Shuai , Chen You-Hu , Chiu Ta-Wei , Takeguchi Masaki , Hashimoto Ayako , Qin Lu-Chang
Published: January 2025

In this study, a single zirconium carbide (ZrC) nanoneedle structure oriented in the <100> direction was fabricated by a dual-beam focused ion beam (FIB-SEM) system, and its field emission characteristics and emission current stability were evaluated. Benefiting from controlled fabrication with real-time observation, the ZrC nanoneedle has a smooth surface and a tip with a radius of curvature smaller than 20 nm and a length greater than 2 μm. Due to its low work function and well-controlled morphology, the ZrC nanoneedle emitter, positioned in a high-vacuum chamber, was able to generate a single and collimated electron beam with a current of 1.2 nA at a turn-on voltage of 210 V, and the current increased to 100 nA when the applied voltage reached 325 V. After the treatment of the nanoneedle tip, the field emission exhibited a stable emission for 150 min with a fluctuation of 1.4% and an emission current density as high as 1.4 × 1010 A m−2. This work presents an efficient and controllable method for fabricating nanostructures, and this method is applicable to the transition metal compound ZrC as a field emission emitter, demonstrating its potential as an electron source for electron-beam devices.
Influence of Added Cellulose Nanocrystals on the Rheology of Polymers
Authors: Pal Rajinder , Deshpande Parth , Patel Smit
Published: January 2025

The interactions between cellulose nanocrystals and six different polymers (three anionic, two non-ionic, and one cationic) were investigated using rheological measurements of aqueous solutions of nanocrystals and polymers. The experimental viscosity data could be described adequately by a power-law model. The variations in power-law parameters (consistency index and flow behavior index) with concentrations of nanocrystals and polymers were determined for different combinations of nanocrystals and polymers. The interactions between nanocrystals and the following polymers: anionic sodium carboxymethyl cellulose and non-ionic guar gum, were found to be strong in that the consistency index increased substantially with the addition of nanocrystals to polymer solutions. The interaction between nanocrystals and non-ionic polymer polyethylene oxide was moderate. Depending on the concentrations of nanocrystals and polymer, the consistency index both increased and decreased upon the addition of nanocrystals to polymer solution. The interactions between nanocrystals and the following polymers: anionic xanthan gum, anionic polyacrylamide, and cationic quaternary ammonium salt of hydroxyethyl cellulose, were found to be weak. The changes in rheological properties with nanocrystal addition to these polymer solutions were found to be small or negligible.
HIV Drug Resistance Profile in Clients Experiencing Treatment Failure After the Transition to a Dolutegravir-Based First-Line Antiretroviral Treatment Regimen in Mozambique
Authors: Ismael Nalia , Hussein Cidia , Magul Cacildo , Inguane Humberto , Couto Aleny , Nhangave Amancio , Muteerwa Ana , Bonou Mahoudo , Ramos Artur , Young Peter Wesley , Chilundo Sonia , Machekano Rhoderick , Greenberg Lauren , da Silva Juliana , Bhatt Nilesh
Published: January 2025

Real-world data on HIV drug resistance (HIVDR) after transitioning to tenofovir disoproxil fumarate/lamivudine/dolutegravir (TLD) are limited. We assessed HIVDR rates and patterns in clients with virological failure (VF) after switching from an NNRTI-based regimen to TLD. A cross-sectional study was conducted in Gaza, Mozambique (August 2021–February 2022), including adults on first-line ART for ≥12 months who transitioned to TLD and had unsuppressed viral load (VL) ≥ 1000 copies/mL six months post-transition. After three adherence counseling sessions, participants with VF underwent genotyping for drug resistance mutations (DRMs) using the Stanford HIVdb Program. Of 717 participants (median age 39.2 years, 70.7% female), 217 (30.2%) had VF, 193 (88.9%) underwent genotyping, with 183 (94.8%) successfully genotyped. Intermediate–high dolutegravir (DTG) resistance was found in 19.6% (36/183). Unsuppressed VL before DTG transition was independently associated with VF (aOR: 2.14). Resistance patterns included 33.3% (12/36; 95% CI: 14.6–46.3) to all three TLD drugs, 55.6% (20/36; 95% CI: 39.3–71.9) to DTG and 3TC, and 11% (4/36; 95% CI: 0.8–21.3) to DTG only. Major drug resistance mutations to DTG included G118R (9.3%), R263K (6.6%), and Q148H/R/K (4.4%). This study highlights the need to consider virologic status before transitioning PLHIV to TLD and suggests that adherence counseling may not prevent resistance in those with unknown or prior VF.
3D-Printed Tablets of Nifurtimox: In Vitro and In Vivo Anti-Trypanosoma cruzi Studies
Authors: Bedogni Giselle R. , Lima Ana Luiza , Gross Idejan P. , Menezes Tatiana Prata , Talvani Andre , Cunha-Filho Marcilio , Salomon Claudio J.
Published: January 2025

Background/Objectives: Chagas disease is a neglected tropical disease caused by infection with the parasite Trypanosoma cruzi. Benznidazole and nifurtimox are the only approved drugs for treating this condition, but their low aqueous solubility may lead to erratic bioavailability. This work aimed for the first time to formulate tablets of nifurtimox by hot melt extrusion coupled with 3D printing as a strategy to increase drug dissolution and the production of tablets with dosage on demand. Methods: Different pharmaceutical-grade polymers were evaluated through film casting, and those with promising nifurtimox amorphization capacity were further used to prepare filaments by hot melt extrusion. The printability of the obtained filaments was tested, and the polyvinyl alcohol filament was further used for printing tablets containing 120 and 60 mg of nifurtimox. Results: Three-dimensional tablets showed a remarkable improvement in the drug dissolution rate compared to commercial tablets and a dissolution efficiency 2.8 times higher. In vivo studies were carried out on Swiss mice. Parasitemia curves of nifurtimox printed tablets were significantly superior to the pure drug. Moreover, NFX 3D tablets provided a similar Trypanosoma cruzi reduction in plasmatic concentration to benznidazole, the gold-standard drug for acute-phase treatment of the Chagas disease. Conclusions: The findings of this work showed that hot melt extrusion coupled with 3D printing is a promising alternative for increasing nifurtimox biopharmaceutical properties and an attractive approach for personalized medicine.
Performance Analysis of Buffer-Aided FSO Relaying with an Energy Harvesting Relay
Authors: Abou-Rjeily Chadi
Published: January 2025

In this paper, we consider a three-node free space optical (FSO) decode-and-forward (DF) cooperative network. The relay is not connected to a permanent power supply and relies solely on the optical energy harvested (EH) from the source node. This energy is accumulated in an energy buffer in order to enable the relay–destination communications. Moreover, buffer-aided (BA) relaying is considered where the relay is equipped with a data buffer for storing the incoming packets. For such networks, we propose a relaying protocol that delineates the roles of the source and the EH BA relay in each time slot. We develop a Markov chain framework for capturing the dynamics of the data and energy buffers. We derive the transition probabilities between the states of the Markov chain after discretizing the continuous-value energy buffer allowing for the evaluation of the analytical performance of the considered system. A numerical analysis is also presented over a turbulence-induced gamma–gamma fading channel highlighting the impacts of the data rate threshold levels, relay position, relay transmit power and propagation conditions on the achievable performance levels. Results validate the accuracy of the theoretical analysis and demonstrate significant reductions in the network outage, especially when the relay’s transmit level is appropriately selected.
Bryophyte Flora of AlUla County (Saudi Arabia)—Distribution, Ecology, and Conservation
Authors: Hugonnot Vincent , Pépin Florine , Freedman Jan
Published: January 2025

Intensive surveys recently conducted in AlUla County (northwest Saudi Arabia) have made it possible to compile an exhaustive list of the forty-eight bryophyte species (six liverworts and forty-two mosses) known in this region and compare it with an updated checklist of bryophytes from Saudi Arabia, which now counts 31 liverworts and 135 mosses. The ecology, taxonomy, and distribution of each taxon are provided and discussed in a systematic catalog. Although particularly arid and lacking permanent watercourses, AlUla County has proven to be surprisingly rich in bryophytes. Some remarkable species are even confined here, such as Riella affinis, Acaulon triquetrum, Microbryum rectum, and Syntrichia rigescens. Two endemic species were also observed. An updated checklist of Arabian bryophyte endemics is provided. Specific recommendations regarding the conservation of bryophyte heritage are outlined.
Enhancing Acclimatization Conditions for Vriesea splendens ‘Fire’: A Comparative Analysis of Substrate Effects on Growth and Survival
Authors: Eisa Eman Abdelhakim , Pasquel Davila Daniela Salome , Ördögh Máté
Published: January 2025

This study investigates the acclimatization success of Vriesea splendens ’Fire’, a popular ornamental bromeliad, through in vitro propagation on various substrates. Due to the increasing demand for V. splendens, micropropagation offers a promising solution to overcome the limitations of traditional propagation methods. In this research, acclimatization was conducted in two trial types: in the one-step greenhouse conditions, and in two-step acclimatization, which introduced a controlled laboratory step before transferring plants to the greenhouse. The substrates examined included pure and mixed forms of turf, perlite, coco coir, pine bark (hereafter referred to as bark), moss, and vermiculite. Morphological traits such as plant height, leaf length, number and length of roots, and fresh weight were evaluated, together with physiological parameters, such as chlorophyll and carotenoid concentrations and survival percentage, to test the effectiveness of acclimatization. Coco coir-based substrates significantly enhanced plant height, root development, and survival percentages in both experiments compared with other substrates, thus proving its suitability for the propagation of V. splendens. Vermiculite had the highest survival rate during one-step acclimatization, whereas turf showed a very good performance in two-step acclimatization. On the opposite side, substrates containing bark and moss showed a reduced effect on plant growth and survival, which indicated the vital role of substrates for best development. Statistical analyses confirmed the superiority of some combinations of substrates related to physiological health, showing that optimal acclimatization results could be improved by a chosen substrate. These results strengthen the present in vitro propagation protocols of the Vriesea species by confirming the relevance of substrate choice in producing hardy plants with good commercial prospects.
Influence of Monomer Size on CO2 Adsorption and Mechanical Properties in Microporous Cyanate Ester Resins
Authors: Bai Yukun , Kikugawa Gota , Kishimoto Naoki
Published: January 2025

Molecular simulations offer valuable insights into thermosetting polymers’ microstructures and interactions with small molecules, aiding in the development of advanced materials. In this study, we design two cyanate resin models featuring monomers of different sizes and employ a previously developed method to generate crosslinked structures. We then analyze their crosslinking processes and physicochemical properties. Using quantum chemistry calculations and a GCMC/MD approach, we investigate CO2 adsorption. Our results show that monomer size does not significantly affect the crosslinking process and provides a degree of polymerization as 83.8 ± 0.3% vs. 76.7 ± 1.4%, but it does influence key properties, such as the glass transition temperature (520 K vs. 420 K) and Young’s modulus (2.32 GPa vs. 1.77 GPa). Moreover, CO2 adsorption differs between the two models: the introduction of propyl ether moieties lowers by around 70% CO2 uptake, indicating that specific adsorption sites impact gas adsorption. This study demonstrates a promising strategy for designing and optimizing thermosetting polymers with controllable gas separation and storage capabilities.
Ultrasonic Deposition of Cellulose Nanocrystals on Substrates for Enhanced Eradication Activity on Multidrug-Resistant Pathogens
Authors: Jabreen Lama , Maruthapandi Moorthy , Durairaj Arulappan , Luong John H. T. , Gedanken Aharon
Published: January 2025

Amidst the pervasive threat of bacterial afflictions, the imperative for advanced antibiofilm surfaces with robust antimicrobial efficacy looms large. This study unveils a sophisticated ultrasonic synthesis method for cellulose nanocrystals (CNCs, 10–20 nm in diameter and 300–900 nm in length) and their subsequent application as coatings on flexible substrates, namely cotton (CC-1) and membrane (CM-1). The cellulose nanocrystals showed excellent water repellency with a water contact angle as high as 148° on the membrane. Noteworthy attributes of CNC-coated substrates include augmented reactive oxygen species (ROS) generation, heightened surface hydrophobicity, and comprehensive suppression of both drug-sensitive (MDR E. coli and MRSA) and susceptible (E. coli and S. aureus) planktonic and biofilm bacterial proliferation. In contrast, the uncoated substrates display 100% bacterial growth for the above bacteria. Empirical data corroborate the pronounced biofilm mass reduction capabilities of CNC-coated substrates across all tested bacterial strains. Elucidation of underlying mechanisms implicates ROS generation and electrostatic repulsion between CNCs and bacterial membranes in the disruption of mature biofilms. Hydroxyl radicals, superoxide, and hydrogen peroxide possess formidable reactivity, capable of disrupting essential biomolecules such as DNA, proteins, and lipids. The engineered CNC-coated substrates platform evinces considerable promise in the realm of infectious disease management, offering a cogent blueprint for the development of novel antimicrobial matrices adept at combating bacterial infections with efficacy and precision.
Effect of Crystallinity on the Printability of Poly(ethylene Terephthalate)/Poly(butylene Terephthalate) Blends
Authors: Aliberti Francesca , Oliviero Maria , Longo Raffaele , Guadagno Liberata , Sorrentino Andrea
Published: January 2025

This study explores the impact of blending polyethylene terephthalate (PET) with polybutylene terephthalate (PBT) on the thermal, structural, and mechanical properties of 3D-printed materials. Comprehensive analyses, including Fourier-transform infrared spectroscopy (FT-IR), thermogravimetric analysis (TGA), X-ray diffraction (XRD), differential scanning calorimetry (DSC), and mechanical testing, were conducted to assess the influence of blend composition. FT-IR confirmed that PET and PBT blend physically without transesterification, while TGA showed enhanced thermal stability with increasing PET content. XRD revealed that PET and PBT crystallize separately, with the crystallinity decreasing sharply for blends with more than 50% PET. The DSC results indicated that PET effectively slows down the crystallization kinetics of PBT, promoting cold crystallization. Mechanical tests demonstrated that the elastic modulus remains relatively unchanged, but the strain at break decreases with a higher PET content, indicating increased stiffness and reduced ductility. Overall, incorporating PET into PBT improves 3D-printability and dimensional stability, reducing warpage and enhancing print precision, making these blends advantageous for 3D-printing applications.