bluuebunny/crossref_metadata_2025 huggingface dataset:
Single-Stage Step-Down Power Factor Corrector without Full-Bridge Rectifier
Authors: Hwu Kuo-Ing , Shieh Jenn-Jong , He Yu-Ping
Published: April 2024

In this paper, a single-stage step-down power factor corrector without a full-bridge rectifier is developed, which is designed to operate in discontinuous conduction mode (DCM). In terms of control, the DCM has the advantages of simple control and easy realization, no slope compensation, zero current switching, and no diode reverse current. By sampling the output voltage and using the voltage-follower control to generate the necessary control force to drive the power switch, not only can the output voltage be stabilized at the desired value, but also the input current can be, as much as possible, in the form of a sinusoidal waveform and can follow the phase of the input voltage. Moreover, the harmonic distortion meets the requirements of the IEC6100-3-2 Class D harmonics standard, and, thus, the proposed rectifier is appropriate for the computer, computer monitor, and television receiver. Eventually, by means of mathematical deductions, simulations by PSIM version 9.1, and experimental results, the feasibility and effectiveness of the proposed circuit can be verified.
Cylindrical Steel Tanks Subjected to Long-Duration and High-Pressure Triangular Blast Load: Current Practice and a Numerical Case Study
Authors: Rosin Julia , Stocchi Alessandro , Bruckhaus Norman , Heyner Johanna , Weidner Philipp , Waas Till
Published: April 2024

This paper presents an investigation into the design of ammonia tanks for long-duration and high-pressure blast loads. The focus is on cylindrical steel tanks that apply as outer pressure-tight containers for double-walled tanks storing refrigerated liquefied gases. Based on limited empirical data, it is known in the tank industry that these tanks can withstand an explosion pressure up to a peak overpressure of approximately 10 kPa and 100 ms positive load duration. However, there is a growing need to design tanks for higher peak overpressures in order to establish a higher safety standard and accommodate unforeseen future requirements. This paper explores the concept of adapting established steel tank designs to handle high-pressure and long-duration overpressure due to blast events. Numerical analysis is conducted on a representative steel tank geometry subjected to a triangular blast load of 30 kPa with a 300 ms positive load duration. Various load application and calculation options are analyzed numerically. Considering the challenging nature of analyzing tank structures under blast load, the paper addresses controversial aspects discussed in the literature and presents a suitable analysis concept for a deflagration blast scenario for cylindrical tanks. The results provide insights into the expected structural behavior of the tank under high-pressure and long-duration overpressure. The main finding is that the calculation method developed in this study demonstrates the feasibility of utilizing steel tanks in scenarios involving long-duration and high-pressure blast loads. Furthermore, the paper provides recommendations to guide future studies in this area. The findings have implications for the design and construction of tanks in critical infrastructure and offer valuable insights for engineers and researchers in this field, improving safety standards and ensuring adaptability to future utilization concepts.
Design and Development of Complex-Order PI-PD Controllers: Case Studies on Pressure and Flow Process Control
Authors: Bin Roslan Muhammad Najmi , Bingi Kishore , Devan P. Arun Mozhi , Ibrahim Rosdiazli
Published: April 2024

This article examines the performance of the proposed complex-order, conventional and fractional-order controllers for process automation and control in process plants. The controllers are compared regarding disturbance rejection and set-point tracking, considering variables such as response time, robustness to uncertainty, and steady-state error. The study shows that a complex PI-PD controller has better accuracy, faster response time, and better noise rejection. Still, implementation is challenging due to increased complexity and processing requirements. In contrast, a standard PI-PD controller is a known solution but may have problems with accuracy and robustness. Fractional-order controllers based on fractional computations have the potential to improve control accuracy and robustness of non-linear and time-varying systems. Experimental insights and real-world case studies are used to highlight the strengths and weaknesses of each controller. The findings provide valuable insights into the strengths and weaknesses of complex-order and fractional-order controllers and help to select the appropriate controller for specific process plant requirements. Future perspectives on controller design and performance optimization are detailed, identifying the potential benefits of using complex and fractional-order controllers in process plants.
Characterization of Microbials in the Lung Induced by Allergenic Platanus Pollen Protein (Pla a3) and Ambient Fine Particulate Matter
Authors: Liu Jin , Lu Senlin , Hou Guoqing , Hu Wenwen , Zhao Jiumei , Zhang Wei , Liu Xinchun , Ebere Enyoh Christian , Wang Weiqian , Wang Qingyue
Published: April 2024

Ambient pollen proteins play key roles in the incidence of allergenic respiratory health, and numerous reports have focused on respiratory diseases caused by air pollutants. However, there is still a lack of understanding of the specific mechanisms underlying the involvement of microbiota in the respiratory tracts and effects induced by air pollutants. Therefore, an allergenic animal model was established to investigate the characterization of microbials in the lung induced by allergenic Platanus pollen protein (Pla a3) and ambient fine particulate matter. Our data showed that the mice exhibited strong immune and inflammatory responses after being exposed to PMs and Pla a3 protein. This included increased levels of immunoglobulins IgG and IgE, as well as elevated levels of cytokines TNF-α, IFN-γ, IL-4, and IL-13. Furthermore, the amounts of pathogenic bacteria, such as Desulfobacterota, Enterococcus, Ferruginibacter, and Pseudoxanthomonas, in the lung microbiota of the Pla a3 exposure group increased significantly. Correlation analysis revealed a strong association between specific lung bacteria and alterations in cytokines from the lung samples. Probiotic bacteria, Deferribacterota and Bifidobacterium, was associated with changes in the level of IgG and IgE. However, pathogenic bacteria, like Prevotella and Fusobacterium, were linked with the cytokines IL-4 and TNF-α.
Stabilization of the Interface between a PEO-Based Lithium Solid Polymer Electrolyte and a 4-Volt Class Cathode, LiCoO2, by the Addition of LiPF6 as a Lithium Salt
Authors: Taminato Sou , Tsuka Akino , Sobue Kento , Mori Daisuke , Takeda Yasuo , Yamamoto Osamu , Imanishi Nobuyuki
Published: April 2024

Here, the time dependence of the interfacial resistance for Li/polyethylene oxide (PEO)-Li(CF3SO2)2N (LiTFSI)-LiPF6/LiCoO2 cells was measured to investigate the stabilization effect of LiPF6 on the interface between a solid polymer electrolyte (SPE) and a 4-volt class cathode, LiCoO2. Impedance measurements under the applied potentials between 4.1 V and 4.4 V vs. Li/Li+ indicated that the addition of LiPF6 to LiTFSI was effective in improving the stability at high potentials such as 4.4 V vs. Li/Li+. In contrast, the resistance of the non-doped PEO-LiTFSI/LiCoO2 interface increased with time under the lower potential of 4.1 V vs. Li/Li+. Fairly good cycle performance was obtained for the LiPF6-doped cell, even at a cut-off voltage of 4.5 V vs. Li/Li+.
Dimensionality Matters: Exploiting UV-Photopatterned 2D and Two-Photon-Printed 2.5D Contact Guidance Cues to Control Corneal Fibroblast Behavior and Collagen Deposition
Authors: van der Putten Cas , Sahin Gozde , Grant Rhiannon , D’Urso Mirko , Giselbrecht Stefan , Bouten Carlijn V. C. , Kurniawan Nicholas A.
Published: April 2024

In the event of disease or injury, restoration of the native organization of cells and extracellular matrix is crucial for regaining tissue functionality. In the cornea, a highly organized collagenous tissue, keratocytes can align along the anisotropy of the physical microenvironment, providing a blueprint for guiding the organization of the collagenous matrix. Inspired by this physiological process, anisotropic contact guidance cues have been employed to steer the alignment of keratocytes as a first step to engineer in vitro cornea-like tissues. Despite promising results, two major hurdles must still be overcome to advance the field. First, there is an enormous design space to be explored in optimizing cellular contact guidance in three dimensions. Second, the role of contact guidance cues in directing the long-term deposition and organization of extracellular matrix proteins remains unknown. To address these challenges, here we combined two microengineering strategies—UV-based protein patterning (2D) and two-photon polymerization of topographies (2.5D)—to create a library of anisotropic contact guidance cues with systematically varying height (H, 0 µm ≤ H ≤ 20 µm) and width (W, 5 µm ≤ W ≤ 100 µm). With this unique approach, we found that, in the short term (24 h), the orientation and morphology of primary human fibroblastic keratocytes were critically determined not only by the pattern width, but also by the height of the contact guidance cues. Upon extended 7-day cultures, keratocytes were shown to produce a dense, fibrous collagen network along the direction of the contact guidance cues. Moreover, increasing the heights also increased the aligned fraction of deposited collagen and the contact guidance response of cells, all whilst the cells maintained the fibroblastic keratocyte phenotype. Our study thus reveals the importance of dimensionality of the physical microenvironment in steering both cellular organization and the formation of aligned, collagenous tissues.
A Novel Bio-Purification Process Employing an Engineered E. coli Strain for Downstream Processing of Lactic Acid Solutions from the Fermentation of Agro-Industrial by-Products
Authors: Nastouli Alexandra , Moschona Alexandra , Bizirtsakis Panagiotis A. , Sweeney Joseph , Angelidaki Irini , Harasek Michael , Karabelas Anastasios J. , Patsios Sotiris I.
Published: April 2024

This study aims to integrate a novel bio-purification process employing an engineered E. coli strain in the downstream processing of lactic acid (LA) fermentation broths from low-cost renewable biological feedstocks. Fermentation broth of candy waste and digestate mixture was used as a real biological feedstock. An engineered E. coli strain that selectively catabolize impurities without catabolizing LA was initially adapted on the biological feedstock, followed by shake flask experiments to prove the bio-purification concept. Scale-up and validation in a bench-scale bioreactor followed, before developing a semi-continuous membrane bioreactor (MBR) bio-purification process. The MBR bio-purification was assessed with biological feedstocks which simulated ultrafiltration or nanofiltration permeates. Incomplete removal of impurities and increased fouling was observed in the case of the ultrafiltration permeate. Contrarily, the nanofiltration permeate was successfully treated with MBR bio-purification, since low membrane fouling, 100% maltose and acetic acid removal, and no LA catabolism was achieved. MBR bio-purification as a post-treatment step in the downstream processing of LA was demonstrated as a promising technology for increasing the purity of LA solutions.
Predictive Refined Computational Modeling of ACL Tear Injury Patterns
Authors: Sharabi Mirit , Agron Raz , Dolev Amir , Haj-Ali Rami , Yassin Mustafa
Published: April 2024

Anterior cruciate ligament (ACL) ruptures are prevalent knee injuries, with approximately 200,000 ruptures annually, and treatment costs exceed USD two billion in the United States alone. Typically, the initial detection of ACL tears and anterior tibial laxity (ATL) involves manual assessments like the Lachman test, which examines anterior knee laxity. Partial ACL tears can go unnoticed if they minimally affect knee laxity; however, they will progress to a complete ACL tear requiring surgical treatment. In this study, a computational finite element model (FEM) of the knee joint was generated to investigate the effect of partial ACL tears under the Lachman test (GNRB® testing system) boundary conditions. The ACL was modeled as a hyperelastic composite structure with a refined representation of collagen bundles. Five different tear types (I–V), classified by location and size, were modeled to predict the relationship between tear size, location, and anterior tibial translation (ATT). The results demonstrated different levels of ATT that could not be manually detected. Type I tears demonstrated an almost linear increase in ATT, with the growth in tear size ranging from 3.7 mm to 4.2 mm, from 25% to 85%, respectively. Type II partial tears showed a less linear incline in ATT (3.85, 4.1, and 4.75 mm for 25%, 55%, and 85% partial tears, respectively). Types III, IV, and V maintained a nonlinear trend, with ATTs of 3.85 mm, 4.2 mm, and 4.95 mm for Type III, 3.85 mm, 4.25 mm, and 5.1 mm for Type IV, and 3.6 mm, 4.25 mm, and 5.3 mm for Type V, for 25%, 55%, and 85% partial tears, respectively. Therefore, for small tears (25%), knee stability was most affected when the tears were located around the center of the ligament. For moderate tears (55%), the effect on knee stability was the greatest for tears at the proximal half of the ACL. However, severe tears (85%) demonstrated considerable growth in knee instability from the distal to the proximal ends of the tissue, with a substantial increase in knee instability around the insertion sites. The proposed model can enhance the characterization of partial ACL tears, leading to more accurate preliminary diagnoses. It can aid in developing new techniques for repairing partially torn ACLs, potentially preventing more severe injuries.
Cellular Response of Adapted and Non-Adapted Tetrahymena thermophila Strains to Europium Eu(III) Compounds
Authors: Alonso Patricia , Blas Javier , Amaro Francisco , de Francisco Patricia , Martín-González Ana , Gutiérrez Juan Carlos
Published: April 2024

Europium is one of the most reactive lanthanides and humans use it in many different applications, but we still know little about its potential toxicity and cellular response to its exposure. Two strains of the eukaryotic microorganism model Tetrahymena thermophila were adapted to high concentrations of two Eu(III) compounds (EuCl3 or Eu2O3) and compared to a control strain and cultures treated with both compounds. In this ciliate, EuCl3 is more toxic than Eu2O3. LC50 values show that this microorganism is more resistant to these Eu(III) compounds than other microorganisms. Oxidative stress originated mainly by Eu2O3 is minimized by overexpression of genes encoding important antioxidant enzymes. The overexpression of metallothionein genes under treatment with Eu(III) compounds supports the possibility that this lanthanide may interact with the -SH groups of the cysteine residues from metallothioneins and/or displace essential cations of these proteins during their homeostatic function. Both lipid metabolism (lipid droplets fusing with europium-containing vacuoles) and autophagy are involved in the cellular response to europium stress. Bioaccumulation, together with a possible biomineralization to europium phosphate, seems to be the main mechanism of Eu(III) detoxification in these cells.
The Use of Extracellular Vesicles in Achilles Tendon Repair: A Systematic Review
Authors: Kasula Varun , Padala Vikram , Gupta Nithin , Doyle David , Bagheri Kian , Anastasio Albert , Adams Samuel Bruce
Published: April 2024

Achilles tendon (AT) pathologies are common musculoskeletal conditions that can significantly impair function. Despite various traditional treatments, recovery is often slow and may not restore full functionality. The use of extracellular vesicles (EVs) has emerged as a promising therapeutic option due to their role in cell signaling and tissue regeneration. This systematic review aims to consolidate current in vivo animal study findings on the therapeutic effects of EVs on AT injuries. An extensive literature search was conducted using the PubMed, Scopus, and Embase databases for in vivo animal studies examining the effects of EVs on AT pathologies. The extracted variables included but were not limited to the study design, type of EVs used, administration methods, efficacy of treatment, and proposed therapeutic mechanisms. After screening, 18 studies comprising 800 subjects were included. All but one study reported that EVs augmented wound healing processes in the AT. The most proposed mechanisms through which this occurred were gene regulation of the extracellular matrix (ECM), the enhancement of macrophage polarization, and the delivery of therapeutic microRNAs to the injury site. Further research is warranted to not only explore the therapeutic potential of EVs in the context of AT pathologies, but also to establish protocols for their clinical application.
Special Prey, Special Glue: NMR Spectroscopy on Aggregate Glue Components of Moth-Specialist Spiders, Cyrtarachninae
Authors: VanDyck Max W. , Long John H. , Baker Richard H. , Hayashi Cheryl Y. , Diaz Candido
Published: April 2024

Orb-weaver spiders produce upwards of seven different types of silk, each with unique material properties. We focus on the adhesive within orb-weaving spider webs, aggregate glue silk. These droplets are composed of three main components: water, glycoproteins, and a wide range of low molecular mass compounds (LMMCs). These LMMCs are known to play a crucial role in maintaining the material properties of the glycoproteins, aid in water absorption from the environment, and increase surface adhesion. Orb-weavers within the Cyrtarachninae subfamily are moth specialists and have evolved glue droplets with novel material properties. This study investigated the biochemical composition and diversity of the LMMCs present in the aggregate glue of eight moth-specialist species and compared them with five generalist orb-weavers using nuclear magnetic resonance (NMR) spectroscopy. We hypothesized that the novel drying ability of moth-specialist glue was accompanied by novel LMMCs and lower overall percentages by silk weight of LMMCs. We measured no difference in LMMC weight by the type of prey specialization, but observed novel compositions in the glue of all eight moth-catching species. Further, we quantified the presence of a previously reported but unidentified compound that appears in the glue of all moth specialists. These silks can provide insight into the functions of bioadhesives and inform our own synthetic adhesives.
The Impact of Fatigue on Sleep and Other Non-Motor Symptoms in Parkinson’s Disease
Authors: Diaconu Stefania , Monescu Vlad , Filip Rafaela , Marian Laura , Kakucs Cristian , Murasan Iulia , Chaudhuri K. Ray , Jianu Dragos Catalin , Falup-Pecurariu Cristian , Opritoiu Bianca
Published: April 2024

Fatigue is a common non-motor symptom in Parkinson’s disease (PD), but even so, it may still be underdiagnosed or misdiagnosed in current practice due to its non-specific manifestations. The aims of this study were to investigate the prevalence of fatigue in PD patients compared to healthy controls and to identify the main characteristics and associations of fatigue with other non-motor symptoms and the impact of fatigue on sleep disturbances in Parkinson’s disease. Materials and methods: case–control study in which 131 PD patients and 131 age- and sex-matched controls were enrolled. Main characteristics of fatigue, sleep, and other non-motor symptoms were assessed using specific validated questionnaires. Results: According to the Chalder fatigue scale, fatigue is more prevalent in PD patients (38.16%) compared to healthy controls (26.71%). Fatigue was identified in 46.54% of the PD patients using the Parkinson’s Fatigue Scale (PFS-16). PD patients with fatigue presented a worse motor status, more sleep disturbances (insomnia, daytime sleepiness), a broader spectrum of non-motor symptoms (pain, anxiety, urinary disturbances), worse cognitive performances, a lower level of happiness, and worse quality of life compared to PD patients without fatigue. Conclusion: Fatigue is a common symptom of PD and needs to be assessed, considering its consequences on quality of life. Sleep disturbances have a great influence over fatigue in PD patients.
Acupuncture Extended the Thrombolysis Window by Suppressing Blood–Brain Barrier Disruption and Regulating Autophagy–Apoptosis Balance after Ischemic Stroke
Authors: Zhang Zhihui , Lu Tianliang , Li Shanshan , Zhao Ruyu , Li Honglei , Zhang Xinchang , Li Yiyang , Xia Yawen , Ni Guangxia
Published: April 2024

Background: Ischemic stroke (IS) is one of the leading causes of death and disability worldwide. The narrow therapeutic window (within 4.5 h) and severe hemorrhagic potential limits therapeutic efficacy of recombinant tissue type plasminogen activator (rt-PA) intravenous thrombolysis for patients. Xingnao Kaiqiao (XNKQ) acupuncture is an integral part of traditional Chinese medicine, specifically designed to address acute ischemic stroke by targeting key acupoints such as Shuigou (GV26) and Neiguan (PC6). In this study, we explored the therapeutic potential of XNKQ acupuncture in extending the time window for thrombolysis and interrogated the molecular mechanisms responsible for this effect. Methods: The effect of extending the thrombolysis window by acupuncture was evaluated via TTC staining, neuronal score evaluation, hemorrhagic transformation assay, and H&E staining. RNA sequencing (RNA-seq) technology was performed to identify the therapeutic targets and intervention mechanisms of acupuncture. Evans blue staining and transmission electron microscopy were used to assess blood–brain barrier (BBB) integrity. Immunofluorescence staining and co-immunoprecipitation were performed to evaluate the level of autophagy and apoptosis and validate their interactions with BBB endothelial cells. Results: Acupuncture alleviated infarction and neurological deficits and extended the thrombolysis window to 6 h. The RNA-seq revealed 16 potential therapeutic predictors for acupuncture intervention, which related to suppressing inflammation and restoring the function of BBB and blood vessels. Furthermore, acupuncture suppressed BBB leakage and preserved tight junction protein expression. The protective effect was associated with regulation of the autophagy–apoptosis balance in BBB endothelial cells. Acupuncture intervention dissociated the Beclin1/Bcl-2 complex, thereby promoting autophagy and reducing apoptosis. Conclusion: XNKQ acupuncture could serve as an adjunctive therapy for rt-PA thrombolysis, aiming to extend the therapeutic time window and mitigate ischemia–reperfusion injury. Acupuncture suppressed BBB disruption by regulating the autophagy–apoptosis balance, which in turn extended the therapeutic window of rt-PA in IS. These findings provide a rationale for further exploration of acupuncture as a complementary candidate co-administered with rt-PA.
Energy Performance and Comfort Analysis of Three Glazing Materials with Distinct Thermochromic Responses as Roller Shade Alternative in Cooling- and Heating-Dominated Climates
Authors: Tennakoon Thilhara , Chan Yin-Hoi , Chan Ka-Chung , Wu Chili , Chao Christopher Yu-Hang , Fu Sau-Chung
Published: April 2024

Thermochromic (TC) smart windows are a leading passive building design strategy. Vanadium dioxide (VO2), hydrogel and TC-Perovskite glazing, which constitute the main categories of TC materials, modulate different wavelength regions. Although numerous studies have reported on these TC glazings’ energy-saving potential individually, there is a lack of data comparing their energy efficiencies. Moreover, their suitability as an alternative to dynamic solar shading mechanisms remains unexplored. Using building energy simulation, this study found that a hydrogel glazing with broadband thermochromism can save more energy (22–24% savings on average) than opaque roller shades (19–20%) in a typical office in both New York and Hong Kong. VO2 glazing performed comparably to translucent roller shades (14–16% savings), except when used in poorly daylit conditions. TC-Perovskite was a poor replacement for roller shades (~2% savings). The window-to-wall ratio (WWR) that allowed both energy savings and optimal natural light penetration was also identified for each glazing. Hydrogel glazing demonstrated both energy and daylight efficiency in Hong Kong’s cooling-dominated climate when used in 40–50% WWR configurations. In New York’s colder conditions, VO2 glazing did so for higher WWRs (50–70%). Roller shades could also achieve simultaneous energy savings and visual comfort, but only for highly glazed facades (up to 80%).
The Role of β3-Adrenergic Receptors in Cold-Induced Beige Adipocyte Production in Pigs
Authors: Yang Shuo , Ma Hong , Wang Liang , Wang Fang , Xia Jiqiao , Liu Dongyu , Mu Linlin , Yang Xiuqin , Liu Di
Published: April 2024

After exposure to cold stress, animals enhance the production of beige adipocytes and expedite thermogenesis, leading to improved metabolic health. Although brown adipose tissue in rodents is primarily induced by β3-adrenergic receptor (ADRB3) stimulation, the activation of major β-adrenergic receptors (ADRBs) in pigs has been a topic of debate. To address this, we developed overexpression vectors for ADRB1, ADRB2, and ADRB3 and silenced the expression of these receptors to observe their effects on the adipogenic differentiation stages of porcine preadipocytes. Our investigation revealed that cold stress triggers the transformation of subcutaneous white adipose tissue to beige adipose tissue in pigs by modulating adrenergic receptor levels. Meanwhile, we found that ADRB3 promotes the transformation of white adipocytes into beige adipocytes. Notably, ADRB3 enhances the expression of beige adipose tissue marker genes, consequently influencing cellular respiration and metabolism by regulating lipolysis and mitochondrial expression. Therefore, ADRB3 may serve as a pivotal gene in animal husbandry and contribute to the improvement of cold intolerance in piglets.
Genomic Engineering of Oral Keratinocytes to Establish In Vitro Oral Potentially Malignant Disease Models as a Platform for Treatment Investigation
Authors: Wils Leon J. , Buijze Marijke , Stigter-van Walsum Marijke , Brink Arjen , van Kempen Britt E. , Peferoen Laura , Brouns Elisabeth R. , de Visscher Jan G. A. M. , van der Meij Erik H. , Bloemena Elisabeth , Poell Jos B. , Brakenhoff Ruud H.
Published: April 2024

Precancerous cells in the oral cavity may appear as oral potentially malignant disorders, but they may also present as dysplasia without visual manifestation in tumor-adjacent tissue. As it is currently not possible to prevent the malignant transformation of these oral precancers, new treatments are urgently awaited. Here, we generated precancer culture models using a previously established method for the generation of oral keratinocyte cultures and incorporated CRISPR/Cas9 editing. The generated cell lines were used to investigate the efficacy of a set of small molecule inhibitors. Tumor-adjacent mucosa and oral leukoplakia biopsies were cultured and genetically characterized. Mutations were introduced in CDKN2A and TP53 using CRISPR/Cas9 and combined with the ectopic activation of telomerase to generate cell lines with prolonged proliferation. The method was tested in normal oral keratinocytes and tumor-adjacent biopsies and subsequently applied to a large set of oral leukoplakia biopsies. Finally, a subset of the immortalized cell lines was used to assess the efficacy of a set of small molecule inhibitors. Culturing and genomic engineering was highly efficient for normal and tumor-adjacent oral keratinocytes, but success rates in oral leukoplakia were remarkably low. Knock-out of CDKN2A in combination with either the activation of telomerase or knock-out of TP53 seemed a prerequisite for immortalization. Prolonged culturing was accompanied by additional genetic aberrations in these cultures. The generated cell lines were more sensitive than normal keratinocytes to small molecule inhibitors of previously identified targets. In conclusion, while very effective for normal keratinocytes and tumor-adjacent biopsies, the success rate of oral leukoplakia cell culturing methods was very low. Genomic engineering enabled the prolonged culturing of OL-derived keratinocytes but was associated with acquired genetic changes. Further studies are required to assess to what extent the immortalized cultures faithfully represent characteristics of the cells in vivo.
Nuclear Phospholipids and Signaling: An Update of the Story
Authors: Casalin Irene , Ceneri Eleonora , Ratti Stefano , Manzoli Lucia , Cocco Lucio , Follo Matilde Y.
Published: April 2024

In the last three decades, the presence of phospholipids in the nucleus has been shown and thoroughly investigated. A considerable amount of interest has been raised about nuclear inositol lipids, mainly because of their role in signaling acting. Here, we review the main issues of nuclear phospholipid localization and the role of nuclear inositol lipids and their related enzymes in cellular signaling, both in physiological and pathological conditions.
Treatment Effects of Introducing the Neurosequential Model of Therapeutics in a Norwegian Residential Treatment Facility for Children Aged 7–13
Authors: Johannessen Kaja Næss , Bakken Ann-Karin , Hambrick Erin P. , Solbakken Ole André
Published: April 2024

This study investigates the impact of the Neurosequential Model of Therapeutics (NMT) in child and adolescent psychiatric care, addressing a gap in current clinical methodologies that tend to focus on single problems rather than the interconnected nature of many real-life mental health issues. The study was conducted in a residential setting over an extended period, including children aged 7–13, to observe the effects of implementing NMT. The children presented with complex symptoms and multiple diagnoses. The methods incorporated the NMT approach, emphasizing individualized treatment plans based on each child’s unique brain development, and aimed at addressing multiple, interconnected problems simultaneously. Results from multilevel model analyses of behavioral difficulties, measured using the Child Behavior Checklist (CBCL), revealed substantial improvements in treatment effectiveness post-NMT implementation. Despite the limitations, such as a non-randomized participant selection and limited sample size, the findings strongly suggest that NMT enhances care effectiveness in real-world clinical settings, particularly for children with complex mental health issues. The study concludes that relationally oriented milieu therapy, and specifically the NMT approach, holds great promise for advancing pediatric psychiatric care, advocating for its broader application and further research to refine and substantiate its efficacy.
The Significance of Thyroid Hormone Receptors in Breast Cancer: A Hypothesis-Generating Narrative Review
Authors: Quan Trinity , Cockburn Jessica , Dhesy-Thind Sukhbinder , Bane Anita , Leong Hon , Geleff Christopher , Devion Catherine , Ajel Noor , Jerzak Katarzyna J.
Published: April 2024

Background: Breast cancer (BC) is frequently diagnosed among Canadian women. While targeted therapies are available for most BC patients; treatment resistance is common and novel therapeutic targets are of interest. Thyroid hormones (TH) bound to thyroid hormone receptors (THR) influence cell proliferation and differentiation; they are also involved in the growth and development of normal breast tissue. Evidence suggests that THRβ is a tumor suppressor in various solid tumors. Purpose: This narrative review discusses retrospective studies regarding the clinical relevance of THRβ as a potential prognostic biomarker and therapeutic target in BC. Methods: We consulted with an information specialist to develop a search strategy to find all literature related to THRα expression as a potential prognostic and therapeutic biomarker in breast cancer. The primary search was developed for Medline and translated to Embase. The searches were conducted on the Ovid platform on 18 August 2023. Results: Across seven retrospective studies identified, several have shown an association between higher THRβ1 expression with a lower risk of BC recurrence and with longer overall survival. Conclusions: Some evidence suggests that THRβ expression is associated with a lower risk of BC recurrence and death. Validation of THRβ as an independent prognostic biomarker and possible predictive biomarker of response to endocrine therapy and/or chemotherapy is of interest. Given that THRβ is upstream of the AKT/PI3K pathway, its potential as a predictive biomarker of response to AKT inhibitors and/or PI3K inhibitors may also be of value. Finally, the potential re-purposing of THRβ agonists as anti-cancer agents warrants investigation.
12-Month Trajectories of Health-Related Quality of Life Following Hospitalization in German Cancer Centers—A Secondary Data Analysis
Authors: Eichler Martin , Hönig Klaus , Bergelt Corinna , Faller Hermann , Maatouk Imad , Hornemann Beate , Stein Barbara , Teufel Martin , Goerling Ute , Erim Yesim , Geiser Franziska , Niecke Alexander , Senf Bianca , Weis Joachim
Published: April 2024

Patient-reported outcomes (PROs) offer a diverse array of potential applications within medical research and clinical practice. In comparative research, they can serve as tools for delineating the trajectories of health-related quality of life (HRQoL) across various cancer types. We undertook a secondary data analysis of a cohort of 1498 hospitalized cancer patients from 13 German cancer centers. We assessed the Physical and Mental Component Scores (PCS and MCS) of the 12-Item Short-Form Health Survey at baseline (t0), 6 (t1), and 12 months (t2), using multivariable generalized linear regression models. At baseline, the mean PCS and MCS values for all cancer patients were 37.1 and 44.3 points, respectively. We observed a significant improvement in PCS at t2 and in MCS at t1. The most substantial and significant improvements were noted among patients with gynecological cancers. We found a number of significant differences between cancer types at baseline, t1, and t2, with skin cancer patients performing best across all time points and lung cancer patients performing the worst. MCS trajectories showed less pronounced changes and differences between cancer types. Comparative analyses of HRQoL scores across different cancer types may serve as a valuable tool for enhancing health literacy, both among the general public and among cancer patients themselves.
Intratarsal Keratinous Cyst Clinically Misdiagnosed as a Chalazion
Authors: Cunha John Lennon Silva , Andrade Clenia E. S. , da Cunha Filho Fernando A. P. , da Paz Alexandre R. , Gordón-Núñez Manuel A. , Alves Pollianna M. , Nonaka Cassiano F. W.
Published: April 2024

The intratarsal keratinous cyst (IKC) is a recently described entity, often clinically misdiagnosed as a chalazion. We report a case of a 61-year-old male patient with a chief complaint of a small lesion on the upper eyelid that evolved over six months. On physical examination, an asymptomatic, firm nodule was identified on the left upper eyelid. The patient reported no history of trauma. A provisional diagnosis of chalazion was established, and an excisional biopsy was performed. Histopathologically, the lesion was lined with a stratified squamous epithelium, with a corrugated epithelial surface showing abrupt keratinization without keratohyalin granules, and compact keratinous-appearing material in the cystic lumen. The diagnosis was IKC. No signs of recurrence were observed after one year of follow-up. It is essential to accurately diagnose IKC and distinguish it from chalazion and epidermal inclusion cysts, because IKC requires complete surgical excision and can exhibit multiple recurrences if not properly removed.
Impact Analysis of Time Synchronization Error in Airborne Target Tracking Using a Heterogeneous Sensor Network
Authors: Lee Seokwon , Yuan Zongjian , Petrunin Ivan , Shin Hyosang
Published: April 2024

This paper investigates the influence of time synchronization on sensor fusion and target tracking. As a benchmark, we design a target tracking system based on track-to-track fusion architecture. Heterogeneous sensors detect targets and transmit measurements through a communication network, while local tracking and track fusion are performed in the fusion center to integrate measurements from these sensors into a fused track. The time synchronization error is mathematically modeled, and local time is biased from the reference clock during the holdover phase. The influence of the time synchronization error on target tracking system components such as local association, filtering, and track fusion is discussed. The results demonstrate that an increase in the time synchronization error leads to deteriorating association and filtering performance. In addition, the results of the simulation study validate the impact of the time synchronization error on the sensor network.
Planetary Energy Flow and Entropy Production Rate by Earth from 2002 to 2023
Authors: Thimsen Elijah
Published: April 2024

In this work, satellite data from the Clouds and Earth’s Radiant Energy System (CERES) and Moderate Resolution Imaging Spectroradiometer (MODIS) instruments are analyzed to determine how the global absorbed sunlight and global entropy production rates have changed from 2002 to 2023. The data is used to test hypotheses derived from the Maximum Power Principle (MPP) and Maximum Entropy Production Principle (MEP) about the evolution of Earth’s surface and atmosphere. The results indicate that both the rate of absorbed sunlight and global entropy production have increased over the last 20 years, which is consistent with the predictions of both hypotheses. Given the acceptance of the MPP or MEP, some peripheral extensions and nuances are discussed.
School Leadership and Management in Sindh Pakistan: Examining Headteachers’ Evolving Roles, Contemporary Challenges, and Adaptive Strategies
Authors: Shah Dhani Bux , Gurr David , Drysdale Lawrie
Published: April 2024

This research investigates headteachers’ leadership and management roles and challenges in leading government secondary schools in Sindh, Pakistan. Employing a qualitative hermeneutic phenomenology methodology, forty semi-structured interviews were conducted with twenty headteachers. The thematic analysis of the data explored that headteachers were performing several academic and administrative-focused responsibilities, although their involvement was more administrative than academic and entailed more management than leadership. However, headteachers faced enormous challenges in leading and managing their schools. Some critical challenges were a lack of basics in terms of clean water, reliable electricity, and sufficient school furniture. Others included a lack of funds, a shortage of teachers, managing professionally weak teachers, teacher union issues and a lack of authority. Nevertheless, some headteachers were making admirable efforts to address these challenges. This research may open a window to understanding the roles and challenges of headteachers and inform policymakers of how-to better support headteachers in leading their schools successfully in the 21st century.
Assessment of Spent Nuclear Fuel in Ukrainian Storage System: Inventory and Performance
Authors: Dolin Viktor , Lo Frano Rosa , Cancemi Salvatore Angelo
Published: April 2024

It is of meaningful importance to evaluate the performance of all the nuclear facilities, and particularly those part of such buildings where spent nuclear fuel (SNF) is stored to assess what kinds of consequences are anomalous/abnormal or to determine what types of accident events may occur. In this preliminary study, the strategies adopted for the management of SNF, and the risk related to them are discussed. The aim of this study is to evaluate the total radioactivity inventory characterising Ukrainian nuclear facilities, including storage facilities. The dataset used to calculate the total activity associated with nuclear fuel is provided and discussed. For the evaluation, it is considered that a SNF pool in VVER-1000 is designed to store 687 fuel assemblies, and 670 are in VVER-440. When it is half full, which is the case for 15 Ukrainian units, it will store about 2200 tU containing up to 1·1019 Bq of 137Cs, 7·1018 Bq of 90Sr, and 1·1019 Bq of TUE. This study focuses particularly on the total activity of the SNF stored at the Zaporozhye plant, the biggest nuclear plant in Europe, and the risk posed by the potential loss that cooling the plant could incur because of pond water level variation. The results of the analysis of the Zaporozhye NPP behaviour suggest that the water flow rate which keeps the SNF pool temperature constant is about 200,000 m3·day−1. Therefore, the water level in the pond should not be lower than 1.5–2 m; otherwise, the plant will need an additional source of water of more than 200,000 m3 per day to guarantee safe storage of SNF.
Experimental Identification of a Coupled-Circuit Model for the Digital Twin of a Wound-Rotor Induction Machine
Authors: Aboubi Fatma Zohra , Maïga Abdrahamane , Cros Jérôme , Kamwa Innocent
Published: April 2024

The development of monitoring and diagnostic methods for electrical machines requires the use of transient models capable of operating in real time and producing signal signatures with high precision. In this context, coupled-circuit models offer numerous advantages due to their speed of execution and accuracy. They have been successfully employed to create real-time digital twins of electrical machines. The main challenge of this modeling method lies in the preparation of the model, which involves numerous preliminary calculations and takes time to identify all its parameters. This is particularly due to the variation in inductances based on the rotor position. To determine these inductance values with great precision, the classical approach involves using finite-element field calculation software. However, the computation time quickly becomes an issue due to the large number of values to calculate and simulations to perform. This article introduces an innovative experimental approach to identify a coupled-circuit model and develop a digital twin of a wound-rotor induction machine. This method relies solely on simple electrical measurements and tests conducted at extremely low rotation speeds (1 rpm) to obtain inductance variations as a function of the rotor position. By employing this technique, the need for analytical models or finite-element field calculation simulations, which typically require precise knowledge of the machine’s geometry and materials, is circumvented. The measurement processing employs optimization methods to extract the inductances as a function of the rotor position, which are then used as input data for the coupled-circuit model. The final parameters are specific to each machine and replicate all its manufacturing imperfections such as eccentricity and geometric or winding defects. This experimental identification method significantly enhances the model’s accuracy and reduces the usually required preliminary calculation time in a finite-element-based identification process.
Influence of the TABS Material, Design, and Operating Factors on an Office Room’s Thermal Performance
Authors: Bobula Mikołaj , Michalak Piotr , Wołoszyn Jerzy
Published: April 2024

Reducing energy consumption in residential and commercial buildings is an important research topic. Thermally activated building systems are a promising technology for significantly reducing energy consumption. The high thermal inertia, large surfaces, and radiative nature are advantages of these systems, but, on the other hand, this makes the system control and design complex. A transient simulation is also required to address the dynamic behavior of the system. The influence of 19 factors (material, design, and operating parameters) on the air temperature and mean radiant temperature inside the room as well as the required cooling equipment power were analyzed to better understand the system. The screening experiment was conducted using the random balance design method, and measurement data were used to validate the resistance–capacitance model. The analysis was performed using the Plackett–Burman design and a design with randomly selected points from a full factorial experiment. The results show that internal heat gains and the inlet water temperature have a significant influence on the system, and the influence of the screed’s properties is insignificant compared to other parameters. It should be borne in mind that the obtained results and conclusions are valid for the assumed range of factors’ variability.
Analysis of the Influence of Insulation Moisture during Long-Term Exploitation of Transformers on Their Reliability as Determined by Alternating Current Electrical Parameters
Authors: Zukowski Pawel , Kierczynski Konrad , Rogalski Przemyslaw , Bondariev Vitalii , Zenker Marek , Pajak Rafal , Szrot Marek , Molenda Pawel , Koltunowicz Tomasz N.
Published: April 2024

This paper presents the results of the study of the direct current (DC) and alternating current (AC) electrical properties of an electrical pressboard–bio-insulating oil–water composite in a wide range of water content and temperatures used in electric power transformers. These parameters allow the level of insulation reliability to be determined after many years of operation of power transformers. To analyse the experimental results, a model of the DC and AC conductivities of nanocomposites based on the quantum-mechanical phenomenon of electron tunnelling was used. It was found that in a low-frequency region, the conductivities of AC and DC and their activation energy are equal. The relaxation times of AC conductivity and permittivity are also equal. It was found that the dependence of the DC conductivity on the distance between water molecules is an exponential function. On the basis of the model of conductivity by electron tunnelling between potential wells, the average number of water molecules in a nanodroplet, located in a composite of electrical pressboard–bio-insulating oil–moisture was determined to be (126 ± 20). It was found that the measured dependencies of DC and AC conductivity, permeability and dielectric relaxation times are consistent with the results of computer simulations performed on the basis of the model. This study showed that the composite of pressboard impregnated with bio-oil spontaneously transforms through water absorption into a pressboard–bio-oil–water nanocomposite. These will serve as the basis for the application of actual conductivity and dielectric relaxation mechanisms to improve the accuracy of moisture estimation in the solid component of power transformer insulation carried out on the basis of measurements of DC and AC properties. This will improve the operational safety of the transformers, minimise the occurrence of transformer failure and the associated environmental pollution.