bluuebunny/crossref_metadata_2025 huggingface dataset:
Novel Acrylamide/2-Acrylamide-2-3 Methylpropanesulfonic Acid/Styrene/Maleic Anhydride Polymer-Based CaCO3 Nanoparticles to Improve the Filtration of Water-Based Drilling Fluids at High Temperature
Authors: Tang Zhichuan , Qiu Zhengsong , Zhong Hanyi , Mao Hui , Shan Kai , Kang Yujie
Published: May 2022

Filtration loss control under high-temperature conditions is a worldwide issue among water-based drilling fluids (WBDFs). A core–shell high-temperature filter reducer (PAASM-CaCO3) that combines organic macromolecules with inorganic nanomaterials was developed by combining acrylamide (AM), 2-acrylamide-2-methylpropane sulfonic acid (AMPS), styrene (St), and maleic anhydride (MA) as monomers and nano-calcium carbonate (NCC). The molecular structure of PAASM-CaCO3 was characterized. The average molecular weight of the organic part was 6.98 × 105 and the thermal decomposition temperature was about 300 °C. PAASM-CaCO3 had a better high-temperature resistance. The rheological properties and filtration performance of drilling fluids treated with PAASM-CaCO3 were stable before and after aging at 200 °C/16 h, and the effect of filtration control was better than that of commonly used filter reducers. PAASM-CaCO3 improved colloidal stability and mud cake quality at high temperatures.
Novel Gene Rearrangements in the Mitochondrial Genomes of Cynipoid Wasps (Hymenoptera: Cynipoidea)
Authors: Shu Xiaohan , Li Zekai , Yuan Ruizhong , Tang Pu , Chen Xuexin
Published: May 2022

Cynipoidea is a medium-sized superfamily of Hymenoptera with diverse lifestyles. In this study, 16 mitochondrial genomes were newly sequenced, 11 of which were the first obtained mitochondrial genomes in the family Liopteridae and four subfamilies (Anacharitinae, Aspicerinae, Figitinae, and Parnipinae) of Figitidae. All of the newly sequenced mitogenomes have unique rearrangement types within Cynipoidea, whereas some gene patterns are conserved in several groups. nad5-nad4-nad4L-nad6-cytb was remotely inverted and two rRNA genes were translocated to nad3 downstream in Ibaliidae and three subfamilies (Anacharitinae, Eucoilinae, and Parnipinae within Figitidae); two rRNA genes in Aspicerinae, Figitinae, and Liopteridae were remotely inverted to the cytb-nad1 junction; rrnL-rrnS was translocated to the cytb-nad1 junction in Cynipidae. Phylogenetic inference suggested that Figitidae was a polyphyletic group, while the Ibaliidae nested deep within Cynipoidea and was a sister-group to the Figitidae. These results will improve our understanding of the gene rearrangement of the mitogenomes and the phylogenetic relationships in the Cynipoidea.
Expression Pattern of nos1 in the Developing Nervous System of Ray-Finned Fish
Authors: Annona Giovanni , Ferran José Luis , De Luca Pasquale , Conte Ivan , Postlethwait John H. , D’Aniello Salvatore
Published: May 2022

Fish have colonized nearly all aquatic niches, making them an invaluable resource to understand vertebrate adaptation and gene family evolution, including the evolution of complex neural networks and modulatory neurotransmitter pathways. Among ancient regulatory molecules, the gaseous messenger nitric oxide (NO) is involved in a wide range of biological processes. Because of its short half-life, the modulatory capability of NO is strictly related to the local activity of nitric oxide synthases (Nos), enzymes that synthesize NO from L-arginine, making the localization of Nos mRNAs a reliable indirect proxy for the location of NO action domains, targets, and effectors. Within the diversified actinopterygian nos paralogs, nos1 (alias nnos) is ubiquitously present as a single copy gene across the gnathostome lineage, making it an ideal candidate for comparative studies. To investigate variations in the NO system across ray-finned fish phylogeny, we compared nos1 expression patterns during the development of two well-established experimental teleosts (zebrafish and medaka) with an early branching holostean (spotted gar), an important evolutionary bridge between teleosts and tetrapods. Data reported here highlight both conserved expression domains and species-specific nos1 territories, confirming the ancestry of this signaling system and expanding the number of biological processes implicated in NO activities.
Annotation and Analysis of 3902 Odorant Receptor Protein Sequences from 21 Insect Species Provide Insights into the Evolution of Odorant Receptor Gene Families in Solitary and Social Insects
Authors: Mier Pablo , Fontaine Jean-Fred , Stoldt Marah , Libbrecht Romain , Martelli Carlotta , Foitzik Susanne , Andrade-Navarro Miguel A.
Published: May 2022

The gene family of insect olfactory receptors (ORs) has expanded greatly over the course of evolution. ORs enable insects to detect volatile chemicals and therefore play an important role in social interactions, enemy and prey recognition, and foraging. The sequences of several thousand ORs are known, but their specific function or their ligands have only been identified for very few of them. To advance the functional characterization of ORs, we have assembled, curated, and aligned the sequences of 3902 ORs from 21 insect species, which we provide as an annotated online resource. Using functionally characterized proteins from the fly Drosophila melanogaster, the mosquito Anopheles gambiae and the ant Harpegnathos saltator, we identified amino acid positions that best predict response to ligands. We examined the conservation of these predicted relevant residues in all OR subfamilies; the results showed that the subfamilies that expanded strongly in social insects had a high degree of conservation in their binding sites. This suggests that the ORs of social insect families are typically finely tuned and exhibit sensitivity to very similar odorants. Our novel approach provides a powerful tool to exploit functional information from a limited number of genes to study the functional evolution of large gene families.
Problematic Use of the Internet Mediates the Association between Reduced Mentalization and Suicidal Ideation: A Cross-Sectional Study in Young Adults
Authors: Bersani Francesco Saverio , Accinni Tommaso , Carbone Giuseppe Alessio , Corazza Ornella , Panno Angelo , Prevete Elisabeth , Bernabei Laura , Massullo Chiara , Burkauskas Julius , Tarsitani Lorenzo , Pasquini Massimo , Biondi Massimo , Farina Benedetto , Imperatori Claudio
Published: May 2022

Suicide is a major public health problem, and it is urgent to investigate its underlying clinical and psychological concomitants. It has been suggested that low mentalization skills and problematic use of the internet (PUI) are factors that can play a role in suicidal behaviors. It is possible that poor mentalization skills contribute to leading to forms of PUI, which, in turn, can represent triggers for suicidal ideation (SI). We tested this hypothesis through a quantitative and cross-sectional study on a sample (n = 623) of young adults (age range: 18–34). Self-report measures investigating symptoms related to Social Media Addiction (SMA), Internet Gaming Disorder (IGD), mentalization capacity, and SI were used. A single mediation analysis with two mediators was carried out to evaluate the direct and indirect effects of mentalization on SI through the mediating role of SMA- and IGD-related symptoms, controlling for potential confounding factors (e.g., socio-demographic and addiction-related variables). The four explored variables were significantly associated with each other (all p < 0.001) across all subjects; the mediational model showed that the total effect of mentalization on SI was significant (B = −0.821, SE = 0.092 (95% CI: −1.001; −0.641)) and that both SMA- (B = −0.073, SE = 0.034 (95% CI: −0.145; −0.008)) and IGD-related symptoms (B = 0.046, SE = 0.027 (95% CI: −0.107; −0.001)) were significant mediators of such association. Our findings support the possibility that PUI severity plays a relevant role in mediating the association between low mentalization skills and levels of SI.
Relationship between Sensitivity Tendency and Psychological Stress Reactivity in Healthy Students
Authors: Ishibashi Syunsaku , Murata Jun , Tokunaga Akiko , Imamura Akira , Kawano Kojiro , Iwanaga Ryoichiro , Tanaka Goro
Published: May 2022

This study examined the relationship between sensory processing sensitivity and psychological stress reactivity in 69 healthy Japanese university students. The Japanese version of the Highly Sensitive Person Scale and the Japanese version of the Adolescent/Adult Sensory Profile were used for subjective assessment. The Galvanic skin response was measured as an objective measure of stress responses while the participants were completing the Stroop task. The Wilcoxon signed-rank test, the Spearman rank correlation coefficient, and the Mann–Whitney U test were conducted for data analysis. The results demonstrated that there was no significant correlation between the Japanese version of the Highly Sensitive Person Scale and Galvanic skin response. However, there was a marginal trend toward significance between low registration in the Japanese version of the Adolescent/Adult Sensory Profile and Galvanic skin response (rs = 0.231, p < 0.10; rs = 0.219, p < 0.10), suggesting that self-rated sensitivity was not necessarily associated with objective measures. These results indicate that sensory processing sensitivity analyses require the consideration of the traits and characteristics of the participants and multifaceted evaluations using a sensitivity assessment scale other than the Japanese version of the Highly Sensitive Person Scale.
Diaphragmatic Mobility and Chest Expansion in Patients with Scapulocostal Syndrome: A Cross-Sectional Study
Authors: Srijessadarak Thanaporn , Arayawichanon Preeda , Kanpittaya Jaturat , Boonprakob Yodchai
Published: May 2022

Scapulocostal syndrome (SCS) is a subset of myofascial pain syndrome affecting the posterior shoulder and upper back area. Some of the affected muscles are attached to the rib cage, which may affect diaphragmatic mobility and chest expansion. The purpose of this study was to investigate the characteristics of diaphragmatic mobility and chest expansion in patients with SCS. Twenty-nine patients with SCS and twenty-nine healthy participants of a similar age, gender, weight, and height were included in the study. All participants were evaluated for diaphragmatic mobility (DM) by real-time ultrasound (RTUS) and for chest expansion (CE) using a cloth tape measure. An independent t-test was used to compare the outcome variables between groups. The DM value in the SCS group was 46.24 ± 7.26 mm, whereas in the healthy group it was 54.18 ± 9.74 mm. The DM value was lower in the SCS group compared to in healthy participants (p < 0.05). Chest expansion at the axilla, the fourth intercostal space (4th ICS), and the xiphoid level in the SCS group was 7.26 ± 1.13, 6.83 ± 0.94, and 6.86 ± 1.25, respectively, while chest expansion at the axilla, 4th ICS, and xiphoid level in the healthy group was 7.92 ± 1.39, 7.54 ± 1.43, and 8.13 ± 1.32, respectively. Chest expansion at the 4th ICS and the xiphoid level in the SCS group was significantly lower than in the healthy group (p < 0.05). Patients with SCS presented a decrease in diaphragmatic mobility and chest expansion. Therefore, SCS treatment programs ought to add breathing exercises to improve lung expansion.
Towards a Negative History of Science: The Unknown, Errors, Ignorance, and the “Pseudosciences”
Authors: Rathjen Lukas , Stähelin Jonas
Published: May 2022

This article outlines elements of a negative history of science. For historians wishing to get a fuller picture of scientific practice both internally and externally, there is a lot to be gained by considering the dialectical constitution of scientific knowledge. To fully comprehend this relationality, historians should, therefore, trace the negative relations science maintains. Through oppositions, such as known/unknown; success/error; consideration/ignorance; and inclusion/exclusion, scientific knowledge emerges and disappears, and the social position of scientific practice is both established and contested. To exemplify our argument, we present four areas: the unknown, errors, ignorance, and the “pseudosciences”. Taken together, this approach allows us to understand how science constitutes itself epistemically and socially across different locations and historical periods.
Comprehensive Analysis of N6-Methyladenosine Regulatory Genes from Citrus grandis and Expression Profilings in the Fruits of “Huajuhong” (C. grandis “Tomentosa”) during Various Development Stages
Authors: Tian Yuzhen , Zeng Jiwu , Fan Ruiyi
Published: May 2022

Citrus grandis “Tomentosa” (“Huajuhong”) is a famous traditional Chinese medicine. The aim of the present study is to provide a comprehensive characterization of the m6A regulatory genes from C. grandis, and examine their expression patterns in fruits of C. grandis “Tomentosa” during various developmental stages. A total of 26 N6-methyladenosine (m6A) regulatory proteins were identified from the genome of C. grandis, which were distributed across nine chromosomes in C. grandis. Phylogenetic relationships revealed that all m6A regulatory genes were divided into groups of m6A writers, erasers, and readers. The m6A writer groups included CgMTA, CgMTB, and CgMTC three MTs (methyltransferases), one CgVIR (virilizer), one CgHAKAI (E3 ubiquitin ligase HAKAI), and one CgFIP37 (FKBP interacting protein 37). Moreover, 10 CgALKBH (α-ketoglutarate-dependent dioxygenase homolog) members (numbered from CgALKBH1 to CgALKBH10) and 10 CgECT (C-terminal region) members (numbered from CgECT1 to CgECT10) in C. grandis were identified as m6A erasers and readers, respectively. The domain structures and motif architectures among the groups of m6A writers, erasers, and readers were diverse. Cis-acting elements in the promoters of the 26 m6A regulatory genes predicted that the abscisic acid-responsive (ABA) element (ABRE) was present on the promoters of 19 genes. In addition, the expression profiles of all m6A regulatory genes were examined in the fruits of two varieties of C. grandis “Tomentosa” during different growth stages to give basic hints for further investigation of the function of the N6-methyladenosine regulatory genes in C. grandis “Tomentosa”.
Neural Network-Based Modeling of Water Quality in Jodhpur, India
Authors: Sinha Krishna Kumar , Gupta Mukesh Kumar , Banerjee Malay Kumar , Meraj Gowhar , Singh Suraj Kumar , Kanga Shruti , Farooq Majid , Kumar Pankaj , Sahu Netrananda
Published: May 2022

In this paper, the quality of a source of drinking water is assessed by measuring eight water quality (WQ) parameters using 710 samples collected from a water-stressed region of India, Jodhpur Rajasthan. The entire sample was divided into ten groups representing different geographic locations. Using American Public Health Association (APHA) specified methodology, eight WQ parameters, viz., pH, total dissolved solids (TDS), total alkalinity (TA), total hardness (TH), calcium hardness (Ca-H), residual chlorine, nitrate (as NO3−), and chloride (Cl−), were selected for describing the water quality for potability use. The quality of each parameter is examined as a function of the zone. Taking the average parametric values of different zones, a unique number was used to describe the overall quality of water. It was found that the average value of each parameter varies significantly with zones. Further, we used neural network (NN) modeling to map the nonlinear relationship between the above eight parametric inputs and the water quality index as the output. It can be observed that the NN designed in the present work acquired sufficient learning and can be satisfactorily used to predict the relational pattern between the input and the output. It can further be observed that the water quality index (WQI) from this work is highly efficient for a successful assessment of water quality in the study area. The major challenge to uniquely describing the drinking water quality lies in understanding the cumulative effect of various parameters affecting the quality of water; the quantified figure is subjected to debate, and this paper addresses the difficulty through a novel approach. The framework presented in this work can be automated with appropriate equipment and shall help government agencies understand changing water quality for better management.
Associations among Perceived Sexual Stigma from Family and Peers, Internalized Homonegativity, Loneliness, Depression, and Anxiety among Gay and Bisexual Men in Taiwan
Authors: Lin Huang-Chi , Chang Chih-Cheng , Chang Yu-Ping , Chen Yi-Lung , Yen Cheng-Fang
Published: May 2022

This study aimed to examine the moderating factors of the association between perceived sexual stigma from family and peers and internalized homonegativity, as well as to compare the effects of perceived sexual stigma from family and peers and internalized homonegativity on loneliness, depression, and anxiety in gay and bisexual men. In total, 400 gay and bisexual men participated in this study. The experiences of perceived sexual stigma from family and peers on the Homosexuality subscale of the HIV and Homosexuality Related Stigma Scale, internalized homonegativity on the Measure of Internalized Sexual Stigma for Lesbians and Gay Men, loneliness on the UCLA Loneliness Scale (Version 3), depression on the Center for Epidemiological Studies-Depression Scale, and anxiety on the State subscale of the State-Trait Anxiety Inventory were collected. The results indicated that perceived sexual stigma from family and peers was significantly associated with internalized homonegativity in both gay and bisexual men, and that sexual orientation moderated the association. Moreover, the association between internalized homonegativity and loneliness was significantly greater than that between perceived sexual stigma from family and peers and loneliness, although no significant differences were observed in their associations with depression and anxiety. Intervention programs that promote changes in the attitudes toward gay and bisexual men among the general population are needed to help prevent the development of internalized homonegativity and further loneliness, depression and anxiety.
Drivers of Household Recycling Behavior in the City of Johannesburg, South Africa
Authors: Schoeman Dorothea Christina , Rampedi Isaac Tebogo
Published: May 2022

This paper has assessed the relationship between recycling behavior and socio-demographic variables for households in Johannesburg, South Africa. The research also identified the underlying driving factors that motivate recyclers to separate their household waste for recycling. These objectives were addressed by means of a quantitative survey research design as well as descriptive and inferential statistical methods. Based on the results, the statements that represented attitudes, subjective norms, perceived control, moral norms, situational factors, outcomes, and consequences of recycling were highly agreed to by respondents. Three factors influencing household recycling behavior were identified, namely, recycling benefits, perceived control, and situational variables. The recycling benefits construct explained 45.6% of the variance, followed by the perceived control construct (12%) and the situational variables construct explained 11% of the variance. However, the results of the EFA and multiple regression identified the situational variable as the greatest single driver of household participation in recycling. It is therefore important to overcome situational barriers that the residents of Johannesburg are currently facing if the current household waste separating program is to become successful. This would require simplifying the process of household recycling by providing appropriate knowledge, infrastructure, and the space for waste sorting and its collection.
Translation and Cross-Cultural Adaptation of the Cancer Health Literacy Test for Portuguese Cancer Patients: A Pre-Test
Authors: Barros Ana , Santos Helena , Moreira Luís , Santos-Silva Filipe
Published: May 2022

Assuming the multidimensionality of health literacy, new complex and comprehensive approaches are more adequate to specific disease contexts, such as cancer. Assessing cancer literacy levels is a priority, since it entails potential serious implications for disease outcomes and patient’s quality of life. This article reports on the translation and cultural adaptation of the Cancer Health Literacy Test to measure cancer literacy in Portuguese cancer patients. A multidisciplinary team of experts ensured the translation and cultural adaptation of the CHLT-30. A pre-test was conducted in two stages to evaluate the Portuguese version (CHLT-30 PT) in a sample of cancer patients (n = 71). Descriptive statistics were used to characterize the sample. Reliability (test–retest and internal consistency) and construct validity of CHLT-30 PT were assessed. The results obtained show a good internal consistency of the tool, respectively (Cronbach’s alpha = 0.86 in the test and 0.80 in the retest). Patients’ raw score mean in both test (23.96) and retest (25.97) and the distribution of scores categories are not statistically different. A suggestive association between higher education level and better total score was found compared to the results reported in CHLT-30-DKspa. The results obtained in the pre-test are favorable, and the instrument is now suitable for the next steps of the validation process. A Portuguese version of this tool will allow outlining patients’ cancer literacy along the cancer care continuum, enabling the identification and implementation of adequate socio-educational strategies with highly positive impacts on health outcomes.
Physical Activity and Food Environments in and around Schools: A Case Study in Regional North-West Tasmania
Authors: Jayasinghe Sisitha , Flies Emily J. , Soward Robert , Kendal Dave , Kilpatrick Michelle , Cleland Verity , Roberts Rebecca , Norzahari Fadhillah , Davern Melanie , Holloway Timothy P. , Murray Sandra , Patterson Kira A. E. , Ahuja Kiran D. K. , Hughes Roger , Byrne Nuala M. , Hills Andrew P.
Published: May 2022

A better understanding of the physical activity (PA) infrastructure in schools, the walkability of neighborhoods close to schools, and the food environments around schools, particularly in rural, socioeconomically challenged areas such as the North-West (NW) of Tasmania, could be important in the wider effort to improve the health of school-age children. Accordingly, this research aimed to assess PA resources, walkability, and food environments in and around schools in three socioeconomically disadvantaged, regional/rural Local Government Areas (LGAs) of Tasmania, Australia. A census of schools (including assessment of the PA infrastructure quality within school grounds), a walkability assessment, and a census of food outlets surrounding schools (through geospatial mapping) were executed. Most of the schools in the study region had access to an oval, basketball/volleyball/netball court, and free-standing exercise equipment. In all instances (i.e., regardless of school type), the quality of the available infrastructure was substantially higher than the number of incivilities observed. Most schools also had good (i.e., within the first four deciles) walkability. Numerous food outlets were within the walking zones of all schools in the study region, with an abundance of food outlets that predominantly sold processed unhealthy food.
Effect of Attentional Focus on Sprint Performance: A Meta-Analysis
Authors: Li Danyang , Zhang Liwei , Yue Xin , Memmert Daniel , Zhang Yeqin
Published: May 2022

Sprinting is often seen in a variety of sports. Focusing one’s attention externally before sprinting has been demonstrated to boost sprint performance. The present study aimed to systematically review previous findings on the impact of external focus (EF), in comparison to internal focus (IF), on sprint performance. A literature search was conducted in five electronic databases (APA PsycINFO, PubMed, Scopus, SPORTDiscus, and Web of Science). A random-effects model was used to pool Hedge’s g with 95% confidence intervals (CIs). The meta-analysis included six studies with a total of 10 effect sizes and 166 participants. In general, the EF condition outperformed the IF condition in sprint performance (g = 0.279, 95% CI [0.088, 0.470], p = 0.004). The subgroup analysis, which should be viewed with caution, suggested that the benefits associated with the EF strategy were significant in low-skill sprinters (g = 0.337, 95% CI [0.032, 0.642], p = 0.030) but not significant in high-skill sprinters (g = 0.246, 95% CI [−0.042, 0.533], p = 0.094), although no significant difference was seen between these subgroups (p = 0.670). The reported gain in sprint performance due to attentional focus has practical implications for coaches and athletes, as making tiny adjustments in verbal instructions can lead to significant behavioral effects of great importance in competitive sports.
On the Deterministic-Shift Extended CIR Model in a Negative Interest Rate Framework
Authors: Di Francesco Marco , Kamm Kevin
Published: May 2022

In this paper, we propose a new exogenous model to address the problem of negative interest rates that preserves the analytical tractability of the original Cox–Ingersoll–Ross (CIR) model with a perfect fit to the observed term-structure. We use the difference between two independent CIR processes and apply the deterministic-shift extension technique. To allow for a fast calibration to the market swaption surface, we apply the Gram–Charlier expansion to calculate the swaption prices in our model. We run several numerical tests to demonstrate the strengths of this model by using Monte-Carlo techniques. In particular, the model produces close Bermudan swaption prices compared to Bloomberg’s Hull–White one-factor model. Moreover, it finds constant maturity swap (CMS) rates very close to Bloomberg’s CMS rates.
The Use of Closed Incision Negative Pressure Therapy on the Medial Thigh Donor Site in Transverse Musculocutaneous Gracilis Flap Breast Reconstruction
Authors: Siegwart Laura Cosima , Tapking Christian , Diehm Yannick Fabian , Haug Valentin Felix , Bigdeli Amir Khosrow , Kneser Ulrich , Kotsougiani-Fischer Dimitra
Published: May 2022

The objective of this study was to examine the impact of closed incision negative pressure therapy (CINPT) on donor site complications and patient perceptions in transverse musculocutaneous gracilis (TMG) flap breast reconstruction. Our institution conducted a retrospective cohort study, including all patients with TMG flap breast reconstruction from 1 January 2010 to 31 December 2021. Patients were grouped according to conventional wound management or CINPT. Outcomes were surgical site complications, fluid drainage, time to drain removal, and in-hospital stay length. A patient survey was created. A total of 56 patients with 83 TMG flaps were included (control group: 35 patients with 53 TMG flaps; CINPT group: 21 patients with 30 TMG flaps). Patient characteristics were similar in both groups. The flap width was significantly larger in the CINPT group (8.0 cm vs. 7.0 cm, p = 0.013). Surgical site complications were reduced in the CINPT group without statistical difference (30.0% vs. 50.9%, p = 0.064). Fluid drainage and time to drain removal were similar in both groups. The average in-hospital stay was significantly shortened in the CINPT group (10.0 days vs. 13.0 days, p = 0.030). The survey excluded pain, skin irritations, and discomfort during sleep and movement in the CINPT group and showed that the patients felt well protected. This study fails to provide compelling evidence for CINPT to enhance incision healing on the donor site in TMG flap breast reconstruction. There was a trend toward reduced surgical site complications on the donor thigh and the in-hospital stay was shortened. Prophylactic CINPT increases patient comfort and provides a feeling of additional wound protection.
Breast and Thyroid Surgery in 2021 and Beyond
Authors: Fama’ Fausto
Published: May 2022

Several studies in the literature report the association between breast and thyroid pathologies; however, the underlying causes (genetic, environmental, hormonal or immunological) have not yet been well explicated [...]
Image Augmentation Techniques for Mammogram Analysis
Authors: Oza Parita , Sharma Paawan , Patel Samir , Adedoyin Festus , Bruno Alessandro
Published: May 2022

Research in the medical imaging field using deep learning approaches has become progressively contingent. Scientific findings reveal that supervised deep learning methods’ performance heavily depends on training set size, which expert radiologists must manually annotate. The latter is quite a tiring and time-consuming task. Therefore, most of the freely accessible biomedical image datasets are small-sized. Furthermore, it is challenging to have big-sized medical image datasets due to privacy and legal issues. Consequently, not a small number of supervised deep learning models are prone to overfitting and cannot produce generalized output. One of the most popular methods to mitigate the issue above goes under the name of data augmentation. This technique helps increase training set size by utilizing various transformations and has been publicized to improve the model performance when tested on new data. This article surveyed different data augmentation techniques employed on mammogram images. The article aims to provide insights into basic and deep learning-based augmentation techniques.
Extreme Waves
Authors: Ponce de León Sonia , Young Ian R. , Waseda Takuji , Osborne Alfred R.
Published: May 2022

The occurrence of exceptionally large waves in regions of high maritime traffic has severe consequences, ranging from complicating navigation routes to the loss of ships and human lives [...]
The Force Exerted by Surface Wave on Cylinder and Its Parameterization: Morison Equation Revisited
Authors: Zan Xiaoxiao , Lin Zhenhua , Gou Ying
Published: May 2022

The Morison equation is widely used to estimate the loads by surface waves on cylinders. The formulation and coefficients determination method of the original work by Morison et al. are revisited, it is found that there exist some issues yet to be explained, e.g., the larger uncertainties in drag coefficient and the underestimated maximum moments. Numerical simulation with a similar configuration is used to reproduce these issues and the results help discover the reason and mechanism for these phenomena. The analysis shows that the larger uncertainties in drag coefficient are caused by the introduction of linear wave theory, which is used to derive the velocity and acceleration at cylinder location as direct measurements are not available. The results also show that the underestimation of maximum moments is induced by the wave run-up process, which is neglected in the Morison equation. The scale of wave run-up is approximately the length of cylinder diameter. The results indicate although most recent studies are focusing on the high-frequency loads on cylinders by nonlinear waves, there still exist some issues to be resolved in the linear wave regime. Further studies are required to parameterize the additional loads by wave run-up to strengthen the robustness of the Morison equation.
Energetic and Ecological Effects of the Slow Steaming Application and Gasification of Container Ships
Authors: Gospić Ivan , Martić Ivana , Degiuli Nastia , Farkas Andrea
Published: May 2022

One of the short-term operational measures for fuel savings and reducing CO2 emissions from ships at sea is sailing at reduced speed, i.e., slow steaming, while the gasification of the ship represents an important mid-term technical measure. In this study, the energetic and ecological benefits of slow steaming and gasification are studied for a container ship sailing between Shanghai and Hamburg. Resistance and propulsion characteristics in calm water are calculated using computational fluid dynamics based on the viscous flow theory for a full-scale ship, while the added resistance in waves is calculated by applying potential flow theory. The propeller operating point is determined for the design and slow steaming speeds at sea states with the highest probability of occurrence through the investigated sailing route. Thereafter, the fuel consumption and CO2 emissions are calculated for a selected dual fuel engine in fuel oil- and gas-supplying modes complying with IMO Tier II and Tier III requirements. The results demonstrate a significant reduction in fuel consumption and CO2 emissions for various slow steaming speeds compared to the design speed at different sea states, and for the gasification of a container ship. For realistic weather conditions through the investigated route, the potential reduction in CO2 emissions per year could be up to 11.66 kt/year for fuel oil mode and 8.53 kt/year for gas-operating mode. CO2 emission reduction per year due to gasification under realistic weather conditions could be up to 22 kt/year.
Ustilago maydis Metabolic Characterization and Growth Quantification with a Genome-Scale Metabolic Model
Authors: Liebal Ulf W. , Ullmann Lena , Lieven Christian , Kohl Philipp , Wibberg Daniel , Zambanini Thiemo , Blank Lars M.
Published: May 2022

Ustilago maydis is an important plant pathogen that causes corn smut disease and serves as an effective biotechnological production host. The lack of a comprehensive metabolic overview hinders a full understanding of the organism’s environmental adaptation and a full use of its metabolic potential. Here, we report the first genome-scale metabolic model (GSMM) of Ustilago maydis (iUma22) for the simulation of metabolic activities. iUma22 was reconstructed from sequencing and annotation using PathwayTools, and the biomass equation was derived from literature values and from the codon composition. The final model contains over 25% annotated genes (6909) in the sequenced genome. Substrate utilization was corrected by BIOLOG phenotype arrays, and exponential batch cultivations were used to test growth predictions. The growth data revealed a decrease in glucose uptake rate with rising glucose concentration. A pangenome of four different U. maydis strains highlighted missing metabolic pathways in iUma22. The new model allows for studies of metabolic adaptations to different environmental niches as well as for biotechnological applications.
Secretome Profiling by Proteogenomic Analysis Shows Species-Specific, Temperature-Dependent, and Putative Virulence Proteins of Pythium insidiosum
Authors: Krajaejun Theerapong , Rujirawat Thidarat , Lohnoo Tassanee , Yingyong Wanta , Sae-Chew Pattarana , Reamtong Onrapak , Kittichotirat Weerayuth , Patumcharoenpol Preecha
Published: May 2022

In contrast to most pathogenic oomycetes, which infect plants, Pythium insidiosum infects both humans and animals, causing a difficult-to-treat condition called pythiosis. Most patients undergo surgical removal of an affected organ, and advanced cases could be fetal. As a successful human/animal pathogen, P. insidiosum must tolerate body temperature and develop some strategies to survive and cause pathology within hosts. One of the general pathogen strategies is virulence factor secretion. Here, we used proteogenomic analysis to profile and validate the secretome of P. insidiosum, in which its genome contains 14,962 predicted proteins. Shotgun LC–MS/MS analysis of P. insidiosum proteins prepared from liquid cultures incubated at 25 and 37 °C mapped 2980 genome-predicted proteins, 9.4% of which had a predicted signal peptide. P. insidiosum might employ an alternative secretory pathway, as 90.6% of the validated secretory/extracellular proteins lacked the signal peptide. A comparison of 20 oomycete genomes showed 69 P. insidiosum–specific secretory/extracellular proteins, and these may be responsible for the host-specific infection. The differential expression analysis revealed 14 markedly upregulated proteins (particularly cyclophilin and elicitin) at body temperature which could contribute to pathogen fitness and thermotolerance. Our search through a microbial virulence database matched 518 secretory/extracellular proteins, such as urease and chaperones (including heat shock proteins), that might play roles in P. insidiosum virulence. In conclusion, the identification of the secretome promoted a better understanding of P. insidiosum biology and pathogenesis. Cyclophilin, elicitin, chaperone, and urease are top-listed secreted/extracellular proteins with putative pathogenicity properties. Such advances could lead to developing measures for the efficient detection and treatment of pythiosis.
Association of a Common NOS1AP Variant with Attenuation of QTc Prolongation in Men with Heroin Dependence Undergoing Methadone Treatment
Authors: Chang Kuan-Cheng , Chen Ke-Wei , Huang Chieh-Liang , Liao Wen-Ling , Wu Mei-Yao , Lin Yu-Kai , Shiao Yi-Tzone , Chung Wei-Hsin , Lin Yen-Nien , Lane Hsien-Yuan
Published: May 2022

Background: The effects of methadone-induced severe prolongation of the corrected QT interval (QTc) and sudden cardiac death appear unpredictable and sex-dependent. Genetic polymorphisms in the nitric oxide synthase 1 adaptor protein (NOS1AP) have been implicated in QTc prolongation in general populations. We investigated whether common NOS1AP variants interact with methadone in relation to QTc prolongation in patients with heroin dependence. Methods: We genotyped 17 NOS1AP variants spanning the entire gene in heroin-dependent patients who received a 12-lead electrocardiography (ECG) examination both at baseline and during maintenance methadone treatment in Cohort 1 and only during maintenance methadone treatment in Cohort 2. The QT interval was measured automatically by the Marquette 12SL program, and was corrected for heart rate using Bazett’s formula. Results: Cohort 1 consisted of 122 patients (age: 37.65 ± 8.05 years, 84% male, methadone dosage: 42.54 ± 22.17 mg/day), and Cohort 2 comprised of 319 patients (age: 36.9 ± 7.86 years, 82% male, methadone dosage: 26.08 ± 15.84 mg/day), with complete genotyping data for analyses. Before methadone, the QTc intervals increased with increasing age (r = 0.3541, p < 0.001); the age-adjusted QTc showed dose-dependent prolongation in men (r = 0.6320, p < 0.001), but abbreviation in women (r = −0.5348, p = 0.018) in Cohort 1. The pooled genotype-specific analysis of the two cohorts revealed that the QTc interval was significantly shorter in male carriers of the rs164148 AA variant than in male carriers of the reference GG genotype (GG: n = 262, QTc = 423 ± 1.4 ms; AA: n = 10, QTc = 404.1 ± 7 ms, p = 0.009), according to univariate analysis. The QTc remained shorter in male carriers of the rs164148 AA variant compared to GG genotype (423 ± 1.4 ms vs. 405.9 ± 6.9 ms, p = 0.016) in multivariate analysis after adjusting for age and methadone dosage. A cut-off QTc interval of <410 ms identifies 100% of AA carriers compared to none of GG carriers when receiving a daily methadone dosage of 30.6 ± 19.3 mg. There was no significant gene-drug interaction in contributing to the adjusted QTc (p = 0.2164) in male carriers of the rs164148 variants. Conclusions: Carriers of a common NOS1AP rs164148 AA genotype variant were associated with a shorter QTc interval in men receiving maintenance methadone treatment. This genetic polymorphism attenuates the QTc-prolonging effect by methadone, and thus may explain at least in part the unpredictable and heterogeneous risks for severe QTc prolongation and sudden cardiac death in patients on methadone.
Development of Risk Index and Risk Governance Index: Application in Indian Public Sector Undertakings
Authors: Maheshwari Suneel , Gupta Vasudha , Naik Deepak Raghava
Published: May 2022

The purpose of the paper is to develop a risk measure in the form of a risk index and a governance index as an indicator of the quality of governance structure. Using the Delphi technique, two indices are developed (risk index and corporate governance index (CGI)); subsequently, using the 10-year (2005–2015) data of top Indian Public Sector Undertakings (PSUs) and diff-GMM regression (to deal with endogeneity), indices have been validated. Though the data set may appear old, it has only been used to test the risk index and analyze the results. Empirical evidence on indices indicates that Indian PSUs have ‘moderate’ risk levels and ample scope for improvement in their governance structure. Further, a positive relation between governance index and returns and negative relation between risk index and returns lend credence to the indices developed in the study. Notably, the governance index appears to be a moderating variable in the relationship between risk and return. It is perhaps the first study to put forth a comprehensive measure of risk to measure risk levels of PSUs and prescribe a measure of the quality of governance structure. While constructing the CGI, certain non-compliances were observed, even in terms of mandatory requirements, such as the proportion of PSUs may take independent directors. The new datasets may further check for compliance and its effect on the results. Such infringements call for stringent penal provisions and better monitoring of PSUs. Further, if the normative frameworks are adhered to as per the study by the Securities and Exchange Board of India (SEBI) and Ministry of Corporate Affairs (MCA), more effective and efficient decisions with lower risks, and hassle-free management resulting in better return on assets and return on equity.
Human Amniotic Mesenchymal Stem Cells and Fibroblasts Accelerate Wound Repair of Cystic Fibrosis Epithelium
Authors: Beccia Elisa , Daniello Valeria , Laselva Onofrio , Leccese Giorgia , Mangiacotti Michele , Di Gioia Sante , La Bella Gianfranco , Guerra Lorenzo , Matteo Maria , Angiolillo Antonella , Conese Massimo
Published: May 2022

Cystic fibrosis (CF) airways are affected by a deranged repair of the damaged epithelium resulting in altered regeneration and differentiation. Previously, we showed that human amniotic mesenchymal stem cells (hAMSCs) corrected base defects of CF airway epithelial cells via connexin (CX)43-intercellular gap junction formation. In this scenario, it is unknown whether hAMSCs, or fibroblasts sharing some common characteristics with MSCs, can operate a faster repair of a damaged airway epithelium. A tip-based scratch assay was employed to study wound repair in monolayers of CFBE14o- cells (CFBE, homozygous for the F508del mutation). hAMSCs were either co-cultured with CFBE cells before the wound or added to the wounded monolayers. NIH-3T3 fibroblasts (CX43+) were added to wounded cells. HeLa cells (CX43-) were used as controls. γ-irradiation was optimized to block CFBE cell proliferation. A specific siRNA was employed to downregulate CX43 expression in CFBE cells. CFBE cells showed a delayed repair as compared with wt-CFTR cells (16HBE41o-). hAMSCs enhanced the wound repair rate of wounded CFBE cell monolayers, especially when added post wounding. hAMSCs and NIH-3T3 fibroblasts, but not HeLa cells, increased wound closure of irradiated CFBE monolayers. CX43 downregulation accelerated CFBE wound repair rate without affecting cell proliferation. We conclude that hAMSCs and fibroblasts enhance the repair of a wounded CF airway epithelium, likely through a CX43-mediated mechanism mainly involving cell migration.
Isolation, Characterization, Pharmacology and Biopolymer Applications of Licorice Polysaccharides: Review
Authors: Ain Noor Ul , Wu Shuye , Li Xiang , Li Duxin , Zhang Zhenqing
Published: May 2022

Licorice is known as “Gan-Cao” in traditional Chinese Medicine (TCM), belonging to the genus Glycyrrhiza (Family: Fabaceae/Leguminosae). It has a long medicinal history and wide applications in China. Polysaccharides of licorice (LPs) are one of the key bioactive components. As herbal polysaccharides attracted increasing interest in the past several decades, their extraction, isolation, structural characterization, pharmacological activities, and medicinal application have been explored extensively. It is worth heeding that the method of extraction and purification effects LPs, apart from specie and origin specificity. This review evaluates the method of extraction and purification and demonstrates its performance in gaining specific composition and its structure-activity relationship, which might lead the readers to a fresh horizon for developing advanced treatment strategies. It is recently reported that the conformation of LPs plays a vital role as biopolymers, such as selenized modification, microencapsulation, nanocomposite, liposome formulation, drug/hydrogel combinations, biosensor device, and synergistic effect with a vaccine. In addition, LPs showed a good thermodynamics profile, as these properties enable them to interact with additional supramolecular interaction by chemical modifications or copolymerization. Functional polymers that are responsive to various external stimuli, such as physical, chemical, and biological signals, are a promising study topic. Thus, LPs are emerging as a new biomaterial that can enhance intended formulation along exerting its inherent medicinal effects. It is hoped that this review will provide a basis for the utilization and further developments of licorice polysaccharides in the vast medium.
Conjugate Heat Transfer Simulation of Overall Cooling Performance for Cratered Film Cooling Holes
Authors: Zhang Chao , Wang Wenzhuang , Wang Zhan , Tong Zhiting
Published: May 2022

Film cooling is widely applied as an effective way to maintain the turbine blade temperature at an acceptable level. The present paper investigates the overall cooling effectiveness and flow structure by performing conjugate heat transfer simulations for the flat-plate baseline cylindrical and three cratered film-cooling holes. The flow and heat transfer in the fluid domain is obtained using the Shear Stress Transport turbulence model, and the solid conductivity is considered by solving the Laplace equation. Four blowing ratios ranging from 0.5 to 2.0 are studied. The numerical results show that the concentric elliptic cratered hole yields a slightly higher overall cooling effectiveness than the baseline cylindrical hole, but the two contoured cratered holes exhibit great improvements due to the generation of the anti-kidney-shaped vortex pair. The area-averaged overall cooling effectiveness has improved by 5.58–65.30% for the contoured cratered hole. The variation of Biot number results in small change in the area-averaged overall cooling effectiveness. However, the area-averaged overall cooling effectiveness uniformity coefficient depends on the Biot number.
A Mathematical Modeling and Analysis Method for the Kinematics of a Maglev Train
Authors: Liu Mingxin , Li Jie , Jin Yuxin , Leng Peng , Zhou Danfeng
Published: May 2022

In recent years, more and more countries are paying attention to maglev transportation because of its outstanding advantages. However, because the structure of a maglev train is more complex than a wheel-rail train, there is no systematic theory method for kinematic modeling of a maglev vehicle. Based on the screw theory and the product of exponentials (PoE) formula in the field of open-chain robotics, this paper presents a method of kinematic mathematical modeling and analysis for a maglev train and track. By analyzing the characteristics of the closed chain running mechanism of a maglev train, the kinematic mathematical model of a maglev train and track is established, and the motion state of each moving part of the vehicle on a typical track is calculated and analyzed. At last, compared with the results of experiment, the correctness of modeling method proposed in this paper is verified.