bluuebunny/crossref_metadata_2025 huggingface dataset:
Current and Future Roles of Glycoprotein IIb–IIIa Inhibitors in Primary Angioplasty for ST-Segment Elevation Myocardial Infarction
Authors: De Luca Giuseppe , Verburg Ashley , Hof Arnoud van’t , ten Berg Jurrien , Kereiakes Dean J. , Coller Barry S. , Gibson Charles Michael
Published: September 2024

Acute myocardial infarction still represents the major cause of mortality in high-income countries. Therefore, considerable efforts have been focused on the treatment of myocardial infarctions in the acute and long-term phase, with special attention being paid to reperfusion strategies and adjunctive antithrombotic therapies. In fact, despite the successful mechanical recanalization of the epicardial conduit, a substantial percentage of patients still experience poor myocardial reperfusion or acute/subacute in-stent thrombosis. Due the delayed onset of action of currently available oral antiplatelet therapies, glycoprotein (GP) IIb–IIIa inhibitors could be expected to improve clinical outcomes, especially when administrated in the early phase of the infarction, due to the larger platelet composition of fresh thrombi, the dynamic nature of early thrombi, and the larger amount of viable myocardium existing in the early, as compared to a delayed, phase. Considerable evidence has accumulated regarding the benefits from GP IIb–IIIa inhibitors on mortality, especially among high-risk patients and when administered as an upstream strategy. Therefore, based on currently available data, GP IIb–IIIa inhibitors can be considered when the drug can be administered within the first 3 h of symptom onset and among high-risk patients (e.g., those with advanced Killip class or an anterior myocardial infarction). Even though it is not universally accepted, in our opinion, this strategy should be implemented in a pre-hospital setting (in an ambulance) or as soon as possible when arriving at the hospital (at the Emergency Room or Coronary Care Unit, irrespective of whether they are in spoke or hub hospitals). A new, second-generation GP IIb–IIIa inhibitor (zalunfiban) appears to be highly suitable as a pre-hospital pharmacological facilitation strategy at the time of first medical contact due to its favourable features, including its simple subcutaneous administration, rapid onset of action (15 min), and limited time of action (with a half-life of ~1 h), which is likely to minimize the risk of bleeding. The ongoing CELEBRATE trial, including 2499 STEMI patients, may potentially provide compelling data to support the upstream treatment of STEMI patients undergoing mechanical reperfusion. In fact, although the current therapeutic target of increased rates of timely reperfusion has been achieved, the future goal in myocardial infarction treatment should be to achieve the most rapid reperfusion prior to primary percutaneous coronary intervention, thus further minimizing myocardial damage, or, in some cases, even preventing it completely, and improving survival.
Finite Element Analysis of the Bearing Component of Total Ankle Replacement Implants during the Stance Phase of the Gait Cycle
Authors: Jain Timothy S. , Noori Mohammad , Rencis Joseph J. , Anderson Amanda , Noori Naudereh , Hazelwood Scott
Published: September 2024

Total ankle arthroplasty (TAA) is a motion-preserving treatment for end-stage ankle arthritis. An effective tool for analyzing these implants’ mechanical performance and longevity in silico is finite element analysis (FEA). An FEA in ABAQUS was used to statically analyze the mechanical behavior of the ultra-high-molecular-weight polyethylene (UHMWPE) bearing component at varying dorsiflexion/plantarflexion ankle angles and axial loading conditions during the stance phase of the gait cycle for a single cycle. The von Mises stress and contact pressure were examined on the articulating surface of the bearing component in two newly installed fixed-bearing TAA implants (Wright Medical INBONE II and Exactech Vantage). Six different FEA models of variable ankle compressive load levels and ankle angle positions, for the varying subphases of the stance phase of the gait cycle, were created. The components in these models were constrained to be conducive to the bone–implant interface, where implant loosening occurs. Our results showed that the von Mises stress and contact pressure distributions increased as the compressive load increased. The highest stress was noted at dorsiflexion angles > 15°, in areas where the UHMWPE liner was thinnest, at the edges of the talar and UHMWPE components, and during the terminal stance phase of the gait cycle. This static structural analysis highlighted these failure regions are susceptible to yielding and wear and indicated stress magnitudes that are in agreement (within 25%) with those in previous static structural TAA FEAs. The mechanical wear of the UHMWPE bearing component in TAA can lead to aseptic loosening and peri-implant cyst formation over time, requiring surgical revision. This study provides ankle replacement manufacturers and orthopedic surgeons with a better understanding of the stress response and contact pressure sustained by TAA implants, which is critical to optimizing implant longevity and improving patient care.
Optimization of Nanowell-Based Label-Free Impedance Biosensor Based on Different Nanowell Structures
Authors: Fardoost Ali , Raji Hassan , Javanmard Mehdi
Published: September 2024

Nanowell-based impedance-based label-free biosensors have demonstrated significant advantages in sensitivity, simplicity, and accuracy for detecting cancer biomarkers and macromolecules compared to conventional impedance-based biosensors. Although nanowell arrays have previously been employed for biomarker detection, a notable limitation exists in the photolithography step of their fabrication process, leading to a reduced efficiency rate. Historically, the diameter of these nanowells has been 2 μm. To address this issue, we propose alternative geometries for nanowells that feature larger surface areas while maintaining a similar circumference, thereby enhancing the fabrication efficiency of the biosensors. We investigated three geometries: tube, spiral, and quatrefoil. Impedance measurements of the samples were conducted at 10 min intervals using a lock-in amplifier. The study utilized interleukin-6 (IL-6) antibodies and antigens/proteins at a concentration of 100 nM as the target macromolecules. The results indicated that tube-shaped nanowells exhibited the highest sensitivity for detecting IL-6 protein, with an impedance change of 9.55%. In contrast, the spiral, quatrefoil, and circle geometries showed impedance changes of 0.91%, 0.95%, and 1.62%, respectively. Therefore, the tube-shaped nanowell structure presents a promising alternative to conventional nanowell arrays for future studies, potentially enhancing the efficiency and sensitivity of biosensor fabrication.
How Distressed Are Adolescent Students? A Mix-Method Study on High School Students in Northern Italy, Two Years after the Beginning of the COVID-19 Pandemic
Authors: Della Vedova Anna Maria , Covolo Loredana , Fiammenghi Carlotta , Marconi Silvia , Gelatti Umberto , Castellano Maurizio , Zanini Barbara
Published: September 2024

Adolescence is a central phase for the development of a person’s identity, involving complex multidimensional changes and increasing vulnerability to distress. This study aimed to investigate the psychological well-being of adolescent students in Brescia (Northern Italy), two years after the beginning of the COVID-19 pandemic. An online survey investigated the extent and the reasons reported by high school students (13–21 years) for their emotional distress (ED), also considering other factors such as physical activity, nutrition, sleep and smartphone overuse. The main reasons for ED were classified through a qualitative analysis of the free-text answers. A total of 1686 students agreed to participate, and 50% showed a presence of ED. According to a multiple logistic analysis, adolescents were more at risk of ED if they were female (AdjOR 2.3), older (AdjOR 1.6), slept less than 8 h (AdjOR 1.5), perceived increased anxiety (AdjOR 3.4), and adopted certain eating behaviors, e.g., comfort food consumption (AdjOR 2.0). According to free-text answers, the main reasons for ED were “school pressure”, “relationships with family and peers”, “negative emotional states” and “the pandemic”. The results show a high level of ED among adolescents, and the reported reasons may help to better address their psychological needs after the pandemic.
Public Rental Housing and Long-Term Settlement Intention of the Migrants in China: The Mediating Effect of Identity
Authors: Du Cuicui , Lou Wenlong , Qiao Yuhua , Zhang Yongchao
Published: September 2024

The urban settlement of migrants and their families is an important aspect of new urbanization. Affordable housing, a key measure to improve their living conditions, can advance their urbanization goals. Based on the China Migrants Dynamic Survey (CMDS) data and land transaction data of cities, this study employs a complementary log–log model to estimate the effect of public rental housing (PRH) on the long-term settlement intention (LTSI) of migrants and delves into the intrinsic effect mechanism through the mediating effect. The results show that: (1) Living in PRH can significantly improve the LTSI of migrants who rent. A series of robustness tests and endogeneity tests support the validity of this conclusion; (2) The visualization of a heterogeneity analysis shows that PRH has a greater influence on the LTSI of first-generation migrants and urban–urban migrants. As the city class of the destination decreases, the effect of PRH gradually diminishes; (3) A mechanism analysis suggests that a sense of identity plays a mediating role in PRH affecting the LTSI of the migrants, particularly in first-tier cities. This paper enriches the literature related to the field of housing security programs, provides policy references for enhancing the LTSI of the migrants, and promotes the development of urbanization.
Addition of Biochar to Green Roof Substrate to Enhance Plant Performance: A Long-Term Field Study
Authors: Nguyen Cuong Ngoc , Chau Hing-Wah , Muttil Nitin
Published: September 2024

Green roofs (GRs) have been widely adopted as an effective Green Infrastructure (GI) practice in cities worldwide, offering ecosystem services such as stormwater management and reduction of the urban heat island effect. However, their widespread implementation is still limited by a lack of local research and uncertain research findings. As a result, the potential benefits of GRs often cannot justify their high investment costs. Previous studies have sought to enhance the effectiveness of GRs by evaluating new GR systems, such as integrating GRs with green walls, blue roofs, photovoltaic (PV) panels, radiant cooling systems, as well as the use of innovative materials in GR substrates. Biochar, a carbon-rich substrate additive, has been recently investigated. The addition of biochar improves water/nutrient retention of GRs, thereby increasing substrate fertility and promoting plant performance. Although studies have examined the effects of biochar on GR plant growth, long-term observational studies focusing on the impacts of various biochar-related parameters remain necessary. Therefore, this research aims to assess the performance of GR plants with different biochar parameters, namely, amendment rates, application methods, and particle sizes. A one-year-long observational data of plant height, coverage area, and dry weight from six GR test beds was collected and analyzed. Results demonstrate the positive impacts of biochar on plant growth in different biochar-GR setups and types of plant species (wallaby grass, common everlasting, and billy buttons). The GR with medium biochar particles at the amendment rate of 15% v/v had the best plant performance. This contributes to increasing the feasibility of GRs by maximizing GR benefits to buildings where they are installed while reducing GR costs of irrigation and maintenance. The conclusions were further supported by observed data indicating reduced substrate temperature, which in turn reduces building energy consumption. Since vegetation is crucial in determining the effectiveness of a GR system, this study will offer valuable insights to GR designers and urban planners for developing optimal biochar-amended GR systems. Such systems provide numerous benefits over traditional GRs, including enhanced plant growth, reduced building energy costs, a shorter payback period, and reduced structural requirements.
Seismic Energy Dissipation and Hysteresis Performances of Distinctly Shaped Steel-Reinforced Concrete Column–Beam Joints under Cyclic Loading
Authors: Duan Junquan , Yang Delei , Liu Xiaochun , Xiang Ping
Published: September 2024

The distinctly shaped steel-reinforced concrete (SRC) column–beam framing system offers an innovative and tailored structural solution that combines load-bearing capabilities with architectural esthetics. This study introduces an innovative joint design methodology, focusing on examining the seismic responsiveness of the uniquely designed SRC columns when interconnected with reinforced concrete (RC) beams, subjected to bidirectional low cycle loading patterns through precisely calibrated pseudo-static evaluations with varied stirrup spacing. A comparative assessment was undertaken, comparing the joints of SRC test specimens with their RC counterparts, ensuring equivalency in steel and reinforcement area to maintain fairness. The evaluation encompassed a thorough examination of hysteresis loop backbone curves, as well as load–strain hysteresis patterns. It was found that the specimens incorporating structural steel and tubes demonstrated enhanced energy dissipation capabilities, surpassing other specimens in this critical performance aspect. An in-depth analysis was also conducted by comparing the ductility coefficient and the equivalent viscous damping coefficient to evaluate the joints’ performance in dissipating energy, coupled with a thorough examination of their stiffness deterioration behavior. The conclusion is that the energy dissipation capacity and stiffness degradation of distinctly shaped SRC column joints are superior to those of conventional, distinctly shaped concrete column joints, indicating promising application prospects.
Pounding Risk Assessment through Soil–Structure Interaction Analysis in Adjacent High-Rise RC Structures
Authors: Ebadi-Jamkhaneh Mehdi
Published: September 2024

This study investigates the seismic response of two 20-story adjacent reinforced concrete structures with differing lateral load-bearing systems, emphasizing the influence of soil–structure interaction. In total, 72 numerical models explored the combined effects of 9 earthquake motions, 4 soil types, and 2 structural designs. Analytical fragility curves revealed superior seismic resilience for the structure with shear walls compared to the bare frame structure. Shear walls increased the capacity to withstand earthquakes by up to 56% for each damage level. Soil behavior analysis investigated the effect of soil properties. Softer soil exhibited larger deformations and settlements compared to stiffer soil, highlighting soil ductility’s role in the system’s response. The study further assessed potential pounding between structures. The connection between structural stiffness and soil deformability significantly affected pounding risk. The provided gap (350 mm) proved insufficient to prevent pounding under various earthquake scenarios and soil types, leading to damage to RC components. These findings emphasize the crucial need to consider both structural systems and soil properties in seismic assessments.
Study on Mechanical Properties of Steel Fiber-Reinforced Geopolymer Concrete under Multi-Axial Loads
Authors: Wu Pengtao , Wan Xuan , Lu Qun , Zhang Yan
Published: September 2024

Steel fiber-reinforced geopolymer concrete (SFRGPC) is an inorganic cementitious material with environmentally friendly features. As compared to conventional concrete, SFRGPC has greater strength and durability, but it is brittle, making it similar to ordinary concrete. To date, the triaxial mechanical properties that regulate SFRGPC’s structural performance at serviceable and ultimate-limit conditions remain poorly understood. In this study, we conducted experimental and theoretical analyses of these properties. Conventional triaxial testing is used to investigate the effects of varying steel fiber contents and ratio of length to diameter under different confinement pressures on SFRGPC’s mechanical properties. The failure mode, maximal strength, stress–strain curve, maximum strain, and compressive toughness were analyzed and discussed. Under uniaxial compression, the failure mode of the SFRGPC specimens was a longitudinal split failure. The brittleness of the SFRGPC can be eliminated, and its resistance to breaking can be greatly improved by increasing the volume of steel fibers and the confining pressure in the mixture. The steel fiber content and ratio of length to diameter have obvious influence on the compressive strength of SFRGPC. As the steel fiber content increased, the compressive strength increased by 1.15–1.44 times; as the ratio of length to diameter increased, the compressive strength increased by 1.21–1.70 times. The increase in confining pressure can improve the compressive strength of concrete. With the increase in confining pressure, the increase trend of compressive strength becomes smooth. The confining pressure, steel fiber content, and steel fiber length have substantial influences on the compressive toughness index ηc3. Under increasing confining pressure, ηc3 increases linearly; however, after confining pressure is higher than 5 MPa, ηc3 tends toward a steady state when the confining pressure increases. Using numerical simulation, we also investigated the size effect of SFRGPC under triaxial load. The concrete cylinder’s strength does not significantly decrease as its size increases.
Cisplatin Resistance and Metabolism: Simplification of Complexity
Authors: Pervushin Nikolay V. , Yapryntseva Maria A. , Panteleev Mikhail A. , Zhivotovsky Boris , Kopeina Gelina S.
Published: September 2024

Cisplatin is one of the most well-known anti-cancer drugs and has demonstrated efficacy against numerous tumor types for many decades. However, a key challenge with cisplatin, as with any chemotherapeutic agent, is the development of resistance with a resultant loss of efficacy. This resistance is often associated with metabolic alterations that allow insensitive cells to divide and survive under treatment. These adaptations could vary greatly among different tumor types and may seem questionable and incomprehensible at first glance. Here we discuss the disturbances in glucose, lipid, and amino acid metabolism in cisplatin-resistant cells as well as the roles of ferroptosis and autophagy in acquiring this type of drug intolerance.
Pre-Clinical Models for CAR T-Cell Therapy for Glioma
Authors: Vandecandelaere Gust , Ramapriyan Rishab , Gaffey Matthew , Richardson Leland Geoffrey , Steuart Samuel Jeffrey , Tazhibi Masih , Kalaw Adrian , Grewal Eric P. , Sun Jing , Curry William T. , Choi Bryan D.
Published: September 2024

Immunotherapy represents a transformative shift in cancer treatment. Among myriad immune-based approaches, chimeric antigen receptor (CAR) T-cell therapy has shown promising results in treating hematological malignancies. Despite aggressive treatment options, the prognosis for patients with malignant brain tumors remains poor. Research leveraging CAR T-cell therapy for brain tumors has surged in recent years. Pre-clinical models are crucial in evaluating the safety and efficacy of these therapies before they advance to clinical trials. However, current models recapitulate the human tumor environment to varying degrees. Novel in vitro and in vivo techniques offer the opportunity to validate CAR T-cell therapies but also have limitations. By evaluating the strengths and weaknesses of various pre-clinical glioma models, this review aims to provide a roadmap for the development and pre-clinical testing of CAR T-cell therapies for brain tumors.
Inhibition of KDM2/7 Promotes Notochordal Differentiation of hiPSCs
Authors: Diaz-Hernandez Martha , Murakami Kimihide , Murata Shizumasa , Khan Nazir , Shenoy Sreekala , Henke Katrin , Yamada Hiroshi , Drissi Hicham
Published: September 2024

Intervertebral disc disease (IDD) is a debilitating spine condition that can be caused by intervertebral disc (IVD) damage which progresses towards IVD degeneration and dysfunction. Recently, human pluripotent stem cells (hPSCs) were recognized as a valuable resource for cell-based regenerative medicine in skeletal diseases. Therefore, adult somatic cells reprogrammed into human induced pluripotent stem cells (hiPSCs) represent an attractive cell source for the derivation of notochordal-like cells (NCs) as a first step towards the development of a regenerative therapy for IDD. Utilizing a differentiation method involving treatment with a four-factor cocktail targeting the BMP, FGF, retinoic acid, and Wnt signaling pathways, we differentiate CRISPR/Cas9-generated mCherry-reporter knock-in hiPSCs into notochordal-like cells. Comprehensive analysis of transcriptomic changes throughout the differentiation process identified regulation of histone methylation as a pivotal driver facilitating the differentiation of hiPSCs into notochordal-like cells. We further provide evidence that specific inhibition of histone demethylases KDM2A and KDM7A/B enhanced the lineage commitment of hiPSCs towards notochordal-like cells. Our results suggest that inhibition of KDMs could be leveraged to alter the epigenetic landscape of hiPSCs to control notochord-specific gene expression. Thus, our study highlights the importance of epigenetic regulators in stem cell-based regenerative approaches for the treatment of disc degeneration.
HOTAIR Promotes the Hyperactivation of PI3K/Akt and Wnt/β-Catenin Signaling Pathways via PTEN Hypermethylation in Cervical Cancer
Authors: Trujano-Camacho Samuel , Cantú-de León David , Pérez-Yepez Eloy , Contreras-Romero Carlos , Coronel-Hernandez Jossimar , Millan-Catalan Oliver , Rodríguez-Dorantes Mauricio , López-Camarillo Cesar , Gutiérrez-Ruiz Concepción , Jacobo-Herrera Nadia , Pérez-Plasencia Carlos
Published: September 2024

The mechanisms underlying the sustained activation of the PI3K/AKT and Wnt/β-catenin pathways mediated by HOTAIR in cervical cancer (CC) have not been extensively described. To address this knowledge gap in the literature, we explored the interactions between these pathways by driving HOTAIR expression levels in HeLa cells. Our findings reveal that HOTAIR is a key regulator in sustaining the activation of both signaling pathways. Specifically, altering HOTAIR expression—either by knockdown or overexpression—significantly influenced the transcriptional activity of the PI3K/AKT and Wnt/β-catenin pathways. Additionally, we discovered that HIF1α directly induces HOTAIR transcription, which in turn leads to the epigenetic silencing of the PTEN promoter via DNMT1. This process leads to the sustained activation of both pathways, highlighting a novel regulatory axis involving HOTAIR and HIF1α in cervical cancer. Our results suggest a new model in which HOTAIR sustains reciprocal activation of the PI3K/AKT and Wnt/β-catenin pathways through the HOTAIR/HIF1α axis, thereby contributing to the oncogenic phenotype of cervical cancer.
Investigating the Phytochemical Composition, Antioxidant, and Anti-Inflammatory Potentials of Cassinopsis ilicifolia (Hochst.) Kuntze Extract against Some Oxidative Stress and Inflammation Molecular Markers
Authors: Mfotie Njoya Emmanuel , McGaw Lyndy J. , Makhafola Tshepiso J.
Published: September 2024

Oxidative stress is a key factor that activates several transcription factors and mediators involved in the inflammatory pathways responsible for the pathogenesis of many chronic diseases. Targeting the expression of these mediators represents a promising approach to preventing these diseases. Cassinopsis ilicifolia leaf infusion is traditionally used for treating conditions such as inflammation and pain relief. Thus, the present study assessed the antioxidant and anti-inflammatory activities of the hydroethanolic leaf extract of C. ilicifolia using in vitro and cell-based assays. As a result, C. ilicifolia extract exhibited the highest DPPH• and ABTS•+ radical scavenging potential. At the same time, it weakly scavenged the Fe3+-TPTZ radical up to 200 µg/mL, thus suggesting a different antioxidant mechanism triggered during each assay. Additionally, C. ilicifolia extract inhibited NO production and 15-LOX activity with IC50 values of 21.10 µg/mL and 40.28 µg/mL, respectively. Further, C. ilicifolia extract was found to strongly inhibit ROS production in LPS-activated RAW 264.7 cells, and the study of its mechanism of action showed that it exerts its anti-inflammatory effect by downregulating the expression of inflammatory mediators such as IL-1β, TNF-α, and COX-2. Overall, C. ilicifolia extract showed consistent potency in all assays, and the analysis of its phytochemical profile led to the identification of 30 compounds, among which the most abundant were secologanic acid (1), chlorogenic acid (3CQA) (2), monotropein (3), chlorogenic acid (5CQA) (4), geniposidic acid (5), rutin (6), quercetin 3-galactoside (7), astragalin-7-rhamnoside (8), and minecoside (9) that are possibly responsible for its anti-inflammatory and antioxidant activities. Therefore, our findings suggested the potential use of C. ilicifolia as an alternative source for developing plant-based products against oxidative stress and inflammation-related conditions.
Ferredoxins: Functions, Evolution, Potential Applications, and Challenges of Subtype Classification
Authors: Syed Khajamohiddin
Published: September 2024

Ferredoxins are proteins found in all biological kingdoms and are involved in essential biological processes including photosynthesis, lipid metabolism, and biogeochemical cycles. Ferredoxins are classified into different groups based on the iron-sulfur (Fe-S) clusters that they contain. A new subtype classification and nomenclature system, based on the spacing between amino acids in the Fe-S binding motif, has been proposed in order to better understand ferredoxins’ biological diversity and evolutionary linkage across different organisms. This new classification system has revealed an unparalleled diversity between ferredoxins and has helped identify evolutionarily linked ferredoxins between species. The current review provides the latest insights into ferredoxin functions and evolution, and the new subtype classification, outlining their potential biotechnological applications and the future challenges in streamlining the process.
Unraveling the Mechanisms of S100A8/A9 in Myocardial Injury and Dysfunction
Authors: Xu Yuanbo , Wang Yixuan , Ning Ke , Bao Yimin
Published: September 2024

S100A8 and S100A9, which are prominent members of the calcium-binding protein S100 family and recognized as calprotectin, form a robust heterodimer known as S100A8/A9, crucial for the manifestation of their diverse biological effects. Currently, there is a consensus that S100A8/A9 holds promise as a biomarker for cardiovascular diseases (CVDs), exerting an influence on cardiomyocytes or the cardiovascular system through multifaceted mechanisms that contribute to myocardial injury or dysfunction. In particular, the dualistic nature of S100A8/A9, which functions as both an inflammatory mediator and an anti-inflammatory agent, has garnered significantly increasing attention. This comprehensive review explores the intricate mechanisms through which S100A8/A9 operates in cardiovascular diseases, encompassing its bidirectional regulatory role in inflammation, the initiation of mitochondrial dysfunction, the dual modulation of myocardial fibrosis progression, and apoptosis and autophagy. The objective is to provide new information on and strategies for the clinical diagnosis and treatment of cardiovascular diseases in the future.
Exogenous Melatonin Modulates Photosynthesis and Antioxidant Systems for Improving Drought Tolerance of Sorghum Seedling
Authors: Bo Yushan , Xing Yifan , Wang Yu , Gu Wendong , Jiang Xinyi , Yu Jiarui , Shi Xiaolong , Liu Chunjuan , Liu Chang , Zhou Yufei
Published: September 2024

Sorghum faces significant production challenges due to drought stress. Melatonin has been demonstrated to play a crucial role in coping with stresses in plants. This study investigated the effect of exogenous melatonin on the sorghum seedling growth, photosynthetic capacity, and antioxidant system under drought stress. The results indicated that drought stress inhibited the growth of sorghum seedlings by a marked reduction in leaf relative water content, along with a significant increase in both malondialdehyde and hydrogen peroxide content. The drought stress also led to a significant diminution in chlorophyll contents, thereby curtailing the capacity for light energy capture. Furthermore, the efficiency of the photosynthetic electron transport chain was adversely impacted. However, the application of exogenous melatonin notably mitigated the adverse effects on sorghum seedlings under the drought stress. Additionally, it stimulated an elevation in the photosynthetic rate and a decrease in non-photochemical quenching. The exogenous melatonin also facilitated the preservation of the chloroplast ultra-structure and boosted the activity of antioxidant enzymes and the content of non-enzymatic antioxidants. Cluster heat maps and principal component analysis further revealed significant correlations among various parameters under different treatment conditions. These results highlight melatonin’s role in improving sorghum’s drought tolerance, which is beneficial for agricultural management.
Development of an Indirect ELISA to Distinguish between Porcine Sapelovirus-Infected and -Vaccinated Animals Using the Viral Nonstructural Protein 3AB
Authors: Zhong Zuchang , Li Benqiang , Tao Jie , Cheng Jinghua , Shi Ying , Tang Pan , Jiao Jiajie , Liu Huili
Published: September 2024

Porcine sapelovirus (PSV) is a new pathogen that negatively impacts the pig industry in China. Affected pigs experience severe diarrhea and even death. Vaccination is used to control disease outbreaks, and sensitive diagnostic methods that can distinguish infected animals from vaccinated animals (DIVA) are essential for monitoring the effectiveness of disease control programs. Tests based on the detection of the nonstructural protein (NSP) 3AB are reliable indicators of viral replication in infected and vaccinated animals. In this study, the recombinant PSV 3AB protein was expressed by a prokaryotic expression system, and an indirect ELISA method was established. Serum samples from healthy animals, immunized animals, and infected animals were evaluated. The ELISA method identified 3AB with high sensitivity (99.78%) and specificity (100.0%), and no cross-reaction was observed with serum antibodies against porcine reproductive and respiratory syndrome virus (PRRSV), infection with classical swine fever virus (CSFV), pseudorabies virus (PRV), bovine viral diarrhea virus (BVDV), porcine epidemic diarrhea virus (PEDV), or foot-and-mouth disease virus (FMDV). The ELISA method described here can effectively distinguish infected and vaccinated animals and is an important inexpensive tool for monitoring serum and controlling PSV.
Robotic-Assisted Radical Cystectomy: Single-Center Analysis of Factors Impacting Clavien ≥ II Complications
Authors: Sighinolfi Maria Chiara , Calcagnile Tommaso , Panio Enrico , Assumma Simone , Sarchi Luca , Sandri Marco , Santangelo Emanuela , Petix Michele , Sangalli Mattia , Turri Filippo , Bozzini Giorgio , Moschovas Marcio Covas , Patel Vipul , Rocco Bernardo
Published: September 2024

Despite the advent of robotics and the decreasing rate of complications after radical cystectomy, several factors are renowned to impair the early outcomes of this procedure. The aim of this paper is to provide a multivariate analysis (MVA) of patient and surgical procedure-related variables likely to affect postoperative course and 30-day complication rate. Fifty-five robotic-assisted radical cystectomies (RARCs) performed at a single center from July 2021 to March 2023 were enrolled. Baseline demographics, comorbidities, and intraoperative and postoperative data were collected. Uni- and multivariate analyses were performed to evaluate the relationship with Clavien ≥ II complications arising within 30 days of surgery. A postoperative Clavien ≥ II complication was evident in 15 patients (28%), whereas Clavien ≥ III occurred only in 5 (9%). At MVA, the only independent predictor of Clavien ≥ II complications was a prior neoadjuvant chemotherapy (OR 5.6; 95% CI 1.22–25.3, p = 0.026). Recognized the small sample size, patients who received a prior NAC should deserve special care within the postoperative course.
MnBi2Te4 Thin-Film Photodetector with a Millisecond Response Speed and Long-Term Air Stability
Authors: Yang Ming , Ren Haotian , Deng Wenze , Chang Haoliang , Li Qiqin , Zhou Hongxi , Tu Xiaoguang , Zhong Mian , Li Fei , Zhu Xinyu
Published: September 2024

Topological materials with well-defined surfaces and edges have become a prominent research topic. As topological insulators, MnBi2Te4 thin films, with their unique surfaces, exhibit exceptional electron transport properties and good applicability in low-noise, high-sensitivity photoelectric detection. This paper reports a straightforward, efficient, and cost-effective thermal evaporation method for preparing quantum MnBi2Te4 thin films, along with an investigation into their photoelectric detection performance. These films can be used to fabricate array devices, with the resulting photodetectors achieving a response current of 97 mA W−1 at room temperature and a response speed of <1 ms. Moreover, they demonstrate stability in the air for >30 d, with the photoelectric performance degrading by <15%. Our research introduces a new application for topological materials in photoelectric detection and establishes a strong foundation for the design and development of high-performance photodetectors in the future.
The Role of the Manganese Content on the Properties of Mn3O4 and Reduced Graphene Oxide Nanocomposites for Supercapacitor Electrodes
Authors: Fernández-Jiménez Víctor , de Bernardi-Martín Santiago , García-Gómez Alejandra , López-Díaz David , Sánchez-Montero M. Jesús , Velázquez M. Mercedes , Merchán M. Dolores
Published: September 2024

Increasing the energy density and power of supercapacitors through hybrids of carbonaceous materials and metal oxides continues to be the subject of numerous research works. The correlation between specific capacitance and the properties of materials used as electrodes attracts great interest. In the present study, we investigated composites (GO/Mn3O4) prepared by the hydrothermal method with a variable ratio of GO/Mn3O4 and tested them as supercapacitor electrode materials in three- and two-electrode cells. The chemical characterization carried out by X-ray photoelectron spectroscopy and the adsorption techniques used allowed the determination of the surface carbon and oxygen content, as well as its textural properties. In this work, we analyzed the contribution of the double layer and the Faradaic reactions to the value of the final capacitance of the synthesized materials. Beyond empirically obtaining the electrochemical properties, these have been related to the physicochemical characteristics of the hybrids to help design materials with the best performance for supercapacitor electrodes.
Ultrasound-Assisted Acellular Spinal Cord Scaffold for Spinal Cord Injury Treatment
Authors: Deng Xi , Liu Yun , Xu Zhongsheng , Yin Hong
Published: September 2024

Spinal cord injury (SCI) treatment remains challenging globally, with limited breakthroughs. Tissue engineering offers promise, particularly using acellular spinal cord scaffolds. This study developed a 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC)-crosslinked vascular endothelial growth factor (VEGF)-modified acellular spinal cord scaffold for sustained VEGF release. The results show sustained VEGF release over 20 days without altering the scaffold’s properties. Enhanced stability and mechanical properties were observed without increased cytotoxicity. In a rat SCI model, the system improved motor function, reduced glial scarring, and restored spinal cord morphology and histology, indicating potential for SCI therapy.
Study on the Alloying Elements Competition Mechanism of Nix1Crx2Cox3Al15Ti10 Alloys Based on High-Throughput Computation and Numerical Analysis
Authors: Liu Yu , Wang Lijun , He Wenjie , Liu Yunpeng
Published: September 2024

Previous studies on the physical properties of alloy materials often focus solely on analyzing the impact of individual alloying element content, overlooking the underlying mechanism behind the synergistic action of multiple alloying elements. Therefore, in this study, we propose a combination of high-throughput computation and numerical analysis to conduct single-element (SE) analysis and multi-element (ME) analysis on the internal relationships between alloying element content and physical properties for the multi-component Nix1Crx2Cox3Al15Ti10 alloys, aiming to elucidate the competition mechanism among the Ni, Cr, and Co elements. The analysis of SE reveals how the physical properties of alloys are affected by the content of each individual alloying element, and the ME analysis further unveils the underlying competitive relationships among multiple alloying elements. The order of competitive intensity for the formation of lattice constant is Cr > Co > Ni, whereas for the formation of elastic constants and elastic moduli it is Ni > Co > Cr. At the same time, there are contradictory conclusions, such as the SE analysis showing that the Ni content is positively correlated with elastic constant C11, while the ME analysis demonstrates that the Ni element produces a negative competitive direction. This outcome arises from the omission of considering the combined impacts of various alloying elements in SE analysis. Therefore, the ME analysis can compensate for the limitations of SE analysis, and the integration of these two analytical methods is more conducive to elucidating the competition mechanism among various alloying elements in shaping the physical properties of alloys, which provides a promising avenue for theoretical research.
Transport Behavior of Paranitroaniline through a Flat-Sheet Supported Liquid Membrane Using Tributylphosphate as a Carrier
Authors: Algreiby Azizah , Alharbi Lama , Kouki Noura , Tar Haja , Alnafisah Abrar , Béji Lotfi
Published: September 2024

4-Nitroaniline (PNA) is a toxic organic compound commonly found in wastewater, posing significant environmental concerns due to its toxicity and potential carcinogenicity. In this study, the recovery of PNA from aqueous solutions was investigated using a supported liquid membrane (SLM). The membrane, which consists of polypropylene Celgard 2500 (PP-Celg), was embedded with the extractant tributyl phosphate (TBP). Various factors influencing the efficiency of PNA transportation were studied, including the concentration of PNA in the source phase, pH of the source phase, NaOH concentration in the receiving phase, and choice of stripping agents. Optimal conditions for the experiment were determined to be a source phase PNA concentration of 20 ppm at pH 7, distilled water as the receiving phase, TBP as the carrier in the organic phase, and a transport time of 8 h. The extraction process was conducted under ambient temperature and pressure conditions, yielding results indicative of a first-order linearized reaction. Additionally, membrane stability and liquid membrane loss were evaluated.
Mössbauer Studies of Haltern 70 Amphorae from Castro do Vieito, Northwest of Portugal
Authors: Costa Benilde F. O. , Wagner Friedrich E. , Häusler Werner , Stieghorst Christian , Silva António José Marques da
Published: September 2024

Haltern 70 amphorae sherds from Castro do Vieito, a Roman settlement from the NW of Portugal occupied during the early imperial period, were studied by Mössbauer spectroscopy at room temperature and 4.2 K, XRD, and XRF, aiming to understand the firing conditions of their production. Firing in air at 750 and 800 °C were performed in a sherd that was carefully studied. Also, a handle with part of the neck attached and with the potter’s stamp “LH …” was studied. In general, it can be deduced that the amphorae were fired under reducing conditions between 800 and 950 °C, having been subjected to an oxidation process only when already cooling. It was also inferred that the provenance of all the Haltern 70 amphorae found in Castro do Vieito is probably the same and that the stamped amphora also seems to come from the same locality.
All-Optical Switching Using Cavity Modes in Photonic Crystals Embedded with Hyperbolic Metamaterials
Authors: Liu Chang , Wei Dong , Lin Xiaochun , Zheng Yaoxian
Published: September 2024

Hyperbolic metamaterials (HMMs) are highly anisotropic materials with the unique property of generating electromagnetic modes. Understanding how these materials can be applied to control the propagation of light waves remains a major focus in photonics. In this study, we inserted a finite-size HMM rod into the point defect of two-dimensional photonic crystals (PhCs) and investigated the unique cavity modes of this hybrid system. The HMM enhances the efficiency of the cavity system in controlling light transmission. Numerical results demonstrate that the cavity modes based on HMMs can be categorized into various types, showing high Q-factors and promising potential for resonant modulation. Furthermore, the switching performance of the cavity with an HMM rod was examined, revealing that the finite-size HMM modes are highly frequency-sensitive and suitable for nonlinear controlled all-optical switching. These switches, characterized by low power consumption and high extinction ratios, are highly suitable for integration into photonic systems. Our investigation on the new type of HMM cavity illustrates that anisotropic materials can be effectively applied in cavity systems to generate highly efficient modes for filtering and switching.
Model for End-Stage Liver Disease Including Na, Age, and Sex Is Powerful Predictor of Survival in COVID-19 Patients on Extracorporeal Membrane Oxygenation
Authors: Jenkins Freya Sophie , Morjan Mohammed , Minol Jan-Philipp , Yilmaz Esma , Dalyanoglu Ismail , Immohr Moritz Benjamin , Korbmacher Bernhard , Boeken Udo , Lichtenberg Artur , Dalyanoglu Hannan
Published: September 2024

Aim: Extracorporeal membrane oxygenation (ECMO) is resource-intensive, is associated with significant morbidity and mortality, and requires careful patient selection. This study examined whether the model for end-stage liver disease (MELD) score is a suitable predictor of in-hospital mortality in patients with COVID-19. Materials and Methods: We retrospectively assessed patients with COVID-19 on ECMO at our institution from March 2020 to May 2021. MELD scoring was performed using laboratory values recorded prior to ECMO initiation. A multiple logistic regression model was established. Results: A total of 66 patients with COVID-19 on ECMO were included (median age of 58.5 years; 83.3% male). The in-hospital mortality was 74.2%. In relation to mortality, patients with MELD Na scores >13.8 showed 6.5-fold higher odds, patients aged >53.5 years showed 18.4-fold higher odds, and male patients showed 15.9-fold higher odds. The predictive power of a model combining the MELD Na with age and sex was significant (AUC = 0.883, p < 0.001). The findings in the COVID-19 patients were not generalizable to a group of non-COVID-19 patients on ECMO. Conclusions: A model combining the MELD Na, age, and sex has high predictive power for in-hospital mortality in patients with COVID-19 on ECMO, and it may be clinically useful for guiding patient selection in critically ill COVID-19 patients both now and in the future, should the virus widely re-emerge.
Improving Shape-Sensing Robotic-Assisted Bronchoscopy Outcomes with Mobile Cone-Beam Computed Tomography Guidance
Authors: Bashour Sami I. , Khan Asad , Song Juhee , Chintalapani Gouthami , Kleinszig Gerhard , Sabath Bruce F. , Lin Julie , Grosu Horiana B. , Jimenez Carlos A. , Eapen Georgie A. , Ost David E. , Sarkiss Mona , Casal Roberto F.
Published: September 2024

Background: Computed tomography to body divergence (CTBD) is one of the main barriers to bronchoscopic techniques for the diagnosis of peripherally located lung nodules. Cone-beam CT (CBCT) guidance is being rapidly adopted to correct for this phenomenon and to potentially increase diagnostic outcomes. In this trial, we hypothesized that the addition of mobile CBCT (m-CBCT) could improve the rate of tool in lesion (TIL) and the diagnostic yield of shape-sensing robotic-assisted bronchoscopy (SS-RAB). Methods: This was a prospective, single-arm study, which enrolled patients with peripheral lung nodules of 1–3 cm and compared the rate of TIL and the diagnostic yield of SS-RAB alone and combined with mCBCT. Results: A total of 67 subjects were enrolled, the median nodule size was 1.7 cm (range, 0.9–3 cm). TIL was achieved in 23 patients (34.3%) with SS-RAB alone, and 66 patients (98.6%) with the addition of mCBCT (p < 0.0001). The diagnostic yield of SS-RAB alone was 29.9% (95% CI, 29.3–42.3%) and it was 86.6% (95% CI, 76–93.7%) with the addition of mCBCT (p < 0.0001). There were no pneumothoraxes or any bronchoscopy-related complications, and the median total dose–area product (DAP) was 50.5 Gy-cm2. Conclusions: The addition of mCBCT guidance to SS-RAB allows bronchoscopists to compensate for CTBD, leading to an increase in TIL and diagnostic yield, with acceptable radiation exposure.
Pre-Reconstruction Processing with the Cycle-Consist Generative Adversarial Network Combined with Attention Gate to Improve Image Quality in Digital Breast Tomosynthesis
Authors: Gomi Tsutomu , Ishihara Kotomi , Yamada Satoko , Koibuchi Yukio
Published: September 2024

The current study proposed and evaluated “residual squeeze and excitation attention gate” (rSEAG), a novel network that can improve image quality by reducing distortion attributed to artifacts. This method was established by modifying the Cycle Generative Adversarial Network (cycleGAN)-based generator network using projection data for pre-reconstruction processing in digital breast tomosynthesis. Residual squeeze and excitation were installed in the bridge of the generator network, and the attention gate was installed in the skip connection between the encoder and decoder. Based on the radiation dose index (exposure index and division index) incident on the detector, the cases approved by the ethics committee and used for the study were classified as reference (675 projection images) and object (675 projection images). For the cases, unsupervised data containing a mixture of cases with and without masses were used. The cases were trained using cycleGAN with rSEAG and the conventional networks (ResUNet and U-Net). For testing, predictive processing was performed on cases (60 projection images) that were not used for learning. Images were generated using filtered backprojection reconstruction (kernel: Ramachandran and Lakshminarayanan) from projection data for testing data and without pre-reconstruction processing data (evaluation: in-focus plane). The distortion was evaluated using perception-based image quality evaluation (PIQE) analysis, texture analysis (feature: “Homogeneity” and “Contrast”), and a statistical model with a Gumbel distribution. PIQE has a low rSEAG value. Texture analysis showed that rSEAG and a network without cycleGAN were similar in terms of the “Contrast” feature. In dense breasts, ResUNet had the lowest “Contrast” feature and U-Net had differences between cases. The maximal variations in the Gumbel plot, rSEAG reduced the high-frequency ripple artifacts. In this study, rSEAG could improve distortion and reduce ripple artifacts.