bluuebunny/crossref_metadata_2025 huggingface dataset:
Respiratory viruses interacting with cells: the importance of electrostatics
Authors: Lauster Daniel , Osterrieder Klaus , Haag Rainer , Ballauff Matthias , Herrmann Andreas
Published: June 2023

The COVID-19 pandemic has rekindled interest in the molecular mechanisms involved in the early steps of infection of cells by viruses. Compared to SARS-CoV-1 which only caused a relatively small albeit deadly outbreak, SARS-CoV-2 has led to fulminant spread and a full-scale pandemic characterized by efficient virus transmission worldwide within a very short time. Moreover, the mutations the virus acquired over the many months of virus transmission, particularly those seen in the Omicron variant, have turned out to result in an even more transmissible virus. Here, we focus on the early events of virus infection of cells. We review evidence that the first decisive step in this process is the electrostatic interaction of the spike protein with heparan sulfate chains present on the surface of target cells: Patches of cationic amino acids located on the surface of the spike protein can interact intimately with the negatively charged heparan sulfate chains, which results in the binding of the virion to the cell surface. In a second step, the specific interaction of the receptor binding domain (RBD) within the spike with the angiotensin-converting enzyme 2 (ACE2) receptor leads to the uptake of bound virions into the cell. We show that these events can be expressed as a semi-quantitative model by calculating the surface potential of different spike proteins using the Adaptive Poison-Boltzmann-Solver (APBS). This software allows visualization of the positive surface potential caused by the cationic patches, which increased markedly from the original Wuhan strain of SARS-CoV-2 to the Omicron variant. The surface potential thus enhanced leads to a much stronger binding of the Omicron variant as compared to the original wild-type virus. At the same time, data taken from the literature demonstrate that the interaction of the RBD of the spike protein with the ACE2 receptor remains constant within the limits of error. Finally, we briefly digress to other viruses and show the usefulness of these electrostatic processes and calculations for cell-virus interactions more generally.
Inference of drowning sites using bacterial composition and random forest algorithm
Authors: Su Qin , Yang Chengliang , Chen Ling , She Yiqing , Xu Quyi , Zhao Jian , Liu Chao , Sun Hongyu
Published: June 2023

Diagnosing the drowning site is a major challenge in forensic practice, particularly when corpses are recovered from flowing rivers. Recently, forensic experts have focused on aquatic microorganisms, including bacteria, which can enter the bloodstream during drowning and may proliferate in corpses. The emergence of 16S ribosomal RNA gene (16S rDNA) amplicon sequencing has provided a new method for analyzing bacterial composition and has facilitated the development of forensic microbiology. We propose that 16S rDNA amplicon sequencing could be a useful tool for inferring drowning sites. Our study found significant differences in bacterial composition in different regions of the Guangzhou section of the Pearl River, which led to differences in bacteria of drowned rabbit lungs at different drowning sites. Using the genus level of bacteria in the lung tissue of drowned rabbits, we constructed a random forest model that accurately predicted the drowning site in a test set with 100% accuracy. Furthermore, we discovered that bacterial species endemic to the water were not always present in the corresponding drowned lung tissue. Our findings demonstrate the potential of a random forest model based on bacterial genus and composition in drowned lung tissues for inferring drowning sites.
Association of N-nitrosodimethylamine exposure with cognitive impairment based on the clues of mice and humans
Authors: Liu Wei , Huang Jia , Yan Zhi , Lin Yankui , Huang Guanqin , Chen Xiao , Wang Zhou , Spencer Peter S. , Liu Jianjun
Published: June 2023

N-nitrosodimethylamine (NDMA) is an environmental and food contaminant, but limited data to concern whether NDMA has adverse effects on the brain. This study first determined the concentration of NDMA in foods from aquaculture markets in Shenzhen, then analyzed the effects on C57BL/6 mice and further evaluated on the urine samples of elderly Chinese residents with normal cognition (NC, n = 144), cognitive decline (CD, n = 116) and mild cognitive impairment (MCI, n = 123). The excessive rate of NDMA in foods was 3.32% (27/813), with a exceeding range of 4.78–131.00 μg/kg. Behavioral tests showed that 60 days treatment of mice with 3 mg/kg NDMA reduced cognitive performance. Cognitive impairment in human was significantly associated with sex, educational levels, length of residence in Shenzhen, household registration, passive smoking, rice, fresh vegetables, bacon products. NDMA was detected in 55.4% (212/383) of urine samples, with a median concentration of 0.23 μg/L (1.20 × 10 –7–157.39 μg/L). The median concentration for NC, CD and MCI were 0.32, 0.27, and 0 μg/L, respectively. The urinary NDMA concentration had a strong negative correlation with cognitive impairment (Kendall’s Tau-b = −0.89, P = 0.024). The median estimated daily intake (EDI) of NDMA was determined to be 6.63 ng/kg-bw/day. Taken together, there appears to be an association between NDMA and human and murine cognition, which provides a new clue to Alzheimer’s disease (AD).
Frequency, pattern and predictors of cognitive impairments in patients with Parkinson’s disease using the Community Screening Instrument for Dementia
Authors: Ogbimi Ewere Marie , Akemokwe Fatai Momodu , Ogunrin Olubunmi
Published: June 2023

Background

Parkinson’s disease (PD) is a chronic neurodegenerative disorder complicated by cognitive dysfunctions which are associated with increased caregiver burden, pressure on community health facilities, and mortality in affected patients. Most of the data concerning cognitive dysfunctions in PD are from studies conducted in Europe and North America, but there is paucity of data from Sub-Saharan Africa.

Objective

The objective of this study is to determine the frequency, pattern and predictors of cognitive impairments amongst patients with Parkinson’s disease.

Materials and methods

This was a cross sectional case control study carried out at a tertiary health facility in South-south Nigeria. Participants with PD were consecutively recruited from the neurology outpatient clinics. Demographic and disease-specific data were obtained with the use of a pre-tested questionnaire. Cognitive performance of thirty patients with PD were compared with thirty demographically matched controls using the Community Screening Instrument for Dementia (CSID). CSID was already validated among Nigerians.

Results

The frequency of cognitive impairment using the CSID was 50% for PD patients (3.3% for controls). Poor cognitive performance was observed across several cognitive domains including language, executive dysfunction, psychomotor speed, and constructional apraxia among PD patients. The independent predictors of the overall cognitive impairment in patients with PD determined by logistic regression analysis include recall deficiency (p = 0.007), impairment with naming (p = 0.044), apraxia (p = 0.003), Hoen&Yahr staging (p = 0.046), UPDRS score (p = 0.015) and age at presentation (p = 0.014).

Conclusion

Cognitive impairments occur more frequently in patients with PD compared to controls. This study also demonstrated the predictive role of severity of disease based on Hoehn &Yahr staging and UPDRS score, and presence of recall deficiency, poor naming ability and apraxia.
An open-source human-in-the-loop BCI research framework: method and design
Authors: Gemborn Nilsson Martin , Tufvesson Pex , Heskebeck Frida , Johansson Mikael
Published: June 2023

Brain-computer interfaces (BCIs) translate brain activity into digital commands for interaction with the physical world. The technology has great potential in several applied areas, ranging from medical applications to entertainment industry, and creates new conditions for basic research in cognitive neuroscience. The BCIs of today, however, offer only crude online classification of the user's current state of mind, and more sophisticated decoding of mental states depends on time-consuming offline data analysis. The present paper addresses this limitation directly by leveraging a set of improvements to the analytical pipeline to pave the way for the next generation of online BCIs. Specifically, we introduce an open-source research framework that features a modular and customizable hardware-independent design. This framework facilitates human-in-the-loop (HIL) model training and retraining, real-time stimulus control, and enables transfer learning and cloud computing for the online classification of electroencephalography (EEG) data. Stimuli for the subject and diagnostics for the researcher are shown on separate displays using web browser technologies. Messages are sent using the Lab Streaming Layer standard and websockets. Real-time signal processing and classification, as well as training of machine learning models, is facilitated by the open-source Python package Timeflux. The framework runs on Linux, MacOS, and Windows. While online analysis is the main target of the BCI-HIL framework, offline analysis of the EEG data can be performed with Python, MATLAB, and Julia through packages like MNE, EEGLAB, or FieldTrip. The paper describes and discusses desirable properties of a human-in-the-loop BCI research platform. The BCI-HIL framework is released under MIT license with examples at:bci.lu.se/bci-hil(or at:github.com/bci-hil/bci-hil).
Successful extracorporeal membrane oxygenation for postoperative cardiopulmonary failure in newborns with congenital diaphragmatic hernia: case reports and literature reviews
Authors: Hong Song-Ming , Chen Xiu-Hua , Zhou Si-Jia , Hong Jun-Jie , Zheng Yi-Rong , Chen Qiang , Huang Jin-Xi
Published: June 2023

Introduction

Congenital diaphragmatic hernia (CDH) is a structural defect caused by inadequate fusion of the pleuroperitoneal membrane that forms the diaphragm, allowing peritoneal viscera to protrude into the pleural cavity. Up to 30% of newborns with CDH require extracorporeal membrane oxygenation (ECMO) support. As with all interventions, the risks and benefits of ECMO must be carefully considered in these patients. Cardiopulmonary function has been shown to worsen rather than improve after surgical CDH repair. Even after a detailed perioperative assessment, sudden cardiopulmonary failure after surgery is dangerous and requires timely and effective treatments.

Method

Three cases of cardiopulmonary failure after surgical CDH treatment in newborns have been reported. ECMO support was needed for these three patients and was successfully discontinued. We report our treatment experience.

Conclusion

ECMO is feasible for the treatment of postoperative cardiopulmonary failure in newborns with CDH.
Role of endogenous and exogenous antioxidants in risk of six cancers: evidence from the Mendelian randomization study
Authors: Zhu Jiahao , Lian Jie , Wang Xin , Wang Ren , Pang Xiangyi , Xu Benjie , Wang Xing , Li Chenyang , Ji Shengjun , Lu Haibo
Published: June 2023

Background: Although oxidative stress is known to contribute to cancer, and endogenous and exogenous antioxidants are thought to prevent tumorigenesis by suppressing oxidative stress-induced DNA damage, antioxidants have also been reported to show negative effects on tumor formation, necessitating characterization of the causal associations between antioxidants and cancer risk.

Methods: In this study, Mendelian randomization (MR) analysis, primarily inverse-variance weighted MR, was used to assess the causal effect of six endogenous and five exogenous diet-derived antioxidants on the risk of six cancers. MR-Egger intercept test and Cochran’s Q statistic were utilized to assess pleiotropy and heterogeneity, respectively.

Results: For endogenous antioxidants, a bidirectional two-sample MR analysis was conducted. Our findings suggested that serum albumin has a negative causal association with the risk of prostate cancer [odds ratio (OR) = 0.78, 95% confidence interval (CI): 0.68–0.91, p = 0.001]. The risks of the six cancers showed no significant associations with endogenous antioxidants in the converse MR analysis. For exogenous antioxidants, the unidirectional two-sample MR analysis exhibited a nominal relationship between the serum retinol level and non-small-cell lung cancer risk (OR = 0.29, 95% CI: 0.11–0.76, p = 0.011).

Conclusions: Thus, our study revealed the protective effects of genetic susceptibility to high circulating albumin levels on prostate cancer, providing potential targeted interventions for prostate cancer prevention.
Oat beta-glucan reduces colitis by promoting autophagy flux in intestinal epithelial cells via EPHB6-TFEB axis
Authors: Xu Mingyang , Ling Fangmei , Li Junrong , Chen Yidong , Li Shuang , Cheng Yiyu , Zhu Liangru
Published: June 2023

Inflammatory bowel disease (IBD) is a group of chronic inflammatory disorders of the gastrointestinal tract, mainly including Crohn’s disease and ulcerative colitis. Epidemiological findings suggest that inadequate dietary fibers intake may be a risk factor for IBD. Oat beta-glucan is a type of fermentable dietary fiber and has been proved to reduce experimental colitis. However, the mechanism remains unclear. The aim of this study was to explore the role and possible mechanism of oat beta-glucan in reducing experimental colitis. We used a dextran sulfate sodium (DSS)-induced mice acute colitis model to explore the potential mechanism of oat beta-glucan in reducing experimental colitis. As a result, oat beta-glucan upregulated the expressions of Erythropoietin-producing hepatocyte receptor B6 (EPHB6) and transcription factor EB (TFEB), promoted autophagy flux and downregulated the expressions of interleukin 1 beta (IL-1β), interleukin 6 (IL-6) and tumor necrosis factor alpha (TNF-α) in intestinal epithelial cells (IECs). The role of the EPHB6-TFEB axis was explored using a lipopolysaccharide-induced HT-29 cells inflammation model. The results revealed that EPHB6 regulated the expression of TFEB, and knockdown of EPHB6 decreased the protein level of TFEB. When EPHB6 or TFEB was knocked down, autophagy flux was inhibited, and the anti-inflammatory effect of sodium butyrate, a main metabolite of oat beta-glucan in the gut, was blocked. In summary, our findings demonstrated that oat beta-glucan reduced DSS-induced acute colitis in mice, promoted autophagy flux via EPHB6-TFEB axis and downregulated the expressions of IL-1β, IL-6 and TNF-α in IECs, and this effect may be mediated by butyrate.
Assessment and comparison of rhizosphere communities in cultivated Vaccinium spp. provide a baseline for study of causative agents in decline
Authors: Kawash Joseph , Oudemans Peter V. , Erndwein Lindsay , Polashock James J.
Published: June 2023

It has long been recognized that the community of organisms associated with plant roots is a critical component of the phytobiome and can directly or indirectly contribute to the overall health of the plant. The rhizosphere microbial community is influenced by a number of factors including the soil type, the species of plants growing in those soils, and in the case of cultivated plants, the management practices associated with crop production. Vaccinium species, such as highbush blueberry and American cranberry, are woody perennials that grow in sandy, acidic soils with low to moderate levels of organic matter and a paucity of nutrients. When properly maintained, fields planted with these crops remain productive for many years. In some cases, however, yields and fruit quality decline over time, and it is suspected that degenerating soil health and/or changes in the rhizosphere microbiome are contributing factors. Determining the assemblage of bacterial and fungal microorganisms typically associated with the rhizosphere of these crops is a critical first step toward addressing the complex issue of soil health. We hypothesized that since blueberry and cranberry are in the same genus and grow in similar soils, that their associated rhizosphere microbial communities would be similar to each other. We analyzed the eukaryotic (primarily fungal) and bacterial communities from the rhizosphere of representative blueberry and cranberry plants growing in commercial fields in New Jersey. The data presented herein show that while the bacterial communities between the crops is very similar, the fungal communities associated with each crop are quite different. These results provide a framework for examining microbial components that might contribute to the health of Vaccinium spp. crops in New Jersey and other parts of the northeastern U.S.
Cold tolerance identification of nine Rosa L. materials and expression patterns of genes related to cold tolerance in Rosa hybrida
Authors: Wang Hongli , Cheng Xi , Shi Qiyu , Xu Jie , Chen Dongliang , Luo Chang , Liu Hua , Cao Li , Huang Conglin
Published: June 2023

Members of theRosagenus have a high ornamental value, but their cultivation area is limited by their sensitivity to cold temperatures. The aim of this study was to evaluate the cold tolerance of a range ofRosamaterials, and then determine which genes were related to cold tolerance. NineRosamaterials were subjected to a cold treatment. To identify genes related to cold tolerance,R. hybridawas treated at −15°C for 10 min, and leaves collected before and after this treatment were collected for RNA-Seq analyses. The transcript profiles of four DEGs (POD17, NDUFA9, PMA1, andb-Amy1) inR. hybridawere determined by qRT-PCR at 0 h, 1 h, 2 h, and 3 h at −15°C. NineRosamaterials were subjected to a cold treatment, and the most cold-tolerant materials were identified as those that showed the lowest levels of electrolyte leakage and the best recovery after 30 d of growth. The most cold-tolerant materials wereRosa hybrida, Rosa rugosa‘Pingyin 12’, andRosa rugosa. In total, 204 significantly differentially expressed genes (DEGs) were identified, of which 88 were significantly up-regulated and 116 were significantly down-regulated under cold conditions. Gene Ontology classification and Kyoto Encyclopedia of Genes and Genomes pathway analyses showed that the DEGs were enriched in 57 pathways, especially starch and sucrose metabolism, phenylpropane biosynthesis, MAPK signaling, fructose and mannose metabolism, and oxidative phosphorylation. By transcriptional analysis,PMA1, which was related to H+ATPase activity, was continuously up-regulated, but the transcript levels ofPOD17, NDUFA9, andβ-Amy1fluctuated during the freezing treatment. This research uncovered scarce cold-resistant materials and layed the foundation for further research on the cold tolerance mechanism ofRosaplants and the breeding of cold-tolerant varieties.
Critical action to redress systemic oppression: a person-centered approach
Authors: Vierra Kristin , Barrita Aldo , Wong-Padoongpatt Gloria , Robnett Rachael D.
Published: June 2023

Introduction

In 2020, public outcry against police brutality prompted many social media users to post black squares and use the hashtag #BlackLivesMatter (BLM). Many of the people who posted these squares were engaging in performative action in the sense that they failed to engage with BLM’s history and core principles. Drawing from a critical action framework, the current research seeks to more deeply understand what drives people to engage in more versus less impactful forms of action to resist systemic oppression.

Methods

We employed a mixed-methods and person-centered methodological approach with the goal of providing nuanced information about factors that distinguish among individuals who engage in different forms of action. Participants were 359 undergraduates who reported that they engaged in some form of action to support BLM.

Findings

Latent profile analysis identified three subgroups (i.e., latent classes) in the larger sample, which we labeled (1) intentional action, (2) intermediate action, and (3) passive action. Participants in each latent class differed from one another in their sociopolitical attitudes, sociodemographic background, and level of action to support BLM. Through the qualitative coding process, the research team unearthed three overarching themes and a range of subthemes that help to explain why the members of each class engaged in different forms of action.

Discussion

We conclude by proposing a flexible intervention that may motivate individuals to engage in critical action to support BLM.
Multi-scale convolutional recurrent neural network for psychiatric disorder identification in resting-state EEG
Authors: Yan Weizheng , Yu Linzhen , Liu Dandan , Sui Jing , Calhoun Vince D. , Lin Zheng
Published: June 2023

Background

Accurate classification based on affordable objective neuroimaging biomarkers are important steps toward designing individualized treatment.

Methods

In this work, we investigated a deep learning classification model, multi-scale convolutional recurrent neural network (MCRNN), to explore psychiatric disorder-related biomarkers by leveraging the spatiotemporal information of resting-state EEG (rsEEG) using a multiple psychiatric disorder database containing 327 individuals diagnosed with schizophrenia, bipolar, major depressive disorders, and healthy controls. All subjects were mapped to a shared low-dimensional subspace for intuitively interpreting the inter-relationship and separation of psychiatric disorders.

Results

Psychiatric disorders were identified using rsEEG with high accuracy ranged from 78.6 to 91.3% in patient vs. controls two-class classification, and 68.2% in four-class classification. The control-to-schizophrenia trajectory interpretated by the model was consistent with the disease severity in clinical observation.

Conclusion

The MsRNN demonstrated a capability in extracting discriminative rsEEG biomarkers for psychiatric disorder classification, indicating its potential to facilitate our understanding of psychiatric disorders and monitoring interventions.
Determinants, inequalities, and spatial patterns of diarrhea in the Peruvian under-five population: findings from nationally representative survey data
Authors: Hernández-Vásquez Akram , Vargas-Fernández Rodrigo , Turpo Cayo Efrain Y.
Published: June 2023

Objective

To determine the associated factors, decompose the socioeconomic inequalities, and analyze the spatial distribution of childhood diarrhea in Peru.

Methods

A cross-sectional analytical study was conducted using data from the National Demographic and Family Health Survey 2021. The dependent variable was the presence of diarrhea in the last two weeks. Three types of analysis were performed: (i) to evaluate the associated factors, generalized linear models of the Poisson family with logarithmic link were applied and prevalence ratios with their 95% confidence intervals were reported; (ii) for the analysis of inequalities, a decomposition of the Erreygers concentration index was performed using a generalized linear model; and (ii) a spatial autocorrelation analysis, hot spot analysis and cluster and outlier analysis were performed.

Results

A total of 18,871 children under 5 years of age were included. The prevalence of diarrhea in this population was 10.0%. Determinants such as being aged 0–23 months, being male, belonging to the poorest, poorer and rich wealth index, and residing in the Highlands and Jungle increased the probability of presenting diarrhea. In the decomposition analysis, diarrhea had a pro-poor orientation, with the greatest contributors were age 0–23 months, belonging to the poorest and poorer wealth indexes, and residing in the Highlands and Jungle. Spatial analysis showed that the highest concentrations and occurrence of this event were observed in departments of the Highlands and Jungle.

Conclusion

Government institutions seeking to reduce the numbers and burden of childhood diarrhea should focus their strategies on promoting hygiene measures and improving access to water and sanitation services, especially in poor populations living in the Peruvian Highlands and Jungle.
Integrating maternal, newborn, child health and non-communicable disease care in the sustainable development goal era
Authors: Akselrod Svetlana , Banerjee Anshu , Collins Téa E. , Acharya Shambhu , Artykova Nazira , Askew Ian , Berdzuli Nino , Diorditsa Sergey , Eggers Rudolf , Farrington Jill , Jakab Zsuzsanna , Ferreira-Borges Carina , Mikkelsen Bente , Azzopardi-Muscat Natasha , Olsavszky Victor , Park Kidong , Sobel Howard , Tran Huong , Vujnovic Melita , Weber Martin , Were Wilson , Yaqub Nuhu , Berlina Daria , Dunlop Catherine L. , Allen Luke N.
Published: June 2023

Noncommunicable diseases (NCDs) and maternal newborn and child health (MNCH) are two deeply intertwined health areas that have been artificially separated by global health policies, resource allocations and programming. Optimal MNCH care can provide a unique opportunity to screen for, prevent and manage early signs of NCDs developing in both the woman and the neonate. This paper considers how NCDs, NCD modifiable risk factors, and NCD metabolic risk factors impact MNCH. We argue that integrated management is essential, but this faces challenges that manifest across all levels of domestic health systems. Progress toward Sustainable Development targets requires joined-up action.
Measuring the importance of influencing factor for COVID-19 vaccination intention in China
Authors: Su Yue , Li Sijia , Xue Jia , Li Ang , Zhu Tingshao
Published: June 2023

Background

Vaccination is considered an effective approach to deter the spread of coronavirus disease (COVID-19). However, vaccine hesitancy is a common issue that makes immunization programs more challenging. To promote vaccination in a targeted and efficient way, this study aims to develop and validate a measurement tool for evaluating the importance of influencing factors related to COVID-19 vaccination intention in China, and to examine the demographic differences.

Methods

In study 1, we developed a Factor Importance Evaluation Questionnaire (FIEQ) based on semi-structured interview results and used exploratory factor analysis (EFA) to explore its factor structure. In study 2, we verified the four-factor structure of FIEQ by confirmatory factor analysis (CFA). We then administered FIEQ to Chinese participants and conducted a student t-test and analysis of variance to examine the differences in the importance evaluation of factors based on gender and educational level.

Results

In study 1, we developed a four-factor construct and retained 20 items after EFA (N = 577), with acceptable reliability (alpha = 0.87) and validity. In study 2, we found that the model fit was good (χ2 = 748.03 (162), p < 0.001, GFI = 0.949, RMSEA = 0.049, SRMR = 0.048, AGFI = 0.934), and reliability was acceptable (alpha = 0.730) (N = 1,496). No gender difference was found in factor importance. However, individuals with different educational levels reported significantly different importance evaluations of three factors, including perceived benefits and social norms (F = 3.786, p = 0.005), perceived influences from reference groups (F = 17.449, p < 0.001), and perceived risks (F = 2.508, p = 0.04).

Conclusion

This study developed and validated FIEQ for measuring the importance of influencing factors related to the COVID-19 vaccination intention in Chinese participants. Moreover, our findings suggest that the educational level may play a role in how individuals evaluate the importance of factors. This study provides insights into the concerns that individuals have regarding vaccination and offers potentially effective and targeted strategies for promoting COVID-19 vaccination.
National screening for anxiety and depression in Saudi Arabia 2022
Authors: Alhabeeb Abdulhameed Abdullah , Al-Duraihem Rashed Abdullah , Alasmary Saeed , Alkhamaali Zaied , Althumiri Nora A. , BinDhim Nasser F.
Published: June 2023

Background

Mental health disorders, such as major depressive disorder (MDD) and generalized anxiety disorder (GAD), represent a significant public health concern in Saudi Arabia. This study aims to provide a recent mental health screening prevalence, including anxiety and depression screening in the general public and to explore the associated risk factors.

Methods

A cross-sectional study was conducted, employing a phone interview survey with 6,015 participants, using a quota sampling strategy to ensure equal representation of both sexes and administrative regions. The study assessed the prevalence of MDD and GAD risk and examined demographic, socioeconomic, and lifestyle factors associated with these mental health disorders.

Results

The national prevalence of people at risk of MDD and GAD were found to be 12.7 and 12.4%, respectively. Low diagnosis and treatment rates were observed, with only 1.5 and 0.5% of participants currently diagnosed and treated for depression and anxiety, respectively. Risk factors for MDD and GAD included female sex, lower education and income levels, smoking, and waterpipe use. Protective factors included physical activity, participation in volunteering activities and the practice of daily hobbies in the last 30 days.

Conclusion

The relatively high prevalence of MDD and GAD risk and low diagnosis and treatment rates in Saudi Arabia emphasize the need for increased mental health promotion, early detection, and treatment accessibility. The study highlights the importance of addressing modifiable risk factors and fostering protective factors through targeted interventions. Future research should focus on longitudinal associations, potential mediators and moderators, and the development of culturally appropriate and evidence-based interventions to enhance mental health outcomes in the region.
Virtual reality check: a comparison of virtual reality, screen-based, and real world settings as research methods for HRI
Authors: Plomin Jana , Schweidler Paul , Oehme Astrid
Published: June 2023

To reduce costs and effort, experiments in human-robot interaction can be carried out in Virtual Reality (VR) or in screen-based (SB) formats. However, it is not well examined whether robots are perceived and experienced in the same way in VR and SB as they are in the physical world. This study addresses this topic in a between-subjects experiment, measuring trust and engagement of an interaction with a mobile service robot in a museum scenario. Measures were made in three different settings, either the real world, in VR or in a game-like SB and then compared with an ANOVA. The results indicate, that neither trust nor engagement differ dependent on the experimental setting. The results imply that both VR and SB are eligible ways to explore the interaction with a mobile service robot, if some peculiarities of each medium are taken into account.
Present situation and development prospects of the diagnosis and treatment of rotator cuff tears
Authors: Zhou Tianjun , Han Chang , Weng Xisheng
Published: June 2023

Rotator cuff tears are an important cause of shoulder pain and are caused by degeneration or trauma of the shoulder tendon at the anatomical neck of the humeral head. The understanding and research of rotator cuff tears have a history of hundreds of years, and their etiology, diagnosis, and treatment have a complete system, but some detailed rules of diagnosis and treatment still have room for development. This research paper briefly introduces the diagnosis and treatment of rotator cuff tears. The current situation and its valuable research direction are described.
The bovine nasal fungal community and associations with bovine respiratory disease
Authors: Centeno-Martinez Ruth Eunice , Mohan Suraj , Davidson Josiah Levi , Schoonmaker Jon P. , Ault Aaron , Verma Mohit S. , Johnson Timothy A.
Published: June 2023

Introduction

Effective identification and treatment of bovine respiratory disease (BRD) is an ongoing health and economic issue for the dairy and beef cattle industries. Bacteria pathogens Pasteurellamultocida, Mycoplasmabovis, Mannheimia haemolytica, and Histophilus somni and the virus Bovine herpesvirus-1 (BHV-1), Bovine parainfluenza-3 virus (BPIV-3), Bovine respiratory syncytial virus (BRSV), Bovine adenovirus 3 (BAdV3), bovine coronavirus (BoCV) and Bovine viral diarrhea virus (BVDV) have commonly been identified in BRD cattle; however, no studies have investigated the fungal community and how it may also relate to BRD.

Methods

The objective of this study was to understand if the nasal mycobiome differs between a BRD-affected (n = 56) and visually healthy (n = 73) Holstein steers. Fungal nasal community was determined by using Internal Transcribed Spacer (ITS) sequencing.

Results

The phyla, Ascomycota and Basidiomycota, and the genera, Trichosporon and Issatchenkia, were the most abundant among all animals, regardless of health status. We identified differences between healthy and BRD animals in abundance of Trichosporon and Issatchenkia orientalis at a sub-species level that could be a potential indicator of BRD. No differences were observed in the nasal fungal alpha and beta diversity between BRD and healthy animals. However, the fungal community structure was affected based on season, specifically when comparing samples collected in the summer to the winter season. We then performed a random forest model, based on the fungal community and abundance of the BRD-pathobionts (qPCR data generated from a previous study using the same animals), to classify healthy and BRD animals and determine the agreement with visual diagnosis. Classification of BRD or healthy animals using ITS sequencing was low and agreed with the visual diagnosis with an accuracy of 51.9%. A portion of the ITS-predicted BRD animals were not predicted based on the abundance of BRD pathobionts. Lastly, fungal and bacterial co-occurrence were more common in BRD animals than healthy animals.

Discussion

The results from this novel study provide a baseline understanding of the fungal diversity and composition in the nasal cavity of BRD and healthy animals, upon which future interaction studies, including other nasal microbiome members to further understand and accurately diagnose BRD, can be designed.
Optimum Cultivation Model Increased the Grain Yield of Ratoon Rice and Nitrogen Agronomic Efficiency by Improving Root Morphological Traits and Stubble Character of the Main Rice
Authors: Zheng Huabin , Liu Shanzhen , Zou Dan , He Zaizhou , Chen Yuanwei , Gai Panpan , Wang Weiqin , Tang Qiyuan
Published: June 2023

The present study investigated the effects of different integrated cultivation treatments on the yield, nitrogen agronomic efficiency (AEN), and growth performance of a ratoon rice system. A two-year field experiment was carried out during 2021–2022 using a randomized block design. Cultivation treatments included the farmers’ practice model (control, CK), high-yield and high-efficiency model (T1), enhanced-high-yield and high-efficiency model (T2), and the no-N model (N0). The grain yield, yield components, root traits, and stubble characteristics were determined. The results showed that T2 treatment significantly increased the grain yield and AEN by 53% and 236%, respectively, compared with CK. Similarly, the stubble weight, non-structure carbohydrate contents, and the root attributes were significantly improved under T2 treatment. The correlation analysis suggested that ratoon rice yield and AEN was significantly and positively correlated with root weight (p < 0.01) and the stubble parameters including stubble weight (p < 0.01), starch content (p < 0.01), and soluble sugar content (p < 0.01). Additionally, a significant and positive correlation between root weight and stubble weight (p < 0.05) was determined in our study. Consequently, our work highlights the benefits of integrating planting density, nitrogen/water management, and other practices to achieve high AEN and grain yield of the ratoon rice by improving root trait and stubble characteristics of the main rice.
Comparative Assessment of the Stress Response of Cattle to Common Dairy Management Practices
Authors: Koenneker Katja , Schulze Martin , Pieper Laura , Jung Markus , Schmicke Marion , Beyer Fritz
Published: June 2023

While studies have been conducted examining the stress response of dairy cattle to individual acute and chronic stressors, the results are difficult to compare due to differences in study design and analysis methods. The aim of the present study was to conduct a comparative assessment of the impact of eight common stimuli: artificial insemination (AI), embryo transfer (ET), morning milking (MM), evening milking (EM), veterinary examination (VE), ultrasound examination (US), hoof trimming (HT), and natural breeding (NB) on the hypothalamic-pituitary-adrenal axis and milk production of 24 Holstein-Friesian cattle. After random allocation into control and treatment groups, a total of five blood samples were collected 40 min (Ba1) and 20 min (Ba2) prior to stimulus application, immediately following the stimulus (St), as well as 20 min (Re1) and 40 min (Re2) post-stimulus. A comparison between the overall serum cortisol concentrations in the treatment groups showed a significant difference between HT to AI (p = 0.006), ET (p = 0.010), MM (p = 0.021), VE (p = 0.009), EM (p = 0.007), and US (p = 0.010), except for NB (p = 0.542). There is no significant difference between the control groups (p > 0.05). The stimuli HT (p < 0.001) and NB (p < 0.001) showed significant increases in cortisol following stimulus application, and the levels failed to decrease significantly by sample Re2. No significant differences in daily milk yield (kg) were measured amongst the tested stimuli (p = 0.472) nor amongst the groups ‘Control’, ‘Treatment’ and ‘no stimulus’ (p = 0.350). In conclusion, when factors such as increased physical activity, novel social interaction, sexual arousal, and a more intense restriction of movement are present, the animal’s perceived controllability and predictability might decrease, affecting the animal’s response to stress. Treatments carried out while animals are restrained in a headlock while remaining within their regular group likely result in a less intense activation of the HPA axis.
Defining a “Good Death”: Exploring Veterinarians’ Perceptions of Companion Animal Euthanasia
Authors: Kogan Lori R. , Cooney Kathleen A.
Published: June 2023

This study was designed to determine how veterinarians define a good euthanasia experience. This information is used to generate a working definition of companion animal euthanasia that aligns with animal welfare standards and pet owners’ expectations. An electronic survey distributed via veterinary-related social media (Facebook, Instagram) and listservs were completed by 249 veterinarians who perform feline and/or canine euthanasia. Our results suggest that very few veterinarians feel their veterinary school training adequately prepared them for euthanasia. When veterinarians were asked to rank a list of physiologic conditions and anatomical traits in order of euthanasia-related concerns, respiratory distress was ranked the highest, while the most concerning physical changes were reported to be indications or impressions of seizures or pain. The most commonly reported euthanasia injection technique performed by participants was intravenous administration of pentobarbital sodium (97%), and most veterinarians preferred having owners present (57%) or having no preference (38%) during euthanasia. Results suggest that veterinarians want a pain-free, anxiety-free experience for the patient, appreciate the use of sedatives before euthanasia, and feel that when available and appropriate, home euthanasia offers several benefits. This understanding of the numerous aspects involved in a good euthanasia experience can help inform the creation of an updated definition of companion animal euthanasia that strives to prioritize the welfare of the patient as well as the needs and expectations of the pet owner.
Synopsis of the Species of Coccidians Reported in Marine Fish
Authors: Saraiva Aurélia , Eiras Jorge C. , Cruz Cristina , Xavier Raquel
Published: June 2023

Eimeriid coccidians represent one of the largest groups of parasitic unicellular organisms and comprise many species of veterinary and medical importance. The aim of this work is to provide information, as complete as possible, concerning the oocyst phase of the species of coccidians (Apicomplexa: Eimeriorina) with four sporocysts, which were reported in marine fish. For each species, the accepted scientific name and respective authorities, its synonyms, reported hosts, site of infection (organ), and geographic distribution have been assembled. Available information on morphology (oocyst, sporocyst, and sporozoite) and GenBank accession numbers were also compiled. A total of 100 species of coccidians were described and reported from 60 families of marine fishes. Most species have been described from marine teleosts, with only 14 of the species described from marine elasmobranchs. Most of the species reported in marine fish belong to the genera Eimeria and Goussia, and only a handful belong to the genera Epieimeria, Calyptospora, and Crystallospora. Although marine coccidians have began to be described for more than one century, the collection of genetic data on marine fish coccidians only started in the 2010s and remains largely disconnected from the morphological analysis of specimens, which is available for only six formally described species.
Young Women’s Attitudes and Behaviors in Treatment and Prevention of UTIs: Are Biomedical Students at an Advantage?
Authors: Jerkovic Ivan , Bukic Josipa , Leskur Dario , Seselja Perisin Ana , Rusic Doris , Bozic Josko , Zuvela Tomislav , Vuko Sara , Vukovic Jonatan , Modun Darko
Published: June 2023

We wanted to investigate whether students who study within biomedical fields (i.e., medicine, pharmacy science) differ from those whose studies are not connected to the biomedical field in terms of their attitudes and behaviors related to urinary tract infections (UTIs). This was a cross-sectional survey-based study conducted among 392 female students, of whom 243 attended a biomedical school and 149 (38.0%) attended a non-biomedical school, using a previously published tool. The survey was distributed as an online link via student representatives at different faculties. Only 22 (5.6%) of women felt that they could not recognize a UTI. A greater proportion of biomedical students wiped front to back, while significantly more non-biomedical students chose cotton underwear and avoided daily sanitary pads compared to biomedical students. As many as 215 (54.8%) women stated that they used cranberry preparations. Biomedical students showed greater awareness about possible resistance to repeated treatment (p = 0.002) and greater knowledge of possible interactions of antibiotics (p < 0.001). This study reveals that young women are confident in recognizing an UTIs, are open to alternative treatments, and would consider UTI management in a pharmacy setting. However, it reveals that there might be gaps in their knowledge regarding antibiotic resistance risks, possible interactions, and efficacy of available preparations, as participants from the group of biomedical students showed greater knowledge and different behaviors.
ZIF-8 Nanoparticles Induce Behavior Abnormality and Brain Oxidative Stress in Adult Zebrafish (Danio rerio)
Authors: Jin Liang , Wang Sijing , Chen Chen , Qiu Xuchun , Wang Chong-Chen
Published: June 2023

Zeolitic imidazolate framework-8 nanoparticles (ZIF-8 NPs) are typical metal–organic framework (MOF) materials and have been intensively studied for their potential application in drug delivery and environmental remediation. However, knowledge of their potential risks to health and the environment is still limited. Therefore, this study exposed female and male zebrafish to ZIF-8 NPs (0, 9.0, and 90 mg L−1) for four days. Subsequently, variations in their behavioral traits and brain oxidative stress levels were investigated. The behavioral assay showed that ZIF-8 NPs at 90 mg/L could significantly decrease the locomotor activity (i.e., hypoactivity) of both genders. After a ball falling stimulation, zebrafish exposed to ZIF-8 NPs (9.0 and 90 mg L−1) exhibited more freezing states (i.e., temporary cessations of movement), and males were more sensitive than females. Regardless of gender, ZIF-8 NPs exposure significantly reduced the SOD, CAT, and GST activities in the brain of zebrafish. Correlation analysis revealed that the brain oxidative stress induced by ZIF-8 NPs exposure might play an important role in their behavioral toxicity to zebrafish. These findings highlight the necessity for further assessment of the potential risks of MOF nanoparticles to aquatic species and the environment.
AGS Gastric Cells: Antioxidant Activity and Metabolic Effects of Phenolic Extracts from Different Monocultivar Virgin Olive Oils
Authors: Faraoni Paola , Bellumori Maria , Cecchi Lorenzo , Zonfrillo Beatrice , Innocenti Marzia , Gnerucci Alessio , Mulinacci Nadia , Ranaldi Francesco
Published: June 2023

The effects of the phenolic compounds of extra virgin olive oil (EVOO) on AGS cells have never been studied so far, which is the aim of this study. The profiles of the main phenolic components in EVOOs, mainly secoiridoid compounds derived from the transformation of oleuropein during the olive milling process, were evaluated and compared. Oils of different origins were evaluated aiming at verifying whether chemical differences in the phenolic composition of the dry extracts played a role in the metabolism and in maintaining the cellular redox state of AGS cells. The following key enzymes of some metabolic pathways were studied: lactate dehydrogenase, enolase, pyruvate kinase, glucose 6-phosphate dehydrogenase, citrate synthase, 3-Hydroxyacyl-CoA dehydrogenase and hexokinase. As confirmed through PCA analysis, pretreatments with the dry extracts of EVOOs at different concentrations appeared to be able to counteract the enzymatic activity alterations due to oxidative stress induced by H2O2 1 mM and 2 mM. The studied phytocomplexes showed the ability to protect AGS cells from oxidative damage and the secoiridoid derivatives from both oleuropein and ligstroside contributed to the observed effects. The results suggested that EVOOs with medium to high concentrations of phenols can exert this protection.
Biofilm Formation, and Related Impacts on Healthcare, Food Processing and Packaging, Industrial Manufacturing, Marine Industries, and Sanitation–A Review
Authors: Shineh Ghazal , Mobaraki Mohammadmahdi , Perves Bappy Mohammad Jabed , Mills David K.
Published: June 2023

Biofilm formation can lead to problems in healthcare, water distribution systems, food processing and packaging, industrial manufacturing, marine industries, and sanitation. These microbial communities can proliferate on biotic or abiotic surfaces, and are responsible for human disease and decreasing production efficiency and service equipment life in many industrial fields. The formation of biofilm starts with the attachment of bacteria to the surface, followed by bacterial proliferation and maturation of the microbial community. After forming a biofilm, bacteria not resistant to antimicrobial agents in their planktonic forms can turn resistant. The antibiotic resistance of bacterial biofilm, and the association of biofilms in generating infectious diseases in humans, highlight the need for designing novel and successful antibacterial, anti-biofilm, or anti-infection materials. This paper aims to review the mechanism of biofilm formation, the impact on different industries, the interaction mechanism of nanoparticles with bacteria, and strategies to design anti-biofilm materials. Examples of designing anti-infection bio-implants, coatings, medical devices, wound dressings, and sutures are reviewed.