bluuebunny/crossref_metadata_2025 huggingface dataset:
Optimising Additive Manufacturing to Produce PLA Sandwich Structures by Varying Cell Type and Infill: Effect on Flexural Properties
Authors: Marabello Gabriele , Chairi Mohamed , Di Bella Guido
Published: September 2024

The objective of this research is to optimize additive manufacturing processes, specifically Fused Filament Fabrication (FFF) techniques, to produce sandwich structures. Mono-material specimens made of polylactic acid (PLA) were produced, where both the skin and core were fabricated in a single print. To optimize the process, variations were made in both the base cell geometry of the core (Tri-Hexagon and Gyroid) and the core infill (5%, 25%, 50%, and 75%), evaluating their effects on static three-point bending behavior. Optical microscopy was employed to assess both the structure generated by additive manufacturing and the fracture modes. The findings reveal that increasing the infill, and thus the core density, enhances the mechanical properties of the structure, although the improvement is such that samples with 50% infill already demonstrate excellent performance. The difference between hexagonal and Gyroid structures is not significant. Based on microscopic analyses, it is believed that the evolution of 3D printers, from open to closed chamber designs, could significantly improve the deposition of the various layers.
Ecological-Dynamic Approach vs. Traditional Prescriptive Approach in Improving Technical Skills of Young Soccer Players
Authors: Esposito Giovanni , Ceruso Rosario , Aliberti Sara , Raiola Gaetano
Published: September 2024

Background: This study contributes to expanding the existing literature on learning technical skills in youth soccer by comparing the effectiveness of different training approaches in the development of passing skills. The ecological-dynamic approach, which emphasizes the continuous and adaptive interaction between the athlete and the environment, is analyzed in comparison to the traditional prescriptive approach, which relies on predefined techniques and exercises. The aim of the study is to determine which of the two approaches is more effective in improving the performance of young soccer players. Methods: Thirty players (age 12 ± 1.2 years) were randomly assigned to two groups: the ecological-dynamic group (ECG) and a control group (CON). Both groups underwent an eight-week training program with equal sessions. The ECG group’s training focused on adjusting constraints like the learning environment, game rules, and visual restrictions to boost adaptability and problem-solving skills. The CON group followed a traditional prescriptive approach with specific instructions, goal setting, immediate feedback, and structured exercise progression. Passing abilities were evaluated before and after the program using the Loughborough Soccer Passing Test, with a retention test administered five weeks later. Descriptive statistics, including mean values and percentage improvements, were used. A repeated measures ANOVA compared differences between the groups. Results: The analysis revealed a significant Occasion × Group interaction for all performance variables, indicating that the ECG group experienced greater improvements than the CON group. Specifically, the ECG group showed significant reductions in Trial Time (p = 0.001, ηp2 = 0.6), Penalty Time (p = 0.016, ηp2 = 0.4), and Overall Performance (p = 0.011, ηp2 = 0.8) from pre-test to post-test. However, these improvements did not persist into the retention test (p = 0.131, p = 0.792, and p = 0.192, respectively). The CON group also improved significantly in Trial Time (p = 0.003), Penalty Time (p = 0.002), and Overall Performance (p = 0.001) from pre-test to post-test, but with smaller effect sizes and no sustained gains at retention. Conclusions: The ecological-dynamic approach (EDG) has proven to be more effective in enhancing passing skills compared to the traditional prescriptive approach (CON). Although both methods led to performance improvements, the EDG group achieved more significant progress.
Pros and Cons of (NH4)2S Solution Treatment of p-GaN/Metallization Interface: Perspectives for Laser Diode
Authors: Levchenko Iryna , Kryvyi Serhii , Kamińska Eliana , Smalc-Koziorowska Julita , Grzanka Szymon , Kacperski Jacek , Nowak Grzegorz , Kret Sławomir , Marona Łucja , Perlin Piotr
Published: September 2024

The impact of wet treatment using an (NH4)2S-alcohol solution on the interface state of the p-GaN/Ni/Au/Pt contact system and laser diode processing was investigated. Sulfur wet cleaning resulted in reduced surface roughness and contact resistivity. The lowest specific contact resistance (ρc < 1 × 10−4 Ω·cm2) was achieved with samples treated with an (NH4)2S-isopropanol solution, whereas the highest resistivity (ρc = 3.3 × 10−4 Ω·cm2) and surface roughness (Ra = 16 nm) were observed in samples prepared by standard methods. Annealing the contact system in an N2 + O2 + H2O atmosphere caused degradation through species inter-diffusion and metal-metal solid solution formation, irrespective of the preparation method. Standard prepared substrates developed a thin GaN-Au intermediate layer at the interface after heat treatment. Enhanced adhesion and the absence of GaN decomposition were observed in samples additionally cleaned with the (NH4)2S-solvent solution. Complete oxidation of nickel to NiO was observed in samples that underwent additional sulfur solution treatment. The intensity of metal species mixing and nickel oxidation was influenced by the metal diffusion rate and was affected by the initial state of the GaN substrate obtained through different wet treatment methods.
Fatigue Life Prediction of a SAE Keyhole Specimen as a Subcase of Certification by Analysis
Authors: Wu Xijia , Zhang Zhong , Paraschivoiu Dany
Published: September 2024

To advance the technology of Certification by Analysis (CbA), as called for by the aerospace industry, the fatigue problems of SAE keyhole specimens are analyzed to demonstrate a subcase of CbA. First, phenomena identification and ranking table (PIRT) analysis is performed. Second, modeling of the key phenomena is conducted, and finally, verification and validation with the experimental results are achieved. In particular, the elastic/elastoplastic stress distributions in the keyhole specimens are obtained using the finite element method (FEM). Plasticity correction for stress/strain at the notch root is made using the modified Neuber’s rule along with the Ramberg–Osgood equation. The low cycle fatigue (LCF) crack nucleation life is analytically predicted using the modified Tanaka–Mura model, a.k.a. the TMW model, given the material’s elastic modulus, Poisson’s ratio, Burgers vector, and surface energy, without the need for coupon fatigue data regression. The Tomkins equation is used to simulate plastic crack growth within the notch plastic zone. The above analytical life predictions are validated against the SAE keyhole specimen tests, becoming the first successful case of fatigue CbA at a sub-element level.
Performance of Low-Dimensional Solid Room-Temperature Photodetectors—Critical View
Authors: Rogalski Antoni , Hu Weida , Wang Fang , Martyniuk Piotr
Published: September 2024

In the last twenty years, nanofabrication progress has allowed for the emergence of a new photodetector family, generally called low-dimensional solids (LDSs), among which the most important are two-dimensional (2D) materials, perovskites, and nanowires/quantum dots. They operate in a wide wavelength range from ultraviolet to far-infrared. Current research indicates remarkable advances in increasing the performance of this new generation of photodetectors. The published performance at room temperature is even better than reported for typical photodetectors. Several articles demonstrate detectivity outperforming physical boundaries driven by background radiation and signal fluctuations. This study attempts to explain these peculiarities. In order to achieve this goal, we first clarify the fundamental differences in the photoelectric effects of the new generation of photodetectors compared to the standard designs dominating the commercial market. Photodetectors made of 2D transition metal dichalcogenides (TMDs), quantum dots, topological insulators, and perovskites are mainly considered. Their performance is compared with the fundamental limits estimated by the signal fluctuation limit (in the ultraviolet region) and the background radiation limit (in the infrared region). In the latter case, Law 19 dedicated to HgCdTe photodiodes is used as a standard reference benchmark. The causes for the performance overestimate of the different types of LDS detectors are also explained. Finally, an attempt is made to determine their place in the global market in the long term.
Wear Prediction of Functionally Graded Composites Using Machine Learning
Authors: Fathi Reham , Chen Minghe , Abdallah Mohammed , Saleh Bassiouny
Published: September 2024

This study focuses on the production of functionally graded composites by utilizing magnesium matrix waste chips and cost-effective eggshell reinforcements through centrifugal casting. The wear behavior of the produced samples was thoroughly examined, considering a range of loads (5 N to 35 N), sliding speeds (0.5 m/s to 3.5 m/s), and sliding distances (500 m to 3500 m). The worn surfaces were carefully analyzed to gain insights into the underlying wear mechanisms. The results indicated successful eggshell particle integration in graded levels within the composite, enhancing hardness and wear resistance. In the outer zone, there was a 25.26% increase in hardness over the inner zone due to the particle gradient, with wear resistance improving by 19.8% compared to the inner zone. To predict the wear behavior, four distinct machine learning algorithms were employed, and their performance was compared using a limited dataset obtained from various test operations. The tree-based machine learning model surpassed the deep neural-based models in predicting the wear rate among the developed models. These models provide a fast and effective way to evaluate functionally graded magnesium composites reinforced with eggshell particles for specific applications, potentially decreasing the need for extensive additional tests. Notably, the LightGBM model exhibited the highest accuracy in predicting the testing set across the three zones. Finally, the study findings highlighted the viability of employing magnesium waste chips and eggshell particles in crafting functionally graded composites. This approach not only minimizes environmental impact through material repurposing but also offers a cost-effective means of utilizing these resources in creating functionally graded composites for automotive components that demand varying hardness and wear resistance properties across their surfaces, from outer to inner regions.
Ultrasound-Assisted Preparation of Hyaluronic Acid-Based Nanocapsules with an Oil Core
Authors: Rajtar Natan , Łazarski Grzegorz , Foryś Aleksander , Otulakowski Łukasz , Trzebicka Barbara , Jamróz Dorota , Kepczynski Mariusz
Published: September 2024

Liquid-core nanocapsules (NCs) coated with amphiphilic hyaluronic acid (AmHA) have been proposed for the preparation of drug and food formulations. Herein, we focused on the use of ultrasound techniques to (i) optimize the polysaccharide chain length with respect to the properties of NCs stabilized with AmHAs and (ii) form oil-core nanocapsules with a coating composed of AmHAs. The results indicate that sonication is a convenient and effective method that allows for a controlled reduction in HA molecular weight. The initial (H-HA) and degraded (L-HA) polysaccharides were then reacted with dodecylamine to obtain hydrophobic HA derivatives (HA-C12s). Then, NCs were prepared based on HA-C12s using ultrasound-assisted emulsification of glyceryl triacetate oil. The nanocapsules coated with L-HA-C12 showed greater stability compared to the longer-chain polysaccharide. Molecular dynamics (MD) simulations revealed that HA-C12 readily adsorbs at the water–oil interphase, adopting a more compact conformation compared to that in the aqueous phase. The dodecyl groups are immersed in the oil droplet, while the main polysaccharide chain remaining in the aqueous phase forms hydrogen bonds or water bridges with the polar part of the triglycerides, thus increasing the stability of the NC. Our research underscores the usefulness of ultrasound technology in preparing suitable formulations of bioactive substances.
Utilization of Tunnel Waste Slag for Cement-Stabilized Base Layers in Highway Engineering
Authors: Deng Junshuang , Yao Yongsheng , Huang Chao
Published: September 2024

The rapid expansion of highway infrastructure in the mountainous regions of China has led to a significant increase in tunnel construction, generating substantial amounts of tunnel waste slag. Concurrently, the development of transportation infrastructure has created a critical shortage of natural aggregates, necessitating the exploration of alternative sustainable sources. This study aimed to conduct a comprehensive evaluation of the physical and mechanical properties of tunnel waste slag and explore its potential for utilization in cement-stabilized base courses for highway engineering applications. The uniaxial compressive strength of the parent rock (tunnel waste slag) ranged from 81 MPa to 89 MPa in the desiccated state, indicating its suitability for use as a construction material. This study also determined the maximum dry density (2.432 g/cm3) and optimal moisture content (5.4%) of cement-stabilized mixtures incorporating recycled aggregates derived from tunnel waste slag. The splitting tensile strength of these mixtures at 28 days varied from 0.48 MPa to 0.73 MPa, demonstrating robust mechanical performance. Moreover, the unconfined compressive strength of these mixtures escalated from 7.0 MPa at 7 days to 11.0 MPa at 90 days, signifying a substantial enhancement in strength over time. These results validate the viability of utilizing tunnel waste slag in highway engineering and furnish valuable insights for designers, concrete manufacturers, and construction firms engaged in the development of cement-stabilized aggregate base courses.
Dynamic Deformation in Nuclear Graphite and Underlying Mechanisms
Authors: Thomas Melonie , Oh Hajin , Schoell Ryan , House Stephen , Crespillo Miguel , Hattar Khalid , Windes William , Haque Aman
Published: September 2024

Time-dependent deformation in nuclear graphite is influenced by the creation and migration of radiation-induced defects in the reactor environment. This study investigates the role of pre-existing defects such as point defect clusters and Mrozowski cracks in nuclear graphite IG-110. Separate specimens were irradiated with a 2.8 MeV Au2+ beam with a fluence of 4.38 × 1014 cm−2 and an 8 MeV C2+ beam with a fluence of 1.24 × 1016 cm−2. Microscopic specimens were either mechanically loaded inside a transmission electron microscope (TEM) or subjected to ex situ indentation-based creep loading. In situ TEM tests showed significant plasticity in regions highly localized around the Mrozowski cracks, resembling slip or ripplocation bands. Slip bands were also seen near regions without pre-existing defects but at very high stresses. Ex situ self-ion irradiation embrittled the specimens and decreased the creep displacement and rate, while heavy ion irradiation resulted in the opposite behavior. We hypothesize that the large-sized gold ions (compared to the carbon atoms) induced interplanar swelling as well as cross-plane channels for increased defect mobility. These findings illustrate the role of pre-existing defects in the dynamic relaxation of stresses during irradiation and the need for more studies into the radiation environment’s impact on the mechanical response of nuclear graphite.
Corrosion Behaviour of Weld Metal of Ultra-High-Strength Steel Weldments in a Sodium Chloride Aqueous Solution
Authors: Ilieva Mariana , Gospodinov Danail , Ferdinandov Nikolay , Radev Rossen
Published: September 2024

As high-strength and ultra-high-strength steels are widely used in all kinds of modern welded constructions, a lot of research is carried out to investigate the mechanical properties of the weldments of these steels, but there is little information on such important characteristics as their corrosion behaviour. This research focuses on the corrosion behaviour of the weld metal of the weldments of S906QL and S700MC steels. The weld metal was tested electrochemically in a 3.5% NaCl aqueous solution via a potentiodynamic scan to determine the corrosion rate and its dependence on the welding gap. No influence of the welding gap on the corrosion rate was found, but the experimental results suggested that the corrosion rate depended on the chemical composition of the filler material and the microstructure of the weld metal.
Evaluation Methods and Coupled Optimization at Macro- and Micro-Scales for Profiled Ring Rolling of Inconel718 Alloy
Authors: Zhu Xinglin , Dong Erting , Qiao Xiaomin , Liu Dong
Published: September 2024

The forming quality of profiled ring rolling not only encompasses macroscopic accuracy but also emphasizes the microstructure. Due to the multiple process parameters and complex metal flow during profiled ring rolling, the various forming defects are difficult to control and difficult to study theoretically. The objective of this study is to establish a comprehensive method for evaluating the forming quality of profiled rings, which considers both the macroscopic forming accuracy and the microstructure. Firstly, the synthetic size factor was defined, and the evolutionary relation between the section forming rate and the diameter growth rate of E-section ring rolling was analyzed in detail. The synthetic size factor can be used to describe the dimensional evolution and evaluate the forming accuracy of the profiled ring rolling process. Taking into full consideration the features of intermittent deformation in local areas, a microstructure evolution model of the Inconel718 alloy during E-section ring rolling, which can accurately predict the recrystallization volume fraction and average grain size of the final ring, was established. Then, combined with finite element simulation, the influence of the rotation speed of the driving roll on the macro-size evolution and microstructure was systematically analyzed. The results indicate that there is often a discrepancy between dimensional accuracy and microstructure uniformity in the optimization trend. For instance, the higher the rotation speed of the driving roll is, the more uniform the microstructure is, but the more difficult it is for the section profile to form. Finally, combined with response surface methodology (RSM), multi-parameter optimization was carried out with section forming accuracy and grain uniformity as the optimization objectives. By using the optimal parameters, an E-section ring with a complete profile and a uniform microstructure was obtained, with a maximum prediction error of the recrystallization volume fraction lower than 5%. The results show that the macroscopic and microscopic quality evaluation methods proposed in this study, as well as the optimization method combining RSM, can be effectively applied to the process optimization of profiled ring rolling.
Genetic Algorithm-Based Data-Driven Process Selection System for Additive Manufacturing in Industry 4.0
Authors: Aljabali Bader Alwomi , Shelton Joseph , Desai Salil
Published: September 2024

Additive manufacturing (AM) has impacted the manufacturing of complex three-dimensional objects in multiple materials for a wide array of applications. However, additive manufacturing, as an upcoming field, lacks automated and specific design rules for different AM processes. Moreover, the selection of specific AM processes for different geometries requires expert knowledge, which is difficult to replicate. An automated and data-driven system is needed that can capture the AM expert knowledge base and apply it to 3D-printed parts to avoid manufacturability issues. This research aims to develop a data-driven system for AM process selection within the design for additive manufacturing (DFAM) framework for Industry 4.0. A Genetic and Evolutionary Feature Weighting technique was optimized using 3D CAD data as an input to identify the optimal AM technique based on several requirements and constraints. A two-stage model was developed wherein the stage 1 model displayed average accuracies of 70% and the stage 2 model showed higher average accuracies of up to 97.33% based on quantitative feature labeling and augmentation of the datasets. The steady-state genetic algorithm (SSGA) was determined to be the most effective algorithm after benchmarking against estimation of distribution algorithm (EDA) and particle swarm optimization (PSO) algorithms, respectively. The output of this system leads to the identification of optimal AM processes for manufacturing 3D objects. This paper presents an automated design for an additive manufacturing system that is accurate and can be extended to other 3D-printing processes.
Solvability of a Class of Fractional Advection–Dispersion Coupled Systems
Authors: Qiao Yan , Lu Tao
Published: September 2024

The purpose of this study is to provide some criteria for the existence and multiplicity of solutions for a class of fractional advection–dispersion coupled systems with nonlinear Sturm–Liouville conditions and instantaneous and non-instantaneous impulses. Specifically, the existence is derived through the Nehari manifold method, and the proof of multiplicity is based on Bonanno and Bisci’s critical point theorem, which does not require proof that the functional satisfies the Palais–Smale condition. Finally, to illustrate the obtained results, an example is provided.
In Vitro In Silico Screening Strategy and Mechanism of Novel Tyrosinase Inhibitory Peptides from Nacre of Hyriopsis cumingii
Authors: Lin Haisheng , Li Fei , Kang Jiaao , Xie Shaohe , Qin Xiaoming , Gao Jialong , Chen Zhongqin , Cao Wenhong , Zheng Huina , Song Wenkui
Published: September 2024

For thousands of years, pearl and nacre powders have been important traditional Chinese medicines known for their skin whitening effects. To prepare the enzymatic hydrolysates of Hyriopsis cumingii nacre powder (NP-HCH), complex enzymatic hydrolysis by pineapple protease and of neutral protease was carried out after the powder was pre-treated with a high-temperature and high-pressure method. The peptides were identified using LC-MS/MS and picked out through molecular docking and molecular dynamics simulations. Subsequently, the tyrosinase inhibitory and antioxidant properties of novel tyrosinase inhibitory peptides were investigated in vitro. In addition, the enzymatic activity of tyrosinase in B16F10 cells as well as melanin content and antioxidant enzyme levels were also examined. The results showed that a tyosinase inhibitory peptide (Tyr-Pro-Asn-Pro-Tyr, YPNPY) with an efficient IC50 value of 0.545 ± 0.028 mM was identified. The in vitro interaction results showed that YPNPY is a reversible competitive inhibitor of tyrosinase, suggesting that it binds to the free enzyme. The B16F10 cell whitening test revealed that YPNPY can reduce the melanin content of B16F10 cells by directly inhibiting the activity of intracellular tyrosinase. Additionally, it indirectly affects melanin production by acting as an antioxidant. These results suggest that YPNPY could be widely used as a tyrosinase inhibitor in whitening foods and drugs.
Morphological, Toxicological, and Biochemical Characterization of Two Species of Gambierdiscus from Bahía de La Paz, Gulf of California
Authors: Fernández-Herrera Leyberth José , Núñez-Vázquez Erick Julián , Hernández-Sandoval Francisco E. , Ceseña-Ojeda Daniel Octavio , García-Davis Sara , Teles Andressa , Virgen-Félix Marte , Tovar-Ramírez Dariel
Published: September 2024

We describe five new isolates of two Gambierdiscus species from Bahía de La Paz in the southern Gulf of California. Batch cultures of Gambierdiscus were established for morphological characterization using light microscopy (LM) and scanning electron microscopy (SEM). Pigment and amino acid profiles were also analyzed using high-performance liquid chromatography (HPLC-UV and HPLC-DAD). Finally, toxicity (CTX-like and MTX-like activity) was evaluated using the Artemia salina assay (ARTOX), mouse assay (MBA), marine fish assay (MFA), and fluorescent receptor binding assay (fRBA). These strains were identified as Gambierdiscus cf. caribaeus and Gambierdiscus cf. carpenteri. Toxicity for CTX-like and MTX-like activity was confirmed in all evaluated clones. Seven pigments were detected, with chlorophyll a, pyridine, Chl2, and diadinoxanthin being particularly noteworthy. For the first time, a screening of the amino acid profile of Gambierdiscus from the Pacific Ocean was conducted, which showed 14 amino acids for all strains except histidine, which was only present in G. cf. caribeaus. We report the presence of Gambierdiscus and Fukuyoa species in the Mexican Pacific, where ciguatera fish poisoning (CFP) cases have occurred.
Prevalence, Management, and Outcomes of Atrial Fibrillation in Paediatric Patients: Insights from a Tertiary Cardiology Centre
Authors: Constante Andreia Duarte , Suarez Joana , Lourenço Guilherme , Portugal Guilherme , Cunha Pedro Silva , Oliveira Mário Martins , Trigo Conceição , Pinto Fátima F. , Laranjo Sérgio
Published: September 2024

Background and Objectives: Atrial fibrillation (AF) is increasingly recognised in paediatric patients, presenting unique challenges in management due to its association with various underlying heart conditions. This study aimed to evaluate the prevalence, management strategies, and outcomes of AF in this population. Materials and Methods: A retrospective analysis was conducted at a tertiary paediatric cardiology centre, including patients aged ≤18 years diagnosed with AF between January 2015 and December 2023. The study focused on demographic details, clinical presentations, treatments, and outcomes. Descriptive statistics were employed to assess treatment efficacy, recurrence rates, and complications. Results: The study included 36 paediatric patients (median age: 15 years, IQR: 13–17; 58% male). Of these, 52.8% had acquired heart disease, 16.7% had congenital heart anomalies, and 16.7% presented with lone AF. The initial management strategies involved electrical cardioversion in 53.3% of patients and pharmacological conversion with amiodarone in 46.7%. Rhythm control therapy was administered to over 80% of the cohort, and 63.9% were placed on oral anticoagulation, predominantly for rheumatic and congenital heart diseases. The overall success rate of rhythm control was 96.2%, with an AF recurrence rate of 3.8%. Ischemic stroke was the most common complication, occurring in three patients, all with underlying rheumatic heart disease. Conclusions: AF in paediatric patients is predominantly associated with rheumatic and congenital heart diseases, though a significant proportion of patients present with lone AF. Despite effective rhythm control in most cases, neurological complications, particularly ischemic strokes in patients with underlying heart disease, remain a critical concern. These findings underscore the need for more comprehensive studies to better understand the aetiology, risk factors, and optimal management strategies for paediatric AF.
Glenohumeral Joint Volume Measurement in Patients with Shoulder Instability: A 3D Volumetric Magnetic Resonance Arthrographic Study
Authors: Güçlü Derya , Ünlü Elif Nisa , Arıcan Mehmet , Acar Oğuzhan , Uludağ Veysel , Oğul Hayri
Published: September 2024

Background and Objectives: This study aimed to compare capsular volume in patients with shoulder instability to that in control subjects without instability using magnetic resonance (MR) arthrography. The objective was to develop a reliable screening method with which to assess shoulder volume. Materials and Methods: In 21 patients with atraumatic shoulder instability and 21 controls, thin-slice 3D volumetric MR arthrography sequences were obtained. MR arthrography images were uploaded to 3D reconstruction, and 3D images were generated. From the 3D reconstructed images, volumetric measurements of rotator interval (RI), anterior and posterior capsular (AC, PC) recesses, biceps tendon sheath (BS), axillary recess (AR), and total glenohumeral joint (TGJ) were performed. Individuals with any extra-articular contrast leakage were also recorded. Results: A retrospective study analyzed a patient group of 21 individuals with shoulder instability (mean age 29.52 ± 12.83 years) and a control group of 21 individuals without instability (mean age 35.71 ± 12.77 years). No statistically significant differences were identified between the groups with regard to age, gender, or side distribution. The mean total joint volume was significantly higher in the instability group (29.85 ± 6.40 cm3) compared to the control group (23.15 ± 3.48 cm3, p = 0.0001). Additionally, the mean volumes of the RI, AC, PC, BS, and AR were all significantly greater in the patient group compared to the control group. Conclusions: 3D volumetric MR arthrographic measurements of the shoulder joint capacity can provide valuable insights for clinical follow-up and guide surgical treatment decisions in cases of atraumatic shoulder instability.
Combined Metabolome and Transcriptome Analyses of Maize Leaves Reveal Global Effect of Biochar on Mechanisms Involved in Anti-Herbivory to Spodoptera frugiperda
Authors: He Tianjun , Chen Lin , Wu Yingjun , Wang Jinchao , Wu Quancong , Sun Jiahao , Ding Chaohong , Zhou Tianxing , Chen Limin , Jin Aiwu , Li Yang , Zhu Qianggen
Published: September 2024

Fall armyworm (FAW, Spodoptera frugiperda) has now spread to more than 26 Chinese provinces. The government is working with farmers and researchers to find ways to prevent and control this pest. The use of biochar is one of the economic and environmentally friendly strategies to increase plant growth and improve pest resistance. We tested four v/v combinations of bamboo charcoal with coconut bran [BC1 (10:1), BC2(30:1), BC3(50:1)] against a control (CK) in maize. We found that plant height, stem thickness, fresh weight and chlorophyll content were significantly higher in BC2, in addition to the lowest FAW survival %. We then compared the metabolome and transcriptome profiles of BC2 and CK maize plants under FAW herbivory. Our results show that the levels of flavonoids, amino acids and derivatives, nucleotides and derivatives and most phenolic acids decreased, while terpenoids, organic acids, lipids and defense-related hormones increased in BC-grown maize leaves. Transcriptome sequencing revealed consistent expression profiles of genes enriched in these pathways. We also observed the increased expression of genes related to abscisic acid, jasmonic acid, auxin and MAPK signaling. Based on these observations, we discussed the possible pathways involved in maize against FAW herbivory. We conclude that bamboo charcoal induces anti-herbivory responses in maize leaves.
Profiling Metabolites with Antifungal Activities from Endophytic Plant-Beneficial Strains of Pseudomonas chlororaphis Isolated from Chamaecytisus albus (Hack.) Rothm.
Authors: Sokołowski Wojciech , Marek-Kozaczuk Monika , Sosnowski Piotr , Sajnaga Ewa , Jach Monika Elżbieta , Karaś Magdalena Anna
Published: September 2024

Fungal phytopathogens represent a large and economically significant challenge to food production worldwide. Thus, the application of biocontrol agents can be an alternative. In the present study, we carried out biological, metabolomic, and genetic analyses of three endophytic isolates from nodules of Chamaecytisus albus, classified as Pseudomonas chlororaphis acting as antifungal agents. The efficiency of production of their diffusible and volatile antifungal compounds (VOCs) was verified in antagonistic assays with the use of soil-borne phytopathogens: B. cinerea, F. oxysporum, and S. sclerotiorum. Diffusible metabolites were identified using chromatographic and spectrometric analyses (HPTLC, GC-MS, and LC-MS/MS). The phzF, phzO, and prnC genes in the genomes of bacterial strains were confirmed by PCR. In turn, the plant growth promotion (PGP) properties (production of HCN, auxins, siderophores, and hydrolytic enzymes, phosphate solubilization) of pseudomonads were bioassayed. The data analysis showed that all tested strains have broad-range antifungal activity with varying degrees of antagonism. The most abundant bioactive compounds were phenazine derivatives: phenazine-1-carboxylic acid (PCA), 2-hydroxy-phenazine, and diketopiperazine derivatives as well as ortho-dialkyl-aromatic acids, pyrrolnitrin, siderophores, and HCN. The results indicate that the tested P. chlororaphis isolates exhibit characteristics of biocontrol organisms; therefore, they have potential to be used in sustainable agriculture and as commercial postharvest fungicides to be used in fruits and vegetables.
Analysis and Formation of Polycyclic Aromatic Hydrocarbons in Canned Minced Chicken and Pork during Processing
Authors: Inbaraj Baskaran Stephen , Lai Yu-Wen , Chen Bing-Huei
Published: September 2024

Polycyclic aromatic hydrocarbons (PAHs) represent important toxic compounds formed in meat products during processing. This study aims to analyze 22 PAHs by QuEChERS coupled with GC–MS/MS in canned minced chicken and pork during processing. After marinating raw minced chicken and pork separately with a standard flavoring formula used for canning meat in Taiwan, they were subjected to different processing conditions including stir-frying, degassing and sterilizing at 115 °C/60 min (low-temperature–long-time, LTLT) and 125 °C/25 min (high-temperature–short-time, HTST). The quantitation of PAHs in these meat products revealed the formation of only three PAHs including acenaphthylene (AcPy), acenaphthene (AcP) and pyrene (Pyr) in canned minced chicken and pork during processing with no significant difference in total PAHs between the meat types. Analysis of PAH precursors showed the presence of benzaldehyde at the highest level, followed by 2-cyclohexene-1-one and trans,trans-2,4-decadienal in canned minced chicken and pork, suggesting PAH formation through the reaction of benzaldehyde with linoleic acid degradation products and of 2-cyclohexene-1-one with C4 compounds through the Diels–Alder reaction, as well as the reaction of trans,trans-2,4-decadienal with 2-butene. Monounsaturated and polyunsaturated fatty acids were present in the largest proportion in LTLT-sterilized chicken/pork, followed by HTST-sterilized chicken/pork and raw chicken/pork, and their levels did not show a high impact on PAH formation, probably due to an insufficient heating temperature and length of time. A two-factorial analysis suggested that PAH formation was not significantly affected by the sterilization condition or meat type. Principal component analysis corroborated the observed results implying the formation of PAHs in canned minced chicken/pork under different processing conditions with an insignificant difference (p > 0.05) between them, with the individual PAH content following the order of Pyr > AcPy > AcP.
Squirting Cucumber, Ecballium elaterium (L.) A. Ritch: An Update of Its Chemical and Pharmacological Profile
Authors: Anzano Attilio , Falco Bruna de , Grauso Laura , Lanzotti Virginia
Published: September 2024

Ecballium elaterium, also known as squirting cucumber, is a plant which is widespread in temperate regions of Europe, Africa and Asia. The plant is considered to be one of the oldest used drugs. In the last decades, E. elaterium has been widely studied as a source of triterpene metabolites named cucurbitacins, often found as glycosylated derivatives, used by the plant as defensive agents. Such metabolites exhibit several biological activities, including cytotoxic, anti-inflammatory, and anti-cancer. Interestingly, the bioactive properties of E. elaterium extracts have been investigated in dozens of studies, especially by testing the apolar fractions, including the essential oils, extracted from leaves and fruits. The purpose of this review is to provide an overview of the chemical profile of different parts of the plants (leaves, flowers, and seeds) analyzing the methods used for structure elucidation and identification of single metabolites. The pharmacological studies on the isolated compounds are also reported, to highlight their potential as good candidates for drug discovery.
Reliability-Based Design Optimization for Polymer Electrolyte Membrane Fuel Cells: Tackling Dimensional Uncertainties in Manufacturing and Their Effects on Costs of Cathode Gas Diffusion Layer and Bipolar Plates
Authors: Vaz Neil , Lim Kisung , Choi Jaeyoo , Ju Hyunchul
Published: September 2024

Polymer Electrolyte Membrane Fuel Cells (PEMFCs) have emerged as a pivotal technology in the automotive industry, significantly contributing to the reduction of greenhouse gas emissions. However, the high material costs of the gas diffusion layer (GDL) and bipolar plate (BP) create a barrier for large scale commercial application. This study aims to address this challenge by optimizing the material and design of the cathode, GDL and BP. While deterministic design optimization (DDO) methods have been extensively studied, they often fall short when manufacturing uncertainties are introduced. This issue is addressed by introducing reliability-based design optimization (RBDO) to optimize four key PEMFC design variables, i.e., gas diffusion layer thickness, channel depth, channel width and land width. The objective is to maximize cell voltage considering the material cost of the cathode gas diffusion layer and cathode bipolar plate as reliability constraints. The results of the DDO show an increment in cell voltage of 31 mV, with a reliability of around 50% in material cost for both the cathode GDL and cathode BP. In contrast, the RBDO method provides a reliability of 95% for both components. Additionally, under a high level of uncertainty, the RBDO approach reduces the material cost of the cathode GDL by up to 12.25 $/stack, while the material cost for the cathode BP increases by up to 11.18 $/stack Under lower levels of manufacturing uncertainties, the RBDO method predicts a reduction in the material cost of the cathode GDL by up to 4.09 $/stack, with an increase in the material cost for the cathode BP by up to 6.71 $/stack, while maintaining a reliability of 95% for both components. These results demonstrate the effectiveness of the RBDO approach in achieving a reliable design under varying levels of manufacturing uncertainties.
Sustainable Assessment of Bio-Colorant from Bakain Bark (Melia azedarach L.) for Dyeing of Cellulosic and Proteinous Fabric
Authors: Batool Fatima , Fatima Maheen , Adeel Shahid , Ahmad Sheikh Asrar , Repon Md. Reazuddin , Haji Aminoddin
Published: September 2024

The current study proceeded to reduce the environmental hazards spreading worldwide due to synthetic dyes. To overcome these problems, eco-friendly natural dyes are introduced as alternative sources of synthetic dyes. The present study was focused on exploring the bio-colorant of the aqueous and acidic extract of the bark of Melia azedarach L. for the dyeing of both silk and cotton samples. The results of the extraction medium specified that the aqueous extract gave maximum colorant solubility and upon fabric dyeing produced higher color strength in contrast to the acidic medium. The optimization experimentation data showed that excellent color strength of silk fabric was found at 45 min dyeing time duration, in 35:1 mL dye extract, and using 2% salt (NaCl) as an exhausting agent, whereas cotton fabric showed the maximum K/S value at 60 min dyeing time, in a 45:1 mL liquor ratio, and with the use of 2% salt. Bio-mordants produce different shades on both fabrics. Bio-mordanting experiments on silk revealed that pre-mordanting with 2% turmeric and 3% pomegranate, and post-mordanting using 3% turmeric and 2% pomegranate produced a darker shade. In the case of cotton, the pre-mordanted samples with 2% turmeric and 3% pomegranate and the post-mordanted samples with 4% turmeric and 4% pomegranate gave the highest color strengths. All the mordanted samples gave excellent fastness ratings. Overall, it has been found that Bakain bark proved to be an excellent source of tannin. The result of this study showed that it could be a cost-effective and eco-friendly dye source for textile progress.
Increasing Fertilization Efficiency of Biomass Ash by the Synergistically Acting Digestate and Extract from Water Plants Sequestering CO2 in Sorghum Crops
Authors: Romanowska-Duda Zdzisława , Janas Regina , Grzesik Mieczysław
Published: September 2024

The utilization of biomass ash in sustainable agriculture and increasing its fertilizing efficiency by biological agents, potentially sequestering CO2, have become important issues for the global economy. The aim of this paper was to investigate the effects of ash from sorghum (Sorghum bicolor L. Moench) and Jerusalem artichoke (Helianthus tuberosus L.) biomass, a biogas plant digestate, and a Spirodela polyrhiza extract, acting alone or synergistically, on soil fertility and the development, health and physiological properties of sorghum plants. The results show novel information concerning differences in the composition and impact of ash, depending on its origin, soil properties and sorghum plant development. Sorghum ash was more effective than that from Jerusalem artichoke. Ash used alone and preferably acting synergistically with the digestate and Spirodela polyrhiza extract greatly increased soil fertility and the growth, biomass yield and health of sorghum plants. These improvements were associated with an increased chlorophyll content in leaves, better gas exchange (photosynthesis, transpiration, stomatal conductance), greater enzyme activity (acid and alkaline phosphatase, RNase, and total dehydrogenase), and a higher biomass energy value. The developed treatments improved environmental conditions by replacing synthetic fertilizers, increasing the sequestration of CO2, solving the ash storage problem, reducing the need for pesticides, and enabling a closed circulation of nutrients between plant and soil, maintaining high soil fertility.
Research Progress in Microporous Materials for Selective Adsorption and Separation of Methane from Low-Grade Gas
Authors: Su Dongrui , Chen Panpan , Li Cunlei , Yan Yongfei , Zhao Ranlei , Yue Qingyou , Qiao Yupeng
Published: September 2024

Given that methane (CH4) and nitrogen (N2) have similar properties, achieving high-purity enrichment of CH4 from nitrogen-rich low-grade gas is extremely challenging and is of great significance for sustainable development in energy and the environment. This paper reviews the research progress on carbon-based materials, zeolites, and MOFs as adsorbent materials for CH4/N2 separation. It focuses on the relationship between the composition, pore size, surface chemistry of the adsorbents, CH4/N2 selectivity, and CH4 adsorption capacity. The paper also highlights that controlling pore size and atomic-scale composition and optimizing these features for the best match are key directions for the development of new adsorbents. Additionally, it points out that MOFs, which combine the advantages of carbon-based adsorbents and zeolites, are likely to become the most promising adsorbent materials for efficient CH4/N2 separation.
Targeted Drug Delivery Strategies for the Treatment of Hepatocellular Carcinoma
Authors: Liu Yonghui , Wu Yanan , Li Zijian , Wan Dong , Pan Jie
Published: September 2024

Hepatocellular carcinoma (HCC) ranks among the most prevalent malignant tumors, exhibiting a high incidence rate that presents a substantial threat to human health. The use of sorafenib and lenvatinib, commonly employed as single-agent targeted inhibitors, complicates the treatment process due to the absence of definitive targeting. Nevertheless, the advent of nanotechnology has injected new optimism into the domain of liver cancer therapy. Nanocarriers equipped with active targeting or passive targeting mechanisms have demonstrated the capability to deliver drugs to tumor cells with high efficiency. This approach not only facilitates precise delivery to the affected site but also enables targeted drug release, thereby enhancing therapeutic efficacy. As medical technology progresses, there is an increasing call for innovative treatment modalities, including novel chemotherapeutic agents, gene therapy, phototherapy, immunotherapy, and combinatorial treatments for HCC. These emerging therapies are anticipated to yield improved clinical outcomes for patients, while minimizing systemic toxicity and adverse effects. Consequently, the application of nanotechnology is poised to significantly improve HCC treatment. This review focused on targeted strategies for HCC and the application of nanotechnology in this area.
Practical Guidelines by the Andalusian Group for Nutrition Reflection and Investigation (GARIN) on Nutritional Management of Patients with Chronic Obstructive Pulmonary Disease: A Review
Authors: Justel Enríquez Alicia , Rabat-Restrepo Juana M. , Vilchez-López Francisco J. , Tenorio-Jiménez Carmen , García-Almeida José M. , Irles Rocamora José-Antonio , Pereira-Cunill José L. , Martínez Ramírez María J. , Molina-Puerta María J. , Molina Soria Juan B. , Rebollo-Pérez María I. , Olveira Gabriel , García-Luna Pedro P.
Published: September 2024

Malnutrition is common in chronic obstructive pulmonary disease (COPD) patients and is associated with worse lung function and greater severity. This review by the Andalusian Group for Nutrition Reflection and Investigation (GARIN) addresses the nutritional management of adult COPD patients, focusing on Morphofunctional Nutritional Assessment and intervention in clinical practice. A systematic literature search was performed using the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) methodology, followed by critical appraisal based on Scottish Intercollegiate Guidelines Network (SIGN) guidelines. Recommendations were graded according to the European Society for Clinical Nutrition and Metabolism (ESPEN) system. The results were discussed among GARIN members, with consensus determined using a Likert scale. A total of 24 recommendations were made: 2(A), 6(B), 2(O), and 14(GPP). Consensus exceeded 90% for 17 recommendations and was 75–90% for 7. The care of COPD patients is approached from a nutritional perspective, emphasizing nutritional screening, morphofunctional assessment, and food intake in early disease stages. Nutritional interventions include dietary advice, recommendations on food group intake, and the impact of specialized nutritional treatment, particularly oral nutritional supplements. Other critical aspects, such as physical activity and quality of life, are also analyzed. These recommendations provide practical guidance for managing COPD patients nutritionally in clinical practice.