bluuebunny/crossref_metadata_2025 huggingface dataset:
Design, Synthesis, and Development of pyrazolo[1,5-a]pyrimidine Derivatives as a Novel Series of Selective PI3Kδ Inhibitors: Part I—Indole Derivatives
Authors: Stypik Mariola , Zagozda Marcin , Michałek Stanisław , Dymek Barbara , Zdżalik-Bielecka Daria , Dziachan Maciej , Orłowska Nina , Gunerka Paweł , Turowski Paweł , Hucz-Kalitowska Joanna , Stańczak Aleksandra , Stańczak Paulina , Mulewski Krzysztof , Smuga Damian , Stefaniak Filip , Gurba-Bryśkiewicz Lidia , Leniak Arkadiusz , Ochal Zbigniew , Mach Mateusz , Dzwonek Karolina , Lamparska-Przybysz Monika , Dubiel Krzysztof , Wieczorek Maciej
Published: August 2022

Phosphoinositide 3-kinase δ (PI3Kδ), a member of the class I PI3K family, is an essential signaling biomolecule that regulates the differentiation, proliferation, migration, and survival of immune cells. The overactivity of this protein causes cellular dysfunctions in many human disorders, for example, inflammatory and autoimmune diseases, including asthma or chronic obstructive pulmonary disease (COPD). In this work, we designed and synthesized a new library of small-molecule inhibitors based on indol-4-yl-pyrazolo[1,5-a]pyrimidine with IC50 values in the low nanomolar range and high selectivity against the PI3Kδ isoform. CPL302253 (54), the most potent compound of all the structures obtained, with IC50 = 2.8 nM, is a potential future candidate for clinical development as an inhaled drug to prevent asthma.
Investigation into the Antihypertensive Effects of Diosmetin and Its Underlying Vascular Mechanisms Using Rat Model
Authors: Ahmad Taseer , Javed Adil , Khan Taous , Althobaiti Yusuf S. , Ullah Aman , Almutairi Farooq M. , Shah Abdul Jabbar
Published: August 2022

Objective: Diosmetin is a flavonoid that is found in many important medicinal plants that have antihypertensive therapeutic potential. Diosmetin has been shown to have antiplatelet, anti-inflammatory and antioxidant properties, which suggests that it could be a potential candidate for use in antihypertensive therapy. Methods: In vivo and in vitro methods were used for our investigation into the antihypertensive effects of diosmetin. Results: Diosmetin significantly decreased the mean arterial pressure (MAP). The effects of diosmetin on the MAP and heart rate were more pronounced in hypertensive rats. To explore the involvement of the muscarinic receptors-linked NO pathway, Nω-nitro-L-arginine methyl ester (L-NAME) and atropine were pre-administered in vivo. The pretreatment with L-NAME did not significantly change the effects of diosmetin on the MAP by excluding the involvement of NO. Unlike L-NAME, the atropine pretreatment reduced the effects of diosmetin on the MAP, which demonstrated the role of the muscarinic receptors. In the in vitro study, diosmetin at lower concentrations produced endothelium-dependent and -independent (at higher concentrations) vasorelaxation, which was attenuated significantly by the presence of atropine and indomethacin but not L-NAME. Diosmetin was also tested for high K+-induced contractions. Diosmetin induced significant relaxation (similar to verapamil), which indicated its Ca2+ antagonistic effects. This was further confirmed by diosmetin shifting the CaCl2 CRCs toward the right due to its suppression of the maximum response. Diosmetin also suppressed phenylephrine peak formation, which indicated its antagonist effects on the release of Ca2+. Moreover, BaCl2 significantly inhibited the effects of diosmetin, followed by 4-AP and TEA, which suggested that the K+ channels had a role as well. Conclusions: The obtained data showed the Ca2+ channel antagonism, potassium channel activation and antimuscarinic receptor-linked vasodilatory effects of diosmetin, which demonstrated its antihypertensive potential.
Application of Mesoporous Silica Nanoparticles in Cancer Therapy and Delivery of Repurposed Anthelmintics for Cancer Therapy
Authors: Koohi Moftakhari Esfahani Maedeh , Alavi Seyed Ebrahim , Cabot Peter J. , Islam Nazrul , Izake Emad L.
Published: August 2022

This review focuses on the biomedical application of mesoporous silica nanoparticles (MSNs), mainly focusing on the therapeutic application of MSNs for cancer treatment and specifically on overcoming the challenges of currently available anthelmintics (e.g., low water solubility) as repurposed drugs for cancer treatment. MSNs, due to their promising features, such as tunable pore size and volume, ability to control the drug release, and ability to convert the crystalline state of drugs to an amorphous state, are appropriate carriers for drug delivery with the improved solubility of hydrophobic drugs. The biomedical applications of MSNs can be further improved by the development of MSN-based multimodal anticancer therapeutics (e.g., photosensitizer-, photothermal-, and chemotherapeutics-modified MSNs) and chemical modifications, such as poly ethyleneglycol (PEG)ylation. In this review, various applications of MSNs (photodynamic and sonodynamic therapies, chemotherapy, radiation therapy, gene therapy, immunotherapy) and, in particular, as the carrier of anthelmintics for cancer therapy have been discussed. Additionally, the issues related to the safety of these nanoparticles have been deeply discussed. According to the findings of this literature review, the applications of MSN nanosystems for cancer therapy are a promising approach to improving the efficacy of the diagnostic and chemotherapeutic agents. Moreover, the MSN systems seem to be an efficient strategy to further help to decrease treatment costs by reducing the drug dose.
Recent Advances in Nanoparticles-Based Platforms Targeting the PD-1/PD-L1 Pathway for Cancer Treatment
Authors: Yu Xin , Fang Chao , Zhang Kun , Su Chunxia
Published: August 2022

Immune checkpoint inhibitors (ICIs) targeting the PD-1/PD-L1 axis showed remarkable improvements in overall response and patient survival, which changed the treatment landscape for multiple cancer types. However, the majority of patients receiving ICIs are either non-responders or eventually develop secondary resistance. Meanwhile, immunological homeostasis would be destroyed as T cell functions are activated excessively, leading to immune-related adverse events (irAEs). Clinically, a large number of irAEs caused by ICIs occurred and affected almost every organ system, resulting in the discontinuation or even the termination of the ongoing therapy. Therefore, researchers are exploring methods to overcome the situations of insufficient accumulation of these drugs in tumor sites and severe side effects. PD-1/PD-L1-targeted agents encapsulated in nanoparticles have emerged as novel drug delivery systems for improving the delivery efficacy, enhancing immune response and minimizing side effects in cancer treatment. Nanocarriers targeting the PD-1/PD-L1 axis showed enhanced functionalities and improved the technical weaknesses based on their reduced off-target effects, biocompatible properties, multifunctional potential and biomimetic modifications. Here, we summarize nanoparticles which are designed to directly target the PD-1/PD-L1 axis. We also discuss the combination of anti-PD-1/PD-L1 agents and other therapies using nanomedicine-based treatments and their anticancer effects, safety issues, and future prospects.
Spray-Dried Cytisine-Loaded Matrices: Development of Transbuccal Sustained-Release Tablets as a Promising Tool in Smoking Cessation Therapy
Authors: Angellotti Giuseppe , Di Prima Giulia , Scarpaci Amalia Giulia , D’Agostino Fabio , Campisi Giuseppina , De Caro Viviana
Published: August 2022

Cytisine (CYT) has emerged as a promising molecule to treat nicotine addiction, since it acts as a partial agonist of nicotinic acetylcholine receptors. However, its unfavorable pharmacokinetic properties lead to multiple administrations per day, reducing the patient’s compliance and increasing the side effects. To overcome these drawbacks, CYT buccal administration is here proposed. Firstly, CYT stability in the buccal environment was assessed and its intrinsic ability to permeate/penetrate the tissue was determined by applying CYT solutions at increasing concentrations. Furthermore, a spray-drying method was selected and optimized as it is an eco-friendly, easily scalable and effective technique to obtain uniform and reproducible CYT-loaded (5% w/w) pharmaceutical powders, which were directly compressed, thus obtaining different buccal delivery systems (BDSs). The obtained BDSs were homogeneous and reproducible and embedded CYT in its amorphous form. The mechanism of CYT release was evaluated in vitro and found to be mainly driven by a Fickian diffusion phenomenon. Predominantly, the ex vivo permeation assays highlighted the ability of the BDSs to enhance CYT permeation, also producing high drug fluxes through the mucosa. Speculative mathematical evaluations based on the already-known CYT pharmacokinetic parameters showed that CYT-loaded BDSs could potentially be sufficient to obtain a therapeutic effect, thus making the reported formulations suitable candidates for further in vivo trials.
Papain-Decorated Mucopenetrating SEDDS: A Tentative Approach to Combat Absorption Issues of Acyclovir via the Oral Route
Authors: Mahmood Arshad , Haneef Rabbia , Al Meslamani Ahmad Z. , Bostanudin Mohammad F. , Sohail Muhammad , Sarfraz Muhammad , Arafat Mosab
Published: August 2022

The aim of the current study was to enhance the oral bioavailability of Acyclovir (ACV) based on the papain-functionalized self-emulsifying drug delivery systems (SEDDS). The optimum control SEDDS formulation comprised of kolliphore (40%), transcutol (30%), propylene glycol (20%) and oleoyl chloride (10%). However, in the targeted SEDDS formulation, oleoyl chloride was replaced with oleoyl chloride-papain (OC-PAP) conjugate that was synthesized via an amide bond formation between the acyl halide groups of oleoyl chloride and the amino group of papain. Prior to adding in the SEDDS formulation, the newly synthesized conjugate was evaluated quantitatively by a Bradford assay that demonstrated 45 µg of papain contents per mg of the conjugate. Moreover, the conjugate formation was qualitatively confirmed through FTIR analysis and thin layer chromatography. ACV (a BCS class III drug) was incorporated into the SEDDS formulations after being hydrophobically ion paired with sodium deoxycholate, thereby making it lipophilic. The drug-loaded formulations were emulsified in the 0.1 M phosphate buffer (pH 6.8) and evaluated in vitro with respect to drug release and rabbit mucosal permeation studies. Both the formulations illustrated a very comparable drug release over a period of 4 h, afterwards, the OC-PAP-based formulation demonstrated a more sustaining effect. The extent of mucus diffusion evaluated via the silicon tube method demonstrated a 4.92-fold and a 1.46-fold higher penetration of the drug, a 3.21-fold and a 1.56-fold higher permeation through the rabbit intestinal mucus layer, and a 22.94-fold and a 2.27-fold higher retention of the drug over the intact mucosa of rabbit intestine, illustrated by OC-PAP-based nanoemulsions compared to the drug-free solution and controlled nanoemulsion, respectively. According to these in vitro results, papain-functionalized SEDDS is a promising approach for the oral delivery of ACV and many other drugs with oral bioavailability issues, however, in vivo studies in this respect have to be employed before making a comprehensive conclusion.
Antiparasitic Activity of Tea Tree Oil (TTO) and Its Components against Medically Important Ectoparasites: A Systematic Review
Authors: Bezabh Solomon Abrha , Tesfaye Wubshet , Christenson Julia K. , Carson Christine F. , Thomas Jackson
Published: August 2022

Ectoparasites are pathogens that can infect the skin and cause immense pain, discomfort, and disease. They are typically managed with insecticides. However, the fast-emerging antimicrobial resistance and the slow rate of development of new bio-actives combined with environmental and health concerns over the continued use of neurotoxic insecticides warrant newer and alternative methods of control. Tea tree oil (TTO), as an alternative agent, has shown remarkable promise against ectoparasites in recent studies. To our knowledge, this is the first systematic review to assess preclinical and clinical studies exploring the antiparasitic activity of TTO and its components against clinically significant ectoparasites, such as Demodex mites, scabies mites, house dust mites, lice, fleas, chiggers, and bed bugs. We systematically searched databases, including PubMed, MEDLINE (EBSCOhost), Embase (Scopus), CENTRAL, Cochrane Library, CINAHL, ScienceDirect, Web of Science, SciELO, and LILACS in any language from inception to 4 April 2022. Studies exploring the therapeutic activity of TTO and its components against the ectoparasites were eligible. We used the ToxRTool (Toxicological data reliability assessment) tool, the Joanna Briggs Institute (JBI) critical appraisal tools, and the Jadad scale to assess the methodological qualities of preclinical (in vitro and in vivo) studies, non-randomised controlled trials (including cohort, case series, and case studies), and randomised controlled trials, respectively. Of 497 identified records, 71 studies were included in this systematic review, and most (66%) had high methodological quality. The findings of this review revealed the promising efficacy of TTO and its components against ectoparasites of medical importance. Most importantly, the compelling in vitro activity of TTO against ectoparasites noted in this review seems to have translated well into the clinical environment. The promising outcomes observed in clinical studies provide enough evidence to justify the use of TTO in the pharmacotherapy of ectoparasitic infections.
Genomic and Functional Characterization of Vancomycin-Resistant Enterococci-Specific Bacteriophages in the Galleria mellonella Wax Moth Larvae Model
Authors: El Haddad Lynn , Angelidakis Georgios , Clark Justin R. , Mendoza Jesus F. , Terwilliger Austen L. , Chaftari Christopher P. , Duna Mark , Yusuf Serena T. , Harb Cynthia P. , Stibich Mark , Maresso Anthony , Chemaly Roy F.
Published: August 2022

Phages are naturally occurring viruses that selectively kill bacterial species without disturbing the individual’s normal flora, averting the collateral damage of antimicrobial usage. The safety and the effectiveness of phages have been mainly confirmed in the food industry as well as in animal models. In this study, we report on the successful isolation of phages specific to Vancomycin-resistant Enterococci, including Enterococcus faecium (VREfm) and Enterococcus faecalis from sewage samples, and demonstrate their efficacy and safety for VREfm infection in the greater wax moth Galleria mellonella model. No virulence-associated genes, antibiotic resistance genes or integrases were detected in the phages’ genomes, rendering them safe to be used in an in vivo model. Phages may be considered as potential agents for therapy for bacterial infections secondary to multidrug-resistant organisms such as VREfm.
Endocrine and Metabolic Impact of Oral Ingestion of a Carob-Pod-Derived Natural-Syrup-Containing D-Pinitol: Potential Use as a Novel Sweetener in Diabetes
Authors: Navarro Juan A. , Decara Juan , Medina-Vera Dina , Tovar Ruben , Lopez-Gambero Antonio J. , Suarez Juan , Pavón Francisco Javier , Serrano Antonia , de Ceglia Marialuisa , Sanjuan Carlos , Baltasar Yolanda Alfonso , Baixeras Elena , Rodríguez de Fonseca Fernando
Published: August 2022

The widespread use of added sugars or non-nutritive sweeteners in processed foods is a challenge for addressing the therapeutics of obesity and diabetes. Both types of sweeteners generate health problems, and both are being blamed for multiple complications associated with these prevalent diseases. As an example, fructose is proven to contribute to obesity and liver steatosis, while non-nutritive sweeteners generate gut dysbiosis that complicates the metabolic control exerted by the liver. The present work explores an alternative approach for sweetening through the use of a simple carob-pod-derived syrup. This sweetener consists of a balanced mixture of fructose (47%) and glucose (45%), as sweetening sugars, and a functional natural ingredient (D-Pinitol) at a concentration (3%) capable of producing active metabolic effects. The administration of this syrup to healthy volunteers (50 g of total carbohydrates) resulted in less persistent glucose excursions, a lower insulin response to the hyperglycemia produced by its ingestion, and an enhanced glucagon/insulin ratio, compared to that observed after the ingestion of 50 g of glucose. Daily administration of the syrup to Wistar rats for 10 days lowered fat depots in the liver, reduced liver glycogen, promoted fat oxidation, and was devoid of toxic effects. In addition, this repeated administration of the syrup improved glucose handling after a glucose (2 g/kg) load. Overall, this alternative functional sweetener retains the natural palatability of a glucose/fructose syrup while displaying beneficial metabolic effects that might serve to protect against the progression towards complicated obesity, especially the development of liver steatosis.
Candidacidal and Antibiofilm Activity of PS1-3 Peptide against Drug-Resistant Candida albicans on Contact Lenses
Authors: Lee Jong-Kook , Park Soyoung , Kim Young-Min , Guk Taeuk , Lee Min-Young , Park Seong-Cheol , Lee Jung Ro , Jang Mi-Kyeong
Published: August 2022

The recent emergence of antibiotic-resistant fungi has accelerated research on novel antifungal agents. In particular, Candida albicans infections are related to biofilm formation on medical devices, such as catheters, stents, and contact lenses, resulting in high morbidity and mortality. In this study, we aimed to elucidate the antifungal and antibiofilm effects of a peptide against drug-resistant C. albicans. α-Helical peptides in which the sequence of KWYK was repeated twice and four times, designated peptide series 1 (PS1)-1 and PS1-3, respectively, were generated, and the candidacidal activities of PS1-1, PS1-3, and fluconazole against drug-resistant C. albicans cells were assessed. The PS1-3 peptide showed higher killing activity than PS1-1 or fluconazole and acted via a membranolytic mechanism. In addition, the PS1-3 peptide exhibited more potent activity than PS1-1 and fluconazole in terms of fungal biofilm inhibition and reduction at the minimum fungicidal concentration on the contact lens surface. Overall, these findings established PS1-3 as a potential candidacidal agent for applications on contact lenses.
Transcriptional Comparison of New Hybrid Progenies and Clone-Cultivars of Tea (Camellia sinensis L.) Associated to Catechins Content
Authors: Widhianata Hani , Basunanda Panjisakti , Supriyadi Supriyadi , Taryono Taryono
Published: August 2022

Heterosis or hybrid vigor is the improved performance of a desirable quality in hybrid progeny. Hybridization between high-productive Assam type and high-quality Chinese type clone-cultivar is expected to develop elite tea plant progenies with high quality and productivity. Comparative transcriptomics analyses of leaves from the F1 hybrids and their parental clone-cultivars were conducted to explore molecular mechanisms related to catechin content using a high-throughput next-generation RNA-seq strategy and high-performance liquid chromatography (HPLC). The content of EGCG (epigallocatechin gallate) and C (catechin) was higher in ‘Kiara-8’ × ‘Sukoi’, ‘Tambi-2’ × ‘Suka Ati’, and ‘Tambi-2’ × ‘TRI-2025’ than the other hybrid and clone-cultivars. KEGG (Kyoto Encyclopedia of Genes and Genomes) and GO (Gene Ontology) analysis found that most pathways associated with catechins content were enriched. Significant differentially expressed genes (DEGs) mainly associated with phenylpropanoid, flavonoid, drug metabolism-cytochrome P450, and transcription factor (MYB, bHLH, LOB, and C2H2) pathways appeared to be responsible for the high accumulation of secondary metabolites in ‘Kiara-8’ × ‘Sukoi’, ‘Tambi-2’ × ‘Suka Ati’, and ‘Tambi-2’ × ‘TRI-2025’ as were detected in EGCG and catechin content. Several structural genes related to the above pathways have been obtained, which will be used as candidate genes in the screening of breeding materials.
Periodicity and Spectral Composition of Light in the Regulation of Hypocotyl Elongation of Sunflower Seedlings
Authors: Vinterhalter Dragan , Vinterhalter Branka , Motyka Vaclav
Published: August 2022

This study presents the hypocotyl elongation of sunflower seedlings germinated under different light conditions. Elongation was rhythmic under diurnal (LD) photoperiods but uniform (arrhythmic) under free-running conditions of white light (LL) or darkness (DD). On the sixth day after the onset of germination, seedlings were entrained in all diurnal photoperiods. Their hypocotyl elongation was dual, showing different kinetics in daytime and nighttime periods. The daytime elongation peak was around midday and 1–2 h after dusk in the nighttime. Plantlets compensated for the differences in the daytime and nighttime durations and exhibited similar overall elongation rates, centered around the uniform elongation in LL conditions. Thus, plants from diurnal photoperiods and LL could be grouped together as white-light treatments that suppressed hypocotyl elongation. Hypocotyl elongation was significantly higher under DD than under white-light photoperiods. In continuous monochromatic blue, yellow, green, or red light, hypocotyl elongation was also uniform and very high. The treatments with monochromatic light and DD had similar overall elongation rates; thus, they could be grouped together. Compared with white light, monochromatic light promoted hypocotyl elongation. Suppression of hypocotyl elongation and rhythmicity reappeared in some combination with two or more monochromatic light colors. The presence of red light was obligatory for this suppression. Plantlets entrained in diurnal photoperiods readily slipped from rhythmic into uniform elongation if they encountered any kind of free-running conditions. These transitions occurred whenever the anticipated duration of daytime or nighttime was extended more than expected, or when plantlets were exposed to constant monochromatic light. This study revealed significant differences in the development of sunflower plantlets illuminated with monochromatic or white light.
Genome-Wide Association Study Reveals the Genetic Basis of Kernel and Cob Moisture Changes in Maize at Physiological Maturity Stage
Authors: Zhang Minyan , Xiangchen Chaoyang , Yan Jiaquan , Chengxu Yujuan , Liu Hao , Zou Chaoying , Pan Guangtang , Shen Yaou , Ma Langlang
Published: August 2022

Low moisture content (MC) and high dehydration rate (DR) at physiological maturity affect grain mechanical harvest, transport, and storage. In this study, we used an association panel composed of 241 maize inbred lines to analyze ear moisture changes at physiological maturity stage. A genome-wide association study revealed nine significant SNPs and 91 candidate genes. One SNP (SYN38588) was repeatedly detected for two traits, and 15 candidate genes were scanned in the linkage disequilibrium regions of this SNP. Of these, genes Zm00001d020615 and Zm00001d020623 were individually annotated as a polygalacturonase (PG) and a copper transporter 5.1 (COPT5.1), respectively. Candidate gene association analysis showed that three SNPs located in the exons of Zm00001d020615 were significantly associated with the dehydration rate, and AATTAA was determined as the superior haplotype. All these findings suggested that Zm00001d020615 was a key gene affecting moisture changes of maize at the physiological maturity stage. These results have demonstrated the genetic basis of ear moisture changes in maize and indicated a superior haplotype for cultivating maize varieties with low moisture content and high dehydration rates.
Bio-Stimulating Effect of Natural Polysaccharides from Lobularia maritima on Durum Wheat Seedlings: Improved Plant Growth, Salt Stress Tolerance by Modulating Biochemical Responses and Ion Homeostasis
Authors: Bouteraa Mohamed Taieb , Mishra Avinash , Romdhane Walid Ben , Hsouna Anis Ben , Siddique Kadambot H. M. , Saad Rania Ben
Published: August 2022

Bioactivities of polysaccharides derived from halophyte plants have gained attention in recent years. The use of biostimulants in agriculture is an innovative method of dealing with environmental stressors affecting plant growth and development. Here, we investigated the use of natural polysaccharides derived from the halophyte plant Lobularia maritima (PSLm) as a biostimulant in durum wheat seedlings under salt stress. Treatment with polysaccharide extract (0.5, 1, and 2 mg/mL PSLm) stimulated in vitro wheat growth, including germination, shoot length, root length, and fresh weight. PSLm at 2 mg/mL provided tolerance to plants against NaCl stress with improved membrane stability and low electrolyte leakage, increased antioxidant activities (catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD)), enhanced leaf chlorophyll fluorescence, proline, and total sugar contents, decreased lipid peroxidation (MDA), and reactive oxygen species (H2O2) levels, and coordinated the efflux and compartmentation of intracellular ions. The expression profile analyses of ten stress-related genes (NHX1, HKT1.4, SOS1, SOD, CAT, GA20-ox1, GA3-ox1, NRT1.1, NRT2.1, and GS) using RT-qPCR revealed the induction of several key genes in durum wheat growing in media supplemented with PSLm extract, even in unstressed conditions that could be related to the observed tolerance. This study revealed that PSLm extract contributes to salt tolerance in durum wheat seedlings, thereby enhancing their reactive oxygen species scavenging ability, and provided evidence for exploring PSLm as a plant biostimulant for sustainable and organic agriculture.
Genome-Wide Transcriptomic Analysis of the Effects of Infection with the Hemibiotrophic Fungus Colletotrichum lindemuthianum on Common Bean
Authors: Alvarez-Diaz Juan C. , Laugé Richard , Delannoy Etienne , Huguet Stéphanie , Paysant-Le Roux Christine , Gratias Ariane , Geffroy Valérie
Published: August 2022

Bean anthracnose caused by the hemibiotrophic fungus Colletotrichum lindemuthianum is one of the most important diseases of common bean (Phaseolus vulgaris) in the world. In the present study, the whole transcriptome of common bean infected with C. lindemuthianum during compatible and incompatible interactions was characterized at 48 and 72 hpi, corresponding to the biotrophy phase of the infection cycle. Our results highlight the prominent role of pathogenesis-related (PR) genes from the PR10/Bet vI family as well as a complex interplay of different plant hormone pathways including Ethylene, Salicylic acid (SA) and Jasmonic acid pathways. Gene Ontology enrichment analysis reveals that infected common bean seedlings responded by down-regulation of photosynthesis, ubiquitination-mediated proteolysis and cell wall modifications. In infected common bean, SA biosynthesis seems to be based on the PAL pathway instead of the ICS pathway, contrarily to what is described in Arabidopsis. Interestingly, ~30 NLR were up-regulated in both contexts. Overall, our results suggest that the difference between the compatible and incompatible reaction is more a question of timing and strength, than a massive difference in differentially expressed genes between these two contexts. Finally, we used RT-qPCR to validate the expression patterns of several genes, and the results showed an excellent agreement with deep sequencing.
Comparison of Antifungal Activity of Bacillus Strains against Fusarium graminearum In Vitro and In Planta
Authors: Jimenez-Quiros Catherine , Okechukwu Emeka C. , Hong Yiguo , Baysal Ömür , Tör Mahmut
Published: August 2022

Fusarium graminearum (Fg) causes Fusarium head blight (FHB) disease in wheat and barley. This pathogen produces mycotoxins including deoxynivalenol (DON), the T-2 and fumorisin B1. Translocation of the mycotoxins in grains causes important losses in yields and contributes to serious health problems in humans and livestock. We tested the Bacillus strains, two commercial, QST713 (Serenade®) and FZB24 (TAEGRO®) and one non-commercial strain EU07 as microbial biological control agents against the F. graminearum strain Fg-K1-4 both in vitro and in planta. The EU07 strain showed better performance in suppressing the growth of Fg-K1-4. Cell-free bacterial cultures displayed significant antagonistic activity on Fg-K1-4. Remarkably, heat and proteinase K treatment of bacterial broths did not reduce the antagonistic activity of Bacillus cultures. DON assays showed that Bacillus strain was not affected by the presence of DON in the media. Leaf and head infection assays using Brachypodium distachyon (Bd-21) indicated that EU07 inhibits Fg-K1-4 growth in vivo and promotes plant growth. Overall, the EU07 strain performed better, indicating that it could be explored for the molecular investigations and protection of cereal crops against FHB disease.
Effect of Melatonin in Broccoli Postharvest and Possible Melatonin Ingestion Level
Authors: Cano Antonio , Giraldo-Acosta Manuela , García-Sánchez Sara , Hernández-Ruiz Josefa , B. Arnao Marino
Published: August 2022

The post-harvest stage of broccoli production requires cold storage to obtain enough days of shelf life. It has been proved that melatonin is useful as a post-harvest agent in fruits and vegetables, including broccoli. In this study, the broccoli heads treated with melatonin have a longer shelf life than the control samples, which was reflected in parameters such as fresh weight, hue angle (expresses color quality), and chlorophyll and carotenoid contents. Treatments with 100 μM melatonin for 15 or 30 min seem to be the most appropriate, extending the broccoli’s shelf life to almost 42 days, when it is normally around 4 weeks. In addition, a study on the possible impact that melatonin treatments in broccoli could have on melatonin intake in humans is presented. The levels of superficial melatonin, called washing or residual melatonin, are measured, showing the possible incidence in estimated blood melatonin levels. Our results suggest that post-harvest treatments with melatonin do not have to be a handicap from a nutritional point of view, but more research is needed.
Pharmaceutical Potential of High-Altitude Plants for Fatigue-Related Disorders: A Review
Authors: Zhu Hongkang , Liu Chang , Qian He
Published: August 2022

Natural plants from plateaus have been the richest source of secondary metabolites extensively used in traditional and modern health care systems. They were submitted to years of natural selection, co-evolved within that habitat, and show significant anti-fatigue-related pharmacological effects. However, currently, no review on high-altitude plants with anti-fatigue related properties has been published yet. This study summarized several Chinese traditional high-altitude plants, including Rhodiola rosea L., Crocus sativus L., Lepidium meyenii W., Hippophaerhamnoides L., which are widely used in the Qinghai–Tibet Plateau and surrounding mountains, as well as herbal markets in the plains. Based on phytopharmacology studies, deeper questions can be further revealed regarding how these plants regulate fatigue and related mental or physical disease conditions. Many active derivatives in high-altitude medical plants show therapeutic potential for the management of fatigue and related disorders. Therefore, high-altitude plants significantly relieve central or peripheral fatigue by acting as neuroprotective agents, energy supplements, metabolism regulators, antioxidant, and inflammatory response inhibitors. Their applications on the highland or flatland and prospects in natural medicine are further forecast, which may open treatments to reduce or prevent fatigue-related disorders in populations with sub-optimal health.
Biochar Particles Obtained from Agricultural Carob Waste as a Suitable Filler for Sustainable Biocomposite Formulations
Authors: Infurna Giulia , Botta Luigi , Maniscalco Marco , Morici Elisabetta , Caputo Giuseppe , Marullo Salvatore , D’Anna Francesca , Dintcheva Nadka Tz.
Published: August 2022

In the context of sustainable and circular economy, the recovery of biowaste for sustainable biocomposites formulation is a challenging issue. The aim of this work is to give a new life to agricultural carob waste after glucose extraction carried out by a local factory for carob candy production. A pyrolysis process was carried out on bio-waste to produce biofuel and, later, the solid residual fraction of pyrolysis process was used as interesting filler for biocomposites production. In this work, biochar particles (BC) as a pyrolysis product, after fuels recovery of organic biowaste, specifically, pyrolyzed carobs after glucose extraction, were added on poly(butylene-adipate-co-terephthalate), (PBAT), at two different concentrations, i.e., 10 and 20 wt%. The BC have been produced using three pyrolysis processing temperatures (i.e., 280, 340 and 400 °C) to optimize the compositions of produced solid fractions and biofuels. The resulting particles from the pyrolysis process (BC280, BC340 and BC400) were considered as suitable fillers for PBAT. Firstly, the BC particles properties were characterized by elemental composition and spectroscopy analysis, particle size measurements and evaluation of radical scavenging activity and efficiency. Moreover, PBAT/BC composites were subjected to analysis of their rheological and thermal behavior, morphologies and mechanical properties. In addition, accelerated weathering, monitored by both tensile test and spectroscopic analysis, was carried out, and obtained results show that the biochar particles can exert a beneficial effect on photo-oxidation delay of PBAT matrix.
Co/ZnO/Nitrogen-Doped Carbon Composite Anode Derived from Metal Organic Frameworks for Lithium Ion Batteries
Authors: Chang Ya-Chun , Huang Chia-Hung , Liu Wei-Ren
Published: August 2022

Through high-temperature sintering and carbonization, two Co/ZnO nitrogen-doped porous carbon (NC) composites derived from ZIF-8 and ZIF-67 were manufactured for use as anodes for Li ion batteries: composite-type Co/ZnO-NC and core-shell-type Co@ZnO-NC. X-ray diffraction analysis, scanning electron microscopy, and the Brunauer–Emmett–Teller (BET) method were performed to identify the pore distribution and surface morphology of these composites. The findings of the BET method indicated that the specific surface area of Co/ZnO-NC was 350 m2/g, which was twice that of Co@ZnO-NC. Electrochemical measurements revealed that Co@ZnO-NC and Co/ZnO-NC had specific capacities of over 400 mAh g−1 at a current density 0.2 A g−1 after 50 cycles. After 100 cycles, Co/ZnO-NC exhibited a reversible capacity of 411 mAh g−1 at a current density of 0.2 A g−1 and Co@ZnO-NC had a reversible capacity of 246 mAh g−1 at a current density of 0.2 A g−1. The results indicated that Co/ZnO-NC exhibited superior electrochemical performance to Co@ZnO-NC as a potential anode for use in Li ion batteries.
Properties and Fabrication of Waterborne Polyurethane Superhydrophobic Conductive Composites with Coupling Agent-Modified Fillers
Authors: Wang Fangfang , Ci Jihao , Fan Jiang
Published: August 2022

The addition of abundant fillers to obtain conductive and superhydrophobic waterborne polyurethane (WPU) composites generally results in increased interfaces in the composites, leading to reduced adhesion and poor corrosion resistance. Fillers such as Polytetrafluoroethylene (PTFE) and multi-walled carbon nanotubes (MWCNTs) were first treated by a coupling agent to reduce the contents of the fillers. Thus, in this work, WPU superhydrophobic conductive composites were prepared using electrostatic spraying (EsS). The polar groups (-OH and -COOH, etc.) on the WPU, PTFE, and MWCNTs were reacted with the coupling agent, making the WPU, PTFE, and MWCNTs become crosslinked together. Thus, the uniformity of the coating was improved and its curing interfaces were reduced, causing enhanced corrosion resistance. The dehydration reaction that occurred between the silane coupling agent and the polar surface of Fe formed -NH2 groups, increasing the adhesion of the coating to the steel substrate and then solving the problems of low adhesion, easy delamination, and exfoliation. With the increased content of the modified fillers, the conductivity and hydrophobic property of the composite were amplified, and its corrosion resistance and adhesion were first strengthened and then declined. The composite with the WPU, PTFE, MWCNTs, and KH-550 at a mass ratio of 7:1.5:0.1:0.032 held excellent properties; its volume resistivity and WCA were 1.5 × 104 Ω·cm and 155°, respectively. Compared with the pure WPU coating, its adhesive and anticorrosive properties were both better. This provides a foundation for the fabrication and application of anticorrosive and conductive waterborne composites.
A Hybrid SVR-Based Prediction Model for the Interfacial Bond Strength of Externally Bonded FRP Laminates on Grooves with Concrete Prisms
Authors: Khan Kaffayatullah , Iqbal Mudassir , Biswas Rahul , Amin Muhammad Nasir , Ali Sajid , Gudainiyan Jitendra , Alabdullah Anas Abdulalim , Arab Abdullah Mohammad Abu
Published: August 2022

The current work presents a comparative study of hybrid models that use support vector machines (SVMs) and meta-heuristic optimization algorithms (MOAs) to predict the ultimate interfacial bond strength (IBS) capacity of fiber-reinforced polymer (FRP). More precisely, a dataset containing 136 experimental tests was first collected from the available literature for the development of hybrid SVM models. Five MOAs, namely the particle swarm optimization, the grey wolf optimizer, the equilibrium optimizer, the Harris hawks optimization and the slime mold algorithm, were used; five hybrid SVMs were constructed. The performance of the developed SVMs was then evaluated. The accuracy of the constructed hybrid models was found to be on the higher side, with R2 ranges between 0.8870 and 0.9774 in the training phase and between 0.8270 and 0.9294 in the testing phase. Based on the experimental results, the developed SVM–HHO (a hybrid model that uses an SVM and the Harris hawks optimization) was overall the most accurate model, with R2 values of 0.9241 and 0.9241 in the training and testing phases, respectively. Experimental results also demonstrate that the developed hybrid SVM can be used as an alternate tool for estimating the ultimate IBS capacity of FRP concrete in civil engineering projects.
Humic Acid-Coated Fe3O4 Nanoparticles Confer Resistance to Acremonium Wilt Disease and Improve Physiological and Morphological Attributes of Grain Sorghum
Authors: El-Ganainy Sherif Mohamed , El-Bakery Amal M. , Hafez Heba M. , Ismail Ahmed Mahmoud , El-Abdeen Ali Zein , Ata Abed Abd Elgalel , Elraheem Omar A. Y. Abd , El Kady Yousef M. Y. , Hamouda Ahlam F. , El-Beltagi Hossam S. , Shehata Wael F. , Shalaby Tarek A. , Abbas Ahmed Osman , Almaghsla Mustafa Ibrahim , Sattar Muhammad N. , Iqbal Zafar
Published: August 2022

Acremonium wilt disease affects grain quality and reduces sorghum yield around the globe. The present study aimed to assess the efficacy of humic acid (HA)-coated Fe3O4 (Fe3O4/HA) nanoparticles (NPs) in controlling acremonium wilt disease and improving sorghum growth and yields. During the season 2019, twenty-one sorghum genotypes were screened to assess their response to Acremonium striticum via artificial infection under field conditions and each genotype was assigned to one of six groups, ranging from highly susceptible to highly resistant. Subsequently, over the two successive seasons 2020 and 2021, three different concentrations of 10, 40 and 80 mg L−1 of Fe3O4/HA NPs were tested against A. striticum. The concentrations of 40 and 80 mg L−1 were found to be highly effective in controlling acremonium wilt disease on different sorghum genotypes: LG1 (highly susceptible), Giza-3 (susceptible), and Local 119 (resistant) genotypes. After harvest, the physiological (growth and yield) and biochemical (peroxidase, catalase, and gibberellic acid) attributes of sorghum plants were determined, and the results demonstrated that concentrations of 40 and 80 mg L−1 increased peroxidase and catalase activities in healthy (uninoculated) sorghum genotypes compared to inoculated sorghum genotypes. Additionally, the toxicity of Fe3O4/HA NPs on male albino rats was investigated via hematological (CBC), chemical (ALT and AST) and histopathological analyses. The concentration 80 mg L−1 of Fe3O4/HA NPs caused a marked increase in ALT and creatinine level after 51 days of feeding. Severe pathological alterations were also observed in liver and kidney tissues of rats administered with grain sorghums treated with 80 mg L−1. In comparison with the untreated control plants, a concentration of 40 mg L−1 significantly increased the growth, yield and gibberellic acid levels (p ≤ 0.05) and was found to be safe in male albino rats. Conclusively, a concentration of 40 mg L−1 of Fe3O4/HA NPs showed promising results in curtailing A. striticum infections in sorghum, indicating its great potential to substitute harmful fertilizers and fungicides as a smart agriculture strategy.
Strain Release Behaviour during Crack Growth of a Polymeric Beam under Elastic Loads for Self-Healing
Authors: Almutairi Mohammed Dukhi , Alnahdi Sultan Saleh , Khan Muhammad A.
Published: August 2022

The response of polymeric beams made of Acrylonitrile butadiene styrene (ABS) and thermoplastic polyurethane (TPU) in the form of 3D printed beams is investigated to test their elastic and plastic responses under different bending loads. Two types of 3D printed beams were designed to test their elastic and plastic responses under different bending loads. These responses were used to develop an origami capsule-based novel self-healing mechanism that can be triggered by crack propagation due to strain release in a structure. Origami capsules of TPU in the form of a cross with four small beams, either folded or elastically deformed, were embedded in a simple ABS beam. Crack propagation in the ABS beam released the strain, and the TPU capsule unfolded with the arms of the cross in the direction of the crack path, and this increased the crack resistance of the ABS beam. This increase in the crack resistance was validated in a delamination test of a double cantilever specimen under quasi-static load conditions. Repeated test results demonstrated the effect of self-healing on structural crack growth. The results show the potential of the proposed self-healing mechanism as a novel contribution to existing practices which are primarily based on external healing agents.
Influence of Processing Glass-Fiber Filled Plastics on Different Twin-Screw Extruders and Varying Screw Designs on Fiber Length and Particle Distribution
Authors: Rüppel Annette , Wolff Susanne , Oldemeier Jan Philipp , Schöppner Volker , Heim Hans-Peter
Published: August 2022

Due to their valuable properties (low weight, and good thermal and mechanical properties), glass fiber reinforced thermoplastics are becoming increasingly important. Fiber-reinforced thermoplastics are mainly manufactured by injection molding and extrusion, whereby the extrusion compounding process is primarily used to produce fiber-filled granulates. Reproducible production of high-quality components requires a granulate in which the fiber length is even and high. However, the extrusion process leads to the fact that fiber breakages can occur during processing. To enable a significant quality enhancement, experimentally validated modeling is required. In this study, short glass fiber reinforced thermoplastics (polypropylene) were produced on two different twin-screw extruders. Therefore, the machine-specific process behavior is of major interest regarding its influence. First, the fiber length change after processing was determined by experimental investigations and then simulated with the SIGMA simulation software. By comparing the simulation and experimental tests, important insights could be gained and the effects on fiber lengths could be determined in advance. The resulting fiber lengths and distributions were different, not only for different screw configurations (SC), but also for the same screw configurations on different twin-screw extruders. This may have been due to manufacturer-specific tolerances.
Influence of Printing Parameters on Self-Cleaning Properties of 3D Printed Polymeric Fabrics
Authors: Atwah Ayat Adnan , Almutairi Mohammed Dukhi , He Feiyang , Khan Muhammad A.
Published: August 2022

The processes for making self-cleaning textile fabrics have been extensively discussed in the literature. However, the exploration of the potential for self-cleaning by controlling the fabrication parameters of the fabric at the microscopic level has not been addressed. The current evolution in 3D printing technology provides an opportunity to control parameters during fabric manufacturing and generate self-cleaning features at the woven structural level. Fabrication of 3D printed textile fabrics using the low-cost fused filament fabrication (FFF) technique has been achieved. Printing parameters such as orientation angle, layer height, and extruder width were used to control self-cleaning microscopic features in the printed fabrics. Self-cleaning features such as surface roughness, wettability contact angle, and porosity were analyzed for different values of printing parameters. The combination of three printing parameters was adjusted to provide the best self-cleaning textile fabric surface: layer height (LH) (0.15, 0.13, 0.10 mm) and extruder width (EW) (0.5, 0.4, 0.3 mm) along with two different angular printing orientations (O) (45° and 90°). Three different thermoplastic flexible filaments printing materials were used: thermoplastic polyurethane (TPU 98A), thermoplastic elastomers (TPE felaflex), and thermoplastic co-polyester (TPC flex45). Self-cleaning properties were quantified using a pre-set defined criterion. The optimization of printing parameters was modeled to achieve the best self-cleaning features for the printed specimens.
Church-Franchise: Missional Innovation for Church Planting and Leadership Mentorship in Neo-Pentecostal and Neo-Prophetic Churches in Africa
Authors: White Peter , Pondani Simbarashe
Published: August 2022

The great commission, as traditionally understood in Christendom, has been the core basis for church growth. Passion for the lost, evangelism outreach, conversions, and baptism followed by congregating, have culminated in church establishments. Pentecostals, especially the Neo-Pentecostal and Neo-Prophetic Churches have attracted huge crowds. The thrust towards fulling the Missio Dei has seen micro–Neo-Pentecostal Churches and Neo-Prophetic Churches in Africa becoming enormous ministries over a short period of time. However, growth within Neo-Pentecostalism and Neo-Prophetism in the African setting has been associated with all kinds of challenges: leadership crisis, fund misuse, and a lack of accountability. Furthermore, controversy with regard to some of the Neo-Pentecostal and Neo-Prophetic leaders running these ‘churches’, has made headlines on several accounts within the social media space. It seems that there is a lack of leadership mentorship as many of them do not belong to a denominational body or lack the idea of ‘belonging’. In view of the aforementioned reasons and other related challenges, this article proposes an innovative missional model of franchising. The thrust of this article is to explore the possibility of church-franchising and how the Nnoboa mission concept proposed by White (2019) offers an alternative framework within which to engage the Neo-Pentecostal and Neo-Prophetic Churches for church planting and leadership mentorship.