bluuebunny/crossref_metadata_2025 huggingface dataset:
Pretraining Convolutional Neural Networks for Mudstone Petrographic Thin-Section Image Classification
Authors: Pires de Lima Rafael , Duarte David
Published: August 2021

Convolutional neural networks (CNN) are currently the most widely used tool for the classification of images, especially if such images have large within- and small between- group variance. Thus, one of the main factors driving the development of CNN models is the creation of large, labelled computer vision datasets, some containing millions of images. Thanks to transfer learning, a technique that modifies a model trained on a primary task to execute a secondary task, the adaptation of CNN models trained on such large datasets has rapidly gained popularity in many fields of science, geosciences included. However, the trade-off between two main components of the transfer learning methodology for geoscience images is still unclear: the difference between the datasets used in the primary and secondary tasks; and the amount of available data for the primary task itself. We evaluate the performance of CNN models pretrained with different types of image datasets—specifically, dermatology, histology, and raw food—that are fine-tuned to the task of petrographic thin-section image classification. Results show that CNN models pretrained on ImageNet achieve higher accuracy due to the larger number of samples, as well as a larger variability in the samples in ImageNet compared to the other datasets evaluated.
The Literary Response to the Holocaust and the Transformation of the Reader into a Messenger
Authors: Patterson David
Published: August 2021

“The greatest mitzvah,” Lily Lerner remembers what her mother taught her, “is to accompany a dead person to burial” (Lerner 1980, p. 35) [...]
Impact of the Introduction of Accreditation Standards on the Satisfaction of Patients in Cardiology Departments
Authors: Mlynarski Rafal , Kaczkowski Rafal , Mlynarska Agnieszka
Published: August 2021

Background: It can be presumed that introducing accreditation standards and obtaining national accreditation by a hospital should translate into increased patient satisfaction. The aim was to analyze the impact of introducing accreditation standards on patient satisfaction in cardiology departments. Methods: 1080 patients, who were hospitalized in four cardiological wards (W1–W4) between 2009–2015, were asked to complete a 22-item questionnaire that assessed the level of their satisfaction with their medical care. 58 questionnaires were excluded because of incomplete data. The hospital was accredited in 2013. Results: In 3 of the 4 wards, a statistically higher total score (the patient was more satisfied) in the period after the accreditation (2013–2015) compared to the period before the accreditation (2009–2012) was as follows: W1 (80.37 ± 6.54 vs. 83.85 ± 5.9; p = 0.0004), W2 (79.95 ± 7.62 vs. 81.46 ± 8.2: p = 0.0376), W4: (78.84 ± 7.94 vs. 84.91 ± 5.57; p = 0.0376); in one ward, there was no statistical difference: W3 (80.11 ± 8.42 vs. 81.07 ± 8.15; p = 0.3284). A significant difference was found in the number of points for the total assessment that were collected for all of the analyzed departments throughout the entire period (2009–2015)–W1: p = 0.0032; W2: p = 0.0176; W3: p = 0.0313 and W4: p < 0.0001). The medium-term rate of the change of the total score decreased after the accreditation. Conclusion: Preparing a hospital for a national accreditation program brought significant benefits for patients in a long-term observation.
Reply to van den Broek et al. Comment on “Laratta et al. Marital Stability and Quality of Couple Relationships after Acquired Brain Injury: A Two-Year Follow-Up Clinical Study. Healthcare 2021, 9, 283”
Authors: Calabrò Rocco Salvatore , Laratta Stefania , Cerasa Antonio
Published: August 2021

We thank van den Broek et al. [...]
Performance, Health, and Psychological Challenges Faced by Students of Physical Education in Online Learning during COVID-19 Epidemic: A Qualitative Study in China
Authors: Laar Rizwan Ahmed , Ashraf Muhammad Azeem , Ning Jin , Ji Peigang , Fang Ping , Yu Tianran , Khan Muhammad Naeem
Published: August 2021

The spread of COVID-19 has led students to take classes online (rather than face-to-face) worldwide, including in China. For this study, we conducted qualitative focus group discussions to identify the experience of and difficulties faced by online physical education students in higher education taking online classes, including the impact on their physical activity performance, as well as some health problems they face while taking online classes during the quarantine period, such as weight gain, depression, and anxiety. Finally, utilizing Mayer’s learning model as a conceptual framework, we propose a method that addresses how to effectively manage an optimal future learning system for physical education students, both during and after the COVID-19 outbreak situation. During the isolation period of COVID-19, the required equipment for the participants was rarely available at home while attending the online classes, which inevitably reduced the number of physical education units that could be conveyed. This resulted in a transfer of attention from competition to underperformance, weight gain, and adverse psychological conditions. We conclude that it is important to review and systematize the methods of online physical education learning, particularly highlighting the cultural and educational characteristics of different countries, and to investigate the effectiveness of online physical education as a whole.
Urban Overheating Assessment through Prediction of Surface Temperatures: A Case Study of Karachi, Pakistan
Authors: Aslam Bilal , Maqsoom Ahsen , Khalid Nauman , Ullah Fahim , Sepasgozar Samad
Published: August 2021

Global climate has been radically affected by the urbanization process in recent years. Karachi, Pakistan’s economic hub, is also showing signs of swift urbanization. Owing to the construction of infrastructure projects under the China-Pakistan Economic Corridor (CPEC) and associated urbanization, Karachi’s climate has been significantly affected. The associated replacement of natural surfaces by anthropogenic materials results in urban overheating and increased local temperatures leading to serious health issues and higher air pollution. Thus, these temperature changes and urban overheating effects must be addressed to minimize their impact on the city’s population. For analyzing the urban overheating of Karachi city, LST (land surface temperature) is assessed in the current study, where data of the past 20 years (2000–2020) is used. For this purpose, remote sensing data from the Advanced Spaceborne Thermal Emission and Reflection Radiometer Global Digital Elevation Model (ASTER GDEM) and Moderate-Resolution Imaging Spectroradiometer (MODIS) sensors were utilized. The long short-term memory (LSTM) model was utilized where the road density (RD), elevation, and enhanced vegetation index (EVI) are used as input parameters. Upon comparing estimated and measured LST, the values of mean absolute error (MAE), mean square error (MSE), and mean absolute percentage error (MAPE) are 0.27 K, 0.237, and 0.15% for January, and 0.29 K, 0.261, and 0.13% for May, respectively. The low MAE, MSE, and MAPE values show a higher correlation between the predicted and observed LST values. Moreover, results show that more than 90% of the pixel data falls in the least possible error range of −1 K to +1 K. The MAE, MSE and MAPE values for Support Vector Regression (SVR) are 0.52 K, 0.453 and 0.18% and 0.76 K, 0.873, and 0.26%. The current model outperforms previous studies, shows a higher accuracy, and depicts greater reliability to predict the actual scenario. In the future, based on the accurate LST results from this model, city planners can propose mitigation strategies to reduce the harmful effects of urban overheating and associated Urban Heat Island effects (UHI).
Treatment of Neuromyelitis Optica Spectrum Disorders
Authors: Chan Koon-Ho , Lee Chi-Yan
Published: August 2021

Neuromyelitis optica spectrum disorder (NMOSD) is an autoimmune central nervous system (CNS) inflammatory disorder that can lead to serious disability and mortality. Females are predominantly affected, including those within the reproductive age. Most patients develop relapsing attacks of optic neuritis; longitudinally extensive transverse myelitis; and encephalitis, especially brainstem encephalitis. The majority of NMOSD patients are seropositive for IgG autoantibodies against the water channel protein aquaporin-4 (AQP4-IgG), reflecting underlying aquaporin-4 autoimmunity. Histological findings of the affected CNS tissues of patients from in-vitro and in-vivo studies support that AQP4-IgG is directly pathogenic in NMOSD. It is believed that the binding of AQP4-IgG to CNS aquaporin-4 (abundantly expressed at the endfoot processes of astrocytes) triggers astrocytopathy and neuroinflammation, resulting in acute attacks. These attacks of neuroinflammation can lead to pathologies, including aquaporin-4 loss, astrocytic activation, injury and loss, glutamate excitotoxicity, microglial activation, neuroinflammation, demyelination, and neuronal injury, via both complement-dependent and complement-independent pathophysiological mechanisms. With the increased understanding of these mechanisms underlying this serious autoimmune astrocytopathy, effective treatments for both active attacks and long-term immunosuppression to prevent relapses in NMOSD are increasingly available based on the evidence from retrospective observational data and prospective clinical trials. Knowledge on the indications and potential side effects of these medications are essential for a clear evaluation of the potential benefits and risks to NMOSD patients in a personalized manner. Special issues such as pregnancy and the coexistence of other autoimmune diseases require additional concern and meticulous care. Future directions include the identification of clinically useful biomarkers for the prediction of relapse and monitoring of the therapeutic response, as well as the development of effective medications with minimal side effects, especially opportunistic infections complicated by long-term immunosuppression.
Targeted and Non-Targeted Mechanisms for Killing Hypoxic Tumour Cells—Are There New Avenues for Treatment?
Authors: Apilan Alyssa Gabrielle , Mothersill Carmel
Published: August 2021

Purpose: A major issue in radiotherapy is the relative resistance of hypoxic cells to radiation. Historic approaches to this problem include the use of oxygen mimetic compounds to sensitize tumour cells, which were unsuccessful. This review looks at modern approaches aimed at increasing the efficacy of targeting and radiosensitizing hypoxic tumour microenvironments relative to normal tissues and asks the question of whether non-targeted effects in radiobiology may provide a new “target”. Novel techniques involve the integration of recent technological advancements such as nanotechnology, cell manipulation, and medical imaging. Particularly, the major areas of research discussed in this review include tumour hypoxia imaging through PET imaging to guide carbogen breathing, gold nanoparticles, macrophage-mediated drug delivery systems used for hypoxia-activate prodrugs, and autophagy inhibitors. Furthermore, this review outlines several features of these methods, including the mechanisms of action to induce radiosensitization, the increased accuracy in targeting hypoxic tumour microenvironments relative to normal tissue, preclinical/clinical trials, and future considerations. Conclusions: This review suggests that the four novel tumour hypoxia therapeutics demonstrate compelling evidence that these techniques can serve as powerful tools to increase targeting efficacy and radiosensitizing hypoxic tumour microenvironments relative to normal tissue. Each technique uses a different way to manipulate the therapeutic ratio, which we have labelled “oxygenate, target, use, and digest”. In addition, by focusing on emerging non-targeted and out-of-field effects, new umbrella targets are identified, which instead of sensitizing hypoxic cells, seek to reduce the radiosensitivity of normal tissues.
Mobile Applications Accessibility: An Evaluation of the Local Portuguese Press
Authors: Santos Gonçalves Tatiana , Ivars-Nicolás Begoña , Martínez-Cano Francisco Julián
Published: August 2021

The local press has always played a central role in the Portuguese society. Recently, new innovative technological projects to develop mobile applications and focus on local journalism in Portugal have emerged. These initiatives allow the development of better and more appealing services for local users. However, due to the important social role of the local press, this also brings along some responsibilities. Our main research goal is to study the accessibility issues in local journalism in Portugal. To this end, we first describe the current situation of local journalism in Portugal and some accessibility issues raised by the appearance of mobile applications. We then develop a simple checklist that allows the assessment of whether these applications have prevented social exclusion and facilitated the access of local information to a wide range of users, including disabled citizens. This tool provides the regional news publisher with information to improve its democratization of access to local information in Portugal. Using the cognitive walkthrough method, we illustrate the proposed framework by presenting case studies of five mobile applications in Portuguese local and regional press. This study concludes that despite the great potential that mobile applications showcase, several accessibility issues have not been properly addressed.
Combined Effects of Pesticides and Electromagnetic-Fields on Honeybees: Multi-Stress Exposure
Authors: Lupi Daniela , Palamara Mesiano Marco , Adani Agnese , Benocci Roberto , Giacchini Roberto , Parenti Paolo , Zambon Giovanni , Lavazza Antonio , Boniotti Maria Beatrice , Bassi Stefano , Colombo Mario , Tremolada Paolo
Published: August 2021

Honeybee and general pollinator decline is extensively reported in many countries, adding new concern to the general biodiversity loss. Many studies were addressed to assess the causes of pollinator decline, concluding that in most cases multi-stress effects were the most probable ones. In this research, the combined effects of two possible stress sources for bees, pesticides and electromagnetic fields (multi-stress conditions), were analyzed in the field. Three experimental sites were chosen: a control one far from direct anthropogenic stress sources, a pesticide-stress site and multi-stress one, adding to the same exposure to pesticides the presence of an electromagnetic field, coming from a high-voltage electric line. Experimental apiaries were monitored weekly for one year (from April 2017 to April 2018) by means of colony survival, queen activity, storage and brood amount, parasites and pathogens, and several biomarkers in young workers and pupae. Both exposure and effect biomarkers were analysed: among the first, acetylcholinesterase (AChE), catalase (CAT), glutathione S-transferase (GST) and alkaline phosphatase (ALP) and Reactive Oxygen Species (ROS); and among the last, DNA fragmentation (DNAFRAGM) and lipid peroxidation (LPO). Results showed that bee health conditions were the worst in the multi-stress site with only one colony alive out of the four ones present at the beginning. In this site, a complex picture of adverse effects was observed, such as disease appearance (American foulbrood), higher mortality in the underbaskets (common to pesticide-stress site), behavioral alterations (queen changes, excess of honey storage) and biochemical anomalies (higher ALP activity at the end of the season). The overall results clearly indicate that the multi-stress conditions were able to induce biochemical, physiological and behavioral alterations which severely threatened bee colony survival.
Fitness Costs of Two Maize Lepidopteran Pests Fed on Bacillus thuringiensis (Bt) Diets Enriched with Vitamins A and C
Authors: López Carmen , Muñoz Pilar , Zanga Daniela , Girón-Calva Patricia Sarai , Eizaguirre Matilde
Published: August 2021

Serious malnutrition problems occur in developing countries where people’s diets are mainly based on staple crops. To alleviate this, high-production crops are being developed that are better adapted to climate change, enriched in micronutrients and vitamins, or resistant to pests. In some cases, new varieties have been developed with several of the characteristics mentioned above, such as biofortified and pest-resistant crops. The development of biofortified Bacillus thuringiensis (Bt) crops raises the question of whether vitamin enrichment of Bt crops can in any way favor those pests that are not very susceptible to the Bt toxin that feed on these crops, such as Helicoverpa armigera (Hübner) or Mythimna unipuncta (Haworth) (Lepidoptera: Noctuidae). In this study, the response to a Bt diet enriched with vitamins A (β-carotene) and C (ascorbic acid) was somewhat different between the two species. M. unipuncta was less sensitive to the toxin than H. armigera, although the ingestion of the Bt diet resulted in oxidative stress (longer larval development and lower pupal weight) which was not mitigated by the vitamins. However, the two vitamins reduced the mortality of H. armigera larvae fed on a Bt-enriched diet; in addition, ß-carotene reduced the activity of the antioxidant glutathione S-transferase (GST) of both species, suggesting it has an antioxidant role. The results obtained here indicate that biofortified Bt crops will not favor the development of H. armigera very much and will not affect M. unipuncta’s development at all, although the effect of the increase in vitamins may be very variable and should be studied for each specific phytophagous.
Resin Use by Stingless Bees: A Review
Authors: Shanahan Maggie , Spivak Marla
Published: August 2021

Stingless bees (Meliponini) are highly social bees that are native to tropical and sub-tropical ecosystems. Resin use is vital to many aspects of stingless bee colony function. Stingless bees use resin to build essential nest structures, repel predators, and kill would-be invaders. Furthermore, resin-derived compounds have been found to enrich the cuticular chemical profiles of many stingless bee species, and resin may play an important role in shaping the microbial communities associated with stingless bees and their nests. Despite its importance for colony function, previous reviews of resin use by stingless bees are lacking. This topic grows increasingly urgent as changes in beekeeping and land use practices occur, potentially diminishing stingless bees’ ability to incorporate resin into the nest environment. In this article, we review existing literature on resin use by stingless bees and discuss potential areas of future research.
Avian Predation in a Declining Outbreak Population of the Spruce Budworm, Choristoneura fumiferana (Lepidoptera: Tortricidae)
Authors: Régnière Jacques , Venier Lisa , Welsh Dan
Published: August 2021

The impact of avian predation on a declining population of the spruce budworm, Choristoneura fumifereana (Clem.), was measured using single-tree exclosure cages in a mature stand of balsam fir, Abies balsamea (L.), and white spruce, Picea glauca (Moench.) Voss. Bird population censuses and observations of foraging and nest-feeding activity were also made to determine the response of budworm-linked warblers to decreasing food availability. Seasonal patterns of foraging. as well as foraging success in the declining prey population was compared to similar information from birds observed in another stand where the spruce budworm population was rising. Avian predation was an important source of mortality between the 4th instar and moth emergence in the declining outbreak population. Mortality by predation increased from negligible to over 98% as budworm density dropped from 100 to <1 larva/kg of host foliage, over 3 years. Calculations based on nest-feeding activity and basic metabolic demands support these observed rates. Seasonal and yearly differences in predation rates observed between the two host-tree species correspond to equivalent shifts in bird foraging behavior in response to dropping insect density. In particular, a preference for searching on white spruce disappeared, although budworm-linked birds remained more efficient at finding food on this plant. The ability to change foraging behavior as prey density dropped differed between bird species.
Diagnosis and Management of Heart Failure in Elderly Patients from Hospital Admission to Discharge: Position Paper
Authors: Damy Thibaud , Chouihed Tahar , Delarche Nicholas , Berrut Gilles , Cacoub Patrice , Henry Patrick , Lamblin Nicholas , Andrès Emmanuel , Hanon Olivier
Published: August 2021

Multidisciplinary management of worsening heart failure (HF) in the elderly improves survival. To ensure patients have access to adequate care, the current HF and French health authority guidelines advise establishing a clearly defined HF patient pathway. This pathway involves coordinating multiple disciplines to manage decompensating HF. Yet, recent registry data indicate that insufficient numbers of patients receive specialised cardiology care, which increases the risk of rehospitalisation and mortality. The patient pathway in France involves three key stages: presentation with decompensated HF, stabilisation within a hospital setting and transitional care back out into the community. In each of these three phases, HF diagnosis, severity and precipitating factors need to be promptly identified and managed. This is particularly pertinent in older, frail patients who may present with atypical symptoms or coexisting comorbidities and for whom geriatric evaluation may be needed or specific geriatric syndrome management implemented. In the transition phase, multi-professional post-discharge management must be coordinated with community health care professionals. When the patient is discharged, HF medication must be optimised, and patients educated about self-care and monitoring symptoms. This review provides practical guidance to clinicians managing worsening HF in the elderly.
An Integrative Systems Biology Approach Identifies Molecular Signatures Associated with Gallbladder Cancer Pathogenesis
Authors: Roy Nabanita , Kshattry Mrinmoy , Mandal Susmita , Jolly Mohit Kumar , Bhattacharyya Dhruba Kumar , Barah Pankaj
Published: August 2021

Gallbladder cancer (GBC) has a lower incidence rate among the population relative to other cancer types but is a major contributor to the total number of biliary tract system cancer cases. GBC is distinguished from other malignancies by its high mortality, marked geographical variation and poor prognosis. To date no systemic targeted therapy is available for GBC. The main objective of this study is to determine the molecular signatures correlated with GBC development using integrative systems level approaches. We performed analysis of publicly available transcriptomic data to identify differentially regulated genes and pathways. Differential co-expression network analysis and transcriptional regulatory network analysis was performed to identify hub genes and hub transcription factors (TFs) associated with GBC pathogenesis and progression. Subsequently, we assessed the epithelial-mesenchymal transition (EMT) status of the hub genes using a combination of three scoring methods. The identified hub genes including, CDC6, MAPK15, CCNB2, BIRC7, L3MBTL1 were found to be regulators of cell cycle components which suggested their potential role in GBC pathogenesis and progression.
Irrational Beliefs and Their Role in Specific and Non-Specific Eating Disorder Symptomatology and Cognitive Reappraisal in Eating Disorders
Authors: Tecuta Lucia , Gardini Valentina , Schumann Romana , Ballardini Donatella , Tomba Elena
Published: August 2021

Background: Research on which specific maladaptive cognitions characterize eating disorders (ED) is lacking. This study explores irrational beliefs (IBs) in ED patients and controls and the association between IBs and ED-specific and non-specific ED symptomatology and cognitive reappraisal. Methods: 79 ED outpatients with anorexia nervosa, bulimia nervosa, or other specified feeding or eating disorders and 95 controls completed the Attitudes and Beliefs Scale-2 (ABS-2) for IBs. ED outpatients also completed the Eating Disorder Inventory-3 (EDI-3) for ED-specific (EDI-3-ED Risk) and non-specific (EDI-3-General Psychological Maladjustment) symptomatology; General Health Questionnaire (GHQ) for general psychopathology; Emotion Regulation Questionnaire (ERQ) for cognitive reappraisal. Results: Multivariate analysis of variance with post hoc comparisons showed that ED outpatients exhibit greater ABS-2-Awfulizing, ABS-2-Negative Global Evaluations, and ABS-2-Low Frustration Tolerance than controls. No differences emerged between ED diagnoses. According to stepwise linear regression analyses, body mass index (BMI) and ABS-2-Awfulizing predicted greater EDI-3-ED Risk, while ABS-2-Negative Global Evaluations and GHQ predicted greater EDI-3-General Psychological Maladjustment and lower ERQ-Cognitive Reappraisal. Conclusion: Awfulizing and negative global evaluation contribute to better explaining ED-specific and non-specific ED symptoms and cognitive reappraisal. Therefore, including them, together with BMI and general psychopathology, when assessing ED patients and planning cognitive–behavioral treatment is warranted.
Direct and Indirect vSLAM Fusion for Augmented Reality
Authors: Outahar Mohamed , Moreau Guillaume , Normand Jean-Marie
Published: August 2021

Augmented reality (AR) is an emerging technology that is applied in many fields. One of the limitations that still prevents AR to be even more widely used relates to the accessibility of devices. Indeed, the devices currently used are usually high end, expensive glasses or mobile devices. vSLAM (visual simultaneous localization and mapping) algorithms circumvent this problem by requiring relatively cheap cameras for AR. vSLAM algorithms can be classified as direct or indirect methods based on the type of data used. Each class of algorithms works optimally on a type of scene (e.g., textured or untextured) but unfortunately with little overlap. In this work, a method is proposed to fuse a direct and an indirect methods in order to have a higher robustness and to offer the possibility for AR to move seamlessly between different types of scenes. Our method is tested on three datasets against state-of-the-art direct (LSD-SLAM), semi-direct (LCSD) and indirect (ORBSLAM2) algorithms in two different scenarios: a trajectory planning and an AR scenario where a virtual object is displayed on top of the video feed; furthermore, a similar method (LCSD SLAM) is also compared to our proposal. Results show that our fusion algorithm is generally as efficient as the best algorithm both in terms of trajectory (mean errors with respect to ground truth trajectory measurements) as well as in terms of quality of the augmentation (robustness and stability). In short, we can propose a fusion algorithm that, in our tests, takes the best of both the direct and indirect methods.
Microscopic Anatomy of the Lining of Hemal Spaces in the Penaeid Shrimp, Sicyonia ingentis
Authors: Sidebottom Rachel Brittany , Bang Sabi , Martin Gary
Published: August 2021

The purpose of this paper is to present a morphological description of three different types of acellular material lining hemal spaces in a shrimp, providing a background for addressing future questions. The vasculature of the penaeid shrimp, Sicyonia ingentis, includes vessels leading from the heart into arteries which branch and expand into sinuses before returning hemolymph back to the heart. Early work showed that an endothelium was absent, and a basement membrane (BM) separated tissues from the hemolymph. Therefore, it was suggested that hemocytes could identify anything other than the BM as a “foreign” entity. This study demonstrates three major types of acellular material lining the hemal spaces of S. ingentis. Cardiomyocytes, digestive gland tubules, and abdominal muscle fibers are covered by BMs. Major arteries are lined by a fibrillin-like fibrous material. Finally, sheaths of collagenous connective tissues cover the heart and digestive gland as well as the outer surface of arteries, the gut, and gonad. Our understanding of hemocyte receptors and extracellular matrices in general have greatly expanded but the biochemical composition of the matrices lining crustacean hemal spaces, their role in regulating nutrient uptake, and the cells responsible for their deposition deserve further attention.
Development of Cold Plasma Technologies for Surface Decontamination of Seed Fungal Pathogens: Present Status and Perspectives
Authors: Mravlje Jure , Regvar Marjana , Vogel-Mikuš Katarina
Published: August 2021

In view of the ever-growing human population and global environmental crisis, new technologies are emerging in all fields of our life. In the last two decades, the development of cold plasma (CP) technology has offered a promising and environmentally friendly solution for addressing global food security problems. Besides many positive effects, such as promoting seed germination, plant growth, and development, CP can also serve as a surface sterilizing agent. It can be considered a method for decontamination of microorganisms on the seed surface alternative to the traditional use of fungicides. This review covers basics of CP technology and its application in seed decontamination. As this is a relatively young field of research, the data are scarce and hard to compare due to various plasma setups and parameters. On the other hand, the rapidly growing research field offers opportunities for novel findings and applications.
TPMT*3C as a Predictor of 6-Mercaptopurine-Induced Myelotoxicity in Thai Children with Acute Lymphoblastic Leukemia
Authors: Jantararoungtong Thawinee , Wiwattanakul Supaporn , Tiyasirichokchai Rawiporn , Prommas Santirhat , Sukprasong Rattanaporn , Koomdee Napatrupron , Jinda Pimonpan , Rachanakul Jiratha , Nuntharadthanaphong Nutthan , Pakakasama Samart , Anurathapan Usanarat , Hongeng Suradej , Sukasem Chonlaphat
Published: August 2021

The response to 6-mercaptopurine (6-MP) can be altered by genetic polymorphisms in genes encoding drug-metabolizing enzymes and drug transporters. The purpose of this study was to investigate the association between genetic polymorphisms of drug-metabolizing enzymes (TPMT 719A > G (*3C), ITPA 94C > A and ITPA 123G > A) and drug transporters (MRP4 912C > A and MRP4 2269G > A) with 6-MP-related myelotoxicity and hepatotoxicity in Thai children with acute lymphoblastic leukemia (ALL). The prescribed dosage of 6-MP and its adverse effects were assessed from medical records during the first 8 weeks and 9–24 weeks of maintenance therapy. Children with the TPMT*1/*3C genotype had a higher risk of leukopenia with an odds ratio (OR) of 4.10 (95% confidence interval (CI) of 1.06–15.94; p = 0.033) compared to wild type (TPMT*1/*1) patients. Heterozygous TPMT*3C was significantly associated with severe neutropenia with an increased risk (OR, 4.17; 95% CI, 1.25–13.90); p = 0.014) during the first 8 weeks. No association was found among ITPA94C > A, ITPA123G > A, MRP4 912C > A, and MRP4 2269G > A with myelotoxicity and hepatotoxicity. The evidence that TPMT heterozygotes confer risks of 6-MP-induced myelotoxicity also supports the convincing need to genotype this pharmacogenetic marker before the initiation of 6-MP therapy.
The Effectiveness of Cobalamin (B12) Treatment for Autism Spectrum Disorder: A Systematic Review and Meta-Analysis
Authors: Rossignol Daniel A. , Frye Richard E.
Published: August 2021

Autism spectrum disorder (ASD) is a common neurodevelopmental disorder affecting 2% of children in the United States. Biochemical abnormalities associated with ASD include impaired methylation and sulphation capacities along with low glutathione (GSH) redox capacity. Potential treatments for these abnormalities include cobalamin (B12). This systematic review collates the studies using B12 as a treatment in ASD. A total of 17 studies were identified; 4 were double-blind, placebo-controlled studies (2 examined B12 injections alone and 2 used B12 in an oral multivitamin); 1 was a prospective controlled study; 6 were prospective, uncontrolled studies, and 6 were retrospective (case series and reports). Most studies (83%) used oral or injected methylcobalamin (mB12), while the remaining studies did not specify the type of B12 used. Studies using subcutaneous mB12 injections (including 2 placebo-controlled studies) used a 64.5–75 µg/kg/dose. One study reported anemia in 2 ASD children with injected cyanocobalamin that resolved with switching to injected mB12. Two studies reported improvements in markers of mitochondrial metabolism. A meta-analysis of methylation metabolites demonstrated decreased S-adenosylhomocysteine (SAH), and increased methionine, S-adenosylmethionine (SAM), SAM/SAH ratio, and homocysteine (with small effect sizes) with mB12. Meta-analysis of the transsulfuration and redox metabolism metabolites demonstrated significant improvements with mB12 in oxidized glutathione (GSSG), cysteine, total glutathione (GSH), and total GSH/GSSG redox ratio with medium to large effect sizes. Improvements in methylation capacity and GSH redox ratio were significantly associated with clinical improvements (with a mean moderate effect size of 0.59) in core and associated ASD symptoms, including expressive communication, personal and domestic daily living skills, and interpersonal, play-leisure, and coping social skills, suggesting these biomarkers may predict response to B12. Other clinical improvements observed with B12 included sleep, gastrointestinal symptoms, hyperactivity, tantrums, nonverbal intellectual quotient, vision, eye contact, echolalia, stereotypy, anemia, and nocturnal enuresis. Adverse events identified by meta-analysis included hyperactivity (11.9%), irritability (3.4%), trouble sleeping (7.6%), aggression (1.8%), and worsening behaviors (7.7%) but were generally few, mild, not serious, and not significantly different compared to placebo. In one study, 78% of parents desired to continue mB12 injections after the study conclusion. Preliminary clinical evidence suggests that B12, particularly subcutaneously injected mB12, improves metabolic abnormalities in ASD along with clinical symptoms. Further large multicenter placebo-controlled studies are needed to confirm these data. B12 is a promising treatment for ASD.
The Exudation of Surplus Products Links Plant Functional Traits and Plant-Microbial Stoichiometry
Authors: Cardenas Julian , Santa Fernando , Kaštovská Eva
Published: August 2021

The rhizosphere is a hot spot of soil microbial activity and is largely fed by root exudation. The carbon (C) exudation flux, coupled with plant growth, is considered a strategy of plants to facilitate nutrient uptake. C exudation is accompanied by a release of nutrients. Nitrogen (N) and phosphorus (P) co-limit the productivity of the plant-microbial system. Therefore, the C:N:P stoichiometry of exudates should be linked to plant nutrient economies, plant functional traits (PFT) and soil nutrient availability. We aimed to identify the strongest links in C:N:P stoichiometry among all rhizosphere components. A total of eight grass species (from conservative to exploitative) were grown in pots under two different soil C:nutrient conditions for a month. As a result, a wide gradient of plant–microbial–soil interactions were created. A total of 43 variables of plants, exudates, microbial and soil C:N:P stoichiometry, and PFTs were evaluated. The variables were merged into four groups in a network analysis, allowing us to identify the strongest connections among the variables and the biological meaning of these groups. The plant–soil interactions were shaped by soil N availability. Faster-growing plants were associated with lower amounts of mineral N (and P) in the soil solution, inducing a stronger competition for N with microorganisms in the rhizosphere compared to slower-growing plants. The plants responded by enhancing their N use efficiency and root:shoot ratio, and they reduced N losses via exudation. Root growth was supported either by reallocated foliar reserves or by enhanced ammonium uptake, which connected the specific leaf area (SLA) to the mineral N availability in the soil. Rapid plant growth enhanced the exudation flux. The exudates were rich in C and P relative to N compounds and served to release surplus metabolic products. The exudate C:N:P stoichiometry and soil N availability combined to shape the microbial stoichiometry, and N and P mining. In conclusion, the exudate flux and its C:N:P stoichiometry reflected the plant growth rate and nutrient constraints with a high degree of reliability. Furthermore, it mediated the plant–microbial interactions in the rhizosphere.
Characterization of ACE Inhibitors and AT1R Antagonists with Regard to Their Effect on ACE2 Expression and Infection with SARS-CoV-2 Using a Caco-2 Cell Model
Authors: Reus Philipp , Schneider Ann-Kathrin , Ulshöfer Thomas , Henke Marina , Bojkova Denisa , Cinatl Jindrich , Ciesek Sandra , Geisslinger Gerd , Laux Volker , Grättinger Mira , Gribbon Philip , Schiffmann Susanne
Published: August 2021

Blood-pressure-lowering drugs are proposed to foster SARS-CoV-2 infection by pharmacological upregulation of angiotensin-converting enzyme 2 (ACE2), the binding partner of the virus spike (S) protein, located on the surface of the host cells. Conversely, it is postulated that angiotensin–renin system antagonists may prevent lung damage caused by SARS-CoV-2 infection, by reducing angiotensin II levels, which can induce permeability of lung endothelial barrier via its interaction with the AT1 receptor (AT1R). Methods: We have investigated the influence of the ACE inhibitors (lisinopril, captopril) and the AT1 antagonists (telmisartan, olmesartan) on the level of ACE2 mRNA and protein expression as well as their influence on the cytopathic effect of SARS-CoV-2 and on the cell barrier integrity in a Caco-2 cell model. Results: The drugs revealed no effect on ACE2 mRNA and protein expression. ACE inhibitors and AT1R antagonist olmesartan did not influence the infection rate of SARS-CoV-2 and were unable to prevent the SARS-CoV-2-induced cell barrier disturbance. A concentration of 25 µg/mL telmisartan significantly reduced the virus replication rate. Conclusion: ACE inhibitors and AT1R antagonist showed neither beneficial nor detrimental effects on SARS-CoV-2-infection and cell barrier integrity in vitro at pharmacologically relevant concentrations.
Novel Therapies for Tongue Squamous Cell Carcinoma Patients with High-Grade Tumors
Authors: Li Yinghua , Lin Hao , Chen Lu , Chen Zihao , Li Weizhong
Published: August 2021

Background: Tongue squamous cell carcinoma (TSCC) patients with high-grade tumors usually suffer from high occurrence and poor prognosis. The current study aimed at finding the biomarkers related to tumor grades and proposing potential therapies by these biomarkers. Methods: The mRNA expression matrix of TSCC samples from The Cancer Genome Atlas (TCGA) database was analyzed to identify hub proteins related to tumor grades. The mRNA expression patterns of these hub proteins between TSCC and adjacent control samples were validated in three independent TSCC data sets (i.e., GSE9844, GSE30784, and GSE13601). The correlation between cell cycle index and immunotherapy efficacy was tested on the IMvigor210 data set. Based on the structure of hub proteins, virtual screening was applied to compounds to find the potential inhibitors. Results: A total of six cell cycle biomarkers (i.e., BUB1, CCNB2, CDC6, CDC20, CDK1, and MCM2) were selected as hub proteins by protein–protein interaction (PPI) analysis. In the validation data sets, the mRNA expression levels of these hub proteins were higher in tumor samples versus normal controls. The cell cycle index was constructed by the mRNA expression levels of these hub proteins, and patients with a high cell cycle index demonstrated favorable drug response to the immunotherapy. Three small molecules (i.e., ZINC100052685, ZINC8214703, and ZINC85537014) were found to bind with hub proteins and selected as drug candidates. Conclusion: The cell cycle index might provide a novel reference for selecting appropriate cancer patient candidates for immunotherapy. The current research might contribute to the development of precision medicine and improve the prognosis of TSCC.
Structure, Activity and Function of the SETDB1 Protein Methyltransferase
Authors: Markouli Mariam , Strepkos Dimitrios , Piperi Christina
Published: August 2021

The SET Domain Bifurcated Histone Lysine Methyltransferase 1 (SETDB1) is a prominent member of the Suppressor of Variegation 3–9 (SUV39)-related protein lysine methyltransferases (PKMTs), comprising three isoforms that differ in length and domain composition. SETDB1 is widely expressed in human tissues, methylating Histone 3 lysine 9 (H3K9) residues, promoting chromatin compaction and exerting negative regulation on gene expression. SETDB1 has a central role in normal physiology and nervous system development, having been implicated in the regulation of cell cycle progression, inactivation of the X chromosome, immune cells function, expression of retroelements and formation of promyelocytic leukemia (PML) nuclear bodies (NB). SETDB1 has been frequently deregulated in carcinogenesis, being implicated in the pathogenesis of gliomas, melanomas, as well as in lung, breast, gastrointestinal and ovarian tumors, where it mainly exerts an oncogenic role. Aberrant activity of SETDB1 has also been implicated in several neuropsychiatric, cardiovascular and gastrointestinal diseases, including schizophrenia, Huntington’s disease, congenital heart defects and inflammatory bowel disease. Herein, we provide an update on the unique structural and biochemical features of SETDB1 that contribute to its regulation, as well as its molecular and cellular impact in normal physiology and disease with potential therapeutic options.
Introducing an Intelligent Goods Service Framework
Authors: Jevinger Åse , Olsson Carl Magnus
Published: August 2021

With the increasing diffusion of Internet of Things (IoT) technologies, the transportation of goods sector is in a position to adopt novel intelligent services that cut across the otherwise highly fragmented and heterogeneous market, which today consists of a myriad of actors. Legacy systems that rely upon direct integration between all actors involved in the transportation ecosystem face considerable challenges for information sharing. Meanwhile, IoT based services, which are designed as devices that follow goods and communicate directly to cloud-based backend systems, may provide services that previously were not available. For the purposes of this paper, we present a theoretical framework for classification of such intelligent goods systems based on a literature study. The framework, labelled as the Intelligent Goods Service (IGS) framework, aims at increasing the understanding of the actors, agents, and services involved in an intelligent goods system, and to facilitate system comparisons and the development of new innovative solutions. As an illustration of how the IGS framework can be used and contribute to research in this area, we provide an example from a direct industry-academia collaboration.
Microstructure and Properties of Electroless Ni-P/Si3N4 Nanocomposite Coatings Deposited on the AW-7075 Aluminum Alloy
Authors: Czapczyk Kazimierz , Zawadzki Paweł , Wierzbicka Natalia , Talar Rafał
Published: August 2021

The article presents the results of mechanical and tribological tests of Ni-P/Si3N4 nanocomposite coatings deposited on the AW-7075 aluminum alloy using the chemical reduction method. The influence of the chemical composition on the Vickers microhardness determined by the DSI method was examined. The nanocomposite layers were made of Si3N4 silicon nitride in a polydisperse powder with a particle size ranging from 20 to 25 nm. The influence of the content of the dispersion layer material on the adhesion to the substrate was analyzed. The abrasive wear was tested and determined in the reciprocating motion using the “ball-on-flat” method. The surface topography was examined by the contact method with the use of a profilometer. Based on the obtained test results, it was found that the Ni-P/Si3N4 layers produced in the bath with the Si3N4 nanoparticle content in the amount of 2 g/dm3 are more resistant to wear and show greater adhesion than the Ni-P/Si3N4 layers deposited in the bath with 5 g/dm3 of the dispersion phase. NiP/Si3N4 layers provide protection against abrasive wear under various loads and environmental conditions.
A Study on the Mechanical Characteristics of Glass and Nylon Fiber Reinforced Peach Shell Lightweight Concrete
Authors: Ahmad Jawad , Zaid Osama , Aslam Fahid , Shahzaib Muhammad , Ullah Rahat , Alabduljabbar Hisham , Khedher Khaled Mohamed
Published: August 2021

In the current study, the utilization of glass and nylon fibers in various percentages are added to enhance the mechanical performance of peach shell lightweight concrete. Glass and nylon fibers were added at 2%, 4%, 6%, and 8% by cement weight. The results showed that, as we added the glass and nylon fibers, the density of peach shell concrete was reduced by 6.6%, and the compressive, split tensile and flexural strength were enhanced by 10.20%, 60.1%, and 63.49%. The highest strength that was obtained in compressive, split tensile, and flexural strength at 56 days was 29.4 MPa, 5.2 MPa, and 6.3 MPa, respectively, with 6% of glass fiber in peach shell concrete. Mechanical test results showed that post-failure toughness and modulus of elasticity of peach shell concrete is enhanced with the utilization of fibers. To verify our lab results, a statistical analysis, such as response surface methodology, was performed to make a statistical model, it was confirmed by both lab results and statistical analysis that the mechanical performance of peach shell concrete could be significantly improved by adding glass fibers as compared to nylon fibers. With the use of fibers, the water absorption and porosity were slightly increased. Hence, the glass and nylon fibers can be used to improve the peach shell concrete mechanical properties to make concrete eco-friendly, sustainable, and lightweight.
Recent Developments on MnN for Spintronic Applications
Authors: Vallejo-Fernandez Gonzalo , Meinert Markus
Published: August 2021

There is significant interest worldwide to identify new antiferromagnetic materials suitable for device applications. Key requirements for such materials are: relatively high magnetocrystalline anisotropy constant, low cost, high corrosion resistance and the ability to induce a large exchange bias, i.e., loop shift, when grown adjacent to a ferromagnetic layer. In this article, a review of recent developments on the novel antiferromagnetic material MnN is presented. This material shows potential as a replacement for the commonly used antiferromagnet of choice, i.e., IrMn. Although the results so far look promising, further work is required for the optimization of this material.