bluuebunny/crossref_metadata_2025 huggingface dataset:
The Attorney-General, politics and logistics – a fork in the road?
Authors: Hand James
Published: January 2022

The appointment of recent Attorney-Generals for England and Wales has occasioned much comment about their experience. This paper considers whether, following the transmogrification of the Lord Chancellorship over a decade ago, the backgrounds and activities of recent leaders of the Attorney-General's Office suggest the time is now right for similar changes to the Law Officers’ roles. In doing so, it presents a range of original data on aspects of the role and on characteristics of Attorney-Generals, which suggests that unless self-restraint is exercised (by the Prime Minister and the post-holders) we may ineluctably be on the path to reform.
Childhood adversities and mental disorders in first-year college students: results from the World Mental Health International College Student Initiative
Authors: Husky Mathilde M. , Sadikova Ekaterina , Lee Sue , Alonso Jordi , Auerbach Randy P. , Bantjes Jason , Bruffaerts Ronny , Cuijpers Pim , Ebert David D. , Garcia Raùl Gutiérrez , Hasking Penelope , Mak Arthur , McLafferty Margaret , Sampson Nancy A. , Stein Dan J. , Kessler Ronald C. ,   WHO WMH-ICS collaborators
Published: January 2022

Background

This study investigates associations of several dimensions of childhood adversities (CAs) with lifetime mental disorders, 12-month disorder persistence, and impairment among incoming college students.

Methods

Data come from the World Mental Health International College Student Initiative (WMH-ICS). Web-based surveys conducted in nine countries (n = 20 427) assessed lifetime and 12-month mental disorders, 12-month role impairment, and seven types of CAs occurring before the age of 18: parental psychopathology, emotional, physical, and sexual abuse, neglect, bullying victimization, and dating violence. Poisson regressions estimated associations using three dimensions of CA exposure: type, number, and frequency.

Results

Overall, 75.8% of students reported exposure to at least one CA. In multivariate regression models, lifetime onset and 12-month mood, anxiety, and substance use disorders were all associated with either the type, number, or frequency of CAs. In contrast, none of these associations was significant when predicting disorder persistence. Of the three CA dimensions examined, only frequency was associated with severe role impairment among students with 12-month disorders. Population-attributable risk simulations suggest that 18.7–57.5% of 12-month disorders and 16.3% of severe role impairment among those with disorders were associated with these CAs.

Conclusion

CAs are associated with an elevated risk of onset and impairment among 12-month cases of diverse mental disorders but are not involved in disorder persistence. Future research on the associations of CAs with psychopathology should include fine-grained assessments of CA exposure and attempt to trace out modifiable intervention targets linked to mechanisms of associations with lifetime psychopathology and burden of 12-month mental disorders.
Efficacy of cognitive therapy and behavior therapy for menopausal symptoms: a systematic review and meta-analysis
Authors: Ye Mengfei , Shou Mengna , Zhang Jian , Hu Baiqi , Liu Chunyan , Bi Chenchen , Lv Tingting , Luo Fangyi , Zhang Zeying , Liang Shenglin , Feng Hong , Qian Chao , Cao Shilin , Liu Zheng
Published: January 2022

Background

T long-term effects of cognitive therapy and behavior therapy (CTBT) for menopausal symptoms are unknown, and whether the effects are different between natural menopause and treatment-induced menopause are currently unclear. Therefore, we sought to conduct an accurate estimate of the efficacy of CTBT for menopausal symptoms.

Methods

We conducted searches of Cochrane Library, EMBASE, PsycINFO, PubMed, and Web of Science databases for studies from 1 January 1977 to 1 November 2021. Randomized controlled trials (RCTs) comparing intervention groups to control groups for menopausal symptoms were included. Hedge's g was used as the standardized between-group effect size with a random-effects model.

Results

We included 14 RCTs comprising 1618 patients with a mean sample size of 116. CTBT significantly outperformed control groups in terms of reducing hot flushes [g = 0.39, 95% confidence interval (CI) 0.23–0.55, I2 = 45], night sweats, depression (g = 0.50, 95% CI 0.34–0.66, I2 = 51), anxiety (g = 0.38, 95% CI 0.23–0.54, I2 = 49), fatigue, and quality of life. Egger's test indicated no publication bias.

Conclusions

CTBT is an effective psychological treatment for menopausal symptoms, with predominantly small to moderate effects. The efficacy is sustained long-term, although it declines somewhat over time. The efficacy was stronger for natural menopause symptoms, such as vasomotor symptoms, than for treatment-induced menopause symptoms. These findings provide support for treatment guidelines recommending CTBT as a treatment option for menopausal symptoms.
Olfactory reference plus Truman symptoms in one patient with Gilbert syndrome and antiphospholipid antibodies (Hughes disease) secondary to probable chronic Lyme neuroborreliosis
Authors: Almeida e Melo João , Gama Marques João
Published: January 2022

After reading an article in the journal, regarding affective disorders in patients with rare illnesses, the authors would like to discuss a case of non-affective psychosis, presenting with olfactory reference and Truman symptoms, in a patient with three unusual conditions: Gilbert disease, Hughes syndrome and Lyme neuroborreliosis.
Subgraph densities in a surface
Authors: Huynh Tony , Joret Gwenaël , Wood David R.
Published: January 2022

Given a fixed graphHthat embeds in a surface$\Sigma$, what is the maximum number of copies ofHin ann-vertex graphGthat embeds in$\Sigma$? We show that the answer is$\Theta(n^{f(H)})$, wheref(H) is a graph invariant called the ‘flap-number’ ofH, which is independent of$\Sigma$. This simultaneously answers two open problems posed by Eppstein ((1993)J. Graph Theory17(3) 409–416.). The same proof also answers the question for minor-closed classes. That is, ifHis a$K_{3,t}$minor-free graph, then the maximum number of copies ofHin ann-vertex$K_{3,t}$minor-free graph G is$\Theta(n^{f'(H)})$, wheref′(H) is a graph invariant closely related to the flap-number ofH. Finally, whenHis a complete graph we give more precise answers.
Musicking and DancingImbaluat Namasho: Enacting Indigenous Education Among the Bagisu, Uganda
Authors: MAKWA DOMINIC D.B.
Published: January 2022

This paper discusses how the activities during which people gather to create, perform, and transmitimbalucircumcision music and dance at Namasho Cultural Site (NCS) become a platform for the Bagisu of the Balutseshe clan to tutor boys about society’s gender ideology, social histories, and rituals of the sacred swamp, thus turning the place into a communal classroom for imparting this knowledge. Due to the changing context of contemporary Bagisu, I argue for efforts to archive these events to make them accessible by future generations in order to upholdimbaluperformances as a mechanism of knowledge production and dissemination.
“That is Why I am Telling this Story”: Musical Analysis as Insight into the Transmission of Knowledge and Performance Practice of a Wapurtarli Song by Warlpiri Women from Yuendumu, Central Australia
Authors: CURRAN GEORGIA , YEOH CALISTA
Published: January 2022

Insights into the knowledge, performance, and transmission of songs are pivotal in ensuring the survival of traditional Aboriginal songs. We present the first in-depth musical analysis of a Wapurtarli yawulyu song set sung by Warlpiri women from Yuendumu, Central Australia, recorded in December 2006 with a solo lead singer accompanied by a small group. Our musical analysis reveals that there are various interlocking parts of a song, and this can make it difficult for current generations to learn songs. The context of musical endangerment and the musical analyses presented in our study show that contemporary spaces for learning yawulyu must consider the complex components that come together for a song set to be properly performed.
Seismic Site Response Inferred From Records at a Dense Linear Array Across the Chenghai Fault Zone, Binchuan, Yunnan
Authors: Song Junhao , Yang Hongfeng
Published: January 2022

Low‐velocity zones (LVZ) in the shallow crust such as basins infilled with unconsolidated sediments could significantly amplify seismic waves and cause intense ground motions. In this study, we estimate the seismic site response in the Binchuan basin across the active Chenghai fault zone in northwest Yunnan Province, Southwest China using data from a large‐aperture (∼8 km) dense linear array of 125 three‐component seismometers deployed in 2018 for ∼1 month. We observe that local earthquakes with larger epicentral distances tend to produce stronger spatial variability in peak ground velocity (PGV) across the array. The PGV values are overall larger in the middle of the array than the two sides, especially in the horizontal directions, which is consistent with both tomographic and traveltime results. Using both nearby and distant earthquakes, we find that horizontal ground motions from 0.2 to 2 Hz at stations inside the LVZ are up to 20 times as large as that outside, and a more localized zone (∼500 m wide) enclosing the fault surface trace is characterized by broader frequency bands and larger factors of amplification. As the distance from the fault increases, amplification factors become smaller while the lower limit of the amplification frequency band gradually increases. Variations in predicted ground motion from one‐dimensional SH‐wave modeling reveal amplification and attenuation effects of the LVZ, suggesting that strong lateral heterogeneity should be considered. Our results demonstrate that based on dense array data, analyzing seismic site response is an alternative method to effectively constrain the shallow subsurface fault structure.
Evaluating Climate Models’ Cloud Feedbacks Against Expert Judgment
Authors: Zelinka Mark D. , Klein Stephen A. , Qin Yi , Myers Timothy A.
Published: January 2022

The persistent and growing spread in effective climate sensitivity (ECS) across global climate models necessitates rigorous evaluation of their cloud feedbacks. Here we evaluate several cloud feedback components simulated in 19 climate models against benchmark values determined via an expert synthesis of observational, theoretical, and high‐resolution modeling studies. We find that models with smallest feedback errors relative to these benchmark values generally have moderate total cloud feedbacks (0.4–0.6 W m−2 K−1) and ECS (3–4 K). Those with largest errors generally have total cloud feedback and ECS values that are too large or too small. Models tend to achieve large positive total cloud feedbacks by having several cloud feedback components that are systematically biased high rather than by having a single anomalously large component, and vice versa. In general, better simulation of mean‐state cloud properties leads to stronger but not necessarily better cloud feedbacks. The Python code base provided herein could be applied to developmental versions of models to assess cloud feedbacks and cloud errors and place them in the context of other models and of expert judgment in real‐time during model development.
Floodplain Sediment Storage Timescales of the Laterally Confined Meandering Powder River, USA
Authors: Huffman Max E. , Pizzuto James E. , Trampush Sheila M. , Moody John A. , Schook Derek M. , Gray Harrison J. , Mahan Shannon A.
Published: January 2022

As sediment is transported through river corridors, it typically spends more time in storage than transport, and as a result, sediment delivery timescales are controlled by the duration of storage. Present understanding of storage timescales is largely derived from models or from field studies covering relatively short (≤102 year) time spans. Here we quantify the storage time distribution for a 17 km length of Powder River in Montana, USA by determining the age distribution of eroded sediment. Our approach integrates surveyed cross‐sections, analysis of historical aerial imagery, aerial LiDAR, geomorphic mapping, and age control provided by optically stimulated luminescence (OSL) and dendrochronology. Sediment eroded by Powder River from 1998 to 2013 ranges from a few years to ∼5,000 years in age; ages are exponentially distributed (r2 = 0.78; Anderson‐Darling p value 0.003). Eroded sediment is derived from Powder River's meander belt (∼900 m wide), which is only 1.25 times its meander wavelength, a value reflecting valley confinement rather than free meandering. The mean storage time, 824 years (95% C.I. 610–1030 years), is similar to the time required to rework deposits of Powder River's meander belt based on an average meander migration rate of ∼1 m/yr, implying that storage time distributions of confined meandering rivers can be quantified from remotely sensed estimates of meander belt width and channel migration rates. Heavy‐tailed storage time distributions, frequently cited from physical and numerical modeling studies, may be restricted to unconfined meandering rivers.
Effective Menin inhibitor-based combinations against AML with MLL rearrangement or NPM1 mutation (NPM1c)
Authors: Fiskus Warren , Boettcher Steffen , Daver Naval , Mill Christopher P. , Sasaki Koji , Birdwell Christine E. , Davis John A. , Takahashi Koichi , Kadia Tapan M. , DiNardo Courtney D. , Jin Qi , Qi Yuan , Su Xiaoping , McGeehan Gerard M. , Khoury Joseph D. , Ebert Benjamin L. , Bhalla Kapil N.
Published: January 2022

Treatment with Menin inhibitor (MI) disrupts the interaction between Menin and MLL1 or MLL1-fusion protein (FP), inhibits HOXA9/MEIS1, induces differentiation and loss of survival of AML harboring MLL1 re-arrangement (r) and FP, or expressing mutant (mt)-NPM1. Following MI treatment, although clinical responses are common, the majority of patients with AML with MLL1-r or mt-NPM1 succumb to their disease. Pre-clinical studies presented here demonstrate that genetic knockout or degradation of Menin or treatment with the MI SNDX-50469 reduces MLL1/MLL1-FP targets, associated with MI-induced differentiation and loss of viability. MI treatment also attenuates BCL2 and CDK6 levels. Co-treatment with SNDX-50469 and BCL2 inhibitor (venetoclax), or CDK6 inhibitor (abemaciclib) induces synergistic lethality in cell lines and patient-derived AML cells harboring MLL1-r or mtNPM1. Combined therapy with SNDX-5613 and venetoclax exerts superior in vivo efficacy in a cell line or PD AML cell xenografts harboring MLL1-r or mt-NPM1. Synergy with the MI-based combinations is preserved against MLL1-r AML cells expressing FLT3 mutation, also CRISPR-edited to introduce mtTP53. These findings highlight the promise of clinically testing these MI-based combinations against AML harboring MLL1-r or mtNPM1.
The antibody response of haematological malignancies to COVID-19 infection and vaccination
Authors: Seebacher Nicole A.
Published: January 2022

Cancer patients with COVID-19 have reduced survival. While most cancer patients, like the general population, have an almost 100% rate of seroconversion after COVID-19 infection or vaccination, patients with haematological malignancies have lower seroconversion rates and are far less likely to gain adequate protection. This raises the concern that patients with haematological malignancies, especially those receiving immunosuppressive therapies, may still develop the fatal disease when infected with COVID-19 after vaccination. There is an urgent need to develop Guidelines to help direct vaccination schedules and protective measures in oncology patients, differentiating those with haematological malignancies and those in an immunocompromised state.
Long noncoding RNA FAM225A promotes the malignant progression of gastric cancer through the miR-326/PADI2 axis
Authors: Ma Xiang , Wang Gang , Fan Hao , Li Zengliang , Chen Wangwang , Xiao Jian , Ni Peidong , Liu Kanghui , Shen Kuan , Wang Yuanhang , Xu Zekuan , Yang Li
Published: January 2022

Gastric cancer (GC) is a global health problem and further studies of its molecular mechanisms are needed to identify effective therapeutic targets. Although some long noncoding RNAs (lncRNAs) have been found to be involved in the progression of GC, the molecular mechanisms of many GC-related lncRNAs remain unclear. In this study, a series of in vivo and in vitro assays were performed to study the relationship between FAM225A and GC, which showed that FAM225A levels were correlated with poor prognosis in GC. Higher FAM225A expression tended to be correlated with a more profound lymphatic metastasis rate, larger tumor size, and more advanced tumor stage. FAM225A also promoted gastric cell proliferation, invasion, and migration. Further mechanistic investigation showed that FAM225A acted as a miR-326 sponge to upregulate its direct target PADI2 in GC. Overall, our findings indicated that FAM225A promoted GC development and progression via a competitive endogenous RNA network of FAM225A/miR-326/PADI2 in GC, providing insight into possible therapeutic targets and prognosis of GC.
Complementing the phenotypical spectrum of TUBA1A tubulinopathy and its role in early-onset epilepsies
Authors: Schröter Julian , Popp Bernt , Brennenstuhl Heiko , Döring Jan H. , Donze Stephany H. , Bijlsma Emilia K. , van Haeringen Arie , Huhle Dagmar , Jestaedt Leonie , Merkenschlager Andreas , Arelin Maria , Gräfe Daniel , Neuser Sonja , Oates Stephanie , Pal Deb K. , Parker Michael J. , Lemke Johannes R. , Hoffmann Georg F. , Kölker Stefan , Harting Inga , Syrbe Steffen
Published: January 2022

TUBA1A tubulinopathy is a rare neurodevelopmental disorder associated with brain malformations as well as early-onset and intractable epilepsy. As pathomechanisms and genotype-phenotype correlations are not completely understood, we aimed to provide further insights into the phenotypic and genetic spectrum. We here present a multicenter case series of ten unrelated individuals from four European countries using systematic MRI re-evaluation, protein structure analysis, and prediction score modeling. In two cases, pregnancy was terminated due to brain malformations. Amongst the eight living individuals, the phenotypic range showed various severity. Global developmental delay and severe motor impairment with tetraparesis was present in 63% and 50% of the subjects, respectively. Epilepsy was observed in 75% of the cases, which showed infantile onset in 83% and a refractory course in 50%. One individual presented a novel TUBA1A-associated electroclinical phenotype with evolvement from early myoclonic encephalopathy to continuous spike-and-wave during sleep. Neuroradiological features comprised a heterogeneous spectrum of cortical and extracortical malformations including rare findings such as cobblestone lissencephaly and subcortical band heterotopia. Two individuals developed hydrocephalus with subsequent posterior infarction. We report four novel and five previously published TUBA1A missense variants whose resulting amino acid substitutions likely affect longitudinal, lateral, and motor protein interactions as well as GTP binding. Assessment of pathogenic and benign variant distributions in synopsis with prediction scores revealed sections of variant enrichment and intolerance to missense variation. We here extend the clinical, neuroradiological, and genetic spectrum of TUBA1A tubulinopathy and provide insights into residue-specific pathomechanisms and genotype-phenotype correlations.
Physical geography, isolation by distance and environmental variables shape genomic variation of wild barley (Hordeum vulgare L. ssp. spontaneum) in the Southern Levant
Authors: Chang Che-Wei , Fridman Eyal , Mascher Martin , Himmelbach Axel , Schmid Karl
Published: January 2022

Determining the extent of genetic variation that reflects local adaptation in crop-wild relatives is of interest for the purpose of identifying useful genetic diversity for plant breeding. We investigated the association of genomic variation with geographical and environmental factors in wild barley (Hordeum vulgare L. ssp. spontaneum) populations of the Southern Levant using genotyping by sequencing (GBS) of 244 accessions in the Barley 1K+ collection. The inference of population structure resulted in four genetic clusters that corresponded to eco-geographical habitats and a significant association between lower gene flow rates and geographical barriers, e.g. the Judaean Mountains and the Sea of Galilee. Redundancy analysis (RDA) revealed that spatial autocorrelation explained 45% and environmental variables explained 15% of total genomic variation. Only 4.5% of genomic variation was solely attributed to environmental variation if the component confounded with spatial autocorrelation was excluded. A synthetic environmental variable combining latitude, solar radiation, and accumulated precipitation explained the highest proportion of genomic variation (3.9%). When conditioned on population structure, soil water capacity was the most important environmental variable explaining 1.18% of genomic variation. Genome scans with outlier analysis and genome-environment association studies were conducted to identify adaptation signatures. RDA and outlier methods jointly detected selection signatures in the pericentromeric regions, which have reduced recombination, of the chromosomes 3H, 4H, and 5H. However, selection signatures mostly disappeared after correction for population structure. In conclusion, adaptation to the highly diverse environments of the Southern Levant over short geographical ranges had a limited effect on the genomic diversity of wild barley. This highlighted the importance of nonselective forces in genetic differentiation.
Ensemble streamflow forecasting based on variational mode decomposition and long short term memory
Authors: Sun Xiaomei , Zhang Haiou , Wang Jian , Shi Chendi , Hua Dongwen , Li Juan
Published: January 2022

Reliable and accurate streamflow forecasting plays a vital role in the optimal management of water resources. To improve the stability and accuracy of streamflow forecasting, a hybrid decomposition-ensemble model named VMD-LSTM-GBRT, which is sensitive to sampling, noise and long historical changes of streamflow, was established. The variational mode decomposition (VMD) algorithm was first applied to extract features, which were then learned by several long short-term memory (LSTM) networks. Simultaneously, an ensemble tree, a gradient boosting tree for regression (GBRT), was trained to model the relationships between the extracted features and the original streamflow. The outputs of these LSTMs were finally reconstructed by the GBRT model to obtain the forecasting streamflow results. A historical daily streamflow series (from 1/1/1997 to 31/12/2014) for Yangxian station, Han River, China, was investigated by the proposed model. VMD-LSTM-GBRT was compared with respect to three aspects: (1) feature extraction algorithm; ensemble empirical mode decomposition (EEMD) was used. (2) Feature learning techniques; deep neural networks (DNNs) and support vector machines for regression (SVRs) were exploited. (3) Ensemble strategy; the summation strategy was used. The results indicate that the VMD-LSTM-GBRT model overwhelms all other peer models in terms of the root mean square error (RMSE = 36.3692), determination coefficient (R2 = 0.9890), mean absolute error (MAE = 9.5246) and peak percentage threshold statistics (PPTS(5) = 0.0391%). The addressed approach based on the memory of long historical changes with deep feature representations had good stability and high prediction precision.
The Amazon River plume, a barrier to animal dispersal in the Western Tropical Atlantic
Authors: Giachini Tosetto Everton , Bertrand Arnaud , Neumann-Leitão Sigrid , Nogueira Júnior Miodeli
Published: January 2022

The dispersal of marine organisms can be restricted by a set of isolation mechanisms including hard barriers or hydrological features. In the Western Atlantic Ocean, the Amazon River discharge has been shown to act as a biogeographical barrier responsible for the differences in reef fish communities between Caribbean Sea and Northeast Brazil continental shelves. Here, we compare the diversity of all Animalia phyla from biogeographic ecoregions along the Tropical Western Atlantic continental shelf to test the hypothesis that the Amazon River plume spatially structures species diversity. For that, we used beta diversity estimators and multivariate ecological analysis on a database of species occurrence of the whole animal kingdom including 175,477 occurrences of 8,375 species from six ecoregions along the Western Tropical Atlantic. Results of the whole animal kingdom and the richest phyla showed that the Caribbean Sea and Tropical Brazil ecoregions are isolated by the Amazon River Plume, broadening and confirming the hypothesis that it acts as a soft barrier to animal dispersal in the Western Tropical Atlantic. Species sharing is larger northwestwards, in direction of the Caribbean than the opposite direction. Beyond species isolation due to local characteristics such as low salinity and high turbidity, our results suggest the dominant northwestward currents probably play a major role in animal dispersion: it enhances the flux of larvae and other planktonic organisms with reduced mobility from Brazil to Caribbean and hinders their contrary movement. Thus, the Amazon area is a strong barrier for taxa with reduced dispersal capacity, while species of pelagic taxa with active swimming may transpose it more easily.
Hybrid systems using residual modeling for sea surface temperature forecasting
Authors: de Mattos Neto Paulo S. G. , Cavalcanti George D. C. , de O. Santos Júnior Domingos S. , Silva Eraylson G.
Published: January 2022

The sea surface temperature (SST) is an environmental indicator closely related to climate, weather, and atmospheric events worldwide. Its forecasting is essential for supporting the decision of governments and environmental organizations. Literature has shown that single machine learning (ML) models are generally more accurate than traditional statistical models for SST time series modeling. However, the parameters tuning of these ML models is a challenging task, mainly when complex phenomena, such as SST forecasting, are addressed. Issues related to misspecification, overfitting, or underfitting of the ML models can lead to underperforming forecasts. This work proposes using hybrid systems (HS) that combine (ML) models using residual forecasting as an alternative to enhance the performance of SST forecasting. In this context, two types of combinations are evaluated using two ML models: support vector regression (SVR) and long short-term memory (LSTM). The experimental evaluation was performed on three datasets from different regions of the Atlantic Ocean using three well-known measures: mean square error (MSE), mean absolute percentage error (MAPE), and mean absolute error (MAE). The best HS based on SVR improved the MSE value for each analyzed series by $$82.26\%$$

82.26
%

, $$98.93\%$$

98.93
%

, and $$65.03\%$$

65.03
%

compared to its respective single model. The HS employing the LSTM improved $$92.15\%$$

92.15
%

, $$98.69\%$$

98.69
%

, and $$32.41\%$$

32.41
%

concerning the single LSTM model. Compared to literature approaches, at least one version of HS attained higher accuracy than statistical and ML models in all study cases. In particular, the nonlinear combination of the ML models obtained the best performance among the proposed HS versions.
The incidence of COVID-19 infection following emergency use authorization of BBIBP-CORV inactivated vaccine in frontline workers in the United Arab Emirates
Authors: Al Kaabi Nawal , Oulhaj Abderrahim , Al Hosani Farida Ismail , Al Mazrouei Shamma , Najim Omer , Hussein Salah Eldin , Abdalla Jehad Saleh , Fasihuddin Mohammed Saifuddin , Hassan Afnan Abdellatif , Elghazali Gehad , Al Rumaithi Ahmed , Al Azazi Jumana , Weber Stefan , Beiram Rami , Parekh Khatija A. , Sheek-Hussein Mohamud , Yang Yunkai , Xiaoming Yang , Quliang Jenny , Eltantawy Islam , Mahmoud Sally , Koshy Ashish , Xiao Peng , Ganesan Subhashini , Elamin Wael , Zaher Walid
Published: January 2022

Based on the findings from the Phase III clinical trials of inactivated SARS COV-2 Vaccine, (BBIBP-CORV) emergency use authorization (EUA) was granted for the vaccine to frontline workers in the UAE. A prospective cohort study was conducted among frontline workers to estimate the incidence rate and risk of symptomatic COVID-19 infection 14 days after the second dose of inoculation with BBIBP-CORV inactivated vaccine. Those who received two doses of the BBIBP-CORV vaccine in the period from 14th of September 2020 (first dose) to 21st of December 2020 (second dose) were followed up for COVID-19 infections. 11,322 individuals who received the two-dose BBIBP-CORV vaccine were included and were followed up post the second dose plus fourteen days. The incidence rate of symptomatic infection was 0.08 per 1000-person days (95% CI 0.07, 0.10). The estimated absolute risk of developing symptomatic infection was 0.97% (95% CI 0.77%, 1.17%). The confirmed seroconversion rate was 92.8%. There were no serious adverse events reported and no individuals suffered from severe disease. Our findings show that vaccinated individuals are likely to remain protected against symptomatic infection or becoming PCR positive for SARS COV 2 following the second dose of the vaccination.
Rapid video-based deep learning of cognate versus non-cognate T cell-dendritic cell interactions
Authors: Anandakumaran Priya N. , Ayers Abigail G. , Muranski Pawel , Creusot Remi J. , Sia Samuel K.
Published: January 2022

Identification of cognate interactions between antigen-specific T cells and dendritic cells (DCs) is essential to understanding immunity and tolerance, and for developing therapies for cancer and autoimmune diseases. Conventional techniques for selecting antigen-specific T cells are time-consuming and limited to pre-defined antigenic peptide sequences. Here, we demonstrate the ability to use deep learning to rapidly classify videos of antigen-specific CD8+ T cells. The trained model distinguishes distinct interaction dynamics (in motility and morphology) between cognate and non-cognate T cells and DCs over 20 to 80 min. The model classified high affinity antigen-specific CD8+ T cells from OT-I mice with an area under the curve (AUC) of 0.91, and generalized well to other types of high and low affinity CD8+ T cells. The classification accuracy achieved by the model was consistently higher than simple image analysis techniques, and conventional metrics used to differentiate between cognate and non-cognate T cells, such as speed. Also, we demonstrated that experimental addition of anti-CD40 antibodies improved model prediction. Overall, this method demonstrates the potential of video-based deep learning to rapidly classify cognate T cell-DC interactions, which may also be potentially integrated into high-throughput methods for selecting antigen-specific T cells in the future.
Effects of insertion torque values on the marginal bone loss of dental implants installed in sheep mandibles
Authors: Gehrke Sergio Alexandre , Júnior Jaime Aramburú , Treichel Tiago Luis Eirles , do Prado Tales Dias , Dedavid Berenice Anina , de Aza Piedad N.
Published: January 2022

The aim of the present in vivo study was to analyze and compare the effects on the crestal bone healing of two different implant macrogeometries installed in fresh socket areas and in normal bone areas with different insertion torque values. Two implant macrogeometries were used in the present study, DuoCone implant (DC) and Maestro implant (MAE), forming four groups: group DCws, in which the implants were installed in healing bone (without a socket); group DCfs, in which the implants were installed in post-extraction areas (fresh sockets); group MAEws, in which the implants were installed in healing bone (without a socket); group MAEfs, in which the implants were installed in post-extraction areas (fresh sockets). After 30 and 90 days of implantations in the bilateral mandibles of 10 sheep, eighty implants were evaluated through digital X-ray images and histologic slices. The crestal bone position in relation to the implant platform shoulder was measured and compared. The measured insertion torque was 47.2 ± 4.69 Ncm for the DCws group, 43.4 ± 4.87 Ncm for the DCfs group, 29.3 ± 3.16 Ncm for the MAEws group, and 27.7 ± 4.41 Ncm for the MAEfs group. The radiographic mesio-distal and histological bucco-lingual analyses showed significantly greater vertical bone loss in the implants installed with high torque (DC groups) in comparison to the implants installed with a low torque (MAE groups) (p < 0.05), at both evaluation times. In general, low insertion torque values (Maestro implants) showed better results of MBL when compared to implants installed with higher torque values (Duo Cone implants). Moreover, our results showed that the implants installed in the sites without sockets showed a less MBL in comparison with the implants installed in sites of fresh sockets.
Investigating the impact of emotion on temporal orientation in a deep multitask setting
Authors: Kamila Sabyasachi , Hasanuzzaman Mohammad , Ekbal Asif , Bhattacharyya Pushpak
Published: January 2022

Temporal orientation is an important aspect of human cognition which shows how an individual emphasizes past, present, and future. Theoretical research in psychology shows that one’s emotional state can influence his/her temporal orientation. We hypothesize that measuring human temporal orientation can benefit from concurrent learning of emotion. To test this hypothesis, we propose a deep learning-based multi-task framework where we concurrently learn a unified model for temporal orientation (our primary task) and emotion analysis (secondary task) using tweets. Our multi-task framework takes users’ tweets as input and produces three temporal orientation labels (past, present or future) and four emotion labels (joy, sadness, anger, or fear) with intensity values as outputs. The classified tweets are then grouped for each user to obtain the user-level temporal orientation and emotion. Finally, we investigate the associations between the users’ temporal orientation and their emotional state. Our analysis reveals that joy and anger are correlated to future orientation while sadness and fear are correlated to the past orientation.
Transcriptional alterations in bladder epithelial cells in response to infection with different morphological states of uropathogenic Escherichia coli
Authors: Persson Katarina , Petersson Ulrika , Johansson Charlotte , Demirel Isak , Kruse Robert
Published: January 2022

Uropathogenic Escherichia coli (UPEC) may undergo a cyclic cascade of morphological alterations that are believed to enhance the potential of UPEC to evade host responses and re-infect host cell. However, knowledge on the pathogenic potential and host activation properties of UPEC during the morphological switch is limited. Microarray analysis was performed on mRNA isolated from human bladder epithelial cells (HBEP) after exposure to three different morphological states of UPEC (normal coliform, filamentous form and reverted form). Cells stimulated with filamentous bacteria showed the lowest number of significant gene alterations, although the number of enriched gene ontology classes was high suggesting diverse effects on many different classes of host genes. The normal coliform was in general superior in stimulating transcriptional activity in HBEP cells compared to the filamentous and reverted form. Top-scored gene entities activated by all three morphological states included IL17C, TNFAIP6, TNF, IL20, CXCL2, CXCL3, IL6 and CXCL8. The number of significantly changed canonical pathways was lower in HBEP cells stimulated with the reverted form (32 pathways), than in cells stimulated with the coliform (83 pathways) or filamentous bacteria (138 pathways). A host cell invasion assay showed that filamentous bacteria were unable to invade bladder cells, and that the number of intracellular bacteria was markedly lower in cells infected with the reverted form compared to the coliform. In conclusion, the morphological state of UPEC has major impact on the host bladder response both when evaluating the number and the identity of altered host genes and pathways.
KIBRA, MTNR1B, and FKBP5 genotypes are associated with decreased odds of incident delirium in elderly post-surgical patients
Authors: Terrelonge Mark , LaHue Sara C. , Tang Christopher , Movsesyan Irina , Pullinger Clive R. , Dubal Dena B. , Leung Jacqueline , Douglas Vanja C.
Published: January 2022

Despite the association between cognitive impairment and delirium, little is known about whether genetic differences that confer cognitive resilience also confer resistance to delirium. To investigate whether older adults without postoperative delirium, compared with those with postoperative delirium, are more likely to have specific single nucleotide polymorphisms (SNPs) in the FKBP5, KIBRA, KLOTHO, MTNR1B, and SIRT1 genes known to be associated with cognition or delirium. This prospective nested matched exploratory case–control study included 94 older adults who underwent orthopedic surgery and screened for postoperative delirium. Forty-seven subjects had incident delirium, and 47 age-matched controls were not delirious. The primary study outcome was genotype frequency for the five SNPs. Compared with participants with delirium, those without delirium had higher adjusted odds of KIBRA SNP rs17070145 CT/TT [vs. CC; adjusted odds ratio (aOR) 2.80, 95% confidence interval (CI) 1.03, 7.54; p = 0.04] and MTNR1B SNP rs10830963 CG/GG (vs. CC; aOR 4.14, 95% CI 1.36, 12.59; p = 0.01). FKBP5 SNP rs1360780 CT/TT (vs. CC) demonstrated borderline increased adjusted odds of not developing delirium (aOR 2.51, 95% CI 1.00, 7.34; p = 0.05). Our results highlight the relevance of KIBRA, MTNR1B, and FKBP5 in understanding the complex relationship between delirium, cognition, and sleep, which warrant further study in larger, more diverse populations.
3D nanomechanical mapping of subcellular and sub-nuclear structures of living cells by multi-harmonic AFM with long-tip microcantilevers
Authors: Efremov Yuri M. , Suter Daniel M. , Timashev Peter S. , Raman Arvind
Published: January 2022

Recent developments such as multi-harmonic Atomic Force Microscopy (AFM) techniques have enabled fast, quantitative mapping of nanomechanical properties of living cells. Due to their high spatiotemporal resolution, these methods provide new insights into changes of mechanical properties of subcellular structures due to disease or drug response. Here, we propose three new improvements to significantly improve the resolution, identification, and mechanical property quantification of sub-cellular and sub-nuclear structures using multi-harmonic AFM on living cells. First, microcantilever tips are streamlined using long-carbon tips to minimize long-range hydrodynamic interactions with the cell surface, to enhance the spatial resolution of nanomechanical maps and minimize hydrodynamic artifacts. Second, simultaneous Spinning Disk Confocal Microscopy (SDC) with live-cell fluorescent markers enables the unambiguous correlation between observed heterogeneities in nanomechanical maps with subcellular structures. Third, computational approaches are then used to estimate the mechanical properties of sub-nuclear structures. Results are demonstrated on living NIH 3T3 fibroblasts and breast cancer MDA-MB-231 cells, where properties of nucleoli, a deep intracellular structure, were assessed. The integrated approach opens the door to study the mechanobiology of sub-cellular structures during disease or drug response.
Genetic diversity and mate selection in a reintroduced population of gray wolves
Authors: Ausband David E.
Published: January 2022

The genetic composition of an individual can markedly affect its survival, reproduction, and ultimately fitness. As some wildlife populations become smaller, conserving genetic diversity will be a conservation challenge. Many imperiled species are already supported through population augmentation efforts and we often do not know if or how genetic diversity is maintained in translocated species. As a case study for understanding the maintenance of genetic diversity in augmented populations, I wanted to know if genetic diversity (i.e., observed heterozygosity) remained high in a population of gray wolves in the Rocky Mountains of the U.S. > 20 years after reintroduction. Additionally, I wanted to know if a potential mechanism for such diversity was individuals with below average genetic diversity choosing mates with above average diversity. I also asked whether there was a preference for mating with unrelated individuals. Finally, I hypothesized that mated pairs with above average heterozygosity would have increased survival of young. Ultimately, I found that females with below average heterozygosity did not choose mates with above average heterozygosity and wolves chose mates randomly with respect to genetic relatedness. Pup survival was not higher for mated pairs with above average heterozygosity in my models. The dominant variables predicting pup survival were harvest rate during their first year of life and years pairs were mated. Ultimately, genetic diversity was relatively unchanged > 20 years after reintroduction. The mechanism for maintaining such diversity does not appear related to individuals preferentially choosing more genetically diverse mates. Inbreeding avoidance, however, appears to be at least one mechanism maintaining genetic diversity in this population.
Endoplasmic reticulum stress and the unfolded protein response in skeletal muscle of subjects suffering from peritoneal sepsis
Authors: Metzing Uta Barbara , von Loeffelholz Christian , Steidl Ricardo , Romeike Bernd , Winkler René , Rauchfuß Falk , Settmacher Utz , Stoppe Christian , Coldewey Sina M. , Weinmann Claudia , Weickert Martin O. , Claus Ralf A. , Birkenfeld Andreas L. , Kosan Christian , Horn Paul
Published: January 2022

We provide a descriptive characterization of the unfolded protein response (UPR) in skeletal muscle of human patients with peritoneal sepsis and a sepsis model of C57BL/6J mice. Patients undergoing open surgery were included in a cross-sectional study and blood and skeletal muscle samples were taken. Key markers of the UPR and cluster of differentiation 68 (CD68) as surrogate of inflammatory injury were evaluated by real-time PCR and histochemical staining. CD68 mRNA increased with sepsis in skeletal muscle of patients and animals (p < 0.05). Mainly the inositol-requiring enzyme 1α branch of the UPR was upregulated as shown by elevated X-box binding-protein 1 (XBP1u) and its spliced isoform (XBP1s) mRNA (p < 0.05, respectively). Increased expression of Gadd34 indicated activation of PRKR-Like Endoplasmic Reticulum Kinase (PERK) branch of the UPR, and was only observed in mice (p < 0.001) but not human study subjects. Selected cell death signals were upregulated in human and murine muscle, demonstrated by increased bcl-2 associated X protein mRNA and TUNEL staining (p < 0.05). In conclusion we provide a first characterization of the UPR in skeletal muscle in human sepsis.
Insights into cell robustness against lignocellulosic inhibitors and insoluble solids in bioethanol production processes
Authors: Moreno Antonio D. , González-Fernández Cristina , Tomás-Pejó Elia
Published: January 2022

Increasing yeast robustness against lignocellulosic-derived inhibitors and insoluble solids in bioethanol production is essential for the transition to a bio-based economy. This work evaluates the effect exerted by insoluble solids on yeast tolerance to inhibitory compounds, which is crucial in high gravity processes. Adaptive laboratory evolution (ALE) was applied on a xylose-fermentingSaccharomyces cerevisiaestrain to simultaneously increase the tolerance to lignocellulosic inhibitors and insoluble solids. The evolved strain gave rise to a fivefold increase in bioethanol yield in fermentation experiments with high concentration of inhibitors and 10% (w/v) of water insoluble solids. This strain also produced 5% (P > 0.01) more ethanol than the parental in simultaneous saccharification and fermentation of steam-exploded wheat straw, mainly due to an increased xylose consumption. In response to the stress conditions (solids and inhibitors) imposed in ALE, cells induced the expression of genes related to cell wall integrity (SRL1,CWP2,WSC2andWSC4) and general stress response (e.g.,CDC5,DUN1,CTT1,GRE1), simultaneously repressing genes related to protein synthesis and iron transport and homeostasis (e.g.,FTR1,ARN1,FRE1), ultimately leading to the improved phenotype. These results contribute towards understanding molecular mechanisms that cells might use to convert lignocellulosic substrates effectively.
Do the total mercury concentrations detected in fish from Czech ponds represent a risk for consumers?
Authors: Pavla Sehonova , Danka Harustiakova , Premysl Mikula , Denisa Medkova , Kristyna Malacova , Zdenka Svobodova
Published: January 2022

Mercury is one of the important pollutants of the environment. Therefore, it’s necessary to monitor quantity of mercury especially in aquatic ecosystems. The main goal of the presented study was to compare the content of total mercury in tissues of fish coming from the Czech Republic, an important carp exporter, with focus on comparison of mercury content between 3 different ponds, its comparison between different fish species and between different tissues of the same species, and estimation whether the mercury content in tissues meets the limit given in the Commission Regulation (EC) No. 1881/2006 or not. Total mercury concentration was measured in 90 fish specimen sampled from three ponds (Velky Kocelovicky, Mysliv and Zehunsky) in autumn 2018. The values of total mercury in fish tissues was measured by atomic absorption spectrometry. The content of total mercury in the tissues decreased as follows: muscle > liver > gonads > scales. The highest average content of total mercury in muscle was 0.1517 ± 0.0176 mg/kg coming from pike caught in Velky Kocelovicky pond. In contrast, the lowest average content of total mercury in muscle 0.0036 ± 0.0003 mg/kg was found in carp tissue coming from the locality of Zehunsky pond. We confirmed that the predatory fish are more exposed to mercury than non-predatory fish. None of the monitored localities exceeded the set regulatory limit. Thus, our study shows that fish coming from these ponds are safe in terms of total mercury content.
Inter-personal motor interaction is facilitated by hand pairing
Authors: Mojtahedi Keivan , Kiani Kimia , Santello Marco , Fu Qiushi
Published: January 2022

The extent to which hand dominance may influence how each agent contributes to inter-personal coordination remains unknown. In the present study, right-handed human participants performed object balancing tasks either in dyadic conditions with each agent using one hand (left or right), or in bimanual conditions where each agent performed the task individually with both hands. We found that object load was shared between two hands more asymmetrically in dyadic than single-agent conditions. However, hand dominance did not influence how two hands shared the object load. In contrast, hand dominance was a major factor in modulating hand vertical movement speed. Furthermore, the magnitude of internal force produced by two hands against each other correlated with the synchrony between the two hands’ movement in dyads. This finding supports the important role of internal force in haptic communication. Importantly, both internal force and movement synchrony were affected by hand dominance of the paired participants. Overall, these results demonstrate, for the first time, that pairing of one dominant and one non-dominant hand may promote asymmetrical roles within a dyad during joint physical interactions. This appears to enable the agent using the dominant hand to actively maintain effective haptic communication and task performance.