bluuebunny/crossref_metadata_2025 huggingface dataset:
Two Nipped-B-Like Protein A (Nipbla) Gametologs in Chinese Tongue Sole (Cynoglossus semilaevis): The Identification of Alternative Splicing, Expression Pattern, and Promoter Activity Analysis
Authors: Zhu Ying , Shi Rui , Yang Qian , Zhang Mengqian , Chen Songlin , Wang Na
Published: March 2022

In mammals, the mutation of nipped-B-like protein (nipbl) leads to Cornelia de Lange Syndrome (CdLS), characterized by low birth weight, short stature, and structural abnormalities of the skeleton, heart, and gut. In Chinese tongue sole (Cynoglossus semilaevis), a typical marine fish exhibiting sexual size dimorphism, the nipbl homolog gene (nipped-B-like protein A (nipbla)) was also screened with female higher expression level by somatotropic and reproductive tissues’ transcriptomic analysis. In this study, two nipbla genes, namely, nipbla-w and nipbla-z, were identified from the W and Z chromosomes of C. semilaevis, respectively. Similar to other mammalian and fish species nipbl, the two homolog proteins of C. semilaevis contained two conserved domains, namely, cohesion_HEAT and Nipped-B_C. The phylogenetic tree analysis showed that these two nipbla gametolog proteins were first clustered together and then grouped with other fish species. At least two types of alternative splicing sites were observed in exon 12 of the nipbla-z gene, which produced nipbla-z-tv1 and nipbla-z-tv2. Also, the sex-biased expression patterns of different nipbla-w and nipbla-z transcripts in female and male tissues were revealed by quantitative PCR (qPCR). The highest expression level of nipbla-w was observed in female gonad. While nipbla-z-tv1 exhibited relatively high expression in the muscle, liver, gonad, and brain, nipbla-z-tv2 only showed its expression superiority in the muscle of male individuals. The promoter regions of nipbla genes were amplified, and their transcription activity was successfully verified by a dual-luciferase reporter system. After nipbla-w and nipbla-z knockdown in the brain cell lines by RNA interference, a series of growth-related genes were influenced, including Bone Morphogenetic Protein 4 (bmp4), Wnt Family Member 11 (wnt11), and Sprouty Related EVH1 Domain Containing 2 (spred2). The prediction of transcription factors suggested that c-Jun, sex-determining region Y (SRY), POU Class 1 Homeobox 1 (POU1F1a), myogenic differentiation antigen (MyoD), signal transducer and activator of transcription 5a (STAT5A), and nuclear factor I C (Nfic) might be the putative upstream regulatory factors for nipbla; among them, c-Jun has been verified to effectively regulate the transcriptional activity of nipbla. The identification of two nipbla genes provided important data for interpreting the sexual size dimorphism in C. semilaevis.
Long-Term Monitoring of Diel and Seasonal Rhythm of Dentex dentex at an Artificial Reef
Authors: Francescangeli Marco , Sbragaglia Valerio , del Rio Fernandez Joaquin , Trullols Enric , Antonijuan Josefina , Massana Immaculada , Prat Joana , Nogueras Cervera Marc , Mihai Toma Daniel , Aguzzi Jacopo
Published: March 2022

Behavioral rhythms are a key aspect of species fitness, since optimize ecological activities of animals in response to a constantly changing environment. Cabled observatories enable researchers to collect long-term biological and environmental data in real-time, providing relevant information on coastal fishes’ ecological niches and their temporal regulation (i.e., phenology). In this framework, the platform OBSEA (an EMSO Testing-Site in the NW coastal Mediterranean) was used to monitor the 24-h and seasonal occurrence of an ecologically iconic (i.e., top-predator) coastal fish species, the common dentex (Dentex dentex). By coupling image acquisition with oceanographic and meteorological data collection at a high-frequency (30 min), we compiled 8-years’ time-series of fish counts, showing daytime peaks by waveform analysis. Peaks of occurrence followed the photophase limits as an indication of photoperiodic regulation of behavior. At the same time, we evidenced a seasonal trend of counts variations under the form of significant major and minor increases in August and May, respectively. A progressive multiannual trend of counts increase was also evidenced in agreement with the NW Mediterranean expansion of the species. In GLM and GAM modeling, counts not only showed significant correlation with solar irradiance but also with water temperature and wind speed, providing hints on the species reaction to projected climate change scenarios. Grouping behavior was reported mostly at daytime. Results were discussed assuming a possible link between count patterns and behavioral activity, which may influence video observations at different temporal scales.
Research Advance in Manganese Nanoparticles in Cancer Diagnosis and Therapy
Authors: Nie Dengyun , Zhu Yinxing , Guo Ting , Yue Miao , Lin Mei
Published: March 2022

As the second reason of causing death after cardiovascular disease for human being, cancer is damaging people all over the world. Fortunately, rapidly developing in the past decade, nanotechnology has become one of the most promising technologies for cancer theranostics. Recent studies have demonstrated that metal nanoparticles, especially manganese nanoparticles (Mn-NPs), exhibit amazing potential for application in multifarious oncology fields according to their characteristic fundamental properties. Although global scientists have developed a variety of new Mn-NPs and have proved their preponderance in cancer diagnosis and treatment, Mn-NPs are still not approved for clinical use. In this paper, the recent research progress of Mn-NPs in the fields of cancer diagnosis and therapy is reviewed. Besides, the future prospect and challenges of Mn-NPs are discussed to explore wider applications of Mn-NPs in clinic. Here, we hope that this review will show a better overall understanding of Mn-NPs and provide guidance for their design in clinical applications for cancer.
Evolution of Biomaterials
Authors: Vallet-Regí Maria
Published: March 2022

There is a clear need for increasingly versatile and effective implantable biomaterials, and to train qualified personnel for research and development in the field of biomaterials design and manufacturing. In all these implantable biomaterials, science and technology are imposing new designs with combinations of new biomaterials, new coatings, and new design and manufacturing technologies (biomimetic biomaterials, functional biomaterials, nanotechnology, finite element modeling, additive manufacturing, 3D printing, tissue engineering, and drug delivery) that will revolutionize the field of implants in the short term. Biomaterials are part of biomedical engineering and bring together knowledge from the world of science, engineering, biology, and medicine, being a multidisciplinary field where borders have no place.
Retinal and Choroidal Vascular Perfusion and Thickness Measurement in Diabetic Retinopathy Patients by the Swept-Source Optical Coherence Tomography Angiography
Authors: Liu Tingting , Lin Wei , Shi Genggeng , Wang Wenqi , Feng Meng , Xie Xiao , Liu Tong , Zhou Qingjun
Published: March 2022

Purpose

To observe the changes in retinal and choroidal microstructures in patients with different stages of diabetic retinopathy (DR) and to evaluate the vascular perfusion of retina and choroid retinal thickness, retinal and choroidal vessel density by the swept-source optical coherence tomography angiography (SS-OCTA).

Methods

Subjects were divided into three groups: healthy control group (30 cases, 51 eyes), non-proliferative diabetic retinopathy (NPDR, 42 cases, 71 eyes) and proliferative diabetic retinopathy (PDR, 31 cases, 53 eyes). The area of the foveal avascular zone (FAZ), retinal and choroidal vascular perfusion, and the deep vascular complexes, including the intermediate capillary plexus (ICP) and deep capillary plexus (DCP) within the radius of 3, 6, 9, and 12 mm around the fovea were measured by SS-OCTA.

Results

Compared with the healthy control group, DR patients presented significantly increased fovea avascular area, while vessel density (VD) in the ICP and DCP, vascular perfusion rate, and the retinal thickness were considerably decreased. There were significant differences in the retinal thickness, ICP, and DCP vessel densities between the control and NPDR groups, or control and PDR groups, or PDR and NPDR groups. The deep vascular perfusion rate also significantly differed between the control and PDR groups, but there was no significant difference between the PDR and NPDR groups. The choroidal perfusion exhibited significant differences across different areas and amongst the three groups. Furthermore, the choroidal thickness showed a significant difference between the PDR and NPDR groups.

Conclusion

Our results showed significant differences in the area of the avascular fovea and the VD of deep vascular complexes between DR patients and healthy control subjects. Moreover, there were significant differences in retinal VD, especially in the deep-retinalregions, choroidal perfusion, and the volume of large vessel choroid in DR patients with different degrees of disease severity. Notably, SS-OCTA provided in-depth information for detecting the potential VD damage in DR patients caused by a multitudeof factors.
Development and Validation of a Prognostic Nomogram in Lung Cancer With Obstructive Sleep Apnea Syndrome
Authors: Liu Wei , Zhou Ling , Zhao Dong , Wu Xiaofeng , Yue Fang , Yang Haizhen , Jin Meng , Xiong Mengqing , Hu Ke
Published: March 2022

To analyze the prognostic factors and survival rate of lung cancer patients with obstructive sleep apnea (OSA) by nomogram. The nomogram was established by a development cohort (n = 90), and the validation cohort included 38 patients. Factors in the nomogram were identified by Cox hazard analysis. We tested the accuracy of the nomograms by discrimination and calibration, and plotted decision curves to assess the benefits of nomogram-assisted decisions. There were significant difference in sex, apnea hypopnea index (AHI), Tumor Node Metastasis (TNM), coronary heart disease, lowest arterial oxygen saturation [LSpO2 (%)], oxygen below 90% of the time [T90% (min)], the percentage of the total recorded time spend below 90% oxygen saturation (TS90%) and oxygen desaturation index (ODI4) between lung cancer subgroup and lung cancer with OSA subgroup (P < 0.05). Lung cancer patients with OSA age, AHI, TNM, cancer types, BMI and ODI4 were independent prognostic factor. Based on these six factors, a nomogram model was established. The c-index of internal verification was 0.802 (95% CI 0.767–0.885). The ROC curve analysis for the nomogram show 1-year survival (AUC = 0.827), 3-year survival (AUC = 0.867), 5-year survival (AUC = 0.801) in the development cohort were good accuracy. The calibration curve shows that this prediction model is in good agreement. Decision curve analysis (DCA) suggests that the net benefit of decision-making with this nomogram is higher, especially in the probability interval of <20% threshold. The nomogram can predict the prognosis of patients and guide individualized treatment.
Description of an Integrated and Dynamic System to Efficiently Deal With a Raging COVID-19 Pandemic Peak
Authors: Agnoletti Vanni , Gamberini Emiliano , Circelli Alessandro , Martino Costanza , Santonastaso Domenico Pietro , Bolondi Giuliano , Bastoni Giorgia , Spiga Martina , Ceccarelli Paola , Montaguti Luca , Catena Fausto , Poletti Venerino , Lusenti Carlo , Lazzari Claudio , Altini Mattia , Russo Emanuele
Published: March 2022

Background

This study aimed to describe an innovative and functional method to deal with the increased COVID-19 pandemic-related intensive care unit bed requirements.

Methods

We described the emergency creation of an integrated system of internistic ward, step-down unit, and intensive care unit, physically located in reciprocal vicinity on the same floor. The run was carried out under the control of single intensive care staff, through sharing clinical protocols and informatics systems, and following single director supervision. The intention was to create a dynamic and flexible system, allowing for rapid and fluid patient admission/discharge, depending on the requirements due to the third Italian peak of the COVID-19 pandemic in March 2021.

Results

This study involved 142 COVID-19 patients and 66 non-COVID-19 patients who were admitted; no critical patient was left unadmitted and no COVID-19 severe patients referring to our center had to be redirected to other hospitals due to bed saturation. This system allowed shorter hospital length-of-stay in general wards (5.9 ± 4 days) than in other internistic COVID-19 wards and overall mortality in line with those reported in literature despite the peak raging.

Conclusion

This case report showed the feasibility and the efficiency of this dynamic model of hospital rearrangement to deal with COVID-19 pandemic peaks.
Gene Variation at Immunomodulatory and Cell Adhesion Molecules Loci Impacts Primary Sjögren's Syndrome
Authors: Casadó-Llombart Sergi , Gheitasi Hoda , Ariño Silvia , Consuegra-Fernández Marta , Armiger-Borràs Noelia , Kostov Belchin , Ramos-Casals Manuel , Brito-Zerón Pilar , Lozano Francisco
Published: March 2022

Primary Sjögren's syndrome (pSS) is an autoimmune disease triggered by a combination of environmental and host genetic factors, which results in the focal lymphocytic infiltration of exocrine glands causing eye and mouth dryness. Glandular infiltrates include T and B cell subsets positive for CD5 and/or CD6, two surface scavenger receptors involved in the fine-tuning of intracellular signals mediated by the antigen-specific receptor complex of T (TCR) and B (BCR) cells. Moreover, the epithelial cells of inflamed glands overexpress CD166/ALCAM, a CD6 ligand involved in homo and heterotypic cell adhesion interactions. All this, together with the reported association of functionally relevant single nucleotide polymorphisms (SNPs) ofCD5, CD6, andCD166/ALCAMwith the risk or prognosis of some immune-mediated inflammatory disorders, led us to investigate similar associations in a local cohort of patients with pSS. The logistic regression analyses of individual SNPs showed the association ofCD5rs2241002Twith anti-Ro/La positivity,CD6rs17824933Cwith neutropenia, andCD6rs11230563Twith increased leukopenia and neutropenia but decreased peripheral nervous system EULAR Sjögren's syndrome disease activity index (ESSDAI). Further analyses showed the association of haplotypes fromCD5(rs2241002T-rs2229177C) with anemia and thrombocytopenia,CD6(rs17824933G-rs11230563C-rs12360861G) with cutaneous ESSDAI, andCD166/ALCAM(rs6437585C-rs579565A-rs1044243Cand rs6437585C-rs579565G-rs1044243T) with disease susceptibility and several analytical parameters (anti-nuclear antibodies, neurological ESSDAI, and hematologic cytopenias). These results support the relevance of gene variation at loci coding for cell surface receptors involved in the modulation of T and B lymphocyte activation (CD5, CD6) and epithelial-immune cell adhesion (CD166/ALCAM) in modulating the clinical and analytical outcomes in patients with pSS.
Early Menopause May Associate With a Higher Risk of CKD and All-Cause Mortality in Postmenopausal Women: An Analysis of NHANES, 1999–2014
Authors: Qian Duo , Wang Zu-feng , Cheng Yi-chun , Luo Ran , Ge Shu-Wang , Xu Gang
Published: March 2022

Background

Chronic kidney disease (CKD) in women is often accompanied by hormone disorders such as sex hormones, and most women with CKD are in the post-menopausal age group. Due to the close relationship between menopause and sex hormones, we aimed to explore the association between early menopause and CKD in post-menopausal women, and the influence of early menopause on longevity in the CKD population.

Methods

Information regarding 4,945 post-menopausal women was extracted from the database of the National Health and Nutrition Examination Survey (NHANES) 1999–2014, and then divided into 4 groups according to the type of menopause (natural or surgical) and early menopause (menopause at age <45) or not. The association between early menopause and CKD prevalence was examined using multivariable logistic regression, while we used multivariable Cox proportional hazards models to investigate the possible relationship between early menopause and all-cause mortality in CKD and non-CKD populations. The differences in the levels of sex hormones between women with and without CKD were also explored.

Results

Compared with women with natural menopause
at age ≥45, women experiencing early natural menopause had a higher risk of CKD [OR = 1.26 (1.01–1.56)]. Similarly, as compared to women with surgical menopause at age ≥ 45, women in the early surgical menopause group were more likely to have CKD [OR = 1.38 (1.05–1.81)]. In addition, early surgical menopause was associated with higher mortality in the non-CKD group [HR = 1.62 (1.06–2.49)], but not in the CKD group. Women with CKD had a higher level of luteinizing hormone and follicle-stimulating hormone, combined with a lower level of testosterone and estradiol than the non-CKD women.

Conclusion

Both early natural and surgical menopause were associated with a higher risk of CKD. Early surgical menopause was a hazard factor for survival in the non-CKD group, but not in the CKD group. Further research is required to understand the mechanisms.
Peripapillary Vascular Reactivity in Primary Open-Angle Glaucoma With High Myopia by Using Optical Coherence Tomography Angiography
Authors: Fan Xintong , Xu Huan , Zhai Ruyi , Sheng Qilian , Sun Yanan , Shao Tingting , Kong Xiangmei
Published: March 2022

Purpose

To evaluate peripapillary vascular reactivity in primary open-angle glaucoma (POAG) with and without high myopia (HM) by using optical coherence tomography angiography (OCTA).

Methods

This prospective study enrolled 48 eyes with POAG, including 16 and 32 eyes with and without HM, respectively. The retinal peripapillary vessel density (VD) was repeatedly assessed using OCTA at baseline and after a hyperoxia test (breathing 80% oxygen). The VD changes between different oxygenation conditions were calculated to reflect the vasoreactivity. Linear regression was performed to determine the relationship between myopia and retinal vascular reactivity in patients with POAG. Systemic hemodynamic characteristics were also evaluated under both conditions.

Results

The VD was significantly reduced after hyperoxia in the whole image (baseline and hyperoxia: 41.4 ± 4.5 and 38.8 ± 4.4, respectively, P < 0.001) and in the peripapillary regions (44.3 ± 5.7 and 41.1 ± 5.4, respectively, P < 0.001) in POAG eyes without HM. However, in eyes with HM, the whole-image VD in hyperoxia was not significantly different from the baseline (baseline and hyperoxia: 40.5 ± 6.2 and 40.2 ± 6.2, respectively, P = 0.481). The VD changes in eyes with HM were significantly smaller than those in eyes without HM in both the whole image (0.3 ± 1.8 and 2.6 ± 2.0, respectively, P < 0.001) and peripapillary regions (1.1 ± 2.0 and 3.2 ± 2.3, respectively, P = 0.003). Linear regression results showed a significant correlation between retinal vascular reactivity and spherical equivalent (SE) (β = 0.28, P < 0.001, R2 = 0.31) and axial length (AL) (β = −0.72, P < 0.001, R2 = 0.33).

Conclusion

Retinal vasoreactivity of peripapillary capillaries in POAG eyes with HM was significantly impaired in comparison with that in POAG eyes without HM. A lower peripapillary vascular response was significantly associated with worse SE and elongated AL.
Bioleaching of Chalcopyrite Waste Rock in the Presence of the Copper Solvent Extractant LIX984N
Authors: Liu Xiaohui , Li Yuhong , Cao Jianfeng , Zeng Zhenshun , Liu Xiaorong , Zhang Ruiyong , Li Qian , Sand Wolfgang
Published: March 2022

Heap bioleaching, the solubilization of metal ions from metal sulfides by microbial oxidation, is often combined with solvent extraction (SX) and electrowinning to recover, e.g., copper from low-grade ores. After extraction, the leaching solution is recycled, but the entrained organic solvents may be toxic to the microorganisms. Here Acidithiobacillus ferrooxidans, Leptospirillum ferrooxidans, and Sulfobacillus thermosulfidooxidans were selected to perform bioleaching of chalcopyrite waste rock in the presence of the SX reagent (2.5% v/v LIX984N in kerosene). Possibly inhibitory effects have been evaluated by copper extraction, bacterial activity, number of actively Fe(II)-oxidizing cells, and biofilm formation. Microcalorimetry, most probable number determination, and atomic force microscopy combined with epifluorescence microscopy were applied. The results show that 100 and 300 mg/L SX reagent could hardly inhibit At. ferrooxidans from oxidizing Fe2+, but they seriously interfered with the biofilm formation and the oxidization of sulfur, thereby hindering bioleaching. L. ferrooxidans was sensitive to 50 mg/L SX reagent, which inhibited its bioleaching completely. Sb. thermosulfidooxidans showed different metabolic preferences, if the concentration of the SX reagent differed. With 10 mg/L LIX984N Sb. thermosulfidooxidans preferred to oxidize Fe2+ and extracted the same amount of copper as the assay without LIX984N. With 50 mg/L extractant the bioleaching stopped, since Sb. thermosulfidooxidans preferred to oxidize reduced inorganic sulfur compounds.
Migration Drives the Replacement of Xanthomonas perforans Races in the Absence of Widely Deployed Resistance
Authors: Bernal Eduardo , Rotondo Francesca , Roman-Reyna Veronica , Klass Taylor , Timilsina Sujan , Minsavage Gerald V. , Iruegas-Bocardo Fernanda , Goss Erica M. , Jones Jeffrey B. , Jacobs Jonathan M. , Miller Sally A. , Francis David M.
Published: March 2022

Changes in Xanthomonas race and species composition causing bacterial spot of tomato have occurred throughout the world and are often associated with epidemics. Knowledge of bacterial population structure is key for resistance discovery and deployment. We surveyed Xanthomonas spp. composition from processing tomato fields in the Midwestern United States over a 4-year period between 2017 and 2020, compared these to strains collected previously, and found that X. perforans is currently the most prevalent species. We characterized 564 X. perforans isolates for sequence variation in avrXv3 to distinguish between race T3 and T4 and validated race designation using hypersensitive response (HR) assays for 106 isolates. Race T4 accounted for over 95% of X. perforans isolates collected in the Midwest between 2017 and 2020. Whole genome sequencing, Average Nucleotide Identity (ANI) analysis, core genome alignment and single nucleotide polymorphism (SNP) detection relative to a reference strain, and phylogenomic analysis suggest that the majority of Midwestern X. perforans strains collected between 2017 and 2020 were nearly identical, with greater than 99.99% ANI to X. perforans isolates collected from Collier County, Florida in 2012. These isolates shared a common SNP variant resulting an a premature stop codon in avrXv3. One sequenced isolate was identified with a deletion of avrXv3 and shared 99.99% ANI with a strain collected in Collier Co., Florida in 2006. A population shift to X. perforans T4 occurred in the absence of widely deployed resistance, with only 7% of tomato varieties tested having the resistant allele at the Xv3/Rx-4 locus. The persistence of nearly identical strains over multiple years suggests that migration led to the establishment of an endemic population. Our findings validate a genomics-based framework to track shifts in X. perforans populations due to migration, mutation, drift, or selection based on comparisons to 146 genomes.
Composition and Functional Diversity of Epiphytic Bacterial and Fungal Communities on Marine Macrophytes in an Intertidal Zone
Authors: Chen Jun , Zang Yu , Yang Zhibo , Qu Tongfei , Sun Tao , Liang Shuo , Zhu Meiling , Wang Ying , Tang Xuexi
Published: March 2022

Marine macrophytes (seagrasses and macroalgae) and their epiphytic microorganisms play an important role in the ecological and biochemical processes of coastal oceans. However, simultaneous comparative studies on the biodiversity and functions of epiphytic bacteria and fungi associated with marine macrophytes have not been conducted. In this study, high-throughput sequencing technology was used to describe the epiphytic bacterial and fungal communities of 11 common macroalgae and 2 seagrasses from an intertidal zone of northern China and compare them with seawater communities. The results showed that Proteobacteria and Bacteroidota were the dominant bacterial phyla in marine macrophytes, whereas Ascomycota, Chytridiomycota, and Basidiomycota were the dominant fungal phyla. The alpha diversity of the bacterial and fungal communities in seagrasses was the highest of all macrophyte samples. This may have been related to their ability to recruit microorganisms from multiple sources. Host phylogeny may influence bacterial community structure, and geographical differences may influence fungal community structure. The FAPROTAX data indicated that C metabolic microbes were enriched in marine macrophytes, while the FUNGuild data indicated that undefined saprotroph, which participated in organic matter degradation, were also enriched in marine macrophytes. These findings provide a theoretical basis regarding the epiphytic microorganisms of macrophytes and may offer new insights to support the improved ecological restoration of seagrass and macroalgae beds.
Metabolic Mechanism of Sulfadimethoxine Biodegradation by Chlorella sp. L38 and Phaeodactylum tricornutum MASCC-0025
Authors: Li Bing , Wu Di , Li Yan , Shi Yan , Wang Chenlin , Sun Jiasi , Song Chunfeng
Published: March 2022

Antibiotic resistance is one of the most important environmental challenges. Microalgae has been considered as a promising green media for environmental purification. In this work, sulfadimethoxine (SDM) biodegradation potential of Chlorella sp. L38 and Phaeodactylum tricornutum MASCC-0025 is investigated. Experimental results indicated that the tested freshwater and marine microalgae strains presented stress response to SDM addition. For Chlorella sp. L38, it has a good adaptability to SDM condition via antioxidant enzyme secretion (SOD, MDA, and CAT up to 23.27 U/mg, 21.99 μmol/g, and 0.31 nmol/min/mg) with removal rate around 88%. P. tricornutum MASCC-0025 exhibited 100% removal of 0.5 mg/L SDM. With increasing salinity (adding a certain amount of NaCl) of cultivation media, the removal rate of SDM by microalgae increased. Although its adaptive process was slower than Chlorella sp. L38, the salinity advantage would facilitate enzyme accumulation. It indicated that microalgae could be used to remove SDM from freshwater and marine environment via suitable microalgae strain screening.
Differences in Bioenergetic Metabolism of Obligately Alkaliphilic Bacillaceae Under High pH Depend on the Aeration Conditions
Authors: Goto Toshitaka , Ogami Shinichi , Yoshimume Kazuaki , Yumoto Isao
Published: March 2022

AlkaliphilicBacillaceaeappear to produce ATP based on the H+-based chemiosmotic theory. However, the bulk-based chemiosmotic theory cannot explain the ATP production in alkaliphilic bacteria because the H+concentration required for driving ATP synthesis through the ATPase does not occur under the alkaline conditions. Alkaliphilic bacteria produce ATP in an H+-diluted environment by retaining scarce H+extruded by the respiratory chain on the outer surface of the membrane and increasing the potential of the H+for ATP production on the outer surface of the membrane using specific mechanisms of ATP production. Under high-aeration conditions, the high ΔΨ (ca. -170 mV) of the obligate alkaliphilicEvansella clarkiiretains H+at the outer surface of the membrane and increases the intensity of the protonmotive force (Δp) per H+across the membrane. One of the reasons for the production of high ΔΨ is the Donnan potential, which arises owing to the induction of impermeable negative charges in the cytoplasm. The intensity of the potential is further enhanced in the alkaliphiles compared with neutralophiles because of the higher intracellular pH (ca. pH 8.1). However, the high ΔΨ observed under high-aeration conditions decreased (∼ -140 mV) under low-aeration conditions.E.clarkiiproduced 2.5–6.3-fold higher membrane bound cytochromecin the content of the cell extract under low-aeration conditions than under high-aeration conditions. The predominant membrane-bound cytochromecin the outer surface of the membrane possesses an extra Asn-rich segment between the membrane anchor and the main body of protein. This structure may influence the formation of an H+-bond network that accumulates H+on the outer surface of the membrane. Following accumulation of the H+-bond network producing cytochromec,E.clarkiiconstructs an H+capacitor to overcome the energy limitation of low aeration at high pH conditions.E.clarkiiproduces more ATP than other neutralophilic bacteria by enhancing the efficacy per H+in ATP synthesis. In low H+environments,E.clarkiiutilizes H+efficiently by taking advantage of its high ΔΨ under high-aeration conditions, whereas under low-aeration conditionsE.clarkiiuses cytochromecbound on its outer surface of the membrane as an H+capacitor.
Influence of Transcranial Direct Current Stimulation Dosage and Associated Therapy on Motor Recovery Post-stroke: A Systematic Review and Meta-Analysis
Authors: Chow Alan-Michael D. , Shin Jeonghwa , Wang Hongwu , Kellawan Jeremy Mikhail , Pereira Hugo M.
Published: March 2022

Purpose

(1) To determine the impact of transcranial direct current stimulation (tDCS) applied alone or combined with other therapies on the recovery of motor function after stroke and (2) To determine tDCS dosage effect.

Methods

Randomized controlled trials comparing the effects of tDCS with sham, using the Barthel Index (BI), the upper and lower extremity Fugl–Meyer Assessment (FMA), and the Modified Ashworth Scale (MAS), were retrieved from PubMed, Medline (EBSCO), and Cumulative Index to Nursing and Allied Health Literature (CINAHL) from their inception to June 2021. Calculations for each assessment were done for the overall effect and associated therapy accounting for the influence of stroke severity or stimulation parameters.

Results

A total of 31 studies involving metrics of the BI, the upper extremity FMA, the lower extremity FMA, and the MAS were included. tDCS combined with other therapies was beneficial when assessed by the BI (mean difference: 6.8; P < 0.01) and these studies typically had participants in the acute stage. tDCS effects on the upper and lower extremity FMA are unclear and differences between the sham and tDCS groups as well as differences in the associated therapy type combined with tDCS potentially influenced the FMA results. tDCS was not effective compared to sham for the MAS. Stimulation types (e.g., anodal vs. cathodal) did not influence these results and dosage parameters were not associated with the obtained effect sizes. Conventional therapy associated with tDCS typically produced greater effect size than assisted therapy. The influence of stroke severity is unclear.

Conclusion

Potential benefits of tDCS can vary depending on assessment tool used, duration of stroke, and associated therapy. Mechanistic studies are needed to understand the potential role of stimulation type and dosage effect after stroke. Future studies should carefully conduct group randomization, control for duration of stroke, and report different motor recovery assessments types.

Systematic Review Registration

[https://www.crd.york.ac.uk/PROSPERO/], identifier [CRD42021290670].
LEACH Protocol Optimization Based on Weighting Strategy and the Improved Ant Colony Algorithm
Authors: Cheng Xuezhen , Xu Chuannuo , Liu Xiaoqing , Li Jiming , Zhang Junming
Published: March 2022

This article aims to address problems in the current clustering process of low-energy adaptive clustering hierarchy (LEACH) in the wireless sensor networks, such as strong randomness and local optimum in the path optimization. This article proposes an optimal combined weighting (OCW) and improved ant colony optimization (IACO) algorithm for the LEACH protocol optimization. First, cluster head nodes are updated via a dynamic replacement mechanism of the whole network cluster head nodes to reduce the network energy consumption. In order to improve the quality of the selected cluster head nodes, this article proposes the OCW method to dynamically change the weight according to the importance of the cluster head node in different regions, in accordance with the three impact factors of the node residual energy, density, and distance between the node and the sink node in different regions. Second, the network is partitioned and the transmission path among the clusters can be optimized by the transfer probability in IACO with combined local and global pheromone update mechanism. The efficacy of the proposed LEACH protocol optimization method has been verified with MATLAB simulation experiments.
A Personalized Spring Network Representation of Emphysematous Lungs From CT Images
Authors: Yuan Ziwen , Herrmann Jacob , Murthy Samhita , Peters Kevin , Gerard Sarah E. , Nia Hadi T. , Lutchen Kenneth R. , Suki Béla
Published: March 2022

Emphysema is a progressive disease characterized by irreversible tissue destruction and airspace enlargement, which manifest as low attenuation area (LAA) on CT images. Previous studies have shown that inflammation, protease imbalance, extracellular matrix remodeling and mechanical forces collectively influence the progression of emphysema. Elastic spring network models incorporating force-based mechanical failure have been applied to investigate the pathogenesis and progression of emphysema. However, these models were general without considering the patient-specific information on lung structure available in CT images. The aim of this work was to develop a novel approach that provides an optimal spring network representation of emphysematous lungs based on the apparent density in CT images, allowing the construction of personalized networks. The proposed method takes into account the size and curvature of LAA clusters on the CT images that correspond to a pre-stressed condition of the lung as opposed to a naïve method that excludes the effects of pre-stress. The main findings of this study are that networks constructed by the new method 1) better preserve LAA cluster sizes and their distribution than the naïve method; and 2) predict different course of emphysema progression compared to the naïve method. We conclude that our new method has the potential to predict patient-specific emphysema progression which needs verification using clinical data.
Impaired Cerebral Autoregulation in Parkinson's Disease: An Orthostatic Hypotension Analysis
Authors: Xing Yingqi , Li Qing , Xu Erhe , Zeng Jingrong , Li Qiuping , Mei Shanshan , Hua Yang
Published: March 2022

Orthostatic hypotension (OH) is an early non-motor manifestation of Parkinson's disease (PD). However, the underlying mechanism of hemodynamic changes in patients with PD and OH remains unclear. This study aimed to investigate the dynamic cerebral autoregulation changes in patients with PD with OH. Ninety patients with PD and 20 age- and sex-matched healthy controls (HCs) were recruited. The patients' non-invasive blood pressure (BP) and cerebral blood flow velocity were simultaneously recorded at supine and orthostatic positions during the active standing test (AST). Transfer function analysis was used to determine autoregulatory parameters including gain [i.e., damping effect of dynamic cerebral autoregulation (dCA) on the magnitude of BP oscillation] and phase difference (i.e., the time delay of the cerebral blood flow response to BP). Sixteen patients (17.8%) in the PD population were diagnosed with OH (PD-OH). The AST results were normal for 74 patients (82.2%) (PD-NOR). In the supine position, the PD-OH group had a lower phase degree than the PD-NOR group (50.3 ± 23.4 vs. 72.6 ± 32.2 vs. 68.9 ± 12.1, p = 0.020); however, no significant difference was found upon comparing with the HC group. In the orthostatic position, the normalized gain was significantly higher for the symptomatic OH group than for the asymptomatic OH group and HC group (1.50 ± 0.58 vs. 0.97 ± 0.29 vs. 1.10 ± 0.31, p = 0.019). A symptomatic OH in the PD population indicates an impaired cerebral autoregulation ability in the orthostatic position. Cerebral autoregulation tends to be impaired in the supine position in the OH population.
Evaluation of the Psychometric Properties of Jebsen Taylor Hand Function Test (JTHFT) in Italian Individuals With Multiple Sclerosis
Authors: Berardi Anna , Galeoto Giovanni , Pasquali Federica , Baione Viola , Crisafulli Sebastiano Giuseppe , Tofani Marco , Tartaglia Matteo , Fabbrini Giovanni , Conte Antonella
Published: March 2022

Introduction

The Jebsen Taylor Hand Function Test (JTHFT) is a non-diagnostic assessment scale for hand and upper limb dexterity that is commonly used in various countries around the world for diseases, such as muscular dystrophy, stroke, spinal cord injury, Parkinson, carpal tunnel syndrome, and rheumatoid arthritis. This study aimed to evaluate the psychometric properties of the JTHFT in Italian adults with Multiple Sclerosis (MS).

Materials and Methods

The test's internal consistency was evaluated with Cronbach's alpha, whereas its concurrent validity was evaluated by comparing the JTHFT with the Health Assessment Questionnaire (HAQ) and by calculating Pearson's correlation coefficient.

Results

The JTHFT was administered to 29 Italians with MS. The Cronbach's alpha showed that the nondominant hand has a value of 0.76 and 0.91 for the dominant hand. Pearson's correlation coefficient showed significant correlations between JTHFT and HAQ.

Discussion

The JTHFT is a reliable tool to evaluate the functionality of the upper limb and hand in patients with MS. This tool is useful for testing the effectiveness of a treatment in various diseases. The results obtained in this study are coherent with previous studies that are conducted in populations with different diseases. In particular, the correlation between JTHFT and HAQ showed that a disability related to the upper limbs can often have repercussions, not only on activities of daily living, but also on walking. Based on this correlation, the motor deficits that emerged may be linked to a brain marrow disease rather than a spinal disease, even if an essential deepening can confirm this hypothesis.
Automated Classification of Resting-State fMRI ICA Components Using a Deep Siamese Network
Authors: Chou Yiyu , Chang Catie , Remedios Samuel W. , Butman John A. , Chan Leighton , Pham Dzung L.
Published: March 2022

Manual classification of functional resting state networks (RSNs) derived from Independent Component Analysis (ICA) decomposition can be labor intensive and requires expertise, particularly in large multi-subject analyses. Hence, a fully automatic algorithm that can reliably classify these RSNs is desirable. In this paper, we present a deep learning approach based on a Siamese Network to learn a discriminative feature representation for single-subject ICA component classification. Advantages of this supervised framework are that it requires relatively few training data examples and it does not require the number of ICA components to be specified. In addition, our approach permits one-shot learning, which allows generalization to new classes not seen in the training set with only one example of each new class. The proposed method is shown to out-perform traditional convolutional neural network (CNN) and template matching methods in identifying eleven subject-specific RSNs, achieving 100% accuracy on a holdout data set and over 99% accuracy on an outside data set. We also demonstrate that the method is robust to scan-rescan variation. Finally, we show that the functional connectivity of default mode and salience networks identified by the proposed technique is altered in a group analysis of mild traumatic brain injury (TBI), severe TBI, and healthy subjects.
Ear Asymmetry and Contextual Influences on Speech Perception in Hearing-Impaired Patients
Authors: Moulin Annie
Published: March 2022

The left hemisphere preference for verbal stimuli is well known, with a right ear (RE) advantage obtained when competing verbal stimuli are presented simultaneously, at comfortable intensities, to both ears. Speech perception involves not only the processing of acoustic peripheral information but also top–down contextual influences, filling the gaps in the incoming information that is particularly degraded in hearing-impaired individuals. This study aimed to analyze the potential asymmetry of those contextual influences on a simple speech perception task in hearing-impaired patients in light of hemispheric asymmetry. Contextual influences on disyllabic word perception scores of 60 hearing-impaired patients were compared between left ear (LE) and RE, in a balanced design, involving two repetitions of the same task. Results showed a significantly greater contextual influence on the REversusthe LE and, for the second repetitionversusthe first one, without any interaction between the two. Furthermore, the difference in contextual influences between RE and LE increased significantly with the RE advantage measured by a dichotic listening test in the absence of any significant correlation with hearing threshold asymmetry. Lastly, the contextual influence asymmetry decreased significantly as age increased, which was mainly due to a greater increase, with age, of contextual influences on the LEversusthe RE. Those results agree with the literature reporting a relative right-shift of hemispheric asymmetry observed with age in speech in noise perception tasks in normal hearing subjects and the clinical reports of generally better audiometric speech scores obtained in REversusLE.
Dynamic Transitions Between Brain States Predict Auditory Attentional Fluctuations
Authors: Kondo Hirohito M. , Terashima Hiroki , Ezaki Takahiro , Kochiyama Takanori , Kihara Ken , Kawahara Jun I.
Published: March 2022

Achievement of task performance is required to maintain a constant level of attention. Attentional level fluctuates over the course of daily activities. However, brain dynamics leading to attentional fluctuation are still unknown. We investigated the underlying mechanisms of sustained attention using functional magnetic resonance imaging (fMRI). Participants were scanned with fMRI while performing an auditory, gradual-onset, continuous performance task (gradCPT). In this task, narrations gradually changed from one to the next. Participants pressed a button for frequent Go trials (i.e., male voices) as quickly as possible and withheld responses to infrequent No-go trials (i.e., female voices). Event-related analysis revealed that frontal and temporal areas, including the auditory cortex, were activated during successful and unsuccessful inhibition of predominant responses. Reaction-time (RT) variability throughout the auditory gradCPT was positively correlated with signal changes in regions of the dorsal attention network: superior frontal gyrus and superior parietal lobule. Energy landscape analysis showed that task-related activations could be clustered into different attractors: regions of the dorsal attention network and default mode network. The number of alternations between RT-stable and erratic periods increased with an increase in transitions between attractors in the brain. Therefore, we conclude that dynamic transitions between brain states are closely linked to auditory attentional fluctuations.
Association Between Ideal Cardiovascular Health and Vegetarian Dietary Patterns Among Community-Dwelling Individuals
Authors: He Yu-Min , Chen Wei-Liang , Kao Tung-Wei , Wu Li-Wei , Yang Hui-Fang , Peng Tao-Chun
Published: March 2022

Background

Vegetarians have been shown to have better metabolic profiles than non-vegetarians, and vegetarianism has potential beneficial effects on cardiovascular disease. However, there is a lack of studies on vegetarians that examine both metabolic profiles and lifestyle habits, such as physical activity, smoking habits, and dietary patterns, which are equally important in the context of cardiovascular disease. We explored whether a vegetarian diet is associated with both metabolic traits and lifestyle habits by assessing cardiovascular health (CVH) metrics.

Methods

This was a cross-sectional study conducted in a Taiwanese population. Data collected between 2000 and 2016 were extracted from the MJ Health database. Participants aged 40 years and older without cardiovascular disease were included. CVH metrics included smoking habits, blood pressure, total cholesterol, serum glucose, body mass index, physical activity, and healthy diet score. Vegetarian participants were full-time vegetarians who did not consume meat or fish. All the data were assessed from self-report questionnaires, physical examinations, and blood analyses following standard protocol. Multiple logistic regression analysis was used to evaluate the association between vegetarianism and CVH metrics.

Results

Of 46,287 eligible participants, 1,896 (4.1%) were vegetarian. Overall, vegetarians had better CVH metrics (OR = 2.09, 95% CI = 1.84–2.37) but lower healthy diet scores (OR = 0.41, 95% CI = 0.33–0.51) after adjustment. No difference in physical activity (OR = 0.86, 95% CI = 0.73–1.02) was identified between vegetarians and non-vegetarians. Additionally, vegetarians had higher whole grain intake (OR = 2.76, 95% CI = 2.28–3.35) and lower sugar-sweetened beverage consumption (OR = 1.36, 95% CI = 1.18–1.58).

Conclusions

Our results suggested that vegetarians had better overall ideal CVH metrics but lower ideal healthy diet scores than non-vegetarians, which was likely due to the lack of fish consumption in this population group. When assessing CVH metrics and healthy diet scores for vegetarians, metrics and scores chosen should be suitable for use with vegetarian populations.