bluuebunny/crossref_metadata_2025 huggingface dataset:
Penerapan Asas Sederhana, Cepat, Biaya Ringan Dalam Penyelesaian Sengketa Pegadaian Syariah Melalui Basyarnas (Badan Arbitrase Syariah Nasional)
Authors: Alaqoh Shofwatul , Mujib Abdul
Published: November 2023

ABSTRAKPenelitian ini bertujuan untuk mengetahui bagaimana mekanisme penyelesiana sengketa pegadaian syariah melalui (Badan Arbitrase Syariah Nasional) BASYARNAS, apakah telah menerapkan asas sederhana, cepat dan biaya ringan. Melihat proses yang sederhana, dengan kurun waktu yang cepat dan biaya yang ringan merupakan hak untuk para pihak yang sedang bersengketa, dalam melakukan proses penyelesaian sengketa pegadaian syariah, Metode yang digunakan dalam penelitian ini adalah metode kualitatif yang merupakan metode pengumpulan data dengan menggunakan metode berfikir deduktif yang berangkat dari pengetahuan yang bersifat umum untuk menemukan kesimpulan yang bersifat khusus, serta mengumpulkan data dari sebuah deskripsi bukan angka, dengan menggunakan data-data, jurnal-jurnal ilmiah, dan sumber-sumber lain yang berkaitan dengan permasalahan yang diteliti. Hasil dari penelitian ini adalah, asas sederhana, cepat, dan biaya ringan telah diterapkan dalam penyelesaian sengketa pegadaian syariah, yaitu proses permohonan sampai putusan yang dilakukan secara sederhana, dalam kurun waktu yang terbilang cepat dalam pembacaan putusan senketa hal-hal ini juga berpengaruh terhadap biaya penyelesaian sengketa yang menjadi ringan.
Analisis Yuridis Pengakuan Dan Pemenuhan Hak-Hak Tanah Masyarakat Hukum Adat Lampung
Authors: Wardhana Yogie Kusuma , Baharudin Baharudin , Anggalana Anggalana
Published: November 2023

AbstrakSejak negara ini berdiri, sudah mengamanatkan untuk menjaga kelestarian dan eksistensi kebudayaan serta nilai dan norma yang hidup di masyarakat sebagai bagian yang tidak dapat dipisahkan dari bangsa Indonesia. Amanat ini terdapat dalam Pasal 18B ayat (2) UUD NKRI Tahun 1945 yang berbunyi: Negara mengakui dan menghormati kesatuan-kesatuan masyarakat hukum adat beserta hak-hak tradisionalnya sepanjang masih hidup dan sesuai dengan perkembangan masyarakat dan prinsip Negara Kesatuan Republik Indonesia, yang diatur dalam undang-undang. Selaras hubungan masyarakat hukum adat dengan tanah yang mereka miliki mengakibatkan lahirnya hak-hak ulayat yang dimana diatur dalam Pasal 33 ayat (3) UUD 1945. Namun, masih banyaknya konflik yang mengakibatkan masyarakat adat kehilangan tanah adatnya dan pemerintah gagal dalam menerapkan hukum untuk melindungi tanah masyarakat adat yang merupakan dampak akibat dari ketidak profesionalan pemerintah untuk menyusun norma hukum dan mengelolah sumber daya alam.
PELAKSANAAN DISPENSASI PERKAWINAN DI PENGADILAN AGAMA (PA) PANGKALAN KERINCI KABUPATEN PELALAWAN BERDASARKAN UNDANG-UNDANG NOMOR 16 TAHUN 2019 TENTANG PERUBAHAN ATAS UNDANG-UNDANG NOMOR 1 TAHUN 1974 TENTANG PERKAWINAN
Authors: Azani Muhammad , Basri Hasan , Rinaldi Aurora Putri , Nibrasullah Nibrasullah
Published: November 2023

Rumusan masalah yang Penulis rangkum dalam penelitian ini adalah bagaimanakah pelaksanaan, hambatan, dan upaya mengatasi hambatan dalam pelaksanaan Dispensasi Perkawinan di  Pengadilan Agama Pangkalan Kerinci Kabupaten Pelalawan berdasarkan Undang-Undang Nomor 16 Tahun 2019?  Tujuan dari penelitian adalah untuk  Mengetahui pelaksanaan, hambatan, dan upaya mengatasi hambatan Dispensasi Perkawinan di Pengadilan Agama Pangkalan Kerinci Kabupaten Pelalawan berdasarkan UndangUndang Nomor 16 Tahun 2019. Metode penelitian yang digunakan  adalah studi lapangan dengan jenis penelitian hukum sosiologis. Hasil penelitian diketahui bahwa Dispensasi perkawinan di Pengadilan Agama Pangkalan Kerinci Kabupaten Pelalawan merupakan permohonan dispensasi perkawinan diajukan dalam bentuk voluntair dalam arti tidak ada sengketa antara para pihak yang berperkara. Dispensasi perkawinan dari Pengadilan Agama adalah putusan yang berupa penetapan dispensasi untuk calon mempelai yang belum mencapai usia 19 tahun baik bagi pihak pria maupun pihak wanita. Secara umum karena salah satu calon baik istri atau suami usianya kurang dari 19 Tahun.  Hambatan dalam pengajuan perkara dispensasi perkawinan yang terjadi di Pengadilan Agama Pangkalan Kerinci adalah  Faktor tidak terpenuhinya kedudukan hukum para pihak dalam mengajukan perkara dispensasi perkawinan yang berakibat perkara dispensasi perkawinan yang diajukan tidak dapat dilanjutkan berdasarkan Pasal 6 Perma Nomor 5 Tahun 2019, sedangkan Faktor kurangnya alat bukti, Hakim menilai Para Pemohon tidak dapat menunjukkan bukti-bukti yang cukup untuk mendukung dalil-dalilnya mengenai keadaan yang mendesak, sehingga putusan dinyatakan ditolak. Upaya mengatasi  Hambatan Dalam Pelaksanaan Dispensasi perkawinan Di PA Pangkalan Kerinci Kabupaten Pelalawan  terkait dengan  hambatan   tidak terpenuhi berkedudukan hukum pemohon, Dalam perkara Nomor 10/Pdt.P/2020/PA.Pkc, putusan hakim akan berbunyi permohoan pemohon dinyatakan tidak dapat diterima (NO) yang itu berarti perkara tersebut mengandung cacat formil. Upaya mengatasi hambatan terhadap kurangnya alat bukti adalah  dapat memberikan kesempatan perbaikan oleh pemohon dengan mengajukan kembali permohonan baru dan melengkapi atau memperbaiki kekurangan bukti yang terjadi pada permohonan sebelumnya.
ASPEK PEMIDANAAN SEBAGAI DASAR PERTIMBANGAN HAKIM DALAM MEMUTUS PERKARA TURUT SERTA (DEELNEMING) MELAKUKAN PENGGELAPAN KENDARAAN BERMOTOR (STUDI PUTUSAN NOMOR : 100/PID.B/2022/PN.TJK)
Authors: Hartono Bambang , Anggalana Anggalana , Zhifa Ersha Nadhia
Published: November 2023

Tujuan dalam penelitian ini membahas mengenai faktor penyebab pelaku tindak pidana turut serta (deelneming) melakukan penggelapan kendaraan bermotor bagaimana pertimbangan hakim dalam memutus perkara tindak pidana turut serta (deelneming) melakukan penggelapan kendaraan bermotor berdasarkan Putusan Nomor : 100/Pid.B/2022/PN.Tjk. metode penelitian yang digunakan yaitu penelitian hukum normatif. faktor penyebab pelaku tindak pidana turut serta (deelneming) melakukan penggelapan kendaraan bermotor disebabkan karena 2 (dua) hal yaitu faktor dari dalam diri si pelaku seperti keadaan jiwa, niat, faktor kebiasaan dikarenakan dalam diri Terdakwa sudah niat jahat (mens rea) dengan menggadaikan tanpa izin sebuah 1 (satu) unit mobil Daihatsu Xenia. Sedangkan faktor lainnya adalah faktor dari luar diri si pelaku seperti, kesempatan dan lingkungan. Dimana faktor ini merupakan penyebab yang berasal dari luar diri si pelaku atau pengaruh dari luar. Pertimbangan Hakim dalam memutus perkara tindak pidana turut serta (deelneming) melakukan penggelapan kendaraan bermotor dengan mempertimbangkan fakta hukum dalam perkara, mempertimbangkan unsur-unsur pidana dalam dakwaan dan memepertimbangkan keadaan yang memberatkan dan keadaan meringankan Terdakwa selama persidangan, dimana Majelis Hakim menyatakan Terdakwa Ismail Saleh Bin Fransisco terbukti secara sah   dan meyakinkan bersalah melakukan tindak pidana turut serta melakukan Penggelapan dan menjatuhkan pidana terhadap Terdakwa Ismail Saleh Bin Fransisco oleh karena itu dengan pidana penjara selama 2 (dua) tahun dan 6 (enam) bulan.
ASPEK HUKUM TENTANG DUALISME KEDUDUKAN APARATUR SIPIL NEGARA SEBAGAI KEPALA DESA
Authors: Syafwar Robi
Published: November 2023

Penyelenggaraan Pemerintah Desa sudah berlangsung hampir sepuluh tahun sejak disahkanya UU Desa dan pemerintahpun sudah mengucurkan Dana Desa sejak tahun 2015, yang terus mengalami peningkatan. Posisi strategis dalam mengelola dana desa dalam jumlah besar mendorong minat warga desa untuk menjadi kepala desa, namun apakah setiap warga desa memiliki kesempatan yang sama untuk menjadi Kepala Desa, sebut saja seseorang PNS, apakah dapat berpartisipasi dalam pilkades, karena akan berpotensi dualisme atau rangkap jabatan jika terpilih. Fokus penelitian ini adalah: Pertama, melihat bagaimana pengaturan hukum tentang kedudukan kepala desa; Kedua, apakah ASN dapat berpartisipasi dalam pemilihan kepala desa. Tujuan dari penelitian ini adalah untuk mengetahui bagaimana pengaturan hukum tentang kedudukan kepala desa dan untuk mengetahui dapatkah ASN dapat berpartisipasi dalam pemilihan kepala desa.
PENGAWASAN TERHADAP PENGELOLAAN DAN PENGELUARAN ANGGARAN PENDAPATAN DAN BELANJA NEGARA DAN DAERAH
Authors: Santoso Jonathan , Hutapea Sintong Arion , Fitri Lezi , Kahir Suwanto
Published: November 2023

Anggaran Pendapatan Belanja Negara (APBN) dan Anggaran Pendapatan Belanja Daerah (APBD), keduanya memiliki persoalan yang krusial. Artikel ini dibuat untuk mengkaji pengawasan terhadap Pengelolaan dan Pengeluaran APBN dan APBD serta kendala dalam Pengawasan APBD dan APBN. Artikel ini disusun dengan menggunakan pendekatan normative. Pengawasan pengelolaan dan pengeluaran APBN dan APBD supaya tidak ada pihak yang memanfaatkan, menyalahgunakan pengelolaan dan pengeluaran anggaran keuangan negara atau daerah untuk kepentingan pribadi. Pengawasan lebih bersifat mencegah terjadinya pelanggaran pengelolaan dan pengeluaran anggaran keuangan negara dan daerah, kalaupun terdapat pelanggaran maka dapat segera ditemukan melalui pengawasan dan segera pula dilakukan koreksi atas kerugian. Kendala yang didapatkan dalam pengelolaan APBN dan APBD meliputi: efisiensi dan efektivitas pengelolaan dan pengeluaran tidak sesuai yang telah direncana dan ditetapkan.
PERLINDUNGAN HUKUM BAGI KORBAN KECELAKAAN LALU LINTAS YANG MENGAKIBATKAN LUKA BERAT (Studi Putusan Nomor 1166/Pid.Sus/2017/PN Bks dan Putusan Nomor : 12/Pid. Sus/2021/PN.Bks.)
Authors: Afandi Aris Nur
Published: November 2023

Upaya penindakan pelanggaran dan kejahatan lalu lintas untuk dilakukan penyidikan telah diundangkan dalam Undang-Undang Nomor 22 Tahun 2009 tentang Lalu Lintas Dan Angkutan Jalan. Penyidik Pembantu, yang dijelaskan dalam Pasal 259 ayat (2) huruf b memiliki wewenang yang sama seperti yang diatur dalam Pasal 260 ayat (1) Undang-Undang tersebut, kecuali untuk melakukan penahanan seperti yang diatur dalam Pasal 260 ayat (1) huruf h. Penahanan tersebut hanya dapat dilakukan oleh Penyidik Kepolisian Negara Republik Indonesia di bidang Lalu Lintas dan Angkutan Jalan dengan memberikan pelimpahan wewenang. Berdasarkan putusan Nomor 1166/Pid.Sus/2017/PN Bks dan Nomor 12/Pid.Sus/2021/PN.Bks, semua elemen tuntutan jaksa telah terbukti dan terpenuhi, sehingga terdakwa harus dianggap bersalah secara sah dan meyakinkan. Tidak ada alasan untuk membebaskan terdakwa dari hukuman atau tanggung jawab pidana yang dihadapinya. Oleh karena itu, terdakwa harus dinyatakan bersalah dan dijatuhi hukuman sesuai dengan peraturan undang-undang yang berlaku. Faktor-faktor yang memberatkan dan meringankan terdakwa telah dipertimbangkan dengan matang sebelum majelis hakim membuat keputusan. Dalam hal penjatuhan hukuman terhadap terdakwa dalam kasus ini, majelis hakim telah sepenuhnya menunjukkan keadilan terhadap masyarakat, negara, dan terdakwa.
LEGALITAS SISTEM AUTO PILOT KENDARAAN RODA EMPAT DI INDONESIA BERDASARKAN UNDANG-UNDANG NOMOR 22 TAHUN 2009 TENTANG LALU LINTAS DAN ANGKUTAN JALAN
Authors: Nugroho Aguk , Azis Nur , Ruminingsih Ruminingsih , Marwan Marwan
Published: November 2023

Fenomena pertumbuhan jumlah kendaraan yang tidak seimbang dengan tingkat ketersediaan infrastruktur jalan telah mendorong adanya inovasi auto pilot system oleh pabrikan kendaraan. Penelitian ini bertujuan untuk mengkaji legalitas auto pilot kendaraan roda empat di Indonesia berdasarkan Undang-Undang Nomor 22 tahun 2009 tentang lalu lintas dan angkutan jalan. Metode yang digunakan dalam pengkajian terhadap permasalahan yang diteliti dengan menggunkan pendekatan hukum positif yang berlaku di Indonesia. Hasil penelitian mendapatkan legalisasi sistem auto pilot kendaraan roda empat di Indonesia berdasarkan Undang-undang Nomor 22 Tahun 2009 tentang lalu lintas dan angkutan jalan dapat dinyatakan memenuhi karena tidak ada klausal atau pasal yang dilanggar. Adapun upaya penegakan hukum bagi pengendara yang menggunakan kendaraan berteknologi auto pilot system dapat dilakukan dengan cara, yaitu upaya preventif dan upaya represif. Keselamatan, endaraan roda empat
PEMIDANAAN TERHADAP PELAKU TINDAK PIDANA KELALAIAN MENGAKIBATKAN KECELAKAAN LALU LINTAS YANG MENGAKIBATKAN ORANG LAIN MENINGGAL DUNIA (STUDI KASUS TERHADAP PUTUSAN PENGADILAN NEGERI JAKARTA BARAT NOMOR 889/PID.B/2020/PN JKT.BRT DAN PUTUSAN PENGADILAN NEGERI JAKARTA TIMUR NOMOR 151/PID.SUS/2013/PN.JKT.TIM)
Authors: Handayani Handayani
Published: November 2023

Humam Error oleh pengemudi sepeda roda empat dan pengendara sepeda motor dalam penelitian ini adalah karena kelalaian dalam mengidentifikasi dan menginterpretasikan situasi jalan, kondisi lingkungan yang merugikan, pengetahuan mengendalikan kendaraan dalam situasi darurat yang menyebabkan kelalaian dalam membuat keputusan yang tepat dan kemudian membawanya ke keselamatan berkendara. / Tindakan mengendarai. Upaya pemidanaan terhadap pelaku tindak pidana Kelalaian Mengakibatkan Kecelakaan Lalu Lintas Yang Mengakibatkan Orang Lain Meninggal Dunia (Studi Putusan Nomor 889/Pid.B/2020/PN Jkt.Brt dan Putusan Nomor: 151/Pid.Sus/2013/PN.JKT.TIM) Majelis Hakim pada Pengadilan Negeri Jakarta Timur terhadap kasus yang menimpa M. Rasyid Amrullahrajasa yang merupakan anak Menteri Koordinator Perekonomian pada periode 2009-2014, Hatta Rajasa tidak dipengaruhi oleh status sosial dari M. Rasyid Amrullahrajasa, melainkan murni merupakan pertimbangan hukum dan sesuai dengan Konsep Restorative Justice yang mendukung asas peradilan cepat, sederhana, dan biaya ringan dan asas Ultimum Remedium. Sedangkan, Putusan terhadap kasus kecelakaan lalu lintas yang diputus oleh Pengadilan Negeri Jakarta Barat dengan nomor putusan 889/Pid.B/2020/PN Jkt.Brt atas nama terdakwa Dany Kameswara, dalam kasus kecelakaan lalu lintas yang menyebabkan korban meninggal dunia sebanyak 1 orang, Dany Kameswara diputus oleh Majelis Hakim pada Pengadilan Negeri Jakarta Barat dengan pidana penjara selama 3 ( tiga ) tahun. Dalam  putusan tersebut Majelis Hakim mempertimbangkan bahwa keluarga Dany Kameswara tidak memberikan bantuan kepada korban, bahkan Dany Kameswara tidak menunjukan rasa penyesalan atas perbuatannya. Oleh karena itu, Hakim Negeri Jakarta Timur dalam Putusan Nomor 151/Pid.Sus/2013/PN.JKT.TIM menjatuhkan pidana penangguhan selama 6 (enam) bulan atas nama terdakwa M. RASYID AMRULLAHRAJASA,mengingat M. RASYID AMRULLAHRAJASA Tujuan pemidanaan yang ditopang oleh teori keadilan restoratif adalah upaya untuk mengembalikan keseimbangan korban.
PERTANGGUNGJAWABAN PIDANA PELAKU TINDAK PIDANA PENCABULAN SESAMA JENIS DALAM KUHP NASIONAL
Authors: Febriani Lola , Isnawati Muridah
Published: November 2023

Perbuatan cabul dewasa ini menjadi perhatian khusus dikarenakan perbuatan tersebut termasuk kejahatan seksual yang cukup meresahkan di masyarakat kita, sehingga mengakibatkan aktivitas seksual sesama jenis ini menyimpang dari yang seharusnya Perilaku cabul yang dilakukan antar orang dewasa sesama jenis telah diatur di KUHP yang bisa menjadi acuan agar tidak melakukan perbuatan tersebut. Tujuan penelitian ini adalah untuk mengetahui pertanggungjawaban pelaku tindak pidana pencabulan sesama jenis dalam KUHP nasional dan untuk mengetahui akibat hukum bagi pelaku tindak pidana pencabulan sesama jenis dalam KUHP nasional. Penelitian ini menggunakan penelitian hukum normatif dengan pendekatan perundang-undangan (statue approach).  Hasil penelitian ini adalah pertama, bahwa pertanggungjawaban pelaku tindak pidana pencabulan sesama jenis diatur di Pasal 414 ayat (1) dan ayat (2) Undang-Undang Nomor 1 Tahun 2023 tentang Kitab Undang-Undang Hukum Pidana. Kedua, akibat hukum bagi pelaku tindak pidana pencabulan sesame jenis diatur di Pasal 414 ayat (1) dan ayat (2)
Study on Antioxidant Activity of Spearmint Essential Oil and Pure Dew
Authors: Han Li , Gao Yan , Guo Yingkai , Ma Huimin , Jian Xuehui
Published: November 2023

Spearmint essential oil and pure dew were used as research objects, the antioxidant capacity of spearmint was evaluated by measuring the scavenging capacity of superoxide anion radical and hydroxyl radical, providing technical support for the subsequent development and utilization of spearmint truffle and essential oil. The results showed that when the volume fraction (V/V) of spearmint essential oil was 1%, its antioxidant capacity was the strongest, and its scavenging rates of superoxide anion radical and hydroxyl radical were 50.94% and 90.11% respectively; When the volume fraction (V/V) of spearmint hydrosol was 100%, its antioxidant capacity was the strongest, and its scavenging rates of superoxide anion radical and hydroxyl radical were 47.65% and 45.60%.
Late-Stage Peptide Modification Via Aspartic Acid as Endogenous Directing Group
Authors: Liao Mengjie , Yao Bo
Published: November 2023

Polypeptide drugs have become one of the most crucial tools in drug research and other related fields because of their high pharmacological activity, low dosage, low toxicity and side effects. However, the problems of poor metabolic stability, short half-value period and difficulty in penetration have greatly limit the development of new polypeptide drugs rendering the functional modification of peptides a crucial tool to polypeptide drugs. In this paper, we developed a new strategy for peptide functionalization through the usage of aspartic acid side chain as an endogenous directing group to realize polypeptide’s selective bond arylation.
Expression and Purification of SMGL-1
Authors: Wang Mengya , Mei Kunrong
Published: November 2023

Recombinant protein expression is a crucial technique in biology, with E. coli being the most widely used expression system. However, due to growth pressure, the expression of large molecular weight proteins in E. coli has remained a challenging task. SMGL-1, a newly discovered protein in C. elegans with Rabin 8 function, plays an important role in biology. To better understand the function of SMGL-1, we first predicted and analyzed its protein structure and properties using artificial intelligence. We then conducted studies on its expression and purification. Through optimization of IPTG concentration and expression strains, we successfully expressed SMGL-1 in E. coli, providing guidance for the expression of large proteins in E. coli. Furthermore, we explored the purification of SMGL-1 using GST affinity chromatography, Nickel affinity chromatography, and ammonium sulfate precipitation methods, laying the foundation for future purification work on SMGL-1.
An Analysis of Honeybee Population Dynamics
Authors: Yang Yicheng , Lubin Mia , Zhou Ziyao , Niu Kevin
Published: November 2023

Colony Collapse disorder (the CCD) is the term used to describe the global decline in bee populations. The research mission of this article is to identify which factors contribute to the CCD and understand how these factors contribute to the decline of bee populations, which may provide methods for restoring global bee populations. Two parts of the study will be mentioned in this article. The first half of our study was to understand such collective intelligence (and habits such as seasonal behavioral change) and use a mathematical model to simulate it. We then input the variables that we used to simulate honeybee collective intelligence into a time-dependent model to predict the population of a honey colony over time. In this model, we excluded the factors that might cause the CCD on purpose, so we could use it as a controlled set of honeybee natural population dynamics. We compared the results of this population model to experimental data we found, and they matched within certain degrees. The second half of our study was to perform a sensitivity analysis by introducing back the three factors that might cause the CCD to the population model including climate change, pesticides, and habitat destruction. The paper further discussed the strength and weaknesses of the mathematical model and used this model to predict how many honeybee hives were needed to support the pollination of a 20-acre parcel of land containing crops that benefit from pollination. Additionally, an infographic of our method was illustrated.
Effect of Vibration and Tilting Mechanical Loading Device on Parthenogenetic Embryos Development of Mice Immature Oocytes
Authors: Liu Qinli , Bo Huo
Published: November 2023

The growth and development of early mammalian embryos mainly take place in the fallopian tube, which not only provides nutrients for embryonic growth and development but also offers suitable mechanical conditions. The embryo culture system established in assisted reproductive technology mainly simulates the environment in which oocytes and embryos grow and develop in vivo. However, current in vitro embryo culture is mainly static and cannot completely mimic the mechanical environment in which embryos grow and develop in vivo. Therefore, to more accurately simulate the mechanical environment of embryos in the fallopian tube, we have developed a dynamic culture device to investigate the effects of mechanical stimulation on the in vitro maturation of immature oocytes and their parthenogenetic developmental potential. Immature mice oocytes were subjected to in vitro maturation by static culture and vibration (3 Hz, 6 Hz) with tilting for 15∼16 hours. The maturation of oocytes was observed after the culture period. The mature oocytes were activated by parthenogenesis and the rate of embryo compaction and formation of parthenogenetic blastocysts was analyzed. The results showed that using 3 Hz vibration and tilting can significantly improve the parthenogenetic development potential of immature mice oocytes.
Improvement of Oleanolic Acid Production in Saccharomyces Cerevisiae Based on OptKnock Framework
Authors: Li Xiaohan , Hu Bing
Published: November 2023

Biosynthesis of plant-derived natural products in the eukaryotic microbe Saccharomyces cerevisiae often faces the issue of the inefficient production due to the poor compatibility between the heterologous genes and chassis cells. In order to improve the biosynthetic efficiency of heterologous production of plant secondary metabolites in S. cerevisiae, people usually do metabolic engineering in and around the heterologous metabolic pathways based on researchers’ experience and mass of trials, which usually consumes a lot of manpower and financial resources. Herein, to further improve the heterologous production of oleanolic acid (OA), a pentacyclic triterpenoid in many plants with several promising pharmacological activities, in a genetically engineered, OA-producing strain S. cerevisiae OA07 effectively, a genome-scale metabolic model of the strain was developed, with the named as Yeast-OA07, and then OptKnock, a flux balance analysis-based pathway design algorithm with bilevel objectives, was utilized to develop in silico gene-knockout strategies to guide the molecular operations in S. cerevisiae OA07. Yeast8-OA07 contained 1133 genes, 2702 metabolites, and 3997 reactions. Five in silico gene-knockout strategies, which were expected to increase OA productivities, were obtained based on the metabolic flux analysis of Yeast8-OA07 through OptKnock. Afterwards, five mutant strains, named as LK1, LK2, LK3, LK4 and LK5, were constructed according to the in silico strategies. It was found that the mutant strain LK2, in which 2-amino-4-hydroxy-6-hydroxymethyl dihydropteridine diphosphokinase-encoding gene FOL1 and formate dehydrogenase-encoding gene FDH1 were deleted, had an OA yield of 125.04 mg·L-1, which was significantlyhigher than the original strain OA07 (89.50 mg·L-1), while the mutant strain LK5, which eliminated paminobenzoic acid synthase-encoding gene ABZ1 and glycine hydroxymethyl transferase-encoding gene SHM1, had an even higher OA yield of 207.37 mg·L-1. Nevertheless, strain LK6, which was developed by integrating the in silico gene-knockout strategies of LK2 and LK5, had a significant decrease of OA production than S. cerevisiae OA07, indicating that in silico knockout strategies do not fit to in vivo iteration directly. Our study provides a novel, efficient method to improve the heterologous production of plant metabolites in microbial cell factories.
The Purification of Human Carboxypeptidase O
Authors: Zhang Xinjie
Published: November 2023

In mammals, a limited number of proteases catalyze with acidic amino acids as substrates. At present, there are only three known proteases: CCPs, carboxypeptidase O (CPO), and aspartate acylase (ASPA). Human CPO is a digestive enzyme that prefers glutamate as a substrate. It locates to the apical membrane of intestinal epithelial and is glycosylated protein. CPO is difficult to purify as it is a GPI-anchored protein. To obtain purified CPO, a truncated form called hCPOΔC was designed, which removed the C-terminal sequence of hCPO and was followed by His tag. Firstly, the truncated variant hCPOΔC (residues 1-349) was cloned into pFastBac vector to construct bacmid. Then the verified bacmid was transfected into Sf9 cells for expression. After the protein was successfully expressed, cell medium was collected and incubated with Ni resins. The target protein was eluted by imidazole through affinity chromatography. A purification method of human CPO with deglutamylation activity was successfully established using insect cells expression system. Purified hCPOΔC could hydrolyze glutamate in polypeptides.
Effect of Grape Seed Procyanidins Combined with Allicin on Lipid Levels in Hyperlipidemic Rats
Authors: Sun Liu-Qing , Zhu Chao-Feng , He Jiang-Qin
Published: November 2023

Objectives: To study the effects of grape seed proanthocyanidins (GSP) combined with allicin on serum lipids level and vascular damage in a rat model of hyperlipidemia. Materials and methods: SD rats(male, 170-220 gn= 40) were randomized into five groups (n = 8/group): modelhigh fat and cholesterol diet; controlnormal diet; model+low-dose (GSP+allicin )(GSP 45mg/kg, allicin 30mg/kg, orally); model+high-dose (GSP+allicin) (GSP180mg/kg, allicin 90mg/kg, orally) and positive control (model+simvastatin (4 mg/kg)). Normal control group was fed conventionally, and remaining four groups were fed high cholesterol and fat food to replicate the high fat model. After 9 weeks, the normal control group continued to receive regular feeding, while the other groups continued to receive high-fat feeding. At the same time, model and normal control groups were given equal volume of physiological saline by gavage, and the other treatment groups began to receive corresponding drugs by gavage once a day. After 4 weeks, serum levels of total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) as well as high-density lipoprotein cholesterol (HDL-C) in rats were determined. And the body weight of rat, total antioxidant capacity (T-AOC), superoxide dismutase (SOD) and malondialdehyde (MDA)in serum were identified. The level of endothelin-1(ET-1) was quantitative analysis by ELISA assay. Results: In comparison to normal controls, the model group displayed a marked rise in body weight, an increment in serum concentrations of LDL-C, TG and TC, as well as a decline in HDL (P<0.01), demonstrating successful model replication; All doses of GSP in combination with allicin resulted in a reduction in TG, LDL-C, and TC and an enhancement in HDL-C in contrast to the model control (all P<0.05). High-dose (GSP+allicin ) decreased MDA, and increased T-AOC and SOD activity(all P<0.01). All doses of GSP combined with allicin decreased ET-1 (all P<0.05). In addition, the protective effect of GSP combined with allicin was dose-dependent. Conclusions: Studies have shown that GSP combined with allicin can significantly improve blood lipids in hyperlipidemic rats, and this mechanism may be related to antioxidants and reduced endothelial damage.
Exploring the Effectiveness of Multi-Objective Training for Organ Substructure Segmentation in Medical Imaging
Authors: Duan Wenxin , Guo Yingwei , Yang Yingjian , Zeng Nanrong , Wang Shicong , Xu Jiaxuan , Chen Rongchang , Kang Yan
Published: November 2023

Chronic obstructive pulmonary disease (COPD) is closely related to the right ventricle and lung lobes. This study focuses on the segmentation of the right ventricle and lung lobes. We conducted experiments using the MMWHS and our lung lobe datasets and evaluated the segmentation using different training models. We observed that the multi-objective segmentation approach has advantages over single-objective segmentation in segmenting the right ventricle and lung lobes. For the segmentation of the right ventricle, the multi-objective segmentation approach yielded an improvement of 2.0% in the Dice coefficient and 2.5% in the Jaccard index compared to single-objective segmentation. For the segmentation of five lung lobes, the multi-objective segmentation outperformed the single-objective segmentation with Dice coefficient improvements of 1.4%, 1.0%, 1.5%, 0.7%, and 1.3%, respectively.
Development of Colloidal Gold Test Strips for the Detection of Oxamyl Residues in Tobacco
Authors: Wang Chunqiong , Zhang Ke , Cai Yongzhan , Zhang Xiaowei , Long Jie , Gu Jianlong , Bu Yunhong , Sun Haowei , Li Dan , Ding Yishu , Cai Jieyun , Chen Dan
Published: November 2023

In this study, monoclonal antibodies against oxamyl were prepared, and colloidal gold immunochromatography was used to design a rapid test strip product for the detection of oxamyl in tobacco with high specificity, accuracy and stability without cross-reactivity to commonly used tobacco fungicides based on the optimization of conditions such as pH value of diluent, diluent dosage, concentration of antibody marker, type of confining solution and complex solution. 5 The results of five samples of post-harvest ready-to-bake tobacco and first-harvest tobacco were consistent with the gas chromatographic method, which proved the reliability of the test strips. The limits of detection for the post-harvest and first-harvest tobacco samples were 0.1 mg/kg, and the test strips developed in this study are suitable for mass testing in tobacco laboratories with good application prospects because of their short detection time, simple pre-treatment and detection methods.
Spectrophotometric Determination of Cysteine Hydrochloride Content by Ferric Chloride-Potassium Ferricyanide
Authors: Tu Changqing , Wen Xinrong
Published: November 2023

In the acidic medium, hydrosulfuryl(-SH) in cysteine hydrochloride can reduce Fe3+ to Fe2+, then Fe2+ react with potassium ferricyanide to form KFe[Fe(CN)6](soluble Prussian blue). Prussian blue has a maximum absorption at 727 nm, Bill’s law is observed between mass concentration of cysteine hydrochloride and absorbance of Prussian blue, the content of cysteine hydrochloride is indirectly determinated by measuring the absorbance of Prussian blue. An accurate, simple, fast spectrophotometric method for the determination of cysteine hydrochloride content by ferric chloride-potassium ferricyanide has been established. The optimal determination conditions of cysteine hydrochloride content are explored. The cysteine hydrochloride content is determinate by this method.
Bioinformatic Analysis of the Mechanism of Action of COL3A1 in Pan-Cancer and Design of Specific Inhibitors for Its Conformation
Authors: Cao Xinyang , Zhang Yu , Jin Fanxiang , Li Shufang , Zhang Wenbiao , Mei Chen , Yin Dan
Published: November 2023

In this study, we investigated the mechanism of action of COL3A1 in various types of cancers by bioinformatics analysis and designed some specific inhibitors aimed at the treatment of this gene. We found that COL3A1 was highly expressed in several cancer types and correlated with tumor progression and prognosis. Through systems biology analysis, we identified a central role for COL3A1 in cancer development, including cell proliferation, metastasis and invasion. We also used molecular dynamics simulations and drug screening techniques to design anticancer drugs with potential COL3A1 inhibitory functions. These results provide a strong rationale for the development and use of COL3A1 as a therapeutic target.
Polysiloxane in the Biomedical Field: Bibliometric and Visual Analysis
Authors: Chen Ting , Li Jing , He Qingjiao , Huang Bin
Published: November 2023

Polysiloxane is widely used in biomedical applications because of its good biocompatibility and excellent micro and nano-scale processability. Using PubMed and Citex’s data analysis platform, this paper examines polysiloxane research and compares its development history from 2002-01 to 2021-12. Research hotspots, keyword analysis, and the number of papers published on polysiloxane in biomedicine are used in this study to analyze and discuss the current status of polysiloxane research in biomedicine. Over the last twenty years, there has been a significant surge in the number of publications related to the use of polysiloxane in biomedicine. This trend highlights the growing intersection and collaboration between the fields of polymeric material science and biomedical disciplines. Therefore, statistics about the number of papers on polysiloxane and the frequency of hot words are valuable resources for biomedical research and development.
Effect of Mechanical Stimulations on the Cytoskeleton of Mouse Oocytes During Maturation
Authors: Liu Qinli , Zhao Sen , Bo Huo
Published: November 2023

In the reproductive system of female mammals, the early embryos grow and develop in the fallopian tube, where they are stimulated by fluid flow and ciliary vibration. The mechanical environment of the fallopian tube affects the development of embryos. This study is focused on the role of mechanical stimulation on the cytoskeleton of oocytes during oocyte maturation in vitro. The 3 Hz microvibration and tilting stimulations were applied to mouse immature oocytes. The oocyte maturation rate and area of the first polar body under dynamic stimulation were compared with those of the static culture group. A fluorescence assay was used to study the localization of the cytoskeleton during oocyte maturation. The results indicated that mechanical stimulation can change the localization of F-actin and spindle, and oocytes discharged smaller areas of the first polar body.
Screening and Bioinformatics Analysis of Differential Genes in Autism Spectrum Disorder Based on GEO Database
Authors: Zhu Jinyi , Meng Haoran , Li Yan
Published: November 2023

Objective: The prevalence of autism spectrum disorder (ASD) in children has been increasing year by year, which has seriously affected the quality of life of children. There are many theories about the cause of ASDs, with some studies suggesting that it may be related to gene expression levels or inflammation and immune system dysfunction. But the exact mechanism is not fully understood. Methods: profile of gene expression The protein interaction network (PPI) of differentially expressed genes was created using the STRING web tool and GSE77103, which was chosen from the gene expression omnibus (GEO) database. Using the CytoHubba plugin of Cytoscape program, the hub genes were examined. The hub gene regulatory network for miRNA-mRNA was then built. Results: We identified 551 differentially expressed genes(DEGs) in 8 children with ASD and normal children. In addition, we screened out 10 hub genes (MX1, ISG15, IRF7, DDX58, IFIT1, BCL2L1, HPGDS, CTSD, PTGS2 and CD68) that were most associated with the development of ASDs. Then, microRNAs (miRNAs) closely related to hub genes (such as has-miR-27a-5p) were screened, and the miRNA-mRNA regulatory network was constructed. Conclusion: In this study, a total of 10 hub genes were identified, including MX1, ISG15, IRF7, DDX58, IFIT1, BCL2L1, HPGDS, CTSD, PTGS2 and CD68, which are closely related to ASD. These genes may play a key role in the occurrence and progression of ASD. In addition, we also revealed some miRNAs that regulate the hub genes of ASD. These results may deepen our understanding of ASD and provide potential biomarkers and targets for future treatment of patients with ASD.
Research on Brain Signals Classification Based on Deep Learning
Authors: Tian Chenfei
Published: November 2023

With the continuous expansion of brain-computer communication, the precise identification of brain signals has become an essential task for brain-computer equipment. However, existing classification methods are primarily concentrated on the extraction features of brain signals and obtain unacceptable performance when directly used the model to a new brain signals data, which is caused by the different people has extraordinary brain signals. In this work, we utilize the deep learning methods not only extract the features of brain signals but also learn the order information of brain signals, which can satisfy the universal brain signals. Indeed, we utilize the classification features dimension distance loss function to optimize the proposed model and enhance the classification accuracy and we compare our model with existing classification methods to evaluate proposed model. From our extensive experimental results and analysis, we can conclude that our model can achieve the classification of brain signals with the reasonable accuracy and acceptable costs.
Suppression or Inhibition of VEGFs on Splenic Angiosarcoma Cell with Traditional Chinese Medicine Zhi Gan Cao
Authors: Zhao Tianyang
Published: November 2023

Primary splenic angiosarcoma is a very rare disease that causes the development of malignant tumors in the vascular endothelium of the splenic sinuses. Moreover, the disease maintains a very low survival rate for patients to live over 5 years, which is relatively low when compared to another splenic cancer, splenic lymphomas. The treatment options for splenic angiosarcoma narrow down to surgical removal or radiation combined with chemotherapy, but both cost a lot, so discovering potential alternative treatments may eventually increase the possible survival rate. Ginseng and Zhi Gan Cao are both common herbs in Traditional Chinese Medicine (TCM); however, the price of Ginseng is much higher than that of Zhi Gan Cao. A possible reason could be the frequent studies and researches over Ginseng’s active ingredient, ginsenoside rh2 or rg3 as they are both potent cancer treatments. The reason to study Zhi Gan Cao and predict its possible potential in cancer treatment is due to the similarity between its active ingredient and the active ingredient in Ginseng, namely, ginsenoside rh2 and licorice saponins. Both TCM contain the active ingredient, triterpenoid saponin, as their main composition, and the further text will predict the possible research and results that may be taken in vitro to reveal the question of whether licorice saponin has the potential to become a major treatment for splenic angiosarcoma or not.
Exploration on Anti-Depression Mechanism of Baihe Zhimu Decoction Based on Network Pharmacology and Molecular Docking
Authors: Zhang Xianbin , Wang Caihong
Published: November 2023

Objective: To analyze anti-depression mechanism of Baihe Zhimu decoction (BZD) based on network pharmacology method, which provides reference for the development of new drugs and the clinical application of classical prescriptions. Method: The main chemical components and targets of Baihe and Zhimu were obtained through traditional Chinese medicine pharmacology system technology platform (TCMSP) database, and the active components of TCM were filtered according to ADME; Major targets for anti-depression were get through Gencards, OMIM and DRUGBANK databases; Protein interaction analysis was performed using the String platform; Build PPI networks and mine potential protein functional modules in the network; The Metascape platform was used to analyze the “drug-ingredients-target” and its involved biological processes and pathways; Finally, the molecular docking validation was performed by Systems Dock Web Site. Results: The core active ingredients of BZD treating depression are kaempferol and Stigmasterol, The core targets are AKT1, TNF, TP53, PTGS2, and CASP3. The biological pathway of the anti-depression mainly acts on Lipid and atherosclerosis, Chemical carcinogenesis and receptor activation. Molecular docking results showed that AKT1, TNF and TP53 have good affinity with components kaempferol and Stigmasterol. Conclusion: This study initially revealed the mechanism of multicomponent, multiple target and multiple pathway of anti-depression, which may be related to neuroactive ligand-receptor interaction, atherosclerotic, PI3K-Akt and TNF signaling pathway.
Study on Calibration of Extracorporeal Pacemaker
Authors: Ren Tingting , Fang Meina , Luo Han , Zeng Lingjian , Zeng Cheng
Published: November 2023

Extracorporeal pacemaker is cardiac rhythm management device with non-implantable pulse generator and is widely used medical institutions. Parameters such as pulse duration, pulse amplitude, pulse rate, sensibility, and PVARP can directly decide the metrological performance of the instrument. However, at present, there is no relevant calibration specification for extracorporeal pacemaker in China to calibrate the important parameters. This article presents a novel calibration method for extracorporeal pacemaker by determining corresponding environmental conditions, calibration standards, and calculation equations. The calibration results of the important parameters can meet the requirements of GB 16174.2-2015 Implants for surgery – Active implantable medical devices – Part 2 Cardiac pacemakers, which shows that the calibration method is scientific and practical for metrological performance evaluation of extracorporeal pacemaker.
The Effect of Oxaliplatin on the Immunogenic Cell Death and Cell Apoptosis of Human Merkel Cell Cancerous Tumor
Authors: Zhang Boyang
Published: November 2023

Oxaliplatin, as previously studied in the paper, is a derivative of Cisplatin that is effective in treating the Lewis Lung Carcinoma (LLC)4. As it can actively induce immunogenic cell death of the cancer cells, and result in apoptosis, which increases the therapeutic efficacy in the LLC cancer treatment.4 Merkel cell caner is a type of skin cancer that is rare but highly aggressive, with high metastasizing and reoccurring rate. In this study, we aim the determine the potential of Oxaliplatin to induce apoptosis and ICD in cancerous Merkel cell line MCC1, in associate with the PD-1 inhibitor Nivolumab. The cancer cells will be treated with Oxaliplatin at concentrations 1 mM, 10 mM, or 100 mM. Avelumab and PBS will be used as the positive and negative control, respectively. The treated cells will be measured by checking for tumor size change in confocal microscopy and MTT assay, measuring the ICD using flow cytometry analysis of CRT expression, and conducting Western Blot for Cytokeratin 20 expression. The results of the study will provide insights on the potential of Oxaliplatin as a treatment of Merkel Cell Cancer in the future.
Analysis and Prediction of Significant Genes in Gastric Cancer Based on TCGA Database
Authors: Li Ruisong , Lu Yue , Yi Zhaoyi , An Juan
Published: November 2023

Objective: Use TCGA data to screen the significantly expressed genes in gastric cancer,then analyze the biological functions of characteristic genes.To identify potential targets for the diagnosis, prognosis and therapeutic monitoring of gastric cancer. Methods: Download the high-throughput gastric cancer patient tissue mRNA expression data from the TCGA database, and used the edgeR software package to perform gene differences using the Rstudio software according to the |logFC|>1 and p<0.05 standards analysis, then performed enrichment analysis and constructed protein interaction network of the differently expressed genes to find the significant genes.Finally,analyzed the relationship between significant genes and gastric cancer patient prognosis. Results: The RNA-seq expression data of 375 cases of gastric cancer and 32 adjacent tissues were obtained from the TCGA database. The differential analysis yielded 4320 differential genes, of which 2718 were highly expressed and 1602 were low expressed. Through enrichment analysis, it is found that the differential genes are mainly related to the neural active ligand-receptor interaction, calcium signaling pathway, Protein digestion and absorption, chemical carcinogenesis, and cytochrome P450 metabolism of foreign compounds. Further survival analysis screened out the five significant genes of ALB, AFP, IGFBP1, APOH, and TF are related to the prognosis of gastric cancer. Conclusion: The five significant genes screened by TCGA data may be related to the prognosis of gastric cancer and are expected to become prognostic biomarkers.
Single Cell Sequencing Analysis for Blood Tissue
Authors: Zhu Sijia
Published: November 2023

Single-cell sequencing is a technique based on sequencing genomic, transcriptomic and epigenomic analysis at the level of individual cells. Compared with traditional sequencing techniques, it can study intercellular heterogeneity and provide in-depth information on cell distribution, cell types and cell function of a specific population. This paper used blood tissue samples to analyze single-cell sequencing. Cells were downscaled by principal component analysis (PCA) and used Leiden clustering to discover cell heterogeneity in the same cluster and annotated after screening marker genes. Finally, 13 marker genes MTRNR2L8, NKG7, FLNA, IL7R, CCR7, CD8B, MKI67, LGALS3, TYROBP, C0251_TotalSeqC, CD79A, HLA-C, CCL4, and MCM5 in the experimental samples were found by cellular enrichment, which are strongly associated with white stuffy syndrome, skin damage, giant cell virus infection and other diseases are closely linked. In the medical field, the experimental results can be used to study the molecular characteristics and functional differences of different cell types under physiological and pathological conditions, thus providing insight into the pathogenesis and treatment of diseases such as leukoaraiosis syndrome, skin damage, and cytomegalovirus infection.
The Portfolio of Evidence for Lab-To-Clinic Innovative Product Bio-Printed Cartilage with Living Cells
Authors: Zhu Yi
Published: November 2023

Bio-printed cartilage with living cells is a regenerative cartilage scaffold that has living cells 3D printing out for the treatment of osteoarthritis and other conditions that damage articular cartilage. It is a new type of scaffold that is biocompatible, biomimetic, regenerative, and long term in use. Currently, there is a clinical and market lack of products for the regeneration of therapeutic cartilage, so the need for such scaffolds is necessary. Bio-printed cartilage with living cells from concept to product development and non-clinical and clinical testing to market launch requires evaluation of the scaffold for safety, efficacy, and compliance with specific requirements of the medical directives regulation and combined advanced therapy medicinal products.
Research Progress on the Relationship Between Sleep Status During Pregnancy and Gestational Diabetes Mellitus
Authors: Chen Huanjun , Yan Shijiao , Song Xingyue , Lv Chuanzhu
Published: November 2023

In recent years, the morbidity of gestational diabetes mellitus (GDM) maintained an upward trend. GDM can’t merely lead to negative pregnancy consequences, but also endanger life of the mother and child in severe cases, and may also bring long-term complications and sequelae to the mother and child. Studies using subjective or objective methods to evaluate the correlation between sleep and GDM are inconsistent, but still suggest that poor sleep status may be related to GDM. In order to further understand the current research status of the relationship between sleep measured by different tools and gestational diabetes mellitus, this paper will review the articles from Web of Science, Wanfang, PubMed, VIP, CNKI and other databases.