bluuebunny/crossref_metadata_2025 huggingface dataset:
Microstructure Refinement of 301 Stainless Steel via Thermomechanical Processing
Authors: Al-Fadhalah Khaled J. , Al-Attal Yousif , Rafeeq Muhammad A.
Published: October 2022

The current study applied thermomechanical processing (TMP) on 301 austenitic stainless steel to produce an ultrafine-grained austenitic structure, examining the dual effects of deformation at subzero temperature and TMP cycles on the strain-induced α′-martensitic transformation and austenite reversion occurring upon subsequent annealing. Three TMP schemes were adopted: (1) one cycle using a strain of 0.30, (2) two cycles using a strain of 0.20, and (3) three cycles using a strain of 0.15. Each cycle consisted of tensile deformation at −50 °C followed by annealing at 850 °C for 5 min. Compared to other schemes, the use of three cycles of the 0.15 strain scheme resulted in a significant formation of the martensitic phase to about 99 vol.%. Consequently, the austenite reversion occurred strongly, providing a mixture of the austenitic structure of reverted ultra-fine grains and retained coarse grains with an average grain size of 1.9 µm. The development of a mixed austenitic structure was found to lower the austenite stability and thus enhance the α′-martensitic transformation upon deformation in subsequent cycles. Moderate growth of high-angle grain boundaries occurred in the austenitic phase for all schemes, reaching a maximum of 64% in cycle 3 of the 0.15 strain scheme. The tensile behavior during subzero deformation was generally characterized by an initial strain hardening by slip (stage I), followed by a remarkable increase in strain hardening rate due to the strain-induced α′-martensitic transformation (stage II). Further straining promoted breakage of the α′-martensite banded lath structure for forming dislocation cell-type martensite, which was marked by a decline in strain hardening rate (stage III). Accordingly, the latter hardening stage had a lesser hardness enhancement of deformed samples with an increasing number of cycles. Nevertheless, the yield strength for samples processed by the 0.15 strain scheme improved from 450 MPa in cycle 1 to 515 MPa in cycle 3.
Effects of Gestational Diabetes Mellitus on Cholesterol Metabolism in Women with High-Risk Pregnancies: Possible Implications for Neonatal Outcome
Authors: Zeljković Aleksandra , Ardalić Daniela , Vekić Jelena , Antonić Tamara , Vladimirov Sandra , Rizzo Manfredi , Gojković Tamara , Ivanišević Jasmina , Mihajlović Marija , Vujčić Sanja , Cabunac Petar , Spasojević-Kalimanovska Vesna , Miković Željko , Stefanović Aleksandra
Published: October 2022

Metabolic disorders in pregnancy, particularly gestational diabetes mellitus (GDM), are associated with an increased risk for adverse pregnancy outcome and long-term cardiometabolic health of mother and child. This study analyzed changes of serum cholesterol synthesis and absorption markers during the course of high-risk pregnancies, with respect to the development of GDM. Possible associations of maternal lipid biomarkers with neonatal characteristics were also investigated. The study included 63 women with high risk for development of pregnancy complications. Size and proportions of small low-density (LDL) and high-density lipoprotein (HDL) particles were assessed across trimesters (T1–T3), as well as concentrations of cholesterol synthesis (lathosterol, desmosterol) and absorption markers (campesterol, β-sitosterol). During the study, 15 women developed GDM, while 48 had no complications (non-GDM). As compared to the non-GDM group, women with GDM had significantly higher triglycerides in each trimester, while having a lower HDL-C level in T3. In addition, they had significantly lower levels of β-sitosterol in T3 (p < 0.05). Cholesterol synthesis markers increased across trimesters in both groups. A decrease in serum β-sitosterol levels during the course of pregnancies affected by GDM was observed. The prevalence of small-sized HDL decreased in non-GDM, while in the GDM group remained unchanged across trimesters. Newborn’s size in the non-GDM group was significantly higher (p < 0.01) and inversely associated with proportions of both small, dense LDL and HDL particles (p < 0.05) in maternal plasma in T1. In conclusion, high-risk pregnancies affected by GDM are characterized by altered cholesterol absorption and HDL maturation. Advanced lipid testing may indicate disturbed lipid homeostasis in GDM.
Brucella Phagocytosis Mediated by Pathogen-Host Interactions and Their Intracellular Survival
Authors: Huy Tran X. N. , Nguyen Trang T. , Kim Heejin , Reyes Alisha W. B. , Kim Suk
Published: October 2022

The Brucella species is the causative agent of brucellosis in humans and animals. So far, brucellosis has caused considerable economic losses and serious public health threats. Furthermore, Brucella is classified as a category B bioterrorism agent. Although the mortality of brucellosis is low, the pathogens are persistent in mammalian hosts and result in chronic infection. Brucella is a facultative intracellular bacterium; hence, it has to invade different professional and non-professional phagocytes through the host phagocytosis mechanism to establish its lifecycle. The phagocytosis of Brucella into the host cells undergoes several phases including Brucella detection, formation of Brucella-containing vacuoles, and Brucella survival via intracellular growth or being killed by host-specific bactericidal activities. Different host surface receptors contribute effectively to recognize Brucella including non-opsonic receptors (toll-like receptors and scavenger receptor A) or opsonic receptors (Fc receptors and complement system receptors). Brucella lacks classical virulence factors such as exotoxin, spores, cytolysins, exoenzymes, virulence plasmid, and capsules. However, once internalized, Brucella expresses various virulence factors to avoid phagolysosome fusion, bypass harsh environments, and establish a replicative niche. This review provides general and updated information regarding Brucella phagocytosis mediated by pathogen-host interactions and their intracellular survival in host cells.
Mechanical Properties and Failure Mechanism of the Weakly Cemented Overburden in Deep Mining
Authors: Zhang Guojian , Guo Guangli , Wei Wei , Wang Jun , Li Huaizhan , Du Qiu
Published: October 2022

With increases in the mining depth and area in the Ordos coal field, the failure law of the super thick sandstone in the Zhidan group leads to frequent disasters, such as rock bursts and mine earthquakes, which have become a significant issue, restricting large-scale continuous mining. To adequately understand the movement mechanism of the super-thick and weakly cemented overburden, and to promote the large-scale mining of the coal resources under it, this study analyzes the physical and mechanical properties, along with the microstructural characteristics, of the weakly cemented overburden of the Yingpanhao Coal Mine through mechanics tests, scanning electron microscope tests (SEM) and hydrolysis experiments. A two-dimensional discrete element model of the survey region is then built to explore the temporal and spatial evolution laws of the overburden failure. The results show that, even though poorly cemented strata such as the Cretaceous Zhidan group sandstone and the Zhiluo group sandstone are weak in lithology, their unique mineral composition and microstructural characteristics give them a greater rigidity when their thickness reaches a certain value. The surface subsidence exhibits a sudden increase, and the dynamic disaster range of the overlying strata is wide when deep multi-face mining was carried out under the super-thick and weakly cemented overburden. The temporal and spatial evolution laws of the strata subsidence and influence boundary are closely related to their depth, and their relationships evolve into the Boltzmann function and Boltzmann–parabolic function, respectively. The failure mode of the super-thick and weakly cemented overburden is ‘beam–arch shell–half arch shell’, and the failure boundary exhibits arch fractures.
Remediation of Cr(VI)-Contaminated Soil by Biochar-Supported Nanoscale Zero-Valent Iron and the Consequences for Indigenous Microbial Communities
Authors: Yang Jianwei , Tan Xiangpeng , Shaaban Muhammad , Cai Yajun , Wang Buyun , Peng Qi’an
Published: October 2022

Biochar/nano-zero-valent iron (BC-nZVI) composites are currently of great interest as an efficient remediation material for contaminated soil, but their potential to remediate Cr-contaminated soils and effect on soil microecology is unclear. The purpose of this study was to investigate the effect of BC-nZVI composites on the removal of Cr(VI) from soil, and indigenous microbial diversity and community composition. The results showed that after 15 days of remediation with 10 g/kg of BC-nZVI, 86.55% of Cr(VI) was removed from the soil. The remediation of the Cr-contaminated soil with BC-nZVI resulted in a significant increase in OTUs and α-diversity index, and even a significant increase in the abundance and diversity of indigenous bacteria and unique bacterial species in the community by reducing the toxic concentration of Cr, changing soil properties, and providing habitat for survival. These results confirm that BC-nZVI is effective in removing Cr(VI) and stabilizing Cr in soil with no significant adverse effects on soil quality or soil microorganisms.
Optimized Properties in Multifunctional Polyphenylene Sulfide Composites via Graphene Nanosheets/Boron Nitride Nanosheets Dual Segregated Structure under High Pressure
Authors: Zhang Liangqing , Yang Shugui , Peng Longgui , Zhong Kepeng , Chen Yanhui
Published: October 2022

The practical application of polymer composites in the electronic and communications industries often requires multi-properties, such as high thermal conductivity (TC), efficient electromagnetic interference (EMI) shielding ability with low electrical conductivity, superior tribological performance, reliable thermal stability and excellent mechanical properties. However, the integration of these mutually exclusive properties is still a challenge, ascribed to their different requirement on the incorporated nanofillers, composite microstructure as well as processing process. Herein, a well-designed boron nitride nanosheet (BN)/graphene nanosheet (GNP)/polyphenylene sulfide (PPS) composite with a dual-segregated structure is fabricated via high-pressure molding. Rather than homogenous mixing of the hybrid fillers, GNP is first coated on PPS particles and followed by encapsulating the conductive GNP layers with insulating BN, forming a BN shell-GNP layer-PPS core composite particles. After hot-pressing, a dual segregated structure is constructed, in which GNP and BN are distinctly separated and arranged in the interfaces of PPS, which on the one hand gives rise to high thermal conductivity, and on the other hand, the aggregated BN layer can act as an “isolation belt” to effectively reduce the electronic transmission. Impressively, high-pressure is loaded and it has a more profound effect on the EMI shielding and thermal conductive properties of PPS composites with a segregated structure than that with homogenous mixed-structure composites. Intriguingly, the synergetic enhancement effect of BN and GNP on both thermal conductive performance and EMI shielding is stimulated by high pressure. Consequently, PPS composites with 30 wt% GNP and 10 wt% BN hot-pressed under 600 MPa present the most superior comprehensive properties with a high TC of 6.4 W/m/K, outstanding EMI SE as high as 70 dB, marvelous tribological performance, reliable thermal stability and satisfactory mechanical properties, which make it promising for application in miniaturized electronic devices in complex environments.
Oxygen Vacancies in Bismuth Tantalum Oxide to Anchor Polysulfide and Accelerate the Sulfur Evolution Reaction in Lithium–Sulfur Batteries
Authors: Wang Chong , Lu Jian-Hao , Wang An-Bang , Zhang Hao , Wang Wei-Kun , Jin Zhao-Qing , Fan Li-Zhen
Published: October 2022

The shuttling effect of soluble lithium polysulfides (LiPSs) and the sluggish conversion kinetics of polysulfides into insoluble Li2S2/Li2S severely hinders the practical application of Li-S batteries. Advanced catalysts can capture and accelerate the liquid–solid conversion of polysulfides. Herein, we try to make use of bismuth tantalum oxide with oxygen vacancies as an electrocatalyst to catalyze the conversion of LiPSs by reducing the sulfur reduction reaction (SRR) nucleation energy barrier. Oxygen vacancies in Bi4TaO7 nanoparticles alter the electron band structure to improve instinct electronic conductivity and catalytic activity. In addition, the defective surface could provide unsaturated bonds around the vacancies to enhance the chemisorption capability with LiPSs. Hence, a multidimensional carbon (super P/CNT/Graphene) standing sulfur cathode is prepared by coating oxygen vacancies Bi4TaO7−x nanoparticles, in which the multidimensional carbon (MC) with micropores structure can host sulfur and provide a fast electron/ion pathway, while the outer-coated oxygen vacancies with Bi4TaO7−x with improved electronic conductivity and strong affinities for polysulfides can work as an adsorptive and conductive protective layer to achieve the physical restriction and chemical immobilization of lithium polysulfides as well as speed up their catalytic conversion. Benefiting from the synergistic effects of different components, the S/C@Bi3TaO7−x coin cell cathode shows superior cycling and rate performance. Even under a high level of sulfur loading of 9.6 mg cm−2, a relatively high initial areal capacity of 10.20 mAh cm−2 and a specific energy density of 300 Wh kg−1 are achieved with a low electrolyte/sulfur ratio of 3.3 µL mg−1. Combined with experimental results and theoretical calculations, the mechanism by which the Bi4TaO7 with oxygen vacancies promotes the kinetics of polysulfide conversion reactions has been revealed. The design of the multiple confined cathode structure provides physical and chemical adsorption, fast charge transfer, and catalytic conversion for polysulfides.
External Validation with Accuracy Confounders of VCO2-Derived Predicted Energy Expenditure Compared to Resting Energy Expenditure Measured by Indirect Calorimetry in Mechanically Ventilated Children
Authors: Briassoulis Panagiotis , Ilia Stavroula , Briassouli Efrossini , Briassoulis George
Published: October 2022

Optimal energy provision, guided by measured resting energy expenditure (REE) and determined by indirect calorimetry (IC), is fundamental in Intensive Care Units (ICU). Because IC availability is limited, methods to predict REE based on carbon dioxide production (VCO2) measurements (REEVCO2) alone have been proposed as a surrogate for REE measured by IC (REEIC). The study aimed at externally and internally validating the accuracy of the REEVCO2 as an alternative to REEIC in mechanically ventilated children. A ventilator’s integrated gas exchange module (E-COVX) was used to prospectively measure REEIC and predict REEVCO2 on 107 mechanically ventilated children during the first 24 h of admission. The accuracy of the REEVCO2 compared to REEIC was assessed through the calculation of bias and precision, paired median differences, linear regression, and ROC analysis. Accuracy within ±10% of the REEIC was deemed acceptable for the REEVCO2 equation. The calculated REEVCO2 based on respiratory quotient (RQ) 0.89 resulted in a mean bias of −72.7 kcal/day (95% limits of agreement −321.7 to 176.3 kcal/day) and a high coefficient of variation (174.7%), while 51.4% of the calculations fell outside the ±10% accuracy rate. REEVCO2 derived from RQ 0.80 or 0.85 did not improve accuracy. Only measured RQ (Beta 0.73, p < 0.001) and no-recorded neuromuscular blocking agents (Beta −0.13, p = 0.044) were independently associated with the REEVCO2−REEIC difference. Among the recorded anthropometric, metabolic, nutrition, or clinical variables, only measured RQ was a strong predictor of REEVCO2 inaccuracy (p < 0.001). Cutoffs of RQ = 0.80 predicted 89% of underestimated REEIC (sensitivity 0.99; specificity 0.89) and RQ = 0.82 predicted 56% of overestimated REEIC (sensitivity of 0.99; specificity 0.56). REEVCO2 cannot be recommended as an alternative to REEIC in mechanically ventilated children, regardless of the metabolic, anthropometric, or clinical status at the time of the evaluation.
Impact of a Very Low-Calorie Ketogenic Diet (VLCKD) on Changes in Handgrip Strength in Women with Obesity
Authors: Barrea Luigi , de Alteriis Giulia , Muscogiuri Giovanna , Vetrani Claudia , Verde Ludovica , Camajani Elisabetta , Aprano Sara , Colao Annamaria , Savastano Silvia
Published: October 2022

The preservation of muscle mass, which is positively associated with muscle strength, has been included among the benefits of ketogenic diets due to the synergistic effects exerted by the reduction in visceral adipose tissue and obesity-related pro-inflammatory status. The handgrip strength (HGS) test is widely used as a single indicator to represent overall muscle strength. The possible association of changes in HGS in patients with obesity during the consumption of a very low-calorie ketogenic diet (VLCKD) has not yet been investigated. The aim of this prospective study was to assess the efficacy of VLCKD on promoting changes in HGS and high-sensitivity C-reactive protein (hs-CRP) levels, as a serological marker of obesity-related, low-grade inflammation, in a population of women with obesity after 45 days of active phase of the VLCKD. This pilot, uncontrolled, single-center, open-label clinical trial examined 247 Caucasian women, aged 18–51 years (body mass index, BMI: 30.0–50.9 kg/m2) who were consecutively enrolled following 45 days of active phase the VLCKD. Anthropometric measures and physical activity were evaluated. Muscle strength was measured by HGS using a grip strength dynamometer. Body composition was evaluated using a bioelectrical impedance analysis (BIA) phase-sensitive system. hs-CRP levels were determined by nephelometric assay. Adherence to the VLCKD, ketosis status, and physical activity were checked weekly by phone call. At day 45, BMI, fat mass (FM), and hs-CRP levels were significantly decreased (∆-7.5 ± 3.1%, ∆-15.6 ± 9.0%, and ∆-39.9 ± 44.6%; respectively; p < 0.001 for all three parameters), while HGS had increased (∆+17.4 ± 13.2%; p < 0.001). After adjusting for ∆BMI, ∆waist circumference, ∆hs-CRP levels, and physical activity, the correlation among changes in ∆HGS (kg), ∆FM (kg), and ∆ fat free mass (FFM) (kg) remained statistically significant (r = −0.331, and r = 0.362, respectively; p < 0.001). Interestingly, the correlation between ∆HGS with ∆FM (r = −0.288, p < 0.001) and ∆FFM (r = 0.395, p < 0.001) were also independent of the percentage of weight loss. We are the first to report that, along with a significant reduction in body weight and an overall improvement in body composition and inflammatory status, the muscle strength evaluated by the HGS test increased in a population of women with obesity after 45 days of the active phase of the VLCKD, also following adjustment for common confounding variables.
Short-Term Effect of Additional Daily Dietary Fibre Intake on Appetite, Satiety, Gastrointestinal Comfort, Acceptability, and Feasibility
Authors: Borkoles Erika , Krastins Digby , van der Pols Jolieke C. , Sims Paul , Polman Remco
Published: October 2022

Background: There is evidence that high-fibre diets have significant health benefits, although the effect of increasing fibre on individuals’ appetite, satiety, and gastrointestinal comfort is not well established, nor is its acceptability and feasibility. Methods: This mixed-methods feasibility randomised control trial included 38 participants allocated to one of three conditions: FibreMAX (two daily servings of 25 g of BARLEYmax®), FibreGRAD (two daily servings with the amount of fibre gradually increased), and Control (two daily servings totalling 25 g of placebo product). Participants completed a food diary at baseline. The Hunger and Fullness Questionnaire and questions regarding gastrointestinal response were completed at baseline and at the end of each week. Participants completed the acceptability of intervention measure and engaged in a semi-structured interview, following trial completion. Results: The qualitative data suggested that increased fibre influenced appetite and fullness perceptions. Baseline fibre consumption and the method of increased fibre increase did not influence our findings. The qualitative results also indicated that the fibre intake was perceived as beneficial to well-being; it influenced feelings of hunger and caused some minor acute gastrointestinal symptoms that dissipated after a short adaption period. Conclusion: This study suggests that increasing fibre intake through BARLEYmax® is a safe intervention that is acceptable to participants.
Effectiveness of a Multi-Strategy Behavioral Intervention to Increase Vegetable Sales in Primary School Canteens: A Randomized Controlled Trial
Authors: Poelman Astrid A. M. , Djakovic Shadia , Heffernan Jessica E. , Cochet-Broch Maeva , Golley Rebecca K. , Cox David N. , Beelen Janne
Published: October 2022

Children’s vegetable intake remains inadequate and school canteens may provide opportunities to address this public health concern. This study aimed to determine the effectiveness of an 8-week multi-strategy behavioral intervention that included vegetable provisioning and online menu architecture on vegetable sales in primary school canteens. A randomized controlled trial was undertaken in 16 Australian primary schools (n = 4302 students). The control arm kept their regular canteen menu. The primary outcome was vegetable sales measured by assessing vegetable content (in grams) from all menu items and using canteen sales (ordered online and over-the-counter) to calculate vegetable sales (in grams/week) at baseline (3 weeks) and during intervention implementation (8 weeks). Secondary outcomes were vegetable sales in subcategories, intervention acceptability among canteen managers and vegetable waste (four schools). Linear mixed model analysis showed that from baseline to follow-up, the intervention group had significantly higher weekly vegetable sales overall compared with the control group (2707 g/week, 95% CI 1276 to 4137 g/week; p < 0.001), with increased vegetable sales in the subcategories of burgers, hot foods and snacks, but not in sandwiches and pasta/rice dishes. The intervention did not lead to more vegetable waste, nor to a decrease in canteen revenue. The canteen managers found the intervention easy to implement and felt children responded favorably to three of the seven strategies. In conclusion, a multi-strategy behavioral canteen intervention increased vegetable sales amongst primary school students.
Evidence Gaps in Clinical Trials of Pharmacologic Treatment for H1-Antihistamine-Refractory Chronic Spontaneous Urticaria: A Systematic Review and Future Perspectives
Authors: Nochaiwong Surapon , Chuamanochan Mati , Ruengorn Chidchanok , Thavorn Kednapa
Published: October 2022

No data addressing issues concerning disparities in participant and trial characteristics and trial outcome reporting have been established in clinical trials for H1-antihistamine-refractory chronic spontaneous urticaria (CSU). To better harmonize and compare the different treatment interventions, we systematically evaluated the overall landscape of pharmacological treatments for H1-antihistamine-refractory CSU clinical trials published between 2000 and 2021. This systematic review included 23 randomized clinical trials involving 2480 participants from 22 countries. We found significant increases in the number of globally published and newly tested drugs, especially biologic drugs. Regarding relatively small trials, we found that people living with H1-antihistamine-refractory CSU who were identified as members of minority groups (non-white population), populations of regions other than North America/Europe, and populations of low- to lower/upper-middle-income countries are underrepresented. Most trials were designed to evaluate treatment efficacy and safety profiles; however, less than half of the included trials reported the patient’s perspective in terms of patient-reported outcomes. Disparities in outcome reporting, including clinimetric tools for assessing treatment response and outcome sets, were observed. To close the evidence gap in H1-antihistamine-refractory CSU trials, strategies for improving trial and participant enrollment and standardizing core outcome sets for trial reporting are needed.
Controversial Interactions of Tacrolimus with Dietary Supplements, Herbs and Food
Authors: Miedziaszczyk Miłosz , Bajon Aleksander , Jakielska Ewelina , Primke Marta , Sikora Jędrzej , Skowrońska Dagmara , Idasiak-Piechocka Ilona
Published: October 2022

Tacrolimus is an immunosuppressive calcineurin inhibitor used to prevent rejection in allogeneic organ transplant recipients, such as kidney, liver, heart or lung. It is metabolized in the liver, involving the cytochrome P450 (CYP3A4) isoform CYP3A4, and is characterized by a narrow therapeutic window, dose-dependent toxicity and high inter-individual and intra-individual variability. In view of the abovementioned facts, the aim of the study is to present selected interactions between tacrolimus and the commonly used dietary supplements, herbs and food. The review was based on the available scientific literature found in the PubMed, Scopus and Cochrane databases. An increase in the serum concentration of tacrolimus can be caused by CYP3A4 inhibitors, such as grapefruit, pomelo, clementine, pomegranate, ginger and turmeric, revealing the side effects of this drug, particularly nephrotoxicity. In contrast, CYP3A4 inducers, such as St. John’s Wort, may result in a lack of therapeutic effect by reducing the drug concentration. Additionally, the use of Panax ginseng, green tea, Schisandra sphenanthera and melatonin in patients receiving tacrolimus is highly controversial. Therefore, since alternative medicine constitutes an attractive treatment option for patients, modern healthcare should emphasize the potential interactions between herbal medicines and synthetic drugs. In fact, each drug or herbal supplement should be reported by the patient to the physician (concordance) if it is taken in the course of immunosuppressive therapy, since it may affect the pharmacokinetic and pharmacodynamic parameters of other preparations.
Preparation of DSPE-PEG-cRGD Modified Cationic Liposomes for Delivery of OC-2 shRNA and The Antitumor Effects on Breast Cancer
Authors: Liu Chunyan , Zhao Wenli , Zhang Ligang , Sun Huamin , Chen Xi , Deng Ning
Published: October 2022

Cationic liposome delivery of interfering RNA (shRNA) plays an important role in tumor therapy. The cyclic Arg-Gly-Asp (cRGD) modified cationic liposomes (cRGD-CL) were designed for targeted delivery of ONECUT2 (OC-2) shRNA (pshOC-2) to breast cancer cells. The characterization analysis of cationic liposome showed that the prepared cRGD-CL/pshOC-2 lipoplexes had uniform particle size (150 ± 1.02 nm), moderate zeta potential (19.8 ± 0.249 mV) and high encapsulation efficiency (up to 96%). The results of flow cytometer showed that the introduction of cRGD could significantly promote the liposomes targeting tumor cells. In MCF-7 cells, the pshOC-2 could down-regulate expression of OC-2 and result in cell apoptosis, inhibition of the wound healing, migration and cell colony formation, in which the signal pathways of Bcl-xL, Bcl-2 were inhibited and the signal pathways of Bax and Cleaved Caspase-3 were promoted. In MCF-7 xenograft mice, intravenous administration of cRGD-CL/pshOC-2 lipoplexes could effectively reduce the expression of OC-2 in tumors and result in apparently antitumor effects, which suggested that the lipoplexes might be deeply penetrated into tumor through receptor-mediated transcytosis. The results revealed that the cationic liposome (cRGD-CL) was an effective delivery system for OC-2 shRNA, which might be an effective therapeutic candidate for breast cancer.
Influence of Vegetable Oils on In Vitro Performance of Lutein-Loaded Lipid Carriers for Skin Delivery: Nanostructured Lipid Carriers vs. Nanoemulsions
Authors: Teeranachaideekul Veerawat , Boribalnukul Putita , Morakul Boontida , Junyaprasert Varaporn Buraphacheep
Published: October 2022

Nanostructured lipid carriers (NLC) were prepared from solid lipid (glyceryl monostearate, GMS) and vegetable oils, including palm oil (PO), rice bran oil (RBO) or virgin coconut oil (VCO), at different ratios (95:5, 90:10 and 80:20), while nanoemulsions (NE) were prepared with sole vegetable oils. After production, the particle size of the lutein-free NLC and NE was found to be between 100 and 150 nm and increased after loading with lutein. An increase in oil loading in NLC reduced the particle size and resulted in a less ordered lipid matrix and an increase in % entrapment efficiency. From the stability study, it was observed that the types of oils and oil content in the lipid matrix had an impact on the chemical stability of lutein. Regarding the release study, lutein-loaded NE showed higher release than lutein-loaded NLC. Both NLC and NE prepared from VCO exhibited higher release than those prepared from PO and RBO, respectively (p < 0.05). In contrast, among the formulations of NLC and NE, both lutein-loaded NLC and NE prepared from RBO showed the highest permeation through the human epidermis due to the skin enhancement effect of RBO. Based on all the results, the lipid nanocarriers composed of RBO could effectively enhance the chemical stability of lutein and promote drug penetration into the skin.
Design of a Fiber Alkali Vapor Cell for Atomic Magnetometer for Magnetoencephalography Applications
Authors: Liu Xuejing , Hu Yanhui , Li Yang , Wu Xudong , Chang Min , Zhang Xuedian
Published: October 2022

Spin exchange relaxation free (SERF) atomic magnetometer (AM), based on the Larmor precession of alkali atoms, is considered a promising candidate for magnetoencephalography (MEG) systems with the advantages of high sensitivity and no need for cryogenic devices. The footprint of the sensor header contains alkali vapor cell and bulk optical elements determining the spatial resolution of the MEG system. Optical fiber could separate the vapor cell far from other parts of the sensor header to improve the spatial resolution. However, coupling between glass cell and fibers limits the coupling loss of the light. Here, we describe the design of a fiber-based alkali vapor cell that could alleviate these issues. A pair of fiber cables combining a polarization maintaining fiber (PMF) and hollow-core photonic crystal fibers (HC-PCFs) are enclosed in a vacuum-sealed T-shape glass tube filled with alkali atoms. The fiber cell ensures a flexible integration with most fiber systems. The fiber structure, with an air gap between HC-PCFs, provides a large interaction volume between light and atoms. The vapor of the alkali atoms diffuses into the air core of the HC-PCF from the glass tube by heating. The alkali atoms still contained in SERF regime are within the wall relaxation rates of 12,764 s−1 in the coating fiber cell. The insertion loss due to fiber coupling is analyzed. The coupling efficiency could be 91%, with the fiber structure consisting of a 40 μm diameter HC-PCF and a 1 mm air gap. The limit sensitivity under this condition is simulated at 14.7 fT/Hz1/2. The fabrication technique and the light insertion loss are discussed. The fiber alkali vapor cell is of compact size and has flexible integration with the fiber atomic spin precession detection system.
Improving Yield and Yield Stability in Winter Rye by Hybrid Breeding
Authors: Hackauf Bernd , Siekmann Dörthe , Fromme Franz Joachim
Published: October 2022

Rye is the only cross-pollinating small-grain cereal. The unique reproduction biology results in an exceptional complexity concerning genetic improvement of rye by breeding. Rye is a close relative of wheat and has a strong adaptation potential that refers to its mating system, making this overlooked cereal readily adjustable to a changing environment. Rye breeding addresses the emerging challenges of food security associated with climate change. The systematic identification, management, and use of its valuable natural diversity became a feasible option in outbreeding rye only following the establishment of hybrid breeding late in the 20th century. In this article, we review the most recent technological advances to improve yield and yield stability in winter rye. Based on recently released reference genome sequences, SMART breeding approaches are described to counterbalance undesired linkage drag effects of major restorer genes on grain yield. We present the development of gibberellin-sensitive semidwarf hybrids as a novel plant breeding innovation based on an approach that is different from current methods of increasing productivity in rye and wheat. Breeding of new rye cultivars with improved performance and resilience is indispensable for a renaissance of this healthy minor cereal as a homogeneous commodity with cultural relevance in Europe that allows for comparatively smooth but substantial complementation of wheat with rye-based diets, supporting the necessary restoration of the balance between human action and nature.
Cork Development: What Lies Within
Authors: Teixeira Rita Teresa
Published: October 2022

The cork layer present in all dicotyledonous plant species with radial growth is the result of the phellogen activity, a secondary meristem that produces phellem (cork) to the outside and phelloderm inwards. These three different tissues form the periderm, an efficient protective tissue working as a barrier against external factors such as environmental aggressions and pathogen attacks. The protective function offered by cork cells is mainly due to the abundance of suberin in their cell walls. Chemically, suberin is a complex aliphatic network of long chain fatty acids and alcohols with glycerol together with aromatic units. In most woody species growing in temperate climates, the first periderm is replaced by a new functional periderm upon a few years after being formed. One exception to this bark development can be found in cork oak (Quercus suber) which display a single periderm that grows continuously. Quercus suber stands by its thick cork layer development with continuous seasonal growth. Cork raw material has been exploited by man for centuries, especially in Portugal and Spain. Nowadays, its applications have widened vastly, from the most known product, stoppers, to purses or insulating materials used in so many industries, such as construction and car production. Research on how cork develops, and the effect environmental factors on cork oak trees is extremely important to maintain production of good-quality cork, and, by maintaining cork oak stands wealthy, we are preserving a very important ecosystem both by its biodiversity and its vital social and economic role in areas already showing a population declination.
Recursive Settling of Particles in Shear Thinning Polymer Solutions: Two Velocity Mathematical Model
Authors: Neverov Vladimir , Shelukhin Vladimir
Published: October 2022

Processing of the available experimental data on particles settling in shear-thinning polymer solutions is performed. Conclusions imply that sedimentation should be recursive, since settling also occurs within the sediment. To capture such an effect, a mathematical model of two continua has been developed, which corresponds to experimental data. The model is consistent with basic thermodynamics laws. The rheological component of this model is a correlation formula for gravitational mobility. This closure is justified by comparison with known experimental data available for particles settling in vertical vessels. In addition, the closure is validated by comparison with analytical solutions to the Kynch one-dimensional equation, which governs dynamics of particle concentration. An explanation is given for the Boycott effect and it is proven that sedimentation is enhanced in a 2D inclined vessel. In tilted vessels, the flow is essentially two-dimensional and the one-dimensional Kynch theory is not applicable; vortices play an important role in sedimentation.
The Aging Performance of PVDF in Acid Oil and Gas Medium
Authors: Qi Guoquan , Yan Hongxia , Qi Dongtao , Li Houbu , Zhang Zhao
Published: October 2022

In the process of transporting oil and gas, the service performance of thermoplastic pipes will decline due to the multiple influences of medium, temperature, and pressure. In order to study the service performance changes of PVDF pipes under oil and gas transportation conditions, the high-temperature autoclave is used to simulate the service state of the pipe in the mediums. The PVDF samples are exposed to simulated oil and gas mediums for 1 week, 3 weeks, 5 weeks, and 7 weeks under 60 °C and 90 °C conditions. After the exposure test, the physical and chemical properties of the PVDF pipe are tested and compared with the initial samples. Compared with the initial sample, the sample quality after the exposure test has a slight increase, with growth rates of 2% and 3% at 60 °C and 90 °C, respectively. Meanwhile, the tensile strength of the samples is about 13% and 21% lower than that of the initial sample, respectively. According to the microscopic morphology analyses, there are some crack defects on the surface of the sample after the exposure test. Through infrared analysis, it is shown that no molecular chain breakage, crosslinking, or other reactions are found after the exposure test. The above analysis shows that the PVDF sample has slight penetration and swelling during the exposure test. However, due to the large force between the PVDF molecules, its mechanical properties have a small downward trend, showing excellent environmental stress crack resistance.
Graphene-Based Membranes for Water Desalination: A Literature Review and Content Analysis
Authors: Dai Yexin , Liu Miao , Li Jingyu , Kang Ning , Ahmed Afaque , Zong Yanping , Tu Jianbo , Chen Yanzhen , Zhang Pingping , Liu Xianhua
Published: October 2022

Graphene-based membranes have unique nanochannels and can offer advantageous properties for the water desalination process. Although tremendous efforts have been devoted to heightening membrane performance and broadening their application, there is still lack of a systematic literature review on the development and future directions of graphene-based membranes for desalination. In this mini-review, literature published between 2011 and 2022 were analyzed by using the bibliometric method. We found that the major contributors to these publications and the highest citations were from China and the USA. Nearly 80% of author keywords in this analysis were used less than twice, showing the broad interest and great dispersion in this field. The recent advances, remaining gaps, and strategies for future research, were discussed. The development of new multifunctional nanocomposite materials, heat-driven/solar-driven seawater desalination, and large-scale industrial applications, will be important research directions in the future. This literature analysis summarized the recent development of the graphene-based membranes for desalination application, and will be useful for researchers in gaining new insights into this field.
Electro Fluid Dynamics: A Route to Design Polymers and Composites for Biomedical and Bio-Sustainable Applications
Authors: Renkler Nergis Zeynep , Cruz-Maya Iriczalli , Bonadies Irene , Guarino Vincenzo
Published: October 2022

In the last two decades, several processes have been explored for the development of micro and/or nanostructured substrates by sagely physically and/or chemically manipulating polymer materials. These processes have to be designed to overcome some of the limitations of the traditional ones in terms of feasibility, reproducibility, and sustainability. Herein, the primary aim of this work is to focus on the enormous potential of using a high voltage electric field to manipulate polymers from synthetic and/or natural sources for the fabrication of different devices based on elementary units, i.e., fibers or particles, with different characteristic sizes—from micro to nanoscale. Firstly, basic principles and working mechanisms will be introduced in order to correlate the effect of selected process parameters (i.e., an applied voltage) on the dimensional features of the structures. Secondly, a comprehensive overview of the recent trends and potential uses of these processes will be proposed for different biomedical and bio-sustainable application areas.
Hydroxypropyl-β-Cyclodextrin for Delivery of Sinapic Acid via Inclusion Complex Prepared by Solvent Evaporation Method
Authors: Ahad Abdul , Bin Jardan Yousef A. , Raish Mohammad , Al-Mohizea Abdullah M. , Al-Jenoobi Fahad I.
Published: October 2022

The goal of this study was to increase the aqueous solubility and dissolution rate of sinapic acid (SA) by formulating binary inclusion complex (BIC) of SA with hydroxypropyl-β-cyclodextrin (HPβCD) using solvent evaporation (SE) technology. The phase solubility and dissolution studies were conducted to determine the solubility and in vitro release rate of SA. In addition, the prepared inclusion complex was characterized for solid state characterization using techniques such as DSC, PXRD, SEM, and FTIR. Moreover, the prepared SA-BIC was evaluated for its antioxidant activity. Results revealed that the SA solubility can be shown to improve with a change in HPβCD concentration. About 2.59 times higher solubility of SA in water was noticed in the presence of HPβCD (10 mM). Dissolution study demonstrated that the 34.11 ± 4.51% of SA was released from binary physical mixture (BPM), while the maximum release of 46.27 ± 2.79% of SA was observed for SA-BIC prepared by SE method. The prepared SA-BIC demonstrated distinctive properties when compared to pure SA, which was demonstrated by different analytical methods, such as DSC, PXRD, SEM, and FTIR, as evidence of SA inclusion into HPβCD cavity. Further, it was observed that SA-BIC displayed stronger DPPH radical scavenging activity than SA. In conclusion, SE technology considerably enhanced the complexity of SA with HPβCD, and these observations could help to heighten the SA solubility, which may lead to a better bioavailability.
“Were Buddhist Scriptures in China Translated for Chinese?”: A New Investigation into the Development of Early Buddhist Chinese from the Perspective of the Buddhist Speech Community
Authors: Jiang Zhejie
Published: October 2022

Modern scholarship focuses on the lexical or syntactical features of Buddhist Chinese used in Chinese translations of Buddhist scriptures; however, the origins of the language have attracted relatively little attention. Our article explores the issue from the perspective of the speech community, and we argue that the community’s ethnicity played an important role in the pre-fourth-century development of Buddhist Chinese. Buddhist scriptures were mainly introduced to inland China via the Western Regions and the Han people were not officially allowed to be monks. In addition to the translated scripture’s readership, considerable numbers of scripture transmitters and translators were not Han Chinese, and Han Chinese translation assistants were mainly grassroots literati. We combined an analysis of language learning with an examination of the universal features of translated languages and views on translation, and we argue that the early Buddhist community generally could not write and did not need to read refined literary Chinese. The lifting of a ban on Han peoples converting to Buddhism from the fourth century onwards accelerated the development of Han Buddhist communities, and dramatically promoted the localization of Buddhism and scriptures, which marked a new era of translation and translated language. Our investigation into the framework of the Buddhist Chinese speech community provides new perspectives compared with philological studies on the Buddhist language and explains the early historical development of Buddhist Chinese.
Jonas, Scholem, and the Taubeses in Jerusalem: From Metaphysical Antisemitism to a Jewish Gnostic Conspiracy
Authors: Cahana-Blum Jonathan
Published: October 2022

This article addresses how Hans Jonas’s reconstruction of gnosticism as a historical movement in late antiquity gave rise to two parallel contemporary interpretations: Gershom Scholem’s “metaphysical antisemitism” and Susan and Jacob Taubes’s attempt at a revival of a Jewish—gnostic cultic revolt. While both reached very similar conclusions regarding the gnostic potential for modern Judaism, they could not be more different in the implications they drew from it. This, in turn, also explains the animosity that developed between all the people involved.
Co-Generating Knowledge in Nexus Research for Sustainable Wastewater Treatment
Authors: Avellán Tamara , Hahn Angela , Kirschke Sabrina , Müller Andrea , Benavides Lucia , Caucci Serena
Published: October 2022

Currently installed wastewater treatment systems in many developing countries are unsustainable, failing in either the social, economic or ecological dimension of sustainability. Nexus research looking at resources involved in wastewater treatment could support the transition towards more sustainable systems. Nexus thinking aims to overcome bio-physical systems thinking by including transdisciplinary research methods. Approaches for integrating results from different types of analysis and disciplinary backgrounds are scarce and have not been described extensively in nexus research. Transdisciplinary research suggests creating system, target and transformation knowledge as a common framework to describe meaningful transformations. Our goal is to show how a better understanding of the level of knowledge created by different types of analysis can pave the way towards integrating results for sustainability. In this article, three types of analysis, namely sustainability assessment, stakeholder perspective analysis and wickedness analysis, were applied in two pilot case wastewater treatment systems in Latin America. Through a three-step process, generated knowledge was assessed for each type of analysis individually while also highlighting synergies between them. The results demonstrate that structuring results by generated knowledge type can help combining outcomes in a meaningful manner. The findings show that technical flaws are present and fixable, and that issues relating to behaviours or values are more challenging to address but arguably more meaningful for systemic change.