bluuebunny/crossref_metadata_2025 huggingface dataset:
Development and validation of a clinical nomogram for differentiating hemorrhagic and ischemic stroke prehospital
Authors: Ye Sheng , Pan Huiqing , Li Weijia , Wang Jinqiang , Zhang Hailong
Published: March 2023

Objectives

The early detection and identification of stroke are essential to the prognosis of patients with suspected stroke symptoms out-of-hospital. We aimed to develop a risk prediction model based on the FAST score to identify the different types of strokes early for emergency medical services (EMS).

Methods

This retrospective observational study enrolled 394 stroke patients at a single center from January 2020 to December 2021. Demographic data, clinical characteristics, and stroke risk factors with patients were collected from the EMS record database. Univariate and multivariate logistic regression analysis was used to identify the independent risk predictors. The nomogram was developed based on the independent predictors, in which the discriminative value and calibration of the nomogram were verified by the receiver operator characteristic (ROC) curve and calibration plots.

Results

A total of 31.90% (88/276) of patients were diagnosed with hemorrhagic stroke in the training set, while 36.40% (43/118) in the validation set. The nomogram was developed based on the multivariate analysis, including age, systolic blood pressure, hypertension, vomiting, arm weakness, and slurred speech. The area under the curve (AUC) of the ROC with nomogram was 0.796 (95% CI: 0.740–0.852, P < 0.001) and 0.808 (95% CI:0.728–0.887, P < 0.001) in the training set and validation set, respectively. In addition, the AUC with the nomogram was superior to the FAST score in both two sets. The calibration curve showed a good agreement with the nomogram and the decision curves analysis also demonstrated that the nomogram had a wider range of threshold probabilities than the FAST score in the prediction risk of hemorrhagic stroke.

Conclusions

This novel noninvasive clinical nomogram shows a good performance in differentiating hemorrhagic and ischemic stroke for EMS staff prehospital. Moreover, all of the variables of nomogram are acquired in clinical practice easily and inexpensively out-of-hospital.
Clinical characteristics and therapeutic effects of checkpoint inhibitor-related pneumonitis in patients with non-small cell lung cancer
Authors: Pang Li , Xie Mei , Ma Xidong , Huang Aiben , Song Jialin , Yao Jie , Deng Hui , Zhang Duchao , Zang Xuelei , Ren Fangping , Gao Jie , Wu Chongchong , Wang Yuanyong , Zhang Xin , Bao Xinyu , Pan Lei , Xue Xinying
Published: March 2023

Background

With the application of immune checkpoint inhibitors (ICIs) in cancer treatment, more and more attention has been paid to checkpoint inhibitor-related pneumonitis (CIP), which requires a better understanding of its clinical characteristics and therapeutic effects.

Methods

The clinical and imaging data of 704 patients with non-small cell lung cancer (NSCLC) who received immunotherapy were analyzed retrospectively; the clinical characteristics of CIP were summarized, and the therapeutic regimens and effects of the patients were summarized.

Results

36 CIP patients were included in the research. The most common clinical symptoms were cough, shortness of breath and fever. The CT manifestations were summarized as follows: Organizing pneumonia (OP) in 14 cases (38.9%), nonspecific interstitial pneumonia (NSIP) in 14 cases (38.9%), hypersensitiviy pneumonitis(HP) in 2 cases (6.3%), diffuse alveolar damage in 1 case (3.1%) and atypical imaging manifestations in 5 cases (13.9%). 35 cases received glucocorticoid therapy, 6 patients were treated with gamma globulin and 1 patient was treated with tocilizumab. There were no deaths in CIP G1-2 patients and 7 deaths occured in CIP G3-4 patients. 4 patients were treated again with ICIs.

Conclusion

We found that glucocorticoid 1–2 mg/kg was effective for most patients with moderate to severe CIP, and a few patients with hormone insensitivity needed early immunosuppressive therapy. A few patients can be rechallenged with ICIs, but CIP recurrence needs to be closely monitored.
A text-mining study on emotional cognition, understanding, and preventative behaviors during the COVID-19 pandemic
Authors: Lim Eunjung , Shin Jieun , Park Seyeon
Published: March 2023

Background

This study aimed to look at emotions perceived about the attributes, prevention, diagnosis, and treatment of infectious diseases related to coronavirus disease (COVID-19) that were widespread across the world and identify their relevance to knowledge about infectious diseases and preventative behaviors.

Methods

Texts to measure emotional cognition were selected through a pre-test, and 282 people were chosen as participants based on the survey conducted for 20 days from August 19 to August 29, 2020, created with Google Forms. IBM SPSS Statistics 25.0 was used for the primary analysis, and the SNA package in R (version 4.0.2) was utilized to conduct the network analysis.

Results

It was found that universal negative emotions such as feeling “anxious” (65.5%), “afraid” (46.1%), and “scared” (32.7%) commonly appeared among most people. Also, they were found to be feeling both positive (“caring” [42.3%] and “strict” [28.2%]) and negative (“frustrating” [39.1%] and “isolated” [31.0%]) emotions about efforts to prevent and curb the spread of COVID-19. In terms of emotional cognition for the diagnosis and treatment of such diseases, “reliable” (43.3%) took the biggest ratio among the replies. The level of understanding about infectious diseases showed differences in emotional cognition, thereby affecting people’s emotions. However, no differences were found in the practice of preventative behaviors.

Conclusions

Emotions associated with cognition in the context of pandemic infectious diseases have been found to be mixed. Furthermore, it can be seen that feelings vary depending on the degree of understanding of the infectious disease.
The experience of diabetic retinopathy patients during hospital-to-home full-cycle care: A qualitative study
Authors: Zhang Mengyue , Zhang ChunHua , Chen Chen , Liu Linjie , Liang Youping , Hong YiRong , Chen Yanyan , Shi Yinghui
Published: March 2023

Background

Diabetic retinopathy (DR) is one of the major blinding eye diseases worldwide. Psychological, emotional and social problems of DR patients are prominent. The aim of this study is to explore the experiences of patients with different phases of DR from hospital to home based on the “Timing It Right” framework, and to provide a reference for formulating corresponding intervention strategies.

Methods

The phenomenological method and semi-structured interviews were used in this study. A total of 40 patients with DR in different phases were recruited from a tertiary eye hospital between April and August 2022. Colaizzi’s analysis method was used to analyse the interview data.

Results

Based on the “Timing It Right” framework, different experiences in five phases of DR before and after Pars Plana Vitrectomy (PPV) were extracted. The patients experienced complicated emotional reactions and inadequate coping skills during the pre-surgery phase, increased uncertainty during the post-surgery phase, insufficient confidence and the decision to change during the discharge preparation phase, eagerness for professional support and moving forward in exploration during the discharge adjustment phase, and courageous acceptance and positive integration during the discharge adaptation phase.

Conclusion

The experiences of DR patients with vitrectomy in different phases of disease are ever-changing, and medical staff should provide personalized support and guidance to help DR patients get through the hard times smoothly and enhance the quality of hospital-family holistic care.
Purification and characterization of aspartic protease from Aspergillus niger and its efficient hydrolysis applications in soy protein degradation
Authors: Wei Mengyuan , Chen Pengcheng , Zheng Pu , Tao Xiumei , Yu Xiaowei , Wu Dan
Published: March 2023

Background

Adding acid protease to feed can enhance protein digestibility, boost feed utilization, and stimulate the growth of animals in breading industry. In order to obtain an acid protease with high hydrolysis efficiency to plant protein, in this study, an aspartic protease from Aspergillus niger was heterologous expressed in Pichia pastoris (P. pastoris). The enzymatic properties and application in soybean protein degradation were also studied.

Results

In our investigation, the high aspartic protease (Apa1) activity level of 1500 U/mL was achieved in 3 L bioreactor. After dialysis and anion exchange chromatography, the total enzyme activity and specific enzyme activity were 9412 U and 4852 U/mg, respectively. The molecular weight of the purified protease was 50 kDa, while the optimal pH and temperature were 3.0 and 50 °C, respectively. It was stable at pH 2.0–5.0 and 30–60 °C. Apa1 was used to hydrolyze soybean isolate protein (SPI) at 40 °C and pH 3.0, and a high hydrolysis degree (DH) of 61.65% was achieved. In addition, the molecular weight distribution of SPI hydrolysis products was studied, the result showed that the hydrolysis products were primarily oligopeptides with molecular weights of 189 Da or below.

Conclusions

In this study, Apa1 was successfully expressed in P. pastoris and high expression level was obtained. In addition, the highest protein hydrolysis rate to SPI degradation so far was achieved. The acid protease in this study provides a new protease that is suitable for the feed industry, which will be very helpful to improve the feed utilization and promote the development of the breeding industry.
The effects of first-line pharmacological treatments for reproductive outcomes in infertile women with PCOS: a systematic review and network meta-analysis
Authors: Peng Ge , Yan Zhe , Liu Yuqi , Li Juan , Ma Jinfang , Tong Nanwei , Wang Yan
Published: March 2023

Background

Polycystic ovarian syndrome (PCOS) is one of the most common causes of infertility in reproductive-age women. However, the efficacy and optimal therapeutic strategy for reproductive outcomes are still under debate. We conducted a systematic review and network meta-analysis to compare the efficacy of different first-line pharmacological therapies in terms of reproductive outcomes for women with PCOS and infertility.

Methods

A systematic retrieval of databases was conducted, and randomized clinical trials (RCTs) of pharmacological interventions for infertile PCOS women were included. The primary outcomes were clinical pregnancy and live birth, and the secondary outcomes were miscarriage, ectopic pregnancy and multiple pregnancy. A network meta-analysis based on a Bayesian model was performed to compare the effects of the pharmacological strategies.

Results

A total of 27 RCTs with 12 interventions were included, and all therapies tended to increase clinical pregnancy, especially pioglitazone (PIO) (log OR 3.14, 95% CI 1.56 ~ 4.70, moderate confidence), clomiphene citrate (CC) + exenatide (EXE) (2.96, 1.07 ~ 4.82, moderate confidence) and CC + metformin (MET) + PIO (2.82, 0.99 ~ 4.60, moderate confidence). Moreover, CC + MET + PIO (2.8, -0.25 ~ 6.06, very low confidence) could increase live birth most when compared to placebo, even without a significant difference. For secondary outcomes, PIO showed a tendency to increase miscarriage (1.44, -1.69 ~ 5.28, very low confidence). MET (-11.25, -33.7 ~ 0.57, low confidence) and LZ + MET (-10.44, -59.56 ~ 42.11, very low confidence) were beneficial for decreasing ectopic pregnancy. MET (0.07, -4.26 ~ 4.34, low confidence) showed a neutral effect in multiple pregnancy. Subgroup analysis demonstrated no significant difference between these medications and placebo in obese participants.

Conclusions

Most first-line pharmacological treatments were effective in improving clinical pregnancy. CC + MET + PIO should be recommended as the optimal therapeutic strategy to improve pregnancy outcomes. However, none of the above treatments had a beneficial effect on clinical pregnancy in obese PCOS.

Trial registration

CRD42020183541; 05 July 2020
Induction of DNMT1-dependent demethylation of SHP-1 by the natural flavonoid compound Baicalein overcame Imatinib-resistance in CML CD34+ cells
Authors: Xu Xuefen , Ji Shufan , Chen Yuan , Xia Siwei , Li Yang , Chen Li , Li Yujia , Zhang Feng , Zhang Zili , Zheng Shizhong
Published: March 2023

Background

The most significant cause of treatment failure in chronic myeloid leukemia (CML) is a persistent population of minimal residual cells. Emerging evidences showed that methylation of SHP-1 contributed to Imatinib (IM) resistance. Baicalein was reported to have an effect on reversal of chemotherapeutic agents resistance. However, the molecular mechanism of Baicalein on JAK2/STAT5 signaling inhibition against drug resistance in bone marrow (BM) microenvironment that had not been clearly revealed.

Methods

We co-cultured hBMSCs and CML CD34+ cells as a model of SFM-DR. Further researches were performed to clarify the reverse mechanisms of Baicalein on SFM-DR model and engraftment model. The apoptosis, cytotoxicity, proliferation, GM-CSF secretion, JAK2/STAT5 activity, the expression of SHP-1 and DNMT1 were analyzed. To validate the role of SHP-1 on the reversal effect of Baicalein, the SHP-1 gene was over-expressed by pCMV6-entry shp-1 and silenced by SHP-1 shRNA, respectively. Meanwhile, the DNMT1 inhibitor decitabine was used. The methylation extent of SHP-1 was evaluated using MSP and BSP. The molecular docking was replenished to further explore the binding possibility of Baicalein and DNMT1.

Results

BCR/ABL-independent activation of JAK2/STAT5 signaling was involved in IM resistance in CML CD34+ subpopulation. Baicalein significantly reversed BM microenvironment-induced IM resistance not through reducing GM-CSF secretion, but interfering DNMT1 expression and activity. Baicalein induced DNMT1-mediated demethylation of the SHP-1 promoter region, and subsequently activated SHP-1 re-expression, which resulted in an inhibition of JAK2/STAT5 signaling in resistant CML CD34+ cells. Molecular docking model indicated that DNMT1 and Baicalein had binding pockets in 3D structures, which further supported Baicalein might be a small-molecule inhibitor targeting DNMT1.

Conclusions

The mechanism of Baicalein on improving the sensitivity of CD34+ cells to IM might be correlated with SHP-1 demethylation by inhibition of DNMT1 expression. These findings suggested that Baicalein could be a promising candidate by targeting DNMT1 to eradicate minimal residual disease in CML patients.
CD206+ tumor-associated macrophages interact with CD4+ tumor-infiltrating lymphocytes and predict adverse patient outcome in human laryngeal squamous cell carcinoma
Authors: Heng Yu , Zhu Xiaoke , Lin Hanqing , jingyu Ma , Ding Xuping , Tao Lei , Lu Liming
Published: March 2023

Background

Tumor-associated macrophages (TAMs) are major component in the tumor microenvironment (TME) and play regulatory role in tumor progression. We aimed to investigate the infiltration and prognostic value of TAMs in laryngeal squamous cell carcinoma (LSCC) and to reveal the underlying mechanism of TAM subgroups in tumorigenesis.

Methods

Hematoxylin and eosin (HE) staining were performed to define the tumor nest and stroma of LSCC tissue microarrays. CD206 + /CD163 + and iNOS + TAM infiltrating profiles were obtained and analyzed through double-labeling immunofluorescence and immunohistochemical staining. The recurrence-free (RFS) and overall survival (OS) curves based on the infiltration of TAMs were plotted using the Kaplan-Meier method. Infiltration of macrophages, T lymphocytes and their corresponding subgroups were analyzed in fresh LSCC tissue samples by flow cytometry.

Results

We found that CD206+ rather than CD163+ M2-like TAMs were the most enriched population in the TME of human LSCC. CD206+ macrophages localized mostly in the tumor stroma (TS) rather than the tumor nest (TN) region. In contrast, relatively low infiltration of iNOS+ M1-like TAMs were found in the TS and almost none in the TN region. High level of TS CD206+ TAM infiltration correlated with poor prognosis. Interestingly, we identified a HLA-DRhigh CD206+ macrophage subgroup that was significantly associated with the tumor-infiltrating CD4+ T lymphocytes and showed different surface costimulatory molecule expression than that of the HLA-DRlow/-CD206+ subgroup. Taken together, our results indicate that HLA-DRhigh-CD206+ is a highly activated subgroup of CD206 + TAMs that may interact with CD4 + T cells through MHC-II axis and promote tumorigenesis.
Speed and quality of interbody fusion in porous bioceramic Al2O3 and polyetheretherketone cages for anterior cervical discectomy and fusion: a comparative study
Authors: Kostysyn Roman , Ryska Pavel , Jandura Jiri , Selke-Krulichova Iva , Poczos Pavel , Hosszu Tomas , Cesak Tomas
Published: March 2023

Background

The objective of this prospective randomized monocentric study is to compare the speed and quality of interbody fusion of implanted porous Al2O3 (aluminium oxide) cages with PEEK (polyetheretherketone) cages in ACDF (anterior cervical discectomy and fusion).

Materials and methods

A total of 111 patients were enrolled in the study, which was carried out between 2015 and 2021. The 18-month follow-up (FU) was completed in 68 patients with an Al2O3 cage and 35 patients with a PEEK cage in one-level ACDF. Initially, the first evidence (initialization) of fusion was evaluated on computed tomography. Subsequently, interbody fusion was evaluated according to the fusion quality scale, fusion rate and incidence of subsidence.

Results

Signs of incipient fusion at 3 months were detected in 22% of cases with the Al2O3 cage and 37.1% with the PEEK cage. At 12-month FU, the fusion rate was 88.2% for Al2O3 and 97.1% for PEEK cages, and at the final FU at 18 months, 92.6% and 100%, respectively. The incidence of subsidence was observed to be 11.8% and 22.9% of cases with Al2O3 and PEEK cages, respectively.

Conclusions

Porous Al2O3 cages demonstrated a lower speed and quality of fusion in comparison with PEEK cages. However, the fusion rate of Al2O3 cages was within the range of published results for various cages. The incidence of subsidence of Al2O3 cages was lower compared to published results. We consider the porous Al2O3 cage as safe for a stand-alone disc replacement in ACDF.
How high is the quality of the videos about children's elbow fractures on Youtube?
Authors: Kıvrak Aybars , Ulusoy İbrahim
Published: March 2023

Objective

Pediatric elbow fractures are children's most common fractures. People use the internet to get information about their illnesses and also to search for treatment options. Videos uploaded to Youtube do not go through the review process. The aim of our study is to determine the quality of videos related to child elbow fractures on Youtube.

Material method

The study was carried out with the data obtained from the video-sharing site www.youtube.com on 01.12.2022. Pediatric elbow fractures are written in the search engine section. Information about the number of views of the videos, upload date, view rate (number of views per day), number of comments, number of likes and dislikes, duration, whether they contain animation and the publishing source were evaluated. The videos are divided into 5 groups according to their sources: medical society/non-profit organization, physician, healthrelated website, university/academic, and patient/independent user/other. The quality of the videos was evaluated using the Global Quality Scale (GQS). All videos have been evaluated by 2 researchers.

Results

In the study, 50 videos were included. In the statistical evaluation, no significant correlation was found between the modified discern score and GQS found by both researchers, and the number of views, the rate of views, comments, likes and dislikes, video duration and VPI. In addition, when the GQS and modified discern scores were compared according to the source of the video, it was found that the patient/independent user/other group scores were lower numerically, but there was no statistically significant difference in comparison.

Conclusion

Most of the videos about child elbow fractures have been uploaded by healthcare professionals. Therefore, we concluded that the videos are quite informative in terms of accurate information and quality content.
Novel agents and regimens in acute myeloid leukemia: latest updates from 2022 ASH Annual Meeting
Authors: DiNardo Katherine W. , LeBlanc Thomas W. , Chen Hui
Published: March 2023

Developments in investigational agents and novel regimens in acute myeloid leukemia (AML) were reported in the 2022 American Society of Hematology (ASH) annual meeting. Encouraging efficacy data were presented from first-in-human studies of two investigational menin inhibitors, SNDX-5613 and KO-539, in relapsed and refractory (R/R) acute myeloid leukemia (AML) with KMT2A rearrangement or mutant NPM1, with overall response rates (ORR) of 53% (32/60) and 40% (8/20), respectively. The addition of the novel drug pivekimab sunirine, a first-in-class antibody–drug conjugate targeting CD123, to azacitidine and venetoclax in R/R AML resulted in an ORR of 45% (41/91), which rose to 53% in those who were venetoclax naïve. Additional novel triplet treatment combinations included the addition of magrolimab, an anti-CD47 antibody, to azacitidine and venetoclax, with an ORR of 81% (35/43) in newly diagnosed AML, including an ORR of 74% (20/27) in TP53 mutated AML. The addition of the FLT3 inhibitor gilteritinib to azacitidine/venetoclax was also featured, with an ORR of 100% (27/27) in newly diagnosed AML and an ORR of 70% (14/20) in R/R AML.
NAD pool as an antitumor target against cancer stem cells in head and neck cancer
Authors: Navas Lola E. , Blanco-Alcaina Elena , Suarez-Martinez Elisa , Verdugo-Sivianes Eva M. , Espinosa-Sanchez Asuncion , Sanchez-Diaz Laura , Dominguez-Medina Eduardo , Fernandez-Rozadilla Ceres , Carracedo Angel , Wu Lindsay E. , Carnero Amancio
Published: March 2023

Head and neck squamous cell carcinoma (HNSCC) is a heterogeneous group of tumors that affect different anatomical locations. Despite this heterogeneity, HNSCC treatment depends on the anatomical location, TNM stage and resectability of the tumor. Classical chemotherapy is based on platinum-derived drugs (cisplatin, carboplatin and oxaliplatin), taxanes (docetaxel, paclitaxel) and 5-fluorouracil1. Despite advances in HNSCC treatment, the rate of tumor recurrence and patient mortality remain high. Therefore, the search for new prognostic identifiers and treatments targeting therapy-resistant tumor cells is vital. Our work demonstrates that there are different subgroups with high phenotypic plasticity within the CSC population in HNSCC. CD10, CD184, and CD166 may identify some of these CSC subpopulations with NAMPT as a common metabolic gene for the resilient cells of these subpopulations. We observed that NAMPT reduction causes a decrease in tumorigenic and stemness properties, migration capacity and CSC phenotype through NAD pool depletion. However, NAMPT-inhibited cells can acquire resistance by activating the NAPRT enzyme of the Preiss-Handler pathway. We observed that coadministration of the NAMPT inhibitor with the NAPRT inhibitor cooperated inhibiting tumor growth. The use of an NAPRT inhibitor as an adjuvant improved NAMPT inhibitor efficacy and reduced the dose and toxicity of these inhibitors. Therefore, it seems that the reduction in the NAD pool could have efficacy in tumor therapy. This was confirmed by in vitro assays supplying the cells with products of inhibited enzymes (NA, NMN or NAD) and restoring their tumorigenic and stemness properties. In conclusion, the coinhibition of NAMPT and NAPRT improved the efficacy of antitumor treatment, indicating that the reduction in the NAD pool is important to prevent tumor growth.
Identification of specific susceptibility loci for the early-onset colorectal cancer
Authors: Wang Haoxue , Cai Yimin , Jin Meng , Huang Chao Qun , Ning Caibo , Niu Siyuan , Fan Linyun , Li Bin , Zhang Ming , Lu Zequn , Dong Xuesi , Luo Zilin , Zhong Rong , Li Heng , Zhu Ying , Miao Xiaoping , Yang Xiaojun , Chang Jiang , Li Ni , Tian Jianbo
Published: March 2023

Background

The incidence of early-onset colorectal cancer (EOCRC; patients < 50 years old) has been rising rapidly, whereas the EOCRC genetic susceptibility remains incompletely investigated. Here, we aimed to systematically identify specific susceptible genetic variants for EOCRC.

Methods

Two parallel GWASs were conducted in 17,789 CRC cases (including 1490 EOCRC cases) and 19,951 healthy controls. A polygenic risk score (PRS) model was built based on identified EOCRC-specific susceptibility variants by using the UK Biobank cohort. We also interpreted the potential biological mechanisms of the prioritized risk variant.

Results

We identified 49 independent susceptibility loci that were significantly associated with the susceptibility to EOCRC and the diagnosed age of CRC (both P < 5.0×10−4), replicating 3 previous CRC GWAS loci. There are 88 assigned susceptibility genes involved in chromatin assembly and DNA replication pathways, mainly associating with precancerous polyps. Additionally, we assessed the genetic effect of the identified variants by developing a PRS model. Compared to the individuals in the low genetic risk group, the individuals in the high genetic risk group have increased EOCRC risk, and these results were replicated in the UKB cohort with a 1.63-fold risk (95% CI: 1.32–2.02, P = 7.67×10−6). The addition of the identified EOCRC risk loci significantly increased the prediction accuracy of the PRS model, compared to the PRS model derived from the previous GWAS-identified loci. Mechanistically, we also elucidated that rs12794623 may contribute to the early stage of CRC carcinogenesis via allele-specific regulating the expression of POLA2.

Conclusions

These findings will broaden the understanding of the etiology of EOCRC and may facilitate the early screening and individualized prevention.

Graphical Abstract
DNA methylation landscape reveals LIN7A as a decitabine-responsive marker in patients with t(8;21) acute myeloid leukemia
Authors: He Shujiao , Li Yan , Shi Xuanren , Wang Lei , Cai Diya , Zhou Jingfeng , Yu Li
Published: March 2023

Background

Despite its inconsistent response rate, decitabine, a demethylating agent, is often used as a non-intensive alternative therapeutic agent for acute myeloid leukemia (AML). It has been reported that relapsed/refractory AML patients with t(8;21) translocation achieved better clinical outcomes with a decitabine-based combination regimen than other AML subtypes; however, the mechanisms underlying this phenomenon remain unknown. Herein, the DNA methylation landscape of de novo patients with the t(8;21) translocation was compared with that of patients without the translocation. Moreover, the methylation changes induced by decitabine-based combination regimens in de novo/complete remission paired samples were investigated to elucidate the mechanisms underlying the better responses observed in t(8;21) AML patients treated with decitabine.

Methods

Thirty-three bone marrow samples from 28 non-M3 AML patients were subjected to DNA methylation sequencing to identify the differentially methylated regions and genes of interest. TCGA-AML Genome Atlas-AML transcriptome dataset was used to identify decitabine-sensitive genes that were downregulated following exposure to a decitabine-based regimen. In addition, the effect of decitabine-sensitive gene on cell apoptosis was examined in vitro using Kasumi-1 and SKNO-1 cells.

Results

A total of 1377 differentially methylated regions that specifically responsive to decitabine in t(8;21) AML were identified, of which 210 showed hypomethylation patterns following decitabine treatment aligned with the promoter regions of 72 genes. And the methylation-silencing genes, LIN7A, CEBPA, BASP1, and EMB were identified as critical decitabine-sensitive genes in t(8;21) AML. Moreover, AML patients with hypermethylated LIN7A and reduced LIN7A expression had poor clinical outcomes. Meanwhile, the downregulation of LIN7A inhibited decitabine/cytarabine combination treatment-induced apoptosis in t(8;21) AML cells in vitro.

Conclusion

The findings of this study suggest that LIN7A is a decitabine-sensitive gene in t(8;21) AML patients that may serve as a prognostic biomarker for decitabine-based therapy.
Multiple maxillary periodontal abscesses as a manifestation of post-coronavirus disease 2019 mucormycosis: a case report
Authors: Khoshkhou Hossein , Hasheminasab Mahboube , Goudarzi Pour Daryoush , Jamali Raika , Morshedzadeh Tehrani Ghazal , Moslemi Neda
Published: March 2023

Background

Coronavirus disease 2019 makes patients more susceptible to superinfection of fungal disease as a consequence of immunological system impairment. Mucormycosis is a fungal infection that is rare but has a high mortality rate and mostly affects patients with poorly controlled diabetes mellitus or those receiving corticosteroids.

Case presentation

Here, we present a case of post-coronavirus disease 2019 mucormycosis in a 37-year-old Persian male presenting with multiple periodontal abscess with purulent discharge and necrosis of maxillary bone (without oroantral communication). Surgical debridement following antifungal therapy was the treatment of choice.

Conclusion

Early diagnosis and immediate referral are the cornerstone of comprehensive treatment.
Modeling drug-induced liver injury and screening for anti-hepatofibrotic compounds using human PSC-derived organoids
Authors: Wu Xiaoshan , Jiang Dacheng , Yang Yi , Li Shuang , Ding Qiurong
Published: March 2023

Preclinical models that can accurately predict the toxicity and efficacy of candidate drugs to human liver tissue are in urgent need. Human liver organoid (HLO) derived from human pluripotent stem cells offers a possible solution. Herein, we generated HLOs, and demonstrated the utility of these HLOs in modeling a diversity of phenotypes associated with drug-induced liver injury (DILI), including steatosis, fibrosis, and immune responses. Phenotypic changes in HLOs after treatment with tool compounds such as acetaminophen, fialuridine, methotrexate, or TAK-875 showed high concordance with human clinical data in drug safety testings. Moreover, HLOs were able to model liver fibrogenesis induced by TGFβ or LPS treatment. We further devised a high-content analysis system, and established a high-throughput anti-fibrosis drug screening system using HLOs. SD208 and Imatinib were identified that can significantly suppress fibrogenesis induced by TGFβ, LPS, or methotrexate. Taken together, our studies demonstrated the potential applications of HLOs in drug safety testing and anti-fibrotic drug screening.
VMD and self-attention mechanism-based Bi-LSTM model for fault detection of optical fiber composite submarine cables
Authors: Lu Jie , Feng Wenjiang , Li Yuan , Zhang Juntao , Zou Yongqi , Li Jingfu
Published: March 2023

As the main electrical equipment of offshore power grids, optical fiber composite submarine cables undertake the task of power transmission and data communication. In order to ensure the proper functioning of the submarine cable, it is necessary to analyze the working state of it and identify the fault event. This paper proposes a fault detection method for submarine cables, that is, the VMD and self-attention-based Bi-LSTM model. First, we use ANSYS software to generate the vibration waveforms of three main fault events of optical fiber composite submarine cables. Then, by generating the detection matrix of background noise and the vibration waveforms, it can realize the orientation and detection of fault events in single submarine cable. In addition, the vibration signal can be decomposed into IMF components using variational mode decomposition (VMD) for feature extraction. Moreover, the IMF components are input to the self-attention layer for feature fusion and Bi-LSTM module for further feature extraction. Finally, the result of the fault detection is output through the classification layer. According to the comparative experiment and the ablation experiment, the proposed model has proved to outperform the other benchmark models and is robust and stable under the condition of different signal-to-noise ratios.
Maternal effects drive intestinal development beginning in the embryonic period on the basis of maternal immune and microbial transfer in chickens
Authors: Gong Haizhou , Wang Taiping , Wu Min , Chu Qianran , Lan Hainan , Lang Wuying , Zhu Lingyu , Song Yang , Zhou Yujie , Wen Qiongyi , Yu Jing , Wang Baolin , Zheng Xin
Published: March 2023

Background

Nutrition drives immunity and health in animals, and maternal immunity benefits offspring. In our previous study, a nutritional intervention strategy was found to promote the immunity of hens, which subsequently improved immunity and growth in offspring chicks. Maternal effects clearly exist, but how are mothers’ immune advantages transferred to their offspring, and how do they benefit them?

Results

Here, we traced the beneficial effects back to the process of egg formation in the reproductive system, and we focused on the embryonic intestinal transcriptome and development, as well as on maternal microbial transfer in offspring. We found that maternal nutritional intervention benefits maternal immunity, egg hatching, and offspring growth. The results of protein and gene quantitative assays showed that the transfer of immune factors into egg whites and yolks depends on maternal levels. Histological observations indicated that the promotion of offspring intestinal development begins in the embryonic period. Microbiota analyses suggested that maternal microbes transfer to the embryonic gut from the magnum to the egg white. Transcriptome analyses revealed that offspring embryonic intestinal transcriptome shifts are related to development and immunity. Moreover, correlation analyses showed that the embryonic gut microbiota is correlated with the intestinal transcriptome and development.

Conclusions

This study suggests that maternal immunity positively influences offspring intestinal immunity establishment and intestinal development beginning in the embryonic period. Adaptive maternal effects might be accomplished via the transfer of relatively large amounts of maternal immune factors and by shaping of the reproductive system microbiota by strong maternal immunity. Moreover, reproductive system microbes may be useful resources for the promotion of animal health.

Graphical Abstract
Mental time travel ability influences the representation of events and emotional expressions: evidence from microblogs
Authors: Sun Zaoyi , Li Qingyan , Luo Fei , Xu Liang
Published: March 2023

Mental time travel (MTT) ability allows people to project themselves mentally into the past and future. It is associated with people’s mental representation of events and objects. Using text analysis methods, we explore the linguistic representation and emotional expression of people with various MTT abilities. In Study 1, we assessed the users’ MTT distances, text lengths, visual perspectives, priming effects of temporal words, and emotional valences by analyzing 2973 users’ microblog texts. From our statistical analysis findings, users with far MTT incorporated longer text length and more third-person pronouns in their microblogs and are more likely to relate the future and past with the present than people with near MTT. However, the study showed no significant difference in emotional valence between people with different MTT distances. In Study 2, we explored the relationship between emotional valence and MTT ability by analyzing the comments of 1112 users on “procrastination.” We found the users with far MTT more positive toward procrastination than those with near MTT. By analyzing users’ social media platform data, this study re-examined and verified previous findings indicating that users who mentally travel different temporal distances represent events and emotional expressions differently. This study serves as an important reference for MTT studies.
The relationship of childbirth experience with postpartum depression and anxiety: a cross-sectional study
Authors: Ahmadpour Parivash , Faroughi Farnaz , Mirghafourvand Mojgan
Published: March 2023

Background

The childbirth experience is a personal life event that is influenced by physiologic and mental-psychological processes. Due to the prevalence of psychiatric problems after childbirth, it is important to recognize the factors affecting women’s emotional reactions. This study was conducted to define the relationship of childbirth experience with postpartum anxiety and depression.

Methods

This cross-sectional study was conducted on 399 women from 1 to 4 months after their childbirth who were referred to health centers in Tabriz-Iran from January 2021 to September 2021. Socio-demographic and obstetric characteristics questionnaire, Childbirth Experience Questionnaire (CEQ 2.0), Edinburgh Postpartum Depression Scale (EPDS), and Postpartum Specific Anxiety Scale (PSAS) were used to collect the data. The general linear modeling was used along with adjustment of socio-demographic characteristics to determine the relationship between the childbirth experience with depression and anxiety.

Results

The mean (SD) of the overall score for childbirth experience, anxiety, and depression were 2.9 (0.2) (score range: 1 to 4), 91.6 (4.8) (score range: 0 to153), and 9.4 (0.7) (score range: 0 to 30), respectively. There was a significant inverse correlation between the overall score of childbirth experiences, the depression score (r= -0.36, p < 0.001), and the anxiety score (r= -0.12, p = 0.028) based on the Pearson correlation test. According to the general linear modeling and with adjustment of socio-demographic characteristics, with the increasing score of the childbirth experience, the depression score decreased (B= -0.2; 95%CI: -0.3 to -0.1). Moreover, the variable of control during pregnancy was a predictor for postpartum depression and anxiety, so in women with the control during pregnancy, the mean score of postpartum depression (B= -1.8; CI 95%: -3.0 to -0.5; P = 0.004) and anxiety (B=-6.0; CI 95%: -10.1 to -1.6; P = 0.007) was less.

Conclusion

Based on the study results, postpartum depression and anxiety are related to childbirth experiences, therefore considering the effects of mothers’ mental health on other aspects of a woman and her family’s life, the core role of health care providers and policymakers in creating positive childbirth experiences is determined.
A look at the blind Kumamoto experiment: combining active and passive seismic observations to avoid Rayleigh-wave mode misidentification
Authors: Diego Mercerat E. , Dylan Mikesell T.
Published: March 2023

We present our pathway through participation in the blind Kumamoto exercise, particularly the Step 1 of site characterization. The combination of passive and active seismic imaging techniques is used to image the velocity profile beneath the KUMA site. The estimation of the broadband Rayleigh wave dispersion curve is based on cross-correlations of ambient seismic noise and analysis of active seismic shots. We calculate correlations from the entire time series (only vertical components) of each seismic array after classical pre-processing of ambient noise data. Then, a passive seismic section is constructed using all available stations pairs and stacking the cross-correlation traces with similar interstation distances. The obtained passive seismic section is analyzed using a high-resolution Radon transform to obtain the dispersion image of Rayleigh waves traveling through the KUM-LL, KUM-M and KUM-SM arrays. Then, the information is merged and interpolated to obtain the final broadband dispersion curve. In addition, active source seismic data are used with the high-resolution Radon technique to constrain the model at shallow depths (< 30 m). Then, a broadband dispersion image is constructed with significant energy from 0.9 Hz to 45 Hz. The final dispersion curve is inverted using the non-linear neighborhood algorithm. Using just the fundamental mode Rayleigh wave, a first model with normal velocity variation in depth is obtained that corresponds well with the preferred model provided by the organizing committee. The addition of a mHVSR curve in a joint inversion better constrains the deeper part of the model (> 1 km). After comparison of the submitted dispersion curve to the theoretical dispersion curve for the preferred model (Step 4 of the blind test), the authors note that there was a clear misinterpretation in the fundamental mode of their submitted results, especially at frequencies higher than 5 Hz. Using both fundamental (only visible in the passive data set) and first overtones of Rayleigh waves (only visible in the active seismic data set) a refined velocity model could have been inferred, but we decided to keep our first submitted result. This detailed interpretation should be further studied as dispersion images from forward and backward hammer shots are quite different, which may indicate strong variations in the geometry and/or shear-wave velocities of the first meters of the subsurface.

Graphical Abstract
Fault geometry of M6-class normal-faulting earthquakes in the outer trench slope of Japan Trench from ocean bottom seismograph observations
Authors: Obana Koichiro , Takahashi Tsutomu , Yamamoto Yojiro , Iinuma Takeshi , Nakamura Yasuyuki , Fujie Gou , Miura Seiichi , Kodaira Shuichi
Published: March 2023

Since the 2011 Mw 9.0 Tohoku-oki earthquake, intra-plate normal-faulting earthquakes, including several M7-class earthquakes, have occurred in the outer trench slope area from the trench to the outer rise along the Japan Trench. Concerns regarding large earthquakes and associated tsunamis have also arisen. Based on aftershock distributions, several outer trench slope normal-faulting earthquakes (hereinafter referred to as outer-rise earthquakes) are likely related to the rupture of multiple faults. However, few observations have clearly shown how multiple faults act during outer-rise earthquakes. During the ocean bottom seismograph (OBS) observations in the outer trench slope area of the central Japan Trench from September 2017 to July 2018, three M6-class normal-faulting earthquakes (Mw 6.2 on September 20, Mw 6.2 on October 06, and Mw 6.0 on November 12) occurred around the OBS network. The near-field OBS observations provided detailed information on hypocenter locations and focal mechanisms of the mainshocks and aftershocks, including immediately after the mainshocks. We investigated the fault configurations of normal-faulting earthquakes based on OBS observations. During the September 2017 earthquake, the mainshock ruptured high-angle normal faults with a dip angle of 65°. Off-fault aftershock activities that were not directly related to the mainshock rupture and could be explained by the stress changes caused by the mainshock were confirmed. However, hypocenter distributions and focal mechanisms of the main and aftershocks of the October and November 2017 earthquakes suggest that the mainshock ruptured multiple faults with various dipping directions, angles, and strike orientations. The complicated fault geometry should be considered a possible fault model for large outer-rise earthquakes and related tsunamis.
Measuring cognitive load of digital interface combining event-related potential and BubbleView
Authors: Wei Shaoyu , Zheng Ruiling , Li Rui , Shi Minghui , Zhang Junsong
Published: March 2023

Helmet mounted display systems (HMDs) are high-performance display devices for modern aircraft. We propose a novel method combining event-related potentials (ERPs) and BubbleView to measure cognitive load under different HMD interfaces. The distribution of the subjects’ attention resources is reflected by analyzing the BubbleView, and the input of the subjects’ attention resources on the interface is reflected by analyzing the ERP’s P3b and P2 components. The results showed that the HMD interface with more symmetry and a simple layout had less cognitive load, and subjects paid more attention to the upper portion of the interface. Combining the experimental data of ERP and BubbleView, we can obtain a more comprehensive, objective, and reliable HMD interface evaluation result. This approach has significant implications for the design of digital interfaces and can be utilized for the iterative evaluation of HMD interfaces.
Development and feasibility of first- and third-person motor imagery for people with stroke living in the community
Authors: Welage Nandana , Bissett Michelle , Coxon Kristy , Fong Kenneth N. K. , Liu Karen P. Y.
Published: March 2023

Background

Impairment of arm movement occurs in up to 85% of people post-stroke, affecting daily living activities, and quality of life. Mental imagery effectively enhances hand and daily function in people with stroke. Imagery can be performed when people imagine themselves completing the movement or imagine another person doing it. However, there is no report on the specific use of first-person and third-person imagery in stroke rehabilitation.

Aims

To develop and assess the feasibility of the First-Person Mental Imagery (FPMI) and the Third-Person Mental Imagery (TPMI) programs to address the hand function of people with stroke living in the community.

Methods

This study comprises phase 1—development of the FPMI and TPMI programs, and phase 2—pilot-testing of the intervention programs. The two programs were developed from existing literature and reviewed by an expert panel. Six participants with stroke, living in the community, participated in the pilot-testing of the FPMI and TPMI programs for 2 weeks. Feedback collected included the suitability of the eligibility criteria, therapist’s and participant’s adherence to intervention and instructions, appropriateness of the outcome measures, and completion of the intervention sessions within the specified time.

Results

The FPMI and TPMI programs were developed based on previously established programs and included 12 hand tasks. The participants completed four 45-min sessions in 2 weeks. The treating therapist adhered to the program protocol and completed all the steps within the specified time frame. All hand tasks were suitable for adults with stroke. Participants followed the instructions given and engaged in imagery. The outcome measures selected were appropriate for the participants. Both programs showed a positive trend towards improvement in participants’ upper extremity and hand function and self-perceived performance in activities of daily living.

Conclusions

The study provides preliminary evidence that these programs and outcome measures are feasible for implementation with adults with stroke living in the community. This study outlines a realistic plan for future trials in relation to participant recruitment, training of therapists on the intervention delivery, and the use of outcome measures.

Trial registration

Title: Effectiveness of first-person and third-person motor imagery in relearning daily hand tasks for people with chronic stroke: a randomised controlled trial.

Registration No: SLCTR/2017/031. Date registered: 22nd September 2017.