bluuebunny/crossref_metadata_2025 huggingface dataset:
Leymus chinensis Adapts to Degraded Soil Environments by Changing Metabolic Pathways and Root Exudate Components
Authors: Lin Yulong , Zhang Pan , Wu Qingying , Zhang Ying , Wei Qianhao , Sun Yihang , Wu Yuchen , Sun Shixuan , Cui Guowen
Published: May 2022

Phytoremediation is a promising remediation strategy for degraded soil restoration. Root exudates are the main carrier substances for information communication and energy transfer between plant roots and soil, which play non-negligible roles in the restoration process. This work investigated the adaptation of Leymus chinensis root exudates to different degraded levels of soil and the mechanism of rhizosphere restoration in a 3-year degraded soil field study. We found that the soil quality at each degradation level significantly increased, with the soil organic matter (SOM) content slightly increasing by 1.82%, moderately increasing by 3.27%, and severely increasing by 3.59%, and there were significant increases in the contents of available nutrients such as available phosphorus (AP), ammonia nitrogen (AN), and nitrate nitrogen (NN). The physiological activities indicated that root tissue cells also mobilize oxidative stress to respond to the soil environment pressure. A total of 473 main components were obtained from root exudates by gas chromatography–time-of-flight mass spectrometry (GC–TOFMS), including acids, alcohols, carbohydrates, and other major primary metabolites. OPLS-DA revealed that soil degradation exerted an important influence on the metabolic characteristics of root exudates, and the numbers of both up- and downregulated metabolic characteristic peaks increased with the increase in the degree of degradation. Forty-three metabolites underwent clear changes, including some defense-related metabolites and osmotic adjustment substances that were significantly changed. These changes mainly mobilized a series of lipid metabolism pathways to maintain the fluidity of membrane function and help plants adapt to unfavorable soil environmental conditions. The PPP energy metabolism pathway was mobilized in response to slight degradation, and TCA energy pathways responded to the environmental pressure of severe soil degradation.
Characterization of Three SEPALLATA-Like MADS-Box Genes Associated With Floral Development in Paphiopedilum henryanum (Orchidaceae)
Authors: Cheng Hao , Xie Xiulan , Ren Maozhi , Yang Shuhua , Zhao Xin , Mahna Nasser , Liu Yi , Xu Yufeng , Xiang Yukai , Chai Hua , Zheng Liang , Ge Hong , Jia Ruidong
Published: May 2022

Paphiopedilum(Orchidaceae) is one of the world’s most popular orchids that is found in tropical and subtropical forests and has an enormous ornamental value.SEPALLATA-like (SEP-like) MADS-box genes are responsible for floral organ specification. In this study, threeSEP-like MADS-box genes,PhSEP1,PhSEP2, andPhSEP3, were identified inPaphiopedilum henryanum. These genes were 732–916 bp, with conserved SEPI and SEPII motifs. Phylogenetic analysis revealed thatPhSEPgenes were evolutionarily closer to the core eudicotSEP3lineage, whereas none of them belonged to core eudicotSEP1/2/4clades.PhSEPgenes displayed non-ubiquitous expression, which was detectable across all floral organs at all developmental stages of the flower buds. Furthermore, subcellular localization experiments revealed the localization of PhSEP proteins in the nucleus. Yeast two-hybrid assays revealed no self-activation of PhSEPs. The protein–protein interactions revealed that PhSEPs possibly interact with B-class DEFICIENS-like and E-class MADS-box proteins. Our study suggests that the threeSEP-like genes may play key roles in flower development inP. henryanum, which will improve our understanding of the roles of theSEP-like MADS-box gene family and provide crucial insights into the mechanisms underlying floral development in orchids.
Mental Health and Social Connectedness During the COVID-19 Pandemic: An Analysis of Sports and E-Sports Players
Authors: Soares Ana Karla Silva , Goedert Maria Celina Ferreira , Vargas Adriano Ferreira
Published: May 2022

Recently, the pandemic context in which the world finds itself has inspired studies that sought to evaluate to mental health and the way people are relating to the purpose of understanding and promoting improvements psychological health. The epidemiological and public health literature shows that social connection protects and promotes mental health, being an important clinical tool for reducing anxiety, depression, and stress. Thinking in the broad sense of connection, that is, feeling and perceiving oneself connected with the environment, applied to the context of sport, it is suggested that social connection could be related to the interactions in the practice of sport. Although playing sports can promote mental health, there are few findings on the topic in the context of a pandemic and with physical sports and electronic sports (e-sports) players. In this sense, the present study aims to assess the extent to which social connection and mental health indicators are correlated in a sample of sports and e-sports players. The participants were 401 Brazilian physical sports (N= 199, 49.6%) and e-sports players (N= 202, 50.4%), mostly male (53.1%) and single (59.9%), who filled in the Social Connectedness Scale (SCS), the Depression, Anxiety and Stress Scale (DASS), and demographic questions. The results indicated that social connection was negatively correlated and also predict the anxiety (r= −0.37), depression (r= −0.54), and stress (r= −0.39). When comparing sports and e-sports players, a statistically significant difference was identified in the levels of social connection [t(398) = −3.41; sportsmean(SD) = 4.53 (1.14); e-sportsmean(SD) = 4.14 (1.15)] and depression [t(396) = 2.90; sportsmean(SD) = 1.10 (0.89); e-sportsmean(SD) = 0.85 (0.81)]. These findings can serve as a theoretical basis for the development of intervention programs (e.g., to guide managers regarding the social distancing rules that enable them to keep holding sports practices and events) and promoting discussions that focus on the analysis of aspects promoting psychological health in sports context (physical and e-sports).
Construction of Intelligent Recognition and Learning Education Platform of National Music Genre Under Deep Learning
Authors: Xu Zhongkui
Published: May 2022

In order to study the application of the deep learning (DL) method in music genre recognition, this study introduces the music feature extraction method and the deep belief network (DBN) in DL and proposes the parameter extraction feature and the recognition classification method of an ethnic music genre based on the DBN with five kinds of ethnic musical instruments as the experimental objects. A national musical instrument recognition and classification network structure based on the DBN is proposed. On this basis, a music library classification retrieval learning platform has been established and tested. The results show that, when the DBN only contains one hidden layer and the number of neural nodes in the hidden layer is 117, the basic convergence accuracy is approximately 98%. The first hidden layer has the greatest impact on the prediction results. When the input sample feature size is one-third of the number of nodes in the first hidden layer, the network performance is basically convergent. The DBN is the best way for softmax to identify and classify national musical instruments, and the accuracy rate is 99.2%. Therefore, the proposed DL algorithm performs better in identifying music genres.
Perceived Dignity of Advanced Cancer Patients and Its Relationship to Sociodemographic, Clinical, and Psychological Factors
Authors: Obispo Berta , Cruz-Castellanos Patricia , Hernandez Raquel , Gil-Raga Mireia , González-Moya Manuel , Rogado Jacobo , López-Ceballos Helena , García-Carrasco Miguel , Jiménez-Fonseca Paula , Calderon Caterina
Published: May 2022

Objective

Loss of dignity is one of the main reasons for wishing for an early death in patients with incurable diseases such as cancer and is strongly associated with psychological distress and loss of quality of life. The present study aims to analyze the perceived dignity of patients with advanced cancer undergoing systemic treatment and their relationship with sociodemographic, clinical, and psychological factors.

Methods

A prospective, cross-sectional, multicenter study was conducted in 15 oncology departments in Spain. Patients with locally advanced, unresectable, or metastatic cancer who were candidates for systemic treatment were included. Participants completed demographic information and Palliative Patients’ Dignity Scale, Brief Symptom Inventory, Mental Adjustment to Cancer, Functional Social Support Questionnaire, and Illness Uncertainty.

Results

A total of 508 patients were recruited between February 2020 and October 2021. Most were male, aged > 65 years, with digestive tumors (41%), and metastatic disease at diagnosis. Subjects were classified as having low (56%, N = 283) or high (44%, N = 225) perceived dignity. Patients ≥ 65 years, with worse baseline status (ECOG ≥ 1), and worse estimated 18-month survival had lower levels of perceived dignity. People with lower perceived dignity scored higher for anxious preoccupation and hopelessness and lower for positive attitude. They also displayed higher levels of anxiety, depression, and somatic symptoms, greater uncertainty, and less social support.

Conclusion

Self-perceived dignity in advancer cancer patients is significantly associated with psychological factors, psychological distress, uncertainty, less social support. Knowledge of these specific interactions is importance for adequate, comprehensive palliative care.
Testing the Magnitude of Correlations Across Experimental Conditions
Authors: Di Plinio Simone
Published: May 2022

Correlation coefficients are often compared to investigate data across multiple research fields, as they allow investigators to determine different degrees of correlation to independent variables. Even with adequate sample size, such differences may be minor but still scientifically relevant. To date, although much effort has gone into developing methods for estimating differences across correlation coefficients, adequate tools for variable sample sizes and correlational strengths have yet to be tested. The present study evaluated four different methods for detecting the difference between two correlations and tested the adequacy of each method using simulations with multiple data structures. The methods tested were Cohen’s q, Fisher’s method, linear mixed-effects models (LMEM), and an ad hoc developed procedure that integrates bootstrap and effect size estimation. Correlation strengths and sample size was varied across a wide range of simulations to test the power of the methods to reject the null hypothesis (i.e., the two correlations are equal). Results showed that Fisher’s method and the LMEM failed to reject the null hypothesis even in the presence of relevant differences between correlations and that Cohen’s method was not sensitive to the data structure. Bootstrap followed by effect size estimation resulted in a fair, unbiased compromise for estimating quantitative differences between statistical associations and producing outputs that could be easily compared across studies. This unbiased method is easily implementable in MatLab through the bootes function, which was made available online by the author at MathWorks.
Binge Eating and Compulsive Buying During Cabergoline Treatment for Prolactinoma: A Case Report
Authors: Correa e Castro Ana Carolina , de Araujo Andressa Alexandre , Botelho Mariana Coelho , Nascimento João Bosco , de Souza Rafaela Marchon , Gadelha Monica Roberto , Nardi Antonio E. , Violante Alice Helena Dutra
Published: May 2022

Prolactinomas are the most prevalent functional pituitary adenomas. They are usually treated clinically with dopamine agonists. The most widely used and suitable drug is cabergoline (CAB), a specific D2 dopamine agonists. Patients in prolactinoma treatment with CAB commonly report physical side effects, but aberrant behavioral changes such as increased impulsivity have also been reported recently. We report the case of a 47-year-old Brazilian woman with prolactinoma that developed compulsive buying, binge eating, and hypersexuality after four years of CAB treatment. In her psychiatric evaluation, the patient scored high levels on the following scales: Compulsive Buying Scale (CBS), Binge Eating Scale (BES), and Barratt Impulsiveness Scale-11 (BIS11). She also reported financial problems and weight gain in addition to her social and clinical problems. Impulsivity disorders may appear with the use of CAB and other dopamine agonists. We suggest that more observational studies with a large patient sample and specific regular psychiatric evaluations during treatment are necessary for patients in use of CAB, especially those treated for several years.
Validity Evaluation of the College Student Physical Literacy Questionnaire
Authors: Luo Lin , Song Naiqing , Huang Jin , Zou Xiaodong , Yuan Junfeng , Li Chenli , Yang Jie , Zhou Ling , Zhang Liping , Luo Shiyan , Gao Xin
Published: May 2022

Background

Physical literacy (PL) is an important tool to promote physical activity of individuals, and the level of physical literacy of individuals affects their physical activity behaviors. Currently, the physical fitness of college students in China is a prominent issue, and assessing physical literacy among college students may provide tools and directions to further promote physical fitness and precisely intervene in physical activity behaviors of college students in the future. This study aimed to develop a college student physical literacy questionnaire (CSPLQ) to address the lack of currently available physical literacy assessment tools for Chinese college students. We hoped to collect validity evidence of this questionnaire to measure the validity of the physical literacy self-assessment questionnaire among Chinese university students.

Methods

An initial pool of items was obtained from existing research instruments, literature, and expert advice. An expert review panel evaluated its content. A subsequent validation process reduced the pool of items. We conducted a validation factor analysis of the CSPLQ using structural equation modeling. The relationship between physical literacy and other variables was also examined using correlation analysis.

Results

The item content validity index (ICVI) of CSPLQ was 0.70–0.95. The CSPLQ was composed of a total of 38 items across 3 domains (physical and behavioral domain, affective domain, and cognitive domain) and 7 dimensions (motor skills, motor skills, physical activity, perceptions of healthy living, perceptions of physical activity, motivation to engage in physical activity, and confidence to engage in physical activity). The factor validity of the CSPLQ was determined by significant loading of all items on their expected factors, with good data model fit and good stability between two independent samples were demonstrated. Each subscale had a Cronbach α coefficient >0.9 and was strongly correlated with each other. The correlation coefficients between college students' physical literacy and other variables, including athletic ability, physical condition, physical attractiveness, physical fitness, frequency of physical activity, and length of physical activity, all reached a significance level of P < 0.05.

Conclusion

The CSPLQ has sufficient evidence of validity. The development of the instrument showed evidence of validity for the content, response process, internal structure, and relationships with other variables.
Access Barriers to Dental Treatment and Prevention for Turkish Migrants in Germany – A Qualitative Survey
Authors: Spinler Kristin , Kofahl Christopher , Ungoreit Erik , Heydecke Guido , Dingoyan Demet , Aarabi Ghazal
Published: May 2022

Introduction

The worldwide migration movement is growing and thereby challenging the health care systems of immigration countries like Germany to make health care equally accessible for all people. Due to their low oral health status and low uptake rates of dental treatment and prevention, migrants were detected as a vulnerable group. Data regarding dental care access barriers of this group is limited. Therefore, the following study established a deeper understanding of unknown access barriers.

Methods

Nine expert interviews and one focus group interview were conducted semi-structured via interview guideline in the period of August until October 2018. The experts were persons with strong vocational interactions and experiences with the sector oral health care and migration. The focus group participants had a Turkish migration background.

Results

The expert and focus group interviews revealed a variety of barriers that exist toward dental treatment and prevention for migrants. Language, perceived significance of oral health, oral health knowledge, health socialization and patient-dentist interaction were detected to be the main barriers with underlying subthemes and interactions. Furthermore, a predominantly not precaution-oriented dental service utilization of migrants was underlined by the interviewees. Additionally, ways to reach a higher cultural sensitivity in oral health care were stated.

Conclusion

With respect for research, there is a need for the integration of migrant-specific items when collecting health data from people. With respect for policy, there is a need for more structural and individual attention for promoting equal access to oral health care and prevention measures for people with a migrant background.
Prediction Performance Comparison of Risk Management and Control Mode in Regional Sites Based on Decision Tree and Neural Network
Authors: Zhu Wenhui , He Jun , Zhang Hongzhen , Cheng Liang , Yang Xintong , Wang Xiahui , Ji Guohua
Published: May 2022

The traditional risk management and control mode (RMCM) in regional sites has the defects of low efficiency, high cost, and lack of systematism. Trying to resolve these defects and explore the application possibility of machine learning, a characteristic dataset for RMCM in regional sites was established. Three decision tree (DT) algorithms (CHAID, EXHAUSTIVE CHAID, and CART) and two artificial neural network (ANN) algorithms [back propagation (BP) and radial basis function (RBF)] were implemented to predict RMCM in regional sites. The results showed that in the aspects of accuracy (ACC), precision (PRE), recall ratio (REC), and F1 value, CART–DT was superior to CHAID–DT and EXHAUSTIVE CHAID–DT (E-CHAID–DT); and BP–ANN was superior to RBF–ANN. However, CART–DT was inferior to BP–ANN in ACC, PRE, REC, and F1 value. BP–ANN model is good at non-linear mapping, and it has a flexible network structure and a low risk of over-fitting. The case study of a typical county demonstration area confirmed the extensibility of the method, and the method has great potential in RMCM prediction in regional sites in the future.
Applying Theory to Explain the Influence of Factors External to an Organization on the Implementation of an Evidence-Based Intervention
Authors: Leeman Jennifer , Wangen Mary , Kegler Michelle , Lee Matthew , O'Leary Meghan C. , Ko Linda K. , Fernández María E. , Birken Sarah A.
Published: May 2022

Despite its widely acknowledged influence on implementation, limited research has been done on how the external environment (i.e., outer setting) determines when organizations adopt and implement new interventions. Determinant frameworks identify several outer setting-level factors such as funding streams, inter-organizational relationships, and peer pressure. However, these frameworks do not explain how or why outer-setting factors influence implementation. To advance research in this area, we argue for the importance of deriving theory-based propositions from organization theory to explain how outer setting factors influence organizations. Drawing on the work of the Organization Theory in Implementation Science (OTIS) project, we identified 20 propositions from five classic organization theories—Complexity Theory, Contingency Theory, Institutional Theory, Resource Dependence Theory, and Transaction Cost Economics. We then applied those propositions to hypothesize relationships among outer setting factors, implementation strategies, and implementation outcomes in five case studies of evidenced-based tobacco control interventions. The five case studies address the implementation of smoke-free policies, community health worker-led tobacco education and cessation programs, 5 A's (Ask, Advise, Assess, Assist, and Arrange), point-of-sale tobacco marketing policy interventions, and quitlines. The case studies illustrate how propositions may be used to guide the selection and testing of implementation strategies. Organization theories provide a menu of propositions that offer guidance for selecting and optimizing high-leverage implementation strategies that target factors at the level of outer setting. Furthermore, these propositions suggest testable hypotheses regarding the mechanisms underlying the influence of outer-setting factors on how and why organizations adopt and implement interventions.
DRIS Norms and Sufficiency Ranges for Persimmon ‘Rojo Brillante’ Grown under Mediterranean Conditions in Spain
Authors: Morales Julia , Rodríguez-Carretero Isabel , Martínez-Alcántara Belen , Canet Rodolfo , Quiñones Ana
Published: May 2022

The aim of this study was to establish DRIS (Diagnosis and Recommendation Integrated System) norms and Nutritional Optimal Ranges (NOR) for ‘Rojo Brillante’ Protected Designation of Origin (PDO) ‘Ribera del Xúquer’. The database contained 800 leaf samples collected in different crop phenological stages [after flowering (AF), fruit enlargement (FE), fruit colouring (FC), and harvesting HV)]. DRIS norms (78) were established for macronutrients: N, P, K, Ca, Mg and S; micronutrients B, Cu, Fe, Mn, and Zn and salinity elements: Na and Cl. The Nutrient Balance Index (NBI; the absolute value of the sum of the DRIS indices) was used to determine the optimal sampling period. Fruit enlargement was the period during which persimmon trees were more nutritionally balanced regardless of sprout origin (vegetative or floral) and irrigation type (drip or flood) in orchards
Soil Waterlogging Conditions Affect Growth, Water Status, and Chlorophyll “a” Fluorescence in Coffee Plants (Coffea arabica L.)
Authors: León-Burgos Andrés Felipe , Unigarro Carlos Andrés , Balaguera-López Helber Enrique
Published: May 2022

Soil waterlogging is an environmental limitation that is promoted by changes in rainfall patterns and negatively affects plant physiology, growth, and development, coffee production, and exports in Colombia. The objective of this research was to evaluate responses in growth, water status, and chlorophyll fluorescence parameters for plants under different waterlogging conditions. The evaluations were carried out on six-month-old “Cenicafé 1” variety seedlings that were suitable for establishment in the field. The seedlings were subjected to different waterlogging periods: 4, 8, 12, and 16 days, using a completely randomized block experiment design with 30 replicates. Total dry mass (TDM), total leaf area (TLA), and the total number of leaves (TNL) were performed, and growth indices were calculated. The leaf water potential (Ψpd and Ψmd), maximum efficiency of photosystem II-FV/Fm, and electron transport rate (ETR) were also measured. Waterlogging for 16 days caused a 57% reduction for TDM, which was significant starting at 4 days. Statistical differences in the TLA were detected after 12 days, with decreases of 29%. The Ψpd, Ψmd, Fv/Fm, and ETR were sensitive to waterlogging, which decreased noticeably after 8 days. The results indicated the physiological performance and growth of the coffee cv. “Cenicafé 1” plants were significantly affected by waterlogging conditions after eight days.
Population Genetic Structure and Geometric Morphology of Codling Moth Populations from Different Management Systems
Authors: Balaško Martina Kadoić , Bažok Renata , Mikac Katarina M. , Benítez Hugo A. , Suazo Manuel J. , Viana João Paulo Gomes , Lemic Darija , Živković Ivana Pajač
Published: May 2022

Codling moth (CM), Cydia pomonella L., is an important pest of apples worldwide. CM resistance to insecticides is a serious problem in apple production. For effective management and control, monitoring of resistant CM populations is absolutely necessary. Therefore, in this study, we investigated whether it is possible to find a reliable pattern of differences in CM populations related to the type of apple control method. The genetic results showed low estimated value of the pairwise fixation index, FST = 0.021, which indicates a lack of genetic differentiation and structuring between the genotyped populations. Different approaches were used to analyze the genetic structure of codling moth populations: Bayesian-based model of population structure (STRUCTURE), principal component analysis (PCA), and discriminant analysis of principal components (DAPC). STRUCTURE grouped the CM genotypes into two distinct clusters, and the results of PCA were consistent with this. The DAPC revealed three distinct groups. However, the results showed that population genetic differentiation between organic and integrated orchards was not significant. To confirm the genetic results, the forewing morphology of the same CM individuals was examined using geometric morphometric techniques based on the venation patterns of 18 landmarks. The geometric results showed higher sensitivity and separated three distinct groups. Geometric morphometrics was shown to be a more sensitive method to detect variability in genotypes due to pest control management. This study shows the possibility of using a novel method for a strategic integrated pest management (IPM) program for CM that is lacking in Europe.
Occurrence of blaNDM-1-Positive Providencia spp. in a Pig Farm of China
Authors: Chen Wenxin , Liu Zhihong , Lin Hongguang , Yang Jie , Liu Ting , Zheng Jiaomei , Long Xueming , Sun Zhiliang , Li Jiyun , Chen Xiaojun
Published: May 2022

Antibiotics have been extensively used to ensure the productivity of animals on intensive livestock farms. Accordingly, antimicrobial-resistant organisms, which can be transmitted to humans via the food chain, pose a threat to public health. The Enterobacterium antimicrobial resistance gene, blaNDM-1, is a transmissible gene that has attracted widespread attention. Here, we aimed to investigate the prevalence of Enterobacteriaceae carrying blaNDM-1 on an intensive pig farm. A total of 190 samples were collected from a pig farm in Hunan Province, China. Resistant isolates were selected using MacConkey agar with meropenem and PCR to screen for blaNDM-1-positive isolates. Positive strains were tested for conjugation, antimicrobial susceptibility, and whole-genome sequencing. Four blaNDM-1-positive Providencia strains were obtained, and multidrug resistance was observed in these strains. The structure carrying blaNDM-1 did not conjugate to E. coli J53 after three repeated conjugation assays. This suggests that, in intensive farming, attention should be focused on animal health and welfare to reduce the frequency of antibiotic usage. Carbapenem-resistant Enterobacteriaceae in the breeding industry should be included in systematic monitoring programs, including animal, human, and environmental monitoring programs.
Diagnosis and Treatment of Invasive Candidiasis
Authors: Barantsevich Natalia , Barantsevich Elena
Published: May 2022

Candida species, belonging to commensal microbial communities in humans, cause opportunistic infections in individuals with impaired immunity. Pathogens encountered in more than 90% cases of invasive candidiasis include C. albicans, C. glabrata, C. krusei, C. tropicalis, and C. parapsilosis. The most frequently diagnosed invasive infection is candidemia. About 50% of candidemia cases result in deep-seated infection due to hematogenous spread. The sensitivity of blood cultures in autopsy-proven invasive candidiasis ranges from 21% to 71%. Non-cultural methods (beta-D-glucan, T2Candida assays), especially beta-D-glucan in combination with procalcitonin, appear promising in the exclusion of invasive candidiasis with high sensitivity (98%) and negative predictive value (95%). There is currently a clear deficiency in approved sensitive and precise diagnostic techniques. Omics technologies seem promising, though require further development and study. Therapeutic options for invasive candidiasis are generally limited to four classes of systemic antifungals (polyenes, antimetabolite 5-fluorocytosine, azoles, echinocandins) with the two latter being highly effective and well-tolerated and hence the most widely used. Principles and methods of treatment are discussed in this review. The emergence of pan-drug-resistant C. auris strains indicates an insufficient choice of available medications. Further surveillance, alongside the development of diagnostic and therapeutic methods, is essential.
EEG-Based Emotion Recognition Using a 2D CNN with Different Kernels
Authors: Wang Yuqi , Zhang Lijun , Xia Pan , Wang Peng , Chen Xianxiang , Du Lidong , Fang Zhen , Du Mingyan
Published: May 2022

Emotion recognition is receiving significant attention in research on health care and Human-Computer Interaction (HCI). Due to the high correlation with emotion and the capability to affect deceptive external expressions such as voices and faces, Electroencephalogram (EEG) based emotion recognition methods have been globally accepted and widely applied. Recently, great improvements have been made in the development of machine learning for EEG-based emotion detection. However, there are still some major disadvantages in previous studies. Firstly, traditional machine learning methods require extracting features manually which is time-consuming and rely heavily on human experts. Secondly, to improve the model accuracies, many researchers used user-dependent models that lack generalization and universality. Moreover, there is still room for improvement in the recognition accuracies in most studies. Therefore, to overcome these shortcomings, an EEG-based novel deep neural network is proposed for emotion classification in this article. The proposed 2D CNN uses two convolutional kernels of different sizes to extract emotion-related features along both the time direction and the spatial direction. To verify the feasibility of the proposed model, the pubic emotion dataset DEAP is used in experiments. The results show accuracies of up to 99.99% and 99.98 for arousal and valence binary classification, respectively, which are encouraging for research and applications in the emotion recognition field.
Co-Ultramicronized Palmitoylethanolamide/Luteolin Restores Oligodendrocyte Homeostasis via Peroxisome Proliferator-Activated Receptor-α in an In Vitro Model of Alzheimer’s Disease
Authors: Facchinetti Roberta , Valenza Marta , Gomiero Chiara , Mancini Giulia Federica , Steardo Luca , Campolongo Patrizia , Scuderi Caterina
Published: May 2022

Oligodendrocytes are cells fundamental for brain functions as they form the myelin sheath and feed axons. They perform these critical functions thanks to the cooperation with other glial cells, mainly astrocytes. The astrocyte/oligodendrocyte crosstalk needs numerous mediators and receptors, such as peroxisome proliferator-activated receptors (PPARs). PPAR agonists promote oligodendrocyte precursor cells (OPCs) maturation in myelinating oligodendrocytes. In the Alzheimer’s disease brain, deposition of beta-amyloid (Aβ) has been linked to several alterations, including astrogliosis and changes in OPCs maturation. However, very little is known about the molecular mechanisms. Here, we investigated for the first time the maturation of OPCs co-cultured with astrocytes in an in vitro model of Aβ1–42 toxicity. We also tested the potential beneficial effect of the anti-inflammatory and neuroprotective composite palmitoylethanolamide and luteolin (co-ultra PEALut), which is known to engage the isoform alfa of the PPARs. Our results show that Aβ1–42 triggers astrocyte reactivity and inflammation and reduces the levels of growth factors important for OPCs maturation. Oligodendrocytes indeed show low cell surface area and few arborizations. Co-ultra PEALut counteracts the Aβ1–42-induced inflammation and astrocyte reactivity preserving the morphology of co-cultured oligodendrocytes through a mechanism that in some cases involves PPAR-α. This is the first evidence of the negative effects exerted by Aβ1–42 on astrocyte/oligodendrocyte crosstalk and discloses a never-explored co-ultra PEALut ability in restoring oligodendrocyte homeostasis.
Non-Thermal Plasma Jet-Treated Medium Induces Selective Cytotoxicity against Mycobacterium tuberculosis-Infected Macrophages
Authors: Lee Chae Bok , Lee Kang In , Kim Young Jae , Jang In Taek , Gurmessa Sintayehu Kebede , Choi Eun Ha , Kaushik Nagendra Kumar , Kim Hwa-Jung
Published: May 2022

Plasma-treated media (PTM) serve as an adjuvant therapy to postoperatively remove residual cancerous lesions. We speculated that PTM could selectively kill cells infected with Mycobacterium tuberculosis (Mtb) and remove postoperative residual tuberculous lesions. We therefore investigated the effects of a medium exposed to a non-thermal plasma jet on the suppression of intracellular Mtb replication, cell death, signaling, and selectivity. We propose that PTM elevates the levels of the detoxifying enzymes, glutathione peroxidase, catalase, and ataxia-telangiectasia mutated serine/threonine kinase and increases intracellular reactive oxygen species production in Mtb-infected cells. The bacterial load was significantly decreased in spleen and lung tissues and single-cell suspensions from mice intraperitoneally injected with PTM compared with saline and untreated medium. Therefore, PTM has the potential as a novel treatment that can eliminate residual Mtb-infected cells after infected tissues are surgically resected.
A Machine Learning Model to Predict Knee Osteoarthritis Cartilage Volume Changes over Time Using Baseline Bone Curvature
Authors: Bonakdari Hossein , Pelletier Jean-Pierre , Abram François , Martel-Pelletier Johanne
Published: May 2022

The hallmark of osteoarthritis (OA), the most prevalent musculoskeletal disease, is the loss of cartilage. By using machine learning (ML), we aimed to assess if baseline knee bone curvature (BC) could predict cartilage volume loss (CVL) at one year, and to develop a gender-based model. BC and cartilage volume were assessed on 1246 participants using magnetic resonance imaging. Variables included age, body mass index, and baseline values of eight BC regions. The outcome consisted of CVL at one year in 12 regions. Five ML methods were evaluated. Validation demonstrated very good accuracy for both genders (R ≥ 0.78), except the medial tibial plateau for the woman. In conclusion, we demonstrated, for the first time, that knee CVL at one year could be predicted using five baseline BC region values. This would benefit patients at risk of structural progressive knee OA.
Telomere as a Therapeutic Target in Dedifferentiated Liposarcoma
Authors: Alessandrini Irene , Percio Stefano , Naghshineh Eisa , Zuco Valentina , Stacchiotti Silvia , Gronchi Alessandro , Pasquali Sandro , Zaffaroni Nadia , Folini Marco
Published: May 2022

Background: Well-differentiated (WD)/dedifferentiated (DD) liposarcoma (LPS) accounts for ~60% of retroperitoneal sarcomas. WDLPS and DDLPS divergently evolve from a common precursor and are both marked by the amplification of the 12q13–q15 region, leading to the abnormal expression of MDM2, CDK4, and HMGA2 genes. DDLPS is a non-lipogenic disease associated with aggressive clinical behavior. Patients have limited therapeutic options, especially for advanced disease, and their outcome remains largely unsatisfactory. This evidence underlines the need for identifying and validating DDLPS-specific actionable targets to design novel biology-driven therapies. Methods: Following gene expression profiling of DDLPS clinical specimens, we observed the up-regulation of “telomere maintenance” (TMM) pathways in paired DD and WD components of DDLPS. Considering the relevance of TMM for LPS onset and progression, the activity of a telomeric G-quadruplex binder (RHPS4) was assessed in DDLPS patient-derived cell lines. Results: Equitoxic concentrations of RHPS4 in DDLPS cells altered telomeric c-circle levels, induced DNA damage, and resulted in the accumulation of γ-H2AX-stained micronuclei. This evidence was paralleled by an RHPS4-mediated reduction of in vitro cell migration and induction of apoptosis/autophagy. Conclusions: Our findings support telomere as an intriguing therapeutic target in DDLPS and suggest G-quadruplex binders as innovative therapeutic agents.
Updates on Diagnostic and Therapeutic Management of Gastrointestinal and Pancreatic NET
Authors: Krug Sebastian , Schrader Jörg , Rinke Anja
Published: May 2022

Gastroenteropancreatic neuroendocrine neoplasms (GEP-NEN) comprise a plethora of distinct molecular-pathological, clinical, diagnostical and therapeutical approaches to enable individualized treatment [...]
Sensitization of Resistant Breast Cancer Cells with a Jumonji Family Histone Demethylase Inhibitor
Authors: Singh Balraj , Sarli Vanessa N. , Lucci Anthony
Published: May 2022

In the present study, we evaluated JIB-04, a small-molecule epigenetic inhibitor initially discovered to inhibit cancer growth, to determine its ability to affect deep intrinsic resistance in a breast cancer model. The model was based on a function-based approach to the selection of cancer cells in a cell culture that can survive a variety of challenges in prolonged, but reversible, quiescence. These resistant cancer cells possessed a variety of mechanisms, including modifications of the epigenome and transcriptome, for generating a high degree of cellular heterogeneity. We found that long pretreatment with JIB-04 sensitized resistant triple-negative inflammatory breast cancer cells and their parental cell line SUM149 to the chemotherapeutic drugs doxorubicin and paclitaxel. Resistant cancer cells derived from another inflammatory breast cancer cell line, FC-IBC02, were considerably more sensitive to JIB-04 than the parental cell line. Investigating a mechanism of sensitization, we found that JIB-04 exposure increased the expression of PD-L1 in resistant cells, suggesting that JIB-04 may also sensitize resistant breast cancer cells to anti-PD-L1 immune therapy. Finally, these results support the usefulness of a cell culture-based experimental strategy for evaluating anticancer agents, such as JIB-04, that may halt cancer evolution and prevent the development of cancer resistance to currently used therapies.
Enthesis Healing Is Dependent on Scaffold Interphase Morphology—Results from a Rodent Patellar Model
Authors: Peniche Silva Carlos J. , Müller Sebastian A. , Quirk Nicholas , Poh Patrina S. P. , Mayer Carla , Motta Antonella , Migliaresi Claudio , Coenen Michael J. , Evans Christopher H. , Balmayor Elizabeth R. , van Griensven Martijn
Published: May 2022

The use of multiphasic scaffolds to treat injured tendon-to-bone entheses has shown promising results in vitro. Here, we used two versions of a biphasic silk fibroin scaffold to treat an enthesis defect created in a rat patellar model in vivo. One version presented a mixed transition between the bony and the tendon end of the construct (S-MT) while this transition was abrupt in the second version (S-AT). At 12 weeks after surgery, the S-MT scaffold promoted better healing of the injured enthesis, with minimal undesired ossification of the insertion area. The expression of tenogenic and chondrogenic markers was sustained for longer in the S-MT-treated group and the tangent modulus of the S-MT-treated samples was similar to the native tissue at 12 weeks while that of the S-AT-treated enthesis was lower. Our study highlights the important role of the transition zone of multiphasic scaffolds in the treatment of complex interphase tissues such as the tendon-to-bone enthesis.
Arctic Sea Ice Decline and Geoengineering Solutions: Cascading Security and Ethical Considerations
Authors: Bennett Alec P. , Bouffard Troy J. , Bhatt Uma S.
Published: May 2022

Climate change is generating sufficient risk for nation-states and citizens throughout the Arctic to warrant potentially radical geoengineering solutions. Currently, geoengineering solutions such as surface albedo modification or aerosol deployment are in the early stages of testing and development. Due to the scale of deployments necessary to enact change, and their preliminary nature, these methods are likely to result in unforeseen consequences. These consequences may range in severity from local ecosystem impacts to large scale changes in available solar energy. The Arctic is an area that is experiencing rapid change, increased development, and exploratory interest, and proposed solutions have the potential to produce new risks to both natural and human systems. This article examines potential security and ethical considerations of geoengineering solutions in the Arctic from the perspectives of securitization, consequentialism, and risk governance approaches, and argues that proactive and preemptive frameworks at the international level, and especially the application of risk governance approaches, will be needed to prevent or limit negative consequences resulting from geoengineering efforts. Utilizing the unique structures already present in Arctic governance provides novel options for addressing these concerns from both the perspective of inclusive governance and through advancing the understanding of uncertainty analysis and precautionary principles.
Exogenous Zeaxanthin Alleviates Low Temperature Combined with Low Light Induced Photosynthesis Inhibition and Oxidative Stress in Pepper (Capsicum annuum L.) Plants
Authors: Ding Dongxia , Li Jing , Xie Jianming , Li Nenghui , Bakpa Emily Patience , Han Kangning , Yang Yan , Wang Cheng
Published: May 2022

Low temperature combined with low light (LL) affects crop production, especially the yield and quality of peppers, in northwest China during the winter and spring seasons. Zeaxanthin (Z) is a known lipid protectant and active oxygen scavenger. However, whether exogenous Z can mitigate LL-induced inhibition of photosynthesis and oxidative stress in peppers remains unclear. In this study, we investigated the effects of exogenous Z on photosynthesis and the antioxidant machinery of pepper seedlings subject to LL stress. The results showed that the growth and photosynthesis of pepper seedlings were significantly inhibited by LL stress. In addition, the antioxidant machinery was disturbed by the uneven production and elimination of reactive oxygen species (ROS), which resulted in damage to the pepper. For example, membrane lipid peroxidation increased ROS content, and so on. However, exogenous application of Z before LL stress significantly increased the plant height, stem diameter, net photosynthetic rate (Pn), and stomata, which were obviously closed at LL. The activities of antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), mono de-hydroascorbate reductase (MDHAR), de-hydroascorbate reductase (DHAR), ascorbate peroxidase (APX), and ascorbate oxidase (AAO) improved significantly due to the increased expression of CaSOD, CaCAT, CaAPX, CaMDHAR, and CaDHAR. The ascorbic (AsA) and glutathione (GSH) contents and ascorbic/dehydroascorbate (AsA/DHA) and glutathione/oxidized glutathione (GSH/GSSG) ratios also increased significantly, resulting in the effective removal of hydrogen peroxide (H2O2) and superoxide anions (O2•−) caused by LL stress. Thus, pre-treatment with Z significantly reduced ROS accumulation in pepper seedlings under LL stress by enhancing the activity of antioxidant enzymes and accumulation of components of the ascorbate–glutathione (AsA–GSH) cycle and upregulated key genes in the AsA–GSH cycle.
Formulation and Characterization of a Composite Coating Formulation Based on Acrylic Foam and Cork Granules
Authors: Monteiro Sandra , Ferreira Nuno , Paiva Diana , Silva Susana P. , Martins Jorge , Carvalho Luísa H. , Magalhães Fernão D.
Published: May 2022

Cork, the bark of Quercus suber L., in addition to presenting several notable physical-mechanical properties, possesses a distinctive look and feel that make it attractive for interior surfaces, such as in furniture, wall paneling, or flooring. This work envisaged the development of a coating based on cork granules, a subproduct from the wine stopper industry, capable of creating a smooth surface similar to natural cork. In order to avoid the high rugosity that characterizes surfaces coated with paints that incorporate cork granules, a new solution was developed, based on a foamed acrylic binder, applied by knife coating. The foam formulation was successfully optimized, using appropriate additives and resorting to mechanical agitation to promote the generation of air bubbles. The addition of cork granules did not hinder foam stability, and the final coating displayed the intended visual and sensory characteristics. Dynamic Mechanical Analysis was performed on the pristine acrylic foam and on the composite foam showed a stiffening effect associated with the presence of cork granules, and a thermal transition centered at around −10 °C, associated with the acrylic binder’s glass transition. The surface has hardness slightly lower than cork, depending on the amount of particles incorporated. Pull-off testing consistently resulted in substrate failure, indicating that the coating’s cohesion and adhesion are excellent. The developed coating showed to have the intended functionality while being easily applicable on flat panel surfaces. The fact that a foam is used as a binder system allows for a smooth and soft surface, having excellent opacity with minimal usage of cork.
In Vitro Evaluation of the Shading Effect of Various Zirconia Surface Stains on Porcelain Crowns
Authors: Lin Shih-Chieh , Lin Wei-Chun , Lin Yu-Ling , Yan Min , Tang Cheng-Ming
Published: May 2022

Human teeth display various colors under natural light. Dental restorations, such as zirconia crowns, are generally used to rehabilitate the oral function of patients with tooth loss due to trauma or natural tooth falls. However, significant improvements in the color and translucency of zirconia are required to meet the clinical needs for dental restoration. In the past, a large amount of ceramic powder has been used to improve the appearance of zirconia. However, the interface between the ceramic powder and zirconia makes them prone to falling off. Therefore, the aesthetics of zirconia crowns remains a major challenge. Recently, substantial advances have been made in the field of dental materials, as special staining agents for zirconia have been introduced as alternatives to ceramic powders. Therefore, this study tested zirconia-specific staining agents that were used to produce zirconia samples with A1 and A3 colors. A dental colorimetric plate was used as the control group to assess the staining effects of the different brands of staining agents. Meanwhile, two hypotheses were proposed: that the staining effects of these special staining agents for zirconia met the criteria for clinical application and that there was no significant difference between the different staining agents for zirconia. The results showed that the coatings of different brands of staining agents were ultrathin, with a thickness of approximately 27–78 µm. In addition, the coloring effects of the zirconia staining agents were not significantly different from those of the colorimetric plates. After staining, the zirconia samples had decreased surface roughness and contact angle values, which improved surface smoothness and cleanliness. In summary, the results support the hypothesis of this study that zirconia stains can be used as an alternative to the current fabrication methods for clinical dental restorations. We sought to identify the clinical techniques that are easier to perform and to overcome the current problem of dental technicians requiring considerable dentin space for staining. It is expected that the results of this study will be useful in clinical dental restorations.