bluuebunny/crossref_metadata_2025 huggingface dataset:
GENERAL HISTORICAL IMPERATIVES OF SOCIAL DEVELOPMENT AND RUSSIAN CIVILIZATION
Authors:   Lomonosov Moscow State University, Moscow, Russian Federation, KOVALENKO VALERIY
Published: September 2022

The article is a summary of the author’s report on May 19, 2022 at the round table “Russia and the World: between East and West” within the framework of the permanent seminar “Global Factors of Modern Public Policy” organized by the Departments of Public Policy and Russian Politics of the Faculty of Political Science of Lomonosov Moscow State University and the Academy of Political Science. The various interpretations of the concept of civilization in the scientific literature are analyzed, the conclusion is substantiated that it is necessary to combine the principles and mechanisms of interaction of the immutable logic of the general historical imperatives and the requirements of the domestic political tradition. The task of developing a socio-political theory of space, characteristics and features of the civilizational path of Russia is identified as urgent priorities.
MODERN SINO-AMERICAN INTERNATIONAL RELATIONS IN THE CONTEXT OF GLOBALIZATION
Authors:   K.G. Razumovsky Moscow State University of Tehnologies and Management (the First Cossack University), Moscow, Russian Federation, SMIRNOV ALEXANDER , ERMAKOV DMITRIY , KAZENKOV OLEG ,   Institute of Institute of the Far East of the Russian Academy of Sciences of the Russian Academy of Sciences,   “Polyus” Research Institute of M.F. Stelmakh
Published: September 2022

The nineties of the twentieth century led to changes in the global geopolitical strategy: the place of the Soviet Union, acting as the second world superpower, is gradually firmly occupied by the People’s Republic of China, which thirty years ago could count solely on the role of a regional leader. The development of international trade and economic relations between the United States and China clearly demonstrates in action the Hegelian “law of unity and struggle of opposites” - both countries, having different political systems and social structure, are forced to act as the main partners both in the world economy and in trade. The initiative in developing trade and economic relations with China is still in the hands of the United States, which, despite the availability of the latest technologies and outstanding economic achievements, still have to reckon with the requirements of its Chinese partner to establish equal partnership relations, bearing both material and reputational costs. The United States, throughout the history of US-Chinese relations, often uses provocations and unfriendly actions. The inconsistent position of the United States, primarily in support of Taiwan, makes it difficult to understand and establish trust between the countries.
Automation of preparation and tax administration reporting processes across countries: conceptual approaches
Authors:   SHEE “Kyiv National Economic University named after Vadym Hetman”, NIKOLENKO Larysa
Published: September 2022

Introduction. Preparation of Country-by-Country Reporting (CbCR) in accordance with the requirements of BEPS13, which is submitted by the parent company of an international group of companies to the jurisdiction by location, should be automatic and based on accounting and financial data. Companies need to prepare a consistent three-level reporting package that discloses information about the global distribution of income, economic activity of companies at the global level. In this regard, there is a need for IT tools to support the implementation of the initiative to comply with contractual obligations to exchange information. Problem Statement. The lack of a conceptual solution to automate the process of preparing the CbCR report, which will allow to prepare the report considering the characteristics of groups of companies and ensure the confidentiality of its use and tax administration, makes it impossible to obtain information quickly. Purpose. Highlighting the prospects for the introduction of the standard for automatic exchange of tax information in Ukraine and setting tasks for the development of a universal solution for automating the processes of reporting and tax administration in order to combat income tax evasion. Methods. General scientific and special methods are used: analysis, synthesis, grouping, description, comparison, system approach, modeling and generalization. Results.The basis for the preparation and analysis of the CbCR report for further automatic exchange, in accordance with the BEPS plan (step 13), is the automation of reporting, which is based on the construction of a unified accounting methodology, optimization of CbCR preparation methods, integration with the accounting systems of various members of the МGC and updating XML schemas with considering the requirements of different tax jurisdictions. It is the development of a comprehensive solution that includes analytical, methodological tools and has a reliable degree of protection to preserve the confidentiality of information, which will help to get an overall picture of the current tax situation of the MGC and the profit erosion risks. Conclusions. In order to achieve the set goal and objectives, conceptual approaches are presented for the development of a universal solution for automating the processes of reporting and tax administration with the aim of countering BEPS in the areas of implementation of OECD norms on the confidentiality of information that is the subject of international exchange.
In silico approach to identify microsatellite candidate biomarkers to differentiate the biovar of Corynebacterium pseudotuberculosis genomes
Authors: Pinheiro Kenny da Costa , Gois Bruna Verônica Azevedo , Nogueira Wylerson Guimarães , Araújo Fabrício Almeida , Queiroz Ana Lídia Cavalcante , Cardenas-Alegria Oscar , da Silva Artur Luiz da Costa , Júnior Antônio Márcio Gomes Martins , Ramos Rommel Thiago Jucá
Published: September 2022

Corynebacterium pseudotuberculosis is the causative bacterial agent of the zoonotic disease known as caseous lymphadenitis, and it presents several mechanisms of response to host defenses, including the presence of virulence factors (VFs). The genomes of these bacteria have several polymorphic markers known as microsatellites, or simple sequence repeats (SSRs), that can be used to characterize the genome, to study possible polymorphisms existing among strains, and to verify the effects of such polymorphic markers in coding regions and regions associated with VFs. In this study, several SSRs were identified within coding regions throughout the 54 genomes of this species, revealing possible polymorphisms associated with coding regions that could be used as strain-specific or serotype-specific identifiers of C. pseudotuberculosis. The similarities associated with SSRs amongst the different serum variants of C. pseudotuberculosis, biovars equi and ovis, were also evaluated, and it was possible to identify SSRs located in coding regions responsible for a VF enrolled in pathogenesis known to mediate bacterial adherence (SpaH-type pili virulence factor). Phylogenetic analyses revealed that strains sharing SSR patterns, including the possible polymorphisms identified in the same position of gene-coding regions, were displayed by strains with a common ancestor, corroborating with the Genome Tree Report of the NCBI. Statistical analysis showed that the microsatellite groups belonging to equi and ovis biovars have a significance of 0.006 (p-value) in similarity, thus indicating them as good biomarker candidates for C. pseudotuberculosis.
The potential therapeutic role of extracellular vesicles in osteoarthritis
Authors: Zhuang Yu , Jiang Shengjie , Yuan Changyong , Lin Kaili
Published: September 2022

Osteoarthritis (OA) is a worldwide and disabling disease, which cause severe pain and heavy socioeconomic burden. However, pharmacologic or surgical therapies cannot mitigate OA progression. Mesenchymal stem cells (MSCs) therapy has emerged as potential approach for OA treatment, while the immunogenicity and ethical audit of cell therapy are unavoidable. Compared with stem cell strategy, EVs induce less immunological rejection, and they are more stable for storage and in vivo application. MSC-EVs-based therapy possesses great potential in regulating inflammation and promoting cartilage matrix reconstruction in OA treatment. To enhance the therapeutic effect, delivery efficiency, tissue specificity and safety, EVs can be engineered via different modification strategies. Here, the application of MSC-EVs in OA treatment and the potential underlying mechanism were summarized. Moreover, EV modification strategies including indirect MSC modification and direct EV modification were reviewed.
Psychological stress creates an immune suppressive environment in the lung that increases susceptibility of aged mice to Mycobacterium tuberculosis infection
Authors: Lafuse William P. , Wu Qian , Kumar Naresh , Saljoughian Noushin , Sunkum Shrayes , Ahumada Omar Santiagonunez , Turner Joanne , Rajaram Murugesan V. S.
Published: September 2022

Age is a major risk factor for chronic infections, including tuberculosis (TB). Elderly TB patients also suffer from elevated levels of psychological stress. It is not clear how psychological stress impacts immune response toMycobacterium tuberculosis(M.tb).In this study, we used social disruption stress (SDR) to investigate effects of psychological stress in young and old mice. Unexpectedly, we found that SDR suppresses lung inflammation in old mice as evidenced by lower pro-inflammatory cytokine levels in bronchial lavage fluid and decreased cytokine mRNA expression by alveolar macrophages. To investigate effects of stress onM.tbinfection, mice were subjected to SDR and then infected withM.tb. As previously reported, old mice were better at controlling infection at 30 days than young mice. This control was transient as CFUs at 60 days were higher in old control mice compared to young mice. Consistently, SDR significantly increasedM.tbgrowth at 60 days in old mice compared to young mice. In addition, SDR in old mice resulted in accumulation of IL-10 mRNA and decreased IFN-γ mRNA at 60 days. Also, confocal microscopy of lung sections from old SDR mice showed increased number of CD4 T cells which express LAG3 and CD49b, markers of IL-10 secreting regulatory T cells. Further, we also demonstrated that CD4 T cells from old SDR mice express IL-10. Thus, we conclude that psychological stress in old mice prior to infection, increases differentiation of IL-10 secreting T cells, which over time results in loss of control of the infection.
Word-to-text integration components in second language (L2) reading comprehension
Authors: Alshehri Mona , Zhang Dongbo
Published: September 2022

Recognition of individual words serves as an initial basis for comprehension of a written text; yet there are complex word-to-text (WTT) integration processes underlying the comprehension. This study focused on two components of WTT integration, that is, syntactic parsing and semantic association, and assessed how syntactic and semantic network knowledge differentially predicted two types of text comprehension (literal vs. inferential) in second language readers. Participants were 229 adult learners of English language as a foreign language at a Saudi University. A battery of tasks was administrated to measure their reading comprehension, syntactic knowledge (grammatical error correction), and semantic network knowledge (semantic association), together with working memory and vocabulary knowledge/size. Multiple regression analyses showed that both syntactic and semantic network knowledge significantly predicted reading comprehension (disregarding the type of comprehension), controlling for working memory and vocabulary knowledge. Syntactic knowledge, as opposed to semantic network knowledge, was a significant, unique predictor of literal comprehension, whereas a converse pattern was found for inferential comprehension.
Factors associated with therapeutic response in acromegaly diagnosed in the elderly in Spain
Authors: Biagetti Betina , Iglesias Pedro , Villar-Taibo Rocío , Moure María-Dolores , Paja Miguel , Araujo-Castro Marta , Ares Jessica , Álvarez-Escola Cristina , Vicente Almudena , Álvarez Guivernau Èlia , Novoa-Testa Iria , Guerrero Perez Fernando , Cámara Rosa , Lecumberri Beatriz , García Gómez Carlos , Bernabéu Ignacio , Manjón Laura , Gaztambide Sonia , Cordido Fernando , Webb Susan M. , Menéndez-Torre Edelmiro Luis , Díez Juan J. , Simó Rafael , Puig-Domingo Manel
Published: September 2022

Context

Some reports suggest that acromegaly in elderly patients has a more benign clinical behavior and could have a better response to first-generation long-acting somatostatin receptor ligands (SRL). However, there is no specific therapeutic protocol for this special subgroup of patients.

Objective

This study aimed at identifying predictors of response to SRL in elderly patients.

Design

Multicentric retrospective nationwide study of patients diagnosed with acromegaly at or over the age of 65 years.

Results

One-hundred and eighteen patients (34 men, 84 women, mean age at diagnosis 71.7 ± 5.4 years old) were included. Basal insulin-like growth factor type 1 (IGF-1) above the upper limit of normal (ULN) and growth hormone (GH) levels (mean ± SD) were 2.7 ± 1.4 and 11.0 ± 11.9 ng/ml, respectively. The mean maximal tumor diameter was 12.3 ± 6.4 mm, and up to 68.6% were macroadenoma. Seventy-two out of 118 patients (61.0%) underwent surgery as primary treatment. One-third of patients required first-line medical treatment due to a rejection of surgical treatment or non-suitability because of high surgical risk. After first-line surgery, 45/72 (63.9%) were in disease remission, and 16/34 (46.7%) of those treated with SRL had controlled disease. Patients with basal GH at diagnosis ≤6 ng/ml had lower IGF-1 levels and had smaller tumors, and more patients in this group reached control with SRL (72.7% vs. 33.3%; p < 0.04) [OR: 21.3, IC: 95% (2.4–91.1)], while male patients had a worse response [OR: 0.09, IC 95% (0.01–0.75)]. The predictive model curve obtained for SRL response showed an AUC of 0.82 CI (0.71–0.94).

Conclusions

The most frequent phenotype in newly diagnosed acromegaly in the elderly includes small adenomas and moderately high IGF-1 levels. GH at diagnosis ≤6 ng/ml and female gender, but not age per se, were associated with a greater chance of response to SRL.
Spatial differentiation and influencing factors of green finance development level in China
Authors: Zhou Yuanxiang , Liu Huayan , Wang Jingxuan , Yang Hongrui , Liu Ziyun , Peng Gang
Published: September 2022

The entropy method measures the development level of green finance in 30 provinces and cities in China, and the spatial analysis method is used to describe the difference. Spatial pattern of the development level of green finance in China and empirically analyze the influencing factors of the development level of green finance. The results of the spatial econometric model show that the absolute difference in the development level of China’s green finance shows an overall increasing trend, and the relative difference shows a downward trend year by year. The level of financial development and environmental governance promote the development of green finance, while the level of economic growth and energy consumption inhibit the development of green finance. On this basis, we put forward countermeasures and suggestions from strengthening government functions and adjusting industrial structure according to economic growth, so as to promote the development of green finance in China.
Focus on long non-coding RNA MALAT1: Insights into acute and chronic lung diseases
Authors: Lai Xingning , Zhong Jie , Zhang Aihua , Zhang Boyi , Zhu Tao , Liao Ren
Published: September 2022

Acute lung injury (ALI) is a pulmonary illness with a high burden of morbidity and mortality around the world. Chronic lung diseases also represent life-threatening situations. Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) is a type of long non-coding RNA (lncRNA) and is highly abundant in lung tissues. MALAT1 can function as a competitive endogenous RNA (ceRNA) to impair the microRNA (miRNA) inhibition on targeted messenger RNAs (mRNAs). In this review, we summarized that MALAT1 mainly participates in pulmonary cell biology and lung inflammation. Therefore, MALAT1 can positively or negatively regulate ALI and chronic lung diseases (e.g., chronic obstructive pulmonary disease (COPD), bronchopulmonary dysplasia (BPD), pulmonary fibrosis, asthma, and pulmonary hypertension (PH)). Besides, we also found a MALAT1-miRNA-mRNA ceRNA regulatory network in acute and chronic lung diseases. Through this review, we hope to cast light on the regulatory mechanisms of MALAT1 in ALI and chronic lung disease and provide a promising approach for lung disease treatment.
Diversity of the gut, vaginal and oral microbiome among pregnant women in South Africa with and without pre-eclampsia
Authors: Geldenhuys Janri , Redelinghuys Mathys J. , Lombaard Hendrik A. , Ehlers Marthie M. , Cowan Don , Kock Marleen M.
Published: September 2022

Background

Changes in microbial communities are a known characteristic of various inflammatory diseases and have been linked to adverse pregnancy outcomes, such as preterm birth. However, there is a paucity of information regarding the taxonomic composition and/or diversity of microbial communities in pre-eclampsia. The aim of this study was to determine the diversity of the gut, vaginal and oral microbiome in a cohort of South African pregnant women with and without pre-eclampsia. The diversity of the gut, vaginal and oral microbiome was determined by targeted next generation sequencing (NGS) of the V3 and V4 region of the 16S rRNA gene on the Illumina MiSeq platform.

Results

In this study population, pre-eclampsia was associated with a significantly higher alpha diversity (P = 0.0472; indicated by the Shannon index) in the vaginal microbiome accompanied with a significant reduction in Lactobacillus spp. (P = 0.0275), compared to normotensive pregnant women. Lactobacillus iners was identified as the predominant species of the vaginal microbiome in both cohorts. High inter-individual variation in alpha diversity was observed in the gut and oral microbiome in both cohorts. Although differences in the relative abundance of bacteria at all phylogenetic levels were observed, overall microbial composition of the gut, oral and vaginal microbiome was not significantly different in the pre-eclampsia cohort compared to the normotensive cohort.

Conclusion

Collectively, a reduction of Lactobacillus spp., and predominance of L. iners in pregnant women with pre-eclampsia could suggest an unstable vaginal microbiome that might predispose pregnant women to develop pre-eclampsia. The lack of significant structural changes in the gut, oral and vaginal microbiome does not suggest that the characterized communities play a role in pre-eclampsia, but could indicate a characteristic unique to the study population. The current study provided novel information on the diversity of the gut, oral and vaginal microbiome among pregnant women in South Africa with and without pre-eclampsia. The current study provides a baseline for further investigations on the potential role of microbial communities in pre-eclampsia.
Transcriptome profiling combined with network analysis deepens the understanding of immune response mechanisms in blood of pacific oyster Crassostrea gigas infected by Vibrio alginolyticus
Authors: Zhang Enshuo , Dong Luyao , Bao Xiaokai , Yang Xinyu , Li Yuxin , Feng Yanwei , Yang Jianmin , Li Zan , Wang Weijun
Published: September 2022

Mass mortalities of oysters during the summer have been reported for years. Among the biological factors causing oyster death in summer, Vibrio infection is considered to be the most important factor. Vibrio alginolyticus is one of the main pathogens causing oyster diseases. V. alginolyticus can cause disease and death of fish, shrimp, shellfish, and other aquatic products. Human infection with V. alginolyticus can also cause skin infections, external ear canal infections, and other diseases. Hemocytes in the blood of Crassostrea gigas resemble vertebrate macrophages and play an important role in the immune regulation of organisms. Therefore, it is of great significance to analyze the blood transcriptome to study the anti-pathogen mechanism of the C. gigas. In our study, we performed a transcriptome profile of gene expression on C. gigas infected with V. alginolyticus at 12 and 48 hours, and identified 2,494 and 1,165 differentially expressed genes (DEGs) at two sampling time points. Then, the functions of DEGs were identified using GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) functional enrichment analysis, revealing that there were multiple significantly enriched GO terms and KEGG signaling pathways related to immunity. And a protein-protein interaction (PPI) network was construct using DEGs enriched in significant enriched immune-related KEGG signaling pathways. At last, we screened and validated 12 key genes with multiple protein interaction numbers or involved in more KEGG signaling pathways, and verified their expression changes by quantitative RT-PCR (qRT-PCR). For the first time, we studied the immune defense mechanism of C. gigas blood based on protein-protein interaction network, which explains how C. gigas lacking specific immunity survive in a pathogen-rich environment. This research provides reference for solving the problem of high mortality of C. gigas and other mollusks in summer, and provides reference for the future production of some disease-resistant C. gigas.
A real-time passive acoustic monitoring system to detect Yangtze finless porpoise clicks in Ganjiang River, China
Authors: Li Weilun , Qiu Jiansong , Lei Peiyu , Chen Xiaohong , Fan Fei , Deng Xiaojun , Dai Yingen , Deng Yanqing , Wang Kexiong , Mei Zhigang
Published: September 2022

Phocoenidae live in fresh, coastal waters where they often share a significant portion of their habitat with humans. As a result, local activities (e.g., coastal fisheries and shipments) cause underwater noise pollution and threaten their ecosystem. To better conserve the habitat of porpoises, we aimed to study their activities in these waters by recording their echolocation clicks using a passive acoustic monitoring (PAM) system. However, because the off-line PAM instruments were often used in the past that need to be periodically deployed and recovered, data acquisition is typically obtained and analyzed in batches, rather than in real-time. A real-time PAM detection system would help minimize the impact of underwater noise on approaching porpoises. Furthermore, issues of bad quality data–with gaps due to loss or damage of the off-line PAM instruments–could be avoided with a real-time detection system. Therefore, in this study, we developed the Real-time Porpoise Click Detector-II (RPCD-II), equipped with a digital hydrophone, main memory (2 TB storage), a central processing unit, and a wireless transmission module. We deployed the RPCD-II under a docked fishing vessel at the Ganjiang River in Yangzi Zhou Town Fisheries Village, Nanchang City (8–9 December 2021), where it recorded signals of Yangtze finless porpoise and produced a real-time report. To validate the results of RPCD-II, another underwater sound recorder, the SoundTrap 300HF (ST), was also set up (as a control device) under the docked fishing vessel. Both devices recorded consistent results of 9330 clicks, further demonstrating RPCD-II’s ability for the real-time detection of Yangtze finless porpoise in the field.
Emerging prediction methods for early diagnosis of necrotizing enterocolitis
Authors: Wu Siyuan , Di Sijia , Liu Tianjing , Shi Yongyan
Published: September 2022

Necrotizing enterocolitis (NEC) is a life-threatening disease of the digestive system that occurs in the neonatal period. NEC is difficult to diagnose early and the prognosis is poor. Previous studies have reported that abnormalities can be detected before the presentation of clinical symptoms. Based on an analysis of literature related to the early prediction of NEC, we provide a detailed review on the early prediction and diagnosis methods of NEC, including ultrasound, near-infrared spectroscopy, biomarkers, and intestinal microbiota. This review aimed to provide a reference for further research and clinical practice.
Effects of g-C3N4 on bacterial community and tetracycline resistance genes in two typical sediments in tetracycline pollution remediation
Authors: Hu Xuemei , Chen Xiaoyong , Tang Yao , Xu Zhenggang , Zeng Yelin , Wang Yonghong , Zhao Yunlin , Wu Yaohui , Wang Guangjun
Published: September 2022

Photocatalysis, as a novel technique, has been widely used for antibiotic pollution remediation in wastewater. In the processes of degradation and removal of antibiotics, the impact of photocatalysts on microenvironment is very important but remains poorly understood. In the present study, the effect of typical photocatalyst g-C3N4 (Graphitic carbon nitride) on microbial community was investigated in two sediment types (riverbed sediment and pig-farm sediment) polluted by tetracycline (TC) in central southern China. The riverbed sediment and pig farm sediment samples were respectively exposed to g-C3N4 (25, 75, 125 mg⋅kg–1) and TC (60, 120, 180 mg⋅L–1) treatments alone or combination for 30 days, respectively. The bacterial community and antibiotic resistance genes (ARGs) of the treated sediments were analyzed by Illumina sequencing and metagenomic sequencing. Studies had shown that: TC, g-C3N4, and TC/g-C3N4 have significant effects on the changes of microbial communities and components in riverbed sediment, but they do not exist in pig farm sediment. The most alterations of microbial taxa were Acidobacteriota, Actinobacteriota, and Desulfobacterota in riverbed sediment, and Elusimicrobiota in the pig farm sediment under various treatments. Through network analysis, it was found that the distribution of microorganisms in the pig farm sediment is more complex and more stable. The addition of g-C3N4 reduced the absolute abundance of ARGs in the two examined sediments, but not significantly changed their relative abundance of ARGs. The g-C3N4 application was beneficial to the removal of TC residues and to the prevention of the generation and transmission of ARGs in sediments. Our results suggested that g-C3N4 was a suitable photocatalyst with excellent application prospect for the removal of TC residues and the control of ARGs in environment.
Antarctic krill oil exhibited synergistic effects with nobiletin and theanine in ameliorating memory and cognitive deficiency in SAMP8 mice: Applying the perspective of the sea–land combination to retard brain aging
Authors: Wang Cheng-Cheng , Kong Jing-Ya , Li Xiao-Yue , Yang Jin-Yue , Xue Chang-Hu , Yanagita Teruyoshi , Wang Yu-Ming
Published: September 2022

The complex pathogenesis of Alzheimer's disease (AD) leads to a limited therapeutic effect; therefore, the combination of multiple bioactive ingredients may be more effective in improving AD due to synergistic effects. Based on the perspective of the sea–land combination, the effects of sea-derived Antarctic krill oil (AKO) combined with land-derived nobiletin (Nob) and L-theanine (The) on memory loss and cognitive deficiency were studied in senescence-accelerated prone 8 mice (SAMP8). The results demonstrated that AKO combined with The significantly increased the number of platform crossings in the Morris water maze test by 1.6-fold, and AKO combined with Nob significantly increased the preference index in a novel object recognition test. AKO exhibited synergistic effects with Nob and The in ameliorating recognition memory and spatial memory deficiency in SAMP8 mice, respectively. Further research of the mechanism indicated that AKO exhibited synergistic effects with Nob in suppressing β-amyloid (Aβ) aggregation, neurofibrillary tangles, and apoptosis and neuroinflammation, while the synergistic effects of AKO and The involved in synaptic plasticity and anti-neuroinflammation, which revealed that the combination was complex, not a mechanical addition. These findings revealed that the sea–land combination may be an effective strategy to treat and alleviate AD.
Epidemiology of intracerebral hemorrhage: A systematic review and meta-analysis
Authors: Wang Sai , Zou Xue-Lun , Wu Lian-Xu , Zhou Hui-Fang , Xiao Linxiao , Yao Tianxing , Zhang Yupeng , Ma Junyi , Zeng Yi , Zhang Le
Published: September 2022

Background

Intracerebral hemorrhage (ICH) is associated with high mortality and disability rates. This study aimed to investigate the relationship between sex, age, study year, risk factors, bleeding site, median year of study, and the incidence of ICH.

Method

Literature on the incidence of ICH published on 1 January 1980 and 1 January 2020, was systematically retrieved from PubMed and Embase databases. The random-effects model and subgroup analysis were used to explore the relationship between the incidence of ICH and different ages, sex, bleeding sites, and risk factors.

Results

We summarized the epidemiological changes in ICH in the past 40 years according to 52 studies and found that the total incidence of ICH is 29.9 per 100,000 person-years (95% CI: 26.5–33.3), which has not decreased worldwide. The incidence of ICH in the Asian population is much higher than in other continents. In addition, the incidence of ICH increases with age and differs at the 85-year-old boundary. Men are more likely to develop ICH than women, and the basal ganglia region is the most common area for ICH. Of the 10 risk factors examined in this study, those with hypertension had the highest incidence of ICH, followed by those with excessive alcohol consumption and heart disease.

Conclusion

The prevention and treatment of ICH still need to be improved continuously according to age, sex, risk factors, and other factors, and targeted and normative strategies should be gradually developed in the future.
Research hotspots and frontiers about role of visual perception in stroke: A bibliometric study
Authors: Zhang Nannan , Li Chong , Chen Jianmin , Liu Xiahua , Wang Zhiyong , Ni Jun
Published: September 2022

Background

Visual perception is a dynamic process of perceiving the environment through sensory input and transforming sensory input into meaningful concepts related to environmental visual knowledge. Many studies focusing on the role of visual perception after stroke have been published in various journals. However, a bibliometric analysis in the domain of visual perception after stroke is still lacking. This study aimed to deliver a visual analysis to analyze the global trends in research on the role of visual perception after stroke in the last 10 years.

Methods

The literature was derived from the Web of Science core collection database from 2012 to 2021. The collected material was limited to English articles and reviews. CiteSpace and Microsoft Excel were used for bibliographic analysis.

Results

A total of 298 articles were included in the analysis. The annual number of publications increased from 23 to 42 in the last decade. Rehabilitation was the main research hotspot (n = 85). Journal of Physical Therapy Science published the largest number of papers (n = 14). The most influential author, institution, and country were Rowe FJ (n = 17), League of European Research Universities (n = 45), and England (n = 54), respectively. The keywords with the longest burst period are field defect, hemineglect, disorder, and quality of life.

Conclusion

This study analyzes the papers on the role of visual perception after stroke in the past 10 years and provides a new perspective for research in this field. At present, the number of articles in this field is not large and the cooperation network is not close enough. In the future, it is necessary to strengthen the cooperation among various countries, institutions, and authors. In addition, large samples and randomized controlled trials are needed to identify the potential treatments and pathophysiology for visual perceptual impairment after stroke.
Ras GTPase-activating proteins control neuronal circuit development in barrel cortex layer 4
Authors: Rao Madhura S. , Mizuno Hiromi , Iwasato Takuji , Mizuno Hidenobu
Published: September 2022

The cerebral cortex comprises a complex and exquisite network of neuronal circuits that is formed during development. To explore the molecular mechanisms involved in cortical circuit formation, the tactile somatosensory pathway that connects the whiskers and cortex of rodents is a useful model. Here, we analyzed the roles of Ras GTPase-activating proteins (RasGAPs) in the circuit formation in the somatosensory cortex layer 4 (L4). We suppressed the function of RasGAPs in L4 neurons using Supernova RNAi, a plasmid vector-based sparse cell gene knockdown (KD) system. The results showed disrupted dendritic pattern formation of L4 spiny stellate neurons on the barrel edge by RasGAP KD. Furthermore, the number of presynaptic boutons on L4 neurons was reduced by RasGAP KD. These results demonstrate the essential roles of RasGAPs in circuit formation in the cerebral cortex and imply that developmental changes in dendrites and synapses in RasGAP KD neurons may be related to cognitive disabilities in RasGAP-deficient individuals, such as patients with neurofibromatosis type 1.
Effects of robot-assisted task-oriented upper limb motor training on neuroplasticity in stroke patients with different degrees of motor dysfunction: A neuroimaging motor evaluation index
Authors: Xie Hui , Li Xin , Huang Wenhao , Yin Jiahui , Luo Cailing , Li Zengyong , Dou Zulin
Published: September 2022

Introduction

Although robot-assisted task-oriented upper limb (UL) motor training had been shown to be effective for UL functional rehabilitation after stroke, it did not improve UL motor function more than conventional therapy. Due to the lack of evaluation of neurological indicators, it was difficult to confirm the robot treatment parameters and clinical efficacy in a timely manner. This study aimed to explore the changes in neuroplasticity induced by robot-assisted task-oriented UL motor training in different degrees of dysfunction patients and extract neurological evaluation indicators to provide the robot with additional parameter information.

Materials and methods

A total of 33 adult patients with hemiplegic motor impairment after stroke were recruited as participants in this study, and a manual muscle test divided patients into muscle strength 0–1 level (severe group, n = 10), 2–3 level (moderate group, n = 14), and 4 or above level (mild group, n = 9). Tissue concentration of oxyhemoglobin and deoxyhemoglobin oscillations in the bilateral prefrontal cortex, dorsolateral prefrontal cortex (DLPFC), superior frontal cortex (SFC), premotor cortex, primary motor cortex (M1), primary somatosensory cortex (S1), and occipital cortex were measured by functional near-infrared spectroscopy (fNIRS) in resting and motor training state. The phase information of a 0.01 −0.08 Hz signal was identified by the wavelet transform method. The wavelet amplitude, lateralization index, and wavelet phase coherence (WPCO) were calculated to describe the frequency-specific cortical changes.

Results

Compared with the resting state, significant increased cortical activation was observed in ipsilesional SFC in the mild group and bilateral SFC in the moderate group during UL motor training. Patients in the mild group demonstrated significantly decreased lateralization of activation in motor training than resting state. Moreover, the WPCO value of motor training between contralesional DLPFC and ipsilesional SFC, bilateral SFC, contralesional, S1, and ipsilesional M1 showed a significant decrease compared with the resting state in the mild group.

Conclusion

Robot-assisted task-oriented UL motor training could modify the neuroplasticity of SFC and contribute to control movements and continuous learning motor regularity for patients. fNIRS could provide a variety of real-time sensitive neural evaluation indicators for the robot, which was beneficial to formulating more reasonable and effective personalized prescriptions during motor training.
Deep learning-based self-induced emotion recognition using EEG
Authors: Ji Yerim , Dong Suh-Yeon
Published: September 2022

Emotion recognition from electroencephalogram (EEG) signals requires accurate and efficient signal processing and feature extraction. Deep learning technology has enabled the automatic extraction of raw EEG signal features that contribute to classifying emotions more accurately. Despite such advances, classification of emotions from EEG signals, especially recorded during recalling specific memories or imagining emotional situations has not yet been investigated. In addition, high-density EEG signal classification using deep neural networks faces challenges, such as high computational complexity, redundant channels, and low accuracy. To address these problems, we evaluate the effects of using a simple channel selection method for classifying self-induced emotions based on deep learning. The experiments demonstrate that selecting key channels based on signal statistics can reduce the computational complexity by 89% without decreasing the classification accuracy. The channel selection method with the highest accuracy was the kurtosis-based method, which achieved accuracies of 79.03% and 79.36% for the valence and arousal scales, respectively. The experimental results show that the proposed framework outperforms conventional methods, even though it uses fewer channels. Our proposed method can be beneficial for the effective use of EEG signals in practical applications.
Identification and validation of a novel necroptosis-related prognostic signature in cervical squamous cell carcinoma and endocervical adenocarcinoma
Authors: Zhang Weiyu , Cao Wujun , Tong Zhuting , Jin Qinqin , Jiang Xiya , Yang Yinting , Yao Hui , Chen Guo , Gao Wei , Zhu Yuting , Zhou Shuguang
Published: September 2022

Background

The purpose of this study was to investigate the prognostic signature of necroptosis-related lncRNAs (NRLs) and explore their association with immune-related functions and sensitivity of the therapeutic drug in cervical squamous cell carcinoma and endocervical adenocarcinoma (CESC).

Methods

UCSC Xena provided lncRNA sequencing and clinical data about CESC, and a necroptosis gene list was obtained from the KEGG database. NRLs were selected by structuring a co-expression network of lncRNAs and necroptosis-related genes. To further screen lncRNAs, we used the univariate Cox regression method, Lasso regression, and multivariate Cox regression. Afterward, an NRL signature was established. We used the xCell algorithm and single-sample gene set enrichment analysis (ssGSEA) to clarify the pertinence between immune infiltration and NRL expressions in CESC patients and explored the relationship between the target lncRNAs and immune-related genes. By leveraging the GDSC database, the therapy-sensitive response of the prognostic signature was forecasted and an experimental validation was performed. We performed GSEA with the aim of recognizing the potential pathway related to the individual prognostic signature.

Results

The two prognostic NRLs (AC009095.1 and AC005332.4) showed significant diversity and constituted the NRL signature. On the grounds of our signature, risk score was an independent element which was bound up with patient outcome (HR = 4.97 CI: 1.87–13.2, P = 0.001). The CESC patients were classified by the median risk score. Immune infiltration analysis revealed significant increases in CD4 + Tcm, eosinophils, epithelial cells, fibroblasts, NKT, plasma cells, platelets, and smooth muscle in the high-risk group (P< 0.05). Target lncRNAs also showed some correlation with NRGs. The estimated IC50 values of bicalutamide, CHIR.99021, and imatinib were lower in the high-risk group. Through the subsequent experimental validation, both AC009095.1 and AC005332.4 were significantly more highly expressed in SiHa than in Hela. AC009095.1 was expressed more highly in SiHa than in HUCEC, but the expression of AC005332.4 was reversed.

Conclusions

This study elucidated that NRLs, as a novel signature, were indispensable factors which can significantly influence the prognosis of patients with CESC and could provide novel clinical evidence to serve as a potential molecular biomarker for future therapeutic targets.
Microenvironment immune response induced by tumor ferroptosis—the application of nanomedicine
Authors: Yun Tian , Liu Zhenzhu , Wang Jianbo , Wang Rui , Zhu Liang , Zhu Zheng , Wang Xuejian
Published: September 2022

Ferroptosis is a non-apoptotic regulatory form of cell death that has sparked significant interest and research in cancer treatment and certain small chemical inducers have been used in the clinic. These inducers’s weak water solubility, poor targeting, rapid metabolism; and other undesirable characteristics; however, for therapeutic approaches that combine immunotherapy and ferroptosis, challenges such as medication delivery, the complexity of the tumor microenvironment, and immunosuppression remain. The targeted, low toxicity, and efficient distribution benefits of nanotechnology have considerably enhanced the therapeutic efficacy of combining immunotherapy with ferroptosis. This paper describes the distinct mechanism of ferroptosis in tumor therapy and immunotherapy, as well as the application and benefits of nanotechnology in the combination of tumor immunotherapy and ferroptosis.

Systematic review registration

http://clinicaltrials.gov/, NCT00941070.
TRAF3IP3 promotes glioma progression through the ERK signaling pathway
Authors: Lin Qi , Chen Zhen , Shen Zhao-Li , Xue Fei , Qin Jia-Jun , Kang Xi-Peng , Chen Zhong-Rong , Xia Zhong -Yuan , Gao Liang , Chen Xian-Zhen
Published: September 2022

TRAF3IP3 was reportedly associated with poor prognosis in patients with melanoma; however, its role in glioma is unknown. We aimed to demonstrate the relationship between TRAF3IP3 and glioma and to investigate the potential role of TRAF3IP3 in glioma. Datasets were collected from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. We used the Wilcoxon rank-sum test to compared TRAF3IP3 expression in normal and glioma tissues. Kaplan–Meier analysis was performed to evaluate the correlation between TRAF3IP3 and patient survival rate. Gene set enrichment analysis (GSEA) was used to annotate the biological function of TRAF3IP3 in glioma. We also examined the effects of TRAF3IP3 on glioma progression, including characteristics such as cell proliferation, migration, and invasion, using cell proliferation, wound healing, and Transwell assays, respectively, paired with in vitro glioma cell lines and in vivo mouse xenograft models to determine the molecular mechanisms underlying these effects. High TRAF3IP3 expression in glioma tissues was associated with patients with neoplasm cancer tissue source site, and poorer overall survival (OS) (p = 0.03), which was validated using TCGA. GSEA revealed the enrichment of neuroactive ligand–receptor interactions, the olfactory pathway, proteasome pathway, cytokine–cytokine receptor interactions, and calcium signaling pathway in the TRAF3IP3 high-expression phenotype. TRAF3IP3 knockdown markedly suppressed the proliferation, migration, and invasion abilities of U251 glioma cells, whereas TRAF3IP3 overexpression notably promoted the progression of U118 cell tumors. Mechanistic studies revealed that TRAF3IP3 upregulated p-ERK expression in glioma cells. Notably, the ERK signaling pathway inhibitor U0126 drastically attenuated the effects of TRAF3IP3 on p-ERK and markedly blocked its tumor-promoting activity. TRAF3IP3 overexpression also promoted in vivo tumor growth in a nude mouse xenograft model. Collectively, TRAF3IP3 stimulates glioma cell proliferation, migration, and invasion, at least partly by activating the ERK signaling pathway. We hypothesize that TRAF3IP3 may participate in glioma development via the ERK signaling pathway and that elevated TRAF3IP3 expression may serve as a potential biomarker for glioma prognosis.
Promising clinical outcome after body gamma knife radiotherapy for mediastinal follicular dendritic cell sarcoma with thoracic spine invasion and iliac metastasis: A case report and literature review
Authors: Hu Annan , Chen Ting , Dong Jian
Published: September 2022

Background

Follicular dendritic cell sarcoma (FDCS) is a rare type of intermediate grade tumor. Mediastinal FDCS with spinal invasion has not been well described. The treatment options include surgical resection and radiation therapy. The body gamma knife is a stereotactic body radiotherapy (SBRT) technology that is widely used in China. The pathological evaluation of a bone lesion after a body gamma knife procedure has not been reported. Here, we report a case of a patient with FDCS with thoracic spine invasion and iliac metastasis treated with surgery and body gamma knife.

Case summary

A 36-year-old male patient was hospitalized at Zhongshan Hospital, Fudan University, due to a gradually aggravated pain on the lateral side of the left scapula for 6 months. Imaging examination showed neoplastic lesions on the left side of C7-T2 invading the vertebral body of T1, T2, and caput costae of the second rib and suspected metastasis in the left ilium. FDCS was diagnosed after performing a computed tomography (CT)-guided core needle biopsy, and the thoracic lesion was surgically resected. The body gamma knife was used as an adjuvant radiotherapy for the thoracic lesion and a primary therapy for the left ilium lesion. Iliac bone lesion resection was performed at Zhongshan Hospital, Fudan University, 10 weeks after RT. Compared with the biopsy report, the body gamma knife treatment resulted in a pathological complete response (PCR). The magnetic resonance imaging (MRI) examinations showed stable disease of the thoracic lesion after body gamma knife radiosurgery.

Conclusion

This case report describes the treatment of mediastinal FDCS with thoracic spinal invasion and iliac metastasis. The promising outcome suggests that separation surgery is an effective treatment option for mediastinal FDCS with spinal column invasion. It also demonstrates the application prospects of the body gamma knife treatment in malignant lesions of the axial bones.
Treatment-associated remodeling of the pancreatic cancer endothelium at single-cell resolution
Authors: Shiau Carina , Su Jennifer , Guo Jimmy A. , Hong Theodore S. , Wo Jennifer Y. , Jagadeesh Karthik A. , Hwang William L.
Published: September 2022

Pancreatic ductal adenocarcinoma (PDAC) is one of the most treatment refractory and lethal malignancies. The diversity of endothelial cell (EC) lineages in the tumor microenvironment (TME) impacts the efficacy of antineoplastic therapies, which in turn remodel EC states and distributions. Here, we present a single-cell resolution framework of diverse EC lineages in the PDAC TME in the context of neoadjuvant chemotherapy, radiotherapy, and losartan. We analyzed a custom single-nucleus RNA-seq dataset derived from 37 primary PDAC specimens (18 untreated, 14 neoadjuvant FOLFIRINOX + chemoradiotherapy, 5 neoadjuvant FOLFIRINOX + chemoradiotherapy + losartan). A single-nucleus transcriptome analysis of 15,185 EC profiles revealed two state programs (ribosomal, cycling), four lineage programs (capillary, arterial, venous, lymphatic), and one program that did not overlap significantly with prior signatures but was enriched in pathways involved in vasculogenesis, stem-like state, response to wounding and hypoxia, and endothelial-to-mesenchymal transition (reactive EndMT). A bulk transcriptome analysis of two independent cohorts (n = 269 patients) revealed that the lymphatic and reactive EndMT lineage programs were significantly associated with poor clinical outcomes. While losartan and proton therapy were associated with reduced lymphatic ECs, these therapies also correlated with an increase in reactive EndMT. Thus, the development and inclusion of EndMT-inhibiting drugs (e.g., nintedanib) to a neoadjuvant chemoradiotherapy regimen featuring losartan and/or proton therapy may be most effective in depleting both lymphatic and reactive EndMT populations and potentially improving patient outcomes.