bluuebunny/crossref_metadata_2025 huggingface dataset:
Ochratoxin A and Kidney Oxidative Stress: The Role of Nutraceuticals in Veterinary Medicine—A Review
Authors: Longobardi Consiglia , Ferrara Gianmarco , Andretta Emanuela , Montagnaro Serena , Damiano Sara , Ciarcia Roberto
Published: June 2022

The problem of residues of toxic contaminants in food products has assumed considerable importance in terms of food safety. Naturally occurring contaminants, such as mycotoxins, are monitored routinely in the agricultural and food industries. Unfortunately, the consequences of the presence of mycotoxins in foodstuffs are evident in livestock farms, where both subacute and chronic effects on animal health are observed and could have non-negligible effects on human health. Ochratoxin A (OTA) is a common mycotoxin that contaminates food and feeds. Due to its thermal stability, the eradication of OTA from the food chain is very difficult. Consequently, humans and animals are frequently exposed to OTA in daily life. In this review article, we will devote time to highlighting the redox-based nephrotoxicity that occurs during OTA intoxication. In the past few decades, the literature has improved on the main molecules and enzymes involved in the redox signaling pathway as well as on some new antioxidant compounds as therapeutic strategies to counteract oxidative stress. The knowledge shown in this work will address the use of nutraceutical substances as dietary supplements, which would in turn improve the prophylactic and pharmacological treatment of redox-associated kidney diseases during OTA exposure, and will attempt to promote animal feed supplementation.
The Dual Roles of Protein-Bound Solutes as Toxins and Signaling Molecules in Uremia
Authors: Masereeuw Rosalinde
Published: June 2022

In patients with severe kidney disease, renal clearance is compromised, resulting in the accumulation of a plethora of endogenous waste molecules that cannot be removed by current dialysis techniques, the most often applied treatment. These uremic retention solutes, also named uremic toxins, are a heterogeneous group of organic compounds of which many are too large to be filtered and/or are protein-bound. Their renal excretion depends largely on renal tubular secretion, by which the binding is shifted towards the free fraction that can be eliminated. To facilitate this process, kidney proximal tubule cells are equipped with a range of transport proteins that cooperate in cellular uptake and urinary excretion. In recent years, innovations in dialysis techniques to advance uremic toxin removal, as well as treatments with drugs and/or dietary supplements that limit uremic toxin production, have provided some clinical improvements or are still in progress. This review gives an overview of these developments. Furthermore, the role protein-bound uremic toxins play in inter-organ communication, in particular between the gut (the side where toxins are produced) and the kidney (the side of their removal), is discussed.
Incidence and Risk Factors of Bilateral Herpetic Keratitis: 2022 Update
Authors: Chaloulis Stergios K , Mousteris Georgios , Tsaousis Konstantinos T
Published: June 2022

Simultaneously occurring bilateral herpetic keratitis is a rare clinical manifestation of ocular herpes. Immunocompromised patients are more susceptible to develop bilateral disease. The purpose of this study is to review recent literature on the topic and to highlight latest updates, regarding risk factors and possible correlations to the disease, as well as new therapeutic options.
Enhancing Tuberculosis Case-Finding: A Case of Quality Improvement Initiative in Tanzania
Authors: Wandwalo Eliud , Kamara Deus V. , Yassin Mohammed A. , Morrison Linden , Nwaneri Nnamdi B. , Asiimwe Sarah , Matiku Sode , Kisonga Riziki , Tarimo Allan
Published: June 2022

Background: Tanzania is 1 of the 30 high TB burden countries and 1 of the 13 countries in which 75% of people with TB are unaccounted for and that is prioritized for the Global Fund Catalytic investment and Strategic Initiative support. Tanzania decided to strengthen its National TB Programme to find these people with TB who are unaccounted for by identifying evidence-driven innovations to deliver high-quality services and to improve the efficiency of TB case-finding. A quality improvement (QI) initiative was implemented by the National Tuberculosis and Leprosy Programme to enhance TB case-finding. The initiative involved identifying gaps in the quality of services, introducing new tools, improving the work capacity of health care workers through training and mentorship sessions, strengthening laboratory and referral services, and implementing mandatory TB screening of all patients attending health facilities. We aimed to assess the effectiveness of QI initiative to enhance TB case-findings at the health facility level. Method: A cross-sectional design, and intervention and control facilities randomly selected for an evaluation of the QI initiative were used. Twenty facilities from the Dodoma region across all health care system levels (dispensaries, health centres, and hospitals) were involved in this evaluation. The facilities were randomly divided into either the intervention or control groups at a 1:1 ratio (10 intervention and 10 control facilities). Data routinely collected from program registers from January 2016 to June 2017 were used. Result: The evaluation registered a 52% increase in TB case notification in Q1 of 2017 compared with in Q1 of 2016 and, similarly, a 52% increase in Q2 of 2017 compared with in Q2 of 2016, with 9 out of 10 intervention sites reporting increases in their quarterly TB case notifications. There were no positive changes in the ‘control facilities’ where routine services were provided, with half of the facilities showing a decrease in TB case notification from baseline. Conclusion: This QI initiative has the potential to support a long-term comprehensive approach to ending TB and to improve the quality of the foundations of the health care system. This initiative sets a reliable pace for health facilities to efficiently respond to and manage TB case-finding interventions put into action. Tanzania’s experience with implementing QI interventions could serve as a model for improving TB case notifications in other settings.
Lorentz Symmetry Violation of Cosmic Photons
Authors: He Ping , Ma Bo-Qiang
Published: June 2022

As a basic symmetry of space-time, Lorentz symmetry has played important roles in various fields of physics, and it is a glamorous question whether Lorentz symmetry breaks. Since Einstein proposed special relativity, Lorentz symmetry has withstood very strict tests, but there are still motivations for Lorentz symmetry violation (LV) research from both theoretical consideration and experimental feasibility, that attract physicists to work on LV theories, phenomena and experimental tests with enthusiasm. There are many theoretical models including LV effects, and different theoretical models predict different LV phenomena, from which we can verify or constrain LV effects. Here, we introduce three types of LV theories: quantum gravity theory, space-time structure theory and effective field theory with extra-terms. Limited by the energy of particles, the experimental tests of LV are very difficult; however, due to the high energy and long propagation distance, high-energy particles from astronomical sources can be used for LV phenomenological researches. Especially with cosmic photons, various astronomical observations provide rich data from which one can obtain various constraints for LV researches. Here, we review four common astronomical phenomena which are ideal for LV studies, together with current constraints on LV effects of photons.
Variable Gene Expression in Human Ganglia Latently Infected with Varicella-Zoster Virus
Authors: Kennedy Peter G. E. , Montague Paul
Published: June 2022

Varicella-Zoster virus (VZV) is a pathogenic human herpes virus that causes varicella (“chicken pox”) as a primary infection, following which it becomes latent in neuronal cells in human peripheral ganglia. It may then reactivate to cause herpes zoster (“shingles”). Defining the pattern of VZV gene expression during latency is an important issue, and four highly expressed VZV genes were first identified by Randall Cohrs in 1996 using cDNA libraries. Further studies from both his and other laboratories, including our own, have suggested that viral gene expression may be more widespread than previously thought, but a confounding factor has always been the possibility of viral reactivation after death in tissues obtained even at 24 h post-mortem. Recent important studies, which Randall Cohrs contributed to, have clarified this issue by studying human trigeminal ganglia at 6 h after death using RNA-Seq methodology when a novel spliced latency-associated VZV transcript (VLT) was found to be mapped antisense to the viral transactivator gene 61. Viral gene expression could be induced by a VLT-ORF 63 fusion transcript when VZV reactivated from latency. Prior detection by several groups of ORF63 in post-mortem-acquired TG is very likely to reflect detection of the VLT-ORF63 fusion and not canonical ORF63. The contributions to the VZV latency field by Randall Cohrs have been numerous and highly significant.
Characterization of Chronic Hepatitis E Virus Infection in Immunocompetent Rabbits
Authors: Liang Chunnan , Zhao Chenyan , Liu Tianlong , Liu Bo , Liu Zhiguo , Huang Huili , Liu Wei , Zhao Minghai , Xu Nan , Lu Qiong , Nie Jianhui , Zhang Li , Huang Weijin , She Ruiping , Wang Youchun
Published: June 2022

Chronic hepatitis E virus (HEV) infection is frequently reported in immunocompromised patients, but has also been increasingly reported in non-immunocompromised individuals. We characterized the course of chronic HEV infection in immunocompetent rabbits. In two independent experiments, 40 specific-pathogen-free rabbits were infected with a rabbit HEV genotype 3 strain in serial diluted titers (108 to 104 copies/mL). Serum and fecal samples were collected weekly and were tested for HEV RNA, antigen, anti-HEV and liver enzymes. Rabbits that spontaneously cleared the infection before 10 weeks post-inoculation (wpi) were kept to the end of the study as recovery control. Liver tissues were collected from HEV-infected rabbits at 5, 10 and 26 wpi for histopathological analysis. Nineteen rabbits (47.5%) developed chronic HEV infection with persistent viraemia and fecal HEV shedding for >6 months. Seroconversion to anti-HEV was observed in 84.2% (16/19) of the chronically infected rabbits. Serum levels of aminotransferase were persistently elevated in most of the rabbits. Characterizations of chronic HEV infection in immunocompetent settings could be recapitulated in rabbits, which can serve as a valuable tool for future studies on pathogenesis.
Genome-Wide Reassortment Analysis of Influenza A H7N9 Viruses Circulating in China during 2013–2019
Authors: He Dongchang , Wang Xiyue , Wu Huiguang , Wang Xiaoquan , Yan Yayao , Li Yang , Zhan Tiansong , Hao Xiaoli , Hu Jiao , Hu Shunlin , Liu Xiaowen , Ding Chan , Su Shuo , Gu Min , Liu Xiufan
Published: June 2022

Reassortment with the H9N2 virus gave rise to the zoonotic H7N9 avian influenza virus (AIV), which caused more than five outbreak waves in humans, with high mortality. The frequent exchange of genomic segments between H7N9 and H9N2 has been well-documented. However, the reassortment patterns have not been described and are not yet fully understood. Here, we used phylogenetic analyses to investigate the patterns of intersubtype and intrasubtype/intralineage reassortment across the eight viral segments. The H7N9 virus and its progeny frequently exchanged internal genes with the H9N2 virus but rarely with the other AIV subtypes. Before beginning the intrasubtype/intralineage reassortment analyses, five Yangtze River Delta (YRD A-E) and two Pearl River Delta (PRD A-B) clusters were divided according to the HA gene phylogeny. The seven reset segment genes were also nomenclatured consistently. As revealed by the tanglegram results, high intralineage reassortment rates were determined in waves 2–3 and 5. Additionally, the clusters of PB2 c05 and M c02 were the most dominant in wave 5, which could have contributed to the onset of the largest H7N9 outbreak in 2016–2017. Meanwhile, a portion of the YRD-C cluster (HP H7N9) inherited their PB2, PA, and M segments from the co-circulating YRD-E (LP H7N9) cluster during wave 5. Untanglegram results revealed that the reassortment rate between HA and NA was lower than HA with any of the other six segments. A multidimensional scaling plot revealed a robust genetic linkage between the PB2 and PA genes, indicating that they may share a co-evolutionary history. Furthermore, we observed relatively more robust positive selection pressure on HA, NA, M2, and NS1 proteins. Our findings demonstrate that frequent reassortment, particular reassorted patterns, and adaptive mutations shaped the H7N9 viral genetic diversity and evolution. Increased surveillance is required immediately to better understand the current state of the HP H7N9 AIV.
Molecular Characterisation and Phylogeny of Tula Virus in Kazakhstan
Authors: Tukhanova Nur , Shin Anna , Turebekov Nurkeldi , Nurmakhanov Talgat , Abdiyeva Karlygash , Shevtsov Alexandr , Yerubaev Toktasyn , Tokmurziyeva Gulnara , Berdibekov Almas , Sutyagin Vitaliy , Maikanov Nurbek , Zakharov Andrei , Lezdinsh Ilmars , Yeraliyeva Lyazzat , Froeschl Guenter , Hoelscher Michael , Frey Stefan , Wagner Edith , Peintner Lukas , Essbauer Sandra
Published: June 2022

Orthohantaviruses are zoonotic pathogens that play a significant role in public health. These viruses can cause haemorrhagic fever with renal syndrome in Eurasia. In the Republic of Kazakhstan, the first human cases were registered in the year 2000 in the West Kazakhstan region. Small mammals can be reservoirs of orthohantaviruses. Previous studies showed orthohantavirus antigens in wild-living small mammals in four districts of West Kazakhstan. Clinical studies suggested that there might be further regions with human orthohantavirus infections in Kazakhstan, but genetic data of orthohantaviruses in natural foci are limited. The aim of this study was to investigate small mammals for the presence of orthohantaviruses by molecular biological methods and to provide a phylogenetic characterization of the circulating strains in Kazakhstan. Small mammals were trapped at 19 sites in West Kazakhstan, four in Almaty region and at seven sites around Almaty city during all seasons of 2018 and 2019. Lung tissues of small mammals were homogenized and RNA was extracted. Orthohantavirus RT-PCR assays were applied for detection of partial S and L segment sequences. Results were compared to published fragments. In total, 621 small mammals from 11 species were analysed. Among the collected small mammals, 2.4% tested positive for orthohantavirus RNA, one sample from West Kazakhstan and 14 samples from Almaty region. None of the rodents caught in Almaty city were infected. Sequencing parts of the small (S) and large (L) segments specified Tula virus (TULV) in these two regions. Our data show that geographical distribution of TULV is more extended as previously thought. The detected sequences were found to be split in two distinct genetic clusters of TULV in West Kazakhstan and Almaty region. TULV was detected in the common vole (Microtus arvalis) and for the first time in two individuals of the forest dormouse (Dryomys nitedula), interpreted as a spill-over infection in Kazakhstan.
Tyrosine 146 of the Human Na+/Taurocholate Cotransporting Polypeptide (NTCP) Is Essential for Its Hepatitis B Virus (HBV) Receptor Function and HBV Entry into Hepatocytes
Authors: Zakrzewicz Dariusz , Leidolf Regina , Kunz Sebastian , Müller Simon Franz , Neubauer Anita , Leiting Silke , Goldmann Nora , Lehmann Felix , Glebe Dieter , Geyer Joachim
Published: June 2022

Na+/taurocholate cotransporting polypeptide (NTCP, gene symbol SLC10A1) is a hepatic bile acid uptake carrier participating in the enterohepatic circulation of bile acids. Apart from its transporter function, NTCP acts as the high-affinity liver-specific receptor for the hepatitis B virus (HBV), which attaches via its preS1-peptide domain of the large surface protein to NTCP, subsequently leading to endocytosis of the virus/NTCP-receptor complex. Although the process of NTCP-dependent HBV infection of hepatocytes has received much attention over the last decade, the precise molecular sites of the virus/NTCP interaction have not been fully identified. Inspection of the primary protein sequence of human NTCP revealed 139YIYSRGIY146 as a highly conserved tyrosine-rich motif. To study the role of Y139, Y141 and Y146 amino acids in NTCP biology, the aforementioned residues were substituted with alanine, phenylalanine or glutamate (mimicking phosphorylation) using site-directed mutagenesis. Similar to wt NTCP, the Y139A, Y141A, Y146A, Y141F, Y146F, and Y146E mutants were expressed at the plasma membrane of HEK293 cells and exhibited intact bile acid transport function. Y146A, Y146E, and Y146F demonstrated transport kinetics comparable to wild-type NTCP with Km values of 57.3–112.4 µM and Vmax values of 6683–7579 pmol/mg protein/min. Only Y141E was transport deficient, most likely due to an intracellular accumulation of the mutant protein. Most importantly, Y146A and Y146E mutation completely abrogated binding of the viral preS1-peptide to NTCP, while the Y146F mutant of NTCP showed some residual binding competence for preS1. Consequently, the NTCP mutants Y146A and Y146E, when expressed in HepG2 hepatoma cells, showed complete loss of susceptibility for in vitro HBV infection. In conclusion, tyrosine 146, and to some extent tyrosine 141, both belonging to the tyrosine-rich motif 139YIYSRGIY146 of human NTCP, are newly identified amino acid residues that play an essential role in the interaction of HBV with its receptor NTCP and, thus, in the process of virus entry into hepatocytes.
Sequential Appearance and Isolation of a SARS-CoV-2 Recombinant between Two Major SARS-CoV-2 Variants in a Chronically Infected Immunocompromised Patient
Authors: Burel Emilie , Colson Philippe , Lagier Jean-Christophe , Levasseur Anthony , Bedotto Marielle , Lavrard-Meyer Philippe , Fournier Pierre-Edouard , La Scola Bernard , Raoult Didier
Published: June 2022

Genetic recombination is a major evolutionary mechanism among RNA viruses, and it is common in coronaviruses, including those infecting humans. A few SARS-CoV-2 recombinants have been reported to date whose genome harbored combinations of mutations from different mutants or variants, but only a single patient’s sample was analyzed, and the virus was not isolated. Here, we report the gradual emergence of a hybrid genome of B.1.160 and Alpha variants in a lymphoma patient chronically infected for 14 months, and we isolated the recombinant virus. The hybrid genome was obtained by next-generation sequencing, and the recombination sites were confirmed by PCR. This consisted of a parental B.1.160 backbone interspersed with two fragments, including the spike gene, from an Alpha variant. An analysis of seven sequential samples from the patient decoded the recombination steps, including the initial infection with a B.1.160 variant, then a concurrent infection with this variant and an Alpha variant, the generation of hybrid genomes, and eventually the emergence of a predominant recombinant virus isolated at the end of the patient’s follow-up. This case exemplifies the recombination process of SARS-CoV-2 in real life, and it calls for intensifying the genomic surveillance in patients coinfected with different SARS-CoV-2 variants, and more generally with several RNA viruses, as this may lead to the appearance of new viruses.
Intraneuronal β-Amyloid Accumulation: Aging HIV-1 Human and HIV-1 Transgenic Rat Brain
Authors: Li Hailong , McLaurin Kristen A. , Mactutus Charles F. , Likins Benjamin , Huang Wenfei , Chang Sulie L. , Booze Rosemarie M.
Published: June 2022

The prevalence of HIV-1 associated neurocognitive disorders (HAND) is significantly greater in older, relative to younger, HIV-1 seropositive individuals; the neural pathogenesis of HAND in older HIV-1 seropositive individuals, however, remains elusive. To address this knowledge gap, abnormal protein aggregates (i.e., β-amyloid) were investigated in the brains of aging (>12 months of age) HIV-1 transgenic (Tg) rats. In aging HIV-1 Tg rats, double immunohistochemistry staining revealed abnormal intraneuronal β-amyloid accumulation in the prefrontal cortex (PFC) and hippocampus, relative to F344/N control rats. Notably, in HIV-1 Tg animals, increased β-amyloid accumulation occurred in the absence of any genotypic changes in amyloid precursor protein (APP). Furthermore, no clear amyloid plaque deposition was observed in HIV-1 Tg animals. Critically, β-amyloid was co-localized with neurons in the cortex and hippocampus, supporting a potential mechanism underlying synaptic dysfunction in the HIV-1 Tg rat. Consistent with these neuropathological findings, HIV-1 Tg rats exhibited prominent alterations in the progression of temporal processing relative to control animals; temporal processing relies, at least in part, on the integrity of the PFC and hippocampus. In addition, in post-mortem HIV-1 seropositive individuals with HAND, intraneuronal β-amyloid accumulation was observed in the dorsolateral PFC and hippocampal dentate gyrus. Consistent with observations in the HIV-1 Tg rat, no amyloid plaques were found in these post-mortem HIV-1 seropositive individuals with HAND. Collectively, intraneuronal β-amyloid aggregation observed in the PFC and hippocampus of HIV-1 Tg rats supports a potential factor underlying HIV-1 associated synaptodendritic damage. Further, the HIV-1 Tg rat provides a biological system to model HAND in older HIV-1 seropositive individuals.
Torque Teno Virus Primary Infection Kinetics in Early Childhood
Authors: Väisänen Elina , Kuisma Inka , Mäkinen Marjaana , Ilonen Jorma , Veijola Riitta , Toppari Jorma , Hedman Klaus , Söderlund-Venermo Maria
Published: June 2022

Human torque teno viruses (TTVs) are a diverse group of small nonenveloped viruses with circular, single-stranded DNA genomes. These elusive anelloviruses are harbored in the blood stream of most humans and have thus been considered part of the normal flora. Whether the primary infection as a rule take(s) place before or after birth has been debated. The aim of our study was to determine the time of TTV primary infection and the viral load and strain variations during infancy and follow-up for up to 7 years. TTV DNAs were quantified in serial serum samples from 102 children by a pan-TTV quantitative PCR, and the amplicons from representative time points were cloned and sequenced to disclose the TTV strain diversity. We detected an unequivocal rise in TTV-DNA prevalence, from 39% at 4 months of age to 93% at 2 years; all children but one, 99%, became TTV-DNA positive before age 4 years. The TTV-DNA quantities ranged from 5 × 101 to 4 × 107 copies/mL, both within and between the children. In conclusion, TTV primary infections occur mainly after birth, and increase during the first two years with high intra- and interindividual variation in both DNA quantities and virus strains.
Global Burden of Cyclospora cayetanensis Infection and Associated Risk Factors in People Living with HIV and/or AIDS
Authors: Ramezanzadeh Saba , Beloukas Apostolos , Pagheh Abdol Sattar , Rahimi Mohammad Taghi , Hosseini Seyed Abdollah , Oliveira Sonia M. Rodrigues , de Lourdes Pereira Maria , Ahmadpour Ehsan
Published: June 2022

Cyclospora cayetanensis infections remain one of the most common protozoan opportunistic causes of gastrointestinal diseases and diarrhea among people living with HIV and/or AIDS (PLWHA). This study was conducted to provide a summary of the evidence on the global burden of C. cayetanensis infection and associated risk factors among PLWHA. Scopus, PubMed, Science Direct, and EMBASE were searched up to February 2022. All original peer-reviewed original research articles were considered, including descriptive and cross-sectional studies describing C. cayetanensis in PLWHA. Incoherence and heterogeneity between studies were quantified by I index and Cochran’s Q test. Publication and population bias were assessed with funnel plots and Egger’s asymmetry regression test. All statistical analyses were performed using StatsDirect. The pooled prevalence of C. cayetanensis infection among PLWHA was 3.89% (95% CI, 2.62–5.40). The highest prevalence found in South America was 7.87% and the lowest in Asia 2.77%. In addition, the prevalence of C. cayetanensis was higher in PLWHA compared to healthy individuals. There was a relationship between a higher C. cayetanensis prevalence in PLWHA with a CD4 cell count below 200 cells/mL and people with diarrhea. The results show that PLWHA are more vulnerable to C. cayetanensis infection and emphasizes the need to implement the screening and prophylaxis tailored to the local context. Owing to the serious and significant clinical manifestations of the parasite, an early identification of seropositivity is recommended to initiate prophylaxis between PLWHA with a CD4 count ≤200 cells/mL and PLWHA who do not receive antiviral therapy.
Design of a US28 ORF Deletion Virus in a Temperature-Sensitive Cytomegalovirus Strain Fails to Promote Lytic Replication in Hematopoietic Cells
Authors: Krishna Benjamin A. , Wass Amanda B. , Murphy Eain A. , O’Connor Christine M.
Published: June 2022

Human cytomegalovirus (CMV) is a ubiquitous pathogen that latently resides in hematopoietic cells. Latently infected individuals with dysfunctional immune systems often experience CMV reactivation, which can cause devastating disease and mortality. While factors dictating the balance between latency and reactivation are not completely understood, CMV US28 is required for maintaining latent infection, and viral mutants that alter US28 function result in a lytic-like, rather than latent, infection in hematopoietic cells. In turn, viral lytic factors alter the host cell, making it challenging to characterize the US28-specific changes in the cellular milieu. To circumvent this, we generated a temperature-sensitive TB40/E recombinant virus, TB40/EgfpC510G (tsC510G), into which we engineered an amino acid change at position 510 (C510G) of IE2, as previously described in the CMV Towne strain. Using tsC510G, we then deleted the US28 ORF, termed tsC510G-US28Δ. Consistent with previous findings, tsC510G-US28Δ fails to undergo latency in Kasumi-3 cells at the permissive temperature. However, parallel cultures maintained at the non-permissive temperature showed a significant reduction in infectious center frequency, as measured by limiting dilution assay. Thus, we generated a new US28 mutant virus for use as a tool to study US28-specific changes in latently infected hematopoietic cells in the absence of induced lytic replication.
Parents’ Intentions and Associated Factors to Vaccinating Their Children Aged 12–17 Years with COVID-19 Vaccines: A Cross Sectional Study
Authors: Al-Wutayd Osama , Al-Batanony Manal , Badr Nehad , Abdelwanees Sally
Published: June 2022

No available vaccine against COVID-19 had yet been proven for 12–17-year-olds in Egypt during the study period. This is the first study to assess Egyptian parents’ intentions and associated factors in relation to vaccinating their children with COVID-19 vaccines. A cross-sectional study using a questionnaire was conducted between 17 October and 17 November 2021, via social media platforms. The target group was parents with children aged 12–17 years. Parents’ intention to vaccinate their children and factors associated with vaccinating their children, reasons for not intending to vaccinate their children, and circumstances whereby the parents would change their mind were recorded. Among the 1458 parents recruited, 65.6% were planning to vaccinate their children. The main concerns were fear of the vaccine’s side-effects (68.3%) and conspiracy theories (18%). The factors associated with parents’ intention to vaccinate their children were mother’s older age (40–49 years: aOR = 1.45, 95% CI = 1.05–1.99; ≥50 years: aOR = 2.09, 95% CI = 1.16–3.75), high family income (aOR = 1.99, 95% CI = 1.41–2.79), and children with a history of chronic conditions (aOR = 2.02, 95% CI = 1.25–3.25), while higher mother’s education level was negatively associated (aOR = 0.41, 95% CI = 0.27, 0.64). A comprehensive health education program regarding vaccinating children with COVID-19 vaccines is highly recommended for parents, particularly for young and highly educated mothers, to enhance children vaccination rate when the vaccine becomes available.
Assessment of the Level of Anxiety for COVID-19 Vaccinations
Authors: Świerad Marcin , Świerad Ilona , Szydło Robert , Honisz Grzegorz , Gąsior Mariusz , Kalarus Zbigniew , Dyrbuś Krzysztof
Published: June 2022

Research published especially in the last decade indicates the influence of anxiety on the human decision-making process. This study analyzes the anxiety among individuals who decided to undergo vaccinations for COVID-19. The study assesses that the level of education, especially medical education, age, and gender, had an influence on the level of anxiety in terms of vaccination situations. The STAI self-assessment questionnaire was used. The study was conducted anonymously using the paper-pencil method during two rounds of vaccination; therefore, the respondent sample included mainly medical personnel and elderly people. A total of 898 questionnaires were issued. Age did not affect the trait and state of anxiety, but highly educated people tested during vaccination had a lower anxiety level. Gender had no influence on the trait but did influence the state of anxiety. Overall, women were the group that exhibited a higher level of anxiety than men. Nurses were particularly vulnerable to the negative effects of situational medication in this group.
Kinome Analysis to Define Mechanisms of Adjuvant Action: PCEP Induces Unique Signaling at the Injection Site and Lymph Nodes
Authors: Awate Sunita , Scruten Erin , Mutwiri George , Napper Scott
Published: June 2022

Understanding the mechanism of action of adjuvants through systems biology enables rationale criteria for their selection, optimization, and application. As kinome analysis has proven valuable for defining responses to infectious agents and providing biomarkers of vaccine responsiveness, it is a logical candidate to define molecular responses to adjuvants. Signaling responses to the adjuvant poly[di(sodiumcarboxylatoethylphenoxy)phosphazene] (PCEP) were defined at the site of injection and draining lymph node at 24 h post-vaccination. Kinome analysis indicates that PCEP induces a proinflammatory environment at the injection site, including activation of interferon and IL-6 signaling events. This is supported by the elevated expression of proinflammatory genes (IFNγ, IL-6 and TNFα) and the recruitment of myeloid (neutrophils, macrophages, monocytes and dendritic cells) and lymphoid (CD4+, CD8+ and B) cells. Kinome analysis also indicates that PCEP’s mechanism of action is not limited to the injection site. Strong signaling responses to PCEP, but not alum, are observed at the draining lymph node where, in addition to proinflammatory signaling, PCEP activates responses associated with growth factor and erythropoietin stimulation. Coupled with the significant (p < 0.0001) recruitment of macrophages and dendritic cells to the lymph node by PCEP (but not alum) supports the systemic consequences of the adjuvant. Collectively, these results indicate that PCEP utilizes a complex, multi-faceted MOA and support the utility of kinome analysis to define cellular responses to adjuvants.
Smart Design: Application of an Automatic New Methodology for the Energy Assessment and Redesign of Hybrid Electric Vehicle Mechanical Components
Authors: Previti Umberto , Galvagno Antonio , Risitano Giacomo , Alberti Fabio
Published: June 2022

This work aimed to develop an automatic new methodology based on establishing if a mechanical component, designed for a conventional propulsion system, is also suitable for hybrid electric propulsion. Change in propulsion system leads to different power delivery and vehicle dynamics, which will be reflected in different load conditions acting on the mechanical components. It has been shown that a workflow based on numerical simulations and experimental tests represents a valid approach for the evaluation of the cumulative fatigue damage of a mechanical component. In this work, the front half-shaft of a road car was analyzed. Starting from the acquisition of a speed profile and the definition of a reference vehicle, in terms of geometry and transmission, a numerical model, based on longitudinal vehicle dynamics, was developed for both conventional and hybrid electric transmission. After the validation of the model, the cumulative fatigue damage of the front half-shaft was evaluated. The new design methodology is agile and light; it has been dubbed “Smart Design”. The results show that changing propulsion led to greater fatigue damage, reducing the fatigue life component by 90%. Hence, it is necessary to redesign the mechanical component to make it also suitable for hybrid electric propulsion.
Symblepharon, Ankyloblepharon, and Salt Gland Dysfunction in a Loggerhead Sea Turtle (Caretta caretta)
Authors: Affuso Andrea , Di Palma Cristina , Meomartino Leonardo , Pace Antonino , Montagnaro Serena , Russo Valeria , Mennonna Giuseppina , Micieli Fabiana , Maffucci Fulvio , Hochscheid Sandra , Lamagna Francesco , D’Aquino Ilaria , Lamagna Barbara
Published: June 2022

Adhesions involving the bulbar and the palpebral conjunctiva (Symblepharon) may interfere with tear drainage, cause chronic conjunctivitis, and reduce ocular motility. This condition may be associated with adhesion of the edges of the upper and lower eyelids (ankyloblepharon). The present case describes bilateral symblepharon, ankyloblepharon and salt gland dysfunction in a juvenile Caretta caretta. The loggerhead presented both eyelids swollen, ulcerated, and not separable when rescued. Eye examination was not possible, but ultrasonography showed right bulbar integrity, while the left eye was smaller, with a thicker cornea that had lost its normal doubled lined structure. Surgical dissection of the fibrous adhesions between the palpebral and bulbar conjunctiva, cornea, and third eyelid was performed, and large dacryoliths were removed. The microscopic findings were consistent with chronic keratoconjunctivitis. Ultrastructurally, no virus-like particles were observed. In addition, tissue samples were negative for herpesvirus by qualitative PCR. The eyelids of both eyes and the corneal epithelium of the right eye healed; moreover, the vision was restored in the right eye. There were no recurrences after 12 months of follow-up, and the turtle was released 16 months after the end of treatments on the southern Tyrrhenian coast in the western Mediterranean Sea. To the authors’ knowledge, this is the first report of symblepharon with ankyloblepharon and salt gland dysfunction in Caretta caretta turtle. Ocular ultrasonography was helpful in the preliminary diagnostic work-up.
Tumor-Homing of Mesenchymal Stem Cells Infected with Oncolytic Virus in a Canine Patient
Authors: Delgado-Bonet Pablo , Tomeo-Martín Beatriz Davinia , Ortiz-Díez Gustavo , Perisé-Barrios Ana Judith
Published: June 2022

Intravenous administration of oncolytic adenovirus (OAds) can be challenging, although various vehicles for the delivery of the virus to the tumor have been described. The efficacy of mesenchymal stem cells (MSCs) as a virus vehicle has been reported in mouse models and canine and human patients, but the actual action mechanism has never been described in patients. It is of importance to determine whether MSCs infected with OAds can reach the tumor and release the virus in a clinical setting. For this purpose, GFP-labeled MSCs were infected with an OAd and inoculated into a companion dog diagnosed with spontaneous lung carcinoma. Forty-eight hours later, the tumor was excised and analyzed microscopically by flow cytometry for GFP fluorescence detection, and a cellular culture was established. Peripheral blood samples were taken to quantify the oncolytic adenovirus by qRT-PCR. Green fluorescence cells detected in the cellular culture by microscopy and flow cytometry revealed 0.69% GFP-positive cells in the tumor. OAd in peripheral blood was confirmed by qRT-PCR during follow-up. For the first time, the tumoral-homing capacity of OAds infected-MSC has been confirmed in a clinical setting, helping to explain the clinical response mechanism, whose efficacy was previously reported in canine and human patients.
Experimental Infection of Pigs with a ST 245 Brachyspira hyodysenteriae Isolated from an Asymptomatic Pig in a Herd with No History of Swine Dysentery
Authors: Sato José Paulo H. , Daniel Amanda G. S. , Pereira Carlos E. R. , Andrade Mariana R. , Laub Ricardo P. , Gabardo Michelle P. , Otoni Luisa V. A. , Macedo Nubia R. , Barrera-Zarate Javier A. , Guedes Roberto M. C.
Published: June 2022

Swine dysentery (SD) is characterized by a severe mucohemorrhagic colitis caused by infection with Brachyspira species. In infected herds the disease causes considerable financial loss due to mortality, slow growth rates, poor feed conversion, and costs of treatment. B. hyodysenteriae is the most common etiological agent of SD and infection is usually associated with disease. However, isolated reports have described low pathogenic strains of B. hyodysenteriae. The aim of this study was to describe an experimental infection trial using a subclinical B. hyodysenteriae isolated from an animal without clinical signs and from a disease-free herd, to evaluate the pathogenicity and clinical pathological characteristics compared to a highly clinical isolate. Forty-eight 5-week-old pigs were divided into three groups: control, clinical and the subclinical isolates. The first detection/isolation of B. hyodysenteriae in samples of the animals challenged with a known clinical B. hyodysenteriae strain (clinical group) occurred 5th day post inoculation. Considering the whole period of the study, 11/16 animals from this group were qPCR positive in fecal samples, and diarrhea was observed in 10/16 pigs. In the subclinical isolate group, one animal had diarrhea. There were SD large intestine lesions in 3 animals at necropsy and positive B. hyodysenteriae isolation in 7/15 samples of the subclinical group. In the control group, no diarrhea, gross/microscopic lesions, or qPCR positivity were observed. Clinical signs, bacterial isolation, macroscopic and histologic lesions were significantly difference among groups, demonstrating low pathogenicity of the subclinical isolate in susceptible pigs.
ADSWIM and WATERCARE Projects Meet Kids and Youth: The Challenge of Bringing the World of Research to School to Merge Research, Education and Communication
Authors: Baldrighi Elisa , Muzlovic Patricija , Annibaldi Anna , Penna Antonella , Manini Elena , Rosetti Elia , Renzoni Enrico Esposito , Grilli Federica , Giacomini Gloria , Kristovic Ivana , Duracic Ivo , Krzelj Maja , Ordulj Marin , Bućan Martin , Penna Pierluigi , Spada Vedrana , Bilic Josipa , Marini Mauro , Susmel Sabina
Published: June 2022

The transfer of communication and knowledge from science and research to the general public is a paramount step to raise people’s awareness about environmental issues and their negative and positive impacts on each of us. Many projects and initiatives seek to raise awareness among citizens, with particular attention to young people, about the importance of maintaining clean and healthy oceans. With this paper, we aim to present the successful communication initiatives developed during two Interreg projects, AdSWiM and WATERCARE, with schools and educational organisations on the local and national levels in Italy and Croatia. Both projects make a special effort to realize dedicated communication strategies with the objective of raising the awareness of environmental topics and issues among young people (i.e., students of different school grades) and teachers. The promotion of ocean literacy among students is crucial, as children and young people represent the future citizens and consumers who will develop attitudes and make decisions that will inevitably affect the environment.
A Plea for Considering Processes That Take Place on the Micrometer Scale in Modelling the Groundwater Ecosystems’ Functions
Authors: Schmidt Susanne I.
Published: June 2022

The largest freshwater ecosystem on earth is in the subsurface: the groundwater. It is populated by animals of almost all phyla and by bacteria, archaea, and fungi. Processes on the macro-, meso-, and micro-scale shape this ecosystem. Bioremediation, i.e., the degradation of contaminants, is steered on the scale of micrometers. However, processes that take place on the micrometer scale are still poorly understood and have not been studied extensively. They are usually lacking from models. In this communication, the plea for studying and making models for the processes that take place on the micrometer scale is based on the conceptual model of bottom-up effects of the pore scale environment. Such conceptual models may influence how quantitative models are built by structuring them.
Turbidity and Estimated Phosphorus Retention in a Reconnected Lake Erie Coastal Wetland
Authors: Carter Glenn S. , Kowalski Kurt P. , Eggleston Michael R.
Published: June 2022

Nearly all of the wetlands in the coastal zone of Lake Erie have been degraded or destroyed since the 1860s, and most of those that remain are separated from their watersheds by earthen dikes. Hydrologic isolation of these wetlands disrupts ecosystem benefits typical to Great Lakes coastal wetlands, particularly the ability to trap sediments and retain nutrients when inundated by runoff and lake water. High-frequency measurements of turbidity and discharge were taken in 2013 and 2014 to observe turbidity and water flow dynamics to estimate total phosphorus flux of a hydrologically reconnected diked wetland pool in the Crane Creek-Lake Erie wetland complex. Modeled estimates suggest the reconnected pool retained 8% of the total phosphorus loading in 2013 and 10% in 2014, which included short periods of phosphorus export to Lake Erie. Water flowing out of the wetland generally had lower turbidity than inflowing water, but flux in and out of the pool varied seasonally and was linked to changes in lake-levels, seiche dynamics, and weather conditions. More frequent storms, higher winds, and stronger seiches in the spring and fall created turbidity patterns that suggest more phosphorus retention than in summer or winter. Estimates suggest that phosphorus was released during the summer when higher lake levels and the absence of frequent storms, larger short-term seiche oscillations, and potentially soil oxygen availability were driving flux dynamics. This study demonstrated that reestablishing lake hydrology through reconnection of wetland pools can reduce loading and alter timing of delivery of total phosphorus to Lake Erie.
A Novel Method of Design Flood Hydrographs Estimation for Flood Hazard Mapping
Authors: Gądek Wiesław , Baziak Beata , Tokarczyk Tamara , Szalińska Wiwiana
Published: June 2022

Flood hazard mapping requires knowledge of peak flow as well as flood wave volume and shape, usually represented as a design flood hydrograph (DFH). Statistical approaches for DFH development include nonparametric and parametric methods. The former are developed from long-term flow observations and are thus related to the physio-hydro-climatological catchment properties, but not applicable for ungauged catchments. The alternative parametric DFH can be estimated for any river cross-section, but its links with catchment characteristics are limited. The goal of this study was to introduce a novel hybrid approach for DFH estimation, where the parametric DFH is estimated from the selected properties of the nonparametric DFH (hydrograph width at the levels of 50% and 75% of the peak flow and skewness coefficient) that can be related to the catchment characteristics. The model that offers effective parameter estimation and best correspondence to the reference observation-based hydrograph was selected from among Gamma distribution, Strupczewski and Baptista candidates. The method was validated for 34 catchments of the upper Vistula River and Middle Odra water regions (Poland) based on data from the 1964–2010 period. The Baptista method was found to provide the best model for hybrid DFH construction according to the applied quality measures.
Nanobubble Technology Enhanced Ozonation Process for Ammonia Removal
Authors: Wu Yuncheng , Tian Wei , Zhang Yang , Fan Wei , Liu Fang , Zhao Jiayin , Wang Mengmeng , Liu Yu , Lyu Tao
Published: June 2022

Ozone (O3) has been widely used for water and wastewater treatment due to its strong oxidation ability, however, the utilization efficiency of O3 is constrained by its low solubility and short half-life during the treatment process. Thereby, an integrated approach using novel nanobubble technology and ozone oxidation method was studied in order to enhance the ozonization of ammonia. Artificial wastewater (AW) with an initial concentration of 1600 mg/L ammonia was used in this study. In the ozone-nanobubble treatment group, the concentration of nano-sized bubbles was 2.2 × 107 particles/mL, and the bubbles with <200 nm diameter were 14 times higher than those in the ozone-macrobubble treatment control group. Ozone aeration was operated for 5 min in both nanobubble treatment and control groups, however, the sampling and measurement were conducted for 30 min to compare the utilization of O3 for ammonia oxidation. H+ was the by-product of the ammonia ozonation process, thus the pH decreased from 8 to 7 and 7.5 in nanobubble treatment and control groups, respectively, after 30 min of operation. The fast removal of ammonia was observed in both systems in the first 10 min, where the concentration of ammonia decreased from 1600 mg/L to 835 and 1110 mg/L in nanobubble treatment and control groups, respectively. In the nanobubble treatment group, ammonia concentrations kept the fast-decreasing trend and reached the final removal performance of 82.5% at the end of the experiment, which was significantly higher than that (44.2%) in the control group. Moreover, the first-order kinetic model could be used to describe the removal processes and revealed a significantly higher kinetic rate constant (0.064 min−1) compared with that (0.017 min−1) in the control group. With these results, our study highlights the viability of the proposed integrated approach to enhance the ozonation of a high level of ammonia in contaminated water.