bluuebunny/crossref_metadata_2025 huggingface dataset:
Case report: Novel SIN3A loss-of-function variant as causative for hypogonadotropic hypogonadism in Witteveen–Kolk syndrome
Authors: Correa Brito Lourdes , Keselman Ana , Villegas Florencia , Scaglia Paula , Esnaola Azcoiti María , Castro Sebastián , Sanguineti Nora , Izquierdo Agustín , Maier Marianela , Bergadá Ignacio , Arberas Claudia , Rey Rodolfo A. , Ropelato María Gabriela
Published: March 2024

Pubertal delay can be due to hypogonadotropic hypogonadism (HH), which may occur in association with anosmia or hyposmia and is known as Kallmann syndrome (OMIM #308700). Recently, hypogonadotropic hypogonadism has been suggested to overlap with Witteveen–Kolk syndrome (WITKOS, OMIM #613406) associated with 15q24 microdeletions encompassing SIN3A. Whether hypogonadotropic hypogonadism is due to haploinsufficiency of SIN3A or any of the other eight genes present in 15q24 is not known. We report the case of a female patient with delayed puberty associated with intellectual disability, behavior problems, dysmorphic facial features, and short stature, at the age of 14 years. Clinical, laboratory, and imaging assessments confirmed the diagnosis of Kallmann syndrome. Whole-exome sequencing identified a novel heterozygous frameshift variant, NM_001145358.2:c.3045_3046dup, NP_001138830.1:p.(Ile1016Argfs*6) in SIN3A, classified as pathogenic according to the American College of Medical Genetics and Genomics (ACMG/AMP) criteria. Reverse phenotyping led to the clinical diagnosis of WITKOS. No other variant was found in the 96 genes potentially related to hypogonadotropic hypogonadism. The analysis of the other contiguous seven genes to SIN3A in 15q24 did not reveal any clinically relevant variant. In conclusion, these findings point to SIN3A as the gene in 15q24 related to the reproductive phenotype in patients with overlapping WITKOS and Kallmann syndrome.
Comparative analysis of single-cell transcriptome reveals heterogeneity and commonality in the immune microenvironment of colorectal cancer and inflammatory bowel disease
Authors: Lv Hongchao , Mu Yu , Zhang Chen , Zhao Meiqi , Jiang Ping , Xiao Shan , Sun Haiming , Wu Nan , Sun Donglin , Jin Yan
Published: March 2024

Background

During aging, chronic inflammation can promote tumor development and metastasis. Patients with chronic inflammatory bowel diseases (IBD) are at an increased risk of developing colorectal cancer (CRC). However, the molecular mechanism underlying is still unclear.

Methods

We conducted a large-scale single-cell sequencing analysis comprising 432,314 single cells from 92 CRC and 24 IBD patients. The analysis focused on the heterogeneity and commonality of CRC and IBD with respect to immune cell landscape, cellular communication, aging and inflammatory response, and Meta programs.

Results

The CRC and IBD had significantly different propensities in terms of cell proportions, differential genes and their functions, and cellular communication. The progression of CRC was mainly associated with epithelial cells, fibroblasts, and monocyte-macrophages, which displayed pronounced metabolic functions. In particular, monocyte-macrophages were enriched for the aging and inflammation-associated NF-κB pathway. And IBD was enriched in immune-related functions with B cells and T cells. Cellular communication analysis in CRC samples displayed an increase in MIF signaling from epithelial cells to T cells, and an increase in the efferent signal of senescence-associated SPP1 signaling from monocyte-macrophages. Notably, we also found some commonalities between CRC and IBD. The efferent and afferent signals showed that the pro-inflammatory cytokine played an important role. And the activity of aging and inflammatory response with AUCell analysis also showed a high degree of commonality. Furthermore, using the Meta programs (MPs) with the NMF algorithm, we found that the CRC non-malignant cells shared a substantial proportion of the MP genes with CRC malignant cells (68% overlap) and IBD epithelial cells (52% overlap), respectively. And it was extensively involved in functions of cell cycle and immune response, revealing its dual properties of inflammation and cancer. In addition, CRC malignant and non-malignant cells were enriched for the senescence-related cell cycle G2M phase transition and the p53 signaling pathway.

Conclusion

Our study highlights the characteristics of aging, inflammation and tumor in CRC and IBD at the single-cell level, and the dual property of inflammation-cancer in CRC non-malignant cells may provide a more up-to-date understanding of disease transformation.
Comprehensive analysis of lung macrophages and dendritic cells in two murine models of allergic airway inflammation reveals model- and subset-specific accumulation and phenotypic alterations
Authors: Camp Belinda , Jorde Ilka , Sittel Franka , Pausder Alexander , Jeron Andreas , Bruder Dunja , Schreiber Jens , Stegemann-Koniszewski Sabine
Published: March 2024

Introduction

Allergic asthma has been mainly attributed to T helper type 2 (Th2) and proinflammatory responses but many cellular processes remain elusive. There is increasing evidence for distinct roles for macrophage and dendritic cell (DC) subsets in allergic airway inflammation (AAI). At the same time, there are various mouse models for allergic asthma that have been of utmost importance in identifying key inflammatory pathways in AAI but that differ in the allergen and/or route of sensitization. It is unclear whether and how the accumulation and activation of specialized macrophage and DC subsets depend on the experimental model chosen for analyses.

Methods

In our study, we employed high-parameter spectral flow cytometry to comprehensively assess the accumulation and phenotypic alterations of different macrophage- and DC-subsets in the lung in an OVA- and an HDM-mediated mouse model of AAI.

Results

We observed subset-specific as well as model-specific characteristics with respect to cell numbers and functional marker expression. Generally, alveolar as opposed to interstitial macrophages showed increased MHCII surface expression in AAI. Between the models, we observed significantly increased numbers of alveolar macrophages, CD103+ DC and CD11b+ DC in HDM-mediated AAI, concurrent with significantly increased airway interleukin-4 but decreased total serum IgE levels. Further, increased expression of CD80 and CD86 on DC was exclusively detected in HDM-mediated AAI.

Discussion

Our study demonstrates a model-specific involvement of macrophage and DC subsets in AAI. It further highlights spectral flow cytometry as a valuable tool for their comprehensive analysis under inflammatory conditions in the lung.
Residual brewer’s Saccharomyces cerevisiae yeasts as biofertilizers in horticultural seedlings: towards a sustainable industry and agriculture
Authors: Vargas Mercedes Fabiana , Mestre María Victoria , Vergara Cristina , Maturano Paola , Petrignani Diego , Pesce Virginia , Vazquez Fabio
Published: March 2024

The food industry generates substantial amounts of organic waste often underutilized within the system. Craft beer production, experiencing global rapid expansion, contributes to this waste stream with byproducts such as spent grain, trub, and yeast. Many craft beer industries discharge yeast residue directly into public water bodies. In recent years, yeasts have garnered attention for their potential to enhance plant growth and contribute to sustainable agriculture. This study focuses on characterizing Saccharomyces cerevisiae yeast collected at the end of the craft beer fermentation process. Biomass characterization was conducted, and the yeast’s effect on lettuce and tomato seeds and seedlings was evaluated at four concentrations (105, 106, 107, and 108 cells mL−1) in sterile substrate. After 28 days, plant height, leaf number, fresh and dry weights of both aboveground and root parts, as well as chlorophyll content, were analyzed. The most effective concentration (107 cells mL−1) was applied to tomato seedlings in sterile substrate, compared with a commercial organic fertilizer. After 21 days, growth parameters were assessed. The study demonstrated that increasing yeast doses up to 108 cells mL−1 positively affects seed germination and seedling development. Notably, a dose of 107 cells mL−1 proved effective for application in seedlings as an organic amendment and substitute for commercial products. This integrated approach showcases the potential of yeasts in sustainable agriculture, utilizing byproducts from the food industry to enhance crop performance and mitigate environmental pollution.
Research for development: evidence-based hilsa management improvements in Myanmar
Authors: Akester Michael , Bladon Annabelle , Conallin John
Published: March 2024

The hilsa (Tenualosa ilisha) fishery in Myanmar is of importance as it provides an income for around 1.6 million artisanal low-income fisher households and generated USD 237.32M in export earnings from 156,000 metric tons in 2021. Fisheries Performance Indicator assessments have shown that the fishery, both artisanal and industrial, is close to economic collapse. Research was undertaken to find ways to improve the management of the fishery including studies to establish the migration routes and spawning periodicity of hilsa in addition to socioeconomic assessments and business case valuations to recommend finance mechanisms for improved management and maintenance of the fishery. Results indicate that the closed season for hilsa fishing misses the main spawning period in September. In addition, the chemical analysis of hilsa otoliths shows the importance of upstream spawning grounds – some 800km from the sea. Recommendations were made, and enacted March 2022, regarding the need for a new September closed season in addition to no-take hilsa sanctuaries along the main migration routes. A value was placed on the hilsa fishery at close to USD800M per annum. A USD100M annual investment cost is suggested to ensure that the new and old closed seasons are respected by licensed and unlicensed fishers. This investment could be generated by recommended fiscal reforms to provide incentives for fishers to respect the new fishing restrictions. Transboundary fisheries management opportunities are being advanced by informal tripartite scientific collaboration between Bangladesh, India and Myanmar. Social aspects, involving fisheries co-management with artisanal fisher communities and local government authorities, need to be continued to demonstrate the benefits of improved fisheries management in terms of the expected sustainable yields and a move away from potential economic fishery collapse.
Biologically Important Areas II for cetaceans within U.S. and adjacent waters - West Coast Region
Authors: Calambokidis John , Kratofil Michaela A. , Palacios Daniel M. , Lagerquist Barbara A. , Schorr Gregory S. , Hanson M. Bradley , Baird Robin W. , Forney Karin A. , Becker Elizabeth A. , Rockwood R. Cotton , Hazen Elliott L.
Published: March 2024

Here we update U.S. West Coast Biologically Important Areas (BIAs) that were published in 2015 using new data and approaches. Additionally, BIAs were delineated for two species that were not delineated in the 2015 BIAs: fin whales and Southern Resident killer whales (SRKW). While harbor porpoise BIAs remained the same, substantial changes were made for other species including identifying both larger overall areas (parent BIAs) and smaller core areas (child BIAs). For blue, fin, and humpback whales we identified, delineated, and scored BIAs using the overlap between the distribution and relative density from three data sources, leveraging the strengths and weaknesses of these approaches: 1) habitat density models based on Southwest Fisheries Science Center (SWFSC) line-transect data from systematic ship surveys conducted through 2018, 2) satellite tag data from deployments conducted by three research groups, and 3) sightings of feeding behavior from non-systematic effort mostly associated with small-boat surveys for photo-identification conducted by Cascadia Research Collective. While the previous BIAs were based solely on a more subjective assignment from only the small boat sightings, here we incorporate the other two data sources and use a more rigorous, quantitative approach to identify higher density areas and integrate the data types. This resulted in larger, better-supported, objective BIAs compared to the previous effort. Our methods are also more consistent with the delineation of BIAs in other regions. For SRKWs, the parent BIA was based on a modification of the Critical Habitat boundaries defined by the National Oceanographic and Atmospheric Administration (NOAA) and the Department of Fisheries and Oceans (DFO) Canada; a core BIA highlighting areas of intensified use was identified using both NOAA’s Critical Habitat and kernel density analyses of satellite tag data. Gray whale BIAs were re-evaluated for the migratory corridor of Eastern North Pacific gray whales, for Pacific Coast Feeding Group feeding areas, and for gray whales that feed regularly in Puget Sound.
Addressing bias in the definition of SARS-CoV-2 reinfection: implications for underestimation
Authors: Chemaitelly Hiam , Ayoub Houssein H. , Tang Patrick , Yassine Hadi M. , Al Thani Asmaa A. , Hasan Mohammad R. , Coyle Peter , Al-Kanaani Zaina , Al-Kuwari Einas , Jeremijenko Andrew , Kaleeckal Anvar Hassan , Latif Ali Nizar , Shaik Riyazuddin Mohammad , Abdul-Rahim Hanan F. , Nasrallah Gheyath K. , Al-Kuwari Mohamed Ghaith , Butt Adeel A. , Al-Romaihi Hamad Eid , Al-Thani Mohamed H. , Al-Khal Abdullatif , Bertollini Roberto , Abu-Raddad Laith J.
Published: March 2024

Introduction

Reinfections are increasingly becoming a feature in the epidemiology of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. However, accurately defining reinfection poses methodological challenges. Conventionally, reinfection is defined as a positive test occurring at least 90 days after a previous infection diagnosis. Yet, this extended time window may lead to an underestimation of reinfection occurrences. This study investigated the prospect of adopting an alternative, shorter time window for defining reinfection.

Methods

A longitudinal study was conducted to assess the incidence of reinfections in the total population of Qatar, from February 28, 2020 to November 20, 2023. The assessment considered a range of time windows for defining reinfection, spanning from 1 day to 180 days. Subgroup analyses comparing first versus repeat reinfections and a sensitivity analysis, focusing exclusively on individuals who underwent frequent testing, were performed.

Results

The relationship between the number of reinfections in the population and the duration of the time window used to define reinfection revealed two distinct dynamical domains. Within the initial 15 days post-infection diagnosis, almost all positive tests for SARS-CoV-2 were attributed to the original infection. However, surpassing the 30-day post-infection threshold, nearly all positive tests were attributed to reinfections. A 40-day time window emerged as a sufficiently conservative definition for reinfection. By setting the time window at 40 days, the estimated number of reinfections in the population increased from 84,565 to 88,384, compared to the 90-day time window. The maximum observed reinfections were 6 and 4 for the 40-day and 90-day time windows, respectively. The 40-day time window was appropriate for defining reinfection, irrespective of whether it was the first, second, third, or fourth occurrence. The sensitivity analysis, confined to high testers exclusively, replicated similar patterns and results.

Discussion

A 40-day time window is optimal for defining reinfection, providing an informed alternative to the conventional 90-day time window. Reinfections are prevalent, with some individuals experiencing multiple instances since the onset of the pandemic.
Therapeutic outcome of patients with Lennox–Gastaut syndrome with mitochondrial respiratory chain complex I deficiency
Authors: Na Ji-Hoon , Lee Young-Mock
Published: March 2024

Background

Lennox–Gastaut syndrome (LGS), a severe developmental epileptic encephalopathy, has various underlying causes. Mitochondrial respiratory chain complex I (MRC I) deficiency is an important cause of metabolic disorders such as mitochondrial dysfunction that can compromise brain function, thereby causing intractable epilepsy, including LGS. Thus, it can be expected that the presence or absence of MRC I deficiency may affect the treatment outcome of patients with LGS.

Objectives

In this retrospective study, we aimed to investigate differences in the epilepsy characteristics and treatment outcomes between patients with LGS with and without MRC I deficiency.

Methods

We retrospectively reviewed the medical records of 92 patients with LGS. We divided 68 patients with LGS according to the presence (n = 30) or absence (n = 38) of MRC I deficiency and compared their epilepsy characteristics.

Results

Generalized tonic and drop seizures were significantly worse in patients with LGS and MRC I deficiency than in those without MRC I deficiency group at the 1-year follow-up (p < 0.001) and final follow-up 1 (p < 0.001). Patients with LGS and MRC I deficiency had significantly fewer electroencephalogram (EEG) improvements compared to those without MRC I deficiency at the 1-year follow-up (p = 0.031). Additionally, in the final follow-up period, patients with LGS and MRC I deficiency had significantly less improvement in EEG findings compared to patients without MRC I deficiency (p < 0.001).

Conclusion

The overall treatment prognosis—in terms of improvement in traumatic generalized tonic seizure, drop seizure, and EEG findings—is worse in patients with LGS and MRC I deficiency than that in patients with LGS but without MRC I deficiency. Additional and targeted treatment is required to treat LGS with MRC I deficiency.
Therapeutic potential to target sialylation and SIGLECs in neurodegenerative and psychiatric diseases
Authors: Wißfeld Jannis , Abou Assale Tawfik , Cuevas-Rios German , Liao Huan , Neumann Harald
Published: March 2024

Sialic acids, commonly found as the terminal carbohydrate on the glycocalyx of mammalian cells, are pivotal checkpoint inhibitors of the innate immune system, particularly within the central nervous system (CNS). Sialic acid-binding immunoglobulin-like lectins (SIGLECs) expressed on microglia are key players in maintaining microglial homeostasis by recognizing intact sialylation. The finely balanced sialic acid-SIGLEC system ensures the prevention of excessive and detrimental immune responses in the CNS. However, loss of sialylation and SIGLEC receptor dysfunctions contribute to several chronic CNS diseases. Genetic variants of SIGLEC3/CD33, SIGLEC11, and SIGLEC14 have been associated with neurodegenerative diseases such as Alzheimer’s disease, while sialyltransferase ST8SIA2 and SIGLEC4/MAG have been linked to psychiatric diseases such as schizophrenia, bipolar disorders, and autism spectrum disorders. Consequently, immune-modulatory functions of polysialic acids and SIGLEC binding antibodies have been exploited experimentally in animal models of Alzheimer’s disease and inflammation-induced CNS tissue damage, including retinal damage. While the potential of these therapeutic approaches is evident, only a few therapies to target either sialylation or SIGLEC receptors have been tested in patient clinical trials. Here, we provide an overview of the critical role played by the sialic acid-SIGLEC axis in shaping microglial activation and function within the context of neurodegeneration and synaptopathies and discuss the current landscape of therapies that target sialylation or SIGLECs.
A recognition of exosomes as regulators of epigenetic mechanisms in central nervous system diseases
Authors: Hu Shunxin , Feng Lei , Yang Zhonghong , Fan Xuechen , Gao Haozheng , Yang Tiancai
Published: March 2024

Exosomes, vesicular structures originating from cells, participate in the conveyance of proteins and nucleic acids. Presently, the centrality of epigenetic modifications in neurological disorders is widely acknowledged. Exosomes exert influence over various epigenetic phenomena, thereby modulating post-transcriptional regulatory processes contingent upon their constituent makeup. Consequently, the heightened attention directed toward exosomes as instigators of epigenetic alterations has burgeoned in recent years. Notably, exosomes serve as vehicles for delivering methyltransferases to recipient cells. More significantly, non-coding RNAs, particularly microRNAs (miRNAs), represent pivotal contents within exosomes, wielding the capacity to influence the expression of diverse factors within the cerebral milieu. The transfer of these exosomal contents amidst brain cells, encompassing neuronal cells and microglia, assumes a critical role in the genesis and progression of neurological disorders, also, this role is not limited to neurological disorders, it may deal with any human disease, such as cancer, and cardiovascular diseases. This review will concentrate on elucidating the regulation of exosome-induced epigenetic events and its subsequent ramifications for neurological diseases. A more profound comprehension of the involvement of exosome-mediated epigenetic regulation in neurological disorders contributes to a heightened awareness of the etiology and advancement of cerebral afflictions.
Broadband directional filter in multilayer liquid crystal polymer films at W-band
Authors: Wang Mengfa , Wang Yiming , Qian Jun , Li Zhaolin , Xue Wenhui , Qi Victor , Zhang Xijian , Ling Haotian , Tang Gongbin , Wang Qingpu , Song Aimin , Cao Chao , Zhang Yifei
Published: March 2024

In this work, a four-port directional filter (DF) with a broad passband and low reflection is proposed at the W-band, which comprises three unit filters at 92, 95, and 97 GHz, cascaded in series. Each unit consists of two microstrip lines in the top circuit layer for signal input and output, two pairs of apertures in the middle ground layer for directional coupling, and one square loop in the bottom layer as a selective resonator. By sweeping the working frequencies of the three units and optimizing the phase delays between them, the proposed filter achieves a 3-dB bandwidth as broad as 16%, an insertion loss of 2.5 dB at 95 GHz, and an out-of-band rejection of −28 and −23 dB at 80 and 110 GHz, respectively. The corresponding reflection attenuation is larger than 9.6 dB from 60 to 105 GHz. To verify our design, a prototype is fabricated and characterized, and its experimental data are consistent with the simulation. This work significantly expands the bandwidth of DFs and may find many applications in frequency division multiplexing and high-gain wireless systems.
Does physical activity influence health behavior, mental health, and psychological resilience under the moderating role of quality of life?
Authors: Liu Ru , Menhas Rashid , Saqib Zulkaif Ahmed
Published: March 2024

Background

Physical activity significantly influences health-related behaviors, encompassing physical and mental well-being. Physical activity has been linked to enhancing health behavior, mental health, and psychological resilience. The current study is based on participants who were active in physical activity to improve health and well-being.

Objectives

To examine the influences of physical activity on health behavior, mental health, and psychological resilience, considering the moderating role of quality of life.

Method

A thorough cross-sectional online survey was conducted from April 15, 2023, to October 15, 2023. The survey was comprehensive and lasted for six months. The online poll received more than one thousand responses under convenience sampling. The selection criteria for the study were above 21 years old, and participants were active in physical activity to improve health and well-being. The collected data were analyzed using appropriate statistical SPSS-25 and SmartPLS 4.0 software to investigate the proposed research paradigm.

Results

SEM results of model 1 (direct coefficients) show that PA has a positive effect on HeB, MeH, PsR, HeB on MeH, HeB on PsR. Out of six (in model 2), four moderating effects of QOL were significant, and two were statistically insignificant.

Conclusion

It has been observed that the quality of life has a moderating role in the relationships between physical exercise and several aspects, such as psychological resilience, mental health, and health-related behavior. It is imperative to emphasize the importance of fostering consistent engagement in physical activity to cultivate a well-balanced and health-conscious way of life.
Assessing anxiety symptom severity in Rwandese adolescents: cross-gender measurement invariance of GAD-7
Authors: Niwenahisemo Lisa Cynthia , Hong Su , Kuang Li
Published: March 2024

Background

Anxiety disorders are among the most common mental health problems experienced by adolescents worldwide because of their evident significant impact on their quality of life and functioning. The generalized anxiety disorder item (GAD-7) was manufactured to identify the severity of self-reported anxiety symptoms. Efforts to address and screen for mental health problems in Rwanda have been limited, and the importance of screening for anxiety disorders is high. The primary aim of this study was to analyze the psychometric properties of the Kinyarwanda version of the Generalized Anxiety Disorder GAD-7, and then test the measurement invariance of the GAD-7 by gender.

Methods

We used the Rwandese version of GAD-7 among secondary school students in Kigali city (n=1813). Measurement invariance of the GAD-7 across gender and report on anxiety symptom severity prevalence. Exploratory factor analysis (EFA) and confirmatory factor analysis (CFA) were used to examine measurement invariance.

Results

Our findings demonstrated that in the sample of 1813 adolescents aged between 12 and 17 years, generalized anxiety symptoms prevalence rates were higher in females (46.4%) than males (n= 29.8%) GAD-7 demonstrated good reliability and validity coefficients with a Cronbach’s α of .077 and KMO and Bartlett test of Sphericity = 0.835. In addition to these psychometric properties, the GAD-7 screening scale had equivalence for configural and metric invariance across groups with excellent fit indices, and we confirmed partial scalar invariance across groups.

Conclusion

The GAD-7 can be used in cross-group comparison of generalized anxiety disorder prevalence, and we acknowledge that full scalar invariance is generally difficult to confirm, especially due to gender differences. We recommend that future studies further investigate populations living in rural areas and conduct trials that will focus on anxiety-specific treatment in Rwandan Clinical health care centers to determine the diagnostic accuracy of this screening tool.
Assessing social protection influence on health status in the European Union
Authors: Bocean Claudiu George , Vărzaru Anca Antoaneta
Published: March 2024

Introduction

Health status and access to healthcare services are crucial factors that directly impact the well-being of individuals and societies. In the European Union (EU), social protection measures are significant in supporting citizens’ health and providing access to healthcare resources.

Methods

This study investigates the relationship between social protection and health status in EU countries. We collected data from Eurostat on the EU member states’ health status, healthcare expenditure, and social protection expenditure. The paper used structural equation modeling (SEM) and cluster analysis to analyze the complex interplay among these variables.

Results

Findings revealed a strong positive correlation between EU countries’ social protection expenditure and healthcare status. Higher social protection spending was associated with improved access to healthcare services and facilities. Moreover, the analysis showed that countries with higher social protection expenditure tended to exhibit better overall health status indicators among their populations.

Discussion

The results suggest that adequate social protection expenditure positively influences health status in the European Union. By investing in robust social protection programs, governments can enhance citizens’ access to healthcare services and resources, ultimately leading to improved health outcomes. These findings underscore the importance of prioritizing social protection policies to address health disparities and promote public health in the EU.
Physiological, anthropometric and athletic performance adaptations from completing a 1-month pre-season period. A two-year longitudinal study in female collegiate soccer players
Authors: Perrotta Andrew S. , Day Brent D. , Correa Camila J. , Scott Anika J. , Ramos Jennifer , Gnatiuk Elizabeth A. , Warburton Darren E. R.
Published: March 2024

Introduction

Collegiate coaches and integrative support staff often utilize pre-season as a brief and intense training period to prepare athletes technically, tactically, and physiologically, to meet the demands of competition during a soccer season. This study sought to examine the dose-response from performing on-field soccer activities during a four-week pre-season period in female collegiate soccer players, and if the magnitude in response was associated with accumulated exercise stress.

Methods

A total of twenty-seven healthy female soccer players training as part of a collegiate soccer program volunteered to participate in this two-year longitudinal study. Data collection commenced prior to the start of each pre-season period, at the beginning of August, and was completed at the beginning of September, when pre-season ended. Data collection periods were separated by a 31-day period. Indices of cardiovascular function, anthropometry, and athletic performance were examined during each data collection period. Internal and external measures of accumulated exercise stress were recorded using the Polar Team Pro® system.

Results

When comparing the beginning to the end of pre-season, significant improvements were observed in body fat (%) [24.2 ± 6.0 “vs.” 23.3 ± 5.6, p = 0.001], heart rate variability (rMSSD) [51.8 ± 25.1 “vs.” 67.9 ± 34.6 ms, p = 0.002], resting heart (bpm) [73.8 ± 12.1 “vs.” 64.3 ± 8.8, p = 0.001] and cardiorespiratory performance (YoYo IRTL-1) [925.8 ± 272.8 “vs.” 1,062.6 ± 223.3 m, p = 0.001]. Significant reductions in musculoskeletal performance were observed through vertical jump height (cm) [24.9 ± 23.7, p = 0.04]. Change in the end of pre-season body weight (kg) was significantly associated with accumulated accelerations and decelerations [r ≥ 0.49, p = 0.01]. End of pre-season change in cardiorespiratory performance was significantly associated with both accumulated training load (au) and TRIMP (au) [r ≥ 0.63, p = 0.01].

Discussion

In conclusion, performing a four-week pre-season period, involving only on-field training, can promote positive and significant adaptations in anthropometry, cardiovascular function, and athletic performance measures in female collegiate soccer players. The magnitudes of these adaptations were associated with both internal and external measures of accumulated exercise stress.
A New Model for Ranking Schools of Public Health: The Public Health Academic Ranking
Authors: Dugerdil Adeline , Babington-Ashaye Awa , Bochud Murielle , Chan Margaret , Chiolero Arnaud , Gerber-Grote Andreas , Künzli Nino , Paradis Gilles , Puhan Milo Alan , Suggs L. Suzanne , Van der Horst Klazine , Escher Gérard , Flahault Antoine
Published: March 2024

Objectives: As there is no ranking designed for schools of Public Health, the aim of this project was to create one.

Methods: To design the Public Health Academic Ranking (PHAR), we used the InCites Benchmarking and Analytics™ software and the Web Of Science™ Core Collection database. We collected bibliometric data on 26 schools of Public Health from each continent, between August and September 2022. We included 11 research indicators/scores, covering four criteria (productivity, quality, accessibility for readers, international collaboration), for the period 2017–2021. For the Swiss School of Public Health (SSPH+), a network gathering faculties across different universities, a specific methodology was used, with member-specific research queries.

Results: The five top schools of the PHAR were: London School of Hygiene and Tropical Medicine, Public Health Foundation of India, Harvard T.H. Chan School of Public Health, SSPH+, Johns Hopkins Bloomberg School of Public Health.

Conclusion: The PHAR allows worldwide bibliometric ordering of schools of Public Health. As this is a pilot project, the results must be taken with caution. This article aims to critically discuss its methodology and future improvements.
Survival Advantage Comparing Older Living Donor Versus Standard Criteria Donor Kidney Transplants
Authors: Patel Kamlesh , Brotherton Anna , Chaudhry Daoud , Evison Felicity , Nieto Thomas , Dabare Dilan , Sharif Adnan
Published: March 2024

The aim of this analysis was to explore mortality outcomes for kidney transplant candidates receiving older living donor kidneys (age ≥60 years) versus younger deceased donors or remaining on dialysis. From 2000 to 2019, all patients on dialysis listed for their first kidney-alone transplant were included in a retrospective cohort analysis of UK transplant registry data. The primary outcome was all-cause mortality, with survival analysis conducted by intention-to-treat principle. Time-to-death from listing was modelled using nonproportional hazard Cox regression models with transplantation handled as a time-dependent covariate. A total of 32,978 waitlisted kidney failure patients formed the primary study cohort, of whom 18,796 (58.5%) received a kidney transplant (1,557 older living donor kidneys and 18,062 standard criteria donor kidneys). Older living donor kidney transplantation constituted only 17.0% of all living donor kidney transplant activity (overall cohort; n = 9,140). Recipients of older living donor kidneys had reduced all-cause mortality compared to receiving SCD kidneys (HR 0.904, 95% CI 0.845–0.967, p = 0.003) and much lower all-cause mortality versus remaining on the waiting list (HR 0.160, 95% CI 0.149–0.172, p < 0.001). Older living kidney donors should be actively explored to expand the living donor kidney pool and are an excellent treatment option for waitlisted kidney transplant candidates.
Deep-Shallow Metaclassifier with Synthetic Minority Oversampling for Anomaly Detection in a Time Series
Authors: Reshadi MohammadHossein , Li Wen , Xu Wenjie , Omashor Precious , Dinh Albert , Xiao Jun , Dick Scott , She Yuntong , Lipsett Michael
Published: March 2024

Anomaly detection in data streams (and particularly time series) is today a vitally important task. Machine learning algorithms are a common design for achieving this goal. In particular, deep learning has, in the last decade, proven to be substantially more accurate than shallow learning in a wide variety of machine learning problems, and deep anomaly detection is very effective for point anomalies. However, deep semi-supervised contextual anomaly detection (in which anomalies within a time series are rare and none at all occur in the algorithm’s training data) is a more difficult problem. Hybrid anomaly detectors (a “normal model” followed by a comparator) are one approach to these problems, but the separate loss functions for the two components can lead to inferior performance. We investigate a novel synthetic-example oversampling technique to harmonize the two components of a hybrid system, thus improving the anomaly detector’s performance. We evaluate our algorithm on two distinct problems: identifying pipeline leaks and patient-ventilator asynchrony.
Impact of Uncertain Flight Time on Heterogeneous UAVs’ Task Planning with Temporal Constraints
Authors: Wang Jianfeng , Jia Gaowei , Guo Zheng , Hou Zhongxi
Published: March 2024

Heterogeneous multi-UAV systems offer distinct advantages through their complementary and coordinated use of their diverse capabilities. However, this complexity poses significant challenges in task planning, particularly in considering temporal constraints among tasks. As task dependencies evolve from simple linear chains to complex networked associations, uncertainties in flight times can have a substantial impact on the overall schedule. To address these challenges, this study introduces a rapid estimation method that recursively calculates task completion times, derives their probability distributions, and assesses the robustness of the plan. Furthermore, a neighborhood search algorithm guided by dynamic time windows is designed to effectively evaluate the consequences of task insertions, precisely to adjust high-risk tasks, and reduce blindness in enumerative neighborhood exploration. Simulation results demonstrate that the proposed approach effectively accounts for inherent randomness in the problem and exhibits strong adaptability to changes in the problem scale, flight time fluctuations, and variations in time window constraints.
Numerical Investigation on the Mechanism of Solid Rocket Motor Instability Induced by Differences between On-Ground and In-Flight Conditions
Authors: Wang Ge , Li Chengke , Pu Weiqiang , Zhou Bocheng , Yang Haiwei , Yang Zenan
Published: March 2024

A solid rocket motor (SRM) with a high aspect ratio that performs normally during ground tests may experience instability during flight. To address this issue, this study employs the pulse triggering method and the numerical approach of two-way fluid–structure interaction to investigate the mechanisms behind the SRM instability resulting from distinctions between on-ground and in-flight conditions. The results indicate that the main distinctions between the on-ground and in-flight conditions for SRMs are the strong constraints during the ground test, as well as aerodynamic forces and aerodynamic heating during flight. The strong constraints in the ground test effectively suppress structural vibrations by limiting displacements. In flight conditions, the aerodynamic heating reduces the strength of the SRM casing and aerodynamic forces provide sustained energy input for structural vibrations during flight. The mechanism for the ground/flight differences that induce SRM instability is that the structural natural frequencies are reduced by aerodynamic heating and the first-order acoustic frequency increased by the propellant regression approach reaches the resonance condition. Therefore, an instability factor Φ is proposed to represent the resonance relationship between the structural natural modes and the acoustic mode of SRMs. Furthermore, the closer the frequency of the aerodynamic forces is to the resonance frequency of the acoustic-structure coupling, the more pronounced the SRM instability.
An Integrated Monitoring, Diagnostics, and Prognostics System for Aero-Engines under Long-Term Performance Deterioration
Authors: Pérez-Ruiz Juan Luis , Tang Yu , Loboda Igor , Miró-Zárate Luis Angel
Published: March 2024

In the field of aircraft engine diagnostics, many advanced algorithms have been proposed over the last few years. However, there is still wide room for improvement, especially in the development of more integrated and complete engine health management systems to detect, identify, and forecast complex faults in a short time. Furthermore, it is necessary to ensure that these systems preserve their capabilities over time despite engine deterioration. This paper addresses these necessities by proposing an integrated system that considers the joint operation of feature extraction, anomaly detection, fault identification, and prognostic algorithms for engines with long operation times. To effectively reveal the actual engine condition, light adaptive degraded engine models are computed along with different health indicators that are used as inputs to train and test recognition and prediction models. The system is developed and evaluated using a specialized NASA platform which provides data from a turbofan engine fleet simultaneously experiencing long-term performance deterioration and faults. Contrary to other compared solutions, our results show that the proposed system is robust against the effects of engine deterioration, maintaining its level of detection, recognition, and prediction accuracy over a total engine service life. The low computational cost algorithms has generally fast performance in all stages, making the system suitable for online applications.
Influence and Mechanism Study of Soil Moisture on the Stability of Arsenic-Bearing Ferrihydrite in Surface Soil Vertical Profiles
Authors: Li Lijuan , Chen Xinyi , Wang Yan , Zhang Fubin , Zhou Xinyi , Zhang Tuo
Published: March 2024

Ferrihydrite is usually used as a remedy for arsenic (As)-contaminated soil due to its strong affinity and large specific surface area. However, its noncrystalline phase makes it unstable in long-term applications in the soil. In this study, a soil incubation experiment was designed using the diffusive gradient in thin film (DGT) technique and spectral techniques to investigate the fate of As-bearing ferrihydrite [As(V)-Fh] after long-term incubation at different soil water holding capacities (SWHCs). After As(V)-Fh (0.05 and 0.005 As/Fe molar ratio) was incubated in soil for 360 days, both DGT-derived labile As and Fe were released at 70% SWHC and 120% SWHC into the soil (at a vertical depth of 12 cm). The concentrations of DGT-As and DGT-Fe increased with incubation time and were greater at 120% SWHC. The results of X-ray diffraction (XRD) and scanning electron microscopy (SEM) showed that As(V)-Fh gradually transformed into hematite and goethite after 360 days of incubation. Goethite was mainly found in the 120% SWHC treatment after 180 days. Hematite and goethite formation rates were greater in the 120% SWHC treatment and in the bottom soil layer. Mechanistic analysis based on X-ray photoelectron spectroscopy (XPS) revealed that the variation in soil pH and the formation of Fe(II) (under flooded water conditions) are the two key factors promoting the formation of hematite (dehydrogenation and dehydration) and goethite (As(V)-Fh dissociation and reorganization). The As release mainly occurred due to the loss of adsorption sites. Thus, it is recommended that ferrihydrite be applied in paddy–dry rotations or dry-field patterns to effectively avoid the loss of As(V)-Fh in long-term-saturated soil.
From Fields to Films: Exploring Starch from Agriculture Raw Materials for Biopolymers in Sustainable Food Packaging
Authors: Gonçalves Elsa M. , Silva Mafalda , Andrade Luiza , Pinheiro Joaquina
Published: March 2024

In the wake of escalating global concerns over the environmental impact of plastic pollution, there has been an unprecedented call for sustainable alternatives. The food-packaging industry, responsible for a staggering 40% of global plastic consumption, faces mounting challenges driven by environmental degradation and concerns about fossil fuel depletion. Motivated by these challenges, there is a growing interest in reducing reliance on traditional packaging and exploring eco-friendly solutions derived from renewable resources. Eco-efficient packaging, specifically derived from agricultural raw materials, emerges as a promising solution that aligns with ecological, economic, and social sustainability principles. Starch, abundant and versatile, emerges as a frontrunner among agricultural raw materials for biopolymers. Its inherent properties, including low cost, availability, biodegradability, and biocompatibility, make it a compelling choice. Starch-based bioplastics, with their potential to replace synthetic primary packaging materials, have gained traction due to their satisfactory mechanical and barrier properties. This review delves into the realm of starch-based films and coatings for food applications. It explores fundamental properties, advantages, and limitations, offering insights into potential improvements through various treatments or additive combinations. As technological advances drive the popularity of biodegradable starch-based packaging, this review aims to contribute to the ongoing discourse, providing a comprehensive overview and paving the way for more functional and widely applicable products in the ever-evolving landscape of sustainable packaging.
Design and Performance Test of 4UJ-180A Potato Picking and Bagging Machine
Authors: Yang Xu , Wu Yingsi , Wang Lihe , Liu Fei , Zhao Xuan , Bai Hongbin , Dong Wenxue , Kong Xiang , Hu Hengtong , Zhong Wendong , Xuan Dezheng , Yang Ao , Ma Yutao
Published: March 2024

To address problems encountered in current potato harvesting machines, such as potato damage, poor adaptability, and low operational efficiency, a new towed potato picking and bagging machine (4UJ-180A) equipped with a soil-digging axis, flexible conveying device, and hydraulic control system was developed. The digging mechanism of the harvester can reduce soil blockage and minimize damage to potato skins. The rubber biomimetic finger can maintain stable transportation of potatoes and minimize collisions. The hydraulic control system, the buffering components, and the bagging device work together to flexibly and continuously collect potatoes, reducing skin damage during the harvesting process. Based on the structure of the whole machine, the harvesting process, and the working principle, the soil picking, lifting buffer, and potato collection process were analyzed. Theoretical calculations were used to determine the structure and operational parameters of the potato picking, lifting buffering, and bagging segments. An experiment utilizing the orthogonal method was conducted. The experiment consisted of three factors and three levels, with the test indicators being the potato skin damage rate, potato injury rate, loss rate, and impurity rate. The factors considered in the experiment were the forward speed, conveyor speed, and soil-digging shaft speed. Field experiments demonstrate that at a forward speed of 1 m/s, soil digging shaft speed of 35 rpm, and conveyor speed of 28 rpm, the rate of potato skin damage is 2.8%, the potato injury rate is 1.3%, the loss rate is 0.4%, and the impurity rate is 0.7%. These experiments verify that all indicators adhere to national industry standards, providing a valuable reference for equipment research, development, optimization, and improvement.