bluuebunny/crossref_metadata_2025 huggingface dataset:
MedBe: Um Aplicativo Móvel para Agendamento Inovador de Consultas
Authors: Quincozes Vagner E. , Quincozes Silvio E. , Kreutz Diego , Mansilha Rodrigo B. , Kazienko Juliano F.
Published: September 2022

Este trabalho apresenta o aplicativo móvel MedBe, que conecta profissionais de saúde e bem estar (e.g., enfermagem, cuidador de idoso, psicologia, etc.) com pacientes que desejam agendar serviços. A principal inovação do MedBe é a possibilidade de agendamento de atendimentos sob demanda, através de um algoritmo de matching que aumenta a eficiência da busca para os pacientes, e amplia de maneira qualificativa o mercado para profissionais. Apresentamos as principais funcionalidades do aplicativo que está em operação e já conta com mais de 2.000 instalações e 350 solicitações. O público-alvo deste trabalho se beneficiará com insights de tecnologia e inovação no setor de saúde digital. Trabalhos futuros incluem a abordagem de aspectos de usabilidade, escalabilidade e implementação de técnicas de inteligência artificial.
PSECO-IM: An Approach for Incident Management to Support Governance in Proprietary Software Ecosystems
Authors: Costa Luiz Alexandre , Fontão Awdren , Santos Rodrigo
Published: September 2022

A proprietary software ecosystem (SECO) concerns data concentrated on a technological platform with contributions protected by intellectual property. The platform that supports the business initiatives is developed using different technologies with integration points, promoting a network of dependencies and architectural complexities. Systems downtime (incidents) causes major image and financial upheavals for organizations. To mitigate the risks of incidents, the IT management team should implement strategies based on governance mechanisms to sustain the platform. This work aims to develop and evaluate a process-based approach (PSECO-IM) for incident management to support the IT management team in governing a technology platform architecture in a proprietary SECO. This work is based on Work System Theory (WSF), in which the systems in organizations should be seen as work systems and technologies as components of work systems. As a final result, we got positive feedback on the relevance of our proposal from the organization’s practitioners, such as: i) mitigating the opening of incidents; ii) measuring the health of the proprietary SECO through new metrics and indicators; and iii) discovering patterns in software projects that may represent a chance of opening incidents. We argue that this work contributes to the Information Systems area since it covers studies on three pillars: people, process, and technology, such as tacit knowledge, low-quality software, governance, incident management, and proprietary SECO.
Potencialidades e Desafios do Ensino Remoto em um Curso de Bacharelado em Sistemas de Informação
Authors: Silveira Sidnei Renato , Vit Antonio Rodrigo Delepiane de , Bertolini Cristiano
Published: September 2022

Este artigo apresenta as impressões dos alunos de um curso de Bacharelado em Sistemas de Informação, sobre a modalidade de ensino remoto, adotada durante os anos de 2020 e 2021, em decorrência do isolamento social devido à Pandemia de COVID-19. As impressões foram coletadas durante a realização da disciplina de Computadores e Sociedade, no momento em que foram discutidas as potencialidades e os desafios da aplicação das Tecnologias Digitais da Informação e da Comunicação nos processos de ensino e de aprendizagem. Os resultados apontam potencialidades que favorecem o desenvolvimento do ensino remoto, híbrido e a distância, além de desafios que precisam ser superados.
Modelo de Simulação Urbana Baseada em Multiagente
Authors: Duarte Renan Procopio , Zamith Juliana M. N. , Silva Marcel , Zamith Marcelo
Published: September 2022

O crescimento rápido das cidades fez surgir alguns problemas sociais. Dentre esses problemas, destaca-se a segregação socioeconômica e espacial. Nesse sentido, a simulação computacional é uma excelente ferramenta para observar e entender dinâmicas sociais, como a segregação socioeconômica. Assim, este trabalho propõe um modelo de simulação baseado em multiagentes para simular a segregação socioeconômica e espacial desses grupos. Alêm disso, é também proposto no trabalho métricas capazes de medir o grau de segregação nas simulações, permitindo avaliar qualitativamente e quantitativamente os resultados.
Um Sistema Fuzzy para Redução do Consumo Energético e Manutenção da Qualidade de Serviço de Redes de Sensores Sem Fio
Authors: Oliveira Jaevillen F. , Pires Matheus G.
Published: September 2022

Este trabalho propõe o uso de Sistemas Baseados em Regras Fuzzy para controlar o funcionamento (ligado/desligado) dos pontos de acesso de Redes de Sensores Sem Fio, com objetivo de reduzir seu consumo energético, e ao mesmo tempo, mantendo a qualidade de serviço da rede, a qual foi medida pela taxa de perda de pacotes. Para demonstrar a eficiência da nossa proposta, ela foi comparada com outras três abordagens. De acordo com o resultados obtidos, foi possível demonstrar ganhos superiores, tanto na redução do consumo de energia quanto na redução da taxa de perda de pacotes, em relação às outras três.
Paradigma Orientado a Notificações para Aplicações de Internet das Coisas em Cidades Inteligentes
Authors: Figueiredo Luis H. P. , Simão Jean M. , Vendramin Ana Cristina B. Kochem
Published: September 2022

A Internet das Coisas para Cidades Inteligentes possibilita um melhor uso dos recursos e serviços. Porém, os desafios tecnológicos para a criação de sistemas computacionais eficientes aumentam devido ao número e complexidade das interações. Este artigo propõe distribuir as entidades do Paradigma Orientado a Notificações (PON) por meio de uma arquitetura publicação/assinatura com o protocolo MQTT. O desempenho do PON e do paradigma de programação orientado a objetos são comparados em uma aplicação genérica de Internet das Coisas. Os resultados mostram que ao distribuir as entidades do PON é possível reduzir o tempo de execução, processamento e resposta da aplicação ao custo de um maior uso de memória.
CityGuardian: Uma ferramenta para monitorar mudanças em padrões de criminalidade nas Cidades Inteligentes
Authors: Leal Manoel Flavio , Gomes-Jr Luiz
Published: September 2022

Para combater os altos índices de criminalidade, Cidades Inteligentes recorrem a ferramentas de monitoramento e suporte para subsidiar ações de prevenção. Analisar onde e quando um crime ocorreu é o primeiro passo para entender os padrões de criminalidade. Mudanças repentinas como a pandemia da covid-19, mostram a importância de entender um fato ocorrido para se tomar uma decisão assertiva. Dessa forma, apresentamos a CityGuardian, uma ferramenta web, que monitora as ocorrências criminais e alerta ao identificar alterações de padrões no espaço-tempo. Além disso permitindo explorar em sua interface gráfica os dados que causaram essas alterações.
Observatório de Projetos de Pesquisa e Extensão
Authors: Santos Júnior José Ferreira dos , F. Junior Ivaldir H. , Vieira Jeferson K. M.
Published: September 2022

O objetivo desta pesquisa foi conceber um observatório de projetos e extensão de uma universidade. Para alcançar este objetivo adotamos como metodologia uma pesquisa bibliográfica, onde extraímos as informações básicas, em seguida utilizamos a prototipação para desenvolver o observatório e por fim executamos uma survey com 25 pessoas visando avaliar o observatório em sua versão inicial. Como resultado esperado, percebemos que a avaliação do observatório foi positiva e que as partes interessadas anseiam para que o mesmo esteja pronto para ser colocado em produção.
Definitions, pathogenesis, and pharmacological options for bone marrow lesions: an updated review
Authors: Tarantino Umberto , Cariati Ida , Greggi Chiara , Bonanno Chiara , Romano Francesco , Iundusi Riccardo , Gasbarra Elena
Published: September 2022

The term “Bone Marrow Lesions” (BMLs) identifies a pathological state characterized by a structural degeneration of the osteochondral unit (OCU) and by an alteration of the biochemical balance existing between articular cartilage and subchondral bone. These lesions, if they do not resolve spontaneously and if not adequately treated, can give rise to chronic degenerative diseases such as osteoarthritis and, in the most serious cases, evolve into stress fractures. The technique considered to be the gold standard for the detection of BMLs is Magnetic Resonance Imaging (MRI), to which BMLs appear as an area of ill-defined hyperintensity (high signal) in subchondral bone in fat-suppressed T2-weighted sequences, and hypointense areas (low signal) in T1-weighted sequences. There are several pharmacological intervention strategies for the treatment of BMLs, primarily the administration of bisphosphonates, but in recent years Iloprost treatment is also proving to be an effective therapeutic strategy. The aim of this review is to provide further evidence on the sequence of clinical-biological events leading to the appearance of these lesions, and on the current treatment strategies with the best outcome, in order to shed light on the importance of conducting further research in this field, since BMLs are part of a pathological picture characterised by numerous variables.
Bone complications in Gaucher disease
Authors: Masi Laura
Published: September 2022

Purpose: Gaucher disease (GD; OMIM # 230800) is an autosomal recessively inherited lysosomal storage disease. GD is caused by a deficiency of the lysosomal enzyme, glucocerebrosidase (GBA, also called acid ß-glucosidase or GCase), which hydrolyzes glucosylceramide (GlcCer) into ceramide and glucose. As a consequence of mutations in the GBA1 gene located on chromosome 1 (1q21) there is an accumulation of GCase substrate, GlcCer, in macrophages. Bone tissue represents a large systemic compartment of the human body, with an active metabolism that controls mineral deposition and removal, and where several factors may play a role. For these reasons, several non-skeletal diseases may influence bone metabolism. Methods: The present review describes bone skeletal manifestations in the GD and the role of several factors. This manuscript is the result of a review of the literature that focused on the bone manifestations of GD. In particular, relevant studies were identified through a PubMed search strategy. Step 1 consisted of a systematic literature search using the terms: Bone Metabolic Rare Diseases, Hematological Rare Diseases, Gaucher Disease; step 2 involved adding the terms “osteoporosis” or “bone mass”, or “bone turnover” or “bone fragility” or “bone deformity”, or “bone biomarkers”. Results: The skeletal manifestations of GD include a variety of bone pathologies due to various factors. These pathologies include bone infarcts, avascular bone necrosis, cortical thinning, lytic bone lesions, osteosclerosis and fractures due to osteopenia or osteoporosis, and rarely acute osteomyelitis. Conclusions: Bone loss in patients with GD should be managed, whenever possible, at or in close liaison with a center that specializes in the diagnosis, management and therapy of metabolic bone diseases. A multidisciplinary approach is important to better understand the complexity and pathogenesis of bone involvement in GD. In this way it will be possible to refine and standardize the diagnostic and therapeutic approaches to bone disease in GD.
Leonardo Da Vinci unveiled degenerative diseases of the spine through his novel, exquisite anatomical drawings
Authors: Tonelli Francesco , Barbagli Matteo , Genant Harry , Brandi Maria Luisa
Published: September 2022

Leonardo da Vinci set out to produce a true treatise of anatomy, since in his time no such work existed. His drawing of the spine is a formidable example of his genius. Since artworks by Leonardo are appreciated for their accuracy and careful adherence to the subject represented, we examined Leonardo’s spine as one would a radiograph, looking for evidence of osteoporotic fractures or signs of osteoarthritis. The dorsal and lumbar vertebrae show a slightly wedge-shaped deformity without “radiological” signs of fracture. Instead, signs of osteoarthritis can be noted at the lumbar vertebrae. With his extraordinary eye for detail, Leonardo produced an image that foreshadows those that radiology would later make available to clinicians for the diagnosis of problems linked to vertebral degeneration.
“Hang in there mama!” The role of online parenting forums in maternal mental health
Authors: McSorley Lauren , Deighton-Smith Nova , Budds Kirsty , Wang Xu
Published: September 2022

The perinatal period can be challenging and yet reluctance to disclose wellbeing concerns to health professionals is well documented. The current study sought to explore how women use online parenting forums to seek advice and support for maternal mental health concerns. Forum threads (N = 153) from a popular UK parenting website were analysed to explore postpartum mothers’ discussions about their maternal mental health. Through reflexive thematic analysis, four themes were generated: (1) Navigating the motherhood role: unprepared and overwhelmed; (2) “I’m struggling, but looking on here I know I’m no way alone!”; (3) Challenging feelings of failure: “it doesn’t make you a bad mother”; and (4) Forums facilitate help seeking. There were two main findings; first, forums may act as a potential solution in offering a supportive, reassuring community and safe space for disclosure around maternal mental health concerns. Furthermore, forums are beneficial in providing women with an honest, realistic view and expectation of motherhood, thus supporting their transition to parenthood. Consequently, forums could be utilised to inform training for healthcare professionals to understand disclosure barriers.
No secrets between the two of us: Privacy concerns over using AI agents.
Authors: Lim Sohye , Shim Hongjin
Published: September 2022

The diverse spread of artificial intelligence (AI) agents provides evidence of the most notable changes in the current media landscape. AI agents mostly function based on voluntary and involuntary sharing of users’ personal information. Accordingly, users’ privacy concerns have become key to understanding the varied psychological responses towards AI agents. In this study, we adopt the “computers are social actors” paradigm to identify the association between a set of relational variables—intimacy, para-social interactions, and social presence—and privacy concerns and to determine whether a user’s motivations moderate this relationship. The results from an online survey (N = 562) revealed that this occurs primarily to gratify three AI agent user needs: entertainment motivation, instrumental motivation, and passing time. The results also confirmed that social presence and intimacy significantly influence users’ privacy concerns. These results support the moderating effect of both entertainment and instrumental motivation on the relationship between intimacy, para-social interaction, social presence, and privacy concerns about using AI agents. Further implications for privacy concerns in the context of AI-mediated communications are discussed.
The socially poor get richer, the rich get poorer: The effect of online self-disclosure on social connectedness and well-being is conditional on social anxiety and audience size
Authors: Desjarlais Malinda
Published: September 2022

Self-disclosure taking place in computer-mediated communication (CMC) is generally associated with enhanced well-being because it evokes a greater sense of connectedness. It has been established that the magnitude of the benefits reaped from online self-disclosure is conditional on social anxiety (under the lens of the poor-get-richer vs. rich-get-richer hypotheses) or audience size. What remains to be understood is whether those with low (compared to high) social anxiety experience greater social connectedness and subjective well-being in dyadic and/or masspersonal CMC. A sample of 411 Canadian undergraduate students (aged 17–21 years old) self-reported their anxiety in social situations, online self-disclosure in dyadic and masspersonal communication, current feelings of social connectedness, and subjective well-being. Model 7 of the PROCESS macro for SPSS was used to test the indirect effect of online self-disclosure on subjective well-being through feelings of social connectedness, conditioned on values of social anxiety. The model was run separately for dyadic and masspersonal CMC. Online self-disclosure was associated with positive outcomes only for those with high social anxiety. In both contexts, online self-disclosure was associated with enhanced social connectedness and in turn more positive subjective well-being. In contrast, for those with low social anxiety, increases in self-disclosure in masspersonal CMC was associated with decreases in social connectedness and poorer well-being. The indirect effect was not significant for dyadic CMC. Overall, the findings contribute to a more informed understanding of online self-disclosure as a double-edged sword. Theoretical implications for the poor-get-richer and rich-get-richer perspectives are discussed.
Is that Insta worthy? Predicting content sharing behavior on social media through interpersonal goals
Authors: Toh Zena , Lee David S
Published: September 2022

What motivates people to share contents that promote the image of the self versus the well-being of others on social media? Two studies examined how interpersonal goals, namely self-image goals and compassionate goals, influence intentions of sharing different contents on Instagram. According to research on interpersonal goals, self-image goals motivate people to maintain and demonstrate their desirable qualities to others; compassionate goals energize people to promote the well-being of others. Based on this research, we hypothesized that self-image goals (vs. compassionate goals) would propel people to have higher intentions to share contents that are self-promoting relatively more than contents that are prosocial. A correlational study that measured participants’ chronic (i.e., stable) interpersonal goals (Study 1; n = 126) and an experiment that manipulated participants’ incidental (i.e., momentary) interpersonal goals (Study 2; n = 201) confirmed these hypotheses. These results highlight the role of motivation in content sharing on social media and shed light on psychological mechanisms that help shape the social environment on Instagram.
Updated data on Litopterna from the Huayquerian Stage/Age (Late Miocene-Early Pliocene) of central-east Argentina
Authors: SCHMIDT Gabriela I. , MONTALVO Claudia I. , CERDEÑO Esperanza , SOSTILLO Renata , TOMASSINI Rodrigo L. , BONINI Ricardo A.
Published: September 2022

Hitherto, only the Proterotheriidae Ameghino, 1887 litopterns from the Cerro Azul Formation (Chasicoan-­Huayquerian Stages/Ages; Late Miocene-Early Pliocene) in La Pampa Province had been studied, recognizing Proterotheriidae indet. at Cerro La Bota, Diplasiotherium pampa Soria, 2001 at several localities,
A Multi-Level Approach for Flooding Risk Estimation and Assessment of Passenger Ships
Authors: Vassalos Dracos , Luhmann Henning , Cardinale Mike , Hamann Rainer , Papanikolaou Apostolos , Paterson Donald
Published: September 2022

Against the background of using the Index of Subdivision as a reference to address the safety level of ships when damaged, following primarily collision accidents, the EC-funded FLARE project is making inroads towards a direct assessment of the flooding risk, which is ship specific and considers the operating conditions and all accident-types leading to hull breach and flooding, namely collisions, bottom and side groundings. This is enabled by using a newly developed two-level approach Potential Loss of Life (PLL) of People On Board (POB); level 1 is a semi-empirical approach with the risk models formulated by use of data from a newly composed accident database, while level 2 is determining the flooding risk by a first principles approach, using time-domain flooding and evacuation analyses simulation tools in pertinent emergencies. Level 2 is considered in two sub-levels (Level 2.1 and Level 2.2), the former involving some simplifying assumptions in the risk calculation. In addition to addressing all accident types and modes of loss of ship and of POB, the FLARE framework and methodology considers active and passive measures of risk prevention and control, hence with application potential to both newbuildings and existing ships. It also facilitates real-time flooding risk evaluation for risk monitoring and effective control in emergencies. Key objectives of the FLARE project are to provide the technical basis for the rational determination and assessment of flooding risk and to prepare a proposal for the revision of relevant IMO SOLAS regulations towards a risk-based approach to contain and control flooding emergencies. The paper briefly describes the FLARE flooding risk assessment framework and provides risk estimation results at all three levels for one cruise ship and one RoPax, including comparisons with available IMO societal criteria, as well as a summary of results for a further 7 ships at levels 2.1 and 2.2. In the light of the results presented, the paper draws conclusions on the progress made and offers recommendations for the way forward.
Holistic Ship Design for Green Shipping
Authors: Papanikolaou Apostolos , Zaraphonitis George , Jokinen Markus , Aubert Adrien , Harries Stephan , Marzi Jochen , Mermiris George , Gunawan Rachmat
Published: September 2022

The pattern of seaborne trade and goods transportation is changing and ships need to adapt to changes of customer and market requirements, cargo volumes, and new legislation for the safety of ships and nowadays, even more, to the strict regulatory requirements for the protection of the environment. Responding to the urgent needs for substantial reduction of GHG (Green House gas) emissions from marine operations in line with the ambitious targets set by the International Maritime Organisation and the European Commission, a series of research and development works were initiated in the maritime sector for the ships designed and built today and be operating in the next decades to meet future environmental requirements. Responding to these needs, the recently completed Horizon 2020 European Research project – HOLISHIP – Holistic Optimisation of Ship Design and Operation for Life Cycle (2016-2020) has developed suitable tools and software platforms, as necessary for the creation of innovative design solutions meeting the set low emission strategic objectives. The present paper is presenting the HOLISHIP, multi-objective optimisation approach to green shipping and demonstrates a subset of its functionality by two green design RoPAX case studies.
Exploring a dialogic approach in tackling psychotic diseases: A systematic review
Authors: Fernández-Villardón Aitana , García-Carrión Rocío , Racionero-Plaza Sandra
Published: September 2022

Psychosis is a severe mental disorder traditionally treated from a purely biological approach, which often has led to a dehumanisation in psychiatric hospitals. To counteract this trend and aiming at tackling the disease with a more human approach, psychosocial interventions have increased. In this vein, interventions based on a dialogic approach, with specific features such as egalitarian dialogue, foster psychological and social transformations in different contexts. This review aims to analyse the clinical and social effects of psychological interventions based on these principles when treating patients with psychosis. A systematic review has been carried out, following PRISMA recommendations, in the Web of Science, Scopus and PsycInfo databases. After applying the inclusion criteria, thirteen empirical studies were selected and analysed, including a quality assessment. These studies show that when introducing a dialogic approach in treating psychosis, positive clinical and relational outcomes are obtained. Likewise, patients value positively these collaborative environments, where they feel listened and understood. Whereas dialogism emerges as a tool with potential benefits to tackle psychosis, more studies are needed to clearer determine the influence of specific dialogic features on improving psychotic patients’ mental health.

La psicosis es un trastorno mental grave que tradicionalmente se ha tratado desde un enfoque puramente biológico, lo que a menudo ha conllevado una deshumanización en los hospitales psiquiátricos. Con el fin de contrarrestar esta tendencia y con el objetivo de abordar la enfermedad desde enfoque más humano, las intervenciones psicosociales han aumentado. En esta línea, las intervenciones basadas en un enfoque dialógico, con características específicas como el diálogo igualitario, fomentan transformaciones psicológicas y sociales en diferentes contextos. Esta revisión pretende analizar el impacto clínico y social de las intervenciones psicológicas basadas en principios dialógicos en pacientes con psicosis. Se ha realizado una revisión sistemática, siguiendo las recomendaciones PRISMA, en las bases de datos Web of Science, Scopus y PsycInfo. Tras aplicar los criterios de inclusión, se seleccionaron y analizaron trece estudios empíricos, además, se ha incluido una evaluación de la calidad de dichos estudios. Estos estudios muestran que, al incorporar un enfoque dialógico en el tratamiento de la psicosis, se obtienen resultados clínicos y relacionales positivos. De la misma manera, los pacientes valoran positivamente estos entornos de colaboración, donde se sienten escuchados y comprendidos. Aunque el dialogismo emerge como una herramienta eficaz para abordar la psicosis, se necesitan más estudios para determinar con mayor claridad la influencia de características dialógicas específicas en la salud mental de los pacientes.
Coloración de Ziehl-Neelsen en el laboratorio de patología: rendimiento y contribución al diagnóstico de micobacterias en el lavado broncoalveolar
Authors: Arteta Ariel A. , Arias Luis F. , Cadavid Claudia E.
Published: September 2022

Introducción. La coloración de Ziehl-Neelsen, con más de 100 años de uso, continúa vigente mundialmente.Objetivo. Comparar el rendimiento de las pruebas diagnósticas utilizadas para la determinación de micobacterias en el laboratorio clínico de patología en muestras de lavado broncoalveolar.Materiales y métodos. Se revisaron retrospectivamente 737 muestras de lavado broncoalveolar procesadas en el 2019 y el 2020 en el Hospital San Vicente Fundación (Medellín, Colombia) y se compararon las características de tres pruebas diagnósticas realizadas en paralelo: la reacción en cadena de la polimerasa (PCR) para micobacterias con detección de resistencia, el cultivo, y la coloración de Ziehl-Neelsen.Resultados. Se catalogaron como enfermos a 93 de los 737 pacientes a partir de los resultados positivos en alguna de las tres pruebas. El cultivo tuvo una sensibilidad de 0,80, la PCR una de 0,76 y la coloración de Ziehl-Neelsen una de 0,51. Sin embargo, solo 5 de 75 (6,5 %) cultivos fueron positivos a las cuatro semanas y el resto lo fue a las ocho semanas. La PCR combinada con la coloración de Ziehl-Neelsen mejoró la sensibilidad de la PCR por sí sola, de 0,76 a 0,88, diferencia que fue estadísticamente significativa (p=0,022).Conclusión. En las muestras de lavado broncoalveolar, el cultivo sigue siendo la prueba con mejor sensibilidad. El uso conjunto de la prueba de PCR y la coloración de Ziehl-Neelsen mejora significativamente la sensibilidad de la primera, lo que compensa la demora relativa en la entrega de los resultados debida al tiempo requerido para la tinción de Ziehl-Neelsen.
Revisão sistemática da produção científica brasileira sobre turismo e tecnologia da informação e comunicação (TIC)
Authors: Soares Ralyson Adyson Marques da Costa , Albuquerque Thyago Velozo de , Mendes-Filho Luiz , Alexandre Mauro Lemuel
Published: September 2022

O rápido desenvolvimento das tecnologias da informação e comunicação (TICs) foi fundamental para o progresso do turismo, sendo considerado ainda como um dos elementos principais para a competitividade da atividade. O objetivo deste estudo é traçar um panorama atual das publicações de turismo e TICs nas revistas científicas de Turismo brasileiras, a fim de identificar as principais temáticas estudadas e as perspectivas futuras para pesquisas neste campo. Como estratégia metodológica, optou-se pela revisão sistemática da literatura, definindo como fonte de dados periódicos nacionais na área do Turismo. Os 292 artigos que compuseram o universo da pesquisa foram categorizados de acordo com a classificação de Law, Leung e Chan (2019), e analisados por meio da análise de conteúdo apoiada no software Iramuteq. Como resultado pode-se perceber uma evolução crescente nos estudos desta temática, principalmente a partir de 2014. A classificação das análises identificou que 54% concentraram-se na perspectiva dos Fornecedores, enquanto 46% estão sob a ótica dos Consumidores. Este crescimento e qualidade das pesquisas reforçam a relevância da temática nos estudos e refletem a importância que as TICs vêm tomando na prática na atividade turística.
Assessment of Progress of Kisan Credit Card Scheme in Haryana, India
Authors: Sonia . , Malik D. P. , Sanjay .
Published: September 2022

Kisan Credit Card scheme is a very important institutional credit source in India. The study was conducted with time series data from the year 1999 to 2019 in order to work out the growth patterns in the number of Kisan Credit Cards (KCCs) issued and the amount sanctioned under KCC. Compound annual growth rate and coefficient of variation were calculated in order to better understand fluctuations in the number of KCCs issued and the amount sanctioned year by year. This research assessed the progress of the Kisan Credit Card scheme in Haryana.  Also, the amount sanctioned under the Kisan Credit Card scheme was found progressive during 1999-2019. The number of cards issued in Haryana attributed to a growth rate of more than 3.05 percent per annum and the amount sanctioned under the KCC scheme accounted for a growth rate of 8.2 percent annually. In Haryana among the various agencies, commercial banks, and RRBs showed a positive growth rate while co-operative banks exhibited a negative growth rate during 1999-2019. The annual growth rates for a number of Kisan Credit Cards issued by commercial banks, regional rural banks, and co-operative banks were 9.6, 12.26, and -10.38 percent, respectively. The growth rates for the amount sanctioned under the KCC scheme were found to be 16, 12.26, and -13.8 percent annum. Progress of KCC was found quite uniform in selected districts of three zones of Haryana during 1999-2019. The compound growth rates for the number of cards issued were found to be 20.73, 18.82, and 18.67 percent in Karnal, Hisar, and Jhajjar districts, respectively. The year-wise, agency-wise, and zone-wise progress for the number of KCCs issued in the state has been quite impressive which is near to average of all of India.
Structural Characteristics of Lightweight Ferrocement Beams with Different Core Material
Authors: Shaheen Yousry B. I. , El-Sabbwy Mohamed , El-Bordeny Dina Ahmed Hosny
Published: September 2022

The main objective of this research is to investigate experimentally the viability and effectiveness of the proposed ferrocement permanent forms and the effect of the test parameters on the behavior of the reinforced concrete ferrocement beams. And investigate the viability of using lightweight materials for the core with the aim of producing light weight structural system. The experimental program comprised casting and testing of eleven reinforced concrete beams of dimensions 200x100x2000mm.These beams are organized in four groups. Group one is the control group in which beams are cast using ordinary formwork and ferrocement. The beams in this group were reinforced with two steel bars of 10 mm at the top and12mm bottom of the beams.  The beams in the other three groups were cast using Ferrocement beams. The core of these beams was reinforced with two steel bars of 10 mm diameter at the top and12 mm bottom of the beam. Two types of steel mesh used to reinforce the ferrocement beams namely expanded steel mesh, and welded wires mesh. Single mesh and double layers consisting of mesh together with a strip steel mesh were studied. Three types of core material were investigated:  light brick, foam, and light weight concrete admixture with perlite. The test specimens were cast and tested until they failed under flexure load. The performance of the test beams in terms of strength, cracking behavior, ductility, and energy absorption properties was investigated. The use double layer of expanded steel mesh as the additional reinforcement of the main steel can achieved the best performance of the Light weight of ferrocement beams. The results of this research showed that ferrocement beams of light weight cores may be promising as an alternative to conventional beams and may be viable alternatives especially for low-cost residential buildings.
Characterization of the calcium‐binding sites of calcineurin B
Authors: Kakalis Lazaros T , Kennedy Michael , Sikkink Robert , Rusnak Frank , Armitage Ian M
Published: July 2002

Calcineurin (CaN) is a calcium‐ and calmodulin‐dependent serine/threonine phosphatase whose inhibition by the immunosuppressant‐immunophilin complexes (cyclosporin‐cyclophilin and FK506‐FKBP) is considered key to the mechanism of immunosuppression. CaN is a heterodimer, consisting of a 59 kDa catalytic subunit (A) and a 19 kDa calcium‐binding regulatory subunit (B). The latter is postulated to harbor four calcium binding domains of the EF hand type. The titration of the CaN B apoprotein with the isomorphic Cd2+ was followed by 113Cd NMR and these data support one high‐affinity metal binding site and three lower‐affinity ones. Flow dialysis data with Ca2+ indicate one high affinity calcium binding site with K
d ∼ 2.4 × 10−8 M and three other sites with K
d ∼ 1.5 × 10−5 M. The chemical shifts of all four 113Cd resonances (−75, −93, −106 and −119 ppm) are in the same range as found in other 113Cd substituted calcium‐binding proteins, and are indicative of all‐oxygen coordination of pentagonal bipyramidal geometry.
Three of four pseudoknots in tmRNA are interchangeable and are substitutable with single‐stranded RNAs
Authors: Nameki Nobukazu , Tadaki Toshimasa , Himeno Hyouta , Muto Akira
Published: July 2002

A novel translation, trans‐translation, is facilitated by a highly structured RNA molecule, tmRNA. This molecule has two structural domains, a tRNA domain and an mRNA domain, the latter including four pseudoknot structures (PK1 to PK4). Here, we show that replacement of each of these pseudoknots, except PK1, in Escherichia coli tmRNA with a single stranded RNA did not seriously affect the functions as an alanine tRNA and as an mRNA. Furthermore, these three pseudoknots were interchangeable with only small losses of the two functions. These findings suggest that neither PK2, PK3 nor PK4 interacts in a functional manner with ribosome during the trans‐translation process. Together with an earlier study showing the significance of PK1, it is concluded that among the four pseudoknots, PK1 is the most functional.
Melatonin receptor pharmacology: toward subtype specificity
Authors: Sugden David , Pickering Hannah , Teh Mui‐Teck , Garratt Peter J.
Published: July 2002

The recent cloning of three distinct melatonin receptor subtypes (Mel1a, Mel1b and Mel1c) which are part of a new family of G‐protein coupled receptors, and probably mediate the physiological actions of the hormone, has spurred interest in the design of analogues with subtype selectivity. The 5‐methoxyl and N‐acetyl groups of melatonin are important for binding to and activation of the receptor. The indole nucleus serves to hold these two groups at the correct distance from one another and allows them to adopt the required orientation for interaction with the receptor binding pocket. We have investigated the subtype selectivity of a number of analogues of melatonin in which the structure has systematically been modified in order to probe the similarities and differences in the interaction of ligand and receptor subtype. At all three subtypes 5‐methoxyl and N‐acetyl groups of melatonin are important for high affinity binding. However, replacing the 5‐methoxyl group (eg with 5‐H, 5‐OH, 5‐Me or 5‐BzO) reduces affinity much less at the Mel1b receptor subtype than at either Mel1a or Mel1c cloned subtypes. This suggests differences between the Mel1b and Mel 1a/1c subtypes in the size and shape of the binding pocket or in the manner in which melatonin interacts with the receptor at this position. Further studies have revealed that analogues with longer N‐acyl carbon chains behave similarly at each subtype. These observations suggest that the ‘pocket’ into which the N‐acetyl group fits is very similar for each subtype. Substitutions at the 2‐position on the indole ring improved affinity at each receptor subtype but did not give selective analogues. The systematic ‘mapping’ of the requirements for binding at each receptor subtype should allow the design of more selective agonists and antagonists, which will be valuable tools for the characterization and classification of functional melatonin receptors.
Analysis of rates of receptor‐mediated endocytosis and exocytosis of a fluorescent hapten‐protein conjugate in murine macrophage: Implications for antigen processing
Authors: Weaver Donald J. , Voss Edward W.
Published: July 2002

A novel fluorescent hapten‐protein conjugate was constructed to monitor the events required for CD 4+ T lymphocyte recognition of antigenic proteins. Previous studies utilizing the probe demonstrated that the hapten‐protein was localized to an acidic endocytic compartment within the macrophage and that the hapten‐protein was sensitive to multiple intracellular events including enzymatic degradation, acidification, and disulfide bond reduction. More importantly, recent experiments indicated that efficient internalization of the probe was dependent upon specific recognition of the hapten. Therefore, the present report addressed the effect of receptor‐mediated endocytosis upon the processing of the hapten‐protein within murine peritoneal macrophage. These studies determined that the rate of endocytosis was significantly faster than the rate of exocytosis. Specifically, the rate of exocytosis was estimated to be 3.4 × 104 s−1 based on a unimolecular rate constant. Although at higher concentrations, a slightly slower rate was observed (1.9 × 104 s−1). This study also represented one of the first efforts to measure the intracellular concentration effect typically associated with receptor‐mediated endocytosis. Experiments involving a radioactively labeled hapten‐protein conjugate revealed that the probe was at 100‐fold higher concentration within the endocytic vesicles when compared to the extracellular media. The intracellular mechanism involved in this phenomenon was discussed as well as the implications of these findings upon MHC II‐peptide binding.
Chromate Reduction in Shewanellaoneidensis MR‐1 Is an Inducible Process Associated with Anaerobic Growth
Authors: Viamajala Sridhar , Peyton Brent M. , Apel William A. , Petersen James N.
Published: July 2002

Cr(VI) reduction was observed during tests with Shewanella oneidensis MR‐1 (previously named S. putrefaciens MR‐1) while being grown with nitrate or fumarate as electron acceptor and lactate as electron donor. From the onset of anoxic growth on fumarate, we measured a gradual and progressive increase in the specific Cr(VI) reduction rate with incubation time until a maximum was reached at late exponential/early stationary phase. Under denitrifying conditions, the specific Cr(VI) reduction rate was inhibited by nitrite, which is produced during nitrate reduction. However, once nitrite was consumed, the specific reduction rate increased until a maximum was reached, again during the late exponential/early stationary phase. Thus, under both fumarate‐ and nitrate‐reducing conditions, an increase in the specific Cr(VI) reduction rate was observed as the microorganisms transition from oxic to anoxic growth conditions, presumably as a result of induction of enzyme systems capable of reducing Cr(VI). Although Cr(VI) reduction has been studied in MR‐1 and in other facultative bacteria under both oxic and anoxic conditions, a transition in specific reduction rates based on physiological conditions during growth is a novel finding. Such physiological responses provide information required for optimizing the operation of in situ systems for remediating groundwater contaminated with heavy metals and radionuclides, especially those that are characterized by temporal variations in oxygen content. Moreover, such information may point the way to a better understanding of the cellular processes used by soil bacteria to accomplish Cr(VI) reduction.
Field Gradient Focusing: A Novel Method for Protein Separation
Authors: Koegler Wendy S. , Ivory Cornelius F.
Published: July 2002

Equilibrium gradient techniques constitute a class of separation methods that combine the steps of separation and concentration by using a gradient in one or more counteracting forces to create a stable equilibrium point at which a protein can focus. Different proteins focus at different equilibrium points, creating a steady‐state distribution of isolated proteins. Equilibrium gradient techniques can be adapted to a specific separation by choosing appropriate counteracting forces based on differences in the physical properties of the proteins involved. Zone electric field gradient focusing (FGF) is a new addition to this class of separation techniques with the unique property of using a gradient in the electric field to establish an equilibrium point instead of using a gradient in the velocity or pH. This paper presents two mathematical models which can be used to predict the steady‐state concentration profiles obtained by zone electric field gradient focusing. The first model applies only at very low protein concentrations where nonlinear effects can be ignored, e.g., less than 1 mg/mL, but it can be solved analytically and is useful in understanding the basic principles engendered in the method. The second model applies at all concentrations and allows for variations in the electric field strength where the protein focuses, but requires numerical solution. The design of an experimental device is also reported, as well as the results of two experiments: (1) the focusing of the protein hemoglobin from a dilute solution and (2) the separation of different oxidation states of the protein myoglobin.
Solid State Fermentation of Lignocellulosic Waste for Cellulase and β‐Glucosidase Production by Cocultivation ofAspergillus ellipticusand Aspergillus fumigatus
Authors: Gupte Akshaya , Madamwar Datta
Published: July 2002

Lignocellulosic wastes available in abundance can be excellent substrates for the production of cellulase and β‐glucosidase enzymes by solid state fermentation (SSF) . A cellulase system, showing enhanced hydrolytic potential and β‐glucosidase under SSF, was obtained by cocultivation of Aspergillus ellipticus and Aspergillus fumigatus. Different types of substrates and various pretreatments were used to improve hydrolytic process. Among the various substrates examined, sugarcane bagasse gave the highest activity and 2% calcium hydroxide treatment was found to be the most favorable treatment for the enzyme production. The maximum enzyme production was obtained on the eighth day of the fermentation process.
Inverse Flux Analysis for Reduction of Acetate Excretion in Escherichia coli
Authors: Delgado Javier , Liao James C.
Published: July 2002

The determination of intracellular fluxes from the measurement of extracellular rates, a technique known as flux analysis, has been used successfully on several systems important to biotechnology. However, calculation of intracellular fluxes does not directly suggest ways to improve the performance of the cell. Here, we introduce a variation of this analysis, termed inverse flux analysis, which allows the prediction of the flux distribution as a function of manipulable internal fluxes. This approach is applied to analyze the acetate excretion problem commonly observed in aerobic Escherichia coli cultures. The effect of manipulating each flux is quantified by a flux distribution sensitivity coefficient, which is dependent on the stoichiometry. For the acetate excretion problem, our results suggest that the anaplerotic pathways, including the reactions catalyzed by phosphoenolpyruvate carboxylase and the glyoxylate bypass are the best choices for manipulating acetate excretion in E.coli. Increasing the anaplerotic flux will decrease acetate production while increasing the growth yield.
Mitochondria-dependent and -independent mechanisms in tumour necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis are both regulated by interferon-γ in human breast tumour cells
Authors: RUIZ-RUIZ Carmen , LÓPEZ-RIVAS Abelardo
Published: July 2002

Tumour necrosis factor-related apoptosis-inducing ligand (TRAIL/APO-2L) induces apoptosis in a variety of tumour cells upon binding to death receptors TRAIL-R1 and TRAIL-R2. Here we describe the sensitization by interferon (IFN)-γ to TRAIL-induced apoptosis in the breast tumour cell lines MCF-7 and MDA-MB231. IFN-γ promoted TRAIL-mediated activation of caspase-8, Bcl-2 interacting domain death agonist (Bid) degradation, Bcl-2-associated X protein (Bax) translocation to mitochondria, cytochrome c release to the cytosol and activation of caspase-9 in these cell lines. No changes in the expression of TRAIL receptors were observed upon IFN-γ treatment. Overexpression of Bcl-2 in MCF-7 cells completely inhibited IFN-γ-induced sensitization to TRAIL-mediated cell death. Interestingly, TRAIL-induced apoptosis was also clearly enhanced by IFN-γ in caspase-3-overexpressing MCF-7 cells, in the absence of Bax translocation to mitochondria and cytochrome c release to the cytosol. In summary, our results suggest that IFN-γ facilitates TRAIL-induced activation of mitochondria-regulated as well as mitochondria-independent apoptotic pathways in breast tumour cells.