bluuebunny/crossref_metadata_2025 huggingface dataset:
A Systematic Review of Studies Describing the Effectiveness, Acceptability, and Potential Harms of Place-Based Interventions to Address Loneliness and Mental Health Problems
Authors: Hsueh Yung-Chia , Batchelor Rachel , Liebmann Margaux , Dhanani Ashley , Vaughan Laura , Fett Anne-Kathrin , Mann Farhana , Pitman Alexandra
Published: April 2022

Given the links between the built environment and loneliness, there is interest in using place-based approaches (addressing built environment characteristics and related socio-spatial factors) in local communities to tackle loneliness and mental health problems. However, few studies have described the effectiveness, acceptability, or potential harms of such interventions. This review aimed to synthesize the literature describing local community-based interventions that target place-based factors to address loneliness and mental health problems, informing the development of future public health approaches. We searched PsycINFO, Medline, and Embase using a structured search strategy to identify English-language studies evaluating the effectiveness, acceptability, and potential harms of place-based community interventions in addressing loneliness and mental health problems, both in general and clinical populations. Seven studies met the inclusion criteria, classified as evaluating provision of community facilities (such as clubhouses), active engagement in local green spaces, and housing regeneration. None were randomised trials. Quantitative and qualitative findings suggested promising effects and/or acceptability of six interventions, with minimal potential harms. There is a clear need for randomised trials or quasi-experimental studies of place-based interventions to describe their effectiveness in addressing loneliness and mental health problems, as well as complementary qualitative work investigating acceptability. This will inform future policy development.
Self-Selected Walking Cadence after 16-Week Light-Intensity Physical Activity Intervention for Older Cancer Survivors
Authors: Harding Elizabeth M. , Gibson Ann L. , Kang Huining , Zuhl Micah N. , Sharma Harsh , Blair Cindy K.
Published: April 2022

In this secondary analysis of a light-intensity physical activity intervention, we hypothesized that older cancer survivors would self-select a faster walking cadence to meet their daily step goals. Average steps/day and free-living walking cadence were measured in 41 participants (age 69 ± 3.1 years) with an ActivPAL monitor worn 7 days pre- and post-intervention. Besides peak and average walking cadence, stepping patterns associated with ambulatory intensity were sorted in cadence bands of 20 steps/min from 40–59 (incidental movement) to ≥120 steps/min (fast locomotor movement). Compared to the waitlist Control group (n = 17), the Intervention group (n = 24) increased their peak 30-min cadence (4.3 vs. 1.9 steps/minute; p = 0.03), average 10-min cadence (4.1 vs. −6.6 steps/minute; p = 0.04), and average 30-min cadence (5.7 vs. −0.8 steps/minute, p = 0.03). Steps taken in cadence bands denoting moderate-intensity physical activity (100–119 steps/min) increased by 478 (interquartile range (IQR): −121 to 1844) compared to decreasing by 92 (IQR: −510 to 181) steps/day for the intervention and Control groups, respectively (p < 0.01). Evaluation of free-living walking cadence and patterns of ambulatory behavior can inform future interventions targeting behavior change, especially in those populations most at risk for reduced physical activity and vulnerable to mobility deficits and loss of independence.
Representations of Sexuality among Persons with Intellectual Disability, as Perceived by Professionals in Specialized Institutions: A Systematic Review
Authors: Guenoun Tamara , Smaniotto Barbara , Clesse Christophe , Mauran-Mignorat Marion , Veyron-Lacroix Estelle , Ciccone Albert , Essadek Aziz
Published: April 2022

The objective of this systematic review is to make an inventory of the representations of the professionals of specialized institutions on the sexuality of persons with intellectual disabilities. The scientific studies were identified according to the PRISMA protocol using 18 databases, with keywords on sexuality and parenthood. Studies were reviewed through a methodological assessment and then a thematic analysis. Twenty-four studies were reviewed and three themes were identified: professionals’ representations of gender, sexuality, and consent; professionals’ perceptions of their role in supporting people’s sexual lives; and the ways in which professionals construct representations of people’s sexual lives. This corpus highlights deep paradoxes in the representations of professionals concerning the socio-affective needs and sexuality of people with intellectual disabilities, creating what we could define as a “system of incompatibility” and leading to difficulties in positioning. Support is still too random and subject to control logics in the name of protecting users, who are perceived as vulnerable. Training and new ways of teamwork appear to be central to supporting the evolution of the representations and practices of professionals. Future research anchored in practices and involving users as well as professionals is necessary to better understand the paradoxical aspects of professionals’ representations and to draw alternative ways of constructing these representations.
Development of a Framework for Scaling Up Community-Based Health Promotion: A Best Fit Framework Synthesis
Authors: Weber Philipp , Birkholz Leonie , Kohler Simone , Helsper Natalie , Dippon Lea , Ruetten Alfred , Pfeifer Klaus , Semrau Jana
Published: April 2022

Community-based health promotion with a focus on people with social disadvantages is essential to address persistently existing health inequities. However, achieving an impact on public health requires scaling up such approaches beyond manifold funded pilot projects. The aim of this qualitative review is to provide an overview of scaling-up frameworks in health promotion and to identify key components for scaling up community-based health promotion. First, we conducted a systematic search for scaling-up frameworks for health promotion in PubMed, CINAHL, Scopus, Web of Science, PsycInfo, and SportDiscus. Based on the included frameworks, we created an a priori framework. Second, we searched for primary research studies in the same databases that reported scaling-up processes of community-based health promotion. We coded the data using the a priori framework. From 80 articles, a total of 12 frameworks were eligible, and 5 were included for data extraction. The analysis yielded 10 a priori defined key components: “innovation characteristics”; “clarify and coordinate roles and responsibilities”; “build up skills, knowledge, and capacity”; “mobilize and sustain resources”; “initiate and maintain regular communication”; “plan, conduct, and apply assessment, monitoring, and evaluation”; “develop political commitment and advocacy”; “build and foster collaboration”; “encourage participation and ownership”; and “plan and follow strategic approaches”. We further identified 113 primary research studies; 10 were eligible. No new key components were found, but all a priori defined key components were supported by the studies. Ten key components for scaling up community-based health promotion represent the final framework. We further identified “encourage participation and ownership” as a crucial component regarding health equity.
Electrochemical Oxidation of Methyl Orange in an Active Carbon Packed Electrode Reactor (ACPER): Degradation Performance and Kinetic Simulation
Authors: Hou Jing , Li Xue , Yan Yuting , Wang Lizhang
Published: April 2022

The efficient removal and kinetic modelling of methyl orange (MO) degradation using an electrocatalytic oxidation method in an activated carbon (AC) packed electrode reactor (ACPER) were conducted. A significantly high (81.2%) chemical oxygen demand (COD) and 100.0% MO decolorization efficiency were observed under the experimental conditions of current density of 3.0 mA·cm−2, flow velocity of 0.3 L·h−1, and treatment duration of 1.68 h using a β-PbO2/Ti anode. The high removal efficiency is ascribed to the anode expansion effect after AC packing. The anode expansion coefficient (λ) of the ACPER was calculated to be 0.63 from the cyclic voltammetry (CV) measurement, which means the further current utilization for MO oxidation. Based on the current utilization efficiency on anodic and particle electrode surfaces, a phase-reaction kinetics model was proposed for the simulation of MO COD removal efficiency. Our simulation results showed that the newly established average current efficiency (ACE) and energy consumption (Esp) model well matched the MO experimental degradation data. Our work broadens the scope of the application of ACPER in the treatment industry wastewater containing organics and provides a new strategy for the energy utilization evaluation during the removal of organic matter by electrocatalytic oxidation.
Implementation of a Psychiatric Consultation for Healthcare Workers during First Wave of COVID-19 Outbreak
Authors: Benzakour Lamyae , Langlois Gérard , Marini Verena , Groz Alexandra , Chiabotto Chiara , Apetrei Diana , Corneau Bruno , Bondolfi Guido
Published: April 2022

Background: Prevention and management strategies of mental suffering in healthcare workers appeared as important challenges during the COVID-19 pandemic. This article aims to: (1) show how potential psychiatric disorders for healthcare workers (HCW) during the first wave of the COVID-19 outbreak were identified; (2) present an activity report of this consultation; and (3) analyze and learn from this experience for the future. Methods: We performed a retrospective quantitative analysis of socio-demographic and clinical data, in addition to psychiatric scales scores for the main potential psychiatric risks (PDI, PDEQ, PCL-5, HADS, MBI-HSS) and post-hoc qualitative analysis of written interviews. Results: Twenty-five healthcare workers consulted between 19 March 2020 and 12 June 2020. We found 78.57% presented high peritraumatic dissociation and peritraumatic distress, 68.75% had severe anxiety symptoms, and 31.25% had severe depression symptoms. Concerning burnout, we found that 23.53% had a high level of emotional exhaustion. In the qualitative analysis of the written interview, we found a direct link between stress and the COVID-19 pandemic, primarily concerning traumatic stressors, and secondarily with work-related stress. Conclusions: Early detection of traumatic reactions, valorization of individual effort, and limitations on work overload appear like potential key preventive measures to prevent psychiatric complications for healthcare workers in the context of the COVID-19 pandemic.
Biofilm and Gene Expression Characteristics of the Carbapenem-Resistant Enterobacterales, Escherichia coli IMP, and Klebsiella pneumoniae NDM-1 Associated with Common Bacterial Infections
Authors: Al-Bayati Majid , Samarasinghe Shivanthi
Published: April 2022

In light of the limited therapeutic options with Carbapenem-Resistant Enterobacterales (CRE) infections, understanding the bacterial risk factors, such as biofilm formation and related gene expression of CRE, is vital. This study investigates the biofilm formation and biofilm-related gene expression of two enteric Enterobacterales with major CR determinants Escherichia coli IMP and Klebsiella pneumoniae NDM-1, which were seen in high prevalence in most common bacterial infections over the past few years. To our knowledge, this is the first study that demonstrated the relationship between biofilm formation and the related gene expression, to understand the potential molecular mechanisms during the biofilm formation in CRE. Biofilms were quantified by tissue culture plate assay at the stages of the biofilm development: initial attachment (6 h), microcolony formation (12 h), maturation (24 h), and dispersion (48 h). In a dispersion, event bacteria detach without any mechanical means and colonise another area. To investigate the influence of different growth conditions on biofilm formation, biofilms were quantified under different growth conditions. In parallel, quantitative real-time PCR (qPCR) assessed the biofilm-related gene expression of a cluster of genes, including biofilm maturation, quorum sensing, stress survival, and antibiotic resistance. Structural changes during biofilm development were assessed via confocal laser scanning microscopy (CLSM). We observed that the biofilm formation of CRE is correlated with the biofilm development stages, with maximum biofilm observed at 24 h at the maturation stage. Our data also showed that biofilm growth, under the condition tested, is the major factor influencing the variability of biofilm gene expression quantification assays. qPCR analyses have demonstrated that the expression of biofilm-related genes is highly correlated with phenotypic biofilm development, and these findings can be further expanded to understand the variation in regulation of such genes in these significant CRE pathogens. Our study demonstrated that both CRE strains, E. coli IMP and K. pneumoniae NDM-1, are high biofilm formers, and genes involved in biofilm development are upregulated during biofilm growth. The characteristic of the increased biofilm formation with the upregulation of antibiotic-resistant and biofilm-related genes indicates the successful pathogenic role of biofilms of these selected CRE and is attributed to their multi-drug resistance ability and successful dissemination of CRE in common bacterial infections.
An Adaptive Embedding Network with Spatial Constraints for the Use of Few-Shot Learning in Endangered-Animal Detection
Authors: Feng Jiangfan , Li Juncai
Published: April 2022

Image recording is now ubiquitous in the fields of endangered-animal conservation and GIS. However, endangered animals are rarely seen, and, thus, only a few samples of images of them are available. In particular, the study of endangered-animal detection has a vital spatial component. We propose an adaptive, few-shot learning approach to endangered-animal detection through data augmentation by applying constraints on the mixture of foreground and background images based on species distributions. First, the pre-trained, salient network U2-Net segments the foregrounds and backgrounds of images of endangered animals. Then, the pre-trained image completion network CR-Fill is used to repair the incomplete environment. Furthermore, our approach identifies a foreground–background mixture of different images to produce multiple new image examples, using the relation network to permit a more realistic mixture of foreground and background images. It does not require further supervision, and it is easy to embed into existing networks, which learn to compensate for the uncertainties and nonstationarities of few-shot learning. Our experimental results are in excellent agreement with theoretical predictions by different evaluation metrics, and they unveil the future potential of video surveillance to address endangered-animal detection in studies of their behavior and conservation.
Novel Molecular Approaches to the Understanding of Pathophysiologal Mechanisms Acting in Human Embryos: An Editorial
Authors: Kovacs Gabor L. , Gombos Katalin
Published: April 2022

Infertility is a rapidly evolving global health problem [...]
Pharmacological Effects of Panduratin A on Renal Cyst Development in In Vitro and In Vivo Models of Polycystic Kidney Disease
Authors: Tonum Kanlayanee , Srimai Nipitpon , Chabang Napason , Fongsupa Somsak , Tuchinda Patoomratana , Torres Jacob A. , Weimbs Thomas , Soodvilai Sunhapas
Published: April 2022

Renal cyst expansion in polycystic kidney disease (PKD) involves abnormalities in both cyst-lining-cell proliferation and fluid accumulation. Suppression of these processes may retard the progression of PKD. Evidence suggests that the activation of 5′ AMP-activated protein kinase (AMPK) inhibits cystic fibrosis transmembrane conductance regulator (CFTR)–mediated chloride secretion, leading to reduced progression of PKD. Here we investigated the pharmacological effects of panduratin A, a bioactive compound known as an AMPK activator, on CFTR-mediated chloride secretion and renal cyst development using in vitro and animal models of PKD. We demonstrated that AMPK was activated in immortalized normal renal cells and autosomal dominant polycystic kidney disease (ADPKD) cells following treatment with panduratin A. Treatment with panduratin A reduced the number of renal cyst colonies corresponding with a decrease in cell proliferation and phosphorylated p70/S6K, a downstream target of mTOR signaling. Additionally, panduratin A slowed cyst expansion via inhibition of the protein expression and transport function of CFTR. In heterozygous Han:Sprague–Dawley (Cy/+) rats, an animal model of PKD, intraperitoneal administration of panduratin A (25 mg/kg BW) for 5 weeks significantly decreased the kidney weight per body weight ratios and the cystic index. Panduratin A also reduced collagen deposition in renal tissue. Intraperitoneal administration of panduratin A caused abdominal bleeding and reduced body weight. However, 25 mg/kg BW of panduratin A via oral administration in the PCK rats, another non-orthologous PKD model, showed a significant decrease in the cystic index without severe adverse effects, indicating that the route of administration is critical in preventing adverse effects while still slowing disease progression. These findings reveal that panduratin A might hold therapeutic properties for the treatment of PKD.
Proteomic Analysis of Microorganisms
Authors: Kim Seung Il
Published: April 2022

At the early stage of the development of proteomic technologies, Escherichia coli or Saccharomyces cerevisiae were used as model microorganisms for high-throughput identification technologies, such as shotgun proteomics or 2D gel electrophoresis-based LC-MS/MS analysis [...]
Conductive Supramolecular Polymer Nanocomposites with Tunable Properties to Manipulate Cell Growth and Functions
Authors: Wu Cheng-You , Melaku Ashenafi Zeleke , Ilhami Fasih Bintang , Chiu Chih-Wei , Cheng Chih-Chia
Published: April 2022

Synthetic bioactive nanocomposites show great promise in biomedicine for use in tissue growth, wound healing and the potential for bioengineered skin substitutes. Hydrogen-bonded supramolecular polymers (3A-PCL) can be combined with graphite crystals to form graphite/3A-PCL composites with tunable physical properties. When used as a bioactive substrate for cell culture, graphite/3A-PCL composites have an extremely low cytotoxic activity on normal cells and a high structural stability in a medium with red blood cells. A series of in vitro studies demonstrated that the resulting composite substrates can efficiently interact with cell surfaces to promote the adhesion, migration, and proliferation of adherent cells, as well as rapid wound healing ability at the damaged cellular surface. Importantly, placing these substrates under an indirect current electric field at only 0.1 V leads to a marked acceleration in cell growth, a significant increase in total cell numbers, and a remarkable alteration in cell morphology. These results reveal a newly created system with great potential to provide an efficient route for the development of multifunctional bioactive substrates with unique electro-responsiveness to manipulate cell growth and functions.
Analysis of microRNAs in Small Urinary Extracellular Vesicles and Their Potential Roles in Pathogenesis of Renal ANCA-Associated Vasculitis
Authors: Frydlova Jana , Zednikova Iveta , Satrapova Veronika , Pazourkova Eva , Santorova Sarka , Hruskova Zdenka , Tesar Vladimir , Vokurka Martin , Prikryl Petr , Korabecna Marie
Published: April 2022

Antineutrophil cytoplasmic antibodies (ANCA)-associated vasculitis (AAV) represents an autoimmunity disease characterized by high mortality. For successful treatment, the detailed knowledge of its complex pathogenesis and the set of biomarkers for differential diagnostics are desired. Analysis of molecular content of small urinary extracellular vesicles (uEV) offers the possibility to find markers in the form of microRNAs (miRNAs) and study the pathways involved in pathogenesis. We used next-generation sequencing in the first preliminary study to detect the miRNAs with altered expression in uEVs of patients with AAV in comparison with age-matched controls. We confirmed the results using single-target quantitative polymerase chain reaction tests on different sets of samples and found five miRNAs (miR-30a-5p, miR-31-3p, miR-99a-5p, miR-106b-5p, miR-182-5p) with highly elevated levels in uEVs of patients. We performed the comparison of their targets with the differentially expressed proteins in uEVs of patients included in the first phase. We realized that upregulated miRNAs and proteins in uEVs in AAV patients target different biological pathways. The only overlap was detected in pathways regulating the actin cytoskeleton assembly and thus potentially affecting the glomerular functions. The associations of upregulated miRNAs with pathways that were neglected as components of complex AAV pathogenesis, e.g., the epidermal growth factor receptor signaling pathway, were found.
Quality Control of Bacterial Extracellular Vesicles with Total Protein Content Assay, Nanoparticles Tracking Analysis, and Capillary Electrophoresis
Authors: Steć Aleksandra , Jońca Joanna , Waleron Krzysztof , Waleron Małgorzata , Płoska Agata , Kalinowski Leszek , Wielgomas Bartosz , Dziomba Szymon
Published: April 2022

Extracellular vesicles (EVs) were isolated from Pectobacterium zantedeschiae culturing media using direct ultracentrifugation (UC), iodixanol cushion ultracentrifugation (ICUC), and iodixanol density gradient ultracentrifugation (IDGUC) techniques. The isolates were characterized with total protein content assay (bicinchoninic acid assay, BCA), nanoparticles tracking analysis (NTA), and capillary electrophoresis (CE). A satisfactory correlation (R2 > 0.94) between quantitative results obtained with BCA, NTA and CE was achieved only for isolates obtained with the IDGUC. The correlation between protein content and CE was proved to be related to the isolates’ purity. The NTA was found unable to provide reliable information on EVs quantity in samples isolated with UC and ICUC, due to the co-isolated particulate impurities. Moreover, the work reports polysaccharides, used as culturing media components, as a potential source of bias of quantitation with total protein content assay and NTA. The study demonstrates the advantageous selectivity of CE in quality control of EVs and its ability to differentiate subpopulations of EVs of Pectobacterium.
Transcriptome Analyses of Adipose Tissue Samples Identify EGFL6 as a Candidate Gene Involved in Obesity-Related Adipose Tissue Dysfunction in Children
Authors: Landgraf Kathrin , Kühnapfel Andreas , Schlanstein Maria , Biemann Ronald , Isermann Berend , Kempf Elena , Kirsten Holger , Scholz Markus , Körner Antje
Published: April 2022

Obesity develops early in childhood and is accompanied by early signs of adipose tissue (AT) dysfunction and metabolic disease in children. In order to analyse the molecular processes during obesity-related AT accumulation in children, we investigated genome-wide expression profiles in AT samples, isolated adipocytes, and stromal vascular fraction (SVF) cells and assessed their relation to obesity as well as biological and functional AT parameters. We detected alterations in gene expression associated with obesity and related parameters, i.e., BMI SDS, adipocyte size, macrophage infiltration, adiponectin, and/or leptin. While differential gene expression in AT and adipocytes shared an enrichment in metabolic pathways and pathways related to extracellular structural organisation, SVF cells showed an overrepresentation in inflammatory pathways. In adipocytes, we found the strongest positive association for epidermal growth factor-like protein 6 (EGFL6) with adipocyte hypertrophy. EGFL6 was also upregulated during in vitro adipocyte differentiation. In children, EGFL6 expression was positively correlated to parameters of AT dysfunction and metabolic disease such as macrophage infiltration into AT, hs-CRP, leptin levels, and HOMA-IR. In conclusion, we provide evidence for early alterations in AT gene expression related to AT dysfunction in children and identified EGFL6 as potentially being involved in processes underlying the pathogenesis of metabolic disease.
Neuroprotective Potential of Dendritic Cells and Sirtuins in Multiple Sclerosis
Authors: Piacente Francesco , Bottero Marta , Benzi Andrea , Vigo Tiziana , Uccelli Antonio , Bruzzone Santina , Ferrara Giovanni
Published: April 2022

Myeloid cells, including parenchymal microglia, perivascular and meningeal macrophages, and dendritic cells (DCs), are present in the central nervous system (CNS) and establish an intricate relationship with other cells, playing a crucial role both in health and in neurological diseases. In this context, DCs are critical to orchestrating the immune response linking the innate and adaptive immune systems. Under steady-state conditions, DCs patrol the CNS, sampling their local environment and acting as sentinels. During neuroinflammation, the resulting activation of DCs is a critical step that drives the inflammatory response or the resolution of inflammation with the participation of different cell types of the immune system (macrophages, mast cells, T and B lymphocytes), resident cells of the CNS and soluble factors. Although the importance of DCs is clearly recognized, their exact function in CNS disease is still debated. In this review, we will discuss modern concepts of DC biology in steady-state and during autoimmune neuroinflammation. Here, we will also address some key aspects involving DCs in CNS patrolling, highlighting the neuroprotective nature of DCs and emphasizing their therapeutic potential for the treatment of neurological conditions. Recently, inhibition of the NAD+-dependent deac(et)ylase sirtuin 6 was demonstrated to delay the onset of experimental autoimmune encephalomyelitis, by dampening DC trafficking towards inflamed LNs. Thus, a special focus will be dedicated to sirtuins’ role in DCs functions.
Nucleic Acids as Biotools at the Interface between Chemistry and Nanomedicine in the COVID-19 Era
Authors: Borbone Nicola , Piccialli Ilaria , Falanga Andrea Patrizia , Piccialli Vincenzo , Roviello Giovanni N. , Oliviero Giorgia
Published: April 2022

The recent development of mRNA vaccines against the SARS-CoV-2 infection has turned the spotlight on the potential of nucleic acids as innovative prophylactic agents and as diagnostic and therapeutic tools. Until now, their use has been severely limited by their reduced half-life in the biological environment and the difficulties related to their transport to target cells. These limiting aspects can now be overcome by resorting to chemical modifications in the drug and using appropriate nanocarriers, respectively. Oligonucleotides can interact with complementary sequences of nucleic acid targets, forming stable complexes and determining their loss of function. An alternative strategy uses nucleic acid aptamers that, like the antibodies, bind to specific proteins to modulate their activity. In this review, the authors will examine the recent literature on nucleic acids-based strategies in the COVID-19 era, focusing the attention on their applications for the prophylaxis of COVID-19, but also on antisense- and aptamer-based strategies directed to the diagnosis and therapy of the coronavirus pandemic.
Madecassic Acid—A New Scaffold for Highly Cytotoxic Agents
Authors: Kraft Oliver , Hartmann Ann-Kathrin , Hoenke Sophie , Serbian Immo , Csuk René
Published: April 2022

Due to their manifold biological activities, natural products such as triterpenoids have advanced to represent excellent leading structures for the development of new drugs. For this reason, we focused on the syntheses and cytotoxic evaluation of derivatives obtained from gypsogenin, hederagenin, and madecassic acid, cytotoxicity increased—by and large—from the parent compounds to their acetates. Another increase in cytotoxicity was observed for the acetylated amides (phenyl, benzyl, piperazinyl, and homopiperazinyl), but a superior cytotoxicity was observed for the corresponding rhodamine B conjugates derived from the (homo)-piperazinyl amides. In particular, a madecassic acid homopiperazinyl rhodamine B conjugate 24 held excellent cytotoxicity and selectivity for several human tumor cell lines. Thus, this compound was more than 10,000 times more cytotoxic than parent madecassic acid for A2780 ovarian cancer cells. We assume that the presence of an additional hydroxyl group at position C–6 in derivatives of madecassic, as well as the (2α, 3β) configuration of the acetates in ring A, had a beneficial effect onto the cytotoxicity of the conjugates, as well as onto tumor/non-tumor cell selectivity.
Oxygen and Drug-Carrying Periodic Mesoporous Organosilicas for Enhanced Cell Viability under Normoxic and Hypoxic Conditions
Authors: Kumar Ravi , Kehr Nermin Seda
Published: April 2022

Over the last decade, inorganic/organic hybrids have been exploited for oxygen-carrying materials and drug delivery. Its low-cost synthesis, controlled shape and size, and stability have made it a viable delivery strategy for therapeutic agents. Rutin (quercetin-3-O-rutinoside) is a bioflavonoid found in fruits and vegetables. Rutin has a variety of pharmaceutical applications, but its low water solubility reduces its stability and bioavailability. As a result, we introduce a new and stable nanosystem for loading a low-soluble drug (rutin) into oxygen-carrying periodic mesoporous organosilicas (PMO-PFCs). Over the course of 14 days, this nanosystem provided a sustained oxygen level to the cells in both normoxic and hypoxic conditions. At different pH values, the drug release (rutin) profile is also observed. Furthermore, the rutin-coated PMO-PFCs interacted with both healthy and malignant cells. The healthy cells have better cell viability on the rutin-coated oxygen-carrying PMO-PFCs, while the malignant cells have a lower cell viability.
Starch Degradation and Sucrose Accumulation of Lily Bulbs after Cold Storage
Authors: Yu Junpeng , Xu Sujuan , Liu Xinyue , Li Ting , Zhang Dehua , Teng Nianjun , Wu Ze
Published: April 2022

Functional lilies are a group of edible lily cultivars with great potential for landscape application. Low-temperature storage can significantly improve their taste, but the knowledge of this process is largely unknown. In this study, we used the functional lilies ‘Fly Shaohua’ and ‘Fly Tiancheng’ as materials. Through physiological observation and transcriptome analysis during the bulbs’ cold storage, it was found that the starch degradation and sucrose accumulation in bulbs contributed to taste improvement. After 60 d of cold storage, the sucrose accumulation was highest and the starch content was lower in the bulbs, suggesting this time-point was optimal for consumption. Accompanying the fluctuation of sucrose content during cold storage, the enzyme activities of sucrose phosphate synthase and sucrose synthase for sucrose synthesis were increased. Transcriptome analysis showed that many differentially expressed genes (DEGs) were involved in the starch and sucrose metabolism pathway, which might promote the conversion of starch to sucrose in bulbs. In addition, the DEGs involved in dormancy and stress response were also determined during cold storage, which might explain the decreased sucrose accumulation with extended storage time over 60 d due to the energy consumption for dormancy release. Taken together, our results indicated sucrose accumulation was a main factor in the taste improvement of lily bulbs after cold storage, which is attributable to the different gene expression of starch and sucrose metabolism pathways in this process.
Concurrent Chemoradiotherapy-Driven Cell Plasticity by miR-200 Family Implicates the Therapeutic Response of Esophageal Squamous Cell Carcinoma
Authors: Lee Yu-Cheng , Lin Cheng-Han , Chang Wei-Lun , Lin Wen-Der , Pan Jhih-Kai , Wang Wei-Jan , Su Bor-Chyuan , Chung Hsien-Hui , Tsai Chen-Hsun , Lin Forn-Chia , Wang Wen-Ching , Lu Pei-Jung
Published: April 2022

Esophageal squamous cell carcinoma (ESCC) is a common and fatal malignancy with an increasing incidence worldwide. Over the past decade, concurrent chemoradiotherapy (CCRT) with or without surgery is an emerging therapeutic approach for locally advanced ESCC. Unfortunately, many patients exhibit poor response or develop acquired resistance to CCRT. Once resistance occurs, the overall survival rate drops down rapidly and without proper further treatment options, poses a critical clinical challenge for ESCC therapy. Here, we utilized lab-created CCRT-resistant cells as a preclinical study model to investigate the association of chemoradioresistantresistance with miRNA-mediated cell plasticity alteration, and to determine whether reversing EMT status can re-sensitize refractory cancer cells to CCRT response. During the CCRT treatment course, refractory cancer cells adopted the conversion of epithelial to mesenchymal phenotype; additionally, miR-200 family members were found significantly down-regulated in CCRT resistance cells by miRNA microarray screening. Down-regulated miR-200 family in CCRT resistance cells suppressed E-cadherin expression through snail and slug, and accompany with an increase in N-cadherin. Rescuing expressions of miR-200 family members in CCRT resistance cells, particularly in miR-200b and miR-200c, could convert cells to epithelial phenotype by increasing E-cadherin expression and sensitize cells to CCRT treatment. Conversely, the suppression of miR-200b and miR-200c in ESCC cells attenuated E-cadherin, and that converted cells to mesenchymal type by elevating N-cadherin expression, and impaired cell sensitivity to CCRT treatment. Moreover, the results of ESCC specimens staining established the clinical relevance that higher N-cadherin expression levels associate with the poor CCRT response outcome in ESCC patients. Conclusively, miR-200b and miR-200c can modulate the conversion of epithelial–mesenchymal phenotype in ESCC, and thereby altering the response of cells to CCRT treatment. Targeting epithelial–mesenchymal conversion in acquired CCRT resistance may be a potential therapeutic option for ESCC patients.
Digital Transformation Strategies Enabled by Internet of Things and Big Data Analytics: The Use-Case of Telecommunication Companies in Greece
Authors: Moumtzidis Ilias , Kamariotou Maria , Kitsios Fotis
Published: April 2022

Both Internet of Things (IoT) and Big Data Analytics (BDA) are innovations that already caused a significant disruption having a major impact on organizations. To reduce the attrition of new technology implementation, it is critical to examine the advantages of BDA and the determinants that have a detrimental or positive impact on users’ attitudes toward information systems. This article aims to evaluate the intention to use and the perceived benefits of BDA systems and IoT in the telecommunication industry. The research is based on the Technology Acceptance Model (TAM). Data were collected by 172 users and analyzed using Multivariate Regression Analysis. From our findings, we may draw some important lessons about how to increase the adoption of new technology and conventional practices while also considering a variety of diverse aspects. Users will probably use both systems if they think they will be valuable and easy to use. Regarding BDA, the good quality of data helps users see the system’s benefits, while regarding IoT, the high quality of the services is the most important thing.
An Effective Student Grouping and Course Recommendation Strategy Based on Big Data in Education
Authors: Guo Yu , Chen Yue , Xie Yuanyan , Ban Xiaojuan
Published: April 2022

Personalized education aims to provide cooperative and exploratory courses for students by using computer and network technology to construct a more effective cooperative learning mode, thus improving students’ cooperation ability and lifelong learning ability. Based on students’ interests, this paper proposes an effective student grouping strategy and group-oriented course recommendation method, comprehensively considering characteristics of students and courses both from a statistical dimension and a semantic dimension. First, this paper combines term frequency–inverse document frequency and Word2Vec to preferably extract student characteristics. Then, an improved K-means algorithm is used to divide students into different interest-based study groups. Finally, the group-oriented course recommendation method recommends appropriate and quality courses according to the similarity and expert score. Based on real data provided by junior high school students, a series of experiments are conducted to recommend proper social practical courses, which verified the feasibility and effectiveness of the proposed strategy.
Local Transformer Network on 3D Point Cloud Semantic Segmentation
Authors: Wang Zijun , Wang Yun , An Lifeng , Liu Jian , Liu Haiyang
Published: April 2022

Semantic segmentation is an important component in understanding the 3D point cloud scene. Whether we can effectively obtain local and global contextual information from points is of great significance in improving the performance of 3D point cloud semantic segmentation. In this paper, we propose a self-attention feature extraction module: the local transformer structure. By stacking the encoder layer composed of this structure, we can extract local features while preserving global connectivity. The structure can automatically learn each point feature from its neighborhoods and is invariant to different point orders. We designed two unique key matrices, each of which focuses on the feature similarities and geometric structure relationships between the points to generate attention weight matrices. Additionally, the cross-skip selection of neighbors is used to obtain larger receptive fields for each point without increasing the number of calculations required, and can therefore better deal with the junction between multiple objects. When the new network was verified on the S3DIS, the mean intersection over union was 69.1%, and the segmentation accuracies on the complex outdoor scene datasets Semantic3D and SemanticKITTI were 94.3% and 87.8%, respectively, which demonstrate the effectiveness of the proposed methods.
Experimental and Numerical Study on Shear Behavior of Stiffened Thin Steel Plate Shear Walls by New Welding Process
Authors: Xu Han , Duan Kunpeng , Li Chaoqun , Qiang Xuhong , Liu Yutao
Published: April 2022

Steel plate shear walls (SPSW) structures have been widely employed in multistory residential buildings. The traditional welding process may lead to serious welding deformation due to the thinness of the plate. In this study, a new welding process is proposed to ensure that the stiffeners and SPSWs bend as a whole, and the number of welds is reduced from 3 to 2. This process has better integrity than the traditional process owing to less welding residual stress and deformation. On the basis of low-cycle reciprocating load tests on four full-scale specimens, the shear failure pattern, hysteresis characteristics, and load-carrying capacity of SPSWs affected by the new process are studied, and the new welding process used in the vertical stiffener can meet the requirements of shear capacity. The influences of various parameters on the shear resistance of the SPSWs made by the new welding process are compared and analyzed. The results indicate that the lateral stiffness of the frame and the width–height ratios of the wall significantly influence the load-carrying capacity of the SPSWs. The SPSWs adopting the new manufacturing process are numerically simulated using ANSYS software. The same conclusions can be obtained by comparing the numerical results with the experimental results.
Seed Dispersal by Ants in Three Early-Flowering Plants
Authors: Prokop Pavol , Fančovičová Jana , Hlúšková Zuzana
Published: April 2022

Interactions between ants and plants vary from being occasionally beneficial to neutral and negative. Ant-mediated dispersal of obligatory myrmecochorous plants is considered mutualistic interaction, providing benefits to plants in terms of seed dispersal. Ants are rewarded by providing elaiosome, sugar, lipid and protein-rich appendages attached to seeds (diaspores). We experimentally examine rates of diaspore removal rates among three species of plants (snowdrop Galanthus nivalis, hollow root Corydalis cava and European wild ginger Asarum europaeum) under field conditions in two study sites in Central Europe. Diaspore morphology is altered by manipulating both elaiosome and seed size. The small-sized acorn ant Temnothorax crassispinus interacts with the snowdrop and hollow root and the moderately-sized red ant Myrmica ruginodis interacts with European wild ginger. Experimental manipulation with elaiosomes yields largely non-significant results. Diaspore removal rates are generally low (snowdrop 10%, hollow root 26%, European wild ginger 34%) probably due to the small size of ants relative to heavy diaspores. Many ants are observed to consume elaiosomes in situ (cheating). We conclude that ant–plant relationships in this case are not mutualistic but rather neutral/slightly negative, because the plants do not obtain any apparent benefits from their interactions with ants.
The Influence of Planting Periods on Herbivore and Natural Enemy Abundance on Yellow Sticky Traps in Bt Maize Fields
Authors: Clemente Orta Gemma , Álvarez Hugo Alejandro , Madeira Filipe , Albajes Ramon
Published: April 2022

Knowledge of the insect densities during crop development is necessary for adopting appropriate measures for the control of insect pests and minimizing yield losses. Within integrated pest management programs, crop rotation has been carried out in recent years, but this current trend delays the planting period for Bt maize. The small amount of available information regarding the influence of sowing Bt maize early or late on the seasonal abundance of herbivores prompted us to study these aspects in two current common planting periods in northeastern Spain in 52 maize fields over three consecutive years. We sampled the fields planted on different dates with sticky yellow traps. Our results show that only the abundances of herbivore thrips, other than Frankliniella occidentalis, and Syrphidae were significantly different between the two planting periods. Moreover, when we performed yearly analyses, we found significant effects of the planting period on Coccinellidae and Chrysopidae in 2015 and on Aeolothrips sp. in 2016 and 2017. In most of the taxa, the abundance peaks in earlier growth stages, which is related to pollination (before or during). Only the abundances of Stethorus punctillum and Syrphidae peak later in the season. In addition, F. occidentalis, aphids, Syrphidae, and Coccinellidae registered higher abundance in fields sown in the late planting period. These results highlight the effects of sowing in different planting periods on insect dynamics in Bt maize and can be used to identify the abundance of certain pests and natural enemies in specific phenological stages of maize, which may allow producers to adopt better-integrated management and thus avoid reaching the level of economic damage.
Feasibility and Comparison of Resting Full-Cycle Ratio and Computed Tomography Fractional Flow Reserve in Patients with Severe Aortic Valve Stenosis
Authors: Wienemann Hendrik , Langenbach Marcel C. , Mauri Victor , Banazadeh Maryam , Klein Konstantin , Hohmann Christopher , Lee Samuel , Breidert Isabel , Hof Alexander , Eghbalzadeh Kaveh , Kuhn Elmar , Halbach Marcel , Maintz David , Baldus Stephan , Bunck Alexander , Adam Matti
Published: April 2022

Background: Computed tomography derived Fractional Flow Reserve (CT-FFR) has been shown to decrease the referral rate for invasive coronary angiography (ICA). The purpose of the study was to evaluate the diagnostic performance of CT-FFR compared to hyperemia-free index Resting Full-cycle Ratio (RFR) in patients with relevant aortic stenosis (AS) and intermediate coronary stenosis. Methods: 41 patients with 46 coronary lesions underwent ICA with quantitative coronary angiography (QCA), pressure wire assessment and routine pre-transcatheter aortic valve replacement (TAVR) computed tomography (CT). CT-FFR analysis was performed using prototype on-site software. Results: RFR showed a significant correlation with CT-FFR (Pearson’s correlation, r = 0.632, p < 0.001). On a per-lesion basis, diagnostic accuracy, sensitivity, specificity, positive predictive value, and negative predictive value of CT-FFR were 82.6% (95% CI 68.6–92.2), 69.6% (95% CI 47.1–86.8), 95.7% (95% CI  78.1–99.9), 94.1% (95% CI 69.8–99.1), and 75.9% (95% CI 62.7–85.4), respectively. The optimal cutoff value of the CT-FFR for RFR ≤ 0.89 prediction was 0.815. The area under the receiver curve showed a larger area under the curve for CT-FFR (0.87; 95% CI 0.75–0.98) compared with CTA stenosis of ≥50% (0.54, 95% CI 0.38–0.71), CTA ≥ 70% (0.72, 95% CI 0.57–0.87) and QCA ≥ 50% (0.67, 95% CI 0.52–0.83). Conclusions: CT-FFR assessed by routine pre-TAVR CT is safe and feasible and shows a significant correlation with RFR in patients with AS. CT-FFR is superior to QCA ≥ 50%, CT ≥ 50% and CT ≥ 70% in assessing the hemodynamic relevance of intermediate coronary lesions. Thus, CT-FFR has the potential to guide revascularization in patients with AS.
Phase I Targeted Combination Trial of Sorafenib and GW5074 in Patients with Advanced Refractory Solid Tumors
Authors: Kao Chien-Chang , Ho Ching-Liang , Yang Ming-Hsin , Tsai Yi-Ta , Liu Shu-Yu , Chang Ping-Ying , Wu Yi-Ying , Chen Jia-Hong , Huang Tzu-Chuan , Yehn Ren-Hua , Dai Ming-Shen , Chen Yeu-Chin , Sun Guang-Huan , Cha Tai-Lung
Published: April 2022

Background: Combination therapy with the administration of GW5074 and sorafenib significantly induced necrotic death in various cancer cells in vivo, as well as prolonging the survival of an animal disease model due to significant suppression of the primary and metastatic lesions. We sought to determine the safety, tolerability, pharmacokinetics, and anti-tumor activity of this co-administration therapy in patients with refractory advanced solid cancers. Methods: Twelve patients were enrolled. Eligible subjects received different dosages of GW5074 in one of the three dose cohorts (Cohort 1: 750 mg daily, Cohort 2: 1500 mg daily, Cohort 3: 750 mg twice daily) plus 200 mg of sorafenib daily to determine the maximum tolerated dose (MTD) and dose limiting toxicities (DLT) at phase 1. Furthermore, the expression level of phosphorylated DAPKS308 in primary tumor, metastatic tumor, and circulating tumor cells (CTC) were evaluated to investigate the relationship between biomarker and the efficacy profile. Results: Among the 12 enrolled patients in this phase 1 trial, most adverse effects (AE) were grade 1, with two being grade 3. The most frequent AE of all grades were weight loss and hypertension, occurring in 16.7% of participants. Eight patients (66.7%) had the disease controlled by receiving co-administration therapy of GW5074 and sorafenib. GW5074 was found to have poor absorption, as increasing the dosage did not result in a significant increase in the bioavailability of GW5074 in subjects. Furthermore, the expression level of phosphorylated DAPKS308 in tumor and CTCs were correlated with the disease control rate (DCR) and duration of response (DOR). Conclusions: Co-administration therapy of GW5074 and sorafenib demonstrated a favorable safety profile and showed anti-tumor activity in a variety of tumor types. However, the solubility of GW5074 is not satisfactory. A future phase 2a trial will be carried out using the new salted form that has been proven to be more effective.