bluuebunny/crossref_metadata_2025 huggingface dataset:
HIGHLY REPRODUCIBLE SINGLE POLYANILINE NANOWIRE USING ELECTROPHORESIS METHOD
Authors: LEE INNAM , PARK HO IL , PARK SEONGYONG , KIM MOON J. , YUN MINHEE
Published: May 2008

Site-specific single polyaniline nanowires were fabricated through electrophoresis growth with acetone wetting. After growing the nanowires, the post-process of acetone wetting of the nanowires improved morphology, topology, and electrical conductivity with coagulation and substitution in polyaniline. They showed resistance changes of 39.57 ± 11.57% and presented 2.38 × 10-4 ± 3 × 10-5 Ω · cm, 133.77 ± 13.82 nm thickness, and 133.17 ± 13.01 nm width in 1 μm to 5.5 μm length. The new combined growth process of electrophoresis and acetone wetting significantly improved reproducibility, reliability, and controllability in the fabrication of single polymer nanowires.
PURITY MEASUREMENT OF SINGLE-WALLED CARBON NANOTUBES BY UV-VIS-NIR ABSORPTION SPECTROSCOPY AND THERMOGRAVIMETRIC ANALYSIS
Authors: JEONG MUN SEOK , BYEON CLARE C. , CHA OK HWAN , JEONG HYUN , HAN JONG HUN , CHOI YOUNG CHUL , AN KAY HYEOK , OH KYUNG HUI , KIM KI KANG , LEE YOUNG HEE
Published: May 2008

We measured the content of single-walled carbon nanotubes (SWCNTs) in SWCNT soot within 3.7% error using UV-VIS-NIR absorption spectroscopy. We also propose a better overall evaluation method by combining thermogravimetic analysis with UV-VIS-NIR absorption spectroscopy to analyze the purity of SWCNT providing the accurate assessment of the amounts of noncarbonaceous impurity, carbonaceous impurity, and SWCNT each with respect to a highly purified reference.
The boundaries of the West African craton, with special reference to the basement of the Moroccan metacratonic Anti-Atlas belt
Authors: Ennih Nasser , Liégeois Jean-Paul
Published: May 2008

The West African craton (WAC) was constructed during the Archaean and the
c.
2 Ga Palaeoproterozoic Eburnian orogeny. Mesoproterozoic quiescence at
c.
1.7–1.0 Ga allowed cratonization. In the absence of Mesoproterozoic activity, there are no known WAC palaeogeographical positions for that time. At the beginning of the Neoproterozoic, the WAC was affected by several extensional events suggesting that it was subjected to continental breakup. The most important event is the formation of the Gourma aulacogen in Mali, and the Taoudeni cratonic subcircular basin and deposition of platform sediments in the Anti-Atlas. At the end of the Neoproterozoic, the WAC was subjected to convergence on all its boundaries, from the north in the Anti-Atlas, to the east along the Trans-Saharan belt, to the south along the Rockelides and the Bassarides and to the east along the Mauritanides. This led to a partial remobilization of its cratonic boundaries giving rise to a metacratonic evolution. The WAC boundaries experienced Pan-African Neoproterozoic to Early Cambrian transpression and transtension, intrusion of granitoids and extrusion of huge volcanic sequences in such as in the Anti-Atlas (Ouarzazate Supergroup). Pan-African tectonism generated large sediment influxes around the WAC within the Peri-Gondwanan terranes whose sedimentary sequences are marked by distinctive zircon ages of 1.8–2.2 Ga and 0.55–0.75 Ga.

WAC rocks experienced Pan-African low grade metamorphism and large movements of mineralizing fluids. In the Anti-Atlas, this Pan-African metacratonic evolution led to remobilization of REE in the Eburnian granitoids due to the activity of F-rich fluids linked to extrusion of the Ouarzazate Supergroup. During the Phanerozoic, the western WAC boundary was subjected to the Variscan orogeny, for which it constituted the foreland and was, therefore moderately affected, showing typical thick-skin tectonics in the basement and thin-skin tectonics in the cover. During the Mesozoic, the eastern and southern boundaries of the WAC were subjected to the Atlantic opening including Jurassic dolerite intrusion and capture of its extreme southern tip by South America. The Jurassic is also marked by the development of rifts on its eastern and northern sides (future Atlas belt). Finally, the Cenozoic period was marked by the convergence of the African and European continents, generating the High Atlas range and Cenozoic volcanism encircling the northern part of the WAC. The northern metacratonic boundary of the WAC is currently uplifted, forming the Anti-Atlas Mountains.

The boundaries of the WAC, metacratonized during the Pan-African orogeny have been periodically rejuvenated. This is a defining characteristic of the metacratonic areas: rigid, stable cratonic regions that can be periodically cut by faults and affected by magmatism and hydrothermal alteration – making these areas important for mineralization.
Gold mineralization in the Proterozoic Bleida ophiolite, Anti-Atlas, Morocco
Authors: Belkabir Abdelhay , Jébrak Michel , Maacha Lhou , Azizi Samir M. Rachid , Madi Atmane
Published: May 2008

The newly discovered (1998) West Bleida gold mineralization (3 tonnes metal Au) lies west of the main Moroccan Bleida copper deposit (1981–1991) in the central Anti-Atlas (southern Morocco). It is hosted by metamorphosed and deformed mafic to intermediate volcanic rocks that are part of the Neoproterozoic tholeiitic volcanosedimentary series forming the stratigraphically upper part of the Bou Azzer ophiolite sequence. Strong sericitization and local silicification are associated with mineralization. These altered rocks represent a proximal hydrothermal alteration halo around the West Bleida ore zones. Normative chlorite characterizes the metamorphic assemblage away from the ore zones. Gold mineralization primarily occurs as deformed gold-bearing quartz veins and disseminations in Cu-rich chert zones (chalcopyrite–malachite), Fe-rich lithofacies and breccia zones. Gold is accompanied by small amounts of copper sulphides (<1% modal chalcopyrite). Scanning electron microscope–energy dispersive spectrometry analyses of gold grains from veins and disseminations reveal the presence of palladium as inclusions of Pd–As–Sb, Pd–Bi–Se and Pd–Te mineral phases. An electron microprobe study confirms the presence of two types of gold. The first is an alloy of Au–Ag–Pd, typically bordered by small grains of Pd and Bi (Te,Sb) phases and associated with a metamorphic assemblage. Isomertieite, Pd
11
(Sb
2
,As
2
), was identified as one of the phases. The second type of gold is electrum (10% Ag, 90% Au), which is always associated with fractures and occurs with hematite and white mica. Based on its form and habits, West Bleida gold reflects two distinct generations of fluid activity. The primary event precipitated Au–Ag–Pd alloys from Au–Pd-bearing hydrothermal fluids and produced auriferous quartz veins and disseminations within mafic rocks of the Bleida ophiolitic accretionary complex. It was structurally and lithologically controlled. This early event is preserved in the deeper (and thus fresher) zones more than 80 m below the surface. Intense tectonic overprinting obscures the genetic relationship between vein and disseminated styles of mineralization, both of which contain Pd-rich gold, but some of the auriferous quartz veins are observed to crosscut disseminated mineralization. Two possible hypotheses are considered: the pre-tectonic root of a volcanogenic massive sulphide system, or a late tectonic orogenic (mesothermal) deposit. The presence of Pd minerals and anomalous cobalt concentrations suggest a source in ultramafic rocks. The second event, characterized by inclusion-free electrum, occurred much later and represents the alteration and weathering of the primary Pd-rich gold assemblage by oxidizing surface fluids. It affected all mineralized units and structures to a depth of 80 m. This post-tectonic surficial alteration also caused leaching of Cu-sulphides, which may explain their low abundances in the upper parts of the ore zones.
Petrology and geochemistry of the Neoproterozoic Siroua granitoids (central Anti-Atlas, Morocco): evolution from subduction-related to within-plate magmatism
Authors: Touil Ahmed , Hafid Ahmid , Moutte Jacques , Boukhari Abdelmajid El
Published: May 2008

The Siroua massif includes many plutons of Neoproterozoic age. The mineralogical and geochemical character of the plutons allows us to describe an evolution of the magmatism, in space and time, from a subduction-related type in the northern part, to a within-plate subalkaline type in the southern part. The first magmatic activity coeval with the closing of the Khzama oceanic basin in the north is little evolved and of oceanic type (dominantly gabbros and basalts). It is followed by a low potassic calc-alkaline magmatism (gabbro–diorites, tonalites and trondhjemites of Nebdas pluton) and by a voluminous highly potassic calcalkaline magmatism (Askaoun and Ifouachguel plutons) that marks the collisional period. The end of crustal uplift and the beginning of the extension is marked in the south by a sub-alkaline magmatism corresponding to the Ida ou Illoun, Imdghar and Affela N'ouassif granites. Magmatic activity, in the Siroua massif, is marked at the end of the Neoproterozoic (PIII) by a continental tholeiite with an alkaline affinity, which occurs as dykes crosscutting the Neoproterozoic granites, and later by dominantly alkaline granites.
The Cambrian volcano-sedimentary formations of the westernmost High Atlas (Morocco): their place in the geodynamic evolution of the West African Palaeo-Gondwana northern margin
Authors: Pouclet A. , Ouazzani H. , Fekkak A.
Published: May 2008

In the westernmost part of the High Atlas, two Palaeozoic formations, rich in mafic volcanic rocks, are distinguished. They belong to different structural blocks created during the Variscan orogeny. New U–Pb dating yields an Early Cambrian age. The basaltic lavas have the composition of continental tholeiites and the magmatic signature of an initial rifting tectonic setting. They are related to the western Moroccan Cambrian rift. Their geodynamical context could be a passive margin initiated from an active rift that aborted in the Middle Cambrian.
The magmatic evolution at the Moroccan outboard of the West African craton between the Late Neoproterozoic and the Early Palaeozoic
Authors: Ezzouhairi H. , Ribeiro M. L. , Ait Ayad N. , Moreira M. E. , Charif A. , Ramos J. M. F. , de Oliveira D. P. S. , Coke C.
Published: May 2008

The Late Neoproterozoic Ouarzazate Group crops out on the north margin of the West African craton (WAC). In this group an important post-collisional magmatism is characterized by a great diversity in plutonic and volcanic rock types of the high-K calc-alkaline series. This series evolved mainly by crystal fractionation and by an important crustal contamination from an anomalous mantle source. The Early Cambrian magmatism began at the same time on both sides of the Anti-Atlas Major Fault, the southwestern side (Kerdous region) and northeastern side (Ouarzazate-Agdz region), interbedded in the Early Cambrian Basal Series and spread later to the Western High Atlas of the Morocco northern WAC outboard areas. This magmatism changes from a continental tholeiitic series (HPT and LPT) at the beginning to an alkaline series at the top (Adoudou and ‘Lie de vin’ formations). Fractional crystallization and pelagic or crustal contamination were the most important processes in the magma differentiation. The geochemical inversion from calc-alkaline to tholeiitic magmatism between the Late Neoproterozoic and the Early Cambrian is documented, as is the major extension of the tholeiitic activity on both sides of the South Atlas Fault. This geochemical variation indicates a transition of the tectonic regime from compressive to extensional. The late local Jbel Boho alkaline magmatism indicates the sink of the source and the mitigation or closure of the extensional cycle at this time.
Zircon U–Pb geochronology of paragneisses and biotite granites from the SW Iberian Massif (Portugal): evidence for a palaeogeographical link between the Ossa–Morena Ediacaran basins and the West African craton
Authors: Pereira M. F. , Chichorro M. , Williams I. S. , Silva J. B.
Published: May 2008

Sensitive high-resolution ion microprobe U–Th–Pb age determinations on detrital and inherited zircon from the Évora Massif (SW Iberian Massif, Portugal) provide direct evidence for the provenance of the Ossa–Morena Ediacaran basins (Série Negra) and a palaeogeographical link with the West African craton. Three samples of the Série Negra paragneisses contain large components of Cryogenian and Ediacaran (
c
. 700–540 Ma) detrital zircon, but have a marked lack of zircon of Mesoproterozoic (
c
. 1.8–0.9 Ga) age. Older inherited zircons are of Palaeoproterozoic (
c
. 2.4–1.8 Ga) and Archaean (
c
. 3.5–2.5 Ga) age. The same age pattern is also found in the Arraiolos biotite granite, which was formed by partial melting of the Série Negra and overlying Cambrian rocks. These results are consistent with substantial denudation of a continental region that supplied sediments to the Ediacaran Ossa–Morena basins during the final stages of the Cadomian–Avalonian orogeny (peri-Gondwanan margin with principal zircon-forming events at
c
. 575 Ma and
c
. 615 Ma). Combined with the detrital zircon ages reported for rocks of the same age from Portugal, Spain, Germany and Algeria, our data suggest that the sediment supply to the Ediacaran–Early Palaeozoic siliciclastic sequences preserved in all these peri-Gondwanan regions was similar. The lack of Grenvillian-aged (
c
. 1.1–0.9 Ga) zircon in the Ossa–Morena and Saxo-Thuringia Ediacaran sediments suggests that the sediment in these peri-Gondwanan basins was derived from the West African craton.
Orogen-parallel tectonic transport: transpression and strain partitioning in the Mauritanides of NE Senegal
Authors: Dabo M. , Gueye M. , Ngom P. M. , Diagne M.
Published: May 2008

New structural, metamorphic, finite-strain analysis and kinematic data from the southern part of the Mauritanides in Senegal (Bakel area) have revealed a history reflecting different tectonic regimes. The structural framework is expressed in the form of shear-zone systems comprising rectilinear NE–SW trending thrusts and NE–SW-trending ductile sinistral strike-slip faults associated with north–south- to NE–SW-trending transpression belts. The analysis of ductile fabrics reveals the presence of lineation trajectories that show a change from a tangential deformation towards the marginal zones in the Northern and Southern Domains of the belt to sinistral oblique transpression in the Central Domain. The deformation within the belt is strongly partitioned into shear zones distributed across the belt defining structural domains with accompanying large-scale folds and thrusts. This exemplifies wrench-style tectonics dominated by crustal-scale strike-slip shear zones in the orogen core and temporal and spatial progression outwards to the marginal zones. Thus, the Variscan orogeny in the southern part of the Mauritanides belt in Senegal developed through sinistrally oblique transpressive shearing along the borders of the orogen core leading to the exhumation of ultramafic rocks between large shear zones.
Deep structure of the southeastern margin of the West African craton from seismic reflection data, offshore Ghana
Authors: Attoh Kodjopa , Brown Larry
Published: May 2008

Seismic reflection data, acquired as part of an oil exploration survey offshore Ghana, have been reprocessed to reveal the deep structure of the Proterozoic continental crust on the continental shelf, off West Africa. The seismic profiles presented are located across the Romanche transform margin, which transects the West African craton (WAC) margin where it is bounded by the Pan-African Dahomeyide orogen. The seismic data reveal highly reflective middle–lower crust of the WAC but a nearly transparent upper crust of
c
. 10 km thickness. Bundles of discontinuous reflections are located at the base of the unreflective upper crust and locally define the trough of a synformal structure interpreted as the keel of a Palaeoproterozoic (Birimian) greenstone belt exposed onshore. The deepest crust imaged is characterized by more continuous, subhorizontal reflections that are very similar to those observed in the lower crust of cratonic areas such as the Superior Province. Near the WAC margin the subhorizontal reflections are truncated by east-dipping reflections in the Pan-African domain, which are correlated with cratonward directed fold-and-thrust structures formed during the
c
. 600 Ma Pan-African orogeny. These seismic observations represent, to date, the first deep seismic reflection images of the West African craton margin and the continental lithosphere in the region.
Geochronology and metamorphic
P
-
T
-
X
evolution of the Eburnean granulite-facies metapelites of Tidjenouine (Central Hoggar, Algeria): witness of the LATEA metacratonic evolution
Authors: Bendaoud Abderrahmane , Ouzegane Khadidja , Godard Gaston , Liégeois Jean-Paul , Kienast Jean-Robert , Bruguier Olivier , Drareni Amar
Published: May 2008

Central Hoggar, within the Tuareg shield to the east of the West African craton, is known for its complexity owing to the interplay of the Eburnean and Pan-African orogenies. The Tidjenouine area in the Laouni terrane belongs to the LATEA metacraton and displays spectacular examples of granulite-facies migmatitic metapelites. Here, we present a detailed petrological study coupled with
in situ
U–Pb zircon dating by laser-ablation inductively coupled plasma mass spectrometry (ICP-MS) that allows us to constrain the relative role of the Eburnean and Pan-African orogenies and hence to constrain how the LATEA Eburnean microcontinent has been partly destabilized during the Pan-African orogeny; that is, its metacratonic evolution. These metapelites have recorded different metamorphic stages. A clockwise P–T evolution is demonstrated on the basis of textural relationships, modelling in KFMASH and FMASH systems and thermobarometry. The prograde evolution implies several melting reactions involving the breakdown of biotite and gedrite. Peak metamorphic
P–T
conditions of 860±50 °C and 7–8 kbar (M
1
) were followed by a decrease of pressure to 4.3±1 kbar and of temperature to around 700 °C, associated with the development of migmatites (M
2
). After cooling, a third thermal phase at
c
. 650 °C and 3–4 kbar (M
3
) occurred. U–Pb zircon laser ablation ICP-MS analysis allows us to date the protolith of the migmatites at 2151±8 Ma, the granulite-facies and migmatitic metamorphisms (M
1
–M
2
) at 2062±39 Ma and the medium-grade metamorphic assemblage (M
3
) at 614±11 Ma. This last event is coeval with the emplacement of large Pan-African granitic batholiths. These data show that the main metamorphic events are Eburnean in age. The Pan-African orogeny, in contrast, is associated mainly with medium-grade metamorphism but also mega-shear zones and granitic batholiths, characterized by a high temperature gradient. This can be considered as typical of a metacratonic evolution.
Review of the orogenic belts on the western side of the West African craton: the Bassarides, Rokelides and Mauritanides
Authors: Villeneuve Michel
Published: May 2008

The West African craton is fringed along its western side by a 3000 km long orogenic belt subdivided into three separate orogens: the Bassaride (Pan-African I orogeny), Rokelide (Pan-African II orogeny) and Mauritanide (Hercynian orogeny) thrust belts. The Bassarides are cut to the north by the Mauritanides and to the south by the Rokelides but parts of this Bassaride belt are incorporated in the other two younger belts. This review presents the main geological, geophysical and geochronological results from the western side of the West African craton, collected over the past 90 years, concentrating on those from the last 15 years. Former geological investigations underlined the thin-skinned structure model within these thrust belts, whereas the geophysical results gave prominence to the major importance of block faulting resulting from the Pan-African I orogeny and its strong influence on the subsequent orogenic belt features. The geochronological data allow us to distinguish major tectonothermal events related to the Pan-African I (660–650 Ma), Pan-African II (550–530 Ma) and Hercynian (330–300 Ma and 280–270 Ma) orogenies. However, they also reveal five other tectonothermal events (at 1200–1000, 750–700, 600–580, 510–480 and 450–380 Ma), which are still very poorly understood. The 1200–1000 Ma tectonothermal event recently revealed in the northern Mauritanides may correspond to a remanent orogenic belt segment that witnessed the Grenvillian orogeny.
Neoproterozoic garnet-glaucophanites and eclogites: new insights for subduction metamorphism of the Gourma fold and thrust belt (eastern Mali)
Authors: Caby R. , Buscail F. , Dembélé D. , Diakité S. , Sacko S. , Bal M.
Published: May 2008

The Neoproterozoic Gourma fold and thrust belt exposed in eastern Mali includes in its inner part high-pressure, low-temperature metasediments and scarce metabasites. This high-pressure metamorphic unit is characterized in the Ansongo region by garnet–glaucophane–paragonite assemblages and eclogites of basaltic derivation, whereas phengite–garnet–rutile mineral assemblages characterize the metapelites. Thermobarometric estimates on the metabasites suggest peak pressure around 13–15 kbar and temperature of 500±50 °C for the Seyna Bela garnet glaucophanite and glaucophane-bearing eclogite, and 16 kbar at 600±50 °C for the Tin Hama phengite eclogite, values indicative of palaeogeothermal gradients of about 10 °C km
−1
typical of subduction settings. The high-pressure unit may represents a giant allochthon emplaced on top of very low-grade metasediments. It represents the southern extension of the ultrahigh-pressure rocks. Garnet pyriclasites from the Amalaoulaou massif, which represents the roots of a
c
. 800–730 Ma Neoproterozoic island arc, underwent a medium-temperature metamorphic overprint characterized by barroisite–paragonite assemblages; that is, of same grade as the decompression that affected the eclogites and garnet-blueschists. The Gourma high-pressure metamorphic belt formed as a consequence of the east-dipping subduction of the Neoproterozoic passive palaeo-margin of the West African craton. The presented
P
–
T
estimates suggest that subduction-related palaeogeothermal gradients during the late Neoproterozoic period along the main Pan-African suture were similar to those reconstructed for Tertiary Alpine-type belts.
Satellite cell proliferation is reduced in muscles of obese Zucker rats but restored with loading
Authors: Peterson Jonathan M. , Bryner Randall W. , Alway Stephen E.
Published: May 2008

The obese Zucker rat (OZR) is a model of metabolic syndrome, which has lower skeletal muscle size than the lean Zucker rat (LZR). Because satellite cells are essential for postnatal muscle growth, this study was designed to determine whether reduced satellite cell proliferation contributes to reduced skeletal mass in OZR vs. LZR. Satellite cell proliferation was determined by a constant-release 5-bromo-2-deoxyuridine (BrdU) pellet that was placed subcutaneously in each animal. Satellite cell proliferation, as determined by BrdU incorporation, was significantly attenuated in control soleus and plantaris muscles of the OZR compared with that shown in the LZR. To determine whether this attenuation of satellite cell activity could be rescued in OZR muscles, soleus and gastrocnemius muscles were denervated, placing a compensatory load on the plantaris muscle. In the LZR and the OZR after 21 days of loading, increases of ∼25% and ∼30%, respectively, were shown in plantaris muscle wet weight compared with that shown in the contralateral control muscle. The number of BrdU-positive nuclei increased similarly in loaded plantaris muscles from LZR and OZR. Myogenin, MyoD, and Akt protein expressions were lower in control muscles of OZR than in those of the LZR, but they were all elevated to similar levels in the loaded plantaris muscles of OZR and LZR. These data indicate that metabolic syndrome may reduce satellite cell proliferation, and this may be a factor that contributes to the reduced mass in control muscles of OZR; however, satellite cell proliferation can be restored with compensatory loading in OZR.
MURC, a muscle-restricted coiled-coil protein, is involved in the regulation of skeletal myogenesis
Authors: Tagawa Masashi , Ueyama Tomomi , Ogata Takehiro , Takehara Naofumi , Nakajima Norio , Isodono Koji , Asada Satoshi , Takahashi Tomosaburo , Matsubara Hiroaki , Oh Hidemasa
Published: May 2008

Skeletal myogenesis is a multistep process by which multinucleated mature muscle fibers are formed from undifferentiated, mononucleated myoblasts. However, the molecular mechanisms of skeletal myogenesis have not been fully elucidated. Here, we identified muscle-restricted coiled-coil (MURC) protein as a positive regulator of myogenesis. In skeletal muscle, MURC was localized to the cytoplasm with accumulation in the Z-disc of the sarcomere. In C2C12 myoblasts, MURC expression occurred coincidentally with myogenin expression and preceded sarcomeric myosin expression during differentiation into myotubes. RNA interference (RNAi)-mediated knockdown of MURC impaired differentiation in C2C12 myoblasts, which was accompanied by impaired myogenin expression and ERK activation. Overexpression of MURC in C2C12 myoblasts resulted in the promotion of differentiation with enhanced myogenin expression and ERK activation during differentiation. During injury-induced muscle regeneration, MURC expression increased, and a higher abundance of MURC was observed in immature myofibers compared with mature myofibers. In addition, ERK was activated in regenerating tissue, and ERK activation was detected in MURC-expressing immature myofibers. These findings suggest that MURC is involved in the skeletal myogenesis that results from modulation of myogenin expression and ERK activation. MURC may play pivotal roles in the molecular mechanisms of skeletal myogenic differentiation.
Abnormal sarcoplasmic reticulum Ca2+-sequestering properties in skeletal muscle in chronic obstructive pulmonary disease
Authors: Green H. J. , Burnett M. , Duhamel T. A. , D'Arsigny C. , O'Donnell D. E. , Webb K. A. , Ouyang J.
Published: May 2008

The objective of this study was to investigate the hypothesis that alterations in sarcoplasmic reticulum (SR) Ca2+-cycling properties would occur in skeletal muscle in patients with moderate to severe chronic obstructive pulmonary disease (COPD). To investigate this hypothesis, tissue samples were obtained from the vastus lateralis of 8 patients with COPD [age 65.6 ± 3.2 yr; forced expiratory volume in 1 s (FEV1)/forced vital capacity (FVC) = 44 ± 2%; mean ± SE] and 10 healthy age-matched controls (CON, age 67.5 ± 2.5 yr; FEV1/FVC = 77 ± 2%), and homogenates were analyzed for a wide range of SR properties. Compared with CON, COPD displayed (in μmol·g protein−1·min−1) a 16% lower maximal Ca2+-ATPase activity [maximal velocity ( Vmax), 158 ± 10 vs. 133 ± 7, P < 0.05] and a 17% lower Ca2+ uptake (4.65 ± 0.039 vs. 3.85 ± 0.26, P < 0.05) that occurred in the absence of differences in Ca2+ release. The lower Vmax in COPD was also accompanied by an 11% lower ( P < 0.05) Ca2+ sensitivity, as measured by the Hill coefficient (defined as the relationship between Ca2+-ATPase activity and free cytosolic Ca2+ concentration for 10–90% Vmax). For the sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA) isoforms, SERCA1a was 16% higher ( P < 0.05) and SERCA2a was 14% lower ( P < 0.05) in COPD. It is concluded that moderate to severe COPD results in abnormalities in SR Ca2+-ATPase properties that cannot be explained by changes in the SERCA isoform phenotypes. The reduced catalytic properties of SERCA in COPD suggest a disturbance in Ca2+ cycling, possibly resulting in impairment in Ca2+-mediated mechanical function and/or second messenger regulated processes.
Small mouse cholangiocytes proliferate in response to H1 histamine receptor stimulation by activation of the IP3/CaMK I/CREB pathway
Authors: Francis Heather , Glaser Shannon , DeMorrow Sharon , Gaudio Eugenio , Ueno Yoshiyuki , Venter Julie , Dostal David , Onori Paolo , Franchitto Antonio , Marzioni Marco , Vaculin Shelley , Vaculin Bradley , Katki Khurshed , Stutes Monique , Savage Jennifer , Alpini Gianfranco
Published: May 2008

Cholangiopathies are characterized by the heterogeneous proliferation of different-sized cholangiocytes. Large cholangiocytes proliferate by a cAMP-dependent mechanism. The function of small cholangiocytes may depend on the activation of inositol trisphosphate (IP3)/Ca2+-dependent signaling pathways; however, data supporting this speculation are lacking. Four histamine receptors exist (HRH1, HRH2, HRH3, and HRH4). In several cells: 1) activation of HRH1 increases intracellular Ca2+ concentration levels; and 2) increased [Ca2+]i levels are coupled with calmodulin-dependent stimulation of calmodulin-dependent protein kinase (CaMK) and activation of cAMP-response element binding protein (CREB). HRH1 agonists modulate small cholangiocyte proliferation by activation of IP3/Ca2+-dependent CaMK/CREB. We evaluated HRH1 expression in cholangiocytes. Small and large cholangiocytes were stimulated with histamine trifluoromethyl toluidide (HTMT dimaleate; HRH1 agonist) for 24–48 h with/without terfenadine, BAPTA/AM, or W7 before measuring proliferation. Expression of CaMK I, II, and IV was evaluated in small and large cholangiocytes. We measured IP3, Ca2+ and cAMP levels, phosphorylation of CaMK I, and activation of CREB (in the absence/presence of W7) in small cholangiocytes treated with HTMT dimaleate. CaMK I knockdown was performed in small cholangiocytes stimulated with HTMT dimaleate before measurement of proliferation and CREB activity. Small and large cholangiocytes express HRH1, CaMK I, and CaMK II. Small (but not large) cholangiocytes proliferate in response to HTMT dimaleate and are blocked by terfenadine (HRH1 antagonist), BAPTA/AM, and W7. In small cholangiocytes, HTMT dimaleate increased IP3/Ca2+ levels, CaMK I phosphorylation, and CREB activity. Gene knockdown of CaMK I ablated the effects of HTMT dimaleate on small cholangiocyte proliferation and CREB activation. The IP3/Ca2+/CaMK I/CREB pathway is important in the regulation of small cholangiocyte function.
Force deficits and breakage rates after single lengthening contractions of single fast fibers from unconditioned and conditioned muscles of young and old rats
Authors: Lynch Gordon S. , Faulkner John A. , Brooks Susan V.
Published: May 2008

The deficit in force generation is a measure of the magnitude of damage to sarcomeres caused by lengthening contractions of either single fibers or whole muscles. In addition, permeabilized single fibers may suffer breakages. Our goal was to understand the interaction between breakages and force deficits in “young” and “old” permeabilized single fibers from control muscles of young and old rats and “conditioned” fibers from muscles that completed a 6-wk program of in vivo lengthening contractions. Following single lengthening contractions of old-control fibers compared with young-control fibers, the twofold greater force deficits at a 10% strain support the concept of an age-related increase in the susceptibility of fibers to mechanical damage. In addition, the much higher breakage rates for old fibers at all strains tested indicate an increase with aging in the number of fibers at risk of being severely injured during any given stretch. Following the 6-wk program of lengthening contractions, young-conditioned fibers and old-conditioned fibers were not different with respect to force deficit or the frequency of breakages. A potential mechanism for the increased resistance to stretch-induced damage of old-conditioned fibers is that, through intracellular damage and subsequent degeneration and regeneration, weaker sarcomeres were replaced by stronger sarcomeres. These data indicate that, despite the association of high fiber breakage rates and large force deficits with aging, the detrimental characteristics of old fibers were improved by a conditioning program that altered both sarcomeric characteristics as well as the overall structural integrity of the fibers.
Sex-specific differences in chromosome-dependent regulation of vascular reactivity in female consomic rat strains from a SS × BN cross
Authors: Kunert Mary Pat , Dwinell Melinda R. , Drenjancevic Peric Ines , Lombard Julian H.
Published: May 2008

High-throughput studies in the Medical College of Wisconsin Program for Genomic Applications (Physgen) were designed to link chromosomes with physiological function in consomic strains derived from a cross between Dahl salt-sensitive SS/JrHsdMcwi (SS) and Brown Norway normotensive BN/NHsdMcwi (BN) rats. The specific goal of the vascular protocol was to characterize the responses of aortic rings from these strains to vasoconstrictor and vasodilator stimuli (phenylephrine, acetylcholine, sodium nitroprusside, and bath hypoxia) to identify chromosomes that either increase or decrease vascular reactivity to these vasoactive stimuli. Because previous studies demonstrated sex-specific quantitative trait loci (QTLs) related to regulation of cardiovascular phenotypes in an F2 cross between the parental strains, males and females of each consomic strain were included in all experiments. As there were significant sex-specific differences in aortic sensitivity to vasoconstrictor and vasodilator stimuli compared with the parental SS strain, we report the results of the females separately from the males. There were also sex-specific differences in aortic ring sensitivity to these vasoactive stimuli in consomic strains that were fed a high-salt diet (4% NaCl) for 3 wk to evaluate salt-induced changes in vascular reactivity. Differences in genetic architecture could contribute to sex-specific differences in the development and expression of cardiovascular diseases via differential regulation and expression of genes. Our findings are the first to link physiological traits with specific chromosomes in female SS rats and support the idea that sex is an important environmental variable that plays a role in the expression and regulation of genes.
Early fetal hypoxia leads to growth restriction and myocardial thinning
Authors: Ream Margie , Ray Alisa M. , Chandra Rashmi , Chikaraishi Dona M.
Published: May 2008

Hypoxia is necessary for fetal development; however, excess hypoxia is detrimental. Hypoxia has been extensively studied in the near-term fetus, but less is known about earlier fetal effects. The purpose of this study was to determine the window of vulnerability to severe hypoxia, what organ system(s) is most sensitive, and why hypoxic fetuses die. We induced hypoxia by reducing maternal-inspired O2from 21% to 8%, which decreased fetal tissue oxygenation assessed by pimonidazole binding. The mouse fetus was most vulnerable in midgestation: 24 h of hypoxia killed 89% of embryonic day 13.5 (E13.5) fetuses, but only 5% of E11.5 and 51% of E17.5 fetuses. Sublethal hypoxia at E12.5 caused growth restriction, reducing fetal weight by 26% and protein by 45%. Hypoxia induced HIF-1 target genes, including vascular endothelial growth factor (Vegf), erythropoietin, glucose transporter-1 and insulin-like growth factor binding protein-1 (Igfbp-1), which has been implicated in human intrauterine growth restriction (IUGR). Hypoxia severely compromised the cardiovascular system. Signs of heart failure, including loss of yolk sac circulation, hemorrhage, and edema, were caused by 18–24 h of hypoxia. Hypoxia induced ventricular dilation and myocardial hypoplasia, decreasing ventricular tissue by 50% and proliferation by 21% in vivo and by 40% in isolated cultured hearts. Epicardial detachment was the first sign of hypoxic damage in the heart, although expression of epicardially derived mitogens, such as FGF2, FGF9, and Wnt9b was not reduced. We propose that hypoxia compromises the fetus through myocardial hypoplasia and reduced heart rate.
Ventilatory responses to acute and chronic hypoxia are altered in female but not male Paskin-deficient mice
Authors: Soliz Jorge , Soulage Christophe , Borter Emanuela , van Patot Martha Tissot , Gassmann Max
Published: May 2008

Proteins harboring a Per-Arnt-Sim (PAS) domain are versatile and allow archaea, bacteria, and plants to sense oxygen partial pressure, as well as light intensity and redox potential. A PAS domain associated with a histidine kinase domain is found in FixL, the oxygen sensor molecule of Rhizobium species. PASKIN is the mammalian homolog of FixL, but its function is far from being understood. Using whole body plethysmography, we evaluated the ventilatory response to acute and chronic hypoxia of homozygous deficient male and female PASKIN mice ( Paskin −/−). Although only slight ventilatory differences were found in males, female Paskin −/− mice increased ventilatory response to acute hypoxia. Unexpectedly, females had an impaired ability to reach ventilatory acclimatization in response to chronic hypoxia. Central control of ventilation occurs in the brain stem respiratory centers and is modulated by catecholamines via tyrosine hydroxylase (TH) activity. We observed that TH activity was altered in male and female Paskin −/− mice. Peripheral chemoreceptor effects on ventilation were evaluated by exposing animals to hyperoxia (Dejours test) and domperidone, a peripheral ventilatory stimulant drug directly affecting the carotid sinus nerve discharge. Male and female Paskin −/− had normal peripheral chemosensory (carotid bodies) responses. In summary, our observations suggest that PASKIN is involved in the central control of hypoxic ventilation, modulating ventilation in a gender-dependent manner.
Acid-sensitive channel inhibition prevents fetal alcohol spectrum disorders cerebellar Purkinje cell loss
Authors: Ramadoss Jayanth , Lunde Emilie R. , Ouyang Nengtai , Chen Wei-Jung A. , Cudd Timothy A.
Published: May 2008

Ethanol is now considered the most common human teratogen. Educational campaigns have not reduced the incidence of ethanol-mediated teratogenesis, leading to a growing interest in the development of therapeutic prevention or mitigation strategies. On the basis of the observation that maternal ethanol consumption reduces maternal and fetal pH, we hypothesized that a pH-sensitive pathway involving the TWIK-related acid-sensitive potassium channels (TASKs) is implicated in ethanol-induced injury to the fetal cerebellum, one of the most sensitive targets of prenatal ethanol exposure. Pregnant ewes were intravenously infused with ethanol (258 ± 10 mg/dl peak blood ethanol concentration) or saline in a “3 days/wk binge” pattern throughout the third trimester. Quantitative stereological analysis demonstrated that ethanol resulted in a 45% reduction in the total number of fetal cerebellar Purkinje cells, the cell type most sensitive to developmental ethanol exposure. Extracellular pH manipulation to create the same degree and pattern of pH fall caused by ethanol (manipulations large enough to inhibit TASK 1 channels), resulted in a 24% decrease in Purkinje cell number. We determined immunohistochemically that TASK 1 channels are expressed in Purkinje cells and that the TASK 3 isoform is expressed in granule cells of the ovine fetal cerebellum. Pharmacological blockade of both TASK 1 and TASK 3 channels simultaneous with ethanol effectively prevented any reduction in fetal cerebellar Purkinje cell number. These results demonstrate for the first time functional significance of fetal cerebellar two-pore domain pH-sensitive channels and establishes them as a potential therapeutic target for prevention of ethanol teratogenesis.
Enhanced bladder capacity and reduced prostaglandin E2-mediated bladder hyperactivity in EP3 receptor knockout mice
Authors: McCafferty Gerald P. , Misajet Blake A. , Laping Nicholas J. , Edwards Richard M. , Thorneloe Kevin S.
Published: May 2008

Nonsteroidal anti-inflammatory cyclooxygenase inhibitors that function to reduce prostaglandin E2 (PGE2) production have been widely reported as effective agents in models of urinary bladder overactivity. We therefore investigated a potential role for the PGE2 receptor, EP3, in urinary bladder function by performing conscious, freely moving cystometry on EP3 receptor knockout (KO) mice. EP3 KO mice demonstrated an enhanced bladder capacity compared with wild-type (WT) mice (∼185% of WT) under control conditions, based on larger voided and infused bladder volumes. Infusion of the EP3 receptor agonist GR63799X into the bladder of WT mice reduced the bladder capacity. This was ineffective in EP3 KO mice that demonstrated a time-dependent increase in bladder capacity with GR63799X, an effect similar to that observed with vehicle in both genotypes. In addition, infusion of PGE2 into WT mice induced bladder overactivity, an effect that was significantly blunted in the EP3 KO mice. The data reported here provide the first evidence supporting a functional role for EP3 receptors in normal urinary bladder function and implicate EP3 as a contributor to bladder overactivity during pathological conditions of enhanced PGE2 production, as reported previously in overactive bladder patients.
V1 Response Timing and Surface Filling-In
Authors: Huang Xin , Paradiso Michael A.
Published: May 2008

There is ample evidence from demonstrations such as color induction and stabilized images that information from surface boundaries plays a special role in determining the perception of surface interiors. Surface interiors appear to “fill-in.” Psychophysical experiments also show that surface perception involves a slow scale-dependent process distinct from mechanisms involved in contour perception. The present experiments aimed to test the hypothesis that surface perception is associated with relatively slow scale-dependent neural filling-in. We found that responses in macaque primary visual cortex (V1) are slower to surface interiors than responses to optimal bar stimuli. Moreover, we found that the response to a surface interior is delayed relative to the response to the surface's border and the extent of the delay is proportional to the distance between a receptive field and the border. These findings are consistent with some forms of neural filling-in and suggest that V1 may provide the neural substrate for perceptual filling-in.
Arousal Facilitates Collision Avoidance Mediated by a Looming Sensitive Visual Neuron in a Flying Locust
Authors: Rind F. Claire , Santer Roger D. , Wright Geraldine A.
Published: May 2008

Locusts have two large collision-detecting neurons, the descending contralateral movement detectors (DCMDs) that signal object approach and trigger evasive glides during flight. We sought to investigate whether vision for action, when the locust is in an aroused state rather than a passive viewer, significantly alters visual processing in this collision-detecting pathway. To do this we used two different approaches to determine how the arousal state of a locust affects the prolonged periods of high-frequency spikes typical of the DCMD response to approaching objects that trigger evasive glides. First, we manipulated arousal state in the locust by applying a brief mechanical stimulation to the hind leg; this type of change of state occurs when gregarious locusts accumulate in high-density swarms. Second, we examined DCMD responses during flight because flight produces a heightened physiological state of arousal in locusts. When arousal was induced by either method we found that the DCMD response recovered from a previously habituated state; that it followed object motion throughout approach; and—most important—that it was significantly more likely to generate the maintained spike frequencies capable of evoking gliding dives even with extremely short intervals (1.8 s) between approaches. Overall, tethered flying locusts responded to 41% of simulated approaching objects (sets of 6 with 1.8 s ISI). When we injected epinastine, the neuronal octopamine receptor antagonist, into the hemolymph responsiveness declined to 12%, suggesting that octopamine plays a significant role in maintaining responsiveness of the DCMD and the locust to visual stimuli during flight.
Asymmetric Recovery in Cerebellar-Deficient Mice Following Unilateral Labyrinthectomy
Authors: Beraneck M. , McKee J. L. , Aleisa M. , Cullen K. E.
Published: May 2008

The term “vestibular compensation” refers to the resolution of motor deficits resulting from a peripheral vestibular lesion. We investigated the role of the cerebellum in the compensation process by characterizing the vestibuloocular reflex (VOR) evoked by head rotations at frequencies and velocities similar to those in natural behaviors in wild-type ( WT) versus cerebellar-deficient Lurcher ( Lc/+) mice. We found that during exploratory activity, normal mice produce head rotations largely consisting of frequencies ≤4 Hz and velocities and accelerations as large as 400°/s and 5,000°/s2, respectively. Accordingly, the VOR was characterized using sinusoidal rotations (0.2–4 Hz) as well as transient impulses (∼400°/s; ∼2,000°/s2). Before lesions, WT and Lc/+ mice produced similar VOR responses to sinusoidal rotation. Lc/+ mice, however, had significantly reduced gains for transient stimuli. After unilateral labyrinthectomy, VOR recovery followed a similar course for WT and Lc/+ groups during the first week: gain was reduced by 80% for ipsilesionally directed head rotations on day 1 and improved for both strains to values of ∼0.4 by day 5. Moreover, responses evoked by contralesionally directed rotations returned to prelesion in both strains within this period. However, unlike WT, which showed improving responses to ipsilesionally directed rotations, recovery plateaued after first week for Lc/+ mice. Our results show that despite nearly normal recovery in the acute phase, long-term compensation is compromised in Lc/+. We conclude that cerebellar pathways are critical for long-term restoration of VOR during head rotation toward the lesioned side, while noncerebellar pathways are sufficient to restore proper gaze stabilization during contralesionally directed movements.
Silencing-Induced Metaplasticity in Hippocampal Cultured Neurons
Authors: Sokolova Irina V. , Mody Istvan
Published: May 2008

Silencing-induced homeostatic plasticity is usually expressed as a change in the amplitude or the frequency of miniature postsynaptic currents. Here we report that, prolonged (∼24 h) silencing of mature (20–22 days in vitro) cultured hippocampal neurons using the voltage-gated sodium channel blocker tetrodotoxin (TTX) produced no effects on the amplitude or frequency of the miniature excitatory postsynaptic currents (mEPSCs). However, the silencing changed the intrinsic membrane properties of the neurons, resulting in an increased excitability and rate of action potentials firing upon TTX washout. Allowing neurons to recover in TTX-free recording solution for a short period of time after the silencing resulted in potentiation of mEPSC amplitudes. This form of activity-dependent potentiation is different from classical long-term potentiation, as similar potentiation was not seen in nonsilenced neurons treated with bicuculline to raise their spiking activity to the same level displayed by the silenced neurons during TTX washout. Also, the potentiation of mEPSC amplitudes after the recovery period was not affected by the N-methyl-d-aspartate receptor blocker d-2-amino-5-phosponopentanoic acid or by the calcium/calmodulin-dependent kinase II (CaMKII) inhibitor KN-62 but was abolished by the L-type calcium channel blocker nifedipine. We thus conclude that the potentiation of mEPSC amplitudes following brief recovery of spiking activity in chronically silenced neurons represents a novel form of metaplasticity that differs from the conventional models of homeostatic synaptic plasticity.