bluuebunny/crossref_metadata_2025 huggingface dataset:
Using deep DenseNet with cyclical learning rate to classify leukocytes for leukemia identification
Authors: Houssein Essam H. , Mohamed Osama , Abdel Samee Nagwan , Mahmoud Noha F. , Talaat Rawan , Al-Hejri Aymen M. , Al-Tam Riyadh M.
Published: September 2023

Background

The examination, counting, and classification of white blood cells (WBCs), also known as leukocytes, are essential processes in the diagnosis of many disorders, including leukemia, a kind of blood cancer characterized by the uncontrolled proliferation of carcinogenic leukocytes in the marrow of the bone. Blood smears can be chemically or microscopically studied to better understand hematological diseases and blood disorders. Detecting, identifying, and categorizing the many blood cell types are essential for disease diagnosis and therapy planning. A theoretical and practical issue. However, methods based on deep learning (DL) have greatly helped blood cell classification.

Materials and Methods

Images of blood cells in a microscopic smear were collected from GitHub, a public source that uses the MIT license. An end-to-end computer-aided diagnosis (CAD) system for leukocytes has been created and implemented as part of this study. The introduced system comprises image preprocessing and enhancement, image segmentation, feature extraction and selection, and WBC classification. By combining the DenseNet-161 and the cyclical learning rate (CLR), we contribute an approach that speeds up hyperparameter optimization. We also offer the one-cycle technique to rapidly optimize all hyperparameters of DL models to boost training performance.

Results

The dataset has been split into two sets: approximately 80% of the data (9,966 images) for the training set and 20% (2,487 images) for the validation set. The validation set has 623, 620, 620, and 624 eosinophil, lymphocyte, monocyte, and neutrophil images, whereas the training set has 2,497, 2,483, 2,487, and 2,499, respectively. The suggested method has 100% accuracy on the training set of images and 99.8% accuracy on the testing set.

Conclusion

Using a combination of the recently developed pretrained convolutional neural network (CNN), DenseNet, and the one fit cycle policy, this study describes a technique of training for the classification of WBCs for leukemia detection. The proposed method is more accurate compared to the state of the art.
Case Report: Mycosis fungoides as an exclusive manifestation of common variable immunodeficiency in a family with a NFKB2 gene mutation
Authors: Spangenberg María Noel , Grille Sofía , Simoes Camila , Brandes Mariana , Garcia-Luna Joaquín , Catalán Ana Inés , Ranero Sabrina , Boada Matilde , Brugnini Andreína , Trias Natalia , Lens Daniela , Raggio Víctor , Spangenberg Lucía
Published: September 2023

Background

Common variable immunodeficiency disorders (CVIDs), which are primary immunodeficiencies characterized by the failure of primary antibody production, typically present with recurrent bacterial infections, decreased antibody levels, autoimmune features, and rare atypical manifestations that can complicate diagnosis and management. Although most cases are sporadic, approximately 10% of the patients may have a family history of immunodeficiency. Genetic causes involving genes related to B-cell development and survival have been identified in only a small percentage of cases.

Case presentation

We present the case of a family with two brothers who presented with mycosis fungoides as an exclusive symptom of a common variable immunodeficiency disorder (CVID). Whole-exome sequencing of the index patient revealed a pathogenic variant of the NFKB2 gene. Based on this diagnosis and re-evaluation of other family members, the father and brother were diagnosed with this rare immune and preneoplastic syndrome. All CVID-affected family members presented with mycosis fungoides as their only symptom, which is, to the best of our knowledge, the first case to be reported.

Conclusion

This case highlights the importance of high-throughput sequencing techniques for the proper diagnosis and treatment of hereditary hematological disorders.
Fertility preservation and monitoring in adult patients diagnosed with lymphoma: consensus-based practical recommendations by the Fondazione Italiana Linfomi & Società Italiana della Riproduzione Umana
Authors: Minoia Carla , Viviani Simonetta , Silvestris Erica , Palini Simone , Parissone Francesca , De Palma Giuseppe , Fedina Anna , Cormio Gennaro , Guarini Attilio , Gini Guido , Montano Luigi , Merli Francesco , Peccatori Fedro Alessandro
Published: September 2023

Introduction

Fertility preservation (FP) and monitoring has considerable relevance in the multidisciplinary approach to cancer patients. In these consensus-based practical recommendations, the scientific societies Fondazione Italiana Linfomi (FIL) and Società Italiana della Riproduzione Umana (SIRU) reviewed the main aspects and identified the optimal paths which aim to preserve and monitor fertility in patients diagnosed with lymphoma at the different phases of the disease and during long-term survivorship.

Methods

For the Panel, eleven experts were selected for their expertise in research and clinical practice on onco-fertility and lymphoma. The Panel’s activity was supervised by a chairman. A series of rank-ordering key questions were proposed according to their clinical relevance and discussed among the Panel, focusing on patients diagnosed with non-Hodgkin’s lymphomas and Hodgkin lymphoma. Agreement among all the Panelists on the content and terminology of the statements was evaluated by a web-based questionnaire according to the Delphi methodology.

Results

From the literature review a total of 78 questions or sentences, divided into the 6 areas of interest, were identified. By applying the Gwet's AC, k was: Section 1: 0,934 (Very good); Section 2: 0,958 (Very good); Section 3: 0,863 (Very good); Section 4: 0,649 (Good); Section 5: 0,936 (Very good); Section 6 raw agreement 100%. Two rounds of Delphi allowed to provide the maximum agreement. All statements were newly discussed in a round robin way and confirmed for the drafting of the final recommendations.

Discussion

These recommendations would be useful for onco-hematologists, gynecologists, urologists, and general practice physicians who take care of young lymphoma patients to guarantee an evidence-based oncofertility assessment and treatment during the oncologic pathway.
Quantitative sensory testing in a magnetic resonance environment: considerations for thermal sensitivity and patient safety
Authors: Kim Ayeong (Jenny) , Szabo Edina , Lunde Claire E. , Comptdaer Gabriela , Zurakowski David , Sieberg Christine B. , Holmes Scott A.
Published: September 2023

Introduction

Quantitative sensory testing (QST) is often used to understand the perceptual basis of acute and chronic conditions, including pain. As the need grows for developing a mechanistic understanding of neurological pathways underlying perception in the basic and clinical sciences, there is a greater need to adapt techniques such as QST to the magnetic resonance (MR) environment. No studies have yet evaluated the impact of the MR environment on the perception of thermal stimuli. This study aimed to evaluate the differences in temperature sensitivity outside an MR environment and during an MRI scanning session. We hypothesized that there would be a difference in how participants reported their pain sensitivity between the two environments.

Methods

Healthy participants underwent thermal QST outside the MR scanning environment, where they were asked to rate the temperature of a noxious stimulus at which they perceived their pain to be 7/10, using a Likert scale ranging from 0 to 10. Participants repeated this procedure inside a 3.0 T MRI approximately 30 min later. We repeated our investigation in a clinical cohort of participants with a chronic pain condition.

Results

There were statistically significant changes of 1.1°C in thermal sensitivity between environments. This increase in pain threshold was found in healthy participants and replicated in the clinical cohort.

Discussion

Findings can be applied toward improving MR safety, the resolution of brain pathways underlying pain mechanisms, and to more broadly comment on the impact of the MR environment on investigations that integrate perception-influenced processes.
Chronic pain: its impact on the quality of life and gender
Authors: Pandelani Funeka Faith , Nyalunga Suzan Louisa Nnanile , Mogotsi Miriam Morongwa , Mkhatshwa Vangile Bridget
Published: September 2023

Background

Chronic pain poses a considerable challenge to individuals' well-being, leading to decreased quality of life, limitations in daily functioning, and a higher reliance on healthcare services, resulting in significant economic burdens. In South Africa, chronic pain ranks among the prevalent chronic health conditions, although the exact prevalence might differ across different regions. To address this issue effectively, it is crucial to gain a comprehensive understanding of the problem by utilising the most up-to-date and relevant data available.

Aim

The aim of this study was to assess the impact of chronic pain on the quality of life and gender of the patients attending a primary healthcare centre.

Methods

We conducted a cross-sectional quantitative study among chronic care patients at Soshanguve Community Health Centre (CHC). The study utilized a validated Wisconsin Brief Pain Questionnaire to collect data. A total of 331 patients actively participated in the study.

Results

The prevalence of chronic pain was 21.5% [95% CI: 17.0–25.9]. Females were affected more frequently than male patients, chronic pain was 11.1% greater in females than in male. Furthermore, chronic pain mildly impacted the general activity of patients 33.8% [95% CI: 23.9–45.4], mood 42.3% [ 95% CI: 31.4–53.8], walking ability 29.6% [95% CI: 20.2–41.0], relationships 47.9% [95% CI: 36.7–59.3), sleep 31.0% [95% CI: 21.4–42.5], enjoyment of life 39.4% [95% CI: 28.9–51.1] and normal working ability 25.3% [ 95% CI: 16.7–36.6].

Conclusions

The exact Fisher test conducted to assess the association between the experienced chronic pain and its impact on the quality of life yielded a significant result, with a p-value of 0.0071 (p < 0.05). This indicates that a considerable number of patients are currently enduring chronic pain that has a noticeable effect on their overall quality of life. These findings offer invaluable insights that are essential for enhancing resource allocation at the primary care level and facilitating a more comprehensive evaluation of pain management in our communities.
Low levels of serum vitamin C in children with limb fractures: a case-control study
Authors: Ding Yangkun , Wang Chunhua , Yu Jiazhi , Lu Mingzhu , Mu Pengfei , Liu Xiangfei , Liu Tao
Published: September 2023

Introduction

The role of vitamin C in pediatric fractures has not received much attention, although it is known to be a factor in osteoporotic fractures in the elderly. This case-control study aimed to investigate the changes in serum vitamin C levels among children with limb fractures.

Methods

We recruited 325 children with and 316 children without limb fractures hospitalized between January 2021 and December 2021. Following admission, basic demographic data of all participants were collected, and fasting serum vitamin C levels were determined using ultra-high-performance liquid chromatography-tandem mass spectrometry.

Results

The mean age of the fracture group was 5.1 years (95% CI, 4.83–5.33). The serum vitamin C levels in the fracture group (4.48 µg/ml) were significantly lower than those in the control group (8.38 µg/ml) (p < 0.0001). Further subgroup analysis of the fracture group revealed that serum vitamin C levels decreased significantly after 4 years of age and there was a significant difference in the duration after injury between <6 and >6 h (p = 0.0224). Spearman’s rank correlation coefficient suggested that age and vitamin C levels were negatively correlated in the fracture group.

Conclusion

In general, children with limb fractures had lower serum vitamin C levels, especially those aged 4 years and over.
Pulmonary mucoepidermoid carcinoma in children: two case reports and a review of the literature
Authors: Huang Yuan , Fu Yong , Sun Jing , Xu Bin , Wu Lei , Tang Lan-fang
Published: September 2023

Pulmonary mucoepidermoid carcinoma (PMEC) is a rare tumor, particularly in children, and its clinical manifestations vary. When the tumor is small, it may be asymptomatic; however, with larger tumors, patients may present with symptoms such as recurring pneumonia, atelectasis, persistent cough, chest pain, and even hemoptysis. PMEC appears as an exophytic intrabronchial mass. This study aims to report on the clinical manifestations, imaging findings, treatment approaches, and prognosis of two children diagnosed with PMEC at our hospital between January 2018 and December 2022. The age of onset for both children was 9 years, and the masses were located in the right upper lobe bronchi. Following surgical treatment, both patients showed a good prognosis. In addition, we conducted a comprehensive review of the relevant literature to enhance the overall understanding of PMEC.
Improving NICU staff decision-making with parents in medical rounds: a pilot study of reflective group dialogue intervention
Authors: Ahlqvist-Björkroth Sari , Thernström Blomqvist Ylva , Nyberg Jenni , Normann Erik , Axelin Anna
Published: September 2023

Introduction

The communication skills of healthcare professionals play a crucial role in successful shared decision-making with parents in neonatal intensive care. Improving communication skills can be achieved through practice and reflection on personal experiences after authentic interaction events with parents. The process of reflection typically involves three phases: description, reflection, and critical reflection. In this study, our aim was to explore the acceptability of the Reflective Group Dialogue intervention and its effectiveness in supporting the reflective process.

Methods

This qualitative pilot study was conducted in the neonatal intensive care unit at Uppsala University Children's Hospital, Sweden. The sample consisted of nine medical rounds with seven families, five neonatologists, seven registered nurses, and five assistant nurses. Purposive sampling was used to collect the data. The intervention comprised four elements: (1) before the intervention, a recorded presentation on shared decision-making was given to the entire unit staff, (2) an observation of a normal medical round discussion with parents, (3) an interview with parents about their experience after the same round, and (4) a reflective discussion with the participating health care professionals after the round. The parent interviews and reflective discussions were audio-recorded and transcribed verbatim. They were analyzed using thematic analysis as a theoretical strategy.

Results

Both parents and staff widely accepted the intervention and found it beneficial. We identified four discussions that remained in the descriptive phase of the reflection process, four that reached the reflective phase, and one that reached the critical reflection phase. The descriptive discussions were characterized by using a single perspective to reflect, often based on personal opinions. The reflective discussions included analyzing interaction sequences from both staff and parent perspectives and were primarily based on actual observations of communication during medical rounds. The critical discussion led to a new awareness of current practices concerning parental involvement in decision-making. These discussions also utilized “what-if” thinking to evaluate potential new practices and their pros and cons.

Conclusions

The intervention seems promising as it was perceived as beneficial by the recipients and facilitated reflection in most cases. However, to enhance the feasibility of the intervention, some improvements are discussed.
Glucagon-like peptide 1 (GLP-1) receptor agonists in experimental Alzheimer’s disease models: a systematic review and meta-analysis of preclinical studies
Authors: Kong Fanjing , Wu Tianyu , Dai Jingyi , Zhai Zhenwei , Cai Jie , Zhu Zhishan , Xu Ying , Sun Tao
Published: September 2023

Alzheimer’s disease (AD) is a degenerative disease of the nervous system. Glucagon-like peptide-1 receptor agonists (GLP-1 RAs), a drug used to treat type 2 diabetes, have been shown to have neuroprotective effects. This systematic review and meta-analysis evaluated the effects and potential mechanisms of GLP-1 RAs in AD animal models. 26 studies were included by searching relevant studies from seven databases according to a predefined search strategy and inclusion criteria. Methodological quality was assessed using SYRCLE’s risk of bias tool, and statistical analysis was performed using ReviewManger 5.3. The results showed that, in terms of behavioral tests, GLP-1 RAs could improve the learning and memory abilities of AD rodents; in terms of pathology, GLP-1 RAs could reduce Aβ deposition and phosphorylated tau levels in the brains of AD rodents. The therapeutic potential of GLP-1 RAs in AD involves a range of mechanisms that work synergistically to enhance the alleviation of various pathological manifestations associated with the condition. A total of five clinical trials were retrieved fromClinicalTrials.gov. More large-scale and high-quality preclinical trials should be conducted to more accurately assess the therapeutic effects of GLP-1 RAs on AD.
Properties of the particle distribution in Pb–Pb collisions at sNN=5.02 TeV and sNN=2.76 TeV
Authors: Geng Yan-Feng , Li Bao-Chun
Published: September 2023

Properties of the particle distribution in high energy heavy-ion collisions are important for understanding the particle production. In Tsallis statistics with a multisource production, we study the transverse momentum spectra of D0, D+, D*+, Ds+, J/ψ in Pb–Pb collisions at sNN = 5.02 TeV and charged particles in Pb–Pb collisions at sNN = 2.76 TeV. A good agreement can be observed between the results obtained in the model and the experimental results of ALICE and CMS collaboration. The nuclear modification factor RAA is reproduced. Properties reflected in the multiparticle system are discussed by the parameters provided in the improved model. It is found that the temperature T and the tft/τ increase with the centrality for the same particle due to the excitation degree of the multiparticle system. The non-equilibrium degree at sNN = 5.02 TeV is larger than that at sNN = 2.76 TeV. It shows that the system at the larger collision energy deviates farther from the equilibrium state.
Probiotic effects of Lactococcus lactis and Leuconostoc mesenteroides on stress and longevity in Caenorhabditis elegans
Authors: Stover Mylissa A. , Tinoco-Bravo Brenda , Shults Crystal A. , Marouk Sydney , Deole Ratnakar , Manjarrez Jacob R.
Published: September 2023

The short lifespan of Caenorhabditis elegans enables the efficient investigation of probiotic interventions affecting stress and longevity involving the potential therapeutic value of Lactococcus lactis and Leuconostoc mesenteroides isolated from organic basil. The lactic acid bacteria were cultured from the produce collected from a local grocery store in Tulsa, Oklahoma, and then identified through 16S rDNA sequencing and biochemical tests. To dive deep into this analysis for potential probiotic therapy, we used fluorescent reporters that allow us to assess the differential induction of multiple stress pathways such as oxidative stress and the cytoplasmic, endoplasmic reticulum, and the mitochondrial unfolded protein response. This is combined with the classic health span measurements of survival, development, and fecundity, allowing a wide range of organismal observations of the different communities of microbes supported by probiotic supplementation with Lactococcus lactis and Leuconostoc mesenteroides. These strains were initially assessed in relation to the Escherichia coli feeding strain OP50 and the C. elegans microbiome. The supplementation showed a reduction in the median lifespan of the worms colonized within the microbiome. This was unsurprising, as negative results are common when probiotics are introduced into healthy microbiomes. To further assess the supplementation potential of these strains on an unhealthy (undifferentiated) microbiome, the typical axenic C. elegans diet, OP50, was used to simulate this single-species biome. The addition of lactic acid bacteria to OP50 led to a significant improvement in the median and overall survival in simulated biomes, indicating their potential in probiotic therapy. The study analyzed the supplemented cultures in terms of C. elegans’ morphology, locomotor behavior, reproduction, and stress responses, revealing unique characteristics and stress response patterns for each group. As the microbiome’s influence on the health span gains interest, the study aims to understand the microbiome relationships that result in differential stress resistance and lifespans by supplementing microbiomes with Lactococcus lactis and Leuconostoc mesenteroides isolated from organic basil in C. elegans.
Angiotensinergic and GABAergic transmission in the medial preoptic area: role in urinary bladder and cardiovascular control in female rats
Authors: Daiuto Sergio A. , de Carvalho Rodrigo P. , Vale Bárbara do , Dsouki Nuha A. , Giannocco Gisele , Cafarchio Eduardo M. , Aronsson Patrik , Sato Monica A.
Published: September 2023

Introduction: The medial preoptic area (mPOA) participates in thermoregulatory control and blood pressure modulation as shown by studies with electrical stimulation of this area or cobalt chloride injection, a non-selective synapse inhibitor. This study aimed to investigate whether angiotensin II (Ang II) and GABA could act or not in the mPOA to mediate the cardiovascular and micturition control pathways.

Methods: Female Wistar rats were submitted to stereotaxic surgery for implantation of a guide cannula into the mPOA 7 days prior to the experiments. Afterwards, the animals were isoflurane- anesthetized and submitted to the catheterization of the femoral artery and vein and urinary bladder cannulation for mean arterial pressure (MAP), heart rate (HR), and intravesical pressure (IP) recordings, respectively. After the baseline MAP, HR, and IP recordings for 15 min, Ang II (0.1 nM, 1 μL), losartan (AT-1 receptor antagonist, 100 nM, 1 μL), GABA (50 mM, 1 μL) or saline (1 μL) were injected into the mPOA, and the variables were measured for additional 30 min. In a different group of rats, the AT-1 receptor, angiotensin II converting enzyme (ACE), and GABAa receptor gene expression was evaluated in mPOA samples by qPCR. The data are as mean ± SEM and submitted to One-way ANOVA (Tukey posttest) or paired Student t-test (P <0.05).

Results: The injection of Ang II into the mPOA evoked a significant hypotension (−37±10 mmHg, n = 6, p = 0.024) and bradycardia (−47 ± 20 bpm, p = 0.030) compared to saline (+1 ± 1 mmHg and +6 ± 2 bpm, n = 6). A significant increase in IP was observed after Ang II injection into the mPOA (+72.25 ± 17.91%, p = 0.015 vs. −1.80 ± 2.98%, n = 6, saline). No significant changes were observed in MAP, HR and IP after the losartan injection in the mPOA compared to saline injection. Injection of GABA into the mPOA evoked a significant fall in MAP and HR (−68 ± 2 mmHg, n = 6, p < 0.0001 and −115 ± 14 bpm, n = 6, p = 0.0002 vs. −1 ± 1 mmHg and +4 ± 2 bpm, n = 6, saline), but no significant changes were observed in IP. The AT-1 receptor, ACE and GABAa receptor mRNA expression was observed in all mPOA samples.

Discussion: Therefore, in female rats, Ang II mediated transmission in the mPOA is involved in the cardiovascular regulation and in the control of central micturition pathways. A phasic control dependent on AT-1 receptors in the mPOA seems to be involved in the regulation of those cardiovascular and intravesical 3 parameters. In contrast, GABAergic transmission in the mPOA participates in the pathways of cardiovascular control in anesthetized female rats, nevertheless, this neurotransmission is not involved in the micturition control.
p90RSK2, a new MLCK mediates contractility in myosin light chain kinase null smooth muscle
Authors: Kalra Jaspreet , Artamonov Mykhaylo , Wang Hua , Franke Aaron , Markowska Zaneta , Jin Li , Derewenda Zygmunt S. , Ayon Ramon J. , Somlyo Avril
Published: September 2023

Introduction: Phosphorylation of smooth muscle (SM) myosin regulatory light chain (RLC20) is a critical switch leading to SM contraction. The canonical view held that only the short isoform of myosin light chain kinase (MLCK1) catalyzed this reaction. It is now accepted that auxiliary kinases may contribute to vascular SM tone and contractility. We have previously reported that p90 ribosomal S6 kinase (RSK2) functions as such a kinase, in parallel with MLCK1, contributing ∼25% of the maximal myogenic force in resistance arteries. Thus, RSK2 may be instrumental in the regulation of basal vascular tone and blood pressure. Here, we take advantage of a MLCK1 null mouse (mylk1−/−) to further test our hypothesis that RSK2 can function as an MLCK, playing a significant physiological role in SM contractility.

Methods: Using fetal (E14.5-18.5) SM tissues, as embryos die at birth, we investigated the necessity of MLCK for contractility and fetal development and determined the ability of RSK2 kinase to compensate for the lack of MLCK and characterized its signaling pathway in SM.

Results and Discussion: Agonists induced contraction and RLC20 phosphorylation in mylk1−/− SM was attenuated by RSK2 inhibition. The pCa-tension relationships in permeabilized strips of bladder showed no difference in Ca2+ sensitivity in WT vs mylk1−/− muscles, although the magnitude of force responses was considerably smaller in the absence of MLCK. The magnitude of contractile responses was similar upon addition of GTPγS to activate the RhoA/ROCK pathway or calyculinA to inhibit the myosin phosphatase. The Ca2+-dependent tyrosine kinase, Pyk2, contributed to RSK2-mediated contractility and RLC20 phosphorylation. Proximity-ligation and immunoprecipitation assays demonstrated an association of RSK2, PDK1 and ERK1/2 with MLCK and actin. RSK2, PDK1, ERK1/2 and MLCK formed a signaling complex on the actin filament, positioning them for interaction with adjacent myosin heads. The Ca2+-dependent component reflected the agonist mediated increases in Ca2+, which activated the Pyk2/PDK1/RSK2 signaling cascade. The Ca2+−independent component was through activation of Erk1/2/PDK1/RSK2 leading to direct phosphorylation of RLC20, to increase contraction. Overall, RSK2 signaling constitutes a new third signaling pathway, in addition to the established Ca2+/CaM/MLCK and RhoA/ROCK pathways to regulate SM contractility.
Therapeutic role of exosomes and conditioned medium in keloid and hypertrophic scar and possible mechanisms
Authors: Zhong Yixiu , Zhang Youfan , Yu Aijiao , Zhang Zhiwen , Deng Zhenjun , Xiong Kaifen , Wang Qi , Zhang Jianglin
Published: September 2023

Exosomes, ranging from 40 to 160 nm in diameter, are extracellular lipid bilayer microvesicles that regulate the body’s physiological and pathological processes and are secreted by cells that contain proteins, nucleic acids, amino acids and other metabolites. Previous studies suggested that mesenchymal stem cell (MSC)-derived exosomes could either suppress or support keloid and hypertrophic scar progression. Although previous research has identified the potential value of MSC-exosomes in keloid and hypertrophic scar, a comprehensive analysis of different sources of MSC-exosome in keloid and hypertrophic scar is still lacking. This review mainly discusses different insights regarding the roles of MSC-exosomes in keloid and hypertrophic scar treatment and summarizes possible underlying mechanisms.
Analysis of oxygen consumption rates in zebrafish reveals differences based on sex, age and physical activity recovery
Authors: Konadu Bridget , Hosler Jonathan P. , Gibert Yann , Edwards Kristin S.
Published: September 2023

Introduction: Mitochondrial dysfunction is linked to a variety of human diseases. Understanding the dynamic alterations in mitochondrial respiration at various stages of development is important to our understanding of disease progression. Zebrafish provide a system for investigating mitochondrial function and alterations during different life stages. The purpose of this study was to investigate our ability to measure mitochondrial oxygen consumption rates in zebrafish embryos, larvae, and adults as an indicator of mitochondrial function.

Methods: Basal respiration of entire zebrafish embryos (5 dpf), larvae (0.6–0.9 cm), young adults (3-month-old), and old adults (12-month-old) was measured using an Oroboros Oxygraph, with a stirrer speed of 26 rpm. For embryos and larvae, “leak” respiration (plus oligomycin), maximum respiration (plus uncoupler), non-mitochondrial respiration (plus inhibitors), and complex IV activity were also measured. To induce physical activity in adult fish, the stirrer speed was increased to 200 rpm.

Results and Discussion: We demonstrate the ability to accurately measure respiration rates in zebrafish at various ages using the Oroboros Oxygraph. When comparing zebrafish embryos to larvae, embryos have a higher maximum respiration. Three-month-old zebrafish males have higher basal respiration than females, while 12-month-old zebrafish females exhibit greater rates of respiration than males and younger females. When the stirrer speed was increased, respiration rates decrease, but with differences depending on sex. This study demonstrates a simple and accessible method to assess zebrafish physiology by mitochondrial oxygen consumption measurements in an unmodified Oroboros Oxygraph. The method should facilitate studies to understand the intricate interplay between mitochondrial function, development, and aging.
Image-based phenotyping of seed architectural traits and prediction of seed weight using machine learning models in soybean
Authors: Duc Nguyen Trung , Ramlal Ayyagari , Rajendran Ambika , Raju Dhandapani , Lal S. K. , Kumar Sudhir , Sahoo Rabi Narayan , Chinnusamy Viswanathan
Published: September 2023

Among seed attributes, weight is one of the main factors determining the soybean harvest index. Recently, the focus of soybean breeding has shifted to improving seed size and weight for crop optimization in terms of seed and oil yield. With recent technological advancements, there is an increasing application of imaging sensors that provide simple, real-time, non-destructive, and inexpensive image data for rapid image-based prediction of seed traits in plant breeding programs. The present work is related to digital image analysis of seed traits for the prediction of hundred-seed weight (HSW) in soybean. The image-based seed architectural traits (i-traits) measured were area size (AS), perimeter length (PL), length (L), width (W), length-to-width ratio (LWR), intersection of length and width (IS), seed circularity (CS), and distance between IS and CG (DS). The phenotypic investigation revealed significant genetic variability among 164 soybean genotypes for both i-traits and manually measured seed weight. Seven popular machine learning (ML) algorithms, namely Simple Linear Regression (SLR), Multiple Linear Regression (MLR), Random Forest (RF), Support Vector Regression (SVR), LASSO Regression (LR), Ridge Regression (RR), and Elastic Net Regression (EN), were used to create models that can predict the weight of soybean seeds based on the image-based novel features derived from the Red-Green-Blue (RGB)/visual image. Among the models, random forest and multiple linear regression models that use multiple explanatory variables related to seed size traits (AS, L, W, and DS) were identified as the best models for predicting seed weight with the highest prediction accuracy (coefficient of determination, R2=0.98 and 0.94, respectively) and the lowest prediction error, i.e., root mean square error (RMSE) and mean absolute error (MAE). Finally, principal components analysis (PCA) and a hierarchical clustering approach were used to identify IC538070 as a superior genotype with a larger seed size and weight. The identified donors/traits can potentially be used in soybean improvement programs
Bamboo expansion promotes radial growth of surviving trees in a broadleaf forest
Authors: Gong Chao , Zeng Xiaoxia , Zhu Xianglong , Huang Wenhui , Compson Zacchaeus G. , Ren Zewen , Ran Huan , Song Qingni , Yang Qingpei , Huang Dongmei , Liu Jun
Published: September 2023

Introduction

Considerable evidence indicates that some trees are more vulnerable than others during bamboo (Phyllostachys edulis) expansion, which can affect plant community structure and alter the environment, but there has been insufficient research on the growth status of surviving individuals in colonized forests.

Methods

In this study, we compared the annual growth increment, growth rate, and onset, cessation, and duration of radial growth of Alniphyllum fortunei, Machilus pauhoi, and Castanopsis eyrei in a bamboo-expended broadleaf forest (BEBF) and a bamboo-absent broadleaf forest (BABF) using high-resolution point dendrometers.

Results

We found that the annual radial growth of A. fortunei, M. pauhoi, and C. eyrei was 22.5%, 172.2%, and 59.3% greater in BEBF than in BABF, respectively. The growth rates of M. pauhoi and C. eyrei in BEBF were significantly higher than in BABF by13.9 μm/d and 19.6 μm/d, whereas A. fortunei decreased significantly by 7.9 μm/d from BABF to BEBF. The onset and cessation of broad-leaf tree growth was later, and the growth duration was longer in BEBF compared to BABF. For example, A. fortunei and M. pauhoi in BEBF had more than one month longer growth duration than in BABF. Additionally, the nighttime growth rates of some surviving broad-leaf trees in BEBF was significantly higher than that in BABF.

Discussion

These results suggest that the surviving trees have plasticity and can adapt to atmospheric changes and competitive relationships after expansion of bamboo in one of two ways: by increasing their growth rates or by modifying onset and cessation of growth to extend the growth duration of trees or avoid the period of intense competition with bamboo, thereby growing better. Our research reveals for the first time how the growth of surviving broad-leaf trees adjusts to bamboo expansion. These results provide insights into how biological expansions impact primary production and have implications for forest management in the Anthropocene.
BvCPD promotes parenchyma cell and vascular bundle development in sugar beet (Beta vulgaris L.) taproot
Authors: Guo Xiaotong , Li Yue , Li Ningning , Li Guolong , Sun Yaqing , Zhang Shaoying
Published: September 2023

Constitutive photomorpogenic dwarf (CPD) is a pivotal enzyme gene for brassinolide (BR) synthesis and plays an important role in plant growth, including increasing plant biomass and plant height, elongating cells, and promoting xylem differentiation. However, little is known about the function of the CPD gene in sugar beet. In the current study, we isolated CPD from Beta vulgaris L. (BvCPD), which encodes protein localized in the nucleus, cell membrane, and cell wall. BvCPD was strongly expressed in parenchyma cells and vascular bundles. The transgenic sugar beet overexpressing BvCPD exhibited larger diameter than that of the wild type (WT), which mainly owing to the increased number and size of parenchyma cells, the enlarged lumen and area of vessel in the xylem. Additionally, overexpression of BvCPD increased the synthesis of endogenous BR, causing changes in the content of endogenous auxin (IAA) and gibberellin (GA) and accumulation of cellulose and lignin in cambium 1–4 rings of the taproot. These results suggest that BvCPD can promote the biosynthesis of endogenous BR, improve cell wall components, promote the development of parenchyma cells and vascular bundle, thereby playing an important role in promoting the growth and development of sugar beet taproot.
Beyond polarization towards dynamic balance: harmony as the core of mental health
Authors: Delle Fave Antonella , Wissing Marié Philipina , Brdar Ingrid
Published: September 2023

Worldviews are culturally derived assumptions that influence individual and collective behaviors, values, and representations of reality. The study of mental functions is not exempt from this influence, as reflected in scientific theories, methodological approaches, and empirical studies. Despite acknowledging the interplay of mental processes with developmental, environmental, and cultural dimensions, psychological research is still primarily based on quantitative methods, and on the conceptualization of mental phenomena as unfolding along polarized continua. A lively epistemological debate surrounds this approach, especially underscoring the risk of blurring the distinction between constructs derived from statistical models and real-life processes and experiences. Based on this debate and on recent empirical evidence derived from the positive psychology literature, this paper is aimed at proposing an integrated view of mental health, as a holistically patterned, contextually imbedded, and dynamic phenomenon changing over time and across life events, with harmony, harmonization and dynamic balance as core qualities. The heuristic potential of investigating the qualitative configuration patterns of mental health dimensions across individuals and groups, beyond their position along a quantitative continuum, is outlined. The development of more integrated approaches and methodologies to investigate mental health as a harmonization process, taking into account personal, contextual and developmental features, would be aligned with evidence derived from the integration of traditional nomothetic and ideographic approaches, and other life sciences. However, the development of a transdisciplinary line of research requires further inputs from different epistemological views, as well as higher attention to the potential contribution of different philosophical traditions.
Discursive delegitimisation of homosexuality on Chinese social media
Authors: Zhang Ke , Zhuang Huibin
Published: September 2023

This article contributes to the understanding of public representations of homosexuality in China by focusing on the case of a homophobic textbook. College student Xixi sued Jinan University Press (JUP) in 2017 for classifying homosexuality as a psychosexual disorder. Three years later, a Chinese court dismissed Xixi’s lawsuit against the allegedly homophobic textbook published by JUP. The ruling elicited responses on Chinese social media that demonstrated the polarisation of public opinion regarding homosexuality. This article investigates discursive representations of homosexuality in online space by analysing the public discourse surrounding this problem. Using van Leeuwen’s discursive delegitimation strategies (i.e., authorisation, moral evaluation, rationalisation, and mythopoesis), 496 comments posted on Sina Weibo were employed and subjected to discourse analysis. According to our findings, these strategies contribute to public opposition to homosexuality, portrayed as unhealthy, infertile, disruptive, and corrosive. The article concludes by discussing the emerging sociocultural factors on Sina Weibo that influence the anti-homosexuality attitudes of Weibo users.
Eye movement corpora in Adyghe and Russian: an eye-tracking study of sentence reading in bilinguals
Authors: Zdorova Nina , Parshina Olga , Ogly Bela , Bagirokova Irina , Krasikova Ekaterina , Ziubanova Anastasiia , Unarokova Shamset , Makerova Susanna , Dragoy Olga
Published: September 2023

The present study expands the eye-tracking-while reading research toward less studied languages of different typological classes (polysynthetic Adyghe vs. synthetic Russian) that use a Cyrillic script. In the corpus reading data from the two languages, we confirmed the widely studied effects of word frequency and word length on eye movements in Adyghe-Russian bilingual individuals for both languages. We also confirmed morphological effects in Adyghe reading (part-of-speech class and the number of lexical affixes) that were previously shown in some morphologically-rich languages. Importantly, we demonstrated that bilinguals’ reading in Adyghe does differ quantitatively (the effect of language on reading times) and qualitatively (different effects of landing and previous/upcoming words on the eye movements within a current word) from their reading in Russian.
Self-reported traumatic brain injury in a sample of impulsive violent offenders: neuropsychiatric correlates and possible “dose effects”
Authors: Challakere Ramaswamy Vasudeva Murthy , Butler Tony , Ton Bianca , Wilhelm Kay , Mitchell Philip B. , Knight Lee , Greenberg David , Ellis Andrew , Allnutt Stephen , Jones Jocelyn , Gebski Val , Carr Vaughan , Scott Rodney J. , Schofield Peter William
Published: September 2023

Background

Traumatic brain injury (TBI) is a major public health problem that may be associated with numerous behavioral problems, including impulsivity, aggression and violence. Rates of self-reported TBI are high within offender populations, but the extent to which TBI is causally implicated in causing illegal behavior is unclear. This study examined the psychological and functional correlates of histories of traumatic brain injury in a sample of impulsive violent offenders.

Methods

Study participants, all men, had been recruited to participate in a randomized controlled trial of sertraline to reduce recidivism. Study entry criteria were an age of at least 18 years, a documented history of two or more violent offenses and a score of 70 or above on the Barratt Impulsiveness Scale. An extensive list of standardized questionnaires was administered to obtain information on previous TBI and other neuropsychiatric conditions or symptoms.

Results

In the sample of 693 men, 66% were aged between 18 and 35 years old, and 55% gave a history of TBI (“TBI+”). Overall, 55% of study participants reported at least one TBI. High levels of neuropsychiatric symptomatology were reported. In 75% of TBI+ individuals, their most severe TBI (by self-report) was associated with loss of consciousness (LOC) < 30 min. Compared to TBI- (those without history of TBI) participants, TBI+ individuals were more impulsive (Eysenck Impulsivity), irritable, angry, and reported higher levels of assaultive behavior, depressive symptomology, alcohol use disorder, suicidal ideation, suicide attempts, and lower quality of life. Potential “dose effects” of TBI severity and frequency in terms of neuropsychiatric symptomatology were identified.

Conclusion

Like other studies of offender populations, single and multiple TBIs were very common. The associations of TBI, TBI severity, and TBI frequency (i.e., TBI “burden”) with adverse neuropsychiatric phenomena suggest TBI contributes importantly to offender morbidity but the select nature of the sample and cross-sectional study design constrain the interpretation of these findings.
The effects of family environment cognition and its difference perceived by adolescents and their parents on the treatment effect of non-suicidal self-injury behaviors in adolescents: a 1-year prospective cohort study
Authors: Li Yalan , Li Xin , Li Yunge , Xiao Yu , Li Chunya , Chen Jia , Li Yao , Luo Lishi , Su Ding , Jia Juan , Cheng Haofei , Liu Tianjiao , Du Na
Published: September 2023

Introduction

Family environment is the primary environment for adolescent growth and development, which is believed to have an important impact on the occurrence of non-suicidal self-injury (NSSI) behavior in adolescents. This study aimed to explore the effects of family environment cognition and cognitive differences perceived by adolescents and their parents on the treatment effects of NSSI in adolescents and to provide more potential perspectives for NSSI treatment.

Methods

A one-year prospective longitudinal sub-cohort investigation was carried out among 199 adolescents engaged in NSSI and one of their important guardians from the Longitudinal Psychosomatic Disease Study (LoPDS). The NSSI behaviors of adolescents were evaluated at 3 months, 6 months and 1 year after enrollment. The family environment scale (FES) and NSSI Behavior Questionnaire were used as assessment tools for family environment and adolescents NSSI behaviors. Multiple linear regression was used to investigate the role of family environment perception difference in the treatment effect of adolescent NSSI.

Results

After one year of follow-up, the perceived self-injury impulse score in recent 2 weeks, self-injury impulse frequency in recent 2 weeks, total number of self-injury in recent 2 weeks decreased significantly. The higher the adolescent family cohesion (Beta: 1.130, 95% CI: 0.886,1.373; p=0.032), parental family expressiveness (Beta: 0.818, 95% CI: 0.375,1.260; p=0.037) and parental family active-recreational orientation score (Beta: 0.609, 95% CI: 0.236,0.981; p=0.048), the better the treatment effect. However, higher adolescent family conflict (Beta: -0.838, 95% CI: -1.377,-0.298; p=0.024) were associated with lower treatment outcomes. The greater the cognitive difference between parents and adolescents in family cohesion (Beta: -1.307, 95% CI: -2.074,-0.539; p=0.014) and family conflict(Beta: -0.665, 95% CI: -0.919,-0.410; p=0.037), the worse the therapeutic effect of NSSI might be.

Discussion

There were certain differences in the cognition of family relationships between parents and adolescents, and subjective family relationship cognition and cognitive differences had a significant effect on the treatment effect of NSSI in adolescents. Helping them identify the cause of cognitive differences and conducting systematic family therapy from the points of difference may be another perspective to improve the treatment effect of NSSI in adolescents.