bluuebunny/crossref_metadata_2025 huggingface dataset:
Letrozole cotreatment improves the follicular output rate in high-body-mass-index women with polycystic ovary syndrome undergoing IVF treatment
Authors: Liu Yali , Lin Jiaying , Shen Xi , Zhu Qianqian , Kuang Yanping
Published: March 2023

Background

Women who have polycystic ovary syndrome (PCOS) with high body mass index (BMI) typically have an attenuated ovarian response and decreased follicular size, which are linked to unfavourable clinical outcomes following in vitro fertilization (IVF) therapy. The follicular output rate (FORT), a qualitative indicator of follicular response, seems to be positively linked to the clinical outcomes of IVF. Progestin-primed ovarian stimulation (PPOS) has become an alternative to gonadotropin-releasing hormone (GnRH) analogues to inhibit the premature luteinizing hormone (LH) surge. As letrozole (LE) shows promise in enhancing ovarian response, we compared PPOS with and without LE for PCOS in high BMI women with a focus on the FORT and associated clinical and pregnancy outcomes.

Methods

For the recruited 1508 women, ten variables including AFC; age; basal sex hormone level; BMI; infertility type; period of infertility and number of previous IVF attempts were chosen in the propensity score matching (PSM) model to match 1374 women who taken the MPA+ hMG protocol with 134 women who received the MPA+ hMG+ LE treatment at a 1:1 ratio. FORT was selected as the primary outcome measure. The number of oocytes retrieved, viable embryos, hMG dosage, duration, oocyte maturity rate, fertilization rate, and implantation rate were established as secondary outcomes.

Results

FORT was substantially elevated in the MPA+hMG+LE group compared with the MPA+hMG group (61% [35%, 86%] vs. 40% [25%, 60%], P <.001). Interestingly, the LE cotreatment group had a considerably lower mature oocyte rate despite having a similar number of mature oocytes and embryos recovered. The average hMG dosages and durations in the study group were similar to those in the control group. The implantation rate in the study group was numerically higher but without statistic significant than that in the control groups (43.15% (107/248) vs. 38.59% (115/298), OR 1.008, 95% CI 0.901-1.127; P >.05).

Conclusion

The effect of LE combined with PPOS on FORT is better than the effect of the standard PPOS treatment in women with PCOS and a high BMI, but there is no substantially beneficial impact on pregnancy outcomes or the cycle features of COS, including consumption of hMG.
Relationship between risk factors for impaired bone health and HR-pQCT in young adults with type 1 diabetes
Authors: Sochett Etienne B. , Dominicis Mary , Vali Reza , Shammas Amer , Elia Yesmino , Moineddin Rahim , Mahmud Farid , Assor Esther , Furman Michelle , Boyd Steve K. , Lenherr-Taube Nina
Published: March 2023

Objective

In type 1 diabetes, risk factors associated with impaired bone health contribute to increased risk of fracture. The aim of this study was to (1): compare the high-resolution peripheral quantitative computed tomography (HR-pQCT) parameters of young adults with type 1 diabetes with those of healthy controls (2), identify sex differences, and (3) evaluate the association between diabetes and bone health risk factors, with HR-pQCT.

Methods

This is a cross-sectional study in young Canadian adults with childhood onset type 1 diabetes. Z-scores were generated for HR-pQCT parameters using a large healthy control database. Diet, physical activity, BMI, hemoglobin A1C (A1C) and bone health measures were evaluated, and associations were analyzed using multivariate regression analysis.

Results

Eighty-eight participants (age 21 ± 2.2 years; 40 males, 48 females, diabetes duration 13.9 ± 3.4 years) with type 1 diabetes were studied. Low trabecular thickness and elevated cortical geometry parameters were found suggesting impaired bone quality. There were no sex differences. Significant associations were found: Vitamin D (25(OH)D) with trabecular parameters with possible synergy with A1C, parathyroid hormone with cortical parameters, BMI with cortical bone and failure load, and diabetes duration with trabecular area.

Conclusions

Our data suggests impairment of bone health as assessed by HR-pQCT in young adults with type 1 diabetes. Modifiable risk factors were associated with trabecular and cortical parameters. These findings imply that correction of vitamin D deficiency, prevention and treatment of secondary hyperparathyroidism, and optimization of metabolic control may reduce incident fractures.
Chorea: An unusual manifestation of endocrine diseases
Authors: Zheng Jia , Wu Xiaohong
Published: March 2023

Chorea is a movement disorder involving involuntary movements of muscles of the face, neck, and limbs, usually caused by basal ganglia lesions. As an important part of the presentation of many neurological diseases, chorea is also an unusual manifestation of endocrine diseases and can be challenging to diagnose. Although the most common etiology of chorea is genetic, it is vital to identify acquired or symptomatic chorea, as these are potentially treatable conditions. This review summarizes the latest developments in various endocrine disease-related chorea, which will help clinicians to correctly identify and accurately treat it.
GIS integrated RUSLE model-based soil loss estimation and watershed prioritization for land and water conservation aspects
Authors: Singh Mahesh Chand , Sur Koyel , Al-Ansari Nadhir , Arya Prashant Kumar , Verma Vipan Kumar , Malik Anurag
Published: March 2023

Land degradation has become one of the major threats throughout the globe, affecting about 2.6 billion people in more than 100 countries. The highest rate of land degradation is in Asia, followed by Africa and Europe. Climate change coupled with anthropogenic activities have accelerated the rate of land degradation in developing nations. In India, land degradation has affected about 105.48 million hectares. Thus, modeling and mapping soil loss, and assessing the vulnerability threat of the active erosional processes in a region are the major challenges from the land and water conservation aspects. The present study attempted rigorous modeling to estimate soil loss from the Banas Basin of Rajasthan state, India, using GIS-integrated Revised Universal Soil Loss Equation (RUSLE) equation. Priority ranking was computed for different watersheds in terms of the degree of soil loss from their catchments, so that appropriate conservation measures can be implemented. The total area of Banas basin (68,207.82 km2) was systematically separated into 25 watersheds ranging in area from 113.0 to 7626.8 km2. Rainfall dataset of Indian Meteorological Department for 30 years (1990–2020), FAO based Soil map for soil characterization, ALOS PALSAR digital elevation model for topographic assessment, and Sentinal-2 based land use and land cover map were integrated for modeling and mapping soil erosion/loss risk assessment. The total annual soil loss in the Banas basin was recorded as 21,766,048.8 tons. The areas under very low (0–1 t ha-1 year-1), low (1–5 t ha-1 year-1), medium (5–10 t ha-1 year-1), high (10–50 t ha-1 year-1) and extreme (>50 t ha-1 year-1) soil loss categories were recorded as 24.2, 66.8, 7.3, 0.9, and 0.7%, respectively, whereas the respective average annual soil loss values were obtained as 0.8, 3.0, 6.0, 23.1, and 52.0 t ha-1 year-1. The average annual soil loss among different watersheds was recorded in the range of 1.1–84.9 t ha-1 year-1, being highest (84.9 t ha-1 year-1) in WS18, followed by WS10 (38.4 t ha-1 year-1), SW25 (34.7 t ha-1 year-1) and WS23 (17.9 t ha-1 year-1), whereas it was lowest for WS8 (1.1 t ha-1 year-1). Thus, WS18 obtained the highest/top priority rank in terms of the average annual soil loss (84.9 t ha-1 year-1) to be considered as the first priority for land and water conservation planning and implementation. The quantitative results of this study would be useful for implementation of land and water conservation measures in the problematic areas of the Banas basin for controlling soil loss through water erosion.
Diminishing influence of negative relationship between species richness and evenness on the modeling of grassland α-diversity metrics
Authors: Yan Hui , Li Fei , Liu Guixiang
Published: March 2023

Species richness and evenness have been widely used to investigate the spatiotemporal variation of α-diversity. However, some studies have indicated that a negative relationship exists between species richness and evenness. The question is how the differing sensitivity of α-diversity metrics and interactive behavior between richness and evenness affect the modeling of α-diversity variation. Here, we explored the response of species diversity, represented by three Hill numbers (i.e., species richness, exponential of Shannon index – expShannon, and inverse of Simpson index – invSimpson) focusing on the abundance of rare and common species, and Pielou index underlining the evenness of a community, to α-diversity variation through structural equation modeling (SEM). The model scheme integrated three categories of variables, spectral variation hypothesis (SVH), community pattern, and vertical structure, along the precipitation gradient spanning three steppes, including meadow steppe, typical steppe, and desert steppe. Our results showed that there were large differences in species richness across the three steppes, with v-shaped patterns emerging along the gradient (low-point in the typical steppe). Differences between steppes were diminished in the expShannon or invSimpson indices, though the v-shaped patterns persisted. The Pielou index showed the opposite pattern, with the peak in the typical steppe. Accordingly, a negative relationship between species richness and Pielou index was found across the three steppes. The concurrent increases in annual species number and dominant species abundance in response to precipitation variations led to the negative relationship. As a result, the SEM fitness on expShannon and invSimpson indices over the region was substantially diminished by the negative relationship. Overall, community pattern better explained the variation in species richness, invSimpson and Pielou indices. The performance of SVH differed among α-diversity metrics due to the collinearity with the variables of community pattern and vertical structure. This study emphasizes the variability of α-diversity metrics in response to environmental change. Particularly, distinguishing the asynchronous behaviors between species richness and evenness is paramount to account for α-diversity variation over heterogeneous ecosystems.
Both species richness and growth forms affect nutrient removal in constructed wetlands: A mesocosm experiment
Authors: Luo Yumei , Chen Quan , Liu Fan , Dai Can
Published: March 2023

Introduction

Plant richness is thought to improve the function of constructed wetlands (CWs), but most CWs are planted with monocultures, with only a few employed polycultures, which have drawn contradictory conclusions. We suppose functional diversity is the key to better performance of plant communities and hypothesize that CWs planted with diverse growth forms are superior in plant growth and nutrient removal.

Methods

In this study, six emergent plant species categorized into slender type (Schoenoplectus tabernaemontani, Typha orientalis), fan type (Iris sibirica, Acorus calamus) and large type (Canna indica and Thalia dealbata) were planted in monocultures, combinations (two species of the same growth form) and mixed polycultures (six species of three growth forms). We then compared how plant growth and nutrient uptake differed among treatments.

Results

It showed that the polyculture considerably increased the removal of total nitrogen (TN) and total phosphorus (TP), but the combination did not outperform monoculture. High consistency in the patterns between underground biomass and total biomass indicated that plant roots were essential for nutrient consumption. Compared with slender and fan plants, the large plants had a greater biomass increase in polycultures, which greatly accelerated the absorption and assimilation of TN and TP.

Conclusion

Our study indicated that plant community with various growth forms reduced the intensity of interspecific competition, increased the functional diversity, and greatly enhanced the ability of pollutant removal. Our results also provide some suggestions for plant selection and combination designs in CWs.
Environmental factors shaping copepod distributions in cave waters of the Lessinian unsaturated karst (NE-Italy)
Authors: Vaccarelli Ilaria , Cerasoli Francesco , Mammola Stefano , Fiasca Barbara , Di Cicco Mattia , Di Lorenzo Tiziana , Stoch Fabio , Galassi Diana Maria Paola
Published: March 2023

The unsaturated karst represents a transitional zone connecting the land surface to the water table of the underlying saturated zone through percolating water and small perched aquifers. It constitutes a significant groundwater reservoir and hosts a high biodiversity of specialized and endemic species. Whereas investigating this ecotone can shed light on the invertebrate diversity of the whole karst system, ecological studies in the unsaturated karst have been hampered by access, mapping, and sampling challenges. We studied the unsaturated zone of 52 caves in the Lessinian Massif (NE-Italy), which is dominated by carbonate rocks interspersed with spot areas composed mainly of igneous rocks. Our aim was to understand which ecological and historical factors may have shaped the distribution of the groundwater crustacean copepods at a regional spatial scale in the Lessinian unsaturated karst. We collected 33 species belonging to the orders Harpacticoida and Cyclopoida. Species richness estimators and related species accumulation curves suggest an exhaustive sampling effort. A hydrologic analysis performed in a Geographic Information System environment divided the study area in three distinct drainage basins: Eastern Lessini, Lessinian Core, and Western Lessini. Regression analyses indicated that richness of non-stygobitic copepods (i.e., surface-water species which exploit groundwater for different purposes) was mainly driven by inter-basin differences. Richness of stygobitic copepods (i.e., obligate groundwater dwellers) was instead strongly related to lithological features and responded negatively to surface thermal variability. Next, we used a kernel density estimation to spatially explore diversity patterns in the area. The highest density of stygobitic harpacticoids was identified in the Eastern Lessinian basin, which is lithologically more heterogeneous and surrounded by practically non-aquiferous igneous rocks that may limit dispersal. Conversely, the Western Lessinian basin hosted a high density of stygobitic cyclopoid species. This spatial pattern suggests different colonization pathways for the two taxa, with a strong effect of igneous rocks in hampering dispersal of most harpacticoids. Lithological barriers seemingly did not affect non-stygobitic copepods, which were found across the whole area. Altogether, these results emphasize the key role played by the interaction of hydrological features, climate, and lithology in determining different copepod biodiversity patterns in the unsaturated karst.
Development of alternative herbals remedy for gastric cancer based on transcriptomic analysis of immune infiltration and ferroptosis
Authors: Li Mingyue , Tao Jie , Qian Rui , Jiang Feng , Song Yinzhi , Zeng Zhicong , Cai Changlong
Published: March 2023

Objective: Screening out potential herbal medicines and herbal ingredients for the treatment of gastric cancer based on transcriptomic analysis of immune infiltration and ferroptosis.

Methods: Gene expression profiles of gastric tumour tissues and normal tissue samples were obtained from the GEO database and the samples were analysed for immune cell infiltration condition and differential expressed genes of ferroptosis. Key genes were screened by protein-protein interaction (PPI) and enrichment analysis, and molecular docking was used to predict and preliminary validate potential herbal and traditional Chinese medicine components for gastric cancer based on the key genes. Finally, RT-QPCR was used to validate the prediction results.

Results: Immune cell infiltration analysis revealed high levels of infiltration of activated CD4 memory T cells, monocytes, M0 macrophages in gastric tumor tissues, while plasma cells and resting mast cells had higher levels of infiltration in the paraneoplastic tissues. Differential gene expression analysis identified 1,012 upregulated genes and 880 downregulated genes, of which 84 immune related differentially expressed genes such as CTSB, PGF and PLAU and 10 ferroptosis-related differentially expressed genes such as HSF1, NOX4 and NF2 were highly expressed in gastric cancer tissues. The results of enrichment analysis showed that they mainly involve 343 biological processes such as extracellular matrix organization and extracellular structural organization; 37 cellular components such as complexes of collagen trimer and basement membrane; 35 molecular functions such as signal receptor activator activity and receptor ligand activity; 19 regulatory pathways such as cytokine-cytokine receptor interactions and retinol metabolism. Finally, two key genes, TLR4 and KRAS, were selected and 12 herbal medicines such as Radix Salviae liguliobae, Rhizoma Coptidis, Rhizoma Polygoni cuspidati and 27 herbal ingredients such as resveratrol, salvianolic acid b were predicted on the basis of key genes. Molecular docking results showed that KRAS binds tightly to coumarin and magnolol, while TLR4 can bind tightly to resveratrol, curcumin, salvianolic acid b, shikonin. Subsequently, the effect of resveratrol and magnolol was experimentally verified.

Conclusion: Herbal medicines such as S. liguliobae, Rhizoma Coptidis, Rhizoma P. cuspidati and herbal ingredients such as resveratrol, curcumin, salvianolic acid b may provide research directions and alternative therapeutic approaches for immunomodulation of TME and ferroptosis of tumour cells in gastric cancer.
Increased IKKϵ protein stability ensures efficient type I interferon responses in conditions of TBK1 deficiency
Authors: Wegner Julia , Hunkler Charlotte , Ciupka Katrin , Hartmann Gunther , Schlee Martin
Published: March 2023

TBK1 and IKKϵ are related, crucial kinases in antiviral immune signaling pathways downstream of cytosolic nucleic acid receptors such as cGAS and RIG-I-like receptors. Upon activation, they phosphorylate the transcription factors IRF3 and IRF7 and thereby initiate the expression of type I interferons and antiviral effectors. While point mutation-induced loss of TBK1 kinase activity results in clinical hyper-susceptibility to viral infections, a complete lack of TBK1 expression in humans is unexpectedly not associated with diminished antiviral responses. Here, we provide a mechanistic explanation for these so-far unexplained observations by showing that TBK1 controls the protein expression of its related kinase IKKϵ in human myeloid cells. Mechanistically, TBK1 constitutively diminishes the protein stability of IKKϵ independent of TBK1 kinase activity but dependent on its interaction with the scaffold protein TANK. In consequence, depletion of TBK1 protein but not mutation-induced kinase deficiency induces the upregulation of IKKϵ. Due to the functional redundancy of the kinases in cGAS-STING and RIG-I-like receptor signaling in human myeloid cells, enhanced IKKϵ expression can compensate for the loss of TBK1. We show that IKKϵ upregulation is crucial to ensure unmitigated type I interferon production in conditions of TBK1 deficiency: While the type I interferon response to Listeria monocytogenes infection is maintained upon TBK1 loss, it is strongly diminished in cells harboring a kinase-deficient TBK1 variant, in which IKKϵ is not upregulated. Many pathogens induce TBK1 degradation, suggesting that loss of TBK1-mediated destabilization of IKKϵ is a critical backup mechanism to prevent diminished interferon responses upon TBK1 depletion.
Complement biomarkers reflect the pathological status of neuromyelitis optica spectrum disorders
Authors: Miyamoto Katsuichi , Minamino Mai , Kuwahara Motoi , Tsujimoto Hiroshi , Ohtani Katsuki , Wakamiya Nobutaka , Katayama Kei-ichi , Inoue Norimitsu , Ito Hidefumi
Published: March 2023

Complement is involved in the pathogenesis of neuroimmune disease, but the detailed pathological roles of the complement pathway remain incompletely understood. Recently, eculizumab, a humanized anti-C5 monoclonal antibody, has been clinically applied against neuroimmune diseases such as myasthenia gravis and neuromyelitis optica spectrum disorders (NMOSD). Clinical application of eculizumab is also being investigated for another neuroimmune disease, Guillain-Barré syndrome (GBS). However, while the effectiveness of eculizumab for NMOSD is extremely high in many cases, there are some cases of myasthenia gravis and GBS in which eculizumab has little or no efficacy. Development of effective biomarkers that reflect complement activation in these diseases is therefore important. To identify biomarkers that could predict disease status, we retrospectively analyzed serum levels of complement factors in 21 patients with NMOSD and 25 patients with GBS. Ba, an activation marker of the alternative complement pathway, was elevated in the acute phases of both NMOSD and GBS. Meanwhile, sC5b-9, an activation marker generated by the terminal complement pathway, was elevated in NMOSD but not in GBS. Complement factor H (CFH), a complement regulatory factor, was decreased in the acute phase as well as in the remission phase of NMOSD, but not in any phases of GBS. Together, these findings suggest that complement biomarkers, such as Ba, sC5b-9 and CFH in peripheral blood, have potential utility in understanding the pathological status of NMOSD.
Neutrophils as a potential therapeutic target in Alzheimer’s disease
Authors: Aries Michelle L. , Hensley-McBain Tiffany
Published: March 2023

Alzheimer’s disease (AD) is the leading cause of dementia in the United States. Sporadic or late-onset AD remains incompletely understood, with age as the current greatest risk factor. Inflammation in general and neutrophils, a potent mediator of inflammation, have been shown to exacerbate AD associated dementia. This review explores the latest research on neutrophils in AD mouse models and in human cohort studies and discusses current gaps in research and needs for future studies. AD mouse models have shown neutrophil chemotactic migration towards amyloid beta plaques in the brain. Capillary blood flow stalling decreases blood perfusion to associated brain regions and mouse studies have demonstrated that anti-Ly6G antibodies lead to a decrease in capillary blood flow stalling and memory improvement. Several recent transcriptomic studies of blood and brain tissue from persons with AD have shown an upregulation in neutrophil-related genes, and studies have demonstrated neutrophil involvement in brain capillary adhesion, blood brain barrier breaching, myeloperoxidase release, and the propensity for neutrophil extracellular trap release in AD. Neutrophil-derived inflammation and regulation are a potential potent novel therapeutic target for AD progression. Future studies should further investigate neutrophil functionality in AD. In addition, other aspects of AD that may impact neutrophils including the microbiome and the APOE4 allele should be studied.
Efficacy and safety of Iguratimod in the treatment of Ankylosing Spondylitis: A systematic review and meta-analysis of randomized controlled trials
Authors: Long Zhiyong , Deng Ying , He Qi , Yang Kailin , Zeng Liuting , Hao Wensa , Deng Yuxuan , Fan Jiapeng , Chen Hua
Published: March 2023

Objective

To explore the efficacy and safety of Iguratimod (IGU) intervention in the treatment of Ankylosing Spondylitis (AS).

Methods

We used computer to search literature databases, collected randomized controlled trials (RCTs) related to IGU treatment of AS, and searched the relevant literature in each database until Sep. 2022. Two researchers independently carried out literature screening, data extraction, and evaluation and analysis of the risk of bias in the included studies, and then used Rev Man5.3 software for meta-analysis. The protocol is CRD42020220798.

Results

A total of 10 RCTs involves in 622 patients were collected. The statistical analysis showed that IGU can decrease the BASDAI score (SMD -1.62 [-2.20, -1.05], P<0.00001. Quality of evidence: low), the BASFI score (WMD -1.30 [-1.48, -1.12], P<0.00001. Quality of evidence: low) and the VAS (WMD -2.01 [-2.83, -1.19], P<0.00001. Quality of evidence: very low). Meanwhile, the addition of IGU into the conventional therapy would not increase the adverse events (RR 0.65 [0.43, 0.98], P=0.04. Quality of evidence: moderate).

Conclusion

IGU may be an effective and safe intervention for AS.

Systematic review registration

https://www.crd.york.ac.uk/prospero/display_record.php?, identifier CRD42020220798.
Genome-wide DNA methylation and transcription analysis reveal the potential epigenetic mechanism of heat stress response in the sea cucumber Apostichopus japonicus
Authors: Chang Mengyang , Ge Jianlong , Liao Meijie , Rong Xiaojun , Wang Yingeng , Li Bin , Li Xinrong , Wang Jinjin , Zhang Zheng , Yu Yongxiang , Wang Chunyuan
Published: March 2023

DNA methylation is an important epigenetic modification that regulates many biological processes. The sea cucumber Apostichopus japonicus often suffers from heat stress that affects its growth and leads to significant economic losses. In this study, the mRNA expression patterns and DNA methylation characteristics in the body wall of A. japonicus under heat stress were analyzed by whole-genome bisulfite sequencing (WGBS) and transcriptome sequencing (RNA-seq). We found that CpG was the main DNA methylation type, and heat stress caused a significant increase in the overall methylation level and methylation rate, especially in the intergenic region of the A. japonicus genome. In total, 1,409 differentially expressed genes (DEGs) and 17,927 differentially methylated genes (DMGs) were obtained by RNA-seq and WGBS, respectively. Association analysis between DNA methylation and transcription identified 569 negatively correlated genes in both DMGs and DEGs, which indicated that DNA methylation affects on transcriptional regulation in response to heat stress. These negatively correlated genes were significantly enriched in pathways related to energy metabolism and immunoregulation, such as the thyroid hormone signaling pathway, renin secretion, notch signaling pathway and microRNAs in cancer. In addition, potential key genes, including heat shock protein (hsp70), calcium-activated chloride channel regulator 1(clca1), and tenascin R (tnr), were obtained and their expression and methylation were preliminarily verified. The results provide a new perspective for epigenetic and transcriptomic studies of A. japonicus response to heat stress, and provide a reference for breeding sea cucumbers resistant to high temperatures.
Analysis of the electrically conducting magnetohydrodynamic hybrid nanofluid flow past a convectively heated stretching surface with suction/injection and non-linear thermal radiation
Authors: Algehyne Ebrahem A. , Lone Showkat Ahmad , Raizah Zehba , Eldin Sayed M. , Saeed Anwar , Galal Ahmed M.
Published: March 2023

Fluid flow through a porous media has many industrial applications such as water flowing through rocks and soil and purification of gas and oil mixed in rocks. Also, heat transfer enhancement has been introduced in various thermal and mechanical systems by improving the thermal conductance of base fluids. In this article, the flow of an electrically conducting water-based hybrid nanofluid comprising GO and Fe3O4 nanoparticles over an extending sheet using a porous medium has been investigated. The space-dependent heat source, Joule heating, Brownian motion, thermophoresis, thermal radiation, chemical reaction, and activation energy impacts are taken into account. For the solution of the modeled equations, the homotopy analysis method is considered. The homotopic convergence is shown with the help of a figure. This analysis is contrasted with previous outcomes and has found a great agreement. The impacts of embedded factors on different flow characteristics, skin friction coefficient, and Nusselt and Sherwood numbers are displayed using figures and tables. The outcomes of the present analysis show that the increasing magnetic and suction factors have reduced the fluid motion while amplifying the thermal profiles. Additionally, the suction factor has a reducing impact on both temperature and concentration profiles. The thermal profiles have increased with the increasing thermal Biot number, Eckert number, thermophoresis, and Brownian motion factors. The Nusselt numbers have increased with the increasing thermal Biot number and stretching factor but reduced with the increasing thermal radiation and temperature difference factors.
Durability behavior of asphalt mixtures in regard to material properties and gradation type
Authors: Ji Tonggeng , Hao Peiwen , She Hongwei , Yang Kai , Li Hongxiang , Wang Dong , Kang Rui , Liu Jingwen
Published: March 2023

The main objective of this study is to compare the differences between different gradation types of mixtures (stone matrix asphalt (SMA), large stone asphalt (LSA), stone asphalt concrete (SAC), and superior performing asphalt pavements (Superpave)) and conventional dense-graded mixtures (asphalt concrete (AC)) in terms of resistance to rutting, moisture damage, low-temperature cracking, and water permeability. The paper reports a study of the effect of different asphalt binders on the performance of asphalt mixtures. The experimental results demonstrated that the gradation types had a significant effect on the engineering performance of asphalt mixtures. SMA20, SAC20, and LSA30 exhibited lower rutting potential than AC20. In addition, SAC20 had a larger stripping ratio, while AC13 had a smaller stripping ratio. The stone matrix asphalt displayed the largest failure strain, while the SAC had the smallest, and Superpave ranged between SMA and SAC. SAC20 had the maximum permeability coefficient, followed by SMA20 and AC20, and Superpave20 had the smallest. The nominal maximum aggregate sizes significantly affected the resistance to permanent deformation, resistance to low-temperature cracking, and moisture sensitivity of the asphalt mixtures. Styrene–butadiene–styrene (SBS)-modified asphalt was found to significantly improve the performance of asphalt mixtures compared with ordinary asphalt.
Assessment of a novel continuous cleaning device using metatranscriptomics in diverse hospital environments
Authors: Wright Justin R , Ly Truc T , Cromwell Karen B , Brislawn Colin J , Chen See Jeremy R , Anderson Samantha LC , Pellegrino Jordan , Peachey Logan , Walls Christine Y , Lloyd Charise M , Jones Olcay Y , Lawrence Matthew W , Bess Jessica A , Wall Arthur C , Shope Alexander J , Lamendella Regina
Published: March 2023

Introduction

Despite routine implementation of cleaning and disinfection practices in clinical healthcare settings, high-touch environmental surfaces and contaminated equipment often serve as reservoirs for the transmission of pathogens associated with healthcare-associated infections (HAIs).

Methods

The current study involved the analysis of high-touch surface swabs using a metatranscriptomic sequencing workflow (CSI-Dx™) to assess the efficacy of cleanSURFACES® technology in decreasing microbial burden by limiting re-contamination. This is a non-human single center study conducted in the Emergency Department (ED) and on an inpatient Oncology Ward of Walter Reed National Military Medical Center that have followed hygienic practices during the COVID-19 pandemic environment.

Results

Although there was no difference in observed microbial richness (two-tailed Wilcoxon test with Holm correction, P > 0.05), beta diversity findings identified shifts in microbial community structure between surfaces from baseline and post-intervention timepoints (Day 1, Day 7, Day 14, and Day 28). Biomarker and regression analyses identified significant reductions in annotated transcripts for various clinically relevant microorganisms' post-intervention, coagulase-negative staphylococci and Malassezia restricta, at ED and Oncology ward, respectively. Additionally, post-intervention samples predominantly consisted of Proteobacteria and to a lesser extent skin commensals and endogenous environmental microorganisms in both departments.

Discussion

Findings support the value of cleanSURFACES®, when coupled with routine disinfection practices, to effectively impact on the composition of active microbial communities found on high-touch surfaces in two different patient care areas of the hospital (one outpatient and one inpatient) with unique demands and patient-centered practices.
Nanotechnology in combating biofilm: A smart and promising therapeutic strategy
Authors: Mohanta Yugal Kishore , Chakrabartty Ishani , Mishra Awdhesh Kumar , Chopra Hitesh , Mahanta Saurov , Avula Satya Kumar , Patowary Kaustuvmani , Ahmed Ramzan , Mishra Bibhudutta , Mohanta Tapan Kumar , Saravanan Muthupandian , Sharma Nanaocha
Published: March 2023

Since the birth of civilization, people have recognized that infectious microbes cause serious and often fatal diseases in humans. One of the most dangerous characteristics of microorganisms is their propensity to form biofilms. It is linked to the development of long-lasting infections and more severe illness. An obstacle to eliminating such intricate structures is their resistance to the drugs now utilized in clinical practice (biofilms). Finding new compounds with anti-biofilm effect is, thus, essential. Infections caused by bacterial biofilms are something that nanotechnology has lately shown promise in treating. More and more studies are being conducted to determine whether nanoparticles (NPs) are useful in the fight against bacterial infections. While there have been a small number of clinical trials, there have been several in vitro outcomes examining the effects of antimicrobial NPs. Nanotechnology provides secure delivery platforms for targeted treatments to combat the wide range of microbial infections caused by biofilms. The increase in pharmaceuticals’ bioactive potential is one of the many ways in which nanotechnology has been applied to drug delivery. The current research details the utilization of several nanoparticles in the targeted medication delivery strategy for managing microbial biofilms, including metal and metal oxide nanoparticles, liposomes, micro-, and nanoemulsions, solid lipid nanoparticles, and polymeric nanoparticles. Our understanding of how these nanosystems aid in the fight against biofilms has been expanded through their use.
Evaluation of stirring time through a rumen simulation technique: Influences on rumen fermentation and bacterial community
Authors: Wang Zuo , Li Quan , Lan Xinyi , Shen Weijun , Wan Fachun , He Jianhua , Tang Shaoxun , Tan Zhiliang
Published: March 2023

Introduction

Rumen motility is a key element that influences ruminant nutrition, whereas little is known about the effects of rumen contraction duration on rumen fermentation and ruminal microbiome. We previously reported that proper rotation speed of a rumen simulation technique (RUSITEC) system enhanced rumen fermentation and microbial protein (MCP) production. In the present study, different contraction durations and intervals were simulated by setting different stirring times and intervals of the stirrers in a RUSITEC system. The objective of this trial was to evaluate the influences of stirring time on rumen fermentation characteristics, nutrient degradation, and ruminal bacterial microbiota in vitro.

Methods

This experiment was performed in a 3 × 3 Latin square design, with each experimental period comprising 4 d for adjustment and 3 d for sample collection. Three stirring time treatments were set: the constant stir (CS), the intermittent stir 1 (each stir for 5 min with an interval of 2 min, IS1), and the intermittent stir 2 (each stir for 4 min with an interval of 3 min, IS2).

Results

The total volatile fatty acid (TVFA) concentration, valerate molar proportion, ammonia nitrogen level, MCP density, protozoa count, disappearance rates of dry matter, organic matter, crude protein, neutral detergent fiber, and acid detergent fiber, emissions of total gas and methane, and the richness index Chao 1 for the bacterial community were higher (p < 0.05) in the IS1 when compared to those in the CS. The greatest TVFA, MCP, protozoa count, nutrient disappearance rates, gas productions, and bacterial richness indices of Ace and Chao 1 amongst all treatments were observed in the IS2. The relative abundance of the genus Treponema was enriched (p < 0.05) in CS, while the enrichment (p < 0.05) of Agathobacter ruminis and another two less known bacterial genera were identified in IS2.

Discussion

It could be concluded that the proper reduction in the stirring time might help to enhance the feed fermentation, MCP synthesis, gas production, and the relative abundances of specific bacterial taxa.
HPLC-MS-MS quantification of short-chain fatty acids actively secreted by probiotic strains
Authors: Calvigioni Marco , Bertolini Andrea , Codini Simone , Mazzantini Diletta , Panattoni Adelaide , Massimino Mariacristina , Celandroni Francesco , Zucchi Riccardo , Saba Alessandro , Ghelardi Emilia
Published: March 2023

Introduction

Short-chain fatty acids (SCFAs) are the main by-products of microbial fermentations occurring in the human intestine and are directly involved in the host’s physiological balance. As impaired gut concentrations of acetic, propionic, and butyric acids are often associated with systemic disorders, the administration of SCFA-producing microorganisms has been suggested as attractive approach to solve symptoms related to SCFA deficiency.

Methods

In this research, nine probiotic strains (Bacillus clausii NR, OC, SIN, and T, Bacillus coagulans ATCC 7050, Bifidobacterium breve DSM 16604, Limosilactobacillus reuteri DSM 17938, Lacticaseibacillus rhamnosus ATCC 53103, and Saccharomyces boulardii CNCM I-745) commonly included in commercial formulations were tested for their ability to secrete SCFAs by using an improved protocol in high-performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS-MS).

Results

The developed method was highly sensitive and specific, showing excellent limits of detection and quantification of secreted SCFAs. All tested microorganisms were shown to secrete acetic acid, with only B. clausii and S. boulardii additionally able to produce propionic and butyric acids. Quantitative differences in the secretion of SCFAs were also evidenced.

Discussion

The experimental approach described in this study may contribute to the characterization of probiotics as SCFA-producing organisms, a crucial stage toward their application to improve SCFA deficiency.
Reliability of the modified Rankin Scale in clinical practice of stroke units and rehabilitation wards
Authors: Pożarowszczyk Natalia , Kurkowska-Jastrzębska Iwona , Sarzyńska-Długosz Iwona , Nowak Maciej , Karliński Michał
Published: March 2023

Introduction

The Modified Rankin Scale (mRS) is the most common tool to quantify post-stroke disability in everyday practice and by certified raters in clinical trials. However, interobserver variability may affect reliability of retrospective observational studies, including clinical registries. Our aim was to assess real-life consistency between neurologists and physical and rehabilitation medicine physicians using mRS to rate post-stroke disability of patients transferred directly from stroke unit (SU) to rehabilitation ward (RW).

Methods

This is a retrospective analysis of 132 consecutive acute stroke patients transferred from single tertiary SU to RW located in the same hospital in Poland. Patients were assessed by one rater from each department at the day of transfer. We distinguished between physicians previously certified in using mRS for clinical trials and not-certified physicians using mRS in everyday practice.

Results

mRS at discharge from SU and on admission to RW was recorded for 105 of 132 patients. The overall agreement was 70.5% (kappa 0.55). Similar agreement was observed in the subset of 30 patients rated by certified physicians in both departments (70.0%, kappa 0.57) and in the subset of 61 patients rated by a pair of certified neurologist and not-certified rehabilitation physician (73.8%, kappa 0.58).

Conclusions

Everyday consistency between raters from SU and RW in using mRS is modest as in previous validation studies. However, it may be considered sufficient for the purpose of observational studies or stroke registries. It emphasizes the need for easily accessible training in conventional mRS or implementation of specialized tools with predefined questions.
Serum metabolites as early detection markers of non-muscle invasive bladder cancer in Chinese patients
Authors: Zhao Yi , Sun Wei , Ji Zhigang , Liu Xiaoyan , Qiao Yi
Published: March 2023

Background

Biomarkers of different stages and grades of bladder cancer (BC) are important in clinical work. The objective of our study was to investigate new biomarkers of early-stage BC with liquid chromatography-high resolution mass spectrometry (LC-HRMS) using serum samples.

Methods

A total of 215 cases were included in our study, including 109 healthy adults as the control group and 106 non-muscle invasive bladder cancer (NMIBC) patients as the NMIBC group. Serum samples were collected from BC patients in the early stage, called NMIBC, and healthy people before surgery. We used LC-HRMS to distinguish the NMIBC group from the control group and the low-grade NMIBC group from the control group.

Results

An apparent difference between the NMIBC group and the control group was visualized by unsupervised principal component analysis (PCA). Metabolite panels for 16-hydroxy-10-oxohexadecanoic acid, PGF2a ethanolamide, sulfoglycolithocholate, and threoninyl-alanine were used to distinguish the two groups. The area under the curve (AUC) of the panels was 0.985, and the sensitivity and specificity were 98.63% and 98.59%, respectively. To distinguish the low-grade NMIBC group from the control group, serum metabolic profiling differences between the low-grade NMIBC group and control group samples were also analyzed. Metabolite panels of L-octanoylcarnitine, PGF2a ethanolamide, and threoninyl-alanine showed good discrimination performance. The AUC of the panels was 0.999, and the sensitivity and specificity were 97.8% and 100%, respectively.

Conclusion

Metabolomics analysis of serum samples can distinguish the NMIBC group from the control group, particularly the early-stage low-grade NMIBC group.
Advances in the treatment of Hodgkin lymphoma: Current and future approaches
Authors: Ullah Fauzia , Dima Danai , Omar Najiullah , Ogbue Olisaemeka , Ahmed Sairah
Published: March 2023

Hodgkin lymphoma (HL) is a rare type of lymphoma with unique histologic, immunophenotypic, and clinical features. It represents approximately one-tenth of lymphomas diagnosed in the United States and consists of two subtypes: classical Hodgkin’s lymphoma (cHL), which accounts for majority of HL cases, and nodular lymphocyte predominant Hodgkin lymphoma represent approximately 5% of Hodgkin lymphoma cases. From this point, we will be focusing on cHL in this review. In general, it is considered a highly curable disease with first-line chemotherapy with or without the addition of radiotherapy. However, there are patients with disease that relapses or fails to respond to frontline regimens and the standard treatment modality for chemo sensitive cHL is high dose chemotherapy followed by autologous hematopoietic stem cell transplant (AHSCT). In recent years, targeted immunotherapy has revolutionized the treatment of cHL while many novel agents are being explored in addition to chimeric antigen receptor (CAR) T-cell therapy which is also being investigated in clinical trials as a potential treatment option.
The inhibitory effect of 6-gingerol and cisplatin on ovarian cancer and antitumor activity: In silico, in vitro, and in vivo
Authors: Salari Zohreh , Khosravi Ahmad , Pourkhandani Elham , Molaakbari Elaheh , Salarkia Ehsan , Keyhani Alireza , Sharifi Iraj , Tavakkoli Hadi , Sohbati Samira , Dabiri Shahriar , Ren Guogang , Shafie’ei Mohammad
Published: March 2023

Background

Epithelial ovarian cancer is very common in women and causes hundreds of deaths per year worldwide. Chemotherapy drugs including cisplatin have adverse effects on patients’ health. Complementary treatments and the use of herbal medicines can help improve the performance of medicine. 6-Gingerol is the major pharmacologically active component of ginger. In this study, we compared the effects of 6-gingerol, cisplatin, and their combination in apoptotic and angiogenetic activities in silico, in test tubes, and in in vivo assays against two ovarian cancer cell lines: OVCAR-3 and human umbilical vein endothelial cells (HUVECs).

Methods

The drug-treated cell lines were evaluated for their cytotoxicity, cell cycle, and apoptotic and angiogenetic gene expression changes.

Results

The proportion of apoptosis treated by 6-gingerol coupled with cisplatin was significantly high. In the evaluation of the cell cycle, the combination therapy also showed a significant promotion of a higher extent of the S sequence. The expression of p53 level, Caspase-8, Bax, and Apaf1 genes was amplified again with combination therapy. Conversely, in both cell lines, the cumulative drug concentrations reduced the expression of VEGF, FLT1, KDR, and Bcl-2 genes. Similarly, in the control group, combination treatment significantly decreased the expression of VEGF, FLT1, KDR, and Bcl-2 genes in comparison to cisplatin alone.

Conclusions

The findings of the present study demonstrated that the cisplatin and 6-gingerol combination is more effective in inducing apoptosis and suppressing the angiogenesis of ovarian cancer cells than using each drug alone.
Self-supervised contrastive learning using CT images for PD-1/PD-L1 expression prediction in hepatocellular carcinoma
Authors: Xie Tianshu , Wei Yi , Xu Lifeng , Li Qian , Che Feng , Xu Qing , Cheng Xuan , Liu Minghui , Yang Meiyi , Wang Xiaomin , Zhang Feng , Song Bin , Liu Ming
Published: March 2023

Background and purpose

Programmed cell death protein-1 (PD-1) and programmed cell death-ligand-1 (PD-L1) expression status, determined by immunohistochemistry (IHC) of specimens, can discriminate patients with hepatocellular carcinoma (HCC) who can derive the most benefits from immune checkpoint inhibitor (ICI) therapy. A non-invasive method of measuring PD-1/PD-L1 expression is urgently needed for clinical decision support.

Materials and methods

We included a cohort of 87 patients with HCC from the West China Hospital and analyzed 3094 CT images to develop and validate our prediction model. We propose a novel deep learning-based predictor, Contrastive Learning Network (CLNet), which is trained with self-supervised contrastive learning to better extract deep representations of computed tomography (CT) images for the prediction of PD-1 and PD-L1 expression.

Results

Our results show that CLNet exhibited an AUC of 86.56% for PD-1 expression and an AUC of 83.93% for PD-L1 expression, outperforming other deep learning and machine learning models.

Conclusions

We demonstrated that a non-invasive deep learning-based model trained with self-supervised contrastive learning could accurately predict the PD-1 and PD-L1 expression status, and might assist the precision treatment of patients withHCC, in particular the use of immune checkpoint inhibitors.