bluuebunny/crossref_metadata_2025 huggingface dataset:
Characterizing the vaginal microbiome in a sexually fluid primate (Pan paniscus)
Authors: Samartino Shelby D. , Bartlett Thad Q. , Wikberg Eva C.
Published: March 2024

While there have been recent breakthroughs in human vaginal microbiome research, very few non-human primate (NHP) vaginal microbiome studies exist due to difficulty in obtaining samples. In this study, we sought to: (1) characterize the bonobo vaginal microbiota for the first time, and (2) determine the relationship between vaginal pH and swelling size. During a 21-day study period, we collected observational data and 71 vaginal swabs from three cohoused adult females at the Ape Cognition and Conservation Initiative. After filtering and classification, a total of 3452 amplicon sequence variants were recruited from 12 vaginal samples. The most dominant phylum represented was Actinobacteria, and the most abundant genera were Gardnerella, Atopobium, and Prevotella. The mean pH score was 6.1 (range: 5.1-7.0), and pH levels varied with relative swelling size. This is the first study to examine the vaginal microbial composition in this species, and it conforms to previous NHP studies in that there was not the same bacterial dominance of Lactobacillus spp. often highlighted in human vaginal microbiota studies. Our findings suggest there may be other factors contributing to the protection of the bonobo vaginal environment, but future analysis of a larger sample is needed to fully understand how sociality and sexuality shape vaginal microbiota and host health.
Bursaphelenchus zealandicus sp. n. (Tylenchina: Aphelenchoididae), a new species of the hofmanni-group from Pinus radiata in New Zealand
Authors: Zhao Zeng Qi , Gu Jianfeng , Greenwood Tanya , Rogan Brent , Ho Wellcome , Taylor Robert
Published: March 2024

Bursaphelenchus zealandicus sp. n. was extracted from a dead pine tree (Pinus radiata) in Kinleith Forest, Taupo, New Zealand (NZ). This is the fourth Bursaphelenchus species from NZ. Bursaphelenchus zealandicus sp. n. is characterised by a body length of 562 (466-730) μm and 580 (470-706) μm of males and females, respectively, slim body (a = 34.7 and 33.1, respectively), stylet 11.4 (9.7-13.0) μm long for males and 10.7 (9.4-12.1) μm for females, lateral field with three lines, the mean value of the vulva position 71.8%, lacking a vulval flap in lateral view, length of the post-uterine branch ca 41.6% of the vulva-anus distance, the female tail long and conical (c = 19.3), tapering to a finely rounded terminus, ventrally curved like a hook, the male with spicules 12.3 μm long in chord, having an obvious developed rostrum and condylus, the rostrum sharply pointed, the male tail terminus with a distinct bursa seen in dorso-ventral position, a single pre-anal papilla, two pairs of ventro-sublateral papillae (one pair pre-anal (P2), one pair post-anal (P3) just in front of the bursa) and one pair of ventral mid-line glandular papillae (P4) present. The combination of three lateral lines and the position of caudal papillae reveal a relationship of the new species to other three-lined groups (hofmanni-group, eggersi-group, eremus-group, leoni-group); however, the shape of the spicules is different and unique among the known species of Bursaphelenchus. Its taxonomic status has been confirmed by analyses of the near full length small subunit (SSU), the internal transcribed spacer region (ITS1 and 2), D2-D3 expansion segments of the large subunit (LSU) of the ribosomal rDNA and the mitochondrial cytochrome c oxidase subunit I (COI) gene.
Designing Bespoke Visual Mediation Tools Using ‘Viscourse’ for Intergenerational Research
Authors: Ruscoe Amelia , Baker Penny
Published: March 2024

Visual methods are an innovative design space for study methodologies with young children. The accessibility of visual media, and flexibility of their design and use, has spurred methodological innovations that stretch the boundaries of intergenerational research. This article explores the visual dialogic nexus in research methods tailored to investigate discourse. The research sought to uncover the perspectives of young children and their teachers about their discursive affordances in the first year of school. Employing an iterative design process, bespoke visual mediation tools were collaboratively created with a visual artist to capture the intergenerational viewpoints of the participants. This article reconceptualises discourses as ‘viscourses’ through a Foucauldian post-structuralist lens. This reframing emphasizes the impact of the discursive gaze and manipulation of art elements and principles as themes for scrutiny during the design phase. The resulting visual mediation tools underwent pilot testing with two focus groups of 5-year-old children and their class teachers. Findings from the pilot study underscore the potential of visual mediation tools for generating authentic contexts that enable participants to ‘inhabit’ a time and place within a semiotic space. The method facilitates capture of multi-faceted data, including evidence of children’s higher order thinking concerning abstract phenomenon.
Student Experiences of Implementation of Accommodations
Authors: DeKorver Brittland , Brown Gordon , Witcher Shontaye
Published: March 2024

Teachers want their students to learn, and they want that learning to be equitable, with opportunities for all students. But not all students have the same needs for success. Therefore, classroom accommodations are provided through Disability Offices within universities. These accommodations are intended to be implemented in each course the student takes. Yet the actual enactment of accommodations relies on facilitation and implementation by each instructor in collaboration with the student, which may lead to variance in the level of accommodations. This project examined student interviews and student and faculty survey data to construct a narrative analysis of students’ experiences when they discuss their accommodations with their instructors. This manuscript reports a characterization of these interactions and how these interactions shape students’ success in the classroom, with recommendations for both students and instructors.
Data Assimilation Impact of GNSS RO Measurements from Cube Satellites on Arctic Weather Forecasts
Authors: Choi Yonghan , Kim Joowan , Kim Joo-Hong , Cha Dong-Hyun
Published: March 2024

In this study, the effects of assimilating Global Navigation Satellite System (GNSS) radio occultation (RO) observations from existing and recently added commercial cube satellites on analyses and forecasts over the Arctic region were investigated by conducting observing system experiments (OSEs). Profiles of refractivity were assimilated with a local observation operator using the three-dimensional variational method. The analyses and forecasts from the OSEs were verified against ERA5 reanalysis, radiosonde observations, and buoy observations. In addition to the averaged impact on forecast skill, the impact of GNSS RO observations was further examined for an individual Arctic cyclone case, focusing on the added value of the cube satellite data. The effects of GNSS RO observations from existing satellites on analyses and forecasts over the Arctic region are positive, and the assimilation of GNSS RO observations from cube satellites leads to additional improvements, particularly for temperature in the upper troposphere and lower stratosphere (UTLS). Temperature biases in the UTLS are significantly reduced in the analyses, and the improved analyses result in better forecasts of upper-level potential vorticity and cyclone development when GNSS RO observations from cube satellites are assimilated. This result demonstrates the potential of GNSS RO data from cube satellites to enhance forecasts over the Arctic region.
The First Operational Version of Taiwan Central Weather Bureau’s One-Tier Global Atmosphere–Ocean Coupled Forecast System for Seasonal Prediction
Authors: Juang Hann-Ming Henry , Wu Tzu-Yu , Liu Pang-Yen Brian , Lin Hsin-Yi , Lee Ching-Teng , Kueh Mien-Tze , Fan Jia-Fong , Chen Jen-Her River , Lu Mong-Ming , Lin Pay-Liam
Published: March 2024

The first version of the Taiwan Central Weather Bureau one-tier (TCWB1T) fully coupled global atmospheric and oceanic modeling forecast system had been developed and implemented as a routine operation for seasonal prediction at Central Weather Bureau (CWB) in 2017, with a minor revision in 2020. Based on NCEP CFSv1, the global atmospheric model in NCEP CFSv1 was replaced by CWB’s atmospheric global spectral model (GSM) and coupled with the GFDL MOM3. Several parameters have been tested and tuned in the CWB atmospheric GSM, achieving an optimal configuration with better sea surface temperature (SST) predictions for integration more than one year. Using NCEP CFSR as the initial condition, TCWB1T conducted hindcasts from 1982 to 2011 and forecasts from 2012 to 2019 to analyze its performance. The results of these hindcasts and forecasts show that the TCWB1T can make useful predictions as verified against the observations of OISST, ERSST, CFSR, and GPCP based on the methods of EOF, RMSE, anomaly correlation, ranked probability skill score (RPSS), reliability diagram (RD), and relative operating characteristics (ROCs). TCWB1T also has the same level of skill scores as NCEP CFSv2 and/or the ECMWF fifth-generation seasonal forecast system (SEAS5), based on EOF, anomaly pattern correlation, climatological bias, RMSE, temporal correlation, and anomaly correlation percentage of forecast skill. TCWB1T shows forecast skill that is better in winter than in summer. Overall, it indicates that TCWB1T can be used for seasonal ENSO predictions.
Steering Flow Sensitivity in Forecast Models for Hurricane Ian (2022)
Authors: Colby Frank P. , Barlow Mathew , Penny Andrew B.
Published: March 2024

Hurricane Ian made landfall along Florida’s west coast at 1905 UTC 28 September 2022 near the Fort Myers area as a high-impact storm. Here, we examine the potential link between track forecast errors near the time of landfall and errors in both the synoptic-scale upper-level flow and a shortwave moving within that flow. Five days before the actual landfall (0000 UTC 23 September), most model guidance indicated landfall would occur close to where Ian eventually came ashore. But by 0000 UTC 25 September, model forecasts were all forecasting landfall in the Florida Panhandle. One day later, the models again agreed with each other but for a landfall 100–200 km north of Tampa, Florida. By 0000 UTC 27 September, forecast models indicated landfall would occur near Tampa. Model forecasts continued shifting to the right and finally converged on Punta Gorda, Florida, as the landfall location, less than 24 h before landfall. In this short article, we hypothesize that the track of Ian depended on subtle interactions with an extratropical wave in the middle and upper atmosphere. Deterministic and ensemble model forecasts reveal that the interactions were very sensitive to the characteristics of this wave and the synoptic-scale flow in which the wave was embedded. A 1–2-dam difference in the geopotential heights played a major role in whether Ian moved north into the Panhandle or toward the east, making landfall in central Florida.

Significance Statement

The purpose of this work is to look at possible causes of error in forecasts of Hurricane Ian’s landfall with the intent of saving lives and reducing damage, which can run to the billions of dollars. The path that hurricanes take is strongly influenced by the larger-scale weather patterns around them. We find that small errors in the upper-level flow patterns north of the hurricane appear to have played an important role in the forecast. These errors were present in both the general flow and a wave moving through that flow, resulting in small but significant changes to the forecast path. Understanding the cause of forecast errors is a key step both in improving forecasts and in identifying types of events that are more difficult to forecast.
Advanced Sea Ice Modeling for Short-Term Forecasting for Alaska’s Coasts
Authors: Fujisaki-Manome Ayumi , Hu Haoguo , Wang Jia , Westerink Joannes J. , Wirasaet Damrongsak , Ling Guoming , Choi Mindo , Moghimi Saeed , Myers Edward , Abdolali Ali , Dawson Clint , Janzen Carol
Published: March 2024

In Alaska’s coastal environment, accurate information of sea ice conditions is desired by operational forecasters, emergency managers, and responders. Complicated interactions among atmosphere, waves, ocean circulation, and sea ice collectively impact the ice conditions, intensity of storm surges, and flooding, making accurate predictions challenging. A collaborative work to build the Alaska Coastal Ocean Forecast System established an integrated storm surge, wave, and sea ice model system for the coasts of Alaska, where the verified model components are linked using the Earth System Modeling Framework and the National Unified Operational Prediction Capability. We present the verification of the sea ice model component based on the Los Alamos Sea Ice Model, version 6. The regional, high-resolution (3 km) configuration of the model was forced by operational atmospheric and ocean model outputs. Extensive numerical experiments were conducted from December 2018 to August 2020 to verify the model’s capability to represent detailed nearshore and offshore sea ice behavior, including landfast ice, ice thickness, and evolution of air–ice drag coefficient. Comparisons of the hindcast simulations with the observations of ice extent presented the model’s comparable performance with the Global Ocean Forecast System 3.1 (GOFS3.1). The model’s skill in reproducing landfast ice area significantly outperformed GOFS3.1. Comparison of the modeled sea ice freeboard with the Ice, Cloud, and Land Elevation Satellite-2 product showed a mean bias of −4.6 cm. Daily 5-day forecast simulations for October 2020–August 2021 presented the model’s promising performance for future implementation in the coupled model system.

Significance Statement

Accurate sea ice information along Alaska’s coasts is desired by the communities for preparedness of hazardous events, such as storm surges and flooding. However, such information, in particular predicted conditions, remains to be a gap. This study presents the verification of the state-of-art sea ice model for Alaska’s coasts for future use in the more comprehensive coupled model system where ocean circulation, wave, and sea ice models are integrated. The model demonstrates comparable performance with the existing operational ocean–ice coupled model product in reproducing overall sea ice extent and significantly outperformed it in reproducing landfast ice cover. Comparison with the novel satellite product presented the model’s ability to capture sea ice freeboard in the stable ice season.
Safety and Efficacy of Deep Transcranial Magnetic Stimulation for Management of Emotional Dysregulation in Children and Adolescents with Externalizing Behavior Disorders: Protocol of a Transdiagnostic Sham Controlled fMRI Study
Authors: Shukla Devangi , Sharma Pooja , Roy Chandramouli , Goyal Nishant
Published: March 2024

Background:

High-frequency deep transcranial magnetic stimulation (dTMS) on the anterior cingulate cortex (ACC) and medial prefrontal cortex (mPFC) has been known to be effective in modulating emotional experience but not studied in children and adolescents with externalizing behavior disorders (EBDs). We present a novel protocol for a study that aims to assess the safety and efficacy of adjuvant dTMS in managing emotional dysregulation in EBDs in children and adolescents.

Methods:

The trial is prospectively registered in the Clinical Trial Registry of India (CTRI) at www.ctri.nic.in with registration number: CTRI/2023/03/050701. In total, 40 subjects with age less than 18 years with EBDs would be randomized into two groups (active and sham dTMS); receiving 15 sessions of high-frequency dTMS, each, over 3 weeks. The subjects and rater would remain blind to treatment allocation. Assessments would be done at baseline and immediately after completion of the treatment using the Child Behavior Checklist (CBCL), Difficulty in Emotional Regulation Scale (DERS), Modified Overt Aggression Scale (MOAS), Affective Reactivity Index (ARI), Barratt’s Impulsivity Scale (BIS), Drug Abuse Screening Test (DAST), Children Global Assessment Scale (CGAS), and Clinical Global Impression (CGI). A checklist for side effects will be administered following each session in both groups.

Result:

Data shall be analyzed utilizing the statistical software Statistical Package for Social Sciences for outcome variables as defined for the purpose of the study. Safety of dTMS in young subjects as assessed by TMSens_Q and reduction in scores of DERS would be primary outcome variables. Functional Magnetic Resonance Imaging (fMRI) task-based assessment of the difference in activation of mPFC and ACC at baseline and after application of dTMS and reduction in scores of BIS, ARI, MOAS, CGI, and CGAS would be measured as secondary outcome variables.

Conclusion:

The study’s results are going to provide insight into potential role of dTMS in addressing emotional dysregulation in EBDs in children and adolescents adding one more tool to the armamentarium.
Intravenous iron infusions in pediatric patients: A retrospective review of efficacy and safety
Authors: Strachan Caitlin , Kugler Emmalee , Devgan Kartik , Nestor Jennifer , Afridi Faraz , Raju Riya , Hunter Krystal , Ahmed Rafat
Published: March 2024

Pediatric iron deficiency anemia (IDA) is often treated with oral iron supplementation as the first-line therapy despite poor adherence. This single-institution retrospective chart review of pediatric patients was conducted to assess the safety, efficacy, and adherence of intravenous (IV) iron infusions compared to oral iron therapy in patients who had failed a trial of oral iron supplementation. We reviewed medical records of patients aged 1–21 with IDA who received at least one IV iron infusion at Cooper University Hospital between 2016 and 2021. Paired t-tests compared pre-infusion and post-infusion hematologic indices of hemoglobin (Hgb), mean corpuscular volume, red blood cell count, red cell distribution width, ferritin, total iron binding capacity, iron stores, and iron saturation. We compared adherence and adverse reactions to both oral iron supplementation and IV iron infusions using McNemar’s test. A total of 107 subjects were included (mean age of 12.7 years). Hgb, ferritin, iron, and iron saturation between pre-infusion and post-final infusion significantly improved (p < 0.001). Hgb, ferritin, and iron improved when subcategorizing by race and etiology of IDA. Adherence to IV iron infusions (70.1%) was significantly greater than adherence to oral iron therapy (43.0%). There were also significantly fewer adverse effects with IV iron infusions (3.7%) compared to oral iron (77.9%). We demonstrated the safety, efficacy, and improved adherence of IV iron infusions compared to oral iron supplementation for treatment of pediatric IDA in patients who were unable to tolerate oral iron supplementation. Future studies could compare adherence to multiple doses of IV iron infusions in contrast with other single-dosing IV iron formulations.
Impact of SARS-COV-2 Infection on Maternal, Obstetric and Neonatal Outcomes in a Cohort of Vaccinated Women: A Pilot Study
Authors: Cruz-Calvente María , Rueda-Medina Blanca , Gil-Gutiérrez Rocío , Medina-Martínez Irene , Gómez-Urquiza José L. , Correa-Rodríguez María
Published: March 2024

We aimed to investigate the impact of COVID-19 infection on maternal characteristics and obstetric and neonatal outcomes in a cohort of women in labor previously vaccinated who tested positive for SARS-CoV-2 infection, compared to aged-matched healthy controls. A retrospective case-control study was conducted among 66 women in labor. Clinical data were obtained from medical records. The attendance rates at childbirth and parenting classes, as well as the implementation of a birth plan, were significantly lower in the COVID-19 infection group (6.1% vs. 48.5%, <0.001; 6.1% vs. 33.3%, p = .005, respectively). Women with COVID-19 had a higher prevalence of prolonged postpartum hospital stay (33.3% vs. 9.1%, p = .016), and significantly higher prevalence of spontaneous preterm birth (27.3% vs. 1.09%, p = .006). Breastfeeding within the first 24 hr was also lower in women with COVID-19 (72.7% vs. 97.0%, p = .006). Maternal characteristics and neonatal outcomes are influenced by COVID-19 infection in vaccinated women. Complications include spontaneous preterm birth, prolonged postpartum hospital stay, and lack of breastfeeding within the first 24 hr. Childbirth education, parenting classes and implementing a birth plan may be associated with a decreased risk of COVID-19 infection.
An intergenerational comeback: Girls’ education, development, and social capital
Authors: Peng Sheng-Hsiang Lance
Published: March 2024

This article examines the trajectory of global human rights expansion, with a specific focus on the advancement of girls’ and women’s education. By adopting a generational lens and using a reflective standpoint, I unpack the role of gender-specific social capital in fostering agency and empowerment among girls, particularly within the Global South. Building upon Porter’s Girls’ education, development and social change: ‘Seeding, Strengthening and Linking’ (Global Fund for Women) (2016), I propose a civic pedagogical PCCS (Place-based education initiatives, Cost-efficient and ecologically sound innovations, Collective intelligence building, Shared knowledge inquiry) model as a response to humanitarian crises, ideological disparities, and armed conflicts. Additionally, I draw upon the Indian concept of jugaad to comprehend the social, spatial, and economic negotiations in the Global South, enabling strategic resourcefulness. Furthermore, I introduce a Dalitbahujan feminist lens to emphasise the significance of girls’ education in the current decade and beyond. By reexamining Porter’s narratives and considering global circumstances, I focus on the dynamics of gender-oriented social capital. This article enriches the discussion on girls’ education within the frameworks of 21st-century ideologies of neoliberalism, philanthrocapitalism, and neo-capitalism, highlighting the crucial role of collaborative efforts.
A fragile effect: The influence of episodic memory on delay discounting
Authors: Duff Nicky , Olsen Rebecca , Walsh Zoe , Salmon Karen , Hunt Maree , Macaskill Anne
Published: March 2024

Delay discounting occurs when a reward loses value as a function of delay. Episodic future thinking (EFT) reliably decreases delay discounting. EFT may share cognitive features with recalling episodic memories such as constructive episodic simulation. We therefore explored whether recalling episodic memories also reduces delay discounting. In Experiment 1, participants wrote about episodic memories and recalled those memories before completing a delay discounting task. Episodic memories reduced delay discounting according to one commonly used delay discounting measure (area under the curve) but not another (using the hyperbolic model). Experiment 2 compared the effects of general and episodic memories. Neither general nor episodic memories significantly decreased delay discounting compared with a control “counting” condition, but episodic memories reduced delay discounting compared with general memories under some conditions. In Experiment 3, episodic memories did not decrease delay discounting compared with three other control conditions while EFT did. Experiment 3 therefore found that thinking must be both episodic and future orientated to reduce delay discounting. Together, these results suggest that episodic thinking is not sufficient to reliably decrease delay discounting, rather, features unique to episodic future thinking are required. Episodic memory might reduce delay discounting in some contexts, but this effect is small and fragile.
Edoxaban for the treatment of hypercoagulability and cerebral thromboembolism associated with cancer: A randomized clinical trial of biomarker targets
Authors: Chung Jong-Won , Hwang Jaechun , Kim Hyung Jun , Seo Woo-Keun , Ahn Myung-Ju , Saver Jeffrey L , Bang Oh Young
Published: March 2024

Background:

This study aimed compare efficacy of edoxaban and enoxaparin upon biomarkers of hypercoagulability in patients with cancer-related embolic stroke of undetermined source (ESUS).

Methods:

In this open-label, randomized, pilot trial, patients with cancer-related ESUS within 30 days of diagnosis were randomly assigned (1:1) to receive edoxaban (60 mg once daily) or enoxaparin (1 mg/kg twice daily) for 90 days. The primary endpoint was interval change of serum D-dimer level between days 0 and 7. The secondary endpoints were microembolic signals detected by transcranial Doppler at 7 and 90 days, the modified Rankin scale score, and stroke recurrence during 90 days. Safety outcomes included major bleeding and all-cause death at 90 days.

Results:

Of 303 patients with ischemic stroke and cancer, 40 fully met enrollment criteria and were randomized. Baseline D-dimer levels were numerically higher in the edoxaban group (22.9 ± 15.9 μg/mL vs 16.9 ± 16.9 μg/mL). D-dimer level change (%) between days 0 and 7 was similar in the two groups (53.2 ± 25.7 vs 52.2 ± 52.0; P = 0.11). Microembolic signals were detected in 41.1% and 43.8% at baseline, 41.2% and 42.9% at day 7, and 25.0% and 28.6% at day 90 in the edoxaban and enoxaparin groups, respectively. Non-significantly higher major bleeding (35.0% vs 10.0%, P = 0.06) and 90-day mortality (40.0% vs 25.0%, P = 0.31) were noted in the edoxaban group.

Conclusion:

Edoxaban and enoxaparin were comparable with respect to the biomarkers of hypercoagulability and cerebral thromboembolism. Larger trials are warranted to compare effects of edoxaban and enoxaparin upon recurrent stroke and major bleeding in patients with cancer-related ESUS.

Trial registration:

clinicaltrials.gov Identifier: NCT03570281 ( https://clinicaltrials.gov/ct2/show/NCT03570281 )
Association of LDL-cholesterol <1.8 mmol/L and statin use with the recurrence of intracerebral hemorrhage
Authors: Lee Tsz-Ching , Leung William CY , Ho Chun , Chiu Megan WL , Leung Ian YH , Wong Yuen-Kwun , Roxanna Liu KC , Sum Christopher HF , Lui David TW , Cheung Raymond TF , Leung Gilberto KK , Chan Koon-Ho , Teo Kay-Cheong , Lau Kui-Kai
Published: March 2024

Background:

Recent intensive low-density lipoprotein cholesterol (LDL-C) lowering trials, including FOURIER, ODYSSEY OUTCOMES, and Treat Stroke to Target (TST) trials, have mostly refuted the concern surrounding statin use, LDL-C lowering, and intracerebral hemorrhage (ICH) risk. However, the results from these trials may not be fully applied to ICH survivors, as the populations studied were mainly patients without prior ICH, in whom the inherent ICH risk is more than 10 times lower than that of ICH survivors. Although available literature on statin use after ICH has demonstrated no excess risk of recurrent ICH, other potential factors that may modify ICH risk, especially hypertension control and ICH etiology, have not generally been considered. Notably, data on LDL-C levels following ICH are lacking.

Aims:

We aim to investigate the association between LDL-C levels and statin use with ICH risk among ICH survivors, and to determine whether the risk differed with patients’ characteristics, especially ICH etiology.

Methods:

Follow-up data of consecutive spontaneous ICH survivors enrolled in the University of Hong Kong prospective stroke registry from 2011 to 2019 were retrospectively analyzed. ICH etiology was classified as cerebral amyloid angiopathy (CAA) using the modified Boston criteria or hypertensive arteriopathy, while the mean follow-up LDL-C value was categorized as <1.8 or ⩾1.8 mmol/L. The primary endpoint was recurrent ICH. The association of LDL-C level and statin use with recurrent ICH was determined using multivariable Cox regression. Pre-specified subgroup analyses were performed, including based on ICH etiology and statin prescription. Follow-up blood pressure was included in all the regression models.

Results:

In 502 ICH survivors (mean age = 64.2 ± 13.5 years, mean follow-up LDL-C = 2.2 ± 0.6 mmol/L, 28% with LDL-C <1.8 mmol/L), 44 had ICH recurrence during a mean follow-up of 5.9 ± 2.8 years. Statin use after ICH was not associated with recurrent ICH (adjusted hazard ratio (AHR) = 1.07, 95% confidence interval (CI) = 0.57–2.00). The risk of ICH recurrence was increased for follow-up LDL-C <1.8 mmol/L (AHR = 1.99, 95% CI = 1.06–3.73). This association was predominantly observed in ICH attributable to CAA (AHR = 2.52, 95% CI = 1.06–5.99) and non-statin users (AHR = 2.91, 95% CI = 1.08–7.86).

Conclusion:

The association between post-ICH LDL-C <1.8 mmol/L and recurrent ICH was predominantly observed in CAA patients and those with intrinsically low LDL-C (non-statin users). While statins can be safely prescribed in ICH survivors, LDL-C targets should be individualized and caution must be exercised in CAA patients.
Immobilisation of chromium in magnesium carbonate minerals
Authors: Lacinska Alicja M. , Bateman Keith , Chenery Simon , Kemp Simon J , Liddy Thomas , Rushton Jeremy C , Saha Dipankar , Schroeder Sven L.M.
Published: March 2024

Hexavalent chromium (Cr6+) is a toxic carcinogenic pollutant that might be released by the mining and processing of ultramafic rocks and nickel laterites and which requires permanent removal from the contaminated biosphere. Ultramafic material can also serve as a feedstock for the sequestration of CO2 resulting from the growth of new minerals, raising the intriguing proposition of integrated sequestration of both pollutants, CO2 and chromium, into magnesium carbonates. Such a synergistic process downstream of ore recovery and mineral processing could be an elegant proposition for more sustainable utilisation and management of the Earth's resources. We have therefore carried out an experimental and microanalytical study to investigate potentially suitable carbonate minerals. Uptake of chromium in carbonate phases was determined, followed by identification of the crystalline phases and characterisation of the local structural environment around the incorporated chromium centres. The results suggest that neither nesquehonite nor hydromagnesite have the structural capacity to incorporate Cr6+ or Cr3+ significantly at room temperature. We therefore propose that further research into this technology should focus on laboratory assessments of other phases, such as layered double hyroxides, that have a natural structural capacity to uptake both chromium and CO2.
Differential effects of itacitinib, fedratinib, and ruxolitinib in mouse models of hemophagocytic lymphohistiocytosis
Authors: Keenan Camille , Albeituni Sabrin , Oak Ninad , Stroh Alexa , Tillman Heather S. , Wang Yingzhe , Freeman Burgess B. , Alemán-Arteaga Silvia , Meyer Lauren K. , Woods Rolanda , Verbist Katherine C. , Zhou Yinmei , Cheng Cheng , Nichols Kim E.
Published: March 2024

Hemophagocytic lymphohistiocytosis (HLH) comprises a severe hyperinflammatory phenotype driven by the overproduction of cytokines, many of which signal via the JAK/STAT pathway. Indeed, the JAK1/2 inhibitor ruxolitinib has demonstrated efficacy in preclinical studies and early-phase clinical trials in HLH. Nevertheless, concerns remain for ruxolitinib-induced cytopenias, which are postulated to result from the blockade of JAK2-dependent hematopoietic growth factors. To explore the therapeutic effects of selective JAK inhibition in mouse models of HLH, we carried out studies incorporating the JAK1 inhibitor itacitinib, JAK2 inhibitor fedratinib, and JAK1/2 inhibitor ruxolitinib. All 3 drugs were well-tolerated and at the doses tested, they suppressed interferon-gamma (IFN-γ)–induced STAT1 phosphorylation in vitro and in vivo. Itacitinib, but not fedratinib, significantly improved survival and clinical scores in CpG–induced secondary HLH. Conversely, in primary HLH, in which perforin-deficient (Prf1−/−) mice are infected with lymphocytic choriomeningitis virus (LCMV), itacitinib, and fedratinib performed suboptimally. Ruxolitinib demonstrated excellent clinical efficacy in both HLH models. RNA-sequencing of splenocytes from LCMV-infected Prf1−/− mice revealed that itacitinib targeted inflammatory and metabolic pathway genes in CD8 T cells, whereas fedratinib targeted genes regulating cell proliferation and metabolism. In monocytes, neither drug conferred major transcriptional impacts. Consistent with its superior clinical effects, ruxolitinib exerted the greatest transcriptional changes in CD8 T cells and monocytes, targeting more genes across several biologic pathways, most notably JAK-dependent proinflammatory signaling. We conclude that JAK1 inhibition is sufficient to curtail CpG-induced disease, but combined inhibition of JAK1 and JAK2 is needed to best control LCMV-induced immunopathology.
Pooled analysis of pralatrexate single-agent studies in patients with relapsed/refractory peripheral T-cell lymphoma
Authors: O’Connor Owen A. , Ko Bor-Sheng , Wang Ming-Chung , Maruyama Dai , Song Yuqin , Yeoh Ee-Min , Manamley Nick , Tobinai Kensei
Published: March 2024

Patients with relapsed or refractory (R/R) mature natural killer cell and T-cell lymphoma have limited treatment options. To evaluate pralatrexate’s performance and factors influencing its safety and efficacy in R/R peripheral T-cell lymphoma (PTCL), we performed a pooled analysis of data from 4 similarly designed, regulatory-mandated prospective clinical trials. Of 221 patients (median age, 59 years; 67.0% male) in the study population, 48.9% had PTCL not otherwise specified (PTCL-NOS), 21.3% angioimmunoblastic T-cell lymphoma, and 11.8% ALK-negative anaplastic large cell lymphoma (ALCL). Patients received pralatrexate for a median of 2.56 months (range, 0.03-24.18) and had a 40.7% objective response rate with a median duration of response of 9.1 months, progression-free survival 4.6 months, and overall survival 16.3 months. The most common treatment-related all-grade adverse events were stomatitis, thrombocytopenia, white blood cell count decrease, pyrexia, and vomiting. Subgroup exploratory analyses suggest improved efficacy with 1 prior line of chemotherapy vs 2 or ≥4 prior lines; PTCL-NOS or ALCL vs transformed mycosis fungoides; chemotherapy and transplant before pralatrexate vs chemotherapy alone or chemotherapy with other nontransplant treatments. In conclusion, these pooled analysis results further support using pralatrexate in patients with R/R PTCL. Prospective studies are needed to confirm the findings of subgroups analyses.
The impact of vitamin D on type 2 diabetes management: boosting PTP1B gene expression and physical activity benefits in rats
Authors: Khaledi Kimya , Hoseini Rastegar , Gharzi Ahmad
Published: March 2024

Background

The protein tyrosine phosphatase 1B (PTP1B) plays a crucial role in the development of insulin resistance. Aerobic training (AT) and vitamin D (Vit D) supplementation have been shown to individually improve glucose tolerance and diabetes-related factors. However, the impact of their combined effect on PTP1B gene expression and serum irisin in the visceral adipose tissue remains unknown. This study aims to investigate whether 8 weeks of combined AT with Vit D supplementation can improve the expression of PTP1B in adipose tissue and serum irisin in obese rats with type 2 diabetes (T2D).

Methods

Fifty male Wistar rats were divided into two groups: diabetic (n = 40) and non-diabetic (ND; n = 10). The diabetic rats were further divided into four groups: aerobic training with vitamin D supplementation (D + AT + Vit D; n = 10), aerobic training only (D + AT; n = 10), vitamin D supplementation only (D + Vit D; n = 10), and control (D + C; n = 10). The D + Vit D and D + AT + Vit D groups received 5000 IU of vitamin D via injection once a week, while the D + AT and D + C groups received sesame oil. Diabetes was induced in all groups except the nondiabetic group by intraperitoneal (IP) injection of streptozotocin. At the end of the intervention, blood and adipose tissue samples were collected, and RNA was extracted from adipose tissue for real-time PCR analysis of PPTP1B gene expression.

Results

There was an increase in serum Vit D and irisin levels and a decrease in HOMA-IR and PTP1B gene expression in the diabetic rat model treated with D + AT and injected with 50,000 IU/kg/week of Vit D. Comparatively, when treated with D + AT + Vit D, the downregulation of PTP1B was significantly higher (p = 0.049; p = 0.004), and there was a significant increase in irisin (p = 0.010; p = 0.001).

Conclusion

The present study shows that the combined AT and Vit D supplementation positively impacts the expression of PTP1B in adipose tissue and serum irisin in rats with T2D. These findings suggest that combining AT with Vit D supplementation can provide a new and effective strategy to improve glucose tolerance and diabetes-related factors in individuals with T2D by regulating the expression of PTP1B in adipose tissue and promoting the synthesis of beneficial irisin protein.
A chromosome level genome assembly of Pseudoroegneria Libanotica reveals a key Kcs gene involves in the cuticular wax elongation for drought resistance
Authors: Zhai Xingguang , Wu Dandan , Chen Chen , Yang Xunzhe , Cheng Shaobo , Sha Lina , Deng Shuhan , Cheng Yiran , Fan Xing , Kang Houyang , Wang Yi , Liu Dengcai , Zhou Yonghong , Zhang Haiqin
Published: March 2024

Background

The genus Pseudoroegneria (Nevski) Löve (Triticeae, Poaceae), whose genome symbol was designed as “St”, accounts for more than 60% of perennial Triticeae species. The diploid species Psudoroegneria libanotica (2n = 14) contains the most ancient St genome, exhibited strong drought resistance, and was morphologically covered by cuticular wax on the aerial part. Therefore, the St-genome sequencing data could provide fundamental information for studies of genome evolution and reveal its mechanisms of cuticular wax and drought resistance.

Results

In this study, we reported the chromosome-level genome assembly for the St genome of Pse. libanotica, with a total size of 2.99 Gb. 46,369 protein-coding genes annotated and 71.62% was repeat sequences. Comparative analyses revealed that the genus Pseudoroegneria diverged during the middle and late Miocene. During this period, unique genes, gene family expansion, and contraction in Pse. libanotica were enriched in biotic and abiotic stresses, such as fatty acid biosynthesis which may greatly contribute to its drought adaption. Furthermore, we investigated genes associated with the cuticular wax formation and water deficit and found a new Kcs gene evm.TU.CTG175.54. It plays a critical role in the very long chain fatty acid (VLCFA) elongation from C18 to C26 in Pse. libanotica. The function needs more evidence to be verified.

Conclusions

We sequenced and assembled the St genome in Triticeae and discovered a new KCS gene that plays a role in wax extension to cope with drought. Our study lays a foundation for the genome diversification of Triticeae species and deciphers cuticular wax formation genes involved in drought resistance.
Effect of hydrogen sulfide (H2S) on the growth and development of tobacco seedlings in absence of stress
Authors: Dai Jingcheng , Wen Dingxin , Li Hao , Yang Jingpeng , Rao Xiongfei , Yang Yong , Yang Jiangke , Yang Chunlei , Yu Jun
Published: March 2024

Background

Hydrogen sulfide (H2S) is a novel signaling molecule involved in the growth and development of plants and their response to stress. However, the involvement of H2S in promoting the growth and development of tobacco plants is still unclear.

Results

In this study, we explored the effect of pre-soaking or irrigating the roots of tobacco plants with 0.0, 2.0, 4.0, 6.0, and 8.0 mM of sodium hydrosulfide (NaHS) on endogenous H2S production, antioxidant enzymatic and cysteine desulfhydrase activities, seed germination, agronomic traits, photosynthetic pigments contents, and root vigor. The results revealed that exogenous NaHS treatment could significantly promote endogenous H2S production by inducing gene expression of D/L-CD and the activities of D/L-CD enzymes. Additionally, a significant increase in the agronomic traits and the contents of photosynthetic pigments, and no significant difference in carotenoid content among tobacco plants treated with 0.0 to 8.0 mM of NaHS was observed. Additionally, a significant increase in the germination speed, dry weight, and vigor of tobacco seeds, whereas no significant effect on the percentage of seed germination was observed on NaHS treatment. Furthermore, NaHS treatment could significantly increase the activity of superoxide dismutase (SOD) and peroxidase (POD) enzymes, which reduces damage due to oxidative stress by maintaining reactive oxygen species homeostasis.

Conclusions

These results would aid in enhancing our understanding of the involvement of H2S, a novel signaling molecule to promote the growth and development of tobacco plants.
Differential responses of two fenugreek (Trigonella foenum-graecum L.) landraces pretreated with melatonin to prolonged drought stress and subsequent recovery
Authors: Maleki Masoud , Shojaeiyan Abdolali , Mokhtassi-Bidgoli Ali
Published: March 2024

Background

Drought impairs growth, disturbs photosynthesis, and induces senescence in plants, which results in crop productivity reduction and ultimately jeopardizes human food security. The objective of this study was to determine major parameters associated with drought tolerance and recovery ability of fenugreek (Trigonella foenum-graecum L.), by examining differential biochemical and phenological responses and underlying enzyme activities as well as melatonin roles during drought stress and re-watering for two contrasting landraces. Moreover, the relative expression of three key genes involved in the biosynthesis pathway of diosgenin, including SQS, CAS, and BG, was investigated.

Results

Depending on the conditions, drought stress enhanced the activity of antioxidant enzymes and the osmoregulating compounds, non-enzymatic antioxidants, hydrogen peroxide content, and lipid peroxidation levels in most cases. Severe drought stress accelerated flowering time in Shushtar landrace (SHR) but had no significant effects on Varamin (VR). Pretreatment with melatonin delayed flowering time in SHR and caused high drought resistance in this landrace. Furthermore, melatonin significantly enhanced drought adaptability in VR by improving plant recovery ability.

Discussion

Based on our results plants’ responses to drought stress and melatonin pretreatment were completely landrace-specific. Drought stress caused an increase in the relative expression of CAS gene and ultimately the accumulation of steroidal saponins in SHR. Melatonin compensated for the decrease in biomass production due to drought stress and finally increased steroidal saponins performance in SHR. Our study showed that melatonin can improve drought stress and recovery in fenugreek, but different factors such as genotype, melatonin concentration, and plant age should be considered.
Prediction of preload dependency using phenylephrine-induced peripheral perfusion index during general anaesthesia: a prospective observational study
Authors: Iizuka Yusuke , Yoshinaga Koichi , Amitani Shizuka , Nishiyama Seiya , Fukano Kentaro , Miyazawa Keika , Kitajima Asuka , Sawada Ikumi , Otsuka Yuji , Sanui Masamitsu
Published: March 2024

Background

Tracking preload dependency non-invasively to maintain adequate tissue perfusion in the perioperative period can be challenging.The effect of phenylephrine on stroke volume is dependent upon preload. Changes in stroke volume induced by phenylephrine administration can be used to predict preload dependency. The change in the peripheral perfusion index derived from photoplethysmography signals reportedly corresponds with changes in stroke volume in situations such as body position changes in the operating room. Thus, the peripheral perfusion index can be used as a non-invasive potential alternative to stroke volume to predict preload dependency. Herein, we aimed to determine whether changes in perfusion index induced by the administration of phenylephrine could be used to predict preload dependency.

Methods

We conducted a prospective single-centre observational study. The haemodynamic parameters and perfusion index were recorded before and 1 and 2 min after administering 0.1 mg of phenylephrine during post-induction hypotension in patients scheduled to undergo surgery. Preload dependency was defined as a stroke volume variation of ≥ 12% before phenylephrine administration at a mean arterial pressure of < 65 mmHg. Patients were divided into four groups according to total peripheral resistance and preload dependency.

Results

Forty-two patients were included in this study. The stroke volume in patients with preload dependency (n = 23) increased after phenylephrine administration. However, phenylephrine administration did not impact the stroke volume in patients without preload dependency (n = 19). The perfusion index decreased regardless of preload dependency. The changes in the perfusion index after phenylephrine administration exhibited low accuracy for predicting preload dependency. Based on subgroup analysis, patients with high total peripheral resistance tended to exhibit increased stroke volume following phenylephrine administration, which was particularly prominent in patients with high total peripheral resistance and preload dependency.

Conclusion

The findings of the current study revealed that changes in the perfusion index induced by administering 0.1 mg of phenylephrine could not predict preload dependency. This may be attributed to the different phenylephrine-induced stroke volume patterns observed in patients according to the degree of total peripheral resistance and preload dependency.

Trial registration

University Hospital Medical Information Network (UMIN000049994 on 9/01/2023).
Bone mineral density in lower thoracic vertebra for osteoporosis diagnosis in older adults during CT lung cancer screening
Authors: Hu Nandong , Wang Miaomiao , Yang Meng , Chen Xin , Wang Jiangchuan , Xie Chao , Zhang Bin , Wang Zhongqiu , Chen Xiao
Published: March 2024

Background

Quantitative computed tomography (QCT)-based lumbar bone mineral density (LBMD) has been used to diagnose osteoporosis. This study explored the value of lower thoracic BMD (TBMD) in diagnosing osteoporosis in older adults during CT lung cancer screening.

Methods

This study included 751 subjects who underwent QCT scans with both LBMD and TBMD. 141 of them was selected for a validation. Osteoporosis was diagnosed based on LBMD using the ACR criteria (gold standard). TBMD thresholds were obtained using receiver operating characteristic curve. TBMD was also translated into LBMD (TTBMD) and osteoporosis was defined based on TTBMD using ACR criteria. The performance of TBMD and TTBMD in identifying osteoporosis was determined by Kappa test. The associations between TBMD- and TTBMD-based osteoporosis and fracture were tested in 227 subjects with followed up status of spine fracture.

Results

The performance of TBMD in identifying osteoporosis was low (kappa = 0.66) if using the ACR criteria. Two thresholds of TBMD for identifying osteopenia (128 mg/cm3) and osteoporosis (91 mg/cm3) were obtained with areas under the curve of 0.97 and 0.99, respectively. The performance of the identification of osteoporosis/osteopenia using the two thresholds or TTBMD both had good agreement with the gold standard (kappa = 0.78, 0.86). Similar results were observed in validation population. Osteoporosis identified using the thresholds (adjusted hazard ratio (HR) = 18.72, 95% confidence interval (CI): 5.13–68.36) or TTBMD (adjusted HR = 10.28, 95% CI: 4.22–25.08) were also associated with fractures.

Conclusion

Calculating the threshold of TBMD or normalizing TBMD to LBMD are both useful in identifying osteoporosis in older adults during CT lung cancer screening.