bluuebunny/crossref_metadata_2025 huggingface dataset:
Mechanisms underlying the role of endoplasmic reticulum stress in the placental injury and fetal growth restriction in an ovine gestation model
Authors: Zhang Hao , Zha Xia , Zheng Yi , Liu Xiaoyun , Elsabagh Mabrouk , Wang Hongrong , Jiang Honghua , Wang Mengzhi
Published: September 2023

Background

Exposure to bisphenol A (BPA), an environmental pollutant known for its endocrine-disrupting properties, during gestation has been reported to increase the risk of fetal growth restriction (FGR) in an ovine model of pregnancy. We hypothesized that the FGR results from the BPA-induced insufficiency and barrier dysfunction of the placenta, oxidative stress, inflammatory responses, autophagy and endoplasmic reticulum stress (ERS). However, precise mechanisms underlying the BPA-induced placental dysfunction, and subsequently, FGR, as well as the potential involvement of placental ERS in these complications, remain to be investigated.

Methods

In vivo experiment, 16 twin-pregnant (from d 40 to 130 of gestation) Hu ewes were randomly distributed into two groups (8 ewes each). One group served as a control and received corn oil once a day, whereas the other group received BPA (5 mg/kg/d as a subcutaneous injection). In vitro study, ovine trophoblast cells (OTCs) were exposed to 4 treatments, 6 replicates each. The OTCs were treated with 400 μmol/L BPA, 400 μmol/L BPA + 0.5 μg/mL tunicamycin (Tm; ERS activator), 400 μmol/L BPA + 1 μmol/L 4-phenyl butyric acid (4-PBA; ERS antagonist) and DMEM/F12 complete medium (control), for 24 h.

Results

In vivo experiments, pregnant Hu ewes receiving the BPA from 40 to 130 days of pregnancy experienced a decrease in placental efficiency, progesterone (P4) level and fetal weight, and an increase in placental estrogen (E2) level, together with barrier dysfunctions, OS, inflammatory responses, autophagy and ERS in type A cotyledons. In vitro experiment, the OTCs exposed to BPA for 24 h showed an increase in the E2 level and related protein and gene expressions of autophagy, ERS, pro-apoptosis and inflammatory response, and a decrease in the P4 level and the related protein and gene expressions of antioxidant, anti-apoptosis and barrier function. Moreover, treating the OTCs with Tm aggravated BPA-induced dysfunction of barrier and endocrine (the increased E2 level and decreased P4 level), OS, inflammatory responses, autophagy, and ERS. However, treating the OTCs with 4-PBA reversed the counteracted effects of Tm mentioned above.

Conclusions

In general, the results reveal that BPA exposure can cause ERS in the ovine placenta and OTCs, and ERS induction might aggravate BPA-induced dysfunction of the placental barrier and endocrine, OS, inflammatory responses, and autophagy. These data offer novel mechanistic insights into whether ERS is involved in BPA-mediated placental dysfunction and fetal development.
Cognitive flexibility and DSM-5 severity criteria for eating disorders: assessing drive for thinness and duration of illness as alternative severity variables
Authors: Mora-Maltas Bernat , Lucas Ignacio , Granero Roser , Vintró-Alcaraz Cristina , Miranda-Olivos Romina , Baenas Isabel , Sánchez Isabel , Jiménez-del Toro Jessica , Sánchez-González Jéssica , Krug Isabel , Tapia Javier , Jiménez-Murcia Susana , Fernández-Aranda Fernando
Published: September 2023

Background

The severity criteria for eating disorders (EDs) proposed in the DSM-5 have been established without sufficient empirical support. Drive for thinness (DT) and duration of illness have been proposed as two alternative severity measures, however their empirical evidence is also limited. To date, no research has assessed the validity of current eating disorder (ED) severity criteria regarding cognitive flexibility factors. Cognitive flexibility is often impaired in EDs, becoming a possible severity symptom. The current study assessed for the first time (1) whether the severity indexes for EDs proposed in the DSM-5 were associated with deficits in cognitive flexibility and, (2) whether drive for thinness and illness duration, acted as an alternative, more meaningful severity indices for deficiencies in cognitive flexibility.

Methods

Participants were 161 patients diagnosed with an ED, who were categorized according to DSM-5 severity categories, DT and duration of illness. Discriminative capacity of each classification was assessed for cognitive flexibility measured by Wisconsin card sorting test (WCST).

Results

The findings for the DSM-5 classification comprised: (a) In the anorexia nervosa (AN) group, patients with moderate severity showed better scores in WCST than patients with mild and severe/extreme severity. Also, patients with moderate severity showed lower percentage of cognitive flexibility deficits than the other two severity categories; (b) For the binge spectrum disorders (BSD) group, the patients with mild severity showed a higher percentage of cognitive flexibility deficits than did the moderate and severe/extreme categories. When assessing the alternative severity index of DT, no differences were found in cognitive flexibility in any of the groups. Regarding illness duration, in the AN group the task performance of the patients with longer illness duration was worse than the performance of the short duration group and, in the BSD group, patients with longer duration also showed more deficits in cognitive flexibility than the patients with shorter duration of illness.

Conclusions

Our findings point out the limitations of the DSM-5 severity criteria to categorize cognitive flexibility in EDs and support illness duration as an alternative severity approach for EDs.
Effects of family-based treatment on adolescent outpatients treated for anorexia nervosa in the Eating Disorder Unit of Helsinki University Hospital
Authors: Oshukova Svetlana , Suokas Jaana , Nordberg Mai , Ålgars Monica
Published: September 2023

Background

Family therapy for adolescent anorexia nervosa (AN) has stronger evidence of efficacy in comparison with individual therapy, and family-based treatment (FBT) is the most evaluated in numerous randomized clinical trials. However, few studies have focused on how FBT performs outside of research settings. The current study is the first to assess clinical outcomes of FBT for adolescent AN in Finland, in a specialized outpatient clinic.

Aim

The naturalistic outcome of outpatient FBT for adolescent AN was investigated.

Methods

Fifty-two female patients and their families who received FBT at a tertiary eating disorders unit participated in the study. Data on their pre-treatment parameters, treatment details, and condition at the end of treatment (EOT) was collected from their medical records.

Results

At EOT, a majority (61.5%) had achieved a full weight restoration [percentage of expected body weight (%EBW) ≥ 95%]. Participants with an %EBW ≥ 95 at EOT had a significantly higher pre-treatment %EBW than those with an EBW < 95% at EOT. Participants with an EBW ≥ 95% at EOT showed significantly higher total weight gain during the treatment period, a higher rate of regular menstrual periods at EOT, significantly lower rates of dietary restrictions, and less cognitive or behavioral symptoms of the eating disorder overall, compared to participants who did not achieve a normal body weight. In 22 cases (42.3%), there was no need for further treatment at the end of FBT. Participants who needed further treatment after FBT, compared to those who did not, showed significantly higher rates of psychiatric comorbidity, history of mental health treatment, and need for psychopharmacological treatment.

Conclusions

In this naturalistic study, and in line with previous studies, FBT for AN appeared to be an effective and sometimes sufficient intervention, especially for patients with milder weight deficit and less severe psychiatric comorbidities. The results show that FBT can be successfully implemented in Finland and suggest that training more ED clinicians in FBT would be beneficial.

Trial registration: The study was retrospectively registered on February 8th, 2023, in ClinicalTrials.gov Protocol Registration and Results System, identifier: NCT05734573.
Improved recovery of NO2 sensors using heterojunctions between transition metal dichalcogenides and ZnO nanoparticles
Authors: Wang Leilei , Choi Jungwook
Published: September 2023

The stable recovery of gas sensors is an important indicator for evaluating their performance. Hitherto, the use of external light sources and/or an increase in the operating temperature has been effective in improving the recovery rate of gas sensors. Herein, heterojunctions were formed between the two-dimensional transition metal dichalcogenide nanosheets and zero-dimensional ZnO nanoparticles to improve the recovery rate of a NO2 sensor. Scanning electron microscopy and Raman spectroscopy suggested a successful deposition of ZnO nanoparticles onto the MoS2 and WSe2 nanosheets. The sensing response to 10 ppm NO2 gas at 100 °C indicated that the heterojunction formed by ZnO and MoS2 or WSe2 successfully improved the recovery rate of the sensor by 11.87% and 19.44%, respectively, whereas the sensitivity remained constant. The proposed approach contributes to improving the performance of gas sensors.
Level of detail (LoD) geometric analysis of relief mapping employing 3D modeling via UAV images in cultural heritage studies
Authors: Ergun Bahadir , Sahin Cumhur , Bilucan Furkan
Published: September 2023

Unmanned Aerial Vehicles (UAVs) are often preferred for modeling cultural heritage buildings due to their costs and time savings. The need for data collection, analysis, processing, and visual presentation in the context of cultural heritage buildings has become prominent, underscoring the significance of the concept of Level of Detail (LoD). The utilization of CityGML LoD standards enhances the performance of visual presentations, decreases the geometric complexity of objects, and enables users to view the model at the desired level of detail within a computerized environment. Within the scope of this study, it is aimed to determine the accuracy analysis of the 3D model for a cultural building, which is named Hekimbaşı Hunting Lodge, at different LoDs. Drawings were created at LoD 0-1-2-3 levels with 418 photographs taken by the UAV photogrammetry method. Additionally, conservative and UAV measures of facade detail at the same LoD were compared in terms of accuracy. As a result, RMSE values for X, Y, and Z axes at LoD3 standard were calculated as 1.394 cm, 0.861 cm, and 0.694 cm, respectively. It has been concluded that the high-accuracy LoD models for the cultural building could be produced using the UAV photogrammetry method at the desired accuracy.
The role of media in influencing students’ STEM career interest
Authors: Chen Chen , Hardjo Stephanie , Sonnert Gerhard , Hui Jiaojiao , Sadler Philip M.
Published: September 2023

Background

Digital media are pervasive in the lives of young people and provide opportunities for them to learn about STEM. Multiple theories argue that the STEM media environment may shape how youth see a STEM career in their future. Yet, little is known about how pre-college digital media consumption may be related to students’ STEM career interest at the beginning of college. The wide variety of STEM media also raises the question of potentially different effects and pathways by media type. In this study, we collected a nationally representative sample of more than 15,000 students in their first year in U.S. colleges and universities. We asked about their career interests at the beginning of college and also asked them to retrospectively report their STEM media consumption during high school.

Results

We found that watching STEM-related TV and online videos, as well as playing STEM-related video games during high school, were positively associated with students’ STEM career interests at the beginning of college. However, we also found that STEM media consumption did not impact directly on STEM career interest, but acted through two intermediaries: STEM identity (I and others see me as a STEM person) and three personal career outcome expectations: a high interest in self-development (enhancement and use of talents), and low interests in material status (money, fame, power) and in interpersonal relationships (helping, and working with, other people).

Conclusions

This study finds that STEM media have a significant effect in fostering STEM career interest, with most of the effect coming from STEM TV, STEM video viewing, and STEM video games. The effect is mediated mainly through students’ identity and, to a lesser extent, through personal values, such as self-development, material, and interpersonal relationship values. This study suggests that media communication should be mindful of how different platforms may deliver nuanced and varied messages of what STEM careers may afford and who can succeed in STEM.
Seismic noise between 0.003 Hz and 1.0 Hz and its classification
Authors: Tanimoto Toshiro , Anderson Aaron
Published: September 2023

It is now established that the primary microseism, the secondary microseisms, and the hum are the three main components of seismic noise in the frequency band from about 0.003 Hz to 1.0 Hz. Monthly averages of seismic noise are dominated by these signals in seismic noise. There are, however, some temporary additional signals in the same frequency band, such as signals from tropical cyclones (hurricanes and typhoons) in the ocean and on land, stormquakes, weather bombs, tornadoes, and wind-related atmospheric pressure loading. We review these effects, lasting only from a few hours to a week but are significant signals. We also attempt to classify all seismic noise. We point out that there are two broad types of seismic noise, the propagating seismic waves and the quasi-static deformations. The latter type is observed only for surface pressure changes at close distances. It has been known since about 1970 but has not been emphasized in recent literature. Recent data based on co-located pressure and seismic instruments clearly show its existence. Because the number of phenomena in the first type is large, we propose to classify all seismic noise into three categories: (1) propagating seismic waves from ocean sources, (2) propagating seismic waves from on-land sources, and (3) quasi-static deformation at ocean bottom and on land. The microseisms and the hum are in the first category although there are differences in the detailed processes of their excitation mechanisms. We will also classify temporary signals by these categories.
Historical and current knowledge of the Magellanic tuco-tuco Ctenomys magellanicus in Patagonia and Tierra del Fuego, South America
Authors: Jaksic Fabian M.
Published: September 2023

Background

I review the natural history of the tuco-tucoCtenomys magellanicusin Patagonia and Tierra del Fuego, southernmost geographic regions shared by Argentina and Chile. By natural history I mean both historical records and ecological features of this gregarious burrowing rodent.

Methods and results

I conducted a selective review of the natural history ofC. magellanicus, as recorded in mainstream journals and proceedings, landmark monographs and books, and internet sources. I report results on: (a) Original description, (b) Diversity and distribution, (c) Life history, (d) Interactions with plants and herbivorous competitors, (e) Interactions with predators other than humans, (f) Interactions with humans, and (g) Abundance and conservation. I also enter the anthropology field, but I only touch the archeology field. Although this review is not on the systematics ofC. magellanicus, I do provide an updated overview of the phylogenetics-based taxonomy of the genus where it belongs.

Discussion

There is a rather large body of literature onC. magellanicus,comprising the fields of anthropology, archeology, biogeography, biology, ecology, and systematics. These areas are broadly covered but thinly treated, offering interesting opportunities for further research. For instance, on adaptation, evolution, and environmental impact of the southernmost burrowing rodent in the world and its cultural impact on vanishing native peoples, especially the Selk’nam.
Successful conservative treatment for massive tracheal necrosis after lung segmentectomy
Authors: Tsubokawa Norifumi , Mimura Takeshi , Tadokoro Kazuki , Yamashita Yoshinori
Published: September 2023

Background

Tracheal necrosis, which is rare because the trachea has rich in blood supply, can be a serious condition. Herein, we report the case of extensive tracheal necrosis that developed after right apical segmentectomy for a metastatic lung tumor of esophageal cancer.

Case presentation

A 74-year-old man who had undergone thoracoscopic subtotal esophagectomy and gastric tube reconstruction via the posterior sternal route for esophageal adenocarcinoma 2 years previously was referred to our department with an enlarging nodal lesion in the right upper lung lobe. Computed tomography revealed a 30-mm tumor in the right apical segment with no lymph node enhancement, suggesting primary lung cancer or a metastatic lung tumor. The patient underwent right apical segmentectomy. The upper lobe was adherent to the chest wall and mediastinal fat from the apex of the lung to the dorsal side, with particularly strong adhesion at the esophagectomy site. After dissecting the adhesions, right apical segmentectomy was performed via complete video-assisted thoracic surgery. The patient was discharged on the 9th day after surgery without any complications. Pathologic findings revealed a metastatic lung tumor originating from the patient’s esophageal cancer. On the 26th day after surgery, the patient returned with dyspnea and increased sputum. Computed tomography images revealed that the posterior wall of the trachea was missing an area of 16 × 42 mm and was connected to the dead space after the right apical segmentectomy, with no effusion. We diagnosed extensive tracheal necrosis. Considering that the patient’s status was very well despite the extensive tracheal necrosis, we chose conservative treatment. After receiving 12 days of intravenous antibiotic treatment, his symptoms improved, and he was discharged on day 26 after admission.

Conclusions

Right upper lung lobe resection after esophagectomy has a risk of tracheal necrosis. Conservative treatment is one approach to manage massive tracheal necrosis in patients with stable respiratory conditions.
Prevalence of metabolic syndrome and components in rural, semi-urban and urban areas in the littoral region in Cameroon: impact of physical activity
Authors: Bilog Nadine Carole , Mekoulou Ndongo Jerson , Bika Lele Elysée Claude , Guessogo Wiliam Richard , Assomo-Ndemba Peguy Brice , Ahmadou  , Etaga Noel Babayana , Mbama Biloa Yves Julien , Bindi Josiane Gertrude Bwegne Ngasse , Temfemo Abdou , Mandengue Samuel Honoré , Guyot Jessica , Dupré Caroline , Barth Nathalie , Bongue Bienvenu , Etoundi Ngoa Laurent Serge , Ayina Ayina Clarisse Noel
Published: September 2023

Background

Living areas in developing countries impact seriously lifestyle by modifying energy consumption and energy expenditure. Thus, urbanization is associated with less practice of physical activity (PA), a leading cause of metabolic syndrome (MetS) which prevalence vary in African countries. The present study aimed to assess the effect of PA on MetS according to urbanization level in the littoral region, Cameroon.

Methods

A cross-sectional study was conducted in three geographical settings (urban, semi-urban, and rural) in the littoral region in Cameroon. A total of 879 participants were included (urban: 372, semi-urban: 195 and rural: 312). MetS was defined according to the International Federation of Diabetes 2009. The level of PA was assessed using the Global Physical Activity questionnaire.

Results

Low level of PA was (P < 0.0001) reported in urban (54.5%), semi-urban (28.7%) and rural (16.9%) and high level in rural area (77.9%). The prevalence of MetS was higher in urban areas (37.2%), then rural (36.8%) and finally semi-urban (25.9%). Hyperglycemia (p = 0.0110), low HDL-c (p < 0.0001) and high triglyceridemia (p = 0.0068) were most prevalent in urban residents. Participants with low level of PA were at risk of MetS (OR: 1.751, 95% CI 1.335–2.731, p = 0.001), hyperglycemia (OR: 1.909, 95% CI 1.335–2.731, p = 0.0004) abdominal obesity(OR: 2.007, 95% CI 1.389–2.900, p = 0.0002), low HDL-c (OR: 1.539, 95% CI 1.088–2.179, p = 0.014) and those with moderate level of PA were protected against high blood pressure(OR: 0.452, 95% CI 0.298–0.686, p = 0.0002) and compared to those with high level of PA. Urban dwellers were at the risk of MetS compared to rural residents (OR: 1.708, 95% CI. 1.277–2.285, p = 0.003) and protected against high blood pressure (OR:0.314, 95% CI 0.212–0.466, p < 0.0001), abdominal obesity (OR: 0.570, 95% CI 0.409–0.794, p = 0.0009), and low HDL-c (OR: 0.725, 95% CI 0.534–0.983, p = 0.038) compared to rural residents.

Conclusions

MetS was more prevalent in urban dwellers and was associated with a low level of PA.
Antimicrobial, antioxidant and anti-inflammatory activities of the root extracts and fractions of Terminalia avicennioides Guill. and Perr.
Authors: Aliyu-Amoo Hadiza , Isa Hamza Ibrahim
Published: September 2023

Background

Infectious diseases remain a major source of death and sickness globally. Their management entails use of antimicrobials. However, emergence of microbial resistance to these agents is on the increase. Inflammation and oxidative stress also accompany disease states. Newer agents to counter microbes, inflammation and oxidative stress are needed. Terminalia avicennioides Guill and Perr. is regularly exploited as a traditional remedy to numerous complaints, comprising infections, inflammation and enhancement of well-being. This study was intended to examine the effects of the root extracts and fractions of T. avicennioides against select bacteria and fungi as well as their anti-inflammatory and antioxidant potentials. Antimicrobial, anti-inflammatory and antioxidant assessments were conducted with broth microdilution technique, lipoxygenase and 2,2-diphenyl-1-picryhydrazyl (DPPH) assays, respectively.

Results

All the bacteria displayed varying susceptibility to the different extracts. The extracts caused good antibacterial activity towards Staphylococcus aureus, Salmonella typhimurium, Stenotrophomonas maltophilis, Enterobacter cloaca, Klebsiella pneumoniae, Echinococcus faecalis and Escherichia coli, but moderate for Proteus mirabilis and Pseudomonas aeruginosa. Antifungal action varied from good to moderate against Fusarium spp, moderate against Aspergillus niger but inactive against Candida albicans. Anti-inflammatory and antioxidant activities were dose-dependent, recording robust activity at higher concentrations. The n-butanol fraction manifested the highest anti-inflammatory and antioxidant activities, then ethylacetate while methanol extract showed better activities among hot and cold water extracts.

Conclusions

The analysis of the biological activities of T. avicennioides root extracts and fractions revealed encouraging antibacterial, antioxidant and anti-inflammatory activities, which were dose reliant. The different extracts and fractions displayed variable grades of activity. The plant showed good antibacterial but weak antifungal action. These activities could be credited to polyphenols and other plant constituents. This report may explain some of the traditional medicinal uses of the plant and could open the door for further studies in search of newer compounds against microbial organisms, inflammation and/or oxidative stress.
Hepatoprotective effects of ethnic cabbage dishes: a comparison study on kimchi and pao cai
Authors: Nugroho David , Surya Reggie , Nurkolis Fahrul , Surya Ervan , Thinthasit Aphinya , Kamal Nurkhalida , Oh Jin-Seok , Benchawattananon Rachadaporn
Published: September 2023

Consuming ethnic vegetable dishes, that has been an integral part in the food culture of many countries, is suggested to bring health benefits to humans. Kimchi from Korea and pao cai from China are two distinct vegetable dishes made from Chinese cabbage (Brassica rapa) through different processes. While kimchi is a fermented food, pao cai is produced by pickling in brine or vinegar. The present study aimed to investigate and compare the hepatoprotective effects of kimchi and pao cai in vivo using animal model and in vitro using a cell line. Despite having similar nutritional profiles, kimchi and pao cai exhibited different chemical and microbiological properties. Compared to pao cai, the pH during fermentation of kimchi dropped more rapidly and the antioxidant activity of kimchi was also stronger. In addition, total microorganisms and lactic acid bacteria were consistently higher in kimchi than in pao cai. In vivo, the hepatoprotective properties of kimchi and pao cai were associated with the increase in expression and activity of major liver antioxidant enzymes, particularly glutathione reductase, glutathione peroxidase, glutathione S-transferase, catalase, and superoxide dismutase. In vitro, both kimchi and pao cai promoted the formation of glutathione. Upon exposure to chemically induced oxidative stress, kimchi protected liver cells by inhibiting glutathione depletion and limiting lipid peroxidation. In general, kimchi demonstrated stronger hepatoprotective properties compared to pao cai. Thus, the present study provides promising insights into the development of ethnic foods, particularly kimchi and pao cai, as functional foods beneficial for human health.
Electrolyte profile in COVID-19 patients: insights into outcomes
Authors: Mohamed Mohamed Sorour , Negm Essamedin M. , Zahran Mahmoud Hosny , Magdy Mahmoud M. , Mohammed Ahmed Abdulsaboor , Ibrahim Dalia Anas , Tawfik Ahmed E. , Hassan Tarek Hamdy
Published: September 2023

Background

Some evidence has provided that electrolyte disorders may be present upon presentation of patients with COVID-19 infection. We investigated serum sodium, potassium, calcium, magnesium, and phosphorus levels in large numbers of critically ill COVID-19 patients to identify its possible prognostic value in these patients.

Methods

This retrospective study included patients confirmed with COVID-19 infection admitted to critical care units of Zagazig University Hospital all over 1 year, from May 1, 2020, to April 30, 2021. We analyzed the data for possible correlations between serum electrolytes and patients’ outcomes.

Results

Among 600 patients included in the study with a mean age of 51.33 ± 16.5 years, 44.16% were mechanically ventilated, and 30.66% died during hospital admission. Serum sodium, potassium, phosphorus, magnesium, and calcium were 141.96 ± 5.4, 4.33 ± 0.66, 3.76 ± 1.26, 2.21 ± 0.52, and 8.55 ± 0.85 respectively, at admission to the ICU. Unfavorable admission course and mortality were significantly associated with high normal serum sodium, potassium, and phosphorus levels and a low normal calcium level.

Conclusion

Although mean serum sodium, potassium, calcium, magnesium, and phosphorus were within normal levels in patients with COVID-19 at presentation, serum sodium, potassium, and phosphorus were significantly higher in those with poor outcomes, whereas calcium was significantly lower in those with poor outcomes.
Envisioning gamification in anesthesia, pain management, and critical care: basic principles, integration of artificial intelligence, and simulation strategies
Authors: Cascella Marco , Cascella Andrea , Monaco Federica , Shariff Mohammed Naveed
Published: September 2023

Unlike traditional video games developed solely for entertainment purposes, game-based learning employs intentionally crafted approaches that seamlessly merge entertainment and educational content, resulting in captivating and effective learning encounters. These pedagogical methods include serious video games and gamification. Serious games are video games utilized as tools for acquiring crucial (serious) knowledge and skills. On the other hand, gamification requires integrating gaming elements (game mechanics) such as points, leaderboards, missions, levels, rewards, and more, into a context that may not be associated with video gaming activities. They can be dynamically (game dynamics) combined developing various strategic approaches. Operatively, gamification adopts simulation elements and leverages the interactive nature of gaming to teach players specific skills, convey knowledge, or address real-world issues. External incentives stimulate internal motivation. Therefore, these techniques place the learners in the central role, allowing them to actively construct knowledge through firsthand experiences.

Anesthesia, pain medicine, and critical care demand a delicate interplay of technical competence and non-technical proficiencies. Gamification techniques can offer advantages to both domains. Game-based modalities provide a dynamic, interactive, and highly effective opportunity to learn, practice, and improve both technical and non-technical skills, enriching the overall proficiency of anesthesia professionals. These properties are crucial in a discipline where personal skills, human factors, and the influence of stressors significantly impact daily work activities. Furthermore, gamification can also be embraced for patient education to enhance comfort and compliance, particularly within pediatric settings (game-based distraction), and in pain medicine through stress management techniques. On these bases, the creation of effective gamification tools for anesthesiologists can present a formidable opportunity for users and developers.

This narrative review comprehensively examines the intricate aspects of gamification and its potentially transformative influence on the fields of anesthesiology. It delves into theoretical frameworks, potential advantages in education and training, integration with artificial intelligence systems and immersive techniques, and also addresses the challenges that could arise within these contexts.
How to quit cannabis when you have a mental illness: study from the perspective of patients who have successfully quit
Authors: Bruins Jojanneke , Crutzen Stijn , Veling Wim , Castelein Stynke
Published: September 2023

Aims and method

Research regarding quitting cannabis use often excludes patients with severe mental illness (SMI). We investigated facilitating and impeding factors in SMI patients and their advice to others, using semi-structured interviews with 12 SMI-patients, who were daily cannabis users for ≥12 months and had fully stopped using for ≥6 months.

Results

Seeking distraction, social contacts in personal environment, avoiding temptation and support from professionals were facilitating factors in stopping. Impeding factors were withdrawal symptoms, user environment, experiencing stress and user's routine. Advice to other patients included to just do it, seek support from others, quit ‘cold turkey’ and acknowledge that cannabis use is a problem. Advice to mental health professionals is to discuss cannabis use from the start of treatment.

Clinical implications

It is important to inform patients that cannabis use has negative consequences and limits the effects of treatment. Do not judge cannabis use or force the patient to stop.
Trastuzumab Deruxtecan in Patients With HER2-Mutant Metastatic Non–Small-Cell Lung Cancer: Primary Results From the Randomized, Phase II DESTINY-Lung02 Trial
Authors: Goto Koichi , Goto Yasushi , Kubo Toshio , Ninomiya Kiichiro , Kim Sang-We , Planchard David , Ahn Myung-Ju , Smit Egbert F. , de Langen Adrianus Johannes , Pérol Maurice , Pons-Tostivint Elvire , Novello Silvia , Hayashi Hidetoshi , Shimizu Junichi , Kim Dong-Wan , Kuo Chih-Hsi , Yang James Chih-Hsin , Pereira Kaline , Cheng Fu-Chih , Taguchi Ayumi , Cheng Yingkai , Feng Wenqin , Tsuchihashi Zenta , Jänne Pasi A.
Published: September 2023

PURPOSE

Trastuzumab deruxtecan (T-DXd) 5.4 and 6.4 mg/kg showed robust antitumor activity in multiple cancer indications; however, T-DXd 5.4 mg/kg has not been evaluated in patients with previously treated human epidermal growth factor receptor 2–mutant ( HER2m; defined as single-nucleotide variants and exon 20 insertions) metastatic non–small-cell lung cancer (mNSCLC).

METHODS

DESTINY-Lung02, a blinded, multicenter, phase II study, investigated T-DXd 5.4 mg/kg once every 3 weeks for the first time in previously treated (platinum-containing therapy) patients with HER2m mNSCLC and further assessed T-DXd 6.4 mg/kg once every 3 weeks in this population. The primary end point was confirmed objective response rate (ORR) per RECIST v1.1 by blinded independent central review.

RESULTS

One hundred fifty-two patients were randomly assigned 2:1 to T-DXd 5.4 or 6.4 mg/kg once every 3 weeks. As of December 23, 2022, the median duration of follow-up was 11.5 months (range, 1.1-20.6) with 5.4 mg/kg and 11.8 months (range, 0.6-21.0) with 6.4 mg/kg. Confirmed ORR was 49.0% (95% CI, 39.0 to 59.1) and 56.0% (95% CI, 41.3 to 70.0) and median duration of response was 16.8 months (95% CI, 6.4 to not estimable [NE]) and NE (95% CI, 8.3 to NE) with 5.4 and 6.4 mg/kg, respectively. Median treatment duration was 7.7 months (range, 0.7-20.8) with 5.4 mg/kg and 8.3 months (range, 0.7-20.3) with 6.4 mg/kg. Grade ≥ 3 drug-related treatment-emergent adverse events occurred in 39 of 101 (38.6%) and 29 of 50 (58.0%) patients with 5.4 and 6.4 mg/kg, respectively. 13 of 101 (12.9%) and 14 of 50 (28.0%) patients had adjudicated drug-related interstitial lung disease (2.0% grade ≥ 3 in each arm) with 5.4 and 6.4 mg/kg, respectively.

CONCLUSION

T-DXd demonstrated clinically meaningful responses at both doses. Safety profile was acceptable and generally manageable, favoring T-DXd 5.4 mg/kg.
Curcumin/Fusidic Acid Bitherapy Loaded Mixed Micellar Nanogel for Acne Vulgaris Treatment: In Vitro and In Vivo Studies
Authors: Abdel-monem Raghda , El-leithy Eman S. , Alaa-Eldin Ahmed Adel , Abdel-Rashid Rania S.
Published: September 2023

The combination of herbal drugs with a topical antibacterial for managing a chronic disease like acne vulgaris has emerged lately to settle side effects and bacterial multidrug resistance. Mixed micelles (MMs) incorporated into nanogel were explored for hybrid delivery of curcumin (Cur) and fusidic acid (FA) combination presenting a multi-strategic treatment. Curcumin-fusidic acid–loaded mixed micelles (Cur-FA-MMs) were assessed for size, surface charge, compatibility, in vitro release, and encapsulation. The selected formula was further loaded into nanogel and investigated for viscosity, ex vivo permeation, and in vivo potential. Cur-FA-MMs exhibited uniform nanosized spherical morphology, and negative surface charge affording high encapsulation for both drugs with a biphasic in vitro release over a period of 48h and good colloidal stability. The attained Cur-FA-MM-loaded nanogel had optimum viscosity with remarkable permeation coefficient values nearly 2-fold that related to plain nanogel. The pharmacodynamic effect of Cur on FA was pronounced by the significant improvement of the skin’s degree of inflammation, epidermal hypertrophy, and congestion in animals treated with Cur-FA-MM-loaded nanogel. In conclusion, micellar nanogel could enable the progressive effect of Cur (an antioxidant with reported antibiotic activity) on FA (antibiotic) and decrease the risk of emerging antibiotic resistance by enhancing the solubility and permeation of Cur.

Graphical Abstract
Study on noise enhancement injection in laser gyroscopes based on random noise variation rates
Authors: Bai Shicai , Ma Jiajun , Xu Yuxin , Gao Hui , Xu Wenzheng , Meng Jun
Published: September 2023

In the process of injecting random noise into a mechanical vibration laser gyroscope, the amplitude of the injected noise decays significantly as the noise frequency increases. To address this phenomenon, theoretical research was conducted on the transfer function of random noise in a mechanical vibration laser gyroscope, and a transfer function of random noise enhancement injection efficiency was established, which is related to the damping coefficient and resonant frequency. A method for enhancing random noise injection efficiency based on the rate of random noise level variation was proposed, and a circuit system for random noise enhancement injection in mechanical vibration laser gyroscopes was designed. The results showed that compared with the original random noise injection technology, random noise enhancement injection technology can effectively suppress the serious attenuation of high-frequency random noise amplitude, increase random noise injection efficiency by about 41.27%, reduce the random walk of the laser gyroscope's angle by about 17.73%, and improve its accuracy by about 27.02%. Random noise enhancement injection technology provides an important reference for improving the performance of mechanical vibration laser gyroscopes.
Influence of Vitamin D Deficiency on Cyclin D1-Induced Parathyroid Tumorigenesis
Authors: Costa-Guda Jessica , Corrado Kristin , Bellizzi Justin , Saria Elizabeth , Saucier Kirsten , Guemes-Aragon Miriam , Kakar Guntas , Rose Madison , Pascal Melanie , Alander Cynthia , Mallya Sanjay M , Arnold Andrew
Published: September 2023

Primary hyperparathyroidism (PHPT) is a common endocrinopathy for which several pathogenic mechanisms, including cyclin D1 overexpression, have been identified. Vitamin D nutritional status may influence parathyroid tumorigenesis, but evidence remains circumstantial. To assess the potential influence of vitamin D insufficiency/deficiency on initiation or progression of parathyroid tumorigenesis, we superimposed vitamin D insufficiency or deficiency on parathyroid tumor–prone parathyroid hormone–cyclin D1 transgenic mice. Mice were placed on diets containing either 2.75 IU/g, 0.25 IU/g, or 0.05 IU/g cholecalciferol, either prior to expected onset of PHPT or after onset of biochemical PHPT. When introduced early, superimposed vitamin D insufficiency/deficiency had no effect on serum calcium or on parathyroid gland growth. However, when introduced after the onset of biochemical PHPT, vitamin D deficiency led to larger parathyroid glands without differences in serum biochemical parameters. Our results suggest that low vitamin D status enhances proliferation of parathyroid cells whose growth is already being tumorigenically driven, in contrast to its apparent lack of direct proliferation-initiating action on normally growing parathyroid cells in this model. These results are consistent with the hypothesis that suboptimal vitamin D status may not increase incidence of de novo parathyroid tumorigenesis but may accelerate growth of a preexisting parathyroid tumor.
Black Geographies
Authors: Hawthorne Camilla , Lewis Jovan Scott
Published: September 2023

In the introduction, the editors present the history and implications of the Black Geographies discipline. In setting up the stakes for Black Geographies, the editors argue for the nonsingularity and nonuniversality of Blackness, its local and global reproduction through processes of circulation and diasporic routes, and the interplay of material and poetic processes. The introduction presents the epistemological challenges posed by Black Geographic thought to foundational categories such as space, scale, science, politics, and empire and how they draw attention to praxis to offer newer considerations of methodological and theoretical practice that emerges from the complex process of Black life.
Call Us Alive Someplace
Authors: Purifoy Danielle
Published: September 2023

Examining W. E. B. DuBois’s The Quest of the Silver Fleece and a collaborative study with Black abstract painter and conceptual artist Torkwase Dyson, both set in the Alabama Black Belt, this chapter traces Du Bois’s reckoning with positivist social science for alternative methods of Black study focused on affirming “Black livingness.” The chapter argues for a Du Boisian methodology in pursuing Black geographies—attention to the political economic and the poetic in rejecting social death as the primary means of characterizing Black life and place-making. The reason for this is about creating an alternative to measuring Black life against white life, rather than Black life on its own terms.
Detection Rates and Trends of Asymptomatic Unruptured Intracranial Aneurysms From 2005 to 2019
Authors: Laukka Dan , Kivelev Juri , Rahi Melissa , Vahlberg Tero , Paturi Jooa , Rinne Jaakko , Hirvonen Jussi
Published: September 2023

BACKGROUND AND OBJECTIVES:

The trend in detection rates of asymptomatic unruptured intracranial aneurysms (UIAs) on brain computed tomography angiography/magnetic resonance angiography (CTA/MRA) is not well established. Our objective was to evaluate time trends in asymptomatic UIA detection rates on brain CTA/MRA between 2005 and 2019.

METHODS:

We conducted a retrospective study of all brain computed tomography/magnetic resonance scans (n = 288 336 scans in 130 621 patients) performed between January 2005 and December 2019 at a tertiary referral hospital. Patients who underwent brain CTA/MRA examinations were included (n = 81 261 scans in 48 037 patients). The annual detection rate of new UIA cases was calculated based on the first brain CTA/MRA imaging. Detection rates were compared between three periods and across different age groups.

RESULTS:

The number of first CTA/MRA examinations increased significantly from 2005 to 2009 (n = 12 190 patients) to 2010–2014 (n = 14 969 patients) and 2015–2019 (n = 20 878 patients) (P < .001). The UIA detection rate also increased significantly from 1.7% in 2005–2009 to 2.5% in 2010–2014 and 3.4% in 2015–2019 (P < .001). The UIA detection rate increased significantly from 2010–2014 to 2015–2019 (relative risk [RR], 1.33; 95% CI, 1.17-1.51), particularly in patients aged 60–69 years (RR, 1.29; 95% CI, 1.01-1.63), 70–79 years (RR, 1.71; 95% CI, 1.30-2.25), and >79 years (RR, 2.33; 95% CI, 1.56-3.47). Furthermore, the detection rate of <5-mm UIAs increased from 2010–2014 to 2015–2019 (RR, 1.51; 95% CI, 1.28-1.77).

CONCLUSION:

The detection rate of asymptomatic UIAs, particularly in elderly patients, has increased significantly over the past 15 years, coinciding with the increased use of CTA/MRA imaging. Furthermore, the size of the identified UIAs has decreased. These findings raise concerns about the management strategies for UIAs, indicating the need for further research.
Neuronal Encoding of Speech Features in the Human Thalamus in Parkinson's Disease and Essential Tremor Patients
Authors: Tankus Ariel , Lustig-Barzelay Yael , Gurevitch Guy , Faust-Socher Achinoam , Strauss Ido
Published: September 2023

BACKGROUND AND OBJECTIVES:

The human thalamus is known, from stimulation studies and functional imaging, to participate in high-level language tasks. The goal of this study is to find whether and how speech features, in particular, vowel phonemes, are encoded in the neuronal activity of the thalamus, and specifically of the left ventralis intermediate nucleus (Vim), during speech production, perception, and imagery.

METHODS:

In this cross-sectional study, we intraoperatively recorded single neuron activity in the left Vim of eight neurosurgical patients with Parkinson's disease (PD) (n = 4) or essential tremor (n = 4) undergoing implantation of deep brain stimulation (n = 3) or radiofrequency lesioning (n = 5) while patients articulated the five monophthongal vowel sounds.

RESULTS:

In this article, we report that single neurons in the left Vim encode individual vowel phonemes mainly during speech production but also during perception and imagery. They mainly use one of two encoding schemes: broad or sharp tuning, with a similar percentage of units each. Sinusoidal tuning has been demonstrated in almost half of the broadly tuned units. Patients with PD had a lower percentage of speech-related units in each aspect of speech (production, perception, and imagery), a significantly lower percentage of broadly tuned units, and significantly lower median firing rates during speech production and perception, but significantly higher rates during imagery, than patients with essential tremor.

CONCLUSION:

The results suggest that the left Vim uses mixed encoding schemes for speech features. Our findings explain, at the single neuron level, why deep brain stimulation and radiofrequency lesioning of the left Vim are likely to cause speech side effects. Moreover, they may indicate that speech-related units in the left Vim of patients with PD may be degraded even in the subclinical phase.
Chondroitin sulfate is required for follicle epithelial integrity and organ shape maintenance in Drosophila
Authors: Knudsen Collin , Woo Seuk Koh  , Izumikawa Tomomi , Nakato Eriko , Akiyama Takuya , Kinoshita-Toyoda Akiko , Haugstad Greg , Yu Guichuan , Toyoda Hidenao , Nakato Hiroshi
Published: September 2023

Heparan sulfate (HS) and chondroitin sulfate (CS) are evolutionarily conserved glycosaminoglycans that are found in most animal species, including the genetically tractable model organism Drosophila. In contrast to extensive in vivo studies elucidating co-receptor functions of Drosophila HS proteoglycans (PGs), only a limited number of studies have been conducted for those of CSPGs. To investigate the global function of CS in development, we generated mutants for Chondroitin sulfate synthase (Chsy), which encodes the Drosophila homolog of mammalian chondroitin synthase 1, a crucial CS biosynthetic enzyme. Our characterizations of the Chsy mutants indicated that a fraction survive to adult stage, which allowed us to analyze the morphology of the adult organs. In the ovary, Chsy mutants exhibited altered stiffness of the basement membrane and muscle dysfunction, leading to a gradual degradation of the gross organ structure as mutant animals aged. Our observations show that normal CS function is required for the maintenance of the structural integrity of the ECM and gross organ architecture.
Modeling a variant of unknown significance in the Drosophila ortholog of the human cardiogenic gene NKX2.5
Authors: Lovato TyAnna L. , Blotz Brenna , Bileckyj Cayleen , Johnston Christopher A. , Cripps Richard M.
Published: September 2023

Sequencing of human genome samples has unearthed genetic variants for which functional testing is necessary to validate their clinical significance. We used the Drosophila system to analyze a variant of unknown significance in the human congenital heart disease gene NKX2.5 (also known as NKX2-5). We generated an R321N allele of the NKX2.5 ortholog tinman (tin) to model a human K158N variant and tested its function in vitro and in vivo. The R321N Tin isoform bound poorly to DNA in vitro and was deficient in activating a Tin-dependent enhancer in tissue culture. Mutant Tin also showed a significantly reduced interaction with a Drosophila T-box cardiac factor named Dorsocross1. We generated a tinR321N allele using CRISPR/Cas9, for which homozygotes were viable and had normal heart specification, but showed defects in the differentiation of the adult heart that were exacerbated by further loss of tin function. We propose that the human K158N variant is pathogenic through causing a deficiency in DNA binding and a reduced ability to interact with a cardiac co-factor, and that cardiac defects might arise later in development or adult life.