bluuebunny/crossref_metadata_2025 huggingface dataset:
Varidnaviruses in the human gut: a major expansion of the order Vinavirales
Authors: Yutin Natalya , Rayko Mike , Antipov Dmitry , Mutz Pascal , Wolf Yuri I , Krupovic Mart , Koonin Eugene V
Published: July 2022

Background
Bacteriophages play key roles in the dynamics of the human microbiome. By far the most abundant components of the human gut virome are tailed bacteriophages of the realm Duplodnaviria, in particular, crAss-like phages. However, apart from duplodnaviruses, the gut virome has not been dissected in detail.
Results
Here we report a comprehensive census of a minor component of the gut virome, the tailless bacteriophages of the realm Varidnaviria. Tailless phages are primarily represented in the gut by prophages of the families Corticoviridae and Autolykiviridae that jointly comprise the order Vinavirales and are mostly integrated as prophages in genomes of Alphaproteobacteria and Verrucomicrobia. Phylogenetic analysis of the major capsid proteins (MCP) and packaging ATPases suggests that at least three new families within Vinavirales should be established to accommodate the diversity of prophages from the human gut virome. Previously, only the MCP and ATPase genes were reported to be conserved in all members of Vinavirales. Here we identify a core set of 12 proteins that are shared by most of these viruses including previously undetected lysis enzymes. We further demonstrate that replication system components are frequently replaced in the genomes of Vinavirales, suggestive of selective pressure for escape from yet unknown host defenses or avoidance of incompatibility with coinfecting related viruses.
Conclusions
The results of this analysis show that, in a sharp contrast to marine viromes, varidnaviruses are a minor component of the human gut virome. Moreover, they are primarily represented by proviruses, suggesting that there are few if any active varidnavirus infections in the gut at any given time. These findings complement the existing knowledge of the human gut virome by exploring a group of viruses that was virtually overlooked in previous work.
Anticancer and Apoptotic Activity in Neuroblastoma SK-N-SH using Phospholipid Extract from Bone of Scomberomorus niphonius
Authors: Kiddane Anley Teferra , Roy Vikash Chandra , Kang Min-Jae , Patil Maheshkumar Prakash , Chun Byung-Soo , Kim Gun-Do
Published: July 2022

Among various types children’s health challenges, Neuroblastoma is the most serious solid neoplasm forming outside the cranium. S. niphonius is a valuable edible fish that has been widely used for a meal. In this study, we obtained phospholipid extract from the bone of S. niphonius with the supercritical CO2 extraction method and tested anticancer activity with a cell viability assay. The phospholipid showed anticancer activity on neuroblastoma SK-N-SH cells, and the anticancer activity was presented with an IC50 of 710.25 ± 28.31 µg/ml, but didn’t show a significant toxicity on HUVEC cell lines. Westernblot was used to detect signaling proteins; Bak, caspase-9, caspase-8, caspase-3, Bax and IκBα were increased, whereas IKKβ and NFκB were downregulated in experimental groups compared to untreated groups. Gene expression was revealed by RT-qPCR, and the fold ratio of Apaf-1, cytochrome-c, caspase-9, caspase-3 and Bax genes’ expression was raised in treated groups, implying apoptosis. Gel electrophoresis revealed that the experimental groups had more fragmented DNA than the control group. The study shows that a phospholipid extract from S. niphonius’s bone could be used as a biological origin of anticancer activity in neuroblastoma SK-N-SH cells.
Machine learning and fuzzy logic application in predicting the effect of dynamic loading on rectangular armed panels - a comparison on impact loading
Authors: Tafarroj M. M. , Hatami H.
Published: July 2022

In a comprehensive experimental work, using of four basic mix designs, 64 rectangular composite panels were made with 100mm2 of area and 30, 45, 60, and 75mm thickness and tested by impact loading. Compressive, tensile, and flexural strength tests were performed on all the four max designs. Steel fibers with percentages of 0, 0.25, 0.5, and 0.75 with 25m of length were used to make the concrete composites. A hammer with 180kg weight and 7500J power was used as the impact loading with drop hammer test machine (DH-TM). The specimens were dynamically loaded by drop test from a 60cm height. The using of steels fibers and expanded sheets in combination with each other significantly increase the energy absorption. Moreover, the initial peak force increases, while crushing length and deformation of the specimens reduce. In the remainder of the study, using the data obtained from experiments, models based on two well-known techniques-namely artificial neural networks (ANN) and adaptive network-based fuzzy inference system (ANFIS) have been developed to construct a model for the problem. The performance of both techniques is compared and finally the most appropriate one for predicting new data is introduced.
Estimating the impacts of environmental management on strawberry yield using publicly available agricultural data in South Korea
Authors: Kim Steven , Jo Jung Su , Luk Vicky , Kim Sung Kyeom , Kim Dong Sub
Published: July 2022

Advanced information and communication technologies have made data collection more efficient, and crop growth, yield, and environmental data of multiple farms can be used for various purposes. Using the publicly available database in South Korea, we investigated the correlation between the management of greenhouse environments and the pattern of strawberry yield during harvest seasons. Location-specific datasets were merged and processed, and mixed-effects models were applied to account for both observable greenhouse conditions and unobserved factors associated with farm locations. Instead of using averaged temperature and relative humidity inside greenhouses, we estimated the percent of time that air temperature has been between 15°C and 20°C, denoted by T%, and the percent of time that relative humidity has been between 0% and 50%, denoted by H%. The strawberry yield tends to reduce with respect to time during a harvest season, but the reduction rate is slower when the T% and H% are higher. This study provides empirical evidence for the practical significance of maintaining air temperature and relative humidity within an optimal range, and we conclude that growers can mitigate loss of production by the maintenance of air temperature and relatively humidity particularly during the later phase of a harvest season.
Molecular Detection of Spotted Fever Group Rickettsiae and Ehrlichia in Rodents from Anhui Province, China
Authors: ADAMS ABDUL LATIF , BO-YU Liu , ZHEN CHEN , OBIRI-YEBOAH DORCAS , LIU YAN
Published: July 2022

BackgroundRickettsioses are important zoonotic diseases worldwide. Rickettsia are naturally maintained in vertebrate hosts and can emerge or reemerge with serious public health implications. Rodents are important host animals for many rickettsial pathogens. In China, several species of rickettsia have been reported in tick species, and few have been reported in vertebrate host animals, including rodents. Epidemiological studies that investigate natural foci and the role of small mammals in the transmission of zoonoses to humans are still needed. Thus, we investigated the presence of rickettsial agents in rodents from Anhui Province, China.MethodsA total of 143 rodents, including 131 Apodemus agrarius and 12 Niviventer niviventer, were captured alive using cage traps in the fields of four areas in Anhui Province. Tissue samples were collected aseptically from each rodent. DNA samples from rodent tissues were amplified by PCR based on rickettsial genes: the 17-kilodalton antigen gene (17-kDa), 16S rRNA gene (rrs) and citrate synthase gene (gltA). Positive amplicons were sequenced and confirmed by BLAST analysis. Phylogenetic analysis was performed by Mega 7.0 software.ResultsThe overall prevalence rate of rickettsial agents in rodents was 11.9% (17/143). The genes encoding the common rickettsial 17-kDa, gltA, and 16S rRNA were positively detected in 2.1% (3/143), 5.6% (8/143) and 4.2% (6/143) of the rodents, respectively, by nested PCR. The rickettsial agents detected included Rickettsia raoultii, Rickettsia japonica and Ehrlichia chaffeensis according to BLAST and phylogenetic analysis.ConclusionThis study demonstrates the presence of rickettsial agents in rodents captured in Anhui Province, China. Further epidemiological studies should be investigated in arthropod vectors, host animals and humans to estimate the impact of rickettsia infestations among rodents from these areas.
Supine Paediatric Percutaneous Nephrolithotomy: single centre experience with selective tubeless approach
Authors: Taghavi Kiarash , Pirpiris Athina , Webb Nathalie , Coleman Robert , McCahy Philip
Published: July 2022

Objective: Advances in percutaneous nephrolithotomy (PCNL) toward less invasive treatment options and miniaturisation of equipment have particular application in paediatric patients. The current study aims to establish the safety and efficacy of the modified-supine position in children and a selective “tubeless” approach to paediatric PCNL.Methods: A retrospective cohort study was performed of all paediatric patients undergoing PCNL at our tertiary institution over 10 years (2010-2019).Results: Of 635 PCNL patients, 20 were paediatric (3%). Staghorn calculi (complete or partial) made up 35% of the population. The majority of procedures were performed in the modified-supine position (85%). Median procedure time was 2.1 hours, and length of stay was 2.2 days. With regards post-operative drainage, a ureteric stent was not placed in 75% of cases. The stone free rate was 65%. The median follow-up time was 8.7 years (IQR 6.7-9.9 years).Conclusion: As a result of technical and technological advances, PCNL can be considered a safe and effective procedure in children with a large stone burden, in situations of anatomic complexity or when immediate stone-free status is required. Selectively reducing post-operative drainage and utilising a modified supine position is safe, efficacious and may provide additional benefits in children.
Materialistic happiness menaces people’s meaning in life: A serial double mediation model reflecting the roles of basic psychological needs satisfaction and subjective well-being
Authors: Lo On-Ting , Cheung Sing-Hang , Lai Veronica Ka Wai
Published: July 2022

Background: People are holding stronger materialistic belief than before, especially in some developed regions in the world. Materialism refers to beliefs that equate materialistic possessions with happiness, success, and make it central to life. Extant research has widely shown that materialism threatens people’s well-being. Nevertheless, the evidence on how materialism influences meaning in life is less well-established. Within the realm of this area, we examined the relationship between materialism and meaning in life, as well as its potential mechanisms, under the framework of Self-Determination Theory.Methods: Two cross-sectional online survey studies have been conducted to test the hypothesized serial double mediation model. In this model, we measured four constructs including people’s materialistic values, basic psychological needs satisfaction, subjective well-being, and meaning in life. In Study 1, we collected data from a Chinese sample (N=190). In Study 2, we tried to replicate the findings from Study 1 by collecting a non-Chinese different-age Prolific sample (N=767).Results: The results from Study 1 and Study 2 are consistent. We found that the relationship between materialistic happiness and meaning in life is serially mediated by basic psychological needs satisfaction and subjective well-being. The results confirm that materialistic happiness frustrates basic psychological needs, which threatens people’s subjective well-being then meaning in life. Nonetheless, same serial multiple mediation model is not observed from other two materialistic dimensions including materialistic centrality and materialistic success.Conclusions: Materialistic happiness threatens people’s sense of meaning in life. This relationship can be explained under the framework of Self-Determination Theory. Issues such as how different types of happiness facilitate the constitution of meaning in life, and new investigation directions on materialism and meaning in life research have been discussed.
New Magellanic penguin Spheniscus magellanicus colony in a subantarctic island: good or bad news?
Authors: Rey Andrea Raya , Balza Ulises , Domato Ignacio , Zunino Francisco
Published: July 2022

This paper provides data on a newly discovered colony for Magellanic penguin Spheniscus magellanicus in the southwest Atlantic Ocean. A new settlement for the species in the area was found while conducting southern rockhopper penguin Eudyptes chrysocome studies in the San Juan de Salvamento colony in Staten Island, Argentina. The newly established colony holds 88 breeding pairs. While the origin of the founders was not recognized, it is possible that individuals recently reached from the nearby Observatorio Island colony located ~ 20 km away. New colonies grant populations to expand and colonize new areas which holds promise in a climate change scenario. Nonetheless, this expansion might be in detriment of sibling species that already occupied the area.
Co-occurrence of health risk behaviors and associated factors among adolescent students: Findings from a cross-sectional study in Bangladesh
Authors: Rahman Md. Estiar
Published: July 2022

Aim: This study aimed to determine the prevalence and associated factors of co-occurring health risk behaviors among Bangladeshi adolescents.Subject and Methods: A cross-sectional study involving 637 adolescent students in three high schools and three colleges in Dhaka city, Bangladesh, was conducted from November 2019-February 2020. Participants were selected using a multistage sampling technique. A self-administered questionnaire was used to collect data and statistical package for social sciences, version 22.0 was used to perform data analysis.Results: The participants comprised of 57.1% male, and their mean age was 15.65 ± 1.58 years. Among the participants, 2.4 % (n=15) reported one health risk behavior, while 0.3 % (n = 2) reported no risk behaviors. The prevalence of co-occurring health risk behaviors among the participants was 26.4%. Logistic regression analysis showed that co-occurrence of health risk behaviors was associated with participants aged with 17-18 years (OR = 3.11; 95% CI = 1.95-4.95, p < .001), college students (OR = 2.74, 95% CI = 1.90-3.94, p < .001), participants having secondary level of fathers’ education (OR=2.63; 95% CI=1.68-4.09, p < .001), participants having secondary level of mothers’ education (OR = 1.84; 95% CI = 1.12-3.02, p = .015), living apart form family (OR = 2.22; 95% CI = 1.37-3.59, p = .001), poor academic performance (OR=2.06; 95% CI = 1.26-3.36, p = .004), and reporting truancy (OR = 1.81; 95% CI = 1.20-2.72, p = .005).Conclusion: The findings emphasize the need for effective interventions to prevent risk behavior practices among adolescents.
Using LEXSY plasmids for sodb1 gene silencing in Leishmania tropica parasites
Authors: Alhraki Fatema , Kassouha Morshed , Soukkarieh Chadi
Published: July 2022

Background: Genetic modifications and manipulations have topped the list of recent research, as they target the causes, not the results, and are currently considered one of the most powerful methodologies used to study the biology of the Leishmania parasites. These methods have expanded since the publication of the first study in which the genetic replacement of one of the Leishmania genes was done, which provided an opportunity to analyze and study the biology of the parasite genomically. In this study, the SODB1 gene was targeted by antisense RNA. This gene encodes one of the important enzymes in the infectivity of Leishmania tropica parasites within macrophages, which is the superoxide dismutase enzyme (SODB1).Methods: An inverted sequence of part of SODB1 ORF and 3’UTR were cloned in LEXSY plasmid, and after obtaining the silencing constructions, the Leishmania tropica parasites were transfected by electroporation. Western Blot analysis of SODB1 expression and the infectivity of these mutant parasites in human macrophages was studied and evaluated in comparison with a wild-type negative control and another control containing the GFP gene that codes for Green Fluorescent Protein (GFP).Results: The results showed that using LEXSY plasmids for SODB1 gene silencing was efficient and the knocking-down was clear forasmuch the decrease in both infectivity and parasite load in human macrophages in vitro. On the other hand, western blot analysis revealed a lower expression level of SODB1 in the mutant parasites than wild-type. The results were subjected to statistical analysis and the decreases in the infectivity and parasites load of the mutant parasites were very significant in comparison with the wild-type.Conclusion: Our study confirms the efficiency of the produced silencing system, and the importance of the SODB1 enzyme in the amastigote parasites’ ability to grow and survive within the host's macrophages despite the presence of many other SODs enzymes. This is the first study that affirms the success of using the LEXSY gene expression system for gene silencing according to the antisense RNA approach. Thus, this work will allow using of the produced silencing system to target other important genes in Leishmania, and continue studying the knocked-down strain in vivo.
Body surface area as a prognostic predictor in patients with sepsis
Authors: Chang Haihua , Liao Lin , Wang Wei , Pinhu Liao
Published: July 2022

Background
Sepsis is a common inflammatory syndrome, and its mortality is associated with obesity. Body surface area (BSA) and body mass index (BMI) are closely related to body weight, height but BSA is more closely related to physiological parameters. The relationship between BSA and sepsis mortality has not been well known. This study aimed to assess the association between BSA and 28-day mortality among patients with sepsis hospitalized in the intensive care unit.
Methods
Information of sepsis patients were collected from the Medical Information Market in Intensive Care (MIMIC)-III database. BSA was calculated by using the Mosteller formula and grouped by gender and BSA tertiles. Kaplan-Meier curves and Cox proportional hazards analyses were the main statistical methods used. Restricted cubic splines were utilized to visualize the associations between continuous BSA and 28-day all-cause mortality.
Results
Sepsis patients (N = 2507) involved in the study included 1491 males (59.47%) and 1,016 females (40.53%). A linear negative association was found between the BSA and 28-day all-cause mortality in male patients. A higher BSA was independently associated with a lower death risk [hazard ratio (HR) 0.487, 95% confidence intervals (CI) 0.271–0.873; P = 0.016], but no association was found in females (HR 0.559, 95% CI 0.289–1.082; P = 0.840). Cox proportional hazards analysis showed that the BSA was an independent protective factor for 28-day outcomes in males with sepsis.
Conclusions
A linear inverse association was observed between the BSA and 28-day mortality risk in male sepsis patients.
Higher efficiency of CRISPR/Cas9-mediated knock-in in HSV-2 by cleaving donor plasmid and viral target region simultaneously
Authors: Qiu Guifang
Published: July 2022

The ability of engineering herpes simplex virus (HSV) underpins much in the reseach of its pathogeny biology and oncolytic virus therapy. Currently, the CRISPR/Cas9 system serves as the most powerful tool of HSV genome editing in human cells undoubtedly. Here, comparing the efficiency of gene insertion into HSV-2 based on CRISPR/Cas9-induced asymmetry and symmetry double-sided homology arms donor mediated knock-in strategies, we found that the former is better. After co-transfection of Cas9-sgRNA co-expression plasmid and a donor plasmid containing foreign gene flanked by double-sided homology arms of viral genome target site into HEK293T cells followed by infection of HSV-2, concurrent cleavage of unilateral homology arm within donor plasmid and viral selected site resulted in higher efficiency of targeted gene integration. EGFP cassette was successfully inserted into non-coding region of HSV-2 employing this approach. This would be a more efficient gene insertion strategy via increasing the frequency of HDR and saving time in construction of donor template, thus facilitating the study progress to a great extent.
Incidence and Risk Factors of Hypophosphatemia in Patients with HIV Infection Receiving Tenofovir Disoproxil Fumarate at a Hospital Belonging to a Medical Service Department
Authors: Netsuk Praewa , Jullabath Jirapat , Lakum Pornchita , Phuetthanyakij Passara , Phuawaranukhroh Pongsri , KarunratTewthanom
Published: July 2022

Recently, the number of patients with human immunodeficiency virus (HIV) infection in Thailand tends to decrease because of more access to treatment, including the discovery and development of a combination of highly effective antiretroviral drug containing tenofovir disoproxil fumarate (TDF) and its widespread use. This is also attributed to their good efficacy and few adverse reactions. However, the nephrotoxic effect of TDF has been reported, including cases of low blood phosphate. To our knowledge, no study has investigated the incidence of hypophosphatemia caused by TDF in Thailand. This retrospective cross-sectional study aimed to estimate the incidence and risk factors of hypophosphatemia in patients receiving TDF-containing anti-HIV regimens. Data of patients with HIV infection who received TDF between July 1, 2018, and June 30, 2020, at a hospital belonging to a medical service department were reviewed. Data such as serum creatinine and serum phosphate levels were collected from medical records and electronic medical records and then transferred through a data record form. Hypophosphatemia was defined as serum phosphate value lower than 2.5 mg/dL. As a result, from 798 cases of patients with HIV infection who received TDF, 26 patients met the inclusion criteria and five patients had hypophosphatemia (19.2%), and the standard drug dose was used (300 mg/day) or was properly adjusted according to patients’ renal function. The median duration of TDF use was 10 (1–63) months. Other factors that may contribute to the development of hypophosphatemia are comorbidities and other drugs; there was one patient who used antacids longer than 1 week before onset of hypophosphatemia. This study may help develop a risk assessment tool for monitoring hypophosphatemia in patients who received TDF.
Gender and sexuality in contemporary Malaysian popular fiction in English (2010-2020) by Malay Muslim authors
Authors:   , Hamidi Faraha
Published: July 2022

This study explores literary representations of gender and sexuality in contemporary Malaysian Popular Fiction in English (MPFE) written by Malay Muslim authors that are published in between the years 2010 to 2020. It questions why gender and sexuality are considered sensitive topics and the public discussion of these topics is deemed taboo by some Malay Muslim traditionalists and contemporary scholars of Malay literature. Previous studies suggests that Islamic rules and regulations influence the Malaysian Malays worldview. Its sacred book, the Quran, has established clear-cut prohibitions against any sexual indulgence among its believers. Muslim writers must learn to restrict themselves from indulging in sexual writings in order to prevent them from intentionally or unintentionally arousing their readers’ sexual fantasies that may lead both parties to sinning. However, at the end of the twentieth century, many factors such as the impact of modernisation through scientific and industrial revolution on Malaysian society, the influence of Western Humanities theories among local intellects, and the introduction of Internet culture have contributed tremendously to the dramatic social changes in Malaysia. These changes are reflected heavily in its literary culture. In recent years, the Malay people’s awareness of their body and individuality is heightened. There is a surge of curiosity among contemporary Malay Muslims about their gender and sexuality and they would want a discussion. Following this development, the first objective of this study is to provide the latest discussion on gender and sexuality in MPFE by Malay Muslim authors. The second objective is to provide observations on how MPFE authors employ their literary strategies to approach aspects of gender and sexuality in their literary works. It pays attention to how writers express their acceptance, negotiations, and/or rejections towards the dominant “normative” or “common” values in the Malay society with regards to their body and sexuality. Using textual analysis to examine one novel and six short stories from the MPFE genre, this paper cross-examines Malay literary theories on sexual and erotic literature available in Pengkaedahan Melayu (Malay Methodology), Persuratan Baru (Genuine Literature), as well as Western theoretical approaches in Postcolonialism, Postmodernism and Feminism on gendering system and sexuality, in its aim to explain the growing interest in the topics in spite of the red-tape around sexual taboos in Malaysian literature.
CHRONICLE CITY OF KHOTEN
Authors: OSADCHYI YEVHEN M. , DEGTYAREV SERHII I.
Published: July 2022

The article discusses issues related to the localization of the chronicle city
of Khoten and the eponymous volost of the Putivl district in the second half of the 13th –
16th centuries. To resolve this issue, a number of written and archaeological sources are
used to determine the approximate boundaries of the Khoten volost. For the first time the name of the city of Khoten is found in the chronicles in the XIV century. This settlement is mentioned among the cities located on the Sula River. Later, Khoten was mentioned in the documents of the Crimean Khanate and the Grand Duchy of Lithuania. Unfortunately, these documents do not give an exact answer where exactly Khoten was located. Since the end of the 19th century, historians have localized the chronicle city of Khoten on the territory of the village of the same name in the Sumy region. However, archaeological prospecting did not reveal the remains of an ancient settlement there. In the documents of the 16th century, there is a clarification of the location of the Khoten volost, which was located between the rivers Psel and Khorol. Analysis of the fortified hilforts of this region, outlined by the boundaries of the volost, makes it possible to identify some that meet the criteria of the ancient Russian annalistic city. This is a hillforts in the village of Knyshovka and the town of Gadyach, Poltava region. They have an area of about 1.5 hectares. Knyshovka hillfort has a dating corresponding to the chronicle data and confirmed by archaeological research. The hillfort in the city of Gadyach was built before 1626, but it has no dating according to the data of archaeological research. An analysis of the location and features of these two hillforts suggests that the remains of the ancient Russian chronicle city of Khoten may be a hillfort in the city of Gadyach.
Activities of the state labour reserves system as an urbanization factor in after-war Ukraine (1945 – the early 1950s).
Authors: KOROL VITALIY  M.
Published: July 2022

The article is devoted to analyzing the role, scale of influence and significance of the Labour Reserves system for the post-war urbanization processes in Ukraine.
The State Labour Reserves (SLR) was a Soviet centralized structure that mobilized young people, trained skilled workers, and accumulated and distributed this new personnel among enterprises in strategic industries and infrastructure. The activity of the State Labour Reserves system of the USSR became a rather specific factor that played a significant role in the urban development of Ukraine during the period of Late Stalinism.
The real impact of the Labour Reserves on urbanization processes was:
– the mechanical movement of a significant number of young people from rural areas to cities;
– the transformation of potential workers in the agricultural sector into industrial workers, increasing the share of the working class compared to the collective and state farm peasantry;
– the creating conditions for inculcating the urban way of life to young people mobilized from the countryside, their incorporation into urban society.
The real reasons for the establishment of forced conscription into SLR facilities were the general militarization of the economy and society at the time, as well as the unpopularity of the hard-working jobs to which conscripts were sent, effectively using non-economic coercion.
It was assumed that a significant mass of young people had to move centrally from rural areas to industrial centres (that is, urban settlements).
Youth appeals to SLR facilities during the post-war reconstruction led to the organized migration of about three-quarters of a million young people from rural areas to cities. The cities of Eastern Ukraine (mostly Donbas) were the most significant areas of such centralized movement under the auspices of the Labour Reserves.
Soviet labour laws of the 1940s were analyzed. It was found that fleeing Labour Reserves and illegally leaving the workplace was considered a criminal offence and punishable by imprisonment.
The USSR State Labour Reserves system became one of the tools to control and regulate urbanization processes in the country for the Stalinist totalitarian regime.
Mobilized boys and girls were involved in non-agricultural sectors of the economy. They were forced to quickly get used to anonymous transactions among the large population of Ukrainian cities and inevitably changed their way of life, becoming part of a new generation of urban society.
The research is based on materials from the Central State Archives of Supreme Bodies of Power and Government of Ukraine and the State Archives of Sumy region.
Methodology for the identification of bioactive and marker peptides in the organs of cattle and pigs
Authors: Khvostov D. V. , Vostrikova N. L. , Chernukha I. M.
Published: July 2022

The development of general conception methodology for the meat-based functional food compositions is especially relevant today due to the growing consumers’ interest and attention to their health. This category of these food-products is intended for personal  ized nutrition of various age groups in the population, taking into account fortification of the food with nutraceuticals and with functional and metabolically active ingredients obtained from animal and vegetable source. Therefore, it was necessary to develop a certain tool for reliable identification of free peptides from the offals (by-products like hearts and aorta from Sus scrofa and Bos taurus) and from the ready-to-consume meat food (canned food) based on the free peptides, which food is potentially targeted to help with some issues in the human body. The authors proposed the methodology for identification of peptides weighing less than 5 kDa. This methodology has a row of significant advantages, such as a short time of analysis (90 minutes) and the possibility to prepare a large number of samples simultaneously (n=16). Analysis of bioactive peptides (BAPs) was performed by liquid chro  matography combined with time-of-flight mass spectrometry (Agilent 6545XT AdvanceBio LC/Q-TOF). The marker peptides were detected by a triple quadrupole mass spectrometer (Agilent 6410 Triple Quadrupole LC/MS). All peptide sequences were defined with the help of mass spectrometric data processing databases like PepBank, BioPep, AHTPDB. In this work from 39 to 269 peculiar soluble peptides were found, with an extraction level of 0.17–0.23%. The main fraction consisted of short peptides less than 1000 Da (71.0–98.0%). In experimental samples of pork hearts and arteries 7 peculiar marker peptides were identified. FFESFGDL  SNADAVMGNPK peptide obtained from the β-hemoglobin protein is of a special interest, as this peptide showed the maximum intensity of a signal. Presumably, this peptide can serve as an indicator of the blood presence in the finished food product. So it can serve as an assessment tool of bleeding degree of meat raw. For pork aortas a specific peptide TVLGNFAAFVQK was isolated from serum albumin, which turned out to be stable during heat treatment. This is also important for assessment of meat food that are subjected to high thermal exposure.
Loop-mediated isothermal amplification (LAMP) method for fast detection of Campylobacter spp in meat food products and environmental objects of a processing plant’s
Authors: Yushina Yu. K. , Zajko E. V. , Grudistova M. A. , Reshchikov M. D. , Nasyrov N. A. , Nikitchenko D. V.
Published: July 2022

There is constant necessity of developing the accurate and fast methods for detection of foodborne pathogens. Microorganisms of Campylobacter genus are one of the main causes of foodborne diseases worldwide. Fast identification of Campylobacter at all stages of the food life cycle is an efficient strategy to control foodborne campylobacteriosis. This article the authors evaluated a commercial loop-mediated isothermal amplification (LAMP) system with bioluminescence, called as the 3M™ Molecular Detection Analysis (MDA), which was used to find Campylobacter in food products with the help of a certain standard method, which is referred to as the reference method. The results of this study showed that the commercial LAMP based method is as efficient as the reference method, and features high specificity and minimum determinability (sensitivity). The LAMP based method has been shown to be a fast and reliable method for detection of Campylobacter spp. scarce presence (10 CFU/25 g) in meat, meat products, as well as carcass swabs and production facilities’ environment. The LAMP analysis required about 24–27 hours to achieve a result. However the LAMP based method will facilitate the detection of Campylobacter, as it provides much easier and faster detection of Cam  pylobacter spp., including Campylobacter jejuni/Campylobacter coli, than standard microbiological methods. The LAMP based method is an efficient tool to prevent the spreading of Campylobacter spp. contamination in food products.
Scattering Amplitudes and BCFW in $\mathcal{N}=2^*$ Theory
Authors: Abhishek Md , Hegde Subramanya , Jatkar Dileep , Saha Arnab
Published: July 2022

We use massive spinor helicity formalism to study scattering
amplitudes in \mathcal{N}=2^*𝒩=2*
super-Yang-Mills theory in four dimensions. We compute the amplitudes at
an arbitrary point in the Coulomb branch of this theory. We compute
amplitudes using projection from \mathcal{N}=4𝒩=4
theory and write three point amplitudes in a convenient form using
special kinematics. We then compute four point amplitudes by carrying
out massive BCFW shift of the amplitudes. We find some of the shifted
amplitudes have a pole at z=\inftyz=∞.
Taking the residue at z=\inftyz=∞
into account ensures little group covariance of the final result.
HÜZÜNLÜ MÜZİKTEN HOŞLANMA: DUYGUDURUM DÜZENLEME STRATEJİLERİ VE KİŞİLİK ÖZELLİKLERİNİN YORDAYICI ETKİSİ
Authors: ARMAN KALKANDELER Ayşe
Published: July 2022

Araştırma kapsamında öncelikle Müzikle Duygudurum Düzenleme Ölçeği - Kısa Formu’nun Türkiye örneklemi için geçerlik ve güvenirlik çalışması 296 kişilik bir örneklem ile yapılmıştır. Doğrulayıcı faktör analizi sonucunda, ölçeğin orijinal yedi faktörlü yapısının veriyle uyumlu olduğu belirlenmiştir. Ayrıca alt boyutlara ait iç tutarlılık katsayılar da kabul edilebilir düzeydedir. Araştırmanın ikinci kısmında, Türkçe Büyük Beşli Kişilik Envanteri, Müzikle Duygudurum Düzenleme Ölçeği - Kısa Formu ve Hüzünlü Müziği Beğenme Ölçeği kullanılarak, kişilik özellikleri ve duygudurum düzenleme stratejilerinin hüzünlü müzikten hoşlanma üzerindeki yordayıcı etkisi belirlenmiştir. Buna göre, dışadönüklük ve duygusal denge kişilik özellikleri ile dikkati dağıtma, serbest bırakma, bilişsel değerlendirme ve teselli bulma stratejilerinin hüzünlü müzikten hoşlanmayı yordadığı belirlenmiştir. Buna göre dinleyiciler, hüzün duygusuyla sağlıklı bir şekilde başa çıkabilmek için, farklı duygudurum düzenleme stratejilerini hüzünlü müzikler aracılığıyla kullanıyor gibi görünmektedir.
İSTANBUL ÜNİVERSİTESİ NADİR ESERLER KÜTÜPHANESİ T. 5526 NUMARADA KAYITLI DÎVÂN-I İLHÂMÎ NÜSHASI TEZHİBİ
Authors: KARAHAN DALBAŞ Ebru
Published: July 2022

Osmanlı Devleti XVII. yüzyıl sonlarında Avrupa devletleri karşısında aldığı askerî yenilgiler sonrasında XVIII. yüzyıl başlarından itibaren Batı’yla ilişiklerini sistematik hâle getirmeye başlamıştır. “Batılılaşma” olarak adlandırılan hareketler Sultan III. Selim Dönemi’nde (1789-1807) daha bilinçli bir şekilde sürdürülmüş, özellikle askerî sahadaki yeniliklerin izleri, toplumsal ve siyasi alanda olduğu kadar kültür ve sanatta da seçilir olmuştur. Yeniliklerden nasibini alan tezhip sanatında da farklı uygulamalar yer bulmuştur. Barok ve rokoko üsluplarının Türk zevki ile birleşmesi neticesinde “Türk rokokosu” olarak adlandırılan üslupta eserler verilmiştir. Bununla beraber klasik kaideler motif, desen ve işçilikteki kayıplara rağmen uygulanmaya devam etmiştir. Bu makaleye konu olan el yazması eser, İstanbul Üniversitesi Nadir Eserler Kütüphânesi T. 5526 numarada kayıtlı Dîvân-ı İlhâmî nüshasıdır. Dönemine göre tezyinatı özenli bir şekilde yapılmış klasik usuldeki eserlerden olan yazma, bulunduğu kütüphanede sayfa sayfa incelenmiş, eb’adı, bezemeli sayfaları tespit edilmiştir. Nitel araştırma yönteminin kullanıldığı araştırmada eserin künye bilgileri verilmiş, bezemeli sayfaları desen analizi yapılarak ayrıntılı bir şekilde anlatılmıştır.
Evaluation of effective parameters of Manning roughness coefficients in HDPE culverts via kernel-based approaches
Authors: Nassaji Matin Ghazaleh
Published: July 2022

The prediction of Manning coefficients plays a prominent role in the estimation of head losses along culvert systems. Although the Manning coefficient is treated as a constant, previous studies showed the dependency of this coefficient on several parameters. This study aims to evaluate the effective parameters of the Manning roughness coefficient using intelligence approaches such as Gaussian process regression (GPR) and support vector machines (SVM), in which the input variables were considered as dimensionless and dimensional. In addition to the enhanced efficiency of the SVM approach compared to the GPR approach in model development with dimensionless input variables, the accuracy of model A(I) with input parameters of Fr (Froude) and y/D (the ratio of water depth to culvert diameter) and performance criteria of correlation coefficient (R) = 0.738, determination coefficient (DC) = 0.0962, root mean square errors (RMSE) = 0.0015 and R = 0.818, DC = 0.993 and RMSE = 0.0006 for GPR and SVM approaches were the highest. Thus, for the second category, a model with an input parameter of discharge (Q), hydraulic radius (RH), and culvert's slope (S0) showed good efficiency in predicting the Manning coefficient, in which the performance criteria of GPR and SVM approaches were (R = 0.719, DC = 0.949, RMSE = 0.0013) and (R = 0.742, DC = 0.991, RMSE = 0.007), respectively. Furthermore, developed OAT (one-at-a-time) sensitivity analysis revealed that relative depth y/D and Q are the most important parameters in the prediction of the Manning coefficient for models with dimensionless and dimensional input variables, respectively.
Domestic water demand prediction based on system dynamics combined with social-hydrology methods
Authors: Chen Lu , Gan Xiaoxue , Yi Bin , Qin Yehongping , Lu Lanqing
Published: July 2022

Systematic domestic water demand prediction is of great importance for social and economic sustainable development. To explain the system of domestic water demand prediction more completely, a system dynamic model based on the social-hydrology method was proposed in this paper. The key natural and socio-economic factors that affect the domestic water demand most were identified and investigated. The prediction equations of water price considering the feedback between humans and nature were deduced. The expressions of water-saving consciousness were proposed for the first time. Furthermore, the differential and difference equations for domestic water demand prediction were established to realize the future domestic water demand forecasts considering the natural and social driving forces. Finally, three scenarios were designed to investigate the impact of climate change and economic development on the domestic water demand. The upper and middle Pearl River Basin was selected as a case study. The predicted domestic water demand is consistent with the historical results, which demonstrates that the proposed model can be well used for the prediction of the domestic water demand. Under the three scenarios, the domestic water demand increased by 33, 27 and 18% in 2050 compared with that in 2017, respectively.
Fatores de riscos para lesão por pressão x Estratégias de prevenção: Interfaces do cuidado de enfermagem no âmbito hospitalar
Authors: Ribeiro Wanderson Alves , Dias Lilian Laine da Conceição , Santos LarissaChristiny Amorim dos , Fassarella Bruna Porath Azevedo , Alves Ana Lúcia Naves , Neves Keila do Carmo , Amaral Fernando Salgado do
Published: July 2022

A importância da prevenção de lesões por pressão nos cuidados com o adulto se dá em proporcionar meios conclusivos para manter a integridade da pele durante sua permanência em ambiente hospitalar e durante a assistência domiciliar. É notório que a formação de lesão por pressão é um agravante à saúde, pois permite que outras patologias sejam associadas prejudicando o bem-estar físico do paciente. Trata-se de um estudo do tipo pesquisa bibliográfica, através de meio eletrônico pela Biblioteca Virtual de Saúde (BVS), nas seguintes bases de dados: Literatura Latino-Americana e do Caribe em Ciências da Saúde (Lilacs), Base de Dados de Enfermagem (Bdenf) e Medical Literature Analysis and Retrieval System Online (Medline). A partir deste estudo, concluiu-se que ao se tratar de métodos preventivos, foi evidenciado a qualidade da assistência de enfermagem, através da elaboração de cuidados de enfermagem com o uso de materiais e equipamentos adequados ao alívio da lesão nas proeminências ósseas e aos cuidados específicos com a pele. Ressaltando assim, o papel fundamental da enfermagem no incentivo e apoio, constituindo assim, autonomia para reconhecerem e empregarem os meios preventivos que poderão ser utilizados na prevenção da lesão por pressão.
Palavras-chave: Cuidados de Enfermagem; Lesão por Pressão; Pele.
Os efeitos da educação interprofissional na prática em saúde
Authors: Mendes Fernanda de Almeida Jannuzzi , Figueira Lucas Garcia da Rosa , Silva Marcos Alex Mendes da
Published: July 2022

Introdução: a educação interprofissional (EIP) é uma das muitas estratégias destinadas a enfrentar a crise da força de trabalho em saúde, considerando que poucos profissionais de saúde foram expostos à mesma durante a sua formação acadêmica ou vivenciaram a prática colaborativa. A trajetória da incorporação da EIP nos sistemas de saúde não aconteceu de forma linear e uniforme na última década, influenciada por conceitos polissêmicos para uma mesma prática. Objetivo: o presente estudo tem como objetivo conhecer os efeitos da EIP nos sistemas de saúde disponíveis na literatura científica mundial. Método: trata-se de uma revisão integrativa e atualizada, cuja busca nas bases de dados Pubmed, Scielo e LILACS ocorreu no ano de 2021 com os descritores em saúde “Interprofessionality and health services”. Os critérios de inclusão adotados foram artigos com no máximo 5 anos de publicação; artigos completos ou resumos que levassem aos artigos completos; artigos do tipo revisão de literatura, sistemática e publicações qualitativas, cujo escopo estivesse alinhado ao objetivo do estudo. Foram incialmente selecionadas 196 publicações, das quais 19 foram incorporadas ao estudo. Resultados: as informações demonstraram os efeitos da EIP nos sistemas de saúde em três grandes temas: a cultura organizacional do sistema de saúde, a identidade profissional e a mudança no processo de trabalho. Considerações finais: os efeitos induzidos pela EIP nos sistemas de saúde são a diminuição dos custos operacionais, melhoria no acesso, produção de respostas rápidas aos problemas de saúde, melhora na segurança do paciente, investimento nas tecnologias de comunicação, valorização da empatia coletiva, reafirmação da integralidade, da resolutividade, da satisfação dos usuários e maior conforto dos trabalhadores.
Palavras-chaves: Educação Interprofissional; Atenção à Saúde; Sistemas de Saúde.
Simulação Baseada em Arduino e Supervisório SCADABR De Acionamento e Monitoramento Sob Carga de Disjuntor de Média Tensão em Subestações Blindadas
Authors: Franco Ferreira Alex , Luiz Santos de Almeida Maycon
Published: July 2022

O estudo foi desenvolvido com intuito de utilizar um sistema supervisório e microcontrolador Arduino para monitorar disjuntores que são manobrados sob carga de subestações blindadas, pois este equipamento possui vida útil inferior aos disjuntores que não são utilizados para tal. Contudo, foi necessário escolher um supervisório de custos baixos para implementação e simulação de tais disjuntores, uma programação no Arduino que possibilite a IHM do supervisório comunicar com o processo, resultando no controle dos equipamentos e monitoramento das operações realizadas pelo operador no supervisório e permitindo a contagem de manobras de abertura e fechamento sob carga desses disjuntores.
Estudo da Conversão do Óleo de Mamona (Ricinus communis L) em Polímeros uma Revisão
Authors: Pires Caramez Gonçalves Hanna , De Souza Siqueira Pereira Cristiane , De Carvalho José Thomaz , Souza Teixeira da Silva Hídila
Published: July 2022

A conversão de óleos vegetais em biopolímeros aparece como uma alternativa sustentava promissora na substituição polímeros produzida à base de petróleo. Dentre os demais óleos vegetais já conhecidos, o óleo oriundo da mamona (Ricinus communis L) apresenta um grande potencial de utilização devido à quantidade de ácidos graxos que sua estrutura apresenta especialmente o do ácido ricinoleico. A modificação do ácido ricinoleico possibilitando a conversão do óleo em diferentes polímeros. Sua obtenção é dada, geralmente, pela prensagem a frio e extração com solventes. Posteriormente o óleo é filtrado e refinado visando o aumento da estabilidade. O óleo refinado é encaminhado para as etapas de conversão, onde se inicia a síntese dos polímeros que é combinada pela sequência de operações tais como: desidratação, hidrogenação, pirólise, transesterificação e polimerização. Os polímeros obtidos podem ser classificados como:  poliuretanos, poliamidas e poliésteres, e os que difere são as diferentes propriedades físicas e químicas. Esses polímeros sintetizados podem ser aplicados em diversos setores tanto industriais quanto farmacêuticos. Deste modo, esta revisão apresenta as etapas de produção desses polímeros mais frequentemente utilizadas, desde a extração do óleo como as diferentes aplicações.