bluuebunny/crossref_metadata_2025 huggingface dataset:
A Fast-Response Driving Waveform Design Based on High-Frequency Voltage for Three-Color Electrophoretic Displays
Authors: Zhang Hu , Yi Zichuan , Liu Liming , Chi Feng , Hu Yunfeng , Huang Sida , Miao Yu , Wang Li
Published: December 2021

Three-color electrophoretic displays (EPDs) have the characteristics of colorful display, reflection display, low power consumption, and flexible display. However, due to the addition of red particles, response time of three-color EPDs is increased. In this paper, we proposed a new driving waveform based on high-frequency voltage optimization and electrophoresis theory, which was used to shorten the response time. The proposed driving waveform was composed of an activation stage, a new red driving stage, and a black or white driving stage. The response time of particles was effectively reduced by removing an erasing stage. In the design process, the velocity of particles in non-polar solvents was analyzed by Newton’s second law and Stokes law. Next, an optimal duration and an optimal frequency of the activation stage were obtained to reduce ghost images and improve particle activity. Then, an optimal voltage which can effectively drive red particles was tested to reduce the response time of red particles. Experimental results showed that compared with a traditional driving waveform, the proposed driving waveform had a better performance. Response times of black particles, white particles and red particles were shortened by 40%, 47.8% and 44.9%, respectively.
Calibration Method of Accelerometer Based on Rotation Principle Using Double Turntable Centrifuge
Authors: Dong Xianshan , Huang Xinlong , Du Guizhen , Huang Qinwen , Huang Yixiong , Huang Yun , Lai Ping
Published: December 2021

For linear accelerometers, calibration with a precision centrifuge is a key technology, and the input acceleration imposed on the accelerometer should be accurately obtained in the calibration. However, there are often errors in the installation of sample that make the calibration inaccurate. To solve installation errors and obtain the input acceleration in the calibration of the accelerometer, a calibration method based on the rotation principle using a double turntable centrifuge is proposed in this work. The key operation is that the sub-turntable is rotated to make the input axis of the accelerometer perpendicular to the direction of the centripetal acceleration vector. Models of installation errors of angle and radius were built. Based on these models, the static radius and input acceleration can be obtained accurately, and the calibration of the scale factor, nonlinearity and asymmetry can be implemented. Using this method, measurements of the MEMS accelerometer with a range of ±30 g were carried out. The results show that the discrepancy of performance obtained from different installation positions was smaller than 100 ppm after calibrating the input acceleration. Moreover, the results using this method were consistent with those using the back-calculation method. These results demonstrate that the effectiveness of our proposed method was confirmed. This method can measure the static radius directly eliminating the installation errors of angle and radius, and it simplifies the accelerometer calibration procedure.
Characterizing the Alteration in Rumen Microbiome and Carbohydrate-Active Enzymes Profile with Forage of Muskoxen Rumen through Comparative Metatranscriptomics
Authors: Wu Xiaofeng , Elekwachi Chijioke O. , Bai Shiping , Luo Yuheng , Zhang Keying , Forster Robert J.
Published: December 2021

Muskox (Ovibos moschatus), as the biggest herbivore in the High Arctic, has been enduring the austere arctic nutritional conditions and has evolved to ingest and digest scarce and high lignified forages to support the growth and reproduce, implying probably harbor a distinct microbial reservoir for the deconstruction of plant biomass. Therefore, metagenomics approach was applied to characterize the rumen microbial community and understand the alteration in rumen microbiome of muskoxen fed either triticale straw or brome hay. The difference in the structure of microbial communities including bacteria, archaea, fungi, and protozoa between the two forages was observed at the taxonomic level of genus. Further, although the highly abundant phylotypes in muskoxen rumen fed either triticale straw or brome hay were almost the same, the selective enrichment different phylotypes for fiber degrading, soluble substrates fermenting, electron and hydrogen scavenging through methanogenesis, acetogenesis, propionogenesis, and sulfur-reducing was also noticed. Specifically, triticale straw with higher content of fiber, cellulose selectively enriched more lignocellulolytic taxa and electron transferring taxa, while brome hay with higher nitrogen content selectively enriched more families and genera for degradable substrates-digesting. Intriguingly, the carbohydrate-active enzyme profile suggested an over representation and diversity of putative glycoside hydrolases (GHs) in the animals fed on triticale straw. The majority of the cellulases belonged to fiver GH families (i.e., GH5, GH6, GH9, GH45, and GH48) and were primarily synthesized by Ruminococcus, Piromyces, Neocallimastix, and Fibrobacter. Abundance of major genes coding for hemicellulose digestion was higher than cellulose mainly including GH8, GH10, GH16, GH26, and GH30, and these enzymes were produced by members of the genera Fibrobacter, Ruminococcus, and Clostridium. Oligosaccharides were mainly of the GH1, GH2, GH3, and GH31 types and were associated with the genera Prevotella and Piromyces. Our results strengthen metatranscriptomic evidence in support of the understanding of the microbial community and plant polysaccharide response to changes in the feed type and host animal. The study also establishes these specific microbial consortia procured from triticale straw group can be used further for efficient plant biomass hydrolysis.
High-Throughput Sequencing Analysis of the Composition and Diversity of the Bacterial Community in Cinnamomum camphora Soil
Authors: Chen Deqiang , Sun Weihong , Xiang Shuang , Zou Shuangquan
Published: December 2021

Soil bacterial communities and root-associated microbiomes play important roles in the nutrient absorption and healthy growth of host plants. Cinnamomum camphora is an important timber and special economic forest tree species in Fujian Province. In this study, the high-throughput sequencing technique was used to analyze the composition, diversity, and function of the bacterial communities present in the soil from different samples and slope positions of C. camphora. The results of this analysis demonstrated that the related bacterial communities in C. camphora soil were mainly clustered based on sample type. Bacterial alpha diversity in the rhizosphere and bulk soil of C. camphora growing downhill was higher than that of C. camphora growing uphill. At the phylum level, Bacteroidetes, Proteobacteria, Chloroflexi, and Gemmatimonadetes were positively correlated with pH, available phosphorus, total phosphorus, available potassium, and total potassium, while Acidobacteria and Verrucomicrobia were negatively correlated with alkaline-hydrolyzable nitrogen. These results show that there were remarkable differences in the composition, diversity, and function of related bacterial communities between different sample types of C. camphora soil. The slope position had a marked effect on the bacterial communities in the rhizosphere and bulk soil, while the root endosphere remained unaffected.
New Biocalcifying Marine Bacterial Strains Isolated from Calcareous Deposits and Immediate Surroundings
Authors: Vincent Julia , Colin Béatrice , Lanneluc Isabelle , Sabot René , Sopéna Valérie , Turcry Philippe , Mahieux Pierre-Yves , Refait Philippe , Jeannin Marc , Sablé Sophie
Published: December 2021

Marine bacterial biomineralisation by CaCO3 precipitation provides natural limestone structures, like beachrocks and stromatolites. Calcareous deposits can also be abiotically formed in seawater at the surface of steel grids under cathodic polarisation. In this work, we showed that this mineral-rich alkaline environment harbours bacteria belonging to different genera able to induce CaCO3 precipitation. We previously isolated 14 biocalcifying marine bacteria from electrochemically formed calcareous deposits and their immediate environment. By microscopy and µ-Raman spectroscopy, these bacterial strains were shown to produce calcite-type CaCO3. Identification by 16S rDNA sequencing provided between 98.5 and 100% identity with genera Pseudoalteromonas, Pseudidiomarina, Epibacterium, Virgibacillus, Planococcus, and Bhargavaea. All 14 strains produced carbonic anhydrase, and six were urease positive. Both proteins are major enzymes involved in the biocalcification process. However, this does not preclude that one or more other metabolisms could also be involved in the process. In the presence of urea, Virgibacillus halodenitrificans CD6 exhibited the most efficient precipitation of CaCO3. However, the urease pathway has the disadvantage of producing ammonia, a toxic molecule. We showed herein that different marine bacteria could induce CaCO3 precipitation without urea. These bacteria could then be used for eco-friendly applications, e.g., the formation of bio-cements to strengthen dikes and delay coastal erosion.
Isolation and Characterization of Effective Bacteria That Reduce Ammonia Emission from Livestock Manure
Authors: Kim Sun-Il , Heo Wan , Lee So-Jung , Kim Young-Jun
Published: December 2021

Ammonia from livestock manure reacts with chemical components discharged from various emission sources to produce airborne particulate matter. This study aimed to investigate a novel effective microbial agent to suppress ammonia gas emitted from manure. Both isolated L12I and 12III strains, identified as Pediococcus acidilactici (PA), were selected for their superior activity in assays performed with the evaluation criteria such as acid production, ammonia decomposition, and urease inhibition, which are key factors influencing ammonia excretion. The survivability of PA strains was confirmed by an increase in DNA abundance in the manure. PA strains lowered the pH of manure and suppressed the growth of hyper-ammonia-producing bacteria (HAB) possessing urease activity. The L12I and 12III treatment groups showed 23.58% and 38.00% emission reductions, respectively. Especially, the 12III strain was proven to be the more effective strain for reducing ammonia gas emission, with the best ability to reduce pH and inhibit HAB. The strains could have an additive effect in improving the manure quality as a nitrogen fertilizer by preserving the total nitrogen and urea content. These results suggest that PA strains can be used as unprecedented microbial agents to improve manure-derived environmental pollution and improve fertilizer quality.
Zoisite-(Pb), a New Orthorhombic Epidote-Related Mineral from the Jakobsberg Mine, Värmland, Sweden, and Its Relationships with Hancockite
Authors: Perchiazzi Natale , Mauro Daniela , Vignola Pietro , Zaccarini Federica , Eldjarn Knut
Published: December 2021

The new mineral zoisite-(Pb), ideally CaPbAl3(SiO4)(Si2O7)O(OH), was discovered in a sample from the Jakobsberg manganese-iron oxide deposit, Värmland, Sweden. Zoisite-(Pb) is found as pale pink subhedral prisms elongated on [010], up to 0.3 mm in size, associated with calcite, celsian, diopside, grossular, hancockite, hyalophane, native lead, phlogopite, and vesuvianite. Associated feldspars show one of the highest PbO contents (~7–8 wt%) found in nature. Electron-microprobe analysis of zoisite-(Pb) point to the empirical formula (Ca1.09Pb0.86Mn2+0.01Na0.01)∑1.97(Al2.88Fe3+0.10Mn3+0.04)∑3.02Si3.00O12(OH)1.00. The eight strongest diffraction lines [dobs, Iobs, (hkl)] are 8.63 s (101), 8.11 mw (200), 4.895 m (011), 4.210 m (211), 3.660 s (112, 311), 3.097 mw (312), 2.900 s (013), and 2.725 m (511). Zoisite-(Pb) is isostructural with zoisite and its crystal structure was refined up to R1 = 0.0213 for 2013 reflections with Fo > 4σ(Fo). Pb shows a stereochemically active lone pair leading to a lopsided distribution of its coordinating oxygens. A full chemical and Raman characterization of zoisite-(Pb) and of the Pb-bearing epidote hancockite is reported, together with an improved crystal structural model of hancockite, refined up to R1 = 0.0254 for 2041 reflections with Fo > 4σ(Fo). The effects of the incorporation of Pb in the crystal structure of zoisite-(Pb), hancockite, and related synthetic and natural phases are described and discussed.
The Kaolinite Crystallinity and Influence Factors of Coal-Measure Kaolinite Rock from Datong Coalfield, China
Authors: Liu Dongna , Zhang Yun , Zhou Anchao , Nnachi Emmanuel , Huo Shuting , Zhang Qi
Published: December 2021

In order to ascertain the kaolinite crystallinity of Carboniferous Permian coal-measure kaolinite rocks, seven groups of fresh samples were collected from below the ground in the Xiaoyu mine, Datong coalfield. Microscopy, X-ray diffraction (XRD), differential thermal analysis (DTA), infrared (IR) spectroscopy and X-ray fluorescence (XRF) spectrometry methods were applied to the samples. The petrographic analysis results show that the kaolinite rocks are characterized as compact, phaneritic, clastic, sand-bearing, sandy and silty types; the kaolinite content in the Shanxi formation and upper Taiyuan formations was more than 95%, while it was 60–90% in the middle and lower Taiyuan formations. Based on the Hinckley index and the features of XRD, DTA and IR of kaolinites, crystallinity was classified as having three grades: ordered, slightly disordered and disordered. The kaolinites’ SiO2/Al2O3 molar ratio was about 1.9–5.7, with a chemical index of alteration (CIA) of about 95.4–99.5. This research suggests that the kaolinite crystallinity correlates positively to its clay mineral content, purity and particle size, which are also related to the SiO2/Al2O3 molar ratio and CIA. The original sedimentary environment and weathering have a direct influence on kaolinite crystallinity, and the existence of organic matter is conducive to the stable existence of kaolinite. The study results have significance for the extraction and utilization of coal-measure kaolinite and the development of kaolinite crystallography and mineralogy.
Biosynthesis and Characterization of Extracellular Silver Nanoparticles from Streptomyces aizuneusis: Antimicrobial, Anti Larval, and Anticancer Activities
Authors: Abd-Elhady Hemmat M. , Ashor Mona A. , Hazem Abdelkader , Saleh Fayez M. , Selim Samy , El Nahhas Nihal , Abdel-Hafez Shams H. , Sayed Samy , Hassan Enas A.
Published: December 2021

The ability of microorganisms to reduce inorganic metals has launched an exciting eco-friendly approach towards developing green nanotechnology. Thus, the synthesis of metal nanoparticles through a biological approach is an important aspect of current nanotechnology. In this study, Streptomyces aizuneusis ATCC 14921 gave the small particle of silver nanoparticles (AgNPs) a size of 38.45 nm, with 1.342 optical density. AgNPs produced by Streptomyces aizuneusis were characterized by means of UV-VIS spectroscopy and transmission electron microscopy (TEM). The UV-Vis spectrum of the aqueous solution containing silver ion showed a peak between 410 to 430. Moreover, the majority of nanoparticles were found to be a spherical shape with variables between 11 to 42 nm, as seen under TEM. The purity of extracted AgNPs was investigated by energy dispersive X-ray analysis (EDXA), and the identification of the possible biomolecules responsible for the reduction of Ag+ ions by the cell filtrate was carried out by Fourier Transform Infrared spectrum (FTIR). High antimicrobial activities were observed by AgNPs at a low concentration of 0.01 ppm, however, no deleterious effect of AgNPs was observed on the development and occurrence of Drosophila melanogaster phenotype. The highest reduction in the viability of the human lung carcinoma and normal cells was attained at 0.2 AgNPs ppm.
Phyto-Functionalized Silver Nanoparticles Derived from Conifer Bark Extracts and Evaluation of Their Antimicrobial and Cytogenotoxic Effects
Authors: Macovei Irina , Luca Simon Vlad , Skalicka-Woźniak Krystyna , Sacarescu Liviu , Pascariu Petronela , Ghilan Alina , Doroftei Florica , Ursu Elena-Laura , Rimbu Cristina Mihaela , Horhogea Cristina Elena , Lungu Cristina , Vochita Gabriela , Panainte Alina Diana , Nechita Constantin , Corciova Maria Andreia , Miron Anca
Published: December 2021

Silver nanoparticles synthesized using plant extracts as reducing and capping agents showed various biological activities. In the present study, colloidal silver nanoparticle solutions were produced from the aqueous extracts of Picea abies and Pinus nigra bark. The phenolic profile of bark extracts was analyzed by liquid chromatography coupled to mass spectrometry. The synthesis of silver nanoparticles was monitored using UV-Vis spectroscopy by measuring the Surface Plasmon Resonance band. Silver nanoparticles were characterized by attenuated total reflection Fourier transform infrared spectroscopy, Raman spectroscopy, dynamic light scattering, scanning electron microscopy, energy dispersive X-ray and transmission electron microscopy analyses. The antimicrobial and cytogenotoxic effects of silver nanoparticles were evaluated by disk diffusion and Allium cepa assays, respectively. Picea abies and Pinus nigra bark extract derived silver nanoparticles were spherical (mean hydrodynamic diameters of 78.48 and 77.66 nm, respectively) and well dispersed, having a narrow particle size distribution (polydispersity index values of 0.334 and 0.224, respectively) and good stability (zeta potential values of −10.8 and −14.6 mV, respectively). Silver nanoparticles showed stronger antibacterial, antifungal, and antimitotic effects than the bark extracts used for their synthesis. Silver nanoparticles obtained in the present study are promising candidates for the development of novel formulations with various therapeutic applications.
Insights into the Protective Effects of Thymoquinone against Toxicities Induced by Chemotherapeutic Agents
Authors: Farooq Juveriya , Sultana Rokeya , Taj Tahreen , Asdaq Syed Mohammed Basheeruddin , Alsalman Abdulkhaliq J. , Mohaini Mohammed Al , Al Hawaj Maitham A. , Kamal Mehnaz , Alghamdi Saad , Imran Mohd. , Shahin Haleema , Tabassum Ruheena
Published: December 2021

The drugs used to treat cancer not only kill fast-growing cancer cells, but also kill or slow the growth of healthy cells, causing systemic toxicities that lead to altered functioning of normal cells. Most chemotherapeutic agents have serious toxicities associated with their use, necessitating extreme caution and attention. There is a growing interest in herbal remedies because of their pharmacological activities, minimal side effects, and low cost. Thymoquinone, a major component of the volatile oil of Nigella sativa Linn, also known as black cumin or black seeds, is commonly used in Middle Eastern countries as a condiment. It is also utilized for medicinal purposes and possesses antidiabetic, anti-cancer, anti-inflammatory, hepatoprotective, anti-microbial, immunomodulatory, and antioxidant properties. This review attempts to compile the published literature demonstrating thymoquinone’s protective effect against chemotherapeutic drug-induced toxicities.
Cloning, Expression and Inhibitory Effects on Lewis Lung Carcinoma Cells of rAj-Tspin from Sea Cucumber (Apostichopus japonicus)
Authors: Qiao Rong , Xiao Rong , Chen Zhong , Jiang Jingwei , Yuan Chenghua , Ning Shuxiang , Wang Jihong , Zhou Zunchun
Published: December 2021

In recent years, sea cucumber has become a favorite healthcare food due to its characteristic prevention of cardiovascular diseases, suppression of tumors, as well as enhancement of immunity. In order to screen the anti-tumoral proteins or peptides from sea cucumber (Apostichopus japonicus), its cDNA library was analyzed, and a disintegrin-like and metalloproteinase with thrombospondin type 1 motif, member 13 (ADAMTS13)-like was found. ADAMTS13-like contains 10 thrombospondin 1 (TSP1) domains. Based on analysis of bioinformatics, the third TSP1 domain of this protein, which is further named Aj-Tspin, contains an arginine-glycine-aspartate (RGD) motif. Since our previous studies showed that the recombinant RGD-containing peptide from lampreys showed anti-tumoral activity, the third TSP1 domain of ADAMTS13-like was chosen to evaluate it’s effect on tumor proliferation and metastasis, despite the fact it shares almost no homologue with disintegrins from other species. After artificial synthesis, its cDNA sequence, Aj-Tspin, which is composed of 56 amino acids, was subcloned into a pET23b vector and expressed as a recombinant Aj-Tspin (rAj-Tspin) in a soluble form with a molecular weight of 6.976 kDa. Through affinity chromatography, rAj-Tspin was purified as a single protein. Both anti-proliferation and immunofluorescence assays showed that rAj-Tspin suppressed the proliferation of Lewis lung carcinoma (LLC) cells through apoptosis. Adhesion assay also displayed that rAj-Tspin inhibited the adhesion of LLC cells to ECM proteins, including fibronectin, laminin, vitronectin and collagen. Lastly, rAj-Tspin also suppressed the migration and invasion of LLC cells across the filter in transwells. Thus, the above indicates that rAj-Tspin might act as a potential anti-tumoral drug in the future and could also provide information on the nutritional value of sea cucumber.
Phase Structure and Electrical Properties of Sm-Doped BiFe0.98Mn0.02O3 Thin Films
Authors: Wang Yangyang , Li Zhaoyang , Ma Zhibiao , Wang Lingxu , Guo Xiaodong , Liu Yan , Yao Bingdong , Zhang Fengqing , Zhu Luyi
Published: December 2021

Bi1−xSmxFe0.98Mn0.02O3 (x = 0, 0.02, 0.04, 0.06; named BSFMx) (BSFM) films were prepared by the sol-gel method on indium tin oxide (ITO)/glass substrate. The effects of different Sm content on the crystal structure, phase composition, oxygen vacancy content, ferroelectric property, dielectric property, leakage property, leakage mechanism, and aging property of the BSFM films were systematically analyzed. X-ray diffraction (XRD) and Raman spectral analyses revealed that the sample had both R3c and Pnma phases. Through additional XRD fitting of the films, the content of the two phases of the sample was analyzed in detail, and it was found that the Pnma phase in the BSFMx = 0 film had the lowest abundance. X-ray photoelectron spectroscopy (XPS) analysis showed that the BSFMx = 0.04 film had the lowest oxygen vacancy content, which was conducive to a decrease in leakage current density and an improvement in dielectric properties. The diffraction peak of (110) exhibited the maximum intensity when the doping amount was 4 mol%, and the minimum leakage current density and a large remanent polarization intensity were also observed at room temperature (2Pr = 91.859 μC/cm2). By doping Sm at an appropriate amount, the leakage property of the BSFM films was reduced, the dielectric property was improved, and the aging process was delayed. The performance changes in the BSFM films were further explained from different perspectives, such as phase composition and oxygen vacancy content.
A Novel Method to Prepare Transparent, Flexible and Thermally Conductive Polyethylene/Boron Nitride Films
Authors: Yi Mingming , Han Meng , Chen Junlin , Hao Zhifeng , Chen Yuanzhou , Yao Yimin , Sun Rong
Published: December 2021

The high thermal conductivity and good insulating properties of boron nitride (BN) make it a promising filler for high-performance polymer-based thermal management materials. An easy way to prepare BN-polymer composites is to directly mix BN particles with polymer matrix. However, a high concentration of fillers usually leads to a huge reduction of mechanical strength and optical transmission. Here, we propose a novel method to prepare polyethylene/boron nitride nanoplates (PE/BNNPs) composites through the combination of electrostatic self-assembly and hot pressing. Through this method, the thermal conductivity of the PE/BNNPs composites reach 0.47 W/mK, which gets a 14.6% improvement compared to pure polyethylene film. Thanks to the tight bonding of polyethylene with BNNPs, the tensile strength of the composite film reaches 1.82 MPa, an increase of 173.58% compared to that of pure polyethylene film (0.66 MPa). The fracture stress was also highly enhanced, with an increase of 148.44% compared to pure polyethylene film. Moreover, the addition of BNNPs in PE does not highly reduce its good transmittance, which is preferred for thermal management in devices like light-emitting diodes. This work gives an insight into the preparation strategy of transparent and flexible thermal management materials with high thermal conductivity.
Polarization-Insensitive Beam Splitter with Variable Split Angles and Ratios Based on Phase Gradient Metasurfaces
Authors: He Quan , Shen Zhe
Published: December 2021

The beam splitter is a common and critical element in optical systems. Traditional beam splitters composed of prisms or wave plates are difficult to be applied to miniaturized optical systems because they are bulky and heavy. The realization of the nanoscale beam splitter with a flexible function has attracted much attention from researchers. Here, we proposed a polarization-insensitive beam splitter with a variable split angle and ratio based on the phase gradient metasurface, which is composed of two types of nanorod arrays with opposite phase gradients. Different split angles are achieved by changing the magnitude of the phase gradient based on the principle of Snell’s law of refraction, and different split ratios are achieved by adding a phase buffer with different areas. In the designed four types of beam splitters for different functions, the split angle is variable in the range of 12–29°, and the split ratio is variable in the range of 0.1–1. The beam splitter has a high beam splitting efficiency above 0.3 at the wavelength of 480–600 nm and a weak polarization dependence. The proposed beam splitter has the advantages of a small size and easy integration, and it can be applied to various optical systems such as multiplexers and interferometers for integrated optical circuits.
Structural and Electrochemical Properties of Physically and Chemically Activated Carbon Nanoparticles for Supercapacitors
Authors: Alhebshi Nuha A. , Salah Numan , Hussain Humair , Salah Yousef N. , Yin Jian
Published: December 2021

The demand for supercapacitors has been high during the integration of renewable energy devices into the electrical grid. Although activated carbon materials have been widely utilized as supercapacitor electrodes, the need for economic and sustainable processes to extract and activate carbon nanomaterials is still crucial. In this work, the biomass waste of date palm fronds is converted to a hierarchical porous nanostructure of activated carbon using simple ball-milling and sonication methods. Chemical and physical activation agents of NaOH and CO2, receptively, were applied on two samples separately. Compared with the specific surface area of 603.5 m2/g for the CO2-activated carbon, the NaOH-activated carbon shows a higher specific surface area of 1011 m2/g with a finer nanostructure. Their structural and electrochemical properties are functionalized to enhance electrode–electrolyte contact, ion diffusion, charge accumulation, and redox reactions. Consequently, when used as electrodes in an H2SO4 electrolyte for supercapacitors, the NaOH-activated carbon exhibits an almost two-fold higher specific capacitance (125.9 vs. 56.8 F/g) than that of the CO2-activated carbon at the same current density of 1 A/g. Moreover, using carbon cloth as a current collector provides mechanical flexibility to our electrodes. Our practical approach produces cost-effective, eco-friendly, and flexible activated carbon electrodes with a hierarchical porous nanostructure for supercapacitor applications.
Naltrexone Implant for Opioid Use Disorder
Authors: Edinoff Amber N. , Nix Catherine A. , Orellana Claudia V. , StPierre Samantha M. , Crane Erin A. , Bulloch Blaine T. , Cornett Elyse M. , Kozinn Rachel L. , Kaye Adam M. , Murnane Kevin S. , Kaye Alan D.
Published: December 2021

The continued rise in the availability of illicit opioids and opioid-related deaths in the United States has left physicians, researchers, and lawmakers desperate for solutions to this ongoing epidemic. The research into therapeutic options for the treatment of opioid use disorder (OUD) began with the introduction of methadone in the 1960s. The approval of oral naltrexone initially showed much promise, as the drug was observed to be highly potent in antagonizing the effects of opioids while producing no opioid agonist effects of its own and having a favorable side effect profile. Patients that routinely take their naltrexone reported fewer days of heroin use and had more negative drug tests than those without treatment. Poor outcomes in OUD patients treated with naltrexone have been directly tied to short treatment time. Studies have shown that naltrexone given orally vs. as an implant at the 6-month interval showed a higher non-compliance rate among those who used oral medications at the 6-month mark and a slower return to use rate. There were concerns that naltrexone could possibly worsen negative symptoms seen in opiate use disorder related to blockade of endogenous opioids that are important for pleasurable stimuli. Studies have shown that naltrexone demonstrated no increase in levels of anxiety, depression and anhedonia in participants and another study found that those treated with naltrexone had a significant reduction in mental health-related hospitalizations. The latter study also concluded that there was no increased risk for mental health-related incidents in patients taking naltrexone via a long-acting implant. Although not yet FDA approved in the United States, naltrexone implant has shown promising results in Europe and Australia and may provide a novel treatment option for opioid addiction.
The Nutritional Profile and On-Pack Marketing of Toddler-Specific Food Products Launched in Australia between 1996 and 2020
Authors: McCann Jennifer R. , Russell Catherine G. , Woods Julie L.
Published: December 2021

With the food system evolving, it is not clear how the nutrition and on-pack claims of toddler foods have been impacted. Data on the trends in Australia are lacking, so we sought to determine the changes in the nutrition and on-pack claims of toddler-specific packaged foods over time. A retrospective cross-sectional analysis was conducted using the Mintel Global New Products Database. The number of toddler-specific foods increased from 1996 to 2020. Over time, a lower proportion of meals and snacks were classified as “ultra-processed”, but a higher proportion of snacks were classified as “discretionary”. Meals launched after 2014 had higher median values for energy, saturated fat, and sugar than those in earlier years. Toddler snacks launched after 2014 had lower median values for sodium, and higher median values for fat, saturated fat, and sugar than those in earlier years. The mean number of total claims per package increased over time for snacks, with an increase in unregulated claims for both meals and snacks. Public health action is needed to ensure that the retail food environment for young children is health-promoting, including stringent and clear regulations for on-pack claims, and compositional guidelines and guidance on how to reduce the number of ultra-processed foods for toddlers.
Dietary Acid Load and Relationship with Albuminuria and Glomerular Filtration Rate in Individuals with Chronic Kidney Disease at Predialysis State
Authors: Silva Luísa , Moço Sara Alegria , Antunes Maria Luz , Ferreira Andreia Sousa , Moreira Ana Catarina
Published: December 2021

The Western diet, characterized by excessive consumption of animal protein and reduced intake of vegetables and fruits, is also rich in sulfur, chlorine, and organic acids, which are the main sources of dietary acid load. A relationship between dietary acid load, renal function, and progression of chronic kidney disease has been demonstrated. Dietary modifications seem to contribute to a reduction in dietary acid load, and are associated with improved outcomes in individuals with chronic kidney disease (CKD). The aim of this paper was to review the existing evidence concerning the association between dietary acid load and renal function in nondialyzed individuals with CKD. A systematic review was conducted by gathering articles in electronic databases (MEDLINE/PubMed, Scopus, and Web of Science) from January 2018 to May 2021. Dietary acid load and GFR and/or albuminuria were analyzed. A total of 1078 articles were extracted, of which 5 met the inclusion criteria. Only one study found no statistically significant associations between the study variables. The remaining showed a negative association between dietary acid load and renal function. This systematic review confirmed the existence of an association between dietary acid load and renal function, with a high dietary acid load contributing to a decreased renal function.
Core Needle Biopsy Enhances the Activity of the CCL2/CCR2 Pathway in the Microenvironment of Invasive Breast Cancer
Authors: Heiskala Marja , Joensuu Kristiina , Heikkilä Päivi
Published: December 2021

The use of core needle biopsy (CNB) as a means to verify malignancy preoperatively is a paradigm in current breast cancer care, and the risk of enhancing tumor development by this procedure has been considered insignificant. Experimental work in mice has shown preoperative biopsies to increase tumor supportive elements in the microenvironment, whereas, in humans, the impact of CNB on the host’s immunologic response has not been investigated. In this pilot study, we compared the expression of CCL2/CCR2 pathway components at the protein level in samples from CNBs to those from the corresponding resected tumors from 52 patients with primary breast cancer. We found an increased expression of CD163, CD14 and CCR2 in monocytes/macrophages and a slight decrease of CCL2 in the malignant epithelium in the tumors after the biopsy. The increased infiltration of immunosuppressive monocytes/macrophages and the decreased tumor cell CCL2 expression, presumably due to the CCR2 availability-dependent CCL2 internalization, suggest that CNB enhances the activity of the CCL2/CCR2 pathway, and this finding warrants confirmatory examination. The switch in the context-dependent role of CCL2 on the polarization of macrophages may lead to increased tumor supportive function both locally and in the peripheral immune machinery. The future directions in breast cancer should include early interventions to support the tumor surveillance of the host.
The Neuroactive Steroid Pregnanolone Glutamate: Anticonvulsant Effect, Metabolites and Its Effect on Neurosteroid Levels in Developing Rat Brains
Authors: Kudova Eva , Mares Pavel , Hill Martin , Vondrakova Katerina , Tsenov Grygoriy , Chodounska Hana , Kubova Hana , Vales Karel
Published: December 2021

Pregnanolone glutamate (PA-G) is a neuroactive steroid that has been previously demonstrated to be a potent neuroprotective compound in several biological models in vivo. Our in vitro experiments identified PA-G as an inhibitor of N-methyl-D-aspartate receptors and a potentiator of γ-aminobutyric acid receptors (GABAARs). In this study, we addressed the hypothesis that combined GABAAR potentiation and NMDAR antagonism could afford a potent anticonvulsant effect. Our results demonstrated the strong age-related anticonvulsive effect of PA-G in a model of pentylenetetrazol-induced seizures. PA-G significantly decreased seizure severity in 12-day-old animals, but only after the highest dose in 25-day-old animals. Interestingly, the anticonvulsant effect of PA-G differed both qualitatively and quantitatively from that of zuranolone, an investigational neurosteroid acting as a potent positive allosteric modulator of GABAARs. Next, we identified 17-hydroxy-pregnanolone (17-OH-PA) as a major metabolite of PA-G in 12-day-old animals. Finally, the administration of PA-G demonstrated direct modulation of unexpected neurosteroid levels, namely pregnenolone and dehydroepiandrosterone sulfate. These results suggest that compound PA-G might be a pro-drug of 17-OH-PA, a neurosteroid with a promising neuroprotective effect with an unknown mechanism of action that may represent an attractive target for studying perinatal neural diseases.
Plant Photochemistry, Reactive Oxygen Species, and Photoprotection
Authors: Moustakas Michael
Published: December 2021

Light energy, absorbed as photons by chlorophylls and other pigment molecules consisting of light-harvesting complexes (LHCs), is transferred to the reaction centres (RCs), where, through charge separation, electrons flow from photosystem II (PSII) through cytochrome b6f and diffusible electron carriers to photosystem I (PSI) [...]
Fabrication and Characterization of Superhydrophobic Graphene/Titanium Dioxide Nanoparticles Composite
Authors: Wu Xun Hui , Then Yoon Yee
Published: December 2021

Materials with superhydrophobic surfaces have received vast attention in various industries due to their valuable properties, such as their self-cleaning and antifouling effects. These promising superhydrophobic properties are taken into high priority, particularly for medical devices and applications. The development of an ideal superhydrophobic surface is a challenging task and is constantly progressing. Various strategies have been introduced; however, a minority of them are cost-effective. This work presents a facile fabrication of the superhydrophobic surface by using graphene and titanium dioxide (TiO2) nanoparticles. The graphene and TiO2 hybrid nanoparticles are dip-coated on a biodegradable thermoplastic poly(lactic acid) (PLA) substrate. The thermoplastic PLA is approved by the Food and Drug Administration (FDA), and is widely utilized in medical devices. The graphene/TiO2 coating is substantiated to transform the hydrophilic PLA film into superhydrophobic biomaterials that can help to reduce hazardous medical-device complications. The surface wettability of the graphene/TiO2 nanoparticle-coated PLA surface was evaluated by measuring the apparent water contact angle. The surface chemical composition and surface morphology were analyzed via Fourier-transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). The graphene/TiO2-coated PLA film achieved superhydrophobic properties by demonstrating a water contact angle greater than 150°. The water contact angle of the graphene/TiO2 coating increased along with the concentration of the nanoparticles and the ratio of TiO2 to graphene. Moreover, the graphene/TiO2 coating exhibited excellent durability, whereby the contact angle of the coated surface remained unchanged after water immersion for 24 h. The duration of the effectiveness of the superhydrophobic coating suggests its suitability for medical devices, for which a short duration of administration is involved. This study reports an easy-to-replicate and cost-effective method for fabricating superhydrophobic graphene/TiO2-coated surfaces, which additionally substantiates a potential solution for the manufacturing of biomaterials in the future.
Supercritical Carbon Dioxide Cycles for Concentrated Solar Power Plants: A Possible Alternative for Solar Desalination
Authors: González-Almenara Rafael , Rodríguez de Arriba Pablo , Crespi Francesco , Sánchez David , Muñoz Antonio , Sánchez-Lencero Tomás
Published: December 2021

This manuscript investigates the supercritical carbon dioxide (sCO2) power cycle employed in the power block of concentrated solar power (CSP) plants—solar tower—as an alternative for solar desalination, developed with either distillation or reverse osmosis. This concept is investigated as a possible up-scaling of the SOLMIDEFF project, originally based on a hot-air micro gas turbine combined with a solar dish collector. For the upscaled concept, five different sCO2 cycles are considered, chosen amongst the best-performing configurations proposed in the literature for CSP applications, and modelled with Thermoflex software. The influence of ambient conditions is studied, considering two minimum cycle temperatures (35 °C and 50 °C), corresponding to Santa Cruz de Tenerife and Abu Dhabi, respectively. The results show that the low temperatures at the inlet of the heat rejection unit compromise the viability of distillation technologies. On the other hand, the high thermal efficiency achieved by these cycles, especially with the recompression and partial cooling layouts, reduces the specific energy consumption when combined with reverse osmosis (RO), below that of photovoltaic (PV)+RO. Feed-water preheating is explored as a solution to further reduce energy consumption, concluding that its actual interest is not clear and strongly depends on the location considered and the corresponding water quality standards.
Barriers and Enablers for Continuous Improvement Methodologies within the Irish Pharmaceutical Industry
Authors: McDermott Olivia , Antony Jiju , Sony Michael , Daly Stephen
Published: December 2021

This study aims to investigate the barriers that exist when implementing continuous improvement methodologies, such as Lean Six Sigma (LSS), within the Irish Pharma industry. The main finding of this study is that 45% of participants perceived that a highly regulated environment could be a barrier to continuous improvement implementation, while 97% of respondents utilised Continuous improvement (CI) methods, such as Lean, Six Sigma, and LSS, within their organisations. While the International Conference of Harmonisation integrates CI into its Pharmaceutical Quality Systems (PQS) regulations, the highest motivation for CI implementation amongst the Irish Pharma industry is to improve Productivity and Quality. The main obstacles highlighted for CI implementation in Pharma attributed to stringent regulatory regimes were fear of extra validation activity, a compliance versus quality culture, and a regulatory culture of being “safe”. Another relevant finding presented in this paper is that participants CI LSS tools are very strongly integrated into the pharma industries corrective and preventative action system, deviations, and internal audit systems. Limitations of the research are that all the data collected in the survey came from professionals working for multinational Pharmaceutical companies based in Ireland. The authors understand that this is the first research focused on the barriers and status of CI initiatives in the pharmaceutical industry. The results of this study represent an important step towards understanding the enablers and obstacles for the use of continuous improvement methodologies in pharmaceutical manufacturing industries on a global scale.
Improving Leaf Area Index Retrieval Using Multi-Sensor Images and Stacking Learning in Subtropical Forests of China
Authors: Chen Yang , Ma Lixia , Yu Dongsheng , Feng Kaiyue , Wang Xin , Song Jie
Published: December 2021

The leaf area index (LAI) is a key indicator of the status of forest ecosystems that is important for understanding global carbon and water cycles as well as terrestrial surface energy balances and the impacts of climate change. Machine learning (ML) methods offer promising ways of generating spatially explicit LAI data covering large regions based on optical images. However, there have been few efforts to analyze the LAI in heterogeneous subtropical forests with complex terrain by fusing high-resolution multi-sensor data from the Sentinel-1 Synthetic Aperture Radar (SAR), Sentinel-2 Multi Spectral Instrument (MSI), and Advanced Land Observing Satellite-1 digital elevation model (DEM). Here, forest LAI mapping was performed by integrating the MSI, SAR, and DEM data using a stacking learning (SL) approach that incorporates distinct predictions from a set of optimized individual ML algorithms. The method’s performance was evaluated by comparison to field forest LAI measurements acquired in Xingguo and Gandong of subtropical China. The results showed that the addition of the SAR and DEM images using the SL model compared to the inputs of only optical images reduced the mean absolute error (MAE) and root mean square error (RMSE) by 26% and 18%, respectively, in Xingguo, and by 12% and 8%, respectively, in Gandong. Furthermore, the combination of all images had the best prediction performance. SL was found to be more robust and accurate than conventional individual ML models, while the MAE and RMSE were decreased by 71% and 64%, respectively, in Xingguo, and by 68% and 59%, respectively, in Gandong. Therefore, the SL model using the three-source data combination produced satisfied prediction accuracy with the coefficients of determination (R2), MAE, and RMSE of 0.96, 0.17, and 0.28, respectively, in Xingguo and 0.94, 0.30, and 0.47, respectively, in Gandong. This study revealed the potential of the SL algorithm for retrieving the forest LAI using multi-sensor data in areas with complex terrain.
Multiple Effects of Topographic Factors on Spatio-Temporal Variations of Vegetation Patterns in the Three Parallel Rivers Region, Southeast Qinghai-Tibet Plateau
Authors: Wang Chunya , Wang Jinniu , Naudiyal Niyati , Wu Ning , Cui Xia , Wei Yanqiang , Chen Qingtao
Published: December 2021

Topographic factors are critical for influencing vegetation distribution patterns, and studying the interactions between them can enhance our understanding of future vegetation dynamics. We used the Moderate-resolution Imaging Spectroradiometer Normalized Differential Vegetation Index (MODIS NDVI) image dataset (2000–2019), combined with the Digital Elevation Model (DEM), and vegetation type data for trend analysis, and explored NDVI variation and its relationship with topographic factors through an integrated geographically-weighted model in the Three Parallel Rivers Region (TPRR) of southeastern Qinghai-Tibet Plateau (QTP) in the past 20 years. Our results indicated that there was no significant increase of NDVI in the entire basin between 2000–2019, except for the Lancang River basin. In the year 2004, abrupt changes in NDVI were observed across the entire basin and each sub-basin. During 2000–2019, the mean NDVI value of the whole basin increased initially and then decreased with the increasing elevation. However, it changed marginally with variations in slope and aspect. We observed a distinct spatial heterogeneity in vegetation patterns with elevation, with higher NDVI in the southern regions NDVI than those in the north as a whole. Most of the vegetation cover was concentrated in the slope range of 8~35°, with no significant difference in distribution except flat land. Furthermore, from 2000 to 2019, the vegetation cover in the TPRR showed an improving trend with the changes of various topographic factors, with the largest improvement area (36.10%) in the slightly improved category. The improved region was mainly distributed in the source area of the Jinsha River basin and the southern part of the whole basin. Geographically weighted regression (GWR) analysis showed that elevation was negatively correlated with NDVI trends in most areas, especially in the middle reaches of Nujiang River basin and Jinsha River basin, where the influence of slope and aspect on NDVI change was considerably much smaller than elevation. Our results confirmed the importance of topographic factors on vegetation growth processes and have implications for understanding the sustainable development of mountain ecosystems.