bluuebunny/crossref_metadata_2025 huggingface dataset:
Connecting Central America to the Southern Cone: The Chilean and Argentine Response to the Nicaraguan Revolution of 1979
Authors: Avery Molly
Published: October 2021

The history of the Cold War in Latin America in the 1970s is commonly split into two episodes: the establishment of anticommunist dictatorships and the ensuing repression across the Southern Cone in the early and middle decade, and the Nicaraguan Revolution and the eruption of violent conflicts across Central America at its close. By exploring the Chilean and Argentine response to the Nicaraguan Revolution, this article brings these two episodes together, demonstrating how they were understood to belong to one and the same ideological conflict. In doing so, it highlights the importance of the revolution in the Chilean and Argentine perception of the Cold War and explores how the Sandinista triumph directly shaped Southern Cone ideas about US power and the communist threat, also prompting reflection on their own ‘models’ for anticommunist governance. Both regimes responded by increasing their support for anticommunist forces in Guatemala and El Salvador, often conducting this aid through a wider transnational and clandestine network. This article contributes to new understandings of the nature of Latin American anticommunism in this period, challenges traditional understandings of external involvement in Central America, and demonstrates the need to understand events in Latin America in this period in their full regional context.
Perceptual Load and Sex-Specific Personality Traits
Authors: Lange-Küttner Christiane , Puiu Andrei-Alexandru
Published: October 2021

Abstract. The impact of sex-specific personality traits has often been investigated for visuospatial tasks such as mental rotation, but less is known about the influence of personality traits on visual search. We investigated whether the Big Five personality traits Extroversion (E), Openness (O), Agreeableness (A), Conscientiousness (C), and Neuroticism (N) and the Autism Quotient (AQ) influence visual search in a sample of N = 65 men and women. In three experiments, we varied stimulus complexity and predictability. As expected, latencies were longer when the target was absent. Pop-out search was faster than conjunction search. A large number of distracters slowed down reaction times (RTs). When stimulus complexity was not predictable in Experiment 3, this reduced search accuracy by about half. As could be predicted based on previous research on long RT tails, conjunction search in target absent trials revealed the impact of personality traits. The RT effect in visual search of the accelerating “less social” AQ score was specific to men, while the effects of the “more social” decelerating Big Five Inventory factors agreeableness and conscientiousness were specific to women. Thus, sex-specific personality traits could explain decision-making thresholds, while visual stimulus complexity yielded an impact of the classic personality traits neuroticism and extroversion.
Where and How the East Madagascar Current Retroflection Originates?
Authors: Ramanantsoa Juliano D. , Penven P. , Raj R. P. , Renault L. , Ponsoni L. , Ostrowski M. , Dilmahamod A. F. , Rouault M.
Published: October 2021

The East Madagascar Current (EMC) is one of the western boundary currents of the South Indian Ocean. As such, it plays an important role in the climate system by transporting water and heat toward the pole and recirculating to the large‐scale Indian Ocean through retroflection modes of its southern extension. Five cruise data sets and remote sensing data from different sensors are used to identify three states of the southern extension of the EMC: early retroflection, canonical retroflection, and no retroflection. Retroflections occur 47% of the time. EMC strength regulates the retroflection state, although impinged mesoscale eddies also contribute to retroflection formation. Early retroflection is linked with EMC volume transport. Anticyclonic eddies drifting from the central Indian Ocean to the coast favor early retroflection formation, anticyclonic eddies near the southern tip of Madagascar promote the generation of canonical retroflection, and no retroflection appears to be associated with a lower eddy kinetic energy (EKE). Knowledge of the EMC retroflection state could help predict (a) coastal upwelling south of Madagascar, (b) the southeastern Madagascar phytoplankton bloom, and (c) the formation of the South Indian Ocean Counter Current (SICC).
Drivers of Global Clear Sky Surface Downwelling Longwave Irradiance Trends From 1984 to 2017
Authors: Clark J. P. , Clothiaux E. E. , Feldstein S. B. , Lee S.
Published: October 2021

Radiation changes at the Earth's surface alter climate, however, the causes of observed surface radiation changes are not precisely quantified globally. With complete global coverage by ERA‐Interim, the drivers of the clear sky surface downwelling longwave irradiance (SDLI) trends from 1984 to 2017 are quantifiable everywhere. Trends in atmospheric temperature and water vapor contributed significantly (∼90%) to clear sky SDLI trends, including trends consistent with Arctic warming and Southern Ocean cooling. CO2 contributed ∼10% and other greenhouse gases (CH4, N2O, CFC‐11, and CFC‐12) ∼1% to the SDLI trends. These observation‐based results are consistent with early CO2‐doubling climate model calculations wherein temperature and water vapor changes drove ∼90% of the SDLI change. The well‐mixed greenhouse gases drive location‐dependent SDLI trends that are strongest over regions with climatologically high temperatures and low water vapor amounts.
Interannual Variability of Surface Salinity and Ekman Pumping in the Canada Basin During Summertime of 2003–2017
Authors: Dong Jihai , Jin Meibing , Liu Yu , Dong Changming
Published: October 2021

The Canada Basin (CB) is undergoing significant changes due to long‐term global climate change, and other shorter time‐scale processes that are still not well understood. Based on the in‐situ and satellite observations and atmospheric reanalysis data, interannual variabilities of sea surface salinity and Ekman pumping in the CB are investigated in the summertime of 2003–2017. This study reveals strong surface salinity reduction and Ekman downwelling enhancement during 2006–2012 compared with the remaining years. The surface 20‐m salinity averaged in 2006–2012 decreases by 1.26 PSU relative to the other years. The results suggest that the lower surface salinity is correlated to enhanced sea ice melt and Ekman convergence in the CB. Enhanced downward solar radiation accounts for the accelerated sea ice melt over the southeast CB, leading to the surface salinity decrease. Strengthened anticyclonic wind circulation over the CB not only enhances sea ice melt via strengthening offshore Ekman transport over the Chukchi Sea slope, but also favors freshwater accumulation in the CB through strengthening the horizontal convergence. The sea ice melt is estimated to account for 54% of the surface freshening, and the horizontal convergence is believed to play an important role in the remaining 46%. During the freshening years in the CB, the Ekman downwelling enhancement is mainly attributed to variations of wind stress curl, sea ice concentration, and ice‐ocean stress curl, and their relative contributions are estimated on the basis of on a linearized model as 72%, 12%, and 10%, respectively.
Slower Long‐Term Coastal Warming Drives Dampened Trends in Coastal Marine Heatwave Exposure
Authors: Marin Maxime , Bindoff Nathaniel L. , Feng Ming , Phillips Helen E.
Published: October 2021

Long‐term temperature changes drive coastal Marine Heatwave (MHW) trends globally. Here, we provide a more comprehensive global analysis of cross‐shore gradients of MHW and Sea Surface Temperatures (SST) changes using an ensemble of three satellite SST products during recent decades. Our analysis reveals depressed onshore SST trends in more than 2/3 of coastal pixels, including both eastern and western boundary current systems. These were well correlated with depressed trends of MHW exposure and severity, ranging from a −2 to −10 decrease in MHW days per decade and from a −2.5°C to −15°C.days per decade decrease in cumulative intensity. Results were consistent across all satellite products, indicating that these cross‐shore gradients are a robust feature of observations. ERA reanalysis data show that neither air‐sea heat fluxes nor wind driven upwelling were found to be consistent drivers. Global ocean circulation models (OFAM3 and ACCESS‐OM2) have limited ability to simulate the depressed onshore trends. A heat budget analysis performed in the Chilean coast region, where models agree with observations, showed that the gradient of temperature change was controlled by an onshore increase of longwave radiative cooling, despite an increase in upwelling. This highlights the complexity of small‐scale coastal ocean‐atmosphere feedbacks, which coarser resolution climate models do not resolve. Here, we show that global coastal regions may act as thermal refugia for marine ecosystems from aspects of climate change and pulsative (MHW) changes. Contrary to the literature, our results suggest that driving mechanisms are region dependant, stressing the necessity to improve climate models resolution.
Non‐Marine Sources Contribute to Aerosol Methanesulfonate Over Coastal Seas
Authors: Zhou Shengqian , Chen Ying , Paytan Adina , Li Haowen , Wang Fanghui , Zhu Yucheng , Yang Tianjiao , Zhang Yan , Zhang Ruifeng
Published: October 2021

Methanesulfonate (MSA) in the marine boundary layer is commonly considered to be solely contributed by the oxidation of ocean‐derived dimethyl sulfide (DMS) and often used as an indicator of marine biogenic sources. But whether this judgment is valid in coastal seas and how the validity is affected by air mass transport history have been less discussed. Based on multi‐year observations of aerosol MSA in the coastal East China Sea (ECS) and the Gulf of Aqaba (GA), as well as the analysis of air mass transport pattern and exposure to ocean surface phytoplankton biomass, we found that terrestrial sources made a non‐negligible contribution to MSA over the ECS but not over the GA. The abundant MSA in winter over the coastal ECS was likely associated with substantial emissions of volatile organic sulfur compounds from both anthropogenic and natural sources in eastern China and significant terrestrial transport influenced by the East Asian Monsoon. Good correlations between aerosol MSA and air mass exposure to surface phytoplankton biomass were established by removing the influence of terrestrial transport and confining the air transport height within boundary layer, which makes it possible to construct parameterizations for obtaining the spatiotemporal distributions of marine biogenic aerosol components using satellite ocean color datasets.
Rock Slope Temperature Evolution and Micrometer‐Scale Deformation at a Retreating Glacier Margin
Authors: Hugentobler Marc , Aaron Jordan , Loew Simon
Published: October 2021

In deglaciating environments, rock mass weakening and potential formation of rock slope instabilities is driven by long‐term and seasonal changes in thermal‐ and hydraulic‐ boundary conditions, combined with unloading due to ice melting. However, in‐situ observations are rare. In this study, we present new monitoring data from three highly instrumented boreholes, and numerical simulations to investigate rock slope temperature evolution and micrometer‐scale deformation during deglaciation. Our results show that the subsurface temperatures are adjusting to a new, warmer surface temperature following ice retreat. Heat conduction is identified as the dominant heat transfer process at sites with intact rock. Observed non‐conductive processes are related to groundwater exchange with cold subglacial water, snowmelt infiltration, or creek water infiltration. Our strain data shows that annual surface temperature cycles cause thermoelastic deformation that dominate the strain signals in the shallow thermally active layer at our stable rock slope locations. At deeper sensors, reversible strain signals correlating with pore pressure fluctuations dominate. Irreversible deformation, which we relate with progressive rock mass damage, occurs as short‐term (hours to weeks) strain events and as slower, continuous strain trends. The majority of the short‐term irreversible strain events coincides with precipitation events or pore pressure changes. Longer‐term trends in the strain time series and a minority of short‐term strain events cannot directly be related to any of the investigated drivers. We propose that the observed increased damage accumulation close to the glacier margin can significantly contribute to the long‐term formation of paraglacial rock slope instabilities during multiple glacial cycles.
Convection‐Permitting Simulations With the E3SM Global Atmosphere Model
Authors: Caldwell P. M. , Terai C. R. , Hillman B. , Keen N. D. , Bogenschutz P. , Lin W. , Beydoun H. , Taylor M. , Bertagna L. , Bradley A. M. , Clevenger T. C. , Donahue A. S. , Eldred C. , Foucar J. , Golaz J.‐C. , Guba O. , Jacob R. , Johnson J. , Krishna J. , Liu W. , Pressel K. , Salinger A. G. , Singh B. , Steyer A. , Ullrich P. , Wu D. , Yuan X. , Shpund J. , Ma H.‐Y. , Zender C. S.
Published: October 2021

This paper describes the first implementation of the Δx = 3.25 km version of the Energy Exascale Earth System Model (E3SM) global atmosphere model and its behavior in a 40‐day prescribed‐sea‐surface‐temperature simulation (January 20 through February 28, 2020). This simulation was performed as part of the DYnamics of the Atmospheric general circulation Modeled On Non‐hydrostatic Domains (DYAMOND) Phase 2 model intercomparison. Effective resolution is found to be the horizontal dynamics grid resolution despite using a coarser grid for physical parameterizations. Despite this new model being in an immature and untuned state, moving to 3.25 km grid spacing solves several long‐standing problems with the E3SM model. In particular, Amazon precipitation is much more realistic, the frequency of light and heavy precipitation is improved, agreement between the simulated and observed diurnal cycle of tropical precipitation is excellent, and the vertical structure of tropical convection and coastal stratocumulus look good. In addition, the new model is able to capture the frequency and structure of important weather events (e.g., tropical cyclones, extratropical cyclones including atmospheric rivers, and cold air outbreaks). Interestingly, this model does not get rid of the erroneous southern branch of the intertropical convergence zone nor the tendency for strongest convection to occur over the Maritime Continent rather than the West Pacific, both of which are classic climate model biases. Several other problems with the simulation are identified, underscoring the fact that this model is a work in progress.
Representations of Precipitation Diurnal Cycle in the Amazon as Simulated by Observationally Constrained Cloud‐System Resolving and Global Climate Models
Authors: Tai Sheng‐Lun , Feng Zhe , Ma Po‐Lun , Schumacher Courtney , Fast Jerome D.
Published: October 2021

The ability of an observationally‐constrained cloud‐system resolving model (Weather Research and Forecasting; WRF, 4‐km grid spacing) and a global climate model (Energy Exascale Earth System Model; E3SM, 1‐degree grid spacing) to represent the precipitation diurnal cycle over the Amazon basin during the 2014 wet season is assessed. The WRF model coupled with a 3‐D variational data assimilation scheme reproduces the spatial variability of the precipitation diurnal cycle over the basin and the lifecycle of westward propagating MCSs initiated by the coastal sea‐breeze front. In contrast, a single morning peak in rainfall is produced by E3SM for simulations despite the nudging of large‐scale winds toward global reanalysis, indicating precipitation in E3SM is largely controlled by local convection associated with diurnal heating. The role of propagating MCS on the environment are discussed by using a multivariate perturbation analysis. We also find that the advection of moisture perturbations from ocean to inland regions have a higher correlation with the occurrence of MCSs in the Amazon than the intensity of colder air intrusion associated with sea breezes along the coast. Moreover, the presence of large cold pools over the central Amazon basin are responsible for the maintenance of propagating deep convection.
Anti-reflux medication use in preterm infants
Authors: Binti Abdul Hamid Haslina , Szatkowski Lisa , Budge Helen , Ojha Shalini
Published: October 2021

Background

Current recommendations do not support the use of anti-reflux medications to treat gastro-oesophageal reflux disease (GORD) among preterm infants.

Objective

To describe the prevalence of GORD and the use of anti-reflux medications amongst very preterm infants (<32 weeks’ gestational age (GA)) in neonatal units in England and Wales.

Design

Retrospective cohort study using the National Neonatal Research Database.

Results

Among 58,108 infants [median GA (IQR) 29 (27–30) weeks], 15.8% (n = 9191) had a diagnosis of GORD and 36.9% (n = 12,446) received anti-reflux medications. Those who received anti-reflux medications were more preterm [GA, median (IQR): medications, 28 (26–30) vs. no medications, 30 (28–31); p < 0.001] and had lower birth weight [mean (SD): medications, 1124 g (354) vs. no medications, 1265 g (384); p < 0.001]. Most (57%, n = 12,224) received Gaviscon, or Histamine-2 Receptor Antagonist (H2RA) (56%, n = 11,959). Over time, prokinetic use has declined substantially, the use of H2RAs and Gaviscon has reduced although they continue to be used frequently, whilst the use of PPIs has increased.

Conclusions

Anti-reflux medications are frequently prescribed in very preterm infants, despite evidence to suggest that they are not effective and may be harmful. Clear guidelines for diagnosing GORD and the use of anti-reflux medications are required to rationalise the pharmacological management of GORD in preterm infants.

Impact

Anti-reflux medications are frequently prescribed, often without a diagnosis of gastro-oesophageal reflux disease, to very preterm infants while in the neonatal unit and at discharge.

Half of the infants born at <28 weeks’ gestational age receive anti-reflux medications in hospital and a quarter are discharged home on them.

Although the use of prokinetics declined following alerts of adverse events, histamine2-receptor antagonists and alginates such as Gaviscon continue to be used and the use of proton-pump inhibitors has increased more than 2-fold.
CircETFA upregulates CCL5 by sponging miR-612 and recruiting EIF4A3 to promote hepatocellular carcinoma
Authors: Lu Chen , Rong Dawei , Hui Bingqing , He Xuezhi , Jiang Wei , Xu Yetao , Cao Hongyong , Xu Zekuan , Tang Weiwei
Published: October 2021

As a kind of malignant tumors, hepatocellular carcinoma (HCC) has been studied continuously, but the mechanisms are not well understood. Circular RNAs (circRNAs) are widespread in eukaryotes and play an important role in the growth of organisms and in the occurrence of diseases. The role of circRNAs in HCC remains to be further explored. In this study, CircRNA microarray analysis was used to assess the plasma from HCC patients and healthy controls and to identify circRNAs involved in HCC tumorigenesis. CircETFA was overexpressed in HCC tissues, plasma, and cells. Clinicopathological data revealed that abnormally high circETFA expression was associated with a poor prognosis. In function, circETFA promotes the malignant phenotype of HCC cells in vivo and in vitro, inhibits cycle arrest, and decreases the proportion of apoptotic cells. In mechanism, it can upregulate C-C motif chemokine ligand 5 (CCL5) in HCC cells, thereby regulating the phosphoinositide 3-kinase (PI3K)/Akt pathway and other key downstream effectors (e.g., FoxO6). Furthermore, circETFA prolonged the half-life of CCL5 mRNA by recruiting the eukaryotic initiation factor 4A3 (EIF4A3) and acted as a sponge of hsa-miR-612 to suppress the silencing effect of hsa-miR-612 on CCL5. In conclusion, CircETFA can increase the expression of CCL5 to promote the progression of HCC by sponging hsa-mir-612 and recruiting EIF4A3, and is promising as a novel biomarker and therapeutic target.
Targeting CK2 in cancer: a valuable strategy or a waste of time?
Authors: Salvi Mauro , Borgo Christian , Pinna Lorenzo A. , Ruzzene Maria
Published: October 2021

CK2 is a protein kinase involved in several human diseases (ranging from neurological and cardiovascular diseases to autoimmune disorders, diabetes, and infections, including COVID-19), but its best-known implications are in cancer, where it is considered a pharmacological target. Several CK2 inhibitors are available and clinical trials are underway in different cancer types. Recently, the suitability of CK2 as a broad anticancer target has been questioned by the finding that a newly developed compound, named SGC-CK2-1, which is more selective than any other known CK2 inhibitor, is poorly effective in reducing cell growth in different cancer lines, prompting the conclusion that the anticancer efficacy of CX-4945, the commonly used clinical-grade CK2 inhibitor, is to be attributed to its off-target effects. Here we perform a detailed scrutiny of published studies on CK2 targeting and a more in-depth analysis of the available data on SGC-CK2-1 vs. CX-4945 efficacy, providing a different perspective about the actual reliance of cancer cells on CK2. Collectively taken, our arguments would indicate that the pretended dispensability of CK2 in cancer is far from having been proved and warn against premature conclusions, which could discourage ongoing investigations on a potentially valuable drug target.
Wip1 regulates the immunomodulatory effects of murine mesenchymal stem cells in type 1 diabetes mellitus via targeting IFN-α/BST2
Authors: Zhou Na , Liu Weijiang , Zhang Wei , Liu Yuanlin , Li Xue , Wang Yang , Zheng Rongxiu , Zhang Yi
Published: October 2021

Mesenchymal stem cells (MSCs) show significant therapeutic effects in type 1 diabetes mellitus (T1DM) as regulating the inflammatory processes. However, little is known about the detailed process of MSCs immunosuppression in T1DM. In this study, we investigated the effects of wild-type p53-induce phosphatase 1 (Wip1) on regulating MSCs immunosuppressive capacities in T1DM mice. We found that Wip1 knockout (Wip1−/−) MSCs had lower therapeutic effects in T1DM mice, and displayed weaker immunosuppressive capability. In vivo distribution analysis results indicated that

Wip1−/−MSCs could home to the damaged pancreas and increase the expression of tumor necrosis factor-α (TNF-α), interleukin-17a (IL-17a), interferon-α(IFN-α), IFN-β, and IFN-γ, while decrease the expression of IL-4 and IL-10. Moreover, we confirmed

Wip1−/−MSCs exhibited weaker immunosuppressive capacity, as evidenced by enhanced expression of bone marrow stromal cell antigen 2(BST2) and IFN-α. In conclusion, these results revealed Wip1 affects MSCs immunomodulation by regulating the expression of IFN-α/BST2. Our study uncovered that Wip1 is required to regulate the therapeutic effects of MSCs on T1DM treatment, indicating a novel role of Wip1 in MSCs immunoregulation properties.
Prediction of thermoelectric performance for layered IV-V-VI semiconductors by high-throughput ab initio calculations and machine learning
Authors: Gan Yu , Wang Guanjie , Zhou Jian , Sun Zhimei
Published: October 2021

Layered IV-V-VI semiconductors have immense potential for thermoelectric (TE) applications due to their intrinsically ultralow lattice thermal conductivity. However, it is extremely difficult to assess their TE performance via experimental trial-and-error methods. Here, we present a machine-learning-based approach to accelerate the discovery of promising thermoelectric candidates in this chalcogenide family. Based on a dataset generated from high-throughput ab initio calculations, we develop two highly accurate-and-efficient neural network models to predict the maximum ZT (ZTmax) and corresponding doping type, respectively. The top candidate, n-type Pb2Sb2S5, is successfully identified, with the ZTmax over 1.0 at 650 K, owing to its ultralow thermal conductivity and decent power factor. Besides, we find that n-type Te-based compounds exhibit a combination of high Seebeck coefficient and electrical conductivity, thereby leading to better TE performance under electron doping than hole doping. Whereas p-type TE performance of Se-based semiconductors is superior to n-type, resulting from large Seebeck coefficient induced by high density-of-states near valence band edges.
White matter and nigral alterations in multiple system atrophy-parkinsonian type
Authors: Ogawa Takashi , Hatano Taku , Kamagata Koji , Andica Christina , Takeshige-Amano Haruka , Uchida Wataru , Kamiyama Daiki , Shimo Yasushi , Oyama Genko , Umemura Atsushi , Iwamuro Hirokazu , Ito Masanobu , Hori Masaaki , Aoki Shigeki , Hattori Nobutaka
Published: October 2021

Multiple system atrophy (MSA) is classified into two main types: parkinsonian and cerebellar ataxia with oligodendrogliopathy. We examined microstructural alterations in the white matter and the substantia nigra pars compacta (SNc) of patients with MSA of parkinsonian type (MSA-P) using multishell diffusion magnetic resonance imaging (dMRI) and myelin sensitive imaging techniques. Age- and sex-matched patients with MSA-P (n = 21,n = 10 first and second cohorts, respectively), Parkinson’s disease patients (n = 19, 17), and healthy controls (n = 20, 24) were enrolled. Magnetization transfer saturation imaging (MT-sat) and dMRI were obtained using 3-T MRI. Measurements obtained from diffusion tensor imaging (DTI), free-water elimination DTI, neurite orientation dispersion and density imaging (NODDI), and MT-sat were compared between groups. Tract-based spatial statistics analysis revealed differences in diffuse white matter alterations in the free-water fractional volume, myelin volume fraction, and intracellular volume fraction between the patients with MSA-P and healthy controls, whereas free-water and MT-sat differences were limited to the middle cerebellar peduncle in comparison with those with Parkinson’s disease. Region-of-interest analysis of white matter and SNc revealed significant differences in the middle and inferior cerebellar peduncle, pontine crossing tract, corticospinal tract, and SNc between the MSA-P and healthy controls and/or Parkinson’s disease patients. Our results shed light on alterations to brain microstructure in MSA.
A curated diverse molecular database of blood-brain barrier permeability with chemical descriptors
Authors: Meng Fanwang , Xi Yang , Huang Jinfeng , Ayers Paul W.
Published: October 2021

The highly-selective blood-brain barrier (BBB) prevents neurotoxic substances in blood from crossing into the extracellular fluid of the central nervous system (CNS). As such, the BBB has a close relationship with CNS disease development and treatment, so predicting whether a substance crosses the BBB is a key task in lead discovery for CNS drugs. Machine learning (ML) is a promising strategy for predicting the BBB permeability, but existing studies have been limited by small datasets with limited chemical diversity. To mitigate this issue, we present a large benchmark dataset, B3DB, complied from 50 published resources and categorized based on experimental uncertainty. A subset of the molecules in B3DB has numerical log BB values (1058 compounds), while the whole dataset has categorical (BBB+ or BBB−) BBB permeability labels (7807). The dataset is freely available at https://github.com/theochem/B3DB and https://doi.org/10.6084/m9.figshare.15634230.v3 (version 3). We also provide some physicochemical properties of the molecules. By analyzing these properties, we can demonstrate some physiochemical similarities and differences between BBB+ and BBB− compounds.
Hypothalamic administration of sargahydroquinoic acid elevates peripheral thermogenic signaling and ameliorates high fat diet-induced obesity through the sympathetic nervous system
Authors: Kim Doyeon , Lee Yuna , Kim Hyeung-Rak , Park Yeo Jin , Hwang Hongik , Rhim Hyewhon , Kang Taek , Choi Chun Whan , Lee Bonggi , Kim Min Soo
Published: October 2021

Sargassum serratifolium (C. Agardh) C.Agardh, a marine brown alga, has been consumed as a food and traditional medicine in Asia. A previous study showed that the meroterpenoid-rich fraction of an ethanolic extract of S. serratifolium (MES) induced adipose tissue browning and suppressed diet-induced obesity and metabolic syndrome when orally supplemented. Sargahydroquinoic acid (SHQA) is a major component of MES. However, it is unclear whether SHQA regulates energy homeostasis through the central nervous system. To examine this, SHQA was administrated through the third ventricle in the hypothalamus in high-fat diet-fed C57BL/6 mice and investigated its effects on energy homeostasis. Chronic administration of SHQA into the brain reduced body weight without a change in food intake and improved metabolic syndrome-related phenotypes. Cold experiments and biochemical analyses indicated that SHQA elevated thermogenic signaling pathways, as evidenced by an increase in body temperature and UCP1 signaling in white and brown adipose tissues. Peripheral denervation experiments using 6-OHDA indicated that the SHQA-induced anti-obesity effect is mediated by the activation of the sympathetic nervous system, possibly by regulating genes associated with sympathetic outflow and GABA signaling pathways. In conclusion, hypothalamic injection of SHQA elevates peripheral thermogenic signaling and ameliorates diet-induced obesity.
M1-like macrophage contributes to chondrogenesis in vitro
Authors: Miyamoto Yoshiyuki , Kubota Keigo , Asawa Yukiyo , Hoshi Kazuto , Hikita Atsuhiko
Published: October 2021

Cartilage tissues have poor self-repairing abilities. Regenerative medicine can be applied to recover cartilage tissue damage in the oral and maxillofacial regions. However, hitherto it has not been possible to predict the maturity of the tissue construction after transplantation or to prepare mature cartilage tissues before transplantation that can meet clinical needs. Macrophages play an important role in cartilage tissue regeneration, although the exact mechanisms remain unknown. In this study, we established and verified an in vitro experimental system for the direct co-culture of cell pellets prepared from mouse auricular chondrocytes and macrophages polarized into four phenotypes (M1-like, M1, M2-like, and M2). We demonstrate that cartilage pellets co-cultured with M1-like promoted collagen type 2 and aggrecan production and induced the most significant increase in chondrogenesis. Furthermore, M1-like shifted to M2 on day 7 of co-culture, suggesting that the cartilage pellet supplied factors that changed the polarization of M1-like. Our findings suggest that cartilage regenerative medicine will be most effective if the maturation of cartilage tissues is induced in vitro by co-culture with M1-like before transplantation.
Deep learning-based thin-section MRI reconstruction improves tumour detection and delineation in pre- and post-treatment pituitary adenoma
Authors: Lee Da Hyun , Park Ji Eun , Nam Yeo Kyung , Lee Joonsung , Kim Seonok , Kim Young-Hoon , Kim Ho Sung
Published: October 2021

Even a tiny functioning pituitary adenoma could cause symptoms; hence, accurate diagnosis and treatment are crucial for management. However, it is difficult to diagnose a small pituitary adenoma using conventional MR sequence. Deep learning-based reconstruction (DLR) using magnetic resonance imaging (MRI) enables high-resolution thin-section imaging with noise reduction. In the present single-institution retrospective study of 201 patients, conducted between August 2019 and October 2020, we compared the performance of 1 mm DLR MRI with that of 3 mm routine MRI, using a combined imaging protocol to detect and delineate pituitary adenoma. Four readers assessed the adenomas in a pairwise fashion, and diagnostic performance and image preferences were compared between inexperienced and experienced readers. The signal-to-noise ratio (SNR) was quantitatively assessed. New detection of adenoma, achieved using 1 mm DLR MRI, was not visualised using 3 mm routine MRI (overall: 6.5% [13/201]). There was no significant difference depending on the experience of the readers in new detections. Readers preferred 1 mm DLR MRI over 3 mm routine MRI (overall superiority 56%) to delineate normal pituitary stalk and gland, with inexperienced readers more preferred 1 mm DLR MRI than experienced readers. The SNR of 1 mm DLR MRI was 1.25-fold higher than that of the 3 mm routine MRI. In conclusion, the 1 mm DLR MRI achieved higher sensitivity in the detection of pituitary adenoma and provided better delineation of normal pituitary gland than 3 mm routine MRI.
Screw stripping and its prevention in the hexagonal socket of 3.5-mm titanium locking screws
Authors: Lee Hyo-Jin , Park Young Uk , Kim Sung Jae , Kim Hyong Nyun
Published: October 2021

There have been several reports about the difficulties in removing 3.5-mm titanium locking screws from plates due to the stripping or rounding of the hexagonal screw socket. We investigated whether stripping the locking screw sockets can be prevented by using different screwdrivers or interposing materials into the socket during removal. We overtightened 120 3.5-mm titanium locking screws (Depuy Synthes, Paoli, PA) equally into locking plates on sawbone tibia models, applying a uniform torque of 4.5 Nm, exceeding the recommended torque of 1.5 Nm. Twenty screws each were removed using a straight-handle 2.5-mm screwdriver, T-handle screwdriver, hex key wrench, and straight-handle screwdriver with a non-dominant hand. In addition, 20 screws were removed using foil from a suture packet inserted into the screw socket or using parts of a latex glove inserted into the screw socket. The incidence rates of screw stripping using the straight-handle screwdriver, T-handle screwdriver, hex key wrench, non-dominant hand, foil interposition, and latex glove interposition were 75%, 40%, 35%, 90%, 60%, and 70%, respectively. When a T-handle screwdriver or hex key wrench was used, the probability of screw stripping was 4.50 times (odds ratio = 4.50, 95% confidence interval = 1.17 to 17.37, p = 0.03) and 5.57 times (odds ratio = 5.57, 95% confidence interval = 1.42 to 21.56, p = 0.01) lower than that with the straight-handle screwdriver, respectively. Foil or latex glove interpositions did not prevent screw stripping. Thus, in the current experimental study, T-handle screwdriver or hex key wrench usage decreased the incidence rate of screw stripping during removal compared to straight-handle screwdriver use.
Bioaccumulation and detoxification of trivalent arsenic by Achromobacter xylosoxidans BHW-15 and electrochemical detection of its transformation efficiency
Authors: Diba Farzana , Khan Md. Zaved Hossain , Uddin Salman Zahir , Istiaq Arif , Shuvo Md. Sadikur Rahman , Ul Alam A. S. M. Rubayet , Hossain M. Anwar , Sultana Munawar
Published: October 2021

Arsenotrophic bacteria play an essential role in lowering arsenic contamination by converting toxic arsenite [As (III)] to less toxic and less bio-accumulative arsenate [As (V)]. The current study focused on the qualitative and electrocatalytic detection of the arsenite oxidation potential of an arsenite-oxidizing bacteria A. xylosoxidans BHW-15 (retrieved from As-contaminated tube well water), which could significantly contribute to arsenic detoxification, accumulation, and immobilization while also providing a scientific foundation for future electrochemical sensor development. The minimum inhibitory concentration (MIC) value for the bacteria was 15 mM As (III). Scanning Electron Microscopy (SEM) investigation validated its intracellular As uptake capacity and demonstrated a substantial association with the MIC value. During the stationary phase, the strain’s As (III) transformation efficiency was 0.0224 mM/h. Molecular analysis by real-time qPCR showed arsenite oxidase (aioA) gene expression increased 1.6-fold in the presence of As (III) compared to the untreated cells. The immobilized whole-cell also showed As (III) conversion up to 18 days. To analyze the electrochemical oxidation in water, we developed a modified GCE/P-Arg/ErGO-AuNPs electrode, which successfully sensed and quantified conversion of As (III) into As (V) by accepting electrons; implying a functional As oxidase enzyme activity in the cells. To the best of our knowledge, this is the first report on the electrochemical observation of the As-transformation mechanism with Achromobactersp. Furthermore, the current work highlighted that our isolate might be employed as a promising candidate for arsenic bioremediation, and information acquired from this study may be helpful to open a new window for the development of a cost-effective, eco-friendly biosensor for arsenic species detection in the future.
Resistance status of lepidopteran soybean pests following large-scale use of MON 87701 × MON 89788 soybean in Brazil
Authors: Horikoshi Renato J. , Bernardi Oderlei , Godoy Daniela N. , Semeão Altair A. , Willse Alan , Corazza Gustavo O. , Ruthes Elderson , Fernandes Davi de S. , Sosa-Gómez Daniel R. , Bueno Adeney de F. , Omoto Celso , Berger Geraldo U. , Corrêa Alberto S. , Martinelli Samuel , Dourado Patrick M. , Head Graham
Published: October 2021

Widespread adoption of MON 87701 × MON 89788 soybean, expressing Cry1Ac Bt protein and glyphosate tolerance, has been observed in Brazil. A proactive program was implemented to phenotypically and genotypically monitor Cry1Ac resistance in Chrysodeixis includens (Walker). Recent cases of unexpected injury in MON 87701 × MON 89788 soybean were investigated and a large-scale sampling of larvae on commercial soybean fields was performed to assess the efficacy of this technology and the distribution of lepidopteran pests in Brazil. No significant shift in C. includens susceptibility to Cry1Ac was observed eight years after commercial introduction of this technology in Brazil. F2 screen results confirmed that the frequency of Cry1Ac resistance alleles remains low and stable in C. includens. Unexpected injury caused by Rachiplusia nu (Guenée) and Crocidosema aporema (Walsingham) in MON 87701 × MON 89788 soybean was detected during the 2020/21 season, and studies confirmed a genetically based alteration in their susceptibility to Cry1Ac. MON 87701 × MON 89788 soybean remains effective against Anticarsia gemmatalis (Hübner), C. includens, Chloridea virescents (Fabricius) and Helicoverpa armigera (Hübner) in Brazil. However, there is evidence of field-evolved resistance to MON 87701 × MON 89788 soybean by the secondary soybean pests R. nu and C. aporema.
Assessment of earthquake-induced landslide inventories and susceptibility maps using slope unit-based logistic regression and geospatial statistics
Authors: Pokharel Badal , Alvioli Massimiliano , Lim Samsung
Published: October 2021

Inventories of seismically induced landslides provide essential information about the extent and severity of ground effects after an earthquake. Rigorous assessment of the completeness of a landslide inventory and the quality of a landslide susceptibility map derived from the inventory is of paramount importance for disaster management applications. Methods and materials applied while preparing inventories influence their quality, but the criteria for generating an inventory are not standardized. This study considered five landslide inventories prepared by different authors after the 2015 Gorkha earthquake, to assess their differences, understand the implications of their use in producing landslide susceptibility maps in conjunction with standard landslide predisposing factors and logistic regression. We adopted three assessment criteria: (1) an error index to identify the mutual mismatches between the inventories; (2) statistical analysis, to study the inconsistency in predisposing factors and performance of susceptibility maps; and (3) geospatial analysis, to assess differences between the inventories and the corresponding susceptibility maps. Results show that substantial discrepancies exist among the mapped landslides. Although there is no distinct variation in the significance of landslide causative factors and the performance of susceptibility maps, a hot spot analysis and cluster/outlier analysis of the maps revealed notable differences in spatial patterns. The percentages of landslide-prone hot spots and clustered areas are directly proportional to the size of the landslide inventory. The proposed geospatial approaches provide a new perspective to the investigators for the quantitative analysis of earthquake-triggered landslide inventories and susceptibility maps.
Magnitude of low birthweight in malaria endemic settings of Nanoro, rural Burkina Faso: a secondary data analysis
Authors: Lingani Moussa , Zango Serge H. , Valéa Innocent , Valia Daniel , Sanou Maïmouna , Samandoulougou Sékou O. , Robert Annie , Tinto Halidou , Dramaix Michèle , Donnen Philippe
Published: October 2021

Low birthweight (LBW) is a worldwide problem that particularly affects developing countries. However, limited information is available on its magnitude in rural area of Burkina Faso. This study aimed to estimate the prevalence of low birthweight and to identify its associated factors in Nanoro health district. A secondary analysis of data collected during a cross-sectional survey was conducted to assess the prevalence of low birthweight in Nanoro health and demographic surveillance system area (HDSS). Maternal characteristics extracted from antenatal care books or by interview, completed by malaria diagnosis were examined through a multi-level logistic regression to estimate odd-ratios of association with low birthweight. Significance level was set at 5%. Of the 291 neonates examined, the prevalence of low birthweight was 12%. After adjustment for socio-demographic, obstetric and malaria prevention variables, being primigravid (OR = 8.84, [95% CI: 3.72–21.01]), or multigravid with history of stillbirth (OR = 5.03, [95% CI: 1.54–16.40]), as well as the lack of long-lasting insecticide treated bed net use by the mother the night preceding the admission for delivery (OR = 2.5, [95% CI: 1.1–5.9]) were significantly associated with neonate low birthweight. The number of antenatal visits however did not confer any direct benefit on birthweight status within this study area. The prevalence of low birthweight was high in the study area and represents an important public health problem in Burkina Faso. In light of these results, a redefinition of the content of the antenatal care package is needed.
2020 COVID-19 lockdown and the impacts on air quality with emphasis on urban, suburban and rural zones
Authors: Slezakova Klara , Pereira Maria Carmo
Published: October 2021

Air quality improvements pollution changes due to COVID-19 restrictions have been reported for many urban developments and large metropolitan areas, but the respective impacts at rural and remote zones are less frequently analysed. This study evaluated air pollution changes across all Portugal (68 stations) considering all urban, suburban and rural zones. PM10, PM2.5, NO2, SO2, ozone was analysed in pre-, during, and post-lockdown period (January–May 2020) and for a comparison also in 2019. NO2 was the most reduced pollutant in 2020, which coincided with decreased traffic. Significant drop (15–71%) of traffic related NO2 was observed specifically during lockdown period, being 55% for the largest and most populated region in country. PM was affected to a lesser degree (with substantial differences found for largely populated areas (Lisbon region ~ 30%; North region, up to 49%); during lockdown traffic-related PM dropped 10–70%. PM10 daily limit was exceeded 50% less in 2020, with 80% of exceedances before lockdown period. SO2 decreased by 35%, due to suspended industrial productions, whereas ozone concentrations slightly (though not significantly) increased (83 vs. 80 µg m–3).
Differential power of placebo across major psychiatric disorders: a preliminary meta-analysis and machine learning study
Authors: Cao Bo , Liu Yang S. , Selvitella Alessandro , Librenza-Garcia Diego , Passos Ives Cavalcante , Sawalha Jeffrey , Ballester Pedro , Chen Jianshan , Dong Shimiao , Wang Fei , Kapczinski Flavio , Dursun Serdar M. , Li Xin-Min , Greiner Russell , Greenshaw Andrew
Published: October 2021

The placebo effect across psychiatric disorders is still not well understood. In the present study, we conducted meta-analyses including meta-regression, and machine learning analyses to investigate whether the power of placebo effect depends on the types of psychiatric disorders. We included 108 clinical trials (32,035 participants) investigating pharmacological intervention effects on major depressive disorder (MDD), bipolar disorder (BD) and schizophrenia (SCZ). We developed measures based on clinical rating scales and Clinical Global Impression scores to compare placebo effects across these disorders. We performed meta-analysis including meta-regression using sample-size weighted bootstrapping techniques, and machine learning analysis to identify the disorder type included in a trial based on the placebo response. Consistently through multiple measures and analyses, we found differential placebo effects across the three disorders, and found lower placebo effect in SCZ compared to mood disorders. The differential placebo effects could also distinguish the condition involved in each trial between SCZ and mood disorders with machine learning. Our study indicates differential placebo effect across MDD, BD, and SCZ, which is important for future neurobiological studies of placebo effects across psychiatric disorders and may lead to potential therapeutic applications of placebo on disorders more responsive to placebo compared to other conditions.
Auto-qPCR; a python-based web app for automated and reproducible analysis of qPCR data
Authors: Maussion Gilles , Thomas Rhalena A. , Demirova Iveta , Gu Gracia , Cai Eddie , Chen Carol X.-Q. , Abdian Narges , Strauss Theodore J. P. , Kelaï Sabah , Nauleau-Javaudin Angela , Beitel Lenore K. , Ramoz Nicolas , Gorwood Philip , Durcan Thomas M.
Published: October 2021

Quantifying changes in DNA and RNA levels is essential in numerous molecular biology protocols. Quantitative real time PCR (qPCR) techniques have evolved to become commonplace, however, data analysis includes many time-consuming and cumbersome steps, which can lead to mistakes and misinterpretation of data. To address these bottlenecks, we have developed an open-source Python software to automate processing of result spreadsheets from qPCR machines, employing calculations usually performed manually. Auto-qPCR is a tool that saves time when computing qPCR data, helping to ensure reproducibility of qPCR experiment analyses. Our web-based app (https://auto-q-pcr.com/) is easy to use and does not require programming knowledge or software installation. Using Auto-qPCR, we provide examples of data treatment, display and statistical analyses for four different data processing modes within one program: (1) DNA quantification to identify genomic deletion or duplication events; (2) assessment of gene expression levels using an absolute model, and relative quantification (3) with or (4) without a reference sample. Our open access Auto-qPCR software saves the time of manual data analysis and provides a more systematic workflow, minimizing the risk of errors. Our program constitutes a new tool that can be incorporated into bioinformatic and molecular biology pipelines in clinical and research labs.