bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluation on Net Energy of Defatted Rice Bran from Different Origins and Processing Technologies Fed to Growing Pigs
Authors: Huang Bingbing , Wang Li , Lyu Zhiqian , Wang Lu , Zang Jianjun , Li Defa , Lai Changhua
Published: April 2021

The study was conducted to determine and compare the net energy (NE) of defatted rice bran (DFRB) from different sources and different processing technology fed to growing pigs using indirect calorimetry. Thirty-six growing barrows (30.7 ± 3.9 kg) were randomly allotted to 1 of 6 diets with 6 replicate pigs per diet. Diets included a corn-soybean meal basal diet and 5 test diets containing 30% DFRB, respectively. These five samples come from 4 different provinces (i.e., Heilongjiang, Jiangsu, Jilin, and Liaoning province within China) and two of them with the same origin but different processing technologies (i.e., extruded or pelleted). During each period, pigs were kept individually in metabolism crates for 21 days, including 14 days to adapt to the diets. On day 15, pigs were transferred to the open-circuit respiration chambers for adaptation, and the next day were ready to determine daily total heat production (HP) and were fed 1 of the 6 diets at 2.3 MJ metabolizable energy (ME)/kg body weight (BW)0.6/day. Total feces and urine were collected for the determination of digestible energy (DE) and ME and daily total HP was measured from day 16 to day 20 and fasted on day 21 for the measurement of fasting heat production (FHP). The NE contents of extruded DFRB from different provinces were within the range of values (8.24 to 10.22 MJ/kg DM). There is a discrepancy of approximately 10.01% in the NE content between the DFRB origins. The NE contents of extruded DFRB and pelleted DFRB from the same province were 8.24 vs. 6.56 MJ/kg DM. Retained energy (RE) and FHP of diets containing extruded DFRB and pelleted DFRB were 1105 vs. 892 kJ/kg BW0.6/day and 746 vs. 726 kJ/kg BW0.6/day respectively, and those in extruded DFRB from different origins were within the range of values (947 to 1105 kJ/kg BW0.6/day and 726 to 755 kJ/kg BW0.6/day, respectively). In conclusion, NE values are affected by origin and processing technology of DFRB.
Dynamic Changes in Fecal Microbial Communities of Neonatal Dairy Calves by Aging and Diarrhea
Authors: Kim Eun-Tae , Lee Sang-Jin , Kim Tae-Yong , Lee Hyo-Gun , Atikur Rahman M. , Gu Bon-Hee , Kim Dong-Hyeon , Park Beom-Young , Son Jun-Kyu , Kim Myung-Hoo
Published: April 2021

Microbiota plays a critical role in the overall growth performance and health status of dairy cows, especially during their early life. Several studies have reported that fecal microbiome of neonatal calves is shifted by various factors such as diarrhea, antibiotic treatment, or environmental changes. Despite the importance of gut microbiome, a lack of knowledge regarding the composition and functions of microbiota impedes the development of new strategies for improving growth performance and disease resistance during the neonatal calf period. In this study, we utilized next-generation sequencing to monitor the time-dependent dynamics of the gut microbiota of dairy calves before weaning (1–8 weeks of age) and further investigated the microbiome changes caused by diarrhea. Metagenomic analysis revealed that continuous changes, including increasing gut microbiome diversity, occurred from 1 to 5 weeks of age. However, the composition and diversity of the fecal microbiome did not change after 6 weeks of age. The most prominent changes in the fecal microbiome composition caused by aging at family level were a decreased abundance of Bacteroidaceae and Enterobacteriaceae and an increased abundance of Prevotellaceae. Phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) analysis indicated that the abundance of microbial genes associated with various metabolic pathways changed with aging. All calves with diarrhea symptoms showed drastic microbiome changes and about a week later returned to the microbiome of pre-diarrheal stage regardless of age. At phylum level, abundance of Bacteroidetes was decreased (p = 0.09) and that of Proteobacteria increased (p = 0.07) during diarrhea. PICRUSt analysis indicated that microbial metabolism-related genes, such as starch and sucrose metabolism, sphingolipid metabolism, alanine aspartate, and glutamate metabolism were significantly altered in diarrheal calves. Together, these results highlight the important implications of gut microbiota in gut metabolism and health status of neonatal dairy calves.
“What Would You Do?”: How Cat Owners Make End-of-Life Decisions and Implications for Veterinary-Client Interactions
Authors: Littlewood Katherine , Beausoleil Ngaio , Stafford Kevin , Stephens Christine
Published: April 2021

Cats are the most common companion animals in New Zealand. Advances in veterinary care means that cats are living longer and there are many older cats. End-of-life decisions about cats are complicated by owner–cat relationships and other psychosocial factors. Our study explored the ways in which end-of-life decisions were being made by owners of older and chronically ill cats in New Zealand and the role of their veterinarian in the process. Qualitative data were gathered via retrospective semi-structured interviews with 14 cat owners using open-ended questions. Transcripts of these interviews were explored for themes using template analysis and nine themes were identified. Four were animal-centered themes: cat behavior change, pain was a bad sign, signs of ageing are not good, and the benefits of having other people see what owners often could not. Five were human-centered themes: veterinarians understanding owners’ relationships with their cat, normalizing death, the need for a good veterinarian to manage end of life, veterinary validation that owners were doing the right thing, and a strong desire to predict the time course and outcome for their cat. End-of-life decision making is complex, and the veterinarian’s role is often poorly defined. Our owners appreciated the expertise and validation that their veterinarian provided but continuity of care was important. Future research aimed at exploring the veterinarian’s perspective during end-of-life decision making for cats would be a valuable addition to the topic.
In Vitro Antibacterial, Anti-Adhesive and Anti-Biofilm Activities of Krameria lappacea (Dombey) Burdet & B.B. Simpson Root Extract against Methicillin-Resistant Staphylococcus aureus Strains
Authors: Genovese Carlo , D’Angeli Floriana , Bellia Francesco , Distefano Alfio , Spampinato Mariarita , Attanasio Francesco , Nicolosi Daria , Di Salvatore Valentina , Tempera Gianna , Lo Furno Debora , Mannino Giuliana , Milardo Fabio , Li Volti Giovanni
Published: April 2021

Methicillin-resistant Staphylococcus aureus (MRSA) represents a serious threat to public health, due to its large variety of pathogenetic mechanisms. Accordingly, the present study aimed to investigate the anti-MRSA activities of Krameria lappacea, a medicinal plant native to South America. Through Ultra-High-Performance Liquid Chromatography coupled with High-Resolution Mass spectrometry, we analyzed the chemical composition of Krameria lappacea root extract (KLRE). The antibacterial activity of KLRE was determined by the broth microdilution method, also including the minimum biofilm inhibitory concentration and minimum biofilm eradication concentration. Besides, we evaluated the effect on adhesion and invasion of human lung carcinoma A549 cell line by MRSA strains. The obtained results revealed an interesting antimicrobial action of this extract, which efficiently inhibit the growth, biofilm formation, adhesion and invasion of MRSA strains. Furthermore, the chemical analysis revealed the presence in the extract of several flavonoid compounds and type-A and type-B proanthocyanidins, which are known for their anti-adhesive effects. Taken together, our findings showed an interesting antimicrobial activity of KLRE, giving an important contribution to the current knowledge on the biological activities of this plant.
Natural and Enantiopure Alkylglycerols as Antibiofilms Against Clinical Bacterial Isolates and Quorum Sensing Inhibitors of Chromobacterium violaceum ATCC 12472
Authors: Chaverra Daza Klauss E. , Silva Gómez Edelberto , Moreno Murillo Bárbara D. , Mayorga Wandurraga Humberto
Published: April 2021

Resistance mechanisms occur in almost all clinical bacterial isolates and represent one of the most worrisome health problems worldwide. Bacteria can form biofilms and communicate through quorum sensing (QS), which allow them to develop resistance against conventional antibiotics. Thus, new therapeutic candidates are sought. We focus on alkylglycerols (AKGs) because of their recently discovered quorum sensing inhibition (QSI) ability and antibiofilm potential. Fifteen natural enantiopure AKGs were tested to determine their effect on the biofilm formation of other clinical bacterial isolates, two reference strains and their QSI was determined using Chromobacterium violaceum ATCC 12472. The highest biofilm inhibition rates (%) and minimum QS inhibitory concentration were determined by a microtiter plate assay and ciprofloxacin was used as the standard antibiotic. At subinhibitory concentrations, each AKG reduced biofilm formation in a concentration-dependent manner against seven bacterial isolates, with values up to 97.2%. Each AKG displayed QSI at different levels of ability without affecting the growth of C. violaceum. AKG (2S)-3-O-(cis-13’-docosenyl)-1,2-propanediol was the best QS inhibitor (20 μM), while (2S)-3-O-(cis-9’-hexadecenyl)-1,2-propanediol was the least effective (795 μM). The results showed for the first time the QSI activity of this natural AKG series and suggest that AKGs could be promising candidates for further studies on preventing antimicrobial resistance.
Quality and Utility of Information Captured by Surveillance Systems Relevant to Antimicrobial Resistance (AMR): A Systematic Review
Authors: Al-Haboubi Mustafa , Glover Rebecca E. , Eastmure Elizabeth , Petticrew Mark , Black Nick , Mays Nicholas
Published: April 2021

Health surveillance systems are considered vital for combatting antimicrobial resistance (AMR); however, the evidence-base on the effectiveness of these systems in providing information that can be used by healthcare professionals, or the acceptability of these systems by users, has not been reviewed. A systematic review was conducted of a number of databases to synthesise the evidence. The review identified 43 studies that met the inclusion criteria, conducted in 18 countries and used 11 attributes in their assessment of surveillance systems. The majority of systems evaluated were for monitoring the incidence of tuberculosis. The studies found that most surveillance systems were underperforming in key attributes that relate to both effectiveness and acceptability. We identified that two features of systems (ease of use and users’ awareness of systems) were associated with greater acceptability and completeness of systems. We recommend prioritising these for the improvement of existing systems, as well as ensuring consistency in the definition of attributes studied, to allow a more consistent approach in evaluations of surveillance systems, and to facilitate the identification of the attributes that have the greatest impact on the utility of data produced.
Association between the blaCTX-M-14-harboring Escherichia coli Isolated from Weasels and Domestic Animals Reared on a University Campus
Authors: Yossapol Montira , Yamamoto Miku , Sugiyama Michiyo , Odoi Justice Opare , Omatsu Tsutomu , Mizutani Tetsuya , Ohya Kenji , Asai Tetsuo
Published: April 2021

Antimicrobial-resistant (AMR) bacteria affect human and animal health worldwide. Here, CTX-M-14-producing Escherichia coli isolates were isolated from Siberian weasels (Mustela sibirica) that were captured on a veterinary campus. To clarify the source of bacteria in the weasels, we examined the domestic animals reared in seven facilities on the campus. Extended-spectrum β-lactamase (ESBL)-producing E. coli were isolated on deoxycholate hydrogen sulfide lactose agar, containing cephalexin (50 μg/mL) or cefotaxime (2 μg/mL), and were characterized with antimicrobial susceptibility testing, pulsed-field gel electrophoresis (PFGE), replicon typing, and β-lactamase typing analyses. Next-generation sequencing of the ESBL-encoding plasmids was also performed. CTX-M-14 producers isolated from both domestic animals and weasels were classified into six clusters with seven PFGE profiles. The PFGE and antimicrobial resistance profiles were characterized by the animal facility. All CTX-M-14 plasmids belonged to the IncI1 type with a similar size (98.9–99.3 kb), except for one plasmid that was 105.5 kb in length. The unweighted pair group method with arithmetic mean (UPGMA) revealed that the CTX-M-14 plasmid in the weasel isolates might have the same origin as the CTX-M-14 plasmid in the domestic animals. Our findings shed further light on the association of antimicrobial resistance between wild and domestic animals.
The Bacterial Genomic Context of Highly Trimethoprim-Resistant DfrB Dihydrofolate Reductases Highlights an Emerging Threat to Public Health
Authors: Lemay-St-Denis Claudèle , Diwan Sarah-Slim , Pelletier Joelle N.
Published: April 2021

Type B dihydrofolate reductase (dfrb) genes were identified following the introduction of trimethoprim in the 1960s. Although they intrinsically confer resistance to trimethoprim (TMP) that is orders of magnitude greater than through other mechanisms, the distribution and prevalence of these short (237 bp) genes is unknown. Indeed, this knowledge has been hampered by systematic biases in search methodologies. Here, we investigate the genomic context of dfrbs to gain information on their current distribution in bacterial genomes. Upon searching publicly available databases, we identified 61 sequences containing dfrbs within an analyzable genomic context. The majority (70%) of those sequences also harbor virulence genes and 97% of the dfrbs are found near a mobile genetic element, representing a potential risk for antibiotic resistance genes. We further identified and confirmed the TMP-resistant phenotype of two new members of the family, dfrb10 and dfrb11. Dfrbs are found both in Betaproteobacteria and Gammaproteobacteria, a majority (59%) being in Pseudomonas aeruginosa. Previously labelled as strictly plasmid-borne, we found 69% of dfrbs in the chromosome of pathogenic bacteria. Our results demonstrate that the intrinsically TMP-resistant dfrbs are a potential emerging threat to public health and justify closer surveillance of these genes.
Adsorption of Phenazines Produced by Pseudomonas aeruginosa Using AST-120 Decreases Pyocyanin-Associated Cytotoxicity
Authors: Hirakawa Hidetada , Takita Ayako , Uchida Motoyuki , Kaneko Yuka , Kakishima Yuto , Tanimoto Koichi , Kamitani Wataru , Tomita Haruyoshi
Published: April 2021

AST-120 (Kremezin) is used to treat progressive chronic kidney disease by adsorbing uremic toxin precursors produced by the gut microbiota, such as indole and phenols. Previously, we found that AST-120 decreased drug tolerance and virulence in Escherichia coli by adsorbing indole. Here, we show that AST-120 adsorbs phenazine compounds, such as pyocyanin, produced by Pseudomonas aeruginosa including multidrug-resistant P. aeruginosa strains, and suppresses pyocyanin-associated toxicity in A-549 (alveolar adenocarcinoma) and Caco-2 (colon adenocarcinoma) cells. Addition of fosfomycin, colistin and amikacin, which are often used to treat P. aeruginosa, inhibited the bacterial growth, regardless of the presence or absence of AST-120. These results suggest a further benefit of AST-120 that supports anti-Pseudomonas chemotherapy in addition to that of E. coli and propose a novel method to treat P. aeruginosa infection.
Resistance and Adaptation of Bacteria to Non-Antibiotic Antibacterial Agents: Physical Stressors, Nanoparticles, and Bacteriophages
Authors: Raza Sada , Matuła Kinga , Karoń Sylwia , Paczesny Jan
Published: April 2021

Antimicrobial resistance is a significant threat to human health worldwide, forcing scientists to explore non-traditional antibacterial agents to support rapid interventions and combat the emergence and spread of drug resistant bacteria. Many new antibiotic-free approaches are being developed while the old ones are being revised, resulting in creating unique solutions that arise at the interface of physics, nanotechnology, and microbiology. Specifically, physical factors (e.g., pressure, temperature, UV light) are increasingly used for industrial sterilization. Nanoparticles (unmodified or in combination with toxic compounds) are also applied to circumvent in vivo drug resistance mechanisms in bacteria. Recently, bacteriophage-based treatments are also gaining momentum due to their high bactericidal activity and specificity. Although the number of novel approaches for tackling the antimicrobial resistance crisis is snowballing, it is still unclear if any proposed solutions would provide a long-term remedy. This review aims to provide a detailed overview of how bacteria acquire resistance against these non-antibiotic factors. We also discuss innate bacterial defense systems and how bacteriophages have evolved to tackle them.
Ascorbate Peroxidase Neofunctionalization at the Origin of APX-R and APX-L: Evidence from Basal Archaeplastida
Authors: Lazzarotto Fernanda , Menguer Paloma Koprovski , Del-Bem Luiz-Eduardo , Zámocký Marcel , Margis-Pinheiro Márcia
Published: April 2021

Ascorbate peroxidases (APX) are class I members of the Peroxidase-Catalase superfamily, a large group of evolutionarily related but rather divergent enzymes. Through mining in public databases, unusual subsets of APX homologs were identified, disclosing the existence of two yet uncharacterized families of peroxidases named ascorbate peroxidase-related (APX-R) and ascorbate peroxidase-like (APX-L). As APX, APX-R harbor all catalytic residues required for peroxidatic activity. Nevertheless, proteins of this family do not contain residues known to be critical for ascorbate binding and therefore cannot use it as an electron donor. On the other hand, APX-L proteins not only lack ascorbate-binding residues, but also every other residue known to be essential for peroxidase activity. Through a molecular phylogenetic analysis performed with sequences derived from basal Archaeplastida, the present study discloses the existence of hybrid proteins, which combine features of these three families. The results here presented show that the prevalence of hybrid proteins varies among distinct groups of organisms, accounting for up to 33% of total APX homologs in species of green algae. The analysis of this heterogeneous group of proteins sheds light on the origin of APX-R and APX-L and suggests the occurrence of a process characterized by the progressive deterioration of ascorbate-binding and catalytic sites towards neofunctionalization.
Trapped Proton Fluxes Estimation Inside the South Atlantic Anomaly Using the NASA AE9/AP9/SPM Radiation Models along the China Seismo-Electromagnetic Satellite Orbit
Authors: Martucci Matteo , Sparvoli Roberta , Bartocci Simona , Battiston Roberto , Burger William Jerome , Campana Donatella , Carfora Luca , Castellini Guido , Conti Livio , Contin Andrea , De Donato Cinzia , De Santis Cristian , Follega Francesco Maria , Iuppa Roberto , Lazzizzera Ignazio , Marcelli Nadir , Masciantonio Giuseppe , Mergé Matteo , Oliva Alberto , Osteria Giuseppe , Palma Francesco , Palmonari Federico , Panico Beatrice , Parmentier Alexandra , Perfetto Francesco , Picozza Piergiorgio , Piersanti Mirko , Pozzato Michele , Ricci Ester , Ricci Marco , Ricciarini Sergio Bruno , Sahnoun Zouleikha , Scotti Valentina , Sotgiu Alessandro , Vitale Vincenzo , Zoffoli Simona , Zuccon Paolo
Published: April 2021

The radiation belts in the Earth’s magnetosphere pose a hazard to satellite systems and spacecraft missions (both manned and unmanned), heavily affecting payload design and resources, thus resulting in an impact on the overall mission performance and final costs. The NASA AE9/AP9/SPM radiation models for energetic electrons, protons, and plasma provide useful information on the near-Earth environment, but they are still incomplete as to some features and, for some energy ranges, their predictions are not based on a statistically sufficient sample of direct measurements. Therefore, it is of the upmost importance to provide new data and direct measurements to improve their output. In this work, the AP9 model is applied to the China Seismo-Electromagnetic Satellite (CSES-01) orbit to estimate the flux of energetic protons over the South Atlantic Anomaly during a short testing period of one day, 1 January 2021. Moreover, a preliminary comparison with proton data obtained from the High-Energy Particle Detector (HEPD) on board CSES-01 is carried out. This estimation will serve as the starting ground for a forthcoming complete data analysis, enabling extensive testing and validation of current theoretical and empirical models.
An Empirical Study of Drivers, Barriers, and Cost Efficiency of Information Systems in Greek Industry
Authors: Kapetanopoulou Paraskevi , Kouroutzi Antigoni
Published: April 2021

The business environment is characterized by high complexity and competitiveness. This is the reason why information is considered to be the most valuable source companies have available. From the social point of view, information technology (IT) has a positive effect on business performance, creates strong and transparent relations with enterprise’s clients, and increases competition. The purpose of this study is to assess the current state of information systems (ISs) adoption by Greek industry. The main issues that are explored through this survey in regards to IS adoption is the extent, the most important specific drivers and barriers, and the profitability due to IS adoption. The study also investigates whether motivators or inhibitors are affected by the factor of profitability due to IS adoption. The survey that was conducted is questionnaire-based and the responses were analyzed statistically using several statistical tools. The results suggest that the majority of the industries in Greece have adopted IS to conduct their business. Reducing errors that occur during their business activities seems to be their main motivation for IS adoption. On the other hand, they are hesitant to invest in an IS because of its cost.
Application of Wood Composites
Authors: Krišťák Ľuboš , Réh Roman
Published: April 2021

Wood composites are the key material for a number of structural and non-structural applications for interior and exterior purposes, such as furniture, construction, floorings, windows and doors, etc [...]
Analysis of Chemical Biomarkers and Contaminants in Food
Authors: Dal Bello Federica , Medana Claudio
Published: April 2021

The quality of food has become of great importance and relevance in recent years, not only for scientific communities but also for ordinary people [...]
Development of a Component-Level Hydrogen Transport Model with OpenFOAM and Application to Tritium Transport Inside a DEMO HCPB Breeder
Authors: Pasler Volker , Arbeiter Frederik , Klein Christine , Klimenko Dmitry , Schlindwein Georg , von der Weth Axel
Published: April 2021

This work continues the development of a numerical model to simulate transient tritium transport on the breeder zone (BZ) level for the EU helium-cooled pebble bed (HCPB) concept for DEMO. The basis of the model is the open-source field operation and manipulation framework, OpenFOAM. The key output quantities of the model are the tritium concentration in the purge gas and in the coolant and the tritium inventory inside the BZ structure. New model features are briefly summarized. As a first relevant application a simulation of tritium transport for a single pin out of the KIT HCPB design for DEMO is presented. A variety of scenarios investigates the impact of the permeation regime (diffusion-limited vs. surface-limited), of an additional hydrogen content of 300 Pa H2 in the purge gas, of the released species (HT vs. T2), and of the choice of species-specific rate constants (recombination constant of HT set twice as for H2 and T2). The results indicate that the released species plays a minor role for permeation. Both permeation and inventory show a considerable dependence on a possible hydrogen addition in the purge gas. An enhanced HT recombination constant reduces steel T inventories and, in the diffusion-limited case, also permeation significantly. Scenarios with 80 bar vs. 2 bar purge gas pressure indicate that purge gas volumetric flow is decisive for permeation.
Digital Modelling of Underground Volumes, Including the Visualization of Confidence Levels for the Positioning of Subsurface Objects
Authors: Adouane Kamel , Boujon Fabian , Domer Bernd
Published: April 2021

This paper addresses the issue of offering a consistent 3D visual rendering of subsurface objects when databases face non-completion. Digital modelling of subsurface objects, like utility lines, underground buildings or tree roots, is a difficult task. Data available are incomplete and not precise. The in situ acquisition of existing objects to increase data quality is complex and, therefore, costly. In this paper, a methodology to obtain missing spatial and geometrical data through field or empirical means is proposed. In addition, confidence levels are assigned to existing and derived spatial and geometrical attributes. They are consolidated on a class level and visualized through a bounding shape, called secondary object.
Classification with Fuzzification Optimization Combining Fuzzy Information Systems and Type-2 Fuzzy Inference
Authors: Tabakov Martin , Chlopowiec Adrian , Chlopowiec Adam , Dlubak Adam
Published: April 2021

In this research, we introduce a classification procedure based on rule induction and fuzzy reasoning. The classifier generalizes attribute information to handle uncertainty, which often occurs in real data. To induce fuzzy rules, we define the corresponding fuzzy information system. A transformation of the derived rules into interval type-2 fuzzy rules is provided as well. The fuzzification applied is optimized with respect to the footprint of uncertainty of the corresponding type-2 fuzzy sets. The classification process is related to a Mamdani type fuzzy inference. The method proposed was evaluated by the F-score measure on benchmark data.
4-Hexylresorcinol Inhibits Class I Histone Deacetylases in Human Umbilical Cord Endothelial Cells
Authors: Kim Jwa-Young , Kweon Hae-Yong , Kim Dae-Won , Choi Je-Yong , Kim Seong-Gon
Published: April 2021

Histone deacetylases (HDACs) are key enzymes for post-translational modification and influence on various cellular activities. Thus, HDACs are associated with many diseases and their inhibitors have clinical significance. Here, 4-Hexylresorcinol (4HR) was studied as an inhibitor for class I HDACs using the HDAC inhibitor (HDACi) Trichostatin-A as a positive control. The 4HR was administered 1–100 μM to human umbilical endothelial cells (HUVECs) and the HDAC expression and activity were examined. The 4HR decreased the expression level of HDAC1, 3, 4, and 5 in a time and dose-dependent manner. The 4HR also increased acetylated lysine and decreased HDAC activity significantly (p < 0.05). Collectively, 4HR was a new class I HDAC inhibitor that reduced the expression and activity of HDAC in HUVECs.
Evaluation of Structural Performance of Post-Installed Anchors Embedded in Cracked Concrete in Power Plant Facilities
Authors: Lee Sangmoon , Jung Wooyoung
Published: April 2021

In this study, a field survey was conducted on the fixed anchorages of the operation and power generation facilities installed in domestic power plants. A static/dynamic performance evaluation was conducted to present safety evaluation guidelines that meet the domestic seismic performance requirements. Seismic performance tests were performed on the post-installed set anchors M10 and M12, which are mainly used for anchorages in accordance with the US and European seismic performance standards. The dynamic shear test results showed that the M12 anchor met the seismic performance verification criterion, whereas the M10 anchor did not because its dynamic performance was reduced, owing to the cyclic loading. In the results of the dynamic pull-out test, M12 also met the seismic performance verification criterion, whereas M10 was safe only in a non-cracked state. In summary, the seismic performance of M12 in both cracks and non-cracks was satisfied, but, in the case of M10, the results were not satisfied in cracks. This was an experimental study; it will be necessary to conduct additional analytical research in the future to verify the reliability and parameters of the experiment.
Can Body Fat Percentage, Body Mass Index, and Specific Field Tests Explain Throwing Ball Velocity in Team Handball Players?
Authors: Hermassi Souhail , Hayes Lawrence D. , Schwesig René
Published: April 2021

This study examined the physical fitness, anthropometry, and throwing velocity of normal weight and overweight male team handball players. Twenty-five players from the Qatar handball first professional league participated. The anthropometry and physical performance—yo-yo intermittent recovery test, jumping ability (squat and countermovement jumps (SJ and CMJ)), T-half test for change-of-direction (COD) ability, sprint tests (15 and 30 m), and 3 kg medicine ball overhead throw—was measured. The handball-throwing velocity was measured using a three-step running shot and a jump shot. Only the CMJ (p = 0.016, ηp2 = 0.227) was different between the normal and overweight subjects. Two-step linear regression analysis using body height and body mass (step 1) and percentage body fat (%BF) (step 2) predictors showed an increase in the explained variance by adding %BF. The largest r2 change was calculated for COD (0.53). The predictive ability was observed for CMJ (step 1: r2 = 0.18, step 2: r2 = 0.22) and SJ (step 1: r2 = 0.05, step 2: r2 = 0.09). With the exception of the sprint parameters (β-coefficient 15 m sprint: −0.07; β-coefficient 30 m sprint: −0.06) and COD (β-coefficient: −0.09), a lower %BF was associated with greater performance in all parameters. %BF seems to be important for predicting sprint and agility performance.
IoT and Deep Learning Based Approach for Rapid Screening and Face Mask Detection for Infection Spread Control of COVID-19
Authors: Hussain Shabir , Yu Yang , Ayoub Muhammad , Khan Akmal , Rehman Rukhshanda , Wahid Junaid Abdul , Hou Weiyan
Published: April 2021

The spread of COVID-19 has been taken on pandemic magnitudes and has already spread over 200 countries in a few months. In this time of emergency of COVID-19, especially when there is still a need to follow the precautions and developed vaccines are not available to all the developing countries in the first phase of vaccine distribution, the virus is spreading rapidly through direct and indirect contacts. The World Health Organization (WHO) provides the standard recommendations on preventing the spread of COVID-19 and the importance of face masks for protection from the virus. The excessive use of manual disinfection systems has also become a source of infection. That is why this research aims to design and develop a low-cost, rapid, scalable, and effective virus spread control and screening system to minimize the chances and risk of spread of COVID-19. We proposed an IoT-based Smart Screening and Disinfection Walkthrough Gate (SSDWG) for all public places entrance. The SSDWG is designed to do rapid screening, including temperature measuring using a contact-free sensor and storing the record of the suspected individual for further control and monitoring. Our proposed IoT-based screening system also implemented real-time deep learning models for face mask detection and classification. This module classified individuals who wear the face mask properly, improperly, and without a face mask using VGG-16, MobileNetV2, Inception v3, ResNet-50, and CNN using a transfer learning approach. We achieved the highest accuracy of 99.81% while using VGG-16 and the second highest accuracy of 99.6% using MobileNetV2 in the mask detection and classification module. We also implemented classification to classify the types of face masks worn by the individuals, either N-95 or surgical masks. We also compared the results of our proposed system with state-of-the-art methods, and we highly suggested that our system could be used to prevent the spread of local transmission and reduce the chances of human carriers of COVID-19.
Lower Limb Kinematics in Individuals with Hip Osteoarthritis during Gait: A Focus on Adaptative Strategies and Interlimb Symmetry
Authors: Porta Micaela , Pau Massimiliano , Leban Bruno , Deidda Michela , Sorrentino Marco , Arippa Federico , Marongiu Giuseppe
Published: April 2021

Among the functional limitations associated with hip osteoarthritis (OA), the alteration of gait capabilities represents one of the most invalidating as it may seriously compromise the quality of life of the affected individual. The use of quantitative techniques for human movement analysis has been found valuable in providing accurate and objective measures of kinematics and kinetics of gait in individuals with hip OA, but few studies have reported in-depth analyses of lower limb joint kinematics during gait and, in particular, there is a scarcity of data on interlimb symmetry. Such aspects were investigated in the present study which tested 11 individuals with hip OA (mean age 68.3 years) and 11 healthy controls age- and sex-matched, using 3D computerized gait analysis to perform point-by-point comparisons of the joint angle trends of hip, knee, and ankle. Angle-angle diagrams (cyclograms) were also built to compute several parameters (i.e., cyclogram area and orientation and Trend Symmetry) from which to assess the degree of interlimb symmetry. The results show that individuals with hip OA exhibit peculiar gait patterns characterized by severe modifications of the physiologic trend at hip level even in the unaffected limb (especially during the stance phase), as well as minor (although significant) alterations at knee and ankle level. The symmetry analysis also revealed that the effect of the disease in terms of interlimb coordination is present at knee joint as well as hip, while the ankle joint appears relatively preserved from specific negative effects from this point of view. The availability of data on such kinematic adaptations may be useful in supporting the design of specific rehabilitative strategies during both preoperative and postoperative periods.
Tachyporinae Revisited: Phylogeny, Evolution, and Higher Classification Based on Morphology, with Recognition of a New Rove Beetle Subfamily (Coleoptera: Staphylinidae)
Authors: Yamamoto Shûhei
Published: April 2021

Tachyporinae are one of the most phylogenetically problematic subfamilies in the mega-diverse rove beetle family Staphylinidae. Despite its high diversity and abundance in forest micro-environments, with over 1600 species worldwide, several previous studies had refuted the monophyly of this subfamily and its largest tribe, Tachyporini. Based on the results of morphology-based phylogenetic analyses and direct examination of specimens encompassing two extinct and all forty extant genera, a new suprageneric classification of Tachyporinae is proposed here, with the removal of the tribe Mycetoporini into a newly recognized subfamily Mycetoporinae stat. nov. Four tribes with two subtribes are arranged within Tachyporinae sensu nov.: Tachyporini sensu nov. (Tachyporina stat. nov., sensu nov. and Euconosomatina stat. rev., sensu nov.), Vatesini sensu nov., Deropini, and Tachinusini stat. rev., sensu nov. (= Megarthropsini syn. nov.). Urolitus syn. nov. is placed as a junior synonym of Sepedophilus. Additionally, Palporus stat. nov. is raised to a distinct genus from a subgenus of Tachyporus sensu. nov., and †Mesotachyporus syn. nov. is synonymized with the latter. Mycetoporine Bobitobus stat. rev. is resurrected from synonymy with Lordithon sensu nov., and considered as a valid genus. My revised classification provides a novel framework for taxonomic inventories and ecological studies of these groups.
Inflammatory Markers in Cancer Immunotherapy
Authors: Ravindranathan Deepak , Master Viraj A. , Bilen Mehmet Asim
Published: April 2021

Chronic inflammation is considered a major risk factor for cancer formation. Inflammation within the tumor environment plays a role in its response to therapy, growth, and prognosis. Cancer associated inflammation is known to occur in the tumor microenvironment and in the systemic circulation, and is correlated with disease progression and prognosis in many cancers. Blood cells such as neutrophils, lymphocytes, platelets, and circulating proteins such as C-reactive protein, and interleukins, such as IL-6, have been associated with inflammatory responses, which contribute to tumorigenesis. Cancer has found ways to evade the immune response; a pathway that can attenuate the innate immune response is via blocking immune checkpoints. Development of monoclonal antibodies against inhibitory immune checkpoints such as cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) and programmed cell death protein 1 (PD-1) have given rise to immunotherapy, which has shown remarkable responses in anti-tumor activity resulting in several U.S. Federal and Drug Administration (FDA)-approved checkpoint inhibitors. Various inflammatory markers and their prognostic and predictive implications in malignancies treated with immunotherapy will be discussed in this review.
Phosphorylation, Mg-ADP, and Inhibitors Differentially Shape the Conformational Dynamics of the A-Loop of Aurora-A
Authors: Musavizadeh Zahra , Grottesi Alessandro , Guarguaglini Giulia , Paiardini Alessandro
Published: April 2021

The conformational state of the activation loop (A-loop) is pivotal for the activity of most protein kinases. Hence, the characterization of the conformational dynamics of the A-loop is important to increase our understanding of the molecular processes related to diseases and to support the discovery of small molecule kinase inhibitors. Here, we carry out a combination of molecular dynamics (MD) and essential dynamics (ED) analyses to fully map the effects of phosphorylation, ADP, and conformation disrupting (CD) inhibitors (i.e., CD532 and MLN8054) on the dynamics of the A-loop of Aurora-A. MD revealed that the stability of the A-loop in an open conformation is enhanced by single phospho-Thr-288, while paradoxically, the presence of a second phosphorylation at Thr-287 decreases such stability and renders the A-loop more fluctuant in time and space. Moreover, we found that this post-translational modification has a significant effect on the direction of the A-loop motions. ED analysis suggests that the presence of the phosphate moiety induces the dynamics of Aurora-A to sample two distinct energy minima, instead of a single large minimum, as in unphosphorylated Aurora-A states. This observation indicates that the conformational distributions of Aurora-A with both single and double phospho-threonine modifications are remarkably different from the unphosphorylated state. In the closed states, binding of CD532 and MLN8054 inhibitors has the effect of increasing the distance of the N- and C-lobes of the kinase domain of Aurora-A, and the angle analysis between those two lobes during MD simulations showed that the N- and C-lobes are kept more open in presence of CD532, compared to MLN8054. As the A-loop is a common feature of Aurora protein kinases, our studies provide a general description of the conformational dynamics of this structure upon phosphorylation and different ligands binding.
Phaseolin Attenuates Lipopolysaccharide-Induced Inflammation in RAW 264.7 Cells and Zebrafish
Authors: Hwang Su-Jung , Song Ye-Seul , Lee Hyo-Jong
Published: April 2021

Kushen (Radix Sophorae flavescentis) is used to treat ulcerative colitis, tumors, and pruritus. Recently, phaseolin, formononetin, matrine, luteolin, and quercetin, through a network pharmacology approach, were tentatively identified as five bioactive constituents responsible for the anti-inflammatory effects of S. flavescentis. However, the role of phaseolin (one of the primary components of S. flavescentis) in the direct regulation of inflammation and inflammatory processes is not well known. In this study, the beneficial role of phaseolin against inflammation was explored in lipopolysaccharide (LPS)-induced inflammation models of RAW 264.7 macrophages and zebrafish larvae. Phaseolin inhibited LPS-mediated production of nitric oxide (NO) and the expression of inducible nitric oxide synthase (iNOS), without affecting cell viability. In addition, phaseolin suppressed pro-inflammatory mediators such as cyclooxygenase 2 (COX-2), interleukin-1β (IL-1β), tumor necrosis factor α (TNF-α), monocyte chemoattractant protein-1 (MCP-1), and interleukin-6 (IL-6) in a dose-dependent manner. Furthermore, phaseolin reduced matrix metalloproteinase (MMP) activity as well as macrophage adhesion in vitro and the recruitment of leukocytes in vivo by downregulating Ninjurin 1 (Ninj1), an adhesion molecule. Finally, phaseolin inhibited the nuclear translocation of nuclear factor-kappa B (NF-κB). In view of the above, our results suggest that phaseolin could be a potential therapeutic candidate for the management of inflammation.
Defining Hypoperfusion in Chronic Aphasia: An Individualized Thresholding Approach
Authors: Abbott Noelle T. , Baker Carolyn J. , Chen Conan , Liu Thomas T. , Love Tracy E.
Published: April 2021

Within the aphasia literature, it is common to link location of lesioned brain tissue to specific patterns of language impairment. This has provided valuable insight into the relationship between brain structure and function, but it does not capture important underlying alterations in function of regions that remain structurally intact. Research has demonstrated that in the chronic stage of aphasia, variable patterns of reduced cerebral blood flow (CBF; hypoperfusion) in structurally intact regions of the brain contribute to persisting language impairments. However, one consistent issue in this literature is a lack of clear consensus on how to define hypoperfusion, which may lead to over- or underestimation of tissue functionality. In the current study, we conducted an exploratory analysis in six individuals with chronic aphasia (>1 year post-onset) using perfusion imaging to (1) suggest a new, individualized metric for defining hypoperfusion; (2) identify the extent of hypoperfused tissue in perilesional bands; and (3) explore the relationship between hypoperfusion and language impairment. Results indicated that our individualized metric for defining hypoperfusion provided greater precision when identifying functionally impaired tissue and its effects on language function in chronic aphasia. These results have important implications for intervention approaches that target intact (or impaired) brain tissue.
Cognition across the Lifespan: Investigating Age, Sex, and Other Sociodemographic Influences
Authors: Nichols Emily S. , Wild Conor J. , Owen Adrian M. , Soddu Andrea
Published: April 2021

Maintaining cognitive health across the lifespan has been the focus of a multi-billion-dollar industry. In order to guide treatment and interventions, a clear understanding of the way that proficiency in different cognitive domains develops and declines in both sexes across the lifespan is necessary. Additionally, there are sex differences in a range of other factors, including psychiatric illnesses such as anxiety, depression, and substance use, that are also known to affect cognition, although the scale of this interaction is unknown. Our objective was to assess differences in cognitive function across the lifespan in men and women in a large, representative sample. Leveraging online cognitive testing, a sample of 9451 men and 9451 women ranging in age from 12 to 69 (M = 28.21) matched on socio-demographic factors were studied. Segmented regression was used to model three cognitive domains—working memory, verbal abilities, and reasoning. Sex differences in all three domains were minimal; however, after broadening the sample in terms of socio-demographic factors, sex differences appeared. These results suggest that cognition across the lifespan differs for men and women, but is greatly influenced by environmental factors. We discuss these findings within a framework that describes sex differences in cognition as likely guided by a complex interplay between biology and environment.
Distinct Cerebrovascular Reactivity Patterns for Brain Radiation Necrosis
Authors: Muscas Giovanni , van Niftrik Christiaan Hendrik Bas , Sebök Martina , Della Puppa Alessandro , Seystahl Katharina , Andratschke Nicolaus , Brown Michelle , Weller Michael , Regli Luca , Piccirelli Marco , Fierstra Jorn
Published: April 2021

Background: Current imaging-based discrimination between radiation necrosis versus recurrent glioblastoma contrast-enhancing lesions remains imprecise but is paramount for prognostic and therapeutic evaluation. We examined whether patients with radiation necrosis exhibit distinct patterns of blood oxygenation-level dependent fMRI cerebrovascular reactivity (BOLD-CVR) as the first step to better distinguishing patients with radiation necrosis from recurrent glioblastoma compared with patients with newly diagnosed glioblastoma before surgery and radiotherapy. Methods: Eight consecutive patients with primary and secondary brain tumors and a multidisciplinary clinical and radiological diagnosis of radiation necrosis, and fourteen patients with a first diagnosis of glioblastoma underwent BOLD-CVR mapping. For all these patients, the contrast-enhancing lesion was derived from high-resolution T1-weighted MRI and rendered the volume-of-interest (VOI). From this primary VOI, additional 3 mm concentric expanding VOIs up to 30 mm were created for a detailed perilesional BOLD-CVR tissue analysis between the two groups. Receiver operating characteristic curves assessed the discriminative properties of BOLD-CVR for both groups. Results: Mean intralesional BOLD-CVR values were markedly lower in radiation necrosis than in glioblastoma contrast-enhancing lesions (0.001 ± 0.06 vs. 0.057 ± 0.05; p = 0.04). Perilesionally, a characteristic BOLD-CVR pattern was observed for radiation necrosis and glioblastoma patients, with an improvement of BOLD-CVR values in the radiation necrosis group and persisting lower perilesional BOLD-CVR values in glioblastoma patients. The ROC analysis discriminated against both groups when these two parameters were analyzed together (area under the curve: 0.85, 95% CI: 0.65–1.00). Conclusions: In this preliminary analysis, distinctive intralesional and perilesional BOLD-cerebrovascular reactivity patterns are found for radiation necrosis.