bluuebunny/crossref_metadata_2025 huggingface dataset:
Manic and Depressive Symptoms in Children Diagnosed with Noonan Syndrome
Authors: Alfieri Paolo , Cumbo Francesca , Serra Giulia , Trasolini Monia , Frattini Camilla , Scibelli Francesco , Licchelli Serena , Cirillo Flavia , Caciolo Cristina , Casini Maria Pia , D’Amico Adele , Tartaglia Marco , Digilio Maria Cristina , Capolino Rossella , Vicari Stefano
Published: February 2021

Noonan syndrome (NS) is a dominant clinically variable and genetically heterogeneous developmental disorder caused by germ-line mutations encoding components of the Ras–MAPK signaling pathway. A few studies have investigated psychopathological features occurring in individuals with NS, although they were poorly analyzed. The aim of the present work is to investigate the psychopathological features in children and adolescents with NS focusing on depressive and hypo-manic symptoms. Thirty-seven subjects with molecularly confirmed diagnosis were systematically evaluated through a psychopathological assessment. In addition, an evaluation of the cognitive level was performed. Our analyses showed a high recurrence of attention deficit and hyperactivity disorder symptoms, emotional dysregulation, irritability, and anxiety symptomatology. The mean cognitive level was on the average. The present study provides new relevant information on psychopathological features in individuals with NS. The implications for clinicians are discussed including the monitoring of mood disorders in a clinical evolution.
Androgen Affects the Inhibitory Avoidance Memory by Primarily Acting on Androgen Receptor in the Brain in Adolescent Male Rats
Authors: Islam Md Nabiul , Sakimoto Yuya , Jahan Mir Rubayet , Miyasato Emi , Tarif Abu Md Mamun , Nozaki Kanako , Masumoto Koh-hei , Yanai Akie , Mitsushima Dai , Shinoda Koh
Published: February 2021

Adolescence is the critical postnatal stage for the action of androgen in multiple brain regions. Androgens can regulate the learning/memory functions in the brain. It is known that the inhibitory avoidance test can evaluate emotional memory and is believed to be dependent largely on the amygdala and hippocampus. However, the effects of androgen on inhibitory avoidance memory have never been reported in adolescent male rats. In the present study, the effects of androgen on inhibitory avoidance memory and on androgen receptor (AR)-immunoreactivity in the amygdala and hippocampus were studied using behavioral analysis, Western blotting and immunohistochemistry in sham-operated, orchiectomized, orchiectomized + testosterone or orchiectomized + dihydrotestosterone-administered male adolescent rats. Orchiectomized rats showed significantly reduced time spent in the illuminated box after 30 min (test 1) or 24 h (test 2) of electrical foot-shock (training) and reduced AR-immunoreactivity in amygdala/hippocampal cornu Ammonis (CA1) in comparison to those in sham-operated rats. Treatment of orchiectomized rats with either non-aromatizable dihydrotestosterone or aromatizable testosterone were successfully reinstated these effects. Application of flutamide (AR-antagonist) in intact adolescent rats exhibited identical changes to those in orchiectomized rats. These suggest that androgens enhance the inhibitory avoidance memory plausibly by binding with AR in the amygdala and hippocampus.
A Framework for Instantaneous Driver Drowsiness Detection Based on Improved HOG Features and Naïve Bayesian Classification
Authors: Bakheet Samy , Al-Hamadi Ayoub
Published: February 2021

Due to their high distinctiveness, robustness to illumination and simple computation, Histogram of Oriented Gradient (HOG) features have attracted much attention and achieved remarkable success in many computer vision tasks. In this paper, an innovative framework for driver drowsiness detection is proposed, where an adaptive descriptor that possesses the virtue of distinctiveness, robustness and compactness is formed from an improved version of HOG features based on binarized histograms of shifted orientations. The final HOG descriptor generated from binarized HOG features is fed to the trained Naïve Bayes (NB) classifier to make the final driver drowsiness determination. Experimental results on the publicly available NTHU-DDD dataset verify that the proposed framework has the potential to be a strong contender for several state-of-the-art baselines, by achieving a competitive detection accuracy of 85.62%, without loss of efficiency or stability.
Structural Performance of Reinforced Concrete Beams Incorporating Cathode-Ray Tube (CRT) Glass Waste
Authors: Bawab Jad , Khatib Jamal , Jahami Ali , Elkordi Adel , Ghorbel Elhem
Published: February 2021

The performance of reinforced concrete beams in the presence of cathode-ray tube (CRT) glass waste is examined. Four concrete mixes containing 0%, 10%, 20%, and 30% CRT glass waste as partial replacement of sand were prepared. The compressive and flexural strength as well as the modulus of elasticity of concrete were determined. Reinforced concrete beams with varying amounts of CRT glass were prepared and the three-point bending test was conducted. The load-deflection curve as well as the strain distribution along the depth of the beams were determined. Concrete containing CRT glass showed an increase in compressive strength, flexural strength, and modulus of elasticity especially at 10% replacement level. The load carrying capacity of reinforced concrete beam is higher when 10% of sand is replaced with CRT glass compared to the control beam and the beams with 20% and 30% CRT glass substitution. The failure mode of the reinforced concrete beams is flexural failure, and the failure pattern is similar for all beams. Strain distribution showed a better ductility at control beam where the deflection was higher than the other beams at the same load. Numerical analysis was conducted, and comparison was made with the experimental results. The comparison showed the accuracy of the software used, where the results of maximum load capacity and maximum deflection were very similar, and the difference did not exceed 5%. In addition, the tensile damage generated by the numerical analysis was very similar to that obtained by the experimental study.
Prognostic Value of Apoptosis-Inducing Factor (AIF) in Germ Cell Tumors
Authors: Letkovska Katarina , Babal Pavel , Cierna Zuzana , Schmidtova Silvia , Liskova Veronika , Kalavska Katarína , Miskovska Vera , Horak Samuel , Rejlekova Katarina , Chovanec Michal , Mardiak Jozef , Janega Pavel , Mego Michal
Published: February 2021

Apoptosis is a strictly regulated process essential for preservation of tissue homeostasis. This study aimed to evaluate expression of apoptosis inducing factor (AIF) in testicular germ cell tumors (GCTs) and to correlate expression patterns with clinicopathological variables. Formalin-fixed and paraffin-embedded specimens of non-neoplastic testicular tissue and GCTs obtained from 216 patients were included in the study. AIF expression was detected by immunohistochemistry, scored by the multiplicative quickscore method (QS). Normal testicular tissue exhibits higher cytoplasmic granular expression of AIF compared to GCTs (mean QS = 12.77 vs. 4.80, p < 0.0001). Among invasive GCTs, mean QS was the highest in embryonal carcinoma, yolk sac tumor and seminoma, lower in teratoma and the lowest in choriocarcinoma. No nuclear translocation of AIF was observed. Nonpulmonary visceral metastases were associated with lower AIF expression. Metastatic GCTs patients with high AIF expression had better overall survival compared to patients with low AIF expression (HR = 0.26, 95% CI 0.11–0.62, p = 0.048). We observed significantly lower AIF expression in GCTs compared to normal testicular tissue, which is an uncommon finding in malignant tumors. AIF downregulation might represent one of the mechanisms of inhibition of apoptosis and promotion of cell survival in GCTs.
Infant Acute Myeloid Leukemia: A Unique Clinical and Biological Entity
Authors: Calvo Charlotte , Fenneteau Odile , Leverger Guy , Petit Arnaud , Baruchel André , Méchinaud Françoise
Published: February 2021

Infant acute myeloid leukemia (AML) is a rare subgroup of AML of children <2 years of age. It is as frequent as infant acute lymphoblastic leukemia (ALL) but not clearly distinguished by study groups. However, infant AML demonstrates peculiar clinical and biological characteristics, and its prognosis differs from AML in older children. Acute megakaryoblastic leukemia (AMKL) is very frequent in this age group and has raised growing interest. Thus, AMKL is a dominant topic in this review. Recent genomic sequencing has contributed to our understanding of infant AML. These data demonstrated striking features of infant AML: fusion genes are able to induce AML transformation without additional cooperation, and unlike AML in older age groups there is a paucity of associated mutations. Mice modeling of these fusions showed the essential role of ontogeny in the infant leukemia phenotype compared to older children and adults. Understanding leukemogenesis may help in developing new targeted treatments to improve outcomes that are often very poor in this age group. A specific diagnostic and therapeutic approach for this age group should be investigated.
Prognostic Significance of CD4+ and CD8+ Tumor-Infiltrating Lymphocytes in Head and Neck Squamous Cell Carcinoma: A Meta-Analysis
Authors: Borsetto Daniele , Tomasoni Michele , Payne Karl , Polesel Jerry , Deganello Alberto , Bossi Paolo , Tysome James R. , Masterson Liam , Tirelli Giancarlo , Tofanelli Margherita , Boscolo-Rizzo Paolo
Published: February 2021

Objective: It has been suggested that the presence of tumor-infiltrating lymphocytes (TILs) in the tumor microenvironment is associated with a better prognosis in different types of cancer. In this systematic review and meta-analysis, we investigated the prognostic role of CD4+ and CD8+ TILs in head and neck squamous cell carcinoma (HNSCC). Methods: PubMed, Cochrane, Embase, Scopus, and Web of Science were searched up to September 2020. This study was conducted following the preferred reporting items for systematic reviews and meta-analyses (PRISMA) checklist. Risk ratios from individual studies were displayed in forest plots and the pooled hazard ratios (HR) of death and corresponding confidence intervals (CI) were calculated according to random-effects models. Risk of bias of the included studies was assessed through the Newcastle–Ottawa scale. Results: 28 studies met the inclusion criteria. Studies conducted on HNSCC subsites combined reported a significant reduction in the risk of death for both high CD4+ (HR: 0.77; 95% CI: 0.65–0.93) and high CD8+ TILs (HR: 0.64; 95% CI: 0.47–0.88). High CD4+ TILs were associated with significantly better overall survival among oropharyngeal HNSCC (HR: 0.52; 95% CI: 0.31–0.89), as well as high CD8+ TILS in Human papillomavirus −ve and +ve cancers (HR: 0.39; 95% CI: 0.16–0.93 and HR: 0.40; 95% CI 0.21–0.76 respectively). CD8+ TILs were also associated with improved survival in hypopharyngeal cancers (HR = 0.43 CI: 0.30–0.63). No significant association emerged for patients with cancer of the oral cavity or larynx. Conclusions: The findings from this meta-analysis demonstrate the prognostic significance of CD8+ and CD4+ TILs in HNSCC and variation in tumor subsite warrants further focused investigation. We highlight how TILs may serve as predictive biomarkers to risk stratify patients into treatment groups, with applications in immune-checkpoint inhibitors notable areas for further research.
Molecular Targeted Therapy in Myelodysplastic Syndromes: New Options for Tailored Treatments
Authors: Pagliuca Simona , Gurnari Carmelo , Visconte Valeria
Published: February 2021

Myelodysplastic syndromes (MDS) are a heterogeneous group of clonal hematopoietic disorders characterized by ineffective hematopoiesis, progressive cytopenias and increased risk of transformation to acute myeloid leukemia. The improved understanding of the underlying biology and genetics of MDS has led to better disease and risk classification, paving the way for novel therapeutic opportunities. Indeed, we now have a vast pipeline of targeted agents under pre-clinical and clinical development, potentially able to modify the natural history of the diverse disease spectrum of MDS. Here, we review the latest therapeutic approaches (investigational and approved agents) for MDS treatment. A deep insight will be given to molecularly targeted therapies by reviewing new agents for individualized precision medicine.
Testicular Germ Cell Tumors Acquire Cisplatin Resistance by Rebalancing the Usage of DNA Repair Pathways
Authors: Caggiano Cinzia , Cavallo Francesca , Giannattasio Teresa , Cappelletti Gioia , Rossi Pellegrino , Grimaldi Paola , Feldman Darren R. , Jasin Maria , Barchi Marco
Published: February 2021

Despite germ cell tumors (GCTs) responding to cisplatin-based chemotherapy at a high rate, a subset of patients does not respond to treatment and have significantly worse prognosis. The biological mechanisms underlying the resistance remain unknown. In this study, by using two TGCT cell lines that have acquired cisplatin resistance after chronic exposure to the drug, we identified some key proteins and mechanisms of acquired resistance. We show that cisplatin-resistant cell lines had a non-homologous end-joining (NHEJ)-less phenotype. This correlated with a reduced basal expression of TP53-binding protein 1 (53BP1) and DNA-dependent protein kinase (DNA-PKcs) proteins and reduced formation of 53BP1 foci after cisplatin treatment. Consistent with these observations, modulation of 53BP1 protein expression altered the cell line’s resistance to cisplatin, and inhibition of DNA-PKcs activity antagonized cisplatin cytotoxicity. Dampening of NHEJ was accompanied by a functional increase in the repair of DNA double-strand breaks (DSBs) by the homologous recombination repair pathway. As a result, cisplatin-resistant cells were more resistant to PARP inhibitor (PARPi) monotherapy. Moreover, when PARPi was given in combination with cisplatin, it exerted an additive/synergistic effect, and reduced the cisplatin dose for cytotoxicity. These results suggest that treatment of cisplatin-refractory patients may benefit from low-dose cisplatin therapy combined with PARPi.
Performance of Ultrasound Techniques and the Potential of Artificial Intelligence in the Evaluation of Hepatocellular Carcinoma and Non-Alcoholic Fatty Liver Disease
Authors: Lupsor-Platon Monica , Serban Teodora , Silion Alexandra Iulia , Tirpe George Razvan , Tirpe Alexandru , Florea Mira
Published: February 2021

Global statistics show an increasing percentage of patients that develop non-alcoholic fatty liver disease (NAFLD) and NAFLD-related hepatocellular carcinoma (HCC), even in the absence of cirrhosis. In the present review, we analyzed the diagnostic performance of ultrasonography (US) in the non-invasive evaluation of NAFLD and NAFLD-related HCC, as well as possibilities of optimizing US diagnosis with the help of artificial intelligence (AI) assistance. To date, US is the first-line examination recommended in the screening of patients with clinical suspicion of NAFLD, as it is readily available and leads to a better disease-specific surveillance. However, the conventional US presents limitations that significantly hamper its applicability in quantifying NAFLD and accurately characterizing a given focal liver lesion (FLL). Ultrasound contrast agents (UCAs) are an essential add-on to the conventional B-mode US and to the Doppler US that further empower this method, allowing the evaluation of the enhancement properties and the vascular architecture of FLLs, in comparison to the background parenchyma. The current paper also explores the new universe of AI and the various implications of deep learning algorithms in the evaluation of NAFLD and NAFLD-related HCC through US methods, concluding that it could potentially be a game changer for patient care.
EB1 Restricts Breast Cancer Cell Invadopodia Formation and Matrix Proteolysis via FAK
Authors: Chanez Brice , Ostacolo Kevin , Badache Ali , Thuault Sylvie
Published: February 2021

Regulation of microtubule dynamics by plus-end tracking proteins (+TIPs) plays an essential role in cancer cell migration. However, the role of +TIPs in cancer cell invasion has been poorly addressed. Invadopodia, actin-rich protrusions specialized in extracellular matrix degradation, are essential for cancer cell invasion and metastasis, the leading cause of death in breast cancer. We, therefore, investigated the role of the End Binding protein, EB1, a major hub of the +TIP network, in invadopodia functions. EB1 silencing increased matrix degradation by breast cancer cells. This was recapitulated by depletion of two additional +TIPs and EB1 partners, APC and ACF7, but not by the knockdown of other +TIPs, such as CLASP1/2 or CLIP170. The knockdown of Focal Adhesion Kinase (FAK) was previously proposed to similarly promote invadopodia formation as a consequence of a switch of the Src kinase from focal adhesions to invadopodia. Interestingly, EB1-, APC-, or ACF7-depleted cells had decreased expression/activation of FAK. Remarkably, overexpression of wild type FAK, but not of FAK mutated to prevent Src recruitment, prevented the increased degradative activity induced by EB1 depletion. Overall, we propose that EB1 restricts invadopodia formation through the control of FAK and, consequently, the spatial regulation of Src activity.
Impacts of Environmental Factors on Head and Neck Cancer Pathogenesis and Progression
Authors: Miranda-Galvis Marisol , Loveless Reid , Kowalski Luiz Paulo , Teng Yong
Published: February 2021

Epidemiological and clinical studies over the past two decades have provided strong evidence that genetic elements interacting with environmental components can individually and collectively influence one’s susceptibility to cancer. In addition to tumorigenic properties, numerous environmental factors, such as nutrition, chemical carcinogens, and tobacco/alcohol consumption, possess pro-invasive and pro-metastatic cancer features. In contrast to traditional cancer treatment, modern therapeutics not only take into account an individual’s genetic makeup but also consider gene–environment interactions. The current review sharpens the focus by elaborating on the impact that environmental factors have on the pathogenesis and progression of head and neck cancer and the underlying molecular mechanisms involved. Recent advances, challenges, and future perspectives in this area of research are also discussed. Inhibiting key environmental drivers of tumor progression should yield survival benefits for patients at any stage of head and neck cancer.
Expression of α-Tubulin Acetyltransferase 1 and Tubulin Acetylation as Selective Forces in Cell Competition
Authors: Rahimi Amir Mohammad , Cai Mingfang , Kılıҫ Irem , Kazerouni Zahra Basir , Tapia Contreras Constanza , Hoyer-Fender Sigrid
Published: February 2021

The wound healing response of fibroblasts critically depends on the primary cilium, a sensory organelle protruding into the environment and comprising a stable axonemal structure. A characteristic marker for primary cilia is acetylation of axonemal tubulin. Although formation of primary cilia is under cell cycle control, the environmental cues affecting ciliation are not fully understood. Our purpose was, therefore, to study the impact of culture conditions on cilia formation in NIH3T3 fibroblasts. We quantified ciliation in different NIH3T3 sub-cell lines and culture conditions by immunodetection of primary cilia and counting. Quantitative Western blotting, qRT-PCR, and proliferation assays completed our investigation. We observed large differences between NIH3T3 sub-cell lines in their ability to generate acetylated primary cilia that correlated with cytoplasmic tubulin acetylation. We found no increased activity of the major tubulin deacetylase, HDAC6, but instead reduced expression of the α-tubulin acetyltransferase 1 (Atat1) as being causative. Our observations demonstrate that cells with reduced expression of Atat1 and tubulin acetylation proliferate faster, eventually displacing all other cells in the population. Expression of Atat1 and tubulin acetylation are therefore selective forces in cell competition.
Cellular Mechanisms Participating in Brain Repair of Adult Zebrafish and Mammals after Injury
Authors: Ghaddar Batoul , Lübke Luisa , Couret David , Rastegar Sepand , Diotel Nicolas
Published: February 2021

Adult neurogenesis is an evolutionary conserved process occurring in all vertebrates. However, striking differences are observed between the taxa, considering the number of neurogenic niches, the neural stem cell (NSC) identity, and brain plasticity under constitutive and injury-induced conditions. Zebrafish has become a popular model for the investigation of the molecular and cellular mechanisms involved in adult neurogenesis. Compared to mammals, the adult zebrafish displays a high number of neurogenic niches distributed throughout the brain. Furthermore, it exhibits a strong regenerative capacity without scar formation or any obvious disabilities. In this review, we will first discuss the similarities and differences regarding (i) the distribution of neurogenic niches in the brain of adult zebrafish and mammals (mainly mouse) and (ii) the nature of the neural stem cells within the main telencephalic niches. In the second part, we will describe the cascade of cellular events occurring after telencephalic injury in zebrafish and mouse. Our study clearly shows that most early events happening right after the brain injury are shared between zebrafish and mouse including cell death, microglia, and oligodendrocyte recruitment, as well as injury-induced neurogenesis. In mammals, one of the consequences following an injury is the formation of a glial scar that is persistent. This is not the case in zebrafish, which may be one of the main reasons that zebrafish display a higher regenerative capacity.
Progression of Watermelon Bud Necrosis Virus Infection in Its Vector, Thrips palmi
Authors: Ghosh Amalendu , Priti  , Mandal Bikash , Dietzgen Ralf G.
Published: February 2021

Thrips are important pests of agricultural, horticultural, and forest crops worldwide. In addition to direct damages caused by feeding, several thrips species can transmit diverse tospoviruses. The present understanding of thrips–tospovirus relationships is largely based on studies of tomato spotted wilt virus (TSWV) and Western flower thrips (Frankliniella occidentalis). Little is known about other predominant tospoviruses and their thrips vectors. In this study, we report the progression of watermelon bud necrosis virus (WBNV) infection in its vector, melon thrips (Thrips palmi). Virus infection was visualized in different life stages of thrips using WBNV-nucleocapsid protein antibodies detected with FITC-conjugated secondary antibodies. The anterior midgut was the first to be infected with WBNV in the first instar larvae. The midgut of T. palmi was connected to the principal salivary glands (PSG) via ligaments and the tubular salivary glands (TSG). The infection progressed to the PSG primarily through the connecting ligaments during early larval instars. The TSG may also have an ancillary role in disseminating WBNV from the midgut to PSG in older instars of T. palmi. Infection of WBNV was also spread to the Malpighian tubules, hindgut, and posterior portion of the foregut during the adult stage. Maximum virus-specific fluorescence in the anterior midgut and PSG indicated the primary sites for WBNV replication. These findings will help to better understand the thrips–tospovirus molecular relationships and identify novel potential targets for their management. To our knowledge, this is the first report of the WBNV dissemination path in its vector, T. palmi.
Influence of Nitrogen and Phosphorus on Microalgal Growth, Biomass, Lipid, and Fatty Acid Production: An Overview
Authors: Yaakob Maizatul Azrina , Mohamed Radin Maya Saphira Radin , Al-Gheethi Adel , Aswathnarayana Gokare Ravishankar , Ambati Ranga Rao
Published: February 2021

Microalgae can be used as a source of alternative food, animal feed, biofuel, fertilizer, cosmetics, nutraceuticals and for pharmaceutical purposes. The extraction of organic constituents from microalgae cultivated in the different nutrient compositions is influenced by microalgal growth rates, biomass yield and nutritional content in terms of lipid and fatty acid production. In this context, nutrient composition plays an important role in microalgae cultivation, and depletion and excessive sources of this nutrient might affect the quality of biomass. Investigation on the role of nitrogen and phosphorus, which are crucial for the growth of algae, has been addressed. However, there are challenges for enhancing nutrient utilization efficiently for large scale microalgae cultivation. Hence, this study aims to highlight the level of nitrogen and phosphorus required for microalgae cultivation and focuses on the benefits of nitrogen and phosphorus for increasing biomass productivity of microalgae for improved lipid and fatty acid quantities. Furthermore, the suitable extraction methods that can be used to utilize lipid and fatty acids from microalgae for biofuel have also been reviewed.
Renal Proximal Tubule Cell Cannabinoid-1 Receptor Regulates Bone Remodeling and Mass via a Kidney-to-Bone Axis
Authors: Baraghithy Saja , Soae Yael , Assaf Dekel , Hinden Liad , Udi Shiran , Drori Adi , Gabet Yankel , Tam Joseph
Published: February 2021

The renal proximal tubule cells (RPTCs), well-known for maintaining glucose and mineral homeostasis, play a critical role in the regulation of kidney function and bone remodeling. Deterioration in RPTC function may therefore lead to the development of diabetic kidney disease (DKD) and osteoporosis. Previously, we have shown that the cannabinoid-1 receptor (CB1R) modulates both kidney function as well as bone remodeling and mass via its direct role in RPTCs and bone cells, respectively. Here we employed genetic and pharmacological approaches that target CB1R, and found that its specific nullification in RPTCs preserves bone mass and remodeling both under normo- and hyper-glycemic conditions, and that its chronic blockade prevents the development of diabetes-induced bone loss. These protective effects of negatively targeting CB1R specifically in RPTCs were associated with its ability to modulate erythropoietin (EPO) synthesis, a hormone known to affect bone mass and remodeling. Our findings highlight a novel molecular mechanism by which CB1R in RPTCs remotely regulates skeletal homeostasis via a kidney-to-bone axis that involves EPO.
Adult Upper Cortical Layer Specific Transcription Factor CUX2 Is Expressed in Transient Subplate and Marginal Zone Neurons of the Developing Human Brain
Authors: Miškić Terezija , Kostović Ivica , Rašin Mladen-Roko , Krsnik Željka
Published: February 2021

Cut-Like Homeobox 2 (Cux2) is a transcription factor involved in dendrite and spine development, and synapse formation of projection neurons placed in mouse upper neocortical layers. Therefore, Cux2 is often used as an upper layer marker in the mouse brain. However, expression of its orthologue CUX2 remains unexplored in the human fetal neocortex. Here, we show that CUX2 protein is expressed in transient compartments of developing neocortical anlage during the main fetal phases of neocortical laminar development in human brain. During the early fetal phase when neurons of the upper cortical layers are still radially migrating to reach their final place in the cortical anlage, CUX2 was expressed in the marginal zone (MZ), deep cortical plate, and pre-subplate. During midgestation, CUX2 was still expressed in the migrating upper cortical neurons as well as in the subplate (SP) and MZ neurons. At the term age, CUX2 was expressed in the gyral white matter along with its expected expression in the upper layer neurons. In sum, CUX2 was expressed in migratory neurons of prospective superficial layers and in the diverse subpopulation of transient postmigratory SP and MZ neurons. Therefore, our findings indicate that CUX2 is a novel marker of distinct transient, but critical histogenetic events during corticogenesis. Given the Cux2 functions reported in animal models, our data further suggest that the expression of CUX2 in postmigratory SP and MZ neurons is associated with their unique dendritic and synaptogenesis characteristics.
Chronological Age in Different Bone Development Stages: A Retrospective Comparative Study
Authors: Moca Abel Emanuel , Vaida Luminița Ligia , Moca Rahela Tabita , Țuțuianu Anamaria Violeta , Bochiș Călin Florin , Bochiș Sergiu Alin , Iovanovici Diana Carina , Negruțiu Bianca Maria
Published: February 2021

The assessment of an individual’s development by investigating the skeletal maturity is of much use in various medical fields. Skeletal maturity can be estimated by evaluating the morphology of the cervical vertebrae. The aim of this study was to conduct comparisons of the chronological age in different bone development stages. The retrospective study was conducted based on lateral cephalometric radiographs belonging to patients with ages between 6 and 15.9 years, from Romania. For the assessment of skeletal maturity, the Cervical Vertebral Maturation (CVM) method was used. In total, 356 radiographs were selected, but after applying the exclusion criteria, 252 radiographs remained in the study (178 girls and 74 boys). Different mean chronological age values were obtained for the general sample, as well as for the two genders. The chronological age started to be significantly different at the CS4 stage. Patients with CS4, CS5, and CS6 stages had a significantly higher chronological age compared to patients with CS1, CS2, and CS3 stages. It was noted that patients with CS1 and CS2 stages were more frequently boys, while patients with the CS5 stage were more frequently girls.
Variability of Prognostic Results Based on Biological Parameters in Sickle Cell Disease Cohort Studies in Children: What Should Clinicians Know?
Authors: Sommet Julie , Roux Enora Le , Koehl Bérengère , Haouari Zinedine , Mohamed Damir , Baruchel André , Benkerrou Malika , Alberti Corinne
Published: February 2021

Background: Many pediatric studies describe the association between biological parameters (BP) and severity of sickle cell disease (SCD) using different methods to collect or to analyze BP. This article assesses the methods used for collection and subsequent statistical analysis of BP, and how these impact prognostic results in SCD children cohort studies. Methods: Firstly, we identified the collection and statistical methods used in published SCD cohort studies. Secondly, these methods were applied to our cohort of 375 SCD children, to evaluate the association of BP with cerebral vasculopathy (CV). Results: In 16 cohort studies, BP were collected either once or several times during follow-up. The identified methods in the statistical analysis were: (1) one baseline value per patient (2) last known value; (3) mean of all values; (4) modelling of all values in a two-stage approach. Applying these four different statistical methods to our cohort, the results and interpretation of the association between BP and CV were different depending on the method used. Conclusion: The BP prognostic value depends on the chosen statistical analysis method. Appropriate statistical analyses of prognostic factors in cohort studies should be considered and should enable valuable and reproducible conclusions.
BIM Interoperability Analyses in Structure Design
Authors: Sampaio Alcinia Zita , Gomes Augusto Martins
Published: February 2021

The building information modelling (BIM) methodology supports collaborative works, based on the centralization of all information in a federated BIM model and on an efficient level of interoperability between BIM-based platforms. Concerning the structure design, the interoperability capacity of the most used software presents limitations that must be identified and alternative solutions must be proposed. This study analyzes the process of transfer of structure models between modeling and structure analysis tools. Distinct building cases were performed in order to recognize the type of limitations verified in the transfer processes concerning two-way data flow between several software. The study involves the modeling software ArchiCAD 2020, Revit 2020, and AECOsim 2019 and the structure analyzes tools SAP 2020, Robot 2020, and ETABS 22020. The transfer processes are realized in two ways: using the native data format; using a universal standard data transfer, the Industry Foundation Classes (IFC) format. The level of maturity of BIM in structure design is still relatively low, caused essentially by interoperability problems, but despite the limitations detected, this study shows throughout the development of several building case, that the methodology has clear advantages in the development of the structure project.
Impedance Spectroscopy of Lead-Free Ferroelectric Coatings
Authors: Aleksandrova Mariya , Pandiev Ivailo
Published: February 2021

This paper presents impedance measurements of ferroelectric structures involving lead-free oxide and polymer-oxide composite coatings for sensing and energy harvesting applications. Three different ferroelectric materials grown by conventional microfabrication technologies on solid or flexible substrates are investigated for their basic resonant characteristics. Equivalent electrical circuit models are applied to all cases to explain the electrical behavior of the structures, according to the materials type and thickness. The analytical results show good agreement with the experiments carried out on a basic types of excited thin-film piezoelectric transducers. Additionally, temperature and frequency dependences of the dielectric permittivity and losses are measured for the polymer-oxide composite device in relation with the surface morphology before and after introduction of the polymer to the functional film.
Bodipy-Loaded Micelles Based on Polylactide as Surface Coating for Photodynamic Control of Staphylococcus aureus
Authors: Caruso Enrico , Orlandi Viviana Teresa , Malacarne Miryam Chiara , Martegani Eleonora , Scanferla Chiara , Pappalardo Daniela , Vigliotta Giovanni , Izzo Lorella
Published: February 2021

Decontaminating coating systems (DCSs) represent a challenge against pathogenic bacteria that may colonize hospital surfaces, causing several important infections. In this respect, surface coatings comprising photosensitizers (PSs) are promising but still controversial for several limitations. PSs act through a mechanism of antimicrobial photodynamic inactivation (aPDI) due to formation of reactive oxygen species (ROS) after light irradiation. However, ROS are partially deactivated during their diffusion through a coating matrix; moreover, coatings should allow oxygen penetration that in contact with the activated PS would generate 1O2, an active specie against bacteria. In the attempt to circumvent such constraints, we report a spray DCS made of micelles loaded with a PS belonging to the BODIPY family (2,6-diiodo-1,3,5,7-tetramethyl-8-(2,6-dichlorophenyl)-4,4′-difluoroboradiazaindacene) that is released in a controlled manner and then activated outside the coating. For this aim, we synthesized several amphiphilic copolymers (mPEG–(PLA)n), which form micelles, and established the most stable supramolecular system in terms of critical micelle concentration (CMC) and ∆Gf values. We found that micelles obtained from mPEG–(PLLA)2 were the most thermodynamically stable and able to release BODIPY in a relatively short period of time (about 80% in 6 h). Interestingly, the BODIPY released showed excellent activity against Staphylococcus aureus even at micromolar concentrations.
Toothbrushing Wear Resistance of Stained CAD/CAM Ceramics
Authors: Dal Piva Amanda Maria de Oliveira , Bottino Marco Antonio , Anami Lilian Costa , Werner Arie , Kleverlaan Cornelis Johannes , Lo Giudice Roberto , Famà Fausto , Silva-Concilio Laís Regiane da , Tribst João Paulo Mendes
Published: February 2021

Aim: To investigate the effect of toothbrushing on different stained Computerd Aided Design/Computer Aided Manufacturing CAD/CAM ceramics. Materials and Methods: Fifty specimens (high translucency zirconia, YZHT; zirconia-reinforced lithium silicate, ZLS; feldspathic, FDL; hybrid ceramic, HC; all from Vita Zahnfabrik) were divided into five groups according to their staining technique. ZLS allowed the composition of two groups: ZLS1 with crystallization and staining together; ZLS2, with the stain firing after the crystallization. YZHT received a stain firing after the sintering process, and FDL received stain firing directly. The HC was stained with acrylic staining, and was light-cured on its surface. The specimens were brushed in total for 150,000 cycles at 2.45 N with 180 strokes/min. Surface measurements to obtain Rz were performed after 50,000, 100,000 and 150,000 cycles, with five evaluation lines (5 mm) per specimen orthogonal to the brushing direction, covering brushed and unbrushed areas. The wear was analyzed using two-way ANOVA and Tukey tests (α = 5%). Scanning electron microscopy (SEM) was performed to access the surface profile. The wear was affected by material (p < 0.001) and time (p = 0.139). Superior wear (µm) was observed for HC (6.6 ± 4.4; 6.8 ± 4.0; 9.2 ± 3.5) compared to ZLS2 (1.0 ± 0.3; 1.2 ± 2.2; 1.3 ± 0.3), YZHT (1.0 ± 0.1; 1.2 ± 0.3; 1.2 ± 0.3), ZLS1 (0.9 ± 0.1; 1.1 ± 0.5; 1.2 ± 0.3) and FDL (0.9 ± 0.1; 0.9 ± 0.1; 1.0 ± 0.2) after 5, 10 and 15 years of simulation, respectively. SEM showed different wear patters for HC with the removal of the glaze layer. HC showed a higher staining wear rate compared to the glass-based and polycrystalline ceramics after 15 years. The extrinsic characterization of feldspathic ceramic showed its superior longevity compared to the evaluated high-translucency zirconia, zirconia-reinforced lithium silicate, and hybrid ceramic.
Phytotoxic Evaluation of Phytosynthesized Silver Nanoparticles on Lettuce
Authors: Hasan Murtaza , Mehmood Kinza , Mustafa Ghazala , Zafar Ayesha , Tariq Tuba , Hassan Shahbaz Gul , Loomba Suraj , Zia Muhammad , Mazher Abeer , Mahmood Nasir , Shu Xugang
Published: February 2021

The increasing metal release into the environment warrants investigating their impact on plants, which are cornerstones of ecosystems. Here, Lactuca sativa L. (lettuce) seedlings were exposed hydroponically to different concentrations of silver ions and nanoparticles (Ag NPs) for 25 days to evaluate their impact on plant growth. Seedlings taking Ag+ ions showed an increment of 18% in total phenolic content and 12% in total flavonoid content, whereas under Ag NPs, 7% free radical scavenging activity, 12% total phenolic contents (TPC), and 10% total reducing power are increased. An increase in 31% shoot length, 25% chlorophyll, 11% carbohydrate, and 16% protein content of the lettuce plant is observed in response to Ag NPs, while silver nitrate (AgNO3) has a reduced 40% growth. The lettuce plant was most susceptible to toxic effects of Ag+ ions at a lower concentration, i.e., 0.01 mg/L, while Ag NPs showed less toxicity, only when higher concentrations >100 mg/L were applied. Further, biomolecules other than antioxidant enzymes showed higher phytotoxicity for Ag+ ions, followed by Ag NPs with the concentration of 25, 50, and 100 mg/L compared to the control. Thus, moderate concentrations of Ag NPs have a stimulatory effect on seedling growth, while higher concentrations induced inhibitory effects due to the release of Ag+ ions. These results suggest that optimum metallic contents are desirable for the healthier growth of plants in a controlled way.
Protection of Zr Alloy under High-Temperature Air Oxidation: A Multilayer Coating Approach
Authors: Sidelev Dmitrii V. , Syrtanov Maxim S. , Ruchkin Sergey E. , Pirozhkov Alexey V. , Kashkarov Egor B.
Published: February 2021

Metallic Cr and multilayer CrN/Cr coatings with a thickness of 2.5 µm were deposited onto E110 alloy by magnetron sputtering. Oxidation tests in air were performed at 1100 °C for 10–40 min. The gravimetric measurements showed better protective properties of multilayer CrN/Cr coatings in comparison with metallic Cr coating. Multilayer coating prevented fast Cr–Zr inter-diffusion by the formation of a ZrN layer beneath the coating. The appearance of ZrN is caused by interaction with nitrogen formed from the decomposition of CrN to Cr2N phases. Optical microscopy revealed a residual Cr layer for the multilayer CrN (0.25 µm)/Cr (0.25 µm) coating for all the oxidation periods. Additional in situ X-ray diffraction (XRD) studies of coated alloy during linear heating up to 1400 °C showed that the formation of the Cr2Zr phase in the case of multilayer coatings occurred at a higher (~150 °C) temperature compared to metallic Cr. Multilayer coatings can decrease the nitrogen effect for Zr alloy oxidation. Uniform and thinner oxide layers of Zr alloy were observed when the multilayer coatings were applied. The highest oxidation resistance belonged to the CrN/Cr coating with a multilayer step of 0.25 µm.
Facile Preparation of Superhydrophobic Membrane Inspired by Chinese Traditional Hand-Stretched Noodles
Authors: Cai Xiaomei , Huang Junjie , Lu Xizhao , Yang Le , Lin Tianliang , Lei Tingping
Published: February 2021

A facile approach inspired by Chinese traditional hand-stretched noodle-making process has been demonstrated for the preparation of superhydrophobic membrane for the first time. Unlike standard electrospinning, a metal substrate that is covered with superhydrophobic nanopowders is utilized to collect fibers during electrospinning. Experimental results show that the proposed method can make some nanopowders stick on the fiber surface to endow electrospun membranes with superhydrophoboic property, especially as the substrate is heated. This noodle-making-like electrospinning process is believed to provide a novel and simple way for the fabrication of superhydrophobic membrane, which should further broaden the application of electrospinning technique.
Evaporation of Sessile Droplets of Polyelectrolyte/Surfactant Mixtures on Silicon Wafers
Authors: Akanno Andrew , Perrin Lionel , Guzmán Eduardo , Llamas Sara , Starov Victor M. , Ortega Francisco , Rubio Ramón G. , Velarde Manuel G.
Published: February 2021

The wetting and evaporation behavior of droplets of aqueous solutions of mixtures of poly(diallyldimethylammonium chloride) solution, PDADMAC, with two different anionic surfactants, sodium laureth sulfate, SLES, and sodium N-lauroyl N-methyl taurate, SLMT, were studied in terms of the changes of the contact angle θ and contact length L of sessile droplets of the mixtures on silicon wafers at a temperature of 25 °C and different relative humidities in the range of 30–90%. The advancing contact angle θa was found to depend on the surfactant concentration, independent of the relative humidity, with the mixtures containing SLES presenting improved wetting behaviors. Furthermore, a constant droplet contact angle was not observed during evaporation due to pinning of the droplet at the coffee-ring that was formed. The kinetics for the first evaporation stage of the mixture were independent of the relative humidity, with the evaporation behavior being well described in terms of the universal law for evaporation.
Reduced Thermal Conductivity in Ultrafast Laser Heated Silicon Measured by Time-Resolved X-ray Diffraction
Authors: Jo Wonhyuk , Cho Yong Chan , Kim Seongheun , Landahl Eric Carl , Lee Sooheyong
Published: February 2021

We investigate the effect of free carrier dynamics on heat transport in bulk crystalline Silicon following femtosecond optical excitation of varying fluences. By taking advantage of the dense 500 MHz standard fill pattern in the PLS-II storage ring, we perform high angular-resolution X-ray diffraction measurements on nanosecond-to-microsecond time-scales with femtometer spatial sensitivity. We find noticeably slowed lattice recovery at increasingly high excitation intensities. Modeling the temporal evolution of lattice displacements due to the migration of the near surface generated heat into the bulk requires reduced thermal diffusion coefficients. We attribute this pump-fluence dependent thermal transport behavior to two separate effects: first, the enhanced nonradiative recombination of free carriers, and, second, reduced size of the effective heat source in the material. These results demonstrate the capability of time-resolved X-ray scattering as an effective means to explore the connection between charge carrier dynamics and macroscopic transport properties.
Luminescence Intensity Ratio Thermometry with Er3+: Performance Overview
Authors: Ćirić Aleksandar , Gavrilović Tamara , Dramićanin Miroslav D.
Published: February 2021

The figures of merit of luminescence intensity ratio (LIR) thermometry for Er3+ in 40 different crystals and glasses have been calculated and compared. For calculations, the relevant data has been collected from the literature while the missing data were derived from available absorption and emission spectra. The calculated parameters include Judd–Ofelt parameters, refractive indexes, Slater integrals, spin–orbit coupling parameters, reduced matrix elements (RMEs), energy differences between emitting levels used for LIR, absolute, and relative sensitivities. We found a slight variation of RMEs between hosts because of variations in values of Slater integrals and spin–orbit coupling parameters, and we calculated their average values over 40 hosts. The calculations showed that crystals perform better than glasses in Er3+-based thermometry, and we identified hosts that have large values of both absolute and relative sensitivity.
Longitudinal Symptom Burden Trajectories in a Population-Based Cohort of Women with Metastatic Breast Cancer: A Group-Based Trajectory Modeling Analysis
Authors: Budhwani Suman , Moineddin Rahim , Wodchis Walter P. , Zimmermann Camilla , Howell Doris
Published: February 2021

Understanding the symptom burden trajectory for metastatic breast cancer patients can enable the provision of appropriate supportive care for symptom management. The aim of this study was to describe the longitudinal trajectories of symptom burden for metastatic breast cancer patients at the population-level. A cohort of 995 metastatic breast cancer patients with 16,146 Edmonton Symptom Assessment System (ESAS) assessments was constructed using linked population-level health administrative databases. The patient-reported ESAS total symptom distress score (TSDS) was studied over time using group-based trajectory modeling, and covariate influences on trajectory patterns were examined. Cohort patients experienced symptom burden that could be divided into six distinct trajectories. Patients experiencing a higher baseline TSDS were likely to be classified into trajectory groups with high, uncontrolled TSDS within the study follow-up period (χ2 (1, N = 995) = 136.25, p < 0.001). Compared to patients classified in the group trajectory with the highest relative TSDS (Group 6), patients classified in the lowest relative TSDS trajectory group (Group 1) were more likely to not have comorbidities (97.34% (for Groups 1–3) vs. 91.82% (for Group 6); p < 0.05), more likely to receive chemotherapy (86.52% vs. 80.50%; p < 0.05), and less likely to receive palliative care (52.81% vs. 79.25%; p < 0.0001). Receiving radiotherapy was a significant predictor of how symptom burden was experienced in all identified groups. Overall, metastatic breast cancer patients follow heterogeneous symptom burden trajectories over time, with some experiencing a higher, uncontrolled symptom burden. Understanding trajectories can assist in establishing risk-stratified care pathways for patients.