bluuebunny/crossref_metadata_2025 huggingface dataset:
Peering into the team role kaleidoscope: the interplay of personal characteristics and verbal interactions in collaborative problem solving
Authors: Buseyne Siem , Vrijdags Amelie , Said-Metwaly Sameh , Danquigny Thierry , Heutte Jean , Depaepe Fien , Raes Annelies
Published: September 2024

The objective of this study is to explore the relationship between personality and peer-rated team role behavior on the one hand and team role behavior and verbal behavior on the other hand. To achieve this, different data types were collected in fifteen professional teams of four members (N = 60) from various private and public organizations in Flanders, Belgium. Participants’ personalities were assessed using a workplace-contextualized personality questionnaire based on the Big Five, including domains and facets. Typical team role behavior was assessed by the team members using the Team Role Experience and Orientation peer rating system. Verbal interactions of nine of the teams (n = 36) were recorded in an educational lab setting, where participants performed several collaborative problem-solving tasks as part of a training. To process these audio data, a coding scheme for collaborative problem solving and linguistic inquiry and word count were used. We identified robust links and logical correlation patterns between personality traits and typical team role behaviors, complementing prior research that only focused on self-reported team behavior. For instance, a relatively strong correlation was found between Altruism and the Team builder role. Next, the study reveals that role taking within teams is associated with specific verbal interaction patterns. For example, members identified as Organizers were more engaged in responding to others’ ideas and monitoring execution.
Individual cognitive empowerment and in-role performance: a matched-pair study
Authors: Kimpah Jeniboy , Rothmann Sebastiaan , Ibrahim Hazril Izwar , Jaaffar Amar Hisham , Vinahapsari Cinthia Annisa , Wider Walton , Naces Udang Lester
Published: September 2024

The study examines the model fit of individual cognitive empowerment, which includes psychological and environmental factors, and investigates the direct effect of environmental empowerment specifically work methods, work criteria, and work schedules on in-role performance in Malaysia’s Electrical and Electronic (E&E) manufacturing firms. Data were obtained from 173 engineers and 173 managers, matched in pairs, who have worked together for at least 1 year in 73 E&E manufacturing firms. The study found that the model fit of psychological and environmental factors is stronger for individual cognitive empowerment. Work methods show a positive direct effect on in-role performance. However, work schedules and work criteria do not appear to have a direct effect on in-role performance. This study highlights the importance of considering work methods in the engagement between engineer and manager pairs in the E&E manufacturing sector to enhance in-role performance.
Effects of perceived variety-support on middle school students’ learning engagement in physical education: the mediating role of motivation
Authors: Miao Miao , Chai Hongqin , Xue Rui , Wang Qi
Published: September 2024

Introduction

High engagement in physical education (PE) could effectively develop students’ motor competence and promote physical activity, which was significantly important for students’ physical and mental health. Researches had shown that motivation was an important factor in explaining students’ learning engagement, and variety-support as the fourth independent psychological need was a potential factor influencing students’ learning motivation. However, there was a lack of empirical research evidence on the effect of perceived variety-support on middle school students’ learning engagement in PE and the influencing mechanisms. This study aimed to investigate the direct effect of perceived variety-support on learning engagement in PE and the mediating effect of motivation in PE on the relationship.

Methods

A cross-sectional study was conducted and 587 middle school students from Liaoning province filled the paper-and-pencil questionnaires adopting perceived variety-support in PE scale (PVSPES), utrecht work engagement scale-student (UWES-S), and perceived locus of causality in PE scale, which had been proved to have good reliability and validity (294 boys and 293 girls, Mage=13.47 ± 0.94).

Results

The results showed three variables were significantly positively correlated with each other (r = 0.323-0.562 p < 0.01) and perceived variety-support in PE could not only directly promote middle school students’ learning engagement in PE but also indirectly through the mediating effect of motivation in PE.

Discussion

Therefore, in order to better promote students’ participation in PE class, we should pay more attention to satisfy students’ varied PE learning needs and stimulate students’ autonomous learning motivation.
Effect of community attitudes on suicide mortality in South Korea: a nationwide ecological study
Authors: Hong Minseok , Kim Hyesoo , Park C. Hyung Keun , Lee Hyunju , Rhee Sang Jin , Min Sooyeon , Kim Min Ji , Yang Jeong Hun , Song Yoojin , Son Kyunghoon , Ahn Yong Min
Published: September 2024

Background

Attitudes toward suicide are essential in suicide prevention, as suicide is socio-culturally nuanced. Although the relationship between individual attitudes and suicidal behavior has been extensively studied, the effect of community attitudes—aggregated by region—on suicide mortality remains ambiguous. This study explored the association between community attitudes and real-world suicide mortality.

Methods

Data on attitudes toward suicide from the 2018 Korea National Suicide Survey (N = 1500) and individual mortality data from the MicroData Integrated System were obtained. Confirmatory factor analysis supported a factor structure with three factors: “Permissiveness,” “Unjustified behavior,” and “Readiness to help/Preventability.” Thirty regional units in South Korea aggregated the data for ecological analysis. We used negative binomial models to examine the association at the regional level, and stratified analysis by gender and age group was conducted.

Results

“Permissiveness” was associated with reduced suicide rates in a univariate model (P < 0.001). Adjusting for gender, age, and additional sociodemographics did not alter the association. Additionally, this relationship was observed in males and individuals under 60 years of age after stratification. However, “Unjustified Behavior” and “Readiness to help/Preventability” exhibited no significant association with suicide in any model or stratum.

Conclusion

The observed inverse association between permissive community attitudes and suicide contradicts the findings of previous research that links permissive individual attitudes to increased suicidal behavior. Our findings suggest that attitudes may operate differently at the individual and group levels. Although the cross-sectional design and single-country focus of this study warrant further investigation, our findings indicate that attitudes are significant contextual factors in the process of suicide, which could lead to novel approaches in suicide prevention.
Differential item functioning in the autism behavior checklist in children with autism spectrum disorder based on a machine learning approach
Authors: Peng Kanglong , Chen Meng , Zhou Libing , Weng Xiaofang
Published: September 2024

Aim

Our study utilized the Rasch analysis to examine the psychometric properties of the Autism Behavior Checklist (ABC) in children with autism spectrum disorder (ASD).

Methods

A total of 3,319 children (44.77 ± 23.52 months) were included. The Rasch model (RM) was utilized to test the reliability and validity of the ABC. The GPCMlasso model was used to test the differential item functioning (DIF).

Result

The response pattern of this sample showed acceptable fitness to the RM. The analysis supported the unidimensionality assumption of the ABC. Disordered category functions and DIF were found in all items in the ABC. The participants responded to the ABC items differently depending not only on autistic traits but also on age groups, gender, and symptom classifications.

Conclusion

The Rasch analysis produces reliable evidence to support that the ABC can precisely depict clinical ASD symptoms. Differences in population characteristics may cause unnecessary assessment bias and lead to overestimated or underestimated symptom severity. Hence, special consideration for population characteristics is needed in making an ASD diagnosis.
Factors influencing user’s health information discernment abilities in online health communities: based on SEM and fsQCA
Authors: Wei CaiPing , Cai Yufeng , Liu Jianwei , Guo Yi , Wu Xusheng , He Xiaofeng , Hu DeHua
Published: September 2024

Introduction

Online health communities have become the main source for people to obtain health information. However, the existence of poor-quality health information, misinformation, and rumors in online health communities increases the challenges in governing information quality. It not only affects users’ health decisions but also undermines social stability. It is of great significance to explore the factors that affect users’ ability to discern information in online health communities.

Methods

This study integrated the Stimulus-Organism-Response Theory, Information Ecology Theory and the Mindsponge Theory to constructed a model of factors influencing users’ health information discernment abilities in online health communities. A questionnaire was designed based on the variables in the model, and data was collected. Utilizing Structural Equation Modeling (SEM) in conjunction with fuzzy-set Qualitative Comparative Analysis (fsQCA), the study analyzed the complex causal relationships among stimulus factors, user perception, and the health information discernment abilities.

Results

The results revealed that the dimensions of information, information environment, information technology, and information people all positively influenced health information discernment abilities. Four distinct configurations were identified as triggers for users’ health information discernment abilities. The core conditions included information source, informational support, technological security, technological facilitation, and perceived risk. It was also observed that information quality and emotional support can act as substitutes for one another, as can informational support and emotional support.

Discussion

This study provides a new perspective to study the influencing factors of health information discernment abilities of online health community users. It can provide experiences and references for online health community information services, information resource construction and the development of users’ health information discernment abilities.
Machine learning approaches to identify the link between heavy metal exposure and ischemic stroke using the US NHANES data from 2003 to 2018
Authors: Zibibula Yierpan , Tayier Gulifeire , Maimaiti Aierpati , Liu Tianze , Lu Jinshuai
Published: September 2024

Purpose

There is limited understanding of the link between exposure to heavy metals and ischemic stroke (IS). This research aimed to develop efficient and interpretable machine learning (ML) models to associate the relationship between exposure to heavy metals and IS.

Methods

The data of this research were obtained from the National Health and Nutrition Examination Survey (US NHANES, 2003–2018) database. Seven ML models were used to identify IS caused by exposure to heavy metals. To assess the strength of the models, we employed 10-fold cross-validation, the area under the curve (AUC), F1 scores, Brier scores, Matthews correlation coefficient (MCC), precision-recall (PR) curves, and decision curve analysis (DCA) curves. Following these tests, the best-performing model was selected. Finally, the DALEX package was used for feature explanation and decision-making visualization.

Results

A total of 15,575 participants were involved in this study. The best-performing ML models, which included logistic regression (LR) (AUC: 0.796) and XGBoost (AUC: 0.789), were selected. The DALEX package revealed that age, total mercury in blood, poverty-to-income ratio (PIR), and cadmium were the most significant contributors to IS in the logistic regression and XGBoost models.

Conclusion

The logistic regression and XGBoost models showed high efficiency, accuracy, and robustness in identifying associations between heavy metal exposure and IS in NHANES 2003–2018 participants.
Diapause, pollen ball incidence, and overwintering energetics in the alfalfa leafcutting bee, Megachile rotundata
Authors: Singh Preetpal , Rajamohan Arun , Waybright Sarah A. , Dillon Michael E. , Ferrenberg Scott M. , Rinehart Joseph P. , Bowsher Julia H.
Published: September 2024

Introduction

Warming summer temperatures have the potential to harm managed pollinators, impacting both summer performance and overwintering success. The alfalfa leafcutting bee, Megachile rotundata, is a solitary bee used for commercial pollination of alfalfa. M. rotundata undergoes facultative diapause in the prepupal stage. Prepupae that diapause early in the season are exposed to warm temperatures for a longer period of time than the individuals that start diapause closer to fall, which may reduce lipid reserves required for overwintering survival. Warm temperatures may also contribute to pollen ball incidence, which is when a provision is present but there is no sign of a larva in the brood cell. Our goal was to identify factors that regulate diapause and pollen ball incidence and examine effects of pre-wintering field conditions on post-overwintering energy reserves in M. rotundata.

Methods

Nest boxes were installed near Fargo, ND, Laramie, WY, and Las Cruces, NM, which exposed bees to different photoperiods and thermal regimes. Three nest boxes were placed at each site. We monitored nesting conditions and diapause and pollen ball incidence throughout the season. Lipids, sugars, and glycogen reserves were measured in adults after overwintering.

Results and Discussion

Our models indicate that most of the variation in diapause incidence was explained by nest, with individuals within a nest tending to have the same diapause outcome. This suggests that the environmental conditions experienced by the mother, or genetic predisposition, influences offspring diapause. We also found evidence that high cavity temperatures can cause diapause aversion. In addition, our study is the first to link high nesting cavity temperatures to increased pollen ball incidence. Exposure to stressful temperatures during development and early diapause resulted in an increase in adult lipid reserves after overwintering. Adult sugar and glycogen reserves were not affected by exposure to warm temperatures during development and early diapause. In conclusion, maternal effects and temperature were important factors for diapause and pollen ball incidence in M. rotundata with macronutrient reserves similar for early and late season bees.
Meaningful patient and public engagement in dissemination—embedding co-production in dementia research
Authors: de Wolf-Linder Susanne , Kramer Iris , Hersperger Martina , Schubert Maria , Bächi Sonja , Stolz Monika , Wolverson Emma , Ramsenthaler Christina
Published: September 2024

Background

Patient and Public Involvement and Engagement (PPIE) is still underutilised in both dementia research and corresponding dissemination activities.

Aim

To describe the methods, format, and lessons learned in co-creating and co-producing a dissemination strategy for a research project focused on establishing patient-centred outcome measures into routine palliative community care for persons living with dementia (PLWD) and their informal carers.

Materials and methods

A participatory, hybrid-format workshop was conducted to co-create the dissemination strategy with a PPIE group. A video presentation of findings and a list of prompts shared prior to the workshop were used to elicit views on dissemination strategies and knowledge translation. The workshop was followed up with a survey to consolidate the dissemination strategy. Workshop minutes and survey responses were analysed using qualitative thematic analysis.

Results

22 participants from our diverse PPIE group attended the workshop. Two major themes emerged: (a) Knowledge translation: building bridges between research and practise, and (b) Collaboration and dissemination: everyone's voice is needed. Participants suggested critical changes to dissemination methods and materials. Successful knowledge translation depends on a strong evidence base. For this, materials need to be tailored to specific audiences. Everyone's voice needs to be integrated through co-production in dissemination activities by PPIE members to influence societal change. Tailored dissemination activities within a dissemination strategy were co-created spanning all phases of the research cycle.

Discussion

Informing and educating the public and policymakers about the needs of PLWD relies on disseminating and fostering knowledge translation throughout all phases of the research cycle.
Shapeshifting Liquid Metal Droplets Versus Superbugs
Authors: Hayles Andrew , Truong Vi Khanh , Vasilev Krasimir
Published: September 2024

Scientists have found an exciting new way to fight a big problem called bacterial biofilms. Biofilms are made up of billions of bacteria living inside a sticky substance, a bit like a slime monster. Within biofilms, bacteria are protected from medical treatments and the human immune system. To make matters worse, biofilms are an ideal place for the emergence of new forms of bacteria with improved defenses, which are often called superbugs. Biofilms and superbugs make people very sick, and they are increasingly more difficult to treat. However, scientists may have come up with a new solution—a special liquid metal that can change shape with the help of magnets. It turns into tiny ninja-stars and spears that can pierce through the cell walls of bacteria. When this special liquid metal is exposed to a rotating magnet, the tiny stars whizz around like a whirlwind, destroying bacterial biofilm and killing the superbugs inside.
Effects of inspiratory muscle training on lung function parameter in swimmers: a systematic review and meta-analysis
Authors: Carvajal-Tello Nathali , Ortega José Guillermo , Caballero-Lozada Andrés Fabricio , Devia-Quiñonez María Juliana , González-Calzada Isabella , Rojas-Hernández Daniela , Segura-Ordoñez Alejandro
Published: September 2024

Background

This systematic review and meta-analysis aimed to assess the impact of inspiratory muscle training (IMT) on lung function parameters (MIP, MEP, FEV1, and FVC) between both elite and non-elite swimmers.

Methods

We searched for controlled clinical trials (CCT) and prospective longitudinal studies (PLS) in elite and non-elite swimmers following an inspiratory muscle training (IMT) protocol with a standardized device, published between 2012 and 2023. The databases used in the search were PubMed, Science Direct, Scopus, Springer, Cochrane Central Register of Controlled Trials, and Google Scholar. The primary outcome assessed was the impact of IMT on lung function parameters, including MIP, MEP, FEV1, and FVC.

Results

We selected 13 articles involving 277 subjects aged 11–21 years, with 61.4% being male, and 84.6% being elite swimmers. The most commonly used IMT device was the PowerBreathe®, prescribed for 3–12 weeks, 1–2 sessions per day, 3–6 times per week, with 30 repetitions, starting at 50% of MIP and progressing up to 80%. The meta-analysis showed that IMT was associated with a higher MIP (MD = 29.35 cmH2O, 95% CI: 13.04–45.65 cmH2O, p < 0.01) without affecting FEV1 and FVC.

Conclusion

The swimmers that used IMT improved muscle strength, specifically MIP, without changes in MEP, FEV1, and FVC.
Research on Active Trailer Steering Control Strategy of Tractor Semitrailer under Medium-/High-Speed Conditions
Authors: Tang Yuxi , Cai Yingfeng , Liu Ze , Sun Xiaoqiang , Chen Long , Wang Hai , Dong Zhaozhi
Published: September 2024

The study proposes an active trailer steering control method for tractor semitrailers to promote the path tracking effect of the trailer portion as well as lateral stability during lane changing. Firstly, a simplified model of a tractor semitrailer is constructed, and the MAP map is formed based on the genetic algorithm for the identification of the key parameters, which improves the model’s accuracy. Then the tractor and trailer’s yaw rate and sideslip angle at CG are tracked as the control objective and the trailer angle distribution strategy is given. Then the LQR-based corner controller is designed to control the steering actuators of each axle of the trailer. Finally, the effectiveness of the designed control strategy is verified based on the Trucksim/Simulink joint simulation platform and the semi-physical HiL test platform. The simulation results show that the designed controller can effectively improve the path tracking effect of the tractor and the trailer, and at the same time, the lateral stability parameters of the tractor and the trailer are also significantly improved, which improves the driving stability of the tractor semitrailer.
Estimating Cadmium Concentration in Agricultural Soils with ZY1-02D Hyperspectral Data: A Comparative Analysis of Spectral Transformations and Machine Learning Models
Authors: Lv Junwei , Geng Jing , Xu Xuanhong , Yu Yong , Fang Huajun , Guo Yifan , Cheng Shulan
Published: September 2024

The accumulation of cadmium (Cd) in agricultural soils presents a significant threat to crop safety, emphasizing the critical necessity for effective monitoring and management of soil Cd levels. Despite technological advancements, accurately monitoring soil Cd concentrations using satellite hyperspectral technology remains challenging, particularly in efficiently extracting spectral information. In this study, a total of 304 soil samples were collected from agricultural soils surrounding a tungsten mine located in the Xiancha River basin, Jiangxi Province, Southern China. Leveraging hyperspectral data from the ZY1-02D satellite, this research developed a comprehensive framework that evaluates the predictive accuracy of nine spectral transformations across four modeling approaches to estimate soil Cd concentrations. The spectral transformation methods included four logarithmic and reciprocal transformations, two derivative transformations, and three baseline correction and normalization transformations. The four models utilized for predicting soil Cd were partial least squares regression (PLSR), support vector machine (SVM), bidirectional recurrent neural networks (BRNN), and random forest (RF). The results indicated that these spectral transformations markedly enhanced the absorption and reflection features of the spectral curves, accentuating key peaks and troughs. Compared to the original spectral curves, the correlation analysis between the transformed spectra and soil Cd content showed a notable improvement, particularly with derivative transformations. The combination of the first derivative (FD) transformation with the RF model yielded the highest accuracy (R2 = 0.61, RMSE = 0.37 mg/kg, MAE = 0.21 mg/kg). Furthermore, the RF model in multiple spectral transformations exhibited higher suitability for modeling soil Cd content compared to other models. Overall, this research highlights the substantial applicative potential of the ZY1-02D satellite hyperspectral data for detecting soil heavy metals and provides a framework that integrates optimal spectral transformations and modeling techniques to estimate soil Cd contents.
Shaping Soil Properties and Yield of Cereals Using Cover Crops under Conservation Soil Tillage
Authors: Wilczewski Edward , Jug Irena , Szpunar-Krok Ewa , Staniak Mariola , Jug Danijel
Published: September 2024

The aim of this review was to collect current results on the effect of different plants grown as winter and summer cover crops (CC) on the physical, chemical, and biological properties of soil and on the yield of cereal crops grown in a site with CC, using conservation soil tillage. The analyzed studies indicate that CC usually have a positive impact on the physical and biological properties of the soil. Regardless of the plant species used as CC, we can expect an increase in the number of soil microorganisms and an improvement in the activity of soil enzymes. This effect is particularly beneficial in the case of reduced tillage systems. Mixing CC biomass with the topsoil loosens compacted soils and, in the case of light, sandy soils, increasing the capacity of the sorption complex. The size and composition of CC biomass and weather conditions during the vegetation period and during the covering of the soil with plant biomass are of great importance for improving the chemical properties of the soil. A beneficial effect of CC, especially legumes, on the content of the mineral nitrogen in the topsoil is usually observed. Sometimes, an increase in the content of available forms of potassium (K) and/or phosphorus (P) is also achieved. The effect of CC on the content of soil organic carbon (C), total nitrogen (N), or soil pH is less common. CC used in reduced tillage systems can significantly improve the yield and quality of cereal grain, especially when legumes are used as CC in low-fertility soil conditions and at low fertilization levels. However, non-legumes can also play a very positive role in shaping soil properties and improving cereal yield.
One Copy Number Variation within the Angiopoietin-1 Gene Is Associated with Leizhou Black Goat Meat Quality
Authors: Wu Qun , Han Xiaotao , Zhang Yuelang , Liu Hu , Zhou Hanlin , Wang Ke , Han Jiancheng
Published: September 2024

The ANGPT1 gene plays a crucial role in the regulation of angiogenesis and muscle growth, with previous studies identifying copy number variations (CNVs) within this gene among Leizhou black goats. In this study, we investigated three ANGPT1 CNVs in 417 individuals of LZBG using quantitative PCR (qPCR), examining the impact of different CNV types on the ANGPT1 gene expression and their associations with growth and meat quality traits. Notably, the ANGPT1 CNV-1 (ARS1_chr14:24950001-24953600) overlaps with protein-coding regions and conserved domains; its gain-of-copies genotype (copies ≥ 3) was significantly correlated with ANGPT1 mRNA expression in muscle tissue (p < 0.01). Furthermore, the gain-of-copies genotype of CNV-1 demonstrated significant correlations with various phenotypic traits, including carcass weight, body weight, shear stress, chest circumference, and cross-sectional area of longissimus dorsi muscle. These findings indicate that the CNV-1 gain-of-copies genotype in the ANGPT1 gene may serve as a valuable marker for selecting Leizhou black goats exhibiting enhanced growth and muscular development characteristics, thereby holding potential applications in targeted breeding programs aimed at improving meat quality.
Influence of 17α-Methyltestosterone on Morphological Deformities and Pigmentation Development in Juvenile Japanese Eels, Anguilla japonica
Authors: Hwang Ju-Ae , Park Jun Seong , Jeong Hae Seung , Hwang Seong Don
Published: September 2024

17α-methyltestosterone (MT) is a synthetic steroid used to induce masculinization when administered during the larval stage of fish. However, the side effects of MT on eel are still poorly understood and, in this study, we examined the various effects of MT on juvenile eel A. japonica (100.63 ± 8.56 mm total length (TL)). To further investigate growth and sex differentiation, juvenile eels (n = 1000) were exposed to 25 µg/g MT for 6 months. We analyzed growth-related factors, sex steroid hormones, skin pigmentation, and color-related gene expression. Through this study, we found a 90% sex conversion of juvenile eels to males using MT treatment. In the MT-treated eel group (285.97 ± 26.21 mm TL) where sexual maturity was induced, spermatogonia stages were observed in the gonads. In contrast, the control group (395.97 ± 27.72 mm TL) exhibited an 80% immaturity rate, with only 20% of the subjects that were rapidly developing displaying early oogonia. ELISA analysis results showed that the level of growth hormone, which is known to be secreted from spermatogonia, did not change as a result of MT treatment. We confirmed that MT delayed growth and caused morphological changes, particularly a shortened snout length and pigmentation of the fin. The total length, body weight, and snout length were considerably lower in the experimental group than in the control group. In addition, in histological analysis we also observed that some of the MT-treated group (5 out of 10 fish) showed liver atrophy and inflammation, and physiological analysis showed that the cortisol concentration increased in the MT-treated eels. Interestingly, we found that some pigment color-related genes, such as melanocortin 1 receptor (MC1R), tyrosinase (Tyr), and dopachrome tautomerase (DCT), were significantly overexpressed in the fins of MT-treated eels. These results suggest that the treatment of A. japonica larvae with MT induced masculinization but also causes growth side effects from the use of synthetic hormones.
Interaction of Wheat Bran Particle Size and Stimbiotic Supplementation on Growth Performance and Gut Health Parameters in Broilers
Authors: Veluri Shravani , Bedford Mike R. , Gonzalez-Ortiz Gemma , Olukosi Oluyinka Abiona
Published: September 2024

A 42-day study was conducted with 720-day-old Cobb male broiler chicks allocated to treatments in a 3 × 2 factorial, with the factors as wheat bran (WB) inclusion (no WB, 50 g/kg coarse WB, or 50 g/kg fine WB) and stimbiotic (STB) supplementation in corn-based diets. The inclusion of WB (p < 0.05) or STB supplementation (p < 0.05) increased the FCR and feed intake in the day 0–10 phase. During the day 0–28 phase, coarse-WB inclusion increased (p < 0.05) the FCR, compared to fine WB or diets without WB. In the day 0–42 phase, WB marginally decreased weight gain in diets without STB supplementation, but the STB-supplemented diet, weight gain was greater (p < 0.05) the diet with fine WB compared with diets with coarse WB. Fine-WB inclusion increased the ileal nitrogen and energy digestibility determined at day 18 compared to coarse WB or diets without WB. Supplementation with STB (p < 0.05) or fine WB (p < 0.05) inclusion increased the villi height compared to diets without STB supplementation or coarse WB, or the diet without WB. Coarse or fine WB decreased (p < 0.05) cecal branched-chain fatty acids compared to diets without WB. In conclusion, stimbiotic supplementation to fine WB improved the performance and nutrient digestibility of broilers compared to coarse WB with no effects on the caeca total SCFA concentration.
Comparison of Amino Acid Digestibility between Commercial Crossbred Pigs and Mini-Jeju Island Native Pigs
Authors: Jo Hyunwoong , Htoo John Kyaw , Kim Beob Gyun
Published: September 2024

The objectives of this study were to determine the apparent ileal digestibility and standardized ileal digestibility (SID) of crude protein (CP) and amino acids (AA) in feed ingredients, compare the ileal digestibility of CP and AA between commercial crossbred pigs and mini-Jeju Island native pigs (JINP), and develop models for estimating SID of CP and AA for commercial pigs using mini-JINP data. The study involved five crossbred commercial pigs (31.5 ± 1.6 kg of body weight and 11 weeks of age; Landrace × Yorkshire) and five mini-JINP (31.0 ± 3.2 kg body weight and 20 weeks of age). The pigs were surgically equipped with a T-cannula at the end of ileum. Each pig breed was assigned to 5 dietary treatments in a 5 × 10 incomplete Latin square design with 10 periods. Four experimental diets were formulated to contain each of soybean meal, corn gluten feed, copra meal, and sesame expellers as the sole source of nitrogen. A nitrogen-free diet was also prepared to determine basal endogenous losses of CP and AA. No interaction between breed and feed ingredient was observed for the digestibility of CP and all indispensable AA. The SID of CP and all indispensable AA, except Arg, His, and Lys, did not differ between the two breeds of pigs. Prediction equations were developed for SID of CP and AA of commercial pigs using the SID values of mini-JINP: SID of CP (%) = (1.02 × SID of CP in mini-JINP) − 5.20 with r2 = 0.97 and p < 0.05; SID of Lys (%) = (1.12 × SID of Lys in mini-JINP) − 9.10 with r2 = 0.98 and p < 0.05; and SID of Met (%) = (1.08 × SID of Met in mini-JINP) − 4.27 with r2 = 0.96 and p < 0.05. The digestibility for most AA in feedstuffs for commercial pigs can be estimated using data from mini-JINP.
In Vitro Bond Strength of Dentin Treated with Sodium Hypochlorite: Effects of Antioxidant Solutions
Authors: Grazioli Guillermo , de León Cáceres Elisa , Tessore Romina , Lund Rafael , Monjarás-Ávila Ana , Lukomska-Szymanska Monika , Hardan Louis , Bourgi Rim , Cuevas-Suárez Carlos
Published: September 2024

This systematic review aims to evaluate whether the application of antioxidant solutions can enhance the bond strength of resin-based materials to sodium hypochlorite (NaOCl)-treated dentin. This study follows the PICOT strategy: population (sodium hypochlorite-treated dentin), intervention (application of antioxidants), control (distilled water), outcome (bond strength), and type of studies (in vitro studies). The systematic review and meta-analysis were conducted following PRISMA guidelines. Electronic databases were searched for in vitro studies evaluating the effects of antioxidants on bond strength to sodium hypochlorite-treated dentin. Two independent reviewers screened articles, extracted data, and assessed risk of bias. Meta-analyses were performed using a random-effects model to compare standardized mean differences in bond strength between antioxidant pretreatment and control groups. Inclusion criteria consisted of in vitro studies that examined the bond strength of resin-based materials to NaOCl-treated dentin with antioxidant application, while exclusion criteria included studies with incomplete data, those not using a control group, or those that did not directly measure bond strength. From 3041 initial records, 29 studies were included in the qualitative analysis and 25 in the meta-analysis. Ascorbic acid, sodium ascorbate, grape seed extract, green tea, and rosmarinic acid significantly improved bond strength to sodium hypochlorite-treated dentin (p < 0.05). The effectiveness of grape seed extract varied with adhesive system type. Hesperidin, p-toluene sulfonic acid, and sodium thiosulfate did not significantly improve bond strength. Most studies had a high risk of bias. This suggests that the conclusions drawn from these studies should be interpreted with caution, and further research with more robust methodologies may be needed to confirm the findings. In conclusion, this systematic review implies that certain antioxidants can improve bond strength to sodium hypochlorite-treated dentin, with efficacy depending on the specific agent and adhesive system used. Further standardized studies are needed to optimize protocols and confirm these findings.
Activation of Nuclear Factor Erythroid 2-Related Factor-2 by Oxylipin from Mangifera indica Leaves
Authors: Khalil Atif Ali Khan , Woo Min-Seok , Kang Dong-Min , Ahn Mi-Jeong , Kim Jeong-Ah , Yang Heejung , Kim Jung-Hwan
Published: September 2024

Mangifera indica L., a member of the Anacardiaceae family, is widely cultivated across the globe. The leaves of M. indica are renowned for their medicinal properties, attributed to the abundance of bioactive compounds. This study investigated the effects of mango leaf extract on oxidative stress in HeLa cells. Notably, the n-hexane fraction (MLHx) significantly enhanced antioxidant response element (ARE)-luciferase activity at a concentration of 100 µg/mL, surpassing other fractions. MLHx also promoted the expression of HO-1 mRNA by increasing nuclear NRF2 levels. The molecular mechanism of MLHx involves increased phosphorylation of ERK1/2 and stabilization of NRF2. Bioactivity-guided isolation resulted in the identification of six oxylipins: 13(R)-hydroxy-octadeca-(9Z,11E,15Z)-trienoic acid (C-1), 9(R)-hydroxy-octadeca-(10E,12Z,15Z)-trienoic acid (C-2), 13(R)-hydroxy-(9Z,11E)-octadecadienoic acid (C-3), 9(R)-hydroxy-(10E,12Z)-octadecadienoic acid (C-4), 9-oxo-(10E,12E)-octadecadienoic acid (C-5), and 9-oxo-(10E,12Z)-octadecadienoic acid (C-6). These structures were elucidated using comprehensive spectroscopic techniques, including MS and 1H NMR. Additionally, compounds C-7 (9-oxo-(10E,12Z,15Z)-octadecatrienoic acid) and 8 (13-oxo-(9E,11E)-octadecadienoic acid) were characterized by LC-MS/MS mass fragmentation. This study reports the isolation of compounds 1–6 from M. indica for the first time. When tested for their effect on NRF2 activity in HeLa cells, compounds 3, 5, and 6 showed strong stimulation of ARE-luciferase activity in a dose-dependent manner.
Development of Dust Emission Prediction Model for Open-Pit Mines Based on SHPB Experiment and Image Recognition Method
Authors: Du Shanzhou , Chen Hao , Ding Xiaohua , Liao Zhouquan , Lu Xiang
Published: September 2024

Open-pit coal mining offers high resource recovery, excellent safety conditions, and large-scale production. However, the process generates significant dust, leading to occupational diseases such as pneumoconiosis among miners and adversely affecting nearby vegetation through dust deposition, which hinders photosynthesis and causes ecological damage. This limits the transition of open-pit mining to a green, low-carbon model. Among these processes, blasting generates the most dust and has the widest impact range, but the specific amount of dust generated has not yet been thoroughly studied. This study integrates indoor experiments, theoretical analyses, and field tests, employing the Split Hopkinson Pressure Bar (SHPB) system to conduct impact loading tests on coal–rock samples under pressures ranging from 0.13 MPa to 2.0 MPa. The results indicate that as the impact load increases, the proportion of large-sized blocks decreases while smaller fragments and powdered samples increase, signifying intensified sample fragmentation. Using stress wave attenuation theory, this study translates indoor impact loadings to field blast shock waves, revealing the relationship between blasting dust mass fraction and impact pressure. Field tests at the Haerwusu open-pit coal mine validated the formula. Using image recognition technology to analyze post-blast muck-pile fragmentation, the estimated dust production closely matched the calculated values, with an error margin of less than 10%. This formula provides valuable insights for estimating dust production and improving dust control measures during open-pit mine blasting operations.
Aminocarb Exposure Induces Cytotoxicity and Endoplasmic Reticulum Stress-Mediated Apoptosis in Mouse Sustentacular Sertoli Cells: Implications for Male Infertility and Environmental Health
Authors: Moreira Sílvia , Martins Ana D. , Alves Marco G. , Pastor Luis Miguel , Seco-Rovira Vicente , Oliveira Pedro F. , Pereira Maria de Lourdes
Published: September 2024

Exposure to pesticides, poses a significant threat to male fertility by compromising crucial cells involved in spermatogenesis. Aminocarb, is a widely used carbamate insecticide, although its detrimental effects on the male reproductive system, especially on sustentacular Sertoli cells, pivotal for spermatogenesis, remains poorly understood. In this study, we investigated the effects of escalating concentrations of aminocarb on a mouse Sertoli cell line, TM4. Assessments included cytotoxic analysis, mitochondrial biogenesis and membrane potential, expression of apoptotic proteins, caspase-3 activity, and oxidative stress evaluation. Our findings revealed a dose-dependent reduction in the proliferation and viability of TM4 cells following exposure to increasing concentrations of aminocarb. Notably, exposure to 5 μM of aminocarb induced depolarization of mitochondria membrane potential, and a significant decrease in the ratio of phosphorylated eIF2α to total eIF2α, suggesting heightened endoplasmic reticulum stress via the activation of the eIF2α pathway. Moreover, the same aminocarb concentration was demonstrated to increase both caspase-3 protein levels and activity, indicating an apoptotic induction. Collectively, our results demonstrate that aminocarb serves as an apoptotic inducer for mouse sustentacular Sertoli cells in vitro, suggesting its potential to modulate independent pathways of the apoptotic cascade. These findings underscore the deleterious impact of aminocarb on spermatogenic performance and male fertility, highlighting the urgent need for further investigation into its mechanisms of action and mitigation strategies to safeguard male fertility.
Dynamic Metabolic Responses of Resistant and Susceptible Poplar Clones Induced by Hyphantria cunea Feeding
Authors: Wang Zheshu , Qu Liangjian , Fan Zhibin , Hou Luxuan , Hu Jianjun , Wang Lijuan
Published: September 2024

Poplar trees are significant for both economic and ecological purposes, and the fall webworm (Hyphantria cunea Drury) poses a major threat to their plantation in China. The preliminary resistance assessment in the previous research indicated that there were differences in resistance to the insect among these varieties, with ‘2KEN8’ being more resistant and ‘Nankang’ being more susceptible. The present study analyzed the dynamic changes in the defensive enzymes and metabolic profiles of ‘2KEN8’ and ‘Nankang’ at 24 hours post-infestation (hpi), 48 hpi, and 96 hpi. The results demonstrated that at the same time points, compared to susceptible ‘Nankang’, the leaf consumption by H. cunea in ‘2KEN8’ was smaller, and the larval weight gain was slower, exhibiting clear resistance to the insect. Biochemical analysis revealed that the increased activity of the defensive enzymes in ‘2KEN8’ triggered by the feeding of H. cunea was significantly higher than that of ‘Nankang’. Metabolomics analysis indicated that ‘2KEN8’ initiated an earlier and more intense reprogramming of the metabolic profile post-infestation. In the early stages of infestation, the differential metabolites induced in ‘2KEN8’ primarily included phenolic compounds, flavonoids, and unsaturated fatty acids, which are related to the biosynthesis pathways of phenylpropanoids, flavonoids, unsaturated fatty acids, and jasmonates. The present study is helpful for identifying the metabolic biomarkers for inductive resistance to H. cunea and lays a foundation for the further elucidation of the chemical resistance mechanism of poplar trees against this insect.
Tumorspheres as In Vitro Model for Identifying Predictive Chemoresistance and Tumor Aggressiveness Biomarkers in Breast and Colorectal Cancer
Authors: Martinez-Bernabe Toni , Morla-Barcelo Pere Miquel , Melguizo-Salom Lucas , Munar-Gelabert Margalida , Maroto-Blasco Alba , Torrens-Mas Margalida , Oliver Jordi , Roca Pilar , Nadal-Serrano Mercedes , Pons Daniel Gabriel , Sastre-Serra Jorge
Published: September 2024

Chemoresistance remains a major challenge in the treatment of breast and colorectal cancer. For this reason, finding reliable predictive biomarkers of response to chemotherapy has become a significant research focus in recent years. However, validating in vitro results may be problematic due to the outcome heterogeneity. In this study, we evaluate the use of tumorspheres as an in vitro model for validating biomarkers of chemoresistance in breast and colorectal cancer. Our investigation highlights the crucial role of inflammation-related pathways in modulating the response to chemotherapy. Using in silico approaches, we identified specific markers elevated in responders versus non-responders patients. These markers were consistently higher in three-dimensional (3D) tumorsphere models compared to traditional adherent cell culture models. Furthermore, the number of tumorspheres from breast and colorectal cancer cells increased in response to cisplatin and oxaliplatin treatment, respectively, whereas cell viability decreased in adherent cell culture. This differential response underscores the importance of the 3D tumorsphere model in mimicking the tumor microenvironment more accurately than adherent cell culture. The enhanced chemoresistance observed in the 3D tumorspheres model and their correlation of data with the in silico study suggest that 3D culture models are a better option to approach the in vivo model and also to validate in silico data. Our findings indicate that tumorspheres are an ideal model for validating chemoresistance biomarkers and exploring the interplay between inflammation and chemoresistance in breast and colon cancer.
Insights into Population Status and Habitat Patches of Conservation Concern for the Endangered Indian Pangolin (Manis crassicaudata) in Nowshera District, Northwestern Pakistan
Authors: Khattak Romaan Hayat , Ahmad Shakeel , Mehmood Tahir , Dou Hongliang , Gao Haiyang , Sun Song , Hua Yan
Published: September 2024

The Indian pangolin (Manis crassicaudata) stands out among the four surviving species of Asian pangolins, being the sole species present in Pakistan and listed as endangered owing to trafficking and illicit commerce. In the present study, we explored the population status of the Indian pangolin and the existing suitable habitats in Nowshera district, Pakistan. We employed the line transect method to confirm the species presence and subsequent population estimation. In a survey effort of 156 km2, a total of 56 signs of Indian pangolin were recorded within the research area. Amongst the 56 signs, 46 were burrows (living burrows (53.57%) and feeding burrows (28.57%)). Digging was observed nine (16.07%) times, along with one direct sighting (1.7%). Our results revealed a population estimate of only 29 pangolins in the Nowshera district, with a population density of 0.013 individuals/km2. Later, MaxEnt was applied to the species’ presence points, along with climatic and topographical variables. The MaxEnt model accuracy was good (AUC = 0.811). Of the total area studied, 210 km2 (12.01%) were highly suitable and 238 km2 (13.61%) were moderately suitable habitat for the Indian pangolin. To safeguard the fragile population and habitat of the Indian pangolin, we highly suggest strengthening watch and ward and law enforcement in the study area. By adopting a comprehensive approach that addresses both the direct threats to Indian pangolins and the underlying factors driving their decline, we can effectively protect this endangered species and ensure the preservation of its essential habitats for robust conservation.
Functional Role of Piezo1 in the Human Eosinophil Cell Line AML14.3D10: Implications for the Immune and Sensory Nervous Systems
Authors: Hwang Sung-Min , Song Ji-Min , Choi Jung Ju , Jung YunJae , Park Chul-Kyu , Kim Yong Ho
Published: September 2024

Mechanosensitive ion channels, particularly Piezo channels, are widely expressed in various tissues. However, their role in immune cells remains underexplored. Therefore, this study aimed to investigate the functional role of Piezo1 in the human eosinophil cell line AML14.3D10. We detected Piezo1 mRNA expression, but not Piezo2 expression, in these cells, confirming the presence of the Piezo1 protein. Activation of Piezo1 with Yoda1, its specific agonist, resulted in a significant calcium influx, which was inhibited by the Piezo1-specific inhibitor Dooku1, as well as other nonspecific inhibitors (Ruthenium Red, Gd3+, and GsMTx-4). Further analysis revealed that Piezo1 activation modulated the expression and secretion of both pro-inflammatory and anti-inflammatory cytokines in AML14.3D10 cells. Notably, supernatants from Piezo1-activated AML14.3D10 cells enhanced capsaicin and ATP-induced calcium responses in the dorsal root ganglion neurons of mice. These findings elucidate the physiological role of Piezo1 in AML14.3D10 cells and suggest that factors secreted by these cells can modulate the activity of transient receptor potential 1 (TRPV1) and purinergic receptors, which are associated with pain and itch signaling. The results of this study significantly advance our understanding of the function of Piezo1 channels in the immune and sensory nervous systems.