bluuebunny/crossref_metadata_2025 huggingface dataset:
Neonatal Maternal Separation Modifies Proteostasis Marker Expression in the Adult Hippocampus
Authors: Sierra-Fonseca Jorge A. , Hamdan Jameel N. , Cohen Alexis A. , Cardenas Sonia M. , Saucedo Sigifredo , Lodoza Gabriel A. , Gosselink Kristin L.
Published: July 2021

Exposure to early-life stress (ELS) can persistently modify neuronal circuits and functions, and contribute to the expression of misfolded and aggregated proteins that are hallmarks of several neurodegenerative diseases. The healthy brain is able to clear dysfunctional proteins through the ubiquitin-proteasome system (UPS) and the autophagy-lysosomal pathway (ALP). Accumulating evidence indicates that impairment of these pathways contributes to enhanced protein aggregation and neurodegeneration. While stress is a known precipitant of neurological decline, few specific mechanistic links underlying this relationship have been identified. We hypothesized that neonatal maternal separation (MatSep), a well-established model of ELS, has the ability to alter the levels of UPS and ALP components in the brain, and thus has the potential to disrupt proteostasis. The expression of proteostasis-associated protein markers was evaluated by immunoblotting in the hippocampus and cortex of adult Wistar rats that were previously subjected to MatSep. We observed multiple sex- and MatSep-specific changes in the expression of proteins in the ALP, mitophagy, and UPS pathways, particularly in the hippocampus of adult animals. In contrast, MatSep had limited influence on proteostasis marker expression in the cortex of adult animals. Our results indicate that MatSep can selectively modify the intracellular protein degradation machinery in ways that may impact the development and progression of neurodegenerative disease.
Perineuronal Net Receptor PTPσ Regulates Retention of Memories
Authors: Lesnikova Angelina , Casarotto Plinio , Moliner Rafael , Fred Senem Merve , Biojone Caroline , Castrén Eero
Published: July 2021

Perineuronal nets (PNNs) have an important physiological role in the retention of learning by restricting cognitive flexibility. Their deposition peaks after developmental periods of intensive learning, usually in late childhood, and they help in long-term preservation of newly acquired skills and information. Modulation of PNN function by various techniques enhances plasticity and regulates the retention of memories, which may be beneficial when memory persistence entails negative symptoms such as post-traumatic stress disorder (PTSD). In this study, we investigated the role of PTPσ [receptor-type tyrosine-protein phosphatase S, a phosphatase that is activated by binding of chondroitin sulfate proteoglycans (CSPGs) from PNNs] in retention of memories using Novel Object Recognition and Fear Conditioning models. We observed that mice haploinsufficient forPTPRSgene (PTPσ+/–), although having improved short-term object recognition memory, display impaired long-term memory in both Novel Object Recognition and Fear Conditioning paradigm, as compared to WT littermates. However, PTPσ+/–mice did not show any differences in behavioral tests that do not heavily rely on cognitive flexibility, such as Elevated Plus Maze, Open Field, Marble Burying, and Forced Swimming Test. Since PTPσ has been shown to interact with and dephosphorylate TRKB, we investigated activation of this receptor and its downstream pathways in limbic areas known to be associated with memory. We found that phosphorylation of TRKB and PLCγ are increased in the hippocampus, prefrontal cortex, and amygdaloid complex of PTPσ+/–mice, but other TRKB-mediated signaling pathways are not affected. Our data suggest that PTPσ downregulation promotes TRKB phosphorylation in different brain areas, improves short-term memory performance but disrupts long-term memory retention in the tested animal models. Inhibition of PTPσ or disruption of PNN-PTPσ-TRKB complex might be a potential target for disorders where negative modulation of the acquired memories can be beneficial.
Application of CT-Based Radiomics in Discriminating Pancreatic Cystadenomas From Pancreatic Neuroendocrine Tumors Using Machine Learning Methods
Authors: Han Xuejiao , Yang Jing , Luo Jingwen , Chen Pengan , Zhang Zilong , Alu Aqu , Xiao Yinan , Ma Xuelei
Published: July 2021

Objectives

The purpose of this study aimed at investigating the reliability of radiomics features extracted from contrast-enhanced CT in differentiating pancreatic cystadenomas from pancreatic neuroendocrine tumors (PNETs) using machine-learning methods.

Methods

In this study, a total number of 120 patients, including 66 pancreatic cystadenomas patients and 54 PNETs patients were enrolled. Forty-eight radiomic features were extracted from contrast-enhanced CT images using LIFEx software. Five feature selection methods were adopted to determine the appropriate features for classifiers. Then, nine machine learning classifiers were employed to build predictive models. The performance of the forty-five models was evaluated with area under the curve (AUC), accuracy, sensitivity, specificity, and F1 score in the testing group.

Results

The predictive models exhibited reliable ability of differentiating pancreatic cystadenomas from PNETs when combined with suitable selection methods. A combination of DC as the selection method and RF as the classifier, as well as Xgboost+RF, demonstrated the best discriminative ability, with the highest AUC of 0.997 in the testing group.

Conclusions

Radiomics-based machine learning methods might be a noninvasive tool to assist in differentiating pancreatic cystadenomas and PNETs.
Correlation Between Prognostic Biomarker SLC1A5 and Immune Infiltrates in Various Types of Cancers Including Hepatocellular Carcinoma
Authors: Zhao Junsheng , Yang Zhongli , Tu Mingmin , Meng Wei , Gao Hainv , Li Ming D. , Li Lanjuan
Published: July 2021

Background

Solute carrier family 1 member 5 (SLC1A5) is a major glutamine transporter and plays a key role in tumor growth. The main objectives of this study were to visualize the prognostic landscape of SLC1A5 in multiple cancers and determine the relations between SLC1A5 expression and tumor immunity.

Methods

SLC1A5 expression and its effect on tumor prognosis were analyzed using multiple online tools Oncomine, Gene Expression Profiling Interactive Analysis, PrognoScan, and Kaplan-Meier plotter with their own datasets as well as the data from The Cancer Genome Atlas. The correlations between SLC1A5 and tumor immune infiltrates were determined via TIMER.

Results

SLC1A5 expression was significantly higher in several types of cancers, including hepatocellular carcinoma (HCC), compared with corresponding normal tissues. High SLC1A5 expression correlated with poor overall survival and with disease-free survival related to alcohol consumption. Moreover, SLC1A5 expression correlated positively with the numbers of tumor-infiltrating B cells, CD4+ T and CD8+ T cells, macrophages, neutrophils, and dendritic cells in HCC and in lower-grade glioma (LGG). Also, SLC1A5 expression showed strong correlations with diverse immune marker sets in HCC and LGG, indicating its role in regulating tumor immunity.

Conclusions

SLC1A5 represents a useful prognostic biomarker in multiple cancers, and its expression correlates highly with tumor immune-cell infiltration, especially in HCC and LGG.
IDO Inhibition Facilitates Antitumor Immunity of Vγ9Vδ2 T Cells in Triple-Negative Breast Cancer
Authors: Li Peng , Wu Ruan , Li Ke , Yuan Wenhui , Zeng Chuqian , Zhang Yuting , Wang Xiao , Zhu Xinhai , Zhou Jianjun , Li Ping , Gao Yunfei
Published: July 2021

Triple-negative breast cancer (TNBC) escape from immune-mediated destruction was associated with immunosuppressive responses that dampened the activation of tumor-infiltrating CD8 and γδ T cells. TNBC had a higher level of programmed cell death 1-ligand 1 (PD-L1) and indoleamine 2,3-dioxygenase (IDO), compared with other breast cancer subtypes. But, clinical studies have revealed that the response rate of PD-1/PD-L1 antibody for TNBC treatment was relatively low. However, the antitumor responses of human Vγ9Vδ2 T cells or IDO inhibitor in TNBC treatment are unknown. In this study, we found that IDO1 and PD-L1 were highly expressed in TNBC patients. Analysis of the clinical samples demonstrated that Vγ9Vδ2 T cells became exhausted in triple-negative breast cancer patients. And Vγ9Vδ2 T cells combined with αPD-L1 could not further enhance their antitumor responses in vitro and in vivo. However, Vγ9Vδ2 T cells combined with IDO1 inhibitor 1-Methyl-L-tryptophan (1-MT) or Lindrostat showed substantial inhibitory effects on MDA-MB-231 tumor cells. Finally, we found that IDO1 inhibitor promoted T cell’s cytotoxicity by enhancing perforin production. These results converged to suggest the potential application of Vγ9Vδ2 T cells treated with IDO1 inhibitor for TNBC therapy.
Management of Apatinib-Related Adverse Events in Patients With Advanced Osteosarcoma From Four Prospective Trials: Chinese Sarcoma Study Group Experience
Authors: Xie Lu , Xu Jie , Guo Wei , Wang Zhen , Yao Yang , Li Jianmin , Lin Jianhua , Xiao Jianru , Yu Xiuchun , Zhang Weibin , Cai Zhendong , Hua Yingqi , Chen Jing , Shao Zengwu , Wu Di , Wu Sujia , Tu Zhongqi , Zhang Xiaojing
Published: July 2021

Four prospective trials have reported apatinib-related efficacy in osteosarcoma, with a high response rate of 43.2%. Currently, Adverse Events (AEs) have increasingly gained attention, as treatment with multiple tyrosine kinase inhibitors (TKIs) is potentially lifelong. For this reason, a consensus meeting of the Chinese Sarcoma Study Group (CSSG), which is a multidisciplinary panel composed of pediatric, medical and surgical oncologists specializing in sarcoma, nurse specialists, oncological senior pharmacists and gastroenterologists, was held to develop comprehensive guidelines on AEs emerging due to apatinib treatment to better assist in the prevention, management, and understanding of AE development. We summarized all AEs that arose in ≥10% of the participants as well as rare AEs that required extra caution to prevent that were observed in these four published prospective trials and arranged these AEs into 14 disorder systems according to CTCAE 5.0. In this review, we discuss strategies for the management of AEs in patients with advanced osteosarcoma, with the aim of maximizing treatment benefits and minimizing the need for apatinib treatment discontinuation. We also focus on providing recommendations for the prophylaxis and treatment of advanced osteosarcoma using apatinib to achieve optimal outcomes.
Quantitative Prediction of Microsatellite Instability in Colorectal Cancer With Preoperative PET/CT-Based Radiomics
Authors: Li Jiaru , Yang Ziyi , Xin Bowen , Hao Yichao , Wang Lisheng , Song Shaoli , Xu Junyan , Wang Xiuying
Published: July 2021

Objectives

Microsatellite instability (MSI) status is an important hallmark for prognosis prediction and treatment recommendation of colorectal cancer (CRC). To address issues due to the invasiveness of clinical preoperative evaluation of microsatellite status, we investigated the value of preoperative 18F-FDG PET/CT radiomics with machine learning for predicting the microsatellite status of colorectal cancer patients.

Methods

A total of 173 patients that underwent 18F-FDG PET/CT scans before operations were retrospectively analyzed in this study. The microsatellite status for each patient was identified as microsatellite instability-high (MSI-H) or microsatellite stable (MSS), according to the test for mismatch repair gene proteins with immunohistochemical staining methods. There were 2,492 radiomic features in total extracted from 18F-FDG PET/CT imaging. Then, radiomic features were selected through multivariate random forest selection and univariate relevancy tests after handling the imbalanced dataset through the random under-sampling method. Based on the selected features, we constructed a BalancedBagging model based on Adaboost classifiers to identify the MSI status in patients with CRC. The model performance was evaluated by the area under the curve (AUC), sensitivity, specificity, and accuracy on the validation dataset.

Results

The ensemble model was constructed based on two radiomic features and achieved an 82.8% AUC for predicting the MSI status of colorectal cancer patients. The sensitivity, specificity, and accuracy were 83.3, 76.3, and 76.8%, respectively. The significant correlation of the selected two radiomic features with multiple effective clinical features was identified (p < 0.05).

Conclusion

18F-FDG PET/CT radiomics analysis with the machine learning model provided a quantitative, efficient, and non-invasive mechanism for identifying the microsatellite status of colorectal cancer patients, which optimized the treatment decision support.
The Landscape of Genetic Alterations Stratified Prognosis in Oriental Pancreatic Cancer Patients
Authors: Guo Shiwei , Shi Xiaohan , Gao Suizhi , Hou Qunxing , Jiang Lisha , Li Bo , Shen Jing , Wang Huan , Shen Shuo , Zhang GuoXiao , Pan Yaqi , Liu Wuchao , Xu Xiongfei , Zheng Kailian , Shao Zhuo , Jing Wei , Lin Ling , Li Gang , Jin Gang
Published: July 2021

Background

Pancreatic cancer is a life-threatening malignant disease with significant diversity among geographic regions and races leading to distinct carcinogenesis and prognosis. Previous studies mainly focused on Western patients, while the genomic landscape of Oriental patients, especially Chinese, remained less investigated.

Methods

A total of 408 pancreatic cancer patients were enrolled. A panel containing 436 cancer-related genes was used to detect genetic alterations in tumor samples.

Results

We profiled the genomic alteration landscape of pancreatic duct adenocarcinoma (PDAC), intraductal papillary mucinous neoplasm (IPMN), periampullary carcinoma (PVC), and solid-pseudopapillary tumor (SPT). Comparison with a public database revealed specific gene mutations in Oriental PDAC patients including higher mutation rates of DNA damage repair-related genes. Analysis of mutational signatures showed potential heterogenous carcinogenic factors caused by diabetes mellitus. KRAS mutation, especially KRAS G12D mutation, was associated with poor survival, while patients not harboring the 17 significant copy number variations (CNVs) had a better prognosis. We further identified multiple correlations between clinicopathologic variables and genetic mutations, as well as CNVs. Finally, by network-based stratification, three classes of PDAC patients were robustly clustered. Among these, class 1 (characterized by the Fanconi anemia pathway) achieved the best outcome, while class 2 (involved in the platinum drug resistance pathway) suffered from the worst prognosis.

Conclusions

In this study, we reported for the first time the genetic alteration landscape of Oriental PDAC patients identifying many Oriental-specific alterations. The relationship between genetic alterations and clinicopathological factors as well as prognosis demonstrated important genomic impact on tumor biology. This study will help to optimize clinical treatment of Oriental PDAC patients and improve their survival.
The Effects of Erchen Decoction on Gut Microbiota and Lipid Metabolism Disorders in Zucker Diabetic Fatty Rats
Authors: Zhao Tian , Zhan Libin , Zhou Wen , Chen Wanxin , Luo Jintong , Zhang Lijing , Weng Zebin , Zhao Chunyan , Liu Shenlin
Published: July 2021

Obesity is a chronic metabolic disease caused by genetic and environmental factors that has become a serious global health problem. There is evidence that gut microbiota is closely related to the occurrence and development of obesity. Erchen Decoction (ECD), a traditional Chinese medicine, has been widely used for clinical treatment and basic research of obesity and related metabolic diseases in recent years. It can significantly improve insulin resistance (IR) and lipid metabolism disorders. However, there is no microbiological study on its metabolic regulation. In this study, we investigated the effects of ECD on obesity, especially lipid metabolism and the composition and function of gut microbiota in Zucker diabetic fatty (ZDF) rats, and explored the correlation between the biomarkers of gut microbiota and metabolite and host phenotype. The results showed that ECD could reduce body weight, improve IR and lipid metabolism, and reduce the concentration of free fatty acids (FFA) released from white adipose tissue (WAT) due to excessive lipolysis by interfering with the insulin receptor substrate 1 (IRS1)/protein kinase B (AKT)/protein kinase A (PKA)/hormone-sensitive triglyceride lipase (HSL) signaling pathway in ZDF rats. Additionally, ECD gradually adjusted the overall structure of changed gut microbiota, reversed the relative abundance of six genera, and changed the function of gut microbiota by reducing the content of propionic acid, a metabolite of gut microbiota, in ZDF rats. A potentially close relationship between biomarkers, especially Prevotella, Blautia, and Holdemania, propionic acid and host phenotypes were demonstrated through correlation analysis. The results suggested that the beneficial effects of ECD on obesity, especially lipid metabolism disorders, are related to the regulation of gut microbiota in ZDF rats. This provides a basis for further research on the mechanism and clinical application of ECD to improve obesity via gut microbiota.
H2S Donor and Bone Metabolism
Authors: Hao Yanming , Wang Hongzhen , Fang Lingna , Bian Jinsong , Gao Yan , Li Chong
Published: July 2021

Hydrogen sulfide (H2S) has been recognized as the third gasotransmitter, following nitric oxide and carbon monoxide, and it exerts important biological effects in the body. Growing evidence has shown that H2S is involved in many physiological processes in the body. In recent years, much research has been carried out on the role of H2S in bone metabolism. Bone metabolic diseases have been linked to abnormal endogenous H2S functions and metabolism. It has been found that H2S plays an important role in the regulation of bone diseases such as osteoporosis and osteoarthritis. Regulation of H2S on bone metabolism has many interacting signaling pathways at the molecular level, which play an important role in bone formation and absorption. H2S releasing agents (donors) have achieved significant effects in the treatment of metabolic bone diseases such as osteoporosis and osteoarthritis. In addition, H2S donors and related drugs have been widely used as research tools in basic biomedical research and may be explored as potential therapeutic agents in the future. Donors are used to study the mechanism and function of H2S as they release H2S through different mechanisms. Although H2S releasers have biological activity, their function can be inconsistent. Additionally, donors have different H2S release capabilities, which could lead to different effects. Side effects may form with the formation of H2S; however, it is unclear whether these side effects affect the biological effects of H2S. Therefore, it is necessary to study H2S donors in detail. In this review, we summarize the current information about H2S donors related to bone metabolism diseases and discuss some mechanisms and biological applications.
The Depolarization-Evoked, Ca2+-Dependent Release of Exosomes From Mouse Cortical Nerve Endings: New Insights Into Synaptic Transmission
Authors: Olivero Guendalina , Cisani Francesca , Marimpietri Danilo , Di Paolo Daniela , Gagliani Maria Cristina , Podestà Marina , Cortese Katia , Pittaluga Anna
Published: July 2021

Whether exosomes can be actively released from presynaptic nerve terminals is a matter of debate. To address the point, mouse cortical synaptosomes were incubated under basal and depolarizing (25 mM KCl-enriched medium) conditions, and extracellular vesicles were isolated from the synaptosomal supernatants to be characterized by dynamic light scattering, transmission electron microscopy, Western blot, and flow cytometry analyses. The structural and biochemical analysis unveiled that supernatants contain vesicles that have the size and the shape of exosomes, which were immunopositive for the exosomal markers TSG101, flotillin-1, CD63, and CD9. The marker content increased upon the exposure of nerve terminals to the high-KCl stimulus, consistent with an active release of the exosomes from the depolarized synaptosomes. High KCl-induced depolarization elicits the Ca2+-dependent exocytosis of glutamate. Interestingly, the depolarization-evoked release of exosomes from cortical synaptosomes also occurred in a Ca2+-dependent fashion, since the TSG101, CD63, and CD9 contents in the exosomal fraction isolated from supernatants of depolarized synaptosomes were significantly reduced when omitting external Ca2+ ions. Differently, (±)-baclofen (10 µM), which significantly reduced the glutamate exocytosis, did not affect the amount of exosomal markers, suggesting that the GABAB-mediated mechanism does not control the exosome release. Our findings suggest that the exposure of synaptosomes to a depolarizing stimulus elicits a presynaptic release of exosomes that occurs in a Ca2+-dependent fashion. The insensitivity to the presynaptic GABAB receptors, however, leaves open the question on whether the release of exosomes could be a druggable target for new therapeutic intervention for the cure of synaptopathies.
Colony-Stimulating Factors on Mobilizing CD34+ Cells and Improving Neurological Functions in Patients With Stroke: A Meta-Analysis and a Systematic Review
Authors: Chen Xiuqi , Sun Wenbo , Zhong Ping , Wu Danhong
Published: July 2021

Background and Purpose: CSF therapy is considered a promising therapeutic approach for stroke. We performed a meta-analysis to explore the safety and efficacy of CSF in published clinical stroke studies.

Methods: We searched articles online and manually. Two reviewers selected studies independently, selecting data based on study quality, characteristics of intervention (administration time, observation time, type, dose, and injection approach of CSF), and the baseline characteristics of patients (age, sex, hypertension, diabetes, smoker, and lipids) were extracted. Main prognosis outcomes were measured as all-cause death in severe adverse events (SAE) and recurrent stroke in SAE. Secondary outcomes were measured as CD34+ cell counts in periphery blood at day 5, National Institutes of Health Stroke Scale (NIHSS), and Barthel index (BI), Side effects of CSF were taken as the indicator of safety. STATA13 software was used to perform the meta-analysis.Keywords: Stroke, Colony-stimulating factor, Meta-analysis, therapy, Neurological Diseases

Results: This meta-analysis involved 485 patients from eight studies. Among them, 475 patients from seven studies were gauged SAE (all-cause death), 393 patients from six studies were checked SAE (recurrent stroke); 137 patients from three studies underwent CD34+ measurement, 389 patients from six studies were tested NIHSS and 307 patients from five studies accessed BI. Compared with the control group, both all-causes death (RR= 1.73, 95%CI= (0.61, 4.92), P=0.735, I2=0.0%) and recurrent stroke (RR= 0.43, 95%CI= (0.14, 1.32), P=0.214, I2=33.1%) present no statistical differences, indicating that the application of CSF does not statistically alter the prognosis of patients with stroke. The application of CSF effectively enhanced CD34+ cell counts in periphery blood at day 5 (SMD= 1.23, 95%CI= (0.54, 1.92), P=0.04, I2=69.0%) but did not statistically impact NIHSS (SMD= −0.40, 95%CI= (−0.93, 0.13), P ≤ 0.001, I2=79.7%) or BI (SMD= 0.04, 95%CI= (−0.38, 0.46), P=0.068, I2=54.3%).

Conclusion: Our study consolidates the security of CSF administration for its exerting no effect on detrimental outcomes. It has proven to be effective in elevating CD34+ cell counts in periphery blood at day 5, indicating CSF may participate in stroke recovery, but its efficacy in stroke recovery remains detected.
A Comparison of Nine Machine Learning Mutagenicity Models and Their Application for Predicting Pyrrolizidine Alkaloids
Authors: Helma Christoph , Schöning Verena , Drewe Jürgen , Boss Philipp
Published: July 2021

Random forest, support vector machine, logistic regression, neural networks and k-nearest neighbor (lazar) algorithms, were applied to a new Salmonella mutagenicity dataset with 8,290 unique chemical structures utilizing MolPrint2D and Chemistry Development Kit (CDK) descriptors. Crossvalidation accuracies of all investigated models ranged from 80 to 85% which is comparable with the interlaboratory variability of the Salmonella mutagenicity assay. Pyrrolizidine alkaloid predictions showed a clear distinction between chemical groups, where otonecines had the highest proportion of positive mutagenicity predictions and monoesters the lowest.
Potentiation of Acetylcholine-Induced Relaxation of Aorta in Male UC Davis Type 2 Diabetes Mellitus (UCD-T2DM) Rats: Sex-Specific Responses
Authors: Akther Farjana , Razan Md Rahatullah , Shaligram Sonali , Graham James L. , Stanhope Kimber L. , Allen Kaitlin N. , Vázquez-Medina José Pablo , Havel Peter J. , Rahimian Roshanak
Published: July 2021

Previous reports suggest that diabetes may differentially affect the vascular beds of females and males. The objectives of this study were to examine whether there were (1) sex differences in aortic function and (2) alterations in the relative contribution of endothelium-derived relaxing factors in modulating aortic reactivity in UC Davis Type 2 Diabetes Mellitus (UCD-T2DM) rats. Endothelium-dependent vasorelaxation (EDV) in response to acetylcholine (ACh) was measured in aortic rings before and after exposure to pharmacological inhibitors. Relaxation responses to sodium nitroprusside were assessed in endothelium-denuded rings. Moreover, contractile responses to phenylephrine (PE) were measured before and after incubation of aortic rings with a nitric oxide synthase (NOS) inhibitor in the presence of indomethacin. Metabolic parameters and expression of molecules associated with vascular and insulin signaling as well as reactive oxygen species generation were determined. Diabetes slightly but significantly impaired EDV in response to ACh in aortas from females but potentiated the relaxation response in males. The potentiation of EDV in diabetic male aortas was accompanied by a traces of nitric oxide (NO)- and prostanoid-independent relaxation and elevated aortic expression of small- and intermediate conductance Ca2+-activated K+ channels in this group. The smooth muscle sensitivity to NO was not altered, whereas the responsiveness to PE was significantly enhanced in aortas of diabetic groups in both sexes. Endothelium-derived NO during smooth muscle contraction, as assessed by the potentiation of the response to PE after NOS inhibition, was reduced in aortas of diabetic rats regardless of sex. Accordingly, decreases in pAkt and peNOS were observed in aortas from diabetic rats in both sexes compared with controls. Our data suggest that a decrease in insulin sensitivity via pAkt-peNOS-dependent signaling and an increase in oxidative stress may contribute to the elevated contractile responses observed in diabetic aortas in both sexes. This study demonstrates that aortic function in UCD-T2DM rats is altered in both sexes. Here, we provide the first evidence of sexual dimorphism in aortic relaxation in UCD-T2DM rats.
Serum Adropin as a Potential Biomarker for Predicting the Development of Type 2 Diabetes Mellitus in Individuals With Metabolic Dysfunction-Associated Fatty Liver Disease
Authors: Li Na , Xie Guomin , Zhou Biao , Qu Aijuan , Meng Hua , Liu Jia , Wang Guang
Published: July 2021

Background: Adropin, a peptide translated from the Energy Homeostasis Associated gene (ENHO), was mainly expressed in the liver and was a regulator in metabolic and energy homeostasis. This study aims to investigate the correlation between adropin and histological characteristics of the liver, and the clinical relevance of adropin in patients with metabolic dysfunction-associated fatty liver disease (MAFLD).

Methods: A total of 62 subjects, including 32 healthy controls and 30 MAFLD patients, were enrolled in this case-control study. The MAFLD patients were further divided into two subgroups, including NGT-M group and T2DM-M group. Serum adropin levels, metabolic parameters and intrahepatic lipids, the liver ENHO mRNA expressions and histological characteristics were investigated.

Results: MAFLD patients showed significantly lower circulating adropin compared with healthy controls (2.02 ± 2.92 vs. 5.52 ± 0.65 ng/mL, P < 0.0001). Subgroup analysis exhibited dramatically declined serum adropin levels in T2DM-M patients compared with NGT-M group (0.51 ± 0.73 vs. 4.00 ± 3.52 ng/mL, P < 0.001). H&E and Oil Red O staining show exacerbated steatohepatitis in T2DM-M patients in contrast with NGT-M group. Furthermore, serum adropin concentrations were negatively correlated with intrahepatic triglyceride (TG), total cholesterol (TC), and NAFLD activity score (NAS) (TG: r = −0.495; TC: r = −0.392; NAS: r = −0.451; all P < 0.05).

Conclusions: MAFLD patients showed significantly lower adropin levels than the healthy controls, especially in T2DM patients. Adropin maybe a potential biomarker for predicting the development of MAFLD, especially in T2DM individuals.
The Response of Lentil (Lens culinaris Medik.) to Soil Moisture and Heat Stress Under Different Dates of Sowing and Foliar Application of Micronutrients
Authors: Venugopalan Visha Kumari , Nath Rajib , Sengupta Kajal , Nalia Arpita , Banerjee Saon , Chandran Malamal A. Sarath , Ibrahimova Ulkar , Dessoky Eldessoky S. , Attia Attia O. , Hassan Mohamed M. , Hossain Akbar
Published: July 2021

Soil moisture and air temperature stress are the two major abiotic factors limiting lentil (Lens culinarisMedik.) growth and productivity in the humid tropics. Field experiments were conducted during winter seasons (November to March) of 2018–2019 and 2019–2020 on clay loam soil (AericHaplaquept) of Eastern India to cultivate rainfed lentil, with residual moisture. The objective was to study the effect of different time of sowing and foliar spray of micronutrients in ameliorating the effect of heat and moisture stress lentil crop experience in its reproductive stage. The study was conducted with two different dates of sowing, November and December, as main plot treatment and micronutrients foliar spray of boron, iron, and zinc either alone or in combination as subplot treatment. No foliar spray treatment was considered as a control. The soil moisture content is depleted from 38 to 18% (sowing to harvest) during November sowing; however, in December sowing, the depletion is from 30 to 15%. The foliar spray of micronutrients helped to have a better canopy cover and thus reduced soil evaporation during the later stages of crop growth when the temperature was beyond the threshold temperature of the crop. Crop growth rate (CGR) and biomass were significantly higher (p≤ 0.05) for November sown crop and with foliar spray of boron and iron (FSB + FE) micronutrients. In the later stages of the crop when the soil moisture started depleting with no precipitation, the canopy temperature increased compared with air temperature, leading to positive values of Stress Degree Days (SDD) index. Delay in sowing reduced the duration by 11.4 days (113.5 vs. 102.1 days), resulting in varied accumulated Growing Degree Days (GDD). FSB + FE resulted in the highest yield in both years (1,436 and 1,439 kg ha−1). The results of the study concluded that the optimum time of sowing and foliar spray of micronutrients may be helpful to alleviate the soil moisture and heat stress for the sustainability of lentil production in the subtropical region.
Approximate Measurement Invariance of Willingness to Sacrifice for the Environment Across 30 Countries: The Importance of Prior Distributions and Their Visualization
Authors: Arts Ingrid , Fang Qixiang , van de Schoot Rens , Meitinger Katharina
Published: July 2021

Nationwide opinions and international attitudes toward climate and environmental change are receiving increasing attention in both scientific and political communities. An often used way to measure these attitudes is by large-scale social surveys. However, the assumption for a valid country comparison, measurement invariance, is often not met, especially when a large number of countries are being compared. This makes a ranking of countries by the mean of a latent variable potentially unstable, and may lead to untrustworthy conclusions. Recently, more liberal approaches to assessing measurement invariance have been proposed, such as the alignment method in combination with Bayesian approximate measurement invariance. However, the effect of prior variances on the assessment procedure and substantive conclusions is often not well understood. In this article, we tested for measurement invariance of the latent variable “willingness to sacrifice for the environment” using Maximum Likelihood Multigroup Confirmatory Factor Analysis and Bayesian approximate measurement invariance, both with and without alignment optimization. For the Bayesian models, we used multiple priors to assess the impact on the rank order stability of countries. The results are visualized in such a way that the effect of different prior variances and models on group means and rankings becomes clear. We show that even when models appear to be a good fit to the data, there might still be an unwanted impact on the rank ordering of countries. From the results, we can conclude that people in Switzerland and South Korea are most motivated to sacrifice for the environment, while people in Latvia are less motivated to sacrifice for the environment.
How to Solve the Time Dilemma? The Influence of Team Temporal Leadership on Team Innovation Performance
Authors: Liu Zhengqiao , Liu Xiliang , Zhang Xianchun
Published: July 2021

Time pressure (TP) is the most common kind of pressure faced by R&D teams. How to improve team innovation performance (TIP) when time resources are insufficient has been a concern of practitioners and scholars. The purpose of this paper is to put forward some suggestions to solve that time dilemma. We conducted a survey based on a sample of 163 teams. In the first-stage survey (time 1), we measured the team temporal leadership (TTL) and TP. In the second-stage survey (time 2), we measured team learning behavior (TLB). TIP was measured in the third-wave survey (time 3). The results are as follows: (i) TTL has a significant positive impact on the TLB and TIP; (ii) TLB plays a mediating role in the relationship between TTL and TIP; and (iii) TP can positively moderate the relationship between TTL and TLB, that is, the promoting effect of TTL on TLB is more pronounced under the higher level of TP. These findings reveal the influence mechanism of TTL on TIP from the perspective of TLB and TP.
Experiences With Online Teaching and Psychological Adjustment of High-School Students at the Onset of the COVID-19 Pandemic in Croatia
Authors: Buško Vesna , Bezinović Petar
Published: July 2021

Vastly changed schooling arrangements due to the COVID-19 crisis have generally limited the opportunities and resources for schools to provide necessary psychological and other support to their students. Given this, all parties involved in the schooling system need to understand the kinds of experiences students have via distance learning and how students adapt to novel living and studying conditions. This study examines the relevant sources of stress that students encounter with regard to online classes, and the frustrations they face due to living in social isolation, as well as how these stressors relate to the measures of students’ emotional wellbeing and psychological adjustment. Data were collected in April 2020 via an online questionnaire administered to a sample of 4,492 students (62.6% female) attending all four grades of high school within two Croatian counties. The results point to the differential effects of particular aspects of online teaching practices on the stressful experiences of students. Furthermore, the results of multivariate analysis of variance and canonical discriminant analyses demonstrated notable gender and grade differences in the structure of students’ emotional and behavioral adaptation to the health crisis. Female students and those predominantly studying at the highest grade reported higher frequency and intensity of short-term emotional and psychosomatic difficulties. Male students in the final grade year scored highest on the dimension defined by the number and intensity of online teaching stressors. The results are interesting in light of hypothetical accounts of adjustment mechanisms based on existing stress theories. Moreover, the findings serve as a basis for teachers’ self-reflection and self-evaluation of schools, which can then lead to designing specific programs of support in learning and adaptation to the new living and studying conditions.
Depression Symptoms Mediate Mismatch Between Perceived Severity of the COVID-19 Pandemic and Preventive Motives
Authors: Park Jiwon , Lee Seungmin , Sul Sunhae , Chung Dongil
Published: July 2021

The present study monitored changes in beliefs about the coronavirus disease 2019 (COVID-19) pandemic, depressive symptoms, and preventive motives between the first and second waves in South Korea using an online survey administered to 1,144 individuals nationally representative for age, gender, and areas of residence. While participants correctly updated their beliefs about the worsening pandemic situations, the perceived importance of social distancing did not change, and their motives to follow prevention measures shifted toward compulsory rather than voluntary motives. This inconsistency appeared to be mediated by depressive symptoms, such that negative belief changes followed by increased depressive symptoms were associated with the decreased perceived importance of social distancing and decreased voluntary motives. Our data highlights the importance of psychological responses to the dynamically evolving pandemic situations in promoting preventive behaviors.
The Influence of Interpersonal Sensitivity on Smartphone Addiction: A Moderated Mediation Model
Authors: Lin Li , Wang Xiaochen , Li Qing , Xia Bingnan , Chen Peihong , Wang Wei
Published: July 2021

Smartphone addiction is a behavioral dependence characterized by excessive or compulsive Internet use and a preoccupation with and loss of control over this use that interferes with an individual’s daily functioning and results in negative mental processes and subsequent social consequences. Smartphone addiction can negatively impact physical and mental health as well as academic performance, sleep quality, and even interpersonal interaction and relationships. Based on the compensatory Internet use theory, this study explores the relationship between interpersonal sensitivity and smartphone addiction in college students and constructed a moderated mediation model. A sample of 881 college students was tested using the Interpersonal Sensitivity Scale, Smart Phone Addiction Scale, Fear of Missing Out Scale, and Relational Self-Construal Scale. We used AMOS 26.0 to conduct a confirmatory factor analysis and employed SPSS 24.0 to test our hypotheses. The results indicated that (1) interpersonal sensitivity was positively related to the fear of missing out and smartphone addiction; (2) the fear of missing out mediated the relationship between interpersonal sensitivity and mobile phone addiction; (3) relational self-construal moderated interpersonal sensitivity and the fear of missing out; and (4) relational self-construal moderated the mediating effect of the fear of missing out on the relationship between interpersonal sensitivity and smartphone addiction. We concluded that the fear of missing out and relational self-construal play a moderated mediation effect on the relationship between smartphone addiction and interpersonal sensitivity. Our findings provided some theoretical implications. Specifically, in addition to proposing a new approach for the study of smartphone addiction, we also introduced a theoretical basis for psychotherapy and intervention of smartphone addiction. In addition, this study also provides some insightful ideas for educational practitioners.
Toward Healthier Cookie Habits: Assessing the Role of Packaging Visual Appearance in the Expectations for Dietary Cookies in Digital Environments
Authors: Reinoso-Carvalho Felipe , Campo Raffaele , De Luca Modesto , Velasco Carlos
Published: July 2021

As we tend to consume more and more via e-commerce platforms, the digital version of a dietary product’s package can be one of the most important touchpoints that the consumer has with such product during the purchasing stage of the consumer’s journey. Hence, a dietary food/drink properly presented via its packaging in e-commerce is key, for example, to nudge consumers toward healthier purchase habits. In this study, we assessed the role of different configurations of visual cues commonly present in a product’s packaging (jar vs. bag, transparent vs. opaque, labeled vs. unlabeled) in the expectations associated with dietary cookies when presented in a digital environment. A between-participants study was conducted where eight different packages with different combinations of the three aforementioned features were digitally evaluated by the participants. The results suggest that the presence (vs. absence) of labeling triggered the highest ratings on most assessed dimensions (product quality, healthiness, lightness, sweetness, crumbliness, price, tastiness, greediness for product, product/packaging liking). Moreover, transparent (vs. opaque) packaging tends to yield higher expectations concerning this product’s quality (i.e., product liking, package liking, greediness), though it has an opposite effect on the expected healthiness for such cookies. Some particular interactions between these three visual cues were also observed and are discussed as part of the obtained results. In summary, our results point to how the visual appearance of packaging can be strategically used in order to potentially nudge consumers toward healthier cookie purchase habits.
Tourism Experience and Construction of Personalized Smart Tourism Program Under Tourist Psychology
Authors: Lan Feiya , Huang Qijun , Zeng Lijin , Guan Xiuming , Xing Dan , Cheng Ziyan
Published: July 2021

The present work aims to boost tourism development in China, grasp the psychology of tourists at any time, and provide personalized tourist services. The research object is the tourism industry in Macau. In particular, tourists' experiences are comprehensively analyzed in terms of dining, living, traveling, sightseeing, shopping, and entertaining as per their psychological changes using approaches including big data analysis, literature analysis, and field investigation. In this case, a model of tourism experience formation path is summarized, and a smart travel solution is proposed based on psychological experience. In the end, specific and feasible suggestions are put forward for the Macau tourism industry. Results demonstrate that the psychology-based smart travel solution exerts a significant impact on tourists' tourism experience. Specifically, the weight of secular tourism experience is 0.523, the weight of aesthetic tourism experience is 0.356, and the weight of stimulating tourism experience is 0.121. Tourists prefer travel destinations with excellent urban security and scenic authenticity. They give the two indexes comprehensive scores of 75.14 points and 73.12 points, respectively. The proposed smart travel solution can grasp the psychology of tourists and enhance their tourism experiences. It has strong practical and guiding significances, which can promote constructing smart travel services in Macau and enhancing tourism experiences.
Internal and External Factors’ Influence on Recycling: Insights From a Laboratory Experiment With Observed Behavior
Authors: Linder Noah , Rosenthal Sonny , Sörqvist Patrik , Barthel Stephan
Published: July 2021

Internal psychological factors, such as intentions and personal norms, are central predictors of pro-environmental behavior in many theoretical models, whereas the influence from external factors such as the physical environment is seldom considered. Even rarer is studying how internal factors interact with the physical context in which decisions take place. In the current study, we addressed the relative influence and interaction of psychological and environmental factors on pro-environmental behavior. A laboratory experiment presented participants (N = 399) with a choice to dispatch a used plastic cup in a recycling or general waste bin after participating in a staged “yogurt taste test.” Results showed how the spatial positioning of bins explained more than half of the variance in recycling behavior whilst self-reported recycling intentions were not related to which bin they used. Rinsing cups (to reduce contamination) before recycling, on the other hand, was related to both behavioral intention and external factors. These results show that even seemingly small differences in a choice context can influence how well internal psychological factors predict behavior and how aspects of the physical environment can assist the alignment of behavior and intentions, as well as steering behavior regardless of motivation.
The Cognitive Model of Negative Symptoms in Schizophrenia: A Hierarchical Component Model With PLS-SEM
Authors: Ebrahimi Ali , Poursharifi Hamid , Dolatshahi Behrooz , Rezaee Omid , Hassanabadi Hamid Reza , Naeem Farooq
Published: July 2021

The cognitive model of negative symptoms suggests that some dysfunctional beliefs mediate the relationship between neurocognitive deficits and negative symptoms and disability. This study tested the hypothesis that dysfunctional performance beliefs mediate neurocognitive deficits, negative symptoms, and disability. We used a hierarchal component model with 85 men patients diagnosed with chronic schizophrenia. Results showed a moderate to strong correlation between dysfunctional performance beliefs, neurocognitive deficits, negative symptoms, and disability. These results support the Hierarchal component model (HCM) of the cognitive model of negative symptoms. Our results indicated that the disability in schizophrenia is mediated through dysfunctional performance beliefs, neurocognitive deficits, and negative symptoms pathway. Further, dysfunctional performance beliefs have a crucial role in this pathway. Therefore, targeting this vicious cycle of dysfunctional beliefs can improve disability in patients with schizophrenia.