bluuebunny/crossref_metadata_2025 huggingface dataset:
Brain-inspired Predictive Coding Improves the Performance of Machine Challenging Tasks
Authors: Lee Jangho , Jo Jeonghee , Lee Byounghwa , Lee Jung-Hoon , Yoon Sungroh
Published: November 2022

Backpropagation has been regarded as the most favorable algorithm for training artificial neural networks. However, it has been criticized for its biological implausibility because its learning mechanism contradicts the human brain. Although backpropagation has achieved super-human performance in various machine learning applications, it often shows limited performance in specific tasks. We collectively referred to such tasks as machine-challenging tasks (MCTs) and aimed to investigate methods to enhance machine learning for MCTs. Specifically, we start with a natural question: Can a learning mechanism that mimics the human brain lead to the improvement of MCT performances? We hypothesized that a learning mechanism replicating the human brain is effective for tasks where machine intelligence is difficult. Multiple experiments corresponding to specific types of MCTs where machine intelligence has room to improve performance were performed using predictive coding, a more biologically plausible learning algorithm than backpropagation. This study regarded incremental learning, long-tailed, and few-shot recognition as representative MCTs. With extensive experiments, we examined the effectiveness of predictive coding that robustly outperformed backpropagation-trained networks for the MCTs. We demonstrated that predictive coding-based incremental learning alleviates the effect of catastrophic forgetting. Next, predictive coding-based learning mitigates the classification bias in long-tailed recognition. Finally, we verified that the network trained with predictive coding could correctly predict corresponding targets with few samples. We analyzed the experimental result by drawing analogies between the properties of predictive coding networks and those of the human brain and discussing the potential of predictive coding networks in general machine learning.
A review of risk concepts and models for predicting the risk of primary stroke
Authors: Hunter Elizabeth , Kelleher John D.
Published: November 2022

Predicting an individual's risk of primary stroke is an important tool that can help to lower the burden of stroke for both the individual and society. There are a number of risk models and risk scores in existence but no review or classification designed to help the reader better understand how models differ and the reasoning behind these differences. In this paper we review the existing literature on primary stroke risk prediction models. From our literature review we identify key similarities and differences in the existing models. We find that models can differ in a number of ways, including the event type, the type of analysis, the model type and the time horizon. Based on these similarities and differences we have created a set of questions and a system to help answer those questions that modelers and readers alike can use to help classify and better understand the existing models as well as help to make necessary decisions when creating a new model.
The κ-opioid receptor-induced autophagy is implicated in stress-driven synaptic alterations
Authors: Karoussiotis Christos , Sotiriou Aggeliki , Polissidis Alexia , Symeonof Alexandra , Papavranoussi-Daponte Danae , Nikoletopoulou Vassiliki , Georgoussi Zafiroula
Published: November 2022

Recent evidence has shown that G protein-coupled receptors (GPCRs) are direct sensors of the autophagic machinery and opioid receptors regulate neuronal plasticity and neurotransmission with an as yet unclarified mechanism. Using in vitro and in vivo experimental approaches, this study aims to clarify the potential role of autophagy and κ-opioid receptor (κ-OR) signaling in synaptic alterations. We hereby demonstrate that the selective κ-OR agonist U50,488H, induces autophagy in a time-and dose-dependent manner in Neuro-2A cells stably expressing the human κ-OR by upregulating microtubule-associated protein Light Chain 3-II (LC3-II), Beclin 1 and Autophagy Related Gene 5 (ATG5). Pretreatment of neuronal cells with pertussis toxin blocked the above κ-OR-mediated cellular responses. Our molecular analysis also revealed a κ-OR-driven upregulation of becn1 gene through ERK1,2-dependent activation of the transcription factor CREB in Neuro-2A cells. Moreover, our studies demonstrated that sub-chronic U50,488H administration in mice causes profound increases of specific autophagic markers in the hippocampus with a concomitant decrease of several pre-and post-synaptic proteins, such as spinophilin, postsynaptic density protein 95 (PSD-95) and synaptosomal associated protein 25 (SNAP25). Finally, using acute stress, a stimulus known to increase the levels of the endogenous κ-OR ligand dynorphin, we are demonstrating that administration of the κ-ΟR selective antagonist, nor-binaltorphimine (norBNI), blocks the induction of autophagy and the stress-evoked reduction of synaptic proteins in the hippocampus. These findings provide novel insights about the essential role of autophagic machinery into the mechanisms through which κ-OR signaling regulates brain plasticity.
Effects of swift heavy ion irradiation and annealing on the microstructure and recrystallizationof SiC pre-implanted with Sr ions
Authors: Abdelbagi H. A. A. , Jafer T. A. O. , Skuratov V. A. , Njoroge E. G. , Mlambo M. , Hlatshwayo T. T. , Malherbe J. B.
Published: November 2022

Polycrystalline SiC wafers were implanted with 360 keV strontium (Sr) ions at room temperature (RT)to a fluence of 2 × 1016 cm−2. Some of the implanted samples were irradiated with xenon (Xe) ions of 167 MeV to a fluence of 3.4 × 1014 cm−2 and 8.4 × 1014 cm−2at RT. The as-implanted and implanted then irradiated samples were vacuum annealed (isochronally) at temperatures ranging from 1,100 to 1,400°C in steps of 100°C for 5 h. Annealing induced modification of the microstructure of the implanted and swift heavy ions (SHIs) irradiated SiC was studied by Raman spectroscopy, scanning electron microscopy (SEM) and backscattering spectrometry (RBS). Sr ions bombardment caused formation of an amorphous layer in SiC, while irradiation by Xe ions led to partial recrystallization of the amorphized layer. After annealing at 1,100°C, the samples with low Sr retained ratio showed full recrystallization, while the samples with high Sr retained ratio showed poor recrystallization. This suggests that the presence of Sr within the implanted region inhibited the recrystallization of SiC. Annealing of the as-implanted samples at temperatures from 1,100°C and 1,200°Cresulted in larger average crystal size compared to the SHIsirradiated samples annealed in the same temperature range. The difference in the average crystal sizes between the as-implanted and SHIs irradiated samples was due to the differences in the nucleation rate per amorphous area in the two samples. Ramanspectroscopy results showedthat the intensity of the LO mode of SiC increases with increasing crystal size. However, several factors such as pores and defects in SiC play a role in the decrease of the LO mode intensity of SiC (even if the average crystal size is large).
Potential effect of dietary zinc intake on telomere length: A cross-sectional study of US adults
Authors: Shi Huanchen , Li Xiaoxuan , Yu Haihong , Shi Wanting , Lin Yue , Zhou Yunping
Published: November 2022

Background

Telomere length, which is related to chronic diseases and premature mortality, is influenced by dietary factors. Zinc is known as a dietary antioxidant micronutrient, however, its impact on telomere length remains unclear.

Objective

We aimed to examine the potential effect of dietary zinc intake on telomere length among middle-aged and older individuals in the US.

Materials and methods

Our study included 3,793 US participants aged 45 years and older from the 1999 to 2002 National Health and Nutrition Examination Survey (NHANES). 24-h dietary recall interviews were employed to evaluate zinc consumption. Leukocyte telomere length was assessed by real-time quantitative polymerase chain reaction (qPCR). We adopted generalized linear models to investigate the effect of dietary zinc intake on telomere length, and subgroup analyses were further applied. We further evaluated the dose-response relationship using restricted cubic spline analysis.

Results

Among the 3,793 participants, the average telomere length was 0.926 ± 0.205 (T/S ratio) or 5509.5 ± 494.9 (bp). After adjusting for major confounders, every 5 mg increment in dietary zinc consumption was related to 0.64% (95% CI: 0.17%, 1.10%) longer telomere length. In the subgroup analyses, significant relationships were found in females (Percentage change: 1.11%; 95% CI: 0.48%, 1.75%), obese (Percentage change: 0.88%; 95% CI: 0.26%, 1.50%), and low energy intake individuals (Percentage change: 0.99%; 95% CI: 0.51%, 1.46%). Additionally, we revealed a positive linear relationship between dietary zinc intake and telomere length (P for non-linearity = 0.636).

Conclusion

Our study revealed that elevated dietary zinc intake was significantly related to longer telomere length among adults aged 45 years and older in the US. And the association was more pronounced in females, obese, and low energy intake individuals.
Case report: A rare case of TBL1XR1-RARB positive acute promyelocytic leukemia in child and review of the literature
Authors: Jiang Mingyan , Li Jinrong , Wu Jianrong , Zhu Yiping , Gao Ju
Published: November 2022

Some forms of acute myelogenous leukemia (AML) share typical morphological and immunophenotypic features of acute promyelocytic leukemia (APL) but are negative for promyelocytic leukemia-retinoic acid receptor alpha (PML-RARA) fusion. These forms of AML are known as variant APL. Some variants of APL present with retinoic acid receptor beta (RARB) fused or rearranged with partner genes. RARB-positive APL is very rare, resistant to all-trans retinoic acid (ATRA), and associated with poor prognosis. Here, we reported one case with TBL1XR1-RARB positive APL, featured by early onset and no apparent bleeding tendency or coagulation dysfunction. This patient was resistant to ATRA and arsenic trioxide (ATO), but was good responsive to conventional chemotherapy for AML. The case report was followed by a literature review.
The impact of the microbiome in cancer: Targeting metabolism of cancer cells and host
Authors: Huang Jia-Ting , Mao Yu-Qin
Published: November 2022

Abnormal metabolic alterations of cancer cells and the host play critical roles in the occurrence and development of tumors. Targeting cancer cells and host metabolism can provide novel diagnosis indicators and intervention targets for tumors. In recent years, it has been found that gut microbiota is involved in the metabolism of the host and cancer cells. Increasingly, gut microbiome and their metabolites have been demonstrated great influence on the tumor formation, prognosis and treatment. Specific gut microbial composition and metabolites are associated with the status of tumor in the host. Interventions on the gut microbiota can exert the protective effects on the tumor, through the manipulation of structure and its related metabolites. This may be the new approach to improve the efficacy of tumor prevention and treatment. Here, we discuss the effects and the underlying mechanisms of gut microbiota and microbial-derived metabolites in tumor progression and treatment.
Prediction of outcomes by diffusion kurtosis imaging in patients with large (≥5 cm) hepatocellular carcinoma after liver resection: A retrospective study
Authors: Qin Yun-Long , Wang Shuai , Chen Fei , Liu Hong-Xiu , Yue Kui-Tao , Wang Xi-Zhen , Ning Hou-Fa , Dong Peng , Yu Xiang-Rong , Wang Guang-Zhi
Published: November 2022

Purpose

To evaluate preoperative diffusion kurtosis imaging (DKI) in predicting the outcomes of large hepatocellular carcinoma (HCC) after liver resection (LR).

Materials and methods

From January 2015 to December 2017, patients with a large (≥5cm) HCC who underwent preoperative DKI were retrospectively reviewed. The correlations of the mean kurtosis (MK), mean diffusivity (MD), and apparent diffusion coefficient (ADC) with microvascular invasion (MVI) or histological grade were analyzed. Cox regression analyses were performed to identify the predictors of recurrence-free survival (RFS) and overall survival (OS). A nomogram to predict RFS was established. P<0.05 was considered as statistically significant.

Results

A total of 97 patients (59 males and 38 females, 56.0 ± 10.9 years) were included in this study. The MK, MD, and ADC values were correlated with MVI or histological grade (P<0.01). With a median follow-up time of 41.2 months (range 12-69 months), 67 patients (69.1%) experienced recurrence and 41 patients (42.3%) were still alive. The median RFS and OS periods after LR were 29 and 45 months, respectively. The 1-, 3-, and 5-year RFS and OS rates were 88.7%, 41.2%, and 21.7% and 99.0%, 68.3%, and 25.6%, respectively. MK (P<0.001), PVT (P<0.001), and ADC (P=0.033) were identified as independent predictor factors for RFS. A nomogram including the MK value for RFS showed the best performance, and the C-index was 0.895.

Conclusion

The MK value obtained from DKI is a potential predictive factor for recurrence and poor survival, which could provide valuable information for guiding the efficacy of LR in patients with large HCC.
Construction and validation of a prediction model of extrahepatic metastasis for hepatocellular carcinoma based on common clinically available data
Authors: Zhou Liuxin , Ren Li , Yu Wenhao , Qi Mengjian , Yuan Jiaqi , Wang Wen , Su Xiaoxia , Yin Fengjiao , Deng Manjun , Wang Haijiu , Long Hongmu , Zeng Jiangchao , Yu Jiajian , Fan Haining , Wang Zhixin
Published: November 2022

Objective

This study aimed to investigate the clinical characteristics and risk factors of patients with hepatocellular carcinoma (HCC) with extrahepatic metastases (EHM) and to establish an effective predictive nomogram.

Methods

Clinical and pathological data from 607 patients with hepatocellular carcinoma admitted to the Affiliated Hospital of Qinghai University between 1 January 2015 and 31 May 2018 were documented, as well as demographics, clinical pathological characteristics, and tumor-related parameters to clarify clinical risk factors for HCC EHM. These risks were selected to build an R-based clinical prediction model. The predictive accuracy and discriminating ability of the model were determined by the concordance index (C-index) and the calibration curve. The results were validated with a bootstrap resample and 151 patients from 1 June 2018 to 31 December 2019 at the same facility.

Results

In multivariate analysis, independent factors for EHM were neutrophils, prothrombin time, tumor number, and size, all of which were selected in the model. The C-index in the EHM prediction model was 0.672 and in the validation cohort was 0.694. In the training cohort and the validation cohort, the calibration curve for the probability of EHM showed good agreement between the nomogram prediction and the actual observation.

Conclusion

The extrahepatic metastasis prediction model of hepatocellular carcinoma constructed in this study has some evaluation capability.
Targeting of non-apoptotic cancer cell death mechanisms by quercetin: Implications in cancer therapy
Authors: Yang Hao , Xu Shan , Tang Lidan , Gong Jinhong , Fang Hufeng , Wei Jifu , Su Dan
Published: November 2022

The ultimate goal of cancer treatment is to kill cancer cells, based on the use of various therapeutic agents, such as chemotherapy, radiotherapy, or targeted therapy drugs. Most drugs exert their therapeutic effects on cancer by targeting apoptosis. However, alterations in apoptosis-related molecules and thus assisting cells to evade death, eventually lead to tumor cell resistance to therapeutic drugs. The increased incidence of non-apoptotic cell death modes such as induced autophagy, mitotic catastrophe, senescence, and necrosis is beneficial to overcoming multidrug resistance mediated by apoptosis resistance in tumor cells. Therefore, investigating the function and mechanism of drug-induced non-apoptotic cell death modes has positive implications for the development of new anti-cancer drugs and therapeutic strategies. Phytochemicals show strong potential as an alternative or complementary medicine for alleviating various types of cancer. Quercetin is a flavonoid compound widely found in the daily diet that demonstrates a significant role in inhibiting numerous human cancers. In addition to direct pro-tumor cell apoptosis, both in vivo and in vitro experiments have shown that quercetin exerts anti-tumor properties by triggering diverse non-apoptotic cell death modes. This review summarized the current status of research on the molecular mechanisms and targets through which quercetin-mediated non-apoptotic mode of cancer cell death, including autophagic cell death, senescence, mitotic catastrophe, ferroptosis, necroptosis, etc.
All optical Q-switched laser based spiking neuron
Authors: Mekemeza-Ona Keshia , Routier Baptiste , Charbonnier Benoît
Published: November 2022

This paper studies theoretically the use of a Q-switch laser with side light injection as a spiking all-optical neuron for photonic spiking neural networks (PSNN). Ordinary differential equations for the multi-section laser are presented, including terms for the side light injection for gain quenching and saturable absorption. The behaviour of the laser mimics that of a spiking neuron with ultrafast pico-second scale response and low power control signals.
Construction of a lactate-related prognostic signature for predicting prognosis after surgical repair for acute type a aortic dissection
Authors: Wang Zhigang , Li Kai , Xu Jingfang , Cheng Xiaofeng , Wang Dongjin
Published: November 2022

Background: Serum lactate is commonly measured in the perioperative period in patients who have undergone surgery for an acute type A aortic dissection (ATAAD). However, conflicting data has been reported as to whether lactate elevation is associated with short-term prognosis. The aim of the current study was to determine the association between perioperative arterial lactate levels and postoperative 30-day mortality.

Methods: Patients who underwent repair of a ATAAD at our institution were retrospectively screened and those with comprehensive measurements of serum lactate before surgery and at 0, 1, 3, 6, 12, and 24 h after surgery in the intensive care unit (ICU) were selected for the analysis. Patients’ demographic features and outcomes were reviewed to determine risk factors associated with 30-day mortality using logistic regression modeling. The association between serum lactate levels at different time points and 30-day mortality were analyzed by receiver-operating characteristic curves.

Results: 513 patients were identified and retrospectively analyzed for this study including 66 patients (12.9%) who died within 30 days after surgery. Patients who died within 30 days after surgery had elevated lactate levels measured before surgery and at 0, 1, 3, 6, 12, and 24 h after their ICU stay. Lactate measured at 24 h post ICU admission (odds ratio, 2.131; 95% confidence interval, 1.346–3.374; p = 0.001) was a predictor of 30-day mortality. The area under the curve (AUC) for 30-day mortality with lactate levels at 12 h and 24 h post ICU stay were 0.820 and 0.805, respectively.

Conclusion: Early elevation of lactate level is correlated with increased 30-day mortality in patients who received ATAAD surgical repair.
The metabolomic differential plasma profile between dialysates. Pursuing to understand the mechanisms of citrate dialysate clinical benefits
Authors: Broseta José Jesús , Roca Marta , Rodríguez-Espinosa Diana , López-Romero Luis Carlos , Gómez-Bori Aina , Cuadrado-Payán Elena , Bea-Granell Sergio , Devesa-Such Ramón , Soldevila Amparo , Sánchez-Pérez Pilar , Hernández-Jaras Julio
Published: November 2022

Background: Currently, bicarbonate-based dialysate needs a buffer to prevent precipitation of bicarbonate salts with the bivalent cations, and acetate at 3–4 mmol/L is the most used. However, citrate is being postulated as a preferred option because of its association with better clinical results by poorly understood mechanisms. In that sense, this hypothesis-generating study aims to identify potential metabolites that could biologically explain these improvements found in patients using citrate dialysate.

Methods: A unicentric, cross-over, prospective untargeted metabolomics study was designed to analyze the differences between two dialysates only differing in their buffer, one containing 4 mmol/L of acetate (AD) and the other 1 mmol/L of citrate (CD). Blood samples were collected in four moments (i.e., pre-, mid-, post-, and 30-min-post-dialysis) and analyzed in an untargeted metabolomics approach based on UPLC-Q-ToF mass spectrometry.

Results: The 31 most discriminant metabolomic variables from the plasma samples of the 21 participants screened by their potential clinical implications show that, after dialysis with CD, some uremic toxins appear to be better cleared, the lysine degradation pathway is affected, and branched-chain amino acids post-dialysis levels are 9–10 times higher than with AD; and, on its part, dialysis with AD affects acylcarnitine clearance.

Conclusion: Although most metabolic changes seen in this study could be attributable to the dialysis treatment itself, this study successfully identifies some metabolic variables that differ between CD and AD, which raise new hypotheses that may unveil the mechanisms involved in the clinical improvements observed with citrate in future research.
Circadian clock—A promising scientific target in oral science
Authors: Feng Guangxia , Zhao Jiajia , Peng Jinfeng , Luo Beibei , Zhang Jiaqi , Chen Lili , Xu Zhi
Published: November 2022

The oral and maxillofacial organs play vital roles in chewing, maintaining facial beauty, and speaking. Almost all physiological processes display circadian rhythms that are driven by the circadian clock, allowing organisms to adapt to the changing environment. In recent years, increasing evidence has shown that the circadian clock system participates in oral and maxillofacial physiological and pathological processes, such as jaw and tooth development, salivary gland function, craniofacial malformations, oral carcinoma and other diseases. However, the roles of the circadian clock in oral science have not yet been comprehensively reviewed. Therefore, This paper provides a systematic and integrated perspective on the function of the circadian clock in the fields of oral science, reviews recent advances in terms of the circadian clock in oral and maxillofacial development and disease, dialectically analyzes the importance of the circadian clock system and circadian rhythm to the activities of oral and maxillofacial tissues, and focuses on analyzing the mechanism of the circadian clock in the maintenance of oral health, affecting the common diseases of the oral and maxillofacial region and the process of oral-related systemic diseases, sums up the chronotherapy and preventive measures for oral-related diseases based on changes in tissue activity circadian rhythms, meanwhile, comes up with a new viewpoint to promote oral health and human health.
Mitochondrial antioxidant SkQ1 decreases inflammation following hemorrhagic shock by protecting myocardial mitochondria
Authors: Jia Bo , Ye Jingjing , Gan Lebin , Li Rui , Zhang Mengwei , Sun Diya , Weng Lin , Xiong Yufei , Xu Jun , Zhang Peng , Huang Wei , Zheng Ming , Wang Tianbing
Published: November 2022

Background: Hemorrhagic shock (HS) is a type of hypovolemic shock characterized by hemodynamic instability, tissue hypoperfusion and cellular hypoxia. In pathophysiology, the gradual accumulation of reactive oxygen species (ROS) damages the mitochondria, leading to irreversible cell damage and the release of endogenous damage-associated molecular patterns (DAMPs) including mitochondrial DAMPs (MTDs), eventually triggering the inflammatory response. The novel mitochondria-targeted antioxidant SkQ1 (Visomitin) effectively eliminate excessive intracellular ROS and exhibits anti-inflammatory effects; however, the specific role of SkQ1 in HS has not yet been explicated.

Methods and results: A 40% fixed-blood-loss HS rat model was established in this study. Transmission electron microscopy showed that after HS, the myocardial mitochondrial ultrastructure was damaged and the mtDNA release in circulation was increased and the differentially expressed genes were significantly enriched in mitochondrial and ROS-related pathways. Mitochondria-targeted antioxidant SkQ1 attenuated the increased ROS induced by HS in myocardial tissues and by oxygen-glucose deprivation (OGD) in cardiomyocytes. Ultrastructurally, SkQ1 protected the myocardial mitochondrial structure and reduced the release of the peripheral blood mtDNA after HS. RNA-seq transcriptome analysis showed that 56.5% of the inflammation-related genes, which altered after HS, could be significantly reversed after SkQ1 treatment. Moreover, ELISA indicated that SkQ1 significantly reversed the HS-induced increases in the TNF-α, IL-6, and MCP-1 protein levels in rat peripheral blood.

Conclusion: HS causes damage to the rat myocardial mitochondrial structure, increases mtDNA release and ROS contents, activates the mitochondrial and ROS-related pathways, and induces systemic inflammatory response. The mitochondrial antioxidant SkQ1 can improve rat myocardial mitochondria ultrastructure, reduce mtDNA and ROS contents, and decrease inflammation by protecting myocardial mitochondria, thereby playing a novel protective role in HS.
Force plate vertical jump scans are not a valid proxy for physical fitness in US special warfare trainees
Authors: Scott W. Casan , Hando Ben R. , Butler Cody R. , Mata John D. , Bryant Jacob F. , Angadi Siddhartha S.
Published: November 2022

Background:The United States Air Force Special Warfare Training Wing (SWTW) administers a comprehensive physical fitness test to active duty Airmen entering the Special Warfare training pipeline. The Sparta Science™ system utilizes proprietary software to analyze the force-time curve of a vertical jump and purports to serve as a proxy for traditional military fitness tests. The Sparta Science™ system produces four proprietary metrics, including the Sparta™ Score, which is correlated to high magnitudes of force production purportedlyperformance.This study investigated how Sparta™ Jump Scans correlate to components of a physical fitness test utilized within the SW training pipeline.

Methods:At the entry and exit of an 8-week Special Warfare Training Wing preparatory course (SW PREP), 643 trainees completed both an initial and final Sparta™ Jump Scan and a Candidate Fitness Test (CFT). The Candidate Fitness Test consists of eight components and tests several different domains of fitness including strength, power, muscular endurance, swimming proficiency, and cardiovascular fitness. Paired t-tests were used to determine if Sparta™ Jump Scan metrics and CFT components changed during SW PREP. Sparta™ Score’s correlation was assessed against every other Sparta™ Jump Scan metric and all CFT fitness measures.

Results:This study found that the Sparta™ Jump Scan metrics decline slightly over SW PREP (p< 0.05; negligible-small effect size), while most CFT measures improve (p< 0.05; small-medium effect size). Changes in Sparta™ Jump Scan metrics did not reflect the changes in CFT performance over SW PREP (r2: 0.00–0.03).

Conclusion:The Sparta™ Score was not correlated to the most tactically-relevant fitness measures (rucking and swimming), and only weakly correlated with the only jumping measure on the fitness test, the standing broad jump.
Co-expression of stress-responsive regulatory genes, MuNAC4, MuWRKY3 and MuMYB96 associated with resistant-traits improves drought adaptation in transgenic groundnut (Arachis hypogaea l.) plants
Authors: Venkatesh Boya , Vennapusa Amaranatha R. , Kumar Nulu Jagadeesh , Jayamma N. , Reddy B. Manohara , Johnson A. M. Anthony , Madhusudan K. V. , Pandurangaiah Merum , Kiranmai K. , Sudhakar Chinta
Published: November 2022

Groundnut, cultivated under rain-fed conditions is prone to yield losses due to intermittent drought stress. Drought tolerance is a complex phenomenon and multiple gene expression required to maintain the cellular tolerance. Transcription factors (TFs) regulate many functional genes involved in tolerance mechanisms. In this study, three stress-responsive regulatory TFs cloned from horse gram, (Macrotyloma uniflorum (Lam) Verdc.), MuMYB96, involved in cuticular wax biosynthesis; MuWRKY3, associated with anti-oxidant defense mechanism and MuNAC4, tangled with lateral root development were simultaneously expressed to enhance drought stress resistance in groundnut (Arachis hypogaea L.). The multigene transgenic groundnut lines showed reduced ROS production, membrane damage, and increased superoxide dismutase (SOD) and ascorbate peroxidase (APX) enzyme activity, evidencing improved antioxidative defense mechanisms under drought stress. Multigene transgenic plants showed lower proline content, increased soluble sugars, epicuticular wax content and higher relative water content suggesting higher maintenance of tissue water status compared to wildype and mock plants. The scanning electron microscopy (SEM) analysis showed a substantial increase in deposition of cuticular waxes and variation in stomatal number in multigene transgenic lines compared to wild type and mock plants. The multigene transgenic plants showed increased growth of lateral roots, chlorophyll content, and stay-green nature in drought stress compared to wild type and mock plants. Expression analysis of transgenes, MuMYB96, MuWRKY3, and MuNAC4 and their downstream target genes, KCS6, KCR1, APX3, CSD1, LBD16 and DBP using qRT-PCR showed a two- to four-fold increase in transcript levels in multigene transgenic groundnut plants over wild type and mock plants under drought stress. Our study demonstrate that introducing multiple genes with simultaneous expression of genes is a viable option to improve stress tolerance and productivity under drought stress.
Emotion recognition dysfunction after anesthesia and cardiac surgery
Authors: Zhang Delin , Shen Yi , Chen Zhiyun , Guo Yang , Gao Zaifeng , Huang Jian , Lu Xiqian
Published: November 2022

Cognitive dysfunction after anesthesia and surgery has long been recognized. Recently, researchers provided empirical evidence for social cognition dysfunction (SCD) after anesthesia and surgery. In the present study, we concentrated on the deficits in emotion recognition, one of the most important clinical perspectives in SCD, in patients who underwent cardiac surgery. Biological motion (BM) was considered as the stimulus of interest, and patients’ abilities of BM emotion perception and action perception before and after anesthesia and surgery were examined. In total, 60 adult patients (40–72 years old) completed the BM recognition task, which required them to label the types of actions and emotions of perceived BM. The results showed that while action perception remained intact after cardiac surgery, 18.3% of patients exhibited deficits in emotion perception, further confirming the existence of SCD after anesthesia and surgery.
Influence of adolescents’ tendency to catastrophise on non-suicidal self-injury behaviour: A moderated mediation model
Authors: Lan Zhensong , Pau Kee , Yusof Hapsah Md , Zhao Qi , Liang Fangmei , Huang Xuefang
Published: November 2022

Adolescence is the peak period of non-suicidal self-injury (NSSI) behaviour, and it is also a period when the prevention and intervention in NSSI are frequently required. To explore the relationship between catastrophising and NSSI and its mechanism, the mediating effects of parental support and negative emotions on catastrophising and NSSI were analysed. A questionnaire was administered to 401 middle school students with a history of NSSI behaviour from 12 schools in Guangxi. (1) Adolescents’ catastrophising tendencies positively predicted their NSSI status through negative emotions (B = 0.274, P < 0.001). The more severe the adolescents’ catastrophising tendency, the more likely they were to have stronger negative emotions and thus show an increased NSSI level. (2) Parental support played a positive moderating role between the tendency to catastrophise and negative emotions (B = −0.101, t = −2.694, P < 0.01), and it had a protective effect on adolescents’ NSSI. Catastrophising was less likely to induce the NSSI behaviour by reducing adolescents’ negative emotions (B = −0.104, t = −2.313, P < 0.05); however, it should be noted that only parental affective support, informative support, and instrumental support played a significant mediating role. Parental support of adolescents has positive effects on the tendency to catastrophise, negative emotions, and NSSI, and it has some implications for the prevention and intervention of adolescents’ NSSI.
Perceived stress and mobile phone addiction among college students: The roles of self-control and security
Authors: Zhang Anqi , Xiong Sicheng , Peng Yu , Zeng Yixin , Zeng Chengwei , Yang Ying , Zhang Bin
Published: November 2022

Objective

According to the General Strain Theory, stress can lead to a range of problem behaviors. In the current study, we focused on the association between perceived stress and mobile phone addiction. We hypothesized that this association is mediated by low self-control and that the first path of the mediation is moderated by security.

Methods

College students (N = 397; ages 16–21; 51.89% females) from a university in Hunan Province, China, were surveyed by cluster sampling method. The students completed the Smartphone Addiction Scale-Short Version (SAS-SV), the Depression Anxiety Stress Scale (DASS-21), the Self-Control Scale (SCS), and the Security Questionnaire (SQ) during regular class time. SPSS26.0 statistical software was used for descriptive statistics and Pearson correlation analyses, the SPSS macro PROCESS was used to test the mediating effects of self-control and the moderating role of security.

Results

Mediation analysis showed that as expected, perceived stress was associated with lower self-control, which in turn was associated with a higher risk for mobile phone addiction. Also as expected, moderated mediation analysis indicated that the association between perceived stress and self-control was moderated by security. Specifically, the relationship between perceived stress and self-control was stronger for low security.

Conclusion

This study provides useful insight into the understanding of how perceived stress increases the risk of mobile phone addiction. The results are consistent with the General Strain Theory and further indicate that concrete approaches are required for the prevention and intervention to reduce mobile phone addiction among college students.
The association between violent video game exposure and sub-types of school bullying in Chinese adolescents
Authors: She Yuhang , Yang Zidan , Xu Lingyu , Li Liping
Published: November 2022

Background

School bullying among adolescents has been a worldwide public health issue. It has been observed that adolescents who are exposed to violent video games (VVGs) are often more aggressive. However, research on the association between violent video game exposure (VVGE) and different types of school bullying is limited in the Chinese context.

Objective

The purpose of this study was to explore whether VVGE is linked to school bullying behaviors among Chinese adolescents and to examine the relationship between different levels of violent game exposure and four sub-types (physical, verbal, relational, and cyber) of school bullying involvement.

Methods

This was a cross-sectional study of 1,992 Chinese students (55.02% boys and 44.98% girls) with the average age of 15.84 ± 1.62 years. Sub-types of school bullying victimization and perpetration, Internet addiction, and VVGE were measured by using a self-administrated questionnaire. The association was examined by multiple logistic regression analysis, adjusting for covariates.

Results

Physical, verbal, relational, and cyber school bullying victimization were reported by 18.12, 60.34, 11.75, and 12.05% of the adolescents, and physical, verbal, relational, and cyber school bullying perpetration were reported by 16.62, 54.62, 21.49, and 8.23% of them. Of the students, 1,398 (70.18%) were normal Internet users, 514 (25.80%) showed moderate Internet addictive behaviors, and 31 (1.56%) of the students showed severe Internet addictive behaviors. The prevalence of no VVGE, low-level VVGE, medium-level VVGE, and high-level of VVGE were 27.70, 24.10, 24.20, and 24.00%, respectively. The risk of physical victimization and physical perpetration significantly increased with the increasing degree of violent video game exposure (P for trend < 0.001), with the highest adjusted odds ratios (ORs) of 2.251 (95% CI 1.501–3.375) and 2.554 (95% CI 1.685–3.870), when comparing high-level VVGE with no VVGE.

Conclusion

These findings highlight the specific association between different sub-types of school bullying involvement and violent video game exposure. Physical school bullying prevention and intervention programs should be conducted after adolescents are exposed to violent video games.
Cannabis use and opioid relapse: An exploratory survival analysis of prospectively collected data
Authors: Naji Leen , Rosic Tea , Sanger Nitika , Dennis Brittany , Hillmer Alannah , Hudson Jacqueline , Worster Andrew , Paul James , Marsh David C. , Thabane Lehana , Samaan Zainab
Published: November 2022

Importance

It is known that only minority of patients with opioid use disorder (OUD) receive treatment, of which only a fraction successfully complete treatment as intended. Factors associated with poor treatment outcomes remain unclear, and there is emerging but conflicting evidence that cannabis use may mitigate opioid use.

Objective

To analyze predictors of relapse amongst patients receiving buprenorphine-naloxone for OUD and identify the association between cannabis use and time to relapse.

Design

Data were prospectively collected between May 2018 and October 2020, and patients were followed for 12 months.

Setting

Thirty-one outpatient opioid agonist treatment clinics across Ontario, Canada.

Participants

All patients 16 years of age or older receiving buprenorphine-naloxone for OUD who had a urine toxicology screen negative for opioids at baseline were eligible for inclusion. Of the 488 patients consecutively sampled, 466 were included.

Exposure

Cannabis use.

Main outcome and measure

Relapse to opioid use assessed using urine toxicology screens. We employed a multivariable Cox-proportional hazard model for our analyses.

Results

We found that cannabis use was not protective against relapse [hazard ratio (HR) = 1.03, 95% confidence interval (CI): 0.78, 1.36, p = 0.84]. We found that participants who have been in treatment for at least two years had a 44% decrease in the hazard of relapse compared to those in treatment for less than a year (HR = 0.56, 95% CI: 0.34, 0.92, p = 0.021). We also found that the hazard of relapse was 2.6 times higher for participants who were intravenous drug users (HR = 2.61, 95% CI: 1.74, 3.91, p < 0.001), and that for every 1mg increase in the participants’ buprenorphine-naloxone dose, the hazard of relapse is 2% greater (HR = 1.02, 95% CI: 1.01, 1.03, p < 0.001).

Conclusion

Our analysis failed to show cannabis to be protective against relapse to opioid use in patients receiving buprenorphine-naloxone for OUD. We identified that individuals who inject drugs, are on higher doses of buprenorphine-naloxone, or have been in treatment for less than two years have a higher hazard for relapse. The presence of such factors may thus warrant closer patient follow-up and more stringent treatment protocols to mitigate risk of relapse and potential overdose.
Homicidal ideation and psychiatric comorbidities in the inpatient adolescents aged 12–17
Authors: Sun Ching-Fang , Mansuri Zeeshan , Trivedi Chintan , Vadukapuram Ramu , Reddy Abhishek
Published: November 2022

Objectives

Adolescents with a homicidal tendency is a growing concern in the United States. Studies in the past have showcased the relationship between homicidal ideation (HI) and psychiatric illnesses, but very limited information is available on the adolescent and inpatient population. We aim to evaluate the prevalence of demographic characteristics and psychiatric disorders in adolescents with and without HI.

Materials and methods

Adolescent (age 12–17) population admitted to the hospital with the diagnosis of homicidal ideation was identified from the 2016–2018 National Inpatient Sample Dataset (NISD). Patients without HI were defined as the control group. The prevalence of psychiatric comorbidities between the groups was compared by applying the Rao-Scott adjusted chi-square test. We used multivariable logistic regression to generate odds ratio (OR) of homicidal ideation as an outcome; we adjusted age, sex, race, socioeconomic status, substance use disorders, alcohol use disorders, and psychiatric comorbidities.

Results

A total of 18,935 patients (mean age: 14.5) with HI diagnosis were identified in this study. Majority of the patients were male subjects in the HI group compared to the control group (58.7 vs. 41.2%, p < 0.001). Racially, HI was more prevalent in white race (56.0 vs. 52.6%, p < 0.001) and black race (22.3 vs. 17.8%, p < 0.001), compared to Hispanic race (14.9 vs. 21.3%, p < 0.001). Major depression (Odds ratio [OR]: 2.66, p < 0.001), bipolar disorder (OR: 3.52, p < 0.001), anxiety disorder (OR: 1.85, p < 0.001), ADHD, and other conduct disorders (OR: 4.01, p < 0.001), schizophrenia (OR: 4.35, p < 0.001) are strong predictors of HI. Suicidality was prevalent in 66.9% of patients with HI.

Conclusion

We found a higher prevalence of psychiatric illnesses such as depression, anxiety, and bipolar disorder in adolescents with homicidal ideation in the inpatient setting. White and black races were more prevalent in patients with homicidal ideation. Further large-scale longitudinal research studies are warranted to establish the correlation between psychiatric disorders and homicidal ideation among adolescents.
Adherence to a Mediterranean Diet is associated with physical and cognitive health: A cross-sectional analysis of community-dwelling older Australians
Authors: Allcock Lisa , Mantzioris Evangeline , Villani Anthony
Published: November 2022

Poor cognitive function is associated with reduced functional independence, risk of institutionalization and reduced health-related quality of life. The ability to independently perform instrumental activities of daily living (iADLs) is compromised in patients with mild cognitive impairment (MCI) or dementia. Emerging evidence suggests that adherence to a Mediterranean diet (MedDiet), may play an important protective role against cognitive decline and dementia risk, whilst preserving functional status. This cross-sectional study aimed to explore the independent associations between MedDiet adherence, cognitive risk, and functional status in community-dwelling older adults living in Australia. MedDiet adherence was assessed using the Mediterranean Diet Adherence Screener (MEDAS); a modified Lawton's iADL scale was used for the assessment of functional status and risk of cognitive impairment was assessed using the AD8 dementia screening intervention. A total of n = 294 participants were included in the final analyses (70.4 ± 6.2 years; Females, n = 201; Males, n = 91; n = 2 unspecified). Adherence to a MedDiet was positively associated with functional ability (β = 0.172; CI: 0.022, 0.132; P = 0.006) independent of age, gender, Body Mass Index (BMI), smoking status, sleep duration, physical activity duration, diabetes status, and level of education. Furthermore, MedDiet adherence was inversely associated with cognitive risk (β = −0.134; CI: −0.198, −0.007; P = 0.035) independent of all covariates. However, our sensitivity analyses further showed that adherence to a MedDiet was not associated with cognitive risk in older adults free from cognitive impairment. We showed that adherence to a MedDiet is associated with healthy physical and cognitive aging. Nevertheless, exploration of these findings in larger cohorts, using longitudinal analyses and controlling for important confounders to ascertain the direction of the relationship is warranted.
The impact of facemask on patients with COPD: A systematic review and meta-analysis
Authors: Chen Xuwen , Zhang Changqing , Ibrahim Sani , Tao Shunyu , Xia Xiaoli , Li Yi , Li Caiyun , Yue Feiyan , Wang Xinhua , Bao Shisan , Fan Jingchun
Published: November 2022

Background

Since the emergence of COVID-19, mandatory facemask wearing has been implemented around the world to prevent viral transmission, however, the impact of wearing facemasks on patients with COPD was unclear.

Methods

The current study undertakes a systematic review and meta-analysis of a comprehensive literature retrieval from six databases, based on the pre-determined eligibility criteria, irrespective of language. The risk of bias was assessed using an established instrument. We primarily focused on analyzing ETCO2, SpO2, and heart and respiratory rates, and also considered the impacts on physiological and exercise performance. A descriptive summary of the data and possible meta-analysis was performed. Forest plots were generated to pool estimates based on each of the study outcomes.

Results

Of the 3,751 publications considered, six publications were selected for a systematic review and two publications were included for meta-analysis, however, the quality of these six studies was relatively low overall. In the case of inactivity, the facemask wearing COPD cohort had higher respiratory rates than that of the non-facemask wearing cohort (MD = 1.00 and 95% CI 0.47–1.53, P < 0.05). There was no significant difference in ETCO2 (MD = 0.10 and 95% CI −1.57–1.78, P > 0.05) and heart rate (MD = 0.40 and 95% CI −3.59–4.39, P > 0.05) nor SpO2 (MD = −0.40 and 95% CI −0.84–0.04, P > 0.05) between the COPD patients with and without facemasks. Furthermore, it was observed that the only significant differences between the COPD patients with and without facemasks undertaking different activities were FEV1 (%) (MD = 3.84 and 95% CI 0.14–7.54, P < 0.05), FEV1/FVC (%) (MD = 3.25 and 95% CI 0.71–5.79, P < 0.05), and blood lactate (MD = −0.90 and 95% CI −1.73 to −0.07, P < 0.05).

Conclusion

Wearing facemasks decreased the exercise performance of patients with COPD, however, it had minimal impact on physiological indexes. Further investigations will be performed on the high-quality data from randomized control studies.

Systematic review registration

https://www.crd.york.ac.uk/PROSPERO/display_record.php?RecordID=326265, identifier: CRD42022326265.