bluuebunny/crossref_metadata_2025 huggingface dataset:
Fin Type Variation of Lionhead Strain Goldfish (Carassius auratus) Offspring
Authors: Syaifudin M. , Carman Odang , Sumantadinata K.
Published: February 2017

 

Lionhead strain goldfish (Carassius auratus) inheritance produce many variations in phenotype qualitative traits of their offspring that is not common with the parents. Lionhead is an ornamental freshwater fish, they do not have a dorsal fin, but it is a beauty finfish, is popular to the people and have a high economic value. Of the 846 offspring of lionhead is produced in this experiment, and 57,7% of them have dorsal fin (42,3% normal), 13,1% of them have anal fin which did not similar with their parents, 58,6% caudal fin of them did not have similar to their parents. It might be caused by incompletely segregation in meiosis and many gen responsible to certain phenotype trait. Another abnormalities such as no anal fin, blindness, stumped and no pigmen in their gill lamella also occurred.

Key words: Inheritance, fin, phenotype, abnormality

 

ABSTRAK

Ikan maskoki strain lionhead menghasilkan keturunan dengan fenotip yang sangat bervariasi dan berbeda dengan induknya. Ikan ini merupakan ikan hias air tawar yang tidak memiliki sirip punggung namun tetap memiliki keindahan, sehingga menjadi begitu populer di masyarakat dan memiliki nilai ekonomi yang tinggi. Sebanyak 846 keturunan (anakan) telah dihasilkan dalam percobaan ini, dan sebanyak 57,7% dari populasi tersebut memiliki sirip punggung (berarti 42,3% merupakan keturunan normal), 13,1% memiliki sirip dubur yang tidak mirip dengan induknya. Variasi keturunan ikan maskoki strain lionhead ini disebabkan oleh segregasi yang tidak sempurna dalam proses meiosis dan banyaknya gen yang terlibat dalam pembentukan penotip tertentu. Abnormalitas lainnya juga terjadi pada keturunan ikan maskoki strain lionhead ini, seperti tidak adanya sirip dubur, mata buta, tubuh pendek dan tidak adanya pigmen pada lemela insang
 Kata kunci: Keturunan, sirip, fenotip, abnormalitas

 
Pengaruh Naupli Artemia yang Diperkaya dengan N-3 HUFA terhadap "Budget" Energi Larva Ikan Red Sea Bream Pagrus major
Authors: Sumule O.
Published: February 2017

Energy budget of red sea bream Pagrus major larvae fed n-3 highly unsaturated fatty acids (HUFA)-enriched (EA) and non-enriched (NEA) Artemia nauplii was constructed as: EI = F + M + U + G, where EI is energy intake, F energy loss as feces, M energy loss for metabolism, U energy loss as non-fecal matter based on ammonia excretion, and G energy for growth. Larvae (29 days post hatching, 41,1 mg mean wet weight) were reared in six 80 1 circular tanks and fed EA and NEA for 12 days, with three replicates for each type of food. Overall, growth of larvae was significantly higher in EA group than NEA group. Oxygen consumption, as heat increment, was also significantly higher in EA-fed larvae than NEA-fed larvae. The energy budget of a 100-mg larva was partitioned into: 100% of EI = 38,4% for G + 34,5% for M + 2,9% for U + 24,2% for F, for EA group, whereas 100% of EI = 29,1% for G + 30,1% for M + 3,3% for U + 37.4% for F, for NEA group. Assimilation, gross conversion, and net conversion efficiencies were higher in EA-fed larvae than NEA-fed larvae, which were attributed to the higher energies channeled to metabolism and growth in the former. This study concluded that HUFA enrichment of Artemia nauplii increased energy absorption but reduced energy excretion in red sea bream larvae.
 Key words : Pagrus major, larvae. HUFA enriched Artemia nauplii, oxygen consumption, ammonia excretion, heat increment, energy budget
Intensity and Prevalence of Ectoparasites in Common Carp (Cyprinus carpio) fry from Traditional Pond and “Longyam” at Sukamulya Villages, Singaparna, Tasikmalaya
Authors: Rustikawati I. , Rostika R. , Iriana D. , Herlina E.
Published: February 2017

The experiment was done to find out species, intensity and prevalence of common crap fry from traditional ponds and "longyam" at Sukamulya village, Singaparna, Tasikmalaya. This experiment was conducted at Sukamulya village and Aquatic Biology Laboratory of Fisheries Department, Faculty of Agriculture, Padjajaran University form October 1st 2002 to October 31th 2002. Samples of common carp fry were obtained from six traditional ponds and six "longyam". The survey method was used in this experiment of common carp fry at difference type of pond were based on the Wilcoxon rank sum test. Intensity and prevalence of ectoparasites of common carp fry at traditional ponds and longyam was based on the Kruskal-Wallis test. The result of this experiment showed that eight genera of ectoparasites were found, consisted of three Platyhelminthes: Dactylogyrus (47,01%), Gryrodactylus (3,84%), Transversotrema (0,01%), four Protozoa: Ichtyophthrius (9,59%), Epistylis (1,05%), Trichodina (1,68%) and one Arthopoda: Acarus (0,01%), The intensity of Dactylogyrus was 28,83. Gryrodactylus 4.98, Transversotrema 0,17, Ichthyophthirius 13,51, Epistylis 4,68, Trichodina 25,14 Trichodinella 6,55 and Acarus 0,17. The prevalence of Dactylogyrus is 90,00% Gyrodactylus 44,82%, Transversotrema 0,37%, Ichthyophthirius 42,17, Epistylis 13,17%, Trichodina 82,59%, Trichodinella 17,04 and Acarus 0,37%.

Key words: Intensity, prevalence, ectoparasites, common carp fry, traditional pond, "longyam"
Effect of Adding the White Legged Shrimp (Penaeus vannamei) on Growth and Survival of Tiger Shrimp (Penaeus monodon) in Intensive Farming
Authors: Tarsim .
Published: February 2017

This experiment was carried out in the framework of a project to develop a viable shrimp polyculture technology under intensive farming. The objectives were to assess the effect of adding white legged shrimp (Penaeus vannamei) on growth and survival of tiger shrimp (Penaeus monodon) in intensive Farming. Tiger shrimp were stocked in 2800-3400 m2 earthen ponds at 40 individuals/m2 and reared for 133 days. White legged shrimp added at 70 day at 8±2 individuals/m2. Growth rate and total yield affected by addition of this species. Final  affected by white legged shrimp addition. No adding effects were found on the water quality parameters. The results indicate that, at densities tested, white legged shrimp addition to the intensive farming of tiger shrimp polyculture is viable as species increase crop production

Key words: Penaeus vannamei, P. monodon, growth, survival, polyculture
White Shrimp (Litopenaeus vannamei) Production on Different Rearing Densities in Biocrete Pond
Authors: Budiardi T. , Muzaki A. , Utomo N.B.P.
Published: February 2017

Study on white shrimp (Litopenaeus vannamei) production in biocrete pond in different density was performed at PT. Bimasena Segara, Citarate, Sukabumi, West Java. Six ponds were used and devided into 2 groups based the density levels, i.e., 72-73 shrimp/m2 and 93-105 shrimp/m2.  Experiment was done for 100 days, including pond preparation.  The results of study showed that mean weight of shrimp reared in biocrete pond was ranged from 9.64 to 12.34 gram, survival rate 26.97-99.58%, productivity 3,078-8,340.9 kg/ha, feed quantity 2,084.5-2,870.4 kg and FCR 1.19-2.60.  Ponds with lower rearing density had lower (P<0.1) productivity, mean weight, survival and FCR compared to those of higher rearing density.

Keywords: Litopenaeus vannamei, biocrete pond, rearing density

 

ABSTRAK

Penelitian mengenai produksi udang vaname (Litopenaeus vannamei) di tambak biocrete dengan padat penebaran berbeda telah dilakukan di tambak PT. Bimasena Segara, Citarate, Sukabumi, Jawa Barat menggunakan 6 petak tambak yang dibedakan menjadi 2 kelompok berdasarkan padat tebar udang yang selanjutnya menjadi perlakuan, yaitu : 72-73 ekor/m2 dan 93-105 ekor/m2. Penelitian ini dilakukan selama 100 hari termasuk persiapan tambak.  Hasil penelitian menunjukkan abhwa bobot rata-rata udang yang dipelihara di tambak biocrete berkisar antara 9,64-12,34 g, kelangsungan hidup berkisar antara 26,97-99,58%, produktivitas berkisar antara 3078,7-8340,9 kg/ha, jumlah pakan yang diberikan berkisar antara 2084,5-2870,4 kg dan FCR berkisar antara 1,19-2,60. Petak dengan kepadatan rendah memiliki nilai produktivitas, bobot rata-rata, kelangsungan hidup dan konversi pakan (FCR) berbeda nyata (P < 0,1) dan lebih baik dibanding dengan yang berkepadatan tinggi.

Kata kunci : Litopenaeus vannamei, tambak biocrete, padat tebar
Potential of Chromolaena odorata Leaf as A Cure of Aeromonas hydrophila on Giant Gouramy (Osphronemus gouramy)
Authors: Hadiroseyani Y. , Hafifuddin . , Alifuddin M. , Supriyadi H.
Published: February 2017

This study was conducted to examine the potency of Chromolaena odorata leaf extract as a medicine for skin eruption disease caused by  Aeromonas hydrophila in giant gouramy Osphronemus gouramy.  Leaf extract of Chromolaena odorata for in vitro test was 0 (as control), 13000, 15000, 17000, 19000 and 21000 ppm, poured onto TSA medium containing bacteria 103 cfu/ml, and then is incubated for 24 hours. In vivo test was performed by injecting bacteria 0.1 ml of 109 cfu/ml intramuscularly into giant gouramy (14 g weight), and then  fish were maintained in the water containing 15000 ppm of Chromolaena odorata leaf extract. In vitro study showed that prevention area of leaf extract against Aeromonas hydrophila was increase by increasing the concentration of leaf extract used, reached 9,33 mm.  Prevention zone of leaf extract by difusion tends to constant, reached 7,6 mm. By in vivo test, survival rate of giant gouramy infected by Aeromonas hydrophila was no significantly different between dosages of leaf extract.  All treated fish, excluded control died after 24 hours infection.

Keywords: Aeromonas hydrophila, Osphronemus gouramy, Chromolaena odorata

 

ABSTRAK

Penelitian ini dilakukan untuk mengetahui potensi daun kirinyuh Chromolaena odorata sebagai obat untuk penyakit cacar yang diakibatkan oleh Aeromonas hydrophila pada ikan gurame Osphronemus gouramy. Konsentrasi ekstrak daun Chromolaena odorata untuk uji in vitro adalah 13000, 15000, 17000, 19000 dan 21000 serta 0 ppm sebagai kontrol, yang diletakkan di atas media TSA yang telah mengandung biakan bakteri 103 cfu/ml dan diinkubasi selama 24 jam. Uji in vivo dilakukan dengan menginjeksikan bakteri  sebanyak 0,1 ml (109 cfu/ml) secara intramuskular ke ikan gurame (berat 14 g) dan kemudian ikan dipelihara dalam air yang mengandung ekstrak daun kirinyuh 15000 ppm. Hasil uji in virto menunjukkan bahwa semakin tinggi konsentrasi ekstrak daun kirinyuh basah semakin efektif dalam menghambat perkembangan A. hydrophila dengan zona hambat tertinggi mencapai 9,33 mm. Zona hambat yang dihasilkan melalui metode difusi cenderung konstan, mencapai 7,6 mm. Melalui uji in vivo, tingkat kelangsungan hidup ikan gurame yang tidak berbeda nyata pada masing-masing perlakuan, bahkan terjadi kematian total dalam 24 jam pada semua perlakuan, kecuali kontrol.

Kata kunci: Aeromonas hydrophila, Osphronemus gouramy, Chromolaena odorata
Quality of sperm from cryopreserved semen of Tor soro in Dimethylsulfoxide and Glycerol 5, 10 and 15%
Authors: Junior M. Zairin , Handayani S. , Supriatna I.
Published: February 2017

Attempt to produce a lot number of Tor soro is difficult if only obtained by natural spawning since they often get mature in dissimilar time. Stock of sperm kept in enough number for fertilization in anytime is needed to overcome the problem.  Cryopreservation of semen is one of sperm cryopreservation methods using liquid nitrogen (-196oC) to quickly freeze the sperm. Several cryoprotectants can be utilized in cryopreservation.  In this study, quality by means of motility of cryopreserved sperm using dimethylsulfoxide or glycerol as cryoprotectant in the dose of 5, 10 and 15% were analyzed. The result of study show that percentage of sperm motile after cryopreservation using dimethylsulfoxide and glycerol was similar ranged from 76.7 to 83.3%.  Therefore, both cryoprotectant used in this study could be employed for cryopreservation of Tor soro sperm.

Keywords: cryopreservation, dimethylsulfoxide, glycerol, sperm, Tor soro

 

ABSTRAK

Upaya produksi ikan batak (Tor soro) dalam jumlah besar relatif sulit jika hanya mengandalkan hasil pemijahan secara alami karena kematangan gonad antara ikan jantan dan betina sering tidak bersamaan. Untuk mengatasinya diperlukan stok sperma yang disimpan dalam jumlah yang cukup banyak untuk pembuahan yang dapat dipakai setiap saat. Kriopreservasi semen merupakan salah satu cara penyimpanan sperma yang menggunakan larutan nitrogen cair (-196°C) untuk membekukan sperma secara cepat. Beberapa jenis protektan dapat digunakan dalam pembekuan sperma. Pada penelitian ini, dilakukan analisis kualitas dalam arti motilitas sperma hasil kriopreservasi menggunakan dimetilsulfoksida dan gliserol sebagai krioprotektan dengan dosis 5, 10 dan 15%.  Hasil penelitian menunjukkan bahwa persentase sperma motil setelah dilakukan kriopreservasi adalah sama, berkisar antara 76,7% hingga 83,35%.  Dengan demikian, kedua jenis krioprotektan yang digunakan dalam penelitian ini bisa dipakai dalam kriopreservasi sperma ikan batak.

Kata kunci: kriopreservasi, dimetilsulfoksida, gliserol, sperma, Tor soro
Effect injection of Ginger Extract on Development and Nucleus position of “Sangkuriang” Catfish Clarias sp. eggs
Authors: Junior M. Zairin , Yustikasari Y. , Arfah H.
Published: February 2017

One of methods could be applied to continuously meet the need of fish fry including catfish (Clarias sp.) is artificial propagation by inducing ovulation and spawning.   As an alternative of existing method, in this study, ginger extract was intramuscularly injected to induce development of catfish eggs.  Ginger is known as an important regulator of the balance of arachidonat cycle.  The dose of ginger extract injected was 0, 0.5, 1.0 and 1.5 mL/kg broodstock.  The results of study showed that injection 100% of ginger extracts in all doses was insignificantly inducing development of egg diameter and its nucleus position of catfish.  Other chemicals exist in ginger extract might be functions as an obstacle for egg development of catfish.

Keywords: catfish, Clarias sp., ginger, ovulation, egg

 

ABSTRAK

Salah satu cara untuk memenuhi kebutuhan benih ikan termasuk lele (Clarias sp.) secara kontinyu adalah penggunaan teknologi pembiakan buatan melalui perangsangan ovulasi dan pemijahan. Sebagai alternatif teknik yang sudah ada, dicobakan perangsangan perkembangan telur ikan lele menggunakan bahan ekstrak jahe yang dilakukan melalui penyuntikan secara intramuskular. Jahe telah dikenal sebagai suatu pengatur penting atas keseimbangan siklus arakidonat. Dosis ekstrak jahe yang disuntikkan adalah 0, 0,5, 1 dan 1,5 mL/kg induk. Penyuntikan 100% ekstrak jahe pada semua dosis perlakuan belum dapat merangsang perkembangan diameter dan posisi inti sel telur ikan lele sangkuriang. Adanya bahan lain yang terdapat pada jahe diduga sebagai penghambat bagi perkembangan telur ikan lele.

Kata kunci: Ikan lele, Clarias sp., jahe, ovulasi,  sel telur
Effect of Ultraviolet Radiation on Pathogenity of White Spot Syndrome Virus in Giant Tiger Prawn (Penaeus monodon Fab.)
Authors: Subkhan M. , Alifuddin M. , Taslihan A.
Published: February 2017

This experiment was conducted to examine the effect of UV irradiation on pathogenity of white spot syndrome virus (WSSV) in black tiger shrimp (Penaeus monodon Fab.).  A hundred ml of WSSV virus suspension (200 μg/ml) were placed at 30 cm under UV light 10 Watt.  Radiation on WSSV virus was performed for 15, 30, 45, and 60 minutes.  Black tiger sjrimp in density of 260 tails/L were immersed in 1000 ml of irradiated virus suspension (20 μg/ml) to test their pathogenities.  The results of study showed that duration of UV irradiation on WSSV virus was reverse correlated to their pathogenities.  In constrast, survival of black tiger shrimp was linear correlated to duration of UV inactivation of virus.  Higher survival rate of shrimp (65.52%) after challenge test was obtained by irradiation of virus for 60 min.

Keywords:  WSSV, virus, pathogen, ultraviolet, black tiger prawn, Penaeus monodon

 

ABSTRAK

Penelitian ini dilakukan untuk mengetahui pengaruh radiasi UV terhadap patogenitas virus White Spot Syndrome Virus (WSSV) pada udang windu (Penaeus monodon Fab).   Suspensi virus WSSV dengan konsentrasi 200 μg/ml sebanyak 100 ml ditempatkan 30 cm diradiasi menggunakan UV 10 Watt.  Radiasi dilakukan selama 15, 30, 45, dan 60 menit.  Udang dengan kepadatan 260 ekor/L direndam dalam 1000 ml suspensi virus (20 μg/ml) hasil radiasi untuk menguji patogenitasnya.  Hasil penelitian menunjukkan bahwa lama radiasi UV pada virus WSSV berbanding terbalik dengan tingkat patogenitasnya.  Sementara itu, lama inaktivasi virus dengan UV berbanding lurus terhadap kelangsungan hidup udang.  Kelangsungan hidup udang windu tertinggi (65,52%) setelah uji tantang diperoleh dengan meradiasi virus selama 60 menit.

Kata kunci: WSSV, virus, patogen, ultraviolet, udang windu,  Penaeus monodon
Oxygen Consumption of White Shrimp (Litopenaeus vannamei) and Model of Oxygen Management in Intensive Culture Pond
Authors: Budiardi T. , Batara T. , Wahjuningrum D.
Published: February 2017

This study was conducted to determine oxygen consumption level of white shrimp (Litopenaeus vannamei) and the model of oxygen management in intensive culture pond.   Shrimp in weight of 5 gram were maintained in 20 liter of water in density 6 tails/container.  Water quality was measured every 2 hours for 6 hours.  Other experiments were done using shrimp in weight of 8, 10, 12 and 15 gram per tail, in density 4 tails/container.  The results of study showed that oxygen consumption levels of white shrimp was higher after feeding than before feeding.  Oxygen consumption levels of smaller shrimp were higher than that of bigger shrimp.  Regression model of oxygen consumption levels before and after feeding were linear. Correlation between oxygen consumption and shrimp weight reached 92.5%. 

Keywords: white shrimp, Litopenaeus vannamei, oxygen, respiration, intensive pond

 

ABSTRAK

Penelitian ini dilakukan untuk mengetahui tingkat konsumsi oksigen udang vaname (Litopenaeus vannamei) dan model pengelolaan oksigen pada tambak intensif.  Udang dengan bobot sekitar 5 gram dimasukkan ke dalam wadah tertutup berukuran 20 liter dengan kepadatan 6 ekor/wadah.  Kualitas air diukur setiap 2 jam selama 6 jam. Perlakuan tersebut juga dilakukan pada udang dengan kelompok berat 8, 10, 12 dan 15 gram, dengan kepadatan 4 ekor/wadah.  Hasil penelitian menunjukkan bahwa tingkat konsumsi oksigen udang vaname sesudah makan relatif lebih tinggi daripada sebelumnya.  Tingkat konsumsi oksigen udang ukuran kecil relatif lebih tinggi daripada yang berukuran lebih besar. Model persamaan regresi tingkat konsumsi oksigen pada udang yang tepat sebelum dan sesudah makan adalah linear. Korelasi antara konsumsi oksigen dengan bobot udang mencapai 92,5%.  

Kata kunci: vaname, Litopenaeus vannamei, oksigen, respirasi, tambak intensif
Effect of L-Ascorbyl-2-Phosphate Magnesium as a Vitamin C Source in Different Doses on Growth of Patin Pangasius Hypophthalmus Fingerlings
Authors: Jusadi D. , Dewantara B.A. , Mokoginta I.
Published: February 2017

This study was aimed to determine optimum dose of L-Ascorbyl-2-Phosphate Magnesium in the diet of patin, Pangasius hypophthalmus fingerlings.  Five isoprotein and isocaloric diets containing different dose of L-Ascorbyl-2-Phosphate Magnesium, i.e. 0, 25, 75 and 100 mg/kg diet were used in this experiment.  Fish with an average size of 5.00±0.11 g were maintained at a density of 15 fish per aquarium.  Fish were fed three times a day at satiation, for 40 days.  The results of study shows that vitamin C content in fish body increased as the vitamin C level of the diet increased.  Similar pattern to vitamin C content was also found in protein retention, lipid retention, daily growth rate, and feed efficiency (p< 0.05).  Thus, it can  be concluded that the most optimum dose of L-Ascorbyl-2-Phosphate Magnesium as vitamin C source for patin fingerlings in this experiment was 100 mg Vit C/kg diet. Higher dose of L-Ascorbyl-2-Phosphate Magnesium than 100 mg/kg diet however need to be further verified.

Keywords: vitamin C, patin, Pangasius hypophthalmus.

 

ABSTRAK

Penelitian ini bertujuan untuk menentukan kadar optimum L-Ascorbyl-2-Phosphate Magnesium dalam pakan ikan patin, Pangasius hypophthalmus ukuran sejari. Lima macam pakan isoprotein dan isokalori mempunyai kandungan L-Ascorbyl-2-Phosphate Magnesium berbeda, berturut-turut 0, 25, 50, 75 dan 100 mg/kg pakan telah digunakan dalam penelitian ini. Benih berukuran rata-rata 5,00±0,11 g dipelihara dalam akuarium dengan kepadatan 15 ekor per akuarium. Ikan diberi pakan tiga kali sehari, secara at satiation, selama 40 hari. Hasil penelitian menunjukkan bahwa kadar vitamin C tubuh meningkat sejalan dengan kadar vitamin C pakan. Demikian pula retensi protein, retensi lemak laju pertumbuhan harian, efisiensi pakan mengikuti pola yang sama seperti kandungan vitamin C tubuh (p<0,05). Dengan demikian dapat disimpulkan bahwa kadar vitamin C L-Ascorbyl-2-Phosphate Magnesium terbaik dalam pakan ikan patin adalah 100 mg/kg pakan, namun perlu diteliti lebih lanjut kadar L-Ascorbyl-2 Phosphate Magnesium yang lebih tinggi dari 100 mg/kg pakan.

Kata kunci: vitamin C, ikan patin, Pangasius hypophthalmus.
Effect of Feeding Diet Containing Different Dose of Vitamin E with 1:2 of n-3/n-6 Fatty Acid Content on Reproductive Performance of Female Zebrafish Danio rerio
Authors: Utomo N.B.P , Nurjanah N. , Setiawati Mia
Published: February 2017

The role of essential fatty acids  as a precursor of prostaglandin and as a component of cell membrane in the form of phospholipids, can affect cell fluidity. Vitamin E functions as  fatty acid antioxidant in the body and affects egg quality.  Vitamin E and essential fatty acids (n-3 and n-6) are required at the same time in a proper  ratio to induce fish gonad maturation.  Zebra fish Danio rerio broodstock in 29 days pre-saline were fed on diet containing 4 different vitamin E concentration; i.e. 325, 375, 425 and 475 mg/kg diet with constant n-3 and n-6 fatty acid doses of 1% and 2%, respectively, for 6 weeks.  Reproduction performance in terms of fecundity was improved (p<0.05), whilst gonado somatic index, gonado somatic index saline, fertilization rate, hatching rate, survival rate, yolk absorption rate and daily growth rate were insignificantly different.

Keywords: vitamin E, fatty acid, reproduction performance, Danio rerio
   ABSTRAK
Asam lemak esensial berperan sebagai prekursor prostaglandin dan sebagai fosfolipid pada membran sel yang dapat mempengaruhi fluiditasnya. Sedangkan vitamin E berperan  sebagai antioksidan asam lemak dalam tubuh sehingga berpengaruh terhadap kualitas telur yang dihasilkan. Vitamin E dan asam lemak  essensial (n-3 dan n-6) dibutuhkan secara bersamaan dengan kombinasi perbandingan yang tepat untuk pematangan gonad ikan. Induk ikan yang digunakan dalam penelitian ini adalah ikan zebra Danio rerio yang berumur 29 hari pra salin dan dipelihara selama 6 minggu dengan pakan mengandung kadar vitamin E yang berbeda yaitu 325, 375, 425, dan 475 mg/kg pakan pada kadar asam lemak n-3 (1%) dan n-6 (2 %) tetap. Penampilan reproduksi pra salin ikan tersebut hanya berpengaruh nyata terhadap fekunditas namun tidak berpengaruh nyata terhadap gonado somatik indek, gonado somatik indek salin, derajat pembuahan telur, derajat tetas telur, kelangsungan hidup larva, laju penyerapan kuning telur dan laju pertumbuhan harian.

Kata kunci: Vitamin E, asam lemak, penampilan reproduksi, Danio rerio
Development of Digestive Enzyme of Patin Pangasius hypohthalmus Larvae
Authors: Effendi I. , Augustine D. , Widanarni .
Published: February 2017

Culture of patin Pangasius hypophthalmus especially larval rearing  very depends on the supply of natural food as energy source.  Artemia is the main natural food for fish larvae as a starter food, but its price is high.  To reduce production cost, farmers tend to reduce the feeding frequency and shorten  the Artemia feeding period.  Altering feeding regime however may reduce fry quality. This relate to the availability of digestive enzymes.  The objective of this study was  to examine digestive enzymes activity in patin larvae fed with  different feeding regime.  By shorten feeding period with Artemia to 2-4 days and Tubifex,substitution, the enzymes activity of protease, lipase and amylase were revealed similar pattern   The enzymes activity tends to increase and reach the peak at day 7 , and decrease later on until day 15 after hatching.  Survival rate of fish were varied for each treatment, and the highest survival rate was obtained when larvae were fed by Artemia for 8 days.  Blood worm were not fully digested by patin larvae at early stage.

Keywords: enzyme, digestion, patin, Pangasius hypophthalmus

 

ABSTRAK

Proses budidaya ikan patin, Pangasius hypophthalmus terutama pembenihan sangat tergantung oleh ketersediaan pakan alami sebagai sumber energinya. Artemia merupakan pakan alami yang banyak diberikan pada saat larva ikan mulai makan, namun harganya relatif tinggi. Untuk menekan biaya produksi, petani ikan patin cenderung mengurangi frekuensi pemberian Artemia dan mempersingkat waktu pemberiannya. Penggeseran jadwal ini diduga mengakibatkan penurunan kualitas benih ikan patin yang dihasilkan yang berhubungan dengan kesiapan enzim pencernaannya. Penelitian ini dilakukan untuk mengetahui aktivitas enzim pada larva ikan patin dengan jadwal pemberian pakan yang berbeda. Dengan memotong waktu pemberian Artemia 2 - 4 hari dan disubstitusi dengan Tubifex, aktifitas enzim protease, lipase dan amilase pada larva ikan patin, memiliki pola yang sama. Aktifitas enzim cenderung meningkat dan mencapai puncak pada umur 7 hari, selanjutnya terus menurun sampai larva berumur 15 hari. Kelangsungan hidup ikan selama penelitian berbeda-beda untuk setiap perlakuan dengan nilai tertinggi dicapai oleh larva yang diberi pakan Artemia sampai berumur 8 hari. Larva ikan patin belum siap sepenuhnya untuk menerima pakan berupa cacing sutera sejak stadia awalnya.

Kata kunci: Enzim, Pencernaan, Patin, Pangasius hypophthalmus
Effect of Enriched Feed by Immunostimulant on Immune Response and Growth of Humpback Grouper, Cromileptes altivelis
Authors: Suprayudi M. Agus , Indriastuti L. , Setiawati Mia
Published: February 2017

Humpback grouper, Cromileptes altivelis is susceptible to low quality of environmental condition so that it is vulnerable to bacterial infection.  One of the methods to solve this problem is improvement of nutrition by using immune response present in fish body.  Supplementation of immunostimulant in the diet may increase immune response of humpback grouper regarding to unsuitable environment condition and bacterial infection.  Humpback grouper in average weight of 24.6 ± 0.05 g were reared in a density of 10 fish per aquarium for 34 days.  Diet was supplemented with 4 g yeast per kg diet (diet A), 0.1 g vitamin C per kg diet (diet B), 2.5 g β-glucan (diet C), chromium-yeast 0,015 g/kg diet (diet D) and no supplementation (diet E) as control. Blood performance was analyzed after challenging test with Vibrio parahaemolyticus. The result of study indicated that immunostimulant supplementation increased leucocytes population during salinity stress test. Supplementation of chromium-yeast in the diet increased resistant of humpback grouper to Vibrio parahaemolyticus infection.

Keywords: immunostimulant, humpback grouper, Cromileptes altivelis

 

ABSTRAK

Ikan kerapu bebek, Cromileptes altivelis sangat rentan dengan kondisi lingkungan yang buruk sehingga mudah terinfeksi bakteri. Salah satu cara untuk mengatasi masalah tersebut adalah perbaikan nutrisi dengan memanfaatkan respon imun yang terdapat pada tubuh ikan. Penambahan bahan-bahan imunostimulan pada formulasi pakan buatan diharapkan dapat meningkatkan respon imunitas ikan kerapu bebek terhadap lingkungan yang tidak mendukung dan infeksi bakteri. Ikan kerapu bebek dengan bobot rata-rata 24,6 ± 0,05 g dipelihara dengan kepadatan 10 ekor/akuarium selama 34 hari. Pakan yang diberikan berupa pelet dengan tambahan beberapa bahan imunostimulan yaitu 4 g/kg ragi komersial (pakan A), 0,1 g/kg vitamin C (pakan B), 2,5 g/kg β-glukan (pakan C), kromium-yeast 0,015 g/kg (pakan D) dan tanpa penambahan bahan (pakan E) sebagai pembanding. Gambaran darah dianalisis setelah dilakukan uji tantang dengan Vibrio parahaemolyticus. Hasil penelitian menunjukkan bahwa penambahan bahan-bahan imunostimulan tersebut ternyata mampu meningkatkan populasi leukosit selama uji stres salinitas. Pemberian kromium-yeast dalam pakan meningkatkan daya tahan ikan kerapu bebek terhadap serangan Vibrio parahaemolyticus.

Kata kunci: immunostimulan, kerapu bebek, Cromileptes altivelis
Prospect use of Phaleria macrocarpa to prevent motile aeromonad septicaemia disease in Patin Catfish Pangasianodon hypophthalmus
Authors: Wahjuningrum D. , Angka S.L. , Lesmanawati W. , Sa’diyah . , Yuhana M.
Published: February 2017

Motile Aeromonad Septicaemia (MAS) disease is one of bacterial disease frequently infecting freshwater fishes including patin catfish Pangasianodon hypophthalmus.  This study was performed to determine antimicrobial of Phaleria macrocarpa (PM) and its potency against MAS disease caused by Aeromonas hydrophila.  The in vitro susceptibility test was performed by pour plate methods at the dosages of 2, 4, 6, 8, and 10 g/l PM. At the in vivo test, fish were fed with the addition of PM into the diet at a dosage of 6, 12, and 18 g/l and 0 g/l as a control for 8 days. At ninth day, fish were infected with A.hydrophila. For seven days after infection the clinical signs and blood pictures were observed. The in vitro test indicated that PM had an antibacterial effect to A.hydrophila at the dosage of 6 g/l. Addition of PM in the diet for 8 days increased haemoglobine. The results showed that lowest clinical sign and smallest number of in fected fish was found at dosage of 12 g/l PM. PM can be used as a preventive method for MAS.

Keywords:  Phaleria macrocarpa, antibacterial, "patin", MAS disease, Aeromonas hydrophila

 

Abstrak

Penyakit MAS (Motile Aeromonad Septicaemia) merupakan penyakit bakterial yang banyak menyerang ikan-ikan air tawar termasuk patin Pangasianodon hypophthalmus. Penelitian ini bertujuan untuk melihat kemampuan antibakteri dari mahkota dewa (MD) Phaleria macrocarpa terhadap Aeromonas hydrophila penyebab penyakit MAS dan potensinya dalam pencegahan penyakit ini.  Pada uji in vitro dilakukan pengujian aktivitas antibakteri MD terhadap A. hydrophila dengan metode hitungan cawan pada dosis MD 2, 4, 6, 8, dan 10 g/l. Pada uji in vivo, ikan uji diberi pakan yang dicampur MD dengan dosis berbeda yaitu 0 g/l (kontrol +), 6, 12, dan 18 g/l, selama 8 hari. Pada hari kesembilan ikan disuntik dengan A. hydrophila dan pengamatan dilanjutkan selama 7 hari, meliputi pengamatan gejala klinis dan gambaran darah.  Hasil penelitian menunjukkan bahwa MD bersifat antibakteri terhadap A. hydrophila dengan dosis efektif 6 g/l. Pemberian MD selama 8 hari dapat meningkatkan kadar hemoglobin, kadar hematokrit, jumlah lekosit, serta meningkatkan kemampuan fagositik darah. Dosis MD sebesar 12 g/l menunjukkan hasil paling baik yang ditunjang oleh gejala klinis paling ringan (sampai tahap nekrosis), dengan jumlah ikan yang terinfeksi paling sedikit (45%) dan waktu penyembuhan paling cepat (hari ke 6). Dengan demikian, MD dapat digunakan untuk mencegah penyakit MAS.

Kata kunci:  mahkota dewa, antibakterial, ikan patin, penyakit MAS, Aeromonas hydrophila
Dietary Zinc Requirement of Young Giant Gouramy (Osphronemus gouramy, Lac.)
Authors: Setiawati Mia , Azwar N.R. , Mokoginta I. , Affandi R.
Published: February 2017

This experiment was conducted to determine the dietary zinc requirement of young giant gouramy. Five experimental diets were used in this experiment containing iso-nitrogenous and iso-energy with different levels of zinc (0, 25, 50, 100, and 200 mg per kg). The initial means of body weight of body the fishes was 0.82 g per individual. The fishes were fed upon the diets at satiation, three times daily, for 12 weeks. The results indicated no zinc deficiency symptoms in this experiment. The daily growth rate, feed efficiency, survival rates and protein and lipid retention were not significantly different from all the diets. However, fish fed upon 25 mg Zn/kg diets produced the highest protein and total digestibility. The zinc contents of 25 mg Zn/kg diets gave the highest value in zinc accumulation in the bone, blood serum and eyes. The highest accumulation of the zinc in the liver occurred in 50 mg Zn/kg diets. There was increase in zinc content of the tissues after increasing levels of dietary zinc. Thus, Zn requirement for juvenile of giant gouramy was 25-50 mg/kg diets.

Keywords: Osphronemus gouramy, Zinc, diet

 

ABSTRAK

Penelitian ini dilakukan untuk mengetahui kebutuhan seng dalam pakan untuk benih ikan gurame.  Lima jenis pakan percobaan yang diuji mengandung iso-nitrogen dan iso-energi dengan kadar seng (Zn) yang berbeda (0, 25, 50, 100, and 200 mg per kg).  Bobot rata-rata benih yang digunakan adalah 0,82 g per ekor.  Ikan dipelihara selama 12 minggu dan diberi pakan 3 kali sehari.  Hasil penelitian menunjukkan bahwa tidak ada gejala defisiensi Zn.  Laju pertumbuhan harian, efisiensi pakan, kelangsungan hidup dan rentesi protein dan lemak tidak berbeda nyata untuk semua perlakuan.  Namun demikian, ikan yang diberi Zn sebanyak 25 mg/kg pakan menghasilkan kecernaan total dan protein tertinggi.  Perlakuan tersebut juga menghasilkan akumulasi Zn tertinggi dalam tulang, serum darah dan mata.  Akumulasi Zn tertinggi dalam hati diperoleh pada perlakuan 50 mg Zn/kg pakan.  Kandungan Zn dalam jaringan meningkat sejalan dengan meningkatnya kadar Zn dalam pakan. Dengan demikian, kebutuhan Zn bagi benih ikan gurame adalah sebanyak 25-50 mg /kg pakan.
 Kata kunci: Osphronemus gouramy, seng, pakan
Preliminary study on domestication of coconut crab (Birgus latro)
Authors: Sulistiono . , Refiani S. , Tantu F.Y. , . Muslihuddin
Published: February 2017

Preliminary study on domestication of coconut crab (Birgus latro) had been done since June - November 2004 and December - 2005 February 2006.  Study was carried out in two locations namely Laboratory of Sekolah Tinggi Kelautan dan Perikanan, Palu to study adult crab, and Laboratory of Ecobiology of Faculty of Fisheries and Marine Science, Bogor Agricultural University to study crab in clomang stage.  Adult crabs were collected by using some fishing gears namely trap, net and directly using bamboo stick.  Domestication was done in rearing batch sized 1x1x1 m3 with artificial nests (30x15x15 cm3), and small batch for water (7x10x10 cm3).  While for clomang stage, crabs were reared in aquarium (80x40x40 cm3) with debris and small water batch.  Result showed that adult crab had a survival rate around 12.5-0%, and mortality around 50-87.5%.  A similar result was also in clomang stage, which was 12.5% for survival rate and 87.5% for mortality.  Adult coconut crab was preferred to feed coconut than other food likely vegetable and chick.

Keywords: domestication, coconut crab, Birgus latro, food habit

 

ABSTRAK

Penelitian awal mengenai kajian awal penangkaran kepiting kelapa (Birgus latro) dilakukan pada bulan Juni - November 2004 dan Desember 2005 Februari 2006.  Pelaksanaan penelitian di lakukan di dua tempat, yaitu Laboratorium Sekolah Tinggi Kelautan dan Perikanan, Palu untuk percobaan kepiting dewasa, dan Laboratorium Ekobiologi Fakultas Perikanan dan Ilmu Kelautan IPB Bogor,  untuk percobaan kepiting tahap klomang.  Kepiting dewasa ditangkap dengan menggunakan beberapa peralatan a.l. perangkap, jaring dan secara langsung dengan tongkat.  Penangkaran dilakukan di bak-bak peliharaan berukuran 1x1x1 m3 yang dilengkapi dengan tempat persembunyian (30x15x15 cm3), dan bak kecil tempat air (7x10x10 cm3).  Sedangkan kepiting tahap klomang dipelihara di akuarium (80x40x40 cm3) yang diberi daun/serasah dan tempat air.  Hasil uji coba penangkaran kepiting kelapa dewasa menunjukkan bahwa tingkat survival rate berkisar 12,5-50%, dan tingkat mortalitasnya sebesar 50-87,5%.  Keadaan yang serupa juga dijumpai pada stadia klomang dimana survival ratenya sebesar 12,5% dan mortalitasnya sebesar 87,5%.  Dari uji coba makanan yang diberikan, diketahui bahwa kepiting dewasa kebanyakan lebih menyukai kelapa, dibandingkan jenis makanan yang berupa sayur ataupun ayam.
 Kata kunci:  penangkaran, kepiting kelapa (Birgus latro), makanan.
Relation between broodstock number and spawning frequency and egg production of humpback grouper (Cromileptes altivelis)
Authors: Syaifudin M. , Aliah R.S. , Muslim . , Sumantadinata K.
Published: February 2017

This study was performed to determine spawning frequency, number of ovulated egg and spawning time of humpback grouper (Cromileptes altivelis) broodstock in hatchery.  Broodstock of 20-83 fish in weight of 1.5-2.0 kg were reared in circular concrete tank 225 m3.  The results showed that increasing in number of broodstock increases spawning frequency (R2= 0.694), and ovulated eggs number was also increased (R2= 0.828).  Spawning of humpback grouper can occur in the third to fourth week in every month.

Keywords: reproductive biology, spawning, Cromileptes altivelis

 

ABSTRAK
 Penelitian ini bertujuan untuk mengetahui frekuensi pemijahan, jumlah telur dan waktu pemijahan populasi induk ikan kerapu tikus (Cromileptes altivelis) di hatchery.  Jumlah induk yang bervariasi antara 20-83 ekor dengan ukuran 1,5-2 kg ditempatkan dalam bak beton bulat, kapasitas 225 m3. Hasil penelitian ini menunjukkan bahwa dengan bertambahnya jumlah induk, maka frekuensi pemijahan yang diperoleh juga semakin meningkat (R2=0,694), dan produksi telur juga semakin meningkat (R2=0,828). Pemijahan kerapu tikus dapat berlangsung setiap bulan, di mana waktu pemijahan terjadi pada kuarter keempat hingga kuarter ketiga. Kata kunci: Biologi reproduksi, Pemijahan, Cromileptes altivelis
Use of Chitosan to Control Vibrio harveyi Infection on White Shrimp Litopeneaus vannamei
Authors: Sukenda . , Tri Anggoro Y. , Wahjuningrum D. , Rahman .
Published: February 2017

Immunostimulation and antibacterial effect of chitosan against vibriosis were examined in white shrimp (Litopenaeus vannamei).  Control shrimps were injected with 0.05 μl of sterile sea water, while experimental shrimps were injected with 2, 4 and 6 μg per g shrimp of chitosan.  All shrimps were subsequently challenged by 106 CFU/ml of live Vibrio harveyi by injection method.  Survival rate of shrimps injected with chitosan were found to slightly increase in accordance with dose of chitosan, even not statistically significant.  Total haemocyte count and phagocytic index at experimental shrimps were over than control shrimps up to three days post injection.  Number of V. harveyi in the intestine of experimental shrimps were lower than control shrimps indicates an antibacterial activity of chitosan to combat infection.

Keywords: chitosan, Vibrio harveyi, haemocyte, phagocytic index, Litopenaeus vannamei

 

ABSTRAK

Efek imunostimulasi dan antibakterial dari kitosan melawan vibriosis dilihat pada udang putih (Litopenaeus vannamei).   Udang control disuntik dengan 0,05 μl air laut steril, sedangkan udang uji disuntik dengan kitosan 2, 4 dan 6 μg per g udang.  Semua udang diuji tantang dengan 106 CFU/ml bakteri Vibrio harveyi hidup dengan metode penyuntikan.  Sintasan udang yang disuntik dengan kitosan meningkat berbarengan dengan peningkatan dosis kitosan, meskipun tidak menunjukkan perbedaan yang nyata.  Jumlah total hemosit dan indeks fagositosis pada udang lebih tinggi dibandingkan kontrol sampai tiga hari pasca penyuntikan.  Jumlah V. harveyi dalam saluran pencernaan dari udang uji lebih rendah dibandingkan udang kontrol, hal ini  menunjukkan aktifitas antibacterial dari kitosan dalam melawan infeksi.

Kata kunci: kitosan, Vibrio harveyi, hemosit, indeks fagositosis, Litopenaeus vannamei
Production of Paracheirodon innesi on Different Densities in Recirculating System
Authors: Budiardi T. , Gemawaty N. , Wahjuningrum D.
Published: February 2017

The objective of this research was to know production of neon tetra Paracheirodon innesi reared in recirculating system with density 20, 40 and 60 litre-1. Fish with 1.80 ± 0.04 cm lenghts were cultured in 30 x 20 x 20 cm aquarium in recirculating system, during 28 days. Result of this research showed that density of fish rearing affected  survival rate (p<0.05). No influence (p>0.05) was observed on body lenght, growth of body lenght and coefficient of varians.

Keywords: Density, production, growth, survival rate dan Paracheirodon innesi

 

ABSTRAK

Penelitian ini bertujuan untuk mengetahui produksi ikan neon tetra Paracheirodon innesi ukuran L yang dipelihara pada sistem resirkulasi dengan kepadatan 20, 40 dan 60 ekor/L. Benih ikan neon tetra yang digunakan berukuran sedang (medium) dengan panjang awal 1,80 ± 0,04 cm. Ikan ini dipelihara pada akuarium dengan ukuran 30cm x 20cm x 20cm selama 28 hari. Hasil penelitian ini menunjukkan bahwa kepadatan pemeliharaan memberikan pengaruh terhadap kelangsungan hidup (p<0,05).  Panjang tubuh, laju pertumbuhan panjang, pertambahan bobot harian dan koefisien keragaman panjang tidak terpengaruh oleh kepadatan pemeliharaan ikan (p>0,05).

Kata kunci : Padat penebaran, produksi, pertumbuhan, kelangsungan hidup, neon tetra dan
 Paracheirodon innesi
Study of the Influence Hormone LHRH-a and 17a-MT of Hemocytology on Orange Spot Grouper Broodstock, Epinephelus coioides
Authors: Johnny F. , Priyono A. , Roza D.
Published: February 2017

An experiment to study the influence of the chronic hormone LHRH-a and 17a-MT on haematology orange spot grouper, Epinephelus coioides broodstock was conducted at Gondol Research Institute for Mariculture, Bali.  Fish weights were ranged 4.7-10.4 kg (female) and 10.4-17.8 kg (male).  The females broodstock was treated by hormone 17a-MT with dosage 50 mg/kg body weight, and males was by hormone LHRH-a with dosage 50 and 100 mg/kg body weight.  Fish were kept on two concrete tank contained 100 m3 sea water and 2 meters depth and density 15 fishes/tank,  male ratio and female was 1 : 2.  Hormone treatment was peformed every two months until six months culture.  Blood were collected before treatment by hormone and every two months.  Hemocytology parameters measured were hematocrit, hemoglobin, total erythrocyte, and total leucocyte.  Result of the average indicator of hemacytology to influence on haemological parameters.

Keywords:  Epinephelus coioides, chronic hormone, hemocytology, orange spot grouper broodstock.

 
 ABSTRAK
Suatu percobaan dengan tujuan untuk mengetahui pengaruh  hormon LHRH-a dan 17a-MT terhadap keragaan hemositologi induk ikan kerapu lumpur, Epinephelus coioides telah dilakukan di Balai Besar Riset Perikanan Budidaya Laut Gondol, Bali.  Ikan uji yang digunakan adalah induk ikan kerapu lumpur kelamin betina dengan bobot antara 4,7-10,4 kg dan induk kelamin jantan dengan bobot antara 10,4-17,8 kg.  Ikan uji dipelihara dalam bak beton volume 100 m3 dengan kepadatan 15 ekor/bak, dilengkapi aerasi dan pergantian air sistim mengalir.  Induk kelamin betina diberikan perlakuan implantasi pelet hormon LHRH-a dosis 50 mg/kg bobot, dan induk kelamin jantan dengan hormon 17a-MT dosis 50 dan 100 mg/kg bobot ikan.  Implantasi dilakukan setiap 2 bulan, pada awal percobaan (sebelum perlakuan hormon/bulan ke-0), bulan ke-2, bulan ke-4, dan bulan ke-6 dilakukan sampling darah untuk pengujian keragaan hemositologi, meliputi; persentase hematokrit, kadar nilai hemoglobin, jumlah total eritrosit, dan jumlah total leukosit.   Hasil percobaan menunjukkan bahwa penggunaan hormon LHRH-a pada induk ikan kerapu lumpur kelamin betina dan hormon 17a-MT pada induk ikan kerapu lumpur kelamin jantan berpengaruh terhadap keragaan hemositologi.

Kata kunci:  Epinephelus coioides,  hemositologi, hormon kronik, induk ikan kerapu lumpur
Finansial Analysis of Wanayasa Tilapia Culture in Mekarsari Farmer Group
Authors: Diatin I. , Sobari Moch. P. , Irianni I.
Published: February 2017

This research aims to discover the general overview of the Nila Wanayasa's seeding business which conduct by Mekarsari's Conductors Group in Tanjungsari Village, to analyze the business' rent, to analyze the investment eligibility and to analyze the sensitivity of the price fluctuation of production factors, in this case is feed.  The business eligibility and its sensitivity judged by investment criteria i.e. NPV, Net B/C, and IRR.  The result shows the NPV is IDR 225,116,401.83, Net B/C is 19.38, and IRR is 707%.  The sensitivity analysis which using the switching value methods shows that the business is eligible to be continued with increasing price of feed until 800.917%, because of the NPV is zero, Net B/C is 1, and IRR is equal to the rate. 

Keywords:  financial analysis, NPV, Net B/C, IRR, Nila Wanayasa's Culture

 

ABSTRAK

Penelitian ini bertujuan untuk mengetahui kelayakan ekonomi usaha budidaya ikan nila Wanayasa yang dilakukan oleh kelompok tani Mekarsari, Desa Tanjungsari, Purwakarta.  Kriteria kelayakan usaha dan faktor sensitivitas yang diamati meliputi NPV, B/C net, dan IRR.  Hasil penelitian menunjukkan bahwa nilai NPV adalah sebesar Rp 225.116.401,83, B/C net sebesar 19,38, dan IRR 707%.  Analisa sensitivitas menggunakan metode "switching value" menunjukkan bahwa usaha petani layak dilanjutkan sampai harga pakan meningkat  800,92%, karena nilai NPV adalah nol, B/C net 1, dan IRR sama dengan tingkat suku bunga yang berlaku.

Kata kunci:  analisis finansial, NPV, B/C, IRR, Nila Wanayasa
Vitamin C Content in Daphnia sp. Enriched with L-ascorbic Acid Ethyl Cellulose at Different Enrichment Period
Authors: Jusadi D. , Meylani I. , Utomo N.B.P.
Published: February 2017

Daphnia with a density of 10,000 ind./l medium were enriched with either 0.1, 0.5 or 1 g l-ascorbic acid ethyl cellulose (VC) per l culture medium. In this triplicate experiment, Daphnia were enriched with VC in a 12 litre aquaria for 0, 3, 6 or 9 h. Results showed that the maximum VC content in Daphnia enriched with 0.1, 0.5 and 1 g VC/l medium was 347, 471 and 482 mg VC/g Daphnia (dry basis), respectively. This VC content was obtained after 5 h, 5 h 12 min and 5 h 18 min of enrichment period.  During 9 h enrichment period, the survival rate of Daphnia enriched with 0.1, 0.5 and 1 g VC/l medium was decrease linearly from 100% to 55.6%, 50.1% and 47.9%, respectively.

Keywords: Daphnia, vitamin C, enrichment

 

ABSTRAK

Dalam penelitian ini,  Daphnia dengan padat tebar 10.000 ekor/l diperkaya dengan vitamin C (VC) pada tiga dosis yang berbeda, yaitu dosis 0,1, 0,5 dan 1 g VC/l media untuk masing-masing penelitian 1, 2 dan 3.  Daphnia diperkaya dalam wadah volume 12 liter selama 0, 3, 6 dan 9 jam.  Masing-masing perlakuan diulang sebanyak 3 kali ulangan. Hasil analisa respon menunjukkan bahwa waktu pengkayaan yang optimum untuk masing-masing dosis pengkayaan 0,1, 0,5 dan 1 g VC/l media secara berturut-turut adalah 5 jam, 5 jam 12 menit dan 5 jam 18 menit dengan kadar VC masing-masing sebesar 347, 471 dan 482 mg VC/g Daphnia.  Tingkat kelangsungan hidup Daphnia sp. menunjukkan terjadinya penurunan secara linier dengan semakin bertambahnya waktu pengkayaan.  Tingkat kelangsungan hidup Daphnia sp. setelah 9 jam masa pengkayaan ialah sebesar 55,63%, 50,12% dan 47,92% untuk dosis 0,1, 0,5 dan 1 g VC/l media. 

Kata kunci: Daphnia, vitamin C, pengkayaan