8-isoprostane as a marker of oxidative stress in experimental animals after implantation of surgical mesh with a coating based on tantalum and its derivatives
Authors:   Kharkiv National Medical University, NAKONECHNA O. A. , KYSLOV O. V. ,   Kharkiv National Medical University
Published: February 2024

Nowadays, the world observes the problem of adhesion process after implantation of polypropylene surgical meshes, which occurs in about 30–40 % of patients in the postoperative period. Over the past 30 years, tantalum and its derivatives have proven themselves as materials for biomedical implants in dentistry and orthopedics. In the future, tantalum-based coatings may become an inhibitor of the development of adhesion and inflammatory processes after surgical mesh implantation. In order to better predict the implantation of biomedical materials, it is important to determine the markers of the development of oxidative stress. A change in the concentration of biomarkers of the state of the oxidants system, in particular 8-isoprostane, can indicate the development of pathological conditions under the conditions of oxidative stress, which can have a significant impact on the viability of cells after surgical intervention. The aim of the study is to determine the concentration of 8-isoprostane in the blood of rats after implantation of surgical meshes coated with tantalum and tantalum oxide. Materials and methods. The experimental group included 36 male rats of the WAG population weighing 240 ± 20 g. A 15x15 mm polypropylene surgical mesh was surgically implanted between the abdominal wall and various sections of the colon. Anesthesia was performed using the intraperitoneal route of administration of the drug «Relax» BioTestLab, Ukraine) at a dose of 8 mg/kg. After 28 days, the experimental animals were decapitated by cervical dislocation, and blood was immediately collected in sterile K2 EDTA VACUTAINER tubes (BD Vacutainer®). Blood samples were used to determine the content of 8-isoprostane in the blood by enzyme-linked immunosorbent assay using the «8-isoprostane EIA KIT» kit (Cayman Chemical Company, USA) according to the manufacturer’s instructions on the «Solar» PV-1251C spectrophotometer. Results and discussion. The content of 8-isoprostane in blood plasma was statistically 158,6 % higher in the experimental group implanted with uncoated surgical mesh and 171,5 % higher in the experimental group implanted with polypropylene surgical mesh with tantalum nitride coating in comparison with the results of the control group. The concentration of 8-isoprostane in the blood of rats after implantation of polypropylene surgical meshes with a coating based on tantalum and tantalum oxide was higher by 102,6 % and 111,1 %, respectively, compared to the results obtained in the group of intact animals, which indicates their better biocompatibility compared to the results of the groups implanted with uncoated and coated tantalum nitride surgical mesh. Conclusion. The obtained results indicate that coatings based on tantalum and tantalum oxide inhibit lipid peroxidation and demonstrate excellent biocompatibility and anti-inflammatory effect, which makes it possible to consider their future use in hernioplasty.