bluuebunny/crossref_metadata_2025 huggingface dataset:
P.077 Telemedicine in pediatric neurology: a survey of patient and provider experience
Authors: Strasser L , Hayawi L , Webster R , Venkateswaran S , Muir K
Published: June 2023

Background: Prior to the pandemic, telemedicine use was limited and sparsely funded within Ontario. During the pandemic, a shift in clinical recommendations and government funding models promoted telemedicine. We aim to highlight both quantitative and qualitative aspects of the patient and provider experience over 2.5 years within a Canadian Pediatric Neurology clinic. Main objectives of the study are to assess the safety, efficiency and convenience of telemedicine. Methods: A REDCap survey was sent to all patients with a telemedicine appointment from March 2020 –September 2022 and all Pediatric Neurology providers. Survey included a 5-point Likert scale questions, open questions, and patient characteristics. Results: Responses received from 272 patients and 7 providers. 91% of patients and all providers were satisfied with telemedicine. 95% of patients and all providers felt they received or were able to provide safe/adequate care. 90% of patients and all providers reported that telemedicine was more convenient. 87% of patients and all providers were interested in future appointments via telemedicine. Conclusions: Our survey shows patients and providers had highly positive experiences with telemedicine – reporting care was adequate, safe, and more convenient. This data supports incorporating telemedicine into future care and advocates that Canadian regulations/billing codes to continue to support telemedicine.
P.078 Desynchronization in high-definition transcranial direct current stimulation of refractory status epilepticus
Authors: Toutant D , El-Alawi H , Choi E , Wright N , Khanam M , Paunovic B , Ko J , Ng M
Published: June 2023

Background: Recently, we showed cathodal high-definition transcranial direct current stimulation (HD-tDCS) inhibits spikes in refractory status epilepticus (RSE) patients. We sought to characterize relative power changes within regional frequency bands to explore connectivity throughout stimulation. Methods: 28 ICU-EEGs from 10 patients were filtered with 1Hz-lower/127Hz-upper/60Hz-notch. Power spectral density was computed for each individual bipolar-longitudinal (excluding midline) electrode chain in delta/theta/alpha/beta/gamma frequency bands. Relative power was extracted throughout recordings by Welch’s method with 2.5-second Hanning window size with 50% overlap every 10 seconds. The average of relative powers from ≤20-minute pre/during/post stimulation windows were calculated per session/chain/band. Absolute maximal relative power change between pre-during and during-post were computed. ANOVA statistically tested for differences. Results: Change in pre-during for delta/theta/alpha/beta/gamma were respectively -0.7%/-3.1%/-2.8%/-2.1%/8.2%, and during-post were 1.3%/2.8%/2.3%/1.7%/-7%. Positive average changes are increases in average relative power where negative changes show decreases. Changes in absolute maximal average relative power between pre-during and during-post stimulation between frequency bands were significant respectively (p=0.0011, p=0.0139). Conclusions: Relative power in all bands drop pre-during and increase during-post except gamma which correlates with desynchronization being a mechanism for HD-tDCS efficacy in RSE. These frequency band power changes may imply brain connectivity changes that need to be further explored.
P.107 Endocrine and vision outcomes following 90Yttrium therapy for cystic sellar lesions: a prospective cohort study
Authors: MacLean MA , Ahmad S , Hebb AL , Tahir A , Greene R , Burrell S , Clarke DB , Imran SA
Published: June 2023

Background: Due to high risk of recurrence and complications associated with microsurgical resection or aspiration of enlarging cystic sellar lesions, stereotactic intracavitary irradiation with 90Yttrium (90Yt) has been proposed as an alternative to mitigate these challenges. Long-term efficacy data for this procedure is lacking. Methods: We conducted a Health Canada approved, single-centre, prospective cohort study to assess the effect of 90Yt therapy on cyst volume, visual field (VF) testing and endocrine function. All patients underwent right frontal stereotactic insertion of 90Yt colloid (200 Gy cyst wall). Results: 22 patients [mean age = 63.5 (± 15.4) years] received 90Yt therapy. Mean follow-up was 52 (5-113) months. Cysts included: craniopharyngioma (86%), Rathke’s cleft cyst (9%) and cystic prolactinoma (5%). Mean cyst volume reduction was 77% at 12-months (5.2 ± 4.5 cc to 1.2 ± 1.5 cc; p = 0.002). Rate of pre- and post-op hormonal dysfunction was 59% and 68%, respectively (p = 0.50). Pre-operative VF deficits were found in 68% of patients, of which 32% normalized and 36% remained stable (p = 0.180). Worsened vision was detected in 9% of patients post-operatively. Conclusions: 90Yttrium therapy significantly reduces sellar cyst size without having a deleterious effect on vision and endocrine function.
P.109 Meningioma with intraparenchymal abscess: a case report and review of the literaure
Authors: Kulamurugan A , Yasuda E , Alhantoobi M , Reddy K
Published: June 2023

Background: Intracranial intratumoural abscesses are rare occurrences typically treated with antibiotics and possible surgical resection. This study describes a meningioma-associated abscess and a review of the literature. Methods: Medical records and investigations were reviewed. A literature search of PubMed was completed. Results: A 56-year-old male presented with septic shock and dysuria. Urine culture isolated E. Coli, and he was treated with Ertapenem prior to discharge. A CT scan was ordered during hospitalization for unrelenting headaches, revealing a meningioma. Conservative management with follow-up as an outpatient was decided. However, he returned within two weeks with a fever and progressive left-sided weakness. A right frontal craniotomy for tumour resection was performed, and culture of necrotic-appearing tissue within the tumour revealed E. Coli. He was treated with Meropenem for six weeks, and at follow-up, the patient was asymptomatic. Our scoping review illustrated that 18 meningioma-associated abscesses have been reported in the literature since the first report in 1994. Conclusions: This case highlights the hematogenous spread of a urinary infection, resulting in an intratumoural abscess. Review of the literature indicated that, similarly, 39% of cases had recent or concurrant urinary tract infections. Future studies should seek to determine conclusive guidelines for diagnosing intratumoural abscesses.
P.113 Impact of 5-ALA on rates of complete high grade glioma resection: a Canadian perspective
Authors: Catana D , Malone J , Sinclair J
Published: June 2023

Background: The relationship between glioblastoma extent of surgical resection (EoR) and survival is well documented.1-3 The advent of 5-aminolevulinic acid (5-ALA), a tissue selective fluorophore, has led to increased rates of gross total tumour resection.4-6 Since 5-ALA received approval for use in Canada in 2020, no Canadian centres have examined its impact on rates of complete resection (CR) for newly diagnosed high grade glioma (HGG) patients. Methods: This study evaluates the difference in EoR class7, for newly diagnosed HGG. Fifty-one consecutive patients underwent awake craniotomy with white light illumination (WLS) while 45 consecutive HGG patients were operated with fluorescence guidance (FGS). Analysis of EoR class was blinded and performed by 2 independent reviewers with a third adjudicator available for discrepancies. Residual tumour volumes were quantified by segmentation of postoperative 1mm slice MRI. Results: The FGS group was found to have: 80% complete resection (CR), 11% near-total resection (NTR), and 9% subtotal resection (STR). This compared favourably to the WLS respective rates of 67%, 6%, and 28%. Conclusions: For awake craniotomy protocol, the odds of complete resection were higher in the FGS group, compared to the WLS group (OR = 2; 95% CI 1.06, 2.93).
P.117 Quantitative evaluation in competency-based medical education – a nationwide survey of the spinal surgical training landscape
Authors: Hatoum R , Muddaluru V , Martyniuk A , Yarascavitch B , Pahuta M , Guha D
Published: June 2023

Background: The competency-based medical education (CBME) model has been recently implemented in spinal neurosurgical and orthopedic residencies. This model is grounded on entrustable professional activities (EPAs) that allow the assessment of clinical milestones. Integrating quantitative metrics to evaluate procedural competencies could refine the assessment’s scope. This survey, administered to program directors (PDs), aims to evaluate the current state and anticipated needs for quantitative evaluation to develop innovative assessment techniques. Methods: We surveyed 32 PDs of neurosurgical (N=14) and orthopedics (N=16) programs with a spine service via RedCap. We collected information on the programs’ characteristics. We inquired about existing assessment methods and the perceived values of developing quantitative metrics to assess spinal technical competencies using thirteen questions. Results: The response rate was 53%. All programs use EPAs to assess procedural competencies through direct observation in the operation room. One surgical program employed quantitative metrics for examination. Four PD valued the profitability of quantitative evaluation methods in a clinical or simulatory context. Conclusions: The use of quantitative metrics to assess spinal surgical competencies in Canadian neurosurgical and orthopedic residency programs is seldom. Despite its underutilization, PDs acknowledge the potential for quantitation to improve the accuracy and reliability of CBME assessments in both simulated and clinical settings.
P.140 Illustrated case report: CSF shunt peritoneal catheter obstruction due to omental adhesion and fat stranding
Authors: Albeshri AS , Khayat H , Kessrwan M , Yasuda E , Alsunbul WA , Reddy K , Moore J
Published: June 2023

Background: CSF shunt obstruction secondary to omental adhesion alone, without cyst or pseudocyst formation, is rarely reported in the literature. Here we present a case of distal catheter obstruction due to omental wrapping with an atypical presentation of shunt failure. CT imaging demonstrated omental stranding. The shut revision was entirely laparoscopic. The case is supplemented with intraoperative images. Methods: Chart review and literature search. Results: 33-year-old female with right-sided cystoperitoneal shunt for posterior fossa arachinoid cyst who presented to ED with a picture of a surgical abdomen suggestive of acute cholecystitis. Interestingly, this patient had a gallbladder removed a year ago. CT abdomen showed non-specific findings of omental fat stranding around the tip of the catheter. Although this patient had no headache or any neurological symptoms, CT brain was done and showed increase in the cyst size. Diagnostic laparoscopy showed Intraoperative findings suggestive of active omental role in the aetiology of this shunt malfunction. After adequate adhesiolysis, the catheter was noted to be working and dripping CSF and repositioned into the peritoneal cavity. Conclusions: Shunt malfunctions due to omentoperitoneal adhesion is rarely reported but may in fact be under-recognized. This requires high index of suspicion especially in case of equivocal imaging.
P.149 Enhanced Recovery after Surgery (ERAS) in Anterior Cervical Discectomy and Fusion (ACDF). A single center retrospective analysis of prospective collected data
Authors: Ndongo Sonfack D , Wang Z , Boubez G , Shen J , Shedid D , Yuh S
Published: June 2023

Background: Enhanced recovery after surgery (ERAS) concept has been implemented in many surgical specialties, with improved surgical and clinical outcomes, all while decreasing hospital cost. We have established a single center ERAS protocol for Anterior Cervical Discectomy and Fusion (ACDF) same-day surgeries. Methods: A retrospective analysis of prospective collected data of all patients undergoing one to two level ACDF surgeries at the CHUM based on a patient selection criteria’s list. Demographics, BMI, patient report outcome measures (NDI, VAS, mJOA), operative time, wake up time, time in recovery room and time till discharge were analysed. Complications were also noted. Results: When compared to our pre-ERAS group, our study showed that an ERAS protocol in select patients is not associated with any peri-operative complications, no 30-day / 90-day readmissions, nor any conversion to admission. All patients were safely discharged as planned. Operative length was similar. There was no dysphagia noted. There was improvement in all PROMs. Conclusions: ERAS protocol for ACDF same day surgeries in carefully selected patients is safe and provide same clinical outcomes. Meticulous surgical techniques, close post operative observation with a follow-up telephone call the next day are all key in assuring a safe and successful surgery and patient discharge.
C.1 The molecular diagnostic landscape of children with seizure onset in the first three years of life
Authors: McKnight D , McLaughlin H , Grayson C , Sherrington R , Butterfield N , Aradhya S , DeRienzo L , Morales A , Willcock A , Johnson B
Published: June 2023

Background: To clarify the landscape of molecular diagnoses (MDs) in early-onset epilepsy individuals, we determined the prevalent MDs stratified by age at seizure onset (SO) and the time to MD in children with SO <36 months of life. Methods: A panel of up to 302 genes associated with epilepsy was utilized and ordering physicians provided the age of SO. Diagnostic yield analyses were performed for SO ages including <1 mo, 1-2 mo, 3-5 mo, 6-11 mo, 12-23 mo, and 24-35 mo. The time to MD (MD age - SO age) was determined for the top 10 genes in each SO category. Results: 15,074 individuals with SO <36 months of life were tested. Predominant MD findings are as follows: KCNQ2 in neonates with SO at <1mo, KCNQ2 and CDKL5 for SO between 1-2 mo, PRRT2 and SCN1A for SO between 3-11 mo, and SCN1A for SO between 12-36 months. The median time to MD varied by gene. For example, there was no delay in the median time to MD for the GLDC, KCNQ2, and SCN2A genes while the median delay for MECP2, SLC2A1, and other genes was ≥ 12 months. Conclusions: These data highlight the importance of comprehensive early testing in children with early-onset epilepsy.
Geometrical sets with forbidden configurations
Authors: Castro-Silva Davi
Published: June 2023

Given finite configurations

$P_1, \dots , P_n \subset \mathbb {R}^d$

, let us denote by

$\mathbf {m}_{\mathbb {R}^d}(P_1, \dots , P_n)$

the maximum density a set

$A \subseteq \mathbb {R}^d$

can have without containing congruent copies of any

$P_i$

. We will initiate the study of this geometrical parameter, called the independence density of the considered configurations, and give several results we believe are interesting. For instance we show that, under suitable size and nondegeneracy conditions,

$\mathbf {m}_{\mathbb {R}^d}(t_1 P_1, t_2 P_2, \dots , t_n P_n)$

progressively ‘untangles’ and tends to

$\prod _{i=1}^n \mathbf {m}_{\mathbb {R}^d}(P_i)$

as the ratios

$t_{i+1}/t_i$

between consecutive dilation parameters grow large; this shows an exponential decay on the density when forbidding multiple dilates of a given configuration, and gives a common generalization of theorems by Bourgain and by Bukh in geometric Ramsey theory. We also consider the analogous parameter

$\mathbf {m}_{\mathbb {S}^d}(P_1, \dots , P_n)$

in the more complicated framework of sets on the unit sphere

$\mathbb {S}^d$

, obtaining the corresponding results in this setting.
“My Heart Is a Piece of Stone”: Anxious Separations and Emotional Dislocations in British Correspondence from the Long Second World War
Authors: Summerfield Penny
Published: June 2023

Historians who write about emotion in wartime focus mainly on the experiences of front-line soldiers and of civilians under bombardment exposed to life-threatening events. However, in Britain in World War II, conscription, mobilization, and evacuation inflicted hugely disruptive separations on a large proportion of the population, and the emotions that they provoked have been under-examined. This paper excavates emotion in an unusually complete set of letters written by a British working-class couple between 1941 and 1946. Interpreting letter writing as a technology of the self, it explores their letter-writing practices and uses psychoanalytic theory to comprehend the anxieties that their letters document. Wartime and postwar separation, enforced by conscription, challenged their aspirations to a companionate marital style and added to the complexities of pregnancy and parenthood. The sickness and hospitalization of their baby in 1945–46, in the era before the establishment of the National Health Service, introduced a new dimension to separation. Occurring at a time when the couple were even further apart geographically than during the war itself and letters were the only regular means of connection, this trauma imposed massive marital and, particularly, maternal strain. By analyzing and contextualizing the increasingly fraught exchanges between a mother on her own and a man at the front line, this article throws new light on epistolary constructions of anxious separations and emotional dislocations in the long Second World War.
Provocation by Witchcraft: Exploring the Evolution of the Kenyan Courts’ Interpretation of the Doctrine of Provocation in Relation to Witchcraft Beliefs
Authors: Owusu Emmanuel Sarpong
Published: June 2023

The belief in witchcraft and sorcery is a significant cause of intentional homicide in Kenya. Moreover, those who kill people suspected of being witches often employ as a defense for their actions the so-called provocation by witchcraft argument: the homicide was purportedly committed under the influence of belief in witchcraft and sorcery. One major legal difficulty that the Kenyan courts have frequently been invited to resolve is thus the question as to whether the belief in witchcraft and sorcery avails to an accused person the defense of grave provocation and, if so, under what circumstances. Drawing largely on pertinent case law, statutes, and academic literature, the author explores the controversy over provocation by witchcraft. The author first offers an exposition of the concept of witchcraft and sorcery in Africa and critically discusses the evolution of the Kenyan courts’ interpretation of the country’s law on provocation in relation to witchcraft beliefs since the 1930s. The author establishes that under the current Kenyan common law, defenses of heat of passion and sudden provocation may apply in instances where there is no real provocation and that the courts have exceeded the boundaries of the provocation defense without well-grounded reasons. The author cautions that giving the doctrine of provocation such a broad construction and application may increase the already rampant killings of suspected witches in Kenya.
Exact convergence analysis for metropolis–hastings independence samplers in Wasserstein distances
Authors: Brown Austin , Jones Galin L.
Published: June 2023

Under mild assumptions, we show that the exact convergence rate in total variation is also exact in weaker Wasserstein distances for the Metropolis–Hastings independence sampler. We develop a new upper and lower bound on the worst-case Wasserstein distance when initialized from points. For an arbitrary point initialization, we show that the convergence rate is the same and matches the convergence rate in total variation. We derive exact convergence expressions for more general Wasserstein distances when initialization is at a specific point. Using optimization, we construct a novel centered independent proposal to develop exact convergence rates in Bayesian quantile regression and many generalized linear model settings. We show that the exact convergence rate can be upper bounded in Bayesian binary response regression (e.g. logistic and probit) when the sample size and dimension grow together.
CONCAVITY PROPERTY OF MINIMAL INTEGRALS WITH LEBESGUE MEASURABLE GAIN
Authors: GUAN QI’AN , YUAN ZHENG
Published: June 2023

In this article, we present a concavity property of the minimal
$L^{2}$
integrals related to multiplier ideal sheaves with Lebesgue measurable gain. As applications, we give necessary conditions for our concavity degenerating to linearity, characterizations for 1-dimensional case, and a characterization for the holding of the equality in optimal
$L^2$
extension problem on open Riemann surfaces with weights may not be subharmonic.
Climate change intensified violence in the south-central Andean highlands from 1.5 to 0.5 ka
Authors: Snyder Thomas J. , Haas Randall
Published: June 2023

The archaeology of the pre-contact Andes provides an ideal study of human responses to climate change given the region's extreme climatic variability, excellent archaeological preservation, and robust paleoclimate records. We evaluate the effects of climate change on the frequency of interpersonal violence in the south-central Andes from ca. 1.5–0.5 ka (AD 470–1540) by comparing incidents of skeletal trauma observed among 2753 crania from 58 sites to rates of ice accumulation at the Quelccaya Glacier. We find that, in the highlands, the odds of identifying inter-personal violence increase on average by a multiplicative factor of 2.4 (1.8–3.2; 95% C.I.) for every 10-centimeter decrease in annual ice accumulation. Our statistical analysis does not detect a relationship between ice accumulation and interpersonal violence rates among coastal or mid-elevation populations. This disparity likely resulted from variable economic and sociopolitical strategies at different elevations. The failure of rain-fed agriculture during periods of drought and concomitant dissolution of organizing polities likely predisposed highland populations to socioeconomic stress and violent competition for limited resources. Conversely, diversity among lowland and midland economies may have buffered against the effect of drought.
Playing the Police with the Agitprop Troupes of Weimar Germany
Authors: Piggott Jessi
Published: June 2023

Germany's amateur agitprop theatre movement produced some of the most popular, pervasive, and politically contentious art in the Weimar Republic, not least because of the way performers inserted themselves into the fabric of working-class life with the unequivocal intention of politicizing audiences. Germany's first agitprop troupes formed within youth clubs affiliated with the Communist Party (KPD) around 1925, but the movement quickly grew beyond established club culture, with troupes sprouting up “like mushrooms,” as one critic of the period put it. By 1929 police estimated there were about two hundred self-proclaimed agitprop troupes spread across Germany, all pursuing a transparently aggressive political agenda: to turn the theatre into a site of revolutionary class struggle. If the Weimar period saw an unprecedented mixing of art and politics, agitprop took this tendency to the extreme by declaring theatre to be a weapon in the hands of the proletariat. As the slogan of the 1931 International Meeting of Agitprop Troupes in Cologne put it: “Workers’ theatre is class struggle.”
Parental Self-Efficacy Among Israeli Older Adults
Authors: Kestler–Peleg Miri , Greenblatt-Kimron Lee , Even-Zohar Ahuva , Lavenda Osnat
Published: June 2023

Parental self-efficacy (PSE) is a central aspect of parenting research. Although parenting continues in older ages, few studies have focused on PSE among older adults. In line with the parenting determinant model, the study examined the associations between psychological distress, social support, loneliness, and PSE in older age.

The study used a quantitative cross-sectional survey, including 362 Israeli parents age 65 years and above. Participants were asked to complete questionnaires of psychological distress, social support, loneliness, PSE, and background information.

Results revealed that higher PSE was associated with lower psychological distress, higher social support, and a lower sense of loneliness in older adults. The research model explained 36.9 per cent of the variance in PSE among older adults.

Mental state and social conditions are important for PSE of older adults; therefore, professionals should ensure the availability of diverse solutions to enable, maintain, and promote well-being through social and familial engagement in this population.
Mental health among bereaved youth in the ALSPAC birth cohort: Consideration of early sociodemographic precursors, cognitive ability, and type of loss
Authors: Denckla Christy A. , Espinosa Dice Ana Lucia , Slopen Natalie , Koenen Karestan C. , Tiemeier Henning
Published: June 2023

Background:

Bereaved youth are at greater risk for adverse mental health outcomes, yet less is known about how social context shapes health for bereaved children. Ecosocial theory is employed to conceptualize bereavement in the context of sociodemographic factors.

Method:

This longitudinal study used data from the Avon Longitudinal Study of Parents and Children. Of the 15,454 pregnancies enrolled, 5050 youth were still enrolled at age 16.5 and completed self-report questionnaires on life events and emotional/behavioral symptoms.

Results:

Sociodemographic precursors associated with parent, sibling, or close friend bereavement included maternal smoking, parental education levels, and financial difficulties. The significant yet small main effect of higher cognitive ability, assessed at age 8, on reduced emotional/behavioral symptoms at age 16.5 (β = −0.01, SE = 0.00, p < 0.001) did not interact with bereavement. Bereavement of a parent, sibling, or close friend was associated with a 0.19 point higher emotional/behavioral symptom log score compared to non-bereaved youth (95% CI: 0.10–0.28), across emotional, conduct, and hyperactivity subscales.

Conclusions:

Descriptive findings suggest sociodemographic precursors are associated with bereavement. While there was an association between the bereavement of a parent, sibling, or close friend and elevated emotional/behavioral symptoms, cognitive ability did not moderate that effect.
Deregulation as a source of China’s economic growth
Authors: Pan Shiyuan , Xu Kai , Zhao Kai , Chen Tianxu
Published: June 2023

We develop a simple two-sector neoclassical growth model in which the upstream sector produces intermediate goods, and the downstream sector produces final goods with outputs from the upstream. While the downstream sector features perfect competition, firms in the upstream sector engage in Cournot competition and charge a markup. We show that the deregulation and the introduction of competition in the upstream goods sector not only increases the productivity in the sector but also has a substantial spillover effect on the productivity of the downstream sector and factor prices. We calibrate the model to the Chinese economy and use the calibrated model to quantitatively evaluate the extent to which the deregulation in the upstream market in China from 1998 to 2006 can account for the rapid economic growth and the high and rising returns to capital in China over the same period. Our quantitative experiments show that the deregulation in the upstream sector can account for a significant share of economic growth in China during the study period. In addition, our model delivers implications that are consistent with several other relevant observations in China during the same period.
Cooking behaviours after Diabetes Prevention Program (DPP) participation among DPP participants in Baltimore, MD
Authors: Russell Lauren E , Tse Jillian , Bowie Janice , Richardson Caroline R , Trubek Amy , Maruthur Nisa , Wolfson Julia A
Published: June 2023

Objective:

The Diabetes Prevention Program (DPP) is a widely implemented 12-month behavioural weight loss programme for individuals with prediabetes. The DPP covers nutrition but does not explicitly incorporate cooking skills education. The objective of the current study is to describe food and cooking skills (FACS) and strategies of recent DPP participants.

Design:

Photo-elicitation in-depth interviews were conducted from June to August, 2021.

Setting:

Baltimore, MD, USA.

Participants:

Thirteen Black women who participated in DPP.

Results:

The DPP curriculum influenced participants’ healthy cooking practices. Many participants reported shifting from frying foods to air-frying and baking foods to promote healthier cooking and more efficient meal preparation. Participants also reported that their participation in DPP made them more mindful of consuming fruits and vegetables and avoiding foods high in carbohydrates, fats, sugars and Na. With respect to food skills, participants reported that they were more attentive to reading labels and packaging on foods and assessing the quality of ingredients when grocery shopping.

Conclusions:

Overall, participants reported changing their food preferences, shopping practices and cooking strategies to promote healthier eating after completing the DPP. Incorporating hands-on cooking skills and practices into the DPP curriculum may support sustained behaviour change to manage prediabetes and prevent development of type 2 diabetes among participants.
Pollinators and policy: the intersecting path of various actors across an evolving CAP
Authors: Giovanetti Manuela , Bortolotti Laura
Published: June 2023

Pollinators are a crucial group of organisms due to their role in the maintenance of environmental biodiversity and crop production. Pollinators may be very diverse; however, in temperate areas they are mainly insects, and the most active and important are certainly the bees. Not only the well-known honeybee: there are almost 2000 species of wild bees in Europe, and about 1000 just in Italy. There is an increasing public worry related to their decline and to the impact that this decline may have on agricultural production. The way public perception of the pollinator issue arises is generally neglected, especially by important stakeholders such as the farmers. Moreover, direct actions were not indicated by the policy till recently. We surveyed the main steps that possibly drove the current political perspective at the European and national (Italian) level. We found an increased boost toward healthier environments through the release of various documents. To look for changes that may include pollinator protection, we need to address the Common Agricultural Policies (CAPs), the tool that shapes the European agro-environments. A new CAP document has been recently released, and the Member States are moving toward the definition of eco-schemes to be adopted by farmers after payments. Italy placed pollinators in pole position with a dedicated eco-scheme: will pollinators finally be acknowledged?
2-Oxabicyclo[2.1.1]hexanes as saturated bioisosteres of the ortho-substituted phenyl ring
Authors: Denisenko Aleksandr , Garbuz Pavel , Voloshchuk Nataliya M. , Holota Yuliia , Al-Maali Galeb , Borysko Petro , Mykhailiuk Pavel K.
Published: June 2023

The ortho-substituted phenyl ring is a basic structural element in chemistry. It is found in more than three hundred drugs and agrochemicals. During the past decade, scientists have tried to replace the phenyl ring in bioactive compounds with saturated bioisosteres to obtain novel patentable structures. However, most of the research in this area has been devoted to the replacement of the para-substituted phenyl ring. Here we have developed saturated bioisosteres of the ortho-substituted phenyl ring with improved physicochemical properties: 2-oxabicyclo[2.1.1]hexanes. Crystallographic analysis revealed that these structures and the ortho-substituted phenyl ring indeed have similar geometric properties. Replacement of the phenyl ring in marketed agrochemicals fluxapyroxad (BASF) and boscalid (BASF) with 2-oxabicyclo[2.1.1]hexanes dramatically improved their water solubility, reduced lipophilicity and most importantly retained bioactivity. This work suggests an opportunity for chemists to replace the ortho-substituted phenyl ring in bioactive compounds with saturated bioisosteres in medicinal chemistry and agrochemistry.
A multi-enzyme machine polymerizes the Haemophilus influenzae type b capsule
Authors: Cifuente Javier O. , Schulze Julia , Bethe Andrea , Di Domenico Valerio , Litschko Christa , Budde Insa , Eidenberger Lukas , Thiesler Hauke , Ramón Roth Isabel , Berger Monika , Claus Heike , D’Angelo Cecilia , Marina Alberto , Gerardy-Schahn Rita , Schubert Mario , Guerin Marcelo E. , Fiebig Timm
Published: June 2023

Bacterial capsules have critical roles in host-pathogen interactions. They provide a protective envelope against host recognition, leading to immune evasion and bacterial survival. Here we define the capsule biosynthesis pathway of Haemophilus influenzae serotype b (Hib), a Gram-negative bacterium that causes severe infections in infants and children. Reconstitution of this pathway enabled the fermentation-free production of Hib vaccine antigens starting from widely available precursors and detailed characterization of the enzymatic machinery. The X-ray crystal structure of the capsule polymerase Bcs3 reveals a multi-enzyme machine adopting a basket-like shape that creates a protected environment for the synthesis of the complex Hib polymer. This architecture is commonly exploited for surface glycan synthesis by both Gram-negative and Gram-positive pathogens. Supported by biochemical studies and comprehensive 2D nuclear magnetic resonance, our data explain how the ribofuranosyltransferase CriT, the phosphatase CrpP, the ribitol-phosphate transferase CroT and a polymer-binding domain function as a unique multi-enzyme assembly.
How to establish and maintain a multimodal animal research dataset using DataLad
Authors: Kalantari Aref , Szczepanik Michał , Heunis Stephan , Mönch Christian , Hanke Michael , Wachtler Thomas , Aswendt Markus
Published: June 2023

Sharing of data, processing tools, and workflows require open data hosting services and management tools. Despite FAIR guidelines and the increasing demand from funding agencies and publishers, only a few animal studies share all experimental data and processing tools. We present a step-by-step protocol to perform version control and remote collaboration for large multimodal datasets. A data management plan was introduced to ensure data security in addition to a homogeneous file and folder structure. Changes to the data were automatically tracked using DataLad and all data was shared on the research data platform GIN. This simple and cost-effective workflow facilitates the adoption of FAIR data logistics and processing workflows by making the raw and processed data available and providing the technical infrastructure to independently reproduce the data processing steps. It enables the community to collect heterogeneously acquired and stored datasets not limited to a specific category of data and serves as a technical infrastructure blueprint with rich potential to improve data handling at other sites and extend to other research areas.
A spike in circulating cytokines TNF-α and TGF-β alters barrier function between vascular and musculoskeletal tissues
Authors: Ngo Lucy , Knothe Tate Melissa L.
Published: June 2023

Molecular transport between the circulatory and musculoskeletal systems regulates articular joint physiology in health and disease. Osteoarthritis (OA) is a degenerative joint disease linked to systemic and local inflammation. Inflammatory events involve cytokines, which are secreted by cells of the immune system and modulate molecular transport across tissue interfaces (referred to as tight junction [TJ] barrier function). In a previous study from our group, OA knee joint tissues were shown to exhibit size separation of different sized molecules delivered as a single bolus to the heart (Ngo et al. in Sci. Rep. 8:10254, 2018). Here, in a follow up study of parallel design, we test the hypothesis that two common cytokines, with multifaceted roles in the etiology of osteoarthritis as well as immune state in general, modulate the barrier function properties of joint tissue interfaces. Specifically, we probe the effect of an acute cytokine increase (spike) on molecular transport within tissues and across tissue interfaces of the circulatory and musculoskeletal systems. A single bolus of fluorescent-tagged 70 kDa dextran, was delivered intracardially, either alone, or with either the pro-inflammatory cytokine TNF-α or the anti-inflammatory cytokine TGF-β, to skeletally mature (11 to 13-month-old) guinea pigs (Dunkin-Hartley, a spontaneous OA animal model). After five minutes' circulation, whole knee joints were serial sectioned and fluorescent block face cryo-imaged at near-single-cell resolution. The 70 kDa fluorescent-tagged tracer is analogous in size to albumin, the most prevalent blood transporter protein, and quantification of tracer fluorescence intensity gave a measure of tracer concentration. Within five minutes, a spike (acute doubling) in circulating cytokines TNF-α or TGF-β significantly disrupted barrier function between the circulatory and musculoskeletal systems, with barrier function essentially abrogated in the TNF-α group. In the entire volume of the joint (including all tissue compartments and the bounding musculature), tracer concentration was significantly decreased in the TGF-β- and TNF-α- compared to the control-group. These studies implicate inflammatory cytokines as gatekeepers for molecular passage within and between tissue compartments of our joints and may open new means to delay the onset and mitigate the progression of degenerative joint diseases such as OA, using pharmaceutical and/or physical measures.
Analysis of contact tracing data showed contribution of asymptomatic and non-severe infections to the maintenance of SARS-CoV-2 transmission in Senegal
Authors: Diarra Maryam , Ndiaye Ramatoulaye , Barry Aliou , Talla Cheikh , Diagne Moussa Moise , Dia Ndongo , Faye Joseph , Sarr Fatoumata Diene , Gaye Aboubacry , Diallo Amadou , Cisse Mamadou , Dieng Idrissa , Fall Gamou , Tall Adama , Faye Oumar , Faye Ousmane , Sall Amadou A. , Loucoubar Cheikh
Published: June 2023

During the COVID-19 pandemic in Senegal, contact tracing was done to identify transmission clusters, their analysis allowed to understand their dynamics and evolution. In this study, we used information from the surveillance data and phone interviews to construct, represent and analyze COVID-19 transmission clusters from March 2, 2020, to May 31, 2021. In total, 114,040 samples were tested and 2153 transmission clusters identified. A maximum of 7 generations of secondary infections were noted. Clusters had an average of 29.58 members and 7.63 infected among them; their average duration was 27.95 days. Most of the clusters (77.3%) are concentrated in Dakar, capital city of Senegal. The 29 cases identified as super-spreaders, i.e., the indexes that had the most positive contacts, showed few symptoms or were asymptomatic. Deepest transmission clusters are those with the highest percentage of asymptomatic members. The correlation between proportion of asymptomatic and degree of transmission clusters showed that asymptomatic strongly contributed to the continuity of transmission within clusters. During this pandemic, all the efforts towards epidemiological investigations, active case-contact detection, allowed to identify in a short delay growing clusters and help response teams to mitigate the spread of the disease.
The relationships between microbiome diversity and epidemiology in domestic species of malaria-mediated mosquitoes of Korea
Authors: Lee Jeong Hyeon , Kim Hyun-Woo , Mustafa Bilal , Lee Hee Il , Kwon Hyung Wook
Published: June 2023

Microbiota in the mosquito plays an important role in their behavior and vector competence. The composition of their microbiome is strongly influenced by the environment, especially their habitat. The microbiome profiles of adult female Anopheles sinensis mosquitoes from malaria hyperendemic and hypoendemic areas in Republic of Korea were compared using 16S rRNA Illumina sequencing. In different epidemiology groups, the alpha and beta diversity analyses were significant. The major bacterial phylum was Proteobacteria. The most abundant species in the microbiome of hyperendemic mosquitoes were the genera Staphylococcus, Erwinia, Serratia, and Pantoea. Notably, a distinct microbiome profile characterized by the dominance of Pseudomonas synxantha was identified in the hypoendemic area, suggesting a potential correlation between the microbiome profiles and the incidence of malaria cases.
Oral micronized progesterone for perimenopausal night sweats and hot flushes a Phase III Canada-wide randomized placebo-controlled 4 month trial
Authors: Prior Jerilynn C. , Cameron Andrea , Fung Michelle , Hitchcock Christine L. , Janssen Patricia , Lee Terry , Singer Joel
Published: June 2023

This study tested progesterone for perimenopausal hot flush ± night sweat (vasomotor symptom, VMS) treatment. It was a double-blind, randomized trial of 300 mg oral micronized progesterone@bedtime versus placebo for 3-months (m) after a 1-m untreated baseline during 2012/1–2017/4. We randomized untreated, non-depressed, screen- and baseline-eligible by VMS, perimenopausal women (with flow within 1-year), ages 35–58 (n = 189). Participants aged 50 (± SD = 4.6) were mostly White, educated, minimally overweight with 63% in late perimenopause; 93% participated remotely. The 1° outcome was 3rd-m VMS Score difference. Participants recorded VMS number and intensity (0–4 scale)/24 h on a VMS Calendar. Randomization required VMS (intensity 2–4/4) of sufficient frequency and/or ≥ 2/week night sweat awakenings. Baseline total VMS Score (SD) was 12.2 (11.3) without assignment difference. Third-m VMS Score did not differ by therapy (Rate Difference − 1.51). However, the 95% CI [− 3.97, 0.95] P = 0.222, did not exclude 3, a minimal clinically important difference. Women perceived progesterone caused decreased night sweats (P = 0.023) and improved sleep quality (P = 0.005); it decreased perimenopause-related life interference (P = 0.017) without increased depression. No serious adverse events occurred. Perimenopausal night sweats ± hot flushes are variable; this RCT was underpowered but could not exclude a minimal clinically important VMS benefit. Perceived night sweats and sleep quality significantly improved.
34-kDa salivary protein enhances duck Tembusu virus infectivity in the salivary glands of Aedes albopictus by modulating the innate immune response
Authors: Sri-in Chalida , Thontiravong Aunyaratana , Bartholomay Lyric C. , Wechtaisong Wittawat , Thongmeesee Kritsada , Riana Elizabeth , Tiawsirisup Sonthaya
Published: June 2023

Duck Tembusu virus (DTMUV) is an important flavivirus that can be transmitted to poultry via Aedes albopictus bites. Furthermore, humans residing in the DTMUV epidemic area display activated antiviral immune responses to local DTMUV isolates during the pathogenic invasion, thereby raising the primary concern that this flavivirus may be transmitted to humans via mosquito bites. Therefore, we identified the gene AALF004421, which is a homolog of the 34-kDa salivary protein (34 kDa) of Ae. albopictus and studied the salivary protein-mediated enhancement of DTMUV infection in Ae. albopictus salivary glands. We observed that double-stranded RNA-mediated silencing of the 34 kDa in mosquito salivary glands demonstrated that the silenced 34 kDa impaired DTMUV infectivity, similar to inhibition through serine protease. This impairment occurred as a consequence of triggering the innate immune response function of a macroglobulin complement-related factor (MCR). 34-kDa in the salivary gland which had similar activity as a serine protease, results in the abrogation of antimicrobial peptides production and strong enhance DTMUV replication and transmission. Although the function of the 34 kDa in Ae. albopictus is currently unknown; in the present study, we showed that it may have a major role in DTMUV infection in mosquito salivary glands through the suppression of the antiviral immune response in the earliest stages of infection. This finding provides the first identification of a prominently expressed 34 kDa protein in Ae. albopictus saliva that could serve as a target for controlling DTMUV replication in mosquito vectors.
Screening properties of trend tests in genetic association studies
Authors: Jiang Zhenzhen , Guo Hongping , Wang Jinjuan
Published: June 2023

In genome-wide association study, extracting disease-associated genetic variants among millions of single nucleotide polymorphisms is of great importance. When the response is a binary variable, the Cochran-Armitage trend tests and associated MAX test are among the most widely used methods for association analysis. However, the theoretical guarantees for applying these methods to variable screening have not been built. To fill this gap, we propose screening procedures based on adjusted versions of these methods and prove their sure screening properties and ranking consistency properties. Extensive simulations are conducted to compare the performances of different screening procedures and demonstrate the robustness and efficiency of MAX test-based screening procedure. A case study on a dataset of type 1 diabetes further verifies their effectiveness.
Effects of modified sleeper stretch and modified cross-body stretch on upper limb functions and shoulder ROM in tennis players: a randomized trial
Authors: D. Anjupriya , Sudhan M. Aparna , Chandran Shilpa , Nuhmani Shibili , Ahsan Mohammad , Alghadir Ahmad H. , Khan Masood
Published: June 2023

Tennis players often experience posterior shoulder pain due to restricted internal rotation (IR) range of motion (ROM) of the glenohumeral joint. No research has compared the effects of modified sleeper stretch (MSS) versus modified cross-body stretch (MCBS) on tennis players’ upper limb functions and IR ROM. The study aimed to compare the efficacy of modified sleeper and cross-body adduction stretch in improving shoulder IR ROM and upper limb functions in tennis players. Thirty male lawn tennis players (aged 20 to 35 years) with more than 15° glenohumeral IR deficiency on the dominant side compared to the non-dominant side were recruited and divided into two groups: Modified sleeper stretch group (MSSG) and modified cross-body stretch group (MCBSG). MSSG received MSS, and MCBSG received MCBS, 3–5 repetitions once daily for 4 weeks. Upper limb functions were measured using the Disability of the Arm, Shoulder, and Hand (DASH) scale, and the IR ROM of the shoulder joint was measured using a universal goniometer. Both groups observed significant (p < 0.05) DASH scores and IR ROM improvements. DASH scores decreased by 85% in MSSG and 79.60% in MCBSG. IR ROM increased by 94.64% in MSSG and 89.52% in MCBSG. No significant differences (p > 0.05) were found in post-intervention DASH scores and IR ROM values between both groups. MSS and MCBS improved upper limb functions and IR ROM of the shoulder joint in the selected sample population of lawn tennis players. No difference was observed between both stretching techniques in improving upper limb functions and IR ROM of the shoulder joint.