bluuebunny/crossref_metadata_2025 huggingface dataset:
Case report: Endoscopic endonasal transposterior clinoid approach for resection of posterior clinoid process meningioma: technical notes and literature review
Authors: Awyono Steven , Takeuchi Kazuhito , Ito Eiji , Nagata Yuichi , Golden Nyoman , Mahadewa Tjokorda Gde Bagus , Saito Ryuta
Published: June 2024

Background

Posterior clinoid process (PCP) meningioma is an exceedingly rare entity. It remains the most challenging skull base lesion for neurosurgeons due to its treacherous location that insinuates amongst critical neurovascular structures. This article will describe the technical notes using the endoscopic endonasal approach that provide the earliest devascularization and detachment of the tumor PCP meningioma.

Methods

We are introducing the surgical implementation of an endoscopic endonasal approach to removing PCP meningioma. Furthermore, we perform a literature review of posterior clinoid process meningioma that undergoes surgical intervention, then summarize the benefits and limitations of each approach.

Results

We present a case of right PCP meningioma that was removed using an endoscopic endonasal approach through the transposterior clinoid corridor in a 52-year-old-woman. We describe the technical notes in performing this approach to have the earliest devascularization and detachment of the tumor by performing posterior clinoidectomy. Safe tumor removal is performed with a wide and clear view of the surrounding neurovascular structure. Based on our database search, we found nine articles reported on the surgical management of PCP meningiomas, with a total number of 15 cases. All of the reported cases performed the tumor removal using the transcranial approach.

Conclusion

The endoscopic endonasal transposterior clinoid approach circumvents all disadvantages faced by the traditional transcranial approach, providing the earliest approach to devascularized and detaching the tumor from its attachment at PCP. This approach demonstrates safety and efficacy, making it an acceptable alternative for PCP meningioma resections.
Safety, tolerability, pharmacokinetics, and pharmacodynamics of a soluble guanylate cyclase stimulator, HEC95468, in healthy volunteers: a randomized, double-blinded, placebo-controlled phase 1 trial
Authors: Gui Yu-zhou , Wang Wei , Wu Qing-qing , Ding Qi-chen , Qian Hong-jie , Lu Qiu-bei , Zhang Ying-jun , Zhuang Yu-lei , Deng Li , Zuo Ying-lin , Luo Lin , Jia Jing-ying
Published: June 2024

Heart failure is the most costly cardiovascular disorder. New treatments are urgently needed. This study aims to evaluate the safety, pharmacokinetics, and pharmacodynamic profile of HEC95468, a soluble guanylate cyclase (sGC) stimulator, in healthy volunteers. Sixty-two, eighteen, and forty-eight participants were enrolled in the single ascending dose (SAD) study, the food effect (FE) study, and the multiple ascending dose (MAD) study, respectively. The study conforms to good clinical practice and the Declaration of Helsinki. Overall, HEC95468 was safe and tolerable; a higher proportion of HEC95468-treated participants reported mild headaches, dizziness, decreased blood pressure, increased heart rate, and gastrointestinal-related treatment-emergent adverse events (TEAEs), similar to the sGC stimulators riociguat and vericiguat. In terms of pharmacokinetic parameters, the maximum observed plasma concentration (Cmax) and the area under the concentration-time curve (AUC0-t) were dose-proportional over the dose range. Moderate accumulation was observed after multiple administrations of HEC95468. Systolic blood pressure (SBP) and diastolic blood pressure decreased, while 3′,5′-cyclic guanosine monophosphate (cGMP) concentration in plasma increased and heart rate was induced. Vasoactive hormones (renin, angiotensin II, and norepinephrine) in plasma were compensatorily elevated after oral administration. These data supported further clinical trials of HEC95468 in the treatment of heart failure and pulmonary arterial hypertension.

Systematic Review Registration:http://www.chinadrugtrials.org.cn, identifier CTR20210064.
Hawthorn with “homology of medicine and food”: a review of anticancer effects and mechanisms
Authors: Zhou Ziying , Nan Yi , Li Xiangyang , Ma Ping , Du Yuhua , Chen Guoqing , Ning Na , Huang Shicong , Gu Qian , Li Weiqiang , Yuan Ling
Published: June 2024

Over the past few years, there has been a gradual increase in the incidence of cancer, affecting individuals at younger ages. With its refractory nature and substantial fatality rate, cancer presents a notable peril to human existence and wellbeing. Hawthorn, a medicinal food homology plant belonging to the Crataegus genus in the Rosaceae family, holds great value in various applications. Due to its long history of medicinal use, notable effects, and high safety profile, hawthorn has garnered considerable attention and plays a crucial role in cancer treatment. Through the integration of modern network pharmacology technology and traditional Chinese medicine (TCM), a range of anticancer active ingredients in hawthorn have been predicted, identified, and analyzed. Studies have shown that ingredients such as vitexin, isoorientin, ursolic acid, and maslinic acid, along with hawthorn extracts, can effectively modulate cancer-related signaling pathways and manifest anticancer properties via diverse mechanisms. This review employs network pharmacology to excavate the potential anticancer properties of hawthorn. By systematically integrating literature across databases such as PubMed and CNKI, the review explores the bioactive ingredients with anticancer effects, underlying mechanisms and pathways, the synergistic effects of drug combinations, advancements in novel drug delivery systems, and ongoing clinical trials concerning hawthorn’s anticancer properties. Furthermore, the review highlights the preventive health benefits of hawthorn in cancer prevention, offering valuable insights for clinical cancer treatment and the development of TCM with anticancer properties that can be used for both medicinal and edible purposes.
The efficacy and safety of Xiao-Ban-Xia-Tang in the treatment of chemotherapy-induced nausea and vomiting: A systematic review and meta-analysis
Authors: Li Ling , Jia Shangmei , Yu Chenghao , Shi Shasha , Peng Fu
Published: June 2024

Background

Chemotherapy-induced nausea and vomiting (CINV) is one of the most frequent and critical side effects due to chemotherapeutics. In China, Xiao-Ban-Xia-Tang (XBXT) has already been applied extensively to prevent and treat CINV. However, there is limited testimony on the effectiveness and safety of this purpose, and there was no correlative systematic review. The aim of this review was to systematically evaluate the effectiveness and safety of XBXT in preventing and treating CINV.

Methods

The systematic search was conducted in eight databases to acquire randomized controlled trials (RCTs) that appraised the effect of XBXT in treating CINV. The vomiting and nausea relief efficiency, eating efficiency, quality of life, and adverse reactions were explored for efficacy assessment. Bias risk was rated by manipulating the Cochrane risk of bias tool 2.0 (RoB 2). The retrieved investigations were analyzed by utilizing ReviewManager 5.4 and Stata 17.0. The quality of evidence was evaluated adopting the GRADE tool.

Results

A total of 16 clinical RCTs of XBXT in the treatment of CINV were incorporated into the investigation, with a total of 1246 participants. The meta-analysis showed that compared with conventional antiemetic drugs, XBXT and antiemetics improved the vomiting relief efficiency (RR 1.35, 95% confidence interval: 1.25–1.46, p < 0.00001), nausea relief efficiency (N = 367, RR 1.23, 95% CI: 1.09–1.38, p < 0.00001), and quality of life (RR = 1.37, 95% CI: 1.14–1.65, p = 0.0009) and reduced the adverse events (N = 370, RR 0.53, 95% CI: 0.29–0.96, p = 0.04). XBXT and DARAs raised eating efficiency compared with DARAs (N = 208, RR 1.30, 95% CI: 1.07–1.57, p = 0.007). The data existed as statistically significant, and the publication bias was identified as relatively low from the funnel plot and trim and fill analysis. In addition, sensitivity analysis demonstrated robust outcomes. The quality of evidence for each outcome ranged from moderate to high.

Conclusion

There is some encouraging evidence that XBXT and antiemetics had better therapeutic effects and safety in treating CINV than antiemetic drugs alone. The quality assessment and low publication bias indicated that the overall criterion was scientific. Better research is required to verify the evidence designed with large-scale RCTs and rigorous methods.

Systematic Review Registration:

https://www.crd.york.ac.uk/PROSPERO/display_record.php?RecordID=281046.
In silico approaches for drug repurposing in oncology: a scoping review
Authors: Cavalcante Bruno Raphael Ribeiro , Freitas Raíza Dias , Siquara da Rocha Leonardo de Oliveira , Santos Roberto de Souza Batista Dos , Souza Bruno Solano de Freitas , Ramos Pablo Ivan Pereira , Rocha Gisele Vieira , Gurgel Rocha Clarissa Araújo
Published: June 2024

Introduction: Cancer refers to a group of diseases characterized by the uncontrolled growth and spread of abnormal cells in the body. Due to its complexity, it has been hard to find an ideal medicine to treat all cancer types, although there is an urgent need for it. However, the cost of developing a new drug is high and time-consuming. In this sense, drug repurposing (DR) can hasten drug discovery by giving existing drugs new disease indications. Many computational methods have been applied to achieve DR, but just a few have succeeded. Therefore, this review aims to show in silico DR approaches and the gap between these strategies and their ultimate application in oncology.

Methods: The scoping review was conducted according to the Arksey and O’Malley framework and the Joanna Briggs Institute recommendations. Relevant studies were identified through electronic searching of PubMed/MEDLINE, Embase, Scopus, and Web of Science databases, as well as the grey literature. We included peer-reviewed research articles involving in silico strategies applied to drug repurposing in oncology, published between 1 January 2003, and 31 December 2021.

Results: We identified 238 studies for inclusion in the review. Most studies revealed that the United States, India, China, South Korea, and Italy are top publishers. Regarding cancer types, breast cancer, lymphomas and leukemias, lung, colorectal, and prostate cancer are the top investigated. Additionally, most studies solely used computational methods, and just a few assessed more complex scientific models. Lastly, molecular modeling, which includes molecular docking and molecular dynamics simulations, was the most frequently used method, followed by signature-, Machine Learning-, and network-based strategies.

Discussion: DR is a trending opportunity but still demands extensive testing to ensure its safety and efficacy for the new indications. Finally, implementing DR can be challenging due to various factors, including lack of quality data, patient populations, cost, intellectual property issues, market considerations, and regulatory requirements. Despite all the hurdles, DR remains an exciting strategy for identifying new treatments for numerous diseases, including cancer types, and giving patients faster access to new medications.
Nicotinamide N-methyltransferase (NNMT): a novel therapeutic target for metabolic syndrome
Authors: Sun Wei-Dong , Zhu Xiao-Juan , Li Jing-Jing , Mei Ya-Zhong , Li Wen-Song , Li Jiang-Hua
Published: June 2024

Metabolic syndrome (MetS) represents a constellation of metabolic abnormalities, typified by obesity, hypertension, hyperglycemia, and hyperlipidemia. It stems from intricate dysregulations in metabolic pathways governing energy and substrate metabolism. While comprehending the precise etiological mechanisms of MetS remains challenging, evidence underscores the pivotal roles of aberrations in lipid metabolism and insulin resistance (IR) in its pathogenesis. Notably, nicotinamide N-methyltransferase (NNMT) has recently surfaced as a promising therapeutic target for addressing MetS. Single nucleotide variants in the NNMT gene are significantly correlated with disturbances in energy metabolism, obesity, type 2 diabetes (T2D), hyperlipidemia, and hypertension. Elevated NNMT gene expression is notably observed in the liver and white adipose tissue (WAT) of individuals with diabetic mice, obesity, and rats afflicted with MetS. Knockdown of NNMT elicits heightened energy expenditure in adipose and hepatic tissues, mitigates lipid accumulation, and enhances insulin sensitivity. NNMT catalyzes the methylation of nicotinamide (NAM) using S-adenosyl-methionine (SAM) as the donor methyl group, resulting in the formation of S-adenosyl-l-homocysteine (SAH) and methylnicotinamide (MNAM). This enzymatic process results in the depletion of NAM, a precursor of nicotinamide adenine dinucleotide (NAD+), and the generation of SAH, a precursor of homocysteine (Hcy). Consequently, this cascade leads to reduced NAD+levels and elevated Hcy levels, implicating NNMT in the pathogenesis of MetS. Moreover, experimental studies employing RNA interference (RNAi) strategies and small molecule inhibitors targeting NNMT have underscored its potential as a therapeutic target for preventing or treating MetS-related diseases. Nonetheless, the precise mechanistic underpinnings remain elusive, and as of yet, clinical trials focusing on NNMT have not been documented. Therefore, further investigations are warranted to elucidate the intricate roles of NNMT in MetS and to develop targeted therapeutic interventions.
Mitochondrial metabolism regulation and epigenetics in hypoxia
Authors: Laird Madison , Ku Jennifer C. , Raiten Jacob , Sriram Sashwat , Moore Megan , Li Yong
Published: June 2024

The complex and dynamic interaction between cellular energy control and gene expression modulation is shown by the intersection between mitochondrial metabolism and epigenetics in hypoxic environments. Poor oxygen delivery to tissues, or hypoxia, is a basic physiological stressor that sets off a series of reactions in cells to adapt and endure oxygen-starved environments. Often called the “powerhouse of the cell,” mitochondria are essential to cellular metabolism, especially regarding producing energy through oxidative phosphorylation. The cellular response to hypoxia entails a change in mitochondrial metabolism to improve survival, including epigenetic modifications that control gene expression without altering the underlying genome. By altering the expression of genes involved in angiogenesis, cell survival, and metabolism, these epigenetic modifications help cells adapt to hypoxia. The sophisticated interplay between mitochondrial metabolism and epigenetics in hypoxia is highlighted by several important points, which have been summarized in the current article. Deciphering the relationship between mitochondrial metabolism and epigenetics during hypoxia is essential to understanding the molecular processes that regulate cellular adaptation to reduced oxygen concentrations.
Reliability and validation of the Japanese version of the cognitive distortion scale
Authors: Takeda Tomoya , Fukudome Koudai , Nakano Mina , Umehara Hidehiro , Nakamura Kimiya
Published: June 2024

The cognitive distortion scale (CDS) is a self-rated measure to assess the degree of cognitive distortion which is 10 thinking errors commonly seen in depression. However, there is no scale to measure 10 types cognitive distortions specific to depression in Japan. Therefore, this study translated the CDS into Japanese (CDS-J), and examined its factor structure, validity, and reliability in a Japanese population. A total of 237 healthy individuals and 39 individuals with depression participated in this study. Confirmatory factor analysis indicated the appropriateness of the CDS-J’s 10-factor structure. Regarding convergent validity, CDS-J was significantly correlated with dysfunctional attitudes, negative automatic thoughts, and depression. Regarding discriminant validity, the CDS-J showed no significant correlation with positive automatic thoughts. The total CDS-J scores of the healthy participants and of those with major depression were compared. The results showed significant differences between groups. Finally, the CDS-J was found to have a high test–retest reliability. Therefore, the CDS-J is a valid and reliable tool for assessing cognitive distortions in Japan.
Visuospatial perspective-taking of a protagonist during narrative comprehension: the effects of task load and individual differences in visuospatial working memory
Authors: Hosokawa Asako , Kitagami Shinji
Published: June 2024

Introduction

This study examined whether visuospatial perspective uses the character perspective during narrative comprehension.

Method

Participants read narrative stimuli depicting the spatial positional relationships between characters and objects and judged whether the objects were on the left or right from the character's perspective. We manipulated whether the spatial positional relationships between characters depicted in the narrative stimuli resulted in a visuospatial perspective. We hypothesized that the high-load perspective-taking condition would indicate longer reaction times compared to the low-load perspective-taking condition, as shifting perspectives between characters in the high-load condition require more time for visuospatial perspective-taking.

Results

As predicted, the reaction time was longer for high-load perspective-taking than for low-load perspective-taking.

Discussion

During narrative comprehension, the reaction time for visuospatial perspective-taking must move virtually within the representation from the main character's perspective to that of another character. Visuospatial perspective-taking is involved in narrative comprehension.
Validity and utility of the Japanese version of the brief unhelpful thoughts and beliefs about stuttering scale: UTBAS-6-J
Authors: Tomisato Shuta , Yada Yasuto , Wasano Koichiro , Kono Takeyuki , Ozawa Hiroyuki
Published: June 2024

Do adults who stutter have abnormally high social anxiety? Is it related to maladaptive cognition? As these are persistent, unresolved questions in stuttering research, it behooves clinicians to at least assess and attempt to identify social anxiety in patients who stutter and its basis before decisions are made about stuttering treatment. The Unhelpful Thoughts and Beliefs About Stuttering (UTBAS) scale is a self-administered questionnaire that measures the degree of non-adaptive cognition in people who stutter (PWS) due to social anxiety. The 66-item UTBAS is time-consuming to complete, prompting the development of a shorter 6-item version, the UTBAS-6, which is in English. Here, we aimed to assess some psychometric properties of the Japanese version of the UTBAS-6, the UTBAS-6-J, which has not been done to date. In 56 adult patients (mean 32.6 ± 11.1 years) who stutter, we quantified the reliability, the internal consistency, and the concurrent validity of the UTBAS-6-J. Along with the UTBAS-6-J, patients also were administered the Overall Assessment of the Speaker’s Experience of Stuttering – Japanese version (OASES-A-J), the Modified Erickson Communication Attitude Scale – Japanese version (S-24-J), and the Liebowitz Social Anxiety Scale – Japanese version (LSAS-J). Cronbach’s alpha for UTBAS-6-J total scores was 0.974, indicating excellent internal consistency. UTBAS-6-J scores were significantly correlated with scores on the OASES-A-J, the S-24-J, and the LSAS-J (all p < 0.005). Concurrent validity of the UTBAS-6-J with these three questionnaires was confirmed. The UTBAS-6-J has good internal consistency and concurrent validity, which will aid clinical decision-making about stuttering treatments.
Investigating the trajectory of post-COVID impairments: a longitudinal study in Sweden
Authors: Badinlou Farzaneh , Abzhandadze Tamar , Rahimian Fatemeh , Jansson-Fröjmark Markus , Hedman-Lagerlöf Maria , Lundgren Tobias
Published: June 2024

Introduction

Individuals recovering from COVID-19 often experience a range of post-recovery symptoms. However, the literature on post-COVID-19 symptoms reveals conflicting results, necessitating a heightened focus on longitudinal studies to comprehend the trajectory of impairments over time. Our study aimed to investigate changes in long-term impairments among individuals infected with COVID-19 and explore potential predictors influencing these changes.

Methods

We conducted a web-survey targeting individuals that had been infected with COVID-19 at four time-points: T0 (baseline), T1 (three months), T2 (six months), and T3 (twelve months). The survey included contextual factors, factors related to body functions and structures, and post-COVID impairments. The longitudinal sample included 213 individuals (with a mean age of 48.92 years). Linear mixed models were employed to analyze changes in post-COVID impairments over time and identify impacting factors.

Results

Findings revealed a general decline in post-COVID impairments over time, with each symptom exhibiting a dynamic pattern of fluctuations. Factors such as initial infection severity, education level, and work status were significantly associated with the levels of impairments.

Discussion

The study emphasizes that post-COVID impairments are not static but exhibit variations over time. Personalized care, especially for vulnerable populations, is crucial. The results underscore the need for long-term monitoring and multidisciplinary treatment approaches. Targeted support and interventions are highlighted for individuals with severe initial infections and those in socioeconomically disadvantaged groups.
Nicotine and fluoxetine alter adolescent dopamine-mediated behaviors via 5-HT1A receptor activation
Authors: Yuan Menglu , Leslie Frances M.
Published: June 2024

Introduction

Abuse or misuse of tobacco, e-cigarettes, or antidepressants may have serious clinical consequences during adolescence, a sensitive period during brain development when the distinct neurobiology of adolescent serotonin (5-HT) and dopamine (DA) systems create unique behavioral vulnerabilities to drugs of abuse.

Methods

Using a pharmacological approach, we modeled the behavioral and neurochemical effects of subchronic (4-day) nicotine (60µg/kg, i.v.) or fluoxetine (1mg/kg, i.v.) exposure in adolescent and adult male rats.

Results

Nicotine and fluoxetine significantly enhance quinpirole-induced locomotor activity and initial cocaine self-administration in adolescents, but not adults. These effects were blocked by serotonin 5-HT1A receptor antagonists, WAY-100,635 (100 µg/kg, i.v.) or S-15535 (300 µg/kg, i.v.). Neurochemical and anatomical autoradiographic analysis of 8-OH-DPAT-stimulated [35S]GTPγS reveal that prior exposure to nicotine and fluoxetine results in both overlapping and distinct effects on regional 5-HT1A receptor activity. Both fluoxetine and nicotine enhance adolescent 5-HT1A receptor activity in the primary motor cortex (M1), whereas fluoxetine alone targets prefrontal cortical neurocircuitry and nicotine alone targets the amygdala.

Discussion

Given their different pharmacological profiles, comparison between WAY-100,635 and S-15535 indicates that postsynaptic 5-HT1A receptors mediate the behavioral effects of prior nicotine and fluoxetine exposure. In addition, within the adolescent M1, maladaptive changes in 5-HT signaling and 5-HT1A activity after nicotine or fluoxetine exposure may potentiate hyper-responsiveness to dopaminergic drugs and prime adolescent vulnerability for future substance abuse.
Re-assessment of schistosomiasis in nine endemic districts with cluster sampling in Sierra Leone
Authors: Kargbo-Labour Ibrahim , Redwood-Sawyerr Victoria , Conteh Abdulai , Chop Elisabeth , Bah Mohamed S. , Koroma Abdulai , Kabia Unidiatu , Klein Luke , Juneja Sugandh , Houck Patricia , Reid Steven , Evans Darin , Weaver Angela M. , Phillips Anna E. , Zhang Yaobi
Published: June 2024

Background

Baseline mapping showed that schistosomiasis was highly/moderately endemic in nine districts in Sierra Leone. Mass drug administration (MDA) with praziquantel started in 2009, and after multiple rounds of treatment, an impact assessment was conducted in 2016 followed by a second re-assessment in 2022 using cluster sampling to provide more granular data for refining chiefdom (sub-district) treatment strategies.

Methods

On average, 20 rural villages were systematically selected per district by probability proportional to population size across the nine districts. Surveys were conducted in schools, and 24 school children aged between 5 and 14 years were randomly selected, with an equal number of boys and girls. One stool sample and one urine sample were collected per child. Two Kato-Katz slides were examined per stool for Schistosoma mansoni infection. Hemastix strips were used as a proxy for S. haematobium infection with urine filtration used for egg counts on hematuria-positive samples.

Results

In total, 4,736 stool samples and 4,618 urine samples were examined across 200 schools in 125 chiefdoms. Overall, the prevalence of S. mansoni was 16.3% (95% CI: 15.3–17.4%), while the overall prevalence of S. haematobium was 2.0% (95% CI: 1.6–2.4%) by hematuria. The prevalence of heavy infections for S. mansoni and S. haematobium was 1.5% (95% CI: 1.1–1.9%) and 0.02% (95% CI: 0.0–0.14%), respectively. Among 125 chiefdoms surveyed, the overall schistosomiasis prevalence was <10% in 65 chiefdoms, 10–49.9% in 47 chiefdoms, and ≥ 50% in 13 chiefdoms. There was a mixed relationship between schistosomiasis in school children and WASH access in schools.

Conclusion

Sierra Leone has made significant progress in reducing schistosomiasis prevalence across the country after a decade of MDA intervention. However, high prevalence remains in some hotspot chiefdoms. The next steps are for the national program to investigate and address any potential issues such as low coverage or poor knowledge of schistosomiasis risk behaviors and, where appropriate, consider broadening to community-wide treatment in hotspot chiefdoms or communities.
Unlocking the brain’s zinc code: implications for cognitive function and disease
Authors: Sabouri Soheila , Rostamirad Marzieh , Dempski Robert E.
Published: June 2024

Zn2+ transport across neuronal membranes relies on two classes of transition metal transporters: the ZnT (SLC30) and ZIP (SLC39) families. These proteins function to decrease and increase cytosolic Zn2+ levels, respectively. Dysfunction of ZnT and ZIP transporters can alter intracellular Zn2+ levels resulting in deleterious effects. In neurons, imbalances in Zn2+ levels have been implicated as risk factors in conditions such as Alzheimer’s disease and neurodegeneration, highlighting the pivotal role of Zn2+ homeostasis in neuropathologies. In addition, Zn2+ modulates the function of plasma membrane proteins, including ion channels and receptors. Changes in Zn2+ levels, on both sides of the plasma membrane, profoundly impact signaling pathways governing cell development, differentiation, and survival. This review is focused on recent developments of neuronal Zn2+ homeostasis, including the impact of Zn2+ dyshomeostasis in neurological disorders, therapeutic approaches, and the increasingly recognized role of Zn2+ as a neurotransmitter in the brain.
Exploring the influence of internet technology adoption on the technical efficiency of food production: insight from wheat farmers
Authors: Ahmad Bilal , Zhao Zixiang , Jile Xing , Gultaj Humaira , Khan Nawab , Yunxian Yan
Published: June 2024

Digital technology plays a crucial role in advancing sustainable farming and ensuring food security, especially in developing countries. This study evaluates the impact of Internet technology usage on technical efficiency in crop productivity, using data from 600 wheat farmers in rural Pakistan. It addresses the imperative need to enhance agricultural practices within the context of sustainable food production. To achieve this, a matched sample of Internet users and non-users was formed through propensity score matching. The study employs the stochastic frontier method with sample selection adjustment, ensuring a robust evaluation of technical efficiency between these groups. The findings reveal a positive influence of Internet usage on efficiency, persisting even after mitigating self-selection bias from observed and unobserved factors. Internet users exhibit a technical efficiency score of 0.62, surpassing the 0.55 score of non-users. Quantile regression analysis exposes varying impacts of Internet usage on technical efficiency, with less efficient farmers experiencing substantial improvements. Widespread Internet adoption holds the potential to significantly enhance agricultural production for growers. The research underscores the role of promoting Internet utilization to stimulate growth and improve farming efficiency within the evolving digital economy. Policymakers are advised to promote the adoption of modern technology to enhance crop production and support economic growth.
Detection of antibiotic resistant Enterobacterales in commercial raw pet food: a preliminary study
Authors: Fisher Carolyn D. , Call Douglas R. , Omulo Sylvia
Published: June 2024

Introduction

Raw diets have become popular in companion animal nutrition, but these diets may be contaminated with harmful bacteria because heat processing is not utilized to mitigate pathogens during the production process. We analyzed 24 commercially available frozen raw canine and feline diets for extended-spectrum beta-lactamase-producing Enterobacterales (ESBL-E).

Methods

Samples were incubated in tryptic soy broth augmented with 50 μg/mL ampicillin to enrich for ESBL-E. ESBL-E were isolated using CHROMagar ESBL plates and isolate identification and antibiotic susceptibility testing were confirmed using the VITEK®2 instrument.

Results

ESBL-E were isolated from 42% (10/24) of raw diets, with E. coli, Enterobacter cloacae complex and Klebsiella pneumoniae predominating. Most ESBL-E isolates (71%, 32/45) were multidrug-resistant. Direct plating of samples onto tryptic soy agar yielded bacterial counts >6 log10 for 2 samples from two different manufacturers.

Conclusion

This preliminary study justifies further investigation into the potential contribution of raw diets to the dissemination of antibiotic resistant bacteria in companion animals and domestic living spaces.
Depressive Symptoms Among Higher Education Students in Germany—A Systematic Review and Meta-Analysis
Authors: Heumann Eileen , Palacio Siebe Ana Valentina , Stock Christiane , Heinrichs Katherina
Published: June 2024

Objectives

Higher education students in Germany are vulnerable to depressive symptoms. Despite ample evidence, no comprehensive review has recently been conducted. Thus, our systematic review and meta-analysis aims at describing the extent to which students are affected by depressive symptoms.

Methods

We searched three databases for articles reporting the prevalence rates of depressiveness among students in Germany published between 2002 and 2023. Pooled prevalence rates were calculated using random effects models, both for the overall sample and for subgroups categorized by gender, study setting, assessment instrument, and whether the study was conducted before or during the COVID-19 pandemic.

Results

The search yielded 992 records. After screening, 60 articles remained for data extraction. About one out of five students (21.1%) exhibits depressive symptoms. Pooled prevalence rates differ between subgroups, with higher rates during the COVID-19 pandemic than before (30.6% versus 18.0%) and with females being more affected than their male counterparts (29.0% versus 23.1%).

Conclusion

This review underlines the urgency with which the mental health of students should be addressed at the (higher educational) policy level.

Clinical Trial Registration:

PROPSPERO, Identifier CRD42022384066.
Evaluation of Yield and Yield Components of Rice in Vertical Agro-Photovoltaic System in South Korea
Authors: Jo Hyun , Song Jong Tae , Cho Hyeonjun , Lee Sangyeab , Choi Seungmin , Jung Ho-Jun , Lee Hyeong-No , Lee Jeong-Dong
Published: June 2024

Renewable energy from photovoltaic power plants has increased in amount globally as an alternative energy to combat global climate change by reducing fossil fuel burning and carbon dioxide (CO2) emissions. The agro-photovoltaic (APV) approach can be a solution to produce solar energy and crop production at the same time by installing solar panels on the same farmland to increase land use efficiency. This study aimed to compare the yield and yield components of rice (Oryza sativa L.) between a vertical APV system and a control field across two years. The solar panels were installed around the rice field in four directions of rice cultivation. Based on the analysis of variance, the primary factor influencing measured yield and yield components was the year effect, whereas the direction effect did not show significance, except for amylose content and ripened grains. Especially for rice production, the rice yield in 2023 was 6.8 t/ha, which was significantly higher by 0.8 t/ha than in 2022. Compared with the control condition, however, there was no significant negative impact on the year-to-year rice yield of the vertical APV system across two years. As rice yield was mainly affected by year, rice yield trials will be required for multiple years to understand the genetic and environmental factors influencing rice production under the vertical APV system.
Plant Genetic Resources for Food and Agriculture: The Role and Contribution of CREA (Italy) within the National Program RGV-FAO
Authors: Vaccino Patrizia , Antonetti Maurizio , Balconi Carlotta , Brandolini Andrea , Cappellozza Silvia , Caputo Angelo Raffaele , Carboni Andrea , Caruso Marco , Copetta Andrea , de Dato Giovanbattista , De Vita Pasquale , Fascella Giancarlo , Ferretti Luca , Ficcadenti Nadia , Fusani Pietro , Gardiman Massimo , Giovannini Daniela , Giovinazzi Jessica , Iori Angela , Leogrande Rita , Montalbano Vincenzo , Palombi Maria Antonietta , Pecetti Luciano , Perri Enzo , Petriccione Milena , Sala Tea , Storchi Paolo , Tondelli Alessandro , Tripodi Pasquale , Virzì Nino , Verde Ignazio
Published: June 2024

Conservation, characterization and exploitation of agrobiodiversity are key factors to guarantee food security and face future challenges such as climate changes. These issues are the subject of a series of international agreements, such as the Convention of Biological Diversity, with its Nagoya Protocol, and the International Treaty on Plant Genetic Resources for Food and Agriculture (ITPGRFA) adopted in 2001 and entered into force in 2004. Italy ratified the Treaty in 2004 and instituted a long-lasting program, RGV-FAO, to implement it. CREA is one of the three organizations involved in the RGV-FAO Program, together with the National Research Council (CNR) and Reti Semi Rurali. CREA maintains a total of 40,186 accessions including cereals, vegetables, fruits, forages, industrial crops, forest and woody crops, medicinal and aromatic plants, and their wild relatives. Accessions are conserved using different ex situ conservation systems (seeds, in vivo plants, vegetative organs and in vitro plantlets), and characterized using genetic, morpho-phenological and/or biochemical methods. Herein, we will present the CREA long-lasting program RGV-FAO with some examples of the use of plant genetic resources in breeding programs, including molecular approaches. Some critical issues related to access and benefit sharing in PGRFA, such as the Nagoya Protocol and the Digital Sequence Information, will be discussed, highlighting their potential impact on food security and on the advancement of knowledge.
Temporal Transcriptome Dynamics of Longissimus dorsi Reveals the Mechanism of the Differences in Muscle Development and IMF Deposition between Fuqing Goats and Nubian Goats
Authors: Liu Yuan , Wu Xianfeng , Xu Qian , Lan Xianyong , Li Wenyang
Published: June 2024

In this study, we measured the growth performance and intramuscular fat (IMF) content of the Longissimus dorsi (LD) of Fuqing goats (FQs) and Nubian goats (NBYs), which exhibit extreme phenotypic differences in terms of their production and meat quality traits. RNA-Seq analysis was performed, and transcriptome data were obtained from the LD tissue of 3-month fetuses (E3), 0-month lambs (0M), 3-month lambs (3M), and 12-month lambs (12M) to reveal the differences in the molecular mechanisms regulating the muscle development and IMF deposition between FQs and NBYs. The results showed that a higher body weight and average daily gain were observed in the NBYs at three developmental stages after birth, whereas a higher IMF content was registered in the FQs at 12M. Additionally, transcriptome profiles during the embryonic period and after birth were completely different for both FQs and NBYs. Moreover, DEGs (KIF23, CCDC69, CCNA2, MKI67, KIF11, RACGAP1, NUSAP1, SKP2, ZBTB18, NES, LOC102180034, CAPN6, TUBA1A, LOC102178700, and PEG10) significantly enriched in the cell cycle (ko04110) at E3 (FQs vs. NBYs), and DEGs (MRPS7, RPS8, RPL6, RPL4, RPS11, RPS10, RPL5, RPS6, RPL8, RPS13, RPS24, RPS15, RPL23) significantly enriched in ribosomes (ko03010) at 0M (FQs vs. NBYs) related to myogenic differentiation and fusion were identified. Meanwhile, the differences in glucose and lipid metabolism began at the E3 timepoint and continued to strengthen as growth proceeded in FQs vs. NBYs. DEGs (CD36, ADIROQR2, ACACA, ACACB, CPT1A, IGF1R, IRS2, LDH-A, PKM, HK2, PFKP, PCK1, GPI, FASN, FADS1, ELOVL6, HADHB, ACOK1, ACAA2, and ACSL4) at 3M (FQs vs. NBYs) and 12M (FQs vs. NBYs) significantly enriched in the AMPK signaling pathway (ko04152), insulin resistance (ko04931), the insulin signaling pathway (ko04910), fatty acid metabolism (ko01212), and glycolysis/gluconeogenesis (ko00010) related to IMF deposition were identified. Further, the results from this study provide the basis for future studies on the mechanisms regulating muscle development and IMF deposition in different breeds of goats, and the candidate genes identified could be used in the selection process.
Nuciferine Protects Cochlear Hair Cells from Ferroptosis through Inhibiting NCOA4-Mediated Ferritinophagy
Authors: Gao Xian , Mao Huanyu , Zhao Liping , Li Xiang , Liao Yaqi , Li Wenyan , Li Huawei , Chen Yan
Published: June 2024

Cisplatin is a widely used antineoplastic drug for treating various types of cancers. However, it can cause severe side effects, such as bilateral and irreversible hearing loss, which significantly impacts quality of life. Ferroptosis, an iron-dependent form of programmed cell death, has been implicated in the pathogenesis of cisplatin-induced ototoxicity. Here, we investigated the effects of nuciferine, a natural active ingredient isolated from lotus species, on the ferroptosis of cochlear hair cells. Firstly, our results demonstrated that nuciferine can protect hair cells against RSL3-induced and cisplatin-induced damage. Secondly, nuciferine treatment reduced ferrous iron (Fe2+) overload in cochlear hair cells via inhibiting NCOA4-mediated ferritinophagy. Inhibition of ferritinophagy by knocking down Ncoa4 alleviated cisplatin-induced ototoxicity. Importantly, nuciferine treatment mitigated cochlear hair cell loss and damage to ribbon synapse, and improved mouse hearing function in an acute cisplatin-induced hearing loss model. Our findings highlight the role of NCOA4-mediated ferritinophagy in the pathogenesis of cisplatin-induced ototoxicity and provide evidence for nuciferine as a promising protective agent for treating cisplatin-induced hearing loss.
β-Cyclocitral from Lavandula angustifolia Mill. Exerts Anti-Aging Effects on Yeasts and Mammalian Cells via Telomere Protection, Antioxidative Stress, and Autophagy Activation
Authors: Shan Jiaheng , Mo Jianxia , An Chenyue , Xiang Lan , Qi Jianhua
Published: June 2024

We used a replicative lifespan (RLS) experiment of K6001 yeast to screen for anti-aging compounds within lavender extract (Lavandula angustifolia Mill.), leading to the discovery of β-cyclocitral (CYC) as a potential anti-aging compound. Concurrently, the chronological lifespan (CLS) of YOM36 yeast and mammalian cells confirmed the anti-aging effect of CYC. This molecule extended the yeast lifespan and inhibited etoposide (ETO)-induced cell senescence. To understand the mechanism of CYC, we analyzed its effects on telomeres, oxidative stress, and autophagy. CYC administration resulted in notable increases in the telomerase content, telomere length, and the expression of the telomeric shelterin protein components telomeric-repeat binding factor 2 (TRF2) and repressor activator protein 1 (RAP1). More interestingly, CYC reversed H2O2-induced telomere damage and exhibited strong antioxidant capacity. Moreover, CYC improved the survival rate of BY4741 yeast under oxidative stress induced by 6.2 mM H2O2, increasing the antioxidant enzyme activity while reducing the reactive oxygen species (ROS), reactive nitrogen species (RNS), and malondialdehyde (MDA) levels. Additionally, CYC enhanced autophagic flux and free green fluorescent protein (GFP) expression in the YOM38-GFP-ATG8 yeast strain. However, CYC did not extend the RLS of K6001 yeast mutants, such as Δsod1, Δsod2, Δcat, Δgpx, Δatg2, and Δatg32, which lack antioxidant enzymes or autophagy-related genes. These findings reveal that CYC acts as an anti-aging agent by modifying telomeres, oxidative stress, and autophagy. It is a promising compound with potential anti-aging effects and warrants further study.
Solving Optimal Electric Vehicle Charger Deployment Problem
Authors: Kim Seungmo , Jeong Yeonho , Nam Jae-Won
Published: June 2024

Electric vehicles (EVs) have already been acknowledged to be the most viable solution to the climate change that the entire globe has long been combating. Along the same line, it is a salient subject to expand the availability of EV charging infrastructure, which quintessentially necessitates the optimization of the charger’s locations. This paper proposes to formulate the optimal EV charger location problem into a facility location problem (FLP). As an effort to find an efficient method to solve the well-known nonpolynomial deterministic (NP) hard problem, we present a comparative quantification among several representative solving techniques. This paper features two comprehensive case studies representing regions with an average and a high density of EVs. As such, this paper shows that the proposed framework can lead to successful location optimization with adequate refinement of solving techniques.
Safety Management and Accident-Control Strategy for a Commercial-Scale Plant for Supercritical Water Oxidation of Sludge
Authors: Yang Jianqiao , Xie Shijing , Wang Shuzhong
Published: June 2024

Supercritical water oxidation is a promising technology for decomposition of industrial wastewater and sludge. However, the system is operated under high temperature and pressure (usually higher than 500 °C and 25 MPa). Corrosion of component materials and salt deposition may lead to leakage or even the burst of the pressure vessel in the SCWO system, resulting in a high level of worry about the safe operation of the system. In this paper, the safety management and accident- control strategies are introduced according to a commercial-scale SCWO plant in China. The safety management strategy refers to the special design and operation strategies of some facilities. Different types of potential accidents are analyzed and the coping strategies for accidents of different levels of severity are described in detail. The strategies are valuable for the safe operation of commercial SCWO plants and other plants operated in high temperature and high pressure.
Repair and DNA Polymerase Bypass of Clickable Pyrimidine Nucleotides
Authors: Endutkin Anton V. , Yudkina Anna V. , Zharkov Timofey D. , Barmatov Alexander E. , Petrova Daria V. , Kim Daria V. , Zharkov Dmitry O.
Published: June 2024

Clickable nucleosides, most often 5-ethynyl-2′-deoxyuridine (EtU), are widely used in studies of DNA replication in living cells and in DNA functionalization for bionanotechology applications. Although clickable dNTPs are easily incorporated by DNA polymerases into the growing chain, afterwards they might become targets for DNA repair systems or interfere with faithful nucleotide insertion. Little is known about the possibility and mechanisms of these post-synthetic events. Here, we investigated the repair and (mis)coding properties of EtU and two bulkier clickable pyrimidine nucleosides, 5-(octa-1,7-diyn-1-yl)-U (C8-AlkU) and 5-(octa-1,7-diyn-1-yl)-C (C8-AlkC). In vitro, EtU and C8-AlkU, but not C8-AlkC, were excised by SMUG1 and MBD4, two DNA glycosylases from the base excision repair pathway. However, when placed into a plasmid encoding a fluorescent reporter inactivated by repair in human cells, EtU and C8-AlkU persisted for much longer than uracil or its poorly repairable phosphorothioate-flanked derivative. DNA polymerases from four different structural families preferentially bypassed EtU, C8-AlkU and C8-AlkC in an error-free manner, but a certain degree of misincorporation was also observed, especially evident for DNA polymerase β. Overall, clickable pyrimidine nucleotides could undergo repair and be a source of mutations, but the frequency of such events in the cell is unlikely to be considerable.
The Long Journey from Animal Electricity to the Discovery of Ion Channels and the Modelling of the Human Brain
Authors: Catacuzzeno Luigi , Michelucci Antonio , Franciolini Fabio
Published: June 2024

This retrospective begins with Galvani’s experiments on frogs at the end of the 18th century and his discovery of ‘animal electricity’. It goes on to illustrate the numerous contributions to the field of physical chemistry in the second half of the 19th century (Nernst’s equilibrium potential, based on the work of Wilhelm Ostwald, Max Planck’s ion electrodiffusion, Einstein’s studies of Brownian motion) which led Bernstein to propose his membrane theory in the early 1900s as an explanation of Galvani’s findings and cell excitability. These processes were fully elucidated by Hodgkin and Huxley in 1952 who detailed the ionic basis of resting and action potentials, but without addressing the question of where these ions passed. The emerging question of the existence of ion channels, widely debated over the next two decades, was finally accepted and, a decade later, many of them began to be cloned. This led to the possibility of modelling the activity of individual neurons in the brain and then that of simple circuits. Taking advantage of the remarkable advances in computer science in the new millennium, together with a much deeper understanding of brain architecture, more ambitious scientific goals were dreamed of to understand the brain and how it works. The retrospective concludes by reviewing the main efforts in this direction, namely the construction of a digital brain, an in silico copy of the brain that would run on supercomputers and behave just like a real brain.