bluuebunny/crossref_metadata_2025 huggingface dataset:
Design and Analysis of an O+E-Band Hybrid Optical Amplifier for CWDM Systems
Authors: Kanwal Benish , Armghan Ammar , Ghafoor Salman , Atieh Ahmad , Sajid Muhammad , Kausar Tasleem , Mirza Jawad , Lu Yun
Published: November 2022

Broadband amplification in the O+E-band is very desirable nowadays as a way of coping with increasing bandwidth demands. The main issue with doped fiber amplifiers working in this band such as the bismuth-doped fiber amplifier is that they are costly and not widely available. Therefore, a wideband and flat-gain hybrid optical amplifier (HOA) covering the O+E-band based on a parallel combination of a praseodymium-doped fiber amplifier (PDFA) and a semiconductor optical amplifier (SOA) is proposed and demonstrated through numerical simulations. The praseodymium-doped fiber (PDF) core is pumped using a laser diode with a power of 500 mW that is centered at a wavelength of 1030 nm. The SOA is driven by an injection current of 60 mA. The performance of the HOA is analyzed by the optimization of various parameters such as the PDF length, Pr3+ concentration, pump wavelength, and injection current. A flat average gain of 24 dB with a flatness of 1 dB and an output power of 9.6 dBm is observed over a wavelength range of 1270–1450 nm. The noise figure (NF) varies from a minimum of 4 dB to a maximum of 5.9 dB for a signal power of 0 dBm. A gain reduction of around 4 dB is observed for an O-band signal at a wavelength of 1290 nm by considering the up-conversion effect. The transmission performance of the designed HOA as a pre-amplifier is evaluated based on the bit-error rate (BER) analysis for a coarse wavelength-division multiplexing (CWDM) system of eight on-off keying (OOK)-modulated channels, each having a data rate of 10 Gbps. An error-free transmission over 60 km of standard single-mode fiber (SMF) is achieved for different data rates of 5 Gbps, 7.5 Gbps, and 10 Gbps.
Effect of Annealing Time on the Cyclic Characteristics of Ceramic Oxide Thin Film Thermocouples
Authors: Han Yuning , Ruan Yong , Xue Meixia , Wu Yu , Shi Meng , Song Zhiqiang , Zhou Yuankai , Teng Jiao
Published: November 2022

Oxide thin film thermocouples (TFTCs) are widely used in high-temperature environment measurements and have the advantages of good stability and high thermoelectric voltage. However, different annealing processes affect the performance of TFTCs. This paper studied the impact of different annealing times on the cyclic characteristics of ceramic oxide thin film thermocouples. ITO/In2O3 TFTCs were prepared on alumina ceramics by a screen printing method, and the samples were annealed at different times. The microstructure of the ITO film was studied by scanning electron microscopy (SEM), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS). The results show that when the annealing temperature is fixed, the stability of the thermocouple is worst when it is annealed for 2 h. Extending the annealing time can improve the properties of the film, increase the density, slow down oxidation, and enhance the thermal stability of the thermocouple. The thermal cycle test results show that the sample can reach five temperature rise and fall cycles, more than 50 h, and can meet the needs of stable measurement in high temperature and harsh environments.
Role of Genital Tract Bacteria in Promoting Endometrial Health in Cattle
Authors: Adnane Mounir , Chapwanya Aspinas
Published: November 2022

Microbiota regulate endometrial health in cattle. It is important to know what a ‘good’ microbiome is, in order to understand pathogeneses of uterine disease. Given that microbial influx into the genital tract of cows at calving is unavoidable, exploring the involvement of genital tract bacteria in promoting endometrial health is warranted. The dysbiosis of endometrial microbiota is associated with benign and malign uterine diseases. The present review discusses current knowledge about the altered endometrial microbiome and the implications of this modulation on endometrial inflammation, ovarian activity, fecundation, pregnancy, and postpartum complications. Intravaginal administration of symbiotic microbes in cattle is a realistic alternative to antibiotic and hormone therapy to treat uterine disease. Genital microbial diversity can be modeled by nutrition, as the energy balance would improve the growth of specific microbial populations. It may be that probiotics that alter the endometrial microbiome could provide viable alternatives to existing therapies for uterine disease in cattle.
A Rationale and Approach to the Development of Specific Treatments for HIV Associated Neurocognitive Impairment
Authors: Scanlan Aaron , Zhang Zhan , Koneru Rajeth , Reece Monica , Gavegnano Christina , Anderson Albert M. , Tyor William
Published: November 2022

Neurocognitive impairment (NCI) associated with HIV infection of the brain impacts a large proportion of people with HIV (PWH) regardless of antiretroviral therapy (ART). While the number of PWH and severe NCI has dropped considerably with the introduction of ART, the sole use of ART is not sufficient to prevent or arrest NCI in many PWH. As the HIV field continues to investigate cure strategies, adjunctive therapies are greatly needed. HIV imaging, cerebrospinal fluid, and pathological studies point to the presence of continual inflammation, and the presence of HIV RNA, DNA, and proteins in the brain despite ART. Clinical trials exploring potential adjunctive therapeutics for the treatment of HIV NCI over the last few decades have had limited success. Ideally, future research and development of novel compounds need to address both the HIV replication and neuroinflammation associated with HIV infection in the brain. Brain mononuclear phagocytes (MPs) are the primary instigators of inflammation and HIV protein expression; therefore, adjunctive treatments that act on MPs, such as immunomodulating agents, look promising. In this review, we will highlight recent developments of innovative therapies and discuss future approaches for HIV NCI treatment.
Production Decline Analysis of Tight Conglomerate Reservoirs with Small Well Spacing, Based on the Fractal Characteristics of Fracture Networks
Authors: Li Xiaoshan , Song Junqiang , Pan Hong , Gu Kaifang , Wang Shuo , Yang Liu , You Haoyu , Wang Li , Wang Xin , Li Shihong , Li Ting , Luo Guanxing
Published: November 2022

The conglomerate matrix and fracture propagation are special in tight conglomerate reservoir with small well spacing. In this article, the fractal propagation characteristics of the fracture network in conglomerate reservoirs are described by experiment and a micro-mathematical model. According to the core slice, the conglomerate reservoir matrix presents the multi-modal pore structure, described as the “pseudo-dual-media” model. Given the above, the unsteady seepage mathematical model, comprehensively considering the fractal fracture network, stress sensitivity of main fractures, and threshold pressure gradient of the reservoir matrix, was developed and analytically solved. The Blasingame type curves for production decline analysis were plotted, and the sensitive parameters were analyzed. The field application was performed for validation. The research results show that the fractal dimension decides the complexity of the fracture network distribution. As it increases, the unsteady flow occurs earlier, and the boundary flow is delayed. The anomalous diffusion exponent represents the smoothness of crude oil migration and a higher value leads to higher resistance to oil migration and larger pressure drawdown for the same production rate. The growth of the threshold pressure gradient within a certain range can result in a localized downward shift of the type curves. The field application in a conglomerate oil reservoir showed that the presented model presents a fitting accuracy 10% higher than that of the conventional SRV model and has high reliability and precision for the production performance evaluation of the small-well-spacing development of tight conglomerate reservoirs.
Prediction of Sulfur Removal from Iron Concentrate Using Column Flotation Froth Features: Comparison of k-Means Clustering, Regression, Backpropagation Neural Network, and Convolutional Neural Network
Authors: Nakhaei Fardis , Rahimi Samira , Fathi Mohammadbagher
Published: November 2022

Froth feature extraction plays a significant role in the monitoring and control of the flotation process. Image-based soft sensors have received a great deal of interest in the flotation process due to their low-cost and non-intrusive properties. This study proposes data-driven soft sensor models based on froth images to predict the key performance indicators of the flotation process. The ability of multiple linear regression (MLR), the backpropagation neural network (BPNN), the k-means clustering algorithm, and the convolutional neural network (CNN) to predict the amount of sulfur removal from iron ore concentrate in the column flotation process was examined. A total of 99 experimental results were used to develop the predictive models. Extracted froth features including color, bubble shape and size, texture, stability, and velocity were used to train the traditional predictive models, whereas in the CNN model the froth images were directly fed into the model. The results comparison indicated that the three-layered feedforward NN model (17-10-1 topology) and CNN model provided better predictions than the MLR and k-means algorithm. The BPNN model displayed a correlation coefficient of 0.97 and a root mean square error of 4.84% between the actual data and network output for both training and the testing datasets. The error percentages of the CNN, BPNN, MLR and k-means models were 10, 11, 15 and 18%, respectively. This study can become a key technical support for the application of intelligent models in the control of the operational variables for the flotation process used to desulfurize iron concentrate.
Trace Elements in Chromian Spinels from Four Siberian Kimberlites
Authors: Venier Marco , Ziberna Luca , Princivalle Francesco , Petrelli Maurizio , Lughi Vanni , Logvinova Alla , Sobolev Nikolay V. , Turco Gianluca , Lenaz Davide
Published: November 2022

We analysed the major, minor and trace elements chemistry of forty-two Cr-spinels from four Siberian kimberlites. They showed a wide range in Mg# (Mg/(Mg + Fe2+); 0.42–0.78) and Cr# (Cr/(Cr + Al); 0.32–0.92) and a common trend of increasing Cr# with decreasing Mg#. The major element classification schemes suggested that there were spinels deriving from a peridotitic source (Xen) and spinels crystallised from kimberlitic melts (Chr). Laser-Ablation Inductively Coupled Plasma Mass Spectrometry on both groups showed that the trace elements with the highest abundance were Mn (985–3390 ppm), Ni (531–3162 ppm), V (694–2510 ppm) and Zn (475–2230 ppm). Testing the effectiveness of trace elements in determining the source for Cr-spinels, we found out that Cr-spinels crystallised directly from a kimberlitic melt usually showed higher Mn, Ni, Sc and V concentrations with respect to those of peridotitic origin. In addition, using the available partitioning models, we found that the correlations between major elements and Ni, Co, Sc and Ga in the Xen group could be explained by subsolidus equilibration between spinel, olivine and clinopyroxene at 800–1000 °C, thus supporting a peridotitic source for this group. Finally, we calculated the composition of the possible melts in equilibrium with the Cr-spinels of the Chr group, using a selected set of partition coefficients. Calculated abundances of Cu, Ga and Zr were comparable to those of the kimberlite, while V was never close to the kimberlite composition. This simulation highlighted the need for new data on the trace elements partition coefficients between kimberlitic melts and Cr-spinel.
Rapid Microwave-Assisted Synthesis of N/TiO2/rGO Nanoparticles for the Photocatalytic Degradation of Pharmaceuticals
Authors: Sanchez Tobon Camilo , Panžić Ivana , Bafti Arijeta , Matijašić Gordana , Ljubas Davor , Ćurković Lidija
Published: November 2022

Nanocomposites comprising nitrogen-doped TiO2 and reduced graphene oxide (N/TiO2/rGO), with different rGO loading qualities, were prepared by a cost-effective microwave-assisted synthesis method. The synthesized materials were broadly characterized by Raman spectroscopy, X-ray diffraction (XRD), infrared spectroscopy (FTIR), photoelectron spectroscopy (XPS), diffuse reflectance spectroscopy (DRS), electron microscopy (SEM-EDS), and nitrogen adsorption/desorption isotherms. Anatase was the only crystalline phase observed for all synthesized materials. The rGO loading did not affect the morphological properties, but it positively influenced the photocatalytic activity of the nanocomposite materials, especially at low rGO loading. Photocatalysts were evaluated via the degradation of specific organic micropollutant (OMP) pharmaceuticals: ciprofloxacin (CIP), diclofenac (DCF), and salicylic acid (SA), under different radiation sources: ultraviolet A (UVA), solar light simulator (SLS), blue visible light (BVL) and cold visible light (CVL). CIP and SA were removed effectively via the synergy of adsorption and photocatalysis, while DCF degradation was achieved solely by photocatalysis. After implementing scavenger agents, photocatalytic degradation processes mainly depended on the specific pollutant type, while irradiation sources barely defined the photocatalytic mechanism. On the other hand, changes in irradiation intensity significantly influenced the photolysis process, while photocatalysis was slightly affected, indicating that irradiation spectra are more relevant than intensity.
The Role of Structural Defects in the Growth of Two-Dimensional Diamond from Graphene
Authors: Varlamova Liubov A. , Erohin Sergey V. , Sorokin Pavel B.
Published: November 2022

The presented work is devoted to the study of the formation of the thinnest diamond film (diamane). We investigate the initial stages of diamond nucleation in imperfect bilayer graphene exposed by the deposition of H atoms (chemically induced phase transition). We show that defects serve as nucleation centers, their hydrogenation is energy favorable and depends on the defect type. Hydrogenation of vacancies facilitates the binding of graphene layers, but the impact wanes already at the second coordination sphere. Defects influence of 5|7 is lower but promotes diamondization. The grain boundary role is similar but can lead to the final formation of a diamond film consisting of chemically connected grains with different surfaces. Interestingly, even hexagonal and cubic two-dimensional diamonds can coexist together in the same film, which suggests the possibility of obtaining a new two-dimensional polycrystal unexplored before.
Optimization Temperature Programming of Microwave-Assisted Synthesis ZnO Nanoneedle Arrays for Optical and Surface-Enhanced Raman Scattering Applications
Authors: Chang Tung-Hao , Chang Yu-Cheng , Lee Chung-I , Lin Ying-Ru , Ko Fu-Hsiang
Published: November 2022

This study used a rapid and simple microwave-assisted synthesis method to grow ZnO nanoneedle arrays on the silicon substrate with the ZnO seed layer. The effects of reaction temperature and time on the lengths of ZnO nanoneedle arrays were investigated. The appropriate temperature programming step can grow the longer ZnO nanoneedle arrays at the same reaction time (25 min), which is 2.08 times higher than without the temperature programming step. The geometry of the ZnO nanoneedle arrays features a gradual decrease from the Si substrate to the surface, which provides an excellent progressive refractive index between Si and air, resulting in excellent antireflection properties over an extensive wavelength range. In addition, the ZnO nanoneedle arrays exhibit a suitable structure for uniform deposition of Ag nanoparticles, which can provide three-dimensional hot spots and surface active sites, resulting in higher surface-enhanced Raman scattering (SERS) enhancement, high uniformity, high reusability, and low detection limit for R6G molecule. The ZnO/Ag nanoneedle arrays can also reveal a superior SERS-active substrate detecting amoxicillin (10−8 M). These results are promising for applying the SERS technique for rapid low-concentration determination in different fields.
Facile Synthesis and Characterizations of Mixed Metal Oxide Nanoparticles for the Efficient Photocatalytic Degradation of Rhodamine B and Congo Red Dyes
Authors: Abdelrahman Ehab A. , Al-Farraj Eida S.
Published: November 2022

Photocatalytic degradation has been suggested to be a cheap and efficient way to dispose of organic pollutants, such as dyes. Therefore, our research team strives to produce nanophotocatalysts in a simple and inexpensive way. In this work, the Pechini sol–gel technique was employed for the facile synthesis of Mn0.5Zn0.5Fe2O4/Fe2O3 and Fe0.5Mn0.5Co2O4/Fe2O3 as mixed metal oxide nanoparticles for the efficient photocatalytic degradation of Rhodamine B and Congo Red dyes. XRD, FT-IR, a N2 adsorption/desorption analyzer, EDS, FE-SEM, and an UV–Vis diffuse reflectance spectrophotometer were used to characterize the produced samples. The XRD patterns revealed that the average crystallite size of the Fe0.5Mn0.5Co2O4/Fe2O3 and Mn0.5Zn0.5Fe2O4/Fe2O3 samples is 90.25 and 80.62 nm, respectively. The FE-SEM images revealed that the Fe0.5Mn0.5Co2O4/Fe2O3 sample consists of cubic and irregular shapes with an average diameter of 1.71 µm. Additionally, the Mn0.5Zn0.5Fe2O4/Fe2O3 sample consists of spherical shapes with an average diameter of 0.26 µm. The energy gaps of the Fe0.5Mn0.5Co2O4/Fe2O3 and Mn0.5Zn0.5Fe2O4/Fe2O3 samples are 3.50 and 4.3 eV and 3.52 and 4.20 eV, respectively. In the presence of hydrogen peroxide, the complete degradation of 100 mL of 20 mg/L of Rhodamine B and Congo Red dyes occurred at pH = 8 and 3, respectively, within 50 min, using 0.1 g of the synthesized samples.
Effects of Native Whey Protein and Carbohydrate Supplement on Physical Performance and Plasma Markers of Muscle Damage and Inflammation during a Simulated Rugby Sevens Tournament: A Double-Blind, Randomized, Placebo-Controlled, Crossover Study
Authors: Fabre Marina , Mathieu Bertrand , Tiollier Eve , Leduc Cédric , Clauss Matthieu , Marchand Alexandre , Robineau Julien , Piscione Julien , Serenari Tanguy , Brasy Jacqueline , Guerville Mathilde , Ligneul Amandine , Bigard Xavier
Published: November 2022

The importance of optimized recovery during a sport competition is undisputed. The objective of this study was to determine the effects of recovery drinks comprising either carbohydrate only, or a mix of native whey proteins and carbohydrate to maintain physical performance and minimize muscle damage during a simulated rugby sevens (rugby 7s) tournament. Twelve well-trained male rugby players participated in three simulated rugby 7s tournament days with a week’s interval in between. Each tournament comprised a sequence of three simulated matches, interspersed with 2 h of recovery. Three different recovery drinks were tested: a placebo (PLA, nonenergetic chocolate-flavored drink), a carbohydrate drink (CHO, 80 g of carbohydrate) or an isoenergetic carbohydrate–protein drink (P-CHO, 20 g of Pronativ®, native whey protein and 60 g of carbohydrate). A different recovery drink, consumed after each match, was tested during each simulated tournament. Physical performance, muscle damage and muscle pain were assessed before and after each simulated tournament. Regarding physical performance, both P-CHO and CHO drinks had a positive effect on the maintenance of 50 m sprint time compared to the PLA drink (effect sizes large and moderate, respectively). Regarding muscle damage, the P-CHO supplement attenuated the creatine phosphokinase increase at POST6 compared to PLA (effect size, moderate). Finally, P-CHO and CHO drinks reduced the exercise-induced DOMS (effect size, moderate), compared to the PLA condition (effect size, large), while P-CHO only reduced pain on muscle palpation and pain when descending stairs compared to PLA 24 h post-tournament (effect size, small). This study suggests that consuming a recovery drink containing native whey proteins and carbohydrate or carbohydrate only after each match of a rugby 7s tournament may attenuate the exercise-induced increase in markers of muscle damage and maintain physical performance.
Validation of the Children’s Eating Behaviour Questionnaire in Poland
Authors: Malczyk Żaneta , Kuczka Oliwia , Pasztak-Opiłka Agnieszka , Zachurzok Agnieszka
Published: November 2022

Introduction: Obesity is increasingly diagnosed in pre-school and early primary school children. Eating styles displayed by the youngest children may contribute to the development of overweight and obesity. Their assessment may be extremely important in diagnosing the causes of obesity, but also in planning treatment. Aim of the study: In view of the need to introduce a tool for assessing eating behaviours in children in Poland, the aim of the study was to develop the Polish adaptation of the Children’s Eating Behaviour Questionnaire (CEBQ). Material and methods: The study group consisted of 151 mothers of children aged 3–10 years (M = 6.77, SD = 2.34), who completed the Polish version of the CEBQ. In order to assess the validity of the questionnaire, a factor analysis was conducted, using the principal components method with the Oblimin rotation and Kaiser normalization. To assess the reliability of the questionnaire, its internal consistency was checked by calculating Cronbach’s alpha consistency coefficient. The external validity of the CEBQ was also checked by correlating its scales with those of the Temperament Questionnaire (EAS-C). Results: The principal components analysis extracted an eight-factor scale from the 35 items of the questionnaire in which a total of 60.57% of the common variance was explained. The validity of such an eight-factor solution was confirmed by the Kaiser method. Satisfactorily high values of Cronbach’s alpha internal consistency coefficient were obtained (0.78). Positive correlations were found between emotionality and emotional undereating and overeating, between shyness and fussiness and negative correlations between activity and slowness in eating, sociability and fussiness and slowness in eating and between shyness and enjoyment of food. Conclusions: The Polish version of the CEBQ is characterized by the acceptable validity and reliability and has a satisfactory criterion accuracy; therefore, it can be used as a psychometric tool to assess eating behaviours in Polish children.
Genetic Risk Factors and Gene–Lifestyle Interactions in Gestational Diabetes
Authors: Jääskeläinen Tiina , Klemetti Miira M.
Published: November 2022

Paralleling the increasing trends of maternal obesity, gestational diabetes (GDM) has become a global health challenge with significant public health repercussions. In addition to short-term adverse outcomes, such as hypertensive pregnancy disorders and fetal macrosomia, in the long term, GDM results in excess cardiometabolic morbidity in both the mother and child. Recent data suggest that women with GDM are characterized by notable phenotypic and genotypic heterogeneity and that frequencies of adverse obstetric and perinatal outcomes are different between physiologic GDM subtypes. However, as of yet, GDM treatment protocols do not differentiate between these subtypes. Mapping the genetic architecture of GDM, as well as accurate phenotypic and genotypic definitions of GDM, could potentially help in the individualization of GDM treatment and assessment of long-term prognoses. In this narrative review, we outline recent studies exploring genetic risk factors of GDM and later type 2 diabetes (T2D) in women with prior GDM. Further, we discuss the current evidence on gene–lifestyle interactions in the development of these diseases. In addition, we point out specific research gaps that still need to be addressed to better understand the complex genetic and metabolic crosstalk within the mother–placenta–fetus triad that contributes to hyperglycemia in pregnancy.
Avicularin Attenuates Lead-Induced Impairment of Hepatic Glucose Metabolism by Inhibiting the ER Stress-Mediated Inflammatory Pathway
Authors: Qiu Ting , Shi Jia-Xue , Cheng Chao , Jiang Hong , Ruan Hai-Nan , Li Jun , Liu Chan-Min
Published: November 2022

Lead (Pb), an environmental hazard, causes several human diseases. Avicularin (Avi), a main dietary flavonoid found in several plants and fruits, exhibits potential protective properties on organs. However, the molecular mechanisms of Avi’s protective effects against Pb-induced damage are not clear. In our study, the effects of Avi on Pb-induced hepatotoxicity were evaluated using ICR mice. We have revealed for the first time that treatment with Avi significantly reduced hepatic inflammation, endoplasmic reticulum stress (ERS) and glucose metabolism disorder induced by Pb. Avi decreased the serum biochemical indicators of glucose metabolism. Avi increased the activities of glycogenolysis rate-limiting enzyme hexokinase (HK), pyruvate kinase (PK), glucokinase (GK) and glycogen phosphorylase (PYG) and inhibited the activities of gluconeogenesis rate-limiting enzyme phosphoenolpyruvate carboxy kinase (PEPCK) and glucose-6-phosphate dehydrogenase (G6PD). Avi decreased the protein expression levels of glucose-regulated protein 78 (GRP78), phosphorylated inositol requiring enzyme 1 (p-IRE1), phosphorylated RNA-dependent protein kinase-like ER kinase (p-PERK) and phosphorylated eukaryotic initiation factor 2α (p-eIF2α). The levels of tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) were decreased in the liver as a result of Avi suppression Pb-induced inflammation. These results indicated that Avi attenuated Pb-induced impairment of hepatic glucose metabolism by the ERS and inflammation pathway.
Arctium lappa Lam. and Its Related Lignans Improve Hyperglycemia and Dyslipidemia in Diabetic Rodent Models: A Systematic Review and Meta-Analysis
Authors: Watanabe Shihori , Yamabe Shizuko , Shimada Masako
Published: November 2022

Research on nutraceuticals has focused on reducing the onset, progression, and significant consequences of diabetes mellitus. Arctium lappa Lam. is a great source of plant fibers and polyphenols that have anti-disease benefits, including those for diabetes mellitus. This study sought to determine the impact of Arctium lappa Lam. extracts and its associated lignans on diabetic hyperglycemia and dyslipidemia by conducting meta-analyses of the available research using diabetic rodents. English-language peer-reviewed articles were searched by PubMed and Embase up until 10 August 2022. Included were studies comparing the blood glucose and/or lipid levels of diabetic rodents given either extracts of Arctium lappa Lam. and its related lignans or vehicles. Blood glucose levels were reported in 16 studies involving 168 diabetic mice or rats treated with Arctium lappa Lam. and 168 diabetic controls. The pooled effect size was −1.42 [95% CI: −1.84 to −1.00] with significant heterogeneity. Type of diabetic model was found to be the prominent covariate that explained, at least partially, the heterogeneity. Moreover, diabetic rodents treated with Arctium lappa Lam. showed a notable improvement in their hypertriglyceridemia and hypercholesterolemia.
Prevalence and Implications of Shiga Toxin-Producing E. coli in Farm and Wild Ruminants
Authors: Ray Ritesh , Singh Pallavi
Published: November 2022

Shiga-toxin-producing Escherichia coli (STEC) is a food-borne pathogen that causes human gastrointestinal infections across the globe, leading to kidney failure or even death in severe cases. E. coli are commensal members of humans and animals’ (cattle, bison, and pigs) guts, however, may acquire Shiga-toxin-encoded phages. This acquisition or colonization by STEC may lead to dysbiosis in the intestinal microbial community of the host. Wildlife and livestock animals can be asymptomatically colonized by STEC, leading to pathogen shedding and transmission. Furthermore, there has been a steady uptick in new STEC variants representing various serotypes. These, along with hybrids of other pathogenic E. coli (UPEC and ExPEC), are of serious concern, especially when they possess enhanced antimicrobial resistance, biofilm formation, etc. Recent studies have reported these in the livestock and food industry with minimal focus on wildlife. Disturbed natural habitats and changing climates are increasingly creating wildlife reservoirs of these pathogens, leading to a rise in zoonotic infections. Therefore, this review comprehensively surveyed studies on STEC prevalence in livestock and wildlife hosts. We further present important microbial and environmental factors contributing to STEC spread as well as infections. Finally, we delve into potential strategies for limiting STEC shedding and transmission.
Molecular Characteristics and Antibiotic Resistance of Staphylococcus aureus Isolated from Patient and Food Samples in Shijiazhuang, China
Authors: Zhang Han , Qin Liyun , Jin Caiping , Ju Huidong , Jiang Ruiping , Li Lijie , Zhang Hong , Gao Weili , Wei Xiuping , Dong Hongyan , Lu Fei , Lv Guoping
Published: November 2022

Staphylococcus aureus (S. aureus) is a common opportunistic and zoonotic pathogen in the world and could easily cause human infections and food contaminations. This study investigated the sequence typing and resistance profiles of S. aureus isolates from patient and food samples in Shijiazhuang, China. A total of 101 S. aureus isolates were distributed into six clonal complexes (CCs) and 16 singletons. A total of 86 patient isolates were distributed into six clonal CCs and 12 singletons, including a new ST. CC59, CC5, CC22, and CC398 were the predominant CCs of patient isolates. A total of 15 foodborne S. aureus isolates were distributed into 3 CCs and 4 STs, and CC1 was the most prevalent CC. Moreover, 101 S. aureus isolates had high resistance to penicillin and low resistance to chloramphenicol and rifampicin. A total of 39 strains of methicillin-resistant Staphylococcus aureus (MRSA) were detected in this study, including thirty-eight strains of patient isolates (44.2%, 38/86) and one strain of food isolates (6.7%, 1/15). MRSA-ST5, MRSA-ST59, and MRSA-ST239 were the predominant MRSA isolates in hospitals. The present study explained the relationship between S. aureus isolated from patient and food samples and indicated the risks of S. aureus in infectious diseases.
Long-Term Pulmonary Dysfunction by Hyperoxia Exposure during Severe Viral Lower Respiratory Tract Infection in Mice
Authors: Lilien Thijs A. , Gunjak Miša , Myti Despoina , Casado Francisco , van Woensel Job B. M. , Morty Rory E. , Bem Reinout A.
Published: November 2022

Viral-induced lower respiratory tract infection (LRTI), mainly by respiratory syncytial virus (RSV), causes a major health burden among young children and has been associated with long-term respiratory dysfunction. Children with severe viral LRTI are frequently treated with oxygen therapy, hypothetically posing an additional risk factor for pulmonary sequelae. The main goal of this study was to determine the effect of concurrent hyperoxia exposure during the acute phase of viral LRTI on long-term pulmonary outcome. As an experimental model for severe RSV LRTI in infants, C57Bl/6J mice received an intranasal inoculation with the pneumonia virus of mice J3666 strain at post-natal day 7, and were subsequently exposed to hyperoxia (85% O2) or normoxia (21% O2) from post-natal day 10 to 17 during the acute phase of disease. Long-term outcomes, including lung function and structural development, were assessed 3 weeks post-inoculation at post-natal day 28. Compared to normoxic conditions, hyperoxia exposure in PVM-inoculated mice induced a transient growth arrest without subsequent catchup growth, as well as a long-term increase in airway resistance. This hyperoxia-induced pulmonary dysfunction was not associated with developmental changes to the airway or lung structure. These findings suggest that hyperoxia exposure during viral LRTI at young age may aggravate subsequent long-term pulmonary sequelae. Further research is needed to investigate the specific mechanisms underlying this alteration to pulmonary function.
Intrauterine Transmission of Hepatitis C Virus Concomitant with Isolated Severe Fetal Ascites
Authors: Rădoi Cristiana Luiza , Berbecaru Elena-Iuliana-Anamaria , Istrate-Ofițeru Anca-Maria , Nagy Rodica Daniela , Drăgușin Roxana Cristina , Căpitănescu Razvan Grigoraș , Zorilă Marian Valentin , Zorilă Lucian George , Iliescu Dominic Gabriel
Published: November 2022

Background: Perinatal Hepatitis C Virus (HCV) transmission occurs in 4–7% of the cases with detectable viremia at delivery. HCV testing in pregnancy is recommended. The fetal infection was previously described as asymptomatic although there are two cases, including this one, to report the presence of isolated fetal ascites in HCV infected fetuses. Case report: A 42-year-old patient, 3G, 3P, presented in the Emergency Room for painful uterine contraction. The third-trimester ultrasound examination noted severe fetal ascites, accompanied by hyperechoic bowels and polyhydramnios. The diagnosis required a detailed ultrasound exam, invasive testing (amniocentesis, cordocentesis, and fetal paracentesis), and a complete workup. The mother tested positive for HCV antibodies, and the fetal cord blood tested positive for HCV RNA. The ascites resolved after paracentesis, and the gastrointestinal and respiratory functions markedly improved. The fetus was delivered at term in good condition. Conclusions: The etiology of isolated fetal ascites is broad. This case may indicate that intrauterine HCV transmission is a potential cause of isolated fetal ascites in the absence of other explanation, and isolated fetal ascites can be the only sign revealed on a routine examination. We suspected, having no other detected cause for ascites, the intrauterine transmission of HCV. Invasive procedures, such as paracentesis, are required for abdominal decompression to manage isolated fetal ascites, as it may be a saving procedure. A genetic investigation is needed, and a good neonatal outcome is expected in the absence of fetal structural or genetic abnormalities, as in our case.
Design, Synthesis, and Antiprotozoal Evaluation of New Promising 2,9-Bis[(substituted-aminomethyl)]-4,7-phenyl-1,10-phenanthroline Derivatives, a Potential Alternative Scaffold to Drug Efflux
Authors: Guillon Jean , Cohen Anita , Boudot Clotilde , Monic Sarah , Savrimoutou Solène , Moreau Stéphane , Albenque-Rubio Sandra , Lafon-Schmaltz Camille , Dassonville-Klimpt Alexandra , Mergny Jean-Louis , Ronga Luisa , Bernabeu de Maria Mikel , Lamarche Jeremy , Lago Cristina Dal , Largy Eric , Gabelica Valérie , Moukha Serge , Dozolme Pascale , Agnamey Patrice , Azas Nadine , Mullié Catherine , Courtioux Bertrand , Sonnet Pascal
Published: November 2022

A series of novel 2,9-bis[(substituted-aminomethyl)]-4,7-phenyl-1,10-phenanthroline derivatives was designed, synthesized, and evaluated in vitro against three protozoan parasites (Plasmodium falciparum, Leishmania donovani and Trypanosoma brucei brucei). Pharmacological results showed antiprotozoal activity with IC50 values in the sub and μM range. In addition, the in vitro cytotoxicity of these original molecules was assessed with human HepG2 cells. The substituted diphenylphenanthroline 1l was identified as the most potent antimalarial derivative with a ratio of cytotoxic to antiparasitic activities of 505.7 against the P. falciparum CQ-resistant strain W2. Against the promastigote forms of L. donovani, the phenanthrolines 1h, 1j, 1n and 1o were the most active with IC50 from 2.52 to 4.50 μM. The phenanthroline derivative 1o was also identified as the most potent trypanosomal candidate with a selectivity index (SI) of 91 on T. brucei brucei strain. FRET melting and native mass spectrometry experiments evidenced that the nitrogen heterocyclic derivatives bind the telomeric G-quadruplexes of P. falciparum and Trypanosoma. Moreover, as the telomeres of the parasites P. falciparum and Trypanosoma could be considered to be possible targets of this kind of nitrogen heterocyclic derivatives, their potential ability to stabilize the parasitic telomeric G-quadruplexes have been determined through the FRET melting assay and by native mass spectrometry.
Human Umbilical Cord MSC Delivered-Soluble TRAIL Inhibits the Proliferation and Promotes Apoptosis of B-ALL Cell In Vitro and In Vivo
Authors: Chen Fangshan , Zhong Xianmei , Dai Qian , Li Kuo , Zhang Wei , Wang Jie , Zhao Yueshui , Shen Jing , Xiao Zhangang , Xing Hongyun , Li Jing
Published: November 2022

The TNF-related apoptosis-inducing ligand (TRAIL) could induce apoptosis of leukemic cells, while showed no cytotoxic effect on normal cells. One of the limitations for application of recombinant TRAIL (rhTRAIL) in leukemia treatment is that the serum half-life of this protein is short. Gene delivery is a good strategy to prolong the half-life of TRAIL. In this study, we genetically engineered umbilical cord-MSCs to continuously express and secrete soluble TRAIL (MSC-sTRAIL), to investigate the effects of MSC-sTRAIL on B-cell acute lymphocytic leukemia (B-ALL) cells. In vitro, MSC-sTRAIL significantly inhibited the proliferation of B-ALL cells by suppressing PI3K/AKT and MEK/ERK signaling pathways, and induced apoptosis of B-ALL cells via the caspase cascade-mediated pathway and mitochondrial-mediated pathway. In vivo, MSC-sTRAIL dramatically inhibited B-ALL cell growth. Meanwhile, B-ALL-induced splenic and renal injuries were significantly alleviated after MSC-sTRAIL treatment. Moreover, the serum levels of MSC-secreted sTRAIL were still high in MSC-sTRAIL treated mice, indicating an extended half-life of sTRAIL. Our study suggests that MSC delivered-TRAIL secretion is a potential therapeutic strategy for B-ALL treatment.
Three-Dimensional Microtumor Formation of Infantile Hemangioma-Derived Endothelial Cells for Mechanistic Exploration and Drug Screening
Authors: Li Yanan , Zhu Xinglong , Kong Meng , Chen Siyuan , Bao Ji , Ji Yi
Published: November 2022

Infantile hemangioma (IH) is the most prevalent type of vascular tumor in infants. The pathophysiology of IH is unknown. The tissue structure and physiology of two-dimensional cell cultures differ greatly from those in vivo, and spontaneous regression often occurs during tumor formation in nude mice and has severely limited research into the pathogenesis and development of IH. By decellularizing porcine aorta, we attempted to obtain vascular-specific extracellular matrix as the bioink for fabricating micropattern arrays of varying diameters via microcontact printing. We then constructed IH-derived CD31+ hemangioma endothelial cell three-dimensional microtumor models. The vascular-specific and decellularized extracellular matrix was suitable for the growth of infantile hemangioma-derived endothelial cells. The KEGG signaling pathway analysis revealed enrichment primarily in stem cell pluripotency, RAS, and PI3KAkt compared to the two-dimensional cell model according to RNA sequencing. Propranolol, the first-line medication for IH, was also used to test the model’s applicability. We also found that metformin had some impact on the condition. The three-dimensional microtumor models of CD31+ hemangioma endothelial cells were more robust and efficient experimental models for IH mechanistic exploration and drug screening.
Mutual Jellification of Two Bactericidal Cationic Polymers: Synthesis and Physicochemical Characterization of a New Two-Component Hydrogel
Authors: Alfei Silvana , Zorzoli Alessia , Marimpietri Danilo , Schito Anna Maria , Russo Eleonora
Published: November 2022

Here, a new two-component hydrogel (CP1OP2-Hgel) was developed, simply by dispersing in water two cationic bactericidal polymers (CP1 and OP2) effective against several multidrug-resistant (MDR) clinical isolates of the most relevant Gram-positive and Gram-negative species. Interestingly, while OP2 acts only as an antibacterial ingredient when in gel, CP1 works as both an antibacterial and a gelling agent. To verify whether it would be worthwhile to use CP1 and OP2 as bioactive ingredients of a new hydrogel supposed for a future treatment of skin infections, dose-dependent cytotoxicity studies with CP1 and OP2 were performed on human fibroblasts for 24 h, before preparing the formulation. Although a significant cytotoxicity at concentrations > 2 µM was evidenced for both polymers, selectivity indices (SIs) over 12 (CP1) and up to six (OP2) were determined, due to the powerful antibacterial properties of the two polymers, thus supporting the rationale for their formulation as a hydrogel. The chemical structure and morphology of CP1OP2-Hgel were investigated by PCA-assisted attenuated total reflectance (ATR) Fourier-transform infrared (FTIR) analysis and scanning electron microscopy (SEM), while its rheological properties were assessed by determining its dynamic viscosity. The cumulative weight loss and swelling percentage curves, the porosity, and the maximum swelling capability of CP1OP2-Hgel were also determined and reported. Overall, due to the potent bactericidal effects of CP1 and OP2 and their favorable selectivity indices against several MDR pathogens, good rheological properties, high porosity, and strong swelling capability, CP1OP2-Hgel may, in the future, become a new weapon for treating severe nosocomial skin infections or infected chronic wounds. Further investigations in this sense are currently being carried out.
Ultrahigh Q-Guided Resonance Sensor Empowered by Near Merging Bound States in the Continuum
Authors: Liu Zhiran , Zhou Yi , Guo Zhihe , Zhao Xuyang , Luo Man , Li Yuxiang , Wu Xiang
Published: November 2022

Bound states in the continuum (BICs) have attracted a lot of interest in the field of nanophotonics, and provide an important physical mechanism to realize high quality (Q) factor resonance. However, in practice, manufacturing error will greatly affect the Q factor. In this paper, we propose an asymmetric metasurface supporting near merging BIC under normal incidence. Such near merging BIC can achieve a higher Q factor (>107) than common structures (Q ~ 105) with the same degree of asymmetry in the structure. Moreover, the near merging BICs also show higher surface sensitivity than other resonant modes. Our work provides a promising approach for the realization of a high-performance biosensing platform.
Reconstructed Hybrid Optical OFDM-NOMA for Multiuser VLC Systems
Authors: Li Baolong , Shi Jianfeng , Feng Simeng
Published: November 2022

Non-orthogonal multiple access (NOMA) is deemed to be a prospective multiple access technology of the next generation. However, in visible light communication (VLC), when advanced hybrid optical orthogonal frequency division multiplexing (O-OFDM), such as hybrid asymmetrically clipped O-OFDM (HACO-OFDM), is combined with NOMA, error propagation is induced, which degrades the system performance. Therefore, a novel reconstructed hybrid O-OFDM-NOMA (RHO-OFDM-NOMA) scheme is conceived in this paper. In order to eliminate the error propagation, the users in RHO-OFDM-NOMA opt for the ACO-OFDM or clipping-free O-OFDM signals according to their channel qualities, which are subsequently superimposed on pulse-amplitude-modulated discrete multitone (PAM-DMT) to yield the spectrum-efficient hybrid O-OFDM signal. Furthermore, a reconstruction process is designed to ensure the non-negativity. Compared with HACO-OFDM, the proposed RHO-OFDM can retain the error propagation in NOMA-VLC, whilst maintaining the superiorities of high spectral and power efficiency. It is demonstrated by simulation results that RHO-OFDM-NOMA can support a notably higher data rate than the NOMA schemes using conventional O-OFDM.
What Can We Learn from Entanglement and Quantum Tomography?
Authors: Ralston John P.
Published: November 2022

Entanglement has become a hot topic in nuclear and particle physics, although many physicists are not sure they know what it means. We maintain that an era of understanding and using quantum mechanics on a dramatically new basis has arrived. We review a viewpoint that treats the subject as being primarily descriptive and completely free of the intellectual straitjackets and mysticism argued over long ago. Quantum probability is an extension of classical probability, but with universal uses. Density matrices describe systems where entanglement or its absence is a classification tool. Most of these have been known for decades, but there is a new way of understanding them that is liberated from the narrow outlook of the early days.
HPLC-DAD-MS Identification and Quantification of Phenolic Components in Japanese Knotweed and American Pokeweed Extracts and Their Phytotoxic Effect on Seed Germination
Authors: Mikulic-Petkovsek Maja , Veberic Robert , Hudina Metka , Misic Eva
Published: November 2022

We performed a detailed HPLC-MSn analysis of the phenolic compounds from the extracts of two invasive alien plant species (IAPS): Japanese knotweed (Fallopia japonica (Houtt.) Ronse Decr.) and American pokeweed (Phytolacca americana L.). The major phenolic groups were hydroxycinnamic acids and flavanols in Japanese knotweed (J. knotweed) and flavonols, hydroxycinnamic acids, and stilbenes in American pokeweed (A. pokeweed). We investigated the influence of solvent type and extraction time on the extraction efficiency of the phenolic compounds. The solvent 80% methanol had a higher polyphenolic extraction efficiency than water, since 14.5 times more flavonols and 2.3 times more stilbenes were extracted from J. knotweed and 5.2 times more flavonols and 2.6 times more stilbenes were extracted from A. pokeweed. In contrast, with water, we obtained a 52% higher hydroxycinnamic acids (HCA) content from J. knotweed. Hydroxycinnamic acids were best extracted in water after 24 h, flavanols after 12 h, stilbenes between 12 and 24 h, and flavonol glycosides after 48 h of extraction. We also tested the allelopathic effect of the aqueous extract of A. pokeweed and J. knotweed on seed germination and shoot and root growth of perennial ryegrass. The results showed that the water extract of J. knotweed resulted in 38 to 48% lower seed germination of perennial ryegrass, and the extract of A. pokeweed resulted in 83 to 90% lower seed germination. The phytotoxic effect of the extract of J. knotweed and A. pokeweed was also reflected in a characteristic reduced growth of shoots and roots of perennial ryegrass. The phytotoxic action of IAPS could also be applied for beneficial purposes, since this would be an effective strategy for their control and a reduction of their spread in the environment.
Non-Authenticity of Spring Barley Genotypes Revealed in Gene Bank Accessions
Authors: Dreiseitl Antonín , Zavřelová Marta
Published: November 2022

Plant research and breeding depends on plant genotypes; therefore, genotype authenticity of accessions is the basic requirement for users of gene banks. Surprisingly, this extremely important topic is rarely reported in the scientific community. Non-authentic are accessions that are mislabelled and undesirable genotypes of heterogeneous accessions. In barley, we try to uncover both named problems on the basis of postulated major powdery mildew resistance genes. These are diverse, environmentally stable and their use is well documented and suitable for genotype characterization. In this contribution, we postulate resistance genes in 15 varieties represented by 157 derived lines of 32 accessions originating from seven foreign gene banks and compare these findings with previous results including those 15 identically labelled varieties from our domestic gene bank. We found that 37.5% of the gene bank accessions investigated herein were heterogeneous, and at least 20.0% were mislabelled. A large-scale molecular characterisation of varieties is now being carried out, and using authentic varieties must be one of the key requirements. Therefore, accessions of each variety from a minimum of three gene banks whose identity has been verified by reliable methods should be compared before starting new experiments. These will involve molecular varietal characterisation to serve as a foundation for future plant science research and effective crop improvement.