bluuebunny/crossref_metadata_2025 huggingface dataset:
Ekim Öncesi Bazı Uygulamaların Kereviz Tohumlarının Fide Performansına Etkileri
Authors: DEMİRKES Merve , DUMAN İbrahim
Published: December 2021

Kereviz tohumlarında kademeli tohum olgunluğu ve tohumdan kaynaklı faktörlerin etkisi ile çimlenme ile fide çıkışı geç ve düzensiz gerçekleşmektedir. Bu sorun ön koşullandırma (priming) uygulamaları ile giderilebilmektedir. Buradan hareketle kereviz tohumlarına ekim öncesinde yapılan bazı uygulamaların kereviz fidesi üretimindeki çimlenme performansına olan etkilerinin belirlenmesi amacıyla planlanan çalışma 2021 yılında kontrollü laboratuvar koşullarında yürütülmüştür. Çanakkale kereviz çeşidinin tohumlarının kullanıldığı çalışmada, çimlenmenin teşvik edilmesi amacıyla ekim öncesi potasyum tuzlarından KH₂PO₄’ın iki farklı dozunda (0.5 mol ve 1 mol) priming uygulaması, ön üşütme (5 gün ve 7 gün) ve humudifikasyon (36 saat ve 48 saat) uygulamalarından yararlanılmıştır. Uygulamalardan sonra optimum nem içeriğine kadar kurutulan kereviz tohumları kontrol tohumları ile birlikte ISTA (2014) kuralları çerçevesinde çimlendirme testine alınmıştır. Çimlenme oranı (%) ve ortalama çimlenme zamanı (gün) değerlerinin irdelendiği çalışmada, çimlenme oranı (%) bakımından uygulamalar arasında istatistiki anlamda önemli bir farklılık belirlenmemiştir. Buna karşılık ortalama çimlenme zamanı (gün) bakımından uygulamalar arasındaki fark p ≤ 0.001 güvenle önemli bulunmuştur. Kontrol tohumlarının 8.1 günde ulaştığı ortalama çimlenme zamanına KH₂PO₄ uygulamasında 6.0 günde, ön üşütme uygulamasında 7.4 günde ulaşılmıştır. Uygulama dozlarının etkisi de p ≤ 0.001 güvenle önemli bulunmuştur. Yedi gün gerçekleştirilen ön üşütme uygulaması ile en yüksek çimlenme oranı değerine (%92.75) ulaşılırken ortalama çimlenme zamanı değerleri ise kontrol tohumlarında 7.9-8.3 gün, 0.5 ve 1 mol KH₂PO₄ uygulamalarında ise 5.7 ve 6.3 gün tespit edilmiştir.
Kavun Tohumu Ekstraksiyonunda Hidroklorik Asit Kullanımının Tohum ve Fide Özellikleri Üzerine Etkisi
Authors: ÖZTÜRK Alperen , İKİZ Boran , ARPACI Bekir Bülent , DAŞGAN Hayriye
Published: December 2021

Kavun tohumu çıkarılırken meyve eti, plasenta ve tohum etrafındaki jelimsi tabakadan arındırılması gerekmektedir. Bu işlem için yaygın olarak kullanılan fermantasyon yöntemine alternatif olarak bazı kimyasalların kullanılması zaman ve işgücü tasarrufu sağlamaktadır. Bu çalışmada hidroklorik asit uygulamasının kavun tohumlarındaki ekstraksiyon işlemini kolaylaştırıcı, zaman kazandırıcı etkileri ile çimlenme, çıkış ve fide büyümesi üzerine etkileri incelenmiştir. Bitkisel materyal olarak Sena F1 kavun çeşidine ait tohumlar kullanılmıştır. Tohumlar çekirdek evinden çıkarılarak etraflarındaki jelimsi madde, bir miktar meyve eti ve plasenta ile beraber %1, 5, 10, 15, 20 konsantrasyonlarındaki hidroklorik asit (HCl) çözeltileri içerisinde 5, 10, 20, 30 dakika süreyle bekletilmiştir. Uygulamaların, tohum çimlenme oranı ve hızı, çıkış oranı ve hızı üzerine etkileri ile fidelerde bitki boyu, yeşil aksam yaş ağırlığı ve hipokotil boyu üzerine etkileri değerlendirilmiştir. Bu çalışmayla kavunda yüksek dozda hidroklorik asit ile ekstraksiyonda doz ve bekletme süresi belirlenmiştir. Çalışmanın sonuçlarına göre HCl kullanımında tohumların kolay ayrıldığı bu bağlamda klasik fermantasyon yöntemine göre zamandan ve işgücünden kazanç sağlandığı görülmüştür. Kavun tohumlarının hidroklorik asit ile ekstraksiyonunda, yüksek tohum çıkış oranı ve yeterli fide gelişimi dikkate alındığında düşük asit konsantrasyonunda %1 HCl - 20 dakika veya kısa bekleme süresi tercih edilirse %5 HCl-5 dakika uygulamalarının önerilebileceği belirlenmiştir.
Türkiye Tohumculuğunun Tarihsel Gelişimi, Mevcut Durumu, Problemleri ve Çözüm Önerileri
Authors: BAĞCI Seydi Ahmet , ÖZER İrfan
Published: December 2021

Ülkemizde planlı bitki ıslahı çalışmaları Cumhuriyet’in ilanından sonra-1925 yılından itibaren- tohum
ıslah istasyonlarının kurulması ile başlamıştır. 1928 yılında Ankara’da kurulan Umum Ziraat Laboratuvarı, 1930
yılında Tohum Islah İstasyonuna, 1936 yılında da deneme yapma yetkisi de verilerek Tohum Islah ve Deneme
İstasyonuna dönüştürülmüştür. 1963 yılında "308 sayılı Tohumlukların Tescil, Kontrol ve Sertifikasyonu
Hakkında Kanun’un çıkarılmasıyla ülkemiz tohumluk üretiminde yeni bir dönem başlamıştır. 2004 yılında “Yeni
Bitki Çeşitlerine Ait Islahçı Haklarının Korunmasına İlişkin 5042 sayılı Kanun” ve 2006 yılında “5553 sayılı
Tohumculuk Kanunu” yürürlüğe girmiştir. 2008 yılından itibaren özel sektörün yeniden yapılanması 5553 sayılı
Tohumculuk Kanunu kapsamında büyük ölçüde tamamlanmıştır. Bu gelişmelerin bir sonucu olarak 1996 üretim
sezonunda 110.000 ton, 2002 üretim sezonunda 145.000 ton, 2006 üretim sezonunda 371.000 ton olarak
gerçekleşen sertifikalı tohumluk üretim miktarı ve dağıtımı, 2020 üretim sezonunda 1.241.000 ton üretim
miktarına ulaşmıştır. Kendine döllenen bitkilerin ıslahı ve tohumluk üretimlerinde olumlu gelişmeler yaşanırken,
mısır, ayçiçeği, şeker pancarı ve hibrit sebze alanlarında yerli çeşit ıslah çalışmalarında istenilen düzeyde bir
gelişme sağlanamamıştır. Tohumculuk sektörünün ülkemizde istenen seviyeye gelebilmesi için destekleme ve
denetleme konularında yeni yaklaşımlara ihtiyaç duyulmaktadır.
Arpa (Hordeum vulgare L.)’da Hesse Sineği [(Mayetiola destructor (Say) (Diptera: Cecidomyiidae)]’ne Karşı Tohum İlaçlarının Verim, Morfolojik ve Bazı Agronomik Özelliklerine Etkileri
Authors: KONUKSAL Ayda , GÜLLÜ Mustafa , GÖZÜAÇIK Celalettin , HEKİMHAN Hakan , DEĞİRMENCİ Reşat , KARACA Cem
Published: December 2021

Hesse sineği, Mayetiola destructor (Say) (Diptera: Cecidomyiidae) Kuzey Kıbrıs’ta arpa bitkisinin önemli zararlılarından
biridir. Çalışmada, M. destructor’a karşı bazı sistemik tohum ilaçlarının (İmidacloprid 600 g L-1 (IM), Clothianidin 600 g L-1 (CL) ve
Thiamethoxam 350 g L-1 (TH)) 3 farklı dozu uygulanmıştır. Denemeler, 3 tekerrürlü olarak tesadüf bloklarında şerit parseller deneme
deseninde Athenais çeşidi kullanılarak 2 farklı lokasyonda (Türkmenköy+Paşaköy ve Güzelyurt) 3 üretim sezonu (2013-2016) boyunca
yürütülmüştür. Arpa bitkisinin çıkışından ve 10 cm boya gelmesinden sonra haftada bir defa olmak üzere her parselden tesadüfi olarak
alınan 25 bitkide larva sayımları yapılmıştır. Elde edilen veriler Jmp 13 istatistik paket programında analiz edilmiş ve değerler ilaçsız
kontrol parselleriyle karşılaştırılmıştır. Çalışmaların sonucunda, kontrol parsellerinde ortalama 3.6 birey bitki-1 bulunurken, uygulanan en
yüksek dozla bu değer 0.8 birey bitki-1 bulunmuştur. Her 3 yılın ortalamaları dikkate alındığında kardeş sayısı en yüksek CL
uygulamasından Güzelyurt’ta (3.25 kardeş bitki-1) elde edilmiştir. Hektolitre ağırlığı, Güzelyurt’ta 60.96 kg iken, Paşaköy’de 48.82 kg
olarak gerçekleşmiştir. İlaç uygulamalarında hektolitre; CL’da 55.51 kg, IM’da 54.49 kg, TH’de 54.66 kg olmuştur. Dozlar arasında ise,
en yüksek hektolitre ağırlığı 2. ve 3. dozlarda bulunmuştur. En yüksek bin tane ağırlığı Paşaköy lokasyonunda her iki bitki koruma
ürünün en yüksek dozlarından elde edilmiştir. Hesse sineği için ekim öncesi tohuma ilaç uygulamalarında en yüksek verim, kontrole
(117.4 kg da-1) göre tüm uygulanan ilaçların en yüksek uygulandığı dozlarından elde edilmiştir. Ancak, TH 44.7 kg da-1 (162.1 kg da-1),
IM 38.5 kg da-1 (155.9 kg da-1) ve CL 47.2 kg da-1 (164.6 kg da-1) verim farkı oluşmuştur. Tohum ilaçlamasının yapıldığı parseller ile
ilaçlama yapılmayan parseller karşılaştırıldığında dekara % 37.1 oranında (43.5 kg da-1) verim artışı tespit edilmiştir.
Combined Application of Microbial Inoculation and Biochar to Mitigate Drought Stress in Wheat
Authors: ÇIĞ Fatih , ERMAN Murat , CERİTOĞLU Mustafa
Published: December 2021

Drought stress spearheads the main factors threatening food security. Although many
strategies might use for stress management, microbial inoculation with plant growth promoting bacterias
(PGPBs) which have ACC deaminase activity and biochar amendment which is an effective way to
increase soil carbon stock, improve soil physiological and biological properties are sustainable and easyapplicable methods. The experiment was laid out in a 3x10 factorial design with three replications under
controlled conditions in 2020. The aim of this study was to evaluate the efficiency of microbial
inoculation (MI: TV24C + TV126C + TV61C) and biochar amendments (1%BC, 2%BC and 4%BC)
on growth of wheat seedlings under different irrigation levels (IL1: 80%, IL2: %50 and IL3: 25%).
While biochar applications and microbial inoculation backed up to plants to alleviate drought stress,
most effective results were obtained with combined applications of them. The combined application of
4% biochar+microbial inoculation increased plant height, shoot fresh weight, shoot dry weight, root
fresh weight and root dry weight up to 28.3%, 56.8%, 72.2%, 141.3% and 112.8% compared with
control plants while it improved them up to 4.9%, 10.3%, 16.6%, 21.1% and 40.3% compared with
optimum synthetic fertilizer under drought conditions, respectively. In conclusion, biochar applications
with microbial inoculations can be considered as an effective method to cope with the destructive effects
of drought.
Experimental study of the possibility of reducing the cardiotoxic effects of “Mitomycin C” using an immobilized form of “Mitomycin C” and cytoprotector
Authors: Mikhail P. Gladchenko , Elena B. Artyushkova , Oxana G. Frolova , Galina S. Mal , Vladimir V. Khvostovoy , Anna M. Bykanova , Marina A. Chernyatina , Irina R. Puchak
Published: December 2021

The objective of this work was to investigate in experiments on laboratory rats the possibility of reducing the cardiotoxicity of the anticancer drug Mitomycin C by mixing it with Mesogel before injection and using Mexicor® as a cardioprotective agent. Materials and methods. To carry out the study, 70 male Wistar rats were used. The body weight of laboratory animals at the beginning of the experimental study ranged from 180 to 220 grams. Intraperitoneal administration of Mitomycin C at a dose of 4 mg/kg once at the start of the study was used. When Mitomycin C was administered together with Mesogel, the drugs were combined under aseptic conditions before intraperitoneal administration, and Mesogel was administered at a volume of 10.7 ml/kg. The drug Mexicor® was administered in the form of intramuscular injections at a dose of 60 mg/kg per day for 14 days. Results. After intraperitoneal injection of the drug "Mitomycin C" at a dose of 4 mg / kg, a noticeable decrease in the contractility of the rat myocardium during exercise tests was observed only on the 14th day. The use of "Mitomycin C" mixed with "Mesogel" during intraperitoneal administration had a positive effect, but did not completely exclude the manifestation of the cardiotoxic effects of "Mitomycin C". The greatest efficiency was demonstrated in groups of animals that received "Mitomycin C" in a mixture with "Mesogel" together with the cytoprotective drug "Mexicor®". Conclusion. The combination of "Mitomycin C" immobilisation in "Mesogel" together with the cytoprotective drug "Mexicor®" was found to eliminate the cardiotoxic effects of "Mitomycin C" to the greatest extent.
Role of transforming growth factor beta in tumor process
Authors: Kukes Vladimir G. , Prokofiev Alexey B. , Parfenova Olga K. , Aleksandrova Tatyana V. , Gazdanova Albina A. , Kosenko Valentina V. , Trapkova Alla A.
Published: December 2021

The study and application of representatives of the transforming growth factor-beta (TGF-β) family in practical medicine remains an urgent task at the present time. A more detailed study of the properties and activity of proteins related to TGF-β opens up new possibilities in the treatment and diagnosis of diseases associated with skeletal muscles, female reproductive system, oncology and the cardiovascular system, as well as in the development of drugs based on them. This work examines the role of TGF-β in tumor development and the potential of this protein as a therapeutic target, as well as the signaling pathway, various marker proteins, and various types of receptors involved in this process. During tumor development, TGF-β uses two pathways: the classic SMAD-dependent pathway and the non-SMAD-dependent pathway. In the early stages of tumorigenesis, TGF-β acts as a tumor suppressor, causing a cytostatic effect and apoptosis in normal and pre-malignant cells. However, after tumor development, TGF-β functions as a tumor promoter, triggering the transition of the epithelium to the mesenchyme, which leads to increased invasiveness and the development of metastases. The role of TGF-β in oncogenesis in various organ systems has been studied, including in breast, colon, stomach, hepatocellular carcinoma, thyroid cancer, etc. Specifically, TGF-β1 causes a wide range of different physiological reactions, regulates development, differentiation, carcinogenesis and tumor progression of epithelial cells, has multiple effects on the entire process of hematopoiesis. Directions for the creation of drugs for the treatment of tumors targeting TGF-β are presented.
A coerência segundo a perspectiva da teoria da linguagem de Eugenio Coseriu
Authors: Pinheiro Clemilton Lopes , Santos de Oliveira Jéssica
Published: December 2021

Em trabalhos já historicamente reconhecidos na Linguística Textual, a coerência, de forma geral, tem sido concebida como mensagem ou conteúdo do texto, ou ainda como uma rede de relações conceituais que se estabelecem na tessitura textual. Neste trabalho, a partir da teoria da linguagem de Eugenio Coseriu na qual ele propõe a existência de três níveis da linguagem (universal, histórico e textual), revemos os traços (nem sempre conciliáveis) imputados à coerência. Nosso objetivo é, assim, apresentar uma proposta de compreensão da coerência com base na teoria dos níveis da linguagem. Como conclusão, consideramos que a coerência não é um fenômeno único e homogêneo. Trata-se, ao contrário, de um fenômeno tripartido do qual emergem três objetos teóricos, cada um deles alinhado a um nível da linguagem.
Genotypes and Phenotypes: A Review of Pulmonary Hypertension in Genetic Syndromes
Authors: Sullivan Rachel T , Austin Eric D
Published: December 2021

There has been significant advancement in the understanding of the genetics of pulmonary hypertension (PH), particularly in those with heritable or idiopathic pulmonary arterial hypertension. In addition to genetic variants with a primarily pulmonary vascular disease phenotype, the prevalence of PH in other genetic syndromes is increasingly recognized. We will review the current knowledge of PH associated with multisystem genetic syndromes. There is high prevalence of coexisting cardiac and pulmonary disease, making it challenging to discern whether PH is secondary to these processes or underlying genetic makeup. There is a paucity of data on response to PH-targeted therapy and implications on overall prognosis.
Ekofilozofia a „filozofia zrównoważonego rozwoju”
Authors: Hull Zbigniew
Published: December 2021

Strzeszczenie nie jest dostępne.
Sprawiedliwy rozdział świadczeń medycznych jako problem bioetyczny
Authors: Konstańczak Stefan
Published: December 2021

Streszczenie nie jest dostępne
Antropologiczne implikacje wychowania do zrównoważonego rozwoju
Authors: Niemiec Henryk
Published: December 2021

Streszczenie nie jest dostępne
Üniversite Öğrencilerinde Dışavurumcu Teknikler Çerçevesinde Tasarlanan Yaşam Anlamı Grup Çalışmasının Yaşam Anlamı, Amaç Bilinci ve Depresyon Düzeyine Etkisinin İncelenmesi
Authors: Aydoğdu Bilge Nuran , Eryigit Derya
Published: December 2021

Bu çalışmanın amacı üniversite öğrencilerine yönelik geliştirilen 8 haftalık yaşam anlamı grup çalışmasının etkililiğini test etmektir. Psikolojik iyi oluşun, özellikle danışmanlık sürecinin hümanist teorilerinde vurgulandığı önemli bir yönü, yaşamda algılanan anlamdır. “Amaç” yapısı çeşitli şekillerde tanımlanmış olsa da, tanımlar hayattaki kapsayıcı bir yönün üzerinde uzlaşma eğilimindedir. Hayattaki amaç Frankl tarafından hedeflere sahip olmak ve yaşamın tatmin edici ve değerli olduğuna inanmak olarak tanımlanmıştır. Bireylerin yaşamdaki amaçlarına dair bilinçli oluşu, bir amaç belirlemiş oluşunun bireyin iyi oluşunu destekleyecek bir sistem sunacağı düşünülmektedir. Bu nedenle Marmara Üniversitesi Psikolojik Danışma ve Rehberlik Uygulama Araştırma Merkezi (MARPAM) üzerinden gerçekleştirilen duruyu sonucunda çalışmaya katılmaya gönüllü olan sekiz öğrenci ile grup oturumları düzenlenmiştir. Çalışmada ön test son test deney gruplu yarı deneysel yöntem kullanılmıştır. Oturumlar öncesi katılımcılardan Yaşam Anlamı Ölçeği, Amaç Bilinci (Hissi) Ölçeği ve DAS-21 ölçeği Depresyon alt boyutu aracılığı ile verileri toplanmıştır. Öntest-sontest verileri arasındaki fark Wilcoxon İşaretli Sıralar testi ile SPSS-20 programında analiz edilmiştir. Katılımcılardan her oturum sonrasında toplanan açık uçlu sorulardan elde edilen veriler içerik analizi ile analiz edilmiştir. Yapılan analizler sonunda katılımcıların yaşam anlamı ölçeğinden, amaç bilinci ölçeğinden ve DAS-21 ölçeği depresyon alt boyutundan aldıkları ön test ve son test puanları arasındaki fark istatistiksel olarak anlamlı bulunmuştur. Söz konusu farklılık son testler lehine gerçekleşmiştir. Nitel verilerin analizinde katılımcıların yanıtlarının anlamlandırma, içsel yolculuk, farkındalık ve empati temaları etrafında şekillendiği görülmüştür.
The Use of Intermittent Non-Invasive Ventilation as an Alveolar Recruitment
Method for Patient with Severe COVID-19 Pneumonia
Authors: Zouwayhed Mazen , Gouher Saria , Bhaskar Balu , Zain Moeena , Burghleh Samer , Khani Rania , Yousef Razan , Khan Abdul Rahman , Aoun Naim
Published: December 2021

Background:

The use of non-invasive ventilation (NIV) as a therapy for acute respiratory distress syndrome (ARDS) secondary to COVID 19 pneumonia has
been controversial. NIV is an aerosol generating procedure which may increase the risk of viral transmission amongst patients and staff. Because
of fear of aerosolizing the virus and transmitting the disease, initial expert recommendation was to avoid NIV and proceed with early intubation.
With further experience of the virus, this recommendation has been challenged and NIV has been used widely with some retrospective studies
quoting between 11 to 56 percent of COVID 19 related respiratory failures being treated with NIV.

Objective:

The objective of this study is to assess the efficacy and safety of using non-invasive mechanical ventilation as an alveolar recruitment method for
patients with severe COVID 19 pneumonia. This method was used by our respiratory team on selected patients during the early phase of the
COVID 19 pandemic.

Methods:

We reviewed the charts of patients that were admitted to the American Hospital Dubai intensive care unit, or our medical step-down unit who had
diffuse bilateral infiltrates requiring oxygen supplementation between March and October 2020. We identified patients who were on intermittent
BiPAP in addition to standard care. We also monitored the rate of infection among staff taking care of these patients.

Results:

Average length of stay after starting BIPAP therapy was 6.8 days, while the average total length of stay was 13.6 days. Only one patient was
transferred to the ICU after being on the BIPAP protocol and did not need intubation.
All patients were discharged home either without oxygen or with their chronic baseline home oxygen requirement.
Radiological improvement in aeration was seen in 100% of patients at follow-up x-ray post-intervention.
There were no reported pulmonary complications from barotrauma, such as pneumothorax or pneumomediastinum.
There were no reported cases of staff infection to the health care workers that were taking care of these patients

Conclusion:

Our first of its kind observational study showed clearly that using BIPAP therapy for one hour three times daily during nebulization therapy in
addition to standard care resulted in a significant reduction in hospital length of stay and hastened the clinical and radiological improvement of
patients with severe COVID 19 pneumonia.
Methemoglobinaemia in Diabetic Ketoacidosis Patient
Authors: Mohamed Magdy , Javed Nadem
Published: December 2021

Background:

Glucose-6-phosphate dehydrogenase (G6PD) deficiency is a common X-linked genetic disorder.

Case Presentation:

In this paper, we report a case of a 41-years-old male patient with non-insulin-dependent diabetes and a family history of G6PD deficiency never
known to have any previous hemolytic episodes, presented as a case of diabetic ketoacidosis with features of hemolytic anemia due to G6PD
deficiency manifesting as methemoglobinemia and anemia.

Conclusion:

Our patient successfully managed with ascorbic acid and red blood cell transfusion. Clinicians should, therefore, be aware of the possibility of this
uncommon association between diabetic ketoacidosis, G6PD deficiency, and methemoglobinemia which may be present in patients with G6PD
deficiency and severe hemolysis.
Screening of Natural Compounds Against SOD1 as a Therapeutic Target
for Amyotrophic Lateral Sclerosis
Authors: Pahal Sonu , Chaudhary Amit , Singh Sangeeta
Published: December 2021

Background:

Amyotrophic lateral sclerosis (ALS) is an uncommon and progressive neurological
illness that predominantly includes the neurons liable for voluntary muscular activities. Starting from
weakness or stiffness in muscles, this gradually exploits the strength and ability to speak, eat, move, and
even breathe. Its exact mechanism is still not clear, but mutations in the SOD1 gene have been reported to
cause ALS, and some studies also found involvement of SOD1 overexpression in the pathogenesis of
ALS. As of now, there is no remedy available for its cure.

Objective:

The objective of this study is to identify the potential inhibitors for wild type 1HL5, l113T
mutant, and A4V mutant of SOD1 (Superoxide Dismutase 1) protein.

Methods:

In this study, in silico approaches like virtual screening, molecular docking, pharmacokinetic
parameters study, and molecular dynamics simulation were used to identify the best potential inhibitors
against wild type and mutant SOD1 protein.

Results:

On the basis of binding affinity and binding energy, the top three compounds
ZINC000095486263, ZINC000095485989, and ZINC000028462577 were observed as the best compounds.
In the case of 1HL5, ZINC000095486263 had the highest binding affinity with docking score -
10.62 Kcal/mol, 1UXM with ZINC000095485989 had the highest docking score -12.03 Kcal/mol, and
4A7V with ZINC000028462577 was found -11.72 Kcal/mol. Further, Molecular Dynamic Simulations
(MDS) results showed that the ZINC000095486263, ZINC000095485989, and ZINC000095485956
compounds were formed a stable complex with 1HL5, 1UXM, and 4A7V respectively.

Conclusions:

After analyzing the results, we hereby conclude that natural compounds such as
ZINC000095486263, ZINC000095485989, and ZINC000095485956 could be used as a potential inhibitor
of 1HL5, 1UXM, and 4A7V respectively for ALS treatment and could be used as a drug. Further, In
vivo/vitro study of these compounds could be a future direction in the field of drug discovery.
Computational Study Reveals PARP1 and P2Y1 Receptors as Prospective
Targets of Withaferin-A for Cardiovascular Diseases
Authors: Ravindran Rekha , Posa Krishnamoorthy Praveen Kumar , Kumar Sriram , Roy Sujata , Gowthaman Sakthi Abirami , Rajkumar Johanna
Published: December 2021

Background:

Cardiovascular Diseases (CVDs) remain the leading cause of death worldwide,
which urges for effective strategies of prevention and treatment. Withaferin-A (WFA), the key metabolite
identified in Withania somnifera, has been known for its cardioprotective properties. Although it has been
traditionally employed to treat cardiovascular ailments for several decades, its exact mechanism of action
still remains unexplained.

Objective:

The current study modeled and scored the interactions of WFA with nine prospective protein
targets associated with cardiovascular diseases through molecular docking and DSX-scoring.

Methods:

Molecular docking was carried out using Autodock and DSX scoring was carried out using
DSX standalone software. WFA was observed to favorably interact with six targets before DSX-based
rescoring, but only with Poly (ADP-Ribose) Polymerase-1 and P2Y Purinoceptor-1 after DSX-based
rescoring. The spatial orientation, physicochemical properties and structural features of Withaferin-A
were compared with that of the approved drugs by pharmacophore modeling and hierarchical clustering.

Results:

The results of molecular docking, DSX-based rescoring and complete pharmacophore modeling
together revealed that PARP1 and P2Y1 receptors could be prospective targets of WFA for the treatment
of CVD.

Conclusion:

Simulation using GROMACS has revealed that WFA forms a more stable complex with
PARP1 and will be useful in developing broad-spectrum drugs against cardiovascular diseases. Further
computational studies through machine learning and network pharmacology methods can be carried out to
improve the Withaferin-A compound features by incorporating additional functional groups necessary for
molecular recognition of the target genes in the network responsible for cardiovascular diseases.
Decitabine Enhances Acute Myeloid Leukemia Cell Apoptosis through SH3BGRL
Upregulation
Authors: Tong Xiuzhen , Wang Haihe , Chen Xiaomin , Liu Fengqi , Rong Dade , Xu Limei
Published: December 2021

Background:

SH3-domain-binding glutamic acid-rich protein-like protein (SH3BGRL) is downregulated in
acute myeloid leukemia (AML). Clinically, DNA demethylating drug decitabine (DAC) combined with traditional
chemotherapies reveals better efficacy on AML patients than the conventional chemotherapies alone. Our previous
results revealed that human SH3-domain-binding glutamic acid-rich protein-like protein (SH3BGRL) plays a tumor
suppressive role in AML but whether there is a connection between DAC and SH3BGRL expression remains elusive.

Methods:

Here, we tentatively treated AML cell lines U937, MV4, and HL-60 with DAC and Western Blots, RT-PCR
was used to detect the expression of SH3BGRL. Cell proliferation and apoptosis were determined using Annexin V/7-
AAD staining. Real-time RT-PCR and Western blot were used to determine the expression of SH3BGRL mRNA and
protein. Methylation-specific PCR was used to quantify the DNA methylation in AML cell lines.

Results:

DAC had cytotoxicity in HL-60, MV4, and U937. In U937 cell lines, treatment with DAC showed the upregulation
of cleaved caspase3, PARP, and SH3BGRL. Upon treatment, up-regulation of SH3BGRL mRNA and protein
was dose-dependent and this activity was partially inhibited in endogenous SH3BGRL knockdown cell lines.

Conclusion:

Thus, our results demonstrated a possibly cytotoxic role of DAC on AML cells by upregulation of
SH3BGRL expression at epigenetic modulation level and the methylation status in the SH3BGRL promoter region
could be a supplemental diagnostic marker to the precise administration of DAC to AML patients.
Review of Therapies using TiO2 Nanomaterials for Increased Anticancer Capability
Authors: Pérez-Larios Alejandro , Rodríguez-Barajas Noé , Anaya-Esparza Luis Miguel , Villagrán-de la Mora Zuami , Sánchez-Burgos Jorge Alberto
Published: December 2021

Recently, Titanium dioxide (TiO2) has been studied as an alternative to treat cancer diseases under different
activation therapies. The aim of this review was to describe the effect of TiO2 nanoparticles (NPs) on some cancer cell
lines and their interaction with phototherapies such as photodynamic therapy (PDT), photothermal therapy (PTT),
sonodynamic therapy (SDT), and ultraviolet therapy (UV) for anticancer treatment. The use of TiO2 combined with
PDT, PTT, SDT, or UV has shown a remarkable capacity to enhance the killing of cancer cells through reactive oxygen
species formation. Thus, the combination of TiO2 and activation therapies exhibited great potential and could be a
viable anticancer treatment strategy. However, more studies on phototherapies in combination with TiO2 and their
effects under different experimental conditions (TiO2 concentration, type of cancer cells, and intensity and frequency
of therapies) are necessary to guarantee the safe use of this kind of therapy.
The Regulatory Network Played by miRNAs During Normal Pregnancy and Preeclampsia: A Comparative Study
Authors: Ojeda-Casares Héctor , Paradisi Irene
Published: December 2021

Background:

Preeclampsia is a pregnancy-specific syndrome, characterized by hypertension, proteinuria, and edema. Affecting between 2% and 8% of gestations worldwide, it accounts for 10% to 15% of maternal deaths. Although its etiology remains unclear, it includes complex pathological processes involving microRNAs, small non-coding RNA molecules with post-transcriptional repression effects on target mRNAs.

Objective:

To assess the expression of miRNAs during normal pregnancies and those complicated by preeclampsia, a sample of Venezuelan women were studied.

Method:

Nine placental microRNAs (hsa-miR- 20a-5p, 21-3p, 26a-5p, 181a-5p, 199a-5p, 210-3p, 222-5p, 223-3p, 424-3p) were measured in maternal plasma during the second and third trimesters of normal pregnancies, using a SYBR Green®-based real-time PCR, and compared the results against women affected by preeclampsia.

Results:

All assessed miRNAs were detected in maternal plasma in pregnancies with and without preeclampsia. All except miR-222 were over-expressed during disease when compared to the second and to third-trimester controls. miR-20a, miR-21, miR-26a, and miR-223 were down-regulated in the third trimester in comparison to the second trimester in normal pregnancies.

Conclusion:

The variation of the miRNAs expression through normal pregnancies suggested their involvement in normal physiological pregnancy processes. In contrast, the significant deregulation of the nine studied miRNAs during preeclampsia indicated the involvement of their target genes in the pathogenesis of the disease. miR-199a and miR-21-3p showed the greatest changes in expression. This study shows for the first time the presence of miR-20a, miR-199, and miR-424 and the variations they undergo in the plasma of pregnant women with preeclampsia.
Analysis of Overexpressed miRNA in Circulation and Cancer Tissue to Develop a Potential microRNA Panel for the Diagnosis of Colorectal Cancer
Authors: Pruseth Babita , Ghosh Amit , Pradhan Dibyabhaba , Purkait Suvendu , Guttula Praveen Kumar
Published: December 2021

Background:

Colorectal cancer (CRC) represents the world’s fourth deadly cancer, but its early diagnosis can be curative with considerable success rates. This study was aimed to identify CRC specific microRNAs (miRNAs) in tissue and serum samples to develop a miRNA-based diagnostics panel for the minimal invasive detection of CRC in early condition.

Methods:

By integrating four microarrays in tissue and serum samples of CRC from Gene Expression Omnibus (GEO) database, we screened out the highly expressed miRNAs in each dataset by using limma R package. Two important upregulated miRNAs namely hsa-miR-1246 and hsa-miR-1825 were overlapped in both tissue and serum samples of CRC, and were investigated to target identification, followed by functional annotation and protein- protein interaction (PPI) study for the target genes through DAVID and STRING respectively. Finally, hub target genes were retrieved by Cytoscape analysis.

Results:

It was shown that target genes of hsa-miR-1246 and hsa-miR-1825 were involved with core KEGG pathways (such as cAMP, PI3K-Akt and calcium signaling pathway). In addition, biological processes (such as cell adhesion and cell proliferation), cellular components (such as plasma membrane and cytosol), molecular functions (such as protein binding and metal ion binding), were mostly associated with the target genes. Their top 5 target genes were retrieved and their biological function towards tumor progression was shown using Cancer Hallmarks Analytics Tool.

Conclusion:

This study suggested that hsa-miR-1246 and hsa-miR-1825, as overlapped upregulated tissue and circulating miRNAs might have a vital role in the development of CRC and their five hub target genes were identified.
A Review on Mechanical Properties of Natural Fibre Reinforced PLA
Composites
Authors: Kumar Anil , Sinha Agnivesh Kumar , Rao Kasi Raja , Soni Vinay Kumar , Chandrakar Rituraj , Sharma Hemant Kumar
Published: December 2021

Presently, scientists and researchers are in an endless quest to develop green, recyclable,
and eco-friendly materials. Natural fibre reinforced polymer composites became popular
among materialists due to their lightweight, high strength-to-weight ratio, and biodegradability.
However, all-natural fibre reinforced polymer composites are not biodegradable. Polymer matrices
like poly-lactic acid (PLA) and poly-butylene succinate (PBS) are biodegradable, whereas
epoxy, polypropylene, and polystyrene are non-biodegradable polymer matrices. Besides biodegradability,
PLA has been known for its excellent physical and mechanical properties. This review
emphasises the mechanical properties (tensile, flexural, and impact strengths) of natural fibrereinforced
PLA composites. Factors affecting the mechanical properties of PLA composites are
also discussed. It also unveils research gaps from the previous literature, which shows that limited
studies are reported based on modeling and prediction of mechanical properties of hybrid PLA
composites reinforcing natural fibres like abaca, aloe vera, and bamboo fibres.
Pengaruh Konsentrasi Dan Jenis Gelling Agent Terhadap Sifat Fisikokimia Jelly Drink Jeruk Bali (Citrus Maxima)
Authors:   Universitas Ahmad Dahlan Yogyakarta, Nugiharti Isti , Haryadi Hari ,   Universitas Ahmad Dahlan Yogyakarta
Published: December 2021

Jeruk bali (Citrus maxima) mengandung vitamin C 26.36 mg/100 g dan serat 0.32 % b/b Namun, jeruk bali mengandung tanin dan saponin yang menyebabkan adanya rasa pahit pada jeruk bali. Pengolahan jeruk bali menjadi jelly drink diharapkan dapat membantu memenuhi kebutuhan vitamin C dan serat pangan yang berasal dari gelling agent yang ditambahkan. Penelitian ini bertujuan untuk mengetahui pengaruh konsentrasi dan jenis gelling agent terhadap sifat fisikokimia jelly drink jeruk bali serta mengetahui formulasi jelly drink jeruk bali dengan kadar serat dan vitamin C tertinggi. Penelitian ini menggunakan 2 faktor yaitu konsentrasi gelling agent 0.8%, 1%, dan 1.2% serta jenis gelling agent yaitu kappa karagenan dan jelly powder. Sifat fisikokimia yang diuji adalah vitamin C, serat, viskositas, kadar air, dan pH dengan uji statistik two-way anova. Hasil penelitian menunjukkan bahwa konsentrasi gelling agent mempengaruhi sifat fisikokimia jelly drink jeruk bali. Sedangkan, jenis gelling agent mempengaruhi kadar serat, viskositas, dan pH jelly drink jeruk bali. Kadar vitamin C dan serat pangan tertinggi terdapat pada formulasi penambahan kappa karagenan 1.2% dengan vitamin C sebesar 69.27% g/ml dan serat pangan sebesar 0.794% b/b.
OPTIMASI PRODUKSI SENYAWA VANILIN DARI BIOKONVERSI LIGNOSELULOSA TANDAN KOSONG KELAPA SAWIT DENGAN PHANEROCHAETE CHRYSOSPORIUM MENGGUNAKAN RESPONSE SURFACE METHOD
Authors: Rohmah Rohmah , Suhartini Sri , Nurika Irnia
Published: December 2021

Limbah tandan kosong kelapa sawit (TKKS) merupakan limbah hasil produksi minyak sawit yang memiliki kandungan lignoselulosa (lignin, selulosa, hemiselulosa) tinggi, dapat dimanfaatkan untuk produksi senyawa aromatik vanilin dengan menggunakan jamur Phanerochaete chrysosporium dan pelarut etil asetat. Diketahui pelarut etil asetat dan lama ekstraksi berpengaruh terhadap kadar vanilin dan yield vanilin dengan Response Surface Methode (RSM) terdiri dari 4 faktor yang digunakan untuk mengukur sejauh mana faktor-faktor tersebut saling berpengaruh terhadap kadar vanilin. Regresi terdiri dari 13 titik faktorial dengan 6 titik aksial, dan 7 titik tengah melalui softwere Design Expert 7.0.0. Faktor-faktor optimasi digabung untuk mendapatkan nilai optimum untuk menghasilkan kadar dan yield vanilin yang bagus. Hasil penelitian optimasi RSM volume pelarut etil asetat yaitu 100,36 mL dan lama ekstraksi yaitu 120,27 menit yang digunakan dalam penelitian TKKS dengan nilai validasi optimal yang didapatkan yaitu kadar vanilin mendekati hasil prediksi sebesar 0,015% dan yield vanilin sebesar 11,862 µg/g. Hasil dalam penelitian ini menunjukkan penggunaan bio-pretreatment Phanaerocaete chrysosporium sebagai biokonversi TKKS menghasilkan vanilin dapat menekan biaya besar dan meningkatkan nilai fungsi dari limbah TKKS
PENAMBAHAN BUBUK EKSTRAK KUBIS MERAH (BRASSICA OLERACEAE VAR. CAPITATA F. RUBRA) SEBAGAI SUMBER ANTIOKSIDAN DAN PEWARNA ALAMI PADA CHEESECAKE
Authors: Nidyasari Wuwur Rosalia , Reni Swasti Yuliana , Sinung Pranata Franciscus
Published: December 2021

Antosianin merupakan pigmen warna pada tumbuhan yang memiliki aktivitas antioksidan. Antosianin dapat ditemukan pada tumbuhan yang berwarna biru atau merah seperti kubis merah. Upaya untuk meningkatkan antioksidan pada bahan pangan salah satunya dengan cara menambahkan bubuk ekstrak antosianin dari tanaman ke dalam bahan pangan sebagai pewarna alami. Produk makanan yang dapat diberi tambahan pewarna salah satunya adalah kue. Cheesecake yang diberi tambahan bubuk ekstrak kubis merah diharapkan dapat disukai karena memiliki penampilan yang menarik dan memiliki manfaat sebagai antioksidan. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh penambahan bubuk ekstrak kubis merah terhadap kualitas kimia, fisik, mikrobiologi dan organoleptik cheesecake serta mengetahui konsentrasi terbaik penambahan bubuk ekstrak kubis merah. Rancangan percobaan yang digunakan pada penelitian ini adalah Rancangan Acak Lengkap (RAL) dengan variasi bubuk ekstrak kubis merah 0% (kontrol), 5% (A), 10% (B), dan 15% (C). Hasil penelitian ini menunjukkan bahwa cheesecake dengan variasi penambahan bubuk ekstrak kubis merah mempunyai kadar air 42,38-49,06%, kadar abu 0,64-0,84%, kadar protein 4,57-4,81%, kadar lemak 27,17-31,85%, kadar karbohidrat 13,98-24,90%, total fenolik 0,76-1,16 mg GAE/g DW, aktivitas antioksidan 27,27-55,58%, total asam tertitrasi (TAT) 0,01-0,02%, dan uji mikrobiologi yang terdiri dari uji Salmonella dan angka lempeng total yang sudah memenuhi standar yang ditetapkan oleh USDA (United State Department of Agriculture) dan BSN (Badan Standardisasi Nasional). Hasil organoleptik menunjukkan bahwa cheesecake A (5%) merupakan produk yang paling disukai oleh panelis
Recent developments in reactivity testing of supplementary cementitious materials
Authors: Suraneni Prannoy
Published: December 2021

Identification and rapid characterization of novel supplementary cementitious materials (SCMs) is a critical need, driven by shortfalls in conventional SCMs. In this study, we present a discussion of recently developed reactivity tests – the R3 test, the modified R3 test, the lime strength test, and the bulk resistivity index test. These tests measure reactivity parameters such as heat release, bound water, calcium hydroxide consumption, strength, and bulk resistivity. All tests can screen inert from reactive materials. To additionally differentiate pozzolanic and latent hydraulic materials, two parameters, for example, calcium hydroxide consumption and heat release, are needed. The influences of SCM bulk chemistry, amorphous content, and fineness on measured reactivity are outlined. Reactivity test outputs can predict strength and durability of cement paste/mortar/concrete; however, caution must be exercised as these properties are influenced by a variety of other factors independent of reactivity. Thoughts are provided on using reactivity tests to screen materials for concrete durability.
A bibliometric analysis of the supply chain finance research
Authors:   International Economics Faculty, Banking University, Ho Chi Minh City, Vietnam, Sang Nguyen Minh
Published: December 2021

The purpose of the article is to use biometric methods to perform a high-level analysis of research trend analysis on supply chain finance through 305 studies in the field of Business-Economics and Social Sciences published on the Scopus database for the period 2006-2021. The findings provide an overview of worldwide publication trends on the topic of supply chain finance, specifically: (i) the most cited studies; (ii) the most cited authors; (iii) the most influential journals; (iv) the main research keywords among which the network links; (v) leading research institutions and (vi) research collaboration trends among countries on supply chain finance. The study provides more scientific evidence about the current big picture of publication trends in the world, thereby suggesting and recommending future research directions on supply chain finance.
A critical analysis about the supposed role of azithromycin in the treatment of covid-19
Authors: Santana Ricardo Ruan , Barbosa Bárbara Oliva , Soares José Rivaldo de Oliveira , Colombo Rayssa Mielo , Santos Victória Rafaela , Amaral Ricardo Guimarães , Andrade Luciana Nalone
Published: December 2021

After over one year, the coronavirus disease 2019 (covid-19) has still affected millions of people. For this reason, global efforts to promote better treatment of covid-19 have been undertaken focused on the repurposing of existing medications.In Brazil, azithromycin, a broad-spectrum antibiotic, has been used in association with other drugs as an immunomodulatory, anti-inflammatory, and anti-viral agent, regardless of bacterial co-infection. Indeed, data from experimental studies have demonstrated the capacity of this drug in reducing the production of infection-induced pro-inflammatory cytokines, such as IL-8, IL-6, and TNF-alpha. However, observational studies revealed conflicting results regarding its effect, whereas well-conducted clinical trials have not shown a considerable effect of this agent on the improvement of clinical outcomes. This narrative review addressed the possible role of this antibiotic in the management of covid-19, based on data from clinical and preclinical studies.
Stem cell therapy in vascular diseases
Authors: Navarro Túlio Pinho , Lopes Lara Lellis Navarro Minchillo , Dardik Alan
Published: December 2021

Despite scientific advances, vascular diseases are responsible for one third of deaths. Understanding the biology of stem cells and cell therapy can mean an important advance in reducing this mortality.
The pathogenetic significance of endothelial dysfunction in the development of diabetic nephropathy in patients with type 2 diabetes mellitus
Authors: Reznikova E. A. , Gordeeva M. A. , Babaeva A. R.
Published: December 2021

Objective: to determine the level of circulating markers of endothelial dysfunction (endothelin‑1, von Willebrand factor (vfV), endothelial NO-synthase (e‑NOS)) in the blood serum of patients with type 2 diabetes mellitus (DM), as well as to assess the pathogenetic significance endothelial dysfunction in the development of diabetic nephropathy.

Materials and methods: the study included 93 patients with type 2 DM, including 28 men (30.1%) and 65 women (69.9%), aged 30 to 79 years, the average age of patients was 59.7±8.4 of the year. The main group included patients with both newly diagnosed type 2 DM and a long-term diabetic history. The duration of the disease averaged 9.5±7.5 years. The majority of patients with DM type 2 (92.5%) at the time of inclusion in the study had various variants of microvascular complications of diabetes, only a small number of patientsin this group (7.5%) had no signs of diabetic angiopathy. Signs of various stages of diabetic nephropathy were observed in 60 patients (69.2%). The comparison group consisted of 30 patients with essential arterial hypertension, including 12 men (40%) and 18 women (60%), aged 34 to 70 years, on average 56.1 ± 8.1 years. The control group consisted of 32 apparently healthy individuals. In all patients, along with routine methods of clinical, laboratory and instrumental examination, the level of circulating markers of endothelial dysfunction (endothelin‑1, von Willebrand factor (vWF), endothelial NO-synthase (e‑NOS)) in blood serum was measured using enzyme-linked immunosorbent assay (ELISA).

Results: in patients with DM type 2 and diabetic nephropathy, a statistically significant increase in the concentration of circulating markers of endothelial dysfunction was revealed in comparison with hypertensive patients and healthy individuals. An increase in the level of endothelin‑1 relative to the borderline reference value was found in 73 (78.5±4.1%), vfV in 63 (67.7±4.8%) and e‑NOS in 65 (69.9±4.7%) of patients with DM type 2. In the groups of participants with hypertension and healthy individuals, endothelial imbalance was noted by us significantly less often than in patients with DM type 2, the levels of endothelin‑1 and vfV in people with hypertension were increased in more cases than in healthy individuals. It was noted that the levels of circulating markers of endothelial dysfunction increase with an increase in the duration of DM type 2, and also significantly increase under conditions of carbohydrate decompensation.

Conclusion: the results obtained confirm the pathogenetic role of endothelial dysfunction in the development of diabetic nephropathy in patients with type 2 diabetes, increasing endothelial imbalance in persons with a long diabetic history and lack of compensation for carbohydrate metabolism.
Rare anomaly of kidney development – L-shaped fusion
Authors: Batiushin M. M. , Blinov I. M. , Bondarenko N. B. , Stephanova E. D. , Batiushina A. M.
Published: December 2021

Within the framework of the publication, an L‑shaped fusion of the kidneys (horseshoe‑shaped) is considered, referring to the category of asymmetric fusion. The presented clinical case is an extremely rare form of congenital anomaly of renal fusion, demonstrating the difficulty of verifying the correct diagnosis. The article presents the results of a tomographic study, which, in addition to enlargement of the kidneys, recorded signs of dysplasia of the left ureter (stricture) and signs of an arterial vascular bed (aberrant artery of the upper part of the L‑shaped horseshoe).