bluuebunny/crossref_metadata_2025 huggingface dataset:
Osmotic Adjustment in Wheat (Triticum aestivum L.) During Pre- and Post-anthesis Drought
Authors: Verbeke Sarah , Padilla-Díaz Carmen María , Haesaert Geert , Steppe Kathy
Published: January 2022

Pre-anthesis drought is expected to greatly increase yield losses in wheat (Triticum aestivum L.), one of the most important crops worldwide. Most studies investigate the effects of pre-anthesis drought only at maturity. The physiology of the plant before anthesis and how it is affected during drought is less studied. Our study focused on physiological patterns in wheat plants during pre- and post-anthesis drought. To this end, we measured leaf xylem water potential, osmotic potential and water content in different plant parts at a high temporal frequency: every 3 days, three times a day. The experiment started just before booting until 2 weeks after flowering. Drought stress was induced by withholding irrigation with rewatering upon turgor loss, which occurred once before and once after anthesis. The goal was to investigate the patterns of osmotic adjustment, when it is used for protection against drought, and if the strategy changes during the phenological development of the plant. Our data gave no indication of daily osmotic adjustment, but did show a delicate control of the osmotic potential during drought in both leaves and stem. Under high drought stress, osmotic potential decreased to avoid further water loss. Before anthesis, rewatering restored leaf water potential and osmotic potential quickly. After anthesis, rewatering restored water potential in the flag leaves, but the osmotic potential in the stem and flag leaf remained low longer. Osmotic adjustment was thus maintained longer after anthesis, showing that the plants invest more energy in the osmotic adjustment after anthesis than before anthesis. We hypothesize that this is because the plants consider the developing ear after anthesis a more important carbohydrate sink than the stem, which is a carbohydrate sink before anthesis, to be used later as a reserve. Low osmotic potential in the stem allowed turgor maintenance, while the low osmotic potential in the flag leaf led to an increase in leaf turgor beyond the level of the control plants. This allowed leaf functioning under drought and assured that water was redirected to the flag leaf and not used to refill the stem storage.
A Study on Environmental, Social and Governance Fund Performance and Fund Flow: Evidence From Korea Stock Exchange
Authors: Kwak Dongchul , Kim Yu Kyum , Kwon Il Sook
Published: January 2022

This study analyzed the sensitivity between fund flow and fund performance with Korean funds, whether there would be a difference in the sensitivity between environmental, social and governance (ESG) funds and non-ESG funds, and whether there was a difference in sensitivity according to the type of past fund performance (positive and negative). The main results of the analysis are as follows. First, the analysis of the fund flow–performance correlation of Korean funds revealed that they had a negative (−) correlation and the ESG did not affect fund flow. Analysis of the difference in sensitivity between fund flow and performance volatility revealed that there was a negative (−) correlation regardless of the performance measuring method and ESG. Finally, the comparison of fund flow and performance sensitivity according to the type of past fund performance revealed that despite consistent asymmetry, there was little difference in sensitivity asymmetry between ESG funds and non-ESG funds. The results reveal that, unlike the expectation that investors in Korean ESG funds would focus more on non-financial properties like the purpose of investment than on profit, they attach the same importance to fund performance.
The Bidirectional Social-Cognitive Mechanisms of the Social-Attention Symptoms of Autism
Authors: Mundy Peter , Bullen Jenifer
Published: January 2022

Differences in social attention development begin to be apparent in the 6th to 12th month of development in children with Autism Spectrum Disorder (ASD) and theoretically reflect important elements of its neurodevelopmental endophenotype. This paper examines alternative conceptual views of these early social attention symptoms and hypotheses about the mechanisms involved in their development. One model emphasizes mechanism involved in the spontaneous allocation of attention to faces, or social orienting. Alternatively, another model emphasizes mechanisms involved in the coordination of attention with other people, or joint attention, and the socially bi-directional nature of its development. This model raises the possibility that atypical responses of children to the attention or the gaze of a social partner directed toward themselves may be as important in the development of social attention symptoms as differences in the development of social orienting. Another model holds that symptoms of social attention may be important to early development, but may not impact older individuals with ASD. The alterative model is that the social attention symptoms in infancy (social orienting and joint attention), and social cognitive symptoms in childhood and adulthood share common neurodevelopmental substrates. Therefore, differences in early social attention and later social cognition constitute a developmentally continuous axis of symptom presentation in ASD. However, symptoms in older individuals may be best measured with in vivo measures of efficiency of social attention and social cognition in social interactions rather than the accuracy of response on analog tests used in measures with younger children. Finally, a third model suggests that the social attention symptoms may not truly be a symptom of ASD. Rather, they may be best conceptualized as stemming from differences domain general attention and motivation mechanisms. The alternative argued for here that infant social attention symptoms meet all the criteria of a unique dimension of the phenotype of ASD and the bi-directional phenomena involved in social attention cannot be fully explained in terms of domain general aspects of attention development.
Risk Factors for Acute Brucellosis in Patients on the Day of Admission at Selected Hospitals of Abbottabad, Pakistan
Authors: Hassan Laiba , Ali Shahzad , Syed Muhammad Ali , Shah Asim Ali , Abbasi Shahid Ahmad , Tabassum Sadia , Saeed Usama , Melzer Falk , Khan Aman Ullah , El-Adawy Hosny , Neubauer Heinrich
Published: January 2022

Brucellosis is a neglected zoonotic disease of ruminants. It causes severe health problems in humans and significant economic loss. Only a limited number of studies have been conducted in Pakistan to determine the prevalence of human brucellosis and related risk factors. The objectives of the current cross-sectional study were to determine the prevalence of anti-Brucella antibodies in sera collected from symptomatic patients at three hospitals of Abbottabad using a commercial slide agglutination test (SAT) and to determine risk factors for brucellosis for these patients. Five hundred blood samples were collected. A questionnaire was filled in for each patient to obtain information on age, gender, living area, brucellosis associated symptoms, associated risk factors, pregnancy and abortion history. A total of 13.6% (n = 68) patients were found to be SAT positive and in 83.3% (n = 57) of these samples Brucella DNA was detected by genus specific RT-PCR for BCSP-31 gene. Statistical analysis was performed to determine odd ratios, risk ratios, 95% confidence intervals, and p-values. The prevalence of brucellosis by SAT was reported to be higher in women (14.6%, n = 44) than in men (12.1%, n = 24). The age group 25–50 years was found to be at higher risk for brucellosis (14.5%, n = 50) “animal contact” was reported as the main risk factor followed by “consumption of raw animal products.” Out of 131 pregnant women and 21 patients had abortion, the seropositivity of Brucellosis was 9.9% and 23.8%, respectively. The present study reports a striking prevalence of brucellosis among patients including pregnant women at three hospitals of Abbottabad. These findings may foster strategies for controlling human brucellosis at household level, raising of awareness about brucellosis in hospital and family doctors, and finally in setting up an eradication program in the dairy industry.
COVID-19 Infection, the COVID-19 Pandemic, and Changes in Sleep
Authors: Donzella Sidney M. , Kohler Lindsay N. , Crane Tracy E. , Jacobs Elizabeth T. , Ernst Kacey C. , Bell Melanie L. , Catalfamo Collin J. , Begay Rachelle , Pogreba-Brown Kristen , Farland Leslie V.
Published: January 2022

The objective of this study was to investigate the differences in sleep patterns among individuals with and without laboratory-confirmed SARS-CoV-2 infection. Laboratory-confirmed SARS-CoV-2 test results and self-reported measures recalling sleep habits prior to and during the pandemic were collected from May 2020 to March 2021 among 1,848 individuals in The Arizona CoVHORT Study. We used linear and logistic regression to model the association between test status, presentation of symptoms, and time since test result with sleep duration and trouble sleeping, respectively. Mixed models were used to investigate change in sleep duration prior to the pandemic compared to during the pandemic. Overall, 16.2% of the sample were SARS-CoV-2 positive, 64.3% were SARS-CoV-2 negative, and 19.5% were untested for SARS-CoV-2. Independent of SARS-CoV-2 infection status, all participants slept longer during the pandemic compared to pre-pandemic (Δ SARS-CoV-2 positive: 77.7 min, 95% CI 67.9, 87.5; Δ SARS-CoV-2 negative: 13.4 min, 95% CI 8.4, 18.3). However, SARS-CoV-2 positive participants slept 60.9 min longer (95% CI 49.1, 72.8) than SARS-CoV-2 negative participants in multivariable-adjusted models and had greater odds of trouble sleeping three or more times per week since the start of the pandemic (OR: 1.34 95% CI 1.02, 1.77) This greater odds of trouble sleeping persisted for participants who reported sleep habits > 30 days after their positive SARS-CoV-2 (OR: 2.11 95% CI 1.47, 3.03). Sleep patterns among non-hospitalized individuals with COVID-19 were altered following infection, regardless of the presentation of symptoms and time since infection.
The Menstrual Endometrium: From Physiology to Future Treatments
Authors: Watters Marianne , Martínez-Aguilar Rocío , Maybin Jacqueline A.
Published: January 2022

Abnormal uterine bleeding (AUB) is experienced by up to a third of women of reproductive age. It can cause anaemia and often results in decreased quality of life. A range of medical and surgical treatments are available but are associated with side effects and variable effectiveness. To improve the lives of those suffering from menstrual disorders, delineation of endometrial physiology is required. This allows an increased understanding of how this physiology may be disturbed, leading to uterine pathologies. In this way, more specific preventative and therapeutic strategies may be developed to personalise management of this common symptom. In this review, the impact of AUB globally is outlined, alongside the urgent clinical need for improved medical treatments. Current knowledge of endometrial physiology at menstruation is discussed, focusing on endocrine regulation of menstruation and local endometrial inflammation, tissue breakdown, hypoxia and endometrial repair. The contribution of the specialised endometrial vasculature and coagulation system during menstruation is highlighted. What is known regarding aberrations in endometrial physiology that result in AUB is discussed, with a focus on endometrial disorders (AUB-E) and adenomyosis (AUB-A). Gaps in existing knowledge and areas for future research are signposted throughout, with a focus on potential translational benefits for those experiencing abnormal uterine bleeding. Personalisation of treatment strategies for menstrual disorders is then examined, considering genetic, environmental and demographic characteristics of individuals to optimise their clinical management. Finally, an ideal model of future management of AUB is proposed. This would involve targeted diagnosis of specific endometrial aberrations in individuals, in the context of holistic medicine and with due consideration of personal circumstances and preferences.
White Dwarfs as Physics Laboratories: Lights and Shadows
Authors: Isern J. , Torres S. , Rebassa-Mansergas A.
Published: January 2022

The evolution of white dwarfs is essentially a gravothermal process of cooling in which the basic ingredients for predicting their evolution are well identified, although not always well understood. There are two independent ways to test the cooling rate. One is the luminosity function of the white dwarf population, and another is the secular drift of the period of pulsation of those individuals that experience variations. Both scenarios are sensitive to the cooling or heating time scales, for which reason, the inclusion of any additional source or sink of energy will modify these properties and will allow to set bounds to these perturbations. These studies also require complete and statistical significant samples for which current large data surveys are providing an unprecedented wealth of information. In this paper we review how these techniques are applied to several cases like the secular drift of the Newton gravitational constant, neutrino magnetic moments, axions and weakly interacting massive particles (WIMPS).
A Literature Review of the Use of Weeds and Agricultural and Food Industry By-Products to Feed Farmed Crickets (Insecta; Orthoptera; Gryllidae)
Authors: Kuo Camille , Fisher Brian L.
Published: January 2022

Global socioeconomic systems exacerbate disparities that leave a disturbing proportion of the human population malnourished, making conventional food sources such as animal livestock unsustainable at global scales. Edible insects have the potential to solve both malnourishment and the unsustainability of conventional livestock agriculture. The sustainability and accessibility of farmed, edible insects, however, depends on their feed source. Future expansion of farming rests on developing locally available and affordable insect feeds. This article presents a literature review of studies which evaluate the performance of edible crickets (Orthoptera) in response to alternative feed sources such as weeds and the byproducts of agricultural and food industries. For each insect species evaluated, we analyze measurements of feed and insect performance, feed composition, effects of diet on cricket growth and survivorship, as well as other sources of performance differences. The aim of this review is to assess the current state of alternative feed research for farmed crickets and to provide guidelines for future studies on alternative feeds.
Evaluation of Implementation Effect of Cervical Cancer Comprehensive Treatment Patients With Whole-Course High-Quality Care Combined With Network Continuation Care
Authors: Chen Jing , Bai Hui
Published: January 2022

Purpose

Discuss the implementation effect of cervical cancer comprehensive treatment patients applying whole-course high-quality care combined with network continuation care.

Methods

From August 2020 to August 2021, 120 patients who met the inclusion criteria for comprehensive treatment of cervical cancer were divided into the regular group (n = 60) who received conventional care and the joint group (n = 60) who received whole-course high-quality care combined with network continuation care, according to the method of care. The comprehensive treatment cognition level, comprehensive treatment compliance, adverse reaction rate, quality of life questionnaire (QLQ-C30) score, self-rating anxiety/depression scale (SAS/SDS) score, and nursing satisfaction were compared between the two groups.

Results

After care, the comprehensive treatment cognition score and comprehensive treatment compliance score were higher in the joint group than in the regular group (P < 0.05). After care, the incidence of radiation cystitis and radiation proctitis was lower in the joint group than that in the regular group (P < 0.05). After care, QLQ-C30 scores on symptom domains, functional domains, and single questions were higher in both groups than before care, and were higher in the joint group than in the regular group (P < 0.05). After care, SAS and SDS scores were lower in both groups than before care, and were lower in the joint group than in the regular group (P < 0.05). After care, the joint group was more satisfied with care than the regular group (P < 0.05).

Conclusion

The implementation of cervical cancer comprehensive treatment patients with whole-course high-quality care combined with network continuation care has an ideal implementation effect, which can significantly increase the patient's cognition and compliance with treatment, the incidence of adverse reactions is less, the quality of life and emotional state have also improved significantly, and care satisfaction has also increased accordingly.
The Transcriptional Regulator MucR, but Not Its Controlled Acid-Activated Chaperone HdeA, Is Essential for Virulence and Modulates Surface Architecture and Properties in Brucella ovis PA
Authors: Tartilán-Choya Beatriz , Sidhu-Muñoz Rebeca S. , Vizcaíno Nieves
Published: January 2022

Brucella ovis is a non-zoonotic bacterium causing contagious epididymitis and other genital lesions in rams and responsible for significant economic losses in sheep-breeding areas. It is a naturally rough (without O-chains in the lipopolysaccharide) Brucella species whose virulence mechanisms have been less explored than those of zoonotic smooth brucellae (bearing O-chains that mask other outer membrane molecules). Considering the rough nature of Brucella ovis, the influence of surface components other than O-chains on its biological properties may be greater than in smooth Brucella species. Here we describe the construction and characterization of the mucR deletion mutant of virulent B. ovis PA, which is defective in a transcriptional regulator, affecting surface properties and virulence in smooth brucellae. This mutant showed increased amounts of three proteins identified as HdeA (acid-activated chaperone), Omp25d (outer membrane protein undetectable in the parental strain), and BOV_A0299 (hypothetical protein of unknown function). This observation correlated with the enhanced transcription of the corresponding genes and constitutes the first report on this type of proteome alteration in Brucella ΔmucR mutants. The upstream regions of the three genes contained AT rich domains with T-A steps described as binding sites for MucR in the Brucella abortus 2308 babR promoter (gene also upregulated in B. ovis ΔmucR), which suggests that hdeA, omp25d, and BOV_A0299 expression could be repressed by MucR through a direct binding to their promoter regions. Relative quantification of transcripts of several other genes selected according to the transcriptome of smooth brucellae ΔmucR mutants revealed not only similarities but also relevant differences among strains, such as those detected in flagellar and virB genes. Periplasmic HdeA has been related to the resistance of B. abortus to acidic pH, conditions encountered by Brucella inside phagocytes, but the deletion of hdeA in B. ovis PA and the ΔmucR mutant did not modify any of the evaluated properties of these strains. The B. ovis PA ΔmucR and ΔmucRΔhdeA mutants had defective in vitro growth and altered surface properties and architecture, exemplified by detectable amounts of Omp25d. Moreover, they showed virulence attenuation but established persistent splenic infection in mice, which encourages their evaluation as specifical attenuated vaccines against B. ovis.
Goji Berries Supplementation in the Diet of Rabbits and Other Livestock Animals: A Mini-Review of the Current Knowledge
Authors: Agradi Stella , Draghi Susanna , Cotozzolo Elisa , Barbato Olimpia , Castrica Marta , Quattrone Alda , Sulce Majlind , Vigo Daniele , Menchetti Laura , Ceccarini Maria Rachele , Andoni Egon , Riva Federica , Marongiu Maria Laura , Curone Giulio , Brecchia Gabriele
Published: January 2022

In the last decades, several nutraceutical substances have received great attention for their potential role in the prevention and treatment of different diseases as well as for their beneficial effects in promoting the health of humans and animals. Goji berries (GBs) are the fruit of Lycium barbarum and other species of Lycium, used in traditional Chinese medicine, and they have recently become very popular in the Occidental world because of their properties, such as anti-aging, antioxidant, anticancer, neuroprotective, cytoprotective, antidiabetic, and anti-inflammatory activities. These effects are essentially evaluated in clinical trials in humans; in experimental animal models, such as mice and rats; and in cell lines in in vitro studies. Only recently has scientific research evaluated the effects of GBs diet supplementation in livestock animals, including rabbits. Although studies in the zootechnical field are still limited and the investigation of the GB mechanisms of action is in an early stage, the results are encouraging. This review includes a survey of the experimental trials that evaluated the effects of the GBs supplementation on reproductive and productive performances, immune system, metabolic homeostasis, and meat quality principally in the rabbit with also some references to other livestock animal species. Evidence supports the idea that GB supplementation could be used in rabbit breeding, although future studies should be conducted to establish the optimal dose to be administered and to assess the sustainability of the use of GBs in the diet of the rabbit.
Zinc Methionine Improves the Growth Performance of Meat Ducks by Enhancing the Antioxidant Capacity and Intestinal Barrier Function
Authors: Chang Yaqi , Tang Huangyao , Zhang Zhenyu , Yang Ting , Wu Bing , Zhao Hua , Liu Guangmang , Chen Xiaoling , Tian Gang , Cai Jingyi , Wu Fali , Jia Gang
Published: January 2022

This study was conducted to investigate the effects of zinc methionine (Zn-Met) on the growth performance, antioxidant capacity and intestinal barrier function of meat ducks. Three hundred and sixty 1-day-old male Cherry Valley ducks were randomly divided into 6 groups with 6 replicates (10 birds each), and fed diets with 0, 30, 60, 90, 120 or 150 mg/kg Zn for 35 d. The results indicated that dietary supplementation with Zn-Met substantially increased the average daily gain (ADG), and reduced the feed to gain ratio (F/G) during 1–35 d (P < 0.05). Dietary Zn-Met markedly increased the activity of superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH), and reduced the malondialdehyde (MDA) content in the jejunum (P < 0.05). The mRNA expression levels of critical antioxidant enzymes such as SOD, CAT, and nuclear factor erythroid 2-related factor 2 (Nrf2) were increased by Zn in the jejunum (P < 0.05). Supplementation with 60, 90, 120, and 150 mg/kg of Zn significantly reduced the diamine oxidase (DAO) activity in the serum (P < 0.05). Different levels of Zn can increase the mRNA expression of occluding (OCLN) and zonula occludens-1 (ZO-1) in the jejunum (P < 0.05). Diets supplemented with zinc significantly increased the content of mucin2 (MUC2), secretory immunoglobulin A (sIgA), immunoglobulin A (IgA) and immunoglobulin G (IgG) in the jejunum of meat ducks (P < 0.05). The 16S rRNA sequence analysis indicated that 150 mg/kg of Zn had a higher relative abundance of Verrucomicrobia and Akkermansia in cecal digesta (P < 0.05). In conclusion, Zn-Met improved the growth performance of meat ducks by enhancing intestinal antioxidant capacity and intestinal barrier function. This study provides data support for the application of Zn-Met in meat duck breeding.
Adaptive Authentication Protocol Based on Zero-Knowledge Proof
Authors: Chistousov Nikita Konstantinovich , Kalmykov Igor Anatolyevich , Dukhovnyj Daniil Vyacheslavovich , Kalmykov Maksim Igorevich , Olenev Aleksandr Anatolyevich
Published: January 2022

Authentication protocols are expanding their application scope in wireless information systems, among which are low-orbit satellite communication systems (LOSCS) for the OneWeb space Internet, automatic object identification systems using RFID, the Internet of Things, intelligent transportation systems (ITS), Vehicular Ad Hoc Network (VANET). This is due to the fact that authentication protocols effectively resist a number of attacks on wireless data transmission channels in these systems. The main disadvantage of most authentication protocols is the use of symmetric and asymmetric encryption systems to ensure high cryptographic strength. As a result, there is a problem in delivering keys to the sides of the prover and the verifier. At the same time, compromising of keys will lead to a decrease in the level of protection of the transmitted data. Zero-knowledge authentication protocols (ZKAP) are able to eliminate this disadvantage. However, most of these protocols use multiple rounds to authenticate the prover. Therefore, ZKAP, which has minimal time costs, is developed in the article. A scheme for adapting protocol parameters has been developed in this protocol to increase its efficiency. Reductions in the level of confidentiality allow us to reduce the time spent on the execution of the authentication protocol. This increases the volume of information traffic. At the same time, an increase in the confidentiality of the protocol entails an increase in the time needed for authentication of the prover, which reduces the volume of information traffic. The FPGA Artix-7 xc7a12ticsg325-1L was used to estimate the time spent implementing the adaptive ZKAP protocol. Testing was performed for 32- and 64-bit adaptive authentication protocols.
Digital Mapping of Soil Profile Properties for Precision Agriculture in Developing Countries
Authors: López-Castañeda Antonio , Zavala-Cruz Joel , Palma-López David Jesús , Rincón-Ramírez Joaquín Alberto , Bautista Francisco
Published: January 2022

The soil profile and its spatial distribution are two essential aspects for promoting sustainable agriculture, with precise inputs in quantity, space, and time. This work’s objective was to elaborate a digital map of soil fertility considering the complete profile for the accurate management of amendments and fertilizers. For the preparation of the soil fertility map, the following inputs were used: a digital elevation model, information from 44 soil profiles, the conversion of the properties of the soil profiles into surface units, geostatistical analysis of the soil properties, and the preparation of the final map with a geographic information system. The best spatial models were achieved with CEC, pH, Ca, Mg, Na, and K. The map of the soil fertility classes was produced considering CEC and the pH value. The soil fertility classes presented the following sequence of occupied surfaces: very low, medium, very high, high, and low. A process was generated to elaborate digital maps (geostatistics) of soil fertility using taxonomic information from soil profiles and considering the complete profile. The process converts soil classification into geographic and soil fertility information from basic science to application.
Construction of a Humanized Artificial VHH Library Reproducing Structural Features of Camelid VHHs for Therapeutics
Authors: Murakami Taihei , Kumachi Shigefumi , Matsunaga Yasuhiro , Sato Miwa , Wakabayashi-Nakao Kanako , Masaki Hidekazu , Yonehara Ryo , Motohashi Maiko , Nemoto Naoto , Tsuchiya Masayuki
Published: January 2022

A variable domain of heavy chain antibody (VHH) has different binding properties than conventional antibodies. Conventional antibodies prefer binding to the convex portion of the antigen, whereas VHHs prefer epitopes, such as crevices and clefts on the antigen. Therefore, developing candidates with the binding characteristics of camelid VHHs is important. Thus, To this end, a synthetic VHH library that reproduces the structural properties of camelid VHHs was constructed. First, the characteristics of VHHs were classified according to the paratope formation based on crystal structure analyses of the complex structures of VHHs and antigens. Then, we classified 330 complementarity-determining region 3 (CDR3) structures of VHHs from the Protein Data Bank (PDB) into three loop structures: Upright, Half-Roll, and Roll. Moreover, these structures depended on the number of amino acid residues within CDR3. Furthermore, in the Upright loops, several amino acid residues in the FR2 are involved in the paratope formation, along with CDR3, suggesting that the FR2 design in the synthetic library is important. A humanized synthetic VHH library, comprising two sub-libraries, Upright and Roll, was constructed and named PharmaLogical. A validation study confirmed that our PharmaLogical library reproduces VHHs with the characteristics of the paratope formation of the camelid VHHs, and shows good performance in VHH screening.
Antibiotic Therapy in the Treatment of COVID-19 Pneumonia: Who and When?
Authors: Ng Tat Ming , Ong Sean W. X. , Loo Audrey Y. X. , Tan Sock Hoon , Tay Hui Lin , Yap Min Yi , Lye David C. , Lee Tau Hong , Young Barnaby E.
Published: January 2022

Background: COVID-19 imposes challenges in antibiotic decision-making due to similarities between bacterial pneumonia and moderate to severe COVID-19. We evaluated the effects of antibiotic therapy on the clinical outcomes of COVID-19 pneumonia patients and diagnostic accuracy of key inflammatory markers to inform antibiotic decision-making. Methods: An observational cohort study was conducted in patients hospitalised with COVID-19 at the National Centre for Infectious Diseases and Tan Tock Seng Hospital, Singapore, from January to April 2020. Patients were defined as receiving empiric antibiotic treatment for COVID-19 if started within 3 days of diagnosis. Results: Of 717 patients included, 86 (12.0%) were treated with antibiotics and 26 (3.6%) had documented bacterial infections. Among 278 patients with COVID-19 pneumonia, those treated with antibiotics had more diarrhoea (26, 34.7% vs. 24, 11.8%, p < 0.01), while subsequent admissions to the intensive care unit were not lower (6, 8.0% vs. 10, 4.9% p = 0.384). Antibiotic treatment was not independently associated with lower 30-day (adjusted odds ratio, aOR 19.528, 95% confidence interval, CI 1.039–367.021) or in-hospital mortality (aOR 3.870, 95% CI 0.433–34.625) rates after adjusting for age, co-morbidities and severity of COVID-19 illness. Compared to white cell count and procalcitonin level, the C-reactive protein level had the best diagnostic accuracy for documented bacterial infections (area under the curve, AUC of 0.822). However, the sensitivity and specificity were less than 90%. Conclusion: Empiric antibiotic use in those presenting with COVID-19 pneumonia did not prevent deterioration or mortality. More studies are needed to evaluate strategies to diagnose bacterial co-infections in these patients.
Exploiting Interdata Relationships in Prostate Cancer Proteomes: Clinical Significance of HO-1 Interactors
Authors: Lage-Vickers Sofia , Sanchis Pablo , Bizzotto Juan , Toro Ayelen , Sabater Agustina , Lavignolle Rosario , Anselmino Nicolas , Labanca Estefania , Paez Alejandra , Navone Nora , Valacco Maria P. , Cotignola Javier , Vazquez Elba , Gueron Geraldine
Published: January 2022

Prostate cancer (PCa) cells display abnormal expression of proteins resulting in an augmented capacity to resist chemotherapy and colonize distant organs. We have previously shown the anti-tumoral role of heme oxygenase 1 (HO-1) in this disease. In this work, we undertook a mass spectrometry-based proteomics study to identify HO-1 molecular interactors that might collaborate with its modulatory function in PCa. Among the HO-1 interactors, we identified proteins with nuclear localization. Correlation analyses, using the PCa GSE70770 dataset, showed a significant and positive correlation between HMOX1 and 6 of those genes. Alternatively, HMOX1 and YWHAZ showed a negative correlation. Univariable analyses evidenced that high expression of HNRNPA2B1, HSPB1, NPM1, DDB1, HMGA1, ZC3HAV1, and HMOX1 was associated with increased relapse-free survival (RFS) in PCa patients. Further, PCa patients with high HSPB1/HMOX1, DDB1/HMOX1, and YWHAZ/HMOX1 showed a worse RFS compared with patients with lower ratios. Moreover, a decrease in RFS for patients with higher scores of this signature was observed using a prognostic risk score model. However, the only factor significantly associated with a higher risk of relapse was high YWHAZ. Multivariable analyses confirmed HSPB1, DDB1, and YWHAZ independence from PCa clinic-pathological parameters. In parallel, co-immunoprecipitation analysis in PCa cells ascertained HO-1/14-3-3ζ/δ (protein encoded by YWHAZ) interaction. Herein, we describe a novel protein interaction between HO-1 and 14-3-3ζ/δ in PCa and highlight these factors as potential therapeutic targets.
1′-Acetoxyeugenol Acetate Isolated from Thai Ginger Induces Apoptosis in Human Ovarian Cancer Cells by ROS Production via NADPH Oxidase
Authors: Choi Ju-Yeon , Lee Na-Kyung , Wang Yi-Yue , Hong Joon-Pyo , Son So Ri , Gu Da-Hye , Jang Dae Sik , Choi Jung-Hye
Published: January 2022

The rhizomes of Alpinia galanga (Thai ginger) have been used extensively as a spice in Southeast Asian and Arabian cuisines and reported to possess a wide range of biological properties, such as antioxidant, antimicrobial, and antibacterial. However, the specific molecular and cellular mechanisms underlying the anti-tumor effects induced by Thai ginger and its corresponding active compounds have been poorly characterized. We found that upon EtOH extraction, Thai ginger extract exhibits cytotoxic activity (IC50 < 10 μg/mL) and triggers cell death via caspase-dependent apoptosis in human ovarian cancer cells. Among the three major compounds isolated from the extract, 1′-acetoxyeugenol acetate (AEA) exhibited potent cytotoxic activity in human ovarian cancer cells, SKOV3 and A2780. AEA induced apoptotic cell death through the activation of caspases-3 and -9. Notably, AEA enhanced the intracellular levels of reactive oxygen species (ROS), and the application of an antioxidant markedly reversed AEA-induced apoptosis of ovarian cancer cells. The knockdown of p47phox, a subunit of NADPH oxidase, suppressed both the pro-apoptotic and ROS-inducing effects of AEA. Additionally, the activation of the mitogen-activated protein kinase (MAPK) pathway by AEA through ROS regulation was found to be involved in AEA-induced apoptosis. Altogether, these results suggest that AEA exhibits potent apoptosis-inducing activity through the activation of the intrinsic pathway via ROS-mediated MAPK signaling in human ovarian cancer cells.
New Use of BIM-Origami-Based Techniques for Energy Optimisation of Buildings
Authors: Pavón Rubén Muñoz , Bazán Ángela Moreno , Cepa Jorge Jerez , Arcos Álvarez Antonio A. , Trigueros Jesús María Alonso , Alberti Marcos García , Tellaeche Jaime Reinoso
Published: January 2022

Outstanding properties and advanced functionalities of thermal–regulatory by origami-based architecture materials have been shown at various scales. However, in order to model and manage its programmable mechanical properties by Building Information Modelling (BIM) for use in a covering structure is not a simple task. The aim of this study was to model an element that forms a dynamic shell that prevents or allows the perpendicular incidence of the sun into the infrastructure. Parametric modelling of such complex structures was performed by Grasshopper and Rhinoceros 3D and were rendered by using the V-ray’s plugin. The elements followed the principles of origami to readjust its geometry considering the sun position, changing the shadow in real time depending on the momentary interest. The results of the project show that quadrangular was the most suitable Origami shape for façade elements. In addition, a BIM-based automated system capable of modifying façade elements considering the sun position was performed. The significance of this research relies on the first implementation and design of an Origami constructive element using BIM methodology, showing its viability and opening outstanding future research lines in terms of sustainability and energy efficiency.
An Improved Analytical Approach for Segmental Joint Rotational Behavior Considering the Elastic Gasket
Authors: Huang Ming , Yang Fan
Published: January 2022

To fully consider the effect of elastic gasket (EG) on the segmental joint rotational behavior, an improved analytical approach is proposed to simulate the joint rotations and obtain the rotational stiffnesses. By comparing the analytical results with the experimental results and numerical results, the joint compression zone should be simulated by the concrete–EG–concrete (CGC) model instead of rigid–EG–rigid (RGR) model or concrete–concrete (CC) model. Since the analytical approach with the CGC model can simulate the combined work of EG and concrete well, it is a more effective approach to obtain the joint rotational stiffnesses. According to these analytical results, the EG has a great impact on softening the joint rotations, and this leads the rotational stiffnesses of the joint with EG to be evidently smaller than those of the joint without EG. In addition, with decreasing the axial force or increasing the EG thickness, this softening effect tends to be more obvious. Therefore, the EG is a significant factor which should be fully considered during the theoretical analysis for the joint rotation behavior, or it may lead to an unreliable design for the segmental lining.
Insights of Raceway Bioreactor Scale-Up: Effect of Agitation on Microalgae Culture and Reduction of the Liquid Medium Speed
Authors: Bautista-Monroy Suri Sadai , Chávez-Urbiola Edgar Arturo , Ortega-Palacios Rocío , González-Sánchez Armando , Gómez-Aldapa Carlos Alberto , Rodríguez-Nava Odín , Salgado-Ramírez Julio Cesar , Cadena-Ramírez Arturo
Published: January 2022

The scaling of Raceway bioreactors was studied by geometric and mechanical similarity, with an order of magnitude of 1:10. The hydrodynamic parameters involved (flow velocity, hydrodynamic stress or shear stress, dimensionless numbers of Re, Fr, and Euler Power) at different stirring speeds (30, 35, 40, and 45 rpm) were determined. The study, carried out using low-density particle imaging velocimetry (PIV), showed that the speed of the liquid medium remains above 30 cm/s from 30 rpm, which ensures turbulence in the system. The flow velocity suffers a decrease of approximately 18%, at different angular velocities, with similar biomass concentrations (3.24 × 105–3.72 × 105 cells/mL). This decrease in speed directly affects the values of all the parameters involved in the bioreactor. Furthermore, the measurement of hydrodynamic stress (τ) indicates that the microorganisms are exposed to a value of 0.299 Pa at 35 rpm and 0.370 Pa at 40 rpm. Due to mechanical agitation, hydrodynamic stress values in Raceway systems have not been previously reported. The studies were carried out in a 10 L Raceway bioreactor using a consortium of microalgae and cyanobacteria where Spirulina sp. and Pseudanabaena sp. predominate.
The Choice of Optical Flame Detectors for Automatic Explosion Containment Systems Based on the Results of Explosion Radiation Analysis of Methane- and Dust-Air Mixtures
Authors: Khokhlov Sergey , Abiev Zaur , Makkoev Viacheslav
Published: January 2022

A review of the existing optoelectron monitoring devices revealed that the design of optoelectron detectors of the mine atmosphere does not sufficiently take into account the factor of external optical interference. This includes any extraneous source of thermal emission: a source of artificial lighting or enterprises. As a consequence, the optoelectron detectors -based safety systems currently installed at mining sites are not able to ensure properly the detection of the ignition source in the presence of optical interference. Thus, it is necessary to determine the working spectral wavelength ranges from methane and coal dust explosions. The article presents the results of experimental research devoted to the methane-air mixture and coal dust explosion spectral analysis by means of the photoelectric method. The ignition of a methane-air mixture of stoichiometric concentration (9.5%) and coal dust of size characterized by the dispersion of 63–94 microns and concentration of 200 g/m3 was carried out in a 20 L spherical chamber with an initial temperature in the setup of 18–22 °C at atmospheric pressure. Then, photometry of the explosion light flux was conducted on a photoelectric unit. Operating spectral wavelength ranges from methane and coal dust explosions were determined. For the methane-air mixture, it is advisable to use the spectral regions at the maximum emission of 390 and 900 nm. The spectrum section at the maximum emission of 620 nm was sufficient for dust-air mixture. It enabled us to select the wavelength ranges for automatic explosion suppression systems’ launching references. This will exclude false triggering of the explosion suppression system from other radiation sources. The research results will help to improve the decision-making credibility of the device in its direct design. The results will be used in further research to design noise-resistant optical flame detection sensors with a high response rate.
Effect of Excavation Blasting in the Arch Cover Method on Adjacent Existing Pipelines in a Subway Station
Authors: Liu Xiang , Jiang Annan , Guo Xinping , Hai Lu
Published: January 2022

The dynamic responses of existing pipelines are of great significance to be studied in blasting excavation in the arch cover method. In this paper, the Shi Kui Subway Station Line 5 in Dalian, China, is selected as a case study. A 3D numerical simulation model is established to analyze the dynamics characteristics of the existing pipelines subjected to blasting vibration, with a triangular pattern describing the blast hole pressure. The numerical simulation is verified by comparing the existing pipelines’ PPVs of the numerical model and field monitored points. Then, the dynamic responses of the pilot tunnels, existing pipelines, and secondary lining are discussed. The effects of the later pilot tunnel on the earlier pilot tunnel are remarkable when the relative distance between them is small. Extensive blasting areas and many charges will result in large peak velocities of the existing pipelines in a short time, as well as a decreased distance from the pilot tunnels. However, the hollow effect can change these dynamic characteristics. The implementation of a secondary lining can reduce the existing pipelines’ dynamic responses when the lower rock is blasted. The most adverse position of the secondary lining is the arch foot rather than the arch crown and arch waist; thus, blasting should be carried out at a suitable age for the concrete to ensure the safety of the structures and pipelines.
An Entity Relation Extraction Method Based on Dynamic Context and Multi-Feature Fusion
Authors: Ma Xiaolin , Wu Kaiqi , Kuang Hailan , Liu Xinhua
Published: January 2022

Dynamic context selector, a kind of mask idea, will divide the matrix into some regions, selecting the information of region as the input of model dynamically. There is a novel thought that improvement is made on the entity relation extraction (ERE) by applying the dynamic context to the training. In reality, most existing models of joint extraction of entity and relation are based on static context, which always suffers from the feature missing issue, resulting in poor performance. To address the problem, we propose a span-based joint extraction method based on dynamic context and multi-feature fusion (SPERT-DC). The context area is picked dynamically with the help of threshold in feature selecting layer of the model. It is noted that we also use Bi-LSTM_ATT to improve compatibility of longer text in feature extracting layer and enhance context information by combining with the tags of entity in feature fusion layer. Furthermore, the model in this paper outperforms prior work by up to 1% F1 score on the public dataset, which has verified the efficiency of dynamic context on ERE model.
Identification of Microorganisms Dwelling on the 19th Century Lanna Mural Paintings from Northern Thailand Using Culture-Dependent and -Independent Approaches
Authors: Suphaphimol Nattaphon , Suwannarach Nakarin , Purahong Witoon , Jaikang Churdsak , Pengpat Kamonpan , Semakul Natthawat , Yimklan Saranphong , Jongjitngam Surachai , Jindasu Saiklang , Thiangtham Sathaporn , Chantawannakul Panuwan , Disayathanoowat Terd
Published: January 2022

Lanna painting is a unique type of painting in many temples in the Northern Thai region. Similar to most mural paintings, they usually decay over time partly due to the activity of microbes. This study aimed to investigate the microorganisms from two Lanna masterpiece paintings in two temples that differ in the numbers of visitors using both culture-dependent and -independent approaches. The microorganisms isolated from the murals were also tested for the biodeterioration activities including discoloration, acid production and calcium precipitation. Most microorganisms extracted from the paintings were able to discolor the paints, but only fungi were able to discolor, produce acids and precipitate calcium. The microorganism communities, diversity and functional prediction were also investigated using the culture-independent method. The diversity of microorganisms and functional prediction were different between the two temples. Gammaproteobacteria was the predominant group of bacteria in both temples. However, the fungal communities were different between the two temples as Aspergillus was the most abundant genus in the site with higher number of visitors [Buak Krok Luang temple (BK)]. Conversely, mural paintings at Tha Kham temple (TK) were dominated by the Neodevriesia genera. We noticed that a high number of visitors (Buak Krok Luang) was correlated with microbial contamination from humans while the microbial community at Tha Kham temple had a higher proportion of saprotrophs. These results could be applied to formulate a strategy to mitigate the amount of tourists as well as manage microorganism to slow down the biodeterioration process.
Mitochondrial Transplantation Enhances Phagocytic Function and Decreases Lipid Accumulation in Foam Cell Macrophages
Authors: Játiva Soraya , Calle Priscila , Torrico Selene , Muñoz Ángeles , García Miriam , Martinez Ivet , Sola Anna , Hotter Georgina
Published: January 2022

Macrophages have mechanisms for eliminating cholesterol from cells. If excess cholesterol is not eliminated from the macrophages, then transformation into a foam cell may occur. Foam cells are a hallmark of the atherosclerotic lesions that contribute to the development and rupture of atherosclerotic plaques. Several in vitro and in vivo studies have shown changes in the macrophage phenotype and improved phagocytosis after the acquisition of functional mitochondria. However, the effect of mitochondrial transplantation on promoting phagocytosis and phenotypic changes in lipid-loaded macrophages leading to foam cells has not been studied. We aimed to prove that the transplantation of healthy mitochondria to highly cholesterol-loaded macrophages induces macrophage phagocytosis and reduces the macrophage shift towards foam cells. For this purpose, using a murine macrophage cell line, RAW264.7, we determined if mitochondria transplantation to 7-ketocholesterol (7-KC)-loaded macrophages reduced lipid accumulation and modified their phagocytic function. We evidenced that mitochondrial transplantation to 7-KC-loaded macrophages reestablished phagocytosis and reduced lipid content. In addition, CPT1a expression and anti-inflammatory cytokines were restored after mitochondrial transplantation. We have developed a potential therapeutic approach to restore foam cell functionality.