bluuebunny/crossref_metadata_2025 huggingface dataset:
Atrial Remodeling and the Substrate for Atrial Fibrillation in Rat Hearts With Elevated Afterload
Authors: Kim Shang-Jin , Choisy Stéphanie C.M. , Barman Palash , Zhang Haifei , Hancox Jules C. , Jones Sandra A. , James Andrew F.
Published: August 2011

Background—

Although arterial hypertension and left ventricular hypertrophy are considered good epidemiological indicators of the risk of atrial fibrillation (AF) in patients, the link between elevated afterload and AF remains unclear. We investigated atrial remodeling and the substrate for arrhythmia in a surgical model of elevated afterload in rats.

Methods and Results—

Male Wistar rats (aged 3–4 weeks) were anesthetized and subjected to either partial stenosis of the ascending aorta (AoB) or sham operation (Sham). Experiments were performed on excised hearts 8, 14, and 20 weeks after surgery. Unipolar electrograms were recorded from the left atrial epicardial surface of perfused hearts using a 5×5 electrode array. Cryosections of left atrial tissue were retained for histological and immunocytochemical analyses. Compared to Sham, AoB hearts showed marked left atrial hypertrophy and fibrosis at 14 and 20 weeks postsurgery. The incidence and duration of pacing-induced AF was increased in hearts from AoB rats at 20 weeks postsurgery. The substrate for arrhythmia was associated with reduced vectorial conduction velocity and greater inhomogeneity in conduction but without changes in effective refractory period. Left atrial expression of the gap junction protein, connexin43, was markedly reduced in AoB compared with Sham hearts.

Conclusions—

Using a small-animal model, we demonstrate that elevated afterload in the absence of systemic hypertension results in increased inducibility of AF and left atrial remodeling involving fibrosis, altered atrial connexin43 expression, and marked conduction abnormalities.
Accuracy and Prognostic Value of American Heart Association–Recommended Depression Screening in Patients With Coronary Heart Disease
Authors: Elderon Larkin , Smolderen Kim G. , Na Beeya , Whooley Mary A.
Published: August 2011

Background—

In 2008, the American Heart Association (AHA) recommended a 2-step screening method, consisting of the 2-item Patient Health Questionnaire (PHQ-2) followed by the 9-item Patient Health Questionnaire (PHQ-9), for identifying depression in cardiovascular patients. The accuracy and prognostic value of this screening method have not been evaluated.

Methods and Results—

We administered the 2-step AHA-recommended screening algorithm to 1024 patients with stable coronary heart disease and calculated sensitivity and specificity against a gold standard interview for major depressive disorder. Subsequent cardiovascular events (myocardial infarction, stroke, transient ischemic attack, heart failure, or death) were determined during a mean of 6.27±2.11 years of follow-up. The AHA-recommended screening method had high specificity (0.91; 95% confidence interval, 0.89 to 0.93) but low sensitivity (0.52; 95% confidence interval, 0.46 to 0.59) for a diagnosis of major depressive disorder. Participants who screened positive on the AHA depression protocol had a 55% greater risk of events than those who screened negative (age-adjusted hazard ratio, 1.55; 95% confidence interval, 1.21 to 1.97;
P
=0.0005). After adjustment for age, sex, body mass index, history of myocardial infarction, hypertension, diabetes, heart failure, and high-density lipoprotein levels, screening positive remained associated with a 41% greater rate of cardiovascular events (hazard ratio, 1.41; 95% confidence interval, 1.10 to 1.81;
P
=0.008).

Conclusions—

Among outpatients with stable coronary heart disease, the AHA-recommended depression screening protocol is highly specific for depression and identifies patients at risk for adverse cardiovascular outcomes.
Depth Perception Within Peripersonal Space Using Head-Mounted Display
Authors: Naceri Abdeldjallil , Chellali Ryad , Hoinville Thierry
Published: August 2011

In this paper, we address depth perception in the peripersonal space within three virtual environments: poor environment (dark room), reduced cues environment (wireframe room), and rich cues environment (a lit textured room). Observers binocularly viewed virtual scenes through a head-mounted display and evaluated the egocentric distance to spheres using visually open-loop pointing tasks. We conducted two different experiments within all three virtual environments. The apparent size of the sphere was held constant in the first experiment and covaried with distance in the second one. The results of the first experiment revealed that observers more accurately estimated depth in the rich virtual environment compared to the visually poor and the wireframe environments. Specifically, observers' pointing errors were small in distances up to 55 cm, and increased with distance once the sphere was further than 55 cm. Individual differences were found in the second experiment. Our results suggest that the quality of virtual environments has an impact on distance estimation within reaching space. Also, manipulating the targets' size cue led to individual differences in depth judgments. Finally, our findings confirm the use of vergence as an absolute distance cue in virtual environments within the arm's reaching space.
Characterization of Aircraft Icing Environments with Supercooled Large Drops for Application to Commercial Aircraft Certification
Authors: Cober Stewart G. , Isaac George A.
Published: August 2011

Observations of aircraft icing environments that included supercooled large drops (SLD) greater than 100 μm in diameter have been analyzed. The observations were collected by instrumented research aircraft from 134 flights during six field programs in three different geographic regions of North America. The research aircraft were specifically instrumented to accurately measure the microphysics characteristics of SLD conditions. In total 2444 SLD icing environments were observed at 3-km resolution. Each observation had an average liquid water content (LWC) > 0.005 g m−3, drops > 100 μm in diameter, ice crystal concentrations <1 L−1, and an average static temperature ≤0°C. SLD conditions were observed approximately 5% of the in-flight time. The SLD observations were segregated into four subsets, which included conditions with maximum drop sizes <500 μm and >500 μm in diameter, each with median drop volume diameters <40 μm and >40 μm. For each SLD subset, the observations were used to develop envelopes of maximum LWC values as a function of horizontal extent and temperature. In addition, characteristic drop size distributions were developed for each SLD subset. The maximum LWC values physically represent either the 99% or 99.9% LWC values, as determined from an extreme value analysis of the data. The analysis is sufficient for simulation of SLD environments with either numerical icing accretion models or wind-tunnel icing simulations. The SLD envelopes are similar in structure and supplemental to existing aircraft icing envelopes, the difference being that the existing envelopes did not explicitly incorporate SLD conditions.
Wind Speed and Stability Effects on Coupling between Surface Wind Stress and SST Observed from Buoys and Satellite
Authors: O’Neill Larry W.
Published: August 2011

The surface wind and stress responses to sea surface temperature (SST) are examined using collocated moored buoy and satellite observations in the Gulf Stream and the eastern equatorial Pacific. Using 17 buoy pairs, differences in the wind speed, 10-m equivalent neutral wind speed (ENW), and surface wind stress magnitude between two buoys separated by between 150 and 350 km were all found to be highly correlated to, and satisfy linear relations with, the SST difference on time scales longer than 10 days. This wind–SST coupling is consistent with previous analyses of spatially high-pass-filtered satellite ENW and SST fields. For all buoy pairs, the ENW and wind speed responses to SST differ by only 10%–30%, indicating that the ENW and stress responses to SST are attributable primarily to the response of the actual surface wind speed to SST rather than to stability. This result clarifies the dynamical pathway of the wind–SST coupling on the oceanic mesoscale.

This buoy-pair methodology is used further to evaluate the ENW–SST coupling derived from collocated satellite observations of ENW by the Quick Scatterometer (QuikSCAT) and SST by the Advanced Microwave Scanning Radiometer for Earth Observing System (AMSR-E) on board the Aqua satellite. Overall, the satellite and buoy ENW responses to SST compare well, with normalized mean differences (satellite minus buoy) of 17% over the Gulf Stream and −31% and 2% over the southern and northern sides of the equatorial Pacific, respectively.

Finally, seasonal variability of the large-scale ENW is shown to modulate the wind stress response to SST, whereby stronger winter wind enhances the stress response by a factor of ~2 relative to the ENW response.
Desecularization of the Palestinian Imagination? Reflections on Nation-Ness and Fragmentation
Authors: Hovsepian Mary
Published: August 2011

The main process that gives meaning to belonging to the Palestinian nation concerns the everyday encounters with Israeli policies that dictate Palestinian lives. The escalating fragmentation of the occupied territories since the mid-1990s and the control of the most basic right, the right of freedom of movement, continue to shape Palestinian nation-ness in the context of a struggle to end the Israeli occupation.
Does Enterprise Risk Management Increase Firm Value?
Authors: McShane Michael K. , Nair Anil , Rustambekov Elzotbek
Published: August 2011

Enterprise risk management (ERM) has emerged as a construct that ostensibly overcomes limitations of silo-based traditional risk management (TRM), yet little is known about its effectiveness. The scant research on the relationship between ERM and firm performance has offered mixed findings and has been limited by the lack of a suitable proxy for the degree of ERM implementation. Using Standard and Poor’s newly available risk management rating, the authors find evidence of a positive relationship between increasing levels of TRM capability and firm value but no additional increase in value for firms achieving a higher ERM rating. Considering these results, the authors suggest directions for future research.
The differentiation of international societies: An approach to structural international theory
Authors: Donnelly Jack
Published: August 2011

Taking off from a recent article in this journal by Barry Buzan and Mathias Albert, I present a framework of structural differentiation as an alternative to IR’s standard tripartite conception of the elements of structure. Rather than employ ideal type models, though, as Buzan and Albert do, I present a more open-textured, multidimensional account of differentiation. I also emphasize the systemic nature of structural analysis. The elements of structure are interdependent parts of wholes (systems) — not the independent variables implied by standard formulations such as ‘the effects of anarchy’. A multidimensional systemic approach directs our attention to the diversity of and change in international systems and their structures, which mainstream structural IR typically ignores or obscures. I illustrate both the regularity of extensive structural change and the analytical utility of my differentiation framework with case studies of post-World War II international society and contemporary processes of globalization.
The determinants of stroke phenotypes were different from the predictors (CHADS2 and CHA2DS2-VASc) of stroke in patients with atrial fibrillation: a comprehensive approach
Authors: Oh Semi , Kim Suk Jae , Ryu Soo-Kyoung , Kim Gyeong-Moon , Chung Chin Sang , Lee Kwang Ho , Bang Oh Young
Published: August 2011

Background

Atrial fibrillation (AF) is a leading cause of fatal ischemic stroke. It was recently reported that international normalized ratio (INR) levels were associated with infarct volumes. However, factors other than INR levels that affect stroke phenotypes are largely unknown. Therefore, we evaluated the determinants of stroke phenotypes (pattern and volume) among patients with AF who were not adequately anticoagulated.

Methods

We analyzed data pertaining to consecutive AF patients admitted over a 6-year period with acute MCA territory infarcts. We divided the patients according to DWI (diffusion-weighted imaging) lesion volumes and patterns, and the relationship between stroke predictors (the CHADS2 and CHA2DS2-VASc score), systemic, and local factors and each stroke phenotype were then evaluated.

Results

The stroke phenotypes varied among 231 patients (admission INR median 1.06, interquartile range (IQR) 1.00-1.14). Specifically, (1) the DWI lesion volumes ranged from 0.04-338.62 ml (median 11.86 ml; IQR, 3.07-44.20 ml) and (2) 46 patients had a territorial infarct pattern, 118 had a lobar/deep pattern and 67 had a small scattered pattern. Multivariate testing revealed that the CHADS2 and CHA2DS2-VASc score were not related to either stroke phenotype. Additionally, the prior use of antiplatelet agents was not related to the stroke phenotypes. Congestive heart failure and diastolic dysfunction were not associated with stroke phenotypes.

Conclusions

The results of this study indicated that the determinants of stroke phenotypes were different from the predictors (i.e., CHADS2 and CHA2DS2-VASc score) of stroke in patients with AF.
A successfully thrombolysed acute inferior myocardial infarction due to type A aortic dissection with lethal consequences: the importance of early cardiac echocardiography
Authors: Tsigkas Grigorios , Kasimis Georgios , Theodoropoulos Konstantinos , Chouchoulis Konstantinos , Baikoussis Nikolaos G , Apostolakis Efstratios , Bousoula Eleni , Moulias Athanasios , Alexopoulos Dimitrios
Published: August 2011

Thrombolysis, a standard therapy for ST elevation myocardial infarction (STEMI) in non-PCI-capable hospitals, may be catastrophic for patients with aortic dissection leading to further expansion, rupture and uncontrolled bleeding. Stanford type A aortic dissection, rarely may mimic myocardial infarction. We report a case of a patient with an inferior STEMI thrombolysed with tenecteplase and followed by clinical and electrocardiographic evidence of successful reperfusion, which was found later to be a lethal acute aortic dissection. Prognostic implications of early diagnosis applying transthoracic echocardiography (TTE) are described.
Unusual exanthema combined with cerebral vasculitis in pneumococcal meningitis: a case report
Authors: Tavladaki Theonimfi , Spanaki Anna-Maria , Ilia Stavroula , Geromarkaki Elisabeth , Raissaki Maria , Briassoulis George
Published: August 2011

Introduction

Bacterial meningitis is a complex, rapidly progressive disease in which neurological injury is caused in part by the causative organism and in part by the host's own inflammatory responses.

Case presentation

We present the case of a two-year-old Greek girl with pneumococcal meningitis and an atypical curvilinear-like skin eruption, chronologically associated with cerebral vasculitis. A diffusion-weighted MRI scan showed lesions with restricted diffusion, reflecting local areas of immunologically mediated necrotizing vasculitis.

Conclusions

Atypical presentations of bacterial meningitis may occur, and they can be accompanied by serious unexpected complications.
Recurrent takotsubo cardiomyopathy in the setting of transient neurological symptoms: a case report
Authors: Sardar Muhammad Rizwan , Kuntz Catherine , Mazurek Jeremy A , Akhtar Naveed Hassan , Saeed Wajeeha , Shapiro Timothy
Published: August 2011

Introduction

First described in Japan, takotsubo cardiomyopathy is increasingly becoming recognized worldwide as a cause of sudden and reversible diminished left ventricular function characterized by left apical ballooning and hyperkinesis of the basal segments, often with symptoms mimicking a myocardial infarction. Associated with physical or emotional stress, its exact pathogenesis has not been established, though evidence supports a neurohumoral etiology. Additionally, recurrence of this condition is rare. In this report, we present a rare case of recurrent takotsubo cardiomyopathy in a post-menopausal woman who presented with transient neurological complaints on both occasions.

Case presentation

We present a rare case of a 76-year-old Caucasian woman with no history of congestive heart failure who presented to our emergency department twice with transient neurological complaints. On the first occasion, she was found to have transient aphasia which resolved within 24 hours, yet during that period she also developed symptoms of congestive heart failure and was noted to have a new, significantly depressed ejection fraction with apical akinesis and possible apical thrombus. One month after her presentation a repeat echocardiogram revealed complete resolution of all wall motion abnormalities and a return to baseline status. Seven months later she presented with ataxia, was diagnosed with vertebrobasilar insufficiency, and again developed symptoms and echocardiography findings similar to those of her first presentation. Once again, at her one-month follow-up examination, all wall motion abnormalities had completely resolved and her ejection fraction had returned to normal.

Conclusion

Though the exact etiology of takotsubo cardiomyopathy is unclear, a neurohumoral mechanism has been proposed. Recurrence of this disorder is rare, though it has been reported in patients with structural brain abnormalities. This report is the first to describe recurrent takotsubo cardiomyopathy in a patient with transient neurological symptoms. In our patient, as expected in patients with this condition, complete resolution of all left ventricular abnormalities occurred within a short period of time. It is important for clinicians to be aware of this increasingly recognized syndrome, including its association with recurrence, especially in the clinical setting of neurologic dysfunction.
Maternal Obesity Promotes a Proinflammatory Signature in Rat Uterus and Blastocyst
Authors: Shankar Kartik , Zhong Ying , Kang Ping , Lau Franchesca , Blackburn Michael L. , Chen Jin-Ran , Borengasser Sarah J. , Ronis Martin J. J. , Badger Thomas M.
Published: August 2011

Maternal obesity at conception increases the risk of offspring obesity, thus propagating an intergenerational vicious cycle. Male offspring born to obese dams are hyperresponsive to high fat-diets, gaining greater body weight, fat mass, and additional metabolic sequelae compared to lean controls. In this report, we identify the impact of maternal obesity before conception, on the embryo, and intrauterine milieu during the periimplantation period. We conducted global transcriptomic profiling in the uterus and periimplantation blastocyst, gene/protein expression analyses of inflammatory pathways in conjunction with endocrine and metabolic characterization in the dams at implantation. Uterine gene expression profiles of lean and obese dams revealed distinct signatures for genes regulating inflammation and lipid metabolism. Both pathway and gene-set enrichment analysis revealed uterine nuclear factor-κB and c-Jun N-terminal kinase signaling to be up-regulated in the uterus of obese dams, which was confirmed via immunoblotting. Obese uteri also evidenced an inflammatory secretome with higher chemokine mRNA abundance (CCL2, CCL5, CCL7, and CxCL10) and related regulators (TLR2, CD14, and Ccr1). Increased inflammation in the uterus was associated with ectopic lipid accumulation and expression of lipid metabolic genes. Gene expression in sex-identified male periimplantation blastocyst at day postcoitum 4.5 was clearly influenced by maternal obesity (359 transcripts, ±1.4-fold), including changes in developmental and epigenetic regulators. Akin to the uterus, nuclear factor-κB-regulated proinflammatory genes (CCL4 and CCL5) increased and expression of antioxidant (GPx3) and mitochondrial (TFAM and NRF1) genes decreased in the obese embryos. Our results suggest that ectopic lipid and inflammation may link maternal obesity to increased predisposition of offspring to obesity later in life.
Induction of Autoimmune Thyroiditis by Depletion of CD4+CD25+ Regulatory T Cells in Thyroiditis-Resistant IL-17, But Not Interferon-γ Receptor, Knockout Nonobese Diabetic-H2h4 Mice
Authors: Horie Ichiro , Abiru Norio , Sakamoto Hikaru , Iwakura Yoichiro , Nagayama Yuji
Published: August 2011

Iodine-induced experimental autoimmune thyroiditis in the nonobese diabetic (NOD)-H2h4 mouse is a prototype of animal models of Hashimoto's thyroiditis in humans. Recent studies have shown the resistance to thyroiditis of NOD-H2h4 mice genetically deficient for either IL-17 or interferon (IFN)-γ, implicating both of T helper type 1 (Th1) and Th17 immune responses in disease pathogenesis. However, we hypothesized that robust induction of a single arm of effector T cells (either Th1 or Th17) might be sufficient for inducing thyroiditis in NOD-H2h4 mice. To address this hypothesis, enhanced immune responses consisting of either Th1 or Th17 were induced by anti-CD25 antibody-mediated depletion of regulatory T cells (Treg) in thyroiditis-resistant IL-17 knockout (KO) or IFN-γ receptor (IFN-γR) KO, respectively, NOD-H2h4 mice. Depletion of Treg in IL-17 KO mice (i.e. Th1 enhancement) elicited antithyroglobulin autoantibodies and thyroiditis. Immunohistochemical analysis of the thyroid glands revealed the similar intrathyroidal lymphocyte infiltration patterns, with CD4+ T and CD19+ B cells being dominant between the wild-type and Treg-depleted IL-17 KO mice. In contrast, Treg-depleted IFN-γR KO mice remained thyroiditis resistant. Intracellular cytokine staining assays showed differentiation of Th1 cells in IL-17 KO mice but not of Th17 cells in IFN-γR KO mice. Our findings demonstrate that a robust Th1 immune response can by itself induce thyroiditis in otherwise thyroiditis-resistant IL-17 KO mice. Thus, unlike Th17 cells in IFN-γR KO mice, Th1 cells enhanced by Treg depletion can be sustained and induce thyroiditis.
Cold-impaired cardiac performance in rats is only partially overcome by cold acclimation
Authors: Hauton David , May Shaun , Sabharwal Rasna , Deveci Durmus , Egginton Stuart
Published: August 2011

The consequences of acute hypothermia include impaired cardiovascular performance, ultimately leading to circulatory collapse. We examined the extent to which this results from intrinsic limitations to cardiac performance or physiological dysregulation/autonomic imbalance, and whether chronic cold exposure could ameliorate the impaired function. Wistar rats were held at a 12 h:12 h light:dark (L:D) photoperiod and room temperature (21°C; euthermic controls), or exposed to a simulated onset of winter in an environmental chamber by progressive acclimation to 1 h:23 h L:D and 4°C over 4 weeks. In vivo, acute cold exposure (core temperature, Tb=25°C) resulted in hypotension (approximately –20%) due to low cardiac output (approximately –30%) accompanying a bradycardia (approximately –50%). Cold acclimation (CA) induced only partial compensation for this challenge, including increased coronary flow at Tb=37°C (but not at Tb=25°C), maintenance of ventricular capillarity and altered sympathovagal balance (increased low:high frequency in power spectral analysis, PSA), suggesting physiological responses alone were insufficient to maintain cardiovascular performance. However, PSA showed maintenance of cardiorespiratory coupling on acute cold exposure in both groups. Ex vivo cardiac performance revealed no change in intrinsic heart rate, but a mechanical impairment of cardiac function at low temperatures following CA. While CA involved an increased capacity for β-oxidation, there was a paradoxical reduction in developed pressure as a result of adrenergic down-regulation. These data suggest that integrated plasticity is the key to cardiovascular accommodation of chronic exposure to a cold environment, but with the potential for improvement by intervention, for example with agents such as non-catecholamine inotropes.
Movement and function of the pectoral fins of the larval zebrafish (Danio rerio) during slow swimming
Authors: Green Matthew H. , Ho Robert K. , Hale Melina E.
Published: August 2011

Pectoral fins are known to play important roles in swimming for many adult fish; however, their functions in fish larvae are unclear. We examined routine pectoral fin movement during rhythmic forward swimming and used genetic ablation to test hypotheses of fin function in larval zebrafish. Fins were active throughout bouts of slow swimming. Initiation was characterized by asymmetric fin abduction that transitioned to alternating rhythmic movement with first fin adduction. During subsequent swimming, fin beat amplitude decreased while tail beat amplitude increased over swimming speeds ranging from 1.47 to 4.56 body lengths per second. There was no change in fin or tail beat frequency with speed (means ± s.d.: 28.2±3.5 and 29.6±1.9 Hz, respectively). To examine potential roles of the pectoral fins in swimming, we compared the kinematics of finless larvae generated with a morpholino knockdown of the gene fgf24 to those of normal fish. Pectoral fins were not required for initiation nor did they significantly impact forward rhythmic swimming. We investigated an alternative hypothesis that the fins function in respiration. Dye visualization demonstrated that pectoral fin beats bring distant fluid toward the body and move it caudally behind the fins, disrupting the boundary layer along the body's surface, a major site of oxygen absorption in larvae. Larval zebrafish also demonstrated more fin beating in low oxygen conditions. Our data reject the hypothesis that the pectoral fins of larval zebrafish have a locomotor function during slow, forward locomotion, but are consistent with the hypothesis that the fins have a respiratory function.
Magnetic orientation of migratory robins, Erithacus rubecula, under long-wavelength light
Authors: Wiltschko Roswitha , Denzau Susanne , Gehring Dennis , Thalau Peter , Wiltschko Wolfgang
Published: August 2011

The avian magnetic compass is an inclination compass that appears to be based on radical pair processes. It requires light from the short-wavelength range of the spectrum up to 565 nm green light; under longer wavelengths, birds are disoriented. When pre-exposed to longer wavelengths for 1 h, however, they show oriented behavior. This orientation is analyzed under 582 nm yellow light and 645 nm red light in the present study: while the birds in spring prefer northerly directions, they do not show southerly tendencies in autumn. Inversion of the vertical component does not have an effect whereas reversal of the horizontal component leads to a corresponding shift, indicating that a polar response to the magnetic field is involved. Oscillating magnetic fields in the MHz range do not affect the behavior but anesthesia of the upper beak causes disorientation. This indicates that the magnetic information is no longer provided by the radical pair mechanism in the eye but by the magnetite-based receptors in the skin of the beak. Exposure to long-wavelength light thus does not expand the spectral range in which the magnetic compass operates but instead causes a different mechanism to take over and control orientation.
Moufang loops that are almost groups
Authors: Chee Wing Loon , Rajah Andrew
Published: August 2011

It is known that Moufang loops are closely related to groups as they have many properties in common. For instance, they both have an inverse for each element and satisfy Lagrange's theorem. In this paper, we study the properties of a class of Moufang loops which are not groups, but all their proper subloops and proper quotient loops are groups.
The Representation of Visual and Motor Aspects of Reaching Movements in the Human Motor Cortex
Authors: Eisenberg Michal , Shmuelof Lior , Vaadia Eilon , Zohary Ehud
Published: August 2011

The human primary motor cortex (M1) is robustly activated during visually guided hand movements. M1 multivoxel patterns of functional MRI activation are more correlated during repeated hand movements to the same targets than to greatly differing ones, and therefore potentially contain information about movement direction. It is unclear, however, whether direction specificity is due to the motor command, as implicitly assumed, or to the visual aspects of the task, such as the target location and the direction of the cursor's trajectory. To disambiguate the visual and motor components, different visual-to-motor transformations were applied during an fMRI scan, in which participants made visually guided hand movements in various directions. The first run was the “baseline” (i.e., visual and motor mappings were matched); in the second run (“rotation”), the cursor movement was rotated by 45° with respect to the joystick movement. As expected, positive correlations were seen between the M1 multivoxel patterns evoked by the baseline run and by the rotation run, when the two movements were matched in their movement direction but the visual aspects differed. Importantly, similar correlations were observed when the visual elements were matched but the direction of hand movement differed. This indicates that M1 is sensitive to both motor and visual components of the task. However, repeated observation of the cursor movement without concurrent joystick control did not elicit significant activation in M1 or any correlated patterns of activation. Thus, visual aspects of movement are encoded in M1 only when they are coupled with motor consequences.
Two Amino Acid Residues Contribute to a Cation-π Binding Interaction in the Binding Site of an Insect GABA Receptor
Authors: Lummis Sarah C. R. , McGonigle Ian , Ashby Jamie A. , Dougherty Dennis A.
Published: August 2011

Cys-loop receptor binding sites characteristically possess an “aromatic box,” where several aromatic amino acid residues surround the bound ligand. A cation-π interaction between one of these residues and the natural agonist is common, although the residue type and location are not conserved. Even in the closely related vertebrate GABAAand GABACreceptors, residues in distinct locations perform this role: in GABAAreceptors, a Tyr residue in loop A forms a cation-π interaction with GABA, while in GABACreceptors it is a loop B residue. GABA-activated Cys-loop receptors also exist in invertebrates, where they have distinct pharmacologies and are the target of a range of pesticides. Here we examine the location of GABA in an insect binding site by incorporating a series of fluorinated Phe derivatives into the receptor binding pocket using unnatural amino acid mutagenesis, and evaluating the resulting receptors when expressed inXenopusoocytes. A homology model suggests that two aromatic residues (in loops B and C) are positioned such that they could contribute to a cation-π interaction with the primary ammonium of GABA, and the data reveal a clear correlation between the GABA EC50and the cation-π binding ability both at Phe206 (loop B) and Tyr254 (loop C), demonstrating for the first time the contribution of two aromatic residues to a cation-π interaction in a Cys-loop receptor.
Cyclin B1 Expression Regulated by Cytoplasmic Polyadenylation Element Binding Protein in Astrocytes
Authors: Kim Ki Chan , Oh Won Jung , Ko Kwang Ho , Shin Chan Young , Wells David G.
Published: August 2011

Astrocytes are the most abundant cells in the brain, playing vital roles in neuronal survival, growth, and function. Understanding the mechanism(s) regulating astrocyte proliferation will have important implications in brain development, response to injury, and tumorigenesis. Cyclin B1 is well known to be a critical regulator of mitotic entry via its interaction with cyclin-dependent kinase 1. In rat astrocytes, we now show that the mRNA binding protein cytoplasmic polyadenylation element binding protein 1 (CPEB1) is associated with cyclin B1 mRNA and that this interaction is enriched at the centrosome. In addition, if growth-arrested astrocytes are stimulated to divide, CPEB1 is phosphorylated and cyclin B1 mRNA is polyadenylated, both hallmarks of CPEB1 activation, resulting in an increase in cyclin B1 protein. CPEB1 binding to mRNA initially inhibits translation; therefore, removing CPEB1 from mRNA should result in an increase in translation due to derepression. Indeed, when we either knocked down CPEB1 protein with siRNA or sequestered it from endogenous mRNA by expressing RNA containing multiple CPEB1 binding sites, cyclin B1 protein was increased and cell proliferation was stimulated. Our data suggest a mechanism wherein CPEB1 is bound and represses cyclin B1 mRNA translation until a signal to proliferate phosphorylates CPEB1, resulting in an increase in cyclin B1 protein and progression into mitosis. Our results demonstrate for the first time a role for CPEB1 in regulating cell proliferation in the brain.
Leptin Action in the Dorsomedial Hypothalamus Increases Sympathetic Tone to Brown Adipose Tissue in Spite of Systemic Leptin Resistance
Authors: Enriori Pablo J. , Sinnayah Puspha , Simonds Stephanie E. , Garcia Rudaz Cecilia , Cowley Michael A.
Published: August 2011

Leptin regulates body weight in mice by decreasing appetite and increasing sympathetic nerve activity (SNA), which increases energy expenditure in interscapular brown adipose tissue (iBAT). Diet-induced obese mice (DIO) are resistant to the anorectic actions of leptin. We evaluated whether leptin still stimulated sympathetic outflow in DIO mice. We measured iBAT temperature as a marker of SNA. We found that obese hyperleptinemic mice have higher iBAT temperature than mice on regular diet. Conversely, obese leptin-deficientob/obmice have lower iBAT temperature. Additionally, leptin increased SNA in obese (DIO andob/ob) and control mice, despite DIO mice being resistant to anorectic action of leptin. We demonstrated that neurons in the dorsomedial hypothalamus (DMH) of DIO mice mediate the thermogenic responses to hyperleptinemia in obese mammals because blockade of leptin receptors in the DMH prevented the thermogenic effects of leptin.

Peripheral Melotan II (MTII) injection increased iBAT temperature, but it was blunted by blockade of DMH melanocortin receptors (MC4Rs) by injecting agouti-related peptide (AgRP) directly into the DMH, suggesting a physiological role of the DMH on temperature regulation in animals with normal body weight. Nevertheless, obese mice without a functional melanocortin system (MC4R KO mice) have an increased sympathetic outflow to iBAT compared with their littermates, suggesting that higher leptin levels drive sympathoexcitation to iBAT by a melanocortin-independent pathway.

Because the sympathetic nervous system contributes in regulating blood pressure, heart rate, and hepatic glucose production, selective leptin resistance may be a crucial mechanism linking adiposity and metabolic syndrome.
Methodologies to study the surface hydraulic behaviour of urban catchments during storm events
Authors: Gómez M. , Macchione F. , Russo B.
Published: August 2011

A good knowledge of the hydraulic behaviour of an urban catchment and its surface drainage system is an essential requirement to guarantee traffic and pedestrian safety. In many cases, inlets have been situated according to spatial density criteria. Indeed a more rational location of inlets on urban catchments must be defined according to an accurate analysis of the relationship between street flow and inlet hydraulic efficiency. Moreover we lack specific hazard criteria in terms of the maximum acceptable flow depths and velocities on the streets that do not cause problems to pedestrians. In this paper the results of two different experimental campaigns are presented. The first was carried out to evaluate inlet hydraulic efficiency; the second was carried out to address the pedestrian stability in urban flood conditions, whose aim was to propose new hazard criteria. On the basis of the experimental results, a methodology was developed to assess flood hazard in urban areas during storm events. If a refined topographic representation of urban areas is available, a two-dimensional numerical simulation of urban flooding can be performed using complete shallow water equations. According to this approach a numerical application for flood hazard assessment in a street of Barcelona is shown.
Experiences on dual media filtration of WWTP effluent
Authors: Scherrenberg S. M. , Postma P. , Neef R. , Menkveld H. W. H. , Bechger M. , van der Graaf J. H. J. M.
Published: August 2011

This research is legislation driven by the European Water Framework Directive (WFD) and the Dutch Fourth Memorandum on Water Management. The objective of this research is to achieve the removal of total nitrogen and total phosphorus by Dual Media Filtration. The target value during this research for total nitrogen is 2.2 mg/L and for total phosphorus 0.15 mg/L. The results show that for NOx-N concentrations in the WWTP effluent up to 10 mg/L, a stable operation of the process can be reached with removal rates of 80% to 90%. The maximum nitrogen removal rate was 3.5 kg N/(m3.d). Above 10 mg/L a risk of filter bed clogging occurred. When the orthophosphorus concentration in the WWTP effluent exceeds the maximum of 0.3 mg/L, the total phosphorus concentration in the filtrate water will exceed the target value of 0.15 mg P-total/L. Temperature has a large impact in the phosphorus removal; the optimum temperature range is within 13°C–18°C. In conclusion, Dual Media Filtration is capable of producing reusable water with total phosphorus concentrations of <0.15 mg/L, under the condition that the wastewater treatment plant produces WWTP effluent with steady concentrations for orthophosphorus (<0.3 mg PO4-P/L). To reach total nitrogen concentrations in the filtrate water of <2.2 mg/L a NOx-N removal efficiency of nearly 100% is required.
Identification of humic acid-like and fulvic acid-like natural organic matter in river water using fluorescence spectroscopy
Authors: Peiris R. H. , Budman H. , Moresoli C. , Legge R. L.
Published: August 2011

Identifying the extent of humic acid (HA)-like and fulvic acid (FA)-like natural organic matter (NOM) present in natural water is important to assess disinfection by-product formation and fouling potential during drinking water treatment applications. However, the unique fluorescence properties related to HA-like NOM is masked by the fluorescence signals of the more abundant FA-like NOM. For this reason, it is not possible to accurately characterize HA-like and FA-like NOM components in a single water sample using direct fluorescence EEM analysis. A relatively simple approach is described here that demonstrates the feasibility of using a fluorescence excitation-emission matrix (EEM) approach for identifying HA-like and FA-like NOM fractions in water when used in combination with a series of pH adjustments and filtration steps. It is demonstrated that the fluorescence EEMs of HA-like and FA-like NOM fractions from the river water sample possessed different spectral properties. Fractionation of HA-like and FA-like NOM prior to fluorescence analysis is therefore proposed as a more reasonable approach.
Prediction of scour below submerged pipeline crossing a river using ANN
Authors: Azamathulla H. Md. , Zakaria Nor Azazi
Published: August 2011

The process involved in the local scour below pipelines is so complex that it makes it difficult to establish a general empirical model to provide accurate estimation for scour. This paper describes the use of artificial neural networks (ANN) to estimate the pipeline scour depth. The data sets of laboratory measurements were collected from published works and used to train the network or evolve the program. The developed networks were validated by using the observations that were not involved in training. The performance of ANN was found to be more effective when compared with the results of regression equations in predicting the scour depth around pipelines.
On-site evaluation of the removal of 100 micro-pollutants through advanced wastewater treatment processes for reuse applications
Authors: Ruel S. Martin , Choubert J. M. , Esperanza M. , Miège C. , Navalón Madrigal P. , Budzinski H. , Le Ménach K. , Lazarova V. , Coquery M.
Published: August 2011

The next challenge of wastewater treatment is to reliably remove micro-pollutants at the microgram per litre range in order to meet reuse applications and contribute to reach the good status of the water bodies. A hundred priority and relevant emerging substances were measured to evaluate at full-scale the removal efficiencies of seven advanced treatment lines (one membrane bioreactor process and six tertiary treatment lines) that were designed for reuse applications. To reliably compare the processes, specific procedures for micro-pollutants were applied for sampling, analysis and calculation of removal efficiencies. The membrane bioreactor process allowed to upgrade the removal efficiencies of about 20% of the substances measured, especially those that were partially degraded during conventional processes. Conventional tertiary processes like high rate clarification, sand filtration and polishing pond achieved significant removal for some micro-pollutants, especially for adsorbable substances. Advanced tertiary processes, like ozonation, activated carbon and reverse osmosis were all very efficient to complete the removal of polar pesticides and pharmaceuticals; metals and less polar substances were better retained by reverse osmosis.
Respirometric assays of two different MBR (microfiltration and ultrafiltration) to obtain kinetic and stoichiometric parameters
Authors: Ruiz L. M. , Arévalo J. , Parada J. , González D. , Moreno B. , Pérez J. , Gómez M. A.
Published: August 2011

A comparison of two different medium scale MBRs (ultrafiltration and microfiltration) using respirometric methods has been achieved. The ultrafiltration membrane plant (0.034 µm pore size) maintained recirculation sludge flow at seven times the influent flow, and membranes were backwashed every 5 min and chemically cleaned weekly. The microfiltration membrane plant (0.4 µm pore size) maintained recirculation sludge flow at four times the influent flow, membrane-relax was applied after the production phase and membranes were chemically cleaned in the event of high trans-membrane pressure. Both technologies showed a similar performance with regard to heterotrophic kinetic and stoichiometric parameters and organic matter effluent concentrations. The influent was characterized by means of its COD fractions and the average removal percentages for COD concentrations were around 97% for both plants in spite of influent COD fluctuation, temperature variations and sludge retention time (SRT) evolution. Both SRT evolution and temperature affect the heterotrophic yield (YH) and the decay coefficient (bH) in the same range for both plants. YH values of over 0.8 mg COD/mg COD were obtained during the unsteady periods, while under steady state conditions these values fell to less than 0.4 mg COD/mg COD. bH by contrast reached values of less than 0.05 d−1.
Quality assessment of rooftop runoff and harvested rainwater from a building catchment
Authors: Lee J. Y. , Kim H. J. , Han M. Y.
Published: August 2011

A major obstacle to the promotion of rainwater harvesting is chemical and microbiological concerns. To determine its suitability as an alternative water resource, water quality parameters such as pH, turbidity and metal ion concentrations and counted total coliform, Escherichia coli and heterotrophic bacteria were measured. It was observed that the stored rainwater had a neutral average pH and that its turbidity depended on the duration and intensity of the rainfall event. Metal concentrations were within the permissible limits specified in the Korea drinking water standard. In addition, counts of coliform, E. coli and heterotrophic bacteria were higher in the first flush 5 min after the start of the rainfall event. Principal component analysis and correlation analysis through 40 events in 2009 showed that the quality of stored rainwater depends on the conditions of the catchment and storage tank and the antecedent dry period.
The Politics of Ambiguity in Asia's Sovereign Wealth Funds
Authors: Pekkanen Saadia M. , Tsai Kellee S.
Published: August 2011

Investments by Asian Sovereign Wealth Funds (SWFs) continue to be of concern in the global economy. A common perception is that they are managed by state-affiliated entities with geostrategic motives that could somehow prove detrimental to host countries. This paper demonstrates, however, that even the definition, let alone the establishment and investment targets, of Asian SWFs are embroiled in ambiguity within home country politics. Plainly put, ambiguity refers to the absence of clear-cut policy processes, means, and goals. How do we explain the ambiguity surrounding SWFs? Ambiguity is not a cover for deliberate, cohesive, and strategic actions, because SWFs are not under the control of any one set of actors. Rather, perhaps to the discomfort even of home country sovereigns, ambiguity is a messy domestic product of contending political forces that do not allow a marked trend toward any one single policy equilibrium on sovereign investments, whether domestic or foreign. While this reality is perhaps understandable in a democratic polity, it is also equally true of authoritarian ones in the region. In both types of cases, ambiguity is constructed inadvertently by the interactions of state, interstate, and intrastate actors, each with their own interests and expectations about the role of SWFs. Analyzing the cases in Singapore, China, Japan, and other Asian countries, from this unifying perspective suggests that while ambiguity may fuel external anxiety concerning home country intentions, it actually reflects far more domestically salient controversies about SWF activities than is typically appreciated.
Engineering the Incident Command and Multiagency Coordination Systems
Authors: Stambler Kimberly S , Barbera Joseph A
Published: August 2011

The initial development of the Incident Command System (ICS) and the Multiagency Coordination System (MACS) was conducted through an extensive research, development, and application program initiated by the United States Forest Service in the 1970s and 1980s. Known as FIRESCOPE (Firefighting Resources of California Organized for Potential Emergencies), the complex development process produced these two major management systems that are now central to the Department of Homeland Security’s National Incident Management System (NIMS). Despite the prominence of NIMS, very little information is widely available regarding the original research and development of ICS and MACS. This paper explores the extensive product development and consensus process used to create ICS and MACS, plus the implementation process that propagated these landmark systems that continue to expand in prominence for incident management and coordination during emergencies and disasters. Through analysis of historical documents and interviews with professionals centrally involved in FIRESCOPE, this paper chronicles the evolution of ICS and MACS, including a summary of their varied courses following the end of the original 10-year FIRESCOPE Program initiative.