bluuebunny/crossref_metadata_2025 huggingface dataset:
Review of Indications for Endotracheal Intubation in Burn Patients with Suspected Inhalational Injury
Authors: Concannon Elizabeth , Damkat Thomas Lindsay , Kerr Lachlan , Damkat Ivo , Reddi Benjamin , Greenwood John E. , Solanki Nicholas S. , Wagstaff Marcus J. D.
Published: March 2023

Inhalation injury is a major contributor to mortality following burn injury. Despite recognised clinical criteria to guide the intubation of burn patients, concerns remain regarding overutilisation of intubation. Complications can arise as a result of intubation, including ventilator-associated pneumonia (VAP). This study reviews the indications for intubation against the internationally accepted criteria (American Burns Association (ABA) and Denver criteria) for burn patients treated at the Royal Adelaide Hospital (RAH) burns unit between 2017 and 2020. Burn patients who were intubated on arrival to the RAH or in a pre-hospital setting were identified using the BRANZ database. Indications for intubation were compared to the ABA and Denver criteria. A total of 61 patients were identified with a mean total body surface area of 17.8%. A total of 95% of patients met one of the ABA and Denver criteria. The most common ABA and Denver criteria for intubation was deep facial burns or singed facial hair, respectively. Most adult patients with burns admitted to the RAH are intubated per published criteria. Early nasoendoscopy/bronchoscopy may be useful in determining patients who can be safely extubated within 48 h.
Inspection Digital Literacy for School Improvement
Authors: Martínez-Serrano María del Carmen , Romero-García Manuel Angel , García-Martínez Inmaculada , Gavín-Chocano Óscar
Published: March 2023

Educational inspection, as an essential part of the current educational environment, supports its mission through more pragmatic and comprehensive processes, techniques, and models, which guarantee the right of students to quality education. The aim of the present study was to determine the causal effect of gender and age on the dimensions of the instrument in the inspector population. Specifically, 118 male and female inspectors from the Educational Inspection Service of Andalusia (Spain) participated, with an average age of 47.56 years (±5.70). In terms of gender, 30 were women (25.40%) and 88 were men (74.60%). An instrument was developed specifically for this study with the purpose of assessing the participants’ opinions of the extent to which their work contributes to educational improvement. The results evidenced the relationship between the dimensions of the instrument: attention to members of the educational community (AMEC), supervision of guidance and tutorial action (SGTA), attention and inclusion of diversity (AID), and technological resources (TR) (p < 0.01). Similarly, the multigroup model obtained good structural validity (χ2 = 68.180; RMSEA = 0.078; GFI = 0.923; CFI = 0.959; IFI = 0.967). In terms of gender, no significant differences were obtained, although the results were moderately superior among males compared to females. In relation to age, younger inspectors had better TR results, and older inspectors had better AMEC and SGTA results. The conclusions strengthen the importance of the Education Inspection Service in educational establishments, highlighting the need to supervise the processes of attention and inclusion for diversity. A great deal of resistance was observed, especially as there is a lack of training in information and communication technology (ICT).
Investigation on High-Viscosity Chemical Waste Liquid Atomizer Based on VOF-DPM
Authors: Ou Haoyu , Su Lei , Shi Yang , Ruan Shijie
Published: March 2023

The viscosity of adiponitrile waste liquid is as high as 1000 cp. It is challenging to spray and atomize the waste liquid normally. Based on the coaxial three-channel pneumatic atomizer, a two-stage supersonic steam atomizer is proposed in this paper, and the atomization process is simulated by Fluent software. Compared with the traditional atomization simulation method, the Volume-of-Fluid to Discrete-Phase-Model (VOF-DPM) bi-directional coupling model and Adaptive Mesh Refinement (AMR) technology can save mesh and improve the computational efficiency. The atomization processes of primary breakup and secondary breakup are entirely captured and analyzed. The results show that the Sauter Mean Diameter (SMD) is about 116–180 μm, the SMD decreases with the increase of steam inlet absolute pressure, and the atomization quality can meet the combustion requirements. This study can be used for the performance optimization of the high-viscosity liquid atomizers in the chemical and aerospace industry and shorten the time engineers spend in the simulation calculation to verify the rationality of the structure.
Synaptosomes: A Functional Tool for Studying Neuroinflammation
Authors: Trebesova Hanna , Grilli Massimo
Published: March 2023

Synaptosomes are subcellular components isolated from nerve terminations that can be prepared by homogenizing brain tissue in isotonic sucrose solution followed by appropriate centrifugation. Their preparation technique has a long history since synaptosomes were first isolated from nerve endings and described by Gray and Whittaker in 1962. The preparation of synaptosomes produces presynaptic boutons alone or in combination with fragments of postsynaptic membranes. Interestingly, synaptosomes contain organelles and vesicles that express native channels, receptors, and transporters. At 37 °C, these isolated nerve endings are metabolically active and synthesize and release neurotransmitters. They are actively used to investigate neurotransmission, its actors, and the mechanisms of neurotransmitter release. To date, many functional and non-functional applications of synaptosomes have been documented. Due to their versatility, synaptosomes have been actively used to study neuroinflammatory processes.
Inferences about Fishing Impacts on the Reproductive Pattern of Torroto Grunt—Genyatremus luteus (BLOCH, 1790)—From the Amazon Continental Shelf
Authors: Fernandes Suelly , Pinheiro Larissa , Bentes Bianca
Published: March 2023

Genyatremus luteus’ reproductive dynamics were investigated by examining the gonadal first maturation length (L50) and reproductive period (gonadosomatic index, GSI, and relative condition factor, Kr) in relation to the environmental factor rainfall. Sampling was conducted monthly from April 2016 to April 2017, in five areas (A1 to A5) using gill nets and fixed traps. The sex ratio distribution of the 331 specimens captured (174 females, 140 males, and 17 with undetermined sex) did not differ between months. Females were larger (19.28 cm vs. 16.63 cm to males) and heavier (147.21 g vs. 92.14 g to males) and, for both sexes, the means of Kr and GSI indicated the probable spawning period of the species that occurred from June to September, after a decrease in the precipitation of the region. The value of L50 was estimated as 15.13 cm for females, 14.29 cm for males, and 14.78 cm for both sexes. In the rainy season, the behavior of the species is associated with breeding in certain areas but in the dry season, it is related to the fattening and refuge of juveniles and adults. This research shows the first insight for fishing management about a species that requires different ecosystems to complete their life cycle.
Socio-Ecological Overview of the Greater Amberjack Fishery in the Balearic Islands
Authors: Pereira Inês R. , Valls Maria , Aoufi Sofya , Bienentreu Oona C. , Huang Yansong , Cabanellas-Reboredo Miguel
Published: March 2023

This study provides the first socio-ecological overview of the Seriola dumerili fishery occurring in the Balearic Islands. This pelagic top-predator is among the five most valued fish resources of the Balearic community. Despite its ecological importance and potential vulnerability to aggregation fishing, few studies address the ecology of this large Carangidae species. Shining a light on its ecology is vital to ensure adequate species conservation and the sustainable and effective management of the fishery. Historical catches from 1950–1999, alongside detailed landing data for the last 21 years, were analysed to identify potential patterns in ecological and socio-economic factors. Significant inter-annual variability among the years was found in historical catches of greater amberjack, while catches and mean prices of the different size categories revealed significant results between seasons and months, respectively. Additionally, the purse seine fleet accounted for the highest percentage of S. dumerili catches. CPUE did not appear to change greatly between months and years after the annual 8-month fishing ban imposed in 2011 and therefore a re-evaluation of the closure was intended. Overall, this study suggests seasonality influences the S. dumerili fishery in the Balearic Islands, within which ecological influences show a higher regulating power than socio-economic factors.
Hidden Compositional Heterogeneity of Fish Chromosomes in the Era of Polished Genome Assemblies
Authors: Vohnoutová Marta , Žifčáková Lucia , Symonová Radka
Published: March 2023

Fish chromosomes are considered homogeneous in their AT/GC nucleotide composition, and banding patterns enabling identification of homologs are largely missing. While cytogenomic approaches try to compensate for this issue by virtual karyotyping, they rely on the quality of genome assemblies available. Recently, soft-masked genome assemblies combining costly and arduous long- and short-read sequencing and new generation assemblers became available for two teleost fish species, climbing perch (Anabas testudineus) and channel bull blenny (Cottoperca gobio). Soft-masking turns repetitive sequences in a genome assembly into lower case letters, leaving unique sequences in upper case. This enables investigators to assess the proportion of guanine and cytosine nucleotides (GC%) of transposable elements as an indicator of AT/GC homogenisation in fish. We have developed a new version of our Python tool Evan, which utilises chromosome-level genome assemblies and combines the profiles of GC% and the proportion of repeats (rep%) along chromosomes. Our profiles of both of those fishes showed clear and abrupt but small-scale fluctuations in GC% along otherwise compositionally homogenised sequences. Our study also highlights the key role of the sliding window size in determining the resolution of GC% profiling. While the quality of the genome assemblies appeared to be sufficient for GC%/rep% profiling, more effective repeat masking is necessary to better distinguish to what extent repeats compositionally homogenize fish genomes.
Fekete–Szegö Problem and Second Hankel Determinant for a Class of Bi-Univalent Functions Involving Euler Polynomials
Authors: Riaz Sadia , Shaba Timilehin Gideon , Xin Qin , Tchier Fairouz , Khan Bilal , Malik Sarfraz Nawaz
Published: March 2023

Some well-known authors have extensively used orthogonal polynomials in the framework of geometric function theory. We are motivated by the previous research that has been conducted and, in this study, we solve the Fekete–Szegö problem as well as give bound estimates for the coefficients and an upper bound estimate for the second Hankel determinant for functions in the class GΣ(v,σ) of analytical and bi-univalent functions, implicating the Euler polynomials.
Investigation of Fractional Order Dynamics of Tuberculosis under Caputo Operator
Authors: Ullah Ihsan , Ahmad Saeed , Arfan Muhammad , De la Sen Manuel
Published: March 2023

In this article, a new deterministic disease system is constructed to study the influence of treatment adherence as well as awareness on the spread of tuberculosis (TB). The suggested model is composed of six various classes, whose dynamics are discussed in the sense of the Caputo fractional operator. Firstly the model existence of a solution along with a unique solution is checked to determine whether the system has a solution or not. The stability of a solution is also important, so we use the Ulam–Hyers concept of stability. The approximate solution analysis is checked by the technique of Laplace transformation using the Adomian decomposition concept. Such a solution is in series form which is decomposed into smaller terms and the next term is obtained from the previous one. The numerical simulation is established for the obtained schemes using different fractional orders along with a comparison of classical derivatives. Such an analysis will be helpful for testing more dynamics instead of only one type of integer order discussion.
Risk Perception, Perceived Government Coping Validity, and Individual Sleep Problems in the Early Stage of the COVID-19 Pandemic in China: Mediation Analysis Based on Negative Emotions
Authors: Xu Tao , Zheng Kai , Wu Xiaoqin
Published: March 2023

This study aimed to investigate the relationship among risk perception, negative emotions, perceived government coping validity, and the sleep problem of the public, through regression analysis and mediation analysis of data from the early stages of the COVID-19 outbreak in China (three months after the outbreak). It found that people’s perception of the risk of the pandemic, negative emotions, and perceived government coping validity significantly affected people’s sleep quality and nightmares. Further analysis found that individuals’ perception of risk not only affected their sleep but also intensified their negative emotions, ultimately impairing the quality of their sleep and leading to nightmares. However, having a high level of coping validity can mitigate negative emotions and consequently decrease the occurrence of nightmares, thereby enhancing the quality of sleep. Specifically, perceived government coping validity could not only directly reduce nightmares, but also indirectly reduce nightmares by lowering negative emotions. However, it could only indirectly improve sleep by reducing negative emotions. It implicated that improving and resolving sleep problems required not only medical intervention but also psychological intervention. Simultaneously, improving the government’s response effectiveness could strengthen people’s trust in the government, stabilize their mental states, and significantly improve their quality of life by reducing negative emotions and improving sleep.
Biological, Psychiatric, Psychosocial, and Cognitive Factors of Poststroke Depression
Authors: Dulay Mario F. , Criswell Amber , Hodics Timea M.
Published: March 2023

Background: Depression is the most common psychiatric condition that occurs after cerebrovascular accident, especially within the first year after stroke. Poststroke depression (PSD) may occur due to environmental factors such as functional limitations in daily activities, lower quality of life, or biological factors such as damage to areas in the brain involved in emotion regulation. Although many factors are hypothesized to increase the risk of PSD, the relative contribution of these factors is not well understood. Purpose: We evaluated which cross-sectional variables were associated with increased odds of PSD in our adult outpatient stroke neuropsychology clinic population. Methods: The sample included 325 patients (49.2% female; mean age of 59-years old) evaluated at an average of 8.1 months after an ischemic or hemorrhagic stroke. Variables included in logistic regression were stroke characteristics, demographics, psychosocial factors, comorbid medical problems, comorbid psychiatric conditions, and cognitive status. The Mini International Neuropsychiatric Inventory was used to determine DSM-defined PSD and anxiety disorders. A standard neuropsychological test battery was administered. Results: PSD occurred in 30.8% of the sample. Logistic regression indicated that increased odds of PSD were associated with a comorbid anxiety disorder (5.9 times more likely to suffer from PSD, p < 0.001). Further, increased odds of PSD were associated with a history of depression treatment before stroke (3.0 times more likely to suffer from PSD), fatigue (2.8 times more likely), memory impairment (2.4 times more likely), and younger age at stroke (all p values < 0.006). Discussion: Results suggest that PSD is likely multifactorial and extends the literature by demonstrating that a comorbid anxiety disorder correlated strongest with PSD. Poststroke screening and treatment plans should address not only depression but comorbid anxiety.
The Response of Triple-Negative Breast Cancer to Neoadjuvant Chemotherapy and the Epithelial–Mesenchymal Transition
Authors: Zapperi Stefano , La Porta Caterina A. M.
Published: March 2023

It would be highly desirable to find prognostic and predictive markers for triple-negative breast cancer (TNBC), a strongly heterogeneous and invasive breast cancer subtype often characterized by a high recurrence rate and a poor outcome. Here, we investigated the prognostic and predictive capabilities of ARIADNE, a recently developed transcriptomic test focusing on the epithelial–mesenchymal transition. We first compared the stratification of TNBC patients obtained by ARIADNE with that based on other common pathological indicators, such as grade, stage and nodal status, and found that ARIADNE was more effective than the other methods in dividing patients into groups with different disease-free survival statistics. Next, we considered the response to neoadjuvant chemotherapy and found that the classification provided by ARIADNE led to statistically significant differences in the rates of pathological complete response within the groups.
Osteogenic Potential of Autologous Dentin Graft Compared with Bovine Xenograft Mixed with Autologous Bone in the Esthetic Zone: Radiographic, Histologic and Immunohistochemical Evaluation
Authors: Oguić Matko , Čandrlić Marija , Tomas Matej , Vidaković Bruno , Blašković Marko , Jerbić Radetić Ana Terezija , Zoričić Cvek Sanja , Kuiš Davor , Cvijanović Peloza Olga
Published: March 2023

This prospective, randomized, controlled clinical trial reports clinical, radiographic, histologic and immunohistochemical results of autologous dentin graft (ADG) and its comparison with a mixture of bovine xenograft with autologous bone (BX+AB). After tooth extraction in the esthetic zone of maxilla, the alveolar ridge of 20 patients in the test group was augmented with ADG, while 17 patients in the control group received the combination of BX+AB. Cone beam computed tomography (CBCT) was performed before tooth extraction and after 4 months when a total of 22 bone biopsies were harvested and subjected to histological and immunohistochemical analysis. Radiological analysis showed comparable results of bone dimension loss in both groups. Quantitative histologic analysis showed comparable results with no statistically significant differences between the groups. Immunohistochemical staining with TNF-α and BMP-4 antibodies revealed immunopositivity in both groups. A statistically significant difference between the groups was found in the intensity of TNF-α in the area of newly formed bone (p = 0.0003) and around remaining biomaterial particles (p = 0.0027), and in the intensity of BMP-4 in the area around biomaterial particles (p = 0.0001). Overall, ADG showed biocompatibility and achieved successful bone regeneration in the esthetic zone of the maxilla similar to BX+AB.
Heteroligand Iron(V) Complexes Containing Porphyrazine, trans-Di[benzo]porphyrazine or Tetra[benzo]porphyrazine, Oxo and Fluoro Ligands: DFT Quantum-Chemical Study
Authors: Chachkov Denis V. , Mikhailov Oleg V.
Published: March 2023

By using quantum chemical calculation data obtained by the DFT method with the B3PW91/TZVP and OPBE/TZVP levels, the possibility of the existence of three Fe(V) complexes, each of which contains in the inner coordination sphere porphyrazine/trans-di[benzo]porphyrazine/tetra[benzo]porphyrazine (phthalocyanine), oxygen (O2−) and fluorine (F−) ions, was shown. Key geometric parameters of the molecular structure of these heteroligand complexes are given; it is noted that FeN4 chelate nodes, and all metal-chelate and non-chelate cycles in each of these compounds, are practically planar with the deviation from coplanarity, as a rule, by no more than 0.5°. Furthermore, the bond angles between two nitrogen atoms and an Fe atom are equal to 90°, or less than this by no more than 0.1°, while the bond angles between donor atoms N, Fe, and O or F, in most cases, albeit insignificantly, differ from this value. Nevertheless, the bond angles formed by Fe, O and F atoms are exactly 180°. It is shown that good agreement occurs between the structural data obtained using the above two versions of the DFT method. NBO analysis data for these complexes are presented; it is noted that, according to both DFT methods used, the ground state of the each of three complexes under consideration may be a spin quartet or spin doublet. Additionally, standard thermodynamic parameters of formation (standard enthalpy ∆fH0, entropy S0 and Gibbs’s energy ∆fG0) for the macrocyclic compounds under consideration are calculated.
Age- and Sex-Specific Association between Lipoprotein-Related Phospholipase A2 and Cardiometabolic Risk Factors
Authors: Ke Pin-Hsuan , Chen Jau-Yuan , Chen Yi-Hsuan , Yeh Wei-Chung , Li Wen-Cheng
Published: March 2023

(1) Lipoprotein-associated phospholipase A2 (Lp-PLA2) is a risk factor for predicting cardiovascular diseases. Metabolic syndrome is characterized by a state of chronic inflammation that is related to an increased risk of cardiovascular events and death. In the present study, we aimed to analyze the correlation between cardiometabolic risk factors and Lp-PLA2 levels. (2) We collected the related retrospective medical data of Chinese adults, of which 3983 were men and 2836 were women (aged ≥ 18 years), who underwent health check-ups, and discussed the sex and age-related differences. (3) Data analysis showed that Lp-PLA2 was significantly related to lipoproteins and glutamic pyruvic transaminase (GPT), and that a linear trend was observed with increasing Lp-PLA2 levels for all ages and sexes. However, fasting glucose was significantly related to Lp-PLA2 only in the younger population. The two obesity-related parameters (waist-to-height ratio and waist circumference) also had a greater correlation with Lp-PLA2 levels in the younger groups; however, the correlation weakened in the elderly population. Meanwhile, the correlation between mean arterial pressure and creatinine level and Lp-PLA2 was significant only in younger men. (4) The results show that the expression patterns of Lp-PLA2 differ between sexes and across age groups.
Effects of Human LAV-BPIFB4 Gene Therapy on the Epigenetic Clock and Health of Aged Mice
Authors: Giuliani Maria Elisa , Barbi Veronica , Bigossi Giorgia , Marcozzi Serena , Giacconi Robertina , Cardelli Maurizio , Piacenza Francesco , Orlando Fiorenza , Ciaglia Elena , Cattaneo Monica , Mongelli Alessia , Gaetano Carlo , Provinciali Mauro , Puca Annibale Alessandro , Malavolta Marco
Published: March 2023

The homozygous genotype of the Longevity-Associated Variant (LAV) in Bactericidal/Permeability-Increasing Fold-Containing Family B member 4 (BPIFB4) is enriched in long-living individuals of three independent populations and its genetic transfer in C57BL/6J mice showed a delay in frailty progression and improvement of several biomarkers of aging and multiple aspects of health. The C57BL/6J strain is a suitable model for studying therapies aimed at extending healthy aging and longevity due to its relatively short lifespan and the availability of aging biomarkers. Epigenetic clocks based on DNA methylation profiles are reliable molecular biomarkers of aging, while frailty measurement tools are used to evaluate overall health during aging. In this study, we show that the systemic gene transfer of LAV-BPIFB4 in aged C57BL/6J mice was associated with a significant reduction in the epigenetic clock-based biological age, as measured by a three CpG clock method. Furthermore, LAV-BPIFB4 gene transfer resulted in an improvement of the Vitality Score with a reduction in the Frailty Index. These findings further support the use of LAV-BPIFB4 gene therapy to induce beneficial effects on epigenetic mechanisms associated with aging and frailty in aged mice, with potential implications for future therapies to prevent frailty in humans.
Kappa-Carrageenan/Chitosan/Gelatin Scaffolds Provide a Biomimetic Microenvironment for Dentin-Pulp Regeneration
Authors: Loukelis Konstantinos , Machla Foteini , Bakopoulou Athina , Chatzinikolaidou Maria
Published: March 2023

This study aims to investigate the impact of kappa-carrageenan on dental pulp stem cells (DPSCs) behavior in terms of biocompatibility and odontogenic differentiation potential when it is utilized as a component for the production of 3D sponge-like scaffolds. For this purpose, we prepared three types of scaffolds by freeze-drying (i) kappa-carrageenan/chitosan/gelatin enriched with KCl (KCG-KCl) as a physical crosslinker for the sulfate groups of kappa-carrageenan, (ii) kappa-carrageenan/chitosan/gelatin (KCG) and (iii) chitosan/gelatin (CG) scaffolds as a control. The mechanical analysis illustrated a significantly higher elastic modulus of the cell-laden scaffolds compared to the cell-free ones after 14 and 28 days with values ranging from 25 to 40 kPa, showing an increase of 27–36%, with the KCG-KCl scaffolds indicating the highest and CG the lowest values. Cell viability data showed a significant increase from days 3 to 7 and up to day 14 for all scaffold compositions. Significantly increasing alkaline phosphatase (ALP) activity has been observed over time in all three scaffold compositions, while the KCG-KCl scaffolds indicated significantly higher calcium production after 21 and 28 days compared to the CG control. The gene expression analysis of the odontogenic markers DSPP, ALP and RunX2 revealed a two-fold higher upregulation of DSPP in KCG-KCl scaffolds at day 14 compared to the other two compositions. A significant increase of the RunX2 expression between days 7 and 14 was observed for all scaffolds, with a significantly higher increase of at least twelve-fold for the kappa-carrageenan containing scaffolds, which exhibited an earlier ALP gene expression compared to the CG. Our results demonstrate that the integration of kappa-carrageenan in scaffolds significantly enhanced the odontogenic potential of DPSCs and supports dentin-pulp regeneration.
Role of Inflammatory Biomarkers (NLR, LMR, PLR) in the Prognostication of Malignancy in Indeterminate Thyroid Nodules
Authors: Gambardella Claudio , Mongardini Federico Maria , Paolicelli Maddalena , Bentivoglio Davide , Cozzolino Giovanni , Ruggiero Roberto , Pizza Alessandra , Tolone Salvatore , del Genio Gianmattia , Parisi Simona , Brusciano Luigi , Cerbara Loredana , Docimo Ludovico , Lucido Francesco Saverio
Published: March 2023

Indeterminate follicular thyroid lesions (Thyr 3A and 3B) account for 10% to 30% of all cytopathologic diagnoses, and their unpredictable behavior represents a hard clinical challenge. The possibility to preoperatively predict malignancy is largely advocated to establish a tailored surgery, preventing diagnostic thyroidectomy. We analyzed the role of the neutrophil-to-lymphocyte ratio (NLR), the platelet-to-lymphocyte ratio (PLR) and the lymphocyte-to-monocyte ratio (LMR) as prognostic factors of malignancy for indeterminate thyroid nodules. In patients affected by cytological Thyr 3A/3B nodules, NLR, PLR and LMR were retrospectively compared and correlated with definitive pathology malignancy, utilizing student’s t-test, ROC analysis and logistic regression. One-hundred and thirty-eight patients presented a Thyr 3A and 215 patients presented a Thyr 3B. After the logistic regression, in Thyr 3A, none of the variables were able to predict malignancy. In Thyr 3B, NLR prognosticated thyroid cancer with an AUC value of 0.685 (p < 0.0001) and a cut-off of 2.202. The NLR results were also similar when considering the overall cohort. The use of cytological risk stratification in addressing the management of indeterminate thyroid nodules in patients is not always reliable. NLR is an easy and reproducible inflammatory biomarker capable of improving the accuracy of preoperative prognostication of malignancy.
Zebrafish: A Relevant Genetic Model for Human Primary Immunodeficiency (PID) Disorders?
Authors: Basheer Faiza , Sertori Robert , Liongue Clifford , Ward Alister C.
Published: March 2023

Primary immunodeficiency (PID) disorders, also commonly referred to as inborn errors of immunity, are a heterogenous group of human genetic diseases characterized by defects in immune cell development and/or function. Since these disorders are generally uncommon and occur on a variable background profile of potential genetic and environmental modifiers, animal models are critical to provide mechanistic insights as well as to create platforms to underpin therapeutic development. This review aims to review the relevance of zebrafish as an alternative genetic model for PIDs. It provides an overview of the conservation of the zebrafish immune system and details specific examples of zebrafish models for a multitude of specific human PIDs across a range of distinct categories, including severe combined immunodeficiency (SCID), combined immunodeficiency (CID), multi-system immunodeficiency, autoinflammatory disorders, neutropenia and defects in leucocyte mobility and respiratory burst. It also describes some of the diverse applications of these models, particularly in the fields of microbiology, immunology, regenerative biology and oncology.
Molecular Mechanisms, Diagnosis and Treatments in Digestive Malignancy
Authors: Kanda Tatsuo , Masuzaki Ryota , Sasaki-Tanaka Reina , Kogure Hirofumi , Moriyama Mitsuhiko
Published: March 2023

In this Special Issue, “Molecular Mechanisms, Diagnosis and Treatments in Digestive Malignancy”, of the International Journal of Molecular Sciences, a total of 10 impactful articles have been published [...]
Flavonoid Synthesis-Related Genes Determine the Color of Flower Petals in Brassica napus L.
Authors: Li Shijun , Li Xi , Wang Xiaodan , Chang Tao , Peng Zechuan , Guan Chunyun , Guan Mei
Published: March 2023

The color of rapeseed (Brassica napus L.) petal is usually yellow but can be milky-white to orange or pink. Thus, the petal color is a popular target in rapeseed breeding programs. In his study, metabolites and RNA were extracted from the yellow (Y), yellow/purple (YP), light purple (LP), and purple (P) rapeseed petals. Ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), RNA-Seq, and quantitative real-time (qRT-PCR) analyses were performed to analyze the expression correlation of differential metabolites and differential genes. A total of 223 metabolites were identified in the petals of the three purple and yellow rapeseed varieties by UPLC-MS/MS. A total of 20511 differentially expressed genes (DEGs) between P, LP, YP, versus Y plant petals were detected. This study focused on the co-regulation of 4898 differential genes in the three comparison groups. Kyoto Encyclopedia of Genes and Genomes (KEGG) functional annotation and quantitative RT-PCR analysis showed that the expression of BnaA10g23330D (BnF3'H) affects the synthesis of downstream peonidin and delphinidin and is a key gene regulating the purple color of petals in B. napus. L. The gene may play a key role in regulating rapeseed flower color; however, further studies are needed to verify this. These results deepen our understanding of the molecular mechanisms underlying petal color and provide the theoretical and practical basis for flower breeding targeting petal color.
Molecular and Functional Characteristics of Airway Epithelium under Chronic Hypoxia
Authors: Wong Sharon L. , Kardia Egi , Vijayan Abhishek , Umashankar Bala , Pandzic Elvis , Zhong Ling , Jaffe Adam , Waters Shafagh A.
Published: March 2023

Localized and chronic hypoxia of airway mucosa is a common feature of progressive respiratory diseases, including cystic fibrosis (CF). However, the impact of prolonged hypoxia on airway stem cell function and differentiated epithelium is not well elucidated. Acute hypoxia alters the transcription and translation of many genes, including the CF transmembrane conductance regulator (CFTR). CFTR-targeted therapies (modulators) have not been investigated in vitro under chronic hypoxic conditions found in CF airways in vivo. Nasal epithelial cells (hNECs) derived from eight CF and three non-CF participants were expanded and differentiated at the air–liquid interface (26–30 days) at ambient and 2% oxygen tension (hypoxia). Morphology, global proteomics (LC-MS/MS) and function (barrier integrity, cilia motility and ion transport) of basal stem cells and differentiated cultures were assessed. hNECs expanded at chronic hypoxia, demonstrating epithelial cobblestone morphology and a similar proliferation rate to hNECs expanded at normoxia. Hypoxia-inducible proteins and pathways in stem cells and differentiated cultures were identified. Despite the stem cells’ plasticity and adaptation to chronic hypoxia, the differentiated epithelium was significantly thinner with reduced barrier integrity. Stem cell lineage commitment shifted to a more secretory epithelial phenotype. Motile cilia abundance, length, beat frequency and coordination were significantly negatively modulated. Chronic hypoxia reduces the activity of epithelial sodium and CFTR ion channels. CFTR modulator drug response was diminished. Our findings shed light on the molecular pathophysiology of hypoxia and its implications in CF. Targeting hypoxia can be a strategy to augment mucosal function and may provide a means to enhance the efficacy of CFTR modulators.
Reactive Oxygen Species in the Aorta and Perivascular Adipose Tissue Precedes Endothelial Dysfunction in the Aorta of Mice with a High-Fat High-Sucrose Diet and Additional Factors
Authors: Osaki Ayumu , Kagami Kazuki , Ishinoda Yuki , Sato Atsushi , Kimura Toyokazu , Horii Shunpei , Ito Kei , Toya Takumi , Ido Yasuo , Namba Takayuki , Masaki Nobuyuki , Nagatomo Yuji , Adachi Takeshi
Published: March 2023

Metabolic syndrome (Mets) is the major contributor to the onset of metabolic complications, such as hypertension, type 2 diabetes mellitus (DM), dyslipidemia, and non-alcoholic fatty liver disease, resulting in cardiovascular diseases. C57BL/6 mice on a high-fat and high-sucrose diet (HFHSD) are a well-established model of Mets but have minor endothelial dysfunction in isolated aortas without perivascular adipose tissue (PVAT). The purpose of this study was to evaluate the effects of additional factors such as DM, dyslipidemia, and steatohepatitis on endothelial dysfunction in aortas without PVAT. Here, we employed eight-week-old male C57BL/6 mice fed with a normal diet (ND), HFHSD, steatohepatitis choline-deficient HFHSD (HFHSD-SH), and HFHSD containing 1% cholesterol and 0.1% deoxycholic acid (HFHSD-Chol) for 16 weeks. At week 20, some HFHSD-fed mice were treated with streptozocin to develop diabetes (HFHSD-DM). In PVAT-free aortas, the endothelial-dependent relaxation (EDR) did not differ between ND and HFHSD (p = 0.25), but in aortas with PVAT, the EDR of HFHSD-fed mice was impaired compared with ND-fed mice (p = 0.005). HFHSD-DM, HFHSD-SH, and HFHSD-Chol impaired the EDR in aortas without PVAT (p < 0.001, p = 0.019, and p = 0.009 vs. ND, respectively). Furthermore, tempol rescued the EDR in those models. In the Mets model, the EDR is compromised by PVAT, but with the addition of DM, dyslipidemia, and SH, the vessels themselves may result in impaired EDR.
Oral Cladribine Impairs Intermediate, but Not Conventional, Monocyte Transmigration in Multiple Sclerosis Patients across a Model Blood-Brain Barrier
Authors: Lin Linda Y. , Juillard Pierre , Hawke Simon , Marsh-Wakefield Felix , Grau Georges E.
Published: March 2023

Multiple sclerosis (MS) is a disease in which the immune system damages components of the central nervous system (CNS), leading to the destruction of myelin and the formation of demyelinating plaques. This often occurs in episodic “attacks” precipitated by the transmigration of leukocytes across the blood-brain barrier (BBB), and repeated episodes of demyelination lead to substantial losses of axons within and removed from plaques, ultimately leading to progressive neurological dysfunction. Within leukocyte populations, macrophages and T and B lymphocytes are the predominant effectors. Among current immunotherapies, oral cladribine’s impact on lymphocytes is well characterised, but little is known about its impact on other leukocytes such as monocytes and dendritic cells (DCs). The aim of this study was to determine the transmigratory ability of monocyte and DC subsets in healthy subjects and untreated and cladribine-treated relapse-remitting MS (RRMS) patients using a well-characterised model of the BBB. Peripheral blood mononuclear cells from subjects were added to an in vitro transmigration assay to assess cell migration. Our findings show that while prior treatment with oral cladribine inhibits the migration of intermediate monocytes, it has no impact on the transmigration of DC subsets. Overall, our data indicate a previously unrecognised role of cladribine on intermediate monocytes, known to accumulate in the brain active MS lesions.
Roles of TGF-β1 in Viral Infection during Pregnancy: Research Update and Perspectives
Authors: Trinh Quang Duy , Pham Ngan Thi Kim , Takada Kazuhide , Ushijima Hiroshi , Komine-Aizawa Shihoko , Hayakawa Satoshi
Published: March 2023

Transforming growth factor-beta 1 (TGF-β1) is a pleiotropic growth factor playing various roles in the human body including cell growth and development. More functions of TGF-β1 have been discovered, especially its roles in viral infection. TGF-β1 is abundant at the maternal–fetal interface during pregnancy and plays an important function in immune tolerance, an essential key factor for pregnancy success. It plays some critical roles in viral infection in pregnancy, such as its effects on the infection and replication of human cytomegalovirus in syncytiotrophoblasts. Interestingly, its role in the enhancement of Zika virus (ZIKV) infection and replication in first-trimester trophoblasts has recently been reported. The above up-to-date findings have opened one of the promising approaches to studying the mechanisms of viral infection during pregnancy with links to corresponding congenital syndromes. In this article, we review our current and recent advances in understanding the roles of TGF-β1 in viral infection. Our discussion focuses on viral infection during pregnancy, especially in the first trimester. We highlight the mutual roles of viral infection and TGF-β1 in specific contexts and possible functions of the Smad pathway in viral infection, with a special note on ZIKV infection. In addition, we discuss promising approaches to performing further studies on this topic.
Nutrition Genomics
Authors: Osada Jesús
Published: March 2023

This Special Issue is devoted to nutrition genomics, which is the characterization of the whole genome response to nutrients, in an effort to gather all the available pertinent information and to establish the foundation for a future encyclopedia of genomic responses driven by diets or nutrients [...]
Effects of Mineralocorticoid Receptor Blockade and Statins on Kidney Injury Marker 1 (KIM-1) in Female Rats Receiving L-NAME and Angiotensin II
Authors: Huang Jiayan , Caliskan Guzelce Ezgi , Gholami Shadi K. , Gawelek Kara L. , Mitchell Richard N. , Pojoga Luminita H. , Romero Jose R. , Williams Gordon H. , Adler Gail K.
Published: March 2023

Kidney injury molecule-1 (KIM-1) is a biomarker of renal injury and a predictor of cardiovascular disease. Aldosterone, via activation of the mineralocorticoid receptor, is linked to cardiac and renal injury. However, the impact of mineralocorticoid receptor activation and blockade on KIM-1 is uncertain. We investigated whether renal KIM-1 is increased in a cardiorenal injury model induced by L-NAME/ANG II, and whether mineralocorticoid receptor blockade prevents the increase in KIM-1. Since statin use is associated with lower aldosterone, we also investigated whether administering eiSther a lipophilic statin (simvastatin) or a hydrophilic statin (pravastatin) prevents the increase in renal KIM-1. Female Wistar rats (8–10 week old), consuming a high salt diet (1.6% Na+), were randomized to the following conditions for 14 days: control; L-NAME (0.2 mg/mL in drinking water)/ANG II (225 ug/kg/day on days 12–14); L-NAME/ANG II + eplerenone (100 mg/kg/day p.o.); L-NAME/ANG II + pravastatin (20 mg/kg/day p.o.); L-NAME/ANG II + simvastatin (20 mg/kg/day p.o.). Groups treated with L-NAME/ANG II had significantly higher blood pressure, plasma and urine aldosterone, cardiac injury/stroke composite score, and renal KIM-1 than the control group. Both eplerenone and simvastatin reduced 24-h urinary KIM-1 (p = 0.0046, p = 0.031, respectively) and renal KIM-1 immunostaining (p = 0.004, p = 0.037, respectively). Eplerenone also reduced renal KIM-1 mRNA expression (p = 0.012) and cardiac injury/stroke composite score (p = 0.04). Pravastatin did not affect these damage markers. The 24-h urinary KIM-1, renal KIM-1 immunostaining, and renal KIM-1 mRNA expression correlated with cardiac injury/stroke composite score (p < 0.0001, Spearman ranked correlation = 0.69, 0.66, 0.59, respectively). In conclusion, L-NAME/ANG II increases renal KIM-1 and both eplerenone and simvastatin blunt this increase in renal KIM-1.
Environmental Impact Analysis of Alkali-Activated Concrete with Fiber Reinforcement
Authors: Chottemada Pujitha Ganapathi , Kar Arkamitra , Kara De Maeijer Patricia
Published: March 2023

The scientific community is shifting its focus towards construction materials with a low carbon footprint, such as alkali-activated concrete (AAC). The present study conducts an environmental impact assessment using the cradle-to-grave approach to examine the environmental implications of three different types of ambient-cured AACs with varied combinations of precursors. The 28-day compressive strength values of the concrete mixes used for comparing environmental impacts vary from 35 to 55 MPa. Among these mixtures, the one with the least environmental impact is chosen for further impact assessment with the inclusion of fibers. Three different fiber reinforced AAC mixes containing 0.3% steel, glass, and polypropylene fibers, respectively by volume of AAC, are used in this study. The findings show that Portland Cement concrete has 86% and 34% higher impacts than AAC on the ecosystem and human health, respectively. In the production of AAC, sodium silicate is found to have the highest impact on the environment, in the range of 30–50% of the total impact. Among the various fibers used, glass fibers have the highest impact, which is 12% and 13% higher than that of the plain AAC mix, on the ecosystem and human health, respectively.
Effect of Ru on Deformation Mechanism and Microstructure Evolution of Single-Crystal Superalloys under Medium-Temperature and High-Stress Creep
Authors: Emokpaire Stephen Okhiai , Wang Nan , Liu Jide , Zhu Chongwei , Wang Xinguang , Li Jinguo , Zhou Yizhou
Published: March 2023

In this work, the effect of the Ru element on the γ′-phase evolution and deformation mechanism in the fourth-generation Ni-based single-crystal superalloy was investigated. Results show that the Ru element alters the distribution coefficient of other elements in the alloy to produce reverse partitioning behavior, which leads to a difference in microstructure between 0Ru and 3Ru. The addition of Ru triggered the incubation period before the beginning of the primary creep stage, which depends on the creep temperature and stress during creep deformation. TEM results revealed that Ru addition inhibits the slip system {111}<112> at medium-temperature (760–1050 °C) and high-stress (270–810 MPa) creep, which brings a considerably low creep rate and high creep life to the Ru-containing alloy.
One-Pot Fabrication of Nanocomposites Composed of Carbon Nanotubes and Alumina Powder Using a Rotatable Chemical Vapor Deposition System
Authors: Lee Jong-Hwan , Han Hyun-Ho , Seo Jong-Min , Jeong Goo-Hwan
Published: March 2023

The fabrication of multi-dimensional nanocomposites has been extensively attempted to achieve synergistic performance through the uniform mixing of functional constituents. Herein, we report a one-pot fabrication of nanocomposites composed of carbon nanotubes (CNTs) and Al2O3 powder. Our strategy involves a synthesis of CNTs on the entire Al2O3 surface using a rotatable chemical vapor deposition system (RCVD). Ehylene and ferritin-induced nanoparticles were used as the carbon source and wet catalyst, respectively. The RCVD was composed of a quartz reaction tube, 5.08 cm in diameter and 150 cm in length, with a rotation speed controller. Ferritin dissolved in deionized water was uniformly dispersed on the Al2O3 surface and calcinated to obtain iron nanoparticles. The synthesis temperature, time, and rotation speed of the chamber were the main parameters used to investigate the growth behavior of CNTs. We found that the CNTs can be grown at least around 600 °C, and the number of tubes increases with increasing growth time. A faster rotation of the chamber allows for the uniform growth of CNT by the tip-growth mechanism. Our results are preliminary at present but show that the RCVD process is sufficient for the fabrication of powder-based nanocomposites.
Investigation of the Hot Stamping-in-Die Quenching Composite Forming Process of 5083 Aluminum Alloy Skin
Authors: Yi Lingling , Yu Ge , Tang Ziming , Li Xin , Gu Zhengwei
Published: March 2023

Aluminum alloy has been used as the skin material for rail vehicles and automobiles to meet the requirements of environmental protection. The hot stamping-in-die quenching composite forming (HFQ) process is a promising technology to compensate for the poor formability of the aluminum alloy sheet at room temperature. In this paper, the high-temperature mechanical properties of 5083 aluminum alloy under various temperature (200 °C, 300 °C, 400 °C, 450 °C) and strain rate conditions (0.01 s−1, 0.10 s−1, 1.00 s−1) were investigated by uniaxial tensile tests. The finite element software of PAM-STAMP was employed to simulate the forming process of high-speed train skin. The effects of forming method and process parameters on the minimum thickness and springback of the skin were analyzed using the Response Surface Methodology (RSM). After parameter optimization, the forming experiment verified the simulation results and the test part met the quality requirements: the thickness above 3.84 mm and the springback within 1.1 mm. Mechanical properties of the sheet before and after HFQ were examined by uniaxial tensile tests at room temperature. It can be inferred from the comparison that the yield strength of the Al5083 sheet increases, but the elongation decreases from the HFQ process.