bluuebunny/crossref_metadata_2025 huggingface dataset:
Integrated multiple-microarray analysis and mendelian randomization to identify novel targets involved in diabetic nephropathy
Authors: Fan Chenyu , Gao Yuye , Sun Ying
Published: July 2023

Background

Diabetic nephropathy (DN), which is the main cause of renal failure in end-stage renal disease, is becoming a common chronic renal disease worldwide. Mendelian randomization (MR) is a genetic tool that is widely used to minimize confounding and reverse causation when identifying the causal effects of complex traits. In this study, we conducted an integrated multiple microarray analysis and large-scale plasma proteome MR analysis to identify candidate biomarkers and evaluate the causal effects of prospective therapeutic targets in DN.

Methods

Five DN gene expression datasets were selected from the Gene Expression Omnibus. The robust rank aggregation (RRA) method was used to integrate differentially expressed genes (DEGs) of glomerular samples between patients with DN and controls, followed by functional enrichment analysis. Protein quantitative trait loci were incorporated from seven different proteomic genome-wide association studies, and genetic association data on DN were obtained from FinnGen (3676 cases and 283,456 controls) for two-sample MR analysis. External validation and clinical correlation were also conducted.

Results

A total of 82 DEGs (53 upregulated and 29 downregulated) were identified through RRA integrated analysis. The enriched Gene Ontology annotations and Kyoto Encyclopedia of Genes and Genomes pathways of the DEGs were significantly enriched in neutrophil degranulation, neutrophil activation, proteoglycan binding, collagen binding, secretory granule lumen, gluconeogenesis, tricarboxylic acid cycle, and pentose phosphate pathways. MR analysis revealed that the genetically predicted levels of MHC class I polypeptide-related sequence B (MICB), granzyme A (GZMA), cathepsin S (CTSS), chloride intracellular channel protein 5, and ficolin-1 (FCN1) were causally associated with DN risk. Expression validation and clinical correlation analysis showed that MICB, GZMA, FCN1, and insulin-like growth factor 1 may participate in the development of DN, and carbonic anhydrase 2 and lipoprotein lipase may play protective roles in patients with DN.

Conclusion

Our integrated analysis identified novel biomarkers, including MICB and GZMA, which may help further understand the complicated mechanisms of DN and identify new target pathways for intervention.
Influence of hydrothermal factors on a coniferous forest canopy in the semiarid alpine region of Northwest China
Authors: Zhao Yonghong , Zhao Weijun , Fang Huijun
Published: July 2023

Analyzing the physiological response of trees to climate change in the Qilian Mountains region is key to studying the impact of global change on forest ecosystems in the semiarid alpine region of Northwest China. The leaf area index (LAI) of the canopy of a forest is an important input parameter for simulating carbon and water cycles in forest ecosystems. Studying the relationship between LAI and environmental factors can provide a scientific basis for accurately describing the structure, function, and ecohydrological processes of forest ecosystems and theoretically guide for sustainable management of water conservation in forests. Methods: In this study, the LAI of the Picea crassifolia canopy was monitored for 2 years (2015–2016) by field observations, and its dynamic changes were analyzed. The relations between LAI and air temperature (AT), precipitation (P), soil temperature (ST), and soil water content (SWC) were studied using Pearson's correlation and multiple regression analyses. The results were as follows: seasonal variations in LAI showed a single-peak curve, which first increased, reached a maximum, remained relatively stable, and then decreased. The maximum value was 4.02 and 4.18 relatively observed in mid-August 2015 and 2016. The LAI of the P. crassifolia canopy in different months was positively correlated with AT and P. It was correlated between the LAI of the canopy with ST40−60 in May and June (p < 0.05) and was also highly positively correlated between the LAI of the canopy with ST60−80, STmean, and SWC60−80 in July and August (p < 0.01). There was a positive correlation between the LAI of the canopy with SWC0−60 and SWCmean in July and SWC0−60 and SWCmean in August (p < 0.05). The LAI of the canopy was affected by AT and ST in May and July, AT and P in June, P in August, and P and ST in September. Our study implied that the rapid increase period of the LAI of the canopy was from late May to early July. The LAI of the canopy was more influenced by temperature and water in July and August. In addition, the LAI of the canopy has significant seasonal variation although it is evergreen coniferous tree species.
Analysis of platelet and monocyte-to-lymphocyte ratio and diabetes mellitus with benign prostatic enlargement
Authors: Chen Guanheng , Feng Leiguang
Published: July 2023

Background

The etiology of benign prostatic hyperplasia (BPH) is still elusive. The aim of this study was to provide preventive and prognostic parameters associated with diabetes mellitus with benign prostatic enlargement (BPE).

Methods

Diabetic patients were collected retrospectively from February 2021 to December 2022, including monocyte-to-lymphocyte ratio (MLR). Diabetic patients were divided into two groups by whether the prostate volume was greater than or equal to 30 ml, which were diabetes mellitus without BPE (DM) and diabetes mellitus with BPE (DM+BPE). The baseline characteristics were compared, the risk and protective factors associated with DM+BPE were determined using univariate and multivariate logistic regression, and the parameters associated with prostate volume were determined using correlation analysis.

Results

Of the 671 patients collected, age and prostate volume were significantly higher in the DM+BPE than in the DM; MLR was higher in the DM+BPE than in the DM; and platelet was significantly lower in the DM+BPE than in the DM. Univariate logistic regression showed that age was a risk factor, while protective factors for DM+BPE were lymphocytes and platelet. Multifactorial logistic regression showed that age was a risk factor, while platelet was the protective factor for DM+BPE. In the total overall (n=671), prostate volume was positively correlated with age. Prostate volume was negatively correlated with lymphocytes and platelet. In DM+BPE (n=142), prostate volume was positively correlated with age and MLR.

Conclusion

Platelet was a protective factor for DM+BPE and was negatively correlated with prostate volume, whereas MLR was positively correlated with prostate volume in DM+BPE.
Transcriptome analysis reveals reduced immunity and metabolic level under cold stress in Mauremys mutica
Authors: OuYang Jiahui , Gao Yangchun , Wei Yufeng , Huang Huangping , Ge Yan , Zhao Jun , Gong Shiping
Published: July 2023

The yellow pond turtle (Mauremys mutica) is widely cultured for food, medicine and for keeping as pets in China. The high risk of disease outbreaks and mortality caused by sudden sharp temperature drop events has significant negative effects on the aquaculture industry of M. mutica. However, the mechanism underlying the damage caused by cold stress is still unclear. To fill this gap, we performed transcriptome sequencing of M. mutica samples collected at three sampling time points (3h, 24h and 48h) during cold treatment, and at a recovery time point (96 h after the end of cold stress). The results showed that immunity and metabolism-related pathways (neuroactive ligand-receptor interactions, cytokine-cytokine receptor interactions and NOD-like receptor signaling pathways) were significantly enriched at both 24h and 48h. The expression of immunity and metabolism-related genes (BS2A1, PIWIL2 and Fads1) significantly decreased at all-time points compared to the control group. These results suggested that impaired immunity and depressed metabolism under cold stress may be the main cause of the massive cold-stress mortality of M. mutica. This study provides novel insights into the molecular mechanisms underlying mass disease and mortality caused by sudden sharp temperature drop events in M. mutica.
Mixed oligosaccharides-induced changes in bacterial assembly during cucumber (Cucumis sativus L.) growth
Authors: Wang Qiushui , Zhou Xin , Liu Yue , Han Yan , Zuo Jia , Deng Jie , Yuan Liyan , Gao Lijuan , Bai Wenbo
Published: July 2023

The application of oligosaccharides can promote plant growth by increasing photosynthesis or inducing plant innate immunity. However, the mechanisms by which oligosaccharides affect bacterial community diversity and abundance remain unclear. In this study, a mixed oligosaccharide was applied to the growth of cucumbers. The findings of the present study suggest that the application of MixOS has significant effects on the bacterial communities in the phyllosphere, rhizosphere, and bulk soil of cucumber plants. The treatment with MixOS resulted in delayed senescence of leaves, well-developed roots, and higher fruit production. The bacterial diversity and composition varied among the different ecological niches, and MixOS application caused significant shifts in the bacterial microbiome composition, particularly in the phyllosphere. Moreover, mixed oligosaccharides increased the abundance of potential growth-promoting bacteria such as Methylorubrum spp. and Lechevalieria spp., and more zOTUs were shared between the WM and MixOS treatments. Furthermore, the bacterial co-occurrence network analysis suggested that the modularity of the phyllosphere networks was the highest among all samples. The bacterial co-occurrence networks were altered because of the application of MixOS, indicating a greater complexity of the bacterial interactions in the rhizosphere and bulk soil. These findings suggest that mixed oligosaccharides has the potential to improve plant growth and yield by modulating the bacterial communities within and outside the plants and could provide a theoretical basis for future agricultural production.
Predicting the severity of white matter lesions among patients with cerebrovascular risk factors based on retinal images and clinical laboratory data: a deep learning study
Authors: Shu Liming , Zhong Kaiyi , Chen Nanya , Gu Wenxin , Shang Wenjing , Liang Jiahui , Ren Jiangtao , Hong Hua
Published: July 2023

Background and purpose

As one common feature of cerebral small vascular disease (cSVD), white matter lesions (WMLs) could lead to reduction in brain function. Using a convenient, cheap, and non-intrusive method to detect WMLs could substantially benefit to patient management in the community screening, especially in the settings of availability or contraindication of magnetic resonance imaging (MRI). Therefore, this study aimed to develop a useful model to incorporate clinical laboratory data and retinal images using deep learning models to predict the severity of WMLs.

Methods

Two hundred fifty-nine patients with any kind of neurological diseases were enrolled in our study. Demographic data, retinal images, MRI, and laboratory data were collected for the patients. The patients were assigned to the absent/mild and moderate–severe WMLs groups according to Fazekas scoring system. Retinal images were acquired by fundus photography. A ResNet deep learning framework was used to analyze the retinal images. A clinical-laboratory signature was generated from laboratory data. Two prediction models, a combined model including demographic data, the clinical-laboratory signature, and the retinal images and a clinical model including only demographic data and the clinical-laboratory signature, were developed to predict the severity of WMLs.

Results

Approximately one-quarter of the patients (25.6%) had moderate–severe WMLs. The left and right retinal images predicted moderate–severe WMLs with area under the curves (AUCs) of 0.73 and 0.94. The clinical-laboratory signature predicted moderate–severe WMLs with an AUC of 0.73. The combined model showed good performance in predicting moderate–severe WMLs with an AUC of 0.95, while the clinical model predicted moderate–severe WMLs with an AUC of 0.78.

Conclusion

Combined with retinal images from conventional fundus photography and clinical laboratory data are reliable and convenient approach to predict the severity of WMLs and are helpful for the management and follow-up of WMLs patients.
Characteristics of EEG power spectra involved in the proficiency of motor learning
Authors: Hamada Hiroyuki , Wen Wen , Kawasaki Tsubasa , Yamashita Atsushi , Asama Hajime
Published: July 2023

Neuromodulation techniques for modulating brain activity can affect performance in a variety of behaviors. Techniques including transcranial alternating current stimulation and random noise stimulation can modulate neural oscillations. However, the intervention effect of neuromodulation approaches on motor learning is poor, partly because the electroencephalography (EEG) power spectra associated with the motor learning process has not yet been fully elucidated. Therefore, we investigated the characteristics of EEG power spectra in the process of motor learning in 15 right-handed healthy participants (5 females; mean age = 22.8 ± 3.0 years). The motor task was a ball-rotation task in which participants rotated two balls in the palm of their left hand. Participants performed a pre-test, the motor learning tasks, and a post-test. In the motor learning tasks, twenty 60 s trials were performed in the clockwise (CW) direction. Before and after the motor learning tasks, CW and counterclockwise (CCW; control condition) tasks were performed for 60 s each as pre- and post-tests. Therefore, CW direction was set as a motor learning task, while CCW was a test-only control task. EEG was recorded during the tests and tasks, and the power spectra in the alpha, beta, and gamma oscillations were calculated and compared between pre- and post-tests. The results showed that in the CW post-test, the power of the gamma band in the left parietal areas and the right frontal area was significantly higher than in the pre-test. In the CCW, there was no significant difference in each band at each area between the pre- and post-tests. Our findings reveal the characteristics of the EEG spectra related to the motor learning process. These results may help to establish more effective neuromodulation approaches to modifying neural oscillations in motor learning, including in rehabilitation fields.
Publication trends and hotspots of colorectal adenoma during 2002-2022: a bibliometric and visualized analysis
Authors: Li Xue , Zhang Wenzheng , Yuan Surui , Mao Qiyuan , Zhang Chuchu , Cai Ruijuan , Lin Hongsheng , Wang Xueqian
Published: July 2023

Background

Prevention and treatment of colorectal adenoma (CRA) are great significant to reduce morbidity and mortality of colorectal cancer. Although there have been numerous studies on CRA recently, few publications utilized the bibliometrics to evaluate this field. The objective of current study was to provide a comprehensive analysis of the current state and frontier progress of CRA over the past 20 years.

Methods

The Web of Science Core Collection was utilized to extracted all studies of CRA during 2002-2022. Bibliometric tools including CiteSpace, VOSviewer, and the Online Analysis Platform of Literature Metrology were used for statistical analysis. CiteSpace and the Online Analysis Platform were used to evaluate the contributions of various countries/regions, institutions, authors, and journals in this field. Research hotspots and trends were identified through keywords and references analysis by VOSviewer and CiteSpace.

Results

2,268 publications from 2002 to 2022 in total were identified. The number of global publications in this field has increased annually. The USA was the most productive country, contributing nearly 30% of global publications. But in recent years, China’s publications grew rapidly and had the highest citation strength. The most productive institutions was the National Cancer Institute. Baron JA from the USA was the most productive and the one of most co-cited authors. Cancer Epidemiology Biomarkers & Prevention had the highest number of publications and Gastroenterology was the most co-cited journals. Analysis of keywords clusters showed that “mechanism/pathophysiology”, “risk factors and prevention”, “colonoscopy screening and treatment”, “metabolism”, and “microbiota” were the major frontier topics and the main research directions.

Conclusions

CRA publications have shown a gradual upward trend in recent years, most of which have been published by developed countries. Developing countries should further focus on CRA research and transnational cooperation with developed countries in the future, in order to better improve the situation of the increasing morbidity and mortality of CRC. Baron JA was the most outstanding researcher in this field. More attention should be devoted to “pathogenesis of CRA”, “less invasive diagnostic methods”, “chemoprevention”, and “screening and risk prediction of CRA including gut microbiome and metabolism”, which will be frontiers in the future.
Small bowel edema and lymphocytic duodenitis as severe reversible gastrointestinal toxicity of selpercatinib in RET fusion–positive non–small cell lung cancer: a case report
Authors: Scattolin Daniela , Scagliori Elena , Scapinello Antonio , Fantin Alberto , Guarneri Valentina , Pasello Giulia
Published: July 2023

Introduction

Rearranged during transfection (RET) gene rearrangements occur in 1%–2% of non–small cell lung cancer (NSCLC). Because of the results of the study LIBRETTO-001, selpercatinib has been approved as the first-line treatment for patients with RET fusion–positive advanced NSCLC. Selpercatinib demonstrated to be well tolerated. Despite this, gastrointestinal adverse events (AEs) are frequently reported, and no clinical-radiological and endoscopic features and their impact in terms of treatment discontinuations, interruptions, and dose reductions have been described so far.

Case report

A 37-year-old never-smoker woman was treated in our institution with selpercatinib for a RET fusion–positive NSCLC. After 9 months of treatment, the patient referred abdominal pain of grade (G) 2, associated with nausea of G2, bilious vomiting of G3, and weight loss of G1. At computed tomography scan, the presence of important bowel wall thickening, free ascitic fluid, mesenteric congestion, and stranding was detected. The patient underwent an anterograde enteroscopy extended to jejunum with detection of lymphocytic duodenitis with sub-mucosal edema. Selpercatinib treatment was temporary interrupted with complete resolution of the symptoms and then re-administered with dose reduction, without relapsed of the gastrointestinal toxicity after 120 days.

Conclusion

To our knowledge, this is the first case report of a patient with NSCLC treated with selpercatinib outside a clinical study who developed severe gastrointestinal toxicity characterized by small bowel edema and lymphocytic duodenitis, leading to treatment interruption and dose reduction. The gastrointestinal AE has been described by a radiological, endoscopic, and histopathological point of view. Further investigations are needed to better identify pathological mechanisms of gastrointestinal toxicity for an appropriate AE management.
Development of a vaccine against the synthetic opioid U-47700
Authors: Park Hyeri , Lin Mingliang , Zhou Jian , Eubanks Lisa M. , Zhou Bin , Janda Kim D.
Published: July 2023

Opioid use disorders and overdose have become a major public health concern in recent years. U-47700, a New psychoactive substances (NPS) opioid, also known as “pinky” or “pink” has been identified as a new threat in the drug supply because of its potency and abuse potential. Conjugate vaccines that can produce antibodies against target drug molecules have emerged as a promising tool to treat substance use disorders. Herein, we report the design, synthesis, and in vivo characterization of a U-47700 vaccine. The vaccine demonstrated favorable results with rodents producing elevated levels of antibody titer and sub-micromolar affinity to U-47700. In addition, antibodies generated by the vaccine effectively mitigated drug-induced effects by preventing the drug from penetrating the blood-brain barrier, which was verified by antinociception and drug biodistribution studies. The development of a vaccine against U-47700 and other NPS opioids contributes to the continued advancement of non-conventional pharmacological treatments to address the global opioid epidemic.
Infrared defect recognition technology for composite materials
Authors: Chang Hao-Liang , Ren Hao-Tian , Wang Gang , Yang Ming , Zhu Xin-Yu
Published: July 2023

This study mainly involves the methods and experiments of using infrared thermal wave imaging detection technology to detect internal defects in aircraft composite materials. The results were discussed and analyzed. In this paper, the feasibility of the experiment was verified by simulation. In simulation, the minimum accuracy of detectable defects is 4 mm radius under the mesh division accuracy with a correlation coefficient of 5. On this basis, an accurate detection method and prototype nondestructive testing system for defects of aircraft composite materials based on infrared imaging detection technology were designed, which can realize the identification and positioning of defects in aircraft composite material structures, including type, size and accurate depth of defects. Finally, the data collected by the infrared detection system was recognized through YOLO neural network. The test result shows the confidence level for water point defect is more than 0.9, while the confidence level for crack defect is about 0.5. This research result will expand the use case of infrared nondestructive testing technology around the world, and the transformation of the results will help to solve the maintenance problems of aircraft in general aviation.
Apoptotic volume decrease (AVD) in A549 cells exposed to water-soluble fraction of particulate matter (PM10)
Authors: Giordano M. E. , Udayan G. , Guascito M. R. , De Bartolomeo A. R. , Carlino A. , Conte M. , Contini D. , Lionetto M. G.
Published: July 2023

Exposure to atmospheric particulate matter (PM) is recognized as a human health risk factor of great concern. The present work aimed to study the cellular mechanisms underlying cytotoxic effects of airborne particulate matter <10 µm in size (PM10), sampled in an urban background site from January to May 2020, on A549 cells. In particular, the study addressed if PM10 exposure can be a main factor in the induction of the Apoptotic Volume Decrease (AVD), which is one of the first events of apoptosis, and if the generation of intracellular oxidative stress can be involved in the PM10 induction of apoptosis in A549 cells. The cytotoxicity of PM10 samples was measured by MTT test on cells exposed for 24 h to the PM10 aqueous extracts, cell volume changes were monitored by morphometric analysis of the cells, apoptosis appearance was detected by annexin V and the induction of intracellular oxidative stress was evaluated by the ROS sensitive CM-H2DCFDA fluorescent probe. The results showed cytotoxic effects ascribable to apoptotic death in A549 cells exposed for 24 h to aqueous extracts of airborne winter PM10 samples characterized by high PM10 value and organic carbon content. The detected reduced cell viability in winter samples ranged from 55% to 100%. Normotonic cell volume reduction (ranging from about 60% to 30% cell volume decrease) after PM10 exposure was already detectable after the first 30 min clearly indicating the ability of PM10, mainly arising from biomass burning, to induce Apoptotic Volume Decrease (AVD) in A549 cells. AVD was prevented by the pre-treatment with 0.5 mM SITS indicating the activation of Cl− efflux presumably through the activation of VRAC channels. The exposure of A549 cells to PM10 aqueous extracts was able to induce intracellular oxidative stress detected by using the ROS-sensitive probe CM-H2DCFDA. The PM10-induced oxidative stress was statistically significantly correlated with cell viability inhibition and with apoptotic cell shrinkage. It was already evident after 15 min exposure representing one of the first cellular effects caused by PM exposure. This result suggests the role of oxidative stress in the PM10 induction of AVD as one of the first steps in cytotoxicity.
The relationship between the Dark Triad and bullying among Chinese adolescents: the role of social exclusion and sense of control
Authors: Huang Yongqi , Gan Xiong , Jin Xin , Rao Shijie , Guo Binbin , He Zijian , Wei Zixu
Published: July 2023

Introduction

Abundant evidence has proved the association between the Dark Triad and bullying. However, the underlying mechanisms of this relationship are still not fully understood. Based on the temporal need-threat model, three studies were designed to explore the mediating role of social exclusion and sense of control in this research.

Methods

In study 1 we recruited 571 Chinese adolescents (Mage = 14.53, SD = 0.716) to participate in a cross-sectional study. And two experiments were respectively designed in Study 2 (N = 88) and Study 3 (N = 102) to verify the effects of real and cyber social exclusion on adolescent bullying behavior.

Results

Study 1 showed that social exclusion and sense of control would play the serial mediating role in the relationship between the Dark Triad and bullying (except social exclusion as a mediator between the Dark Triad and cyberbullying). Study 2 and 3 showed that adolescents with high Dark Triad show lower sense of control and more bullying behavior after experiencing social exclusion.

Discussion

These findings extend the research on the Dark Triad and bullying by providing a solid empirical foundation and intervention strategies to avoid bullying so that the problem can be rationally and scientifically approached.
Mental health treatment and access for emerging adults in Canada: a systematic review
Authors: Murray Jonah Kynan , Knudson Sarah
Published: July 2023

Introduction

Research into the mental healthcare of emerging adults (18–25) in Canada has been limited, despite this developmental period being widely considered a vulnerable time of life. As such, we aimed to identify the greatest barriers emerging adults faced in accessing mental healthcare in Canada, particularly in relation to the Canadian healthcare system which operates on a universal funding model but is challenged by funding shortfalls and a complex relationship to the provinces.

Methods

We systematically examined 28 pieces of literature, including academic and technical literature and publications from government organizations, focused on emerging adults and the Canadian mental healthcare system.

Results

Findings demonstrated that stigma, a lack of mental health knowledge, cost, and interpersonal factors (e.g., one’s parental, peer, and romantic supports demonstrating negative views toward mental healthcare may deter treatment; emerging adults demonstrating concerns that accessing mental healthcare may lead to peer rejection) acted as barriers to help-seeking in emerging adults. Additionally, a lack of national institutional cohesion and a lack of policy pertaining to emerging adult healthcare acted as barriers to adequate mental healthcare in this demographic.

Discussion

Improving mental health education early in life shows promise at reducing many of the barriers emerging adults face in accessing mental healthcare. Further, policies directed at ensuring a cohesive national mental health system, as well as policies directly designed to care for emerging adult mental health needs, could act as the next steps toward ensuring an accessible and effective Canadian mental healthcare system that can serve as a model for other nations.
Adherence to 24-h movement guidelines in Spanish schoolchildren and its association with insulin resistance: a cross-sectional study
Authors: Salas-González María Dolores , Bermejo Laura M. , González-Rodríguez Liliana G. , Lozano-Estevan María Del Carmen , Ortega Rosa M. , López-Sobaler Ana M.
Published: July 2023

Introduction

Being more active, being less sedentary, and sleeping enough are associated with adequate body weight and adiposity in children. However, few researchers have analyzed these different lifestyle behaviors and the adherence to 24 h movement guidelines with respect to insulin resistance (IR) at school age. Therefore, we aimed to analyse the association between the adherence to 24 h movement guidelines (physical activity, sedentary time, sleep) and IR in Spanish schoolchildren.

Methods

A cross-sectional study of 839 children (8–13 years, 51.1% girls) were studied. Anthropometric, biochemical, and lifestyle behavioral data were collected. IR was defined as HOMA-IR>3.16. Compliance with the 24 h movement guidelines were defined as ≥60 min/day of moderate and/or vigorous physical activity, < 120 min/day of screen time, and 9–11 h/day of sleep time. Associations between adherence to the 24 h movement guidelines and IR were performed by multivariate logistic regression analyses.

Results

The IR in our sample was 5.0%, being higher in girls. Compliance with physical activity or screen time, but not with sleep recommendations, was associated with lower fasting glucose, fasting insulin, and HOMA-IR values. A low adherence to 24 h movement guidelines was associated with a higher risk of IR (odds ratio (95% CI): 2.150 [1.089–4.247]), especially in girls (odds ratio (95% CI): 2.800 [1.180–6.645]).

Conclusion

Higher physical activity levels and lower screen times were associated with a lower risk of IR in schoolchildren, underlining the importance of adhering to as many healthy lifestyle recommendations as possible.
Household food insecurity levels in Ethiopia: quantile regression approach
Authors: Wubetie Habtamu T. , Zewotir Temesgen , Mitku Aweke A. , Dessie Zelalem G.
Published: July 2023

Introduction

Numerous natural and man-made factors have afflicted Ethiopia, and millions of people have experienced food insecurity. The current cut-points of the WFP food consumption score (FCS) have limitations in measuring the food insecurity level of different feeding patterns due to the diversified culture of the society. The aim of this study is to adapt the WFP food security score cut-points corrected for the different feeding cultures of the society using effect-driven quantile clustering.

Method

The 2012, 2014, and 2016 Ethiopian socio-economic household-based panel data set with a sample size of 3,835 households and 42 variables were used. Longitudinal quantile regression with fixed individual-specific location-shift intercept of the free distribution covariance structure was adopted to identify major indicators that can cluster and level quantiles of the FCS.

Result

Household food insecurity is reduced through time across the quintiles of food security score distribution, mainly in the upper quantiles. The leveling based on effect-driven quantile clustering brings 35.5 and 49 as the FCS cut-points corrected for cultural diversity. This corrected FCS brings wider interval for food insecure households with the same interval range for vulnerable households, where the WFP FCS cut-points under estimate it by 7 score. Education level, employment, fertilizer usage, farming type, agricultural package, infrastructure-related factors, and environmental factors are found to be the significant contributing factors to food security. On the other hand, the age of the head of the household, dependency ratio, shock, and no irrigation in households make significant contributions to food insecurity. Moreover, households living in rural areas and farming crops on small lands are comparatively vulnerable and food insecure.

Conclusion

Measuring food insecurity in Ethiopia using the WFP FCS cut-off points underestimates households’ food insecurity levels. Since the WFP FCS cut-points have universality and comparability limitations, there is a need for a universally accepted local threshold, corrected for local factors those resulted in different consumption patterns in the standardization of food security score. Accordingly, the quantile regression approach adjusts the WFP-FCS cut points by adjusting for local situations. Applying WFP cut-points will wrongly assign households on each level, so the proportion of households will be inflated for the security level and underestimated for the insecure level, and the influence of factors can also be wrongly recommended the food security score for the levels. The quantile clustering approach showed that cropping on a small land size would not bring about food security in Ethiopia. This favors the Ethiopian government initiative called integrated farming “ኩታ ገጠም እርሻ” which Ethiopia needs to develop and implement a system that fits and responds to this technology and infrastructure.
Knowledge, attitudes, and practices toward COVID-19 prevention in Yemen: a community-based cross-sectional study
Authors: Hassan Ola El Hajj , Al-Aghbari Ahmed Asa’ad , McGowan Maureen , Dar Iang Maureen , Basaleem Huda Omer , Al-Sakkaf Khaled Abdulla , Hyzam Dalia , Jahn Albrecht , Dureab Fekri
Published: July 2023

Background

Pandemics, especially in fragile war-torn countries like Yemen, challenge their already strained health systems. Community adherence to pandemic prevention measures is necessary to curb the severity and spread of emerging pandemics – which is influenced by factors, such as people’s knowledge and attitudes toward the pandemic. No studies in Aden have been published on the communities’ knowledge, attitudes, and practices (KAP) toward COVID-19 prevention to date. To understand adherence to pandemic prevention measures in contexts with fragile health systems, this study investigated KAP of Yemeni participants toward the COVID-19 pandemic.

Methods

We conducted face-to-face semi-structured questionnaires among 400 eligible participants whom were identified for participation in this study through systematic household sampling from eight districts in Aden, Yemen. Eligible participants were Yemeni community members who were ≥ 18 years, living for more than 10 years in Yemen, and were willing to voluntarily participate in the study. The questionnaire included questions surrounding the participants’ COVID-19 knowledge (e.g., awareness of spread and prevention), attitudes (e.g., willingness to accept the vaccine or other prevention measures), and prevention practices during the pandemic (e.g., mask wearing, social distancing, vaccine uptake). Total KAP scores were calculated. Univariate and bivariate statistical analyses were conducted using STATA 13 software.

Results

From January to May 2021 we conducted 400 questionnaires with Yemeni community members. The average age was 41.5 ± 14.5 years (range 18–86 years). The results demonstrated that the participants in this study had an intermediate knowledge (53%) and fair attitude (58%) scores. However, participants reported very poor COVID-19 prevention practices- with only 11% demonstrating these practices. Only 25% (100/400) practiced social distancing, 25% (98/400) wore a mask, and only 6% (27/400) of participants accepted (at least one dose of) the COVID-19 vaccine. Factors associated with increased knowledge were being male, married, and surprisingly those having a primary and middle school education levels (p < 0.05). Also participants who were diagnosed with COVID-19 or had a family member diagnosed with COVID-19 (vs. those not diagnosed OR = 2.08, 95% CI 1.07–3.78, p < 0.05) were more likely to know that the vaccine protects against severe COVID-19 infection and were more likely to apply good practices such as accepting the vaccine (OR = 2.65, 95% CI 1.17–6.00, p < 0.05) compared to those who were not.

Conclusion

These findings raise awareness for the need of community-oriented education programs for COVID-19 which considers associated factors to improve the level of public knowledge, attitudes, and practices.
Clinical impact of early post-transplant red cell transfusions in kidney transplantation: a systematic review and meta-analysis
Authors: Hassan Sevda , Gleeson Sarah , Thomson Tina , Spensley Katrina J. , Dor Frank , Brown Colin , Regan Fiona , Pengel Liset H. M. , Willicombe Michelle , Roberts David J.
Published: July 2023

Introduction

Red blood cell transfusions (RBCT) represent a potentially modifiable risk factor for HLA sensitisation and adverse outcomes post transplantation. Evidence of the clinical impact of post-transplant RBCT has been infrequently reported. Herein, we performed a systematic review of available literature to assess the prevalence of RBCT post kidney transplant, and the effect of transfusion on transplant outcomes.

Methods

We included studies from 2000 to July 2022, published on Medline, Embase and the Transplant Library.

Results

Ten studies were analysed which included a total of 32,817 kidney transplant recipients, with a median transfusion prevalence of 40% (range 18-64%). There was significant heterogeneity between studies in terms of patient and allograft characteristics, immunological risk, and immunosuppression protocols. Analysis of unadjusted outcomes showed that post-transplant RBCTs are associated with inferior patient survival, allograft loss, rejection and donor specific antibodies. Adjusted outcomes were described where available, and supported the adverse associations seen in the unadjusted models in many studies.

Discussion

This review demonstrates that RBCT post-transplant are common and maybe associated with inferior outcomes, highlighting the urgent need for high quality prospective evidence of the effect of RBCTs on transplant outcomes.

Systematic Review Registration

https://www.crd.york.ac.uk/prospero/, identifier, CRD42022348763767.
Model Analysis of Steel Frame Structures Considering Interactions between Racks and the Frame
Authors: Zhang Weiguang , Yu Chaoqun , Tong Genshu
Published: July 2023

The steel racks on the floor are seen as live loads in the current design process, ignoring the interaction with the supporting frames. In this paper, multiple steel racks with different masses and stiffnesses are placed on the first floor of a two-story main structure to form different real structures (RS). The corresponding simplified structures (SS) are frames with the mass of steel racks concentrated on the first floor of the main structure. Modal analysis is performed to analyze the relationship between the periods of RS and SS in the cross-aisle direction. Firstly, the beams on the first floor are assumed to be infinitely rigid. The relationship between the periods of the rack TRk, the simplified structure TSS, and the real structure TRS under different mass ratios α is established, and an accurate equation relating TRS with TRk and TSS is proposed. Moreover, by considering the influence of finite beam stiffness, the interaction between racks and the main structure is studied by constructing different analysis models. The effect of the main structure on the racks is reflected by a combined system consisting of beams and racks. A modified model, distinguished from SS by considering the effect of no-mass racks, is constructed to study the strengthening effect of the racks on the first-floor beams. The effect of the top connecting bars is also analyzed.
Divergent Responses of Tomato Cultivars with Resistance to Tomato Yellow Leaf Curl Virus as Infected by Meloidogyne javanica
Authors: Verdejo-Lucas Soledad
Published: July 2023

Commercial tomatoes are usually complex F1 hybrids with multiple resistances genes from different wild Solanum species. The response of tomato cultivars with resistance to root-knot nematodes (RKN) and Tomato yellow leaf curl virus (TYLCV) as infected by Meloidogyne javanica was determined in a controlled environment and field conditions. Four treatments were tested, viz. tomato cultivars with (i) RKN resistance alone; (ii) combination of RKN and TYLCV resistance (RKN + TYLCV); (iii) TYLCV resistance alone; and (iv) control (susceptible to the nematode and virus). The RKN-resistant plants effectively suppressed nematode infection and reproduction both in a controlled environment and in field conditions. The RKN + TYLC-resistant plants were less effective (p < 0.001) than the RKN plants in a controlled environment, and their resistance levels were significantly reduced in the field. Nonetheless, the RKN + TYLCV plants supported lower (p < 0.001) nematode infection and reproduction than the susceptible control plants. The TYLCV-resistant plants reduced (p < 0.001) nematode infection and reproduction compared to the susceptible control in a controlled environment and in field conditions. The divergent response of tomato cultivars with resistance to TYLCV via infection by M. javanica can be attributed to the genetic background of the cultivars.
Comparison of Prognostic Performance between Procalcitonin and Procalcitonin-to-Albumin Ratio in Post Cardiac Arrest Syndrome
Authors: Yoon Ju Hee , Choi Woo Sung , Lim Yong Su , Jang Jae Ho
Published: July 2023

(1) Background: Post-cardiac arrest syndrome (PCAS) is a type of global ischemic reperfusion injury that occurs after the return of spontaneous circulation (ROSC). The procalcitonin to albumin ratio (PAR) has been studied as an independent prognostic factor of various diseases. There are no previous studies of PAR in patients with PCAS. We assessed if PAR is more effective than procalcitonin (PCT) in predicting prognosis for patients with PCAS. (2) Methods: This retrospective cohort study included a total of 187 patients with PCAS after non-traumatic out-of-hospital cardiac arrest (OHCA) between January 2016 and December 2020. Multivariate logistic regression analysis was conducted to assess the association between PAR and PCAS prognosis. The predictive performance of PAR was compared with PCT via the receiver-operating characteristic (ROC) analysis and DeLong test.; (3) Results: PAR at 24 and 48 h after hospital admission were independently associated with one-month neurological outcome (OR: 1.167, 95% CI: 1.023–1.330; OR: 1.077, 95% CI: 1.012–1.146, p < 0.05). By ROC analysis, PAR showed better performance over PCT at 48 h after admission in predicting one-month CPC (0.763 vs. 0.772, p = 0.010). (4) Conclusions: Our findings suggest that PAR at 48 h after admission is more effective in predicting a one-month neurological outcome than PCT at 48 h after admission in patients with PCAS after OHCA.
Thermal Nanoimprint Lithography—A Review of the Process, Mold Fabrication, and Material
Authors: Unno Noriyuki , Mäkelä Tapio
Published: July 2023

Micro- and nanopatterns perform unique functions and have attracted attention in various industrial fields, such as electronic devices, microfluidics, biotechnology, optics, sensors, and smart and anti-adhesion surfaces. To put fine-patterned products to practical use, low-cost patterning technology is necessary. Nanoimprint lithography (NIL) is a promising technique for high-throughput nanopattern fabrication. In particular, thermal nanoimprint lithography (T-NIL) has the advantage of employing flexible materials and eliminating chemicals and solvents. Moreover, T-NIL is particularly suitable for compostable and recyclable materials, especially when applying biobased materials for use in optics and electronics. These attributes make T-NIL an eco-friendly process. However, the processing time of normal T-NIL is longer than that of ultraviolet (UV) NIL using a UV-curable resin because the T-NIL process requires heating and cooling time. Therefore, many studies focus on improving the throughput of T-NIL. Specifically, a T-NIL process based on a roll-to-roll web system shows promise for next-generation nanopatterning techniques because it enables large-area applications with the capability to process webs several meters in width. In this review, the T-NIL process, roll mold fabrication techniques, and various materials are introduced. Moreover, metal pattern transfer techniques using a combination of nanotransfer printing, T-NIL, and a reverse offset are introduced.
Euscaphis japonica Kanitz Fruit Exerts Antiobesity Effects by Inhibiting the Early Stage of Adipogenic Differentiation
Authors: Lee Eunbi , Park Juhye , Nam Ju-Ock
Published: July 2023

During the worldwide COVID-19 outbreak, there was an increase in the prevalence of obesity, including childhood obesity, due to which the awareness of obesity and interest in treatment increased. Accordingly, we describe EJF (Euscaphis japonica Kanitz fruit) extract as a candidate for naturally derived antiobesity agents. In this study, we found that EJF is involved in the early stage of adipogenic differentiation in vitro and finally inhibits adipogenesis. We propose two mechanisms for the antiobesity effect of EJF. First, EJF inhibits MDI-induced mitotic clonal expansion (MCE) by inducing cell cycle arrest at the initiation of adipogenic differentiation. The second aims to regulate stability and activation at the protein level of IRS1, which initiates differentiation in the early stage of differentiation. As a result, it was found that the activation of Akt decreased, leading to the inhibition of the expression of adipogenesis-related transcription factors (PPARγ, C/EBPα) and the subsequent suppression of adipogenic differentiation. In summary, we suggest that EJF can inhibit adipogenesis and lipid accumulation by suppressing the early stage of adipogenic differentiation in 3T3-L1 adipocytes. These findings indicate that EJF’s functionality could be beneficial in the treatment of obesity, particularly childhood obesity associated with adipocyte hyperplasia.
Urban Shrinkage and Labor Investment Efficiency: Evidence from China
Authors: Fang Hongru , Li Ran , Li Wenxing
Published: July 2023

In the context of unsustainable urban growth patterns and employment difficulties, we examine the impact of urban shrinkage on labor investment efficiency by drawing on the idea of the difference-in-difference model. Using a sample of Chinese firms from 2010 to 2019, our findings suggest that the widespread occurrence of urban shrinkage in China was responsible for a significant portion of the excessive labor investment in most listed companies. These results are robust to alternative specifications. In addition, we further found heterogeneity in the impact of urban shrinkage on labor investment efficiency. For state-owned or mature listed companies, this impact is manifested as the promotion of employment redundancy, while for non-state-owned or young companies, the impact is reflected in alleviating insufficient employment. Thus, we creatively identify a potential channel through which imbalanced development between cities affects corporate and economic sustainability.
Artists Profiles
Authors: Callander Adrienne , Taylor Johanna K. , Hernández Acosta Javier J.
Published: July 2023

Maniobra is a cultural employment initiative providing guaranteed income and additional support for artists and collectives in Puerto Rico over three years. The initiative is profiled in an article in this issue of Artivate by Javier J. Hernández Acosta, who is involved as both an arts entrepreneur launching the program through the Centro de Economía Creativa and as a scholar. A selection of the arts programs operating with Maniobra support is profiled here alongside the artists and collectives leading the work.