bluuebunny/crossref_metadata_2025 huggingface dataset:
Process and Mechanism of Exhumation in the Southern Altai Mountains, Northwest China
Authors: Li Shiyu , Yuan Wanming , Zhao Zhidan , Zhang Aikui , Dong Guochen , Li Xiaowei , Sun Wenli
Published: December 2024

This study presents new fission track data from 40 apatite and 40 zircon samples in the Southern Altai Mountains (SAMs), revealing apatite fission track (AFT) ages of 110 ± 8 Ma to 54 ± 4 Ma and zircon fission track (ZFT) ages of 234 ± 24 Ma to 86 ± 7 Ma. The exhumation rates derived from three thermochronological methods range from 0.01 to 0.1 km/Ma (Age-Elevation method), 0.01 to 0.14 km/Ma (Half-Space thermal model), and 0.027 to 0.075 km/Ma (Age2exhume model). Thermal history modeling using HeFTy software reveals similar thermal histories on both sides of the Kangbutiebao Fault, with a notable cooling event and higher exhumation rates to the northeast. The Late Cretaceous (100–75 Ma) rapid cooling is associated with tectonic reactivation, likely linked to the collapse of the Mongol–Okhotsk Orogen and slab rollback in the southern Tethys Ocean. In the Late Cenozoic (10–0 Ma), cooling and uplift reflect the influence of tectonic stresses from the India–Eurasia collision, which also drove the reactivation of the Kangbutiebao Fault. These findings suggest a complex interplay of tectonic processes driving exhumation in the SAMs from the Late Jurassic to the Early Paleogene.
Microwave-Assisted Production of Defibrillated Lignocelluloses from Blackcurrant Pomace via Citric Acid and Acid-Free Conditions
Authors: Inthalaeng Natthamon , Barker Ryan E. , Dugmore Tom I. J. , Matharu Avtar S.
Published: December 2024

Blackcurrant pomace (BCP) is an example of an annual, high-volume, under-utilized renewable resource with potential to generate chemicals, materials and bioenergy within the context of a zero-waste biorefinery. Herein, the microwave-assisted isolation, characterization and potential application of defibrillated lignocelluloses from depectinated blackcurrant pomace are reported. Depectination was achieved using citric acid (0.2–0.8 M, 80 °C, 2 h, conventional heating) and compared with acid-free hydrothermal microwave-assisted processing (1500 W, 100–160 °C, 30 min). The resultant depectinated residues were subjected to microwave-assisted hydrothermal defibrillation to afford two classes of materials: namely, (i) hydrothermal acid-free microwave-assisted (1500 W, 160 °C, 30 min; DFC-M1-M4), and (ii) hydrothermal citric acid microwave-assisted (1500 W, 160 °C, 30 min; DFC-C1–C4). Thermogravimetric analysis (TGA) revealed that the thermal stability with respect to native BCP (Td = 330 °C) was higher for DFC-M1-M4 (Td = 345–348 °C) and lower for DFC-C1–C4 (322–325 °C). Both classes of material showed good propensity to hold water but failed to form stable hydrogels (5–7.5 wt% in water) unless they underwent bleaching which removed residual lignin and hemicellulosic matter, as evidenced by 13C solid-state NMR spectroscopy. The hydrogels made from bleached DFC-C1–C4 (7.5 wt%) and bleached DFC-M1-M4 (5 wt%) exhibited rheological viscoelastic, shear thinning, and time-dependent behaviour, which highlights the potential opportunity afforded by microwave-assisted defibrillation of BCP for food applications.
Construction of Silk Fibroin 3D Microfiber Scaffolds and Their Applications in Anti-Osteoporosis Drug Prediction
Authors: Xu Hua , Huang Mengfan , Zhou Mengyuan , Guo Rong , Qin Kunming , Dong Zibo
Published: December 2024

Silk microfiber scaffolds have garnered increasing interest due to their outstanding properties, with degumming being the process used to extract the sericin from the cocoon. In the present study, an attempt to tune the biodegradation period of silk through degumming with various sodium borohydride (NaBH4) concentrations and degumming times was studied. We considered the process, the number of baths used, and the salt concentration. Herein, we report a novel method of expanding microfibers from two-dimensional (2D) to three-dimensional (3D) using a modified gas-foaming technique. Porous three-dimensional (3D) silk fibroin (SF) scaffolds were fabricated by the SF fibers, which were extracted by the NaBH4 degumming method and NaBH4 gas-foaming approach. This study showed that higher salt concentrations, reaching 1.5% in a double bath, effectively removed sericin from silk fibroin, resulting in clean, smooth 3D scaffolds. These scaffolds were then fabricated using a freeze-drying method. The scaffolds were then submerged in solutions containing semen cuscutae (SC) and their surfaces were coated with various percentages of total flavonoids. The scaffolds had no toxicity to the cells in vitro. This work provides a new route for achieving a TFSC-loaded scaffold; it is proved that the coated silk fibroin fiber scaffold has excellent compatibility. Compared with non-drug-loaded silk scaffolds, drug-loaded silk scaffolds promote cell growth.
Metal Ion Release from Orthodontic Archwires: A Comparative Study of Biocompatibility and Corrosion Resistance
Authors: Mikulewicz Marcin , Suski Piotr , Tokarczuk Oskar , Warzyńska-Maciejewska Magdalena , Pohl Paweł , Tokarczuk Beata
Published: December 2024

This study investigates the release of metal ions from commonly used orthodontic archwires, specifically, stainless steel (SS), nickel–titanium (NiTi), chromium–cobalt (CrCo), and titanium–molybdenum (TMA) alloys. To simulate oral conditions, each type of wire was immersed in artificial saliva at body temperature for a four-week period. Ion release levels were analyzed through ICP-OES mass spectrometry. The findings indicate that NiTi and CrCo wires released significantly higher amounts of nickel (Ni) and chromium (Cr) ions compared to SS wires. These findings underscore the potential risk of allergic reactions, particularly to nickel, and emphasize the need for careful consideration of biocompatibility in orthodontic material selection. This research also provides valuable insights aimed at minimizing adverse reactions in patients, especially those with metal allergies.
Composition, Anti-Diabetic, and Antioxidant Potential of Raphanus sativus Leaves
Authors: Kajszczak Dominika , Sosnowska Dorota , Frąszczak Barbara , Podsędek Anna
Published: December 2024

Limiting and/or slowing down the starch digestion process and consequently the release of glucose can be an important strategy for the prevention of type 2 diabetes (T2D). The aim of the current in vitro study was to assess the anti-diabetic and antioxidant potential of red radish leaves of the Carmen, Jutrzenka, Saxa, and Warta cultivars. In the context of anti-diabetic activity, the effect of leaves on potato starch digestion and free glucose binding, as well as inhibitory effects of leaf extracts against α-amylase and α-glucosidase and non-enzymatic glycation (AGEs) were determined. The basic chemical composition, quantitative composition of phenolic compounds, and antioxidant activity of leaves were also estimated. This study showed that all radish leaves inhibited the breakdown of potato starch and showed their ability to bind glucose. This activity was correlated with the content of hydroxycinnamic acids, protein and dietary fiber while flavones was probably responsible for glucose binding. Leaf extracts inhibited α-glucosidase activity and formation of AGEs but were practically inactive towards α-amylase. Inhibition of α-glucosidase activity was related to the content of proanthocyanidins and inhibition of AGEs formation to flavonols. These results point to radish leaves, especially the Warta and Jutrzenka cultivars, as a potential natural remedy for treating T2D.
Seedless and Surfactant-Free Synthesis of Polyhedron Gold Nanocrystals Enclosed by High-Index Facets for Enhanced Electrochemical Detection of Benzoyl Peroxide in Flour
Authors: Wang Zixuan , Gao Qianlong , Yao Kai , Ran Wei , Li Ying , Jin Yushen , Shao Bing , Sun Jiefang
Published: December 2024

Polyhedron gold nanocrystals enclosed by high-index facets (HIF-Au NCs) are in high demand but are very difficult to prepare. To address this issue, we presented a simple, seedless method for synthesizing uniform HIF-Au NCs in an aqueous solution, which remarkably reduced the synthesis difficulty. Interestingly, the protonated N2H4 which served as both the reducing and capping agent played a crucial role in modulating the kinetic growth of the HIF-Au NCs. The resulting HIF-Au NCs exhibited distinct electronic oxidation inertness toward alcohol but demonstrated exceptional activity in the electrocatalytic oxidation of peroxides. To demonstrate their sensing capabilities, an electrode decorated with HIF-Au NCs was used to selectively detect benzoyl peroxide (BPO) in flour. BPO is a prohibited whitening agent that may be illegally added to flour and other products, posing potential health risks. The results demonstrate that this assay offers a promising method for the sensitive and selective detection of BPO. In conclusion, this research provides a straightforward pathway for obtaining HIF-Au NCs and further demonstrates their use in electronic sensing. It is expected that HIF-Au NCs will serve as a powerful tool in plasmon-enhanced spectroscopies, catalysis, and sensing applications.
Remediation of Cr(VI) Polluted Groundwater Using Zero-Valent Iron Composites: Preparation, Modification, Mechanisms, and Environmental Implications
Authors: Yang Manyu , Zhang Xueyan , Sun Yongchang
Published: December 2024

The extensive application of chromium (Cr) in many industries has inevitably resulted in the release of Cr(VI) into the groundwater environment, thus posing damage to the ecosystem and human health. Nano zero-valent iron (nZVI) has been widely studied and applied in the remediation of Cr(VI)-contaminated water as an ideal material with high reductive capacity, which enables the transformation of teratogenic and carcinogenic Cr(VI) into less toxic Cr(III). This review comprehensively summarizes the preparation and modification methods of nZVI Cr(VI) removal performance and mechanisms by nZVI and modified nZVI materials. The field applications of nZVI-based materials, such as combining the injection well and the permeable reactive barrier (PRB) to remove Cr(VI) in groundwater, have been reported. Subsequently, the potential toxicity of nZVI-based materials to organisms during environmental application has been highlighted in the current study. Finally, the review outlines potential improvements and explores future directions for the use of nZVI-based materials in groundwater contamination remediation.
Preparation and Piezocatalytic Performance of γ-AlON Particles for Dye-Pollutant Degradation Under Ultrasonic Vibration
Authors: Zhu Dan , Wang Yanyan , Xiao Le , Dai Yu , Wu Jian
Published: December 2024

Piezocatalytic materials have attracted widespread attention in the fields of clean energy and water treatment because of their ability to convert mechanical energy directly into chemical energy. In this study, γ-AlON particles synthesised using carbothermal reduction and nitridation (CRN) were used for the first time as a novel piezocatalytic material to degrade dye solutions under ultrasonic vibration. The γ-AlON particles exhibited good performance as a piezocatalytic material for the degradation of organic pollutants. After 120 min under ultrasonic vibration, 40 mg portions of γ-AlON particles in 50 mL dye solutions (10 mg/L) achieved 78.06%, 67.74%, 74.29% and 64.62% decomposition rates for rhodamine B (RhB), methyl orange (MO), methylene blue (MB) and crystal violet (CV) solutions, respectively; the fitted k values were 13.35 × 10−3, 10.79 × 10−3, 12.09 × 10−3 and 8.00 × 10−3 min−1, respectively. The piezocatalytic mechanism of γ-AlON particles in the selective degradation of MO was further analysed in free-radical scavenging activity experiments. Hydroxyl radicals (•OH), superoxide radicals (•O2−), holes (h+) and electrons (e−) were found to be the main active substances in the degradation process. Therefore, γ-AlON particles are an efficient and promising piezocatalytic material for the treatment of dye pollutants.
Determination of Lactoferrin Using High-Frequency Piezoelectric Quartz Aptamer Biosensor Based on Molecular Bond Rupture
Authors: Wu Haizhi , Si Shihui , Li Zheng , Su Jiayou , Jia Shangguan , He Hao , Peng Chengcheng , Cheng Tongqiang , Wu Qian
Published: December 2024

In this study, an aptamer biosensor for detecting lactoferrin (LF) was developed using piezoelectric quartz-induced bond rupture sensing technology. The thiol-modified aptamer I was immobilized on the gold electrode surface of the quartz crystal microbalance (QCM) through an Au-S bond to specifically bind LF. It was then combined with aptamer–magnetic beads to amplify the mass signal. The peak excitation voltage was 8 V at the resonance frequency for the 60 MHz gold-plated quartz crystal. When the molecular bond cracking process occurred, the aptamer–magnetic beads combined on the surface of the piezoelectric quartz were removed, which resulted in an increase in quartz crystal resonance frequency. Therefore, the specific detection of LF can be realized. Under optimized experimental conditions, the linear range for LF was 10–500 ng/mL, the detection limit (3σ) was 8.2 ng/mL, and the sample recoveries for actual milk powder samples ranged from 97.2% to 106.0%. Compared with conventional QCM sensing technology, the signal acquisition process of this sensing method is simple, fast, and easy to operate.
Volatile Substances, Quality and Non-Targeted Metabolomics Analysis of Commercially Available Selenium-Enriched Rice
Authors: Zhang Yu , Lian Qianqian , Zhao Jianji , He Yanping , Dai Huang , Liu Xiuying , Zhang Wei , Bi Jie
Published: December 2024

Selenium is an essential trace element for the human body. However, its intake is usually low. Therefore, the production and utilization of selenium-enriched food are currently a research hotspot. Despite the remarkable scientific interest in this topic, only a few of the numerous studies focus on commercially available products. This study examined the nutritional quality, physical and chemical properties, cooking characteristics, and eating quality of four commercially available hot-selling rice types, both selenium-enriched and non-selenium-enriched, and discovered that selenium-enriched rice outperforms ordinary rice in terms of both nutritional quality and taste. In addition, we employed the gas chromatography–ion mobility spectrometry (GC-IMS) technique to evaluate the volatile chemicals of rice. Some of the chemicals that made selenium-rich rice taste different from regular rice were pentanal, (E)-2-Hexen-1-ol, ethyl-3-methyl butanoate, 2-furan methanol acetate, ethyl heptanoate, ethyl hexanoate, methyl hexanoate, isopentyl pentanoate, and ethyl butyrate. We looked into the metabolite profiles of rice using LC-MS-based untargeted metabolomics to obtain a better idea of the different metabolites that are found in selenium-enriched rice compared to regular rice. We identified a total of 522 metabolites and screened 182, 227, and 100 differential metabolites in selenium-enriched (A) vs. non-selenium-enriched rice (B/C/D) groups, respectively. This study revealed that selenium primarily influenced the metabolism of D-amino acids, starch, sucrose, and linoleic acid in rice. This study systematically analyzed the quality differences between selenium-enriched and non-selenium-enriched rice available on the market. For consumers, it is essential to understand the quality of selenium-rich rice on the market to guide the purchase of rice.
Chelating Properties of N6O-Donors Toward Cu(II) Ions: Speciation in Aqueous Environments and Catalytic Activity of the Dinuclear Complexes
Authors: Cendron Andrea , Chianese Martina , Zarzycki Kamil , Ruzza Paolo , Honisch Claudia , Brasuń Justyna , Carraro Mauro
Published: December 2024

This study focuses on the use of three isostructural N6O donor ligands, specifically known to form complexes with copper ions, to chelate Cu(II) from aqueous solutions. The corresponding Cu(II) complexes feature a dinuclear copper core mimicking the active site of natural superoxide dismutase (SOD) enzymes while also creating a coordination environment favorable for catalase (CAT) activity, being thus appealing as catalytic antioxidant systems. Given the critical role of copper dysregulation in the pathophysiology of Alzheimer’s disease (AD), these complexes may help mitigate the harmful effects of free Cu(II) ions: the goal is to transform copper’s reactive oxygen species (ROS)-generating properties into beneficial ROS-scavenging action. This study investigates the speciation, chelating efficiency, and metal selectivity of these ligands, as well as the antioxidant activity of the resulting complexes under aqueous and physiologically relevant conditions. Additionally, the ligands, equipped with functional groups for attaching targeting moieties, are conjugated with a small peptide that may act as an anti-aggregating agent of β-amyloid peptides, aiming to develop a multifunctional therapeutic strategy against Alzheimer’s disease.
One-Pot Synthesis of Hydroxylated Alkaloids from Sugars via a Pictet–Spengler-Type Reaction
Authors: Zhou Likai , Ma Na , Wu Jilai , Yang Weilin , Feng Lijing , Xie Song , Wang Lili , Chen Hua
Published: December 2024

An efficient and convenient strategy has been successfully developed for the preparation of novel hydroxylated alkaloid derivatives (also called fused multicyclic iminosugars) from p-toluenesulfonylated sugars through a Pictet–Spengler-type mechanism. This method is highly stereoselective, does not require metal catalysts, and capable of conducting gram level reactions (with a 53% yield). Some of such iminosugars had an intermediate antiproliferative effect on HCT116 tumor cells.
Synthesis of Antimicrobial Norlabdane Compounds with Rearranged Cycle B and Molecular Docking Studies
Authors: Ciocarlan Alexandru , Lungu Lidia , Shova Sergiu , Vornicu Nicoleta , Bolocan Natalia , Kulcitki Veaceslav , Aricu Aculina
Published: December 2024

The synthesis of tetra- and pentanorlabdane compounds with rearranged cycle B based on commercially available (+)-sclareolide is reported. Desired compounds were prepared from intermediate ketones via Baeyer–Villiger oxidation. The structures of synthesized compounds were confirmed by spectral IR, 1D (1H, 13C, and DEPT), and 2D (H-COSY, H,C-HSQC, H,C-HMBC, H,N-HMBC, NOESY) NMR analyses, mass-spectrometry and single crystal X-rays diffraction. Two out of the four synthesized compounds showed high antifungal and antibacterial activities comparable to and exceeding standard antifungal (caspofungin) and antibacterial (kanamycin) agents. DFT calculations show that in gas and DCM, compound 4 is more stable than 3 with a difference in the Gibbs free energy of 23.3 kJ/mol and 20.7 kJ/mol, respectively. In water and methanol, compound 3 is slightly more stable, by 2.4 kJ/mol and 2.78 kJ/mol, respectively. Molecular docking to four targets DNA gyrase from E. coli (1KZN), Fabz from P. aeruginosa (1U1Z), dihydrofolate reductase from C. albicans (3QLS) and MurB from E. coli (2Q85) showed good agreement with the results of in vitro evaluation and confirmed the biological activity of compounds 3 and 4, with binding affinities comparable and for some targets exceeding that of Caspofungin and Kanamycin.
Experimental Investigation of the Optical Nonlinearity of Laser-Ablated Titanium Dioxide Nanoparticles Using Femtosecond Laser Light Pulses
Authors: Abdel Samad Fatma , Ali Jasim Mohammed , Mahmoud Alaa , Abd El-Salam Yasmin , Qayyum Hamza , Apsari Retna , Mohamed Tarek
Published: December 2024

In this report, the nonlinear optical (NLO) properties of titanium dioxide nanoparticles (TiO2 NPs) have been explored experimentally using femtosecond laser light along with the Z-scan approach. The synthesis of TiO2 NPs was carried out in distilled water through nanosecond second harmonic Nd:YAG laser ablation. Characterization of the TiO2 NPs colloids was conducted using UV-visible absorption spectroscopy, transmission electron microscopy (TEM), inductively coupled plasma (ICP), and energy-dispersive X-ray spectroscopy (EDX). The TEM analysis indicated that the size distribution and average particle size of the TiO2 NPs varied from 8.3 nm to 19.1 nm, depending on the laser ablation duration. The third-order NLO properties of the synthesized TiO2 NPs were examined at different excitation laser wavelengths and incident powers through both open- and closed-aperture Z-scan techniques, utilizing a laser pulse duration of 100 fs and a high repetition rate of 80 MHz. The nonlinear absorption (NLA) coefficient and nonlinear refractive (NLR) index of the TiO2 NPs colloidal solutions were found to be influenced by the incident power, excitation wavelength, average size, and concentration of TiO2 NPs. Maximum values of 4.93 × 10⁻⁹ cm/W for the NLA coefficient and 15.39 × 10⁻15 cm2/W for the NLR index were observed at an excitation wavelength of 800 nm, an incident power of 0.6 W, and an ablation time of 15 min. The optical limiting (OL) effects of the TiO2 NPs solution at different ablation times were investigated and revealed to be concentration and average size dependent. An increase in concentration results in a more limiting effect.
Acceptance, Needs, and Demands for Nutritional mHealth Support in Patients with Cardiovascular Disease
Authors: Mohajeri Darya , Jahre Lisa Maria , Bäuerle Alexander , Schieffers Theresa , Messiha Daniel , Rammos Christos , Teufel Martin , Rassaf Tienush , Lortz Julia
Published: December 2024

Background: Cardiovascular diseases (CVDs) are the leading causes of death globally. Managing risk factors and preventing atherosclerosis and its progress, especially with lifestyle changes, are highly important. Smartphone-based mobile health (mHealth) strategies allow easily accessible assistance for healthy nutrition. This study aimed to assess the acceptance and outline the needs and demands for a nutritional mHealth tool by analyzing the desired characteristics. Methods: A cross-sectional study was conducted between August 2022 and September 2023 targeting 398 individuals with atherosclerosis. Acceptance, needs, and demands regarding mHealth, sociodemographic, medical, psychometric, and electronic health (eHealth) data were assessed. Multiple hierarchical regression analyses were conducted to determine the predictors of acceptance. Results: High acceptance for nutritional mHealth was reported by 88.4% (n = 274). Significant predictors of acceptance were age (β = −0.01, p = 0.002), diabetes (β = 0.20, p = 0.041), depressive symptoms (β = −0.02, p = 0.017), digital confidence (β = 0.17, p = 0.001), Internet anxiety (β = −0.18, p = 0.004), and the Unified Theory of Acceptance and Use of Technology (UTAUT) predictors effort expectancy (β = 0.23, p < 0.001) and social influence (β = 0.53, p < 0.001). Preferences included handheld devices, permanent use (86.5%), and weekly (44.5%) new content of 10 to 30 min (79%). Conclusions: These results summarize the patients’ preferences for individualized mHealth tools to ensure their effectiveness. Especially regarding the secondary prevention of CVDs, mHealth can be a helpful resource. The high acceptance rate and specific preferences outlined in this study form a strong basis for the development of mHealth tools with a focus on nutritional support in patients with CVDs.
Assessing the Benefit of Dietary Choline Supplementation Throughout Adulthood in the Ts65Dn Mouse Model of Down Syndrome
Authors: Tallino Savannah , Etebari Rachel , McDonough Ian , Leon Hector , Sepulveda Isabella , Winslow Wendy , Bartholomew Samantha K. , Perez Sylvia E. , Mufson Elliott J. , Velazquez Ramon
Published: December 2024

Background/Objectives: Down syndrome (DS) is the most common cause of early-onset Alzheimer’s disease (AD). Dietary choline has been proposed as a modifiable factor to improve the cognitive and pathological outcomes of AD and DS, especially as many do not reach adequate daily intake levels of choline. While lower circulating choline levels correlate with worse pathological measures in AD patients, choline status and intake in DS is widely understudied. Perinatal choline supplementation (Ch+) in the Ts65Dn mouse model of DS protects offspring against AD-relevant pathology and improves cognition. Further, dietary Ch+ in adult AD models also ameliorates pathology and improves cognition. However, dietary Ch+ in adult Ts65Dn mice has not yet been explored; thus, this study aimed to supply Ch+ throughout adulthood to determine the effects on cognition and DS co-morbidities. Methods: We fed trisomic Ts65Dn mice and disomic littermate controls either a choline normal (ChN; 1.1 g/kg) or a Ch+ (5 g/kg) diet from 4.5 to 14 months of age. Results: We found that Ch+ in adulthood failed to improve genotype-specific deficits in spatial learning. However, in both genotypes of female mice, Ch+ significantly improved cognitive flexibility in a reverse place preference task in the IntelliCage behavioral phenotyping system. Further, Ch+ significantly reduced weight gain and peripheral inflammation in female mice of both genotypes, and significantly improved glucose metabolism in male mice of both genotypes. Conclusions: Our findings suggest that adulthood choline supplementation benefits behavioral and biological factors important for general well-being in DS and related to AD risk.
Antioxidant and Antiaging Activity of Houttuynia cordata Thunb. Ethyl Acetate Fraction in Caenorhabditis elegans
Authors: Kim Hyeon-Ji , Mun Ji-Su , Oh Suk-Heung , Kim Jun-Hyung
Published: December 2024

Background/Objectives: In aerobic organisms, such as humans, oxygen radicals are inevitably produced. To counteract oxidation, the body generates antioxidant substances that suppress free radicals. However, levels of reactive oxygen species (ROS) increase due to aging and lifestyle factors, leading to exposure to various diseases. While synthetic antioxidants offer advantages like high stability, low cost, and availability, their safety remains controversial. This study aimed to investigate the antioxidant and antiaging activities of Houttuynia cordata (HC), which is rich in flavonoids and has excellent antioxidant properties, using Caenorhabditis elegans as a model. Methods: Extraction and fractionation of HC were performed to evaluate antioxidant activities (DPPH, ABTS, superoxide radical scavenging activity) and antiaging effects (lifespan). The ethyl acetate fraction (EAF) with the highest activity was selected for further investigation. Results: The EAF of HC exhibited high levels of polyphenols and flavonoids, presenting the highest DPPH, ABTS, and superoxide radical scavenging activities. This fraction increased the activity of antioxidant enzymes in nematodes in a concentration-dependent manner and provided resistance to oxidative stress, reducing ROS accumulation. Additionally, the fraction enhanced the lifespan of nematodes, improved resistance to heat stress, increased survival rates, and decreased the accumulation of aging pigments (lipofuscin). The expression of daf-2, daf-16, and sir-2.1, proteins directly involved in nematode aging, was confirmed. Liquid chromatography/tandem mass spectrometry identified quercitrin in the HC extract, which may contribute to its antioxidant and antiaging effects. Conclusions: The EAF of HC demonstrates significant potential for influencing antioxidant and antiaging, as evidenced by functional investigations using C. elegans.
Effect of Elderberry (Sambucus nigra L.) Extract Intake on Normalizing Testosterone Concentration in Testosterone Deficiency Syndrome Rat Model Through Regulation of 17β-HSD, 5α-Reductase, and CYP19A1 Expression
Authors: Kim Jiyeon , An Jinho , Song Youngcheon , Jang Mincheol , Kong Hyunseok , Kim Sangbum
Published: December 2024

Background/Objectives. Men experience Leydig cell and mitochondrial dysfunction due to the accumulation of reactive oxygen species during aging, leading to hormonal imbalances in the body. This results in symptoms of testosterone deficiency syndrome (TDS) as testosterone levels decline. Consequently, there is a growing need for alternative therapies, such as phytotherapy, to regulate testosterone secretion. Methods. In this study, we evaluated the potential of elderberry extract powder (KSB191) as a functional ingredient for improving TDS by analyzing its mechanism in regulating testosterone imbalance. The major compounds of KSB191 were rutin and fructose–leucine, and the efficacy of KSB191 was confirmed by observing increases in total testosterone, free testosterone, and sperm motility in an aged rat model with decreased testosterone levels. Additionally, we assessed safety by analyzing levels of prostate-specific antigen, alanine aminotransferase, aspartate aminotransferase, and creatinine. Results. To confirm the effectiveness of KSB191 in increasing testosterone synthesis and inhibiting its breakdown, we analyzed the expression levels of genes related to testosterone synthesis and degradation in the testis tissue. KSB191 not only increases the expression levels of enzymes (3β-HSD, CYP17A1, and 17β-HSD) that catalyze testosterone synthesis in Leydig cells, but also reduces the expression of enzymes (5α-reductase and CYP19A1) that degrade testosterone, thereby enhancing testosterone production in the body. Conclusions. KSB191 is predicted to be a novel functional ingredient that acts on Leydig cells and increases testosterone synthesis (particularly, the increase in free testosterone), ultimately alleviating the symptoms of TDS.
4-Hydroxynonenal from Mitochondrial and Dietary Sources Causes Lysosomal Cell Death for Lifestyle-Related Diseases
Authors: Yamashima Tetsumori
Published: December 2024

Excessive consumption of vegetable oils such as soybean and canolla oils containing ω-6 polyunsaturated fatty acids is considered one of the most important epidemiological factors leading to the progression of lifestyle-related diseases. However, the underlying mechanism of vegetable-oil-induced organ damage is incompletely elucidated. Since proopiomelanocortin (POMC) neurons in the hypothalamus are related to the control of appetite and energy expenditure, their cell degeneration/death is crucial for the occurrence of obesity. In patients with metabolic syndrome, saturated fatty acids, especially palmitate, are used as an energy source. Since abundant reactive oxygen species are produced during β-oxidation of the palmitate in mitochondria, an increased amount of 4-hydroxy-2-nonenal (4-HNE) is endogenously generated from linoleic acids constituting cardiolipin of the inner membranes. Further, due to the daily intake of deep-fried foods and/or high-fat diets cooked using vegetable oils, exogenous 4-HNE being generated via lipid peroxidation during heating is incorporated into the blood. By binding with atheromatous and/or senile plaques, 4-HNE inactivates proteins via forming hybrid covalent chemical addition compounds and causes cellular dysfunction and tissue damage by the specific oxidation carbonylation. 4-HNE overstimulates G-protein-coupled receptors to induce abnormal Ca2+ mobilization and µ-calpain activation. This endogenous and exogenous 4-HNE synergically causes POMC neuronal degeneration/death and obesity. Then, the resultant metabolic disorder facilitates degeneration/death of hippocampal neurons, pancreatic β-cells, and hepatocytes. Hsp70.1 is a molecular chaperone which is crucial for both protein quality control and the stabilization of lysosomal limiting membranes. Focusing on the monkey hippocampus after ischemia, previously we formulated the ‘calpain–cathepsin hypothesis’, i.e., that calpain-mediated cleavage of carbonylated Hsp70.1 is a trigger of programmed neuronal death. This review aims to report that in diverse organs, lysosomal cell degeneration/death occurs via the calpain–cathepsin cascade after the consecutive injections of synthetic 4-HNE in monkeys. Presumably, 4-HNE is a root substance of lysosomal cell death for lifestyle-related diseases.
City Residents Play a Pivotal Role in Managing Global Food Security While Improving Human Health and Minimizing Environmental Footprints
Authors: Drangert Jan-Olof
Published: December 2024

Background/Objectives: Improved global data allow for a new understanding of what impact the food we produce, eat and dispose of has on the environment, human health and Nature’s resources. The overall goal is to guide decision-makers and individuals by providing in-depth knowledge about the effects of their dietary preferences on human and environmental health. Methods: The method is to investigate ways to reduce environmental degradation and to secure healthy food supplies in an urbanizing world, and to quantify the options. Results: Reviewed articles show that by eating less meat-based food and more plant-based and soilless food, as well as reducing food waste and recycling urban-disposed nutrients as fertilizers, we could reduce agriculture’s land requirement by 50% to 70% while still securing a healthy food supply. Less land under cultivation and pasture would reduce global emissions to air and water to a similar extent, and allow Nature to reclaim freed areas in order to catch more carbon and rejuvenate biodiversity. Thus, we could avoid further environmental degradation such as the current clearing of new fields needed under a business-as-usual regime. Presently, some 17 million people die each year due to poor diets, which is more than double the 7 million deaths since the onset of the COVID-19 pandemic. A return to more plant-based diets with unchanged intake of proteins but less calories, sugar, salt and fat combined with less red meat and ultra-processed food would reduce foremost non-communicable diseases by up to 20% and prolong life. The article suggests that the international focus has gradually turned to the food sector’s big contribution to climate change, biodiversity loss and harmful chemicals as well as to poor human health. It argues that this century’s rapid population growth and urbanization give urban residents a pivotal role in food’s impact on agricultural areas, which today cover half of the globe’s inhabitable areas. Their food demand, rather than the activities of farmers, fishermen or loggers, will guide remedial measures to be taken by individuals, industry and the public sector. A tool to calculate the potential environmental footprints of individual or societal measures is presented. Conclusions: Measures to make the agrifood sector more sustainable are still pending full recognition in international fora such as the UN COP Summits. Smart cities fitted with infrastructures to recycle macro- and micro-nutrients and organic matter have the potential to ameliorate human-induced impacts such as emissions to air and water bodies, crossing planetary boundaries, and polluting extraction of N (nitrogen), P (phosphorus) and K (potassium). Rapid results are within reach since dietary change and the turn-around time of nutrients in food is short compared to decades or centuries for recycled materials in cars or buildings.
Beyond Nutritional Treatment: Effects of Fitwalking on Physical Capacity and Intestinal Barrier Integrity in BMI-Stratified IBS Patients
Authors: Bianco Antonella , Russo Francesco , Prospero Laura , Riezzo Giuseppe , Franco Isabella , D’Attoma Benedetta , Ignazzi Antonia , Verrelli Nicola , Bagnato Claudia Beatrice , Goscilo Francesco , Mallardi Domenica , Linsalata Michele , Bonfiglio Caterina , Pesole Pasqua Letizia , Ferro Annalisa
Published: December 2024

Background: Irritable bowel syndrome (IBS) and obesity are associated with intestinal barrier alterations that result in low-grade inflammation. Zonulin and intestinal fatty acid-binding protein (I-FABP) assess gut barrier health, while urinary indican concentrations reflect dysbiosis in the small intestine. Physical activity, such as Fitwalking, aids weight management and improves intestinal permeability. This study assesses the impact of a 12-week Fitwalking program on intestinal barrier health in IBS patients categorized by body mass index (BMI). Methods: Fifty-seven mild IBS patients were categorized as obese (OB = 18), overweight (OW = 24), or normal weight (NW = 15) and assigned to a walking group. Participants walked thrice weekly at moderate intensity for 60 min per session, using the specific Fitwalking technique, supervised by staff. Results: No significant changes in biochemical or anthropometric variables were observed. However, Fitwalking improved the Global Physical Capacity Score (GPCS) by 46%, 48%, and 24% in the NW, OW, and OB groups. Post-intervention, serum zonulin levels notably decreased in OB individuals, suggesting reduced inflammation. OW patients unexpectedly showed increased fecal zonulin levels. OB participants experienced decreased urinary indican levels. Zonulin levels positively correlated with BMI and inversely with GPCS. Conclusions: Regular exercise benefits the intestinal barrier, especially in obese IBS patients. Monitoring zonulin and I-FABP may offer insights into gut barrier integrity and GI injury severity. Future studies should explore longer intervention durations, larger populations, and advanced diagnostic tools to validate findings and investigate the mechanisms behind exercise-induced changes in intestinal permeability and gut health markers.
Nutrient Supplements for Young Children and Mothers’ Self Medication with Over-the-Counter Drugs During the COVID-19 Pandemic
Authors: Aydın Aksoy Esin , Güçiz Doğan Bahar , Yalçın Sıddıka Songül
Published: December 2024

Background: The restriction of access to health services during the COVID-19 pandemic has led to an increase in self-medication. This study aims to examine mothers’ use of nutrient supplements with over-the-counter (OTC) medications for their children, including instances of self-medication for themselves. The study also explores maternal characteristics associated with this behavior, the specific medications used, and the reasons for use. Method: In this descriptive study, 450 mothers with children aged 2 to 6 years in Türkiye were recruited through social media platforms. Questions focused on whether mothers used supplements for themselves and their children, types of products, frequency, and reasons for use. Multivariable binary logistic regression was conducted to examine the factors associated with OTC medication use for children. Result: Nearly half of the mothers reported administering OTC medications to their children. Factors associated with this practice included the child’s age (specifically 48–72 months), attendance at nursery, perceived underweight status, and regular health visits with a pediatrician. Additionally, mothers who frequently used medications without a doctor’s recommendation were 5.8 times more likely to give OTC drugs to their children. Maternal self-medication was significantly associated with an increased likelihood of OTC medication use for children (OR = 12.1). The most commonly used supplements included vitamin D, fish oil, multivitamins, vitamin C, immune boosters, zinc, probiotics, herbal teas, oral/nasal sprays, throat lozenges, and aspirin, with the primary purposes being prevention and treatment. Conclusions: The administration of OTC medications in young children, who rely heavily on maternal care should be more closely monitored to ensure their safety and well-being, especially during epidemics.
Variable Assembly and Procapsid Binding of Bacteriophage P22 Terminase Subunits in Solution
Authors: Cabral Julia Elise , Qiu Yanfei , Heck Albert J. R. , McNulty Reginald
Published: December 2024

Concatemeric viral DNA is packaged into bacteriophage P22 procapsids via a headful packaging mechanism mediated by a molecular machine consisting of small (gp3) and large (gp2) terminase subunits. Although a negative stain reconstruction exists for the terminase holoenzyme, it is not clear how this complex binds the dodecameric portal protein located at a 5-fold mismatch vertex. Herein, we describe new assemblies for the holoenzyme. Both native mass spectrometry and transmission electron microscopy reveal that the P22 terminase complex adopts three main assemblies, which include a nonameric S-terminase bound to two L-terminase 1(gp3)9:2(gp2), two nonameric S-terminase bound to five L-terminase 2(gp3)9:5(gp2), and three nonameric S-terminase bound to seven L-terminase 3(gp3)9:7(gp2). Native agarose gel electrophoresis shows that the terminase complex interacts with procapsids with mild crosslinking. These results herein illustrate the P22 terminase complex can adopt a variety of conformations and assembly states.
Combinatorial Treatment with Praziquantel and Curcumin Reduces Clonorchis sinensis Parasite Burden and Clonorchiasis-Associated Pathologies in Rats
Authors: Lee Soon-Ok , Chu Ki Back , Yoon Keon-Woong , Heo Su In , Song Jin-Ho , Li Jianhua , Hong Sung-Jong , Quan Fu-Shi
Published: December 2024

Background/Objectives: Clonorchiasis is a foodborne parasitic disease that can lead to severe biliary fibrosis and cholangiocarcinoma. While praziquantel (PZQ) is available for clonorchiasis treatment, it cannot revert the histopathological damage incurred through parasite-induced fibrosis. Curcumin (CUR) is an emerging experimental drug possessing anti-inflammatory and fibrosis-alleviating effects, thus signifying its potential as an anthelmintic drug. Here, we evaluated the effect of CUR+PZQ combinatorial drug treatment on C. sinensis infection as well as its effect on ameliorating fibrotic tissue damage in rats. Methods: Worm viabilities following CUR and PZQ treatments were confirmed through microscopy and tetrazolium salt absorption. Anthelminthic effect and hepatobiliary damage mitigation in rats were determined by quantifying worm recovery, histopathological staining, and enzyme-linked immunosorbent assay. Results: CUR+PZQ at LD50 doses demonstrated a time- and dose-dependent antiparasitic effect in vitro, which was markedly greater than either drug alone. Rats were infected with C. sinensis, and drugs were administered at 1 and 4 weeks post-infection (wpi) to assess drug-induced changes in worm burden. Significant reductions in worm burden recoveries were observed following CUR+PZQ treatment at both time points, accompanied by markedly reduced serum and mucosal IgG responses. ALT and AST levels were also substantially lower in combinatorial drug treatment groups than controls. Histopathological examinations confirmed that parasite-induced bile duct lumen widening and liver fibrosis were suppressed at 1 wpi, implying that CUR+PZQ co-treatment can alleviate clonorchiasis-associated pathologies. Conclusions: Our findings indicate that CUR+PZQ co-treatment improved parasite clearance and promoted the resolution of hepatobiliary tissue damage resulting from chronic clonorchiasis.
Genetic Diversity Analysis and Polyploid Induction Identification of Idesia polycarpa
Authors: Luo Xiaomei , Liu Yunke , Lei Yuting , He Zhoujian , Gong Xiao , Ye Meng , Xiao Qiangang
Published: December 2024

Idesia polycarpa from Sichuan is a valuable germplasm with high economic potential, but it faces variety scarcity. To address this, this study collected 16 varieties (lines), identifying IpHT1 as a promising parent due to its high oil content (38.5%) and red fruits. Polyploid induction via adding 0.50% colchicine to Murashige and Skoog (MS) medium yielded 520 IpHT1 mutagenized seedlings. Subsequently, flow cytometry (FCM) was performed on 401 morphologically variant seedlings which had been initially screened, resulting in the identification of 15 suspected triploids, 35 suspected tetraploids, and 3 chimeras. Furthermore, fluorescence in situ hybridization (FISH) analysis found that the probe (AG3T3)3 had terminal signals at both ends of each chromosome, allowing for the counting of 42 chromosomes in diploids and 84 in tetraploids. The probe 5S rDNA showed 2, 3, and 4 hybridization signals in the interphase nuclei of diploid, triploid, and tetraploid cells, respectively, but the probe (GAA)6 failed to produce any signal on I. polycarpa chromosomes. Ultimately, 18 polyploids were selected, including 7 triploids and 11 tetraploids. Triploids and tetraploids showed significant leaf morphological and physiological differences from diploids. Consequently, this study successfully established a polyploid breeding system for I. polycarpa, thereby enhancing its genetic diversity and breeding potential.
Sustainable Processes Reusing Potassium-Rich Biomass Ash as a Green Catalyst for Biodiesel Production: A Mini-Review
Authors: Tsai Chi-Hung , Tsai Wen-Tien
Published: December 2024

To mitigate the emissions of greenhouse gases (GHGs) from fossil fuels, the use of biodiesel and its sustainable production have been receiving more attention over the past decade, especially for the reuse of waste cooking oils and non-edible oils as starting feedstocks. For the biodiesel production process, the suitability of a green catalyst is a core function in the transesterification reaction. Heterogeneous (solid-state) catalysts are generally superior to homogeneous (liquid-state) catalysts due to several significant advantages such as no saponification products formed, recyclability, and less equipment corrosion. Recent studies also revealed that heterogeneous solid base catalysts were widely used for the production of biodiesel. Furthermore, the use of biomass-based ash derived from herbaceous and agricultural biomass is increasing rapidly because of its environmental sustainability, high biodiesel yield, and low catalyst cost. To highlight alternative catalysts from biomass residues, this mini-review paper thus focused on a summary of various heterogeneous potassium-rich ash materials, which were used as green catalysts for the sustainable production of biodiesel. Due to the abundant quantity and chemical compositions, it was found that ash derived from cocoa pod husk may be the most commonly used solid base catalyst for producing biodiesel in the literature. Finally, future perspectives on biodiesel production by adopting emerging technologies and using high-potassium (K) biomass ash as a green catalyst were also addressed.
Effect of Froth on the Interaction Between Coal Particles and Cake Structures in the Dewatering Process of Clean Coal
Authors: Chen Ruxia , Dong Xianshu , Feng Zeyu , Fan Yuping , Ma Xiaomin
Published: December 2024

Effective coal slurry water solid–liquid separation is indispensable for the recycling and sustainable development of coal resources. The interaction between bubble and coal particles plays a critical role in the process of dewatering for clean coal. In this study, we firstly conducted a comprehensive investigation of the impact of froth on the interactions between coal particles by rheological measurement and particle aggregation behavior. Furthermore, the macroscopic dewatering performance of coal slurry in the presence of froth and its microscopic cake structure were investigated using the filtration test and X-ray microtomography (CT). It was found that the interaction between coal particles in the presence of froth was enhanced as a result of the dynamic shear value, combined with the large floc size and compact structure, which led to a higher cake moisture and higher filtration velocity. The CT results indicated that the enhanced interaction of particles in the presence of froth also led to a dense microstructure of the filter cake. The porosity of the filter cake decreased to 2.05% when the aeration time increased from 0 s to 90 s, the throat radius in the filter cake was reduced to 1.32 μm, and the number of throat passages was reduced to one third. Multiple blind pores and low coordination numbers led to a poor connectivity of the pore network and high moisture content.