bluuebunny/crossref_metadata_2025 huggingface dataset:
Optical water characterization and atmospheric correction assessment of estuarine and coastal waters around the AERONET-OC Bahia Blanca
Authors: Arena Maximiliano , Pratolongo Paula , Loisel Hubert , Tran Manh Duy , Jorge Daniel Schaffer Ferreira , Delgado Ana Laura
Published: February 2024

The site AERONET-OC Bahía Blanca (BB-AERONET-OC) is located at the mouth of the Bahía Blanca Estuary, Argentina (Southwestern Atlantic Ocean), a coastal system defined by its high suspended loads and relatively low colored dissolved organic matter. The typically high turbidity of these waters makes the BB-AERONET-OC distinctive within the AERONET-OC network stations, providing exceptional opportunities not only for the validation of atmospheric correction algorithms but also for the development of regional algorithms for coastal complex waters. A SeaWiFS Photometer Revision for Incident Surface Measurements (SeaPRISM) instrument was deployed in January 2020 in the upper rail of a Mareograph Tower, a 15 m tall structure, located 10 miles away from the coast in optically deep waters. In this work we used the remote sensing reflectance (Rrs) derived from the BB-AERONET-OC measurements along with in situ hyperspectral radiometric data to classify optical water types (OWTs). We assigned each Rrs(λ) spectra to one of the five OWTs defined by Tran et al., and OWTs were further characterized with the concentrations of optically significant components (chlorophyll-a and suspended particulate matter) and inherent optical properties (absorptions of phytoplankton, non-algal particles, and dissolved organic matter), retrieved from water samples obtained simultaneously with radiometric spectra. Based on a match-up exercise with in situ data, different schemes of atmospheric correction methods were applied to Sentinel-3 Ocean and Land Colour Instrument (OLCI) images. The operational product OLCI Level 2 European Space Agency (ESA) standard (hereafter referred to as “Standard Neural Network (NN)”) proves to be the most suitable atmospheric correction algorithm, which was then used to describe spatial patterns and temporal variability of the different OWTs in the region. The BB-AERONET-OC site is located in a sharp transition between estuarine and coastal waters that present contrasting optical conditions: OWT 4 dominates over time (73.72% of the observations), followed by OWT 3 (24.74%) and OWT 5 (1.53%). OWTs 4 and 5 are associated with turbid waters of the Bahía Blanca Estuary, especially OWT 5, which typifies the very turbid waters from the inner estuary, with the particulate load dominated by mineral sediments and detritus. OWT 3, in turn, depicts the eutrophic coastal waters of the inner shelf. The variability of OWTs and the relative contribution of organic and inorganic compounds to the suspended material would be mostly related with the prevalence of northwest winds in the area, which would drive the export of estuarine sediments to the shelf.
Simulating micelle self-assembly to assess potential for viscosity build in surfactant formulations
Authors: Lavagnini Ennio , Booth Jonathan , Helm Katy , El-Benni Ferdaous , Warren Patrick B. , Bray David J. , Anderson Richard L.
Published: February 2024

Self-assembly of surfactants into complex structures is key to the performance of many formulated products, which form a significant fraction of the world’s manufactured goods. Here we adopt the dissipative particle dynamics simulation approach to explore the self-assembly process of surfactants, with the aim of understanding what information can be obtained that may correlate with an increased zero-shear viscosity of surfactant based products. To this end we experimentally measured the zero-shear viscosity of mixed micelle systems comprised of cocoamidopropyl betaine (CAPB) and sodium lauryl sarcosinate (SLSar), as a function of the CAPB/SLSar mass ratio and pH, and characterised the early stages of self-assembly of the same systems computationally. From simulation we identify three distinct behaviors in the micellar self-assembly process (logarithmic, linear and cubic growth) which we find show some degree of correlation with the experimental zero-shear viscosity. Owing to the relatively short simulation times required, this may provide formulation scientists with a practical route to identify regions of interest (i. e. those with a desired zero-shear viscosity) prior to synthesising de novo (potentially natural) surfactants.
Gender expression and gender identity in virtual reality: avatars, role-adoption, and social interaction in VRChat
Authors: Zhang Jingyi , Juvrud Joshua
Published: February 2024

Introduction: This study examines the complex relationship between gender, virtual reality, and social interaction.

Methods: Utilizing unobtrusive observations and interviews within the VRChat platform, this research explored avatar choices, interactions, and full-body tracking (FBT) technology utilization as they related to users’ expressions and perceptions of gender in virtual reality (VR).

Results: The findings revealed that cultural background plays a significant role in shaping gender expression and perception. Results demonstrated the fluidity of gender expression in virtual environments, highlighting how users can challenge and subvert traditional gender norms, and the potential of virtual reality as a tool for experiential learning, fostering cross-cultural understanding, and promoting inclusive and diverse gender expressions.

Discussion: This study contributes to the emerging body of literature on virtual reality and gender, providing insights that can inform future research and technology development.
An investigation of the perceptions of laboratory animal welfare issues among undergraduate and graduate veterinary students in southeastern China
Authors: Yan Shihong , Li Hongyang , Lin Jin , Chen Huimin , Liu Shasha , Diao Hongxiu
Published: February 2024

Animal experiments have played a significant role in advancing scientific knowledge and enhancing people's quality of life. In order to better understand the opinions and knowledge of veterinary students in the domain of laboratory animal welfare and to explore and advance the teaching methods used in animal ethics education, a questionnaire was designed and used to conduct a survey among undergraduate and postgraduate students majoring in veterinary medicine. The survey encompassed various topics, such as students' level of knowledge about animal welfare, their perspectives on laboratory animals, their proficiency with animal experiments, and their opinions on teaching methods and content. The respondents were a total of 150 undergraduate students and 148 graduate students. The survey results indicated that most students expressed a strong sense of responsibility for the safeguarding of the welfare of experimental animals. However, there were a few students who lacked compassion for animals. Additionally, there was a general lack of basic theoretical knowledge of animal ethics and an inadequate grasp of experimental techniques among current students. Furthermore, most of the participants expressed a strong sense of responsibility to advocate for animal welfare. Although a substantial number of students were unaware of the existence of agencies for the supervision of work involving laboratory animals, they supported teaching and supervision in the domain of animal welfare and were open to various teaching methods and topics of content. In conclusion, targeted training and education regarding laboratory animal welfare and ethics should be conducted in the future to address the specific needs of students. This study provides a foundation for future animal welfare education and will help to improve the professional skills and humanistic qualities of veterinary students.
Metabolomics-based study of the effect of dietary N-carbamoylglutamic acid addition to heifers in late pregnancy on newborn calves
Authors: Wang Jiandong , Yu Youli , Guo Yanan , Guo Yansheng , Liang Xiaojun
Published: February 2024

It has been demonstrated that supplementing late-gestation cow diets with NCG (N-carbamoylglutamic acid) increases the serum protein level, boosts immunological function, and increases the birth weight of the calves. However, the underlying mechanism remains unclear. In this experiment, 30 late-gestation Angus heifers almost at same conditions were chosen for this experiment. They were randomly divided into two groups of 15 cows each. A basal diet was provided to the control group, and 30 g/(d-head) of NCG was added to the basal diet of the test group (NCG group). Blood samples were collected from the jugular vein after birth and before the end (when the calves were 90 days old) of the experiment for plasma metabolomics analysis. The metabolomics analysis identified 53 metabolites between the NCG group and control group, with 40 significantly up-regulated and 13 significantly down-regulated. Among them, 33 lipids and lipid-like molecules made up 57.89% of all the metabolites that were found. Thirty-three metabolic pathways enriched by metabolites showed p.adjust <0.05, among which glycerophospholipid and sphingolipid metabolism pathways were the most abundant. In conclusion, the addition of NCG in late-gestation cows appears to primarily affect calf growth and development through the regulation of phospholipid metabolism, which plays a role in nerve conduction, brain activity, and cell metabolism and function. This study provides valuable insights into how nutritional supplementation by late-gestation cows might improve the growth and development of newborn calves.
Active Disturbance-Rejection Controller (ADRC)-Based Torque Control for a Pneumatic Rotary Actuator with Positional Interference
Authors: Wei Qiong , Wu Zilong , Zhou Yue , Ke Ding , Zhang Daode
Published: February 2024

The compressibility of air, the uncertainty of dynamic models, and the existence of friction make pneumatic servo systems exhibit strong nonlinearity. Furthermore, the confluence of pneumatic-system nonlinearity and interference from the position system induces oscillations within the system, thereby posing a formidable challenge for achieving precise torque control. This study ensures precise torque control in a pneumatic actuator amid interference from the position system and proposes a novel active disturbance-rejection controller integrated with a Kalman filter. Firstly, in response to the oscillation stemming from the inherent nonlinearity of the pneumatic system and interference from the position system, this paper designs an active disturbance-rejection controller (ADRC) with robust anti-interference capabilities aimed at mitigating system oscillations. Secondly, to address the issue of sensor noise interfering with the ADRC and causing system oscillation, a first-order Kalman filter is designed to provide real-time and more accurate state estimation, effectively reducing oscillations and improving the robustness of the system. Finally, using the Lyapunov stability theory, the effectiveness of both the nonlinear extended observer and the convergence of the nonlinear error-state controller in the ADRC is proven. Experimental results indicate that the proposed controller reduces system oscillations and improves control accuracy.
New Tuning Rules of PI+CI Controllers for First-Order Systems
Authors: Moreno José Carlos , González José , Navarro Ana , Guzmán José Luis
Published: February 2024

The reset control is a simple nonlinear control approach where the states of the controller are conducted to zero when a particular condition is satisfied. The PI+CI is a controller that mixes the simplicity of PI controllers with the benefits of a reset action to mitigate the fundamental limitations of linear control. However, the tuning of this kind of controller, with three parameters, two for the linear part and one for the nonlinear one, is not trivial. In this paper, simple tuning rules for PI+CI are proposed for both tracking and regulation problems, assuming first-order dynamics for the plant. The resulting control scheme, for which the reset coefficient is computed from exponential functions, is simulated and compared with an ideal PI+CI where the reset coefficient is obtained using rules available in the literature. Similar results are obtained for the tracking problem, and optimal performance based on the Integral Absolute Error (IAE) is also obtained for the regulation problem. These new rules, in contrast to those already existing in the literature, depend only on closed-loop specifications. Furthermore, the framework based on the minimization of IAE, used to obtain the proposed rules, makes it possible to consider for the first time the tracking and regulation problems simultaneously, i.e., cases where setpoint changes and disturbance arrivals can occur at the same time before reaching a new steady state. The results are validated using a set of study cases.
Hormone Signals Involved in the Regulation of Cucumber Seedling Lateral Branch Elongation by Far-Red Light
Authors: Li Shuhao , Tian Jun , Ran Shengxiang , Zhou Yuqi , Gao Hongdou , Zhong Fenglin
Published: February 2024

Cucumber (Cucumis sativus L.) lateral branch elongation is influenced by a variety of environmental signals, including light [e.g., far-red (FR) light] and hormones. In this experiment, the effect of FR light on the lateral branch elongation of cucumber (‘Zhongnong No. 26’) seedlings was investigated. The results showed that FR light significantly inhibited the lateral branch elongation of cucumber seedlings. In addition, FR light significantly increased the auxin (indole-3-acetic acid, IAA) content, decreased the cytokinin (CTK; Zeatin) content, and suppressed the expression of most CTK synthetic-related genes, such as IPTs, in cucumber seedlings. The lateral branch elongation of cucumber seedlings was assessed in response to decapitation and exogenous 6-BA treatment to further investigate the relationship between IAA and CTK on the lateral branch elongation of cucumber seedlings under FR light. Both decapitation and exogenous 6-BA treatment eliminated the inhibitory effect of FR light on the lateral branch elongation of cucumber seedlings. In conclusion, these results indicated that IAA and CTK were involved in the regulatory effects of FR light on cucumber seedling lateral branch elongation.
Development of a Melting Curve-Based Triple Eva Green Real-Time PCR Assay for Simultaneous Detection of Three Shrimp Pathogens
Authors: Dong Xuan , Chen Yujin , Lou Haoyu , Wang Guohao , Zhou Chengyan , Wang Liying , Li Xuan , Luo Jingfei , Huang Jie
Published: February 2024

Infections with Enterocytozoon hepatopenaei (EHP), infectious hypodermal and hematopoietic necrosis virus (IHHNV), and Decapod iridescent virus 1 (DIV1) pose significant challenges to the shrimp industry. Here, a melting curve-based triple real-time PCR assay based on the fluorescent dye Eva Green was established for the simultaneous detection of EHP, IHHNV, and DIV1. The assay showed high specificity, sensitivity, and reproducibility. A total of 190 clinical samples from Shandong, Jiangsu, Sichuan, Guangdong, and Hainan provinces in China were evaluated by the triple Eva Green real-time PCR assay. The positive rates of EHP, IHHNV, and DIV1 were 10.5%, 18.9%, and 44.2%, respectively. The samples were also evaluated by TaqMan qPCR assays for EHP, DIV1, and IHHNV, and the concordance rate was 100%. This illustrated that the newly developed triple Eva Green real-time PCR assay can provide an accurate method for the simultaneous detection of three shrimp pathogens.
First Record of Kemp’s Ridley Sea Turtle, Lepidochelys kempii (Garman, 1880), in the Waters of Martinique Island (Lesser Antilles)
Authors: Chevallier Damien , Fretey Jacques , Lescure Jean , Girondot Marc
Published: February 2024

An adult female Lepidochelys kempii was found trapped in drifting sargassum south of Martinique; this is the southernmost report of this taxon in the Lesser Antilles arc. Determining the limits of distribution and the existence of possible sympatry between L. kempii and L. olivacea in certain subregions of the Caribbean has been hindered by numerous misidentifications. We review the available data and propose a new distribution map in the Caribbean, which can serve as a basis for future studies.
Bovine FRAS1: mRNA Expression Profile, Genetic Variations, and Significant Correlations with Ovarian Morphological Traits, Mature Follicle, and Corpus Luteum
Authors: Zhu Leijing , Shen Siyuan , Pan Chuanying , Lan Xianyong , Li Jie
Published: February 2024

The amelioration of bovine fertility caused by a multi-factorial problem has always been a hot topic, among which the detection of available target genes is the most crucial. It was hypothesized that the Fraser extracellular matrix complex subunit 1 (FRAS1) gene detected by GWAS is involved in physiological activities such as ovarian development. Herein, unilateral ovaries from 2111 cows were used to examine the mRNA expression profile and polymorphisms of bovine FRAS1 and their associations with fertility-related characteristics. Firstly, it was confirmed that FRAS1 gene transcripts are expressed in various bovine tissues. Then, among five potential insertion–deletion (indel) loci, the 20 bp (named P3-D20-bp) and 15 bp (P4-D15-bp) deletion mutations were confirmed to be polymorphic with linkage equilibrium. Secondly, the P3-D20-bp polymorphism was significantly associated with ovarian weight and corpus luteum diameter in the metaestrus phase and ovarian length in the dioestrum stage. Additionally, both ovarian length and mature follicle diameter in metaestrus are significantly correlated with different genotypes of P4-D15-bp. Thirdly, the transcriptional expression of the FRAS1 gene in groups with a minimum value of ovarian weight or volume was significantly higher than the expression in groups with a maximum value. Instead of that, the more corpus luteum and mature follicles there are, the higher the transcription expression of the FRAS1 gene is. Furthermore, FRAS1 expression in cows with a heterozygous genotype (ID) of P3-D20-bp was significantly higher than others. Eventually, P3-D20-bp deletion could disturb the binding efficiency of WT1-I and Sox2 to FRAS1 sequence according to binding prediction, indicating that mutation may affect gene expression and traits by influencing the binding of transcription factors. Overall, the polymorphisms of P3-D20-bp and P4-D15-bp of the bovine FRAS1 gene significantly correlated to follicle or ovarian traits that could be applied in optimizing female fertility in cow MAS breeding programs.
Retrospective Study of 25 Cases of Acorn Intoxication Colitis in Horses between 2011 and 2018 and Factors Associated with Non-Survival
Authors: Hermange Tanguy , Ruault Basile , Couroucé Anne
Published: February 2024

The aim of this study is to describe clinical data associated with acorn intoxication and to find variables associated with survival. Data from horses presented at CISCO-ONIRIS from 2011 to 2018 with a diagnosis of acorn intoxication were included. Diagnosis was based on the following: season, the presence of acorns in the environment, clinical and hemato-biochemical parameters suggestive of a digestive/renal disease, the co-morbidity of companion animals, and post-mortem findings. Statistical analysis was completed using Student’s t-test for mean comparisons and a Chi-square test for group comparisons (p < 0.05). A total of 25 horses were included, and seasonality suggests that the intoxication may vary from year to year. Clinical signs associated with acorn intoxication were signs of circulatory shock (lethargy, tachycardia, abnormal mucous membrane, tachypnea), digestive signs (diarrhea, ileus, colic), and abnormal temperature. Clinical pathological findings included increased hematocrit, WBC, creatinine, BUN, GGT, AST, CK and decreased albumin. Overall, 44% (11/25) of horses survived. The majority (13/14) of non-survivors died, or were euthanized, during the first 48 h. Findings significantly associated with non-survival were age, heart rate, hemorrhagic diarrhea, ileus, hematocrit, creatinine, blood lactate, and thickness of the colon wall at ultrasonography. This study provides equine practitioners with valuable prognostic information in cases of acorn intoxication.
Detection of Antibodies for Pathogenic Leptospira in Wild Mammals and Birds from Southern Chile—First Record of Seropositivity in a Guiña (Leopardus guigna)
Authors: Balcázar Luis , Azócar-Aedo Lucía , Barrera Violeta , Meniconi Gloria , Muñoz Victoria , Valencia-Soto Carola
Published: February 2024

Leptospirosis is a neglected bacterial zoonotic disease of worldwide distribution that is present in different animal species. This epidemiological study determined the seroprevalence of pathogenic Leptospira spp. in animals at a wildlife rehabilitation center in Puerto Montt, southern Chile, by sampling 60 animals belonging to three classes (birds, mammals, and reptiles). Diagnosis was performed using the microscopic agglutination test with a panel of eight serovars and serogroups. The results showed that 15 animals had anti-Leptospira antibodies, obtaining a seroprevalence of 25.00%, with Leptospira borgpetersenii serogroup Tarassovi presenting reactivity in 13 of the seropositive animals. Among the classes of mammals, chilla foxes (Lycalopex griseus) and pudus (Pudu puda) were seropositive. A guiña (Leopardus guigna) was also seropositive, which was described for the first time in mammals. Among the classes of birds, choroy parrots (Enicognathus leptorhynchus), bandurrias (Theristicus melanopis), and Magellanic penguins (Spheniscus magellanicus) were seropositive. Routine examinations to diagnose leptospirosis, perform epidemiological surveillance, and apply prevention and control measures are necessary, and additional research focusing on the One Health approach to explore the epidemiological role of different wild animal species in the maintenance and transmission of leptospirosis at the local and global levels are recommended.
Repurposing Mitomycin C in Combination with Pentamidine or Gentamicin to Treat Infections with Multi-Drug-Resistant (MDR) Pseudomonas aeruginosa
Authors: Svedholm Elin , Bruce Benjamin , Parcell Benjamin J. , Coote Peter J.
Published: February 2024

The aims of this study were (i) to determine if the combination of mitomycin C with pentamidine or existing antibiotics resulted in enhanced efficacy versus infections with MDR P. aeruginosa in vivo; and (ii) to determine if the doses of mitomycin C and pentamidine in combination can be reduced to levels that are non-toxic in humans but still retain antibacterial activity. Resistant clinical isolates of P. aeruginosa, a mutant strain over-expressing the MexAB-OprM resistance nodulation division (RND) efflux pump and a strain with three RND pumps deleted, were used. MIC assays indicated that all strains were sensitive to mitomycin C, but deletion of three RND pumps resulted in hypersensitivity and over-expression of MexAB-OprM caused some resistance. These results imply that mitomycin C is a substrate of the RND efflux pumps. Mitomycin C monotherapy successfully treated infected Galleria mellonella larvae, albeit at doses too high for human administration. Checkerboard and time–kill assays showed that the combination of mitomycin C with pentamidine, or the antibiotic gentamicin, resulted in synergistic inhibition of most P. aeruginosa strains in vitro. In vivo, administration of a combination therapy of mitomycin C with pentamidine, or gentamicin, to G. mellonella larvae infected with P. aeruginosa resulted in enhanced efficacy compared with monotherapies for the majority of MDR clinical isolates. Notably, the therapeutic benefit conferred by the combination therapy occurred with doses of mitomycin C close to those used in human medicine. Thus, repurposing mitomycin C in combination therapies to target MDR P. aeruginosa infections merits further investigation.
Collaborative Design of Static and Vibration Properties of a Novel Re-Entrant Honeycomb Metamaterial
Authors: Yong Jiawang , Dong Yiyao , Wan Zhishuai , Li Wanting , Chen Yanyan
Published: February 2024

A novel re-entrant honeycomb metamaterial based on 3D-printing technology is proposed by introducing chiral structures into diamond honeycomb metamaterial (DHM), named chiral-diamond-combined honeycomb metamaterial (CDCHM), and has been further optimized using the assembly idea. Compared with the traditional DHM, the CDCHM has better performance in static and vibration isolation. The static and vibration properties of the DHM and CDCHM are investigated by experiments and simulations. The results show that the CDCHM has a higher load-carrying capacity than that of the DHM. In addition, the vibration isolation optimal design schemes of the DHM and CDCHM are examined by experiments and simulations. It is found that the vibration suppression of the CDCHM is also improved greatly. In particular, the optimization approach with metal pins and particle damping achieves a wider bandgap in the low-frequency region, which can strengthen the suppression of low-frequency vibrations. And the introduction of particle damping can not only design the frequency of the bandgap via the alteration of the dosage, but also enhance the damping of the main structure. This work presents a new design idea for metamaterials, which provides a reference for the collaborative design of the static and vibration properties of composite metamaterials.
Antibiotic-Resistant Bacteria across a Wastewater Treatment Plant
Authors: Godinho Ofélia , Lage Olga Maria , Quinteira Sandra
Published: February 2024

Antimicrobial resistance is presently one of the leading causes of death worldwide. The surveillance of different environments, namely, wastewater treatment plants (WWTPs), as hotspots of antibiotic-resistant bacteria, has become crucial under the One Health approach. This study aimed to characterize, phenotypically and genotypically, antibiotic-resistant bacteria along a WWTP receiving domestic and industrial sewage. Four sampling sites, representing distinct treatment points of the WWTP, were selected for sampling bacterial isolation in selective media supplemented, or not, with antibiotics, and subsequent antimicrobial susceptibility testing. Antibiotic resistance encoding genes were screened by molecular methods. A total of 50 bacterial isolates were obtained, 50% of which were affiliated with the genus Enterococcus. The antimicrobial susceptibility testing revealed antibiotic phenotypic resistance in isolates obtained from all the four treatment points of the wastewater samples, with resistance to tetracycline (32.5%) and ampicillin (25%) being the most common. Three isolates were found to be multidrug resistant and were affiliated with the genera Citrobacter, Shigella and Klebsiella. Molecular screening revealed the presence of tet(M), blaTEM, blaSHV and blaCTX-M, as well as class 1 integrons carrying dfrA25, ANT(3″)-IIa and aadA6 genes. This study highlights the relevance of bacterial isolation and their antimicrobial susceptibility evaluation in WWTP systems since antibiotic-resistant strains were found from the raw influent to the final effluent discharged into the environment, denoting the need for surveillance and containment measures.
Beyond mirkwood: Enhancing SED Modeling with Conformal Predictions
Authors: Gilda Sankalp
Published: February 2024

Traditional spectral energy distribution (SED) fitting techniques face uncertainties due to assumptions in star formation histories and dust attenuation curves. We propose an advanced machine learning-based approach that enhances flexibility and uncertainty quantification in SED fitting. Unlike the fixed NGBoost model used in mirkwood, our approach allows for any scikit-learn-compatible model, including deterministic models. We incorporate conformalized quantile regression to convert point predictions into error bars, enhancing interpretability and reliability. Using CatBoost as the base predictor, we compare results with and without conformal prediction, demonstrating improved performance using metrics such as coverage and interval width. Our method offers a more versatile and accurate tool for deriving galaxy physical properties from observational data.
An Ensemble-Based Model for Specific Humidity Retrieval from Landsat-8 Satellite Data for South Korea
Authors: Choi Sungwon , Seong Noh-Hun , Jung Daeseong , Sim Suyoung , Woo Jongho , Kim Nayeon , Park Sungwoo , Han Kyung-soo
Published: February 2024

Specific humidity (SH) which means the amount of water vapor in 1 kg of air, is used as an indicator of energy exchange between the atmosphere and the Earth’s surface. SH is typically computed using microwave satellites. However, the spatial resolution of data for microwave satellite is too low. To overcome this disadvantage, we introduced new methods that applied data collected by the Landsat-8 satellite with high spatial resolution (30 m), a meteorological model, and observation data for South Korea in 2016–2017 to 4 machine learning techniques to develop an optimized technique for computing SH. Among the 4 machine learning techniques, the random forest-based method had the highest accuracy, with a coefficient of determination (R) of 0.98, Root Mean Square Error (RMSE) of 0.001, bias of 0, and Relative Root Mean Square Error (RRMSE) of 11.16%. We applied this model to compute land surface SH using data from 2018 to 2019 and found that it had high accuracy (R = 0.927, RMSE = 0.002, bias = 0, RRMSE = 28.35%). Although the data used in this study were limited, the model was able to accurately represent a small region based on an ensemble of satellite and model data, demonstrating its potential to address important issues related to SH measurements from satellites.
Intra-Articular Injection of Adipose-Derived Stromal Vascular Fraction in Osteoarthritic Temporomandibular Joints: Study Design of a Randomized Controlled Clinical Trial
Authors: Schipper Jan Aart M. , Tuin Aartje Jorien , van Dongen Joris A. , van Bakelen Nico B. , Harmsen Martin Conrad , Spijkervet Fred K. L.
Published: February 2024

Introduction: Temporomandibular joint (TMJ) osteoarthritis is a degenerative disease of the TMJ. It is characterized by progressive degradation of the extracellular matrix components of articular cartilage, with secondary inflammatory components leading to pain in the temporomandibular region and reduced mouth opening. Current treatments do not halt disease progression, hence the need for new therapies to reduce inflammation and, consequently, improve symptoms. The aim of our randomized controlled clinical trial protocol is to investigate the efficacy of adjuvant intra-articular injections of autologous tissue-like stromal vascular fraction (tSVF), compared to arthrocentesis alone, in reducing pain and improving mouth opening in TMJ osteoarthritis patients. Materials and Methods: The primary endpoint analysis will consist of the visual analogue scale (VAS) for pain. The secondary endpoint analyses will include maximal interincisal mouth opening measurements; assessment of oral health and mandibular function based on the oral health impact profile (OHIP) questionnaire and mandibular functional impairment questionnaire (MFIQ); complications during the follow up; synovial cytokine analysis at baseline and after 26 weeks; and nucleated cells and tSVF (immuno)histochemistry analyses of the intervention group. Discussion: Our randomized clinical trial protocol will be applied to evaluate the efficacy of a new promising tSVF injection therapy for TMJ osteoarthritis. The safety of intra-articular injections of tSVF has been proven for knee osteoarthritis. However, since a tSVF injection is considered a heterologous application of cell therapy, the regulatory requirements are strict, which makes medical ethical approval challenging.
Influence of Backpack Carriage and Walking Speed on Muscle Synergies in Healthy Children
Authors: Marino Giorgia , Scano Alessandro , Beltrame Giulia , Brambilla Cristina , Marazzi Alessandro , Aparo Francesco , Molinari Tosatti Lorenzo , Gatti Roberto , Portinaro Nicola
Published: February 2024

Four to five muscle synergies account for children’s locomotion and appear to be consistent across alterations in speed and slopes. Backpack carriage induces alterations in gait kinematics in healthy children, raising questions regarding the clinical consequences related to orthopedic and neurological diseases and ergonomics. However, to support clinical decisions and characterize backpack carriage, muscle synergies can help with understanding the alterations induced in this condition at the motor control level. In this study, we investigated how children adjust the recruitment of motor patterns during locomotion, when greater muscular demands are required (backpack carriage). Twenty healthy male children underwent an instrumental gait analysis and muscle synergies extraction during three walking conditions: self-selected, fast and load conditions. In the fast condition, a reduction in the number of synergies (three to four) was needed for reconstructing the EMG signal with the same accuracy as in the other conditions (three to five). Synergies were grouped in only four clusters in the fast condition, while five clusters were needed for the self-selected condition. The right number of clusters was not clearly identified in the load condition. Speed and backpack carriage altered nearly every spatial–temporal parameter of gait, whereas kinematic alterations reflected mainly hip and pelvis adaptations. Although the synergistic patterns were consistent across conditions, indicating a similar motor pattern in different conditions, the fast condition required fewer synergies for reconstructing the EMG signal with the same level of accuracy.
Ultrasound Flow Imaging Study on Rat Brain with Ultrasound and Light Stimulations
Authors: Zhang Junhang , Gong Chen , Yang Zihan , Wei Fan , Sun Xin , Ji Jie , Zeng Yushun , Chang Chi-feng , Liu Xunan , Nair Deepthi S. Rajendran , Thomas Biju B. , Zhou Qifa
Published: February 2024

Functional ultrasound (fUS) flow imaging provides a non-invasive method for the in vivo study of cerebral blood flow and neural activity. This study used functional flow imaging to investigate rat brain’s response to ultrasound and colored-light stimuli. Male Long-Evan rats were exposed to direct full-field strobe flashes light and ultrasound stimulation to their retinas, while brain activity was measured using high-frequency ultrasound imaging. Our study found that light stimuli, particularly blue light, elicited strong responses in the visual cortex and lateral geniculate nucleus (LGN), as evidenced by changes in cerebral blood volume (CBV). In contrast, ultrasound stimulation elicited responses undetectable with fUS flow imaging, although these were observable when directly measuring the brain’s electrical signals. These findings suggest that fUS flow imaging can effectively differentiate neural responses to visual stimuli, with potential applications in understanding visual processing and developing new diagnostic tools.
Cytoskeletal Protein 4.1R in Health and Diseases
Authors: Liu Jiaojiao , Ding Cong , Liu Xin , Kang Qiaozhen
Published: February 2024

The protein 4.1R is an essential component of the erythrocyte membrane skeleton, serving as a key structural element and contributing to the regulation of the membrane’s physical properties, including mechanical stability and deformability, through its interaction with spectrin–actin. Recent research has uncovered additional roles of 4.1R beyond its function as a linker between the plasma membrane and the membrane skeleton. It has been found to play a crucial role in various biological processes, such as cell fate determination, cell cycle regulation, cell proliferation, and cell motility. Additionally, 4.1R has been implicated in cancer, with numerous studies demonstrating its potential as a diagnostic and prognostic biomarker for tumors. In this review, we provide an updated overview of the gene and protein structure of 4.1R, as well as its cellular functions in both physiological and pathological contexts.
Tumor Microenvironment-Responsive Drug Delivery Based on Polymeric Micelles for Precision Cancer Therapy: Strategies and Prospects
Authors: Jin Zhu , Al Amili Majdi , Guo Shengrong
Published: February 2024

In clinical practice, drug therapy for cancer is still limited by its inefficiency and high toxicity. For precision therapy, various drug delivery systems, including polymeric micelles self-assembled from amphiphilic polymeric materials, have been developed to achieve tumor-targeting drug delivery. Considering the characteristics of the pathophysiological environment at the drug target site, the design, synthesis, or modification of environmentally responsive polymeric materials has become a crucial strategy for drug-targeted delivery. In comparison to the normal physiological environment, tumors possess a unique microenvironment, characterized by a low pH, high reactive oxygen species concentration, hypoxia, and distinct enzyme systems, providing various stimuli for the environmentally responsive design of polymeric micelles. Polymeric micelles with tumor microenvironment (TME)-responsive characteristics have shown significant improvement in precision therapy for cancer treatment. This review mainly outlines the most promising strategies available for exploiting the tumor microenvironment to construct internal stimulus-responsive drug delivery micelles that target tumors and achieve enhanced antitumor efficacy. In addition, the prospects of TME-responsive polymeric micelles for gene therapy and immunotherapy, the most popular current cancer treatments, are also discussed. TME-responsive drug delivery via polymeric micelles will be an efficient and robust approach for developing clinical cancer therapies in the future.
Natural Killer Cells as a Further Insight into the Course of Chronic Obstructive Pulmonary Disease
Authors: Brajer-Luftmann Beata , Trafas Tomasz , Stelmach-Mardas Marta , Bendowska Weronika , Piorunek Tomasz , Grabicki Marcin , Kaczmarek Mariusz
Published: February 2024

The role of natural killer (NK) cells in chronic obstructive pulmonary disease (COPD) pathogenesis has been discussed but is not yet clearly understood. This current study aimed to evaluate the associations between immunophenotypes, degrees of maturity, and the expression level of functional receptors of NK cells in the lung environment present in bronchoalveolar lavage fluid (BALF), and an attempt was made to determine their relationship in the course and progression of COPD. A total of 15 COPD patients and 14 healthy smokers were included. The clinical parameters of COPD were evaluated. In both groups, NK cells using monoclonal antibodies directly conjugated with fluorochromes in flow cytometry were assessed in the peripheral blood. Additionally, NK cells using the same method were assessed in BALF in the COPD subgroup. The blood’s NK cells differed from the estimated group’s maturity and receptor expression. Functional receptors CD158b+, CD314+, and CD336+ expressed by NK cells were significantly interlinked with age, RV, TLC, 6MWT, smoking, and the number of exacerbations. These results confirm the essential role of NK cells in COPD pathogenesis. Additionally, the relationship between clinical parameters and NK cell expression may indicate its participation in the disease progression and exacerbation and allow for a better understanding of NK cell biology in COPD.
Effect of the COVID-19 Pandemic on Resting-State Brain Activity in Individuals with Tinnitus
Authors: Jedrzejczak W. Wiktor , Gos Elżbieta , Ganc Malgorzata , Raj-Koziak Danuta , Skarzynski Piotr H. , Skarzynski Henryk
Published: February 2024

This study looked at the possible effect of the COVID-19 pandemic on individuals who came to our clinic seeking relief from tinnitus. The performance of the subjects during the COVID-19 pandemic was compared with similar individuals who came to our clinic before the pandemic began. The study involved 50 adults with chronic tinnitus, made up of a study group (24 subjects tested during the COVID-19 pandemic of 2020–2021) and a control group before the pandemic began (26 subjects tested from 2013 to 2017). None of the 24 reported having contracted COVID-19. Data collection involved the Tinnitus Handicap Inventory (THI) questionnaire, audiological tests, and quantitative electroencephalography (qEEG). In terms of THI scores, there were no statistically significant differences between the two groups. However, with regard to qEEG, some changes were observed, with significant decreases in alpha and beta band activity in the study group compared to the control group, particularly over the auditory cortex. We conclude that COVID-19 did not have a discernible impact on the general well-being of individuals with tinnitus. However, it did appear to alter brain activity, specifically in the alpha and beta bands over the auditory cortex, and these reults warrant further investigation.
Application of Novel Phase Change Material Constructive Solution for Thermal Regulation of Passive Solar Buildings
Authors: Figueiredo António , Silva Tiago , Gonçalves Margarida , Samagaio António
Published: February 2024

A comprehensive investigation regarding the hygrothermal behavior of a constructive solution containing phase change materials (PCMs) was performed on a full-scale test cell, divided into two similar compartments. This involved hygrothermal monitoring (indoor air temperature) of the two compartments, in which one had PCM incorporated into the floor mortar. The main goal of this research was to investigate the potential of this kind of solution for overheating mitigation. The numerical study was conducted using EnergyPlus® software (version 9.0), exploring different natural ventilation flow rates to gauge the novel solution’s potential to reduce overheating rates. The results from the monitoring studies revealed prolonged periods of thermal discomfort in both test cells, particularly overheating. However, it was proven that the PCM application in one of the test cells led to a reduction of almost 10 °C in the maximum peak of air temperature. In the simulation analysis, the increase in the ventilation rate led to a linear decrease in the overheating hours of up to one renovation per hour, and then the reductions were attenuated.
Study on the Influence and Optimization Design of Viscous Damper Parameters on the Damping Efficiency of Frame Shear Wall Structure
Authors: Lan Xiang , Wei Guanglan , Zhang Xingxian
Published: February 2024

This study investigates the influence of viscous damper parameters on the damping efficiency of frame shear wall structures. Taking a specific frame shear wall structure as the background, a three-dimensional finite element model is established using a nonlinear dynamic time–history analysis method. The damping ratio, reduction in vertex displacement, reduction in base shear, and inter-story drift utilization rate are selected as the damping performance indicators. Firstly, a sensitivity analysis is conducted to study the influence of different viscous damper parameters on these indicators. Then, the relationship models between the viscous damper parameters and the indicators are fitted using the response surface method, and the fitting effect is evaluated through an F-test and determination coefficient R2. Finally, an objective function based on key damping performance indicators is established to solve for the optimal parameters. The results show that the traditional sensitivity analysis method is unable to comprehensively consider the combined effects of different damping efficiency indicators. The response surface method has high fitting accuracy and good predictability and can serve as an optimization model. Considering the stiffness of supporting components matched with the viscous damper parameters, the feasibility of the optimal damping parameters is demonstrated from an engineering application perspective. A simple and easy-to-operate damping design flowchart is proposed, providing important guidance and reference for designers in frame shear wall structure damping design in the future.
Subtype-Specific Survival of Young Women with Breast Cancer and Its Interaction with the Germline BRCA Status
Authors: Hage Anna Maria , Gebert Pimrapat , Blohmer Jens-Uwe , Hedayati Elham , Speiser Dorothee , Karsten Maria Margarete
Published: February 2024

Data are scarce on the role of pathogenic germline variants in BRCA1 and BRCA2 (gBRCAm) in subtype-specific survival in young women who develop breast cancer under the age of 40. This retrospective, real-world cohort study assessed the distant disease-free survival (DDFS) and overall survival (OS) of young women diagnosed with breast cancer between 2008 and 2019 while taking into consideration the interaction of clinical subtypes and the gBRCA status. Among 473 women, HR+/Her2− was the most common subtype (49.0%), followed by TNBC (31.3%), HR+/Her2+ (13.7%), and Her2+/HR− (5.9%). The gBRCA status was known for 319 cases (gBRCAwt (wild-type – without pathogenic variants in BRCA1 or BRCA2): 204, gBRCA1m: 83, gBRCA2m: 31, 1 patient with both). The distribution of clinical subtypes varied depending on the gBRCA status (p < 0.001). In survival analysis with a median follow-up of 43 months, the unadjusted DDFS and OS were worse for gBRCAwt TNBC compared to both HR+ subtypes, but not for gBRCAm TNBC patients. T-stage, nodal involvement, and the gBRCA status were identified as significant for survival in TNBC. In TNBC, gBRCAm was associated with better DDFS and OS than gBRCAwt (5-year DDFS 81.4% vs. 54.3%, p = 0.012 and 5-year OS 96.7% vs. 62.7%, p < 0.001). In contrast, in HR+/Her2− patients, gBRCAm patients showed a tendency for worse survival, though not statistically significant. Subtype-specific survival in young women with breast cancer needs to be evaluated in interaction with the gBRCA status. For TNBC, gBRCAm is of favorable prognostic value for overall survival, while patients with gBRCAwt TNBC need to be considered to have the highest risk for adverse survival outcomes.
Theranostic Uses of the Heme Pathway in Neuro-Oncology: Protoporphyrin IX (PpIX) and Its Journey from Photodynamic Therapy (PDT) through Photodynamic Diagnosis (PDD) to Sonodynamic Therapy (SDT)
Authors: Marcus Stuart L. , de Souza Mark P.
Published: February 2024

ALA PDT, first approved as a topical therapy to treat precancerous skin lesions in 1999, targets the heme pathway selectively in cancers. When provided with excess ALA, the fluorescent photosensitizer PpIX accumulates primarily in cancer tissue, and ALA PDD is used to identify bladder and brain cancers as a visual aid for surgical resection. ALA PDT has shown promising anecdotal clinical results in recurrent glioblastoma multiforme. ALA SDT represents a noninvasive way to activate ALA PDT and has the potential to achieve clinical success in the treatment of both intracranial and extracranial cancers. This review describes the creation and evolution of ALA PDT, from the treatment of skin cancers to PDD and PDT of malignant brain tumors and, most recently, into a noninvasive form of PDT, ALA SDT. Current clinical trials of ALA SDT for recurrent glioblastoma and high-grade gliomas in adults, and the first pediatric ALA SDT clinical trial for a lethal brainstem cancer, diffuse intrinsic pontine glioma (DIPG), are also described.