bluuebunny/crossref_metadata_2025 huggingface dataset:
Musashi-1 and miR-147 Precursor Interaction Mediates Synergistic Oncogenicity Induced by Co-Infection of Two Avian Retroviruses
Authors: Zhou Defang , Ding Longying , Xu Menglu , Liu Xiaoyao , Xue Jingwen , Zhang Xinyue , Du Xusheng , Zhou Jing , Cui Xiyao , Cheng Ziqiang
Published: October 2022

Synergism between avian leukosis virus subgroup J (ALV-J) and reticuloendotheliosis virus (REV) has been reported frequently in co-infected chicken flocks. Although significant progress has been made in understanding the tumorigenesis mechanisms of ALV and REV, how these two simple oncogenic retroviruses induce synergistic oncogenicity remains unclear. In this study, we found that ALV-J and REV synergistically promoted mutual replication, suppressed cellular senescence, and activated epithelial-mesenchymal transition (EMT) in vitro. Mechanistically, structural proteins from ALV-J and REV synergistically activated the expression of Musashi-1(MSI1), which directly targeted pri-miR-147 through its RNA binding site. This inhibited the maturation of miR-147, which relieved the inhibition of NF-κB/KIAA1199/EGFR signaling, thereby suppressing cellular senescence and activating EMT. We revealed a synergistic oncogenicity mechanism induced by ALV-J and REV in vitro. The elucidation of the synergistic oncogenicity of these two simple retroviruses could help in understanding the mechanism of tumorigenesis in ALV-J and REV co-infection and help identify promising molecular targets and key obstacles for the joint control of ALV-J and REV and the development of clinical technologies.
Low Nephron Number Induced by Maternal Protein Restriction Is Prevented by Nicotinamide Riboside Supplementation Depending on Sirtuin 3 Activation
Authors: Pezzotta Anna , Perico Luca , Morigi Marina , Corna Daniela , Locatelli Monica , Zoja Carlamaria , Benigni Ariela , Remuzzi Giuseppe , Imberti Barbara
Published: October 2022

A reduced nephron number at birth, due to critical gestational conditions, including maternal malnutrition, is associated with the risk of developing hypertension and chronic kidney disease in adulthood. No interventions are currently available to augment nephron number. We have recently shown that sirtuin 3 (SIRT3) has an important role in dictating proper nephron endowment. The present study explored whether SIRT3 stimulation, by means of supplementation with nicotinamide riboside (NR), a precursor of the SIRT3 co-substrate nicotinamide adenine dinucleotide (NAD+), was able to improve nephron number in a murine model of a low protein (LP) diet. Our findings show that reduced nephron number in newborn mice (day 1) born to mothers fed a LP diet was associated with impaired renal SIRT3 expression, which was restored through supplementation with NR. Glomerular podocyte density, as well as the rarefaction of renal capillaries, also improved through NR administration. In mechanistic terms, the restoration of SIRT3 expression through NR was mediated by the induction of proliferator-activated receptor γ (PPARγ) coactivator-1α (PGC-1α). Moreover, NR restored SIRT3 activity, as shown by the reduction of the acetylation of optic atrophy 1 (OPA1) and superoxide dismutase 2 (SOD2), which resulted in improved mitochondrial morphology and protection against oxidative damage in mice born to mothers fed the LP diet. Our results provide evidence that it is feasible to prevent nephron mass shortage at birth through SIRT3 boosting during nephrogenesis, thus providing a therapeutic option to possibly limit the long-term sequelae of reduced nephron number in adulthood.
β-COP Regulates TWIK1/TREK1 Heterodimeric Channel-Mediated Passive Conductance in Astrocytes
Authors: Kim Seong-Seop , Bae Yeonju , Kwon Osung , Kwon Seung-Hae , Seo Jong Bok , Hwang Eun Mi , Park Jae-Yong
Published: October 2022

Mature astrocytes are characterized by a K+ conductance (passive conductance) that changes with a constant slope with voltage, which is involved in K+ homeostasis in the brain. Recently, we reported that the tandem of pore domains in a weak inward rectifying K+ channel (TWIK1 or KCNK1) and TWIK-related K+ channel 1 (TREK1 or KCNK2) form heterodimeric channels that mediate passive conductance in astrocytes. However, little is known about the binding proteins that regulate the function of the TWIK1/TREK1 heterodimeric channels. Here, we found that β-coat protein (COP) regulated the surface expression and activity of the TWIK1/TREK1 heterodimeric channels in astrocytes. β-COP binds directly to TREK1 but not TWIK1 in a heterologous expression system. However, β-COP also interacts with the TWIK1/TREK1 heterodimeric channel in a TREK1 dependent manner and enhances the surface expression of the heterodimeric channel in astrocytes. Consequently, it regulates TWIK1/TREK1 heterodimeric channel-mediated passive conductance in astrocytes in the mouse brain. Taken together, these results suggest that β-COP is a potential regulator of astrocytic passive conductance in the brain.
Synovial Macrophages Expression of OX40L Is Required for Follicular Helper T Cells Differentiation in the Joint Microenvironment
Authors: Cai Xiaoyu , Zhang Meng , Ren Fujia , Fei Weidong , Zhang Xiao , Zhao Yunchun , Yao Yao , Lin Nengming
Published: October 2022

Signaling via the OX40/OX40L axis plays a key role in CD4+ T cell development, and OX40L expression is primarily restricted to antigen-presenting cells (APCs). This study was designed to assess the role of APC-mediated OX40L expression in the context of the development of rheumatoid arthritis (RA)-associated CD4+ T cell subsets. For these analyses, clinical samples were harvested from patients with osteoarthritis and RA, with additional analyses performed using OX40−/− mice and mice harboring monocyte/macrophage-specific deletions of OX40L. Together, these analyses revealed tissue-specific roles for OX40/OX40L signaling in RA. Specifically, higher levels of synovial macrophage OX40L expression were associated with the enhanced development of T follicular helper cells in the joint microenvironment, thereby contributing to the pathogenesis of RA. This Tfh differentiation was found to be OX40/OX40L-dependent in this synovial setting. Overall, these results indicate that the expression of OX40L by synovia macrophages is necessary to support Tfh differentiation in the joint tissues, thus offering new insight regarding the etiological basis for RA progression.
mRNA-Based Approaches to Treating Liver Diseases
Authors: Cacicedo Maximiliano L. , Limeres María José , Gehring Stephan
Published: October 2022

Diseases that affect the liver account for approximately 2 million deaths worldwide each year. The increasing prevalence of these diseases and the limited efficacy of current treatments are expected to stimulate substantial growth in the global market for therapeutics that target the liver. Currently, liver transplantation is the only curative option available for many liver diseases. Gene therapy represents a valuable approach to treatment. The liver plays a central role in a myriad of essential metabolic functions, making it an attractive organ for gene therapy; hepatocytes comprise the most relevant target. To date, viral vectors constitute the preferred approach to targeting hepatocytes with genes of therapeutic interest. Alternatively, mRNA-based therapy offers a number of comparative advantages. Clinical and preclinical studies undertaken to treat inherited metabolic diseases affecting the liver, cirrhosis and fibrosis, hepatocellular carcinoma, hepatitis B, and cytomegalovirus using lipid nanoparticle-encapsulated mRNAs that encode the therapeutic or antigenic protein of interest are discussed.
C/EBPδ Suppresses Motility-Associated Gene Signatures and Reduces PDAC Cell Migration
Authors: Hartl Leonie , Maarschalkerweerd Pien A. F. , Butler Joe M. , Manz Xue D. , Thijssen Victor L. J. L. , Bijlsma Maarten F. , Duitman JanWillem , Spek C. Arnold
Published: October 2022

Pancreatic Ductal Adenocarcinoma (PDAC) is among the most aggressive human cancers and occurs globally at an increasing incidence. Metastases are the primary cause of cancer-related death and, in the majority of cases, PDAC is accompanied by metastatic disease at the time of diagnosis, making it a particularly lethal cancer. Regrettably, to date, no curative treatment has been developed for patients with metastatic disease, resulting in a 5-year survival rate of only 11%. We previously found that the protein expression of the transcription factor CCAAT/Enhancer-Binding Protein Delta (C/EBPδ) negatively correlates with lymph node involvement in PDAC patients. To better comprehend the etiology of metastatic PDAC, we explored the role of C/EBPδ at different steps of the metastatic cascade, using established in vitro models. We found that C/EBPδ has a major impact on cell motility, an important prerequisite for tumor cells to leave the primary tumor and to reach distant sites. Our data suggest that C/EBPδ induces downstream pathways that modulate actin cytoskeleton dynamics to reduce cell migration and to induce a more epithelial-like cellular phenotype. Understanding the mechanisms dictating epithelial and mesenchymal features holds great promise for improving the treatment of PDAC.
The Proteostasis Network: A Global Therapeutic Target for Neuroprotection after Spinal Cord Injury
Authors: Whittemore Scott R. , Saraswat Ohri Sujata , Forston Michael D. , Wei George Z. , Hetman Michal
Published: October 2022

Proteostasis (protein homeostasis) is critical for cellular as well as organismal survival. It is strictly regulated by multiple conserved pathways including the ubiquitin-proteasome system, autophagy, the heat shock response, the integrated stress response, and the unfolded protein response. These overlapping proteostasis maintenance modules respond to various forms of cellular stress as well as organismal injury. While proteostasis restoration and ultimately organism survival is the main evolutionary driver of such a regulation, unresolved disruption of proteostasis may engage pro-apoptotic mediators of those pathways to eliminate defective cells. In this review, we discuss proteostasis contributions to the pathogenesis of traumatic spinal cord injury (SCI). Most published reports focused on the role of proteostasis networks in acute/sub-acute tissue damage post-SCI. Those reports reveal a complex picture with cell type- and/or proteostasis mediator-specific effects on loss of neurons and/or glia that often translate into the corresponding modulation of functional recovery. Effects of proteostasis networks on such phenomena as neuro-repair, post-injury plasticity, as well as systemic manifestations of SCI including dysregulation of the immune system, metabolism or cardiovascular function are currently understudied. However, as potential interventions that target the proteostasis networks are expected to impact many cell types across multiple organ systems that are compromised after SCI, such therapies could produce beneficial effects across the wide spectrum of highly variable human SCI.
Ferroptosis: A New Development Trend in Periodontitis
Authors: Chen Kexiao , Ma Shuyuan , Deng Jianwen , Jiang Xinrong , Ma Fengyu , Li Zejian
Published: October 2022

Periodontitis is a chronic inflammatory disease associated with bacterial biofilm. It is characterized by loss of periodontal support tissue and has long been considered as a “silent disease”. Because it is difficult to prevent and has a health impact that can not be ignored, researchers have been focusing on a mechanism-based treatment model. Ferroptosis is an iron-dependent regulatory form of cell death, that directly or indirectly affects glutathione peroxidase through different signaling pathways, resulting in a decrease in cell antioxidant capacity, accumulation of reactive oxygen species and lipid peroxidation, which cause oxidative cell death and tissue damage. Recently, some studies have proven that iron overload, oxidative stress, and lipid peroxidation exist in the process of periodontitis. Based on this, this article reviews the relationship between periodontitis and ferroptosis, in order to provide a theoretical reference for future research on the prevention and treatment of periodontal disease.
In Vitro Detection of Biochemical Effect in Human CaCo-2 Cell Line after Exposure to a Low Concentration of a Deltamethrin-Based Pesticide
Authors: Perna Giuseppe , Capozzi Vito , Lasalvia Maria
Published: October 2022

Pesticide residues are chemicals frequently found in food as contaminants. Pesticides may have adverse health effects, particularly when the digestive tract is concerned, as a consequence of food ingestion. Deltamethrin is a pyrethroid pesticide widely used in various fields, such as agriculture, veterinary and in the household, so the ingestion of a small amount of this chemical may occasionally occur. To assess whether exposure to pesticide residues may have a biological effect at the intestinal level, it is primarily necessary to perform in vitro exposure experiments about cell lines models of the intestinal barrier at low concentrations of the chemical. In the present study, CaCo-2 cells were exposed to different concentrations of a Deltamethrin-based commercial pesticide, which was diluted in the cell medium. An MTT viability test indicated that the cytotoxic concentration value of the pesticide inside 1 mL of medium is between 10−6 and 10−5 mL. However, the analysis of Raman spectra found that biochemical changes occur inside cells exposed to a non-cytotoxic concentration of 10−6 mL of the pesticide inside 1 mL of the medium. Such changes involve mainly an increase in the ratio between the amount of lipid with respect to that of the protein components in the cell cytoplasm. The results obtained by Raman micro-spectroscopy were confirmed by fluorescence images obtained by using a fluorophore staining neutral lipids. Overall, the obtained results suggest that Raman micro-spectroscopy can be successfully used to monitor the cellular modifications due to exposure at low concentrations of pesticides, as those values that can be found inside food are residuals.
Sudden Death in Patients with a History of Kawasaki Disease under School Supervision
Authors: Ayusawa Mamoru , Namiki Hidemasa , Abe Yuriko , Ichikawa Rie , Morioka Ichiro
Published: October 2022

We investigated the incidence of sudden death in students with a history of Kawasaki disease (KD) while under school supervision. Reports of sudden death in students with a history of KD during 1990–1999 and 2000–2009 were retrieved from the mutual aid system data. The student’s grade, sex, circumstances at the time of sudden death, final diagnosis, recommended restrictions on school activities, and intensity of physical activity at the time of sudden death were investigated. There were 11 cases from 1990 to 1999 and 3 from 2000 to 2009; KD was complicated with coronary artery aneurysm (CAA) in nine and one cases, respectively. The incidence of sudden death decreased by approximately 50% for KD history and 80% for KD with CAA between the two decades; however, the difference was not statistically significant. Of the 14 cases, 12 occurred during moderate-to-strenuous exercise; the restriction on exercise for students with KD complicated with CAA was not followed in at least five cases during 1990–1999, while three cases during 2000–2009 occurred without recommended restriction. Cases of sudden cardiac death decreased during 2000–2009, compared with those during 1990–1999. Special attention is required for students with a history of KD, particularly when complicated with CAA.
Clinical Characteristics and Prognosis of the Modified Probable Pneumocystis jirovecii Pneumonia in Korean Children, 2001–2021
Authors: Yun Kyoung Sung , Anh Bin , Choi Sung Hwan , Hong Kyung Taek , Choi Jung Yoon , Yun Ki Wook , Kang Hyoung Jin , Choi Eun Hwa
Published: October 2022

There are few data about Pneumocystis jirovecii pneumonia (PCP) in children, particularly in developed countries. This study investigated the clinical characteristics and prognosis of the clinical PCP in non-HIV-infected Korean children. Children with positive results for the staining and/or polymerase chain reaction (PCR) for P. jirovecii between 2001 and 2021 were identified. Patients were grouped into clinical PCP, which comprised proven and modified probable cases, and non-PCP groups. Modified probable PCP (mp-PCP) indicate the case which P. jirovecii was detected by conventional PCR rather than real-time PCR test. The differences in demographic and clinical characteristics were analyzed between the groups. A total of 110 pneumonia cases with positive results for P. jirovecii PCR and/or stain were identified from 107 children. Of these, 28.2% were classified as non-PCP, 12.7% of proven PCP, and 59.1% of mp-PCP. Compared with the non-PCP group, the mp-PCP group had a significantly higher rate of solid organ transplantation (3.2% vs. 24.6%), fever (58.1% vs. 76.9%), tachypnea (25.8% vs. 66.2%), dyspnea (48.4% vs. 83.1%), desaturation (48.4% vs. 80.0%), and bilateral ground-glass opacity on chest radiograph (19.4% vs. 73.8%). However, when the mp-PCP group was compared with the proven PCP group, there was no statistically significant difference. For children with clinical PCP, age under 5 years of age (odds ratio [OR] 10.7), hospital-onset (OR 6.9), and desaturation as initial symptom (OR 63.5) were significant risk factors for death in multivariable analysis. Modified probable PCP might reliably reflect true PCP in terms of patient’s demographic, clinical features, treatment response, and prognosis. Immunocompromised children with hospital-onset pneumonia who are younger than 5 years of age and have desaturation would be more cautiously and aggressively managed for survival through the screening for P. jirovecii by conventional PCR on appropriate lower respiratory specimens.
Positive Attention Bias Trained during the Rethink Therapeutic Online Game and Related Improvements in Children and Adolescents’ Mental Health
Authors: David Oana A. , Magurean Silvia
Published: October 2022

Attentional bias towards positive stimuli is considered a resilience factor for mental health and well-being. The aim of the present study was to analyze the effects of an attentional bias training for positive faces in a preventive therapeutic game for children and adolescents. The sample of 54, which consisted of children and adolescents aged between 10–16 years, played the REThink game, which included an attentional bias training level based on the visual search paradigm, where children had the task to quickly find the happy face among other angry faces. We measured mental health, and positive and negative emotions and analyzed their associations between changes in attention bias. Attentional bias indicators demonstrated acceptable reliability and results showed that increases in attentional bias towards positive faces were associated with improvements in children and adolescents’ conduct problems, hyperactivity, and peer relationship problems. Overall, our results support the protective role of training attentional bias towards positive faces as part of a preventive therapeutic game for children and adolescents.
Bullying Victimization Moderates the Association between Social Skills and Self-Esteem among Adolescents: A Cross-Sectional Study in International Schools
Authors: Dou Yunru , Wongpakaran Tinakon , Wongpakaran Nahathai , O’Donnell Ronald , Bunyachatakul Saifon , Pojanapotha Pichaya
Published: October 2022

Background. Bullying is a major school problem. Victims of bullying often experience low self-esteem, whereas social skills are positively associated with the level of self-esteem. This research examined whether the victim’s condition impacted their social skills and self-esteem. Methods. International school students in Thailand aged 13 to 18 years old completed the Olweus bullying questionnaire, social capital questionnaire (SC), social skills questionnaire (SS), adolescent discrimination index (ADDI), and the Rosenberg self-esteem scale (RSES). Moderation analyses and visual presentations were carried out using IBM SPSS ver. 22 and PROCESS, ver. 4.0. Results. A total of 102 students participated (63% female). The mean age of the participants was 16.57 (SD = 1.42). The number of victims was 16 (15.7%), the mean (SD) for the SC, SS, ADDI, and RSES was 7.82 (2.37), 44.45 (9.40), 12.33 (9.82), and 27.85 (5.31), respectively. As predicted, those with high social skills reported greater self-esteem when they had never been bullied. The moderation effect was significant: B = 0.458, standard error = 0.203, 95% CI = −0.836 to −0.054. Additionally, the ADDI and SC were found to predict self-esteem. Conclusions. The significant moderation effect suggests the importance of identifying the victim’s condition when the association between social skills and self-esteem is not observed (as expected) among school adolescents. A longitudinal study to confirm the causal relationship should be encouraged. Further research on providing appropriate interventions along with social skill training for the victim group is warranted.
Central Venous Catheter Cannulation in Pediatric Anesthesia and Intensive Care: A Prospective Observational Trial
Authors: Vafek Václav , Skříšovská Tamara , Kosinová Martina , Klabusayová Eva , Musilová Tereza , Kramplová Tereza , Djakow Jana , Klučka Jozef , Kalina Jiří , Štourač Petr
Published: October 2022

Currently, ultrasound-guided central venous catheter (CVC) insertion is recommended in pediatric patients. However, the clinical practice may vary. The primary aim of this study was the overall success rate and the first attempt success rate in ultrasound-guided CVC insertion versus anatomic-based CVC insertion in pediatric patients. The secondary aim was the incidence of associated complications and the procedural time. The physician could freely choose the cannulation method and venous approach. Data were collected for 10 months. Overall, 179 patients were assessed for eligibility and 107 patients were included. In almost half of the patients (48.6%), the percutaneous puncture was performed by real-time ultrasound navigation. In 51.4% of the patients, the puncture was performed by the landmark method. The overall success rate was 100% (n = 52) in the real-time ultrasound navigation group, 96.4% (n = 53) in the landmark insertion group, (p = 0.496). The first percutaneous puncture success rate was 57.7% (n = 30) in the real-time ultrasound navigation group and 45.5% (n = 25) in the landmark insertion group, (p = 0.460). The data show a higher overall success rate and the first success rate in the US-guided CVC insertion group, but the difference was not statistically significant.
A Multicenter Cohort Study Evaluating the Teratogenic Effects of Isotretinoin on Neonates
Authors: Brzezinski Piotr , Zonda Gabriela Ildiko , Hincu Maura Adelina , Vasilache Ingrid-Andrada , Chiriac Anca , Ciuhodaru Madalina Irina , Borowska Katarzyna , Paduraru Luminita
Published: October 2022

(1) Background: Isotretinoin (ISO) is a systemic retinoid known for its teratogenic effects on embryos and fetuses. The aim of this study was to compare the pregnancy outcomes of women who were exposed to isotretinoin with those of women without such exposure from a teratogenic point of view. (2) Methods: A total of 1459 female patients from three clinical hospitals in Poland and Romania, segregated into two groups depending on their ISO exposure, were evaluated between January and December 2019. Medical records were screened to identify the pregnancy outcomes and congenital malformation rates. (3) Results: The congenital malformation rate for the exposed group was 1.2% (four cases), and no specific signs of Accutane embryopathy were identified. Women from the unexposed group were more likely to deliver preterm and through cesarean deliveries and had a higher rate of newborn congenital malformations as compared to women from the exposed group. (4) Conclusions: Even though we could not find a significant association between ISO exposure and teratogenic effects in newborns, effective contraceptive measures are key to preventing unfavorable pregnancy outcomes.
Apoptotic MSCs and MSC-Derived Apoptotic Bodies as New Therapeutic Tools
Authors: Kholodenko Irina V. , Kholodenko Roman V. , Majouga Alexander G. , Yarygin Konstantin N.
Published: October 2022

Over the past two decades, mesenchymal stem cells (MSCs) have shown promising therapeutic effects both in preclinical studies (in animal models of a wide range of diseases) and in clinical trials. However, the efficacy of MSC-based therapy is not always predictable. Moreover, despite the large number of studies, the mechanisms underlying the regenerative potential of MSCs are not fully elucidated. Recently, it has been reliably established that transplanted MSCs can undergo rapid apoptosis and clearance from the recipient’s body, still exhibiting therapeutic effects, especially those associated with their immunosuppressive/immunomodulating properties. The mechanisms underlying these effects can be mediated by the efferocytosis of apoptotic MSCs by host phagocytic cells. In this concise review, we briefly describe three types of MSC-generated extracellular vesicles, through which their therapeutic functions can potentially be carried out; we focused on reviewing recent data on apoptotic MSCs and MSC-derived apoptotic bodies (MSC-ApoBDs), their functions, and the mechanisms of their therapeutic effects.
A Review of Effects of Femtosecond Laser Parameters on Metal Surface Properties
Authors: Sun Hongfei , Li Jiuxiao , Liu Mingliang , Yang Dongye , Li Fangjie
Published: October 2022

As a laser technology, the femtosecond laser is used in biomedical fields due to its excellent performance—its ultrashort pulses, high instantaneous power, and high precision. As a surface treatment process, the femtosecond laser can prepare different shapes on metal surfaces to enhance the material’s properties, such as its wear resistance, wetting, biocompatibility, etc. Laser-induced periodic surface structures (LIPSSs) are a common phenomenon that can be observed on almost any material after irradiation by a linearly polarized laser. In this paper, the current research state of LIPSSs in the field of biomedicine is reviewed. The influence of laser parameters (such as laser energy, pulse number, polarization state, and pulse duration) on the generation of LIPSSs is discussed. In this paper, the applications of LIPSSs by femtosecond laser modification for various purposes, such as in functional surfaces, the control of surface wettability, the surface colonization of cells, and the improvement of tribological properties of surfaces, are reviewed.
Mitigating CMAS Attack in Model YAlO3 Environmental Barrier Coatings: Effect of YAlO3 Crystal Orientation on Apatite Nucleation
Authors: Velázquez Plaza Amanda , Krause Amanda R.
Published: October 2022

Environmental barrier coatings (EBCs) are used to protect ceramic-matrix composites from undesirable reactions with steam and calcia–magnesia–alumina–silicate (CMAS) particulates found in gas-turbine engine environments. Effective EBCs contain yttria or rare earth ions that will react with molten CMAS to form a protective apatite layer that prevents further attack. Methods to improve the EBCs’ CMAS mitigation capabilities focus on improving the apatite yield but neglect optimizing the apatite formation behavior. This study investigates the effect of apatite nucleation behavior on CMAS penetration by comparing the CMAS attack at 1350 °C of four different single crystal orientations of yttria aluminate perovskite (YAP), a promising EBC candidate. The EBC/CMAS interfacial energy and, thus, reaction behavior varies with YAP orientation. In regions with low CMAS loading, rapid apatite growth is seen on YAP substrates with orientations associated with high EBC/CMAS interfacial energy. However, CMAS penetration is most significant in these samples because the apatite growth is facilitated by recession of the YAP substrate nearby. Such behavior is not observed in regions with high CMAS loading where small apatite crystals form on top of an yttrium aluminate garnet (Y3Al5O12, YAG) phase. This study shows that strategies that control the nucleation and growth of apatite will provide better protection against CMAS.
Marine Biofilm Model Comprising a Loop-Type Biofilm Reactor and a Halomonas Strain HIG FST4 1, an Active Biofilm-Forming Bacterium
Authors: Ogawa Akiko , Hosaka Shoya , Kanematsu Hideyuki , Yoshitake Michiko
Published: October 2022

In ocean and coastal waters, the formation of biofilms on artificial matters often causes intractable phenomena such as the deterioration of surface functions and corrosion, resulting in significant economic damage. Thus, methods for inhibiting biofilm formation are in high demand, and many new anti-biofilm products are being designed on a daily basis. However, practical and safe assays for evaluating anti-biofilm formation have not yet been established. In this study, we developed a more practical and safer biofilm formation test system composed of a loop-type laboratory biofilm reactor (LBR) and HIG FST4 1, a Halomonas strain derived from ballast seawater, in comparison with a slowly rotating test-tube culture (TTC) test. To evaluate biofilm formation in an LBR and TTC, three materials (pure iron, pure aluminum, and soda lime glass) were tested, and Raman spectroscopic analysis was used for the identification and relative quantification of the biofilm contents. Regardless of the test method, biofilm formation progressed in the order of soda lime glass < pure aluminum < pure iron. The Raman peaks showed that the LBR test samples tended to remove polysaccharides compared to the TTC test samples and that the proportion of proteins and lipids in the LBR test samples was much higher than that of the TTC test samples. These results show that the combination of HIG FST4 1 and LBR is suitable for biofilm formation in a practical marine environment.
Nonlinear Numerical Analysis and Restoring force Model of Composite Joints with Steel Reinforced Recycled Concrete Columns and Steel Beams
Authors: Dong Jing , Shen Changling , Ma Hui , Liu Yunhe , Cong Xiaoran
Published: October 2022

Cyclic loading tests were conducted for eight composite joints (five interior joints and three exterior joints) with steel reinforced recycled concrete (SRRC) columns and steel beams under cyclic loading. The axial compression ratio and recycled coarse aggregate (RCA) replacement percentage were considered as the main design parameters for the above composite joints. The seismic behavior of the composite frame joints with (SRRC) columns and steel beams were studied by numerical simulation and the constitutive models of recycled aggregate concrete (RAC), steel and reinforcement were given to the corresponding elements. The experimental results were used to validate the finite element (FE) model. The results indicated that the FE model can accurately predict the deformation and stress load-strain curves of the composite joints. Then, a FE parameter analysis was carried out on the seismic behavior of the composite joints. The RAC strength, steel strength and column steel web thickness were considered as the main parameters. Results pinpointed that the increase in RAC strength and steel web thickness of the columns significantly affected the horizontal bearing capacity of the composite joints. However, with the increase in RAC and steel strength, the deformation ability and ductility of composite joints were almost unaffected. The reasonable increase in column steel web thickness can improve the seismic ductility of composite joints, evidently. On this basis, considering the stress characteristics of joint specimens, a four broken line restoring force model for steel reinforced recycled concrete column steel beam composite frame joints was established. This model includes the skeleton curve model, the stiffness degradation pattern and hysteresis rules. The calculation curve was in good agreement with experimental curves, which can well reflect the hysteretic behavior of joints subjected to low cyclic repeated loading. The above results can be used as a reference for the seismic design of composite frame joints.
CMOS-Compatible Optoelectronic Imagers
Authors: Bi Cheng , Liu Yanfei
Published: October 2022

Silicon-based complementary metal oxide semiconductors have revolutionized the field of imaging, especially infrared imaging. Infrared focal plane array imagers are widely applied to night vision, haze imaging, food selection, semiconductor detection, and atmospheric pollutant detection. Over the past several decades, the CMOS integrated circuits modified by traditional bulk semiconductor materials as sensitivity sensors for optoelectronic imagers have been used for infrared imaging. However, traditional bulk semiconductor material-based infrared imagers are synthesized by complicated molecular beam epitaxy, and they are generally coupled with expensive flip-chip-integrated circuits. Hence, high costs and complicated fabrication processes limit the development and popularization of infrared imagers. Emerging materials, such as inorganic–organic metal halide perovskites, organic polymers, and colloidal quantum dots, have become the current focus point for preparing CMOS-compatible optoelectronic imagers, as they can effectively decrease costs. However, these emerging materials also have some problems in coupling with readout integrated circuits and uniformity, which can influence the quality of imagers. The method regarding coupling processes may become a key point for future research directions. In the current review, recent research progress on emerging materials for infrared imagers is summarized.
Solidification Microstructure Prediction of Ti-6Al-4V Alloy Produced by Laser Melting Deposition
Authors: Liu Jin , Lv Hang , Xie Shao , Han Ruipeng , Zhang Zhenlin , Liu Yan , Chen Hui , Chen Yong , She Jian , He Dupeng
Published: October 2022

The ability to achieve a predictable solidification microstructure would greatly accelerate the qualification of the additive manufacturing process. Solidification microstructure control is a challenging issue for the additive manufacturing of metallic components using the laser melting deposition (LMD) method. To obtain desirable microstructure characteristics and mechanical properties, it is essential to research the solidification mechanism of microstructures initiated during the LMD process. In this study, the grain morphology and size of an LMD-fabricated Ti-6Al-4V alloy were predicted using a three-dimensional cellular automaton (CA) model coupled with a finite element (FE) model (CA–FE). First, the temperature distribution and solidification microstructure were established with the multi-scale CA–FE model, and the simulated results were shown to be in qualitative agreement with the experimental results. Moreover, the effects of the process parameters on both the thermal characteristics and the solidification microstructure were identified, and the morphologies and sizes of prior β grains under different laser power levels and scanning speeds were compared. The average grain size of the molten pool was shown to decrease with decreasing incident energy (lower laser power/higher scanning speed), and columnar-to-equiaxed transformation could be achieved under the proper processing parameters. This work will serve as a guide for the optimization and regulation of microstructures in the LMD process.
Study on Damage Behavior of NiCoCrAlY/MSZ Plasma-Sprayed Coating in Neutral Salt Spray Environment
Authors: Li Zhongsheng , Wu Hulin , Bai Yixin , Cong Dalong , Huang Anwei , Song Kaiqiang , Zhang Min , Wei Zixiang , Ding Xingxing , Wang Xuan , Peng Dong
Published: October 2022

In order to explore the corrosion damage behavior of plasma-sprayed NiCoCrAlY/magnesia-stabilized zirconia (18~22 wt.% MgO-ZrO2, MSZ) thermal barrier and anti-ablation coatings in a salt spray environment, a series of neutral salt spray (NSS) tests for 96 h (N-2), 192 h (N-4) and 288 h (N-6) were carried out on the coatings. The microstructures, composition distributions, potentiodynamic polarization curves, bonding strengths and anti-ablation properties of the coatings before and after the NSS tests were characterized. The results show that, with the increase in salt spray time, the expansion and overflow of corrosion products (FexOy) led to more defects forming inside the coating; the porosity of the top coat (TC) of N-6 increased to 15.2% in comparison to that of the blank control sample (N-0), which was 8.5%. According to the potentiodynamic polarization curves, accelerated corrosion occurred in the coating samples after the salt spray time reached 192 h, and the corrosion current density rapidly surged from 2.2 × 10−8 A·cm−2 (N-4) to 2.2 × 10−5 A·cm−2 (N-6). Similar degradation tends could be found in the bonding strengths and mass ablation rates of the coatings due to the accumulation of loose corrosion products on the substrate, which affected the bonding states of the coatings and promoted their spalling under an oxyacetylene flame (OAF). As a result, the maximum service life of the NiCoCrAlY/MSZ coatings in the neutral salt spray environment was about 192 h. This work is instructive for studying the environmental adaptability of metal/ceramic double-layer coatings.
Nickel-Assisted Transfer-Free Technology of Graphene Chemical Vapor Deposition on GaN for Improving the Electrical Performance of Light-Emitting Diodes
Authors: Tang Penghao , Xiong Fangzhu , Du Zaifa , Li Kai , Mei Yu , Guo Weiling , Sun Jie
Published: October 2022

With the rapid development of graphene technology, today graphene performs well in the application of light-emitting diode (LED) transparent electrodes. Naturally, high-quality contact between the graphene and the GaN underneath is very important. This paper reports a process for nickel-assisted transfer-free technology of graphene chemical vapor deposition on GaN. The nickel film plays the dual role of etching mask and growth catalyst, and is removed by the subsequent “penetration etching” process, achieving good direct contact between the graphene and GaN. The results show that the graphene effectively improves the current spreading of GaN-based LEDs and enhances their electrical performance. This scheme avoids the wrinkles and cracks of graphene from the transfer process, and is not only suitable for the combination of graphene and GaN-based LEDs, but also provides a solution for the integration of graphene and other materials.
Estimation of the Critical Value of the Second-Phase Particles in the Microstructure of AZ31 Mg Alloy by Phase-Field Methods
Authors: Wu Yan , Xiong Jinlin , Luo Qiang , Chen Jibing , Zeng Rutie , Wang Shuo
Published: October 2022

In this study, phase-field models were employed to simulate the effects of second-phase particles (SPPs) on grain growth of the AZ31 Mg alloy, under realistic spatial and temporal scales, at 350 °C, during annealing. The particle sizes ranged from 0 to 7 μm, and the particles with large volume fractions were used in the paper. The results reveal that the volume fractions and sizes of the SPP affect grain growth and that the volume fractions and sizes of the SPP on pinning exhibited critical values. When the SPP volume fraction is f = 5%, the SPP is at the maximum critical size, rμmmax; when the SPP size is r=1 μm, the SPP minimum critical volume fraction is fmin = 0.25% and the maximum critical volume fraction is fmax = 20%. The critical values increase with the increase of the sizes or volume fractions of the second-phase particles. Finally, the average grain size, particle size, and particle volume fraction obtained from the simulation were fitted according to the Zener relationship, and the obtained results showed that the fitting indices were in the range of 0.33–0.50. The results were compared with the experimental results. The simulation results obtained in this study will provide an important academic reference for understanding the mechanism and law of grain growth, an important reference for accurate control of grain size and properties of the material, a reference for the development of the annealing treatment process of Mg alloy, and a theoretical guide for the use of recrystallization process to control the microstructure of Mg alloy and improve the plastic-forming properties.
Platelet-to-White Blood Cell Ratio: A Feasible Biomarker for Pyogenic Liver Abscess
Authors: Ko Dong-Gyun , Park Ji-Won , Kim Jung-Hee , Jung Jang-Han , Kim Hyoung-Su , Suk Ki-Tae , Jang Myoung-Kuk , Park Sang-Hoon , Lee Myung-Seok , Kim Dong-Joon , Kim Sung-Eun
Published: October 2022

The platelet-to-white blood cell ratio (PWR) has been reported to predict the severity of patients with various diseases. However, no previous studies have assessed the use of the PWR as a prognostic marker for pyogenic liver abscesses (PLA). This observational retrospective study was performed between January 2008 and December 2017, including 833 patients with PLA from multiple centers. The enrolled patients, on average, had a PWR of 17.05, and 416 patients had a PWR lower than 17.05. A total of 260 patients (31.2%) with PLA showed complications of metastatic infection, pleural effusion and abscess rupture. A low PWR level was identified as a strong risk factor for metastatic infection and pleural effusion. The low PWR group also had a longer hospital stay. In the multivariate analysis, old age, anemia, albumin and CRP levels and unidentified pathogens were significant factors for low PWR levels. A low PWR, old age, male sex, abscess size, albumin, ALP and unidentified causative pathogens showed significant associations with a hospital stay longer than 28 days. As a result, PLA patients presenting with a low PWR were shown to have more complications and a poor prognosis. Considering its cost-effectiveness, PWR could be a novel biomarker used to predict a prognosis of PLA.
Multiregional Radiomic Signatures Based on Functional Parametric Maps from DCE-MRI for Preoperative Identification of Estrogen Receptor and Progesterone Receptor Status in Breast Cancer
Authors: Zhong Shiling , Wang Fan , Wang Zhiying , Zhou Minghui , Li Chunli , Yin Jiandong
Published: October 2022

Radiomics based on dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) has been used for breast estrogen receptor (ER) and progesterone receptor (PR) status evaluation. However, the radiomic features of peritumoral regions were not thoroughly analyzed. This study aimed to establish and validate the multiregional radiomic signatures (RSs) for the preoperative identification of the ER and PR status in breast cancer. A total of 443 patients with breast cancer were divided into training (n = 356) and validation (n = 87) sets. Radiomic features were extracted from intra- and peritumoral regions on six functional parametric maps from DCE-MRI. A two-sample t-test, least absolute shrinkage and selection operator regression, and stepwise were used for feature selections. Three RSs for predicting the ER and PR status were constructed using a logistic regression model based on selected intratumoral, peritumoral, and combined intra- and peritumoral radiomic features. The area under the receiver operator characteristic curve (AUC) was used to assess the discriminative performance of three RSs. The AUCs of intra- and peritumoral RSs for identifying the ER status were 0.828/0.791 and 0.755/0.733 in the training and validation sets, respectively. For predicting the PR status, intra- and peritumoral RSs resulted in AUCs of 0.816/0.749 and 0.806/0.708 in the training and validation sets, respectively. Multiregional RSs achieved the best AUCs among three RSs for evaluating the ER (0.851 and 0.833) and PR (0.848 and 0.763) status. In conclusion, multiregional RSs based on functional parametric maps from DCE-MRI showed promising results for preoperatively evaluating the ER and PR status in breast cancer patients. Further studies using a larger cohort from multiple centers are necessary to confirm the reliability of the established models before clinical application.
Improving the Detection Sensitivity of a New Rapid Diagnostic Technology for Severe Acute Respiratory Syndrome Coronavirus 2 Using a Trace Amount of Saliva
Authors: Tokuyama-Toda Reiko , Terada-Ito Chika , Muraoka Masaaki , Horiuchi Toshikatsu , Amemiya Tsuyoshi , Fukuoka Airi , Hamada Yoshiki , Takebe Yusuke , Ogawa Takashi , Fujii Seiko , Kikuta Toshihiro , Sejima Shunsuke , Satomura Kazuhito
Published: October 2022

The early diagnosis and isolation of infected individuals with coronavirus disease 2019 (COVID-19) remain important. Although quantitative polymerase chain reaction (qPCR) testing is considered the most accurate test available for COVID-19 diagnosis, it has some limitations, such as the need for specialized laboratory technicians and a long turnaround time. Therefore, we have established and reported a rapid diagnostic method using a small amount of saliva as a sample using a lightweight mobile qPCR device. This study aimed to improve the existing method and increase the detection sensitivity and specificity. The detection specificity of CDC N1 and N2 was examined by improving qPCR reagents and polymerase chain reaction conditions for the previously reported method. Furthermore, the feasibility of detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral RNA was examined using both the previous method and the improved method in patients with COVID-19. The results showed that the improved method increased the specificity and sensitivity. This improved method is useful for the rapid diagnosis of SARS-CoV-2.
Serum and CSF Biomarkers Predict Active Early Cognitive Decline Rather Than Established Cognitive Impairment at the Moment of RRMS Diagnosis
Authors: Tiu Vlad Eugen , Popescu Bogdan Ovidiu , Enache Iulian Ion , Tiu Cristina , Terecoasa Elena , Panea Cristina Aura
Published: October 2022

(1) Background: Cognitive impairment (CI) begins early in the evolution of multiple sclerosis (MS) but may only become obvious in the later stages of the disease. Little data is available regarding predictive biomarkers for early, active cognitive decline in relapse remitting MS (RRMS) patients. (2) Methods: 50 RRMS patients in the first 6 months following diagnosis were included. The minimum follow-up was one year. Biomarker samples were collected at baseline, 3-, 6- and 12-month follow-up. Cognitive performance was assessed at baseline and 12-month follow-up; (3) Results: Statistically significant differences were found for patients undergoing active cognitive decline for sNfL z-scores at baseline and 3 months, CSF NfL baseline values, CSF Aβ42 and the Bremso score as well. The logistic regression model based on these 5 variables was statistically significant, χ2(4) = 22.335, p < 0.0001, R2 = 0.671, with a sensitivity of 57.1%, specificity of 97.4%, a positive predictive value of 80% and a negative predictive value of 92.6%. (4) Conclusions: Our study shows that serum biomarkers (adjusted sNfL z-scores at baseline and 3 months) and CSF biomarkers (CSF NfL baseline values, CSF Aβ42), combined with a clinical score (BREMSO), can accurately predict an early cognitive decline for RRMS patients at the moment of diagnosis.