bluuebunny/crossref_metadata_2025 huggingface dataset:
Hygroscopic larval provisions of bees absorb soil water vapor and release liquefied nutrients
Authors: Cane James H. , Love Byron G.
Published: October 2021

Larvae of most bee species consume individual provision masses composed of pollen mixed with nectar. For simple metabolic reasons, mature larvae should weigh less than their consumed provision. However, past research reported a remarkable result: mature larvae of three ground-nesting halictid bees weighed 60% more than their original provision masses. This surprising paradox could result from the expected hygroscopic nature of nectar. Sugar solutions absorb water vapor at rates defined by their osmolarity and ambient humidity. Our experiments tested this hypothesis, showing that larval provisions of a ground-nesting bee, Nomia melanderi, are strongly hygroscopic. They consequently absorbed substantial water vapor from this bee’s preferred nesting soil. Mature larvae weighed 65% more than their original provision because hygroscopy had greatly augmented available dietary water. Liquid accumulating around isolated provisions was a sweet nutritious broth that included amino acids leached from the pollen. Hygroscopy was most intense during the egg and early larval stages. However, provision liquefaction (and possible drowning) was partly offset by rapid hydration of cached pollen, whose weight could double after absorbing free water. Larval provisions of two cavity-nesting Osmia species also readily absorbed water vapor from a soil atmosphere. However, at humidities measured within tunnels of their natural deadwood nesting substrates, they gained little weight via hygroscopy. Consequently, their mature larvae weighed less, not more, than the provision that they ate. These new insights explain some nesting traits shared by many ground-nesting bees, such as why females do not waterproof the earthen cell caps of their nest cells, or why many colletids cache liquid provisions. Progressive hygroscopy and resulting sugar dilution may also mediate succession of microbial mutualists and pathogens in provision masses of ground-nesting bees.
Concept development and testing of the UK’s first hydrogen-hybrid train (HydroFLEX)
Authors: Calvert Charles , Allan Jeff , Amor Peter , Hillmansen Stuart , Roberts Clive , Weston Paul
Published: October 2021

In October 2018, Porterbrook and the University of Birmingham announced the HydroFLEX project, to demonstrate a hydrogen-hybrid modified train at Rail Live 2019. The concept of modifying a Class 319 Electric Multiple Unit was developed, with equipment including a fuel cell stack, traction battery, 24 V control system and hydrogen storage elements to be mounted inside one of the carriages. This was followed by procurement of a fuel cell stack, traction batteries, and control equipment, which was then installed inside the train, being fixed to the seat rails. One substantial change from the concept was the provision of considerably more hydrogen storage than the minimum necessary, providing the train with more potential to be further modified to allow for higher speed mainline testing. After the Rail Live exhibition where HydroFLEX was demonstrated, numerous modifications were performed to increase the reliability and power of the HydroFLEX train, primarily concerned with modifying the base train logic, with the aim of a successful mainline test. Supporting this effort was a multitude of documentation concerning safety, operations, and approvals to gain approvals from the relevant approvals bodies. The project demonstrated the feasibility of using hydrogen fuel cells as an autonomous fuel for railway propulsion systems, which has the potential for full decarbonisation.
A review of hydrogen technologies and engineering solutions for railway vehicle design and operations
Authors: Sun Yan , Anwar M. , Hassan N. M. S. , Spiryagin Maksym , Cole Colin
Published: October 2021

Interest in hydrogen-powered rail vehicles has gradually increased worldwide over recent decades due to the global pressure on reduction in greenhouse gas emissions, technology availability, and multiple options of power supply. In the past, research and development have been primarily focusing on light rail and regional trains, but the interest in hydrogen-powered freight and heavy haul trains is also growing. The review shows that some technical feasibility has been demonstrated from the research and experiments on proof-of-concept designs. Several rail vehicles powered by hydrogen either are currently operating or are the subject of experimental programmes. The paper identifies that fuel cell technology is well developed and has obvious application in providing electrical traction power, while hydrogen combustion in traditional IC engines and gas turbines is not yet well developed. The need for on-board energy storage is discussed along with the benefits of energy management and control systems.
Adult postgraduate students’ learning in online spaces—Images of identity and belonging
Authors: Dennis Carol Azumah , Clifton Gill
Published: October 2021

This research seeks to animate the voices of postgraduate students registered on a UK distance learning online Masters in Education or Childhood & Youth programme. Such a critical exploration is timely given the HE landscape is premised on its openness and accessibility. Our study reports on 33 interviews with postgraduate students using photo elicitation and unstructured interviews. We prioritise the perspectives of students whose experiences do not replicate the success stories which generally epitomize representations of HE study, favouring instead the voices of students who interrupted or in some cases terminated their studies. Our aim is to better understand the PG students’ personal, professional, and academic learning trajectories.

In reading the data we produced four “manifesto” statements crafted from a series of dialogues between ourselves as researchers, our colleagues, the online experiences of adult postgraduate students and our reading of literatures surrounding withdrawal, persistence and retention. Interpretations gravitated towards four themes: identity, belonging, digital pedagogies and uncanny spaces and which point towards students’ perspectives about the interconnections between identity and belonging and how these concepts help develop understanding of “social presence”, what Bayne (2008) and Cartens (2016) assert as “uncanny” spaces. Our manifesto statements represent our reading of the data to stimulate further thinking around the HE digital pedagogy landscapes. The four statements have implications for how we understand, participate in and manage postgraduate adult students’ learning in digital spaces.
Formation of Humboldtine During the Dissolution of Hematite in Oxalic Acid – Density Functional Theory (DFT) Calculations and Experimental Verification
Authors: Vehmaanperä Paula , Gong Bo , Sit Patrick H.-L. , Salmimies Riina , Barbiellini Bernardo , Häkkinen Antti
Published: October 2021

Understanding the reactions taking place in the hematite-oxalic acid system is important in order to clean iron oxides from filters and to remove iron from mineral concentrates. Previous studies reported the formation of an unwanted solid phase during this process. The objective of the current work, therefore, was to visualize and rationalize the iron dissolution steps taking place in the hematite–oxalic acid reaction by combining density functional theory (DFT) calculations and experimental data. The results of DFT calculations indicated that a precipitate was formed in this reaction; XRD analysis of the solid phase after the dissolution experiment revealed the formation of humboldtine as the precipitate. The attachment of oxalate on the hematite surface and the reduction of Fe(III) to Fe(II) were key steps for humboldtine formation. Both simulations and the experimental results showed that greater oxalic acid concentrations yielded more precipitate, suggesting a simple and novel route to synthesize humboldtine, a material which is relevant to the demand for clean energy.
Single-Cell Transcriptomes Reveal Characteristics of MicroRNAs in Gene Expression Noise Reduction
Authors: Hu Tao , Wei Lei , Li Shuailin , Cheng Tianrun , Zhang Xuegong , Wang Xiaowo
Published: October 2021

Isogenic cells growing in identical environments show cell-to-cell variations because of the stochasticity in gene expression. High levels of variation or noise can disrupt robust gene expression and result in tremendous consequences for cell behaviors. In this work, we showed evidence from single-cell RNA sequencing data analysis that microRNAs (miRNAs) can reduce gene expression noise at the mRNA level in mouse cells. We identified that the miRNA expression level, number of targets, target pool abundance, and miRNA–target interaction strength are the key features contributing to noise repression. miRNAs tend to work together in cooperative subnetworks to repress target noise synergistically in a cell type-specific manner. By building a physical model of post-transcriptional regulation and observing in synthetic gene circuits, we demonstrated that accelerated degradation with elevated transcriptional activation of the miRNA target provides resistance to extrinsic fluctuations. Together, through the integrated analysis of single-cell RNA and miRNA expression profiles, we demonstrated that miRNAs are important post-transcriptional regulators for reducing gene expression noise and conferring robustness to biological processes.
The NGO Moment
Authors: O'Sullivan Kevin
Published: October 2021

This book is a study of compassion as a global project from Biafra to Live Aid. Kevin O'Sullivan explains how and why NGOs became the primary conduits of popular concern for the global poor between the late 1960s and the mid-1980s and shows how this shaped the West's relationship with the post-colonial world. Drawing on case studies from Britain, Canada and Ireland, as well as archival material from governments and international organisations, he sheds new light on how the legacies of empire were re-packaged and re-purposed for the post-colonial era, and how a liberal definition of benevolence, rooted in charity, justice, development and rights became the dominant expression of solidarity with the Third World. In doing so, the book provides a unique insight into the social, cultural and ideological foundations of global civil society. It reveals why this period provided such fertile ground for the emergence of NGOs and offers a fresh interpretation of how individuals in the West encountered the outside world.
The Rights and Obligations of States in Disputed Maritime Areas
Authors: van Logchem Youri
Published: October 2021

Disputed maritime areas exist around the world, and in many cases the affected States have not been able to reach agreement on cooperative arrangements or regulate commercial activities within the area. Where a claimant coastal State increases their level of unilateral activity, such as in the South China Sea, long-standing conflicts remain difficult to resolve. This book examines the rights and obligations States have under international law concerning disputed maritime areas in the first comprehensive treatment of this highly topical and pressing issue. It analyses conventional law, general international law, judicial decisions, State practice and academic opinions that shine light on the international legal framework applicable in disputed maritime areas. Proposing practical solutions for interpreting and applying the applicable international law, the book discusses the extent to which it currently provides clear guidance to States, and how international courts and tribunals have dealt with cases related to disputed maritime areas.
What Makes a Good Judge? Perspectives from Indonesia
Authors: Butt Simon
Published: October 2021

In May 2018, Artidjo Alkostar retired from the Supreme Court of Indonesia after a judicial career spanning almost two decades. Over this period, he presided over many of Indonesia’s most prominent and controversial criminal cases and became renowned for routinely rejecting corruption appeals and increasing prison sentences. In the celebratory publications that marked his retirement, Alkostar was held up as a model judge, with senior legal figures, including Supreme Court judges, singling out his strong work ethic, integrity, simplicity of character, and firmness. Curiously absent from the list of praiseworthy attributes were pre-requisites for effective judging, including adequate legal knowledge, transparent legal reasoning and decision-making, objectivity and avoiding the perception of bias. An analysis of Alkostar’s most notorious decisions suggests that he, and the judges who served with him, did not always clearly display these pre-requisites. This article considers what this says about judging in Indonesia and what might, in practice, be the defining characteristics of a good judge there.
A Quantitative Analysis of Legislation with Harsher Punishment in Japan
Authors: Kyo Shunsuke
Published: October 2021

The purpose of this study is to show how the Japanese government has created laws with harsher punishment since the 1990s. While a tendency toward harsher punishment is common in advanced Western countries, a similar tendency in Japan has prompted scholarly discussion on whether it can be understood through the “penal-populism” framework. However, it lacks in systematic evidence. This study presents three findings that differ from previous studies through a quantitative analysis of legislation with harsher punishment. First, while previous literature argues that the legislation increased in the latter half of the 1990s, this study shows that it peaked in the middle of the 2000s. Second, while previous literature argues that the bureaucrats of the Ministry of Justice promote the legislation, this study shows that it is caused by every ministry’s drafting Bills. Third, this study shows that it does not quantitatively avoid partisan conflicts, contrary to the prediction of the “penal-populism” theory.
Instrumental support exchanges among kin and non-kin in light of personal configurations
Authors: Aeby Gaëlle , Gauthier Jacques-Antoine
Published: October 2021

As exchanges of instrumental support between kin and non-kin remain essential to buffer the impact of critical life events, we consider the characteristics of personal configurations that may enhance or hinder them. Personal configurations vary in terms of their composition and two aspects of their structure: density and centrality. These dimensions are investigated to uncover whether they influence the type of instrumental support being exchanged (financial, material, and care) and the likelihood of their being reciprocal. Drawing on a representative sample of individuals living in Switzerland, results show that overall instrumental support is present in all personal configurations irrespective of their composition, but financial support is more prevalent in configurations based on parent–adult child relationships. Furthermore, configuration structures characterized by density of emotional support are positively associated with giving care support and with reciprocity, while those characterized by density of conflict are associated with giving less instrumental support overall.
Drones, Afghanistan, and beyond: Towards analysis and assessment in context
Authors: Page James Michael , Williams John
Published: October 2021

Afghanistan is one of the most drone-affected states, however, very few scholarly studies of drone use there exist. This article uses original fieldwork data in a strategically important area of Afghanistan, eastern Nangarhar province, to analyse drone effects in context. It raises important concerns about the ability of the US to attain stated counterterrorism and counterinsurgency goals. Our results show contextualised analysis of drone use necessitates appropriate enquiry into policy, doctrine, and local circumstances. It also reveals serious incoherence, inaccurate assumptions, and insufficient appreciation of local circumstances and dynamics not only in US policy and doctrine, but also numerous scholarly studies of drone use effectiveness. This has far-reaching policy, operational, and research implications, including regarding local communities’ governance, relations, and resilience to insurgent and terrorist encroachment. Therefore, we contribute to debates about how to analyse and assess drone use and its effects, why drone analysis needs to change, and what more effective forms of research and analysis can reveal.
2-kW-level superfluorescent fiber source with flexible wavelength and linewidth tunable characteristics
Authors: Ye Jun , Fan Chenchen , Xu Jiangming , Xiao Hu , Leng Jinyong , Zhou Pu
Published: October 2021

The superfluorescent fiber source (SFS) with tunable optical spectrum has shown great application potential in the sensing, imaging, and spectral combination. Here, we demonstrate for the first time a 2-kW-level wavelength and linewidth tunable SFS. Based on a flexible filtered SFS seed and three stages of fiber amplifiers, the output power can be scaled from the milliwatt level to about 2 kW, with a wavelength tuning range of 1068–1092 nm and a linewidth tuning range of 2.5–9.7 nm. Moreover, a numerical simulation is conducted based on the generalized nonlinear Schrödinger equation, and the results reveal that the wavelength tuning range is limited by the decrease of seed power and the growth of amplified spontaneous emission, whereas the linewidth tuning range is determined by the gain competition and nonlinear Kerr effects. The developed wavelength and linewidth tunable SFS may be applied to scientific research and industrial processing.
Low-order modelling of the wake dynamics of an Ahmed body
Authors: Podvin Bérengère , Pellerin Stéphanie , Fraigneau Yann , Bonnavion Guillaume , Cadot Olivier
Published: October 2021

We investigate the large-scale signature of the random switches between two mirrored turbulent wake states of flat-backed bodies. A direct numerical simulation (DNS) of the flow around an Ahmed body at a Reynolds number ($Re$) of 10 000 is considered. Using proper orthogonal decomposition (POD), we identify the most energetic modes of the velocity field and build a low-dimensional model based on the first six fluctuating velocity modes capturing the characteristics of the flow dynamics during and between switches. In the absence of noise, the model produces random switches with characteristic time scales in agreement with the simulation and experiments. This chaotic model suggests that random switches are triggered by the increase of the vortex shedding activity. However, the addition of noise results in a better agreement in the temporal spectra of the coefficients between the model and the simulation.
Association of adherence to the Australian Dietary Guidelines with cognitive performance and cognitive decline in the Sydney Memory and Ageing Study: a longitudinal analysis
Authors: Chen Xi , Liu Zhixin , Sachdev Perminder S. , Kochan Nicole A. , Brodaty Henry , O'Leary Fiona
Published: October 2021

This study investigated associations of adherence to the Australian Dietary Guidelines (ADG) with cognitive performance and cognitive decline over 6 years. We used longitudinal data from the Sydney Memory and Aging Study comprising 1037 community-dwelling non-demented participants aged 70–90 years. Dietary intake was assessed at baseline using the Dietary Questionnaire for Epidemiological Studies Version 2. Adherence to the ADG was scored using the Dietary Guideline Index 2013 (DGI-2013). Cognition was assessed using neuropsychological tests in six cognitive domains and global cognition at baseline and 2, 4 and 6 years later. Linear mixed models analysed the association between adherence to the ADG and cognitive function and cognitive decline over 6 years. Results indicated that overall adherence to the ADG was suboptimal (DGI-2013 mean score 43⋅8 with a standard deviation of 10⋅1; median score 44, range 12–73 with an interquartile range of 7). The percent of participants attaining recommended serves for the five food groups were 30⋅2 % for fruits, 11⋅2 % for vegetables, 54⋅6 % for cereals, 28⋅9 % for meat and alternatives and 2⋅1 % for dairy consumption. Adherence to the ADG was not associated with overall global cognition over 6 years (β = 0⋅000; 95 % CI: −0⋅007, 0⋅007; P = 0⋅95). Neither were DGI-2013 scores associated with change in global cognitive performance over 6 years (β = 0⋅002; 95 % CI: −0⋅002, 0⋅005; P = 0⋅41) nor in any individual cognitive domains. In conclusion, adherence to the ADG was not associated with cognitive health over time in this longitudinal analysis of older Australians. Future research is needed to provide evidence to support specific dietary guidelines for neurocognitive health among Australian older adults.
Patents, healthcare and engaged shareholders: a pathway to encourage socially responsible patent use?
Authors: McMahon Aisling , Richardson Edana
Published: October 2021

This paper focuses on the drivers steering companies’ behaviour over uses of patented health technologies, taking Covid-19 as a case study. Global equitable access to health technologies is vital to bringing the pandemic under control. Reflecting this, global mechanisms for rightsholders to share intellectual property rights, data and know-how over such health technologies have been developed. Yet, to date, there is limited support from corporate rightsholders for such mechanisms. Instead, health technologies have been licensed largely based on bilateral deals, with vast global inequalities emerging. Given the traditional focus within company law on prioritising shareholders' short-term financial value, we argue that it is unsurprising that many corporate rightsholders adopt a protectionist approach to patents, even in the face of health crises. However, we argue that the tide may now be starting to shift, catalysed by an emergence of engaged shareholders petitioning for socially responsible corporate behaviour, including for uses of intellectual property over health technologies in a manner that more clearly aligns with public interests. If harnessed and encouraged, such engaged shareholder behaviour could present an opportunity to reframe the conception of shareholder value towards one that considers a long-term sustainable approach and ultimately to shift corporate behaviour around uses of intellectual property over health technologies to take public interests into account.
DO L1-L2 DIFFERENCES IN DISCOURSE PROCESSING REFLECT PROCESSING DEMANDS OR DIFFICULTY OF FORM-FUNCTION MAPPING?
Authors: Lee Eun-Kyung , Fraundorf Scott
Published: October 2021

We examined what causes L1-L2 differences in sensitivity to prominence cues in discourse processing. Participants listened to recorded stories in segment-by-segment fashion at their own pace. Each story established a pair of contrasting items, and one item from the pair was rementioned and manipulated to carry either a contrastive or presentational pitch accent. By directly comparing the current self-paced listening data to previously obtained experimenter-paced listening data, we tested whether reducing online-processing demands allows L2 learners to show a nativelike behavior, such that contrastive pitch accents facilitate later ruling out the salient alternative. However, reduced time pressure failed to lead even higher proficiency L1-Korean learners of English to reach a nativelike level, suggesting that L2 learners’ nonnativelike processing and representation of the prominence cue in spoken discourse processing can be due to the inherent difficulty of fully learning a complex form-function mapping rather than to online-processing demands.
Analysis of outcomes in patients with abnormal laterality undergoing congenital heart surgery
Authors: Basgoze Serdar , Temur Bahar , Guvenc Osman , Aydin Selim , Guzelmeric Fusun , Onalan Mehmet A. , Erek Ersin
Published: October 2021

Objective:

Laterality anomalies are almost always associated with severe cardiac anomalies. Demographic properties, type of the procedures, associated anomalies, and early and mid-term prognosis of four types of laterality anomalies were analysed.

Methods:

A total of 64 consecutive patients with laterality anomalies were enrolled between July 2014 and July 2020. We grouped the patients as situs solitus dextrocardia (SSD) (n = 12; 18.7%); situs inversus (SI) (n = 16; 25%); right atrial isomerism (RAI) (n = 29; 45.3%); and left atrial isomerism (LAI) (n = 7; 10.9%). TAPVC was only present in the RAI group (31%). Incidence of mitral or tricuspid atresia was higher in the SSD group (25%). All the patients were followed up with a mean of 19.06 ± 17.6 (0.1–72) months.

Results:

Early postoperative mortality was 17 patients, among 107 procedures (15.8%). Twelve patients were in the neonatal period. All ten patients survived after isolated ductal stenting. Fourteen of the deaths were in the RAI group (48.3%). The 3-year survival rates were 85% in LAI, 78.7% in SI, 55.8% in SSD, and 38% in RAI groups. According to the multivariable Cox regression model, mechanical ventilation, kidney injury, RAI, and complex surgery in the neonatal period were independent risk factors for early mortality.

Conclusion:

Laterality anomalies are one of the most challenging patients who commonly had univentricular physiology. The most prevalent anomaly was RAI, and RAI had the worst outcome and survival. Ductal stent is an acceptable first intervention during the neonatal period in suitable patients. Complex procedures may carry a high risk of death in the neonatal period.
Pharmacologic predictors of benzodiazepine response trajectory in anxiety disorders: a Bayesian hierarchical modeling meta-analysis
Authors: Stimpfl Julia N. , Mills Jeffrey A. , Strawn Jeffrey R.
Published: October 2021

Background

Despite frequent benzodiazepine use in anxiety disorders, the trajectory and magnitude of benzodiazepine response and the effects of benzodiazepine potency, lipophilicity, and dose on improvement are unknown.

Methods

We performed a meta-analysis using weekly symptom severity data from randomized, parallel group, placebo-controlled trials of benzodiazepines in adults with anxiety disorders. Response was modeled for the standardized change in continuous measures of anxiety using a Bayesian hierarchical model. Change in anxiety was evaluated as a function of medication, disorder, time, potency, lipophilicity, and standardized dose and compared among benzodiazepines.

Results

Data from 65 trials (73 arms, 7 medications, 7110 patients) were included. In the logarithmic model of response, treatment effects emerged within 1 week of beginning treatment (standardized benzodiazepine-placebo difference = −0.235 ± 0.024, CrI: −0.283 to −0.186, P < .001) and placebo response plateaued at week 4. Doses <6 mg per day (lorazepam equivalents) produced faster and larger improvement than higher doses (P = .039 for low vs medium dose and P = .005 for high vs medium dose) and less lipophilic benzodiazepines (beta = 0.028 ± 0.013, P = .030) produced a greater response over time. Relative to the reference benzodiazepine (lorazepam), clonazepam (beta = −0.217 ± 0.95, P = .021) had a greater trajectory/magnitude of response (other specific benzodiazepines did not statistically differ from lorazepam).

Conclusions

In adults with anxiety disorders, benzodiazepine-related improvement emerges early, and the trajectory and magnitude of improvement is related to dose and lipophilicity. Lower doses and less lipophilic benzodiazepines produce greater improvement.
Perceived neighbourhood food access is associated with consumption of animal-flesh food, fruits and vegetables among mothers and young children in peri-urban Cambodia
Authors: Duong Minh-Cam , Nguyen-Viet Hung , Grace Delia , Ty Chhay , Sokchea Huy , Sina Vor , Young Melissa F
Published: October 2021

Objective:

To examine whether mothers’ perceived neighbourhood food access is associated with their own and their young children’s consumption of animal-flesh food, fruits and vegetables in peri-urban areas of Cambodia.

Design:

A cross-sectional survey measured food consumption frequency and perceived neighbourhood food access, the latter including six dimensions of food availability, affordability, convenience, quality, safety and desirability. Multivariate logistic regression was used to assess the association between perceived food access and food consumption.

Setting:

Peri-urban districts of Phnom Penh and Siem Reap, Cambodia

Participants:

198 mothers of children between 6 and 24 months old.

Results:

Over 25 % of the mothers and 40 % of the children had low consumption (< once a day) of either animal-flesh food or fruits and vegetables. Compared with perceived high food access, perceived low food access was associated with an adjusted 5·6-fold and 4·3-fold greater odds of low animal-flesh food consumption among mothers (95 % CI 2·54, 12·46) and children (95 % CI 2·20, 8·60), respectively. Similarly, relative to perceived high food access, perceived low food access was associated with 7·6-times and 5·1-times higher adjusted odds of low fruits and vegetables consumption among mothers (95 % CI 3·22, 18·02) and children (95 % CI 2·69, 9·83), respectively.

Conclusions:

Mothers’ perceived neighbourhood food access was an important predictor of their own and their young children’s nutrient-rich food consumption in peri-urban Cambodia. Future work is needed to confirm our findings in other urban settings and examine the role of neighbourhood food environment in the consumption of both nutrient-rich and nutrient-poor food.
Improving the Quantification of Deuterium in Zirconium Alloy Atom Probe Tomography Data Using Existing Analysis Methods
Authors: Jones Megan E , London Andrew J , Breen Andrew J , Styman Paul D , Sikotra Shyam , Moody Michael P , Haley Daniel
Published: October 2021

Zirconium alloys are common fuel claddings in nuclear fission reactors and are susceptible to the effects of hydrogen embrittlement. There is a need to be able to detect and image hydrogen at the atomic scale to gain the experimental evidence necessary to fully understand hydrogen embrittlement. Through the use of deuterium tracers, atom probe tomography (APT) is able to detect and spatially locate hydrogen at the atomic scale. Previous works have highlighted issues with quantifying deuterium concentrations using APT due to complex peak overlaps in the mass-to-charge-state ratio spectrum between molecular hydrogen and deuterium (H2 and D). In this work, we use new methods to analyze historic and simulated atom probe data, by applying currently available data analysis tools, to optimize solving peak overlaps to improve the quantification of deuterium. This method has been applied to literature data to quantify the deuterium concentrations in a concentration line profile across an α-Zr/deuteride interface.
Appraisal of the conversion possibilities of pastoral meat sheep systems to the organic production model
Authors: Ruiz Francisco de Asís , Grande Daniel , Nahed José , Castel José María , Mena Yolanda
Published: October 2021

In the Mediterranean Basin, sheep meat production systems are based on grazing, and help to conserve biodiversity, mitigate the greenhouse gases emissions and maintain the population in rural areas. However, the lack of differentiation of pastoral systems, as opposed to the intensive model, puts its continuity at risk. In this sense, organic farming can be an alternative to sustain extensive sheep-cereal production systems in marginal Mediterranean drylands. The aim of this research was to evaluate the conversion possibilities of pastoral meat sheep production systems to the organic production model in the Mediterranean Basin, working with the autochthonous Segureña breed sheep. 46 farms were studied, classified into four clusters by mean of multivariate analysis. In order to determine the degree to which farms approached the organic model, 60 variables, grouped into nine indicators were analyzed to obtain an Organic Conversion Index (OCI). The Nutritional management indicator has a high value of approaching the organic model, as well as Animal welfare and Food safety. Marketing and business management, Breeds and reproduction, Weed and pest control and Sustainable pasture management indicators also reach a good approximation level. Only the Breeds and reproduction indicator presented values with significant differences between clusters. The average value of the OCI for the 46 farms ranges from 63 to 70%, and therefore it can be concluded that extensive meat sheep herds in the region are close to this production model. Among the recommendations that can be made to improve the conversion possibilities to the organic model are: (i) to increase own fodder production or find a way to obtain it easily and economically; (ii) to complete the plant−soil−animal cycle, (iii) to seek greater marketing autonomy and (iv) to achieve closer contact with the final consumer. At the same time, government policy both in Spain and other parts of Europe should persevere to find more ways to support the progress of this type of production, in an effort to address limitations and overcome the lack of alternative markets.
Linear electromagnetic inverse scattering via generative adversarial networks
Authors: Zhou Huilin , Zheng Huimin , Liu Qiegen , Liu Jian , Wang Yuhao
Published: October 2021

Electromagnetic inverse-scattering problems (ISPs) are concerned with determining the properties of an unknown object using measured scattered fields. ISPs are often highly nonlinear, causing the problem to be very difficult to address. In addition, the reconstruction images of different optimization methods are distorted which leads to inaccurate reconstruction results. To alleviate these issues, we propose a new linear model solution of generative adversarial network-based (LM-GAN) inspired by generative adversarial networks (GAN). Two sub-networks are trained alternately in the adversarial framework. A linear deep iterative network as a generative network captures the spatial distribution of the data, and a discriminative network estimates the probability of a sample from the training data. Numerical results validate that LM-GAN has admirable fidelity and accuracy when reconstructing complex scatterers.
pyUserCalc: A Revised Jupyter Notebook Calculator for Uranium‐Series Disequilibria in Basalts
Authors: Elkins Lynne J. , Spiegelman Marc
Published: October 2021

Key Points

Cloud‐based Jupyter notebook presents an open source, reproducible tool for modeling U‐series in basalts

Equilibrium and pure disequilibrium porous flow U‐series models with 1D conservation of mass

Scaled porous flow model introduces incomplete equilibrium scenario with reaction rate limitations
Plastic Deformation of Plagioclase in Oceanic Gabbro Accreted at a Slow‐Spreading Ridge (Hole U1473A, Atlantis Bank, Southwest Indian Ridge)
Authors: Allard Maël , Ildefonse Benoît , Oliot Émilien , Barou Fabrice
Published: October 2021

Crustal architecture at slow‐spreading oceanic ridges results from complex interactions between magmatism, hydrothermalism, and tectonics. IODP Hole U1473A was drilled during Expeditions 360 and 362T at the summit of the Atlantis Bank, a gabbroic massif exhumed at the Southwest Indian Ridge. In this study, we identify and quantify plastic deformation processes in gabbroic lithologies and active slip systems in plagioclase from 115 microstructural domains throughout Hole U1473A. We describe deformed zones using petrographic observations and electron backscattered diffraction analyses made all along the core. Ductile deformation is widespread, and in places strongly localized in mylonitic and ultramylonitic zones. Plagioclase represents ∼60% of rock's volume and is the dominant phase accommodating deformation in samples. It shows strong dynamic recrystallization accommodated by subgrain rotation in the dislocation creep regime, forming a fine‐grained matrix. Electron backscattered diffraction analyses reveal weak to moderate crystallographic preferred orientations of plagioclase as a result of plastic deformation and strain localization, producing a fabric characterized by (010) parallel to the foliation plane and [100] parallel to the lineation. The fabric strength is first increasing from slightly deformed lithologies to mylonites before decreasing significantly in ultramylonites. This could be explained by orientation scattering after recrystallization, and a change of active slip systems. Subsequent granular flow has likely occurred in some samples. A detailed investigation of intracrystalline misorientations measured at plagioclase subgrain boundaries reveals the activity of four dominant slip systems: [001](010), [100](001), ½[110](001), and ½[](001). These slip systems reflect decreasing temperatures during CPO development and subgrain wall formation.
Alzheimer’s disease profiled by fluid and imaging markers: tau PET best predicts cognitive decline
Authors: Bucci Marco , Chiotis Konstantinos , Nordberg Agneta ,   for the Alzheimer’s Disease Neuroimaging Initiative
Published: October 2021

For early detection of Alzheimer’s disease, it is important to find biomarkers with predictive value for disease progression and clinical manifestations, such as cognitive decline. Individuals can now be profiled based on their biomarker status for Aβ42 (A) or tau (T) deposition and neurodegeneration (N). The aim of this study was to compare the cerebrospinal fluid (CSF) and imaging (PET/MR) biomarkers in each ATN category and to assess their ability to predict longitudinal cognitive decline. A subset of 282 patients, who had had at the same time PET investigations with amyloid-β and tau tracers, CSF sampling, and structural MRI (18% within 13 months), was selected from the ADNI dataset. The participants were grouped by clinical diagnosis at that time: cognitively normal, subjective memory concern, early or late mild cognitive impairment, or AD. Agreement between CSF (amyloid-β-1-42(A), phosphorylated-Tau181(T), total-Tau(N)), and imaging (amyloid-β PET (florbetaben and florbetapir)(A), tau PET (flortaucipir)(T), hippocampal volume (MRI)(N)) positivity in ATN was assessed with Cohen’s Kappa. Linear mixed-effects models were used to predict decline in the episodic memory. There was moderate agreement between PET and CSF for A biomarkers (Kappa = 0.39–0.71), while only fair agreement for T biomarkers (Kappa ≤ 0.40, except AD) and discordance for N biomarkers across all groups (Kappa ≤ 0.14) was found. Baseline PET tau predicted longitudinal decline in episodic memory irrespective of CSF p-Tau181 positivity (p ≤ 0.02). Baseline PET tau and amyloid-β predicted decline in episodic memory (p ≤ 0.0001), but isolated PET amyloid-β did not. Isolated PET Tau positivity was only observed in 2 participants (0.71% of the sample). While results for amyloid-β were similar using CSF or imaging, CSF and imaging results for tau and neurodegeneration were not interchangeable. PET tau positivity was superior to CSF p-Tau181 and PET amyloid-β in predicting cognitive decline in the AD continuum within 3 years of follow-up.
Epithelial argininosuccinate synthetase is dispensable for intestinal regeneration and tumorigenesis
Authors: van der Meer Jonathan H. M. , de Boer Ruben J. , Meijer Bartolomeus J. , Smit Wouter L. , Vermeulen Jacqueline L. M. , Meisner Sander , van Roest Manon , Koelink Pim J. , Dekker Evelien , Hakvoort Theodorus B. M. , Koster Jan , Hawinkels Lukas J. A. C. , Heijmans Jarom , Struijs Eduard A. , Boermeester Marja A. , van den Brink Gijs R. , Muncan Vanesa
Published: October 2021

The epithelial signaling pathways involved in damage and regeneration, and neoplastic transformation are known to be similar. We noted upregulation of argininosuccinate synthetase (ASS1) in hyperproliferative intestinal epithelium. Since ASS1 leads to de novo synthesis of arginine, an important amino acid for the growth of intestinal epithelial cells, its upregulation can contribute to epithelial proliferation necessary to be sustained during oncogenic transformation and regeneration. Here we investigated the function of ASS1 in the gut epithelium during tissue regeneration and tumorigenesis, using intestinal epithelial conditionalAss1knockout mice and organoids, and tissue specimens from colorectal cancer patients. We demonstrate that ASS1 is strongly expressed in the regenerating andApc-mutated intestinal epithelium. Furthermore, we observe an arrest in amino acid flux of the urea cycle, which leads to an accumulation of intracellular arginine. However, loss of epithelialAss1does not lead to a reduction in proliferation or increase in apoptosis in vivo, also in mice fed an arginine-free diet. Epithelial loss ofAss1seems to be compensated by altered arginine metabolism in other cell types and the liver.
BRCA1 prevents R-loop-associated centromeric instability
Authors: Racca Carine , Britton Sébastien , Hédouin Sabrine , Francastel Claire , Calsou Patrick , Larminat Florence
Published: October 2021

Centromeres are defined by chromatin containing the histone H3 variant CENP-A assembled onto repetitive α-satellite sequences, which are actively transcribed throughout the cell cycle. Centromeres play an essential role in chromosome inheritance and genome stability through coordinating kinetochores assembly during mitosis. Structural and functional alterations of the centromeres cause aneuploidy and chromosome aberrations which can induce cell death. In human cells, the tumor suppressor BRCA1 associates with centromeric chromatin in the absence of exogenous damage. While we previously reported that BRCA1 contributes to proper centromere homeostasis, the mechanism underlying its centromeric function and recruitment was not fully understood. Here, we show that BRCA1 association with centromeric chromatin depends on the presence of R-loops, which are non-canonical three-stranded structures harboring a DNA:RNA hybrid and are frequently formed during transcription. Subsequently, BRCA1 counteracts the accumulation of R-loops at centromeric α-satellite repeats. Strikingly, BRCA1-deficient cells show impaired localization of CENP-A, higher transcription of centromeric RNA, increased breakage at centromeres and formation of acentric micronuclei, all these features being R-loop-dependent. Finally, BRCA1 depletion reveals a Rad52-dependent hyper-recombination process between centromeric satellite repeats, associated with centromere instability and missegregation. Altogether, our findings provide molecular insights into the key function of BRCA1 in maintaining centromere stability and identity.
Long-term safety and efficacy of intramyocardial adenovirus-mediated VEGF-DΔNΔC gene therapy eight-year follow-up of phase I KAT301 study
Authors: Leikas Aleksi J. , Hassinen Iiro , Hedman Antti , Kivelä Antti , Ylä-Herttuala Seppo , Hartikainen Juha E. K.
Published: October 2021

In phase I KAT301 trial, intramyocardial adenovirus-mediated vascular endothelial growth factor -DΔNΔC (AdVEGF-D) gene therapy (GT) resulted in a significant improvement in myocardial perfusion reserve and relieved symptoms in refractory angina patients at 1-year follow-up without major safety concerns. We investigated the long-term safety and efficacy of AdVEGF-D GT. 30 patients (24 in VEGF-D group and 6 blinded, randomized controls) were followed for 8.2 years (range 6.3–10.4 years). Patients were interviewed for the current severity of symptoms (Canadian Cardiovascular Society class, CCS) and perceived benefit from GT. Medical records were reviewed to assess the incidence of major cardiovascular adverse event (MACE) and other predefined safety endpoints. MACE occurred in 15 patients in VEGF-D group and in five patients in control group (21.5 vs. 24.9 per 100 patient-years; hazard ratio 0.97; 95% confidence interval 0.36–2.63; P = 0.95). Mortality and new-onset comorbidity were similar between the groups. Angina symptoms (CCS) were less severe compared to baseline in VEGF-D group (1.9 vs. 2.9; P = 0.006) but not in control group (2.2 vs. 2.6; P = 0.414). Our study indicates that intramyocardial AdVEGF-D GT is safe in the long-term. In addition, the relief of symptoms remained significant during the follow-up.
Geographical drivers and climate-linked dynamics of Lassa fever in Nigeria
Authors: Redding David W. , Gibb Rory , Dan-Nwafor Chioma C. , Ilori Elsie A. , Yashe Rimamdeyati Usman , Oladele Saliu H. , Amedu Michael O. , Iniobong Akanimo , Attfield Lauren A. , Donnelly Christl A. , Abubakar Ibrahim , Jones Kate E. , Ihekweazu Chikwe
Published: October 2021

Lassa fever is a longstanding public health concern in West Africa. Recent molecular studies have confirmed the fundamental role of the rodent host (Mastomys natalensis) in driving human infections, but control and prevention efforts remain hampered by a limited baseline understanding of the disease’s true incidence, geographical distribution and underlying drivers. Here, we show that Lassa fever occurrence and incidence is influenced by climate, poverty, agriculture and urbanisation factors. However, heterogeneous reporting processes and diagnostic laboratory access also appear to be important drivers of the patchy distribution of observed disease incidence. Using spatiotemporal predictive models we show that including climatic variability added retrospective predictive value over a baseline model (11% decrease in out-of-sample predictive error). However, predictions for 2020 show that a climate-driven model performs similarly overall to the baseline model. Overall, with ongoing improvements in surveillance there may be potential for forecasting Lassa fever incidence to inform health planning.
Single-molecule junction spontaneously restored by DNA zipper
Authors: Harashima Takanori , Fujii Shintaro , Jono Yuki , Terakawa Tsuyoshi , Kurita Noriyuki , Kaneko Satoshi , Kiguchi Manabu , Nishino Tomoaki
Published: October 2021

The electrical properties of DNA have been extensively investigated within the field of molecular electronics. Previous studies on this topic primarily focused on the transport phenomena in the static structure at thermodynamic equilibria. Consequently, the properties of higher-order structures of DNA and their structural changes associated with the design of single-molecule electronic devices have not been fully studied so far. This stems from the limitation that only extremely short DNA is available for electrical measurements, since the single-molecule conductance decreases sharply with the increase in the molecular length. Here, we report a DNA zipper configuration to form a single-molecule junction. The duplex is accommodated in a nanogap between metal electrodes in a configuration where the duplex is perpendicular to the nanogap axis. Electrical measurements reveal that the single-molecule junction of the 90-mer DNA zipper exhibits high conductance due to the delocalized π system. Moreover, we find an attractive self-restoring capability that the single-molecule junction can be repeatedly formed without full structural breakdown even after electrical failure. The DNA zipping strategy presented here provides a basis for novel designs of single-molecule junctions.
Circadian neurons in the paraventricular nucleus entrain and sustain daily rhythms in glucocorticoids
Authors: Jones Jeff R. , Chaturvedi Sneha , Granados-Fuentes Daniel , Herzog Erik D.
Published: October 2021

Signals from the central circadian pacemaker, the suprachiasmatic nucleus (SCN), must be decoded to generate daily rhythms in hormone release. Here, we hypothesized that the SCN entrains rhythms in the paraventricular nucleus (PVN) to time the daily release of corticosterone. In vivo recording revealed a critical circuit from SCN vasoactive intestinal peptide (SCNVIP)-producing neurons to PVN corticotropin-releasing hormone (PVNCRH)-producing neurons. PVNCRH neurons peak in clock gene expression around midday and in calcium activity about three hours later. Loss of the clock gene Bmal1 in CRH neurons results in arrhythmic PVNCRH calcium activity and dramatically reduces the amplitude and precision of daily corticosterone release. SCNVIP activation reduces (and inactivation increases) corticosterone release and PVNCRH calcium activity, and daily SCNVIP activation entrains PVN clock gene rhythms by inhibiting PVNCRH neurons. We conclude that daily corticosterone release depends on coordinated clock gene and neuronal activity rhythms in both SCNVIP and PVNCRH neurons.