bluuebunny/crossref_metadata_2025 huggingface dataset:
Delineation of structure elements and the basement depth at the Jifara plain NW LIBYA using integration application of potential field dataset
Authors: Trepil Fouzie
Published: February 2021

In this study, the analysis of ground gravity data (from the Libyan Petroleum Institute) and aeromagnetic data (from the African Magnetic Mapping Project) are used to delineate the structure elements and basement depth within the Jifara plain, northwest Libya. A high-pass filter and a reduction-to-the-pole (RTP) transformation are applied to the gravity and aeromagnetic data respectively. The Total horizontal gradient and Centre for Exploration Targeting (CET) grid analysis are applied to gravity datasets. Werner deconvolution is applied to the RTP magnetic data. One 2-D forward modelling profile is applied to gravity and magnetic dataset. The results show gravity anomaly highs and magnetic anomaly with different structure trends. Werner deconvolution profile shows a high-density intrusion (about 1.8 km from the surface) within the sedimentary sequence. The intrusion may be the result of the Eocene volcanic activities near the Gharyan region. The 2-D model shows that the basement depth of Jifara plain ranges from 2.8 to 3.0 km. The integration results of the gravity and magnetic dataset reflect various periods of tectonic activities within Jifara plain and the adjacent area.
On disappointment: promoting ordinary conversations in extraordinary times
Authors: Scanlon Christopher
Published: February 2021

To be disappointed is to be human, to be disappointing is also to be human. This article will invite reflection upon the under-theorised phenomenon of disappointment and its relationship to ‘failure’, to ‘hope’ and perhaps even ‘forgiveness’ (or the lack if it). The central premise is that to engage with ‘disappointment’ in our internal relatedness, and in our interpersonal and social relationships may enable us to re-connect with our own and others’ humanity – and not to do so is to remain stuck, aggrieved, resentful and locked into cycles of reciprocal self- and other-destructive violence and recrimination. The article will seek to explore disappointment as a ‘disturbance of groupishness’ (Bion, 1961, emphasis added), ‘a location  of  disturbance’ (Foulkes, 1948/1983 emphasis added) and a way of structuring the traumatised organisation-in-the-mind (Armstrong, 2005; Scanlon, 2012). The article will conclude with an invitation for psycho-social practitioners to leave our psycho-social retreats (consulting rooms, libraries, classrooms and the like) and, once again, to engage more deliberatively with conversations in ‘public spheres’ (Habermas, 1968).
The role of parental and child physical and mental health on behavioural and emotional adjustment in mid-childhood: a comparison of two generations of British children born 30 years apart
Authors: Parsons Sam , Sullivan Alice , Fitzsimons Emla , Ploubidis George
Published: February 2021

Poor physical health and behavioural and emotional problems in childhood have a lasting impact on well-being in adolescence and adulthood. Here we address the relationship between poor parent and child physical and mental health in early childhood (age 5) and conduct, hyperactivity and emotional problems in mid-childhood (age 10/11). We compare results across two generations of British children born 30 years apart in 1970 (n = 15,856) and 2000/2 (16,628). We take advantage of rich longitudinal birth cohort data and establish that a child’s own poor health was associated with conduct, hyperactivity and emotional problems in mid-childhood in both generations, and that with the exception of conduct problems in the 1970 cohort these relationships remained when family socio-economic status and individual characteristics were accounted for. Poor maternal mental health was similarly associated with conduct, hyperactivity and emotional problems in both generations; poor parental physical health with a child having later hyperactivity and emotional problems in the younger generation. Results also indicated that earlier behaviour problems had more influence on later problems for children in the more recent cohort. Given the increasing proportion of children and adolescents with mental health problems and that socio-economic disadvantage increases physical and mental well-being concerns within families, policy solutions must consider the holistic nature of a child’s family environment to prevent some children experiencing a ‘double whammy’ of disadvantage. The early years provide the best opportunity to promote children’s resilience and well-being and minimise the development of entrenched negative behaviours and their subsequent costs to society.
Neoliberalism, austerity and social work with children and families: challenges and critical/radical possibilities
Authors: Rogowski Steve
Published: February 2021

In the UK, neoliberalism and associated austerity have dominated social work and welfare provision over the last decade. Consequences include severe financial cuts to social work with children and families, as well as public services generally, and large increases in poverty and inequality. Despite increasing numbers of people in difficulty, the social work and welfare system has become more punitive and presents ongoing threats to social work’s commitment to human rights and social justice. This article examines such developments and includes the views of practitioners. Despite the strength and depth of challenges, it argues that critical/radical possibilities remain for practitioners to work both individually with service users and collectively. Such opportunities need to be taken with a view to working towards a more just and equal society, this being a much-needed antidote to the unequal neoliberal world we currently inhabit.
‘You can’t repeal regret’: targeting men for mobilisation in Ireland’s abortion debate
Authors: Hunt Kate , Friesen Amanda
Published: February 2021

This study explores how social movement organisations involved in the abortion debate in the Republic of Ireland attempted to appeal to men in their campaign messages before the 2018 referendum on the Eighth Amendment concerning abortion. We scrape social movement organisations’ Twitter accounts to conduct quantitative and qualitative content analyses of images and videos the organisations posted, and find evidence that social movement organisations sometimes extended their frames to men as voters. Social movement organisations evoked themes of hegemonic masculinity in their imagery and messaging, though these themes were not a large portion of overall campaign tweets and there were distinct differences in how this was done by the two organisations we study. Previous research suggests anti-abortion organisations extend their frames to incorporate ‘pro-woman’ messaging. Our research contributes by exploring the ways that frames may be extended by both anti- and pro-abortion actors to target men and mobilise masculinity in public debates over women’s rights.
Introduction: Support workers and the health professions
Authors: Saks Mike
Published: February 2021

The introduction highlights the growing global importance of support workers and the need for further social scientific analysis of their developing role in relation to health professions, especially in neo-liberal societies. In so doing, it provides a brief overview of the constituent chapters of the book and how they hang together, including the neo-Weberian theoretical frame of reference. This overview underlines the need for further policy consideration internationally in this area, encompassing both the public and private sector as support workers are increasingly involved in health care, in association with professionalised groups such as doctors, midwives and nurses. In this light, the introduction asks what specific regulatory policy reforms might occur to provide a higher quality support worker labour force that will enhance the protection of clients and the public and foster positive work conditions in Western societies – based on an appropriate skill mix and interface with the health professions. In so doing, it builds on the empirical research on which this collection is based within the neo-Weberian theoretical framework that binds this book together.
Health professionals and peer support workers in mental health settings
Authors: Leemeijer Aukje , Noordegraaf Mirko
Published: February 2021

In many Western countries mental health care institutions employ ‘peer support workers’ in professional teams. They are clients or former clients in mental health care who are trained and educated to transform their personal experience as a client into ‘experiential knowledge’ helping other clients. This is supposed to improve ‘client centeredness’ in mental health care. However, the rise and roles of peer support workers are not undisputed; mental health professionals – psychiatrists, psychologists, nurses and therapists – have ambivalent responses. Peer support workers may challenge the status and dominance of traditional expert knowledge when they bring experiential knowledge into decision making processes. This challenge is strengthened by the development of peer support workers as a new group who may ultimately themselves professionalise. This chapter, focused on The Netherlands, explores from a neo-Weberian standpoint the relationship between mental health professionals and peer support workers. This is related to professional identities and positions, and also institutional surroundings, including how peer support workers and service organisations deal with risks and accountability. On the basis of empirical observational research, organisational conditions are shown to count more than occupational conditions. This underscores that the interweaving of new forms of knowledge in service processes must be organised.
Noninvasive and real-time monitoring of Au nanoparticle promoted cancer metastasis using in vivo flow cytometry
Authors: Pang Wen , Ding Shihui , Lin Liyun , Wang Chen , Lei Man , Xu Jiale , Wang Xintong , Qu Junle , Wei Xunbin , Gu Bobo
Published: February 2021

Cancer is the second leading cause of mortality globally, while cancer metastasis, which accounts for about 90% of cancer-related mortality, presents an extremely poor prognosis. Thus, various nanomedicines were designed and synthesized for cancer treatment, but nanomaterials could lead to endothelial leakiness and consequently facilitate intravasation and extravasation of cancer cells to form circulating tumor cells (CTCs), which were regarded as the potential metastatic seeds, possibly accelerating cancer metastasis. Neither possible metastatic sites were observed nor rare CTCs could be measured using common methods at the early stage of cancer metastasis, it is urgent to explore new technology to dynamically monitor nanomedicine promoted cancer metastasis with high sensitivity, which would be beneficial for cancer treatment as well as design and synthesis of nanomedicine. Herein, a novel optical biopsy tool i.e. in vivo flow cytometry (IVFC) was constructed to noninvasively and real-time monitor CTCs of tumor-bearing mice treated with various concentrations of Au nanoparticles. The in vivo experimental results demonstrated the promoted CTCs were Au nanoparticles dose-dependent consistent with the in vitro results, which showed Au nanoparticles induced dose-dependent gaps in the blood vessel endothelial walls to accelerate CTCs formation, making IVFC a promising biopsy tool in fundamental, pre-clinical and clinical investigation of nanomedicine and cancer metastasis.
A 1.4 kW 780 nm pulsed diode laser, high duty cycle, passively side-cooled pump module
Authors: Hübner M. , Wilkens M. , Eppich B. , Maaßdorf A. , Martin D. , Ginolas A. , Basler P. S. , Crump P.
Published: February 2021

A diode laser module emitting 1.4 kW optical in-pulse power near 780 nm optimized for high (≥ 10%) duty-cycle operation in a micro-channel free design is presented. With full collimation, a beam quality with a nearly symmetric M2 of 205 × 295 (vertical × horizontal direction) for a wide range of pulse widths is found.
Tunable wave plates based on phase-change metasurfaces
Authors: Zhang Dong-Qin , Shu Fang-Zhou , Jiao Zhi-Wei , Wu Hong-Wei
Published: February 2021

Wave plates based on metasurfaces have attracted intensive attention over the past decade owing to their compactness and design flexibility. Although various wave plates have been designed, their working wavelengths are fixed once they are made. Here we present a study on tunable wave plates based on phase-change metasurfaces made of Ge2Sb2Te5 nanopillar structures. The Ge2Sb2Te5 nanopillars can work as a high-efficiency transmissive half- or quarter-wave plate depending on their structural parameters. The working wavelength of wave plate can be tuned via the phase transition of Ge2Sb2Te5. Moreover, the polarization state of the transmitted light at a fixed wavelength can be modified by changing the crystallinity of Ge2Sb2Te5. The features suggest that tunable wave plates may have applications in optical modulators, molecular detection, and polarimetric imaging.
Excitation of high-quality orbital angular momentum vortex beams in an adiabatically helical-twisted single-mode fiber
Authors: Ren Kaili , Ren Liyong , Liang Jian , Yang Li , Xu Jie , Han Dongdong , Wang Yongkai , Liu Jihong , Dong Jun , He Hanyu , Zhang Wenfei
Published: February 2021

A novel method to control the parameters of a chiral fiber grating structure is proposed. Mode couplings are controlled in real time during the twisting fabrication process. This chiral grating structure can satisfy the phase-matching condition for generating high-quality orbital angular momentum (OAM) beams, with an order mode of conversion efficiency over 99.9%. Both theoretical analysis and experimental results of this OAM mode conversion have been investigated, with good agreement. The results demonstrate a dual-OAM beam converter with a charge of ±1 for the right- and left-handed CLPGs, respectively. The high-quality OAM beam generated in this twisted single-mode fiber process may find excellent applications in optical communications.
Outcomes from international field trials with Male Aedes Sound Traps: Frequency-dependent effectiveness in capturing target species in relation to bycatch abundance
Authors: Staunton Kyran M. , Leiva Donovan , Cruz Alvaro , Goi Joelyn , Arisqueta Carlos , Liu Jianyi , Desnoyer Mark , Howell Paul , Espinosa Francia , Mendoza Azael Che , Karl Stephan , Crawford Jacob E. , Xiang Wei , Manrique-Saide Pablo , Achee Nicole L. , Grieco John P. , Ritchie Scott A. , Burkot Thomas R. , Snoad Nigel
Published: February 2021

Aedes aegypti and Aedes albopictus vector dengue, chikungunya and Zika viruses. With both species expanding their global distributions at alarming rates, developing effective surveillance equipment is a continuing priority for public health researchers. Sound traps have been shown, in limited testing, to be highly species-specific when emitting a frequency corresponding to a female mosquito wingbeat. Determining male mosquito capture rates in sound traps based on lure frequencies in endemic settings is the next step for informed deployment of these surveillance tools. We field-evaluated Male Aedes Sound Traps (MASTs) set to either 450 Hz, 500 Hz, 550 Hz or 600 Hz for sampling Aedes aegypti and/or Aedes albopictus and compared catch rates to BG-Sentinel traps within Pacific (Madang, Papua New Guinea) and Latin American (Molas, Mexico and Orange Walk Town, Belize) locations. MASTs set to 450–550 Hz consistently caught male Ae. aegypti at rates comparable to BG-Sentinel traps in all locations. A peak in male Ae. albopictus captures in MASTs set at 550 Hz was observed, with the lowest mean abundance recorded in MASTs set to 450 Hz. While significantly higher abundances of male Culex were sampled in MASTs emitting lower relative frequencies in Molas, overall male Culex were captured in significantly lower abundances in the MASTs, relative to BG-Sentinel traps within all locations. Finally, significant differences in rates at which male Aedes and Culex were positively detected in trap-types per weekly collections were broadly consistent with trends in abundance data per trap-type. MASTs at 550 Hz effectively captured both male Ae. aegypti and Ae. albopictus while greatly reducing bycatch, especially male Culex, in locations where dengue transmission has occurred. This high species-specificity of the MAST not only reduces staff-time required to sort samples, but can also be exploited to develop an accurate smart-trap system—both outcomes potentially reducing public health program expenses.
Establishing and evaluation of a polymerase chain reaction for the detection of Echinococcus multilocularis in human tissue
Authors: Grimm Johannes , Krickl Julian , Beck Annika , Nell Juliane , Bergmann Monika , Tappe Dennis , Grüner Beate , Barth Thomas FE , Brehm Klaus
Published: February 2021

Background

Alveolar echinococcosis (AE) is caused by metacestode larva of the tapeworm Echinococcus multilocularis. AE diagnostics currently rely on imaging techniques supported by serology, but unequivocal detection of AE is difficult. Although polymerase chain reaction (PCR)-based methods to detect tapeworm DNA in biopsies have been suggested for several species, no validated protocol adhering to accepted guidelines has so far been presented for AE diagnostics. We herein established a PCR protocol for metacestode biopsies and technically evaluated the method using isolated parasite DNA and cells, biopsies of clinically relevant material, and formalin fixed paraffin-embedded (FFPE) human tissue blocks. We compared the results with an immunochemical (IHC) approach using the monoclonal antibody Em2G11 specific for the antigen Em2 of E. mulitlocularis.

Methodology/Principal findings

Based on tapeworm 12S rDNA sequences we established and validated a PCR protocol for robust detection of as little as 50 parasite cells per specimen and report 127 cases of positive identification of Echinococcus species in samples from humans and animals. For further validation, we analyzed 45 liver, heart, brain, and soft tissue samples as well as cytological probes of aspirates of FFPE-material from 18 patients with clinically confirmed AE. Of each patient we analyzed (i) fully viable lesions with laminated layer; (ii) tissue with mAbEm2G11-positive small particles of E. multilocularis (spems); (iii) mAbEm2G11-negative tissue adjacent to the main lesion; and (iv) lymph node tissue with mAbEm2G11-positive spems. To identify the areas for the PCR-based approach, we performed IHC-staining with the monoclonal antibody Em2G11. Micro-dissected tissue of these areas was then used for PCR-analysis. 9 of 15 analyzed samples with viable E. multilocularis lesions with laminated layer were positive by PCR. Of this group, all samples preserved for less than 6 years (6/6) were tested positive. 11 of 15 samples of spems and 7 of 9 samples of the control group mAbEm2G11-negative tissue were negative by PCR. We further show that all probes from lymph nodes with spems are PCR negative.

Conclusions/Significance

We present a sensitive PCR method for the detection of E. multilocularis in human tissue, particularly in fresh biopsy material and tissue blocks stored for less than 5 years. While the diagnostic sensitivity of material containing only spems was higher using IHC, PCR detection was possible in IHC negative liver tissue and in patients with negative serology. Our results support the view that spems do not contain parasitic DNA or viable cells of the parasite. spems thus most probably do not directly contribute to metastasis formation during AE.
A community’s experience during and after the Ebola epidemic of 2014—2016 in Sierra Leone: A qualitative study
Authors: Murray Rianna T. , Drew Laura Briggs , Memmott Christina , Bangura Ya-Maila , Maring Elisabeth F.
Published: February 2021

Background

The 2014–2016 Ebola epidemic devastated families and communities throughout West Africa. Due to its high mortality rate and infectious nature, most Ebola research to date has focused on healthcare response and interventions; however, little is known about the experiences of Ebola survivors and communities. This qualitative study aimed to better understand the lived experiences of community members, including children, during and after the Ebola epidemic in Sierra Leone.

Methods

During June 2016 and June 2017, we conducted four focus groups comprised of primary school students, female caretakers, male caretakers, and teachers, and two individual in-depth interviews with local nurses in Calaba Town, a small village outside of Freetown. Interviews were recorded, transcribed verbatim, and coded using a modified grounded theory methodology.

Findings

All participants shared that they experienced significant challenges during and after the Ebola epidemic. During the epidemic, participants endured daily life challenges pertaining to fear, financial distress, and school closures. They also experienced suffering, loss, isolation, grief, and compromised culture. Confusion and distrust were also prevalent during the epidemic, with participants reporting confusion around Ebola transmission and distrust in the government and healthcare services. We also found that the struggle for food and grief stemming from the loss of loved ones continued more than a year after the epidemic ended. Despite Sierra Leone being declared Ebola-free, stigma and fear persisted and community members shared their continuing distrust of the government due to their actions during and after the epidemic.

Conclusions

The findings of this qualitative study reveal that the Ebola epidemic was a traumatizing period for the Calaba Town community, and that confusion and distrust toward the government health care system have continued. Future studies should explore the extended impact of the epidemic on communities, including long-term psychological, social, and economic consequences of this outbreak.
Selection of internal references for RT-qPCR assays in Neurofibromatosis type 1 (NF1) related Schwann cell lines
Authors: Gu Yi-Hui , Cui Xi-Wei , Ren Jie-Yi , Long Man-Mei , Wang Wei , Wei Cheng-Jiang , Aimaier Rehanguli , Li Yue-Hua , Chung Man-Hon , Gu Bin , Li Qing-Feng , Wang Zhi-Chao
Published: February 2021

Real-time quantitative PCR (RT-qPCR) has been widely applied in uncovering disease mechanisms and screening potential biomarkers. Internal reference gene selection determines the accuracy and reproducibility of data analyses. The aim of this study was to identify the optimal reference genes for the relative quantitative analysis of RT-qPCR in fourteen NF1 related cell lines, including non-tumor, benign and malignant Schwann cell lines. The expression characteristics of eleven candidate reference genes (RPS18, ACTB, B2M, GAPDH, PPIA, HPRT1, TBP, UBC, RPLP0, TFRC and RPL32) were screened and analyzed by four software programs: geNorm, NormFinder, BestKeeper and RefFinder. Results showed that GAPDH, the most frequently used internal reference gene, was significantly unstable between various cell lines. The combinational use of two reference genes (PPIA and TBP) was optimal in malignant Schwann cell lines and the use of single reference genes (PPIA or PRLP0) alone or in combination was optimal in benign Schwann cell lines. These recommended internal reference gene selections may improve the accuracy and reproducibility of RT-qPCR in gene expression analyses of NF1 related tumors.
Liver epigenome changes in patients with hepatopulmonary syndrome: A pilot study
Authors: Mendoza Nuria , Rivas Eva , Rodriguez-Roisin Roberto , Garcia Tamara , Bruguera Miquel , Agusti Alvar , Faner Rosa
Published: February 2021

The hepatopulmonary syndrome (HPS) is defined by the presence of pulmonary gas exchange abnormalities due to intrapulmonary vascular dilatations in patients with chronic liver disease. Changes in DNA methylation reflect the genomic variation. Since liver transplant (LT) reverts HPS we hypothesized that it may be associated with specific liver epigenetic changes. Thus, the aim of this study was to investigate the role of the liver epigenome in patients with HPS. We extracted DNA from paraffin embedded liver tissue samples from 10 patients with HPS and 10 age-, sex- and MELD (Model for End-stage Liver Disease)-matched controls. DNA methylation was determined using the 850K array (Illumina). Weighted Gene Co-expression Network Analysis (WGCNA) was used to identify modules related to defining physiologic characteristics of HPS. Only 12 out of the 20 liver biopsies (7 HPS and 5 controls) had sufficient quality to be analyzed. None of the 802,688 DNA probes analyzed in the case control comparison achieved a significant False Discovery Rate (FDR). WGCNA identified 5 co-methylated gene-modules associated to HPS markers, mainly related to nervous and neuroendocrine system, apoptotic processes, gut bacterial translocation, angiogenesis and vascular remodeling ontologies. To conclude, HPS is associated with nervous/neuroendocrine system and vascular remodeling related liver epigenetic changes.
Multi-criteria decision making in robotic agri-farming with q-rung orthopair m-polar fuzzy sets
Authors: Riaz Muhammad , Hamid Muhammad Tahir , Afzal Deeba , Pamucar Dragan , Chu Yu-Ming
Published: February 2021

q-Rung orthopair fuzzy set (qROFS) and m-polar fuzzy set (mPFS) are rudimentary concepts in the computational intelligence, which have diverse applications in fuzzy modeling and decision making under uncertainty. The aim of this paper is to introduce the hybrid concept of q-rung orthopair m-polar fuzzy set (qROmPFS) as a hybrid model of q-rung orthopair fuzzy set and m-polar fuzzy set. A qROmPFS has the ability to deal with real life situations when decision experts are interested to deal with multi-polarity as well as membership and non-membership grades to the alternatives in an extended domain with q-ROF environment. Certain operations on qROmPFSs and several new notions like support, core, height, concentration, dilation, α-cut and (α, β)-cut of qROmPFS are defined. Additionally, grey relational analysis (GRA) and choice value method (CVM) are presented under qROmPFSs for multi-criteria decision making (MCDM) in robotic agri-farming. The proposed methods are suitable to find out an appropriate mode of farming among several kinds of agri-farming. The applications of proposed MCDM approaches are illustrated by respective numerical examples. To justify the feasibility, superiority and reliability of proposed techniques, the comparison analysis of the final ranking in the robotic agri-farming computed by the proposed techniques with some existing MCDM methods is also given.
Revealing posturographic profile of patients with Parkinsonian syndromes through a novel hypothesis testing framework based on machine learning
Authors: Bargiotas Ioannis , Kalogeratos Argyris , Limnios Myrto , Vidal Pierre-Paul , Ricard Damien , Vayatis Nicolas
Published: February 2021

Falling in Parkinsonian syndromes (PS) is associated with postural instability and consists a common cause of disability among PS patients. Current posturographic practices record the body’s center-of-pressure displacement (statokinesigram) while the patient stands on a force platform. Statokinesigrams, after appropriate processing, can offer numerous posturographic features. This fact, although beneficial, challenges the efforts for valid statistics via standard univariate approaches. In this work, 123 PS patients were classified into fallers (PSF) or non-faller (PSNF) based on the clinical assessment, and underwent simple Romberg Test (eyes open/eyes closed). We developed a non-parametric multivariate two-sample test (ts-AUC) based on machine learning, in order to examine statokinesigrams’ differences between PSF and PSNF. We analyzed posturographic features using both multiple testing with p-value adjustment and ts-AUC. While ts-AUC showed significant difference between groups (p-value = 0.01), multiple testing did not agree with this result (eyes open). PSF showed significantly increased antero-posterior movements as well as increased posturographic area compared to PSNF. Our study highlights the superiority of ts-AUC compared to standard statistical tools in distinguishing PSF and PSNF in multidimensional space. Machine learning-based statistical tests can be seen as a natural extension of classical statistics and should be considered, especially when dealing with multifactorial assessments.
SNAP timing and food insecurity
Authors: Gregory Christian A. , Todd Jessica E.
Published: February 2021

This paper makes several contributions to the literature regarding the measurement of food insecurity and implications for estimating factors that affect this outcome. First, we show that receipt of benefits from the Supplemental Nutrition Assistance Program (SNAP) has a systematic effect on responses to questions in the 12-month food security module (FSM). We find that the probability of affirming more severe food hardships items, and the probability of being classified as having very low food security (VLFS), is higher just before and just after households receive their benefits. This leads to an under-estimate of VLFS by 3.2 percentage points for the SNAP sample (about 17 percent of prevalence). We also provide informative bounds on the relationship between SNAP and VLFS and show that the treatment effect of SNAP on VLFS is also likely underestimated.
Childhood trauma and cognitive biases associated with psychosis: A systematic review and meta-analysis
Authors: Croft Jazz , Martin David , Madley-Dowd Paul , Strelchuk Daniela , Davies Jonathan , Heron Jon , Teufel Christoph , Zammit Stanley
Published: February 2021

Childhood trauma is associated with an increased risk of psychosis, but the mechanisms that mediate this relationship are unknown. Exposure to trauma has been hypothesised to lead to cognitive biases that might have causal effects on psychotic symptoms. The literature on whether childhood trauma is associated with psychosis-related cognitive biases has not been comprehensively reviewed. A systematic review and meta-analysis or narrative synthesis of studies examining the association between childhood trauma and the following biases: external locus of control (LOC), external attribution, probabilistic reasoning, source monitoring, top-down processing, and bias against disconfirmatory evidence. Studies were assessed for quality, and sources of heterogeneity were explored. We included 25 studies from 3,465 studies identified. Individuals exposed to childhood trauma reported a more external LOC (14 studies: SMD Median = 0.40, Interquartile range 0.07 to 0.52), consistent with a narrative synthesis of 11 other studies of LOC. There was substantial heterogeneity in the meta-analysis (I2 = 93%) not explained by study characteristics examined. Narrative syntheses for other biases showed weaker, or no evidence of association with trauma. The quality of included studies was generally low. Our review provides some evidence of an association between childhood trauma and a more external LOC, but not with the other biases examined. The low quality and paucity of studies for most of the cognitive biases examined highlights the need for more rigorous studies to determine which biases occur after trauma, and whether they mediate an effect of childhood trauma on psychosis.
A “ligand-targeting” peptide-drug conjugate: Targeted intracellular drug delivery by VEGF-binding helix-loop-helix peptides via receptor-mediated endocytosis
Authors: Michigami Masataka , Takahashi Kentaro , Yamashita Haruna , Ye Zhengmao , Nakase Ikuhiko , Fujii Ikuo
Published: February 2021

As a new alternative to antibody-drug conjugates, we generated “ligand-targeting” peptide-drug conjugates (PDCs), which utilize receptor-mediated endocytosis for targeted intracellular drug delivery. The PDC makes a complex with an extracellular ligand and then binds to the receptor on the cell surface to stimulate intracellular uptake via the endocytic pathway. A helix-loop-helix (HLH) peptide was designed as the drug carrier and randomized to give a conformationally constrained peptide library. The phage-displayed library was screened against vascular endothelial growth factor (VEGF) to yield the binding peptide M49, which exhibited strong binding affinity (KD= 0.87 nM). The confocal fluorescence microscopy revealed that peptide M49 formed a ternary complex with VEGF and its receptor, which was then internalized into human umbilical vein endothelial cells (HUVECs) via VEGF receptor-mediated endocytosis. The backbone-cyclized peptide M49K was conjugated with a drug, monomethyl auristatin E, to afford a PDC, which inhibited VEGF-induced HUVEC proliferation. HLH peptides and their PDCs have great potential as a new modality for targeted molecular therapy.
Determinants of intention to improve oral hygiene behavior among students based on the theory of planned behavior: A structural equation modelling analysis
Authors: Shitu Kegnie , Alemayehu Mekuriaw , Buunk-Werkhoven Yvonne A. B. , Handebo Simegnew
Published: February 2021

Introduction

The prevalence of oral hygiene behaviors (OHB) is very low among school children in Ethiopia. However, the determinants of student’s readiness/intention to perform those behaviors have been remained unstudied.

Objective

This study aimed to identify the determinants of oral hygiene behavioral intention (OHBI) among preparatory school students based on the theory of planned behavior (TPB).

Methods and materials

An institution-based cross-sectional study was conducted among 393 students. A 98-item self-administered questionnaire was used to evaluate oral hygiene knowledge (OHK), oral hygiene behavior (OHB), and OHBI based on TPB variables [attitude (ATT), subjective norms (SN) and perceived behavioral control (PBC)]. Descriptive statistics and structural equation modeling analysis (SEM) were employed to confirm relationships and associations among study variables. A p-value of less than 0.05 and a 95% confidence interval were used to declare statistical significance.

Results

A total of 393 students were participated with a response rate of 97.5%. The mean age of the participants (54% females) was 18 (± 1.3) with an age range of 16 to 24. The TPB model was well fitted to the data and explained 66% of the variance in intention. ATT (β = 0.38; 95% CI, (0.21, 0.64)), SN (β = 0.33; 95% CI, (0.05, 0.83)) and PBC (β = 0.29; 95% CI, (0.13, 0.64)) were significant predictors of OHBI, where ATT was the strongest predictor of OHBI.

Conclusion

The TPB model explained a large variance in the intention of students to improve their OHB. All TPB variables were significantly and positively linked to stronger intent, as the theory suggests. Furthermore, these results suggest that the model could provide a framework for oral hygiene promotion interventions in the study area. Indeed, these interventions should focus on changing the attitudes of students towards OHB, creation of positive social pressure, and enabling students to control over OHB barriers.
A novel methodology to study antimicrobial properties of high-touch surfaces used for indoor hygiene applications—A study on Cu metal
Authors: Chang T. , Sepati M. , Herting G. , Leygraf C. , Rajarao G. Kuttuva , Butina K. , Richter-Dahlfors A. , Blomberg E. , Odnevall Wallinder I.
Published: February 2021

Metal-based high-touch surfaces used for indoor applications such as doorknobs, light switches, handles and desks need to remain their antimicrobial properties even when tarnished or degraded. A novel laboratory methodology of relevance for indoor atmospheric conditions and fingerprint contact has therefore been elaborated for combined studies of both tarnishing/corrosion and antimicrobial properties of such high-touch surfaces. Cu metal was used as a benchmark material. The protocol includes pre-tarnishing/corrosion of the high touch surface for different time periods in a climatic chamber at repeated dry/wet conditions and artificial sweat deposition followed by the introduction of bacteria onto the surfaces via artificial sweat droplets. This methodology provides a more realistic and reproducible approach compared with other reported procedures to determine the antimicrobial efficiency of high-touch surfaces. It provides further a possibility to link the antimicrobial characteristics to physical and chemical properties such as surface composition, chemical reactivity, tarnishing/corrosion, surface roughness and surface wettability. The results elucidate that bacteria interactions as well as differences in extent of tarnishing can alter the physical properties (e.g. surface wettability, surface roughness) as well as the extent of metal release. The results clearly elucidate the importance to consider changes in chemical and physical properties of indoor hygiene surfaces when assessing their antimicrobial properties.
Fatigue following COVID-19 infection is not associated with autonomic dysfunction
Authors: Townsend Liam , Moloney David , Finucane Ciaran , McCarthy Kevin , Bergin Colm , Bannan Ciaran , Kenny Rose-Anne
Published: February 2021

Background

The long-term clinical and physiological consequences of COVID-19 infection remain unclear. While fatigue has emerged as a common symptom following infection, little is known about its links with autonomic dysfunction. SARS-CoV-2 is known to infect endothelial cells in acute infection, resulting in autonomic dysfunction. Here we set out to test the hypothesis that this results in persistent autonomic dysfunction and is associated with post-COVID fatigue in convalescent patients.

Methods

We recruited 20 fatigued and 20 non-fatigued post-COVID patients (median age 44.5 years, 36/40 (90%) female, median time to follow up 166.5 days). Fatigue was assessed using the Chalder Fatigue Scale. These underwent the Ewing’s autonomic function test battery, including deep breathing, active standing, Valsalva manoeuvre and cold-pressor testing, with continuous electrocardiogram and blood pressure monitoring, as well as near-infrared spectroscopy-based cerebral oxygenation. 24-hour ambulatory blood pressure monitoring was also conducted, and patients completed the generalised anxiety disorder-7 questionnaire. We assessed between-group differences in autonomic function test results and used unadjusted and adjusted linear regression to investigate the relationship between fatigue, anxiety, and autonomic test results.

Results

We found no pathological differences between fatigued and non-fatigued patients on autonomic testing or on 24-hour blood pressure monitoring. Symptoms of orthostatic intolerance were reported by 70% of the fatigued cohort at the time of active standing, with no associated physiological abnormality detected. Fatigue was strongly associated with increased anxiety (p <0.001), with no patients having a pre-existing diagnosis of anxiety.

Conclusions

These results demonstrate the significant burden of fatigue, symptoms of autonomic dysfunction and anxiety in the aftermath of COVID-19 infection, but reassuringly do not demonstrate pathological findings on autonomic testing.
Awareness of and willingness to use pre-exposure prophylaxis (PrEP) among people who inject drugs and men who have sex with men in India: Results from a multi-city cross-sectional survey
Authors: Belludi Ashwin , McFall Allison M. , Solomon Sunil Suhas , Celentano David D. , Mehta Shruti H. , Srikrishnan A. K. , Kumar M. Suresh , Solomon Suniti , Lucas Gregory M.
Published: February 2021

Introduction

Pre-exposure prophylaxis (PrEP) is effective in reducing HIV transmission among key populations. In India, where PrEP is not currently part of the national HIV program, little is known about PrEP awareness, willingness to use PrEP, and barriers to uptake among people who inject drugs (PWID) and men who have sex with men (MSM).

Methods

We used respondent-driven sampling to accrue PWID and MSM in 22 sites from August 2016 to May 2017. Participants were asked about awareness of PrEP, willingness to use PrEP (following a brief description) and reasons why they might not be willing to use PrEP. Participants were also queried on preferences for PrEP delivery modality (oral vs. injectable). Multi-level logistic regression models were used to determine participant correlates of willingness to use PrEP. Estimates were weighted for the sampling method.

Results

A total of 10,538 PWID and 8,621 MSM who self-reported being HIV-negative were included in the analysis. Only 6.1% (95% confidence interval [CI]: 5.9, 6.3) of PWID and 8.0% of MSM (95% CI: 7.7, 8.4) were aware of PrEP. However, willingness to use PrEP was substantially higher in both groups: 52.4% of PWID and 67.6% of MSM. Participants commonly cited a perceived low risk for acquiring HIV infection, being perceived by others as being HIV-positive, and side effects as reasons why they would be unwilling to use PrEP. Among PWID, sharing needles and hazardous alcohol use were associated with increased willingness to use PrEP. Among MSM, having a main male partner and injection drug use were associated with increased willingness to use PrEP. Preference for daily oral or monthly injectable PrEP was similar among MSM (39.6%% vs. 41.7%,), while PWID were more likely to prefer oral to injectable administration routes (56.3% vs. 31.1%).

Conclusions

As India plans to roll-out of PrEP in the public sector, our multi-city survey of PWID and MSM highlights the need for key population-focused education campaigns about PrEP and self-assessment of risk.
A dataset of human and robot approach behaviors into small free-standing conversational groups
Authors: Yang Fangkai , Gao Yuan , Ma Ruiyang , Zojaji Sahba , Castellano Ginevra , Peters Christopher
Published: February 2021

The analysis and simulation of the interactions that occur in group situations is important when humans and artificial agents, physical or virtual, must coordinate when inhabiting similar spaces or even collaborate, as in the case of human-robot teams. Artificial systems should adapt to the natural interfaces of humans rather than the other way around. Such systems should be sensitive to human behaviors, which are often social in nature, and account for human capabilities when planning their own behaviors. A limiting factor relates to our understanding of how humans behave with respect to each other and with artificial embodiments, such as robots. To this end, we present CongreG8 (pronounced ‘con-gre-gate’), a novel dataset containing the full-body motions of free-standing conversational groups of three humans and a newcomer that approaches the groups with the intent of joining them. The aim has been to collect an accurate and detailed set of positioning, orienting and full-body behaviors when a newcomer approaches and joins a small group. The dataset contains trials from human and robot newcomers. Additionally, it includes questionnaires about the personality of participants (BFI-10), their perception of robots (Godspeed), and custom human/robot interaction questions. An overview and analysis of the dataset is also provided, which suggests that human groups are more likely to alter their configuration to accommodate a human newcomer than a robot newcomer. We conclude by providing three use cases that the dataset has already been applied to in the domains of behavior detection and generation in real and virtual environments.

A sample of the CongreG8 dataset is available at https://zenodo.org/record/4537811.
Application of biochar from crop straw in asphalt modification
Authors: Gan Xinli , Zhang Wenli
Published: February 2021

The objective of this study is to verify the feasibility of using biochar made from crop straw as a bitumen additive to improve some properties of bitumen. The differences between crop straw biochar prepared in a laboratory and commercial charcoal were investigated through scanning electron microscopy and laser particle size analyses. Furthermore, biochar-modified asphalt was prepared using the high-speed shear method, and the penetration, softening point, ductility at 15°C, and apparent viscosity of the asphalt binder with 6% biochar were measured at 120, 135, 150, 160, and 175°C. It was found that both the crop straw biochar and the commercial charcoal consist mainly of C, O, Si, and K, but the C content of crop straw biochar is slightly higher than that of commercial charcoal. The particle size of biochar is smaller than that of commercial charcoal, while the specific surface area is larger. It was determined that the addition of crop straw biochar significantly improved the high-temperature performance of asphalt, and that biochar and commercial charcoal have a similar influence on the high temperature performance of asphalt.
Comparing different types of statins for secondary prevention of cardio-cerebrovascular disease from a national cohort study
Authors: Kim Joungyoun , Kim Hyeong-Seop , Bae Yun-Jong , Lee Hyeong-Chul , Lee Jae-woo , Kang Hee-Taik
Published: February 2021

Statins have been recommended for use in atherosclerotic cardio-cerebrovascular disease (CCVD). The purpose of this study was to investigate the efficacy of five different types of statin in the secondary prevention of CCVD in patients. This study retrospectively designed and analyzed data from the National Health Insurance Service-National Health in Korea. Participants aged 40 to 69 years were categorized into five statin groups (atorvastatin, rosuvastatin, pitavastatin, simvastatin, and pravastatin). The primary composite outcome was defined as recurrence of CCVD or all causes of death. Cox proportional hazard regression models were adopted after stepwise adjustments for confounders to investigate the difference in efficacy among the different statins. Of the 755 final participants, 48 patients experienced primary composite outcomes. After adjustments, the hazard ratios (95% confidence intervals) for primary composite outcomes of atorvastatin, pitavastatin, and rosuvastatin groups were 0.956 (0.456–2.005), 1.347 (0.354–5.116), and 0.943 (0.317–2.803), respectively, when compared with the simvastatin group. There were no significant differences between the statins in efficacy for preventing recurrence of CCVD events and/or death in CCVD patients.
Computational fluid dynamics of the right atrium: Assessment of modelling criteria for the evaluation of dialysis catheters
Authors: de Oliveira Diana C. , Owen David G. , Qian Shuang , Green Naomi C. , Espino Daniel M. , Shepherd Duncan E. T.
Published: February 2021

Central venous catheters are widely used in haemodialysis therapy, having to respect design requirements for appropriate performance. These are placed within the right atrium (RA); however, there is no prior computational study assessing different catheter designs while mimicking their native environment. Here, a computational fluid dynamics model of the RA, based on realistic geometry and transient physiological boundary conditions, was developed and validated. Symmetric, split and step catheter designs were virtually placed in the RA and their performance was evaluated by: assessing their interaction with the RA haemodynamic environment through prediction of flow vorticity and wall shear stress (WSS) magnitudes (1); and quantifying recirculation and tip shear stress (2). Haemodynamic predictions from our RA model showed good agreement with the literature. Catheter placement in the RA increased average vorticity, which could indicate alterations of normal blood flow, and altered WSS magnitudes and distribution, which could indicate changes in tissue mechanical properties. All designs had recirculation and elevated shear stress values, which can induce platelet activation and subsequently thrombosis. The symmetric design, however, had the lowest associated values (best performance), while step design catheters working in reverse mode were associated with worsened performance. Different tip placements also impacted on catheter performance. Our findings suggest that using a realistically anatomical RA model to study catheter performance and interaction with the haemodynamic environment is crucial, and that care needs to be given to correct tip placement within the RA for improved recirculation percentages and diminished shear stress values.