bluuebunny/crossref_metadata_2025 huggingface dataset:
Phytosynthesis of Palladium Nanoclusters: An Efficient Nanozyme for Ultrasensitive and Selective Detection of Reactive Oxygen Species
Authors: Tripathi Ravi Mani , Chung Sang J.
Published: July 2020

Hydrogen peroxide is a low-reactivity reactive oxygen species (ROS); however, it can easily penetrate cell membranes and produce highly reactive hydroxyl radical species through Fenton’s reaction. Its presence in abnormal amounts can lead to serious diseases in humans. Although the development of a simple, ultrasensitive, and selective method for H2O2 detection is crucial, this remains a strategic challenge. The peroxidase mimetic activity of palladium nanoclusters (PdNCs) has not previously been evaluated. In this study, we developed an ultrasensitive and selective colorimetric detection method for H2O2 using PdNCs. An unprecedented eco-friendly, cost-effective, and facile biological method was developed for the synthesis of PdNCs. This is the first report of the biosynthesis of PdNCs. The synthesized nanoclusters had a significantly narrow size distribution profile and high stability. The nanoclusters were demonstrated to possess a peroxidase mimetic activity that could oxidize peroxidase substrate 3,3′,5,5′-tetramethylbenzidine (TMB). Various interfering substances in serum (100 μM phenylalanine, cysteine, tryptophan, arginine, glucose, urea, Na+, Fe2+, PO43−, Mn+2, Ca2+, Mg2+, Zn2+, NH4+, and K+) were included to evaluate the selectivity of the assay, and oxidation of TMB occurred only in the presence of H2O2. Therefore, PdNCs show an efficient nanozyme for the peroxidase mimetic activity. The assay produced a sufficient signal at the ultralow concentration of 0.0625 µM H2O2. This colorimetric assay provides a real-time, rapid, and easy-to-use platform for the detection of H2O2 for clinical purposes.
Supramolecular Interactions in Hybrid Polylactide Blends—The Structures, Mechanisms and Properties
Authors: Kowalewska Anna , Nowacka Maria
Published: July 2020

The conformation of polylactide (PLA) chains can be adjusted by supramolecular interactions (the formation of hydrogen bonds or host-guest complexes) with appropriate organic molecules. The structures formed due to those intermolecular interactions may act as crystal nuclei in the PLA matrix (“soft templating”). In this review, the properties of several supramolecular nucleating systems based on synthetic organic nucleators (arylamides, hydrazides, and 1,3:2,4-dibenzylidene-d-sorbitol) are compared to those achieved with biobased nucleating agents (orotic acid, humic acids, fulvic acids, nanocellulose, and cyclodextrins) that can also improve the mechanical properties of PLA. The PLA nanocomposites containing both types of nucleating agents/additives are discussed and evaluated in the context of their biomedical applicability.
Flavonoid and Non-Flavonoid Compounds of Autumn Royal and Egnatia Grape Skin Extracts Affect Membrane PUFA’s Profile and Cell Morphology in Human Colon Cancer Cell Lines
Authors: Tutino Valeria , Gigante Isabella , Milella Rosa Anna , De Nunzio Valentina , Flamini Riccardo , De Rosso Mirko , Scavo Maria Principia , Depalo Nicoletta , Fanizza Elisabetta , Caruso Maria Gabriella , Notarnicola Maria
Published: July 2020

Grapes contain many flavonoid and non-flavonoid compounds with anticancer effects. In this work we fully characterized the polyphenolic profile of two grape skin extracts (GSEs), Autumn Royal and Egnatia, and assessed their effects on Polyunsaturated Fatty Acid (PUFA) membrane levels of Caco2 and SW480 human colon cancer cell lines. Gene expression of 15-lipoxygenase-1 (15-LOX-1), and peroxisome proliferator-activated receptor gamma (PPAR-γ), as well as cell morphology, were evaluated. The polyphenolic composition was analyzed by Ultra-High-Performance Liquid Chromatography/Quadrupole-Time of Flight mass spectrometry (UHPLC/QTOF) analysis. PUFA levels were evaluated by gas chromatography, and gene expression levels of 15-LOX-1 and PPAR-γ were analyzed by real-time Polymerase Chain Reaction (PCR). Morphological cell changes caused by GSEs were identified by field emission scanning electron microscope (FE-SEM) and photomicrograph examination. We detected a different profile of flavonoid and non-flavonoid compounds in Autumn Royal and Egnatia GSEs. Cultured cells showed an increase of total PUFA levels mainly after treatment with Autumn Royal grape, and were richer in flavonoids when compared with the Egnatia variety. Both GSEs were able to affect 15-LOX-1 and PPAR-γ gene expression and cell morphology. Our results highlighted a new antitumor mechanism of GSEs that involves membrane PUFAs and their downstream pathways.
Nanosized Pinning Centers in the Rare Earth-Barium-Copper-Oxide Thin-Film Superconductors
Authors: Antončík Filip , Jankovský Ondřej , Hlásek Tomáš , Bartůněk Vilém
Published: July 2020

Since the discovery of high-temperature superconductivity, significant progress in the fabrication of REBCO-based (Rare Earth Barium Copper mixed Oxides) thin-films superconductors has been achieved. In our review, we described the approaches and possibilities of the improvement of superconducting properties by the introduction of nanosized pinning centers. We focused on the synthesis and viability of the material for artificial pinning centers and methods used for the introduction of the pinning centers into superconducting REBCO-based thin-films. This article summarizes available materials and procedures regardless of the financial cost of the individual method. According to available literature, the most significant superconducting REBCO tapes can be obtained when a combination of 1D and 0D nanoparticles are used for nanoscale pinning.
Bandgap-Tuned 2D Boron Nitride/Tungsten Nitride Nanocomposites for Development of High-Performance Deep Ultraviolet Selective Photodetectors
Authors: Aldalbahi Ali , Velázquez Rafael , Zhou Andrew F. , Rahaman Mostafizur , Feng Peter X.
Published: July 2020

This study presents a fast and effective method to synthesize 2D boron nitride/tungsten nitride (BN–WN) nanocomposites for tunable bandgap structures and devices. A few minutes of synthesis yielded a large quantity of high-quality 2D nanocomposites, with which a simple, low-cost deep UV photo-detector (DUV-PD) was fabricated and tested. The new device was demonstrated to have very good performance. High responsivity up to 1.17 A/W, fast response-time of lower than two milliseconds and highly stable repeatability were obtained. Furthermore, the influences of operating temperature and applied bias voltage on the properties of DUV-PD as well as its band structure shift were investigated.
Electrodeposition of Mesoporous Ni-Rich Ni-Pt Films for Highly Efficient Methanol Oxidation
Authors: Artal Raül , Serrà Albert , Michler Johann , Philippe Laëtitia , Gómez Elvira
Published: July 2020

The use of soft templates for the electrosynthesis of mesoporous materials has shown tremendous potential in energy and environmental domains. Among all the approaches that have been featured in the literature, block copolymer-templated electrodeposition had robustness and a simple method, but it practically cannot be used for the synthesis of mesoporous materials not based on Pt or Au. Nonetheless, extending and understanding the possibilities and limitations of block copolymer-templated electrodeposition to other materials and substrates is still challenging. Herein, a critical analysis of the role of the solution’s primary electroactive components and the applied potential were performed in order to understand their influences on the mesostructure of Ni-rich Ni-Pt mesoporous films. Among all the components, tetrahydrofuran and a platinum (IV) complex were shown to be crucial for the formation of a truly 3D mesoporous network. The electrosynthesized well-ordered mesoporous Ni-rich Ni-Pt deposits exhibit excellent electrocatalytic performance for methanol oxidation in alkaline conditions, improved stability and durability after 1000 cycles, and minimal CO poisoning.
An Investigative Study on the Effect of Pre-Coating Polymer Solutions on the Fabrication of Low Cost Anti-Adhesive Release Paper
Authors: Vasilev Semen , Vodyashkin Andrey , Vasileva Daria , Zelenovskiy Pavel , Chezganov Dmitry , Yuzhakov Vladimir , Shur Vladimir , O’Reilly Emmet , Vinogradov Alexandr
Published: July 2020

This work describes a novel approach to produce high quality release paper at lower cost than traditional methods. The anti-adhesive properties of release paper require the use of expensive machine glazed kraft or “Glassine” paper as the paper base. A series of polymer coatings including polyvinyl alcohol, carboxymethyl cellulose, polyethylene vinyl acetate, and polystyrene were chemically synthesized and coated onto a low cost pulp paper base. Surface roughness (Sa) and smoothness coefficients (k) were determined by atomic force microscopy (AFM), and the interactions between the polymer coating and base paper were investigated by Raman spectroscopy. Studies show the use of polyethylene vinyl acetate (PEVA) as a pre-coating layer on low cost pulp paper exhibits similar anti-adhesive properties as higher cost paper bases. In low margin markets such as the production of release paper, decreases in cost are critical to industry survival.
Forward and Backward Unidirectional Scattering by the Core-Shell Nanocube Dimer with Balanced Gain and Loss
Authors: Lv Jingwei , Zhang Xiaoming , Yu Xuntao , Mu Haiwei , Liu Qiang , Liu Chao , Sun Tao , Chu Paul K.
Published: July 2020

An optical nanoantenna consisting of a Au-dielectric core-shell nanocube dimer with switchable directionality was designed and described. Our theoretical model and numerical simulation showed that switching between forward and backward directions can be achieved with balanced gain and loss, using a single element by changing the coefficient κ in the core, which can be defined by the relative phase of the polarizability. The optical response indicated a remarkable dependence on the coefficient κ in the core as well as frequency. The location of the electric field enhancement was specified by the different coefficient κ and, furthermore, the chained optical nanoantenna and coupled electric dipole emitted to the optical nanoantenna played significant roles in unidirectional scattering. This simple method to calculate the feasibility of unidirectional and switchable scattering provides an effective strategy to explore the functionalities of nanophotonic devices.
Extending the Overnight Fast: Sex Differences in Acute Metabolic Responses to Breakfast
Authors: S. Atkinson Fiona , A. Heruc Gabriella , M. H. Tan Verena , Petocz Peter , C. Brand-Miller Jennie
Published: July 2020

Fasting for over 24 h is associated with worsening glucose tolerance, but the effect of extending the overnight fast period (a form of time-restricted feeding) on acute metabolic responses and insulin sensitivity is unclear. The aim of this pilot study was to determine the acute impact of an increased fasting period on postprandial glycaemia, insulinemia, and acute insulin sensitivity responses to a standard meal. Twenty-four lean, young, healthy adults (12 males, 12 females) consumed a standard breakfast after an overnight fast of 12, 14, and 16 h. Each fast duration was repeated on three separate occasions (3 × 3) in random order. Postprandial glucose and insulin responses were measured at regular intervals over 2 h and quantified as incremental area under the curve (iAUC). Insulin sensitivity was determined by homeostatic modelling assessment (HOMA). After 2 h, ad libitum food intake at a buffet meal was recorded. In females, but not males, insulin sensitivity improved (HOMA%S +35%, p = 0.016, marginally significant) with longer fast duration (16 h vs. 12 h), but paradoxically, postprandial glycaemia was higher (glucose iAUC +37%, p = 0.002). Overall, males showed no differences in glucose or insulin homeostasis. Both sexes consumed more energy (+28%) at the subsequent meal (16 h vs. 12 h). Delaying the first meal of the day by 4 h by extending the fasting period may have adverse metabolic effects in young, healthy, adult females, but not males.
The Importance of the Fatty Acid Transporter L-Carnitine in Non-Alcoholic Fatty Liver Disease (NAFLD)
Authors: Savic Dragana , Hodson Leanne , Neubauer Stefan , Pavlides Michael
Published: July 2020

L-carnitine transports fatty acids into the mitochondria for oxidation and also buffers excess acetyl-CoA away from the mitochondria. Thus, L-carnitine may play a key role in maintaining liver function, by its effect on lipid metabolism. The importance of L-carnitine in liver health is supported by the observation that patients with primary carnitine deficiency (PCD) can present with fatty liver disease, which could be due to low levels of intrahepatic and serum levels of L-carnitine. Furthermore, studies suggest that supplementation with L-carnitine may reduce liver fat and the liver enzymes alanine aminotransferase (ALT) and aspartate transaminase (AST) in patients with Non-Alcoholic Fatty Liver Disease (NAFLD). L-carnitine has also been shown to improve insulin sensitivity and elevate pyruvate dehydrogenase (PDH) flux. Studies that show reduced intrahepatic fat and reduced liver enzymes after L-carnitine supplementation suggest that L-carnitine might be a promising supplement to improve or delay the progression of NAFLD.
Adherence to Mediterranean Diet and Maternal Lifestyle during Pregnancy: Island–Mainland Differentiation in the CRIBS Birth Cohort
Authors: Havaš Auguštin Dubravka , Šarac Jelena , Lovrić Mario , Živković Jelena , Malev Olga , Fuchs Nives , Novokmet Natalija , Turkalj Mirjana , Missoni Saša
Published: July 2020

Maternal nutrition and lifestyle in pregnancy are important modifiable factors for both maternal and offspring’s health. Although the Mediterranean diet has beneficial effects on health, recent studies have shown low adherence in Europe. This study aimed to assess the Mediterranean diet adherence in 266 pregnant women from Dalmatia, Croatia and to investigate their lifestyle habits and regional differences. Adherence to the Mediterranean diet was assessed through two Mediterranean diet scores. Differences in maternal characteristics (diet, education, income, parity, smoking, pre-pregnancy body mass index (BMI), physical activity, contraception) with regards to location and dietary habits were analyzed using the non-parametric Mann–Whitney U test. The machine learning approach was used to reveal other potential non-linear relationships. The results showed that adherence to the Mediterranean diet was low to moderate among the pregnant women in this study, with no significant mainland–island differences. The highest adherence was observed among wealthier women with generally healthier lifestyle choices. The most significant mainland–island differences were observed for lifestyle and socioeconomic factors (income, education, physical activity). The machine learning approach confirmed the findings of the conventional statistical method. We can conclude that adverse socioeconomic and lifestyle conditions were more pronounced in the island population, which, together with the observed non-Mediterranean dietary pattern, calls for more effective intervention strategies.
Impact of Formate Supplementation on Body Weight and Plasma Amino Acids
Authors: Dhayade Sandeep , Pietzke Matthias , Wiesheu Robert , Tait-Mulder Jacqueline , Athineos Dimitris , Sumpton David , Coffelt Seth , Blyth Karen , Vazquez Alexei
Published: July 2020

Current nutritional recommendations are focused on energy, fat, carbohydrate, protein and vitamins. Less attention has been paid to the nutritional demand of one-carbon units for nucleotide and methionine synthesis. Here, we investigated the impact of sodium formate supplementation as a nutritional intervention to increase the dietary intake of one-carbon units. A cohort of six female and six male mice received 125 mM of sodium formate in the drinking water for three months. A control group of another six female and six male mice was also followed up for the same period of time. Tail vein blood samples were collected once a month and profiled with a haematology analyser. At the end of the study, blood and tissues were collected for metabolomics analysis and immune cell profiling. Formate supplementation had no significant physiological effect on male mice, except for a small decrease in body weight. Formate supplementation had no significant effect on the immune cell counts during the intervention or at the end of the study in either gender. In female mice, however, the body weight and spleen wet weight were significantly increased by formate supplementation, while the blood plasma levels of amino acids were decreased. Formate supplementation also increased the frequency of bifidobacteria, a probiotic bacterium, in the stools of female mice. We conclude that formate supplementation induces physiological changes in a gender-specific manner.
No Effect of Diet-Induced Mild Hyperhomocysteinemia on Vascular Methylating Capacity, Atherosclerosis Progression, and Specific Histone Methylation
Authors: Whalen Courtney A. , Mattie Floyd J. , Florindo Cristina , van Zelst Bertrand , Huang Neil K. , Tavares de Almeida Isabel , Heil Sandra G. , Neuberger Thomas , Ross A. Catharine , Castro Rita
Published: July 2020

Hyperhomocysteinemia (HHcy) is a risk factor for atherosclerosis through mechanisms which are still incompletely defined. One possible mechanism involves the hypomethylation of the nuclear histone proteins to favor the progression of atherosclerosis. In previous cell studies, hypomethylating stress decreased a specific epigenetic tag (the trimethylation of lysine 27 on histone H3, H3K27me3) to promote endothelial dysfunction and activation, i.e., an atherogenic phenotype. Here, we conducted a pilot study to investigate the impact of mild HHcy on vascular methylating index, atherosclerosis progression and H3K27me3 aortic content in apolipoprotein E-deficient (ApoE −/−) mice. In two different sets of experiments, male mice were fed high-fat, low in methyl donors (HFLM), or control (HF) diets for 16 (Study A) or 12 (Study B) weeks. At multiple time points, plasma was collected for (1) quantification of total homocysteine (tHcy) by high-performance liquid chromatography; or (2) the methylation index of S-adenosylmethionine to S-adenosylhomocysteine (SAM:SAH ratio) by liquid chromatography tandem-mass spectrometry; or (3) a panel of inflammatory cytokines previously implicated in atherosclerosis by a multiplex assay. At the end point, aortas were collected and used to assess (1) the methylating index (SAM:SAH ratio); (2) the volume of aortic atherosclerotic plaque assessed by high field magnetic resonance imaging; and (3) the vascular content of H3K27me3 by immunohistochemistry. The results showed that, in both studies, HFLM-fed mice, but not those mice fed control diets, accumulated mildly elevated tHcy plasmatic concentrations. However, the pattern of changes in the inflammatory cytokines did not support a major difference in systemic inflammation between these groups. Accordingly, in both studies, no significant differences were detected for the aortic methylating index, plaque burden, and H3K27me3 vascular content between HF and HFLM-fed mice. Surprisingly however, a decreased plasma SAM: SAH was also observed, suggesting that the plasma compartment does not always reflect the vascular concentrations of these two metabolites, at least in this model. Mild HHcy in vivo was not be sufficient to induce vascular hypomethylating stress or the progression of atherosclerosis, suggesting that only higher accumulations of plasma tHcy will exhibit vascular toxicity and promote specific epigenetic dysregulation.
Maternal High Linoleic Acid Alters Placental Fatty Acid Composition
Authors: Shrestha Nirajan , Holland Olivia J. , Kent Nykola L. , Perkins Anthony V. , McAinch Andrew J. , Cuffe James S. M. , Hryciw Deanne H.
Published: July 2020

Fetal development is modulated by maternal nutrition during pregnancy. The dietary intake of linoleic acid (LA), an essential dietary n-6 polyunsaturated fatty acid (PUFA), has increased. We previously published that increased LA consumption during pregnancy does not alter offspring or placental weight but fetal plasma fatty acid composition; the developing fetus obtains their required PUFA from the maternal circulation. However, it is unknown if increased maternal linoleic acid alters placental fatty acid storage, metabolism, transport, and general placental function. Female Wistar-Kyoto rats were fed either a low LA diet (LLA; 1.44% of energy from LA) or high LA diet (HLA; 6.21% of energy from LA) for 10 weeks before pregnancy and during gestation. Rats were sacrificed at embryonic day 20 (E20, term = 22 days) and placentae collected. The labyrinth of placentae from one male and one female fetus from each litter were analyzed. High maternal LA consumption increased placental total n-6 and LA concentrations, and decreased total n-3 PUFA, alpha-linolenic acid (ALA), and docosahexaenoic acid (DHA). Fatty acid desaturase 1 (Fads1), angiopoietin-like 4 (Angptl4), and diacylglycerol lipase beta (Daglb) mRNA were downregulated in placentae from offspring from HLA dams. Maternal high LA downregulated the fatty acid transport protein 4 (Fatp4) and glucose transporter 1 (Slc2a1) mRNA in placentae. IL-7 and IL-10 protein were decreased in placentae from offspring from HLA dams. In conclusion, a high maternal LA diet alters the placental fatty acid composition, inflammatory proteins, and expressions of nutrient transporters, which may program deleterious outcomes in offspring.
Natural Fermentation of Cowpea (Vigna unguiculata) Flour Improves the Nutritive Utilization of Indispensable Amino Acids and Phosphorus by Growing Rats
Authors: Kapravelou Garyfallia , Martínez Rosario , Martino Jole , Porres Jesus , Fernández-Fígares Ignacio
Published: July 2020

Cowpea (Vigna unguiculata) is among the most cultivated legumes, with interesting agronomic and environmental properties, and great potential as a nutritious food. The nutritional value of cowpea can be improved by technological processing. In this study, we showed that natural fermentation improved bioavailability of protein, amino acids, and dietary essential minerals from cowpea in growing rats, thus strengthening its potential value as functional food or food supplement. Forty Wistar albino rats (48 ± 1.8 g), were fed one of four experimental diets (n = 10 rats per diet): casein, raw cowpea, fermented cowpea or fermented and autoclaved cowpea. Despite lower growth indices of raw and fermented cowpea protein (PER, FTI) than casein, fermentation enhanced apparent digestibility of arginine, leucine, lysine, methionine, phenylalanine, tyrosine, and valine, and true digestibility of essential amino acids, except for tyrosine and valine, compared to raw cowpea. On the other hand, autoclaving of fermented cowpea flour decreased apparent, as did true digestibility of sulfur amino acids. Regarding the nutritive utilization of dietary essential minerals, Vigna unguiculata was a good source of available P, Mg, and K, while fermentation significantly improved the availability of P. Overall, cowpea was a good source of digestible essential amino acids and minerals and fermentation significantly improved its nutritional value that was not further enhanced by autoclaving.
Alcohol Consumption and the Risk of Prostate Cancer: A Dose-Response Meta-Analysis
Authors: Hong SungEun , Khil Hayeong , Lee Dong Hoon , Keum NaNa , Giovannucci Edward L.
Published: July 2020

Alcohol is widely consumed and is known as a major risk factor for several types of cancers. Yet, it is unclear whether alcohol consumption is associated with the risk of prostate cancer (PCa) or not. We conducted linear and non-linear dose–response meta-analyses of cohort studies on alcohol consumption and PCa risk by types of alcohol (total, wine, beer, and liquor) and PCa (non-aggressive and aggressive). Pubmed and Embase were searched through April 2020 to identify relevant studies. Summary relative risk (RR) and 95% confidence interval (CI) were estimated using a random-effects model. For non-aggressive PCa, by alcohol type, the risk increased linearly with liquor (RR per 14 g/day intake (alcohol content in standard drink) being 1.04 (95% CI = 1.02–1.06, I2 = 0%, three studies) and non-linearly with beer (Pnon-linearity = 0.045, four studies), with increased risk observed in the lower range (RR = 1.03, 95% CI = 1.01–1.05; 14 g/day), with 1.05 (95% CI = 1.01–1.08) at 28 g/day. Wine was not significantly associated with the risk of non-aggressive PCa. For aggressive PCa, a non-linear relationship of diverse shapes was indicated for all types of alcohol in the sensitivity analysis. Compared to non-drinking, a significant positive association was more apparent at lower dose for liquor (RR = 1.12, 95% CI = 1.04–1.20 at 14 g/day; RR = 1.16, 95% CI = 1.03–1.31 at 28 g/day; Pnon-linearity = 0.005, three studies) but at higher doses for wine (RR = 1.02, 95% CI = 0.90–1.16 at 28 g/day, RR = 1.35, 95% CI = 1.08–1.67 at 56 g/day; Pnon-linearity = 0.01, four studies). In contrast, decreased risks were indicated at lower doses of beer (RR = 0.85, 95% CI = 0.79–0.92 at 14 g/day; RR = 0.79, 95% CI = 0.70–0.90 at 28 g/day, Pnon-linearity < 0.001, four studies). Total alcohol consumption was not associated with both types of PCa. In this study, we found heterogeneous associations between alcohol intake and PCa by types of alcohol and PCa.
Gut Health-Promoting Benefits of a Dietary Supplement of Vitamins with Inulin and Acacia Fibers in Rats
Authors: Massot-Cladera Malén , Azagra-Boronat Ignasi , Franch Àngels , Castell Margarida , Rodríguez-Lagunas Maria J. , Pérez-Cano Francisco J.
Published: July 2020

The study’s objective was to ascertain whether a nutritional multivitamin and mineral supplement enriched with two different dietary fibers influences microbiota composition, mineral absorption, and some immune and metabolic biomarkers in adult rats. Nine-week-old Wistar rats were randomly assigned into four groups: the reference group; the group receiving a daily supplement based on a food matrix with proteins, vitamins, and minerals; and two other groups receiving this supplement enriched with inulin (V + I) or acacia (V + A) fiber for four weeks. Microbiota composition was determined in cecal content and mineral content in fecal, blood, and femur samples. Intestinal IgA concentration, hematological, and biochemical variables were evaluated. Both V + I and V + A supplementations increased Firmicutes and Actinobacteria phyla, which were associated with a higher presence of Lactobacillus and Bifidobacterium spp. V + A supplementation increased calcium, magnesium, phosphorus, and zinc concentrations in femur. V + I supplementation increased the fecal IgA content and reduced plasma total cholesterol and uric acid concentration. Both fiber-enriched supplements tested herein seem to be beneficial to gut-health, although differently.
Vitamin C for Cardiac Protection during Percutaneous Coronary Intervention: A Systematic Review of Randomized Controlled Trials
Authors: Khan Sher Ali , Bhattacharjee Sandipan , Ghani Muhammad Owais Abdul , Walden Rachel , Chen Qin M.
Published: July 2020

Percutaneous coronary intervention (PCI) is the preferred treatment for acute coronary syndrome (ACS) secondary to atherosclerotic coronary artery disease. This nonsurgical procedure is also used for selective patients with stable angina. Although the procedure is essential for restoring blood flow, reperfusion can increase oxidative stress as a side effect. We address whether intravenous infusion of vitamin C (VC) prior to PCI provides a benefit for cardioprotection. A total of eight randomized controlled trials (RCT) reported in the literature were selected from 371 publications through systematic literature searches in six electronic databases. The data of VC effect on cardiac injury biomarkers and cardiac function were extracted from these trials adding up to a total of 1185 patients. VC administration reduced cardiac injury as measured by troponin and CK-MB elevations, along with increased antioxidant reservoir, reduced reactive oxygen species (ROS) and decreased inflammatory markers. Improvement of the left ventricular ejection fraction (LVEF) and telediastolic left ventricular volume (TLVV) showed a trend but inconclusive association with VC. Intravenous infusion of VC before PCI may serve as an effective method for cardioprotection against reperfusion injury.
Phloroglucinol Mediated Plant Regeneration of Ornithogalum dubium as the Sole “Hormone-Like Supplement” in Plant Tissue Culture Long-Term Experiments
Authors: Petti Carloalberto
Published: July 2020

Tissue culture is an essential requirement in plant science to preserve genetic resources and to expand naturally occurring germplasm. A variety of naturally occurring and synthetic hormones are available to induce the processes of dedifferentiation and redifferentiation. Not all plant material is susceptible to tissue culture, and often complex media and hormone requirements are needed to achieve successful plant propagations. The availability of new hormones or chemicals acting as hormones are critical to the expansion of tissue culture potentials. Phloroglucinol has been shown to have certain hormone-like properties in a variety of studies. Ornithogalum dubium, an important geophyte species, was used to characterise the potential of phloroglucinol as the sole plant-like hormone in a tissue culture experiment. Tissue culture, plant regeneration, total phenolic and genetic variability were established by applying a variety of methods throughout long-term experiments. Phloroglucinol did induce callus formation and plant regeneration when used as the sole supplement in the media at a rate of 37%, thus demonstrating auxin/cytokines-like properties. Callus formation was of 3 types, friable and cellular, hard and compact, and a mixture of the two. The important finding was that direct somatogenesis did occur albeit more frequently on younger tissue, whereby rates of induction were up to 52%. It is concluded that phloroglucinol acts as a “hormone-like” molecule and can trigger direct embryogenesis without callus formation.
Shading Affects Yield, Elemental Composition and Antioxidants of Perennial Wall Rocket Crops Grown from Spring to Summer in Southern Italy
Authors: Caruso Gianluca , Formisano Luigi , Cozzolino Eugenio , Pannico Antonio , El-Nakhel Christophe , Rouphael Youssef , Tallarita Alessio , Cenvinzo Vincenzo , De Pascale Stefania
Published: July 2020

Shading nets have been increasingly drawing research interest, as they allow us to improve the environmental conditions for greenhouse-grown crops. The effects of two shading nets (50% and 79% shading degree), plus an unshaded control, on yield, mineral composition and antioxidants of perennial wall rocket (Diplotaxis tenuifolia L.-D.C.) grown under tunnels in southern Italy were determined. The shading application resulted in a yield decrease, compared to the unshaded control, except for the highest production under 50% shading in July. The highest yield was recorded in the April–May and May–June and the lowest in July. Similar trends were recorded for plant dry weight, leaf number per rosette and mean weight, but the latter showed the highest value under 79% light extinction in July. The rocket leaves were brighter in the summer cycles than in the spring ones. Leaf nitrate was highest in spring and under 79% shading. Potassium, phosphorus, calcium and magnesium showed the highest values in spring and in the unshaded control. The lipophilic antioxidant activity showed the highest values under the 79% shading net in the spring cropping seasons, whereas in July it did not significantly differ from 50% light extinction. The hydrophilic antioxidant activity always attained the highest values in the unshaded control. The unshaded leaves had the highest total phenol accumulation when grown in April–May and the lowest in July. The total ascorbic acid content was always highest in the unshaded control leaves compared to the shading treatments. Fifty percent crop shading is, therefore, an effective sustainable tool for increasing the yield of perennial wall rocket leaves in July, when the light intensity under the plastic tunnel exceeds the plant requirements, also resulting in a mineral composition that is not significantly different from that of the unshaded crops.
Preparation and Characterization of Tris(trimethylsiloxy)silyl Modified Polyurethane Acrylates and Their Application in Textile Treatment
Authors: Yu Xuecheng , Xiong Ying , Li Zhen , Tang Hongding
Published: July 2020

Three series of silicone modified polyurethane acrylate (SPUA) prepolymers were prepared from dicyclohexylmethane-4, 4′-diisocyanate (HMDI), PPG1000, triethylene glycol (TEG), 2-hydroxyethyl acrylate (HEA), and multi-hydroxyalkyl silicone (MI-III) with tris(trimethylsiloxy)silyl propyl side groups. Their structures were confirmed by 1H NMR, 13C NMR, and Fourier transformed infrared (FTIR) analysis, and SPUA films were obtained by UV curing. The properties of films were investigated by attenuated total reflection (ATR)-FTIR, scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS), water contact angle (WCA), thermogravimetric analysis (TGA), differential scanning calorimeter (DSC), water and hexane resistance, and tensile testing. The results showed that the structures and dosages of MI-III could influence the polymerization properties, surface properties, water and n-hexane resistance, and thermal and tensile properties of SPUA. For instance, the surface aggregation of tris(trimethylsiloxy)silyl propyl groups (even ~2.5 wt%) could endow SPUA films with less microphase separation, good hydrophobicity, lipophilicity, thermal stability, and mechanical properties. Interestingly, obvious regular winkles appeared on the surfaces of SPUAIII films, which are characterized by relatively high WCA values. However, relatively smooth were observed on the surfaces of SPUAIII films, which also exhibit lower water absorption ratio values. Furthermore, the ordinary cotton textiles would be transformed into hydrophobic and oleophilic textiles after treating with SPUA simply, and they were used in the oil/water separation study. Among them, consistent with water and hexane resistance analysis of SPUA films, SPUAII treated cotton textiles are characterized by relatively small liquid absorption capacity (LAC) values. Thus, phenyl groups and side-chain tris(trimethylsiloxy)silyl propyl groups are helpful to improve the hydrophobicity and lipophilicity of SPUA films. SPUAII-5 (even with 5 wt% MII) treated cotton textiles could efficiently separate the oil/water mixture, such as n-hexane, cyclohexane, or methylbenzene with water. Thus, this material has great potential in the application of hydrophobic treatment, oil/water separation, and industrial sewage emissions, among others.
Silver Nanoparticles for Water Pollution Monitoring and Treatments: Ecosafety Challenge and Cellulose-Based Hybrids Solution
Authors: Fiorati Andrea , Bellingeri Arianna , Punta Carlo , Corsi Ilaria , Venditti Iole
Published: July 2020

Silver nanoparticles (AgNPs) are widely used as engineered nanomaterials (ENMs) in many advanced nanotechnologies, due to their versatile, easy and cheap preparations combined with peculiar chemical-physical properties. Their increased production and integration in environmental applications including water treatment raise concerns for their impact on humans and the environment. An eco-design strategy that makes it possible to combine the best material performances with no risk for the natural ecosystems and living beings has been recently proposed. This review envisages potential hybrid solutions of AgNPs for water pollution monitoring and remediation to satisfy their successful, environmentally safe (ecosafe) application. Being extremely efficient in pollutants sensing and degradation, their ecosafe application can be achieved in combination with polymeric-based materials, especially with cellulose, by following an eco-design approach. In fact, (AgNPs)–cellulose hybrids have the double advantage of being easily produced using recycled material, with low costs and possible reuse, and of being ecosafe, if properly designed. An updated view of the use and prospects of these advanced hybrids AgNP-based materials is provided, which will surely speed their environmental application with consequent significant economic and environmental impact.
Systematic Investigation on the Structure-Property Relationship in Isotactic Polypropylene Films Processed via Cast Film Extrusion
Authors: Di Sacco Federico , Gahleitner Markus , Wang Jingbo , Portale Giuseppe
Published: July 2020

The effect of cast film extrusion processing conditions, such as the chill-roll temperature, temperature of the melt, and line speed, on the structure of different isotactic polypropylene homo- and random copolymers has been investigated by means of Small- and Wide-Angle X-ray Scattering (SAXS and WAXS) and correlated to stiffness and haze. Stiffness and transparency have been found to be strongly dependent on the temperature of the chill-roll. Interestingly, line speed has been found to affect the total crystallinity when the chill-roll temperature is increased, while an overall minor effect of the melt temperature was found for all cast films. The polymer characteristics, defined by the catalyst nature and comonomer content, affect the final material performance, with the single-site catalyzed grades performing better in both mechanics and optics. Haze levels were found to correlate with the mesophase content rather than to α-crystallinity and to be dependent on the domain size for all grades. The remarkably low haze levels reached by the single-site grade with higher isotacticity can arise from high nucleation rate and orientational effects, which ultimately yield smaller and smoother scattering domains.
Correlation between Tribological Properties and the Quantified Structural Changes of Lysozyme on Poly (2-hydroxyethyl methacrylate) Contact Lens
Authors: Chang You-Cheng , Su Chen-Ying , Chang Chia-Hua , Fang Hsu-Wei , Wei Yang
Published: July 2020

The ocular discomfort is the leading cause of contact lens wear discontinuation. Although the tear proteins as a lubricant might improve contact lens adaptation, some in vitro studies suggested that the amount of adsorbed proteins could not simply explain the lubricating performance of adsorbed proteins. The purpose of this study was to quantify the structural changes and corresponding ocular lubricating properties of adsorbed protein on a conventional contact lens material, poly (2-hydroxyethyl methacrylate) (pHEMA). The adsorption behaviors of lysozyme on pHEMA were determined by the combined effects of protein–surface and protein–protein interactions. Lysozyme, the most abundant protein in tear, was first adsorbed onto the pHEMA surface under widely varying protein solution concentrations to saturate the surface, with the areal density of the adsorbed protein presenting different protein–protein effects within the layer. These values were correlated with the measured secondary structures, and corresponding friction coefficient of the adsorbed and protein covered lens surface, respectively. The decreased friction coefficient value was an indicator of the lubricated surfaces with improved adaptation. Our results indicate that the protein–protein effects help stabilize the structure of adsorbed lysozyme on pHEMA with the raised friction coefficient measured critical for the innovation of contact lens material designs with improved adaptation.
Combining Fluorinated Polymers with Ag Nanoparticles as a Route to Enhance Optical Properties of Composite Materials
Authors: Satulu Veronica , Mitu Bogdana , Ion Valentin , Marascu Valentina , Matei Elena , Stancu Cristian , Dinescu Gheorghe
Published: July 2020

Polymer-based nanocomposites have recently received considerable attention due to their unique properties, which makes them feasible for applications in optics, sensors, energy, life sciences, etc. The present work focuses on the synthesis of nanocomposites consisting of a polytetrafluorethylene-like matrix in which metallic nano-silver are embedded, by using multiple magnetron plasmas. By successively exposing the substrate to separate RF magnetrons using as combination of target materials polytetrafluorethylene (PTFE) and silver, individual control of each deposition process is insured, allowing obtaining of structures in which silver nanoparticles are entrapped in-between two PTFE layers with given thicknesses. The topographical and morphological characteristics investigated by means of Scanning Electron Microscopy and Atomic Force Microscopy techniques evidenced the very presence of Ag nanoparticles with typical dimension 7 nm. The chemical composition at various depositing steps was evaluated through X-ray Photoelectron Spectroscopy. We show that the presence of the top PTFE layer prevents silver oxidation, while its thickness allows the tailoring of optical properties, as evidenced by spectroellipsometry. The appearance of chemical bonds between silver atoms and PTFE atoms at interfaces is observed, pointing out that despite of pure physical deposition processes, a chemical interaction between the polymeric matrix and metal is promoted by plasma.
Quantifying the Actual Impacts of Forest Cover Change on Surface Temperature in Guangdong, China
Authors: Shen Wenjuan , He Jiaying , Huang Chengquan , Li Mingshi
Published: July 2020

Forest cover change is critical in the regulation of global and regional climate change through the alteration of biophysical features across the Earth’s surface. The accurate assessment of forest cover change can improve our understanding of its roles in the regulation processes of surface temperature. In spite of this, few researchers have attempted to discern the varying effects of multiple satellite-derived forest changes on local surface temperatures. In this study, we quantified the actual contributions of forest loss and gain associated with evapotranspiration (ET) and albedo to local surface temperature in Guangdong Province, China using an improved spatiotemporal change pattern analysis method, and explored the interrelationships between surface temperature and air temperature change. We specifically developed three forest change products for Guangdong, combining satellite observations from Landsat, PALSAR, and MODIS for comparison. Our results revealed that the adjusted simple change detection (SCD)-based Landsat/PALSAR forest cover data performed relatively well. We found that forest loss and gain between 2000 and 2010 had opposite effects on land surface temperature (LST), ET, and albedo. Forest gain led to a cooling of −0.12 ± 0.01 °C, while forest loss led to a warming of 0.07 ± 0.01 °C, which were opposite to the anomalous change of air temperature. A reduced warming to a considerable cooling was estimated due to the forest gain and loss across latitudes. Specifically, mid-subtropical forest gains increased LST by 0.25 ± 0.01 °C, while tropical forest loss decreased LST by −0.16 ± 0.05 °C, which can demonstrate the local differences in an overall cooling. ET induced cooling and warming effects were appropriate for most forest gain and loss. Meanwhile, the nearby temperature changes caused by no-change land cover types more or less canceled out some of the warming and cooling. Albedo exhibited negligible and complex impacts. The other two products (i.e., the GlobeLand30 and MCD12Q1) affect the magnitude of temperature response due to the discrepancies in forest definition, methodology, and data resolution. This study highlights the non-negligible contributions of high-resolution maps and a robust temperature response model in the quantification of the extent to which forest gain reverses the climate effects of forest loss under global warming.
Machine Learning for Cloud Detection of Globally Distributed Sentinel-2 Images
Authors: Cilli Roberto , Monaco Alfonso , Amoroso Nicola , Tateo Andrea , Tangaro Sabina , Bellotti Roberto
Published: July 2020

In recent years, a number of different procedures have been proposed for segmentation of remote sensing images, basing on spectral information. Model-based and machine learning strategies have been investigated in several studies. This work presents a comprehensive overview and an unbiased comparison of the most adopted segmentation strategies: Support Vector Machines (SVM), Random Forests, Neural networks, Sen2Cor, FMask and MAJA. We used a training set for learning and two different independent sets for testing. The comparison accounted for 135 images acquired from 54 different worldwide sites. We observed that machine learning segmentations are extremely reliable when the training and test are homogeneous. SVM performed slightly better than other methods. In particular, when using heterogeneous test data, SVM remained the most accurate segmentation method while state-of-the-art model-based methods such as MAJA and FMask obtained better sensitivity and precision, respectively. Therefore, even if each method has its specific advantages and drawbacks, SVM resulted in a competitive option for remote sensing applications.
Orbital Lifetime (2008–2017) Radiometric Calibration and Evaluation of the HJ-1B IRS Thermal Infrared Band
Authors: Liu Wanyue , Li Jiaguo , Han Qijin , Zhu Li , Yang Hongyan , Cheng Qiuming
Published: July 2020

The infrared sensor (IRS) is a payload on the HJ-1B satellite and includes a thermal infrared band (B08). In order to obtain radiometric calibration coefficients and evaluate annual change, this study performed an analysis covering its 10-year orbital lifetime (2008–2017). The cross-calibration of IRS B08 with MODIS was performed using near-simultaneous images over Lake Qinghai, China. The results reveal that the radiometric response of IRS B08 notably changed during its orbital lifetime from year-to-year. The offsets fluctuated more than the gain. The top-of-atmosphere (TOA) radiance obtained by calibration coefficients in this study was generally in agreement with those obtained by onboard calibrator, within an error range of ±4.00% from 2008 to 2012. The percent difference compared with field validation was within 1.63%. The difference between IRS and MODIS radiance over field validation sites was within ±5%. Approximately a 1% difference occurred between the TOA temperature of IRS and MODIS. The radiometric response of IRS B08 continuously decreased from 2008 to 2013, whereas it fluctuated from 2014 to 2017. Moreover, the DN fluctuated more when the at-aperture radiance was low, although it was more stable at higher radiance.
Automated Algorithm for Removing Clutter Objects in MMS Point Cloud for 3D Road Mapping
Authors: Lee Jisang , Yoo Suhong , Hong Seunghwan , Farkoushi Mohammad Gholami , Bae Junsu , Park Ilsuk , Sohn Hong-Gyoo
Published: July 2020

Road information high definition maps (HD map) contain information about the facilities around the roads and are often constructed through a mobile mapping system (MMS). Although constructing an HD map is essential for road maintenance and the application of autonomous driving in the future, it is problematic to acquire the data of objects other than the facilities in an unstructured form while operating the MMS. In this study, the researchers define this object data as clutter objects and present a method of automatic removal using characteristics of the MMS and image segmentation techniques. By applying the method to 10 KITTI (Karlsruhe Institute of Technology and Toyota Technological Institute at Chicago) datasets, clutter objects were removed with an average overall accuracy of 91% with 0% (0.448%) error of commission for the complete point cloud map.