bluuebunny/crossref_metadata_2025 huggingface dataset:
Perceived Training of Junior Speed Skaters versus the Coach’s Intention: Does a Mismatch Relate to Perceived Stress and Recovery?
Authors: Otter Ruby T. A. , Bakker Anna C. , van der Zwaard Stephan , Toering Tynke , Goudsmit Jos F. A. , Stoter Inge K. , de Jong Johan
Published: September 2022

The aim of this observational study was to examine the differences between training variables as intended by coaches and perceived by junior speed skaters and to explore how these relate to changes in stress and recovery. During a 4-week preparatory period, intended and perceived training intensity (RPE) and duration (min) were monitored for 2 coaches and their 23 speed skaters, respectively. The training load was calculated by multiplying RPE by duration. Changes in perceived stress and recovery were measured using RESTQ-sport questionnaires before and after 4 weeks. Results included 438 intended training sessions and 378 executed sessions of 14 speed skaters. A moderately higher intended (52:37 h) versus perceived duration (45:16 h) was found, as skaters performed fewer training sessions than anticipated (four sessions). Perceived training load was lower than intended for speed skating sessions (−532 ± 545 AU) and strength sessions (−1276 ± 530 AU) due to lower RPE scores for skating (−0.6 ± 0.7) or shorter and fewer training sessions for strength (−04:13 ± 02:06 hh:mm). All training and RESTQ-sport parameters showed large inter-individual variations. Differences between intended–perceived training variables showed large positive correlations with changes in RESTQ-sport, i.e., for the subscale’s success (r = 0.568), physical recovery (r = 0.575), self-regulation (r = 0.598), and personal accomplishment (r = 0.589). To conclude, speed skaters that approach or exceed the coach’s intended training variables demonstrated an increased perception of success, physical recovery, self-regulation, and personal accomplishment.
Investigating the Effect of COVID-19 on Driver Behavior and Road Safety: A Naturalistic Driving Study in Malaysia
Authors: Al-Hussein Ward Ahmed , Li Wenshuang , Por Lip Yee , Ku Chin Soon , Alredany Wajdi Hamza Dawod , Leesri Thanakamon , MohamadJawad Huda Hussein
Published: September 2022

The spread of the novel coronavirus COVID-19 resulted in unprecedented worldwide countermeasures such as lockdowns and suspensions of all retail, recreational, and religious activities for the majority of 2020. Nonetheless, no adequate scientific data have been provided thus far about the impact of COVID-19 on driving behavior and road safety, especially in Malaysia. This study examined the effect of COVID-19 on driving behavior using naturalistic driving data. This was accomplished by comparing the driving behaviors of the same drivers in three periods: before COVID-19 lockdown, during COVID-19 lockdown, and after COVID-19 lockdown. Thirty people were previously recruited in 2019 to drive an instrumental vehicle on a 25 km route while recording their driving data such as speed, acceleration, deceleration, distance to vehicle ahead, and steering. The data acquisition system incorporated various sensors such as an OBDII reader, a lidar, two ultrasonic sensors, an IMU, and a GPS. The same individuals were contacted again in 2020 to drive the same vehicle on the same route in order to capture their driving behavior during the COVID-19 lockdown. Participants were approached once again in 2022 to repeat the procedure in order to capture their driving behavior after the COVID-19 lockdown. Such valuable and trustworthy data enable the assessment of changes in driving behavior throughout the three time periods. Results showed that drivers committed more violations during the COVID-19 lockdown, with young drivers in particular being most affected by the traffic restrictions, driving significantly faster and performing more aggressive steering behaviors during the COVID-19 lockdown than any other time. Furthermore, the locations where the most speeding offenses were committed are highlighted in order to provide lawmakers with guidance on how to improve traffic safety in those areas, in addition to various recommendations on how to manage traffic during future lockdowns.
Preventing Drift through Continued Co-Design with a First Nations Community: Refining the Prototype of a Tiered FASD Assessment
Authors: Miller Luke , Shanley Dianne C. , Page Marjad , Webster Heidi , Liu Wei , Reid Natasha , Shelton Doug , West Karen , Marshall Joan , Hawkins Erinn
Published: September 2022

As part of the broader Yapatjarrathati project, 47 remote health providers and community members attended a two-day workshop presenting a prototype of a culturally-safe, tiered neurodevelopmental assessment that can identify fetal alcohol spectrum disorder (FASD) in primary healthcare. The workshop provided a forum for broad community feedback on the tiered assessment process, which was initially co-designed with a smaller number of key First Nations community stakeholders. Improvement in self-reported attendee knowledge, confidence, and perceived competence in the neurodevelopmental assessment process was found post-workshop, assessed through self-report questionnaires. Narrative analysis described attendee experiences and learnings (extracted from the workshop transcript), and workshop facilitator experiences and learnings (extracted from self-reflections). Narrative analysis of the workshop transcript highlighted a collective sense of compassion for those who use alcohol to cope with intergenerational trauma, but exhaustion at the cyclical nature of FASD. There was a strong desire for a shared responsibility for First Nations children and families and a more prominent role for Aboriginal Health Workers in the assessment process. Narrative analysis from workshop facilitator reflections highlighted learnings about community expertise, the inadvertent application of dominant cultural approaches throughout facilitation, and that greater emphasis on the First Nation’s worldview and connection to the community was important for the assessment process to be maintained long-term. This study emphasised the benefit of continued co-design to ensure health implementation strategies match the needs of the community.
Research on Selected Wildlife Infections in the Circumpolar Arctic—A Bibliometric Review
Authors: Emelyanova Anastasia , Savolainen Audrey , Oksanen Antti , Nieminen Pentti , Loginova Olga , Abass Khaled , Rautio Arja
Published: September 2022

One Health, a multidisciplinary approach to public health, which integrates human, animal, and environmental studies, is prudent for circumpolar Arctic health research. The objective of our bibliometric review was to identify and compare research in select infectious diseases in Arctic wildlife species with importance to human health indexed in English language databases (PubMed, Scopus) and the Russian database eLibrary.ru. Included articles (in English and Russian languages) needed to meet the following criteria: (1) data comes from the Arctic, (2) articles report original research or surveillance reports, (3) articles were published between 1990 and 2018, and (4) research relates to naturally occurring infections. Of the included articles (total n = 352), most were from Russia (n = 131, 37%), Norway (n = 58, 16%), Canada (n = 39, 11%), and Alaska (n = 39, 11%). Frequently reported infectious agents among selected mammals were Trichinella spp. (n = 39), Brucella spp. (n = 25), rabies virus (n = 11), Echinococcus spp. (n = 10), and Francisella tularensis (n = 9). There were 25 articles on anthrax in eLibrary.ru, while there were none in the other two databases. We identified future directions where opportunities for further research, collaboration, systematic reviews, or monitoring programs are possible and needed.
COVID-19 Case Tracking System in Quarantine Policy: Focus on the Privacy Shift Concept and Application in South Korea
Authors: Moon Kwansik , Kim Nackhwan , Lee Jemin Justin , Yoon Hyunsik , Lee Kyungho
Published: September 2022

This study analyzed how Korea’s quarantine policy manages personal information to prevent and control COVID-19. Korea effectively halted the spread of COVID-19 through epidemiological investigations and cell-broadcast systems. In this process, the route of infection is presented without identifying the patient, and the necessary participants are selected only through authentication. We found a correlation between the number of emergency text messages sent by the Ministry of Interior and Safety in 2020 and the number of confirmed cases (R2 = 0.465, p < 0.001). Based on Korea’s case, we propose a new concept for solving the personal information problems that might arise during a pandemic response.
Harmonization of Epidemiologic Research Methods to Address the Environmental and Social Determinants of Urban Slum Health Challenges in Sub-Saharan Africa
Authors: Mustapha Adetoun , Amegah A. Kofi , Coker Eric Stephen
Published: September 2022

Sub-Saharan Africa (SSA) has a significant proportion of populations living in urban slum conditions, where exposure to multiple environmental stressors and social inequalities is ubiquitous. This commentary synthesizes commonalities in recent environmental health studies from urban cities in East and West Africa, presented during a symposium sponsored by the Africa Chapter of the International Society of Environmental Epidemiology (ISEE) in August 2020. A key takeaway from this symposium is the need for harmonization of epidemiologic and exposure data collection in three domains tailored to the SSA context: (1) improvements in socioeconomic status (SES) measurement through harmonization in the conceptualization and operationalization of SES indicators; (2) improvements in air pollution exposure assessment in resource-constrained contexts by better integration, validation, and harmonization of exposure data of air pollution and mitigating factors; and (3) harmonization in the assessment of health outcomes and biomonitoring of contaminants. Focusing on these three domains would galvanize environmental epidemiologists in SSA around shared data collection instruments and shared data platforms and facilitate the pooling of data across the continent. Fostering this collaborative research will enable researchers and decision-makers to glean new insights and develop robust environmental health interventions and policies for SSA urban slums and for improved population health.
Investigation of the Thermal Comfort Properties of Masks Used during the COVID-19 Pandemic
Authors: Oner Eren , Seçkin Ahmet Çağdaş , Egeli Dilara , Seçkin Mine
Published: September 2022

SARS-CoV-2, the causative agent of COVID-19, which was officially declared a pandemic by the World Health Organization (WHO) on 11 March 2020, is transmitted from person to person through respiratory droplets and close contact and can cause severe respiratory failure and pneumonia. Currently, while the worldwide COVID-19 pandemic is still ongoing and countries are taking strict precautions to protect populations against infection, the most effective precautions still seem to be social distancing and wearing a mask. The question of how effective masks were in the early stages of the COVID-19 pandemic has been widely discussed, both in public and scientific circles, and the protection of different mask types has been examined. This study aimed to examine the comfort conditions provided by the different mask types to the user during use. For this purpose, single-ply, double-ply, three-ply, cloth, FFP1, FFP2, and FFP3 masks with different standards were examined, with and without a valve. To conduct the experiments, the novel thermal head measurement system, developed within the scope of this study, was used specifically for mask comfort studies. Thanks to the developed measurement system, the thermal resistance and water vapor resistance values of different masks were measured, and their comfort conditions were evaluated. According to the findings, cloth masks provide a comfortable condition, with lower thermal resistance and water vapor resistance values than other masks. In addition, it was observed that surgical masks offer better thermal comfort conditions, although they have lower protection than FFP masks.
An Innovative Tool to Control Occupational Radon Exposure
Authors: Martin-Gisbert Lucía , Ruano-Ravina Alberto , Barros-Dios Juan Miguel , Varela-Lema Leonor , Pérez-Ríos Mónica
Published: September 2022

After smoking, indoor radon is the main contributor to lung cancer in many countries. The European Union (EU) Directive 2013/59/Euratom establishes a maximum reference level of 300 Bq/m3 of radon concentration in the workplace, and an effective dose limit of 20 mSv per year for workers. If the radon concentration in a workplace exceeds the reference level, constructive mitigation applies. When constructive mitigation is not feasible, we propose to keep workers’ effective dose below 6 mSv per year (category B of exposed workers) by controlling occupancy time. Setting the maximum annual dose at 6 mSv protects workers’ health and eases the regulatory requirements for employers. If multisite workers are present, each worker has to be monitored individually by tracking the time spent and the radon concentration at each worksite. This paper shows a software tool for employers to perform this complex tracking in an accurate, conservative, and transparent manner, and in compliance with the EU by-laws.
Attempting to Increase the Effectiveness of the Antidepressant Trazodone Hydrochloride Drug Using π-Acceptors
Authors: Alsanie Walaa F. , Alhomrani Majid , Alamri Abdulhakeem S. , Alyami Hussain , Shakya Sonam , Habeeballah Hamza , Alkhatabi Heba A. , Felimban Raed I. , Alamri Abdulwahab , Alhabeeb Abdulhameed Abdullah , Raafat Bassem M. , Refat Moamen S. , Gaber Ahmed
Published: September 2022

Major depressive disorder is a prevalent mood illness that is mildly heritable. Cases with the highest familial risk had recurrence and onset at a young age. Trazodone hydrochloride is an antidepressant medicine that affects the chemical messengers in the brain known as neurotransmitters, which include acetylcholine, norepinephrine, dopamine, and serotonin. In the present research, in solid and liquid phases, the 1:1 charge-transfer complexes between trazodone hydrochloride (TZD) and six different π-acceptors were synthesized and investigated using different microscopic techniques. The relation of dative ion pairs [TZD+, A−], where A is the acceptor, was inferred via intermolecular charge-transfer complexes. Additionally, a molecular docking examination was utilized to compare the interactions of protein receptors (serotonin-6BQH) with the TZD alone or in combination with the six distinct acceptor charge-transfer complexes. To refine the docking results acquired from AutoDock Vina and to better examine the molecular mechanisms of receptor-ligand interactions, a 100 ns run of molecular dynamics simulation was used. All the results obtained in this study prove that the 2,6-dichloroquinone-4-chloroimide (DCQ)/TZD complex interacts with serotonin receptors more efficiently than reactant donor TZD only and that [(TZD)(DCQ)]-serotonin has the highest binding energy value of all π-acceptor complexes.
Exploring the Relationships between Children’s Oral Health and Parents’ Oral Health Knowledge, Literacy, Behaviours and Adherence to Recommendations: A Cross-Sectional Survey
Authors: Chawłowska Ewelina , Karasiewicz Monika , Lipiak Agnieszka , Cofta Mateusz , Fechner Brittany , Lewicka-Rabska Agnieszka , Pruciak Agata , Gerreth Karolina
Published: September 2022

Although preventable, early childhood caries (ECC) is a burdening condition in all WHO regions, with Poland being one of the most affected countries in Europe. Effective home-based prevention of ECC is available and recommended by several expert bodies. Therefore, we wanted to determine how well parents were following selected oral health recommendations in preschool children. Additionally, we wanted to determine which socioeconomic factors influenced such practices the most, and how parents’ oral health literacy and behaviours affected the oral hygiene and oral health status of their children. A cross-sectional survey involving 2338 parents and caregivers of children from 167 kindergartens was performed. Children’s oral hygiene and oral health status were found to be associated with parents’ education and economic status. They were also strongly influenced by parental health literacy and behaviours. With respect to recommendations for preschoolers, the lowest adherence was found in the use of fluoride toothpaste and its age-appropriate amount, supervision of toothbrushing, and refraining from eating after last toothbrushing. The improvement in these areas could be achieved thanks to the involvement of health professionals such as gynaecologists, midwives, and primary care personnel in efforts aimed at increasing parents’ oral health knowledge and literacy.
Moderate and Severe Dental Fluorosis in the Rural Population of Anantapur, India: Change in Their Biological Susceptibility?
Authors: García-Escobar Trina Mylena , Valdivia-Gandur Iván , Astudillo-Rozas Wilson , Aceituno-Antezana Oscar , Yamadala Balasubbaiah , Lozano de Luaces Vicente , Chimenos-Küstner Eduardo , Manzanares-Céspedes María Cristina
Published: September 2022

Dental fluorosis affects the quality of life. A cross-sectional, observational study was conducted in a community affected by endemic fluorosis for several generations with a conserved biological and social environment. The study included patients from the rural population of Anantapur, India. The Dean index (DI) and the Thylstrup and Fejerskov Index (TFI) were used for fluorosis classification. Additionally, water samples were collected for fluoride analysis, taken from the patients’ living areas. The statistical association between the variables was analyzed. In total, 785 patients between 10 and 60 years old were included in the study (58.7% women and 41.3% men). Fluorosis signs were found in 94.6% of patients examined using the DI and 94.4% using the TFI. Moderate–severe dental fluorosis was observed in 62.8% by DI and 73.1% by TFI consuming untreated water with up to 2.9 ppm of fluoride. Furthermore, moderate–severe dental fluorosis was observed in 33.2% by DI and 39.9% by TFI consuming water with ≤1.5 ppm of fluoride. The high prevalence of moderate–severe dental fluorosis in patients consuming water with a low fluoride concentration suggests that other factors are involved. Biological susceptibility change could play an essential role in the severity of dental fluorosis in populations exposed for several generations, affecting its actual and future quality of life.
Evolution of the Knowledge Mapping of Climate Change Communication Research: Basic Status, Research Hotspots, and Prospects
Authors: Wu Meifen , Long Ruyin , Yang Shuhan , Wang Xinru , Chen Hong
Published: September 2022

Climate change communication is a crucial strategy for addressing the major challenges of climate change, and the knowledge mapping analysis and overview of it helps to clarify research progress. Based on CiteSpace, 428 pieces of domestic and foreign literature are collected to clarify the basic status of climate change communication research and summarize research hotspots and prospects. The study found that: (1) The earliest traceable English literature on climate change communication appeared in 2000. The number of articles published has risen steadily since 2008, reaching its first peak in 2015. (2) In contrast, research into Chinese climate change communication began late and progressed slowly. The Chinese literature on climate change communication first appeared in 2009. Although domestic research generally continues to pay attention to this phenomenon, there is still room for development compared with international research. (3) The research hotspots for climate change communication are obtained through keyword co-occurrence analysis. Public perceptions of climate change are strongly influenced by political ideology. Since climate change has political attributes, people from different political parties or groups form their views on climate change through identity protection. (4) The research content on climate change communication can be summarized into the following six aspects: the development of climate change communication research; differences in public perceptions of climate change; factors influencing climate change communication; key elements of the climate change communication process; the important role of the media in climate change communication; and effective strategies for climate change communication. Finally, the shortcomings of this study are summarized and future research prospects on climate change communication are put forward from the perspectives of research methods, research contexts, and research paradigms.
Digital Help for Substance Users (SU): A Systematic Review
Authors: Bonfiglio Natale Salvatore , Mascia Maria Lidia , Cataudella Stefania , Penna Maria Pietronilla
Published: September 2022

The estimated number of Substance Users (SU) globally has currently reached a very high number and is still increasing. This aspect necessitates appropriate interventions for prevention and specific treatments. The literature shows that digital treatments can be useful in the context of health services and substance abuse. This systematic review focuses mainly on research on the effectiveness of digital treatments for SU. Data sources included studies found on PsycINFO, PubMed, SCOPUS, and WebOfScience (WOS) database searches. The following keywords were used: TITLE (digital OR computer OR software OR tablet OR app OR videogame OR seriousgame OR virtualreality) AND ABSTRACT((mental AND health) AND (addiction OR dependence OR substance OR drug)). We focused on peer-reviewed articles published from 2010 through 2021 using PRISMA guidelines. A total of 18 studies met the inclusion criteria (i.e., type of intervention, efficacy in terms of misuse of substances and scored outcomes from questionnaire or toxicology tests, study methodology). The studies included investigations of specific digital treatments for SU of various kinds of drugs. The interventions were administered using personal computers, smartphones, or, in a few cases, tablets. Most of the interventions focused on the cognitive behavior therapy (CBT) model and/or on the use strategies, tips, or feedback. A minority provided information or training programs. The current review shows that digital treatments and interventions are effective in reducing the frequency of use, augmenting abstinence, or reducing the gravity of dependence for most of the studies at post-treatment. However, due to the heterogeneity of the variables (i.e., substance type, digital tool used, and treatment administered), there was a reduced generalizability of the results. This review highlights the need to continue the research in this field, and above all, to create effective digital protocols.
Proposal of a Multi-Criteria Model for the Evaluation of Territorial Development Plans: An Application in Chile’s Lagging Areas
Authors: Arancibia-Carvajal Sara , Petit-Laurent Felipe , Troncoso María Paz , Vargas-Vargas Manuel
Published: September 2022

The evaluation of Territorial Development Plans (TDP) is a challenge most Latin American countries face. The problem arises in establishing a model to evaluate TDP that meets the criteria and indicators established in a national policy or regulatory framework under local needs. This study proposes an application to evaluate the TDP of the lagging areas in Chile based on the AHP multi-criteria methodology. This methodology allows to objectively unify the evaluation of the different plans, combining the different dimensions, objectives, scales, and judgments of the experts present in the evaluation process without sacrificing the quality, reliability, and participation of the actors involved. The model is flexible to changes in the criteria, as it can be updated according to the needs over time. An efficient and effective tool is provided to support decision-makers in formulating better development plans to bridge the gaps in territorial groups with high vulnerability.
Recurring Rural Destination Sport Events: A Study on Participants’ Direct Spending
Authors: Tzoumaka Eugenia , Leivadi Stella , Kaplanidou Kyriaki (Kiki)
Published: September 2022

The current study explores direct spending profiles of sport event tourists in a very small rural destination as an alternative to the multiplier effect economic impact studies. Sport event tourism has been used as an economic engine tool by a variety of destination sizes but has been neglected for small rural destinations, where people arrive from various distance radiuses to participate in this central activity for the place. Data were collected from participants at a small-scale recurring mountain running event. An online survey instrument was sent to the participants after the event. Participants reported on their daily and total expenditures regarding accommodation, meals and other tourist spending. The results revealed that the participants who responded to the survey spent on average about EUR 163 for accommodation, EUR 205 for meals and about EUR 38 for other tourist spending, such as souvenirs. Respondents from closer radius, spent less on average, approximately EUR 156, EUR 383 and EUR 26 for the respective spending categories. Rural destinations counting on hosting sport events for economic boosts in poorly economic areas witness considerable financial gains for the regions via the estimation of the more feasible direct spending calculations.
Elevated IL-6 and IL-22 in Early Pregnancy Are Associated with Worse Disease Course in Women with Inflammatory Bowel Disease
Authors: Wu Richard Y. , Xiao Karren , Hotte Naomi , Tandon Parul , Elloumi Yesmine , Ambrosio Lindsy , Dunsmore Garett , Elahi Shokrollah , Kroeker Karen I. , Dieleman Levinus A. , Madsen Karen L. , Huang Vivian
Published: September 2022

Inflammatory bowel diseases (IBD), including Ulcerative Colitis (UC) and Crohn’s disease (CD), are inflammatory conditions of the intestinal tract that affect women in their reproductive years. Pregnancy affects Th1- and Th2-cytokines, but how these changes occur during pregnancy in IBD is unclear. We performed a longitudinal profiling of serum cytokines in a cohort of 11 healthy pregnant women and 76 pregnant women with IBD from the first trimester of pregnancy to the first 12 months post-partum. Participants were monitored for biochemical disease activity (C-reactive protein [CRP] and fecal calprotectin [FCP]) and clinical activities. Maternal cytokines were measured using ELISA. We identified changes in Th1 and Th17 cytokines throughout pregnancy in healthy pregnant women. During pregnancy, maternal serum cytokine expressions were influenced by IBD, disease activity, and medications. Active UC was associated with an elevation in IL-21, whereas active CD was associated with elevated IFN-γ, IL-6, and IL-21. Interestingly, T1 serum cytokine levels of IL-22 (>0.624 pg/mL) and IL-6 (>0.648 pg/mL) were associated with worse IBD disease activity throughout pregnancy in women with UC and CD, respectively. This shows serum cytokines in pregnancy differ by IBD, disease activity, and medications. We show for the first time that T1 IL-22 and IL-6 correlate with IBD disease course throughout pregnancy.
Characterization of Recombinant Antimicrobial Peptide BMGlv2 Heterologously Expressed in Trichoderma reesei
Authors: Liang Qingping , Cao Linyuan , Zhu Changliang , Kong Qing , Sun Han , Zhang Fang , Mou Haijin , Liu Zhemin
Published: September 2022

Antimicrobial peptides (AMPs) serve as alternative candidates for antibiotics and have attracted the attention of a wide range of industries for various purposes, including the prevention and treatment of piglet diarrhea in the swine industry. Escherichia coli, Salmonella, and Clostridium perfringens are the most common pathogens causing piglet diarrhea. In this study, the antimicrobial peptide gloverin2 (BMGlv2), derived from Bombyx mandarina, was explored to determine the efficient prevention effect on bacterial piglet diarrhea. BMGlv2 was heterologously expressed in Trichoderma reesei Tu6, and its antimicrobial properties against the three bacteria were characterized. The results showed that the minimum inhibitory concentrations of the peptide against E. coli ATCC 25922, S. derby ATCC 13076, and C. perfringens CVCC 2032 were 43.75, 43.75, and 21.86 μg/mL, respectively. The antimicrobial activity of BMGlv2 was not severely affected by high temperature, salt ions, and digestive enzymes. It had low hemolytic activity against rabbit red blood cells, indicating its safety for use as a feed additive. Furthermore, the measurements of the leakage of bacterial cell contents and scanning electron microscopy of C. perfringens CVCC 2032 indicated that BMGlv2 exerted antimicrobial activity by destroying the cell membrane. Overall, this study showed the heterologous expression of the antimicrobial peptide BMGlv2 in T. reesei and verified its antimicrobial properties against three common pathogenic bacteria associated with piglet diarrhea, which can provide a reference for the applications of AMPs as an alternative product in industrial agriculture.
Xenobiotics Delivered by Electronic Nicotine Delivery Systems: Potential Cellular and Molecular Mechanisms on the Pathogenesis of Chronic Kidney Disease
Authors: Scharf Pablo , Rizzetto Felipe , Xavier Luana Filippi , Farsky Sandra Helena Poliselli
Published: September 2022

Chronic kidney disease (CKD) is characterized as sustained damage to the renal parenchyma, leading to impaired renal functions and gradually progressing to end-stage renal disease (ESRD). Diabetes mellitus (DM) and arterial hypertension (AH) are underlying diseases of CKD. Genetic background, lifestyle, and xenobiotic exposures can favor CKD onset and trigger its underlying diseases. Cigarette smoking (CS) is a known modified risk factor for CKD. Compounds from tobacco combustion act through multi-mediated mechanisms that impair renal function. Electronic nicotine delivery systems (ENDS) consumption, such as e-cigarettes and heated tobacco devices, is growing worldwide. ENDS release mainly nicotine, humectants, and flavorings, which generate several byproducts when heated, including volatile organic compounds and ultrafine particles. The toxicity assessment of these products is emerging in human and experimental studies, but data are yet incipient to achieve truthful conclusions about their safety. To build up the knowledge about the effect of currently employed ENDS on the pathogenesis of CKD, cellular and molecular mechanisms of ENDS xenobiotic on DM, AH, and kidney functions were reviewed. Unraveling the toxic mechanisms of action and endpoints of ENDS exposures will contribute to the risk assessment and implementation of proper health and regulatory interventions.
Histamine Activates Human Eosinophils via H2R and H4R Predominantly in Atopic Dermatitis Patients
Authors: Beyer Leonie , Kabatas Aylin Sara , Mommert Susanne , Stark Holger , Werfel Thomas , Gutzmer Ralf , Schaper-Gerhardt Katrin
Published: September 2022

Atopic dermatitis (AD) is maintained by a variety of cells and inflammatory mediators, including eosinophils and histamine. We recently reported that eosinophils from AD patients highly express the H4R. However, its immunomodulatory function in eosinophils is still largely unexplored. In this study, transcriptome analysis of blood eosinophils from AD patients stimulated with histamine and the H4R agonist ST-1006 revealed several regulated genes (e.g., IL-18R, IL-1RL1, PDE4B, CXCR4) involved in inflammation. Subsequently, the impact of histamine on one of the strongly regulated genes, the IL-18 receptor (IL-18Rα), was investigated in detail. Stimulation with histamine induced the upregulation of IL-18Rα at mRNA and at the protein level in human eosinophils, which was more pronounced in cells from AD patients than in cells from healthy controls. IL-18 was upregulated via histamine as well. After pre-incubation with histamine and IFN-γ, subsequent stimulation with IL-18 resulted in an increased ECP mRNA expression. The activation of eosinophils by histamine, in combination with IFN-γ and IL-5, was also accompanied by an upregulation of CD69. Thus, our results indicate a crucial role of histamine in the upregulation of the IL-18/IL-18R axis and in the activation of human eosinophils from AD patients.
Th17, Th22, and Myeloid-Derived Suppressor Cell Population Dynamics and Response to IL-6 in 4T1 Mammary Carcinoma
Authors: Rasé Viva J. , Hayward Reid , Haughian James M. , Pullen Nicholas A.
Published: September 2022

Immunotherapies relying on type 1 immunity have shown robust clinical responses in some cancers yet remain relatively ineffective in solid breast tumors. Polarization toward type 2 immunity and expansion of myeloid-derived suppressor cells (MDSC) confer resistance to therapy, though it remains unclear whether polarization toward type 3 immunity occurs or has a similar effect. Therefore, we investigated the involvement of type 3 Th17 and Th22 cells and their association with expanding MDSC populations in the 4T1 mouse mammary carcinoma model. Th17 and Th22 were detected in the earliest measurable mass at d 14 and remained present until the final sampling on d 28. In peripheral organs, Th17 populations were significantly higher than the non-tumor bearing control and peaked early at d 7, before a palpable tumor had formed. Peripheral Th22 proportions were also significantly increased, though at later times when tumors were established. To further address the mechanism underlying type 3 immune cell and MDSC recruitment, we used CRISPR-Cas9 to knock out 4T1 tumor production of interleukin-6 (4T1-IL-6-KO), which functions in myelopoiesis, MDSC recruitment, and Th maturation. While 4T1-IL-6-KO tumor growth was similar to the control, the reduced IL-6 significantly expanded the total CD4+ Th population and Th17 in tumors, while Th22 and MDSC were reduced in all tissues; this suggests that clinical IL-6 depletion combined with immunotherapy could improve outcomes. In sum, 4T1 mammary carcinomas secrete IL-6 and other factors, to polarize and reshape Th populations and expand distinct Th17 and Th22 populations, which may facilitate tumor growth and confer immunotherapy resistance.
Modulation of Specific Sphingosine-1-Phosphate Receptors Augments a Repair Mediating Schwann Cell Phenotype
Authors: Schira-Heinen Jessica , Wang Luzhou , Akgün Seda , Blum Sofia , Ziegler Brigida , Heinen André , Hartung Hans-Peter , Küry Patrick
Published: September 2022

Transdifferentiation of Schwann cells is essential for functional peripheral nerve regeneration after injury. By activating a repair program, Schwann cells promote functional axonal regeneration and remyelination. However, chronic denervation, aging, metabolic diseases, or chronic inflammatory processes reduce the transdifferentiation capacity and thus diminish peripheral nerve repair. It was recently described that the sphingosine-1-phosphate receptor (S1PR) agonist Fingolimod enhances the Schwann cell repair phenotype by activation of dedifferentiation markers and concomitant release of trophic factors resulting in enhanced neurite growth. Since Fingolimod targets four out of five S1PRs (S1P1, S1P3-5) possibly leading to non-specific adverse effects, identification of the main receptor(s) responsible for the observed phenotypic changes is mandatory for future specific treatment approaches. Our experiments revealed that S1P3 dominates and that along with S1P1 acts as the responsible receptor for Schwann cell transdifferentiation as revealed by the combinatory application of specific agonists and antagonists. Targeting both receptors reduced the expression of myelin-associated genes, increased PDGF-BB representing enhanced trophic factor expression likely to result from c-Jun induction. Furthermore, we demonstrated that S1P4 and S1P5 play only a minor role in the adaptation of the repair phenotype. In conclusion, modulation of S1P1 and S1P3 could be effective to enhance the Schwann cell repair phenotype and thus stimulate proper nerve repair.
Adjusting Some Properties of Poly(methacrylic acid) (Nano)Composite Hydrogels by Means of Silicon-Containing Inorganic Fillers
Authors: Ninciuleanu Claudia Mihaela , Ianchiș Raluca , Alexandrescu Elvira , Mihăescu Cătălin Ionuț , Burlacu Sabina , Trică Bogdan , Nistor Cristina Lavinia , Preda Silviu , Scomoroscenco Cristina , Gîfu Cătălina , Petcu Cristian , Teodorescu Mircea
Published: September 2022

The present work aims to show how the main properties of poly(methacrylic acid) (PMAA) hydrogels can be engineered by means of several silicon-based fillers (Laponite XLS/XLG, montmorillonite (Mt), pyrogenic silica (PS)) employed at 10 wt% concentration based on MAA. Various techniques (FT-IR, XRD, TGA, SEM, TEM, DLS, rheological measurements, UV-VIS) were used to comparatively study the effect of these fillers, in correlation with their characteristics, upon the structure and swelling, viscoelastic, and water decontamination properties of (nano)composite hydrogels. The experiments demonstrated that the nanocomposite hydrogel morphology was dictated by the way the filler particles dispersed in water. The equilibrium swelling degree (SDe) depended on both the pH of the environment and the filler nature. At pH 1.2, a slight crosslinking effect of the fillers was evidenced, increasing in the order Mt < Laponite < PS. At pH > pKaMAA (pH 5.4; 7.4; 9.5), the Laponite/Mt-containing hydrogels displayed a higher SDe as compared to the neat one, while at pH 7.4/9.5 the PS-filled hydrogels surprisingly displayed the highest SDe. Rheological measurements on as-prepared hydrogels showed that the filler addition improved the mechanical properties. After equilibrium swelling at pH 5.4, G’ and G” depended on the filler, the Laponite-reinforced hydrogels proving to be the strongest. The (nano)composite hydrogels synthesized displayed filler-dependent absorption properties of two cationic dyes used as model water pollutants, Laponite XLS-reinforced hydrogel demonstrating both the highest absorption rate and absorption capacity. Besides wastewater purification, the (nano)composite hydrogels described here may also find applications in the pharmaceutical field as devices for the controlled release of drugs.
Cell Contact with Endothelial Cells Favors the In Vitro Maintenance of Human Chronic Myeloid Leukemia Stem and Progenitor Cells
Authors: Torres-Barrera Patricia , Moreno-Lorenzana Dafne , Alvarado-Moreno José Antonio , García-Ruiz Elena , Lagunas Cesar , Mayani Hector , Chávez-González Antonieta
Published: September 2022

Chronic Myeloid Leukemia (CML) originates in a leukemic stem cell that resides in the bone marrow microenvironment, where they coexist with cellular and non-cellular elements. The vascular microenvironment has been identified as an important element in CML development since an increase in the vascularization has been suggested to be related with poor prognosis; also, using murine models, it has been reported that bone marrow endothelium can regulate the quiescence and proliferation of leukemic stem and progenitor cells. This observation, however, has not been evaluated in primary human cells. In this report, we used a co-culture of primitive (progenitor and stem) CML cells with endothelial colony forming cells (ECFC) as an in vitro model to evaluate the effects of the vascular microenvironment in the leukemic hematopoiesis. Our results show that this interaction allows the in vitro maintenance of primitive CML cells through an inflammatory microenvironment able to regulate the proliferation of progenitor cells and the permanence in a quiescent state of leukemic stem cells.
Could BMPs Therapy Be Improved if BMPs Were Used in Composition Acting during Bone Formation in Endochondral Ossification?
Authors: Hyc Anna , Osiecka-Iwan Anna , Moskalewski Stanislaw
Published: September 2022

The discovery of bone morphogenetic proteins (BMPs) inspired hope for the successful treatment of bone disorders, but side effects worsening the clinical effects were eventually observed. BMPs exert a synergistic effect, stimulating osteogenesis; however, predicting the best composition of growth factors for use in humans is difficult. Chondrocytes present within the growth plate produce growth factors stored in calcified cartilage adhering to metaphysis. These factors stimulate initial bone formation in metaphysis. We have previously determined the growth factors present in bovine calcified cartilage and produced by rat epiphyseal chondrocytes. The results suggest that growth factors stimulating physiological ossification are species dependent. The collection of human calcified cartilage for growth factors determination does not appear feasible, but chondrocytes for mRNA determination could be obtained. Their collection from young recipients, in view of the Academy of Medical Royal Colleges Recommendation, would be ethical. The authors of this review do not have facilities to conduct such a study and can only appeal to competent institutions to undertake the task. The results could help to formulate a better recipe for the stimulation of bone formation and improve clinical results.
Effect of Mono- and Multichlorinated Organic Compounds—Chlorocyclohexane and Hexachloro-p-xylene—On the Catalytic Properties of Titanium–Magnesium Catalysts in the Homo- and Copolymerization of Ethylene with 1-Hexene
Authors: Salakhov Ildar I. , Mikenas Tatiana B. , Zakharov Vladimir A. , Kozlov Valeriy G. , Matsko Mikhail A. , Suslova Tatiana N.
Published: September 2022

Ethylene polymerization and ethylene/1-hexene copolymerization over the titanium–magnesium catalytic system in the presence of chlorocyclohexane (CHC) and hexachloro-p-xylene (HCPX) has been studied. Modification of TMC with chlorocyclohexane and hexachloro-p-xylene increased catalyst activity severalfold for both ethylene polymerization and ethylene/1-hexene copolymerization. The key kinetic regularities of ethylene homopolymerization and ethylene/1-hexene copolymerization in the presence of CHC and HCPX were determined, and the copolymerization constants were measured. Molecular characteristics and the copolymer composition were determined for the synthesized samples of ethylene homopolymers and ethylene/hexene copolymers. Modification of the titanium–magnesium catalyst with chlorinated organic compounds reduced 1-hexene content in the copolymer; polymerization was sensitive to 1-hexene as a regulator of polymer molecular weight. The potential mode of action of chlorinated organic modifiers on catalytic properties of titanium–magnesium catalyst is discussed.
Rosemary Extract-Induced Autophagy and Decrease in Accumulation of Collagen Type I in Osteogenesis Imperfecta Skin Fibroblasts
Authors: Sutkowska-Skolimowska Joanna , Brańska-Januszewska Justyna , Strawa Jakub W. , Ostrowska Halina , Botor Malwina , Gawron Katarzyna , Galicka Anna
Published: September 2022

Osteogenesis imperfecta (OI) is a heterogeneous connective tissue disease mainly caused by structural mutations in type I collagen. Mutant collagen accumulates intracellularly, causing cellular stress that has recently been shown to be phenotype-related. Therefore, the aim of the study was to search for potential drugs reducing collagen accumulation and improving OI fibroblast homeostasis. We found that rosemary extract (RE), which is of great interest to researchers due to its high therapeutic potential, at concentrations of 50 and 100 µg/mL significantly reduced the level of accumulated collagen in the fibroblasts of four patients with severe and lethal OI. The decrease in collagen accumulation was associated with RE-induced autophagy as was evidenced by an increase in the LC3-II/LC3-I ratio, a decrease in p62, and co-localization of type I collagen with LC3-II and LAMP2A by confocal microscopy. The unfolded protein response, activated in three of the four tested cells, and the level of pro-apoptotic markers (Bax, CHOP and cleaved caspase 3) were attenuated by RE. In addition, the role of RE-modulated proteasome in the degradation of unfolded procollagen chains was investigated. This study provides new insight into the beneficial effects of RE that may have some implications in OI therapy targeting cellular stress.
Effects of Global and Specific DNA-Binding Proteins on Transcriptional Regulation of the E. coli bgl Operon
Authors: Tran Dennis , Zhang Zhongge , Lam Katie Jing Kay , Saier Milton H.
Published: September 2022

Using reporter gene (lacZ) transcriptional fusions, we examined the transcriptional dependencies of the bgl promoter (Pbgl) and the entire operon regulatory region (Pbgl-bglG) on eight transcription factors as well as the inducer, salicin, and an IS5 insertion upstream of Pbgl. Crp-cAMP is the primary activator of both Pbgl and the bgl operon, while H-NS is a strong dominant operon repressor but only a weak repressor of Pbgl. H-NS may exert its repressive effect by looping the DNA at two binding sites. StpA is a relatively weak repressor in the absence of H-NS, while Fis also has a weak repressive effect. Salicin has no effect on Pbgl activity but causes a 30-fold induction of bgl operon expression. Induction depends on the activity of the BglF transporter/kinase. IS5 insertion has only a moderate effect on Pbgl but causes a much greater activation of the bgl operon expression by preventing the full repressive effects of H-NS and StpA. While several other transcription factors (BglJ, RcsB, and LeuO) have been reported to influence bgl operon transcription when overexpressed, they had little or no effect when present at wild type levels. These results indicate the important transcriptional regulatory mechanisms operative on the bgl operon in E. coli.
Effect of Different Molecular Weights of Polyacrylic Acid on Rat Lung Following Intratracheal Instillation
Authors: Nishida Chinatsu , Izumi Hiroto , Tomonaga Taisuke , Wang Ke-Yong , Higashi Hidenori , Takeshita Jun-Ichi , Ono Ryohei , Sumiya Kazuki , Fujii Shota , Hata Yuki , Sakurai Kazuo , Higashi Yasuyuki , Yamasaki Kei , Yatera Kazuhiro , Morimoto Yasuo
Published: September 2022

Background: We conducted intratracheal instillations of different molecular weights of polyacrylic acid (PAA) into rats in order to examine what kinds of physicochemical characteristics of acrylic acid-based polymer affect responses in the lung. Methods: F344 rats were intratracheally exposed to a high molecular weight (HMW) of 598 thousand g/mol or a low molecular weight (LMW) of 30.9 thousand g/mol PAA at low and high doses. Rats were sacrificed at 3 days, 1 week, 1 month, 3 months and 6 months post exposure. Results: HMW PAA caused persistent increases in neutrophil influx, cytokine-induced neutrophil chemoattractants (CINC) in the bronchoalveolar lavage fluid (BALF), and heme oxygenase-1 (HO-1) in the lung tissue from 3 days to 3 months and 6 months following instillation. On the other hand, LMW PAA caused only transient increases in neutrophil influx, CINC in BALF, and HO-1 in the lung tissue from 3 days to up to 1 week or 1 month following instillation. Histopathological findings of the lungs demonstrated that the extensive inflammation and fibrotic changes caused by the HMW PAA was greater than that in exposure to the LMW PAA during the observation period. Conclusion: HMW PAA induced persistence of lung disorder, suggesting that molecular weight is a physicochemical characteristic of PAA-induced lung disorder.