bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluation of Adverse Drug Reactions in Paediatric Patients: A Retrospective Study in Turkish Hospital
Authors: Khan Zakir , Karataş Yusuf , Kıroğlu Olcay
Published: November 2021

Drug safety in paediatric patients is a serious public health concern around the world. The paediatric patients are more prone to adverse drug reactions (ADRs) than adults. Moreover, there is a scarcity of information about ADRs in paediatric patients. This study was conducted to determine the frequency, causality, severity, preventability of paediatric patients’ ADRs reported in a tertiary care hospital in Adana, Turkey. A retrospective study was conducted on all spontaneously reported ADRs between January 01, 2020, to July 30, 2021, in paediatric patients. The ADRs reports were evaluated in terms of gender, age, ADR characteristics, suspected drugs and reporting source. All included ADRs reports were characterized according to the Naranjo Algorithm/World Health Organization (WHO) causality scales, Hartwig/Siegel and Common Terminology Criteria for Adverse Events (CTCAE) severity scales, the modified Schoumock and Thornton preventability scale and hospital pharmacovigilance center criteria for seriousness. Therapeutic groups were also coded using the WHO-Anatomical Therapeutic and Chemical (ATC) classification. During the study period, 8,912 paediatric patients who were admitted had 16 ADRs with 1.7 ADRs/1,000 admissions. The majority of ADRs were found in infants (31.2%) and children (56.2%) as compared to adolescents (12.5%). ADRs were observed more in females (81.2%) than males. Skin (62.5%) was the most affected organ due to the ADRs, and maculopapular rash and erythema multiforme were the most commonly reported symptoms. Most ADRs were probable/likely (93.7%), severe (50%), preventable or probably preventable (43.7%) and serious (37.5%). Antibiotics (93.7%) were found to be the most common cause of ADRs in paediatric patients. The majority of ADRs were associated with vancomycin (68.7%). Most of the ADRs were reported by a medical doctor in this study. This small sample size study highlights significant problems of ADRs in paediatric patients, mainly caused by antibiotics and with a majority of ADRs manifest as skin reactions. Furthermore, a high proportion of the identified ADRs were found to be preventable. More focused efforts are needed at the national level to avoid preventable ADRs in hospitals. Monitoring and management of ADRs and future studies would be beneficial for better patient care and safety.
Endurance Trained Athletes Do Not per se Have Higher Hoffmann Reflexes Than Recreationally Active Controls
Authors: Bertschinger Raphael , Giboin Louis-Solal , Gruber Markus
Published: November 2021

The impact of endurance training on spinal neural circuitries remains largely unknown. Some studies have reported higher H-reflexes in endurance trained athletes and therefore, adaptations within the Ia afferent pathways after long term endurance training have been suggested. In the present study we tested the hypothesis that cyclists (n = 12) demonstrate higher Hoffmann reflexes (H-reflexes) compared to recreationally active controls (n = 10). Notwithstanding, highly significant differences in endurance performance (VO2peak: 60.6 for cyclists vs. 46.3 ml/min/kg for controls (p < 0.001) there was no difference in the size of the SOL H-reflex between cyclists and controls (Hmax/Mmax ratio 61.3 vs. 60.0%, respectively (p = 0.840). Further analyses of the H and M recruitment curves for SOL revealed a significant steeper slope of the M recruitment curve in the group of cyclists (76.2 ± 3.8° vs. 72.0 ± 4.4°, p = 0.046) without a difference in the H-recruitment curve (84.6 ± 3.0° vs. 85.0 ± 2.8°, p = 0.784) compared to the control group. Cycling is classified as an endurance sport and thus the findings of the present study do not further support the assumption that long-term aerobic training leads to a general increase of the H-reflex. Amongst methodological differences in assessing the H-reflex, the training-specific sensorimotor control of the endurance sport itself might differently affect the responsiveness of spinal motoneurons on Ia-afferent inputs.
Exercise Heat Acclimation With Dehydration Does Not Affect Vascular and Cardiac Volumes or Systemic Hemodynamics During Endurance Exercise
Authors: Travers Gavin , González-Alonso José , Riding Nathan , Nichols David , Shaw Anthony , Périard Julien D.
Published: November 2021

Permissive dehydration during exercise heat acclimation (HA) may enhance hematological and cardiovascular adaptations and thus acute responses to prolonged exercise. However, the independent role of permissive dehydration on vascular and cardiac volumes, ventricular-arterial (VA) coupling and systemic hemodynamics has not been systematically investigated. Seven males completed two 10-day exercise HA interventions with controlled heart rate (HR) where euhydration was maintained or permissive dehydration (-2.9 ± 0.5% body mass) occurred. Two experimental trials were conducted before and after each HA intervention where euhydration was maintained (-0.5 ± 0.4%) or dehydration was induced (-3.6 ± 0.6%) via prescribed fluid intakes. Rectal (Tre) and skin temperatures, HR, blood (BV) and left ventricular (LV) volumes, and systemic hemodynamics were measured at rest and during bouts of semi-recumbent cycling (55% V̇O2peak) in 33°C at 20, 100, and 180 min. Throughout HA sweat rate (12 ± 9%) and power output (18 ± 7 W) increased (P < 0.05), whereas Tre was 38.4 ± 0.2°C during the 75 min of HR controlled exercise (P = 1.00). Neither HA intervention altered resting and euhydrated exercising Tre, BV, LV diastolic and systolic volumes, systemic hemodynamics, and VA coupling (P > 0.05). Furthermore, the thermal and cardiovascular strain during exercise with acute dehydration post-HA was not influenced by HA hydration strategy. Instead, elevations in Tre and HR and reductions in BV and cardiac output matched pre-HA levels (P > 0.05). These findings indicate that permissive dehydration during exercise HA with controlled HR and maintained thermal stimulus does not affect hematological or cardiovascular responses during acute endurance exercise under moderate heat stress with maintained euhydration or moderate dehydration.
Transcriptomics and Metabolomics Changes Triggered by Inflorescence Removal in Panax notoginseng (Burk.)
Authors: Bai Yu , Liu Haijiao , Pan Jianying , Zhang Shiyan , Guo Yixin , Xian Yisha , Sun Zhirong , Zhang Zilong
Published: November 2021

The root of Panax notoginseng (Burk.), in which saponins are the major active components, is a famous traditional Chinese medicine used to stop bleeding and to decrease inflammation and heart disease. Inflorescence removal increases the yield and quality of P. notoginseng, but the underlying molecular mechanisms are unknown. Here, the differences between inflorescence-removal treatment and control groups of P. notoginseng were compared using transcriptomics and metabolomics analyses. Illumina sequencing of cDNA libraries prepared from the rhizomes, leaves and roots of the two groups independently identified 6,464, 4,584, and 7,220 differentially expressed genes (DEG), respectively. In total, 345 differentially expressed transcription factors (TFs), including MYB and WRKY family members, were induced by the inflorescence-removal treatment. Additionally, 215 DEGs involved in saponin terpenoid backbone biosynthetic pathways were identified. Most genes involved in the mevalonic acid (MVA) and methylerythritol phosphate (MEP) pathways were activated by inflorescence removal. The co-expression analysis showed that the low expression levels of flavonoid biosynthesis-related genes (e.g., C4H and F3H) decreased the biosynthesis and accumulation of some flavonoids after inflorescence removal. The results not only provide new insights into the fundamental mechanisms underlying the poorly studied inflorescence-removal process in P. notoginseng and other rhizome crops, but they also represent an important resource for future research on gene functions during inflorescence-removal treatments and the reproductive stage.
On the Effect of Practice on Exploration and Exploitation of Options and Strategies
Authors: Cohen Doron , Teodorescu Kinneret
Published: November 2021

Insufficient exploration of one’s surroundings is at the root of many real-life problems, as demonstrated by many famous biases (e.g., the status quo bias, learned helplessness). The current work focuses on the emergence of this phenomenon at the strategy level: the tendency to under-explore the set of available choice strategies. We demonstrate that insufficient exploration of strategies can also manifest as excessive exploration between options. In such cases, interventions aimed at improving choices by reducing the costs of exploration of options are likely to fail. In Study 1, participants faced an exploration task that implies an infinite number of choice strategies and a small sub-set of (near) optimal solutions. We manipulated the amount of practice participants underwent during the first, shorter game and compared their performance in a second, longer game with an identical payoff structure. Our results show that regardless of the amount of practice, participants in all experimental groups tended to under-explore the strategy space and relied on a specific strategy that implied over-exploration of the option space. That is, under-exploration of strategies was manifested as over-exploration of options. In Study 2, we added a constraint that, on a subset of practice trials, forced participants to exploit familiar options. This manipulation almost doubled the per-trial average outcome on the comparable longer second game. This suggests that forcing participants to experience the effects of different (underexplored) strategy components during practice can greatly increase the chance they make better choices later on.
Factor Structure and Psychometric Properties of the Family Communication Scale in the Chinese Population
Authors: Guo Ningyuan , Ho Henry C. Y. , Wang Man Ping , Lai Agnes Y. , Luk Tzu Tsun , Viswanath Kasisomayajula , Chan Sophia S. , Lam Tai Hing
Published: November 2021

Purpose: To evaluate the factor structure and psychometric properties of the 10-item Family Communication Scale (FCS) in the Chinese population.

Methods: Study 1 was a population-based survey [N = 687, 61.1% female; mean age (SD) 56.6 (19.1)]. Study 2 was a community-based intervention (N = 1983, 76.7% female; 57.8% aged 20–59 years). We conducted exploratory factor analysis (EFA) in Study 1 and replicated the model by confirmatory factor analysis (CFA) in Study 2. Psychometric properties were evaluated, including internal consistency, test–retest reliability, convergent and discriminant validity, and known-group validity. We identified how the FCS scores differed by sociodemographic characteristics and communication methods including face to face and Information and Communication Technologies (ICTs) in Study 1.

Results: The EFA and CFA supported a one-factor structure. The Chinese FCS showed a good internal consistency (Cronbach’s alpha = 0.91; McDonald’s Omega = 0.91) and was stable over 1-month (intraclass correlation coefficient = 0.69, P < 0.001). Convergent validity was supported by positive correlations of FCS with the Subjective Happiness Scale, Family Adaption, Partnership, Growth, Affection, Resolve (APGAR) Scale, family health, harmony, and happiness, and perceived family communication sufficiency and quality (All P < 0.001). Discriminant validity was supported by the stronger correlation of FCS with Short Form-12 Health Survey Version 2 Mental Component than that with Physical Component (P < 0.001). Higher household income, frequent face-to-face communication, and frequent use of phone calls, instant messaging, and social networking sites were associated with higher FCS scores.

Conclusion: The one-factor structure of the Chinese FCS can be a reliable and valid measurement of positive family communication, in the context of ICT integration into family communication.

Clinical Trial Registration: [www.ClinicalTrials.gov], identifier [NCT02563613].
Decision-Making Support for People With Alzheimer’s Disease: A Narrative Review
Authors: Sun Weiyi , Matsuoka Teruyuki , Narumoto Jin
Published: November 2021

The proportion of people with dementia has been increasing yearly, and the decision-making capacity of these people has become a major concern in fields such as the financial industry and in medical settings. In this narrative review, we discuss decision-making in people with Alzheimer’s disease (AD), and we propose the support for decision-making in people with AD, especially financial and medical decision-making. We summarize several hypotheses and theories on the decision-making capacity of people with AD. These include the frontal lobe hypothesis, physiological theory, dysfunction of the hypothalamic-pituitary-adrenal (HPA) axis, and the Person-Task-Fit (PTF) framework. Both internal and external factors can affect decision-making by people with AD. Internal factors are affected by changes in the brain and neurotransmitters, as well as alterations in cognitive ability and emotion. External factors include task characters, task contents, and situation influence. Since feedback has a significant effect on decision-making capacity, a series of suggestions may be helpful to improve this capacity, such as explicit advice, simple options, pleasant rewards, the Talking Mats approach, memory and organizational aid, support by caregivers, cognitive training and feedback. Thus, in providing decision-making support for people with AD, it is important to identify the internal and external factors that impair this process and to deal with these factors.
Self-Medication for Chronic Pain Using Classic Psychedelics: A Qualitative Investigation to Inform Future Research
Authors: Bornemann Julia , Close James B. , Spriggs Meg J. , Carhart-Harris Robin , Roseman Leor
Published: November 2021

Background:Chronic Pain is among the leading causes of disability worldwide with up to 60% of patients suffering from comorbid depression. Psychedelic-assisted therapy has recently been found effective in treating a host of mental health issues including depression and has historically been found to be useful in treating pain. Reports of self-medication for chronic pain using psychedelic drugs have been widely documented, with anecdotal evidence indicating widespread success in a range of pathologies.

Aims:In preparation for an upcoming trial, to better understand how those with lived experience of chronic pain self-medicate with psychedelic drugs, and to establish, in detail, their therapeutic protocols and practices for success.

Methods:As part of patient-involvement (PI) for an upcoming trial in this population, 11 individuals who reported self-medicating with psychedelic drugs took part in a 1-h semi-structured discussion, which was then transcribed and thematically analyzed.

Results:Across a range of psychedelic substances and doses, reported pain scores improved substantially during and after psychedelic experiences. Two processes, Positive Reframing and Somatic Presence, were reliably identified as playing a role in improvements in mental wellbeing, relationship with pain, and physical (dis)comfort. Inclusion of other strategies such as mindfulness, breathwork, and movement were also widely reported. Due to the data's subjective nature, this paper is vulnerable to bias and makes no claims on causality or generalisability. Together, these results have been used to inform study design for a forthcoming trial.

Conclusion:This pre-trial PI work gives us confidence to test psychedelic therapy for chronic pain in a forthcoming controlled trial. The results presented here will be instrumental in improving our ability to meet the needs of future study participants.
Preliminary Study on Changes of Sleep EEG Power and Plasma Melatonin in Male Patients With Major Depressive Disorder After 8 Weeks Treatment
Authors: Wang Xue-Qin , Wang De-Quan , Bao Yan-Ping , Liu Jia-Jia , Chen Jie , Wu Shao-Wei , Luk Hsuan-Nu , Yu Ling , Sun Wei , Yang Yong , Wang Xue-Hua , Lu Lin , Deng Jia-Hui , Li Su-Xia
Published: November 2021

Objective: To clarify the effects of escitalopram on sleep EEG power in patients with Major depressive disorder (MDD).

Method: Polysomnography (PSG) was detected overnight, and blood samples were collected at 4 h intervals over 24 h from 13 male healthy controls and 13 male MDD patients before and after treatment with escitalopram for 8 weeks. The outcome measures included plasma melatonin levels, sleep architecture, and the sleep EEG power ratio.

Results: Compared with healthy controls, MDD patients presented abnormalities in the diurnal rhythm of melatonin secretion, including peak phase delayed 3 h and a decrease in plasma melatonin levels at night and an increase at daytime, accompanied by sleep disturbances, a decrease in low-frequency bands and an increase in high-frequency bands, and the dominant right-side brain activity. Several of these abnormalities (abnormalities in the diurnal rhythm of melatonin secretion, partial sleep architecture parameters) persisted for at least the 8-week testing period.

Conclusions: Eight weeks of treatment with escitalopram significantly improved subjective sleep perception and depressive symptoms of patients with MDD, and partially improved objective sleep parameters, while the improvement of circadian rhythm of melatonin was limited.
Brain Entropy Study on Obsessive-Compulsive Disorder Using Resting-State fMRI
Authors: Jiang Xi , Li Xue , Xing Haoyang , Huang Xiaoqi , Xu Xin , Li Jing
Published: November 2021

Object: Brain entropy is a potential index in the diagnosis of mental diseases, but there are some differences in different brain entropy calculation, which may bring confusion and difficulties to the application of brain entropy. Based on the resting-state function magnetic resonance imaging (fMRI) we analyzed the differences of the three main brain entropy in the statistical significance, including approximate entropy (ApEn), sample entropy (SampEn) and fuzzy entropy (FuzzyEn), and studied the physiological reasons behind the difference through comparing their performance on obsessive-compulsive disorder (OCD) and the healthy control (HC).

Method: We set patients with OCD as the experimental group and healthy subjects as the control group. The brain entropy of the OCD group and the HC are calculated, respectively, by voxel and AAL region. And then we analyzed the statistical differences of the three brain entropies between the patients and the control group. To compare the sensitivity and robustness of these three kinds of entropy, we also studied their performance by using certain signal mixed with noise.

Result: Compare with the control group, almost the whole brain's ApEn and FuzzyEn of OCD are larger significantly. Besides, there are more brain regions with obvious differences when using ApEn comparing to using FuzzyEn. There was no statistical difference between the SampEn of OCD and HC.

Conclusion: Brain entropy is a numerical index related to brain function and can be used as a supplementary biological index to evaluate brain state, which may be used as a reference for the diagnosis of mental illness. According to an analysis of certain signal mixed with noise, we conclude that FuzzyEn is more accurate considering sensitivity, stability and robustness of entropy.
Parent-Mediated Interventions for Children and Adolescents With Autism Spectrum Disorders: A Systematic Review and Meta-Analysis
Authors: Conrad Charlotte Engberg , Rimestad Marie Louise , Rohde Jeanett Friis , Petersen Birgitte Holm , Korfitsen Christoffer Bruun , Tarp Simon , Cantio Cathriona , Lauritsen Marlene Briciet , Händel Mina Nicole
Published: November 2021

There has been increasing interest in parent-mediated interventions (PMIs) for children with autism spectrum disorders (ASDs). The objective of this systematic review and meta-analysis was to examine the effect of PMIs compared to no PMI for children with ASD aged 2–17 years. The primary outcome was adaptive functioning rated by a parent or clinician. The secondary outcomes were long-term adaptive functioning rated by the parents, adverse events, core symptoms of ASD, disruptive behavior, parental well-being, quality of life of the child rated by the parents and anxiety. The MEDLINE, PsycInfo, Embase, and CINAHL databases were searched in March 2020. The Cochrane Risk of Bias Tool was used to rate the individual studies, and the certainty in the evidence was evaluated using GRADE. We identified 30 relevant randomized controlled trials (RCTs), including 1,934 participants. A clinically relevant effect of PMIs on parent-rated adaptive functioning was found with a low certainty of evidence [Standard mean difference (SMD): 0.28 (95% CI: −0.01, 0.57)] on Vineland Adaptive Behavior Scales (VABS), whereas no clinically relevant effect was seen for clinician-rated functional level, with a very low certainty of evidence [SMD on Clinical Global Impressions (CGI)-severity scale: SMD −0.45 [95% CI: −0.87, −0.03)]. PMIs may slightly improve clinician-rated autism core symptoms [SMD: −0.35 (95% CI: −0.71, 0.02)]. Additionally, no effect of PMIs on parent-rated core symptoms of ASD, parental well-being or adverse effects was identified, all with a low certainty of evidence. There was a moderate certainty of evidence for a clinically relevant effect on disruptive behavior [SMD: 0.55 (95% Cl: 0.36, 0.74)]. The certainty in the evidence was downgraded due to serious risk of bias, lack of blinding, and serious risk of imprecision due to few participants included in meta-analyses. The present findings suggest that clinicians may consider introducing PMIs to children with ASD, but more high-quality RCTs are needed because the effects are not well-established, and the results are likely to change with future studies. The protocol for the systematic review is registered at the Danish Health Authority website (www.sst.dk).
Role of the Media in Health-Related Awareness Campaigns on Perception of COVID-19: A Pre-post Study in the General Population of Pakistan
Authors: Rehman Atta Ur , Zakar Rubeena , Zakar Muhammad Zakria , Hani Ume , Wrona Kamil J. , Fischer Florian
Published: November 2021

Medical preparedness and community education are the most valuable preventive tools for combatting the COVID-19 pandemic. This study aims to assess the role of media public health awareness campaigns on the knowledge of the general population about COVID-19 in Rawalpindi, Pakistan. A quantitative study using a pre-post design among 384 respondents was conducted. A structured questionnaire was administered to the participants twice: The first response (t1) from participants was filled in during the 1st week in February 2020 before any confirmed cases were reported in the country, and the second response (t2) was completed 1 month after the first case detection in Pakistan (March 2020). Media health awareness campaigns were launched just after the detection of the first case in Pakistan. Exposure to the media and knowledge relating to COVID-19 increased over time. Whereas, only a quarter of respondents judged the isolation of suspected cases in quarantine to be important to prevent the spread of infection in society at t1, more than half did so at t2. Socio-demographic characteristics were not significantly associated with knowledge (gains). However, more frequent use of electronic media is associated with greater knowledge gains from t1 to t2. The findings of this study provide evidence that awareness and knowledge related to COVID-19 symptoms and preventive measures increased significantly over time. The increased frequency of following the media indicates that health awareness campaigns are important for enhancing the knowledge of the general public regarding COVID-19.
Environing as Embodied Experience—A Study of Outdoor Education as Part of Physical Education
Authors: Lundvall Suzanne , Maivorsdotter Ninitha
Published: November 2021

The development of a re-understanding or re-investigation of body pedagogy is currently prominent in the field of physical education (PE) and sport pedagogy. This goes for the learning of movement capability and health but also in relation to outdoor education (OE). The latter a criticized area for having a one-size-fits-all approach to curriculum, with less attention to what to learn in OE, including aspects of everyday practices of being outdoors. The aim of this study was to explore students aged 15 years, and their meaning making of being outdoors expressed in written stories about a favorite place. Two school year eight classes in a Swedish compulsory school situated in an area with high diversity participated. Through this theory-generated empirical study, written stories were explored as one way of evaluating students' meaning making of outdoor places. By using practical epistemology analysis (PEA) to examine experience operationalized through aesthetic judgements attention is paid to the relation between the student and the situation (their favorite place). The analysis make it possible to discern a sense and meaning making of “being” outdoors as an embodied experience, as a relational whole of the self, others and the environment. Descriptions of aesthetic experiences were analyzed leading to dimensions of environing described as “calm and privacy,” “community and togetherness” and “feelings and senses.” A favorite place was by all students described as a very local and nearby place accessible in everyday life. The analysis generated understandings of feelings of “fulfillment” and different embodied experiences of what an encounter with an outdoor place or being outdoors could mean. Furthermore, how personal and diverse the meaning making place tends to be and how experience and habits contribute to the students' creation of microenvironments. Dimensions of environing become part of an embodied process. The analysis of the written stories calls for an alternative understanding of what OE can or should consist of. The findings encourage teachers and researchers to consider alternative understandings and practices of OE that highlight and educate students' overall embodied (individual) experiences and learning in OE and PE.
Disease Risk and Conservation Implications of Orangutan Translocations
Authors: Sherman Julie , Unwin Steve , Travis Dominic A. , Oram Felicity , Wich Serge A. , Jaya Ricko L. , Voigt Maria , Santika Truly , Massingham Emily , Seaman Dave J. I. , Meijaard Erik , Ancrenaz Marc
Published: November 2021

Critically Endangered orangutans are translocated in several situations: reintroduced into historic range where no wild populations exist, released to reinforce existing wild populations, and wild-to-wild translocated to remove individuals from potentially risky situations. Translocated orangutans exposed to human diseases, including Coronavirus Disease 2019 (COVID-19), pose risks to wild and previously released conspecifics. Wildlife disease risk experts recommended halting great ape translocations during the COVID-19 pandemic to minimize risk of disease transmission to wild populations. We collected data on orangutan releases and associated disease risk management in Indonesia during the COVID-19 pandemic, and developed a problem description for orangutan disease and conservation risks. We identified that at least 15 rehabilitated ex-captive and 27 wild captured orangutans were released during the study period. Identified disease risks included several wild-to-wild translocated orangutans in direct contact or proximity to humans without protective equipment, and formerly captive rehabilitated orangutans that have had long periods of contact and potential exposure to human diseases. While translocation practitioners typically employ mitigation measures to decrease disease transmission likelihood, these measures cannot eliminate all risk, and are not consistently applied. COVID-19 and other diseases of human origin can be transmitted to orangutans, which could have catastrophic impacts on wild orangutans, other susceptible fauna, and humans should disease transmission occur. We recommend stakeholders conduct a Disease Risk Analysis for orangutan translocation, and improve pathogen surveillance and mitigation measures to decrease the likelihood of potential outbreaks. We also suggest refocusing conservation efforts on alternatives to wild-to-wild translocation including mitigating human-orangutan interactions, enforcing laws and protecting orangutan habitats to conserve orangutans in situ.
The Influence of an Enriched Environment in Enhancing Recognition Memory in Zebrafish (Danio rerio)
Authors: DePasquale Cairsty , Kemerer Nicole , White Nathan , Yost Monica , Wolfkill Jordan , Sturgill Jennifer , Li X.
Published: November 2021

Environmental enrichment is used to increase social and physical stimulation for animals in captivity which can lead to enhanced cognition. Fundamental to the positive effect enrichment has on the brain is that it provides opportunities for captive animals to recognize and discriminate between different stimuli in the environment. In the wild, being able to discriminate between novel or familiar stimuli has implications for survival, for example finding food, hiding from predators, or even choosing a mate. The novel object recognition (NOR) test is a cognitive task that is used extensively in the rodent literature to assess object recognition and memory, where the amount of time an animal spends exploring a novel vs. familiar object is quantified. Enrichment has been shown to enhance object recognition in rodents. More recently, the use of the NOR test has been applied to another animal model, zebrafish (Danio rerio), however, the effects of enrichment have not yet been explored. In the current study we looked at the effects of enrichment on object recognition in zebrafish using the NOR test. Adult zebrafish were housed in either enriched conditions (gravel substrate, plastic plants, shelter, heater and a filter) or plain conditions (heater and filter only) for 6 months before behavioral NOR tests were conducted. Enriched fish showed a preference for a novel object over a familiar one at a distance but did not show a preference during close inspection. Control fish did not show a preference at either distance. Our results suggest that enrichment can enhance zebrafish ability to discriminate between novel and familiar objects, but distance from the object may be an important factor. Future research is needed to determine whether any enhancements in object recognition are a result of an increase in sensory stimulation from being reared with enrichment, or whether it is due to a reduction in stress reactivity.
Development of a New LAMP Assay for the Detection of Ancylostoma caninum DNA (Copro-LAMPAc) in Dog Fecal Samples
Authors: Avila Héctor Gabriel , Risso Marikena Guadalupe , Cabrera Marta , Ruybal Paula , Repetto Silvia Analía , Butti Marcos Javier , Trangoni Marcos David , Santillán Graciela , Pérez Verónica Mirtha , Periago María Victoria
Published: November 2021

Ancylostoma caninum is a zoonotic nematode which is able to affect animals and humans. Diagnosis in the definitive host and environmental detection are key to prevent its dissemination and achieve control. Herein, a new coprological LAMP method for the detection of A. caninum (Copro-LAMPAc) DNA was developed. DNA extraction was performed using a low-cost method and a fragment of the cox-1 gene was used for primer design. The analytical sensitivity, evaluated with serial dilutions of genomic DNA from A. caninum adult worms, was 100 fg. A specificity of 100% was obtained using genomic DNA from the host and other pathogens. The Copro-LAMPAc was evaluated using environmental canine fecal samples. When compared with gold standard optical microscopy in epidemiological studies, it proved to be more sensitive. This new LAMP assay can provide an alternative protocol for screening and identification of A. caninum for epidemiological studies in endemic areas.
Vitrification Effects on the Transcriptome of in vivo-Derived Porcine Morulae
Authors: Cuello Cristina , Martinez Cristina A. , Cambra Josep M. , González-Plaza Alejandro , Parrilla Inmaculada , Rodriguez-Martinez Heriberto , Gil Maria A. , Martinez Emilio A.
Published: November 2021

Despite the reported promising farrowing rates after non-surgical and surgical transfers of vitrified porcine morulae and blastocysts produced in vivo (range: 70–75%), the pregnancy loss is 5–15 fold higher with vitrified than with fresh embryos. The present study aimed to investigate whether vitrification affects the transcriptome of porcine morulae, using microarrays and RT-qPCR validation. Morulae were obtained surgically from weaned sows (n = 13) on day 6 (day 0 = estrus onset). A total of 60 morulae were vitrified (treatment group). After 1 week of storage, the vitrified morulae were warmed. Vitrified-warmed and non-vitrified fresh morulae (control; n = 40) were cultured for 24 h to assess embryo survival by stereomicroscopy after. A total of 30 vitrified/warmed embryos that were deemed viable and 30 fresh control embryos (three pools of 10 for each experimental group) were selected for microarray analysis. Gene expression was assessed with a GeneChip® Porcine Genome Array (Affymetrix). An ANOVA analysis p-unadjusted <0.05 and a fold change cut-off of ±1.5 were set to identify differentially expressed genes (DEGs). Data analysis and biological interpretation were performed using the Partek Genomic Suite 7.0 software. The survival rate of morulae after vitrification and warming (92.0 ± 8.3%) was similar to that of the control (100%). A total of 233 DEGs were identified in vitrified morulae (38 upregulated and 195 downregulated), compared to the control group. Nine pathways were significantly modified. Go-enrichment analysis revealed that DEGs were mainly related to the Biological Process functional group. Up-regulated DEGs were involved in glycosaminoglycan degradation, metabolic pathways and tryptophan metabolism KEGG pathways. The pathways related to the down-regulated DEGs were glycolysis/gluconeogenesis, protein export and fatty acid elongation. The disruption of metabolic pathways in morulae could be related to impaired embryo quality and developmental potential, despite the relatively high survival rates after warming observed in vitro. In conclusion, vitrification altered the gene expression pattern of porcine morulae produced in vivo, generating alterations in the transcriptome that may interfere with subsequent embryo development and pregnancy after embryo transfer.
Simulation of a GOx-GCH4 Rocket Combustor and the Effect of the GEKO Turbulence Model Coefficients
Authors: Strokach Evgeny , Zhukov Victor , Borovik Igor , Sternin Andrej , Haidn Oscar J.
Published: November 2021

In this study, a single injector methane-oxygen rocket combustor is numerically studied. The simulations included in this study are based on the hardware and experimental data from the Technical University of Munich. The focus is on the recently developed generalized k–ω turbulence model (GEKO) and the effect of its adjustable coefficients on the pressure and on wall heat flux profiles, which are compared with the experimental data. It was found that the coefficients of ‘jet’, ‘near-wall’, and ‘mixing’ have a major impact, whereas the opposite can be deduced about the ‘separation’ parameter Csep, which highly influences the pressure and wall heat flux distributions due to the changes in the eddy-viscosity field. The simulation results are compared with the standard k–ε model, displaying a qualitatively and quantitatively similar behavior to the GEKO model at a Csep equal to unity. The default GEKO model shows a stable performance for three oxidizer-to-fuel ratios, enhancing the reliability of its use. The simulations are conducted using two chemical kinetic mechanisms: Zhukov and Kong and the more detailed RAMEC. The influence of the combustion model is of the same order as the influence of the turbulence model. In general, the numerical results present a good or satisfactory agreement with the experiment, and both GEKO at Csep = 1 or the standard k–ε model can be recommended for usage in the CFD simulations of rocket combustion chambers, as well as the Zhukov–Kong mechanism in conjunction with the flamelet approach.
Floristic Composition: Dynamic Biodiversity Indicator of Tree Canopy Effect on Dryland and Improved Mediterranean Pastures
Authors: Serrano João , Shahidian Shakib , Machado Eliana , Paniagua Luís L. , Carreira Emanuel , Moral Francisco , Pereira Alfredo , de Carvalho Mário
Published: November 2021

Montado is a characteristic ecosystem of the Mediterranean region. The adequate management of this silvo-pastoral ecosystem requires good understanding of the effect of factors such as tree canopy, fertilization and soil amendment on pasture growth. The main objectives of this work were: (1) to evaluate the effect of tree canopy on soil characteristics and pasture productivity and quality; and (2) to test floristic composition assessment as a bio-indicator of soil improvements (amendment and fertilization) in each study area (under and outside tree canopy). Topsoil was characterized at the beginning of the project (October 2015) and at the end of the experiments (spring 2020). Soil parameters obtained by electronic sensors (soil moisture content, soil cone index and surface temperature) were monitored monthly during the 2017/2018 pasture vegetative cycle. Pasture productivity, quality and floristic composition were evaluated every two years (2016, 2018 and 2020) in the spring flowering period. The results of the floristic inventory were submitted to a multilevel pattern analysis (Indicator Species Analysis, ISA). Pasture biodiversity was evaluated based on the calculation of richness indices. This study showed a positive effect of tree canopy on soil fertility and pasture quality (e.g., CP). Pasture productivity, on the other hand, was higher in areas outside tree canopy. The great potential of ISA as a tool for identification of bio-indicator species was also demonstrated. Pasture species were identified as ecological and dynamic attributes characteristic of each study area, before and after soil amendment and fertilization.
Assessment of Composted Pelletized Poultry Litter as an Alternative to Chemical Fertilizers Based on the Environmental Impact of Their Production
Authors: Kiss Nikolett Éva , Tamás János , Szőllősi Nikolett , Gorliczay Edit , Nagy Attila
Published: November 2021

Reducing the use of chemical fertilizers in agriculture is one of the EU Green Deal’s priorities. Since poultry production is increasing worldwide, stabilized poultry litter such as composted pelletized poultry litter (CPPL) is an alternative fertilizer option. On the contrary, compared to chemical fertilizers, the environmental impacts of composted products have not been adequately studied, and no data are currently available for CPPL produced by a closed composting system, such as the Hosoya system. The aim of this research was to assess the role of CPPL as a potential alternative for chemical fertilizer by evaluating the environmental impact of CPPL production via the Hosoya system using common chemical fertilizers. Based on life cycle assessment (LCA), the environmental impact (11 impact categories) was determined for the production of 1 kg of fertilizer, as well as for the production of 1 kg of active substances (nitrogen (N), phosphorus pentoxide (P2O5), and potassium chloride (K2O)) and the theoretical nutrient (NPK) supply of a 100 ha field with CPPL and several chemical fertilizer options. The production of CPPL per kilogram was smaller than that of the chemical fertilizers; however, the environmental impact of chemical fertilizer production per kilogram of active substance (N, P2O5, or K2O) was lower for most impact categories, because the active substance was available at higher concentrations in said chemical fertilizers. In contrast, the NPK supply of a 100 ha field by CPPL was found to possess a smaller environmental impact compared to several combinations of chemical fertilizers. In conclusion, CPPL demonstrated its suitability as an alternative to chemical fertilizers.
Soil Disturbance Impact on Crop Ergothioneine Content Connects Soil and Human Health
Authors: Beelman Robert B. , Richie John P. , Phillips Allen T. , Kalaras Michael D. , Sun Dongxiao , Duiker Sjoerd W.
Published: November 2021

Ergothioneine (ERGO) is a potent antioxidant and anti-inflammatory amino acid that is produced in nature mainly by non-yeast fungi, cyanobacteria, and mycobacteria. Mounting evidence suggests that ERGO can be considered a longevity vitamin that can mitigate chronic diseases of aging and thereby increase life expectancy. Humans must obtain ERGO from their diet, and it is therefore important to know which foods contain it. Although ERGO is not produced by plants it is found in plant products such as grain, apparently because detrital or symbiotic soil fungi pass on ERGO to plants through their roots. Besides differences between plant species in their ability to accumulate ERGO, how they are managed might also affect its concentration. Soil tillage has been shown to reduce soil fungal biomass, and therefore ERGO contents in maize, soybeans, and oats grown in soil managed with annual moldboard plowing (most intensive), chisel/disking (less intensive), or no-tillage (least intensive) in crop rotation were compared. ERGO concentrations declined in all three crops as tillage intensity increased, with reductions from no till to moldboard plow of approximately 30% in all three crops. Because crop yield was also negatively impacted by intensive tillage, ERGO yield per hectare was reduced even more due to increasing tillage intensity. This study is one of the first to show that soil health improving practices that minimize soil disturbance can directly enhance a key dietary factor associated with long-term human health.
Crop Wild Relatives Crosses: Multi-Location Assessment in Durum Wheat, Barley, and Lentil
Authors: El Haddad Noureddine , Sanchez-Garcia Miguel , Visioni Andrea , Jilal Abderrazek , El Amil Rola , Sall Amadou Tidiane , Lagesse Wasihun , Kumar Shiv , Bassi Filippo M.
Published: November 2021

Crop wild relatives (CWR) are a good source of useful alleles for climate change adaptation. Here, 19 durum wheat, 24 barley, and 24 lentil elites incorporating CWR in their pedigrees were yield tested against commercial checks across 19 environments located in Morocco, Ethiopia, Lebanon, and Senegal. For each crop, the combined analysis of variance showed that genotype (G), environment (E), and genotype x environment (G×E) effects were significant for most of the traits. A selection index combining yield potential (G) and yield stability (G×E) was used to identify six CWR-derived elites for each crop matching or superior to the best check. A regression analysis using a climate matrix revealed that grain yield was mostly influenced by the maximum daily temperature and soil moisture level during the growing stages. These climatic factors were used to define five clusters (i.e., E1 to E5) of mega-environments. The CWR-derived elites significantly outperformed the checks in E1, E2, and E4 for durum wheat, and in E2 for both barley and lentil. The germplasm was also assessed for several food transformation characteristics. For durum wheat, one accession (Zeina) originating from T. araraticum was significantly superior in mixograph score to the best check, and three accessions originating from T. araraticum and T. urartu were superior for Zn concentration. For barley, 21 accessions originating from H. spontaneum were superior to the checks for protein content, six for Zn content, and eight for β-glucan. For lentil, ten accessions originating from Lens orientalis were superior to the check for protein content, five for Zn, and ten for Fe concentration. Hence, the results presented here strongly support the use of CWR in breeding programs of these three dryland crops, both for adaptation to climatic stresses and for value addition for food transformation.
Coffee Rust Forecast Systems: Development of a Warning Platform in a Minas Gerais State, Brazil
Authors: Pozza Edson Ampélio , Santos Éder Ribeiro dos , Gaspar Nilva Alice , Vilela Ximena Maira de Souza , Alves Marcelo de Carvalho , Colares Mário Roberto Nogueira
Published: November 2021

This study aimed to develop a warning system platform for coffee rust incidence fifteen days in advance, as well as validating and regionalizing multiple linear regression models based on meteorological variables. The models developed by Pinto were validated in five counties. Experiments were set up in a randomized block design with five treatments and five replications. The experimental plot had six lines with 20 central plants of useful area. Assessments of coffee rust incidence were carried out fortnightly. The data collected from automatic stations were adjusted in new multiple linear regression models (MLRM) for five counties. Meteorological variables were lagged concerning disease assessment dates. After the adjustments, two models were selected and calculated for five counties, later there was an expansion to include ten more counties and 35 properties to validate these models. The result showed that the adjusted models of 15–30 days before rust incidence for Carmo do Rio Claro and Nova Resende counties were promising. These models were the best at forecasting disease 15 days in advance. With these models and the geoinformation systems, the warning platform and interface will be improved in the coffee grower region of the south and savannas of the Minas Gerais State, Brazil.
Metabolomics Analysis Reveals Potential Mechanisms in Bupleurum L. (Apiaceae) Induced by Three Levels of Nitrogen Fertilization
Authors: Sun Jialin , Li Weinan , Zhang Ye , Guo Yun , Duan Zejia , Tang Zhonghua , Abozeid Ann
Published: November 2021

Bupleurum (Apiaceae) is widely used in traditional Chinese medicine to treat inflammatory and infectious diseases. Although roots are the only used parts in China, other countries use the whole plant. The yield and quality of Bupleurum depend mainly on fertilizers, especially nitrogen. The current study aimed to assess the relationship between the nitrogen fertilization level and the quality and metabolomic response of different parts (flowers, main shoots, lateral shoots and roots) of Bupleurum to three nitrogen fertilization levels (control group: 0 kg·ha−1; low-nitrogen group: 55 kg·ha−1; high-nitrogen group: 110 kg·ha−1). The results showed that a high nitrogen level increases Bupleurum yield and quality parameters only in aerial parts, especially flowers, but has no significant effect on roots. The HPLC method was exploited for simultaneous quantification of three saikosaponins (A, C and D), which are the main bioactive components in the plant. It was found that the total content of saikosaponins decreased with high nitrogen fertilization in roots but significantly increased in flowers. Moreover, nitrogen fertilizer promoted the content of saikosaponin A but inhibited saikosaponins C and saikosaponins D in most parts of the plant. To study the response of primary metabolites, we adopted gas chromatography–mass spectrometry (GC−MS) analysis; 84 metabolites were identified that were mostly up-regulated with a high nitrogen level in flowers but down-regulated in roots. Four differential metabolites—D-fructose, lactose, ether and glycerol—were recognized as key metabolites in Bupleurum under nitrogen fertilization. Meanwhile, The Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment results explained that the impact of nitrogen fertilization on Bupleurum was attributed to the C-metabolism, N-metabolism, and lipids metabolism. This research put forward new insights into potential mechanisms and the relationship between the quality and yield of Bupleurum and nitrogen fertilization.