EXPERIMENTAL JUSTIFICATION OF THE EFFICIENCY OF USING THE IMMUNOMODULATOR «BLASTOMUNIL» IN THE TREATMENT OF I NFECTED WOUNDS
Authors: Khimich O. S. , Rautskis V. P. , Khimich S. D. , Pivtorak V. I. , Kryvonos M. I. 
Published: June 2024

Abstract. Introduction. Infected and purulent wounds are quite common in medical practice. And despite the fact that many successes have been achieved in the treatment of patients with such wounds, their treatment is still far from ideal due to many factors. One of the factors that worsens wound healing is the poor state of immunity of the macroorganism.
Goal. To study the effectiveness of using the immunomodulator "Blastomunil" in the treatment of infected and purulent wounds.
Research materials and methods. For the study, 65 white rats with a body weight of 180-200 g were selected. To carry out the work, 6 research groups were formed, each with 10 animals. All animals of groups I-VI were initially subjected to "ketamine anesthesia" by intramuscular injection of ketamine at the rate of 15 mg/kg of rat body weight. Under anesthesia, the animals were fixed by their limbs on the work table for preparation of the operating field and application of reference wound surfaces. After that, the future wound was marked on the skin (1.5×1.5 cm), the skin was fixed, and the skin with the underlying tissues to the fascia was excised. After that, the future wound was marked on the skin (1.5×1.5 cm), the skin was fixed, and the skin with the underlying tissues to the fascia was excised. After inflicting a wound on animals of all groups, 0.2 ml of a microbial mixture including S. Aureus and P. Аureginosa was injected into the wound area and a waiting period of 30 seconds was maintained. The first group of animals (control) was not treated; The II group of animals was treated from the 2nd day by a single intramuscular injection of 0.12 mg of blastomunil; The III group of animals was treated by local application of 0.12 mg of blastomunil to the wound with application of a sterile gauze bandage; The IV group of animals was treated by local application of 0.02% decamethoxine (decasan) with application of a sterile gauze bandage; The fifth group of animals was treated by local application of 0.02% decamethoxine in combination with blastomunil and also by applying a sterile gauze bandage; The VI group of animals was treated by a single intramuscular injection of 0.12 mg of blastomunil at the beginning of the experiment, followed by daily local treatment of a combination of 0.02% decamethoxine and blastomunil and application of a sterile gauze bandage. From the 1st to the 14th day, medical measures were carried out, T0 of the body was measured, the condition of the wound was photographed with subsequent analysis, the behavior of the animals was studied, and blood sampling was carried out.
The results. The area of the wounds from the first day to the 14th decreased in all groups, however, the animals of the first group felt worse during the 1-5 days than the animals of the other groups. They showed hypodynamia, decreased response to sounds and light, as well as suppressed appetite. In animals of 2-6 groups, in general, the appetite was good, and the behavior of the rats in the cages was lively. can be seen, Treatment of wounds with topical blastomunil 2.75 times faster wound healing compared to the control group. "Blastomunil" also showed an immunostimulating effect.
Conclusion. Thus, a positive effect of the immunomodulator "Blastomunil" on the wound process was established, showing an immunomodulatory effect and a 2.75-fold acceleration of wound healing compared to the control group of animals.