bluuebunny/crossref_metadata_2025 huggingface dataset:
AN ELECTRONMICROSCOPICAL STUDY ON PEROXIDASE ACTIVITY OF RABBIT MONOCYTES, RESIDENT AND EXUDATE MACROPHAGES
Authors: Oka Kuniyuki , Miyazaki Mutsuko , Kojima Mizu
Published: December 2008

Changes of peroxidase (PO) activity in blood monocytes, untreated or stimulated peritoneal macrophages and subcutaneous exudate macrophages of rabbits were investigated with the glucose‐oxidase method in the culture experiments of these cells after their in vitro or in vivo adherence to the surface of foreign bodies. 1) PO activity was demonstrated in the nuclear envelope (NE) and rough endoplasmic reticulum (rER) in 75% of the untreated peritoneal macrophages. 2) Blood monocytes, most of the stimulated peritoneal macrophages, and subcutaneous exudate macrophages showed no PO positivity. 3) In the in vitro culture of blood monocytes and stimulated peritoneal macrophages, there appeared from 2 hours cells showing PO positivity in NE and rER. 4) Although blood monocytes, stimulated peritoneal macrophages, and subcutaneous exudate macrophages after their in vivo surface adherence showed no PO positivity, it appeared in these cells when further cultured in vitro. From the fact that such a positive change of PO activity in these cultured cells is not only a transient phenomenon but also in vitro cultural one, we cannot subscribe to the view that blood monocyte is the precursor of resident macrophages. ACTA PATHOL. JPN. 32: 445∼460, 1982.
ANTIGENIC ASSOCIATION BETWEEN KIDNEY AND THYMOCYTE
Authors: Harada Keiko , Yamamoto Tadashi , Hara Masanori , Kihara Itaru
Published: December 2008

Commonly shared antigens between the renal tissue and lymphocytes, especially the thymocyte were studied In rats by immunofluorescent technique. To clarify the specificity of antisera, absorption analysis was carried out. The brain‐associated thymocyte antigen, presumably Thy 1 antigen, was detected by anti‐rat thymocyte serum (ATS) or anti‐rat brain serum (ABS) not only on the surface of thymocytes but also in renal glomeruli. The distribution or localization of the antigen in glomeruli was considerably different between acetone‐fixed and unfixed kidney sections. On the fixed section the antigen was shown exclusively in the glomerular mesangium. However, it appeared to be diffusely dispersed on glomerular tissue on the unfixed section. On the other hand, antisera against kidney‐related antigens, anti‐Fx1A, anti‐GBM, and anti‐CIG, did not react with the surface of thymocytes, or they were not absorbed with brain homogenate. ACTA PATHOL. JPN. 32: 483∼489, 1982.
SYSTEMIC LUPUS ERYTHEMATOSUS ASSOCIATED WITH MULTIPLE NODULAR HYPERPLASIA OF THE LIVER
Authors: Kuramochi Shigeru , Tashiro Yukio , Torikata Chikao , Watanabe Yonosuke
Published: December 2008

Three autospy cases of systemic lupus erythematosus with uinique association of multiple nodular hyperplasia (MNH) of the liver, portal hypertension, and hypertensive pulmonary vascular disease are reported. None of the patients had received oral contraceptive or androgenic steroid, but they were treated with glucocorticoids for 2 to 11 years. Raynaud's phenomenon, sclerodactyly, and mild impairment of the kidney were the common clinical features. Macroscopically, MNH is characterized by many nodules scattered throughout the non‐cirrhotic liver, and histologically, each nodule is made up of normal‐appearing hepatocytes and not encapsulated. Portal tracts are scanty in the nodules. MNH seems to be a regenerative‐hyperplastic process, but its true nature still remains unclear. Relationships between MNH and portal hypertension, MNH and pulmonary hypertension, and collagen disease and pulmonary hypertension are discussed. A brief review of the literature concerning multiple benign hepatocellular tumors similar to MNH is also presented. ACTA PATHOL. JPN. 32: 547∼560, 1982.
IMMUNOHISTOCHEMICAL AND ELECTRON MICROSCOPIC STUDIES ON HASHIMOTO'S THYROIDITIS
Authors: Matsuta Morimasa
Published: December 2008

The thyroid glands of nine patients with Hashimoto's thyroiditis were studied by immunoperoxidase method for immunoglobulin (Ig) and thyroid hormone, and of these four tissue specimens were further examined by electron microscope. Immunoperoxidase method for Ig revealed that about 63% of the infiltrating cells contained Ig, and that about 90% of such Ig‐containing cells had IgG. IgG‐containing cells seemed to secrete autoantibodies. Immunoperoxidase method for thyroid hormone and electron microscopy revealed that there was a good correlation between the morphological features of the thyroid follicle and its immunohistochemical staining pattern. In follicles composed of columnar cells, the colloid and cytoplasm of some epithelial cells were immunohistochemically stained. Dense deposits, regarded as immune complexes, were observed in the basement membrane of these follicles, and lymphocytes were seen between the adjacent cells. When similar deposits appeared in the basement membrane of such follicles, 4 or more lymphocytes per follicle could be seen among the epithelial cells. The present findings seem to indicate that antibody‐dependent cell‐mediated cytotoxicity plays an important role in the pathogenesis of Hashimoto's thyroiditis.
PALE EOSINOPHILIC INCLUSIONS SIMULATING GROUND‐GLASS APPEARANCE OF CELLS OF HEPATOCELLULAR CARCINOMA
Authors: Nakanuma Yasuni , Kono Naoko , Ohta Goroku , Shirasaki Shinji , Takeshita Haruo , Watanabe Kunishige , Tsuda Shoji , Yoshizawa Hiroshi
Published: December 2008

Pale eosinophilic inclusions simulating ground‐glass appearance were observed in cells of hepatocellular carcinoma. They were round or elliptical in shape, and their size was about 14 μm in diameter. Light eosinophilia and fine granulation with homogeneous appearance were delineated from other cellular materials. They were negative for HBsAg blood plasma proteins including α1‐antitrypsin, and α‐fetoprotein by immunohistochemical stainings. Ultrastructurally, they were well‐demarcated and consisted of homogeneous finely granular matrix. Histological, histochemical and ultrastructural features were different from several intracellular inclusions hitherto reported on hepatocellular carcinoma. These changes may represent a deposition of secretory proteinous materials in cells of hepatocellular carcinoma, but their chemical and antigenetical content could not be identified.
LANGERHANS CELLS AMONG BILE DUCT EPITHELIAL CELLS IN CHRONIC LIVER DISEASE
Authors: Tobe Kazuo , Endo Hiroshi , Nagashima Hideo , Kobayashi Toshinari
Published: December 2008

Langerhans cells were found among bile duct epithelial cells in a biopsy specimen from a patient with chronic liver disease showing cholangitic features. The bile duct was 90 μm In diameter and surrounded with mononuclear cell infiltration. Under the electron microscope, the cell had a clear cytoplasm and contained a deeply indented nucleus, a centriole, well‐developed Golgi complexes and many rod‐shaped bodies (Birbeck granules).
CHONDROMYXOID FIBROMA OF BONE
Authors: Ushigome Shin'ichiro , Takakuwa Toshifumi , Shinagawa Toshihito , Kishida Hidemi , Yamazaki Makoto
Published: December 2008

A resected case of chondromyxoid fibroma of the right fibula in a 31‐year‐old male is presented. The histology was composed of characteristic lobular features of this tumor with abundant cartilaginous matrix. The ultrastructural study exhibited stellate, ovoid or elongated tumor cells with features of cartilage cells and abundant loose matrix with many fine filamentous structures. The nuclei were often of peculiarly indented contour and revealed thick fibrous lamina. The villous cell processes and intracytoplasmic fine filaments were prominent. No cells suggesting a fibroblast were recognized in this study. From the light microscopical and ultrastructural findings of this case, it is supported that this tumor might be derived from adult cartilage cells most likely related to the epiphyseal cartilage rather than fibroblasts.
DIROFILARIAL INFECTION IN HUMAN LUNGS
Authors: Tsukayama Chotatsu , Manabe Toshiaki , Miura Yoh
Published: December 2008

Two cases of dirofilarial infection in the human being were reported. Dirofilarial infection is common in dogs but rather rare in human. A review of Japanese literature disclosed 13 cases of human dirofilarial infection. Scarcity of symptoms and its morphological similarity to tuberculosis or infarction as well as the popularity in pets suggest that the true incidence of infection may actually be higher than the above figure. Our cases alert us for the recognition of human dirofilarial infection, and careful examination of multiple sections in granulomatous or nonspecific infarcted lesions is suggested.
PACHYMENINGITIS CERVICALIS HYPERTROPHICA
Authors: Oonishi Tadahiro , Ishiko Toshitaka , Arai Masanobu , Kase Masao , Matsushita Masaaki
Published: December 2008

Presented here is an autopsy case of pachymeningitis cervicalis hypertrophica. A 77‐year‐old female had the initial symptoms of cervical radiculopathy which terminated in the syndrome of spinal cord transection. Prior to the onset of radiculopathy, the patient had been admitted to the hospital because of a fever and had been shown to have granulomatous sinusitis. Autopsy revealed chronic granulomatous pachymeningitis of the cervical spinal cord, the lesion being mostly restricted to this region. From histopathologic similarities, the extension of sinusitis to cervical pachymeningitis was assumed, but this was not confirmed. An attempt to discover the causative micro‐organisms was unsuccessful. The possibility of a limited form of Wegener's granulomatosis could be excluded in view of the absence of true angiitis. Corresponding to the levels of pachymeningitis, the spinal cord was also severely damaged, which was principally due to circulatory disturbance as a result of pachymeningitis.
POSTIRRADIATION SARCOMA (MALIGNANT FIBROUS HISTIOCYTOMA) IN THE INGUINAL REGION
Authors: Tanaka Toshio , Miwa Hiroaki , Orita Kunzo , Hiraki Yoshio , Yamamoto Michio , Hashimoto Kimio , Yumoto Tokichi
Published: December 2008

A female patient who died of apparent postirradiation sarcoma at the age of 39 was reported. Following a traffic accident, she developed squamous cell carcinoma at the injured right heel 20 years later, and also metastasis to both inguinal and popliteal lymph nodes. During the period from September 1966 to May 1968, she had received Co60 irradiation to the right inguinal and para‐aortic areas, 9,600 and 6,600 Roentgen, respectively. Ten years later, she manifested a huge tumor in the inguinal region which extended further to the retroperitoneum; light and electron microscopic examinations proved to be a malignant fibrous histiocytoma. She died of massive pulmonary metastasis two years later. Histological differentiation of the tumor and relationship between irradiation and sarcoma induction were briefly discussed, and the necessity of reevalution of soft part sarcomas was emphasized.
CHRONIC MASUGI NEPHRITIS IN THE RAT
Authors: Kondo Yoichiro , Akikusa Bunshiro
Published: December 2008

Chronic focal glomerulonephritis was induced in the rat by using goat nephrotoxic IgG. In the later stage (6 to 15 months) a number of glomeruli were found to be attached to Bowman's capsule with variable segmental sclerosis and/or hyalinosis. The morphogenesis of the changes was studied by electron microscopy. It was found that partial cytoplasmic necrosis of podocytes was an initiating event of the capsular adhesion. When this occurred in the peripheral tufts, the denuded portion of the glomerular basement membrane (GBM) became attached to the parietal epithelium, resulting in progressive thickening of the mesangium and GBM, deposition of hyaline or proteinaceous material, and desquamation of the endothelial sheet, the features being compatible with segmental glomerular sclerosis and/or hyalinosis. It seemed that these changes, in turn, accelerated podocyte desquamation and extension of the capsular adhesion, producing a progressive cycle that terminated in global glomerular hyalinosis. Such a sequence of events most often occurred in rats with massive proteinuria, suggesting that persistent, severe proteinuria caused the podocyte lesion which led to the development of segmental glomerular sclerosis and/or hyalinosis.
PATHOGENESIS OF 1,2‐DIMETHYLHYDRAZINE‐INDUCED CARCINOMAS IN RAT INTESTINE
Authors: Takemiya Masatake , Miyayama Haruhiko , Takeuchi Tadao
Published: December 2008

Two hundred twenty seven lesions of DMH‐induced intestinal carcinomas of 68 male Wistar rats were investigated by means of histochemical and electron microscopic properties to study their histogenesis and mucinogenesis. Among the epithelial glycoproteins, surface glycoprotein on the microvilli with glycocalyces was distinguishable from mucin in the cytoplasm based on histochemical staining and ultrastructural findings. From the phenotypic characteristics of glycoproteins, it was suggested that DMH‐induced carcinomas could be classified into mucin producing carcinomas composed mainly of cells containing intracellular mucins and non‐mucin producing ones. The mucin producing carcinomas consisted of poorly differentiated adenocarcinoma, signet ring cell carcinoma, and mucinous carcinoma presumably resulting from various degrees of differentiation into goblet cells. Their staining attitude varied and cells with neutral mucin, sialomucin and sulphated mucin were observed in the signet ring cell carcinoma. In addition, abnormal synthesis of glycoproteins was observed with ConA‐paradox staining and PATS/PB/KOH/PAS. These histochemical properties suggested irregulatory synthesis of glycoproteins in carcinomas.

In non‐mucin producing carcinomas, glycoproteins were found mainly on the apical surface and contained sialomucin.

A deviated differentiation into an absorptive cell could form an intracytoplasmic cyst filled with an aggregation of glycocalyces, so becoming another type of signet ring cell.
EXPERIMENTAL INDUCTION OF GLOMERULONEPHRITIS MEDIATED BY ANTI‐GLOMERULAR BASEMENT MEMBRANE AND ANTI‐BRUSH BORDER ANTIBODIES IN A SINGLE RAT
Authors: Hara Masanori
Published: December 2008

This report describes induction of nephritis, which was concurrently mediated by anti‐glomerular basement membrane antibody (antl‐GBM) and anti‐brush border antibody, in the Wistar rat Immunized with a solubilized renal antigen (S‐RA). The antigen was prepared by digestion of rat cortical tissue with trypsin and pronase. Ouchterlony test using antisera to the rat GBM and brush border showed that the S‐RA contained both antigens. From the S‐RA the brush border antigen was Isolated by affinity chromatography. At the 8th week rats injected with the S‐RA showed a linear or combined linear and granular distribution of rat IgG and C3 along the GBM in immuno‐fluourescence. The capillary granular pattern was only observed at the 16th week. In contrast rats injected with the brush border antigen remained in a capillary granular pattern throughout the experimental course. It was suggested that the rat nephritis injected with the S‐RA was mediated by the antibodies capable of reacting with at leat two different antigens, namely the GBM and the brush border. The possibility was confirmed by demonstrating the coexistence of these two kinds of antibodies in the serum and kidney eluate from the nephritic rats.
PROGRESSIVE MULTIFOCAL LEUKOENCEPHALOPATHY
Authors: Nagashima Kazuo , Yamaguchi Kazuyoshi , Nakase Hirobumi , Miyazaki Jun
Published: December 2008

Progressive multifocal leukoencephalopathy developed in a 70‐year‐old man with hepatoma treated with oncostatica. The brain showed multiple demyelinated lesions in the upper half of the cerebral white matter. The lesions consisted of proliferation of hypertrophied astrocytes and glial cells with abnormally large nuclei. These nuclei were shown to be filled with DNA by DAPI staining and reacted specifically with anti‐JC antibody by immunofluorescence. Papova‐like virions were confirmed on both the sections and negatively‐stained brain extract under electron microscopy. The sixteen cases so far reported in Japan were reviewed.
INFLAMMATORY ACTIVITIES OF SYNOVIAL FLUIDS FROM RHEUMATOID‐ AND OSTEO‐ARTHRITIS TO GUINEA PIG SKIN
Authors: Kambara Takeshi , Kutsuna Tatsuo , Okamoto Tsutomu , Nakamura Tadashi
Published: December 2008

For initial study to examine the chemical pathogenesis of rheumatoid and non‐rheumatoid arthritis, the nature and inflammatory activities of 12 synovial fluids from rheumatoid arthritis and 10 from osteoarthritis were studied. Both rheumatoid and non‐rheumatoid synovial fluids were found to have high protein concentration, acid and neutral protease activities. When injected 0.1 ml intradermally to normal guinea pig skin, both synovial fluids induced a strong vascular permeability at 0 minute, mild one at 60 minutes, and mild inflammation which comprised histologically moderate leukocyte infiltration around the venules, swelling of venular endothelial cells, edema, and degeneration of collagen fibrils in the deep dermis of the guinea pig skin 4 hours following the injection. However, these parameters of inflammation induced by synovial fluids from both arthritis were of same degree in intensity. The identification of chemical mediators of inflammation in the synovial fluids of both arthritis requires further study.
A COMPARATIVE ELECTRON MICROSCOPIC STUDY OF LEIOMYOSARCOMA AND LEIOMYOMA OF THE DUODENUM
Authors: Murao Tsuyoshi
Published: December 2008

Comparison of ultrastructural features between leiomyosarcoma and leiomyoma of the duodenum was made. There was no absolute difference between the two, but the following ultrastructural features of leiomyosarcoma were different from leiomyoma: (1) occasional conglomerations of mitochondria surrounded by granular endoplasmic reticulum; (2) quite rare presence of single cilia; (3) frequent occurrence of intranuclear inclusion bodies; (4) variability in nuclear size and shape; and (5) wide interstitial space with scanty collagen fibrils. Although both tumors were considered to be of smooth muscle origin, their ultrastructural features were distinctively different from the normal smooth muscle in some points. In addition to the light microscopic findings, ultrastructural features were considered to be valuable for distinguishing the malignant from the benign smooth muscle tumors in the gastrointestinal tract.
A TUMOR COMPOSED OF MYOFIBROBLASTS An Ultrastructural Study
Authors: Hashimoto Kimio , Matsui Katsuaki , Akeho Masahiro , Okamoto Kimio , Yumoto Tokichi , Endo Akio
Published: December 2008

A subcutaneous tumor of the right temporal region of head was removed from an 8‐year‐old boy. This tumor was studied by light and electron microscopy. He was found to have had a retroperitoneal inflammatory fibrous histiocytoma which had been excised seven years ago. The subcutaneous tumor histologically consisted of spindle cells in fascicles, which did not arrange in a storiform or herring‐bone pattern. Electron microscopically, this tumor was chiefly composed of myofibroblasts. Electron microscopy is essential to determine the cell types of a tumor and we have classified this tumor as a tumor of myofibroblasts. The relationship between the retroperitoneal tumor and the head tumor was discussed.
MALIGNANT HYPERTHERMIA
Authors: Ohmori Masaki , Kobayashi Shoji , Ohtsuki Yuhji , Ogawa Katsuo
Published: December 2008

A report is made of a 65‐year‐old male who died of a malignant hyperthermia of 42°C. Symptoms included muscle rigidity at the termination of operation for neck‐clipping of an aneurysm of the anterior communicating artery. Latent myopathy was observed in skeletal muscle, and a bleeding focus was present in the left anterior region of the hypothalamus, coinciding with the temperature regulation center. The mechanism of onset of this disease is still poorly understood, but it seems that this disease occurred due to synergic effects of the hypothalamic hemorrhage and the pre‐existing myopathy. The serum GPK level at the time of death was abnormally high (250U), and 3.4% of isozyme GPK1 was detected. The serum myoglobin was 204, 850 ng/ml, a markedly high level, and myoglobinuric nephrosis was present as a result.
TUBULAR MYELIN FIGURES IN THE LUNG WITH WILSON‐MIKITY SYNDROME
Authors: Manabe Toshiaki , Miura Yoh
Published: December 2008

An electron microscopic examination of the lung from a patient with Wilson‐Mikity syndrome revealed abundant tubular myelin in the atelectatic areas. The tubular myelin was seen free and adjacent to secreted lamellar bodies in the alveolar spaces as well as within macrophages, but never in type II cells. The transverse dimension of the tubular units ranged from 24 to 30 run, and the distance between two tubules was 5–6 nm. Occasionally the center of a tubule contained a single electron dense particle. The structure of the tubular myelin presented here differs to some degree from that in rat lungs. To our knowledge, the presence of tubular myelin figures has not been reported in cases of Wilson‐Mikity syndrome. Their relation to the etiology of the disease is totally unknown; however, our findings that they were present only in the area of atelectasis but not in emphysematous areas may be significant. An accumulation of similar cases with ultrastructural study and combined biochemical and physiological studies is awaited to permit some interpretation.
MUCOEPIDERMOID CARCINOMA OF THE THYMIC REGION
Authors: Tanaka Michio , Shimokawa Reiko , Matsubara Osamu , Aoki Nozomu , Kamiyama Ryuichi , Kasuga Tsutomu , Hatakeyama Shigeru
Published: December 2008

A case of mucoepidermold carcinoma in thymus in a 59‐year‐old Japanese female is presented. She died of cardiac tamponade due to tumor invasion after a 5 years clinical course. At autopsy the main tumor was found in the thymic region with metastases to the sternum, regional lymph nodes, pericardial, and left pleural cavity. The mucoepidermold carcinoma might be probably originated from a hen's egg‐sized cyst which was located in the upper posterior aspect of the tumor‐Involved thymus. No teratomatous components were present. The cyst was most likely to be of thymic or bronchogenic cyst origin, though it was not determined, in view of the lining with pseudo‐stratified ciliated columnar epithelium of the cystic wall and the surrounding with the thymic tissue outside. Moreover, there was thymic hyperplasia with germinal center that was compatible with SLE‐like symptoms in her past history and autoimmune nature of the autopsy findings of pulmonary fibrosis.
PITUITARY–ADRENAL AXIS IN PINEALOMA
Authors: Hasegawa Akio , Mori Wataru
Published: December 2008

Autopsy series of 19 patients with pineal and anterior third ventricle (suprasellar) tumors were analyzed with regard to shift in pituitary‐adrenal axis. Germinomas formed the most common subgroup in this series (79%). Metastatic tumor growth in the pituitary gland caused adrenal atrophy In 4 cases. Some patients with pineal neoplasms had larger adrenals in weight compared with age‐ and sex‐matched controls of Japanese. In three patients with sexual precocity, adrenal weight was larger. It is concluded that clinical importance exists in anterior pituitary insufficiency with adrenal atrophy, but hyperfunction of the hypophyseoadrenal system might occur during the course of the disease.
LIPOSARCOMA
Authors: Hashimoto Hiroshi , Enjoji Munetomo
Published: December 2008

Fifty‐two cases of liposarcoma were analyzed clinicopathologically, and were grouped into the following five types: myxoid (28 cases), well‐differentiated (14 cases), pleomorphic (4 cases), round‐cell (3 cases), and mixed (3 cases). Three of the 28 myxoid tumors showed a pleomorphic pattern resembling that of the myxoid variant of malignant fibrous histiocytoma, and one of the 3 round‐cell tumors was considered to be a malignant counterpart of hibernoma. Ages ranged from 22 to 86 years with the average age of 49 years, the average patients with myxoid or round‐cell liposarcoma being over 10 years younger than those with well‐differentiated or pleomorphic liposarcoma. There was also a variation in the site of predilection of the cases: the great majority of myxoid type tumors occurred in the thigh, popliteal fossa and buttock (23 cases), while the majority of well‐differentiated type tumors were seen in the retroperitoneum (10 cases). The rate of local recurrence of the tumor was 46%. Four of the 5 well‐differentiated liposarcomas which recurred exhibited dedifferentiated pleomorphic components resembling those in malignant fibrous histiocytoma. The prognosis was more favorable in patients with ordinary myxoid tumors than in those with well‐differentiated tumors. The overall relative five‐year survival rate was 63%.
ARGYROPHIL REACTIVE CELLS IN THE NORMAL UTERUS AND DIFFERENTIATED ENDOMETRIAL ADENOCARCINOMA
Authors: Satake Tatsunari , Matsuyama Mutsushi , Kuzuya Kazuo , Suchi Taizan , Sato Tsuneko
Published: December 2008

Argyrophil cells were seen in 2 of 134 cervical tissue blocks obtained from 18 uteri, but none in the endometrium. In 10 of 30 cases of differentiated endometrial adenocarcinoma cells contained argyrophil granules stained with Grimelius method. These argyrophil reactive glandular cells gave positive d‐PAS and Alcian blue (pH 2.5, PH 1.0) reactions, but gave no argyrophil reaction with PAS‐Grimelius double staining method. It was also demonstrated that these glandular cells contained sulphomucins, because High iron diamine (HID) method gave positive reaction with HID‐Alcian blue pH 2.5 double staining. Dense granules, which were submicroscopically seen in cells of well differentiated endometrial adenocarcinomas, were diagnosed as exocrine secretory granules, because of their localization in the cytoplasm and distinct morphological transformation from condensed vacuoles into mature secretory granules. From these findings, argyrophil granules which were seen in cells of the differentiated endometrial adenocarcinomas were thought not to correspond to endocrine granules but mucosubstances.
ULTRASTRUCTURE OF INTRACYTOPLASMIC INCLUSION BODIES IN HEPATOCYTES OF GRISEOFULVIN‐TREATED MICE
Authors: Matsumoto Kazunori , Ohta Shinichi , Murao Satoru , Shiga Junji
Published: December 2008

Intracytoplasmic eosinophilic inclusion bodies light‐microscopically identical to human alcoholic hyalin were produced in hepatocytes of griseofulvin‐treated mice. Electron‐microscopic investigation on these inclusion bodies revealed two morphologically different fibrils. The most frequently observed fibrils in liver tissues appeared straight or slightly curved in longitudinal sections and granular in cross sections. High‐power electron micrographs on these fibrils revealed that each fibril consisted of parallel‐running fine filaments of about 1.25 nm thick which were separated by spaces of about 1.25 nm. The ultrastructural appearance of these fibrils resembled that of myelin sheath in longitudinal sections. They usually measured from 13 to 32 nm wide and up to 0.9 μ long. Fibrils with larger diameter of up to 62 nm were infrequently encountered. Rod‐like fibrils ultrastructurally similar to those seen in human alcoholic hyalin were also found in isolated materials. All these fibrils were isolated in the same fraction by a slight modification of a discontinuous sucrose gradient ultracentrifugation and a two phase polymer centrifugation. The present observtion suggests that Intracytoplasmic inclusion bodies produced in this animal model are composed of morphologically different fibrils. These different fibrils could share the same origin.
ANOMALOUS ORIGIN OF THE LEFT CORONARY ARTERY
Authors: Murai Tatsuya , Kawaguchi Yukihiro , Inui Michio
Published: December 2008

Two autopsy cases of anomalous coronry artery were reported. The patients were a schoolgirl and a schoolboy, who died suddenly during physical exercise at the age of twelve. At postmortem examination in both cases the left coronary artery arose at the comissure from aorta, and in one case the left coronary ostium was completely obstructed with fibrous tissue, whereas in the other case it was severely stenotic. The cause of anomalous ostial position should be regarded as congenital, but the complete occlusion and severe stenosis at the orifice seemed to be modified by several factors ‐ both congenital acquired. For the prevention of sudden death caused by this anomaly, cardiac angiography would have been necessary at the outset of anginal pain.
SUBACUTE SCLEROSING PANENCEPHALITIS
Authors: Ueda Keishi , Imamura Takahisa , Kawaguchi Tetsu , Tamari Hideo , Kambara Takeshi
Published: December 2008

An autopsy case of subacute sclerosing panencephalitis (SSPE) in a 5‐year‐old boy, with rapid progression to a comatose state in 2 weeks after the onset of right hemiplegia, is described. The levels of antibody to measles virus in the serum and the cerebrospinal fluid were increased, and high levels of IgG in the latter were found. A characteristic pattern of electroencephalogram (EEG) showing periodic suppression of high voltage complexs was also found during the course of the disease. Microscopical examination revealed perivascular cuffing, numerous hypertrophied astrocytes with a diffuse gliosis and sporadic intranuclear inclusions in the brain. In addition to these typical findings of SSPE, impaired cellular immunity was recognized by delayed skin test in vivo, and pathologically severe atrophy of thymus, and follicular atrophy of spleen with amyloid deposition in the wall of the sheathed arteries were found.
HISTOLOGICAL CLASSIFICATION OF RESORBED BONE
Authors: Urago Atsushi
Published: December 2008

A histological classification of resorbed bone is reported in this paper. The materials used were 426 human bones in autopsy and surgical cases, ranging from 3‐month‐old fetus to 95‐year‐old. The resorbed bones were divided into osteoclasis (osteoclastic resorption) and osteolysis (non‐osteoclastic resorption). Osteoclasis was observed in the cases of development, growth, active stage of granulation tissue, and inflammation, and some cases of malignant tumor invasion. Osteolysis was observed in the cases of senile atrophy, osteoporosis, ischemia, radiation damage, hormonal and chemical effects, non‐active stage of granulation tissue and inflammation, and some cases of malignant tumor invasion. Based on these findings, human resorbed bones can be classified into three histological types: rapid, slow, and static. Such a classification could be certified through the results of animal experiments. A possibility exists that osteoclasis occurs in rapid resorption and osteolysis is in slow and static resorption. Furthermore, it is suggested that osteoclasis is passive resorption and osteolysis is autolysis.
MEDULLARY CARCINOMA OF THE THYROID WITH ECTOPIC ACTH SYNDROME
Authors: Kakudo Kenichi , Miyauchi Akira , Ogihara Toshio , Takai Shin‐ichiro , Kitamura Hajime , Kumahara Yuichi , Kawaoi Akira
Published: December 2008

An autopsied patient with medullary carcinoma of the thyroid and ectopic ACTH syndrome is reported. A microadenoma of pancreatic islet coexisted in this case, which is assumed to be of D cell origin. Immunohistochemical study revealed decreased number of pituitary ACTH cells. Some of them showed Crooke's degeneration. Hormone assay study of tumor tissue and plasma disclosed abnormal ACTH, β‐MSH as well as calcitonin. Somatostatin and Substance P were also demonstrated in tissue. Histologically the tumor showed solid alveolar pattern with a minor part consisting of small cell variant and this histologic variation is discussed.
IgM‐MONOCLONAL GAMMOPATHY ASSOCIATED WITH MALIGNANT LYMPHOMA WITH RECURRENT PLEURAL EFFUSION
Authors: Tada Toyohiro , Wakabayashi Takashi , Nakamura Takaaki , Kishimoto Hidemasa
Published: December 2008

A case of malignant lymphoma (plasmacytold lymphocytic type) with IgM‐monoclonal gammopathy is presented. The main site of the lesion was in the lung. The sole clinical manifestation was pleuropulmonary involvement with massive pleural effusion. A clue to the diagnosis was given by cytologlcal and immunocytological examination of pleural aspirates. Subsequent immunological survey of serum protein and a bronchial biopsy confirmed the diagnosis.
PRIMARY NODULAR LYMPHOMA OF THE LUNG Immunoperoxidase and Ultrastructural Studies
Authors: Medeiros L. Jeffrey , Shimizu Masaki , Legg Merle , Seidman Joel , Katayama Isao
Published: December 2008

In two patients, primary nodular lymphomas of the lung which presented incidentally were diagnosed following routine chest radiography, exploratory thoracotomy, and the application of histologic criteria. The diagnoses were confirmed using the peroxidase‐antiperoxidase (PAP) technique and transmission electron microscopy. Whereas most investigators had demonstrated intracytoplasmic immunoglobulins in only the large cells of nodular lymphomas using the standard PAP method, we were able to prove the presence of monoclonal immunoglobulin within the cytoplasm of both the large cells and small cells using a modification of the PAP technique as described by DeLellis. This modification uses very dilute antisera and a prolonged incubation time and is more highly sensitive and specific. Monoclonal immunoglobulin was also shown to be within the cytoplasm of mature plasma cells observed within the interfollicular areas. This monoclonal immunoglobulin was the same as that seen within the cytoplasm of the small and large cells of each respective lymphoma. Electron microscopy permitted the identification of three cell types within each patient's tumor: small cells, large cells, and dendritic reticulum cells((DRCs). The finding of the DRCs, presently considered a residual marker of the germinal center, is supporting evidence that both lymphomas studied were nodular lymphomas of B cell derivation.
MALIGNANT MESENCHYMOMA OF THE HEART
Authors: Tanaka Takuji , Bunai Yasuo , Nishikawa Akiyoshi , Kawai Toshiro , Mori Hideki , Takahashi Masayoshi
Published: December 2008

A case of a rare primary cardiac tumor in a 46‐year‐old woman is described. The tumor arose from the left atrium and was histologically composed of multiple mesenchymal elements of fibrosarcoma, rhabdomyosarcoma, chondrosarcoma, myxosarcoma, and liposarcoma. Metastasis of this tumor occurred in the left femur, lung, and hilar lymph nodes after the second heart operation. Histogenesis of malignant mesenchyoma was considered with a survey of the literature.
ANNULAR ULCER OF THE STAGNANT ILEUM
Authors: SAITO Ken , SHIMIZU Hideo , YOKOYAMA Takeshi , KAWATA Katsuya , MATSUMUBA Takanori , MOBIOKA Yasuhiko
Published: December 2008

Four cases of chronic annular ulcers of the stagnant ileum were described, The ulcers developed at the necks of the distended oral blind loops formed by side–to–side intestinal anastomoses in 3 cases and orally to the site of ileal stenosis by a congenital mesodiverticular band in one case. All ulcers were shallow and characterized by desmoplastic reaction and extensive veno–occlusive lesions. Arterio–occlusive lesions were sparse except for the youngest case. Intestinal ischemia caused by transient or recurrent ileal intussusception is the possible cause of these ulcers. ACTA PATHOL. JPN. 33: 257–263, 1983.
ULTRASTRUCTURAL CHARACTERISTICS OF DUODENAL SOMATOSTATINOMAS
Authors: KANEKO Hiroshi , SUMIDA TosMko , TOSHIMA Muneatsu , KOBAYASHI Hiroshi , KITAZAWA Mikio
Published: December 2008

Ultrastructural findings of granules and vesicles appearing in tumor cells of somatostatinoma are reported. Except for D granules, one granule and four kinds of vesicles were discerned. Some tumor cells had granules of the exocrine type which were similar to those of the chief cells of the stomach. Very small clear vesicles (YO and KO) had accumulated mainly in the apical region of the somatostatinoma cells. Both vesicles were round, unit–membrane bounded and clear in the center, each measuring about 90 and 30 nm in diameter. The cored vesicles normally seen in the sympathetic system were revealed. Doughnut vesicles characterized by double contour membranes were detected together with the cored vesicles.

A mixutre of exocrine cells of the chief cell–type in the paraneuroma has not been previously reported. And we have as yet very little information as to the doughnut vesicle. ACTA PATHOL. JPN. 33: 359–366, 1983.
ULTRASTRUCTURAL STUDY OF Ni3S2‐INDUCED TUMORS IN RATS
Authors: Yamashiro Shigeto , Basrur Parvathi K. , Gilman John P.W. , Hulland Thomas J. , Fujimoto Yutaka
Published: December 2008

An ultrastructural study was conducted on a total of 24 tumors selected from 70 tumors Induced by intramuscular injection of Ni3S2 in Fischer and Hooded rats. Well differentiated rhabdomyosarcomas were predominantly composed of strap‐like cells containing numerous myofilaments occasionally forming S‐line‐like structures, whereas poorly differentiated rhabdomyosarcomas were made up mainly of round or oval cells with abundant cytoplasm. Embryonal rhabdomyosarcomas contained cells resembling the mast cells in myxomatous areas as well as spindle‐shaped myoblast‐like cells. Mesenchymal tumors exhibited morphological characteristics of the lymphoid cells. The tumor cells from leiomyosarcoma‐like tumors showed myoblast‐like appearance. Tumors diagnosed as fibrosarcoma‐like tumors at the light microscopic level demonstrated the ultrastructural characteristics of fibrosarcomas. The possibility of a multicentric origin of the tumor cells in Ni3S2‐induced tumors has been discussed.
BASAL CELLS OF THE HUMAN BRONCHIOLE
Authors: Tamai Seiichi
Published: December 2008

Basal cells of human bronchioles were studied by light and electron microscopic histochemistry, and compared with basal cells of major bronchi. Bronchiolar basal cells were found isolated or in isolated groups of a few linked cells. On the other hand, the basal cells of the major bronchi form virtually continuous layers covering the basement membrane. Bronchiolar basal cells were different in ultrastructure and alkaline phosphatase (ALP‐ase) activity from basal cells of major bronchi. Bronchiolar basal cells showed intense ALP‐ase activity on the plasma membrane, but basal cells of the major bronchi showed no activity. Basal cells of bronchioles were flattened, triangular or trapezoid in shape, and showed different organelle features from those of basal cells of major bronchi. ALP‐ase positive bronchiolar flattened basal cells with pinocytoic vesicles were not myoepithelial cells, since actin was not demonstrated by immunohistochemistry. The role of basal cells in the renewal of epithelium appeared to be that of stem cells in both major bronchi and bronchioles, since basal cells of bronchioles appeared to differentiate directly to Clara cells, and to become ciliated cells via intermediate cells.
ACUTE MYCOTIC THYROIDITIS
Authors: Kakudo Kennichi , Kanokogi Mototsugu , Mitstsunobu Masao , Sawada Kenji , Uematsu Kunio , Noguchi Tsukasa , Fujita Shigeru
Published: December 2008

An autopsy case of acute thyroiditis in acute lymphocytic leukemia is presented. The thyroiditis was mycotic and developed severe necrotic inflammation with a hemorrhage and a perithyroidal abscess, and resulting in disseminating mycosis with multiple microabscesses in both kidneys and in myocardium.
CUSHING'S SYNDROME WITH BILATERAL MULTINODULAR ADRENAL HYPERPLASIA
Authors: Takemura Tamiko , Eishi Yoshinobu , Hatakeyama Shigeru , Takahashi Yuji
Published: December 2008

An unusual case of Cushing's syndrome of a 59‐year‐old man with bilateral multinodular adrenal hyperplasia and microadenoma of the pituitary gland is presented. Failure to suppress plasma Cortisol with large doses of dexamethasone may suggest autonomous growth of hyperplastic nodules of the adrenals, which were at first induced by prolonged stimuli of ACTH from the microadenoma of the pituitary gland. ACTH could not be detected in the microadenoma cells on paraffin sections, while Crooke's cells were strongly positive for ACTH. The interrelation between bilateral multinodular adrenal hyperplasia and pituitary microadenoma is discussed.
UNDIFFERENTIATED CARCINOMA OF THE PAROTID GLAND WITH BIZARRE GIANT CELLS
Authors: Hayashi Yoshio , Aoki Nozomu
Published: December 2008

A rare autopsy case of undifferentiated carcinoma of the parotid gland with bizarre giant cells is reported. Grossly, the tumor was fist‐sized, firm, solid, grayish‐white in color, and was not encapsulated, with a bean‐sized ulceration on the skin. Microscopical examination revealed undifferentiated carcinoma of the parotid gland composed of bizarre giant cells, large tumor cells, and spindle cells in part. Ultrastructural examination, recovered from formalin‐fixed material, disclosed filamentous structure in some large tumor cells. It is strongly suggested that myoepithelial cells played an important role in the development of the undifferentiated carcinoma of the parotid gland. The clinical and histopathological characteristics of this case is described, and the literature concerning undifferentiated carcinoma of the parotid gland is reviewed.
SECRETORY COMPONENT and IgA IN ENDOMETRIAL ADENOCARCINOMAS
Authors: Takeda Akihiro , Matsuyama Mutsushi , Kuzuya Kazuo , Chihara Tsutomu , Ariyoshi Yutaka , Suchi Taizan , Kato Kanefusa
Published: December 2008

The localization of secretory component (SG) and IgA was immunohisto‐chemically studied in 6 normal endometrium and 55 endometrial adenocarcinomas including 34 well, 11 moderately and 10 poorly differentiated ones. In normal endometrium, SC localization was found in the cytoplasm of epithelial cells and luminal contents of the gland. IgA showed similar localization of SC. Secretory phase endometrium contained proportionally larger numbers of positive cells for SC and IgA than proliferative phase endometrium. SC localization was found in all cases of well and moderately differentiated carcinomas, while it was found only in 4 cases out of 10 poorly differentiated carcinomas. IgA localization was similar to that of SC and this condition was thought to reveal the binding of IgA to SC existing in the tumor cells. The present immunohistochemical study revealed that the staining intensity of SC well correlated with the histological grade of differentiation of the tumors.
IMMUNOPATHOLOGICAL CHARACTERIZATION OF THE DELAYED HYPERSENSITIVITY SKIN REACTION TO CARRIER PROTEIN
Authors: Yamamoto Tetsuro , Tsueuta Junji , Yasaka Tatsuomi , Kambara Takeshi
Published: December 2008

Characteristics of the allergic skin reaction to free carrier protein in sensitized guinea pig with hapten‐carrier conjugate have been studied. The animals sensitized with a low dose (4 μg) of dinitrophenylated bovine gamma globulin with Freund's complete adjuvant demonstrated a dermatitis when challenged intradermally with a small dose (10 μg) of bovine gamma globulin en the 10th or 14th day. The dermatitis was grossly and histologically similar to the classical form of delayed‐type hypersensitivity skin reaction except lesser induration and less abundant neutrophil infiltration in the former. This reaction was clearly different from cutaneous basophil hypersensitivity because of the long lasting time course and fewer basophil infiltration in the former. No humoral antibody to carrier protein was detected in the sera of the sensitized animals by passive cutaneous anaphylaxis or by immunodiffusion in agar plates, and the state of the hypersensitivity could be passively transferred with peritoneal exudate cells and spleen cells. These observations indicated that the skin reaction to carrier protein might be of primarily delayed‐type hypersensitivity. The vascular permeability change at the reaction sites was observed as a single phase delayed response and preceded the maximum erythema by about ten hours.
AN ULTRASTRUCTURAL STUDY ON PERIDUCTAL ELASTOSIS IN HUMAN BREAST TUMORS
Authors: Nakanishi Isao , Moriizumi Tetsuji , Ooi Akishi , Oda Yoshio , Kajikawa Kinichiro
Published: December 2008

An ultrastructural study on elastosis of human breast tumors was made with special attention to the periductal elastosis and the cell responsible for elastic fiber formation. The elastosis was found prominently in scirrhous type of duct carcinoma. In the area of mild periductal elastosis, the elastic fibers with many microfibrils and a tiny central elastin were seen around the periductal fibroblasts which were characterized by attenuated cytoplasms with aggregates of microfilaments and slightly developed rough endoplasmic reticulum. With the thickening of the periductal wall, such an area was replaced by abundant mature elastic fibers with peripheral microfibrils and a few intervening ordinary fibroblasts. Therefore, it was suggested that the periductal fibroblasts which transformed into ordinary fibroblasts during the development of elastosis were primarily concerned with the elastic fiber formation. In the interlobular tissue in which both fibroblasts and myofibroblasts were present, the elastic fibers were larger than those of the periductal area and had less microfibrils in their periphery. The relationship between microfibrils and elastin during the early elastosis, maturation process of the elastic fibers, and cell modulation of the fibroblasts in the breast elastosis were discussed.
ANAPLASTIC CARCINOMA OF THE ESOPHAGUS
Authors: Tanoue Shokan , Shimoda Tadakazu , Suzuki Masafumi , Ikegami Masahiro , Ishikawa Eisei , Sano Toshiaki
Published: December 2008

The present report includes the histological, electron microscopical and immunohistochemical findings observed in two cases of anaplastic carcinoma of the esophagus. Anaplastic carcinoma of the eosphagus revealed histological features similar to oat‐cell carcinoma and carcinoid. Positive argyrophil reaction, neurosecretory granules, and hormonal activity suggest that both cases come under the category of neuroendocrine cell tumors. Anaplastic carcinoma of the esophagus may be considered as a pluripotential tumor. The biological behavior of this tumor is quite different from that of carcinoid and is a highly malignant tumor.
ELECTRON MICROSCOPIC OBSERVATIONS OF LYMPHANGIOSARCOMA ARISING FROM CHRONIC LYMPHEDEMA
Authors: Yokoyama Shigeo , Nakayama Iwao , Tsuji Kouiohi , Nakamura Toshitaka , Moriuchi Akira , Yamashita Hiroto
Published: December 2008

Lymphangiosarcoma so‐called Stewart‐Treves syndrome, originating from the edematous left leg after hysterectomy in a 53‐year‐old woman has been studied by light and electron microcopy. Histologically the tumor was composed of either vascular channels or solid nests of the neoplastic cells having a large hyperchromatic nucleus with a single or multiple prominent nucleoli. The inner surface of the vascular channels was lined by either single or several layers of identical cells. Erythrocytes were occasionally observed in the lumina of the vascular channels. By electron microscopy the maturation of the neoplastic cells differed considerably from one area to another even in the same section. The immature cells were clustered in groups and consisted of scanty cytoplasm and a large nucleus with prominent nucleoli, whereas some of the mature cells lining the lumina had rod‐shaped tubulated bodies (Weibel‐Palade bodies). Desmosomes were frequently observed between the neoplastic cells. Basal lamina was generally not continuous or not present although some vascular structures were completely surrounded by a basal lamina. An autopsy was performed but revealed no metastatic lesions in any organs or lymph nodes.
ACIDOPHILIC CRYSTALLINE INCLUSION BODY IN THE CAUDATE NERVE CELLS
Authors: Nonaka Hiroko
Published: December 2008

Acidophilic crystalline inclusion bodies (AGBs) within the cytoplasm of large neurons of the caudate nucleus and putamen were described. ACB was eosinophilic needle‐shaped intracytoplasmic material and electronmicros‐copically composed of filamentous material. Some were found in the cisterns of rough‐surfaced endoplasmic reticulum. There was no histochemical and electronmicroscopic similarity between ACB and Mallory's hyaline bodies of hepatocytes. Though ACBs were noted in a high incidence in alcohol drinkers (65.7%), particularly suffering of alcoholic encephalopathy (72.2%), they were also found in patients without drinking habit (53.9%). In the latter cases, the incidence was high in aged people and patients dying of debilitating diseases (75%) or malignant tumors (57.1%). Consequently, ACB is not pathognomonic for chronic alcoholisms. On histochemical and electron microscopic studies, ACB was considered as a proteinic material, originated from rough‐surfaced endoplasmic reticulum, and resulted from some disordered protein synthesis of the neurons.