bluuebunny/crossref_metadata_2025 huggingface dataset:
Empyema Necessitatis Caused by Prevotella melaninogenica and Dialister pneumosintes Resolved with Vacuum-Assisted Closure System: A Case Report
Authors: Martínez Castrejón Esteban Bladimir , Reina-Bautista Erika , Ventura-Gómez Sandra Tania , Maldonado Cisneros Araceli , Juárez Ramos Jessica Alejandra , Durán Miguel Alejandro Sánchez , Aguilar Ventura Jesús , Valencia-Ledezma Omar Esteban , Frías-De-León María Guadalupe , García Salazar Eduardo , Castro-Fuentes Carlos Alberto
Published: September 2024

Empyema necessitatis is a rare complication of an untreated or inadequately controlled empyema. We present the case of an 11-year-old female adolescent living in precarious conditions, overcrowding, incomplete vaccinations, irregular dental hygiene, and no significant family or personal medical history. The patient started with symptoms one week prior to her hospitalization, presenting a persistent sporadic dry cough, and was later diagnosed with complicated pneumonia, resulting in the placement of an endopleural tube. Vancomycin (40 mg/kg/day) and ceftriaxone (75 mg/kg/day) were administered. However, the clinical evolution was unfavorable, with fever and respiratory distress, so a right jugular catheter was placed. The CT scan showed a loculated collection that occupied the entire right lung parenchyma and pneumothorax at the right upper lobe level. After four days of treatment, the patient still presented purulent drainage with persistent right pleural effusion syndrome. P. melaninogenica and D. pneumosintes were identified from the purulent collection on the upper right lobe, so the antimicrobial treatment was adapted to a glycopeptide, Teicoplanin, at a weight-based dosing of 6 mg/kg/day and Metronidazole at a weight-based dosing of 30 mg/kg/day. In addition, VAC therapy was used for 26 days with favorable resolution.
Evaluation of Bacillus velezensis F9 for Cucumber Growth Promotion and Suppression of Fusarium wilt Disease
Authors: Ta Yongquan , Fu Shaowei , Liu Hui , Zhang Caiyun , He Mengru , Yu Hang , Ren Yihua , Han Yunfei , Hu Wenqiong , Yan Zhiqiang , Wang Yonghong
Published: September 2024

Cucumber wilt, caused by Fusarium oxysporum f. sp. cucumerinum (FOC), is a soilborne disease that poses a significant threat to cucumber production, resulting in substantial yield losses. This study aimed to evaluate the biocontrol and growth-promoting effects of Bacillus velezensis, a highly active bacterial strain. In vitro assays revealed that B. velezensis F9 exhibited broad-spectrum antifungal activity against eight plant pathogenic fungi, with inhibition ratio ranging from 62.66% to 88.18%. Additionally, the strain displayed the ability to produce IAA (5.97 ± 1.75 µg/mL), fix nitrogen, produce siderophores, and form biofilms. In vitro growth promotion assays demonstrated that different concentrations of B. velezensis F9 significantly promoted cucumber seedling growth. Furthermore, two pot experiments revealed that the strain exhibited biocontrol efficacy against cucumber wilt, with disease control rates ranging from 42.86% to 67.78%. Notably, the strain significantly increased the plant height, fresh weight, and dry weight, with increases ranging from 20.67% to 60.04%, 40.27% to 75.51%, and 22.07% to 52.54%, respectively. Two field trials confirmed the efficacy of B. velezensis F9 in controlling cucumber wilt, with disease control rates of 44.95% and 33.99%, respectively. The strain effectively alleviated the dwarfing and wilting symptoms caused by the pathogen. Compared with the FOC treatment, the F9 + FOC treatment significantly increased the plant height, fresh weight, and dry weight, with increases of 43.85% and 56.28%, 49.49% and 23.70%, and 36.25% and 73.63%, respectively. Enzyme activity assays indicated that inoculation significantly increased SOD activity in cucumber leaves and neutral phosphatase, sucrase, and urease activity in rhizosphere soil. Correlation analysis revealed a negative correlation between the disease index and plant height, fresh weight, dry weight, and peroxidase activity, with correlation coefficients of −0.53, −0.60, −0.38, and −0.45, respectively. These findings suggest that plant height, fresh weight, and dry weight are significantly negatively correlated with the cucumber disease index, highlighting their importance as indicators for evaluating the biocontrol efficacy of B. velezensis F9. In conclusion, B. velezensis F9 is a highly effective plant growth-promoting rhizobacterium with excellent biocontrol potential, showcasing promising applications in agricultural production.
Design, Construction and Finite Element Analysis of a Hexacopter for Precision Agriculture Applications
Authors: Gutierrez-Rivera Miguel Ernesto , Rumbo-Morales Jesse Y. , Ortiz-Torres Gerardo , Gascon-Avalos Jose J. , Sorcia-Vázquez Felipe D. J. , Torres-Cantero Carlos Alberto , Buenabad-Arias Hector M. , Guillen-Escamilla Iván , López-Osorio Maria A. , Zurita-Gil Manuel A. , Calixto-Rodriguez Manuela , Rosales Antonio Márquez , Juárez Mario A.
Published: September 2024

Agriculture drones face important challenges regarding autonomy and construction, as flying time below the 9-minute mark is the norm, and their manufacture requires several tests and research before reaching proper flight dynamics. Therefore, correct design, analysis, and manufacture of the structure are imperative to address the aforementioned problems and ensure a robust build that withstands the tough environments of this application. In this work, the analysis and implementation of a Nylamid motor bracket, aluminum sandwich-type skeleton, and carbon fiber tube arm in a 30 kg agriculture drone is presented. The mechanical response of these components is evaluated using the finite element method in ANSYS Workbench, and the material behavior assumptions are assessed using a universal testing machine before their implementations. The general description of these models and the numerical results are presented. This early prediction of the behavior of the structure allows for mass optimization and cost reductions. The fast dynamics of drone applications set important restrictions in ductile materials such as this, requiring extensive structural analysis before manufacture. Experimental and numerical results showed a maximum variation of 8.7% for the carbon fiber composite and 13% for the Nylamid material. The mechanical properties of polyamide nylon allowed for a 51% mass reduction compared to a 6061 aluminum alloy structure optimized for the same load case in the motor brackets design. The low mechanical complexity of sandwich-type skeletons translated into fast implementation. Finally, the overall performance of the agriculture drone is evaluated through the data gathered during the flight test, showing the adequate design process.
Nutritional, Bioactive, and Volatile Characteristics of Two Types of Sorbus domestica Undervalued Fruit from Northeast of Iberian Peninsula, Spain
Authors: Raigón Jiménez María Dolores , García-Martínez María Dolores , Esteve Ciudad Patricia , Fukalova Fukalova Tamara
Published: September 2024

The promotion of food from underutilized plants can help combat biodiversity loss, foster cultural preservation, and empower farmers in the face of market pressures and sustainable production conditions. The nutritional and aromatic characterization of two undervalued types of Sorbus domestica fruits, differentiated by their apple and pear shapes, has been carried out. Official Association of Analytical Communities methods have been used for proximate composition and mineral analysis determinations, and gas chromatography was used for the analysis of volatile components in three states of ripeness and compared with the aromas of fresh apple and quince jam. S. domestica fruits are a good source of K, Ca, Fe, and fiber and are an important source of antioxidants in the human diet. S. domestica fruits have proven to be very distinctive in the aromatic fraction. 1-hexanol, hexyl 1,3-octanediol, phenylacetaldehyde, nonanal, hexanal, and α-farnesene are the most potent odor compounds in the overripening stage of the fruits. The aroma profiles of immature S. domestica fruits were dominated by aldehydes, while in the overripe stage, the fruit accumulated abundant esters, alcohols, and sesquiterpenoids. S. domestica fruits could be introduced as an alternative to seasonal fruit consumption and could generate sustainable production and consumption alternatives while recovering cultural and food heritage.
A Stereoselective Synthesis of a Novel α,β-Unsaturated Imine-Benzodiazepine through Condensation Reaction, Crystal Structure, and DFT Calculations
Authors: Hmaimou Samir , Ait Lahcen Marouane , Adardour Mohamed , Alanazi Mohammed M. , Kabra Atul , Maatallah Mohamed , Baouid Abdesselam
Published: September 2024

The stereoisomers (E)-2,2-dimethyl-4-(4-subsitutedstyryl)-2,3-dihydro-1H-[1,5]-benzodiazepine 3(a–d) were synthesized via the condensation reaction of 2,2,4-trimethyl-2,3-dihydro-1H-1,5-benzodiazepine (BZD) 1 with the benzaldehyde derivatives 2(a–d) in ethanol. The chemical structure of the prepared products was confirmed by NMR (1H and 13C), HRMS, and X-ray analysis of the crystal structure 3d. The condensation reaction was examined using DFT calculations at the theoretical level of B3LYP/6-31G(d) to elucidate the chemo-, regio-, and stereoselectivity and the reaction mechanism of the produced isomer. Furthermore, we identified each reagent’s reactive sites by the measurement of the reactivity indices. We also looked at how the electron-withdrawing groups (EWGs) of various aldehydes affected the reaction’s mechanism and the stability of products 3(a–d).
Upgrading of Pyrolysis Bio-Oil by Catalytic Hydrodeoxygenation, a Review Focused on Catalysts, Model Molecules, Deactivation, and Reaction Routes
Authors: Carrasco Díaz Alejandra , Abdelouahed Lokmane , Brodu Nicolas , Montes-Jiménez Vicente , Taouk Bechara
Published: September 2024

Biomass can be converted into energy/fuel by different techniques, such as pyrolysis, gasification, and others. In the case of pyrolysis, biomass can be converted into a crude bio-oil around 50–75% yield. However, the direct use of this crude bio-oil is impractical due to its high content of oxygenated compounds, which provide inferior properties compared to those of fossil-derived bio-oil, such as petroleum. Consequently, bio-oil needs to be upgraded by physical processes (filtration, emulsification, among others) and/or chemical processes (esterification, cracking, hydrodeoxygenation, among others). In contrast, hydrodeoxygenation (HDO) can effectively increase the calorific value and improve the acidity and viscosity of bio-oils through reaction pathways such as cracking, decarbonylation, decarboxylation, hydrocracking, hydrodeoxygenation, and hydrogenation, where catalysts play a crucial role. This article first focuses on the general aspects of biomass, subsequent bio-oil production, its properties, and the various methods of upgrading pyrolytic bio-oil to improve its calorific value, pH, viscosity, degree of deoxygenation (DOD), and other attributes. Secondly, particular emphasis is placed on the process of converting model molecules and bio-oil via HDO using catalysts based on nickel and nickel combined with other active elements. Through these phases, readers can gain a deeper understanding of the HDO process and the reaction mechanisms involved. Finally, the different equipment used to obtain an improved HDO product from bio-oil is discussed, providing valuable insights for the practical application of this reaction in pyrolysis bio-oil production.
Polyethyleneimine Modified Two-Dimensional GO/MXene Composite Membranes with Enhanced Mg2+/Li+ Separation Performance for Salt Lake Brine
Authors: Wang Jun , Wang Andong , Liu Jiayuan , Niu Qiang , Zhang Yijia , Liu Ping , Liu Chengwen , Wang Hongshan , Zeng Xiangdong , Zeng Guangyong
Published: September 2024

As global demand for renewable energy and electric vehicles increases, the need for lithium has surged significantly. Extracting lithium from salt lake brine has become a cutting-edge technology in lithium resource production. In this study, two-dimensional (2D) GO/MXene composite membranes were fabricated using pressure-assisted filtration with a polyethyleneimine (PEI) coating, resulting in positively charged PEI-GO/MXene membranes. These innovative membranes, taking advantage of the synergistic effects of interlayer channel sieving and the Donnan effect, demonstrated excellent performance in Mg2+/Li+ separation with a mass ratio of 20 (Mg2+ rejection = 85.3%, Li+ rejection = 16.7%, SLi,Mg = 5.7) in simulated saline lake brine. Testing on actual salt lake brine in Tibet, China, confirmed the composite membrane’s potential for effective Mg2+/Li+ separation. In the actual brine test with high concentration, Mg2+/Li+ after membrane separation is 2.2, which indicates that the membrane can significantly reduce the concentration of Mg2+ in the brine. Additionally, the PEI-GO/MXene composite membrane demonstrated strong anti-swelling properties and effective divalent ion rejection. This research presents an innovative approach to advance the development of 2D membranes for the selective removal of Mg2+ and Li+ from salt lake brine.
Molecular Simulation Analysis of Polyurethane Molecular Structure under External Electric Field
Authors: Pang Zhiyi , Huang Shangshi , Li Yi , Zhang Yiyi , Qin Rui
Published: September 2024

Polyurethane (PU) materials are extensively utilized in power equipment. This paper introduces a comprehensive evaluation method that combines electromagnetics and computational chemistry based on the Density Functional Theory (DFT) to elucidate the impact of external electric fields on the molecular structure of PU during electrical contact. The study focuses on the microstructural and molecular energy changes in the hard (HS) and soft (SS) segments of PU under the influence of an electric field of uniform intensity. Findings indicate that the total energy of HS molecules decreases markedly as the electric field intensity increases, accompanied by a significant rise in both the dipole moment and polarizability. Conversely, the total energy and polarizability of the SS molecules decrease, while the dipole moment experiences a slight increase. Under the influence of a strong electric field, HS molecules tend to stretch towards the extremities of the main chain, leading to structural instability and the cleavage of hydroxyl O-H bonds. Meanwhile, the carbon chain of the SS molecules twists towards the center under the electric field, with no chemical bond rupture observed. At an electric field intensity of 8.227 V/nm, the HOMO-LUMO gap of the HS molecule narrows sharply, signifying a rapid decline in the molecular structure stability, corroborated by infrared spectroscopy analysis. These findings offer theoretical insights and guidance for the modification of PU materials in power equipment applications.
Use of Molecular Logic Gates for the Tuning of Chemosensor Dynamic Range
Authors: Acikgoz Orhan , Abelt Christopher
Published: September 2024

Dynamic range is a crucial aspect in the development of fluorescent chemosensors. We aimed to address this issue using molecular logic gates. By creating an AND logic gate with two binding sites for the same type of ion, we increased the dynamic range of a sodium chemosensor while still using the same ionophore. Naphthalimide derivatives 1 and 2 were synthesized to test the plausibility of this application. Being an AND logic gate, the second molecule requires two Na+ ions, while molecule 1 requires a single ion for sensing. The application of this molecular logic gate is a useful method of altering the chemosensor range.
An Acid-Responsive Fluorescent Molecule for Erasable Anti-Counterfeiting
Authors: Liu Jiabao , Gao Xiangyu , Niu Qingyu , Jin Mingyuan , Wang Yijin , Alshahrani Thamraa , Sun He-Lue , Chen Banglin , Li Zhiqiang , Li Peng
Published: September 2024

A tetraphenylethylene (TPE) derivative, TPEPhDAT, modified by diaminotriazine (DAT), was prepared by successive Suzuki–Miyaura coupling and ring-closing reactions. This compound exhibits aggregation-induced emission enhancement (AIEE) properties in the DMSO/MeOH system, with a fluorescence emission intensity in the aggregated state that is 5-fold higher than that of its counterpart in a dilute solution. Moreover, the DAT structure of the molecule is a good acceptor of protons; thus, the TPEPhDAT molecule exhibits acid-responsive fluorescence. TPEPhDAT was protonated by trifluoroacetic acid (TFA), leading to fluorescence quenching, which was reversibly restored by treatment with ammonia (on–off switch). Time-dependent density functional theory (TDDFT) computational studies have shown that protonation enhances the electron-withdrawing capacity of the triazine nucleus and reduces the bandgap. The protonated TPEPhDAT conformation became more distorted, and the fluorescence lifetime was attenuated, which may have produced a twisted intramolecular charge transfer (TICT) effect, leading to fluorescence redshift and quenching. MeOH can easily remove the protonated TPEPhDAT, and this acid-induced discoloration and erasable property can be applied in anti-counterfeiting.
Synthesis and Structure of 5-Methyl-9-(trifluoromethyl)-12H-quino[3,4-b][1,4]benzothiazinium Chloride as Anticancer Agent
Authors: Zieba Andrzej , Kozik Violetta , Suwinska Kinga , Kawulok Agata , Pluta Tadeusz , Jampilek Josef , Bak Andrzej
Published: September 2024

In this work, the synthesis, structural analysis and anticancer properties of 5-methyl-9-trifluoromethyl-12H-quino[3,4-b][1,4]benzothiazinium chloride (3) are described. Compound 3 was synthesized by reacting 1-methyl-4-butylthio-3-(benzoylthio)quinolinium chloride with 4-(trifluoromethyl)aniline, respectively. The structure of the resulting product was determined using 1H-NMR and 13C-NMR spectroscopy as well as HR-MS spectrometry. The spatial geometry of agent 3 and the arrangement of molecules in the crystal (unit cell) were also confirmed using X-ray diffraction. The tetracyclic quinobenzothiazinium system is fairly planar because the dihedral angle between the planes formed by the benzene ring and the quinoline system is 173.47°. In order to obtain insight into the electronic charge distribution of the investigated molecule, electronic structure calculations employing the Density Functional Theory (DFT) were performed. Moreover, antiproliferative activity against a set of pancreatic cancer cell lines was tested, with compound 3 showing IC50 values against human primary pancreatic adenocarcinoma BxPC-3 and human epithelioid pancreatic carcinoma Panc-1 of 0.051 µM and 0.066 µM, respectively. The IC50 value of cytotoxicity/cell viability of the investigated compound assessed on normal human lung fibroblasts WI38 was 0.36 µM.
Neurotoxicity, Neuroprotection, In Vitro MAOA/MAOB Inhibitory Activity Assessment, Molecular Docking, and Permeability Assay Studies of Newly Synthesized Hydrazones Containing a Pyrrole Ring
Authors: Georgieva Maya , Mateev Emilio , Valkova Iva , Kuteva Hristina , Tzankova Diana , Stefanova Denitsa , Yordanov Yordan , Lybomirova Karolina , Zlatkov Alexander , Tzankova Virginia , Kondeva-Burdina Magdalena
Published: September 2024

Neurodegenerative diseases such as Parkinson’s and Alzheimer’s continue to be some of the most significant challenges in modern medicine. Recent research related to the molecular mechanisms of parkinsonism has opened up new approaches to antiparkinsonian therapy. In response to this, we present the evaluation of the potential neuroprotective and MAOA/MAOB inhibitory effects of newly synthesized hydrazones, containing a pyrrole moiety in the carboxyl fragment of the structure. The substances were studied on different brain subcellular fractions, including rat brain synaptosomes, mitochondria, and microsomes. The single application of 50 µM of each compound to the subcellular fractions showed that all substances exhibit a weak neurotoxic effect, with 7b, 7d, and 8d being the least neurotoxic representatives. The corresponding neuroprotective and antioxidant effects were also evaluated in different injury models on subcellular fractions, single out 7b, 7d, and 8d as the most prominent derivatives. A 1 µM concentration of each molecule from the series was also studied for potential hMAOA/hMAOB inhibitory effects. The results revealed a lack of hMAOA activity for all evaluated structures and the appearance of hMAOB effects, with compounds 7b, 7d, and 8d showing effects similar to those of selegiline. The best hMAOB selectivity index (>204) was determined for 7d and 8d, distinguishing these two representatives as the most promising molecules for further studies as potential selective MAOB inhibitors. The performed molecular docking simulations defined the appearance of selective MAOB inhibitory effects based on the interaction of the tested molecules with Tyr398, which is one of the components of the aromatic cage of MAOB and participated in π–π stabilization with the aromatic pyrrole ring. The preliminary PAMPA testing indicated that in relation to the blood–brain barrier (BBB) permeability, the tested pyrrole-based hydrazones may be considered as high permeable, except for 8a and 8e, which were established to be permeable in the medium range with −logP of 5.268 and 5.714, respectively, compared to the applied references.
Synthesis and Antimicrobial Activity of (E)-1-Aryl-2-(1H-tetrazol-5-yl)acrylonitrile Derivatives via [3+2] Cycloaddition Reaction Using Reusable Heterogeneous Nanocatalyst under Microwave Irradiation
Authors: Nanda Ayashkanta , Kaur Navneet , Kaur Manvinder , Husain Fohad Mabood , Han Haesook , Bhowmik Pradip K. , Sohal Harvinder Singh
Published: September 2024

The magnetically recoverable heterogeneous Fe2O3@cellulose@Mn nanocomposite was synthesized by a stepwise fabrication of Mn nanoparticles on cellulose-modified magnetic Fe2O3 nanocomposites, and the morphology of the nanocomposite was characterized through advanced spectroscopic techniques. This nanocomposite was investigated as a heterogeneous catalyst for the synthesis of medicinally important tetrazole derivatives through Knoevenagel condensation between aromatic/heteroaromatic aldehyde and malononitrile followed by [3+2] cycloaddition reaction with sodium azide. Thirteen potent (E)-1-aryl-2-(1H-tetrazol-5-yl)acrylonitrile derivatives are reported in this paper with very high yields (up to 98%) and with excellent purity (as crystals) in a very short period (3 min @ 120 W) using microwave irradiation. The present procedure offers several advantages over recent protocols, including minimal catalyst loading, quick reaction time, and the utilization of an eco-friendly solvent. Furthermore, the synthesized (E)-1-aryl-2-(1H-tetrazol-5-yl)acrylonitrile derivatives (4b, 4c, and 4m) are shown to have excellent resistance against various fungal strains over bacterial strains as compared to the standard drugs Cefixime (4 μg/mL) and Fluconazole (2 μg/mL).
Preliminary Evidence That Design Fluency Is Related to Dual-Task Treadmill Gait Variability in Healthy Adults
Authors: Higginson Christopher I. , Bifano Morgan K. , Seymour Kelly M. , Orr Rachel L. , DeGoede Kurt M. , Higginson Jill S.
Published: September 2024

Evidence supporting a link between gait and cognition is accumulating. However, the relation between executive functioning and spatiotemporal gait parameters has received little attention. This is surprising since these gait variables are related to falls. The goal of this preliminary study was to determine whether performance on measures of inhibition, reasoning, and fluency is related to variability in stride length and step width during dual-task treadmill walking in a sample of healthy adults. Nineteen healthy adults averaging 40 years of age were evaluated. Results indicated that processing speed was reduced, t(18) = 6.31, p = 0.0001, step width increased, t(18) = −8.00, p = 0.0001, and stride length decreased, t(18) = 3.06, p = 0.007, while dual tasking, but variability in gait parameters did not significantly change, consistent with a gait/posture-first approach. As hypothesized, better performance on a visual design fluency task which assesses cognitive flexibility was associated with less dual-task stride length variability, rs(17) = −0.43, p = 0.034, and step width variability, r = −0.56, p = 0.006. The results extend previous findings with older adults walking over ground and additionally suggest that cognitive flexibility may be important for gait maintenance while dual tasking.
Genomics Insights into Mycolicibacterium Hassiacum Causing Infection in a Cat with Pyogranulomatous Dermatitis and Panniculitis
Authors: Smedile Daniele , Iurescia Manuela , Carfora Virginia , Cocumelli Cristiano , Palmerini Tiziana , Diaconu Elena Lavinia , Congiu Ilaria , Donati Valentina , Stravino Fiorentino , Sorbara Luigi , Romano Erica , Caprioli Andrea , Battisti Antonio
Published: September 2024

Mycolicibacterium hassiacum (homotypic synonym: Mycobacterium hassiacum) represents an ungrouped thermotolerant rapidly growing mycobacteria (RGM) species occasionally associated with infections and disease in humans. In this report, we describe a case of pyogranulomatous dermatitis and panniculitis due to M. hassiacum in an immunocompetent adult cat. To the best of our knowledge, this represents the first report of M. hassiacum infection in animals. We also report the results of the in-depth genome characterization of the isolate using a combined short- and long-read whole-genome sequencing (WGS) approach. We observed the lack of acquired-resistance genes and no evidence of mutations in housekeeping genes associated with resistance to rifampicin and isoniazid. We detected some virulence factors in our isolate, such as some associated with the interaction of mycobacteria with host cells, and the presence of multiple copies of heavy metal resistance genes (arsB, arsR, and arsL/cadL). In conclusion, M. hassiacum should be included among the RGM species associated with feline subcutaneous atypical mycobacteriosis (SAM). A reliable and fast RGM laboratory identification and characterization is important not only for an accurate etiological diagnosis but also for a correct approach to SAM treatment options.
Gastrodin Alleviates Angiotensin II-Induced Hypertension and Myocardial Apoptosis via Inhibition of the PRDX2/p53 Pathway In Vivo and In Vitro
Authors: Xu Nanhui , Xie Qiurong , Chen Youqin , Li Jiapeng , Zhang Xiuli , Zheng Huifang , Cheng Ying , Wu Meizhu , Shen Aling , Wei Lihui , Yao Mengying , Yang Yanyan , Sferra Thomas J. , Jafri Anjum , Fang Yi , Peng Jun
Published: September 2024

Gastrodin, a highly potent compound found in the traditional Chinese medicine Gastrodia elata Blume, exhibits significant antihypertensive properties. However, its role and the mechanism behind its protective effects on hypertensive cardiac conditions are not well understood. This study aims to investigate the cardiac protective effects and underlying mechanisms of gastrodin in angiotensin II (Ang II)-induced hypertensive models, both in vivo and in vitro. Treatment with gastrodin significantly decreased blood pressure and the heart weight/tibial length (HW/TL) ratio and attenuated cardiac dysfunction and pathological damage in Ang II-infused C57BL/6 mice. RNA sequencing analysis (RNA-seq) revealed 697 up-regulated and 714 down-regulated transcripts, along with 1105 signaling pathways, in Ang II-infused C57BL/6 mice following gastrodin treatment, compared to Ang II-induced hypertensive mice. Furthermore, the analyses of the top 30 Gene Ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway indicated significant enrichment in apoptosis and the peroxiredoxin 2 (PRDX2)/p53 pathway. Consistently, gastrodin treatment significantly reduced myocardial apoptosis in both the cardiac tissues of Ang II-induced hypertensive mice and Ang II-stimulated H9c2 cells. Additionally, gastrodin treatment significantly decreased the protein levels of PRDX2, p53, cleaved caspase-3, cleaved caspase-9, and Bax/Bcl-2 ratio in the cardiac tissues of Ang II-infused mice and H9c2 cells stimulated with Ang II. In conclusion, gastrodin treatment can mitigate hypertension-induced myocardial apoptosis in hypertensive mice by inhibiting the PRDX2/p53 pathway.
CAVPENET Peptide Inhibits Prostate Cancer Cells Proliferation and Migration through PP1γ-Dependent Inhibition of AKT Signaling
Authors: Matos Bárbara , Gomes Antoniel A. S. , Bernardino Raquel , Alves Marco G. , Howl John , Jerónimo Carmen , Fardilha Margarida
Published: September 2024

Protein phosphatase 1 (PP1) complexes have emerged as promising targets for anticancer therapies. The ability of peptides to mimic PP1-docking motifs, and so modulate interactions with regulatory factors, has enabled the creation of highly selective modulators of PP1-dependent cellular processes that promote tumor growth. The major objective of this study was to develop a novel bioactive cell-penetrating peptide (bioportide), which, by mimicking the PP1-binding motif of caveolin-1 (CAV1), would regulate PP1 activity, to hinder prostate cancer (PCa) progression. The designed bioportide, herein designated CAVPENET, and a scrambled homologue, were synthesized using microwave-assisted solid-phase methodologies and evaluated using PCa cell lines. Our findings indicate that CAVPENET successfully entered PCa cells to influence both viability and migration. This tumor suppressor activity of CAVPENET was attributed to inhibition of AKT signaling, a consequence of increased PP1γ activity. This led to the suppression of glycolytic metabolism and alteration in lipid metabolism, collectively representing the primary mechanism responsible for the anticancer properties of CAVPENET. Our results underscore the potential of the designed peptide as a novel therapy for PCa patients, setting the stage for further testing in more advanced models to fully realize its therapeutic promise.
Ultra-Wideband Cross-Polarization Converter Using Metasurface Operating in the X- and K-Band
Authors: Abbas Muhammad Basir , Raza Faizan , Baqir Muhammad Abuzar , Altintas Olcay , Abbas Musarat , KaraaSlan Muharrem , Naqvi Qaisar Abbas
Published: September 2024

The ultra-wideband polarization converters have been of interest to researcher due to their demand in satellite communication and navigation systems. This paper presents an ultra-wideband reflective cross-polarization converter comprising a stair-shaped metasurface. By observation, the alleged structure allows the conversion of linearly polarized waves to orthogonal components, having a polarization conversion ratio of greater than 90% spread across the large frequency range of 12.94 to 16.54 GHz and 17.54 to 26 GHz. A highly efficient, ultra-high frequency polarization conversion is achieved by the utilization of strong electromagnetic resonance coupling between the upper and lower layers of the metasurface. Further, it is depicted that the polarization converter has a wide obliquity of incidence wave. Moreover, the simulation and measured results show a good match. The linear polarization converter is simple in design but is of high performance, and therefore, might be useful in satellite communication, imaging systems, and navigation systems.
Assessment of Population Genetic Diversity of Medicinal Meconopsis integrifolia (Maxim.) Franch. Using Newly Developed SSR Markers
Authors: Wu Jiahao , Yang Quanyin , Zhao Wanyue , Miao Xue , Qin Yuan , Qu Yan , Zheng Ping
Published: September 2024

Meconopsis integrifolia is an endangered Tibetan medicinal plant with significant medicinal and ornamental value. Understanding its genetic diversity and structure is crucial for its sustainable utilization and effective conservation. Here, we develop a set of SSR markers based on transcriptome data to analyze the genetic diversity and structure of 185 individuals from 16 populations of M. integrifolia. The results indicate that M. integrifolia exhibits relatively high genetic diversity at the species level (the percentage of polymorphic bands PPB = 91.67%, Nei’s genetic diversity index He = 0.2989, Shannon’s information index I = 0.4514) but limited genetic variation within populations (PPB = 12.08%, He = 0.0399, I = 0.0610). The genetic differentiation among populations is relatively high (the coefficient of gene differentiation GST = 0.6902), and AMOVA analysis indicates that 63.39% of the total variation occurs among populations. This suggests that maintaining a limited number of populations is insufficient to preserve the overall diversity of M. integrifolia. Different populations are categorized into four representative subclusters, but they do not cluster strictly according to geographical distribution. Limited gene flow (Nm = 0.2244) is likely the main reason for the high differentiation among these populations. Limited seed and pollen dispersal abilities, along with habitat fragmentation, may explain the restricted gene flow among populations, highlighting the necessity of conserving as many populations in the wild as possible.
Chemical-Functional Analysis of Extracts Obtained from Zuccagnia punctata Powder Using Green Solvents (NaDESs) in Conjunction with Traditional and Non-Traditional Techniques
Authors: Romero Antonela Mariana , Zampini Iris Catiana , Isla María Inés
Published: September 2024

Zuccagnia punctata Cav. (Family Fabaceae. Subfamily Caesalpinioideae) is a native plant species with a long history of use in Argentine traditional medicine. The purpose of the present study was to extract bioactive compounds with antioxidant and antifungal activity from Z. punctata aerial parts using conventional solvents (water, ethanol 60°, vegetal oil) and unconventional solvents (natural deep eutectic solvents or NaDESs) such as green solvents with and without the assistance of ultrasound (UAE) and microwaves (MAE). NaDESs such as glucose: lactic acid (LGH), sucrose: citric acid (CAS), choline chloride: urea (CU) and glucose: fructose: sucrose (FGS) were used. LGH and CU were effective in the extraction of phenolic compounds (6710 ± 10.12 µg GAE/mL and 7140 ± 15.00 µg GAE/mL, respectively) as well as ethanol (6270 µg ± 12.00 µg GAE/mL) using conventional methods. Two chemical markers of Z. punctata (2′,4′-dihydroxychalcone and 2′,4′-dihydroxy -3-methoxychalcone) were extracted in a high proportion in ethanol, oil, LGH and CU with UAE. The ABTS antioxidant capacity was higher in the extracts obtained with LGH and CU (SC50: 0.90 ± 0.10 µg GAE/mL and 1.08 ± 0.16 µg GAE/mL, respectively). The extract obtained with vegetal oil was the most potent as antifungal, followed by the extracts in ethanol, LGH and CU. These findings highlight the importance of using environmentally friendly solvents such as NaDESs to obtain bioactive metabolites from Z. punctata, an endemic plant of Argentina with a potential application in the food, cosmetic and pharmaceutical industries.
Prolonged Post-Harvest Preservation in Lettuce (Lactuca sativa L.) by Reducing Water Loss Rate and Chlorophyll Degradation Regulated through Lighting Direction-Induced Morphophysiological Improvements
Authors: Yang Jingli , Song Jinnan , Liu Jie , Dong Xinxiu , Zhang Haijun , Jeong Byoung Ryong
Published: September 2024

To investigate the relationship between the lighting direction-induced morphophysiological traits and post-harvest storage of lettuce, the effects of different lighting directions (top, T; top + side, TS; top + bottom, TB; side + bottom, SB; and top + side + bottom, TSB; the light from different directions for a sum of light intensity of 600 μmol·m−2·s−1 photosynthetic photon flux density (PPFD)) on the growth morphology, root development, leaf thickness, stomatal density, chlorophyll concentration, photosynthesis, and chlorophyll fluorescence, as well as the content of nutrition such as carbohydrates and soluble proteins in lettuce were analyzed. Subsequently, the changes in water loss rate, membrane permeability (measured as relative conductivity and malondialdehyde (MDA) content), brittleness (assessed by both brittleness index and β-galactosidase (β-GAL) activity), and yellowing degree (evaluated based on chlorophyll content, and activities of chlorophyllase (CLH) and pheophytinase (PPH)) were investigated during the storage after harvest. The findings indicate that the TS treatment can effectively reduce shoot height, increase crown width, enhance leaves’ length, width, number, and thickness, and improve chlorophyll fluorescence characteristics, photosynthetic capacity, and nutrient content in lettuce before harvest. Specifically, lettuce’s leaf thickness and stomatal density showed a significant increase. Reasonable regulation of water loss in post-harvested lettuce is essential for delaying chlorophyll degradation. It was utilized to mitigate the increase in conductivity and hinder the accumulation of MDA in lettuce. The softening speed of leafy vegetables was delayed by effectively regulating the activity of the β-GAL. Chlorophyll degradation was alleviated by affecting CLH and PPH activities. This provides a theoretical basis for investigating the relationship between creating a favorable light environment and enhancing the post-harvest preservation of leafy vegetables, thus prolonging their post-harvest storage period through optimization of their morphophysiological phenotypes.
Analysis and Simulation of Adsorption Efficiency of Herbicides Diuron and Linuron on Activated Carbon from Spent Coffee Beans
Authors: Moraes Luiz Eduardo Zani de , Marcoti Felipe Augusto Olivo , Lucio Marco Antônio Naves , Rocha Bianca Caroline da Silva , Rocha Lucas Bonfim , Romero Adriano Lopes , Bona Evandro , Peron Ana Paula , Junior Osvaldo Valarini
Published: September 2024

Phenyl urea herbicides such as diuron and linuron are commonly used in agriculture to eliminate weeds. Their uncontrolled use can cause environmental problems. In this study, the adsorption of these herbicides was evaluated using activated carbon from coffee grounds, activated with zinc chloride (AC-ZnCl2, 100% purity), nitric acid (AC-HNO3, 65% purity), and commercially activated (AC-C) carbon for comparison purposes. The spent coffee grounds were transformed into activated carbon through the calcination process. The highest removal efficiency for diuron 40 mg∙L−1 and linuron 31 mg∙L−1 was obtained using the ZnCl2-activated adsorbent, being 100% and 45%, respectively. The best pH range was between 4 and 6. Adsorption kinetic studies showed that pseudo-first and second-order models fit the experimental data, with the adsorption rate increasing rapidly within 60 min for the concentrations tested. Adsorption isotherms indicated that the Langmuir model provided the best fit for diuron, while the Freundlich model was more appropriate for linuron. The efficiency of the adsorption process using activated carbon (AC) was confirmed by the toxicity analysis of diuron and linuron solutions before and after adsorption with AC.
Advancements in Supercritical Carbon Dioxide Brayton Cycle for Marine Propulsion and Waste Heat Recovery
Authors: Alzuwayer Bashar , Alhashem Abdulwahab , Albannaq Mohammad , Alawadhi Khaled
Published: September 2024

The Supercritical Carbon Dioxide Brayton Cycle (sCO2-BC) is a highly efficient and eco-friendly alternative for marine propulsion. The adoption of sCO2-BC aligns with the industry’s focus on sustainability and can help meet emission regulations. In this context, the current study introduces a cascade system that harnesses the exhaust gases from a marine Gas Turbine Propulsion System to serve as a heat source for a bottoming Supercritical Carbon Dioxide Brayton Cycle (sCO2-BC), which facilitates an onboard heat recovery system. The investigation primarily focuses on the recompression cycle layouts of the sCO2-BC. To assess the performance of the bottoming cycle layouts and the overall cascade system, various parameters of the recompression sCO2-BC are analyzed. These parameters include the mass flow rate of CO2 in the bottoming cycle and the effectiveness of both the low-temperature recuperator (LTR) and the high-temperature recuperator (HTR). For conducting the cycle simulations, two codes are built and integrated; this first code models the thermodynamic cycle, while the second code models the recuperators. The research shows that incorporating the sCO2 Brayton Cycle as a bottoming cycle has the potential to greatly improve the efficiency of the entire system, increasing it from 54% to 59%. Therefore, it provides a useful framework for advancing energy-efficient gas turbine systems and future research.
How to Popularize Smartphones among Older Adults: A Narrative Review and a New Perspective with Self-Efficacy, Social Capital, and Individualized Instruction as Key Drivers
Authors: Kokubun Keisuke
Published: September 2024

Information and Communication Technology (ICT) such as smartphones has been attracting attention to prevent elderly people from becoming isolated. For this reason, recent research has proposed training methods for acquiring smartphone functions. However, since the types of smartphone functions required vary from person to person, a one-size-fits-all approach fails to engage all individuals adequately, leading to limited outcomes. On the other hand, with a view to social implementation, it is necessary to clarify a method that is effective in both cost and time. Previous research suggests that self-efficacy and social capital are the keys to acquiring smartphone skills among elderly people. Therefore, in this review, while looking back at previous research, we propose a study to demonstrate that by providing careful individual instruction by an experienced instructor to elderly people with little experience in smartphones and then having them take turns teaching other participants after the instruction, their self-efficacy and social capital can be increased, and a positive spiral effect can be achieved to maximize the improvement of smartphone skills widely.
Albert Camus’s Ethics of Revolution as a Model for Ethical Thinking on Violent Revolutions for Catholics
Authors: Onah Gideon Owogeka
Published: September 2024

In this paper, I propose an account of Camus’s ethics in which violent revolutions are never morally permissible but nonetheless acceptable or necessary. My main thesis in this paper is that Camus’s ethics of revolution and my defence of it, particularly the non-moral account of the permissibility of violent revolutions it comprises, can shed light on the reasonableness of participating in and supporting violent revolutions to some Catholics and the broader Christian community. My account of Camus’s ethics of revolution and argument for its tenability will be compelling to some Catholics because they affirm their intuition that violent revolutions are morally untenable and show why it is nevertheless reasonable.
Non-Orthogonal Serret–Frenet Parametrization Applied to Path Following of B-Spline Curves by a Mobile Manipulator
Authors: Dyba Filip , Frego Marco
Published: September 2024

A tool for path following for a mobile manipulator is herein presented. The control algorithm is obtained by projecting a local frame associated with the robot onto the desired path, thus obtaining a non-orthogonal moving frame. The Serret–Frenet frame moving along the curve is considered as a reference. A curve resulting from the control points of a B-spline in 2D or 3D is investigated as the desired path. It is used to show how the geometric continuity of the path has an impact on the performance of the robot in terms of undesired force spikes. This can be understood by looking at the curvature and, in 3D, at the torsion of the path. These unwanted effects vanish and better performance is achieved thanks to the change of the B-spline order. The theoretical results are confirmed by the simulation study for a mobile manipulator consisting of a non-holonomic wheeled base coupled with a holonomic robotic arm with three degrees of freedom (rotational and prismatic).
Quantifying the Training Loads and Corresponding Changes in Physical Qualities among Adolescent, Schoolboy Rugby League Players
Authors: Carron Michael A. , Scanlan Aaron T. , Doering Thomas M.
Published: September 2024

Objectives: The adolescent development period is critical for rugby league athletes, given the physical growth, neuromuscular adaptation, and skill acquisition that occurs. Secondary schools play an important role in the development of adolescent rugby league players; however, players may be selected into rugby league academies and development programs outside of school, as well as participating in additional sports. In turn, the training loads these young athletes accrue and the implications of these loads are currently unknown. Our aim was to quantify the training loads and concomitant changes in physical qualities of schoolboy and adolescent rugby league players during mesocycles within the pre-season and in-season phases. Design: This is a prospective experimental study. Methods: Twenty-one schoolboy rugby league players (16.2 ± 1.3 years) were monitored across separate 4-week mesocycles in the pre-season and in-season. Session frequency, duration, and the session rating of perceived exertion (sRPE) load were reported for all examples of training and match participation in the school rugby league program, as well as club and representative teams for any sport and personal strength and conditioning. Various physical qualities were assessed before and after each 4-week mesocycle. Results: The sRPE load that accumulated across the 4-week mesocycles was higher in the pre-season than the in-season (8260 ± 2021 arbitrary units [AU] vs. 6148 ± 980 AU, p < 0.001), with non-significant differences in accumulated session frequency and duration between phases. Session frequency, duration, and sRPE load differed (p < 0.05) between some weeks in an inconsistent manner during the pre-season and in-season mesocycles. Regarding physical qualities, improvements (p < 0.05) in the 10 m sprint test, Multistage Fitness Test, medicine ball throw, and 1-repetition maximum back squat and bench press performances were evident across the pre-season mesocycle, with declines (p < 0.05) in the 505-Agility Test, L-run Test, and 1-repetition maximum back squat performances across the in-season mesocycle. Conclusions: These novel training load data show schoolboy rugby league players experience considerable demands that may be suitable in developing several physical qualities during the pre-season but detrimental to maintaining such qualities across the in-season.
Power Bounds for the Numerical Radius of the Off-Diagonal 2 × 2 Operator Matrix
Authors: Altwaijry Najla , Dragomir Silvestru Sever , Feki Kais
Published: September 2024

In this paper, we employ a generalization of the Boas–Bellman inequality for inner products, as developed by Mitrinović–Pečarić–Fink, to derive several upper bounds for the 2p-th power with p≥1 of the numerical radius of the off-diagonal operator matrix 0AB*0 for any bounded linear operators A and B on a complex Hilbert space H. While the general matrix is not symmetric, a special case arises when B=A*, where the matrix becomes symmetric. This symmetry plays a crucial role in the derivation of our bounds, illustrating the importance of symmetric structures in operator theory.