bluuebunny/crossref_metadata_2025 huggingface dataset:
Suppression of Osteoclastogenesis by Melatonin: A Melatonin Receptor-Independent Action
Authors: Kim Hyung , Kim Ha , Bae Moon-Kyoung , Kim Yong-Deok
Published: May 2017

In vertebrates, melatonin is primarily secreted from the pineal gland but it affects various biological processes including the sleep-wake cycle, vasomotor control, immune system and bone homeostasis. Melatonin has been known to promote osteoblast differentiation and bone maturation, but a direct role of melatonin on osteoclast differentiation is still elusive. The present study investigated the effect of melatonin on the differentiation of macrophages to osteoclasts. The presence of melatonin significantly reduced receptor activator of nuclear factor κB ligand (RANKL)-induced osteoclastogenesis and the siRNA-mediated knockdown of the melatonin receptor failed to overcome the anti-osteoclastogenic effect of melatonin. Although melatonin treatment did not affect the phosphorylation of extracellular signal-regulated kinase (ERK), p38 and c-Jun N-terminal kinase (JNK), it markedly inhibited the activation of NF-κB and subsequent induction of nuclear factor of activated T cell cytoplasmic 1(NFATc1). Thus, our results suggest that melatonin could suppress osteoclast differentiation through downregulation of NF-κB pathway with concomitant decrease in the NFATc1 transcription factor induction. Furthermore, melatonin seems to have an anti-osteoclastogenic effect independent of plasma membrane melatonin receptors. In addition to previously reported properties of melatonin, our study proposes another aspect of melatonin and bone homeostasis.
Nerve Growth Factor and Related Substances: A Brief History and an Introduction to the International NGF Meeting Series
Authors: Bradshaw Ralph A. , Mobley William , Rush Robert A.
Published: May 2017

Nerve growth factor (NGF) is a protein whose importance to research and its elucidation of fundamental mechanisms in cell and neurobiology far outstrips its basic physiological roles. It was the first of a broad class of cell regulators, largely acting through autocrine and paracrine interactions which will be described herein. It was of similar significance in establishing the identity and unique roles of neurotrophic factors in the development and maintenance of the peripheral and central nervous systems. Finally, it contributed to many advances in the elaboration of cell surface receptor mechanisms and intracellular cell signaling. As such, it can be considered to be a “molecular Rosetta Stone”. In this brief review, the highlights of these various studies are summarized, particularly as illustrated by their coverage in the 13 NGF international meetings that have been held since 1986.
Strawberry (cv. Romina) Methanolic Extract and Anthocyanin-Enriched Fraction Improve Lipid Profile and Antioxidant Status in HepG2 Cells
Authors: Forbes-Hernández Tamara , Gasparrini Massimiliano , Afrin Sadia , Cianciosi Danila , González-Paramás Ana , Santos-Buelga Celestino , Mezzetti Bruno , Quiles José , Battino Maurizio , Giampieri Francesca , Bompadre Stefano
Published: May 2017

Dyslipidemia and oxidation of low density lipoproteins (LDL) are recognized as critical factors in the development of atherosclerosis. Healthy dietary patterns, with abundant fruit and vegetable consumption, may prevent the onset of these risk factors due to the presence of phytochemical compounds. Strawberries are known for their high content of polyphenols; among them, flavonoids are the major constituents, and it is presumed that they are responsible for the biological activity of the fruit. Nevertheless, there are only a few studies that actually evaluate the effects of different fractions isolated from strawberries. In order to assess the effects of two different strawberry extracts (whole methanolic extract/anthocyanin-enriched fraction) on the lipid profile and antioxidant status in human hepatocellular carcinoma (HepG2) cells, the triglycerides and LDL-cholesterol content, lipid peroxidation, intracellular reactive oxygen species (ROS) content and antioxidant enzymes’ activity on cell lysates were determined. Results demonstrated that both strawberry extracts not only improved the lipid metabolism by decreasing triglycerides and LDL-cholesterol contents, but also improved the redox state of HepG2 cells by modulating thiobarbituric acid-reactive substances production, antioxidant enzyme activity and ROS generation. The observed effects were more pronounced for the anthocyanin-enriched fraction.
The Effect of Dry Eye Disease on Scar Formation in Rabbit Glaucoma Filtration Surgery
Authors: Ji Hong , Zhu Yingting , Zhang Yingying , Jia Yu , Li Yiqing , Ge Jian , Zhuo Yehong
Published: May 2017

The success rate of glaucoma filtration surgery is closely related to conjunctival inflammation, and the main mechanism of dry eye disease (DED) is inflammation. The aim of this study was to evaluate the effect of DED on bleb scar formation after rabbit glaucoma filtration surgery. Sixteen New Zealand white rabbits were randomly divided into control and DED groups. A DED model was induced by twice-daily topical administration of 0.1% benzalkonium chloride (BAC) drops for three weeks. Ocular examinations were performed to verify the DED model. Surgical effects were assessed, and histologic assessments were performed on the 28th postoperative day. Higher fluorescein staining scores, lower basal tear secretion levels and goblet cell counts, and increased interleukin 1β (IL-1β) levels were observed in the DED group. The DED eyes displayed significantly higher intraocular pressure (IOP)% on the 14th postoperative day; a smaller bleb area on days 14, 21 and 28; and a shorter bleb survival time. Moreover, proliferating cell nuclear antigen (PCNA) and alpha-smooth muscle actin (α-SMA) levels were significantly increased in the DED group. These results demonstrate that DED promotes filtering bleb scar formation and shortens bleb survival time; these effects may be mediated via IL-1β.
A Transcriptome Survey Spanning Life Stages and Sexes of the Harlequin Bug, Murgantia histrionica
Authors: Sparks Michael , Rhoades Joshua , Nelson David , Kuhar Daniel , Lancaster Jason , Lehner Bryan , Tholl Dorothea , Weber Donald , Gundersen-Rindal Dawn
Published: May 2017

The harlequin bug, Murgantia histrionica (Hahn), is an agricultural pest in the continental United States, particularly in southern states. Reliable gene sequence data are especially useful to the development of species-specific, environmentally friendly molecular biopesticides and effective biolures for this insect. Here, mRNAs were sampled from whole insects at the 2nd and 4th nymphal instars, as well as sexed adults, and sequenced using Illumina RNA-Seq technology. A global assembly of these data identified 72,540 putative unique transcripts bearing high levels of similarity to transcripts identified in other taxa, with over 99% of conserved single-copy orthologs among insects being detected. Gene ontology and protein family analyses were conducted to explore the functional potential of the harlequin bug’s gene repertoire, and phylogenetic analyses were conducted on gene families germane to xenobiotic detoxification, including glutathione S-transferases, carboxylesterases and cytochrome P450s. Genic content in harlequin bug was compared with that of the closely related invasive pest, the brown marmorated stink bug, Halyomorpha halys (Stål). Quantitative analyses of harlequin bug gene expression levels, experimentally validated using quantitative real-time PCR, identified genes differentially expressed between life stages and/or sexes.
Innate and Adaptive Immune Defects in Chronic Pulmonary Aspergillosis
Authors: Bongomin Felix , Harris Chris , Foden Philip , Kosmidis Chris , Denning David W.
Published: May 2017

We evaluated the expression of biomarkers of innate and adaptive immune response in correlation with underlying conditions in 144 patients with chronic pulmonary aspergillosis (CPA). Patients with complete medical and radiological records, white cell counts, and a complete panel of CD3, CD4, CD8, CD19, and CD56 lymphocyte subsets were included. Eighty-four (58%) patients had lymphopenia. Six (4%) patients had lymphopenia in all five CD variables. There were 62 (43%) patients with low CD56 and 62 (43%) patients with low CD19. Ten (7%) patients had isolated CD19 lymphopenia, 18 (13%) had isolated CD56 lymphopenia, and 15 (10%) had combined CD19 and CD56 lymphopenia only. Forty-eight (33%) patients had low CD3 and 46 (32%) had low CD8 counts. Twenty-five (17%) patients had low CD4, 15 (10%) of whom had absolute CD4 counts <200/μL. Multivariable logistic regression showed associations between: low CD19 and pulmonary sarcoidosis (Odds Ratio (OR), 5.53; 95% Confidence Interval (CI), 1.43–21.33; p = 0.013), and emphysema (OR, 4.58; 95% CI; 1.36–15.38; p = 0.014), low CD56 and no bronchiectasis (OR, 0.27; 95% CI, 0.10–0.77; p = 0.014), low CD3 and both multicavitary CPA disease (OR, 2.95; 95% CI, 1.30–6.72; p = 0.010) and pulmonary sarcoidosis (OR, 4.94; 95% CI, 1.39–17.57; p = 0.014). Several subtle immune defects are found in CPA.
Quantifying the National Significance of Local Areas for Regional Conservation Planning: North Carolina’s Mountain Treasures
Authors: Belote R. , Irwin G.
Published: May 2017

Conservation scientists recognize that additional protected areas are needed to maintain biological diversity and ecological processes. As regional conservation planners embark on recommending additional areas for protection in formal ecological reserves, it is important to evaluate candidate lands for their role in building a resilient protected areas system of the future. Here, we evaluate North Carolina’s Mountain Treasures with respect to their (1) ecological integrity, (2) role in connecting existing core protected areas, (3) potential to diversify the ecosystem representation of reserves, and (4) role in maintaining hotspots of biologically-rich areas that are not well protected. Mountain Treasures represent a citizen inventory of roadless areas and serve as candidates for elevated levels of conservation protection on U.S. federal lands. We compared Mountain Treasures to other candidate lands throughout the country to evaluate their potential national significance. While the Mountain Treasures tended to be more impacted by human modifications than other roadless areas, they are as important as other roadless areas with respect to their role in connecting existing protected areas and diversifying representation of ecosystems in conservation reserves. However, Mountain Treasures tended to have a much higher biodiversity priority index than other roadless areas leading to an overall higher composite score compared to other roadless areas. Our analysis serves as an example of how using broad-scale datasets can help conservation planners assess the national significance of local areas.
Magnetic and Structural Properties of Barium Hexaferrite BaFe12O19 from Various Growth Techniques
Authors: Vinnik Denis , Tarasova Aleksandra , Zherebtsov Dmitry , Gudkova Svetlana , Galimov Damir , Zhivulin Vladimir , Trofimov Evgeny , Nemrava Sandra , Perov Nikolai , Isaenko Ludmila , Niewa Rainer
Published: May 2017

Barium hexaferrite powder samples with grains in the μm-range were obtained from solid-state sintering, and crystals with sizes up to 5 mm grown from PbO, Na2CO3, and BaB2O4 fluxes, respectively. Carbonate and borate fluxes provide the largest and structurally best crystals at significantly lower growth temperatures of 1533 K compared to flux-free synthesis (1623 K). The maximum synthesis temperature can be further reduced by the application of PbO-containing fluxes (down to 1223 K upon use of 80 at % PbO), however, Pb-substituted crystals Ba1–xPbxFe12O19 with Pb contents in the range of 0.23(2) ≤ x ≤ 0.80(2) form, depending on growth temperature and flux PbO content. The degree of Pb-substitution has only a minor influence on unit cell and magnetic parameters, although the values for Curie temperature, saturation magnetization, as well as the coercivity of these samples are significantly reduced in comparison with those from samples obtained from the other fluxes. Due to the lowest level of impurities, the samples from carbonate flux show superior quality compared to materials obtained using other methods.
A Constitutive Model for Soft Clays Incorporating Elastic and Plastic Cross-Anisotropy
Authors: Castro Jorge , Sivasithamparam Nallathamby
Published: May 2017

Natural clays exhibit a significant degree of anisotropy in their fabric, which initially is derived from the shape of the clay platelets, deposition process and one-dimensional consolidation. Various authors have proposed anisotropic elastoplastic models involving an inclined yield surface to reproduce anisotropic behavior of plastic nature. This paper presents a novel constitutive model for soft structured clays that includes anisotropic behavior both of elastic and plastic nature. The new model incorporates stress-dependent cross-anisotropic elastic behavior within the yield surface using three independent elastic parameters because natural clays exhibit cross-anisotropic (or transversely isotropic) behavior after deposition and consolidation. Thus, the model only incorporates an additional variable with a clear physical meaning, namely the ratio between horizontal and vertical stiffnesses, which can be analytically obtained from conventional laboratory tests. The model does not consider evolution of elastic anisotropy, but laboratory results show that large strains are necessary to cause noticeable changes in elastic anisotropic behavior. The model is able to capture initial non-vertical effective stress paths for undrained triaxial tests and to predict deviatoric strains during isotropic loading or unloading.
Ultrathin Six-Band Polarization-Insensitive Perfect Metamaterial Absorber Based on a Cross-Cave Patch Resonator for Terahertz Waves
Authors: Cheng Yong , Huang Mu , Chen Hao , Guo Zhen , Mao Xue , Gong Rong
Published: May 2017

A simple design of an ultrathin six-band polarization-insensitive terahertz perfect metamaterial absorber (PMMA), composed of a metal cross-cave patch resonator (CCPR) placed over a ground plane, was proposed and investigated numerically. The numerical simulation results demonstrate that the average absorption peaks are up to 95% at six resonance frequencies. Owing to the ultra-narrow band resonance absorption of the structure, the designed PMMA also exhibits a higher Q factor (>65). In addition, the absorption properties can be kept stable for both normal incident transverse magnetic (TM) and transverse electric (TE) waves. The physical mechanism behind the observed high-level absorption is illustrated by the electric and power loss density distributions. The perfect absorption originates mainly from the higher-order multipolar plasmon resonance of the structure, which differs sharply from most previous studies of PMMAs. Furthermore, the resonance absorption properties of the PMMA can be modified and adjusted easily by varying the geometric parameters of the unit cell.
Neutron Diffraction Evaluation of Near Surface Residual Stresses at Welds in 1300 MPa Yield Strength Steel
Authors: Harati Ebrahim , Karlsson Leif , Svensson Lars-Erik , Pirling Thilo , Dalaei Kamellia
Published: May 2017

Evaluation of residual stress in the weld toe region is of critical importance. In this paper, the residual stress distribution both near the surface and in depth around the weld toe was investigated using neutron diffraction, complemented with X-ray diffraction. Measurements were done on a 1300 MPa yield strength steel welded using a Low Transformation Temperature (LTT) consumable. Near surface residual stresses, as close as 39 µm below the surface, were measured using neutron diffraction and evaluated by applying a near surface data correction technique. Very steep surface stress gradients within 0.5 mm of the surface were found both at the weld toe and 2 mm into the heat affected zone (HAZ). Neutron results showed that the LTT consumable was capable of inducing near surface compressive residual stresses in all directions at the weld toe. It is concluded that there are very steep stress gradients both transverse to the weld toe line and in the depth direction, at the weld toe in LTT welds. Residual stress in the base material a few millimeters from the weld toe can be very different from the stress at the weld toe. Care must, therefore, be exercised when relating the residual stress to fatigue strength in LTT welds.
Micro-Mechanical Response of an Al-Mg Hybrid System Synthesized by High-Pressure Torsion
Authors: Kawasaki Megumi , Jang Jae-il
Published: May 2017

This paper summarizes recent efforts to evaluate the potential for the formation of a metal matrix nanocomposite (MMNC) by processing two commercial bulk metals of aluminum and magnesium alloy through high-pressure torsion (HPT) at room temperature. After significant evolutions in microstructures, successful fabrication of an Al-Mg hybrid system was demonstrated by observing unique microstructures consisting of a multi-layered structure and MMNC. Moreover, the evolution of small-scale mechanical properties was examined through the novel technique of nanoindentation and the improvement in plasticity was estimated by calculating the strain rate sensitivity of the Al-Mg hybrid system after HPT. The present paper demonstrates that, in addition to conventional tensile testing, the nanoindentation technique is exceptionally promising for ultrafine-grained materials processed by HPT, where the samples may have small overall dimensions and include heterogeneity in the microstructure.
Coincidence Points of a Sequence of Multivalued Mappings in Metric Space with a Graph
Authors: Khan Muhammad , Azam Akbar , Mehmood Nayyar
Published: May 2017

In this article the coincidence points of a self map and a sequence of multivalued maps are found in the settings of complete metric space endowed with a graph. A novel result of Asrifa and Vetrivel is generalized and as an application we obtain an existence theorem for a special type of fractional integral equation. Moreover, we establish a result on the convergence of successive approximation of a system of Bernstein operators on a Banach space.
Characterization of Acetyl-CoA Carboxylases in the Basal Dinoflagellate Amphidinium carterae
Authors: Haq Saddef , Bachvaroff Tsvetan , Place Allen
Published: May 2017

Dinoflagellates make up a diverse array of fatty acids and polyketides. A necessary precursor for their synthesis is malonyl-CoA formed by carboxylating acetyl CoA using the enzyme acetyl-CoA carboxylase (ACC). To date, information on dinoflagellate ACC is limited. Through transcriptome analysis in Amphidinium carterae, we found three full-length homomeric type ACC sequences; no heteromeric type ACC sequences were found. We assigned the putative cellular location for these ACCs based on transit peptide predictions. Using streptavidin Western blotting along with mass spectrometry proteomics, we validated the presence of ACC proteins. Additional bands showing other biotinylated proteins were also observed. Transcript abundance for these ACCs follow the global pattern of expression for dinoflagellate mRNA messages over a diel cycle. This is one of the few descriptions at the transcriptomic and protein level of ACCs in dinoflagellates. This work provides insight into the enzymes which make the CoA precursors needed for fatty acid and toxin synthesis in dinoflagellates.
Induction of p53-Independent Apoptosis and G1 Cell Cycle Arrest by Fucoidan in HCT116 Human Colorectal Carcinoma Cells
Authors: Park Hye , Park Shin-Hyung , Jeong Jin-Woo , Yoon Dahye , Han Min , Lee Dae-Sung , Choi Grace , Yim Mi-Jin , Lee Jeong , Kim Do-Hyung , Kim Gi-Young , Choi Il-Whan , Kim Suhkmann , Kim Heui-Soo , Cha Hee-Jae , Choi Yung
Published: May 2017

It is well known that fucoidan, a natural sulfated polysaccharide present in various brown algae, mediates anticancer effects through the induction of cell cycle arrest and apoptosis. Nevertheless, the role of tumor suppressor p53 in the mechanism action of fucoidan remains unclear. Here, we investigated the anticancer effect of fucoidan on two p53 isogenic HCT116 (p53+/+ and p53−/−) cell lines. Our results showed that inhibition of cell viability, induction of apoptosis and DNA damage by treatment with fucoidan were similar in two cell lines. Flow cytometric analysis revealed that fucoidan resulted in G1 arrest in the cell cycle progression, which correlated with the inhibition of phosphorylation of retinoblastoma protein (pRB) and concomitant association of pRB with the transcription factor E2Fs. Furthermore, treatment with fucoidan obviously upregulated the expression of cyclin-dependent kinase (CDK) inhibitors, such as p21WAF1/CIP1 and p27KIP1, which was paralleled by an enhanced binding with CDK2 and CDK4. These events also commonly occurred in both cell lines, suggesting that fucoidan triggered G1 arrest and apoptosis in HCT116 cells by a p53-independent mechanism. Thus, given that most tumors exhibit functional p53 inactivation, fucoidan could be a possible therapeutic option for cancer treatment regardless of the p53 status.
Experimental Investigation of Thermal Fatigue Die Casting Dies by Using Response Surface Modelling
Authors: Abdulhadi Hassan , Ahmad Syarifah , Ismail Izwan , Ishak Mahadzir , Mohammed Ghusoon
Published: May 2017

Mechanical and thermal sequences impact largely on thermo-mechanical fatigue of dies in a die casting operations. Innovative techniques to optimize the thermo-mechanical conditions of samples are major focus of researchers. This study investigates the typical thermal fatigue in die steel. Die surface initiation and crack propagation were stimulated by thermal and hardness gradients, acting on the contact surface layer. A design of experiments (DOE) was developed to analyze the effect of as-machined surface roughness and die casting parameters on thermal fatigue properties. The experimental data were assessed on a thermo-mechanical fatigue life assessment model, being assisted by response surface methodology (RSM). The eminent valuation was grounded on the crack length, hardness properties and surface roughness due to thermal fatigue. The results were analyzed using analysis of variance method. Parameter optimization was conducted using response surface methodology (RSM). Based on the model, the optimal results of 26.5 μm crack length, 3.114 μm surface roughness, and 306 HV0.5 hardness properties were produced.
Precipitation Behavior of ωo Phase in Ti-37.5Al-12.5Nb Alloy
Authors: Ye Teng , Song Lin , Quan Maohua , He Jianping , Lin Junpin
Published: May 2017

Mutual transformation between α2 and ωo phases has been an interesting topic in recent years. In this study, martensitic α2 was obtained by air-cooling from 1250 °C in Ti-37.5Al-12.5Nb (at%) alloy while four ωo variants formed in the βo phase matrix during the cooling process. Nonetheless, only one ωo variant was observed at the periphery of the α2 plates in the βo phase and the orientation relationship between these two phases was [0001] α2//[\({1\overline{2}10}\)] ωo; (\({11\overline{2}0}\)) α2//(0002) ωo. Thin γ plates precipitated within the α2 phase and were thought to be related to the appearance of ωo phase. The redistribution of the compositions during the phase transformations was studied by energy dispersive X-ray spectroscopy analysis. The corresponding mechanisms of the phase transformations mentioned above are discussed.
Separation and Recovery of Iron and Rare Earth from Bayan Obo Tailings by Magnetizing Roasting and (NH4)2SO4 Activation Roasting
Authors: Zhou Yan , Yang He , Xue Xiang-xin , Yuan Shuai
Published: May 2017

A novel approach for recovery of iron and rare earth elements (REEs) from Bayan Obo tailings of Baotou, China, was developed by combining magnetizing roasting, magnetic separation, (NH4)2SO4 activation roasting, and water leaching. Thermodynamic analysis of carbothermal reduction was conducted to determine the temperature of magnetizing roasting, and it agreed well with the experimental results. The maximum recovery of Fe reached 77.8% at 600 °C, and the grade of total Fe in the magnetic concentrate was 56.3 wt. %. An innovative approach, using water to leach REEs after (NH4)2SO4 activation roasting, was used to extract REEs from magnetic separation tailings. The main influence factors of the leaching recovery during (NH4)2SO4 activation roasting, were investigated with the mass ratio of (NH4)2SO4 to magnetic separation tailings, roasting temperature and roasting time. The leaching recoveries of La, Ce and Nd reached 83.12%, 76.64% and 77.35%, respectively, under the optimized conditions: a mass ratio of 6:1, a roasting temperature of 400 °C and a roasting time of 80 min. Furthermore, the phase composition and reaction process during the (NH4)2SO4 activation roasting were analyzed with X-ray diffraction (XRD), energy dispersive X-ray spectroscopy & scanning electron microscopy (EDS-SEM) and thermogravimetry & differential scanning calorimetry (TG-DSC), and the leaching solution and leaching residue were also characterized.
A Study on Cutting Force of Machining In Situ TiB2 Particle-Reinforced 7050Al Alloy Matrix Composites
Authors: Xiong Yifeng , Wang Wenhu , Jiang Ruisong , Lin Kunyang
Published: May 2017

In situ TiB2 particle-reinforced 7050Al alloy matrix composites are a new category of particulate metal matrix composites with improved mechanical and physical properties. At present, the study of machining in situ TiB2/Al composite is limited and no specific study has been presented on cutting force. Based on previous work, experimental investigation of cutting in situ TiB2/Al composite was carried out in this study to investigate the cutting force, shear angle, mean friction angle, and shear stress. The results indicated that the feed rate, instead of cutting speed, has a significant influence on the shear angle, mean friction angle, shear stress, and forces, which is different from cutting ex situ SiC/Al composites. Meanwhile, based on Merchant’s theory, a force model, which consists of chip formation and ploughing force, was established to have a better understanding of force generation. A comparison of the results show an acceptable agreement between the force model and experiments. Additionally, at varying feed rates, the linear relationship between the shear angle and mean friction angle is still suitable for cutting in situ TiB2/7050Al alloy composites.
Modeling Hybridization Kinetics of Gene Probes in a DNA Biochip Using FEMLAB
Authors: Munir Ahsan , Waseem Hassan , Williams Maggie , Stedtfeld Robert , Gulari Erdogan , Tiedje James , Hashsham Syed
Published: May 2017

Microfluidic DNA biochips capable of detecting specific DNA sequences are useful in medical diagnostics, drug discovery, food safety monitoring and agriculture. They are used as miniaturized platforms for analysis of nucleic acids-based biomarkers. Binding kinetics between immobilized single stranded DNA on the surface and its complementary strand present in the sample are of interest. To achieve optimal sensitivity with minimum sample size and rapid hybridization, ability to predict the kinetics of hybridization based on the thermodynamic characteristics of the probe is crucial. In this study, a computer aided numerical model for the design and optimization of a flow-through biochip was developed using a finite element technique packaged software tool (FEMLAB; package included in COMSOL Multiphysics) to simulate the transport of DNA through a microfluidic chamber to the reaction surface. The model accounts for fluid flow, convection and diffusion in the channel and on the reaction surface. Concentration, association rate constant, dissociation rate constant, recirculation flow rate, and temperature were key parameters affecting the rate of hybridization. The model predicted the kinetic profile and signal intensities of eighteen 20-mer probes targeting vancomycin resistance genes (VRGs). Predicted signal intensities and hybridization kinetics strongly correlated with experimental data in the biochip (R2 = 0.8131).
Toxin Variability Estimations of 68 Alexandrium ostenfeldii (Dinophyceae) Strains from The Netherlands Reveal a Novel Abundant Gymnodimine
Authors: Martens Helge , Tillmann Urban , Harju Kirsi , Dell’Aversano Carmela , Tartaglione Luciana , Krock Bernd
Published: May 2017

Alexandrium ostenfeldii is a toxic dinoflagellate that has recently bloomed in Ouwerkerkse Kreek, The Netherlands, and which is able to cause a serious threat to shellfish consumers and aquacultures. We used a large set of 68 strains to the aim of fully characterizing the toxin profiles of the Dutch A. ostenfeldii in consideration of recent reports of novel toxins. Alexandrium ostenfeldii is known as a causative species of paralytic shellfish poisoning, and consistently in the Dutch population we determined the presence of several paralytic shellfish toxins (PST) including saxitoxin (STX), GTX2/3 (gonyautoxins), B1 and C1/C2. We also examined the production of spiroimine toxins by the Dutch A. ostenfeldii strains. An extensive liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis revealed a high intraspecific variability of spirolides (SPX) and gymnodimines (GYM). Spirolides included 13-desMethyl-spirolide C generally as the major compound and several other mostly unknown SPX-like compounds that were detected and characterized. Besides spirolides, the presence of gymnodimine A and 12-Methyl-gymnodimine A was confirmed, together with two new gymnodimines. One of these was tentatively identified as an analogue of gymnodimine D and was the most abundant gymnodimine (calculated cell quota up to 274 pg cell−1, expressed as GYM A equivalents). Our multi-clonal approach adds new analogues to the increasing number of compounds in these toxin classes and revealed a high strain variability in cell quota and in toxin profile of toxic compounds within a single population.
Silver Nanoparticles in Orthopedic Applications: New Insights on Their Effects on Osteogenic Cells
Authors: Castiglioni Sara , Cazzaniga Alessandra , Locatelli Laura , Maier Jeanette
Published: May 2017

Infections of orthopedic implants are associated with high morbidity. The emergence of antibiotic resistant strains and the tendency of microbes to form biofilms on orthopedic devices prompt the individuation of novel antimicrobial agents. Silver nanoparticles represent an interesting alternative, but their effects on bone cells need to be clarified. We focused on osteoblast-like cells and on bone marrow-mesenchymal stem cells and found that these cells are rather resistant to the cytotoxic effects of silver nanoparticles, with a half maximal inhibitory concentration around 25 µg/mL as detected by MTT assay. Within a month of treatment, osteoblast-like cells adapt to the presence of the nanoparticles by upregulating hsp70 as shown by western blot. Hsp70 overexpression correlates with the restoration of normal cell proliferation. No alterations in the extent and time requirements were detected in mesenchymal stem cell induced to differentiate in osteoblasts in the presence of silver nanoparticles. Because the concentrations of silver nanoparticles which show antimicrobial activity are lower than those exerting toxic effects on bone-forming cells in vitro, we suggest that silver nanoparticles might represent a challenging tool to fight infections in orthopedic implants.
Syntheses of Radioiodinated Pyrimidine-2,4,6-Triones as Potential Agents for Non-Invasive Imaging of Matrix Metalloproteinases
Authors: Breyholz Hans-Jörg , Kopka Klaus , Schäfers Michael , Wagner Stefan
Published: May 2017

Dysregulated expression or activation of matrix metalloproteinases (MMPs) is observed in many kinds of live-threatening diseases. Therefore, MMP imaging for example with radiolabelled MMP inhibitors (MMPIs) potentially represents a valuable tool for clinical diagnostics using non-invasive single photon emission computed tomography (SPECT) or positron emission tomography (PET) imaging. This work includes the organic chemical syntheses and in vitro evaluation of five iodinated barbiturate based MMPIs and the selection of derivative 9 for radiosyntheses of isotopologues [123I]9 potentially useful for MMP SPECT imaging and [124I]9 for MMP PET imaging.
FLOWERING LOCUS T Triggers Early and Fertile Flowering in Glasshouse Cassava (Manihot esculenta Crantz)
Authors: Bull Simon , Alder Adrian , Barsan Cristina , Kohler Mathias , Hennig Lars , Gruissem Wilhelm , Vanderschuren Hervé
Published: May 2017

Accelerated breeding of plant species has the potential to help challenge environmental and biochemical cues to support global crop security. We demonstrate the over-expression of Arabidopsis FLOWERING LOCUS T in Agrobacterium-mediated transformed cassava (Manihot esculenta Crantz; cultivar 60444) to trigger early flowering in glasshouse-grown plants. An event seldom seen in a glasshouse environment, precocious flowering and mature inflorescence were obtained within 4–5 months from planting of stem cuttings. Manual pollination using pistillate and staminate flowers from clonal propagants gave rise to viable seeds that germinated into morphologically typical progeny. This strategy comes at a time when accelerated crop breeding is of increasing importance to complement progressive genome editing techniques.
N,N-Dimethylformamide (DMF) Usage in Epoxy/Graphene Nanocomposites: Problems Associated with Reaggregation
Authors: Wei Jiacheng , Saharudin Mohd , Vo Thuc , Inam Fawad
Published: May 2017

DMF is one the most commonly-used solvents for preparing graphene nanocomposites. Various processing variables for DMF are being used for the preparation of epoxy/graphene nanocomposites. Whilst the emphasis of all of these reported studies are on the improvements in mechanical, and other properties, of the epoxy/graphene nanocomposites, there is no study investigating how DMF affects the processing and how it is associated with the final properties of the nanocomposites. In this work, different dosages of DMF have been used to prepare nanocomposites. Mechanical testing, X-ray diffraction (XRD), dynamic mechanical analysis (DMA), thermogravimetric analysis (TGA), and scanning electron microscopy (SEM) have been used to analyze the effectiveness of DMF dosage on the properties of processed nanocomposites. Larger dosages of DMF are not always ideal for dispersing graphene as it promotes reaggregation of graphene during the processing.
Special Issue “Real-Time Optimization” of Processes
Authors: Bonvin Dominique
Published: May 2017

Process optimization is the method of choice for improving the performance of industrial processes, while also enforcing the satisfaction of safety and quality constraints.[...]
Art Images in Holistic Nursing Education
Authors: Elhammoumi Cheryl , Kellam Barbara
Published: May 2017

Background: Nursing research has concentrated on empirical knowing with little focus on aesthetic knowing. Evidence from the literature suggests that using visual art in nursing education enhances both clinical observation skills and interpersonal skills. The purpose of this review was to explore how visual art has been used in baccalaureate nursing education. Methods: Of 712 records, 13 studies met the criteria of art, nursing and education among baccalaureate nursing students published in English. Results: Three quantitative studies demonstrated statistical significance between nursing students who participated in arts-based learning compared to nursing students who received traditional learning. Findings included improved recall, increased critical thinking and enhanced emotional investment. Themes identified in 10 qualitative studies included spirituality as role enhancement, empathy, and creativity. Conclusion: Visual arts-based learning in pre-licensure curriculum complements traditional content. It supports spirituality as role enhancement in nurse training. Visual art has been successfully used to enhance both critical thinking and interpersonal relations. Nursing students may experience a greater intra-connectedness that results in better inter-connectedness with patients and colleagues. Incorporating visual arts into pre-licensure curriculums is necessary to nurture holistic nursing practice.
Islam and Democracy: Conflicts and Congruence
Authors: Islam Md , Islam Md
Published: May 2017

Is authoritarianism intrinsic to Islam? Is Islam incompatible with democracy? These questions are frequently debated in the context of the study of the relationship between the Western and Islamic civilization. The debate has gained momentum since the last decade of the twentieth century, especially after the collapse of the former Soviet Union and the subsequent transition of socialist states in Eastern Europe and other authoritarian states in Asia and Latin America to democracy. The publication of The Clash of Civilizations by American scholar Samuel Huntington, in which he presented a controversial argument about a cultural divide and clash between the Islamic world and the West, pushed the debate even further. Apart from Muslim intellectuals, Western academics have spent a significant amount of time on these questions, with a multitude of articles and volumes examining the compatibility of Islam and democracy. In this paper, we will examine Islam’s relationship with democracy from normative and philosophical viewpoints, examining how the established values and principles of Islam as reflected in the Qur’anic and prophetic traditions correspond to Western democratic norms and practices. In order to obtain a profound understanding of this subject, we have delved into, through content analysis, the thoughts of several early modernist Islamic scholars who have had tremendous impact on contemporary Islamic revivalist movements throughout the world, and interviewed a number of contemporary Islamic thinkers in Bangladesh.
Evaluation and Comparison of the Processing Methods of Airborne Gravimetry Concerning the Errors Effects on Downward Continuation Results: Case Studies in Louisiana (USA) and the Tibetan Plateau (China)
Authors: Zhao Qilong , Strykowski Gabriel , Li Jiancheng , Pan Xiong , Xu Xinyu
Published: May 2017

Gravity data gaps in mountainous areas are nowadays often filled in with the data from airborne gravity surveys. Because of the errors caused by the airborne gravimeter sensors, and because of rough flight conditions, such errors cannot be completely eliminated. The precision of the gravity disturbances generated by the airborne gravimetry is around 3–5 mgal. A major obstacle in using airborne gravimetry are the errors caused by the downward continuation. In order to improve the results the external high-accuracy gravity information e.g., from the surface data can be used for high frequency correction, while satellite information can be applying for low frequency correction. Surface data may be used to reduce the systematic errors, while regularization methods can reduce the random errors in downward continuation. Airborne gravity surveys are sometimes conducted in mountainous areas and the most extreme area of the world for this type of survey is the Tibetan Plateau. Since there are no high-accuracy surface gravity data available for this area, the above error minimization method involving the external gravity data cannot be used. We propose a semi-parametric downward continuation method in combination with regularization to suppress the systematic error effect and the random error effect in the Tibetan Plateau; i.e., without the use of the external high-accuracy gravity data. We use a Louisiana airborne gravity dataset from the USA National Oceanic and Atmospheric Administration (NOAA) to demonstrate that the new method works effectively. Furthermore, and for the Tibetan Plateau we show that the numerical experiment is also successfully conducted using the synthetic Earth Gravitational Model 2008 (EGM08)-derived gravity data contaminated with the synthetic errors. The estimated systematic errors generated by the method are close to the simulated values. In addition, we study the relationship between the downward continuation altitudes and the error effect. The analysis results show that the proposed semi-parametric method combined with regularization is efficient to address such modelling problems.
Traffic Sign Detection System for Locating Road Intersections and Roundabouts: The Chilean Case
Authors: Villalón-Sepúlveda Gabriel , Torres-Torriti Miguel , Flores-Calero Marco
Published: May 2017

This paper presents a traffic sign detection method for signs close to road intersections and roundabouts, such as stop and yield (give way) signs. The proposed method relies on statistical templates built using color information for both segmentation and classification. The segmentation method uses the RGB-normalized (ErEgEb) color space for ROIs (Regions of Interest) generation based on a chromaticity filter, where templates at 10 scales are applied to the entire image. Templates consider the mean and standard deviation of normalized color of the traffic signs to build thresholding intervals where the expected color should lie for a given sign. The classification stage employs the information of the statistical templates over YCbCr and ErEgEb color spaces, for which the background has been previously removed by using a probability function that models the probability that the pixel corresponds to a sign given its chromaticity values. This work includes an analysis of the detection rate as a function of the distance between the vehicle and the sign. Such information is useful to validate the robustness of the approach and is often not included in the existing literature. The detection rates, as a function of distance, are compared to those of the well-known Viola–Jones method. The results show that for distances less than 48 m, the proposed method achieves a detection rate of 87.5 % and 95.4 % for yield and stop signs, respectively. For distances less than 30 m, the detection rate is 100 % for both signs. The Viola–Jones approach has detection rates below 20 % for distances between 30 and 48 m, and barely improves in the 20–30 m range with detection rates of up to 60 % . Thus, the proposed method provides a robust alternative for intersection detection that relies on statistical color-based templates instead of shape information. The experiments employed videos of traffic signs taken in several streets of Santiago, Chile, using a research platform implemented at the Robotics and Automation Laboratory of PUC to develop driver assistance systems.
Amorphous SiC/c-ZnO-Based Quasi-Lamb Mode Sensor for Liquid Environments
Authors: Caliendo Cinzia , Hamidullah Muhammad , Laidoudi Farouk
Published: May 2017

The propagation of the quasi-Lamb modes along a-SiC/ZnO thin composite plates was modeled and analysed with the aim to design a sensor able to detect the changes in parameters of a liquid environment, such as added mass and viscosity changes. The modes propagation was modeled by numerically solving the system of coupled electro-mechanical field equations in three media. The mode shape, the power flow, the phase velocity, and the electroacoustic coupling efficiency (K2) of the modes were calculated, specifically addressing the design of enhanced-coupling, microwave frequency sensors for applications in probing the solid/liquid interface. Three modes were identified that have predominant longitudinal polarization, high phase velocity, and quite good K2: the fundamental quasi symmetric mode (qS0) and two higher order quasi-longitudinal modes (qL1 and qL2) with a dominantly longitudinal displacement component in one plate side. The velocity and attenuation of these modes were calculated for different liquid viscosities and added mass, and the gravimetric and viscosity sensitivities of both the phase velocity and attenuation were theoretically calculated. The present study highlights the feasibility of the a-SiC/ZnO acoustic waveguides for the development of high-frequency, integrated-circuit compatible electroacoustic devices suitable for working in a liquid environment.
Mapping Winter Wheat with Multi-Temporal SAR and Optical Images in an Urban Agricultural Region
Authors: Zhou Tao , Pan Jianjun , Zhang Peiyu , Wei Shanbao , Han Tao
Published: May 2017

Winter wheat is the second largest food crop in China. It is important to obtain reliable winter wheat acreage to guarantee the food security for the most populous country in the world. This paper focuses on assessing the feasibility of in-season winter wheat mapping and investigating potential classification improvement by using SAR (Synthetic Aperture Radar) images, optical images, and the integration of both types of data in urban agricultural regions with complex planting structures in Southern China. Both SAR (Sentinel-1A) and optical (Landsat-8) data were acquired, and classification using different combinations of Sentinel-1A-derived information and optical images was performed using a support vector machine (SVM) and a random forest (RF) method. The interference coherence and texture images were obtained and used to assess the effect of adding them to the backscatter intensity images on the classification accuracy. The results showed that the use of four Sentinel-1A images acquired before the jointing period of winter wheat can provide satisfactory winter wheat classification accuracy, with an F1 measure of 87.89%. The combination of SAR and optical images for winter wheat mapping achieved the best F1 measure–up to 98.06%. The SVM was superior to RF in terms of the overall accuracy and the kappa coefficient, and was faster than RF, while the RF classifier was slightly better than SVM in terms of the F1 measure. In addition, the classification accuracy can be effectively improved by adding the texture and coherence images to the backscatter intensity data.
Automatic Detection of Driver Fatigue Using Driving Operation Information for Transportation Safety
Authors: Li Zuojin , Chen Liukui , Peng Jun , Wu Ying
Published: May 2017

Fatigued driving is a major cause of road accidents. For this reason, the method in this paper is based on the steering wheel angles (SWA) and yaw angles (YA) information under real driving conditions to detect drivers’ fatigue levels. It analyzes the operation features of SWA and YA under different fatigue statuses, then calculates the approximate entropy (ApEn) features of a short sliding window on time series. Using the nonlinear feature construction theory of dynamic time series, with the fatigue features as input, designs a “2-6-6-3” multi-level back propagation (BP) Neural Networks classifier to realize the fatigue detection. An approximately 15-h experiment is carried out on a real road, and the data retrieved are segmented and labeled with three fatigue levels after expert evaluation, namely “awake”, “drowsy” and “very drowsy”. The average accuracy of 88.02% in fatigue identification was achieved in the experiment, endorsing the value of the proposed method for engineering applications.
DeepMap+: Recognizing High-Level Indoor Semantics Using Virtual Features and Samples Based on a Multi-Length Window Framework
Authors: Zhang Wei , Zhou Siwang
Published: May 2017

Existing indoor semantic recognition schemes are mostly capable of discovering patterns through smartphone sensing, but it is hard to recognize rich enough high-level indoor semantics for map enhancement. In this work we present DeepMap+, an automatical inference system for recognizing high-level indoor semantics using complex human activities with wrist-worn sensing. DeepMap+ is the first deep computation system using deep learning (DL) based on a multi-length window framework to enrich the data source. Furthermore, we propose novel methods of increasing virtual features and virtual samples for DeepMap+ to better discover hidden patterns of complex hand gestures. We have performed 23 high-level indoor semantics (including public facilities and functional zones) and collected wrist-worn data at a Wal-Mart supermarket. The experimental results show that our proposed methods can effectively improve the classification accuracy.