bluuebunny/crossref_metadata_2025 huggingface dataset:
The Proteome of Extracellular Vesicles Released from Pulmonary Microvascular Endothelium Reveals Impact of Oxygen Conditions on Biotrauma
Authors: Schaubmayr Wolfgang , Hochreiter Beatrix , Hunyadi-Gulyas Eva , Riegler Louise , Schmidt Katy , Tiboldi Akos , Moser Bernhard , Klein Klaus U. , Krenn Katharina , Scharbert Gisela , Mohr Thomas , Schmid Johannes A. , Spittler Andreas , Tretter Verena
Published: February 2024

The lung can experience different oxygen concentrations, low as in hypoxia, high as under supplemental oxygen therapy, or oscillating during intermittent hypoxia as in obstructive sleep apnea or intermittent hypoxia/hyperoxia due to cyclic atelectasis in the ventilated patient. This study aimed to characterize the oxygen-condition-specific protein composition of extracellular vesicles (EVs) released from human pulmonary microvascular endothelial cells in vitro to decipher their potential role in biotrauma using quantitative proteomics with bioinformatic evaluation, transmission electron microscopy, flow cytometry, and non-activated thromboelastometry (NATEM). The release of vesicles enriched in markers CD9/CD63/CD81 was enhanced under intermittent hypoxia, strong hyperoxia and intermittent hypoxia/hyperoxia. Particles with exposed phosphatidylserine were increased under intermittent hypoxia. A small portion of vesicles were tissue factor-positive, which was enhanced under intermittent hypoxia and intermittent hypoxia/hyperoxia. EVs from treatment with intermittent hypoxia induced a significant reduction of Clotting Time in NATEM analysis compared to EVs isolated after normoxic exposure, while after intermittent hypoxia/hyperoxia, tissue factor in EVs seems to be inactive. Gene set enrichment analysis of differentially expressed genes revealed that EVs from individual oxygen conditions potentially induce different biological processes such as an inflammatory response under strong hyperoxia and intermittent hypoxia/hyperoxia and enhancement of tumor invasiveness under intermittent hypoxia.
Harnessing the Transcriptional Signatures of CAR-T-Cells and Leukemia/Lymphoma Using Single-Cell Sequencing Technologies
Authors: Liao Yu-Mei , Hsu Shih-Hsien , Chiou Shyh-Shin
Published: February 2024

Chimeric antigen receptor (CAR)-T-cell therapy has greatly improved outcomes for patients with relapsed or refractory hematological malignancies. However, challenges such as treatment resistance, relapse, and severe toxicity still hinder its widespread clinical application. Traditional transcriptome analysis has provided limited insights into the complex transcriptional landscape of both leukemia cells and engineered CAR-T-cells, as well as their interactions within the tumor microenvironment. However, with the advent of single-cell sequencing techniques, a paradigm shift has occurred, providing robust tools to unravel the complexities of these factors. These techniques enable an unbiased analysis of cellular heterogeneity and molecular patterns. These insights are invaluable for precise receptor design, guiding gene-based T-cell modification, and optimizing manufacturing conditions. Consequently, this review utilizes modern single-cell sequencing techniques to clarify the transcriptional intricacies of leukemia cells and CAR-Ts. The aim of this manuscript is to discuss the potential mechanisms that contribute to the clinical failures of CAR-T immunotherapy. We examine the biological characteristics of CAR-Ts, the mechanisms that govern clinical responses, and the intricacies of adverse events. By exploring these aspects, we hope to gain a deeper understanding of CAR-T therapy, which will ultimately lead to improved clinical outcomes and broader therapeutic applications.
Feasibility of a Type 2 Diabetes Prevention Program at Nationwide Level in General Practice: A Pilot Study in Italy
Authors: La Grotta Rosalba , Pellegrini Valeria , Prattichizzo Francesco , Amata Oriana , Panella Lorenzo , Frizziero Antonio , Visconti Marco , Averame Gabriella , Brasesco Pier Claudio , Calabrese Ilaria , Vaccaro Olga , Ceriello Antonio
Published: February 2024

Background: Lifestyle interventions halt the progression of prediabetes to frank type 2 diabetes (T2D). However, the feasibility of a diabetes prevention program promoting tailored interventions on a national scale and conducted by primary care physicians is unclear. Methods: General practitioners located in ten different regions throughout Italy enrolled random subjects without known metabolic diseases to identify individuals with prediabetes and prescribe them an intervention based on physical activity. Using a simple stepwise approach, people referring to their primary care physician for any reason were screened for their diabetes risk with a web-based app of the Findrisc questionnaire. Those at risk for T2D, i.e., with a Findrisc score >9, were invited to come back after overnight fasting to measure fasting glycaemia (FG). Those with 100 ≤ FG < 126 mg/dL were considered as people with prediabetes and compiled the Physical Activity Readiness Questionnaire (PAR-Q) to then receive a personalised prescription of physical activity. Results: Overall, 5928 people were enrolled and compiled the questionnaire. Of these, 2895 (48.8%) were at risk for T2D. Among these, FG was measured in 2168 subjects (participation rate 75%). The numbers of individuals with undetected prediabetes and T2D according to FG were 755 and 79 (34.8% and 3.6% of those assessing FG), respectively. Of the 755 subjects in the prediabetes range, 739 compiled the PAR-Q and started a personalised program of physical activity (participation rate 97%). Physicians involved in the study reported a mean of 6 min to perform the screening. Conclusions: Overall, these data suggest the feasibility of a national diabetes prevention program developed by general practitioners using a simple stepwise approach starting from a web app to intercept individuals with prediabetes.
The DizzyQuest Combined with Accelerometry: Daily Physical Activities and Limitations among Patients with Bilateral Vestibulopathy Due to DFNA9
Authors: Martin Erik , de Hoon Sofie , Stultiens Joost , Janssen Miranda , Essers Hans , Meijer Kenneth , Bijnens Wouter , van de Berg Maurice , Herssens Nolan , Janssens de Varebeke Sebastien , Hallemans Ann , Van Rompaey Vincent , Guinand Nils , Perez-Fornos Angelica , Widdershoven Josine , van de Berg Raymond
Published: February 2024

Background: DFNA9 is a genetic disease of the inner ear, causing progressive bilateral sensorineural deafness and bilateral vestibulopathy (BV). In this study, DizzyQuest, a mobile vestibular diary, and the MOX accelerometer were combined to assess the daily life functional limitations and physical activity of patients with DFNA9 suffering from BV. These parameters might be appropriate as potential candidacy criteria and outcome measures for new therapeutic interventions for BV. Methods: Fifteen DFNA9 patients with BV and twelve age-matched healthy controls were included. The DizzyQuest was applied for six consecutive days, which assessed the participants’ extent of functional limitations, tiredness, types of activities performed during the day, and type of activity during which the participant felt most limited. The MOX accelerometer was worn during the same six days of DizzyQuest use, measuring the participants intensity and type of physical activity. Mixed-effects linear and logistic regression analyses were performed to compare the DFNA9 patients and control group. Results: DFNA9 patients with BV felt significantly more limited in activities during the day compared to the age-matched controls, especially in social participation (p < 0.005). However, these reported limitations did not cause adjustment in the types of activities and did not reduce the intensity or type of physical activity measured with accelerometry. In addition, no relationships were found between self-reported functional limitations and physical activity. Conclusions: This study demonstrated that self-reported functional limitations are significantly higher among DFNA9 patients with BV. As a result, these limitations might be considered as part of the candidacy criteria or outcome measures for therapeutic interventions. In addition, the intensity or type of physical activity performed during the day need to be addressed more specifically in future research.
Long-Term Follow-Up of Custom-Made Porous Hydroxyapatite Cranioplasties: Analysis of Infections in Adult and Pediatric Patients
Authors: Mannella Francesca Carolina , Faedo Francesca , Fumagalli Marta , Norata Giuseppe Danilo , Zaed Ismail , Servadei Franco
Published: February 2024

In neurosurgery, cranioplasty (CP) stands as a pivotal surgical intervention, particularly following head trauma or various neurosurgical interventions. This study scrutinizes the intricacies of CP, emphasizing its prevalence and associated complications, with a specific focus on custom-made porous hydroxyapatite (PHA) implants. The investigation spans 687 patients (with 80 patients of pediatric age, less than 14 years old) across 26 neurosurgical centers in five European countries. Methodologically, this study delves into patient characteristics, complications, and infection data through a comprehensive post-marketing on-site surveillance approach. Notably, infections emerged as the primary complication, affecting 41 patients (6% of implants) with a clear distinction in onset patterns between pediatric (with more infections, 10% versus 5.4% in adults and an earlier onset of complications) and adult populations. Out of these 41 cases, cranioplasty explantation was required in 30 patients, 4.4% of the total population. Furthermore, bifrontal decompression correlated with a significantly elevated infection risk as compared to unilateral decompression (12.5% versus 5.1%) which remains after the examination of possible confounding factors. These findings provide substantial insights into the complexities of CP, suggesting the necessity for tailored strategies in pediatric and adult cases and cautioning against bifrontal decompressions. Despite acknowledging limitations and calling for prospective studies with long term follow-up, this research advances our understanding of the use of PHA CP, guiding clinical decision-making and emphasizing the importance of customized approaches for diverse patient cohorts.
Do Different Types of Microphones Affect Listening Effort in Cochlear Implant Recipients? A Pupillometry Study
Authors: Ghiselli Sara , Pizzol Erica , Vincenti Vincenzo , Fabrizi Enrico , Salsi Daria , Cuda Domenico
Published: February 2024

Background: It is known that subjects with a cochlear implant (CI) need to exert more listening effort to achieve adequate speech recognition compared to normal hearing subjects. One tool for assessing listening effort is pupillometry. The aim of this study is to evaluate the effectiveness of adaptive directional microphones in reducing listening effort for CI recipients. Methods: We evaluated listening in noise and listening effort degree (by pupillometry) in eight bimodal subjects with three types of CI microphones and in three sound configurations. Results: We found a correlation only between sound configurations and listening in noise score (p-value 0.0095). The evaluation of the microphone types shows worse scores in listening in noise with Opti Omni (+3.15 dB SNR) microphone than with Split Dir (+1.89 dB SNR) and Speech Omni (+1.43 dB SNR). No correlation was found between microphones and sound configurations and within the pupillometric data. Conclusions: Different types of microphones have different effects on the listening of CI patients. The difference in the orientation of the sound source is a factor that has an impact on the listening effort results. However, the pupillometry measurements do not significantly correlate with the different microphone types.
Prospective Study on the Correlation between CART and Leptin Gene Expression, Obesity, and Reproductive Hormones in Individuals Undergoing Bariatric Surgery
Authors: Voros Charalampos , Mpananis Kyriakos , Papapanagiotou Angeliki , Pouliakis Abraham , Mavrogianni Despoina , Mavriki Konstantina , Gkaniatsos Ioannis , Karasmani Christina , Prokopakis Ioannis , Darlas Menelaos , Anysiadou Sofia , Daskalakis George , Domali Ekaterini
Published: February 2024

Obesity, a global health concern affecting 650 million individuals of all ages worldwide, prompts health complications, including fertility issues. This research investigates the impact of bariatric surgery on morbidly obese females under 40, examining the relationship between CART and leptin gene expressions and reproductive hormones. Post-surgery, a significant reduction in BMI (16.03 kg/m2, n = 29) was observed, accompanied by notable hormonal changes. FSH levels showed a mean difference of 3.18 ± 1.19 pre- and post-surgery (p < 0.001), LH levels exhibited a mean difference of 2.62 ± 1.1 (p < 0.001), E2 levels demonstrated a mean difference of 18.62 ± 5.02 (p < 0.001), and AMH levels showed a mean difference of 3.18 ± 1.19 (p < 0.001). High CART and leptin expressions before treatment correlated with lower expressions after treatment. These findings, rooted in statistically significant correlations (CART: rs = 0.51, p = 0.005; leptin: rs = 0.75, p < 0.001), shed light on potential molecular pathways connecting gene expressions with reproductive hormones post-bariatric surgery. Our study uniquely investigates the interplay between genetic markers, infertility, and bariatric surgery in women. It stands out by providing distinctive insights into the development of personalized treatment strategies for obesity-related infertility, contributing to a deeper understanding of this complex medical issue.
The Impact of Mechanical Bowel Preparation and Oral Antibiotics in Colorectal Cancer Surgery (MECCA Study): A Prospective Randomized Clinical Trial
Authors: Frountzas Maximos , Michalopoulou Victoria , Georgiou Georgia , Kanata Despoina , Matiatou Maria , Kimpizi Despina , Matthaiou Georgia , Spiliotopoulos Spilios , Vouros Dimitrios , Toutouzas Konstantinos G. , Theodoropoulos George E.
Published: February 2024

Background: Colorectal cancer surgery has been associated with surgical site infections (SSIs), leading to an increase in postoperative morbidity, length of stay and total cost. The aim of the present randomized study was to investigate the relationship between the preoperative administration of oral antibiotic therapy and SSI rate, as well as other postoperative outcomes in patients undergoing colorectal cancer surgery. Material and Methods: Patients who underwent colorectal cancer surgery in a university surgical department were included in the present study. Patients were randomized into two groups using the “block randomization” method. The intervention group received three doses of 400 mg rifaximin and one dose of 500 mg metronidazole per os, as well as mechanical bowel preparation the day before surgery. The control group underwent only mechanical bowel preparation the day before surgery. The study has been registered in ClinicalTrials.gov (NCT03563586). Results: Two hundred and five patients were finally included in the present study, 97 of whom received preoperative antibiotic therapy per os (intervention group). Patients of this group demonstrated a significantly lower SSI rate compared with patients who did not receive preoperative antibiotic therapy (7% vs. 16%, p = 0.049). However, preoperative antibiotic administration was not correlated with any other postoperative outcome (anastomotic leak, overall complications, readmissions, length of stay). Conclusions: Preoperative antibiotic therapy in combination with mechanical bowel preparation seemed to be correlated with a lower SSI rate after colorectal cancer surgery.
Build–Launch–Consolidate Framework and Toolkit for Impact Analysis on Wireless Sensor Networks
Authors: Alghofaili Rakan , Albinali Hussah , Azzedin Farag
Published: February 2024

The Internet of Things (IoT) and wireless sensor networks (WSNs) utilize their connectivity to enable solutions supporting a spectrum of industries in different and volatile environments. To effectively enhance the security and quality of the service of networks, empirical research should consider a variety of factors and be reproducible. This will not only ensure scalability but also enable the verification of conclusions, leading to more reliable solutions. Cooja offers limited performance analysis capabilities of simulations, which are often extracted and calculated manually. In this paper, we introduce the Build–Launch–Consolidate (BLC) framework and a toolkit that enable researchers to conduct structured and conclusive experiments considering different factors and metrics, experiment design, and results analysis. Furthermore, the toolkit analyzes diverse network metrics across various scenarios. As a proof of concept, this paper studies the flooding attacks on the IoT and illustrates their impact on the network, utilizing the BLC framework and toolkit.
Study on the Spatial Arrangement of Urban Parkland under the Perspective of Equity—Taking Harbin Main City as an Example
Authors: Zhang Jun , Li Jiawei
Published: February 2024

The connotation and measurement standard of “fairness” in parkland planning is closely related to the level of urban development and residents’ needs, with solid realism and dynamics, and it is still a complex problem to control accurately in planning. Therefore, to conduct a more comprehensive study, taking the main urban area of Harbin as an example, this paper combined urban development background and practical problems at different stages and, based on POI and AOI data, divided the connotation of “equity” in park green space planning into two levels: “spatial equity” and “social equity”. Based on these two levels of fairness, the research framework of urban park green space layout is constructed. Kernel density estimation and GIS-based network analysis were used to study the spatial layout and accessibility of urban parkland. The ability to match supply and demand for parkland in the study area as a whole was analyzed using a gridded two-step moving search method, as well as the degree of equity in the enjoyment of urban parkland by vulnerable groups including the elderly and children using the Lorenz curve with the Gini coefficient, the share index, and the entropy of the locality. The results show that: (1) the parkland is concentrated in the seven main urban areas of the two–two junctions, and the cohesion distribution trend is outside the distribution. (2) Motorized vehicles have the best 15-min accessibility for travel, but it still does not cover all the parkland. (3) Supply and demand within the region are unsatisfactory, with the area with the strongest supply and demand capacity for parkland accounting for only 3.19% of the total area. (4) The Gini coefficient of parkland enjoyed by the residents of Harbin city center is 0.407, with a massive gap in the overall distribution. In addition, the share index of vulnerable groups of the elderly and children all have share indices below 1, and the results of the analysis of locational entropy indicate that the level of street park green space resources enjoyed by these two groups belongs to the low socio-spatial equity pattern, which is not balanced. This study investigates the spatial layout of urban parkland from two equity levels, which can provide new ideas for the equity study of urban green space planning.
Distribution and Utilization of Vitamin E in Different Organs of Wild Bats from Different Food Groups
Authors: Mena Canata Diego Antonio , Benfato Mara Silveira , Pereira Francielly Dias , Pereira María João Ramos , Rampelotto Pabulo Henrique
Published: February 2024

In this work, we examined the levels of vitamin E in the heart, liver, and kidneys of four species of adult male bats with distinct feeding habits. Our results indicate consistent vitamin E levels in the heart across all four bat species, suggesting the presence of regulatory mechanisms. Additionally, the liver displayed notably higher vitamin E levels in nectarivorous and frugivorous bats, while hematophagous bats exhibited lower levels, indicating a link between dietary intake and liver vitamin E levels. Furthermore, correlation analysis provided additional insights into the relationships between vitamin E and key antioxidant parameters in the livers of bats. On the other hand, no correlation was observed between vitamin E and key antioxidant parameters in the heart. Intriguingly, vitamin E was not detected in the kidneys, likely due to physiological factors and the prioritization of vitamin E mobilization in the heart, where it serves critical physiological functions. This unexpected absence of vitamin E in bat kidneys highlights the unique metabolic demands and prioritization of vitamin mobilization in wild animals like bats, compared to conventional animal models. These findings provide insight into the intricate distribution and utilization of vitamin E in bats, emphasizing the influence of dietary intake and metabolic adaptations on vitamin E levels in different organs.
The Diversity of Parasitoids and Their Role in the Control of the Siberian Moth, Dendrolimus sibiricus (Lepidoptera: Lasiocampidae), a Major Coniferous Pest in Northern Asia
Authors: Kirichenko Natalia I. , Ageev Alexander A. , Astapenko Sergey A. , Golovina Anna N. , Kasparyan Dmitry R. , Kosheleva Oksana V. , Timokhov Alexander V. , Tselikh Ekaterina V. , Zakharov Evgeny V. , Musolin Dmitrii L. , Belokobylskij Sergey A.
Published: February 2024

The Siberian moth, Dendrolimus sibiricus Tschetv., 1908 (Lepidoptera: Lasiocampidae) is a conifer pest that causes unprecedented forest mortality in Northern Asia, leading to enormous ecological and economic losses. This is the first study summarizing data on the parasitoid diversity and parasitism of this pest over the last 118 years (1905–2022). Based on 860 specimens of freshly reared and archival parasitoids, 16 species from two orders (Hymenoptera and Diptera) were identified morphologically and/or with the use of DNA barcoding. For all of them, data on distribution and hosts and images of parasitoid adults are provided. Among them, the braconid species, Meteorus versicolor (Wesmael, 1835), was documented as a parasitoid of D. sibiricus for the first time. The eastern Palaearctic form, Aleiodes esenbeckii (Hartig, 1838) dendrolimi (Matsumura, 1926), status nov., was resurrected from synonymy as a valid subspecies, and a key for its differentiation from the western Palaearctic subspecies Aleiodes esenbeckii ssp. esenbecki is provided. DNA barcodes of 11 parasitoid species from Siberia, i.e., nine hymenopterans and two dipterans, represented novel records and can be used for accurate molecular genetic identification of species. An exhaustive checklist of parasitoids accounting for 93 species associated with D. sibirisus in northern Asia was compiled. Finally, the literature and original data on parasitism in D. sibiricus populations for the last 83 years (1940–2022) were analysed taking into account the pest population dynamics (i.e., growth, outbreak, decline, and depression phases). A gradual time-lagged increase in egg and pupal parasitism in D. sibiricus populations was detected, with a peak in the pest decline phase. According to long-term observations, the following species are able to cause significant mortality of D. sibiricus in Northern Asia: the hymenopteran egg parasitoids Telenomus tetratomus and Ooencyrtus pinicolus; the larval parasitoids Aleiodes esenbeckii sp. dendrolimi, Cotesia spp., and Glyptapanteles liparidis; and the dipteran pupal parasitoids Masicera sphingivora, Tachina sp., and Blepharipa sp. Their potential should be further explored in order to develop biocontrol programs for this important forest pest.
NAFLD Fibrosis Progression and Type 2 Diabetes: The Hepatic–Metabolic Interplay
Authors: Cernea Simona
Published: February 2024

The bidirectional relationship between type 2 diabetes and (non-alcoholic fatty liver disease) NAFLD is indicated by the higher prevalence and worse disease course of one condition in the presence of the other, but also by apparent beneficial effects observed in one, when the other is improved. This is partly explained by their belonging to a multisystemic disease that includes components of the metabolic syndrome and shared pathogenetic mechanisms. Throughout the progression of NAFLD to more advanced stages, complex systemic and local metabolic derangements are involved. During fibrogenesis, a significant metabolic reprogramming occurs in the hepatic stellate cells, hepatocytes, and immune cells, engaging carbohydrate and lipid pathways to support the high-energy-requiring processes. The natural history of NAFLD evolves in a variable and dynamic manner, probably due to the interaction of a variable number of modifiable (diet, physical exercise, microbiota composition, etc.) and non-modifiable (genetics, age, ethnicity, etc.) risk factors that may intervene concomitantly, or subsequently/intermittently in time. This may influence the risk (and rate) of fibrosis progression/regression. The recognition and control of the factors that determine a rapid progression of fibrosis (or its regression) are critical, as the fibrosis stages are associated with the risk of liver-related and all-cause mortality.
Associations between Vitamin D Status and Polysomnographic Parameters in Adults with Obstructive Sleep Apnea
Authors: Kechribari Ioanna , Kontogianni Meropi D. , Georgoulis Michael , Lamprou Kallirroi , Perraki Eleni , Vagiakis Emmanouil , Yiannakouris Nikos
Published: February 2024

Vitamin D deficiency (VDD) may be associated with obstructive sleep apnea (OSA) presence and is more pronounced with increasing OSA severity; however, the relationship between these two entities remains unclear. This was a cross-sectional study among 262 adults with in-hospital-attended polysomnography-diagnosed OSA and no additional major comorbidities, aiming to explore possible associations between serum 25-hydroxyvitamin D [25(OH)D] levels and polysomnographic parameters. Data on demographics, medical history, anthropometric indices, and lifestyle habits were collected at enrolment. Serum 25(OH)D was evaluated using chemiluminescence, with VDD defined as 25(OH)D < 20 ng/mL. VDD was observed in 63% of the participants. Serum 25(OH)D correlated negatively with apnea–hypopnea index and other polysomnographic indices (all p < 0.05). In logistic regression analysis, adjusting for age, sex, smoking, body mass index, physical activity, dietary vitamin D intake, and season of blood sampling, serum 25(OH)D was associated with lower odds of severe OSA [odds ratio (95% confidence interval): 0.94 (0.90–0.98)]. In the same multivariate model, VDD was associated with ~threefold higher odds of severe OSA [2.75 (1.38–5.48)]. In stratified analyses, VDD predicted OSA severity in the group of participants ≥50 y [3.54 (1.29–9.68)] and among those with body mass index ≥ 30 kg/m2 [3.38 (1.52–7.52)], but not in the younger and non-obese adults. This study provides further evidence of an inverse association between vitamin D levels and OSA severity and underscores the importance of considering vitamin D status as a potential modifiable factor in the comprehensive management of OSA.
Hydrogen Embrittlement of Ti-Al6-V4 Alloy Manufactured by Laser Powder Bed Fusion Induced by Electrochemical Charging
Authors: Roudnicka Michaela , Kacenka Zdenek , Dvorsky Drahomir , Drahokoupil Jan , Vojtech Dalibor
Published: February 2024

The 3D printing of Ti-Al6-V4 alloy is subject to much current investigation, with Laser Beam Powder Bed Fusion (PBF-LB/M) being one of the most applied technologies. Ti-Al6-V4 alloy, despite its great material properties, is susceptible to hydrogen penetration and consequent embrittlement. The level of susceptibility to hydrogen penetration depends on the microstructural state of the alloy. In this work, we compare the effect of electrochemical charging by hydrogen on Ti-Al6-V4 alloy prepared by PBF-LB/M, either in the as-built state or annealed, and conventionally prepared alloy. At the same charging conditions, considerably different hydrogen concentrations were achieved, with the as-built 3D-printed material being the most susceptible. The changes in mechanical properties are discussed in relation to changes in microstructure, studied using microscopy, X-ray, and electron diffraction techniques.
Using Compound Neural Action Potentials for Functional Validation of a High-Density Intraneural Interface: A Preliminary Study
Authors: Kundu Aritra , Patrick Erin , Currlin Seth , Madler Ryan , Delgado Francisco , Fahmy Ahmed , Verplancke Rik , Ballini Marco , Braeken Dries , de Beeck Maaike Op , Maghari Nima , Otto Kevin J. , Bashirullah Rizwan
Published: February 2024

Compound nerve action potentials (CNAPs) were used as a metric to assess the stimulation performance of a novel high-density, transverse, intrafascicular electrode in rat models. We show characteristic CNAPs recorded from distally implanted cuff electrodes. Evaluation of the CNAPs as a function of stimulus current and calculation of recruitment plots were used to obtain a qualitative approximation of the neural interface’s placement and orientation inside the nerve. This method avoids elaborate surgeries required for the implantation of EMG electrodes and thus minimizes surgical complications and may accelerate the healing process of the implanted subject.
An Overview of the Recent Advances in Pool Boiling Enhancement Materials, Structrure, and Devices
Authors: Pereira José , Souza Reinaldo , Lima Rui , Moreira António , Moita Ana
Published: February 2024

This review attempts to provide a comprehensive assessment of recent methodologies, structures, and devices for pool boiling heat transfer enhancement. Several enhancement approaches relating to the underlying fluid route and the capability to eliminate incipient boiling hysteresis, augment the nucleate boiling heat transfer coefficient, and improve the critical heat flux are assessed. Hence, this study addresses the most relevant issues related to active and passive enhancement techniques and compound enhancement schemes. Passive heat transfer enhancement techniques encompass multiscale surface modification of the heating surface, such as modification with nanoparticles, tunnels, grooves, porous coatings, and enhanced nanostructured surfaces. Also, there are already studies on the employment of a wide range of passive enhancement techniques, like displaced enhancement, swirl flow aids, and bi-thermally conductive surfaces. Moreover, the combined usage of two or more enhancement techniques, commonly known as compound enhancement approaches, is also addressed in this survey. Additionally, the present work highlights the existing scarcity of sufficiently large available databases for a given enhancement methodology regarding the influencing factors derived from the implementation of innovative thermal management systems for temperature-sensitive electronic and power devices, for instance, material, morphology, relative positioning and orientation of the boiling surface, and nucleate boiling heat transfer enhancement pattern and scale. Such scarcity means the available findings are not totally accurate and suitable for the design and implementation of new thermal management systems. The analysis of more than 100 studies in this field shows that all such improvement methodologies aim to enhance the nucleate boiling heat transfer parameters of the critical heat flux and nucleate heat transfer coefficient in pool boiling scenarios. Finally, diverse challenges and prospects for further studies are also pointed out, aimed at developing important in-depth knowledge of the underlying enhancement mechanisms of such techniques.
Consideration of Power Transmission Characteristics in a Micro-Gear Train
Authors: Arai Yasuhiko
Published: February 2024

Characteristics related to power transmission in the micro-domain, based on dry rolling contact of the gears, were investigated using a 3D-printed gear train with a pitch circle diameter of 84 µm in order to experimentally compare the power transmission efficiency in the macro- and micro-domains. For a basic gear train with two intermeshing gears, it was shown that the gear train in the micro-domain was capable of transmitting power to the same extent as in the macro-domain. However, in gear trains with complex power transmission paths, assuming a planetary gear train with multiple meshing gears, it has been shown that the power transmission characteristics of micro-domain gears differ from those in the macro-domain. The use of gear trains in the micro-region necessitates consideration of the loss of transmitted torque due to contact between tooth surfaces, which is unique to the micro-region and different from its use in the macro-region.
Enrichment Factors and Metallogenic Models of Critical Metals in Late Permian Coal Measures from Yunnan, Guizhou, and Guangxi Provinces
Authors: Cao Bo , Fu Xuehai , Kang Junqiang , Tang Pan , Xu Hui
Published: February 2024

The Late Permian coal measures in eastern Yunnan, western Guizhou, and central Guangxi are significantly enriched in critical metals that could serve as important supplements to conventional critical metal deposits in China. This study collected previous geochronological and geochemical data from the Late Permian coal measures to evaluate the distribution characteristics and enrichment factors of critical metals. Moreover, metallogenic models for critical metals were also developed. The results showed that Late Permian coal measures in Yunnan, Guizhou, and Guangxi provinces exhibited abnormal enrichment in Nb, Zr, and rare earth elements (REY, or REE if Y is excluded). The Emeishan mafic rocks and intermediate-felsic volcanic ash from the Truong Son orogenic belt underwent chemical weathering, with Nb and Zr selectively preserved in situ in the form of heavy minerals (e.g., rutile, zircon, and anatase), which subsequently led to the enrichment of Nb and Zr in bauxite and Al-claystone at the bottom of the Late Permian coal measures. Intermediate-felsic volcanic ash from the Emeishan large igneous province (ELIP) and the Truong Son orogenic belt supplied Nb, Zr, and REY for the middle and upper parts of the Late Permian coal measures. The intermediate-felsic mineral material of the coal measures in the intermediate zone, outer zone, and outside zone of ELIP are derived mainly from the ELIP, the mixture from ELIP and the Truong Son orogenic belt, and the Truong Son orogenic belts, respectively. Nb, Zr, and REY were leached by acidic aqueous solutions and from the parting and roof into underlying coal seams, where they deposited as authigenic minerals or adsorbed ions on organic matter during early coalification.
Quarter-Wave Plate Metasurfaces for Generating Multi-Channel Vortex Beams
Authors: Zhang Ziheng , Gu Manna , Cui Guosen , Zhou Yuxiang , Ma Teng , Zhao Kaixin , Li Yunxiao , Liu Chunxiang , Cheng Chuanfu , Ma Li
Published: February 2024

Metasurfaces of quarter-wave plate (QWP) meta-atoms have exhibited high flexibility and versatile functionalities in the manipulation of light fields. However, the generation of multi-channel vortex beams with the QWP meta-atom metasurfaces presents a significant challenge. In this study, we propose dielectric metasurfaces composed of QWP meta-atoms to manipulate multi-channel vortex beams. QWP meta-atoms, systematically arranged in concentric circular rings, are designed to introduce the modulations via the propagation phase and geometric phase, leading to the generation of co- and cross-polarized vortex beams in distinct channels. Theoretical investigations and simulations are employed to analyze the modulation process, confirming the capability of QWP meta-atom metasurfaces for generating the multi-channel vortex beams. This study presents prospective advancements for the compact, integrated, and multifunctional nanophotonic platforms, which have potential applications in classical physics and quantum domains.
Classifying the Role of Surface Ligands on the Passivation and Stability of Cs2NaInCl6 Double Perovskite Quantum Dots
Authors: Tosa Keita , Ding Chao , Chen Shikai , Hayase Shuzi , Shen Qing
Published: February 2024

Cs2NaInCl6 double perovskites, which have excellent photoelectric conversion properties and are non-toxic and lead-free, have recently gained significant attention. In particular, double-perovskite quantum dots (QDs) are viewed as a promising material for optoelectronic device applications. Ligands such as oleic acid (OA) and oleylamine (OAm) are essential for the synthesis of perovskite QDs, but their specific roles in double-perovskite QDs remain unclear. In this study, we have investigated the binding of OA and OAm to Cs2NaInCl6 QDs through FTIR and NMR and their effects on the surface defect reduction and stability improvement for Cs2NaInCl6 QDs. We found that only OAm was bound to the QD surfaces while OA was not. The OAm has a significant effect on the photoluminescence quantum yield (PLQY) improvement by passivating the QD surface defects. The stability of the QDs was also evaluated, and it was observed that OA played a significant role in the stability of the QDs. Our findings provide valuable insights into the roles of ligands in influencing the photophysical properties and stability of lead-free double-perovskite QDs.
A Watt-Level Pulsed Er:Lu2O3 Laser Based on a TiB2 Saturable Absorber
Authors: Liang Yangyang , Zhang Lu
Published: February 2024

TiB2 nanoparticles with a bandgap of 0 eV were prepared, and the corresponding nonlinear optical response at 2.85 μm was investigated. Employing a TiB2 as a saturable absorber, a 2.85 μm pulsed Er:Lu2O3 crystal laser with an average output power of 1.2 W was achieved under a maximum pump power of 9.51 W. Laser pulses with durations of ~203 ns were delivered at a repetition rate of 154 kHz, which corresponds to a pulse energy of ~7.8 µJ and a peak power of 39.3 W. As far as we know, the result represents the highest average output power from all Q-switched Er:Lu2O3 crystal lasers.
Synthesis and Study of the Structure–Activity Relationship of Antiproliferative N-Substituted Isosteviol-Based 1,3-Aminoalcohols
Authors: Ozsvár Dániel , Bózsity Noémi , Zupkó István , Szakonyi Zsolt
Published: February 2024

Starting from isosteviol, a series of diterpenoid 1,3-aminoalcohol derivatives were prepared via stereoselective transformations. The acid-catalysed hydrolysis and rearrangement of natural stevioside produced isosteviol, which was transformed into the key intermediate methyl ester. In the next step, an 1,3-aminoalcohol library was prepared by the reductive amination of the intermediate 3-hydroxyaldehyde obtained from isosteviol in a two-step synthesis. To study the effect of the carboxylate ester function at position 4, the free carboxylic acid, benzyl ester and acryloyl ester analogues were prepared as elongated derivatives in comparison with our earlier results in this field. The antiproliferative activity of compounds against human tumour cell lines (A2780, HeLa, MCF-7 and MDA-MB-231) was investigated. In our preliminary study, the 1,3-aminoalcohol function with N-benzyl or (1H-imidazol-1-yl)-propyl substitution and benzyl ester moiety seemed essential for the reliable antiproliferative activity. The results obtained could be a good starting point to further functionalisation towards more efficient antiproliferative diterpenes.
Pharmacological Topical Therapy for Intra-Oral Post Traumatic Trigeminal Neuropathic Pain: A Comprehensive Review
Authors: Sharav Yair , Heiliczer Shimrit , Benoliel Rafael , Haviv Yaron
Published: February 2024

Background: The efficacy of topical treatments in alleviating neuropathic pain is well-established. However, there is a paucity of research on topical interventions designed specifically for intra-oral application, where the tissue composition differs from that of exposed skin. Methods: This comprehensive review endeavors to assess the extant evidence regarding the efficacy of topical treatments in addressing neuropathic pain within the oral cavity. Utilizing combinations of search terms, we conducted a thorough search across standard electronic bibliographic databases—MEDLINE (via PubMed), Embase, Google Scholar, and Up to Date. The variables under scrutiny encompassed topical treatment, local intervention, chronic oral and orofacial pain, and neuropathic pain. All pertinent studies published in the English language between 1992 and 2022 were included in our analysis. Results: Fourteen relevant manuscripts were identified, primarily consisting of expert opinions and case reports. The comprehensive review suggests that topical treatments, especially when applied under a stent, could be effective in mitigating neuropathic pain in the oral area. However, it is crucial to conduct further studies to confirm these preliminary results. The limitations of the reviewed studies, mainly the reliance on expert opinions, small sample sizes, inconsistent study designs, and a lack of long-term follow-up data, highlight the need for more rigorous research. Conclusions: Although initial findings indicate topical treatments may be effective for oral neuropathic pain, the limitations of current studies call for more thorough research. Further comprehensive studies are essential to validate the efficacy of these treatments, standardize procedures, and determine long-term results. This will provide clearer guidance for treating chronic neuropathic pain in the oral cavity.
A Comprehensive Physiologically Based Pharmacokinetic Model of Nadolol in Adults with Renal Disease and Pediatrics with Supraventricular Tachycardia
Authors: Kalsoom Samia , Rasool Muhammad Fawad , Imran Imran , Saeed Hamid , Ahmad Tanveer , Alqahtani Faleh
Published: February 2024

Nadolol is a long-acting non-selective β–adrenergic antagonist that helps treat angina and hypertension. The current study aimed to develop and validate the physiologically based pharmacokinetic model (PBPK) of nadolol in healthy adults, renal-compromised, and pediatric populations. A comprehensive PBPK model was established by utilizing a PK-Sim simulator. After establishing and validating the model in healthy adults, pathophysiological changes i.e., blood flow, hematocrit, and GFR that occur in renal failure were incorporated in the developed model, and the drug exposure was assessed through Box plots. The pediatric model was also developed and evaluated by considering the renal maturation process. The validation of the models was carried out by visual predictive checks, calculating predicted to observed (Rpre/obs) and the average fold error (AFE) of PK parameters i.e., the area under the concentration–time curve (AUC0-t), the maximum concentration in plasma (Cmax), and CL (clearance). The presented PBPK model successfully simulates the nadolol PK in healthy adults, renal-impaired, and pediatric populations, as the Rpre/obs values of all PK parameters fall within the acceptable range. The established PBPK model can be useful in nadolol dose optimization in patients with renal failure and children with supraventricular tachycardia.
The Necessity to Investigate In Vivo Fate of Nanoparticle-Loaded Dissolving Microneedles
Authors: Chang Ziyao , Wu Yuhuan , Hu Ping , Jiang Junhuang , Quan Guilan , Wu Chuanbin , Pan Xin , Huang Zhengwei
Published: February 2024

Transdermal drug delivery systems are rapidly gaining prominence and have found widespread application in the treatment of numerous diseases. However, they encounter the challenge of a low transdermal absorption rate. Microneedles can overcome the stratum corneum barrier to enhance the transdermal absorption rate. Among various types of microneedles, nanoparticle-loaded dissolving microneedles (DMNs) present a unique combination of advantages, leveraging the strengths of DMNs (high payload, good mechanical properties, and easy fabrication) and nanocarriers (satisfactory solubilization capacity and a controlled release profile). Consequently, they hold considerable clinical application potential in the precision medicine era. Despite this promise, no nanoparticle-loaded DMN products have been approved thus far. The lack of understanding regarding their in vivo fate represents a critical bottleneck impeding the clinical translation of relevant products. This review aims to elucidate the current research status of the in vivo fate of nanoparticle-loaded DMNs and elaborate the necessity to investigate the in vivo fate of nanoparticle-loaded DMNs from diverse aspects. Furthermore, it offers insights into potential entry points for research into the in vivo fate of nanoparticle-loaded DMNs, aiming to foster further advancements in this field.
Intranasal Delivery of Anti-Apoptotic siRNA Complexed with Fas-Signaling Blocking Peptides Attenuates Cellular Apoptosis in Brain Ischemia
Authors: Chung Kunho , Ullah Irfan , Yi Yujong , Kang Eunhwa , Yun Gyeongju , Heo Seoyoun , Kim Minkyung , Chung Seong-Eun , Park Seongjun , Lim Jaeyeoung , Lee Minhyung , Rhim Taiyoun , Lee Sang-Kyung
Published: February 2024

Ischemic stroke-induced neuronal cell death leads to the permanent impairment of brain function. The Fas-mediating extrinsic apoptosis pathway and the cytochrome c-mediating intrinsic apoptosis pathway are two major molecular mechanisms contributing to neuronal injury in ischemic stroke. In this study, we employed a Fas-blocking peptide (FBP) coupled with a positively charged nona-arginine peptide (9R) to form a complex with negatively charged siRNA targeting Bax (FBP9R/siBax). This complex is specifically designed to deliver siRNA to Fas-expressing ischemic brain cells. This complex enables the targeted inhibition of Fas-mediating extrinsic apoptosis pathways and cytochrome c-mediating intrinsic apoptosis pathways. Specifically, the FBP targets the Fas/Fas ligand signaling, while siBax targets Bax involved in mitochondria disruption in the intrinsic pathway. The FBP9R carrier system enables the delivery of functional siRNA to hypoxic cells expressing the Fas receptor on their surface—a finding validated through qPCR and confocal microscopy analyses. Through intranasal (IN) administration of FBP9R/siCy5 to middle cerebral artery occlusion (MCAO) ischemic rat models, brain imaging revealed the complex specifically localized to the Fas-expressing infarcted region but did not localize in the non-infarcted region of the brain. A single IN administration of FBP9R/siBax demonstrated a significant reduction in neuronal cell death by effectively inhibiting Fas signaling and preventing the release of cytochrome c. The targeted delivery of FBP9R/siBax represents a promising alternative strategy for the treatment of brain ischemia.