bluuebunny/crossref_metadata_2025 huggingface dataset:
XRT-04. THE ROLE OF STEREOTACTIC RADIOSURGERY FOR MENINGIOMAS IN PEDIATRIC PATIENTS
Authors: Shanahan Regan , Wei Zhishuo , Miller Tracy , Deng Hansen , Niranjan Ajay , Dade Lunsford L
Published: June 2023

Despite the generally benign and slow-growing nature, meningiomas in pediatric populations often exhibit more aggressive behavior. Stereotactic radiosurgery (SRS) is a non-invasive modality that is widely used as an upfront or adjuvant treatment for patients with meningiomas. Due to the rarity of meningiomas in pediatric patients, the outcomes of SRS for childhood meningiomas have never been described. From 1987 to 2022, a total of 2,030 patients with meningiomas underwent SRS at the University of Pittsburgh Medical Center. Nine patients (2 female; incidence rate = 0.44%) with a median age of 14.5 years (range, 9—17 years) at SRS were identified. Three patients (33.3%) had a history of total body irradiation. Seven patients (77.8%) were asymptomatic at the time of SRS presentation. Seven patients (77.8%) had a pre-SRS craniotomy; two patients (22.2%) had pre-SRS fractionated radiotherapy. The median cumulative tumor volume was 3.65 cc (range, 0.01—11.84 cc). The median margin dose prescribed was 14 Gy (range, 11—25 Gy). The median follow-up time was 107 months (range, 24—194 months). Five patients (55.6%) experienced tumor progression at a median of 25 months (range, 13—161 months). Local tumor control at 1-, 5-, and 10 years was 100%, 55.56%, and 55.56%, respectively. After local tumor progression, 2 patients received repeat SRS, 2 patients had surgical resection and 1 was placed on hydroxyurea for an unresectable tumor. At the date of the last clinical follow-up, 5 patients (55.6%) were still alive while 4 patients (44.4%) expired due to the progression of intracranial disease. 1-, 5-, and 10-year survival was 100%, 88.89%, and 64.81%, respectively. Childhood meningioma is a rare tumor that exhibits aggressive behaviors. SRS should be considered as an upfront or adjuvant treatment option in the multi-modal management of childhood meningiomas for optimal tumor control and maximal quality of life preservation.
XRT-06. RAPID PTEFB-DEPENDENT TRANSCRIPTIONAL REORGANIZATION UNDERPINS THE GLIOMA ADAPTIVE RESPONSE TO RADIOTHERAPY
Authors: Walker Faye , Sobral Lays Martin , Danis Etienne , Sanford Bridget , Balakrishnan Ilango , Wang Dong , Pierce Angela , Karam Sana , Serkova Natalie , Foreman Nicholas , Venkataraman Sujatha , Dowell Robin , Vibhakar Rajeev , Dahl Nathan
Published: June 2023

Dynamic regulation of gene expression is fundamental for cellular adaptation to exogenous stressors. PTEFb-mediated pause-release of RNA polymerase II (Pol II) is a conserved regulatory mechanism for synchronous transcriptional induction in response to heat shock, but this pro-survival role has not been examined in the applied context of cancer therapy. Using a combination of ChIP-, ATAC-, and RNA-seq in model systems of diffuse intrinsic pontine glioma and other pediatric high-grade gliomas, we observe a rapid genome-wide reorganization of active chromatin within hours of exposure to therapeutic ionizing radiation. This redistribution facilitates PTEFb-mediated pause-release of Pol II to drive nascent transcriptional induction of canonical DNA damage response programs. Concurrent inhibition of PTEFb imparts a transcription elongation defect, disrupting this chromatin reorganization and blunting transcriptional induction. Functionally, concurrent PTEFb inhibition abrogates key adaptive programs such as DNA repair and cell cycle regulation. This combination demonstrates a potent, synergistic therapeutic potential agnostic of glioma subtype, leading to a marked induction of tumor cell apoptosis and prolongation of xenograft survival. These studies reveal a central role for PTEFb underpinning the early adaptive response to radiotherapy, opening new avenues for combinatorial treatment in these lethal malignancies.
TRLS-02. FEASIBILITY OF EVALUATING ABEMACICLIB NEUROPHARMACOKINETICS OF DIFFUSE MIDLINE GLIOMA USING INTRATUMORAL MICRODIALYSIS
Authors: Jackson Sadhana , Glod John , Peer Cody , Figg William , Dalmage Mahalia , Brown Desmond
Published: June 2023

BACKGROUND

Diffuse midline gliomas (DMG) are the most aggressive brain tumors of childhood and young adults, with documented 2-year survival rates <10%. Treatment failure is due in part to the function of the BBB. Intratumoral microdialysis sampling is an effective tool to determine brain entry of varied agents and could help to provide a better understanding of the relationship of drug permeability to DMG treatment response.

METHODS

This is a non-randomized, single-center, phase 1 clinical trial. Up to seven young adult (18-39 years) patients with recurrent high-grade or diffuse midline glioma will be enrolled with the goal of 5 patients completing the trial over an anticipated 24 months. All patients will take abemaciclib pre-operatively for 4.5 days at twice daily dosing. Patients will undergo resection or biopsy, placement of a microdialysis catheter, and 48 hours of dialysate sampling coupled with timed plasma collections. If intratumoral tumor or brain dialysate sampling concentrations are >10nmol/L, or tumor tissue studies demonstrate CDK inhibition, then restart of abemaciclib therapy along with temozolomide will be administered for maintenance therapy and discontinued with evidence of radiologic or clinical disease progression.

DISCUSSION

The poor survival associated with diffuse midline gliomas underscore the need for improved means to evaluate efficacy of drug delivery to tumor and peritumoral tissue. The findings of this novel study, will provide real-time measurements of BBB function which have the potential to influence future prognostic and diagnostic decisions in such a lethal disease with limited treatment options.

TRIAL REGISTRATION: Clinicaltrials.gov, NCT05413304. Registered June 10, 2022, Abemaciclib Neuropharmacokinetics of Diffuse Midline Glioma Using Intratumoral Microdialysis; https://clinicaltrials.gov/ct2/show/NCT05413304?term=22c0003&draw=2&rank=1
TRLS-08. EXPERIENCE WITH THE SELECTIVE FIBROBLAST GROWTH FACTOR INHIBITOR ERDAFITINIB IN THREE PEDIATRIC PATIENTS WITH CNS TUMORS
Authors: Stepien Natalia , Mayr Lisa , Hedrich Cora , Lippolis Maria Aliotti , Schned Hannah , Baumgartner Alicia , Raimann Adalbert , Schmook Maria-Theresa , Traub-Weidinger Tatjana , Dorfer Christian , Hartmann Gabriele , Senfter Daniel , Madlener Sibylle , Peyrl Andreas , Azizi Amedeo A , Haberler Christine , Müllauer Leonhard , Gojo Johannes
Published: June 2023

Activation of FGF signaling has been described for at least 3% of pediatric solid tumors including pediatric gliomas. Therapeutic actionability of FGF signaling activations, however, remains challenging. Here, we report on three patients with FGFR activation treated with erdafitinib, originally approved for the treatment of FGFR3 or FGFR2 mutated urothelial carcinoma. Our patients were treated for posterior fossa group A ependymoma (patient (Pat) 1&2) and glioma NOS °II-III (Pat3). Dose of erdafitinib at initiation of therapy was 5,2mg/kg/d; 3,8mg/kg/d and 2,8mg/kg/d for Pat1-3 respectively. Rationale for treatment with erdafitinib was increased FGFR3 expression in tumor tissue (TT) of Pat1, increased FGFR1 expression in TT of Pat2, and in Pat3 an activating FGFR1 internal tandem duplication (ITD). Both ependymoma patients received erdafitinib as a fourth line treatment and progressed rapidly under therapy. Treatment was discontinued after 2 weeks (Pat2) and 3 months (Pat1) due to progressive disease. Pat3, with the FGFR1 ITD, responded very well to therapy with a decrease in tumor size and reduced contrast-enhancement after 3&6 months. Therapy was discontinued after six months due to dystrophic nail changes. During treatment, our patients reported on diarrhea, abdominal pain, pain in the lower extremities, dysgeusia, hypersensitivity of the oral mucosa, dry skin and dystrophic nail changes. Additionally, we observed a distinct increase in longitudinal growth (Pat1&3), increased serum phosphate levels, LFPs, alpha-amylase, cholinesterase, alkaline phosphatase and LDH. Summing up, treatment with erdafitinib has high potential for a selected cohort of pediatric CNS tumor patients, as demonstrated by the treatment response of Pat3 with the FGFR1 IDT. However, further defining the exact molecular alterations that predict the individual patient’s response as well as side effects in the pediatric population are imperative to elucidate the further role of erdafitinib in treatment of pediatric CNS tumors.
TRLS-14. TARGETING REPLICATIVE STRESS IN BRAIN TUMORS BY COMBINING ATR AND PARP INHIBITION
Authors: Sarvode Supriya , Refaat Alaa , DeVries Rebekah , Pal Anasuya , Tinkle Christopher , Shelat Anang
Published: June 2023

Pediatric brain tumors like MYC-amplified Medulloblastomas (MB) and Atypical Teratoid and Rhabdoid Tumors (AT/RT) continue to have poor survival rates despite recent advances in understanding tumor biology. MYC is an oncogenic transcription factor often expressed in MB and AT/RT at high levels. While direct inhibition of MYC by small molecules is an active area of research, such approaches lack clinical validation and are not yet actionable. Strategies to target MYC-driven brain tumors that are immediately translatable are urgently needed. Replication stress has been identified as a prominent source of tumorigenesis over the past decade and is a potential target for cancer therapy. Specific oncogenes induce elevated levels of replicative stress through aberrant activation of transcription and dysregulation of DNA replication. In these instances, replicative stress could be rescued by either metabolic supplementation or MYC expression. The activation of ATR and PARP are critical events in response to replicative stress. PARP inhibitors stall replication forks and promote the generation of single-strand DNA, which activates ATR. Genetic and pharmacological evidence suggests the sensitization of MYC-induced tumors to PARP inhibitors. Preclinical studies in diverse tumor backgrounds have demonstrated that the combination of ATR and PARP inhibition is synergistic and leads to premature entry into mitosis, enhanced replicative stress and cell death, and tumor regression. Additionally, a reduction in ATR activity can be lethal in oncogene-induced replication stress. Using high throughput drug screening across MB and AT/RT cell lines, we found that the CNS penetrant PARP inhibitor AZD9754 significantly potentiated the cytotoxicity of ATR inhibition. This emerging synergistic combination therapy reveals new therapeutic targets in MYC-driven replicative stress. Studies are ongoing to validate in vitro findings in vivo.
Jacob’s Room
Authors: Woolf Virginia
Published: June 2023

Who is Jacob Flanders? Virginia Woolf’s third novel, published in 1922 alongside James Joyce’s Ulysses and T.S. Eliot’s The Waste Land, follows this elusive title character from a sunlit childhood on the Cornwall coast to adventures in Cambridge, London, and Athens. Women fall in love with Jacob; young men desire his company and conversation. But Woolf keeps her scornful, charming protagonist at a distance, enveloping Jacob in mystery as he enters adulthood and the Great War thunders across Europe. A daring work that reimagines every element of the traditional novel, Jacob’s Room tells a new story for a new century.

In 1922, Lytton Strachey pronounced Jacob’s Room ‘a most wonderful achievement—more like poetry, it seems to me, than anything else, and as such I prophesy immortal.’ One hundred years after its publication, Woolf’s first full-length work of experimental fiction pulls us into the inexhaustible mysteries of intimacy and mortality.
The Duel
Authors: Conrad Joseph
Published: June 2023

I

Napoleon I.,* whose career had the quality of a duel against the whole of Europe, disliked duelling between the officers of his army. The great military emperor was not a swashbuckler, and had little respect for tradition.

Nevertheless, a story of duelling, which...
V
Authors: Wharton Edith
Published: June 2023

There had never been such a June in Eagle County. Usually it was a month of moods, with abrupt alternations of belated frost and midsummer heat; this year, day followed day in a sequence of temperate beauty. Every morning a breeze blew steadily from...
VI
Authors: Wharton Edith
Published: June 2023

That evening after supper Charity sat alone in the kitchen and listened to Mr. Royall

and young Harney talking in the porch.

She had remained indoors after the table had been cleared and old Verena had hobbled

up to bed. The kitchen window was open,...
IX
Authors: Wharton Edith
Published: June 2023

Charity sat before the mirror trying on a hat which Ally Hawes, with much secrecy, had trimmed for her. It was of white straw, with a drooping brim and a cherry-coloured lining that made her face glow like the inside of the shell on...
XIII
Authors: Wharton Edith
Published: June 2023

The Town Hall was crowded and exceedingly hot. As Charity marched into it, third in the white muslin file headed by Orma Fry, she was conscious mainly of the brilliant effect of the wreathed columns framing the green-carpeted stage toward which she was moving,...
The Scarlet Pimpernel
Authors: Orczy Baroness
Published: June 2023

Sir Percy Blakeney lives a double life in the England of 1792: at home he is an idle fop and a leader of fashion, but in abroad he is the Scarlet Pimpernel, a master of disguise who saves aristocrats from the guillotine. When the revolutionary French state seeks to unmask him, Percy’s estranged, independent wife, Marguerite, unwittingly sets their agent on her husband’s track. Percy’s escapades, and Marguerite’s daring journey to France to save him from the guillotine, keep the reader turning the pages of Baroness Orczy’s well-paced romantic adventure.

Written in just five weeks in 1903, Baroness Emma Orczy’s bestseller has been the basis of multiple adaptations. Rooted in the upheaval of Orczy’s Hungarian childhood, and in the anxious nationalism of turn-of-the-century Britain, the story of the Scarlet Pimpernel provided a blueprint not only for subsequent historical swashbucklers, but for superheroes from Zorro to Superman.

The edition places the book The Scarlet Pimpernel within the context of the elite and popular literature of the turn of the century. Orczy’s novel is close in kin to such contemporary political thrillers as Joseph Conrad’s The Secret Agent (1907); tales that channelled contemporary concerns about refugees and enemies within.
Blue & Green
Authors: Woolf Virginia
Published: June 2023

Green

The pointed fingers of glass hang downwards. The light slides down the glass, and drops a pool of green. All day long the ten fingers of the lustre* drop green upon the marble. The feathers of parakeets — their harsh cries — sharp blades of...
The String Quartet
Authors: Woolf Virginia
Published: June 2023

Well, here we are, and if you cast your eye over the room you will see that Tubes and trams and omnibuses, private carriages not a few, even, I venture to believe, landaus with bays* in them, have been busy at it,...
The New Dress
Authors: Woolf Virginia
Published: June 2023

Mabel had her first serious suspicion that something was wrong as she took her cloak off and Mrs Barnet, while handing her the mirror and touching the brushes and thus drawing her attention, perhaps rather markedly, to all the appliances for tidying and improving hair,...
The Trial
Authors: Kafka Franz
Published: June 2023

A successful professional man wakes up one morning to find himself under arrest for an offence which is never explained. The mysterious court which conducts his trial is outwardly co-operative, but capable of horrific violence. Faced with this ambiguous authority, Josef K. gradually succumbs to its psychological pressure. He consults various advisers without escaping his fate. Was there some way out that he failed to see? Kafka’s unfinished novel has been read as a study of political power, a pessimistic religious parable, or a crime novel where the accused man is himself the problem.

One of the iconic figures of modern world literature, Kafka writes about universal problems of guilt, responsibility, and freedom; he offers no solutions, but provokes his readers to arrive at meanings of their own. This new edition includes the fragmentary chapters that were omitted from the main text, in a translation that is both natural and exact, and an introduction that illuminates the novel and its author.
The Metamorphosis and Other Stories
Authors: Kafka Franz
Published: June 2023

With a bewildering blend of the everyday and the fantastical, Kafka thus begins his most famous short story, The Metamorphosis. A commercial traveller is unexpectedly freed from his dreary job by his inexplicable transformation into an insect, which drastically alters his relationship with his family. Kafka considered publishing it with two of the stories included here in a volume to be called Punishments. The Judgement also concerns family tensions, when a power struggle between father and son ends with the father passing an enigmatic judgement on the helpless son. The third story, In the Penal Colony, explores questions of power, justice, punishment, and the meaning of pain in a colonial setting.

These three stories are flanked by two very different works. Meditation, the first book Kafka published, consists of light, whimsical, often poignant mood-pictures, while in the autobiographical Letter to his Father, Kafka analyses his difficult relationship in forensic and devastating detail.
A Portrait of the Artist as a Young Man
Authors: Joyce James
Published: June 2023

‘Once upon a time and a very good time it was there was a moocow coming down along the road and this moocow that was coming down along the road met a nicens little boy named baby tuckoo’

So begins one of the most significant literary works of the twentieth century, and one of the most innovative. Its originality shocked contemporary readers on its publication in 1916 who found its treating of the minutiae of daily life indecorous, and its central character unappealing. Was it art or was it filth?

The novel charts the intellectual, moral, and sexual development of Stephen Dedalus, from his childhood listening to his father’s stories through his schooldays and adolescence to the brink of adulthood and independence, and his awakening as an artist. Growing up in a Catholic family in Dublin in the final years of the nineteenth century, Stephen’s consciousness is forged by Irish history and politics, by Catholicism and culture, language and art. Stephen’s story mirrors that of Joyce himself, and the novel is both startlingly realistic and brilliantly crafted.

For this edition Jeri Johnson, editor of the acclaimed Ulysses 1922 text, has written an introduction and notes which together provide a comprehensive and illuminating appreciation of Joyce's artistry.
14
Authors: Woolf Virginia
Published: June 2023

(Certainly, Nancy had gone with them, since Minta Doyle had asked it with her dumb look, holding out her hand, as Nancy made off, after lunch, to her attic, to escape the horror of family life. She supposed she must go then. She...
Southern Journey: The Barrier Stage
Authors: Scott Robert Falcon
Published: June 2023

November 1.—Last night we heard that Jehu had reached Hut Point in about 5½ hours. This morning we got away in detachments—Michael, Nobby, Chinaman were first to get away about 11 a.m. The little devil Christopher was harnessed with the usual difficulty...
The Return From the Pole
Authors: Scott Robert Falcon
Published: June 2023

Friday, January 19.—Lunch 8.1, T. -22.6°. Early in the march we picked up a Norwegian cairn and our outward tracks. We followed these to the ominous black flag which has first apprised us of our predecessors’ success. We have picked this flag up, using...
The Last March
Authors: Scott Robert Falcon
Published: June 2023

Sunday, February 18.—R. 32. Temp. -5.5°. At Shambles Camp. We gave ourselves 5 hours’ sleep at the lower glacier depôt after the horrible night, and came on at about 3 to-day to this camp, coming fairly easily over the divide. Here with plenty of...
The Thirty-Nine Steps
Authors: Buchan John
Published: June 2023

John Buchan wrote The Thirty-Nine Steps while he was seriously ill at the beginning of the First World War. In it he introduces his most famous hero, Richard Hannay, who, despite claiming to be an `ordinary fellow’, is caught up in the dramatic race against a plot to devastate the British war effort. Hannay is hunted across the Scottish moors by police and spy-ring alike, and must outwit his intelligent and pitiless enemy in the corridors of Whitehall and, finally, at the site of the mysterious thirty-nine steps.

The best-known of Buchan’s thrillers, The Thirty-Nine Steps has been continuously in print since first publication and has been filmed three times, most notably by Alfred Hitchcock in 1935. In this, the only critical edition, Christopher Harvie’s introduction interweaves the writing of the tale with the equally fascinating story of how John Buchan, publisher and lawyer, came in from the cold and, via The Thirty-Nine Steps, ended the war as spy-master and propaganda chief.
My Ántonia
Authors: Cather Willa
Published: June 2023

My Antonia (1918) depicts the pioneering period of European settlement on the tall-grass prairie of the American midwest, with its beautiful yet terrifying landscape, rich ethnic mix of immigrants and native-born Americans, and communities who share life’s joys and sorrows. Jim Burden recounts his memories of Antonia Shimerda, whose family settle in Nebraska from Bohemia. Together they share childhoods spent in a new world. Jim leaves the prairie for college and a career in the east, while Antonia devotes herself to her large family and productive farm. Her story is that of the land itself, a moving portrait of endurance and strength.

Described on publication as ‘one of the best [novels] that any American has ever done’, My Antonia paradoxically took Cather out of the rank of provincial novelists as the same time that it celebrated the provinces, and mythologized a period of American history that had to be lost before its value could be understood.
XVI
Authors: Wharton Edith
Published: June 2023

When Archer walked down the sandy main street of St Augustine to the house which had been pointed out to him as Mr Welland’s, and saw May Welland standing under a magnolia with the sun in her hair, he wondered why he had waited...
XX
Authors: Wharton Edith
Published: June 2023

Of course we must dine with Mrs Carfry, dearest,’ Archer said; and his wife looked at him with an anxious frown across the monumental Britannia ware* of their lodging house breakfast-table.

In all the rainy desert of autumnal London there were only two ...
XXII
Authors: Wharton Edith
Published: June 2023

‘A Party for the Blenkers—the Blenkers?’

Mr Welland laid down his knife and fork and looked anxiously and incredulously across the luncheon-table at his wife, who, adjusting her gold eye-glasses, read aloud, in the tone of high comedy: ‘Professor and Mrs Emerson Sillerton request...
XXIV
Authors: Wharton Edith
Published: June 2023

They lunched slowly and meditatively, with mute intervals between rushes of talk; for, the spell once broken, they had much to say, and yet moments when saying became the mere accompaniment to long duologues of silence. Archer kept the talk from his own affairs,...
XXVII
Authors: Wharton Edith
Published: June 2023

Wall Street, the next day, had more reassuring reports of Beaufort’s situation. They were not definite, but they were hopeful. It was generally understood that he could call on powerful influences in case of emergency, and that he had done so with success; and...
The Leading Conceptions of Elementary Religion
Authors: Durkheim Émile
Published: June 2023

Animism

Supplied with this definition, we can begin our search for the elementary religion we intend to find.

Even the crudest religions made familiar to us by history and ethnography have a complexity that belies the common notion of the primitive mentality. They display not...
The Notion of Soul
Authors: Durkheim Émile
Published: June 2023

In the previous chapters we studied the basic principles of totemic religion. We could see that it contains no idea of soul, spirit, or mythic personality. Yet although the notion of spiritual beings is not fundamental to totemism, and so to religious thought in...
The Negative Cult and its Functions
Authors: Durkheim Émile
Published: June 2023

Ascetic Rites

In what follows, we do not intend to offer a complete description of the primitive cult. Since our main concern is to discover what is most elementary and basic in religious life, we will not try to reconstitute in detail the often...
How Dorothy saved the Scarecrow.
Authors: Baum L. Frank , Denslow W. W.
Published: June 2023

When Dorothy was left alone she began to feel hungry. So she went to the cupboard and cut herself some bread, which she spread with butter. She gave some to Toto, and taking a pail from the shelf she carried it down to...
The Magic Art of the Great Humbug.
Authors: Baum L. Frank , Denslow W. W.
Published: June 2023

Next morning the Scarecrow said to his friends:

“Congratulate me. I am going to Oz to get my brains at last. When I return I shall be as other men are.”

“I have always liked you as you were,” said Dorothy, simply.

“It...
The Dainty China Country.
Authors: Baum L. Frank , Denslow W. W.
Published: June 2023

While the Woodman was making a ladder from wood which he found in the forest Dorothy lay down and slept, for she was tired by the long walk. The Lion also curled himself up to sleep and Toto lay beside him.

The Scarecrow...
Home Again.
Authors: Baum L. Frank , Denslow W. W.
Published: June 2023

A UNT EM had just come out of the house to water the cabbages when she looked up and saw Dorothy running toward her.

“My darling child!” she cried, folding the little girl in her arms and covering her face with kisses; “where...
Clearing the Air
Authors: Childers Erskine
Published: June 2023

‘Has he gone to get the police, do you think?’ said Davies, grimly.

‘I don’t think so,’ said I. ‘Let’s go aboard before that customs fellow buttonholes us.’

A diminished row of stolid Frisians still ruminated over the Dulcibella. Friend Grimm was visible...
Blindfold to Memmert
Authors: Childers Erskine
Published: June 2023

‘Here she comes,’ said Davies. It was nine o’clock on the next day, October 22nd, and we were on deck waiting for the arrival of the steamer from Norddeich. There was no change in the weather—still the same stringent cold, with a high barometer, and...
The Quartette
Authors: Childers Erskine
Published: June 2023

His tour de force was achieved, and for the moment something like collapse set in.

‘What in the world have we come here for?’ he muttered; ‘I feel a bit giddy.’

I made him drink some whisky, which revived him; and then, speaking in whispers,...
Finesse
Authors: Childers Erskine
Published: June 2023

The door of a room on the ground floor was opened to us by a manservant. As we entered the rattle of a piano stopped, and a hot wave of mingled scent and cigar smoke struck my nostrils. The first thing I noticed over Davies’s...
The Governess
Authors: Conrad Joseph
Published: June 2023

“And the best of it was that the danger was all over already. There was no danger any more. The supposed nephew’s appearance had a purpose. He had come, full, full to trembling—with the bigness of his news. There must have been rumours...
On the Pavement
Authors: Conrad Joseph
Published: June 2023

“Fyne was not willing to talk; but as I had been already let into the secret, the fair-minded little man recognized that I had some right to information if I insisted on it. And I did insist, after the third game. We were...
Young Powell Sees and Hears
Authors: Conrad Joseph
Published: June 2023

“You remember,” went on Marlow, “how I feared that Mr. Powell’s want of experience would stand in his way of appreciating the unusual. The unusual I had in my mind was something of a very subtle sort: the unusual in marital relations. I...
Anthony and Flora
Authors: Conrad Joseph
Published: June 2023

Marlow emerged out of the shadow of the bookcase to get himself a cigar from a box which stood on a little table by my side. In the full light of the room I saw in his eyes that slightly mocking expression with which...
O Pioneers!
Authors: Cather Willa
Published: June 2023

“Those fields, colored by various grain!”

Mickiewicz*

to the memory of

SARAH ORNE JEWETT*

in whose beautiful and delicate work

there is the perfection

that endures
II
Authors: Cather Willa
Published: June 2023

On one of the ridges of that wintry waste stood the low log house* in which John Bergson was dying. The Bergson homestead was easier to find than many another, because it overlooked Norway Creek, a shallow, muddy stream that sometimes flowed,...
VI
Authors: Cather Willa
Published: June 2023

At dinner that day Alexandra said she thought they must really manage to go over to the Shabatas’ that afternoon. “It’s not often I let three days go by without seeing Marie. She will think I have forsaken her, now that my old friend...
IX
Authors: Cather Willa
Published: June 2023

On Sunday afternoon, a month after Carl Linstrum’s arrival, he rode with Emil up into the French country to attend a Catholic fair. He sat for most of the afternoon in the basement of the church, where the fair was held, talking to Marie...
XII
Authors: Cather Willa
Published: June 2023

Carl came into the sitting-room while Alexandra was lighting the lamp. She looked up at him as she adjusted the shade. His sharp shoulders stooped as if he were very tired, his face was pale, and there were bluish shadows under his dark eyes....
I
Authors: Cather Willa
Published: June 2023

Ivar was sitting at a cobbler’s bench in the barn, mending harness by the light of a lantern and repeating to himself the 101st Psalm.* It was only five o’clock of a mid-October day, but a storm had come up in the...
II
Authors: Cather Willa
Published: June 2023

Late in the afternoon of a brilliant October day, Alexandra Bergson, dressed in a black suit and traveling-hat, alighted at the Burlington depot in Lincoln. She drove to the Lindell Hotel, where she had stayed two years ago when she came up for Emil’s...
II
Authors: Conrad Joseph
Published: June 2023

Our daily relations were interrupted at this period for something like a fortnight. I had to absent myself unexpectedly from Geneva. On my return I lost no time in directing my steps up the Boulevard des Philosophes.

Through the open door of the drawing-room I...
III
Authors: Conrad Joseph
Published: June 2023

We remained looking at each other for a time.

“Do you know who he is?”

Miss Haldin, coming forward, put this question to me in English.

I took her offered hand.

“Everybody knows. He is a revolutionary feminist, a great writer, if you like, and—how...
V
Authors: Conrad Joseph
Published: June 2023

With a quick inclination of the head for us both, and an earnest, friendly glance at the young man, Miss Haldin left us covering our heads and looking after her straight, supple figure receding rapidly. Her walk was not that hybrid and uncertain gliding...
I
Authors: Conrad Joseph
Published: June 2023

The water under the bridge ran violent and deep. Its slightly undulating rush seemed capable of scouring out a channel for itself through solid granite while you looked. But had it flowed through Razumov’s breast, it could not have washed away the accumulated bitterness...
III
Authors: Conrad Joseph
Published: June 2023

Razumov, thus left to himself, took the direction of the gate. But on this day of many conversations, he discovered that very probably he could not leave the grounds without having to hold another one.

Stepping in view from beyond the lodge appeared the expected...
IV
Authors: Conrad Joseph
Published: June 2023

In his incertitude of the ground on which he stood Razumov felt perturbed. Turning his head quickly, he saw two men on the opposite side of the road. Seeing themselves noticed by Sophia Antonovna, they crossed over at once, and passed one after another...
I
Authors: Conrad Joseph
Published: June 2023

That I should, at the beginning of this retrospect, mention again that Mr. Razumov’s youth had no one in the world, as literally no one as it can be honestly affirmed of any human being, is but a statement of fact from a man...
III
Authors: Conrad Joseph
Published: June 2023

“You will come in for a moment?” said Natalia Haldin.

I demurred on account of the late hour. “You know mother likes you so much,” she insisted.

“I will just come in to hear how your mother is.”

She said, as if to herself,...
The Struggle for an Education
Authors: Washington Booker T.
Published: June 2023

One day, while at work in the coal-mine, I happened to overhear two miners talking about a great school for coloured people somewhere in Virginia. This was the first time that I had ever heard anything about any kind of school or college that...
Helping Others
Authors: Washington Booker T.
Published: June 2023

At the end of my first year at Hampton I was confronted with another difficulty. Most of the students went home to spend their vacation. I had no money with which to go home, but I had to go somewhere. In those days very...
Making their Beds before they could lie on them
Authors: Washington Booker T.
Published: June 2023

A little later in the history of the school we had a visit from General J. F. B. Marshall, the Treasurer of the Hampton Institute, who had had faith enough to lend us the first two hundred and fifty dollars with which to make...
The Atlanta Exposition Address
Authors: Washington Booker T.
Published: June 2023

The Atlanta Exposition, at which I had been asked to make an address as a representative of the Negro race, as stated in the last chapter, was opened with a short address from Governor Bullock. After other interesting exercises, including an invocation from Bishop...
Europe
Authors: Washington Booker T.
Published: June 2023

In 1893 I was married to Miss Margaret James Murray, a native of Mississippi, and a graduate of Fisk University, in Nashville, Tenn., who had come to Tuskegee as a teacher several years before, and at the time we were married was filling the...
Last Words
Authors: Washington Booker T.
Published: June 2023

Before going to Europe some events came into my life which were great surprises to me. In fact, my whole life has largely been one of surprises. I believe that any man’s life will be filled with constant, unexpected encouragements of this kind if...
Why I Write Such Good Books
Authors: Nietzsche Friedrich
Published: June 2023

1

I am one thing, my writings are another.—At this point, before I speak of the writings themselves, let me touch on the question of their being understood or not understood. I’ll do so as casually as is somehow appropriate: for this question...
Why I Am A Destiny
Authors: Nietzsche Friedrich
Published: June 2023

1

I know my lot. Some day my name will be linked to the memory of something monstrous, of a crisis as yet unprecedented on earth, the most profound collision of consciences, a decision conjured up against everything hitherto believed, demanded, hallowed. I...
Anna Victrix
Authors: Lawrence D.H.
Published: June 2023

Will Brangwen had some weeks of holiday after his marriage, so the two took their honeymoon in full hands, alone in their cottage together.

And to him, as the days went by, it was as if the heavens had fallen, and he were sitting with...
Occasional, Critical, and Political Writing
Authors: Joyce James
Published: June 2023

‘I may not be the Jesus Christ I once fondly imagined myself, but I think I must have a talent for journalism’

James Joyce’s non-fictional writings address diverse issues: aesthetics, the functions of the press, censorship, Irish cultural history, England’s literature and empire. This collection includes newspaper articles, reviews, lectures, and propagandizing essays that are consciously public, direct, and communicative. It covers forty years of Joyce’s life and maps important changes in his opinions about politics, especially Irish politics, about the relationship of literature to history, and about writers who remained important to him such as Mangan, Blake, Defoe, Ibsen, Wilde, and Shaw.

These pieces also clarify and illuminate the transformations in Joyce’s fiction, from Dubliners and A Portrait of the Artist as a Young Man to the first drafts of Ulysses. Gathering together more than fifty essays, several of which have never been available in an English edition, this volume is the most complete and the most helpfully annotated collection.
VI
Authors: Conrad Joseph
Published: June 2023

From that evening dated those mysterious but significant phenomena in Schomberg’s establishment which attracted Captain Davidson’s casual notice when he dropped in, placid yet astute, in order to return Mrs. Schomberg’s Indian shawl. And, strangely enough, they lasted some considerable time. It argued either...
The Good Soldier
Authors: Ford Ford Madox
Published: June 2023

Wealthy American John Dowell describes in a disarmingly casual, compellingly intimate manner how he and his wife Florence meet an English couple in a German spa resort. They become friends over the years and gradually the history of their relationships and the passions that lie behind the orderly Edwardian façade are unveiled. Dowell is the archetypal ‘unreliable narrator’, and his casual revelations are both unexpected and explosive.

A masterpiece of early Modernism and a virtuoso performance of literary skill, Ford’s ‘Tale of Passion’ reflects contemporary interests in psychology, sexuality, and the New Woman. Its portrayal of the destruction of a civilized elite anticipates the cataclysm of the First World War, which erupted while Ford was finishing the book. This new edition includes Ford’s important essay ‘On Impressionism’, which sheds valuable light on his artistic technique.
Chapter VI
Authors: Woolf Virginia
Published: June 2023

Orlando went indoors. It was completely still. It was very silent. There was the ink pot: there was the pen; there was the manuscript of her poem, broken off in the middle of a tribute to eternity. She had been about to say, when...
Cell-specific RNA profiling reveals host genes expressed in Arabidopsis cells haustoriated by downy mildew
Authors: Asai Shuta , Cevik Volkan , Jones Jonathan D G , Shirasu Ken
Published: June 2023

The downy mildew oomycete Hyaloperonospora arabidopsidis, an obligate filamentous pathogen, infects Arabidopsis (Arabidopsis thaliana) by forming structures called haustoria inside host cells. Previous transcriptome analyses have revealed that host genes are specifically induced during infection; however, RNA profiling from whole-infected tissues may fail to capture key transcriptional events occurring exclusively in haustoriated host cells, where the pathogen injects virulence effectors to modulate host immunity. To determine interactions between Arabidopsis and H. arabidopsidis at the cellular level, we devised a translating ribosome affinity purification system using 2 high-affinity binding proteins, colicin E9 and Im9 (immunity protein of colicin E9), applicable to pathogen-responsive promoters, thus enabling haustoriated cell-specific RNA profiling. Among the host genes specifically expressed in H. arabidopsidis–haustoriated cells, we found genes that promote either susceptibility or resistance to the pathogen, providing insights into the Arabidopsis–downy mildew interaction. We propose that our protocol for profiling cell-specific transcripts will apply to several stimulus-specific contexts and other plant–pathogen interactions.
Whole-genome assembly of A02 bacteria involved in nitrogen fixation within cassava leaves
Authors: Huang Danping , Ren Jie , Chen Xi , Akhtar Kashif , Liang Qiongyue , Ye Congyu , Xiong Caiyi , He Huahong , He Bing
Published: June 2023

The endophytic nitrogen (N)-fixing bacterium A02 belongs to the genus Curtobacterium (Curtobacterium sp.) and is crucial for the N metabolism of cassava ( Manihot esculenta Crantz). We isolated the A02 strain from cassava cultivar SC205 and used the 15N isotope dilution method to study the impacts of A02 on growth and accumulation of N in cassava seedlings. Furthermore, the whole genome was sequenced to determine the N-fixation mechanism of A02. Compared with low N control (T1), inoculation with the A02 strain (T2) showed the highest increase in leaf and root dry weight of cassava seedlings, and 120.3 nmol/(mL·h) was the highest nitrogenase activity recorded in leaves, which were considered the main site for colonization and N-fixation. The genome of A02 was 3,555,568 bp in size and contained a circular chromosome and a plasmid. Comparison with the genomes of other short bacilli revealed that strain A02 showed evolutionary proximity to the endophytic bacterium NS330 (Curtobacterium citreum) isolated from rice (Oryza sativa) in India. The genome of A02 contained 13 nitrogen fixation (nif) genes, including 4 nifB, 1 nifR3, 2 nifH, 1 nifU, 1 nifD, 1 nifK, 1 nifE, 1 nifN, and 1 nifC, and formed a relatively complete N fixation gene cluster 8-kb long that accounted for 0.22% of the whole genome length. The nifHDK of strain A02 (Curtobacterium sp.) is identical to the Frankia alignment. Function prediction showed high copy number of the nifB gene was related to the oxygen protection mechanism. Our findings provide exciting information about the bacterial genome in relation to N support for transcriptomic and functional studies for increasing N use efficiency in cassava.
Monitoring nutrients in plants with genetically encoded sensors: achievements and perspectives
Authors: Sadoine Mayuri , De Michele Roberto , Župunski Milan , Grossmann Guido , Castro-Rodríguez Vanessa
Published: June 2023

Understanding mechanisms of nutrient allocation in organisms requires precise knowledge of the spatiotemporal dynamics of small molecules in vivo. Genetically encoded sensors are powerful tools for studying nutrient distribution and dynamics, as they enable minimally invasive monitoring of nutrient steady-state levels in situ. Numerous types of genetically encoded sensors for nutrients have been designed and applied in mammalian cells and fungi. However, to date, their application for visualizing changing nutrient levels in planta remains limited. Systematic sensor-based approaches could provide the quantitative, kinetic information on tissue-specific, cellular, and subcellular distributions and dynamics of nutrients in situ that is needed for the development of theoretical nutrient flux models that form the basis for future crop engineering. Here, we review various approaches that can be used to measure nutrients in planta with an overview over conventional techniques, as well as genetically encoded sensors currently available for nutrient monitoring, and discuss their strengths and limitations. We provide a list of currently available sensors and summarize approaches for their application at the level of cellular compartments and organelles. When used in combination with bioassays on intact organisms and precise, yet destructive analytical methods, the spatiotemporal resolution of sensors offers the prospect of a holistic understanding of nutrient flux in plants.
Understanding ‘flexing’: the impact on mental health and public trust
Authors: Rachman Ali , Verawati Inuriya , Rusandi M Arli
Published: June 2023

Background

This correspondence explores the rising phenomenon of ‘flexing’ on social media, characterized by flaunting wealth and luxurious lifestyles. This trend is particularly prominent among influencers and some public officials in Indonesia.

Problem

We identify ‘flexing’ as a behavior that potentially harms both mental health and societal trust, creating an environment that contrasts starkly with the beneficial practice of ‘sharenting,’ which promotes sharing parental experiences for mutual support and healing.

Implications

The influence of ‘flexing’ on public mental health and trust in the tax system requires thorough examination.

Conclusion

Given its adverse effects, the correspondence emphasizes the need for comprehensive measures to address this issue.
Managing patients with sex-, race-, or ethnicity-based cardiovascular health inequities
Authors: Luntz Allison , Creary Kashif , Bruessow Diane
Published: June 2023

Cardiovascular health inequities are experienced among cisgender women, gender minorities, Black and Indigenous people, and people with lower socioeconomic status. Early identification and treatment of patients at risk for disparate and adverse cardiac health outcomes are essential.
Expression of angiogenic and lymphangiogenic genes in primary cutaneous melanoma: relationship with angiolymphatic invasion and disease-free survival
Authors: García-Pérez Omar , Melgar-Vilaplana Leticia , Sifaoui Ines , García-Bello Miguel-Ángel , Córdoba-Lanús Elizabeth , Fernández-de-Misa Ricardo
Published: June 2023

Melanoma is one of the most common cancers in the world. The main routes of tumor progression are related to angiogenesis and lymphangiogenesis. These routes can occur by local invasion, which is called angiolymphatic invasion (ALI). In this study, we assess gene expression of relevant biomarkers of angiogenesis and lymphangiogenesis in 80 FFPE melanoma samples to determine a molecular profile that correlates with ALI, tumor progression, and disease-free survival. The results were enhanced by a posttranscriptional analysis by an immunofluorescence assay. Three SNPs in the VEGFR-2 gene were genotyped in 237 malignant melanoma (MM) blood DNA samples by qPCR.

A significant correlation was found for LYVE-1 and ALI, qualitative (P = 0.017) and quantitative (P = 0.005). An increased expression of protein LIVE-1 in ALI samples supported these results (P = 0.032).

VEGFR2 was lower in patients who showed disease progression (P = 0.005) and protein VEGFR2 posttranscriptional expression decreased (P = 0.016). DFS curves showed differences (P = 0.023) for VEGFR2 expression detected versus the absence of VEGFR2 expression.

No significant influence on DFS was detected for the remaining analyzed genes. Cox regression analysis suggested that VEGFR2 expression has a protective role (HR = 0.728; 95% CI = 0.552–0.962; P = 0.025) on disease progression. No significant association was found between any of the studied SNPs of VEGFR2 and DFS or progression rate.

Our main results suggest that LYVE-1 gene expression is closely related to ALI; the relationship with the development of metastases in MM deserves further studies. Low expression of VEGFR2 was associated with disease progression and the expression of VEGFR2 correlates with an increased DFS.