bluuebunny/crossref_metadata_2025 huggingface dataset:
Universal Silicon Ring Resonator for Error-Free Transmission Links
Authors: Zhang Weiwei , Zhu Junbo , Li Ke , Pant Bharat , Ebert Martin , Yan Xingzhao , Banakar Mehdi , Tran Dehn T. , Littlejohns Callum G. , Gan Fuwan , Reed Graham , Thomson David
Published: September 2023

We report the design, fabrication, and characterization of a universal silicon p-n junction ring resonator for C band error-free communication links operated up to 50 Gb/s with co-designed optical modulation and detection performance. The universal p-n junction ring device shows co-designed detection responsivity up to 0.84 A/W, and in conjunction with a modulation efficiency of ~4 Vmm and >8 dB optical modulation extinction ratio (ER), enabling C band 50-Gb/s NRZ communication link with bit error rate (BER) ≤ 3×10-12. Individually, the speed of modulation and detection is measured up to 112 Gb/s and 80 Gb/s, respectively. The principle of co-designing PN junction ring modulator and detector performance required for error-free communication links can significantly ease the fabrication yield challenges of ring structures by reducing the number of types of devices. The principle can also be applied to O band wavelengths. To the best of our knowledge, for the first time, a device of this type has achieved both error-free modulation and detection operation up to 50 Gb/s in the C band individually or in conjugation as an error free communication link, which paves the way to realize a >1.6 Tb/s all-silicon WDM based error free optical transceiver link in future and is essential for future programmable photonics circuits.
Highly Permeable and Shelf-stable Aquaporin Biomimetic Membrane Based on an Anodic Aluminum Oxide Substrate
Authors: Jeon Tae-Joon , Fuwad Ahmed , Ryu Hyunil , Han Eui Don , Lee Jun-Hee , Malmstadt Noah , Kim Young-Rok , Seo Young Ho , Kim Sun Min
Published: September 2023

Aquaporin (AQP) biomimetic membranes are a coming-of-age technology for water purification. Although several studies have reported aquaporin biomimetic membrane fabrication to date, these membranes show low water flux mainly due to the low porosity and inherently dense structure of the polymeric substrate materials. Herein, we report a ceramic-based aquaporin biomimetic membrane based on anodic aluminum oxide (AAO) as a substrate, which has a uniform porous structure with high aspect ratio and pore density compared to conventional polymer substrates, and exhibits the high water flux of 27.6 ± 3.6 LMH (L/m2h) and superior membrane selectivity of 0.11 g/L. Briefly, the AAO substrate was functionalized with amino-silane followed by polydopamine coating, then the AQP vesicles were immobilized on the functionalized AAO substrate surface using an electrokinetic method, and the water rejection performance of the membrane is analyzed in a forward osmosis system. Furthermore, a simple cryodesiccation method is introduced to improve the storage stability and easy transportation of aquaporin membranes, which does not require special environmental conditions to transport or store them.
Long-term severe hypoxia adaptation induces non-canonical EMT and a novel Wilms Tumor 1 (WT1) isoform.
Authors: Gagnon Etienne , Quenneville Jordan , Feghaly Albert , Tual Margaux , Major François
Published: September 2023

The majority of cancer deaths are caused by solid tumors, where the four most prevalent cancers (breast, lung, colorectal and prostate) account for more than 60% of all cases (1). Tumor cell het-erogeneity driven by variable cancer microenvironments, such as hypoxia, is a key determinant of therapeutic outcome. We developed a novel culture protocol, termed the Long-Term Hypoxia (LTHY) time course, to recapitulate the gradual development of severe hypoxia seen in vivo, to mimic conditions observed in primary tumors. Cells subjected to LTHY underwent a non-canonical epithelial to mesenchymal transition (EMT) based on miRNA and mRNA signatures as well as displayed EMT-like morphological changes. Concomitant to this, we report production of a novel truncated isoform of WT1 transcription factor (tWt1), a non-canonical EMT driver, with expres-sion driven by a yet undescribed intronic promoter through hypoxia-responsive elements (HREs). We further demonstrated that tWt1 initiates translation from an intron-derived start codon, retains proper subcellular localization, DNA binding, and its human ortholog negatively predicts long-term patient survival. Our study demonstrates the importance of culture conditions that better mimic those observed in cancers, especially with regards to hypoxia, and identifies a novel isoform of WT1 which correlates with poor long-term survival.
Ameliorative Effects of Mesenchymal Stem Cells on Senescence Associated Phenotypes in Naturally Aged Rats
Authors: Wang Lu , Deng Zihui , Li Yun , Wu Yiqi , Yao Renqi , Cao Yuan , Wang Min , Zhou Feihu , Zhu Hanyu , Kang Hong-Jun
Published: September 2023

Background
Aging is a multifaceted process that affects all organ systems. With the increasing trend of population aging, age-related diseases have resulted in significant medical challenges and socioeconomic burdens. Mesenchymal stem cells (MSCs), due to their antioxidative stress, immunoregulatory, and tissue repair capabilities, hold promise as a potential anti-aging intervention.
Methods
In this study, we transplanted MSCs into naturally aged rats at 24 months, and subsequently examined levels of aging-related factors such as β-galactosidase, superoxide dismutase, and malondialdehyde in multiple organs. Additionally, we assessed various aging-related phenotypes in these aged rats, including immune senescence, lipid deposition, myocardial fibrosis, and tissue damage. We also conducted a 16S rRNA analysis to study the composition of gut microbiota.
Results
The results indicated that MSCs significantly reduced the levels of aging-associated and oxidative stress-related factors in multiple organs such as the heart, liver, and lungs of naturally aging rats. Furthermore, they mitigated chronic tissue damage and inflammation caused by aging, reduced levels of liver lipid deposition and myocardial fibrosis, alleviated aging-associated immunodeficiency and immune cell apoptosis, and positively influenced the gut microbiota composition towards a more youthful state. This research underscores the diverse anti-aging effects of MSCs, including oxidative stress reduction, tissue repair, metabolic regulation, and improvement of immune functions, shedding light on the underlying anti-aging mechanisms associated with MSCs.
Conclusions
The study confirms that MSCs hold great promise as a potential anti-aging approach, offering the possibility of extending lifespan and improving the quality of life in the elderly population.
In the era of Bortezomib-based Induction, intensification of Melphalan-based conditioning with Bortezomib does not improve Survival Outcomes in newly diagnosed Multiple Myeloma: a study from the Chronic Malignancies Working Party of the EBMT
Authors: Beksac Meral , Eikema Dirk-Jan , Koster Linda , Hulin Cyrille , Poiré Xavier , Hamladji Rose-Marie , Gromek Tomaz , Bazarbachi Ali , Ozkurt Zubeyde , Pabst Thomas , Othman Tarek Ben , Finke Jürgen , Pirogova Olga , Wu Depei , Hayat Amjad , Hilgendorf Inken , Tholouli Eleni , de Wreede Liesbeth , Schönland Stefan , Garderet Laurent , Drozd-Sokolowska Joanna , Raj Kavita , Hayden Patrick , Yakoub-Agha Ibrahim , McLornan Donal
Published: September 2023

Bortezomib (Vel)- Melphalan 200mg/m2 (Mel200) (Vel-Mel) has been utilised to intensify conditioning in autologous hematopoietic stem cell transplantation (AHCT) for multiple myeloma (MM). This EBMT registry-based study compared Vel-Mel with Mel200 during upfront AHCT. Between 2010 and 2017, MM patients who received Vel-Mel (n = 292) conditioning were compared with 4,096 Mel200 patients in the same 58 centres. Pre-AHCT, compared to Mel200 patients, Vel-Mel patients had similar International Staging System (ISS) scores and cytogenetic risk profiles; a similar proportion had received bortezomib-based induction (85% and 87.3%, respectively) though they were younger with a better performance status. Vel-Mel patients were more likely to achieve CR post-induction (40.6% vs 20.3%, p < 0.001) and by day 100 of AHCT (CR/VGPR: 70.2% vs. 57.2%, p < 0.001). There was no difference in 3-year PFS (49% vs 46%, p = 0.06) or early post-AHCT mortality. In multivariable analysis, Vel-Mel associated with inferior PFS (HR: 1.69 (1.27–2.25, p < 0.001) and OS (HR:1.46 (1.14–1.86,p = 0.002), similar to negative effects on PFS of advanced ISS (HR:1.56 (1.33–1.83, p < 0.001), high-risk cytogenetics (HR:1.43(1.18–1.74, p < 0.001) and poor post-induction response( < = PR)(HR: 1.43(1.25–1.62, p < 0.001) Overall, despite superior pre- and post-AHCT responses, there was no improvement in PFS or OS following Vel-Mel. This data supports the findings of the smaller prospective IFM study.
Annexin-A5 is fundamental for VDAC1-dependent mitochondrial Ca2+ homeostasis and determines the susceptibility to apoptosis.
Authors: Graier Wolfgang , Oflaz Furkan , Bondarenko Alexander , Trenker Michael , Waldeck-Weiermair Markus , Gottschalk Benjamin , Bernhart Eva , Koshenov Zhanat , Radulović Snježana , Rost Rene , Hirtl Martin , Pilic Johannes , Leitinger Gerd , Brachvogel Bent , Summerauer Susanne , Shoshan-Barmatz Varda , Malli Roland
Published: September 2023

Annexin-A5 (AnxA5) is a Ca2+-dependent phospholipid-binding protein associated with the regulation of intracellular Ca2+ homeostasis. However, the precise role of AnxA5 in controlling mitochondrial Ca2+ signaling remained elusive. Here, we introduce a novel function of AnxA5 in regulating mitochondrial Ca2+ signaling. Our investigation revealed that AnxA5 orchestrates intermembrane space (IMS) Ca2+ access capacity upon high Ca2+ elevations induced by ER Ca2+ release, leading to AnxA5 translocation to the outer mitochondrial membrane. Through close association with the voltage-dependent anion channel (VDAC1), AnxA5 regulates the Ca2+-permeable state of VDAC1. By modulating IMS Ca2+ signaling, AnxA5 actively shapes mitochondrial ultrastructure and influences the dynamicity of the mitochondrial Ca2+ uniporter. Furthermore, our findings regarding AnxA5's localization in the VDAC1 microenvironment reveal its protective role in regulating cell death by controlling VDAC1's oligomeric state triggered by cisplatin-induced apoptosis. Our study uncovers AnxA5 as a regulator of VDAC1 in physiological and pathological conditions.
Composition of the rumen archaeal community of growing camels fed different concentrates levels
Authors: Rabee Alaa Emara , Askar Ahmed R.
Published: September 2023

Understanding the rumen fermentation and methanogenic community in camels fed intensively is critical for optimizing rumen fermentation, improving feed efficiency, and lowering methane emissions. Using Illumina MiSeq sequencing, quantitative real-time PCR, and high-performance liquid chromatography, this study evaluates the influence of different concentrate supplement levels in the diet on rumen fermentation as well as the diversity and structure of the rumen methanogenic community for growing dromedary camels. Twelve growing camels, 275 ± 8.75 kg body weight, were divided into three groups and given three levels of concentrate supplement, 0.7% (C1), 1% (C2), and 1.3% (C3) based on their body weight. All animals were fed alfalfa hay ad libitum. The levels of total volatile fatty acid, rumen ammonia, and methanogen copy number were unaffected by the supplementation level. Increasing the concentrate supplement level increased the proportion of propionic acid while decreasing the proportion of acetic acid. Increasing the level of concentrate in the diet had no effect on alpha diversity metrics or beta diversity of rumen methanogens. Methanobrevibacter and Methanosphaera predominated the methanogenic community and were declined as concentrate supplement level increased. This study sheds new light on the effect of concentrate supplement level in growing camels' diet on rumen fermentation and methanogenic community, which could help in the development of a strategy that aimed to reduce methane emissions and enhance feed efficiency.
Comparative RNA-Seq Analysis of Differentially Expressed Genes in the Sertoli cells of Yak and Cattle-yak
Authors: Chen Xue-Mei , Wang Ming-Xiu , Zhang Peng , Jing Ke-Min , Yue Bing-Lin , Wu Zhi-Juan , Chai Zhi-Xin , Liu Xin-Rui , Cai Xin
Published: September 2023

Background
To study the problem of male sterility of cattle-yak and improve the yak crossbreeding, this study obtained the testicular Sertoli cells of yak and cattle-yak and compared the differences in transcriptome levels between the two bovine species. The testicular tissues of 3 healthy male cattle-yaks and 3 F1 generation male yaks were collected at the age of 24 months. The Sertoli cells were isolated after enzymatic digestion, differential adhesion and starvation treatment. DATA-4 and SOX9 immunofluorescence staining were used to identify the cell type. Sertoli cells were subjected to transcriptome sequencing, GO analysis, KEGG analysis and differentially expressed gene validation.
Results
The study successfully isolated and purified Sertoli cells of yak and cattle-yak. The transcriptome sequencing data were compared, analyzed and annotated. Compared to yak Sertoli cells, 6592 differentially expressed genes were obtained, 3007 genes were upregulated and 3585 genes were downregulated in cattle-yak Sertoli cells. GO analysis showed that upregulated genes were mainly involved in translation, peptide biosynthetic process, amide biosynthetic process, peptide metabolic process, ribosome, cytoplasmic part, structural constituent of ribosome, structural molecule activity, endomembrane system, protein kinase activity, phosphotransferase activity, etc. The downregulated genes were mainly involved in protein phosphorylation, phosphorylation, endomembrane system, protein kinase activity, phosphotransferase activity, etc. KEGG analysis compared differential genes to 316 pathways, of which 8 pathways were significantly enriched. The upregulated pathways were significantly enriched in cattle-yak Sertoli cells, including ribosome, thermogenesis, oxidative phosphorylation, etc. The downregulated pathways were significantly enriched in adherens junction, mTOR signaling pathway, AMPK signaling pathway, FoxO signaling pathway, focal adhesion, etc.
Conclusions
Compared with yak Sertoli cells, the expression of genes related to protein activation, cell function and membranous organelle composition in cattle-yak Sertoli cells was abnormal. The defects of cattle-yak Sertoli cells cannot provide a suitable environment for spermatogenesis which may be one of the reasons for male cattle-yak sterility.
Comparison of AirAngel® vs Storz® videolaryngoscope and Macintosh® laryngoscope for endotracheal intubation training: Prospective randomized crossover study
Authors: Merter Şeyhmus , Kayayurt Kamil , Kitapçıoğlu Dilek , Yaylaci Serpil
Published: September 2023

Background: Video laryngoscopy (VL) outperforms direct laryngoscopy (DL) in both normal and difficult airway situations. However, VL seems far from being offered as a standard option in both healthcare delivery and educational activities in low-income countries, considering its high costs. Therefore, three dimensional (3D)-printed VLs may be considered an alternative to conventional VLs in low-income countries and other places with limited resources. Our objective was to compare the efficacy of AirAngel 3D-printed VL (3D-PVL) with those of commercially available Storz® VL (SVL) and conventional Macintosh® laryngoscope (MCL) in normal and difficult airway scenarios in the hands of inexperienced users.
Methods: This is a prospective randomized crossover manikin study that included 126 senior medical students with no experience in intubation. The effectiveness of all three laryngoscopy devices in the hands of inexperienced users was evaluated in terms of intubation time, glottic visualization, ease of use, endotracheal tube placement and intubation success rate. Between 2020 and 2022, 126 last year medical students participated in the study.
Results: MCL resulted in significantly longer intubation times than 3D-PVL and SVL in the difficult airway scenario, with no significant difference between 3DPVL and SVL (Wilcoxon test, p < 0.016; Bonferroni correction MCL: 28.54 s; SVL: 26.68 s; 3DPVL: 26.64 s). Both SVL and 3D-PVL resulted in significantly better Cormack−Lehane grades in both normal and difficult airway scenarios, and thus provided better glottic viewing than MCL, with no significant difference between 3D-PVL and SVL (Wilcoxon test, p < 0.016; Bonferroni correction, MCL: 1.73; SVL: 1.29; 3DPVL: 1.25). SVL was the easiest (1: very easy, 5: very difficult) device for normal airway scenarios, while MCL was the most difficult (MCL: 2.64; 3DPVL: 1.98; SVL: 1.49). Conversely, no significant difference was found between 3DPVL and other devices in terms of ease of use in difficult airway scenarios and in terms of accurate placement of the endotracheal tube and successful intubation attempts.
Conclusion: 3D-PVL is a good educational and possible clinical alternative to conventional VL, particularly in places with limited resources, due to its low cost.
Development of a novel cancer survivorship database to describe health care utilization patterns for Coloradans who have completed primary cancer treatment
Authors: Callaway Carlin , Molina Elizabeth , Overholser Linda , Das Santi
Published: September 2023

Purpose Electronic health records (EHR) and data warehouses hold promise for population health management. Specific aims were: (a) to build a comprehensive database representing adult Coloradans who completed cancer treatment within a health care system, and (b) to conduct a secondary analysis of this database.Methods A survivorship database (HDC-SD) was built from the Health Data Compass (HDC) warehouse, which includes diagnostic and treatment information and incorporates all-payer data to identify individuals with histories of cancer who received treatment at the University of Colorado Cancer Center (UCCC) between January 1, 2020 and December 31, 2021. Data was analyzed using Chi-square tests to compare sociodemographic characteristics, disease characteristics, and health maintenance between urban and rural settings.Results The HDC-SD includes 1933 records representing 13 categories of cancers. The majority live in an urban setting (89.8%). Patients in the database living in urban areas had statistically (higher/lower) rates of completing colorectal screening, mammography, flu shots, and COVID-19 vaccination. Emergency department visits occurred at a statistically significant (higher/lower) level for those living in urban areas.Conclusions Creating a database of individuals who have completed active cancer treatment, while incorporating longitudinal health utilization data, may help prepare for systematic population management for cancer survivors.
Effects of pain associated with orthodontic tooth movement on tactile sensation of periodontal ligaments
Authors: Shimada Eriya , Kanetaka Hiroyasu , Hihara Hiroki , Kanno Akitake , Kawashima Ryuta , Nakasato Nobukazu , Igarashi Kaoru
Published: September 2023

Objectives Pain associated with orthodontic tooth movement reportedly reduces periodontal ligament tactile sensation. However, the mechanism at the central nervous system remains unclear. This study was designed to clarify the effects of pain associated with orthodontic tooth movement on periodontal ligament tactile sensation by measuring somatosensory evoked magnetic fields (SEFs) during mechanical stimulation of teeth being moved by separator elastics.
MethodsSEFs were measured using magnetoencephalography during the application of mechanical stimulations to the mandibular right first molars of 23 right-handed healthy subjects (0 hr). Separator elastics were then inserted into the mesial and distal interdental portions of the mandibular right first molars. The same mechanical stimulations were applied to them again 24 hr later. Then the SEFs were measured (24 hr). After each SEF measurement, pain was also evaluated using the Visual Analog Scale (VAS).
Results The VAS values were significantly higher at 24 hr than at 0 hr (p<0.05). Although no significant difference in the peak latencies was found between those obtained at 0 hr and 24 hr, the intensities around 40.0 ms in the contralateral hemisphere were significantly lower at 24 hr than at 0 hr (p<0.01).
ConclusionsPain associated with orthodontic tooth movement might suppress periodontal ligament tactile sensation in the primary somatosensory cortex.
Clinical Relevance Pain associated with orthodontic tooth movement might affect periodontal ligament sensation, consequently causing discomfort during occlusion.
Genome-Wide Identification and Drought stress-induced expression analysis of the Hsp90 gene Family in Potato
Authors: Ji yihong , Zhang Ning , Shao Ziying , Suo Meiqin , Wang Lei
Published: September 2023

Heat shock proteins play important roles in regulating plant growth and development and participating in response to abiotic stresses. However, the role ofHsp90gene family in potato (Solanum tuberosum L.) is yet to be known. In the present study, 9StHsp90s were identified in the potato genome. They were unevenly distributed on 7 chromosomes. Phylogenetic analysis divided theStHsp90s into four subfamilies (Class I–V). Real-time quantitative reverse transcriptase–polymerase chain reaction (RT-qPCR) results detected that 9StHsp90s responded differently to drought stresses. Three genes(PG0022159,PG1006147andPG0019647)had the higher relative expression level than other genes in flowers, which were 9, 16 and 17 times than that in roots, respectively, suggesting their involvement in tissue-specific pattern. This study presents a comprehensive overview of the potatoHsp90gene family and lays a foundation for further analysis of theStHsp90functions on plant development and stress response.
The effect of transcranial direct current stimulation (tDCS) on cognitive function recovery in patients with depression following electroconvulsive therapy (ECT): protocol for a randomized controlled trial
Authors: Hu Renqin , Li Junyao , Lu Yulin , Luo Huirong , Zhang Yinlin , Wang Xueqian , Zhang Zheng , Luo Qinghua
Published: September 2023

Background
Electroconvulsive therapy (ECT) is a highly effective treatment for depressive disorder. However, the use of ECT is limited by its cognitive side effects (CSEs), and no specific intervention has been developed to address this problem. As transcranial direct current stimulation (tDCS) is a safe and useful tool for improving cognitive function, the main objective of this study was to explore the ability to use tDCS after ECT to ameliorate the cognitive side effects.
Methods
Forty-eight eligible participants will be recruited within two days after completing ECT course and randomly assigned to receive either active or sham stimulation in a blinded, parallel-design trial and continue their usual pharmacotherapy. The tDCS protocol consists of 30-min sessions at 2 mA, 5 times per week for 2 consecutive weeks, applied through 15-cm2 electrodes. An anode will be placed over the left dorsolateral prefrontal cortex (DLPFC), and a cathode will be placed over the right supraorbital cortex. Cognitive function and depressive symptoms will be assessed before the first stimulation (T0), after the 5th stimulation (T1), after the final stimulation (T2), 2 weeks after the final stimulation (T3), and 4 weeks after the final stimulation (T4) using the Cambridge Neuropsychological Test Automated Battery (CANTAB).
Discussion
We describe a novel clinical trial to explore whether the administration of tDCS after completing ECT course can accelerates recovery from the CSEs. We hypothesized that the active group would recover faster from the CSEs and be superior to the sham group. If our hypothesis is supported, the use of tDCS could benefit eligible patients who are reluctant to receive ECT and reduce the risk of self-inflicted or suicide due to delays in treatment. Otherwise, the results may indirectly suggest that cognitive impairment may be associated with ECT-induced prefrontal hypometabolism and hypoperfusion.
Trial registration details
The trial protocol is registered with https://www.chictr.org.cn/ under protocol registration number ChiCTR2300071147 (date of registration: 05.06.2023). Recruitment will start in November 2023.
Climatic Zoning of Yerba Mate and Climate Change Projections: A CMIP6 Approach
Authors: Torsoni Guilherme Botega , APARECIDO LUCAS EDUARDO DE OLIVEIRA , Lorençone Pedro Antonio , Lorençone João Antonio , de Lima Rafael Fausto , Rolim Glauco de Souza
Published: September 2023

Yerba mate (Ilex paraguariensis) has several nutritional and pharmaceutical properties and is traditionally used in South America in the preparation of various types of beverages. The pharmaceutical industry has a large number of patents for products related to the properties of this plant. This study aims to analyze the climatic zoning of the yerba mate relative to climate change using CMIP6 model projections. Understanding the potential effects of climate change on yerba mate production and distribution is essential due to its economic and cultural importance. The CMIP6 model allows the analysis of future scenarios by identifying favorable and unfavorable areas for cultivation. The results provide subsidies for adaptation and mitigation measures, helping in strategic planning and decision-making related to sustainable yerba mate production. This study aimed to identify favorable areas for Ilex paraguariensis cultivation for the main producing countries, that is, Brazil, Argentina, Paraguay, and Uruguay, under the CMIP 6 climate change scenarios. The study was conducted in producing regions of the North, Northeast, Midwest, Southeast, and South of Brazil and the countries Paraguay, Argentina, and Uruguay. The ideal air temperature for crop development ranges from 21 to 25°C and the ideal precipitation is > 1200 mm per cycle. Daily air temperature and precipitation data for the current scenario were collected using the WorldClim version 2 platform. Projections of future climate variables were obtained from the WorldClim 2.1 platform using the IPSL-CM6A-LR model, with a 30-second spatial resolution. Four shared socio-economic pathway scenarios were considered for four different periods: 2021–2040, 2041–2060, 2061–2081, and 2081–2100. Data from a geographic information system were used to carry out spatial interpolation throughout the Brazilian territory, using the Kriging method. The results showed that most of the studied territory is classified as unfavorable for yerba mate cultivation, with only 12.25% (1.5 million km2) considered favorable. The highest concentration of favorable areas is found in the South of Brazil and Uruguay, which are currently the main producers of yerba mate. Most future scenarios of climate change showed a high impact on the climate due to an increase in the mean air temperature and a reduction in precipitation, leading to possible negative impacts on yerba mate cultivation, with a total reduction of favorable areas in producing regions.
Retrospective multicentric comparison of Out-of-specification and Standard-of-care Tisagenlecleucel infusion for Diffuse Large B-cell Lymphoma
Authors: Santoro Armando , De Philippis Chiara , Zucchinetti Cristina , Mannina Daniele , Krampera Mauro , Zinzani Pier Luigi , Chiappella Annalisa , Rocco Alice Di , Orciuolo Enrico , Tisi Maria Chiara , Pistolese Flavio , Giordano Laura , Bramanti Stefania
Published: September 2023

Tisagenlecleucel (Tisa-cel) is an autologous anti-CD19 CAR T-cell that requires a complex manufacturing process. A product with all the quality criteria is acknowledged as “standard-of-care” (SOC), while the ones not fitting the benchmark are considered “out-of-specification” (OOS). This retrospective multicentric study compares the efficacy and safety outcomes of OOS Tisa-cel and SOC products. We enrolled a total of 44 DLBCL patients who underwent the treatment according to Italian Medicines Agency criteria. Among them, 11 received OOS Tisa-cel. After a median follow-up of 19.1 months (range, 0.7–43.9), one-year PFS was 45.5% and 36.4% (p = 0.899) and one-year OS was 47.7% and 55.7% (p = 0.598) for OOS and SOC patients, respectively. CRS occurred in 63.6% of patents in the OOS group vs 81.2% in the SOC group (p = 0.248), grade > 2 CRS in 0% vs 3% (p = 1) and ICANS in 3% vs 9% (p = 0.451), respectively. No patients developed grade > 2 ICANS. No cases of non-relapse mortality have been reported. Our data show that OOS infusion does not increase toxicity and has comparable outcomes to SOC products, suggesting to pursue this option in the absence of therapeutic alternatives. However, larger studies of OOS criteria effect on clinical outcomes are needed.
“Boys Love Boy Toys”: A Qualitative Study Exploring the Role of Fathers in Young Boys’ Gendered Toy Preferences
Authors: Tesolin Julia
Published: September 2023

Early childhood is an especially important period for children’s acquisition of gender related attitudes and beliefs. For boys, fathers serve as one of the primary social influences through which they learn about common gender stereotypes, including gender-typical and cross-gender play. The current qualitative study was conducted with six boys (ages 4–6) and their fathers (ages 34–37), where fathers were first asked to complete an adapted version of the Child-Rearing Sex-Role Attitude Scale, which assessed their attitudes and beliefs about gender related toys, activities, and behaviors. Semi-structured interviews were conducted with the father-son pairs, or with the boys alone, where they participated in a series of toy-preference activities. Descriptive coding and content analysis were used to analyze the responses boys provided for their choices of toys. Responses indicated that boys have a more stereotyped definition of “girl toys” than they do of “boy toys”. Fathers’ self-described beliefs reported on the Sex-Role Attitude Scale were similarly reflected in their behaviours and comments during the interviews with their sons.
Offshore Wind and Wave Energy Can Reduce Total Installed Capacity Required in Zero Emissions Grids
Authors: Gonzalez Natalia , Serna-Torre Paul , Sanchez-Perez Pedro , Davidson Ryan , Murray Bryan , Staadecker Martin , Szinai Julia , Wei Rachel , Kammen Daniel , Sunter Deborah , Hidalgo-Gonzalez Patricia
Published: September 2023

As countries around the world race to decarbonize their power systems in an effort to mitigate climate change, the body of research analyzing paths to zero emissions electricity grids has substantially grown. Although many commercially available technologies are typically included in these studies, only a few of them have considered offshore wind and wave energy as contenders in future zero emissions grids. We model, for the first time, offshore wind and wave energy as independent technologies with the possibility of collocation in a power system capacity expansion model of the Western Interconnection with zero emissions by 2050 and a high geographical and temporal resolution. Our key contributions are to identify cost targets for offshore wind and wave energy to become cost effective, to observe a 17% of reduction in total installed capacity by 2050 when offshore wind and wave energy are fully deployed, and to show how total curtailment decreases as offshore wind increases its deployment.
Evaluation of Ovarian Reserve in Unexplained Infertile Cases : A case controlled study
Authors: Sahin Mustafa , Guven Suleyman , Sal Hidayet , Guven Emine Seda Guvendag
Published: September 2023

Background
The aim of this study was to evaluate the effectiveness of current ovarian reserve tests in unexplained infertile cases.
Material And Methods
This case-control study was conducted on 70 unexplained infertile women were included in Tertiary Iniversity hospital. Both groups of basal FSH, estradiol, Antimullerian hormone (AMH), inhibin B, ovarian volume, total antral follicle count (AFC), ovarian volume, ovarain stromal blood flow (Peak systolic velocity (PSV), S/D (systole and diastole ratio), Resistance index (RI) and pulsatility index (PI)) values were compared.
Results
The mean AMH, inhibin B, PSV and stromal blood flow values of the control group patients was higher than those in unexplained infertility group. However, the values of means of RI and PI of the cases in the control were lower than those in infertility group. When PI's value was ≥ 2.00, its sensitivity was 65.7%, and its specificity was 64.3%. In case of the RI's value was ≥ 0.745, its sensitivity was 65.7%, and its specificity was 62.9%. In order to show ovarian reserve in unexplained infertile cases, the sensitivity values can be sorted from high to low as follows : PI > RI > estradiol > FSH > ovarian volume > AFC > inhibin B > stromal blood flow > PSV > S/D.
Conclusion
In the unexplained infertile patient group with a normal ovarian reserve test results, basal estradiol, decreased PI and RI values may be used as goog ovarian reserve predictors.
Uniform stability for a semilinear laminated Timoshenko beams posed in inhomogeneous medium with localized nonlinear damping
Authors: Mansouri Sabeur
Published: September 2023

This paper is mainly concerned with the stabilization of a semilinear laminated Timoshenko beams, posed in an inhomogeneous medium, under the actionof three nonlinear localized frictional damping terms. Such a problem consists of two identical layers of uniform thickness, taking into account that an adhesive of small thickness is bonding the two surfaces and produces an interfacial slip. The main objective is to prove the uniform decay rates for the energy of the considered problemby imposing minimal amount of support for the damping and with no restrictions around the non-constant coefficients. The proof of the desired result is based on some techniques of the Microlocal Analysis Theory.
Effect of physical activity education on shoulder girdle pain and muscle strength in participants with fibromyalgia: an experimental study
Authors: Couëpel Bastien , Daneau Catherine , Tremblay Mathieu , Javelot Thomas , Abboud Jacques , Pagé Isabelle , Descarreaux Martin
Published: September 2023

Background
In patients with fibromyalgia, exercise and education are recommended to decrease pain level and improve pain management. The latest scientific evidence recommends to focus interventions on the upper limb. The aim of this study was to compare the immediate effect of physical activity education with control group on pain and muscle capacity during repeated upper limb contractions in participants with fibromyalgia.
Method
Fifty-six participants with fibromyalgia were randomized into an experimental group and a control group. The intervention consisted in watching a five-minute video that provided information about fibromyalgia, pain, kinesiophobia and physical activity. The control group watched a neutral five-minute video about beavers in Quebec. Following the video, participants performed a muscular fatigue task consisting in a repeated unilateral shoulder abduction task. At baseline and following the muscular fatigue task, maximal voluntary contraction (MVC) in shoulder abduction was assessed as well as pain level and pressure pain threshold (PPT) in the upper limb. Electromyographic activity was also assess for upper trapezius and middle deltoid muscles. Two-way repeated measures analysis of variance was used to compare the MVC, PPT, and pain level before and after the muscular fatigue task between groups.
Results
The experimental group showed a significantly lower increase in pain than the control group in the middle deltoid muscle (p = 0.002) when assessed by verbal pain rating scale. No significant interaction or main effect of Group and Time were observed for the pain level at the upper trapezius and elbow extensor muscles nor for any of the PPT measures. According to electromyographic data, the median frequency values indicate that neither group experienced muscle fatigue during the repeated contraction task.
Conclusions
The results suggest that a short physical activity education video positively influenced middle deltoid pain following repeated abduction in participants with fibromyalgia. Electromyographic analysis showed no evidence of objective muscle fatigue, suggesting that there might be a partial disconnection between the perception of muscle fatigue and the physiological biomarkers associated with muscle fatigue.
Composition of volatiles of citrus varieties and their effects on the behavior of Diaphorina citri
Authors: Oliveira Leanderson , Mesquita Paulo Roberto Ribeiro , Rodrigues Frederico Medeiros , Barbosa Cristiane Jesus , Fancelli Marilene , Costa João Gomes , Schnadelbach Alessandra Selbach
Published: September 2023

Currently, Huanglongbing (HLB) is the most devastating citrus disease in the world. Until the moment, there is no resistant variety to the disease pathogens and the management of the disease includes the chemical control of the insect vector, the Asian citrus psyllid Diaphorina citri (ACP). The goal of this study was to identify the attractiveness of citrus varieties to ACP using a four-arm olfactometer and to identify the volatile organic compounds emitted by the analyzed plants. The following varieties were used: 'Valencia', 'Westin' and 'Pera' sweet orange scions, also the rootstocks, 'Rangpur lime', 'Sunki Tropical', and 'HTR059' hybrid. The VOCs were extracted using the SPME technique and identified through GC-MS. 'Sunki Tropical', 'Rangpur lime' and 'Valencia' were more attractive to D. citri than the others varieties. The main compounds found in the least attractive varieties were β-pinene and linalol in 'Pera', β-pinene and decanol in 'Westin', β-caryophyllene and γ-elemene in 'HTR 059'. Therefore, 'HTR 059', 'Pera' and 'Westin' are the varieties indicated to implement actions to prevent the introduction and spread of the disease. 'Valencia', 'Sunki Tropical' and 'Rangpur lime' are the least suitable varieties for cultivation when HLB prevention is required.
The dynamics of the El Niño Southern Oscillation diversity in the Recharge Oscillator framework
Authors: Priya Priyamvada , Dommenget Dietmar , McGregor Shayne
Published: September 2023

This study investigates the observed El Niño Southern Oscillation (ENSO) dynamics for the eastern Pacific (EP) and central Pacific (CP) events in reference to the canonical ENSO (T). We use the recharge oscillator (ReOsc) model concept to describe the ENSO phase space, based on the interaction of sea surface temperature (T) and thermocline depth (h), for the different types of ENSO events. We further look at some important statistical characteristics, such as power spectrum and cross-correlation, as essential parameters for understanding the dynamics of ENSO. The results show that the dynamics of the CP and EP events are very different from each other and from the canonical ENSO events. The canonical ENSO (T) events fit closest to the idealised ReOsc model and has the most clearly oscillating ENSO phase space, suggesting it is the most predictable ENSO index. The EP index is similar to the canonical ENSO, but the phase space transitions are less clear, suggesting less of an oscillatory nature and the index is more focussed on extreme El Niño and discharge states. The CP index, in turn, does not have a clear propagation through all phases and are strongly skewed towards the La Niña state. The interaction between CP and h are much weaker, making the mode less predictable. Wind forced shallow water model simulations show that the CP winds do not force significant h tendencies, strongly reducing the delayed negative feedback, which is essential for the ENSO cycle.
Protein profiling in systemic sclerosis patients with different pulmonary complications using proteomic antibody microarray
Authors: Huang Jing , Zhu Honglin , Liu Sijia , Li Mengtao , Li Yisha , Luo Hui , Zuo Xiaoxia
Published: September 2023

Background: Pulmonary arterial hypertension (PAH) and interstitial lung disease (ILD) are leading causes of systemic sclerosis (SSc) -related death. In this study, we aimed to identify biomarkers for detecting SSc pulmonary complications that are mild and in the early stages to improve the prognosis.
Methods: We screened for serum biomarkers using a proteomic antibody microarray that simultaneously assessed 1,000 proteins. Differentially expressed proteins were further verified using ELISA. Finally, we performed a correlation analysis using clinical data.
Results: We identified 125 differentially expressed proteins, of which calcitonin, sclerostin (SOST), CD40, and fibronectin were selected for further verification. Serum calcitonin and SOST levels were significantly elevated in all SSc pulmonary complication subgroups, whereas serum calcitonin levels were higher in the SSc with PAH subgroup than in the SSc without PAH and ILD subgroup. Serum SOST levels were possibly associated with the presence of ILD and positively related to the presence of cardiac and gastrointestinal involvement. Serum CD40 and calcitonin levels appeared to be positively related to the presence of renal involvement, and serum calcitonin was also positively related to the presence of gastrointestinal involvement.
Conclusions: This study indicated that serum calcitonin and SOST levels may be promising biomarkers for SSc-related PAH and ILD, respectively. Further research is needed to verify this result and understand the underlying mechanisms.
Impact of aquaculture practices on the sustainability of social-ecological systems in coastal zones of the Mekong delta
Authors: Hua Hieu Hong , Cremin Emilie , Huynh Van Da , Long Graham , Renaud Fabrice
Published: September 2023

Historically, local communities have thrived in the coastal zones of the Mekong Delta in Vietnam, characterised by diverse habitats such as mangroves, marshes, and mudflats, harbouring significant biodiversity. However, escalating global market demands and price fluctuations have driven the adoption of more intensive aquaculture models, generating new threats to these vulnerable areas. Consequently, coastal regions face compounded hazards (high temperatures, drought, floods, high tides, sea level rise, and saline intrusion), necessitating the identification of socio-economic and ecological drivers of risks to sustainable livelihoods centred around shrimp aquaculture. This research aims to comprehend the impact of various shrimp farming models, ranging from extensive to hyper-intensive, in the context of multiple natural hazards and transformative processes like land conversion and mangrove restoration. By employing risk assessment tools and analysing qualitative and quantitative data gathered from interviews and focus groups with local communities and stakeholders, we present a comprehensive model illustrating the influence of different aquaculture models on coastal socio-ecological systems. Key findings emphasise the interplay between livelihoods, landscape conversion to shrimp farming, and factors guiding such conversions. The paper highlights how these diverse aquaculture models can augment or diminish risks to sustainable livelihoods in the Mekong Delta. It outlines the benefits, constraints, and potential hazards associated with each model and stresses the need for stakeholder collaboration to preserve vital ecosystems and their services. Additionally, the study underlines the importance of improved wastewater treatment practices among farming units to benefit neighbouring farmers and foster overall environmental well-being.
Chitosan functionalized iron oxide nanocomposites as a novel carbonic anhydrase nano-inhibitor
Authors: Mostafa Samar M. , Fathy Mohamed M. , Khalil Wafaa M. , Mohamed Noha
Published: September 2023

This study evaluates the interaction between different molecular weight chitosan functionalized iron oxide nanoparticles (INPs) and carbonic anhydrase II (CAII) as a new approach for the inhibition of CAII. INPs functionalized with different molecular weight chitosan (HC-INPs, MC-INPs, LC-INPs). The characterizations of the synthesized nanocomposites were performed using TEM, XRD, Zeta potential, FTIR, and VSM. The interaction of nanocomposites with CAII was also assessed. The average diameters of prepared nano-formulations were approximate, 4 nm, 11.5 nm, and 20 nm for HC-INPs, MC-INPs, and LC-INPs respectively. Zeta potential values were 39.2 ± 3.9 mV, 36.7 ± 4.1 mV, and 15.5 ± 4.6 mV for HC-INPs, MC-INPs, and LC-INPs respectively. Fluorescence quenching assays and Enzyme Kinetics activity results revealed that the CAII-nanocomposites interaction led to CAII conformational changes that rendered the enzyme inactive. Interestingly, we found that the surface property of nano-inhibitors plays a vital role in their inhibition ability. Whereas HC-INPs have the most potent inhibitory effect with Ki (ng/ml) = 58.388 (ng/ml). While MC-INPs and LC-INPs have Ki=390.87 (ng/ml) and 1213.00 (ng/ml), respectively. The prepared chitosan functionalized iron oxide nanoparticles (especially HC-INPs) are considered a novel nano-inhibitor for CAII which pave the way for using HC-INPs to target disorders related to CAII overexpression.
Thermal evaluation and optimization of a building heating system : radiant floor coupled with a solar system
Authors: Simou Z. , Hamdaoui S. , Oubenmoh S. , Afou Y. El , Babaharra O. , Mahdaoui M. , Msaad A. Ait
Published: September 2023

The continuous increase in the building energy consumption impactes negatively on both energy resources and the environment. In this respect, the use of conventional heating systems affects on both energy consumption and the related co2 emissions. The present work shows the benefits of using a radiant floor heating system (RFHS) coupled with a solar energy system. In the current study TRNSYS-18 software was used to numerically evaluate the RFHS. To achieve improved efficiency and optimize the performance of this letter, a parametric study was conducted on the solar system characteristics and the heated floor. According to the obtained results the optimal design parameters of the system that lead to a high annual solar fraction (SF=93.72%) are: a parabolic trough collector area of 6 m2 with a tilt angle 60°, a mass flow rate of 180 kg/h and 410 kg for the collector and floor heating respectively, and a thickness of 0.07m for the heating floor slab. Furthermore, the total auxiliary energy consumption of the RFHS is approximately 63.42 kWh, versus 1953.41 kWh for a conventional heating system of reference. The economic results of the studied system show that the cost of energy saving is 244.25 €/year, and becomes 257.52 €/year with the production of solar domestic hot water. The payback period is estimated at around 13 years.