bluuebunny/crossref_metadata_2025 huggingface dataset:
Evaluating Perceived Emergency Preparedness and Household Preparedness Behaviors: Results from a CASPER Survey in Fairfax, Virginia
Authors: Ferguson Rennie W. , Kiernan Shawn , Spannhake Ernst W. , Schwartz Benjamin
Published: July 2019

Objectives:

Using data collected from a Community Assessment for Public Health Emergency Response (CASPER) conducted in Fairfax Health District, Virginia, in 2016, we sought to assess the relationship between household-level perceived preparedness and self-reported preparedness behaviors.

Methods:

Weighted population estimates and 95% confidence intervals were reported, and Pearson’s chi-squared test was used to investigate differences by group.

Results:

Examining responses to how prepared respondents felt their household was to handle a large-scale emergency or disaster, an estimated 7.4% of respondents (95% CI: 4.3–12.3) reported that their household was “completely prepared,” 37.3% (95% CI: 31.4–43.7) were “moderately prepared,” 38.2% (95% CI: 31.6–45.2) were “somewhat prepared,” and 14.4% (95% CI: 10.2–20.0) were “unprepared.” A greater proportion of respondents who said that their household was “completely” or “moderately” prepared for an emergency reported engaging in several behaviors related to preparedness. However, for several preparedness behaviors, there were gaps between perceived preparedness and self-reported readiness.

Conclusions:

Community assessments for public health preparedness can provide valuable data about groups who may be at risk during an emergency due to a lack of planning and practice, despite feeling prepared to handle a large-scale emergency or disaster.
Modeling of the 3D spatio-temporal thermal profile of joule-class -based laser amplifiers
Authors: Tamer Issa , Keppler Sebastian , Körner Jörg , Hornung Marco , Hellwing Marco , Schorcht Frank , Hein Joachim , Kaluza Malte C.
Published: July 2019

Thermal profile modification of an active material in a laser amplifier via optical pumping results in a change in the material’s refractive index, and causes thermal expansion and stress, eventually leading to spatial phase aberrations, or even permanent material damage. For this purpose, knowledge of the 3D spatio-temporal thermal profile, which can currently only be retrieved via numerical simulations, is critical for joule-class laser amplifiers to reveal potentially dangerous thermal features within the pumped active materials. In this investigation, a detailed, spatio-temporal numerical simulation was constructed and tested for accuracy against surface thermal measurements of various end-pumped
$\text{Yb}^{3+}$
-doped laser-active materials. The measurements and simulations show an excellent agreement and the model was successfully applied to a joule-class
$\text{Yb}^{3+}$
-based amplifier currently operating in the POLARIS laser system at the Friedrich-Schiller-University and Helmholtz-Institute Jena in Germany.
Circulation and Energy Theorem Preserving Stochastic Fluids
Authors: Drivas Theodore D. , Holm Darryl D.
Published: July 2019

Smooth solutions of the incompressible Euler equations are characterized by the property that circulation around material loops is conserved. This is the Kelvin theorem. Likewise, smooth solutions of Navier–Stokes are characterized by a generalized Kelvin's theorem, introduced by Constantin–Iyer (2008). In this note, we introduce a class of stochastic fluid equations, whose smooth solutions are characterized by natural extensions of the Kelvin theorems of their deterministic counterparts, which hold along certain noisy flows. These equations are called thestochastic Euler–Poincaréandstochastic Navier–Stokes–Poincaréequations respectively. The stochastic Euler–Poincaré equations were previously derived from a stochastic variational principle by Holm (2015), which we briefly review. Solutions of these equations do not obey pathwise energy conservation/dissipation in general. In contrast, we also discuss a class of stochastic fluid models, solutions of which possess energy theorems but do not, in general, preserve circulation theorems.
Food composition of whiting Merlangius merlangus, captured around the sea-cage fish farms in Ordu, south-eastern Black Sea
Authors: Şensurat-Genç Tuğçe , Akyol Okan , Özgül Aytaç , Özden Uğur
Published: July 2019

The food composition of whiting, Merlangius merlangus, caught around the sea-cage fish farms off the coast of Perşembe, was analysed and compared with the diet of whiting from a control site off the coast of Fatsa (Ordu Province, south-eastern Black Sea). A total of 815 stomach samples were collected during the study, and of these, 195 (23.9%) were empty. According to the percentage of relative importance index (IRI%), pellet food (47.8%) and Annelida (25%) were the main prey groups of whiting in the sea-cage fish farms area, while unidentified teleost (85.3%) and Engraulis encrasicolus (8.2%) were dominant in the control site. The other prey groups in both areas were Crustaceans (Mysidae, Amphipoda, Upogebia sp.), Sprattus sprattus and Gobius spp. Seasonally, pellet food was the most consumed food in all seasons, but Mysidae was the first preference of whiting around the sea-cage fish farms in spring. In the control site, unidentified teleost was the first preference in all seasons, except winter, where E. encrasicolus was the first choice, followed by crustaceans and S. sprattus in winter. Bray–Curtis analysis shows that seasonally, there is no significant difference in the sea-cage fish farms, while there is significant difference in the control site, and two-dimensional nMDS of IRI% revealed a clear separation between both sites. SIMPER analysis revealed that the most contributing factors to the differences between seasons were pellet food in the sea-cage fish farms, and E. encrasicolus in the control site.
What is taken for granted in autism research?
Authors: Friedner Michele Ilana
Published: July 2019

This commentary focuses on three points: the need to consider semiotic ideologies of both researchers and autistic people, questions of commensurability, and problems with “the social” as an analytical concept. It ends with a call for new research methodologies that are not deficit-based and that consider a broad range of linguistic and non-linguistic communicative practices.
Beyond autism: Challenging unexamined assumptions about social motivation in typical development
Authors: Bartsch Karen , Estes David
Published: July 2019

In challenging the assumption of autistic social uninterest, Jaswal & Akhtar have opened the door to scrutinizing similar unexamined assumptions embedded in other literatures, such as those on children's typically developing behaviors regarding others’ minds and morals. Extending skeptical analysis to other areas may reveal new approaches for evaluating competing claims regarding social interest in autistic individuals.
Normocentric biases taint cognitive neuroscience and intervention of autism
Authors: Mottron Laurent
Published: July 2019

Stepping away from a normocentric understanding of autism goes beyond questioning the supposed lack of social motivation of autistic people. It evokes subversion of the prevalence of intellectual disability even in non-verbal autism. It also challenges the perceived purposelessness of some restricted interests and repetitive behaviors, and instead interprets them as legitimate exploratory and learning-associated manifestations.
Two sources of bias affecting the evaluation of autistic communication
Authors: Li Pearl Han , Koenig Melissa
Published: July 2019

We support Jaswal & Akhtar's interrogation of social motivational accounts of autism and discuss two sources of bias that contribute to how others construe autistic people's communications: (1) an experience-based bias that limits our ability to discern the speaker's action as communicative and (2) a prejudice against the credibility of certain speakers that limits a listener's willingness to believe their testimony.
Learning how to read autistic behavior from interactions between autistic people
Authors: Heasman Brett , Gillespie Alex
Published: July 2019

Do autistic people read autistic behavior in the same way as neurotypical observers? We consider evidence that suggests autistic-to-autistic interactions demonstrate enabling norms and question the possibilities for neurotypical researchers to learn from autistic social appraisal.
Social motivation in autism: Gaps and directions for measurement of a putative core construct
Authors: Keifer Cara M. , Dichter Gabriel S. , McPartland James C. , Lerner Matthew D.
Published: July 2019

This commentary highlights the observation that social motivation is usually an imprecisely specified construct. We suggest four social motivation conceptualizations across levels of analysis and explore where the target article situates among these. We then offer theoretical and practical guidance for operationalization and measurement of social motivation to support more comprehensive future research on this complex construct in the autism literature.
Socially interested, or socially sophisticated? On mutual social influence in autism
Authors: Forgeot d'Arc Baudouin , Soulières Isabelle
Published: July 2019

A lower tendency to influence and be influenced by their social environment seems almost self-evident in autism. However, a closer look at differences and similarities between autistic and non-autistic individuals suggests that some basic mechanisms involved in social influence might be intact in autism, whereas atypical responses point to differences in more sophisticated recursive social strategies, such as reputation management.
Syntactic development in early foreign language learning: Effects of L1 transfer, input, and individual factors
Authors: Hopp Holger , Steinlen Anja , Schelletter Christina , Piske Thorsten
Published: July 2019

This study explores parallels and differences in the comprehension ofwh-questions and relative clauses between early foreign-language (FL) learners and monolingual children. We test for (a) effects of syntactic first-language (L1) transfer, (b) the impact of input on syntactic development, and (c) the impact of individual differences on early FL syntactic development. We compare the results to findings in child second language (L2) naturalistic acquisition and adult FL acquisition. Following work on adult FL acquisition, we carried out a picture-based interpretation task with 243 child FL learners in fourth grade at different regular, partial, and high-immersion schools in Germany plus 68 monolingual English children aged 5 to 8 years as controls. The child FL learners display a strong subject-first preference but do not appear to use the L1 syntax in comprehension. Input differences across different schools affect overall accuracy, with students at high-immersion FL schools catching up to monolingual performance within 4 years of learning. Finally, phonological awareness is implicated in both early FL learning and naturalistic child L2 development. These findings suggest that early FL development resembles child L2 acquisition in speed and effects of individual factors, yet is different from adult FL acquisition due to the absence of L1 transfer effects.
THE CHANGING SIGNIFICANCE OF LATIN AMERICAN SILVER IN THE CHINESE ECONOMY, 16TH–19THCENTURIES
Authors: Von Glahn Richard
Published: July 2019

The important role of Chinese demand for silver in stimulating worldwide silver-mining and shaping the first truly global trading system has become commonly recognised in the world history scholarship. The commercial dynamism of China during the 16th-19th centuries was integrally related to the importation of foreign silver, initially from Japan but principally from Latin America. Yet the significance of imports of Latin American silver for the Chinese economy changed substantially over these three centuries in tandem with the rhythms of China's domestic economy as well as the global trading system. This article traces these changes, including the adoption of a new standard money of account—the yuan—derived from the Spanish silver peso coin.
Technology and Learner Autonomy: An Argument in Favor of the Nexus of Formal and Informal Language Learning
Authors: Lai Chun
Published: July 2019

This article discusses some of the current research on technology in relation to learner autonomy, outlining major findings on the relationship between technology and learner autonomy in formal and informal learning contexts. Extant literature has discussed both teacher-initiated technology-enhanced formal learning environments and learner-constructed self-directed learning experience in informal learning contexts. Although valuable in the insights it provides into how technology aids learner autonomy, the two bodies of literature have largely been independent from each other, which may constrain our understanding.
Media and Second Dialect Acquisition
Authors: Nycz Jennifer
Published: July 2019

This article addresses the role that different types of media might play in second dialect acquisition. While many scholars agree that broadcast media such as television have little effect on individual speakers’ language use, research across fields (sociolinguistics, second language learning, laboratory phonology, and phonetics) suggests that high levels of engagement could facilitate dialect learning via mediated speech. I will briefly describe the mechanisms underlying acquisition of new dialect features, discuss how these mechanisms might operate when speakers are exposed to speech via specific types of media, and sketch some avenues for future research.
Alignment During Synchronous Video Versus Written Chat L2 Interactions: A Methodological Exploration
Authors: Michel Marije , Cappellini Marco
Published: July 2019

Conversational alignment (i.e., the automatic tendency of interactants to reuse each other's morphosyntactic structures and lexical choices in natural dialogue) is a well-researched phenomenon in native (Pickering & Ferreira, 2008) and to a smaller extent in second language (L2) speakers (Jackson, 2018) as confirmed by many highly controlled lab-based experimental studies investigating face-to-face oral interaction. Only a few studies have explored alignment in more naturally occurring L2 interactions (e.g., Dao, Trofimovich, & Kennedy, 2018), some of them extending the context to written computer-mediated communication (SCMC) (e.g., Michel & Smith, 2018).

The current study aimed to address this gap by taking a closer look at alignment in L2 conversations mediated by two different types of SCMC (videoconference vs. text chat). We explored lexical as well as structural alignment in three target languages (Chinese, French, and German) involving interactional partners of different status (L2 peer, L1 peer, and L1 tutor).

Results revealed that lexical and structural alignment are both present and observable in different SCMC contexts. From a methodological point of view, we discuss how different analyses suit the data generated by the affordances of the different SCMC contexts in the target languages and argue for a more dynamic and pervasive perspective on interaction.
Corpus Linguistics, Learner Corpora, and SLA: Employing Technology to Analyze Language Use
Authors: McEnery Tony , Brezina Vaclav , Gablasova Dana , Banerjee Jayanti
Published: July 2019

In this article we explore the relationship between learner corpus and second language acquisition research. We begin by considering the origins of learner corpus research, noting its roots in smaller scale studies of learner language. This development of learner corpus studies is considered in the broader context of the development of corpus linguistics. We then consider the aspirations that learner corpus researchers have had to engage with second language acquisition research and explore why, to date, the interaction between the two fields has been minimal. By exploring some of the corpus building practices of learner corpus research, and the theoretical goals of second language acquisition studies, we identify reasons for this lack of interaction and make proposals for how this situation could be fruitfully addressed.
Operable ventricular septal defect despite severe pulmonary hypertension and cyanosis!
Authors: Kothari Shyam S. , Relan Jay , Devagourou Velayoudam
Published: July 2019

Patients with a significant left-to-right shunt at ventricular level may become inoperable at an early age due to irreversible pulmonary vascular disease. On the other hand, even suprasystemic pulmonary hypertension due to mitral stenosis remains treatable. We report a 24-year-old patient with large ventricular septal defect, severe mitral stenosis and cyanosis who improved after surgical correction of both the lesions. This emphasises the importance of additional post-capillary pulmonary hypertension in Eisenmenger syndrome.
Englishwhat withabsolute constructions: a Construction Grammar perspective
Authors: KIM JONG-BOK , DAVIES MARK
Published: July 2019

There are several types of absolute constructions (acs) in English. Among these, this article investigates the so-calledwhat-with ac, which has not received much attention in the study of English grammar. This article considers the grammatical properties of the construction from a synchronic as well as a diachronic perspective, using much more representative and robust corpora than previous studies. Based on corpus data drawn from historical corpora such as COHA (Corpus of Historical American English, 400 million words), the article addresses questions about changes in the construction's syntactic, semantic and pragmatic properties. In addition, the article provides a Construction Grammar perspective, which supports previous research in arguing that the construction is undergoing the processes of grammatical constructionalization.
Process Understanding of Soil BVOC Fluxes in Natural Ecosystems: A Review
Authors: Tang J. , Schurgers G. , Rinnan R.
Published: July 2019

Biogenic volatile organic compounds (BVOCs) can be released from soils to the atmosphere through microbial decomposition of plant residues or soil organic carbon, root emission, evaporation of litter‐stored BVOCs, and other physical processes. Soils can also act as a sink of BVOCs through biotic and abiotic uptake. Currently, the source and sink capabilities of soils have not been explicitly accounted for in global BVOC estimates from the terrestrial biosphere. In this review, we summarize the current knowledge of soil BVOC processes and aim to propose a generic framework for modelling soil BVOCs based on current understanding and data availability. To achieve this target, we start by reviewing measured sources and sinks of soil BVOCs and summarize commonly reported compounds. Next, we strive to disentangle the drivers for the underlying biotic and abiotic processes. We have ranked the list of compounds, known to be emitted from soils, based on our current understanding of how each process controls emission and uptake. We then present a modelling framework to describe soil BVOC emissions. The proposed framework is an important step toward initializing modelling exercises related to soil BVOC fluxes. Finally, we also provide suggestions for measurements needed to separate individual processes, as well as explore long‐term and large‐scale patterns in soil BVOC fluxes.
Nonhysteretic Capillary Pressure in Two‐Fluid Porous Medium Systems: Definition, Evaluation, Validation, and Dynamics
Authors: Miller C. T. , Bruning K. , Talbot C. L. , McClure J. E. , Gray W. G.
Published: July 2019

A closure relation for capillary pressure plays an important role in the formulation of both traditional and evolving models of two‐fluid‐phase flow in porous medium systems. We review the traditional approaches to define capillary pressure, to describe it mathematically, to determine parameters for this relation, and to constrain the domain of applicability of this relation. In contrast to the traditional approach, we provide a rigorous, multiscale definition of capillary pressure, define the state domain of interest in practice, summarize computational and experimental approaches to investigate the system state, and apply the methods for two‐fluid states in a model ink bottle system, the classical Finney pack of spheres, and a synthetic sphere pack system. The results of these applications show that a state equation exists that describes capillary pressure without hysteresis. This state equation parameterizes a function that describes the nonwetting phase volume fraction in terms of the capillary pressure, the interfacial area, and the specific Euler characteristic of the nonwetting phase. Furthermore, this state equation applies over the complete range of conditions encountered in practice, and it applies under both equilibrium and dynamic conditions. This state equation involving capillary pressure forms an important foundation for the development of the next generation of macroscale two‐fluid‐phase flow models in porous medium systems.
Initial 40Ar‐39Ar Ages of the Paleocene‐Eocene Boundary Impact Spherules
Authors: Schaller Morgan F. , Turrin Brent D. , Fung Megan K. , Katz Miriam E. , Swisher Carl C.
Published: July 2019

We report 40Ar‐39Ar step‐heating ages of Paleocene‐Eocene (P‐E) boundary impact spherules from Atlantic Margin coastal plain and open ocean sites. We test the hypothesis that the P‐E spherules are reworked from an earlier event (e.g., K‐Pg impact at ~66 Ma), which predicts a cooling age discordant from their depositional age of 55.93 ± 0.05 Ma at the P‐E boundary. Isochrons from the step‐heating analysis yield 40Ar‐36Ar intercepts in excess of the modern in most cases, indicating that the spherules have excess radiogenic Ar (40Ar*), typical of impact glasses incompletely degassed before solidification. The weighted mean of the isochron‐corrected plateau age is 54.2 ± 2.5 Ma (1σ), and their isochron age is 55.4 ± 4.0 Ma, both indistinguishable from their P‐E depositional age, not supporting the K‐Pg reworking hypothesis. This is consistent with all other stratigraphic and geochemical evidence for an impact at the P‐E boundary and ejecta distribution by air fall.
Why Do Antarctic Ozone Recovery Trends Vary?
Authors: Strahan Susan E. , Douglass Anne R. , Damon Megan R.
Published: July 2019

We use satellite ozone records and Global Modeling Initiative chemistry transport model simulations integrated with Modern Era Retrospective for Research and Analysis 2 meteorology to identify a metric that accurately captures the trend in Antarctic ozone attributable to the decline in ozone depleting substances (ODSs). The GMI CTM Baseline simulation with realistically varying ODS levels closely matches observed interannual to decadal scale variations in Antarctic September ozone over the past four decades. The expected increase or recovery trend is obtained from the differences between the Baseline simulation and one with identical meteorology and fixed 1995 ODS levels. The differences show that vortex‐averaged column O3 has the greatest sensitivity to ODS change from 1 to 20 September. The observed vortex‐averaged column O3 during this period produces a trend consistent with the expected recovery attributable to ODS decline. Trends from dates after 20 September have smaller sensitivity to ODS decline and are more uncertain due to transport variability. Simulations show that the greatest decrease in O3 loss (i.e., recovery) occurs inside the vortex near the edge. The polar cap metrics have vortex size‐dependent bias and do not consistently sample this region. Because the 60–90°S 220 Dobson unit O3 mass deficit metric does not sample the edge region, its trend is lower than the expected trend; this is improved by area weighting. The 250‐Dobson unit O3 mass deficit metric samples more of the edge region, which increases its trend. Approximately 25% of the September Antarctic O3 increase is due to higher O3 levels in June prior to winter depletion.
Bayesian Calibration and Sensitivity Analysis for a Karst Aquifer Model Using Active Subspaces
Authors: Teixeira Parente Mario , Bittner Daniel , Mattis Steven A. , Chiogna Gabriele , Wohlmuth Barbara
Published: July 2019

In this article, we perform a parameter study for a recently developed karst hydrological model. The study consists of a high‐dimensional Bayesian inverse problem and a global sensitivity analysis. For the first time in karst hydrology, we use the active subspace method to find directions in the parameter space that dominate the Bayesian update from the prior to the posterior distribution in order to effectively reduce the dimension of the problem and for computational efficiency. Additionally, the calculated active subspace can be exploited to construct sensitivity metrics on each of the individual parameters and be used to construct a natural model surrogate. The model consists of 21 parameters to reproduce the hydrological behavior of spring discharge in a karst aquifer located in the Kerschbaum spring recharge area at Waidhofen a.d. Ybbs in Austria. The experimental spatial and time series data for the inference process were collected by the water works in Waidhofen. We show that this case study has implicit low dimensionality, and we run an adjusted Markov chain Monte Carlo algorithm in a low‐dimensional subspace to construct samples of the posterior distribution. The results are visualized and verified by plots of the posterior's push‐forward distribution displaying the uncertainty in predicting discharge values due to the experimental noise in the data. Finally, a discussion provides hydrological interpretation of these results for the Kerschbaum area.
A brain somatic RHEB doublet mutation causes focal cortical dysplasia type II
Authors: Zhao Shanshan , Li Zhenghui , Zhang Muxian , Zhang Lingliang , Zheng Honghua , Ning Jinhuan , Wang Yanyan , Wang Fengpeng , Zhang Xiaobin , Gan Hexia , Wang Yuanqing , Zhang Xian , Luo Hong , Bu Guojun , Xu Huaxi , Yao Yi , Zhang Yun-wu
Published: July 2019

Focal cortical dysplasia type II (FCDII) is a cerebral cortex malformation characterized by local cortical structure disorganization, neuronal dysmorphology, and refractory epilepsy. Brain somatic mutations in several genes involved in the PI3K/AKT/mTOR pathway are associated with FCDII, but they are only found in a proportion of patients with FCDII. The genetic causes underlying the development FCDII in other patients remain unclear. Here, we carried out whole exome sequencing and targeted sequencing in paired brain–blood DNA from patients with FCDII and identified a brain somatic doublet mutation c.(A104T, C105A) in the Ras homolog, mTORC1 binding (RHEB) gene, which led to the RHEB p.Y35L mutation in one patient with FCDII. This RHEB mutation carrier had a dramatic increase of ribosomal protein S6 phosphorylation, indicating mTOR activation in the region of the brain lesion. The RHEB p.Y35L mutant protein had increased GTPλS-binding activity compared with wild-type RHEB. Overexpression of the RHEB p.Y35L variant in cultured cells also resulted in elevated S6 phosphorylation compared to wild-type RHEB. Importantly, in utero electroporation of the RHEB p.Y35L variant in mice induced S6 phosphorylation, cytomegalic neurons, dysregulated neuron migration, abnormal electroencephalogram, and seizures, all of which are found in patients with FCDII. Rapamycin treatment rescued abnormal electroencephalograms and alleviated seizures in these mice. These results demonstrate that brain somatic mutations in RHEB are also responsible for the pathogenesis of FCDII, indicating that aberrant activation of mTOR signaling is a primary driver and potential drug target for FCDII.
Regulation of zebrafish dorsoventral patterning by phase separation of RNA-binding protein Rbm14
Authors: Xiao Yue , Chen Jiehui , Wan Yihan , Gao Qi , Jing Naihe , Zheng Yixian , Zhu Xueliang
Published: July 2019

RNA-binding proteins with intrinsically disordered regions (IDRs) such as Rbm14 can phase separate in vitro. To what extent the phase separation contributes to their physiological functions is however unclear. Here we show that zebrafish Rbm14 regulates embryonic dorsoventral patterning through phase separation. Zebrafish rbm14 morphants displayed dorsalized phenotypes associated with attenuated BMP signaling. Consistently, depletion of mammalian Rbm14 downregulated BMP regulators and effectors Nanog, Smad4/5, and Id1/2, whereas overexpression of the BMP-related proteins in the morphants significantly restored the developmental defects. Importantly, the IDR of zebrafish Rbm14 demixed into liquid droplets in vitro despite poor sequence conservation with its mammalian counterpart. While its phase separation mutants or IDR failed to rescue the morphants, its chimeric proteins containing an IDR from divergent phase separation proteins were effective. Rbm14 complexed with proteins involved in RNA metabolism and phase separated into cellular ribonucleoprotein compartments. Consistently, RNA deep sequencing analysis on the morphant embryos revealed increased alternative splicing events as well as large-scale transcriptomic downregulations. Our results suggest that Rbm14 functions in ribonucleoprotein compartments through phase separation to modulate multiple aspects of RNA metabolism. Furthermore, IDRs conserve in phase separation ability but not primary sequence and can be functionally interchangeable.
Room-temperature superionic-phase nanocrystals synthesized with a twinned lattice
Authors: Gong Jianxiao , Jain Prashant K.
Published: July 2019

The engineering of nanoscale features enables the properties of solid-state materials to be tuned. Here, we show the tunable preparation of cuprous sulfide nanocrystals ranging in internal structures from single-domain to multi-domain. The synthetic method utilizes in-situ oxidation to grow nanocrystals with a controlled degree of copper deficiency. Copper-deficient nanocrystals spontaneously undergo twinning to a multi-domain structure. Nanocrystals with twinned domains exhibit markedly altered crystallographic phase and phase transition characteristics as compared to single-domain nanocrystals. In the presence of twin boundaries, the temperature for transition from the ordered phase to the high-copper-mobility superionic phase is depressed. Whereas the superionic phase is stable in the bulk only above ca. 100 °C, cuprous sulfide nanocrystals of ca. 7 nm diameter and a twinned structure are stable in the superionic phase well below ambient temperature. These findings demonstrate twinning to be a structural handle for nanoscale materials design and enable applications for an earth-abundant mineral in solid electrolytes for Li-S batteries.
Conditional expression explains molecular evolution of social genes in a microbe
Authors: de Oliveira Janaina Lima , Morales Atahualpa Castillo , Stewart Balint , Gruenheit Nicole , Engelmoer Jennifer , Brown Suzanne Battom , de Brito Reinaldo A. , Hurst Laurence D. , Urrutia Araxi O. , Thompson Christopher R. L. , Wolf Jason B.
Published: July 2019

Conflict is thought to play a critical role in the evolution of social interactions by promoting diversity or driving accelerated evolution. However, despite our sophisticated understanding of how conflict shapes social traits, we have limited knowledge of how it impacts molecular evolution across the underlying social genes. Here we address this problem by analyzing the genome-wide impact of social interactions using genome sequences from 67Dictyostelium discoideumstrains. We find that social genes tend to exhibit enhanced polymorphism and accelerated evolution. However, these patterns are not consistent with conflict driven processes, but instead reflect relaxed purifying selection. This pattern is most likely explained by the conditional nature of social interactions, whereby selection on genes expressed only in social interactions is diluted by generations of inactivity. This dilution of selection by inactivity enhances the role of drift, leading to increased polymorphism and accelerated evolution, which we call the Red King process.
Acoustic radiation pressure for nonreciprocal transmission and switch effects
Authors: Devaux Thibaut , Cebrecos Alejandro , Richoux Olivier , Pagneux Vincent , Tournat Vincent
Published: July 2019

Systems capable of breaking wave transmission reciprocity have recently led to tremendous developments in wave physics. We report herein on a concept that enables one-way transmission of ultrasounds, an acoustic diode, by relying on the radiation pressure effect. This effect makes it possible to reconfigure a multilayer system by significantly deforming a water-air interface. Such a reconfiguration is then used to achieve an efficient acoustic transmission in a specified direction of propagation but not in the opposite, hence resulting in a highly nonreciprocal transmission. The corresponding concept is experimentally demonstrated using an aluminum-water-air-aluminum multilayer system, providing the means to overcome key limitations of current nonreciprocal acoustic devices. We also demonstrate that this diode functionality can even be extended to the design and operations of an acoustic switch, thus paving the way for new wave control possibilities, such as those based on acoustic transistors, phonon computing and amplitude-dependent filters.
Pathogen transmission risk by opportunistic gulls moving across human landscapes
Authors: Navarro Joan , Grémillet David , Afán Isabel , Miranda Francisco , Bouten Willem , Forero Manuela G. , Figuerola Jordi
Published: July 2019

Wildlife that exploit human-made habitats hosts and spreads bacterial pathogens. This shapes the epidemiology of infectious diseases and facilitates pathogen spill-over between wildlife and humans. This is a global problem, yet little is known about the dissemination potential of pathogen-infected animals. By combining molecular pathogen diagnosis with GPS tracking of pathogen-infected gulls, we show how this knowledge gap could be filled at regional scales. Specifically, we generated pathogen risk maps of Salmonella, Campylobacter and Chlamydia based on the spatial movements of pathogen-infected yellow-legged gulls (Larus michahellis) equipped with GPS recorders. Also, crossing this spatial information with habitat information, we identified critical habitats for the potential transmission of these bacteria in southern Europe. The use of human-made habitats by infected-gulls could potentially increase the potential risk of direct and indirect bidirectional transmission of pathogens between humans and wildlife. Our findings show that pathogen-infected wildlife equipped with GPS recorders can provide accurate information on the spatial spread risk for zoonotic bacteria. Integration of GPS-tracking with classical epidemiological approaches may help to improve zoonosis surveillance and control programs.
The disappearing periglacial ecosystem atop Mt. Kilimanjaro supports both cosmopolitan and endemic microbial communities
Authors: Vimercati Lara , Darcy John L. , Schmidt Steve K.
Published: July 2019

Microbial communities have not been studied using molecular approaches at high elevations on the African continent. Here we describe the diversity of microbial communities from ice and periglacial soils from near the summit of Mt. Kilimanjaro by using both Illumina and Sanger sequencing of 16S and 18S rRNA genes. Ice and periglacial soils contain unexpectedly diverse and rich assemblages of Bacteria and Eukarya indicating that there may be high rates of dispersal to the top of this tropical mountain and/or that the habitat is more conducive to microbial life than was previously thought. Most bacterial OTUs are cosmopolitan and an analysis of isolation by geographic distance patterns of the genusPolaromonasemphasized the importance of global Aeolian transport in the assembly of bacterial communities on Kilimanjaro. The eukaryotic communities were less diverse than the bacterial communities and showed more evidence of dispersal limitations and apparent endemism. Cercozoa dominated the 18S communities, including a high abundance of testate amoebae and a high diversity of endemic OTUs within the Vampyrellida. These results argue for more intense study of this unique high-elevation “island of the cryosphere” before the glaciers of Kilimanjaro disappear forever.
Mapping the conductivity of graphene with Electrical Resistance Tomography
Authors: Cultrera Alessandro , Serazio Danilo , Zurutuza Amaia , Centeno Alba , Txoperena Oihana , Etayo David , Cordon Alvaro , Redo-Sanchez Albert , Arnedo Israel , Ortolano Massimo , Callegaro Luca
Published: July 2019

Electronic applications of large-area graphene films require rapid and accurate methods to map their electrical properties. Here we present the first electrical resistance tomography (ERT) measurements on large-area graphene samples, obtained with a dedicated measurement setup and reconstruction software. The outcome of an ERT measurement is a map of the graphene electrical conductivity. The same setup allows to perform van der Pauw (vdP) measurements of the average conductivity. We characterised the electrical conductivity of chemical-vapour deposited graphene samples by performing ERT, vdP and scanning terahertz time-domain spectroscopy (TDS), the last one by means of a commercial instrument. The measurement results are compared and discussed, showing the potential of ERT as an accurate and reliable technique for the electrical characterization of graphene samples.
C70 Fullerene Cage as a Novel Catalyst for Efficient Proton Transfer Reactions between Small Molecules: A Theoretical study
Authors: Varadwaj Pradeep R. , Varadwaj Arpita , Marques Helder M.
Published: July 2019

When acids are supplied with an excess electron (or placed in an Ar or the more polarizable N2 matrix) in the presence of species such as NH3, the formation of ion-pairs is a likely outcome. Using density functional theory and first-principles calculations, however, we show that, without supplying an external electron or an electric field, or introducing photo-excitation and -ionization, a single molecule of HCl or HBr in the presence of a single molecule of water inside a C70 fullerene cage is susceptible to cleavage of the σ-bond of the Brønsted-Lowry acid into X− and H+ ions, with concomitant transfer of the proton along the reaction coordinate. This leads to the formation of an X−···+HOH2 (X = Cl, Br) conjugate acid-base ion-pair, similar to the structure in water of a Zundel ion. This process is unlikely to occur in other fullerene derivatives in the presence of H2O without significantly affecting the geometry of the carbon cage, suggesting that the interior of C70 is an ideal catalytic platform for proton transfer reactions and the design of related novel materials. By contrast, when a single molecule of HF is reacted with a single molecule of H2O inside the C70 cage, partial proton transfers from HF to H2O is an immediate consequence, as recently observed experimentally. The geometrical, energetic, electron density, orbital, optoelectronic and vibrational characteristics supporting these observations are presented. In contrast with the views that have been advanced in several recent studies, we show that the encaged species experiences significant non-covalent interaction with the interior of the cage. We also show that the inability of current experiments to detect many infrared active vibrational bands of the endo species in these systems is likely to be a consequence of the substantial electrostatic screening effect of the cage.
Genomic and molecular characterisation of Escherichia marmotae from wild rodents in Qinghai-Tibet plateau as a potential pathogen
Authors: Liu Sha , Feng Jie , Pu Ji , Xu Xuefang , Lu Shan , Yang Jing , Wang Yiting , Jin Dong , Du Xiaochen , Meng Xiangli , Luo Xia , Sun Hui , Xiong Yanwen , Ye Changyun , Lan Ruiting , Xu Jianguo
Published: July 2019

Wildlife is a reservoir of emerging infectious diseases of humans and domestic animals. Marmota himalayana mainly resides 2800–4000 m above sea level in the Qinghai-Tibetan Plateau, and is the primary animal reservoir of plague pathogen Yersinia pestis. Recently we isolated a new species, Escherichia marmotae from the faeces of M. himalayana. In this study we characterised E. marmotae by genomic analysis and in vitro virulence testing to determine its potential as a human pathogen. We sequenced the genomes of the seven E. marmotae strains and found that they contained a plasmid that carried a Shigella-like type III secretion system (T3SS) and their effectors, and shared the same O antigen gene cluster as Shigella dysenterae 8 and E. coli O38. We also showed that E. marmotae was invasive to HEp-2 cells although it was much less invasive than Shigella. Thus E. marmotae is likely to be an invasive pathogen. However, E. marmotae has a truncated IpaA invasin, and lacks the environmental response regulator VirF and the IcsA-actin based intracellular motility, rendering it far less invasive in comparison to Shigella. E. marmotae also carried a diverse set of virulence factors in addition to the T3SS, including an IS1414 encoded enterotoxin gene astA with 37 copies, E. coli virulence genes lifA/efa, cif, and epeA, and the sfp gene cluster, Yersinia T3SS effector yopJ, one Type II secretion system and two Type VI secretion systems. Therefore, E. marmotae is a potential invasive pathogen.
Risk Factors Before Dialysis Predominate as Mortality Predictors in Diabetic Maintenance Dialysis patients
Authors: Tsur Noa , Menashe Idan , Haviv Yosef S.
Published: July 2019

Diabetic patients undergoing maintenance dialysis (MD) have a particularly high mortality rate. Many of the risk factors for mortality have been identified in diabetics who die before reaching end stage renal disease (ESRD), i.e. before dialysis (BD). In addition, many risk factors for mortality have been identified in diabetics after dialysis onset (AD). However, whether in the BD period there are long-term risk factors for AD mortality in diabetics is unknown. We therefore investigated a new concept, i.e. that clinical and biochemical risk factors during the BD stage affect long-term AD mortality. We performed a population based retrospective cohort study, in diabetic CKD patients in a single center in south Israel who initiated MD between the years 2003 and 2015. Clinical and biochemical data 12 months BD and 6 months AD were collected and evaluated for association with mortality AD using Cox’s proportional-hazards model. BD parameters that were found to be significant were adjusted for significant parameters AD, thus generating a “combined” regression model in order to isolate the contribution of BD factors on long term mortality. Six hundred and fifty two diabetic MD patients were included in the final analysis. Four independent BD parameters were found in the multivariate model to significantly predict AD mortality: age, BMI (inversely), pulse pressure (U-shaped) and cardiovascular comorbidity. AD independent risk factors for mortality were age, BMI (inversely) and albumin (inversely). Of note, BD factors remained dominantly significant even after additionally adjusting for AD factors. No association was found between either BD HbA1C levels or BD proteinuria and AD mortality. In diabetics who reach ESRD, BD parameters can predict long term AD mortality. Thus, some of the factors affecting the poor survival of diabetic MD patients appear to begin already in the BD period.
Recent increase in catastrophic tropical cyclone flooding in coastal North Carolina, USA: Long-term observations suggest a regime shift
Authors: Paerl Hans W. , Hall Nathan S. , Hounshell Alexandria G. , Luettich Richard A. , Rossignol Karen L. , Osburn Christopher L. , Bales Jerad
Published: July 2019

Coastal North Carolina, USA, has experienced three extreme tropical cyclone-driven flood events since 1999, causing catastrophic human impacts from flooding and leading to major alterations of water quality, biogeochemistry, and ecological conditions. The apparent increased frequency and magnitudes of such events led us to question whether this is just coincidence or whether we are witnessing a regime shift in tropical cyclone flooding and associated ecosystem impacts. Examination of continuous rainfall records for coastal NC since 1898 reveals a period of unprecedentedly high precipitation since the late-1990’s, and a trend toward increasingly high precipitation associated with tropical cyclones over the last 120 years. We posit that this trend, which is consistent with observations elsewhere, represents a recent regime shift with major ramifications for hydrology, carbon and nutrient cycling, water and habitat quality and resourcefulness of Mid-Atlantic and possibly other USA coastal regions.
Increased lymphocyte activation and atherosclerosis in CD47-deficient mice
Authors: Engelbertsen Daniel , Autio Anu , Verwilligen Robin A. F. , Depuydt Marie A. C. , Newton Gail , Rattik Sara , Levinsohn Erik , Saggu Gurpanna , Jarolim Petr , Wang Huan , Velazquez Francisco , Lichtman Andrew H. , Luscinskas Francis W.
Published: July 2019

CD47, also known as integrin-associated protein (IAP), is a transmembrane protein with multiple biological functions including regulation of efferocytosis and leukocyte trafficking. In this study we investigated the effect of CD47-deficiency on atherosclerosis using a model of adeno-associated virus (AAV)-induced hypercholesterolemia. We observed increased plaque formation in CD47 null mice compared to wild-type controls. Loss of CD47 caused activation of dendritic cells, T cells and natural killer (NK) cells, indicating an important role for CD47 in regulating immunity. In particular, Cd47 deficiency increased the proportion of IFN-γ producing CD90+ NK cells. Treatment with depleting anti-NK1.1 monoclonal antibody (mAb), but not depleting anti-CD4/CD8 mAbs, equalized atherosclerotic burden, suggesting NK cells were involved in the enhanced disease in Cd47 deficient mice. Additional studies revealed that levels of CD90+ and IFN-γ+ NK cells were expanded in atherosclerotic aorta and that CD90+ NK cells produce more IFN-γ than CD90- NK cells. Finally, we demonstrate that anti-CD47 (MIAP410) causes splenomegaly and activation of DCs and T cells, without affecting NK cell activation. In summary, we demonstrate that loss of CD47 causes increased lymphocyte activation that results in increased atherosclerosis.
Effects of carbon concentration on high-hardness plasma-polymer-fluorocarbon film deposited by mid-range frequency sputtering
Authors: Kim Sung Hyun , Kim Mac , Um Min Seop , Choi Woo Jin , Lee Jae Heung , Yang Yong Suk , Lee Sang-Jin
Published: July 2019

We propose a method for fabricating high-hardness plasma-polymer-fluorocarbon (PPFC) thin films with controllable optical and surface properties via manipulation of the target composition design and sputtering power density. The carbon/polytetrafluoroethylene (PTFE) composite polymeric material targets with the low electrical resistance were prepared by press-molding using a mechanically mixed powder of PTFE, carbon nanotubes, and graphite. The composite targets showed electrical sheet resistances of 0.1–100 Ω/sq. PPFC thin films were deposited by mid-range frequency (MF) sputtering at power densities within 0.62~4.92 W/cm2. The maximum surface hardness of the PPFC thin film was 4.75 GPa, which was 21.6 times higher than that of fluorocarbon thin film sputtered from PTFE under the same conditions. With the increase of the carbon concentration in the target, the carbon cross-linking density of the PPFC thin film increased but the fluorine concentration decreased. The concentration of fluorine in the PPFC thin films grew with increasing sputtering power density. The MF sputtered carbon-rich PPFC thin films are controllable with physical properties of optical transmittance, surface hardness and surface water repellency which could be applied as protective layers for transparent flexible devices.