bluuebunny/crossref_metadata_2025 huggingface dataset:
Unraveling Membrane-Disruptive Properties of Sodium Lauroyl Lactylate and Its Hydrolytic Products: A QCM-D and EIS Study
Authors: Gooran Negin , Tan Sue Woon , Yoon Bo Kyeong , Jackman Joshua A.
Published: May 2023

Membrane-disrupting lactylates are an important class of surfactant molecules that are esterified adducts of fatty acid and lactic acid and possess industrially attractive properties, such as high antimicrobial potency and hydrophilicity. Compared with antimicrobial lipids such as free fatty acids and monoglycerides, the membrane-disruptive properties of lactylates have been scarcely investigated from a biophysical perspective, and addressing this gap is important to build a molecular-level understanding of how lactylates work. Herein, using the quartz crystal microbalance–dissipation (QCM-D) and electrochemical impedance spectroscopy (EIS) techniques, we investigated the real-time, membrane-disruptive interactions between sodium lauroyl lactylate (SLL)—a promising lactylate with a 12-carbon-long, saturated hydrocarbon chain—and supported lipid bilayer (SLB) and tethered bilayer lipid membrane (tBLM) platforms. For comparison, hydrolytic products of SLL that may be generated in biological environments, i.e., lauric acid (LA) and lactic acid (LacA), were also tested individually and as a mixture, along with a structurally related surfactant (sodium dodecyl sulfate, SDS). While SLL, LA, and SDS all had equivalent chain properties and critical micelle concentration (CMC) values, our findings reveal that SLL exhibits distinct membrane-disruptive properties that lie in between the rapid, complete solubilizing activity of SDS and the more modest disruptive properties of LA. Interestingly, the hydrolytic products of SLL, i.e., the LA + LacA mixture, induced a greater degree of transient, reversible membrane morphological changes but ultimately less permanent membrane disruption than SLL. These molecular-level insights support that careful tuning of antimicrobial lipid headgroup properties can modulate the spectrum of membrane-disruptive interactions, offering a pathway to design surfactants with tailored biodegradation profiles and reinforcing that SLL has attractive biophysical merits as a membrane-disrupting antimicrobial drug candidate.
Palbociclib-Induced Cellular Senescence Is Modulated by the mTOR Complex 1 and Autophagy
Authors: Cayo Angel , Venturini Whitney , Rebolledo-Mira Danitza , Moore-Carrasco Rodrigo , Herrada Andrés A. , Nova-Lamperti Estefanía , Valenzuela Claudio , Brown Nelson E.
Published: May 2023

Despite not dividing, senescent cells acquire the ability to synthesize and secrete a plethora of bioactive molecules, a feature known as the senescence-associated secretory phenotype (SASP). In addition, senescent cells often upregulate autophagy, a catalytic process that improves cell viability in stress-challenged cells. Notably, this “senescence-related autophagy” can provide free amino acids for the activation of mTORC1 and the synthesis of SASP components. However, little is known about the functional status of mTORC1 in models of senescence induced by CDK4/6 inhibitors (e.g., Palbociclib), or the effects that the inhibition of mTORC1 or the combined inhibition of mTORC1 and autophagy have on senescence and the SASP. Herein, we examined the effects of mTORC1 inhibition, with or without concomitant autophagy inhibition, on Palbociclib-driven senescent AGS and MCF-7 cells. We also assessed the pro-tumorigenic effects of conditioned media from Palbociclib-driven senescent cells with the inhibition of mTORC1, or with the combined inhibition of mTORC1 and autophagy. We found that Palbociclib-driven senescent cells display a partially reduced activity of mTORC1 accompanied by increased levels of autophagy. Interestingly, further mTORC1 inhibition exacerbated the senescent phenotype, a phenomenon that was reversed upon autophagy inhibition. Finally, the SASP varied upon inhibiting mTORC1, or upon the combined inhibition of mTORC1 and autophagy, generating diverse responses in cell proliferation, invasion, and migration of non-senescent tumorigenic cells. Overall, variations in the SASP of Palbociclib-driven senescent cells with the concomitant inhibition of mTORC1 seem to depend on autophagy.
Physiological and Transcriptomic Analyses Reveal the Effects of Carbon-Ion Beam on Taraxacum kok-saghyz Rodin Adventitious Buds
Authors: Chen Xia , Du Yan , Luo Shanwei , Qu Ying , Jin Wenjie , Liu Shizhong , Wang Zhuanzi , Liu Xiao , Feng Zhuo , Qin Bi , Zhou Libin
Published: May 2023

Taraxacum kok-saghyz Rodin (TKS) has great potential as an alternative natural-rubber (NR)-producing crop. The germplasm innovation of TKS still faces great challenges due to its self-incompatibility. Carbon-ion beam (CIB) irradiation is a powerful and non-species-specific physical method for mutation creation. Thus far, the CIB has not been utilized in TKS. To better inform future mutation breeding for TKS by the CIB and provide a basis for dose-selection, adventitious buds, which not only can avoid high levels of heterozygosity, but also further improve breeding efficiency, were irradiated here, and the dynamic changes of the growth and physiologic parameters, as well as gene expression pattern were profiled, comprehensively. The results showed that the CIB (5–40 Gy) caused significant biological effects on TKS, exhibiting inhibitory effects on the fresh weight and the number of regenerated buds and roots. Then,15 Gy was chosen for further study after comprehensive consideration. CIB-15 Gy resulted in significant oxidative damages (hydroxyl radical (OH•) generation activity, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity and malondialdehyde (MDA) content) and activated the antioxidant system (superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and ascorbate peroxidase (APX)) of TKS. Based on RNA-seq analysis, the number of differentially expressed genes (DEGs) peaked at 2 h after CIB irradiation. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that DNA-replication-/repair- (mainly up-regulated), cell-death- (mainly up-regulated), plant-hormone- (auxin and cytokinin, which are related to plant morphogenesis, were mainly down-regulated), and photosynthesis- (mainly down-regulated) related pathways were involved in the response to the CIB. Furthermore, CIB irradiation can also up-regulate the genes involved in NR metabolism, which provides an alternative strategy to elevate the NR production in TKS in the future. These findings are helpful to understand the radiation response mechanism and further guide the future mutation breeding for TKS by the CIB.
Magnetic Nanostructures and Stem Cells for Regenerative Medicine, Application in Liver Diseases
Authors: Barreto da Silva Tatiane , Dias Evellyn Araújo , Cardoso Liana Monteiro da Fonseca , Gama Jaciara Fernanda Gomes , Alves Luiz Anastácio , Henriques-Pons Andrea
Published: May 2023

The term “liver disease” refers to any hepatic condition that leads to tissue damage or altered hepatic function and can be induced by virus infections, autoimmunity, inherited genetic mutations, high consumption of alcohol or drugs, fat accumulation, and cancer. Some types of liver diseases are becoming more frequent worldwide. This can be related to increasing rates of obesity in developed countries, diet changes, higher alcohol intake, and even the coronavirus disease 2019 (COVID-19) pandemic was associated with increased liver disease-related deaths. Although the liver can regenerate, in cases of chronic damage or extensive fibrosis, the recovery of tissue mass is impossible, and a liver transplant is indicated. Because of reduced organ availability, it is necessary to search for alternative bioengineered solutions aiming for a cure or increased life expectancy while a transplant is not possible. Therefore, several groups were studying the possibility of stem cells transplantation as a therapeutic alternative since it is a promising strategy in regenerative medicine for treating various diseases. At the same time, nanotechnological advances can contribute to specifically targeting transplanted cells to injured sites using magnetic nanoparticles. In this review, we summarize multiple magnetic nanostructure-based strategies that are promising for treating liver diseases.
Left Ventricular Ejection Fraction Association with Acute Ischemic Stroke Outcomes in Patients Undergoing Thrombolysis
Authors: Chee Ryan C. H. , Lin Norman H. , Ho Jamie S. Y. , Leow Aloysius S. T. , Li Tony Y. W. , Lee Edward C. Y. , Chan Mark Y. , Kong William K. F. , Yeo Tiong-Cheng , Chai Ping , Yip James W. L. , Poh Kian-Keong , Sharma Vijay K. , Yeo Leonard L. L. , Tan Benjamin Y. Q. , Sia Ching-Hui
Published: May 2023

(1) Background: Little is known about how left ventricular systolic dysfunction (LVSD) affects functional and clinical outcomes in acute ischemic stroke (AIS) patients undergoing thrombolysis; (2) Methods: A retrospective observational study conducted between 2006 and 2018 included 937 consecutive AIS patients undergoing thrombolysis. LVSD was defined as left ventricular ejection fraction (LVEF) < 50%. Univariate and multivariate binary logistic regression analysis was performed for demographic characteristics. Ordinal shift regression was used for functional modified Rankin Scale (mRS) outcome at 3 months. Survival analysis of mortality, heart failure (HF) admission, myocardial infarction (MI) and stroke/transient ischemic attack (TIA) was evaluated with a Cox-proportional hazards model; (3) Results: LVSD patients in comparison with LVEF ≥ 50% patients accounted for 190 and 747 patients, respectively. LVSD patients had more comorbidities including diabetes mellitus (100 (52.6%) vs. 280 (37.5%), p < 0.001), atrial fibrillation (69 (36.3%) vs. 212 (28.4%), p = 0.033), ischemic heart disease (130 (68.4%) vs. 145 (19.4%), p < 0.001) and HF (150 (78.9%) vs. 46 (6.2%), p < 0.001). LVSD was associated with worse functional mRS outcomes at 3 months (adjusted OR 1.41, 95% CI 1.03–1.92, p = 0.030). Survival analysis identified LVSD to significantly predict all-cause mortality (adjusted HR [aHR] 3.38, 95% CI 1.74–6.54, p < 0.001), subsequent HF admission (aHR 4.23, 95% CI 2.17–8.26, p < 0.001) and MI (aHR 2.49, 95% CI 1.44–4.32, p = 0.001). LVSD did not predict recurrent stroke/TIA (aHR 1.15, 95% CI 0.77–1.72, p = 0.496); (4) Conclusions: LVSD in AIS patients undergoing thrombolysis was associated with increased all-cause mortality, subsequent HF admission, subsequent MI and poorer functional outcomes, highlighting a need to optimize LVEF.
Incidence of Peri-Implantitis, Technical and Biological Complications of Single Implants Placed with Flap or Flapless Surgery—A 10–12-Year Case-Series
Authors: Naeini Emitis Natali , Atashkadeh Mandana , Jacquet Wolfgang , D’Haese Jan , De Bruyn Hugo
Published: May 2023

Background: Long-term follow-up of single implants and crowns is scarce, especially when inserted using flapless surgery. Aim: Evaluate survival, peri-implantitis incidence, and technical/biologic complications of solitary implants/crowns after 10–12 years of function. Material and methods: 49 patients with 53 single implants, initially operated with a one-stage flap (F) or flapless (FL) surgery and delayed loading, were recalled. Implant survival, radiographic bone-level changes compared to baseline, peri-implant health, and soft tissue aesthetics were registered. Differences in implant level between and within groups were statistically tested using the Mann–Whitney U-Test and Wilcoxon Signed Ranks Test, respectively. Results: 36 patients with 40 implants were reassessed, yielding 100% implant and 97.5% crown survival. The bone loss in F (n = 19) was 0.56 mm (SD 0.89; range −0.9–2.02) and −0.85 mm (SD 0.98; range −2.84–0.53) in FL (n = 21), indicative of bone gain in FL (p = 0.003), the latter due to a difference at baseline but bone-level was comparable (p = 0.126). Groups were comparable for probing pocket depth (PPD); (3.32 vs. 3.19 mm), Bleeding Index (BI); (0.15 vs. 0.22), and gingival recession; (0.38 vs. 0.17 mm). According to international criteria, the peri-implantitis incidence was 0%, but 32.5% of the implants/crowns experienced biological or technical complications irrespective of surgical technique. Conclusions: Solitary implants and crowns show good long-term clinical outcomes and peri-implant health. Flapless surgery is a good alternative to conventional in straightforward cases with sufficient bone volume and proper treatment planning.
Barotrauma during Noninvasive Respiratory Support in COVID-19 Pneumonia Outside ICU: The Ancillary COVIMIX-2 Study
Authors: Vetrugno Luigi , Deana Cristian , Castaldo Nadia , Fantin Alberto , Belletti Alessandro , Sozio Emanuela , De Martino Maria , Isola Miriam , Palumbo Diego , Longhini Federico , Cammarota Gianmaria , Spadaro Savino , Maggiore Salvatore Maurizio , Bassi Flavio , Tascini Carlo , Patruno Vincenzo ,   COVIMIX-STUDY Group
Published: May 2023

Background: Noninvasive respiratory support (NIRS) has been extensively used during the COVID-19 surge for patients with acute respiratory failure. However, little data are available about barotrauma during NIRS in patients treated outside the intensive care unit (ICU) setting. Methods: COVIMIX-2 was an ancillary analysis of the previous COVIMIX study, a large multicenter observational work investigating the frequencies of barotrauma (i.e., pneumothorax and pneumomediastinum) in adult patients with COVID-19 interstitial pneumonia. Only patients treated with NIRS outside the ICU were considered. Baseline characteristics, clinical and radiological disease severity, type of ventilatory support used, blood tests and mortality were recorded. Results: In all, 179 patients were included, 60 of them with barotrauma. They were older and had lower BMI than controls (p < 0.001 and p = 0.045, respectively). Cases had higher respiratory rates and lower PaO2/FiO2 (p = 0.009 and p < 0.001). The frequency of barotrauma was 0.3% [0.1–1.3%], with older age being a risk factor for barotrauma (OR 1.06, p = 0.015). Alveolar-arterial gradient (A-a) DO2 was protective against barotrauma (OR 0.92 [0.87–0.99], p = 0.026). Barotrauma required active treatment, with drainage, in only a minority of cases. The type of NIRS was not explicitly related to the development of barotrauma. Still, an escalation of respiratory support from conventional oxygen therapy, high flow nasal cannula to noninvasive respiratory mask was predictive for in-hospital death (OR 15.51, p = 0.001). Conclusions: COVIMIX-2 showed a low frequency for barotrauma, around 0.3%. The type of NIRS used seems not to increase this risk. Patients with barotrauma were older, with more severe systemic disease, and showed increased mortality.
Prevalence and Incidence of Atrial Fibrillation in Heart Failure with Mildly Reduced or Preserved Ejection Fraction: (Additive) Value of Implantable Loop Recorders
Authors: Gorter Thomas M. , van Veldhuisen Dirk J. , Mulder Bart A. , Artola Arita Vicente A. , van Empel Vanessa P. M. , Manintveld Olivier C. , Tieleman Robert G. , Maass Alexander H. , Vernooy Kevin , van Gelder Isabelle C. , Rienstra Michiel
Published: May 2023

Background: Atrial fibrillation (AF) is common in heart failure with mildly reduced or preserved ejection fraction (HFmrEF/HFpEF) and has a negative impact on outcome. Reliable data on prevalence, incidence, and detection of AF from contemporary, prospective HFmrEF/HFpEF studies are scarce. Methods: This was a prespecified sub-analysis from a prospective, multicenter study. Patients with HFmrEF/HFpEF underwent 12-lead electrocardiography (ECG), 24 h Holter monitoring, and received an implantable loop recorder (ILR) at the study start. During the 2 year follow-up, rhythm monitoring was performed via ILR, yearly ECG, and two yearly 24 h Holter monitors. Results: A total of 113 patients were included (mean age 73 ± 8 years, 75% HFpEF). At baseline, 70 patients (62%) had a diagnosis of AF: 21 paroxysmal, 18 persistent, and 31 permanent AF. At study start, 45 patients were in AF. Of the 43 patients without a history of AF, 19 developed incident AF during a median follow-up of 23 [15–25] months (44%; incidence rate 27.1 (95% confidence interval 16.3–42.4) per 100 person-years). Thus, after the 2-year follow-up, 89 patients (79%) had a diagnosis of AF. In 11/19 incident AF cases (i.e., 58%), AF was solely detected on the ILR. Yearly 12-lead ECG detected six incident AF cases and four of these cases were also detected on two yearly 24 h Holter monitors. Two incident AF cases were detected on an unplanned ECG/Holter. Conclusions: Atrial fibrillation is extremely common in heart failure with HFmrEF/HFpEF and may inform on symptom evaluation and treatment options. AF screening with an ILR had a much higher diagnostic yield than conventional modalities.
Perinatal Outcome of Singletons Born after Using a Simplified Low-Cost IVF Culture System and All Singletons Born in Flanders (Belgium) between 2012 and 2020
Authors: Ombelet Willem , Goemaes Régine , Fomenko Elizaveta , Campo Rudi
Published: May 2023

Background: We developed a simplified IVF culture system (SCS) which has proven to be effective and safe in a selected IVF cohort. Methods: Preterm birth (PTB) and low birth weight (LBW) of 175 singletons born after using the SCS, 104 after fresh embryo transfer (ET), and 71 after frozen embryo transfer, were compared with all singletons born in Flanders between 2012 and 2020 conceived after natural conception, ovarian stimulation (OS), and assisted reproduction (IVF/ICSI). Findings: The proportion of preterm (<37 weeks) births was significantly higher in the case of IVF or ICSI, followed by hormonal treatment, compared to spontaneous pregnancies. There was no significant difference in PTB between SCS and any of the other groups. Concerning the average birth weight we found no significant difference between singletons born after natural conception and SCS. However, a significant difference in average birth weight was found between SCS singletons and singletons born after IVF, ICSI and hormonal treatment, with a significantly higher birth weight in the SCS group. This difference was also observed in the proportion of babies weighing less than 2500 g, with significantly more LBW babies in the IVF and ICSI group compared to the SCS newborns. Interpretation: Taking into account the small series, PTB and LBW rates in SCS singletons were found to be comparable with singletons born after natural conception. Compared to babies born after ovarian stimulation and IVF/ICSI, SCS singletons had a lower PTB and LBW rates, although the differences were not significant for PTB. Our results confirm previous reports on reassuring perinatal outcomes after using the SCS technology.
Functional Biomaterials for Local Control of Orthodontic Tooth Movement
Authors: Lin Yi , Fu Moyu Lara , Harb Ingrid , Ma Lisa Xiaolu , Tran Simon D.
Published: May 2023

Orthodontic tooth movement (OTM) occurs with the application of a controlled mechanical force and results in coordinated tissue resorption and formation in the surrounding bone and periodontal ligament. The turnover processes of the periodontal and bone tissue are associated with specific signaling factors, such as Receptor Activator of Nuclear factor Kappa-β Ligand (RANKL), osteoprotegerin, runt-related transcription factor 2 (RUNX2), etc., which can be regulated by different biomaterials, promoting or inhibiting bone remodeling during OTM. Different bone substitutes or bone regeneration materials have also been applied to repair alveolar bone defects followed by orthodontic treatment. Those bioengineered bone graft materials also change the local environment that may or may not affect OTM. This article aims to review functional biomaterials that were applied locally to accelerate OTM for a shorter duration of orthodontic treatment or impede OTM for retention purposes, as well as various alveolar bone graft materials which may affect OTM. This review article summarizes various types of biomaterials that can be locally applied to affect the process of OTM, along with their potential mechanisms of action and side effects. The functionalization of biomaterials can improve the solubility or intake of biomolecules, leading to better outcomes in terms of increasing or decreasing the speed of OTM. The ideal timing for initiating OTM is generally considered to be 8 weeks post-grafting. However, more evidence is needed from human studies to fully understand the effects of these biomaterials, including any potential adverse effects.
Interaction of Rhus typhina Tannin with Lipid Nanoparticles: Implication for the Formulation of a Tannin–Liposome Hybrid Biomaterial with Antibacterial Activity
Authors: Sekowski Szymon , Naziris Nikolaos , Chountoulesi Maria , Olchowik-Grabarek Ewa , Czerkas Krzysztof , Veiko Artem , Abdulladjanova Nodira , Demetzos Costas , Zamaraeva Maria
Published: May 2023

Tannins are natural plant origin polyphenols that are promising compounds for pharmacological applications due to their strong and different biological activities, including antibacterial activity. Our previous studies demonstrated that sumac tannin, i.e., 3,6-bis-O-di-O-galloyl-1,2,4-tri-O-galloyl-β-D-glucose (isolated from Rhus typhina L.), possesses strong antibacterial activity against different bacterial strains. One of the crucial factors of the pharmacological activity of tannins is their ability to interact with biomembranes, which may result in the penetration of these compounds into cells or the realization of their activity on the surface. The aim of the current work was to study the interactions of sumac tannin with liposomes as a simple model of the cellular membrane, which is widely used in studies focused on the explanation of the physicochemical nature of molecule–membrane interactions. Additionally, these lipid nanovesicles are very often investigated as nanocarriers for different types of biologically active molecules, such as antibiotics. In the frame of our study, using differential scanning calorimetry, zeta-potential, and fluorescence analysis, we have shown that 3,6-bis-O-di-O-galloyl-1,2,4-tri-O-galloyl-β-D-glucose interacts strongly with liposomes and can be encapsulated inside them. A formulated sumac–liposome hybrid nanocomplex demonstrated much stronger antibacterial activity in comparison with pure tannin. Overall, by using the high affinity of sumac tannin to liposomes, new, functional nanobiomaterials with strong antibacterial activity against Gram-positive strains, such as S. aureus, S. epidermitis, and B. cereus, can be formulated.
The Forward Effect of Delayed Judgments of Learning Is Influenced by Difficulty in Memory and Category Learning
Authors: Wang Xun , Liu Xinyue , Chen Luyao , Feng Kaiqi , Ye Qun , Zhu Haoliang
Published: May 2023

Delayed judgment of learning (JOL) is a widely used metacognitive monitoring strategy that can also enhance learning outcomes. However, the potential benefits of delayed JOL on subsequent learning of new material, known as the forward effect of delayed JOL, and its stability and underlying mechanisms have yet to be fully explored. In this study, we investigated the forward effect of delayed JOL using previously unexamined word pair materials and explored the boundary conditions of this effect by manipulating the difficulty of the materials. We also examined this effect within the context of category learning. Our findings demonstrate that delayed JOL significantly enhanced the retention of new information (Experiment 1A), while the forward effect of the delayed JOL occurred only for material with a certain degree of difficulty rather than for easy material (Experiment 1B). These findings were extended and replicated using category learning (Experiment 2). These results suggest that delayed JOL can be used as a preparation strategy for subsequent learning, particularly when faced with challenging materials. Our study provides novel insights into the potential benefits and limitations of delayed JOL and contributes to our understanding of the underlying mechanisms that govern metacognitive monitoring and learning strategies.
The Atmospheric Input of Dissolvable Pb Based on the Radioactive 210Pb Budget in the Equatorial Western Indian Ocean
Authors: Lee Huisu , Lee Jaeeun , Lee Hyunmi , Kim Intae
Published: May 2023

To estimate the atmospheric deposition flux of 210Pb in the equatorial western Indian Ocean, we determined the dissolved (<0.45 μm) and particulate 210Pb (>0.45 μm) in the water column. In addition, we calculated the atmosphere-derived dissolvable Pb in seawater using the budget of 210Pb. The dissolved 210Pb and total 210Pb were higher in the surface layer and, overall, showed a decreasing distribution with depth. In particular, radioactive 210Pb activities in the surface-to-upper layer (<1000 m depth) were 1.5 to 2 times higher than those reported in the 1970s (in nearby regions), suggesting that there has been additional 210Pb input in recent years. Based on the mass balance of the total 210Pb budget in the water column, we estimated the atmospheric deposition flux of 210Pb and the residence time of Pb for the first time in this region. The atmospheric deposition flux of 210Pb was estimated to be 0.1–0.5 dpm cm−2 yr−1, and these values agreed with the general global estimations for the major oceans (0.1–0.7 dpm cm−2 yr−1). Considering the residence time of 210Pb (29–41 years) in the water column (estimated from the 210Pb inventory and 234Th-based Pb scavenging rate), the atmospheric input of seawater-dissolvable Pb was quantified to be 0.08–0.1 nmol cm−2 yr−1, which is about eight times higher than the estimated input in the early 1990s in the region. Therefore, these results imply that radioactive 210Pb could be a useful tracer for quantifying Pb flux in seawater.
Personalized Medicine in Ophthalmic Diseases: Challenges and Opportunities
Authors: Jin Kai , Zhang Chun
Published: May 2023

Personalized medicine is a broadly used term to encompass approaches used to tailor healthcare to the needs of individual patients [...]
Using Fetal Fibronectin Test to Reduce Hospital Admissions with Diagnosis of Preterm Labor: An Economic Evaluation Study
Authors: Amro Bedayah , Alhalabi Iman , George Anila , Haroun Hanan , Khamis Amar Hassan , Sawalhi Nadia Al
Published: May 2023

Background: Preterm labor and delivery remain a major problem in obstetrics accounting for perinatal morbidity and mortality. The challenge is to identify those with true preterm labor to avoid unnecessary hospital admissions. The fetal fibronectin (FFN) test is a strong predictor of preterm birth and can help identify women with true preterm labor. However, its cost-effectiveness as a strategy for triaging women with threatened preterm labor is still debatable. Objective: To evaluate the effect of FFN test implementation on hospital resources by reducing the admission rate of threatened preterm labor in a tertiary hospital, Latifa Hospital, UAE. Methods: A retrospective cohort study of singleton pregnancies between 24 and 34 weeks of gestation who attended Latifa Hospital in the period of September 2015–December 2016, complaining of threatened preterm labor after the availability of an FFN test, and a historical cohort study for those who attended with threatened preterm labor before the availability of an FFN test. Data analysis was performed using a Kruskal–Wallis test, Kaplan–Meier, Fischer exact chi-square and cost analysis. The significance was set at p-value < 0.05. Results: In total, 840 women met the inclusion criteria and were enrolled. The relative risk of FFN for delivery at term was 4.35 times higher among the negative-tested compared to preterm delivery (p-value < 0.001). A total of 134 (15.9%) women were unnecessarily admitted (FFN tested negative, delivered at term) which yielded $107,000 in extra costs. After the introduction of an FFN test, a 7% reduction of threatened preterm labor admissions was recorded.
Abandoned Intracardiac Electrodes in an Oncological Patient
Authors: Klotzka Aneta , Iwańczyk Sylwia , Sobańska Karolina , Mitkowski Przemysław , Woźniak Patrycja , Lesiak Maciej
Published: May 2023

Cardiological and oncological patients comprise the majority of patients admitted to the emergency unit with chronic or acute conditions that are the dominant cause of death worldwide. However, electrotherapy and implantable devices (pacemakers and cardioverters) improve the prognosis of cardiological patients. We present the case report of a patient who, in the past, had a pacemaker implanted due to symptomatic sick sinus syndrome (SSS) without removing the two remaining leads. Echocardiography revealed severe tricuspid valve regurgitation. The tricuspid valve septal cusp was in a restricting position due to the two ventricular leads passing through the valve. A few years later, she was diagnosed with breast cancer. We present a 65-year-old female admitted to the department due to right ventricular failure. The patient manifested symptoms of right heart failure, predominated by ascites and lower extremity edema, despite increasing doses of diuretics. The patient underwent a mastectomy two years ago due to breast cancer and was qualified for thorax radiotherapy. A new pacemaker system was implanted in the right subclavian area as the pacemaker generator occupied the radiotherapy field. In the case of right ventricular lead removal and the need for pacing and resynchronization therapy, guidelines allow a coronary sinus for LV pacing to avoid passing the leads through the tricuspid valve. We facilitated this approach in our patient, suggesting that the percentage of ventricular pacing was very low.
Switching from Risks to Opportunities: The Application of a Superbonus Tax Incentive to Heritage Buildings from the 1960s in Fragile Mountain Contexts
Authors: Gotta Alessia , Mecca Umberto , Rebaudengo Manuela
Published: May 2023

This paper introduces actual considerations for the progressive disuse of residential space in the Alpine territory, considering possible actions. Nowadays, the building complexes built around the 1960s and 1970s (a symbol of mass tourism) are suffering and searching for a new identity. The generation of owners who bought them has aged and the propensities of the new generations for holiday in those places has changed, which means fewer opportunities for leisure, particularly in the winter. Due to the great attention (and seeming opportunities) of current incentive policies toward improving the energy use of the existing stock, the authors investigate the private conveniences of transformations through the refurbishment of these buildings. Starting from a study of the territory and the dynamics of the local population, this research analyzes a possible set of energy works, based on a new (2020) incentive measure, the 110% Superbonus, which consists of a series of facilitation mechanisms, deductions, and reimbursements for building interventions. A large part of the insight is focused on a technical and economic feasibility study of the possible actions, following a process based on the evolution of the legislation. This work is based on a specific case study, located in a small municipality in the Piedmont mountain area, consisting of three apartment blocks of mostly second homes. The methodology adopted lends itself on the one hand, as a guide for preliminary economic energy assessments and, on the other hand, as a policy evaluation tool from the public and private perspectives.
A Phonological Study of Rongpa Choyul
Authors: Zheng Jingyao
Published: May 2023

This paper presents a detailed description of the phonology of the Rongpa variety of Choyul, an understudied Tibeto-Burman language spoken in Lithang (理塘) County, Dkarmdzes (甘孜) Tibetan Autonomous Prefecture of Sichuan Province, China. Based on firsthand fieldwork data, this paper lays out Rongpa phonology with details, examining its syllable canon, initial and rhyme systems, and word prosody. Peculiar characteristics of this phonological system are as follows: First, Rongpa has a substantial phonemic inventory, which comprises 43 consonants, 13 vowels, and 2 tones. 84 consonant clusters are observed to serve as the initial of a syllable. Secondly, the phonemic contrast between plain and uvularized vowels is attested. In addition, regressive vowel harmony on uvularization, height, and lip-roundedness can be clearly observed in various constructions including prefixed verb stems. Finally, regarding word prosody, two tones in monosyllabic words, /H/ and /L/, are observed to distinguish lexical meanings, and disyllabic words exhibit four surface pitch patterns. Pitch patterns in verb morphology are also examined. The findings and analyses as presented in this paper could form a foundation for future research on Rongpa Choyul.
Sodium-Glucose Co-Transporter 2 Inhibitors in Heart Failure—Current Evidence in Special Populations
Authors: Moady Gassan , Ben Gal Tuvia , Atar Shaul
Published: May 2023

Sodium-glucose co-transporter 2 (SGLT2) inhibitors, originally used for diabetes mellitus, are gaining more popularity for other indications, owing to their positive cardiovascular and renal effects. SGLT2 inhibitors reduce heart failure (HF) hospitalization and improve cardiovascular outcomes in patients with type 2 diabetes. Later, SGLT2 inhibitors were evaluated in patients with HF with reduced ejection fraction (HFREF) and had beneficial effects independent of the presence of diabetes. Recently, reductions in cardiovascular outcomes were also observed in patients with HF with preserved ejection fraction (HFPEF). SGLT2 inhibitors also reduced renal outcomes in patients with chronic kidney disease. Overall, these drugs have an excellent safety profile with a negligible risk of genitourinary tract infections and ketoacidosis. In this review, we discuss the current data on SGLT2 inhibitors in special populations, including patients with acute myocardial infarction, acute HF, right ventricular (RV) failure, left ventricular assist device (LVAD), and type 1 diabetes. We also discuss the potential mechanisms behind the cardiovascular benefits of these medications.
Impact of Chorionic Somatomammotropin In Vivo RNA Interference Phenotype on Uteroplacental Expression of the IGF Axis
Authors: Hord Taylor K. , Tanner Amelia R. , Kennedy Victoria C. , Lynch Cameron S. , Winger Quinton A. , Rozance Paul J. , Anthony Russell V.
Published: May 2023

While fetal growth is dependent on many factors, optimal placental function is a prerequisite for a normal pregnancy outcome. The majority of fetal growth-restricted (FGR) pregnancies result from placental insufficiency (PI). The insulin-like growth factors (IGF1 and IGF2) stimulate fetal growth and placental development and function. Previously, we demonstrated that in vivo RNA interference (RNAi) of the placental hormone, chorionic somatomammotropin (CSH), resulted in two phenotypes. One phenotype exhibits significant placental and fetal growth restriction (PI-FGR), impaired placental nutrient transport, and significant reductions in umbilical insulin and IGF1. The other phenotype does not exhibit statistically significant changes in placental or fetal growth (non-FGR). It was our objective to further characterize these two phenotypes by determining the impact of CSH RNAi on the placental (maternal caruncle and fetal cotyledon) expression of the IGF axis. The trophectoderm of hatched blastocysts (9 days of gestation, dGA) were infected with a lentivirus expressing either a non-targeting sequence (NTS RNAi) control or CSH-specific shRNA (CSH RNAi) prior to embryo transfer into synchronized recipient ewes. At ≈125 dGA, pregnancies were fitted with vascular catheters to undergo steady-state metabolic studies. Nutrient uptakes were determined, and tissues were harvested at necropsy. In both CSH RNAi non-FGR and PI-FGR pregnancies, uterine blood flow was significantly reduced (p ≤ 0.05), while umbilical blood flow (p ≤ 0.01), both uterine and umbilical glucose and oxygen uptakes (p ≤ 0.05), and umbilical concentrations of insulin and IGF1 (p ≤ 0.05) were reduced in CSH RNAi PI-FGR pregnancies. Fetal cotyledon IGF1 mRNA concentration was reduced (p ≤ 0.05) in CSH RNAi PI-FGR pregnancies, whereas neither IGF1 nor IGF2 mRNA concentrations were impacted in the maternal caruncles, and either placental tissue in the non-FGR pregnancies. Fetal cotyledon IGF1R and IGF2R mRNA concentrations were not impacted for either phenotype, yet IGF2R was increased (p ≤ 0.01) in the maternal caruncles of CSH RNAi PI-FGR pregnancies. For the IGF binding proteins (IGFBP1, IGFBP2, IGFBP3), only IGFBP2 mRNA concentrations were impacted, with elevated IGFBP2 mRNA in both the fetal cotyledon (p ≤ 0.01) and maternal caruncle (p = 0.08) of CSH RNAi non-FGR pregnancies. These data support the importance of IGF1 in placental growth and function but may also implicate IGFBP2 in salvaging placental growth in non-FGR pregnancies.
Test and Analysis of High-Permeability Material’s Microstructure in Magnetic Shielding Device
Authors: Zhou Weiyong , Sun Jinji , Han Bangcheng , Ren Jianyi , Li Yifei
Published: May 2023

The magnetic shielding device is used to provide an extreme weak magnetic field, which plays a key role in variety of fields. Since the high-permeability material constituting the magnetic shielding device determines the magnetic shielding performance, it is important to evaluate the property of the high-permeability material. In this paper, the relationship between the microstructure and the magnetic properties of the high-permeability material is analyzed using minimum free energy principle based on magnetic domain theory, and the test method of the material’s microstructure including the material composition, the texture and the grain structure to reflect the magnetic properties is put forward. The test result shows that the grain structure is closely related to the initial permeability and the coercivity, which is highly consistent with the theory. As a result, it provides a more efficient way to evaluate the property of the high-permeability material. The test method proposed in the paper has important significance in the high efficiency sampling inspection of the high-permeability material.
Experimental Study on Lap-Spliced Performance of High-Strength Stainless Steel Wire Mesh in Engineering Cementitious Composites
Authors: Zou Xuyan , Zhang Xiyuan , Li Ziyuan , Zhu Juntao
Published: May 2023

To investigate the mechanical properties of high-strength stainless steel wire mesh (HSSSWM) in Engineering Cementitious Composites (ECCs) and determine a reasonable lap length, a total of 39 specimens in 13 sets were designed and fabricated by considering the diameter of the steel strand, spacing of the transverse steel strand, and lap length. The lap-spliced performance of the specimens was tested through a pull-out test. The results revealed two failure modes in the lap connection of steel wire mesh in ECCs: pull-out failure and rupture failure. The spacing of the transverse steel strand had little effect on the ultimate pull-out force, but it restricted the slip of the longitudinal steel strand. A positive correlation was found between the spacing of the transverse steel strand and the slip amount of the longitudinal steel strand. With an increase in lap length, the slip amount and ‘lap stiffness’ to peak load increased, while the ultimate bond strength decreased. Based on the experimental analysis, a calculation formula for lap strength considering the correction coefficient β was established.
Comparative Study on Mechanical Property and Fracture Behavior of Age-Hardened LM4 Monolithic Composites Reinforced with TiB2 and Si3N4
Authors: Doddapaneni Srinivas , Sharma Sathyashankara , Chennegowda Gowrishankar Mandya , Shettar Manjunath , Hegde Ananda
Published: May 2023

The study aimed to compare and analyze the mechanical property and fracture behavior of LM4 composites reinforced with TiB2 (1–3 wt.%) and Si3N4 (1–3 wt.%) ceramic powders. A two-stage stir casting process was employed for the effective preparation of monolithic composites. To further enhance the mechanical properties of composites, a precipitation hardening treatment (both single (SSHT) and multistage (MSHT), followed by artificial aging at 100 and 200 °C) was conducted. From mechanical property tests, it was understood that in both the monolithic composites, the properties improved with an increase in wt.% of reinforcements, and composite samples subjected to MSHT + 100 °C aging treatment bested other treatments in terms of hardness and UTS values. Compared to as-cast LM4, there was a 32 and 150% increase in hardness and a 42 and 68% increase in UTS for as-cast and peak-aged (MSHT + 100 °C aging) LM4 + 3 wt.% TiB2 composites, respectively. Similarly, there was a 28 and 124% increase in hardness and a 34 and 54% increase in UTS for as-cast and peak-aged (MSHT + 100 °C aging) LM4 + 3 wt.% Si3N4 composites, respectively. Fracture analysis of the peak-aged composite samples confirmed the mixed mode of fracture in which brittle mode was dominating.
Study on Axial Dispersion Characteristics of Double-Layer Prefabricated Fragments
Authors: He Yuan , Guo Lei , Wang Chuanting , Du Jinyi , Wang Heng , He Yong
Published: May 2023

The axial distribution of initial velocity and direction angle of double-layer prefabricated fragments after an explosion were investigated via an explosion detonation test. A three-stage detonation driving model of double-layer prefabricated fragments was proposed. In the three-stage driving model, the acceleration process of double-layer prefabricated fragments is divided into three stages: “detonation wave acceleration stage”, “metal–medium interaction stage” and “detonation products acceleration stage”. The initial parameters of each layer of prefabricated fragments calculated by the three-stage detonation driving model of double-layer prefabricated fragments fit well with the test results. It was shown that the energy utilization rate of detonation products acting on the inner-layer and outer-layer fragments were 69% and 56%, respectively. The deceleration effect of sparse waves on the outer layer of fragments was weaker than that on the inner layer. The maximum initial velocity of fragments was located near the center of the warhead where the sparse waves intersected, located at around 0.66 times of the full length of warhead. This model can provide theoretical support and a design scheme for the initial parameter design of double-layer prefabricated fragment warheads.
Numerical Simulation of Physical Fields during Spark Plasma Sintering of Boron Carbide
Authors: Zhang Song , Liu Wei , Wang Weimin , Gao Ying , Wang Aiyang , He Qianglong , Bai Wenhui , Li Runfeng
Published: May 2023

Spark plasma sintering is a new technology for preparing ceramic materials. In this article, a thermal-electric-mechanical coupled model is used to simulate the spark plasma sintering process of boron carbide. The solution of the thermal-electric part was based on the charge conservation equation and the energy conservation equation. A phenomenological constitutive model (Drucker-Prager Cap model) was used to simulate the densification process of boron carbide powder. To reflect the influence of temperature on sintering performance, the model parameters were set as functions of temperature. Spark plasma sintering experiments were conducted at four temperatures: 1500 °C, 1600 °C, 1700 °C, and 1800 °C, and the sintering curves were obtained. The parameter optimization software was integrated with the finite element analysis software, and the model parameters at different temperatures were obtained through the parameter inverse identification method by minimizing the difference between the experimental displacement curve and the simulated displacement curve. The Drucker-Prager Cap model was then incorporated into the coupled finite element framework to analyze the changes of various physical fields of the system over time during the sintering process.
Enhanced Preservative Performance of Pine Wood through Nano-Xylan Treatment Assisted by High-Temperature Steam and Vacuum Impregnation
Authors: Fan Shutong , Gao Xun , Pang Jiuyin , Liu Guanlin , Li Xianjun
Published: May 2023

This study used environmentally friendly nano-xylan to enhance the drug loading and preservative performance (especially against white-rot fungi) of pine wood (Pinus massoniana Lamb), determine the best pretreatment, nano-xylan modification process, and analyze the antibacterial mechanism of nano-xylan. High-temperature, high-pressure steam pretreatment-assisted vacuum impregnation was applied to enhance the nano-xylan loading. The nano-xylan loading generally increased upon increasing the steam pressure and temperature, heat-treatment time, vacuum degree, and vacuum time. The optimal loading of 14.83% was achieved at a steam pressure and temperature of 0.8 MPa and 170 °C, heat treatment time of 50 min, vacuum degree of 0.08 MPa, and vacuum impregnation time of 50 min. Modification with nano-xylan prohibited the formation of hyphae clusters inside the wood cells. The degradation of integrity and mechanical performance were improved. Compared with the untreated sample, the mass loss rate of the sample treated with 10% nano-xylan decreased from 38 to 22%. The treatment with high-temperature, high-pressure steam significantly enhanced the crystallinity of wood.
Controlling the Magnetic Properties of La0.9A0.1Mn0.9Cr0.1O3 (A: Li, K, Na) Powders and Ceramics by Alkali Ions Doping
Authors: Głuchowski Paweł , Nikonkov Ruslan , Kujawa Daniela , Stręk Wiesław , Murauskas Tomas , Pakalniškis Andrius , Kareiva Aivaras , Yaremkevych Andrii , Fesenko Olena , Zhaludkevich Aliaksandr , Karpinsky Dmitry
Published: May 2023

Nanocrystalline La0.9A0.1Mn0.9Cr0.1O3 (A: Li, K, Na) powders have been synthesized by combustion method. The powders were used to prepare ceramics by high-pressure low-temperature sintering technique. For all samples the structure, elemental composition and morphology were studied using X-ray diffraction (XRD), Raman spectroscopy, Energy-Dispersive X-ray Spectroscopy (EDS) and Scanning electron microscopy (SEM). Magnetic properties were studied using magnetometry methods and the valency changes of the cations after alkali ions doping were studied using X-ray photoelectron spectroscopy (XPS). The influence of the sintering pressure on the structural and magnetic properties of the manganites doped with different alkali ions and chromium was also investigated. Magnetization properties were studied as a function of sintering pressure and type of the dopant. Chemical doping with alkali ions as well as external pressure significantly changed the magnetic properties of the compounds. It was found that the magnetic properties of the manganites could be predictably modified through the use of a suitable dopant element.
On Fractional-Order Discrete-Time Reaction Diffusion Systems
Authors: Almatroud Othman Abdullah , Hioual Amel , Ouannas Adel , Grassi Giuseppe
Published: May 2023

Reaction–diffusion systems have a broad variety of applications, particularly in biology, and it is well known that fractional calculus has been successfully used with this type of system. However, analyzing these systems using discrete fractional calculus is novel and requires significant research in a diversity of disciplines. Thus, in this paper, we investigate the discrete-time fractional-order Lengyel–Epstein system as a model of the chlorite iodide malonic acid (CIMA) chemical reaction. With the help of the second order difference operator, we describe the fractional discrete model. Furthermore, using the linearization approach, we established acceptable requirements for the local asymptotic stability of the system’s unique equilibrium. Moreover, we employ a Lyapunov functional to show that when the iodide feeding rate is moderate, the constant equilibrium solution is globally asymptotically stable. Finally, numerical models are presented to validate the theoretical conclusions and demonstrate the impact of discretization and fractional-order on system dynamics. The continuous version of the fractional-order Lengyel–Epstein reaction–diffusion system is compared to the discrete-time system under consideration.