bluuebunny/crossref_metadata_2025 huggingface dataset:
OPTIMISASI HEURISTIK TERHADAP JARINGAN KOMPLEKS
Authors: Nasution Devi Maiya Sari , Lubis Hariyati , Siregar Rika Permata Sari , Zarlis Muhammad , Efendi Syahril
Published: March 2022

Penelitian ini mengusulkan sebuah solusi matematika untuk masalah optimasi dengan melihat optimisasi heuristic terhadap jaringan kompleks. Optimisasi Heuristik yang diterapkan adalah Algoritma Ant Colony Optimization (ACO). Algoritma yang diadopsi dari perilaku koloni semut. Secara alamiah koloni semut mampu menemukan rute terpendek dalam perjalanan dari sarang ke tempat tempat sumber makanan. Koloni semuat dapat menemukan rute terpendek antara sarang dan sumber makanannya berdasarkan jejak kaki pada lintasan yang telah dilewati. Semakin banyak semut yang melewati suatu lintasan, maka akan semakin jelas bekas jejak kakinya. Algoritma Ant Colony Optimization (ACO) sangat tepat digunakan untuk diterapkan dalam penyelesaian masalah optimisasi, salah satunya adalah menentukan jalur terpendek yang diarahkan kedalam graf lengkap.
Investigation of salicylidene acylhydrazides derivatives: Molecular Docking, ADMET, and Molecular Dynamic Simulations were used in conjunction towards the design of new Yersinia pseudotuberculosis inhibitors
Authors: EDACHE Emmanuel , UZAİRU Adamu , MAMZA Paul Andrew , SHALLANGWA Gideon Adamu
Published: March 2022

LysR-type transcription factor RovM is an important target of Yersinia pseudotuberculosis drug discovery and the discovery of antibacterial is considered one of the greatest medical achievements of all time. In this research work, a combination of three docking tools with different algorithms was applied in Salicylidene acylhydrazides derivatives intended toward gram-negative bacterium Yersinia pseudotuberculosis to evaluate their binding interactions.
The analysis of the molecular docking results obtained from the 3-docking software system succeeded in screening twelve fascinating compounds with higher restrictive concentrations having a decent affinity to LysR-type transcription factor RovM macromolecule. Then the Lipinski’s and Veber’s rule properties were calculated to spot the drug-likeness properties of the investigated candidate compounds. To anticipate the toxicity of the predicted candidate chemicals, in-silico toxicity tests were conducted. Furthermore, golden triangle and drug scores were performed, the investigated compounds which fall within the golden triangle indicate that these compounds would not have clearance problems. 5 of the 12 hits drugs pass the golden triangle screening step. These selected drugs undergo a drug score test which only compound 17 passed. To validate the stability, 1 ns molecular dynamic simulations were done on the highest-ranking drug score compound 17 / 3onm complexes. These findings point to interesting avenues for the development of new compounds that are more effective against Yersinia pseudotuberculosis.
Konsep Pendidikan Iman dalam QS. Al-Mukminun
Authors: Dadan Rusmana  , Yayan Rahtikawati  , Mohamad Akbar Nugraha  , Muhammad Zia Alkhair  , Siham Adzan Al-Mujaddid
Published: March 2022

This paper discusses the concept of faith education in QS. Al-Mukminun, the focus of the study in this paper is the search for the concept of faith education contained in the QS. Al-Mu'minun. The writing method applied in this writing is the maudhu`i (thematic) interpretation method, by analyzing the verses of the Koran contained in the QS. Al-Mu'minun to find the concept of faith education contained in it. The steps of the maudhu`i interpretation method formulated by Abdul Hay Al-Farmawi. In this formulation there are seven steps in the maudhui interpretation, namely starting with determining the theme, and collecting verses related to the theme, then sorting the verses based on their historical context, then understanding the munasabah of the verses related to other verses, and compiling a perfect discussion framework, and completing discussion through quotations of related hadith, and the last step is to examine the related verses as a whole to find one concept that is studied. The data source in this article is the Koran and several secondary sources, such as the book Al-Mu'jam al-Mufahras li alfaadzi Al Qur'an by Muhammad Fuad Al-Baqi, several books and articles. This paper is a library research using a qualitative approach, which is trying to describe a variable, symptom or condition "as is", and is not intended to test certain hypotheses. The result of this paper is to find the concept of faith education contained in QS. Al-Mu'minun.
Ilmu Ma’ani dan Peranannya dalam Tafsir
Authors: Abdul Rohman  , Wildan Taufiq
Published: March 2022

This article is about ījāz, iṭnāb and musāwah in Qur’an, all three are part of tha ma’ani scinece of balaghah. The purose of writing this article is to find out the meaning of îjâz, ithnâb, dan musâwah and how it is applied in the verse of the Qur’an and what meaning it gives. The method used in this paper uses a qualitative method, sourced from written literature, ulûm Al-Quran, balâghah, tafser books, with a descriptive analysis approach, with decipter, analyze and describe it. The results obtained are that what is meant by îjâz in the study of ma’ani science is a sentence structure in which many meanings are collected and stated in a short editorial clearly and eloquently; ithnâb is the opposite it is adding a lafazh over a meaning for a specific purpose and it has seven forms. Meanhile what is meant by musâwah is disclosure of sentences whose meaning is in accordance with the number of words  and the number of words according to the breadth of meaning, no addition or subtraction. Each gives different meaning implications according to the context of the conversation and the will of the speaker of kalam. The purpose of their use is also different, provide an in depth explanation and in the context of the Qur’an verse is to provide a proof that the verse of the Qur’an is the Kalam Allah SWT.
Thermal Comfort Analysis of Moisture Management Treated Cotton Fabrics for Sportwear
Authors: Su Kyoung An
Published: March 2022

Currently, health concern continues to increase in importance and leads people to exercise more. It also increases people’s interest in purchasing athletic performance apparel that is promoted as superior apparel due to performance in moisture and heat transfer during exercising. This trend encourages more apparel companies to develop moisture management fabric. The aim of this study was to examine whether moisture management treated fabrics provide significant heat, moisture, and air transfers. Significant differences were found in air permeability among three different moisture management conditioned fabrics. There was no significant difference in heat and moisture transfers among them. However, statistically significant differences in heat, moisture, air transfers were found between two different fabric structures.
TEKNIK POLLING DI RECOMMENDER SYSTEM COLLABORATIVE FILTERING UNTUK PEMBELAJARAN DARING
Authors: Travada Eko
Published: March 2022

Currently, the Recommender System (RS) is a method that is widely used to help sort out information, which is currently very large. Without a Recommender System it will be very difficult to sort out the information one by one as needed. Sorting information in a RS is not the same as searching for information, as we do a search for files on storage media by simply writing a few keywords to find the files needed. RS sorting is by looking at the magnitude of a value obtained from drawing conclusions after analyzing the available data, either the user data itself or other user data. Information separation in online learning is also very much needed. Because online learning will be more effective if learners can be provided with the right material. Online learning that is currently available generally provides learning material content such as textbooks in hardcopy-like form. In this study, the online learning system was added with RS technology in order to help students choose the material that the user or students should study so that they can achieve the expected learning objectives. The method in the Recommender System that is widely researched in online learning is Collaborative Filtering. RS with collaborative filtering in order to provide accurate recommendations requires large amounts of data. Big data raises a problem, namely the spread of data occurs in many locations so that it requires complex computation in providing recommendations. To overcome the computational complexity, this paper will discuss the polling technique as a novelty in this study. The research shows that there is an increase in recommendation precision by 20%, when compared to data without polling.

Keywords: Recommender System, Collaborative Filtering, Polling
The Modernity of Poverty and Deprivation
Authors: KARAKUŞ Girayalp
Published: March 2022

The development of the media has radically changed the understanding of production and change. My criticism of the media is treated as a cultural element. Individuals use a technical tool for production. Media, on the other hand, is an object used for this purpose. It is necessary to state that such concepts as modernism, postmodernism and metamodernism have appeared in capitalist societies. These concepts have arisen due to the fact that the basic requirements of each culture are different. Modernism has been accused of being a status quoist approach while revealing pure reason. Although these criticisms have some justification, we can state that there are some extreme criticisms. Although concepts such as metamodernism and postmodernism were able to analyze modernity well, they could not replace modernism with an alternative system. Several subjective and objective relations are shaped by the market economy in modern societies. Therefore, a stereotype occurs in relations. In order to consolidate my theses, many domestic and foreign sources have been used in my study. Because of the subjective nature of social sciences, I tried to avoid definite judgments.The aim of the article is to explain how humanity has evolved within the framework of market economy rules.The aim of the article is to explain the concepts such as modernism, post-modernism and metamodernism and to reveal the role of the media.
Six New Theorems about Inscribed Triangle in a Circle
Authors: Mahmud Salman
Published: March 2022

In this paper we have proven 6 new theorems about inscribed triangle in a circle. If we extend the three medians of any inscribed triangle in a circle, the extended medians will intersect the circumference of the circle at three points. Connecting those three points we will get a new triangle. The main purpose of this paper is to describe the properties of the second triangle obtained by extending the medians of the first triangle.
Precursor Quantitation Methods for Next Generation Food Production
Authors: Wang Xinran , Luo Xiaozhou
Published: March 2022

Food is essential for human survival. Nowadays, traditional agriculture faces challenges in balancing the need of sustainable environmental development and the rising food demand caused by an increasing population. In addition, in the emerging of consumers’ awareness of health related issues bring a growing trend towards novel nature-based food additives. Synthetic biology, using engineered microbial cell factories for production of various molecules, shows great advantages for generating food alternatives and additives, which not only relieve the pressure laid on tradition agriculture, but also create a new stage in healthy and sustainable food supplement. The biosynthesis of food components (protein, fats, carbohydrates or vitamins) in engineered microbial cells often involves cellular central metabolic pathways, where common precursors are processed into different proteins and products. Quantitation of the precursors provides information of the metabolic flux and intracellular metabolic state, giving guidance for precise pathway engineering. In this review, we summarized the quantitation methods for most cellular biosynthetic precursors, including energy molecules and co-factors involved in redox-reactions. It will also be useful for studies worked on pathway engineering of other microbial-derived metabolites. Finally, advantages and limitations of each method are discussed.
Geographic and Ethnic Inequalities in Diabetes-Related Amputations
Authors: Bellia Alfonso , Meloni Marco , Andreadi Aikaterini , Uccioli Luigi , Lauro Davide
Published: March 2022

Individuals with diabetes mellitus are at increasing risk for major lower-extremity amputations (LEAs). Poor quality of life and remarkable disabilities are associated with LEAs, determining a high economic burden for the healthcare systems. Reducing LEAs is therefore a primary marker of quality of care of the diabetic foot. At global level, between-countries comparisons of LEAs rates are basically hampered by differences in criteria used for data collection and analysis among studies. Significant variability in amputation rates exists between geographic areas, and also within specific regions of a country. Overall 5-year mortality rate after major amputations is reported to vary substantially across countries, from 50 to 80%. The odds of LEAs are substantially higher for Black, Native American and Hispanic ethnicities compared with White groups, with similar figures observed in the economically disadvantaged areas compared to more developed ones. Such discrepancies may reflect differences in diabetes prevalence as well as in financial resources, health-care system organization and management strategies of patients with diabetic foot ulcers. Looking at the experience of countries with lower rates of hospitalization and LEAs worldwide, a number of initiatives should be introduced to overcome these barriers. These include education and prevention programs for the early detection of diabetic foot at primary care levels, and the multidisciplinary team approach with established expertise in the treatment of the more advanced stage of disease. Such a coordinated system of support for both patients and physicians is highly required to reduce inequalities in the odd of diabetes-related amputations worldwide.
Impact of Elevated Progesterone in Late Follicular Phase on Early Pregnancy Outcomes and Live Birth Rate After Fresh Embryo Transfers
Authors: Xu Yueming , Zhang Jie , Li Aimin , Yang Ni , Cui Na , Hao Guimin , Gao Bu-Lang
Published: March 2022

Objective: To investigate the effect of progesterone elevation during late follicular phase on early pregnancy outcomes and live births after fresh embryo transfers.

Methods: Patients who underwent IVF/ICSI treatment cycles were retrospectively enrolled. The effect of progesterone elevation was analyzed on early pregnancy outcome and live births after fresh embryo transfers.

Results: A total of 2,404 patients were enrolled on the day of HCG triggering (HCG0), 1,584 patients on the day before HCG triggering (HCG-1), and 800 patients 2 days before HCG triggering (HCG-2). With a 1 ng/ml increase in the progesterone level on HCG0 day when the progesterone level was ≥1.5 ng/ml, the clinical pregnancy rate decreased by 60% (95% CI: 0.2–0.7, p = 0.004), the intrauterine pregnancy rate decreased by 70% (95% CI: 0.2–0.7, p = 0.003), and the live birth rate decreased by 70% (95% CI: 0.1–0.7, p = 0.004). With a 1 ng/ml increase in the progesterone level on HCG-1 day, the clinical pregnancy rate decreased by 90% (95% CI: 0.0–0.5, p = 0.003) when the progesterone level was ≥1.6 ng/ml, the intrauterine pregnancy rate decreased by 90% (95% CI: 0.0–0.5, p = 0.001) when the progesterone was ≥1.5 ng/ml, and the live birth rate decreased by 90% (95% CI: 0.0–0.6, p = 0.015) when the progesterone was ≥1.7 ng/ml. On HCG-2 day when the progesterone was ≥1.2 ng/ml, the clinical pregnancy rate decreased by 80% (95% CI: 0.1–0.6, p = 0.003), and the intrauterine pregnancy rate decreased by 70% (95% CI: 0.1–0.7, p = 0.007) with a 1 ng/ml increase in the progesterone level.

Conclusion: Elevated progesterone level during the late follicular phase is an independent risk factor affecting the clinical pregnancy rate, intrauterine pregnancy rate, and live birth rate among infertile patients undergoing IVF/ICSI after fresh embryo transfers. When the progesterone level exceeds a certain level, the early pregnancy and live birth rates after fresh embryo transfers show a rapid downward trend.
Impact of Vimentin on Regulation of Cell Signaling and Matrix Remodeling
Authors: Ostrowska-Podhorodecka Zofia , Ding Isabel , Norouzi Masoud , McCulloch Christopher A.
Published: March 2022

Vimentin expression contributes to cellular mechanoprotection and is a widely recognized marker of fibroblasts and of epithelial-mesenchymal transition. But it is not understood how vimentin affects signaling that controls cell migration and extracellular matrix (ECM) remodeling. Recent data indicate that vimentin controls collagen deposition and ECM structure by regulating contractile force application to the ECM and through post-transcriptional regulation of ECM related genes. Binding of cells to the ECM promotes the association of vimentin with cytoplasmic domains of adhesion receptors such as integrins. After initial adhesion, cell-generated, myosin-dependent forces and signals that impact vimentin structure can affect cell migration. Post-translational modifications of vimentin determine its adaptor functions, including binding to cell adhesion proteins like paxillin and talin. Accordingly, vimentin regulates the growth, maturation and adhesive strength of integrin-dependent adhesions, which enables cells to tune their attachment to collagen, regulate the formation of cell extensions and control cell migration through connective tissues. Thus, vimentin tunes signaling cascades that regulate cell migration and ECM remodeling. Here we consider how specific properties of vimentin serve to control cell attachment to the underlying ECM and to regulate mesenchymal cell migration and remodeling of the ECM by resident fibroblasts.
Simultaneous Discrimination of Cys/Hcy and GSH With Simple Fluorescent Probe Under a Single-Wavelength Excitation and its Application in Living Cells, Tumor Tissues, and Zebrafish
Authors: Yan Dongling , Liu Likun , Liu Xiangbao , Liu Qi , Hou Peng , Wang Hao , Xia Chunhui , Li Gang , Ma Chunhui , Chen Song
Published: March 2022

Owing to the important physiological sits of biothiols (Cys, Hcy, and GSH), developing accurate detection methods capable of qualitative and quantitative analysis of biothiols in living systems is needed for understanding the biological profile of biothiols. In this work, we have designed and synthesized a 4′-hydroxy-[1,1′-biphenyl]-4-carbonitrile modified with NBD group-based fluorescent probe, BPN-NBD, for sensitive detection of Cys/Hcy and GSH by dual emission signals via a single-wavelength excitation. BPN-NBD exhibited an obvious blue fluorescence (λmaxem = 475 nm) upon the treatment with GSH and reacted with Cys/Hcy to give a mixed blue-green fluorescence (λmaxem = 475 and 545 nm). Meanwhile, BPN-NDB performed sufficient selectivity, rapid detection (150 s), high sensitivity (0.011 µM for Cys, 0.015 µM for Hcy, and 0.003 µM for GSH) and could work via a single-wavelength excitation to analytes and had the ability to image Cys/Hcy from GSH in living MCF-7 cells, tumor tissues, and zebrafish by exhibiting different fluorescence signals. Overall, this work provided a powerful tool for thiols visualization in biological and medical applications.
A Novel Trimethylamine Oxide-Induced Model Implicates Gut Microbiota-Related Mechanisms in Frailty
Authors: Chen Si-yue , Rong Xing-yu , Sun Xin-yi , Zou Yi-rong , Zhao Chao , Wang Hui-jing
Published: March 2022

Frailty is a complicated syndrome that occurs at various ages, with highest incidence in aged populations, suggesting associations between the pathogenesis of frailty and age-related changes. Gut microbiota (GM) diversity and abundance change with age, accompanied by increased levels of trimethylamine oxide (TMAO), a systemic inflammation-inducing GM metabolite. Thus, we hypothesized that TMAO may be involved in the development of frailty. We successfully established and verified a novel model of frailty in adult mice based on a 4-week intraperitoneal injection regime of TMAO followed by LPS challenge. The frailty index significantly increased in TMAO-treated mice after LPS challenge. TMAO also decreased claudin-1 immunofluorescent staining intensity in the jejunum, ileum, and colon, indicating that the destruction of intestinal wall integrity may increase vulnerability to exogenous pathogens and invoke frailty. 16S sequencing showed that TMAO significantly reduced the GM Firmicutes/Bacteroidetes (F/B) ratio, but not α-diversity. Interestingly, after LPS challenge, more genera of bacterial taxa were differently altered in the control mice than in the TMAO-treated mice. We infer that a variety of GM participate in the maintenance of homeostasis, whereas TMAO could blunt the GM and impair the ability to recover from pathogens, which may explain the continuous increase in the frailty index in TMAO-treated mice after LPS challenge. TMAO also significantly increased serum imidazole metabolites, and led to different patterns of change in serum peptide and phenylpropanoid metabolites after LPS stimulation. These changes indicate that glucose metabolism may be one mechanism by which GM inactivation causes frailty. In conclusion, TMAO leads to frailty by destroying intestinal barrier integrity and blunting the GM response.
Difference Analysis Between Canine Adenovirus Types 1 And 2
Authors: Zhu Yanzhu , Xu Jinfeng , Lian Shizhen , Zhang Rui , Hou Jinyu , Wang Minchun , Yan Xijun
Published: March 2022

Canine adenoviruses (CAdVs) include type 1 (CAdV-1, virulent strain) and type 2 (CAdV-2, attenuated strain). In recent years, the incidences of CAdV infections are increasing. However, they are difficult to distinguish when the symptoms are untypical. It is pivotal to find the differences between the two virus types for scientific, epidemiological, and specific treatment. CAdV-1 (virulent strain) and CAdV-2 (attenuated strain) induced canine hepatitis (ICH) and tracheobronchitis (ITB), respectively, but the clinical symptom is not obvious. CAdV-1 and CAdV-2 have the same genome structure, diameter, morphological features, and cytopathic features, but the same character hinder the diagnose time of the serotypes. CAdV-1 and CAdV-2 have a difference in the genome sequence, coding proteins, viral activity, hemagglutination patterns. After infection, pathogenicity and transmission route are different between the two serotypes. Sequence alignment, PCR, Real time-PCR assay are useful methods to distinguish the two serotypes. The attenuated live CAdV-2 vaccine is currently used to protect against CAdV-1, but it also has a risk. The further research should focus on the pathogenicity mechanism and the useful vaccine for the two serotypes of canine adenovirus.
Comparative Study on Concrete Crack Detection of Tunnel Based on Different Deep Learning Algorithms
Authors: Yu Hongliang , Zhu Lei , Li Dongbiao , Wang Qi , Liu Xiangyang , Shen Caihua
Published: March 2022

The computer vision inspection of surface cracks in concrete structures has the characteristics of convenient and efficient 24-h on-site inspection. In this paper, the instance segmentation method and semantic segmentation method are used to realize the surface crack recognition of concrete structures, and a concrete crack detection method based on two different deep learning methods is designed. Experiments show that in scenarios where detection accuracy is required, compared with the Mask Region with Convolutional Neural Network method, the U-Net model improves the model segmentation accuracy through up-sampling and skip connection, and its Recall, Kappa, and Dice are increased by 6.88, 1.94, and 7.72%, respectively. In the scene of positioning requirements, Mask Region with Convolutional Neural Network has a better detection effect than U-Net for very thin and inconspicuous cracks, effectively avoiding the situation of missing cracks.
A New Fluid Mobility Calculation Method Based on Frequency-Dependent AVO Inversion
Authors: Luo Xin , Chen Xuehua , Duan Yinghao , Chen Shizhen , Qi Yingkai , Huo Fei
Published: March 2022

Fluid mobility (i.e., permeability to viscosity ratio) is a key parameter that can evaluate the reservoir permeability and delineate the fluid characteristic in hydrocarbon-saturated reservoirs. Based on the asymptotic representation for the frequency-dependent reflections in the fluid-saturated pore-elastic media and frequency-dependent AVO inversion, we propose a novel method for estimating fluid mobility from poststack seismic data. First, we establish the relationship between fluid mobility and frequency-dependent AVO analysis. Then, the fluid mobility is estimated using the theory of frequency-dependent AVO inversion. Tests on synthetic data reveal that the fluid mobility shows excellent imageability for the fluid-saturated reservoirs and can accurately delineate the spatial distribution shape of the gas-saturated reservoir. The application of field data examples demonstrates that the fluid mobility calculated by the proposed method produces less background interferences caused by elastic layers compared with the conventional frequency-dependent fluid indicator. The frequency-dependent fluid mobility takes into account the dispersion features associated with hydrocarbon reservoirs, and it provides a new way to detect the location of hydrocarbon reservoirs and characterize their spatial distribution.
Vertical Variations of Submesoscale Motions Between New Jersey Shelf and Bermuda
Authors: Li Jianing , Wang Xin
Published: March 2022

Based on the measurements from the Oleander Project, the behaviors of submesoscale motions are examined in the area between New Jersey Shelf and Bermuda. The vertical variation of Rossby number, the kinetic energy in the submesoscale range, and the power law of kinetic energy spectra suggest that submesoscale motions are mainly confined within the surface mixed layer with seasonality that is strong in winter and weak in summer. Besides, submesoscale motions with no significant seasonality were also found beneath the surface mixed layer, which could reach 500 m depth. A possible explanation is that the drastically varying flows in the Gulf Stream and mesoscale eddy periphery could generate strong lateral shear throughout their influence depth, which is favorable for breaking the geostrophic balance and causing submesoscale motions beneath the surface mixed layer.
Case Report: A Patient With Neuroleptic Malignant Syndrome, Water Intoxication and Hyponatremia Associated With Severe Cerebral Edema and Coma
Authors: Takenouchi Haruka , Anno Takatoshi , Kimura Yukiko , Kawasaki Fumiko , Shirai Ryo , Kaneto Hideaki , Kurokawa Katsumi , Tomoda Koichi
Published: March 2022

Background

Water intoxication is typically caused by primary or psychogenic polydipsia that potentially may lead to fatal disturbance in brain functions. Neuroleptic malignant syndrome (NMS) is a serious complication induced by administration of antipsychotics and other psychotropic drugs. The combination of inappropriate secretion of antidiuretic hormone (SIDAH), NMS and rhabdomyolysis have been rarely reported. Our patient also developed severe water intoxication.

Case presentation

Herein we report a comatose case of NMS complicated with water intoxication, syndrome of SIADH and rhabdomyolysis. This patient had severe cerebral edema and hyponatremia that were improved rapidly by the correction of hyponatremia within a couple of days.

Conclusions

Malignant neuroleptic syndrome water intoxication, SIADH and rhabdomyolysis can occur simultaneously. Comatose conditions induced by cerebral edema and hyponatremia can be successfully treated by meticulous fluid management and the correction of hyponatremia.
Spatial Analysis of Agricultural Eco-Efficiency and High-Quality Development in China
Authors: Wang Guofeng , Mi Lingchen , Hu Jinmiao , Qian Ziyu
Published: March 2022

High-quality development has become a new requirement for China’s social and economic development. As an important industry related to the national economy and people’s livelihood, achieving high-quality development in agriculture has become the most urgent task currently facing agriculture. This study focuses on agricultural eco-efficiency to indicate spatial distribution of high-quality development based on agricultural input–output data from 2001 to 2019 and the SBM-Undesired model; this study focuses on Agricultural Eco-efficiency, a key indicator related to the high-quality development of agriculture, to measure the temporal and spatial evolution of Agricultural Eco-efficiency. The results show that the Agricultural Eco-efficiency has increased from 0.363 in 2001 to 0.818 in 2019, with a growth rate of 125.34%, and the provinces with higher agricultural eco-efficiency are mainly located in the eastern regions. In addition, there is a U-shaped change trend between Agricultural Eco-efficiency and the total output value of agriculture, forestry, animal husbandry, and fishery. In other words, the provinces with the total output value of agriculture, forestry, animal husbandry, and fishery in the low range and high range enjoy higher Agricultural Eco-efficiency. Compared with the eastern region, the middle reaches of Yellow River and middle reaches of Yangtze River have great potential to reduce carbon emissions. In order to achieve high-quality agricultural development, it is necessary to pay attention to key indicators for improving Agricultural Eco-efficiency, and the technology development of the central and western regions will be very useful to decrease the gap.
Getting to the Root of Organic Inputs in Groundwaters: Stygofaunal Plant Consumption in a Calcrete Aquifer
Authors: Saccò Mattia , Campbell Matthew A. , Nevill Paul , Humphreys William F. , Blyth Alison J. , Grierson Pauline F. , White Nicole E.
Published: March 2022

Groundwater environments interact with and support subterranean biota as well as superficial aquatic and terrestrial ecosystems. However, knowledge of subterranean energy flows remains incomplete. Cross-boundary investigations are needed to better understand the trophic structures of groundwater ecosystems and their reliance on carbon inputs from aboveground. In this study we used carbon and nitrogen stable isotope analyses combined with radiocarbon fingerprints to characterise organic flows in groundwater ecosystems. We coupled these data with DNA metabarcoding of the gut contents of stygofauna to further elucidate organic matter (OM) sources and shifts in diet preferences. Samples were collected from the arid zone Sturt Meadows calcrete aquifer under low rainfall (LR) and high rainfall (HR) conditions. Bayesian modelling of Δ14C, δ13C, and δ15N data indicated that primary consumers (copepods) incorporated mainly particulate organic carbon (POC) under LR but during HR shifted to root derived material (either exudates or direct root grazing). By contrast, diets of secondary consumers (amphipods) were dominated by root material under both LR and HR. Our DNA metabarcoding-based results indicate that amphipods relied primarily on root inputs from perennial trees (likely Eucalyptus and Callitris) during the dry season (LR). Under HR, diets of both amphipods and copepods also included organic material derived from a broad range of more shallow rooted shrubs, and ephemeral herbs and grasses. Our findings illustrate the complexity of functional linkages between groundwater biota and surface terrestrial ecosystems in environments where aboveground productivity, diversity and OM flux to groundwater are intimately linked to often episodic rainfall.
LYPD3, a New Biomarker and Therapeutic Target for Acute Myelogenous Leukemia
Authors: Hu Tingting , Zhang Yingjie , Yang Tianqing , He Qingnan , Zhao Mingyi
Published: March 2022

Background: Acute myelogenous leukemia (AML) is nosocomial with the highest pediatric mortality rates and a relatively poor prognosis. C4.4A(LYPD3) is a tumorigenic and high-glycosylated cell surface protein that has been proven to be linked with the carcinogenic effects in solid tumors, but no hematologic tumors have been reported. We focus on exploring the molecular mechanism of LYPD3 in the regulation of the occurrence and development of AML to provide a research basis for the screening of markers related to the treatment and prognosis.

Methods: Datasets on RNA Sequencing (RNA-seq) and mRNA expression profiles of 510 samples were obtained from The Cancer Genome Atlas Program/The Genotype-Tissue Expression (Tcga-gtex) on 10 March 2021, which included the information on 173 AML tumorous tissue samples and 337 normal blood samples. The differential expression, identification of prognostic genes based on the COX regression model, and LASSO regression were analyzed. In order to better verify, experiments including gene knockdown mediated by small interfering RNA (siRNA), cell proliferation assays, and Western blot were prefomed. We studied the possible associated pathways through which LYPD3 may have an impact on the pathogenesis and prognosis of AML by gene set enrichment analysis (GSEA).

Results: A total of 11,490 differential expression genes (DEGs) were identified. Among them, 4,164 genes were upregulated, and 7,756 genes were downregulated. The univariate Cox regression analysis and LASSO regression analysis found that 28 genes including LYPD3, DNAJC8, and other genes were associated with overall survival (OS). After multivariate Cox analysis, a total of 10 genes were considered significantly correlated with OS in AML including LYPD3, which had a poor impact on AML (p <0.05). The experiment results also supported the above conclusion. We identified 25 pathways, including the E2F signaling pathway, p53 signaling pathway, and PI3K_AKT signaling pathway, that were significantly upregulated in AML samples with high LYPD3 expression (p < 0.05) by GSEA. Further, the results of the experiment suggested that LYPD3 participates in the development of AML through the p53 signaling pathway or/and PI3K/AKT signaling pathway.

Conclusion: This study first proved that the expression of LYPD3 was elevated in AML, which was correlated with poor clinical characteristics and prognosis. In addition, LYPD3 participates in the development of AML through p53 or/and the PI3K/AKT signaling pathway.
Identification of Pathologic and Prognostic Genes in Prostate Cancer Based on Database Mining
Authors: Liu Kun , Chen Yijun , Feng Pengmian , Wang Yucheng , Sun Mengdi , Song Tao , Tan Jun , Li Chunyang , Liu Songpo , Kong Qinghong , Zhang Jidong
Published: March 2022

Background: Prostate cancer (PCa) is an epithelial malignant tumor that occurs in the urinary system with high incidence and is the second most common cancer among men in the world. Thus, it is important to screen out potential key biomarkers for the pathogenesis and prognosis of PCa. The present study aimed to identify potential biomarkers to reveal the underlying molecular mechanisms.

Methods: Differentially expressed genes (DEGs) between PCa tissues and matched normal tissues from The Cancer Genome Atlas Prostate Adenocarcinoma (TCGA-PRAD) dataset were screened out by R software. Weighted gene co-expression network analysis was performed primarily to identify statistically significant genes for clinical manifestations. Protein–protein interaction (PPI) network analysis and network screening were performed based on the STRING database in conjunction with Cytoscape software. Hub genes were then screened out by Cytoscape in conjunction with stepwise algorithm and multivariate Cox regression analysis to construct a risk model. Gene expression in different clinical manifestations and survival analysis correlated with the expression of hub genes were performed. Moreover, the protein expression of hub genes was validated by the Human Protein Atlas database.

Results: A total of 1,621 DEGs (870 downregulated genes and 751 upregulated genes) were identified from the TCGA-PRAD dataset. Eight prognostic genes [BUB1, KIF2C, CCNA2, CDC20, CCNB2, PBK, RRM2, and CDC45] and four hub genes (BUB1, KIF2C, CDC20, and PBK) potentially correlated with the pathogenesis of PCa were identified. A prognostic model with good predictive power for survival was constructed and was validated by the dataset in GSE21032. The survival analysis demonstrated that the expression of RRM2 was statistically significant to the prognosis of PCa, indicating that RRM2 may potentially play an important role in the PCa progression.

Conclusion: The present study implied that RRM2 was associated with prognosis and could be used as a potential therapeutic target for PCa clinical treatment.
Long-Term Outcomes After Percutaneous Transluminal Pulmonary Angioplasty in Patients With Takayasu Arteritis and Pulmonary Hypertension
Authors: Huang Zhiwei , Wang Man , Hu Fenghuan , Liu Xiaoning
Published: March 2022

Objective

To investigate the long-term efficacy of percutaneous transluminal pulmonary angioplasty (PTPA) in patients with Takayasu arteritis (TA) and pulmonary artery stenosis and pulmonary hypertension (PH).

Methods

Data from 183 lesions from 79 surgeries performed on 32 patients with TA and PH were analyzed. Symptoms, laboratory investigation results, World Health Organization (WHO) functional class, 6-min walk distance (6 MWD), hemodynamic parameters, and prognosis were analyzed at baseline and follow-up.

Results

The mean (± SD) age of the 32 patients (28 female, 4 male) was 42.8 ± 11.9 years, and the median follow-up was 49.5 months (interquartile range, 26–71 months). Compared with baseline, changes in total bilirubin, N-terminal pro-brain natriuretic peptide (NT-proBNP) level, 6 MWD, and WHO score functional class demonstrated significant differences (P<0.001). Echocardiography findings, right and left ventricular diameter, tricuspid annular plane systolic excursion, and estimated pulmonary artery systolic pressure were all improved (P=0.016, P<0.001, P<0.001, and P=0.005, respectively). Importantly, repeat right heart catheterization revealed that mean pulmonary artery pressure, pulmonary vascular resistance, and cardiac index also improved significantly at follow-up (P<0.001, P<0.001, and P=0.011, respectively). Pulmonary angiography revealed post-procedure restenosis in 64 (35.0%) lesions underwent PTPA within three to six months. Among three patients who underwent stent implantation, one experienced restenosis. Two patients died during the follow-up period, one from aggravation of right heart failure after lung infection, and the other in a traffic accident.

Conclusions

Results of this study indicated that PTPA significantly improved clinical symptoms, exercise tolerance, and hemodynamic parameters in patients with TA pulmonary artery stenosis and PH. More importantly, reperfusion pulmonary edema significantly decreased, and no patient died of PTPA-related complications with guidance from the pressure wire.
Harnessing Big Data, Smart and Digital Technologies and Artificial Intelligence for Preventing, Early Intercepting, Managing, and Treating Psoriatic Arthritis: Insights From a Systematic Review of the Literature
Authors: Bragazzi Nicola Luigi , Bridgewood Charlie , Watad Abdulla , Damiani Giovanni , Kong Jude Dzevela , McGonagle Dennis
Published: March 2022

Background

Rheumatological and dermatological disorders contribute to a significant portion of the global burden of disease. Big Data are increasingly having a more and more relevant role, being highly ubiquitous and pervasive in contemporary society and paving the way for new, unprecedented perspectives in biomedicine, including dermatology and rheumatology. Rheumatology and dermatology can potentially benefit from Big Data.

Methods

A systematic review of the literature was conducted according to the “Preferred Reporting Items for Systematic Reviews and Meta-Analyses” (PRISMA) guidelines, mining “Uno per tutti”, a highly integrated and automated tool/meta-database developed at the University of Genoa, Genoa, Italy, and consisting of 20 major scholarly electronic databases, including PubMed/MEDLINE. Big Data- or artificial intelligence-based studies were judged based on the modified Qiao’s critical appraisal tool for critical methodological quality assessment of Big Data/machine learning-based studies. Other studies designed as cross-sectional, longitudinal, or randomized investigations, reviews/overviews or expert opinions/commentaries were evaluated by means of the relevant “Joanna Briggs Institute” (JBI)’s critical appraisal tool for the critical methodological quality assessment.

Results

Fourteen papers were included in the present systematic review of the literature. Most of the studies included concerned molecular applications of Big Data, especially in the fields of genomics and post-genomics. Other studies concerned epidemiological applications, with a practical dearth of studies assessing smart and digital applications for psoriatic arthritis patients.

Conclusions

Big Data can be a real paradigm shift that revolutionizes rheumatological and dermatological practice and clinical research, helping to early intercept psoriatic arthritis patients. However, there are some methodological issues that should be properly addressed (like recording and association biases) and some ethical issues that should be considered (such as privacy). Therefore, further research in the field is warranted.

Systematic Review Registration

Registration code 10.17605/OSF.IO/4KCU2.
Cryopreservation of Sterlet, Acipenser ruthenus Spermatozoa: Evaluation of Quality Parameters and Fine Ultrastructure
Authors: Dadras Hadiseh , Golpour Amin , Rahi Deepali , Lieskovská Jaroslava , Dzyuba Viktoriya , Gazo Ievgeniia , Policar Tomas
Published: March 2022

The present study aimed to evaluate cryo-injury during the cryopreservation process in sterlet (Acipenser ruthenus) sperm, focusing on ultrastructural characteristics. Post-thaw sperm quality parameters, including total motility rate, curvilinear velocity (VCL), linearity (LIN), plasma membrane integrity, antioxidant status, DNA damage, and fine ultrastructure were examined on fresh and cryopreserved sperm with/without addition of a single optimal dose of AFPI (10 μg/mL). A lower motility rate, VCL and plasma membrane integrity, and increased DNA damage (p < 0.05) were observed in frozen-thawed spermatozoa with/without AFPI compared to fresh spermatozoa. The morphology and ultrastructure of spermatozoa were affected during the cryopreservation process with/without supplementation of AFPI. Morphological abnormalities were observed in mitochondria (49–54%) and flagellum (55–57%) of cryopreserved spermatozoa with/without AFPI compared to fresh spermatozoa. In conclusion, the morphology and ultrastructure of spermatozoa were slightly changed after cryopreservation of sterlet spermatozoa with/without 10 μg/mL AFPI.
Investigation on Natural Infection of Covert Mortality Nodavirus in Large Yellow Croaker, Larimichthys crocea
Authors: Xu Tingting , Fan Yuding , Jia Tianchang , Wang Chong , Wang Wei , Li Jun , Zhang Qingli , Yao Cuiluan
Published: March 2022

Covert mortality nodavirus (CMNV) recently became more prevalent, severely affecting farmed shrimps, and also other invertebrates and teleost fish, in the coastal areas of China. For better understanding of its prevalence and susceptibility of large yellow croaker Larimichthys crocea to CMNV, an epidemiological survey was carried out in the main producing areas (Ningbo, Zhejiang, China, and Ningde, Fujian, China) and its offshore feeding grounds in the Southern Yellow Sea. The collected cultured L. crocea individuals showed clinical symptoms such as anorexia and abnormal swimming and diagnosed for CMNV infection by using RT-nPCR assay. The positive rates for CMNV in the collected L. crocea samples from Ningbo, Ningde, and the Yellow Sea were 14.29% (4/28), 20.00% (7/35), and 16.67% (1/6), respectively. Furthermore, CMNV diagnosis using TaqMan probe-based reverse transcription quantitative PCR (TaqMan RT-qPCR) yielded positive rates of 25.00% (7/28), 22.86% (8/35), and 16.67% (1/6) respectively in the above-mentioned samples, and with a range of 12.73–3,108.33 of CMNV copy numbers/μg total RNA. Phylogenetic tree analysis based on the RNA-dependent RNA polymerase (RdRp) and capsid protein genes showed that CMNV isolates from L. crocea samples were clustered tightly with the original isolate of CMNV from Penaeus vannamei. The histopathological studies indicated that severe cellular necrosis occurred in the heart, liver, spleen, eye, and gill of naturally infected fish. Stronger positive signals for CMNV-specific probes appeared in the necrotic cells and tissues in the in situ hybridization (ISH) analysis. Transmission electron microscopy (TEM) revealed the presence of numerous 30 nm diameter CMNV-like viral particles. The purified CMNV suspension could cause a significant cytopathic effect (CPE) in epithelioma papilloma cyprini (EPC) cells and resulted in the formation of typical inclusion bodies, and also visible CMNV particles around the lysed EPC cells under the TEM. Taken together, all results of this study demonstrated that L. crocea is susceptible to CMNV. The prevalence of CMNV and its potential impacts on the wild population of L. crocea see the need for further investigations.
The Effect of Sacubitril/Valsartan Treatment on Cardiac and Renal Functions of a Patient With Cardiorenal Syndrome Type 4 and Stage 5 CKD After More Than Three Years of Follow-Up
Authors: Cheng Shuiqin , Zhou Tingting , Yu Le , Chen Yunmin , Zhang Zhihong , Wang Jinquan , Yu Yusheng
Published: March 2022

It is difficult to treat cardiorenal syndrome (CRS) in clinical practice, which is the common reason for the death of patients. This report aimed to describe the effects of sacubitril/valsartan treatment on cardiac and renal functions of a patient with cardiorenal syndrome type 4 (CRS4) after more than 3 years of follow-up. A 77-year-old Chinese woman was admitted to our hospital because of CRS4 and stage 5 chronic kidney disease (CKD), who had a history of long-term proteinuria and renal failure. The patient's cardiothoracic ratio (CTR) measured by chest X–ray was 0.6. Cardiac ultrasonography showed that the left ventricular ejection fraction (LVEF) was 0.40. The patient had been treated for heart failure (HF) for 5 months, but there was no improvement in clinical manifestations, and the renal function gradually deteriorated. In our hospital, she received sacubitril/valsartan treatment for at least 40 months. The symptoms of HF relieved, and the indices of cardiac function improved. In addition, the patient's renal function was stable. During the treatment, the dosage of sacubitril/valsartan needed to be adjusted to achieve the optimal therapeutic effect. Follow-up results showed that she achieved cardiac function of New York Heart Association (NYHA) class II with an ejection fraction of 0.60 and E/A > 1 indicated by echocardiogram, and did not develop hyperkalemia. In summary, the improvement of cardiac and renal functions of the CRS4 patient was associated with the long-term sacubitril/valsartan treatment.