bluuebunny/crossref_metadata_2025 huggingface dataset:
High-Content Small Molecule Screen Identifies a Novel Compound That Restores AP-4-Dependent Protein Trafficking in Neuronal Models of AP-4-Associated Hereditary Spastic Paraplegia
Authors: Saffari Afshin , Brechmann Barbara , Boeger Cedric , Saber Wardiya Afshar , jumo Hellen , Whye Dosh , Wood Delaney , Wahlster Lara , Alecu Julian , Ziegler Marvin , Scheffold Marlene , Winden Kellen , Hubbs Jed , Buttermore Elizabeth , Barrett Lee , Borner Georg , Davies Alexandra , Sahin Mustafa , Ebrahimi-Fakhari Darius
Published: June 2023

Unbiased phenotypic screens in patient-relevant disease models offer the potential to detect novel therapeutic targets for rare diseases. In this study, we developed a high-throughput screening assay to identify molecules that correct aberrant protein trafficking in adaptor protein complex 4 (AP-4) deficiency, a rare but prototypical form of childhood-onset hereditary spastic paraplegia, characterized by mislocalization of the autophagy protein ATG9A. Using high-content microscopy and an automated image analysis pipeline, we screened a diversity library of 28,864 small molecules and identified a lead compound, C-01, that restored ATG9A pathology in multiple disease models, including patient-derived fibroblasts and induced pluripotent stem cell-derived neurons. We used multiparametric orthogonal strategies and integrated transcriptomic and proteomic approaches to delineate putative molecular targets of C-01 and potential mechanisms of action. Our results define molecular regulators of intracellular ATG9A trafficking and characterize a lead compound for the treatment of AP-4 deficiency, providing important proof-of-concept data for future Investigational New Drug (IND)-enabling studies.
Investigating the continuous usage intention of app-based ride-sourcing services: An Extended Expectation–Confirmation Model
Authors: Elnadi Moustafa , Gheith Mohamed Hani
Published: June 2023

Despite the existence of several studies that focus on ride-sourcing services, most of them have concentrated on exploring users' pre-usage behaviour. This is one of the earliest studies that focused on exploring users’ post-usage behaviour. This paper aims to identify the significant factors that may affect existing consumers’ intentions to continue using of app-based ride-sourcing services in Egypt. Three robust models, namely the Expectation-Confirmation Model (ECM), the Technology Acceptance Model (TAM), and the Technology Readiness Model (TRM) were integrated and used as the theoretical framework for this study to accomplish this goal. An online questionnaire was used to collect the empirical data needed to test the hypotheses. The partial least squares structural equation modelling (PLS-SEM) technique has been used to evaluate the postulated relationships using data from 381 respondents. The results demonstrated the essential role that optimism, innovativeness, discomfort, and insecurity play in shaping users’ perceptions of ride-sourcing apps’ usefulness. The findings also showed that perceived ease of use was determined only by innovativeness and discomfort. Additionally, the findings confirmed that the continuance usage intention of ride-sourcing apps is significantly associated with users’ satisfaction level, perceived usefulness, and perceived ease of use. The results of this study will aid service providers in comprehending the variables influencing users' decisions to continue using ride-sourcing apps. Therefore, service providers can discover how to retain and satisfy their current users.
Identification of Lactate-Related Subgroups and Prognostic Model in Triple-Negative Breast Cancer
Authors: Huang ShanShan , Wu LinYu , Qiu Yu , Xie Yi , Wu Hao , Li YingQing , Xie XinHua
Published: June 2023

Background Triple-negative breast cancer (TNBC) is a highly aggressive subtype of breast cancer that exhibits elevated glycolytic capacity. Lactate, as a byproduct of glycolysis, is considered a major oncometabolite that plays an important role in oncogenesis and remodeling of the tumor microenvironment. However, the potential roles of lactate in TNBC are not yet fully understood. In this study, our goal was to identify prognosis-related lactate genes (PLGs) and construct a lactate-related prognostic model (LRPM) for TNBC.Methods First, we applied lactate-related genes to classify TNBC samples using hierarchical clustering algorithm. Then, we performed the log-rank analysis and the least absolute shrinkage and selection operator (LASSO) analysis to screen PLGs and construct the LRPM. The biological functions of the identified PLGs in TNBC were inverstigated using CCK8 assay and clone formation assay. Finally, we constructed a nomogram based on the lactate-risk score (LRS) and tumor clinical stage. We used operating characteristic (ROC) curve and decision curve analysis (DCA) to evaluate the predictive capability of the nomogram.Results Our results showed that the TNBC samples could be classified into two subgroups with different survival probabilities. Three genes (NDUFAF3, CARS2 and FH), which can suppress TNBC cell proliferation, were identified as PLGs. Moreover, the LRPM and nomogram exhibited excellent predictive performance for TNBC patient prognosis.Conclusion we have developed a novel LRPM that enables risk stratification and identification of poor molecular subtypes in TNBC patients, showing great potential in clinical practice.
Tobacco and Menthol flavored electronic cigarettes induced inflammation and dysregulated repair in lung fibroblast and epithelium
Authors: Wang Qixin , Lucas Joseph H , Pang Cortney , Zhao Ruogang , Rahman Irfan
Published: June 2023

Background
Electronic cigarette (e-cig) vaping has increased in the past decade in the US, and e-cig use is misleadingly marketed as a safe cessation for quitting smoking. The main constituents in e-liquid are humectants, such as propylene glycol (PG) and vegetable glycerine (VG), but different flavoring chemicals are also used. However, the toxicology profile of flavored e-cigs in the pulmonary tract is lacking. We hypothesized that menthol and tobacco-flavored e-cig (nicotine-free) exposure results in inflammatory responses and dysregulated repair in lung fibroblast and epithelium.
Method
We exposed lung fibroblast (HFL-1) and epithelium (BEAS-2B) to Air, PG/VG, menthol flavored, or tobacco-flavored e-cig, and determined the cytotoxicity, inflammation, and wound healing ability of the cells in a microtissue chip model.
Results
After exposure, HFL-1 showed decreased cell number with increased IL-8 levels in the tobacco flavor group compared to air. BEAS-2B also showed increased IL-8 secretion after PG/VG and tobacco flavor exposure, while menthol flavor exposure showed no change. Both menthol and tobacco-flavored e-cig exposure showed decreased protein abundance of type 1 collagen (COL1A1), α-smooth-muscle actin (αSMA), and fibronectin as well as decreased gene expression level of αSMA (Acta2) in HFL-1. After tobacco flavor e-cig exposure, HFL-1 mediated wound healing and tissue contractility were inhibited. Furthermore, BEAS-2B exposed to menthol flavor showed significantly decreased gene expression of CDH1, OCLN, and TJP1.
Conclusion
Overall, tobacco-flavored e-cig exposure induces inflammation in both epithelium and fibroblasts, and tobacco-flavored e-cig inhibits wound healing ability in fibroblast.
Soil microbial dark matter explored from genome-resolved metagenomics
Authors: Lu Caiyu , Wang Yiling , Yu Jingwen , Zhao Kankan , Xue Ran , Ren Hao , Lv Xiaofei , Pan Ronghui , Zhang Jia-Bao , Zhu Yong-Guan , Xu Jianming , Ma Bin
Published: June 2023

Soil contains a vast number of unidentified microbes, known as soil microbial dark matter. This is an important source of microbial biodiversity and genetic resources1,2, but has yet to be fully explored. In this study, we conducted the first large-scale excavation of soil microbial dark matter by reconstructing 40,039 metagenome-assembled genome bins (the SMAG catalog) from 2,990 soil metagenomes. We identified 16,530 of 21,077 species-level genome bins (SGBs) as unknown SGBs (uSGBs), which greatly expand archaeal and bacterial diversity across the tree of life. The uSGBs identified substantially increased the functional landscape and intra-species genome diversity of the soil microbiome, and provided large proportions of the 43,169 biosynthetic gene clusters and 8,544 CRISPR-Cas genes mined from the SMAG catalog. This suggests their potential for genetic resource exploitation. In addition, we determined that uSGBs contributed 84.6% of novel viral-host associations identified from the SMAG catalog. Our results demonstrate the richness of microbial dark matter in soil and highlight its potential in inspiring new avenues of research in the field of soil microbiology. We believe that the SMAG catalog will be of great value and interest to the broad readership, and will serve as a valuable genomic database to drive significant advances in understanding soil microbial biodiversity and exploiting novel genetic resources.
Prognostic value of venous outflow profiles on multiphase CT angiography for the patients with acute ischemic stroke after endovascular thrombectomy
Authors: Chu Yue , Yin Zi-Xin , Ni Wen-Jing , Lu Shan-Shan , Shi Hai-Bin , Liu Sheng , Wu Fei-Yun , Xu Xiao-Quan
Published: June 2023

Objectives: To evaluate the prognostic value of venous outflow (VO) profiles evaluated on multiphase CTA (mCTA) for the patients with acute ischemic stroke (AIS) after endovascular thrombectomy (EVT).
Methods: We retrospectively collected 150 patients with AIS who underwent pre-treatment CT perfusion (CTP) evaluation and subsequent EVT in our center from April 2018 to April 2022. Three-phases (peak arterial phase, peak venous phase, late venous phase) CTA was reconstructed from CTP raw data, and VO was evaluated on three-phases CTA, respectively. Favorable VO was regarded as a cortical vein opacification score of 3–6, and unfavorable VO as a score of 0–2. Good outcome was defined as modified Rankin Scale score of 0-2 at 90 days after EVT. Multivariate logistic regression analysis was performed to explore the predictors of good outcome. Prognostic value was assessed and compared using receiver operating characteristic (ROC) curves and Delong test.
Results: Good outcome was achieved in 85 (56.7%) patients. Among the mCTA- derived VO profiles, only favorable peak venous phase VO was found to be independently associated with good outcome (P<0.001). After integrating favorable peak venous phase VO with lower post-treatment National Institute of Health Stroke Scale score at 24 hours, successful recanalization and favorable hypoperfusion intensity ratio, the predictive ability for a good outcome was significantly improved than before (area under the ROC curve; 0.947 vs 0.881; P=0.002).
Conclusions: Favorable peak venous VO profiles on mCTA might be a promising biomarker in predicting the good outcome in patients with AIS after EVT.
Time-series analysis of temperature variability and cardiovascular emergency department visits in Atlanta over a 27-year period
Authors: Lane Morgan , Ebelt Stefanie , Wu Zhen , Scovronick Noah , D'Souza Rohan , Chang Howard
Published: June 2023

Background
Short-term temperature variability, defined as the temperature range occurring within a short time span at a given location, appears to be increasing with climate change. Such variation in temperature may influence acute health outcomes, especially cardiovascular diseases (CVD). Most research on temperature variability has focused on the impact of within-day diurnal temperature range, but temperature variability over a period of a few days may also be health-relevant through its impact on thermoregulation and autonomic cardiac functioning. To address this research gap, this study utilized a database of emergency department (ED) visits for a variety of cardiovascular health outcomes over a 27-year period to investigate the influence of three-day temperature variability on CVD.
Methods
For the period of 1993–2019, we analyzed over 12 million CVD ED visits in Atlanta using a Poisson log-linear model with overdispersion. Temperature variability was defined as the standard deviation of the minimum and maximum temperatures during the current day and the previous two days. We controlled for mean temperature, dew point temperature, long-term time trends, federal holidays, and day of week. We stratified the analysis by age group, season, and decade.
Results
All cardiovascular outcomes assessed, except for hypertension, were positively associated with increasing temperature variability, with the strongest effects observed for stroke and peripheral vascular disease. In stratified analyses, adverse associations with temperature variability were consistently highest in the moderate-temperature season (October and March-May) and in the 65 + age group for all outcomes.
Conclusions
Our results suggest that CVD morbidity is impacted by short-term temperature variability, and that patients aged 65 and older are at increased risk. These effects were more pronounced in the moderate-temperature season and are likely driven by the Spring season in Atlanta. Public health practitioners and patient care providers can use this knowledge to better prepare patients during seasons with high temperature variability or ahead of large shifts in temperature.
Sickle Cell Disease and the Heart
Authors: Akivis Yonatan , Seidman Ira , Salciccioli Louis , McFarlane Samy I. , Wengrofsky Perry , Muthu John , Budzikowski Adam , Khatun Nazima , John Sabu
Published: June 2023

Purpose
The objective of this study was to explore the relationship between cardiac structure, cardiac index (CI), and diastolic function parameters, and laboratory values in patients with Sickle Cell Disease (SCD), and to characterize the distinct SCD cardiomyopathy phenotype
Methods
We conducted a retrospective review of 202 adult patients with SCD (mean age 41.02 ± 13.36) at our hospital who underwent outpatient echocardiographic screening from 2019–2022.
Results
Our study identified cardiac hypertrophy, chamber dilatation with preserved ejection fraction, elevated TRV, increased cardiac output, and diastolic dysfunction as defining echocardiographic features in SCD. There was a negative correlation between CI and lateral e’ (R = -0.182, P = 0.012), and a positive correlation between CI and E/e’ (R = 0.274, P = 0.0001).
Conclusions
Our study identified a unique cardiomyopathy in patients with SCD characterized by cardiac hypertrophy with preserved systolic function, abnormal mitral inflow patterns, and elevated cardiac output. These features are indicative of restrictive physiology, as evidenced by left atrial enlargement and diastolic dysfunction, superimposed on hyperdynamic physiology. Further research is needed to elucidate the pathophysiological mechanisms underlying these observations and determine their prognostic significance in vulnerable populations with an elevated burden of cardiovascular disease, including the risk of sudden cardiac death.
Adaptive MIMO-FSO Space Diversity MRC Reception Characteristics over Malaga Turbulence Channel with Pointing Errors
Authors: CHEN DAN , Ai Feier , Wang Chenhao , Zhao Peiyan , Lu Mengmeng
Published: June 2023

Atmospheric turbulence channels experience quasi-static fading, making it relatively easy to use feedback information for link adaptive transmission. Applying adaptive modulation to MIMO systems can effectively reduce the degradation of FSO signals under adverse turbulence conditions. In this paper, considering the joint influence of pointing error and Malaga distribution turbulence fading, the probability density function (PDF) of the received light intensity of MIMO channel employing maximum ratio combining (MRC) is derived based on the generalized power series. Using the cumulative density function (CDF)-based method, We analyze the asymptotic spectral efficiency(SE) and average bit error rate (BER) performance of the adaptive MPSK-MIMO systemunder different turbulence intensities, beam waist radius, jitter standard deviation and aperture number. In addition, we compare the BER performance of adaptive with non adaptive MIMO systems using MRC receiver. Monte Carlo simulation further verify our analysis. The derived results help quantifying the diversity order of our considered systems.
Narayana’s cows numbers which are concatenations of three repdigits in base ρ
Authors: Tiebekabe Pagdame , Ad\'edji Kou\`essi Norbert
Published: June 2023

\noindent Narayana's sequence is a ternary recurrent sequence defined by the recurrence relation $\mathcal{N}_n=\mathcal{N}_{n-1}+\mathcal{N}_{n-3}$ with initial terms $\mathcal{N}_0=0$ and $\mathcal{N}_1=\mathcal{N}_2=\mathcal{N}_3=1$. Let $\rho\geqslant2$ be a positive integer. In this study, it is proved that the $n$th Narayana number $ \mathcal{N}_n$ which are concatenations of three repdigits in base $\rho$ satisfies $n<5.6\cdot 10^{48}\cdot \log^7\rho$. Moreover, it is shown that the largest Narayana's number which are concatenations of three repdigits in base $\rho$ with $1\leqslant \rho\leqslant 10$ is $58425=\mathcal{N}_{31}=\overline{3332200}_5=\overline{332223}_7 .$
Combined use of sheep manure and di-ammonium phosphate mitigates adverse effects of nitrogen and phosphorus deficiencies and enhance growth, photosynthesis, and yield of soybean under Parwan agro-climatic conditions
Authors: Osmani Abdul Alim , Amin Mohammad Wasif , Farid Zabihullah , Habibi Nasratullah , Aryan Shafiqullah , Sediqui Naveedullah , Joya Khalid , Hazim Habibullah , Younisi Hamidullah , Amini Hakimullah
Published: June 2023

Severe climate changes in arid and semi-arid regions, for example rising summer temperatures, lowering winter temperatures, and a lack of rainfall, have made calcareous soils' fertility concerns, more specifically nitrogen and phosphorus deficits, progressively worse. Moreover, there is limited information available regarding how sheep manure (SM) and di ammonium phosphate (DAP) affect soybean growth, photosynthesis, and yield, particularly under calcareous soil conditions. This study investigated the effects of sole and combined application of SM and DAP fertilizer namely FT1 (SM = 0%, DAP= 0%), FT2 (SM= 100%, DAP= 0%), FT3 (SM = 0%, DAP= 100%), FT4 (SM = 50%, DAP= 75%), FT5 (SM = 50%, DAP= 50%), and FT6 (SM = 50%, DAP= 0%) on growth, photosynthesis, and yield parameters of soybean cultivar (LD 04-13265 USD) in calcareous soil of a semiarid region in Parwan, Afghanistan. The results indicated that plant height and nodule number were unaffected by fertilization treatments, however growth features including leaf number, root length, and shoot biomass affected significantly. The net photosynthetic rate, stomatal conductance, transpiration rate, and water use efficiency percentage were found to be significantly higher in FT4 and FT5, compared to FT1 (control). Conversely, each fertilization had a lower intercellular CO2 concentration than FT1. As a result, combined application of SM and DAP fertilizations such as FT4 and FT5 revealed better growth performance, higher photosynthesis efficiency as well as producing more seed yield compared to sole fertilizations. The increased growth, efficient photosynthesis and higher seed yield could be attributed to some extent to support nutrient availability, soil fertility, balanced nutrition, and a favourable soil environment by incorporating SM and DAP fertilizer. Ongoing studies can investigate the long-term effects of combining SM and DAP fertilizer on soil health parameters in calcareous soils. This can include assessing changes in soil pH, organic matter content, microbial activity, and nutrient cycling processes. Understanding these dynamics will contribute to sustainable agricultural practices and long-term soil fertility improvement.
Teacher students’ legitimation of teaching activities in the L1 classroom
Authors: Elvebakk Lisbeth , Skarstein Dag
Published: June 2023

This study investigates Norwegian L1 teacher students’ legitimation language as they retrospectively reflect on internship experiences during their first two years of teacher education. We aim to describe the teacher students’ legitimation of teaching activities within the framework of the specialization dimension of Legitimation Code Theory. Drawing particularly on Bernstein’s ideas on different knowledge structures and on teaching and acquisition of humanistic subjects, we critically discuss why the interviewees’ legitimation language only to a small degree works to classify L1 as a distinct school subject. By investigating the legitimation language used by teacher students in their second year of teacher education, our study aims to highlight the educational pathway to the L1 teacher profession. Using policy ideals as a backdrop for discussing the implications of our findings, we describe possible measures to assist teacher students’ development toward professional subject didactic reasoning and legitimation.
Why should the carbon tax be floating? A Tobin’s Q model applied to green investment
Authors: Piluso Nicolas
Published: June 2023

The carbon market reform is controversial because the modalities of carbon pricing foreseen risk reducing the performance of companies and negatively affecting the economy. The objective of this paper is to show that the carbon tax can be floating and adapt to the economic situation while maintaining its ecological efficiency. Herein, Tobin’s Q model, which has become a standard in the literature for explaining the investment decision, is applied to the green investment decision. A carbon tax is introduced into the firm’s maximization program to see how carbon pricing changes the outcome of the traditional model. The model shows that green investment depends on the sum of the stock price and the carbon price, which suggests the possibility of modulating this amount according to the upward or downward trend of the stock price to avoid permanently penalizing the competitiveness of firms. The study also demonstrates how the financial market is likely to value green investments and that such investments will likely generate shareholder value through several channels. Indeed, green investments impact the firm’s turnover and the minimum income required by the shareholder. Such a modulation of the carbon tax according to the economic cycle would make reconciling ecological and economic efficiency possible.
Social Media and Word of Mouth Effects on Buying Decision Process on Kopi Janji Jiwa in East Jakarta
Authors: Nicolas Nicolas , Widiartanto Widiartanto , Susanta Nugraha Hari
Published: June 2023

The research aimed to analyze consumer behavior in particular, consumer buying decision from Kopi Janji Jiwa. The research focused on the buying decision process from consumers, including the influence of Instagram promotion and word of mouth on buying decisions, which is mediated by purchase intention and brand awareness from Kopi Janji Jiwa in East Jakarta. The research applied a quantitative method. The survey was conducted through questionnaires on Kopi Janji Jiwa customers with several criteria in AEON Mall Jakarta Garden City and Transera Waterpark at East Jakarta. There were several criteria used in selecting the sample. First, consumers had purchased Kopi Janji Jiwa in the last six months. Second, they had seen advertisements/promotions of Kopi Janji Jiwa on Instagram. Last, their purchasing decisions were influenced by word of mouth from their friends, family, and others. It obtained 103 respondents. All the responses were processed using path analysis with a smart Partial Least Square (PLS) application. The results reveal that Instagram promotion (advertising), word of mouth, purchase intention, brand awareness, and buying decision influence each other. However, there is no influence between word of mouth on purchase intention. In addition, purchase intention and brand awareness are proven to mediate and strengthen the relationship between Instagram promotion and word of mouth with buying decision. Last, brand awareness is also proven to mediate and strengthen the relationship between word of mouth and purchase intention.
Quality of life of college students: The effects of state anxiety and academic stress with self-control as a mediator
Authors: Nuriyyatiningrum Nadya Ariyani Hasanah , Zikrinawati Khairani , Lestari Puji , Madita Rach
Published: June 2023

College education is expected to equip students with various types of knowledge. In reality, however, the learning process is not always easy. Problems can occur and affect students' quality of life. This study aims to examine the effect of state anxiety and academic stress on the quality of life through the mediation of student self-control. A quantitative method was used involving a sample of 400 students from 24 provinces in Indonesia. The measuring instruments employed were the World Health Organization Quality of Life (WHOQOL), The State Anxiety Scale, the Academic Stress Scale, and the Brief Self-Control Scale. Path analysis was conducted using R software. It was found that self-control partially mediates the effect of state anxiety on the quality of life. State anxiety has a direct effect, with a standardized coefficient of -.351 (p .01), and an indirect one, with a standardized coefficient of -.150 (p .01) on reducing the quality of life through the mediation of self-control. Academic stress has no indirect effect on the quality of life through the mediation of self-control with a standardized coefficient of -.000 (p = .990, p .05). However, it was found to play a direct role in reducing the quality of life, with a standardized coefficient of -.207 (p .01). The findings indicate the multi-dynamic impacts of state anxiety and academic stress toward the quality of life of students. Therefore, the development of anxiety and stress-management skills in students need to be addressed in the higher education management system in order to maintain the quality of life of students.
Digital transformation for the water industry: how a data-driven business intelligence platform can improve operations
Authors: Bettin Alessandro
Published: June 2023

The management of a water network is a complex and challenging job. Nowadays, thanks to the technology available, there is the opportunity to move to a data-driven decision process. To meet the increasingly stringent technical quality requirements from the Authority, water utilities need to increase the operational efficiency. The solution is to integrate and organize operational and business-related data into a common data lake and use these data as a source for a business analytics software able to assist and support daily operations and future investment programs. This paper describes the activities and results of a digitalization case study carried out in Italy where a leading water utility serving over 2 million residents implemented an integrated water management system to enhance the business performance by leveraging a data-driven approach. The project resulted in the establishment of an advanced control room at the company's headquarters, featuring real-time dashboards that are fully integrated with the utility's operational tools and software. The integrated system allows faster responses to operational needs and supports business decisions through streamlined access to relevant data.
Nutritional secondary hyperparathyroidism in a puppy Leopardus geoffroyi (d’Orbigny and Gervais, 1844)
Authors: Sá Mayana Lima , Magnabosco Mariana Wilhelm , Passini Ynara , Bandarra Paulo Mota , Coimbra Marco Antonio Afonso , França Raqueli Teresinha
Published: June 2023

Nutritional Secondary Hyperparathyroidism (NSH) is a metabolic disorder that mainly affects young animals. This disease causes imbalance between the levels of calcium and phosphorus, culminating in bone changes. Wild animals raised in captivity can develop the disease when they are given an inadequate diet. The aim of this study is to report the clinical findings, the complementary exams and the therapeutics utilized in a Leopardus geoffroyi (Geoffroy’s cat) cub originated from captivity. The animal went through complementary imaging exams and biochemical evaluation that respectively revealed generalized osteopenia and pathological fracture of the right femur, reduction of serum calcium level and elevation of serum phosphorus level. After diagnosis, a correction of the diet and mineral and vitamin supplementation was performed. The NSH diagnosis was stablished by associating history, clinical signs, imaging exams findings and serum biochemicals. The early identification of this disease is of paramount importance, because in this way corrections can be made in food management, in order to provide successful clinical recovery and promote quality of life for wild captive animals.
Applicability of management tools in a family dairy farm: a case study
Authors: Fernandes Emanuela Costa , Reis Eduardo Mitke Brandão , Rosa Bruna Laurindo , Monteiro Mariana Benevides , Neves Eduardo Cavalcante das
Published: June 2023

This study aimed to describe the application of the management tool GUT matrix in a family dairy farm in the state of Acre to identify the main weaknesses and propose adequate tools to allow better management of the causes of the three biggest identified problems. This is a case study carried out on a single property located in the municipality of Rio Branco - Acre in May 2022. Information was collected by applying a semi-structured form with 267 questions. The GUT matrix allowed ranking the three main weaknesses of the property, namely: “lack of zootechnical bookkeeping,” “long calving interval,” and “failure in feeding management.” The management tools proposed to the producer, seeking to guide and organize the activities of the property, consisted of 5W2H and Ishikawa diagram.
Campos basin: review on the geology and its oil and natural gas exploration and production context
Authors: Cardoso Vilela Pamela , De Jesus Aline
Published: June 2023

This review and analysis work presents the Campos Basin, in its geological aspects, such as the tectonosedimentary evolution and its petroleum systems and exploratory plays, the history of exploration and production, in post-salt and pre-salt reservoirs, the panorama current production as well as future perspectives for production in the basin, in the context of national medium and long-term forecasts.
Condição sistêmica de pacientes em tratamento periodontal: avaliação de 5 anos
Authors: Claudiane Velozo Alexandre  , Ana Margarida Melo Nunes  , Fernanda Ferreira Lopes  , Adriana de Fátima Vasconcelos Pereira
Published: June 2023

A importância do estudo sobre condições sistêmicas e doença periodontal se dá pelo fato de que há uma relação causal entre elas e por permitir uma melhor conduta clínica aos pacientes. Objetivo: Analisar o perfil sistêmico de pacientes e relacionar com o diagnóstico periodontal. Material e métodos: Este estudo transversal foi realizado com dados secundários de 73 pacientes, com idade mínima de 20 anos, que fizeram tratamento periodontal na Clínica Escola do Curso de Odontologia da Universidade Federal do Maranhão no período de 2014 a 2019. Analisaram-se informações sociodemográficas (idade, gênero), motivo de consulta, recursos e frequência de higiene bucal, hábitos de vida, saúde sistêmica e diagnóstico periodontal. Efetuou-se análise descritiva dos dados expressos para obtenção de números absolutos, relativos e proporções. O teste do qui-quadrado (aderência) foi empregado para verificar a existência de diferença fenotípica entre condição periodontal de diabéticos e de hipertensos, com nível de significância de 5%. Resultados: As condições sistêmicas mais encontradas foram hipertensão arterial (52,1%), seguida de diabete melito (26,0%). Apenas 4,10% dos pacientes atendidos relataram ser fumantes ativos, mantendo o hábito por até 10 anos (66,6%). Com relação à condição periodontal, 60,3% dos pacientes tiveram diagnóstico de periodontite, 32,8% gengivite induzida pelo biofilme dental e 6,8% saúde periodontal e gengival. Conclusão: É possível afirmar que a maioria dos pacientes com diagnóstico de periodontite apresentou hipertensão e/ou diabete melito e não era fumante.
Use of Chemical Reagents in Non-fluorescent Pharmaceutical Labels:
A Comprehensive Review
Authors: Devi Barla Karuna , Madhavi Kuchana , Naraparaju Swathi , Deverakonda Rajeshwari
Published: June 2023

Spectrofluorimetry is an analytical technique with high sensitivity, specificity, and
simplicity, and is very economical. Drugs that lack native fluorescence are derivatized with reagents
so that highly fluorescent compounds are formed that can be readily detected by spectrofluorimetry.
There are different reagents such as 4-chloro-7-nitrobenz-2-oxa-1,3-diazole (NBD-Cl),
N- methylnicotinamide chloride (NMNCl), 9-flurenylmethylchloroformate (FMOC-Cl), dansylchloride
(DNS-Cl), 3-methyl-2-benzothiazolinone hydrazone hydrochloride (MBTH), 1,2-
naphthoquinone-4-sulfonate (NQS), 1,4- benzoquinone, 4-fluro-7-nitrobenzofurazan (NBD-F),
ninhydrin, benzoin, N- bromo succinimide (NBS), acetylacetone & HCHO, ortho-phthalaldehyde
(OPA), and 2-cyanoacetamide used in spectrofluorimetric determination of the drugs that lack native
fluorescence. The present review highlights these reagents with principle, reaction conditions,
and their applications.
Murraya koenigii (Curry Tree): A review of its Phytochemistry, Ethnomedicinal uses, and Pharmacology with Respect to Molecular Mechanisms
Authors: Patil Rutuja , Mandlik Satish , Mandlik Deepa
Published: June 2023

Medicinal plants have significant therapeutic value and are a gift to humanity in pursuing healthy living. The discovery of numerous rejuvenating compounds that can stop or reduce the pathology of many diseases will be a crucial advancement in the coming years. Synthetic compounds can cause health issues and side effects, necessitating the development of molecules derived from plants and other natural resources as viable substitutes for synthetic compounds. Several plant phytochemicals and extracts have been found to have significant effects on traditional medical therapy. Murraya koenigii (M. Koenigii) is a member of the Rutaceae family, well-known in the Ayurvedic system of medicine as a therapeutically important herb of Indian origin. M. Koenigii has been used in several ancient systems of medicine, including Siddha and Unani, as a multi-potential medicinal plant. Previous research has shown that this plant's bark, roots, and leaves are abundant sources of carbazole alkaloids, which have beneficial pharmacological and biological effects. These include antioxidant, antibacterial, antidiabetic, anti-inflammatory, antihypertensive, antifungal, antiprotozoal, hepatoprotective, antihypercholesterolemic, antiulcer, cytotoxic, antidiarrheal, phagocytic, neuroprotective, and antitumor activities. The key components of the M. koenigii plant and their pharmacological activities against various diseases using preclinical models are discussed in this review. Exhaustive studies on the molecular mechanism of action of M. koenigii are needed to validate the effectiveness of curry tree and their constituents as potent therapeutic agents. However, serious efforts are required to identify, isolate and evaluate the chemical components for nutritional and medicinal potentials.
Entrepreneurial aspects of the EU Succession Regulation
Authors: Juric Tomislav
Published: June 2023

This article investigates entrepreneurial aspects of succession law under the EU Succession Regulation. The subject area of entrepreneurial succession takes on a new dimension in a cross-border context. It is now the rule that there is at least one foreign connection within an entrepreneurial family: this may be the case due to a foreign nationality, a foreign habitual residence of the entrepreneur or the existence of company assets abroad, etc. Consequently, the entire process of business succession is strongly influenced by rules of private international law and must be viewed from a new – namely international – standpoint. Each EU Member State has its own conflict of law rules with regard to succession law, which provides an answer to the question of which law should be applied when there is an interference with other legal systems. For the territory of the EU, there has been an EU-wide inheritance law since August 2015 that answers this question – the so – called EU Succession Regulation, which also applies to entrepreneurs in their legal status as natural persons. Despite the fact that company law is explicitly excluded from the scope of application of the EU Succession Regulation, its interfaces with company law are of great practical relevance for the succession of the deceased entrepreneur. The aim of this article is to highlight the various legal aspects of the business succession under the EU Succession Regulation and distinguish them from the rules of private international law.
Revisión taxonómica del género Phylloporia (Hymenochaetaceae, Basidiomycota) en Cuba
Authors: Castro Hernández Lázaro , Camino Vilaró Mayra , Herrera Figueroa Sara
Published: June 2023

Antecedentes y Objetivos: El género Phylloporia es morfológicamente diverso; incluye especies que se desarrollan en áreas restringidas, que son predominantemente tropicales y que poseen o no especificidad por sustratos. Para Cuba se reportan cinco especies; sin embargo, debido a la heterogeneidad de ecosistemas de la Isla y la diversidad del género, se estima que el número de taxones sea superior al registrado. Por ello, el presente trabajo tuvo como objetivo la revisión taxonómica del género Phylloporia en Cuba, a partir del análisis morfológico de ejemplares de herbarios cubanos.
Métodos: Se revisaron especímenes depositados en las colecciones micológicas de los herbarios HAJB, del Jardín Botánico Nacional, y HAC, del Instituto de Ecología y Sistemática de Cuba. Los ejemplares fueron estudiados, descritos y determinados macroscópica y microscópicamente con apoyo de literatura especializada.
Resultados clave: Se determinaron ocho especies del género Phylloporia para Cuba: P. chrysites, P. fruticum, P. pectinata y P. spathulata (previamente registradas); P. verae-crucis (como nuevo registro para el país); P. subpectinata (se propone como nueva combinación); P. pinarensis y P. rodriguezii (como dos especies nuevas para la ciencia). Para estos taxones se ofrecen descripciones de sus estructuras macroscópicas y microscópicas. Además, se presenta un mapa de distribución de las especies tratadas y una clave dicotómica para la identificación de los miembros del género en territorio nacional, en la que se incluye a P. crystallina, que es un taxón no encontrado en este estudio.
Conclusiones: Se amplía el área de distribución geográfica de las especies conocidas del género en Cuba y se incorporan nuevos taxones a la micobiota del país. No obstante, resulta necesario incrementar los muestreos en áreas donde existen vacíos de información o estén poco exploradas, así como en sitios con alto endemismo en plantas. También se precisa implementar análisis moleculares con el objetivo de evaluar las relaciones filogenéticas entre las especies tratadas y otras del género y la familia.
Environmental Land Management and Assessment, in the Context of Resource and Climate-Related Conflict
Authors: Lavers Christopher
Published: June 2023

This paper reviews the growing importance of satellite imagery to provide Normalized Difference Vegetative Index (NDVI) maps, relevant to environmental land management assessment, in the context of resource and climate-related conflict based upon our own humanitarian project-related support. Near real-time space-based monitoring benefits land assessment, human rights observers, and Non-Governmental Organizations (NGOs), in the presence of unstable regimes or socio-economic upheaval. Access to areas to validate claims or allegations with remote sensing tools and digital signal processing techniques is now important. Imagery-based assessment can quantify radiometrically calibrated NDVI, with temporal change indices, may evaluate displacement, to land clearances, and provide metrics on land use change. High-resolution satellite imagery can assess the extent of activities such as open cast mining, and dam construction in inaccessible regions, using semi-automatic orientated methods, generating indices at sub-metric levels derived from satellite data in: Red, Green, Blue, and NIR bands. We discuss the background of space-based applications, and the experimental methodology used. Results and discussion arising from a number of recent cases studies, with specific factors used to help with documentation and formulation of land management risk assessment in the context of climate change threats, e.g. wildfire, are considered. Satellite imagery combined with verified ground data sets of geospatial information and map making, allows external verification of ongoing and planned mining, or construction activities, which impact indigenous communities in such remote geographical regions, for example the controversial Ethiopian Gibe III dam. Seasonal and area change dynamics are observed and discussed.
Towards clearer recognition and easier usefulness: development of a cross-lingual atherosclerotic cerebrovascular disease Ontology (Preprint)
Authors: Ma Hetong , Shen Liu , Wang Jiayang , Li Zixiao , Li Jiao
Published: June 2023

BACKGROUND

Atherosclerotic cerebrovascular disease could result in a great number of deaths and disabilities. However, it did not acquire enough attention. Up till now, less information, statistics, or clear consensus on the disease was revealed. Thus, no systematic concept datasets were released to help clinicians in the field to clarify the scope, assist research, and offer maximized value.

OBJECTIVE

The aims of this study were to (1) develop a comprehensive cross-lingual atherosclerotic cerebrovascular disease ontology. (2) describe the workflow, schema, and hierarchical structure, and the highlighted content of the ontology (3) design a brand-new rehabilitation ontology which was an important part overlooked in the existing ontologies (4) implement the evaluation of the proposed ontology (5) apply the proposed ontology to real-world scenarios and electronic health records to realize information retrieval, named entity recognition, novel expression discovery, and knowledge fusion.

METHODS

We implemented 9 steps based on the ontology development 101 methodologies combined with expert opinions. The final ontology included clinical requirements collection and specification, background investigation and knowledge acquisition, ontology selection and reuse, scope identification, schema definition, concept extraction, concept extension, ontology verification, and ontology evaluation.

RESULTS

The current ontology included 10 top-level classes, respectively clinical manifestation, comorbidity, complication, diagnosis, model of atherosclerotic cerebrovascular disease, pathogenesis, prevention, rehabilitation, risk factor, and treatment. Totally, there are 1715 concepts in the 11-level ontology, covering 4588 Chinese terms, 6617 English terms, and 972 definitions. The ontology could be applied in real-world scenarios such as information retrieval, new expression discovery, named entity recognition, and knowledge fusion, and the use case proved that it could offer satisfying support to related medical scenarios.

CONCLUSIONS

The proposed ontology provided a clear set of cross-lingual concepts and terms with an explicit hierarchical structure, helping scientific researchers to quickly retrieve relevant medical literature, assisting data scientists to efficiently identify relevant contents in electronic health records, and providing a clear domain framework for academic reference.
Psychiatry and Religion: A Stance
Authors: Verhagen Peter J.
Published: June 2023

A dialogue between psychiatry and religion is still not easy to conduct despite the awareness that religion (and spirituality) can play an important role in the way patients cope with their mental disorder. In this contribution the goal is to pave the path in the hope that it will promote dialogue. To achieve this goal, we take three steps. We will start with describing science and religion as social practices characterized by epistemic, practical, collective and individual goals. The next step is some analysis of what we mean by commitment. Based on what we have found we will be able to describe what we mean by a stance, followed by what in our view characterizes mostly the stance “psychiatry and religion”. Holism, values, a certain conception of the professional role, and inclusiveness are the essential issues.
Depression in Schizophrenia
Authors: - Anon
Published: June 2023

None
Redes alimentarias alternativas para la valorización del patrimonio alimentario: propuesta analítica a partir de un estudio de caso
Authors: Martínez Salvador Laura Elena
Published: June 2023

El objetivo de este trabajo es analizar las redes alimentarias alternativas (RAA), materializadas en la organización de productoras y productores ‘Nómadas’, a partir de cinco dimensiones analíticas: 1) identidad de la organización; 2) productos diferenciados y valorización del ‘origen’; 3) rescate de elementos patrimoniales; 4) impulso a la vinculación rural-urbana y a las cadenas cortas de comercialización, y 5) multifuncionalidad del territorio y continuidad de la actividad. Ello, a fin de identificar el potencial de estas RAA para contribuir a la valorización del patrimonio alimentario. El trabajo se realiza a partir de una metodología mixta (cualitativa y cuantitativa) que contrastó las dimensiones anteriormente propuestas con el estudio de caso ‘Nómadas’, el cual fue conformado mediante entrevista semiestructurada, observación no participante y la aplicación de una encuesta a productoras y productores integrantes de la organización. La raa ‘Nómadas’ se posiciona como una red vinculadora de colectivos agroalimentarios cuyo marcado impulso al vínculo alimentos-territorio-cultura ha favorecido la salvaguarda y reproducción del patrimonio alimentario que tiende hacia sistemas agroalimentarios más justos y sostenibles.
México: el costo humano del “exterminio selectivo” y del “exterminio electoral” durante el periodo electoral 2017-2018
Authors: Fracchia Figueiredo Myriam , Ameglio Patella Pietro , Amor Reyes Gabriela
Published: June 2023

En México, el periodo electoral 2017-2018, en nuestras investigaciones acerca del costo humano de los activistas sociales, ha evidenciado que no sólo ellos y los periodistas lo padecieron sino también los candidatos electorales y las autoridades gubernamentales. Presentamos los hallazgos de la investigación exploratoria acerca del exterminio selectivo hacia el conjunto de la referida población, con el fin de visibilizarlo ante la actual espiral de violencia. Para ello, hemos registrado diariamente, de enero de 2017 a diciembre de 2018, las acciones con bajas humanas, mismas que hemos procesado con el Programa de Estadística para Ciencias Sociales (SPSS), desde la perspectiva de que la violencia es inherente al estado del poder en México, resultante del entrelazamiento entre el orden delictual y el orden legal en el proceso del exterminio selectivo, que incluye el del exterminio electoral.