bluuebunny/crossref_metadata_2025 huggingface dataset:
A Consistent Methodology to Evaluate Temperature and Heat Wave Future Projections for Cities: A Case Study for Lisbon
Authors: Rocha Alfredo , Pereira Susana C. , Viceto Carolina , Silva Rui , Neto Jorge , Marta-Almeida Martinho
Published: February 2020

Heat waves are large-scale atmospheric phenomena that may cause heat stress in ecosystems and socio-economic activities. In cities, morbidity and mortality may increase during a heat wave, overloading health and emergency services. In the face of climate change and associated warming, cities need to adapt and mitigate the effects of heat waves. This study suggests a new method to evaluate heat waves’ impacts on cities by considering some aspects of heat waves that are not usually considered in other similar studies. The method devises heat wave quantities that are easy to calculate; it is relevant to assessing their impacts and permits the development of adaptation measures. This study applies the suggested method to quantify various aspects of heat waves in Lisbon for future climate projections considering future mid-term (2046–2065) and long-term (2081–2100) climates under the RCP8.5 greenhouse emission scenario. This is achieved through the analysis of various regional climate simulations performed with the WRF model and an ensemble of EURO-CORDEX models. This allows an estimation of uncertainty and confidence of the projections. To evaluate the climate change properties of heat waves, statistics for future climates are compared to those for a reference recent climate. Simulated temperatures are first bias corrected to minimize the model systematic errors relative to observations. The temperature for mid and long-term futures is expected to increase relative to the present by 1.6 °C and 3.6 °C, respectively, with late summer months registering the highest increases. The number of heat wave days per year will increase on average from 10, in the present climate, to 38 and 63 in mid and long-term climates, respectively. Heat wave duration, intensity, average maximum temperature, and accumulated temperature during a heat wave will also increase. Heat waves account for an annual average of accumulated temperature of 358 °C·day in the present climate, while in the mid and long-term, future climates account for 1270 °C·day and 2078 °C·day, respectively. The largest increases are expected to occur from July to October. Extreme intensity and long-duration heat waves with an average maximum temperature of more than 40 °C are expected to occur in the future climates.
Environmental Attention-Guided Branchy Neural Network for Speech Enhancement
Authors: Zhang Lu , Wang Mingjiang , Zhang Qiquan , Liu Ming
Published: February 2020

The performance of speech enhancement algorithms can be further improved by considering the application scenarios of speech products. In this paper, we propose an attention-based branchy neural network framework by incorporating the prior environmental information for noise reduction. In the whole denoising framework, first, an environment classification network is trained to distinguish the noise type of each noisy speech frame. Guided by this classification network, the denoising network gradually learns respective noise reduction abilities in different branches. Unlike most deep neural network (DNN)-based methods, which learn speech reconstruction capabilities with a common neural structure from all training noises, the proposed branchy model obtains greater performance benefits from the specially trained branches of prior known noise interference types. Experimental results show that the proposed branchy DNN model not only preserved better enhanced speech quality and intelligibility in seen noisy environments, but also obtained good generalization in unseen noisy environments.
Using Fast Frequency Hopping Technique to Improve Reliability of Underwater Communication System
Authors: Schmidt Jan H.
Published: February 2020

Acoustic underwater communication systems designed to work reliably in shallow coastal waters must overcome major limitations such as multipath propagation and the Doppler effect. These restrictions are the reason for the complexity of receivers being built, whose task is to decode a symbol on the basis of the received signal. Additional complications are caused by the low propagation speed of the acoustic wave in the water and the relatively narrow bandwidth. Despite the continuous development of communication systems using coherent modulations, they are still not as reliable as is desirable for reliable data transmission applications. This article presents an acoustic underwater communication system that uses one of the varieties of the spread spectrum technique i.e., the fast frequency hopping technique (FFH). This technique takes advantage of binary frequency-shift keying (BFSK) with an incoherent detection method to ensure the implementation of a system whose main priority is reliable data transmission and secondary priority is the transmission rate. The compromised choice of parameters consisted of the selection between the narrow band of the hydroacoustic transducer and the maximum number of carrier frequency hops, which results from the need to take into account the effects of the Doppler effect. In turn, the number of hops and the symbol duration were selected adequately for the occurrence of multipath propagations of an acoustic wave. In addition, this article describes experimental communication tests carried out using a laboratory model of the FFH-BFSK data transmission system in the shallow water environment of Lake Wdzydze/Poland. The test results obtained for three channels of different lengths are discussed.
Rapid and Nondestructive Discrimination of Geographical Origins of Longjing Tea using Hyperspectral Imaging at Two Spectral Ranges Coupled with Machine Learning Methods
Authors: Hong Zhiqi , He Yong
Published: February 2020

Longjing tea is one of China’s protected geographical indication products with high commercial and nutritional value. The geographical origin of Longjing tea is an important factor influencing its commercial and nutritional value. Hyperspectral imaging systems covering the two spectral ranges of 380–1030 nm and 874–1734 nm were used to identify a single tea leaf of Longjing tea from six geographical origins. Principal component analysis (PCA) was conducted on hyperspectral images to form PCA score images. Differences among samples from different geographical origins were visually observed from the PCA score images. Support vector machine (SVM) and partial least squares discriminant analysis (PLS-DA) models were built using the full spectra at the two spectral ranges. Decent classification performances were obtained at the two spectral ranges, with the overall classification accuracy of the calibration and prediction sets over 84%. Furthermore, prediction maps for geographical origins identification of Longjing tea were obtained by applying the SVM models on the hyperspectral images. The overall results illustrate that hyperspectral imaging at both spectral ranges can be applied to identify the geographical origin of single tea leaves of Longjing tea. This study provides a new, rapid, and non-destructive alternative for Longjing tea geographical origins identification.
Characterization of Wind-Sea- and Swell-Induced Wave Energy along the Norwegian Coast
Authors: Christakos Konstantinos , Varlas George , Cheliotis Ioannis , Spyrou Christos , Aarnes Ole Johan , Furevik Birgitte Rugaard
Published: February 2020

The necessity to reduce C O 2 emissions in combination with the rising energy demand worldwide makes the extensive use of renewable energy sources increasingly important. To that end, countries with long coastlines, such as Norway, can exploit ocean wave energy to produce large amounts of power. In order to facilitate these efforts as well as to provide quantitative data on the wave energy potential of a specific area, it is essential to analyze the weather and climatic conditions detecting any variabilities. The complex physical processes and the atmosphere-wave synergetic effects make the investigation of temporal variability of wave energy a challenging issue. This work aims to shed new light on potential wave energy mapping, presenting a spatio-temporal assessment of swell- and wind-sea-induced energy flux in the Nordic Seas with a focus on the Norwegian coastline using the NORA10 hindcast for the period 1958–2017 (59 years). The results indicate high spatial and seasonal variability of the wave energy flux along the coast. The maximum wave energy flux is observed during winter, while the minimum is observed during summer. The highest coastal wave energy flux is observed in the Norwegian Sea. The majority of areas with dominant swell conditions (i.e., in the Norwegian Sea) are characterized by the highest coastal wave energy flux. The maximum values of wave energy flux in the North Sea are denoted in its northern parts in the intersection with the Norwegian Sea. In contrast to the Norwegian Sea, areas located in the North Sea and the Barents Sea show that wind sea is contributing more than swell to the total wave energy flux.
Molecular Analysis of Membrane Targeting by the C2 Domain of the E3 Ubiquitin Ligase Smurf1
Authors: Scott Jordan L. , Frick Cary T. , Johnson Kristen A. , Liu Haining , Yong Sylvia S. , Varney Allyson G. , Wiest Olaf , Stahelin Robert V.
Published: February 2020

SMAD ubiquitination regulatory factor 1 (Smurf1) is a Nedd4 family E3 ubiquitin ligase that regulates cell motility, polarity and TGFβ signaling. Smurf1 contains an N-terminal protein kinase C conserved 2 (C2) domain that targets cell membranes and is required for interactions with membrane-localized substrates such as RhoA. Here, we investigated the lipid-binding mechanism of Smurf1 C2, revealing a general affinity for anionic membranes in addition to a selective affinity for phosphoinositides (PIPs). We found that Smurf1 C2 localizes not only to the plasma membrane but also to negatively charged intracellular sites, acting as an anionic charge sensor and selective PIP-binding domain. Site-directed mutagenesis combined with docking/molecular dynamics simulations revealed that the Smurf1 C2 domain loop region primarily interacts with PIPs and cell membranes, as opposed to the β-surface cationic patch employed by other C2 domains. By depleting PIPs from the inner leaflet of the plasma membrane, we found that PIP binding is necessary for plasma membrane localization. Finally, we used a Smurf1 cellular ubiquitination assay to show that the amount of ubiquitin at the plasma membrane interface depends on the lipid-binding properties of Smurf1. This study shows the mechanism by which Smurf1 C2 targets membrane-based substrates and reveals a novel interaction for non-calcium-dependent C2 domains and membrane lipids.
Interaction between Sex and LDLR rs688 Polymorphism on Hyperlipidemia among Taiwan Biobank Adult Participants
Authors: Liu Yin-Tso , Nfor Oswald , Wang Lee , Hsu Shu-Yi , Lung Chia-Chi , Tantoh Disline , Wu Min-Chen , Chang Horng-Rong , Liaw Yung-Po
Published: February 2020

Hyperlipidemia is one of the strong risk factors for ischemic heart disease. Using the Taiwan Biobank (TWB) database, we evaluated the risk of hyperlipidemia and its interaction with sex and rs688 polymorphism on the low-density lipoprotein receptor (LDLR) gene. Data collection in the biobank started in 2008 and is ongoing. Data analysis was performed on the participants’ data collected between 2008 and 2015. In general, 27.92% of the 9237 female participants and 32.65% of the 8690 male participants were identified with hyperlipidemia. Compared to the C/C genotype, C/T and T/T genotypes were not significant risk factors for hyperlipidemia (OR = 1.061, CI: 0.976–1.153 for C/T and OR = 1.052, CI: 0.845–1.309 for T/T genotype) in the general model. However, there was a significant interaction between sex and rs6888 on hyperlipidemia risk (p-interaction = 0.0321). With the male sex/CC genotype being the reference group, only the female sex/CT and T/T genotypes were closely associated with hyperlipidemia, with respective ORs of 1.153 (CI: 1.014–1.311) and 1.423 (CI: 1.056–1.917). Our data indicate that rs688 C/T and T/T genotypes may be associated with increased risk of hyperlipidemia in Taiwanese women. These findings may be relevant in lipid-modification therapy.
The Relationship between Executive Functions and Language Production in 5–6-Year-Old Children: Insights from Working Memory and Storytelling
Authors: Veraksa Aleksander , Bukhalenkova Daria , Kartushina Natalia , Oshchepkova Ekaterina
Published: February 2020

This study examined the relationship between working memory capacity and narrative abilities in 5–6-year-old children. 269 children were assessed on their visual and verbal working memory and performed in a story retelling and a story creation (based on a single picture and on a series of pictures) tasks. The stories were evaluated on their macrostructure and microstructure. The results revealed a significant relationship between both components (verbal and visual) of working memory and the global indicators of a story’s macrostructure—such as semantic completeness, semantic adequacy, programming and narrative structure—and with the indicators of a story’s microstructure, such as grammatical accuracy and number of syntagmas. Yet, this relationship was systematically stronger for verbal working memory, as compared to visual working memory, suggesting that a well-developed verbal working memory leads to lexically and grammatically more accurate language production in preschool children.
Religiosity and Psychotic Ideation in Stable Schizophrenia: A Role for Empathic Perspective-Taking
Authors: Duñó Rosó , Oliva Joan Carles , Tobeña Adolf , Palao Diego , Labad Javier
Published: February 2020

The relationship between religiosity and different components of empathy was explored in schizophrenia patients. A total of 81 stable schizophrenia patients and 95 controls from the nearby community completed self-reported questionnaires assessing religiosity and empathy (through the Interpersonal Reactivity Index, IRI). Patients with schizophrenia showed higher religiousness than controls and they presented less perspective-taking and empathic concern but increased personal distress in IRI scores. Regression analyses unveiled an association between religiosity and perspective-taking in schizophrenics after adjusting for age, gender, and psychotic symptoms. In conclusion, religiosity in patients with schizophrenia may be linked to variations in perspective- taking as a component of empathy.
The Impact of Focused Ultrasound in Two Tumor Models: Temporal Alterations in the Natural History on Tumor Microenvironment and Immune Cell Response
Authors: Cohen Gadi , Chandran Parwathy , Lorsung Rebecca M. , Tomlinson Lauren E. , Sundby Maggie , Burks Scott R. , Frank Joseph A.
Published: February 2020

Image-guided focused ultrasound (FUS) has been successfully employed as an ablative treatment for solid malignancies by exposing immune cells to tumor debris/antigens, consequently inducing an immune response within the tumor microenvironment (TME). To date, immunomodulation effects of non-ablative pulsed-FUS (pFUS) on the TME are poorly understood. In this study, the temporal differences of cytokines, chemokines, and trophic factors (CCTFs) and immune cell populations induced by pFUS were interrogated in murine B16 melanoma or 4T1 breast cancer cells subcutaneously inoculated into C57BL/6 or BALB/c mice. Natural history growth characteristics during the course of 11 days showed a progressive increase in size for both tumors, and proteomic analysis revealed a shift toward an immunosuppressive TME. With respect to tumor natural growth, pFUS applied to tumors on days 1, 5, or 9 demonstrated a decrease in the growth rate 24 h post-sonication. Flow cytometry analysis of tumors, LNs, and Sp, as well as CCTF profiles, relative DNA damage, and adaptive T-cell localization within tumors, demonstrated dynamic innate and adaptive immune-modulation following pFUS in early time points of B16 tumors and in advanced 4T1 tumors. These results provide insight into the temporal dynamics in the treatment-associated TME, which could be used to evaluate an immunomodulatory approach in different tumor types.
Non-Coding RNAs as Regulators and Markers for Targeting of Breast Cancer and Cancer Stem Cells
Authors: Prabhu Kirti , Raza Afsheen , Karedath Thasni , Raza Syed , Fathima Hamna , Ahmed Eiman , Kuttikrishnan Shilpa , Therachiyil Lubna , Kulinski Michal , Dermime Said , Junejo Kulsoom , Steinhoff Martin , Uddin Shahab
Published: February 2020

Breast cancer is regarded as a heterogeneous and complicated disease that remains the prime focus in the domain of public health concern. Next-generation sequencing technologies provided a new perspective dimension to non-coding RNAs, which were initially considered to be transcriptional noise or a product generated from erroneous transcription. Even though understanding of biological and molecular functions of noncoding RNA remains enigmatic, researchers have established the pivotal role of these RNAs in governing a plethora of biological phenomena that includes cancer-associated cellular processes such as proliferation, invasion, migration, apoptosis, and stemness. In addition to this, the transmission of microRNAs and long non-coding RNAs was identified as a source of communication to breast cancer cells either locally or systemically. The present review provides in-depth information with an aim at discovering the fundamental potential of non-coding RNAs, by providing knowledge of biogenesis and functional roles of micro RNA and long non-coding RNAs in breast cancer and breast cancer stem cells, as either oncogenic drivers or tumor suppressors. Furthermore, non-coding RNAs and their potential role as diagnostic and therapeutic moieties have also been summarized.
Reply to Comment on “Kadomoto, S. et al. Tumor-Associated Macrophages Induce Migration of Renal Cell Carcinoma Cells via Activation of the CCL20-CCR6 Axis” Cancers 2020 12, 89
Authors: Kadomoto Suguru , Izumi Kouji , Hiratsuka Kaoru , Nakano Taito , Naito Renato , Makino Tomoyuki , Iwamoto Hiroaki , Yaegashi Hiroshi , Shigehara Kazuyoshi , Kadono Yoshifumi , Nakata Hiroki , Saito Yohei , Nakagawa-Goto Kyoko , Mizokami Atsushi
Published: February 2020

We appreciate Zins and Abraham [1] commenting on our paper studying the role of the CCL20-CCR6 axis on renal cell carcinoma (RCC) cells [2]. As they pointed out, our study has certain limitations. Although M1- and M2-types cannot be separated clearly and a consecutive change of character might exist between them, it has been reported that plural specific markers express on M1- and M2-types. Unfortunately, a definite difference between M1 and M2 macrophages was not confirmed in our study. For more differentiation, multiple stimulations, such as suggested in the comments of Zins and Abraham, might be needed. Hence, we needed to expediently use “M1-like” and “M2-like” to mention specific status of these macrophage-like cells. Meanwhile, CCL20 expression levels of M2-like-THP-1 cells co-cultured with RCC cells were dramatically increased compared with parental THP-1 cells, indicating that certain stimulations within the tumor microenvironment rather than theoretical stimulations make macrophages differentiated; however, further studies are needed to clarify this mechanism using a more appropriate co-culture system mimicking the tumor microenvironment. Immunohistochemistry of CCL20 and M2 markers will help to better understand the role of tissue infiltrating macrophages, even tissue CD68 staining intensity itself was reported to correlate with prognosis of RCC patients [3]. [...]
Nickel Phosphide Electrocatalysts for Hydrogen Evolution Reaction
Authors: Hu Cun , Lv Chao , Liu Shuai , Shi Yan , Song Jiangfeng , Zhang Zhi , Cai Jinguang , Watanabe Akira
Published: February 2020

The production of hydrogen through electrochemical water splitting driven by clean energy becomes a sustainable route for utilization of hydrogen energy, while an efficient hydrogen evolution reaction (HER) electrocatalyst is required to achieve a high energy conversion efficiency. Nickel phosphides have been widely explored for electrocatalytic HER due to their unique electronic properties, efficient electrocatalytic performance, and a superior anti-corrosion feature. However, the HER activities of nickel phosphide electrocatalysts are still low for practical applications in electrolyzers, and further studies are necessary. Therefore, at the current stage, a specific comprehensive review is necessary to focus on the progresses of the nickel phosphide electrocatalysts. This review focuses on the developments of preparation approaches of nickel phosphides for HER, including a mechanism of HER, properties of nickel phosphides, and preparation and electrocatalytic HER performances of nickel phosphides. The progresses of the preparation and HER activities of the nickel phosphide electrocatalysts are mainly discussed by classification of the preparation method. The comparative surveys of their HER activities are made in terms of experimental metrics of overpotential at a certain current density and Tafel slope together with the preparation method. The remaining challenges and perspectives of the future development of nickel phosphide electrocatalysts for HER are also proposed.
Sustainable Biodiesel Synthesis from Honne-Rubber-Neem Oil Blend with a Novel Mesoporous Base Catalyst Synthesized from a Mixture of Three Agrowastes
Authors: Falowo Olayomi A. , Ojumu Tunde V. , Pereao Omoniyi , Betiku Eriola
Published: February 2020

Application of solid catalysts synthesized from agricultural wastes provides an environmentally benign and low-cost process path to synthesize biodiesel. An ash containing an equal mixture of cocoa pod husk, plantain peel and kola nut pod husk ashes (CPK) was obtained by open combustion of each of the biomass in air and calcined at 500 °C for 4 h. The calcined CPK ash was characterized to determine its catalytic potential. Two-level transesterification technique was used to synthesize biodiesel using the developed catalyst. The process parameters involved were optimized for the microwave-aided transesterification of a blend of honne, rubber seed and neem oils in a volumetric ratio of 20:20:60, respectively. The study showed that the ash derived from combination of the biomass wastes provided a catalyst which consists all necessary catalytic ingredients in their relative abundance. The calcined CPK consists of 47.67% of potassium, 5.56% calcium and 4.21% magnesium attesting to its heterogenous status. The physisorption isotherms reveals that it was dominantly mesoporous in structure and made up of nanoparticles. A maximum of 98.45 wt.% biodiesel was obtained from a MeOH:oil blend of 12:1, CPK concentration of 1.158 wt.% and reaction time of 6 min under microwave irradiation. The quality of the synthesized biodiesel satisfied the requirements stipulated by standard specifications. Thus, this work demonstrates that a blend of agrowastes and mixtures of non-edible oils could be used to synthesize good quality and sustainable biodiesel that can replace fossil diesel.
Comparing the Nature of Active Sites in Cu-loaded SAPO-34 and SSZ-13 for the Direct Conversion of Methane to Methanol
Authors: Kvande Karoline , Pappas Dimitrios K. , Dyballa Michael , Buono Carlo , Signorile Matteo , Borfecchia Elisa , Lomachenko Kirill A. , Arstad Bjørnar , Bordiga Silvia , Berlier Gloria , Olsbye Unni , Beato Pablo , Svelle Stian
Published: February 2020

On our route towards a more sustainable future, the use of stranded and underutilized natural gas to produce chemicals would be a great aid in mitigating climate change, due to the reduced CO2 emissions in comparison to using petroleum. In this study, we investigate the performance of Cu-exchanged SSZ-13 and SAPO-34 microporous materials in the stepwise, direct conversion of methane to methanol. With the use of X-ray absorption spectroscopy, infrared (in combination with CO adsorption) and Raman spectroscopy, we compared the structure–activity relationships for the two materials. We found that SSZ-13 performed significantly better than SAPO-34 at the standard conditions. From CH4-TPR, it is evident that SAPO-34 requires a higher temperature for CH4 oxidation, and by changing the CH4 loading temperature from 200 to 300 °C, the yield (μmol/g) of SAPO-34 was increased tenfold. As observed from spectroscopy, both three- and four-fold coordinated Cu-species were formed after O2-activation; among them, the active species for methane activation. The Cu speciation in SAPO-34 is distinct from that in SSZ-13. These deviations can be attributed to several factors, including the different framework polarities, and the amount and distribution of ion exchange sites.
A New Highlight of Ephedra alata Decne Properties as Potential Adjuvant in Combination with Cisplatin to Induce Cell Death of 4T1 Breast Cancer Cells In Vitro and In Vivo
Authors: Sioud Fairouz , Amor Souheila , Toumia Imène ben , Lahmar Aida , Aires Virginie , Chekir-Ghedira Leila , Delmas Dominique
Published: February 2020

Despite major advances in the last 10 years, whether in terms of prevention or treatment, the 5 year survival rate remains relatively low for a large number of cancers. These therapeutic failures can be the consequence of several factors associated with the cellular modifications or with the host by itself, especially for some anticancer drugs such as cisplatin, which induces a nephrotoxicity. In the strategy of research for active molecules capable both of exerting a protective action against the deleterious effects of cisplatin and exerting a chemosensitizing action with regard to cancer cells, we tested the potential effects of Ephedra alata Decne extract (E.A.) rich in polyphenolic compounds towards a 4T1 breast cancer model in vitro and in vivo. We showed that E.A. extract inhibited cell viability of 4T1 breast cancer cells and induced apoptosis in a caspase-dependent manner, which involved intrinsic pathways. Very interestingly, we observed a synergic antiproliferative and pro-apoptotic action with cisplatin. These events were associated with a strong decrease of breast tumor growth in mice treated with an E.A./cisplatin combination and simultaneously with a decrease of hepato- and nephrotoxicities of cisplatin.
Silencing Dicer-Like Genes Reduces Virulence and sRNA Generation in Penicillium italicum, the Cause of Citrus Blue Mold
Authors: Yin Chunxiao , Zhu Hong , Jiang Yueming , Shan Yang , Gong Liang
Published: February 2020

The Dicer protein is one of the most important components of RNAi machinery because it regulates the production of small RNAs (sRNAs) in eukaryotes. Here, Dicer1-like gene (Pit-DCL1) and Dicer2-like gene (Pit-DCL2) RNAi transformants were generated via pSilent-1 in Penicillium italicum (Pit), which is the causal agent of citrus blue mold. Neither transformant showed a change in mycelial growth or sporulation ability, but the pathogenicity of the Pit-DCL2 RNAi transformant to citrus fruits was severely impaired, compared to that of the Pit-DCL1 RNAi transformant and the wild type. We further developed a citrus wound-mediated RNAi approach with a double-stranded fragment of Pit-DCL2 generated in vitro, which achieved an efficiency in reducing Pi-Dcl2 expression and virulence that was similar to that of protoplast-mediated RNAi in P. italicum, suggesting that this approach is promising in the exogenous application of dsRNA to control pathogens on the surface of citrus fruits. In addition, sRNA sequencing revealed a total of 69.88 million potential sRNAs and 12 novel microRNA-like small RNAs (milRNAs), four of which have been predicated on target innate immunity or biotic stress-related genes in Valencia orange. These data suggest that both the Pit-DCL1 and Pit-DCL2 RNAi transformants severely disrupted the biogenesis of the potential milRNAs, which was further confirmed for some milRNAs by qRT-PCR or Northern blot analysis. These data suggest the sRNAs in P. italicum that may be involved in a molecular virulence mechanism termed cross-kingdom RNAi (ck-RNAi) by trafficking sRNA from P. italicum to citrus fruits.
Involvement of TRPC4 and 5 Channels in Persistent Firing in Hippocampal CA1 Pyramidal Cells
Authors: Arboit Alberto , Reboreda Antonio , Yoshida Motoharu
Published: February 2020

Persistent neural activity has been observed in vivo during working memory tasks, and supports short-term (up to tens of seconds) retention of information. While synaptic and intrinsic cellular mechanisms of persistent firing have been proposed, underlying cellular mechanisms are not yet fully understood. In vitro experiments have shown that individual neurons in the hippocampus and other working memory related areas support persistent firing through intrinsic cellular mechanisms that involve the transient receptor potential canonical (TRPC) channels. Recent behavioral studies demonstrating the involvement of TRPC channels on working memory make the hypothesis that TRPC driven persistent firing supports working memory a very attractive one. However, this view has been challenged by recent findings that persistent firing in vitro is unchanged in TRPC knock out (KO) mice. To assess the involvement of TRPC channels further, we tested novel and highly specific TRPC channel blockers in cholinergically induced persistent firing in mice CA1 pyramidal cells for the first time. The application of the TRPC4 blocker ML204, TRPC5 blocker clemizole hydrochloride, and TRPC4 and 5 blocker Pico145, all significantly inhibited persistent firing. In addition, intracellular application of TRPC4 and TRPC5 antibodies significantly reduced persistent firing. Taken together these results indicate that TRPC4 and 5 channels support persistent firing in CA1 pyramidal neurons. Finally, we discuss possible scenarios causing these controversial observations on the role of TRPC channels in persistent firing.
BGN/TLR4/NF-κB Mediates Epigenetic Silencing of Immunosuppressive Siglec Ligands in Colon Cancer Cells
Authors: Huang Hsiang-Chi , Cai Bi-He , Suen Ching-Shu , Lee Hsueh-Yi , Hwang Ming-Jing , Liu Fu-Tong , Kannagi Reiji
Published: February 2020

Human Toll-like receptor (TLR) signaling plays a vital role in intestinal inflammation by activating the NF-κB pathway. By querying GENT2 datasets, we identified the gene expression level of TLR2 and TLR4 as being substantially increased in colorectal cancer. Introduction of shRNAs for TLR4 but not TLR2 dramatically recovered disialyl Lewisa and sialyl 6-sulfo Lewisx glycans, which are preferentially expressed in non-malignant colonic epithelial cells and could serve as ligands for the immunosuppressive molecule Siglec-7. We screened several TLR4 ligands and found that among them BGN is highly expressed in cancers and is involved in the epigenetic silencing of Siglec-7 ligands. Suppression of BGN expression substantially downregulated NF-κB activity and the marker H3K27me3 in the promoter regions of the SLC26A2 and ST6GalNAc6 genes, which are involved in the synthesis of those glycans, and restored expression of normal glycans as well as Siglec-7 binding activities. We show that in the presence of TLR4, inflammatory stimuli initiate a positive loop involving NF-κB that activates BGN and further enhances TLR4 activity. Present findings indicate a putative mechanism for the promotion of carcinogenesis by loss of immunosuppressive ligands by the BGN/TLR4/ NF-κB pathway.
Influence of Graphene and Graphene Oxide on Properties of Spark Plasma Sintered Si3N4 Ceramic Matrix
Authors: Balazsi Katalin , Furkó Mónika , Klimczyk Piotr , Balázsi Csaba
Published: February 2020

The sintering of ceramic matrix composites is usually carried out by raising the sintering temperature below the melting point of components. Spark plasma sintering (SPS) has the capability to densify ceramics at a relatively low temperature in a very short time. Two different additions, multilayered graphene (MLG) and graphene oxide (GrO), were added to Si3N4 ceramic matrix in various amount; 5 wt% and 30 wt%. The influence of reinforcing phase on final properties of spark plasma sintered Si3N4 composite was studied. The uniaxial-pressure-assisted SPS sintering resulted in a preferential alignment of both type of graphene in the Si3N4 ceramic matrix, leading to highly anisotropic properties with lower mechanical behavior but better tribological and electrical properties.
Relative Ti2AlC Scale Volatility under 1300 °C Combustion Conditions
Authors: L. Smialek James
Published: February 2020

Turbine environments may degrade high temperature ceramics because of volatile hydroxide reaction products formed in water vapor. Accordingly, the volatility of transient TiO2 and steady-state Al2O3 scales formed on the oxidation-resistant Ti2AlC MAX phase ceramic was examined in 1300 °C high velocity (Mach 0.3, 100 m/s) and high pressure (6 atm, 25 m/s) burner rig tests (BRT). Unlike metals, the ceramic was stable at 1300 °C. Unlike SiC and Si3N4, neither burner test produced a weight loss, unless heavily pre-oxidized. Lower mass gains were produced in the BRT compared to furnace tests. The commonly observed initial, fast TiO2 transient scale was preferentially removed in hot burner gas (~10% water vapor). A lesser degree of gradual Al2O3 volatilization occurred, indicated by grain boundary porosity and crystallographic etching. Modified cubic-linear (growth-volatility) kinetics are suggested. Gas velocity and water vapor pressure play specific roles for each scale. Furthermore, a 7YSZ TBC on Ti2AlC survived for 500 h in the Mach 0.3 burner test at 1300 °C with no indication of volatility or spalling.
An Integrated Method for Multi-Objective Optimization of Multi-Pass Fe50/TiC Laser Cladding on AISI 1045 Steel based on Grey Relational Analysis and Principal Component Analysis
Authors: Wang Qianting , Zeng Xianbin , Chen Changrong , Lian Guofu , Huang Xu
Published: February 2020

As an essentially multi-input multi-output process, determination of optimal conditions for laser cladding normally requires multi-objective optimization. To understand multi-response coupling, the effects of processing parameters on the morphology quality of multi-pass laser claddings of Fe50/TiC on medium carbon steel AISI 1045 were investigated based on composite central design using response surface methodology. Multiple responses, including clad width, flatness, and non-fusion area, were transformed into a single objective through grey relational analysis, with weights objectively identified by principal component analysis. The correlation between grey relational grade (GRG) and process parameters was established by regression analysis. The results show that the GRG response model has excellent goodness of fit and predictive performance. A validation experiment was conducted at the process condition optimized for maximum GRG. The relative error of the predicted optimal GRG is 4.87% whereas those of interested individual objectives, i.e. clad width, flatness, and non-fusion area, are 5.73%, 2.97%, and 6.73%, respectively, which verifies the accuracy of the established model. The investigation of mechanical properties suggests the hardness of substrate can be improved from 20 HRC to 60 HRC and wear resistance to over 8.14 times better.
Effects of Double Diffusion Convection on Third Grade Nanofluid through a Curved Compliant Peristaltic Channel
Authors: Alolaiyan Hanan , Riaz Arshad , Razaq Abdul , Saleem Neelam , Zeeshan Ahmed , Bhatti Muhammad Mubashir
Published: February 2020

Nanofluids are potential heat transfer fluids with improved thermophysical properties and heat transfer performance. Double diffusion convection plays an important role in natural processes and technical applications. The effect of double convection by diffusion is not limited to oceanography, but is also evident in geology, astrophysics, and metallurgy. For such a vital role of such factors in applications, the authors have presented the analytical solutions of pumping flow of third-grade nanofluid and described the effects of double diffusion convection through a compliant curved channel. The model used for the third-grade nanofluid includes the presence of Brownian motion and thermophoresis. Additionally, thermal energy expressions suggest regular diffusion and cross-diffusion terms. The governing equations have been constructed for incompressible laminar flow of the non-Newtonian nanofluid along with the assumption of long wavelength. The obtained analytical expressions for velocity, temperature, and nanoparticle concentration have been sketched for various considerable parameters. The effects of regular buoyancy ratio, buoyancy parameter, modified Dufour parameter, and Dufour-solutal Lewis number have been analyzed along with wall properties and pumping characteristics. This study concludes that fluid becomes hotter with increase in regular buoyancy ratio and a modified Dufour parameter, but a decrease in temperature is observed for the buoyancy parameter. Moreover, the solutal concentration is behaving inversely against the Defour-Solutal Lewis number.
Electrical and Reliability Characteristics of Self-Forming Barrier for CuNd/SiOCH Films in Cu Interconnects
Authors: Cheng Yi-Lung , Lee Chih-Yen , Peng Wei-Fan , Chen Giin-Shan , Fang Jau-Shiung
Published: February 2020

In this study, Cu-2.2 at. % Nd alloy films using a co-sputtering deposition method were directly deposited onto porous low-dielectric-constant (low-k) films (SiOCH). The effects of CuNd alloy film on the electrical properties and reliability of porous low-k dielectric films were studied. The electrical characteristics and reliability of the porous low-k dielectric film with CuNd alloy film were enhanced by annealing at 425 °C. The formation of self-forming barrier at the CuNd/SiOCH interface was responsible for this improvement. Therefore, integration with CuNd and porous low-k dielectric is a promising process for advanced Cu interconnects.
Evaluation of the Antibacterial Activity of a Geopolymer Mortar Based on Metakaolin Supplemented with TiO2 and CuO Particles Using Glass Waste as Fine Aggregate
Authors: Gutiérrez Ruby , Villaquirán-Caicedo Mónica , Ramírez-Benavides Sandra , Astudillo Myriam , Mejía Daniel
Published: February 2020

Metakaolin-based geopolymer cements were produced by alkaline activation with a potassium hydroxide and potassium silicate solution. To produce the geopolymer composites, 10 wt.% titanium oxide (TiO2) and 5 wt.% copper oxide (CuO) nanoparticles were used. The geopolymer mortar was prepared using glass waste as fine aggregate. The raw materials and materials produced were characterized by X-ray diffraction, electron microscopy, and Fourier-transform infrared spectroscopy techniques. Likewise, the geopolymer samples were characterized to determine their physical properties, including their density, porosity, and absorption. The photocatalytic activity of the materials was evaluated by activating the nanoparticles in a chamber with UV–Vis light during 24 h; then, different tests were performed to determine the growth inhibition of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa bacteria in nutrient agar for times of up to 24 h. The study results showed that a geopolymer mortar containing glass waste as fine aggregate (GP-G) exhibited a water absorption 56.73% lower than that of the reference geopolymer paste without glass (GP). Likewise, glass particles allowed the material to have a smoother and more homogeneous surface. The pore volume and density of the GP-G were 37.97% lower and 40.36% higher, respectively, than those of the GP. The study with bacteria showed that, after 24 h in the culture media, the GP-G mortars exhibited a high inhibition capacity for the growth of P. aeruginosa from solutions of 10−4 mL and in solutions of 10−6 mL for E. coli and S. aureus. These results indicate the possibility of generating antibacterial surfaces by applying geopolymer composite.
Double Palindrome Water Chain in Cu(II) Theophylline Complex. Synthesis, Characterization, Biological Activity of Cu(II), Zn(II) Complexes with Theophylline
Authors: Gacki Michał , Kafarska Karolina , Pietrzak Anna , Korona-Głowniak Izabela , Wolf Wojciech M.
Published: February 2020

Two metal complexes of theophylline were synthesized. Namely, 1 with the formula [Cu(theop)2(H2O)3]·2H2O and 2, [Zn(theop)2]∙H2O (where: theop = theophylline ion). Their properties were thoroughly investigated by the elemental analysis (EA), flame atomic absorption spectrometry (FAAS), Fourier-transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA) that were augmented by antimicrobial and antioxidant analyses. Their radical scavenging ability (RSA) is notably higher than that of a pure theophylline itself. Similarly to theophylline complexes already studied by us 3, [Mn(theop)2(H2O)4] 4, [Co(theop)2(H2O)4] and 5, [Ni(theop)2(H2O)4] title compounds are inactive against Gram-negative bacteria, but they show moderate or mild activity against Gram-positive rods. The low temperature, single crystal X-ray diffraction technique determines the crystal structure of 1. Its supramolecular crystal topology is affected by the unique, double palindrome water chain that formed by two conserved and a sole coordinated water molecules. Crystal packing arrangements were characterized by fingerprint plots that were derived from the Hirshfeld surfaces (HS), as calculated for all structures in the series 1, 3, 4, 5.
Correlation between Crystal Structure and Thermoelectric Properties of Sr1−xTi0.9Nb0.1O3−δ Ceramics
Authors: Prado-Gonjal J. , López C. , Pinacca R. , Serrano-Sánchez F. , Nemes N. , Dura O. , Martínez J.L. , Fernández-Díaz M.T. , Alonso J.A.
Published: February 2020

Polycrystalline Sr1−xTi0.9Nb0.1O3−δ (x = 0, 0.1, 0.2) ceramics have been prepared by the solid state method and their structural and thermoelectric properties have been studied by neutron powder diffraction (NPD), thermal, and transport measurements. The structural analysis of Sr1-xTi0.9Nb0.1O3−δ (x = 0.1, 0.2) confirms the presence of a significant amount of oxygen vacancies, associated with the Sr-deficiency of the materials. The analysis of the anisotropic displacement parameters (ADPs) indicates a strong softening of the overall phonon modes for these samples, which is confirmed by the extremely low thermal conductivity value (κ ≈ 1.6 W m-1 K−1 at 823 K) found for Sr1−xTi0.9Nb0.1O3−δ (x = 0.1, 0.2). This approach of introducing A-site cation vacancies for decreasing the thermal conductivity seems more effective than the classical substitution of strontium by rare-earth elements in SrTiO3 and opens a new optimization scheme for the thermoelectric properties of oxides.
The Relationship between Coronary Artery Wall Shear Strain and Plaque Morphology: A Systematic Review and Meta-Analysis
Authors: Bajraktari Artan , Bytyçi Ibadete , Henein Michael Y.
Published: February 2020

Background and Aim: Arterial wall shear strain (WSS) has been proposed to impact the features of atherosclerotic plaques. The aim of this meta-analysis was to assess the impact of different types of WSS on plaque features in coronary artery disease (CAD). Methods: We systematically searched PubMed-Medline, EMBASE, Scopus, Google Scholar, and the Cochrane Central Registry, from 1989 up to January 2020 and selected clinical trials and observational studies which assessed the relationship between WSS, measured by intravascular ultrasound (IVUS), and plaque morphology in patients with CAD. Results: In four studies, a total of 72 patients with 13,098 coronary artery segments were recruited, with mean age 57.5 ± 9.5 years. The pooled analysis showed that low WSS was associated with larger baseline lumen area (WMD 2.55 [1.34 to 3.76, p < 0.001]), smaller plaque area (WMD −1.16 [−1.84 to −0.49, p = 0.0007]), lower plaque burden (WMD −12.7 [−21.4 to −4.01, p = 0.04]), and lower necrotic core area (WMD −0.32 [−0.78 to 0.14, p = 0.04]). Low WSS also had smaller fibrous area (WMD −0.79 [−1.88 to 0.30, p = 0.02]) and smaller fibro-fatty area (WMD −0.22 [−0.57 to 0.13, p = 0.02]), compared with high WSS, but the dense calcium score was similar between the two groups (WMD −0.17 [−0.47 to 0.13, p = 0.26]). No differences were found between intermediate and high WSS. Conclusions: High WSS is associated with signs of plaque instability such as higher necrotic core, higher calcium score, and higher plaque burden compared with low WSS. These findings highlight the role of IVUS in assessing plaque vulnerability.
Biomedical Insights That Inform the Diagnosis of ME/CFS
Authors: Lidbury Brett A. , Fisher Paul R.
Published: February 2020

It is well known that myalgic encephalomyelitis (ME) and chronic fatigue syndrome (CFS), whether considered as separate diseases or as the one chronic syndrome, continue to generate debate [...]
Design and Implementation of a Digital Dual Orthogonal Outputs Chaotic Oscillator
Authors: Berviller Yves , Tisserand Etienne , Poure Philippe , Rabah Hassan
Published: February 2020

Discrete time dynamical chaotic systems obey a set of recurrence equations involving one or more variables. Many chaotic maps have been proposed. None that simultaneously provides two sine–cosine outputs has stationary mean and standard deviation, or is quite robust with respect to the data format used in the hardware implementation. Here, we propose a chaotic oscillator based on a complex phasor whose angular argument evolves according to a geometric progression that is independent of the instantaneous amplitude. In order to maintain the oscillations, the phasor magnitude is normalized at each iteration using an approximation factor. The statistical characteristics of this oscillator are stationary in the short term, and do not depend on the initial conditions. The mean and standard deviation of the two orthogonal sequences quickly approach 0 and 1 / 2 , respectively. The resulting distribution is similar to that of a digital sine with a constant angular step. We also present an FPGA architecture and its implementation results. This oscillator can be used in modulation schemes, such as the chaotic shift keying one or for data and image encryption. Finally, we show an original application that exploits the orthogonality of the two chaotic signals for the simultaneous encryption of two digital images.
Four-Features Evaluation of Text to Speech Systems for Three Social Robots
Authors: Alonso Martin Fernando , Malfaz María , Castro-González Álvaro , Castillo José Carlos , Salichs Miguel Ángel
Published: February 2020

The success of social robotics is directly linked to their ability of interacting with people. Humans possess verbal and non-verbal communication skills, and, therefore, both are essential for social robots to get a natural human–robot interaction. This work focuses on the first of them since the majority of social robots implement an interaction system endowed with verbal capacities. In order to do this implementation, we must equip social robots with an artificial voice system. In robotics, a Text to Speech (TTS) system is the most common speech synthesizer technique. The performance of a speech synthesizer is mainly evaluated by its similarity to the human voice in relation to its intelligibility and expressiveness. In this paper, we present a comparative study of eight off-the-shelf TTS systems used in social robots. In order to carry out the study, 125 participants evaluated the performance of the following TTS systems: Google, Microsoft, Ivona, Loquendo, Espeak, Pico, AT&T, and Nuance. The evaluation was performed after observing videos where a social robot communicates verbally using one TTS system. The participants completed a questionnaire to rate each TTS system in relation to four features: intelligibility, expressiveness, artificiality, and suitability. In this study, four research questions were posed to determine whether it is possible to present a ranking of TTS systems in relation to each evaluated feature, or, on the contrary, there are no significant differences between them. Our study shows that participants found differences between the TTS systems evaluated in terms of intelligibility, expressiveness, and artificiality. The experiments also indicated that there was a relationship between the physical appearance of the robots (embodiment) and the suitability of TTS systems.