bluuebunny/crossref_metadata_2025 huggingface dataset:
Profiling and assessing the risks of image- and performance-enhancing drugs use during the COVID-19 lockdown
Authors: De Luca Ilaria , Di Carlo Francesco , Burkauskas Julius , Dores Artemisa R. , Carvalho Irene P. , Gómez-Martínez M. Ángeles , Szabo Attila , Fujiwara Hironobu , Barbosa Cristina Monteiro , Di Nicola Marco , Mazza Marianna , Sani Gabriele , Luciani Debora , Pettorruso Mauro , di Giannantonio Massimo , Cataldo Ilaria , Esposito Gianluca , Martinotti Giovanni , Zandonai Thomas , Rabin Olivier , Corazza Ornella
Published: June 2024

Background

Image and Performance-Enhancing Drugs (IPEDs) can enhance mental and physical capabilities and impact one’s overall health. Initially confined in sport environments, IPEDs use has become increasingly widespread in a high-performing society. The present study was aimed at profiling IPEDs use during the COVID-19 lockdown among an international sample of young adults.

Methods

A cross-sectional observational study was carried out in eight countries (United Kingdom, Italy, Lithuania, Hungary, Portugal, Spain, Brazil, and Japan) between April and May 2020. The survey questionnaire included validated measurements such as Exercise Addiction Inventory (EAI), Appearance Anxiety Inventory (AAI), and Self-Compassion Scale (SCS) as well as questions about the type of IPEDs, purchasing methods and socio-demographic information.

Results

A total of 736 IPEDs users were included in the survey. Their mean age was 33.05 years (±SD = 10.06), and 64.2% were female participants. Overall, 6.8% were found at risk of exercise addiction (EAI >24), 27.6% presented high levels of appearance anxiety, and 24.9% revealed low levels of emotional regulation’s self-compassion. Most participants (55.6%) purchased IPEDs through pharmacies/specialized shops, while 41.3% purchased IPEDs on the Internet. Online IPEDs buyers were mainly men who had higher scores on the Exercise Addiction Inventory. One or more IPEDs classifiable as “potentially risky” were used by 66.3% of the sample. Users of “potentially risky IPEDs” were younger and primarily men. They showed higher scores both on the Exercise Addiction Inventory and Appearance Anxiety Inventory.

Conclusion

This study profiled users of IPEDs when the most restrictive COVID-19 lockdown policies were implemented in all the participating countries. More targeted post-COVID 19 prevention strategies should be implemented according to the emerged socio-demographic and psychopathological traits and cross-cultural differences emerged. Longitudinal studies will also be needed to determine the long-term effect of the COVID-19 lockdown on IPEDs consumption.
A review of evidence on mechanical properties of running specific prostheses and their relationship with running performance
Authors: Rahnama Leila , Soulis Kimberly , Geil Mark D.
Published: June 2024

Background

Although mechanical properties of running specific prostheses (RSPs) can affect running performance, manufacturers do not consistently report them. This study aimed to review existing literature on RSP mechanical and structural properties and their relationship with running performance.

Methods

A comprehensive search was conducted using keywords related to mechanical properties of RSPs and running performance. Search terms included stiffness and hysteresis, as well as performance outcomes including metabolic cost and running speed. Non-peer-reviewed and non-English publications were excluded.

Results

Twenty articles were included in the review. Sixteen studies used a material testing machine to measure RSP material properties, and four articles used other techniques including 2D/3D video capture and force platforms. Both measurement techniques and reporting of outcomes were inconsistent, which limits the ability to draw broad conclusions. Additionally, several studies did not report the numerical data for material properties despite measuring them. Relatively few articles measured both material properties and running performance and assessed correlations.

Conclusion

Several articles connected prosthesis properties to running performance. However, inconsistent measurement and reporting of mechanical properties, along with the multifactorial nature of the athlete-prosthesis system, limit the ability to draw broad conclusions regarding the relationship between material and structural properties and athlete performance. Current evidence may be useful for clinicians seeking ways to optimize RSP stiffness in a case-by-case basis; however, clinicians would benefit from more consistent and systematic comparisons of the attributes of different RSPs and their role in performance.
How the experiences of implementation support recipients contribute to implementation outcomes
Authors: Metz Allison J. , Jensen Todd M. , Afkinich Jenny L. , Disbennett Mackensie E. , Farley Amanda B.
Published: June 2024

Introduction

There is a growing body of literature on the activities and competencies of implementation support practitioners (ISPs) and the outcomes of engaging ISPs to support implementation efforts. However, there remains limited knowledge about the experiences of implementation support recipients who engage with ISPs and how these experiences shape the trajectory of implementation and contribute to implementation outcomes. This study aimed to extend the research on ISPs by describing the experiences of professionals who received implementation support and inform our understanding of the mechanisms by which ISPs produce behavior change and contribute to implementation outcomes.

Methods

Thirteen individuals with roles in supporting implementation efforts at a private foundation participated in semi-structured interviews. Data were analyzed using qualitative narrative analysis and episode profile analysis approaches. Iterative diagramming was used to visualize the pathway of experiences of implementation support recipients evidenced by the interview data.

Results

The majority of recipients described how positive experiences and trusting relationships with ISPs increased acceptance of implementation science throughout the foundation and increased the perception of implementation science as both an appropriate and feasible approach for strengthening the impact of foundation strategies. As perceptions of appropriateness and feasibility increased, recipients of implementation support described increasing knowledge and application of implementation science in their funding engagements and internal foundation strategies. Finally, recipients reported that the application of implementation science across the foundation led to sustained implementation capacity and better outcomes.

Discussion

The experiences of implementation support recipients described in this paper provide a source for further understanding the mechanisms of change for delivering effective implementation support leading to better implementation quality. Insights from these experiences can enhance our understanding for building implementation capacity and the rationales for evolving approaches that emphasize the dynamic, emotional, and highly relational nature of supporting others to use evidence in practice.
Evaluating pedestrian facilities for enhancing pedestrian safety in Addis Ababa city
Authors: Walelign Bishaw Temesgen , Nurye Dolebo Gebrechiristos , Singh Ranavijai Bahadur
Published: June 2024

Transport demand has been increasing in most third-world countries. However, poorly maintained facilities and poor planning exposed pedestrians to high-traffic accidents. This paper aims to evaluate pedestrian facilities using the pedestrian level of service to enhance pedestrian safety in Addis Ababa. Relevant data was collected through field measurement and direct observation from 13 purposively identified pedestrian sites across the Piyasa to Bole bridge and Megenagna to Torihayloch road corridors. Pedestrian level of service derivation considered capacity, traffic, comfort, safety, and security conditions and their sub-components. The weight of each criterion and sub-factor was decided according to the Saaty scale using the judgment of experts under the analytical hierarchical process. The result of the study strongly stipulates the presence of the problem of pedestrian facilities in Megenagna, Mexico, Urael Church, and Golagol which can affect the level of pedestrian safety. However, Denibel City, Legahare, Torhayloch, and National Theater sites have scored better Pedestrian level of service for the walking population. Finally, the study recommended the requirement of prior attention for the sites with very poor and poor pedestrian levels of service by the concerned sectors.
Rosa roxburghii tratt residue as an alternative feed for improving growth, blood metabolites, rumen fermentation, and slaughter performance in Hu sheep
Authors: Li Huijie , Song Xinyu , Wu Wenxuan , Zhou Chuanshe
Published: June 2024

The utilization of agro-industrial by-products, such as fruit residues, presents a promising strategy for providing alternative feed to ruminants amidst rising prices and limited availability of traditional roughage. In this study, we investigated the effects of Rosa roxburghii tratt residue, a local fruit residue in Guizhou province of China, on the growth, blood metabolites, rumen fermentation, and slaughter performance of Hu sheep. Ninety-six sheep were randomly divided into four groups, namely control, treatment 1, treatment 2, and treatment 3, and fed diets containing 0, 10, 20, and 30% Rosa roxburghii Tratt residue, respectively. Feeding varying levels of Rosa roxburghii Tratt residue showed no significant differences in dry matter intake, average daily gain, or the ratio of dry matter intake to average daily gain. However, sheep in the group fed with 30% Rosa roxburghii Tratt residue showed the highest gross profit. Plasma albumin content was lower in groups fed with Rosa roxburghii Tratt residue-containing diets compared to the control group (p < 0.05). Additionally, diet treatment 3 decreased plasma creatinine levels compared to control and treatment 1 (p < 0.05). Sheep in treatment 2 and treatment 3 exhibited higher plasma high-density lipoprotein level than control and treatment 1 (p < 0.05), as well as increased total cholesterol levels compared to control (p < 0.05). There were no significant differences in other plasma metabolites. Rumen pH, N-NH3, volatile fatty acids, and methane levels did not differ significantly among the four groups. However, feeding diets treatment 2 and treatment 3 resulted in decreased water holding capacity and increased shear force compared to control and treatment 1 (p < 0.05). Furthermore, pH, red chromaticity (a*), yellowness index (b*), and luminance (L*) were unaffected among the four groups of sheep. In conclusion, the inclusion of up to 30% Rosa roxburghii Tratt residue had no adverse effects on growth performance, allowing for feed cost savings without impacting rumen fermentation parameters. Rosa roxburghii tratt residue also showed benefits in improving plasma protein efficiency and enhancing lipid metabolism, albeit with limited effects on meat quality. Considering its affordability, Rosa roxburghii Tratt residue presents a practical choice for low-cost diets, ensuring economic returns.
Does “Dr. Google” improve discussion and decisions in small animal practice? Dog and cat owners use of internet resources to find medical information about their pets in three European countries
Authors: Springer Svenja , Lund Thomas Bøker , Corr Sandra A. , Sandøe Peter
Published: June 2024

Modern dog and cat owners increasingly use internet resources to obtain information on pet health issues. While access to online information can improve owners’ knowledge of patient care and inform conversations with their veterinarian during consultations, there is also a risk that owners will misinterpret online information or gain a false impression of current standards in veterinary medicine. This in turn can cause problems or tensions, for example if the owner delays consulting their veterinarian about necessary treatment, or questions the veterinarian’s medical advice. Based on an online questionnaire aimed at dog and cat owners in Austria, Denmark and the United Kingdom (N = 2117) we investigated the use of internet resources to find veterinary medical information, the type of internet resources that were used, and whether owner beliefs explain how often they used the internet to find medical information about their pet. Approximately one in three owners reported that they never used internet resources prior to (31.7%) or after (37.0%) a consultation with their veterinarian. However, when owners do make use of the internet, our results show that they were more likely to use it before than after the consultation. The most common internet resources used by owners were practice websites (35.0%), veterinary association websites (24.0%), or ‘other’ websites providing veterinary information (55.2%). Owners who believe that the use of internet resources enables them to have a more informed discussion with their veterinarians more often use internet resources prior to a consultation, whereas owners who believed that internet resources help them to make the right decision for their animal more often use internet resources after a consultation. The results suggest that veterinarians should actively ask pet owners if they use internet resources, and what resources they use, in order to facilitate open discussion about information obtained from the internet. Given that more than a third of pet owners use practice websites, the findings also suggest that veterinarians should actively curate their own websites where they can post information that they consider accurate and trustworthy.
Prevention and Rehabilitation After Heart Transplantation: A Clinical Consensus Statement of the European Association of Preventive Cardiology, Heart Failure Association of the ESC, and the European Cardio Thoracic Transplant Association, a Section of ESOT
Authors: Simonenko Maria , Hansen Dominique , Niebauer Josef , Volterrani Maurizio , Adamopoulos Stamatis , Amarelli Cristiano , Ambrosetti Marco , Anker Stefan D. , Bayes-Genis Antonio , Ben Gal Tuvia , Bowen T. Scott , Cacciatore Francesco , Caminiti Giuseppe , Cavarretta Elena , Chioncel Ovidiu , Coats Andrew J. S. , Cohen-Solal Alain , D’Ascenzi Flavio , de Pablo Zarzosa Carmen , Gevaert Andreas B. , Gustafsson Finn , Kemps Hareld , Hill Loreena , Jaarsma Tiny , Jankowska Ewa , Joyce Emer , Krankel Nicolle , Lainscak Mitja , Lund Lars H. , Moura Brenda , Nytrøen Kari , Osto Elena , Piepoli Massimo , Potena Luciano , Rakisheva Amina , Rosano Giuseppe , Savarese Gianluigi , Seferovic Petar M. , Thompson David R. , Thum Thomas , Van Craenenbroeck Emeline M.
Published: June 2024

Little is known either about either physical activity patterns, or other lifestyle-related prevention measures in heart transplantation (HTx) recipients. The history of HTx started more than 50 years ago but there are still no guidelines or position papers highlighting the features of prevention and rehabilitation after HTx. The aims of this scientific statement are (i) to explain the importance of prevention and rehabilitation after HTx, and (ii) to promote the factors (modifiable/non-modifiable) that should be addressed after HTx to improve patients’ physical capacity, quality of life and survival. All HTx team members have their role to play in the care of these patients and multidisciplinary prevention and rehabilitation programmes designed for transplant recipients. HTx recipients are clearly not healthy disease-free subjects yet they also significantly differ from heart failure patients or those who are supported with mechanical circulatory support. Therefore, prevention and rehabilitation after HTx both need to be specifically tailored to this patient population and be multidisciplinary in nature. Prevention and rehabilitation programmes should be initiated early after HTx and continued during the entire post-transplant journey. This clinical consensus statement focuses on the importance and the characteristics of prevention and rehabilitation designed for HTx recipients.
Mechanical Properties and Energy Damage Evolution Mechanism of Basalt Fiber-Modified Tailing Sand Cementation and Filling Body Mechanics
Authors: Chen Rongsen , Zhang Yaoping
Published: June 2024

In order to investigate the mechanical properties of basalt fiber-doped tailing sand cemented filler and the evolution of energy damage, a uniaxial compression test was carried out on the basalt fiber-doped tailing sand cemented filler specimens to analyze the energy dissipation characteristics, and the damage constitutive equations with different basalt fiber contents were established based on damage mechanics. The results show that with the increase of fiber doping and fiber length, the uniaxial compressive strength and ductility of the filling body show a trend of increasing and then decreasing; the optimal value of fiber doping is 0.6%, and the optimal value of fiber length is 9 mm; the total strain energy, elastic strain energy and dissipation energy of basalt fiber-modified tailing sand cemented filling body at peak stress show a trend of increasing and then decreasing, and the energy dissipation energy of the filling body shows a trend of increasing and then decreasing. The energy dissipation energy shows a trend of increasing and then decreasing, and the energy dissipation energy shows a trend of increasing and then decreasing. The total strain energy, elastic strain energy, and dissipation energy at the peak stress show a trend of decreasing after increasing with the fiber doping and fiber length, and the energy damage evolution process can be divided into four stages: no damage stage, stable damage development stage, accelerated damage growth stage, and damage destruction; in addition, the existing damage constitutive model of the fiber-filled body was optimized, and the damage correction factor was introduced to obtain the damage constitutive model of the filled body with different fiber contents, and finally, after the verification of experimental and theoretical models, it was found that the two stress–strain curves coincided well. Finally, after the test and theoretical model verification, it is found that the stress–strain curves of the two are in good agreement, which indicates that the established theoretical model has a certain reference value for engineering practice, and at the same time, it has certain limitations.
Research on Quantification of Structural Natural Frequency Uncertainty and Finite Element Model Updating Based on Gaussian Processes
Authors: Tian Qin , Yao Kai , Cao Shixin
Published: June 2024

During bridge service, material degradation and aging occur, affecting bridge functionality. Bridge health monitoring, crucial for detecting structural damage, includes finite element model modification as a key aspect. Current finite element-based model updating techniques are computationally intensive and lack practicality. Additionally, changes in loading and material property deterioration lead to parameter uncertainty in engineering structures. To enhance computational efficiency and accommodate parameter uncertainty, this study proposes a Gaussian process model-based approach for predicting structural natural frequencies and correcting finite element models. Taking a simply supported beam structure as an example, the elastic modulus and mass density of the structure are sampled by the Sobol sequence. Then, we map the collected samples to the corresponding physical space, substitute them into the finite element model, and calculate the first three natural frequencies of the model. A Gaussian surrogate model was established for the natural frequency of the structure. By analyzing the first three natural frequencies of the simply supported beam, the elastic modulus and mass density of the structure are corrected. The error between the corrected values of elastic modulus and mass density and the calculated values of the finite element model is very small. This study demonstrates that Gaussian process models can improve calculation efficiency, fulfilling the dual objectives of predicting structural natural frequencies and adjusting model parameters.
Transcript-Level Biomarkers of Early Lung Carcinogenesis in Bronchial Lesions
Authors: Pyatnitskiy Mikhail A. , Poverennaya Ekaterina V.
Published: June 2024

Premalignant lesions within the bronchial epithelium signify the initial phases of squamous cell lung carcinoma, posing challenges for detection via conventional methods. Instead of focusing solely on gene expression, in this study, we explore transcriptomic alterations linked to lesion progression, with an emphasis on protein-coding transcripts. We reanalyzed a publicly available RNA-Seq dataset on airway epithelial cells from 82 smokers with and without premalignant lesions. Transcript and gene abundance were quantified using kallisto, while differential expression and transcript usage analysis was performed utilizing sleuth and RATs packages. Functional characterization involved overrepresentation analysis via clusterProfiler, weighted coexpression network analysis (WGCNA), and network analysis via Enrichr-KG. We detected 5906 differentially expressed transcripts and 4626 genes, exhibiting significant enrichment within pathways associated with oxidative phosphorylation and mitochondrial function. Remarkably, transcript-level WGCNA revealed a single module correlated with dysplasia status, notably enriched in cilium-related biological processes. Notable hub transcripts included RABL2B (ENST00000395590), DNAH1 (ENST00000420323), EFHC1 (ENST00000635996), and VWA3A (ENST00000563389) along with transcription factors such as FOXJ1 and ZNF474 as potential regulators. Our findings underscore the value of transcript-level analysis in uncovering novel insights into premalignant bronchial lesion biology, including identification of potential biomarkers associated with early lung carcinogenesis.
Identification of Alcohol Use Prior to Major Cancer Surgery: Timeline Follow Back Interview Compared to Four Other Markers
Authors: Nicklasson Johanna , Sjödell Moa , Tønnesen Hanne , Lauridsen Susanne Vahr , Rasmussen Mette
Published: June 2024

Background: The postoperative complication rate is 30–64% among patients undergoing muscle-invasive and recurrent high-risk non-muscle-invasive bladder cancer surgery. Preoperative risky alcohol use increases the risk. The aim was to evaluate the accuracy of markers for identifying preoperative risky alcohol. Methods: Diagnostic test sub-study of a randomized controlled trial (STOP-OP trial), based on a cohort of 94 patients scheduled for major bladder cancer surgery. Identification of risky alcohol use using Timeline Follow Back interviews (TLFB) were compared to the AUDIT–C questionnaire and three biomarkers: carbohydrate-deficient transferrin in plasma (P–CDT), phosphatidyl-ethanol in blood (B–PEth), and ethyl glucuronide in urine (U–EtG). Results: The correlation between TLFB and AUDIT–C was strong (ρ = 0.75), while it was moderate between TLFB and the biomarkers (ρ = 0.55–0.65). Overall, sensitivity ranged from 56 to 82% and specificity from 38 to 100%. B–PEth showed the lowest sensitivity at 56%, but the highest specificity of 100%. All tests had high positive predictive values (79–100%), but low negative predictive values (42–55%). Conclusions: Despite high positive predictive values, negative predictive values were weak compared to TLFB. For now, TLFB interviews seem preferable for preoperative identification of risky alcohol use.
A 22-G or a 25-G Needle: Which One to Use in the Diagnostics of Solid Pancreatic Lesions? A Systematic Review and Meta-Analysis
Authors: Nawacki Łukasz , Gorczyca-Głowacka Iwona , Zieliński Paweł , Znamirowski Przemysław , Kozłowska-Geller Monika , Ciba-Stemplewska Agnieszka , Kołomańska Magdalena
Published: June 2024

With the 12th highest incidence and a common late diagnostic at advanced stages, neoadjuvant therapies for pancreatic cancer are important, but they require a confirmed diagnosis. Being a diagnostic standard, the clarification of the clinical relevance of needle gauges is needed, as larger ones may retrieve more tissue for diagnostics, but may also increase the risk of complications. We performed a meta-analysis to compare the efficiency of the most commonly used 22-G and 25-G needles for EUS guided biopsy in solid pancreatic lesions. The MEDLINE (via PubMed), Embase, Cochrane (CENTRAL), and Scopus databases were searched with “EUS”, “needle”, “FNA”, “pancreas”, “prospective”, “22G”, and “25G” keywords. Mixed effects were assessed in the model, with a mean of 86% and a 95% confidence interval. Fourteen prospective studies that compared the efficiency of 22-G and 25-G biopsy needles in 508 and 524 lesions, respectively, were analyzed, along with 332 specimens biopsied using both needle sizes. The groups did not significantly differ in the outcomes. A low degree of heterogeneity was observed overall, except for specimen adequacy. Moreover, 22-G and 25-G needles have comparable safety and efficacy for focal pancreatic lesion biopsies without a high risk of complications.
Clinical Outcomes of Online Adaptive Magnetic Resonance-Guided Stereotactic Body Radiotherapy of Adrenal Metastases from a Single Institution
Authors: Hoegen-Saßmannshausen Philipp , Jessen Inga , Buchele Carolin , Schlüter Fabian , Rippke Carolin , Renkamp Claudia Katharina , Weykamp Fabian , Regnery Sebastian , Liermann Jakob , Meixner Eva , Hoeltgen Line , Eichkorn Tanja , König Laila , Debus Jürgen , Klüter Sebastian , Hörner-Rieber Juliane
Published: June 2024

(1) Background: Recent publications foster stereotactic body radiotherapy (SBRT) in patients with adrenal oligometastases or oligoprogression. However, local control (LC) after non-adaptive SBRT shows the potential for improvement. Online adaptive MR-guided SBRT (MRgSBRT) improves tumor coverage and organ-at-risk (OAR) sparing. Long-term results of adaptive MRgSBRT are still sparse. (2) Methods: Adaptive MRgSBRT was performed on a 0.35 T MR-Linac. LC, overall survival (OS), progression-free survival (PFS), overall response rate (ORR), and toxicity were assessed. (3) Results: 35 patients with 40 adrenal metastases were analyzed. The median gross tumor volume was 30.6 cc. The most common regimen was 10 fractions at 5 Gy. The median biologically effective dose (BED10) was 75.0 Gy. Plan adaptation was performed in 98% of all fractions. The median follow-up was 7.9 months. One local failure occurred after 16.6 months, resulting in estimated LC rates of 100% at one year and 90% at two years. ORR was 67.5%. The median OS was 22.4 months, and the median PFS was 5.1 months. No toxicity > CTCAE grade 2 occurred. (4) Conclusions: LC and ORR after adrenal adaptive MRgSBRT were excellent, even in a cohort with comparably large metastases. A BED10 of 75 Gy seems sufficient for improved LC in comparison to non-adaptive SBRT.
Does a “Western Lifestyle” Confer a Higher Burden of Colorectal Cancer? A Comparison of EU15+ Countries versus Global Trends between 1990 and 2019
Authors: Walker Bradley , Jani Chinmay T. , Liu Weitao , Punjwani Shoheera , Kareff Samuel , Ceglowski Peter , Singh Harpreet , Mariano Melissa , Salciccioli Justin D. , Borges Lawrence , Lopes Gilberto
Published: June 2024

The incidence of colorectal cancer (CRC) in the U.S. is declining in adults 50 years and older; however, recent studies suggest an increasing disease burden among adults under age 50. This study aims to compare the incidence, mortality, and mortality-to-incidence ratios (MIRs) of CRC in EU15+ countries to determine if similar age-stratified occurrences are observed across these countries with similar “Western lifestyle”-related risk factors. Incidence and mortality rates for CRC between 1990 and 2019 were extracted using the Global Burden of Disease database. The data were age-stratified into groups between ages 25–49, 50–69, and greater than 69 years. We observed that the incidence of CRC increased globally for all age groups, with the highest increase observed for males (75.9%) and females (27.7%) aged 25–49. A similar trend was observed in 15 of the 19 EU15+ countries for males and 16 of the 19 EU15+ countries for females aged 25–49. Global mortality rates decreased for all age groups in females but increased for males in all age groups. This raises concerns regarding potentially modifiable risk factors contributing to increased CRC development and underscores the importance of implementing standardized screening at an earlier stage to ensure adequate detection in the younger population.
The Histogenetic Origin of Malignant Cells Predicts Their Susceptibility towards Synthetic Lethality Utilizing the TK.007 System
Authors: Pallasch Fabian Bernhard , Freytag Vera , Kriegs Malte , Gatzemeier Dennis , Mair Thomas , Voss Hannah , Riecken Kristoffer , Dawood Mona , Fehse Boris , Efferth Thomas , Schlüter Hartmut , Schumacher Udo
Published: June 2024

Background: Remarkable differences exist in the outcome of systemic cancer therapies. Lymphomas and leukemias generally respond well to systemic chemotherapies, while solid cancers often fail. We engineered different human cancer cells lines to uniformly express a modified herpes simplex virus thymidine kinase TK.007 as a suicide gene when ganciclovir (GCV) is applied, thus in theory achieving a similar response in all cell lines. Methods: Fifteen different cell lines were engineered to express the TK.007 gene. XTT-cell proliferation assays were performed and the IC50-values were calculated. Functional kinome profiling, mRNA sequencing, and bottom-up proteomics analysis with Ingenuity pathway analysis were performed. Results: GCV potency varied among cell lines, with lymphoma and leukemia cells showing higher susceptibility than solid cancer cells. Functional kinome profiling implies a contribution of the SRC family kinases and decreased overall kinase activity. mRNA sequencing highlighted alterations in the MAPK pathways and bottom-up proteomics showed differences in apoptotic and epithelial junction signaling proteins. Conclusions: The histogenetic origin of cells influenced the susceptibility of human malignant cells towards cytotoxic agents with leukemias and lymphomas being more sensitive than solid cancer cells.
Musculoskeletal Disorders Related to Upper Limb Disability after One-Year Lung Cancer Resection
Authors: Martín Núñez Javier , Raya Benítez Julia , Quero Valenzuela Florencio , Calvache Mateo Andrés , Navas Otero Alba , Heredia Ciuró Alejandro , Valenza Marie Carmen
Published: June 2024

Lung resection represents the main curative treatment in lung cancer; however, this surgical process leads to several disorders in tissues and organs. Previous studies have reported cardiovascular, pulmonary, and muscular disturbances that affect the functional capacity of these patients in the short, mid, and long term. However, upper limb impairment has been scarcely explored in the long term, despite the relevance in the independence of the patients. The aim of this study was to characterize the upper limb impairment in survivors of lung cancer one year after pulmonary resection. In this observational trial, patients who underwent lung cancer surgery were compared to control, healthy subjects matched by age and gender. Upper limb musculoskeletal disorders (shoulder range of motion, pain pressure threshold, nerve-related symptoms) and functional capacity (upper limb exercise capacity) were evaluated one-year post-surgery. A total of 76 survivors of lung cancer and 74 healthy subjects were included in the study. Significant differences between groups were found for active shoulder mobility (p < 0.05), widespread hypersensitivity to mechanical pain (p < 0.001), mechanosensitivity of the neural tissue (p < 0.001), and upper limb exercise capacity (p < 0.001). Patients who undergo lung cancer surgery show upper limb musculoskeletal disorders and upper limb functional impairment after a one-year lung resection. This clinical condition could limit the functionality and quality of life of patients with lung cancer.
Exploring the Use of Natural Ingredients for the Protection of Textured Hair from Ultraviolet Radiation: An In Vitro Study
Authors: Markiewicz Ewa , Idowu Olusola C.
Published: June 2024

Human hair is characterized by significant diversity, which is dictated by the individual genetic makeup. It is estimated that up to 70% of the world’s population has textured hair, with a significant proportion also living in areas with high exposure to ultraviolet radiation (UVR). This highlights the unique requirements for textured hair care products that can protect hair shafts from the harmful effects of the environment whilst enhancing the natural features of the fibre. In this study, we investigated the UV-protective effects of hair conditioner formulated from active ingredients, mangiferin, ferulic acid and naringin. The analysis of UV-irradiated straight and textured hair was performed by measurements of hair thickness and darkness, the uptake of the fluorescent dye, spectral absorbance of keratin extracts, ATR-FTIR and SEM. We found that textured hair was more sensitive to UVR-induced changes than straight hair, with the primary molecular targets represented by chemical groups in keratins. Pre-treatment of the hair with conditioner had protective effects against structural damage and oxidative stress caused by UVR. These data indicate the importance of the personalized approach to hair research and the formulation of hair care products tailored to the individual demands of textured hair types.
Soil–Plant–Pollinator Relationships in Urban Grass and Meadow Habitats: Competing Benefits and Demands of Tall Flowering Plants on Soil and Pollinator Diversity
Authors: Rust Will , Sotkewicz Madison , Li Zhaoxing , Mercer Theresa , Johnston Alice S.
Published: June 2024

Urban green spaces can be important habitats for soil, plant, and pollinator diversity and the complementary ecosystem functions they confer. Most studies tend to investigate the relationships between plant diversity with either soil or pollinator diversity, but establishing their relationship across habitat types could be important for optimising ecosystem service provision via alternative management (for instance, urban meadows in place of short amenity grass). Here, we investigate soil–plant–pollinator relationships across urban grass and meadow habitats through a range of measured biodiversity (soil mesofauna and macrofauna, plants, aboveground invertebrates, and pollinators) and edaphic variables. We found significant effects of habitat type on available nutrients (plant and soil C:N ratios) but less clear relationships were observed between habitat type and diversity metrics. Soil–plant–pollinator interactions across habitat types and sites showed an interconnection, whereby flowering plant abundance increased alongside soil macrofauna abundance. Site characteristics that showed strong effects on plant and invertebrate diversity metrics were C:N ratios (plant and soil) and soil pH, suggesting a potential role of nutrient availability on soil–plant–pollinator associations. Our results suggest that a combination of short-mown grass, tall grass, and sown flowers can provide greater benefits for soil and pollination services as each habitat type benefits different taxa due to differing sensitivities to management practices. For example, pollinators benefit from sown flowers but soil fauna are sensitive to annual sowing. Our results also indicate that sown flowers may not optimise overall biodiversity as expected due to disturbance and the depleting role of tall, flowering plants on soil nutrient availability. Future research across a greater range of sites in urban landscapes would resolve the potential role of nutrient availability in modulating soil–plant–pollinator interactions in urban green spaces.
Active Disturbance Rejection Control of Permanent Magnet Synchronous Motor Based on RPLESO
Authors: Zhou Chengpeng , Wang Bo , Liu Kai , Ren Kaixuan
Published: June 2024

In view of the problem of the low-speed jitter of household lawn mowers driven by a permanent magnet synchronous motor (PMSM) at low speeds and high torque, and the complicated parameters of traditional non-linear active disturbance rejection controllers, a partially optimized linear active disturbance rejection control (LADRC) driving PMSM strategy is proposed. First, the linear extended state observer (LESO), which bears a significant burden in terms of speed and load estimation in active disturbance rejection control, is optimized by reducing its order to improve the anti-disturbance performance of the active disturbance rejection controller within a limited bandwidth. Secondly, the reduced-order parallel linear extended state observer (RPLESO) is obtained by optimizing the parallel structure of the order-reduced LESO, which improves the control precision and robustness of the system. Through a simulation and experimental verification, the optimized LADRC control of the PMSM system is shown to improve the parameter adjustability, speed estimation precision and system robustness.
Copper Distribution and Binding Affinity to Size-Fractioned Dissolved and Particulate Organic Matter in River Sediment
Authors: Hung Ming-Yuan , Huang Wei-Hsiang , Tsai Hsiang-Chun , Hsieh Chi-Ying , Chen Ting-Chien
Published: June 2024

This study investigated the distribution of copper in sediment dissolved and particulate organic matter (DOM and POM) based on their size. The DOM and alkaline extracted POM (AEOM) were separated into five size fractions using a cross-flow ultrafiltration (CFUF) system. The results showed that Cu mass was mainly distributed in the low molecular weight (<1 kDa, LMW) fraction of the DOM with an average range of 78.1–83.1%. Conversely, the high molecular weight (1 kDa–0.45 μm, HMW) AEOM fraction had a higher distribution of Cu mass with an average range of 92.6–93.3%. The Cu and AEOM binding affinity ratios (CuBAAEOM) ranged from 17.0 to 149.6 μmol/g-C in site-1 and from 20.6 to 143.7 μmol/g-C in site-2. The HMW CuBAAEOM ratios were significantly higher than the LMW ratios. The Cu and DOM binding affinity ratios (CuBADOM) ranged from 5.6 to 358.6 μmol/g-C and 17.2 to 126.6 μmol/g-C in site-1 and site-2, respectively. However, the LMW CuBADOM ratios were significantly higher than the HMW ratios. Optical indices suggested that the AEOM had more aromaticity and terrestrial and allochthonous contributions than the DOM. The optical indices were significantly correlated with the CuBAAEOM ratios but weakly correlated with the CuBADOM ratios. Sediment exchange between POM and DOM may affect copper distribution. DOM has a low-molecular-weight composition, while POM retains high-molecular-weight organic matter.
Siberian Pine and Larch Response to Warming-Drying Climate in the Southern Boundary of Their Range
Authors: Petrov Ilya A. , Kharuk Viacheslav I. , Golyukov Alexey S. , Im Sergei T. , Ondar Sergei O. , Shushpanov Alexander S.
Published: June 2024

Trees’ growth and areal responses to changing climate are primarily expected within the edges of the species range. Here, we compared the responses of Siberian pine (Pinus sibirica Du Tour), a moisture-sensitive species, and drought-resistant larch (Larix sibirica Ledeb.) at the southern part of their ranges in the Siberian Mountains (the Tannu-Ola Ridge). We study the species’ growth and proportion in the forests from forest-steppe to treeline ecotone along the elevation gradient. These studies are based on radial growth index (GI) analysis and GI dependence on the climate variables. We used satellite time series to detect the land cover changes (areas of larch and Siberian pine, as well as shrubs and birch). We compared trees’ GI before and after warming “restart” in the late 1990s. Generally, GI dependence on the air temperature was negative at elevations below c. 1600 m a.s.l., whereas GI dependence on the moisture variables (precipitation, vapor pressure deficit, and soil moisture) was positive for both species. Above 1600 m, increasing air temperatures stimulated species growth, whereas the influence of moisture variables was negative (for larch) or neutral (for Siberian pine). After the warming restart, the GI of both conifers increased in moisture-sufficient high elevations and treeline ecotone, whereas within low elevations (<1300 m), the GI was stagnant or suppressed. Both species’, especially Siberian pine, negative growth dependence on air temperature and positive dependence on the moisture variables strongly increased since the warming restart. We found a risen growth dependence of both species on the soil-stored water during the previous year (September–October), which smoothed moisture stress at the beginning of the growing season. Yet both species’ growth also suffered as a result of early spring warms. We found that larch is migrating in both uphill and downhill directions, while Siberian pine is migrating uphill only. Forests loss occurred at low elevations (<1300 m), whereas forest and shrub gain occurred at high (>2000 m) ones. The upper boundary of the forests and shrubs is migrating uphill at rates of about 0.8 and 0.3 m/y, respectively. We observed a decrease in Siberian pine proportion in the forests, whereas areas of larch and birch strongly increased (by 150% and 100%, respectively), which indicates the retreat of Siberian pine from its southern habitat. We suggested afforestation of the areas of Siberian pine mortality by the drought-tolerant larch species.
Forest Aboveground Biomass Estimation and Inventory: Evaluating Remote Sensing-Based Approaches
Authors: Khan Muhammad Nouman , Tan Yumin , Gul Ahmad Ali , Abbas Sawaid , Wang Jiale
Published: June 2024

Remote sensing datasets offer robust approaches for gaining reliable insights into forest ecosystems. Despite numerous studies reviewing forest aboveground biomass estimation using remote sensing approaches, a comprehensive synthesis of synergetic integration methods to map and estimate forest AGB is still needed. This article reviews the integrated remote sensing approaches and discusses significant advances in estimating the AGB from space- and airborne sensors. This review covers the research articles published during 2015–2023 to ascertain recent developments. A total of 98 peer-reviewed journal articles were selected under the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) guidelines. Among the scrutinized studies, 54 were relevant to spaceborne, 22 to airborne, and 22 to space- and airborne datasets. Among the empirical models used, random forest regression model accounted for the most articles (32). The highest number of articles utilizing integrated dataset approaches originated from China (24), followed by the USA (15). Among the space- and airborne datasets, Sentinel-1 and 2, Landsat, GEDI, and Airborne LiDAR datasets were widely employed with parameters that encompassed tree height, canopy cover, and vegetation indices. The results of co-citation analysis were also determined to be relevant to the objectives of this review. This review focuses on dataset integration with empirical models and provides insights into the accuracy and reliability of studies on AGB estimation modeling.
Characteristics and Driving Mechanisms of Understory Vegetation Diversity Patterns in Central and Southern China
Authors: Xiao Yaqin , Tian Yuxin , Song Qingan , Deng Nan
Published: June 2024

Large-scale forest restoration projects significantly reduce the net rates of forest loss. However, as a key component of forest restoration, planted forests have failed to restore biodiversity. China has implemented a large-scale afforestation program, which includes pure planted forests in particular, leading to various changes in ecosystem processes. Despite this, a comprehensive analysis of understory vegetation diversity patterns in these pure planted forests is still lacking. This study aimed to analyze the data on understory vegetation diversity from three typical pure and natural forest ecosystems of Hunan ecological forests to reveal their diversity patterns. The results revealed no significant difference in the understory diversity index between natural and pure forest types, although natural forests had a bigger species pool. The Zipf–Mandelbrot model was a better fit for species abundance distribution. The fitted results suggested that both environmental filtering and neutral processes affected the species abundance distribution and pure understory communities during restoration succession. Natural forests had the most stable understory diversity structure, whereas pure Phyllostachys heterocycla (Carr.) Mitford forests had the least stable structure. Multivariate regression tree analysis identified indicator species for each community. The gradient boosting model indicated that isothermality and slope direction were the most important factors affecting diversity. The β-diversity analysis showed that community establishment in the four forest types was affected via different mechanisms. The findings of this study have significant implications for understanding the impact of afforestation on the mechanisms for maintaining diversity.
The Effects of Different Vegetation Restoration Models on Soil Quality in Karst Areas of Southwest China
Authors: Ou Han-Biao , Liu Xiong-Sheng , Wei Shuo-Xing , Jiang Yi , Gao Feng , Wang Zhi-Hui , Fu Wei , Du Hu
Published: June 2024

Rocky desertification is a devastating process in Karst areas of Southwest China and induces serious fragmentation in ecosystems. Therefore, vegetation restoration and the scientific evaluation of soil quality are key restorative strategies in these areas. In this study, a natural closed forest and a disturbed forest with three restoration models, including an evergreen broad-leaved forest, mixed forest, and deciduous forest, were investigated in Huanjiang County. More than nineteen soil properties (including physical, chemical, and biotic properties) were analyzed across treatments, and principal component analyses (PCA) were combined with a minimum data set (MDS) applied to evaluate the soil quality. Our study sought to identify a vegetation restoration model to improve the soil quality in this area. We demonstrated that soil physical and chemical properties, microbial biomass, and enzyme activities significantly differed across all of the models. Soil water content, capillary porosity, total porosity, organic carbon, total phosphorus, available phosphorus, and urease activity were high in the mixed forest, leading to better physical soil properties. Also, relatively high soil total nitrogen, total potassium, available nitrogen, available potassium, microbial biomass C and N, catalase, sucrose, and alkaline phosphatase levels were observed in the deciduous broad-leaved forest, resulting in improved soil chemical properties. Based on the minimum data set (MDS) method, six indicators, including non-capillary porosity, organic carbon, total phosphorus, pH, microbial biomass nitrogen, and urease activity, were selected to evaluate the soil quality across the models. Our data showed that, among the five models, the deciduous broad-leaved forest had the highest soil quality index (0.618), followed by the mixed forest (0.593). Stepwise regression analysis showed that soil organic carbon explained 79.9% of the variations in the soil quality indices, suggesting it was a major factor affecting the soil quality. Thus, vegetation restoration models mainly comprised of native tree species effectively improved the soil quality in Karst rocky desertification areas, with deciduous broad-leaved forests displaying the best effects, followed by mixed forests.