bluuebunny/crossref_metadata_2025 huggingface dataset:
The 2030 Agenda: A Survival Kit for Humanity
Authors: Friberg Peter , Causevic Sara , Dahlstrand Johan , Segersten Ulrica , Tomson Göran
Published: December 2019

To deliver on the 2030 Agenda and the seventeen development goals, while facing complex health challenges, we need research and education that extend across multiple scientific fields. This will enable researchers from a variety of disciplines to meet, identify research issues, apply for funding, and conduct interdisciplinary research. In addition, student involvement is key in achieving the 2030 Agenda’s global goals – and beyond. Challenges include, climate change and child health, non-peaceful societies, gender inequalities and health.

The Swedish Institute for Global Health Transformation (SIGHT) was founded in 2017 at the Royal Swedish Academy of Sciences with the support of the Bill & Melinda Gates Foundation. SIGHT’s mission is to promote an interdisciplinary approach in research and education in the field of global health. In order to deliver on the commitment to global health among researchers and students in various scientific fields and at universities and colleges across Sweden, SIGHT has established SIGHT Fellows, a mentoring programme for academic researchers. In collaboration with universities, established research institutions, and other stakeholders, SIGHT Student Network holds dynamic meetings for students from a variety of disciplines and universities to contribute to delivering the UN’s sustainability goals.
SPECT imaging of lung ischemia-reperfusion injury using [99mTc]cFLFLF for molecular targeting of formyl peptide receptor 1
Authors: Charles Eric J. , Chordia Mahendra D. , Zhao Yunge , Zhang Yi , Mehaffey J. Hunter , Glover David K. , Dimastromatteo Julien , Chancellor W. Zachary , Sharma Ashish K. , Kron Irving L. , Pan Dongfeng , Laubach Victor E.
Published: December 2019

Primary graft dysfunction after lung transplantation, a consequence of ischemia-reperfusion injury (IRI), is a major cause of morbidity and mortality. IRI involves acute inflammation and innate immune cell activation, leading to rapid infiltration of neutrophils. Formyl peptide receptor 1 (FPR1) expressed by phagocytic leukocytes plays an important role in neutrophil function. The cell surface expression of FPR1 is rapidly and robustly upregulated on neutrophils in response to inflammatory stimuli. Thus, we hypothesized that use of [99mTc]cFLFLF, a selective FPR1 peptide ligand, would permit in vivo neutrophil labeling and noninvasive imaging of IRI using single-photon emission computed tomography (SPECT). A murine model of left lung IRI was utilized. Lung function, neutrophil infiltration, and SPECT imaging were assessed after 1 h of ischemia and 2, 12, or 24 h of reperfusion. [99mTc]cFLFLF was injected 2 h before SPECT. Signal intensity by SPECT and total probe uptake by gamma counts were 3.9- and 2.3-fold higher, respectively, in left lungs after ischemia and 2 h of reperfusion versus sham. These values significantly decreased with longer reperfusion times, correlating with resolution of IRI as shown by improved lung function and decreased neutrophil infiltration. SPECT results were confirmed using Cy7-cFLFLF-based fluorescence imaging of lungs. Immunofluorescence microscopy confirmed cFLFLF binding primarily to activated neutrophils. These results demonstrate that [99mTc]cFLFLF SPECT enables noninvasive detection of lung IRI and permits monitoring of resolution of injury over time. Clinical application of [99mTc]cFLFLF SPECT may permit diagnosis of lung IRI for timely intervention to improve outcomes after transplantation.
Inhibition of ATP hydrolysis restores airway surface liquid production in cystic fibrosis airway epithelia
Authors: van Heusden Catharina , Button Brian , Anderson Wayne H. , Ceppe Agathe , Morton Lisa C. , O’Neal Wanda K. , Dang Hong , Alexis Neil E. , Donaldson Scott , Stephan Holger , Boucher Richard C. , Lazarowski Eduardo R.
Published: December 2019

Airway surface dehydration is a pathological feature of cystic fibrosis (CF) lung disease. CF is caused by mutations in the CF transmembrane conductance regulator (CFTR), a cyclic AMP-regulated Cl− channel controlled in part by the adenosine A2B receptor. An alternative CFTR-independent mechanism of fluid secretion is regulated by ATP via the P2Y2 receptor (P2Y2R) that activates Ca2+-regulated Cl− channels (CaCC/TMEM16) and inhibits Na+ absorption. However, due to rapid ATP hydrolysis, steady-state ATP levels in CF airway surface liquid (ASL) are inadequate to maintain P2Y2R-mediated fluid secretion. Therefore, inhibiting airway epithelial ecto-ATPases to increase ASL ATP levels constitutes a strategy to restore airway surface hydration in CF. Using [γ32P]ATP as radiotracer, we assessed the effect of a series of ATPase inhibitory compounds on the stability of physiologically occurring ATP concentrations. We identified the polyoxometalate [Co4(H2O)2(PW9O34)2]10− (POM-5) as the most potent and effective ecto-ATPase inhibitor in CF airway epithelial cells. POM-5 caused long-lasting inhibition of ATP hydrolysis in airway epithelia, which was reversible upon removal of the inhibitor. Importantly, POM-5 markedly enhanced steady-state levels of released ATP, promoting increased ASL volume in CF cell surfaces. These results provide proof of concept for ecto-ATPase inhibitors as therapeutic agents to restore hydration of CF airway surfaces. As a test of this notion, cell-free sputum supernatants from CF subjects were studied and found to have abnormally elevated ATPase activity, which was markedly inhibited by POM-5.
Adipose-Derived Stromal Cells Attenuate Adipose Inflammation in Obesity through Adipocyte Browning and Polarization of M2 Macrophages
Authors: Zhang Wen-Chao , Qin Feng , Wang Xiao-Jun , Liu Zhi-Fei , Zhu Lin , Zeng Ang , Zhang Ming-Zi , Yu Nan-Ze , Long Xiao
Published: December 2019

Obesity is a metabolic condition associated with multiple health problems such as endocrine and metabolic dysfunction and chronic inflammation in adipose tissues. In this study, the ADSCs could be stimulated to differentiate into brown adipocyte with rosiglitazone treatment based on the Oil-Red-O staining trial. Furthermore, the multilocular lipid droplets located in the center was increased in differentiated brown adipocytes, and brown fat-associated proteins, UCP1, PPAR-γ, and LPL were highly expressed in brown adipocytes differentiated from ADSCs. Additionally, the results of animal experiments showed that both weight and amount of VLDL and LDL were decreased in the serum of obese mice after transplantation of rosiglitazone-induced brown adipocytes, while the level of HDL increased. Moreover, the proteins associated with lipid metabolism, LPA and UCP1, were downregulated, and the inflammatory response was suppressed through inhibition of the ITGAM/NF-κB-mediated proinflammatory responses and polarization of M2 macrophages. Similarly, the amounts of proinflammatory cytokines, TNF-α, IL-6, and IL-1β were decreased after rosiglitazone-induced brown adipocyte transplantation. On the contrary, anti-inflammatory cytokine IL-10 was significantly increased in both groups of obese mice, with or without brown adipocyte transplantation. Therefore, the adipose-derived stromal cells with induced browning could promote lipid consumption and alternative polarization of M2 macrophages to attenuate adipose inflammation in obesity mouse models, which thus provides a potential therapy for obesity.
Medicinal Plants in the Prevention and Treatment of Colon Cancer
Authors: Aiello Paola , Sharghi Maedeh , Mansourkhani Shabnam Malekpour , Ardekan Azam Pourabbasi , Jouybari Leila , Daraei Nahid , Peiro Khadijeh , Mohamadian Sima , Rezaei Mahdiyeh , Heidari Mahdi , Peluso Ilaria , Ghorat Fereshteh , Bishayee Anupam , Kooti Wesam
Published: December 2019

The standard treatment for cancer is generally based on using cytotoxic drugs, radiotherapy, chemotherapy, and surgery. However, the use of traditional treatments has received attention in recent years. The aim of the present work was to provide an overview of medicinal plants effective on colon cancer with special emphasis on bioactive components and underlying mechanisms of action. Various literature databases, including Web of Science, PubMed, and Scopus, were used and English language articles were considered. Based on literature search, 172 experimental studies and 71 clinical cases on 190 plants were included. The results indicate that grape, soybean, green tea, garlic, olive, and pomegranate are the most effective plants against colon cancer. In these studies, fruits, seeds, leaves, and plant roots were used forin vitroandin vivomodels. Various anticolon cancer mechanisms of these medicinal plants include induction of superoxide dismutase, reduction of DNA oxidation, induction of apoptosis by inducing a cell cycle arrest in S phase, reducing the expression of PI3K, P-Akt protein, and MMP as well; reduction of antiapoptotic Bcl-2 and Bcl-xL proteins, and decrease of proliferating cell nuclear antigen (PCNA), cyclin A, cyclin D1, cyclin B1 and cyclin E. Plant compounds also increase both the expression of the cell cycle inhibitors p53, p21, and p27, and the BAD, Bax, caspase 3, caspase 7, caspase 8, and caspase 9 proteins levels. In fact, purification of herbal compounds and demonstration of their efficacy in appropriatein vivomodels, as well as clinical studies, may lead to alternative and effective ways of controlling and treating colon cancer.
A New Evaluation Method for the Fracability of a Shale Reservoir Based on the Structural Properties
Authors: Sui Haoyue , Gao Wei , Hu Ruilin
Published: December 2019

The fracability of shale reservoirs is one of the key indicators used for evaluating whether or not the shale can be used as a “sweet spot zone.” It has been determined that the structural properties of rock have important influences on the evaluation of the fracability of reservoirs. In the current study, five rock quality designation (RQD) calculation methods were compared and analyzed for the purpose of selecting an RQDI for characterizing rock structures. Focused on the lack of structural factors included in the previous fracability evaluation methods, a new model for fracability evaluation based on the combination of the brittleness index, structural index, and fracture toughness was constructed using a linear elastic fracture theory. The model showed that good fracability not only included higher brittleness but also required less energy to produce cracks. Meanwhile, good fracability also required more discontinuous structural planes. In the current study, a formation with a higher fracability index was considered to be a fractureable interval and a formation with a lower fracability index was a fracture barrier. Finally, the reservoir fracability index was modeled using the Xike 2 well in the north of Guizhou Province as a case study. Subsequently, a fracability logging evaluation method based on the fracability index model was determined, which will potentially provide a new technical tool for future fracturing optimization processes.
Piezoresistive Breathing Sensing System with 3D Printed Wearable Casing
Authors: Vanegas Erik , Igual Raul , Plaza Inmaculada
Published: December 2019

Respiratory rate is an important parameter for many health, home care, work, or sport applications. In this paper, a new wearable sensing system based on a piezoresistive FlexiForce sensor has been developed. The sensor can be attached to any common chest strap. A compact 3D casing has been designed and printed with a 3D printer. This casing integrates the sensor and all auxiliary elements of the system: microcontroller, battery, Bluetooth module, connections, battery charger, and acquisition circuit. To the best of our knowledge, this is the first study presenting a FlexiForce respiration sensor that includes all system elements in a single compact casing. The source files with the design of the casing have been published as supplementary material to be reused by any interested researcher. The sensing system was tested with twenty-one subjects for different breathing rates. Two different algorithms were developed to obtain the respiratory rate from the voltage signals recorded by the sensor. Statistical tests were performed to determine the optimal computation time window and algorithm. This approach is also novel in this field. Low error values were obtained for a time window of 27 s with an algorithm based on the calculation of time between zero-crossings (4.02%) and with an algorithm based on counting them (3.40%). To promote research transparency and reusability, the dataset with the recorded data and the source code of the algorithms and statistical tests have also been published. Therefore, an open, replicable, low-error, wearable, wireless, and compact sensing system to measure respiratory rate was developed and tested.
Effects of Deoxyschisandrin on Visceral Sensitivity of Mice with Inflammatory Bowel Disease
Authors: Xu Zhili , Zhang Mingbo , Dou Deqiang , Kang Tingguo , Li Feng
Published: December 2019

The aims of this study were to build an IBD mouse model and further to observe the effects of deoxyschisandrin on IBD and visceral sensitivity and to evaluate the relevance of brain-derived neurotrophic factor (BDNF) to intestinal hypersensitivity of IBD mice. The results showed that deoxyschisandrin could depress the contraction of isolated smooth muscle, modulate gastrointestinal function, and efficiently decrease the disease activity index (DAI) of IBD mice, which proved that deoxyschisandrin had antidiarrheal effects on the animals. In the colorectal distention (CRD) experiment, visceral sensibility was increased in the model group. However, abdominal withdrawal reflex (AWR) scores were decreased after deoxyschisandrin intervention, indicating that deoxyschisandrin could reduce the visceral hypersensitivity of IBD mice. Both IHC observation and western blotting analysis showed that BDNF protein expression increased evidently in colon of IBD mice. After the intervention of deoxyschisandrin, colon mucosa BDNF protein expression in IBD mice decreased, indicating that deoxyschisandrin could decrease mouse intestinal sensitivity by reducing colon mucosa BDNF expression. In conclusion, deoxyschisandrin possessed antidiarrheal effects and visceral hypersensitivity inhibitory effects in the mice with IBD induced by TNBS, which was related to the reduction in BDNF expression in the colon.
The Analytical Method to Compute the Strain on the Soft PSD in Double-Pulse SRM
Authors: Wang Chunguang , Xu Guiyang
Published: December 2019

In order to obtain the analytical method to compute the circumferential strain on a soft pulse separation device (PSD), deformation processes of the middle section of the soft PSD, the medicine propellant grain and the case are simplified into a two-dimensional plane strain state. It is found that the main factors affecting the circumferential strain of the soft PSD are the circumferential strain of the inner surface of the propellant grain and the gap between the soft PSD and the propellant grain. In order to study the failure mechanism of the soft PSD in the double-pulse solid rocket motor (SRM), a two-dimensional axisymmetric finite element method (FEM) model of the stress process of the soft PSD is established. The variation of the strain of the soft PSD with the internal pressure load is obtained. It is found that the excessive circumferential strain is the main reason for the failure of the soft PSD. Comparing the analytical calculations with the FEM results, it can be found that the analytical method value is slightly higher than the FEM value, so the analytical method results can be used to initially estimate the circumferential strain of the soft PSD and then predict the rationality and feasibility of the design scheme. In order to further study the failure mechanism of the soft PSD, a micro-CT test of in situ stretching of the soft PSD material is carried out and the variation of porosity and elongation of the material is studied. The test results showed that when the material elongation is large, the microinterface debonding rapidly expands into a penetrating damage, and the PSD structure fails. The conclusions obtained in this paper can provide a useful reference for the design of double-pulse SRM.
Effect of Lifestyle Factors on Hypertension by Constitution Type: A Large Community-Based Study
Authors: Park Ji-Eun , Shin Chol , Lee Siwoo
Published: December 2019

Background. The risk of hypertension differs according to lifestyle factors and individual constitution types. The aim of this study was to investigate the effect of lifestyle factors on hypertension and to assess whether those effects differ according to the constitution types. Methods. A total of 5,793 men and women were recruited between 2012 and 2014. Odds ratios for hypertension associated with constitution types and lifestyle factors were estimated. Lifestyle factors included smoking status, body mass index, alcohol consumption, physical activity, and sleep quality. Constitution types were estimated based on the Sasang constitutional medicine as the TE type, SE type, and SY type. Results. The risk of hypertension was significantly higher for SY (odds ratio 1.25 (95% confidence interval 1.03 to 1.52) and TE types (1.38 (1.10 to 1.74)) than the SE type even with adjustment of health behaviors. Compared with individuals who had an unhealthy lifestyle, those with healthy lifestyle scores showed significantly lower risk of hypertension in only SY (odds ratio 0.62 (95% confidence interval 0.48 to 0.81)) and TE types (0.69 (0.58 to 0.81)). The difference in risk for hypertension among constitution types was decreased with a healthy lifestyle (1.34 in SY and 2.35 in TE types, as compared with the SE type) versus an unhealthy lifestyle (2.21 in SY and 3.64 in TE types, as compared with the SE type). Conclusion. The risk of hypertension was different by Sasang constitution types. The impact of lifestyle factors differed according to Sasang constitution types, and the difference in risk of hypertension among constitution types was decreased with a healthy lifestyle.
Green Tea Prevents NAFLD by Modulation of miR-34a and miR-194 Expression in a High-Fat Diet Mouse Model
Authors: Torres L. F. , Cogliati B. , Otton R.
Published: December 2019

Background/Aims. Nonalcoholic fatty liver disease (NAFLD) is considered the hepatic manifestation of metabolic syndrome. It is currently the most common chronic liver disease with complex pathogenesis and challenging treatment. Here, we investigated the hepatoprotective role of green tea (GT) and determined the involvement of miRNAs and its mechanism of action. Methods. Male C57Bl/6 mice were fed with a high-fat diet for 4 weeks. After this period, the animals received gavage with GT (500 mg/kg body weight) over 12 weeks (5 days/week). HepG2 cell lines were transfected with miR-34a or miR-194 mimetics and inhibitors to validate the in vivo results or were treated with TNF-α to evaluate miRNA regulation. Results. GT supplementation protects against NAFLD development by altering lipid metabolism, increasing gene expression involved in triglycerides and fatty acid catabolism, and decreasing uptake and lipid accumulation. This phenotype was accompanied by miR-34a downregulation and an increase in their mRNA targets Sirt1, Pparα, and Insig2. GT upregulated hepatic miR-194 by inhibiting TNF-α action leading to a decrease in miR-194 target genes Hmgcs/Apoa5. Conclusion. Our study identified for the first time that the beneficial effects of GT in the liver can be due to the modulation of miRNAs, opening new perspectives for the treatment of NAFLD focusing on epigenetic regulation of miR-34a and miR-194 as green tea targets.
Nanoreinforcements of Two-Dimensional Nanomaterials for Flame Retardant Polymeric Composites: An Overview
Authors: Lu Shaolin , Hong Wei , Chen Xudong
Published: December 2019

Polymer materials are ubiquitous in daily life. While polymers are often convenient and helpful, their properties often obscure the fire hazards they may pose. Therefore, it is of great significance in terms of safety to study the flame retardant properties of polymers while still maintaining their optimal performance. Current literature shows that although traditional flame retardants can satisfy the requirements of polymer flame retardancy, due to increases in product requirements in industry, including requirements for durability, mechanical properties, and environmental friendliness, it is imperative to develop a new generation of flame retardants. In recent years, the preparation of modified two-dimensional nanomaterials as flame retardants has attracted wide attention in the field. Due to their unique layered structures, two-dimensional nanomaterials can generally improve the mechanical properties of polymers via uniform dispersion, and they can form effective physical barriers in a matrix to improve the thermal stability of polymers. For polymer applications in specialized fields, different two-dimensional nanomaterials have potential conductivity, high thermal conductivity, catalytic activity, and antiultraviolet abilities, which can meet the flame retardant requirements of polymers and allow their use in specific applications. In this review, the current research status of two-dimensional nanomaterials as flame retardants is discussed, as well as a mechanism of how they can be applied for reducing the flammability of polymers.
Reactive Eccrine Syringofibroadenoma on the Heel, Clinically Mimicking Squamous Cell Carcinoma
Authors: Sugita Yuri , Makino Teruhiko , Matsui Kotaro , Shimizu Tadamichi
Published: December 2019

The authors present a case of eccrine syringofibroadenoma that clinically mimicked squamous cell carcinoma and briefly comment on the current knowledge about its clinical and histopathological features and therapeutic options.
The First Case of Native Mitral Valve Endocarditis due to Micrococcus luteus and Review of the Literature
Authors: Khan Alisha , Aung Thu Thu , Chaudhuri Debanik
Published: December 2019

Gram-positive cocci species, notably Staphylococcus, Streptococcus, and Enterococcus account for 80 to 90% of infective endocarditis cases. HACEK microorganisms (Haemophilus spp., Aggregatibacter actinomycetemcomitans, Cardiobacterium hominis, Eikenella corrodens, and Kingella kingae) account for approximately 3% of cases and Candida species account for 1-2% of cases. Micrococcus luteus is a rare cause of endocarditis. To our knowledge, only 17 cases of prosthetic valve endocarditis have been described due to M. luteus and a single case of native aortic valve endocarditis has been described. The following case is the only documented case of native mitral valve endocarditis. A review of the literature pertaining to Micrococcus endocarditis was performed to further characterize the entity.
Performance Analysis of Target Depth Classification Algorithm Based on Sea Experiment Data
Authors: Zhao Anbang , Bi Xuejie , Li Nansong , Zhang Minghui , Piao Shengchun
Published: December 2019

Cross-spectrum signals can be calculated by the pressure signals. The sign distribution of cross-spectrum active component can be effectively used for target depth classification algorithm. The algorithm is applicable for depth classification of targets where frequencies can only excite the first two normal modes. The corresponding research results are mainly based on the theoretical study. There are few researches on the algorithm performance based on experiment results. To overcome this research lack, based on the effective depth model, the effects on various receiving depth, source frequency, and received signal-to-noise ratio on the algorithm performance have been studied in this paper. The influence of sound velocity profile parameters (negative gradient, thermocline intensity, thermocline thickness, and up-boundary depth) on the algorithm performance has also been researched. According to the simulation results, proper adjustment of the receiving depths can effectively improve the algorithm performance. The source frequency primarily affects the position of the ideal receiving depth which can be appropriately adjusted according to the depth classification requirements of the real sea environment. The algorithm performance improves gradually with the increase of signal-to-noise ratio. Moreover, the algorithm can also be applied under the conditions of negative gradient and thermocline. The comprehensive sound velocity profile parameters have a large impact on the depth classification performance of the algorithm. Even in the case of strong negative gradient or strong thermocline, the robustness of the algorithm is still high. The feasibility of our presented method has been verified by sea experiment. The practical application value of the ideal receiving depth has been researched and validated. The factors affecting the algorithm performance including line spectrum continuity and received signal-to-noise ratio have also been analyzed in our simulation and real sea experiments.
Methodology Proposal of EMG Hand Movement Classification Based on Cross Recurrence Plots
Authors: Aceves-Fernandez M. A. , Ramos-Arreguin J. M. , Gorrostieta-Hurtado E. , Pedraza-Ortega J. C.
Published: December 2019

Dealing with electromyography (EMG) signals is often not simple. The nature of these signals is nonstationary, noisy, and high dimensional. These EMG characteristics make their predictability even more challenging. Cross recurrence plots (CRPs) have demonstrated in many works their capability of detecting very subtle patterns in signals often buried in a noisy environment. In this contribution, fifty subjects performed ten different hand movements with each hand with the aid of electrodes placed in each arm. Furthermore, the nonlinear features of each subject’s signals using cross recurrence quantification analysis (CRQA) have been performed. Also, a novel methodology is proposed using CRQA as the mainstream technique to detect and classify each of the movements presented in this study. Additional tools were presented to determine to which extent this proposed methodology is able to avoid false classifications, thus demonstrating that this methodology is feasible to classify surface EMG (SEMG) signals with good accuracy, sensitivity, and specificity. Lastly, the results were compared with traditional machine learning methods, and the advantages of using the proposed methodology above such methods are highlighted.
Reproduction of Local Strong Wind Area Induced in the Downstream of Small-Scale Terrain by Computational Fluid Dynamic (CFD) Approach
Authors: Uchida Takanori , Araki Keiji
Published: December 2019

In this research, the computational fluid dynamic (CFD) approach was applied for the solution of the problems of local strong wind areas in railway fields, and the mechanism of wind generation was discussed. The problem of local wind occurring on a railway line in winter was taken up in this research. A computational simulation for the prediction of wind conditions by large-eddy simulation (LES) was implemented, and it was clarified that local strong wind areas are mainly caused by separated flows originating from small-scale terrain positioned at its upstream (at approximately 180 m above sea level). Meanwhile, the effects of the size of the calculation area and spatial grid resolution on the result of calculation and the effect of atmospheric stability were also discussed. It was clarified that in order to simulate the air flow characteristic of the separated flow originating from the small-scale terrain (at an altitude of approximately 180 m) targeted in the present research, approximately 10 m of spatial resolution of computational cell in the horizontal direction is required. In addition, the effect of stable stratification on the flow was also examined. As a result, lee waves were excited at the downstream of the terrain over time in the case of stably stratified flow (Fr = 1.0). The reverse-flow region lying behind the terrain, which had been observed at a neutral time, was strongly inhibited. Consequently, a local strong wind area was generated at the downstream of the terrain, and a strong wind area passing through the observation mast was observed. By investigating the increasing rate of speed of the local strong wind area induced at the time of stable stratification, it was found that the wind was approximately 1.2 times stronger than what was generated at a neutral time.
Risk Behaviors in Teens with Chronic Kidney Disease: A Study from the Midwest Pediatric Nephrology Consortium
Authors: Xiao Nianzhou , Stolfi Adrienne , Malatesta-Muncher Rossana , Bholah Reshma , Kogon Amy , Eddington Angelica , Chand Deepa , Greenbaum Larry A. , Hanevold Coral , Tran Cheryl L , Chishti Aftab , Davis Keefe , Matloff Robyn , Woroniecki Robert , Klosterman Colleen , Luckritz Kera , Omoloja Abiodun
Published: December 2019

Introduction. There is a paucity of information about risk behaviors in adolescents with chronic kidney disease (CKD). We designed this study to assess the prevalence of risk behaviors among teens with CKD in the United States and to investigate any associations between risk behavior and patient or disease characteristics. Methods. After informed consent, adolescents with CKD completed an anonymous, confidential, electronic web-based questionnaire to measure risk behaviors within five domains: sex, teen driving, alcohol and tobacco consumption, illicit drug use, and depression-related risk behavior. The reference group was composed of age-, gender-, and race-matched US high school students. Results. When compared with controls, teens with CKD showed significantly lower prevalence of risk behaviors, except for similar use of alcohol or illicit substances during sex (22.5% vs. 20.8%, p=0.71), feeling depressed for ≥2 weeks (24.3% vs. 29.1%, p=0.07), and suicide attempt resulting in injury needing medical attention (36.4% vs. 32.5%, p=0.78). Furthermore, the CKD group had low risk perception of cigarettes (28%), alcohol (34%), marijuana (50%), and illicit prescription drug (28%). Use of two or more substances was significantly associated with depression and suicidal attempts (p<0.05) among teens with CKD. Conclusions. Teens with CKD showed significantly lower prevalence of risk behaviors than controls. Certain patient characteristics were associated with increased risk behaviors among the CKD group. These data are somewhat reassuring, but children with CKD still need routine assessment of and counselling about risk behaviors.
Fault Diagnosis of Bearings Based on KJADE and VNWOA‐LSSVM Algorithm
Authors: Wu Tao , Liu Chang Chun , He Cheng
Published: December 2019

In order to accurately diagnose the faulty parts of the rolling bearing under different operating conditions, the KJADE (Kernel Function Joint Approximate Diagonalization of Eigenmatrices) algorithm is proposed to reduce the dimensionality of the high‐dimensional feature data. Then, the VNWOA (Von Neumann Topology Whale Optimization Algorithm) is used to optimize the LSSVM (Least Squares Support Vector Machine) method to diagnose the fault type of the rolling bearing. The VNWOA algorithm is used to optimize the regularization parameters and kernel parameters of LSSVM. The low‐dimensional nonlinear features contained in the multidomain feature set are extracted by KJADE and compared with the results of PCA, LDA, KPCA, and JADE methods. Finally, VNWOA‐LSSVM is used to identify bearing faults and compare them with LSSVM, GA‐LSSVM, PSO‐LSSVM, and WOA‐LSSVM. The results show that the recognition rate of fault diagnosis is up to 98.67% by using VNWOA‐LSSVM. The method based on KJADE and VNWOA‐LSSVM can diagnose and identify fault signals more effectively and has higher classification accuracy.
Further Evidence for the Implication of the MET Gene in Non-Syndromic Autosomal Recessive Deafness
Authors: Bousfiha Amale , Riahi Zied , Elkhattabi Lamiae , Bakhchane Amina , Charoute Hicham , Snoussi Khalid , Bonnet Crystel , Petit Christine , Barakat Abdelhamid
Published: December 2019

Mutations in the mesenchymal epithelial transition factor (MET) gene are frequently associated with multiple human cancers but can also lead to human non-syndromic autosomal recessive deafness (DFNB97). In the present study, we identified a novel homozygous missense mutation in the METgene causing a non-syndromic hearing impairment DFNB97 form. Whole-exome sequencing was performed to determine the genetic causes of hearing loss in a Moroccan consanguineous family with an affected daughter. The structural analysis of native and mutant in the SEMA domain of the MET receptor was investigated using a molecular dynamics simulation (MDS) approach. We identified a novel pathogenic homozygous c.948A>G (p.Ile316Met) mutation in the MET gene in one deaf Moroccan young girl carrying a total bilateral non-syndromic hearing impairment. The results of the MDS approach show that an Ile316Met mutation in the SEMA domain leads to protein flexibility loss. This may produce a major impact on the structural conformation of the MET receptor, which also affects the function and binding site of the receptor. This is the first time that a mutation in the MET gene is described in a Moroccan family. Moreover, this study reports the second family in the world associating deafness and mutation in the MET gene.
The Spectrum of Aggressive Mastocytosis: A Workshop Report and Literature Review
Authors: Leguit Roos , Hebeda Konnie , Kremer Marcus , van der Walt Jon , Gianelli Umberto , Tzankov Alexandar , Orazi Attilio
Published: December 2019

Most cases of mastocytosis are indolent, usually cutaneous mastocytosis or indolent systemic mastocytosis (SM). Aggressive mast cell (MC) diseases are very rare and often fatal. They can develop de novo or due to progression of indolent forms and can present in different ways; either as MC sarcoma or as advanced SM which includes aggressive SM, MC leukemia, and SM with an associated hematological neoplasm. This review will describe these different aggressive forms of mastocytosis, illustrated by cases submitted to the workshop of the 18th Meeting of the European Association for Haematopathology, Basel 2016, organized by the European Bone Marrow Working Group. In addition, the diagnostic criteria for identifying myelomastocytic leukemia, an aggressive myeloid neoplasm with partial MC differentiation that falls short of the criteria for SM, and disease progression in patients with established mastocytosis are discussed.
When Genetics Meets Religion: What Scientists and Religious Leaders Can Learn from Each Other
Authors: Modell Stephen M. , Citrin Toby , Burmeister Margit , Kardia Sharon L.R. , Beil Adelyn , Raisky Jeremy ,   Genetics and Faith Dialogue Group
Published: December 2019

Introduction: To date scientists and religious leaders have not yet engaged in sustained face-to-face conversation concerning precision public health-related genetic technologies. Objectives: To elucidate areas of commonality and divergence in scientists’ and religious leaders’ views of precision genetic technologies, and extract lessons conveyed by religious leaders to scientists, and scientists to religious leaders through participatory dialogue. Methods: Six 1.5-h dialogue sessions were held between 6 religious leaders, 8 University of Michigan scientists, and 3 additional public health/genetic counseling graduate students between October 2016 and September 2017, followed by an open conference at the Ann Arbor Public Library (n = 46). Statements were organized into thematically arranged duets comparing views of scientists and religious leaders. Duets were further ordered into interpretive levels. Comparative techniques were used to assure category agreement and face validity. Results: The analysis yielded 20 duets and 3 interpretive levels (expositional; implications and consequences; and integrative, bridging concepts). Scientists emphasized the value of epigenetic testing for health promotion, and cost saving for some forms of early genetic testing for adult-onset conditions. Religious leaders stressed care for an individual’s willingness to change over technical fixes for behavioral conditions and, together with public participants, the importance of allocating money for societal needs. Both expressed caution on the use of nuclear transfer for mitochondrial DNA replacement and secondary uses of genetic data. Lay conference participants pointed towards a middle ground on the release of genetically edited mosquitoes for disease eradication. Discussion: Scientists stressed the value of professional guidance; religious leaders listened to family needs. Dialogues met four literature-based criteria for stakeholder involvement in deliberative processes. Conclusion: While scientists and religious leaders differ in their points of emphasis and faith orientations (professional competency versus drawing on compassion), they can successfully collaborate in reaching mutual understanding and specific areas of agreement on precision genetic technologies relating to public health.
Additive Value of Transrectal Systematic Ventral Biopsies in Combination with Magnet Resonance Imaging/Ultrasound Fusion-Guided Biopsy in Patients with 3 or More Negative Prostate Biopsies
Authors: Maxeiner Andreas , Nest Alexander M. , Stephan Carsten , Cash Hannes , Baur Alexander D.J. , Fischer Thomas , Kilic Ergin , Piper Sophie K. , Nowak Claus-P. , Busch Jonas , Miller Kurt , Mang Josef
Published: December 2019

Introduction: Patients with consistent suspicion for prostate cancer (PCa) and multiple negative prebiopsies prior to multiparametric magnetic resonance imaging (mpMRI) are still frequently evaluated for an image-guided biopsy and are reported with heterogeneous detection rates. The inclusion of a systematic biopsy (SB) is also still recommended with predominant sampling within the posterior/peripheral zone of the prostate. The aim of this study was (I) to evaluate PCa detection rates using a modified 10 core SB template including anterior biopsies in combination with mpMRI/ultrasound fusion-guided targeted biopsy (TB) in patients with 3 or more negative prebiopsies and (II) to compare mpMRI index lesion localization with histologically confirmed locali­zation from associated prostatectomy samples. Methods: Overall 1,337 consecutive patients underwent sensor-based registration TB of the prostate and a subsequent 10-core SB between January 2012 and December 2015 at our institution. For this study, 101 patients with ≥3 negative prebiopsies and prostate imaging – reporting data system lesions ≥3 were pooled prospectively and underwent TB and a modified SB including 2 ventral (anterior) biopsies. Detection rates were estimated for the modified SB, TB, and its combination. A subgroup analysis of 35 patients undergoing prostatectomy was performed by a head-to-head comparison of mpMRI index lesion and histologically confirmed PCa index lesion localization. Results: The overall detection rate for PCa was 54.5%. The combination of TB and SB detected 14 (25.4%) more cases missed by TB alone (p < 0.001) and 7 (12.7%) more cases missed by SB alone (p = 0.016), respectively. A postoperative Gleason upgrade was seen in 12/35 (34.3%) cases within the TB group and in 14/35 (40.0%) in the SB group, respectively. The subgroup analysis showed a predominant location of PCa index lesions anteriorly at the level of the midgland. The MRI detection rate of the anteriorly located index lesions was 70.4% (15/21 cases) with a clinically significant Gleason score (≥3 + 4 = 7a [International Society of Urological Pathology grade 2]) in 80.9%. Interestingly a modified SB template detected 90.5% (19/21) of the anteriorly located index lesions. Conclusion: Our data suggest that in patients with multiple prebiopsies PCa seems to be predominantly located anteriorly. We suggest the general integration of anterior biopsies despite TB in repeat biopsy patients.
The Efficacy and Safety of Epicutaneous Immunotherapy for Allergic Diseases: A Systematic Review and Meta-Analysis
Authors: Xiong Limei , Lin Jilei , Luo Yin , Chen Wencong , Dai Jihong
Published: December 2019

Objectives: To systematically review the effect and safety of epicutaneous immunotherapy (EPIT) for allergic diseases. Methods: We searched PubMed, EMBASE, Cochrane Central Register of Controlled Trials, China National Knowledge Infrastructure, CQ VIP Database, Wanfang Data, and international trial register from their inception to July 29, 2019, without language restrictions, for randomized controlled trials (RCTs) that compared EPIT versus no EPIT for allergen-triggered allergic reactions. We assessed certainty of evidence by the GRADE approach. Results: Ten RCTs with 1,085 participants (aged from 10 months to 65 years) comparing EPIT with placebo for peanut, cow milk, or grass-pollen allergy met the eligibility criteria. A substantial benefit in terms of desensitization in EPIT group was more likely for peanut or cow milk protein allergy (risk ratio [RR] 2.34, 95% CI 1.69–3.23; I2 = 0%; high certainty evidence). EPIT increased local-treatment-related adverse events (L-TRAE; RR 1.56, 95% CI 1.03–2.36; I2 = 82%; moderate certainty evidence). But there were no significantly increased risk of any TRAEs (low certainty evidence) or systemic-TRAEs (S-TRAEs; very low certainty evidence) in EPIT group. The incidence rate of serious AEs, the use of rescue medications, and anaphylactic reactions stratified by organ systems including skin and mucosa, eyes and upper respiratory, lower respiratory, and gastrointestinal system in EPIT group were similar to placebo group. In subgroup analysis, desensitization of EPIT was significantly effective in peanut allergy (RR 2.29, 95% CI 1.64–3.21; I2 = 0%) and children <12 years (RR 2.85, 95% CI 1.92–4.24; I2 = 0%) with high certainty evidence. Only epicutaneous grass-pollen immunotherapy significantly increased the risk of S-TRAE (RR 4.65, 95% CI 1.10–19.64; I2 = 0%). Conclusion: The systematic review suggests that EPIT might induce desensitization in peanut allergy and an increased risk of local AEs. These findings should be interpreted with caution owing to the limited study and heterogeneity. More data in the older (children ≥12 years and adults) and other allergic diseases are needed.
Contrast Volume to Glomerular Filtration Ratio and Acute Kidney Injury among ST-Segment Elevation Myocardial Infarction Patients Treated with Primary Percutaneous Coronary Intervention
Authors: Zahler David , Rozenfeld Keren-Lee , Merdler Ilan , Peri Yogev , Shacham Yacov
Published: December 2019

Introduction: The ratio of contrast media volume to glomerular filtration rate (contrast/GFR) has been shown to correlate with the occurrence of contrast-induced acute kidney injury (CI-AKI) in unselected patient populations who underwent percutaneous coronary intervention (PCI). Objective: We evaluated the possible utilization of this marker and optimal cutoff among ST-elevation myocardial infarction (STEMI) patients undergoing primary PCI. Methods: We retrospectively included 419 patients with STEMI treated with primary PCI. The occurrence of CI-AKI was defined by the KDIGO criteria as an increase in serum creatinine of ≥0.3 mg/dL within 48 h following PCI. A receiver-operator characteristic (ROC) curve was used to identify the optimal cutoff value of contrast/GFR ratio to predict CI-AKI. This value was then assessed using multivariable logistic regression. Results: The overall incidence of CI-AKI was 9%. The contrast/GFR ratio was significantly higher among patients with CI-AKI (2.7 ± 1.2 vs. 1.9 ± 0.9; p < 0.001). According to the ROC curve analysis, the optimal cutoff value of contrast/GFR ratio to predict AKI was measured as ≥2.13, with 70% sensitivity and 60% specificity (AUC 0.65, 95% CI 0.56–0.74; p = 0.002). In a multivariate logistic regression model, contrast/GFR ratio ≥2.13 was independently associated with CI-AKI (OR 2.46, 95% CI 1.09–5.57; p = 0.03). Conclusions: Among STEMI patients undergoing primary PCI, contrast/GFR ratio ≥2.13 was independently associated with CI-AKI.
Staphylococcus aureus Carriage Status in Patients with Hidradenitis Suppurativa: An Observational Cohort Study in a Tertiary Referral Hospital in Athens, Greece
Authors: Katoulis Alexander , Koumaki Vasiliki , Efthymiou Ourania , Koumaki Dimitra , Dimitroulia Evangelia , Voudouri Maria , Voudouri Alexandra , Bozi Evangelia , Tsakris Athanasios
Published: December 2019

Background: Hidradenitis suppurativa (HS) is a chronic, recurrent, auto-inflammatory disease that affects the pilosebaceous unit in apocrine gland-bearing areas. Bacteria are thought to play a role in the development and progression of disease. In addition, antibiotics are frequently used as first-line management for HS. We sought to determine the carriage status of Staphylococcus aureus and its resistance to antibiotics among patients with HS in a tertiary referral hospital in Athens, Greece. Methods: In this observational cohort study, 68 consecutive patients attending the HS clinic of “Attikon” General University Hospital in Athens, Greece, during a 9-month period were enrolled. All patients had not received any antibiotic therapy for any reason during the previous 3 months before enrollment. Nasal and oropharyngeal samplingwere obtained, and specimens were tested for the presence of S. aureus.Antibiotic susceptibility testing was performed using the VITEK 2 system. Standard statistical tests, descriptive statistics tests, and χ2 and Pearson correlation tests were performed, using IBM SPSS Statistics 25.The level of significance was set at a pvalue <0.05. Results: Sixty-eight patients with HS were studied. There were 44 females (64.7%) and 24 males (35.3%). The mean age was 36.63 ± 13.0 (IQR = 21), and the mean age at onset of disease was 23.90 ± 11.53 (IQR = 14). The mean duration of disease was 12.74 ± 10.20 years (IQR = 15). Fifteen (22.1%) of the patients were Hurley stage I, 22 (32.4%) were Hurley stage II, and 31 (45.6%) were Hurley stage III. S. aureus carriage was detected in 17 patients (25%). Six of them (35.3%) had MRSA strains. There was an increased prevalence of S. aureus colonization (p = 0.058) and MRSA (p = 0.101) in Hurley stage III patients, but this result was not statistically significant. Conclusions: We found a 25% prevalence of S. aureus colonization (17/68 patients) and a 35.3% prevalence of MRSA (6/17) among our HS patients. There was an increased prevalence of S. aureusand MRSA positivity in HS patients with Hurley stage III. Further studies are needed to clarify the possible clinical significance of S. aureus carriage in the disease development and progression as well as in the treatment outcome.
Management of Gastroesophageal Reflux Disease in Asian Countries: Results of a Questionnaire Survey
Authors: Hojo Mariko , Nagahara Akihito , Hahm Ki-Baik , Iwakiri Ryuichi , Watanabe Toshio , Rani Abdul Aziz , Zhu Qi , Chan Francis K.L. , Sollano Jose D. , Kamiya Takeshi , Yamaguchi Satoru , Motoya Satoshi , Fock Kwong Ming , Fukudo Shin , Kachintorn Udom , Suzuki Hidekazu , Murakami Kazunari ,   The International Gastroenterology Consensus Symposium Study Group
Published: December 2019

Introduction: The Asia-Pacific consensus on the management of gastroesophageal reflux disease (GERD) and the GERD treatment guidelines of 2015 drawn up by the Japanese Society of Gastroenterology were proposed, and GERD management in Asian regions was assumed to be performed based on these consensuses. In this environment, the current status of GERD management in clinical practice among Asian regions is less well-known. Objective: This questionnaire-based consensus survey was performed to clarify the current status of management of GERD in clinical practice in Asian regions. Methods: A questionnaire related to management of GERD was distributed to members of the International Gastroenterology Consensus Symposium Study Group. We analyzed the questionnaire responses and compared the results among groups. Results: The frequencies of erosive GERD (ERD), non-ERD, uninvestigated GERD, and Barrett’s esophagus varied significantly among Asian countries. The most important factor in diagnosing GERD was the presence of symptoms in all countries. A proton pump inhibitor was the most commonly prescribed drug to treat GERD in all countries. Endoscopic surveillance for GERD was performed regularly. Conclusion: This questionnaire survey revealed the current status of management of GERD in clinical practice in various Asian countries.