bluuebunny/crossref_metadata_2025 huggingface dataset:
To determine the association of serum AMH levels with oocyte quality in infertile women who underwent controlled ovarian stimulation (COS) for IVF cycles
Authors: Daruwale Rani Vijay
Published: April 2022

There is well documented evidence showing a decline in female fertility with advanced age. This decline includes a significant reduction in the quantity as well as the quality of the oocyte available in the ovarian reserve. The present study was conducted to determine the association of serum AMH levels with oocyte quality in infertile women who underwent controlled ovarian stimulation (COS) for IVF cycles. The present retrospective study conducted in IVF Department of Nowrosjee Wadia Maternity Hospital for 10 months. Total 100 patients were included in the study.  Serum AMH levels and number of antral follicle (on second day of menstrual cycle) on transvaginal sonography was done for all patients. Fertilization was checked followed by embryo quality. Statistical analysis was performed by ANOVA. Significance was attributed to events with a p<0.05. A Total 100 patients were studied. The patients were divided into five groups based on AMH levels; the groups had 0, 44, 20, 26 and 10 patients respectively. Age was found to be independent predictor of ovarian reserve. There was no significant difference between the groups in relation to BMI.
Odgoj, obrazovanje i formacija za ekumenski i međureligijski dijalog prema dokumentima Katoličke crkve
Authors: Zečević Božić Juro , Kovač Tomislav
Published: April 2022

Dokumenti i izjave učiteljstva Katoličke crkve, osobito nakon Drugoga vatikanskog koncila, naglašeno ističu ekumensku i međureligijsku dimenziju u afirmativnim pa i obvezujućim kontekstima. Potreba odgoja, obrazovanja i formacije za ekumenski i međureligijski dijalog nedvojbeno je prepoznata i priznata na deklarativnoj razini, no na provedbenoj razini stanje se razlikuje ovisno o različitim, povoljnim i manje povoljnim, okolnostima pojedinih sredina i socioloških okružja u svijetu. Cilj ovog rada jest tu činjenicu zauzimanja crkvenog učiteljstva da novi naraštaji u Crkvi budu odgojeni, obrazovani i formirani, dakle osposobljeni za konstruktivne odnose i suživot u realno egzistentnom pluralnom svjetskom društvu, s mnoštvom različitih konfesija, religija i svjetonazora, potkrijepiti i ilustrirati konkretnim izjavama crkvenog učiteljstva različitih razina, u relevantnijim dokumentima Katoličke crkve te time pridonijeti posvješćenju tih načela i na provedbenoj razini i većem stupnju usklađenosti institucionalnih komponenti i svih vjernika s tim smjernicama, poticajima i očekivanjima učiteljstva Crkve. U prvom dijelu obrađuju se dokumenti vezani uz odgoj, obrazovanje i formaciju za ekumenizam, a u drugom dijelu, dokumenti koji su pod tim vidom vezani za međureligijski dijalog.
Socijalni dijalog 2030.
Authors: Baturina Danijel , Bežovan Gojko , Matančević Jelena
Published: April 2022

Cilj rada je raspraviti potencijalne pravce razvoja socijalnog dijaloga u Hrvatskoj. Rad prikazuje rezultate istraživanja provedenog krajem 2019. godine s ciljem dobivanja uvida u perspektive socijalnog dijaloga u okolnostima promijenjenih industrijskih odnosa u Republici Hrvatskoj u 2030. godini. Istraživanje je provedeno delphi-metodom s osam stručnjaka, predstavnika ključnih dionika socijalnog dijaloga: vlade, sindikata i poslodavaca te istraživača iz područja industrijskih odnosa. Rezultati su raspravljeni u kontekstu očekivanih ekonomskih, društvenih i političkih faktora koji će utjecati na promjene socijalnog dijaloga do 2030. godine. Naglašeno je nekoliko glavnih istraživačkih nalaza. Kvaliteta i spremnost na socijalni dijalog u Hrvatskoj je relativno niska, a institucionalni okvir industrijskih odnosa oslabljen te se ne očekuju značajna poboljšanja do 2030. godine. Sindikati će se naći pred izazovom zadržavanja relevantnosti u promijenjenim socioekonomskim okolnostima poput nedostatka radne snage i promjena u obrascima zaposlenosti te tehnoloških promjena. Očekuje se nastavak trendova decentralizacije pregovaranja, odnosno jačanja bipartitnog socijalnog dijaloga na razini poduzeća. Ističe se potreba za inovativnijim djelovanjem sindikata te za razvijanjem novih strategija u privlačenju mlađih članova.
Exploring First Nation-held Forest Tenures and Community Forest Enterprises in British Columbia
Authors: Timko Joleen A. , Pizzirani Stefania , Kozak Robert A. , Bull Gary
Published: April 2022

The purpose of this report is to situate existing knowledge on First Nation-held forestry tenures and community forest enterprises (CFEs) in British Columbia, Canada within a broader discussion about Indigenous and non-Indigenous community forests in Canada. This report provides 1) A brief characterization of Indigenous forestry partnerships across Canada; 2) A description of the two most common First Nation-held forest tenures within British Columbia: the First Nations Woodland License and the community forest agreement; 3) An assessment of challenges and constraints facing First Nation-led CFEs in British Columbia; 4) An assessment of key enabling conditions in First Nation-led CFEs in British Columbia; and 5) Recommendations to enable Indigenous communities, policymakers, the private sector, and supporting institutions to strengthen the business proposition of Indigenous-led CFEs in British Columbia and elsewhere in Canada.
From Risk and Conflict to Peace and Prosperity: The urgency of securing community land rights in a turbulent world
Authors:   Rights and Resources Initiative
Published: April 2022

Amid the realities of major political turbulence, there was growing recognition in 2016 that the land rights of Indigenous Peoples and local communities are key to ensuring peace and prosperity, economic development, sound investment, and climate change mitigation and adaptation. Despite equivocation by governments, a critical mass of influential investors and companies now recognize the market rationale for respecting community land rights. There is also increased recognition that ignoring these rights carries significant financial and reputational risks; causes conflict with local peoples; and almost always fails to deliver on development promises.
Tenure and Investment in Southern Africa: Sugar, Mining, Monitoring and Expectations
Authors:   Rights and Resources Initiative,   TMP Systems
Published: April 2022

Tenure disputes in Southern Africa have created financial and reputational damage for the companies and investors involved. Sectors like sugar and mining have struggled to engage local peoples effectively and to manage local expectations associated with their projects. Disputes in Southern Africa are particularly likely to lead to materially significant events, like work stoppages. They are also more likely to be violent than in any other region in the world, which is a considerable deterrent for foreign direct investment (FDI). This paper examines recent case studies of tenure-related dispute in Southern Africa to help companies, investors, governments, and CSOs to avoid and resolve them more effectively.
Tenure and Investment in East Africa: Power and Bioenergy
Authors:   Rights and Resources Initiative,   TMP Systems
Published: April 2022

Tenure disputes in East Africa have created financial and reputational problems for the companies and investors involved. These issues are becoming significant at a macro-level as well as a project level. Energy projects have experienced contagious disputes that have undermined the attractiveness of the sector for international investors in key countries. This paper examines recent case studies of tenure-related dispute in East Africa to help companies, investors, governments, and CSOs avoid and resolve them more effectively.
PROFESSIONAL COMPETENCES OF PHYSICAL EDUCATION TEACHERS/ՖԻԶԻԿԱԿԱՆ ԿՈՒԼՏՈՒՐԱՅԻ ՈՒՍՈՒՑԻՉՆԵՐԻ ՄԱՍՆԱԳԻՏԱԿԱՆ ԿՈՄՊԵՏԵՆՏՈՒԹՅՈՒՆՆԵՐԸ
Authors: Papikyan A.
Published: April 2022

The article presents the professional competences of physical education teachers. Vocational training will be effective only if it includes a knowledge system, basic means of activity, and the formation of a worldview, which will further form the components of the competencies of the future specialist./Հոդվածում ներկայացված են ֆիզիկական կուլտուրայի ուսուցիչների մասնագիտական կոմպետենտությունները (կարողունա-կությունները): Պրոֆեսիոնալ պատրաստությունը արդյունավետ կլինի միայն այն ժամանակ, երբ կներառի գիտելիքների համակարգ, գործունեության հիմնական միջոցներ, աշխարհայացքի ձևավորում, որոնք հետագայում կկազմեն ապագա մասնագետի կոմպետենցիաների բաղադրիչները:
Vicia Lathyroides L. (Fabaceae) - A New Record for the Flora of India
Authors: Bhellum B.
Published: April 2022

The present paper put on record Vicia lathyroides L. (Fabaceae) a species new to the flora of India. The species was collected from Jammu region, Jammu and Kashmir State in 2010. Since it has not been reported earlier from this region therefore, recorded an addition to the flora of India.
Ecological status of Wild Edibles consumed by Cholanaickens, a primitive tribe of Western Ghats, India
Authors: Ashraf K. , Santhoshkumar A.
Published: April 2022

A study was undertaken to document the ecological status of wild edibles consumed among the Cholanaickens tribe of Western Ghat in India. It was found that they consume a number of wild edible plants. It was observed that the people are less dependent on their traditional foods owing to the availability of subsidized food from the cooperative stores. A total of fifty six species of wild edible plants were documented which are consumed by the Cholanaickens. Among those species, twenty four species were leafy vegetables, followed by twelve fruit yielding species, nine species, which produce edible seeds, and eleven species producing tubers and rhizomes. The phytosociological study showed that only thirteen species of edible plants are available at present in the study area. It was also found that regeneration of most of the tuber species is not taking place probably because of higher exploitation in the previous period.
A MÚSICA COMO RECURSO PEDAGÓGICO NO PROCESSO DE ALFABETIZAÇÃO DE CRIANÇAS COM NECESSIDADES ESPECIAIS
Authors: de Fátima Barboza Ferreira Sandra , dos Santos Borges Gabriel , do Rocio Guadagnini Míriam , Yamauchi Torres Érica , Santos Gonçalves Barreto Bezerra Cláudia
Published: April 2022

No contexto da inclusão escolar as pesquisas vêm indicando que metodologias multissensoriais são mais efetivas. A música pode ser utilizada neste contexto explorando principalmente aspectos culturais e motivacionais e linguísticos que envolvam aspectos fonético-fonológicos. O objetivo deste estudo é selecionar um conjunto de músicas com conteúdo explícito de alfabetização com vistas a prover uma base que subsidie o professor na abordagem intencional destes elementos. Trata-se de uma pesquisa exploratória, descritiva e qualitativa. Procedeu-se a uma busca na web utilizando-se o descritor música e alfabetização e como critério de inclusão o ensino explícito da leitura/escrita. Selecionaram-se dez músicas no intuito de estabelecer categorias pós-fixadas de análise organizando o conteúdo a partir de sua vinculação direta com o desenvolvimento da habilidade de leitura e escrita. Os resultados apontaram para elementos relacionados à representação pictórica, leitura labial/gestual, consciência fonético-fonológica, segmentação da palavra e conversão fonema/grafema. Discutiu-se a importância da intencionalidade e apresentação explícita do conteúdo. A música constitui um recurso acessível em duplo sentido, em relação à sua disponibilidade e por integrar vários aspectos pragmáticos e linguísticos, afetivos. Palavras-chave: Alfabetização; letramento; música; inclusão escolar
RECURRENT NEURAL NETWORK BASED MODEL DEVELOPMENT FOR WHEAT YIELD FORECASTING
Authors: ÇETİNER Halit , KARA Burhan
Published: April 2022

In the study carried out in line with the stated purposes, monthly rain, humidity and temperature data, wheat production amount, and wheat productivity data of Konya province between 1980-2020 were used. Using these data, wheat productivity estimation was performed with (Gated Recurrent Units) GRU and Long Short Term Memory (LSTM) methods, which are Recurrent Neural Network (RNN) based algorithms. When wheat productivity estimation performance was examined with the implemented GRU-based model, 0.9550, 0.0059, 0.0280, 0.0623, 7.45 values were obtained for the R2 score, MSE, RMSE, MAE and MAPE values, respectively. In the performance results obtained with the LSTM method, which is another RNN-based method, 0.9667, 0.0054, 0.0280, 0.0614, 7.33 values were obtained for the R2 score, MSE, RMSE, MAE and MAPE values, respectively. Although the LSTM method gave better results than the GRU method, the training modelling time of the LSTM method took longer than that of the GRU method.
A Conversation About Climate Change In Somalia
Authors: Wehliye Fardowsa , Glaser Sarah
Published: April 2022

There is a striking inequality when it comes to the effects of climate change on more and less developed nations. Developed nations fail to fully address their issues while developing nations show how climate change can impact the individuals in that nation. In underdeveloped countries like Somalia, there is a lack of education and awareness on the topic. To increase awareness and understanding media and tv can play a large role in education but it is necessary for all of us to be a part of the solution. Currently, there are strong storms, cyclones, and monsoons in Somalia creating a decline in fishing resources now showing that the effects of climate change are becoming more noticeable. supporting green energy solutions can be very powerful to help counteract climate change in Somalia. To stop climate change there needs to be more education on the topic and everyone has to work together to help end climate change.
Climate Resilience: It Is About Time!
Authors: Belhabib Dyhia
Published: April 2022

In 2006 the New York times, there were many articles about the struggles that were happening in Somalia and Ethiopia due to climate change. At this time 300,000 Algerians lost their lives to terror this plays into climate change due to the common feature of extremist groups being born as a byproduct of popular frustration in this case it was resource scarcity. At that time we didn't think much of these issues and did not link these issues back to climate change. This edition of Poplar & Ivy will explore the impact of climate change and strategies for resilience against it.
MISCONCEPTION INTERVENTIONS AND PUPILS’ CONCEPTUAL CHANGE IN SELECTED CONCEPTS IN BASIC SCIENCE IN ABEOKUTA
Authors: OMILANI Nathaniel Ayodeji , ANIFOWOSE Hikmot
Published: April 2022

This study determined the effect of misconceptions intervention on students’ conceptual change in the concepts of density and floatation in basic science. The moderating effects of gender and school types on students conceptual change in the selected concepts were also determined. The misconception interventions in this study are hands-on activities that allow students who engage in them to observe evidence that contradict their misconceptions in density and floatation. Students in the upper basic three were the population of the study. A two-tier multiple-choice test was used to measure the conceptual change. The result revealed that exposing upper basic three students to the misconception intervention is significantly effective in facilitating conceptual change in the selected concept in basic science.
UMA CIDADE ENTRE NARRATIVAS: CONSTRUÇÕES HISTÓRICAS SOBRE A HISTÓRIA DE ORLÂNDIA-SP
Authors: Pereira Bruno César
Published: April 2022

O presente artigo, propõe realizar uma análise de duas narrativas que constroem a história do município de Orlândia, localizado no noroeste do Estado de São Paulo. Em um primeiro momento, analisaremos uma narrativa construída através de importantes memorialistas do município, a qual é compreendida enquanto “oficial” e se encontra preservada nos espaços de memória da cidade (Biblioteca, Casa da Cultura e Museu). Em sequência, analisaremos uma outra narrativa, na qual pode ser compreendida enquanto “marginal”, desenvolvida através de crônicas, que vai na contramão da Orlândia idealizada pelos memorialistas. Neste sentido, buscaremos, ao longo deste texto, compreender estas “duas Orlândias”, de um lado uma cidade enquanto um “moderno de projeto urbanístico”, e de outro, a cidade de “contradições”, marcada pela desigualdade de seus espaços e pela estigmatização de seus moradores. Contudo, como destacaremos, estas narrativas podem ser compreendidas enquanto um conjunto, pois cada uma destas se refere a um espaço e não ao todo.
تقييم بيانات الوفيات وتبيين دورها في الانتقال الديمغرافي في الجزائر
Authors: مرداسي نجاة
Published: April 2022

تتأثر الظواهر السكانية في المجتمعات النامية مثل الجزائر بشكل كبير ومباشر بالتغيرات التي تطرأ على مختلف الأصعدة الاقتصادية والاجتماعية والثقافية وحتى السياسية، ودراسة أي ظاهرة منها كالوفيات يقتضي التطرق إلى جانبين أساسيين هما تقييم جودة البيانات ومدى توفرها وذلك من أجل إجراء دراسات دقيقة وموسعة تسمح بالوصول إلى نتائج علمية؛ خاصة مع توفر تقنيات التقدير غير المباشر الحديثة والمتوفرة للجميع.
تتباين آراء الباحثين حول مسار الانتقال الديمغرافي في الجزائر فمنهم من يرى أنها في نهاية المرحلة الثانية وهناك من يرى أننا في المرحلة الثالثة، غير أن الجميع متفق على أن هذا التطور غير منتظم تماما بفعل تأثير مختلف الظروف التي مرت بها الجزائر خاصة منذ منتصف ثمانينات القرن الماضي.
وجدنا أن مسار الوفيات هو نفسه المتعارف عليه عند تحديد مراحل الانتقال الديمغرافي في الجزائر، غير أن بياناتها لا تخلوا من بعض العيوب، إضافة إلى أن أزمة عشرية التسعين أثرت مؤقتا على مسار تغير الوفيات وتزامنت مع بداية مرحلة جديدة.
الاكتساب اللغوي و تعدّد أصوله الإيديولوجية والمعرفيّة
Authors: قانة أمال
Published: April 2022

تعدّ عمليّة اكتساب اللّغة من أهم القضايا التي استقطبت اهتمام المفكّرين والباحثين في مجال علم النّفس التربوي، وقد فتح هذا الموضوع آفاقًا هائلة أمام البحث العلمي، ذلك أنّ الاكتساب اللغوي يحدث في الطّفولة، فهو سلوك متطوّر ونمائي، وعليه تم تسليط الضوء في بحثنا عن تطوّر النمو اللغوي عند الطّفل بكل حيثيّاته هذا من جهة، ومن جهة أخرى البحث في قضية تعدّد الأصول المعرفية والفلسفية التي فسّرت الاكتساب اللغوي، والحديث عنها يقتضي بالضرورة التطرّق للنظريات، والتّفصيل فيها لمعرفة المرجعيات الإيديولوجية التي تأسّست عليها، وقد أسفرت النتائج عن وجود اختلاف في الرٌّؤى والتّصوّرات المعرفية، فالنظرية السلوكيّة مثلاَ تفسّر اللّغة كشكل من أشكال السلوك؛ في حين النّظرية التوليدية التحويلية ركّزت على المهارات العقلية، وقدرة الطّفل الإبداعيّة على إنتاج الجمل النّحوية، أمّا النّظرية المعرفيّة قدّمت طرحًا مغايرًا يقوم أساسًا على التّطوّر المعرفي...
العلمانية في الفكر العربي المعاصر بين القبول والرفض - محمد أركون وطه عبد الرحمان أنموذجا -
Authors: الألي الرافع , بونمورة جعفر , مرمي سهام
Published: April 2022

استثارت العلمانية، كونها شديدة الصلة ببناء الأطر الاجتماعية  لدى المفكرين استشكالات راهنة  كشفت من خلالها الرؤى التي ألت إليها المجتمعات.
وبالطرح التساؤلي : هل الممارسات العلمانية ،أحدثت فعل التقدم واستمراريته في المجتمعات أم لا؟
وعليه جاءت هده الدراسة بهدف بيان المصد من العلمانية، وتوضيح الدور الذي تقوم به هل ينحصر في مبدأ تحديث المجتمعات، كما أقر به محمد أركون؟ أم ينحصر في مبدأ أن أفق العلمانية عقيم؟ وبالتالي طرح بديل كرؤية استشرافية  تخلص المجتمع العربي خاصة، من مخلفات العلمانية كما أوضحها المفكر  طه عبد الرحمان ضمن مشروعية الائتماني.
          ومن ثمة الوقوف على مشروعية النتائج المحققة إزاء تطبيق العلمانية، في المجتمعات الغربية والعربية على حد سواء.
دور المستنبتات المخدرة في تهديد الامن المجتمعي -دراسة ميدانية اجتماعية قانونية في فلسطين
Authors: ابوعواد رؤوف
Published: April 2022

الملخص:
هدفت الدراسة إلى التعرف على دور المستنبتات المخدرة في تهديد الأمن المجتمعي في محافظة الخليل من وجهة نظر العاملين في قسم مكافحة المخدرات في الشرطة، استخدم الباحث في هذه الدراسة المنهج الوصفي التحليلي، تكون مجتمع الدراسة من العاملين في قسم مكافحة المخدرات في جهاز الشرطة في محافظة الخليل، حيث تكونت العينة من 57 فرداً، وقد تم استخدام الاستبانة لتحقيق أهداف الدراسة وتوصلت الدراسة إلى أن دور المستنبتات المخدرة في تهديد الأمن المجتمعي في محافظة الخليل من وجهة نظر العاملين في قسم مكافحة المخدرات كان مرتفعاً، كما تبين أنه لا توجد فروق في دور المستنبتات المخدرة في تهديد الأمن المجتمعي في محافظة الخليل من وجهة نظر العاملين في قسم مكافحة المخدرات في الشرطة تعزى إلى متغير الجنس، ومكان السكن والمستوى التعليمي، كما أظهرت الدراسة أنه توجد فروق في دور المستنبتات المخدرة في تهديد الأمن المجتمعي في محافظة الخليل من وجهة نظر العاملين في قسم مكافحة المخدرات في الشرطة التي تعزى إلى متغير سنوات الخبرة، وحيث أن الفروق كانت بين ذوي الخبرة أقل من 5 سنوات وبين ذوي الخبرة 5-10 سنوات وكانت الفروق لصالح ذوي الخبرة 5-10 سنوات الذي كان المتوسط الحسابي عندهم أعلى. وأن الفروق كانت لصالح ذوي الخبرة  اكثر من 10 سنوات، بناءً على ذلك يوصي الباحثان أن يتم التركيز على موضوع المخدرات من خلال بث برامج التوعية واستهداف الشباب خوفاً من انجرارهم نحو هذه الآفة، وأن يتم التعاون بين وسائل الإعلام والتربية والتعليم من أجل عقد جلسات يتم بثها مباشرة حول قضايا المخدرات. وأن تعزز وسائل الإعلام برامجها الموجهة نحو علاج مشكلة المخدرات وخصوصاً نحو الأسرة لتوجيههم نحو التعامل السليم مع هذه الظاهرة.
Prospects for Attracting Islamic Financial Industry Institutions to Algeria after 2024 - Requirements and Challenges
Authors: Boualam Oualhi
Published: April 2022

This study aimed to identify the opportunities and possibilities available for nationalizing the Islamic financial industry in Algeria at present and after 2024, in light of the growing international economic challenges and the increasing requirements of sustainable development. The problem of this study revolves around the prospects for attracting Islamic financial institutions to Algeria after 2024 in terms of requirements and challenges. It is divided into four themes: the milestones of Islamic banking under the new Law No. 20-02, the reasons for integrating Islamic financial institutions into the financing process, the expected opportunities for the assets of the Islamic finance industry in Algeria Horizons 2040, and the challenges of implementing financial institutions in Algeria. The study concluded that there is a political will on the part of the public authorities in Algeria that has culminated in the issuance of the Islamic banking law as an alternative to the participatory banking system. This is expected to be a strategic option for mobilizing the hoarded financial resources of families and those circulating in the informal market that finances the shadow economy, especially if various challenges are faced. The most important of these are legislative challenges, challenges of financial inclusion, and challenges related to administrative and financial corruption.
APPLICATION OF BACTERIOCIN-PRODUCING LACTIC ACID BACTERIA ISOLATED FROM SALTED DRIED CAMEL MEAT (EL KADID) IN THE PRESERVATION OF GOAT CHEESE AGAINST Staphylococcus aureus
Authors: Bouchefra Amina , Montanari Chiara , Barbieri Federica , Khennouf Tarek , Gardini Fausto , Idoui Tayeb
Published: April 2022

El kadid is a traditional North African food obtained by natural dehydration of One-humped camels (Camelus dromedarius) meat still produced through traditional method in Algeria. This traditional food matrix constitutes a rich ecological niche for the screening of bacteriocin-producing strains and lactic acid bacteria which contributes to the safety and preservation of foods. Fifteen lactic acid bacterial isolates from El kadid were tested for antibacterial activity and biopreservative effectiveness against Staphylococcus aureus. Only four strains exhibited bacteriocin activity against the two indicator bacteria, Listeria monocytogenes ATCC 4379G and Staphylococcus aureus ATCC 25923, in the neutralized cell-free supematant treated with catalase and proteolytic enzymes. Strain LB6, identified as Lactobacillus plantarum and characterized by the highest antimicrobial activity, was selected for further trials. Bacteriocin rate peaked during the stationary phase. The ideal temperature and pH for its formation were 37°C and 6.5, respectively. The bacteriocin was stable at high temperatures (30 min at 100°C) and active following incubation at pH levels ranging from 2 to 12. This strain showed high activity in fresh goat cheese (11–15 mm inhibition zone) having the ability to prevent the development of S. aureus, also reducing the inoculum level of pathogen by about 1 log cycle. These results revealed that this strain could be a promising biopreservative starter for the dairy industry. To the author ’s knowledge, this is the first report of L. plantarum being isolated in salted dried camel meat.
PHYSICOCHEMICAL AND SENSORY CHARACTERISTICS OF KASHK AS INFLUENCED BY QUINOA FLOUR ADDITION
Authors: Sekhavatizadeh Seyed Saeed , Mirzaee Najmeh , Golmakani Mohammad-Taghi , Hosseinzadeh Saeid
Published: April 2022

The current work was aimed to study the application of quinoa flour in the production of kashk, fermented dairy product and the resulting physicochemical and sensory properties. Three kashk samples were formulated with quinoa flour (1,2 and 3)%. Pretreatment of the kashk samples with quinoa flour had significantly increased the viscosity (from 4040 to 4127 cP), acidity value (increased 0.3) and reduced the syneresis of the samples (P < 0.05). Concerning color, no significant changes were observed in the “b” and “L” values in all the samples which remained constant following 60 days of storage whereas an increase in the “a” value was observed upon supplementation of quinoa flour (from -3.00 ± 0.50 to -0.11 ± 0.51). Sensory analysis revealed that increasing the quinoa concentration increased consistency but decreased flavor. Scanning electron micrographs revealed that the denser microstructure was seen in kashk containing quinoa flour. This could suggest that quinoa flour could be used to improve the microstructure of the kashk. Moreover, adding quinoa flour to kashk could compensate lysine depleted in the kashk thermal process. These results indicated that kashk supplementation with quinoa flour could be suggested as an effective means to improve the functional properties of kashk.
ISOLATION, SCREENING, AND PARTIAL CHARACTERIZATION OF OXALATE OXIDASE ENZYME FROM PSEUDOMONAS STRAIN UNDER INDUCED OXALATE STRESS CONDITION
Authors: PATIL ANITA , PAIKRAO HARIPRASAD , SHEIKH RUBINA , DHAKATE LEENA
Published: April 2022

Oxalate oxidase (OxO), a manganese dependent enzyme, is involved in the catalysis of oxalate oxidation to carbon dioxide with the formation of hydrogen peroxide. OxO is present in the cell wall of plants. It increases the resistivity against diseases and external stress. Oxalate is found as waste metabolites in mammals, excess accumulation of oxalate results in hyperoxaluria and urolithiasis in humans. These disorders can be diagnosed by the help of OxO in many analytical methods. The present study is aimed on isolation of OxO enzyme from  Pseudomonas strain under high salt stress. The OxO enzyme was produced in bulk under selected salt stress. It was partially purified by ammonium sulphate precipitation method, dialysis and ion-exchange chromatography. The OxO enzyme on enzymatic analysis showed potent activity concerning oxalic acid substrate. The microtiter plate analysis confirmed the ability of OxO enzyme purified from Pseudomonas strain in the diagnosis of oxalate-related disorders and in the future could be used to prepare the diagnostic biosensors.
NOVEL RUMMELIIBACILLUS SP. ISOLATED FROM FERMENTED VEGETABLE PRODUCTS AS THE POTENTIAL PROBIOTICS
Authors: Tinrat Sirikhwan , Sedtananun Saranya
Published: April 2022

The novel lactic acid bacteria has potential probiotics which were selectively screened from different fermented vegetable products by antagonistic activity against Escherichia coli ATCC 25922 as a food-borne pathogenic bacteria. From 40 isolates on MRS agar, some isolates had anti-E. coli activity. Surprisingly, isolated STR 0103f and STR 0404f strains demonstrated bile salt-tolerance at 0.3% bile salt for 24 hours with survival rate of 67.77±0.38 and 68.72±0.45%, respectively. Both strains also survived under acidic conditions (pH 3-5) with 60.53±0.08-76.87±0.09% of survival rate. STR 0103f and STR 0404f strains elicited the broad spectrum against both Gram-negative (Pseudomonas aeruginosa ATCC 27853, Salmonella typhimurium ATCC 13311 and Salmonella  paratyphi A) and Gram-positive (StaphylococcusS. aureus ATCC 25923) pathogenic strains which appeared inhibition zone of 7.33±0.58-8.33±0.58 mm and revealed a susceptibility to various antibiotics. Moreover, two selected strains were also able to adhere to stainless steel plates at least 30% of adhesion rate and without any detectable hemolytic activity. Morphological, biochemical characteristics and 16S rRNA gene sequences were used as a tool for bacterial identification. Both STR 0103f and STR 0404f isolates were identified as Rummeliibacillus sp. which are Rummeliibacillus pycnus STR 0103f and Rummeliibacillus stabekisii STR 0404f, respectively. Thus, R. pycnus STR 0103f and R. stabekisii STR 0404f would be the novel bacterial strains from fermented vegetable products that act as the potential probiotic candidates for safety food bio-preservation.
INFLUENCE OF HYDRODYNAMIC PARAMETERS ON THE SYNTHESIS OF TARGET METABOLITES AND THE DEGREE OF DISINTEGRATION DURING THE SUBMERGED CULTIVATION OF MICROMYCETES
Authors: Motronenko Valentyna , Bakalchuk Mariіa , Novosad Andrii , Harmash Oksana , Marynchenko Lolita
Published: April 2022

One of the limiting parameters of the mycelial fungi submerged cultivation for the synthesis of metabolites used for medical, food and industrial purposes is the hydrodynamic conditions of submerged cultivation.
The effect of mechanical stirring rate on the yield of metabolites was investigated on the example of cultivation of mycelial fungi Aspergillus awamori – glucoamylase producer. It was found that the maximum yield of the enzyme was observed at the stirring speed of turbine impeller amounting to 60-120 rpm. As the stirring speed increased, the amount of synthesized metabolite decreased, which is a consequence of the destruction of the mycelial cells. The connection between the decrease in glucoamylase synthesis and the rejuvenation of the culture caused by its recovery from fragments of the destroyed mycelium and the subsequent increase in the amount of biomass was shown. At the same time, the degree of destruction was shown by the increase in the amount of nucleic acids in the filtrate.
The relationship between the degree of destruction of mycelial cells, determined by the amount of nucleic acids in the filtrate under extreme conditions of ultrasonic disintegration, and the viability of the culture was also demonstrated. The amount of viable cells increased at first (until the saturation of the culture fluid with nucleic acids), and then began to gradually decrease. The obtained results can serve as a starting point for further studies of the possibility of stimulating the primary accumulation of biomass of micromycetes by ultrasound.
DIRECT RECOMBINASE POLYMERASE AMPLIFICATION ASSAY FOR ACCURATE AND RAPID DETECTION OF LISTERIA MONOCYTOGENES IN FOOD
Authors: Tran Hau Thi , Tran Diem Hong , Pham Trang Nguyen Minh , Phung Huong Thi Thu
Published: April 2022

Listeria monocytogenes is one of the most common types of food poisoning bacteria which can cause serious foodborne diseases or even lethality. Generally, L. monocytogenes can be detected using traditional microbiology or molecular biology techniques, notably PCR. However, the application of these methods at the field is restricted due to the strict requirement of equipment and skilled personnel. In this study, recombinase polymerase amplification (RPA), an isothermal PCR assay was developed to rapidly detect L. monocytogenes in crude samples. The results showed that the RPA reaction, without requiring complex thermal cycles, was well-performed in the optimal conditions of 39°C within only 25 minutes. The limit of detection was identified as 310 fg of L. monocytogenes genomic DNA, which was 1000-fold more sensitive than the conventional PCR. RPA also succeeded to directly detect L. monocytogenes cells at a concentration as low as 2.5 × 101 Colony Forming Unit (CFU)/mL in pure cultures. In addition, RPA could accurately detect L. monocytogenes at 2.5 × 102 CFU/mL in milk without sample extraction or processing. Therefore, RPA established in this study could be an alternative standard method to confirm the presence of L. monocytogenes in food. Accordingly, this rapid and sensitive method could be further applied to clinical testing for the diagnosis of L. monocytogenes infection, especially in areas with limited settings.
GENOTYPIC AND PHENOTYPIC CHARACTERIZATION OF BACTERIOCINOGENIC LACTIC ACID BACTERIAL STRAINS FOR POSSIBLE BENEFICIAL, VIRULENCE, AND ANTIBIOTIC RESISTANCE TRAITS
Authors: Perez Rodney , Ancuelo Amily E. , Zendo Takeshi
Published: April 2022

Lactic acid bacteria (LAB) are a diverse group of microorganisms that can be found in natural habitats and are used in a wide range of industries, especially in the food sector. They are regarded "Generally Recognized as Safe" and are commonly used as a starter culture, probiotics, and biopreservative. However, some LAB genera are excluded from the "Qualified Presumption of Safety" (QPS) list due to the possibility that they have virulence and antibiotic resistance genetic determinants. The objective of this study was to investigate the beneficial, virulence, and antibiotic resistance-related genes in 10 well-characterized bacteriocinogenic LAB strains. The auto-aggregation, co-aggregation, cell surface hydrophobicity, and bile salt tolerance were also evaluated to examine their potential as bacteriocinogenic probiotics and/or starter culture. Results showed that all tested strains showed abilities for auto-aggregation at 4oC and 37oC, co-aggregation with S. aureus JCM8704, S. typhimurium BIOTECH1826, and E. coli DH5α, and significant cell-surface hydrophobicity. However, only a few strains were able to withstand the media treated with 0.3% bile salt. Among the tested bacteriocinogenic LAB strains, L. lactis IO-1 and C. divergens V41 had the maximum values for auto-aggregation at 4oC and 37oC, respectively. C. divergens V41 also exhibited the highest percentage for cell surface hydrophobicity. E. faecium NKR-5-3 showed the highest co-aggregation percentages with all indicator strains. Our findings also showed that the tested isolates presented distinct combinations of virulence-related genes. Only two of ten bacteriocinogenic LAB strains exhibited presence of multiple virulence genes. Lactococcus sp. QU12 was found to have a high frequency of beneficial and virulence genes, with 2 out of 7 genes present encoding beneficial factor and 11 out of 13 genes encoding virulence factor. Lactococcus sp. QU12 and L. lactis IO-1 were also positive for tetracycline resistance gene tetM and aminoglycoside resistance gene aphA-2, respectively, and transposon genes. Moreover, only a few LAB isolates tested positive for 2 out of 8 antibiotic resistance classes. Although few, the substantial danger of these genes being transferred and acquired cannot be overlooked as this could potentially cause serious health concerns in the future. These results suggest that despite the promising properties of bacteriocinogenic LAB, careful safety evaluation of these strains should be a prerequisite before using these in food systems.
FLUORESCEIN LABELLED ANNEXIN V STAINING LIMITATIONS FOR DETERMINATION OF BLASTODERMAL CELLS APOPTOSIS
Authors: Svoradova Andrea , Zmrhal Vladimir , Lichovnikova Martina , Slama Petr
Published: April 2022

It is known that blastoderm contains not only blastodermal cells (BCs) but also cellular “debris”. These particles include yolk granules and lipid droplets. Some authors used AnnexinV (AnV) staining technique for determination of cell apoptosis. However, AnV is a protein with high affinity to lipids. So the aim of our study was to demonstrate the AnV binding in the blastoderm and yolk cell suspensions. Cell populations were obtained from fertilized chicken eggs. The blastoderms were isolated using filter paper ring method and mechanically fragmented. After that, samples were stained with AnV-FITC and analyzed by fluorescent microscopy (FM) and flow cytometry (FC). FM showed non-specific binding of AnV-FITC on the yolk and lipid particles. Therefore, the FC method using specific nuclear dye DRAQ5 was used to separate BCs from cellular debris. Only 1.82 ± 0.27 % cells were DRAQ5 positive. On the other hand, increase of total events (21 ± 3.30 %) of AnV positive cells without DRAQ5 dye was recorded. Moreover, the affinity of yolk to AnV by FC was also observed (10.45 ± 2.59 %). Significant differences among the groups (P < 0.001) were recorded. This trend was caused by non-specific binding of AnV to the lipid and yolk particles. So it is necessary to stain suspension with nuclear dye to separate BCs than co-stained with some marker of apoptosis. Based on the results we can summarized that AnV staining is inappropriate technique for apoptosis of BCs, however, using nuclear dye we can prevent of non-specific binding.
NEUROPROTECTIVE EFFECT OF VANDA TESSELLATA AS “RASNA” SPECIES, ON ALUMINIUM CHLORIDE INDUCED ALZHEIMER’S IN RATS
Authors: Raju Asirvatham , Salwa Abdul Salam , Daisy Punnackal Augustine , Aparna Ann Mathew , Boby Johns
Published: April 2022

The present study focused on evaluation of neuroprotective effect of hydromethanolic extracts of Vanda tessellate (VT), also considered as Rasna. Aluminum Chloride (AlCl3) induces neuroinflammation in rats and finally the development of AD. PASS online and molecular docking insilico studies were conducted with PPAR-γ for β-sitosterol and AChE for gigantiol. Total 36 trained Wistar rats were divided into VI groups 6 in each. Group I - normal control, Group II – Disease control, Group III – Rivastigmine (0.3mg/kg, p.o), Group IV and V – Hydromethanolic extract of VT (HMEVT, 150mg/kg, 300mg/kg, p.o) respectively, Group VI - Ayurvedic Formulation of Rasna (AFR) (1ml/kg, p.o). All the animals received Aluminum Chloride (AlCl3) (300mg/kg, p.o) except group I. The rats were treated for 20 days but mean time behavioural study, body weight changes were monitored on 0th, 7th, 14th& 20th day. On 21th day, rats were sacrificed, brains were isolated, then antioxidant enzymes levels, protein content and neurotransmitters levels were determined. Histopathology of cortex and hippocampus  parts of the brain were studied. Group II animals showed reduction in locomotor activity, increased in the number of entries as well as time spent in closed arm and time taken to climb the pole was increased but it was reversed in groups treated with 150mg/kg, 300mg/kg doses of HMEVT and AFR. Increased level of protein content, malondialdehyde, reduction in body weight and antioxidants enzymes like superoxide dismutase, catalase, glutathione were observed in disease control group and it was due to free radicals generation and were corrected and restored in groups treated with HMEVT and AFR. Moreover, the histopathological report also showed cellular level protection efficacy found with HMEVT and AFR. The neuroprotective action of HMEVT was due to the active constituents and was proved in insilico study. The order of neuroprotective efficacy was HMEVT > AFR.
Redo pull through combined procedures in Hirschprung disease
Authors: Novozhilov V. A. , Stepanova N. M. , Milyukova L. P. , Petrov E. M. , Zvonkov D. A. , Khankhasova T. D. , Voropaeva A. V. , Krutalevich Yu. M. , Cheremnov V. S. , Obarchuk O. G.
Published: April 2022

Introduction. One of the actual problems of reconstructive colorectal surgery in childhood is a high frequency of complications after the initial reconstructive procedures for Hirschprung disease. Currently, in the clinical practice there is a wide range of surgical techniques, the combination of which allows to avoid serious outcomes leading to child’s disability. The choice of the volume of repeated surgeries is strictly individual, depending on the nature of complications, types of previously performed surgeries as well as on patient’s initial status. The key for developing a curative tactics is findings after instrumental and morphological examination which were obtained for previous interventions and which often require additional further clarification.Material and methods. In 2010–2021, in the Center of Pediatric Colorectal Anomalies (Irkutsk, Russia) redo endorectal pullthrough surgeries were performed in 18 patients with Hirschprung disease after previous correcting interventions. Surgeons made transanal resection in combination with posterior sagittal perineotomy. Indications for repeated surgical procedures, in most cases, were residual aganglionic segments and the constipation relapses – 11 (61,2%) patients; scar deformation and anus channel stenosis – 5 (27,8%) patients; stricture of coloanal anastomosis – 1 (5,5%) patient; scar stenosis of the neorectum with a rectobulbar fistula – 1 (5,5%) patient.Results. 13 (72,2%) patients had good clinical outcomes: no parents’ complaints, stable rhythms of defecation (from 1 to 4 times per day), feces contents (type 3–6 by Bristol scale of feces). In 4 (22,2%) cases, there was chronic constipation where defecation was 2–3 times a week with preserved defecation urgencies and rare episodes of anal overflow incontinence requiring conservative treatment under the bowel emptying program. One patient had a stricture of coloanal anastomosis which was corrected with its dissection and further dilatation.Conclusion. To confirm indications for redo surgeries, a comprehensive assessment of the colon and perineum with clinical, functional and morphological examination is required. Transanal pull-down is a relatively safe procedure which, in most cases, has good and satisfactory clinical postoperative results and which may be performed even after previous endorectal resections. The rehabilitation program after the discussed surgery is strictly personified and developed under close interaction of a surgeon and a gastroenterologist after assessing the state of the formed coloanal anastomosis and the distal part of the pulled-down intestine.
Anesthetic and medical properties of xenon in the preparation of children for plastic wound closure
Authors: Raushenbakh N. G. , Bagaev V. G. , Amcheslavsky V. G. , Ivanova T. F. , Dzhanmirzoeva A. Yu. kyzy
Published: April 2022

Introduction. The authors present their experience in experimental and clinical studies on the application of Xenon (Xe) in subnarcotic concentrations for pain relief and for the treatment of acute stress disorders. Preparing wounds for plastic closure in children is a long and painful process which requires many anesthesia procedures during dressings. It leads to cognitive disorders, affects memory and learning potentials. Every third child who survived a severe trauma suffers of acute stress disorders: bad sleep, emotional imbalance, anxiety, asociality, inappropriate actions. Xenon is a non-toxic anesthetic. In the concentration of 20–50%, it is not only anesthetizes, but also decreases acute stress disorders, has sedative and anti-stress effects and normalizes sleep.Objectives. To study literature data on the potentials of gas Xe in subnarcotic concentrations for pain relief and for decreasing acute stress disorders at the stage of preparing a child for plastic closure of a wound defect.Material and methods. Literature search was done in PubMed, Scopus, eLIBRARY, Cyberleninka in English and Russian using key words: “Xenon”, “Xenon and anesthesia”, “Xenontherapy” “Actions of Xenon”, “Treatment of extensive wounds in children”, “Acute stress disorders”.Results. The analysis of the data of the conducted studies showed the non-toxicity of Xe, which makes it possible to use it repeatedly for anesthesia in children. The fact that Xe reduces the manifestations of anxiety, depressive and psychosomatic symptoms in patients with acute and chronic stress disorders allows us to count on its positive effects in the treatment of children with extensive wounds of traumatic origin at the stage of preparing them for plastic closure.Conclusion. The practical absence in the available scientific literature of a study to assess the effectiveness of the use of subnarcotic concentrations of Xe, when dressing extensive wounds at the stage of preparation of the wound process for plastic closure in children with acute stress disorders, justifies the conduct of an appropriate scientific study.
Systemic arterial blood supply in the spectrum of bronchopulmonary vascular malformations
Authors: Yudin A. L. , Razumovskiy A. Yu. , Afukova O. A. , Abovich Yu. A. , Yumatova E. A.
Published: April 2022

Introduction. Congenital bronchopulmonary vascular malformations manifest a wide range of disorders in the three main components of the lungs: respiratory tract and lung parenchyma, arteries and veins. Malformations of all the main components of lung tissues, namely, arterial blood supply, venous outflow and respiratory tract - may be variants of a single primary complex of pulmonaryvascular anomalies.Observation. A described clinical case demonstrates an extremely interesting mixed bronchoarteriovenous malformation affecting the pulmonary respiratory tract, pulmonary artery and pulmonary vein. Various instrumental techniques were used for the diagnostics, the key of which was computed tomography with intravenous contrast.Conclusion. The article presents a wide range of pathological changes observed in this clinical case; it also analyzes the nature of each component and terminology.
Kétoldali panuveitis esete 59 éves, immunszupprimált nőbetegnél
Authors: Gyenes Andrea , Mihályi Dorottya , Várady Erika , Papp András , Nagy Zoltán Zsolt , Géhl Zsuzsanna
Published: April 2022

Az alábbiakban egy 59 éves nőbeteg esetét mutatjuk be, aki jobb szemének látásromlását követően 6 héttel jelentkezett a területi szakrendelőben, ahonnan klinikánkra utalták kétoldali panuveitis diagnózissal. Mindkét oldalon észlelt masszív üvegtesti homályok mellett a jobb szemen a retina nem vizsgálható, bal oldalon kiterjedt fehér infiltrátum és fundusszerte tócsás vérzések látszottak. A hipertóniával és 2-es típusú diabetes mellitussal kezelt nőbeteg anamnéziséből a látásromlást megelőzően másfél évvel diagnosztizált, majd kemoterápiával és biológiai terápiával kezelt mérsékelt malignitású B-sejtes non-Hodgkin-lymphoma emelendő ki. Kivizsgálása kezdődött, amely a látásromlás hátterében cytomegalovírus (CMV) retinitis fennállását igazolta. Ezzel egy időben vastagbél térfoglaló folyamatának eltávolítása történt, a tumor kórszövettani vizsgálata polipoid CMV-asszociált vaszkuláris proliferációt mutatott.