bluuebunny/crossref_metadata_2025 huggingface dataset:
Effects of three different protein levels on the growth, gonad development, and physiological biochemistry of female Pengze crucian carp (Carassius auratus var. Pengze) broodstock
Authors: Xiao Jun , Long Fan , Ding Liyun , Yao Yuan , Wu Weihua , Fu Yilong , Chen Wenjing
Published: August 2024

A 56-days feeding experiment was conducted to evaluate the effect of dietary protein levels on fish growth, gonad development, and physiological biochemistry of female Pengze crucian carp (Carassius auratus var. Pengze) broodstock. Three isoenergetic diets were formulated, and the crude protein levels were 26.85%, 35.73% and 44.38%, the lipid levels were 7.39%, 7.92% and 7.91%, respectively. The results showed that the weight gain rate (WGR), the specific growth rate (SGR) and the protein efficiency ratio (PER) of the female Pengze crucian carp broodstock increased significantly and the feed conversion ratio (FCR) decreased significantly when the dietary protein level was 35.73% (P < 0.05). The WGR, the SGR and the PER were significantly higher in the 35.73% and 44.38% protein groups than in the 26.85% protein group (P<0.05). Increasing dietary protein levels led to a significant increase in the gonad index and the egg menstrual length of the female Pengze crucian carp broodstock (P<0.05). The higher protein levels resulted in a significant increase in total amino acids, total essential amino acids, and total nonessential amino acids (P<0.05). The varying ovaries of each group of total saturated fatty acids, total highly unsaturated fatty acids, and total polyunsaturated fatty acids all increased significantly with increasing protein levels (P<0.05). Furthermore, the aspartate transaminase (AST), low-density lipoprotein cholesterol (LDLC), and malondialdehyde (MDA) contents in plasma were significantly lower in the 35.73% and 44.38% groups than in the 26.85% group (P < 0.05). The 35.73% group exhibited the highest plasma superoxide dismutase (SOD) activity,. The plasma vitellogenin (VTG), estradiol 2 (E2), and progesterone (PROG) in plasma of female Pengze crucian carp broodstock were significantly higher in the 35.73% and 44.38% protein groups than in the 26.85% protein group (P<0.05). The results showed that the addition of an appropriate amount of protein to the feed can significantly improve the growth, gonadal development, and health of female Pengze crucian carp. In this study, the effect of 35.73% protein concentration was better than in the other two groups.
Revealing the regulation of allergic asthma airway epithelial cell inflammation by STEAP4 targeting MIF through machine learning algorithms and single-cell sequencing analysis
Authors: Qiao Lu , Li Shi-meng , Liu Jun-nian , Duan Hong-lei , Jiang Xiao-feng
Published: August 2024

Asthma comprises one of the most common chronic inflammatory conditions, yet still lacks effective diagnostic markers and treatment targets. To gain deeper insights, we comprehensively analyzed microarray datasets of airway epithelial samples from asthmatic patients and healthy subjects in the Gene Expression Omnibus database using three machine learning algorithms. Our investigation identified a pivotal gene, STEAP4. The expression of STEAP4 in patients with allergic asthma was found to be reduced. Furthermore, it was found to negatively correlate with the severity of the disease and was subsequently validated in asthmatic mice in this study. A ROC analysis of STEAP4 showed the AUC value was greater than 0.75. Functional enrichment analysis of STEAP4 indicated a strong correlation with IL-17, steroid hormone biosynthesis, and ferroptosis signaling pathways. Subsequently, intercellular communication analysis was performed using single-cell RNA sequencing data obtained from airway epithelial cells. The results revealed that samples exhibiting low levels of STEAP4 expression had a richer MIF signaling pathway in comparison to samples with high STEAP4 expression. Through both in vitro and in vivo experiments, we further confirmed the overexpression of STEAP4 in airway epithelial cells resulted in decreased expression of MIF, which in turn caused a decrease in the levels of the cytokines IL-33, IL-25, and IL-4; In contrast, when the STEAP4 was suppressed in airway epithelial cells, there was an upregulation of MIF expression, resulting in elevated levels of the cytokines IL-33, IL-25, and IL-4. These findings suggest that STEAP4 in the airway epithelium reduces allergic asthma Th2-type inflammatory reactions by inhibiting the MIF signaling pathway.
Gut microbiota changes in patients with Alzheimer’s disease spectrum based on 16S rRNA sequencing: a systematic review and meta-analysis
Authors: Li Hui , Cui Xiaopan , Lin Yuxiu , Huang Fengqiong , Tian Ayong , Zhang Rongwei
Published: August 2024

Background

The gut microbiota (GM) is hypothesized to play roles in Alzheimer’s disease (AD) pathogenesis. In recent years, many GM composition and abundance investigations in AD patients have been conducted; however, despite this work, some results remain controversial. Therefore, we conducted a systematic review and meta-analysis using 16S ribosomal RNA (16S rRNA) sequencing to explore GM alterations between patients with AD spectrum and healthy controls (HCs).

Methods

A systematic and comprehensive literature search of PubMed, Web of Science, Embase, the Cochrane Library, China National Knowledge Infrastructure, China Biology Medicine disc database, WanFang database and Social Sciences Citation Index databases was conducted from inception to January 2023. Inclusion and exclusion criteria were strictly defined, and two researchers independently screened and extracted information from selected studies. Data quality were evaluated according to the “Cochrane system evaluator manual” and pooled data were comprehensively analyzed using Stata 14 software with standardized mean differences (SMDs) and 95% confidence intervals (95% CIs) used to measure effect sizes. Also, geographical heterogeneity effects (related to cohorts) on GM abundance were examined based on subgroup meta-analyses if sufficient studies reported outcomes. Finally, publication bias was assessed using funnel plots.

Results

Out of 1566 articles, 13 studies involving 581 patients with AD spectrum and 445 HCs were deemed eligible and included in our analysis. In summary, a decreased microbiota alpha diversity and a significantly distinct pattern of clustering with regard to beta diversity were observed in AD spectrum patients when compared with HCs. Comparative analyses revealed a decreased Ruminococcus, Faecalibacterium, Lachnospira, Dialister, Lachnoclostridium, and Roseburia abundance in AD spectrum patients while Phascolarctobacterium, Lactobacillus, and Akkermansia muciniphila were more enriched in patients when compared to HCs. Furthermore, regional variations may have been in play for intestinal microbes such as Bacteroides, Bifidobacterium, and Alistipes.

Conclusion

Our meta-analysis identified alterations in GM abundance in patients with AD spectrum, with 12 genera from four major phyla significantly associated with AD. Moreover, we provided evidence for region-specific alterations in Bacteroides, Bifidobacterium, and Alistipes abundance. These findings may have profound implications for the development of innovative GM-based strategies to prevent and treat AD.

Systematic review registration

https://doi.org/10.37766/inplasy2024.6.0067, identifier INPLASY202460067.
Bidirectional negative relationship between thyrotropin and kidney function during alcohol intoxication in males
Authors: Unlu Hayrunnisa , Yehia Asmaa , Manji Khalid , Manji Noah , Treviño-Alvarez Andrés M. , Cabeza De Baca Tommy , Frye Mark A. , Thomas Leslie F. , Abulseoud Osama A.
Published: August 2024

Introduction

Despite a well-established direct toxic effect of alcohol on renal cells, there is a salutary dose-dependent effect of alcohol consumption on common laboratory parameters related to kidney performance. Alcohol also impacts thyroid hormones, while thyroid status modulates kidney function. The modulation of kidney parameters with thyrotropin (TSH) and thyroid status indicates a possible interaction between alcohol, kidney, and thyroid functions. This retrospective study was conducted to test the hypothesis that the positive effect of alcohol use on the estimated glomerular filtration rate (eGFR) is mediated by alcohol’s effect on thyroid hormones.

Methods

We reviewed the electronic medical records of 767 hospitalized adult patients free of thyroid disorders who received medical care in the Mayo Clinic Health System from June 2019 through June 2022 and had blood alcohol concentration (BAC), serum TSH, and serum creatinine measured during the hospitalization. We calculated the eGFR using both the re-expressed Modification of Diet in Renal Disease (MDRD II) study equation and the Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) Creatinine equation.

Results

We found a significant relationship of BAC with eGFR (CKD-EPI) and TSH in males only. BAC had a positive association with eGFR (b = 0.24, p = 0.0001) and negative with TSH (b=-0.17, p = 0.006). The covariance between the two outcomes (eGFR and TSH) was negative (b = -0.12, p = 0.049). The path analyses using the eGFR MDRD II equation were not significant in males, whereas females had no significant path analyses with either of the eGFR equations.

Discussion

We observed that BAC influences both eGFR and TSH, whereas eGFR and TSH influence each other. After considering important covariates (e.g., age, body mass index, diabetes mellitus, cardiovascular disease, chronic kidney disease, and chronic liver disease) and the negative bidirectional effect of TSH and eGFR, a positive impact of BAC on eGFR was observed in males.
Case report: Bridging limbic network epilepsy with psychiatric, memory, and sleep comorbidities: case illustrations of reversible psychosis symptoms during continuous, high-frequency ANT-DBS
Authors: Wheeler Lydia , Worrell Samuel E. , Balzekas Irena , Bilderbeek Jordan , Hermes Dora , Croarkin Paul , Messina Steven , Van Gompel Jamie , Miller Kai J. , Kremen Vaclav , Worrell Gregory A.
Published: August 2024

The network nature of focal epilepsy is exemplified by mesial temporal lobe epilepsy (mTLE), characterized by focal seizures originating from the mesial temporal neocortex, amygdala, and hippocampus. The mTLE network hypothesis is evident in seizure semiology and interictal comorbidities, both reflecting limbic network dysfunction. The network generating seizures also supports essential physiological functions, including memory, emotion, mood, and sleep. Pathology in the mTLE network often manifests as interictal behavioral disturbances and seizures. The limbic circuit is a vital network, and here we review one of the most common focal epilepsies and its comorbidities. We describe two people with drug resistant mTLE implanted with an investigational device enabling continuous hippocampal local field potential sensing and anterior nucleus of thalamus deep brain stimulation (ANT-DBS) who experienced reversible psychosis during continuous high-frequency stimulation. The mechanism(s) of psychosis remain poorly understood and here we speculate that the anti-epileptic effect of high frequency ANT-DBS may provide insights into the physiology of primary disorders associated with psychosis.
Effectiveness of active middle ear implant placement methods in pathological conditions: basilar membrane vibration simulation
Authors: Lee Sinyoung , Motegi Masaomi , Koike Takuji
Published: August 2024

Active middle ear implants (AMEI) amplify mechanical vibrations in the middle ear and transmit them to the cochlea. The AMEI includes a floating mass transducer (FMT) that can be placed using two different surgical approaches: “oval window (OW) vibroplasty” and “round window (RW) vibroplasty.” The OW and RW are windows located on the cochlea. Normally, sound stimulus is transmitted from the middle ear to cochlea via the OW. RW vibroplasty has been suggested as an alternative method due to the difficulty of applying OW vibroplasty in patients with ossicle dysfunction. Several reports compare the advantages of each approach through pre and postoperative hearing tests. However, quantitatively assessing the treatment effect is challenging due to individual differences in pathologies. This study investigates the vibration transmission efficiency of each surgical approach using a finite-element model of the human cochlea. Vibration of the basilar membrane (BM) of the cochlea is simulated by applying the stimulus through the OW or RW. Pathological conditions, such as impaired stapes mobility, are simulated by increasing the stiffness of the stapedial annular ligament. RW closure due to chronic middle ear diseases is a common clinical occurrence and is simulated by increasing the stiffness of the RW membrane in the model. The results show that the vibration amplitude of the BM is larger when the stimulus is applied to the RW compared to the OW, except for cases of RW membrane ossification. The difference in these amplitudes is particularly significant when stapedial mobility is limited. These results suggest that RW vibroplasty would be advantageous, especially in cases of accompanying stapedial mobility impairment. Additionally, it is suggested that transitioning to OW vibroplasty could still ensure a sufficient level of vibratory transmission efficiency when placing the FMT on the RW membrane is difficult due to anatomical problems in the tympanic cavity or confirmed severe pathological conditions around the RW.
Network-based transcranial direct current stimulation enhances attention function in healthy young adults: a preliminary study
Authors: Wei Xiaoyu , Zhou Rong , Zheng Suwang , Zhang Yufeng , Feng Xiaofan , Lü Jiaojiao
Published: August 2024

Purpose

Attention, a complex cognitive process, is linked to the functional activities of the brain’s dorsal attention network (DAN) and default network (DN). This study aimed to investigate the feasibility, safety, and blinding efficacy of a transcranial direct current stimulation (tDCS) paradigm designed to increase the excitability of the DAN while inhibiting the DN (DAN+/DN-tDCS) on attention function in healthy young adults.

Methods

In this randomized controlled experiment, participants were assigned to either the DAN+/DN-tDCS group or the sham group. A single intervention session was conducted at a total intensity of 4 mA for 20 min. Participants completed the Attention Network Test (ANT) immediately before and after stimulation. Blinding efficacy and adverse effects were assessed post-stimulation.

Results

Forty participants completed the study, with 20 in each group. Paired-sample t-test showed a significant post-stimulation improvement in executive effect performance (t = 2.245; p = 0.037) in the DAN+/DN-tDCS group. The sham group did not exhibit any significant differences in ANT performance. Participants identified the stimulation type with 52.50% accuracy, indicating no difference in blinding efficacy between groups (p = 0.241). Mild-to-moderate adverse effects, such as stinging, itching, and skin reddening, were reported in the DAN+/DN-tDCS group (p < 0.05).

Conclusion

DAN+/DN-tDCS enhanced attention function in healthy young individuals, particularly in improving executive effect performance. This study presents novel strategies for enhancing attentional performance and encourages further investigation into the mechanisms and outcomes of these interventions across diverse populations.
Pulmonary neuroendocrine cells: crucial players in respiratory function and airway-nerve communication
Authors: Thakur Abhimanyu , Mei Shuya , Zhang Noel , Zhang Kui , Taslakjian Boghos , Lian Jiacee , Wu Shuang , Chen Bohao , Solway Julian , Chen Huanhuan Joyce
Published: August 2024

Pulmonary neuroendocrine cells (PNECs) are unique airway epithelial cells that blend neuronal and endocrine functions, acting as key sensors in the lung. They respond to environmental stimuli like allergens by releasing neuropeptides and neurotransmitters. PNECs stand out as the only lung epithelial cells innervated by neurons, suggesting a significant role in airway-nerve communication via direct neural pathways and hormone release. Pathological conditions such as asthma are linked to increased PNECs counts and elevated calcitonin gene-related peptide (CGRP) production, which may affect neuroprotection and brain function. CGRP is also associated with neurodegenerative diseases, including Parkinson’s and Alzheimer’s, potentially due to its influence on inflammation and cholinergic activity. Despite their low numbers, PNECs are crucial for a wide range of functions, highlighting the importance of further research. Advances in technology for producing and culturing human PNECs enable the exploration of new mechanisms and cell-specific responses to targeted therapies for PNEC-focused treatments.
Development and validation of a prognostic nomogram to predict the recurrence of AFP-negative and DCP-positive hepatocellular carcinoma after curative resection
Authors: Li Junnan , Wang Qi , Yan Yadong , Sun Lina , Zhang Gongming , Li Guangming , Jin Ronghua
Published: August 2024

Purpose

Approximately one-third of hepatocellular carcinoma (HCC) cases are characterized by alpha-fetoprotein (AFP) negativity (AFP-NHCC. Among these patients, around 60% exhibit des-gamma-carboxyprothrombin (DCP) positivity, and DCP-positive patients have a poorer prognosis. As a curative treatment, recurrence after liver resection poses significant challenges to the prognosis of HCC patients. Therefore, it is necessary to determine the relevant risk factors of these patients and provide timely treatment options.

Methods

This study included 540 patients who underwent resection at Beijing You’an Hospital. 292 patients from 2014 to 2018 constituted the training cohort, while 248 patients from 2018 to 2020 constituted the validation cohort. All patients underwent routine follow-ups until December 2023. Variables were identified through Cox regression, and a nomogram was developed. The nomogram was evaluated using time-dependent receiver operating characteristic curves (ROC), calibration curves, Decision curve analysis (DCA), and Kaplan-Meier (KM) curve analysis

Results

We found that age, tumor number, tumor size, γ-glutamyl transpeptidase (γ-GT), and prothrombin time (PT) are independent risk factors for HCC recurrence, and a nomogram was developed and validated based on this result to predict recurrence-free survival (RFS) at 1, 2, and 3 years. The performance of the nomogram was further confirmed by the ROC curve, calibration curve, and DCA, all of which showed favorable results. The KM curve analysis clearly distinguishes between two groups of people with different risks in terms of prognosis in both the training and validation sets.

Conclusion

In summary, we established and validated a novel nomogram by multivariate Cox regression analysis to predict recurrence in DCP-positive patients with AFP-NHCC after resection. The nomogram, including age, tumor number, tumor size, γ-GT, and PT, demonstrates better predictive ability for AFP-NHCC patients with DCP positive.
Therapeutic targeting of PARP with immunotherapy in acute myeloid leukemia
Authors: Bian Xing , Liu Wenli , Yang Kaijin , Sun Chuanbo
Published: August 2024

Targeting the poly (ADP-ribose) polymerase (PARP) protein has shown therapeutic efficacy in cancers with homologous recombination (HR) deficiency due to BRCA mutations. Only small fraction of acute myeloid leukemia (AML) cells carry BRCA mutations, hence the antitumor efficacy of PARP inhibitors (PARPi) against this malignancy is predicted to be limited; however, recent preclinical studies have demonstrated that PARPi monotherapy has modest efficacy in AML, while in combination with cytotoxic chemotherapy it has remarkable synergistic antitumor effects. Immunotherapy has revolutionized therapeutics in cancer treatment, and PARPi creates an ideal microenvironment for combination therapy with immunomodulatory agents by promoting tumor mutation burden. In this review, we summarize the role of PARP proteins in DNA damage response (DDR) pathways, and discuss recent preclinical studies using synthetic lethal modalities to treat AML. We also review the immunomodulatory effects of PARPi in AML preclinical models and propose future directions for therapy in AML, including combined targeting of the DDR and tumor immune microenvironment; such combination regimens will likely benefit patients with AML undergoing PARPi-mediated cancer therapy.
Long-term efficacy and reduced side-effects of buprenorphine in patients with moderate and severe chronic pain
Authors: Papa Alfonso , Salzano Anna Maria , Di Dato Maria Teresa , Desiderio Vincenzo , Buonavolontà Pietro , Mango Pietro , Saracco Elisabetta , Tammaro Dario , Luongo Livio , Maione Sabatino
Published: August 2024

Background

Chronic pain significantly impacts quality of life and poses substantial public health challenges. Buprenorphine, a synthetic analog of thebaine, is recognized for its potential in managing moderate to severe chronic pain with fewer side effects and a lower incidence of tolerance compared to traditional opioids.

Objective

This retrospective study aimed to assess the long-term efficacy and safety of buprenorphine transdermal patches in patients with moderate and severe chronic pain, with a focus on pain relief sustainability and tolerance development.

Methods

This retrospective observational study involved 246 patients prescribed buprenorphine transdermal patches. We evaluated changes in pain intensity using the Numeric Rating Scale (NRS), assessed opioid tolerance based on FDA guidelines for morphine-equivalent doses, and measured patient-reported outcomes through the Patients’ Global Impression of Change (PGIC). Any adverse events were also recorded.

Results

Over the 36-month period, there was a significant reduction in NRS scores for both moderate and severe pain patients, demonstrating buprenorphine’s sustained analgesic effect. Tolerance measurement indicated that no patients required increases in morphine-equivalent doses that would meet or exceed the FDA’s threshold for opioid tolerance (60 mg/day of morphine or equivalent). Additionally, patient satisfaction was high, with the PGIC reflecting significant improvements in pain management and overall wellbeing. The side effects were minimal, with skin reactions and nausea being the most commonly reported but manageable adverse events.

Conclusion

The study findings validate the long-term use of buprenorphine transdermal patches as an effective and safe option for chronic pain management, maintaining efficacy without significant tolerance development. These results support the continued and expanded use of buprenorphine in clinical settings, emphasizing its role in reducing the burdens of chronic pain and opioid-related side effects. Further research is encouraged to refine pain management protocols and explore buprenorphine’s full potential in diverse patient populations.
Multifactorial analysis and experiments affecting the effect of fog droplet penetration in fruit tree canopies
Authors: Sun Daozong , Huang Xinghan , Hu Junyutai , Jiang Haoliang , Song Shuran , Xue Xiuyun
Published: August 2024

This study examines the impact of canopy density, side wind speed, nozzle tilt angle, and droplet size on droplet penetration during plant protection spraying operations. Experiments conducted in citrus orchards evaluated how side wind speed and nozzle tilt angle influence droplet penetration across various canopy densities. A Phase Doppler Analyzer (PDA) was used to assess droplet size variations under different nozzle tilt angles and side wind speeds, yielding a multiple linear regression equation (R2 = 0.866) that links nozzle tilt angle and side wind speed with droplet size. Results showed that droplet size decreases with increasing nozzle tilt angle at a constant crosswind speed. Further experiments investigated the effects of droplet size and canopy leaf area density on droplet penetration, involving three canopy leaf area densities, four wind speeds, and six nozzle tilt angles. Droplet deposition and canopy coverage were measured under various spraying parameters, with conventional operations (0° nozzle tilt and orthogonal wind speeds) serving as controls. The study found that adjusting nozzle tilt angle and wind speed enhances droplet penetration in different canopy structures. Optimal parameters varied with leaf area density (LAD): an 18° tilt angle and 3 m/s wind speed for a LAD of 5.94 m3/m3, a 45° tilt angle and 2 m/s wind speed for a LAD of 8.47 m2/m3, and a 36° tilt angle and 3 m/s wind speed for a LAD of 11.12 m2/m3. At 1 m/s, droplet deposition followed a downward parabolic trend with changes in nozzle tilt angle, whereas at 2 m/s, deposition followed an upward parabolic trend. At a side wind speed of 3 m/s, droplet deposition remained unchanged with nozzle tilt angle but decreased with increasing canopy density. Nonlinear regression analysis indicated that leaf area density had a greater impact on deposition differences than droplet size, with droplet penetration decreasing as leaf area density increased. This study provides a reference for enhancing fog droplet penetration techniques in plant protection operations, offering practical guidelines for optimizing spraying conditions and improving pesticide use efficiency in different canopy structures.
Straw return enhances grain yield and quality of three main crops: evidence from a meta-analysis
Authors: Zhang Ruipeng , Yu Haiyang , Zhang Wenbiao , Li Wei , Su Hao , Wu Sixuan , Xu Qiong , Li Yaying , Yao Huaiying
Published: August 2024

Straw return is regarded as a widely used field management strategy for improving soil health, but its comprehensive effect on crop grain yield and quality remains elusive. Herein, a meta-analysis containing 1822 pairs of observations from 78 studies was conducted to quantify the effect of straw return on grain yield and quality of three main crops (maize, rice, and wheat). On average, compared with no straw return, straw return significantly (p< 0.05) increased grain yield (+4.3%), protein content (+2.5%), total amino acids concentration (+1.2%), and grain phosphorus content (+3.6%), respectively. Meanwhile, straw return significantly (p< 0.05) decreased rice chalky grain rate (-14.4%), overall grain hardness (-1.9%), and water absorption of maize and wheat (-0.5%), respectively. Moreover, straw return effects on grain yield and quality traits were infected by cultivated crop types, straw return amounts, straw return methods, and straw return duration. Our findings illustrated that direct straw return increased three main crop grain yields and improved various quality traits among different agricultural production areas. Although improper straw return may increase plant disease risk and affect seed germination, our results suggest that full straw return with covered or plough mode is a more suitable way to enhance grain yield and quality. Our study also highlights that compared with direct straw return, straw burning or composting before application may also be beneficial to farmland productivity and sustainability, but comparative studies in this area are still lacking.
Characterization of miRNA profiling in konjac-derived exosome-like nanoparticles and elucidation of their multifaceted roles in human health
Authors: Shen Chuan , Li Xia , Qin Jianfeng , Duan Longfei
Published: August 2024

Plant-derived exosome-like nanoparticles (ELNs) have demonstrated cross-kingdom capabilities in regulating intercellular communication, facilitating drug delivery, and providing therapeutic interventions in humans. However, the functional attributes of konjac-derived ELNs (K-ELNs) remain largely unexplored. This study investigates the isolation, characterization, and functional analysis of K-ELNs, along with the profiling and differential expression analysis of associated miRNAs in both K-ELNs and Konjac tissues. K-ELNs were successfully isolated and characterized from two konjac species using ultracentrifugation, followed by Transmission Electron Microscopy (TEM) and Nanoparticle Tracking Analysis (NTA). Small RNA sequencing identified a total of 3,259 miRNAs across all samples. Differential expression analysis revealed significant differences in miRNA profiles between K-ELNs and tissue samples. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) functional enrichment analysis of target genes provided insights into their roles in modulating pathways associated with diseases such as cancer and neurodegenerative disorders. Additionally, six miRNAs were selected for validation of sequencing results via RT-qPCR. The 5’RLM-RACE method was employed to validate the cleavage sites between differentially expressed miRNAs (DEMs) and their predicted target genes, further substantiating the regulatory roles of miRNAs in konjac. The findings of this study enhance our understanding of the molecular mechanisms underlying the biological functions and applications of K-ELNs, laying the groundwork for future research into their potential therapeutic roles in human health.
Assessment of children suicide attempts frequency in the peripandemic period
Authors: Wiktor Łukasz , Damps Maria
Published: August 2024

Objectives

Our study aimed to evaluate patients after suicide attempts treated at the Department of Trauma Surgery for Children in the peripandemic period, assessment of potential risk factors, and the school’s participation as the unit responsible for the prevention of suicidal behavior.

Materials and methods

Retrospective review of the medical database at equal time intervals of 24 months to identify patients treated before and after the COVID-19 was done. Thorough analysis including injury mechanism, medical procedures, history of previous mental disorders or suicidal behavior was performed. Furthermore, results were compared with the Polish police suicide statistics.

Results

Based on our retrospective review we found 4 patients treated in our department before the pandemic and 10 patients treated after COVID-19 outbreak. The group before SARS-Cov-2 era consisted of three girls and one boy with a mean age of 14.97 (12.7–17.6). The group treated in the pandemic crisis consisted of 8 boys and 2 girls, the mean age was 15.49 (10.8–17.2). In the pre-COVID-19 group, 2 out of 4 patients had received psychiatric treatment before, but none had attempted suicide before. In the COVID-19 group, 6 out of 10 patients had previously received psychiatric treatment, moreover 3 of them attempted suicide before. Based on our analysis, the number of individuals who displayed suicidal attempts has raised. Between 2018 and 2021 the largest number of suicides concerned the 13–18 y.o. group, both for the Silesian Voivodeship (H = 9.374; p = 0.0092) and for the whole country (H = 10.203; p = 0.0061).

Conclusion

(1) Results of our study indicate that the pandemic may have caused a wide range of negative mental health consequences for young individuals; (2) Suicide attempts in children are often related with high energy trauma; (3) Teachers and school psychologists, as well as medical health providers, should be aware of rising suicide rates among adolescents.
Well-being balance and lived experiences assessment: a valid, comprehensive measure of positive well-being
Authors: McDermott Ashley F. , Brydges Christopher R. , Norris Troy W.
Published: August 2024

Introduction

Widely used measures of self-reported subjective well-being and flourishing generally do not directly measure positive experiences that have been demonstrated to improve subjective well-being and flourishing, which could aid in developing personalized interventions to improve individuals’ well-being. The present study evaluated the validity of the Well-being Balance and Lived Experience (WBAL) Model and Assessment, a new model of well-being and corresponding assessment instrument that evaluates the self-reported frequency of positive experiences and positive feelings of well-being, balanced across activation and arousal levels.

Methods

A total of 496 evaluable subjects completed the WBAL Assessment, the PERMA+ Profiler (PERMA+) and the Well-Being Assessment Adult 24-item (WBA-24). A confirmatory factor model corresponding to the WBAL construct was created, and internal and external validity of the WBAL Assessment were interrogated.

Results

The confirmatory factor model showed good fit, indicating that each of the model factors are related but distinct and all items load significantly onto their factors. The WBAL Assessment demonstrated high internal consistency (Cronbach’s α = 0.95) and internal validity across well-being factors and Feelings (r = 0.96) and Experiences (r = 0.94) domains. The WBAL Assessment demonstrated strong convergent validity in comparison to PERMA+ (r = 0.80) and WBA-24 (r = 0.75), indicating that the WBAL Assessment measures a similar overall concept of well-being and flourishing. Discriminant validity of WBAL factors was demonstrated for an average of 14.3 of 17 comparator domains. The main differences between instruments are WBAL’s assessment of positive Experiences, the comparator instruments’ inclusion of feelings with negative valence, and WBA-24’s inclusion of financial stability.

Discussion

The WBAL Assessment is a reliable and valid instrument to comprehensively measure positive aspects of well-being that evaluates multiple modifiable contributors to individuals’ well-being to guide design of personalized assessment and intervention programs to enhance positive well-being.
What do families value most about the care home where their older adult relatives live?
Authors: Leché-Martín Eduardo Alberto , Saz-Gil María Isabel , Gil-Lacruz Ana Isabel , Sierra-Berdejo María José , Gil-Lacruz Marta
Published: August 2024

Introduction

The 2030 Agenda and the principles of Corporate Social Responsibility (CSR) define companies and public authorities as agents for social change sharing objectives such as promotion of health, personal development and social engagement, among others. Care homes for the older adult are an example of organizations that should be particularly aware of these priorities. Since they work with vulnerable groups, collaboration with the families is essential in ensuring residents' wellbeing.

Methods

The objective of this study is to analyse the factors that condition the satisfaction of relatives of residents in a care home for the older adult located in a rural environment in the province of Huesca (Spain).

Results and discussion

The 51 relatives interviewed rated the following points very positively: location and accessibility, food service, medical resources, communication with the staff and management team. A high percentage, however, did not know about the channels for volunteer work and institutional involvement. Some psychosocial indicators related to families' interaction and communication with the staff and their potential involvement in the dynamics of the institution have considerable weight in how they explain their satisfaction. These results may lead to new lines of research and intervention that contribute to improving the quality of this type of resources and their commitment to the Sustainable Development Goals (SDGs) and social responsibility.
Disparities and protective factors in pandemic-related mental health outcomes: a Louisiana-based study
Authors: Rung Ariane L. , Oral Evrim , Prusisz Tyler , Peters Edward S.
Published: August 2024

Introduction

The COVID-19 pandemic has had a wide-ranging impact on mental health. Diverse populations experienced the pandemic differently, highlighting pre-existing inequalities and creating new challenges in recovery. Understanding the effects across diverse populations and identifying protective factors is crucial for guiding future pandemic preparedness. The objectives of this study were to (1) describe the specific COVID-19-related impacts associated with general well-being, (2) identify protective factors associated with better mental health outcomes, and (3) assess racial disparities in pandemic impact and protective factors.

Methods

A cross-sectional survey of Louisiana residents was conducted in summer 2020, yielding a sample of 986 Black and White adults. The exposure was overall pandemic impact, measured using the Epidemic-Pandemic Impacts Inventory, and the outcome was general well-being (GWB), measured with the General Well-Being Schedule. Potential protective factors included social support, resilience, and social cohesion. Linear regression models were constructed to examine the association between pandemic impact and GWB, with each protective factor added as an effect modifier. These relationships were further assessed for differences by race.

Results

Pandemic stressors can be grouped into social, health, work, finance, and family-related impacts. Black persons displayed higher levels of pandemic impact as well as lower levels of social support, resilience, and social cohesion (p < 0.0001), highlighting existing racial disparities, though Black respondents and White respondents exhibited no differences in general-well being. Social support, resilience, and social cohesion were identified as protective factors for both groups (p < 0.0001, respectively), but these protective effects deteriorated as pandemic impacts increased. The addition of a pandemic impact by race interaction term was also significant in each model (p = 0.0020, p < 0.0001, and p = 0.0095, respectively) and showed that the protective effects of social support and resilience deteriorated more rapidly for Black persons than White persons, while the protective effects of social cohesion deteriorated more rapidly for White persons than Black persons.

Discussion

This study emphasizes the importance of psychosocial resources in buffering the mental health impact of pandemics. It also suggests greater vulnerability for marginalized communities lacking access to crucial support systems. Findings underscore the need for targeted interventions that bolster access to social support, promote resilience, and strengthen social cohesion, particularly within minority groups. Additionally, policymakers should consider proactive measures to assist in recovery and mitigate the disproportionate impact of future crises on vulnerable populations.
Challenges for early diagnosis of neonatal herpes infection in Japan
Authors: Kojima Junya , Suzuki Shunji , Hoshi Shin-Ichi , Sekizawa Akihiko , Sagara Yoko , Matsuda Hideo , Ishiwata Isamu , Kitamura Tadaichi
Published: August 2024

Background

This study aimed to analyze the recent prevalence of neonatal herpes simplex virus infection, maternal symptoms in the presence of neonate who has herpes simplex virus infection, and mode of delivery in Japan.

Methods

We requested 2.078 obstetrical facilities that are members of the Japan Association of Obstetricians and Gynecologists (JAOG) to provide information on neonatal herpes simplex virus infection involving deliveries at or after 22 weeks of gestation between 2020 and 2022. Of these, 1.371 (66.0%) facilities responded with information that could undergo statistical analysis.

Results

There were 10 cases of neonatal herpes simplex virus infection, and the incidence of neonatal herpes simplex virus infection in Japan was about 1 in 1.4 × 105 live births. There were no characteristic maternal findings common to cases of neonatal herpes simplex virus infection.

Conclusion

The incidence of neonatal herpes simplex virus infection in Japan was low. We could not identify any characteristic maternal findings common to cases of neonatal herpes simplex virus infection.
Case report: Successful treatment of hyperkalemia during general anesthesia in a domestic cat
Authors: Irizarry Tiffany , Gradilla Sarah
Published: August 2024

Objective

This study aimed to describe the successful identification and treatment of severe hyperkalemia, cardiac arrhythmia, rhabdomyolysis, and acute kidney injury (AKI) in a domestic cat that underwent general anesthesia for abdominal exploratory surgery. The definitive underlying cause remains unknown; however, a reaction to propofol is suspected.

Case summary

A 6-month-old intact male domestic short-hair cat underwent general anesthesia and developed severe intraoperative rhabdomyolysis, hyperkalemia, ventricular fibrillation, and AKI during surgery despite a documented mild hypokalemia and normal creatinine before inducing anesthesia. Propofol was administered as part of the anesthetic protocol. The patient was resuscitated successfully and responded well to advanced medical intervention. The hyperkalemia and AKI were resolved within less than 24 h from surgery and rhabdomyolysis was resolved at the time of recheck 5 days later.

New or unique information provided

While previously suspected in dogs, to the authors’ knowledge, propofol-related infusion syndrome (PRIS) has not been reported in domestic cats. Veterinary professionals should be aware that drug-induced intraoperative rhabdomyolysis and hyperkalemia can develop unexpectedly and should remain a differential for acute cardiac arrhythmias or cardiac arrest and AKI.
Lactoferrin impairs pathogen virulence through its proteolytic activity
Authors: Ongena Ruben , Dierick Matthias , Vanrompay Daisy , Cox Eric , Devriendt Bert
Published: August 2024

Antibiotics, often hailed as ‘miracle drugs’ in the 20th century, have revolutionised medicine by saving millions of lives in human and veterinary medicine, effectively combatting bacterial infections. However, the escalating global challenge of antimicrobial resistance and the appearance and spread of multidrug-resistant pathogens necessitates research into alternatives. One such alternative could be lactoferrin. Lactoferrin, an iron-binding multifunctional protein, is abundantly present in mammalian secretions and exhibits antimicrobial and immunomodulatory activities. An often overlooked aspect of lactoferrin is its proteolytic activity, which could contribute to its antibacterial activity. The proteolytic activity of lactoferrin has been linked to the degradation of virulence factors from several bacterial pathogens, impeding their colonisation and potentially limiting their pathogenicity. Despite numerous studies, the exact proteolytically active site of lactoferrin, the specific bacterial virulence factors it degrades and the underlying mechanism remain incompletely understood. This review gives an overview of the current knowledge concerning the proteolytic activity of lactoferrins and summarises the bacterial virulence factors degraded by lactoferrins. We further detail how a deeper understanding of the proteolytic activity of lactoferrin might position it as a viable alternative for antibiotics, being crucial to halt the spread of multi-drug resistant bacteria.
The Influence of Climate, Atmospheric Pollution, and Natural Disasters on Cardiovascular Diseases and Diabetes Mellitus in Drylands: A Scoping Review
Authors: Moreira Rafaella Pessoa , da Silva Clara Beatriz Costa , de Sousa Tainara Chagas , Leitão Flávia Lavinnya Betsaida Félix , Morais Huana Carolina Cândido , de Oliveira Andressa Suelly Saturtino , Duarte-Clíments Gonzalo , Gómez María Begoña Sánchez , Cavalcante Tahissa Frota , Costa Alexandre Cunha
Published: August 2024

Objectives

In the face of escalating global aridification, this study examines the complex relationship between climate variability, air pollution, natural disasters, and the prevalence of cardiovascular disease (CVD) and diabetes mellitus (DM) in arid regions.

Methods

The study conducted a scoping review of multiple databases using JBI guidelines and included 74 studies.

Results

The results show that acute myocardial infarction (n = 20) and stroke (n = 13) are the primary CVDs affected by these factors, particularly affecting older adults (n = 34) and persons with hypertension (n = 3). Elevated air temperature and heat waves emerge as critical risk factors for CVD, exacerbating various cardiovascular mechanisms. Atmospheric pollutants and natural disasters increase this risk. Indirect effects of disasters amplify risk factors such as socioeconomic vulnerability (n = 4), inadequate medical care (n = 3), stress (n = 3), and poor diet (n = 2), increasing CVD and DM risk.

Conclusion

The study underscores the need for nations to adhere to the Paris Agreement, advocating for reduced air pollutants, resilient environments, and collaborative, multidisciplinary research to develop targeted health interventions to mitigate the adverse effects of climate, pollution, and natural disasters.
Classification and Regression of Pinhole Corrosions on Pipelines Based on Magnetic Flux Leakage Signals Using Convolutional Neural Networks
Authors: Shen Yufei , Zhou Wenxing
Published: August 2024

Pinhole corrosions on oil and gas pipelines are difficult to detect and size and, therefore, pose a significant challenge to the pipeline integrity management practice. This study develops two convolutional neural network (CNN) models to identify pinholes and predict the sizes and location of the pinhole corrosions according to the magnetic flux leakage signals generated using the magneto-static finite element analysis. Extensive three-dimensional parametric finite element analysis cases are generated to train and validate the two CNN models. Additionally, comprehensive algorithm analysis evaluates the model performance, providing insights into the practical application of CNN models in pipeline integrity management. The proposed classification CNN model is shown to be highly accurate in classifying pinholes and pinhole-in-general corrosion defects. The proposed regression CNN model is shown to be highly accurate in predicting the location of the pinhole and obtain a reasonably high accuracy in estimating the depth and diameter of the pinhole, even in the presence of measurement noises. This study indicates the effectiveness of employing deep learning algorithms to enhance the integrity management practice of corroded pipelines.
Oilseed Rape Yield Prediction from UAVs Using Vegetation Index and Machine Learning: A Case Study in East China
Authors: Hu Hao , Ren Yun , Zhou Hongkui , Lou Weidong , Hao Pengfei , Lin Baogang , Zhang Guangzhi , Gu Qing , Hua Shuijin
Published: August 2024

Yield prediction is an important agriculture management for crop policy making. In recent years, unmanned aerial vehicles (UAVs) and spectral sensor technology have been widely used in crop production. This study aims to evaluate the ability of UAVs equipped with spectral sensors to predict oilseed rape yield. In an experiment, RGB and hyperspectral images were captured using a UAV at the seedling (S1), budding (S2), flowering (S3), and pod (S4) stages in oilseed rape plants. Canopy reflectance and spectral indices of oilseed rape were extracted and calculated from the hyperspectral images. After correlation analysis and principal component analysis (PCA), input spectral indices were screened to build yield prediction models using random forest regression (RF), multiple linear regression (MLR), and support vector machine regression (SVM). The results showed that UAVs equipped with spectral sensors have great potential in predicting crop yield at a large scale. Machine learning approaches such as RF can improve the accuracy of yield models in comparison with traditional methods (e.g., MLR). The RF-based training model had the highest determination coefficient (R2) (0.925) and lowest relative root mean square error (RRMSE) (5.91%). In testing, the MLR-based model had the highest R2 (0.732) and lowest RRMSE (11.26%). Moreover, we found that S2 was the best stage for predicting oilseed rape yield compared with the other growth stages. This study demonstrates a relatively accurate prediction for crop yield and provides valuable insight for field crop management.