bluuebunny/crossref_metadata_2025 huggingface dataset:
SUPLAI DAYA CADANGAN MENGGUNAKAN SISTEM HYBRID
Authors: Awan Setiawan  , Muhammad Fahmi Hakim  , Mudjiono  , Irwan Heyanto/Eryk  , Satrio Pamungkas Bayu Aji Wibowo
Published: November 2022

Pada perusahaan manufaktur peralatan industri, ketika terjadi pemadaman listrik PT PLN, harus menunggu proses transisi suplai daya ke genset sehingga proses produksi harus terhenti sejenak. Dengan adanya hal itu maka perlu didesain suplai daya cadangan baru yang mampu menyuplai daya dengan seketika beserta instalasinya. Suplai daya cadangan yang sesuai adalah Uniterruptible Power Supply (UPS) yang dioperasikan secara hybrid dengan genset. Langkah pertama adalah menentukan kapasitas UPS kemudian menghitung kapasitas baterai dan dilanjutkan menentukan jumlah baterai. Selanjutnya menghitung KHA penghantar beserta arus nominal MCB. Simulasi di ETAP digunakan untuk memeriksa peningkatan performa dari sistem kelistrikan pabrik menggunakan parameter tegangan. UPS yang sesuai berkapasitas 160 kVA, dimensi 560 x 940 x 1800 mm, tegangan keluaran 230/400 V, frekuensi 50-60 Hz dan faktor daya 0,9. Baterai yang digunakan berkapasitas 140 Ah, dimensi 341 x 173 x 281 mm, sejumlah 53 buah. KHA penghantar minimal adalah 80,37 A dan maksimal 161 A. Arus nominal MCB minimal 16 A dan maksimal 126 A. Berdasarkan hasil simulasi sebelum pemasangan UPS, saat suplai utama dari PLN mengalami gangguan, beban prioritas tidak mendapatkan tegangan sampai genset mensuplai energi listrik. Namun setelah pemasangan UPS beban prioritas langsung mendapatkan tegangan dari UPS yang dilanjutkan mendapat suplai dari genset. Beban prioritas yaitu beberapa mesin CNC untuk rposes produksi.
PEMBANGKIT SINUSOIDA PULSE WIDTH MODULATION BERBASIS ARDUINO UNTUK INVERTER
Authors: Mohammad Luqman  , Achmad Komarudin  , Sidik Nurcahyo
Published: November 2022

Kebutuhan energi listrik domestik mayoritas berbentuk gelombang sinusoida pada frekuensi 50 Hz. Sumber energi terbarukan mayoritas berbentuk dc atau daya dengan frekuensi tidak terkontrol. Untuk itu diperlukan alat yang dapat mengontrol bentuk gelombang dan frekuensinya. Alat ini disebut inverter. Inti dari inverter sinusoida terletak pada kontrol switching, dimana bentuk gelombang dan frekuensi ditentukan pada alat kontrol ini, salah satunya adalah gelombang SPWM. Dipasaran tersedia pembangkit SPWM seri EGS-002, modul ini beroperasi pada frekuensi tetap 50 HZ atau 60 Hz, sehingga tidak bisa digunakan untuk membangkitkan daya listrik dengan frekuensi yang variabel. Untuk itu dilakukan penelitian ini, tahap awal adalah membuat pembangkit SPWM berbasiskan arduino dengan spesifikasi semirip mungkin dengan EGS-002. Sistem yang dibangun terdiri dari arduino uno sebagai pembangkit sinyal SPWM, driver IR-2110 sebagai penyedia tegangan mengambang (bootstrap) untuk transistor MOSFET tipe-N IRZF44N pada rangkaian H-bridge. Hasil yang didapatkan adalah mikrokontroller arduino uno bisa membangkitkan sinyal SPWM dengan hasil berupa gelombang sinusoida murni dengan frekuensi 50 Hz.
DC-DC CONVERTER 1300 VA DENGAN PENGENDALI ARUS BERBASIS PENGENDALI ARDUINO MEGA UNTUK APLIKASI CHARGER
Authors: Masramdhani Saputra  , Saddani Djulihenanto  , Imron Ridzki
Published: November 2022

DC-DC converter adalah satu jenis konverter daya yang paling banyak digunakan di berbagai subjek ketenagalistrikan. Hal tersebut dikarenakan semakin banyak aplikasi yang berbasis elektrifikasi teknologi terdahulu seperti mobil listrik dan sepeda listrik. Pada aplikasi tersebut rel tegangan yang banyak digunakan adalah di level 48 V. Pengendali arus sangat dibutuhkan dalam proses pengisian baterai. Untuk merealisasikan kendali arus berbasis PID pada dc-dc converter, cenderung lebih sulit. Oleh karena itu, pada penelitian ini, kendali arus yang digunakan adalah pengendali hysteresis atau umumnya lebih dikenal sebagai bang-bang comparator. Keunggulan kendali ini adalah memiliki respon yang cepat serta secara alami mengatasi masalah hubung singkat pada terminal keluaran. Pengendali arus diimplementasikan pada board arduino mega. Pada penelitian ini, rangkaian dc-dc converter dengan jenis buck telah berhasil diimplementasikan beserta dengan dengan arus keluaran terkendali. Pengendali arus juga dapat diimplementasikan pada board arduino mega 2560. Berdasarkan hasil eksperimen, efisiensi tertinggi yang mampu dicapai pada konverter ini adalah 88 %. Pengisian pada baterai dengan sistem tegangan 48 V berjalan sesuai dengan mode constant current.
Immunolab: Combining targeted real-world data with advanced analytics to generate evidence at scale in immunology
Authors: Hamelin Bernard , Rowe Paul , Molony Cliona , Kruger Mark , LoCasale Robert , Khan Asif H. , Jacob-Nara Juby , Jacob Dan
Published: November 2022

Real-world evidence (RWE) has traditionally been used by regulatory or payer authorities to inform disease burden, background risk, or conduct post-launch pharmacovigilance, but in recent years RWE has been increasingly used to inform regulatory decision-making. However, RWE data sources remain fragmented, and datasets are disparate and often collected inconsistently. To this end, we have constructed an RWE-generation platform, Immunolab, to facilitate data-driven insights, hypothesis generation and research in immunological diseases driven by type 2 inflammation. Immunolab leverages a large, anonymized patient cohort from the Optum electronic health record and claims dataset containing over 17 million patient lives. Immunolab is an interactive platform that hosts three analytical modules: the Patient Journey Mapper, to describe the drug treatment patterns over time in patient cohorts; the Switch Modeler, to model treatment switching patterns and identify its drivers; and the Head-to-Head Simulator, to model the comparative effectiveness of treatments based on relevant clinical outcomes. The Immunolab modules utilize various analytic methodologies including machine learning algorithms for result generation which can then be presented in various formats for further analysis and interpretation.
Prey fear of a specialist predator in a tri-trophic food web can eliminate the superpredator
Authors: Fakhry Nabaa Hassain , Naji Raid Kamel , Smith? Stacey R. , Haque Mainul
Published: November 2022

We propose an intraguild predation ecological system consisting of a tri-trophic food web with a fear response for the basal prey and a Lotka–Volterra functional response for predation by both a specialist predator (intraguild prey) and a generalist predator (intraguild predator), which we call the superpredator. We prove the positivity, existence, uniqueness, and boundedness of solutions, determine all equilibrium points, prove global stability, determine local bifurcations, and illustrate our results with numerical simulations. An unexpected outcome of the prey's fear of its specialist predator is the potential eradication of the superpredator.
Analysis of influencing factors for prognosis of patients with ventricular septal perforation: A single-center retrospective study
Authors: Duan Ming-Xuan , Zhao Xi , Li Shao-Lin , Tao Jun-Zhong , Li Bo-Yan , Meng Xin-Guo , Dai Dong-Pu , Lu Yan-Yu , Yue Zhen-Zhen , Du Yang , Rui Zi-Ao , Pang Shuo , Zhou Yuan-Hang , Miao Guang-Rui , Bai Lin-Peng , Zhang Qing-Yang , Zhao Xiao-Yan
Published: November 2022

Background

Ventricular septal rupture (VSR) is a type of cardiac rupture, usually complicated by acute myocardial infarction (AMI), with a high mortality rate and often poor prognosis. The aim of our study was to investigate the factors influencing the long-term prognosis of patients with VSR from different aspects, comparing the evaluation performance of the Gensini score, Sequential Organ Failure Assessment (SOFA) score and European Heart Surgery Risk Assessment System II (EuroSCORE II) score systems.

Methods

This study retrospectively enrolled 188 patients with VSR between Dec 9, 2011 and Nov 21, 2021at the First Affiliated Hospital of Zhengzhou University. All patients were followed up until Jan 27, 2022 for clinical data, angiographic characteristics, echocardiogram outcomes, intraoperative, postoperative characteristics and major adverse cardiac events (MACEs) (30-day mortality, cardiac readmission). Cox proportional hazard regression analysis was used to explore the predictors of long-term mortality.

Results

The median age of 188 VSR patients was 66.2 ± 9.1 years and 97 (51.6%) were males, and there were 103 (54.8%) patients in the medication group, 34 (18.1%) patients in the percutaneous transcatheter closure (TCC) group, and 51 (27.1%) patients in the surgical repair group. The average follow-up time was 857.4 days. The long-term mortality of the medically managed group, the percutaneous TCC group, and the surgical repair group was 94.2, 32.4, and 35.3%, respectively. Whether combined with cardiogenic shock (OR 0.023, 95% CI 0.001–0.054, P = 0.019), NT-pro BNP level (OR 0.027, 95% CI 0.002–0.34, P = 0.005), EuroSCORE II (OR 0.530, 95% CI 0.305–0.918, P = 0.024) and therapy group (OR 3.518, 95% CI 1.079–11.463, P = 0.037) were independently associated with long-term mortality in patients with VSR, and this seems to be independent of the therapy group. The mortality rate of surgical repair after 2 weeks of VSR was much lower than within 2 weeks (P = 0.025). The cut-off point of EuroSCORE II was determined to be 14, and there were statistically significant differences between the EuroSCORE II < 14 group and EuroSCORE II≥14 group (HR = 0.2596, 95%CI: 0.1800–0.3744, Logrank P < 0.001).

Conclusion

Patients with AMI combined with VSR have a poor prognosis if not treated surgically, surgical repair after 2 weeks of VSR is a better time. In addition, EuroSCORE II can be used as a scoring system to assess the prognosis of patients with VSR.
Influence of COVID-19 restrictions on student satisfaction with undergraduate pathology teaching in an Australian University
Authors: Rodrigo Chaturaka , Herbert Cristan , Saunders Darren , Thomas Shane , Polly Patsie
Published: November 2022

Introduction

Almost three years into the COVID-19 pandemic, it is important to reflect on student perceptions of online teaching, and more specifically, if mobility restrictions imposed as public health measures significantly influenced how students perceived online teaching. The aim of this study was to investigate if student perceptions of teaching quality of undergraduate courses would differ when evaluated at times of increasing or relaxing COVID-related mobility restrictions.

Methods

We compared student feedback for two third-year undergraduate Pathology courses taken as part of a Bachelor of Medical Sciences / Science degree in an Australian University from 2019 to 2021. Quantitative feedback on five domains (overall satisfaction, belongingness within a learning community, satisfaction with assessments, adequacy of learning resources, satisfaction with teacher feedback) were categorized into groups based on calendar year or prevailing COVID restrictions (times with no, increasing or relaxing restrictions), and compared. There were no significant changes to the course content during this time, but face-to-face teaching in 2019 changed to predominantly online teaching in 2020 and 2021.

Results

Feedback scores were significantly better (p < 0.017) at times of increased COVID restrictions compared to times with relaxing COVID restrictions across all five domains assessed. Interestingly, when grouped by calendar years (instead of prevailing COVID restrictions), there were mostly no significant differences in the feedback scores, despite the shift to online teaching in 2020/21.

Conclusion

At times of increasing mobility restrictions, students may appreciate the consistency offered by well-structured online teaching but when restrictions are relaxed, online-only teaching may not meet their expectations. The teaching methods need to adapt to prevailing situation by focusing on more hands on and face-to-face teaching when circumstances allow it.
One teacher educator’s strategies for encouraging reflective practice
Authors: Russell Tom
Published: November 2022

Teaching reflective practice to beginning teachers requires significant changes in a teacher educator’s implicit assumptions about how individuals learn to teach. Teaching reflective practice also requires significant changes in a teacher educator’s teaching practices. The familiar teach-them-theory-and-then-let-them-practice approach assumes that learning is complete before practice begins. In contrast, reflective practice in professions involves learning from firsthand experience and recognizes that the process of learning theory and research is incomplete before personal practice begins. Teaching reflective practice also requires recognizing that terms such asreflectandreflectionare everyday words with multiple meanings and uses and little direct connection toreflective practice. The following argument, grounded in self-study methodology, describes indirect strategies for encouraging reflective practice. These strategies include an extended writing assignment focused on professional learning, teaching how to learn from personal experience, the unrecognized power of listening, and tickets out of class as listening and fostering metacognition. The argument closes with a summary of suggested strategies for encouraging reflective practice by those learning how to teach.
Does aerobic exercise reduce NASH and liver fibrosis in patients with non-alcoholic fatty liver disease? A systematic literature review and meta-analysis
Authors: Houttu Veera , Bouts Julia , Vali Yasaman , Daams Joost , Grefhorst Aldo , Nieuwdorp Max , Holleboom Adriaan G.
Published: November 2022

Background

Exercise is an effective strategy for the prevention and regression of hepatic steatosis in patients with non-alcoholic fatty liver disease (NAFLD), but it is unclear whether it can reduce advanced stages of NAFLD, i.e., steatohepatitis and liver fibrosis. Furthermore, it is not evident which modality of exercise is optimal to improve/attenuate NAFLD.

Objectives

The aim is to systematically review evidence for the effect of aerobic exercise (AE) on NAFLD, in particular non-alcoholic steatohepatitis (NASH) and liver fibrosis.

Methods

A systematic literature search was conducted in Medline and Embase. Studies were screened and included according to predefined criteria, data were extracted, and the quality was assessed by Cochrane risk of bias tools by two researchers independently according to the protocol registered in the PROSPERO database (CRD42021270059). Meta-analyses were performed using a bivariate random-effects model when there were at least three randomized intervention studies (RCTs) with similar intervention modalities and outcome.

Results

The systematic review process resulted in an inclusion a total of 24 studies, 18 RCTs and six non-RCTs, encompassing 1014 patients with NAFLD diagnosed by histological or radiological findings. Studies were grouped based on the type of AE: moderate-intensity continuous training (MICT) and high-intensity interval training (HIIT). A total of twelve meta-analyses were conducted. Compared to controls, MICT resulted in a mean difference (MD) in the NAFLD biomarkers alanine transaminase (ALT) and aspartate aminotransferase (AST) of -3.59 (CI: -5.60, -1.59, p<0.001) and -4.05 (CI: -6.39, -1.71, p<0.001), respectively. HIIT resulted in a MD of -4.31 (95% CI: -9.03, 0.41, p=0.07) and 1.02 (95% CI: -6.91, 8.94, p=0.8) for ALT and AST, respectively. Moreover, both AE types compared to controls showed a significantly lower magnetic resonance spectroscopy (MRS) determined liver fat with a MD of -5.19 (95% CI: -7.33, -3.04, p<0.001) and -3.41 (95% CI: -4.74, -2.08, p<0.001), for MICT and HIIT respectively. MICT compared to controls resulted in a significantly higher cardiorespiratory fitness (MD: 4.43, 95% CI: 0.31, 8.55, p=0.03).

Conclusion

Liver fat is decreased by AE with a concomitant decrease of liver enzymes. AE improved cardiorespiratory fitness. Further studies are needed to elucidate the impact of different types of AE on hepatic inflammation and fibrosis.

Systematic Review Registration

https://www.crd.york.ac.uk/prospero/, identifier (CRD42021270059).
Secular increase in the prevalence of gestational diabetes and its associated adverse pregnancy outcomes from 2014 to 2021 in Hebei province, China
Authors: Tian Mei-Ling , Du Li-Yan , Ma Guo-Juan , Zhang Ting , Ma Xu-Yuan , Zhang Ying-Kui , Tang Zeng-Jun
Published: November 2022

Objective

We aimed to investigate the secular prevalence of gestational diabetes mellitus (GDM) and evaluate its adverse pregnancy outcomes among pregnant women in Hebei province, China.

Methods

We analyzed the data from the monitoring information management system for pregnant women in 22 hospitals of Hebei province, China. In this study, 366,212 individuals with singleton live births from 2014 to 2021 were included, of whom 25,995 were diagnosed with gestational diabetes. We described the incidence of common complications and further analyzed the clinical characteristics in GDM patients and the relationship between GDM and adverse pregnancy outcomes.

Results

The top 3 pregnancy complications in Hebei province are anemia, gestational hypertension, and GDM. The average incidence of GDM was 7.10% (25,995/366,212). The incidence rate of GDM significantly increased from 2014 to 2021 (χ2trend = 7,140.663, P < 0.001). The top 3 regions with GDM incidence were Baoding (16.60%), Shijiazhuang (8.00%), and Tangshan (3.80%). The incidence of GDM in urban pregnant women (10.6%) is higher than that in rural areas (3.7%).The difference between the GDM and Non-GDM groups was statistically significant in terms of maternal age, gravidity, parity, education level, and incidence of pregnancy complications (gestational hypertension, heart diseases, and anemia) (P < 0.05). GDM individuals were at significantly increased risk of most assessed adverse pregnancy outcomes, including premature delivery, Cesarean delivery, uterine inertia, neonatal intensive care unit (NICU) admission, Apgar (activity-pulse-grimace-appearance-respiration) score at 1 min, and macrosomia (P < 0.05). The multivariate logistic regression analysis showed that GDM was an independent risk factor in terms of premature birth, Cesarean delivery, uterine inertia, placental abruption, NICU admission, and macrosomia.

Conclusion

The risk of adverse pregnancy outcome in pregnant women with GDM is significantly increased. In order to reduce the occurrence of adverse pregnancy outcomes, effective interventions are needed.
Blue carbon ecosystem monitoring using remote sensing reveals wetland restoration pathways
Authors: Lanceman Dana , Sadat-Noori Mahmood , Gaston Troy , Drummond Christopher , Glamore William
Published: November 2022

In an era of climate and biodiversity crises, ecosystem rehabilitation is critical to the ongoing wellbeing of humans and the environment. Coastal ecosystem rehabilitation is particularly important, as these ecosystems sequester large quantities of carbon (known in marine ecosystems as “blue carbon”) thereby mitigating climate change effects while also providing ecosystem services and biodiversity benefits. The recent formal accreditation of blue carbon services is producing a proliferation of rehabilitation projects, which must be monitored and quantified over time and space to assess on-ground outcomes. Consequently, remote sensing techniques such as drone surveys, and machine learning techniques such as image classification, are increasingly being employed to monitor wetlands. However, few projects, if any, have tracked blue carbon restoration across temporal and spatial scales at an accuracy that could be used to adequately map species establishment with low-cost methods. This study presents an open-source, user-friendly workflow, using object-based image classification and a random forest classifier in Google Earth Engine, to accurately classify 4 years of multispectral and photogrammetrically derived digital elevation model drone data at a saltmarsh rehabilitation site on the east coast of Australia (Hunter River estuary, NSW). High classification accuracies were achieved, with >90% accuracy at 0.1 m resolution. At the study site, saltmarsh colonised most suitable areas, increasing by 142% and resulting in 56 tonnes of carbon sequestered, within a 4-year period, providing insight into blue carbon regeneration trajectories. Saltmarsh growth patterns were species-specific, influenced by species’ reproductive and dispersal strategies. Our findings suggested that biotic factors and interactions were important in influencing species’ distributions and succession trajectories. This work can help improve the efficiency and effectiveness of restoration planning and monitoring at coastal wetlands and similar ecosystems worldwide, with the potential to apply this approach to other types of remote sensing imagery and to calculate other rehabilitation co-benefits. Importantly, the method can be used to calculate blue carbon habitat creation following tidal restoration of coastal wetlands.
Genetic structure and demographic history of Northern Han people in Liaoning Province inferred from genome-wide array data
Authors: Zhou Jingbin , Zhang Xianpeng , Li Xin , Sui Jie , Zhang Shuang , Zhong Hua , Zhang Qiuxi , Zhang Xiaoming , Huang He , Wen Youfeng
Published: November 2022

In this study, we used typical and advanced population genetic analysis methods [principal component analysis (PCA), ADMIXTURE, FST, f3-statistics, f4-statistics, qpAdm/qpWave, qpGraph, ALDER (Admixture-induced Linkage Disequilibrium for Evolutionary Relationships) and TreeMix] to explore the genetic structure of 80 Han individuals from four different cities in Liaoning Province and reconstruct their demographic history based on the newly generated genome-wide data. We found that Liaoning Han people have genetic similarities with other northern Han people (Shandong, Henan, and Shanxi) and Liaoning Manchu people. Millet farmers in the Yellow River Basin (YRB) and the West Liao River Basin (WLRB) (57–98%) and hunter-gatherers in the Mongolian Plateau (MP) and the Amur River Basin (ARB) (40–43%) are the main ancestral sources of the Liaoning Han people. Our study further supports the “northern origin hypothesis”; YRB-related ancestry accounts for 83–98% of the genetic makeup of the Liaoning Han population. There are clear genetic influences of northern East Asian populations in the Liaoning Han people, ancient Northeast Asian-related ancestry is another dominant ancestral component, and large-scale population admixture has happened between Tungusic Manchu people and Han people. There are genetic differences among the Liaoning Han people, and we found that these differences are associated with different migration routes of Hans during the “Chuang Guandong” period in historical records.
Whole genome sequencing of the fast-swimming Southern bluefin tuna (Thunnus maccoyii)
Authors: Zhao Xiaomeng , Huang Yu , Bian Chao , You Xinxin , Zhang Xinhui , Chen Jieming , Wang Min , Hu Cancan , Xu Yun , Xu Junmin , Shi Qiong
Published: November 2022

The economically important Southern bluefin tuna (Thunnus maccoyii) is a world-famous fast-swimming fish, but its genomic information is limited. Here, we performed whole genome sequencing and assembled a draft genome for Southern bluefin tuna, aiming to generate useful genetic data for comparative functional prediction. The final genome assembly is 806.54 Mb, with scaffold and contig N50 values of 3.31 Mb and 67.38 kb, respectively. Genome completeness was evaluated to be 95.8%. The assembled genome contained 23,403 protein-coding genes and 236.1 Mb of repeat sequences (accounting for 29.27% of the entire assembly). Comparative genomics analyses of this fast-swimming tuna revealed that it had more than twice as many hemoglobin genes (18) as other relatively slow-moving fishes (such as seahorse, sunfish, and tongue sole). These hemoglobin genes are mainly localized in two big clusters (termed as “MNˮ and “LAˮ respectively), which is consistent with other reported fishes. However, Thr39 of beta-hemoglobin in the MN cluster, conserved in other fishes, was mutated as cysteine in tunas including the Southern bluefin tuna. Since hemoglobins are reported to transport oxygen efficiently for aerobic respiration, our genomic data suggest that both high copy numbers of hemoglobin genes and an adjusted function of the beta-hemoglobin may support the fast-swimming activity of tunas. In summary, we produced a primary genome assembly and predicted hemoglobin-related roles for the fast-swimming Southern bluefin tuna.
A novel senescence-related lncRNA signature that predicts prognosis and the tumor microenvironment in patients with lung adenocarcinoma
Authors: Fang Xueying , Huang Enmin , Xie Xiaopeng , Yang Kai , Wang Shuqian , Huang Xiaoqing , Song Mei
Published: November 2022

Background: Cellular senescence has recently been considered a new cancer hallmark. However, the factors regulating cellular senescence have not been well characterized. The aim of this study is to identify long non-coding RNAs (lncRNAs) associated with senescence and prognosis in patients with lung adenocarcinoma (LUAD).

Methods: Using RNA sequence data from the Cancer Genome Atlas Lung Adenocarcinoma (TCGA-LUAD) and senescence genes from the CellAge database, a subset of senescence-related lncRNAs was first identified. Then, using univariate and multivariate Cox regression analyses, a senescence lncRNA signature (LUADSenLncSig) associated with LUAD prognosis was developed. Based on the median LUADSenLncSig risk score, LUAD patients were divided into high-risk and low-risk groups. Kaplan-Meier analysis was used to compare the overall survival (OS) in the high- and low-risk score subgroups. Differences in Gene Set Enrichment Analysis (GSEA), immune infiltration, tumor mutation burden (TMB), tumor immune dysfunction and exclusion (TIDE) module score, chemotherapy, and targeted therapy selection were also compared between the high-risk and low-risk groups.

Results: A prognostic risk model was obtained consisting of the following nine senescence-related lncRNAs: LINC01116, AC005838.2, SH3PXD2A-AS1, VIMS-AS1, SH3BP5-AS1, AC092279.1, AC026355.1, AC027020.2, and LINC00996. The LUADSenLncSig high-risk group was associated with poor OS (hazard ratio = 1.17, 95% confidence interval = 1.102–1.242; p < 0.001). The accuracy of the model was further supported based on receiver operating characteristic (ROC), principal component analysis (PCA), and internal validation cohorts. In addition, a nomogram was developed consisting of LUADSenLncSig for LUAD prognosis, which is consistent with the actual probability of OS. Furthermore, immune infiltration analysis showed the low-risk group had a stronger anti-tumor immune response in the tumor microenvironment. Notably, the levels of immune checkpoint genes such as CTLA-4, PDCD-1, and CD274, and the TIDE scores were significantly higher in the low-risk subgroups than in high-risk subgroups (p < 0.001). This finding indicates the LUADSenLncSig can potentially predict immunotherapy efficacy.

Conclusion: In this study, a lncRNA signature, LUADSenLncSig, that has dual functions of senescence phenotype identification and prognostic prediction as well as the potential to predict the LUAD response to immunotherapy was developed.
Exploration of blood−derived coding and non-coding RNA diagnostic immunological panels for COVID-19 through a co-expressed-based machine learning procedure
Authors: Zarei Ghobadi Mohadeseh , Emamzadeh Rahman , Teymoori-Rad Majid , Afsaneh Elaheh
Published: November 2022

Severe acute respiratory syndrome coronavirus 2 (SARS- CoV-2) is the causative virus of the pandemic coronavirus disease 2019 (COVID-19). Evaluating the immunological factors and other implicated processes underlying the progression of COVID-19 is essential for the recognition and then the design of efficacious therapies. Therefore, we analyzed RNAseq data obtained from PBMCs of the COVID-19 patients to explore coding and non-coding RNA diagnostic immunological panels. For this purpose, we integrated multiple RNAseq data and analyzed them overall as well as by considering the state of disease including severe and non-severe conditions. Afterward, we utilized a co-expressed-based machine learning procedure comprising weighted-gene co-expression analysis and differential expression gene as filter phase and recursive feature elimination-support vector machine as wrapper phase. This procedure led to the identification of two modules containing 5 and 84 genes which are mostly involved in cell dysregulation and innate immune suppression, respectively. Moreover, the role of vitamin D in regulating some classifiers was highlighted. Further analysis disclosed the role of discriminant miRNAs including miR-197-3p, miR-150-5p, miR-340-5p, miR-122-5p, miR-1307-3p, miR-34a-5p, miR-98-5p and their target genes comprising GAN, VWC2, TNFRSF6B, and CHST3 in the metabolic pathways. These classifiers differentiate the final fate of infection toward severe or non-severe COVID-19. The identified classifier genes and miRNAs may help in the proper design of therapeutic procedures considering their involvement in the immune and metabolic pathways.
Poor knowledge and awareness of human papillomavirus and cervical cancer among adult females in rural Ghana
Authors: Ken-Amoah Sebastian , Blay Mensah Loretta Betty , Eliason Sebastian , Anane-Fenin Betty , Agbeno Evans Kofi , Essuman Mainprice Akuoko , Essien-Baidoo Samuel
Published: November 2022

Cervical cancer (CC) continues to be a global burden, and a disease of public health interest, especially in low- and middle-income countries. This study explored the knowledge and awareness of human papillomavirus (HPV) infection and CC and the risk-behavioural characteristics of an adult female population in rural Ghana. A total of 274 women, aged 21 years and above were recruited through multi-stage sampling. A questionnaire was used to obtain data on their socio-demographic and socio-economic characteristics and assess their knowledge and awareness of HPV and CC through face-to-face interviews. In all, 187 (68.2%) participants had poor knowledge of HPV and CC with 87 (31.8%) having good knowledge. Only 18.2% and 48.9% of them had prior awareness of HPV infection and CC, respectively. Their primary source of information was the broadcast media (TV and Radio stations) (60.1%). Regarding risky lifestyle behaviours, the mean age at sexual debut was 18.52 ± 2.39 years, 82.8% have had more than one lifetime sexual partner, 81.0% were multiparous with 51.5% using oral contraceptives. Women with higher educational attainment (cOR = 0.41; 95%CI = 0.20-0.81), monthly income (cOR = 0.31; 95%CI = 0.10-1.02), and lifetime sexual partners (aOR = 0.46; 95%CI = 0.21-1.00) were less likely to have poor CC and HPV knowledge compared to their counterparts. In conclusion, adult females in the rural population have limited knowledge and awareness of HPV infection and CC with low CC screening. Intensive educational campaigns should be organised and spearheaded on multiple platforms by the Ghana Health Service and civil societies to sensitise and educate women, in the rural parts of the country, on CC and its related preventive measures.
Social cohesion and self-governance arrangements among small-scale fisheries in Puerto Rico
Authors: Gómez-Andújar Nicolás X. , Gerkey Drew , Conway Flaxen , Watson James R.
Published: November 2022

In small-scale fisheries that suffer from weak formal governance, fishers use a variety of institutional strategies to self-govern. These self-governance arrangements may or may not be forms of collective action, yet they rely on informal institutions (such as norms of reciprocity) in generating social cohesion among users so that common rules can be agreed upon. Social network perspectives have been used to understand the cohesiveness of fishers operating with different gear types or in different communities, yet less is known about how social cohesion differs between fishers that operate through formal cooperative arrangements, such as fishing associations, and independent fishers who operate solely at the community level through informal cooperation. Using mixed methods, we compared the social cohesion of commercial fishers arising from information-sharing and work-support networks in four fishing communities of north-eastern Puerto Rico, as they coped with prolonged hurricane recovery and the COVID-19 pandemic. A series of exponential random graph models (ERGMs) were applied to untangle the drivers of cohesion emerging from fisher’s information-sharing patterns. Fisher’s communication was driven not just by operating at the same landing site or using the same primary gear type, but also by choosing to organize their fishing under the same self-governance arrangement. Specifically, fishers that were members of fishing associations mostly communicated among themselves, while independent fishers talked across arrangements, indicating the role of fishing associations in fostering social cohesion. However, associated fishers were less likely to receive information than independent fishers, suggesting the need for targeted communication strategies within each group. Moreover, independent fishers had more work support ties and a greater diversity of supporting actors across the supply chain, suggesting their need for higher levels of linking social capital. Multiple social factors explained whether fishers choose to be members of fishing associations or remain independent, including trust in leaders and agreement on membership rules. These findings highlight the importance of knowing the cohesiveness of cooperation networks among small-scale fishers.
Characterization of magnetorheological fluids based on capillary magneto-rheometer
Authors: Wen Mingfu , Du Yueqian , Liu Runduo , Li Zeqin , Rao Longshi , Xiao Hongwei , Ouyang Yi , Niu Xiaodong
Published: November 2022

Magnetorheological fluids (MRFs) are a class of smart magnetic controlled materials whose rheological properties can be controlled by a magnetic field. These materials have advantages of short response time, high dynamic range and low energy consumption. Due to their excellent properties, MRFs have a widely application potential in the field of impact mitigation. As the shear rate dependent viscosity of MRFs is astonishing for the shear thinning effect, thus it is crucial to study the rheological properties cross a wide range of shear rates for guiding the design and application of MRFs based adaptive impact absorbers. Commercial rotational rheometers are usually used to test the rheological properties of MRFs, but their range of measuring shear rates are limited. Commercial capillary rheometers are designed to measure the rheological behavior over a wide range of shear rates, but they’re usually lack of ability to measure with magnetic field. In order to study the rheological properties of MRFs under higher shear rate and applied magnetic field, a lab-made speed-controlled capillary magneto-rheometer is developed in the presnt work; The expressions for equivalent shear rate and apparent viscosity of MRFs under the dimensional constraint of the set-up are derived. In addition, the theoretical expression of shear rate of MRFs is modified by Rabinowitsch correction. Then, the rheological properties of three particle volume fractions (10%, 15%, and 20%)of MRFs with different magnetic field strengths (6 mT, 13 mT, 20 mT, and 25 mT)are tested and analyzed, and the rheological characteristic curves of MRFs with shear rate range of 101s−1–105s−1 are obtained with the normalized characterization using Mason number. According to the experimental results, the MRFs show an obvious shear thinning phenomenon as shear rate increases, and in the Mason number based normalized characterization, the curves of different particle volume fractions are collapse to a master curve.
Interfacial treatment-induced high-strength plant fiber/phenolic resin composite
Authors: Zhou Shaoqian , Chen Xueying , Huang Rixin , Lin Yi , Ye Xiaoxia
Published: November 2022

Plant straw fibers have been used widely in composite materials as reinforcement due to their advantages of low density, easy degradation, low cost, and abundance. However, the mechanical properties and water resistance of the as-prepared composites are limited due to the poor compatibility between plant straw fibers and polymer matrix. In this work, the rice straw fibers were pretreated with the silane coupling agent, lipase, and hydrothermal, and then blended with phenolic resin to prepare composites. The results showed that the pretreatment of the fibers can improve their compatibility with the resin matrix and improve the comprehensive mechanical properties and the thickness swelling of composites. When the mass ratio of fiber to resin is 1:1 and the hot-pressing condition is 160°C and 11 min, the static bending strength, elastic modulus, and thickness swelling of the phenolic resin/lipase-treatment rice straw fibers (PF/L-SF) composite reached 23.03 MPa and 2588.39 MPa, which increased by 32.9% and 30.5% compared with the unmodified fiber/phenolic resin composite (PF/SF).
Microbiome analysis reveals universal diagnostic biomarkers for colorectal cancer across populations and technologies
Authors: Zhang Huarong , Wu Junling , Ji Daihan , Liu Yijuan , Lu Shuting , Lin Zeman , Chen Ting , Ao Lu
Published: November 2022

The gut microbial dysbiosis is a risk of colorectal cancer (CRC) and some bacteria have been reported as potential markers for CRC diagnosis. However, heterogeneity among studies with different populations and technologies lead to inconsistent results. Here, we investigated six metagenomic profiles of stool samples from healthy controls (HC), colorectal adenoma (CA) and CRC, and six and four genera were consistently altered between CRC and HC or CA across populations, respectively. In FengQ cohort, which composed with 61 HC, 47 CA, and 46 CRC samples, a random forest (RF) model composed of the six genera, denoted as signature-HC, distinguished CRC from HC with an area under the curve (AUC) of 0.84. Similarly, another RF model composed of the four universal genera, denoted as signature-CA, discriminated CRC from CA with an AUC of 0.73. These signatures were further validated in five metagenomic sequencing cohorts and six independent 16S rRNA gene sequencing cohorts. Interestingly, three genera overlapped in the two models (Porphyromonas, Parvimonas and Peptostreptococcus) were with very low abundance in HC and CA, but sharply increased in CRC. A concise RF model on the three genera distinguished CRC from HC or CA with AUC of 0.87 and 0.67, respectively. Functional gene family analysis revealed that Kyoto Encyclopedia of Genes and Genomes Orthogroups categories which were significantly correlated with markers in signature-HC and signature-CA were mapped into pathways related to lipopolysaccharide and sulfur metabolism, which might be vital risk factors of CRC development. Conclusively, our study identified universal bacterial markers across populations and technologies as potential aids in non-invasive diagnosis of CRC.
Non-lactose fermenting Escherichia coli: Following in the footsteps of lactose fermenting E. coli high-risk clones
Authors: Mazumder Razib , Hussain Arif , Phelan Jody E. , Campino Susana , Haider S. M. Arefeen , Mahmud Araf , Ahmed Dilruba , Asadulghani Md , Clark Taane G. , Mondal Dinesh
Published: November 2022

Multi-resistant pathogenic strains of non-lactose fermenting Escherichia coli (NLF E. coli) are responsible for various intestinal and extraintestinal infections. Although several studies have characterised such strains using conventional methods, they have not been comprehensively studied at the genomic level. To address this gap, we used whole-genome sequencing (WGS) coupled with detailed microbiological and biochemical testing to investigate 17 NLF E. coli from a diagnostic centre (icddr,b) in Dhaka, Bangladesh. The prevalence of NLF E. coli was 10%, of which 47% (8/17) exhibited multi-drug resistant (MDR) phenotypes. All isolates (17/17) were confirmed as E. coli and could not ferment lactose sugar. WGS data analysis revealed international high-risk clonal lineages. The most prevalent sequence types (STs) were ST131 (23%), ST1193 (18%), ST12 (18%), ST501 (12%), ST167 (6%), ST73 (6%) and ST12 (6%). Phylogenetic analysis corroborated a striking clonal population amongst the studied NLF E. coli isolates. The predominant phylogroup detected was B2 (65%). The blaCTX-M-15 extended-spectrum beta-lactamase gene was present in 53% of isolates (9/17), whilst 64.7% (11/17) isolates were affiliated with pathogenic pathotypes. All extraintestinal pathogenic E. coli pathotypes demonstrated β-hemolysis. Our study underscores the presence of critical pathogens and MDR clones amongst non-lactose fermenting E. coli. We suggest that non-lactose fermenting E. coli be considered equally capable as lactose fermenting forms in causing intestinal and extraintestinal infections. Further, there is a need to undertake systematic, unbiased monitoring of predominant lineages amongst non-lactose fermenting E. coli that would help in better treatment and prevention strategies.
Redox signaling and metabolism in Alzheimer's disease
Authors: Holubiec M. I. , Gellert M. , Hanschmann E. M.
Published: November 2022

Reduction and oxidation reactions are essential for biochemical processes. They are part of metabolic pathways and signal transduction. Reactive oxygen species (ROS) as second messengers and oxidative modifications of cysteinyl (Cys) residues are key to transduce and translate intracellular and intercellular signals. Dysregulation of cellular redox signaling is known as oxidative distress, which has been linked to various pathologies, including neurodegeneration. Alzheimer's disease (AD) is a neurodegenerative pathology linked to both, abnormal amyloid precursor protein (APP) processing, generating Aβ peptide, and Tau hyperphosphorylation and aggregation. Signs of oxidative distress in AD include: increase of ROS (H2O2, O2•−), decrease of the levels or activities of antioxidant enzymes, abnormal oxidation of macromolecules related to elevated Aβ production, and changes in mitochondrial homeostasis linked to Tau phosphorylation. Interestingly, Cys residues present in APP form disulfide bonds that are important for intermolecular interactions and might be involved in the aggregation of Aβ. Moreover, two Cys residues in some Tau isoforms have been shown to be essential for Tau stabilization and its interaction with microtubules. Future research will show the complexities of Tau, its interactome, and the role that Cys residues play in the progression of AD. The specific modification of cysteinyl residues in redox signaling is also tightly connected to the regulation of various metabolic pathways. Many of these pathways have been found to be altered in AD, even at very early stages. In order to analyze the complex changes and underlying mechanisms, several AD models have been developed, including animal models, 2D and 3D cell culture, and ex-vivo studies of patient samples. The use of these models along with innovative, new redox analysis techniques are key to further understand the importance of the redox component in Alzheimer's disease and the identification of new therapeutic targets in the future.
Elevated cerebrospinal fluid protein levels associated with poor short-term outcomes after spinal cord stimulation in patients with disorders of consciousness
Authors: He Qiheng , Li Tianfei , Xiong Ying , Xia Xiaoyu , Dang Yuanyuan , Chen Xueling , Geng Xiaoli , He Jianghong , Yang Yi , Zhao Jizong
Published: November 2022

Background

Spinal cord stimulation (SCS) is a promising treatment for patients with disorders of consciousness (DoC); however, the laboratory examinations and different electrodes (permanent #39286 vs. temporary percutaneous #3777, Medtronic, USA) that are associated with postoperative outcomes are unclear. The study aims to study the association between the change in postoperative cerebrospinal fluid (CSF) protein level and improvement in consciousness after SCS in DoC patients and to explore whether different electrodes were associated with elevated CSF protein levels.

Materials and methods

A total of 66 DoC patients who received SCS treatment from December 2019 to December 2021 were retrospectively analyzed. Patients were grouped according to their elevated CSF protein level. The clinical characteristics of the patients and SCS stimulation parameters were compared. The preoperative sagittal diameter of the spinal canal is the distance from the midpoint of the posterior border of the vertebral body to the midpoint of the posterior wall of the spinal canal at the level of the superior border of C3. The postoperative sagittal diameter of the spinal canal is the distance from the midpoint of the posterior edge of the vertebral body to the anterior edge of the stimulation electrode. Patients with improved postoperative CRS-R scores greater than 3 or who progressed to the MCS + /eMCS were classified as the improved group and otherwise regarded as poor outcome.

Results

We found that more DoC patients had elevated CSF protein levels among those receiving SCS treatment with permanent electrodes than temporary percutaneous electrodes (P = 0.001), and elevated CSF protein levels were significantly associated with a reduced sagittal diameter (P = 0.044). In DoC patients receiving SCS treatment, we found that elevated CSF protein levels (P = 0.022) and preoperative diagnosis (P = 0.003) were significantly associated with poor outcomes at 3 months. Logistic regression analysis showed that elevated CSF protein levels were significantly associated with poor outcomes (OR 1.008, 95% CI 1.001–1.016, P = 0.032).

Conclusion

The results suggest that reducing the effect of electrode pads on anatomical changes may help improve the outcomes of DoC patients receiving SCS treatment. CSF protein levels are associated with poor postoperative outcomes and whether they are potential biomarkers in DoC patients receiving SCS treatment remain further exploration.
The value of circulating lymphocytic subpopulations in the diagnosis and repair of ischemic stroke patients with dizziness
Authors: Wang Yong , Huang Yichen , Li Sicheng , Lin Jixian , Liu Yang , Gao Yanqin , Zhao Jing
Published: November 2022

Background

To determine whether dizziness can contribute to stroke as a main cause still remains challenging. This study aims to explore clinical biomarkers in the identification of ischemic stroke patients from people with dizziness and the prediction of their long-term recovery.

Methods

From January 2018 to June 2019, 21 ischemic stroke patients with a main complaint of dizziness, 84 non-stroke dizziness patients and 87 healthy volunteers were recruited in this study. Then, their peripheral blood samples were collected, and the percentages of circulating lymphocytes T cells, CD4+ T cells, CD8+ T cells, T−/− cells (DNTs), CD4+ regulatory T cells (Tregs), CD8+ Tregs, B cells and regulatory B cells (Bregs) were examined to identify biomarkers with clinical value.

Results

According to our data, a significant difference in the DNTs proportion was detected between non-stroke dizziness and ischemic stroke patients with dizziness (p = 0.0009). The Bregs proportion in ischemic stroke patients with dizziness was lower than that in non-stroke dizziness patients (p = 0.035). In addition, the percentage of Bregs and DNTs within lymphocytes in patients’ peripheral blood exhibited a significant negative correlation with stroke occurrence (Bregs, p = 0.039; DNTs, p = 0.046). Moreover, the Bregs and DNTs within lymphocytes were negatively related to participants’ age, while presented a weak relationship with clinical risks like smoking, hypertension, and diabetes. Then, area under the receiver operating characteristic curve (AUC) of Bregs and DNTs together was 0.768, the risk factors and Bregs or DNTs ranged from 0.795 and 0.792, respectively, and the AUC value of risk factors, Bregs and DNTs combination was further increased to 0.815. Furthermore, the Bregs percentage within lymphocytes at admission was also a potential predictor of repair at discharge and the following 3 months.

Conclusion

Bregs and DNTs could be the clinical biomarkers together in the identification of ischemic stroke patients from people with dizziness.