bluuebunny/crossref_metadata_2025 huggingface dataset:
Genome-Wide Association Mapping of Prostrate/Erect Growth Habit in Winter Durum Wheat
Authors: Marone Daniela , Rodriguez Monica , Saia Sergio , Papa Roberto , Rau Domenico , Pecorella Ivano , Laidò Giovanni , Pecchioni Nicola , Lafferty Julia , Rapp Matthias , Longin Friedrich H. , De Vita Pasquale
Published: January 2020

By selecting for prostrate growth habit of the juvenile phase of the cycle, durum wheat cultivars could be developed with improved competitive ability against weeds, and better soil coverage to reduce the soil water lost by evaporation. A panel of 184 durum wheat (Triticum turgidum subsp. durum) genotypes, previously genotyped with DArT-seq markers, was used to perform association mapping analysis of prostrate/erect growth habit trait and to identify candidate genes. Phenotypic data of plant growth habit were recorded during three consecutive growing seasons (2014–2016), two different growth conditions (field trial and greenhouse) and two sowing periods (autumn and spring). Genome-wide association study revealed significant marker-trait associations, twelve of which were specific for a single environment/year, 4 consistent in two environments, and two MTAs for the LSmeans were identified across all environments, on chromosomes 2B and 5A. The co-localization of some MTAs identified in this study with known vernalization and photoperiod genes demonstrated that the sensitivity to vernalization and photoperiod response are actually not only key components of spring/winter growth habit, but they play also an important role in defining the magnitude of the tiller angle during the tillering stage. Many zinc-finger transcription factors, such as C2H2 or CCCH-domain zinc finger proteins, known to be involved in plant growth habit and in leaf angle regulation were found as among the most likely candidate genes. The highest numbers of candidate genes putatively related to the trait were found on chromosomes 3A, 4B, 5A and 6A. Moreover, a bioinformatic approach has been considered to search for functional ortholog genes in wheat by using the sequence of rice and barley tiller angle-related genes. The information generated could be used to improve the understanding of the mechanisms that regulate the prostrate/erect growth habit in wheat and the adaptive potential of durum wheat under resource-limited environmental conditions.
Characterization in Dual Activation by Oxaliplatin, a Platinum-Based Chemotherapeutic Agent of Hyperpolarization-Activated Cation and Electroporation-Induced Currents
Authors: Chang Wei-Ting , Gao Zi-Han , Li Shih-Wei , Liu Ping-Yen , Lo Yi-Ching , Wu Sheng-Nan
Published: January 2020

Oxaliplatin (OXAL) is regarded as a platinum-based anti-neoplastic agent. However, its perturbations on membrane ionic currents in neurons and neuroendocrine or endocrine cells are largely unclear, though peripheral neuropathy has been noted during its long-term administration. In this study, we investigated how the presence of OXAL and other related compounds can interact with two types of inward currents; namely, hyperpolarization-activated cation current (Ih) and membrane electroporation-induced current (IMEP). OXAL increased the amplitude or activation rate constant of Ih in a concentration-dependent manner with effective EC50 or KD values of 3.2 or 6.4 μM, respectively, in pituitary GH3 cells. The stimulation by this agent of Ih could be attenuated by subsequent addition of ivabradine, protopine, or dexmedetomidine. Cell exposure to OXAL (3 μM) resulted in an approximately 11 mV rightward shift in Ih activation along the voltage axis with minimal changes in the gating charge of the curve. The exposure to OXAL also effected an elevation in area of the voltage-dependent hysteresis elicited by long-lasting triangular ramp. Additionally, its application resulted in an increase in the amplitude of IMEP elicited by large hyperpolarization in GH3 cells with an EC50 value of 1.3 μM. However, in the continued presence of OXAL, further addition of ivabradine, protopine, or dexmedetomidine always resulted in failure to attenuate the OXAL-induced increase of IMEP amplitude effectively. Averaged current-voltage relation of membrane electroporation-induced current (IMEP) was altered in the presence of OXAL. In pituitary R1220 cells, OXAL-stimulated Ih remained effective. In Rolf B1.T olfactory sensory neurons, this agent was also observed to increase IMEP in a concentration-dependent manner. In light of the findings from this study, OXAL-mediated increases of Ih and IMEP may coincide and then synergistically act to increase the amplitude of inward currents, raising the membrane excitability of electrically excitable cells, if similar in vivo findings occur.
Correction: Fisher, Aron B., et al. A Peptide Inhibitor of NADPH Oxidase (NOX2) Activation Markedly Decreases Mouse Lung Injury and Mortality Following Administration of Lipopolysaccharide (LPS). Int. J. Mol. Sci. 2019, 20, 2395
Authors: Fisher Aron B. , Dodia Chandra , Chatterjee Shampa , Feinstein Sheldon I.
Published: January 2020

The authors wish to make the following corrections to our previously published paper [...]
Sirtuin 3 Activation by Honokiol Decreases Unilateral Ureteral Obstruction-Induced Renal Inflammation and Fibrosis via Regulation of Mitochondrial Dynamics and the Renal NF-κB-TGF-β1/Smad Signaling Pathway
Authors: Quan Yi , Park Woong , Jin Jixiu , Kim Won , Park Sung Kwang , Kang Kyung Pyo
Published: January 2020

Renal fibrosis is a common feature of all progressive chronic kidney diseases. Sirtuin 3 (SIRT3) is one of the mitochondrial sirtuins, and plays a role in the regulation of mitochondrial biogenesis, oxidative stress, fatty acid metabolism, and aging. Recently, honokiol (HKL), as a pharmaceutical SIRT3 activator, has been observed to have a protective effect against pressure overload-induced cardiac hypertrophy by increasing SIRT3 activity. In this study, we investigated whether HKL, as a SIRT3 activator, also has protective effects against unilateral ureteral obstruction (UUO)-induced renal tubulointerstitial fibrosis through SIRT3-dependent regulation of mitochondrial dynamics and the nuclear factor-κB (NF-κB)/transforming growth factor-β1 (TGF-β1)/Smad signaling pathway. We found that HKL decreased the UUO-induced increase in tubular injury and extracellular matrix (ECM) deposition in mice. HKL also decreased myofibroblast activation and proliferation in UUO kidneys and NRK-49F cells. Finally, we showed that HKL treatment decreased UUO-induced mitochondrial fission and promoted mitochondrial fusion through SIRT3-dependent effects. In conclusion, activation of SIRT3 via HKL treatment might have beneficial effects on UUO-induced renal fibrosis through SIRT3-dependent regulation of mitochondrial dynamics and the NF-κB/TGF-β1/Smad signaling pathway.
Tuning Water Networks via Ionic Liquid/Water Mixtures
Authors: Verma Archana , Stoppelman John P. , McDaniel Jesse G.
Published: January 2020

Water in nanoconfinement is ubiquitous in biological systems and membrane materials, with altered properties that significantly influence the surrounding system. In this work, we show how ionic liquid (IL)/water mixtures can be tuned to create water environments that resemble nanoconfined systems. We utilize molecular dynamics simulations employing ab initio force fields to extensively characterize the water structure within five different IL/water mixtures: [BMIM + ][BF 4 − ], [BMIM + ][PF 6 − ], [BMIM + ][OTf − ], [BMIM + ][NO 3 − ] and [BMIM + ][TFSI − ] ILs at varying water fraction. We characterize water clustering, hydrogen bonding, water orientation, pairwise correlation functions and percolation networks as a function of water content and IL type. The nature of the water nanostructure is significantly tuned by changing the hydrophobicity of the IL and sensitively depends on water content. In hydrophobic ILs such as [BMIM + ][PF 6 − ], significant water clustering leads to dynamic formation of water pockets that can appear similar to those formed within reverse micelles. Furthermore, rotational relaxation times of water molecules in supersaturated hydrophobic IL/water mixtures indicate the close-connection with nanoconfined systems, as they are quantitatively similar to water relaxation in previously characterized lyotropic liquid crystals. We expect that this physical insight will lead to better design principles for incorporation of ILs into membrane materials to tune water nanostructure.
Chemotherapeutic Stress Influences Epithelial–Mesenchymal Transition and Stemness in Cancer Stem Cells of Triple-Negative Breast Cancer
Authors: Li Xiao , Strietz Juliane , Bleilevens Andreas , Stickeler Elmar , Maurer Jochen
Published: January 2020

Triple-negative breast cancer (TNBC) is a subtype of breast cancer characterized by the absence of estrogen and progesterone receptors (ER, PR) and lacking an overexpression of human epidermal growth factor receptor 2 (HER2). Apart from this lack of therapeutic targets, TNBC also shows an increased capacity for early metastasis and therapy resistance. Currently, many TNBC patients receive neoadjuvant chemotherapy (NACT) upon detection of the disease. With TNBC likely being driven at least in part by a cancer stem-like cell type, we wanted to evaluate the response of primary cancer stem cells (CSCs) to standard chemotherapeutics. Therefore, we set up a survival model using primary CSCs to mimic tumor cells in patients under chemotherapy. Breast cancer stem cells (BCSCs) were exposed to chemotherapeutics with a sublethal dose for six days. Surviving cells were allowed to recover in culture medium without chemotherapeutics. Surviving and recovered cells were examined in regard to proliferation, migratory capacity, sphere forming capacity, epithelial–mesenchymal transition (EMT) factor expression at the mRNA level, and cancer-related microRNA (miRNA) profile. Our results indicate that chemotherapeutic stress enhanced sphere forming capacity of BCSCs, and changed cell morphology and EMT-related gene expression at the mRNA level, whereas the migratory capacity was unaffected. Six miRNAs were identified as potential regulators in this process.
Impact of the Sensory and Sympathetic Nervous System on Fracture Healing in Ovariectomized Mice
Authors: Niedermair Tanja , Straub Rainer H. , Brochhausen Christoph , Grässel Susanne
Published: January 2020

The peripheral nervous system modulates bone repair under physiological and pathophysiological conditions. Previously, we reported an essential role for sensory neuropeptide substance P (SP) and sympathetic nerve fibers (SNF) for proper fracture healing and bone structure in a murine tibial fracture model. A similar distortion of bone microarchitecture has been described for mice lacking the sensory neuropeptide α-calcitonin gene-related peptide (α-CGRP). Here, we hypothesize that loss of SP, α-CGRP, and SNF modulates inflammatory and pain-related processes and also affects bone regeneration during fracture healing under postmenopausal conditions. Intramedullary fixed femoral fractures were set to 28 days after bilateral ovariectomy (OVX) in female wild type (WT), SP-, α-CGRP-deficient, and sympathectomized (SYX) mice. Locomotion, paw withdrawal threshold, fracture callus maturation and numbers of TRAP-, CD4-, CD8-, F4/80-, iNos-, and Arg1-positive cells within the callus were analyzed. Nightly locomotion was reduced in unfractured SP-deficient and SYX mice after fracture. Resistance to pressure was increased for the fractured leg in SP-deficient mice during the later stages of fracture healing, but was decreased in α-CGRP-deficient mice. Hypertrophic cartilage area was increased nine days after fracture in SP-deficient mice. Bony callus maturation was delayed in SYX mice during the later healing stages. In addition, the number of CD 4-positive cells was reduced after five days and the number of CD 8-positive cells was additionally reduced after 21 days in SYX mice. The number of Arg1-positive M2 macrophages was higher in α-CGRP-deficient mice five days after fracture. The alkaline phosphatase level was increased in SYX mice 16 days after fracture. Absence of α-CGRP appears to promote M2 macrophage polarization and reduces the pain threshold, but has no effect on callus tissue maturation. Absence of SP reduces locomotion, increases the pain-threshold, and accelerates hypertrophic callus tissue remodeling. Destruction of SNF reduces locomotion after fracture and influences bony callus tissue remodeling during the later stages of fracture repair, whereas pain-related processes are not affected.
Molecular Changes Concomitant with Vascular System Development in Mature Galls Induced by Root-Knot Nematodes in the Model Tree Host Populus tremula × P. alba
Authors: Baldacci-Cresp Fabien , Behr Marc , Kohler Annegret , Badalato Nelly , Morreel Kris , Goeminne Geert , Mol Adeline , de Almeida Engler Janice , Boerjan Wout , El Jaziri Mondher , Baucher Marie
Published: January 2020

One of the most striking features occurring in the root-knot nematode Meloidogyne incognita induced galls is the reorganization of the vascular tissues. During the interaction of the model tree species Populus and M. incognita, a pronounced xylem proliferation was previously described in mature galls. To better characterise changes in expression of genes possibly involved in the induction and the formation of the de novo developed vascular tissues occurring in poplar galls, a comparative transcript profiling of 21-day-old galls versus uninfected root of poplar was performed. Genes coding for transcription factors associated with procambium maintenance and vascular differentiation were shown to be differentially regulated, together with genes partaking in phytohormones biosynthesis and signalling. Specific signatures of transcripts associated to primary cell wall biosynthesis and remodelling, as well as secondary cell wall formation (cellulose, xylan and lignin) were revealed in the galls. Ultimately, we show that molecules derived from the monolignol and salicylic acid pathways and related to secondary cell wall deposition accumulate in mature galls.
A DNA Barcoding Survey of an Arctic Arthropod Community: Implications for Future Monitoring
Authors: Pentinsaari Mikko , Blagoev Gergin A. , Hogg Ian D. , Levesque-Beaudin Valerie , Perez Kate , Sobel Crystal N. , Vandenbrink Bryan , Borisenko Alex
Published: January 2020

Accurate and cost-effective methods for tracking changes in arthropod communities are needed to develop integrative environmental monitoring programs in the Arctic. To date, even baseline data on their species composition at established ecological monitoring sites are severely lacking. We present the results of a pilot assessment of non-marine arthropod diversity in a middle arctic tundra area near Ikaluktutiak (Cambridge Bay), Victoria Island, Nunavut, undertaken in 2018 using DNA barcodes. A total of 1264 Barcode Index Number (BIN) clusters, used as a proxy for species, were recorded. The efficacy of widely used sampling methods was assessed. Yellow pan traps captured 62% of the entire BIN diversity at the study sites. When complemented with soil and leaf litter sifting, the coverage rose up to 74.6%. Combining community-based data collection with high-throughput DNA barcoding has the potential to overcome many of the logistic, financial, and taxonomic obstacles for large-scale monitoring of the Arctic arthropod fauna.
Apolipoprotein B/Apolipoprotein A-I Ratio Is a Better Predictor of Cancer Mortality Compared with C-Reactive Protein: Results from Two Multi-Ethnic US Populations
Authors: Mazidi Mohsen , Katsiki Niki , Mikhailidis Dimitri P. , Radenkovic Dina , Pella Daniel , Banach Maciej
Published: January 2020

Background: There is a lack of evidence regarding the link between apolipoproteins and cancer mortality. By using two nationally representative samples of US adults, we prospectively evaluated the associations between apolipoprotein B (apoB) levels and apoB/apoA-I ratio with cancer mortality. We also examined the role of C-reactive protein (CRP) in these associations. Materials and Methods: Adults aged ≥20 years, enrolled in the 3rd National Health and Nutrition Examination Survey (NHANES III, 1988–1994) and continuous NHANES (2005–2010), and followed up to 31 December 2011, were included in the analysis. Multiple Cox regressions were applied to evaluate the associations between the variables of interest and cancer mortality. Results: Overall, 7695 participants were included (mean age: 49.2 years; 50.4% men, median follow-up: 19.1 years). In the fully adjusted model, participants in the highest quartile (Q4) of apoB/apoA-I had a significantly greater risk for cancer mortality (hazard ratio (HR): 1.40; 95% confidence interval (CI): 1.25–1.93) compared with those in the first quartile (Q1). In the same model, a positive and significant association between apoB levels and cancer mortality was observed for individuals in Q3 (HR: 1.12; 95% CI: 1.09–1.16) and Q4 (HR: 1.17; 95% CI: 1.09–1.25) compared with those in Q1. When CRP levels were added in the analysis, the apoB/apoA-I ratio, but not apoB levels, remained significantly related to cancer mortality (Q4 = HR: 1.17; 95% CI: 1.09–1.25). In contrast, CRP levels were not able to predict cancer death after correction for apoB/apoA-I ratio. Conclusions: In a large representative sample of the US adult population, the apoB/apoA-I ratio and apoB levels significantly predicted cancer mortality, independently of several cardiometabolic risk factors. The predictive value of apoB/apoA-I, but not apoB levels, remained significant after taking into account CRP, whereas CRP was not associated with cancer mortality after adjustment for apoB/apoA-I ratio. If further evidence supports our findings, apoA-I and apoB measurements could be considered in general healthcare policies.
Oncolytic Virotherapy with Myxoma Virus
Authors: Rahman Masmudur M. , McFadden Grant
Published: January 2020

Oncolytic viruses are one of the most promising novel therapeutics for malignant cancers. They selectively infect and kill cancer cells while sparing the normal counterparts, expose cancer- specific antigens and activate the host immune system against both viral and tumor determinants. Oncolytic viruses can be used as monotherapy or combined with existing cancer therapies to become more potent. Among the many types of oncolytic viruses that have been developed thus far, members of poxviruses are the most promising candidates against diverse cancer types. This review summarizes recent advances that are made with oncolytic myxoma virus (MYXV), a member of the Leporipoxvirus genus. Unlike other oncolytic viruses, MYXV infects only rabbits in nature and causes no harm to humans or any other non-leporid animals. However, MYXV can selectively infect and kill cancer cells originating from human, mouse and other host species. This selective cancer tropism and safety profile have led to the testing of MYXV in various types of preclinical cancer models. The next stage will be successful GMP manufacturing and clinical trials that will bring MYXV from bench to bedside for the treatment of currently intractable malignancies.
Characterization of Patients with Pulmonary Arterial Hypertension: Data from the Polish Registry of Pulmonary Hypertension (BNP-PL)
Authors: Kopeć Grzegorz , Kurzyna Marcin , Mroczek Ewa , Chrzanowski Łukasz , Mularek-Kubzdela Tatiana , Skoczylas Ilona , Kuśmierczyk Beata , Pruszczyk Piotr , Błaszczak Piotr , Lewicka Ewa , Karasek Danuta , Mizia-Stec Katarzyna , Tomaszewski Michał , Jacheć Wojciech , Ptaszyńska-Kopczyńska Katarzyna , Peregud-Pogorzelska Małgorzata , Doboszyńska Anna , Pawlak Agnieszka , Gąsior Zbigniew , Zabłocka Wiesława , Ryczek Robert , Widejko-Pietkiewicz Katarzyna , Waligóra Marcin , Darocha Szymon , Furdal Michał , Ciurzyński Michał , Kasprzak Jarosław D. , Grabka Marek , Kamiński Karol , Hoffman Piotr , Podolec Piotr , Torbicki Adam
Published: January 2020

Current knowledge of pulmonary arterial hypertension (PAH) epidemiology is based mainly on data from Western populations, and therefore we aimed to characterize a large group of Caucasian PAH adults of Central-Eastern European origin. We analyzed data of incident and prevalent PAH adults enrolled in a prospective national registry involving all Polish PAH centers. The estimated prevalence and annual incidence of PAH were 30.8/mln adults and 5.2/mln adults, respectively and they were the highest in females ≥65 years old. The most frequent type of PAH was idiopathic (n = 444; 46%) followed by PAH associated with congenital heart diseases (CHD-PAH, n = 356; 36.7%), and PAH associated with connective tissue disease (CTD-PAH, n = 132; 13.6%). At enrollment, most incident cases (71.9%) were at intermediate mortality risk and the prevalent cases had most of their risk factors in the intermediate or high risk range. The use of triple combination therapy was rare (4.7%). A high prevalence of PAH among older population confirms the changing demographics of PAH found in the Western countries. In contrast, we found: a female predominance across all age groups, a high proportion of patients with CHD-PAH as compared to patients with CTD-PAH and a low use of triple combination therapy.
The Effects of Hyperuricemia on the Prognosis of IgA Nephropathy are More Potent in Females
Authors: Oh Tae Ryom , Choi Hong Sang , Kim Chang Seong , Kang Kyung Pyo , Kwon Young Joo , Kim Sung Gyun , Ma Seong Kwon , Kim Soo Wan , Bae Eun Hui
Published: January 2020

Hyperuricemia is a potential risk factor for immunoglobulin A nephropathy (IgAN) progression but its sex-specific effects on IgAN progression remain unclear. This study aimed to determine the effect of serum uric acid on IgAN progression and whether its effect varied according to sex. A total of 4339 patients who diagnosed with IgAN by renal biopsy were retrospectively analyzed. We assessed the association of serum uric acid on IgAN progression using Kaplan–Meier survival analyses and Cox proportional hazards models. The study’s primary end point was IgAN progression that was defined as a 50% decline in the estimated glomerular filtration rate or the initiation of dialysis. On average, the serum uric acid levels were higher in the men than in the women. In the fully adjusted Cox proportional hazards model that considered all subjects, the risk of IgAN progression increased by about 25.6% for every 1 mg/dL increase in the baseline uric acid level. The serum uric acid level was an independent risk factor for IgAN progression in both sexes but its effect was more pronounced in the women (hazard ratio [HR], 1.383; confidence interval [CI],1.263 to 1.514; p < 0.001) than in the men (HR, 1.181; CI, 1.097 to 1.272; p < 0.001) (pinteraction < 0.001). A sensitivity analysis involving serum uric acid quartiles generated consistent and robust results. In conclusion, the serum uric acid level was an independent risk factor for IgAN progression and its effect was more pronounced among the women compared with that among the men.
The Effect of Mooring Line Parameters in Inducing Parametric Resonance on the Spar-Buoy Oscillating Water Column Wave Energy Converter
Authors: Giorgi Giuseppe , Gomes Rui P. F. , Bracco Giovanni , Mattiazzo Giuliana
Published: January 2020

Although it is widely accepted that accurate modeling of wave energy converters is essential for effective and reliable design, it is often challenging to define an accurate model which is also fast enough to investigate the design space or to perform extensive sensitivity analysis. In fact, the required accuracy is usually brought by the inclusion of nonlinearities, which are often time-consuming to compute. This paper provides a computationally efficient meshless nonlinear Froude–Krylov model, including nonlinear kinematics and an integral formulation of drag forces in six degrees of freedom, which computes almost in real-time. Moreover, a mooring system model with three lines is included, with each line comprising of an anchor, a jumper, and a clump weight. The mathematical model is used to investigate the highly-nonlinear phenomenon of parametric resonance, which has particularly detrimental effects on the energy conversion performance of the spar-buoy oscillating water column (OWC) device. Furthermore, the sensitivity on changes to jumper and clump-weight masses are discussed. It is found that mean drift and peak loads increase with decreasing line pre-tension, eventually leading to a reduction of the operational region. On the other hand, the line pre-tension does not affect power production efficiency, nor is it able to avoid or significantly limit the severity of parametric instability.
Elucidation of Conduction Mechanism in Graphene Nanoplatelets (GNPs)/Cement Composite Using Dielectric Spectroscopy
Authors: Goracci Guido , S. Dolado Jorge
Published: January 2020

Understanding the mechanisms that govern the conductive properties of multifunctional cement-materials is fundamental for the development of the new applications proposed to enhance the energy efficiency, safety and structural properties of smart buildings and infrastructures. Many fillers have been suggested to increase the electrical conduction in concretes; however, the processes involved are still not entirely known. In the present work, we investigated the effect of graphene nanoplatelets (1 wt% on the electrical properties of cement composites (OPC/GNPs). We found a decrease of the bulk resistivity in the composite associated to the enhancement of the charge transport properties in the sample. Moreover, the study of the dielectric properties suggests that the main contribution to conduction is given by water diffusion through the porous network resulting in ion conductivity. Finally, the results support that the increase of direct current in OPC/GNPs is due to pore refinement induced by graphene nanoplatelets.
Assessment of the Impact of the Addition of Nanoparticles on the Properties of Glass–Ionomer Cements
Authors: Gjorgievska Elizabeta , Nicholson John W. , Gabrić Dragana , Guclu Zeynep Asli , Miletić Ivana , Coleman Nichola J.
Published: January 2020

The aim of the study was to evaluate the effects of incorporation of Al2O3, ZrO2 and TiO2 nanoparticles into glass–ionomer cements (GICs). Two different GICs were used in the study. Four groups were prepared for each material: the control group (without nanoparticles) and three groups modified by the incorporation of nanoparticles at 2, 5 or 10 wt %, respectively. Cements were mixed and placed in moulds (4 mm × 6 mm); after setting, the samples were stored in saline (one day and one week). Compressive strengths were measured and the morphology of the fractured surfaces was analyzed by scanning electron microscopy. The elements released into the storage solutions were determined by Inductively coupled plasma-optical emission spectrometry (ICP-OES). Addition of nanoparticles was found to alter the appearance of cements as examined by scanning electron microscopy. Compressive strength increased with the addition of ZrO2 and especially TiO2 nanoparticles, whereas the addition of Al2O3 nanoparticles generally weakened the cements. The ion release profile of the modified cements was the same in all cases. The addition of Al2O3, ZrO2 and TiO2 nanoparticles into GICs is beneficial, since it leads to reduction of the microscopic voids in the set cement. Of these, the use of ZrO2 and TiO2 nanoparticles also led to increased compressive strength. Nanoparticles did not release detectable levels of ions (Al, Zr or Ti), which makes them suitable for clinical use.
Methods for Reducing the Toxicity of Metal and Metal Oxide NPs as Biomedicine
Authors: Długosz Olga , Szostak Krzysztof , Staroń Anita , Pulit-Prociak Jolanta , Banach Marcin
Published: January 2020

The rapid development of medicine has forced equally rapid progress in the field of pharmaceuticals. In connection with the expensive and time-consuming process of finding new drugs, great emphasis is put on the design and use of metal and metal oxides nanoparticles in nanomedicine. The main focus is on comprehensive presentation of both physicochemical properties and the possibilities of using, in particular, silver (Ag) and gold (Au) nanoparticles, as well as zinc oxide (ZnO) and titanium oxide (TiO2) nanoparticles as drug carriers and in the treatment of cancer. An important element of this subject is the possibility of occurrence of toxic effects of these nanoparticles. For this reason, possible mechanisms of toxic actions are presented, as well as methods used to reduce their toxicity to ensure the safety of drug carriers based on these nanostructures.
Multi-Terminal Transistor-Like Devices Based on Strongly Correlated Metallic Oxides for Neuromorphic Applications
Authors: Fernández-Rodríguez Alejandro , Alcalà Jordi , Suñe Jordi , Mestres Narcis , Palau Anna
Published: January 2020

Memristive devices are attracting a great attention for memory, logic, neural networks, and sensing applications due to their simple structure, high density integration, low-power consumption, and fast operation. In particular, multi-terminal structures controlled by active gates, able to process and manipulate information in parallel, would certainly provide novel concepts for neuromorphic systems. In this way, transistor-based synaptic devices may be designed, where the synaptic weight in the postsynaptic membrane is encoded in a source-drain channel and modified by presynaptic terminals (gates). In this work, we show the potential of reversible field-induced metal-insulator transition (MIT) in strongly correlated metallic oxides for the design of robust and flexible multi-terminal memristive transistor-like devices. We have studied different structures patterned on YBa2Cu3O7−δ films, which are able to display gate modulable non-volatile volume MIT, driven by field-induced oxygen diffusion within the system. The key advantage of these materials is the possibility to homogeneously tune the oxygen diffusion not only in a confined filament or interface, as observed in widely explored binary and complex oxides, but also in the whole material volume. Another important advantage of correlated oxides with respect to devices based on conducting filaments is the significant reduction of cycle-to-cycle and device-to-device variations. In this work, we show several device configurations in which the lateral conduction between a drain-source channel (synaptic weight) is effectively controlled by active gate-tunable volume resistance changes, thus providing the basis for the design of robust and flexible transistor-based artificial synapses.
Non-Destructive Evaluation of Rock Bolt Grouting Quality by Analysis of Its Natural Frequencies
Authors: Bačić Mario , Kovačević Meho Saša , Jurić Kaćunić Danijela
Published: January 2020

Grouted rock bolts represent one of the most used elements for rock mass stabilization and reinforcement and the grouting quality has a crucial role in the load transfer mechanism. At the same time, the grouting quality as well as the grouting procedures are the least controlled in practice. This paper deals with the non-destructive investigation of grouting percentage through an analysis of the rock bolt’s natural frequencies after applying an artificial longitudinal impulse to its head by using a soft-steel hammer as a generator. A series of laboratory models, with different positions and percentages of the grouted section, simulating grouting defects, were tested. A comprehensive statistical analysis was conducted and a high correlation between the grouting percentage and the first three natural frequencies of rock bolt models has been established. After validation of FEM numerical models based on experimentally obtained values, a further analysis includes consideration of grout stiffness variation and its impact on natural frequencies of rock bolt.
Combined Fuzzy and Genetic Algorithm for the Optimisation of Hybrid Composite-Polymer Joints Obtained by Two-Step Laser Joining Process
Authors: Ponticelli Gennaro Salvatore , Lambiase Francesco , Leone Claudio , Genna Silvio
Published: January 2020

In the present work, genetic algorithms and fuzzy logic were combined to model and optimise the shear strength of hybrid composite-polymer joints obtained by two step laser joining process. The first step of the process consists of a surface treatment (cleaning) of the carbon fibre-reinforced polymer (CFRP) laminate, by way of a 30 W nanosecond laser. This phase allows removing the first matrix layer from the CFRP and was performed under fixed process parameters condition. In the second step, a diode laser was adopted to join the CFRP to polycarbonate (PC) sheet by laser-assisted direct joining (LADJ). The experimentation was performed adopting an experimental plan developed according to the design of experiment (DOE) methodology, changing the laser power and the laser energy. In order to verify the cleaning effect, untreated laminated were also joined and tested adopting the same process conditions. Analysis of variance (ANOVA) was adopted to detect the statistical influence of the process parameters. Results showed that both the laser treatment and the process parameters strongly influence the joint performances. Then, an uncertain model based on the combination of fuzzy logic and genetic algorithms was developed and adopted to find the best process parameters’ set able to give the maximum joint strength against the lowest uncertainty level. This type of approach is especially useful to provide information about how much the precision of the model and the process varies by changing the process parameters.
Porous Poly(Hexamethylene Biguanide) Hydrochloride Loaded Silk Fibroin Sponges with Antibacterial Function
Authors: Liang Ahui , Zhang Min , Luo Hong , Niu Longxing , Feng Yanfei , Li Mingzhong
Published: January 2020

In order to endue silk fibroin (SF) sponges with antibacterial function, positively charged poly(hexamethylene biguanide) hydrochloride (PHMB) was incorporated in SF through electrostatic interaction and by freeze-drying technique. The influence of PHMB on the structure and antibacterial activities of SF sponges was investigated. The zeta potential of SF was increased significantly when PHMB was incorporated in SF. The pores with size from 80 to 300 µm and the microscale holes in the pore walls within PHMB-loaded SF sponges provided the channels of PHMB release. The PHMB loaded in the porous sponges showed continuous and slow release for up to 20 days. Effective growth inhibition of both Escherichia coli and Staphylococcus aureus was achieved when the mass ratio of PHMB/SF was higher than 2/100. These results suggest that the porous PHMB/SF sponges have the potential to be used as a novel wound dressing for open skin wounds.
Assessment of Alkali–Silica Reactivity of Aggregates by Concrete Expansion Tests in Alkaline Solutions at 38 °C
Authors: Bavasso Irene , Costa Umberto , Mangialardi Teresa , Paolini Antonio Evangelista
Published: January 2020

A new accelerated concrete prism expansion test at 38 °C (accelerated CPT) is proposed for assessing the alkali-reactivity of concrete aggregates. In this test, concrete prisms with a standardized mix composition and different alkali contents are immersed in alkaline solutions with compositions simulating the pore liquid of hardened concretes. The concrete prism expansion test at 38 °C and RH > 95% (traditional CPT) was taken as a reference test, in order to define the appropriate expansion limit criterion for the proposed accelerated CPT. Three natural aggregates of known field performance and different alkali–silica reactivity were tested. The compositions of alkaline solutions were designed by assuming total dissolution of cement alkalis and taking a ratio between the mass fractions of effective water consumed by cement hydration and of alkalis uptaken by cement hydrates equal to unity. This simplified approach was found in an acceptable agreement with literature empirical equations correlating pore solution alkalinity of hardened Portland cement mixes with total alkali content of cement. Elaboration of expansion data through both pass-fail and threshold alkali level (TAL)-evaluation approaches indicated that, for the accelerated CPT, an expansion limit criterion of 0.04% after 120 days of testing in alkaline solutions is appropriate to evaluate the aggregate alkali reactivity congruently with the traditional CPT. Use of the proposed test method in place of the traditional CPT would reduce the test duration from 365 to 120 days.
Estimation of an Upper Bound to the Value of the Step Potentials in Two-Layered Soils from Grounding Resistance Measurements
Authors: Moreno Jorge , Simon Pascual , Faleiro Eduardo , Asensio Gabriel , Fernandez Jose Antonio
Published: January 2020

Due to the constant updating of regulatory standards on safety issues in electrical installations, limits are established for the maximum step potential that an installation can hold in a ground fault situation. In this paper, an upper bound to the maximum value of the step potentials arising in the soil surface when a fault takes place in a grounded electrical installation is estimated by means of a simple procedure. The direct measurement of the grounding electrode resistance together with some information about the soil resistivity and the knowledge of characteristic parameters of the electrode are used for the calculation of that upper bound. The procedure is tested at numerical simulation level by using different electrodes in several different scenarios corresponding to two-layered soils with different resistivity ratios. The dependency of the calculated upper bound with the electrode burial depth is also studied. Finally, a real case study is presented, and the results of the field measurements are shown as an example of the validity of the procedure.
The Influence of Modification with Natural Fillers on the Mechanical Properties of Epoxy Adhesive Compositions after Storage Time
Authors: Miturska Izabela , Rudawska Anna , Müller Miroslav , Valášek Petr
Published: January 2020

This article presents the initial test results examining basic technological factors, such as type of modifying agent and seasoning time, which influence properties of adhesive epoxide compositions. The aim of the study was to prepare adhesive compositions with 2% content of the selected natural fillers (montmorillonite NanoBent ZR-2, ground chalk (powder)—CaCO3, and activated carbon powder C) and to examine their strength properties. A polymeric matrix used to prepare an adhesive composition consisted of the epoxide resins used in industry: Epidian 5 and Epidian 53 cured by addition of an aminomethyl group, where curing occurred through the Mannich reaction. A composition of epoxide resins with a curing agent and without any modifying agents was used as reference. The examinations described in the present article aimed to show the significance of the impact of the fillers used on the strength properties of the examined compositions. A fracture surface of epoxide adhesive compositions modified with the selected fillers was tested by means of a scanning electron microscope.
Dentin Bonding of TheraCal LC Calcium Silicate Containing an Acidic Monomer: An In Vitro Study
Authors: Kim Young Kyung , Hong Mi-Hee , Kwon Tae-Yub
Published: January 2020

The purpose of this study was to evaluate whether the incorporation of an acidic monomer into the pulp-capping material TheraCal LC, which has a weak dentin bonding, increases the shear bond strength (SBS) to dentin. Di-2-hydroxyethyl methacryl hydrogenphosphate was incorporated into the material at 0.0 (TL0, control), 5.0 (TL5), and 10.0 (TL10) wt%. The water contact angle (CA) and mechanical properties for each material were also studied (n = 6). Debonding was performed at two different times (immediate and after 24 h) (n = 12). Hydroxyl and calcium ion releases in water at 37 °C were monitored up to 28 days (n = 6). The addition of the acidic monomer decreased the CAs (p < 0.001) and increased the flexural moduli (p < 0.001). The debonding time did not significantly affect the SBS values (p = 0.600). The TL10 group exhibited the highest SBS values, followed by the TL5 group. The TL10 group released significantly more calcium ions than the other two groups from 3 days (p < 0.05). The incorporation of the acidic monomer at 10.0 wt% into TheraCal LC enhanced bonding to dentin, while not negatively affecting the mechanical properties and ion-leaching capacity of the material.
Study on Short Fatigue Crack Behaviour of LZ50 Steel Under Non-Proportional Loading
Authors: Yang Bing , Liao Zhen , Xiao Shoune , Yang Guangwu , Zhu Tao , Zhang Xiangning
Published: January 2020

The low cycle fatigue tests using the replica technique for LZ50 steel under non-proportional cyclic loading were carried out, and eight groups of effective test data were obtained. The evolution behaviour of short cracks was studied based on the effective short cracks criterion. The results show that short cracks generally originate in the grain or along the grain boundary. At the microstructural short crack stage, the crack propagation is influenced strongly by the microstructure of the material, and the growth rate of the short crack slows down several times according to the number of obstacles encountered. At the physical short crack stage, the crack propagation breaks through the banded structure of pearlite. Thus, the dominant effective short fatigue crack is formed, and the crack growth rate increases rapidly. Based on the modified parameters of the uniaxial short crack model, an approach is presented to calculate the growth rate of short cracks under multi-axial non-proportional loadings, and the new model can consider the non-proportional factor F. The fitting results of the multi-axial microstructural obstacles model are compared with test data. The comparison results show that this model can reflect the trend of short fatigue crack propagation rate under non-proportional loadings.
Influence of Single- and Multi-Wall Carbon Nanotubes on Magnetohydrodynamic Stagnation Point Nanofluid Flow over Variable Thicker Surface with Concave and Convex Effects
Authors: Shafiq Anum , Khan Ilyas , Rasool Ghulam , Sherif El-Sayed M. , Sheikh Asiful H.
Published: January 2020

This paper reports a theoretical study on the magnetohydrodynamic flow and heat exchange of carbon nanotubes (CNTs)-based nanoliquid over a variable thicker surface. Two types of carbon nanotubes (CNTs) are accounted for saturation in base fluid. Particularly, the single-walled and multi-walled carbon nanotubes, best known as SWCNTs and MWCNTs, are used. Kerosene oil is taken as the base fluid for the suspension of nanoparticles. The model involves the impact of the thermal radiation and induced magnetic field. However, a tiny Reynolds number is assumed to ignore the magnetic induction. The system of nonlinear equations is obtained by reasonably adjusted transformations. The analytic solution is obtained by utilizing a notable procedure called optimal homotopy analysis technique (O-HAM). The impact of prominent parameters, such as the magnetic field parameter, Brownian diffusion, Thermophoresis, and others, on the dimensionless velocity field and thermal distribution is reported graphically. A comprehensive discussion is given after each graph that summarizes the influence of the respective parameters on the flow profiles. The behavior of the friction coefficient and the rate of heat transfer (Nusselt number) at the surface (y = 0) are given at the end of the text in tabular form. Some existing solutions of the specific cases have been checked as the special case of the solution acquired here. The results indicate that MWCNTs cause enhancement in the velocity field compared with SWCNTs when there is an increment in nanoparticle volume fraction. Furthermore, the temperature profile rises with an increment in radiation estimator for both SWCNT and MWCNT and, finally, the heat transfer rate lessens for increments in the magnetic parameter for both types of nanotubes.
Comparison of the Analgesic Effect of Ropivacaine with Fentanyl and Ropivacaine Alone in Continuous Epidural Infusion for Acute Herpes Zoster Management: A Retrospective Study
Authors: Kang Hee Yong , Lee Chung Hun , Choi Sang Sik , Lee Mi Kyoung , Lee Yeon Joo , Park Jong Sun
Published: January 2020

Background and Objectives: Currently, few studies have reported the effects of opioids during continuous epidural infusion (CEI) to control pain owing to herpes zoster (HZ). This study aimed to retrospectively compare the effectiveness of epidural opioids in the treatment of acute HZ pain. Materials and Methods: We reviewed medical records of 105 patients who were divided into two groups: R group (CEI with ropivacaine) and RF group (CEI with ropivacaine and fentanyl). Clinical efficacy was evaluated using the numeric rating scale (NRS) score for 6 months after the procedures. We compared the percentage of patients with complete remission in each group. We investigated the complication rates during CEI. Results: No significant differences in the NRS scores were observed between the two groups in the 6-month period. The adjusted odds ratio (OR) for patients included in the complete remission was 0.6 times lower in the RF group than in the R group (95% confidence interval: 0.22–1.71, p = 0.35). The OR for complications during CEI was higher in the RF group than in the R group. However, the difference was not statistically significant. Conclusions: No difference was observed in the management of HZ pain and the prevention of postherpetic neuralgia between the two groups. The incidence of complications tended to be higher in the RF group than in the R group.
When Combined with Colistin, an Otherwise Ineffective Rifampicin–Linezolid Combination Becomes Active in Escherichia coli, Pseudomonas aeruginosa, and Acinetobacter baumannii
Authors: Armengol Eva , Asunción Teresa , Viñas Miguel , Sierra Josep Maria
Published: January 2020

The synergistic action of colistin, with two antibiotics active in Gram-positive bacteria but unable to kill gram negatives (linezolid and rifampicin), was investigated, since triple combinations are emerging as a tool to overtake multidrug resistance. Checkerboard determinations demonstrated that, when combined with colistin, the combination of linezolid and rifampicin turns active in multidrug-resistant Escherichia coli, Pseudomonas aeruginosa, and Acinetobacter baumannii. Thus, the presence of sublethal concentrations of colistin resulted in a strongly synergistic interaction between these two drugs. Moreover, the minimum inhibitory concentrations of linezolid–rifampicin combinations in the presence of colistin were lower than the maximal concentrations of these antimicrobials ain blood. These findings suggest the use of this triple combination as an effective treatment of multidrug-resistant (MDR) bacterial infections.
Zn Complex of Diaminedithiol Tetradentate Ligand as a Stable Precursor for 99mTc-Labeled Compounds
Authors: Oshikiri Shinobu , Uehara Tomoya , Suzuki Hiroyuki , Koike-Satake Miho , Hino Akihiro , Arano Yasushi
Published: January 2020

The diaminedithiol (N2S2) tetradentate ligand constitutes a useful chelating molecule for preparing 99mTc-labeled compounds of high in vivo stability in high radiochemical yields. However, since the thiol groups in the N2S2 ligand are easy to be oxidized to disulfide bonds, they need to be protected with an appropriate protecting group, which hinders the broad applications of the N2S2 ligand for radiopharmaceuticals. In this study, a Zn chelate of N2S2 was evaluated as a precursor for purification-free 99mTc-labeled N2S2 under the mild and simple procedure. Zn-N2S2 was prepared by reacting Zn acetate with N2S2, and the Zn-N2S2 remained stable under aerobic conditions at room temperature. 99mTc-N2S2 was obtained over 90% radiochemical yields at room temperature by a one-pot reaction, consisting of Zn-N2S2 (10−5 M), 99mTcO4−, ethylenediaminetetraacetic acid (EDTA), and a reducing agent (Sn2+) at pH = 5.5 to 7.5. 99mTc-N2S2 was also obtained over 90% radiochemical yields when the reaction was conducted in the presence of an equimolar amount of IgG antibody. These findings indicate the Zn complex of N2S2 ligand constitutes a stable and useful precursor to prepare 99mTc-labeled N2S2 compounds in high yields under the mild and simple procedure.